{"id": "PMID:459569", "title": "Stochastic studies of aging and mortality in multicellular organisms. II. The finite theory.", "content": "We extend the development of a quantitative phenomenological theory of aging rooted in order theory. An organism is represented abstractly by a chain with a finite number of links. Each link corresponds to a possible rate-limiting event or process in senescence. A link is said to break when the corresponding event occurs or the corresponding process goes to completion. The chain is said to break, and the organism subsequently to perish, when the first link breaks, whichever link that might be. Two models are introduced to describe the failure of an arbitrary link. The first requires that a link break only after sustaining a fixed amount of deterioration; the second associates a non-zero probability of failure with each level of wear. The net deterioration of an intact chain is taken, crudely, to correspond to the decline in physiological vitality sustained by an organism during senescence. Failure of an arbitrary link is described in both models by a Markov process. The corresponding mortality rate derived in each instance describes aspects of available empirical data which cannot be accounted for by either the Gompertz or power-law relations. The decline in vitality is shown in both cases to be linear over time intervals of practical interest. The influence of temperature on the senescence of poikilotherms is briefly examined. We describe the effect of temperature both on longevity and the decline in vitality; indicate how substantial discrepancies can arise in the calculation of a macroscopic, effective, activation enthalpy; and lend theoretical support to the existence of temperature--memory effects in senescence.", "contents": "Stochastic studies of aging and mortality in multicellular organisms. II. The finite theory. We extend the development of a quantitative phenomenological theory of aging rooted in order theory. An organism is represented abstractly by a chain with a finite number of links. Each link corresponds to a possible rate-limiting event or process in senescence. A link is said to break when the corresponding event occurs or the corresponding process goes to completion. The chain is said to break, and the organism subsequently to perish, when the first link breaks, whichever link that might be. Two models are introduced to describe the failure of an arbitrary link. The first requires that a link break only after sustaining a fixed amount of deterioration; the second associates a non-zero probability of failure with each level of wear. The net deterioration of an intact chain is taken, crudely, to correspond to the decline in physiological vitality sustained by an organism during senescence. Failure of an arbitrary link is described in both models by a Markov process. The corresponding mortality rate derived in each instance describes aspects of available empirical data which cannot be accounted for by either the Gompertz or power-law relations. The decline in vitality is shown in both cases to be linear over time intervals of practical interest. The influence of temperature on the senescence of poikilotherms is briefly examined. We describe the effect of temperature both on longevity and the decline in vitality; indicate how substantial discrepancies can arise in the calculation of a macroscopic, effective, activation enthalpy; and lend theoretical support to the existence of temperature--memory effects in senescence."}
{"id": "PMID:459571", "title": "Hydrocortisone: a specific modulator of in vitro cell proliferation and aging.", "content": "The effects of hydrocortisone on the proliferative activity of various vertebrate fibroblast-like cell lines have been measured. The proliferative activity of human fetal lung-derived cell lines, such as WI-38, and those of human fetal foreskin origin were always enhanced by the hormone. The addition of hydrocortisone to a number of other vertebrate cell lines grown under identical conditions showed growth inhibition. We also determined that the stimulation of WI-38 cell growth by hydrocortisone was a specific steroid-cell interaction by testing the growth promoting activity of several steroids with molecular structures similar to that of hydrocortisone. This observation was further supported by the presence of high effinity glucocorticoid binding sites in WI-38 cells whose concentration per cell was found to decrease with increased in vitro age. The results suggest that the proliferative response to hydrocortisone shows a cell-type specificity as well as a steroid-molecular structure specificity. The response appears to be mediated by high affinity glucocorticoid binding sites.", "contents": "Hydrocortisone: a specific modulator of in vitro cell proliferation and aging. The effects of hydrocortisone on the proliferative activity of various vertebrate fibroblast-like cell lines have been measured. The proliferative activity of human fetal lung-derived cell lines, such as WI-38, and those of human fetal foreskin origin were always enhanced by the hormone. The addition of hydrocortisone to a number of other vertebrate cell lines grown under identical conditions showed growth inhibition. We also determined that the stimulation of WI-38 cell growth by hydrocortisone was a specific steroid-cell interaction by testing the growth promoting activity of several steroids with molecular structures similar to that of hydrocortisone. This observation was further supported by the presence of high effinity glucocorticoid binding sites in WI-38 cells whose concentration per cell was found to decrease with increased in vitro age. The results suggest that the proliferative response to hydrocortisone shows a cell-type specificity as well as a steroid-molecular structure specificity. The response appears to be mediated by high affinity glucocorticoid binding sites."}
{"id": "PMID:459572", "title": "Age-dependent gene induction in quail oviduct. VII. Alteration of DNA polymerase activities in response to progesterone treatment.", "content": "The changes of DNA synthesis as well as the alterations of DNA polymerase alpha and beta have been determined in oviducts from immature (35 days old), mature (about 300 days old) and senescent (about 3 years old) quails in response to diethylstilbestrol (DES). The DNA synthesis in oviducts from immature quails is strongly stimulated by DES; after 12 days, values equivalent to those determined in mature and senescent animals are reached. The DNA synthesis in immature oviducts can be reversibly blocked by progesterone administration; no influence is observed in the case of DNA synthesis in mature as well as senescent animals. During DNA synthesis in immature oviducts, stimulated with DES, DNA polymerase alpha is strongly induced. Co-administration of progesterone blocks this enzyme induction in a reversible and strong way. The activity of DNA polymerase alpha is identical in mature and in immature animals (after DES treatment for 15 days); the activity of the same enzyme in senescent animals is about 40% lower than the values found in the younger quails. The activity of DNA polymerase beta is not altered if the animals are treated with DES or with DES and progersterone; however, the basic level of the enzyme in senescent animals in 50% lower than in immature or in mature animals.", "contents": "Age-dependent gene induction in quail oviduct. VII. Alteration of DNA polymerase activities in response to progesterone treatment. The changes of DNA synthesis as well as the alterations of DNA polymerase alpha and beta have been determined in oviducts from immature (35 days old), mature (about 300 days old) and senescent (about 3 years old) quails in response to diethylstilbestrol (DES). The DNA synthesis in oviducts from immature quails is strongly stimulated by DES; after 12 days, values equivalent to those determined in mature and senescent animals are reached. The DNA synthesis in immature oviducts can be reversibly blocked by progesterone administration; no influence is observed in the case of DNA synthesis in mature as well as senescent animals. During DNA synthesis in immature oviducts, stimulated with DES, DNA polymerase alpha is strongly induced. Co-administration of progesterone blocks this enzyme induction in a reversible and strong way. The activity of DNA polymerase alpha is identical in mature and in immature animals (after DES treatment for 15 days); the activity of the same enzyme in senescent animals is about 40% lower than the values found in the younger quails. The activity of DNA polymerase beta is not altered if the animals are treated with DES or with DES and progersterone; however, the basic level of the enzyme in senescent animals in 50% lower than in immature or in mature animals."}
{"id": "PMID:459573", "title": "Developmental restrictions on transcription: determinants of the developmental program and their role in aging.", "content": "A developing plant system, the soybean hypocotyl has been used to investigate early transcription events which restrict auxin induced cellular proliferation to the appropriate developmental stage. Auxin treatment of 4-day old seedlings resulted in an early (6 hour) activation of chromatin-bound RNA polymerase activity wihich approached 200% of control values by 18 hours. This occurred without a detectable alteration in chromatin template capacity (assayed with exogenous RNA polymerase) and resulted in the synthesis of \"induced RNA transcripts\" as determined in vitro by nearest neighbor analysis. In contrast, auxin treatment of unresponsive 8-day old seedlings did not alter the chromatin-bound RNA polymerase activity. Hormonal activation did, however, result in the exposure of \"induced template\" regions in chromatin which could only be transcribed in vitro if exogenous RNA polymerase was included in the transcription reaction. Isoelectric focusing of the endogenous chromatin-bound and soluble RNA polymerase enzymes from successive developmental stages revealed that the chromatin-bound enzymes at the 2-day stage were first released from the chromatin complex and could be recovered in the soluble pool (4-day stage). This was followed by a gradual disappearance of these subspecies (6-day stage) until only a limited ensemble of RNA polymerase subspecies remained bound to chromatin and free in the soluble pool (8-day stage). Similar analyses of both the bound and free enzymes at the 4 and 8-day stages following auxin treatment revealed that the 4-day soluble enzymes could be induced to rebind to the chromatin complex in a defined sequence after hormone treatment while those of the 8-day hypocotyl were unable to do so. These developmental events indicate that the select loss of certain RNA polymerase subspecies serves to restrict the hormone responsivness of this tissue to the early developmental stages. Such restrictions could thus commit the constituent hypocotyl cells to their terminal post-mitotic phase of development.", "contents": "Developmental restrictions on transcription: determinants of the developmental program and their role in aging. A developing plant system, the soybean hypocotyl has been used to investigate early transcription events which restrict auxin induced cellular proliferation to the appropriate developmental stage. Auxin treatment of 4-day old seedlings resulted in an early (6 hour) activation of chromatin-bound RNA polymerase activity wihich approached 200% of control values by 18 hours. This occurred without a detectable alteration in chromatin template capacity (assayed with exogenous RNA polymerase) and resulted in the synthesis of \"induced RNA transcripts\" as determined in vitro by nearest neighbor analysis. In contrast, auxin treatment of unresponsive 8-day old seedlings did not alter the chromatin-bound RNA polymerase activity. Hormonal activation did, however, result in the exposure of \"induced template\" regions in chromatin which could only be transcribed in vitro if exogenous RNA polymerase was included in the transcription reaction. Isoelectric focusing of the endogenous chromatin-bound and soluble RNA polymerase enzymes from successive developmental stages revealed that the chromatin-bound enzymes at the 2-day stage were first released from the chromatin complex and could be recovered in the soluble pool (4-day stage). This was followed by a gradual disappearance of these subspecies (6-day stage) until only a limited ensemble of RNA polymerase subspecies remained bound to chromatin and free in the soluble pool (8-day stage). Similar analyses of both the bound and free enzymes at the 4 and 8-day stages following auxin treatment revealed that the 4-day soluble enzymes could be induced to rebind to the chromatin complex in a defined sequence after hormone treatment while those of the 8-day hypocotyl were unable to do so. These developmental events indicate that the select loss of certain RNA polymerase subspecies serves to restrict the hormone responsivness of this tissue to the early developmental stages. Such restrictions could thus commit the constituent hypocotyl cells to their terminal post-mitotic phase of development."}
{"id": "PMID:459574", "title": "Factors influencing styles of medical practice: the use of psychiatric referrals by non-psychiatric physicians.", "content": "There is substantial variation between physicians in the extent to which they employ psychiatric referral as a mechanism for dealing with their patients who have psychiatric problems. A variety of factors, including personal characteristics, professional attitudes, training and practice characteristics, were hypothesized to influence styles of practice in this area of medicine. A regression equation with a multiple R of .71 indicated that personal characteristics and attitudes were particularly strongly related, but that practice and patient characteristics as well as training also play some role in influencing a physician's behavior.", "contents": "Factors influencing styles of medical practice: the use of psychiatric referrals by non-psychiatric physicians. There is substantial variation between physicians in the extent to which they employ psychiatric referral as a mechanism for dealing with their patients who have psychiatric problems. A variety of factors, including personal characteristics, professional attitudes, training and practice characteristics, were hypothesized to influence styles of practice in this area of medicine. A regression equation with a multiple R of .71 indicated that personal characteristics and attitudes were particularly strongly related, but that practice and patient characteristics as well as training also play some role in influencing a physician's behavior."}
{"id": "PMID:459575", "title": "Physician awareness of economic factors in clinical decision-making.", "content": "Physicians' awareness of economic factors in clinical decision-making at one large urban university medical center was studied by a 50-item questionnaire. Their dollar estimates were considered correct if within +/- 20 per cent of the true October 1976 figure. Eighty-one per cent of the house staff and all of the attendings correctly estimated the daily semi-private room rate, but only 15 per cent of each group correctly estimated the charge for a serum potassium. Roughly half of the questions concerning various third-party benefit plans were answered correctly. These results are consistent with those of the few previous studies. If the findings are generally applicable, they may suggest that a directed teaching program in simple economic facts and principles may be useful at all levels of physician training.", "contents": "Physician awareness of economic factors in clinical decision-making. Physicians' awareness of economic factors in clinical decision-making at one large urban university medical center was studied by a 50-item questionnaire. Their dollar estimates were considered correct if within +/- 20 per cent of the true October 1976 figure. Eighty-one per cent of the house staff and all of the attendings correctly estimated the daily semi-private room rate, but only 15 per cent of each group correctly estimated the charge for a serum potassium. Roughly half of the questions concerning various third-party benefit plans were answered correctly. These results are consistent with those of the few previous studies. If the findings are generally applicable, they may suggest that a directed teaching program in simple economic facts and principles may be useful at all levels of physician training."}
{"id": "PMID:459576", "title": "Patient race and physician performances: quality of medical care, hospital admissions and hospital stays.", "content": "The study has attempted to determine the extent of the relationship between patient race and physicians' performances in patient care. The sample of the study consisted of 3175 hospital episode of patients discharged from 22 short-term general hospitals in the state of Hawaii. The episodes were derived from 15 major diagnostic categories. Physicians performances were measured on the basis of the quality of medical care provided, the appropriateness of hospital admissions, and the appropriateness of hospital stays, including understays and overstays. The study has found: 1) that patient race had very limited influence on physicians' performances: the quality of medical care, the appropriateness of hospital admissions, and the appropriateness of hospital stays; 2) that Asian-Americans receive medical care equal to that of the white Americans (once they had access to the health care systems), at least in the state of Hawaii; 3) that among Asian-Americans, there was no distinct difference in medical care received by Japanese, Chinese, and Filipino; 4) that there was clear evidence of racial mutual selection between patients and physicians; and 5) that patients treated by the physicians with the same racial/ethnic backgrounds received care neither superior nor inferior to the care received by patients from the physicians with different backgrounds.", "contents": "Patient race and physician performances: quality of medical care, hospital admissions and hospital stays. The study has attempted to determine the extent of the relationship between patient race and physicians' performances in patient care. The sample of the study consisted of 3175 hospital episode of patients discharged from 22 short-term general hospitals in the state of Hawaii. The episodes were derived from 15 major diagnostic categories. Physicians performances were measured on the basis of the quality of medical care provided, the appropriateness of hospital admissions, and the appropriateness of hospital stays, including understays and overstays. The study has found: 1) that patient race had very limited influence on physicians' performances: the quality of medical care, the appropriateness of hospital admissions, and the appropriateness of hospital stays; 2) that Asian-Americans receive medical care equal to that of the white Americans (once they had access to the health care systems), at least in the state of Hawaii; 3) that among Asian-Americans, there was no distinct difference in medical care received by Japanese, Chinese, and Filipino; 4) that there was clear evidence of racial mutual selection between patients and physicians; and 5) that patients treated by the physicians with the same racial/ethnic backgrounds received care neither superior nor inferior to the care received by patients from the physicians with different backgrounds."}
{"id": "PMID:459577", "title": "Care process and patient outcome in diabetes mellitus.", "content": "The relationship between the process of medical care and patient outcome is a central issue in health services research. We examined this relationship in 244 patients with adult-onset diabetes mellitus, who were under the care of private internists and family physicians. Process measures included physician awareness of patients' concerns, communication of information from physician to patient, medication-taking behavior, physician adherence to minimum care criteria, and extent of patient utilization of services. Outcomes measured during and after a 6-month follow-up period, included diabetic control status and patient satisfaction with medical care. Potentially confounding variables included practice and physician characteristics, patient demographic characteristics, and measures of disease severity. There was a small statistically significant correlation between physician awareness and control status, but the association was not maintained when controlling for other variables. Communication of information from physician to patient was significantly (p less than .005) associated with satisfaction in the multiple regression analysis but explained only 4 per cent of the variance in patient satisfaction. Thus, in patients under treatment for diabetes, there was little association between certain measures of care process and patient outcome. We suggest that process and outcome assessments are distinct but complementary aspects of quality of care.", "contents": "Care process and patient outcome in diabetes mellitus. The relationship between the process of medical care and patient outcome is a central issue in health services research. We examined this relationship in 244 patients with adult-onset diabetes mellitus, who were under the care of private internists and family physicians. Process measures included physician awareness of patients' concerns, communication of information from physician to patient, medication-taking behavior, physician adherence to minimum care criteria, and extent of patient utilization of services. Outcomes measured during and after a 6-month follow-up period, included diabetic control status and patient satisfaction with medical care. Potentially confounding variables included practice and physician characteristics, patient demographic characteristics, and measures of disease severity. There was a small statistically significant correlation between physician awareness and control status, but the association was not maintained when controlling for other variables. Communication of information from physician to patient was significantly (p less than .005) associated with satisfaction in the multiple regression analysis but explained only 4 per cent of the variance in patient satisfaction. Thus, in patients under treatment for diabetes, there was little association between certain measures of care process and patient outcome. We suggest that process and outcome assessments are distinct but complementary aspects of quality of care."}
{"id": "PMID:459578", "title": "A new intermediate dental outcome measure: amalgam replacement rate.", "content": "The length of time amalgam restorations last before replacement was investigated for use as an intermediate outcome measure in utilization review and quality assurance studies. Based on record data from 37 general dental practices, a determination was made of the average percentage of two- or three-surface amalgams receiving another service at 6, 12, and 24 months from the date of insertion. After 2 years, approximately 13 per cent of the amalgams were replaced, and the estimated median life time for amalgams was between 10 and 14 years. The variation in replacement rates among practices was substantial but was not explained by the technical quality of restorations or several practice characteristics.", "contents": "A new intermediate dental outcome measure: amalgam replacement rate. The length of time amalgam restorations last before replacement was investigated for use as an intermediate outcome measure in utilization review and quality assurance studies. Based on record data from 37 general dental practices, a determination was made of the average percentage of two- or three-surface amalgams receiving another service at 6, 12, and 24 months from the date of insertion. After 2 years, approximately 13 per cent of the amalgams were replaced, and the estimated median life time for amalgams was between 10 and 14 years. The variation in replacement rates among practices was substantial but was not explained by the technical quality of restorations or several practice characteristics."}
{"id": "PMID:459589", "title": "[Giant cells arteritis. A disease previously reported as various syndromes (author's transl)].", "content": "Thirteen cases of giant cell arteritis admitted to a Department of Internal Medicine between 1962 and 1977 are reviewed. Giant cell arteritis exhibited different clinical forms, including: temporal arteritis, rheumatic polymyalgia, syndrome of the supra-aortic trunks, cranial arteritis in its ophthalmic and neurologic forms, and generalized arteritis. On the basis of the clinical data, overall examinations, laboratory tests, X-rays, angiograms and histopathologic findings the authors conclude that a single disease is involved. The fact that various of the symptoms occur simultaneously in different patients and that all of the clinical forms have a common pathologic basis points toward giant cell arteritis with different clinical manifestations.", "contents": "[Giant cells arteritis. A disease previously reported as various syndromes (author's transl)]. Thirteen cases of giant cell arteritis admitted to a Department of Internal Medicine between 1962 and 1977 are reviewed. Giant cell arteritis exhibited different clinical forms, including: temporal arteritis, rheumatic polymyalgia, syndrome of the supra-aortic trunks, cranial arteritis in its ophthalmic and neurologic forms, and generalized arteritis. On the basis of the clinical data, overall examinations, laboratory tests, X-rays, angiograms and histopathologic findings the authors conclude that a single disease is involved. The fact that various of the symptoms occur simultaneously in different patients and that all of the clinical forms have a common pathologic basis points toward giant cell arteritis with different clinical manifestations."}
{"id": "PMID:459590", "title": "[Carcinomatous lymphangitis of the lung. Report of 21 cases (author's transl)].", "content": "Twenty-one cases of carcinomatous lymphangitis of the lung are reported. In diagnosing them clinical, roentgenologic, and histopathologic criteria were used and the clinical, roentgenologic and evolutive findins were considered in order to emphasize the origin of the primitive neoplasm. From this point of view the principal ones turned out to be carcinomas of the breast, stomach, and lung. The absence of carcinoma of the thyroid gland as a source of pulmonary lymphangitis carcinomatosa must be pointed out. The higher incidence of this condition in older people and the clear predominance in the female sex are stressed. From a clinical standpoint the most important data were the existence of cough and dyspnea, which are often associated with the commom presence of enlarged left supraclavicular or axillary lymph nodes. There was a predominant pure interstitial pattern (the most common feature was the presence of Kerley's A or B lines), sometimes coexisting with alveolar impairment and very often with accompanying pleural effusion. The mean survival rate was 2 months, with a maximum of 7 months and a minimum of 3 days. The results of the present series were compared with those of the literature and the most significant and constant fact in both instances was that two thirds of the cases of pulmonary lymphangitis carcinomatosa had their origin in neoplasms of the breast, stomach, and lung.", "contents": "[Carcinomatous lymphangitis of the lung. Report of 21 cases (author's transl)]. Twenty-one cases of carcinomatous lymphangitis of the lung are reported. In diagnosing them clinical, roentgenologic, and histopathologic criteria were used and the clinical, roentgenologic and evolutive findins were considered in order to emphasize the origin of the primitive neoplasm. From this point of view the principal ones turned out to be carcinomas of the breast, stomach, and lung. The absence of carcinoma of the thyroid gland as a source of pulmonary lymphangitis carcinomatosa must be pointed out. The higher incidence of this condition in older people and the clear predominance in the female sex are stressed. From a clinical standpoint the most important data were the existence of cough and dyspnea, which are often associated with the commom presence of enlarged left supraclavicular or axillary lymph nodes. There was a predominant pure interstitial pattern (the most common feature was the presence of Kerley's A or B lines), sometimes coexisting with alveolar impairment and very often with accompanying pleural effusion. The mean survival rate was 2 months, with a maximum of 7 months and a minimum of 3 days. The results of the present series were compared with those of the literature and the most significant and constant fact in both instances was that two thirds of the cases of pulmonary lymphangitis carcinomatosa had their origin in neoplasms of the breast, stomach, and lung."}
{"id": "PMID:459591", "title": "[Early prognosis of acute pancreatitis (author's transl)].", "content": "One hundred patients with acute pancreatitis are studied. The results in 90 cases were \"favorable or very favorable\", in ten cases \"unfavorable or death\". Various different characterisitics were analyzed statistically in relationship to the two types of outcome: sex, clinical histories, and results of physical examination. Furthermore, the individual relationships between age, main initial analytic parameters, and later development were determined. In our experience neither age nor sex, considered individually, showed a significant relationship to the seriousness of the disease. Having had pancreatitis previously proved to be a favorable factor (p less than 0.005). None of the other factors in the case histories showed any bearing of the later course of the condition. Findings in physical examination which were signs of unfavorable prognosis included jaundice (p less than 0.001), low blood pressure (p less than 0.001), tachycardia (p less than 0.005), intestinal paresia (p less than 0.001), pain following decompression (p less than 0.025), and abdominal tenderness (p less than 0.05). Abnormalities in ECG (p less than 0.005), marked leukocytosis (p less than 0.0005), hyperglycemia (p less than 0.02), hypocalcemia (p less than 0.05), and high values for the coefficient of amilase/creatinine clearance (p less than 0.01) also suggested an unfavorable course.", "contents": "[Early prognosis of acute pancreatitis (author's transl)]. One hundred patients with acute pancreatitis are studied. The results in 90 cases were \"favorable or very favorable\", in ten cases \"unfavorable or death\". Various different characterisitics were analyzed statistically in relationship to the two types of outcome: sex, clinical histories, and results of physical examination. Furthermore, the individual relationships between age, main initial analytic parameters, and later development were determined. In our experience neither age nor sex, considered individually, showed a significant relationship to the seriousness of the disease. Having had pancreatitis previously proved to be a favorable factor (p less than 0.005). None of the other factors in the case histories showed any bearing of the later course of the condition. Findings in physical examination which were signs of unfavorable prognosis included jaundice (p less than 0.001), low blood pressure (p less than 0.001), tachycardia (p less than 0.005), intestinal paresia (p less than 0.001), pain following decompression (p less than 0.025), and abdominal tenderness (p less than 0.05). Abnormalities in ECG (p less than 0.005), marked leukocytosis (p less than 0.0005), hyperglycemia (p less than 0.02), hypocalcemia (p less than 0.05), and high values for the coefficient of amilase/creatinine clearance (p less than 0.01) also suggested an unfavorable course."}
{"id": "PMID:459592", "title": "[Study of the causes of death in a hemodialysis unit (author's transl)].", "content": "A review is made of the deaths which occurred in a hemodialysis unit between 1969 and 1977. Sixty-two patients were treated during this period, 18 of whom died. The actuarial percentages of survival are also established. The mortality rate was 15.5 percent during the initial period and 31.5 percent after the fourth year. Deaths in the first monts were caused by cardiac and infectious complications. Those occurring at a later period resulted from the lack of good vascular access which is necessary to maintain a good quality of dialysis.", "contents": "[Study of the causes of death in a hemodialysis unit (author's transl)]. A review is made of the deaths which occurred in a hemodialysis unit between 1969 and 1977. Sixty-two patients were treated during this period, 18 of whom died. The actuarial percentages of survival are also established. The mortality rate was 15.5 percent during the initial period and 31.5 percent after the fourth year. Deaths in the first monts were caused by cardiac and infectious complications. Those occurring at a later period resulted from the lack of good vascular access which is necessary to maintain a good quality of dialysis."}
{"id": "PMID:459593", "title": "[Chronic lymphoid leukemia. Survival in relation to clinical stages. Statistical analysis of 95 cases (author's transl)].", "content": "The prognosis of patients with chronic lymphocytic leukemia is very difficult to evaluate. The classification system by stages, which at the present time is the most simple and useful method for the prognosis of this disease, recognizes five degrees of involvement: stage 0 (medullary and peripheral lymphocytosis); stage I (lymphocytosis + enlarged lymph nodes); stage II (lymphocyosis + hepato-and/or splenomegaly); stage III (lymphocytosis + anemia), and stage IV (lymphocytosis + thrombocytopenia). In the present report 95 controlled patients at the Farreras Valent\u00ed School of Hematology are analyzed using the classification by stages; there were 19 cases in stage 0; 16 in stage I; 30 in stage II; 21 in stage III, and 9 in stage IV. The mean survival rate in the global series was 63 months and the average 90.9 months. As in other series, it was observed that patients in stages 0 and I have a much better prognosis than those in stages III and IV. Stage II occupies an intermediate position in relation to prognosis, since the actuarial survival figure for this stage can practically be superimposed on that of the global series. The classification of chronic lymphocytic leukemia by stages permits the specification of the therapeutic indications for this condition; it also encourages the search for new modalities of treatment for stages with poor prognosis, since in these cases chronic lymphocytic leukemia behaves in the manner of an acute or subacute hemopathy.", "contents": "[Chronic lymphoid leukemia. Survival in relation to clinical stages. Statistical analysis of 95 cases (author's transl)]. The prognosis of patients with chronic lymphocytic leukemia is very difficult to evaluate. The classification system by stages, which at the present time is the most simple and useful method for the prognosis of this disease, recognizes five degrees of involvement: stage 0 (medullary and peripheral lymphocytosis); stage I (lymphocytosis + enlarged lymph nodes); stage II (lymphocyosis + hepato-and/or splenomegaly); stage III (lymphocytosis + anemia), and stage IV (lymphocytosis + thrombocytopenia). In the present report 95 controlled patients at the Farreras Valent\u00ed School of Hematology are analyzed using the classification by stages; there were 19 cases in stage 0; 16 in stage I; 30 in stage II; 21 in stage III, and 9 in stage IV. The mean survival rate in the global series was 63 months and the average 90.9 months. As in other series, it was observed that patients in stages 0 and I have a much better prognosis than those in stages III and IV. Stage II occupies an intermediate position in relation to prognosis, since the actuarial survival figure for this stage can practically be superimposed on that of the global series. The classification of chronic lymphocytic leukemia by stages permits the specification of the therapeutic indications for this condition; it also encourages the search for new modalities of treatment for stages with poor prognosis, since in these cases chronic lymphocytic leukemia behaves in the manner of an acute or subacute hemopathy."}
{"id": "PMID:459594", "title": "[Granulomatous hepatitis. Etiologic study of 107 cases (author's transl)].", "content": "An etiologic study was made of 107 cases of granulomatous hepatitis which were observed in a Department of Internal Medicine between January, 1971 and December, 1977 (excluding the hepatobiliary diseases). The most common etiology was tuberculosis (30 cases, 28 percent) followed by sarcoidosis (19 cases, 17.7 percent), Mediterranean exanthematous fever (13 cases, 12.1 percent), brucellosis (8 cases, 7.4 percent) typhoid fever (7 cases, 6.5 percent) and the idiopathic forms (8 cases, 7.4 percent). A lower rate of incidence was among Hodgkin's disease, toxoplasmosis, adenocarcinomas, leprosy, and those of unknown etiology, classified in this way because the study and follow-up of the patients could not be completed. There were, moreover, individual cases caused by mononucleosis, BCG reaction, hypogammaglobulinemia, celiac disease, and temporal arteritis. From a clinical point of view 50 percent of the patients had hepatomegaly and moderate disturbance of the liver enzymes. The most important enzymatic increases were detected in the cases caused by brucellosis; in the cases which were secondary to sarcoidosis the liver enzymes were normal. A comparison is established between the etiologic incidence of the present series and of others published in the literature. The causes and diagnostic problems of this type of lesion are discussed.", "contents": "[Granulomatous hepatitis. Etiologic study of 107 cases (author's transl)]. An etiologic study was made of 107 cases of granulomatous hepatitis which were observed in a Department of Internal Medicine between January, 1971 and December, 1977 (excluding the hepatobiliary diseases). The most common etiology was tuberculosis (30 cases, 28 percent) followed by sarcoidosis (19 cases, 17.7 percent), Mediterranean exanthematous fever (13 cases, 12.1 percent), brucellosis (8 cases, 7.4 percent) typhoid fever (7 cases, 6.5 percent) and the idiopathic forms (8 cases, 7.4 percent). A lower rate of incidence was among Hodgkin's disease, toxoplasmosis, adenocarcinomas, leprosy, and those of unknown etiology, classified in this way because the study and follow-up of the patients could not be completed. There were, moreover, individual cases caused by mononucleosis, BCG reaction, hypogammaglobulinemia, celiac disease, and temporal arteritis. From a clinical point of view 50 percent of the patients had hepatomegaly and moderate disturbance of the liver enzymes. The most important enzymatic increases were detected in the cases caused by brucellosis; in the cases which were secondary to sarcoidosis the liver enzymes were normal. A comparison is established between the etiologic incidence of the present series and of others published in the literature. The causes and diagnostic problems of this type of lesion are discussed."}
{"id": "PMID:459595", "title": "[Hemodialysis and complement (author's transl)].", "content": "After observing alterations in the activity of the serum complement in patients undergoing periodic hemodialysis, the authors performed a preliminary study to determine repeatedly the activity of C3, C4, and CH50 in 44 patients. They discovered a consistent drop in C3 and CH50 while C4 remained normal. An attempt to explain these findings with information from the literature offered no more than a hypothesis for further study. While the possibility of a decline in the synthesis of the complement factors cannot be disregarded, the authors believe it is much more probable that they are consumed at a rate higher than normal. Since the C4 factor does not appear to be involved, activation is probably along the alternative pathway. Defective synthesis cannot be attributed to liver disease because the latter is not always present and because there is no relationship between C3 levels and levels of albumin or the presence of hepatopathy. The literature was reviewed for data that might support the idea that the complements in these patients are activated continuously by some process in connection with dialysis, by chemical products employed for to clean the machines, by commonly administered drugs, etc. Because so few data could be found on the subject, the authors consider that is necessary to study these mechanisms and their repercussions over a longer period of time.", "contents": "[Hemodialysis and complement (author's transl)]. After observing alterations in the activity of the serum complement in patients undergoing periodic hemodialysis, the authors performed a preliminary study to determine repeatedly the activity of C3, C4, and CH50 in 44 patients. They discovered a consistent drop in C3 and CH50 while C4 remained normal. An attempt to explain these findings with information from the literature offered no more than a hypothesis for further study. While the possibility of a decline in the synthesis of the complement factors cannot be disregarded, the authors believe it is much more probable that they are consumed at a rate higher than normal. Since the C4 factor does not appear to be involved, activation is probably along the alternative pathway. Defective synthesis cannot be attributed to liver disease because the latter is not always present and because there is no relationship between C3 levels and levels of albumin or the presence of hepatopathy. The literature was reviewed for data that might support the idea that the complements in these patients are activated continuously by some process in connection with dialysis, by chemical products employed for to clean the machines, by commonly administered drugs, etc. Because so few data could be found on the subject, the authors consider that is necessary to study these mechanisms and their repercussions over a longer period of time."}
{"id": "PMID:459598", "title": "[Roentgenologic characteristics of Gaucher's disease (author's transl)].", "content": "Gaucher's disease is a rare metabolic disorder in which there is an abnormal accumulation of cerebrosides in the reticuloendothelial system due to a deficit of beta-glucuronidase. Three patients with this disease, 8, 14, and 23 years old, were studied. The purpose of this paper is to point out some of the roentgenologic findings, such as areas of femoral osteolysis, renal venous thrombosis, and in one patient, sclerosis of the sacroiliac joint. Splenomegaly and alteration of the tubular bony structure of the distal end of the femur were present in all three cases. The roentgenologic images of the disease are discussed. The osteoarticular system is the most often affected. Osteolysis is the basic lesion with expansion of the bone marrow and sclerosis. The earliest symptom and sometimes the only one is the Erlenmeyer flask deformity or widening of the distal portion of the femur. Sometimes there is a high degree of deossification without definite osteolytic lesions. Pathologic fractures and fractures caused by pressure of the vertebral bodies are common. Aseptic femoral necrosis are often present. Other less frequent sites of osteoarticular pathology are the skull, jaws, ribs, and sacroiliac joints. Splenomegaly is the most frequent visceral lesion. Other organs sometimes affected are the liver, kidneys, heart, lymphatic system, and lungs, with nodular or basal reticular infiltrations.", "contents": "[Roentgenologic characteristics of Gaucher's disease (author's transl)]. Gaucher's disease is a rare metabolic disorder in which there is an abnormal accumulation of cerebrosides in the reticuloendothelial system due to a deficit of beta-glucuronidase. Three patients with this disease, 8, 14, and 23 years old, were studied. The purpose of this paper is to point out some of the roentgenologic findings, such as areas of femoral osteolysis, renal venous thrombosis, and in one patient, sclerosis of the sacroiliac joint. Splenomegaly and alteration of the tubular bony structure of the distal end of the femur were present in all three cases. The roentgenologic images of the disease are discussed. The osteoarticular system is the most often affected. Osteolysis is the basic lesion with expansion of the bone marrow and sclerosis. The earliest symptom and sometimes the only one is the Erlenmeyer flask deformity or widening of the distal portion of the femur. Sometimes there is a high degree of deossification without definite osteolytic lesions. Pathologic fractures and fractures caused by pressure of the vertebral bodies are common. Aseptic femoral necrosis are often present. Other less frequent sites of osteoarticular pathology are the skull, jaws, ribs, and sacroiliac joints. Splenomegaly is the most frequent visceral lesion. Other organs sometimes affected are the liver, kidneys, heart, lymphatic system, and lungs, with nodular or basal reticular infiltrations."}
{"id": "PMID:459599", "title": "[Liver disease associated with hypernephroma. A case report (author's transl)].", "content": "The observation of a non-metastatic reactive hepatopathy associated with a hypernephroma in a 39-year-old man who had had fever for 4 months led to a review of the literature and an analysis of basically three aspects of the disorder: a) The various manifestations of carcinoma of the kidney, which include a large number of paraneoplastic clinical symptoms (polycythemia, anemia, prolonged fever, hypercalcemia, hypertension, nefropathy, loss of salt, peripheral neuropathy, and amyloidosis); b) an alteracion of hepatic function known since 1961 which is characterized by an abnormal retention of sulfobromophthalein, increase of alkaline phosphatase, prothrombin decrease, dysproteinemia with hypoalbuminemia, and alpha2-globulin increase. It may or may not be accompanied by enlargement of the liver. c) Criteria of operability of the primary tumor.", "contents": "[Liver disease associated with hypernephroma. A case report (author's transl)]. The observation of a non-metastatic reactive hepatopathy associated with a hypernephroma in a 39-year-old man who had had fever for 4 months led to a review of the literature and an analysis of basically three aspects of the disorder: a) The various manifestations of carcinoma of the kidney, which include a large number of paraneoplastic clinical symptoms (polycythemia, anemia, prolonged fever, hypercalcemia, hypertension, nefropathy, loss of salt, peripheral neuropathy, and amyloidosis); b) an alteracion of hepatic function known since 1961 which is characterized by an abnormal retention of sulfobromophthalein, increase of alkaline phosphatase, prothrombin decrease, dysproteinemia with hypoalbuminemia, and alpha2-globulin increase. It may or may not be accompanied by enlargement of the liver. c) Criteria of operability of the primary tumor."}
{"id": "PMID:459600", "title": "[Bile acids. I. Nature, physiology, and functions (author's transl)].", "content": "Bile acids play a fundamental role in the degradation and absorption of intestinal lipids. The primary ones are cholic acid and chenodeoxycholic acid which are synthesized from cholesterol in the liver and conjugate with taurine and glycine amino acids. The secondary bile acids are derived from the primary ones by the enzyme action of intestinal bacteria through a process of deconjugation and dehydroxylation. Their detergent property is based on the molecular configuration of these compounds, which present a hydrophilic and a hydrotion of these compounds, which present a hydrophilic and a hydrophobic surface. The different enzymes in the liver cells that intervene in the process of synthesis of bile acids are now known. A basic element in their physiology is the enterohepatic circulation, enabling the organism to take maximum advantage of these compounds. The dynamics of the cycle are maintained and regulated by the system of uptake and secretion of the cells, cholecystokinin, intestinal peristalsis, active transport across the ileal membrane, and by portal venous flow. Much of our knowledge about the biogenesis and functions of the bile acids has been acquired quite recently. Research over the past three decades has contributed to a great advance in our understanding of their physiology.", "contents": "[Bile acids. I. Nature, physiology, and functions (author's transl)]. Bile acids play a fundamental role in the degradation and absorption of intestinal lipids. The primary ones are cholic acid and chenodeoxycholic acid which are synthesized from cholesterol in the liver and conjugate with taurine and glycine amino acids. The secondary bile acids are derived from the primary ones by the enzyme action of intestinal bacteria through a process of deconjugation and dehydroxylation. Their detergent property is based on the molecular configuration of these compounds, which present a hydrophilic and a hydrotion of these compounds, which present a hydrophilic and a hydrophobic surface. The different enzymes in the liver cells that intervene in the process of synthesis of bile acids are now known. A basic element in their physiology is the enterohepatic circulation, enabling the organism to take maximum advantage of these compounds. The dynamics of the cycle are maintained and regulated by the system of uptake and secretion of the cells, cholecystokinin, intestinal peristalsis, active transport across the ileal membrane, and by portal venous flow. Much of our knowledge about the biogenesis and functions of the bile acids has been acquired quite recently. Research over the past three decades has contributed to a great advance in our understanding of their physiology."}
{"id": "PMID:459601", "title": "[Platelet function in systemic lupus erythematosus (author's transl)].", "content": "The platelet function was studied in 23 patients with systemic lupus erythematosus, all of whom met the diagnostic creteria established by the American Rheumatism Association. They were not under any treatment, especially with any drug that might interfere with platelet function. The same study was performed on a control group composed of volunteers donors at a blood bank. The platelet count was definitely lower in the patients with lupus than in the control subjects (p less than 0.0005), although a clear thrombopenia was observed in only two indivduals (8.7 percent). Anti-platelet antibodies were found in only six cases (26 percent). There was a linear correlation between thrombopenia and the presence of hemorrhagic diathesis and low levels of C4 and CH50 components. Plateler adhesiveness was clearly lower in the lupus group than in the control group (p less than 0.0005). The presence of kidney disease determined a greater impairment of the platelet adhesiveness (p less than 0.0025). A notable defect on platelet aggregation was induced by ADP, adrenaline and collagen. This was more apparent in the group of patients exhibiting a higher degree of clinical activity and in those who showed a serum complement decrease. The mechanism responsible for this thrombopathy appears to be an interference in the platelet function due to the presence of circulating immunocomplexes. They adhere to the platelet membrane blocking its function and inhibiting the release of the necessary thrombocytic components for the second phase of the aggregation. This platelet alteration is not usually manifested clinically; for this reason no relationship was found between this platelet defect and the presence of hemorrhagic symptoms in our patients. The condition is reversible and may disappear after therapy with steroids and/or immunosuppresive agents.", "contents": "[Platelet function in systemic lupus erythematosus (author's transl)]. The platelet function was studied in 23 patients with systemic lupus erythematosus, all of whom met the diagnostic creteria established by the American Rheumatism Association. They were not under any treatment, especially with any drug that might interfere with platelet function. The same study was performed on a control group composed of volunteers donors at a blood bank. The platelet count was definitely lower in the patients with lupus than in the control subjects (p less than 0.0005), although a clear thrombopenia was observed in only two indivduals (8.7 percent). Anti-platelet antibodies were found in only six cases (26 percent). There was a linear correlation between thrombopenia and the presence of hemorrhagic diathesis and low levels of C4 and CH50 components. Plateler adhesiveness was clearly lower in the lupus group than in the control group (p less than 0.0005). The presence of kidney disease determined a greater impairment of the platelet adhesiveness (p less than 0.0025). A notable defect on platelet aggregation was induced by ADP, adrenaline and collagen. This was more apparent in the group of patients exhibiting a higher degree of clinical activity and in those who showed a serum complement decrease. The mechanism responsible for this thrombopathy appears to be an interference in the platelet function due to the presence of circulating immunocomplexes. They adhere to the platelet membrane blocking its function and inhibiting the release of the necessary thrombocytic components for the second phase of the aggregation. This platelet alteration is not usually manifested clinically; for this reason no relationship was found between this platelet defect and the presence of hemorrhagic symptoms in our patients. The condition is reversible and may disappear after therapy with steroids and/or immunosuppresive agents."}
{"id": "PMID:459602", "title": "[Epidemiologic data on bronchogenic carcinoma (author's transl)].", "content": "The Bronchogenic Carcinoma Co-operative Group analyzed the epidemiologic data of 240 cases of bronchogenic carcinoma in a prospective study. When risk factors (exposure to potencial pathogenic substances) were studied, a relationship was noted between the incidence at an early age and the presence of nondifferentiated small cell carcinoma. The number of years of exposure to tobacco had a direct effect on the curative value of the thoracotomy and the survival rate. Forty years of smoking appeared to be borderline for a poor prognosis. Localization of the tumor by bronchoscopy showed a frequency in the left main bronchus (18 percent) more than twice than in the right (8 percent). In 25 percent of the cases the tumor was more peripheral than the subsegmental situation according to the bronchofiberoptic examination. Evaluation of clinical data only showed a lack of correlation between the different symptoms and the various means of evaluation and the prognosis of the disease. For example, a patient with hemoptysis has a statistically higher probability of having an epidermoid tumor and a greater possibility of a successful curative thoracotomy (23 percent as opposed to 17 percent for the rest). The group of incidental cases (9 percent of the total) was analyzed. The degree of resectability was higher as compared with the rest (62 percent against 34 percent) as well as the absence of nondifferentiated small cell type carcinomas.", "contents": "[Epidemiologic data on bronchogenic carcinoma (author's transl)]. The Bronchogenic Carcinoma Co-operative Group analyzed the epidemiologic data of 240 cases of bronchogenic carcinoma in a prospective study. When risk factors (exposure to potencial pathogenic substances) were studied, a relationship was noted between the incidence at an early age and the presence of nondifferentiated small cell carcinoma. The number of years of exposure to tobacco had a direct effect on the curative value of the thoracotomy and the survival rate. Forty years of smoking appeared to be borderline for a poor prognosis. Localization of the tumor by bronchoscopy showed a frequency in the left main bronchus (18 percent) more than twice than in the right (8 percent). In 25 percent of the cases the tumor was more peripheral than the subsegmental situation according to the bronchofiberoptic examination. Evaluation of clinical data only showed a lack of correlation between the different symptoms and the various means of evaluation and the prognosis of the disease. For example, a patient with hemoptysis has a statistically higher probability of having an epidermoid tumor and a greater possibility of a successful curative thoracotomy (23 percent as opposed to 17 percent for the rest). The group of incidental cases (9 percent of the total) was analyzed. The degree of resectability was higher as compared with the rest (62 percent against 34 percent) as well as the absence of nondifferentiated small cell type carcinomas."}
{"id": "PMID:459603", "title": "[Role of secondary hyperaldosteronism in the pathomecanism of hydropic decompensation in the cirrhotic patient (author's transl)].", "content": "Plasma aldosterone levels before and after walking were compared in a series of 10 controls and 41 patients with cirrhosis of the liver. The latter were distributed in the following way: 8 had compensated cirrhosis, the remaining 33 were in a situaci\u00f3n of hydropic decompensation, 10 with associated renal insufficiency, and 23 without. Basal aldosterone levels in compensated cirrhotics were similar to those of the controls, but these values increased significantly more than the controls following postural stimulation. Decompensated cirrhotics without renal insufficiency had significantly higher values than the controls, both in basal conditions and after stimulation. The highest values corresponded to the decompensated cirrhotic patients with renal insufficiency who were in advanced stages of liver disease. On the basis of the present findings and those of other authors, it is suggested that a certain reduction in the metabolic clearance of aldosterone appears to exist in hepatic cirrhosis. However, hormonal hyperproduction is the dominant factor in the pathogenic mechanism of secondary hyperaldosteronism. The pathogenesis of the excessive production of hormone is discussed. In conclusion, it appears that the scant affluence of sodium to the macula densa may be the primary factor in explaining this common situation in patients with cirrhosis of the liver.", "contents": "[Role of secondary hyperaldosteronism in the pathomecanism of hydropic decompensation in the cirrhotic patient (author's transl)]. Plasma aldosterone levels before and after walking were compared in a series of 10 controls and 41 patients with cirrhosis of the liver. The latter were distributed in the following way: 8 had compensated cirrhosis, the remaining 33 were in a situaci\u00f3n of hydropic decompensation, 10 with associated renal insufficiency, and 23 without. Basal aldosterone levels in compensated cirrhotics were similar to those of the controls, but these values increased significantly more than the controls following postural stimulation. Decompensated cirrhotics without renal insufficiency had significantly higher values than the controls, both in basal conditions and after stimulation. The highest values corresponded to the decompensated cirrhotic patients with renal insufficiency who were in advanced stages of liver disease. On the basis of the present findings and those of other authors, it is suggested that a certain reduction in the metabolic clearance of aldosterone appears to exist in hepatic cirrhosis. However, hormonal hyperproduction is the dominant factor in the pathogenic mechanism of secondary hyperaldosteronism. The pathogenesis of the excessive production of hormone is discussed. In conclusion, it appears that the scant affluence of sodium to the macula densa may be the primary factor in explaining this common situation in patients with cirrhosis of the liver."}
{"id": "PMID:459604", "title": "[Non-secretory myeloma. Report of a case with negative immunofluorescence in the tumor cells (author's transl)].", "content": "A 39-year-old patient with bilateral paralysis of the VI cranial nerve was studied. Numerous foci of intracranial osteolysis were discovered as well as on the right calvicle and several ribs. Two bone marrow aspirates gave results within normal limits. A biopsy of the lesion on the right calvicle led to the diagnosis of myelomatosis. Immunoelectrophoresis showed that paraprotein was not present in serum and urine. Immunofluorescence with antisera marked with fluorescein was negative in the diseased bone marrow. Treatment with melphalan and prednisone given alternately produced an improvement in all of the lytic lesions within several months; 20 months after treatment was started no new destructive bony lesions have appeared. There have been very few reported cases of non-secretory myeloma. In most of these cases the presence of immunoglobulins in the tumoral cells has been shown by immunofluorescence. The absence of the immunoglobulins, as described in our case, is extremely rare. Unlike most cases of non-secretory myeloma published to date, our patient has a favorable clinical course after treatment was administered.", "contents": "[Non-secretory myeloma. Report of a case with negative immunofluorescence in the tumor cells (author's transl)]. A 39-year-old patient with bilateral paralysis of the VI cranial nerve was studied. Numerous foci of intracranial osteolysis were discovered as well as on the right calvicle and several ribs. Two bone marrow aspirates gave results within normal limits. A biopsy of the lesion on the right calvicle led to the diagnosis of myelomatosis. Immunoelectrophoresis showed that paraprotein was not present in serum and urine. Immunofluorescence with antisera marked with fluorescein was negative in the diseased bone marrow. Treatment with melphalan and prednisone given alternately produced an improvement in all of the lytic lesions within several months; 20 months after treatment was started no new destructive bony lesions have appeared. There have been very few reported cases of non-secretory myeloma. In most of these cases the presence of immunoglobulins in the tumoral cells has been shown by immunofluorescence. The absence of the immunoglobulins, as described in our case, is extremely rare. Unlike most cases of non-secretory myeloma published to date, our patient has a favorable clinical course after treatment was administered."}
{"id": "PMID:459605", "title": "[Sarcoidosis, toxic thyroid adenoma, and De Quervain's thyroiditis. Association or coincidence (author's transl)].", "content": "The case of an association of three well-differentiated pathologies is reported. A 40-year-old woman with a toxic thyroid adenoma developed acute polyarthritis during the course of the disease. The articular symptoms together with chest X-rays and a consistently negative PPD (1:100) were suggestive of sarcoidosis. This was later confirmed by the discovery of non-caseating granulomas in the liver and superficial lymph nodes, and by a positive Kveim's test. Histopathologic examination of the thyroid gland confirmed the existence of the adenoma. Sarcoid infiltration was not observed, but on the other hand the typical images of subacute granulomatous thyroiditis (De Quervain's thyroiditis) were discovered.", "contents": "[Sarcoidosis, toxic thyroid adenoma, and De Quervain's thyroiditis. Association or coincidence (author's transl)]. The case of an association of three well-differentiated pathologies is reported. A 40-year-old woman with a toxic thyroid adenoma developed acute polyarthritis during the course of the disease. The articular symptoms together with chest X-rays and a consistently negative PPD (1:100) were suggestive of sarcoidosis. This was later confirmed by the discovery of non-caseating granulomas in the liver and superficial lymph nodes, and by a positive Kveim's test. Histopathologic examination of the thyroid gland confirmed the existence of the adenoma. Sarcoid infiltration was not observed, but on the other hand the typical images of subacute granulomatous thyroiditis (De Quervain's thyroiditis) were discovered."}
{"id": "PMID:459606", "title": "[Viral etiology of insulin-dependent diabetes (author's transl)].", "content": "Data are collected here that may support the hypothesis that there is an infectious component in the genesis of insulin-dependent diabetes mellitus. The clinical and experimental data on which that hypothesis is based referring to viruses such as: mumps virus, rubella virus, encephalomyocarditis virus, and Coxsackie B viruses, are presented. The authors mention the pathogenic mechanism, which extends over the existence of diabetogenic genes linked to the major complex of the histocompatibility HLA system, as well as the existence of an autoimmune reaction induced by the infecting virus. This review shows up the large gaps that exist in the accurate knowledge we have about the possible infectious etiology of diabetes. However, it also offers different pathogenic possibilities for further experimental investigations on bases that are reasonably scientifically consistent.", "contents": "[Viral etiology of insulin-dependent diabetes (author's transl)]. Data are collected here that may support the hypothesis that there is an infectious component in the genesis of insulin-dependent diabetes mellitus. The clinical and experimental data on which that hypothesis is based referring to viruses such as: mumps virus, rubella virus, encephalomyocarditis virus, and Coxsackie B viruses, are presented. The authors mention the pathogenic mechanism, which extends over the existence of diabetogenic genes linked to the major complex of the histocompatibility HLA system, as well as the existence of an autoimmune reaction induced by the infecting virus. This review shows up the large gaps that exist in the accurate knowledge we have about the possible infectious etiology of diabetes. However, it also offers different pathogenic possibilities for further experimental investigations on bases that are reasonably scientifically consistent."}
{"id": "PMID:459610", "title": "A simple data-processing system for the monitoring of cross-infection in a district general hospital.", "content": "A year's trail has demonstrated the effectiveness of a simple data processing system in the early identification of cross-infection problems at their source. The system has particular advantanges for small and scattered surgical units where cross-infection monitoring is particularly difficult because patients are transferred before their treatment is complete. A simple extension of the system could audit the efficiency of preventive antiseptic measures, particularly aseptic techniques, and provide antibiotic profiles.", "contents": "A simple data-processing system for the monitoring of cross-infection in a district general hospital. A year's trail has demonstrated the effectiveness of a simple data processing system in the early identification of cross-infection problems at their source. The system has particular advantanges for small and scattered surgical units where cross-infection monitoring is particularly difficult because patients are transferred before their treatment is complete. A simple extension of the system could audit the efficiency of preventive antiseptic measures, particularly aseptic techniques, and provide antibiotic profiles."}
{"id": "PMID:459612", "title": "History and future of the MUMPS in medical computing.", "content": "MUMPS is an integrated language and operating system. It was originally developed in the mid 60s, primarily for the interactive manipulation of text-like, hierachical data found so pervasively in medicine. This paper traces the growth in use of MUMPS. Some of the many medical application for which MUMPS is used are discussed, as are the achievements and goals of the MUMPS Users' Group, particularly in facilitating application transfer. The influence of federal funding upon the growth and use of MUMPS is noted. Of particular importance is the federal support of the MUMPS Development Committee in the standardization of MUMPS, and the MUMPS User's Group in promoting MUMPS information and application exchange.", "contents": "History and future of the MUMPS in medical computing. MUMPS is an integrated language and operating system. It was originally developed in the mid 60s, primarily for the interactive manipulation of text-like, hierachical data found so pervasively in medicine. This paper traces the growth in use of MUMPS. Some of the many medical application for which MUMPS is used are discussed, as are the achievements and goals of the MUMPS Users' Group, particularly in facilitating application transfer. The influence of federal funding upon the growth and use of MUMPS is noted. Of particular importance is the federal support of the MUMPS Development Committee in the standardization of MUMPS, and the MUMPS User's Group in promoting MUMPS information and application exchange."}
{"id": "PMID:459611", "title": "Analysis of the oxygen supply to porous implants in bone.", "content": "A mathematical model of cylindrical porous surgical implant for orthopaedic prosthesis is presented. The model allows calculation of the net oxygen consumption as a function of porosity and pore diameter from 0 to 500 microM. In addition, limitations on implant size and pore diameter due to the length of the arteriole 'tree' have been considered. Literature data have been used to evaluate the model of the implant when infiltrated with bone in the steady-state. The model suggests that minimum pore diameter of 75 microM is necessary for bone ingrowth. Further, the limiting relationship between pellet diameter and pore diameter due to arterial tree length has been found to be a nearly linear function, with slope dependent on the tortuosity of the pores of the implant.", "contents": "Analysis of the oxygen supply to porous implants in bone. A mathematical model of cylindrical porous surgical implant for orthopaedic prosthesis is presented. The model allows calculation of the net oxygen consumption as a function of porosity and pore diameter from 0 to 500 microM. In addition, limitations on implant size and pore diameter due to the length of the arteriole 'tree' have been considered. Literature data have been used to evaluate the model of the implant when infiltrated with bone in the steady-state. The model suggests that minimum pore diameter of 75 microM is necessary for bone ingrowth. Further, the limiting relationship between pellet diameter and pore diameter due to arterial tree length has been found to be a nearly linear function, with slope dependent on the tortuosity of the pores of the implant."}
{"id": "PMID:459613", "title": "MUMPS applications in doctor's office systems.", "content": "As basic elements of an information network in ambulatory care, doctor's office computers are currently being tested in various hardward and software configurations. MUMPS has proved to be most effective in developing complex text-oriented application packages on small-scale computers. Separation of program and text modules increased the flexibiltiy necessary to serve different medical specialities. The final goal of the project is improved economic and organizational efficiency in the management of ambulatory care. The field test is supported by the German government (DOMINIG DVM 404).", "contents": "MUMPS applications in doctor's office systems. As basic elements of an information network in ambulatory care, doctor's office computers are currently being tested in various hardward and software configurations. MUMPS has proved to be most effective in developing complex text-oriented application packages on small-scale computers. Separation of program and text modules increased the flexibiltiy necessary to serve different medical specialities. The final goal of the project is improved economic and organizational efficiency in the management of ambulatory care. The field test is supported by the German government (DOMINIG DVM 404)."}
{"id": "PMID:459644", "title": "[Symptomatology and treatment of malformations of the inferior vena cava (author's transl)].", "content": "A review of the embryology, pathophysiology semiotics and surgical treatment of malformations of the inferior vena cava is given based on 3 clinical histories. The cases consisted of a typical membrane occlusion, an atresia of the infrarenal segment and of a hitherto not described combination of several malformations of the inferior vena cava: membrane occlusion, aplasia of the infrarenal segment of the vena cava inferior and multiple aneurysms of the iliac vein. The symptomatology of malformations of the vena cava inferior depends on the compensation by the collateral or the persisting embryonic veins, respectively, and on the localization and the degree of the obliteration. The varying hemodynamic reactions are described. The following surgical methods for the treatment of inferior vena cava occlusions in the hepatic segment are available: The so called conservative interventions for the creation of collaterals, the direct or indirect recanalization and the bypass operation. Congenital infrarenal atresias of the vena cava are corrected by homologous or prosthetic interposition. The venous replacement with a bovine heterograft presented here, has not yet been described in the literature.", "contents": "[Symptomatology and treatment of malformations of the inferior vena cava (author's transl)]. A review of the embryology, pathophysiology semiotics and surgical treatment of malformations of the inferior vena cava is given based on 3 clinical histories. The cases consisted of a typical membrane occlusion, an atresia of the infrarenal segment and of a hitherto not described combination of several malformations of the inferior vena cava: membrane occlusion, aplasia of the infrarenal segment of the vena cava inferior and multiple aneurysms of the iliac vein. The symptomatology of malformations of the vena cava inferior depends on the compensation by the collateral or the persisting embryonic veins, respectively, and on the localization and the degree of the obliteration. The varying hemodynamic reactions are described. The following surgical methods for the treatment of inferior vena cava occlusions in the hepatic segment are available: The so called conservative interventions for the creation of collaterals, the direct or indirect recanalization and the bypass operation. Congenital infrarenal atresias of the vena cava are corrected by homologous or prosthetic interposition. The venous replacement with a bovine heterograft presented here, has not yet been described in the literature."}
{"id": "PMID:459645", "title": "[Experiences in 130 operations of carotid elongation (author's transl)].", "content": "A report on 130 operations of carotid elongation is presented. Indications for reconstruction are based on angiography, neurologic symptoms, and recently, on ultrasonography (Doppler's). Several surgical methods have been performed: 25 resections of the internal carotid artery, 5 resections of the common carotid artery, 3 resections of loop aneurysms. The main operative techniques were resection and neoimplantation, the latter with patch-plastic surgery. Usually, a shunt was used. Mortality was 0%, lethality, 2%. During a follow-up period of 6 weeks to 2 years, 86% of the patients were found to be free of complaints, and 14% were without change of the neurologic symptoms. The good neurologic results are convincing.", "contents": "[Experiences in 130 operations of carotid elongation (author's transl)]. A report on 130 operations of carotid elongation is presented. Indications for reconstruction are based on angiography, neurologic symptoms, and recently, on ultrasonography (Doppler's). Several surgical methods have been performed: 25 resections of the internal carotid artery, 5 resections of the common carotid artery, 3 resections of loop aneurysms. The main operative techniques were resection and neoimplantation, the latter with patch-plastic surgery. Usually, a shunt was used. Mortality was 0%, lethality, 2%. During a follow-up period of 6 weeks to 2 years, 86% of the patients were found to be free of complaints, and 14% were without change of the neurologic symptoms. The good neurologic results are convincing."}
{"id": "PMID:459646", "title": "[Surgery of bronchus carcinoma (author's transl)].", "content": "The results of surgery for bronchial carcinoma can be improved by further stressing of early diagnosis, by operative methods adapted to oncological needs and assessment of techniques which increase operability as well as by adequate and general additional therapy. Based on our material of 8,423 patients of which 2,773 (i.e. 23%) have been operated the results of the operation in relation to type of operation, histology, Feinstein's classification, TNM stages and other are demonstrated.", "contents": "[Surgery of bronchus carcinoma (author's transl)]. The results of surgery for bronchial carcinoma can be improved by further stressing of early diagnosis, by operative methods adapted to oncological needs and assessment of techniques which increase operability as well as by adequate and general additional therapy. Based on our material of 8,423 patients of which 2,773 (i.e. 23%) have been operated the results of the operation in relation to type of operation, histology, Feinstein's classification, TNM stages and other are demonstrated."}
{"id": "PMID:459647", "title": "[Reconstructive procedure in aortoiliofemoral occlusive disease (author's transl)].", "content": "Based on experience with about 1,000 patients with operable aortoiliofemoral occlusive disease, the factors influencing the operative procedure are discussed. These include the angiographic picture, the severity of ischemia, general risks, accessibility of the operating field, and availability of reconstructive material. It is concluded that, in discussing the operative procedure in aortoiliofemoral occlusive disease, the central question must be, which procedure is best for this patient, in view of his special circumstances, and not, which procedure is technically possible.", "contents": "[Reconstructive procedure in aortoiliofemoral occlusive disease (author's transl)]. Based on experience with about 1,000 patients with operable aortoiliofemoral occlusive disease, the factors influencing the operative procedure are discussed. These include the angiographic picture, the severity of ischemia, general risks, accessibility of the operating field, and availability of reconstructive material. It is concluded that, in discussing the operative procedure in aortoiliofemoral occlusive disease, the central question must be, which procedure is best for this patient, in view of his special circumstances, and not, which procedure is technically possible."}
{"id": "PMID:459648", "title": "[The distal spleno-renal anastomosis of Warren (author's transl)].", "content": "This is a report on 28 Warren-Shunts, 17 of these patients had undergone clinical, hematological and angiographic controls 2--46 (mean 10) months after the operation. The critical evaluation of the results shows that the Warren derivation, which is selective at the moment of operation, develops in the following time some hepatofugal circulation. This is a more radical effect than the intended drainage of the gastro-esophageal venous bed alone.", "contents": "[The distal spleno-renal anastomosis of Warren (author's transl)]. This is a report on 28 Warren-Shunts, 17 of these patients had undergone clinical, hematological and angiographic controls 2--46 (mean 10) months after the operation. The critical evaluation of the results shows that the Warren derivation, which is selective at the moment of operation, develops in the following time some hepatofugal circulation. This is a more radical effect than the intended drainage of the gastro-esophageal venous bed alone."}
{"id": "PMID:459650", "title": "[Cumulometry with fast components of nystagmus (author's transl)].", "content": "The cumulogram of slow nystagmus phases has proved as a useful method for the analysis of vestibular nystagmus. However, its computation depends on the availability of special apparatuses. Therefore, the relations are studied between the cumulogram of slow phases and the cumulogram of fast phases that can be determined more easily. The analysis of 57 pendular tests shows the close correlation (r = 0,98) of the respective values for vestibular gain. Considering the statistical connection the comparison of these two methods and their results, resp., is well practicable.", "contents": "[Cumulometry with fast components of nystagmus (author's transl)]. The cumulogram of slow nystagmus phases has proved as a useful method for the analysis of vestibular nystagmus. However, its computation depends on the availability of special apparatuses. Therefore, the relations are studied between the cumulogram of slow phases and the cumulogram of fast phases that can be determined more easily. The analysis of 57 pendular tests shows the close correlation (r = 0,98) of the respective values for vestibular gain. Considering the statistical connection the comparison of these two methods and their results, resp., is well practicable."}
{"id": "PMID:459651", "title": "[Malignant fly-grub infection (myiasis) of the ear (author's transl)].", "content": "On the basis of an own observation of a malignant fly-grub infection of the ear and by means of a detailed checking of the literature a benignant and a malignant shape of this disease are distinguished. The peculiarities of the mode of infection in relation to the fly-species are described, some apparent typical characteristics of the Muscidae-Larva infection are put out and directions for treatment are given. The disease nearly wrotten in Europe to-day was not a seldom event especially in the eastern parts of Germany.", "contents": "[Malignant fly-grub infection (myiasis) of the ear (author's transl)]. On the basis of an own observation of a malignant fly-grub infection of the ear and by means of a detailed checking of the literature a benignant and a malignant shape of this disease are distinguished. The peculiarities of the mode of infection in relation to the fly-species are described, some apparent typical characteristics of the Muscidae-Larva infection are put out and directions for treatment are given. The disease nearly wrotten in Europe to-day was not a seldom event especially in the eastern parts of Germany."}
{"id": "PMID:459652", "title": "[Sarcoidosis of the larynx (author's transl)].", "content": "The isolated case of sarcoidosis of the larynx is very rare. We are referred to a case of histologically confirmed by the kweim-test of sarcoidosis of the false vocal cord. The problems of the case are discussed.", "contents": "[Sarcoidosis of the larynx (author's transl)]. The isolated case of sarcoidosis of the larynx is very rare. We are referred to a case of histologically confirmed by the kweim-test of sarcoidosis of the false vocal cord. The problems of the case are discussed."}
{"id": "PMID:459653", "title": "Malignant melanoma of the scalp.", "content": "Malignant melanoma of the scalp has a significantly worse prognosis than cutaneous melanoma arising in other head and neck sites. In this series, 125 patients were treated for Stage I invasive melanoma of the scalp and followed 3 to 19 years. Survival rates for these patients were calculated on the basis of several factors. Survival after treatment was not affected by the age and sex of the patient, size and site of the primary, or treatment of the primary lesion, although local failure was higher among those treated by primary excision and closure. Patients undergoing elective neck dissection with histologically negative nodes had significantly better survival rates than those with histologically positive nodes or patients in whom a neck dissection was not performed.", "contents": "Malignant melanoma of the scalp. Malignant melanoma of the scalp has a significantly worse prognosis than cutaneous melanoma arising in other head and neck sites. In this series, 125 patients were treated for Stage I invasive melanoma of the scalp and followed 3 to 19 years. Survival rates for these patients were calculated on the basis of several factors. Survival after treatment was not affected by the age and sex of the patient, size and site of the primary, or treatment of the primary lesion, although local failure was higher among those treated by primary excision and closure. Patients undergoing elective neck dissection with histologically negative nodes had significantly better survival rates than those with histologically positive nodes or patients in whom a neck dissection was not performed."}
{"id": "PMID:459654", "title": "Long-term follow-up of tympanic neurectomy for sialorrhea.", "content": "Thirty-one patients who had undergone a bilateral tympanic neurectomy (sectioning of both Jacobson's nerve and the chorda tympani) for sialorrhea are evaluated after two years (ranging from 24--45 months). Drooling control improved in 74%. There was a significant difference of improvement in the group where a diligent search was made to sever all branches of the tympanic plexus. A 50% improvement was attained in two failures of a tympanic neurectomy who underwent transposition of Stenson's ducts into the tonsillar fossa.", "contents": "Long-term follow-up of tympanic neurectomy for sialorrhea. Thirty-one patients who had undergone a bilateral tympanic neurectomy (sectioning of both Jacobson's nerve and the chorda tympani) for sialorrhea are evaluated after two years (ranging from 24--45 months). Drooling control improved in 74%. There was a significant difference of improvement in the group where a diligent search was made to sever all branches of the tympanic plexus. A 50% improvement was attained in two failures of a tympanic neurectomy who underwent transposition of Stenson's ducts into the tonsillar fossa."}
{"id": "PMID:459655", "title": "Amyloidosis presenting as a mass in the neck.", "content": "A 54-year-old white male presented with a large suprahyoid midline mass. On thorough history and physical examination, he had features which characterize amyloidosis, including congestive heart failure and carpal tunnel syndrome. The classification, clinical findings, and associated syndromes are reviewed. A striking feature of the amyloid deposit is its rapid growth. Amyloidosis should now be considered in a differential diagnosis of a neck mass.", "contents": "Amyloidosis presenting as a mass in the neck. A 54-year-old white male presented with a large suprahyoid midline mass. On thorough history and physical examination, he had features which characterize amyloidosis, including congestive heart failure and carpal tunnel syndrome. The classification, clinical findings, and associated syndromes are reviewed. A striking feature of the amyloid deposit is its rapid growth. Amyloidosis should now be considered in a differential diagnosis of a neck mass."}
{"id": "PMID:459656", "title": "Diatrizoate meglumine (Hypaque) treatment for sudden hearing loss.", "content": "During the 24 month period ending in December 1978, 31 patients with sudden idiopathic sensorineural hearing loss were treated with vasodilators and Hypaque. Fifteen patients met the treatment criteria of being treated within the first month following onset, no vertigo, and the hearing loss was not complete. Twelve patients (80%) had complete return of their hearing. Two patients (13%) had partial return of their hearing, and 1 patient (6%) had no response to treatment. Of the 16 patients treated who did not meet the criteria, 6 patients (38%) had complete return of their hearing. Eight patients (50%) had partial return of their hearing and 2 patients (12%) had no return of their hearing.", "contents": "Diatrizoate meglumine (Hypaque) treatment for sudden hearing loss. During the 24 month period ending in December 1978, 31 patients with sudden idiopathic sensorineural hearing loss were treated with vasodilators and Hypaque. Fifteen patients met the treatment criteria of being treated within the first month following onset, no vertigo, and the hearing loss was not complete. Twelve patients (80%) had complete return of their hearing. Two patients (13%) had partial return of their hearing, and 1 patient (6%) had no response to treatment. Of the 16 patients treated who did not meet the criteria, 6 patients (38%) had complete return of their hearing. Eight patients (50%) had partial return of their hearing and 2 patients (12%) had no return of their hearing."}
{"id": "PMID:459657", "title": "Metastatic tumors to the sphenoid sinus.", "content": "Patients with cranial nerve palsies and roentgenographic evidence of sphenoid sinus destruction present diagnostic problems. Although the presence of local primary neoplasms may be considered first, metastatic disease from distant sites also should be considered. We report on eight patients with metastatic tumors to the sphenoid sinus seen at the Mayo Clinic from 1950 through 1976. Primary sites of the lesions included the breast, thyroid gland, lung, kidney, and prostate (two patients). Metastatic myeloma was seen in two patients. Symptoms resulted from involvement of the structures adjacent to the sphenoid sinus and in all patients included diplopia or decreased visual acuity. In four patients, these ocular symptoms were the first sign of disease, after which a complete general examination disclosed the occult primary sites.", "contents": "Metastatic tumors to the sphenoid sinus. Patients with cranial nerve palsies and roentgenographic evidence of sphenoid sinus destruction present diagnostic problems. Although the presence of local primary neoplasms may be considered first, metastatic disease from distant sites also should be considered. We report on eight patients with metastatic tumors to the sphenoid sinus seen at the Mayo Clinic from 1950 through 1976. Primary sites of the lesions included the breast, thyroid gland, lung, kidney, and prostate (two patients). Metastatic myeloma was seen in two patients. Symptoms resulted from involvement of the structures adjacent to the sphenoid sinus and in all patients included diplopia or decreased visual acuity. In four patients, these ocular symptoms were the first sign of disease, after which a complete general examination disclosed the occult primary sites."}
{"id": "PMID:459659", "title": "Gunshot injury to the temporal bone: an analysis of thirty-five cases.", "content": "By retrospective analysis of 35 surviving gunshot injuries to the temporal bone and by presentation of 6 representative cases, the management and reconstructive procedures of these injuries in the Tulane University affiliated hospitals are presented. The most frequent single injury was facial nerve paralysis (16 cases), followed by external canal injury and conductive hearing loss. Anacusis occurred in 12 cases. In conductive hearing loss the intact posterior wall tympanoplasty with mastoidectomy (16 cases), or the modified radical mastoidectomy (5 cases), allowed middle ear reconstruction. Transmastoid facial canal decompression, combined with the above procedures or with a middle fossa craniotomy, was performed in 6 cases. Delayed facial reconstruction (5 cases) utilized microneural anastomosis between facial-facial and hypoglossal-facial nerves and fascia lata slings. Intracranial complications of thrombosis of internal carotid artery, dural venous lacerations, temporal lobe aphasia, bitemporal hemianopsia, cerebral abscesses and meningitis are also discussed.", "contents": "Gunshot injury to the temporal bone: an analysis of thirty-five cases. By retrospective analysis of 35 surviving gunshot injuries to the temporal bone and by presentation of 6 representative cases, the management and reconstructive procedures of these injuries in the Tulane University affiliated hospitals are presented. The most frequent single injury was facial nerve paralysis (16 cases), followed by external canal injury and conductive hearing loss. Anacusis occurred in 12 cases. In conductive hearing loss the intact posterior wall tympanoplasty with mastoidectomy (16 cases), or the modified radical mastoidectomy (5 cases), allowed middle ear reconstruction. Transmastoid facial canal decompression, combined with the above procedures or with a middle fossa craniotomy, was performed in 6 cases. Delayed facial reconstruction (5 cases) utilized microneural anastomosis between facial-facial and hypoglossal-facial nerves and fascia lata slings. Intracranial complications of thrombosis of internal carotid artery, dural venous lacerations, temporal lobe aphasia, bitemporal hemianopsia, cerebral abscesses and meningitis are also discussed."}
{"id": "PMID:459663", "title": "Alveolar soft part sarcoma of the head and neck.", "content": "The twelfth case (in 25 years of literature) of alveolar soft part sarcoma of the head and neck is presented. It represents the seventh case to occur in the base of tongue and the second to be associated with pregnancy. The average age of onset is in the second decade with a 2:1 female preponderance and a marked tendency to right-sided occurrence. Treatment with surgery and/or radiotherapy is not curative. Local recurrence is common as are distant metastases, usually to lung, brain or bone. Theoretically, chemotherapy may offer better control of disease but few agents have shown dramatic response. The characteristics of the tumors are discussed and various chemotherapeutic agents are reviewed.", "contents": "Alveolar soft part sarcoma of the head and neck. The twelfth case (in 25 years of literature) of alveolar soft part sarcoma of the head and neck is presented. It represents the seventh case to occur in the base of tongue and the second to be associated with pregnancy. The average age of onset is in the second decade with a 2:1 female preponderance and a marked tendency to right-sided occurrence. Treatment with surgery and/or radiotherapy is not curative. Local recurrence is common as are distant metastases, usually to lung, brain or bone. Theoretically, chemotherapy may offer better control of disease but few agents have shown dramatic response. The characteristics of the tumors are discussed and various chemotherapeutic agents are reviewed."}
{"id": "PMID:459664", "title": "Laryngotracheal trauma: observations on its pathogenesis and its prevention following prolonged orotracheal intubation in the adult.", "content": "The authors have clinically examined the larynges of 117 patients who were successfully extubated in a Respiratory Care Unit, and have examined the larynges at autopsy from an additional 68 patients who failed to survive in the same Respiratory Care Unit. Based on these observations, our understanding of the pathogenesis of laryngotracheal trauma has allowed us to modify our clinical management in order to minimize such damage. Influence of this study on the management of patients is described.", "contents": "Laryngotracheal trauma: observations on its pathogenesis and its prevention following prolonged orotracheal intubation in the adult. The authors have clinically examined the larynges of 117 patients who were successfully extubated in a Respiratory Care Unit, and have examined the larynges at autopsy from an additional 68 patients who failed to survive in the same Respiratory Care Unit. Based on these observations, our understanding of the pathogenesis of laryngotracheal trauma has allowed us to modify our clinical management in order to minimize such damage. Influence of this study on the management of patients is described."}
{"id": "PMID:459708", "title": "Biliary cholesterol absorption in normal and L-thyroxin-fed rats.", "content": "Infusion of bile containing labeled cholesterol into bile fistula rats has permitted an in vivo study of the movements and of the absorption of biliary cholesterol in the digestive tract. The specific activities of cholesterol were similar in the micelles and the sediment of the luminal content after a 6 hr infusion, indicating rapid exchange of cholesterol between these fractions. In animals fed a basal diet, the biliary cholesterol absorption was higher (83%) than that of dietary cholesterol (70%). Bile cholesterol is essentially absorbed in the jejunum while the absorption of cholesterol from the diet takes place all along the small intestine but preferentially in its second and third quarters. Both alimentary cholesterol and bile cholesterol enter the top cells of the villi in preference to those of the crypts. In L-thyroxin-fed rats, a parallel decrease in biliary and dietary cholesterol absorption was observed. The increase in the intestinal transit of cholesterol and epithelium cell renewal of the jejunum accounted for this observation.", "contents": "Biliary cholesterol absorption in normal and L-thyroxin-fed rats. Infusion of bile containing labeled cholesterol into bile fistula rats has permitted an in vivo study of the movements and of the absorption of biliary cholesterol in the digestive tract. The specific activities of cholesterol were similar in the micelles and the sediment of the luminal content after a 6 hr infusion, indicating rapid exchange of cholesterol between these fractions. In animals fed a basal diet, the biliary cholesterol absorption was higher (83%) than that of dietary cholesterol (70%). Bile cholesterol is essentially absorbed in the jejunum while the absorption of cholesterol from the diet takes place all along the small intestine but preferentially in its second and third quarters. Both alimentary cholesterol and bile cholesterol enter the top cells of the villi in preference to those of the crypts. In L-thyroxin-fed rats, a parallel decrease in biliary and dietary cholesterol absorption was observed. The increase in the intestinal transit of cholesterol and epithelium cell renewal of the jejunum accounted for this observation."}
{"id": "PMID:459709", "title": "Perinatal development of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in rat lung, liver and brain.", "content": "The developmental pattern of microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.34), which catalyzes the rate-limiting step of cholesterol biosynthesis, was studied in lung, liver and brain of Sprague-Dawley rats. Each tissue exhibited a distinct pattern. Reductase activity in the fetal lung reached a peak at 19 days of gestation, which corresponds to the onset of active surfactant production. This observation is consistent with the suggestion that the fetal lung synthesizes all surfactant components including cholesterol. In the liver, reductase activity varied in a reciprocal fashion with serum cholesterol levels. The peak of brain reductase activity occurred at 3 days after birth at the onset of rapid brain growth despite rapidly rising serum cholesterol levels.", "contents": "Perinatal development of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in rat lung, liver and brain. The developmental pattern of microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.34), which catalyzes the rate-limiting step of cholesterol biosynthesis, was studied in lung, liver and brain of Sprague-Dawley rats. Each tissue exhibited a distinct pattern. Reductase activity in the fetal lung reached a peak at 19 days of gestation, which corresponds to the onset of active surfactant production. This observation is consistent with the suggestion that the fetal lung synthesizes all surfactant components including cholesterol. In the liver, reductase activity varied in a reciprocal fashion with serum cholesterol levels. The peak of brain reductase activity occurred at 3 days after birth at the onset of rapid brain growth despite rapidly rising serum cholesterol levels."}
{"id": "PMID:459710", "title": "De novo fatty acid synthesis and fatty acid elongation catalyzed by subcellular fractions from hog and human aorta.", "content": "De novo synthesis and mitochondrial elongation of fatty acids have been demonstrated in subcellular fractions from hog and human aorta. Microsomal fatty acid elongation has been shown in hog aorta. The activity catalyzing the formation of fatty acids from acetyl and malonyl CoA was associated with a high molecular weight complex in the 6 x 10(6) g x min supernatant fraction. The principal product was palmitic acid. Some myristic and stearic acids were also formed. One elongation system was associated with protein which sedimented between 4500 g x min and 150,000 g x min. It used acetyl CoA but not malonyl CoA, and NADH was the preferred reducing agent. Radioactivity from acetyl CoA was incorporated into many fatty acids. In hog aorta a second elongation system was found associated with protein which sedimented at 6 x 10(6) g x min. It used malonyl CoA preferentially as substrate and either NADH or NADPH as reducing agent.", "contents": "De novo fatty acid synthesis and fatty acid elongation catalyzed by subcellular fractions from hog and human aorta. De novo synthesis and mitochondrial elongation of fatty acids have been demonstrated in subcellular fractions from hog and human aorta. Microsomal fatty acid elongation has been shown in hog aorta. The activity catalyzing the formation of fatty acids from acetyl and malonyl CoA was associated with a high molecular weight complex in the 6 x 10(6) g x min supernatant fraction. The principal product was palmitic acid. Some myristic and stearic acids were also formed. One elongation system was associated with protein which sedimented between 4500 g x min and 150,000 g x min. It used acetyl CoA but not malonyl CoA, and NADH was the preferred reducing agent. Radioactivity from acetyl CoA was incorporated into many fatty acids. In hog aorta a second elongation system was found associated with protein which sedimented at 6 x 10(6) g x min. It used malonyl CoA preferentially as substrate and either NADH or NADPH as reducing agent."}
{"id": "PMID:459711", "title": "Age-related changes in glycerolipid formation in lean and obese Zucker rats.", "content": "Age-related changes in hepatic and adipose glycerolipid formation have been described in Zucker rats. Glycerolipid formation was measured in vitro in the presence of [14C]glycerol-3-phosphate, palmitate, ATP, CoA, and Mg2+ by using liver and adipose tissue homogenates derived from various age groups of animals. Hepatic glycerolipid formation increased after birth to reach a peak value at 1 day of age. This period was followed by a decline in the rates of glycerolipid formation. Hepatic glycerolipid formation increased again at the time of weaning and continued to rise up to 32 days in lean rats and 42-44 days in obese rats. Obesity in rats was recognizable at the age of 32 days and was associated with increased rates of glycerolipid formation in both liver and adipose tissue. As far as the changes in hepatic glycerolipid formation and triglyceride accumulation are concerned, obese rats showed more resemblance to 1-day-old rats than to lean animals of similar age groups. Glycerolipid formation decreased in liver and increased in adipose tissue with age in both lean and obese rats. These studies suggest that hepatic and adipose tissue glycerolipid formation is significantly influenced by age and obesity in Zucker rats.", "contents": "Age-related changes in glycerolipid formation in lean and obese Zucker rats. Age-related changes in hepatic and adipose glycerolipid formation have been described in Zucker rats. Glycerolipid formation was measured in vitro in the presence of [14C]glycerol-3-phosphate, palmitate, ATP, CoA, and Mg2+ by using liver and adipose tissue homogenates derived from various age groups of animals. Hepatic glycerolipid formation increased after birth to reach a peak value at 1 day of age. This period was followed by a decline in the rates of glycerolipid formation. Hepatic glycerolipid formation increased again at the time of weaning and continued to rise up to 32 days in lean rats and 42-44 days in obese rats. Obesity in rats was recognizable at the age of 32 days and was associated with increased rates of glycerolipid formation in both liver and adipose tissue. As far as the changes in hepatic glycerolipid formation and triglyceride accumulation are concerned, obese rats showed more resemblance to 1-day-old rats than to lean animals of similar age groups. Glycerolipid formation decreased in liver and increased in adipose tissue with age in both lean and obese rats. These studies suggest that hepatic and adipose tissue glycerolipid formation is significantly influenced by age and obesity in Zucker rats."}
{"id": "PMID:459712", "title": "Effects of diets high in saturated fat and cholesterol on the lipid composition of canine platelets.", "content": "The phospholipid composition of platelets from dogs on various experimental diets was determined. Thyroidectomized foxhounds were fed a control diet or the control diet supplemented with (1) beef tallow, (2) beef tallow and cholesterol, or (3) beef tallow, cholesterol, and safflower oil for 23 weeks prior to isolation of platelets. Platelets from animals fed the control diet contained 36.7% phosphatidylcholine (PC), 22.8% phosphatidylethanolamine (PE), 18.4% sphingomyelin (Sph), 11.8% phosphatidylserine (PS), 6.3% phosphatidylinositol (PI), and 2.2% lysophosphatidylcholine. The PE was 77.6% in the plasmalogen form. No highly significant changes in the phospholipid class composition resulted from the experimental diets. Cholesterol supplementation of the diets, however, caused consistent alterations in the fatty acid compositions of the platelet phospholipids including increases in the percentages of 18:1 omega 9 (oleic acid), 18:2 omega 6 (linoleic acid), and 20:3 omega 6 (homo-gamma linolenic acid) and a decrease in the percentage of 20:4 omega 6 (arachidonic acid). Addition of safflower oil to the tallow-cholesterol diet partially reversed these effects. These cholesterol-induced alterations in fatty acid composition could be due to exchange with plasma lipids, de novo synthesis, or altered platelet metabolism. The mechanism remains to be determined.", "contents": "Effects of diets high in saturated fat and cholesterol on the lipid composition of canine platelets. The phospholipid composition of platelets from dogs on various experimental diets was determined. Thyroidectomized foxhounds were fed a control diet or the control diet supplemented with (1) beef tallow, (2) beef tallow and cholesterol, or (3) beef tallow, cholesterol, and safflower oil for 23 weeks prior to isolation of platelets. Platelets from animals fed the control diet contained 36.7% phosphatidylcholine (PC), 22.8% phosphatidylethanolamine (PE), 18.4% sphingomyelin (Sph), 11.8% phosphatidylserine (PS), 6.3% phosphatidylinositol (PI), and 2.2% lysophosphatidylcholine. The PE was 77.6% in the plasmalogen form. No highly significant changes in the phospholipid class composition resulted from the experimental diets. Cholesterol supplementation of the diets, however, caused consistent alterations in the fatty acid compositions of the platelet phospholipids including increases in the percentages of 18:1 omega 9 (oleic acid), 18:2 omega 6 (linoleic acid), and 20:3 omega 6 (homo-gamma linolenic acid) and a decrease in the percentage of 20:4 omega 6 (arachidonic acid). Addition of safflower oil to the tallow-cholesterol diet partially reversed these effects. These cholesterol-induced alterations in fatty acid composition could be due to exchange with plasma lipids, de novo synthesis, or altered platelet metabolism. The mechanism remains to be determined."}
{"id": "PMID:459713", "title": "Utilization of various sterols by lecithin-cholesterol acyltransferase as acyl acceptors.", "content": "Highly purified lecithin-cholesterol acyltransferase of human plasma was used to study the utilization of various sterols as the acyl acceptor. The esterification of sterols was facilitated by the presence of a 3beta-hydroxyl group and the trans configuration of the A/B rings, as was evident from the lack of acceptor activity of all 3 alpha-hydroxy sterols tested and coprostanol. Cholesterol analogs in which the side chain is modified, such as campesterol, beta-sitosterol, desmosterol and stigmasterol, were less effective than cholesterol as acyl acceptors. However, androstan-3 beta-ol, which completely lacks the side chain, was found to be more active than cholesterol. The transfer of the acyl group to all effective sterols required the presence of the cofactor peptide apolipoprotein A-I.", "contents": "Utilization of various sterols by lecithin-cholesterol acyltransferase as acyl acceptors. Highly purified lecithin-cholesterol acyltransferase of human plasma was used to study the utilization of various sterols as the acyl acceptor. The esterification of sterols was facilitated by the presence of a 3beta-hydroxyl group and the trans configuration of the A/B rings, as was evident from the lack of acceptor activity of all 3 alpha-hydroxy sterols tested and coprostanol. Cholesterol analogs in which the side chain is modified, such as campesterol, beta-sitosterol, desmosterol and stigmasterol, were less effective than cholesterol as acyl acceptors. However, androstan-3 beta-ol, which completely lacks the side chain, was found to be more active than cholesterol. The transfer of the acyl group to all effective sterols required the presence of the cofactor peptide apolipoprotein A-I."}
{"id": "PMID:459714", "title": "Simultaneous mass spectrometric measurement of prostaglandins E1 (PGE1) and PGE2 with a deuterated internal standard.", "content": "The use of (3,3,4,4-2H4)PGE2 as internal standard for the quantitative measurement of PGE1 by gas chromatography-mass spectrometry is reported. A method for the simultaneous analysis of PGE1 and PGE2 is also described. The prostaglandins were analyzed by selected ion monitoring as the methyl ester-trimethylsilyl ether (ME-TMS) derivatives of PGB1 and PGB2, respectively. In all cases, a linear response over the range 1 to 70 ng (2.8 to 198 pmoles) was demonstrated. The use of this procedure is exemplified by its application to the PG analysis of sheep seminal vesicles.", "contents": "Simultaneous mass spectrometric measurement of prostaglandins E1 (PGE1) and PGE2 with a deuterated internal standard. The use of (3,3,4,4-2H4)PGE2 as internal standard for the quantitative measurement of PGE1 by gas chromatography-mass spectrometry is reported. A method for the simultaneous analysis of PGE1 and PGE2 is also described. The prostaglandins were analyzed by selected ion monitoring as the methyl ester-trimethylsilyl ether (ME-TMS) derivatives of PGB1 and PGB2, respectively. In all cases, a linear response over the range 1 to 70 ng (2.8 to 198 pmoles) was demonstrated. The use of this procedure is exemplified by its application to the PG analysis of sheep seminal vesicles."}
{"id": "PMID:459715", "title": "Desaturation of eicosa-11,14-dienoic acid in human testes.", "content": "The metabolism of [1-14C]eicosa-11,14-dienoic acid was investigated in human testes using whole tissue minces and microsomal preparations. Both types of preparations catalyzed the desaturation of the labeled diene to eicosa-8,11,14-trienoic as well as eicosa-5,11,14-trienoic acid. The reported results, therefore, indicate that human testicular tissue, as well as rat testicular tissue (reported previously), is capable of utilizing eicosa-11,14-dienoic acid as a precursor of arachidonic acid. Since it is known that there is no delta 8 desaturase activity in rat liver and brain, these studies support the concept that there is a tissue variation in this enzymatic pathway.", "contents": "Desaturation of eicosa-11,14-dienoic acid in human testes. The metabolism of [1-14C]eicosa-11,14-dienoic acid was investigated in human testes using whole tissue minces and microsomal preparations. Both types of preparations catalyzed the desaturation of the labeled diene to eicosa-8,11,14-trienoic as well as eicosa-5,11,14-trienoic acid. The reported results, therefore, indicate that human testicular tissue, as well as rat testicular tissue (reported previously), is capable of utilizing eicosa-11,14-dienoic acid as a precursor of arachidonic acid. Since it is known that there is no delta 8 desaturase activity in rat liver and brain, these studies support the concept that there is a tissue variation in this enzymatic pathway."}
{"id": "PMID:459716", "title": "beta-Oxidation of the coenzyme A esters of vaccenic, elaidic, and petroselaidic acids by rat heart mitochondria.", "content": "Rates of beta-oxidation of the coenzyme A esters of vaccenic, elaidic, and petroselaidic acids as well as their respective cis isomers by rat heart mitochondria were measured and compared. At all concentrations studied, vaccenoyl-CoA was oxidized more rapidly than elaidoyl-CoA, but more slowly than oleoyl-CoA except at high substrate concentrations. All trans octadecenoyl-CoA esters were oxidized at a slower rate than their respective cis or saturated isomers. Oxidation rates decreased as the double bond approached the carboxyl-end.", "contents": "beta-Oxidation of the coenzyme A esters of vaccenic, elaidic, and petroselaidic acids by rat heart mitochondria. Rates of beta-oxidation of the coenzyme A esters of vaccenic, elaidic, and petroselaidic acids as well as their respective cis isomers by rat heart mitochondria were measured and compared. At all concentrations studied, vaccenoyl-CoA was oxidized more rapidly than elaidoyl-CoA, but more slowly than oleoyl-CoA except at high substrate concentrations. All trans octadecenoyl-CoA esters were oxidized at a slower rate than their respective cis or saturated isomers. Oxidation rates decreased as the double bond approached the carboxyl-end."}
{"id": "PMID:459717", "title": "The formation of phosphatidylinositol by acylation of 2-acyl-sn-glycero-3-phosphorylinositol in rat liver microsomes.", "content": "The conversion of 2-acyl-sn-glycero-3-phosphorylinositol into phosphatidylinositol via acyl-CoA: 2-acyl-sn-glycero-3-phosphorylinositol acyltransferase activity was found to occur in rat liver microsomes. Over a wide range of conditions, stearic acid was preferred over palmitate by the acyltransferase when these acids were presented in mixtures as acyl-CoA derivatives. The potential importance of this enzyme activity for the entry of stearic acid into the 1-position of hepatic phosphatidylinositol is further supported by its greater preference for stearate relative to the acyl-CoA:2-acyl-sn-glycero-3-phosphorylcholine acyltransferase under certain assay conditions.", "contents": "The formation of phosphatidylinositol by acylation of 2-acyl-sn-glycero-3-phosphorylinositol in rat liver microsomes. The conversion of 2-acyl-sn-glycero-3-phosphorylinositol into phosphatidylinositol via acyl-CoA: 2-acyl-sn-glycero-3-phosphorylinositol acyltransferase activity was found to occur in rat liver microsomes. Over a wide range of conditions, stearic acid was preferred over palmitate by the acyltransferase when these acids were presented in mixtures as acyl-CoA derivatives. The potential importance of this enzyme activity for the entry of stearic acid into the 1-position of hepatic phosphatidylinositol is further supported by its greater preference for stearate relative to the acyl-CoA:2-acyl-sn-glycero-3-phosphorylcholine acyltransferase under certain assay conditions."}
{"id": "PMID:459718", "title": "The influence of phthalate esters on human plasma lecithin/cholesterol acyltransferase.", "content": "The effect of various phthalate esters on the lecithin/cholesterol acyltransferase activity in man was studied in vitro. The enzymatic activity was strongly reduced with all phthalates except for the dimethyl phthalate. The inhibition rate depends on the phthalate concentration and also on the carbon number of the alkyl groups of phthalates.", "contents": "The influence of phthalate esters on human plasma lecithin/cholesterol acyltransferase. The effect of various phthalate esters on the lecithin/cholesterol acyltransferase activity in man was studied in vitro. The enzymatic activity was strongly reduced with all phthalates except for the dimethyl phthalate. The inhibition rate depends on the phthalate concentration and also on the carbon number of the alkyl groups of phthalates."}
{"id": "PMID:459719", "title": "Isoflavones and hypercholesterolemia in rats.", "content": "Isoflavones isolated from three commonly used pulses such as Bengalgram (Cicer arietinum), greengram (Phaseolus aureus) and blackgram (Phaseolus mungo) and p-coumaric acid were supplemented to hypercholesterolemia-inducing diet of rats. Among isoflavones, Biochanin A and Formononetin showed hypolipidemic activity but diadzein did not; p-coumaric acid also produced a significant reduction in serum cholesterol levels.", "contents": "Isoflavones and hypercholesterolemia in rats. Isoflavones isolated from three commonly used pulses such as Bengalgram (Cicer arietinum), greengram (Phaseolus aureus) and blackgram (Phaseolus mungo) and p-coumaric acid were supplemented to hypercholesterolemia-inducing diet of rats. Among isoflavones, Biochanin A and Formononetin showed hypolipidemic activity but diadzein did not; p-coumaric acid also produced a significant reduction in serum cholesterol levels."}
{"id": "PMID:459720", "title": "Variations in the molecular species of lung phosphatidylglycerol.", "content": "Approximately 20% of the phosphatidylglycerol of the lung tissue of several animals was found to have both fatty acids saturated. Pulmonary washings from the lung of the rabbit and guinea pig had more saturated phosphatidylglycerol than the washed lung tissue. Lung-saturated phosphatidylglycerol was relatively low in the perinatal period, a time during which saturated phosphatidylcholine accumulated predominantly. This suggests that the metabolism of the saturated species of lung phosphatidylcholine and phosphatidylglycerol, which are considered to be the major pulmonary surfactants, may not be regulated in the same manner, at least in the perinatal lung.", "contents": "Variations in the molecular species of lung phosphatidylglycerol. Approximately 20% of the phosphatidylglycerol of the lung tissue of several animals was found to have both fatty acids saturated. Pulmonary washings from the lung of the rabbit and guinea pig had more saturated phosphatidylglycerol than the washed lung tissue. Lung-saturated phosphatidylglycerol was relatively low in the perinatal period, a time during which saturated phosphatidylcholine accumulated predominantly. This suggests that the metabolism of the saturated species of lung phosphatidylcholine and phosphatidylglycerol, which are considered to be the major pulmonary surfactants, may not be regulated in the same manner, at least in the perinatal lung."}
{"id": "PMID:459721", "title": "Incorporation of deuterium-labeled cis- and trans-9-octadecenoic acids in humans: plasma, erythrocyte, and platelet phospholipids.", "content": "The objective of this study was to follow the uptake and distribution of oleic and elaidic acids into human erythrocytes, platelets, and plasma phospholipids. The use of dual and triple labeling methodology permitted a precise comparison of elaidic and oleic acid utilization. Elaidic acid (EI) was selectively concentrated in all the plasma phospholipids except for lysophosphatidylcholine. Three times more elaidic than oleic acid (OI) accumulated in the 1-acyl position of phosphatidylcholine, as determined by hydrolysis with phospholipase A2. Rapid incorporation and removal of elaidate were observed for all samples. These results support the concept that enzymes responsible for acylation of phospholipids are sensitive to double bond configuration and the physical properties of the fatty acid moieties. Labeled fatty acid levels in red cell and platelet phospholipids were much lower than for plasma phospholipids, indicating a relatively slow rate for the in vivo incorporation of fatty acids into blood cell membrane phospholipids. No isotope effect was found when oleic acid labeled with deuterium on the double bond was used.", "contents": "Incorporation of deuterium-labeled cis- and trans-9-octadecenoic acids in humans: plasma, erythrocyte, and platelet phospholipids. The objective of this study was to follow the uptake and distribution of oleic and elaidic acids into human erythrocytes, platelets, and plasma phospholipids. The use of dual and triple labeling methodology permitted a precise comparison of elaidic and oleic acid utilization. Elaidic acid (EI) was selectively concentrated in all the plasma phospholipids except for lysophosphatidylcholine. Three times more elaidic than oleic acid (OI) accumulated in the 1-acyl position of phosphatidylcholine, as determined by hydrolysis with phospholipase A2. Rapid incorporation and removal of elaidate were observed for all samples. These results support the concept that enzymes responsible for acylation of phospholipids are sensitive to double bond configuration and the physical properties of the fatty acid moieties. Labeled fatty acid levels in red cell and platelet phospholipids were much lower than for plasma phospholipids, indicating a relatively slow rate for the in vivo incorporation of fatty acids into blood cell membrane phospholipids. No isotope effect was found when oleic acid labeled with deuterium on the double bond was used."}
{"id": "PMID:459722", "title": "Uptake of [14C]choline and incorporation into lung phospholipid by the isolated perfused rat lung.", "content": "We have used the isolated perfused lung (IPL) preparation from the rat to determine whether uptake of choline from the vascular compartment could limit the rate of synthesis of phosphatidyl-choline (PC). The uptake of choline was rapid and did not saturate at a concentration of 10 mM. The rate of incorporation of choline into phospholipid was saturated above 0.1 mM choline. Whereas, uptake and incorporation were depressed at 4 C, uptake was neither dependent on the extracellular sodium concentration nor inhibited by equimolar concentrations of hemicholinium-3 (HC-3). We could find no evidence that uptake might limit synthesis of lung lecithin and conclude that uptake is either by free diffusion, or by a carrier-mediated process with a very high Km.", "contents": "Uptake of [14C]choline and incorporation into lung phospholipid by the isolated perfused rat lung. We have used the isolated perfused lung (IPL) preparation from the rat to determine whether uptake of choline from the vascular compartment could limit the rate of synthesis of phosphatidyl-choline (PC). The uptake of choline was rapid and did not saturate at a concentration of 10 mM. The rate of incorporation of choline into phospholipid was saturated above 0.1 mM choline. Whereas, uptake and incorporation were depressed at 4 C, uptake was neither dependent on the extracellular sodium concentration nor inhibited by equimolar concentrations of hemicholinium-3 (HC-3). We could find no evidence that uptake might limit synthesis of lung lecithin and conclude that uptake is either by free diffusion, or by a carrier-mediated process with a very high Km."}
{"id": "PMID:459723", "title": "A simplified procedure for the quantitative extraction of lipids from brain tissue.", "content": "A method is described for the quantitative extraction of lipid from brain tissue with chloroform/methanol (C/M) that eliminates secondary purification of the lipid extract by dextran-gel chromatography or aqueous washing of the organic extract. Nonlipid substances that generally contaminate C/M lipid extracts are separated by pre-extraction of the tissue with dilute (0.25%) aqueous acetic acid. The residual tissue is extracted twice with 40 volumes of C/M (1:1, v/v). Approximately 97% of the lipid is recovered in these extractions. A third extraction which yields ca. 1% more lipid is performed if the process is discontinued at this stage in a shortened version of the method. The remainder of the lipid is recovered after treatment of the tissue with 1 N HC1 by two additional extractions, the first with 40 volumes of C/M (1:2, v/v) and the second with 40 volumes of methanol. The method, which was demonstrated with pig brain, gave a complete extraction of the lipid, including gangliosides, free of nonlipid substances.", "contents": "A simplified procedure for the quantitative extraction of lipids from brain tissue. A method is described for the quantitative extraction of lipid from brain tissue with chloroform/methanol (C/M) that eliminates secondary purification of the lipid extract by dextran-gel chromatography or aqueous washing of the organic extract. Nonlipid substances that generally contaminate C/M lipid extracts are separated by pre-extraction of the tissue with dilute (0.25%) aqueous acetic acid. The residual tissue is extracted twice with 40 volumes of C/M (1:1, v/v). Approximately 97% of the lipid is recovered in these extractions. A third extraction which yields ca. 1% more lipid is performed if the process is discontinued at this stage in a shortened version of the method. The remainder of the lipid is recovered after treatment of the tissue with 1 N HC1 by two additional extractions, the first with 40 volumes of C/M (1:2, v/v) and the second with 40 volumes of methanol. The method, which was demonstrated with pig brain, gave a complete extraction of the lipid, including gangliosides, free of nonlipid substances."}
{"id": "PMID:459772", "title": "[Thermostat electronic circuit for vital microscopy].", "content": "To provide optimal temperature conditions needed for vital microscopy, an electronic circuit for the thermostat has been elaborated. This unit is designed to heat and maintain at a definite temperature level the table for manipulations and the microscope objective. Thermoresistor MMT-4 served as a temperature transducer.", "contents": "[Thermostat electronic circuit for vital microscopy]. To provide optimal temperature conditions needed for vital microscopy, an electronic circuit for the thermostat has been elaborated. This unit is designed to heat and maintain at a definite temperature level the table for manipulations and the microscope objective. Thermoresistor MMT-4 served as a temperature transducer."}
{"id": "PMID:459771", "title": "[Evaluation of linear distortions in rheography].", "content": "Methods permitting to estimate parameters of hemodynamics through bioimpedance measurements have gained ground in study and diagnosis of the cardiovascular system. The error in bioimpedance measurement depends considerably on dynamic characteristics of the measuring devices being employed. The dynamic characteristics can be investigated by the method evaluating linear distortions. A relation can be established between these characteristics and errors of measuring major informative parameters of a rheographic signal.", "contents": "[Evaluation of linear distortions in rheography]. Methods permitting to estimate parameters of hemodynamics through bioimpedance measurements have gained ground in study and diagnosis of the cardiovascular system. The error in bioimpedance measurement depends considerably on dynamic characteristics of the measuring devices being employed. The dynamic characteristics can be investigated by the method evaluating linear distortions. A relation can be established between these characteristics and errors of measuring major informative parameters of a rheographic signal."}
{"id": "PMID:459773", "title": "[Reading device for the blind based on the transformation of the optical image into a vibrotactile one].", "content": "The article describes a device in which the printed text letters after their photo-transforming are presented as a matrix of bimorphous piezoelements in the vibrotactile form. The operation of a bimorph as an element of a tactile matrix has been studied experimentally and requirements to parameters of excitation signals were settled. Consideration is given to the phototactile transformation principle and to some circuits for its practical embodiment.", "contents": "[Reading device for the blind based on the transformation of the optical image into a vibrotactile one]. The article describes a device in which the printed text letters after their photo-transforming are presented as a matrix of bimorphous piezoelements in the vibrotactile form. The operation of a bimorph as an element of a tactile matrix has been studied experimentally and requirements to parameters of excitation signals were settled. Consideration is given to the phototactile transformation principle and to some circuits for its practical embodiment."}
{"id": "PMID:459774", "title": "[Effect of the filler and liquid medium on the surface quality in the vibration treatment of medical equipment products].", "content": "The surface quality of products made from stainless steel 40--13 are determined mainly by methods and parameters used for their final processing. Investigation of technological characteristics of different fillers used in vibration processing and, as well, the influence of polyvinyl emulsion-water solution has shown that T-trihedral granules (made at the Moscow Plant of Abrasive Articles) can be the choice for the vibration processing of details of stainless steel 40 X 13. The polymeric component in the liquid technological medium, instead of Na2CO3 water-solution, permits a threefold increase in the metal removal, improves the surface roughness by 1.5 times, and considerably improves the surface reflectivity.", "contents": "[Effect of the filler and liquid medium on the surface quality in the vibration treatment of medical equipment products]. The surface quality of products made from stainless steel 40--13 are determined mainly by methods and parameters used for their final processing. Investigation of technological characteristics of different fillers used in vibration processing and, as well, the influence of polyvinyl emulsion-water solution has shown that T-trihedral granules (made at the Moscow Plant of Abrasive Articles) can be the choice for the vibration processing of details of stainless steel 40 X 13. The polymeric component in the liquid technological medium, instead of Na2CO3 water-solution, permits a threefold increase in the metal removal, improves the surface roughness by 1.5 times, and considerably improves the surface reflectivity."}
{"id": "PMID:459776", "title": "[Methodological bases for designing multipurpose laboratories].", "content": "A well-grounded selection of the necessary minimum of measuring facilities is of paramount importance in projecting multipurpose laboratories. These facilities should ensure the required accuracy of analyses to be carried out, and measurements covering the whole dynamic range of the values variations. It was suggested to organise new multipurpose laboratories in accordance with technical and economical reasoning and with the calculated generalized index which shows a compliance of technical characteristics of the measuring equipment chosen with the methodical demands.", "contents": "[Methodological bases for designing multipurpose laboratories]. A well-grounded selection of the necessary minimum of measuring facilities is of paramount importance in projecting multipurpose laboratories. These facilities should ensure the required accuracy of analyses to be carried out, and measurements covering the whole dynamic range of the values variations. It was suggested to organise new multipurpose laboratories in accordance with technical and economical reasoning and with the calculated generalized index which shows a compliance of technical characteristics of the measuring equipment chosen with the methodical demands."}
{"id": "PMID:459777", "title": "[Definition of the nomenclature for quality indices for medical equipment products].", "content": "The qualimetric approach to the definition of qualitative indices for the medical engineering products allows to propose five initial levels of the \"index-tree\" with the use of the indices nomenclature comprised in GOST--22.851-77. Due to the proposed method of the expert opinions' formal processing, classification structure of qualitative properties for these products has been revealed.", "contents": "[Definition of the nomenclature for quality indices for medical equipment products]. The qualimetric approach to the definition of qualitative indices for the medical engineering products allows to propose five initial levels of the \"index-tree\" with the use of the indices nomenclature comprised in GOST--22.851-77. Due to the proposed method of the expert opinions' formal processing, classification structure of qualitative properties for these products has been revealed."}
{"id": "PMID:459778", "title": "[Improvement in the methods of performing the work in assuring the operating characteristics of medical equipment].", "content": "Improvement of operation methods that ensure reliability, climatic and mechanical stability, strength and other service qualities of medical equipment is now accomplished under conditions of intimate cooperation between constructors and manufactures, introduction of progressive norms and approaches for the provision and verification of the equipment operation qualities, building and employment of common test facilities.", "contents": "[Improvement in the methods of performing the work in assuring the operating characteristics of medical equipment]. Improvement of operation methods that ensure reliability, climatic and mechanical stability, strength and other service qualities of medical equipment is now accomplished under conditions of intimate cooperation between constructors and manufactures, introduction of progressive norms and approaches for the provision and verification of the equipment operation qualities, building and employment of common test facilities."}
{"id": "PMID:459782", "title": "[Blood heat exchangers for the artificial heart using a radioisotope power source].", "content": "A design of a vascular heat exchanger enabling it to establish in experiments with animals the degree of the possible specific-capacity thermal loads has been devised. The performance results may be taken account of in designing implantable appliances of assistive circulation and artificial heart.", "contents": "[Blood heat exchangers for the artificial heart using a radioisotope power source]. A design of a vascular heat exchanger enabling it to establish in experiments with animals the degree of the possible specific-capacity thermal loads has been devised. The performance results may be taken account of in designing implantable appliances of assistive circulation and artificial heart."}
{"id": "PMID:459780", "title": "[New model of laboratory contact microscope].", "content": "A new model of a laboratory contact microscope is described that keeps the ocular and the photocamera motionless during their focusing. The latter is performed by means of a moving positive lens placed between the objective and the opaque illuminator. The new focusing approach allows different attachements to be used, such as photometric, binocular etc. The microscope enables one to reach the stereoscopic effect that can be intensified diaphragms located at exit pupils. The opaque illuminator of a new type incorporated into the microscope gives a considerable gain in light brightness.", "contents": "[New model of laboratory contact microscope]. A new model of a laboratory contact microscope is described that keeps the ocular and the photocamera motionless during their focusing. The latter is performed by means of a moving positive lens placed between the objective and the opaque illuminator. The new focusing approach allows different attachements to be used, such as photometric, binocular etc. The microscope enables one to reach the stereoscopic effect that can be intensified diaphragms located at exit pupils. The opaque illuminator of a new type incorporated into the microscope gives a considerable gain in light brightness."}
{"id": "PMID:459783", "title": "[Loco-regional anesthesia in remote medical units. I. - Basis (author's transl)].", "content": "This paper is a foreword to a series of articles about anesthesia in small medical units in tropical areas. When general anesthesia must be discarded in regard to the patient's condition or the poor level of facilities, loco-regional anesthesia proves to be of great value. The authors review the main drugs available, their dosages, accidents, counter-indications and pre-anesthetic cares.", "contents": "[Loco-regional anesthesia in remote medical units. I. - Basis (author's transl)]. This paper is a foreword to a series of articles about anesthesia in small medical units in tropical areas. When general anesthesia must be discarded in regard to the patient's condition or the poor level of facilities, loco-regional anesthesia proves to be of great value. The authors review the main drugs available, their dosages, accidents, counter-indications and pre-anesthetic cares."}
{"id": "PMID:459784", "title": "[Histo-pathological features of African mycetomas (author's transl)].", "content": "From a review of 94 original cases of mycetoma, a description of the most important histo-pathological features of these tumors is given. The microscopic diagnosis of mycetoma is easy and so is generally the identification of the responsible agent, either a bacteria or a fungus. In every case and specially in atypic ones a mycological study is necessary to ascertain the aetiological diagnosis.", "contents": "[Histo-pathological features of African mycetomas (author's transl)]. From a review of 94 original cases of mycetoma, a description of the most important histo-pathological features of these tumors is given. The microscopic diagnosis of mycetoma is easy and so is generally the identification of the responsible agent, either a bacteria or a fungus. In every case and specially in atypic ones a mycological study is necessary to ascertain the aetiological diagnosis."}
{"id": "PMID:459785", "title": "[Value of radiology for the diagnosis of mycetomas (author's transl)].", "content": "Mycetomas have raised a new interest because of cases introduced from tropical areas to non endemic countries, and because of the emergence in these countries of autochthonous cases in immuno-depressed patients. Radiologic diagnosis is no more limited to the description of osseous changes, the basic aspects of which are characteristic: cortical erosion, periosteous condensation and reaction. Using modern radiological technics such as arteriography and lymphography, gives a possibility to appreciate more thoroughly the extension and the potential evolution, so that the radiological approach may be similar to that of carcinology.", "contents": "[Value of radiology for the diagnosis of mycetomas (author's transl)]. Mycetomas have raised a new interest because of cases introduced from tropical areas to non endemic countries, and because of the emergence in these countries of autochthonous cases in immuno-depressed patients. Radiologic diagnosis is no more limited to the description of osseous changes, the basic aspects of which are characteristic: cortical erosion, periosteous condensation and reaction. Using modern radiological technics such as arteriography and lymphography, gives a possibility to appreciate more thoroughly the extension and the potential evolution, so that the radiological approach may be similar to that of carcinology."}
{"id": "PMID:459786", "title": "[Tactic for treatment of African mycetomas (author's transl)].", "content": "The authors report their own experience from 200 cases and they consider the matter from the point of a surgeon operating in a small medical unit in Africa. The clinical and biological aspects are described; the importance and the difficulties of appreciating the real spreading of infection are emphasized. Conventional radiology is of major value. The biological diagnosis may give way to a medical treatment with DDS and sulfamethoxypyridazine in cases due to actinomycetes with red or yellow granules; but in fungic cases fungicid drugs proved to be inefficient. Surgery is still an important component of the treatment. Its tactics are discussed according to the germ, the localization, the extension and also to the social and psychological status, but it is hoped that more sanitary education will reduce its preponderence in making possible earlier diagnosis and treatment.", "contents": "[Tactic for treatment of African mycetomas (author's transl)]. The authors report their own experience from 200 cases and they consider the matter from the point of a surgeon operating in a small medical unit in Africa. The clinical and biological aspects are described; the importance and the difficulties of appreciating the real spreading of infection are emphasized. Conventional radiology is of major value. The biological diagnosis may give way to a medical treatment with DDS and sulfamethoxypyridazine in cases due to actinomycetes with red or yellow granules; but in fungic cases fungicid drugs proved to be inefficient. Surgery is still an important component of the treatment. Its tactics are discussed according to the germ, the localization, the extension and also to the social and psychological status, but it is hoped that more sanitary education will reduce its preponderence in making possible earlier diagnosis and treatment."}
{"id": "PMID:459788", "title": "[A controlled experience with a thermostabile measles vaccine in Cameroon (author's transl)].", "content": "Rimevax, a thermostabile measles vaccine, has been stored, before reconstitution, during seven days at temperature from 23 degrees C to 25 degrees C. This vaccine has given seroconversion in 87 per 100 of the babies vaccinated and aged from 9 to 24 months. This percentage assesses the value of Rimevax for field campaigns.", "contents": "[A controlled experience with a thermostabile measles vaccine in Cameroon (author's transl)]. Rimevax, a thermostabile measles vaccine, has been stored, before reconstitution, during seven days at temperature from 23 degrees C to 25 degrees C. This vaccine has given seroconversion in 87 per 100 of the babies vaccinated and aged from 9 to 24 months. This percentage assesses the value of Rimevax for field campaigns."}
{"id": "PMID:459787", "title": "[Some new cases of congenital human African trypanosomiasis (T. gambiense) (author's transl)].", "content": "The authors report three cases of congenital transmission of T. gambiense. In two cases, the parasite has been found in the blood of the umbilical cord. In the third case, it has been found twenty hours after birth in the peripherical blood of a new born child whose mother was affected by trypanosomiasis. They report also two cases of pregnant mothers with sleeping sickness, whose children were born-unaffected by the disease. The authors believe that the placental transmission of the human african trypanosomiasis is more frequent than thought.", "contents": "[Some new cases of congenital human African trypanosomiasis (T. gambiense) (author's transl)]. The authors report three cases of congenital transmission of T. gambiense. In two cases, the parasite has been found in the blood of the umbilical cord. In the third case, it has been found twenty hours after birth in the peripherical blood of a new born child whose mother was affected by trypanosomiasis. They report also two cases of pregnant mothers with sleeping sickness, whose children were born-unaffected by the disease. The authors believe that the placental transmission of the human african trypanosomiasis is more frequent than thought."}
{"id": "PMID:459790", "title": "[Agents of mycetomas: a bacteriological and parasitological study (author's transl)].", "content": "Microscopic agents responsible of Mycetomas are reviewed. They may be bacteria belonging to the Actinomycetalae order (Nocardia, Actinomadura, Streptomyces) or very various fungi belonging to the classes of Adelomycetes or Ascomycetes (Madurella, Leptosphaeria, Neotestudina, etc.). Though the procedures for collecting and transporting samples are the same, direct microscopic examination gives possibility to choose among the selective culture media the most appropriate ones to isolate either fungi or Actinomycetalae. Technics of identification for the most important bacterial agents are given, but fungi can be identified in only very specialized laboratories. Immunological methods may bring valuable indications for helping diagnosis or treatment supervision.", "contents": "[Agents of mycetomas: a bacteriological and parasitological study (author's transl)]. Microscopic agents responsible of Mycetomas are reviewed. They may be bacteria belonging to the Actinomycetalae order (Nocardia, Actinomadura, Streptomyces) or very various fungi belonging to the classes of Adelomycetes or Ascomycetes (Madurella, Leptosphaeria, Neotestudina, etc.). Though the procedures for collecting and transporting samples are the same, direct microscopic examination gives possibility to choose among the selective culture media the most appropriate ones to isolate either fungi or Actinomycetalae. Technics of identification for the most important bacterial agents are given, but fungi can be identified in only very specialized laboratories. Immunological methods may bring valuable indications for helping diagnosis or treatment supervision."}
{"id": "PMID:459789", "title": "[Paralysis of the opposition of the thumb in leprosy: value of the transplantation of the extensor indicis (author's transl)].", "content": "The transplantation of the extensor indicis to restore the opposition of thumb has been realized by two techniques: -- transplantation through the 2nd interosseous space with suture on the abductor pollicis brevis but the result was poor for the guide pulley resulting did not work well. -- transplantation with reflexion on the cubital side of the wrist which in 5 cases proved to be satisfactory. In medico cubital paralysis one must also cure the \"cubital claw of the thumb\". The surgical procedure, using a dispensable tendon, could be directly associated to the sutures of the proximal injuries of the cubital nerve as a temporary palliative.", "contents": "[Paralysis of the opposition of the thumb in leprosy: value of the transplantation of the extensor indicis (author's transl)]. The transplantation of the extensor indicis to restore the opposition of thumb has been realized by two techniques: -- transplantation through the 2nd interosseous space with suture on the abductor pollicis brevis but the result was poor for the guide pulley resulting did not work well. -- transplantation with reflexion on the cubital side of the wrist which in 5 cases proved to be satisfactory. In medico cubital paralysis one must also cure the \"cubital claw of the thumb\". The surgical procedure, using a dispensable tendon, could be directly associated to the sutures of the proximal injuries of the cubital nerve as a temporary palliative."}
{"id": "PMID:459792", "title": "[Late aspects of palpebral anthrax (author's transl)].", "content": "Report of 5 cases of palpebral anthrax treated after the acute phase, in a cicatricial or precicatricial stade. The authors recommend to delay plastic surgery up to three months and to select total skin graft for upper eye-lid and pediculed skin flap for lower eye-lid.", "contents": "[Late aspects of palpebral anthrax (author's transl)]. Report of 5 cases of palpebral anthrax treated after the acute phase, in a cicatricial or precicatricial stade. The authors recommend to delay plastic surgery up to three months and to select total skin graft for upper eye-lid and pediculed skin flap for lower eye-lid."}
{"id": "PMID:459793", "title": "[Trachoma prevalence in the Sahel: report of a 1978 survey in Gourma area (Mali) (author's transl)].", "content": "Report of a survey conducted in 1978 in the Niger sweep on a population consisting for one third of adults and for two thirds of children and adolescents. 2.571 persons have been controlled, giving evidence of 441 cases of trachoma (17,15 p. 100), 327 of which were evolutive and 114 cicatricial; 30,4 p. 100 of the cases were severe and 10 p. 100 suffered of a loss of visual acuity.", "contents": "[Trachoma prevalence in the Sahel: report of a 1978 survey in Gourma area (Mali) (author's transl)]. Report of a survey conducted in 1978 in the Niger sweep on a population consisting for one third of adults and for two thirds of children and adolescents. 2.571 persons have been controlled, giving evidence of 441 cases of trachoma (17,15 p. 100), 327 of which were evolutive and 114 cicatricial; 30,4 p. 100 of the cases were severe and 10 p. 100 suffered of a loss of visual acuity."}
{"id": "PMID:459949", "title": "Endotoxic glycolipid as a potent depressor of the hepatic drug-metabolizing enzyme systems in mice.", "content": "Inhibitory effects of the endotoxic glycolipid from Salmonella minnesota R595 on hepatic drug-metabolizing enzyme activities in mice were investigated, and the depressor activity of the glycolipid in the enzyme systems was confirmed. Among degradation products of lipopolysaccharides tested, lipid A preparations derived from the mild acetic acid hydrolysates of lipopolysaccharides were the most active, but the lipid A fractions prepared from the hydrolysates with 1 N-HCl were almost inactive. A degraded polysaccharide fraction from E. coli lipopolysaccharide was inactive. The activities of the glycolipid and the lipid A preparation were markedly reduced by treatment with alkaline-hydroxylamine, mild alkali or hydrazine. The data showed that the lipid A moiety of the glycolipid may be responsible for the inhibitory activity on the hepatic drug-metabolizing enzyme systems.", "contents": "Endotoxic glycolipid as a potent depressor of the hepatic drug-metabolizing enzyme systems in mice. Inhibitory effects of the endotoxic glycolipid from Salmonella minnesota R595 on hepatic drug-metabolizing enzyme activities in mice were investigated, and the depressor activity of the glycolipid in the enzyme systems was confirmed. Among degradation products of lipopolysaccharides tested, lipid A preparations derived from the mild acetic acid hydrolysates of lipopolysaccharides were the most active, but the lipid A fractions prepared from the hydrolysates with 1 N-HCl were almost inactive. A degraded polysaccharide fraction from E. coli lipopolysaccharide was inactive. The activities of the glycolipid and the lipid A preparation were markedly reduced by treatment with alkaline-hydroxylamine, mild alkali or hydrazine. The data showed that the lipid A moiety of the glycolipid may be responsible for the inhibitory activity on the hepatic drug-metabolizing enzyme systems."}
{"id": "PMID:459963", "title": "Glycolysis-dependent antimetabolities: possible improvement in antineoplastic selectivity.", "content": "The increased rate of glycolysis in neoplastic cells may be used to design tentatively antimetabolites whose detoxification would be faster in normal cells. Pyruvo-substituted nucleotides are examined in this respect and their expected differential metabolism outlined.", "contents": "Glycolysis-dependent antimetabolities: possible improvement in antineoplastic selectivity. The increased rate of glycolysis in neoplastic cells may be used to design tentatively antimetabolites whose detoxification would be faster in normal cells. Pyruvo-substituted nucleotides are examined in this respect and their expected differential metabolism outlined."}
{"id": "PMID:459964", "title": "The cause of cancer: biochemical defects in the cancer cell demonstrated by the effects of electromagnetic radiation, glucose and oxygen.", "content": "The responses of a person carrying a cancer to 434 MHz electromagnetic radiation are such that they demonstrate that each human cell contains at least two separate respiratory pathways which can convert glucose to energy. The first pathway produces energy by an anaerobic mechanism and it can be demonstrated that this energy is used for the purpose of cell replication. The second pathway(s) produces energy from glucose by aerobic oxidative processes which can be shown to energise other cellular functions. One of these functions is that of controlling its own cell division. Other demonstrable functions of this aerobic energy producing mechanism are concerned with individual cellular repair processes, multicellular organisation and repair and the preservation of gross anatomical perfection. Release of the first or anaerobic system from supervisory control by the second or aerobic sytem(s) permits the unlimited cell division which is the phenomenon known as cancer. The available circumstantial evidence suggests that this is not the result of nuclear or chromosomal defects or mutations but is due to direct irreversible sublethal damage to the cellular aerobic glucose metabolic system whilst the anaerobic system remains intact.", "contents": "The cause of cancer: biochemical defects in the cancer cell demonstrated by the effects of electromagnetic radiation, glucose and oxygen. The responses of a person carrying a cancer to 434 MHz electromagnetic radiation are such that they demonstrate that each human cell contains at least two separate respiratory pathways which can convert glucose to energy. The first pathway produces energy by an anaerobic mechanism and it can be demonstrated that this energy is used for the purpose of cell replication. The second pathway(s) produces energy from glucose by aerobic oxidative processes which can be shown to energise other cellular functions. One of these functions is that of controlling its own cell division. Other demonstrable functions of this aerobic energy producing mechanism are concerned with individual cellular repair processes, multicellular organisation and repair and the preservation of gross anatomical perfection. Release of the first or anaerobic system from supervisory control by the second or aerobic sytem(s) permits the unlimited cell division which is the phenomenon known as cancer. The available circumstantial evidence suggests that this is not the result of nuclear or chromosomal defects or mutations but is due to direct irreversible sublethal damage to the cellular aerobic glucose metabolic system whilst the anaerobic system remains intact."}
{"id": "PMID:459965", "title": "Gastric cancer etiology: a biochemical hypothesis.", "content": "We have proposed a two-stage biochemical model for the etiology of \"intestinal\" gastric cancer. The model postulates that the gastric mucosal barrier is biochemically pierced as a result of chemical interactions between the mucoproteins and mucopolysaccharides of the barrier and ingested polysaccharrides (starches). This would allow the growth of gastric flora which could produce carcinogenic nitrosamines and/or nitrosamides. Observational and experimental evidence in favor of the model is provided. The model suggests various research initiatives, the results of which might provide the basis for biochemical and physiological methods for the prevention and/or treatment of gastric cancer. Various ways in which the model may be tested are also noted.", "contents": "Gastric cancer etiology: a biochemical hypothesis. We have proposed a two-stage biochemical model for the etiology of \"intestinal\" gastric cancer. The model postulates that the gastric mucosal barrier is biochemically pierced as a result of chemical interactions between the mucoproteins and mucopolysaccharides of the barrier and ingested polysaccharrides (starches). This would allow the growth of gastric flora which could produce carcinogenic nitrosamines and/or nitrosamides. Observational and experimental evidence in favor of the model is provided. The model suggests various research initiatives, the results of which might provide the basis for biochemical and physiological methods for the prevention and/or treatment of gastric cancer. Various ways in which the model may be tested are also noted."}
{"id": "PMID:459966", "title": "Childhood cancer and the SBLA syndrome.", "content": "Two nuclear families showing characteristics of the SBLA syndrome are described wherein progeny of breast cancer-affected mothers manifested early childhood malignant neoplasms. These observations have led us to postulate a novel type genetic-environmental interactive model which incorporates Knudsen's \"two-hit\" hypothesis as a partial explanation for the exceedingly early onset of cancer in the subject progeny. Given the assumption that the first hit was germinal with transfer of the deleterious SBLA gene at conception, we postulate that the second or somatic-hit occurred early on in utero. This may have involved a complex mechanism of one or more factors including tumor cell products, tumor specific antigens, immunosuppression, de-repressed oncogene, or an activated oncogenic virus via a transplacental communicable phenomenon. The testing of this new hypothesis dealing with carcinogenesis in the SBLA syndrome should employ immunologicalgenetic parameters concurrently in fetuses and mothers.", "contents": "Childhood cancer and the SBLA syndrome. Two nuclear families showing characteristics of the SBLA syndrome are described wherein progeny of breast cancer-affected mothers manifested early childhood malignant neoplasms. These observations have led us to postulate a novel type genetic-environmental interactive model which incorporates Knudsen's \"two-hit\" hypothesis as a partial explanation for the exceedingly early onset of cancer in the subject progeny. Given the assumption that the first hit was germinal with transfer of the deleterious SBLA gene at conception, we postulate that the second or somatic-hit occurred early on in utero. This may have involved a complex mechanism of one or more factors including tumor cell products, tumor specific antigens, immunosuppression, de-repressed oncogene, or an activated oncogenic virus via a transplacental communicable phenomenon. The testing of this new hypothesis dealing with carcinogenesis in the SBLA syndrome should employ immunologicalgenetic parameters concurrently in fetuses and mothers."}
{"id": "PMID:459967", "title": "Solar cycles and malignant melanoma.", "content": "There has been a sixfold increase in the incidence of malignant melanoma in the State of Connecticut during the past forty years. Superimposed on a steady incidence rise are cycles of markedly increased incidence rates which follow periods of maximum sunspot activity. We propose that the effect of sunspot cycles on human melanoma occurrence is mediated by modulation of stratospheric ozone and thus indirectly affects UV flux at the earth's surface. This hypothesis would predict a time lag in melanoma incidence cycles, relative to sunspot activity, with increasing distance from polar caps. This appears to be the case. The increase in melanoma incidence related to a given reduction in ozone depletion in this hypothesis, is in great excess of existing models relating anthropogenic ozone depletion and skin cancer.", "contents": "Solar cycles and malignant melanoma. There has been a sixfold increase in the incidence of malignant melanoma in the State of Connecticut during the past forty years. Superimposed on a steady incidence rise are cycles of markedly increased incidence rates which follow periods of maximum sunspot activity. We propose that the effect of sunspot cycles on human melanoma occurrence is mediated by modulation of stratospheric ozone and thus indirectly affects UV flux at the earth's surface. This hypothesis would predict a time lag in melanoma incidence cycles, relative to sunspot activity, with increasing distance from polar caps. This appears to be the case. The increase in melanoma incidence related to a given reduction in ozone depletion in this hypothesis, is in great excess of existing models relating anthropogenic ozone depletion and skin cancer."}
{"id": "PMID:459968", "title": "Proliferon: the functional unit of rapidly proliferating organs.", "content": "Continuously replicating organs are generally composed of several cell population types. These may be divided according to their function into two classes: 1. parenchyma: cells destined to perform a certain metabolic function peculiar to the organ under study, 2. supporting cell populations, consisting of fibroblasts and vascular supply always accompanied by nerve fibers. The kinetics of all the cells are highly coordinated. They all share one progenitor region. It is postulated that in this common progenitor region the two cell population classes are assembled into complex units denominated as proliferons. The proliferon starts its existence as a whole, matures as a whole and disintegrates at the organ periphery. It consists of four basic elements: parenchyma, connective tissue, blood vessels and nerve fibers. This model has been previously called upon to describe the kinetics of the rodent incisor tooth and the intestinal mucosa. It is assumed to be the elementary functional unit of all rapidly proliferating organs such as: skin, hair, endometrium, bone marrow, intestinal mucosa and rodent incisor.", "contents": "Proliferon: the functional unit of rapidly proliferating organs. Continuously replicating organs are generally composed of several cell population types. These may be divided according to their function into two classes: 1. parenchyma: cells destined to perform a certain metabolic function peculiar to the organ under study, 2. supporting cell populations, consisting of fibroblasts and vascular supply always accompanied by nerve fibers. The kinetics of all the cells are highly coordinated. They all share one progenitor region. It is postulated that in this common progenitor region the two cell population classes are assembled into complex units denominated as proliferons. The proliferon starts its existence as a whole, matures as a whole and disintegrates at the organ periphery. It consists of four basic elements: parenchyma, connective tissue, blood vessels and nerve fibers. This model has been previously called upon to describe the kinetics of the rodent incisor tooth and the intestinal mucosa. It is assumed to be the elementary functional unit of all rapidly proliferating organs such as: skin, hair, endometrium, bone marrow, intestinal mucosa and rodent incisor."}
{"id": "PMID:459969", "title": "Selectivity and rejectivity in cancer metastasis.", "content": "In cancer metastasis, \"selectivity\" denotes the formation of secondary tumor in an organ in such a manner that its localization pattern is peculiar. The opposite phenomenon should be recognized and called \"rejectivity\". In man, carcinomas appear to spread selectively to the umbilicus, whereas lymphomas seem to reject that site. In all probability, recognition of the twin theory of selectivity and rejectivity will stimulate research on cancer dissemination.", "contents": "Selectivity and rejectivity in cancer metastasis. In cancer metastasis, \"selectivity\" denotes the formation of secondary tumor in an organ in such a manner that its localization pattern is peculiar. The opposite phenomenon should be recognized and called \"rejectivity\". In man, carcinomas appear to spread selectively to the umbilicus, whereas lymphomas seem to reject that site. In all probability, recognition of the twin theory of selectivity and rejectivity will stimulate research on cancer dissemination."}
{"id": "PMID:459970", "title": "Is the role of the environment in carcinogenesis overestimated?", "content": "The dominant role of the physical and chemical environment in the development of cancer is challenged. Analyses of the etiology of skin, bladder, respiratory and gastric cancers are presented which demonstrate the considerable extent to which one's health status may modify the initiation and promotion of environmentally associated cancers. It is concluded that although environmental factors may initiate and/or promote 85 to 90 percent of all cancers this is misleading since it neglects the critical role of the individual's health status as a factor modifying carcinogenesis.", "contents": "Is the role of the environment in carcinogenesis overestimated? The dominant role of the physical and chemical environment in the development of cancer is challenged. Analyses of the etiology of skin, bladder, respiratory and gastric cancers are presented which demonstrate the considerable extent to which one's health status may modify the initiation and promotion of environmentally associated cancers. It is concluded that although environmental factors may initiate and/or promote 85 to 90 percent of all cancers this is misleading since it neglects the critical role of the individual's health status as a factor modifying carcinogenesis."}
{"id": "PMID:459971", "title": "On the aging of organisms and their cells.", "content": "An explanation of organismic ageing based on a limited division capacity of dividing cells is difficult to reconcile with much of the available data. The physiology of cells in ageing organisms tends, on the contrary, to suggest that organisms age as a result of degeneration in their non-dividing cell populations. Senescence in these non-mitotic cells resmebles the ageing of the non-dividing fraction of cell cultures clonally senescing, or maintained in long-term quiescence in vitro. As cultures of diploid human fibroblasts senesce there is an accumulation of non-dividing cells. Alterations in these post-mitotic cells can explain the senescent properties of late passage cultures. It is proposed that during the in vitro senescence of fibroblast cultures, cell ageing results from, as opposed to causes, the absence of mitosis. Cell ageing may primarily result from changes in the chromatin induced by the non-mitotic state.", "contents": "On the aging of organisms and their cells. An explanation of organismic ageing based on a limited division capacity of dividing cells is difficult to reconcile with much of the available data. The physiology of cells in ageing organisms tends, on the contrary, to suggest that organisms age as a result of degeneration in their non-dividing cell populations. Senescence in these non-mitotic cells resmebles the ageing of the non-dividing fraction of cell cultures clonally senescing, or maintained in long-term quiescence in vitro. As cultures of diploid human fibroblasts senesce there is an accumulation of non-dividing cells. Alterations in these post-mitotic cells can explain the senescent properties of late passage cultures. It is proposed that during the in vitro senescence of fibroblast cultures, cell ageing results from, as opposed to causes, the absence of mitosis. Cell ageing may primarily result from changes in the chromatin induced by the non-mitotic state."}
{"id": "PMID:459972", "title": "Intracellular hyperthermia. A biophysical approach to cancer treatment via intracellular temperature and biophysical alterations.", "content": "This paper introduces a new multi-disciplinary \"intracellular\" biophysical treatment of cancer. The basic concept uses locally induced heat energy after tumor phagocytosis of submicron particles whose composition permits magnetic excitation. The key to this process is the utilization of the cancer cell membrane to contain the energy within the cancer cell. Any magnetic or electric dipole contained within or introduced into the cell, or that is capable of being produced by an external field, can be used. Submicron particles are colloidally suspended, injected intravenously and are phagocytized by cancer cells. Application of an external high frequency or pulsed electromagnetic field then raises the particles' temperature thus generating intracellular heat in precise increments. This results in selective thermal destruction of cancer cells with little effect on normal cells. Experimental evidence is presented showing tumor cell destruction in spontaneous mammary tumors in Sprague Dawley rats. In addition, we suggest that certain biophysical properties are altered within the cancer cells and could be used to enhance this effect. Specific radioisotopes or tumor specific antibodies bound to particles or chemotherapeutic microspheres increase cancer cell sensitivity and affinity for these particles. This \"intracellular\" treatment of cancer has a wide potential range of applications.", "contents": "Intracellular hyperthermia. A biophysical approach to cancer treatment via intracellular temperature and biophysical alterations. This paper introduces a new multi-disciplinary \"intracellular\" biophysical treatment of cancer. The basic concept uses locally induced heat energy after tumor phagocytosis of submicron particles whose composition permits magnetic excitation. The key to this process is the utilization of the cancer cell membrane to contain the energy within the cancer cell. Any magnetic or electric dipole contained within or introduced into the cell, or that is capable of being produced by an external field, can be used. Submicron particles are colloidally suspended, injected intravenously and are phagocytized by cancer cells. Application of an external high frequency or pulsed electromagnetic field then raises the particles' temperature thus generating intracellular heat in precise increments. This results in selective thermal destruction of cancer cells with little effect on normal cells. Experimental evidence is presented showing tumor cell destruction in spontaneous mammary tumors in Sprague Dawley rats. In addition, we suggest that certain biophysical properties are altered within the cancer cells and could be used to enhance this effect. Specific radioisotopes or tumor specific antibodies bound to particles or chemotherapeutic microspheres increase cancer cell sensitivity and affinity for these particles. This \"intracellular\" treatment of cancer has a wide potential range of applications."}
{"id": "PMID:459973", "title": "The influence of ambient ozone on the incidence of bone fractures especially among the elderly.", "content": "Elevated levels of breatheable ozone will reduce the amount of UV radiation in the range of 280-305 nm reaching the surface of earth. This range of UV converts the provitamin 7-dehydrocholesterol to vitamin D, within the human. Since most typical diets contain low levels of vitamin D, the role of UV related vitamin D synthesis in the skin is considered to provide very important contributions to the total vitamin D content of the blood. Thus, elevated levels of ambient ozone should result in a decreased level of vitamin D synthesis which may be expressed as an increase in the incidence of histological osteomalacia and ultimately bone fractures, especially among the elderly.", "contents": "The influence of ambient ozone on the incidence of bone fractures especially among the elderly. Elevated levels of breatheable ozone will reduce the amount of UV radiation in the range of 280-305 nm reaching the surface of earth. This range of UV converts the provitamin 7-dehydrocholesterol to vitamin D, within the human. Since most typical diets contain low levels of vitamin D, the role of UV related vitamin D synthesis in the skin is considered to provide very important contributions to the total vitamin D content of the blood. Thus, elevated levels of ambient ozone should result in a decreased level of vitamin D synthesis which may be expressed as an increase in the incidence of histological osteomalacia and ultimately bone fractures, especially among the elderly."}
{"id": "PMID:459974", "title": "Ischaemic heart disease-an hypothesis to integrate the role of insulin, fibre and sucrose.", "content": "The theory that a high dietary cholesterol and saturated fat intake has an aetiological role in coronary artery disease is inadequate. So are those theories based upon insulin, upon refined carbohydrate and upon dietary sucrose. An alternate hypothesis is presented. Central to it is prolonged hyperinsulin-aemia. This, acting synergistically with the changes in lipide metabolism secondary to the effects of dietary fibre and sucrose, is crucial to atherogenesis. There is abundant evidence for an association of insulin and vascular changes, both in vitro and in epidemiological studies. Laboratory and epidemiological evidence also exists for an effect of fibre and soluble carbohydrate on cholesterol synthesis and excretion, both as itself and as bile acid. A scheme is presented to demonstrate these complex inter-relationships and their role in vascular diseases.", "contents": "Ischaemic heart disease-an hypothesis to integrate the role of insulin, fibre and sucrose. The theory that a high dietary cholesterol and saturated fat intake has an aetiological role in coronary artery disease is inadequate. So are those theories based upon insulin, upon refined carbohydrate and upon dietary sucrose. An alternate hypothesis is presented. Central to it is prolonged hyperinsulin-aemia. This, acting synergistically with the changes in lipide metabolism secondary to the effects of dietary fibre and sucrose, is crucial to atherogenesis. There is abundant evidence for an association of insulin and vascular changes, both in vitro and in epidemiological studies. Laboratory and epidemiological evidence also exists for an effect of fibre and soluble carbohydrate on cholesterol synthesis and excretion, both as itself and as bile acid. A scheme is presented to demonstrate these complex inter-relationships and their role in vascular diseases."}
{"id": "PMID:459975", "title": "Toward establishing scientific credibility in acupuncture research.", "content": "While the effectiveness of acupuncture has been established clinically, research methods and tools which are basic for establishing objective scientific credibility in medicine have been a problem in acupuncture research. Research methods developed in other areas and reported in the literature are presented which could, if applied to acupuncture research, contribute toward establishing scientific credibility in medicine.", "contents": "Toward establishing scientific credibility in acupuncture research. While the effectiveness of acupuncture has been established clinically, research methods and tools which are basic for establishing objective scientific credibility in medicine have been a problem in acupuncture research. Research methods developed in other areas and reported in the literature are presented which could, if applied to acupuncture research, contribute toward establishing scientific credibility in medicine."}
{"id": "PMID:459976", "title": "Psychosomatic disease and acupuncture.", "content": "The medical philosophy implied by a \"mind-body unity\" hypothesis is outlined and the type of therapy appropriate to the stage of development of disease is discussed; special reference is made to the place of acupuncture within a modern medical program. Starting from the premise that mind is the primary causal force determining neural states, it is concluded that the highest level medical art would be \"psychosomatic psychoanalysis.\" The next highest form of therapy would deal directly with nerve impulses (acupuncture, etc.), followed by drug therapies directed at intra- and extracellular fluid composition, hormone macromolecule manipulation, and finally minor and major surgery.", "contents": "Psychosomatic disease and acupuncture. The medical philosophy implied by a \"mind-body unity\" hypothesis is outlined and the type of therapy appropriate to the stage of development of disease is discussed; special reference is made to the place of acupuncture within a modern medical program. Starting from the premise that mind is the primary causal force determining neural states, it is concluded that the highest level medical art would be \"psychosomatic psychoanalysis.\" The next highest form of therapy would deal directly with nerve impulses (acupuncture, etc.), followed by drug therapies directed at intra- and extracellular fluid composition, hormone macromolecule manipulation, and finally minor and major surgery."}
{"id": "PMID:459977", "title": "Limitation of resistance as a parameter by which to characterize epithelia that actively transport ions.", "content": "It is theoretically inappropriate to characterize an actively transporting epithelim by \"resistance\" alone which is correctly applied only to passive circuit elements. Rather, such epithelia (if they actively transport sodium) required, as a minimum, characterization by an active circuit element parameter such as voltage (E Na), current (I Na) or power (WNa) in some configuration with resistances. Recent experimental studies of epithelia which actively transport sodium have omitted consideration of the active circuit element and attributed all measured changes observed to \"resistance\" changes in the epithelium as the transepithelial sodium gradient is altered. It is suggested that the observed changes in voltage/current ratio could be consequences of changes in the electrical behavior of the active circuit element of such epithelia. It may be biologically impossible to suppress all electromotive forces in epithelia to measure the truly passive characteristics of the epithelim without destroying tissue viability. The actual methods used to date for measurement of \"resistance\" in epithelia consist of perturbing signals which might alter the electrical behavior of an active element such as an \"ion pump\"; the observed changes in voltage to current ratio observed in such experiments can be better explained by a change in the active ion pump rather than by changes in passive epithelial \"resistance\".", "contents": "Limitation of resistance as a parameter by which to characterize epithelia that actively transport ions. It is theoretically inappropriate to characterize an actively transporting epithelim by \"resistance\" alone which is correctly applied only to passive circuit elements. Rather, such epithelia (if they actively transport sodium) required, as a minimum, characterization by an active circuit element parameter such as voltage (E Na), current (I Na) or power (WNa) in some configuration with resistances. Recent experimental studies of epithelia which actively transport sodium have omitted consideration of the active circuit element and attributed all measured changes observed to \"resistance\" changes in the epithelium as the transepithelial sodium gradient is altered. It is suggested that the observed changes in voltage/current ratio could be consequences of changes in the electrical behavior of the active circuit element of such epithelia. It may be biologically impossible to suppress all electromotive forces in epithelia to measure the truly passive characteristics of the epithelim without destroying tissue viability. The actual methods used to date for measurement of \"resistance\" in epithelia consist of perturbing signals which might alter the electrical behavior of an active element such as an \"ion pump\"; the observed changes in voltage to current ratio observed in such experiments can be better explained by a change in the active ion pump rather than by changes in passive epithelial \"resistance\"."}
{"id": "PMID:459978", "title": "Potential for therapeutic gain similar to pions by daily combinations of neutrons and low-LET radiations.", "content": "Biological studies with negative pi mesons (pions) at the Los Alamos Meson Physics Facility (LAMPF) have shown a relatively constant reduction of shoulder of the survival curve for single cell cultures and relatively constant reduction in the no response region for multicellular tumor spheroids (MTS) with little change in sensitivity (or slope), in both single dose and fractionated experiments. In clinical studies, a trend toward therapeutic gain for pions has been demonstrated in patients treated at LAMPF, with marked, rapid turmor regression for relatively mild acute normal tissue injury and no untoward effects observed over 7 to 24 months. Since the pion beams used therapeutically at LAMPF are characterized by a small amount of high-linear-energy-transfer (LET) radiation (in the range 10 to 20%), it is hypothesized that pion radiation as compared to x-rays increased the differential tolerance to sublethal injury in favor of normal tissues, resulting in therapeutic gain. It is further proposed that the near simultaneous application of high-LET radiation (such as neutrons) and low-LET radiation (such as x-rays) might create a bilogical situation similar to that of pion treatment, potentially resulting in therapeutic gain, although without the added benefit of dose localization obtainable with pions.", "contents": "Potential for therapeutic gain similar to pions by daily combinations of neutrons and low-LET radiations. Biological studies with negative pi mesons (pions) at the Los Alamos Meson Physics Facility (LAMPF) have shown a relatively constant reduction of shoulder of the survival curve for single cell cultures and relatively constant reduction in the no response region for multicellular tumor spheroids (MTS) with little change in sensitivity (or slope), in both single dose and fractionated experiments. In clinical studies, a trend toward therapeutic gain for pions has been demonstrated in patients treated at LAMPF, with marked, rapid turmor regression for relatively mild acute normal tissue injury and no untoward effects observed over 7 to 24 months. Since the pion beams used therapeutically at LAMPF are characterized by a small amount of high-linear-energy-transfer (LET) radiation (in the range 10 to 20%), it is hypothesized that pion radiation as compared to x-rays increased the differential tolerance to sublethal injury in favor of normal tissues, resulting in therapeutic gain. It is further proposed that the near simultaneous application of high-LET radiation (such as neutrons) and low-LET radiation (such as x-rays) might create a bilogical situation similar to that of pion treatment, potentially resulting in therapeutic gain, although without the added benefit of dose localization obtainable with pions."}
{"id": "PMID:459980", "title": "Etiology of neural tube defect in man--Do teratogens play any part? Evidence from sex ratios.", "content": "Neural tube defect (NTD) is more common among spontaneously aborted fetuses than in infants born in the third trimester, but there is no direct evidence that NTD-affected conceptuses, presenting at these two different gestational ages, are components of a single disease process. Evidence for homology is here presented in an analysis of the sex ratios of spontaneously and therapeutically aborted NTD-affected fetuses, and of sex ratios differing with variations in prevalence of NTD-affected infants. If it could be shown conclusively that NTD is a single disease process, with an inverse relationship between components expressed early and late in gestation, there would be implications for the ascertainment of families at risk for NTD, and for the search for environmental factors potentially involved in the causation of NTD.", "contents": "Etiology of neural tube defect in man--Do teratogens play any part? Evidence from sex ratios. Neural tube defect (NTD) is more common among spontaneously aborted fetuses than in infants born in the third trimester, but there is no direct evidence that NTD-affected conceptuses, presenting at these two different gestational ages, are components of a single disease process. Evidence for homology is here presented in an analysis of the sex ratios of spontaneously and therapeutically aborted NTD-affected fetuses, and of sex ratios differing with variations in prevalence of NTD-affected infants. If it could be shown conclusively that NTD is a single disease process, with an inverse relationship between components expressed early and late in gestation, there would be implications for the ascertainment of families at risk for NTD, and for the search for environmental factors potentially involved in the causation of NTD."}
{"id": "PMID:459981", "title": "Pathogenesis of cystic fibrosis: possible importance of alteration in epithelial surface topography.", "content": "As the pathogenesis in cystic fibrosis (CF) has been considered to lie in a disturbance in the behavior of secretions, particularly mucins, from exocrine glands, it has been proposed that an alteration in surface topography (form, number or distribution of microvilli or blebs) of epithelial cells lining ducts could impair distribution leading to retention of mucus leading to the clinical phenotype. Scanning electronmicroscopy of human epithelial suspension cultures now offers an opportunity to evaluate the role of plasmalemma topography in CF.", "contents": "Pathogenesis of cystic fibrosis: possible importance of alteration in epithelial surface topography. As the pathogenesis in cystic fibrosis (CF) has been considered to lie in a disturbance in the behavior of secretions, particularly mucins, from exocrine glands, it has been proposed that an alteration in surface topography (form, number or distribution of microvilli or blebs) of epithelial cells lining ducts could impair distribution leading to retention of mucus leading to the clinical phenotype. Scanning electronmicroscopy of human epithelial suspension cultures now offers an opportunity to evaluate the role of plasmalemma topography in CF."}
{"id": "PMID:459982", "title": "Is kernicterus due to inhibition of brain hexose-monophosphate shunt activity by bilirubin?", "content": "Degradation of biogenic amines in the central nervous system is accomplished by the monoamine oxidase system. The by-products of this reaction, hydrogen peroxide and amino aldehydes, are toxic to neurones. Detoxification of these by-products is normally mediated by generation of NADPH via the hexosemonophosphate (HMP) shunt. It is proposed that inhibition of the HMP shunt by unconjugated bilirubin leads to the toxic accumulation of hydrogen peroxide and aldehydes.", "contents": "Is kernicterus due to inhibition of brain hexose-monophosphate shunt activity by bilirubin? Degradation of biogenic amines in the central nervous system is accomplished by the monoamine oxidase system. The by-products of this reaction, hydrogen peroxide and amino aldehydes, are toxic to neurones. Detoxification of these by-products is normally mediated by generation of NADPH via the hexosemonophosphate (HMP) shunt. It is proposed that inhibition of the HMP shunt by unconjugated bilirubin leads to the toxic accumulation of hydrogen peroxide and aldehydes."}
{"id": "PMID:459983", "title": "The therapeutic effect of hemodialysis on schizophrenia: comments and further possibilities.", "content": "Intermittent hemodialysis was recently found to have a therapeutic effect on schizophrenia, pointing to biochemical disturbances and possibly to renal malfunction as the cause of some forms of mental disorder. This paper points out that intermittent hemodialysis is not equally effective for the elimination of all solutes in the plasma. It can dispose of significant quantities of substances for which the normal urine/plasma concentration ratio is small, not of substances which the kidney concentrates in the urine by a large factor. It is further pointed out that for substances with a small urine/plasma concentration ratio thermogenic sweating could also constitute an effective means of disposal and its use is suggested as possible alternative to hemodialysis.", "contents": "The therapeutic effect of hemodialysis on schizophrenia: comments and further possibilities. Intermittent hemodialysis was recently found to have a therapeutic effect on schizophrenia, pointing to biochemical disturbances and possibly to renal malfunction as the cause of some forms of mental disorder. This paper points out that intermittent hemodialysis is not equally effective for the elimination of all solutes in the plasma. It can dispose of significant quantities of substances for which the normal urine/plasma concentration ratio is small, not of substances which the kidney concentrates in the urine by a large factor. It is further pointed out that for substances with a small urine/plasma concentration ratio thermogenic sweating could also constitute an effective means of disposal and its use is suggested as possible alternative to hemodialysis."}
{"id": "PMID:459984", "title": "Change and stability in cellular organisms.", "content": "The living cell is characterized by both its potential for change and its tendency towards a stable state. The interaction between the DNA information system and the metabolic steady state is described. A model based on this interaction leads to the conclusions that change can only be initiated from the environment but that the direction of any change is determined by the cell.", "contents": "Change and stability in cellular organisms. The living cell is characterized by both its potential for change and its tendency towards a stable state. The interaction between the DNA information system and the metabolic steady state is described. A model based on this interaction leads to the conclusions that change can only be initiated from the environment but that the direction of any change is determined by the cell."}
{"id": "PMID:459985", "title": "Hyperhidrosis and the sympatho-adrenal system.", "content": "Some disorders in which excessive sweating, hyperhidrosis, is a symptom are also characterized by increased sympatho-adrenal activity. Such disorders are hypotension, hypoglycemia, pheochromocytoma and hyperthyroidism. Sweat glands are controlled by a cholinergic innervation but can also be stimulated by adrenergic agents whose effects can be blocked by both alpha- and beta-receptor blocking drugs. An adrenergic innervation has also been demonstrated. There is evidence that the adrenergic component of sweating particularly secretion of the adrenal medulla, is responsible for the enhancement of sweating during exercise but not for the hyperhidrosis present in these disorders since sweating in these circumstances can be effectively blocked by cholinergic blocking compounds. Cutaneous vasoconstriction due to elevated plasma catecholamines reduces the rate of evaporation of sweat and allows sweat to accumulate on the skin. It is suggested that in the case of hypotension and insulin hypoglycemia sweating results from general sympathetic stimulation and that adrenal medullary hormones are not an essential component of the response. Hyperhidrosis in pheochromocytoma may be due to central activation of heat loss mechanisms resulting from the passage of plasma catecholamines across the blood-brain barrier combined with increased thermogenesis and cutaneous vasoconstriction. The hyperhidrosis of hyperthyroidism is probably due to increased thermogenesis.", "contents": "Hyperhidrosis and the sympatho-adrenal system. Some disorders in which excessive sweating, hyperhidrosis, is a symptom are also characterized by increased sympatho-adrenal activity. Such disorders are hypotension, hypoglycemia, pheochromocytoma and hyperthyroidism. Sweat glands are controlled by a cholinergic innervation but can also be stimulated by adrenergic agents whose effects can be blocked by both alpha- and beta-receptor blocking drugs. An adrenergic innervation has also been demonstrated. There is evidence that the adrenergic component of sweating particularly secretion of the adrenal medulla, is responsible for the enhancement of sweating during exercise but not for the hyperhidrosis present in these disorders since sweating in these circumstances can be effectively blocked by cholinergic blocking compounds. Cutaneous vasoconstriction due to elevated plasma catecholamines reduces the rate of evaporation of sweat and allows sweat to accumulate on the skin. It is suggested that in the case of hypotension and insulin hypoglycemia sweating results from general sympathetic stimulation and that adrenal medullary hormones are not an essential component of the response. Hyperhidrosis in pheochromocytoma may be due to central activation of heat loss mechanisms resulting from the passage of plasma catecholamines across the blood-brain barrier combined with increased thermogenesis and cutaneous vasoconstriction. The hyperhidrosis of hyperthyroidism is probably due to increased thermogenesis."}
{"id": "PMID:459986", "title": "Heart disease, cancer and vehicle travel.", "content": "A new concept as to the cause of heart disease, cancers and diabetes is put forward in this paper. It is established that there is a mathematical relationship between motor vehicle use, from 1920 to 1975, and deaths due to the abovementioned diseases. This relationship has been established by an extensive statistical study of vehicle usage and deaths due to these diseases.", "contents": "Heart disease, cancer and vehicle travel. A new concept as to the cause of heart disease, cancers and diabetes is put forward in this paper. It is established that there is a mathematical relationship between motor vehicle use, from 1920 to 1975, and deaths due to the abovementioned diseases. This relationship has been established by an extensive statistical study of vehicle usage and deaths due to these diseases."}
{"id": "PMID:459987", "title": "\"Fatigue on rest\" and associated symptoms (headache, vertigo, blurred vision, nausea, tension and irritability) due to locally asymptomatic, unerupted, impacted teeth.", "content": "\"Fatigue on Rest\", headache, vertigo and the feeling of loss of balance, blurred vision, nausea, tension and irritability, were found to be prevalent amongst patients who had locally asymptomatic, unerupted impacted teeth. A comparative pressure sign was developed, which, when positive, confirmed the relationship between the impacted teeth and the medical symptoms. Removal of the impactions resulted in the alleviation of the symptoms. Stress and psychogenic factors are considered as trigger mechanisms, rather than as basic causes of the symptoms.", "contents": "\"Fatigue on rest\" and associated symptoms (headache, vertigo, blurred vision, nausea, tension and irritability) due to locally asymptomatic, unerupted, impacted teeth. \"Fatigue on Rest\", headache, vertigo and the feeling of loss of balance, blurred vision, nausea, tension and irritability, were found to be prevalent amongst patients who had locally asymptomatic, unerupted impacted teeth. A comparative pressure sign was developed, which, when positive, confirmed the relationship between the impacted teeth and the medical symptoms. Removal of the impactions resulted in the alleviation of the symptoms. Stress and psychogenic factors are considered as trigger mechanisms, rather than as basic causes of the symptoms."}
{"id": "PMID:459988", "title": "Metastasis as a beneficial process.", "content": "The most important message to be found in the \"Fourth report on end results in cancer\" in the U.S., states that with the progression of cancer, its force of mortality declines. This has been reexamined in the present study. The declining force of mortality implies that as the disease advances the chances of the average patient to survive, improve. Since in the cancer patient all vital functions gradually deteriorate, and the only process gaining with time is his tumor load, one has to consider the possibility that the improving chances of the cancer patient could be linked with the amount of tumor mass in his body. These ideas are illustrated by the following example. Cancer could result from a gradual loss of a vital tissue product 'A', to be replaced by an analogous tissue product 'B' which is of embryonal origin. In the adult, 'B' is produced by stem cells which gradually adapt to the loss of the primary product and increase in number. 'B' is less efficient than 'A' to meet the necessary vital functions. Its deficiency in quality is however augmented by quantity. In order to keep up with the increasing demand, the stem cells proliferate and spread throughout the organism where each metastasis continues to secrete 'B'. The penalty inflicted by this compensatory mechanism is relatively high. Some metastases hit vital functions, and the increasing tumor load depletes the available energy sources. The net effect however is beneficial since without metastasis the organism would have succumbed to the disease in its earliest stage.", "contents": "Metastasis as a beneficial process. The most important message to be found in the \"Fourth report on end results in cancer\" in the U.S., states that with the progression of cancer, its force of mortality declines. This has been reexamined in the present study. The declining force of mortality implies that as the disease advances the chances of the average patient to survive, improve. Since in the cancer patient all vital functions gradually deteriorate, and the only process gaining with time is his tumor load, one has to consider the possibility that the improving chances of the cancer patient could be linked with the amount of tumor mass in his body. These ideas are illustrated by the following example. Cancer could result from a gradual loss of a vital tissue product 'A', to be replaced by an analogous tissue product 'B' which is of embryonal origin. In the adult, 'B' is produced by stem cells which gradually adapt to the loss of the primary product and increase in number. 'B' is less efficient than 'A' to meet the necessary vital functions. Its deficiency in quality is however augmented by quantity. In order to keep up with the increasing demand, the stem cells proliferate and spread throughout the organism where each metastasis continues to secrete 'B'. The penalty inflicted by this compensatory mechanism is relatively high. Some metastases hit vital functions, and the increasing tumor load depletes the available energy sources. The net effect however is beneficial since without metastasis the organism would have succumbed to the disease in its earliest stage."}
{"id": "PMID:459989", "title": "Behavioral effects of cyclic changes in serotonin during the human menstrual cycle.", "content": "Many cyclic changes during the menstrual cycle (temperature, depression, motor activity, pain sensitivity, etc.) are closely paralleled by changes in brain serotonin level. These changes, in turn, are associated with peripheral hormone levels which are comparatively regular and easily measured. Their measurement may be useful both in predicting behavior and in accounting for atypical menstrual-related behavior.", "contents": "Behavioral effects of cyclic changes in serotonin during the human menstrual cycle. Many cyclic changes during the menstrual cycle (temperature, depression, motor activity, pain sensitivity, etc.) are closely paralleled by changes in brain serotonin level. These changes, in turn, are associated with peripheral hormone levels which are comparatively regular and easily measured. Their measurement may be useful both in predicting behavior and in accounting for atypical menstrual-related behavior."}
{"id": "PMID:459991", "title": "Basic principles of cellular organization.", "content": "The hypothesis is put forward that the division of the animal body into three germ layers represents not only histological, but also functional specialization, the ectoderm taking over functions through which the animal is in contact with, acted upon or reacts to the external world, the entoderm metabolism and the mesoderm architectural and mechanical organization. This is assumed to mean that the tasks of life have been divided into three large function domains at an early stage of multicellular evolution, each germ layer taking over one domain and developing largely independently of the other two in the further course of phylogeny. The germ layers are autonomous in their own spheres, giving the body, in effect, a tripartite government. The potentiality of conceptual thought is inherent in all germ layers, reaching a high state of development in the entoderm and mesoderm at a much earlier stage of development than in the ectoderm. The animal body, as a whole, is in control of all its biologic activities; it is a self-designing, self-developing and self-perpetuating entity. The control of cellular organization is a mesodermal task.", "contents": "Basic principles of cellular organization. The hypothesis is put forward that the division of the animal body into three germ layers represents not only histological, but also functional specialization, the ectoderm taking over functions through which the animal is in contact with, acted upon or reacts to the external world, the entoderm metabolism and the mesoderm architectural and mechanical organization. This is assumed to mean that the tasks of life have been divided into three large function domains at an early stage of multicellular evolution, each germ layer taking over one domain and developing largely independently of the other two in the further course of phylogeny. The germ layers are autonomous in their own spheres, giving the body, in effect, a tripartite government. The potentiality of conceptual thought is inherent in all germ layers, reaching a high state of development in the entoderm and mesoderm at a much earlier stage of development than in the ectoderm. The animal body, as a whole, is in control of all its biologic activities; it is a self-designing, self-developing and self-perpetuating entity. The control of cellular organization is a mesodermal task."}
{"id": "PMID:459992", "title": "The mechanism of coronary artery spasm: roles of oxygen, prostaglandins, sex hormones and smoking.", "content": "A reduced oxygen supply to the heart causes coronary vasodilatation in the first instance. But if the hypoxia is severe or prolonged, the dilatation passes off and coronary vasospasm develops leading to a vicious circle with a further reduction of myocardial oxygenation. The spasm is associated with increased outflow of prostaglandin (PG)-like material and can be prevented or reversed by inhibitors of PG synthesis such as indomethacin or antagonists of PG action such as chloroquine. The spasm does not appear to be caused by thromboxane (TX) A2 since selective inhibitors of TXA2 synthesis enhance the hypoxic spasm and by themselves can cause spasm even in oxygenated hearts. The mechanism may be related to loss of negative feedback control of the PG pathway by TXA2. Oxygen may enhance TXA2 production and reduce formation of vasoconstrictor PGs, while smoking, because of the formation of carboxyhaemoglobin, may have the opposite effect. Oestradiol and testosterone do not influence the hypoxic spasm but progesterone at physiological concentrations blocks it completely. Progesterone may be the protective female hormone and the increased susceptibility to myocardial infarction in women on oral contraceptives may be related to reduced formation of endogenous progesterone.", "contents": "The mechanism of coronary artery spasm: roles of oxygen, prostaglandins, sex hormones and smoking. A reduced oxygen supply to the heart causes coronary vasodilatation in the first instance. But if the hypoxia is severe or prolonged, the dilatation passes off and coronary vasospasm develops leading to a vicious circle with a further reduction of myocardial oxygenation. The spasm is associated with increased outflow of prostaglandin (PG)-like material and can be prevented or reversed by inhibitors of PG synthesis such as indomethacin or antagonists of PG action such as chloroquine. The spasm does not appear to be caused by thromboxane (TX) A2 since selective inhibitors of TXA2 synthesis enhance the hypoxic spasm and by themselves can cause spasm even in oxygenated hearts. The mechanism may be related to loss of negative feedback control of the PG pathway by TXA2. Oxygen may enhance TXA2 production and reduce formation of vasoconstrictor PGs, while smoking, because of the formation of carboxyhaemoglobin, may have the opposite effect. Oestradiol and testosterone do not influence the hypoxic spasm but progesterone at physiological concentrations blocks it completely. Progesterone may be the protective female hormone and the increased susceptibility to myocardial infarction in women on oral contraceptives may be related to reduced formation of endogenous progesterone."}
{"id": "PMID:459993", "title": "An oculo-cardiac hypothesis of sudden unexpected death in infants.", "content": "The oculo-cardiac hypothesis presented here draws attention to the evidence for links between Sudden Unexpected Death in Infants (SUD) - known in America as the Sudden Infant Death Syndrome, also as \"cot death\" or \"crib death\" - and the vagally mediated reflex slowing of the heart beat following pressure on the eye. A brief review is made of epidemiologic findings on SUD, including the fact that around 9000 infant fatalities per year are attributed to SUD in the U.S.A. alone. Evidence for excessive vagal discharge as a likely factor in SUD is discussed. The nature of the oculo-cardiac reflex discovered by Aschner is described, with particular attention to those factors which appear to make specific categories of sleeping infant more vulnerable to cardiac arrest in circumstances where this reflex is elicited. The factors include prematurity and an existing electrical instability of the heart. Regarding the manner in which such an oculo-cardiac reflex might in practice be set off, it is suggested that this could occur if the infant slept in such a position that the weight of the head could press the eye against any irregular surface which might exist on the mattress, or any object which was resting on the mattress. Evidence is cited for SUD infants being in fact more likely than controls to sleep in such a position. Children with exophthalmos would be more at risk in this respect. It is stressed that all this evidence is already published, but has not previously been drawn together to show the likelihood of a link between Sudden Dath and the oculo-cardiac reflex.", "contents": "An oculo-cardiac hypothesis of sudden unexpected death in infants. The oculo-cardiac hypothesis presented here draws attention to the evidence for links between Sudden Unexpected Death in Infants (SUD) - known in America as the Sudden Infant Death Syndrome, also as \"cot death\" or \"crib death\" - and the vagally mediated reflex slowing of the heart beat following pressure on the eye. A brief review is made of epidemiologic findings on SUD, including the fact that around 9000 infant fatalities per year are attributed to SUD in the U.S.A. alone. Evidence for excessive vagal discharge as a likely factor in SUD is discussed. The nature of the oculo-cardiac reflex discovered by Aschner is described, with particular attention to those factors which appear to make specific categories of sleeping infant more vulnerable to cardiac arrest in circumstances where this reflex is elicited. The factors include prematurity and an existing electrical instability of the heart. Regarding the manner in which such an oculo-cardiac reflex might in practice be set off, it is suggested that this could occur if the infant slept in such a position that the weight of the head could press the eye against any irregular surface which might exist on the mattress, or any object which was resting on the mattress. Evidence is cited for SUD infants being in fact more likely than controls to sleep in such a position. Children with exophthalmos would be more at risk in this respect. It is stressed that all this evidence is already published, but has not previously been drawn together to show the likelihood of a link between Sudden Dath and the oculo-cardiac reflex."}
{"id": "PMID:459994", "title": "Glaucoma hypothesis: application of the law of Laplace.", "content": "The objective of the proposed research is to design a test for anticipating glaucoma damage. In this project, the author is utilizing a mathematical equation defined by Laplace's law as applied to a hollow organ. Eye wall stress should be evaluated concomitantly with the intraocular pressure, in order to define the susceptibility of the eye to develop glaucoma. The idea presented in this paper allows for better understanding of different factors responsible for the pathogenesis of open angle glaucoma, of low pressure glaucoma, and of ocular hypertension. It allows one to predict how a given eye will react to a given pressure, that is, whether damage will occur or not. It might answer the question whether to treat right away, or wait and follow. It explains the higher prevalence of glaucoma in myopic eyes. It outlines easy, noninvasive, inexpensive preventive measures, which do not require new and costly facilities. The next step, in further elaboration of this project, should probably be to evaluate the relationship between the ocular pulse and pressure curve. This will be discussed and presented in the future.", "contents": "Glaucoma hypothesis: application of the law of Laplace. The objective of the proposed research is to design a test for anticipating glaucoma damage. In this project, the author is utilizing a mathematical equation defined by Laplace's law as applied to a hollow organ. Eye wall stress should be evaluated concomitantly with the intraocular pressure, in order to define the susceptibility of the eye to develop glaucoma. The idea presented in this paper allows for better understanding of different factors responsible for the pathogenesis of open angle glaucoma, of low pressure glaucoma, and of ocular hypertension. It allows one to predict how a given eye will react to a given pressure, that is, whether damage will occur or not. It might answer the question whether to treat right away, or wait and follow. It explains the higher prevalence of glaucoma in myopic eyes. It outlines easy, noninvasive, inexpensive preventive measures, which do not require new and costly facilities. The next step, in further elaboration of this project, should probably be to evaluate the relationship between the ocular pulse and pressure curve. This will be discussed and presented in the future."}
{"id": "PMID:459995", "title": "Sources of energy for the brain and susceptibility to audiogenic seizures.", "content": "The onset of susceptibility to audiogenic seizures (AGS) coincides with the draining of the ear canal at about 14 to 16 days of age. This is also when the mouse brain has almost attained its maximal size and weight, and also about the time of weaning from the dam's high-fat milk to the beginning of dietary self-sufficiency. During suckling, the brain is primarily dependent on ketone-body utilization as a source for brain energy; weaned mice use glucose. It is suggested that in AGS-prone mice, there may be a developmental lag in the onset of a sufficient rate of glycolysis in brain to provide adequate immediately available energy reserves to last through a brief period of an external-stimulus-induced large energy expediture until energy repletion processes can begin. As a results, ATP levels might fall below an hypothesized lower limit to subserve organized neural activity in some inhibitory area of the brain, resulting in the onset of an AGS.", "contents": "Sources of energy for the brain and susceptibility to audiogenic seizures. The onset of susceptibility to audiogenic seizures (AGS) coincides with the draining of the ear canal at about 14 to 16 days of age. This is also when the mouse brain has almost attained its maximal size and weight, and also about the time of weaning from the dam's high-fat milk to the beginning of dietary self-sufficiency. During suckling, the brain is primarily dependent on ketone-body utilization as a source for brain energy; weaned mice use glucose. It is suggested that in AGS-prone mice, there may be a developmental lag in the onset of a sufficient rate of glycolysis in brain to provide adequate immediately available energy reserves to last through a brief period of an external-stimulus-induced large energy expediture until energy repletion processes can begin. As a results, ATP levels might fall below an hypothesized lower limit to subserve organized neural activity in some inhibitory area of the brain, resulting in the onset of an AGS."}
{"id": "PMID:459996", "title": "Can elevated levels of copper in drinking water precipitate aucte hemolysis in G-6-PD deficient individuals?", "content": "The hypothesis that elevated levels of copper in drinking water may be a factor in the precipitation of acute hemolysis in those with a G-6-PD deficiency in their erythrocytes is developed. The present U.S. EPA drinking water standards do not have a primary (or human health) standard for copper but only a secondary standard which is designed to prevent an unacceptable taste. Because of the widespread occurrence of elevated levels of copper in drinking water in areas where the water is corrosive and the households have copper piping, it is recommended that environmental health regulatory agencies develop a risk assessment concerning the effects of copper on those high risk groups who are predisposed to develop a copper induced hemolysis.", "contents": "Can elevated levels of copper in drinking water precipitate aucte hemolysis in G-6-PD deficient individuals? The hypothesis that elevated levels of copper in drinking water may be a factor in the precipitation of acute hemolysis in those with a G-6-PD deficiency in their erythrocytes is developed. The present U.S. EPA drinking water standards do not have a primary (or human health) standard for copper but only a secondary standard which is designed to prevent an unacceptable taste. Because of the widespread occurrence of elevated levels of copper in drinking water in areas where the water is corrosive and the households have copper piping, it is recommended that environmental health regulatory agencies develop a risk assessment concerning the effects of copper on those high risk groups who are predisposed to develop a copper induced hemolysis."}
{"id": "PMID:459998", "title": "Hypercalcaemia in adult hospital population: comparison of inpatients of 1966 with those of 1976.", "content": "During 1976, inpatients with hypercalcaemia at The Royal Melbourne Hospital were identified from the worksheets of the Department of Biochemistry and compared with those discovered in a similar study in 1966. In 1966, the estimation of plasma calcium level had to be ordered specifically, whereas in 1976, for technical reasons, the measurement of plasma calcium level was frequently performed when not requested. Despite a six-fold increase in plasma calcium in the rate of detection of hypercalcaemia. In both studies, malignant disease was the most common casue of hypercalcaemia, but primary common cause of hypercalcaemia, but primary hyperparathyroidism was found slightly more frequently in 1976. This study suggests that screening inpatients for hypercalcaemia is of little value.", "contents": "Hypercalcaemia in adult hospital population: comparison of inpatients of 1966 with those of 1976. During 1976, inpatients with hypercalcaemia at The Royal Melbourne Hospital were identified from the worksheets of the Department of Biochemistry and compared with those discovered in a similar study in 1966. In 1966, the estimation of plasma calcium level had to be ordered specifically, whereas in 1976, for technical reasons, the measurement of plasma calcium level was frequently performed when not requested. Despite a six-fold increase in plasma calcium in the rate of detection of hypercalcaemia. In both studies, malignant disease was the most common casue of hypercalcaemia, but primary common cause of hypercalcaemia, but primary hyperparathyroidism was found slightly more frequently in 1976. This study suggests that screening inpatients for hypercalcaemia is of little value."}
{"id": "PMID:459999", "title": "Effect of smoking on the recurrence of malignant melanoma.", "content": "Smoking has been recently shown to lead to aberrant immunological function in man. Since immunological host resistance is important in the natural history of human malignant melanoma, it was the object of this study to determine the effects of smoking on the course of this disease. Investigations on 1908 patients with malignant melanoma indicated that smoking renders men significantly more susceptible to developing metastases. The proportion of men smokers free of disease five years after melanoma diagnosis was significantly lower than that of men non-smokers. An effect of smoking similar to that recorded in men was not evident in women with malignant melanoma.", "contents": "Effect of smoking on the recurrence of malignant melanoma. Smoking has been recently shown to lead to aberrant immunological function in man. Since immunological host resistance is important in the natural history of human malignant melanoma, it was the object of this study to determine the effects of smoking on the course of this disease. Investigations on 1908 patients with malignant melanoma indicated that smoking renders men significantly more susceptible to developing metastases. The proportion of men smokers free of disease five years after melanoma diagnosis was significantly lower than that of men non-smokers. An effect of smoking similar to that recorded in men was not evident in women with malignant melanoma."}
{"id": "PMID:460000", "title": "Premature rupture of the membranes.", "content": "In a consecutive series of 93 patients with premature rupture of the membranes at 20 to 34 weeks of gestation, the perinatal mortality was 23.7%. One-quater of the deaths were due to lethal congenital abnormalities, 18% were due to intrauterine infection, and 36% resulted from severe respiratory distress syndrome (RDS). Corticosteroid therapy increased the risk of incompetence, and did not reduce the incidence or severity of RDS. Almost one-third of the patients were delivered within 48 hours of membrane rupture; however, short-term treatment with salbutamol was able to delay the delivery for at least six days in 5 of the 13 patients to whom it was given. As postponement of delivery for days or weeks after the membranes have ruptured reduces the incidence and severity of Rds, this therapy may well have a place in the treatment of this condition.", "contents": "Premature rupture of the membranes. In a consecutive series of 93 patients with premature rupture of the membranes at 20 to 34 weeks of gestation, the perinatal mortality was 23.7%. One-quater of the deaths were due to lethal congenital abnormalities, 18% were due to intrauterine infection, and 36% resulted from severe respiratory distress syndrome (RDS). Corticosteroid therapy increased the risk of incompetence, and did not reduce the incidence or severity of RDS. Almost one-third of the patients were delivered within 48 hours of membrane rupture; however, short-term treatment with salbutamol was able to delay the delivery for at least six days in 5 of the 13 patients to whom it was given. As postponement of delivery for days or weeks after the membranes have ruptured reduces the incidence and severity of Rds, this therapy may well have a place in the treatment of this condition."}
{"id": "PMID:460001", "title": "Premature labour.", "content": "Intravenously administered salbutamol inhibited premature labour for at least 48 hours in 49 of 76 patients (64%), but was more effective (89%) when the cervical dilatation was less than 2 cm, and no apparent cause for the premature labour was evident. Even when premature labour complicated multiple pregnancy, hydramnios, or followed a small antepartum haemorrhage, suppression of labour could often be achieved for at least 48 hours, thus following the administration of betamethasone or the transfer of the patient to a hospital with intensive care facilities for both mother and child. Treatment with betamethasone, in addition to salbutamol, was associated with a less severe form of respiratory distress syndrome, and did not increase the risk of maternal or fetal infection, except in patients with cervical incompetence when 46.2% of such patients were infected.", "contents": "Premature labour. Intravenously administered salbutamol inhibited premature labour for at least 48 hours in 49 of 76 patients (64%), but was more effective (89%) when the cervical dilatation was less than 2 cm, and no apparent cause for the premature labour was evident. Even when premature labour complicated multiple pregnancy, hydramnios, or followed a small antepartum haemorrhage, suppression of labour could often be achieved for at least 48 hours, thus following the administration of betamethasone or the transfer of the patient to a hospital with intensive care facilities for both mother and child. Treatment with betamethasone, in addition to salbutamol, was associated with a less severe form of respiratory distress syndrome, and did not increase the risk of maternal or fetal infection, except in patients with cervical incompetence when 46.2% of such patients were infected."}
{"id": "PMID:460020", "title": "Child abuse in the 24 hours after psychotherapy sessions.", "content": "Six cases in which, unknown to the therapist, psychotherapy apparently initiated or exacerbated child abuse are reviewed. Once the connection between therapy and child abuse was recognized and pointed out to the patients, further therapy led to a significant clinical improvement. The necessity for additional support, such as is offered by Prevention, is emphasized.", "contents": "Child abuse in the 24 hours after psychotherapy sessions. Six cases in which, unknown to the therapist, psychotherapy apparently initiated or exacerbated child abuse are reviewed. Once the connection between therapy and child abuse was recognized and pointed out to the patients, further therapy led to a significant clinical improvement. The necessity for additional support, such as is offered by Prevention, is emphasized."}
{"id": "PMID:460021", "title": "Fitting below-elbow prostheses which convey a sense of touch.", "content": "A myoelectrically controlled electric hand has been modified so that the gripping force between thumb and index finger may be measured. The measurement is used to control a sensory feedback device applied to the surface of the skin within the socket of the prosthesis informing the wearer of the strength of grip exerted. Two people have been fitted with this prosthesis and they report that the sensory feedback gives them reassurance when using the arm. To date there has not been any noticeable adverse tissue reponse from the applied sensory stimulus.", "contents": "Fitting below-elbow prostheses which convey a sense of touch. A myoelectrically controlled electric hand has been modified so that the gripping force between thumb and index finger may be measured. The measurement is used to control a sensory feedback device applied to the surface of the skin within the socket of the prosthesis informing the wearer of the strength of grip exerted. Two people have been fitted with this prosthesis and they report that the sensory feedback gives them reassurance when using the arm. To date there has not been any noticeable adverse tissue reponse from the applied sensory stimulus."}
{"id": "PMID:460022", "title": "Bed occupancy by diabetic patients.", "content": "The Royal Newcastle Hospital Diabetic Education and Stabilization Centre was instituted primarily to improve diabetics' understanding of their disease and its everyday management. Simultaneously with a five-day education course, stabilization if insulin-dependent diabetics was undertaken on an outpatient basis. In order to disseminate diabetic education as widely as possible, trained nurses from near and far were included in each course. It is believed that these nurses will be able to offer good advice to diabetics who have no other source of reliable information. By actively attempting to reduce hospital bed occupancy by diabetics there has been a reduction of 1400 bed days per year. It is recommended that similar centres be instituted throughout Australia.", "contents": "Bed occupancy by diabetic patients. The Royal Newcastle Hospital Diabetic Education and Stabilization Centre was instituted primarily to improve diabetics' understanding of their disease and its everyday management. Simultaneously with a five-day education course, stabilization if insulin-dependent diabetics was undertaken on an outpatient basis. In order to disseminate diabetic education as widely as possible, trained nurses from near and far were included in each course. It is believed that these nurses will be able to offer good advice to diabetics who have no other source of reliable information. By actively attempting to reduce hospital bed occupancy by diabetics there has been a reduction of 1400 bed days per year. It is recommended that similar centres be instituted throughout Australia."}
{"id": "PMID:460023", "title": "Pelvic phleboliths: a study of 528 melanesian and white patients in papua new guinea.", "content": "The prevalence of pelvic phleboliths was compared in 359 Melanasian patients and 169 white patients in Port Moresby, Papua New Guinea, and was found to be 12% and 51% respectively. The number and the size of phleboliths were also greater in white patients. These findings are similar to the data available from other parts of the world. The lower prevalence of pelvic phleboliths (and deep vein thrombosis) in Papua New Guineans may be related to their high fibre content diet, to their more active fibrinolytic system, or to both.", "contents": "Pelvic phleboliths: a study of 528 melanesian and white patients in papua new guinea. The prevalence of pelvic phleboliths was compared in 359 Melanasian patients and 169 white patients in Port Moresby, Papua New Guinea, and was found to be 12% and 51% respectively. The number and the size of phleboliths were also greater in white patients. These findings are similar to the data available from other parts of the world. The lower prevalence of pelvic phleboliths (and deep vein thrombosis) in Papua New Guineans may be related to their high fibre content diet, to their more active fibrinolytic system, or to both."}
{"id": "PMID:460025", "title": "[Digoxin concentrations in plasma and tissue. A postmortal investigation (author's transl)].", "content": "Postmortal tissue digoxin concentration and prefinal and postmortal plasma digoxin concentrations from 9 patients were determined using the 125J-digoxin radioimmunoassay. The concentration of digoxin in the myocardium of the left ventricle was 61.44 +/- 45.17 ng/g. In the right ventricle the concentration was 36.78 +/- 33.11 ng/g. Higher digoxin concentrations were found in the right atrium (34.93 +/- 21.86 ng/g) than in the left atrium (25.43 +/- 14.57 ng/g). In plasma samples taken after death approximately 62% higher digoxin concentrations were found than in samples of patients in prefinal state. Histological examination revealed positive correlation between in the high varying digoxin concentrations and the pathological conditions of the tissue structure.", "contents": "[Digoxin concentrations in plasma and tissue. A postmortal investigation (author's transl)]. Postmortal tissue digoxin concentration and prefinal and postmortal plasma digoxin concentrations from 9 patients were determined using the 125J-digoxin radioimmunoassay. The concentration of digoxin in the myocardium of the left ventricle was 61.44 +/- 45.17 ng/g. In the right ventricle the concentration was 36.78 +/- 33.11 ng/g. Higher digoxin concentrations were found in the right atrium (34.93 +/- 21.86 ng/g) than in the left atrium (25.43 +/- 14.57 ng/g). In plasma samples taken after death approximately 62% higher digoxin concentrations were found than in samples of patients in prefinal state. Histological examination revealed positive correlation between in the high varying digoxin concentrations and the pathological conditions of the tissue structure."}
{"id": "PMID:460026", "title": "[Studies on the diuretic effects of etozolin (Elkapin) in heart failure - a comparison with the loop diuretic agent furosemide (author's transl)].", "content": "In 115 randomized patients with left and/or right ventricular failure, the effect of the new diuretic Etozolin (800 mg p.o.) (n = 55) is compared with that of the loop diuretic Furosemide (80 mg p.o.) (n = 60). 1. The increased diuresis after Etozolin remains constant during the entire trial period. Furosemid initially induces a more intense diuresis. 2. Analysis of the action profile shows that the higher daily urinary output following Furosemide is due to a more intense diuresis in the first fractions of the day. The action of Etozolin is more constant and lasts into the evening. 3. Both substances reduce body weight to the same extent. 4. Heart rate and arterial blood pressure decline significantly during trial. 5. Etozolin induces lesser electrolyte elimination than Furosemide in the initial phase of the trial. Potassium elimination values, in particular, remain below Na+ and Cl- elimination values for both substances. 6. During the trial period both substances had no significant effect on blood and liver values, serum electrolytes, creatinine, urea and uric acid. Etozolin may be classified as a diuretic similar to a thiazide derivative in its 24-h profile but behaves like a loop diuretic in its diuretic effect.", "contents": "[Studies on the diuretic effects of etozolin (Elkapin) in heart failure - a comparison with the loop diuretic agent furosemide (author's transl)]. In 115 randomized patients with left and/or right ventricular failure, the effect of the new diuretic Etozolin (800 mg p.o.) (n = 55) is compared with that of the loop diuretic Furosemide (80 mg p.o.) (n = 60). 1. The increased diuresis after Etozolin remains constant during the entire trial period. Furosemid initially induces a more intense diuresis. 2. Analysis of the action profile shows that the higher daily urinary output following Furosemide is due to a more intense diuresis in the first fractions of the day. The action of Etozolin is more constant and lasts into the evening. 3. Both substances reduce body weight to the same extent. 4. Heart rate and arterial blood pressure decline significantly during trial. 5. Etozolin induces lesser electrolyte elimination than Furosemide in the initial phase of the trial. Potassium elimination values, in particular, remain below Na+ and Cl- elimination values for both substances. 6. During the trial period both substances had no significant effect on blood and liver values, serum electrolytes, creatinine, urea and uric acid. Etozolin may be classified as a diuretic similar to a thiazide derivative in its 24-h profile but behaves like a loop diuretic in its diuretic effect."}
{"id": "PMID:460027", "title": "[Complication during the dipyridamole-test (author's transl)].", "content": "Performing the dipyridamole-test for evaluating coronary artery disease a man at the age of 57 years developed after application of 15 mg dipyridamole intravenously angina pectoris. For removal of angina pectoris 240 mg aminophylline were injected, after what bradycardia and finally cardiac arrest occurred. Resuscitation was successful. Possible reasons and some precautions were discussed.", "contents": "[Complication during the dipyridamole-test (author's transl)]. Performing the dipyridamole-test for evaluating coronary artery disease a man at the age of 57 years developed after application of 15 mg dipyridamole intravenously angina pectoris. For removal of angina pectoris 240 mg aminophylline were injected, after what bradycardia and finally cardiac arrest occurred. Resuscitation was successful. Possible reasons and some precautions were discussed."}
{"id": "PMID:460030", "title": "[Replacement of mitral and aortic valve of a 12 years old boy (author's transl)].", "content": "Report of the case of a 12 years old boy, who because of acquired mitral valve insufficiency (grades II--III), after an illness of virus-carditis, became, in the first instance, a Bjoerk-Shiley-Ventil M27 prosthetic valvular replacement, and after 3 months, in one sitting, correction of a paravalvular leakage and prosthetic valvular replacement of the insufficient aortic valve with a Bjoerk-Shiley A23. The boy was well rehabilitated one year after the last operation. This is supported by the similar results of the echocardiographic, the ergometric and the pulmonary function tests carried out. The size of the replaced prosthetic valve makes the long time prognosis favourable. Double-valvular prosthetic replacement in children was hitherto carried out predominantly as a result of rheumatic diseases. The rheumatic process as well as congenital valvular malformation could surely be excluded in our patient so that the virus-process seems to be the most likely cause of the valvular defect.", "contents": "[Replacement of mitral and aortic valve of a 12 years old boy (author's transl)]. Report of the case of a 12 years old boy, who because of acquired mitral valve insufficiency (grades II--III), after an illness of virus-carditis, became, in the first instance, a Bjoerk-Shiley-Ventil M27 prosthetic valvular replacement, and after 3 months, in one sitting, correction of a paravalvular leakage and prosthetic valvular replacement of the insufficient aortic valve with a Bjoerk-Shiley A23. The boy was well rehabilitated one year after the last operation. This is supported by the similar results of the echocardiographic, the ergometric and the pulmonary function tests carried out. The size of the replaced prosthetic valve makes the long time prognosis favourable. Double-valvular prosthetic replacement in children was hitherto carried out predominantly as a result of rheumatic diseases. The rheumatic process as well as congenital valvular malformation could surely be excluded in our patient so that the virus-process seems to be the most likely cause of the valvular defect."}
{"id": "PMID:460031", "title": "[Rapid atrial stimulation in the treatment of atrial flutter (author's transl)].", "content": "Study on 35 patients suffering from atrial flutter, in which rapid atrial stimulation was made 40 times. Stimulation-catheters were introduced only by control of the intracardiac ecg without x-ray control. Sinus rhythm was restored in 31 cases, in 17 cases intermediate atrial fibrillation of varying length of duration. In 5 cases atrial fibrillation with normofrequent ventricular action was achieved. Appreciable complications were not seen. Essential advantages of the rapid atrial stimulation are the additional diagnostic information by the intracardiac ecg, the dispensable narcosis, the independence of a simultaneous medicamentous treatment, and the use in cases of sick sinus syndrome, too.", "contents": "[Rapid atrial stimulation in the treatment of atrial flutter (author's transl)]. Study on 35 patients suffering from atrial flutter, in which rapid atrial stimulation was made 40 times. Stimulation-catheters were introduced only by control of the intracardiac ecg without x-ray control. Sinus rhythm was restored in 31 cases, in 17 cases intermediate atrial fibrillation of varying length of duration. In 5 cases atrial fibrillation with normofrequent ventricular action was achieved. Appreciable complications were not seen. Essential advantages of the rapid atrial stimulation are the additional diagnostic information by the intracardiac ecg, the dispensable narcosis, the independence of a simultaneous medicamentous treatment, and the use in cases of sick sinus syndrome, too."}
{"id": "PMID:460034", "title": "[Bronchial abnormalities in a clinic for respiratory diseases (author's transl)].", "content": "The frequency in bronchial abnormalities is generally underestimated. In patients of a clinic for respiratory diseases however 3% were found in those having had a bronchoscopy, a figure which has up to now not been mentioned in the literature. After discussing originating theories and classifying possibilities the observations are tabulated, and some of them introduced as representative examples. In the discussion it is pointed out, that the abnormalities apparently represent a locus minoris resistentiae, since pathological changes are often found in these areas.", "contents": "[Bronchial abnormalities in a clinic for respiratory diseases (author's transl)]. The frequency in bronchial abnormalities is generally underestimated. In patients of a clinic for respiratory diseases however 3% were found in those having had a bronchoscopy, a figure which has up to now not been mentioned in the literature. After discussing originating theories and classifying possibilities the observations are tabulated, and some of them introduced as representative examples. In the discussion it is pointed out, that the abnormalities apparently represent a locus minoris resistentiae, since pathological changes are often found in these areas."}
{"id": "PMID:460035", "title": "[Early detection of bronchial cancer (author's transl)].", "content": "Compared with the results of a retrospective study (1954) of early detecting bronchial cancer, our present investigations showed, that females are relatively more concerned than formerly, that the climax of frequency has removed for about ten years to the aged, that more non-smokers are concerned than formerly (16% to 3.5% respectively) and that the time between manifestation of the first symptoms and admittance to hospital has shortened remarkably (one month 26%, three months 33% = 59% compared with 28.3%). In four patients the disease has been discovered by x-ray mass-screening, two of them were nevertheless admitted to hospital too late. Whereas the value of tomography has been established, the results of bronchoscopy ameliorated. Only one patient could be operated: perhaps the shift of age for one decade and a certain negative selection may be responsible for these disappointing results. The x-ray-examination in 2 levels must be performed in every adult male patient, even if bronchopulmonal symptoms do not exist. Moreover, exposed persons (e.g. strong smokers) must be controlled by x-ray examination in regular intervals. It is in this sense, that we see the scope of x-ray mass-screening.", "contents": "[Early detection of bronchial cancer (author's transl)]. Compared with the results of a retrospective study (1954) of early detecting bronchial cancer, our present investigations showed, that females are relatively more concerned than formerly, that the climax of frequency has removed for about ten years to the aged, that more non-smokers are concerned than formerly (16% to 3.5% respectively) and that the time between manifestation of the first symptoms and admittance to hospital has shortened remarkably (one month 26%, three months 33% = 59% compared with 28.3%). In four patients the disease has been discovered by x-ray mass-screening, two of them were nevertheless admitted to hospital too late. Whereas the value of tomography has been established, the results of bronchoscopy ameliorated. Only one patient could be operated: perhaps the shift of age for one decade and a certain negative selection may be responsible for these disappointing results. The x-ray-examination in 2 levels must be performed in every adult male patient, even if bronchopulmonal symptoms do not exist. Moreover, exposed persons (e.g. strong smokers) must be controlled by x-ray examination in regular intervals. It is in this sense, that we see the scope of x-ray mass-screening."}
{"id": "PMID:460036", "title": "[Thyrotoxicosis and sarcoidosis of the thyroid and the lung (author's transl)].", "content": "Clinical and pathological studies indicate, that sarcoidosis rarely affects the thyroid gland. The diagnosis of sarcoidosis in the lung and in the thyroid has been established by biopsies taken during surgery in a case of a 53 year old female. The manifestation of thyrotoxicosis in sarcoidosis-involvement of the thyroid has been analysed and compared with the 15 patients exhibiting sarcoidosis-involvement of the thyroid and thyrotoxicosis described in the literature.", "contents": "[Thyrotoxicosis and sarcoidosis of the thyroid and the lung (author's transl)]. Clinical and pathological studies indicate, that sarcoidosis rarely affects the thyroid gland. The diagnosis of sarcoidosis in the lung and in the thyroid has been established by biopsies taken during surgery in a case of a 53 year old female. The manifestation of thyrotoxicosis in sarcoidosis-involvement of the thyroid has been analysed and compared with the 15 patients exhibiting sarcoidosis-involvement of the thyroid and thyrotoxicosis described in the literature."}
{"id": "PMID:460038", "title": "[Transport of infants at risk (author's transl)].", "content": "We report upon a two-years experience with transportation of prematures, newborn infants and children up to two years of age. Most patients were prematures and newborn infants (1.38% of lifeborns in 1976 and 1.83% in 1977) out of the region of Aachen. They were transferred to the intensive care unit of the children's hospital (primary transport). 21.2% of them died. The mortality rate of all neonates, treated in the children's hospital, was reduced by 3.68% in comparison to the period before organized transportation system three years ago. The number of deceased was diminished by more than 40%. Some of the newborns remained in the maternity hospitals after first providing by the \"transport-team\". We also transported children up to two years of age with the mobile intensive care unit. Diagnostical and therapeutical procedures in other departments of the hospital of Aachen and other specialized centres became possible because of diminished risk.", "contents": "[Transport of infants at risk (author's transl)]. We report upon a two-years experience with transportation of prematures, newborn infants and children up to two years of age. Most patients were prematures and newborn infants (1.38% of lifeborns in 1976 and 1.83% in 1977) out of the region of Aachen. They were transferred to the intensive care unit of the children's hospital (primary transport). 21.2% of them died. The mortality rate of all neonates, treated in the children's hospital, was reduced by 3.68% in comparison to the period before organized transportation system three years ago. The number of deceased was diminished by more than 40%. Some of the newborns remained in the maternity hospitals after first providing by the \"transport-team\". We also transported children up to two years of age with the mobile intensive care unit. Diagnostical and therapeutical procedures in other departments of the hospital of Aachen and other specialized centres became possible because of diminished risk."}
{"id": "PMID:460039", "title": "[D-penicillamine in various diseases in childhood (author's transl)].", "content": "D-pencillamine, a stable not physiological amino acid, has a manifold mode of action. Of special importance there is its influence on the collagen-metabolism, the gelose of heavy metals and the effect on immunologic processes. The use of D-penicillamine is possible in different diseases, such as Wilson's syndrome, collagenoses of diverse kinds, especially the rheumatoid arthritis, further chronic hepatitis and lung fibrosis. In this paper we report about 52 children, who were treated with D-penicillamine. The biggest group presented chronic liver diseases in 24 patients and rheumaoid arthritis in 21 patients. The therapy was carried out for a longer time, in some cases over years. The dose varied from 15 to 35 mg/kg of body weight. The number of side-effects was lower in children than in adults. They were more frequent in the group of collagenoses than in the group of liver diseases. Whether later on liver damages will occur is not predictable by the pediatrician. The results were excellent for the chronic active hepatitis; we can recommend D-penicillamine for such affections. Also for the rheumatoid arthritis we could partially obtain good successes, but not as convincing as in liver-diseases.", "contents": "[D-penicillamine in various diseases in childhood (author's transl)]. D-pencillamine, a stable not physiological amino acid, has a manifold mode of action. Of special importance there is its influence on the collagen-metabolism, the gelose of heavy metals and the effect on immunologic processes. The use of D-penicillamine is possible in different diseases, such as Wilson's syndrome, collagenoses of diverse kinds, especially the rheumatoid arthritis, further chronic hepatitis and lung fibrosis. In this paper we report about 52 children, who were treated with D-penicillamine. The biggest group presented chronic liver diseases in 24 patients and rheumaoid arthritis in 21 patients. The therapy was carried out for a longer time, in some cases over years. The dose varied from 15 to 35 mg/kg of body weight. The number of side-effects was lower in children than in adults. They were more frequent in the group of collagenoses than in the group of liver diseases. Whether later on liver damages will occur is not predictable by the pediatrician. The results were excellent for the chronic active hepatitis; we can recommend D-penicillamine for such affections. Also for the rheumatoid arthritis we could partially obtain good successes, but not as convincing as in liver-diseases."}
{"id": "PMID:460044", "title": "Chromosomes and causation of human cancer and leukemia: XXXVI. The 14q+ anomaly in an American Burkitt lymphoma and its value in the definition of lymphoproliferative disorders.", "content": "A case of a 10-year-old boy with American Burkitt lymphoma is presented in whom a 14q+ due to t(8;14)(q23;q32) was shown to exist in the ascitic lymphoma cells. This appears to be the first demonstration of such a translocation in uncultured material. In addition, another translocation involving the X chromosome, hitherto not observed in Burkitt tumors, was demonstrated. The karyotypic findings have been related to the cytogenetic experience in Burkitt and other lymphomas, with emphasis being put on the importance of the 14q+ anomaly in lymphoproliferative diseases.", "contents": "Chromosomes and causation of human cancer and leukemia: XXXVI. The 14q+ anomaly in an American Burkitt lymphoma and its value in the definition of lymphoproliferative disorders. A case of a 10-year-old boy with American Burkitt lymphoma is presented in whom a 14q+ due to t(8;14)(q23;q32) was shown to exist in the ascitic lymphoma cells. This appears to be the first demonstration of such a translocation in uncultured material. In addition, another translocation involving the X chromosome, hitherto not observed in Burkitt tumors, was demonstrated. The karyotypic findings have been related to the cytogenetic experience in Burkitt and other lymphomas, with emphasis being put on the importance of the 14q+ anomaly in lymphoproliferative diseases."}
{"id": "PMID:460046", "title": "Serial studies of serum dopamine-B-hydroxylase and urinary vanillylmandelic and homovanillic acids in neuroblastoma.", "content": "Recent reports have suggested that elevations of serum dopamine-B-hydroxylase (DBH) activity may correlate with diagnosis in neuroblastoma patients excreting vanillylmandelic acid (VMA). We have serially studied serum DBH and urinary homovanillic acid (HVA) and VMA excretion during the disease course of five patients with neuroblastoma. DBH activities did not indicate clinical course, therapies, or prognosis. Dilution studies revealed an age-related alteration in DBH effectors and they suggest that these may be different in neuroblastoma.", "contents": "Serial studies of serum dopamine-B-hydroxylase and urinary vanillylmandelic and homovanillic acids in neuroblastoma. Recent reports have suggested that elevations of serum dopamine-B-hydroxylase (DBH) activity may correlate with diagnosis in neuroblastoma patients excreting vanillylmandelic acid (VMA). We have serially studied serum DBH and urinary homovanillic acid (HVA) and VMA excretion during the disease course of five patients with neuroblastoma. DBH activities did not indicate clinical course, therapies, or prognosis. Dilution studies revealed an age-related alteration in DBH effectors and they suggest that these may be different in neuroblastoma."}
{"id": "PMID:460060", "title": "Analysis of the significance of scattered radiation in reduced dose mammography, including magnification effects, scatter suppression, and focal spot and detector blurring.", "content": "A systems analysis of reduced dose mammography with scatter removal has quantitatively specified the relationships between the several parameters in mammography systems. While the results are only preliminary in the sense that they need to be proven experimentally, they do seem to provide a good feel for the nature of the important trade-offs. The major uncertainties in the analysis probably have to do with the formal equivalence, that is implicit in the aperture theory, between large area and detail contrast, as well as the output versus focal spot relationship that was adopted. Also, there are important questions about specifying motion unsharpness as a function of exposure time in mammography examinations.", "contents": "Analysis of the significance of scattered radiation in reduced dose mammography, including magnification effects, scatter suppression, and focal spot and detector blurring. A systems analysis of reduced dose mammography with scatter removal has quantitatively specified the relationships between the several parameters in mammography systems. While the results are only preliminary in the sense that they need to be proven experimentally, they do seem to provide a good feel for the nature of the important trade-offs. The major uncertainties in the analysis probably have to do with the formal equivalence, that is implicit in the aperture theory, between large area and detail contrast, as well as the output versus focal spot relationship that was adopted. Also, there are important questions about specifying motion unsharpness as a function of exposure time in mammography examinations."}
{"id": "PMID:460059", "title": "Effect of arm orientation on bone mineral mass and bone width measured using the Cameron-Sorenson technique.", "content": "Bone mineral content and bone width were measured as the distal and midshaft locations in the right radius and ulna with the arm in different orientations. The values of mineral content, width, and the ratio of bone mineral content divided by width, determined at each bone site were compared to establish the effect of orientation. Seven orientations were studied, two of which involved rotations of the forearm and five of which involved rotations of the upper arm about the axis of the forearm. Rotation of the forearm was found to affect the values of one or more of the measured quantities at all of the sites. Orientation of the upper arm was found to affect the values of all quantities at the midshaft radius, but not to affect the values of any of the quantities at the other sites.", "contents": "Effect of arm orientation on bone mineral mass and bone width measured using the Cameron-Sorenson technique. Bone mineral content and bone width were measured as the distal and midshaft locations in the right radius and ulna with the arm in different orientations. The values of mineral content, width, and the ratio of bone mineral content divided by width, determined at each bone site were compared to establish the effect of orientation. Seven orientations were studied, two of which involved rotations of the forearm and five of which involved rotations of the upper arm about the axis of the forearm. Rotation of the forearm was found to affect the values of one or more of the measured quantities at all of the sites. Orientation of the upper arm was found to affect the values of all quantities at the midshaft radius, but not to affect the values of any of the quantities at the other sites."}
{"id": "PMID:460058", "title": "Stereo-photogrammetry for the determination of patient surface geometry.", "content": "A stereo-photographic system has been developed with which surface contours of a human subject may be obtained rapidly and objectively. Nonmetric cameras are used and the results are obtained from direct measurements of the photographs. Software has been developed for interpretation of the photographic data with the assistance of a small computer and desk-top digitizer such as those routinely used in radiotherapy treatment planning. With the system the coordinates of a point on the subject may be determined with an accuracy of +/- 1-2 mm.", "contents": "Stereo-photogrammetry for the determination of patient surface geometry. A stereo-photographic system has been developed with which surface contours of a human subject may be obtained rapidly and objectively. Nonmetric cameras are used and the results are obtained from direct measurements of the photographs. Software has been developed for interpretation of the photographic data with the assistance of a small computer and desk-top digitizer such as those routinely used in radiotherapy treatment planning. With the system the coordinates of a point on the subject may be determined with an accuracy of +/- 1-2 mm."}
{"id": "PMID:460064", "title": "Attenuation in Lipowitz's metal of x rays produced at 2, 4, 10, and 18 MV and gamma rays from cobalt-60.", "content": "Attenuation in Lipowitz's metal of x rays produced at 2, 4, 10, and 18 MV and gamma rays from cobalt 60 were measured to the 1% level with a narrow beam geometry. Blocks made from standard styrofoam mold (7.6 cm thick) would reduce these primary beams by 95% to 99%.", "contents": "Attenuation in Lipowitz's metal of x rays produced at 2, 4, 10, and 18 MV and gamma rays from cobalt-60. Attenuation in Lipowitz's metal of x rays produced at 2, 4, 10, and 18 MV and gamma rays from cobalt 60 were measured to the 1% level with a narrow beam geometry. Blocks made from standard styrofoam mold (7.6 cm thick) would reduce these primary beams by 95% to 99%."}
{"id": "PMID:460061", "title": "Quantum fluctuations in radiographic screen-film systems.", "content": "With the recent introduction of fast rare-earth screens into the market, the question of noise and how it influences image quality becomes particularly important. In this paper a compact formula involving familiar and measurable quantities is derived for the specification of the noise arising from the spatial fluctuations of the x-ray quanta absorbed in the screens. Experimental evidence in support of this concept is also given.", "contents": "Quantum fluctuations in radiographic screen-film systems. With the recent introduction of fast rare-earth screens into the market, the question of noise and how it influences image quality becomes particularly important. In this paper a compact formula involving familiar and measurable quantities is derived for the specification of the noise arising from the spatial fluctuations of the x-ray quanta absorbed in the screens. Experimental evidence in support of this concept is also given."}
{"id": "PMID:460065", "title": "Computer generated scatter dose distributions for 6-MV radiotherapy photon beams.", "content": "The time required for accurate computer dose distributions is of major concern in radiation therapy. Isodose distributions were obtained by calculating the primary and scatter radiation reaching a point. A description of the primary dose distribution, especially at the edge of the beam, requires the calculation of the dose on a finely spaced grid. However, the scatter dose distributions can be described on a much coarser grid. The primary and scatter dose distribution calculations were separated into two computer subroutines which permitted choosing the appropriate grid spacing for each dose component. A more rational partition of the computing time for the primary and scatter dose was obtained. In addition, the separation of the calculation of the scatter dose from the total dose allows one to examine the calculated scatter dose distribution as the radial and angular increments used in the Clarkson-Cunningham summation are varied. Accurate scatter dose distributions were generated with a tenfold time reduction by optimization of grid spacing and radial and angular increments.", "contents": "Computer generated scatter dose distributions for 6-MV radiotherapy photon beams. The time required for accurate computer dose distributions is of major concern in radiation therapy. Isodose distributions were obtained by calculating the primary and scatter radiation reaching a point. A description of the primary dose distribution, especially at the edge of the beam, requires the calculation of the dose on a finely spaced grid. However, the scatter dose distributions can be described on a much coarser grid. The primary and scatter dose distribution calculations were separated into two computer subroutines which permitted choosing the appropriate grid spacing for each dose component. A more rational partition of the computing time for the primary and scatter dose was obtained. In addition, the separation of the calculation of the scatter dose from the total dose allows one to examine the calculated scatter dose distribution as the radial and angular increments used in the Clarkson-Cunningham summation are varied. Accurate scatter dose distributions were generated with a tenfold time reduction by optimization of grid spacing and radial and angular increments."}
{"id": "PMID:460062", "title": "Radiographic quality, tube potential, and patient dose.", "content": "Measurements were made to determine if there was an optimal kVp that would maximize radiographic contrast for various materials while minimizing average patient dose. Radiographs were made of a 17-cm-thick plexiglass phantom (to simulate a typical abdomen) with added regions of plexiglass, hydroxyapatite, and iodine with peak kilovoltages from 40 to 150 kVp and 2 mm A1 added filtration. Radiographs were also made of a 4-cm-thick plexiglass phantom to simulate a typical breast, with added regions of plexiglass, hydroxyapatite, and fat at a peak kilovoltage from 35 to 100 kVp and with 1 mm added filtration. For the thicker phantom it was found that the contrast per average absorbed dose for the added plexiglass, hydroxyapatite, and iodine increased by about 3, 2, and 0.4, respectively, when going from 50 to 150 kVp. Contrast was measured relative to a reference region on the phantom. Similar data for the thinner phantom showed an increase of about 35% in contrast per average absorbed dose for plexiglass and fat but a decrease by about 30% for hydroxyapatite as the kVp changes from 35 to 100 kVp.", "contents": "Radiographic quality, tube potential, and patient dose. Measurements were made to determine if there was an optimal kVp that would maximize radiographic contrast for various materials while minimizing average patient dose. Radiographs were made of a 17-cm-thick plexiglass phantom (to simulate a typical abdomen) with added regions of plexiglass, hydroxyapatite, and iodine with peak kilovoltages from 40 to 150 kVp and 2 mm A1 added filtration. Radiographs were also made of a 4-cm-thick plexiglass phantom to simulate a typical breast, with added regions of plexiglass, hydroxyapatite, and fat at a peak kilovoltage from 35 to 100 kVp and with 1 mm added filtration. For the thicker phantom it was found that the contrast per average absorbed dose for the added plexiglass, hydroxyapatite, and iodine increased by about 3, 2, and 0.4, respectively, when going from 50 to 150 kVp. Contrast was measured relative to a reference region on the phantom. Similar data for the thinner phantom showed an increase of about 35% in contrast per average absorbed dose for plexiglass and fat but a decrease by about 30% for hydroxyapatite as the kVp changes from 35 to 100 kVp."}
{"id": "PMID:460063", "title": "Relative measurements of fast neutron contamination in 18-MV photon beams from two linear accelerators and a betatron.", "content": "Fast neutron contamination in photon beams in the 20 MV range have been reported in recent years. In order to determine if the variations were due mainly to differences in measurement procedures, or inherent in the design of the accelerators, three different 18-MV (BJR) photon beams were compared using identical analytical techniques. The units studied were a Philips SL/75-20 and a Siemens Mevatron-20 linear accelerators and a Schimadzu betatron. Gamma spectroscopy of an activated aluminum foil was the method used. By comparing the relative amounts of neutron contamination, errors associated with absolute measurements such as detector efficiency and differences in activation foils were eliminated. Fast neutron contaminations per rad of x rays in a ratio of 6.7:3.7:1 were found for the Philips, Schimadzu and Siemens accelerators, respectively.", "contents": "Relative measurements of fast neutron contamination in 18-MV photon beams from two linear accelerators and a betatron. Fast neutron contamination in photon beams in the 20 MV range have been reported in recent years. In order to determine if the variations were due mainly to differences in measurement procedures, or inherent in the design of the accelerators, three different 18-MV (BJR) photon beams were compared using identical analytical techniques. The units studied were a Philips SL/75-20 and a Siemens Mevatron-20 linear accelerators and a Schimadzu betatron. Gamma spectroscopy of an activated aluminum foil was the method used. By comparing the relative amounts of neutron contamination, errors associated with absolute measurements such as detector efficiency and differences in activation foils were eliminated. Fast neutron contaminations per rad of x rays in a ratio of 6.7:3.7:1 were found for the Philips, Schimadzu and Siemens accelerators, respectively."}
{"id": "PMID:460068", "title": "Application of information theory to the assessment of computed tomography.", "content": "The imaging process has two fundamental stages: detection and display. The detection stage can be quantified rigourously using Shannon's information theory. This requires the contrast scale (CS), modulation transfer function (MTF), and noise power spectrum [N(f)] to be combined into a signal-to-noise ratio (SNR). This results in two fundamental summary figures of merit: the density of noise equivalent quanta (NEQ) in the image and the information bandwidth integral (IBWI). These algorithm-independent measures are used to quantify the recording stage. The display stage is less well understood since it couples to an external observer. Several types of decision makers are treated. Examples are drawn from first and second generation CT, demonstrating that thye are nearly quantum limited for large signals, indicating how their algorithms are matched or mismatched to the geometry, and calculating the contrast-detail diagrams for those decision makers.", "contents": "Application of information theory to the assessment of computed tomography. The imaging process has two fundamental stages: detection and display. The detection stage can be quantified rigourously using Shannon's information theory. This requires the contrast scale (CS), modulation transfer function (MTF), and noise power spectrum [N(f)] to be combined into a signal-to-noise ratio (SNR). This results in two fundamental summary figures of merit: the density of noise equivalent quanta (NEQ) in the image and the information bandwidth integral (IBWI). These algorithm-independent measures are used to quantify the recording stage. The display stage is less well understood since it couples to an external observer. Several types of decision makers are treated. Examples are drawn from first and second generation CT, demonstrating that thye are nearly quantum limited for large signals, indicating how their algorithms are matched or mismatched to the geometry, and calculating the contrast-detail diagrams for those decision makers."}
{"id": "PMID:460069", "title": "X-ray depth-dose characteristics of the Toshiba LMR-16.", "content": "The depth-dose characteristics of the Toshiba LMR-16 linear accelerator for 14-MeV x rays have been measured at an SSD of 100 cm using diodes and ion chambers. The surface dose and build-up depth both exhibit a considerable variation with field size. A new central axis model has been developed which takes account of these variations, and the agreement between the measured and computed data using this model is found to be excellent. Formulas are also presented to estimate the surface dose, buildup depth, and output factor as a function of field size.", "contents": "X-ray depth-dose characteristics of the Toshiba LMR-16. The depth-dose characteristics of the Toshiba LMR-16 linear accelerator for 14-MeV x rays have been measured at an SSD of 100 cm using diodes and ion chambers. The surface dose and build-up depth both exhibit a considerable variation with field size. A new central axis model has been developed which takes account of these variations, and the agreement between the measured and computed data using this model is found to be excellent. Formulas are also presented to estimate the surface dose, buildup depth, and output factor as a function of field size."}
{"id": "PMID:460172", "title": "Uptake of immune RNA by normal mouse spleen cells.", "content": "Normal mouse spleen cells take up in vitro radioactively labeled immune RNA. RNA taken up is present in nuclei, polysomes, membranes and cytoplasm. About 20-40% of immune RNA is nonspecifically associated with cell surface. 45% of RNA taken up is degraded and reutilized inside the cells within 2 hours.", "contents": "Uptake of immune RNA by normal mouse spleen cells. Normal mouse spleen cells take up in vitro radioactively labeled immune RNA. RNA taken up is present in nuclei, polysomes, membranes and cytoplasm. About 20-40% of immune RNA is nonspecifically associated with cell surface. 45% of RNA taken up is degraded and reutilized inside the cells within 2 hours."}
{"id": "PMID:460173", "title": "Purification and characterization of mouse glucose 6-phosphate dehydrogenase.", "content": "Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP- and -2', 5'-ADP-Sepharose columns and biospecific elution with NADP+ in both steps. The molecular and biochemical properties of the purified enzyme were studied in detail. These include the molecular weight determination, amino acid composition, steady-state kinetics, inactivation by high temperature, urea and iodoacetate, and immunology. The purified enzyme from mouse kidneys or testes was shown to be a tetramer with a molecular weight of 220,000. The enzyme is highly specific for glucose-6-phosphate, exhibits almost no activity with NAD+ as a coenzyme and is little inhibited by AMP or ATP. Michaelis constants for glucose-6-phosphate and NADP+ were determined to be 50 microM and 10 microM respectively. NADPH is a competitive inhibitor of NADP+ and has a Ki of 18 microM. Rabbit antisera against glucose-6-phosphate dehydrogenase were raised. The antisera also cross-react with the same enzyme from human and guinea pig.", "contents": "Purification and characterization of mouse glucose 6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP- and -2', 5'-ADP-Sepharose columns and biospecific elution with NADP+ in both steps. The molecular and biochemical properties of the purified enzyme were studied in detail. These include the molecular weight determination, amino acid composition, steady-state kinetics, inactivation by high temperature, urea and iodoacetate, and immunology. The purified enzyme from mouse kidneys or testes was shown to be a tetramer with a molecular weight of 220,000. The enzyme is highly specific for glucose-6-phosphate, exhibits almost no activity with NAD+ as a coenzyme and is little inhibited by AMP or ATP. Michaelis constants for glucose-6-phosphate and NADP+ were determined to be 50 microM and 10 microM respectively. NADPH is a competitive inhibitor of NADP+ and has a Ki of 18 microM. Rabbit antisera against glucose-6-phosphate dehydrogenase were raised. The antisera also cross-react with the same enzyme from human and guinea pig."}
{"id": "PMID:460175", "title": "Synthesis of phosphatidly-dCMP in permeabilized normal human lymphocytes.", "content": "When peripheral lymphocytes stimulated with phytohemagglutinin were permeabilized in vitro, (3H) dCTP acted as a precursor for DNA synthesis, but the formation of a compound soluble in organic solvents could also be demonstrated. The structure of the latter compound was studied analyzing the products formed after alkaline hydrolysis or an enzymatic treatment with nucleotide pyrophosphatase. Both treatments led to the formation of (3H)dCMP. When stimulated lymphocytes were labeled in vivo with (14C)glycerol before permeabilization and ulterior labeling with (3H)dCTP a double labeled compound was obtained. When this compound was submitted to alkaline hydrolysis, (3H)dCMP and (14C)glycerol-3-phosphate were obtained. It was concluded that the compound soluble in organic solvents was phosphatidyl-dCMP.", "contents": "Synthesis of phosphatidly-dCMP in permeabilized normal human lymphocytes. When peripheral lymphocytes stimulated with phytohemagglutinin were permeabilized in vitro, (3H) dCTP acted as a precursor for DNA synthesis, but the formation of a compound soluble in organic solvents could also be demonstrated. The structure of the latter compound was studied analyzing the products formed after alkaline hydrolysis or an enzymatic treatment with nucleotide pyrophosphatase. Both treatments led to the formation of (3H)dCMP. When stimulated lymphocytes were labeled in vivo with (14C)glycerol before permeabilization and ulterior labeling with (3H)dCTP a double labeled compound was obtained. When this compound was submitted to alkaline hydrolysis, (3H)dCMP and (14C)glycerol-3-phosphate were obtained. It was concluded that the compound soluble in organic solvents was phosphatidyl-dCMP."}
{"id": "PMID:460176", "title": "Polymorphism in fowl serum albumin. VI. Changes in in vitro protein synthesizing activity in developing embryonic fowl liver.", "content": "Cell-free protein synthesizing systems were prepared from the livers of chick embryos at selected ages and the characteristics of individual fractions were compared. While polysomes showed decreasing size with older embryos, isolated polysomes did not differ significantly in amino acid incorporating activity when assayed with standard cell sap. When assayed with standard polysomes, cell sap activity decreased with increasing developmental age whether incorporation was measured using (3H)lysine, (3H)leucine, or [3H]aminoacyl-tRNA. Free amino acid concentrations in the cell sap showed reproducidble independent variation during development which was taken into consideration in calculating net amino acid incorporation. A larger increase in ribonuclease activity was observed during development; however, nuclease inhibitor activity was absent before day 15 but increased thereafter. Aminoacyl-tRNA sythetase activity did not vary significantly. It is proposed that the observed changes in the rate of cell-free protein synthesis result not only from increasing ribonuclease activity with increasing developmental age but also from changes in the activity of other soluble factors.", "contents": "Polymorphism in fowl serum albumin. VI. Changes in in vitro protein synthesizing activity in developing embryonic fowl liver. Cell-free protein synthesizing systems were prepared from the livers of chick embryos at selected ages and the characteristics of individual fractions were compared. While polysomes showed decreasing size with older embryos, isolated polysomes did not differ significantly in amino acid incorporating activity when assayed with standard cell sap. When assayed with standard polysomes, cell sap activity decreased with increasing developmental age whether incorporation was measured using (3H)lysine, (3H)leucine, or [3H]aminoacyl-tRNA. Free amino acid concentrations in the cell sap showed reproducidble independent variation during development which was taken into consideration in calculating net amino acid incorporation. A larger increase in ribonuclease activity was observed during development; however, nuclease inhibitor activity was absent before day 15 but increased thereafter. Aminoacyl-tRNA sythetase activity did not vary significantly. It is proposed that the observed changes in the rate of cell-free protein synthesis result not only from increasing ribonuclease activity with increasing developmental age but also from changes in the activity of other soluble factors."}
{"id": "PMID:460177", "title": "Growth hormone effects on creatine uptake by muscle in the hypophysectomized rat.", "content": "Specific radioactive enzyme assays were developed to measure the effect of growth hormone on kidney transamidinase and liver methyltransferase in the hypophysectomized rat. In contrast to minimal changes (20%) in liver methyltransferase, kidney transamidinase was decreased threefold in the hypophysectomized rat. Enzyme activities were equal to normal values in those rats receiving growth hormone for three days. The formation of creatine from radioactive precursors and the uptake of 14C-creatine in muscle was examined under these conditions. After injection of 14C-arginine in the hypophysectomized rat, the 14C-creatine content of muscle was greatly decreased compared to sham operated controls and the 14C-creatine content was normal after growth hormone administration. After injection of 14C-guanidoacetate and of 14-creatine, the 14C-creatine content of muscle was decreased in the hypophysectomized rat, but was equal to sham control values in rats receiving growth hormone. These studies indicate that the uptake of newly synthesized creatine by muscle is impaired in the hypophysectomized rat and that growth hormone can have a role in controlling the rate of creatine uptake by muscle in addition to its effect on kidney transamidinase and to other factors involved in creatine metabolism.", "contents": "Growth hormone effects on creatine uptake by muscle in the hypophysectomized rat. Specific radioactive enzyme assays were developed to measure the effect of growth hormone on kidney transamidinase and liver methyltransferase in the hypophysectomized rat. In contrast to minimal changes (20%) in liver methyltransferase, kidney transamidinase was decreased threefold in the hypophysectomized rat. Enzyme activities were equal to normal values in those rats receiving growth hormone for three days. The formation of creatine from radioactive precursors and the uptake of 14C-creatine in muscle was examined under these conditions. After injection of 14C-arginine in the hypophysectomized rat, the 14C-creatine content of muscle was greatly decreased compared to sham operated controls and the 14C-creatine content was normal after growth hormone administration. After injection of 14C-guanidoacetate and of 14-creatine, the 14C-creatine content of muscle was decreased in the hypophysectomized rat, but was equal to sham control values in rats receiving growth hormone. These studies indicate that the uptake of newly synthesized creatine by muscle is impaired in the hypophysectomized rat and that growth hormone can have a role in controlling the rate of creatine uptake by muscle in addition to its effect on kidney transamidinase and to other factors involved in creatine metabolism."}
{"id": "PMID:460178", "title": "The size distribution of poly(A) in newly synthesized and old Balbiani ring RNA.", "content": "The size distribution of newly synthesized and old poly(A) sequences on transcripts of the giant tissue specific puffs, Balbiani rings in salivary glands of Chironomus tentans has been determined. After labeling with [3H]adenosine, poly(A) containing Balbiani ring RNA(75S RNA) was selectively collected by means of a recently developed technique. This combines electrophoretic fractionation and affinity chromatography in one run by insertion of poly(U) immobilized in glass fiber filters in an agarose gel slab. The majority of short-term labeled poly(A) chains released from poly(A) containing 75S RNA molecules is distributed within a narrow size range migrating as one peak with a mean value of 103 +/- 2 nucleotides, which is probably the initial length of poly(A). The labeling pattern of ribonuclease resistant poly(A) stretches after chase with unlabeled adenosine displays a relatively broad and heterogeneous size spectrum from at least 20 to more than 100 nucleotides. The main peak of labeled adenylate core in newly formed poly(A) containing RNA of non-Balbiani ring origin is dispersed within a broader size range than that of Balbiani ring RNA and possesses an average value of 94 +/- 2 nucleotides. During chase conditions, the relative frequency of occurrence of poly(A) chains of 75S RNA in the size range of 100 nucleotides exhibits a significant decrease in parallel with a rather uniform gain in the size classes between 20--50 nucleotides. However, the results are inconsistent with the existence of an age-dependent shortening of poly(A) chains in the balbiani ring RNA. A significant portion of 75S RNA molecules remain associated with poly(A) segments which are essentially of original size even after 21 hr in the presence of unlabeled adenosine. This finding provides support for the possibility that the initiation of the poly(A) shortening in 75S RNA is a stochastic process.", "contents": "The size distribution of poly(A) in newly synthesized and old Balbiani ring RNA. The size distribution of newly synthesized and old poly(A) sequences on transcripts of the giant tissue specific puffs, Balbiani rings in salivary glands of Chironomus tentans has been determined. After labeling with [3H]adenosine, poly(A) containing Balbiani ring RNA(75S RNA) was selectively collected by means of a recently developed technique. This combines electrophoretic fractionation and affinity chromatography in one run by insertion of poly(U) immobilized in glass fiber filters in an agarose gel slab. The majority of short-term labeled poly(A) chains released from poly(A) containing 75S RNA molecules is distributed within a narrow size range migrating as one peak with a mean value of 103 +/- 2 nucleotides, which is probably the initial length of poly(A). The labeling pattern of ribonuclease resistant poly(A) stretches after chase with unlabeled adenosine displays a relatively broad and heterogeneous size spectrum from at least 20 to more than 100 nucleotides. The main peak of labeled adenylate core in newly formed poly(A) containing RNA of non-Balbiani ring origin is dispersed within a broader size range than that of Balbiani ring RNA and possesses an average value of 94 +/- 2 nucleotides. During chase conditions, the relative frequency of occurrence of poly(A) chains of 75S RNA in the size range of 100 nucleotides exhibits a significant decrease in parallel with a rather uniform gain in the size classes between 20--50 nucleotides. However, the results are inconsistent with the existence of an age-dependent shortening of poly(A) chains in the balbiani ring RNA. A significant portion of 75S RNA molecules remain associated with poly(A) segments which are essentially of original size even after 21 hr in the presence of unlabeled adenosine. This finding provides support for the possibility that the initiation of the poly(A) shortening in 75S RNA is a stochastic process."}
{"id": "PMID:460180", "title": "Postribosomal complexes containing eukaryotic initiation factor eIF-2.", "content": "Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor.", "contents": "Postribosomal complexes containing eukaryotic initiation factor eIF-2. Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor."}
{"id": "PMID:460182", "title": "Free cytoplasmic messenger ribonucleoprotein complexes from rabbit reticulocytes.", "content": "Free cytoplasmic globin mRNA containing mRNP-particles were isolated from rabbit reticulocytes by zonal sucrose gradient centrifugation and their properties were compared with mRNP particles isolated in the same way from EDTA-dissociated reticulocyte polyribosomes. The average poly(A)-length of 9S mRNA from free cytoplasmic mRNP was 17-20 nucleotides being about two times shorter than the average poly(A)-length of polysomal 9S mRNA. The protein composition of the free cytoplasmic mRNP particles disclosed the absence of the 76,000 dalton protein which is associated with the 3'poly(A)-segment of polysomal globin mRNA. It was concluded that free cytoplasmic mRNP-particles from rabbit reticulocytes can be classified as \"old\" mRNP in a post-translational phase. Free cytoplasmic mRNPs were translated in heterologous cell-free systems as well as in Xenopus laevis oocytes. Addition of hemin stimulated the synthesis of alpha-globin in all systems, while the presence of the cap analogue m7G(5')p inhibited translation of free cytoplasmic mRNA completely. The latter finding suggested that free cytoplasmic mRNA has a 5' terminal \"cap\". Shortening of the poly(A)-segment with concomitant loss of the 76,000 dalton protein may lead to less efficient translation of free cytoplasmic mRNP.", "contents": "Free cytoplasmic messenger ribonucleoprotein complexes from rabbit reticulocytes. Free cytoplasmic globin mRNA containing mRNP-particles were isolated from rabbit reticulocytes by zonal sucrose gradient centrifugation and their properties were compared with mRNP particles isolated in the same way from EDTA-dissociated reticulocyte polyribosomes. The average poly(A)-length of 9S mRNA from free cytoplasmic mRNP was 17-20 nucleotides being about two times shorter than the average poly(A)-length of polysomal 9S mRNA. The protein composition of the free cytoplasmic mRNP particles disclosed the absence of the 76,000 dalton protein which is associated with the 3'poly(A)-segment of polysomal globin mRNA. It was concluded that free cytoplasmic mRNP-particles from rabbit reticulocytes can be classified as \"old\" mRNP in a post-translational phase. Free cytoplasmic mRNPs were translated in heterologous cell-free systems as well as in Xenopus laevis oocytes. Addition of hemin stimulated the synthesis of alpha-globin in all systems, while the presence of the cap analogue m7G(5')p inhibited translation of free cytoplasmic mRNA completely. The latter finding suggested that free cytoplasmic mRNA has a 5' terminal \"cap\". Shortening of the poly(A)-segment with concomitant loss of the 76,000 dalton protein may lead to less efficient translation of free cytoplasmic mRNP."}
{"id": "PMID:460183", "title": "Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina.", "content": "Conditions for the isolation of intact poly(A)+mRNP from cryptobiotic gastruale of A. salina are described. In the presence of Mg2+ ions nucleolytic cleavage occurs in vitro in the vicinity of the 3'-poly(A) segment of mRNP during the isolation procedure. The resulting two parts of poly(A)+mRNP complex are separated by thermal elution from oligo(dT)-cellulsoe affinity column. Analysis by SDS-gel electrophoresis of protein components associated with intact poly(A)+mRNP has revealed the existence of 20--30 S RNP complex containing five major proteins with Mr 68,000, 53,000, 50,000, 45,000 and 38,000, respectively, but completely lacking the poly(A)-specific Mr 76,000 protein.", "contents": "Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina. Conditions for the isolation of intact poly(A)+mRNP from cryptobiotic gastruale of A. salina are described. In the presence of Mg2+ ions nucleolytic cleavage occurs in vitro in the vicinity of the 3'-poly(A) segment of mRNP during the isolation procedure. The resulting two parts of poly(A)+mRNP complex are separated by thermal elution from oligo(dT)-cellulsoe affinity column. Analysis by SDS-gel electrophoresis of protein components associated with intact poly(A)+mRNP has revealed the existence of 20--30 S RNP complex containing five major proteins with Mr 68,000, 53,000, 50,000, 45,000 and 38,000, respectively, but completely lacking the poly(A)-specific Mr 76,000 protein."}
{"id": "PMID:460184", "title": "Regulation of albumin synthesis in rat liver.", "content": "The present report reviews our findings on the subcellular distribution of albumin mRNA in rat liver under normal and abnormal physiologic conditions, the identification of albumin mRNA in specific mRNP complexes in liver cytosol of starved rats, and evidence fo albumin mRNA sequences in a higher molecular weight nuclear precursor to cytoplasmic albumin mRNA.", "contents": "Regulation of albumin synthesis in rat liver. The present report reviews our findings on the subcellular distribution of albumin mRNA in rat liver under normal and abnormal physiologic conditions, the identification of albumin mRNA in specific mRNP complexes in liver cytosol of starved rats, and evidence fo albumin mRNA sequences in a higher molecular weight nuclear precursor to cytoplasmic albumin mRNA."}
{"id": "PMID:460186", "title": "Is the cytoskeleton-plasma membrane complex involved in lens protein biosynthesis?", "content": "Calf lens fiber cells contain a population of polyribosomes that direct, at least in vitro, the synthesis of a specific plasma membrane protein MP26. This protein may serve as a marker in terminal differentiation, since it is absent in the lens epithelium but appears in lens fiber plasma membranes. The MP26 manufacturing polyribosomes are found to be associated with a structural complex in which also the cytoskeleton and plasma membranes participate. They can be released from the complex by treatment with DNAse I. This result presumably reflects the involvement of actin in the linkage of the MP26 synthesizing polyribosomes to the cytoskeleton-membrane complex.", "contents": "Is the cytoskeleton-plasma membrane complex involved in lens protein biosynthesis? Calf lens fiber cells contain a population of polyribosomes that direct, at least in vitro, the synthesis of a specific plasma membrane protein MP26. This protein may serve as a marker in terminal differentiation, since it is absent in the lens epithelium but appears in lens fiber plasma membranes. The MP26 manufacturing polyribosomes are found to be associated with a structural complex in which also the cytoskeleton and plasma membranes participate. They can be released from the complex by treatment with DNAse I. This result presumably reflects the involvement of actin in the linkage of the MP26 synthesizing polyribosomes to the cytoskeleton-membrane complex."}
{"id": "PMID:460188", "title": "[Metabolically stable classes of nuclear messenger-like RNA. I. Detection and molecular characterization].", "content": "Two discreet in size molecular classes of metabolically stable messenger-like RNA molecules with sedimentation coefficientes about 28S and 18S have been revealed in the nuclei of pigeon bone marrow cells. The structural pecularities of 28S RNA class were investigated more carefully. It was shown to constitute the largest fraction of nuclear messenger-like RNA and is characterized by a GC/AU ratio 1.13. As pulse-labeled nuclear RNA, 28S stable nuclear RNA hybridizes with the unique and rare repeated DNA, it does not contain poly(A)-sequences and is found in a form of RNP particles with density of 1.41 g/sm3, tightly bound to chromatin.", "contents": "[Metabolically stable classes of nuclear messenger-like RNA. I. Detection and molecular characterization]. Two discreet in size molecular classes of metabolically stable messenger-like RNA molecules with sedimentation coefficientes about 28S and 18S have been revealed in the nuclei of pigeon bone marrow cells. The structural pecularities of 28S RNA class were investigated more carefully. It was shown to constitute the largest fraction of nuclear messenger-like RNA and is characterized by a GC/AU ratio 1.13. As pulse-labeled nuclear RNA, 28S stable nuclear RNA hybridizes with the unique and rare repeated DNA, it does not contain poly(A)-sequences and is found in a form of RNP particles with density of 1.41 g/sm3, tightly bound to chromatin."}
{"id": "PMID:460189", "title": "[Bis-(2-guanidoethyl)-disulfide, when complexed with DNA, prevents its winding within the B-family].", "content": "Earlier we have shown that DNA forms stoichiometric complexes with bis-(2-guanidoethyl)-disulfide (GED), the helix being transformed from the B-like structure into the C-like one. The present work demonstrates that the DNA helix in the saturated complex does not change its conformation within a broad range of conditions: 0 less than [NaCl] less than 5.10(-2) M; 0% less than [methanol] less than 60% (v/v); 5 degrees less than temperature less than 50 degrees. Free DNA undergoes a non-cooperative winding within the B-family under all these influences. Increase in NaCl concentration beyond 5.10(-2) M results in abrupt cooperative unwinding from the C-like form to the B-like one, apparently due to GED dissociation. The obtained data are in accord with the idea that the conformational transition of DNA within the B-family is induced by change of phosphates interaction with the hydrated cations of alcaline metals; formation of strong hydrogen bonds of the phosphates with GED makes the DNA helix non-sensitive to variations of the environment.", "contents": "[Bis-(2-guanidoethyl)-disulfide, when complexed with DNA, prevents its winding within the B-family]. Earlier we have shown that DNA forms stoichiometric complexes with bis-(2-guanidoethyl)-disulfide (GED), the helix being transformed from the B-like structure into the C-like one. The present work demonstrates that the DNA helix in the saturated complex does not change its conformation within a broad range of conditions: 0 less than [NaCl] less than 5.10(-2) M; 0% less than [methanol] less than 60% (v/v); 5 degrees less than temperature less than 50 degrees. Free DNA undergoes a non-cooperative winding within the B-family under all these influences. Increase in NaCl concentration beyond 5.10(-2) M results in abrupt cooperative unwinding from the C-like form to the B-like one, apparently due to GED dissociation. The obtained data are in accord with the idea that the conformational transition of DNA within the B-family is induced by change of phosphates interaction with the hydrated cations of alcaline metals; formation of strong hydrogen bonds of the phosphates with GED makes the DNA helix non-sensitive to variations of the environment."}
{"id": "PMID:460187", "title": "[Conformation mobility of the universal acceptor end of tRNA].", "content": "The conformation analysis of tRNA acceptor fragment XCCA (X=A) was performed. The ribose conformation was taken rigid C(3')-endo. The optimum conformation was found for the ACCA fragment. It was shown that the omega, omega'-angle of the terminal adenine in any crystal modification of the yeast tRNAFen is not optimal. The conformation mobility of the 3'-end ACCA fragment was calculated in the vicinity of optimum conformation. Redundant energy required for keeping the correct 3'-terminal tRNA orientation about the enzyme catalytic centre during any small orientation distortions of the non-universal X-nucleotide relative to the enzyme was estimated. The distortions may occur when non-gomologous tRNA interact with aminoacyl-tRNA-synthetase. It was found that redundant energy for 3' displacement is very anisotropic and some small X-nucleotide reorientation may decrease the catalitic velocity of aminoacylation u0(4)--10(5) times.", "contents": "[Conformation mobility of the universal acceptor end of tRNA]. The conformation analysis of tRNA acceptor fragment XCCA (X=A) was performed. The ribose conformation was taken rigid C(3')-endo. The optimum conformation was found for the ACCA fragment. It was shown that the omega, omega'-angle of the terminal adenine in any crystal modification of the yeast tRNAFen is not optimal. The conformation mobility of the 3'-end ACCA fragment was calculated in the vicinity of optimum conformation. Redundant energy required for keeping the correct 3'-terminal tRNA orientation about the enzyme catalytic centre during any small orientation distortions of the non-universal X-nucleotide relative to the enzyme was estimated. The distortions may occur when non-gomologous tRNA interact with aminoacyl-tRNA-synthetase. It was found that redundant energy for 3' displacement is very anisotropic and some small X-nucleotide reorientation may decrease the catalitic velocity of aminoacylation u0(4)--10(5) times."}
{"id": "PMID:460190", "title": "[Conformation of the DNA molecule in alcohol-water solvents].", "content": "The influence of different alcohol-water solvents on the conformation of the native DNA molecule has been studied by the methods of flow birefringence and viscometry. The experiments have shown, that a certain content of the organic component induces a cooperative transition in the tertiary structure of the DNA molecule. The conformational transitions in the tertiary structure of DNA have been observed at low alcohol concentrations, corresponding to the desruption of the ordered spatial water structure and are caused by the alteration of the intermolecular interactions between the DNA and solvent molecules. The alcohol concentrations, corresponding to the observed transition, as well, as relative decrease of the intrinsic viscosity of DNA, depend on the chain length and branching of the hydrocarbon portion of the alcohols. The data on the optical anisotropy of the DNA molecule testify that the secondary structure of the latter doesn't change in a wide range of the alcohol concentrations.", "contents": "[Conformation of the DNA molecule in alcohol-water solvents]. The influence of different alcohol-water solvents on the conformation of the native DNA molecule has been studied by the methods of flow birefringence and viscometry. The experiments have shown, that a certain content of the organic component induces a cooperative transition in the tertiary structure of the DNA molecule. The conformational transitions in the tertiary structure of DNA have been observed at low alcohol concentrations, corresponding to the desruption of the ordered spatial water structure and are caused by the alteration of the intermolecular interactions between the DNA and solvent molecules. The alcohol concentrations, corresponding to the observed transition, as well, as relative decrease of the intrinsic viscosity of DNA, depend on the chain length and branching of the hydrocarbon portion of the alcohols. The data on the optical anisotropy of the DNA molecule testify that the secondary structure of the latter doesn't change in a wide range of the alcohol concentrations."}
{"id": "PMID:460191", "title": "[Nuclear RNP, containing pre-mRNA. XIV. Nature of particles, containing 5'- and 3'-end structures].", "content": "Some characteristic peculiarities of the 5'-end and 3'-end structures of pre-mRNA isolated from nuclear RNP particles have been investigated: presence of triphosphorylated nucleotides on the 5'-ends, as a characteristic of the primary product of transcription; presence of modified 5'-ends -- blocked and methylated structures -- caps; presence of poly(A) blocks attached to the 3'-end of pre-mRNA during post-transcriptional transformation of the latter. It was shown that pre-mRNA isolated from nuclear RNP particles contained triphosphorylated nucleotides as well as a \"cap\" structure at the 5'-ends. On the 3'-ends of pre-mRNA from nuclear RNP particles isolated in the presence of a RNAse inhibitor, the presence of poly(A) blocks have been shown. These poly(A) structures are separated very easily from pre-mRNA during mild RNAase digestion. This is the reason why they are not detected in 30S monoparticles, containing pre-mRNA fragments connected with one informofer. Almost all poly(A) complexes in this condition are combined with proteins and have the sedimentation coefficient of about 14S. It was concluded that formation of nuclear pre-mRNA containing RNP-particles take place just after the onset of RNA synthesis. All processing steps occur with pre-mRNA packed in nuclear RNP particles.", "contents": "[Nuclear RNP, containing pre-mRNA. XIV. Nature of particles, containing 5'- and 3'-end structures]. Some characteristic peculiarities of the 5'-end and 3'-end structures of pre-mRNA isolated from nuclear RNP particles have been investigated: presence of triphosphorylated nucleotides on the 5'-ends, as a characteristic of the primary product of transcription; presence of modified 5'-ends -- blocked and methylated structures -- caps; presence of poly(A) blocks attached to the 3'-end of pre-mRNA during post-transcriptional transformation of the latter. It was shown that pre-mRNA isolated from nuclear RNP particles contained triphosphorylated nucleotides as well as a \"cap\" structure at the 5'-ends. On the 3'-ends of pre-mRNA from nuclear RNP particles isolated in the presence of a RNAse inhibitor, the presence of poly(A) blocks have been shown. These poly(A) structures are separated very easily from pre-mRNA during mild RNAase digestion. This is the reason why they are not detected in 30S monoparticles, containing pre-mRNA fragments connected with one informofer. Almost all poly(A) complexes in this condition are combined with proteins and have the sedimentation coefficient of about 14S. It was concluded that formation of nuclear pre-mRNA containing RNP-particles take place just after the onset of RNA synthesis. All processing steps occur with pre-mRNA packed in nuclear RNP particles."}
{"id": "PMID:460193", "title": "[Binding of muscle phosphorylase B by glycogen].", "content": "The binding of phosphorylase B with glycogen has been studied by means of analytical ultracentrifuge with absorption optics and a photoelectric scanner. Adsorbtion capacity for pig liver glycogen with respect to phosphorylase B (aM) and microscopic dissociation constant of enzyme -- glycogen complex (K) have been determined (aM = 3.64 . 10(-6) moles of bound enzyme per 1 g of glycogen: K = 2.6 . 10(-7) M at 12.7 degrees, pH 6.8). For oyster glycogen the value of aM is equal to 3.92 . 10(-6) moles of the bound protein per 1g of glycogen and K = 6.8 . 10(-7) M. The method of determination of microscopic Michaelis constant with respect to glycogen using known value of aM has been demonstrated.", "contents": "[Binding of muscle phosphorylase B by glycogen]. The binding of phosphorylase B with glycogen has been studied by means of analytical ultracentrifuge with absorption optics and a photoelectric scanner. Adsorbtion capacity for pig liver glycogen with respect to phosphorylase B (aM) and microscopic dissociation constant of enzyme -- glycogen complex (K) have been determined (aM = 3.64 . 10(-6) moles of bound enzyme per 1 g of glycogen: K = 2.6 . 10(-7) M at 12.7 degrees, pH 6.8). For oyster glycogen the value of aM is equal to 3.92 . 10(-6) moles of the bound protein per 1g of glycogen and K = 6.8 . 10(-7) M. The method of determination of microscopic Michaelis constant with respect to glycogen using known value of aM has been demonstrated."}
{"id": "PMID:460194", "title": "[Peculiarities of the reassociation kinetics of fast repeating sequences of thermolabile fractions of hepatoma Zaidel DNA].", "content": "The six fractions of rat liver DNA (DNAL) and hepatoma Zaidel DNA (DNAHZ) were obtained by thermal fractionation of these DNAs on a HAP column at equivalent temperature intervals. The percent of two thermolabile fractions obtained at 62--78 degrees is approximately 10% higher for DNAHZ than for DNAL. The reassociation kinetics were carried out till Cot = 5.10(-1). Computer analysis of the kinetics showed considerable difference between the parameters (percent of the sequence and rate constant) only for these thermolabile fractions DNAL and DNAHZ. The two-fold reduction of the reassociation rate for the second thermolabile fraction DNAHZ (63--78 degrees) can be associated with the presence of short uncomplementary pieces in the fast repeating sequences. On the other hand the increase of the rate constant for the initial part of the kinetics reassociation of the first thermolabile fraction (62--73 degrees) of DNAHZ is due to the nicks which are revealed by alkali sucrose centrifugation of the DNAHZ fractions.", "contents": "[Peculiarities of the reassociation kinetics of fast repeating sequences of thermolabile fractions of hepatoma Zaidel DNA]. The six fractions of rat liver DNA (DNAL) and hepatoma Zaidel DNA (DNAHZ) were obtained by thermal fractionation of these DNAs on a HAP column at equivalent temperature intervals. The percent of two thermolabile fractions obtained at 62--78 degrees is approximately 10% higher for DNAHZ than for DNAL. The reassociation kinetics were carried out till Cot = 5.10(-1). Computer analysis of the kinetics showed considerable difference between the parameters (percent of the sequence and rate constant) only for these thermolabile fractions DNAL and DNAHZ. The two-fold reduction of the reassociation rate for the second thermolabile fraction DNAHZ (63--78 degrees) can be associated with the presence of short uncomplementary pieces in the fast repeating sequences. On the other hand the increase of the rate constant for the initial part of the kinetics reassociation of the first thermolabile fraction (62--73 degrees) of DNAHZ is due to the nicks which are revealed by alkali sucrose centrifugation of the DNAHZ fractions."}
{"id": "PMID:460197", "title": "[Structure of nuclear pre-mRNA. XI. Triphosphorylated and blocked 5'-ends in the pre-mRNA].", "content": "The nature of the 5'-termini in pre-mRNA isolated from Ehrlich carcinoma cells has been investigated. To discriminate between triphosphorylated 5'-ends and capped structures different methods were used including treatment by alkaline phosphatase and several chromatographic methods. It was shown that heavey pre-mRNA contains a significant number of non-blocked triphosphorylated nucleotides at the 5'-end termini. However, phosphatase resistent, blocked 5'-termini were also found. 5'-terminal nucleotides in triphosphorylated pre-mRNA are G in a 3 : 2 ratio. In contrast to nuclear pre-mRNA cytoplasmic poly(A)+mRNA does not contain triphosphorylated 5'-ends but does contain the \"cap\" structure only. To elucidate the pre-mRNA topography the localization of homopolymeric regions of pre-mRNA, poly(A) and oligo(U), in relation to 5'terminal structures has been investigated. The experiments showed that the distance between 3'-terminal poly(A) sequences and 5'-end triphosphates is longer than 1500--2000 nucleotides. At the same time the distance between the latter and oligo(U) in pre-mRNA is much shorter.", "contents": "[Structure of nuclear pre-mRNA. XI. Triphosphorylated and blocked 5'-ends in the pre-mRNA]. The nature of the 5'-termini in pre-mRNA isolated from Ehrlich carcinoma cells has been investigated. To discriminate between triphosphorylated 5'-ends and capped structures different methods were used including treatment by alkaline phosphatase and several chromatographic methods. It was shown that heavey pre-mRNA contains a significant number of non-blocked triphosphorylated nucleotides at the 5'-end termini. However, phosphatase resistent, blocked 5'-termini were also found. 5'-terminal nucleotides in triphosphorylated pre-mRNA are G in a 3 : 2 ratio. In contrast to nuclear pre-mRNA cytoplasmic poly(A)+mRNA does not contain triphosphorylated 5'-ends but does contain the \"cap\" structure only. To elucidate the pre-mRNA topography the localization of homopolymeric regions of pre-mRNA, poly(A) and oligo(U), in relation to 5'terminal structures has been investigated. The experiments showed that the distance between 3'-terminal poly(A) sequences and 5'-end triphosphates is longer than 1500--2000 nucleotides. At the same time the distance between the latter and oligo(U) in pre-mRNA is much shorter."}
{"id": "PMID:460192", "title": "[Steady state kinetics of DNA degradation with pancreatic deoxyribonuclease A in the presence of Mg2+].", "content": "Steady state kinetics of DNA depolymerisation in the presence of the DNAase A and Mg2+ ions were investigated at pH 5.5 and wide region of the enzyme, substrate and metal ion concentrations. A model, which is consistent with experimental results obtained is suggested. According to the model catalytically active form of the DNAase A should be a metal-bound enzyme. That species reacts with the metal-free DNA to form the Michaelis complex. The kinetics observed can be described in terms of mechanism which involves covalent enzyme-substrate intermediate formation. It was shown that the second Mg2+ ion binding to the complex Mg2+ DNAase -- DNA (KD - 2.2 . 10(-3) M) enhances the kinetic parameters of the reaction. To rationalise the effect one has to assume that the rate of the intermediate formation was accelerated as a result of the second Mg2+ binding.", "contents": "[Steady state kinetics of DNA degradation with pancreatic deoxyribonuclease A in the presence of Mg2+]. Steady state kinetics of DNA depolymerisation in the presence of the DNAase A and Mg2+ ions were investigated at pH 5.5 and wide region of the enzyme, substrate and metal ion concentrations. A model, which is consistent with experimental results obtained is suggested. According to the model catalytically active form of the DNAase A should be a metal-bound enzyme. That species reacts with the metal-free DNA to form the Michaelis complex. The kinetics observed can be described in terms of mechanism which involves covalent enzyme-substrate intermediate formation. It was shown that the second Mg2+ ion binding to the complex Mg2+ DNAase -- DNA (KD - 2.2 . 10(-3) M) enhances the kinetic parameters of the reaction. To rationalise the effect one has to assume that the rate of the intermediate formation was accelerated as a result of the second Mg2+ binding."}
{"id": "PMID:460195", "title": "[RNA synthesis and modifications of heart nuclear proteins during thyroid hormone deficiency].", "content": "RNA synthesis, correlation of various histones and acetylation and phosphorylation of the chromatin proteins were studied in the rat heart during monthly hypothyroidism. It was shown that [3H]uridine incorporation into heart RNA decreases considerably at hypothyrosis. The alteration in relative amounts of the histone H4 subfractions, which does not depend on the method of hypothyrosis reproduction (inhibition of thyroid function by 1-methyl-2-mercaptoimidazole, thyroidectomy) was detected by the method of analytical electrophoresis in 15% polyacrylamide gels containing 3.125 M urea and 0.9 N acetic acid. Increased incorporation of [32P]phosphate into histone fraction H2b and total fraction of acidic chromatin proteins was observed in vivo. Increased incorporation of labeled acetate into the total histone fraction and reduced incorporation into acidic nuclear proteins were obtained. It was shown that the increased incorporation of acetate into the total histone fraction was due to the increased acetylation of histones H3, H2b, H4 and acid-soluble chromatin proteins characteristic of tissues with a low level of replication. It is assumed that the observed changes of nuclear proteins reflect the process of chromatin reorganization caused by a prolonged deficiency of thyroid hormones.", "contents": "[RNA synthesis and modifications of heart nuclear proteins during thyroid hormone deficiency]. RNA synthesis, correlation of various histones and acetylation and phosphorylation of the chromatin proteins were studied in the rat heart during monthly hypothyroidism. It was shown that [3H]uridine incorporation into heart RNA decreases considerably at hypothyrosis. The alteration in relative amounts of the histone H4 subfractions, which does not depend on the method of hypothyrosis reproduction (inhibition of thyroid function by 1-methyl-2-mercaptoimidazole, thyroidectomy) was detected by the method of analytical electrophoresis in 15% polyacrylamide gels containing 3.125 M urea and 0.9 N acetic acid. Increased incorporation of [32P]phosphate into histone fraction H2b and total fraction of acidic chromatin proteins was observed in vivo. Increased incorporation of labeled acetate into the total histone fraction and reduced incorporation into acidic nuclear proteins were obtained. It was shown that the increased incorporation of acetate into the total histone fraction was due to the increased acetylation of histones H3, H2b, H4 and acid-soluble chromatin proteins characteristic of tissues with a low level of replication. It is assumed that the observed changes of nuclear proteins reflect the process of chromatin reorganization caused by a prolonged deficiency of thyroid hormones."}
{"id": "PMID:460199", "title": "[Electronic-conformational interactions of molecular-biological systems. II. Study of the enzyme-substrate complex with the help of qualitative methods of quantum chemistry].", "content": "The electronic-conformational interactions (ECI) of enzyme-substrate complexes are treated with the help of the method of intermolecular orbitals. The applicability of this approach is shown concerning some problems, related to ECI. The activation of N2 in the active site of nitrogenase, the proton transfer in the system, containing hydrogen bonds, and the modelling of the initial state of the reaction of lysozyme with oligosaccharides were examined.", "contents": "[Electronic-conformational interactions of molecular-biological systems. II. Study of the enzyme-substrate complex with the help of qualitative methods of quantum chemistry]. The electronic-conformational interactions (ECI) of enzyme-substrate complexes are treated with the help of the method of intermolecular orbitals. The applicability of this approach is shown concerning some problems, related to ECI. The activation of N2 in the active site of nitrogenase, the proton transfer in the system, containing hydrogen bonds, and the modelling of the initial state of the reaction of lysozyme with oligosaccharides were examined."}
{"id": "PMID:460200", "title": "[Interrelation of the electronic structure of nucleic acid components and their ability and specificity in template synthesis].", "content": "A hypotesis suggesting that the specificity of polynucleotide template synthesis is based not on complementarity but on the correspondence of the electronic structure of the precursor and the enzyme active site (EAS), the latter being formed by the template, enzyme, and, possibly, by the polynucleotide synthesized is described. Comparison of the electronic structure of natural nucleic bases and their analogs allows to suppose that the EAS discriminates between adenine and cytosine, and uracil (thymine) and guanine, by electrostatic features: sign of the potential in the region of exocyclic substituents at C(4) of pyrimidines and C(5) of purines. For adenine and cytosine this sign is positive while for uracil (thymine) and guanine it is negative. The second feature allowing to discriminate between purines and pyrimidines is connected with general difference of their electronic structure. The total charge of N-glycoside center, more negative for pyrimidines, can serve as an index of this difference. According to the hypothesis the compounds unable to form complementary pairs can be functionally active in the polynucleotide template synthesis and can show ambiguous functional specificity due not only to the presence of different ionic and/or tautomeric species but also to the potential in the aforementioned region being close to zero or the charge of N-glycoside center being intermediate. It can be assumed that for the formation of EAS the same features of the electronic structure of the nucleotide residues of the template are used, which are important for the interaction of the precursor with the EAS (recognition of the precursor).", "contents": "[Interrelation of the electronic structure of nucleic acid components and their ability and specificity in template synthesis]. A hypotesis suggesting that the specificity of polynucleotide template synthesis is based not on complementarity but on the correspondence of the electronic structure of the precursor and the enzyme active site (EAS), the latter being formed by the template, enzyme, and, possibly, by the polynucleotide synthesized is described. Comparison of the electronic structure of natural nucleic bases and their analogs allows to suppose that the EAS discriminates between adenine and cytosine, and uracil (thymine) and guanine, by electrostatic features: sign of the potential in the region of exocyclic substituents at C(4) of pyrimidines and C(5) of purines. For adenine and cytosine this sign is positive while for uracil (thymine) and guanine it is negative. The second feature allowing to discriminate between purines and pyrimidines is connected with general difference of their electronic structure. The total charge of N-glycoside center, more negative for pyrimidines, can serve as an index of this difference. According to the hypothesis the compounds unable to form complementary pairs can be functionally active in the polynucleotide template synthesis and can show ambiguous functional specificity due not only to the presence of different ionic and/or tautomeric species but also to the potential in the aforementioned region being close to zero or the charge of N-glycoside center being intermediate. It can be assumed that for the formation of EAS the same features of the electronic structure of the nucleotide residues of the template are used, which are important for the interaction of the precursor with the EAS (recognition of the precursor)."}
{"id": "PMID:460196", "title": "[Single copy and repetitive nucleotide sequences in the genome of Echinodermata. II. Nucleotide sequence divergence of DNA of echinoderms].", "content": "The degree of divergence of short and long repetitive DNA sequences and single copy DNA of five Echinodermata species (sea urchins, starfish, sea-cucumber) was studied by the method of molecular hybridization. Different fractions of 3H-DNA of the sea urchin Strongylocentrotus intermedius were hybridized with the DNA of other species. Thermal stability of the hybridized DNA molecules was determined. The results obtained suggest that short repetitive sequences were most conservative during the evolution of Echinodermata. Single copy DNA fractions of closely related sea urchin species (S. intermedius and S. nudus) have more homologous sequences than long repetitive DNA fractions of the same species. The DNA of evolutionary distant species (sea urchin and starfish) have more homologous long repetitive sequences than the single copy ones. All DNA fractions of S. intermedius have sequences hybridized with the DNA of all other species studied: short repetitive sequences--55%, long repetitive sequences--20%, single copy sequences--12%.", "contents": "[Single copy and repetitive nucleotide sequences in the genome of Echinodermata. II. Nucleotide sequence divergence of DNA of echinoderms]. The degree of divergence of short and long repetitive DNA sequences and single copy DNA of five Echinodermata species (sea urchins, starfish, sea-cucumber) was studied by the method of molecular hybridization. Different fractions of 3H-DNA of the sea urchin Strongylocentrotus intermedius were hybridized with the DNA of other species. Thermal stability of the hybridized DNA molecules was determined. The results obtained suggest that short repetitive sequences were most conservative during the evolution of Echinodermata. Single copy DNA fractions of closely related sea urchin species (S. intermedius and S. nudus) have more homologous sequences than long repetitive DNA fractions of the same species. The DNA of evolutionary distant species (sea urchin and starfish) have more homologous long repetitive sequences than the single copy ones. All DNA fractions of S. intermedius have sequences hybridized with the DNA of all other species studied: short repetitive sequences--55%, long repetitive sequences--20%, single copy sequences--12%."}
{"id": "PMID:460203", "title": "[Globule size and the activation energy of an enzymatic process].", "content": "The charge transfer reactions demand the polar medium reorganization the main part in the process energy being contributed by solvent reorganization. Protein globule excludes a part of the solvent from the interaction with the charge being transfered. Thus a strong decrease of the reorganization energy and hence of the activation energy is achieved (the gain of some kcal/mole). The effect rises at first rapidly with the globule radius but it becomes practically constant after some optimal radius is reached. The estimation of the optimal radius gives values of the order of magnitude of the enzymes molecule sizes.", "contents": "[Globule size and the activation energy of an enzymatic process]. The charge transfer reactions demand the polar medium reorganization the main part in the process energy being contributed by solvent reorganization. Protein globule excludes a part of the solvent from the interaction with the charge being transfered. Thus a strong decrease of the reorganization energy and hence of the activation energy is achieved (the gain of some kcal/mole). The effect rises at first rapidly with the globule radius but it becomes practically constant after some optimal radius is reached. The estimation of the optimal radius gives values of the order of magnitude of the enzymes molecule sizes."}
{"id": "PMID:460202", "title": "[Effect of temperature and degree of hydration on the mobility of spin labels in surface layers of proteins].", "content": "The mobility of separate sites of the water-protein matrix depending on temperature and degree of hydration has been investigated by means of spin labels covalently attached to surface layers of proteins (alpha-chymotrypsin and human serum albumin) and also by a spin probe in a hydrophobic \"pocket\" of human serum albumin. The results obtained are compared with the data on the mobility of gamma-resonance labels (57Fe) firmly bound with the protein matrix in the same samples. At certain temperature and degree of hydration both spin and gamma-resonance label show an increase in mobility. With the degree of hydration increasing one may observe a simultaneous increase in energy and in entropy of activation: rotatory diffusion of spin labels, i. e., a compensation effect takes place which confirms the concept expressed earlier that cooperation of water-protein interactions is the main reason of CEF. It should be noted that at P/PS greater than 0.8 the values of delta E =7 divided by 10 kcal/mole, and delta S not equal to = 9 divided by 11 e. e. are specific to glycerol-like systems, i. e., under these conditions (P/Ps greater than 0.8) the water-protein layer has glycerol-like properties.", "contents": "[Effect of temperature and degree of hydration on the mobility of spin labels in surface layers of proteins]. The mobility of separate sites of the water-protein matrix depending on temperature and degree of hydration has been investigated by means of spin labels covalently attached to surface layers of proteins (alpha-chymotrypsin and human serum albumin) and also by a spin probe in a hydrophobic \"pocket\" of human serum albumin. The results obtained are compared with the data on the mobility of gamma-resonance labels (57Fe) firmly bound with the protein matrix in the same samples. At certain temperature and degree of hydration both spin and gamma-resonance label show an increase in mobility. With the degree of hydration increasing one may observe a simultaneous increase in energy and in entropy of activation: rotatory diffusion of spin labels, i. e., a compensation effect takes place which confirms the concept expressed earlier that cooperation of water-protein interactions is the main reason of CEF. It should be noted that at P/PS greater than 0.8 the values of delta E =7 divided by 10 kcal/mole, and delta S not equal to = 9 divided by 11 e. e. are specific to glycerol-like systems, i. e., under these conditions (P/Ps greater than 0.8) the water-protein layer has glycerol-like properties."}
{"id": "PMID:460205", "title": "[Synthesis and properties of nucleotide derivatives of poly(oligo)-L-lysine].", "content": "Nucleotide derivatives (epsilon-amino groups of lysine residues) of lysine residues) of poly(oligo)-L-lysine were synthesized. The structure of the resulting compounds in solution were studied by circular dichroism techniques. Week stacking interactions of the nucleotide bases on the polypeptide template were found to be characteristic of these compounds. It has been shown that the nucleotide derivatives of poly(oligo)-L-lysine are not involved in complementary interactions with the polynucleotides.", "contents": "[Synthesis and properties of nucleotide derivatives of poly(oligo)-L-lysine]. Nucleotide derivatives (epsilon-amino groups of lysine residues) of lysine residues) of poly(oligo)-L-lysine were synthesized. The structure of the resulting compounds in solution were studied by circular dichroism techniques. Week stacking interactions of the nucleotide bases on the polypeptide template were found to be characteristic of these compounds. It has been shown that the nucleotide derivatives of poly(oligo)-L-lysine are not involved in complementary interactions with the polynucleotides."}
{"id": "PMID:460201", "title": "[Regulation of enzyme activity in adsorptive enzyme systems. II. Influence of dextran sulfate on catalytic properties of lactate dehydrogenase (isozyme M4)].", "content": "It has been shown that the binding of pig skeletal muscle lactate dehydrogenase (isozyme M4) by dextran sulfate with weight-average molecular weight 500 000 is accompanied by a decrease of the rate of enzymatic reduction of pyruvate. The hyperbolic dependence of the enzymic reaction rate on NADH concentration observed for free lactate dehydrogenase is transformed in a sigmoidal curve in the case of adsorbed enzyme form (Hill's coefficient is equal to 2.1). The experimental data have been described quantitatively using the model of adsorptive enzyme system where the enzyme interacts reversibly with the support and co-operative interaction of substrate binding sites in the adsorbed enzyme molecule are realized. It is assumed that the value of microscopic dissociation constant for the complex of the substrate with adsorbed enzyme is being changed by a constant factor during saturation of the binding sites by the substrate in the enzyme molecule. The value of parameters of the model for the adsorptive enzyme system under study are determined.", "contents": "[Regulation of enzyme activity in adsorptive enzyme systems. II. Influence of dextran sulfate on catalytic properties of lactate dehydrogenase (isozyme M4)]. It has been shown that the binding of pig skeletal muscle lactate dehydrogenase (isozyme M4) by dextran sulfate with weight-average molecular weight 500 000 is accompanied by a decrease of the rate of enzymatic reduction of pyruvate. The hyperbolic dependence of the enzymic reaction rate on NADH concentration observed for free lactate dehydrogenase is transformed in a sigmoidal curve in the case of adsorbed enzyme form (Hill's coefficient is equal to 2.1). The experimental data have been described quantitatively using the model of adsorptive enzyme system where the enzyme interacts reversibly with the support and co-operative interaction of substrate binding sites in the adsorbed enzyme molecule are realized. It is assumed that the value of microscopic dissociation constant for the complex of the substrate with adsorbed enzyme is being changed by a constant factor during saturation of the binding sites by the substrate in the enzyme molecule. The value of parameters of the model for the adsorptive enzyme system under study are determined."}
{"id": "PMID:460210", "title": "[Interfragmental interaction in peptide molecules].", "content": "Orbital and Coulomb terms in total energy of the molecule may be estimated with the use of parameters, that were calculated from experimental or estimated data for the test molecules. It has been demonstrated how the parameters for calculating the barriers of internal rotation in ethane-like molecules and amides may be selected. These parameters were used for studying the interactions between two peptide groups in 2-formamideacetamide--the primitive model of peptide.", "contents": "[Interfragmental interaction in peptide molecules]. Orbital and Coulomb terms in total energy of the molecule may be estimated with the use of parameters, that were calculated from experimental or estimated data for the test molecules. It has been demonstrated how the parameters for calculating the barriers of internal rotation in ethane-like molecules and amides may be selected. These parameters were used for studying the interactions between two peptide groups in 2-formamideacetamide--the primitive model of peptide."}
{"id": "PMID:460206", "title": "[mRNA sequences complementary to the double-stranded regions of pre-mRNA. Hybridization experiments using RNA mercuriation and chromatography on sulfhydryl-Sepharose].", "content": "It was demonstrated that 4--6% of the sequences of mouse cell pure polysomal poly (A)mRNA are able to form stable duplexes during hybridization with the excess of double-stranded structures of nuclear pre-mRNA (double-stranded RNA). Hybridization of mRNA with the excess of mercuriated double-stranded RNA and chromatography of the hybrids on sulfhydril-sepharose was investigated. It was found that only 7--11% of the molecules of polysomal poly(A)+mRNA are able to form thermostable hybrids with sequences of double-stranded RNA. It may be supposed that in the population of mRNA there are relatively few molecules (about 5%) which contain sequences originated from long hairpin structures of pre-mRNA. Moreover, probably, there is a number of mRNA molecules (about 5% of the mRNA population) which are complementary in the entire length to \"non-hairpin\" double-stranded regions of pre-mRNA.", "contents": "[mRNA sequences complementary to the double-stranded regions of pre-mRNA. Hybridization experiments using RNA mercuriation and chromatography on sulfhydryl-Sepharose]. It was demonstrated that 4--6% of the sequences of mouse cell pure polysomal poly (A)mRNA are able to form stable duplexes during hybridization with the excess of double-stranded structures of nuclear pre-mRNA (double-stranded RNA). Hybridization of mRNA with the excess of mercuriated double-stranded RNA and chromatography of the hybrids on sulfhydril-sepharose was investigated. It was found that only 7--11% of the molecules of polysomal poly(A)+mRNA are able to form thermostable hybrids with sequences of double-stranded RNA. It may be supposed that in the population of mRNA there are relatively few molecules (about 5%) which contain sequences originated from long hairpin structures of pre-mRNA. Moreover, probably, there is a number of mRNA molecules (about 5% of the mRNA population) which are complementary in the entire length to \"non-hairpin\" double-stranded regions of pre-mRNA."}
{"id": "PMID:460204", "title": "[Molecular mechanism of self-assembly of aggregated bacteriochlorophyll c].", "content": "The intermolecular interaction of bacteriochlorophyll c and its pheophytin was studied in nonpolar solvents and solid films with the aid of absorption and infra-red (in the region of 1800--1600 and 3800--3000 cm-1) spectra. The influence of water removing and its addition on these spectra has been investigated. Besides the effect of pyridine treatment and pigment concentration were examined. The self-assemblage of all types of bacteriochlorophyll c aggregated forms absorbing in the range 680--745 nm is due to the formation of intermolecular bonds in which keto groups of cyclopentanone rings take part. Keto groups form coordinate bonds with the central magnesium atom (keto-C = O...Mg). Hydroxyl groups interact coordinately with magnesium and simultaneously form hydrogen bonds with pyrrol nitrogen. In contrast to chlorophyll a and bacteriochlorophyll a, water molecules in the case of bacteriochlorophyll c do not participate in the intermolecular bond formation in the course of long-wave aggregated forms production. The thermostability of bacteriochlorophyll c aggregates and their rather high stability to desaggregating agents is related to the mentioned peculiarities of their structure. Bacteriopheophytin c in any state (solution or solid film) is not capable to form intermolecular bonds by its carbonyl groups and long-wave aggregates. The specific features of the assemblage of bacteriochlorophyll c aggregates modelling antenna of the green photosynthetic bacteria are discussed.", "contents": "[Molecular mechanism of self-assembly of aggregated bacteriochlorophyll c]. The intermolecular interaction of bacteriochlorophyll c and its pheophytin was studied in nonpolar solvents and solid films with the aid of absorption and infra-red (in the region of 1800--1600 and 3800--3000 cm-1) spectra. The influence of water removing and its addition on these spectra has been investigated. Besides the effect of pyridine treatment and pigment concentration were examined. The self-assemblage of all types of bacteriochlorophyll c aggregated forms absorbing in the range 680--745 nm is due to the formation of intermolecular bonds in which keto groups of cyclopentanone rings take part. Keto groups form coordinate bonds with the central magnesium atom (keto-C = O...Mg). Hydroxyl groups interact coordinately with magnesium and simultaneously form hydrogen bonds with pyrrol nitrogen. In contrast to chlorophyll a and bacteriochlorophyll a, water molecules in the case of bacteriochlorophyll c do not participate in the intermolecular bond formation in the course of long-wave aggregated forms production. The thermostability of bacteriochlorophyll c aggregates and their rather high stability to desaggregating agents is related to the mentioned peculiarities of their structure. Bacteriopheophytin c in any state (solution or solid film) is not capable to form intermolecular bonds by its carbonyl groups and long-wave aggregates. The specific features of the assemblage of bacteriochlorophyll c aggregates modelling antenna of the green photosynthetic bacteria are discussed."}
{"id": "PMID:460211", "title": "[Theoretical analysis of action of substrate analogs in dissociating enzyme systems].", "content": "The action of substrate analogue inable to catalytic conversion has been analysed in dissociating enzyme system of the type 2p in equilibrium with P (P is enzyme oligomer and p is its half). The regions of parameters corresponding to dependences of enzyme reaction rate (v) on substrate analogue (H) concentration with maximum are calculated. It is characteristic of dissociating enzyme systems that the appearance of maximum depends on enzyme concentration. It has also been demonstrated that at definite values of parameters and values of enzyme and substrate concentration the v versus [H]o plots may show an intermediate plateau, maximum and its preceding S-shape or maximum and minimum simultaneously.", "contents": "[Theoretical analysis of action of substrate analogs in dissociating enzyme systems]. The action of substrate analogue inable to catalytic conversion has been analysed in dissociating enzyme system of the type 2p in equilibrium with P (P is enzyme oligomer and p is its half). The regions of parameters corresponding to dependences of enzyme reaction rate (v) on substrate analogue (H) concentration with maximum are calculated. It is characteristic of dissociating enzyme systems that the appearance of maximum depends on enzyme concentration. It has also been demonstrated that at definite values of parameters and values of enzyme and substrate concentration the v versus [H]o plots may show an intermediate plateau, maximum and its preceding S-shape or maximum and minimum simultaneously."}
{"id": "PMID:460209", "title": "[Selective modification of T7 DNA at the region of early genes by early RNA carrying multiple alkylating groups].", "content": "A method of selective modification of certain regions of the genome which may become useful for inactivation of certain genes or for directed mutagenesis is proposed. For this purpose RNA products of certain genes carrying alkylating groupings randomly distributed along the polymer were used. The RNA modified to an extent of 4--5 alkylating residues per 100 nucleotides retains the ability to specific formation of DNA--RNA hybrid complexes. The alkylating molecule is N,N,N'-tri-(beta-chlorethyl), N'-(p-formylphenyl)propylene diamine-1,3. The aliphatic alkylating functions serve for attachment to RNA. The aromatic alkylating function inactivated by the formyl grouping at the para-position of the benzene ring is used for modification of DNA after hybrid formation by reduction of formyl grouping with sodium borohydride. The covalently binding of modified RNA is exhibited to occur in only the case of T7 DNA H-chain, the one complementary to the RNA derivative. L-chain does not hybridize, nor does it undergo alkylation by the RNA product thus indicating high selectivity of alkylation within the hybrid complex.", "contents": "[Selective modification of T7 DNA at the region of early genes by early RNA carrying multiple alkylating groups]. A method of selective modification of certain regions of the genome which may become useful for inactivation of certain genes or for directed mutagenesis is proposed. For this purpose RNA products of certain genes carrying alkylating groupings randomly distributed along the polymer were used. The RNA modified to an extent of 4--5 alkylating residues per 100 nucleotides retains the ability to specific formation of DNA--RNA hybrid complexes. The alkylating molecule is N,N,N'-tri-(beta-chlorethyl), N'-(p-formylphenyl)propylene diamine-1,3. The aliphatic alkylating functions serve for attachment to RNA. The aromatic alkylating function inactivated by the formyl grouping at the para-position of the benzene ring is used for modification of DNA after hybrid formation by reduction of formyl grouping with sodium borohydride. The covalently binding of modified RNA is exhibited to occur in only the case of T7 DNA H-chain, the one complementary to the RNA derivative. L-chain does not hybridize, nor does it undergo alkylation by the RNA product thus indicating high selectivity of alkylation within the hybrid complex."}
{"id": "PMID:460207", "title": "[Secondary structure of RNA of influenza virus in free form and in ribonucleoprotein].", "content": "Large amounts of RNA and RNP isolated from influenza virus were obtained. This has allowed us to undertake detailed physical studies of the secondary structure of RNA of influenza virus in free form and in RNP. Analysis of CD spectrum and the hypochromic effect after thermal denaturation of RNA indicated that RNA in free form contains 58--62% double-stranded regions. By comparative studies of the secondary structure of RNA in RNP, it was estimated that 12--14% of the RNA exists in double-stranded form.", "contents": "[Secondary structure of RNA of influenza virus in free form and in ribonucleoprotein]. Large amounts of RNA and RNP isolated from influenza virus were obtained. This has allowed us to undertake detailed physical studies of the secondary structure of RNA of influenza virus in free form and in RNP. Analysis of CD spectrum and the hypochromic effect after thermal denaturation of RNA indicated that RNA in free form contains 58--62% double-stranded regions. By comparative studies of the secondary structure of RNA in RNP, it was estimated that 12--14% of the RNA exists in double-stranded form."}
{"id": "PMID:460208", "title": "[Mobility of collagen structure and temperature adaptation of animals].", "content": "The method of hydrogen exchange is used to determine the mobility of acid-soluble collagen molecules from animals with different physiological temperature in terms of equilibrium constants of the formation of micro-unfolding or fluctuating defects of the structure. It has been shown that mobility of the collagen structure correlates with the physiological temperature of the animal from whose tissue collagen was isolated and is mainly determined by the amino acid content of the collagen. It has been shown also that at physiological temperatures characteristic for a particular species the level of collagen mobility is of the same order despite their different thermostability. The conclusion has been drawn that the level of mobility is the main criterion at the natural selection of the amino acid composition of collagens in different animals.", "contents": "[Mobility of collagen structure and temperature adaptation of animals]. The method of hydrogen exchange is used to determine the mobility of acid-soluble collagen molecules from animals with different physiological temperature in terms of equilibrium constants of the formation of micro-unfolding or fluctuating defects of the structure. It has been shown that mobility of the collagen structure correlates with the physiological temperature of the animal from whose tissue collagen was isolated and is mainly determined by the amino acid content of the collagen. It has been shown also that at physiological temperatures characteristic for a particular species the level of collagen mobility is of the same order despite their different thermostability. The conclusion has been drawn that the level of mobility is the main criterion at the natural selection of the amino acid composition of collagens in different animals."}
{"id": "PMID:460213", "title": "[Role of surface distribution of hydrophobic groups in the assembly of protein quaternary structure].", "content": "The method for two-dimensional representation of protein surfaces, based on the analogy with geographic map, and the algorithm for the search of the most favourable regions of izologous intermolecular interactions are proposed. It is assumed that the most favourable izologous interaction corresponds to an izologous contact of subunits which maximally dehydrates surface hydrophobic groups. The subunits are approximated by ellipsoids of revolution. The hydrophobic groups are assumed to be dehydrated if their C beta-atoms are inside the surface regions of the contacting ellipsoids inaccessible for contact with the sphere representing a water molecule. Using the proposed methods it is shown that for all izologous structures, known to atomic resolution, general shape of the molecule and distribution of hydrophobic groups on its surface: 1) provide a rough intersubunit recognition during assembly (deviation of the center of the experimentally localized contact region from the center of one of two-three regions obtained by us does not exceed 6--7 A); 2) choose only a few most favourable mutual orientations of the contacting regions; 3) can determine the pathway of the assembly.", "contents": "[Role of surface distribution of hydrophobic groups in the assembly of protein quaternary structure]. The method for two-dimensional representation of protein surfaces, based on the analogy with geographic map, and the algorithm for the search of the most favourable regions of izologous intermolecular interactions are proposed. It is assumed that the most favourable izologous interaction corresponds to an izologous contact of subunits which maximally dehydrates surface hydrophobic groups. The subunits are approximated by ellipsoids of revolution. The hydrophobic groups are assumed to be dehydrated if their C beta-atoms are inside the surface regions of the contacting ellipsoids inaccessible for contact with the sphere representing a water molecule. Using the proposed methods it is shown that for all izologous structures, known to atomic resolution, general shape of the molecule and distribution of hydrophobic groups on its surface: 1) provide a rough intersubunit recognition during assembly (deviation of the center of the experimentally localized contact region from the center of one of two-three regions obtained by us does not exceed 6--7 A); 2) choose only a few most favourable mutual orientations of the contacting regions; 3) can determine the pathway of the assembly."}
{"id": "PMID:460256", "title": "[Treatment with immunocompetent cells and cellextracts (author's transl)].", "content": "The therapy of inborn or acquired defects of the cellular immune function is still in an experimental stage. Basicly, it is possible to replace the non-functioning T-cells by immunocompetent lymphocytes. Bone marrow, fetal liver and/or thymus, or cultivated thymus can serve as the source of such cells. Under certain conditions humoral factors derived from immunocompetent lymphocytes can induce a lacking immune response. Possibilities as well as limitations of these two therapeutic concepts are discussed.", "contents": "[Treatment with immunocompetent cells and cellextracts (author's transl)]. The therapy of inborn or acquired defects of the cellular immune function is still in an experimental stage. Basicly, it is possible to replace the non-functioning T-cells by immunocompetent lymphocytes. Bone marrow, fetal liver and/or thymus, or cultivated thymus can serve as the source of such cells. Under certain conditions humoral factors derived from immunocompetent lymphocytes can induce a lacking immune response. Possibilities as well as limitations of these two therapeutic concepts are discussed."}
{"id": "PMID:460257", "title": "[Cystometric investigations of neurogenic bladder-dysfunction in children (author's transl)].", "content": "Clinical and cystometrical studies were performed in 48 patients with myelomeningocele. From the clinical point of view four of the patients were completely continent. Fiften patients showed a so called \"social continence\" with periods of continence lasting for more than two hours (40%). Cystomanometric evaluations, however, revealed that there was a complete continence only in one of the 48 patients. Twenty-one patients showed a spastic or reflex bladder (44%), twentysix (54%) an adynamic bladder. The so called \"social continence\" could be observed as well in spastic-as in adynamic bladders. The most important parameters to realize neurogenic disturbances of micturition is the dyssynergia of the contraction of the detrusor muscle and the relaxation of the external vesical sphincter. Considering this, Stark's classification of adynamic and spastic bladders according to amplitude of the contractions of the detrusor muscle is helpful only in combination with the cystometrical and myographical datas of the bladder outlet.", "contents": "[Cystometric investigations of neurogenic bladder-dysfunction in children (author's transl)]. Clinical and cystometrical studies were performed in 48 patients with myelomeningocele. From the clinical point of view four of the patients were completely continent. Fiften patients showed a so called \"social continence\" with periods of continence lasting for more than two hours (40%). Cystomanometric evaluations, however, revealed that there was a complete continence only in one of the 48 patients. Twenty-one patients showed a spastic or reflex bladder (44%), twentysix (54%) an adynamic bladder. The so called \"social continence\" could be observed as well in spastic-as in adynamic bladders. The most important parameters to realize neurogenic disturbances of micturition is the dyssynergia of the contraction of the detrusor muscle and the relaxation of the external vesical sphincter. Considering this, Stark's classification of adynamic and spastic bladders according to amplitude of the contractions of the detrusor muscle is helpful only in combination with the cystometrical and myographical datas of the bladder outlet."}
{"id": "PMID:460260", "title": "[Quantitative determination of amino acids from blood-stained filter papers using high-performance thin layer chromatography and a TLC-scanner (author's transl)].", "content": "A method is described to determine amino acids by extraction from pieces of bloodstained and dried filter papers and following dansylation and separation by High-Performance Thin Layer Chromatography (HPTLC). Quantitative determination is possible by means of a TLC-Scanner measuring the fluorescent light of the dansylated amino acids. The possible use of this procedure as screening test is discussed.", "contents": "[Quantitative determination of amino acids from blood-stained filter papers using high-performance thin layer chromatography and a TLC-scanner (author's transl)]. A method is described to determine amino acids by extraction from pieces of bloodstained and dried filter papers and following dansylation and separation by High-Performance Thin Layer Chromatography (HPTLC). Quantitative determination is possible by means of a TLC-Scanner measuring the fluorescent light of the dansylated amino acids. The possible use of this procedure as screening test is discussed."}
{"id": "PMID:460261", "title": "[Salmonella-meningitis in the newborn (author's transl)].", "content": "Six children, out of twelve in a neonatal unit suffered from group D salmonellosis. Two patients presented in addition to intestinal manifestations massive extraintestinal symptoms, both with septicemia and meningitis. One patient died on the fourth day from massive disseminated intravascular coagulation and pyocephalus. The other patient had a complete recovery after an antibiotic therapy with chloramphenicol and ampicillin. As the source of infection the mother of case 1 was identified. In her stools salmonella group D were cultured. Cultures of the ward-personals, stool and the food were negative. It should be mentioned that only children fed with artificial food suffered from salmonellosis; whereas children on breastmilk had an unremarkable clinical course and consistantly negative stoolcultures.", "contents": "[Salmonella-meningitis in the newborn (author's transl)]. Six children, out of twelve in a neonatal unit suffered from group D salmonellosis. Two patients presented in addition to intestinal manifestations massive extraintestinal symptoms, both with septicemia and meningitis. One patient died on the fourth day from massive disseminated intravascular coagulation and pyocephalus. The other patient had a complete recovery after an antibiotic therapy with chloramphenicol and ampicillin. As the source of infection the mother of case 1 was identified. In her stools salmonella group D were cultured. Cultures of the ward-personals, stool and the food were negative. It should be mentioned that only children fed with artificial food suffered from salmonellosis; whereas children on breastmilk had an unremarkable clinical course and consistantly negative stoolcultures."}
{"id": "PMID:460287", "title": "Effect of cell growth rate and dose fractionation on chemically-induced  ouabain-resistant mutations in Chinese hamster V79 cells.", "content": "Chinese hamster V79 cells were grown in medium containing either 10% or 2% FCS during the expression time following exposure to MNNG. The lower serum concentration was used to reduce the rate of cell replication, thereby allowing more time for DNA repair prior to \"fixation\" of the mutagenic lesion. In addition, fractionated and continuous exposures to MNNG and MAM, respectively, were carried out to determine their effect on the number of induced ouabain-resistant mutants. The results indicated that lowering the rate of cell growth effectively reduces the mutation frequency at low, but not at high doses of MNNG. Fractionated doses of MNNG result in a potentiation of their mutagenic effects compared to single doses. Also, continuous exposures to MAM result in an exponential increase in the mutation frequency. Collectively, these results suggest the importance of a repair process in Chinese hamster V79 cells which is dependent upon cell growth rate and the dose of the mutagen for its effectiveness.", "contents": "Effect of cell growth rate and dose fractionation on chemically-induced  ouabain-resistant mutations in Chinese hamster V79 cells. Chinese hamster V79 cells were grown in medium containing either 10% or 2% FCS during the expression time following exposure to MNNG. The lower serum concentration was used to reduce the rate of cell replication, thereby allowing more time for DNA repair prior to \"fixation\" of the mutagenic lesion. In addition, fractionated and continuous exposures to MNNG and MAM, respectively, were carried out to determine their effect on the number of induced ouabain-resistant mutants. The results indicated that lowering the rate of cell growth effectively reduces the mutation frequency at low, but not at high doses of MNNG. Fractionated doses of MNNG result in a potentiation of their mutagenic effects compared to single doses. Also, continuous exposures to MAM result in an exponential increase in the mutation frequency. Collectively, these results suggest the importance of a repair process in Chinese hamster V79 cells which is dependent upon cell growth rate and the dose of the mutagen for its effectiveness."}
{"id": "PMID:460288", "title": "Selectivity of the excision of alkylation products in a xeroderma pigmentosum-derived lymphoblastoid line.", "content": "Lymphoblastoid cell derived from a complementation group C xeroderma patient were unable to remove 06-methyl guanine residues formed in DNA by treatment of cells with low concentration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The xeroderma cells were competent in their ability to excise 3-methyl adenine adducts. MNNG treatment induced excision repair in the xeroderma line and in addition the treatment resulted in the presence of numerous single-strand breaks in the DNA. The single gene, UV-excision-defective mutants of Escherichia coli, uvrA and uvrB, are able to excise MNNG-induced 06-methyl guanine adducts indicating that excision of this compound is not due to operation of UV endonuclease system.", "contents": "Selectivity of the excision of alkylation products in a xeroderma pigmentosum-derived lymphoblastoid line. Lymphoblastoid cell derived from a complementation group C xeroderma patient were unable to remove 06-methyl guanine residues formed in DNA by treatment of cells with low concentration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The xeroderma cells were competent in their ability to excise 3-methyl adenine adducts. MNNG treatment induced excision repair in the xeroderma line and in addition the treatment resulted in the presence of numerous single-strand breaks in the DNA. The single gene, UV-excision-defective mutants of Escherichia coli, uvrA and uvrB, are able to excise MNNG-induced 06-methyl guanine adducts indicating that excision of this compound is not due to operation of UV endonuclease system."}
{"id": "PMID:460292", "title": "Analysis of chromosome aberrations and sister-chromatid exchanges in human lymphocytes exposed in vitro to Hydergine.", "content": "Hydergine (dihydroergotoxine mesylate, Sandoz) was examined for its capability to induce chromosome damage and sister-chromatid exchanges (SCEs) in human lymphocyte in vitro. For the chromosome-aberration study, cultures set up from 6 individuals were divided into 5 groups: negative control, positive control (caffeine, 0.5 mg/ml), and Hydergine (0.1, 0.25 and 0.5 micrograms/ml). For the SCE examination, which used 8 individuals, 4 cultures were made per person in the following way: negative control, positive control (mitomycin C, 0.1 microgram/ml), and Hydergine (0.1 and 0.5 micrograms/ml). Lymphocytes were cultivated for 72 h, being exposed to the respective treatments during the final 24 h. The results showed that Hydergine induced no chromosome damage in human lymphocytes in vitro.", "contents": "Analysis of chromosome aberrations and sister-chromatid exchanges in human lymphocytes exposed in vitro to Hydergine. Hydergine (dihydroergotoxine mesylate, Sandoz) was examined for its capability to induce chromosome damage and sister-chromatid exchanges (SCEs) in human lymphocyte in vitro. For the chromosome-aberration study, cultures set up from 6 individuals were divided into 5 groups: negative control, positive control (caffeine, 0.5 mg/ml), and Hydergine (0.1, 0.25 and 0.5 micrograms/ml). For the SCE examination, which used 8 individuals, 4 cultures were made per person in the following way: negative control, positive control (mitomycin C, 0.1 microgram/ml), and Hydergine (0.1 and 0.5 micrograms/ml). Lymphocytes were cultivated for 72 h, being exposed to the respective treatments during the final 24 h. The results showed that Hydergine induced no chromosome damage in human lymphocytes in vitro."}
{"id": "PMID:460293", "title": "Induction of sister-chromatid exchanges in human lymphocytes and Chinese hamster cells exposed to aflatoxin B1 and N-methyl-N-nitrosourea.", "content": "The effect of a 1-h exposure to aflatoxin B1 (AFB1) in inducing sister-chromatid exchange in Chinese hamster ovary (CHO) cells and human lymphocytes in the presence or absence of mixed function oxidase (\"S9 mix\") was compared. CHO cells were also exposed to a graded series of doses of N-methyl-N-nitrosourea, a powerful inducer of SCE whose action was independent of the presence or absence of S9 mix. CHO and human cells showed a close correlation in response to SCE induction by AFB1 and in both cell systems the additon of mixed function oxidases in the S9 mix resulted in a marked enhancement of action of AFB1.", "contents": "Induction of sister-chromatid exchanges in human lymphocytes and Chinese hamster cells exposed to aflatoxin B1 and N-methyl-N-nitrosourea. The effect of a 1-h exposure to aflatoxin B1 (AFB1) in inducing sister-chromatid exchange in Chinese hamster ovary (CHO) cells and human lymphocytes in the presence or absence of mixed function oxidase (\"S9 mix\") was compared. CHO cells were also exposed to a graded series of doses of N-methyl-N-nitrosourea, a powerful inducer of SCE whose action was independent of the presence or absence of S9 mix. CHO and human cells showed a close correlation in response to SCE induction by AFB1 and in both cell systems the additon of mixed function oxidases in the S9 mix resulted in a marked enhancement of action of AFB1."}
{"id": "PMID:460294", "title": "The cytotoxic, mutagenic and clastogenic effects of chromium-containing compounds on mammalian cells in culture.", "content": "Examples of chromic and chromate salts have been examined for their effects on a cultured Chinese hamster cell line. The responses studied were cytotoxicity, mutagenesis and clastogenesis. Chromate (hexavalent chromium) salts of both high and medium water solubility were active in producing all three classes of response, whereas an insoluble chromate salt and a soluble chromic (trivalent chromium) salt were inactive. In addition to illustrating the value of using mammalian cells in culture for screening chemicals for biological activity, the results of this study reinforce current views regarding the genotoxic properties of chromates.", "contents": "The cytotoxic, mutagenic and clastogenic effects of chromium-containing compounds on mammalian cells in culture. Examples of chromic and chromate salts have been examined for their effects on a cultured Chinese hamster cell line. The responses studied were cytotoxicity, mutagenesis and clastogenesis. Chromate (hexavalent chromium) salts of both high and medium water solubility were active in producing all three classes of response, whereas an insoluble chromate salt and a soluble chromic (trivalent chromium) salt were inactive. In addition to illustrating the value of using mammalian cells in culture for screening chemicals for biological activity, the results of this study reinforce current views regarding the genotoxic properties of chromates."}
{"id": "PMID:460295", "title": "Platinum-induced mutations to 8-azaguanine resistance in Chinese hamster ovary cells.", "content": "6 platinum (Pt) compounds were compared in suspension cultured Chinese hamster ovary (CHO-S) cells with respect to their inhibition of growth, their reduction of cloning efficiency, and their induction of mutants resistant to 200 microM (30 micrograms/ml) 8-azaguanine (8-AG) and 3 mM ouabain (OUA), respectively. The toxicity of these compounds can be ranked by the medium concentrations which decrease suspension growth/or cloning efficiency by 50%: cis-Pt(NH3)2-Cl2 (0.9/1.5 microM) greater than Pt(SO4)2 + methylcobalamin (MeB-12) methylation product (20/10 microM) greater than K2PtCl4 (32/50 microM) = K2PtCl6 (34/50 microM) = MePtCl2-3 (60/50 microM) greater than Pt(SO4)2 (66/105 microM). Following 20 h exposures to concentrations which resulted in relative survivals of 80-2%, none of the foregoing compounds increased consistently the frequency of OUA(R) mutants above the spontaneous frequency (6.0 x 10(-6)). Parallel treatments with 800 microM (100 micrograms/ml) ethyl methanesulfonate (EMS) increased the OUA(R) mutant frequency 10--12-fold. Using 8-AG for mutant selection, dose-dependent increases of 5--7-fold above the spontaneous frequency (3--8 x 10(-5) were obtained with cis-Pt(NH3)2Cl2, Pt(S04)2, and the product from Pt(SO4)2 + MeB-12. Identical 20 h exposures to varying amounts of K2PtCl4, K2PtCl6, and MePtCl2-3 did not induce 8-AG(R) mutants. Optimal detection of Pt-induced 8-AG(R) mutants required 7 post-treatments, expression doublings in suspension culture. Under our selection conditions 8/8 spontaneous and 24/24 Pt-induced 8-AG(R) variants contained reduced hypoxanthine-guanine phosphoribosyl transferase (HGPRT) specific activities (means ranging from 3 to 11% of the parental CHO-S cells). When compared from linear plots of the 8-Ag(r) frequency against the initial medium concentration, cis-Pt(NH3)2Cl2 is 134 times and Pt(SO4)2 si 3.5 times more mutagenic than EMS. However, on a cell-survival basis EMS is 8--10-fold more mutagenic than these two Pt-compounds. 6-Thioguanine (10 microM) can be substituted for 8-AG to assay mutant induction by cis-Pt(NH3)2Cl2 and Pt(SO4)2 in CHO-S cells. The sensitivity of the CHO-S HGPRT locus for detecting mutagenesis by Pt complexes can be increased several fold by continuous subculture in the presence of these agents for 10--25 population doublings. By this procedure K2PtCl6 is seen to be weakly mutagenic and 20 microM Pt(SO4)2 produces 8-AG(R) mutants at frequencies requiring 7--8-fold higher concentrations when a fixed 20 h exposure is used.", "contents": "Platinum-induced mutations to 8-azaguanine resistance in Chinese hamster ovary cells. 6 platinum (Pt) compounds were compared in suspension cultured Chinese hamster ovary (CHO-S) cells with respect to their inhibition of growth, their reduction of cloning efficiency, and their induction of mutants resistant to 200 microM (30 micrograms/ml) 8-azaguanine (8-AG) and 3 mM ouabain (OUA), respectively. The toxicity of these compounds can be ranked by the medium concentrations which decrease suspension growth/or cloning efficiency by 50%: cis-Pt(NH3)2-Cl2 (0.9/1.5 microM) greater than Pt(SO4)2 + methylcobalamin (MeB-12) methylation product (20/10 microM) greater than K2PtCl4 (32/50 microM) = K2PtCl6 (34/50 microM) = MePtCl2-3 (60/50 microM) greater than Pt(SO4)2 (66/105 microM). Following 20 h exposures to concentrations which resulted in relative survivals of 80-2%, none of the foregoing compounds increased consistently the frequency of OUA(R) mutants above the spontaneous frequency (6.0 x 10(-6)). Parallel treatments with 800 microM (100 micrograms/ml) ethyl methanesulfonate (EMS) increased the OUA(R) mutant frequency 10--12-fold. Using 8-AG for mutant selection, dose-dependent increases of 5--7-fold above the spontaneous frequency (3--8 x 10(-5) were obtained with cis-Pt(NH3)2Cl2, Pt(S04)2, and the product from Pt(SO4)2 + MeB-12. Identical 20 h exposures to varying amounts of K2PtCl4, K2PtCl6, and MePtCl2-3 did not induce 8-AG(R) mutants. Optimal detection of Pt-induced 8-AG(R) mutants required 7 post-treatments, expression doublings in suspension culture. Under our selection conditions 8/8 spontaneous and 24/24 Pt-induced 8-AG(R) variants contained reduced hypoxanthine-guanine phosphoribosyl transferase (HGPRT) specific activities (means ranging from 3 to 11% of the parental CHO-S cells). When compared from linear plots of the 8-Ag(r) frequency against the initial medium concentration, cis-Pt(NH3)2Cl2 is 134 times and Pt(SO4)2 si 3.5 times more mutagenic than EMS. However, on a cell-survival basis EMS is 8--10-fold more mutagenic than these two Pt-compounds. 6-Thioguanine (10 microM) can be substituted for 8-AG to assay mutant induction by cis-Pt(NH3)2Cl2 and Pt(SO4)2 in CHO-S cells. The sensitivity of the CHO-S HGPRT locus for detecting mutagenesis by Pt complexes can be increased several fold by continuous subculture in the presence of these agents for 10--25 population doublings. By this procedure K2PtCl6 is seen to be weakly mutagenic and 20 microM Pt(SO4)2 produces 8-AG(R) mutants at frequencies requiring 7--8-fold higher concentrations when a fixed 20 h exposure is used."}
{"id": "PMID:460304", "title": "Lack of evidence for cancer due to use of metronidazole.", "content": "Experimental studies have shown that metronidazole is carcinogenic in rodents and mutagenic in bacteria. In 771 women given metronidazole for the treatment of vaginal trichomoniasis, more cancers developed than had been expected after exclusion of carcinoma of one uterine cervix (observed, 24 cases; expected on the basis of the Connecticut Tumor Registry, 21.7; expected on the basis of the Third National Cancer Survey, 18.4). However, the excess was not statistically significant (P greater than 0.05). The observed and expected numbers of breast-cancer cases were the same, but four lung-cancer cases were observed, whereas 0.6 would have been expected. This finding is confounded by the fact that all four lung cancers developed in women who were smokers. Overall, we observed no appreciable increase in cases of cancer.", "contents": "Lack of evidence for cancer due to use of metronidazole. Experimental studies have shown that metronidazole is carcinogenic in rodents and mutagenic in bacteria. In 771 women given metronidazole for the treatment of vaginal trichomoniasis, more cancers developed than had been expected after exclusion of carcinoma of one uterine cervix (observed, 24 cases; expected on the basis of the Connecticut Tumor Registry, 21.7; expected on the basis of the Third National Cancer Survey, 18.4). However, the excess was not statistically significant (P greater than 0.05). The observed and expected numbers of breast-cancer cases were the same, but four lung-cancer cases were observed, whereas 0.6 would have been expected. This finding is confounded by the fact that all four lung cancers developed in women who were smokers. Overall, we observed no appreciable increase in cases of cancer."}
{"id": "PMID:460305", "title": "Red-cell-membrane polypeptide aggregates in glucose-6-phosphate dehydrogenase mutants with chronic hemolytic disease. A clue to the mechanism of hemolysis.", "content": "Red-cell membranes from patients with glucose-6-phosphate dehydrogenase deficiency were studied with polyacrylamide gel electrophoresis and gel filtration chromatography in sodium dodecyl sulfate. Membranes from each of five such patients who also had chronic hemolytic disease contained polypeptide aggregates within two molecular-weight ranges (4.4 X 10(5) and greater than 50 X 10(6) daltons). The 4.4 X 10(5) dalton aggregates were not detectable in red-cell membranes of patients with the enzyme deficiency without chronic hemolysis or in membranes from normal subjects, and the greater than 50 X 10(6) dalton aggregates were not found in appreciable amounts in these cells. The aggregates were dissociated by mercaptoethanol or dithiothreitol -- indicating that they were formed by intermolecular disulfide bonds. The polypeptide aggregates contained spectrin but not globin. Red-cell deformability was decreased in aggregate-containing cells. We postulate that the polypeptide aggregates are indicators of oxidant damage to the red-cell membrane, which results in decreased deformability and chronic hemolysis.", "contents": "Red-cell-membrane polypeptide aggregates in glucose-6-phosphate dehydrogenase mutants with chronic hemolytic disease. A clue to the mechanism of hemolysis. Red-cell membranes from patients with glucose-6-phosphate dehydrogenase deficiency were studied with polyacrylamide gel electrophoresis and gel filtration chromatography in sodium dodecyl sulfate. Membranes from each of five such patients who also had chronic hemolytic disease contained polypeptide aggregates within two molecular-weight ranges (4.4 X 10(5) and greater than 50 X 10(6) daltons). The 4.4 X 10(5) dalton aggregates were not detectable in red-cell membranes of patients with the enzyme deficiency without chronic hemolysis or in membranes from normal subjects, and the greater than 50 X 10(6) dalton aggregates were not found in appreciable amounts in these cells. The aggregates were dissociated by mercaptoethanol or dithiothreitol -- indicating that they were formed by intermolecular disulfide bonds. The polypeptide aggregates contained spectrin but not globin. Red-cell deformability was decreased in aggregate-containing cells. We postulate that the polypeptide aggregates are indicators of oxidant damage to the red-cell membrane, which results in decreased deformability and chronic hemolysis."}
{"id": "PMID:460322", "title": "Prognostic value of exercise testing soon after myocardial infarction.", "content": "The prognostic value of a limited treadmill exercises test performed one day before hospital discharge after acute myocardial infarction was studied in 210 consecutive patients who had no over heart failure and had been free of chest pain for at least four days. No complications occurred. During a one-year follow-up period 28 of 43 patients (65 per cent) who had chest pain during the test reported angina, as compared with 60 of 167 (36 per cent) who had no chest pain during test (P less than 0.001). The one-year mortality rates were 2.1 per cent (three of 146) in patients without changes in the S-T segment during exercise and 27 per cent (17 of 64) in those with depression of the S-T segment (P less than 0.001). Sudden death occurred in one of 146 (0.7 per cent) patients who showed no change in the S-T segment and in 10 of 64 (16 per cent) with depression of the segment (P less than 0.001). Thus, a limited treadmill exercise test performed before hospital discharge after acute myocardial infarction is safe and can predict mortality in the subsequent year.", "contents": "Prognostic value of exercise testing soon after myocardial infarction. The prognostic value of a limited treadmill exercises test performed one day before hospital discharge after acute myocardial infarction was studied in 210 consecutive patients who had no over heart failure and had been free of chest pain for at least four days. No complications occurred. During a one-year follow-up period 28 of 43 patients (65 per cent) who had chest pain during the test reported angina, as compared with 60 of 167 (36 per cent) who had no chest pain during test (P less than 0.001). The one-year mortality rates were 2.1 per cent (three of 146) in patients without changes in the S-T segment during exercise and 27 per cent (17 of 64) in those with depression of the S-T segment (P less than 0.001). Sudden death occurred in one of 146 (0.7 per cent) patients who showed no change in the S-T segment and in 10 of 64 (16 per cent) with depression of the segment (P less than 0.001). Thus, a limited treadmill exercise test performed before hospital discharge after acute myocardial infarction is safe and can predict mortality in the subsequent year."}
{"id": "PMID:460323", "title": "Use of antimicrobial drugs in general hospitals: patterns of prophylaxis.", "content": "The patterns of prophylactic use of antimicrobial drugs were reviewed in 5288 charts drawn by a random method from 20 randomly selected short-stay general hospitals in Pennsylvania. About 10 per cent of hospitalized patients received antimicrobial drugs for prophylaxis in operations or nonsurgical procedures, and prophylaxis accounted for about 30 per cent of all antimicrobial drugs administered in hospitals. The drugs used most often for prophylaxis were cephalosporins, followed by benzyl penicillins,  ampicillin and tetracyclines, in that order. Despite indications that prophylaxis, when useful at all, is effective only when given concurrently with and for 24 to 48 hours after operation, it was usually continued throughout hospitalization. Almost 80 per cent of prophylactic antimicrobial drugs were administered at least 48 hours after an operation or procedure -- suggesting that limiting prophylaxis to the first 24 to 48 hours, as currently recommended, would substantially reduce expenditures for antimicrobial drugs in hospitals.", "contents": "Use of antimicrobial drugs in general hospitals: patterns of prophylaxis. The patterns of prophylactic use of antimicrobial drugs were reviewed in 5288 charts drawn by a random method from 20 randomly selected short-stay general hospitals in Pennsylvania. About 10 per cent of hospitalized patients received antimicrobial drugs for prophylaxis in operations or nonsurgical procedures, and prophylaxis accounted for about 30 per cent of all antimicrobial drugs administered in hospitals. The drugs used most often for prophylaxis were cephalosporins, followed by benzyl penicillins,  ampicillin and tetracyclines, in that order. Despite indications that prophylaxis, when useful at all, is effective only when given concurrently with and for 24 to 48 hours after operation, it was usually continued throughout hospitalization. Almost 80 per cent of prophylactic antimicrobial drugs were administered at least 48 hours after an operation or procedure -- suggesting that limiting prophylaxis to the first 24 to 48 hours, as currently recommended, would substantially reduce expenditures for antimicrobial drugs in hospitals."}
{"id": "PMID:460339", "title": "Hepatitis after the transfusion of frozen red cells and washed red cells.", "content": "The large-scale use of frozen red cells and washed red cells in this transfusion service has not been effective in reducing post-transfusion hepatitis. Seventy-eight per cent of 31,125 transfusions, excluding platelets and plasma, were given in the form of frozen or washed red cells. These transfusions were associated with 56 cases of hepatitis. In 37 cases, the recipients had received only frozen or washed red cells (or both). Post-transfusion hepatitis was of the non-A, non-B type in 95% of cases. On average, commercial blood accounted for 30 per cent of the donor base during the first four years and was involved in 44 of the 56 cases. Elimination of commercial blood from the donor base resulted in a decrease in the incidence of hepatitis from 2.1 to 0.3 per 1000 transfusions.", "contents": "Hepatitis after the transfusion of frozen red cells and washed red cells. The large-scale use of frozen red cells and washed red cells in this transfusion service has not been effective in reducing post-transfusion hepatitis. Seventy-eight per cent of 31,125 transfusions, excluding platelets and plasma, were given in the form of frozen or washed red cells. These transfusions were associated with 56 cases of hepatitis. In 37 cases, the recipients had received only frozen or washed red cells (or both). Post-transfusion hepatitis was of the non-A, non-B type in 95% of cases. On average, commercial blood accounted for 30 per cent of the donor base during the first four years and was involved in 44 of the 56 cases. Elimination of commercial blood from the donor base resulted in a decrease in the incidence of hepatitis from 2.1 to 0.3 per 1000 transfusions."}
{"id": "PMID:460340", "title": "Natural history of bacteriuria in schoolgirls. A long-term case-control study.", "content": "To define better the natural history of bacteriuria in females, we followed 60 schoolgirls with bacteriuria and 38 matched controls for periods ranging from nine to 18 years. Among the schoolgirls with bacteriuria (greater than or equal to 10(5) organisms per milliliter in two or more consecutive cultures), reflux was repaired in five, nephrectomy was performed in two, and reduced inulin clearance was noted in one subject with atrophic pyelonephritis. Serum creatinine was slightly higher in cases than in controls. Renal scars or caliectasis occurred in 16 cases but in none of the controls. Blood pressure was similar in both groups. Episodes of bacteriuria in cases and controls were, respectively: five or more episodes, 21.7 and 2.6 per cent; and episodes during pregnancy, 63.8 and 26.7 per cent. Seven children of the cases but none of the children of controls showed urinary-tract infections. Bacteriuria among schoolgirls defines a group at great risk of recurrent symptomatic infections and renal scars and at low risk of reduced renal function.", "contents": "Natural history of bacteriuria in schoolgirls. A long-term case-control study. To define better the natural history of bacteriuria in females, we followed 60 schoolgirls with bacteriuria and 38 matched controls for periods ranging from nine to 18 years. Among the schoolgirls with bacteriuria (greater than or equal to 10(5) organisms per milliliter in two or more consecutive cultures), reflux was repaired in five, nephrectomy was performed in two, and reduced inulin clearance was noted in one subject with atrophic pyelonephritis. Serum creatinine was slightly higher in cases than in controls. Renal scars or caliectasis occurred in 16 cases but in none of the controls. Blood pressure was similar in both groups. Episodes of bacteriuria in cases and controls were, respectively: five or more episodes, 21.7 and 2.6 per cent; and episodes during pregnancy, 63.8 and 26.7 per cent. Seven children of the cases but none of the children of controls showed urinary-tract infections. Bacteriuria among schoolgirls defines a group at great risk of recurrent symptomatic infections and renal scars and at low risk of reduced renal function."}
{"id": "PMID:460341", "title": "Quinidine-digoxin interaction: Pharmacokinetics, underlying mechanism and clinical implications.", "content": "Administration of quinidine with digoxin increased serum digoxin concentrations in 79 patients and five volunteers. In 38 patients on a constant glycoside maintenance dose, the addition of quinidine to digoxin therapy resulted in a mean 2.5-fold increase (from 0.98 +/- 0.37 to 2.47 +/- 0.71 ng per milliliter, mean +/- 1 S.D.) (P less than 0.001). The addition of quinidine decreased renal glycoside clearance (from 91.6 +/- 27.8 to 40.6 +/- 15.8 ml per minute) (P less than 0.001). Unlike other investigations, our studies provided no evidence that quinidine displaced digoxin at specific cardiac binding sites. The elevated digoxin levels found during quinidine administration suggest a 30 to 50 per cent reduction of the digoxin dose. Adverse reactions to combined quinidine-digoxin therapy may be partly due to digitalis intoxication.", "contents": "Quinidine-digoxin interaction: Pharmacokinetics, underlying mechanism and clinical implications. Administration of quinidine with digoxin increased serum digoxin concentrations in 79 patients and five volunteers. In 38 patients on a constant glycoside maintenance dose, the addition of quinidine to digoxin therapy resulted in a mean 2.5-fold increase (from 0.98 +/- 0.37 to 2.47 +/- 0.71 ng per milliliter, mean +/- 1 S.D.) (P less than 0.001). The addition of quinidine decreased renal glycoside clearance (from 91.6 +/- 27.8 to 40.6 +/- 15.8 ml per minute) (P less than 0.001). Unlike other investigations, our studies provided no evidence that quinidine displaced digoxin at specific cardiac binding sites. The elevated digoxin levels found during quinidine administration suggest a 30 to 50 per cent reduction of the digoxin dose. Adverse reactions to combined quinidine-digoxin therapy may be partly due to digitalis intoxication."}
{"id": "PMID:460363", "title": "Effect of intrathoracic pressure on left ventricular performance.", "content": "Left ventricular dysfunction is common in respiratory-distress syndrome, asthma and obstructive lung disease. To understand the contribution of intrathoracic pressure to this problem, we studied the effects of Valsalva and M\u00fcller maneuvers on left ventricular function in eight patients. Implantation of intramyocardial markers permitted beat-by-beat measurement of the velocity of fiber shortening (VCF) and left ventricular volume. During the M\u00fcller maneuver, VCF and ejection fraction decreased despite an increase in left ventricular volume and a decline in arterial pressure. In addition, when arterial pressure was corrected for changes in intrapleural pressure during either maneuver it correlated better with left ventricular end-systolic volumes than did uncorrected arterial pressures. These findings suggest that negative intrathoracic pressure affects left ventricular function by increasing left ventricular transmural pressures and thus afterload. We conclude that large intrathoracic-pressure changes, such as those that occur in acute pulmonary disease, can influence cardiac performance.", "contents": "Effect of intrathoracic pressure on left ventricular performance. Left ventricular dysfunction is common in respiratory-distress syndrome, asthma and obstructive lung disease. To understand the contribution of intrathoracic pressure to this problem, we studied the effects of Valsalva and M\u00fcller maneuvers on left ventricular function in eight patients. Implantation of intramyocardial markers permitted beat-by-beat measurement of the velocity of fiber shortening (VCF) and left ventricular volume. During the M\u00fcller maneuver, VCF and ejection fraction decreased despite an increase in left ventricular volume and a decline in arterial pressure. In addition, when arterial pressure was corrected for changes in intrapleural pressure during either maneuver it correlated better with left ventricular end-systolic volumes than did uncorrected arterial pressures. These findings suggest that negative intrathoracic pressure affects left ventricular function by increasing left ventricular transmural pressures and thus afterload. We conclude that large intrathoracic-pressure changes, such as those that occur in acute pulmonary disease, can influence cardiac performance."}
{"id": "PMID:460364", "title": "Mechanisms of edema formation in myxedema--increased protein extravasation and relatively slow lymphatic drainage.", "content": "We assessed extravascular accumulation of albumin and fluid in primary myxedema by measuring metabolic turnover and transcapillary escape of 131I-labeled human albumin in seven patients. In the hypothyroid state, we found a low plasma volume (P less than 0.05), a reduced rate of albumin synthesis and catabolism (P less than 0.01), an increased transcapillary escape rate of albumin (P less than 0.01), a remarkable increase in the extravascular mass of albumin (1500 micronmol; P less than 0.01) and a longer mean transit time through the extravascular spaces in primary myxedema than in other states of generalized edema (P less than 0.05). All variables returned to normal during l-thyroxine treatment. The extravascular accumulation of albumin, and presumably of all other plasma proteins, is important in the generalized edema typically found in myxedema. Inadequate lymphatic drainage may also explain the formation of exudates in the serous cavities that are well known in myxedema.", "contents": "Mechanisms of edema formation in myxedema--increased protein extravasation and relatively slow lymphatic drainage. We assessed extravascular accumulation of albumin and fluid in primary myxedema by measuring metabolic turnover and transcapillary escape of 131I-labeled human albumin in seven patients. In the hypothyroid state, we found a low plasma volume (P less than 0.05), a reduced rate of albumin synthesis and catabolism (P less than 0.01), an increased transcapillary escape rate of albumin (P less than 0.01), a remarkable increase in the extravascular mass of albumin (1500 micronmol; P less than 0.01) and a longer mean transit time through the extravascular spaces in primary myxedema than in other states of generalized edema (P less than 0.05). All variables returned to normal during l-thyroxine treatment. The extravascular accumulation of albumin, and presumably of all other plasma proteins, is important in the generalized edema typically found in myxedema. Inadequate lymphatic drainage may also explain the formation of exudates in the serous cavities that are well known in myxedema."}
{"id": "PMID:460393", "title": "[Does nutrition influence the propagation of neoplasms?].", "content": "In the last years, questions concerning the relationship between nutrition and the occurrence of neoplasms have been studied more intensively. The author discusses the possible involvement of water as a potential carrier of certain cylic hydrocarbons and radionuclides, and of nutrition per se. He treats problems related to, inter alia, bulk materials, polyethenoid fatty acids, the increase in fat consumption, trace elements, vitamins and the importance of the intestinal flora.", "contents": "[Does nutrition influence the propagation of neoplasms?]. In the last years, questions concerning the relationship between nutrition and the occurrence of neoplasms have been studied more intensively. The author discusses the possible involvement of water as a potential carrier of certain cylic hydrocarbons and radionuclides, and of nutrition per se. He treats problems related to, inter alia, bulk materials, polyethenoid fatty acids, the increase in fat consumption, trace elements, vitamins and the importance of the intestinal flora."}
{"id": "PMID:460394", "title": "[Determination of aluminum in foods].", "content": "In connection with studies on the changes of functional properties of food constituents (e. g. solubility of proteins) by means of aluminium compounds, the elaboration of an appropriate method for the quantitative determination of aluminium has been necessary. The organic samples are mineralized with 80% perchloric acid; in case of fat-containing foods, after fat removal. After reaction with aluminon (ammonium salt of aurin tricarboxylic acid), aluminium is determined photometrically at 530 nm. The limit of detection lies between 1 and 2 ppm; the recovery rate is 103%; the standard deviation is +/- 10%. The determination (without mineralization) requires about 1 hours. The authors analyzed, inter alia, milk (1.2--1.7 ppm A1), fat cheese (9 ppm), micora (4 ppm), aluminium-stabilized protein texturates (300--1500 ppm).", "contents": "[Determination of aluminum in foods]. In connection with studies on the changes of functional properties of food constituents (e. g. solubility of proteins) by means of aluminium compounds, the elaboration of an appropriate method for the quantitative determination of aluminium has been necessary. The organic samples are mineralized with 80% perchloric acid; in case of fat-containing foods, after fat removal. After reaction with aluminon (ammonium salt of aurin tricarboxylic acid), aluminium is determined photometrically at 530 nm. The limit of detection lies between 1 and 2 ppm; the recovery rate is 103%; the standard deviation is +/- 10%. The determination (without mineralization) requires about 1 hours. The authors analyzed, inter alia, milk (1.2--1.7 ppm A1), fat cheese (9 ppm), micora (4 ppm), aluminium-stabilized protein texturates (300--1500 ppm)."}
{"id": "PMID:460395", "title": "[Milk proteins for food. I. Some aspects of the use of milk proteins for the protein supply to the population of the GDR].", "content": "Milk protein accounts for approximately 21% of the protein supply to the population in the GDR. All milk protein fractions have a high nutritional-physiological value and favourable functional properties. This notwithstanding, only 50% of the milk protein processed by the dairy industry are at present employed in human nutrition, the remainder is used as a provender. The reasons for this situation are discussed. Principles of procedures for the industrial production of milk protein concentrates are presented.", "contents": "[Milk proteins for food. I. Some aspects of the use of milk proteins for the protein supply to the population of the GDR]. Milk protein accounts for approximately 21% of the protein supply to the population in the GDR. All milk protein fractions have a high nutritional-physiological value and favourable functional properties. This notwithstanding, only 50% of the milk protein processed by the dairy industry are at present employed in human nutrition, the remainder is used as a provender. The reasons for this situation are discussed. Principles of procedures for the industrial production of milk protein concentrates are presented."}
{"id": "PMID:460396", "title": "[Substrate specificity of a protease from Thermoactinomyces vulgaris].", "content": "The cleavage specificity of a protease from Thermoactinomyces vulgaris (thermitase) was determined by the insulin B-chain and the cleavability of casein and haemoglobin by this enzyme as compared to other proteases (trypsin, chymotrypsin, proteases from Bac. megaterium and cytophages). The most intense splitting effect on the substrates under investigation (insulin B-chain, casein and haemoglobin) is exerted by thermitase, i. e., the unspecificity of this enzyme is especially marked.", "contents": "[Substrate specificity of a protease from Thermoactinomyces vulgaris]. The cleavage specificity of a protease from Thermoactinomyces vulgaris (thermitase) was determined by the insulin B-chain and the cleavability of casein and haemoglobin by this enzyme as compared to other proteases (trypsin, chymotrypsin, proteases from Bac. megaterium and cytophages). The most intense splitting effect on the substrates under investigation (insulin B-chain, casein and haemoglobin) is exerted by thermitase, i. e., the unspecificity of this enzyme is especially marked."}
{"id": "PMID:460397", "title": "[Interactions of toxic trace elements with special regard to selenium].", "content": "The significance of the trace elements in nutrition is often decisively affected by the interaction of trace elements with other trace elements and of trace elements with other substances. These processes, which include synergistic and antagonistic effects, are of great importance not only to the essentiality, but also to the toxicity of trace elements. There is considerable experience with interactions of essential elements. There is considerable experience with interactions of essential elements or of elements in essential concentrations. On contrast, there is a scarcity of information pertaining to toxic effects that are influenced by synergisms and antagonisms. This situation is illustrated by some examples and especially by the role of selenium. It is shown that these problems deserve closer attention.", "contents": "[Interactions of toxic trace elements with special regard to selenium]. The significance of the trace elements in nutrition is often decisively affected by the interaction of trace elements with other trace elements and of trace elements with other substances. These processes, which include synergistic and antagonistic effects, are of great importance not only to the essentiality, but also to the toxicity of trace elements. There is considerable experience with interactions of essential elements. There is considerable experience with interactions of essential elements or of elements in essential concentrations. On contrast, there is a scarcity of information pertaining to toxic effects that are influenced by synergisms and antagonisms. This situation is illustrated by some examples and especially by the role of selenium. It is shown that these problems deserve closer attention."}
{"id": "PMID:460398", "title": "[Sweetness potency of saccharin and cyclamate in hot drinks].", "content": "The sweetness potency of sweeteners depends on temperature. At 50 degrees C the 2.4-fold concentration of saccharin and the 1.3-fold of cyclamate respectively is needed to produce the same sweetness (as related to sucrose) as in solutions at 20 degrees C. As well as in water the sweetening power decreases in chinese tea with increasing temperature. Only in case of cyclamate solutions of high sweetness this effect is diminished. In the mathematical description of the relation between stimulus and perception the decrease of the relative sweetness observed can be expressed by a comformable decrease of the concentration coefficient. The effect of temperature on the perception of sweetness can be explained by the Arrhenius' conception. The critical increments are 23 kJ/mol for saccharin and 8 kJ/mol for both cyclamate and fructose.", "contents": "[Sweetness potency of saccharin and cyclamate in hot drinks]. The sweetness potency of sweeteners depends on temperature. At 50 degrees C the 2.4-fold concentration of saccharin and the 1.3-fold of cyclamate respectively is needed to produce the same sweetness (as related to sucrose) as in solutions at 20 degrees C. As well as in water the sweetening power decreases in chinese tea with increasing temperature. Only in case of cyclamate solutions of high sweetness this effect is diminished. In the mathematical description of the relation between stimulus and perception the decrease of the relative sweetness observed can be expressed by a comformable decrease of the concentration coefficient. The effect of temperature on the perception of sweetness can be explained by the Arrhenius' conception. The critical increments are 23 kJ/mol for saccharin and 8 kJ/mol for both cyclamate and fructose."}
{"id": "PMID:460399", "title": "[Methodological bases of the nutritional-physiological assessment of the per capita consumption of foods. I. Recording and characterization of the per capita consumption].", "content": "Per capita consumption indices are reference points essential to the provision of foods and the planning of food consumption. From them global statements regarding the energy and nutrient supply can be deduced. But this requires the exact definition of the per capita consumption indices with regard to the recording level, subitems and average losses. The mode of calculation and the assortment of each of the presented food groups are characterized in detail and estimate values for the occurring losses are indicated which are needed for the nutritional-physiological balancing of the per capita consumption indices.", "contents": "[Methodological bases of the nutritional-physiological assessment of the per capita consumption of foods. I. Recording and characterization of the per capita consumption]. Per capita consumption indices are reference points essential to the provision of foods and the planning of food consumption. From them global statements regarding the energy and nutrient supply can be deduced. But this requires the exact definition of the per capita consumption indices with regard to the recording level, subitems and average losses. The mode of calculation and the assortment of each of the presented food groups are characterized in detail and estimate values for the occurring losses are indicated which are needed for the nutritional-physiological balancing of the per capita consumption indices."}
{"id": "PMID:460401", "title": "Statistical mechanics of supercoils and the torsional stiffness of the DNA double helix.", "content": "The distribution of closed unknotted polymer chains over the writhing number is calculated by the Monte-Carlo method. For circular duplex DNA the variance of the distribution equals approximately half the observed variance of equilibrium distribution over the linking number. The balance which arises from fluctuations in DNA twisting makes it possible to estimate the torsional stiffness of the double helix.", "contents": "Statistical mechanics of supercoils and the torsional stiffness of the DNA double helix. The distribution of closed unknotted polymer chains over the writhing number is calculated by the Monte-Carlo method. For circular duplex DNA the variance of the distribution equals approximately half the observed variance of equilibrium distribution over the linking number. The balance which arises from fluctuations in DNA twisting makes it possible to estimate the torsional stiffness of the double helix."}
{"id": "PMID:460412", "title": "Population biology of infectious diseases: Part I.", "content": "If the host population is taken to be a dynamic variable (rather than constant, as conventionally assumed), a wider understanding of the population biology of infectious diseases emerges. In this first part of a two-part article, mathematical models are developed, shown to fit data from laboratory experiments, and used to explore the evolutionary relations among transmission parameters. In the second part of the article, to be published in next week's issue, the models are extended to include indirectly transmitted infections, and the general implications for infectious diseases are considered.", "contents": "Population biology of infectious diseases: Part I. If the host population is taken to be a dynamic variable (rather than constant, as conventionally assumed), a wider understanding of the population biology of infectious diseases emerges. In this first part of a two-part article, mathematical models are developed, shown to fit data from laboratory experiments, and used to explore the evolutionary relations among transmission parameters. In the second part of the article, to be published in next week's issue, the models are extended to include indirectly transmitted infections, and the general implications for infectious diseases are considered."}
{"id": "PMID:460424", "title": "Population biology of infectious diseases: Part II.", "content": "In the first part of this two-part article (Nature 280, 361--367), mathematical models of directly transmitted microparasitic infections were developed, taking explicit account of the dynamics of the host population. The discussion is now extended to both microparasites (viruses, bacteria and protozoa) and macroparasites (helminths and arthropods), transmitted either directly or indirectly via one or more intermediate hosts. Consideration is given to the relation between the ecology and evolution of the transmission processes and the overall dynamics, and to the mechanisms that can produce cyclic patterns, or multiple stable states, in the levels of infection in the host population.", "contents": "Population biology of infectious diseases: Part II. In the first part of this two-part article (Nature 280, 361--367), mathematical models of directly transmitted microparasitic infections were developed, taking explicit account of the dynamics of the host population. The discussion is now extended to both microparasites (viruses, bacteria and protozoa) and macroparasites (helminths and arthropods), transmitted either directly or indirectly via one or more intermediate hosts. Consideration is given to the relation between the ecology and evolution of the transmission processes and the overall dynamics, and to the mechanisms that can produce cyclic patterns, or multiple stable states, in the levels of infection in the host population."}
{"id": "PMID:460425", "title": "The amino acid sequence of the alpha-chain of human fibrinogen.", "content": "The amino acid sequence of the human fibrinogen alpha-chain reveals a structure that can be divided into three zones of unique amino acid composition. The middle of these contains the two primary alpha-chain cross-linking acceptor sites and consists of a remarkable series of internal duplications.", "contents": "The amino acid sequence of the alpha-chain of human fibrinogen. The amino acid sequence of the human fibrinogen alpha-chain reveals a structure that can be divided into three zones of unique amino acid composition. The middle of these contains the two primary alpha-chain cross-linking acceptor sites and consists of a remarkable series of internal duplications."}
{"id": "PMID:460437", "title": "Temperature-dependent X-ray diffraction as a probe of protein structural dynamics.", "content": "X-ray diffraction at four temperatures from 220 to 300 K coupled with crystallographic refinement yields the mean-square displacements and conformational potentials of all 1,261 non-hydrogen atoms of metmyoglobin. The results are interpreted to indicate a condensed core around the haem, semi-liquid regions towards the outside and a possible pathway for ligands. It is concluded that X-ray diffraction can provide the spatial distribution of the dynamic features of a protein.", "contents": "Temperature-dependent X-ray diffraction as a probe of protein structural dynamics. X-ray diffraction at four temperatures from 220 to 300 K coupled with crystallographic refinement yields the mean-square displacements and conformational potentials of all 1,261 non-hydrogen atoms of metmyoglobin. The results are interpreted to indicate a condensed core around the haem, semi-liquid regions towards the outside and a possible pathway for ligands. It is concluded that X-ray diffraction can provide the spatial distribution of the dynamic features of a protein."}
{"id": "PMID:460438", "title": "Crystallographic studies of the dynamic properties of lysozyme.", "content": "The patterns of atomic displacements in the crystals of hen and human lysozyme derived from independent crystallographic refinement are broadly similar. Analysis of the pattern indicates a close correlation with molecular structure, strongly suggestive of intramolecular motion. The active site of lysozyme is located in a region of high displacement. It is concluded that protein mobility may play a significant part in biological activity and that X-ray crystallography can contribute to its analysis.", "contents": "Crystallographic studies of the dynamic properties of lysozyme. The patterns of atomic displacements in the crystals of hen and human lysozyme derived from independent crystallographic refinement are broadly similar. Analysis of the pattern indicates a close correlation with molecular structure, strongly suggestive of intramolecular motion. The active site of lysozyme is located in a region of high displacement. It is concluded that protein mobility may play a significant part in biological activity and that X-ray crystallography can contribute to its analysis."}
{"id": "PMID:460490", "title": "[Participation of the lateral geniculate body in color discrimination in cats].", "content": "Responses of the lateral geniculate units to different wave length stimuli were studied in immobilized cats. The studied units were divided into two groups. Those of the first group (44%) produce high-frequency spike discharges which are not modified when the wave length of stimulus changes. Statistically significant differences are found in the unit responses of the second group (51%) to different wave length stimuli. A possible inhibitory influence of the first group units on those of the second group is suggested. The possible significance of this influence in the colour vision is discussed.", "contents": "[Participation of the lateral geniculate body in color discrimination in cats]. Responses of the lateral geniculate units to different wave length stimuli were studied in immobilized cats. The studied units were divided into two groups. Those of the first group (44%) produce high-frequency spike discharges which are not modified when the wave length of stimulus changes. Statistically significant differences are found in the unit responses of the second group (51%) to different wave length stimuli. A possible inhibitory influence of the first group units on those of the second group is suggested. The possible significance of this influence in the colour vision is discussed."}
{"id": "PMID:460491", "title": "[Statistical analysis of the functional features of cat motor cortex neurons].", "content": "Recordings were made from 366 neurons situated in the motor area of m.biceps in the cortex of 6 cats trained to make instrumental placing reaction and to accept manipulation of the joints and natural cutaneous stimulation of the forelimb. From 160 neurons whose discharges changed during placing reaction performance 52 (32.5%) were demonstrated to receive an input from peripheral receptors in the forelimb. In contrast only 7 (3.4%) neurons in the group not related to the movement received such an input. From 160 neurons 31 changed discharge before movement onset, 59 after movement onset, and 70 before and during movement. Correlation between functional types of the neurons and their peripheral inputs was found.", "contents": "[Statistical analysis of the functional features of cat motor cortex neurons]. Recordings were made from 366 neurons situated in the motor area of m.biceps in the cortex of 6 cats trained to make instrumental placing reaction and to accept manipulation of the joints and natural cutaneous stimulation of the forelimb. From 160 neurons whose discharges changed during placing reaction performance 52 (32.5%) were demonstrated to receive an input from peripheral receptors in the forelimb. In contrast only 7 (3.4%) neurons in the group not related to the movement received such an input. From 160 neurons 31 changed discharge before movement onset, 59 after movement onset, and 70 before and during movement. Correlation between functional types of the neurons and their peripheral inputs was found."}
{"id": "PMID:460492", "title": "[Functional characteristics of connections: Schaffer's collaterals to the CA1 field of the hippocampus in experiments in vivo and in vitro].", "content": "Comparative analysis of functional characteristics of connection between the fields CA3 and CA1 (Schaffer's collaterals) was performed in experiments in vivo (unanaesthetized rabbits) and in vitro (hippocampal slices of guinea-pigs) with extracellular recording of the unitary activity in the field CA1. Weakness of postexcitatory inhibition, absence of responses of the form of suppression of spontaneous activity, higher effectiveness of low-frequency stimulation of Schaffer's collaterals were observed in experiments in vitro. Posttetanic effects were more frequently observed and lasted longer in vitro than in vivo. The dominating effect in vivo was posttetanic depression and in vitro--posttetanic potentiation. The possible reasons for these differences are discussed.", "contents": "[Functional characteristics of connections: Schaffer's collaterals to the CA1 field of the hippocampus in experiments in vivo and in vitro]. Comparative analysis of functional characteristics of connection between the fields CA3 and CA1 (Schaffer's collaterals) was performed in experiments in vivo (unanaesthetized rabbits) and in vitro (hippocampal slices of guinea-pigs) with extracellular recording of the unitary activity in the field CA1. Weakness of postexcitatory inhibition, absence of responses of the form of suppression of spontaneous activity, higher effectiveness of low-frequency stimulation of Schaffer's collaterals were observed in experiments in vitro. Posttetanic effects were more frequently observed and lasted longer in vitro than in vivo. The dominating effect in vivo was posttetanic depression and in vitro--posttetanic potentiation. The possible reasons for these differences are discussed."}
{"id": "PMID:460493", "title": "[Demonstration of the sources of several descending forebrain systems using the horseradish peroxidase retrograde axonal transport technic].", "content": "Cells of cortical and diencephalic fibres descending to the mesencephalic and bulbar reticular formation and to the dorsal column nuclei were identified. According to findings neurons sending fibres to the mesencephalic reticular formation are located in orbitofrontal pole and in area 6 of the pericruciate cortex, mainly ipsilaterally. Neurons which distribute fibres to the bulbar reticular formation are concentrated mainly in area 6 and 4 of the both hemispheres, while those distributing fibres to dorsal column nuclei are arranged in areas 1, 2, 3 and 4, mainly contralaterally. Fibres descending to reticular formation from the limbic cortex, septum, substantia innominata and amygdala were also found. The functional significance of the investigated descending pathways of forebrain is discussed.", "contents": "[Demonstration of the sources of several descending forebrain systems using the horseradish peroxidase retrograde axonal transport technic]. Cells of cortical and diencephalic fibres descending to the mesencephalic and bulbar reticular formation and to the dorsal column nuclei were identified. According to findings neurons sending fibres to the mesencephalic reticular formation are located in orbitofrontal pole and in area 6 of the pericruciate cortex, mainly ipsilaterally. Neurons which distribute fibres to the bulbar reticular formation are concentrated mainly in area 6 and 4 of the both hemispheres, while those distributing fibres to dorsal column nuclei are arranged in areas 1, 2, 3 and 4, mainly contralaterally. Fibres descending to reticular formation from the limbic cortex, septum, substantia innominata and amygdala were also found. The functional significance of the investigated descending pathways of forebrain is discussed."}
{"id": "PMID:460494", "title": "[Characteristics of the responses of cat visual cortex neurons to photic stimulation of different areas of their receptive fields].", "content": "The functions of intensity, thresholds of reactions, thresholds of inhibitory effects and differential sensitivity to intensity of local light stimulation of central and different peripheral regions of receptive fields of 96 units in the 17th field of visual cortex were investigated in immobilized and nonanesthetized cats in dark adaptation. Receptive fields of cells had wide threshold and superthreshold reliefs (3-30 degrees). Some of them had V-shape reliefs, while others--reliefs with alternating zones of high and low excitability. As a rule, sensitivity of excitatory and inhibitory inputs was maximal in the centre of the receptive field. In the investigated population of cells the inhibitory inputs were more standard in sensitivity and on the average of lower excitability. The threshold relief of the receptive fields was markedly narrowed under light adaptation mainly due to a decrease in the peripheral inputs sensitivity. The number of low-threshold units, the differential brightness sensitivity and sensitivity of the inhibitory system increased in the visual cortex as compared to the lateral geniculate and retinal units. The mechanisms of receptive field formation in the visual cortex and their plasticity depending on the adaptation level, the role of excitatory and inhibitory inputs in these effects and behavioral significance of the obtained data are discussed.", "contents": "[Characteristics of the responses of cat visual cortex neurons to photic stimulation of different areas of their receptive fields]. The functions of intensity, thresholds of reactions, thresholds of inhibitory effects and differential sensitivity to intensity of local light stimulation of central and different peripheral regions of receptive fields of 96 units in the 17th field of visual cortex were investigated in immobilized and nonanesthetized cats in dark adaptation. Receptive fields of cells had wide threshold and superthreshold reliefs (3-30 degrees). Some of them had V-shape reliefs, while others--reliefs with alternating zones of high and low excitability. As a rule, sensitivity of excitatory and inhibitory inputs was maximal in the centre of the receptive field. In the investigated population of cells the inhibitory inputs were more standard in sensitivity and on the average of lower excitability. The threshold relief of the receptive fields was markedly narrowed under light adaptation mainly due to a decrease in the peripheral inputs sensitivity. The number of low-threshold units, the differential brightness sensitivity and sensitivity of the inhibitory system increased in the visual cortex as compared to the lateral geniculate and retinal units. The mechanisms of receptive field formation in the visual cortex and their plasticity depending on the adaptation level, the role of excitatory and inhibitory inputs in these effects and behavioral significance of the obtained data are discussed."}
{"id": "PMID:460495", "title": "[Impulse activity of interneurons of the lumbar portion of the spinal cord during the late long discharges in the motor nerves of immobilized thalamic cats].", "content": "Impulse activity of lumbar interneurons was recorded extracellularly during the late long-lasting discharges in efferent nerves of immobilized thalamic animals. 70% of investigated neurons changed their activity during the late discharges. The activity of 35% of neurons increased, during late discharges in flexor nerves, while during activity in extensor nerves it was inhibited. 27% of neurons had the opposite reaction. Neurons, whose activity correspondingly increased (5%) or decreased (3%) during the late discharges both in flexor and extensor nerves were also observed. Most interneurons, changing their activity during late discharges, were located in the lateral parts of intermediate zone and in the lateral ventral horn at the depth of 2.8 mm. Certain dependence between the character of afferent inputs to the neuron and correlation of its activity with late efferent discharges was revealed. Neurons whose activity did not change during late discharges (40%) were mainly located somewhat dorsally, at the depth of about 2.0 mm. Possible role of described interneurons in generation of late discharges is discussed.", "contents": "[Impulse activity of interneurons of the lumbar portion of the spinal cord during the late long discharges in the motor nerves of immobilized thalamic cats]. Impulse activity of lumbar interneurons was recorded extracellularly during the late long-lasting discharges in efferent nerves of immobilized thalamic animals. 70% of investigated neurons changed their activity during the late discharges. The activity of 35% of neurons increased, during late discharges in flexor nerves, while during activity in extensor nerves it was inhibited. 27% of neurons had the opposite reaction. Neurons, whose activity correspondingly increased (5%) or decreased (3%) during the late discharges both in flexor and extensor nerves were also observed. Most interneurons, changing their activity during late discharges, were located in the lateral parts of intermediate zone and in the lateral ventral horn at the depth of 2.8 mm. Certain dependence between the character of afferent inputs to the neuron and correlation of its activity with late efferent discharges was revealed. Neurons whose activity did not change during late discharges (40%) were mainly located somewhat dorsally, at the depth of about 2.0 mm. Possible role of described interneurons in generation of late discharges is discussed."}
{"id": "PMID:460496", "title": "[Neurons of the superior cervical segments responding to stimulation of the bulbar locomotor strip].", "content": "Synaptic responses of single neurons of upper cervical segments to stimulation of the bulbar \"locomotor strip\" were recorded extracellularly in mesencephalic cats. The stimulating current being about 30 muA, these responses usually had 2-7 ms latencies and appeared in neurons located at a depth of 2-4 mm from the dorsal surface (Rexed's laminae V-VIII). These neurons cannot be excited antidromically either from lumbar or lower cervical segments. However antidromic responses, could be evoked by stimuli applied 3-5 mm caudally of the recording electrode. It is assumed that neurons in C2, C3 excited from the \"locomotor strip\" are elements of the cell column which is responsible for the polysynaptic propagation of activity to the spinal generators of stepping.", "contents": "[Neurons of the superior cervical segments responding to stimulation of the bulbar locomotor strip]. Synaptic responses of single neurons of upper cervical segments to stimulation of the bulbar \"locomotor strip\" were recorded extracellularly in mesencephalic cats. The stimulating current being about 30 muA, these responses usually had 2-7 ms latencies and appeared in neurons located at a depth of 2-4 mm from the dorsal surface (Rexed's laminae V-VIII). These neurons cannot be excited antidromically either from lumbar or lower cervical segments. However antidromic responses, could be evoked by stimuli applied 3-5 mm caudally of the recording electrode. It is assumed that neurons in C2, C3 excited from the \"locomotor strip\" are elements of the cell column which is responsible for the polysynaptic propagation of activity to the spinal generators of stepping."}
{"id": "PMID:460514", "title": "Brain neovascularization with temporalis muscle in dogs.", "content": "Nine mongrel adult dogs underwent temporalis muscle transposition on the surface of the left hemisphere, the arachnoid having been removed. Histological confirmation of vessel networks connecting muscle and brain was achieved after 90 days.", "contents": "Brain neovascularization with temporalis muscle in dogs. Nine mongrel adult dogs underwent temporalis muscle transposition on the surface of the left hemisphere, the arachnoid having been removed. Histological confirmation of vessel networks connecting muscle and brain was achieved after 90 days."}
{"id": "PMID:460516", "title": "Cranioplasty with pre-formed methyl methacrylate onlay plates.", "content": "The available manufactured plates for cranioplasty of large skull defects are not always satisfactory. Recently we have used preformed methyl methacrylate plates, in combination with Proplast. This plate prepared adjusted and sterilized before the operation is both cosmetically and mechanically satisfactory.", "contents": "Cranioplasty with pre-formed methyl methacrylate onlay plates. The available manufactured plates for cranioplasty of large skull defects are not always satisfactory. Recently we have used preformed methyl methacrylate plates, in combination with Proplast. This plate prepared adjusted and sterilized before the operation is both cosmetically and mechanically satisfactory."}
{"id": "PMID:460517", "title": "Developmental changes in choline acetyltransferase and glutamate decarboxylase activity in various regions of the brain of the male, female, and neonatally androgenized female rat.", "content": "In attempt to discern effects of sex hormones on the development of neurotransmitter systems in the rat brain, choline acetyltransferase (ChAT) and glutamate decarboxylase (GAD) have been measured at postnatal days 8, 12, 25, and 60 in five regions (amygdala, anterior hypothalamus, hippocampus, olfactory bulbs, and cerebral cortex) of the brains of normal male, normal female, and neonatally androgen-treated female rats. Essentially no association between sex or of neonatal \"androgenization\" on either enzymol were found. The data, however, provide new information on the relative rates of development of ChAT and GAD in five regions of the rat brain which supplement the limited information already available in the literature. ChAT activity was highest in amygdala and hypothalamus, but developed most rapidly in hippocampus and cerebral cortex. The relative activities and patterns of development of GAD activity were similar to those of ChAT.", "contents": "Developmental changes in choline acetyltransferase and glutamate decarboxylase activity in various regions of the brain of the male, female, and neonatally androgenized female rat. In attempt to discern effects of sex hormones on the development of neurotransmitter systems in the rat brain, choline acetyltransferase (ChAT) and glutamate decarboxylase (GAD) have been measured at postnatal days 8, 12, 25, and 60 in five regions (amygdala, anterior hypothalamus, hippocampus, olfactory bulbs, and cerebral cortex) of the brains of normal male, normal female, and neonatally androgen-treated female rats. Essentially no association between sex or of neonatal \"androgenization\" on either enzymol were found. The data, however, provide new information on the relative rates of development of ChAT and GAD in five regions of the rat brain which supplement the limited information already available in the literature. ChAT activity was highest in amygdala and hypothalamus, but developed most rapidly in hippocampus and cerebral cortex. The relative activities and patterns of development of GAD activity were similar to those of ChAT."}
{"id": "PMID:460518", "title": "Isolation of synaptic junction-enriched fraction from the forebrain of day-old chickens. Preparation and characterization of chick forebrain subcellular fractions.", "content": "Synaptosomal plasma membrane (SPM) and other subcellular fractions were isolated from the forebrain of 1-day-old chickens by a procedure based on that of Davis and Bloom (16) and Cotman and Taylor (13). The procedure involves the centrifugation through a discontinuous sucrose gradient of a crude synaptosomal-mitochondrial fraction which has been lysed and weighed with iodonitrotetrazolium. SPM isolated by this method contains only small amounts of lysosomal or mitochondrial membranes and is practically devoid of contaminating microsomal membranes, as estimated by enzyme marker assays. The purity of chick-brain SPM prepared by this method is compared to the purity of chick-brain fractions obtained by two other laboratories, using different methods (4, 59). The SPM were extracted with Triton X-100 and all fractions solubilized in sodium dodecyl sulfate (sds). The delipidated proteins of all fractions were subjected to SDS-polyacrylamide electrophoresis on slab gels and stained for protein. A distinct difference was observed between the patterns given by the Triton-soluble and -insoluble fractions. Electron microscopy of the synaptic junction fraction showed numerous junctional complexes.", "contents": "Isolation of synaptic junction-enriched fraction from the forebrain of day-old chickens. Preparation and characterization of chick forebrain subcellular fractions. Synaptosomal plasma membrane (SPM) and other subcellular fractions were isolated from the forebrain of 1-day-old chickens by a procedure based on that of Davis and Bloom (16) and Cotman and Taylor (13). The procedure involves the centrifugation through a discontinuous sucrose gradient of a crude synaptosomal-mitochondrial fraction which has been lysed and weighed with iodonitrotetrazolium. SPM isolated by this method contains only small amounts of lysosomal or mitochondrial membranes and is practically devoid of contaminating microsomal membranes, as estimated by enzyme marker assays. The purity of chick-brain SPM prepared by this method is compared to the purity of chick-brain fractions obtained by two other laboratories, using different methods (4, 59). The SPM were extracted with Triton X-100 and all fractions solubilized in sodium dodecyl sulfate (sds). The delipidated proteins of all fractions were subjected to SDS-polyacrylamide electrophoresis on slab gels and stained for protein. A distinct difference was observed between the patterns given by the Triton-soluble and -insoluble fractions. Electron microscopy of the synaptic junction fraction showed numerous junctional complexes."}
{"id": "PMID:460519", "title": "Brain-specific proteins in the occipital cortex of rats housed in enriched and impoverished environments.", "content": "The occipital cortex was dissected from the brain of rats housed in either enriched or impoverished environment for four weeks. In environmentally enriched rats the weight of occipital cortex was found to be increased 5.7%, compared to environmentally impoverished rats, and the amount of protein was increased 6.0%. The amount of six nervous system-specific proteins was measured by crossed immunoelectrophoresis. Synaptin increased 4.7%, D3 increased 8.3%, and D1 increased 9.6%, whereas D2 was not significantly increased. Compared to D2, D3 and D1 were still increased significantly, although all were present in synaptosomal membrane fractions. The protein S-100 was increased 3.4% and the neuronal protein 14-3-2 was increased 12.2% for the cathodal component whereas the anodal component was not increased. The results were interpreted as representing delayed development of environment-dependent neurons in the environmentally impoverished rats.", "contents": "Brain-specific proteins in the occipital cortex of rats housed in enriched and impoverished environments. The occipital cortex was dissected from the brain of rats housed in either enriched or impoverished environment for four weeks. In environmentally enriched rats the weight of occipital cortex was found to be increased 5.7%, compared to environmentally impoverished rats, and the amount of protein was increased 6.0%. The amount of six nervous system-specific proteins was measured by crossed immunoelectrophoresis. Synaptin increased 4.7%, D3 increased 8.3%, and D1 increased 9.6%, whereas D2 was not significantly increased. Compared to D2, D3 and D1 were still increased significantly, although all were present in synaptosomal membrane fractions. The protein S-100 was increased 3.4% and the neuronal protein 14-3-2 was increased 12.2% for the cathodal component whereas the anodal component was not increased. The results were interpreted as representing delayed development of environment-dependent neurons in the environmentally impoverished rats."}
{"id": "PMID:460520", "title": "Tissue and regional distribution of cysteic acid decarboxylase. A new assay method.", "content": "A sensitive and rapid assay method method for cysteic acid decarboxylase was develped which combined the selectivity of ion exchange resin (a complete retention of the substrate, cysteic acid, and exclusion of the product, taurine) with the speed of a vacuum filtration. The synthesis and purification of 35S-labeled cysteic acid were described. The validity of the assay was established by the identification of the reaction product as taurine. With this new method, the decarboxylase activity was measured in discrete regions of bovine brain. Putamen had the highest activity, 172 pmol taurine formed/min/mg protein (100%), followed by caudate nucleus, 90%; cerebral cortex, 82%; hypothalamus, 81%; cerebellar cortex, 79%; cerebellar peduncle, 59%; thalamus, 42%; brain stem, 25%; pons, 10%; and corpus callosum, 3%. The decarboxylase activity in various mouse tissues was also determined as follows: liver, 403; brain, 145; kidney, 143; spinal cord, 59; lung, 21; and spleen, 10 pmol taurine formed/min/mg. No activity could be detected in skeleton muscle and heart, suggesting a different biosynthetic pathway for taurine synthesis in these tissues. The advantages and disadvantages of the new assay method are also discussed.", "contents": "Tissue and regional distribution of cysteic acid decarboxylase. A new assay method. A sensitive and rapid assay method method for cysteic acid decarboxylase was develped which combined the selectivity of ion exchange resin (a complete retention of the substrate, cysteic acid, and exclusion of the product, taurine) with the speed of a vacuum filtration. The synthesis and purification of 35S-labeled cysteic acid were described. The validity of the assay was established by the identification of the reaction product as taurine. With this new method, the decarboxylase activity was measured in discrete regions of bovine brain. Putamen had the highest activity, 172 pmol taurine formed/min/mg protein (100%), followed by caudate nucleus, 90%; cerebral cortex, 82%; hypothalamus, 81%; cerebellar cortex, 79%; cerebellar peduncle, 59%; thalamus, 42%; brain stem, 25%; pons, 10%; and corpus callosum, 3%. The decarboxylase activity in various mouse tissues was also determined as follows: liver, 403; brain, 145; kidney, 143; spinal cord, 59; lung, 21; and spleen, 10 pmol taurine formed/min/mg. No activity could be detected in skeleton muscle and heart, suggesting a different biosynthetic pathway for taurine synthesis in these tissues. The advantages and disadvantages of the new assay method are also discussed."}
{"id": "PMID:460521", "title": "alpha-Methyl-p-tyrosine inhibits the production of free arachidonic acid and diacylglycerols in brain after a single electroconvulsive shock.", "content": "A single electroconvulsive shock (ECS) significantly enlarged the free fatty acid (FFA) pool of the mouse brain, arachidonic acid being the most actively released FFA (48% over controls). In animals pretreated with alpha-methyl-p-tyrosine (alpha-MT), the endogenous levels of FFA (25 sec after decapitation) were decreased and the effect of ECS was completely abolished. The most pronounced inhibition took place in free arachidonic acid (42% and 52% under controls in nonstimulated and ECS-stimulated mice, respectively). A similar tendency, although lower and less specific than the one taking place in FFA, was observed in mouse brain diacylglycerols (DG). In contrast to ECS, alpha-MT treatment did not effect the marked release of FFA and DG taking place 3 min after decapitation. Taking into account the specific inhibitory action of alpha-MT on tyrosine hydroxylase activity, the present findings provide experimental in vivo evidence about the relationship between biogenic amines and membrane lipid breakdown during electrical stimulation and suggest an involvement of FFA and DG in neurotransmission.", "contents": "alpha-Methyl-p-tyrosine inhibits the production of free arachidonic acid and diacylglycerols in brain after a single electroconvulsive shock. A single electroconvulsive shock (ECS) significantly enlarged the free fatty acid (FFA) pool of the mouse brain, arachidonic acid being the most actively released FFA (48% over controls). In animals pretreated with alpha-methyl-p-tyrosine (alpha-MT), the endogenous levels of FFA (25 sec after decapitation) were decreased and the effect of ECS was completely abolished. The most pronounced inhibition took place in free arachidonic acid (42% and 52% under controls in nonstimulated and ECS-stimulated mice, respectively). A similar tendency, although lower and less specific than the one taking place in FFA, was observed in mouse brain diacylglycerols (DG). In contrast to ECS, alpha-MT treatment did not effect the marked release of FFA and DG taking place 3 min after decapitation. Taking into account the specific inhibitory action of alpha-MT on tyrosine hydroxylase activity, the present findings provide experimental in vivo evidence about the relationship between biogenic amines and membrane lipid breakdown during electrical stimulation and suggest an involvement of FFA and DG in neurotransmission."}
{"id": "PMID:460522", "title": "Effect of intraventricularly injected ricin on protein synthesis in rat brain.", "content": "Ricin, a protein from the seeds of Ricinus communis which inhibits protein synthesis by eukaryotic ribosomes, is highly toxic when injected intraventricularly to rats, the LD50 being 0.241 micrograms/rat at 72 hr and 0.084 micrograms/rat at 7 days. Poisoned animals showed signs of central depression; they did not die before 20 hr of intoxication. Incorporation of amino acids in vivo into brain total protein and into brain ribosomes was impaired, as was protein synthesis in vitro by microsomes isolated from the brain of poisoned rats.", "contents": "Effect of intraventricularly injected ricin on protein synthesis in rat brain. Ricin, a protein from the seeds of Ricinus communis which inhibits protein synthesis by eukaryotic ribosomes, is highly toxic when injected intraventricularly to rats, the LD50 being 0.241 micrograms/rat at 72 hr and 0.084 micrograms/rat at 7 days. Poisoned animals showed signs of central depression; they did not die before 20 hr of intoxication. Incorporation of amino acids in vivo into brain total protein and into brain ribosomes was impaired, as was protein synthesis in vitro by microsomes isolated from the brain of poisoned rats."}
{"id": "PMID:460523", "title": "The synthesis and turnover of spermidine and spermine in mouse brain.", "content": "Following the administration to mice of radiolabeled putrescine by intraventricular injection, changes in the specific radioactivity of putrescine, spermidine, and spermine have been measured. Putrescine decline was biphasic, being more rapid over the first 12 hr(t 1/2 = 5 hr) than over the remainder of the 48-hr period (t 1/2 = 11 hr) that significant labeling was detected. Spermidine was rapidly labeled during the decline in putrescine radioactivity and maximum incorporation of label occurred at 18 hr. Subsequently, spermidine specific activity declined with a half-life of 22 days. Spermine synthesis was slower, with maximum labeling occurring after 4 days. Spermine turnover, measured at a time when spermidine radioactivity had substantially declined, was extremely slow (t 1/2 = 92 days). The data supports the view that putrescine is a precursor of spermidine which in turn is required for spermine synthesis.", "contents": "The synthesis and turnover of spermidine and spermine in mouse brain. Following the administration to mice of radiolabeled putrescine by intraventricular injection, changes in the specific radioactivity of putrescine, spermidine, and spermine have been measured. Putrescine decline was biphasic, being more rapid over the first 12 hr(t 1/2 = 5 hr) than over the remainder of the 48-hr period (t 1/2 = 11 hr) that significant labeling was detected. Spermidine was rapidly labeled during the decline in putrescine radioactivity and maximum incorporation of label occurred at 18 hr. Subsequently, spermidine specific activity declined with a half-life of 22 days. Spermine synthesis was slower, with maximum labeling occurring after 4 days. Spermine turnover, measured at a time when spermidine radioactivity had substantially declined, was extremely slow (t 1/2 = 92 days). The data supports the view that putrescine is a precursor of spermidine which in turn is required for spermine synthesis."}
{"id": "PMID:460525", "title": "Reciprocal changes in center and surrounding S potentials of fish retina in response to dopamine.", "content": "Effects of dopamine (DA) were examined on the intracellularly recorded potential from horizontal cells in the fish (Eugerres plumieri) rentina. DA (100 micron in the perfusate) augmented the center S potential in a response to a spot illumination and attenuated the surrounding S potential to an annular light by approximately 40%. These reciprocal changes in the S potentials were associated with a slight depolarization (2.5 mV) of the horizontal cell, and were reversible in 10-15 min. The results indicate that DA at this concentration does not affect directly the synaptic transmission from photoreceptors to horizontal cells, while it appears to interfere selectively with the lateral propagation of an S potential. The effects of DA observed may represent an aspect of function of DA-containing interplexiform cells in the retina.", "contents": "Reciprocal changes in center and surrounding S potentials of fish retina in response to dopamine. Effects of dopamine (DA) were examined on the intracellularly recorded potential from horizontal cells in the fish (Eugerres plumieri) rentina. DA (100 micron in the perfusate) augmented the center S potential in a response to a spot illumination and attenuated the surrounding S potential to an annular light by approximately 40%. These reciprocal changes in the S potentials were associated with a slight depolarization (2.5 mV) of the horizontal cell, and were reversible in 10-15 min. The results indicate that DA at this concentration does not affect directly the synaptic transmission from photoreceptors to horizontal cells, while it appears to interfere selectively with the lateral propagation of an S potential. The effects of DA observed may represent an aspect of function of DA-containing interplexiform cells in the retina."}
{"id": "PMID:460526", "title": "Glycoprotein galactosyltransferase activity in synaptic junctional complexes isolated from rat forebrain.", "content": "A synaptosomal plasma membrane fraction and its junctional and nonjunctional subfractions were isolated and analyzed for glycoprotein galactosyltransferase activity. The nonjunctional components fraction had the highest specific activity in the presence of exogenous acceptor, suggesting an enrichment of enzyme in this fraction. The synaptic junctional complex fraction had the highest specific activity in the absence of added acceptor, suggesting that there is a relative enrichment of endogenous acceptors for this galactosyltransferase within the synaptic junctional complex.", "contents": "Glycoprotein galactosyltransferase activity in synaptic junctional complexes isolated from rat forebrain. A synaptosomal plasma membrane fraction and its junctional and nonjunctional subfractions were isolated and analyzed for glycoprotein galactosyltransferase activity. The nonjunctional components fraction had the highest specific activity in the presence of exogenous acceptor, suggesting an enrichment of enzyme in this fraction. The synaptic junctional complex fraction had the highest specific activity in the absence of added acceptor, suggesting that there is a relative enrichment of endogenous acceptors for this galactosyltransferase within the synaptic junctional complex."}
{"id": "PMID:460527", "title": "Transport of malate and citrate into rat brain mitochondria under hypoxia and anesthesia.", "content": "Hypoxia and anesthesia inhibited penetration of malate and citrate into the brain mitochondria by 60% and 40%, respectively. Anesthetized animals exposed to low oxygen tension showed similar changes as those subjected to hypoxia without anesthesia. Recovery from anesthesia was rapid and the rates of citrate and malate uptake returned to the control values in approximately 60 min. In the case of hypoxia, days were required to restore the control values of citrate and malate uptake. Free fatty acids had no effect on the entry of malate and citrate into the mitochondria. However, changes in the levels of protein --SH groups were observed which may be responsible for the impaired transport of citrate and malate under hypoxic condition.", "contents": "Transport of malate and citrate into rat brain mitochondria under hypoxia and anesthesia. Hypoxia and anesthesia inhibited penetration of malate and citrate into the brain mitochondria by 60% and 40%, respectively. Anesthetized animals exposed to low oxygen tension showed similar changes as those subjected to hypoxia without anesthesia. Recovery from anesthesia was rapid and the rates of citrate and malate uptake returned to the control values in approximately 60 min. In the case of hypoxia, days were required to restore the control values of citrate and malate uptake. Free fatty acids had no effect on the entry of malate and citrate into the mitochondria. However, changes in the levels of protein --SH groups were observed which may be responsible for the impaired transport of citrate and malate under hypoxic condition."}
{"id": "PMID:460528", "title": "Comparison of high-affinity binding of [3H]GABA to subcellular particles of rat brain and liver.", "content": "The binding of [3H]GABA and retention of [14C]sucrose have been studied in freshly prepared \"synaptosomal-mitochondrial\" (P2) fractions of rat cerebral cortex and liver using bicarbonate-buffered medium (containing 147 mEq/liter of Na+), and in frozen/thawed crude membrane fractions of rat whole brain and liver using Na+-free Tris HCl medium. GABA-sensitive sites (GSS) and bicuculline-methiodide- (BMI-) sensitive sites (BMI-SS) were defined as those amounts of [3H]GABA that were sensitive to the displacement by 10(-3) M unlabeled GABA or BMI. In the presence of added Na+, two high-affinity GABA-binding processes were detected in the P2 fraction of cerebral cortex. The lower-affinity process (likely related mainly to uptake sites) had KB approximately equal to 10(-5) M, Bmax for GSS approximately equal to 3 nmol/mg protein, and Bmax for BMI-SS approximately equal to 0.5 nmol/mg protein, whereas the higher-affinity process (likely related to synaptic GABA receptors) had KB approximately equal to 10(-7) M, BMAX for GSS approximately equal to 43 pmol/mg protein, and BMAX for BMI-SS approximately equal to 2 pmol/mg proteins. Only the higher-affinity process was detected in the liver P2 fraction, and it had KB approximately equal to 3.7 x 10(-8) M, BMAX for GSS approximately equal to 0.48 pmol/mg protein, and BMAX for BMI-SS approximately equal to 0.1 pmol/mg protein (i.e., about 1/100 and 1/20 the receptive BMAX values of cerebral cortex). This binding process of the liver P2 fraction could represent sites involved in mitochondrial GABA transport. In Na+-free Tris HCl medium, high-affinity [3H]GABA binding appeared to exist in frozen/thawed membrane preparations of both brain and liver when data were expressed on a protein basis. However, this binding to liver membranes was not displaceable by 10(-5) M unlabeled GABA, and when these data were expressed on a weight basis and corrected for [3H]GABA present in trapped supernatant fluid of the pellets, no [3H]GABA binding was detected in the liver preparation.", "contents": "Comparison of high-affinity binding of [3H]GABA to subcellular particles of rat brain and liver. The binding of [3H]GABA and retention of [14C]sucrose have been studied in freshly prepared \"synaptosomal-mitochondrial\" (P2) fractions of rat cerebral cortex and liver using bicarbonate-buffered medium (containing 147 mEq/liter of Na+), and in frozen/thawed crude membrane fractions of rat whole brain and liver using Na+-free Tris HCl medium. GABA-sensitive sites (GSS) and bicuculline-methiodide- (BMI-) sensitive sites (BMI-SS) were defined as those amounts of [3H]GABA that were sensitive to the displacement by 10(-3) M unlabeled GABA or BMI. In the presence of added Na+, two high-affinity GABA-binding processes were detected in the P2 fraction of cerebral cortex. The lower-affinity process (likely related mainly to uptake sites) had KB approximately equal to 10(-5) M, Bmax for GSS approximately equal to 3 nmol/mg protein, and Bmax for BMI-SS approximately equal to 0.5 nmol/mg protein, whereas the higher-affinity process (likely related to synaptic GABA receptors) had KB approximately equal to 10(-7) M, BMAX for GSS approximately equal to 43 pmol/mg protein, and BMAX for BMI-SS approximately equal to 2 pmol/mg proteins. Only the higher-affinity process was detected in the liver P2 fraction, and it had KB approximately equal to 3.7 x 10(-8) M, BMAX for GSS approximately equal to 0.48 pmol/mg protein, and BMAX for BMI-SS approximately equal to 0.1 pmol/mg protein (i.e., about 1/100 and 1/20 the receptive BMAX values of cerebral cortex). This binding process of the liver P2 fraction could represent sites involved in mitochondrial GABA transport. In Na+-free Tris HCl medium, high-affinity [3H]GABA binding appeared to exist in frozen/thawed membrane preparations of both brain and liver when data were expressed on a protein basis. However, this binding to liver membranes was not displaceable by 10(-5) M unlabeled GABA, and when these data were expressed on a weight basis and corrected for [3H]GABA present in trapped supernatant fluid of the pellets, no [3H]GABA binding was detected in the liver preparation."}
{"id": "PMID:460529", "title": "Transport of tryptophan and tyrosine in rat brain slices in the presence of lithium.", "content": "Slices from rat cerebral cortex, brain stem, and cerebellum were incubated in media in which 1, 10, or 100 mmol/liter NaC1 had been replaced by equimolar amounts of LiC1. The initial influx of tryptophan and tyrosine into the slices diminished in the lithium-containing media. The lithium-induced inhibition was not competitive. The equilibrium accumulation of the amino acids was also less in the presence of LiC1. The incorporation of tryptophan and tyrosine into the proteins of the slices was inhibited by lithium. These were no clear differences between the brain areas studied. It has been suggested earlier that a lithium treatment enhances the in vivo cerebral uptake of these aromatic amino acids. The present results show that such a possible increase in uptake is not a direct effect of lithium ions on cell membranes.", "contents": "Transport of tryptophan and tyrosine in rat brain slices in the presence of lithium. Slices from rat cerebral cortex, brain stem, and cerebellum were incubated in media in which 1, 10, or 100 mmol/liter NaC1 had been replaced by equimolar amounts of LiC1. The initial influx of tryptophan and tyrosine into the slices diminished in the lithium-containing media. The lithium-induced inhibition was not competitive. The equilibrium accumulation of the amino acids was also less in the presence of LiC1. The incorporation of tryptophan and tyrosine into the proteins of the slices was inhibited by lithium. These were no clear differences between the brain areas studied. It has been suggested earlier that a lithium treatment enhances the in vivo cerebral uptake of these aromatic amino acids. The present results show that such a possible increase in uptake is not a direct effect of lithium ions on cell membranes."}
{"id": "PMID:460530", "title": "[A case of nasofrontal encephalomeningocele with review of literatures and experiences of 14 cases of cephaloceles (author's transl)].", "content": "A case of 2-month-old boy with nasofrontal encephalomeningocele was presented, which was excellently treated via intradural approach, followed by removal of herniated sac. Under the knowledge of reviewed literatures and our experiences of 14 cases of cephaloceles, its symptomatology, diagnosis and policy of treatments were discussed. As rare case was an intracranial meningoencephalocele in our series described, which showed a supracollicular cyst with brain tissue wall, communicating with aqueduct. Other emphases were placed on the differential diagnosis from holoprosencephaly with extracranial dorsal sac, the usefulness of CT-scan as a diagnostic tool and our method of closing dural defects in anterior cranial fossa by reflecting dura on frontal lobe.", "contents": "[A case of nasofrontal encephalomeningocele with review of literatures and experiences of 14 cases of cephaloceles (author's transl)]. A case of 2-month-old boy with nasofrontal encephalomeningocele was presented, which was excellently treated via intradural approach, followed by removal of herniated sac. Under the knowledge of reviewed literatures and our experiences of 14 cases of cephaloceles, its symptomatology, diagnosis and policy of treatments were discussed. As rare case was an intracranial meningoencephalocele in our series described, which showed a supracollicular cyst with brain tissue wall, communicating with aqueduct. Other emphases were placed on the differential diagnosis from holoprosencephaly with extracranial dorsal sac, the usefulness of CT-scan as a diagnostic tool and our method of closing dural defects in anterior cranial fossa by reflecting dura on frontal lobe."}
{"id": "PMID:460535", "title": "[Silicone pellet embolization of spinal cord arteriovenous malformation--case report (author's transl)].", "content": "This is a report of case with the spinal cord arteriovenous malformation in which silicone pellet embolizations of the feeding arteries were carried out through a percutaneous catheter. The technical details are presented. (Case report) A 35-year-old paraplegic patient. Her radiograms are shown Figs. 1 to 5. An attempt to direct removal of the AVM was limited to the laminectomy (Th1--4) because of extensive haemorrhage in the extradural space. Small silicon pellets with barium sulfate were used to occlude the feeder in this case. The large AVM at the high thoracic level was supplied by feeders coming from the branches of thyreocervical trunk in both side. In the right, six pellets of a proper size were used. Angiogram taken immediately after injection of emboli, demonstrated a decrease of volume of the AVM to about a half (Fig. 2-b), and six months later, the spinal feeders had been embolized almost completely (Fig. 5-a). In the left side, two pellets were injected through the left thyreocervical trunk but the malformation was depicted at the follow-up angiogram taken about six months later. She went to the rehabilitation clinic after surgery and her palaplegia improved remarkably. At present, she can walk on crutches. Disturbances to evacuation and urination have also disappeared.", "contents": "[Silicone pellet embolization of spinal cord arteriovenous malformation--case report (author's transl)]. This is a report of case with the spinal cord arteriovenous malformation in which silicone pellet embolizations of the feeding arteries were carried out through a percutaneous catheter. The technical details are presented. (Case report) A 35-year-old paraplegic patient. Her radiograms are shown Figs. 1 to 5. An attempt to direct removal of the AVM was limited to the laminectomy (Th1--4) because of extensive haemorrhage in the extradural space. Small silicon pellets with barium sulfate were used to occlude the feeder in this case. The large AVM at the high thoracic level was supplied by feeders coming from the branches of thyreocervical trunk in both side. In the right, six pellets of a proper size were used. Angiogram taken immediately after injection of emboli, demonstrated a decrease of volume of the AVM to about a half (Fig. 2-b), and six months later, the spinal feeders had been embolized almost completely (Fig. 5-a). In the left side, two pellets were injected through the left thyreocervical trunk but the malformation was depicted at the follow-up angiogram taken about six months later. She went to the rehabilitation clinic after surgery and her palaplegia improved remarkably. At present, she can walk on crutches. Disturbances to evacuation and urination have also disappeared."}
{"id": "PMID:460537", "title": "[Posterior subtemporal transtentorial approach for a lower basilar trunk aneurysm (author's transl)].", "content": "A lower basilar trunk aneurysm is rare and it has been difficult to operate on this kind of aneurysm which is located in so-called \"no man's land\". We have recently operated on the aneurysm which was located between the vertebrobasilar junction and the origin of AICA. The aneurysm was approached posterior-subtemporal-transtentorially and wrapped with a muscle piece because of its broad neck. After the operation the patient developed amnestic aphasia which, however, disappeared 4 months postoperatively. The advantage of this approach is that it enables a better visualization of the lower basilar trunk, the lateroventral portion of the pons, the distal part (5 mm) of both vertebral arteries and the upper portion of the medulla oblongata than any other approaches hitherto reported. The retraction of the temporal lobe and subsequent brain damage may be minimized by using intraoperative ventricular drainage and microtechnical maneuver.", "contents": "[Posterior subtemporal transtentorial approach for a lower basilar trunk aneurysm (author's transl)]. A lower basilar trunk aneurysm is rare and it has been difficult to operate on this kind of aneurysm which is located in so-called \"no man's land\". We have recently operated on the aneurysm which was located between the vertebrobasilar junction and the origin of AICA. The aneurysm was approached posterior-subtemporal-transtentorially and wrapped with a muscle piece because of its broad neck. After the operation the patient developed amnestic aphasia which, however, disappeared 4 months postoperatively. The advantage of this approach is that it enables a better visualization of the lower basilar trunk, the lateroventral portion of the pons, the distal part (5 mm) of both vertebral arteries and the upper portion of the medulla oblongata than any other approaches hitherto reported. The retraction of the temporal lobe and subsequent brain damage may be minimized by using intraoperative ventricular drainage and microtechnical maneuver."}
{"id": "PMID:460538", "title": "Effects of bromocriptine on plasma catecholamines in normal men.", "content": "Administration of a single oral 2.5 mg dose of bromocriptine (Brc), a dopamine (DA) receptor agonist, to normal male volunteers results in comparable marked decreases in resting supine plasma concentrations of DA (decrement 2 h after drug administration was 67 +/- 5%; mean +/- SE), norepinephrine (NE; 63 +/- 3%) and epinephrine (E; 65 +/- 5%); decreases in all three plasma catecholamine (CA) concentrations were significant at p less than 0.001. This effect of Brc on plasma CAs was initiated more rapidly and was of briefer duration than the suppressive effect on serum prolactin (Prl). When Brc was administered daily for 1 week, its effect on plasma CAs had terminated within 4 h of its adninistration. A further oral dose after administration of Brc for 1 week produced identical suppressive effects on plasma CAs to those observed following the 1st dose. Brc did not prevent the incremental response of plasma DA, NE and E to standing. Also, orthostatic hypotension occurred on occasion, in spite of normal plasma CA responses to standing. Brc did not affect basal serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone or dihydrotestosterone. These data describing a suppressive effect of Brc on plasma DA, NE and E suggest that Brc inhibits release of these CAs from peripheral sympathetic nerve endings and adrenal medulla, and acts at least in part through presynaptic CA receptors in the brain. The suppressive effect of Brc on plasma CAs may prove useful as a test of normal presynaptic CA mechanisms.", "contents": "Effects of bromocriptine on plasma catecholamines in normal men. Administration of a single oral 2.5 mg dose of bromocriptine (Brc), a dopamine (DA) receptor agonist, to normal male volunteers results in comparable marked decreases in resting supine plasma concentrations of DA (decrement 2 h after drug administration was 67 +/- 5%; mean +/- SE), norepinephrine (NE; 63 +/- 3%) and epinephrine (E; 65 +/- 5%); decreases in all three plasma catecholamine (CA) concentrations were significant at p less than 0.001. This effect of Brc on plasma CAs was initiated more rapidly and was of briefer duration than the suppressive effect on serum prolactin (Prl). When Brc was administered daily for 1 week, its effect on plasma CAs had terminated within 4 h of its adninistration. A further oral dose after administration of Brc for 1 week produced identical suppressive effects on plasma CAs to those observed following the 1st dose. Brc did not prevent the incremental response of plasma DA, NE and E to standing. Also, orthostatic hypotension occurred on occasion, in spite of normal plasma CA responses to standing. Brc did not affect basal serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone or dihydrotestosterone. These data describing a suppressive effect of Brc on plasma DA, NE and E suggest that Brc inhibits release of these CAs from peripheral sympathetic nerve endings and adrenal medulla, and acts at least in part through presynaptic CA receptors in the brain. The suppressive effect of Brc on plasma CAs may prove useful as a test of normal presynaptic CA mechanisms."}
{"id": "PMID:460539", "title": "LH release and ovulation in the rat following depletion of hypothalamic norepinephrine: chronic vs. acute effects.", "content": "The effects of long-term hypothalamic norepinephrine (NE) depletion on reproductive cyclicity, ovulation, and luteinizing hormone (LH) release were studied in adult female rats. Bilateral transections of the ascending noradrenergic pathway (ANP) at the level of the mesencephalon led to an 83% depletion of hypothalamic NE. Although some animals exhibited a period of acyclicity following this surgical procedure, by 23 days all animals had begun to cycle. When checked for ovulation at around 40 days, all of the rats had shed full quotas of ova. There was no significant difference in either the timing or magnitude of the surge when proestrous LH levels in NE-depleted rats were compared with sham transected controls. To see if the residual hypothalamic NE left after transection of the ascending noradrenergic pathway was important for maintenance of normal LH secretion and ovulation, a group of long-term depleted rats were given an NE synthesis inhibitor (diethyldithiocarbamate: DDC). In all of the sham-cut and intact control rats this drug either partially or completely blocked ovulation, but it was effective in only 40% of the depleted animals. The other 60% not only ovulated, but shed full quotas of ova. These results demonstrate that normal LH secretion can occur even when hyplthalamic NE levels are extremely low. Although acute depressions of NE by pharmacological agents such as DDC effectively block the LH surge, this inhibition is not sustained when NE is depleted over long periods of time. Therefore, NE apparently plays a modulatory, rather than mandatory, role in control of LH release and ovulation.", "contents": "LH release and ovulation in the rat following depletion of hypothalamic norepinephrine: chronic vs. acute effects. The effects of long-term hypothalamic norepinephrine (NE) depletion on reproductive cyclicity, ovulation, and luteinizing hormone (LH) release were studied in adult female rats. Bilateral transections of the ascending noradrenergic pathway (ANP) at the level of the mesencephalon led to an 83% depletion of hypothalamic NE. Although some animals exhibited a period of acyclicity following this surgical procedure, by 23 days all animals had begun to cycle. When checked for ovulation at around 40 days, all of the rats had shed full quotas of ova. There was no significant difference in either the timing or magnitude of the surge when proestrous LH levels in NE-depleted rats were compared with sham transected controls. To see if the residual hypothalamic NE left after transection of the ascending noradrenergic pathway was important for maintenance of normal LH secretion and ovulation, a group of long-term depleted rats were given an NE synthesis inhibitor (diethyldithiocarbamate: DDC). In all of the sham-cut and intact control rats this drug either partially or completely blocked ovulation, but it was effective in only 40% of the depleted animals. The other 60% not only ovulated, but shed full quotas of ova. These results demonstrate that normal LH secretion can occur even when hyplthalamic NE levels are extremely low. Although acute depressions of NE by pharmacological agents such as DDC effectively block the LH surge, this inhibition is not sustained when NE is depleted over long periods of time. Therefore, NE apparently plays a modulatory, rather than mandatory, role in control of LH release and ovulation."}
{"id": "PMID:460549", "title": "Head trauma and subsequent brain tumors.", "content": "A long term study of 2,953 persons with 29,859 person-years of follow-up after head injury provided an opportunity to investigate the long-debated association between head trauma and subsequent intracranial tumor. In this series the observed number of cases of subsequent brain tumor did not differ from the number we expected. Because brain tumors are relatively rare, the results of this large series cannot absolutely refute the possibility that head trauma predisposes to brain tumor, but the individual risk is very small, and the weight of evidence does not support an etiological association. The occurrence of subsequent brain tumors in this series was not associated with the severity or location of the head injury. Head trauma does not seem to be a significant etiological factor in meningioma; that tumor has a higher incidence in females, whereas males have 2- to 3-fold greater incidence of head trauma. If any association between head trauma and subsequent brain tumor does exist, it is extremely small or occurs only in the presence of other factors, which themselves must be rare.", "contents": "Head trauma and subsequent brain tumors. A long term study of 2,953 persons with 29,859 person-years of follow-up after head injury provided an opportunity to investigate the long-debated association between head trauma and subsequent intracranial tumor. In this series the observed number of cases of subsequent brain tumor did not differ from the number we expected. Because brain tumors are relatively rare, the results of this large series cannot absolutely refute the possibility that head trauma predisposes to brain tumor, but the individual risk is very small, and the weight of evidence does not support an etiological association. The occurrence of subsequent brain tumors in this series was not associated with the severity or location of the head injury. Head trauma does not seem to be a significant etiological factor in meningioma; that tumor has a higher incidence in females, whereas males have 2- to 3-fold greater incidence of head trauma. If any association between head trauma and subsequent brain tumor does exist, it is extremely small or occurs only in the presence of other factors, which themselves must be rare."}
{"id": "PMID:460551", "title": "Cyanoacrylate occlusion of carotid-cavernous fistula with preservation of carotid artery flow.", "content": "We report a new treatment for carotid-cavernous fistula. Using a flow-guided, balloon-tipped microcatheter, we catheterize the fistula itself, verify balloon entry into the fistula with fluoroscopy and x-ray films, and then infuse the tissue adhesive isobutyl-2-cyanoacrylate with careful fluoroscopic control. Three patients have had their fistulas occluded, with preservation of flow through the internal carotid artery. This balloon microcatheter allows the radiologist to perform a reversible test occlusion. All three patients had neurological changes during or after the procedure, and in one we inadvertently occluded several distal middle cerebral artery branches without permanent neurological deficit. No patient became blind or developed 3rd, 4th, or 6th nerve palsy from the treatment. This technique seems to have promise as another method for the obliteration of carotid-cavernous fistula.", "contents": "Cyanoacrylate occlusion of carotid-cavernous fistula with preservation of carotid artery flow. We report a new treatment for carotid-cavernous fistula. Using a flow-guided, balloon-tipped microcatheter, we catheterize the fistula itself, verify balloon entry into the fistula with fluoroscopy and x-ray films, and then infuse the tissue adhesive isobutyl-2-cyanoacrylate with careful fluoroscopic control. Three patients have had their fistulas occluded, with preservation of flow through the internal carotid artery. This balloon microcatheter allows the radiologist to perform a reversible test occlusion. All three patients had neurological changes during or after the procedure, and in one we inadvertently occluded several distal middle cerebral artery branches without permanent neurological deficit. No patient became blind or developed 3rd, 4th, or 6th nerve palsy from the treatment. This technique seems to have promise as another method for the obliteration of carotid-cavernous fistula."}
{"id": "PMID:460555", "title": "Toxic substances in spinal cord injury.", "content": "Several investigators have implicated norepinephrine and other toxic substances released in the region of a spinal cord injury in the genesis of the progressive pathological and clinical changes that follow spinal trauma. To test this hypothesis, we subjected cats to T-10 to T-12 laminectomy and monitored epidural spinal evoked potentials from sciatic nerve stimulation. The spinal subarachnoid space was perfused with normal saline, with norepinephrine solution, or with heparinized autologous blood or the pial surface of the spinal cord was exposed to macerated gray matter taken from the upper cervical cord. During 1- to 2-hour exposure periods, we noted no significant changes in the base line spinal evoked potentials. In another series of cats, we have shown that norepinephrine perfused over the spinal cord in this manner diffuses rapidly into the subpial white matter. Therefore, its failure to affect spinal evoked potentials does not represent a failure to penetrate the spinal cord. Putative toxins must originate either in extravasated blood or damaged neural tissue in the region of the spinal cord injury. The failure of ascending spinal tracts to react to blood or cord tissue in our experiment suggests that toxins are not involved in the spinal cord dysfunction that occurs soon after injury.", "contents": "Toxic substances in spinal cord injury. Several investigators have implicated norepinephrine and other toxic substances released in the region of a spinal cord injury in the genesis of the progressive pathological and clinical changes that follow spinal trauma. To test this hypothesis, we subjected cats to T-10 to T-12 laminectomy and monitored epidural spinal evoked potentials from sciatic nerve stimulation. The spinal subarachnoid space was perfused with normal saline, with norepinephrine solution, or with heparinized autologous blood or the pial surface of the spinal cord was exposed to macerated gray matter taken from the upper cervical cord. During 1- to 2-hour exposure periods, we noted no significant changes in the base line spinal evoked potentials. In another series of cats, we have shown that norepinephrine perfused over the spinal cord in this manner diffuses rapidly into the subpial white matter. Therefore, its failure to affect spinal evoked potentials does not represent a failure to penetrate the spinal cord. Putative toxins must originate either in extravasated blood or damaged neural tissue in the region of the spinal cord injury. The failure of ascending spinal tracts to react to blood or cord tissue in our experiment suggests that toxins are not involved in the spinal cord dysfunction that occurs soon after injury."}
{"id": "PMID:460564", "title": "Computerized tomographic scan and gunshot wounds of the head: indications and radiographic findings.", "content": "The authors document their experience with the computerized tomographic (CT) scanner for evaluating gunshot wounds of the head. Only those patients who were considered to be operative candidates and who were neurologically stable were scanned. In the postoperative period, patients who were not scanned preoperatively and those whose condition did not improve were also scanned. In the preoperative period the CT scanner is useful for identifying and localizing missile tracks, bony and metallic fragments, intra- and extraparenchymal hematomas, intracranial air, and brain swelling. In the postoperative period the CT scanner may demonstrate retained bone fragments, edema, brain abscess, and intracranial air. The limitations of the CT scan in evaluating gunshot wounds include an inability to define vascular lesions suchas traumatic aneurysms and post-traumatic spasm. Metallic scatter from missile fragments may render certain CT cuts uninterpretable. In addition, the CT scan may appear minimally abnormal in spite of immediate and irreversible injury caused by a shock wave transmitted to brain stem structures. The CT scanner has superceded angiography as a diagnostic tool for evaluating gunshot wounds of the head because it is noninvasive and rapid, allows visualization of the entire head, can resolve very small lesions that produce little or no mass effect, can help to determine the nature of intracranial lesions and may quantitate the amount of hemorrhage and edema. Because it enables physicians to visualize intracranial structures in three dimensions, the CT scan may precisely define missile tracks and contiguous lesions in a manner not heretofore possible with other diagnostic modalities. Thus, it is invaluable for the rational planning of surgical therapy.", "contents": "Computerized tomographic scan and gunshot wounds of the head: indications and radiographic findings. The authors document their experience with the computerized tomographic (CT) scanner for evaluating gunshot wounds of the head. Only those patients who were considered to be operative candidates and who were neurologically stable were scanned. In the postoperative period, patients who were not scanned preoperatively and those whose condition did not improve were also scanned. In the preoperative period the CT scanner is useful for identifying and localizing missile tracks, bony and metallic fragments, intra- and extraparenchymal hematomas, intracranial air, and brain swelling. In the postoperative period the CT scanner may demonstrate retained bone fragments, edema, brain abscess, and intracranial air. The limitations of the CT scan in evaluating gunshot wounds include an inability to define vascular lesions suchas traumatic aneurysms and post-traumatic spasm. Metallic scatter from missile fragments may render certain CT cuts uninterpretable. In addition, the CT scan may appear minimally abnormal in spite of immediate and irreversible injury caused by a shock wave transmitted to brain stem structures. The CT scanner has superceded angiography as a diagnostic tool for evaluating gunshot wounds of the head because it is noninvasive and rapid, allows visualization of the entire head, can resolve very small lesions that produce little or no mass effect, can help to determine the nature of intracranial lesions and may quantitate the amount of hemorrhage and edema. Because it enables physicians to visualize intracranial structures in three dimensions, the CT scan may precisely define missile tracks and contiguous lesions in a manner not heretofore possible with other diagnostic modalities. Thus, it is invaluable for the rational planning of surgical therapy."}
{"id": "PMID:460566", "title": "Ventricle to sagittal sinus shunt for hydrocephalus.", "content": "A technique of shunting the lateral ventricle to the superior sagittal sinus is described. It has been used in 36 adult patients; infection occured in 2 patients (5.6%), and 2 patients required shunt revision (5.6%). The outcome of 18 patients with high pressure hydrocephalus could be validly evaluated, and successful control was achieved in 15 (83%). One of the 3 failures could have been avoided because shunting was undertaken before the ventricular fluid had cleared of blood. Four patients with normal or low pressure hydrocephalus were treated; only 1 had a successful result. This technique is suitable for patients with high pressure hydrocephalus and is of particular value in very ill or debilitated patients because of the rapidity with which it can be performed under local analgesia. Its use in normal or low pressure hydrocephalus must still be evaluated. In any event, the sagittal sinus is certainly a feasible alternate receptor site for ventricular shunting.", "contents": "Ventricle to sagittal sinus shunt for hydrocephalus. A technique of shunting the lateral ventricle to the superior sagittal sinus is described. It has been used in 36 adult patients; infection occured in 2 patients (5.6%), and 2 patients required shunt revision (5.6%). The outcome of 18 patients with high pressure hydrocephalus could be validly evaluated, and successful control was achieved in 15 (83%). One of the 3 failures could have been avoided because shunting was undertaken before the ventricular fluid had cleared of blood. Four patients with normal or low pressure hydrocephalus were treated; only 1 had a successful result. This technique is suitable for patients with high pressure hydrocephalus and is of particular value in very ill or debilitated patients because of the rapidity with which it can be performed under local analgesia. Its use in normal or low pressure hydrocephalus must still be evaluated. In any event, the sagittal sinus is certainly a feasible alternate receptor site for ventricular shunting."}
{"id": "PMID:460567", "title": "Results of surgical lysis of lumbar adhesive arachnoiditis.", "content": "From a series of 681 patients with lumbar disc disease treated between 1966 and 1978, 17 patients required surgical lysis of lumbar adhesive arachnoiditis, 8 having initially been operated upon by another surgeon. All patients had severe pain as a predominant feature, with pain being bilateral in 9 patients. Pain was the only major symptom in 3; the other 14 exhibited varying combinations of progressive neurological dysfunction. Three patients developed late symptoms after trauma, 8 to 21 years after back surgery. At operation, multisegmental arachnoiditis was found in 5 patients and anular or subtotal adhesions were found in 12. Complete lysis could not be obtained in 4 patients. Fourteen patients were treated with steroids at the time of operation. Follow-up after lysis was less than 1 year for 5 patients but averaged 4.8 years for the remaining 12. During the 1st year after operation, 76% experienced improvement in pain (35%, good to excellent), 71% experienced improvement in neurological status. Follow-up after at least 1 year revealed 50% still enjoying pain relief (25%, good to excellent) and 45% experiencing neurological improvement. Pain relief persisted in 4 of 5 patients followed 5 years or more. The etiological role of myelograpy and lumbar disc surgery in arachnoiditis has probably been over-rated. Arachnoiditis may be symptomatic or asymptomatic and may mask other, treatable lumbar lesions. More frequent intradural exploration for discrepancies between operative and myelographic findings might reveal, and benefit, more cases of spontaneous arachnoiditis mimicking lumbar disc disease.", "contents": "Results of surgical lysis of lumbar adhesive arachnoiditis. From a series of 681 patients with lumbar disc disease treated between 1966 and 1978, 17 patients required surgical lysis of lumbar adhesive arachnoiditis, 8 having initially been operated upon by another surgeon. All patients had severe pain as a predominant feature, with pain being bilateral in 9 patients. Pain was the only major symptom in 3; the other 14 exhibited varying combinations of progressive neurological dysfunction. Three patients developed late symptoms after trauma, 8 to 21 years after back surgery. At operation, multisegmental arachnoiditis was found in 5 patients and anular or subtotal adhesions were found in 12. Complete lysis could not be obtained in 4 patients. Fourteen patients were treated with steroids at the time of operation. Follow-up after lysis was less than 1 year for 5 patients but averaged 4.8 years for the remaining 12. During the 1st year after operation, 76% experienced improvement in pain (35%, good to excellent), 71% experienced improvement in neurological status. Follow-up after at least 1 year revealed 50% still enjoying pain relief (25%, good to excellent) and 45% experiencing neurological improvement. Pain relief persisted in 4 of 5 patients followed 5 years or more. The etiological role of myelograpy and lumbar disc surgery in arachnoiditis has probably been over-rated. Arachnoiditis may be symptomatic or asymptomatic and may mask other, treatable lumbar lesions. More frequent intradural exploration for discrepancies between operative and myelographic findings might reveal, and benefit, more cases of spontaneous arachnoiditis mimicking lumbar disc disease."}
{"id": "PMID:460568", "title": "Brain hamartomas and tumors associated with tuberous sclerosis.", "content": "Ventricular tumors of tuberous sclerosis are almost always hamartomas or benign tumors occurring predominantly in children or young adults. No clear gross or histological distinctions between the ventricular \"candle gutterings\" and \"tumors\" have been identified. The clinical presentation may be acute, with the sudden onset of increased intracranial pressure and possible serious morbidity (visual impairment) or death. When these tumors are surgically removed or decompressed, the prognosis for life seems to be quite good. Malignant change in these lesions is very rare.", "contents": "Brain hamartomas and tumors associated with tuberous sclerosis. Ventricular tumors of tuberous sclerosis are almost always hamartomas or benign tumors occurring predominantly in children or young adults. No clear gross or histological distinctions between the ventricular \"candle gutterings\" and \"tumors\" have been identified. The clinical presentation may be acute, with the sudden onset of increased intracranial pressure and possible serious morbidity (visual impairment) or death. When these tumors are surgically removed or decompressed, the prognosis for life seems to be quite good. Malignant change in these lesions is very rare."}
{"id": "PMID:460573", "title": "Afferent fibers in the ventral root.", "content": "The dorsal rhizotomy was one of the earliest operations used for the treatment of chronic, intractable, segmental pain. The theoretical basis underlying the use of this procedure was the law of separation of function of the spinal roots that implies, among other things, that sensory fibers are located in the dorsal root and motor fibers are located in the ventral roots. On this basis, it should be possible by cutting the appropriate dorsal roots to relieve pain for any particular part of the body at the cost of making that part of the body anesthetic. Unfortunately, however, the dorsal rhizotomy seems to have a relatively low success rate in that the pain is often not relieved or is relieved for a variable period of time and then returns. Many different reasons may account for these failures, but one reason that is not often considered is that the law of separation of function of the spinal roots may not be accurate. This is pertinent at the present time, because large numbers of ventral root afferent fibers have recently been discovered. This review summarizes the work, both old and new, on ventral root afferent with particular emphasis on possible clinical applications. In particular, it is pointed out that the ventral root afferent fibers are probably present in humans and, if so, they may be involved in explanations for the failure of dorsal rhizotomy to relieve chronic pain. If these fibers are important, then they can be removed by a dorsal root ganglionectomy, which would remove both the dorsal and ventral root afferent fibers, as opposed to a dorsal rhizotomy, which would remove only the dorsal root afferent fibers.", "contents": "Afferent fibers in the ventral root. The dorsal rhizotomy was one of the earliest operations used for the treatment of chronic, intractable, segmental pain. The theoretical basis underlying the use of this procedure was the law of separation of function of the spinal roots that implies, among other things, that sensory fibers are located in the dorsal root and motor fibers are located in the ventral roots. On this basis, it should be possible by cutting the appropriate dorsal roots to relieve pain for any particular part of the body at the cost of making that part of the body anesthetic. Unfortunately, however, the dorsal rhizotomy seems to have a relatively low success rate in that the pain is often not relieved or is relieved for a variable period of time and then returns. Many different reasons may account for these failures, but one reason that is not often considered is that the law of separation of function of the spinal roots may not be accurate. This is pertinent at the present time, because large numbers of ventral root afferent fibers have recently been discovered. This review summarizes the work, both old and new, on ventral root afferent with particular emphasis on possible clinical applications. In particular, it is pointed out that the ventral root afferent fibers are probably present in humans and, if so, they may be involved in explanations for the failure of dorsal rhizotomy to relieve chronic pain. If these fibers are important, then they can be removed by a dorsal root ganglionectomy, which would remove both the dorsal and ventral root afferent fibers, as opposed to a dorsal rhizotomy, which would remove only the dorsal root afferent fibers."}
{"id": "PMID:460576", "title": "HLA antigens in patients with schizophrenic syndromes.", "content": "HLA antigens were studied in 50 patients, 40 males, 10 females and two brothers of patients, all suffering from schizophrenic syndromes. The average duration of illness for the whole group was 18 +/- 1.5  years. The frequency of A10 antigen was increased in the schizophrenic patients as compared with 449 healthy individuals. None of the findings concerning HLA and schizophrenia reported in the literature could be verified in the present study. Two ill brothers comprised in the study proved to have the same HLA phenotype as their respective ill sibling. So far there is no conclusive evidence for association between any HLA antigen and 'schizophrenia'. Further investigations should be concerned with families and not with single patients.", "contents": "HLA antigens in patients with schizophrenic syndromes. HLA antigens were studied in 50 patients, 40 males, 10 females and two brothers of patients, all suffering from schizophrenic syndromes. The average duration of illness for the whole group was 18 +/- 1.5  years. The frequency of A10 antigen was increased in the schizophrenic patients as compared with 449 healthy individuals. None of the findings concerning HLA and schizophrenia reported in the literature could be verified in the present study. Two ill brothers comprised in the study proved to have the same HLA phenotype as their respective ill sibling. So far there is no conclusive evidence for association between any HLA antigen and 'schizophrenia'. Further investigations should be concerned with families and not with single patients."}
{"id": "PMID:460595", "title": "[Thoracic outlet syndromes. Arteriographic aspects].", "content": "Chest outlet syndromes are characterized by pain symptomatology caused by (congenital or acquired reasons) compression of the vasculo-nervous bundle. After a brief mention of the anatomy of the question, the indications and angiographic technique are reported, special reference being made to certain positions and dynamic tests which increase symptomatology and highlight arteriographic findings. These are clearly identifiable in the pictures and offer documentary evidence of the cervical rib syndrome, scalenus syndrome, small pectoral syndrome, the hyperabduction syndrome and the costoclavicular syndrome. Differential diagnosis should consider a number of painful syndromes of the upper extremity of purely neurological interest (involvement of the brachial plexus). Vascular diseases such as arteriosclerosis, Takayasu's disease, etc., osteo-myo-articular and ORL sector diseases should also be taken into account. Arteriography is able to identify with precision the causes of the clinical syndrome and to guide surgical exploration of the supraclavicular fossa with excellent chances of success.", "contents": "[Thoracic outlet syndromes. Arteriographic aspects]. Chest outlet syndromes are characterized by pain symptomatology caused by (congenital or acquired reasons) compression of the vasculo-nervous bundle. After a brief mention of the anatomy of the question, the indications and angiographic technique are reported, special reference being made to certain positions and dynamic tests which increase symptomatology and highlight arteriographic findings. These are clearly identifiable in the pictures and offer documentary evidence of the cervical rib syndrome, scalenus syndrome, small pectoral syndrome, the hyperabduction syndrome and the costoclavicular syndrome. Differential diagnosis should consider a number of painful syndromes of the upper extremity of purely neurological interest (involvement of the brachial plexus). Vascular diseases such as arteriosclerosis, Takayasu's disease, etc., osteo-myo-articular and ORL sector diseases should also be taken into account. Arteriography is able to identify with precision the causes of the clinical syndrome and to guide surgical exploration of the supraclavicular fossa with excellent chances of success."}
{"id": "PMID:460596", "title": "[Extension of total thyroidectomy to the cellulo-lympho-thymic tissue of the anterior superior mediastinum].", "content": "Stress is laid on the importance of the cellulo-lympho-thymic tissue of the anterior-superior mediastinum in demolition surgery for thyroid cancer. For this reason, a straightforward, reliable technique for removing the thyrothymic lamina by the cervical route has been prepared. Experimented on cadavers, this method has been used for superior mediastinic lymphnode exeresis in all cases where it was indicated.", "contents": "[Extension of total thyroidectomy to the cellulo-lympho-thymic tissue of the anterior superior mediastinum]. Stress is laid on the importance of the cellulo-lympho-thymic tissue of the anterior-superior mediastinum in demolition surgery for thyroid cancer. For this reason, a straightforward, reliable technique for removing the thyrothymic lamina by the cervical route has been prepared. Experimented on cadavers, this method has been used for superior mediastinic lymphnode exeresis in all cases where it was indicated."}
{"id": "PMID:460599", "title": "[Intestinal invagination caused by lipoma of the small intestine].", "content": "A case of ileo-colic invagination caused by lipoma of the last loop of the small intestine is reported. The anatomo-clinical features of this neoformation are illustrated and stress is laid on certain special aspects: the rarity (about 300 cases have been described in the literature), the lack of symptomatology and its insidious development without pathognomonic sign up to the occurrence of occlusive episodes. The surgery carried out and the result obtained are reported.", "contents": "[Intestinal invagination caused by lipoma of the small intestine]. A case of ileo-colic invagination caused by lipoma of the last loop of the small intestine is reported. The anatomo-clinical features of this neoformation are illustrated and stress is laid on certain special aspects: the rarity (about 300 cases have been described in the literature), the lack of symptomatology and its insidious development without pathognomonic sign up to the occurrence of occlusive episodes. The surgery carried out and the result obtained are reported."}
{"id": "PMID:460602", "title": "[Acute respiratory insufficiency caused by posttraumatic pulmonary metabolic disorder].", "content": "Six cases of acute respiratory insufficiency are presented. Particular reference is made to the pathogenetic mechanisms of the syndrome and the term ARDS is examined. The definition \"acute respiratory insufficiency due to post-aggressive pulmonary dysmetabolism\" is proposed. An account is also given of satisfactory results obtained by interdepartmental along-to-date lines. It is hoped that knowledge of this often fatal syndrome will not be confined to resuscitation services, but extended to other hospital departments.", "contents": "[Acute respiratory insufficiency caused by posttraumatic pulmonary metabolic disorder]. Six cases of acute respiratory insufficiency are presented. Particular reference is made to the pathogenetic mechanisms of the syndrome and the term ARDS is examined. The definition \"acute respiratory insufficiency due to post-aggressive pulmonary dysmetabolism\" is proposed. An account is also given of satisfactory results obtained by interdepartmental along-to-date lines. It is hoped that knowledge of this often fatal syndrome will not be confined to resuscitation services, but extended to other hospital departments."}
{"id": "PMID:460603", "title": "[Metabolic preparation for surgical operations and the problem of chronic shock].", "content": "Surgical patients with a chronic energy deficit display a particulare metabolic situation known as chronic shock, marked by deficient utilisation of energy. The picture is related to functional disorders of the cell enzymes, following incomplete resynthesis due to the lack of substrates. The main features are: a) lactic metabolic acidosis due to anaerobic oxidation, usually with insufficiency of the Krebs cycle; b) exaggerated protein catabolism, due to utilisation of amino acids as the energy substrate; c) incomplete utilisation of the (mostly endogenous) substrates available, with lactacidaemia, aminoacidaemia, and increased lipidaemia. A rational form of treatment is proposed after careful analysis of these features and their corresponding clinical phenomena.", "contents": "[Metabolic preparation for surgical operations and the problem of chronic shock]. Surgical patients with a chronic energy deficit display a particulare metabolic situation known as chronic shock, marked by deficient utilisation of energy. The picture is related to functional disorders of the cell enzymes, following incomplete resynthesis due to the lack of substrates. The main features are: a) lactic metabolic acidosis due to anaerobic oxidation, usually with insufficiency of the Krebs cycle; b) exaggerated protein catabolism, due to utilisation of amino acids as the energy substrate; c) incomplete utilisation of the (mostly endogenous) substrates available, with lactacidaemia, aminoacidaemia, and increased lipidaemia. A rational form of treatment is proposed after careful analysis of these features and their corresponding clinical phenomena."}
{"id": "PMID:460604", "title": "[Internal arteriovenous fistula in the antecubital region].", "content": "An assessment was made of the anterior cubital region as a vascular approach for primary and alternative dialysis. Reference is made to 36 fistulae in this area within 44 months' survival in the drawing of various conclusions with regard to surgical technique. End anastomosis of the median, cephalic or basilic vein laterally on the brachial or radial artery is recommended as a means of preventing or cutting down the more common complications associated with internal arteriovenous fitulae. The anastomosis should not exceed 6 mm in diameter. Complications led to loss of fistula function. In many cases, however, they did not prevent employment of the region for alternative approaches, such as superficialisation of the basilic vein or prosthesis.", "contents": "[Internal arteriovenous fistula in the antecubital region]. An assessment was made of the anterior cubital region as a vascular approach for primary and alternative dialysis. Reference is made to 36 fistulae in this area within 44 months' survival in the drawing of various conclusions with regard to surgical technique. End anastomosis of the median, cephalic or basilic vein laterally on the brachial or radial artery is recommended as a means of preventing or cutting down the more common complications associated with internal arteriovenous fitulae. The anastomosis should not exceed 6 mm in diameter. Complications led to loss of fistula function. In many cases, however, they did not prevent employment of the region for alternative approaches, such as superficialisation of the basilic vein or prosthesis."}
{"id": "PMID:460606", "title": "[Carotid body tumors. Observation of an unusual case associated with thyroid disease].", "content": "A case of tumour of the carotid glomus associated with diffuse cystic colloid struma of the thyroid is examined. Classification, symptomatological, diagnostic and therapeutic problems are reassessed on the basis of the case in question and the literature. Stress is laid on the importance of carotidography as a decisive examination for preoperative diagnosis of the nature of the case. Some basic problems of surgical treatment are examined and the operating techniques that have proved most valid are proposed.", "contents": "[Carotid body tumors. Observation of an unusual case associated with thyroid disease]. A case of tumour of the carotid glomus associated with diffuse cystic colloid struma of the thyroid is examined. Classification, symptomatological, diagnostic and therapeutic problems are reassessed on the basis of the case in question and the literature. Stress is laid on the importance of carotidography as a decisive examination for preoperative diagnosis of the nature of the case. Some basic problems of surgical treatment are examined and the operating techniques that have proved most valid are proposed."}
{"id": "PMID:460608", "title": "[Perendoscopic retrograde cholangiopancreatography and transhepatic percutaneous cholangiography in the diagnosis of hepato-biliary diseases].", "content": "Reference is made to the world literature and personal experience acquired at the Digestive Endoscopy Centre, 3rd Surgical Clinic, in an assessment of the value of PRC and TPC in the diagnosis of hepatobiliary disease. PRC is ideally employed when conventional examinations have been inconclusive, and especially in icterus, when technical reasons have prevented their execution. TPC may be required in jaundice when PRC has proved impracticable, or if the data it offers concerning the state of the bile ducts upstream from an obstruction are insufficient for the planning of suitable surgical management.", "contents": "[Perendoscopic retrograde cholangiopancreatography and transhepatic percutaneous cholangiography in the diagnosis of hepato-biliary diseases]. Reference is made to the world literature and personal experience acquired at the Digestive Endoscopy Centre, 3rd Surgical Clinic, in an assessment of the value of PRC and TPC in the diagnosis of hepatobiliary disease. PRC is ideally employed when conventional examinations have been inconclusive, and especially in icterus, when technical reasons have prevented their execution. TPC may be required in jaundice when PRC has proved impracticable, or if the data it offers concerning the state of the bile ducts upstream from an obstruction are insufficient for the planning of suitable surgical management."}
{"id": "PMID:460609", "title": "[Esophagogastroplasty in upper esophageal lesions. Apropos of 4 cases].", "content": "Oesophagogastroplasty has been performed in 4 patients in the Turin Department of Emergency Surgery. Three of them presented high neoplastic lesion of the oesophagus, while the fourth was suffering from mediastinic compression due to sclerosing mediastinitis. The technique and results are reported in detail. Indications for surgery for both malignant and benign lesions are presented. The real advantages of the operation with respect to stomach to chest transposition and oesophagocolonplasty which have in the personal series created considerable inconvenience, are discussed. The reasons making it necessary to carry out the operation in two stages, with different modalities, are also discussed. Complications are indicated and postoperative treatment discussed. This plays a notable part in the success of the operation, as does correct technique.", "contents": "[Esophagogastroplasty in upper esophageal lesions. Apropos of 4 cases]. Oesophagogastroplasty has been performed in 4 patients in the Turin Department of Emergency Surgery. Three of them presented high neoplastic lesion of the oesophagus, while the fourth was suffering from mediastinic compression due to sclerosing mediastinitis. The technique and results are reported in detail. Indications for surgery for both malignant and benign lesions are presented. The real advantages of the operation with respect to stomach to chest transposition and oesophagocolonplasty which have in the personal series created considerable inconvenience, are discussed. The reasons making it necessary to carry out the operation in two stages, with different modalities, are also discussed. Complications are indicated and postoperative treatment discussed. This plays a notable part in the success of the operation, as does correct technique."}
{"id": "PMID:460610", "title": "[Surgical treatment of massive pulmonary embolism. Personal cases].", "content": "5 cases of pulmonary embolism are reported. 3 of these were subjected to pulmonary embolectomy with complete success; repeated controls bear witness to the good long-term cardiorespiratory condition. Two patients died, one following angiography, the other at anaesthetic induction; this fact shows the desirability of submitting patients to partial support extracorporeal circulation prior to angiography and, in any cases, before submitting them to anaesthesia. This surgical success obtained in treatment of the three cases shows that embolectomy continues to play a decisive role.", "contents": "[Surgical treatment of massive pulmonary embolism. Personal cases]. 5 cases of pulmonary embolism are reported. 3 of these were subjected to pulmonary embolectomy with complete success; repeated controls bear witness to the good long-term cardiorespiratory condition. Two patients died, one following angiography, the other at anaesthetic induction; this fact shows the desirability of submitting patients to partial support extracorporeal circulation prior to angiography and, in any cases, before submitting them to anaesthesia. This surgical success obtained in treatment of the three cases shows that embolectomy continues to play a decisive role."}
{"id": "PMID:460613", "title": "[Treatment of cancer of the breast with Patey's modified radical mastectomy].", "content": "Patey's operation was used to treat breast cancer in 658 cases between 1956 and 1976 at the Bologna University Surgical Clinic. Follow-up in 562 cases revealed 5-yr and 10-yr survival rates substantially similar to those obtained by Halsted and others. It is felt that the type of operation used is of relative importance for survival, since breast cancer is dependent on other factors that require careful appraisal in each case.", "contents": "[Treatment of cancer of the breast with Patey's modified radical mastectomy]. Patey's operation was used to treat breast cancer in 658 cases between 1956 and 1976 at the Bologna University Surgical Clinic. Follow-up in 562 cases revealed 5-yr and 10-yr survival rates substantially similar to those obtained by Halsted and others. It is felt that the type of operation used is of relative importance for survival, since breast cancer is dependent on other factors that require careful appraisal in each case."}
{"id": "PMID:460614", "title": "[Study of 357 cases of phlebitis of the lower extremities in 2 inter-regional samplings. I. Methods, epidemiological data and analysis of etiopathogenetic elements].", "content": "Using a specially designed record card, 357 cases  of superficial and deep thrombophlebitis and phlebothrombosis of the lower extremities have been investigated. Of these 261 were observed in Piedmont and 96 in the Trentino region. A comparative study of aetiopathogenetic data in the two regions was made possible by means of punched cards and subsequent computer processing. The study was completed by statistical analysis. Apart from the region of birth and residence, sex (prevalently female) and age which in both samples mainly affects subjects aged between 30 and 45, the type of working activity was considered in relation to sex and age, frequency of absenteeism (higher in Piedmont), family history of venous disease, incidence of orthostatism, the use of contraceptive pills, the period in which the first clinical signs occurred with considerable diversity between the two regions, traumas (more frequent in Piedmont) and the incidence of the different causes which was vastly different in the two samples, were assessed. On the basis of the comparative data obtained, an interpretation of the real significance of the results is attempted, reference also being made to the type of population under examination, the Piedmontese being considered of mixed strain and the Trentino pure.", "contents": "[Study of 357 cases of phlebitis of the lower extremities in 2 inter-regional samplings. I. Methods, epidemiological data and analysis of etiopathogenetic elements]. Using a specially designed record card, 357 cases  of superficial and deep thrombophlebitis and phlebothrombosis of the lower extremities have been investigated. Of these 261 were observed in Piedmont and 96 in the Trentino region. A comparative study of aetiopathogenetic data in the two regions was made possible by means of punched cards and subsequent computer processing. The study was completed by statistical analysis. Apart from the region of birth and residence, sex (prevalently female) and age which in both samples mainly affects subjects aged between 30 and 45, the type of working activity was considered in relation to sex and age, frequency of absenteeism (higher in Piedmont), family history of venous disease, incidence of orthostatism, the use of contraceptive pills, the period in which the first clinical signs occurred with considerable diversity between the two regions, traumas (more frequent in Piedmont) and the incidence of the different causes which was vastly different in the two samples, were assessed. On the basis of the comparative data obtained, an interpretation of the real significance of the results is attempted, reference also being made to the type of population under examination, the Piedmontese being considered of mixed strain and the Trentino pure."}
{"id": "PMID:460615", "title": "[Study of 357 cases of phlebitis of the lower extremities in 2 inter-regional samplings. II. Clinical and diagnostic data and therapeutic conclusions].", "content": "In Piedmont, the incidence of superficial and deep forms is the same percentage-wise. In the Trento area, however, deep forms are more common, though no significant differences can be discerned with respect to the side affected, the site (other than that related to the type of phlebitis), or the symptoms. At least one complication was noted in 241-261 and 27-96 cases in Piedmont and Trento respectively. The treatment initially employed proved inadequate. The risks involved are known solely with respect to patients with complications. The resort to surgical management was much more frequent in the Trento region. An attempt is made to evaluate the risk factors associated with thrombophlebitis of the lower limbs, and the measure of agreement between the aetiopathogenetic, clinical and therapeutic data obtained for the two regions.", "contents": "[Study of 357 cases of phlebitis of the lower extremities in 2 inter-regional samplings. II. Clinical and diagnostic data and therapeutic conclusions]. In Piedmont, the incidence of superficial and deep forms is the same percentage-wise. In the Trento area, however, deep forms are more common, though no significant differences can be discerned with respect to the side affected, the site (other than that related to the type of phlebitis), or the symptoms. At least one complication was noted in 241-261 and 27-96 cases in Piedmont and Trento respectively. The treatment initially employed proved inadequate. The risks involved are known solely with respect to patients with complications. The resort to surgical management was much more frequent in the Trento region. An attempt is made to evaluate the risk factors associated with thrombophlebitis of the lower limbs, and the measure of agreement between the aetiopathogenetic, clinical and therapeutic data obtained for the two regions."}
{"id": "PMID:460617", "title": "[Hepatic echinococcosis. Surgical treatment].", "content": "After some general observations on liver echinococcosis (epidemiology, liver topographic anatomy, diagnostics), various surgical techniques proposed for the treatment of this disease are described. Case series observed from 1967 to 1978 and the technique of choice, cystotomy with partial cysto-resection, are presented. The favourable results obtained are underlined.", "contents": "[Hepatic echinococcosis. Surgical treatment]. After some general observations on liver echinococcosis (epidemiology, liver topographic anatomy, diagnostics), various surgical techniques proposed for the treatment of this disease are described. Case series observed from 1967 to 1978 and the technique of choice, cystotomy with partial cysto-resection, are presented. The favourable results obtained are underlined."}
{"id": "PMID:460618", "title": "[Clinical study of a group of vagotomized patients].", "content": "The physiopathological premisses underlying vagotomy and its use in the treatment of gastroduodenal ulcer are examined. It is submitted that the inadequacies and risks of the original technique are reflected in the modifications subsequently introduced into truncular vagotomy. A long-term assessment is made of a series of patients operated for duodenal ulcer by different surgeons using different forms of vagotomy. It is felt that superselective vagotomy is most consonant with the therapeutic objectives and associated with the least risk. In addition vagotomy is indicated in subjects with POPU after gastric section whose local and general condition contraindicate more complicated and serious measures, such as degastroenterostomy with further resection.", "contents": "[Clinical study of a group of vagotomized patients]. The physiopathological premisses underlying vagotomy and its use in the treatment of gastroduodenal ulcer are examined. It is submitted that the inadequacies and risks of the original technique are reflected in the modifications subsequently introduced into truncular vagotomy. A long-term assessment is made of a series of patients operated for duodenal ulcer by different surgeons using different forms of vagotomy. It is felt that superselective vagotomy is most consonant with the therapeutic objectives and associated with the least risk. In addition vagotomy is indicated in subjects with POPU after gastric section whose local and general condition contraindicate more complicated and serious measures, such as degastroenterostomy with further resection."}
{"id": "PMID:460619", "title": "[Diagnosis and treatment of traumatic lesions of the pancreas].", "content": "A survey of the main series in the world literature and personal data concerning injuries to the pancreas is followed by the suggestion that the risk of pseudocyst or pancreatitis means that in all cases where injury to the pancreas is suspected, and resolution is slow, laparotomy must be performed, coupled with careful exploration of the lesser omentum and precise evaluation of possible lesions, prior to suitable surgical management. Medical therapy is also of prime importance. Its main feature is the administration of a protease inhibitor to prevent pre- and post-operative enzymatic toxaemia.", "contents": "[Diagnosis and treatment of traumatic lesions of the pancreas]. A survey of the main series in the world literature and personal data concerning injuries to the pancreas is followed by the suggestion that the risk of pseudocyst or pancreatitis means that in all cases where injury to the pancreas is suspected, and resolution is slow, laparotomy must be performed, coupled with careful exploration of the lesser omentum and precise evaluation of possible lesions, prior to suitable surgical management. Medical therapy is also of prime importance. Its main feature is the administration of a protease inhibitor to prevent pre- and post-operative enzymatic toxaemia."}
{"id": "PMID:460623", "title": "[Cystoadenocarcinoma of the pancreas].", "content": "A case of papilliferous cystadenocarcinoma of the body and tail of the pancreas marked by unusually slow progresion is presented. It is pointed out that total resection, of the tumour is always necessary. This is also true of cystadenoma, which has a tendency to become malignant.", "contents": "[Cystoadenocarcinoma of the pancreas]. A case of papilliferous cystadenocarcinoma of the body and tail of the pancreas marked by unusually slow progresion is presented. It is pointed out that total resection, of the tumour is always necessary. This is also true of cystadenoma, which has a tendency to become malignant."}
{"id": "PMID:460627", "title": "[Vascularization of the jejunal loop].", "content": "Vascularization of the first 60 cm of the jejunum is considered on the basis of a necroptic study of 100 cases. Attention is called to the possibility that surgical failure in ansa transposition techniques may be due to anatomical variations in the first jejunal branches of the superior mesenteric.", "contents": "[Vascularization of the jejunal loop]. Vascularization of the first 60 cm of the jejunum is considered on the basis of a necroptic study of 100 cases. Attention is called to the possibility that surgical failure in ansa transposition techniques may be due to anatomical variations in the first jejunal branches of the superior mesenteric."}
{"id": "PMID:460629", "title": "[Apropos of 2 unusual variations of origin and course of the deep femoral artery].", "content": "Two cases have been observed in which the deep femoral artery originated at the medial surface of the femoral and surrounded medially the femoral vein before entering its distribution area. The literature is examined and stress laid on the rarity of the abnormality and its importance as a possible factor in phlebothrombosis.", "contents": "[Apropos of 2 unusual variations of origin and course of the deep femoral artery]. Two cases have been observed in which the deep femoral artery originated at the medial surface of the femoral and surrounded medially the femoral vein before entering its distribution area. The literature is examined and stress laid on the rarity of the abnormality and its importance as a possible factor in phlebothrombosis."}
{"id": "PMID:460630", "title": "[Posterior gastro-esophago-phreno-plasty (PGEPP) in the treatment of hiatal hernia and of esophagogastric reflux. Experience in over 500 cases. Anterior gastro-esophago-phreno-plasty (AGEPP) in the prevention of sliding hiatal hernia and of esophagogastric reflux. Experience in over 300 cases].", "content": "On the basis of the anatomophysiological assumption that the abdominal oesophagus is kept in its seat by the meso-oesophagus and that the complex functional role of the gastro-oesophageal junction is conditioned essentially by the inferior oesophageal sphincter under the influence of intra-abdominal and endogastric pressure variables, posterior gastro-oesophagophreno-plasty (p.g.p.p.) is proposed to offset the destruction or severe insufficiency of the meso-oesophagus and hence for the treatment of hiatal hernia and of regurgitation. This retro-oesophageal tuberous valve involves simultaneous fixation of the stomach and oesophagus to the pillars of the diaphragm, first on the right and then on the left, and the fixation of the fundus to the left diaphragmatic dome. Anterior gastro-oesophago-phreno-plasty is proposed on the basis of the finding that damage of any kind to the meso-oesophagus can cause the oesophagus to rise in the chest and thus disturb inferior sphincter function and possibly lead to the onset of regurgitation and hernia. The pre-oesophageal tuberous valve employed involves simultaneous fixation of stomach and oesophagus to the pillars of the diaphragm, first left and then right, and the fixation of the fundus to the left diaphragmatic dome. Anterior plasty is almost always confined to patients who have undergone vagotomy of the trunk without complex isolation of the oesophagus.", "contents": "[Posterior gastro-esophago-phreno-plasty (PGEPP) in the treatment of hiatal hernia and of esophagogastric reflux. Experience in over 500 cases. Anterior gastro-esophago-phreno-plasty (AGEPP) in the prevention of sliding hiatal hernia and of esophagogastric reflux. Experience in over 300 cases]. On the basis of the anatomophysiological assumption that the abdominal oesophagus is kept in its seat by the meso-oesophagus and that the complex functional role of the gastro-oesophageal junction is conditioned essentially by the inferior oesophageal sphincter under the influence of intra-abdominal and endogastric pressure variables, posterior gastro-oesophagophreno-plasty (p.g.p.p.) is proposed to offset the destruction or severe insufficiency of the meso-oesophagus and hence for the treatment of hiatal hernia and of regurgitation. This retro-oesophageal tuberous valve involves simultaneous fixation of the stomach and oesophagus to the pillars of the diaphragm, first on the right and then on the left, and the fixation of the fundus to the left diaphragmatic dome. Anterior gastro-oesophago-phreno-plasty is proposed on the basis of the finding that damage of any kind to the meso-oesophagus can cause the oesophagus to rise in the chest and thus disturb inferior sphincter function and possibly lead to the onset of regurgitation and hernia. The pre-oesophageal tuberous valve employed involves simultaneous fixation of stomach and oesophagus to the pillars of the diaphragm, first left and then right, and the fixation of the fundus to the left diaphragmatic dome. Anterior plasty is almost always confined to patients who have undergone vagotomy of the trunk without complex isolation of the oesophagus."}
{"id": "PMID:460631", "title": "[Tuberculosis of the breast. Considerations on a case].", "content": "A recently observed case of T.B. of the breast is presented and the relevant literature is examined. View are expressed concerning the aetiopathogenesis, diagnosis and treatment of this unusual form, particular attention being given to the difficulty of distinguishing it from carcinoma. Stress is laid on the importance of surgical management, this being indispensable for both correct diagnosis and for treatment.", "contents": "[Tuberculosis of the breast. Considerations on a case]. A recently observed case of T.B. of the breast is presented and the relevant literature is examined. View are expressed concerning the aetiopathogenesis, diagnosis and treatment of this unusual form, particular attention being given to the difficulty of distinguishing it from carcinoma. Stress is laid on the importance of surgical management, this being indispensable for both correct diagnosis and for treatment."}
{"id": "PMID:460633", "title": "[Pharmacokinetics of a new aminoglycoside: kanendomycin].", "content": "The pharmacokinetics of kanendomycin, a new aminoglycoside derived from kanamycin, has been assessed in 15 volunteers after i.m. administration of 100 and 300 mg and i.v. administration of 100 mg. Analysis of the tricompartmental model adopted for the pharmacokinetic study showed that kanendomycin half-life is similar and perhaps superior to that of gentamycin. The antibiotic's bioavailability is of the order of 70-80% of the dose used.", "contents": "[Pharmacokinetics of a new aminoglycoside: kanendomycin]. The pharmacokinetics of kanendomycin, a new aminoglycoside derived from kanamycin, has been assessed in 15 volunteers after i.m. administration of 100 and 300 mg and i.v. administration of 100 mg. Analysis of the tricompartmental model adopted for the pharmacokinetic study showed that kanendomycin half-life is similar and perhaps superior to that of gentamycin. The antibiotic's bioavailability is of the order of 70-80% of the dose used."}
{"id": "PMID:460649", "title": "[Cryoglobulinemia syndrome and liver diseases. Case reports].", "content": "Six cases of an association of liver disease with a cryoglobulinaemia syndrome are described. An account of their clinical, body fluid and anatomopathological pictures is followed by a discussion of their possible causes in the light of the relevant literature. It is felt that no assessment can be made of the primary nature of either disease in associations of this kind. In addition to cases in which prior liver disease or exposure to hepatitis virus can be shown, in fact, there are other situations in which liver disease was present before the manifestation of cryoglobulinaemia. Repeated antigen stimulus triggers the antibody response leading to the formation of cryoprecipitating complexes. In most cases, the nature of such antigen is not known, since HB virus or any other virus, bacterial product, or cell catabolism product either eliminated in insufficient quantities, or produced in abnormal quantities, may be responsible.", "contents": "[Cryoglobulinemia syndrome and liver diseases. Case reports]. Six cases of an association of liver disease with a cryoglobulinaemia syndrome are described. An account of their clinical, body fluid and anatomopathological pictures is followed by a discussion of their possible causes in the light of the relevant literature. It is felt that no assessment can be made of the primary nature of either disease in associations of this kind. In addition to cases in which prior liver disease or exposure to hepatitis virus can be shown, in fact, there are other situations in which liver disease was present before the manifestation of cryoglobulinaemia. Repeated antigen stimulus triggers the antibody response leading to the formation of cryoprecipitating complexes. In most cases, the nature of such antigen is not known, since HB virus or any other virus, bacterial product, or cell catabolism product either eliminated in insufficient quantities, or produced in abnormal quantities, may be responsible."}
{"id": "PMID:460650", "title": "[Persistent chronic hepatitis. Anatomical and functional study].", "content": "A functional study was made of 40 patients with bioptically diagnosed CPH. Fractional BSF clearance was the most frequently altered test and its correlation with the anatomical damage was significant. In particular, the fractional uptake clearance (K1) gave a pointer to both the presence of liver damage and its type. Elimination clearance (K2) may also be useful, especially in the differentiation of histological pictures on the borderline between CAH and CPH. In the evaluation of CPH, therefore, BSF clearance is of marked assistance both in rounding out the histological diagnosis, and in cutting down the need for control biopsies.", "contents": "[Persistent chronic hepatitis. Anatomical and functional study]. A functional study was made of 40 patients with bioptically diagnosed CPH. Fractional BSF clearance was the most frequently altered test and its correlation with the anatomical damage was significant. In particular, the fractional uptake clearance (K1) gave a pointer to both the presence of liver damage and its type. Elimination clearance (K2) may also be useful, especially in the differentiation of histological pictures on the borderline between CAH and CPH. In the evaluation of CPH, therefore, BSF clearance is of marked assistance both in rounding out the histological diagnosis, and in cutting down the need for control biopsies."}
{"id": "PMID:460652", "title": "[Malignant histiocytosis. Histiocytic medullary reticulosis].", "content": "The Authors report a case of malignant histiocytosis apparently localized only in the spleen. The diagnosis of the disease, characterized at the onset by moderate leukopenia and thrombocytopenia, was made possible by the histopathologic examination of the spleen following splenectomy. The disease shows a chronic course. The Authors discuss the clinical and histological features of the case.", "contents": "[Malignant histiocytosis. Histiocytic medullary reticulosis]. The Authors report a case of malignant histiocytosis apparently localized only in the spleen. The diagnosis of the disease, characterized at the onset by moderate leukopenia and thrombocytopenia, was made possible by the histopathologic examination of the spleen following splenectomy. The disease shows a chronic course. The Authors discuss the clinical and histological features of the case."}
{"id": "PMID:460653", "title": "[Anastomotic recurrence of colo-rectal cancer. Anatomo-surgical study].", "content": "A study was made of 20 patients operated for colorectal cancer with the aid of the University of Chieti Department of Pathological Anatomy. The histological preparations were carefully assessed for intramural diffusion of the tumour, and for the presence of hyperplastic and dysplastic processes in apparently healthy mucosa. The results were in full agreement with those reported in the literature.", "contents": "[Anastomotic recurrence of colo-rectal cancer. Anatomo-surgical study]. A study was made of 20 patients operated for colorectal cancer with the aid of the University of Chieti Department of Pathological Anatomy. The histological preparations were carefully assessed for intramural diffusion of the tumour, and for the presence of hyperplastic and dysplastic processes in apparently healthy mucosa. The results were in full agreement with those reported in the literature."}
{"id": "PMID:460654", "title": "[Case of secondary syphilis with bone localization].", "content": "On the basis of a case of secondary syphilis with bone involvement, this rare manifestation of the secondary period is discussed and reported data reviewed.", "contents": "[Case of secondary syphilis with bone localization]. On the basis of a case of secondary syphilis with bone involvement, this rare manifestation of the secondary period is discussed and reported data reviewed."}
{"id": "PMID:460681", "title": "Cortical afferents to the prefrontal cortex of the cat: a study with the horseradish peroxidase technique.", "content": "Following horseradish peroxidase (HRP) injections into different areas within the prefrontal cortex (PFC) of the cat, labeled neurons were found in the cingulate and insular cortex. These results demonstrate that the cat's prefrontal cortex is reached directly from these cortical regions, and that the observed cortical projections are similar to those detected in the monkey's prefrontal cortex.", "contents": "Cortical afferents to the prefrontal cortex of the cat: a study with the horseradish peroxidase technique. Following horseradish peroxidase (HRP) injections into different areas within the prefrontal cortex (PFC) of the cat, labeled neurons were found in the cingulate and insular cortex. These results demonstrate that the cat's prefrontal cortex is reached directly from these cortical regions, and that the observed cortical projections are similar to those detected in the monkey's prefrontal cortex."}
{"id": "PMID:460682", "title": "The precise origin of the tectospinal pathway in three common laboratory animals: a study using the horseradish peroxidase method.", "content": "The horseradish peroxidase tracing method has been used to study the cells of origin of the tectospinal projections in the opossum, the tree shrew, and the cat. The present data show that only those collicular neurons which occupy the deep (ventral to the stratum opticum) tectal laminae send axons to the cervical spinal cord. In particular, layer IV contains the greatest number of spinal projecting neurons. Our results also reveal that while only the large sized collicular neurons project upon the cervical spinal cord in the opossum and the tree shrew, neurons comprising several different size categories do so in the cat. We thus suggest that several different descending channels exist over which the superior colliculus can influence the neck musculature in the cat.", "contents": "The precise origin of the tectospinal pathway in three common laboratory animals: a study using the horseradish peroxidase method. The horseradish peroxidase tracing method has been used to study the cells of origin of the tectospinal projections in the opossum, the tree shrew, and the cat. The present data show that only those collicular neurons which occupy the deep (ventral to the stratum opticum) tectal laminae send axons to the cervical spinal cord. In particular, layer IV contains the greatest number of spinal projecting neurons. Our results also reveal that while only the large sized collicular neurons project upon the cervical spinal cord in the opossum and the tree shrew, neurons comprising several different size categories do so in the cat. We thus suggest that several different descending channels exist over which the superior colliculus can influence the neck musculature in the cat."}
{"id": "PMID:460683", "title": "Neuronal degeneration induced by stereotaxic injection of beta-bungarotoxin into rat brain.", "content": "The presynaptic protein neurotoxin beta-bungarotoxin (beta-bgt) caused degeneration of neurons, following stereotaxic administration into rat brain. Focal lesions were induced with as little as 0.1 ng (5 fmol) of beta-bgt; indicating that the toxin is over 10(6) times more potent than kainic acid. More extensive studies in the septo-hippocampal system showed beta-bgt lesions affected both cell bodies and nerve terminals and were not neurotransmitter-specific. The endogenous phospholipase A2 activity of beta-bgt did not simply account for the creation of lesions since an acidic phospholipase A isoenzyme from Vipera russellii was virtually inactive in causing neuronal damage.", "contents": "Neuronal degeneration induced by stereotaxic injection of beta-bungarotoxin into rat brain. The presynaptic protein neurotoxin beta-bungarotoxin (beta-bgt) caused degeneration of neurons, following stereotaxic administration into rat brain. Focal lesions were induced with as little as 0.1 ng (5 fmol) of beta-bgt; indicating that the toxin is over 10(6) times more potent than kainic acid. More extensive studies in the septo-hippocampal system showed beta-bgt lesions affected both cell bodies and nerve terminals and were not neurotransmitter-specific. The endogenous phospholipase A2 activity of beta-bgt did not simply account for the creation of lesions since an acidic phospholipase A isoenzyme from Vipera russellii was virtually inactive in causing neuronal damage."}
{"id": "PMID:460684", "title": "Coordinated rhythmic bursting in respiratory and locomotor muscle nerves in the spinal rabbit.", "content": "In unanaesthetized, curarized spinal rabbits (C2 level) treated with Niamide and DOPA, rhythmic activities were recorded from the phrenic nerves; close coordination was observed between the phrenic bursts and the locomotor bursts which developed in hindlimb muscle nerves. The frequency of phrenic bursts was reduced after a second spinal transection at the Th12 level, while rhythms in the hindlimb remained unchanged. It thus appears that in the spinal preparation and under certain pharmacological conditions, phrenic bursts generated by the cervico-thoracic spinal cord can be driven by the lumbar generators of locomotion; spinal links thus exist between these hindlimb locomotion generators and spinal interneuronal networks involved in phrenic motoneuronal activation, may be via hindlimb forelimb driving.", "contents": "Coordinated rhythmic bursting in respiratory and locomotor muscle nerves in the spinal rabbit. In unanaesthetized, curarized spinal rabbits (C2 level) treated with Niamide and DOPA, rhythmic activities were recorded from the phrenic nerves; close coordination was observed between the phrenic bursts and the locomotor bursts which developed in hindlimb muscle nerves. The frequency of phrenic bursts was reduced after a second spinal transection at the Th12 level, while rhythms in the hindlimb remained unchanged. It thus appears that in the spinal preparation and under certain pharmacological conditions, phrenic bursts generated by the cervico-thoracic spinal cord can be driven by the lumbar generators of locomotion; spinal links thus exist between these hindlimb locomotion generators and spinal interneuronal networks involved in phrenic motoneuronal activation, may be via hindlimb forelimb driving."}
{"id": "PMID:460685", "title": "Presynaptic dendrites and serial synapses in the intermediate and deep layers of the cat superior coliculus.", "content": "Presynaptic dendrites (PSDs) which participate in the serial synapses have frequently been found in the intermediate and deep layers of the cat superior colliculus. The PSDs are presynaptic to small dendritic shafts or spines with symmetrical membrane thikening, and postsynaptic to axon terminals with asymmetrical synaptic contact. Two types of the axon terminals are observed, both of which contain pleomorphic vesicles.", "contents": "Presynaptic dendrites and serial synapses in the intermediate and deep layers of the cat superior coliculus. Presynaptic dendrites (PSDs) which participate in the serial synapses have frequently been found in the intermediate and deep layers of the cat superior colliculus. The PSDs are presynaptic to small dendritic shafts or spines with symmetrical membrane thikening, and postsynaptic to axon terminals with asymmetrical synaptic contact. Two types of the axon terminals are observed, both of which contain pleomorphic vesicles."}
{"id": "PMID:460686", "title": "The nervous system-specific S-100 antigen in cerebrospinal fluid of multiple sclerosis patients.", "content": "The nervous system-specific S-100 antigen has been found in cerebrospinal fluid (CSF) of 13 out of 18 patients with multiple sclerosis (MS), whereas it was undetectable in either of the 11 control patients with minor psychic disturbances or with neurological disorders not usually associated with apparent parenchymal lesion. The levels of the antigen appeared to be higher in CSF of patients in the acute phase of the disease. Though the small number of cases hampers final statements, the S-100 in CSF might serve as a possible index of active cell injury in the central nervous system underlying the pathogenesis of MS.", "contents": "The nervous system-specific S-100 antigen in cerebrospinal fluid of multiple sclerosis patients. The nervous system-specific S-100 antigen has been found in cerebrospinal fluid (CSF) of 13 out of 18 patients with multiple sclerosis (MS), whereas it was undetectable in either of the 11 control patients with minor psychic disturbances or with neurological disorders not usually associated with apparent parenchymal lesion. The levels of the antigen appeared to be higher in CSF of patients in the acute phase of the disease. Though the small number of cases hampers final statements, the S-100 in CSF might serve as a possible index of active cell injury in the central nervous system underlying the pathogenesis of MS."}
{"id": "PMID:460687", "title": "Endogenous levels of tryptophan, serotonin and 5-hydroxyindole acetic acid in the developing brain of the cat.", "content": "Using spectrofluorimetric methods, endogenous levels of tryptophan (TRP), serotonin (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined in various structures of the growing brain of cats. After one month, 5-HT metabolism is comparable to that observed in adult cats. The third week after birth is marked by increasing levels of TRP and 5-HIAA and seems to be a critical period for the maturation of serotoninergic neurons.", "contents": "Endogenous levels of tryptophan, serotonin and 5-hydroxyindole acetic acid in the developing brain of the cat. Using spectrofluorimetric methods, endogenous levels of tryptophan (TRP), serotonin (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined in various structures of the growing brain of cats. After one month, 5-HT metabolism is comparable to that observed in adult cats. The third week after birth is marked by increasing levels of TRP and 5-HIAA and seems to be a critical period for the maturation of serotoninergic neurons."}
{"id": "PMID:460688", "title": "Rapid behavioural and biochemical effects of tetanus toxin microinjected into the substantia nigra: a dual role for GABA?", "content": "Tetanus toxin (200--250 mouse lethal doses) microinjected unilaterally into the substantia nigra produced rapid behavioural and biochemical effects. If the toxin was injected into the rostral part of the substantia nigra (SN), contralateral turning was observed. Raised ipsilateral striatal levels of the dopamine (DA) metabolite, homovanillic acid (HVA), indicated that this turning was due to activation of the nigro-striatal dopamine system. Caudal nigral injections of tetanus toxin produced ipsilateral turning. Biochemical estimations suggest that this behaviour change was mediated by a non-dopamine (non-DA) pathway.", "contents": "Rapid behavioural and biochemical effects of tetanus toxin microinjected into the substantia nigra: a dual role for GABA? Tetanus toxin (200--250 mouse lethal doses) microinjected unilaterally into the substantia nigra produced rapid behavioural and biochemical effects. If the toxin was injected into the rostral part of the substantia nigra (SN), contralateral turning was observed. Raised ipsilateral striatal levels of the dopamine (DA) metabolite, homovanillic acid (HVA), indicated that this turning was due to activation of the nigro-striatal dopamine system. Caudal nigral injections of tetanus toxin produced ipsilateral turning. Biochemical estimations suggest that this behaviour change was mediated by a non-dopamine (non-DA) pathway."}
{"id": "PMID:460689", "title": "Evidence for presynaptic cholinergic receptors on dopaminergic terminals: degeneration studies with 6-hydroxydopamine.", "content": "Intraventricular injections of 6-hydroxydopamine (6-OHDA) caused a significant decrease in the tyrosine hydroxylase of striatal synaptosomes of rats, without influencing GABA levels. Significant decreases in the muscarinic and nicotinic receptors also occurred in this preparation after 3 days. The receptors were measured by specific binding studies with N-methylatropine and alpha-bungarotoxin respectively. These results indicate that muscarinic and nicotinic receptors are located on the dopaminergic nerve terminals of corpus striatum.", "contents": "Evidence for presynaptic cholinergic receptors on dopaminergic terminals: degeneration studies with 6-hydroxydopamine. Intraventricular injections of 6-hydroxydopamine (6-OHDA) caused a significant decrease in the tyrosine hydroxylase of striatal synaptosomes of rats, without influencing GABA levels. Significant decreases in the muscarinic and nicotinic receptors also occurred in this preparation after 3 days. The receptors were measured by specific binding studies with N-methylatropine and alpha-bungarotoxin respectively. These results indicate that muscarinic and nicotinic receptors are located on the dopaminergic nerve terminals of corpus striatum."}
{"id": "PMID:460690", "title": "Ammon's horn commissural responses in young rabbits.", "content": "Different responses evoked by stimulation of contralateral hippocampus at various levels are examined in new-born rabbits up to 1 month of age. Commissural fibres coming from the CA3-CA4 reach the contralateral regio superior (CA1) essentially at the st. oriens levels. CA1 responses elicited by stimulation of the contralateral CA1 in the st. oriens (commissural CA3 fibres) or in the st. radiatum (Schaffer's collaterals) are present at any age. For the regio inferior (CA3-CA4), the distribution of commissural fibres is more diffuse. Before 5--6 days, CA3-CA4 fields do not respond to a contralateral stimulation and do not give contralateral responses, showing that fibres and cells are not yet organized.", "contents": "Ammon's horn commissural responses in young rabbits. Different responses evoked by stimulation of contralateral hippocampus at various levels are examined in new-born rabbits up to 1 month of age. Commissural fibres coming from the CA3-CA4 reach the contralateral regio superior (CA1) essentially at the st. oriens levels. CA1 responses elicited by stimulation of the contralateral CA1 in the st. oriens (commissural CA3 fibres) or in the st. radiatum (Schaffer's collaterals) are present at any age. For the regio inferior (CA3-CA4), the distribution of commissural fibres is more diffuse. Before 5--6 days, CA3-CA4 fields do not respond to a contralateral stimulation and do not give contralateral responses, showing that fibres and cells are not yet organized."}
{"id": "PMID:460691", "title": "Conductance of end-plate channels is voltage dependent.", "content": "The amplitude of miniature end-plate currents (MEPCs) and end-plate currents generated by iontophoresis of acetylcholine (ACh) were recorded in voltage-clamped toad sartorius fibres. Single channel conductance was determined from analysis of current fluctuations. In normal solution, but even more so in solutions containing lithium instead of sodium, both the peak conductance during a miniature end-plate current and the conductance of individual end-plate channels varied with membrane potential, becoming less at more hyperpolarized potentials. Single channel conductance was not influenced by membrane potential in solutions containing potassium or caesium instead of sodium. It was concluded that the nature of the ions passing through an end-plate channel influences its conductance.", "contents": "Conductance of end-plate channels is voltage dependent. The amplitude of miniature end-plate currents (MEPCs) and end-plate currents generated by iontophoresis of acetylcholine (ACh) were recorded in voltage-clamped toad sartorius fibres. Single channel conductance was determined from analysis of current fluctuations. In normal solution, but even more so in solutions containing lithium instead of sodium, both the peak conductance during a miniature end-plate current and the conductance of individual end-plate channels varied with membrane potential, becoming less at more hyperpolarized potentials. Single channel conductance was not influenced by membrane potential in solutions containing potassium or caesium instead of sodium. It was concluded that the nature of the ions passing through an end-plate channel influences its conductance."}
{"id": "PMID:460692", "title": "End-plate channels behave as neutral site channels.", "content": "The voltage sensitivity and concentration dependence of single channel conductance at the motor end-plate in the presence of different alkali cations have been compared with the predictions of different permeation models. The models have been developed, using the diffusional approach, to cater for three cations and anion permeability has been assumed to be insignificant. It was found that a neutral site channel model provided the best fit to the experimental data. The analyses also implied a high field strength sequence for the equilibrium selectively of the sites.", "contents": "End-plate channels behave as neutral site channels. The voltage sensitivity and concentration dependence of single channel conductance at the motor end-plate in the presence of different alkali cations have been compared with the predictions of different permeation models. The models have been developed, using the diffusional approach, to cater for three cations and anion permeability has been assumed to be insignificant. It was found that a neutral site channel model provided the best fit to the experimental data. The analyses also implied a high field strength sequence for the equilibrium selectively of the sites."}
{"id": "PMID:460693", "title": "Absence of the major dense line in myelin of the mutant mouse \"shiverer\".", "content": "The myelin of the central nervous system (CNS) of the mutant mouse Shiverer is characterized by the absence of the major dense line (MDL). The intraperiod line, as seen in conventional electron micrographs and in freeze-fractured replicas, appears normal. Peripheral myelin, as seen in ventral and dorsal roots of spinal cord, is unaffected by the mutation. During the period of active myelination, the cytoplasm of most oligodendrocytes (ODs) is packed with electron-lucent vacuoles in continuity with the Golgi apparatus and with bundles of microtubules. It is concluded that a metabolic pathway possibly involving the Golgi apparatus, and contributing to the formation of the MDL is selectively affected in this mutant.", "contents": "Absence of the major dense line in myelin of the mutant mouse \"shiverer\". The myelin of the central nervous system (CNS) of the mutant mouse Shiverer is characterized by the absence of the major dense line (MDL). The intraperiod line, as seen in conventional electron micrographs and in freeze-fractured replicas, appears normal. Peripheral myelin, as seen in ventral and dorsal roots of spinal cord, is unaffected by the mutation. During the period of active myelination, the cytoplasm of most oligodendrocytes (ODs) is packed with electron-lucent vacuoles in continuity with the Golgi apparatus and with bundles of microtubules. It is concluded that a metabolic pathway possibly involving the Golgi apparatus, and contributing to the formation of the MDL is selectively affected in this mutant."}
{"id": "PMID:460694", "title": "Synaptosomal uptake and release of dopamine in substantia nigra: effects of gamma-aminobutyric acid and substance P.", "content": "Nigral tissue prepared as synaptosomes demonstrates both high and low affinity uptake of [3H]dopamine. Recently accumulated [3H]dopamine is releasable by 35 mN K+. Substance P increases both uptake and release of dopamine by nigral synaptosomes; gamma-aminobutyric acid (GABA) inhibits release with no effect on uptake at concentrations less than 10-(4) M. In the striatum, substance P inhibits both uptake and release of dopamine. The results support the existence of dopamine-containing terminals in substantia nigra tissue. The differences in response to substance P and GABA found between nigra and striatum may reflect structural differences in dopamine-containing processes in these areas, related to their proposed origin as dendtritic (substantia nigra) and axonal (striatal) terminal.", "contents": "Synaptosomal uptake and release of dopamine in substantia nigra: effects of gamma-aminobutyric acid and substance P. Nigral tissue prepared as synaptosomes demonstrates both high and low affinity uptake of [3H]dopamine. Recently accumulated [3H]dopamine is releasable by 35 mN K+. Substance P increases both uptake and release of dopamine by nigral synaptosomes; gamma-aminobutyric acid (GABA) inhibits release with no effect on uptake at concentrations less than 10-(4) M. In the striatum, substance P inhibits both uptake and release of dopamine. The results support the existence of dopamine-containing terminals in substantia nigra tissue. The differences in response to substance P and GABA found between nigra and striatum may reflect structural differences in dopamine-containing processes in these areas, related to their proposed origin as dendtritic (substantia nigra) and axonal (striatal) terminal."}
{"id": "PMID:460695", "title": "Topographical distribution of substance P in the cat substantia nigra.", "content": "The substance P (SP) content in cat substantia nigra (SN) was estimated by radioimmunoassay in microdiscs punched on successive frontal sections. Only slight differences were seen in the regional distribution of SP within the SN. The highest content was found in the pars reticulata (20.7 pmol/mg protein) which was two times higher than in the pars compacta and pars lateralis. Identical results were found in the right and left part of the SN. Out of this area, as pedunculus cerebri and zona incerta, very low concentrations were detected.", "contents": "Topographical distribution of substance P in the cat substantia nigra. The substance P (SP) content in cat substantia nigra (SN) was estimated by radioimmunoassay in microdiscs punched on successive frontal sections. Only slight differences were seen in the regional distribution of SP within the SN. The highest content was found in the pars reticulata (20.7 pmol/mg protein) which was two times higher than in the pars compacta and pars lateralis. Identical results were found in the right and left part of the SN. Out of this area, as pedunculus cerebri and zona incerta, very low concentrations were detected."}
{"id": "PMID:460697", "title": "Noradrenaline and ethanol intake in the rat.", "content": "Intracerebral injection of 4 microgram of the neurotoxin 6-hydroxydopamine (6-OHDA) was used to deplete forebrain noradrenaline (NA) in rats to less than 5% of control values without affecting brain dopamine (DA) and the oral consumption of ethanol examined. Control rats showed a progressive increase in their intake of a 15% ethanol solution and after 15 days were consuming large quantities. This increase did not occur in NA depleted rats, which after 15 days had consumed no more than a few millilitres of the solution in total. The results are discussed in terms of a central noradrenergic basis of ethanol reward.", "contents": "Noradrenaline and ethanol intake in the rat. Intracerebral injection of 4 microgram of the neurotoxin 6-hydroxydopamine (6-OHDA) was used to deplete forebrain noradrenaline (NA) in rats to less than 5% of control values without affecting brain dopamine (DA) and the oral consumption of ethanol examined. Control rats showed a progressive increase in their intake of a 15% ethanol solution and after 15 days were consuming large quantities. This increase did not occur in NA depleted rats, which after 15 days had consumed no more than a few millilitres of the solution in total. The results are discussed in terms of a central noradrenergic basis of ethanol reward."}
{"id": "PMID:460699", "title": "Topographical representation in rabbit cerebellar flocculus for various afferent inputs from the brainstem investigated by means of retrograde axonal transport of horseradish peroxidase.", "content": "A small amount of horseradish peroxidase (HRP) was injected into several small areas of the flocculus and adjacent ventral paraflocculus of albino rabbits. Labeled cells were surveyed through the inferior olive, vestibular nuclear complex, nucleus reticularis tegmenti pontis, pontine nucleus and abducens nucleus. Distribution of labeled neurons in these structures varied depending on the location of injection sites, indicating the existence of a fine topographical organization for afferent inputs in the flocculus and adjacent ventral paraflocculus.", "contents": "Topographical representation in rabbit cerebellar flocculus for various afferent inputs from the brainstem investigated by means of retrograde axonal transport of horseradish peroxidase. A small amount of horseradish peroxidase (HRP) was injected into several small areas of the flocculus and adjacent ventral paraflocculus of albino rabbits. Labeled cells were surveyed through the inferior olive, vestibular nuclear complex, nucleus reticularis tegmenti pontis, pontine nucleus and abducens nucleus. Distribution of labeled neurons in these structures varied depending on the location of injection sites, indicating the existence of a fine topographical organization for afferent inputs in the flocculus and adjacent ventral paraflocculus."}
{"id": "PMID:460700", "title": "Enhancing the anterograde movement of HRP to label sparse neuronal projections.", "content": "By combining techniques designed to increase the amount of horseradish peroxidase (HRP) available for transport and to improve the sensitivity of the reaction for detecting HRP, we have produced an anterograde HRP technique suitable for labeling sparse efferent projections. The efficacy of this light, microscopic technique was demonstrated by lebeling the hippocampal commissural and crossed temporo-ammonic projections to the dentate gyrus of the rat.", "contents": "Enhancing the anterograde movement of HRP to label sparse neuronal projections. By combining techniques designed to increase the amount of horseradish peroxidase (HRP) available for transport and to improve the sensitivity of the reaction for detecting HRP, we have produced an anterograde HRP technique suitable for labeling sparse efferent projections. The efficacy of this light, microscopic technique was demonstrated by lebeling the hippocampal commissural and crossed temporo-ammonic projections to the dentate gyrus of the rat."}
{"id": "PMID:460701", "title": "The site of origin of cardiac preganglionic fibers of the vagus nerve: an HRP study in the cat.", "content": "After injection of horseradish peroxidase (HRP) into the right cardiac branches of the vagus nerve in the cat, the majority of HRP-labeled neurons were located ipsilaterally in the reticular formation ventrolateral to the nucleus ambiguus. Additionally, HRP-labeled neurons were also observed within the nucleus ambiguus (Am) and the dorsal motor nucleus of the vagus nerve (DM).", "contents": "The site of origin of cardiac preganglionic fibers of the vagus nerve: an HRP study in the cat. After injection of horseradish peroxidase (HRP) into the right cardiac branches of the vagus nerve in the cat, the majority of HRP-labeled neurons were located ipsilaterally in the reticular formation ventrolateral to the nucleus ambiguus. Additionally, HRP-labeled neurons were also observed within the nucleus ambiguus (Am) and the dorsal motor nucleus of the vagus nerve (DM)."}
{"id": "PMID:460702", "title": "Convergent prefrontal and nigral projections to the striatum of the rat.", "content": "Projections to the striatum from the prefrontal cortex, the substantia nigra's pars compacta (SNC), and the ventral tegmental area (AVT) were examined autoradiographically. These projections converge on the striatum in an organized manner such that the prefrontal areas innervated by AVT project to the same part of the striatum as AVT, and prefrontal areas innervated by SNC project to the same part of the striatum as SNC.", "contents": "Convergent prefrontal and nigral projections to the striatum of the rat. Projections to the striatum from the prefrontal cortex, the substantia nigra's pars compacta (SNC), and the ventral tegmental area (AVT) were examined autoradiographically. These projections converge on the striatum in an organized manner such that the prefrontal areas innervated by AVT project to the same part of the striatum as AVT, and prefrontal areas innervated by SNC project to the same part of the striatum as SNC."}
{"id": "PMID:460703", "title": "The GABA agonist THIP a muscimol analogue, does not interfere with the benzodiazepine binding site on rats cortical membranes.", "content": "THIP, a cyclic analogue of muscimol, is a powerful GABA agonist. It is as active as GABA in displacing [3H]muscimol from its binding site to cerebellar membranes (IC50 = 31.5 +/- 2.5 mM). However, unlike muscimol or GABA, it is devoid of any modulatory interaction with the benzodiazepine binding site on rat's cortical membranes. Homotaurine, isoguvacine and imidazole acetic acid are less active than muscimol and GABA for increasing the affinity of [3H]diazepam to cortical membrane preparations.", "contents": "The GABA agonist THIP a muscimol analogue, does not interfere with the benzodiazepine binding site on rats cortical membranes. THIP, a cyclic analogue of muscimol, is a powerful GABA agonist. It is as active as GABA in displacing [3H]muscimol from its binding site to cerebellar membranes (IC50 = 31.5 +/- 2.5 mM). However, unlike muscimol or GABA, it is devoid of any modulatory interaction with the benzodiazepine binding site on rat's cortical membranes. Homotaurine, isoguvacine and imidazole acetic acid are less active than muscimol and GABA for increasing the affinity of [3H]diazepam to cortical membrane preparations."}
{"id": "PMID:460704", "title": "Plasticity of spinovestibular projections in response to hemicerebellectomy in newborn rats.", "content": "Spinal cord projections to the lateral vestibular nucleus (LVN) are relatively sparse in normal adult black-hooded rats. However, in response to hemicerebellectomy in 2--3-day-old rats an increase in spinovestibular fibers to the ipsilateral lateral vestibular nucleus was observed. This increased input appears to be due to fibers which would normally be destined for the cerebellum but which alternatively project to the functionally related lateral vestibular nucleus. Considering the neuroanatomical relationships between the cerebellum and the vestibular nuclei and in view of the functional compensation frequently reported after cerebellar lesions, the observed remodelling may represent a partial anatomical basis for recovery of function.", "contents": "Plasticity of spinovestibular projections in response to hemicerebellectomy in newborn rats. Spinal cord projections to the lateral vestibular nucleus (LVN) are relatively sparse in normal adult black-hooded rats. However, in response to hemicerebellectomy in 2--3-day-old rats an increase in spinovestibular fibers to the ipsilateral lateral vestibular nucleus was observed. This increased input appears to be due to fibers which would normally be destined for the cerebellum but which alternatively project to the functionally related lateral vestibular nucleus. Considering the neuroanatomical relationships between the cerebellum and the vestibular nuclei and in view of the functional compensation frequently reported after cerebellar lesions, the observed remodelling may represent a partial anatomical basis for recovery of function."}
{"id": "PMID:460705", "title": "A non-adrenergic nerve ending containing small granular vesicles in the guinea-pig gut.", "content": "Two distinct types of small granular vesicle(SGV)-containing nerve ending are described in the gastrointestinal tract of the guinea-pig. One of these types of ending accumulates 5- and 6-hydroxydopamine (OHDA) and is therefore adrenergic. The other type remains unchanged after in vivo exposure to these drugs. The widespread distribution of this nerve in the smooth muscle layers of the large and small bowel suggests that it may play a role in gut motility.", "contents": "A non-adrenergic nerve ending containing small granular vesicles in the guinea-pig gut. Two distinct types of small granular vesicle(SGV)-containing nerve ending are described in the gastrointestinal tract of the guinea-pig. One of these types of ending accumulates 5- and 6-hydroxydopamine (OHDA) and is therefore adrenergic. The other type remains unchanged after in vivo exposure to these drugs. The widespread distribution of this nerve in the smooth muscle layers of the large and small bowel suggests that it may play a role in gut motility."}
{"id": "PMID:460706", "title": "External and internal effects of tetraethylammonium on voltage-dependent and Ca-dependent K+ currents components in molluscan pacemaker neurons.", "content": "Ca-dependent and voltage-dependent K+ currents in Aplysia pacemaker neurons were studied separately by electrophoretic injections of Ca2+ ions into the cells to induce the Ca-dependent component and by step depolarizations in the absence of external Ca2+ ions to induce the voltage-dependent component. Intracellular tetraethylammonium (TEA) blocks the voltage-dependent but not the Ca-dependent K+ current. Extracellular TEA blocks both components reversibly but is more effective in inhibiting the Ca-dependent K+ currents. Our results suggest that there are TEA receptor sites for the voltage-dependent K+ current on each side of the membrane, but only an external receptor site for the Ca-dependent K+ current.", "contents": "External and internal effects of tetraethylammonium on voltage-dependent and Ca-dependent K+ currents components in molluscan pacemaker neurons. Ca-dependent and voltage-dependent K+ currents in Aplysia pacemaker neurons were studied separately by electrophoretic injections of Ca2+ ions into the cells to induce the Ca-dependent component and by step depolarizations in the absence of external Ca2+ ions to induce the voltage-dependent component. Intracellular tetraethylammonium (TEA) blocks the voltage-dependent but not the Ca-dependent K+ current. Extracellular TEA blocks both components reversibly but is more effective in inhibiting the Ca-dependent K+ currents. Our results suggest that there are TEA receptor sites for the voltage-dependent K+ current on each side of the membrane, but only an external receptor site for the Ca-dependent K+ current."}
{"id": "PMID:460707", "title": "The comparative effectiveness of the \"brown and blue reactions\" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase.", "content": "The comparative advantages and disadvantages of diaminobenzidine (DAB) and benzidine di--HCl (BDHC) procedures for tracing processes of cells injected intracellularly with horseradish peroxidase (HRP) have been studied. The DAB reaction gives a good definition of membranes and appears more suitable for visualizing the fine structure of dendrites. The BDHC procedure, being more sensitive, induces crystal formation and heavily filled processes appear frosted. This last procedure is, however, much more appropriate for tracing the axonal system of injected cells.", "contents": "The comparative effectiveness of the \"brown and blue reactions\" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase. The comparative advantages and disadvantages of diaminobenzidine (DAB) and benzidine di--HCl (BDHC) procedures for tracing processes of cells injected intracellularly with horseradish peroxidase (HRP) have been studied. The DAB reaction gives a good definition of membranes and appears more suitable for visualizing the fine structure of dendrites. The BDHC procedure, being more sensitive, induces crystal formation and heavily filled processes appear frosted. This last procedure is, however, much more appropriate for tracing the axonal system of injected cells."}
{"id": "PMID:460708", "title": "Electrophysiology of the guinea-pig pineal organ: sympathetically influenced cells responding differently to light and darkness.", "content": "Single cell recordings (extracellular) from the posterior part of the guinea-pig pineal organ reveals two groups of sympathetically influenced spontaneously active cells: one group is stimulated by light and inhibited by darkness; the other type of cell whose activity was monitored over a period of 15 h shows activation by darkness and diminished activity after the onset of light. It is suggested that latter type is involved in melatonin synthesis.", "contents": "Electrophysiology of the guinea-pig pineal organ: sympathetically influenced cells responding differently to light and darkness. Single cell recordings (extracellular) from the posterior part of the guinea-pig pineal organ reveals two groups of sympathetically influenced spontaneously active cells: one group is stimulated by light and inhibited by darkness; the other type of cell whose activity was monitored over a period of 15 h shows activation by darkness and diminished activity after the onset of light. It is suggested that latter type is involved in melatonin synthesis."}
{"id": "PMID:460709", "title": "Small, intensely fluorescent cells of human sympathetic ganglia.", "content": "Human sympathetic ganglia obtained from sympathectomies were studied using the formaldehyde-induced fluorescence (FIF) method and electron microscopy to identify the small, intensely fluorescent (Sif) cells. Microspectrofluorimetry was used to study the intensity of the emitted FIF and the emission spectra. The SIF cells were found in every ganglion studied, but they were very sparce. They occurred alone or in small clusters and were mostly situated close to the blood vessels. The SIF cells contained high amounts of catecholamines, as indicated by microspectrofluorimetry. Electron microscopy revealed granule-containing cells (GC) enveloped by satellite cells; the former cells contained osmiophilic granules 300--400 nm in diameter and synapses of the cholinergic type were frequently found on their surface.", "contents": "Small, intensely fluorescent cells of human sympathetic ganglia. Human sympathetic ganglia obtained from sympathectomies were studied using the formaldehyde-induced fluorescence (FIF) method and electron microscopy to identify the small, intensely fluorescent (Sif) cells. Microspectrofluorimetry was used to study the intensity of the emitted FIF and the emission spectra. The SIF cells were found in every ganglion studied, but they were very sparce. They occurred alone or in small clusters and were mostly situated close to the blood vessels. The SIF cells contained high amounts of catecholamines, as indicated by microspectrofluorimetry. Electron microscopy revealed granule-containing cells (GC) enveloped by satellite cells; the former cells contained osmiophilic granules 300--400 nm in diameter and synapses of the cholinergic type were frequently found on their surface."}
{"id": "PMID:460710", "title": "Projections to the ventral surface of the cat brainstem demonstrated by horseradish peroxidase.", "content": "Topical application of horseradish peroxidase (HRP) to the ventral surface of the medulla oblongata revealed various cells with axons terminating near this region. Fibers traced from the ventral surface originated in the nucleus of the tractus solitarius and the dorsal nucleus of the vagus, both near the dorsal surface of the medulla. Projections from the cochlear nucleus were also observed. Using short HRP contact time, fibers were shown which terminate at, or near, the ventral surface. The possible involvement of these projections in various responses mediated from the ventral surface is discussed.", "contents": "Projections to the ventral surface of the cat brainstem demonstrated by horseradish peroxidase. Topical application of horseradish peroxidase (HRP) to the ventral surface of the medulla oblongata revealed various cells with axons terminating near this region. Fibers traced from the ventral surface originated in the nucleus of the tractus solitarius and the dorsal nucleus of the vagus, both near the dorsal surface of the medulla. Projections from the cochlear nucleus were also observed. Using short HRP contact time, fibers were shown which terminate at, or near, the ventral surface. The possible involvement of these projections in various responses mediated from the ventral surface is discussed."}
{"id": "PMID:460711", "title": "Localization of Leu-enkephalin in dense core vesicles of axon terminals.", "content": "The localization of enkephalins by immunofluorescence has already shown that these pentapeptides are largely distributed in the central nervous system. In order to clearly identify the structures and organelles containing enkephalins, an immunohistochemical localization of Leu-enkephalin was performed at the ultrastructural level. Leu-enkephalin was detected in dense core vesicles (70--100 nm in diameter) of many nerve fibers in the substantia gelatinosa of the spinal cord, the globus pallidus and the nucleus papabrachialis. These findings support the hypothesis that Leu-enkephalin could act as a neuromodulator.", "contents": "Localization of Leu-enkephalin in dense core vesicles of axon terminals. The localization of enkephalins by immunofluorescence has already shown that these pentapeptides are largely distributed in the central nervous system. In order to clearly identify the structures and organelles containing enkephalins, an immunohistochemical localization of Leu-enkephalin was performed at the ultrastructural level. Leu-enkephalin was detected in dense core vesicles (70--100 nm in diameter) of many nerve fibers in the substantia gelatinosa of the spinal cord, the globus pallidus and the nucleus papabrachialis. These findings support the hypothesis that Leu-enkephalin could act as a neuromodulator."}
{"id": "PMID:460712", "title": "Somatosensory cortex: acetylcholinesterase staining of barrel neuropil in the rat.", "content": "Acetylcholinesterase (AchE) staining of layer IV of rat somatosensory (SmI) cortex was studied. In the barrel field of SmI, there are periodic, intensely AchE staining foci in the pattern and with the dimensions of barrels. The onset of this staining, at age 3 days, corresponds with the arrival of thalamocortical input to the barrels. Undercutting of SmI prevents staining in layer IV. We conclude that there are AchE-rich zones in layer IV that coincide with the specific thalamocortical projection to SmI.", "contents": "Somatosensory cortex: acetylcholinesterase staining of barrel neuropil in the rat. Acetylcholinesterase (AchE) staining of layer IV of rat somatosensory (SmI) cortex was studied. In the barrel field of SmI, there are periodic, intensely AchE staining foci in the pattern and with the dimensions of barrels. The onset of this staining, at age 3 days, corresponds with the arrival of thalamocortical input to the barrels. Undercutting of SmI prevents staining in layer IV. We conclude that there are AchE-rich zones in layer IV that coincide with the specific thalamocortical projection to SmI."}
{"id": "PMID:460713", "title": "Self-stimulation of the sulcal prefrontal cortex in the rat: direct evidence for ascending dopaminergic mediation.", "content": "Intracranial self-stimulation (ICSS) of the prefrontal cortex dorsal to the rhinal sulcus in rats has been abolished by means of injections of 6-hydroxydopamine (6-OHDA) (4 micrograms/2 microliters) into the ascending trajectory of the A10 mesocortical dopaminergic fibers ipsilateral to the stimulation electrodes. Similar injections made contralateral to the stimulation electrodes produced a transient attenuation of this self-stimulation. All cases of such lesions were confirmed with the use of Vibratome histochemical fluorescence examination of each of the dopamine projection areas in the forebrain.", "contents": "Self-stimulation of the sulcal prefrontal cortex in the rat: direct evidence for ascending dopaminergic mediation. Intracranial self-stimulation (ICSS) of the prefrontal cortex dorsal to the rhinal sulcus in rats has been abolished by means of injections of 6-hydroxydopamine (6-OHDA) (4 micrograms/2 microliters) into the ascending trajectory of the A10 mesocortical dopaminergic fibers ipsilateral to the stimulation electrodes. Similar injections made contralateral to the stimulation electrodes produced a transient attenuation of this self-stimulation. All cases of such lesions were confirmed with the use of Vibratome histochemical fluorescence examination of each of the dopamine projection areas in the forebrain."}
{"id": "PMID:460714", "title": "Convergence of sensory input from tooth pulp, optic chiasm and sciatic nerve onto locus coeruleus neurons in the rat.", "content": "Electrophysiological studies using rats were performed to determine whether or not neurons in the locus coeruleus (LC), subcoeruleus and parabrachial areas receive convergence of sensory input from tooth pulp (TP), optic chiasm (OC) and sciatic nerve (SN). Convergence of input from these nerves was observed in 23% and 33% of LC and subcoeruleus neurons, respectively, and from two nerves in 31% and 20% of LC and subcoeruleus neurons, respectively, while convergence was rarely detected in parabranchial neurons. These results suggest that there is a convergence of peripheral sensory input onto more than half the noradrenergic coerulear neurons.", "contents": "Convergence of sensory input from tooth pulp, optic chiasm and sciatic nerve onto locus coeruleus neurons in the rat. Electrophysiological studies using rats were performed to determine whether or not neurons in the locus coeruleus (LC), subcoeruleus and parabrachial areas receive convergence of sensory input from tooth pulp (TP), optic chiasm (OC) and sciatic nerve (SN). Convergence of input from these nerves was observed in 23% and 33% of LC and subcoeruleus neurons, respectively, and from two nerves in 31% and 20% of LC and subcoeruleus neurons, respectively, while convergence was rarely detected in parabranchial neurons. These results suggest that there is a convergence of peripheral sensory input onto more than half the noradrenergic coerulear neurons."}
{"id": "PMID:460715", "title": "Interactions between spinocervicolemniscal and dorsal column pathways in thalamic ventrobasal neurons in the cat.", "content": "Ventrobasal (VB) thalamic cells responding to electrical stimulation of the dorsal column (DC) and of the dorsolateral funiculus DLF in cats with DC intact or cut at C4 and isolated, were studied. Among the cells observed, about 30% showed convergence to the two pathways. Different patterns of interaction between the two inputs were found, but the most frequent effect observed was an inhibition exerted by dorsal column (conditioning) stimulation on the activity evoked by dorsolateral funiculi (test) stimulation. From these results a dominance of the DC over the spinocervico-thalamic (SCT) inputs was confirmed.", "contents": "Interactions between spinocervicolemniscal and dorsal column pathways in thalamic ventrobasal neurons in the cat. Ventrobasal (VB) thalamic cells responding to electrical stimulation of the dorsal column (DC) and of the dorsolateral funiculus DLF in cats with DC intact or cut at C4 and isolated, were studied. Among the cells observed, about 30% showed convergence to the two pathways. Different patterns of interaction between the two inputs were found, but the most frequent effect observed was an inhibition exerted by dorsal column (conditioning) stimulation on the activity evoked by dorsolateral funiculi (test) stimulation. From these results a dominance of the DC over the spinocervico-thalamic (SCT) inputs was confirmed."}
{"id": "PMID:460716", "title": "In vitro radioisotope precursor incorporation into ribonucleic acid and protein in dystrophic mouse gastrocnemius muscle (C57 BL/6J dy2J/dy2J).", "content": "An in vitro tissue slice technique has been developed and used to compare radioisotope precurosr incorporation into RNA and protein in normal and dystrophic mouse gastrophic muscle. Significant differences are observed with both RNA and protein when incorporation is measured on a fresh weight of muscle basis. Specific activity comparisons show significantly increased incorporation with protein but not with RNA. A comparison of the results with in vivo studies has been made. The in vitro system developed is applicable to the study of macromolecular metabolism in normal and diseased human muscle tissue.", "contents": "In vitro radioisotope precursor incorporation into ribonucleic acid and protein in dystrophic mouse gastrocnemius muscle (C57 BL/6J dy2J/dy2J). An in vitro tissue slice technique has been developed and used to compare radioisotope precurosr incorporation into RNA and protein in normal and dystrophic mouse gastrophic muscle. Significant differences are observed with both RNA and protein when incorporation is measured on a fresh weight of muscle basis. Specific activity comparisons show significantly increased incorporation with protein but not with RNA. A comparison of the results with in vivo studies has been made. The in vitro system developed is applicable to the study of macromolecular metabolism in normal and diseased human muscle tissue."}
{"id": "PMID:460717", "title": "On the presence of extraneuronal catecholamine in the iris of the rat: a scanning microfluorimetric study.", "content": "Formaldehyde-induced fluorescence (FIF) of noradrenalin (NA) was studied in histochemical preparations of the rat iris. With the aid of a two-dimensional microfluorimetric scanning method, quantitative information was obtained from neuronal and non-neuronal elements selectively. Spectral analysis and fluorimetric studies showed the existence of extraneuronal NA in the iris of untreated rats. Surgical and pharmacological (reserpine, amphetamine, imipramine, nialamide) manipulation of sympathetic neurotransmission-induced changes in extraneuronal fluorescence, which did not parallel changes in fluorescence of neuronal elements. A positive relationship was found between changes in extraneuronal fluorescence and the postsynaptic effect (pupil diameter). We hypothesize that extraneuronal catecholamine (CA) fluorescence is a histochemical parameter for synaptic neurotransmission.", "contents": "On the presence of extraneuronal catecholamine in the iris of the rat: a scanning microfluorimetric study. Formaldehyde-induced fluorescence (FIF) of noradrenalin (NA) was studied in histochemical preparations of the rat iris. With the aid of a two-dimensional microfluorimetric scanning method, quantitative information was obtained from neuronal and non-neuronal elements selectively. Spectral analysis and fluorimetric studies showed the existence of extraneuronal NA in the iris of untreated rats. Surgical and pharmacological (reserpine, amphetamine, imipramine, nialamide) manipulation of sympathetic neurotransmission-induced changes in extraneuronal fluorescence, which did not parallel changes in fluorescence of neuronal elements. A positive relationship was found between changes in extraneuronal fluorescence and the postsynaptic effect (pupil diameter). We hypothesize that extraneuronal catecholamine (CA) fluorescence is a histochemical parameter for synaptic neurotransmission."}
{"id": "PMID:460718", "title": "Olivo-cerebellar activity during harmaline-induced tremor.A 2-[14C]deoxyglucose study.", "content": "The specific neuronal activity of the olivo-cerebellar system of the cat under the effect of harmaline has been studied using the 2-[14C]deoxyglucose method. The results obtained in animals treated with tremogenic doses of harmaline, but immobilized with flaxedil, have been compared with those of non-treated controls. An increased radioactivity in specific regions of the olivo-cerebellar system including parts of the medial and dorsal accessory olive and the corresponding receiving molecular layer have been found. No involvement of the granule cell layer nor of the nuclear cells has been observed. It is therefore suggested that the tremogenic rhythmicity of the cerebellar output is organized uniquely by an increased climbing fiber input.", "contents": "Olivo-cerebellar activity during harmaline-induced tremor.A 2-[14C]deoxyglucose study. The specific neuronal activity of the olivo-cerebellar system of the cat under the effect of harmaline has been studied using the 2-[14C]deoxyglucose method. The results obtained in animals treated with tremogenic doses of harmaline, but immobilized with flaxedil, have been compared with those of non-treated controls. An increased radioactivity in specific regions of the olivo-cerebellar system including parts of the medial and dorsal accessory olive and the corresponding receiving molecular layer have been found. No involvement of the granule cell layer nor of the nuclear cells has been observed. It is therefore suggested that the tremogenic rhythmicity of the cerebellar output is organized uniquely by an increased climbing fiber input."}
{"id": "PMID:460719", "title": "Evidence for a multiple innervation of subcommissural ependymocytes in the rat.", "content": "Glandular ependymocytes of the rat subcommissural organ (SCO) receive a multiple innervation: a serotonergic afference identified by radioautography and at least one other input of a hitherto unknown transmitter content. Both types of terminals make well differentiated axo-glandular synapses with SCO-ependymocytes, but display different morphological characteristics. Serotonergic terminals being considered as inhibitory, it is suggested that the other type of terminal is excitatory.", "contents": "Evidence for a multiple innervation of subcommissural ependymocytes in the rat. Glandular ependymocytes of the rat subcommissural organ (SCO) receive a multiple innervation: a serotonergic afference identified by radioautography and at least one other input of a hitherto unknown transmitter content. Both types of terminals make well differentiated axo-glandular synapses with SCO-ependymocytes, but display different morphological characteristics. Serotonergic terminals being considered as inhibitory, it is suggested that the other type of terminal is excitatory."}
{"id": "PMID:460720", "title": "Radioimmunologic localization of vasoactive intestinal polypeptide in hypothalamic and extrahypothalamic sites in the rat brain.", "content": "Using a specific radioimmunoassay (RIA), we have quantitated the hypothalamic and extrahypothalamic distribution of vasoactive intestinal polypeptide (VIP) in the rat brain. Highest concentrations of VIP were found in cerebral cortex, suprachiasmatic nucleus, and medial amygdaloid nucleus. Lower concentrations were found in the anterior hypothalamic area, medial preoptic area, peri- and paraventricular nuclei, and hippocampus. Significant levels of VIP were also found in the anterior pituitary. Very little VIP was detected in median eminence and cerebellar cortex. This distribution of VIP in areas of the rat brain known to be important to neuroendocrine function suggests a role for VIP in the control of anterior pituitary events.", "contents": "Radioimmunologic localization of vasoactive intestinal polypeptide in hypothalamic and extrahypothalamic sites in the rat brain. Using a specific radioimmunoassay (RIA), we have quantitated the hypothalamic and extrahypothalamic distribution of vasoactive intestinal polypeptide (VIP) in the rat brain. Highest concentrations of VIP were found in cerebral cortex, suprachiasmatic nucleus, and medial amygdaloid nucleus. Lower concentrations were found in the anterior hypothalamic area, medial preoptic area, peri- and paraventricular nuclei, and hippocampus. Significant levels of VIP were also found in the anterior pituitary. Very little VIP was detected in median eminence and cerebellar cortex. This distribution of VIP in areas of the rat brain known to be important to neuroendocrine function suggests a role for VIP in the control of anterior pituitary events."}
{"id": "PMID:460721", "title": "Experimental modification of interlimb coordination during locomotion of a Crusacea.", "content": "An anlaysis of the phase relationship between the same side legs (homolateral coupling) of Rock Lobster (Crustacea) is performed during sequence of locomtion. On intact animals the phase relationship is similar to the most common insect or mammalian patterns ('alternating model'). After successive autotomy of the walking legs, another coordination pattern occurs progressively; it is an 'in phase model'. From these experiments it can be concluded that there exists a central motor program tightly coordinating each thoracic ganglion and that the alternating pattern could be due to a reorganization of the synchronous system by a peripheral proprioceptive inflow.", "contents": "Experimental modification of interlimb coordination during locomotion of a Crusacea. An anlaysis of the phase relationship between the same side legs (homolateral coupling) of Rock Lobster (Crustacea) is performed during sequence of locomtion. On intact animals the phase relationship is similar to the most common insect or mammalian patterns ('alternating model'). After successive autotomy of the walking legs, another coordination pattern occurs progressively; it is an 'in phase model'. From these experiments it can be concluded that there exists a central motor program tightly coordinating each thoracic ganglion and that the alternating pattern could be due to a reorganization of the synchronous system by a peripheral proprioceptive inflow."}
{"id": "PMID:460722", "title": "Intrinsic excitation in the rat neostriatum mediated by acetylcholine.", "content": "Synaptic excitation elicited by local stimulation in neostriatal slices was found to be mediated by acetylcholine (ACh). The synapses generating this excitation belong to intrinsic neurons. Thus, for the first time, direct evidence for the existence of intrinsic excitatory cholinergic neurons in the neostriatum is provided.", "contents": "Intrinsic excitation in the rat neostriatum mediated by acetylcholine. Synaptic excitation elicited by local stimulation in neostriatal slices was found to be mediated by acetylcholine (ACh). The synapses generating this excitation belong to intrinsic neurons. Thus, for the first time, direct evidence for the existence of intrinsic excitatory cholinergic neurons in the neostriatum is provided."}
{"id": "PMID:460723", "title": "Experimental modification of cerebellar development in tissue culture: x-irradiation induces granular degeneration and unattached purkinje cell dendritic spines.", "content": "Cerebellum explants from 15--16 day fetal mice were irradiated with 500--1000 rad X-ray and were grown in tissue culture for 12--18 days. At the dose of 500 rad, a reduction of granule cell population was observed in the explant. At the dose of 1000 rad, granule cells were mostly destroyed leaving Purkinje cells disarrayed and clustered. Electron microscopy of the explants revealed the presence of unattached dendritic spines of Purkinje cell. It was noted that although numerous free spines could be seen in X-irradiated cultures, the postsynaptic specialization of these spines occurred only in ones embedded in astrocytic cytoplasm. The results indicate that (a) the formation of dendritic spines is a property of the Purkinje cell which probably is preprogrammed and (b) the postsynaptic differentiation of the unattached spines is directly related to their contacts with astrocytes.", "contents": "Experimental modification of cerebellar development in tissue culture: x-irradiation induces granular degeneration and unattached purkinje cell dendritic spines. Cerebellum explants from 15--16 day fetal mice were irradiated with 500--1000 rad X-ray and were grown in tissue culture for 12--18 days. At the dose of 500 rad, a reduction of granule cell population was observed in the explant. At the dose of 1000 rad, granule cells were mostly destroyed leaving Purkinje cells disarrayed and clustered. Electron microscopy of the explants revealed the presence of unattached dendritic spines of Purkinje cell. It was noted that although numerous free spines could be seen in X-irradiated cultures, the postsynaptic specialization of these spines occurred only in ones embedded in astrocytic cytoplasm. The results indicate that (a) the formation of dendritic spines is a property of the Purkinje cell which probably is preprogrammed and (b) the postsynaptic differentiation of the unattached spines is directly related to their contacts with astrocytes."}
{"id": "PMID:460724", "title": "Lingual projections to the dorsal pons of the rat, studied by the field potential analysis.", "content": "Electrical stimulation of the chorda tympani (CT) of a rat produced a negative field potential with a peak latency of 8 msec localized in the parabrachial nucleus (PB) which may correspond to the pontine taste area. In contrast, stimulation of the glossopharyngeal nerve (GL) gave rise to no detectable negative field potential around the brachium conjunctivum. Stimulation of the lingual nerve (L) produced a large negative field potential with a similar latency, whose focus was found located just lateral to the focus of the negative potentials produced by CT stimulation.", "contents": "Lingual projections to the dorsal pons of the rat, studied by the field potential analysis. Electrical stimulation of the chorda tympani (CT) of a rat produced a negative field potential with a peak latency of 8 msec localized in the parabrachial nucleus (PB) which may correspond to the pontine taste area. In contrast, stimulation of the glossopharyngeal nerve (GL) gave rise to no detectable negative field potential around the brachium conjunctivum. Stimulation of the lingual nerve (L) produced a large negative field potential with a similar latency, whose focus was found located just lateral to the focus of the negative potentials produced by CT stimulation."}
{"id": "PMID:460725", "title": "Development of vestibulo-ocular response in the kitten.", "content": "Vestibulo-ocular response (VOR) has been recorded in kittens aged between 10 and 61 postnatal days. Responses to velocity steps and to sinusoidal oscillations are present on the earliest recordings, though they are limited to a low gain slow compensatory gaze displacement. Vestibular-induced saccades appear only around postnatal day 30, but adult-like responses with a high gain are not displayed before the end of the second month. Optokinetic nystagmus (OKN) appears earlier (around postnatal day 25) and maturates faster.", "contents": "Development of vestibulo-ocular response in the kitten. Vestibulo-ocular response (VOR) has been recorded in kittens aged between 10 and 61 postnatal days. Responses to velocity steps and to sinusoidal oscillations are present on the earliest recordings, though they are limited to a low gain slow compensatory gaze displacement. Vestibular-induced saccades appear only around postnatal day 30, but adult-like responses with a high gain are not displayed before the end of the second month. Optokinetic nystagmus (OKN) appears earlier (around postnatal day 25) and maturates faster."}
{"id": "PMID:460726", "title": "Lack of effect by substance P at sites in the substantia gelatinosa where Met-enkephalin reduces the transmission of nociceptive impulses.", "content": "In alpha-chloralose anaesthetized spinal cats, Met-enkephalin amide (M-ENKA) and substance P were administered electrophoretically in the substantia gelatinosa while studying the excitation of deeper spinal neurones by noxious and non-noxious cutaneous stimuli. At sites where Met-enkephalin selectively reduced excitation by noxious skin stimuli, substance P was without effect. This result does not support the hypothesis that enkephalins are released at axo-axonic synapses on the terminals of substance P releasing primary afferent fibres.", "contents": "Lack of effect by substance P at sites in the substantia gelatinosa where Met-enkephalin reduces the transmission of nociceptive impulses. In alpha-chloralose anaesthetized spinal cats, Met-enkephalin amide (M-ENKA) and substance P were administered electrophoretically in the substantia gelatinosa while studying the excitation of deeper spinal neurones by noxious and non-noxious cutaneous stimuli. At sites where Met-enkephalin selectively reduced excitation by noxious skin stimuli, substance P was without effect. This result does not support the hypothesis that enkephalins are released at axo-axonic synapses on the terminals of substance P releasing primary afferent fibres."}
{"id": "PMID:460727", "title": "Enkephalin levels in rat brain after various regimens of morphine administration.", "content": "Rats implanted with a single morphine pellet for a short period of 72 h have been shown to have a lower concentration of enkephalin in the brain, as determined by a radioimmunoassay (RIA) detecting Leu- and Met-enkephalin as well as another endogenous substance. Two other paradigms of morphine implantations did not cause any change in enkephalin levels. Various protocols of morphine injections failed to elicit changes in enkephalin content in the rat brain. The significant decrease observed after the short regimen implantation suggests that at a certain stage in the development of physical dependence a reduction in the amount of enkephalin occurs, but returns to normal values within a short period.", "contents": "Enkephalin levels in rat brain after various regimens of morphine administration. Rats implanted with a single morphine pellet for a short period of 72 h have been shown to have a lower concentration of enkephalin in the brain, as determined by a radioimmunoassay (RIA) detecting Leu- and Met-enkephalin as well as another endogenous substance. Two other paradigms of morphine implantations did not cause any change in enkephalin levels. Various protocols of morphine injections failed to elicit changes in enkephalin content in the rat brain. The significant decrease observed after the short regimen implantation suggests that at a certain stage in the development of physical dependence a reduction in the amount of enkephalin occurs, but returns to normal values within a short period."}
{"id": "PMID:460728", "title": "Turning behaviour induced by injection of muscimol or picrotoxin into the substantia nigra demonstrates dual GABA components.", "content": "Injection of the GABA agonist muscimol into rat caudal substantia nigra caused contralateral turning, whereas injection into the rostral substantia nigra caused ipsilateral turning. The GABA antagonist picrotoxin had the opposite effect. These findings support the hypothesis that GABA has dual actions in the substantia nigra. Ipsilateral turning induced by injection of muscimol into rostral nigra was abolished by haloperidol pretreatment, indicating the involvement of dopaminergic mechanisms. Haloperidol pre-treatment did not prevent turning induced by muscimol injected into the caudal nigra, supporting the existence of a non-dopaminergic nigral output system.", "contents": "Turning behaviour induced by injection of muscimol or picrotoxin into the substantia nigra demonstrates dual GABA components. Injection of the GABA agonist muscimol into rat caudal substantia nigra caused contralateral turning, whereas injection into the rostral substantia nigra caused ipsilateral turning. The GABA antagonist picrotoxin had the opposite effect. These findings support the hypothesis that GABA has dual actions in the substantia nigra. Ipsilateral turning induced by injection of muscimol into rostral nigra was abolished by haloperidol pretreatment, indicating the involvement of dopaminergic mechanisms. Haloperidol pre-treatment did not prevent turning induced by muscimol injected into the caudal nigra, supporting the existence of a non-dopaminergic nigral output system."}
{"id": "PMID:460729", "title": "Effects of picrotoxin and strychnine on fish retinal S-potentials: evidence for inhibitory control of depolarizing responses.", "content": "Simultaneous recording of the light evoked electrical signals from different classes of slow(S)-potential unit in the isolated fish retina established that picrotoxin, an antagonist of GABA, selectively suppresses the depolarizing component of C-type S-potentials. In contrast, equivalent concentrations of strychnine have no effect on S-potentials, but suppress a component of transient amacrine cell responses. On the basis of these and other experimental data [3,4] we propose a network diagram for the C-type S-potential units in the cyprinid fish retina. This network includes a GABA-ergic feed-back loop and is essentially similar to that proposed by Fuortes and Simon [5].", "contents": "Effects of picrotoxin and strychnine on fish retinal S-potentials: evidence for inhibitory control of depolarizing responses. Simultaneous recording of the light evoked electrical signals from different classes of slow(S)-potential unit in the isolated fish retina established that picrotoxin, an antagonist of GABA, selectively suppresses the depolarizing component of C-type S-potentials. In contrast, equivalent concentrations of strychnine have no effect on S-potentials, but suppress a component of transient amacrine cell responses. On the basis of these and other experimental data [3,4] we propose a network diagram for the C-type S-potential units in the cyprinid fish retina. This network includes a GABA-ergic feed-back loop and is essentially similar to that proposed by Fuortes and Simon [5]."}
{"id": "PMID:460730", "title": "The inhibitory action of glycine on rat cortical neurons.", "content": "The action of glycine (Gly) on rat cortical neurons was studied. Gly, iontophoretically applied, hyperpolarized the membrane potential, blocked the firing rate and decreased the amplitude of EPSP-IPSP sequences. Generally the membrane resistance during the aminoacid ejection decreased. The role of Gly in the rat cortical inhibition is discussed.", "contents": "The inhibitory action of glycine on rat cortical neurons. The action of glycine (Gly) on rat cortical neurons was studied. Gly, iontophoretically applied, hyperpolarized the membrane potential, blocked the firing rate and decreased the amplitude of EPSP-IPSP sequences. Generally the membrane resistance during the aminoacid ejection decreased. The role of Gly in the rat cortical inhibition is discussed."}
{"id": "PMID:460731", "title": "Kainic acid: enduring alterations in cerebellar morphology and in cerebral catecholamine and GABA concentrations after cerebellar injection in the rat.", "content": "Kainic acid injected locally in the vermian cortex produces a focal lesion with severe cellular loss. Microscopic changes in 3-week-old preparations are given for both central and peripheral segments. Biochemical studies indicate that norepinephrine, dopamine and gamma-aminobutyric acid (gaba) concentrations in the forebrain are consistently higher on the side of the lesion and remain elevated for at least 3 weeks. It is postulated that disinhibition of cerebellar activity traversing the uncrossed pathway from cerebellar nuclei to catecholamine cell bodies was a major mechanism causing increased catecholamine metabolism in the ipsilateral forebrain.", "contents": "Kainic acid: enduring alterations in cerebellar morphology and in cerebral catecholamine and GABA concentrations after cerebellar injection in the rat. Kainic acid injected locally in the vermian cortex produces a focal lesion with severe cellular loss. Microscopic changes in 3-week-old preparations are given for both central and peripheral segments. Biochemical studies indicate that norepinephrine, dopamine and gamma-aminobutyric acid (gaba) concentrations in the forebrain are consistently higher on the side of the lesion and remain elevated for at least 3 weeks. It is postulated that disinhibition of cerebellar activity traversing the uncrossed pathway from cerebellar nuclei to catecholamine cell bodies was a major mechanism causing increased catecholamine metabolism in the ipsilateral forebrain."}
{"id": "PMID:460732", "title": "Forebrain noradrenaline depletion blocks the release by chlordiazepoxide of behavioral extinction in the rat.", "content": "The interaction between chlordiazepoxide (CDP) and forebrain noradrenaline (NA) was investigated in rats with bilateral radiofrequency lesions of the noradrenergic dorsal tegmental bundle (DTB). Forebrain NA was reduced to 35% of control by the DTB lesions; hypothalamic NA and dopamine were unchanged. Rats were trained on a straight alley runway task for food reward. During extinction of the runway task, CDP significantly increased extinction responding in sham lesion animals but not in DTB lesion animals. Thus, CDP was ineffective in increasing resistance to extinction responding in the runway task in animals with significant depletion of forebrain NA.", "contents": "Forebrain noradrenaline depletion blocks the release by chlordiazepoxide of behavioral extinction in the rat. The interaction between chlordiazepoxide (CDP) and forebrain noradrenaline (NA) was investigated in rats with bilateral radiofrequency lesions of the noradrenergic dorsal tegmental bundle (DTB). Forebrain NA was reduced to 35% of control by the DTB lesions; hypothalamic NA and dopamine were unchanged. Rats were trained on a straight alley runway task for food reward. During extinction of the runway task, CDP significantly increased extinction responding in sham lesion animals but not in DTB lesion animals. Thus, CDP was ineffective in increasing resistance to extinction responding in the runway task in animals with significant depletion of forebrain NA."}
{"id": "PMID:460733", "title": "Cortical lesions modulate turnover rates of acetylcholine and gamma-aminobutyric acid.", "content": "Interruption of the cortico-striatal glutamatergic pathway by decoratication results in significant decreases in the turnover rates of acetylcholine TRACh and gamma-aminobutyric acid TRGABA of striatum. These data support the hypothesis that the cortical input to the striatum is excitatory and acts to modulate cholinergic and GABAergic function in this nucleus. Studies of kainate-induced lesions of the striatum also indicate that most striatal cholinergic interneurons receive a glutamatergic input and that multiple injections of kainate are required to destroy this large population of interneurons.", "contents": "Cortical lesions modulate turnover rates of acetylcholine and gamma-aminobutyric acid. Interruption of the cortico-striatal glutamatergic pathway by decoratication results in significant decreases in the turnover rates of acetylcholine TRACh and gamma-aminobutyric acid TRGABA of striatum. These data support the hypothesis that the cortical input to the striatum is excitatory and acts to modulate cholinergic and GABAergic function in this nucleus. Studies of kainate-induced lesions of the striatum also indicate that most striatal cholinergic interneurons receive a glutamatergic input and that multiple injections of kainate are required to destroy this large population of interneurons."}
{"id": "PMID:460734", "title": "Effects of aldehydes and organic solvents on concanavalin A binding sites in cerebellar tissue sections.", "content": "Fresh frozen cerebellar sections of adult mice treated with aldehydes and organic solvents reveal differences in detectability of Concanavalin A (Con A) binding sites. While fluorescein coupled Con A shows intense labeling of synaptic glomeruli and granule cell bodies under all conditions, the molecular layer labels intensely after treatment with paraformaldehyde, glutaraldehyde, acetone, ethanol and butanol. Complete loss of staining in molecular and granular layers and substantial increase in white matter labeling occurs after chloroform-methanol treatment. Except for glutaraldehyde treated sections, all labeling is specifically inhibited by methyl-alpha-D-mannoside, but not by galactose.", "contents": "Effects of aldehydes and organic solvents on concanavalin A binding sites in cerebellar tissue sections. Fresh frozen cerebellar sections of adult mice treated with aldehydes and organic solvents reveal differences in detectability of Concanavalin A (Con A) binding sites. While fluorescein coupled Con A shows intense labeling of synaptic glomeruli and granule cell bodies under all conditions, the molecular layer labels intensely after treatment with paraformaldehyde, glutaraldehyde, acetone, ethanol and butanol. Complete loss of staining in molecular and granular layers and substantial increase in white matter labeling occurs after chloroform-methanol treatment. Except for glutaraldehyde treated sections, all labeling is specifically inhibited by methyl-alpha-D-mannoside, but not by galactose."}
{"id": "PMID:460735", "title": "An automatic device for forced wakefulness in dogs.", "content": "An automatic device is described which maintains sustained wakefulness of 12 h or more in dogs with chronically implanted electrodes for sleepwaking recording. The procedure consists of automatic sleep detection using EEG, EMG and body movement (BM), and of avoidance conditioning to arouse the animal with sound as conditioned stimulus, and with electroshock as unconditioned stimulus. The procedure does not disturb feeding, drinking and freely moving, and is not associated with forced exercise, or with a marked stress reaction as measured with cortisol and growth hormone. These advantages enable us to maintain 12 h of forced wakefulness in the same animal for 7 or more consecutive days.", "contents": "An automatic device for forced wakefulness in dogs. An automatic device is described which maintains sustained wakefulness of 12 h or more in dogs with chronically implanted electrodes for sleepwaking recording. The procedure consists of automatic sleep detection using EEG, EMG and body movement (BM), and of avoidance conditioning to arouse the animal with sound as conditioned stimulus, and with electroshock as unconditioned stimulus. The procedure does not disturb feeding, drinking and freely moving, and is not associated with forced exercise, or with a marked stress reaction as measured with cortisol and growth hormone. These advantages enable us to maintain 12 h of forced wakefulness in the same animal for 7 or more consecutive days."}
{"id": "PMID:460736", "title": "Early influx of horseradish peroxidase into axons of the hypoglossal nerve during Wallerian degeneration.", "content": "Horseradish peroxidase (HRP) was applied around mouse hypoglossal nerves which were damaged by a crush or a ligature. HRP was then visualized distal to the lesions by light- and electron microscopic histochemistry. At the injury the enzyme entered axons and could also be detected several millimetres down in the distal segment. By 24 h reaction product (r.p.) was either diffusely distributed in the axoplasm or present in various vesicular organelles. Our results indicate that there is a rapid influx of macromolecules into axons after a lesion to a nerve. A similar uptake of 'wound substances' into axons distal to an injury might well have some relation to the process by which axonal breakdown is initiated during Wallerian degeneration.", "contents": "Early influx of horseradish peroxidase into axons of the hypoglossal nerve during Wallerian degeneration. Horseradish peroxidase (HRP) was applied around mouse hypoglossal nerves which were damaged by a crush or a ligature. HRP was then visualized distal to the lesions by light- and electron microscopic histochemistry. At the injury the enzyme entered axons and could also be detected several millimetres down in the distal segment. By 24 h reaction product (r.p.) was either diffusely distributed in the axoplasm or present in various vesicular organelles. Our results indicate that there is a rapid influx of macromolecules into axons after a lesion to a nerve. A similar uptake of 'wound substances' into axons distal to an injury might well have some relation to the process by which axonal breakdown is initiated during Wallerian degeneration."}
{"id": "PMID:460737", "title": "Perivascular nerves in the rat submandibular salivary gland.", "content": "Perivascular nerves in the rat submandibular salivary gland have been studied using a variety of histochemical procedures coupled with electron microscopy. Two principal nerve types, adrenergic and cholinergic, appear to predominate and are localized principally around arterioles. Venules are rarely innervated. The possibility that a non-adrenergic non-cholinergic nerve population might influence blood flow is discussed critically in the light of anatomical and physiological findings.", "contents": "Perivascular nerves in the rat submandibular salivary gland. Perivascular nerves in the rat submandibular salivary gland have been studied using a variety of histochemical procedures coupled with electron microscopy. Two principal nerve types, adrenergic and cholinergic, appear to predominate and are localized principally around arterioles. Venules are rarely innervated. The possibility that a non-adrenergic non-cholinergic nerve population might influence blood flow is discussed critically in the light of anatomical and physiological findings."}
{"id": "PMID:460739", "title": "Contralateral turning after unilateral electrolytic lesion of substantia nigra in thalamic rats.", "content": "Unilateral electrolytic lesions of the substantia nigra of rats induced contralateral turning in intact as well as 'thalamic' rats (devoid of neocortex, hippocampus, striatum, amygdala, septal nuclei, including most of n. accumbens). Therefore, telencephalic structures, including nigrostriatal neurons, are not critically involved in the turning that follows electrolytic nigral lesions.", "contents": "Contralateral turning after unilateral electrolytic lesion of substantia nigra in thalamic rats. Unilateral electrolytic lesions of the substantia nigra of rats induced contralateral turning in intact as well as 'thalamic' rats (devoid of neocortex, hippocampus, striatum, amygdala, septal nuclei, including most of n. accumbens). Therefore, telencephalic structures, including nigrostriatal neurons, are not critically involved in the turning that follows electrolytic nigral lesions."}
{"id": "PMID:460740", "title": "Mitotic spindle fiber orientation in relation to cell migration in the neo-cortex of normal and reeler mouse.", "content": "The angular distribution of mitotic spindle fibers has been studied in the telencephalic ventricular zone (VZ) of mouse embryos and has been found to be monophasic and invariant during the early cortical histogenesis both in the normal and in a reeler embryo. This observation suggests that the orientation of mitotic spindle fibers is independent of nerve cell migration in the embryonic cortex.", "contents": "Mitotic spindle fiber orientation in relation to cell migration in the neo-cortex of normal and reeler mouse. The angular distribution of mitotic spindle fibers has been studied in the telencephalic ventricular zone (VZ) of mouse embryos and has been found to be monophasic and invariant during the early cortical histogenesis both in the normal and in a reeler embryo. This observation suggests that the orientation of mitotic spindle fibers is independent of nerve cell migration in the embryonic cortex."}
{"id": "PMID:460741", "title": "Cat L4-S1 dermatomes determined using signal averaging.", "content": "In view of discrepancies in the earlier literature, a new procedure was devised which used (1) stimuli known to be subthreshold for deep receptor afferents and suprathreshold for hair afferents; (2) averaging techniques applied to dorsal root responses; (3) quantitative mapping of response magnitudes as a function of stimulus location. Dermatomes were obtained for cat L4-S1 dorsal roots which are somewhat larger than the majority of reported whole-root dermatomes, but which are larger than dermatomes approximated from single-unit recordings.", "contents": "Cat L4-S1 dermatomes determined using signal averaging. In view of discrepancies in the earlier literature, a new procedure was devised which used (1) stimuli known to be subthreshold for deep receptor afferents and suprathreshold for hair afferents; (2) averaging techniques applied to dorsal root responses; (3) quantitative mapping of response magnitudes as a function of stimulus location. Dermatomes were obtained for cat L4-S1 dorsal roots which are somewhat larger than the majority of reported whole-root dermatomes, but which are larger than dermatomes approximated from single-unit recordings."}
{"id": "PMID:460742", "title": "Contralateral decrease of neostriatal dopamine concentration after unilateral pallidal lesion in the rat.", "content": "Seven days after unilateral electrolytical and chemical (6-hydroxydopamine (6-OHDA)) lesions in the globus pallidus ipsi- and contralateral neostrial dopamine (DA) concentrations were measured in rats. A definite contralateral DA-decrease was demonstrable, the extent of which was in correlation with ipsilateral decrease.", "contents": "Contralateral decrease of neostriatal dopamine concentration after unilateral pallidal lesion in the rat. Seven days after unilateral electrolytical and chemical (6-hydroxydopamine (6-OHDA)) lesions in the globus pallidus ipsi- and contralateral neostrial dopamine (DA) concentrations were measured in rats. A definite contralateral DA-decrease was demonstrable, the extent of which was in correlation with ipsilateral decrease."}
{"id": "PMID:460744", "title": "Cervix cancer precursors in young offspring of low-income families.", "content": "The revolutionary changes in the mores and practices of adolescent sexuality have not as yet resulted in a significant increase in the rates of cervical cancer precursors in a study of 29,600 young women under age 21. The study represents women from families of low socioeconomic status. Over a 21-year period no cases of carcinoma in situ nor of invasive cervix cancer occurred. The dysplasia rate was low (0.9/1000), and when prerevolutionary and revolutionary periods were compared, there were no significant differences in the rates. Cryocautery was successful in ablating dysplasia, as was follow-up without treatment. An attempt will be made to continue to monitor these young women of the sexual revolution since the effects of their past and current participation might not be discernible for years to come.", "contents": "Cervix cancer precursors in young offspring of low-income families. The revolutionary changes in the mores and practices of adolescent sexuality have not as yet resulted in a significant increase in the rates of cervical cancer precursors in a study of 29,600 young women under age 21. The study represents women from families of low socioeconomic status. Over a 21-year period no cases of carcinoma in situ nor of invasive cervix cancer occurred. The dysplasia rate was low (0.9/1000), and when prerevolutionary and revolutionary periods were compared, there were no significant differences in the rates. Cryocautery was successful in ablating dysplasia, as was follow-up without treatment. An attempt will be made to continue to monitor these young women of the sexual revolution since the effects of their past and current participation might not be discernible for years to come."}
{"id": "PMID:460745", "title": "Wertheim hysterectomy: a reappraisal.", "content": "A total of 610 radical hysterectomies with bilateral pelvic lymphadenectomy were performed at the Mayo Clinic in the 20-year period from 1956 through 1975. This operative procedure was the primary therapy in 74% of the patients, the others having had previous pelvic irradiation. Operative morbidity, postoperative complications, lymph node involvement, and overall survival were analyzed.", "contents": "Wertheim hysterectomy: a reappraisal. A total of 610 radical hysterectomies with bilateral pelvic lymphadenectomy were performed at the Mayo Clinic in the 20-year period from 1956 through 1975. This operative procedure was the primary therapy in 74% of the patients, the others having had previous pelvic irradiation. Operative morbidity, postoperative complications, lymph node involvement, and overall survival were analyzed."}
{"id": "PMID:460746", "title": "Stage I adenocarcinoma of the endometrium. A clinical and histopathological study of 65 cases treated with preoperative radium.", "content": "To determine if preoperative irradiation should be abandoned in favor of post-hysterectomy irradiation, 65 cases of stage I endometrial carcinomas were analyzed. When no residual tumor or no myometrial invasion was found in the irradiated uterus (38 cases), a 5-year cure rate of 97.3% was obtained. When residual tumor or myometrial invasion was found, there was a 5-year cure rate of 100% in grades 1 and 2 (7 cases) but only a 20% cure rate in grade 3 lesions (5 cases). On the basis of these data there appears to be no reason to abandon preoperative irradiation in stage 1 cases. More extensive irradiation and surgery should be considered, however, in grade 3 lesions invading the myometrium.", "contents": "Stage I adenocarcinoma of the endometrium. A clinical and histopathological study of 65 cases treated with preoperative radium. To determine if preoperative irradiation should be abandoned in favor of post-hysterectomy irradiation, 65 cases of stage I endometrial carcinomas were analyzed. When no residual tumor or no myometrial invasion was found in the irradiated uterus (38 cases), a 5-year cure rate of 97.3% was obtained. When residual tumor or myometrial invasion was found, there was a 5-year cure rate of 100% in grades 1 and 2 (7 cases) but only a 20% cure rate in grade 3 lesions (5 cases). On the basis of these data there appears to be no reason to abandon preoperative irradiation in stage 1 cases. More extensive irradiation and surgery should be considered, however, in grade 3 lesions invading the myometrium."}
{"id": "PMID:460747", "title": "The association of parity and marital status with the development of ovarian carcinoma: clinical implications.", "content": "Three hundred twenty-seven patients with ovarian carcinoma were compared with matched controls to determine the association of parity and marital status with the development of ovarian cancer. Nulliparous women were found to have 2.31 times the risk of developing ovarian cancer as parous women. Unmarried women had 3 times the risk of developing ovarian cancer, but marital status was not established as a variable independent of parity. Almost 8% of patients with ovarian carcinoma had had hysterectomies when they were over 40 years of age. This study indicates that parity is an important consideration when determining whether a patient is at high risk for the development of ovarian carcinoma. Other epidemiologic variables are discussed in an effort to facilitate the judicious selection of patients for elective oophorectomy at the time of hysterectomy.", "contents": "The association of parity and marital status with the development of ovarian carcinoma: clinical implications. Three hundred twenty-seven patients with ovarian carcinoma were compared with matched controls to determine the association of parity and marital status with the development of ovarian cancer. Nulliparous women were found to have 2.31 times the risk of developing ovarian cancer as parous women. Unmarried women had 3 times the risk of developing ovarian cancer, but marital status was not established as a variable independent of parity. Almost 8% of patients with ovarian carcinoma had had hysterectomies when they were over 40 years of age. This study indicates that parity is an important consideration when determining whether a patient is at high risk for the development of ovarian carcinoma. Other epidemiologic variables are discussed in an effort to facilitate the judicious selection of patients for elective oophorectomy at the time of hysterectomy."}
{"id": "PMID:460748", "title": "Thermographic screening for breast cancer in a gynecologic practice.", "content": "In the course of their practice 2 gynecologists have conducted a program of screening for breast cancer in 1538 patients ages 35-49 years and on 1102 patients ages 50 or older. The screening protocol of breast examinations, thermography, and selective mammography is described. Twenty-four (9/1000) breast cancers were found, 12 in each age group. Eight of these (3/1000) were clinically occult (6 in the younger age group, 4/1000) and were detected by abnormal thermograms followed by mammography. Of the 12 cancers in the 35-49-year-old age group, 6 were discovered at the first screening, and the other 6 by thermographic changes at intervals of 6-24 months. Thermographic screening is more accurate than breast examination alone, but less accurate than if mammography is included. However, since routine annual use of the latter in younger women still raises concerns about x-ray dosage, thermography is a valuable adjunct to physical examination alone. When it is abnormal, mammography is indicated. The 3 complementary methods can reveal about 9 of 10 preclinical cancers in young women.", "contents": "Thermographic screening for breast cancer in a gynecologic practice. In the course of their practice 2 gynecologists have conducted a program of screening for breast cancer in 1538 patients ages 35-49 years and on 1102 patients ages 50 or older. The screening protocol of breast examinations, thermography, and selective mammography is described. Twenty-four (9/1000) breast cancers were found, 12 in each age group. Eight of these (3/1000) were clinically occult (6 in the younger age group, 4/1000) and were detected by abnormal thermograms followed by mammography. Of the 12 cancers in the 35-49-year-old age group, 6 were discovered at the first screening, and the other 6 by thermographic changes at intervals of 6-24 months. Thermographic screening is more accurate than breast examination alone, but less accurate than if mammography is included. However, since routine annual use of the latter in younger women still raises concerns about x-ray dosage, thermography is a valuable adjunct to physical examination alone. When it is abnormal, mammography is indicated. The 3 complementary methods can reveal about 9 of 10 preclinical cancers in young women."}
{"id": "PMID:460749", "title": "Topical 5-fluorouracil in the treatment of intraepithelial neoplasia of the vagina.", "content": "Twelve patients with vaginal intraepithelial neoplasia received topical therapy with 5% 5-fluorouracil cream. Six patients had carcinoma in situ, 5 had moderate dysplasia, and 1 had mild dysplasia. Five patients responded to 1 treatment course. Six patients required 2, and 1 patient required 3 courses of therapy to respond. Three patients developed recurrent vaginal intraepithelial neoplasia 11-16 months after therapy and were retreated with topical 5-fluorouracil. Vaginal irritation occurred in all patients but was limited to the duration of the treatment course.", "contents": "Topical 5-fluorouracil in the treatment of intraepithelial neoplasia of the vagina. Twelve patients with vaginal intraepithelial neoplasia received topical therapy with 5% 5-fluorouracil cream. Six patients had carcinoma in situ, 5 had moderate dysplasia, and 1 had mild dysplasia. Five patients responded to 1 treatment course. Six patients required 2, and 1 patient required 3 courses of therapy to respond. Three patients developed recurrent vaginal intraepithelial neoplasia 11-16 months after therapy and were retreated with topical 5-fluorouracil. Vaginal irritation occurred in all patients but was limited to the duration of the treatment course."}
{"id": "PMID:460750", "title": "Ovarian remnant syndrome.", "content": "The ovarian remnant syndrome is one of the least recognized and least discussed complications of oophorectomy. The most common presentation is pelvic pain with a pelvic mass. To prevent the condition, the ureters should be mobilized and laterally displaced during oophorectomy, and the pelvic side wall and cul-de-sac peritoneum should be excised when the ovary is adherent to these areas as a result of endometriosis or inflammation.", "contents": "Ovarian remnant syndrome. The ovarian remnant syndrome is one of the least recognized and least discussed complications of oophorectomy. The most common presentation is pelvic pain with a pelvic mass. To prevent the condition, the ureters should be mobilized and laterally displaced during oophorectomy, and the pelvic side wall and cul-de-sac peritoneum should be excised when the ovary is adherent to these areas as a result of endometriosis or inflammation."}
{"id": "PMID:460751", "title": "Minilaparotomy for female sterilization.", "content": "Minilaparotomy, as described in this report, is surgical entry into the lower abdominal cavity through a small transverse suprapubic incision, for the purpose of tubal ligation. The 200 operations reported were performed under local anesthesia in a free-standing office surgical unit. For most women requesting sterilization, minilaparotomy is a highly satisfactory approach. For patients with fixed uterine retroversion, enlarged uteri, or suspected adnexal disease, open laparoscopy under local anesthesia is preferable. The standard Pomeroy technique was used in all cases. Analysis of a follow-up minilaparotomy questionnaire indicates a high degree of patient satisfaction. No subsequent pregnancies have been reported by these patients, but since no patient in this series has been followed for more than 2 years, no conclusion as to long-term failure rate may be made.", "contents": "Minilaparotomy for female sterilization. Minilaparotomy, as described in this report, is surgical entry into the lower abdominal cavity through a small transverse suprapubic incision, for the purpose of tubal ligation. The 200 operations reported were performed under local anesthesia in a free-standing office surgical unit. For most women requesting sterilization, minilaparotomy is a highly satisfactory approach. For patients with fixed uterine retroversion, enlarged uteri, or suspected adnexal disease, open laparoscopy under local anesthesia is preferable. The standard Pomeroy technique was used in all cases. Analysis of a follow-up minilaparotomy questionnaire indicates a high degree of patient satisfaction. No subsequent pregnancies have been reported by these patients, but since no patient in this series has been followed for more than 2 years, no conclusion as to long-term failure rate may be made."}
{"id": "PMID:460752", "title": "Luteal deficiency among women with normal menstrual cycles, requesting reversal of tubal sterilization.", "content": "Forty women with normal menstrual cycles who had been sterilized by tubal ligation or electrocoagulation requested tubal reconstruction. As part of their preoperative evaluation, progesterone measurements were obtained in the midluteal phase (5-10 days before the next menstrual period). This group of women had a significantly lower (P less than 0.005) mean midluteal progesterone level (9.4 +/- 4.7 ng/ml) than a control group of 24 women with infertile male partners attending the same clinic (17.4 +/- 7.1 ng/ml). In 25 (62%) of the sterilized women, progesterone levels were less than 10 ng/ml, whereas in the control group such low values were found in only 4 (17%) of the women. Thus, reduced midluteal serum progesterone concentration appears more common among women with prior tubal ligation or electrocoagulation than among a control population of apparently normal women.", "contents": "Luteal deficiency among women with normal menstrual cycles, requesting reversal of tubal sterilization. Forty women with normal menstrual cycles who had been sterilized by tubal ligation or electrocoagulation requested tubal reconstruction. As part of their preoperative evaluation, progesterone measurements were obtained in the midluteal phase (5-10 days before the next menstrual period). This group of women had a significantly lower (P less than 0.005) mean midluteal progesterone level (9.4 +/- 4.7 ng/ml) than a control group of 24 women with infertile male partners attending the same clinic (17.4 +/- 7.1 ng/ml). In 25 (62%) of the sterilized women, progesterone levels were less than 10 ng/ml, whereas in the control group such low values were found in only 4 (17%) of the women. Thus, reduced midluteal serum progesterone concentration appears more common among women with prior tubal ligation or electrocoagulation than among a control population of apparently normal women."}
{"id": "PMID:460753", "title": "Cefoxitin: single-agent treatment of mixed aerobic-anaerobic pelvic infections.", "content": "Cefoxitin (mefoxin), a new semisynthetic cephamycin antibiotic, resistant to degradation by beta-lactamase enzymes produced by bacteria. In vitro, cefoxitin is active against virtually all clinically important gram-negative facultative bacteria other than Pseudomonas and Enterobacter spp., gram-positive aerobic bacteria other than the enterococcus, and clinically important anaerobic organisms, including Bacteroides fragilis. This broad antibacterial spectrum suggested that cefoxitin might be an effective single antibiotic agent for the treatment of mixed aerobic-anaerobic infections in obstetric and gynecologic patients. In this investigation, the efficacy and safety of cefoxitin was evaluated in 109 patients--68 with salpingitis, 25 with endomyometritis, 9 with pelvic cellulitis, and 7 with pelvic abscesses. An average of 2.5 bacteria were isolated from each patient. Aerobic bacteria alone was isolated in 38% of patients, anaerobic bacteria alone in 25%, and a combination of aerobic and anaerobic bacteria was isolated in 37% of patients. Overall, 100 of 109 (92%) infections responded to treatment with cefoxitin alone. The major cause of treatment failure was the presence of abscesses requiring surgical drainage. In addition to being an effective single agent for the management of pelvic infections, cefoxitin proved to be safe and well tolerated by patients.", "contents": "Cefoxitin: single-agent treatment of mixed aerobic-anaerobic pelvic infections. Cefoxitin (mefoxin), a new semisynthetic cephamycin antibiotic, resistant to degradation by beta-lactamase enzymes produced by bacteria. In vitro, cefoxitin is active against virtually all clinically important gram-negative facultative bacteria other than Pseudomonas and Enterobacter spp., gram-positive aerobic bacteria other than the enterococcus, and clinically important anaerobic organisms, including Bacteroides fragilis. This broad antibacterial spectrum suggested that cefoxitin might be an effective single antibiotic agent for the treatment of mixed aerobic-anaerobic infections in obstetric and gynecologic patients. In this investigation, the efficacy and safety of cefoxitin was evaluated in 109 patients--68 with salpingitis, 25 with endomyometritis, 9 with pelvic cellulitis, and 7 with pelvic abscesses. An average of 2.5 bacteria were isolated from each patient. Aerobic bacteria alone was isolated in 38% of patients, anaerobic bacteria alone in 25%, and a combination of aerobic and anaerobic bacteria was isolated in 37% of patients. Overall, 100 of 109 (92%) infections responded to treatment with cefoxitin alone. The major cause of treatment failure was the presence of abscesses requiring surgical drainage. In addition to being an effective single agent for the management of pelvic infections, cefoxitin proved to be safe and well tolerated by patients."}
{"id": "PMID:460754", "title": "Semiquantitative use of Biocept-G radioreceptorassay.", "content": "Serum samples (93 total) from 62 different patients were analyzed by quantitative radioimmunoassay (RIA) and by the Biocept-G radioreceptorassay (RRA) for detection of human chorionic gonadotropin (hCG). The ratio of sample counts to reference control counts (S/R) was determined for each RRA. For the 44 serum samples having S/R ratios between 0.295 and 1.20 (corresponding to hCG levels of 4600 and 89 mIU/ml, respectively), there was good correlation between the S/R ratio by RRA and the serum level of hCG by RIA (correlation coefficient = -0.92). When serum samples were analyzed in as many as 4 different Biocept-G kits, the same S/R ratios were obtained. The S/R ratio reliably predicted the trend in serum hCG titer in 7 patients during treatment for gestational trophoblastic disease. The usefulness of the S/R ratio in obtaining semiquantitative results with the Biocept-G RRA is discussed.", "contents": "Semiquantitative use of Biocept-G radioreceptorassay. Serum samples (93 total) from 62 different patients were analyzed by quantitative radioimmunoassay (RIA) and by the Biocept-G radioreceptorassay (RRA) for detection of human chorionic gonadotropin (hCG). The ratio of sample counts to reference control counts (S/R) was determined for each RRA. For the 44 serum samples having S/R ratios between 0.295 and 1.20 (corresponding to hCG levels of 4600 and 89 mIU/ml, respectively), there was good correlation between the S/R ratio by RRA and the serum level of hCG by RIA (correlation coefficient = -0.92). When serum samples were analyzed in as many as 4 different Biocept-G kits, the same S/R ratios were obtained. The S/R ratio reliably predicted the trend in serum hCG titer in 7 patients during treatment for gestational trophoblastic disease. The usefulness of the S/R ratio in obtaining semiquantitative results with the Biocept-G RRA is discussed."}
{"id": "PMID:460755", "title": "Human placental lactogen: a predictor of perinatal outcome?", "content": "Serial human placental lactogen (hPL) determinations were performed on 806 women with normal and abnormal pregnancies late in the pregnancy. These results were not reported to the clinicians involved. For the study population as a whole, low hPL levels did not effectively predict those adverse perinatal outcome variables evaluated. Further analysis revealed that this was true both for the normal and abnormal pregnancy groups. Our data do not support the routine use of antepartum hPL screening, as advocated by others, as a means of improving perinatal outcome. In certain at-risk patients, there was an association between low hPL values and the presence of 1 or more of the adverse outcome variables. However, these patients had been recognized clinically as having fetuses in jeopardy.", "contents": "Human placental lactogen: a predictor of perinatal outcome? Serial human placental lactogen (hPL) determinations were performed on 806 women with normal and abnormal pregnancies late in the pregnancy. These results were not reported to the clinicians involved. For the study population as a whole, low hPL levels did not effectively predict those adverse perinatal outcome variables evaluated. Further analysis revealed that this was true both for the normal and abnormal pregnancy groups. Our data do not support the routine use of antepartum hPL screening, as advocated by others, as a means of improving perinatal outcome. In certain at-risk patients, there was an association between low hPL values and the presence of 1 or more of the adverse outcome variables. However, these patients had been recognized clinically as having fetuses in jeopardy."}
{"id": "PMID:460756", "title": "Patterns of intrauterine growth retardation.", "content": "A prospective study of 70 singleton pregnancies at high risk for intrauterine growth retardation (IUGR) was undertaken to determine 1) the differences in intrauterine growth patterns; 2) the diagnostic accuracy of obstetric techniques; and 3) the frequencies of perinatal complications. Thirty infants displayed signs of IUGR. Although only 14 infants had low birth weights, these 14, as well as the remaining 16 infants under study, displayed many other features of growth abnormalities, including a low ponderal index, short stature, and small head circumference. These data demonstrate various patterns of IUGR. Although the perinatal complications occurred primarily in the low-birth-weight group, the major growth abnormalities observed in the non-low-birth-weight group demonstrate the need for additional short- and long-term follow-up studies in both groups.", "contents": "Patterns of intrauterine growth retardation. A prospective study of 70 singleton pregnancies at high risk for intrauterine growth retardation (IUGR) was undertaken to determine 1) the differences in intrauterine growth patterns; 2) the diagnostic accuracy of obstetric techniques; and 3) the frequencies of perinatal complications. Thirty infants displayed signs of IUGR. Although only 14 infants had low birth weights, these 14, as well as the remaining 16 infants under study, displayed many other features of growth abnormalities, including a low ponderal index, short stature, and small head circumference. These data demonstrate various patterns of IUGR. Although the perinatal complications occurred primarily in the low-birth-weight group, the major growth abnormalities observed in the non-low-birth-weight group demonstrate the need for additional short- and long-term follow-up studies in both groups."}
{"id": "PMID:460757", "title": "Predictability of gestational hypertension.", "content": "Eighty-five women at 28-36 weeks' gestation were studied prospectively in a clinical research center to assess a \"roll over test\" (RT) based on the original study by Gant et al. Patients were admitted to the hospital, placed on bed rest, and given a balanced diet. The test started 12 hours after admission. Blood pressure was measured every 10 minutes for an hour with the patient in the left lateral position, and then in the supine position for a second hour. The mean diastolic blood pressure was calculated in each position and the patients were sorted into 6 groups based on the magnitude of change in blood pressure. Seventeen patients developed gestational hypertension (GH). Only 3 of 20 (15%) patients with a positive RT developed GH, while 14 of 65 (22%) patients with a negative RT developed GH. Five of 12 patients who had an actual decrease in mean diastolic blood pressure also developed GH.", "contents": "Predictability of gestational hypertension. Eighty-five women at 28-36 weeks' gestation were studied prospectively in a clinical research center to assess a \"roll over test\" (RT) based on the original study by Gant et al. Patients were admitted to the hospital, placed on bed rest, and given a balanced diet. The test started 12 hours after admission. Blood pressure was measured every 10 minutes for an hour with the patient in the left lateral position, and then in the supine position for a second hour. The mean diastolic blood pressure was calculated in each position and the patients were sorted into 6 groups based on the magnitude of change in blood pressure. Seventeen patients developed gestational hypertension (GH). Only 3 of 20 (15%) patients with a positive RT developed GH, while 14 of 65 (22%) patients with a negative RT developed GH. Five of 12 patients who had an actual decrease in mean diastolic blood pressure also developed GH."}
{"id": "PMID:460758", "title": "The use of amniocentesis in patients with premature rupture of membranes.", "content": "A prospective study was conducted to evaluate the usefulness of amniocentesis in patients with premature rupture of membranes (PROM) for the prediction of occult or impending intrauterine infection and the assessment of fetal maturity. Fifty-nine patients with PROM between 28 and 35 weeks' gestation, without apparent infection, were evaluated sonographically for possible amniocentesis. Thirty patients had successful amniocenteses for a success rate of 51%. Fifteen patients had a mature lecithin/sphingomyelin (L/S) ratio and were delivered immediately without respiratory distress syndrome (RDS) or other neonatal complications. All amniotic fluids were evaluated by Gram stains and cultures. Nine of the 30 patients had positive cultures, with a high incidence of subsequent development of amnionitis and other infectious morbidity. In the 21 patients with negative cultures, there were 19 without any infectious morbidity. Amniocentesis appears in this study to be a useful method for selecting the patients who have mature fetuses and/or who are more likely to develop amnionitis.", "contents": "The use of amniocentesis in patients with premature rupture of membranes. A prospective study was conducted to evaluate the usefulness of amniocentesis in patients with premature rupture of membranes (PROM) for the prediction of occult or impending intrauterine infection and the assessment of fetal maturity. Fifty-nine patients with PROM between 28 and 35 weeks' gestation, without apparent infection, were evaluated sonographically for possible amniocentesis. Thirty patients had successful amniocenteses for a success rate of 51%. Fifteen patients had a mature lecithin/sphingomyelin (L/S) ratio and were delivered immediately without respiratory distress syndrome (RDS) or other neonatal complications. All amniotic fluids were evaluated by Gram stains and cultures. Nine of the 30 patients had positive cultures, with a high incidence of subsequent development of amnionitis and other infectious morbidity. In the 21 patients with negative cultures, there were 19 without any infectious morbidity. Amniocentesis appears in this study to be a useful method for selecting the patients who have mature fetuses and/or who are more likely to develop amnionitis."}
{"id": "PMID:460759", "title": "Early diagnosis of placenta previa.", "content": "Ultrasound was used as a diagnostic tool in 859 patients for the localization of the placenta in the second trimester prior to genetic amniocentesis. A greater incidence of total placenta previa was found in the study group (5.6%) than is reported for the general population (0.25-0.5%). Maternal age greater than 35 years was the indication or co-factor in 59% of the patients studied, which might explain this difference. It was possible to predict total placenta previa at term early in the second trimester. It was demonstrated that if a placenta was located centrally over the cervical os and did not change position with variations in the degree of bladder-filling or changes in uterine position, the risk of having a total placenta previa at term increased from 1/143 to 1/8 pregnancies.", "contents": "Early diagnosis of placenta previa. Ultrasound was used as a diagnostic tool in 859 patients for the localization of the placenta in the second trimester prior to genetic amniocentesis. A greater incidence of total placenta previa was found in the study group (5.6%) than is reported for the general population (0.25-0.5%). Maternal age greater than 35 years was the indication or co-factor in 59% of the patients studied, which might explain this difference. It was possible to predict total placenta previa at term early in the second trimester. It was demonstrated that if a placenta was located centrally over the cervical os and did not change position with variations in the degree of bladder-filling or changes in uterine position, the risk of having a total placenta previa at term increased from 1/143 to 1/8 pregnancies."}
{"id": "PMID:460760", "title": "Positive rubella titers in pregnancy: a question of validity.", "content": "A review of 25 patients with more than 1 rubella titer by hemagglutination-inhibition testing revealed 3 patients with high positive titers that were reported to be negative in subsequent pregnancies. None of the patients had received the rubella vaccine. Because the sensitivity and reliability of hemagglutination-inhibition testing is markedly influenced by multiple variables, it is important for both the clinician and the pathologist to be aware of both the limitations of the testing method and the meaning of an antibody titer on a single isolated specimen.", "contents": "Positive rubella titers in pregnancy: a question of validity. A review of 25 patients with more than 1 rubella titer by hemagglutination-inhibition testing revealed 3 patients with high positive titers that were reported to be negative in subsequent pregnancies. None of the patients had received the rubella vaccine. Because the sensitivity and reliability of hemagglutination-inhibition testing is markedly influenced by multiple variables, it is important for both the clinician and the pathologist to be aware of both the limitations of the testing method and the meaning of an antibody titer on a single isolated specimen."}
{"id": "PMID:460761", "title": "A Simplified antepartum risk-scoring system.", "content": "The effectiveness of a simple antepartum risk-scoring system was evaluated in 2085 consecutive deliveries. Neonatal morbidity was observed in 42.1% of infants of mothers classified as high risk (score greater than or equal to 7) compared to 12.5% of infants of mothers classified as low risk (score less than 7). No neonatal deaths were observed in the low-risk group, compared with 34 in the high-risk group (P less than 0.001). Of all perinatal deaths, 88.6% occurred in the high-risk group. The perinatal mortality rates for low- and high-risk pregnancies were 7.2 and 63.3, respectively, per 1000 live births.", "contents": "A Simplified antepartum risk-scoring system. The effectiveness of a simple antepartum risk-scoring system was evaluated in 2085 consecutive deliveries. Neonatal morbidity was observed in 42.1% of infants of mothers classified as high risk (score greater than or equal to 7) compared to 12.5% of infants of mothers classified as low risk (score less than 7). No neonatal deaths were observed in the low-risk group, compared with 34 in the high-risk group (P less than 0.001). Of all perinatal deaths, 88.6% occurred in the high-risk group. The perinatal mortality rates for low- and high-risk pregnancies were 7.2 and 63.3, respectively, per 1000 live births."}
{"id": "PMID:460762", "title": "Ileus of the colon with cecal dilatation and perforation.", "content": "Segmental dilatation of the colon without obstruction is an unusual but recognizable entity, distinct from mechanical obstruction and paralytic ileus. Cases of ileus of the colon with cecal dilatation following delivery and gynecologic surgery are collected from the literature, and 3 recent cases are presented: 1 following cesarean section and 2 following abdominal hysterectomy. The etiology of this condition is still obscure and the clinical features are deceptive. The occurrence of this entity in obstetric and gynecologic patients is by no means a rarity and warrants a familiarity with and early recognition of this entity on the part of the physician in order to avoid serious sequelae.", "contents": "Ileus of the colon with cecal dilatation and perforation. Segmental dilatation of the colon without obstruction is an unusual but recognizable entity, distinct from mechanical obstruction and paralytic ileus. Cases of ileus of the colon with cecal dilatation following delivery and gynecologic surgery are collected from the literature, and 3 recent cases are presented: 1 following cesarean section and 2 following abdominal hysterectomy. The etiology of this condition is still obscure and the clinical features are deceptive. The occurrence of this entity in obstetric and gynecologic patients is by no means a rarity and warrants a familiarity with and early recognition of this entity on the part of the physician in order to avoid serious sequelae."}
{"id": "PMID:460763", "title": "Acute pancreatitis and hyperparathyroidism in pregnancy.", "content": "A case of acute pancreatitis associated with hyperparathyroidism in the third trimester of pregnancy is summarized, and the clinical findings are discussed. Emphasis is placed on recognition of pancreatitis and its etiology in the third trimester of pregnancy. The surgical approach to the parathyroid pathology is analyzed and its advantages espoused.", "contents": "Acute pancreatitis and hyperparathyroidism in pregnancy. A case of acute pancreatitis associated with hyperparathyroidism in the third trimester of pregnancy is summarized, and the clinical findings are discussed. Emphasis is placed on recognition of pancreatitis and its etiology in the third trimester of pregnancy. The surgical approach to the parathyroid pathology is analyzed and its advantages espoused."}
{"id": "PMID:460764", "title": "Evaluation of fetal monitoring by telemetry.", "content": "A radiotelemetry system for the direct monitoring of fetal heart rate and intrauterine pressure during labor has been evaluated at 5 different institutions. A conventional fetal scalp electrode and a special intrauterine pressure sensor are connected to a radio transmitter placed on the patient's thigh. The receiver can be located up to 50 feet away from the transmitter and is either a self-contained monitor or a unit that converts a conventional fetal monitor to a telemetric one. The telemetry recordings are of similar quality to those obtained from conventional monitors. Telemetry allows for greater patient comfort and mobility as well as greater convenience to the clinical staff. Continuous data can be obtained from patients while they are ambulatory or sitting in a chair as well as while they are in bed.", "contents": "Evaluation of fetal monitoring by telemetry. A radiotelemetry system for the direct monitoring of fetal heart rate and intrauterine pressure during labor has been evaluated at 5 different institutions. A conventional fetal scalp electrode and a special intrauterine pressure sensor are connected to a radio transmitter placed on the patient's thigh. The receiver can be located up to 50 feet away from the transmitter and is either a self-contained monitor or a unit that converts a conventional fetal monitor to a telemetric one. The telemetry recordings are of similar quality to those obtained from conventional monitors. Telemetry allows for greater patient comfort and mobility as well as greater convenience to the clinical staff. Continuous data can be obtained from patients while they are ambulatory or sitting in a chair as well as while they are in bed."}
{"id": "PMID:460765", "title": "Vaginal vault prolapse with eversion.", "content": "The successful treatment of vaginal vault prolapse with eversion continues to be an enigma to surgeons. The many surgical procedures that have been proposed testify to the fact that no single reliable procedure has been developed. The vaginal operation of posterior pelvic shelf colpopexy described in this report represents an evolution of ideas drawn from other surgeons coupled with the author's personal experience. The concept of creating a high, posterior pelvic shelf for vaginal vault fixation is developed, and the surgical technique is discussed in detail.", "contents": "Vaginal vault prolapse with eversion. The successful treatment of vaginal vault prolapse with eversion continues to be an enigma to surgeons. The many surgical procedures that have been proposed testify to the fact that no single reliable procedure has been developed. The vaginal operation of posterior pelvic shelf colpopexy described in this report represents an evolution of ideas drawn from other surgeons coupled with the author's personal experience. The concept of creating a high, posterior pelvic shelf for vaginal vault fixation is developed, and the surgical technique is discussed in detail."}
{"id": "PMID:460766", "title": "The Kevorkian curette. An appraisal of its effectiveness in endometrial evaluation.", "content": "Office endometrial biopsy with the Kevorkian curette was performed in 400 patients presenting with abnormal uterine bleeding or other endometrial cancer-risk indicators. The use of this instrument has proved to be a safe, simple, inexpensive, and highly reliable outpatient procedure with excellent patient acceptance. It has provided tissue for adequate diagnosis in 91.8% of the cases, and the diagnostic accuracy when controlled by D&C and/or total abdominal hysterectomy (TAH) was 96.2%. As a result, 73.5% of the women required no further surgical procedures for either diagnostic or therapeutic purposes. In the postmenopausal age group, adenocarcinoma was diagnosed in 7 of 177 (3.9%) women. This procedure is highly recommended for early office diagnosis of uterine pathology and, in particular, endometrial adenocarcinoma and its presumed precursor lesion, adenomatous hyperplasia.", "contents": "The Kevorkian curette. An appraisal of its effectiveness in endometrial evaluation. Office endometrial biopsy with the Kevorkian curette was performed in 400 patients presenting with abnormal uterine bleeding or other endometrial cancer-risk indicators. The use of this instrument has proved to be a safe, simple, inexpensive, and highly reliable outpatient procedure with excellent patient acceptance. It has provided tissue for adequate diagnosis in 91.8% of the cases, and the diagnostic accuracy when controlled by D&C and/or total abdominal hysterectomy (TAH) was 96.2%. As a result, 73.5% of the women required no further surgical procedures for either diagnostic or therapeutic purposes. In the postmenopausal age group, adenocarcinoma was diagnosed in 7 of 177 (3.9%) women. This procedure is highly recommended for early office diagnosis of uterine pathology and, in particular, endometrial adenocarcinoma and its presumed precursor lesion, adenomatous hyperplasia."}
{"id": "PMID:460788", "title": "Variations in the level of haematogenous antitumour immunity during progressive tumour growth and spontaneous blood-borne metastatic spread.", "content": "Inbred DBA2 mice bearing the syngeneic 1699 mammary tumour in the hind limb were challenged intravenously with 125IUdR-labelled single 1699 cell suspensions, and the amount of radioactivity in the lungs compared 20--24 h later with that in the lungs of normal mice or in those of mice bearing the antigenically unrelated syngeneic SaD2 tumour. An immunologically specific decrease in radioactivity was evident at variable periods of time after tumour induction, depending on the number of cells used to induce the leg tumours, but fell below that in normal mice as the leg tumours progressed beyond a weight of approximately 1 g. As assessed by microscopic scanning of serial histological sections of the same lungs the incidence of spontaneous metastases rose to between 80 and 100% immediately the amount of cell loss from the lungs of the tumour-bearing mice reached that of the normal controls. This extremely rapid series of events does not allow a definitive conclusion as to whether immunity failed and led to metastatic spread or vice versa, but does underline the strong association of immunity with the blood-borne dissemination of tumour cells in this tumour system. Following excision of tumours, in no instance was immunity detected 14 days later, and in a single experiment did not reach detectable limits until 25 days after excision, at a time when the lung metastases were observed mostly to have regressed spontaneously.", "contents": "Variations in the level of haematogenous antitumour immunity during progressive tumour growth and spontaneous blood-borne metastatic spread. Inbred DBA2 mice bearing the syngeneic 1699 mammary tumour in the hind limb were challenged intravenously with 125IUdR-labelled single 1699 cell suspensions, and the amount of radioactivity in the lungs compared 20--24 h later with that in the lungs of normal mice or in those of mice bearing the antigenically unrelated syngeneic SaD2 tumour. An immunologically specific decrease in radioactivity was evident at variable periods of time after tumour induction, depending on the number of cells used to induce the leg tumours, but fell below that in normal mice as the leg tumours progressed beyond a weight of approximately 1 g. As assessed by microscopic scanning of serial histological sections of the same lungs the incidence of spontaneous metastases rose to between 80 and 100% immediately the amount of cell loss from the lungs of the tumour-bearing mice reached that of the normal controls. This extremely rapid series of events does not allow a definitive conclusion as to whether immunity failed and led to metastatic spread or vice versa, but does underline the strong association of immunity with the blood-borne dissemination of tumour cells in this tumour system. Following excision of tumours, in no instance was immunity detected 14 days later, and in a single experiment did not reach detectable limits until 25 days after excision, at a time when the lung metastases were observed mostly to have regressed spontaneously."}
{"id": "PMID:460789", "title": "Comparative study on drug sensitivity of the TA3/Ha and TA3/St tumor sublines.", "content": "TA3/Ha and TA3/St sublines differ from each other in some characteristics, like malignancy, survival time and drug sensitivity. TA3/St exhibited a peculiar response to the Vinca derivatives examined.", "contents": "Comparative study on drug sensitivity of the TA3/Ha and TA3/St tumor sublines. TA3/Ha and TA3/St sublines differ from each other in some characteristics, like malignancy, survival time and drug sensitivity. TA3/St exhibited a peculiar response to the Vinca derivatives examined."}
{"id": "PMID:460790", "title": "Bone scintigrams: their clinical usefulness in patients with breast carcinoma.", "content": "A comparison of bone scintigrams and roentgenographic skeletal surveys, obtained on 170 patients with breast carcinoma, was made to evaluate the diagnostic efficacy of these techniques in detecting metastatic bone lesions. The bone scans were abnormal in 81 patients, while the roentgenograms were abnormal in only 51. In 34 of the 81 (42%) patients with abnormal bone scans, there was no radiographic evidence of a benign lesion to account for the increased ratiotracer uptake; and the abnormalities noted on the bone scans were proven to be bony metastases on follow-up examinations. In the remaining 47 patients with positive bone scintigrams, both the scans and the roentgenograms were abnormal; however, in 23 patients the bone scan demonstrated significantly more lesions than what the roentgenograms had revealed. This study confirms that bone scan is superior to roentgen surveys in detecting skeletal metastases in patients with breast carcinoma. It was noted that the metastatic lesions can be visualized on the scans earlier than they are apparent on the X-rays by a mean interval of 4 months.", "contents": "Bone scintigrams: their clinical usefulness in patients with breast carcinoma. A comparison of bone scintigrams and roentgenographic skeletal surveys, obtained on 170 patients with breast carcinoma, was made to evaluate the diagnostic efficacy of these techniques in detecting metastatic bone lesions. The bone scans were abnormal in 81 patients, while the roentgenograms were abnormal in only 51. In 34 of the 81 (42%) patients with abnormal bone scans, there was no radiographic evidence of a benign lesion to account for the increased ratiotracer uptake; and the abnormalities noted on the bone scans were proven to be bony metastases on follow-up examinations. In the remaining 47 patients with positive bone scintigrams, both the scans and the roentgenograms were abnormal; however, in 23 patients the bone scan demonstrated significantly more lesions than what the roentgenograms had revealed. This study confirms that bone scan is superior to roentgen surveys in detecting skeletal metastases in patients with breast carcinoma. It was noted that the metastatic lesions can be visualized on the scans earlier than they are apparent on the X-rays by a mean interval of 4 months."}
{"id": "PMID:460794", "title": "[Cell cycles in the early embryogenesis of the peled].", "content": "The dynamics of cell cycles in early embryogenesis of the peled has been studied using various methofs: determination of mitotic and mitotic phase index, of cell doubling time and of coefficient of asynchrony. The desynchronization of divisions begins at the V division after the longitudinal furrow has passed and is completed after the beginning of the cell cycle lengthening. The animal-vegetative gradient in the distribution of mitotic phases is observed at the early stages of desynchronization. The lengthening of cell cycle begins in interphase of the XI-XII cleavage and markedly accelerates from the interphase of the XII-XIII cleavage on. The methods of analysis of the cell cycles in early embryogenesis and possible factors influencing the dynamics of desynchronization are discussed.", "contents": "[Cell cycles in the early embryogenesis of the peled]. The dynamics of cell cycles in early embryogenesis of the peled has been studied using various methofs: determination of mitotic and mitotic phase index, of cell doubling time and of coefficient of asynchrony. The desynchronization of divisions begins at the V division after the longitudinal furrow has passed and is completed after the beginning of the cell cycle lengthening. The animal-vegetative gradient in the distribution of mitotic phases is observed at the early stages of desynchronization. The lengthening of cell cycle begins in interphase of the XI-XII cleavage and markedly accelerates from the interphase of the XII-XIII cleavage on. The methods of analysis of the cell cycles in early embryogenesis and possible factors influencing the dynamics of desynchronization are discussed."}
{"id": "PMID:460795", "title": "[Morphology of the microtubule system in mouse kidney epithelial cells].", "content": "The cultured mouse kidney cells forming epithelial sheets were studied using an indirect immunofluorescence microscopy with antibodies against tubulin. These cells, as well as fibroblasts, were found to contain a well developed microtubular system sensitive to colcemid. The assembly of microtubules after washing out of colcemid began from one or two perinuclear centers, associated with the cilium-like structure. There were certain differences between the microtubular systems in epithelial cells and fibroblasts: 1) Microtubules in the fibroblasts penetrated the whole cytoplasm including the peripheral lamella whereas in the epithelial cells the lamellar cytoplasm was often free from microtubules. 2) The orientation of microtubules in the epithelial cells, unlike in the fibroblasts, was not correlated with the stable or active state of the cell margin. A possible role of microtubular system in the epithelial cells and fibroblasts is compared and discussed.", "contents": "[Morphology of the microtubule system in mouse kidney epithelial cells]. The cultured mouse kidney cells forming epithelial sheets were studied using an indirect immunofluorescence microscopy with antibodies against tubulin. These cells, as well as fibroblasts, were found to contain a well developed microtubular system sensitive to colcemid. The assembly of microtubules after washing out of colcemid began from one or two perinuclear centers, associated with the cilium-like structure. There were certain differences between the microtubular systems in epithelial cells and fibroblasts: 1) Microtubules in the fibroblasts penetrated the whole cytoplasm including the peripheral lamella whereas in the epithelial cells the lamellar cytoplasm was often free from microtubules. 2) The orientation of microtubules in the epithelial cells, unlike in the fibroblasts, was not correlated with the stable or active state of the cell margin. A possible role of microtubular system in the epithelial cells and fibroblasts is compared and discussed."}
{"id": "PMID:460796", "title": "[Nerve fiber interrelationships with the ependyma of the 3d ventricle in rats in the perinatal period].", "content": "The localization and ultrastructure of nerve fibers in the wall and lumen of ventral region of III ventricle was studied from the 16th day of prenatal period till the 9th day of postnatal period in the Wistar rats. The nerve fibers were first found in the subependyme zone; between the ependyme cells and in the lumen of ventricle on the 18th day of development. The nerve fibers occur constantly in the lumen of ventricle beginning from the 3rd day of postnatal period. The dilatations of nerve fibers contain granules of 50 to 90 nm in diameter with the electron-dense center (carriers of monoamines and/or releasing hormonnes) granules with moderately osmiophilic contents and light vesicles of the same size. It is suggested that these structures reflect different stages of the release of neurohormones in the cerebrospinal fluid. The synapse-like contacts of nerve fibers with the ependyme cells are observed beginning from the 3rd day of postnatal period. Their role in the regulation of absorption of substances by the ependyme cells functioning is discussed.", "contents": "[Nerve fiber interrelationships with the ependyma of the 3d ventricle in rats in the perinatal period]. The localization and ultrastructure of nerve fibers in the wall and lumen of ventral region of III ventricle was studied from the 16th day of prenatal period till the 9th day of postnatal period in the Wistar rats. The nerve fibers were first found in the subependyme zone; between the ependyme cells and in the lumen of ventricle on the 18th day of development. The nerve fibers occur constantly in the lumen of ventricle beginning from the 3rd day of postnatal period. The dilatations of nerve fibers contain granules of 50 to 90 nm in diameter with the electron-dense center (carriers of monoamines and/or releasing hormonnes) granules with moderately osmiophilic contents and light vesicles of the same size. It is suggested that these structures reflect different stages of the release of neurohormones in the cerebrospinal fluid. The synapse-like contacts of nerve fibers with the ependyme cells are observed beginning from the 3rd day of postnatal period. Their role in the regulation of absorption of substances by the ependyme cells functioning is discussed."}
{"id": "PMID:460797", "title": "[Oxidative phosphorylation in the intact and regenerating liver from a chronobiological viewpoint].", "content": "The dynamics of oxidative phosphorylation of the regenerating liver mitochondria on the endogenous and exogenous (glutamate, succinate, ascorbate) substrates in comparison with the normal diurnal and seasonal changes of this process was studied by the polarographical method. With the increase of its activity reflecting the deviation from the state of physiological rest (intact liver in spring, autumn, winter; regenerating liver), the range and synphasing of the oxidative phosphorylation indices were decreased, their bimodal distributions were transformed into the monomodal ones down to the full loss of diurnal periodicity. The degree of the process desynchronization depends, thus, directly on the degree of its activity.", "contents": "[Oxidative phosphorylation in the intact and regenerating liver from a chronobiological viewpoint]. The dynamics of oxidative phosphorylation of the regenerating liver mitochondria on the endogenous and exogenous (glutamate, succinate, ascorbate) substrates in comparison with the normal diurnal and seasonal changes of this process was studied by the polarographical method. With the increase of its activity reflecting the deviation from the state of physiological rest (intact liver in spring, autumn, winter; regenerating liver), the range and synphasing of the oxidative phosphorylation indices were decreased, their bimodal distributions were transformed into the monomodal ones down to the full loss of diurnal periodicity. The degree of the process desynchronization depends, thus, directly on the degree of its activity."}
{"id": "PMID:460798", "title": "[Cytochemical study of the DNA and histone in the tissues of the lily anther in the course of microsporo- and gametogenesis].", "content": "The DNA and histone content in the microsporocytes, microspores, generative and vegetative cells of the pollen grain of Lilium candidum L., as well as in the anther wall tissues, was estimated by double wavelength cytophotometry. The lack of histone, as compared with DNA content, was demonstrated in the microsporocytes at the late premeiotic interphase and early meiotic prophase, as well as in the young microspores and anther wall tissues. The analysis of hydrolysis curves suggests the increase of non-condensed chromatin during endothelial cell differentiation.", "contents": "[Cytochemical study of the DNA and histone in the tissues of the lily anther in the course of microsporo- and gametogenesis]. The DNA and histone content in the microsporocytes, microspores, generative and vegetative cells of the pollen grain of Lilium candidum L., as well as in the anther wall tissues, was estimated by double wavelength cytophotometry. The lack of histone, as compared with DNA content, was demonstrated in the microsporocytes at the late premeiotic interphase and early meiotic prophase, as well as in the young microspores and anther wall tissues. The analysis of hydrolysis curves suggests the increase of non-condensed chromatin during endothelial cell differentiation."}
{"id": "PMID:460799", "title": "[Cortical reaction of the eggs of the mussel, Crenomytilus grayanus].", "content": "The extrusion of the contents of cortical granules was found in the places of contact of spermatozoa with the egg surface upon fertilization of the mussel eggs. Not all cortical granules broke down, a great number of them is preserved in the developing embryo cells. The possible mechanism and importance of this phenomenon are discussed.", "contents": "[Cortical reaction of the eggs of the mussel, Crenomytilus grayanus]. The extrusion of the contents of cortical granules was found in the places of contact of spermatozoa with the egg surface upon fertilization of the mussel eggs. Not all cortical granules broke down, a great number of them is preserved in the developing embryo cells. The possible mechanism and importance of this phenomenon are discussed."}
{"id": "PMID:460801", "title": "[Change in the electrophysiological parameters of human oocytes in the process of maturation outside the body].", "content": "The dynamics of electrophysiological parameters (membrane potential--MP and resistance--R) of the human oocytes was studied during their in vitro maturation. The relationship was established between the changes of electrophysiological parameters and meiotic phases. The average value of MP of diplotene oocytes was 22.0 +/- 0.3 mV and that of R 2.0 +/- 0.5 mO. The membrane depolarization was observed upon the meiotic reinitiation. The MP of diakinesis--metaphase I oocytes amounted to 10.0 +/- 0.3 mV and R to 10.0 +/- 0.5 mO. At metaphase II the temporary membrane repolarisation was noted in some cases which, in some oocytes, was replaced by the increasing hyperpolarisation on the 2--3rd day of cultivation. The input membrane resistance increased on the 2--3rd day of cultivation.", "contents": "[Change in the electrophysiological parameters of human oocytes in the process of maturation outside the body]. The dynamics of electrophysiological parameters (membrane potential--MP and resistance--R) of the human oocytes was studied during their in vitro maturation. The relationship was established between the changes of electrophysiological parameters and meiotic phases. The average value of MP of diplotene oocytes was 22.0 +/- 0.3 mV and that of R 2.0 +/- 0.5 mO. The membrane depolarization was observed upon the meiotic reinitiation. The MP of diakinesis--metaphase I oocytes amounted to 10.0 +/- 0.3 mV and R to 10.0 +/- 0.5 mO. At metaphase II the temporary membrane repolarisation was noted in some cases which, in some oocytes, was replaced by the increasing hyperpolarisation on the 2--3rd day of cultivation. The input membrane resistance increased on the 2--3rd day of cultivation."}
{"id": "PMID:460802", "title": "[Distribution of acid phosphatase in the chicken blastoderm].", "content": "The distribution of acid phosphatase in the chick blastoderm (stages 2--4 by HH) has been studied using cytochemistry. A marked increase of enzymatic activity all over the blastoderm was shown to coincide with the beginning of primitive streak formation. A part of the cells after their immigration are characterized by the fall of acid phosphatase activity. The percentage of such cells increases in the cranio-caudal direction of the definitive primitive streak. The patterns of yolk utilization upon the separation of individual embryonic rudiments are discussed.", "contents": "[Distribution of acid phosphatase in the chicken blastoderm]. The distribution of acid phosphatase in the chick blastoderm (stages 2--4 by HH) has been studied using cytochemistry. A marked increase of enzymatic activity all over the blastoderm was shown to coincide with the beginning of primitive streak formation. A part of the cells after their immigration are characterized by the fall of acid phosphatase activity. The percentage of such cells increases in the cranio-caudal direction of the definitive primitive streak. The patterns of yolk utilization upon the separation of individual embryonic rudiments are discussed."}
{"id": "PMID:460804", "title": "Techniques in blepharoplasty.", "content": "The technique for upper lid blepharoplasty is presented, which emphasizes the creation of a concave, high supratarsal fold by removal of existing preaponeurotic fat pads, excision of a strip of preseptal orbicularis fibers, and portion of the orbital septum. In additon, the skin edges are fixated to the levator aponeurosis just above the tarsus. With this technique, a pleasing result can be obtained, minimizing the amount of skin that is required to excised and introducing a safety factor in the prevention of postoperative lagophthalmos. A skin technique for lower led blepharoplasty is presented which reduces a tendency for \"dog-ear\" formation with skin closure and minimizes chances of postoperative ectropion. The technique consists of triangulation of the excision of skin from the lower lid to insure an equal-sided wound edge for precise closure and anchoring the lower lid skin flap to the lateral orbital rim. Although the above techniques introduce a safety factor reducing the problem of lagophthalmos in the upper lid and ectropion in the lower lid, they should be considered an \"advanced technique\" by the occasional blepharoplasty surgeon.", "contents": "Techniques in blepharoplasty. The technique for upper lid blepharoplasty is presented, which emphasizes the creation of a concave, high supratarsal fold by removal of existing preaponeurotic fat pads, excision of a strip of preseptal orbicularis fibers, and portion of the orbital septum. In additon, the skin edges are fixated to the levator aponeurosis just above the tarsus. With this technique, a pleasing result can be obtained, minimizing the amount of skin that is required to excised and introducing a safety factor in the prevention of postoperative lagophthalmos. A skin technique for lower led blepharoplasty is presented which reduces a tendency for \"dog-ear\" formation with skin closure and minimizes chances of postoperative ectropion. The technique consists of triangulation of the excision of skin from the lower lid to insure an equal-sided wound edge for precise closure and anchoring the lower lid skin flap to the lateral orbital rim. Although the above techniques introduce a safety factor reducing the problem of lagophthalmos in the upper lid and ectropion in the lower lid, they should be considered an \"advanced technique\" by the occasional blepharoplasty surgeon."}
{"id": "PMID:460806", "title": "Intracapsular cataract extraction at the time of vitrectomy.", "content": "An alternative surgical technique for cataract extraction using an overlapping scleral flap is described for patients in whom removal of the lens is not possible through the pars plana prior to vitrectomy.", "contents": "Intracapsular cataract extraction at the time of vitrectomy. An alternative surgical technique for cataract extraction using an overlapping scleral flap is described for patients in whom removal of the lens is not possible through the pars plana prior to vitrectomy."}
{"id": "PMID:460807", "title": "Malignant melanoma of the choroid presenting as choroidal detachment following cataract surgery.", "content": "The progression of a monocular nuclear cataract was observed for thirty-two months in an eye which was apparently otherwise normal. Three weeks after uncomplicated cataract surgery the patient presented with an apparent detachment of the choroid which enlarged dramatically within a relatively short time and which was further complicated by a retinal detachment. The subsequent development of rubeosis iridis and increased intraocular pressure suggested some other etiology. Ultrasonography revealed a malignant melanoma which was confirmed following enucleation. Transillumination of all suspected postoperative choroidal detachments is recommended with more definitive examination when indicated.", "contents": "Malignant melanoma of the choroid presenting as choroidal detachment following cataract surgery. The progression of a monocular nuclear cataract was observed for thirty-two months in an eye which was apparently otherwise normal. Three weeks after uncomplicated cataract surgery the patient presented with an apparent detachment of the choroid which enlarged dramatically within a relatively short time and which was further complicated by a retinal detachment. The subsequent development of rubeosis iridis and increased intraocular pressure suggested some other etiology. Ultrasonography revealed a malignant melanoma which was confirmed following enucleation. Transillumination of all suspected postoperative choroidal detachments is recommended with more definitive examination when indicated."}
{"id": "PMID:460809", "title": "A fiber-optics cyclodialysis spatula.", "content": "A cyclodialysis spatula for glaucoma surgery with a fiber-optics bundle incorporated within its thickness is described. The instrument is used as is commonly done at present with the standard cyclodialysis spatula. In addition, the fiber-optics bundle delivers cold light to the tip of the spatula which is useful in helping the surgeon locate the spatula within the sclera and the anterior chamber.", "contents": "A fiber-optics cyclodialysis spatula. A cyclodialysis spatula for glaucoma surgery with a fiber-optics bundle incorporated within its thickness is described. The instrument is used as is commonly done at present with the standard cyclodialysis spatula. In addition, the fiber-optics bundle delivers cold light to the tip of the spatula which is useful in helping the surgeon locate the spatula within the sclera and the anterior chamber."}
{"id": "PMID:460810", "title": "Vitrectomy in experimental endophthalmitis: Part I-Fungal infection.", "content": "In studying the dose-related ocular toxicity of amphotericin B in vitrectomized eyes, up to 5 micrograms/0.1 ml concentration of amphotericin C was nontoxic to the ocular structure when injected in the anterior part of the vitreous cavity. Intravitreal injection of 2.5 micrograms of amphotericin B halted fungal endophthalmitis, which was experimentally induced in rabbit eyes, even after 16 days of infection. Combined treatment of vitrectomy and intravitreal injection of amphotericin B not only cured the fungal endophthalmitis but it also cleared the ocular media from opacities.", "contents": "Vitrectomy in experimental endophthalmitis: Part I-Fungal infection. In studying the dose-related ocular toxicity of amphotericin B in vitrectomized eyes, up to 5 micrograms/0.1 ml concentration of amphotericin C was nontoxic to the ocular structure when injected in the anterior part of the vitreous cavity. Intravitreal injection of 2.5 micrograms of amphotericin B halted fungal endophthalmitis, which was experimentally induced in rabbit eyes, even after 16 days of infection. Combined treatment of vitrectomy and intravitreal injection of amphotericin B not only cured the fungal endophthalmitis but it also cleared the ocular media from opacities."}
{"id": "PMID:460811", "title": "Vitrectomy in experimental endophthalmitis: Part II--Bacterial endophthalmitis.", "content": "Three methods of treatment for Staphylococcus aureus endophthalmitis were compared: subconjunctival injection with intramuscular gentamicin, intravitreal injection with intramuscular gentamicin, and vitrectomy using infusion solution containing gentamicin along with intramuscular gentamicin. Subconjunctival injection with intramuscular gentamicin was not successful in treating infection. Intravitreal injection with intramuscular gentamicin and vitrectomy with intramuscular gentamicin were equally effective in eradicating infection; however, the combination of vitrectomy and intramuscular gentamicin provided clearer vitreous with greater fundus detail.", "contents": "Vitrectomy in experimental endophthalmitis: Part II--Bacterial endophthalmitis. Three methods of treatment for Staphylococcus aureus endophthalmitis were compared: subconjunctival injection with intramuscular gentamicin, intravitreal injection with intramuscular gentamicin, and vitrectomy using infusion solution containing gentamicin along with intramuscular gentamicin. Subconjunctival injection with intramuscular gentamicin was not successful in treating infection. Intravitreal injection with intramuscular gentamicin and vitrectomy with intramuscular gentamicin were equally effective in eradicating infection; however, the combination of vitrectomy and intramuscular gentamicin provided clearer vitreous with greater fundus detail."}
{"id": "PMID:460812", "title": "PHPV in an adult managed by vitrectomy.", "content": "Pars plana vitrectomy requiring a two-instrument technique successfully cleared the pupillary axis of dense persistent hyperplastic primary vitreous in a 29-year-old patient. To find PHPV in an adult eye with vision is so unusual that distinguishing it from the ocular sequelae of traumatic injury can be challenging. Hyperplastic primary vitreous persists with a wide variety of clinical features in both anterior and posterior segments of the eye.", "contents": "PHPV in an adult managed by vitrectomy. Pars plana vitrectomy requiring a two-instrument technique successfully cleared the pupillary axis of dense persistent hyperplastic primary vitreous in a 29-year-old patient. To find PHPV in an adult eye with vision is so unusual that distinguishing it from the ocular sequelae of traumatic injury can be challenging. Hyperplastic primary vitreous persists with a wide variety of clinical features in both anterior and posterior segments of the eye."}
{"id": "PMID:460814", "title": "Sickled erythrocytes, hyphema, and secondary glaucoma: I. The diagnosis and treatment of sickled erythrocytes in human hyphemas.", "content": "Four patients with sickle cell hemoglobinopathies (one SC, three AS) and hyphemas were found to have more erythrocytes sickled in their anterior chambers than in their circulating venous blood. Intraocular pressure was severely elevated, despite relatively small amounts of intracameral blood. Systemic hypotensive agents were not always successful in reducing IOP, and in patients with sickle cell hemoglobinopathy, are probably contraindicated in high or repeated dose regimens. Moderate elevation of IOP in sickle cell hemoglobinopathy patients may produce rapid deterioration of visual function, perhaps because of a greater than usual effect on vascular perfusion in the central retinal artery and optic nerve. Early anterior chamber paracentesis may be the best treatment for this type of hyphema-induced secondary glaucoma.", "contents": "Sickled erythrocytes, hyphema, and secondary glaucoma: I. The diagnosis and treatment of sickled erythrocytes in human hyphemas. Four patients with sickle cell hemoglobinopathies (one SC, three AS) and hyphemas were found to have more erythrocytes sickled in their anterior chambers than in their circulating venous blood. Intraocular pressure was severely elevated, despite relatively small amounts of intracameral blood. Systemic hypotensive agents were not always successful in reducing IOP, and in patients with sickle cell hemoglobinopathy, are probably contraindicated in high or repeated dose regimens. Moderate elevation of IOP in sickle cell hemoglobinopathy patients may produce rapid deterioration of visual function, perhaps because of a greater than usual effect on vascular perfusion in the central retinal artery and optic nerve. Early anterior chamber paracentesis may be the best treatment for this type of hyphema-induced secondary glaucoma."}
{"id": "PMID:460815", "title": "Sickled erythrocytes, hyphema, and secondary glaucoma: III. Effects of sicle cell and normal human blood samples in rabbit anterior chambers.", "content": "When injected into rabbit anterior chambers, human sickle cell erythrocytes cause more intense and prolonged effects than do nonsickling erythrocytes. The consequences of injected sickle cell trait erythrocytes are not less severe than those of injected SC, SS, and Sthal erythrocytes. Regardless of hemoglobin composition, blood with a higher hematocrit value causes more severe complications than blood with a lower hematocrit value.", "contents": "Sickled erythrocytes, hyphema, and secondary glaucoma: III. Effects of sicle cell and normal human blood samples in rabbit anterior chambers. When injected into rabbit anterior chambers, human sickle cell erythrocytes cause more intense and prolonged effects than do nonsickling erythrocytes. The consequences of injected sickle cell trait erythrocytes are not less severe than those of injected SC, SS, and Sthal erythrocytes. Regardless of hemoglobin composition, blood with a higher hematocrit value causes more severe complications than blood with a lower hematocrit value."}
{"id": "PMID:460816", "title": "Sickled erythrocytes, hyphema, and secondary glaucoma: IV. The rate and percentage of sickling of erythrocytes in rabbit aqueous humor, in vitro and in vivo.", "content": "The number of erythrocytes adopting the sickled configuration in rabbit aqueous was determined with a masked counting technique in vitro and in vivo. The percentage of cells that became sickled in aqueous humor was greater than that observed in a control salt solution and in the donor blood itself. Aqueous humor, therefore, can be considered a particularly deleterious medium for erythrocytes having a propensity for sickling. Blood samples from six donors with systemically severe hemoglobinopathies (SC, Sthal, and SS) had relatively high levels of hemoglobin S and, in general, showed faster and more frequent sickling than did blood samples from donors with low levels of hemoglobin S (one SS patient, whose chronic blood transfusion regimen had replaced most of his hemoglobin S with normal hemoglobin, and two AS patients). Even erythrocytes from sickle cell trait cases, however, were capable of sickling when immersed in acqueous humor.", "contents": "Sickled erythrocytes, hyphema, and secondary glaucoma: IV. The rate and percentage of sickling of erythrocytes in rabbit aqueous humor, in vitro and in vivo. The number of erythrocytes adopting the sickled configuration in rabbit aqueous was determined with a masked counting technique in vitro and in vivo. The percentage of cells that became sickled in aqueous humor was greater than that observed in a control salt solution and in the donor blood itself. Aqueous humor, therefore, can be considered a particularly deleterious medium for erythrocytes having a propensity for sickling. Blood samples from six donors with systemically severe hemoglobinopathies (SC, Sthal, and SS) had relatively high levels of hemoglobin S and, in general, showed faster and more frequent sickling than did blood samples from donors with low levels of hemoglobin S (one SS patient, whose chronic blood transfusion regimen had replaced most of his hemoglobin S with normal hemoglobin, and two AS patients). Even erythrocytes from sickle cell trait cases, however, were capable of sickling when immersed in acqueous humor."}
{"id": "PMID:460817", "title": "Sickled erythrocytes, hyphema, and secondary glaucoma: V. The effect of vitamin C on erythrocyte sickling in aqueous humor.", "content": "In vivo incubations of Hgb SC erythrocytes showed significantly more sickling in anterior chambers characterized by high concentrations of ascorbic acid than in anterior chambers with low concentrations of ascorbic acid (normal guinea pigs compared with scorbutic guinea pigs and normal guinea pigs compared with normal rats). Low concentrations of ascorbic acid, however, did not completely eliminate intracameral sickling. Because acetazolamide raises the concentration of aqueous humor ascorbate, it should be used with considerable discretion when treating hyphema and secondary glaucoma caused by sickle cells. Methazolamide may be more desirable.", "contents": "Sickled erythrocytes, hyphema, and secondary glaucoma: V. The effect of vitamin C on erythrocyte sickling in aqueous humor. In vivo incubations of Hgb SC erythrocytes showed significantly more sickling in anterior chambers characterized by high concentrations of ascorbic acid than in anterior chambers with low concentrations of ascorbic acid (normal guinea pigs compared with scorbutic guinea pigs and normal guinea pigs compared with normal rats). Low concentrations of ascorbic acid, however, did not completely eliminate intracameral sickling. Because acetazolamide raises the concentration of aqueous humor ascorbate, it should be used with considerable discretion when treating hyphema and secondary glaucoma caused by sickle cells. Methazolamide may be more desirable."}
{"id": "PMID:460818", "title": "Composition and evaluation of the efficacy of Staphylococcus aureus vaccine.", "content": "An alum-precipitated Staphylococcus aureus vaccine, composed of a formalin-inactivated whole culture of a strain which produces Smith surface antigen and combined with the whole culture of a highly toxigenic strain, was found to afford a good immunity to staphylococcal skin infection in rabbits. Three injections of the vaccine provided immunity which lasted for at least 6 months against a primarily pyogenic strain of S. aureus and for at least 3 months against a toxigenic strain. From experiments using vaccines prepared from cells or toxoid only, it was deduced that, although there is a measure of strain specific immunity, a good heterologous immunity can be established with a combined product provided that it contains adequate quantities of toxoid. The use of such a vaccine as a potential aid in the control of bovine staphylococcal mastitis is discussed.", "contents": "Composition and evaluation of the efficacy of Staphylococcus aureus vaccine. An alum-precipitated Staphylococcus aureus vaccine, composed of a formalin-inactivated whole culture of a strain which produces Smith surface antigen and combined with the whole culture of a highly toxigenic strain, was found to afford a good immunity to staphylococcal skin infection in rabbits. Three injections of the vaccine provided immunity which lasted for at least 6 months against a primarily pyogenic strain of S. aureus and for at least 3 months against a toxigenic strain. From experiments using vaccines prepared from cells or toxoid only, it was deduced that, although there is a measure of strain specific immunity, a good heterologous immunity can be established with a combined product provided that it contains adequate quantities of toxoid. The use of such a vaccine as a potential aid in the control of bovine staphylococcal mastitis is discussed."}
{"id": "PMID:460819", "title": "Aerospora of an Eragrostis curvula pasture in South Africa.", "content": "A qualitative and quantitative survey was made of the aerospora above an Eragrostis curvula (Schrad.) Nees pasture. The pasture, which lay adjacent to a field of Panicum coloratum L. where an outbread of ovine photosensitivity had occurred, was not grazed during the survey. A Burkard volumetric spore trap was used to sample the aerospora continuously for 10 months. Twenty components of the aerospora were recognized: spores of 9 genera of fungi (Cladosporium, Alternaria, Epicoccum, Drechslera, Pithomyces chartarum (Berk. & Curt.) M. B. Ellis, Nigrospora, Sporormia, Curvularia, Spegazzinia); 8 recognizable but unidentified spore types; hyphal fragments and pollen grains. Cladosporium conidia comprised 83,37% of the total aerospora. Conidia of Pithomyces chartarum formed a small (0,29%) but significant component of the aerospora, since this fungus is known to cause hepatogenous photosensitivity.", "contents": "Aerospora of an Eragrostis curvula pasture in South Africa. A qualitative and quantitative survey was made of the aerospora above an Eragrostis curvula (Schrad.) Nees pasture. The pasture, which lay adjacent to a field of Panicum coloratum L. where an outbread of ovine photosensitivity had occurred, was not grazed during the survey. A Burkard volumetric spore trap was used to sample the aerospora continuously for 10 months. Twenty components of the aerospora were recognized: spores of 9 genera of fungi (Cladosporium, Alternaria, Epicoccum, Drechslera, Pithomyces chartarum (Berk. & Curt.) M. B. Ellis, Nigrospora, Sporormia, Curvularia, Spegazzinia); 8 recognizable but unidentified spore types; hyphal fragments and pollen grains. Cladosporium conidia comprised 83,37% of the total aerospora. Conidia of Pithomyces chartarum formed a small (0,29%) but significant component of the aerospora, since this fungus is known to cause hepatogenous photosensitivity."}
{"id": "PMID:460820", "title": "Parasites of domestic and wild animals in South Africa. XII. Artificial transmission of nematodes from blesbok and impala to sheep, goats and cattle.", "content": "Sheep were successfully infested artificially with the larvae of Haemonchus contortus, Trichostrongylus axei, Trichostrongylus falculatus and Impalaja nudicollis, cultured from the faeces of blesbok naturally infested with these nematodes. Haemonchus placei, Longistrongylus sabie, Trichostrongylus colubrifomis, T. falculatus, Impalaia tuberculata and Cooperia hungi likewise became established in sheep, goats and calves dosed with larvae cultured from the faeces of infested impala. Sheep and goats could also be infested with Cooperioides hamiltoni and Oesophagostomum columbianum of impala origin, but Cooperioides hepaticae could not be transmitted either to these hosts or to calves.", "contents": "Parasites of domestic and wild animals in South Africa. XII. Artificial transmission of nematodes from blesbok and impala to sheep, goats and cattle. Sheep were successfully infested artificially with the larvae of Haemonchus contortus, Trichostrongylus axei, Trichostrongylus falculatus and Impalaja nudicollis, cultured from the faeces of blesbok naturally infested with these nematodes. Haemonchus placei, Longistrongylus sabie, Trichostrongylus colubrifomis, T. falculatus, Impalaia tuberculata and Cooperia hungi likewise became established in sheep, goats and calves dosed with larvae cultured from the faeces of infested impala. Sheep and goats could also be infested with Cooperioides hamiltoni and Oesophagostomum columbianum of impala origin, but Cooperioides hepaticae could not be transmitted either to these hosts or to calves."}
{"id": "PMID:460821", "title": "Parasites of domestic and wild animals in South Africa. XIII. The seasonal incidence of adult ticks (Acarina: Ixodidae) on cattle in the Northern Transvaal.", "content": "Ticks were collected over a period of 13 months, at approximately fortnightly intervals, from 6 Africaner oxen kept on the farm Nylsvley in the Naboomspruit District. The 8 species of Ixodidae collected were, in order of abundance, Rhipicephalus appendiculatus, R. evertsi evertsi, Hyalomma marginatum rufipes, H. truncatum, Amblyomma hebraeum, R. simus, Boophilus decoloratus and Ixodes cavipalpus. The relative abundance of adult ticks of these species, their predilection feeding sites and seasonal fluctuations in numbers are discussed.", "contents": "Parasites of domestic and wild animals in South Africa. XIII. The seasonal incidence of adult ticks (Acarina: Ixodidae) on cattle in the Northern Transvaal. Ticks were collected over a period of 13 months, at approximately fortnightly intervals, from 6 Africaner oxen kept on the farm Nylsvley in the Naboomspruit District. The 8 species of Ixodidae collected were, in order of abundance, Rhipicephalus appendiculatus, R. evertsi evertsi, Hyalomma marginatum rufipes, H. truncatum, Amblyomma hebraeum, R. simus, Boophilus decoloratus and Ixodes cavipalpus. The relative abundance of adult ticks of these species, their predilection feeding sites and seasonal fluctuations in numbers are discussed."}
{"id": "PMID:460822", "title": "An electron microscopic study of intra-erythrocytic stages of Babesia bovis in the brain capillaries of infected splenectomized calves.", "content": "Splenectomized vaccine donor calves undergoing primary reactions to Babesia bovis infections may develop cerebral babesiosis which leads to death if not treated in time. A brain biopsy was performed on an artificially-infected animal showing nervous symptoms and the tissue was immediately processed for electron microscopic examination. Virtually every erythrocyte in the brain capillaries sectioned was infected with B. bovis. Intra-erythrocytic merozoites, trophozoites and dividing trophozoites were indentified. Important features of the piriform merozoites included a reduced apical complex consisting of the anterior polar ring, microtubules, rhoptries and micronemes. Unidentified membrane-bound bodies, mostly spherical in shape, were observed anterior to the nucleus. The trophozoites showed very little structural differentiation and no food vacuoles or micropores could be detected. Each trophozoite produced 2 identical merozoites and the parent cell became totally incorporated in the daughter merozoites in the multiplication process. Projections were seen radiating from the surface of infected erythrocytes which appeared to adhere to other surfaces on contact. This probably resulted in the sludging of infected erythrocytes in the capillaries. The latter observations coincide with those described for Babesia argentina.", "contents": "An electron microscopic study of intra-erythrocytic stages of Babesia bovis in the brain capillaries of infected splenectomized calves. Splenectomized vaccine donor calves undergoing primary reactions to Babesia bovis infections may develop cerebral babesiosis which leads to death if not treated in time. A brain biopsy was performed on an artificially-infected animal showing nervous symptoms and the tissue was immediately processed for electron microscopic examination. Virtually every erythrocyte in the brain capillaries sectioned was infected with B. bovis. Intra-erythrocytic merozoites, trophozoites and dividing trophozoites were indentified. Important features of the piriform merozoites included a reduced apical complex consisting of the anterior polar ring, microtubules, rhoptries and micronemes. Unidentified membrane-bound bodies, mostly spherical in shape, were observed anterior to the nucleus. The trophozoites showed very little structural differentiation and no food vacuoles or micropores could be detected. Each trophozoite produced 2 identical merozoites and the parent cell became totally incorporated in the daughter merozoites in the multiplication process. Projections were seen radiating from the surface of infected erythrocytes which appeared to adhere to other surfaces on contact. This probably resulted in the sludging of infected erythrocytes in the capillaries. The latter observations coincide with those described for Babesia argentina."}
{"id": "PMID:460823", "title": "A bioassay method for the pheromone(s) of the bont tick Amblyomma hebraeum Koch.", "content": "A bioassay was developed which enabled the detection of the pheromones of the bont tick (Amblyomma hebraeum Koch) within 2 hours with a reproducibility of 82 +/- 13%. Dried, solvent-extracted ticks were glued onto the shaved backs of rabbits. Those ticks to which pheromone extract was subsequently applied strongly attracted females of the same species. In a 2-way choice test a cork disc impregnated with pheromone extract was even more attractive to female ticks than a treated dried tick.", "contents": "A bioassay method for the pheromone(s) of the bont tick Amblyomma hebraeum Koch. A bioassay was developed which enabled the detection of the pheromones of the bont tick (Amblyomma hebraeum Koch) within 2 hours with a reproducibility of 82 +/- 13%. Dried, solvent-extracted ticks were glued onto the shaved backs of rabbits. Those ticks to which pheromone extract was subsequently applied strongly attracted females of the same species. In a 2-way choice test a cork disc impregnated with pheromone extract was even more attractive to female ticks than a treated dried tick."}
{"id": "PMID:460824", "title": "The serological relationship of herpesvirus ovis to other herpesviruses and its possible involvement in the aetiology of jaagsiekte.", "content": "Cross-neutralization studies showed that 3 different isolates of herpesvirus ovis from cell cultures derived from the lungs of sheep suffering from jaagsiekte were not only identical but were also related to similar isolate made in Scotland. No relationship, however, could be established between herpesvirus ovis and common bovine or equine herpesviruses. Antibodies to herpesvirus ovis were present in roughly 70% of all animals tested and no evidence was obtained for the involvement of the virus in the aetiology of jaagsiekte. On the other hand, the absence of antibodies in sheep sera from Iceland as well as the other data obtained in this study did not exclude involvement of the virus in jaagsiekte.", "contents": "The serological relationship of herpesvirus ovis to other herpesviruses and its possible involvement in the aetiology of jaagsiekte. Cross-neutralization studies showed that 3 different isolates of herpesvirus ovis from cell cultures derived from the lungs of sheep suffering from jaagsiekte were not only identical but were also related to similar isolate made in Scotland. No relationship, however, could be established between herpesvirus ovis and common bovine or equine herpesviruses. Antibodies to herpesvirus ovis were present in roughly 70% of all animals tested and no evidence was obtained for the involvement of the virus in the aetiology of jaagsiekte. On the other hand, the absence of antibodies in sheep sera from Iceland as well as the other data obtained in this study did not exclude involvement of the virus in jaagsiekte."}
{"id": "PMID:460825", "title": "Lymph node pathology in Trypanosoma brucei-infected sheep.", "content": "The temporal sequence of morphological changes occurring in the prefemoral lymph nodes of sheep experimentally infected with Trypanosoma brucei was studied at weekly intervals during the course of infection. Histopathological changes started with lymphoid stimulation which was soon overtaken and masked by intense proliferation of reticulo-endothelial cells. Reticulo-endothelial proliferation resulted in macrophage accumulation, some vascular damage and lymph stasis, varying from insidious to extensive. Reticulo-endothelial activity subsequently decreased and there was an upsurge of proliferative activity in lymphoid tissue. Lymphoid stimulation resulted in increased numbers of primary follicles with active germinal centres and produced cells primarily lymphoblast/plasmablast in type. The response appeared to involve blast cells of the germinal centres rather than thymus-dependent paracortical areas. Both reticulo-endothelial and lymphoid components exhibited marked cellular necrosis which increased throughout the course of infection. Intense lymphopoiesis was not correlated with lymphocytosis but was associated with lymphopaenia.", "contents": "Lymph node pathology in Trypanosoma brucei-infected sheep. The temporal sequence of morphological changes occurring in the prefemoral lymph nodes of sheep experimentally infected with Trypanosoma brucei was studied at weekly intervals during the course of infection. Histopathological changes started with lymphoid stimulation which was soon overtaken and masked by intense proliferation of reticulo-endothelial cells. Reticulo-endothelial proliferation resulted in macrophage accumulation, some vascular damage and lymph stasis, varying from insidious to extensive. Reticulo-endothelial activity subsequently decreased and there was an upsurge of proliferative activity in lymphoid tissue. Lymphoid stimulation resulted in increased numbers of primary follicles with active germinal centres and produced cells primarily lymphoblast/plasmablast in type. The response appeared to involve blast cells of the germinal centres rather than thymus-dependent paracortical areas. Both reticulo-endothelial and lymphoid components exhibited marked cellular necrosis which increased throughout the course of infection. Intense lymphopoiesis was not correlated with lymphocytosis but was associated with lymphopaenia."}
{"id": "PMID:460828", "title": "Correlation of arthroscopy with other diagnostic modalities.", "content": "In our experience arthroscopy proved to be the most reliable method of evaluating internal derangement of the knee. Among 96 patients who underwent arthrotomy, the arthroscopic diagnosis was confirmed in 96 per cent. The combined anteromedial, anterolateral, and posteromedial approaches consistently demonstrated all intraarticular disorders. We found the posterolateral approach of little benefit.", "contents": "Correlation of arthroscopy with other diagnostic modalities. In our experience arthroscopy proved to be the most reliable method of evaluating internal derangement of the knee. Among 96 patients who underwent arthrotomy, the arthroscopic diagnosis was confirmed in 96 per cent. The combined anteromedial, anterolateral, and posteromedial approaches consistently demonstrated all intraarticular disorders. We found the posterolateral approach of little benefit."}
{"id": "PMID:460829", "title": "Correlations of arthrography with arthroscopy.", "content": "Arthrography and arthroscopy were compared on a prospective basis in 100 consecutive and unselected knees, all in patients who underwent arthrography, arthroscopy, and surgery. Arthroscopy was found to be more accurate than arthrography in the diagnosis of medial and lateral meniscus and anterior cruciate ligament lesions. Because of its easy availability and relatively low cost, arthrography continues to be a useful extension of the physical examination of the knee, serving as a further diagnostic screening technique. However, arthroscopy is recommended in all patients prior to surgery because it provides more complete and more accurate delineation of intra-articular lesions.", "contents": "Correlations of arthrography with arthroscopy. Arthrography and arthroscopy were compared on a prospective basis in 100 consecutive and unselected knees, all in patients who underwent arthrography, arthroscopy, and surgery. Arthroscopy was found to be more accurate than arthrography in the diagnosis of medial and lateral meniscus and anterior cruciate ligament lesions. Because of its easy availability and relatively low cost, arthrography continues to be a useful extension of the physical examination of the knee, serving as a further diagnostic screening technique. However, arthroscopy is recommended in all patients prior to surgery because it provides more complete and more accurate delineation of intra-articular lesions."}
{"id": "PMID:460830", "title": "Arthroscopic visualization of the posteromedial compartment of the knee joint.", "content": "Introduction of the arthroscope in the midline through the patellar tendon about 1 cm. below the apex of the patella is described. The posterior compartments of the knee joint can be examined from this single entry. The technique has been used in 1232 patients without any complications. In 127 patients subjected also to arthrotomy the arthroscopic diagnosis proved to be correct in all instances except for one missed rupture of the posterior cruciate liagment. In that case a bucket handle rupture of the medial meniscus prevented the entry into the posteromedial compartment.", "contents": "Arthroscopic visualization of the posteromedial compartment of the knee joint. Introduction of the arthroscope in the midline through the patellar tendon about 1 cm. below the apex of the patella is described. The posterior compartments of the knee joint can be examined from this single entry. The technique has been used in 1232 patients without any complications. In 127 patients subjected also to arthrotomy the arthroscopic diagnosis proved to be correct in all instances except for one missed rupture of the posterior cruciate liagment. In that case a bucket handle rupture of the medial meniscus prevented the entry into the posteromedial compartment."}
{"id": "PMID:460832", "title": "Debris observed by arthroscopy of the knee.", "content": "In examination of 732 knee joints through the arthroscope since 1961, various types of debris were observed in 47. The debris was classified into four groups: precipitation of fibrin, degeneration and necrosis of villi, desquamation of articular cartilage, and metaplasia of villi. Diseases of the knee joint and their pathogenesis are discussed in the light of these findings.", "contents": "Debris observed by arthroscopy of the knee. In examination of 732 knee joints through the arthroscope since 1961, various types of debris were observed in 47. The debris was classified into four groups: precipitation of fibrin, degeneration and necrosis of villi, desquamation of articular cartilage, and metaplasia of villi. Diseases of the knee joint and their pathogenesis are discussed in the light of these findings."}
{"id": "PMID:460833", "title": "A method of arthroscopic surgery. Clinical evaluation of synovectomy with the electric resectoscope and removal of loose bodies in the knee joint.", "content": "The structure and function of the electric resectoscope have been discussed and the application to synovectomy and removal of loose bodies in the knee joint. Our clinical results have also been reported. The advantages and problems of synovectomy and arthroscopic surgery with the electric resectoscope as used in the knee joint are discussed, especially in comparison with conventional resection of the anterior two-thirds of the synovial membrane. For intra-articular use in the knee joint the electric resectoscope used for transurethral resection in urology was modified to perform synovectomy and arthroscopic surgery. Clinical folow-up observation indicated that synovectomy performed with the electric resectoscope produced results almost comparable with those of conventional synovectomy. Arthroscopic surgery with the electric resectoscope is a new method. It is performed through a small skin incision with fewer untoward effects. The operation is relatively painless and offers more rapid recovery after surgery. A few complications were noted by this method. None were serious. There were two epsiodes of infection during the early use of this technique.", "contents": "A method of arthroscopic surgery. Clinical evaluation of synovectomy with the electric resectoscope and removal of loose bodies in the knee joint. The structure and function of the electric resectoscope have been discussed and the application to synovectomy and removal of loose bodies in the knee joint. Our clinical results have also been reported. The advantages and problems of synovectomy and arthroscopic surgery with the electric resectoscope as used in the knee joint are discussed, especially in comparison with conventional resection of the anterior two-thirds of the synovial membrane. For intra-articular use in the knee joint the electric resectoscope used for transurethral resection in urology was modified to perform synovectomy and arthroscopic surgery. Clinical folow-up observation indicated that synovectomy performed with the electric resectoscope produced results almost comparable with those of conventional synovectomy. Arthroscopic surgery with the electric resectoscope is a new method. It is performed through a small skin incision with fewer untoward effects. The operation is relatively painless and offers more rapid recovery after surgery. A few complications were noted by this method. None were serious. There were two epsiodes of infection during the early use of this technique."}
{"id": "PMID:460834", "title": "The effect of high tibial osteotomy on osteoarthritis of the knee. An arthroscopic study of 54 knee joints.", "content": "1. Arthroscopic findings in repair of articular surfaces and the operation's effect on healthy components made it clear that the ideal correction method is to align the mechanical axis to pass through a point 30 to 40 per cent lateral to the midpoint. 2. If ideal correction was obtained, it was observed by arthroscopy that repair of the ulcerated region was initiated by the surviving cartilage in the affected area and the cartilage bordering the affected area. 3. About one and one-half to two years after osteotomy it was observed that the ulcerated region was thoroughly covered with fibrous and membranous tissue. 4. In some cases in which correction was ideal, repair of the ruptured meniscus was observed arthroscopically.", "contents": "The effect of high tibial osteotomy on osteoarthritis of the knee. An arthroscopic study of 54 knee joints. 1. Arthroscopic findings in repair of articular surfaces and the operation's effect on healthy components made it clear that the ideal correction method is to align the mechanical axis to pass through a point 30 to 40 per cent lateral to the midpoint. 2. If ideal correction was obtained, it was observed by arthroscopy that repair of the ulcerated region was initiated by the surviving cartilage in the affected area and the cartilage bordering the affected area. 3. About one and one-half to two years after osteotomy it was observed that the ulcerated region was thoroughly covered with fibrous and membranous tissue. 4. In some cases in which correction was ideal, repair of the ruptured meniscus was observed arthroscopically."}
{"id": "PMID:460835", "title": "Arthroscopy in spontaneous osteonecrosis of the knee.", "content": "Eight knees with spontaneous osteonecrosis were investigated through arthroscopy, and in seven the intra-articular findings were rechecked at arthrotomy performed one to two weeks later. Anbormalities in the articular cartilage were observed in detail under arthroscopy that could not be seen in roentgenograms. In the early stage of osteonecrosis, flattening and fissures in the articular surfaces, with and without formation of a cartilage flap, were useful findings in choosing intra-articular surgical procedures. In the late stage, free bodies such as cartilage plates in the joint and regeneration with fibro-cartilaginous tissue over the necrotic lesion were also important in selecting surgical treatment. In addition to roentgenographic classification of developmental stages in osteonecrosis, arthroscopy is necessary in differentiating osteoarthroses, in observing the articular surface of the femoral condyles, and in determining the stage of disease when roentgenography provided insufficient information to justify intra-articular procedures such as drilling and bone grafting.", "contents": "Arthroscopy in spontaneous osteonecrosis of the knee. Eight knees with spontaneous osteonecrosis were investigated through arthroscopy, and in seven the intra-articular findings were rechecked at arthrotomy performed one to two weeks later. Anbormalities in the articular cartilage were observed in detail under arthroscopy that could not be seen in roentgenograms. In the early stage of osteonecrosis, flattening and fissures in the articular surfaces, with and without formation of a cartilage flap, were useful findings in choosing intra-articular surgical procedures. In the late stage, free bodies such as cartilage plates in the joint and regeneration with fibro-cartilaginous tissue over the necrotic lesion were also important in selecting surgical treatment. In addition to roentgenographic classification of developmental stages in osteonecrosis, arthroscopy is necessary in differentiating osteoarthroses, in observing the articular surface of the femoral condyles, and in determining the stage of disease when roentgenography provided insufficient information to justify intra-articular procedures such as drilling and bone grafting."}
{"id": "PMID:460836", "title": "Arthroscopic meniscectomy.", "content": "A technique is described for doing arthroscopic meniscectomies using short curved instruments passed through flexible, sealable Teflon sleeves. The goal, with few exceptions, was to achieve en bloc subtotal resection of the posterior two-thirds of the meniscus. The location and character of the lesions found in this series tend to support this pattern of excision. Immediate postoperative morbidity is astonishingly less than that seen with open meniscectomy. Economic losses, including medical costs and time off work, were significantly less, and short term results compare favorably with those of conventional meniscectomy in regard to relief of symptoms and restoration of function.", "contents": "Arthroscopic meniscectomy. A technique is described for doing arthroscopic meniscectomies using short curved instruments passed through flexible, sealable Teflon sleeves. The goal, with few exceptions, was to achieve en bloc subtotal resection of the posterior two-thirds of the meniscus. The location and character of the lesions found in this series tend to support this pattern of excision. Immediate postoperative morbidity is astonishingly less than that seen with open meniscectomy. Economic losses, including medical costs and time off work, were significantly less, and short term results compare favorably with those of conventional meniscectomy in regard to relief of symptoms and restoration of function."}
{"id": "PMID:460837", "title": "Meniscus surgery using the Watanabe arthroscope.", "content": "1. The present uses for arthroscopic meniscus surgery are explained. 2. Postoperative care is facilitated by arthroscopic meniscus surgery. However, improvement in surgical instruments and manipulation is necessary in order to complete the surgery in a short time and to make it easy to perform. 3. Total meniscectomy is especially useful in the Japanese because the discoid meniscus was observed in 16.6 per cent in my arthroscopic study. The incidence is considerably higher than that in foreigners. The procedure for arthroscopic total meniscectomy must be improved. 4. The three puncture method is useful. 5. Partial meniscectomy yields excellent results, but there are pitfalls to watch for. 6. A case of combined surgery with partial meniscectomy and meniscorisis is presented.", "contents": "Meniscus surgery using the Watanabe arthroscope. 1. The present uses for arthroscopic meniscus surgery are explained. 2. Postoperative care is facilitated by arthroscopic meniscus surgery. However, improvement in surgical instruments and manipulation is necessary in order to complete the surgery in a short time and to make it easy to perform. 3. Total meniscectomy is especially useful in the Japanese because the discoid meniscus was observed in 16.6 per cent in my arthroscopic study. The incidence is considerably higher than that in foreigners. The procedure for arthroscopic total meniscectomy must be improved. 4. The three puncture method is useful. 5. Partial meniscectomy yields excellent results, but there are pitfalls to watch for. 6. A case of combined surgery with partial meniscectomy and meniscorisis is presented."}
{"id": "PMID:460838", "title": "Peripheral detachment of the menisci of the knee: a preliminary report.", "content": "1. Anterior central detachments do satisfactorily as outlined. 2. However, if synovial meniscal detachments can be subluxed into the tibiofemoral junction, they should be sutured to their synovial ligamentous beds. 3. Both types of peripheral detachment coexist frequently with other intra-articular derangements, which are usually minor except when the anterior cruciate ligament is involved.", "contents": "Peripheral detachment of the menisci of the knee: a preliminary report. 1. Anterior central detachments do satisfactorily as outlined. 2. However, if synovial meniscal detachments can be subluxed into the tibiofemoral junction, they should be sutured to their synovial ligamentous beds. 3. Both types of peripheral detachment coexist frequently with other intra-articular derangements, which are usually minor except when the anterior cruciate ligament is involved."}
{"id": "PMID:460841", "title": "Arthroscopy in the evaluation of operative treatment of osteochondrosis dissecans.", "content": "In an early stage of postoperative evaluation of intra-articular surgery, as in osteochondrosis dissecans, arthroscopic examination is indispensable. In order to compare the results of different cases, a classification is necessary. The arthroscopic grading system presented is valuable for assessing degeneration of the three knee compartments. Early arthroscopic evaluation for follow-up can be used to classify postoperative complications that are not visable radiologically and are not severe enough to justify re-exploration. After follow-up examination with arthroscopy, advice to the patient may halt the progression of deterioration. Further study of the different treatments can help in formulating a plan of management of osteochondrosis dissecans.", "contents": "Arthroscopy in the evaluation of operative treatment of osteochondrosis dissecans. In an early stage of postoperative evaluation of intra-articular surgery, as in osteochondrosis dissecans, arthroscopic examination is indispensable. In order to compare the results of different cases, a classification is necessary. The arthroscopic grading system presented is valuable for assessing degeneration of the three knee compartments. Early arthroscopic evaluation for follow-up can be used to classify postoperative complications that are not visable radiologically and are not severe enough to justify re-exploration. After follow-up examination with arthroscopy, advice to the patient may halt the progression of deterioration. Further study of the different treatments can help in formulating a plan of management of osteochondrosis dissecans."}
{"id": "PMID:460842", "title": "The use of arthroscopy for follow-up in knee joint surgery.", "content": "Fifty-three joints in 48 patients were examined arthroscopically after surgery in order to evaluate postoperative results and particularly to reveal the causes of poor results. The chief arthroscopic findings in unsatisfactory cases were the following: recurrence of synovitis or osteoarthritic changes after synovectomy for rheumatoid arthritis, residual meniscal or osteoarthritic changes after meniscectomy, loose ligamentous or meniscal lesions after ligamentous repair or reconstruction, and poor regeneration of cartilage after high tibial osteotomy.", "contents": "The use of arthroscopy for follow-up in knee joint surgery. Fifty-three joints in 48 patients were examined arthroscopically after surgery in order to evaluate postoperative results and particularly to reveal the causes of poor results. The chief arthroscopic findings in unsatisfactory cases were the following: recurrence of synovitis or osteoarthritic changes after synovectomy for rheumatoid arthritis, residual meniscal or osteoarthritic changes after meniscectomy, loose ligamentous or meniscal lesions after ligamentous repair or reconstruction, and poor regeneration of cartilage after high tibial osteotomy."}
{"id": "PMID:460844", "title": "Pain in the knee in children: the medial plica shelf syndrome.", "content": "The medial plica shelf syndrome as a clinical entity should be added to the list of differential diagnoses as a possible cause of symptoms of internal derangement of the knee in children. It most frequently mimics chondromalacia of the patella and indeed may lead to it. It can be clinically suspected in the presence of the signs described in this report. The condition can be diagnosed early and accurately by arthroscopy and thereafter treated satisfactorily, with minimal morbidity. The results of such treatment not only have been gratifying over the period of follow-up (five to 32 months), but in all probability have prevented further serious damage to the knee.", "contents": "Pain in the knee in children: the medial plica shelf syndrome. The medial plica shelf syndrome as a clinical entity should be added to the list of differential diagnoses as a possible cause of symptoms of internal derangement of the knee in children. It most frequently mimics chondromalacia of the patella and indeed may lead to it. It can be clinically suspected in the presence of the signs described in this report. The condition can be diagnosed early and accurately by arthroscopy and thereafter treated satisfactorily, with minimal morbidity. The results of such treatment not only have been gratifying over the period of follow-up (five to 32 months), but in all probability have prevented further serious damage to the knee."}
{"id": "PMID:460845", "title": "Arthroscopy of the wrist and finger joints.", "content": "Ninety arthroscopies of the wrist and finger joints in 34 clinical cases and two amputated arms were carried out with the number 24 arthroscope. Eighty-four wrist and finger joints of four cadavers and two amputated arms were also dissected for macroscopic observation. Most portions of the interior structures of the wrist and finger joints can be observed by the dorsal approaches. Color photography and punch biopsy are also possible. Some arthroscopic photographs taken with the number 24 arthroscope are illustrated. From these experiences it is concluded that the number 24 arthroscope is a useful diagnostic tool in arthroscopy of the small joints, even though there are still many problems.", "contents": "Arthroscopy of the wrist and finger joints. Ninety arthroscopies of the wrist and finger joints in 34 clinical cases and two amputated arms were carried out with the number 24 arthroscope. Eighty-four wrist and finger joints of four cadavers and two amputated arms were also dissected for macroscopic observation. Most portions of the interior structures of the wrist and finger joints can be observed by the dorsal approaches. Color photography and punch biopsy are also possible. Some arthroscopic photographs taken with the number 24 arthroscope are illustrated. From these experiences it is concluded that the number 24 arthroscope is a useful diagnostic tool in arthroscopy of the small joints, even though there are still many problems."}
{"id": "PMID:460846", "title": "Problems in recording arthroscopy.", "content": "Several good combinations of photographic equipment are available today for recording arthroscopies. Some of them are so simple to use that everyone can obtain good still pictures through the arthroscope. It is usually preferable to enlarge the arthroscopic pictures in order to get better slides for presentations during lectures and at scientific meetings. Movie film and video tape offer a more dynamic recording but are both fairly expensive. The use of video tape can cause problems during presentations to larger audiences. Video tape, however, can be transferred to 16 mm. movie film, which is the international standard in the teaching world. Color TV has three different standards in different parts of the world and there are at least four different video casette systems available today.", "contents": "Problems in recording arthroscopy. Several good combinations of photographic equipment are available today for recording arthroscopies. Some of them are so simple to use that everyone can obtain good still pictures through the arthroscope. It is usually preferable to enlarge the arthroscopic pictures in order to get better slides for presentations during lectures and at scientific meetings. Movie film and video tape offer a more dynamic recording but are both fairly expensive. The use of video tape can cause problems during presentations to larger audiences. Video tape, however, can be transferred to 16 mm. movie film, which is the international standard in the teaching world. Color TV has three different standards in different parts of the world and there are at least four different video casette systems available today."}
{"id": "PMID:460873", "title": "The initial evaluation of the patient with multiple injuries.", "content": "The management of trauma patients requires organization and the cooperation of trained health personnel, beginning at the site of injury and continuing until the patient is discharged from the hospital. Treatment guidelines and priorities must be well outlined in advance to provide optimal care and minimize delay prior to definitive therapy. Resuscitation and treatment must begin immediately and simultaneously following well established principles. These guidelines and the general care of the patient with multiple trauma are discussed in this article.", "contents": "The initial evaluation of the patient with multiple injuries. The management of trauma patients requires organization and the cooperation of trained health personnel, beginning at the site of injury and continuing until the patient is discharged from the hospital. Treatment guidelines and priorities must be well outlined in advance to provide optimal care and minimize delay prior to definitive therapy. Resuscitation and treatment must begin immediately and simultaneously following well established principles. These guidelines and the general care of the patient with multiple trauma are discussed in this article."}
{"id": "PMID:460874", "title": "What constitutes a neurosurgical emergency?", "content": "Because of the large numbers of head injuries, physicians are frequently called upon to evaluate and initiate treatment in these acute emergencies. Deterioration of such patients results from direct injury to the brain tissue and a subsequent increase in the intracranial pressure. The pathophysiology of brain injury is manifested in a cycle of detrimental events with increased tissue damage and progressive neurological deficit. Current concepts in management of head injuries aimed at interrupting this cycle of events are discussed in detail.", "contents": "What constitutes a neurosurgical emergency? Because of the large numbers of head injuries, physicians are frequently called upon to evaluate and initiate treatment in these acute emergencies. Deterioration of such patients results from direct injury to the brain tissue and a subsequent increase in the intracranial pressure. The pathophysiology of brain injury is manifested in a cycle of detrimental events with increased tissue damage and progressive neurological deficit. Current concepts in management of head injuries aimed at interrupting this cycle of events are discussed in detail."}
{"id": "PMID:460876", "title": "The initial evaluation in maxillofacial trauma.", "content": "Evaluation in maxillofacial trauma is primarily an extension of the art of the physical examination, with inspection, palpation, and evaluation of loss of normal function as the primary requisites. X-ray evaluation is critical, and best results are usually obtained by taking films as soon following the injury as possible, before opacification of the sinus with blood, swelling, or hematoma obscures critical bone landmarks. Primary reduction and surgical management of these fractures can usually be delayed for a reasonable period; however, the most accurate findings on both physical examination and x-ray studies are those obtained as soon as possible after the injury.", "contents": "The initial evaluation in maxillofacial trauma. Evaluation in maxillofacial trauma is primarily an extension of the art of the physical examination, with inspection, palpation, and evaluation of loss of normal function as the primary requisites. X-ray evaluation is critical, and best results are usually obtained by taking films as soon following the injury as possible, before opacification of the sinus with blood, swelling, or hematoma obscures critical bone landmarks. Primary reduction and surgical management of these fractures can usually be delayed for a reasonable period; however, the most accurate findings on both physical examination and x-ray studies are those obtained as soon as possible after the injury."}
{"id": "PMID:460877", "title": "The initial evaluation in ophthalmic injury.", "content": "The recognition and proper initial handling of the many types of ocular injury will result in increased preservation of eyesight and fewer complications. This presentation has attempted to provide some of the general principles of ocular examination as a guide to the physician in dealing with ocular trauma.", "contents": "The initial evaluation in ophthalmic injury. The recognition and proper initial handling of the many types of ocular injury will result in increased preservation of eyesight and fewer complications. This presentation has attempted to provide some of the general principles of ocular examination as a guide to the physician in dealing with ocular trauma."}
{"id": "PMID:460878", "title": "Trauma involving the air and food passages.", "content": "The management of trauma to the air and food passages has been reviewed briefly, with emphasis on the management of the acute problems based upon a thorough evaluation.", "contents": "Trauma involving the air and food passages. The management of trauma to the air and food passages has been reviewed briefly, with emphasis on the management of the acute problems based upon a thorough evaluation."}
{"id": "PMID:460879", "title": "Management of upper airway obstruction.", "content": "The tongue is the most common cause of upper airway obstruction, a situation seen most often in patients who are comatose or who have suffered cardiopulmonary arrest. Other common causes of upper airway obstruction include edema of the oropharynx and larynx, trauma, foreign body, and infection. The management of the patient with upper airway obstruction depends upon the cause of the obstruction, the training and skills of the rescuer, and the availability of adjuncts necessary to perform advanced airway techniques. In most cases, merely positioning the patient or performing one of the three maneuvers designed to elevate the tongue will open the airway of the comatose patient or the victim of cardiac arrest. In patients with suspected foreign body obstruction, abdominal or chest compression should be performed immediately, with digital extraction of the foreign body reserved for those in whom these maneuvers are unsuccessful. Most patients with obstruction secondary to edema, trauma, or infection can be managed initially with orotracheal or nasotracheal intubation. Intubation should be attempted prior to surgical management of the airway in most cases of upper airway obstruction. Occasionally, however, cricothyroidotomy or tracheostomy is necessary to establish an airway. The choice of technique depends primarily on the experience and skills of the rescuing physician or paramedic. In most cases, cricothyroidotomy is technically more simple and more easily performed than tracheotomy, especially for the physician who has not been trained in surgery or otolaryngology and for the nonphysician rescuer. No matter what the method employed in establishing an airway in a patient with upper airway obstruction, it must be performed quickly and a source of ventilation provided for the patient once the airway has been secured.", "contents": "Management of upper airway obstruction. The tongue is the most common cause of upper airway obstruction, a situation seen most often in patients who are comatose or who have suffered cardiopulmonary arrest. Other common causes of upper airway obstruction include edema of the oropharynx and larynx, trauma, foreign body, and infection. The management of the patient with upper airway obstruction depends upon the cause of the obstruction, the training and skills of the rescuer, and the availability of adjuncts necessary to perform advanced airway techniques. In most cases, merely positioning the patient or performing one of the three maneuvers designed to elevate the tongue will open the airway of the comatose patient or the victim of cardiac arrest. In patients with suspected foreign body obstruction, abdominal or chest compression should be performed immediately, with digital extraction of the foreign body reserved for those in whom these maneuvers are unsuccessful. Most patients with obstruction secondary to edema, trauma, or infection can be managed initially with orotracheal or nasotracheal intubation. Intubation should be attempted prior to surgical management of the airway in most cases of upper airway obstruction. Occasionally, however, cricothyroidotomy or tracheostomy is necessary to establish an airway. The choice of technique depends primarily on the experience and skills of the rescuing physician or paramedic. In most cases, cricothyroidotomy is technically more simple and more easily performed than tracheotomy, especially for the physician who has not been trained in surgery or otolaryngology and for the nonphysician rescuer. No matter what the method employed in establishing an airway in a patient with upper airway obstruction, it must be performed quickly and a source of ventilation provided for the patient once the airway has been secured."}
{"id": "PMID:460880", "title": "Acute labyrinthine disorders.", "content": "A patient seeking emergency treatment for a labyrinthine disorder is usually complaining of dizziness. The task at hand in the emergency room is, first, to rule out the truly life threatening emergent disorders, and then by means of a rapid but adequate history and physical examination to aid in the differentiation between central and peripheral etiologies. If the problem is central, and especially if there are signs of a true emergency, there should be no hesitation in sharing the responsibility for the care of this patient with a neurologist or neurosurgeon. If one is convinced that the problem is of a peripheral etiology, one most likely is dealing with Meniere's disease, vestibular neuronitis, benign paroxysmal positional vertigo, or true labyrinthitis. An acoustic tumor must always be considered. Appropriate laboratory and x-ray studies may be initiated and therapy begun. Although the emergency room evaluation can be inclusive and accurate, it is necessarily incomplete. There is no substitute for a more thorough history and physical examination under more relaxed conditions, complete audiometry, electronystagmography, polytomography, myelography, angiography, or whatever additional sophisticated studies are deemed necessary. If performed well, however, the emergency room encounter forms an invaluable foundation upon which further diagnostic and treatment decisions are based.", "contents": "Acute labyrinthine disorders. A patient seeking emergency treatment for a labyrinthine disorder is usually complaining of dizziness. The task at hand in the emergency room is, first, to rule out the truly life threatening emergent disorders, and then by means of a rapid but adequate history and physical examination to aid in the differentiation between central and peripheral etiologies. If the problem is central, and especially if there are signs of a true emergency, there should be no hesitation in sharing the responsibility for the care of this patient with a neurologist or neurosurgeon. If one is convinced that the problem is of a peripheral etiology, one most likely is dealing with Meniere's disease, vestibular neuronitis, benign paroxysmal positional vertigo, or true labyrinthitis. An acoustic tumor must always be considered. Appropriate laboratory and x-ray studies may be initiated and therapy begun. Although the emergency room evaluation can be inclusive and accurate, it is necessarily incomplete. There is no substitute for a more thorough history and physical examination under more relaxed conditions, complete audiometry, electronystagmography, polytomography, myelography, angiography, or whatever additional sophisticated studies are deemed necessary. If performed well, however, the emergency room encounter forms an invaluable foundation upon which further diagnostic and treatment decisions are based."}
{"id": "PMID:460881", "title": "Temporal bone trauma and facial paralysis.", "content": "Temporal bone trauma includes a wide range of injuries of greater and lesser severity, most of which require the immediate attention of an otolaryngologist. The most common temporal bone injuries encountered in an emergency room setting include longitudinal and transverse fractures of the temporal bone with and without facial paralysis, labyrinthine concussion, foreign bodies in the external canal, penetrating injuries via the external auditory canal, and pressure injuries, including otitic barotrauma, water skiing accidents, blows or slaps to the ear or side of the head, and severe impact noise. A brief but inclusive examination can be performed within the limitations necessarily imposed by an emergency room setting. Cranial nerve screening, balance testing, hearing, and vestibular evaluation can be accomplished with a considerable degree of accuracy. To a large extent, the long term result reflects the quality of the initial examination.", "contents": "Temporal bone trauma and facial paralysis. Temporal bone trauma includes a wide range of injuries of greater and lesser severity, most of which require the immediate attention of an otolaryngologist. The most common temporal bone injuries encountered in an emergency room setting include longitudinal and transverse fractures of the temporal bone with and without facial paralysis, labyrinthine concussion, foreign bodies in the external canal, penetrating injuries via the external auditory canal, and pressure injuries, including otitic barotrauma, water skiing accidents, blows or slaps to the ear or side of the head, and severe impact noise. A brief but inclusive examination can be performed within the limitations necessarily imposed by an emergency room setting. Cranial nerve screening, balance testing, hearing, and vestibular evaluation can be accomplished with a considerable degree of accuracy. To a large extent, the long term result reflects the quality of the initial examination."}
{"id": "PMID:460883", "title": "Management of acute ear infections.", "content": "The differential diagnosis between the usual acute otitis externa and acute suppurative otitis media is presented. Other important and sometimes frequently seen entities that present as infections in the ear are also discussed. It is emphasized that a patient complaining of ear pain with normal findings on examination must be more thoroughly investigated because of the possibility of a more ominous condition such as carcinoma of the pharynx.", "contents": "Management of acute ear infections. The differential diagnosis between the usual acute otitis externa and acute suppurative otitis media is presented. Other important and sometimes frequently seen entities that present as infections in the ear are also discussed. It is emphasized that a patient complaining of ear pain with normal findings on examination must be more thoroughly investigated because of the possibility of a more ominous condition such as carcinoma of the pharynx."}
{"id": "PMID:460884", "title": "Sinusitis as an emergency.", "content": "The manifestations of diseases of the paranasal sinuses that may present as acute emergency situations and the more common complications of these diseases are briefly reviewed with suggestions for initial evaluation therapy.", "contents": "Sinusitis as an emergency. The manifestations of diseases of the paranasal sinuses that may present as acute emergency situations and the more common complications of these diseases are briefly reviewed with suggestions for initial evaluation therapy."}
{"id": "PMID:460885", "title": "Evaluation and treatment of acute bleeding from the head and neck.", "content": "Three entities that can cause life threatening bleeding in the head and neck are described: epistaxis, post-tonsillectomy bleeding, and post-tracheostomy bleeding. It behooves all physicians operating in this area and emergency room physicians and personnel to be acquainted with the diagnosis and management of these situations.", "contents": "Evaluation and treatment of acute bleeding from the head and neck. Three entities that can cause life threatening bleeding in the head and neck are described: epistaxis, post-tonsillectomy bleeding, and post-tracheostomy bleeding. It behooves all physicians operating in this area and emergency room physicians and personnel to be acquainted with the diagnosis and management of these situations."}
{"id": "PMID:460916", "title": "[Experiences with autogenic training groups (author's transl)].", "content": "According to I. H. Schultz, \"Autogenic Training\" has helped many children with psychosomatic symptoms. The children's symptoms were obviously influenced by disturbed family-situations and so we invited both children and parents to the \"Autogenic Training\". The treatment was carried out in two parallel groups, 6 children, 3 parents and 3 mothers. The process and the \"outcome\" of ten group-sessions are described.", "contents": "[Experiences with autogenic training groups (author's transl)]. According to I. H. Schultz, \"Autogenic Training\" has helped many children with psychosomatic symptoms. The children's symptoms were obviously influenced by disturbed family-situations and so we invited both children and parents to the \"Autogenic Training\". The treatment was carried out in two parallel groups, 6 children, 3 parents and 3 mothers. The process and the \"outcome\" of ten group-sessions are described."}
{"id": "PMID:460917", "title": "[Pneumopericardium in newborns during artificial ventilation (author's transl)].", "content": "During a period of 4 years 5 cases of pneumopericardium were observed among 571 artificially ventilated early- and newborn babies. 4 patients died as result of cardiac tamponade, 3 of them before the pericardiocentesis could be performed. 1 infant survived by puncture of the pericardial cavity. Frequency, pathological mechanism, and diagnosis of the pneumopericardium including quick terapeutical measurements under which pericardiocentesis could be life saving, are discussed.", "contents": "[Pneumopericardium in newborns during artificial ventilation (author's transl)]. During a period of 4 years 5 cases of pneumopericardium were observed among 571 artificially ventilated early- and newborn babies. 4 patients died as result of cardiac tamponade, 3 of them before the pericardiocentesis could be performed. 1 infant survived by puncture of the pericardial cavity. Frequency, pathological mechanism, and diagnosis of the pneumopericardium including quick terapeutical measurements under which pericardiocentesis could be life saving, are discussed."}
{"id": "PMID:460918", "title": "[Cervical teratomas. Case report and review (author's transl)].", "content": "The finding of a cervical teratoma in a newborn gave rise to a new review of the 131 cases in infancy described in the literature. 13 of the 29 cases reported since 1966 were found in newborns, and a total of 25 cervical teratomas by the end of the first year. The clinical differential diagnosis against other cervical tumors is difficult. With one exception, all teratomas were benign. Because of dangerous respiratory complications and the increasing risk of malign degeneration with age, this tumor has to be removed as early as possible.", "contents": "[Cervical teratomas. Case report and review (author's transl)]. The finding of a cervical teratoma in a newborn gave rise to a new review of the 131 cases in infancy described in the literature. 13 of the 29 cases reported since 1966 were found in newborns, and a total of 25 cervical teratomas by the end of the first year. The clinical differential diagnosis against other cervical tumors is difficult. With one exception, all teratomas were benign. Because of dangerous respiratory complications and the increasing risk of malign degeneration with age, this tumor has to be removed as early as possible."}
{"id": "PMID:460919", "title": "[Frequency of Kongenital Adrenal Hyperplasia (author's transl)].", "content": "The frequency of homozygous congenital adrenal hyperplasia in Tyrol is found to be 1 : 8991, the gene-frequency for congenital adrenal hyperplasia 1 : 95 and the frequency of heterozygous congenital adrenal hyperplasia 1 : 48. Our data is compared on a numerical and statistical base with that in Z\u00fcrich and Munich with regard to the frequency of congenital adrenal hyperplasia, to its distribution with and without salt loss and to its sex-distribution. According to our study one may assume a frequency of homozygous congenital adrenal hyperplasia in Tyrol, Z\u00fcrich and Munich of 1 : 7000--10,000.", "contents": "[Frequency of Kongenital Adrenal Hyperplasia (author's transl)]. The frequency of homozygous congenital adrenal hyperplasia in Tyrol is found to be 1 : 8991, the gene-frequency for congenital adrenal hyperplasia 1 : 95 and the frequency of heterozygous congenital adrenal hyperplasia 1 : 48. Our data is compared on a numerical and statistical base with that in Z\u00fcrich and Munich with regard to the frequency of congenital adrenal hyperplasia, to its distribution with and without salt loss and to its sex-distribution. According to our study one may assume a frequency of homozygous congenital adrenal hyperplasia in Tyrol, Z\u00fcrich and Munich of 1 : 7000--10,000."}
{"id": "PMID:460920", "title": "Diagnosis of alkaptonuria in children.", "content": "Thirty-nine patiens with alkaptonuria diagnosed in childhood were studied with repeated detailed clinical and biochemical examinations. Darkening of the urine, although varying from time to time, was present in all. Pigmentary changes in axillary regions began to appear at 8--10 years with concomitant staining of underwear. Ear cartilage staining occurred in only 1, and none had scleral involvement or anthropathy. Dark brown to black staining of ear cerumen is present even in earliest childhood. This distinctive symptom in a child with no other ochronotic manifestations and even normal urine colour should alert one to possible alkaptonnuria. Other findings are described in detail.", "contents": "Diagnosis of alkaptonuria in children. Thirty-nine patiens with alkaptonuria diagnosed in childhood were studied with repeated detailed clinical and biochemical examinations. Darkening of the urine, although varying from time to time, was present in all. Pigmentary changes in axillary regions began to appear at 8--10 years with concomitant staining of underwear. Ear cartilage staining occurred in only 1, and none had scleral involvement or anthropathy. Dark brown to black staining of ear cerumen is present even in earliest childhood. This distinctive symptom in a child with no other ochronotic manifestations and even normal urine colour should alert one to possible alkaptonnuria. Other findings are described in detail."}
{"id": "PMID:460921", "title": "[Growth development of children suffering from chronic insufficiency of digestion under dietary therapy for several years (author's transl)].", "content": "83 children suffering from malabsorption and disorders of metabolism were investigated with regard to body growth and weight gain. Though at first the increase of weight in comparison to body height was more rapid, the norm was obtained by the time the children were starting school. The period of retardation depended on the severity of disease and the diet being strictly kept to. It is most important that the circumference of the head was normal, though the other proportions of the body were too small for the age. These findings are parallel with normal intellectual development.", "contents": "[Growth development of children suffering from chronic insufficiency of digestion under dietary therapy for several years (author's transl)]. 83 children suffering from malabsorption and disorders of metabolism were investigated with regard to body growth and weight gain. Though at first the increase of weight in comparison to body height was more rapid, the norm was obtained by the time the children were starting school. The period of retardation depended on the severity of disease and the diet being strictly kept to. It is most important that the circumference of the head was normal, though the other proportions of the body were too small for the age. These findings are parallel with normal intellectual development."}
{"id": "PMID:460924", "title": "[The effectiveness of the lead covering in roentgen studies of the pelvis].", "content": "This is a report on the protective covering of the infant's pelvis. Comparative measurements of two customary lead covers, phantom measurements and comparative measurements on infants were carried out. Results show that a properly applied lead cover with a correct lead value (at least the equivalent of 1 mm of lead) may be considered a very effective protection against irradiation, a method that reduces irradiation to the gonads by a factor of 100. The initial dosis is at the surface and, as a rule is below 1 mR. The dosis attacking the gonads is most likely within the micro-R-range. Thus we state for practical purposes that a lead protective cover is very effective in X-raying the infant pelvis--indeed it should be mandatory for every pelvic X-ray taken in infants.", "contents": "[The effectiveness of the lead covering in roentgen studies of the pelvis]. This is a report on the protective covering of the infant's pelvis. Comparative measurements of two customary lead covers, phantom measurements and comparative measurements on infants were carried out. Results show that a properly applied lead cover with a correct lead value (at least the equivalent of 1 mm of lead) may be considered a very effective protection against irradiation, a method that reduces irradiation to the gonads by a factor of 100. The initial dosis is at the surface and, as a rule is below 1 mR. The dosis attacking the gonads is most likely within the micro-R-range. Thus we state for practical purposes that a lead protective cover is very effective in X-raying the infant pelvis--indeed it should be mandatory for every pelvic X-ray taken in infants."}
{"id": "PMID:460925", "title": "[Immunosuppressive treatment of endogenous uveitis in childhood (author's transl)].", "content": "This report is about a seven year old boy suffering from endogenous uveitis of unknown etiology in both eyes. A local and systemical steroid therapy did not stop the progressive course. Therefore an immunosuppressive treatment with methotrexate was begun. After the second intravenous application of this antifolate an amelioration could be noted and after 6 week a normalisation of the visual acuity on the left eye and an improvement from 0.4 to 0.8 on the right eye was obtained. Immunosuppressive treatment was discontinued after a duration of 14 months; now 2 years later, the inital success has been maintained. The advantages of methotrexate in comparison to other immunosuppressive agents are discussed.", "contents": "[Immunosuppressive treatment of endogenous uveitis in childhood (author's transl)]. This report is about a seven year old boy suffering from endogenous uveitis of unknown etiology in both eyes. A local and systemical steroid therapy did not stop the progressive course. Therefore an immunosuppressive treatment with methotrexate was begun. After the second intravenous application of this antifolate an amelioration could be noted and after 6 week a normalisation of the visual acuity on the left eye and an improvement from 0.4 to 0.8 on the right eye was obtained. Immunosuppressive treatment was discontinued after a duration of 14 months; now 2 years later, the inital success has been maintained. The advantages of methotrexate in comparison to other immunosuppressive agents are discussed."}
{"id": "PMID:460927", "title": "An improved radiant heat algometer and its application to pain threshold measurements in man.", "content": "A radiant heat algometer system is described which enables the psychophysical and clinical study of cutaneous pain sensations and reactions by means of a precise, stabilized power source. In this system DC electric power consumption of a bulb is automatically controlled, and no calibration is necessary to be controlled frequently. Moreover, attention is drawn to the significance of using the blackened sticky tape and of the pre-heating procedure. The application of this algometer is also described for a pain threshold determination with the intensity and time methods.", "contents": "An improved radiant heat algometer and its application to pain threshold measurements in man. A radiant heat algometer system is described which enables the psychophysical and clinical study of cutaneous pain sensations and reactions by means of a precise, stabilized power source. In this system DC electric power consumption of a bulb is automatically controlled, and no calibration is necessary to be controlled frequently. Moreover, attention is drawn to the significance of using the blackened sticky tape and of the pre-heating procedure. The application of this algometer is also described for a pain threshold determination with the intensity and time methods."}
{"id": "PMID:460928", "title": "Deafferentation hypersensitivity in the rat after dorsal rhizotomy: a possible animal model of chronic pain.", "content": "Unilateral dorsal rhizotomies were done at the cervicothoracic and lumbosacral spinal cord levels in rats. In preliminary experiments dermatome maps were determined for the roots to be sectioned. The behavior of 37 rats was observed for many months after the rhizotomies. The rats with the dorsal roots sectioned in the cervicothoracic spinal cord exhibited the following behavior: at the border of the skin adjacent to the zone of deafferentation, the rat scratched vigorously and progressively denuded the skin; self-mutilation of varying degrees occurred in the deafferented limb. In some animals scratching occurred in the contralateral skin dermatome opposite to the partially deafferented zone. The rats with the dorsal roots sectioned at the lumbosacral level exhibited hypersensitivity to cutaneous stimulation but there was no scratching or self-mutilation. These results are discussed in the light of previous similar research.", "contents": "Deafferentation hypersensitivity in the rat after dorsal rhizotomy: a possible animal model of chronic pain. Unilateral dorsal rhizotomies were done at the cervicothoracic and lumbosacral spinal cord levels in rats. In preliminary experiments dermatome maps were determined for the roots to be sectioned. The behavior of 37 rats was observed for many months after the rhizotomies. The rats with the dorsal roots sectioned in the cervicothoracic spinal cord exhibited the following behavior: at the border of the skin adjacent to the zone of deafferentation, the rat scratched vigorously and progressively denuded the skin; self-mutilation of varying degrees occurred in the deafferented limb. In some animals scratching occurred in the contralateral skin dermatome opposite to the partially deafferented zone. The rats with the dorsal roots sectioned at the lumbosacral level exhibited hypersensitivity to cutaneous stimulation but there was no scratching or self-mutilation. These results are discussed in the light of previous similar research."}
{"id": "PMID:460933", "title": "On the relation of injury to pain. The John J. Bonica lecture.", "content": "Pain is better classified as an awareness of a need-state than as a sensation. It serves more to promote healing than to avoid injury. It has more in common with the phenomena of hunger and thirst than it has with seeing or hearing. The period after injury is divided into the immediate, acute and chronic stages. In each stage it is shown that pain has only a weak connection to injury but a strong connection to the body state.", "contents": "On the relation of injury to pain. The John J. Bonica lecture. Pain is better classified as an awareness of a need-state than as a sensation. It serves more to promote healing than to avoid injury. It has more in common with the phenomena of hunger and thirst than it has with seeing or hearing. The period after injury is divided into the immediate, acute and chronic stages. In each stage it is shown that pain has only a weak connection to injury but a strong connection to the body state."}
{"id": "PMID:460934", "title": "Responses of spinal cord dorsal horn neurones to non-noxious and noxious cutaneous temperature changes in the spinal rat.", "content": "(1) Lumbar dorsal horn units characterized by their mechanical cutaneous sensitivities were tested for their responses to temperature changes of the skin in the decerebrate spinal rats. (a) Class 1 units (i.e. driven by non-noxious mechanical stimuli) were rarely thermally sensitive. (b) Nearly all class 2 units (i.e. driven by both non-noxious and noxious mechanical stimuli) and 4 of the 5 class 3 units (i.e. driven by noxious mechanical stimuli) were sensitive to temperature changes. (2) According to their thermal response threshold and their response range, these units were divided into 3 groups. (a) Warming units whose response threshold and maximum response were below 42.5 degrees C. Such units were rarely encountered. (b) Warming/noxious heat units whose response threshold was below 42.5 degrees C but with a maximum response above this temperature. They represented approximatively one-third of the radiant heat-sensitive units. (c) Noxious heat units whose response threshold was above 42.5 degrees C and maximum discharge several degrees above it. Approximately 50% of units activated by radiant heat belonged to this group. (3) Responses to radiant heat stimulation were frequently affected by a first noxious heat application. It consisted: --in a threshold decrease and/or an increase of their cellular discharge for a same temperature range. Only observed for warming/noxious heat units and noxious heat units, this sensitization phenomenon predminantly affected noxious heat units. --in a decrease of cellular discharge for a same temperature range. This desensitization phenomenon was observed for the 3 groups of units driven by radiant heat but mainly for warming units. (4) Supramaximal transcutaneous electrical stimulation revealed that nearly all the thermally sensitive units received A delta and/or C inputs. These units were largely distributed throughout the dorsal horn (laminae I, IV and V). Ten of the 12 lamina I units responded to noxious thermal stimuli. (5) These data indicate that an increase in skin temperature is coded at the level of the rat spinal cord dorsal horn by both an increase in discharge of low threshold temperature sensitive units and a progressive recruitment of high threshold units.", "contents": "Responses of spinal cord dorsal horn neurones to non-noxious and noxious cutaneous temperature changes in the spinal rat. (1) Lumbar dorsal horn units characterized by their mechanical cutaneous sensitivities were tested for their responses to temperature changes of the skin in the decerebrate spinal rats. (a) Class 1 units (i.e. driven by non-noxious mechanical stimuli) were rarely thermally sensitive. (b) Nearly all class 2 units (i.e. driven by both non-noxious and noxious mechanical stimuli) and 4 of the 5 class 3 units (i.e. driven by noxious mechanical stimuli) were sensitive to temperature changes. (2) According to their thermal response threshold and their response range, these units were divided into 3 groups. (a) Warming units whose response threshold and maximum response were below 42.5 degrees C. Such units were rarely encountered. (b) Warming/noxious heat units whose response threshold was below 42.5 degrees C but with a maximum response above this temperature. They represented approximatively one-third of the radiant heat-sensitive units. (c) Noxious heat units whose response threshold was above 42.5 degrees C and maximum discharge several degrees above it. Approximately 50% of units activated by radiant heat belonged to this group. (3) Responses to radiant heat stimulation were frequently affected by a first noxious heat application. It consisted: --in a threshold decrease and/or an increase of their cellular discharge for a same temperature range. Only observed for warming/noxious heat units and noxious heat units, this sensitization phenomenon predminantly affected noxious heat units. --in a decrease of cellular discharge for a same temperature range. This desensitization phenomenon was observed for the 3 groups of units driven by radiant heat but mainly for warming units. (4) Supramaximal transcutaneous electrical stimulation revealed that nearly all the thermally sensitive units received A delta and/or C inputs. These units were largely distributed throughout the dorsal horn (laminae I, IV and V). Ten of the 12 lamina I units responded to noxious thermal stimuli. (5) These data indicate that an increase in skin temperature is coded at the level of the rat spinal cord dorsal horn by both an increase in discharge of low threshold temperature sensitive units and a progressive recruitment of high threshold units."}
{"id": "PMID:460935", "title": "Diffuse noxious inhibitory controls (DNIC). I. Effects on dorsal horn convergent neurones in the rat.", "content": "(1) Sixty-eight convergent dorsal horn neurones have been recorded at the lumbar level in anaesthetized intact rats. All cells received prominent A alpha and C fibre afferents and correspondingly could be activated by high and low threshold stimuli applied to the peripheral excitatory receptive field. (2) The activity of 67/68 of these neurones was powerfully inhibited by noxious stimuli applied to various parts of the body. Since non-noxious stimuli were ineffective in this respect, the term \"diffuse noxious inhibitory controls\" (DNIC) is proposed. (3) DNIC could be evoked by noxious pinch applied to the tail, the contralateral hind paw, the forepaws, the ears and the muzzle; the most effective areas were the tail and muzzle. Noxious heat applied to and transcutaneous electrical stimulation of the tail were extemely effective in eliciting DNIC as was the intraperitoneal injection of bradykinin. (4) DNIC strongly depressed by 60-100% both the C fibre response following suprathreshold transcutaneous electrical stimulation and the responses to noxious radiant heat. (5) The spontaneous activity and the responses to low threshold afferents induced either by A alpha threshold electrical or natural stimulation were also powerfully inhibited. (6) In the majority of cases, long lasting post-effects directly related to the duration of conditioning painful stimulus were observed.", "contents": "Diffuse noxious inhibitory controls (DNIC). I. Effects on dorsal horn convergent neurones in the rat. (1) Sixty-eight convergent dorsal horn neurones have been recorded at the lumbar level in anaesthetized intact rats. All cells received prominent A alpha and C fibre afferents and correspondingly could be activated by high and low threshold stimuli applied to the peripheral excitatory receptive field. (2) The activity of 67/68 of these neurones was powerfully inhibited by noxious stimuli applied to various parts of the body. Since non-noxious stimuli were ineffective in this respect, the term \"diffuse noxious inhibitory controls\" (DNIC) is proposed. (3) DNIC could be evoked by noxious pinch applied to the tail, the contralateral hind paw, the forepaws, the ears and the muzzle; the most effective areas were the tail and muzzle. Noxious heat applied to and transcutaneous electrical stimulation of the tail were extemely effective in eliciting DNIC as was the intraperitoneal injection of bradykinin. (4) DNIC strongly depressed by 60-100% both the C fibre response following suprathreshold transcutaneous electrical stimulation and the responses to noxious radiant heat. (5) The spontaneous activity and the responses to low threshold afferents induced either by A alpha threshold electrical or natural stimulation were also powerfully inhibited. (6) In the majority of cases, long lasting post-effects directly related to the duration of conditioning painful stimulus were observed."}
{"id": "PMID:460936", "title": "Diffuse noxious inhibitory controls (DNIC). II. Lack of effect on non-convergent neurones, supraspinal involvement and theoretical implications.", "content": "(1) Diffuse noxious inhibitory controls (DNIC) were tested for their effect on noxious only, non-noxious and proprioceptive cells in the dorsal horn of the intact anaesthetized rat. Unlike convergent neurones, as described in the previous paper, there was no effect of DNIC on these neurones. It is concluded that convergent neurones are specifically inhibited by DNIC. (2) The effect of DNIC could not be demonstrated for convergent neurones in the spinal animal. Thus the neuronal substrate for DNIC must involve supraspinal structures. (3) Because of the level of firing in convergent neurones induced by hair and touch receptors, presumably constantly and randomly activated in the freely moving animal, a noxious message arriving at higher centres may be partly masked by this background noise. On the basis of the known role of convergent neurones in nociception, we propose the following mechanism which may interpret this paradoxical convergence: two pools of convergent neurones are influenced by a painful peripheral stimulation, one segmental pool being activated whilst the remaining population of cells is inhibited; the \"contrast\" between the messages from these two pools may well produce a significant pain signalling output from the convergent dorsal horn cells. (4) These results and their theoretical implications are discussed with regard to the concept of the \"analgesic system\", certain clinical observations and the paradoxical pain relieving effects of counterirritation and some forms of acupuncture.", "contents": "Diffuse noxious inhibitory controls (DNIC). II. Lack of effect on non-convergent neurones, supraspinal involvement and theoretical implications. (1) Diffuse noxious inhibitory controls (DNIC) were tested for their effect on noxious only, non-noxious and proprioceptive cells in the dorsal horn of the intact anaesthetized rat. Unlike convergent neurones, as described in the previous paper, there was no effect of DNIC on these neurones. It is concluded that convergent neurones are specifically inhibited by DNIC. (2) The effect of DNIC could not be demonstrated for convergent neurones in the spinal animal. Thus the neuronal substrate for DNIC must involve supraspinal structures. (3) Because of the level of firing in convergent neurones induced by hair and touch receptors, presumably constantly and randomly activated in the freely moving animal, a noxious message arriving at higher centres may be partly masked by this background noise. On the basis of the known role of convergent neurones in nociception, we propose the following mechanism which may interpret this paradoxical convergence: two pools of convergent neurones are influenced by a painful peripheral stimulation, one segmental pool being activated whilst the remaining population of cells is inhibited; the \"contrast\" between the messages from these two pools may well produce a significant pain signalling output from the convergent dorsal horn cells. (4) These results and their theoretical implications are discussed with regard to the concept of the \"analgesic system\", certain clinical observations and the paradoxical pain relieving effects of counterirritation and some forms of acupuncture."}
{"id": "PMID:460937", "title": "Brain evoked potentials are functional correlates of induced pain in man.", "content": "Electrical potentials evoked by 5 intensities of painful dental stimulation were recorded at the scalp. During testing, volunteers indicated subjective painfulness by verbal pain ratings and visual analogue scales. Evoked potentials (EPs) to each intensity, observed between 50 and 400 msec, were characterized by 4 waveform components. The peak-to-peak amplitudes, but not the peak latencies, of all 4 EP components systematically increased with increased stimulation. The amplitudes of the two earlier components correlated with stimulus intensity when the effect of subjective painfulness was controlled, but this was not the case for the later components. In contrast, the amplitudes of the two later components were associated with subjective painfulness but not with stimulus intensity. A strong linear relationship was observed between subjective painfulness and peak-to-peak amplitude for the EP component observed between 175 and 260 msec. The data suggest that the earlier EP components may reflect sensory transmission processes while the later components indicate brain activity when pain is perceived.", "contents": "Brain evoked potentials are functional correlates of induced pain in man. Electrical potentials evoked by 5 intensities of painful dental stimulation were recorded at the scalp. During testing, volunteers indicated subjective painfulness by verbal pain ratings and visual analogue scales. Evoked potentials (EPs) to each intensity, observed between 50 and 400 msec, were characterized by 4 waveform components. The peak-to-peak amplitudes, but not the peak latencies, of all 4 EP components systematically increased with increased stimulation. The amplitudes of the two earlier components correlated with stimulus intensity when the effect of subjective painfulness was controlled, but this was not the case for the later components. In contrast, the amplitudes of the two later components were associated with subjective painfulness but not with stimulus intensity. A strong linear relationship was observed between subjective painfulness and peak-to-peak amplitude for the EP component observed between 175 and 260 msec. The data suggest that the earlier EP components may reflect sensory transmission processes while the later components indicate brain activity when pain is perceived."}
{"id": "PMID:460938", "title": "The submaximal effort tourniquet test: its use in evaluating experimental and chronic pain.", "content": "The submaximal effort tourniquet test has been widely used to evaluate experimental and chronic pain; however, there has been great variation in the manner in which the test has been applied. The present study systemitically evaluates how different levels of exercise duration and effort affect subjects' report of pain. The data indicate the following: (1) the manner in which the exercise is performed has an important effect on subjects' rating of pain, and (2) under all conditions studied, the pain ratings do not increase as a linear function of time. Both findings warrant precautions when using the submaximal effort tourniquet test to study experimental and chronic pain.", "contents": "The submaximal effort tourniquet test: its use in evaluating experimental and chronic pain. The submaximal effort tourniquet test has been widely used to evaluate experimental and chronic pain; however, there has been great variation in the manner in which the test has been applied. The present study systemitically evaluates how different levels of exercise duration and effort affect subjects' report of pain. The data indicate the following: (1) the manner in which the exercise is performed has an important effect on subjects' rating of pain, and (2) under all conditions studied, the pain ratings do not increase as a linear function of time. Both findings warrant precautions when using the submaximal effort tourniquet test to study experimental and chronic pain."}
{"id": "PMID:460940", "title": "An improved procedure for accurate assays of factor VIII.", "content": "An improved one-stage method for accurate assays of factor VIII combines highly reproducible end points with elimination of temporal drift and of subjective factors involved in graphic analysis. Activated deficient plasma substrate (ADPS) is used as a single reagent for parallel tests. The assays can be performed manually or, with much greater precision, on an 8-channel coagulation meter of new design in which end points are recorded automatically and depend on an abrupt clearing of agitated cloudy suspensions. The coagulation time readings are reproducible to 0.5%. Factor VIII levels are read off a standard table or computed from a general equation which is readily programmable on a pocket calculator and is valid over a wide range of concentrations, including very low plasma levels.", "contents": "An improved procedure for accurate assays of factor VIII. An improved one-stage method for accurate assays of factor VIII combines highly reproducible end points with elimination of temporal drift and of subjective factors involved in graphic analysis. Activated deficient plasma substrate (ADPS) is used as a single reagent for parallel tests. The assays can be performed manually or, with much greater precision, on an 8-channel coagulation meter of new design in which end points are recorded automatically and depend on an abrupt clearing of agitated cloudy suspensions. The coagulation time readings are reproducible to 0.5%. Factor VIII levels are read off a standard table or computed from a general equation which is readily programmable on a pocket calculator and is valid over a wide range of concentrations, including very low plasma levels."}
{"id": "PMID:460941", "title": "Red cell stroma, a stable standard for assessment of platelet procoagulant release.", "content": "Platelet function in vitro may be investigated in various ways. One of the accepted methods involves examination of platelet procoagulant release. Optimal conditions relating to maximal release have not been defined. Red cell stroma phospholipid can substitute for the platelet procoagulant in many in vitro tests (Quick et al., 1954, 1957; Margolis, 1961; Lovric & Margolis, 1964). Using a multiple channel coagulation meter, an assay system was developed for testing both red cell stroma and platelet procoagulants. Maximum procoagulant release was obtained by lysing in alkaline hypotonic solutions or by sonication. The system was tested in a one-stage kaolin-activated platelet-free plasma substrate, obtained from fresh pooled citrated plasma. With this method the amount of available procoagulant could be related to the numbers of red blood cells or platelets. The results indicated that one platelet provided procoagulant equivalent to that derived from 0.7 red cells. Procoagulant release was studied under various conditions and expressed in terms of stable (red cell) standard. Platelets subjected to hypotonic stress or factor 3 release (Hardisty test) were studied quantitatively. The addition of sulphinpyrazone (Anturan) had no observable effect on platelet release in this system.", "contents": "Red cell stroma, a stable standard for assessment of platelet procoagulant release. Platelet function in vitro may be investigated in various ways. One of the accepted methods involves examination of platelet procoagulant release. Optimal conditions relating to maximal release have not been defined. Red cell stroma phospholipid can substitute for the platelet procoagulant in many in vitro tests (Quick et al., 1954, 1957; Margolis, 1961; Lovric & Margolis, 1964). Using a multiple channel coagulation meter, an assay system was developed for testing both red cell stroma and platelet procoagulants. Maximum procoagulant release was obtained by lysing in alkaline hypotonic solutions or by sonication. The system was tested in a one-stage kaolin-activated platelet-free plasma substrate, obtained from fresh pooled citrated plasma. With this method the amount of available procoagulant could be related to the numbers of red blood cells or platelets. The results indicated that one platelet provided procoagulant equivalent to that derived from 0.7 red cells. Procoagulant release was studied under various conditions and expressed in terms of stable (red cell) standard. Platelets subjected to hypotonic stress or factor 3 release (Hardisty test) were studied quantitatively. The addition of sulphinpyrazone (Anturan) had no observable effect on platelet release in this system."}
{"id": "PMID:460942", "title": "A rapid method for the quantitation of haemoglobin A2.", "content": "A rapid accurate and inexpensive technique for the measurement of haemoglobin A2 has been devised. It uses a small plastic device which embodies a miniature chromatographic column and reservoirs for the eluting buffer and eluates. The device is charged with whole blood, pH 8.25 buffer and centrifuged. The eluate is removed and the centrifugation repeated using pH 7.0 buffer to remove the remaining haemoglobin. The haemoglobin concentration in the 2 volumes of eluates is measured in a spectrophotometer at 414 nm and the percentage of HbA2 calculated. Results are similar to those obtained by standard methods.", "contents": "A rapid method for the quantitation of haemoglobin A2. A rapid accurate and inexpensive technique for the measurement of haemoglobin A2 has been devised. It uses a small plastic device which embodies a miniature chromatographic column and reservoirs for the eluting buffer and eluates. The device is charged with whole blood, pH 8.25 buffer and centrifuged. The eluate is removed and the centrifugation repeated using pH 7.0 buffer to remove the remaining haemoglobin. The haemoglobin concentration in the 2 volumes of eluates is measured in a spectrophotometer at 414 nm and the percentage of HbA2 calculated. Results are similar to those obtained by standard methods."}
{"id": "PMID:460943", "title": "The quantitation of haemoglobin A2 and haemoglobin F: a report of the techniques review group of the Thalassaemia Society of Victoria.", "content": "A techniques review group was established in 1975 to investigate the reproducibility of tests for HbA2 and HbF for the diagnosis of beta thalassaemia. Over a 3-year period, 22 samples were distributed for analysis by up to 14 laboratories. Of 250 estimations of HbA2, 26 (10.4%) were at variance with the majority result. The largest number of variant results were obtained in samples with a HbA2 close to the upper limit of normal. Of 238 HbF determinations, 29 (12%) were equivocal or at variance with the majority result. It is concluded that the techniques of HbA2 and HbF estimation as currently practised by several laboratories are not sufficiently accurate, and that, therefore, a number of cases of beta thalassaemia are not being diagnosed. It is the opinion of those participating in the study that the survey is of value in providing a continuing review of techniques and a forum for discussion.", "contents": "The quantitation of haemoglobin A2 and haemoglobin F: a report of the techniques review group of the Thalassaemia Society of Victoria. A techniques review group was established in 1975 to investigate the reproducibility of tests for HbA2 and HbF for the diagnosis of beta thalassaemia. Over a 3-year period, 22 samples were distributed for analysis by up to 14 laboratories. Of 250 estimations of HbA2, 26 (10.4%) were at variance with the majority result. The largest number of variant results were obtained in samples with a HbA2 close to the upper limit of normal. Of 238 HbF determinations, 29 (12%) were equivocal or at variance with the majority result. It is concluded that the techniques of HbA2 and HbF estimation as currently practised by several laboratories are not sufficiently accurate, and that, therefore, a number of cases of beta thalassaemia are not being diagnosed. It is the opinion of those participating in the study that the survey is of value in providing a continuing review of techniques and a forum for discussion."}
{"id": "PMID:460944", "title": "Oestrogen receptor protein in breast cancer: a local study.", "content": "An assay using charcoal adsorption to separate bound oestradiol from free oestradiol has been set up to study the oestrogen receptor (ER) status of patients with all stages of breast carcinoma. The specificity of the oestradiol uptake was checked by competitive pre-incubation with anti-oestrogens and non radioactive oestradiol. Eighty-three of 137 (61%) primary breast lesions and 42 of 103 (41%) metastatic lesions studied had a (positive) oestradiol binding capacity. Such results are congruous in respect of age groups and receptor status with other published data.", "contents": "Oestrogen receptor protein in breast cancer: a local study. An assay using charcoal adsorption to separate bound oestradiol from free oestradiol has been set up to study the oestrogen receptor (ER) status of patients with all stages of breast carcinoma. The specificity of the oestradiol uptake was checked by competitive pre-incubation with anti-oestrogens and non radioactive oestradiol. Eighty-three of 137 (61%) primary breast lesions and 42 of 103 (41%) metastatic lesions studied had a (positive) oestradiol binding capacity. Such results are congruous in respect of age groups and receptor status with other published data."}
{"id": "PMID:460945", "title": "Human malignant melanoma cell lines.", "content": "A series of 10 cell lines established from human malignant melanomas is described. The morphology varied but, in general, was epithelioid. Five produced visible melanin in culture but others produced melanin precursors. All lines were aneuploid and each had a distinctive human karyotype. One line appeared not to have the same sex as the patient but its karyotype was distinct from that of other lines.", "contents": "Human malignant melanoma cell lines. A series of 10 cell lines established from human malignant melanomas is described. The morphology varied but, in general, was epithelioid. Five produced visible melanin in culture but others produced melanin precursors. All lines were aneuploid and each had a distinctive human karyotype. One line appeared not to have the same sex as the patient but its karyotype was distinct from that of other lines."}
{"id": "PMID:460946", "title": "A systematic study of wound contraction in mammalian skin.", "content": "The phenomenon of wound contraction has been studied in the skin of rats, rabbits and guinea pigs with the aim of obtaining a standardized wound system to be used for biological assay of in vivo agonists and antagonists of granulation tissue contraction. The sex of the animal, the time of day of wounding, the size and the shape of the wounds all had no apparent influence on the wound contraction curves expressed as per cent of original area against time. The test animal's age and species did have a significant influence on contraction of skin wounds which by analogy with results obtained with rigid splints attached to the skin could be attributed to variations in the thickness, rigidity and fixation of the skin to deep tissues. The shape of the final scar was influenced by the position of the wound on the body surface. The major factor in this instance was considered to be tensile forces acting within the skin. Wound contraction curves in rats and rabbits showed three distinct phases, namely early closure, stationary and logarithmic closure. Guinea pigs lacked the early closure phase. The exact role of the panniculus carnosus in wound healing is considered to be worthy of detailed study.", "contents": "A systematic study of wound contraction in mammalian skin. The phenomenon of wound contraction has been studied in the skin of rats, rabbits and guinea pigs with the aim of obtaining a standardized wound system to be used for biological assay of in vivo agonists and antagonists of granulation tissue contraction. The sex of the animal, the time of day of wounding, the size and the shape of the wounds all had no apparent influence on the wound contraction curves expressed as per cent of original area against time. The test animal's age and species did have a significant influence on contraction of skin wounds which by analogy with results obtained with rigid splints attached to the skin could be attributed to variations in the thickness, rigidity and fixation of the skin to deep tissues. The shape of the final scar was influenced by the position of the wound on the body surface. The major factor in this instance was considered to be tensile forces acting within the skin. Wound contraction curves in rats and rabbits showed three distinct phases, namely early closure, stationary and logarithmic closure. Guinea pigs lacked the early closure phase. The exact role of the panniculus carnosus in wound healing is considered to be worthy of detailed study."}
{"id": "PMID:460947", "title": "The pathology of occipital encephalocoele and a discussion of the pathogenesis.", "content": "The midline structures of 5 infant brains with occipital encephalocoeles were studied. Though all cases showed a uniform finding of a midline defect of the occipital bone with a herniated mass, the extent of the bony defect, the amount and nature of the herniated tissues and the degree of distortion of the intracranial structures varied markedly. Two of the cases showed multiple associated systemic malformations and another case had an inverse cerebellum. In spite of such a marked variability in presentation, the common findings of herniation and displacement of the mesencephalon and roof of the diencephalon allowed us to postulate that the primary defect of occipital encephalocoeles is an abnormality of the tissues overlying the mesencephalon of the developing brain. Initial herniation of the mesencephalon and subsequent movement of the rest of the brain as a result of growth determines the tissues present in the hernia sac at birth. Other theories of genesis of occipital encephalocoeles are discussed.", "contents": "The pathology of occipital encephalocoele and a discussion of the pathogenesis. The midline structures of 5 infant brains with occipital encephalocoeles were studied. Though all cases showed a uniform finding of a midline defect of the occipital bone with a herniated mass, the extent of the bony defect, the amount and nature of the herniated tissues and the degree of distortion of the intracranial structures varied markedly. Two of the cases showed multiple associated systemic malformations and another case had an inverse cerebellum. In spite of such a marked variability in presentation, the common findings of herniation and displacement of the mesencephalon and roof of the diencephalon allowed us to postulate that the primary defect of occipital encephalocoeles is an abnormality of the tissues overlying the mesencephalon of the developing brain. Initial herniation of the mesencephalon and subsequent movement of the rest of the brain as a result of growth determines the tissues present in the hernia sac at birth. Other theories of genesis of occipital encephalocoeles are discussed."}
{"id": "PMID:460948", "title": "Histology of normal bone--a computerized study in the iliac crest.", "content": "The application of an electronic digitizer and a computerized program for the quantitative assessment of bone morphology is reported. The method was used to measure bone surfaces and areas in undecalcified sections of iliac crest in 50 control subjects at necropsy.", "contents": "Histology of normal bone--a computerized study in the iliac crest. The application of an electronic digitizer and a computerized program for the quantitative assessment of bone morphology is reported. The method was used to measure bone surfaces and areas in undecalcified sections of iliac crest in 50 control subjects at necropsy."}
{"id": "PMID:460949", "title": "Marchiafava-Bignami disease in a non-alcoholic Indian male.", "content": "Marchiafava-Bignami disease, a rare affliction of alcoholic males, is described in a severely malnourished Malaysian Indian male who took no alcohol. It is the second report of the disease in an Asian and represents one of the few cases which have occurred in non-alcoholics. Besides the pathognomonic demyelination of the central portion of the corpus callosum, there were striking demyelinative plaques in the subcortical white matter. In addition, neuropathological features of Wernicke's disease were found suggesting that severe malnutrition with thiamine deficiency was probably the cause of his demise.", "contents": "Marchiafava-Bignami disease in a non-alcoholic Indian male. Marchiafava-Bignami disease, a rare affliction of alcoholic males, is described in a severely malnourished Malaysian Indian male who took no alcohol. It is the second report of the disease in an Asian and represents one of the few cases which have occurred in non-alcoholics. Besides the pathognomonic demyelination of the central portion of the corpus callosum, there were striking demyelinative plaques in the subcortical white matter. In addition, neuropathological features of Wernicke's disease were found suggesting that severe malnutrition with thiamine deficiency was probably the cause of his demise."}
{"id": "PMID:460950", "title": "The pathological assessment of ovarian neoplasms. II: The proliferating 'epithelial' tumours.", "content": "All neoplasms of the ovary encountered in a 25-year study period at the King George V Memorial Hospital were classified according to the World Health Organisation Histological Classification of Ovarian Tumours. Of these, one thousand fell into the category designated as 'common epithelial tumours'. In this report a detailed histological assessment of the 144 proliferating epithelial tumours is presented, including the criteria for distinguishing them from their benign and malignant counterparts and for histological typing and grading of these tumours.", "contents": "The pathological assessment of ovarian neoplasms. II: The proliferating 'epithelial' tumours. All neoplasms of the ovary encountered in a 25-year study period at the King George V Memorial Hospital were classified according to the World Health Organisation Histological Classification of Ovarian Tumours. Of these, one thousand fell into the category designated as 'common epithelial tumours'. In this report a detailed histological assessment of the 144 proliferating epithelial tumours is presented, including the criteria for distinguishing them from their benign and malignant counterparts and for histological typing and grading of these tumours."}
{"id": "PMID:460951", "title": "Atypical mycobacteria causing non-pulmonary disease in Queensland.", "content": "During the period 1971--7, the Tuberculosis Reference Laboratory in Queensland dealt with 52 isolates of atypical mycobacteria made from non-pulmonary sites under circumstances suggesting complicity in disease. Twenty-four isolates belonging to the MAIS complex were associated with lymph node infections in children. Twelve isolates belonged to the M. fortuitum-chelonei complex; most were from superficial abscesses. Five cases of M. marinum infection and 8 of M. ulcerans disease were detected.", "contents": "Atypical mycobacteria causing non-pulmonary disease in Queensland. During the period 1971--7, the Tuberculosis Reference Laboratory in Queensland dealt with 52 isolates of atypical mycobacteria made from non-pulmonary sites under circumstances suggesting complicity in disease. Twenty-four isolates belonging to the MAIS complex were associated with lymph node infections in children. Twelve isolates belonged to the M. fortuitum-chelonei complex; most were from superficial abscesses. Five cases of M. marinum infection and 8 of M. ulcerans disease were detected."}
{"id": "PMID:460952", "title": "Chronic asymptomatic genital tract infection and semen quality.", "content": "Chronic asymptomatic genital tract infection has been postulated to cause infertility in man. This study demonstrates the correlation of positive seminal fluid bacteriological cultures with abnormal parameters of seminal fluid analysis in the male partners of 100 uninvestigated couples presenting with infertility. Gram negative bacteria were cultured only in patients with semen abnormalities while Gram positive bacteria were cultured from both normal and abnormal semen specimens.", "contents": "Chronic asymptomatic genital tract infection and semen quality. Chronic asymptomatic genital tract infection has been postulated to cause infertility in man. This study demonstrates the correlation of positive seminal fluid bacteriological cultures with abnormal parameters of seminal fluid analysis in the male partners of 100 uninvestigated couples presenting with infertility. Gram negative bacteria were cultured only in patients with semen abnormalities while Gram positive bacteria were cultured from both normal and abnormal semen specimens."}
{"id": "PMID:460953", "title": "An improved screening technique for isolation of Pseudomonas pseudomallei from clinical specimens.", "content": "A selective medium consisting of trypticase soy agar with 4% glycerol, 5 mg/l crystal violet, 50 mg/l neutral red and 4 mg/l of gentamicin was devised for isolation of Pseudomonas pseudomallei from clinical specimens. Absorption of neutral red was found to be suitable for differentiating this organism from other bacteria, while gentamicin was effective in selecting Ps. pseudomallei from organisms commonly found in clinical material. The medium was more suitable for screening clinical specimens than MacConkey's agar with colistin-S because it was more selective and allowed multiple specimens to be inoculated on a single plate. Eight thousand clinical specimens from an area endemic for melioidosis were screened on the selective medium. This resulted in the recovery of 8 isolates of Ps. pseudomallei that would not have been detected using routine culture media alone.", "contents": "An improved screening technique for isolation of Pseudomonas pseudomallei from clinical specimens. A selective medium consisting of trypticase soy agar with 4% glycerol, 5 mg/l crystal violet, 50 mg/l neutral red and 4 mg/l of gentamicin was devised for isolation of Pseudomonas pseudomallei from clinical specimens. Absorption of neutral red was found to be suitable for differentiating this organism from other bacteria, while gentamicin was effective in selecting Ps. pseudomallei from organisms commonly found in clinical material. The medium was more suitable for screening clinical specimens than MacConkey's agar with colistin-S because it was more selective and allowed multiple specimens to be inoculated on a single plate. Eight thousand clinical specimens from an area endemic for melioidosis were screened on the selective medium. This resulted in the recovery of 8 isolates of Ps. pseudomallei that would not have been detected using routine culture media alone."}
{"id": "PMID:460984", "title": "Hypoglycemia in children.", "content": "The factors that sustain postabsorptive glucose concentrations have been analyzed and the adverse effects of various hypoglycemic disorders on these factors examined. The role of alanine has been reviewed and the importance of glycerol as a precursor of glucose and of ketones as a fuel substitute for glucose emphasized. Finally, we have suggested that fasting functional hypoglycemia replace ketotic hypoglycemia as a descriptive term and that we relinquish the concept of leucine-sensitive hypoglycemia as a specific entity.", "contents": "Hypoglycemia in children. The factors that sustain postabsorptive glucose concentrations have been analyzed and the adverse effects of various hypoglycemic disorders on these factors examined. The role of alanine has been reviewed and the importance of glycerol as a precursor of glucose and of ketones as a fuel substitute for glucose emphasized. Finally, we have suggested that fasting functional hypoglycemia replace ketotic hypoglycemia as a descriptive term and that we relinquish the concept of leucine-sensitive hypoglycemia as a specific entity."}
{"id": "PMID:461032", "title": "Depth adjacency and induced motion.", "content": "Induced motion was investigated as a function of the stereoscopic separation of the test and inducing object and the instructions to attend to or to ignore the inducing object. It was found that stereoscopically displacing the test object from the inducing object with both kinds of instructions resulted in a decrease in the magnitude of induction particularly with crossed disparity. These results are consistent with the adjacency principle and with the ability of attention as well as adjacency to modify the magnitude of the induced motion.", "contents": "Depth adjacency and induced motion. Induced motion was investigated as a function of the stereoscopic separation of the test and inducing object and the instructions to attend to or to ignore the inducing object. It was found that stereoscopically displacing the test object from the inducing object with both kinds of instructions resulted in a decrease in the magnitude of induction particularly with crossed disparity. These results are consistent with the adjacency principle and with the ability of attention as well as adjacency to modify the magnitude of the induced motion."}
{"id": "PMID:461033", "title": "Perceptual reactance and task performance.", "content": "57 female subjects participated in a study in which perceptual reactance was assessed by the kinesthetic figural aftereffects test. Subjects identified as augmenters were quicker than reducers on a disjunctive reaction time task and a significant interactive effect was found on a paired-associate task. Augmenters performed better on a similar list while reducers were more effective on a dissimilar list.", "contents": "Perceptual reactance and task performance. 57 female subjects participated in a study in which perceptual reactance was assessed by the kinesthetic figural aftereffects test. Subjects identified as augmenters were quicker than reducers on a disjunctive reaction time task and a significant interactive effect was found on a paired-associate task. Augmenters performed better on a similar list while reducers were more effective on a dissimilar list."}
{"id": "PMID:461034", "title": "Report on blind subjects' tactile and auditory recognition for environmental stimuli.", "content": "In two related experiments on recognition-on touch and audition-accuracy rates were obtained from congenitally blind subjects and compared with those for normally sighted subjects. In Exp. 1, 5 blind subjects inspected, i.e., handled, 150 common objects and were tested after a delay of 7 days. In Exp. 2, 9 blind subjects listened to 194 naturalistic sounds and were  also tested after a 7-day delay. Accuracy of tactile recognition for the blind was 89.4% while it was 87.9% for the normally sighted. Sound recognition by blind subjects was 76.6% and for the normally sighted it was 78.4%. Neither difference was statistically significant.", "contents": "Report on blind subjects' tactile and auditory recognition for environmental stimuli. In two related experiments on recognition-on touch and audition-accuracy rates were obtained from congenitally blind subjects and compared with those for normally sighted subjects. In Exp. 1, 5 blind subjects inspected, i.e., handled, 150 common objects and were tested after a delay of 7 days. In Exp. 2, 9 blind subjects listened to 194 naturalistic sounds and were  also tested after a 7-day delay. Accuracy of tactile recognition for the blind was 89.4% while it was 87.9% for the normally sighted. Sound recognition by blind subjects was 76.6% and for the normally sighted it was 78.4%. Neither difference was statistically significant."}
{"id": "PMID:461035", "title": "A diet and exercise regimen: its effect upon mental acuity and personality, a pilot study.", "content": "Of the 75 patients in the February 1977 \"class\" at the Longevity Research Institute, Santa Barbara, California, 11 volunteers were pre- and posttested (21- to 23-day intervals) with the MMPI, 13 with the California Psychological Inventory, and 17 with four subtests of the WAIS (total: 21 males, 10 females). Ten of the (total) 32 scales showed changes in the predicted, favorable direction, statistically significant at the .05 level or better by t test. Further the number of scales changing in the predicted, desirable direction for each of the three tests was statistically significant at the .06, and .001, and .06 levels (sign test), respectively. The pilot results indicate improvement in psychological factors and mental acuity within a month's time concomitant with the diet, activity, and educational program and warrant further investigation.", "contents": "A diet and exercise regimen: its effect upon mental acuity and personality, a pilot study. Of the 75 patients in the February 1977 \"class\" at the Longevity Research Institute, Santa Barbara, California, 11 volunteers were pre- and posttested (21- to 23-day intervals) with the MMPI, 13 with the California Psychological Inventory, and 17 with four subtests of the WAIS (total: 21 males, 10 females). Ten of the (total) 32 scales showed changes in the predicted, favorable direction, statistically significant at the .05 level or better by t test. Further the number of scales changing in the predicted, desirable direction for each of the three tests was statistically significant at the .06, and .001, and .06 levels (sign test), respectively. The pilot results indicate improvement in psychological factors and mental acuity within a month's time concomitant with the diet, activity, and educational program and warrant further investigation."}
{"id": "PMID:461036", "title": "Skill performance on biorhythm theory's physically critical day.", "content": "The hypothesis of a critical day in biorhythm theory predicts that skill performance would be lower than normal on a physically critical day. Participants in an archery league were observed in 346 events. Indoor archery performance provided scores identical to the goal of the task and unaffected by environmental conditions or other competitors. Above-average performances on 34 physically critical days did not differ significantly from the population proportion of above-average performances. No support for the hypothesis was provided by this observation of archery performance.", "contents": "Skill performance on biorhythm theory's physically critical day. The hypothesis of a critical day in biorhythm theory predicts that skill performance would be lower than normal on a physically critical day. Participants in an archery league were observed in 346 events. Indoor archery performance provided scores identical to the goal of the task and unaffected by environmental conditions or other competitors. Above-average performances on 34 physically critical days did not differ significantly from the population proportion of above-average performances. No support for the hypothesis was provided by this observation of archery performance."}
{"id": "PMID:461037", "title": "On the validity of hypotheses derived from the Rorschach: V. The relationship between form level and ego strength.", "content": "Although Rorschach interpreted from level as a perceptual and conceptual aspect of intelligence, Beck and the more current meaning assigned to form level relates these functions to ego strength. F+%, therefore, has come to be regarded as a measure of ego strength. The research examining the validity of this assumption was reviewed and Beck's hypothesis was seen to be supported. The research supports the clinical use of F+% as a measure of ego strength, but further research might help clarify what actual psychological processes are involved in the creation of responses of good form.", "contents": "On the validity of hypotheses derived from the Rorschach: V. The relationship between form level and ego strength. Although Rorschach interpreted from level as a perceptual and conceptual aspect of intelligence, Beck and the more current meaning assigned to form level relates these functions to ego strength. F+%, therefore, has come to be regarded as a measure of ego strength. The research examining the validity of this assumption was reviewed and Beck's hypothesis was seen to be supported. The research supports the clinical use of F+% as a measure of ego strength, but further research might help clarify what actual psychological processes are involved in the creation of responses of good form."}
{"id": "PMID:461038", "title": "Handedness of handedness researchers.", "content": "This study tested the hypothesis that laterality and handedness researchers are more likely than other researchers to be left-handed. A questionnaire assessing hand preference was sent to authors of articles which either dealt with handedness (N = 50) or other aspects of laterality (N = 100) or did not concern laterality (N = 50). The proportion of left-handers was highest in the handedness group (12.9%) and lowest in the non-laterality group (5.0%), but this difference did not reach significance.", "contents": "Handedness of handedness researchers. This study tested the hypothesis that laterality and handedness researchers are more likely than other researchers to be left-handed. A questionnaire assessing hand preference was sent to authors of articles which either dealt with handedness (N = 50) or other aspects of laterality (N = 100) or did not concern laterality (N = 50). The proportion of left-handers was highest in the handedness group (12.9%) and lowest in the non-laterality group (5.0%), but this difference did not reach significance."}
{"id": "PMID:461039", "title": "Influence of functional electrical stimulation training on premotor and motor reaction time.", "content": "This study was designed to investigate the influence of functional electrical stimulation on the improvement of reaction time and its fractionated components during learning an horizontal arm-sweep task. 62 subjects participated and were divided into control, stimulation, and trail/stimulation groups. Functional electrical stimulation was an effective method of improving total reaction time and its central component, premotor time.", "contents": "Influence of functional electrical stimulation training on premotor and motor reaction time. This study was designed to investigate the influence of functional electrical stimulation on the improvement of reaction time and its fractionated components during learning an horizontal arm-sweep task. 62 subjects participated and were divided into control, stimulation, and trail/stimulation groups. Functional electrical stimulation was an effective method of improving total reaction time and its central component, premotor time."}
{"id": "PMID:461040", "title": "Pupillary attributions of college students to happy and angry faces.", "content": "As evidence for an hypothesis that pupil size plays an important role in nonverbal communication, Hess (1975) has reported that adults draw in appropriately sized pupils on his happy and angry faces task. However, he did not report a statistical test of his data. In this study, we replicated Hess' research and found congruent with his hypothesis that college students (n = 223) draw in significantly larger pupils on the happy face.", "contents": "Pupillary attributions of college students to happy and angry faces. As evidence for an hypothesis that pupil size plays an important role in nonverbal communication, Hess (1975) has reported that adults draw in appropriately sized pupils on his happy and angry faces task. However, he did not report a statistical test of his data. In this study, we replicated Hess' research and found congruent with his hypothesis that college students (n = 223) draw in significantly larger pupils on the happy face."}
{"id": "PMID:461041", "title": "Electrodermal measures of attention and effort to stimulus onset and offset for intramodal and intermodal tasks.", "content": "Two experiments were designed to assess the relationship between task difficulty and arousal. Electrodermal measures of tonic and phasic arousal to four levels of task difficulty at stimulus onset and offset were studied in college students for intramodal and intermodal tasks. The students were presented for 18 trails with visual-auditory or visual-visual stimuli with either 2.0-, 0.2-, or 0.02-sec difference between stimuli onset or offset, and asked to judge which stimulus came on or went off first or merely to observe the stimuli. Both frequency and amplitude of skin conductance responses reliably differentiated the levels of task difficulty for both the intramodal and the intermodal task. None of the measures of tonic level of arousal was reliable. Electrodermal measures of phasic responses accurately reflected the task demands.", "contents": "Electrodermal measures of attention and effort to stimulus onset and offset for intramodal and intermodal tasks. Two experiments were designed to assess the relationship between task difficulty and arousal. Electrodermal measures of tonic and phasic arousal to four levels of task difficulty at stimulus onset and offset were studied in college students for intramodal and intermodal tasks. The students were presented for 18 trails with visual-auditory or visual-visual stimuli with either 2.0-, 0.2-, or 0.02-sec difference between stimuli onset or offset, and asked to judge which stimulus came on or went off first or merely to observe the stimuli. Both frequency and amplitude of skin conductance responses reliably differentiated the levels of task difficulty for both the intramodal and the intermodal task. None of the measures of tonic level of arousal was reliable. Electrodermal measures of phasic responses accurately reflected the task demands."}
{"id": "PMID:461042", "title": "Effect of varying picture-sentence congruence on children's free recall.", "content": "70 fourth-grade children were shown objects arbitrarily arranged in an integrated scene. Subjects were randomly assigned to conditions which either presented a sentence that correctly labeled and correctly described the physical arrangement of the objects, presented a sentence containing the correct labels of the objects but not the correct physical arrangement, or presented a sentence which did not contain the correct labels and incorrectly described the physical arrangement. Control conditions either provided subjects with correct labels or omitted presentation of verbal prompts. Congruence between the object display and the sentence produced significantly higher recall and clustering than the incongruence or control conditions. The incongruence conditions did not produce significantly higher recall than the control conditions, suggesting that incongruence interferes with formation of stable grouping of items which appears to be an important factor in facilitating free recall.", "contents": "Effect of varying picture-sentence congruence on children's free recall. 70 fourth-grade children were shown objects arbitrarily arranged in an integrated scene. Subjects were randomly assigned to conditions which either presented a sentence that correctly labeled and correctly described the physical arrangement of the objects, presented a sentence containing the correct labels of the objects but not the correct physical arrangement, or presented a sentence which did not contain the correct labels and incorrectly described the physical arrangement. Control conditions either provided subjects with correct labels or omitted presentation of verbal prompts. Congruence between the object display and the sentence produced significantly higher recall and clustering than the incongruence or control conditions. The incongruence conditions did not produce significantly higher recall than the control conditions, suggesting that incongruence interferes with formation of stable grouping of items which appears to be an important factor in facilitating free recall."}
{"id": "PMID:461044", "title": "Practice effects within a multiple-phase Bender administration.", "content": "Practice effects within a multiple-phase administration of the Bender Visual-motor Gestalt Test were investigated. The addition of a 5-sec. Flash phase prior to the standard presentation did not significantly influence performance on the standard presentation. Students who attempted both the Flash and standard administration prior to a recall phase remembered significantly more designs than students receiving only the standard administration prior to recall. Those skills possibly measured by the multiple phase Bender format are discussed and the need for research using the three-phase method is suggested.", "contents": "Practice effects within a multiple-phase Bender administration. Practice effects within a multiple-phase administration of the Bender Visual-motor Gestalt Test were investigated. The addition of a 5-sec. Flash phase prior to the standard presentation did not significantly influence performance on the standard presentation. Students who attempted both the Flash and standard administration prior to a recall phase remembered significantly more designs than students receiving only the standard administration prior to recall. Those skills possibly measured by the multiple phase Bender format are discussed and the need for research using the three-phase method is suggested."}
{"id": "PMID:461045", "title": "Role of verbal representations in testing recognition of naturalistic sounds.", "content": "This paper reports two experiments concerned with verbal representation in the test stage of recognition memory for naturalistic sounds. In Study I 10 subjects produced verbal description to 50 sounds and after a 7-day delay 5 subjects reviewed self-produced verbal descriptions while 5 did not prior to testing for recognition. Review of descriptions increased recognition to 94.4% compared to 88.5% for those with no review. In Study II 16 subjects produced verbal descripitions to 50 naturalistic sounds. After a 7-day delay all subjects reviewed descriptionss of one-half of the items which were \"targets\" and 8 subjects reviewed description of 25 \"non-targeted\" items. The latter condition produced interference, observed as a significant decrement in recognition from 90.3% to 86.5%.", "contents": "Role of verbal representations in testing recognition of naturalistic sounds. This paper reports two experiments concerned with verbal representation in the test stage of recognition memory for naturalistic sounds. In Study I 10 subjects produced verbal description to 50 sounds and after a 7-day delay 5 subjects reviewed self-produced verbal descriptions while 5 did not prior to testing for recognition. Review of descriptions increased recognition to 94.4% compared to 88.5% for those with no review. In Study II 16 subjects produced verbal descripitions to 50 naturalistic sounds. After a 7-day delay all subjects reviewed descriptionss of one-half of the items which were \"targets\" and 8 subjects reviewed description of 25 \"non-targeted\" items. The latter condition produced interference, observed as a significant decrement in recognition from 90.3% to 86.5%."}
{"id": "PMID:461046", "title": "Correlations among perceptual-motor ability, self-concept and reading achievement in early elementary grades.", "content": "This study investigated the correlation among measures of perceptual-motor abilities, self-concept, and reading achievement and determined whether perceptual-motor ability and self-concept can predict reading achievement in the early elementary grades. A total of 105 boys and 108 girls from the first, and second and third grades were tested on the stabilometer, a modified Minnesota Manual Dexterity Test, a tapping test, the Primary Self-concept Inventory, and the Science Research Associates Assessment Survey. Intercorrelations across grade levels tended to be low and nonsignificant. The multiple regression procedures yielded no strong predictions of reading achievement. These findings tended to confirm the specificity of perceptual-motor ability, self-concept, and reading achievement.", "contents": "Correlations among perceptual-motor ability, self-concept and reading achievement in early elementary grades. This study investigated the correlation among measures of perceptual-motor abilities, self-concept, and reading achievement and determined whether perceptual-motor ability and self-concept can predict reading achievement in the early elementary grades. A total of 105 boys and 108 girls from the first, and second and third grades were tested on the stabilometer, a modified Minnesota Manual Dexterity Test, a tapping test, the Primary Self-concept Inventory, and the Science Research Associates Assessment Survey. Intercorrelations across grade levels tended to be low and nonsignificant. The multiple regression procedures yielded no strong predictions of reading achievement. These findings tended to confirm the specificity of perceptual-motor ability, self-concept, and reading achievement."}
{"id": "PMID:461047", "title": "Restructuring ability and patterns of physics achievement.", "content": "For a sample of 24 male and 19 female college students, persons with high visualization (F1,36 = 7.60, P less than .01) and low flexibility of closure (F1,36 = 6.54, P less than .02) attained significantly higher achievement in physics than persons with low visualization or high flexibility of closure. Persons with high restructuring ability and high spatial visualization (M = 88.64, SD = 15.6) attained a significantly higher level of physics achievement than persons with high restructuring ability and low spatial visualization (M = 56.5, SD = 25.1). Flexibility of closure, spatial visualization, and the embedded figures test accounted for 23.8%, 18.4%, and 53% of the variability in physics achievement among the entire sample, among amles, and among females, respectively. The data provided moderate support for the field-dependence--independence model of psychological differentiation and for investigators who reported a possible link between field-dependence--independence, visualization, and extent of neurological lateralization.", "contents": "Restructuring ability and patterns of physics achievement. For a sample of 24 male and 19 female college students, persons with high visualization (F1,36 = 7.60, P less than .01) and low flexibility of closure (F1,36 = 6.54, P less than .02) attained significantly higher achievement in physics than persons with low visualization or high flexibility of closure. Persons with high restructuring ability and high spatial visualization (M = 88.64, SD = 15.6) attained a significantly higher level of physics achievement than persons with high restructuring ability and low spatial visualization (M = 56.5, SD = 25.1). Flexibility of closure, spatial visualization, and the embedded figures test accounted for 23.8%, 18.4%, and 53% of the variability in physics achievement among the entire sample, among amles, and among females, respectively. The data provided moderate support for the field-dependence--independence model of psychological differentiation and for investigators who reported a possible link between field-dependence--independence, visualization, and extent of neurological lateralization."}
{"id": "PMID:461048", "title": "Simple auditory reaction time in blind and sighted adolescents.", "content": "This study compared simple auditory reaction times of 20 subjects aged between 11 and 15 yr. (M = 13), 10 born-blind from the Louis Braille Institut (Montr\u00e9al) and 10 normal sighted subjects of the same age and sex. Their task was to press a telegraph key as fast as possible after presentation of an auditory stimulus. Each subject executed five blocks of 10 trials; the blocks were separated by a rest period of 1 min. The results showed no significant difference (p greater than .05) between born-blind and sighted people of the same sex and chronological age in regard to simple auditory reaction time.", "contents": "Simple auditory reaction time in blind and sighted adolescents. This study compared simple auditory reaction times of 20 subjects aged between 11 and 15 yr. (M = 13), 10 born-blind from the Louis Braille Institut (Montr\u00e9al) and 10 normal sighted subjects of the same age and sex. Their task was to press a telegraph key as fast as possible after presentation of an auditory stimulus. Each subject executed five blocks of 10 trials; the blocks were separated by a rest period of 1 min. The results showed no significant difference (p greater than .05) between born-blind and sighted people of the same sex and chronological age in regard to simple auditory reaction time."}
{"id": "PMID:461050", "title": "Field-dependence/field-independence and labeling of facial affect.", "content": "56 high school students were administered the Group Embedded Figures Test and the Pictures of Facial Affect. A low Pearson product-moment correlation of .02 was obtained between the measures. Data indicated that field-dependent and field-independent individuals were not signficantly different in their skills of labeling pictures of facial affect.", "contents": "Field-dependence/field-independence and labeling of facial affect. 56 high school students were administered the Group Embedded Figures Test and the Pictures of Facial Affect. A low Pearson product-moment correlation of .02 was obtained between the measures. Data indicated that field-dependent and field-independent individuals were not signficantly different in their skills of labeling pictures of facial affect."}
{"id": "PMID:461051", "title": "Effects of total sleep loss on sleep tendency.", "content": "Effects of two nights of sleep loss were assessed in six young adult (18--21 yr.) volunteers (2 women, 4 men). Performance on the Wilkinson Addition Test fell significantly below baseline values during the sleep-loss procedure and recovered after one or two full nights of sleep. Performance on a Serial Alternation Task also declined during sleep loss. Mood and sleepiness, assessed by subjective self-rating scales, showed a significantly less positive mood and a greater degree of sleepiness during sleep loss, with a recovery to baseline levels after one full night of sleep. Sleep tendency, measured at 2-hr. intervals during all waking periods, was assessed using an objective measure of latency to sleep onset, the Sleep Latency Test. The scores fell to about 1 min. at 0600 on the first night of sleep loss and remained at similarly low values throughout the sleep loss period. After one night of recovery sleep the scores remained significantly below baseline levels, which were not achieved until after the second recovery night. The multiple sleep latency test appears to be a valuable operationally defined tool for measuring daytime sleepiness.", "contents": "Effects of total sleep loss on sleep tendency. Effects of two nights of sleep loss were assessed in six young adult (18--21 yr.) volunteers (2 women, 4 men). Performance on the Wilkinson Addition Test fell significantly below baseline values during the sleep-loss procedure and recovered after one or two full nights of sleep. Performance on a Serial Alternation Task also declined during sleep loss. Mood and sleepiness, assessed by subjective self-rating scales, showed a significantly less positive mood and a greater degree of sleepiness during sleep loss, with a recovery to baseline levels after one full night of sleep. Sleep tendency, measured at 2-hr. intervals during all waking periods, was assessed using an objective measure of latency to sleep onset, the Sleep Latency Test. The scores fell to about 1 min. at 0600 on the first night of sleep loss and remained at similarly low values throughout the sleep loss period. After one night of recovery sleep the scores remained significantly below baseline levels, which were not achieved until after the second recovery night. The multiple sleep latency test appears to be a valuable operationally defined tool for measuring daytime sleepiness."}
{"id": "PMID:461052", "title": "Eye color and judgments of aesthetic value of shapes.", "content": "47 university students provided judgments of the aesthetic value of polygon and histogram shapes that were either symmetric or asymmetric. A sigificant over-all preference to symmetric shapes was evident (p less than .05), and judgments were significantly more positive for brown-eyed subjects. Furthermore, the aesthetic values assigned to shapes showed a systematic interaction with eye color such that brown-eyed subjects produced higher aesthetic ratings for symmetric shapes than blue-eyed subjects, while no such differences appeared for asymmetric shapes.", "contents": "Eye color and judgments of aesthetic value of shapes. 47 university students provided judgments of the aesthetic value of polygon and histogram shapes that were either symmetric or asymmetric. A sigificant over-all preference to symmetric shapes was evident (p less than .05), and judgments were significantly more positive for brown-eyed subjects. Furthermore, the aesthetic values assigned to shapes showed a systematic interaction with eye color such that brown-eyed subjects produced higher aesthetic ratings for symmetric shapes than blue-eyed subjects, while no such differences appeared for asymmetric shapes."}
{"id": "PMID:461053", "title": "Manifest anxiety and two-point threshold sensitivity.", "content": "The present study utilized 27 college students (14 females, 13 males) to investigate the effects of anxiety upon the ability to attend to detail (two-point threshold sensitivity). It was hypothesized that subjects both low and high in anxiety would have a smaller threshold than mild or moderately anxious subjects. A curvilinear correlation of .83 (p less .01) was obtained between anxiety scores and two-point threshold sensitivity. There was no significant difference between mean thresholds for those scoring above or below the median on the anxiety scale. Suggestions for further research are discussed.", "contents": "Manifest anxiety and two-point threshold sensitivity. The present study utilized 27 college students (14 females, 13 males) to investigate the effects of anxiety upon the ability to attend to detail (two-point threshold sensitivity). It was hypothesized that subjects both low and high in anxiety would have a smaller threshold than mild or moderately anxious subjects. A curvilinear correlation of .83 (p less .01) was obtained between anxiety scores and two-point threshold sensitivity. There was no significant difference between mean thresholds for those scoring above or below the median on the anxiety scale. Suggestions for further research are discussed."}
{"id": "PMID:461054", "title": "Individual differences in visual discrimination of letters.", "content": "Research on the distinctive features of letters has been inconclusive. Investigators may have overlooked individual variations in their data which would have supported feature-extraction hypotheses but were concealed by analyses which averaged data into group means. Response latencies from a matching discrimination task with the 26 English capital letters were analyzed with INDSCAL, a multidimensional scaling procedure which weights individual contributions to a psychological space. Underlying a dimensions were linearity, curvature, angularity, and closure. The solution disclosed that individuals utilized these features differentially in the visual discriminations. The results suggest that future research in visual feature extraction will benefit from the examination of individual variations in the perceptual process.", "contents": "Individual differences in visual discrimination of letters. Research on the distinctive features of letters has been inconclusive. Investigators may have overlooked individual variations in their data which would have supported feature-extraction hypotheses but were concealed by analyses which averaged data into group means. Response latencies from a matching discrimination task with the 26 English capital letters were analyzed with INDSCAL, a multidimensional scaling procedure which weights individual contributions to a psychological space. Underlying a dimensions were linearity, curvature, angularity, and closure. The solution disclosed that individuals utilized these features differentially in the visual discriminations. The results suggest that future research in visual feature extraction will benefit from the examination of individual variations in the perceptual process."}
{"id": "PMID:461055", "title": "A trimodal behavioral conceptualization of clinical pain.", "content": "A trimodal behavioral conceptualization of pain is outlined in the current article. It proposes that pain should be viewed as an interacting cluster of gross motor, cognitive, and physiological responses. Such a conceptual system is thought to contain the basic properties needed to incorporate current divergent views of pain held by medical and behavior science into a common conceptual framework.", "contents": "A trimodal behavioral conceptualization of clinical pain. A trimodal behavioral conceptualization of pain is outlined in the current article. It proposes that pain should be viewed as an interacting cluster of gross motor, cognitive, and physiological responses. Such a conceptual system is thought to contain the basic properties needed to incorporate current divergent views of pain held by medical and behavior science into a common conceptual framework."}
{"id": "PMID:461056", "title": "Effects of some social variables on field dependence.", "content": "The effects of socio-economic status, father's absence, family size, and birth order on the field dependence of Grade 6 elementary school children in Jamaica, West Indies, were examined. To 206 boys and 212 girls from 5 urban schools the Hidden Figures Test and a questionnaire were administered. For girls, but not for boys, field independence correlated significantly and positively with socio-economic status, but significantly and negatively with father's absence. No significant relation existed for the boys. Field dependence had no significant relation with family size and birth order.", "contents": "Effects of some social variables on field dependence. The effects of socio-economic status, father's absence, family size, and birth order on the field dependence of Grade 6 elementary school children in Jamaica, West Indies, were examined. To 206 boys and 212 girls from 5 urban schools the Hidden Figures Test and a questionnaire were administered. For girls, but not for boys, field independence correlated significantly and positively with socio-economic status, but significantly and negatively with father's absence. No significant relation existed for the boys. Field dependence had no significant relation with family size and birth order."}
{"id": "PMID:461057", "title": "Intrahemispheric visual-motor information processing and cerebral functional lateralization.", "content": "A series of stimuli, words and faces, were presented tachistoscopically to 24 dextrals and 12 sinistrals. The stimuli were presented to one eye at a time and the subjects were instructed to respond to specific words or stimuli with a specific hand. The results indicate that (1) cerebral functional asymmetry is related to handedness; in the dextrals, the left hemisphere is more specialized in verbal recognition, while in the sinistrals, the right hemisphere is more specialized in recognizing non-verbal material. (2) An ipsilateral hand-and-eye combination is a valid method of measuring intrahemispheric information processing, provided that the tachistoscopically presented visual stimuli are capable of inciting specialized hemispheric function. The dominant relationship among the crossed and non-crossed visual pathways is discussed.", "contents": "Intrahemispheric visual-motor information processing and cerebral functional lateralization. A series of stimuli, words and faces, were presented tachistoscopically to 24 dextrals and 12 sinistrals. The stimuli were presented to one eye at a time and the subjects were instructed to respond to specific words or stimuli with a specific hand. The results indicate that (1) cerebral functional asymmetry is related to handedness; in the dextrals, the left hemisphere is more specialized in verbal recognition, while in the sinistrals, the right hemisphere is more specialized in recognizing non-verbal material. (2) An ipsilateral hand-and-eye combination is a valid method of measuring intrahemispheric information processing, provided that the tachistoscopically presented visual stimuli are capable of inciting specialized hemispheric function. The dominant relationship among the crossed and non-crossed visual pathways is discussed."}
{"id": "PMID:461059", "title": "Relative locations among moving spots and visual vector analysis.", "content": "How the direction of apparent movement changed with the relative locations among spots was quantitatively measured by the method proposed by Hochberg and Fallon. From the vector-extraction model (proposed by Johansson) expanded as, \"The vector to be subtracted depends on the relative location.\" and the directions of apparent movement, the vector to be subtracted was calculated at each relative location. Consequently data showed (i) the region in which the directions of spots in motion are affected by the frame is finite, and (ii) the degree of the influence is not iniform in the region but is rapidly reduced as spots go away from the frame.", "contents": "Relative locations among moving spots and visual vector analysis. How the direction of apparent movement changed with the relative locations among spots was quantitatively measured by the method proposed by Hochberg and Fallon. From the vector-extraction model (proposed by Johansson) expanded as, \"The vector to be subtracted depends on the relative location.\" and the directions of apparent movement, the vector to be subtracted was calculated at each relative location. Consequently data showed (i) the region in which the directions of spots in motion are affected by the frame is finite, and (ii) the degree of the influence is not iniform in the region but is rapidly reduced as spots go away from the frame."}
{"id": "PMID:461060", "title": "A genetic explanation for no sex difference in spatial ability among Eskimos.", "content": "Superior spatial ability may be inherited as an X-linked recessive trait. Since the sex ratio for an X-linked recessive trait depends on the gene frequency of the recessive allele, the absence of a sex difference in Eskimo spatial ability may reflect an unusually high frequency of the recessive allele in the Eskimo population.", "contents": "A genetic explanation for no sex difference in spatial ability among Eskimos. Superior spatial ability may be inherited as an X-linked recessive trait. Since the sex ratio for an X-linked recessive trait depends on the gene frequency of the recessive allele, the absence of a sex difference in Eskimo spatial ability may reflect an unusually high frequency of the recessive allele in the Eskimo population."}
{"id": "PMID:461061", "title": "Visual-motor problems of adolescents who attempt suicide.", "content": "Using the Canter Background Interference Procedure with the Bender-Gestalt Test, a group of 18 adolescent suicide attempters earned test scores indicating they had significantly more problems with visual-motor coordination than did a control group of 21 adolescents. There were also a greater number of school failures and behavior problems among these suicide attempters. The findings suggest that learning disabilities may be an unrecognized factor which increases the risk of suicide attempts by adolescents.", "contents": "Visual-motor problems of adolescents who attempt suicide. Using the Canter Background Interference Procedure with the Bender-Gestalt Test, a group of 18 adolescent suicide attempters earned test scores indicating they had significantly more problems with visual-motor coordination than did a control group of 21 adolescents. There were also a greater number of school failures and behavior problems among these suicide attempters. The findings suggest that learning disabilities may be an unrecognized factor which increases the risk of suicide attempts by adolescents."}
{"id": "PMID:461062", "title": "Subjective hypotenuse estimation: a test of the Pythagorean theorem.", "content": "The Pythagorean theorem constitutes a normative model for the estimation of the length of the hypotenuse of a right-angled figure. Tested with functional measurement methods, this mathematically correct model was inappropriate as a descriptive model. The problem was an orientation effect; hypotenuse estimates for vertically oriented figures were larger than those for horizontally oriented figures composed of the same lengths. Because of this discrepancy, no additive model could describe the judgments.", "contents": "Subjective hypotenuse estimation: a test of the Pythagorean theorem. The Pythagorean theorem constitutes a normative model for the estimation of the length of the hypotenuse of a right-angled figure. Tested with functional measurement methods, this mathematically correct model was inappropriate as a descriptive model. The problem was an orientation effect; hypotenuse estimates for vertically oriented figures were larger than those for horizontally oriented figures composed of the same lengths. Because of this discrepancy, no additive model could describe the judgments."}
{"id": "PMID:461064", "title": "Eye movements simultaneously recorded by electrooculographic and photoelectric methods.", "content": "Three types of eye movements, saccadic, reading, and pursuit, were recorded from 6 college subjects, two in each by the electrooculographic and photoelectric methods simultaneously. A deviation index (DI), which is the standard deviation divided by the mean, was devised to compare the precision of recording amplitude deflection, and a proportion index (PI), which is ml divided by M2, was devised to compare the mean amplitude indirectly between these two methods. Results showed that the proportion indexes of three types of eye movements were comparable, and the mean index of 0.54 indicated that the amplification in the electrooculographic method was about half as much as that in the photoelectric. The mean deviation index of 0.132 vs 0.135 was, again, comparable, meaning that these two methods of recording amplitude deflections are of about the same degree of magnitude and precision. Certain qualitative differences regarding the amplitude and velocity peak deflection between these two methods were also noted.", "contents": "Eye movements simultaneously recorded by electrooculographic and photoelectric methods. Three types of eye movements, saccadic, reading, and pursuit, were recorded from 6 college subjects, two in each by the electrooculographic and photoelectric methods simultaneously. A deviation index (DI), which is the standard deviation divided by the mean, was devised to compare the precision of recording amplitude deflection, and a proportion index (PI), which is ml divided by M2, was devised to compare the mean amplitude indirectly between these two methods. Results showed that the proportion indexes of three types of eye movements were comparable, and the mean index of 0.54 indicated that the amplification in the electrooculographic method was about half as much as that in the photoelectric. The mean deviation index of 0.132 vs 0.135 was, again, comparable, meaning that these two methods of recording amplitude deflections are of about the same degree of magnitude and precision. Certain qualitative differences regarding the amplitude and velocity peak deflection between these two methods were also noted."}
{"id": "PMID:461065", "title": "Imagery rating and recall in congenitally blind subjects.", "content": "Imagery ratings, incidental free recall, and intentional free recall of a group of young congenitally blind adults living in an institute for the blind and of control group were compared. Words to be rated and recalled belonged to three categories: (a) high-imagery words whose referents can be sensorially experienced also by the blind; (b) high-imagery words whose referents cannot be experienced; and (c) low-imagery words. In the control group, both ratings of imagery and recall were affected by the category to which the word belonged. For the blind group the category of the word affected only the ratings of imagery which still assumed a peculiar form since words in category b received extremely low ratings. It is concluded that the blind can evaluate the imagery value of a word, but that their recall is not affected by the level of its imagery value. Therefore, the imagery value really seems to describe, as Paivio asserts, the susceptibility of an item to being coded in a specific visuo-imaginal way, which is more available for sighted than for blind people.", "contents": "Imagery rating and recall in congenitally blind subjects. Imagery ratings, incidental free recall, and intentional free recall of a group of young congenitally blind adults living in an institute for the blind and of control group were compared. Words to be rated and recalled belonged to three categories: (a) high-imagery words whose referents can be sensorially experienced also by the blind; (b) high-imagery words whose referents cannot be experienced; and (c) low-imagery words. In the control group, both ratings of imagery and recall were affected by the category to which the word belonged. For the blind group the category of the word affected only the ratings of imagery which still assumed a peculiar form since words in category b received extremely low ratings. It is concluded that the blind can evaluate the imagery value of a word, but that their recall is not affected by the level of its imagery value. Therefore, the imagery value really seems to describe, as Paivio asserts, the susceptibility of an item to being coded in a specific visuo-imaginal way, which is more available for sighted than for blind people."}
{"id": "PMID:461066", "title": "A test battery to assess intrasensory and intersensory development of young children.", "content": "The purpose of the study was to develop a battery of tests for use in evaluation of intra- and intersensory development of young children. A battery of 15 tests (4 visual, 4 auditory, 4 tactile-kinesthetic, and 3 intersensory) was administered to 109 normally developing 6- and 8-year-old and 32 slowly developing or learning disabled children. Interdependence of test items within each intrasensory and the intersensory category was determined; intercorrelations ranged from .00 to .78. Reliability estimates were also determined. Face validity was claimed for each item. The effects of age or developmental level on test performance were established. Based upon the interdependence of the tests, reliability estimates, and the capacity of the tests to discriminate among groups classified according to age or developmental level, a battery of 10 intra- and intersensory tests was proposed. The battery has 3 tests of visual perception-visual memory, dynamic depth perception, and size discrimination; 3 tests of auditory perception-auditory discrimination, auditory memory of related syllables, and auditory sequential memory of numbers; 2 tests of tactile-kinesthetic perception-tactile integration and movement awareness; and 2 tests of intersensory integration-auditory-tactile intergration and auditory-visual integration.", "contents": "A test battery to assess intrasensory and intersensory development of young children. The purpose of the study was to develop a battery of tests for use in evaluation of intra- and intersensory development of young children. A battery of 15 tests (4 visual, 4 auditory, 4 tactile-kinesthetic, and 3 intersensory) was administered to 109 normally developing 6- and 8-year-old and 32 slowly developing or learning disabled children. Interdependence of test items within each intrasensory and the intersensory category was determined; intercorrelations ranged from .00 to .78. Reliability estimates were also determined. Face validity was claimed for each item. The effects of age or developmental level on test performance were established. Based upon the interdependence of the tests, reliability estimates, and the capacity of the tests to discriminate among groups classified according to age or developmental level, a battery of 10 intra- and intersensory tests was proposed. The battery has 3 tests of visual perception-visual memory, dynamic depth perception, and size discrimination; 3 tests of auditory perception-auditory discrimination, auditory memory of related syllables, and auditory sequential memory of numbers; 2 tests of tactile-kinesthetic perception-tactile integration and movement awareness; and 2 tests of intersensory integration-auditory-tactile intergration and auditory-visual integration."}
{"id": "PMID:461067", "title": "Reliability and validity of the Group Embedded Figures Test for a grade school sample.", "content": "Data on validity of the Group Embedded Figures Test for 22 sixth grade students (9 boys and 13 girls) were obtained. 50 wk. later all subjects were individually screened on the Portable Rod-and-Frame Test and after a period of 2 more weeks, when they were retested on the embedded figures, data on reliability were obtained. Long-term coefficients of stability of .80 and .71 for the 9 boys and 13 girls, respectively, were obtained. Coefficients of internal consistency ranged from .83 to .98 for boys and girls. Coefficients reflecting both construct and concurrent validity were -.60 and .00 for boys and girls, respectively. Customary sex differences with respect to field-dependence--independence were not observed for these small samples.", "contents": "Reliability and validity of the Group Embedded Figures Test for a grade school sample. Data on validity of the Group Embedded Figures Test for 22 sixth grade students (9 boys and 13 girls) were obtained. 50 wk. later all subjects were individually screened on the Portable Rod-and-Frame Test and after a period of 2 more weeks, when they were retested on the embedded figures, data on reliability were obtained. Long-term coefficients of stability of .80 and .71 for the 9 boys and 13 girls, respectively, were obtained. Coefficients of internal consistency ranged from .83 to .98 for boys and girls. Coefficients reflecting both construct and concurrent validity were -.60 and .00 for boys and girls, respectively. Customary sex differences with respect to field-dependence--independence were not observed for these small samples."}
{"id": "PMID:461068", "title": "Individual difference in anticipatory physiological responses to signaled stress: role of locus of control and autonomic balance.", "content": "Anticipatory cardiac activity to a signaled stressful event was hypothesized to be related to individual differences in locus of control and to autonomic balance. Subjects experienced 5 trials of a 105-db burst of white noise that was signaled by a milder tone. Significant increases in electrodermal as well as heart-rate responses occurred after the stress tone. While anticipating stress, the 10 subjects designated as having high levels of coherence between heart-rate and respiratory activity (high parasympathetic balance) showed reduced heart-rate and respiratory activity (high parasympathetic balance) showed reduced heart-rate variability over the 10 subjects with low coherence. Locus of control was not related to anticipatory activity. Anticipatory cardiac activity is discussed in light of two current psychophysiological models of attention.", "contents": "Individual difference in anticipatory physiological responses to signaled stress: role of locus of control and autonomic balance. Anticipatory cardiac activity to a signaled stressful event was hypothesized to be related to individual differences in locus of control and to autonomic balance. Subjects experienced 5 trials of a 105-db burst of white noise that was signaled by a milder tone. Significant increases in electrodermal as well as heart-rate responses occurred after the stress tone. While anticipating stress, the 10 subjects designated as having high levels of coherence between heart-rate and respiratory activity (high parasympathetic balance) showed reduced heart-rate and respiratory activity (high parasympathetic balance) showed reduced heart-rate variability over the 10 subjects with low coherence. Locus of control was not related to anticipatory activity. Anticipatory cardiac activity is discussed in light of two current psychophysiological models of attention."}
{"id": "PMID:461070", "title": "Generalization gradients following differential intradimensional autoshaping.", "content": "Three pigeons were trained on a differential, intradimensional autoshaped discrimination. A 45 degrees line tilt was always paired with food whereas a 15 degrees line tilt was never paired with food. All subjects learned the discrimination within 17 sessions. The pigeons were then given generalization tests in extinction over seven line tilts (0 degrees, 15 degrees, 30 degrees, 45 degrees, 60 degrees, 75 degrees and 90 degrees). The subjects yielded generalization gradients with maxima at 45 degrees and minima at 15 degrees. An area shift, but no peak shift, was found for each subject.", "contents": "Generalization gradients following differential intradimensional autoshaping. Three pigeons were trained on a differential, intradimensional autoshaped discrimination. A 45 degrees line tilt was always paired with food whereas a 15 degrees line tilt was never paired with food. All subjects learned the discrimination within 17 sessions. The pigeons were then given generalization tests in extinction over seven line tilts (0 degrees, 15 degrees, 30 degrees, 45 degrees, 60 degrees, 75 degrees and 90 degrees). The subjects yielded generalization gradients with maxima at 45 degrees and minima at 15 degrees. An area shift, but no peak shift, was found for each subject."}
{"id": "PMID:461112", "title": "Acephate and methamidophos residue behavior in Florida citrus, 1976.", "content": "The half-life of acephate and its hydrolysate, methamidophos, in the rind of Temple and Valencia oranges, and grapefruit, lemons, and tangerines was 10.3 days and 10.5 days, respectively. Half-lives of acephate and methamidophos in citrus pulp were 15.0 days and 6.1 days, respectively based on 7-, 14-, and 21-day data. Seven days after treatment, acephate and methamidophos reached maximum levels in rind and pulp. Acephate residue levels in rind were less than 3.0 ppm 14 days after treatment; acephate residues in pulp were less than 3.0 ppm throughout the experiment. Methamidophos residue levels averaged less than 0.25 ppm after 21 days.", "contents": "Acephate and methamidophos residue behavior in Florida citrus, 1976. The half-life of acephate and its hydrolysate, methamidophos, in the rind of Temple and Valencia oranges, and grapefruit, lemons, and tangerines was 10.3 days and 10.5 days, respectively. Half-lives of acephate and methamidophos in citrus pulp were 15.0 days and 6.1 days, respectively based on 7-, 14-, and 21-day data. Seven days after treatment, acephate and methamidophos reached maximum levels in rind and pulp. Acephate residue levels in rind were less than 3.0 ppm 14 days after treatment; acephate residues in pulp were less than 3.0 ppm throughout the experiment. Methamidophos residue levels averaged less than 0.25 ppm after 21 days."}
{"id": "PMID:461113", "title": "Effects of organochlorine residues on eggshell thickness, reproduction, and population status of brown pelicans (Pelecanus occidentalis) in South Carolina and Florida, 1969--76.", "content": "Shells of brown pelican (Pelecanus occidentalis) eggs collected in South Carolina from 1969 through 1975 and in Florida during 1969, 1970, and 1974 were significantly thinner (P greater than 0.05) than eggshells collected before 1947. Thickness of South Carolina eggshells increased in 1975, and mean thickness of eggshells collected in Florida during 1974 was greater than that of eggshells collected during 1969 and 1970, primarily in Gulf Coast colonies. Residues of 13 organochlorines were found in eggs and tissues of pelicans found dead during 1974 and 1975, although residues in brains of these specimens were not high enough to cause death. Residues of organochlorines, except PCBs, declined through 1975. PCBs increased in eggs from Atlantic Coast colonies. Reproductive success and population status of brown pelicans in South Carolina have improved markedly since authors began their studies in 1969. Good reproductive success was reported in 3 of 5 years from 1973 through 1977.", "contents": "Effects of organochlorine residues on eggshell thickness, reproduction, and population status of brown pelicans (Pelecanus occidentalis) in South Carolina and Florida, 1969--76. Shells of brown pelican (Pelecanus occidentalis) eggs collected in South Carolina from 1969 through 1975 and in Florida during 1969, 1970, and 1974 were significantly thinner (P greater than 0.05) than eggshells collected before 1947. Thickness of South Carolina eggshells increased in 1975, and mean thickness of eggshells collected in Florida during 1974 was greater than that of eggshells collected during 1969 and 1970, primarily in Gulf Coast colonies. Residues of 13 organochlorines were found in eggs and tissues of pelicans found dead during 1974 and 1975, although residues in brains of these specimens were not high enough to cause death. Residues of organochlorines, except PCBs, declined through 1975. PCBs increased in eggs from Atlantic Coast colonies. Reproductive success and population status of brown pelicans in South Carolina have improved markedly since authors began their studies in 1969. Good reproductive success was reported in 3 of 5 years from 1973 through 1977."}
{"id": "PMID:461114", "title": "Pesticide contamination of water rats in the Murrumbidgee Irrigation Areas, New South Wales, Australia, 1970-72.", "content": "Organochlorine pesticides were found in all samples of livers, kidneys, mammary glands, and fetuses of eastern water rats (Hydromys chrysogaster) collected in the Murrumbidgee irrigation areas of New South Wales in 1970 and 1972. DDE was the predominant residue. Livers contained 0.01--3.10 ppm sigma DDT air-dried weight; kidneys, less than 0.01--1.12 ppm; mammary glands, 0.14--23.75 ppm; fetal liver, 0.28--0.66 ppm. Variations in residue levels are discussed in relation to the possible effects of environmental and physiological factors.", "contents": "Pesticide contamination of water rats in the Murrumbidgee Irrigation Areas, New South Wales, Australia, 1970-72. Organochlorine pesticides were found in all samples of livers, kidneys, mammary glands, and fetuses of eastern water rats (Hydromys chrysogaster) collected in the Murrumbidgee irrigation areas of New South Wales in 1970 and 1972. DDE was the predominant residue. Livers contained 0.01--3.10 ppm sigma DDT air-dried weight; kidneys, less than 0.01--1.12 ppm; mammary glands, 0.14--23.75 ppm; fetal liver, 0.28--0.66 ppm. Variations in residue levels are discussed in relation to the possible effects of environmental and physiological factors."}
{"id": "PMID:461115", "title": "Pesticide application and cropping data from 37 states, 1972--national soils monitoring program.", "content": "This report summarizes pesticide application and cropping data collected in 1972 from 1,402 agricultural sampling sites in 37 states as part of the National Soils Monitoring Program. Pesticide application data are summarized by all sites, states, and crop. Tables generally give the number of sites reporting, number of times a compound was applied, percent occurrence, and arithmetic mean application rate. Pesticides applied most frequently were atrazine, 2.4-D, captan, and trifluralin. Among selected major crops, pesticides were most frequently applied to sites growing field corn and cotton, least frequently to sites growing alfalfa/bur clover and mixed hay.", "contents": "Pesticide application and cropping data from 37 states, 1972--national soils monitoring program. This report summarizes pesticide application and cropping data collected in 1972 from 1,402 agricultural sampling sites in 37 states as part of the National Soils Monitoring Program. Pesticide application data are summarized by all sites, states, and crop. Tables generally give the number of sites reporting, number of times a compound was applied, percent occurrence, and arithmetic mean application rate. Pesticides applied most frequently were atrazine, 2.4-D, captan, and trifluralin. Among selected major crops, pesticides were most frequently applied to sites growing field corn and cotton, least frequently to sites growing alfalfa/bur clover and mixed hay."}
{"id": "PMID:461116", "title": "Pesticide residue levels in soils and crops from 37 states, 1972--National Soils Monitoring Program (IV).", "content": "Residue data from the 1972 (FY--73) National Soils Monitoring Program are summarized. Composite samples of agricultural soil and mature crops were collected from 1,483 of the 1,533 selected 4-hectare sites in 37 states. Analyses were performed for organochlorine and organophosphorus compounds, trifluralin and polychlorinated biphenyls (PCBs); analysis for atrazine was performed only when pesticide application data indicated current-year use. Organochlorine pesticides were detected in 45 percent of the soil samples. The most frequently detected compound was dieldrin, found in 27 percent of all soil samples. Other compounds detected, in order of frequency, included DDT, aldrin, chlordane, and heptachlor epoxide, found, respectively, in 21, 9, 8, and 7 percent of all soil samples. Crop samples were collected from 727 sites. All were analyzed for organochlorines; analyses were performed for organophosphates and atrazine only when pesticide application data indicated current-year use. For all crops, 40 percent of the samples contained detectable levels of organochlorines and 10 percent contained detectable levels of organophosphates. Atrazine was not detected.", "contents": "Pesticide residue levels in soils and crops from 37 states, 1972--National Soils Monitoring Program (IV). Residue data from the 1972 (FY--73) National Soils Monitoring Program are summarized. Composite samples of agricultural soil and mature crops were collected from 1,483 of the 1,533 selected 4-hectare sites in 37 states. Analyses were performed for organochlorine and organophosphorus compounds, trifluralin and polychlorinated biphenyls (PCBs); analysis for atrazine was performed only when pesticide application data indicated current-year use. Organochlorine pesticides were detected in 45 percent of the soil samples. The most frequently detected compound was dieldrin, found in 27 percent of all soil samples. Other compounds detected, in order of frequency, included DDT, aldrin, chlordane, and heptachlor epoxide, found, respectively, in 21, 9, 8, and 7 percent of all soil samples. Crop samples were collected from 727 sites. All were analyzed for organochlorines; analyses were performed for organophosphates and atrazine only when pesticide application data indicated current-year use. For all crops, 40 percent of the samples contained detectable levels of organochlorines and 10 percent contained detectable levels of organophosphates. Atrazine was not detected."}
{"id": "PMID:461117", "title": "Organochlorine pesticide residues in soils from six U.S. Air Force bases, 1975--76.", "content": "Soil samples collected during 1975 and 1976 from United States Air Force installations in California, Georgia, Ohio, Oklahoma, Texas, and Utah were analyzed for organochlorine pesticide residues. Sigma DDT, chlordane, and dieldrin were the pesticides most commonly found. In 1975, sigma DDT residues were significantly higher in samples from residential areas than in samples from golf courses or areas free of pesticide application. Chlordane residues in 1975 were significantly higher in both residential and golf course areas than in areas where pesticides had not been used. No significant differences were found in 1976 in residue levels of any pesticide monitored among various land use areas.", "contents": "Organochlorine pesticide residues in soils from six U.S. Air Force bases, 1975--76. Soil samples collected during 1975 and 1976 from United States Air Force installations in California, Georgia, Ohio, Oklahoma, Texas, and Utah were analyzed for organochlorine pesticide residues. Sigma DDT, chlordane, and dieldrin were the pesticides most commonly found. In 1975, sigma DDT residues were significantly higher in samples from residential areas than in samples from golf courses or areas free of pesticide application. Chlordane residues in 1975 were significantly higher in both residential and golf course areas than in areas where pesticides had not been used. No significant differences were found in 1976 in residue levels of any pesticide monitored among various land use areas."}
{"id": "PMID:461118", "title": "[Clinical use of ketamine-xylazine for anaesthesia in the cat (author's transl)].", "content": "Based on experiences from anaesthesia of approximately 7000 cats in a four year period the effects of a combination of 20--25 mg/kg Ketamine and 0.5 mg/kg Xylazine given i/m are described. In the present study the Xylazine has been applied in considerably lower doses, compared with previous reports on these drugs, and this change has reduced the unwanted side effects, without at the same time reducing the effect on the muscular tension and the psychical disturbances induced by the Ketamine. Ketamine and Xylazine were given in one injection after being taken in the named sequence and mixed in the syringe. Indication for anaesthetizing the cats were, besides routine surgery in the out-patient clinic, operations of weakened animals for pyometra, foreign bodies, intestinal invaginations with and without resection, removal of abdominal tumors and urolithiasis. Animals with impared liver function were not anaesthetized with these drugs due to the important role of liver metabolism in their excretion. In spite of the fact that the corneal and laryngeal reflexes normally persist, the combination of the two drugs allowed surgery in these organs after application of local anaesthetics as an extra precaution. Premedication with atropine has not been used routinely, and still only very few cases of increased salivation or vomiting have been observed. Aspiration has not been a complication and in the whole material, only 3 deaths have occurred, none of them with a specific post mortem finding besides shock. In these 3 cases the patient died later than 45 minutes after the injection and after ended surgery. Side effects ascribed to phenomena of interaction have not been observed. One cat was anaesthetized a number of times during pregnancy without any effect on the cat or its kittens. It is concluded, that the Ketamine/Xylazine combination, when mixed as prescribed gives a very safe and pleasant narcosis, and that side effects are minimized, if the corneas are moistened with an ophthalmic ointment and the patient is allowed to recover in dark and quiet surroundings.", "contents": "[Clinical use of ketamine-xylazine for anaesthesia in the cat (author's transl)]. Based on experiences from anaesthesia of approximately 7000 cats in a four year period the effects of a combination of 20--25 mg/kg Ketamine and 0.5 mg/kg Xylazine given i/m are described. In the present study the Xylazine has been applied in considerably lower doses, compared with previous reports on these drugs, and this change has reduced the unwanted side effects, without at the same time reducing the effect on the muscular tension and the psychical disturbances induced by the Ketamine. Ketamine and Xylazine were given in one injection after being taken in the named sequence and mixed in the syringe. Indication for anaesthetizing the cats were, besides routine surgery in the out-patient clinic, operations of weakened animals for pyometra, foreign bodies, intestinal invaginations with and without resection, removal of abdominal tumors and urolithiasis. Animals with impared liver function were not anaesthetized with these drugs due to the important role of liver metabolism in their excretion. In spite of the fact that the corneal and laryngeal reflexes normally persist, the combination of the two drugs allowed surgery in these organs after application of local anaesthetics as an extra precaution. Premedication with atropine has not been used routinely, and still only very few cases of increased salivation or vomiting have been observed. Aspiration has not been a complication and in the whole material, only 3 deaths have occurred, none of them with a specific post mortem finding besides shock. In these 3 cases the patient died later than 45 minutes after the injection and after ended surgery. Side effects ascribed to phenomena of interaction have not been observed. One cat was anaesthetized a number of times during pregnancy without any effect on the cat or its kittens. It is concluded, that the Ketamine/Xylazine combination, when mixed as prescribed gives a very safe and pleasant narcosis, and that side effects are minimized, if the corneas are moistened with an ophthalmic ointment and the patient is allowed to recover in dark and quiet surroundings."}
{"id": "PMID:461119", "title": "Clinical manifestations of overdose of ketamine-xylazine in the cat.", "content": "10 cats were anesthetized with high doses of Ketamine/Xylazine combination (50 mg/kg and 6 mg per cat respectively). 10 other cats were given Ketamine alone (100 mg/kg). The drugs were given i/m. Heart rate and respiratory rate were measured with regular intervals for 3 hours and certain reflexes were checked in the same period. Blood parameters (pH, pO2, pCO2) from arterial blood, were studied 10--15 min. after application. The values measured after application of these high doses never reached critical levels and no clinical signs of cyanosis were observed. Physostigmine has been reported to have an antagonistic effect on Ketamine in humans, but this could not be demonstrated in cats. The increase of dose levels did not improve or lengthen the anaesthetic effect of the drugs, but the period of recovery was highly extended. There were no clinical signs of acute toxicity and it is concluded that even severe misjudgements of the bodyweight should not lead to fatal results.", "contents": "Clinical manifestations of overdose of ketamine-xylazine in the cat. 10 cats were anesthetized with high doses of Ketamine/Xylazine combination (50 mg/kg and 6 mg per cat respectively). 10 other cats were given Ketamine alone (100 mg/kg). The drugs were given i/m. Heart rate and respiratory rate were measured with regular intervals for 3 hours and certain reflexes were checked in the same period. Blood parameters (pH, pO2, pCO2) from arterial blood, were studied 10--15 min. after application. The values measured after application of these high doses never reached critical levels and no clinical signs of cyanosis were observed. Physostigmine has been reported to have an antagonistic effect on Ketamine in humans, but this could not be demonstrated in cats. The increase of dose levels did not improve or lengthen the anaesthetic effect of the drugs, but the period of recovery was highly extended. There were no clinical signs of acute toxicity and it is concluded that even severe misjudgements of the bodyweight should not lead to fatal results."}
{"id": "PMID:461120", "title": "[The microflora in cosmetics and non-sterile pharmaceuticals, and the microbiological standards for these products. I. A case of waterborne contamination of a hand-lotion (author's transl)].", "content": "A current case of a dynamic contamination of hand lotion with Pseudomonas cepacia is described. The Ps. cepacia occurred in the hand-lotion as a pure culture in a number of 10(4) bacteria per ml hand-lotion. The contaminant, originating from the tap-water, formed a preliminary reservoir in the deionizer, passed the sterile-filter and entered via the sterile water into the production system, where it formed a secondary reservoir in the top of one of the mixing units.", "contents": "[The microflora in cosmetics and non-sterile pharmaceuticals, and the microbiological standards for these products. I. A case of waterborne contamination of a hand-lotion (author's transl)]. A current case of a dynamic contamination of hand lotion with Pseudomonas cepacia is described. The Ps. cepacia occurred in the hand-lotion as a pure culture in a number of 10(4) bacteria per ml hand-lotion. The contaminant, originating from the tap-water, formed a preliminary reservoir in the deionizer, passed the sterile-filter and entered via the sterile water into the production system, where it formed a secondary reservoir in the top of one of the mixing units."}
{"id": "PMID:461121", "title": "[Clinical value of the determination of carcinoembryonic antigen in bronchial secretion (author's transl)].", "content": "Determination of carcinoembryonic antigen (CEA) by radioimmunoassay was performed in per-endoscopic obtained bronchial secretion. Bronchial CEA values are higher in the case of primary carcinoma of the lung than of metastatic lung cancer or pulmonary benign disease (p less than 0.0005). More interesting is the ratio bronchial CEA/serum CEA which is much higher in primary carcinoma of the lung than in benign disease (p less than 0.0005) and lower in metastatic lung carcinoma than benign disease (p less than 0.0025). In primary carcinoma of the lung, the CEA determination seems to have a prognostic value since higher levels are reported in patients unsuccessfully treated than in treated patients with apparent remission.", "contents": "[Clinical value of the determination of carcinoembryonic antigen in bronchial secretion (author's transl)]. Determination of carcinoembryonic antigen (CEA) by radioimmunoassay was performed in per-endoscopic obtained bronchial secretion. Bronchial CEA values are higher in the case of primary carcinoma of the lung than of metastatic lung cancer or pulmonary benign disease (p less than 0.0005). More interesting is the ratio bronchial CEA/serum CEA which is much higher in primary carcinoma of the lung than in benign disease (p less than 0.0005) and lower in metastatic lung carcinoma than benign disease (p less than 0.0025). In primary carcinoma of the lung, the CEA determination seems to have a prognostic value since higher levels are reported in patients unsuccessfully treated than in treated patients with apparent remission."}
{"id": "PMID:461132", "title": "[Diagnostic of left-to-right shunt by isotopic angiocardiography (author's transl)].", "content": "Isotopic angiocardiography was performed in 60 patients with proven congenital left-to-right shunt. A 99mTc labeled bolus was injected in a peripheral vein, and the first passage in the heart cavities was studied. Time-activity curves of the cardiac chambers were analysed, using a scintillation camera and a data processing system. In our study, we were able to confirm the presence of the shunt in 77% of the cases in which the radioactive bolus arrived well grouped in the vena cava. Quantitative results however remain imprecise. Isotopic angiocardiography indications are compared to radiologic angiocardiography and to right heart catheterism indications. Use of this non invasive technique could render more aggressive examinations unnecessary.", "contents": "[Diagnostic of left-to-right shunt by isotopic angiocardiography (author's transl)]. Isotopic angiocardiography was performed in 60 patients with proven congenital left-to-right shunt. A 99mTc labeled bolus was injected in a peripheral vein, and the first passage in the heart cavities was studied. Time-activity curves of the cardiac chambers were analysed, using a scintillation camera and a data processing system. In our study, we were able to confirm the presence of the shunt in 77% of the cases in which the radioactive bolus arrived well grouped in the vena cava. Quantitative results however remain imprecise. Isotopic angiocardiography indications are compared to radiologic angiocardiography and to right heart catheterism indications. Use of this non invasive technique could render more aggressive examinations unnecessary."}
{"id": "PMID:461133", "title": "[Massive medullary forms of Hodgkin's disease and acute myelofibrosis (author's transl)].", "content": "Ten cases of Hodgkin's disease was massive bone marrow invasion. This particular form of the disease is seen either at the outset or in Stage IIIB with a rapid course. It is reflected by a pancytopaenia with poor marrow with little or no superficial nodes. These very atypical forms of Hodgkin's disease must thus always be considered as a possibility in the context of acute malignant myelofibrosis. They indicate a special and unexplained behaviour of the host to the Hodgkin's tumour.", "contents": "[Massive medullary forms of Hodgkin's disease and acute myelofibrosis (author's transl)]. Ten cases of Hodgkin's disease was massive bone marrow invasion. This particular form of the disease is seen either at the outset or in Stage IIIB with a rapid course. It is reflected by a pancytopaenia with poor marrow with little or no superficial nodes. These very atypical forms of Hodgkin's disease must thus always be considered as a possibility in the context of acute malignant myelofibrosis. They indicate a special and unexplained behaviour of the host to the Hodgkin's tumour."}
{"id": "PMID:461134", "title": "[Prevention of hospital-acquired infection. Efficacy of isolation procedures in a neurological intensive care unit (ICU) (author's transl)].", "content": "Isolation procedures were adopted after usual measures failed to control hospital-acquired infection in a neurological ICU. All patients with an intubation or a tracheostomy were treated in individual rooms following the rules of strict isolation. The circulation of contaminated equipments was strictly isolated from the rest of the ICU. The efficacy of this isolation policy was aasessed by comparing the rates of hospital-acquired infections during the year before and the year after its adoption. The rates of pulmonary infections and venous catheters infections were significantly reduced (p less than or equal to 0.001). The rates of septicemias and urinary infections were relatively unaffected. The mortality caused by nosocomial infections showed a 64% fall (p less than 0.001) which accounted for a 29% reduction in the overall mortality (p less than 0.05). The average stay in the ICU was shortened by 20% and antibiotics consumption declined by 64% leading to substantial savings of money. The activity of the ICU as reflected by the number of admitted patients was unaffected.", "contents": "[Prevention of hospital-acquired infection. Efficacy of isolation procedures in a neurological intensive care unit (ICU) (author's transl)]. Isolation procedures were adopted after usual measures failed to control hospital-acquired infection in a neurological ICU. All patients with an intubation or a tracheostomy were treated in individual rooms following the rules of strict isolation. The circulation of contaminated equipments was strictly isolated from the rest of the ICU. The efficacy of this isolation policy was aasessed by comparing the rates of hospital-acquired infections during the year before and the year after its adoption. The rates of pulmonary infections and venous catheters infections were significantly reduced (p less than or equal to 0.001). The rates of septicemias and urinary infections were relatively unaffected. The mortality caused by nosocomial infections showed a 64% fall (p less than 0.001) which accounted for a 29% reduction in the overall mortality (p less than 0.05). The average stay in the ICU was shortened by 20% and antibiotics consumption declined by 64% leading to substantial savings of money. The activity of the ICU as reflected by the number of admitted patients was unaffected."}
{"id": "PMID:461135", "title": "[Progressive dialytic encephalopathy. Role of the aluminium and neurological study. One case (author's transl)].", "content": "Report a typical case of dialytic dementia in a patient treated with aluminium gels. The course was fatal in fifteen months duration. Before interruption of aluminium gel intake, the aluminium blood level measured by atomic absorption spectrography was at 1300 microgram/l (normal less than 40 microgram/l). Cerebral aluminium was studied by the method of Le Gendre and Alfrey. On the three studied specimens of gray matter including, the parieto rolandic cortex, the thalamus, the cerebellar cortex, the mean aluminium concentration was seven times higher than the witness. The optic and electronic microscopy study showed important accumulation of lipofuscin. No neurofibrillary degeneration was observed. In contrast to the intensity of the clinical signs and the fatal course the cerebral lesions were slight.", "contents": "[Progressive dialytic encephalopathy. Role of the aluminium and neurological study. One case (author's transl)]. Report a typical case of dialytic dementia in a patient treated with aluminium gels. The course was fatal in fifteen months duration. Before interruption of aluminium gel intake, the aluminium blood level measured by atomic absorption spectrography was at 1300 microgram/l (normal less than 40 microgram/l). Cerebral aluminium was studied by the method of Le Gendre and Alfrey. On the three studied specimens of gray matter including, the parieto rolandic cortex, the thalamus, the cerebellar cortex, the mean aluminium concentration was seven times higher than the witness. The optic and electronic microscopy study showed important accumulation of lipofuscin. No neurofibrillary degeneration was observed. In contrast to the intensity of the clinical signs and the fatal course the cerebral lesions were slight."}
{"id": "PMID:461136", "title": "[Idiopathic haemochromatosis. Pathogenic and genetic aspects. Detection and prevention (author's transl)].", "content": "The basic disorder of iron metabolism in idiopathic haemochromatosis finds expression on at least two levels: the intestinal mucosa (increased iron absorption) and the liver. Its exact nature, however, remains obscure. The role of iron overload in the pathogenesis of the disorder seems clear. Lysosome disruption has recently been proposed as a possible pathogenic factor. Phenotypic family studies have lent considerable weight to the hypothesis of a recessive transmission of idiopathic haemochromatosis. Demonstration of a close link between the disease and the HLA antigen A3 and haplotype A3, B14 has made it possible: to remove all doubt as to the hereditary nature of the disease; identify the underlying gene as located on chromosome 6 near the A locus of the HLA system; demonstrate a recessive mode of transmission; and achieve the early detection of individuals at risk in the family of a patient with the disease. Thanks to this possibility of early detection, the feasability of preventive measures is greatly enhanced.", "contents": "[Idiopathic haemochromatosis. Pathogenic and genetic aspects. Detection and prevention (author's transl)]. The basic disorder of iron metabolism in idiopathic haemochromatosis finds expression on at least two levels: the intestinal mucosa (increased iron absorption) and the liver. Its exact nature, however, remains obscure. The role of iron overload in the pathogenesis of the disorder seems clear. Lysosome disruption has recently been proposed as a possible pathogenic factor. Phenotypic family studies have lent considerable weight to the hypothesis of a recessive transmission of idiopathic haemochromatosis. Demonstration of a close link between the disease and the HLA antigen A3 and haplotype A3, B14 has made it possible: to remove all doubt as to the hereditary nature of the disease; identify the underlying gene as located on chromosome 6 near the A locus of the HLA system; demonstrate a recessive mode of transmission; and achieve the early detection of individuals at risk in the family of a patient with the disease. Thanks to this possibility of early detection, the feasability of preventive measures is greatly enhanced."}
{"id": "PMID:461144", "title": "[Valgisation osteotomy in the treatment of osteoarthrosis of the hip: 21 cases (author's transl)].", "content": "Study of 21 cases of femoral valgisation osteotomy, with a minimum follow-up of 5 years, confirms the value of this operation in the treatment of certain types of osteoarthrosis of the hip, and above all those types in which there is an inferior capital osteophyte. The indications depend upon: --clinical features: as in the case of all osteotomies, mobility must be at least equal to 70 degrees flexion. --radiological findings: reposition films in adduction should show widening of the joint space and above all improved congruence. The presence of geodes and lack of cover of the femoral head are not, in themselves, contraindications. The operation involves, in addition to valgisation inter-trochanteric osteotomy, relaxation of the peri-articular muscles (psoas, adductors, gluteus medius) in order that the relative lengthening of the upper end of the femur related to ventricalisation of the neck should not be in contradiction with the usual principles of surgery for osteoarthrosis. Valgisation osteotomy results in an increase in weight-bearing surfaces and thus a decrease in unit pressure. Indicated and performed in this way, this operation gives highly satisfactory and durable results, as shown by the follow-up period of our own cases.", "contents": "[Valgisation osteotomy in the treatment of osteoarthrosis of the hip: 21 cases (author's transl)]. Study of 21 cases of femoral valgisation osteotomy, with a minimum follow-up of 5 years, confirms the value of this operation in the treatment of certain types of osteoarthrosis of the hip, and above all those types in which there is an inferior capital osteophyte. The indications depend upon: --clinical features: as in the case of all osteotomies, mobility must be at least equal to 70 degrees flexion. --radiological findings: reposition films in adduction should show widening of the joint space and above all improved congruence. The presence of geodes and lack of cover of the femoral head are not, in themselves, contraindications. The operation involves, in addition to valgisation inter-trochanteric osteotomy, relaxation of the peri-articular muscles (psoas, adductors, gluteus medius) in order that the relative lengthening of the upper end of the femur related to ventricalisation of the neck should not be in contradiction with the usual principles of surgery for osteoarthrosis. Valgisation osteotomy results in an increase in weight-bearing surfaces and thus a decrease in unit pressure. Indicated and performed in this way, this operation gives highly satisfactory and durable results, as shown by the follow-up period of our own cases."}
{"id": "PMID:461145", "title": "[Measurements of serum C3d in primitive chronic glomerular nephropathies (author's transl)].", "content": "Measurement of serum C3 does not provide precise informations concerning an eventual consumption of this complement component during an immunological process. An increased synthetic rate may compensate an accelerated catabolism. The study of breakdown products of C3, such as C3d is a more sensitive approach of the role of complement in some immunological disorders. Therefore C3d was measured in the serum of patients with chronic non systemic glomerular diseases. High values of serum C3d were found in all cases of hypocomplementemic glomerulonephritis. Circulating C3d was also increased to a lower extent, in patients with normocomplementemic nephritis such as minimal change disease, mesangial nephritis with IgA deposits and membraneoproliferative (type I) glomerulonephritis. The data suggested the involvement of complement in a number of glomerulonephritis. Participation of complement in immunological disorders particularly in chronic non systemic glomerulonephritis could require a reevaluation when functional tests are performed in addition to static measurements.", "contents": "[Measurements of serum C3d in primitive chronic glomerular nephropathies (author's transl)]. Measurement of serum C3 does not provide precise informations concerning an eventual consumption of this complement component during an immunological process. An increased synthetic rate may compensate an accelerated catabolism. The study of breakdown products of C3, such as C3d is a more sensitive approach of the role of complement in some immunological disorders. Therefore C3d was measured in the serum of patients with chronic non systemic glomerular diseases. High values of serum C3d were found in all cases of hypocomplementemic glomerulonephritis. Circulating C3d was also increased to a lower extent, in patients with normocomplementemic nephritis such as minimal change disease, mesangial nephritis with IgA deposits and membraneoproliferative (type I) glomerulonephritis. The data suggested the involvement of complement in a number of glomerulonephritis. Participation of complement in immunological disorders particularly in chronic non systemic glomerulonephritis could require a reevaluation when functional tests are performed in addition to static measurements."}
{"id": "PMID:461146", "title": "[Right pneumonectomy for carcinoma with resection of the carena and left tracheo-bronchial anastomosis: 3 cases (author's transl)].", "content": "Extended pneumonectomy for carcinoma is associated with very poor results. However, a tumour which remains limited to the bronchial tree does not perhaps have such a poor prognosis as those which involve neighbouring organs. Valuable survival periods may be obtained after right pneumonectomy with resection of the carena. The authors here report 3 cases, including 2 with survivals of 10 and 8 years respectively.", "contents": "[Right pneumonectomy for carcinoma with resection of the carena and left tracheo-bronchial anastomosis: 3 cases (author's transl)]. Extended pneumonectomy for carcinoma is associated with very poor results. However, a tumour which remains limited to the bronchial tree does not perhaps have such a poor prognosis as those which involve neighbouring organs. Valuable survival periods may be obtained after right pneumonectomy with resection of the carena. The authors here report 3 cases, including 2 with survivals of 10 and 8 years respectively."}
{"id": "PMID:461152", "title": "[Plasma renin and noradrenalin levels during hypotensive therapy using acebutolol (author's transl)].", "content": "The effects of acebutolol (800 mg per day for three days) on pulse rate, blood pressure, plasma noradrenaline and plasma renin activity were measured in nine patients with essential hypertension, at rest in a horizontal position, after five minutes standing upright and after walking for one hour. Acebutolol reduced pulse rate, blood pressure and plasma renin activity in all three positions. Treatment has no influence on plasma noradrenaline in a horizontal position, but significantly reduced its orthostatic stimulation. The inhibition of noradrenergic response to an orthosatic position is compatible with a central or presynaptic effect of acebutolol. This effect would not appear to be essential to its antihypertensive action since acebutolol reduced blood pressure but not plasma noradrenaline in a horizontal position. Blood pressure response to treatment was positively correlated to initial values of plasma renin activity measured after walking for one hour and plasma noradrenaline measured after five minutes in an orthostatic position. On the basis of this correlation, measurement of these two hormonal indices is of predictive value, within the limits of a short period of treatment with acebutolol and strictly defined hospital conditions.", "contents": "[Plasma renin and noradrenalin levels during hypotensive therapy using acebutolol (author's transl)]. The effects of acebutolol (800 mg per day for three days) on pulse rate, blood pressure, plasma noradrenaline and plasma renin activity were measured in nine patients with essential hypertension, at rest in a horizontal position, after five minutes standing upright and after walking for one hour. Acebutolol reduced pulse rate, blood pressure and plasma renin activity in all three positions. Treatment has no influence on plasma noradrenaline in a horizontal position, but significantly reduced its orthostatic stimulation. The inhibition of noradrenergic response to an orthosatic position is compatible with a central or presynaptic effect of acebutolol. This effect would not appear to be essential to its antihypertensive action since acebutolol reduced blood pressure but not plasma noradrenaline in a horizontal position. Blood pressure response to treatment was positively correlated to initial values of plasma renin activity measured after walking for one hour and plasma noradrenaline measured after five minutes in an orthostatic position. On the basis of this correlation, measurement of these two hormonal indices is of predictive value, within the limits of a short period of treatment with acebutolol and strictly defined hospital conditions."}
{"id": "PMID:461153", "title": "[The pharmacokinetics of nalidixic acid administered parenterally. Towards rational use in severe infections (author's transl)].", "content": "Nalidixic acid is effective toward Gram negative infections but its short half-life and potential neurological toxicity explain that its parenteral use is hazardous. Interindividual variations of its pharmacokinetic parameters explain also the absolute necessity of a careful drug monitoring adapted to each patient. The study of plasma kinetics of a single dose of nalidixic acid injected by intravenous route, shows these metabolic variations. With this results, the predicted plasma drug concentrations show that classical dosage regimens are adapted only to a little number of patients. Advantages and inadequacies of a single daily intravenous infusion of nalidixic acid are next analysed.", "contents": "[The pharmacokinetics of nalidixic acid administered parenterally. Towards rational use in severe infections (author's transl)]. Nalidixic acid is effective toward Gram negative infections but its short half-life and potential neurological toxicity explain that its parenteral use is hazardous. Interindividual variations of its pharmacokinetic parameters explain also the absolute necessity of a careful drug monitoring adapted to each patient. The study of plasma kinetics of a single dose of nalidixic acid injected by intravenous route, shows these metabolic variations. With this results, the predicted plasma drug concentrations show that classical dosage regimens are adapted only to a little number of patients. Advantages and inadequacies of a single daily intravenous infusion of nalidixic acid are next analysed."}
{"id": "PMID:461154", "title": "[Defibrination syndrome during acute pancreatitis: 6 cases. Prospective studies of coagulation in 41 patients (author's transl)].", "content": "Studies of coagulation were performed prospectively in 41 patients with mild to moderately severe acute pancreatitis. Six patients (15%) presented with coagulation data suggestive of defibrination; two of them had clinical signs of bleeding. No other cause than pancreatitis was found in these 6 patients to account for coagulation abnormalities. Comparing the patients who presented defibrination to those who did not, no difference was observed in clinical course and admission values of serum amylase, fibrinogen, urea, calcium, glucose, transaminase levels, white blood cell count and arterial partial pressure of oxygen. Platelets counts and serum creatinine levels were respectively lower and higher in the first group of patients.", "contents": "[Defibrination syndrome during acute pancreatitis: 6 cases. Prospective studies of coagulation in 41 patients (author's transl)]. Studies of coagulation were performed prospectively in 41 patients with mild to moderately severe acute pancreatitis. Six patients (15%) presented with coagulation data suggestive of defibrination; two of them had clinical signs of bleeding. No other cause than pancreatitis was found in these 6 patients to account for coagulation abnormalities. Comparing the patients who presented defibrination to those who did not, no difference was observed in clinical course and admission values of serum amylase, fibrinogen, urea, calcium, glucose, transaminase levels, white blood cell count and arterial partial pressure of oxygen. Platelets counts and serum creatinine levels were respectively lower and higher in the first group of patients."}
{"id": "PMID:461155", "title": "[Auto-immune hemolytic anemia associated with Kaposi's sarcoma. Report of a new case (author's transl)].", "content": "The association of Kaposi angiosarcomatosis and an auto-immune haemolytic anaemia is reported in a 69-year-old woman. The direct Coombs test was \"igG complement\" type positive. This is the 5th reported case and the 2nd in which haemolysis developed during an acute malignant exacerbation of the disease. The patient died less than 4 months following the diagnosis of Kaposi's sarcoma, after treatment with prednisone and chlorambucil. Lymphoid and  immunological abnormalities are not rare during the course of Kaposi's sarcoma. The reasons are discussed briefly.", "contents": "[Auto-immune hemolytic anemia associated with Kaposi's sarcoma. Report of a new case (author's transl)]. The association of Kaposi angiosarcomatosis and an auto-immune haemolytic anaemia is reported in a 69-year-old woman. The direct Coombs test was \"igG complement\" type positive. This is the 5th reported case and the 2nd in which haemolysis developed during an acute malignant exacerbation of the disease. The patient died less than 4 months following the diagnosis of Kaposi's sarcoma, after treatment with prednisone and chlorambucil. Lymphoid and  immunological abnormalities are not rare during the course of Kaposi's sarcoma. The reasons are discussed briefly."}
{"id": "PMID:461156", "title": "[Cervical spine. II. Fractures and dislocations (author's transl)].", "content": "Clinical and radiologic investigations in spinal fractures and dislocations must allow assessment of modifications in osteoarticular and neural functions. With respect to osteoarticular function it is necessary to identify the presence of both temporary or permanent instability and impaired static equilibrium. Regarding neural functions we must not only evaluate the neurologic damage but also residual stenosis of the spinal canal and sometimes of the intervertebral foramina. Compression induced by such stenosis is best demonstrated on m\u00e9trizanide myelography. Management is currently aimed at better and more rapid correction of morphologic damages and disturbed stability. These goals are achieved by reduction and stabilisation using orthopedic or surgical techniques, the later involving an anterior or posterior approach to osteosynthesis. Systematic suppression of factors causing neural compression has led to a greater recovery rate than in the past.", "contents": "[Cervical spine. II. Fractures and dislocations (author's transl)]. Clinical and radiologic investigations in spinal fractures and dislocations must allow assessment of modifications in osteoarticular and neural functions. With respect to osteoarticular function it is necessary to identify the presence of both temporary or permanent instability and impaired static equilibrium. Regarding neural functions we must not only evaluate the neurologic damage but also residual stenosis of the spinal canal and sometimes of the intervertebral foramina. Compression induced by such stenosis is best demonstrated on m\u00e9trizanide myelography. Management is currently aimed at better and more rapid correction of morphologic damages and disturbed stability. These goals are achieved by reduction and stabilisation using orthopedic or surgical techniques, the later involving an anterior or posterior approach to osteosynthesis. Systematic suppression of factors causing neural compression has led to a greater recovery rate than in the past."}
{"id": "PMID:461160", "title": "[Blood concentration in glycosylated hemoglobin. Index in diabetic control time (author's transl)].", "content": "To estimate during which period of time the degree of diabetes control is integrated by the level of glycosylated hemoglobin two approachs were used. Hb-g was assayed by cellulose acetate electrophoresis in insulin-dependent diabetics and compared to the degree of control over a 3 months period. Control was evaluated by the semiquantitative urine test for sugar \"Clinitest\" three time a day. Hb-g correlated highly with last month glycosuria, but not as well with those of the two preceding months. These later correlation in fact reflected the stability of control over the three months. In 7 incipient insulin dependent diabetics in whom normal glycaemia was achieved abruptly and maintained for more than one month, Hb-g fell to normal within one month and therefore did not reflect the prexisting hyperglycemia. Hb-g integrates precisely the degree of diabetes control, but over a one month period only.", "contents": "[Blood concentration in glycosylated hemoglobin. Index in diabetic control time (author's transl)]. To estimate during which period of time the degree of diabetes control is integrated by the level of glycosylated hemoglobin two approachs were used. Hb-g was assayed by cellulose acetate electrophoresis in insulin-dependent diabetics and compared to the degree of control over a 3 months period. Control was evaluated by the semiquantitative urine test for sugar \"Clinitest\" three time a day. Hb-g correlated highly with last month glycosuria, but not as well with those of the two preceding months. These later correlation in fact reflected the stability of control over the three months. In 7 incipient insulin dependent diabetics in whom normal glycaemia was achieved abruptly and maintained for more than one month, Hb-g fell to normal within one month and therefore did not reflect the prexisting hyperglycemia. Hb-g integrates precisely the degree of diabetes control, but over a one month period only."}
{"id": "PMID:461161", "title": "[The use of vasopressin during portal bypass (author's transl)].", "content": "Vasopressin was infused intra-arterially or intravenously during 12 portal bypass operations. In comparison with a control group (without vasopressin), there was a very significant reduction in blood loss and portal pressure, and a moderate increase in arterial blood pressure.", "contents": "[The use of vasopressin during portal bypass (author's transl)]. Vasopressin was infused intra-arterially or intravenously during 12 portal bypass operations. In comparison with a control group (without vasopressin), there was a very significant reduction in blood loss and portal pressure, and a moderate increase in arterial blood pressure."}
{"id": "PMID:461162", "title": "[HbA1c: need of its dosage in diabetics (author's transl)].", "content": "HbA1c is the product of a slow, virtually irreversible reaction, occurring continuously throughout the 120 day life-span of the erythrocyte, between HbAo and glucose. It has been shown to represent the time-averaged blood glucose concentration of the preceeding 2 month period, and is independant of day to day fluctuations in the blood glucose concentration. HbA1c therefore acts as a biochemical marker and permits clinicans, by one test, to judge the quality of the diabetic control, in their patients.", "contents": "[HbA1c: need of its dosage in diabetics (author's transl)]. HbA1c is the product of a slow, virtually irreversible reaction, occurring continuously throughout the 120 day life-span of the erythrocyte, between HbAo and glucose. It has been shown to represent the time-averaged blood glucose concentration of the preceeding 2 month period, and is independant of day to day fluctuations in the blood glucose concentration. HbA1c therefore acts as a biochemical marker and permits clinicans, by one test, to judge the quality of the diabetic control, in their patients."}
{"id": "PMID:461173", "title": "[Prolactin secreting adenomas: surgical treatment and results. 120 female cases, 30 males cases (author's transl)].", "content": "Twenty one per cent of the female cases concerned microadenomas discovered because of amenorrhea with or without galactorrhea. Sixty two per cent of these cases were cured by surgery alone, against 40% of the other types of prolactin (Prl) -- secreting tumors with extra-sellar extensions which were not cured. With men, no microadenomas was discovered (although one case demonstrated their possible existence) and in the most frequent cases, tumors exhibited extra-sellar extensions sometimes very large (giant tumors). In these cases, tumoral symptoms were cured by surgery while only 5 out of 30 men recovered a normal plasma Prl. level (30 ng/ml). In all cases the prognosis was established taking into account the volume and invasive characters (at the surgical and histopathological level) of the tumors. The different therapeutical approaches of these tumors (i.e. medical treatment by CB 154, surgery of Rxtherapy) were evaluated. Anyway the necessity of an earlier diagnosis is pointed out allowing the possibility of a selective adenomectomy by a microsurgical approach.", "contents": "[Prolactin secreting adenomas: surgical treatment and results. 120 female cases, 30 males cases (author's transl)]. Twenty one per cent of the female cases concerned microadenomas discovered because of amenorrhea with or without galactorrhea. Sixty two per cent of these cases were cured by surgery alone, against 40% of the other types of prolactin (Prl) -- secreting tumors with extra-sellar extensions which were not cured. With men, no microadenomas was discovered (although one case demonstrated their possible existence) and in the most frequent cases, tumors exhibited extra-sellar extensions sometimes very large (giant tumors). In these cases, tumoral symptoms were cured by surgery while only 5 out of 30 men recovered a normal plasma Prl. level (30 ng/ml). In all cases the prognosis was established taking into account the volume and invasive characters (at the surgical and histopathological level) of the tumors. The different therapeutical approaches of these tumors (i.e. medical treatment by CB 154, surgery of Rxtherapy) were evaluated. Anyway the necessity of an earlier diagnosis is pointed out allowing the possibility of a selective adenomectomy by a microsurgical approach."}
{"id": "PMID:461174", "title": "[Acute agranulocytosis after treatment by levamisole. 2 cases (author's transl)].", "content": "We describe two patients who had a typical levamisole-induced agranulocytosis and a severe infection; the view of other cases found in the literature shows a fundamental fact: the complication can occur after a variable time of prescription (from thirteen days to eleven months), continuous or intermittent. The accidents are imprevisible, and circonspection must be used, not for its antihelminthic properties, but for its prolonged use for immunological purposes, even if some authors have not observed any cases of agranulocytosis in large series. Naturally, the purpose is quite different in the case of a severe rheumatoid arthritis or inesthetic warts, and our two observations are very demonstrative. Mechanism of agranulocytosis has not been totaly clarified: we have observed biological stigmates of immunological process against levamisole (responsible of agranulocytosis?), but essentially medullary lesions (successive regenerative aspects and necrosis) as has been described with pyramidon, and this could explain why the other blood cells are affected in an apparently isolated agranulocytosis.", "contents": "[Acute agranulocytosis after treatment by levamisole. 2 cases (author's transl)]. We describe two patients who had a typical levamisole-induced agranulocytosis and a severe infection; the view of other cases found in the literature shows a fundamental fact: the complication can occur after a variable time of prescription (from thirteen days to eleven months), continuous or intermittent. The accidents are imprevisible, and circonspection must be used, not for its antihelminthic properties, but for its prolonged use for immunological purposes, even if some authors have not observed any cases of agranulocytosis in large series. Naturally, the purpose is quite different in the case of a severe rheumatoid arthritis or inesthetic warts, and our two observations are very demonstrative. Mechanism of agranulocytosis has not been totaly clarified: we have observed biological stigmates of immunological process against levamisole (responsible of agranulocytosis?), but essentially medullary lesions (successive regenerative aspects and necrosis) as has been described with pyramidon, and this could explain why the other blood cells are affected in an apparently isolated agranulocytosis."}
{"id": "PMID:461181", "title": "Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.", "content": "Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polysterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 to 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 mumol per gram of support.", "contents": "Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support. Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polysterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 to 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 mumol per gram of support."}
{"id": "PMID:461182", "title": "DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.", "content": "The specific influence of the four nucleobases on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels under denaturing and nondenaturing conditions has been investigated using homooligomers from the four deoxyribonucleotides as chain length standards. Homooligomers of same chain lengths exhibit remarkable differences in mobility. Specific retardation of any other oligonucleotide investigated was found to be mainly dependent on base composition but not on sequence. A simple procedure is presented for calculating mobilities relative to the standards on denaturing gels. This allows a reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures. The homooligomers also show the same differences in mobility on nondenaturing gels. The significance of this effect for strand separation is discussed.", "contents": "DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels. The specific influence of the four nucleobases on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels under denaturing and nondenaturing conditions has been investigated using homooligomers from the four deoxyribonucleotides as chain length standards. Homooligomers of same chain lengths exhibit remarkable differences in mobility. Specific retardation of any other oligonucleotide investigated was found to be mainly dependent on base composition but not on sequence. A simple procedure is presented for calculating mobilities relative to the standards on denaturing gels. This allows a reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures. The homooligomers also show the same differences in mobility on nondenaturing gels. The significance of this effect for strand separation is discussed."}
{"id": "PMID:461183", "title": "Isolation and organization of calf ribosomal DNA.", "content": "Ribosomal DNA (rDNA) from calf was isolated by three density gradient centrifugations. The first centrifugation in Cs2S04/BAMD was used to obtain partially resolved dG+dC-rich fractions from total DNA. The second and third centrifugations, in Cs2S04/Ag+, led to the isolation of an rDNA fraction characterized by a symmetrical band in CsCl, p = 1.724 g/cm3. This new procedure appears to be generally suitable for the isolation of rDNA and other dG+dC-rich repeated genes. The organization of isolated calf rDNA has been studied by restriction enzyme digestion and by hybridization with cloned rDNA from Xenopus laevis. The repeat unit of calf rDNA has a molecular weight of 21x10(6) and is split by EcoR1 into two fragments, 16x10(6) and 5.0x10(6), and by BamHI into seven fragments. EcoRI and BamHI sites have been mapped. Most of the 18S and 28S RNA genes and the transcribed spacer are contained in the small EcoRI fragment, while the non-transcribed spacer is localized in the large EcoRI fragment. This spacer showed length heterogeneity within a single individual; such heterogeneity is limited to two regions of the spacer.", "contents": "Isolation and organization of calf ribosomal DNA. Ribosomal DNA (rDNA) from calf was isolated by three density gradient centrifugations. The first centrifugation in Cs2S04/BAMD was used to obtain partially resolved dG+dC-rich fractions from total DNA. The second and third centrifugations, in Cs2S04/Ag+, led to the isolation of an rDNA fraction characterized by a symmetrical band in CsCl, p = 1.724 g/cm3. This new procedure appears to be generally suitable for the isolation of rDNA and other dG+dC-rich repeated genes. The organization of isolated calf rDNA has been studied by restriction enzyme digestion and by hybridization with cloned rDNA from Xenopus laevis. The repeat unit of calf rDNA has a molecular weight of 21x10(6) and is split by EcoR1 into two fragments, 16x10(6) and 5.0x10(6), and by BamHI into seven fragments. EcoRI and BamHI sites have been mapped. Most of the 18S and 28S RNA genes and the transcribed spacer are contained in the small EcoRI fragment, while the non-transcribed spacer is localized in the large EcoRI fragment. This spacer showed length heterogeneity within a single individual; such heterogeneity is limited to two regions of the spacer."}
{"id": "PMID:461184", "title": "Anomalous electrophoretic mobility of Drosophila phosphorylated H1 histone: is it related to the compaction of satellite DNA into heterochromatin?", "content": "In embryonic nuclei of Drosophila virilis, 45% of the DNA is satellite, and congruent to 50% of the H1 histone is phosphorylated. In polytene salivary gland nuclei, less than 1% of the DNA is satellite, and less  than  10tion. The phosphorylated H1's migrate 4% slower than the unphosphorylated H1's on SDS-acrylamide gels. The mobility difference may arise because the phosphorylated and unphosphorylated H1's have different conformations in SDS. This putative conformational difference could be essential to the compaction of satellite DNA into heterochromatin.", "contents": "Anomalous electrophoretic mobility of Drosophila phosphorylated H1 histone: is it related to the compaction of satellite DNA into heterochromatin? In embryonic nuclei of Drosophila virilis, 45% of the DNA is satellite, and congruent to 50% of the H1 histone is phosphorylated. In polytene salivary gland nuclei, less than 1% of the DNA is satellite, and less  than  10tion. The phosphorylated H1's migrate 4% slower than the unphosphorylated H1's on SDS-acrylamide gels. The mobility difference may arise because the phosphorylated and unphosphorylated H1's have different conformations in SDS. This putative conformational difference could be essential to the compaction of satellite DNA into heterochromatin."}
{"id": "PMID:461185", "title": "The influence of terminal 3', 5' phosphates on conformations of dApdA.", "content": "Addition of 3' and 5' terminal phosphates to dApdA causes a decrease in conformational flexibility. pdApdAp has much fewer conformers with energies below 2.5 kcal./mole than dApdA. THE A, B and Watson-Crick (34) helices are the most preferred forms. Other important conformations are in the trans domain of psi. Thus, flexibility in psi as well as in omega and omega, and in the sugar pucker is indicated. The transformation from the B helix to the Watson-Crick helix follows a low energy path. This is significant since Watson-Crick conformations may be important for intercalation into nucleic acid polymers (40-42) above the dimer level. The B helix is preferred over the A form in these large DNA subunits.", "contents": "The influence of terminal 3', 5' phosphates on conformations of dApdA. Addition of 3' and 5' terminal phosphates to dApdA causes a decrease in conformational flexibility. pdApdAp has much fewer conformers with energies below 2.5 kcal./mole than dApdA. THE A, B and Watson-Crick (34) helices are the most preferred forms. Other important conformations are in the trans domain of psi. Thus, flexibility in psi as well as in omega and omega, and in the sugar pucker is indicated. The transformation from the B helix to the Watson-Crick helix follows a low energy path. This is significant since Watson-Crick conformations may be important for intercalation into nucleic acid polymers (40-42) above the dimer level. The B helix is preferred over the A form in these large DNA subunits."}
{"id": "PMID:461186", "title": "Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration.", "content": "Nuclear and polysomal polyadenylated RNA populations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10-15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cDNA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysomal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.", "contents": "Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration. Nuclear and polysomal polyadenylated RNA populations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10-15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cDNA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysomal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver."}
{"id": "PMID:461187", "title": "Equilibrium and kinetic aspects of protein-DNA recognition.", "content": "The specificity of regulatory protein binding to DNA is due to a complementarity between the sequence of reaction centres on the protein and the base pair sequence in the specific DNA site allowing the formation of a number of specific noncovalent bonds between the interacting entities. In the present communication the thermodynamic and kinetic aspects of these interactions are considered. The extent of binding specificity is shown to increase with an increase of the bond stability constants and with an increase in the number of ligand reaction centres. Kinetic analysis is carried out assuming that association process is very fast and that dissociation of nonspecific complexes is a rate-limiting step in the recognition of a specific binding site on DNA. The calculations show that a ligand can recognize its specific binding site on DNA within a reasonably limited time interval if the number of its reaction centres and the corresponding stability constants are strongly limited.", "contents": "Equilibrium and kinetic aspects of protein-DNA recognition. The specificity of regulatory protein binding to DNA is due to a complementarity between the sequence of reaction centres on the protein and the base pair sequence in the specific DNA site allowing the formation of a number of specific noncovalent bonds between the interacting entities. In the present communication the thermodynamic and kinetic aspects of these interactions are considered. The extent of binding specificity is shown to increase with an increase of the bond stability constants and with an increase in the number of ligand reaction centres. Kinetic analysis is carried out assuming that association process is very fast and that dissociation of nonspecific complexes is a rate-limiting step in the recognition of a specific binding site on DNA. The calculations show that a ligand can recognize its specific binding site on DNA within a reasonably limited time interval if the number of its reaction centres and the corresponding stability constants are strongly limited."}
{"id": "PMID:461188", "title": "Neighbouring group participation in the unblocking of phosphotriesters of nucleic acids.", "content": "Two examples of neighbouring group participation during the removal of protecting groups from phosphotriesters of partially or fully protected intermediates of nucleic acids are presented. The first example shows that ammonolysis of aryl groups from phosphotriesters of partially protected - 5'- hydroxy free - nucleic acids (e.g., 4b approximately to; Ar=2C1C 6H4) gives rise to the formation of unnatural nucleic acids (e.g., 7 approximately to and 8 approximately to). The second one illustrates that fluoride ion promoted hydrolysis of 2,2,2-trichloroethyl groups from phosphotriesters of fully protected nucleic acids (e.g., 18a approximately to), having t-butyldimethylsilyl groups at the 2'-positions, leads to the formation of a considerable amount of side-products (e.g., 20 approximately to and 21 approximately to).", "contents": "Neighbouring group participation in the unblocking of phosphotriesters of nucleic acids. Two examples of neighbouring group participation during the removal of protecting groups from phosphotriesters of partially or fully protected intermediates of nucleic acids are presented. The first example shows that ammonolysis of aryl groups from phosphotriesters of partially protected - 5'- hydroxy free - nucleic acids (e.g., 4b approximately to; Ar=2C1C 6H4) gives rise to the formation of unnatural nucleic acids (e.g., 7 approximately to and 8 approximately to). The second one illustrates that fluoride ion promoted hydrolysis of 2,2,2-trichloroethyl groups from phosphotriesters of fully protected nucleic acids (e.g., 18a approximately to), having t-butyldimethylsilyl groups at the 2'-positions, leads to the formation of a considerable amount of side-products (e.g., 20 approximately to and 21 approximately to)."}
{"id": "PMID:461189", "title": "Reverse-phase HPLC of DNA restriction fragments and ribooligonucleotides on uncoated Kel-F powder.", "content": "Uncoated Kel-F powder offers some unique features as a support for reverse-phase HPLC of oligonucleotides and DNA restriction fragments. Compounds are eluted from the column by a gradient of acetonitrile (0 tto 18% v/v) in 0.1 M aqueous triethylammonium acetate. In contrast to RPC-5 chromatography, oligonucleotides are not eluted by aqueous salt solutions alone, and the separation of restriction fragments depends only on the chainlength. The packing material is cheap, easy to pack, chemically inert, and does not bleed, so that separations are highly reproducible. The DNA loading capacity for Kel-F is presently inferior to RPC-5, but recovery of microgram amounts of material is typically better than 50%.", "contents": "Reverse-phase HPLC of DNA restriction fragments and ribooligonucleotides on uncoated Kel-F powder. Uncoated Kel-F powder offers some unique features as a support for reverse-phase HPLC of oligonucleotides and DNA restriction fragments. Compounds are eluted from the column by a gradient of acetonitrile (0 tto 18% v/v) in 0.1 M aqueous triethylammonium acetate. In contrast to RPC-5 chromatography, oligonucleotides are not eluted by aqueous salt solutions alone, and the separation of restriction fragments depends only on the chainlength. The packing material is cheap, easy to pack, chemically inert, and does not bleed, so that separations are highly reproducible. The DNA loading capacity for Kel-F is presently inferior to RPC-5, but recovery of microgram amounts of material is typically better than 50%."}
{"id": "PMID:461190", "title": "The 5' terminal capping of heterogeneous nuclear RNA at different embryonic stages of the sea urchin.", "content": "5' Terminal cap structures of hnRNA have been characterized and the extent of capping determined as a function of embryonic development. Sea urchin embryo hnRNA contains only the type-1 cap, m7GpppNmpNp, with the type-2 cap, which has a 2'-0-methylated subpenultimate nucleotide, being associated only with stable small nuclear RNAs. These cap 2-containing RNAs are synthesized at a rate of approximately 70 molecules min-1 nucleus-1 compared to approximately 1000 molecules for hnRNA cap 1. Approximately 70% of nuclear cap 1 is associated with greater than 15S RNA in denaturing solvent, but under non-denaturing conditions the percentage is much higher. Cap 1 in low and high molecular weight nuclear RNA have the same kinetics of methyl labeling. Thus all cap 1 structures may belong to a single class either covalent or H-bonded to high molecular weight RNA. hnRNA greater than 15S is 35% capped; however, adding caps in less than 15S RNA gives an estimate of 50% capping for total hnRNA. In development from early blastula to late gastrula, there is little if any change in the extent of capping of hnRNA. These results coupled with others indicate that the fraction of hnRNA molecules serving as precursor to mRNA does not change quantitatively during embryonic development.", "contents": "The 5' terminal capping of heterogeneous nuclear RNA at different embryonic stages of the sea urchin. 5' Terminal cap structures of hnRNA have been characterized and the extent of capping determined as a function of embryonic development. Sea urchin embryo hnRNA contains only the type-1 cap, m7GpppNmpNp, with the type-2 cap, which has a 2'-0-methylated subpenultimate nucleotide, being associated only with stable small nuclear RNAs. These cap 2-containing RNAs are synthesized at a rate of approximately 70 molecules min-1 nucleus-1 compared to approximately 1000 molecules for hnRNA cap 1. Approximately 70% of nuclear cap 1 is associated with greater than 15S RNA in denaturing solvent, but under non-denaturing conditions the percentage is much higher. Cap 1 in low and high molecular weight nuclear RNA have the same kinetics of methyl labeling. Thus all cap 1 structures may belong to a single class either covalent or H-bonded to high molecular weight RNA. hnRNA greater than 15S is 35% capped; however, adding caps in less than 15S RNA gives an estimate of 50% capping for total hnRNA. In development from early blastula to late gastrula, there is little if any change in the extent of capping of hnRNA. These results coupled with others indicate that the fraction of hnRNA molecules serving as precursor to mRNA does not change quantitatively during embryonic development."}
{"id": "PMID:461191", "title": "1H NMR investigation of the conformational states of DNA in nucleosome core particles.", "content": "In this study 1H NMR has been used to investigate the conformational state of DNA in nucleosome core particles. The nucleosome core particles exhibit partially resolved low field (10-15 ppm) spectra due to imino protons in Watson-Crick base pairs (one resonance per GC or AT base pair). To a first approximation, the spectrum is virtually identical with that of protein-free 140 base pair DNA, and from this observation we draw two important conclusions: (i) Since the low field spectra of DNA are known to be sensitive to conformation, the conformation of DNA in the core particles is essentially the same as that of free DNA (presumably B-form), (ii) since kinks occurring at a frequency at 1 in 10 or 1 in 20 base pairs would result in a core particle spectrum different from that of free DNA we find no NMR evidence supporting either the Crick-Klug or the Sobell models for kinking DNA around the core histones. Linewidth considerations indicate that the rotational correlation time for the core particles is approximately 1.5 X 10(-7) sec, whereas the end-over-end tumbling time of the free 140 base pair DNA is 3 X 10(-7) sec.", "contents": "1H NMR investigation of the conformational states of DNA in nucleosome core particles. In this study 1H NMR has been used to investigate the conformational state of DNA in nucleosome core particles. The nucleosome core particles exhibit partially resolved low field (10-15 ppm) spectra due to imino protons in Watson-Crick base pairs (one resonance per GC or AT base pair). To a first approximation, the spectrum is virtually identical with that of protein-free 140 base pair DNA, and from this observation we draw two important conclusions: (i) Since the low field spectra of DNA are known to be sensitive to conformation, the conformation of DNA in the core particles is essentially the same as that of free DNA (presumably B-form), (ii) since kinks occurring at a frequency at 1 in 10 or 1 in 20 base pairs would result in a core particle spectrum different from that of free DNA we find no NMR evidence supporting either the Crick-Klug or the Sobell models for kinking DNA around the core histones. Linewidth considerations indicate that the rotational correlation time for the core particles is approximately 1.5 X 10(-7) sec, whereas the end-over-end tumbling time of the free 140 base pair DNA is 3 X 10(-7) sec."}
{"id": "PMID:461192", "title": "DNA-binding properties of the major core protein of adenovirus 2.", "content": "The major adenovirus core protein (P.VII) binds to various species of duplex and single-stranded DNA molecules as a linear function of P.VII concentration. P.VII progressively condenses 32S Ad2 DNA into rapidly sedimenting forms having an S value of around 2,280. P.VII does not coat DNA like cytochrome C, instead DNA-protein beads are visualized in the electron microscope at low protein concentration. These beads appear to interact forming larger structures and at high P.VII concentrations the DNA molecule becomes highly compacted. Analysis of DNA fragments formed after digestion of P.VII-DNA complexes and isolated cores with micrococcal nuclease suggest that the organization of the DNA in the two structures is essentially identical. The initial P.VII and DNA interaction is sensitive to both ionic and hydrophobic environments, whereas the in vitro DNA-P.VII complexes are extremely stable and are not disrupted in the presence of 3 M NaCl, 1% sarcosyl or 5% deoxycholate. Properties of these in vitro DNA-protein VII complexes share striking similarities to isolated viral core particles.", "contents": "DNA-binding properties of the major core protein of adenovirus 2. The major adenovirus core protein (P.VII) binds to various species of duplex and single-stranded DNA molecules as a linear function of P.VII concentration. P.VII progressively condenses 32S Ad2 DNA into rapidly sedimenting forms having an S value of around 2,280. P.VII does not coat DNA like cytochrome C, instead DNA-protein beads are visualized in the electron microscope at low protein concentration. These beads appear to interact forming larger structures and at high P.VII concentrations the DNA molecule becomes highly compacted. Analysis of DNA fragments formed after digestion of P.VII-DNA complexes and isolated cores with micrococcal nuclease suggest that the organization of the DNA in the two structures is essentially identical. The initial P.VII and DNA interaction is sensitive to both ionic and hydrophobic environments, whereas the in vitro DNA-P.VII complexes are extremely stable and are not disrupted in the presence of 3 M NaCl, 1% sarcosyl or 5% deoxycholate. Properties of these in vitro DNA-protein VII complexes share striking similarities to isolated viral core particles."}
{"id": "PMID:461193", "title": "Evidence that populations of Dictyostelium single-copy mRNA transcripts carry common repeat sequences.", "content": "Two recombinant plasmids, M4 and KH10, carrying Dictyostelium DNA inserted into the Eco RI restriction endonuclease site of pMB9 by poly(dA)-poly(dT) tailing, were selected for study because they are complementary to abundant mRNA populations from Dictyostelium. Both plasmids have been shown to hybridize a heterogeneous size class of mRNAs which, in the case of KH10, comprise 5-10% of the pulse-labeled poly(A)+ RNA from vegetative cells. Analysis of the sequence organization of the two pieces of Dictyostelium DNA shows that they consist mostly of single-copy sequences with a short DNA sequence which is repeated in the genome and interspersed with single-copy DNA. These and other results suggest that the majority of the hybridization of pulse-labeled mRNA to M4 and KH10 is to the short \"repeated\" DNA sequences. In the genome, members of these repeat families appear to be transcribed onto a population of different single-copy mRNAs. Additional results show that M4 DNA contains a sequence which is entirely complementary to a discrete mRNA.", "contents": "Evidence that populations of Dictyostelium single-copy mRNA transcripts carry common repeat sequences. Two recombinant plasmids, M4 and KH10, carrying Dictyostelium DNA inserted into the Eco RI restriction endonuclease site of pMB9 by poly(dA)-poly(dT) tailing, were selected for study because they are complementary to abundant mRNA populations from Dictyostelium. Both plasmids have been shown to hybridize a heterogeneous size class of mRNAs which, in the case of KH10, comprise 5-10% of the pulse-labeled poly(A)+ RNA from vegetative cells. Analysis of the sequence organization of the two pieces of Dictyostelium DNA shows that they consist mostly of single-copy sequences with a short DNA sequence which is repeated in the genome and interspersed with single-copy DNA. These and other results suggest that the majority of the hybridization of pulse-labeled mRNA to M4 and KH10 is to the short \"repeated\" DNA sequences. In the genome, members of these repeat families appear to be transcribed onto a population of different single-copy mRNAs. Additional results show that M4 DNA contains a sequence which is entirely complementary to a discrete mRNA."}
{"id": "PMID:461194", "title": "Conservation of sequences in related genomes of Apodemus: constraints on the maintenance of satellite DNA sequences.", "content": "Satellites from two related species of the Apodemus genus, A. sylvaticus and A. flavicollis, have been analysed with restriction enzymes Taq I, Alu I and Hind III. The restriction maps are closely conserved between species and show a novel feature of two differing internal periodicities within a 375 base pair repeating unit detected by two different restriction enzymes. This places constraints on the introduction of the observed restriction sites according to current models such as unequal crossing-over. The implications of such a conserved sequence and its presence in other species are discussed.", "contents": "Conservation of sequences in related genomes of Apodemus: constraints on the maintenance of satellite DNA sequences. Satellites from two related species of the Apodemus genus, A. sylvaticus and A. flavicollis, have been analysed with restriction enzymes Taq I, Alu I and Hind III. The restriction maps are closely conserved between species and show a novel feature of two differing internal periodicities within a 375 base pair repeating unit detected by two different restriction enzymes. This places constraints on the introduction of the observed restriction sites according to current models such as unequal crossing-over. The implications of such a conserved sequence and its presence in other species are discussed."}
{"id": "PMID:461195", "title": "Rat growth hormone gene: intervening sequences separate the mRNA regions.", "content": "Rat genomic DNA was digested with various restriction endonucleases, separated by gel electrophoresis and hybridized to 125 I-labeled mRNA for the precursor protein to growth hormone. Restriction sites were found within the genomic DNA that were not found in the previously reported DNA sequence corresponding to the mRNA (19). It appears that the gene for growth hormone contains intervening sequences separating the DNA regions that specify mRNA sequences.", "contents": "Rat growth hormone gene: intervening sequences separate the mRNA regions. Rat genomic DNA was digested with various restriction endonucleases, separated by gel electrophoresis and hybridized to 125 I-labeled mRNA for the precursor protein to growth hormone. Restriction sites were found within the genomic DNA that were not found in the previously reported DNA sequence corresponding to the mRNA (19). It appears that the gene for growth hormone contains intervening sequences separating the DNA regions that specify mRNA sequences."}
{"id": "PMID:461196", "title": "Restriction endonuclease mapping of the human gamma globin gene loci.", "content": "The restriction endonuclease sites in and around the human gamma globin gene loci have been mapped using the gel blotting technique of Southern, in both normal DNA and DNA from an individual with hereditary persistence of fetal hemoglobin (HPFH). In normal DNA, the gamma genes are linked to the delta (and beta) globin genes, and the orientation of these genes with respect to transcription is (5') G gamma leads to A gamma leads to delta leads to beta (3'). The distance between the G gamma and A gamma genes is 3.5 kb and that between the A gamma and delta genes is 16 kb. In both normal DNA and HPFH DNA, the gamma genes are interrupted by an intervening sequence, approximately 1 kb in length that is situated between codon positions 99 and 121 of the coding sequence. In different DNA samples, there is polymorphism for the presence or absence of a Hind III site in the intervening sequence of either gamma golbin gene. In HPFH DNA, a deletion of at least 16 kb of DNA has been detected. This deletion starts at a point approximately 12.5 kb from the 3'-end of A gamma gene and extends through the delta and beta globin genes to a point at least 3 kb beyond the 3'-end of beta globin gene.", "contents": "Restriction endonuclease mapping of the human gamma globin gene loci. The restriction endonuclease sites in and around the human gamma globin gene loci have been mapped using the gel blotting technique of Southern, in both normal DNA and DNA from an individual with hereditary persistence of fetal hemoglobin (HPFH). In normal DNA, the gamma genes are linked to the delta (and beta) globin genes, and the orientation of these genes with respect to transcription is (5') G gamma leads to A gamma leads to delta leads to beta (3'). The distance between the G gamma and A gamma genes is 3.5 kb and that between the A gamma and delta genes is 16 kb. In both normal DNA and HPFH DNA, the gamma genes are interrupted by an intervening sequence, approximately 1 kb in length that is situated between codon positions 99 and 121 of the coding sequence. In different DNA samples, there is polymorphism for the presence or absence of a Hind III site in the intervening sequence of either gamma golbin gene. In HPFH DNA, a deletion of at least 16 kb of DNA has been detected. This deletion starts at a point approximately 12.5 kb from the 3'-end of A gamma gene and extends through the delta and beta globin genes to a point at least 3 kb beyond the 3'-end of beta globin gene."}
{"id": "PMID:461197", "title": "A strategy of DNA sequencing employing computer programs.", "content": "With modern fast sequencing techniques and suitable computer programs it is now possible to sequence whole genomes without the need of restriction maps. This paper describes computer programs that can be used to order both sequence gel readings and clones. A method of coding for uncertainties in gel readings is described. These programs are available on request.", "contents": "A strategy of DNA sequencing employing computer programs. With modern fast sequencing techniques and suitable computer programs it is now possible to sequence whole genomes without the need of restriction maps. This paper describes computer programs that can be used to order both sequence gel readings and clones. A method of coding for uncertainties in gel readings is described. These programs are available on request."}
{"id": "PMID:461198", "title": "Polynucleotides. LVII. Synthesis and properties of poly (2'-chloro-2'-deoxyinosinic acid).", "content": "Poly (2'-chloro-2'-deoxyinosinic acid) [poly(Icl)] was synthesized from Icl 5'-DP by polymerization with polynucleotide phosphorylase. UV absorption properties of poly(Icl) are very similar to those of poly(I). Poly(Icl) adopted a multi-stranded ordered form in the presence of 0.95M Na ion. The Tm value of this form was 36 degrees, which resembles that of poly(I) quadruple-stranded form at high salt. CD spectra also suggested presence of these two forms. Upon mixing with poly(C), poly-(Icl) forms a double-stranded 1 : 1 complex, which had very similar Tm-log[Na+] relationship to that of poly(I) . poly(C). Thus it was concluded that the chlorine substitution at 2'-position of the polynucleotide had the similar effect to OH on physical properties of polynucleotides.", "contents": "Polynucleotides. LVII. Synthesis and properties of poly (2'-chloro-2'-deoxyinosinic acid). Poly (2'-chloro-2'-deoxyinosinic acid) [poly(Icl)] was synthesized from Icl 5'-DP by polymerization with polynucleotide phosphorylase. UV absorption properties of poly(Icl) are very similar to those of poly(I). Poly(Icl) adopted a multi-stranded ordered form in the presence of 0.95M Na ion. The Tm value of this form was 36 degrees, which resembles that of poly(I) quadruple-stranded form at high salt. CD spectra also suggested presence of these two forms. Upon mixing with poly(C), poly-(Icl) forms a double-stranded 1 : 1 complex, which had very similar Tm-log[Na+] relationship to that of poly(I) . poly(C). Thus it was concluded that the chlorine substitution at 2'-position of the polynucleotide had the similar effect to OH on physical properties of polynucleotides."}
{"id": "PMID:461199", "title": "Isolation of the lysozyme gene of chicken.", "content": "The lysozyme gene has been purified by molecular cloning from two chicken gene libraries. Several recombinant phages harbouring sequences homologous to a plasmid carrying a double stranded lysozyme cDNA have been isolated. One recombinant appears to carry an entire lysozyme gene. Electron microscopic studies show that the latter is split by at least three introns. The length of the gene is about 3.9 kb, 6 times longer than lysozyme mRNA.", "contents": "Isolation of the lysozyme gene of chicken. The lysozyme gene has been purified by molecular cloning from two chicken gene libraries. Several recombinant phages harbouring sequences homologous to a plasmid carrying a double stranded lysozyme cDNA have been isolated. One recombinant appears to carry an entire lysozyme gene. Electron microscopic studies show that the latter is split by at least three introns. The length of the gene is about 3.9 kb, 6 times longer than lysozyme mRNA."}
{"id": "PMID:461200", "title": "The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe.", "content": "The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA was determined to be: pG-U-C-A-C-U-A-U-G-U-C-ac4C-G-A-G-D-G-G-D-D-A-A-G-G-A-m2G2-psi-U-A-G-A-N-U-U-C-A-i6A-A-psi-C-U-A-A-U-G-G-G-C-U-U-U-G-C-C-C-G-m5C-G-G-C-A-G-G-T-psi-C-A-m1A-A-U-C-C-U-G-C-U-G-G-U-G-A-C-G-C-C-A OH. The anticodon sequence u ca is complementary to the UGA codon.", "contents": "The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA was determined to be: pG-U-C-A-C-U-A-U-G-U-C-ac4C-G-A-G-D-G-G-D-D-A-A-G-G-A-m2G2-psi-U-A-G-A-N-U-U-C-A-i6A-A-psi-C-U-A-A-U-G-G-G-C-U-U-U-G-C-C-C-G-m5C-G-G-C-A-G-G-T-psi-C-A-m1A-A-U-C-C-U-G-C-U-G-G-U-G-A-C-G-C-C-A OH. The anticodon sequence u ca is complementary to the UGA codon."}
{"id": "PMID:461201", "title": "Cloning of double stranded DNAs derived from polysomal mRNA of maize endosperm: isolation and characterisation of zein clones.", "content": "Starting from polysomal RNA of developing maize endosperm and applying the cloning procedure of integrating dsDNA into the Pst I site of plasmid pBR 322, clones containing sequences complementary to endosperm mRNAs were obtained. 25 per cent of these clones were identified as containing zein specific DNA sequences which hybridized either with the zein mRNA coding for the 22 000 Mr protein or with the zein mRNA coding for the 19 000 Mr protein. The zein-specific DNA inserts of the recombinant plasmids were further characterized by restriction enzyme analysis.", "contents": "Cloning of double stranded DNAs derived from polysomal mRNA of maize endosperm: isolation and characterisation of zein clones. Starting from polysomal RNA of developing maize endosperm and applying the cloning procedure of integrating dsDNA into the Pst I site of plasmid pBR 322, clones containing sequences complementary to endosperm mRNAs were obtained. 25 per cent of these clones were identified as containing zein specific DNA sequences which hybridized either with the zein mRNA coding for the 22 000 Mr protein or with the zein mRNA coding for the 19 000 Mr protein. The zein-specific DNA inserts of the recombinant plasmids were further characterized by restriction enzyme analysis."}
{"id": "PMID:461202", "title": "The chicken conalbumin gene: studies of the organization of cloned DNAs.", "content": "The construction of a double-stranded conalbumin cDNA plasmid (1) has allowed us to investigate the structure of the conalbumin gene. Restriction enzyme mapping of chicken genomic DNA reveals that the conalbumin gene is split and is contained in three EcoRI fragments \"a\", \"b\", \"b\" and \"c\" which have sizes of 10.7, 4 and 2.5 kb, respectively. Analysis with specific probes shows that the orientation of these fragments with respect to transcription is 5'-\"b\", \"c\" and \"a\"-3. The fragments Eco \"b\", Eco \"c\" and part of Eco \"a\" have been isolated by molecular cloning from three different \"libraries\". Electron microscopic studies of hybrids between cloned DNA's and conalbumin mRNA show that one of the isolated clones, lambda C4-conl, contains the coding sequences for the first 940 nucleotides of the mRNA (out of 2400). This region is highly split, since it contains seven short exonic sequences separated by six intervening sequences. The DNA region coding for these 940 nucleotides is 5 times longer than the mRNA coding sequences, a ratio very similar to that found for other chicken genes.", "contents": "The chicken conalbumin gene: studies of the organization of cloned DNAs. The construction of a double-stranded conalbumin cDNA plasmid (1) has allowed us to investigate the structure of the conalbumin gene. Restriction enzyme mapping of chicken genomic DNA reveals that the conalbumin gene is split and is contained in three EcoRI fragments \"a\", \"b\", \"b\" and \"c\" which have sizes of 10.7, 4 and 2.5 kb, respectively. Analysis with specific probes shows that the orientation of these fragments with respect to transcription is 5'-\"b\", \"c\" and \"a\"-3. The fragments Eco \"b\", Eco \"c\" and part of Eco \"a\" have been isolated by molecular cloning from three different \"libraries\". Electron microscopic studies of hybrids between cloned DNA's and conalbumin mRNA show that one of the isolated clones, lambda C4-conl, contains the coding sequences for the first 940 nucleotides of the mRNA (out of 2400). This region is highly split, since it contains seven short exonic sequences separated by six intervening sequences. The DNA region coding for these 940 nucleotides is 5 times longer than the mRNA coding sequences, a ratio very similar to that found for other chicken genes."}
{"id": "PMID:461203", "title": "The structure of the human beta-globin gene in beta-thalassaemia.", "content": "Twenty-one cases of beta 0 and beta +-thalassaemia have been analysed by restriction endonuclease mapping. In most cases no deletion in the regions surrounding the beta- and delta-globin genes could be detected. However, in a single Asian case of beta 0-thalassaemia, homozygous clinically, one of the homologous chromosomes contained a beta-globin gene with a deletion of 600 base pairs of DNA and comprising most or all of the 3' end of the structural gene including the EcoRI restriction site within the beta-globin coding sequence.", "contents": "The structure of the human beta-globin gene in beta-thalassaemia. Twenty-one cases of beta 0 and beta +-thalassaemia have been analysed by restriction endonuclease mapping. In most cases no deletion in the regions surrounding the beta- and delta-globin genes could be detected. However, in a single Asian case of beta 0-thalassaemia, homozygous clinically, one of the homologous chromosomes contained a beta-globin gene with a deletion of 600 base pairs of DNA and comprising most or all of the 3' end of the structural gene including the EcoRI restriction site within the beta-globin coding sequence."}
{"id": "PMID:461204", "title": "Heterogeneity of the chromosome fiber.", "content": "Evidence is presented that the 250 A thick chromosome fiber consists of clusters of nucleosomes (superbeads) which are heterogeneous in size. In bovine lymphocyte chromatin their number average corresponds to about 12 nucleosomes.", "contents": "Heterogeneity of the chromosome fiber. Evidence is presented that the 250 A thick chromosome fiber consists of clusters of nucleosomes (superbeads) which are heterogeneous in size. In bovine lymphocyte chromatin their number average corresponds to about 12 nucleosomes."}
{"id": "PMID:461205", "title": "Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells.", "content": "Micrococcal-nuclease digestion of adenovirus 2(ad 2) infected HeLa cell nuclei early after infection has been used to investigate the nucleoprotein nature of parental viral DNA. Viral DNA is more susceptible to nuclease digestion than cellular DNA. The pattern of digestion products changes as digestion proceeds from an indistinct pattern 1 hour post infection(pi) to a nucleosome-like pattern at 6 hours pi. The major differences between viral and cellular nucleoprotein products were i) a subnucleosome fraction from viral DNA and ii) the repeat size of DNA in viral nucleosomes was 165 base pairs and in cellular nucleosomes, 195 base pairs. Up to 50% viral DNA in nuclei 6 hours pi seems to be in nucleosome-like structures. Such patterns are not seen on digestion of partially-uncoated virus or isolated cores.", "contents": "Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells. Micrococcal-nuclease digestion of adenovirus 2(ad 2) infected HeLa cell nuclei early after infection has been used to investigate the nucleoprotein nature of parental viral DNA. Viral DNA is more susceptible to nuclease digestion than cellular DNA. The pattern of digestion products changes as digestion proceeds from an indistinct pattern 1 hour post infection(pi) to a nucleosome-like pattern at 6 hours pi. The major differences between viral and cellular nucleoprotein products were i) a subnucleosome fraction from viral DNA and ii) the repeat size of DNA in viral nucleosomes was 165 base pairs and in cellular nucleosomes, 195 base pairs. Up to 50% viral DNA in nuclei 6 hours pi seems to be in nucleosome-like structures. Such patterns are not seen on digestion of partially-uncoated virus or isolated cores."}
{"id": "PMID:461206", "title": "Influence of nucleotide sequence on dA.dT-specific binding of Netropsin to double stranded DNA.", "content": "Using CD measurements the complex formation of Netropsin (Nt) with poly(dA-dC).poly(dT-dG) and its stability against high salt concentrations is compared with that of poly(dA).poly(dT) and poly(dA-dT).POLY(DT-dA). It is experimentally shown that the insertion of a dG.dC pair in dA.dT sequences strongly reduces the specific interaction of Nt with DNA duplexes. The specificity of the interaction is strongly increased by two or more consecutive thymine residues as present in thymine isostichs of double stranded DNA's.", "contents": "Influence of nucleotide sequence on dA.dT-specific binding of Netropsin to double stranded DNA. Using CD measurements the complex formation of Netropsin (Nt) with poly(dA-dC).poly(dT-dG) and its stability against high salt concentrations is compared with that of poly(dA).poly(dT) and poly(dA-dT).POLY(DT-dA). It is experimentally shown that the insertion of a dG.dC pair in dA.dT sequences strongly reduces the specific interaction of Nt with DNA duplexes. The specificity of the interaction is strongly increased by two or more consecutive thymine residues as present in thymine isostichs of double stranded DNA's."}
{"id": "PMID:461207", "title": "Effect of 5-alkyl substitution of uracil on the thermal stability of poly [d(A-r5U)] copolymers.", "content": "The thermal transition of poly[d(A-r5U)] polydeoxynucleotides (where r was a hydrogen atom, or a methyl, ethyl, n-propyl, n-butyl or n-pentyl group) was studied by measuring the derivative melting profiles of the polymers in the range of 0.01--0.36 M K+, at pH 6.8. According to the Tm values, polydeoxynucleotide analogues show lower thermal stability than poly[d(A-T)] at any counterion concentration applied. At a given salt concentration, Tm of the alkyl analogues decreased as the number of carbon atoms (n) in the r substituent of poly[d(A-r5U)] increased. 1/Tm plotted against against 1/n yielded a linear relationship. Cooperativity of the melting of all poly[d(A--U)] analogues decreased with the increase of salt concentration in the solution. This change depended again on 5-substitution of the uracil moiety of poly[d(A-U)]. Smallest decrease was observed in the case of poly[d(A--U)] whereas largest decrease was shown by poly[d(A-pe5U)] (pe=pentyl group).", "contents": "Effect of 5-alkyl substitution of uracil on the thermal stability of poly [d(A-r5U)] copolymers. The thermal transition of poly[d(A-r5U)] polydeoxynucleotides (where r was a hydrogen atom, or a methyl, ethyl, n-propyl, n-butyl or n-pentyl group) was studied by measuring the derivative melting profiles of the polymers in the range of 0.01--0.36 M K+, at pH 6.8. According to the Tm values, polydeoxynucleotide analogues show lower thermal stability than poly[d(A-T)] at any counterion concentration applied. At a given salt concentration, Tm of the alkyl analogues decreased as the number of carbon atoms (n) in the r substituent of poly[d(A-r5U)] increased. 1/Tm plotted against against 1/n yielded a linear relationship. Cooperativity of the melting of all poly[d(A--U)] analogues decreased with the increase of salt concentration in the solution. This change depended again on 5-substitution of the uracil moiety of poly[d(A-U)]. Smallest decrease was observed in the case of poly[d(A--U)] whereas largest decrease was shown by poly[d(A-pe5U)] (pe=pentyl group)."}
{"id": "PMID:461208", "title": "Cations and the accessibility of chromatin to nucleases.", "content": "When rat liver nuclei prepared with polyamines as stabilising cations are digested with DNAase II, release of both inactive chromatin and Mg-soluble, active chromatin is greatly reduced, in comparison to digestion of liver nuclei prepared with Mg2+ as stabilising cation. Chromatin release from polyamine stabilised nuclei is also inhibited relative to Mg-stabilised nuclei following digestion with micrococcal nuclease under two very different cation conditions. Nuclei prepared with polyamines and monovalent ions as stabilising cations exhibit properties intermediate between these two extremes with both nucleases. These effects are due to residual binding of polyamines to chromatin, which is thus maintained in a condensed state, inaccessible to nucleases. Since polyamine binding is not easily reversed, concentrations of polyamines and other cations must be rigidly controlled in experiments on chromatin structure if artefacts are to be avoided. The significance of these findings to the nature and properties of active chromatin within the intact nucleus is considered.", "contents": "Cations and the accessibility of chromatin to nucleases. When rat liver nuclei prepared with polyamines as stabilising cations are digested with DNAase II, release of both inactive chromatin and Mg-soluble, active chromatin is greatly reduced, in comparison to digestion of liver nuclei prepared with Mg2+ as stabilising cation. Chromatin release from polyamine stabilised nuclei is also inhibited relative to Mg-stabilised nuclei following digestion with micrococcal nuclease under two very different cation conditions. Nuclei prepared with polyamines and monovalent ions as stabilising cations exhibit properties intermediate between these two extremes with both nucleases. These effects are due to residual binding of polyamines to chromatin, which is thus maintained in a condensed state, inaccessible to nucleases. Since polyamine binding is not easily reversed, concentrations of polyamines and other cations must be rigidly controlled in experiments on chromatin structure if artefacts are to be avoided. The significance of these findings to the nature and properties of active chromatin within the intact nucleus is considered."}
{"id": "PMID:461215", "title": "The value of nerve biopsies.", "content": "A short review on the usefullness of nerve biopsies is given. After a short description of the features of axonal degeneration and demyelination the possibility of diagnosing neurolipidoses from nerve biopsies is stressed.", "contents": "The value of nerve biopsies. A short review on the usefullness of nerve biopsies is given. After a short description of the features of axonal degeneration and demyelination the possibility of diagnosing neurolipidoses from nerve biopsies is stressed."}
{"id": "PMID:461216", "title": "Some remarks on assessing morphologic changes in old, demented people.", "content": "Some aspects of quantitative assessment of senile and vascular changes in brains of old, demented people are discussed. Particular emphasis is placed upon neuritic plaque count and upon the amount of softened brain parenchyma relative to the degree of intellectual deterioration. The functional significance of the topography of ischemic lesions is stressed in some cases.", "contents": "Some remarks on assessing morphologic changes in old, demented people. Some aspects of quantitative assessment of senile and vascular changes in brains of old, demented people are discussed. Particular emphasis is placed upon neuritic plaque count and upon the amount of softened brain parenchyma relative to the degree of intellectual deterioration. The functional significance of the topography of ischemic lesions is stressed in some cases."}
{"id": "PMID:461221", "title": "Morphologic findings in central bronchi correlated to lung function data in obstructive airways disease.", "content": "Quantitative analyses of morphologic findings are the condition of clinico-pathological correlation studies. These quantitative analyses are possible by morphometry. The present correlation study shows that morphometry may therefore contribute to understanding the pathogenesis of obstructive airways disease: decreasing bronchial lumen and increasing volume of grandular ducts are correlated to increasing airways resistances (clinical parameters: RAW, FEV1). Increasing volume of bronchial muscles is correlated with resistance of airways at quiet breathing (RAW) and less with increasing residual volume (RV). Increasing volume of bronchial glands and glandular ducts is correlated with increasing resistance at forced expiration which is clinically shown by decreasing FEV1. These findings can be interpreted as follows. Airway resistances are mainly influenced by airway narrowing. At quiet breathing, muscle constriction is an additional cause of increasing airflow resistance due to bronchial narrowing. At forced expiration, however, mucus plugs probably limit the airflow because thickening of bronchial glands points to increased secretory activity. Until now it is not possible to understand why bronchial muscle volume correlates with residual volume.", "contents": "Morphologic findings in central bronchi correlated to lung function data in obstructive airways disease. Quantitative analyses of morphologic findings are the condition of clinico-pathological correlation studies. These quantitative analyses are possible by morphometry. The present correlation study shows that morphometry may therefore contribute to understanding the pathogenesis of obstructive airways disease: decreasing bronchial lumen and increasing volume of grandular ducts are correlated to increasing airways resistances (clinical parameters: RAW, FEV1). Increasing volume of bronchial muscles is correlated with resistance of airways at quiet breathing (RAW) and less with increasing residual volume (RV). Increasing volume of bronchial glands and glandular ducts is correlated with increasing resistance at forced expiration which is clinically shown by decreasing FEV1. These findings can be interpreted as follows. Airway resistances are mainly influenced by airway narrowing. At quiet breathing, muscle constriction is an additional cause of increasing airflow resistance due to bronchial narrowing. At forced expiration, however, mucus plugs probably limit the airflow because thickening of bronchial glands points to increased secretory activity. Until now it is not possible to understand why bronchial muscle volume correlates with residual volume."}
{"id": "PMID:461222", "title": "The morphologic diagnosis of analgesic (phenacetin) abuse.", "content": "A number of different morphologic characteristics were examined to determine their relative values in establishing a purely morphologic diagnosis of phenacetin abuse. These included hyperpigmentation of the skin, the costal cartilages, the liver, and the renal tubules, and capillarosclerosis of the lower urinary tract. Hyperpigmentation of the skin, liver, and renal tubules cannot be used in the diagnosis of phenacetin abuse. Massive brown pigmentation of the costal cartilages in patients under 60 years of age suggests phenacetin abuse, but even this morphologic parameter, when used alone, is insufficient to establish a definite diagnosis. Capillarosclerosis in the lower urinary tract does, however, permit one to diagnose phenacetin abuse with certainty, as it is found exclusively in this condition.", "contents": "The morphologic diagnosis of analgesic (phenacetin) abuse. A number of different morphologic characteristics were examined to determine their relative values in establishing a purely morphologic diagnosis of phenacetin abuse. These included hyperpigmentation of the skin, the costal cartilages, the liver, and the renal tubules, and capillarosclerosis of the lower urinary tract. Hyperpigmentation of the skin, liver, and renal tubules cannot be used in the diagnosis of phenacetin abuse. Massive brown pigmentation of the costal cartilages in patients under 60 years of age suggests phenacetin abuse, but even this morphologic parameter, when used alone, is insufficient to establish a definite diagnosis. Capillarosclerosis in the lower urinary tract does, however, permit one to diagnose phenacetin abuse with certainty, as it is found exclusively in this condition."}
{"id": "PMID:461223", "title": "[Diagnostic progress in familial nephropathy. Alport's syndrome, nail-patella syndrome and benign familial hematuria (author's transl)].", "content": "Progress in the field of morphology and new insights in human genetics, have led to the early diagnosis of hereditary nephropathy in growing number of cases. Light microscopic findings are not specific. Using electron microscopy, however, cases with primary lesions of the peripheral glomerular basement membrane (Alport's syndrome, benign familial hematuria, nail-patella syndrome) exhibit pathognomonic morphologic changes even in early stages of the disease. Electron microscopy is thus the only effective diagnostic method in suspicious cases.", "contents": "[Diagnostic progress in familial nephropathy. Alport's syndrome, nail-patella syndrome and benign familial hematuria (author's transl)]. Progress in the field of morphology and new insights in human genetics, have led to the early diagnosis of hereditary nephropathy in growing number of cases. Light microscopic findings are not specific. Using electron microscopy, however, cases with primary lesions of the peripheral glomerular basement membrane (Alport's syndrome, benign familial hematuria, nail-patella syndrome) exhibit pathognomonic morphologic changes even in early stages of the disease. Electron microscopy is thus the only effective diagnostic method in suspicious cases."}
{"id": "PMID:461224", "title": "Lung biopsy: methods, value, complications, timing, and indications.", "content": "Six methods of lung biopsy are discussed on the basis of literature reports: 1. Aspiration biopsy by fine needle, 2. split needle biopsy (Vim-Silverman), 3. cutting needle biopsy (Travenol), 4. trephine biopsy by rotating needle, 5. transbronchial biopsy, 6. open biopsy. Because of the high risk, biopsies with thick needles (methods 2--4) should be abandoned. Transbronchial biopsy is renounced because reliable results are only achieved in sarcoidosis which can be clearly diagnosed in high percentage of cases by other less dangerous procedures. In solitary tumors which could not be diagnosed by any other means aspiration biopsy is indicated because of its low risk and its diagnostic accuracy of more than 80%. In disseminated pulmonary disorders which cannot be diagnosed by any other means open lung biopsy is the most reliable method. The lung specimens can be taken under eye control. They should contain tissue from the transitional zone between diseased and unchanged areas, however. The specimens are usually big enough for additional electron and fluorescence microscopic examinations as well as dust analyses. The pathologist must be aware of all clinical data including X-ray pictures, laboratory findings, case history, and history of occupational and nonoccupational dust exposure. If these conditions are fulfilled and adequate methods are applied, lung biopsy provides diagnosis and prognosis, makes causal therapy possible, and amplifies the medical knowledge of pulmonary diseases.", "contents": "Lung biopsy: methods, value, complications, timing, and indications. Six methods of lung biopsy are discussed on the basis of literature reports: 1. Aspiration biopsy by fine needle, 2. split needle biopsy (Vim-Silverman), 3. cutting needle biopsy (Travenol), 4. trephine biopsy by rotating needle, 5. transbronchial biopsy, 6. open biopsy. Because of the high risk, biopsies with thick needles (methods 2--4) should be abandoned. Transbronchial biopsy is renounced because reliable results are only achieved in sarcoidosis which can be clearly diagnosed in high percentage of cases by other less dangerous procedures. In solitary tumors which could not be diagnosed by any other means aspiration biopsy is indicated because of its low risk and its diagnostic accuracy of more than 80%. In disseminated pulmonary disorders which cannot be diagnosed by any other means open lung biopsy is the most reliable method. The lung specimens can be taken under eye control. They should contain tissue from the transitional zone between diseased and unchanged areas, however. The specimens are usually big enough for additional electron and fluorescence microscopic examinations as well as dust analyses. The pathologist must be aware of all clinical data including X-ray pictures, laboratory findings, case history, and history of occupational and nonoccupational dust exposure. If these conditions are fulfilled and adequate methods are applied, lung biopsy provides diagnosis and prognosis, makes causal therapy possible, and amplifies the medical knowledge of pulmonary diseases."}
{"id": "PMID:461225", "title": "Effect of cortisone and X-irradiation on cellular depletion and regeneration in the thymus of mice: experimental discrimination between thymus lymphocyte precursors in the bone marrow and in the thymus.", "content": "The effect of cortisone treatment on the ability of bone marrow cells to repopulate X-irradiated thymus was investigated. In one experimental series, groups of mice were treated first with cortisone and then irradiated with or without bone marrow protection. Mice treated with either cortisone or radiation alone served as controls. During an initial, bone marrow independent phase of thymus regeneration, cortisone had a stronger inhibitory effect on the cellular regeneration of the organ than irradiation. On the other hand, during a subsequent bone marrow dependent phase, thymus regeneration was impaired by radiation exposure but not by cortisone treatment. In another experimental series, irradiated mice were transplanted with bone marrow cells in different numbers from syngeneic donors which had either been treated with cortisone or were left untreated. Twenty days later the cell number was consistently larger in the thymus of animals which has been transplanted with cortisone treated bone marrow than in the animals transplanted with untreated bone marrow. It is concluded that the thymus lymphocyte precursors in the bone marrow and the early precursors of thymocytes in the thymus differ with regard to their sensitivity to cortisone and radiation and, therefore, may represent two distinct cell types.", "contents": "Effect of cortisone and X-irradiation on cellular depletion and regeneration in the thymus of mice: experimental discrimination between thymus lymphocyte precursors in the bone marrow and in the thymus. The effect of cortisone treatment on the ability of bone marrow cells to repopulate X-irradiated thymus was investigated. In one experimental series, groups of mice were treated first with cortisone and then irradiated with or without bone marrow protection. Mice treated with either cortisone or radiation alone served as controls. During an initial, bone marrow independent phase of thymus regeneration, cortisone had a stronger inhibitory effect on the cellular regeneration of the organ than irradiation. On the other hand, during a subsequent bone marrow dependent phase, thymus regeneration was impaired by radiation exposure but not by cortisone treatment. In another experimental series, irradiated mice were transplanted with bone marrow cells in different numbers from syngeneic donors which had either been treated with cortisone or were left untreated. Twenty days later the cell number was consistently larger in the thymus of animals which has been transplanted with cortisone treated bone marrow than in the animals transplanted with untreated bone marrow. It is concluded that the thymus lymphocyte precursors in the bone marrow and the early precursors of thymocytes in the thymus differ with regard to their sensitivity to cortisone and radiation and, therefore, may represent two distinct cell types."}
{"id": "PMID:461226", "title": "Morphologic and cell kinetic investigations of the spleen after repeated in situ freezing of liver and kidney.", "content": "In order to evaluate a possible primary or secondary immunologic response of the spleen after single or repeated in situ freezing of parenchymal organs such as liver and kidney within a four week period, light microscopic and cell kinetic investigations with tritiated thymidine were performed on spleens of non-germfree rats. Sham operations served as controls. The sham operations did not induce any significant morphological or cell kinetic changes in the splenic white pulp. After cryolesions were produced in the liver and kidney, the percentages of activated germinal centers, labeled germinal center cells, and cells in the perifollicular area of the lymphatic mantle and marginal zones increased, with maxima during the first 3 days. The investigations show that the cellular reaction of the spleen starts earlier and is more prominent after repeated in situ freezing than after a single cryolesion. These findings point to an immunologic response of the anamnestic type, and correspond to results after repeated freezing of normal and malignant tissue of the urogenital tract. These cell kinetic results are important in the evaluation of further immunologic studies involving the cryotherapy of malignant tissues.", "contents": "Morphologic and cell kinetic investigations of the spleen after repeated in situ freezing of liver and kidney. In order to evaluate a possible primary or secondary immunologic response of the spleen after single or repeated in situ freezing of parenchymal organs such as liver and kidney within a four week period, light microscopic and cell kinetic investigations with tritiated thymidine were performed on spleens of non-germfree rats. Sham operations served as controls. The sham operations did not induce any significant morphological or cell kinetic changes in the splenic white pulp. After cryolesions were produced in the liver and kidney, the percentages of activated germinal centers, labeled germinal center cells, and cells in the perifollicular area of the lymphatic mantle and marginal zones increased, with maxima during the first 3 days. The investigations show that the cellular reaction of the spleen starts earlier and is more prominent after repeated in situ freezing than after a single cryolesion. These findings point to an immunologic response of the anamnestic type, and correspond to results after repeated freezing of normal and malignant tissue of the urogenital tract. These cell kinetic results are important in the evaluation of further immunologic studies involving the cryotherapy of malignant tissues."}
{"id": "PMID:461227", "title": "Iron negative foci and nodules in safrole-exposed mouse liver made siderotic by iron-dextran injection.", "content": "A procedure for the production of mouse hepatic siderosis is described which results in extensive iron deposition in all lobular zones. Mice exposed to safrole for 24 weeks displayed basophilic and acidophilic foci which did not accumulate iron. 36 weeks of dietary safrole exposure resulted in nodular lesions comprised of basophilic and hyalinized cells. The nodules displayed decreased or negative reactions for iron in hepatic parenchymal cells when the surrounding liver was siderotic.", "contents": "Iron negative foci and nodules in safrole-exposed mouse liver made siderotic by iron-dextran injection. A procedure for the production of mouse hepatic siderosis is described which results in extensive iron deposition in all lobular zones. Mice exposed to safrole for 24 weeks displayed basophilic and acidophilic foci which did not accumulate iron. 36 weeks of dietary safrole exposure resulted in nodular lesions comprised of basophilic and hyalinized cells. The nodules displayed decreased or negative reactions for iron in hepatic parenchymal cells when the surrounding liver was siderotic."}
{"id": "PMID:461228", "title": "Chronic anterior tibial compartment syndrome. A light-microscopic, histochemical and electronmicroscopic analysis.", "content": "A case of chronic anterior tibial compartment syndrome is reported where the entity was accompanied with a primary neurogenic type of muscle atrophy. Histology, histochemistry and electronmicroscopy indicated the diagnosis. The authors concluded that in a moderate case of leg trauma with peroneal nerve involvement, when it is complicated by intermittent arterial spasm, the anterior tibial compartment syndrome may develop chronically.", "contents": "Chronic anterior tibial compartment syndrome. A light-microscopic, histochemical and electronmicroscopic analysis. A case of chronic anterior tibial compartment syndrome is reported where the entity was accompanied with a primary neurogenic type of muscle atrophy. Histology, histochemistry and electronmicroscopy indicated the diagnosis. The authors concluded that in a moderate case of leg trauma with peroneal nerve involvement, when it is complicated by intermittent arterial spasm, the anterior tibial compartment syndrome may develop chronically."}
{"id": "PMID:461230", "title": "Tritiated thymidine autoradiographic study on histogenesis and spreading of intestinal metaplasia in human stomach.", "content": "The non-diseased portions of the antral mucosa of patients suffering from gastric cancer or ulcer were biopsied. The biopsy specimens were then labelled with 3H-thymidine in vitro, and distribution of the labelled epithelial cells in the normal pyloric and in the intestinalized mucosa was studied with autoradiography, and modes of histogenesis and spreading of the intestinal metaplasia were studied, and kinetic characteristics of the intestinalized mucosa were discussed. In the normal pyloric mucosa, the labelled cells were confined to the isthmus region (the middle one-third level of the mucosa), indicating that the surface epithelial and the pyloric glandular cells are normally replaced from the isthmus region. On the other hand, a zone of the labelled cells was found at the lower one-third level in the intestinalized mucosa. The absorptive and the goblet cells in the intestinalized mucosa appear to be renewed by about 70 hours in a fashion similar to that of the small intestine. Microscopic and autoradiographic analysis of the antral mucosa in the course of intestinalization indicates that the intestinal metaplasia begins in the isthmus region of the pyloric glandular tubules of an intact mucosa unaffected by gross injury through transformation of the generative cells from a pyloric to an intestinal pattern. This permits the pyloric lining cells to be replaced with intestinal villous cells and also permits the generative cell zone of the intestinal tubules to shift from the isthmus to the base of the gland until the process is complete. The downward shift of the intestinal tubules occurs in a framework of one of the branched pyloric glands and other glands disappear, resulting in a change of mucosal architectures of the antrum from a branched to a simple tubular gland. The intestinal metaplasia spreads in the mucosa through multi-focal (and sporadical) transformation of the neck generative cells in individual glandular tubules.", "contents": "Tritiated thymidine autoradiographic study on histogenesis and spreading of intestinal metaplasia in human stomach. The non-diseased portions of the antral mucosa of patients suffering from gastric cancer or ulcer were biopsied. The biopsy specimens were then labelled with 3H-thymidine in vitro, and distribution of the labelled epithelial cells in the normal pyloric and in the intestinalized mucosa was studied with autoradiography, and modes of histogenesis and spreading of the intestinal metaplasia were studied, and kinetic characteristics of the intestinalized mucosa were discussed. In the normal pyloric mucosa, the labelled cells were confined to the isthmus region (the middle one-third level of the mucosa), indicating that the surface epithelial and the pyloric glandular cells are normally replaced from the isthmus region. On the other hand, a zone of the labelled cells was found at the lower one-third level in the intestinalized mucosa. The absorptive and the goblet cells in the intestinalized mucosa appear to be renewed by about 70 hours in a fashion similar to that of the small intestine. Microscopic and autoradiographic analysis of the antral mucosa in the course of intestinalization indicates that the intestinal metaplasia begins in the isthmus region of the pyloric glandular tubules of an intact mucosa unaffected by gross injury through transformation of the generative cells from a pyloric to an intestinal pattern. This permits the pyloric lining cells to be replaced with intestinal villous cells and also permits the generative cell zone of the intestinal tubules to shift from the isthmus to the base of the gland until the process is complete. The downward shift of the intestinal tubules occurs in a framework of one of the branched pyloric glands and other glands disappear, resulting in a change of mucosal architectures of the antrum from a branched to a simple tubular gland. The intestinal metaplasia spreads in the mucosa through multi-focal (and sporadical) transformation of the neck generative cells in individual glandular tubules."}
{"id": "PMID:461231", "title": "Relation of adenomatous hyperplasia of the gastric mucosa to carcinogenesis.", "content": "Experimental studies of stomach carcinogenesis were carried out in two series of Wistar rats (66 and 174 animals). In both series submucosal foci of adenomatous hyperplasia were observed either without atypia, or with atypia at different, gradually increasing degrees. The number of these focal lesions in the submucosa (87 in the first series) was smaller than that of focal dysplastic changes with varying grades  of atypia within the mucosa. On the basis of different degrees of atypia observed in these adenomatous hyperplasias, we have to assume that a phase shifting occurs in those areas that show lower grades or absence of atypia. In the framework of carcinogenesis this kind of adenomatous hyperplasia is interpreted as an incomplete or incompletely persisting carcinogenesis. In analogy to our experimental findings we found identical lesions in three human cases where different grades of atypia were observed distant from the primary stomach cancer. These results are discussed with reference to the animal experiments and to the literature.", "contents": "Relation of adenomatous hyperplasia of the gastric mucosa to carcinogenesis. Experimental studies of stomach carcinogenesis were carried out in two series of Wistar rats (66 and 174 animals). In both series submucosal foci of adenomatous hyperplasia were observed either without atypia, or with atypia at different, gradually increasing degrees. The number of these focal lesions in the submucosa (87 in the first series) was smaller than that of focal dysplastic changes with varying grades  of atypia within the mucosa. On the basis of different degrees of atypia observed in these adenomatous hyperplasias, we have to assume that a phase shifting occurs in those areas that show lower grades or absence of atypia. In the framework of carcinogenesis this kind of adenomatous hyperplasia is interpreted as an incomplete or incompletely persisting carcinogenesis. In analogy to our experimental findings we found identical lesions in three human cases where different grades of atypia were observed distant from the primary stomach cancer. These results are discussed with reference to the animal experiments and to the literature."}
{"id": "PMID:461232", "title": "Loss of differentiation in intestinal metaplasia in cancerous stomachs. A comparative morphologic study.", "content": "229 stomachs resected for duodenal and gastric ulcer and carcinoma were examined with special regard to the morphological and histochemical pattern of intestinal metaplasia (IM). The results of qualitative and semiquantitative studies were analysed statistically. Whereas duodenal and gastric ulcer cases are best discriminated by the presence or absence of IM, the strongest discriminating factor between carcinoma and gastric ulcer is the content of goblet cells in metaplastic crypts. Metaplastic crypts lined exclusively with goblet cells producing sulfated acid glycoproteins could be identified in more than one third of the cancer cases. The increase in goblet cells coincides with a loss of the more differentiated cells in the metaplastic glands, such as enterocytes, APUD cells, or Paneth cells. This \"enterocoli metaplasia\" seems to be specific for cancer bearing mucosa and occurs more often in cancer of intestinal type; it may represent a form of a derepressive dedifferentiation. The significance of enterocoli metaplasia as a premalignant lesion remains to be elucidated.", "contents": "Loss of differentiation in intestinal metaplasia in cancerous stomachs. A comparative morphologic study. 229 stomachs resected for duodenal and gastric ulcer and carcinoma were examined with special regard to the morphological and histochemical pattern of intestinal metaplasia (IM). The results of qualitative and semiquantitative studies were analysed statistically. Whereas duodenal and gastric ulcer cases are best discriminated by the presence or absence of IM, the strongest discriminating factor between carcinoma and gastric ulcer is the content of goblet cells in metaplastic crypts. Metaplastic crypts lined exclusively with goblet cells producing sulfated acid glycoproteins could be identified in more than one third of the cancer cases. The increase in goblet cells coincides with a loss of the more differentiated cells in the metaplastic glands, such as enterocytes, APUD cells, or Paneth cells. This \"enterocoli metaplasia\" seems to be specific for cancer bearing mucosa and occurs more often in cancer of intestinal type; it may represent a form of a derepressive dedifferentiation. The significance of enterocoli metaplasia as a premalignant lesion remains to be elucidated."}
{"id": "PMID:461233", "title": "Gastritis, intestinal metaplasia and dysplasia versus benign ulcer in stomach and duodenum and gastric carcinoma -- a histotopographical study.", "content": "In histological examination of gastrectomy specimens from patients with duodenal ulcer, gastric ulcer, and early and advanced cancer, both chronic atrophic gastritis and intestinal metaplasia were identified in 54% of the cases with duodenal ulcer. At 90 to 100%, respectively, these mucosal changes were approximately twice as frequent with gastric ulcer and early and advanced gastric cancer. Mild dysplasia occurred in 54% of the cases with duodenal ulcer; occurred somewhat more frequently with gastric ulcer, in 75% of the cases; and in almost all cases with early and advanced gastric cancer, at 90% and 100%, respectively. Whereas 27% of the cases with duodenal ulcer, 62% with gastric ulcer, and 90% and 95% of the respective cases with early and advanced gastric cancer showed moderate dysplasia, only severe dysplasia in early gastric cancer (40%) and advanced gastric (81%) was clearly more frequent in comparison to duodenal ulcer (9%) and gastric ulcer (12%). In the cases with duodenal ulcer chronic atrophic gastritis and intestinal metaplasia were limited mostly to the antrum; with gastric ulcer and cancerous stomach disorders, they also occurred in other stomach sections. Mild and moderate dysplasia conformed to the same distribution pattern. Severe dysplasia, which was only detected in two ulcer cases, was not only substantially more frequent in cases with early and advanced gastric cancer, but also showed a clear topographic relationship to cancer localization in the stomach.", "contents": "Gastritis, intestinal metaplasia and dysplasia versus benign ulcer in stomach and duodenum and gastric carcinoma -- a histotopographical study. In histological examination of gastrectomy specimens from patients with duodenal ulcer, gastric ulcer, and early and advanced cancer, both chronic atrophic gastritis and intestinal metaplasia were identified in 54% of the cases with duodenal ulcer. At 90 to 100%, respectively, these mucosal changes were approximately twice as frequent with gastric ulcer and early and advanced gastric cancer. Mild dysplasia occurred in 54% of the cases with duodenal ulcer; occurred somewhat more frequently with gastric ulcer, in 75% of the cases; and in almost all cases with early and advanced gastric cancer, at 90% and 100%, respectively. Whereas 27% of the cases with duodenal ulcer, 62% with gastric ulcer, and 90% and 95% of the respective cases with early and advanced gastric cancer showed moderate dysplasia, only severe dysplasia in early gastric cancer (40%) and advanced gastric (81%) was clearly more frequent in comparison to duodenal ulcer (9%) and gastric ulcer (12%). In the cases with duodenal ulcer chronic atrophic gastritis and intestinal metaplasia were limited mostly to the antrum; with gastric ulcer and cancerous stomach disorders, they also occurred in other stomach sections. Mild and moderate dysplasia conformed to the same distribution pattern. Severe dysplasia, which was only detected in two ulcer cases, was not only substantially more frequent in cases with early and advanced gastric cancer, but also showed a clear topographic relationship to cancer localization in the stomach."}
{"id": "PMID:461234", "title": "Incidence of epithelial dysplasia after partial gastric resection.", "content": "Because of the higher risk of cancer in the gastric stump, an increased incidence of pre-cancerous conditions should be exspected also in the resected stomach. Therefore, a combined endoscopic and bioptic study was performed in order to investigate the incidence of dysplasias in the gastric stump after resection for benign conditions. Among 101 patients with gastric resection, 2 cases were excluded from this study because of preceeding gastric cancer and one because of cancer of the gastric stump. In 43 of the remaining 98 patients, a Billroth-I-resection (gastroduodenostomy) had been carried out. In the remaining 55 patients with a Billroth-II-resection (gastroenterostomy) 9 had an additional enteroanastomosis of Braun whereas in the residual 46 patients this enteroanastomosis was lacking. This distinction was made because of a facultative or obligatory bile reflux. The average age of the B-I-group was 68 years, of the B-II-group with enteroanastomosis 69 years, and the B-II-group without enteroanastomosis 62 years. A non-operated group matched for age served as control group. Biopsy particles from the anastomotic region were gained by endoscopy and cut in step sections. The classification of dysplasias (degree I-III) followed the criteria given by Nagayo as modified by Grundmann. Inflammatory reactive changes were separated from these. A few changes could not be classified definitely and were listed as unclassified dysplasia. While dysplastic changes of low degree were quite numerous in every group, the dysplasias of higher degree were only found in a small number of cases. In the 46 cases with B-II-resection without Braun's enteroanastomosis, there were 5 dysplasia II and 3 dysplasia II. In the 9 cases with B-II-resection and with Braun's enteroanastomosis, there was 1 dysplasia I and no dysplasia III. In the 43 patient with B-I-resection only 2 dysplasia II and no dysplasia III were found. In the control group of 98 patients matched for age there were only 5 cases with dysplasia I and 1 case with dysplasia III. Patients with higher degrees of dysplasia showed a higher age and a longer interval after operation. There was also a correlation between higher degrees of dysplasia and severe atrophic changes in the mucosa. Correlating the degree of dysplasia with the reason for gastric resection, most of the dysplastic changes occurred in patients resected for gastric ulcer, whereas cases resected for duodenal ulcer showed only 2 dysplasias I. The discussion refers to the few data about dysplasia of the gastric stump available from the literature. Atrophic and increased regenerative changes obviously play a role in the pathogenesis of these dysplastic changes. As a causative factor the role of bile reflux is discussed. A further diagnostic and therapeutic regimen for the different forms of dysplasia is proposed.", "contents": "Incidence of epithelial dysplasia after partial gastric resection. Because of the higher risk of cancer in the gastric stump, an increased incidence of pre-cancerous conditions should be exspected also in the resected stomach. Therefore, a combined endoscopic and bioptic study was performed in order to investigate the incidence of dysplasias in the gastric stump after resection for benign conditions. Among 101 patients with gastric resection, 2 cases were excluded from this study because of preceeding gastric cancer and one because of cancer of the gastric stump. In 43 of the remaining 98 patients, a Billroth-I-resection (gastroduodenostomy) had been carried out. In the remaining 55 patients with a Billroth-II-resection (gastroenterostomy) 9 had an additional enteroanastomosis of Braun whereas in the residual 46 patients this enteroanastomosis was lacking. This distinction was made because of a facultative or obligatory bile reflux. The average age of the B-I-group was 68 years, of the B-II-group with enteroanastomosis 69 years, and the B-II-group without enteroanastomosis 62 years. A non-operated group matched for age served as control group. Biopsy particles from the anastomotic region were gained by endoscopy and cut in step sections. The classification of dysplasias (degree I-III) followed the criteria given by Nagayo as modified by Grundmann. Inflammatory reactive changes were separated from these. A few changes could not be classified definitely and were listed as unclassified dysplasia. While dysplastic changes of low degree were quite numerous in every group, the dysplasias of higher degree were only found in a small number of cases. In the 46 cases with B-II-resection without Braun's enteroanastomosis, there were 5 dysplasia II and 3 dysplasia II. In the 9 cases with B-II-resection and with Braun's enteroanastomosis, there was 1 dysplasia I and no dysplasia III. In the 43 patient with B-I-resection only 2 dysplasia II and no dysplasia III were found. In the control group of 98 patients matched for age there were only 5 cases with dysplasia I and 1 case with dysplasia III. Patients with higher degrees of dysplasia showed a higher age and a longer interval after operation. There was also a correlation between higher degrees of dysplasia and severe atrophic changes in the mucosa. Correlating the degree of dysplasia with the reason for gastric resection, most of the dysplastic changes occurred in patients resected for gastric ulcer, whereas cases resected for duodenal ulcer showed only 2 dysplasias I. The discussion refers to the few data about dysplasia of the gastric stump available from the literature. Atrophic and increased regenerative changes obviously play a role in the pathogenesis of these dysplastic changes. As a causative factor the role of bile reflux is discussed. A further diagnostic and therapeutic regimen for the different forms of dysplasia is proposed."}
{"id": "PMID:461235", "title": "Early gastric cancer: a morphological study of 144 cases.", "content": "Within 144 early gastric cancers (EGC), the ulcerated type was most frequent (54.2%), followed by the superfical (29.9%) and protruded types (16%). 10.4% of EGC originated from adenomatous polyps of gastric mucosa. No positive correlation between hyperplastic polyps and occurrence of EGC could be stated. Antrum and corpus regions were preferred localization of EGC, harboring about 90% of the tumors. Histologic classification according to Laur\u00e9n (1965) proved to be very reliable, revealing an intestinal type of EGC in 53.1% and a diffuse type in 32.4%. Primary anaplastic tumors seem to be very rare. Intestinal types were predominant in males, especially in the elderly patient. In about 50% the tumor was still limited to the mucosa with the exception of Type I, since those tumors originating from polyp infiltrated submucosa above and those without evidence of a preceding polyp, below average.  Regional metastases were found in 4.2% with one primary tumor still limited to the mucosa. In the pure intestinal type of EGC metastases did not occur at all. Multicentricity of EGC was, with 2.8%, remarkably low, compared with the findings of others. Excluding malignancies, the only other noteworthy concurrent lesions were ulcer scars or chronic ulcers with 6.9% and B II resection for benign disease with 2.8%.", "contents": "Early gastric cancer: a morphological study of 144 cases. Within 144 early gastric cancers (EGC), the ulcerated type was most frequent (54.2%), followed by the superfical (29.9%) and protruded types (16%). 10.4% of EGC originated from adenomatous polyps of gastric mucosa. No positive correlation between hyperplastic polyps and occurrence of EGC could be stated. Antrum and corpus regions were preferred localization of EGC, harboring about 90% of the tumors. Histologic classification according to Laur\u00e9n (1965) proved to be very reliable, revealing an intestinal type of EGC in 53.1% and a diffuse type in 32.4%. Primary anaplastic tumors seem to be very rare. Intestinal types were predominant in males, especially in the elderly patient. In about 50% the tumor was still limited to the mucosa with the exception of Type I, since those tumors originating from polyp infiltrated submucosa above and those without evidence of a preceding polyp, below average.  Regional metastases were found in 4.2% with one primary tumor still limited to the mucosa. In the pure intestinal type of EGC metastases did not occur at all. Multicentricity of EGC was, with 2.8%, remarkably low, compared with the findings of others. Excluding malignancies, the only other noteworthy concurrent lesions were ulcer scars or chronic ulcers with 6.9% and B II resection for benign disease with 2.8%."}
{"id": "PMID:461236", "title": "Is there a carcinoma in situ of gastric mucosa?", "content": "Carcinoma in situ, a precancerous lesion in the strict sense, was first recognized in stratified squamous epithelia. It is characterized by markedly atypical cells replacing the autochthonous epithelial cells without stromal invasion, the basement membrane being well preserved. Notwithstanding gradual transitions between carcinoma in situ on the one hand and dysplasias and invasive cancer on the other hand, its histological separation from the latter is feasible in the uterine portio. Its recognition has decisive therapeutic and prognostic implications, particularly in view of the frequently observed latent period between purely superficial spread and early invasive growth. In contrast difficulties are encountered in applying the concept of Carcinoma in situ to mucosal lesions of the stomach, though an analogous replacement stage has to be postulated, because the gastric and cervical mucous membranes differ fundamentally in their structural characteristics. The epithelial cells of the surface, pits and tubular glands do exhibit progressive atypia during cancerization. However, the single-layered epithelium offers far less distinctive criteria than the stratified squamous epithelium. Newly formed glandular complexes cannot be accepted as evidence for in situ growth. The latent period between purely superficial replacement by atypical cells and invasion appears to be considerably shorter in the stomach than in the portio, probably because the mechanical resistance of the loosely textured gastric lamina propria is small. In addition, stromal invasion may originate from any one epithelial cell in the gastric mucosa, whilst it is just the basal layer from which invasively growing cells may emanate in the uterine portio. The occurrence of dysplasia in the mucosa of the stomach does not justify a gastrectomy, according to our current experience, there being no intervention in gastric surgery equivalent to that of conization.", "contents": "Is there a carcinoma in situ of gastric mucosa? Carcinoma in situ, a precancerous lesion in the strict sense, was first recognized in stratified squamous epithelia. It is characterized by markedly atypical cells replacing the autochthonous epithelial cells without stromal invasion, the basement membrane being well preserved. Notwithstanding gradual transitions between carcinoma in situ on the one hand and dysplasias and invasive cancer on the other hand, its histological separation from the latter is feasible in the uterine portio. Its recognition has decisive therapeutic and prognostic implications, particularly in view of the frequently observed latent period between purely superficial spread and early invasive growth. In contrast difficulties are encountered in applying the concept of Carcinoma in situ to mucosal lesions of the stomach, though an analogous replacement stage has to be postulated, because the gastric and cervical mucous membranes differ fundamentally in their structural characteristics. The epithelial cells of the surface, pits and tubular glands do exhibit progressive atypia during cancerization. However, the single-layered epithelium offers far less distinctive criteria than the stratified squamous epithelium. Newly formed glandular complexes cannot be accepted as evidence for in situ growth. The latent period between purely superficial replacement by atypical cells and invasion appears to be considerably shorter in the stomach than in the portio, probably because the mechanical resistance of the loosely textured gastric lamina propria is small. In addition, stromal invasion may originate from any one epithelial cell in the gastric mucosa, whilst it is just the basal layer from which invasively growing cells may emanate in the uterine portio. The occurrence of dysplasia in the mucosa of the stomach does not justify a gastrectomy, according to our current experience, there being no intervention in gastric surgery equivalent to that of conization."}
{"id": "PMID:461275", "title": "Approaches towards rational antiviral chemotherapy.", "content": "Present epidemic influenza is uncontrolled by immuno- or chemoprophylaxis. Mutants of varying antigenic composition arise with relatively high frequency in nature and are able to circumvent herd, or induced, immunity. Also, drug-resistant viruses can be selected in vitro and this resistance can be exchanged to other viruses by gene reassortment. Combined immuno- and chemoprophylaxis may provide a more effective approach to the ultimate control of the disease. Most antiviral compounds have been selected by random screening in the laboratory. Application of more specific enzyme assays such as the virion-associated RNA transcriptase assays may produce other compounds with a defined mode of action - semi-rational chemotherapy. RNA and polypeptide sequence studies are in progress elsewhere to define transcription and translation initiation sites or virus adsorption sites. Such knowledge could lead to a new generation of antiviral compounds. Specific delivery of virus inhibitory compounds is an interesting problem. Liposomes are lipid spheres, and these have been used for the delivery of antiviral compounds.", "contents": "Approaches towards rational antiviral chemotherapy. Present epidemic influenza is uncontrolled by immuno- or chemoprophylaxis. Mutants of varying antigenic composition arise with relatively high frequency in nature and are able to circumvent herd, or induced, immunity. Also, drug-resistant viruses can be selected in vitro and this resistance can be exchanged to other viruses by gene reassortment. Combined immuno- and chemoprophylaxis may provide a more effective approach to the ultimate control of the disease. Most antiviral compounds have been selected by random screening in the laboratory. Application of more specific enzyme assays such as the virion-associated RNA transcriptase assays may produce other compounds with a defined mode of action - semi-rational chemotherapy. RNA and polypeptide sequence studies are in progress elsewhere to define transcription and translation initiation sites or virus adsorption sites. Such knowledge could lead to a new generation of antiviral compounds. Specific delivery of virus inhibitory compounds is an interesting problem. Liposomes are lipid spheres, and these have been used for the delivery of antiviral compounds."}
{"id": "PMID:461276", "title": "The human enteric coronaviruses.", "content": "A coronarirus was seen in the faeces from 15 (4.2%) of 355 adults with diarrhoea and from 5 (5.2%) of 96 adults without diarrhoea. Similar particles were seen in the faeces from 5 (2.2%) of 227 children aged 1--14 years with gastroenteritis, but in none of those from 230 infants under one year of age with gastroenteritis. There was no evidence that the coronavirus was responsible for any of 34 outbreaks of gastroenteritis, although it possibly caused diarrhoea in patients admitted to a psycho-geriatric unit. Excretion of the virus often continued for many months. One strain was propagated in human embryo kidney monolayers and human embryo intestinal organ cultures, although serial passage could not be accomplished.", "contents": "The human enteric coronaviruses. A coronarirus was seen in the faeces from 15 (4.2%) of 355 adults with diarrhoea and from 5 (5.2%) of 96 adults without diarrhoea. Similar particles were seen in the faeces from 5 (2.2%) of 227 children aged 1--14 years with gastroenteritis, but in none of those from 230 infants under one year of age with gastroenteritis. There was no evidence that the coronavirus was responsible for any of 34 outbreaks of gastroenteritis, although it possibly caused diarrhoea in patients admitted to a psycho-geriatric unit. Excretion of the virus often continued for many months. One strain was propagated in human embryo kidney monolayers and human embryo intestinal organ cultures, although serial passage could not be accomplished."}
{"id": "PMID:461277", "title": "The search for the ideal influenza vaccine.", "content": "The history of the development of influenza virus vaccine is traced from its origin with experimental studies of influenza virus in ferrets and mice and the first trials in man. Knowledge of the basis of immunity to the viruses in experimental animals and in man has grown steadily over the years and has been essential to successful immunization. Virus variation affecting the surface antigens of the virus is seen as the principal obstacle to the application of vaccines in man. So significant are the changes occurring during antigenic drift that former concepts of a polyvalent vaccine cannot provide a solution of the problem of the composition of vaccines. Disrupted virus vaccines appear to provide the answer to the prevention of vaccine reactions.", "contents": "The search for the ideal influenza vaccine. The history of the development of influenza virus vaccine is traced from its origin with experimental studies of influenza virus in ferrets and mice and the first trials in man. Knowledge of the basis of immunity to the viruses in experimental animals and in man has grown steadily over the years and has been essential to successful immunization. Virus variation affecting the surface antigens of the virus is seen as the principal obstacle to the application of vaccines in man. So significant are the changes occurring during antigenic drift that former concepts of a polyvalent vaccine cannot provide a solution of the problem of the composition of vaccines. Disrupted virus vaccines appear to provide the answer to the prevention of vaccine reactions."}
{"id": "PMID:461278", "title": "Antigenic memory to influenza A viruses in man determined by monovalent vaccines.", "content": "This study was conducted to test the limits of the doctrine of 'original antigenic sin' in influenza A. The design included use of zonal purified 1000 CCA (chick cell agglutinating) units monovalent vaccines consisting of H0N1, H1N1, H2N2 and H3N2. Age cohorts with different primary influenza A infections were established for the 687 volunteers. The vaccines administered to each age cohort were selected to test the responsiveness of original antigenic sin antibody to homologous and heterologous challenge. Anamnestic responses were demonstrated with Hsw1n1,H0N1, and H1N1 and with H2N2 and H3N2 but not between the groups. The synthesis of these findings is that there are 2 original antigenic sins - 2 families of influenza A viruses.", "contents": "Antigenic memory to influenza A viruses in man determined by monovalent vaccines. This study was conducted to test the limits of the doctrine of 'original antigenic sin' in influenza A. The design included use of zonal purified 1000 CCA (chick cell agglutinating) units monovalent vaccines consisting of H0N1, H1N1, H2N2 and H3N2. Age cohorts with different primary influenza A infections were established for the 687 volunteers. The vaccines administered to each age cohort were selected to test the responsiveness of original antigenic sin antibody to homologous and heterologous challenge. Anamnestic responses were demonstrated with Hsw1n1,H0N1, and H1N1 and with H2N2 and H3N2 but not between the groups. The synthesis of these findings is that there are 2 original antigenic sins - 2 families of influenza A viruses."}
{"id": "PMID:461279", "title": "Pancreatitis in childhood.", "content": "Follow-up of 25 cases of pancreatitis in childhood ascertained from the Hospital Activity Analysis in Newcastle and Wales showed that the majority of the children thrived after their illness. Only one child died. Only 2 children developed diabetes mellitus and 3 had significant malabsorption. There were 13 idiopathic cases (9 acute, 4 chronic relapsing), 3 of which were obese girls of pubertal age. It is speculated that obesity, puberty and female sex together may predispose to acute pancreatitis.", "contents": "Pancreatitis in childhood. Follow-up of 25 cases of pancreatitis in childhood ascertained from the Hospital Activity Analysis in Newcastle and Wales showed that the majority of the children thrived after their illness. Only one child died. Only 2 children developed diabetes mellitus and 3 had significant malabsorption. There were 13 idiopathic cases (9 acute, 4 chronic relapsing), 3 of which were obese girls of pubertal age. It is speculated that obesity, puberty and female sex together may predispose to acute pancreatitis."}
{"id": "PMID:461280", "title": "Bile acid, neutral sterol and faecal fat excretion in subjects treated with fenfluramine and its relationship to fenfluramine-induced diarrhoea.", "content": "Bile acid, neutral sterol and faecal fat excretion was studied over a period of 9 weeks in a group of 16 healthy subjects before, during and after administration of fenfluramine. Statistical analysis revealed a significant increase in bile acid excretion during the drug phase (P less than 0.02); and during recovery period of 3 weeks (P less than 0.05). Faecal neutral sterol, as the total of coprostanol and cholesterol elimination was also enhanced after fenfluramine. Coprostanol was replaced by cholesterol in 12 subjects. Faecal fat was studied in 6 subjects, the excretion increased during the drug phase (P less than 0.05), and remained elevated during the post-drug period (P less than 0.01). The composition of the bile acids remained unaltered in all the subjects except 3 who had a fenfluramine-induced watery diarrhoea; and these excreted chenodeoxy and cholic together with smaller amounts of secondary bile acids. A higher excretion of bile acids was found in the 8 overweight subjects (P less than 0.01) before ministration of fenfluramine. These results are discussed in an attempt to correlate the effect of fenfluramine with changes in bile acid and neutral sterol excretion, and its relationship to fenfluramine-induced diarrhoea.", "contents": "Bile acid, neutral sterol and faecal fat excretion in subjects treated with fenfluramine and its relationship to fenfluramine-induced diarrhoea. Bile acid, neutral sterol and faecal fat excretion was studied over a period of 9 weeks in a group of 16 healthy subjects before, during and after administration of fenfluramine. Statistical analysis revealed a significant increase in bile acid excretion during the drug phase (P less than 0.02); and during recovery period of 3 weeks (P less than 0.05). Faecal neutral sterol, as the total of coprostanol and cholesterol elimination was also enhanced after fenfluramine. Coprostanol was replaced by cholesterol in 12 subjects. Faecal fat was studied in 6 subjects, the excretion increased during the drug phase (P less than 0.05), and remained elevated during the post-drug period (P less than 0.01). The composition of the bile acids remained unaltered in all the subjects except 3 who had a fenfluramine-induced watery diarrhoea; and these excreted chenodeoxy and cholic together with smaller amounts of secondary bile acids. A higher excretion of bile acids was found in the 8 overweight subjects (P less than 0.01) before ministration of fenfluramine. These results are discussed in an attempt to correlate the effect of fenfluramine with changes in bile acid and neutral sterol excretion, and its relationship to fenfluramine-induced diarrhoea."}
{"id": "PMID:461281", "title": "Influenza in a geriatric unit.", "content": "An outbreak of influenza A (resembling A/Victoria/3/75) occurred in 2 geriatric wards in February 1976 and, out of 19 patients, 3 died. Of the patients who were at risk, 30% were affected. Immunization for influenza in the elderly just before the winter is suggested. The procedure is simple and inexpensive. The recommendations of the DHSS (U.K.) for the use of influenza vaccine to practising doctors is, therefore, welcome. The suitable treatment appears to be that of repeated immunization with inactivated vaccine. Amantadine in the elderly population may also be effective, and is worthy of trial.", "contents": "Influenza in a geriatric unit. An outbreak of influenza A (resembling A/Victoria/3/75) occurred in 2 geriatric wards in February 1976 and, out of 19 patients, 3 died. Of the patients who were at risk, 30% were affected. Immunization for influenza in the elderly just before the winter is suggested. The procedure is simple and inexpensive. The recommendations of the DHSS (U.K.) for the use of influenza vaccine to practising doctors is, therefore, welcome. The suitable treatment appears to be that of repeated immunization with inactivated vaccine. Amantadine in the elderly population may also be effective, and is worthy of trial."}
{"id": "PMID:461282", "title": "Progressive systemic sclerosis in the elderly.", "content": "There is evidence to suggest that the incidence of progressive systemic sclerosis in the elderly is more common than in younger age groups. As in younger patients, late onset cases may have minimal skin changes and this can cause difficulty in diagnosis. The case histories of 2 patients are presented to illustrate these points.", "contents": "Progressive systemic sclerosis in the elderly. There is evidence to suggest that the incidence of progressive systemic sclerosis in the elderly is more common than in younger age groups. As in younger patients, late onset cases may have minimal skin changes and this can cause difficulty in diagnosis. The case histories of 2 patients are presented to illustrate these points."}
{"id": "PMID:461283", "title": "Riedel's thyroiditis leading to severe but reversible pituitary failure.", "content": "This patient with histologically proved Riedel's thyroiditis had hypothyroidism of sufficient severity to cause panhypopituitarism, and it was this which first brought him to medical attention. The beneficial effects are described of surgery in relieving severe dysphagia and stridor, and of prednisone in softening and shrinking the hard neck mass. The extent of pituitary failure, and the degree of recovery on treatment with thyroxine are reported, together with details of 4 other cases of Riedel's thyroiditis seen by one of the authors (S.T.).", "contents": "Riedel's thyroiditis leading to severe but reversible pituitary failure. This patient with histologically proved Riedel's thyroiditis had hypothyroidism of sufficient severity to cause panhypopituitarism, and it was this which first brought him to medical attention. The beneficial effects are described of surgery in relieving severe dysphagia and stridor, and of prednisone in softening and shrinking the hard neck mass. The extent of pituitary failure, and the degree of recovery on treatment with thyroxine are reported, together with details of 4 other cases of Riedel's thyroiditis seen by one of the authors (S.T.)."}
{"id": "PMID:461284", "title": "Ventricular fibrillation following high potency intravenous vitamin injection.", "content": "A 67-year-old woman developed ventricular fibrillation following Parenterovite injection. Subsequent post-mortem revealed normal coronary arteries. It is postulated that the high potency vitamin injection caused the arrhythmia.", "contents": "Ventricular fibrillation following high potency intravenous vitamin injection. A 67-year-old woman developed ventricular fibrillation following Parenterovite injection. Subsequent post-mortem revealed normal coronary arteries. It is postulated that the high potency vitamin injection caused the arrhythmia."}
{"id": "PMID:461285", "title": "Sarcoma obstructing right ventricular cavity: clinical, echocardiographic, haemodynamic and angiographic features.", "content": "Right atrial hypertension and pericardial effusion developed 2 years after mastectomy for fibrosarcoma. Clinical and echocardiographic features suggested right ventricular tumour. At catheterization the right ventricular cavity was almost obliterated, with an infundibular gradient of 13 mmHg, and biopsy of the mass was attempted. Post-mortem revealed extensive infiltrating and intracavitary right ventricular fibrosarcoma without extracardiac tumour.", "contents": "Sarcoma obstructing right ventricular cavity: clinical, echocardiographic, haemodynamic and angiographic features. Right atrial hypertension and pericardial effusion developed 2 years after mastectomy for fibrosarcoma. Clinical and echocardiographic features suggested right ventricular tumour. At catheterization the right ventricular cavity was almost obliterated, with an infundibular gradient of 13 mmHg, and biopsy of the mass was attempted. Post-mortem revealed extensive infiltrating and intracavitary right ventricular fibrosarcoma without extracardiac tumour."}
{"id": "PMID:461286", "title": "Interatrial abscess.", "content": "A large abscess of the interatrial septum developed during the course of acute bacterial endocarditis affecting the aortic valve. Septic involvement of the cardiac conducting system produced atrioventricular dissociation, and subsequent abscess rupture resulted in an aorto-right atrial communication. The clinical presentation of this rare complication of acute bacterial endocarditis is correlated with the post-mortem findings.", "contents": "Interatrial abscess. A large abscess of the interatrial septum developed during the course of acute bacterial endocarditis affecting the aortic valve. Septic involvement of the cardiac conducting system produced atrioventricular dissociation, and subsequent abscess rupture resulted in an aorto-right atrial communication. The clinical presentation of this rare complication of acute bacterial endocarditis is correlated with the post-mortem findings."}
{"id": "PMID:461287", "title": "Isolated perforation of the gall bladder following blunt abdominal trauma.", "content": "Isolated perforation of the gall bladder as a consequence of blunt abdominal injury is rare. A single case is described which illustrates several features which may characterize this lesion.", "contents": "Isolated perforation of the gall bladder following blunt abdominal trauma. Isolated perforation of the gall bladder as a consequence of blunt abdominal injury is rare. A single case is described which illustrates several features which may characterize this lesion."}
{"id": "PMID:461288", "title": "Chronic ulcerative jejunitis without symptoms.", "content": "A patient is presented who, in addition to subtotal villous atrophy, had superficial ulcers of the jejunum, as well as a wide band of granulation tissue deep to the crypts suggesting recurrent past ulceration. In spite of these changes she had no intestinal symptoms at the time of her presentation with reflux oesophagitis, and her only nutritional abnormality was a mild folate deficiency. Withdrawal of dietary gluten produced some improvement of the jejunal mucosa but this was not sustained on continued low-grade ingestion. She has containued to be virtually symptom-free over a 3-year period of follow-up.", "contents": "Chronic ulcerative jejunitis without symptoms. A patient is presented who, in addition to subtotal villous atrophy, had superficial ulcers of the jejunum, as well as a wide band of granulation tissue deep to the crypts suggesting recurrent past ulceration. In spite of these changes she had no intestinal symptoms at the time of her presentation with reflux oesophagitis, and her only nutritional abnormality was a mild folate deficiency. Withdrawal of dietary gluten produced some improvement of the jejunal mucosa but this was not sustained on continued low-grade ingestion. She has containued to be virtually symptom-free over a 3-year period of follow-up."}
{"id": "PMID:461289", "title": "Leiomyoma of the duodenum--an unusual presentation of a rare tumour.", "content": "A case of leiomyoma of the duodenum presenting with sub-acute intestinal obstruction and a palpable abdominal mass is described. Both the siting of the lesion in the duodenum and a presentation other than gastro-intestinal bleeding are unusual for this type of tumour.", "contents": "Leiomyoma of the duodenum--an unusual presentation of a rare tumour. A case of leiomyoma of the duodenum presenting with sub-acute intestinal obstruction and a palpable abdominal mass is described. Both the siting of the lesion in the duodenum and a presentation other than gastro-intestinal bleeding are unusual for this type of tumour."}
{"id": "PMID:461290", "title": "[Psychosomatic medicine in intensive care units (author's transl)].", "content": "Psychosomatic medicine in intensive care units is essentially characterized by problems arising from the emotional involvement of the intensive care unit team. That is why the functions of a psychosomaticist are not only the diagnostics of psychosyndromes and the psychotherapy of dangerously ill patients, but especially the study of psychological interrelations within the unit team. Hypochondriac depressive psychosyndromes caused by the experience of dread, confusion, exhaustion and communication problems, as well as psychosyndromes characterized by a reduction of consciousness and orientation are discussed. Anaclitic psychotherapy, i.e. supporting and encouraging care, and emergency psychotherapy actually required in precarious situations, are described. The specific interrelations within and between the individual groups of the intensive care unit team (physicians, female and male nurses), which are important to the working conditions in the unit, and the possibilities of their psychological activation (e. g. in Balint groups) are outlined. The status and responsibility of a psychosomaticist within the unit team is critically reflected. Finally, the permanent confrontation of the team with death and dying is emphasized, and the psychological aspect of discontinuing intensive care is discussed.", "contents": "[Psychosomatic medicine in intensive care units (author's transl)]. Psychosomatic medicine in intensive care units is essentially characterized by problems arising from the emotional involvement of the intensive care unit team. That is why the functions of a psychosomaticist are not only the diagnostics of psychosyndromes and the psychotherapy of dangerously ill patients, but especially the study of psychological interrelations within the unit team. Hypochondriac depressive psychosyndromes caused by the experience of dread, confusion, exhaustion and communication problems, as well as psychosyndromes characterized by a reduction of consciousness and orientation are discussed. Anaclitic psychotherapy, i.e. supporting and encouraging care, and emergency psychotherapy actually required in precarious situations, are described. The specific interrelations within and between the individual groups of the intensive care unit team (physicians, female and male nurses), which are important to the working conditions in the unit, and the possibilities of their psychological activation (e. g. in Balint groups) are outlined. The status and responsibility of a psychosomaticist within the unit team is critically reflected. Finally, the permanent confrontation of the team with death and dying is emphasized, and the psychological aspect of discontinuing intensive care is discussed."}
{"id": "PMID:461291", "title": "[A retrospective study of the impressions and psychic reactions experienced by patients in a traumatological intensive care unit (author's transl)].", "content": "30 persons who had been treated for an average of 22 days on a traumatological intensive care unit were asked to comment on their impressions and experiences. 80% of the patients had been in need of artificial respiration; but this had not unduly distressed them and they remembered it only vaguely. The major worry in 30% of the cases was awareness of their condition and the fear of permanent physical and mental disablement. 25% had greatly suffered from thirst. There was no mention that finding themselves in an intensive care ward, being dependent on a respirator or monitoring equipment or being disorientated had caused distress. None of them had seriously contemplated the possibility of his own death. The frequent visits by relatives were regarded as great comfort and help towards achieving mental equilibrium. All these critically ill persons were highly impressed by the constant and dedicated medical and nursing care they had received.", "contents": "[A retrospective study of the impressions and psychic reactions experienced by patients in a traumatological intensive care unit (author's transl)]. 30 persons who had been treated for an average of 22 days on a traumatological intensive care unit were asked to comment on their impressions and experiences. 80% of the patients had been in need of artificial respiration; but this had not unduly distressed them and they remembered it only vaguely. The major worry in 30% of the cases was awareness of their condition and the fear of permanent physical and mental disablement. 25% had greatly suffered from thirst. There was no mention that finding themselves in an intensive care ward, being dependent on a respirator or monitoring equipment or being disorientated had caused distress. None of them had seriously contemplated the possibility of his own death. The frequent visits by relatives were regarded as great comfort and help towards achieving mental equilibrium. All these critically ill persons were highly impressed by the constant and dedicated medical and nursing care they had received."}
{"id": "PMID:461292", "title": "[\"Locked-in\" syndrome in intensive care patients (author's transl)].", "content": "The \"locked-in\" syndrome constitutes a potential source of diagnostic and prognostic errors, especially in intensive care patients. The pathologicoanatomical, pathophysiological and clinical features of the syndrome are described and differentiated from other syndromes. Means of establishing contact are reviewed which would allow simple wishes on the part of the patient to be satisfied and thus prevent the psychic isolation of the (conscious) patient.", "contents": "[\"Locked-in\" syndrome in intensive care patients (author's transl)]. The \"locked-in\" syndrome constitutes a potential source of diagnostic and prognostic errors, especially in intensive care patients. The pathologicoanatomical, pathophysiological and clinical features of the syndrome are described and differentiated from other syndromes. Means of establishing contact are reviewed which would allow simple wishes on the part of the patient to be satisfied and thus prevent the psychic isolation of the (conscious) patient."}
{"id": "PMID:461293", "title": "[A case of severe multiple trauma (author's transl)].", "content": "A case of severe multiple trauma sustained in a road accident is reported. The aim of the report is to show the determing role played by diagnostic and surgical methods and therapeutic possibilities available in the intensive care unit (shock treatment, controlled respiration, parenteral feeding, haemodialysis) in winning the five-months' battle for the life of the patient. Early haemodyalisis as supporting therapy in respiratory failure and conservation treatment of intestinal fistulae are important. Post-traumatic pancreatitis which is a not infrequent complicating feature of severe multiple trauma may present diagnostic difficulties.", "contents": "[A case of severe multiple trauma (author's transl)]. A case of severe multiple trauma sustained in a road accident is reported. The aim of the report is to show the determing role played by diagnostic and surgical methods and therapeutic possibilities available in the intensive care unit (shock treatment, controlled respiration, parenteral feeding, haemodialysis) in winning the five-months' battle for the life of the patient. Early haemodyalisis as supporting therapy in respiratory failure and conservation treatment of intestinal fistulae are important. Post-traumatic pancreatitis which is a not infrequent complicating feature of severe multiple trauma may present diagnostic difficulties."}
{"id": "PMID:461294", "title": "[EEG-spectrum analysis for documentation of depth of anaesthesia. Correlation with pharmacokinetic data of fentanyl (author's transl)].", "content": "The article reports on a study regarding the posibility of monitoring anaesthesia via EEG characteristics when using fentanyl-nitrous oxide. An alpha-delta index correlated sufficiently accurately with the plasma levels of fentanyl administered via continuous infusion.", "contents": "[EEG-spectrum analysis for documentation of depth of anaesthesia. Correlation with pharmacokinetic data of fentanyl (author's transl)]. The article reports on a study regarding the posibility of monitoring anaesthesia via EEG characteristics when using fentanyl-nitrous oxide. An alpha-delta index correlated sufficiently accurately with the plasma levels of fentanyl administered via continuous infusion."}
{"id": "PMID:461295", "title": "[Physical basis and therapeutic range of application of ultrasonic aerosols (author's transl)].", "content": "An aerosol of an aqueous solution still changes its size from development up to alveoli; therefore only conclusions can be made about possible sizes of particle spectrum. Volatilisation, condensation, coagulation and sedimentation are the important interfering factors. To characterize an aerosol one should know the aerosolvolume, the quantity of nebulised substance and the relative size distribution. A method for measurement of size distribution -- a radioactive aerosol was deposited in a spiral centrifuge -- is described and the aerosol of two ultrasonic nebulizers were compared. Because of their physical properties aerosols of ultrasonic nebulizers play a main part in prophylaxis and therapy of functional lesion of bronchopulmonary affections.", "contents": "[Physical basis and therapeutic range of application of ultrasonic aerosols (author's transl)]. An aerosol of an aqueous solution still changes its size from development up to alveoli; therefore only conclusions can be made about possible sizes of particle spectrum. Volatilisation, condensation, coagulation and sedimentation are the important interfering factors. To characterize an aerosol one should know the aerosolvolume, the quantity of nebulised substance and the relative size distribution. A method for measurement of size distribution -- a radioactive aerosol was deposited in a spiral centrifuge -- is described and the aerosol of two ultrasonic nebulizers were compared. Because of their physical properties aerosols of ultrasonic nebulizers play a main part in prophylaxis and therapy of functional lesion of bronchopulmonary affections."}
{"id": "PMID:461296", "title": "[The treatment of haemorrhagic complications due to disturbance of platelet function after operations with extracorporal circulation (author's transl)].", "content": "The preoperative selective blood cell separation followed by the postoperative platelet autoransfusion could prevent the exhaustion of platelet function by operations with extracorporal circulation. The postoperative blood loss could be reduced by about 57%. This method can be recommended specially in cases of increased platelet traumatization after long time perfusion. Partial disturbances of platelet function after operations with ECC can also be favourably influenced by infusion of homologous phospholipid (Fibraccel). The postoperative blood loss could be reduced by about one third. This method is beneficial in open heart surgery with short perfusion times due to its little technical expenses.", "contents": "[The treatment of haemorrhagic complications due to disturbance of platelet function after operations with extracorporal circulation (author's transl)]. The preoperative selective blood cell separation followed by the postoperative platelet autoransfusion could prevent the exhaustion of platelet function by operations with extracorporal circulation. The postoperative blood loss could be reduced by about 57%. This method can be recommended specially in cases of increased platelet traumatization after long time perfusion. Partial disturbances of platelet function after operations with ECC can also be favourably influenced by infusion of homologous phospholipid (Fibraccel). The postoperative blood loss could be reduced by about one third. This method is beneficial in open heart surgery with short perfusion times due to its little technical expenses."}
{"id": "PMID:461297", "title": "[Skin emphysema caused by a sump tube (author's transl)].", "content": "A case of a transnasal, paraoesophageally placed sump tube is reported with consecutive skin emphysema. This complication was caused by certain circumstances: 1. unnoticed perforation of oesophagus, 2. open tube, 3. inspiration against resistance, 4. tube tip placed in slack connective tissue.", "contents": "[Skin emphysema caused by a sump tube (author's transl)]. A case of a transnasal, paraoesophageally placed sump tube is reported with consecutive skin emphysema. This complication was caused by certain circumstances: 1. unnoticed perforation of oesophagus, 2. open tube, 3. inspiration against resistance, 4. tube tip placed in slack connective tissue."}
{"id": "PMID:461306", "title": "[Clinical diagnosis of allergy (author's transl)].", "content": "\"Allergic\" manifestations are not necessarily of allergic origin; they can also be produced by non-allergic factors and their true allergic nature must, therefore, be proved. A detailed personal history and the presence of eosinophilia (in the blood or secretions) are suggestive evidence which has to be confirmed by the identification of specific antibodies of the immunoglobulin-E type. Criteria for arriving at the diagnosis \"allergy\" are (a) demonstration of antibodies by skin and mucous membrane tests with allergen extracts; (b) demonstration of the activity of the identified allergen by challenge tests on the reacting organ (conjunctiva, nose, bronchi); (c) in-vitro demonstration of the allergen-specific immunoglobulin-E by means of the radio-allergo-sorbent test. Details for the performance of the various tests are given and the interpretation and significance of the findings are discussed.", "contents": "[Clinical diagnosis of allergy (author's transl)]. \"Allergic\" manifestations are not necessarily of allergic origin; they can also be produced by non-allergic factors and their true allergic nature must, therefore, be proved. A detailed personal history and the presence of eosinophilia (in the blood or secretions) are suggestive evidence which has to be confirmed by the identification of specific antibodies of the immunoglobulin-E type. Criteria for arriving at the diagnosis \"allergy\" are (a) demonstration of antibodies by skin and mucous membrane tests with allergen extracts; (b) demonstration of the activity of the identified allergen by challenge tests on the reacting organ (conjunctiva, nose, bronchi); (c) in-vitro demonstration of the allergen-specific immunoglobulin-E by means of the radio-allergo-sorbent test. Details for the performance of the various tests are given and the interpretation and significance of the findings are discussed."}
{"id": "PMID:461308", "title": "[Extrinsic allergy in obstructive respiratory disease in childhood and adolescence (author's transl)].", "content": "The incidence of extrinsic allergy was studied in 1,246 children and adolescents with recurrent unspecific respiratory and allergic complaints. Special attention was paid to obstructive respiratory diseases, such as bronchial asthma and obstructive of asthmatic bronchitis. Skin tests and the radio-allergo-sorbent test (RAST) showed sensitization in 77% of the cases. Challenge tests and a detailed personal history, however, revealed clinically active sensitization in only 62%. The clinical and practical significance of the findings is discussed.", "contents": "[Extrinsic allergy in obstructive respiratory disease in childhood and adolescence (author's transl)]. The incidence of extrinsic allergy was studied in 1,246 children and adolescents with recurrent unspecific respiratory and allergic complaints. Special attention was paid to obstructive respiratory diseases, such as bronchial asthma and obstructive of asthmatic bronchitis. Skin tests and the radio-allergo-sorbent test (RAST) showed sensitization in 77% of the cases. Challenge tests and a detailed personal history, however, revealed clinically active sensitization in only 62%. The clinical and practical significance of the findings is discussed."}
{"id": "PMID:461309", "title": "[The clinical significance of challenge tests (author's transl)].", "content": "108 pollen-sensitive persons (positive skin test, positive history) and 40 latent pollen-allergic persons (positive skin test, but no conjunctival or nasal manifestations during the flowering season) were given intranasally increasing concentrations of pollen extract until a reaction occurred (itching, sneezing, running and blocked nose). 7.4% of the patients reacted to 0.001% w/v of the pollen extract, 56.5% reacted to 0.01, and 36% to a dose of 0.1% w/v. The corresponding figures for persons with latent allergy were 0.7, 5 and 30%; 62.5% reacted to 1.0 or 2.5% w/v of the extract. There was a relatively sharp division between doses of 0.1 and 1.0% w/v: persons who responded only to 1% concentrations invariably belonged to the group of latent allergy. 35 persons with positive skin tests to dust mites were divided into 3 groups: those with either a positive or a negative history of dust allergy and those concentrations of dust mite extracts (0.012, 0.12 and 1.2% w/v). Patients were considered to have latent allergy if they were sensitive to dust mite but were free from symptoms; or if they were sensitive to dust mite and pollen, but were symptom-free outside the flowering season. Although the group reacted slightly more often only to concentrations of 1.2%, the nasal response occurred on the whole with the same frequency with all three concentrations. In contrast to pollen allergy a positive intranasal dust mite test is not necessarily clinically relevant. Provocation tests are, therefore, not an infallible means to ascertain the presence of clinically relevant allergy.", "contents": "[The clinical significance of challenge tests (author's transl)]. 108 pollen-sensitive persons (positive skin test, positive history) and 40 latent pollen-allergic persons (positive skin test, but no conjunctival or nasal manifestations during the flowering season) were given intranasally increasing concentrations of pollen extract until a reaction occurred (itching, sneezing, running and blocked nose). 7.4% of the patients reacted to 0.001% w/v of the pollen extract, 56.5% reacted to 0.01, and 36% to a dose of 0.1% w/v. The corresponding figures for persons with latent allergy were 0.7, 5 and 30%; 62.5% reacted to 1.0 or 2.5% w/v of the extract. There was a relatively sharp division between doses of 0.1 and 1.0% w/v: persons who responded only to 1% concentrations invariably belonged to the group of latent allergy. 35 persons with positive skin tests to dust mites were divided into 3 groups: those with either a positive or a negative history of dust allergy and those concentrations of dust mite extracts (0.012, 0.12 and 1.2% w/v). Patients were considered to have latent allergy if they were sensitive to dust mite but were free from symptoms; or if they were sensitive to dust mite and pollen, but were symptom-free outside the flowering season. Although the group reacted slightly more often only to concentrations of 1.2%, the nasal response occurred on the whole with the same frequency with all three concentrations. In contrast to pollen allergy a positive intranasal dust mite test is not necessarily clinically relevant. Provocation tests are, therefore, not an infallible means to ascertain the presence of clinically relevant allergy."}
{"id": "PMID:461310", "title": "[Immunotherapy with BCG and mycobacterial fractions and its use in bronchogenic carcinoma (author's transl)].", "content": "Animal experiments at the Nat. Cancer Inst. have established that only intra-tumorous application of BCG vaccine has a lasting immunotherapeutic effect on transplantable tumours. However, clinical observations have proved that BCG immunotherapy by the intra-tumorous route increases the incidence of complications, such as allergic reactions and generalized spread of BCG, to an unacceptable level. Ribi has produced a vaccine from mycobacterial fractions which is low in protein and which, as proved in clinical pilot studies in cases of melanoma and cancer of the breast, is so well tolerated that it can be injected into the tumour. Since bronchogenic carcinoma is in the majority of cases inoperable, but, on the other hand, can be reached directly via the bronchoscope or the perthoracic route a pilot study with the Ribi vaccine was started in patients with lung cancer. The preliminary results are reported.", "contents": "[Immunotherapy with BCG and mycobacterial fractions and its use in bronchogenic carcinoma (author's transl)]. Animal experiments at the Nat. Cancer Inst. have established that only intra-tumorous application of BCG vaccine has a lasting immunotherapeutic effect on transplantable tumours. However, clinical observations have proved that BCG immunotherapy by the intra-tumorous route increases the incidence of complications, such as allergic reactions and generalized spread of BCG, to an unacceptable level. Ribi has produced a vaccine from mycobacterial fractions which is low in protein and which, as proved in clinical pilot studies in cases of melanoma and cancer of the breast, is so well tolerated that it can be injected into the tumour. Since bronchogenic carcinoma is in the majority of cases inoperable, but, on the other hand, can be reached directly via the bronchoscope or the perthoracic route a pilot study with the Ribi vaccine was started in patients with lung cancer. The preliminary results are reported."}
{"id": "PMID:461311", "title": "[Immunological studies and immunotherapeutic possibilities in inoperable bronchogenic carcinoma (author's transl)].", "content": "By establishing an immune profile combined with detailed clinical observation an attempt was made to assess the value of various therapeutic measures, especially immunological methods, in the treatment of inoperable bronchogenic carcinoma. The results indicated that in advanced cases specific immunological measures or stimulation of the immune mechanism only play a supportive role. Toxic side-effects or tumour enhancement were absent.", "contents": "[Immunological studies and immunotherapeutic possibilities in inoperable bronchogenic carcinoma (author's transl)]. By establishing an immune profile combined with detailed clinical observation an attempt was made to assess the value of various therapeutic measures, especially immunological methods, in the treatment of inoperable bronchogenic carcinoma. The results indicated that in advanced cases specific immunological measures or stimulation of the immune mechanism only play a supportive role. Toxic side-effects or tumour enhancement were absent."}
{"id": "PMID:461312", "title": "[Architectonics of the thorax (author's transl)].", "content": "Blunt traumata cause injury to the thorax and intrathoracic organs by deforming the chest wall and the enclosed structures. Neither examination of the dead person and re-enactment of the accident nor experimental studies on the living or dead person can fully elucidate the course of events. A more satisfactory approach is to use biomechanic models to simulate the structure of the thorax and the type of deformation it undergoes when subjected to various traumata. Such an elasto-static analysis presupposes knowledge of (1) the global geometry of the thorax; (2) the cross sectional geometry of the individual components; (3) the mechanical properties of the material; (4) the force of the impact; (5) concomitant conditions. The results obtained with the models are reviewed. They make it possible to predict the deformation of the thorax in response to the force of the impact, the location and type of fracture of the ribs and the deformation of the intrathoracic organs.", "contents": "[Architectonics of the thorax (author's transl)]. Blunt traumata cause injury to the thorax and intrathoracic organs by deforming the chest wall and the enclosed structures. Neither examination of the dead person and re-enactment of the accident nor experimental studies on the living or dead person can fully elucidate the course of events. A more satisfactory approach is to use biomechanic models to simulate the structure of the thorax and the type of deformation it undergoes when subjected to various traumata. Such an elasto-static analysis presupposes knowledge of (1) the global geometry of the thorax; (2) the cross sectional geometry of the individual components; (3) the mechanical properties of the material; (4) the force of the impact; (5) concomitant conditions. The results obtained with the models are reviewed. They make it possible to predict the deformation of the thorax in response to the force of the impact, the location and type of fracture of the ribs and the deformation of the intrathoracic organs."}
{"id": "PMID:461313", "title": "[The physiology of intrathoracic pressure changes (author's transl)].", "content": "The pleural cavity, because of the thin film of fluid between the pleural layers, acts like a capillary space within which capillary forces operate. It is due to these forces that the lungs follow the respiratory movements of the thoracic cage and diaphragm and are kept expanded within the thorax. \"Subatmospheric\" pressure in the pleural cavity develops only if the capillary space is opened by injury to one of the pleural layers, thus allowing the elastic pull of the thoracic cage and lungs to act on the pleural cavity. The subatmospheric pressure registered by the exploratory needle is, in fact, non-existent in the normal chest. It owes its existence to the introduction of the needle which causes separation of the pleural layers and the introduction of a small quantity of air. The pulling and stretching forces exerted by the thorax and lungs on the air bubble then become measurable as subatmospheric pressure. The same forces operate during the respiratory movements and induce changes in volume and pressure in all air or fluid containing spaces. The result is a delicately regulated consonance of aire movement and blood distribution. Simplified graphs illustrate the interconnections.", "contents": "[The physiology of intrathoracic pressure changes (author's transl)]. The pleural cavity, because of the thin film of fluid between the pleural layers, acts like a capillary space within which capillary forces operate. It is due to these forces that the lungs follow the respiratory movements of the thoracic cage and diaphragm and are kept expanded within the thorax. \"Subatmospheric\" pressure in the pleural cavity develops only if the capillary space is opened by injury to one of the pleural layers, thus allowing the elastic pull of the thoracic cage and lungs to act on the pleural cavity. The subatmospheric pressure registered by the exploratory needle is, in fact, non-existent in the normal chest. It owes its existence to the introduction of the needle which causes separation of the pleural layers and the introduction of a small quantity of air. The pulling and stretching forces exerted by the thorax and lungs on the air bubble then become measurable as subatmospheric pressure. The same forces operate during the respiratory movements and induce changes in volume and pressure in all air or fluid containing spaces. The result is a delicately regulated consonance of aire movement and blood distribution. Simplified graphs illustrate the interconnections."}
{"id": "PMID:461314", "title": "[Diagnostic problems in chest injuries (angiography) (author's transl)].", "content": "Roentgenography is the simplest and most reliable means to arrive at the diagnosis of chest injury. General roentgenograms are difficult to interpret as they tend to be technically imperfect. Fractures, emphysema, pneumothorax, accumulation of fluid can usually be ascertained directly; but the traumatic origin of changes in the pulmonary parenchyma or of an enlarged heart shadow cannot be reliably deduced from the X-ray appearance. It may provide some differential-diagnostic information but the correct interpretation of the findings depends on further observation. In 6-7% of severe chest trauma with vascular injuries and rupture of the diaphragm angiography is indicated. The evidence to be obtained from chest radiography should not be overestimated: fractures of ribs are sometimes overlocked, even by the expert; parenchymatous lesions may manifest themselves as shadows but their nature remains obscure until they have been related to the clinical and subsequent radiological findings. The same applies to rupture of the diaphragm, bronchi or vessels, if only the immediate posttraumatic roentgenographs are examined. A tent-shaped heart shadow is considered characteristic of the presence of fluid in the pericardium; this is valid only for chronic hydropericardium, but not for the potentially fatal cardiac tamponade; if the pericardium has lost its elasticity a haemorrhage of not more than 150 ml may prove fatal. Nor does the roentgenogram provide information about pulmonary function. Especially in cases of pulmonary shock minor changes in the chest roentgenogram may give a false sense of security when, in fact, blood gas analyses show that a life-endangering situation has developed. The radiologist who is aware of the limititations of the method will derive maximum diagnostic benefit from a chest angiography. No other method is capable of supplying information of such great importance in such a short time.", "contents": "[Diagnostic problems in chest injuries (angiography) (author's transl)]. Roentgenography is the simplest and most reliable means to arrive at the diagnosis of chest injury. General roentgenograms are difficult to interpret as they tend to be technically imperfect. Fractures, emphysema, pneumothorax, accumulation of fluid can usually be ascertained directly; but the traumatic origin of changes in the pulmonary parenchyma or of an enlarged heart shadow cannot be reliably deduced from the X-ray appearance. It may provide some differential-diagnostic information but the correct interpretation of the findings depends on further observation. In 6-7% of severe chest trauma with vascular injuries and rupture of the diaphragm angiography is indicated. The evidence to be obtained from chest radiography should not be overestimated: fractures of ribs are sometimes overlocked, even by the expert; parenchymatous lesions may manifest themselves as shadows but their nature remains obscure until they have been related to the clinical and subsequent radiological findings. The same applies to rupture of the diaphragm, bronchi or vessels, if only the immediate posttraumatic roentgenographs are examined. A tent-shaped heart shadow is considered characteristic of the presence of fluid in the pericardium; this is valid only for chronic hydropericardium, but not for the potentially fatal cardiac tamponade; if the pericardium has lost its elasticity a haemorrhage of not more than 150 ml may prove fatal. Nor does the roentgenogram provide information about pulmonary function. Especially in cases of pulmonary shock minor changes in the chest roentgenogram may give a false sense of security when, in fact, blood gas analyses show that a life-endangering situation has developed. The radiologist who is aware of the limititations of the method will derive maximum diagnostic benefit from a chest angiography. No other method is capable of supplying information of such great importance in such a short time."}
{"id": "PMID:461315", "title": "[Emergency treatment in chest trauma (author's transl)].", "content": "The measures taken by the emergency physician can in many cases decisively affect the subsequent course of a chest trauma. In cases where the injured person has to be extricated from collapsed structures the medical officer has to decide on the mode and sequence of the rescue operation, prevent further damage and initiate shock therapy, intubation and artificial ventilation while the rescue operation is in progress. Emergency treatment also includes infusion therapy, if necessary via a central venous catheter; intubation, suction drainage and artificial ventilation; evacuation of fluid from the pleural cavity, mediastinum and pericardium combined with electrodiagnosis (ECG) and electrotherapy (defibrillation) if necessary. By closely observing the reaction and condition of the patient during transport to hospital he can provide important information regarding further treatment, especially in polytraumatized persons. Close collaboration between the trained rescue teams of the Fire Service, the emergency medical officer and the hospital staff has succeeded in reducing the mortality rate of combined chest injuries to 13%. 87% of cases who had received emergency treatment were subsequently admitted to hospital.", "contents": "[Emergency treatment in chest trauma (author's transl)]. The measures taken by the emergency physician can in many cases decisively affect the subsequent course of a chest trauma. In cases where the injured person has to be extricated from collapsed structures the medical officer has to decide on the mode and sequence of the rescue operation, prevent further damage and initiate shock therapy, intubation and artificial ventilation while the rescue operation is in progress. Emergency treatment also includes infusion therapy, if necessary via a central venous catheter; intubation, suction drainage and artificial ventilation; evacuation of fluid from the pleural cavity, mediastinum and pericardium combined with electrodiagnosis (ECG) and electrotherapy (defibrillation) if necessary. By closely observing the reaction and condition of the patient during transport to hospital he can provide important information regarding further treatment, especially in polytraumatized persons. Close collaboration between the trained rescue teams of the Fire Service, the emergency medical officer and the hospital staff has succeeded in reducing the mortality rate of combined chest injuries to 13%. 87% of cases who had received emergency treatment were subsequently admitted to hospital."}
{"id": "PMID:461316", "title": "[Chest injuries (author's transl)].", "content": "The mortality rate of chest injuries sustained during work or in road accidents stands now at 15-20%. The considerable force of the impact in road accidents is, in 60-80% of the cases, responsible for chest injuries which involve not only the chest wall but also the lungs. The extent and course of the lung damage must be assessed by repeated X-ray examinations, blood gas analyses and clinical observations. The decision to intubate and apply artificial ventilation should be made at an early stage. Fracture of a single rib needs only pain killers. If a rib is broken in several places or if several ribs are fractured instability of the chest wall is apt to develop in 15-20% of the cases. It manifests itself in paradoxical breathing and ensuing increase in the dead space. These cases require prompt \"internal pneumatic splinting\" in the form of positive pressure respiration and intubation. In recent years surgical stabilization of the chest wall has regained favour. Plate osteosynthesis, screw-less rib plates, self-gripping steel plates and steel splints are being used (Brunner, Hofmeister, Koncz). Primary osteosynthetic stabilization of the chest wall is indicated only if artificial ventilation has proved inadequate and there are other reasons for performing a thoracotomy. In these circumstances surgical intervention ensures that prolonged artificial ventilation and its attendent risks and complications and the demands made on the nursing staff are reduced to a minimum.", "contents": "[Chest injuries (author's transl)]. The mortality rate of chest injuries sustained during work or in road accidents stands now at 15-20%. The considerable force of the impact in road accidents is, in 60-80% of the cases, responsible for chest injuries which involve not only the chest wall but also the lungs. The extent and course of the lung damage must be assessed by repeated X-ray examinations, blood gas analyses and clinical observations. The decision to intubate and apply artificial ventilation should be made at an early stage. Fracture of a single rib needs only pain killers. If a rib is broken in several places or if several ribs are fractured instability of the chest wall is apt to develop in 15-20% of the cases. It manifests itself in paradoxical breathing and ensuing increase in the dead space. These cases require prompt \"internal pneumatic splinting\" in the form of positive pressure respiration and intubation. In recent years surgical stabilization of the chest wall has regained favour. Plate osteosynthesis, screw-less rib plates, self-gripping steel plates and steel splints are being used (Brunner, Hofmeister, Koncz). Primary osteosynthetic stabilization of the chest wall is indicated only if artificial ventilation has proved inadequate and there are other reasons for performing a thoracotomy. In these circumstances surgical intervention ensures that prolonged artificial ventilation and its attendent risks and complications and the demands made on the nursing staff are reduced to a minimum."}
{"id": "PMID:461317", "title": "[Open and closed chest trauma (author's transl)].", "content": "The observations made in 1,023 cases of chest trauma during 1947-1976 inclusive are analysed. According to the extent of the injuries they were classified as mild (group 1), moderate (group 2) and severe (group 3). The clinical picture was dominated by other than chest injuries in 45% of patients in group 1, in 66% in group 2 and in 51% in group 3. Injuries to the intrathoracic organs were seen in only 34 patients. Total mortality of all chest injuries was 12%; in group 3 it was 62%. Severe respiratory insufficiency was the direct cause of death in 25% of persons in group 3.", "contents": "[Open and closed chest trauma (author's transl)]. The observations made in 1,023 cases of chest trauma during 1947-1976 inclusive are analysed. According to the extent of the injuries they were classified as mild (group 1), moderate (group 2) and severe (group 3). The clinical picture was dominated by other than chest injuries in 45% of patients in group 1, in 66% in group 2 and in 51% in group 3. Injuries to the intrathoracic organs were seen in only 34 patients. Total mortality of all chest injuries was 12%; in group 3 it was 62%. Severe respiratory insufficiency was the direct cause of death in 25% of persons in group 3."}
{"id": "PMID:461318", "title": "[Surgical aspects of open and closed chest injuries (author's transl)].", "content": "263 persons with chest injuries were treated over a period of 16 years. 47 had performating chest injuries, in the remaining cases it was a blunt trauma. Prompt recognition of a life-endangering situation (cardiac tamponade, tension pneumothorax, mediastinal emphysema, massive haemothorax) is essential; radiological and laboratory diagnostic methods play a secondary role. The most effective emergency treatment is intercostal continuous suction; in many cases it is the only one needed. A haemothorax must be evacuated completely because of the risk of complications and fibrin formation. Thoracotomy is rarely, and surgical removal of lung tissue hardly ever, indicated. 30 persons needed emergency thoracotomy. Accompanying intraabdominal injuries, mostly rupture of the diaphragm and spleen, were observed in 45 patients. Late sequels were lung abscesses, posttraumatic cysts, pleural empyema and adhesions, atelectases. Decortication, if indicated, should be performed at an early stage.", "contents": "[Surgical aspects of open and closed chest injuries (author's transl)]. 263 persons with chest injuries were treated over a period of 16 years. 47 had performating chest injuries, in the remaining cases it was a blunt trauma. Prompt recognition of a life-endangering situation (cardiac tamponade, tension pneumothorax, mediastinal emphysema, massive haemothorax) is essential; radiological and laboratory diagnostic methods play a secondary role. The most effective emergency treatment is intercostal continuous suction; in many cases it is the only one needed. A haemothorax must be evacuated completely because of the risk of complications and fibrin formation. Thoracotomy is rarely, and surgical removal of lung tissue hardly ever, indicated. 30 persons needed emergency thoracotomy. Accompanying intraabdominal injuries, mostly rupture of the diaphragm and spleen, were observed in 45 patients. Late sequels were lung abscesses, posttraumatic cysts, pleural empyema and adhesions, atelectases. Decortication, if indicated, should be performed at an early stage."}
{"id": "PMID:461319", "title": "[Indications and methods for the surgical stabilization of an unstable thorax caused by multiple fractures of the ribs (author's transl)].", "content": "Every year 8,000 persons involved in road accidents die of irreversible respiratory insufficiency caused by chest injuries. Fractures of the ribs extendig over a number of ribs are present in 70% of the cases and in 15% of them they are accompanied by instability of the thorax and \"paradoxical\" respiration. Treatment of the instability by \"internal pneumatic splinting\" (Avery) and by surgical measures is reviewed. Indications for the latter are: (1) open fractures of the chest wall; (2) instability of the thorax combined with intrathoracic injuries; (3) instability of the chest wall with severe therapy-resistant respiratory insufficiency. In a series of animal experiments unilateral instability of the chest wall was induced by multiple rib fractures. By measuring oscillatory resistance before and after stabilization of chest wall the effects of the latter on the impaired lung function could be demonstrated. The results of the experiments indicate that with this type of surgical treatment mortality from combined thoracic injuries with paradoxical breathing can be reduced.", "contents": "[Indications and methods for the surgical stabilization of an unstable thorax caused by multiple fractures of the ribs (author's transl)]. Every year 8,000 persons involved in road accidents die of irreversible respiratory insufficiency caused by chest injuries. Fractures of the ribs extendig over a number of ribs are present in 70% of the cases and in 15% of them they are accompanied by instability of the thorax and \"paradoxical\" respiration. Treatment of the instability by \"internal pneumatic splinting\" (Avery) and by surgical measures is reviewed. Indications for the latter are: (1) open fractures of the chest wall; (2) instability of the thorax combined with intrathoracic injuries; (3) instability of the chest wall with severe therapy-resistant respiratory insufficiency. In a series of animal experiments unilateral instability of the chest wall was induced by multiple rib fractures. By measuring oscillatory resistance before and after stabilization of chest wall the effects of the latter on the impaired lung function could be demonstrated. The results of the experiments indicate that with this type of surgical treatment mortality from combined thoracic injuries with paradoxical breathing can be reduced."}
{"id": "PMID:461320", "title": "[Stabilization of the thoracic wall (author's transl)].", "content": "The improvement of mechanical respiration by surgical stabilization of the chest wall is an important measure to reduce disadvantages and complications of long time respiratory treatment. Indications are: (1) Chest wall instability with indication for emergency thoracotomy; (2) Respiratory insufficiency mainly caused by chest wall instability; (3) Respiratory insufficiency due to severe laceration of intrathoracical organs with additional chest wall instability as an obstacle for sufficient respirator treatment.", "contents": "[Stabilization of the thoracic wall (author's transl)]. The improvement of mechanical respiration by surgical stabilization of the chest wall is an important measure to reduce disadvantages and complications of long time respiratory treatment. Indications are: (1) Chest wall instability with indication for emergency thoracotomy; (2) Respiratory insufficiency mainly caused by chest wall instability; (3) Respiratory insufficiency due to severe laceration of intrathoracical organs with additional chest wall instability as an obstacle for sufficient respirator treatment."}
{"id": "PMID:461322", "title": "[Injury to the lung parenchyma (author's transl)].", "content": "Injuries involving only the lungs are relatively rare; generally they are a combination of thoracic and pulmonary trauma. As a result of the increased incidence of severe polytraumata combined injuries have become more frequent. Isolated thoraco-pulmonary injuries by stabbing or a bullet are comparatively rare. Various types of lung injuries are described. During 1970-1976 388 persons with various injuries involving the chest were admitted to the Surgical Unit of the University Hospital, D\u00fcsseldorf. In 155 of them (40%) the lungs were injured; in only 49 was the trauma confined to the thorax and lungs. Over-all mortality was 60%. The case material was classified as (1) isolated lung injuries without damage to the thorax; (2) combined injuries to the chest wall and lungs. Combined injuries predominated in the proportion of 136:19 cases. Polytraumatised persons predominated in both groups (71 and 98% respectively). The clinical features and treatment of the various types of injuries are reviewed.", "contents": "[Injury to the lung parenchyma (author's transl)]. Injuries involving only the lungs are relatively rare; generally they are a combination of thoracic and pulmonary trauma. As a result of the increased incidence of severe polytraumata combined injuries have become more frequent. Isolated thoraco-pulmonary injuries by stabbing or a bullet are comparatively rare. Various types of lung injuries are described. During 1970-1976 388 persons with various injuries involving the chest were admitted to the Surgical Unit of the University Hospital, D\u00fcsseldorf. In 155 of them (40%) the lungs were injured; in only 49 was the trauma confined to the thorax and lungs. Over-all mortality was 60%. The case material was classified as (1) isolated lung injuries without damage to the thorax; (2) combined injuries to the chest wall and lungs. Combined injuries predominated in the proportion of 136:19 cases. Polytraumatised persons predominated in both groups (71 and 98% respectively). The clinical features and treatment of the various types of injuries are reviewed."}
{"id": "PMID:461323", "title": "[Clinical classification of the severity of lung injuries (author's transl)].", "content": "The observations made in 651 cases of traumatic lung injuries suggested the advisability of dividing these injuries into three groups according to the severity of the clinical picture. The criterion was the degree of impairment of respiratory function as assessed by repeated gas analyses. By relating function to severity of the clinical picture it was possible not only to institute the appropriate therapeutic measures but also to obtain information regarding the prognosis. Lung injuries in group 2 and 3 require prompt transfer to an intensive care unit with experience in chest surgery before irreversible damage has set in.", "contents": "[Clinical classification of the severity of lung injuries (author's transl)]. The observations made in 651 cases of traumatic lung injuries suggested the advisability of dividing these injuries into three groups according to the severity of the clinical picture. The criterion was the degree of impairment of respiratory function as assessed by repeated gas analyses. By relating function to severity of the clinical picture it was possible not only to institute the appropriate therapeutic measures but also to obtain information regarding the prognosis. Lung injuries in group 2 and 3 require prompt transfer to an intensive care unit with experience in chest surgery before irreversible damage has set in."}
{"id": "PMID:461324", "title": "[Trachea and main bronchi in thoracal trauma (author's transl)].", "content": "On the whole, only few lesions of the trachea and main bronchi are amenable to treatment. The ideal treatment is that which is applied immediately in so far as successful management of the acute shock has been achieved. Reluctance to effect mechanical stabilisation of the thoracic wall by means of wires or metal plates, has been fortunately abandoned. The increased application of fibre bronchoscopy by surgical intensive-care units, or regular consultation of a bronchologist or thoracic surgeon, has improved chances of earlier detection of any serious lesion of the main respiratory tract. Lesions of the parenchyma are important and significant only to the extent as they can become serious or insurmountable obstacles during immediate dressing and final surgical treatment if they are present side by side with a lesion of the trachea or of the main bronchi.", "contents": "[Trachea and main bronchi in thoracal trauma (author's transl)]. On the whole, only few lesions of the trachea and main bronchi are amenable to treatment. The ideal treatment is that which is applied immediately in so far as successful management of the acute shock has been achieved. Reluctance to effect mechanical stabilisation of the thoracic wall by means of wires or metal plates, has been fortunately abandoned. The increased application of fibre bronchoscopy by surgical intensive-care units, or regular consultation of a bronchologist or thoracic surgeon, has improved chances of earlier detection of any serious lesion of the main respiratory tract. Lesions of the parenchyma are important and significant only to the extent as they can become serious or insurmountable obstacles during immediate dressing and final surgical treatment if they are present side by side with a lesion of the trachea or of the main bronchi."}
{"id": "PMID:461325", "title": "[Tracheal and bronchial ruptures (author's transl)].", "content": "Rupture of the major airways is rare. Open injuries usually involve the trachea; injuries to the airways in closed chest trauma are in 80-90% of the cases located at the bifurcation or the main bronchus. Rupture of the bronchial system may cause an immediate threat to life, but may also produce few symptoms. The possibility of such an injury should always be taken into consideration, even if at first the clinical signs only point to damage of the lung parenchyma. Intubation and continuous suction drainage are life-saving emergency measures to be followed as soon as possible by bronchoscopy to establish the diagnosis. Treatment is by prompt suture or anastomosis. Failure to diagnose rupture of a bronchus results very soon in stenosis and, later on, in damage to the lungs and jeopardizes the success of subsequent reconstructive surgery. The repair of extended stenoses presents particularly difficult technical problems. Alloplastic grafts may be the answer. During 1961-1978 7 persons with injuries to the trachea and bronchi were treated at the Department of Surgery, Giessen. 3 persons were saved by immediately performing an anastomosis.", "contents": "[Tracheal and bronchial ruptures (author's transl)]. Rupture of the major airways is rare. Open injuries usually involve the trachea; injuries to the airways in closed chest trauma are in 80-90% of the cases located at the bifurcation or the main bronchus. Rupture of the bronchial system may cause an immediate threat to life, but may also produce few symptoms. The possibility of such an injury should always be taken into consideration, even if at first the clinical signs only point to damage of the lung parenchyma. Intubation and continuous suction drainage are life-saving emergency measures to be followed as soon as possible by bronchoscopy to establish the diagnosis. Treatment is by prompt suture or anastomosis. Failure to diagnose rupture of a bronchus results very soon in stenosis and, later on, in damage to the lungs and jeopardizes the success of subsequent reconstructive surgery. The repair of extended stenoses presents particularly difficult technical problems. Alloplastic grafts may be the answer. During 1961-1978 7 persons with injuries to the trachea and bronchi were treated at the Department of Surgery, Giessen. 3 persons were saved by immediately performing an anastomosis."}
{"id": "PMID:461326", "title": "[Indications for the correction of tracheal stenosis caused by prolonged artificial ventilation of polytraumatized persons (author's transl)].", "content": "Pronounced, short-length narrowing of the trachea combined with dyspnoea at rest is an absolute indication for partial resection. For stenoses of moderate degree the indications for surgical intervention are as yet not clearly defined. They depend not only on the clinical, roentgenological and tracheobronchoscopic findings, but also on the results of lung function tests. Changes in respiratory function become noticeable at reduction of under 30% of the tracheal diameter. Experiments with artificially induced stenosis have shown an exponential rise in flow resistance when the diameter of the trachea was narrowed to below 8 millimetre. observations made in patients have proved that the experimental results also apply clinically. The indications for surgery of a tracheal stenosis should be made dependent on the results of the determinations of flow resistance, whole body plethysmography and expiratory and inspiratory volume/1 sec. Flow resistance exceeding 5 cm H2O/1 sec, equivalent to narrowing to under 8 mm in diameter, is considered to be an indication for partial resection.", "contents": "[Indications for the correction of tracheal stenosis caused by prolonged artificial ventilation of polytraumatized persons (author's transl)]. Pronounced, short-length narrowing of the trachea combined with dyspnoea at rest is an absolute indication for partial resection. For stenoses of moderate degree the indications for surgical intervention are as yet not clearly defined. They depend not only on the clinical, roentgenological and tracheobronchoscopic findings, but also on the results of lung function tests. Changes in respiratory function become noticeable at reduction of under 30% of the tracheal diameter. Experiments with artificially induced stenosis have shown an exponential rise in flow resistance when the diameter of the trachea was narrowed to below 8 millimetre. observations made in patients have proved that the experimental results also apply clinically. The indications for surgery of a tracheal stenosis should be made dependent on the results of the determinations of flow resistance, whole body plethysmography and expiratory and inspiratory volume/1 sec. Flow resistance exceeding 5 cm H2O/1 sec, equivalent to narrowing to under 8 mm in diameter, is considered to be an indication for partial resection."}
{"id": "PMID:461327", "title": "[Diagnosis and treatment of tracheo-oesophageal fistula (author's transl)].", "content": "Three cases of tracheo-oesophageal fistula as an extremely rare complication of blunt chest trauma are described in detail. 32 similar cases reported in the medical literature are analysed and the pathogenesis of this type of injury is discussed.", "contents": "[Diagnosis and treatment of tracheo-oesophageal fistula (author's transl)]. Three cases of tracheo-oesophageal fistula as an extremely rare complication of blunt chest trauma are described in detail. 32 similar cases reported in the medical literature are analysed and the pathogenesis of this type of injury is discussed."}
{"id": "PMID:461328", "title": "[Injury to the diaphragm (author's transl)].", "content": "A distinction is made between acute injury to the diaphragm (18 cases) and late sequels of such lesions (enterothorax, 9 cases). The acute trauma is often not felt as such and it is only after some time that its consequences manifest themselves. The effect of a trauma on the diaphragm depends on whether the former acts on the abdomen or thorax. Surgical repair of the damaged diaphragm is nearly always feasible regardless of whether the operation is performed at an early or late stage of the injury. In only two cases (since 1963) was it necessary to resort to heteroplastic material, viz.: lyophilized dura mater. The possibility that viscera displaced into the thorax are also injured must be taken into consideration. Rupture of the diaphragm involved the left side in 95% and the right half in 5% of the cases (protection provided by the liver).", "contents": "[Injury to the diaphragm (author's transl)]. A distinction is made between acute injury to the diaphragm (18 cases) and late sequels of such lesions (enterothorax, 9 cases). The acute trauma is often not felt as such and it is only after some time that its consequences manifest themselves. The effect of a trauma on the diaphragm depends on whether the former acts on the abdomen or thorax. Surgical repair of the damaged diaphragm is nearly always feasible regardless of whether the operation is performed at an early or late stage of the injury. In only two cases (since 1963) was it necessary to resort to heteroplastic material, viz.: lyophilized dura mater. The possibility that viscera displaced into the thorax are also injured must be taken into consideration. Rupture of the diaphragm involved the left side in 95% and the right half in 5% of the cases (protection provided by the liver)."}
{"id": "PMID:461329", "title": "[Diagnosis and treatment of blunt injury to the diaphragm (author's transl)].", "content": "Blunt injuries to the diaphragm are rare. They usually occur in combination with injuries to other structures (craniocerebral trauma, fracture of the pelvis, rupture of the spleen or liver). Regarding the diagnosis, knowledge of the mechanism of the accident may point to the possibility of a ruptured diaphragm, and so may auscultation and percussion of the chest. Examination of the gastrointestinal tract and contrast enema may show up displacement of abdominal viscera into the left pleural cavity. In cases of rupture of the right diaphragm coeliacography and scintillation scanning are indicated. As regards treatment: every rupture of the diaphragm needs surgical repair. If prolapse of viscera has been established, prompt intervention is necessary because of the risk of acute incarceration. Asymptomatic prolapse causing only minor complaints should also be rectified to obviate complications. The best approach for both early and late repair of the diaphragm is via the thorax.", "contents": "[Diagnosis and treatment of blunt injury to the diaphragm (author's transl)]. Blunt injuries to the diaphragm are rare. They usually occur in combination with injuries to other structures (craniocerebral trauma, fracture of the pelvis, rupture of the spleen or liver). Regarding the diagnosis, knowledge of the mechanism of the accident may point to the possibility of a ruptured diaphragm, and so may auscultation and percussion of the chest. Examination of the gastrointestinal tract and contrast enema may show up displacement of abdominal viscera into the left pleural cavity. In cases of rupture of the right diaphragm coeliacography and scintillation scanning are indicated. As regards treatment: every rupture of the diaphragm needs surgical repair. If prolapse of viscera has been established, prompt intervention is necessary because of the risk of acute incarceration. Asymptomatic prolapse causing only minor complaints should also be rectified to obviate complications. The best approach for both early and late repair of the diaphragm is via the thorax."}
{"id": "PMID:461330", "title": "[Traumatic rupture of the diaphragm (author's transl)].", "content": "Traumatic rupture of the diaphragm is diagnosed during the acute stage in only 50% of the cases. The reasons for this failure are that in many of these cases clinical symptoms of damage to abdominal organs or of injuries to the limbs predominate. Traumatic rupture of the diaphragm is usually the result of an indirect trauma. Clinical signs of extensive prolapse of viscera into the thoracic cavity are: dyspnoea, tachycardia, cyanosis or intestinal sounds heard over the thorax during auscultation. Once the diagnosis has been established surgical repair should follow. During the acute stage laparotomy is preferable; thoracotomy is indicated during the chronic stage. Post-operative symptoms are: dyspnoea during exercise, pain in the affected half of the chest and roentgeno-kymographically demonstrable restrictions of movement in the ruptured side of the diaphragm.", "contents": "[Traumatic rupture of the diaphragm (author's transl)]. Traumatic rupture of the diaphragm is diagnosed during the acute stage in only 50% of the cases. The reasons for this failure are that in many of these cases clinical symptoms of damage to abdominal organs or of injuries to the limbs predominate. Traumatic rupture of the diaphragm is usually the result of an indirect trauma. Clinical signs of extensive prolapse of viscera into the thoracic cavity are: dyspnoea, tachycardia, cyanosis or intestinal sounds heard over the thorax during auscultation. Once the diagnosis has been established surgical repair should follow. During the acute stage laparotomy is preferable; thoracotomy is indicated during the chronic stage. Post-operative symptoms are: dyspnoea during exercise, pain in the affected half of the chest and roentgeno-kymographically demonstrable restrictions of movement in the ruptured side of the diaphragm."}
{"id": "PMID:461331", "title": "[Unrecognized traumatic rupture of the diaphragm (author's transl)].", "content": "20 cases of rupture of the diaphragm (one due to direct trauma, the other 19 due to indirect trauma) which were not recognized at the time of the accident are described. The reasons for the delay in diagnosing the true nature of the condition and the misleading complaints caused by the unrecognized injury are reviewed. Essential diagnostic measures are discussed and guide-lines are suggested to ensure the early diagnosis of rupture of the diaphragm.", "contents": "[Unrecognized traumatic rupture of the diaphragm (author's transl)]. 20 cases of rupture of the diaphragm (one due to direct trauma, the other 19 due to indirect trauma) which were not recognized at the time of the accident are described. The reasons for the delay in diagnosing the true nature of the condition and the misleading complaints caused by the unrecognized injury are reviewed. Essential diagnostic measures are discussed and guide-lines are suggested to ensure the early diagnosis of rupture of the diaphragm."}
{"id": "PMID:461332", "title": "[Injuries to the diaphragm (author's transl)].", "content": "A traumatic rupture of the diaphragm is nearly always accompanied by injuries to the intrathoracic and abdominal structures. As the symptoms arising from these injured organs generally predominate, the presence of a ruptured diaphragm is apt to remain unrecognized or to be diagnosed at a very late stage. If the diagnosis is made immediately after the accident surgical repair should follow promptly; if the rupture is of long standing (traumatic hernia) surgery should preferably be performed during a symptom-free interval. An analysis of the case material showed that the left diaphragm was more often involved than the right one, an observation that tallies with the published reports. The transthoracic approach provides a clearer view of the operating area and was, therefore, chosen. The post-operative results were satisfactory; mortality rate was nil.", "contents": "[Injuries to the diaphragm (author's transl)]. A traumatic rupture of the diaphragm is nearly always accompanied by injuries to the intrathoracic and abdominal structures. As the symptoms arising from these injured organs generally predominate, the presence of a ruptured diaphragm is apt to remain unrecognized or to be diagnosed at a very late stage. If the diagnosis is made immediately after the accident surgical repair should follow promptly; if the rupture is of long standing (traumatic hernia) surgery should preferably be performed during a symptom-free interval. An analysis of the case material showed that the left diaphragm was more often involved than the right one, an observation that tallies with the published reports. The transthoracic approach provides a clearer view of the operating area and was, therefore, chosen. The post-operative results were satisfactory; mortality rate was nil."}
{"id": "PMID:461333", "title": "[Diagnosis and treatment of acute injuries to the heart and intrathoracic vessels (author's transl)].", "content": "The massive blood loss that accompanies penetrating chest wounds involving the heart or intrathoracic vessels generally leaves no option but to act immediately. Blunt chest trauma in combination with other injuries makes particularly stringent demands on diagnostic and therapeutic skills. Most injuries to the intrathoracic aorta are in the form of intiially closed tears of the descending part; but rupture is an ever-present threat. The symptoms are: enlargement of the mediastinum in the chest roentgenogram, differences in blood pressure between the upper and lower half of the body and haemothorax. Whenever possible aortography should be performed to locate the site of the rupture. The therapeutic measures are determined by the extent and severity of the various injuries.", "contents": "[Diagnosis and treatment of acute injuries to the heart and intrathoracic vessels (author's transl)]. The massive blood loss that accompanies penetrating chest wounds involving the heart or intrathoracic vessels generally leaves no option but to act immediately. Blunt chest trauma in combination with other injuries makes particularly stringent demands on diagnostic and therapeutic skills. Most injuries to the intrathoracic aorta are in the form of intiially closed tears of the descending part; but rupture is an ever-present threat. The symptoms are: enlargement of the mediastinum in the chest roentgenogram, differences in blood pressure between the upper and lower half of the body and haemothorax. Whenever possible aortography should be performed to locate the site of the rupture. The therapeutic measures are determined by the extent and severity of the various injuries."}
{"id": "PMID:461334", "title": "[Diagnosis and treatment of post-traumatic aneurysm of the thoracic aorta (author's transl)].", "content": "A post-traumatic false aneurysm of the thoracic aorta is generally the result of a severe closed chest trauma in which deceleration and compression mechanisms operate on the aortic isthmus. Early diagnosis is difficult except where the lesion progresses to rupture. A not inconsiderable number of incomplete and initially closed tears in the wall of the aortic isthmus escape detection and in due course lead to dilatation of the vessel and false aneurysm. The condition is diagnosed, often accidentally, during aortography. The indications for surgical intervention are given by the aneurysm-specific complications, viz.: rupture penetration, embolization. The operation is performed with the aid of a temporary left-heart bypass; it carries a mortality of 5%.", "contents": "[Diagnosis and treatment of post-traumatic aneurysm of the thoracic aorta (author's transl)]. A post-traumatic false aneurysm of the thoracic aorta is generally the result of a severe closed chest trauma in which deceleration and compression mechanisms operate on the aortic isthmus. Early diagnosis is difficult except where the lesion progresses to rupture. A not inconsiderable number of incomplete and initially closed tears in the wall of the aortic isthmus escape detection and in due course lead to dilatation of the vessel and false aneurysm. The condition is diagnosed, often accidentally, during aortography. The indications for surgical intervention are given by the aneurysm-specific complications, viz.: rupture penetration, embolization. The operation is performed with the aid of a temporary left-heart bypass; it carries a mortality of 5%."}
{"id": "PMID:461335", "title": "[Post-traumatic aneurysm of the thoracic aorta; the results of surgical repair (author's transl)].", "content": "29 patients aged 11-55 years underwent surgery an aneurysm of the descending part of the thoracic aorta that had developed after a blunt chest trauma. In all patients continuity of the vessel was restored by interposition of a prosthesis. Surgery was performed during the acute stage in 5 patients, two of whom died postoperatively due to infection of the prosthesis and shock lung respectively. One of the 24 patients who were operated on during the chronic stage died on the 16th postoperative day of apoplexy. One of the 26 patients who were discharged from hospital after the operation died of a spurious aneurysm that had developed at the site of the prosthesis. Follow-up examinations of 21 patients showed normal postoperative function. In 5 cases there was an angiographically demonstrable slight narrowing at the site of the anastomosis; the pressure gradient, measured intravascularly, did not exceed 30 mm Hg.", "contents": "[Post-traumatic aneurysm of the thoracic aorta; the results of surgical repair (author's transl)]. 29 patients aged 11-55 years underwent surgery an aneurysm of the descending part of the thoracic aorta that had developed after a blunt chest trauma. In all patients continuity of the vessel was restored by interposition of a prosthesis. Surgery was performed during the acute stage in 5 patients, two of whom died postoperatively due to infection of the prosthesis and shock lung respectively. One of the 24 patients who were operated on during the chronic stage died on the 16th postoperative day of apoplexy. One of the 26 patients who were discharged from hospital after the operation died of a spurious aneurysm that had developed at the site of the prosthesis. Follow-up examinations of 21 patients showed normal postoperative function. In 5 cases there was an angiographically demonstrable slight narrowing at the site of the anastomosis; the pressure gradient, measured intravascularly, did not exceed 30 mm Hg."}
{"id": "PMID:461336", "title": "[Injury to the mediastinal organs in chest trauma (author's transl)].", "content": "10% of chest traumata are accompanied by injuries to the mediastinal organs. Since rupture of a bronchus may be masked by additional intrathoracic injuries any suspicion of such lesion must be verified by bronchoscopy. Symptoms of a shock condition after traumatization of the precordial region point to damage to the heart the extent of which may range from a slight disturbance of cardiac function (commotio cordis) to laceration of cardiac tissue and rupture of the myocardium. Enlargement of the mediastinum after a sufficiently forceful chest trauma should be examined by visualization of the aortic arch to elucidate its aetiology and exclude an aortic aneurysm. The mortality of chest trauma involving the mediastinal structures is 35%. This fairly high figure is partly attributable to severe accompanying injuries.", "contents": "[Injury to the mediastinal organs in chest trauma (author's transl)]. 10% of chest traumata are accompanied by injuries to the mediastinal organs. Since rupture of a bronchus may be masked by additional intrathoracic injuries any suspicion of such lesion must be verified by bronchoscopy. Symptoms of a shock condition after traumatization of the precordial region point to damage to the heart the extent of which may range from a slight disturbance of cardiac function (commotio cordis) to laceration of cardiac tissue and rupture of the myocardium. Enlargement of the mediastinum after a sufficiently forceful chest trauma should be examined by visualization of the aortic arch to elucidate its aetiology and exclude an aortic aneurysm. The mortality of chest trauma involving the mediastinal structures is 35%. This fairly high figure is partly attributable to severe accompanying injuries."}
{"id": "PMID:461337", "title": "[Intensive care in chest trauma (author's transl)].", "content": "A statistical analysis of the case material at the Intensive Care Unit, Freiburg, for the years 1975 and 1976 established that 40% and 39% respectively of patients with multiple injuries had also suffered a chest trauma and that the latter was the direct cause of respiratory insufficiency in 61% (1975) and 57% (1976) of patients in need of controlled respiration, i.e. respiratory insufficiency dominated the clinical and pathophysiological picture. The causes were: restricted respiratory movements due to pain, compression of the lungs or pathological changes in the injured lung, and they affected the normal gaseous exchange in a variety of ways. Alveolar hypoventilation with disturbance of ventilation-perfusion, increase in the functional shunt volume, rise in the functional dead space combined with reduced functional residual capacity and compliance result, if left uncorrected, in a drastic increase of resistance on the part of the pulmonary vessels and finally in, often fatal, hyoxaemia and hypercapnia. Regular estimations of the arterial blood gases in air and pure oxygen, of the arterio-alveolar difference in oxygen pressure, shunt volume, dead space and effective compliance of the chest wall and lungs are, therefore, essential. Treatment in an intensive care unit comprises the relief of any acute condition, such as tension pneumothorax, haemothorax, and general measures. Means to relieve pain in patients whose chest injuries are not sufficiently severe to require artificial ventilation are: intercostal blocking, acupuncture or peridural analgesia; efficient breathing exercises are important. The indications for artificial ventilation should be interpreted generously and the decision to perform it should be made at an early stage. The technique is determined by the type of pathological changes in the gaseous exchange and should aim at restoring normal conditions as far as possible.", "contents": "[Intensive care in chest trauma (author's transl)]. A statistical analysis of the case material at the Intensive Care Unit, Freiburg, for the years 1975 and 1976 established that 40% and 39% respectively of patients with multiple injuries had also suffered a chest trauma and that the latter was the direct cause of respiratory insufficiency in 61% (1975) and 57% (1976) of patients in need of controlled respiration, i.e. respiratory insufficiency dominated the clinical and pathophysiological picture. The causes were: restricted respiratory movements due to pain, compression of the lungs or pathological changes in the injured lung, and they affected the normal gaseous exchange in a variety of ways. Alveolar hypoventilation with disturbance of ventilation-perfusion, increase in the functional shunt volume, rise in the functional dead space combined with reduced functional residual capacity and compliance result, if left uncorrected, in a drastic increase of resistance on the part of the pulmonary vessels and finally in, often fatal, hyoxaemia and hypercapnia. Regular estimations of the arterial blood gases in air and pure oxygen, of the arterio-alveolar difference in oxygen pressure, shunt volume, dead space and effective compliance of the chest wall and lungs are, therefore, essential. Treatment in an intensive care unit comprises the relief of any acute condition, such as tension pneumothorax, haemothorax, and general measures. Means to relieve pain in patients whose chest injuries are not sufficiently severe to require artificial ventilation are: intercostal blocking, acupuncture or peridural analgesia; efficient breathing exercises are important. The indications for artificial ventilation should be interpreted generously and the decision to perform it should be made at an early stage. The technique is determined by the type of pathological changes in the gaseous exchange and should aim at restoring normal conditions as far as possible."}
{"id": "PMID:461338", "title": "[Surgical aspects during the intensive care of chest trauma (author's transl)].", "content": "A wide range of surgical methods is available in the intensive care of patients with a chest trauma. Discussions with the anaesthetist as to the planning and co-ordination of diagnosis and treatment must take into consideration a great number of factors. The indications for surgery in polytraumatized persons are different from those that apply to single injuries. Table 6 which lists the cause and time of death of 52 persons with multiple injuries provides evidence that during the first 7 days after a severe accident all surgical intervention that is not absolutely necessary should be avoided and attention focused on the problem of adequate ventilation and primary and secondary complications of shock.", "contents": "[Surgical aspects during the intensive care of chest trauma (author's transl)]. A wide range of surgical methods is available in the intensive care of patients with a chest trauma. Discussions with the anaesthetist as to the planning and co-ordination of diagnosis and treatment must take into consideration a great number of factors. The indications for surgery in polytraumatized persons are different from those that apply to single injuries. Table 6 which lists the cause and time of death of 52 persons with multiple injuries provides evidence that during the first 7 days after a severe accident all surgical intervention that is not absolutely necessary should be avoided and attention focused on the problem of adequate ventilation and primary and secondary complications of shock."}
{"id": "PMID:461339", "title": "[Pleural adhesions and deformation of the chest wall (author's transl)].", "content": "Deformation of the chest wall and pleural adhesions are some of the sequelae of chest trauma with fractures of ribs. If sufficiently extensive these lesions may result in restrictive disturbances of ventilation and partial respiratory insufficiency. In these cases marked improvement in function and perfusion can be obtained by decortication at an early stage. Follow-up examinations of 37 patients who had sustained fractures of more than 4 ribs revealed restrictive respiratory impairment in only three cases. Hypoxia was present in 11, but a causal connection with the trauma could be ascertained in only 4 persons. For assessing the extent of the lesions and the indications for decortication perfusion scanning is a \"must\", as it will show up pathological changes at a time when the gaseous exchange and lung function still appear normal. There seems to be no correlation between the radiological extent of the adhesions or the deformation of the thorax and the extent of changes in lung function and blood gases.", "contents": "[Pleural adhesions and deformation of the chest wall (author's transl)]. Deformation of the chest wall and pleural adhesions are some of the sequelae of chest trauma with fractures of ribs. If sufficiently extensive these lesions may result in restrictive disturbances of ventilation and partial respiratory insufficiency. In these cases marked improvement in function and perfusion can be obtained by decortication at an early stage. Follow-up examinations of 37 patients who had sustained fractures of more than 4 ribs revealed restrictive respiratory impairment in only three cases. Hypoxia was present in 11, but a causal connection with the trauma could be ascertained in only 4 persons. For assessing the extent of the lesions and the indications for decortication perfusion scanning is a \"must\", as it will show up pathological changes at a time when the gaseous exchange and lung function still appear normal. There seems to be no correlation between the radiological extent of the adhesions or the deformation of the thorax and the extent of changes in lung function and blood gases."}
{"id": "PMID:461340", "title": "[Residual lesions after serial fracture of ribs (author's transl)].", "content": "Follow-up examinations at regular intervals to recognize trauma-induced lesions are as essential for persons who have suffered a chest trauma as they are for cases of injury to the limbs. A classification into three groups according to the number of broken ribs relates satisfactorily with thoraco-pulmonary complications and residual damage. Injuries in group 1 leave practically no permanent damage; in group 2 they are apt to be followed by residual respiratory impairment while in group 3 the likelihood of permanent impairment of lung function is very high. Spirometry, roentgenography and fluoroscopy, and lung scanning are of special diagnostic value in assessing late sequelae of multiple rib fractures. Gas analyses are of little value. They would provide more accurate information if performed during exercise under standard conditions.", "contents": "[Residual lesions after serial fracture of ribs (author's transl)]. Follow-up examinations at regular intervals to recognize trauma-induced lesions are as essential for persons who have suffered a chest trauma as they are for cases of injury to the limbs. A classification into three groups according to the number of broken ribs relates satisfactorily with thoraco-pulmonary complications and residual damage. Injuries in group 1 leave practically no permanent damage; in group 2 they are apt to be followed by residual respiratory impairment while in group 3 the likelihood of permanent impairment of lung function is very high. Spirometry, roentgenography and fluoroscopy, and lung scanning are of special diagnostic value in assessing late sequelae of multiple rib fractures. Gas analyses are of little value. They would provide more accurate information if performed during exercise under standard conditions."}
{"id": "PMID:461341", "title": "[Mismanagement of chest trauma; sequels and the possibility of repair (author's transl)].", "content": "60 persons with a chest trauma were treated during the past years. In a number of cases the injuries and the resulting complications were of so serious a nature that their repair was attended by the greatest difficulties. The most serious and initially unrecognized complications comprised: 1) tracheo-bronchial defects, partly of traumatic origin and partly arising during intensive care; 2) failure to detect rupture of the diaphragm with displacement of visceral organs into the thoracic cavity; 3) insufficient evacuation of blood or fluid from the pleural cavity; 4) failure to diagnose irreparable damage to part of the lungs. Means to repair these lesions are reviewed; the necessity for long-term observation of these cases is emphasized.", "contents": "[Mismanagement of chest trauma; sequels and the possibility of repair (author's transl)]. 60 persons with a chest trauma were treated during the past years. In a number of cases the injuries and the resulting complications were of so serious a nature that their repair was attended by the greatest difficulties. The most serious and initially unrecognized complications comprised: 1) tracheo-bronchial defects, partly of traumatic origin and partly arising during intensive care; 2) failure to detect rupture of the diaphragm with displacement of visceral organs into the thoracic cavity; 3) insufficient evacuation of blood or fluid from the pleural cavity; 4) failure to diagnose irreparable damage to part of the lungs. Means to repair these lesions are reviewed; the necessity for long-term observation of these cases is emphasized."}
{"id": "PMID:461342", "title": "[Assessment of residual lesions after a chest trauma (author's transl)].", "content": "The correct assessment of residual respiratory lesions after a chest injury requires clinical examination, roentgenographic examination in two planes, lung function tests including ergo-spirometry and blood gas analyses before and after exercise. Oxygen consumption during exercise should be one of the criteria whereby to judge any reduction in working capacity. Improvements in respiratory function have been observed as late as two years after the accident.", "contents": "[Assessment of residual lesions after a chest trauma (author's transl)]. The correct assessment of residual respiratory lesions after a chest injury requires clinical examination, roentgenographic examination in two planes, lung function tests including ergo-spirometry and blood gas analyses before and after exercise. Oxygen consumption during exercise should be one of the criteria whereby to judge any reduction in working capacity. Improvements in respiratory function have been observed as late as two years after the accident."}
{"id": "PMID:461343", "title": "[Assessment of residual functional impairment after chest trauma (author's transl)].", "content": "Inadequate knowledge of the variety of residual functional lesions after a chest trauma accounts for the inconsistent assessment of these sequelae. An accurate analysis of the mode of the accident and the subsequent course greatly helps towards clarifying the situation. A final assessment should not be made until at least one year later, since improvement in respiratory function may still occur up to 12-18 months after the trauma. Tests of respiratory function should include all componente factors (ventilation, mechanics of breathing, diffusion, perfusion). An analysis of 41 cases submitted for assessment of residual respiratory lesions showed that lung scanning is the most sensitive method for detecting such damage. It revealed pathological changes in 94% of the cases, as compared with spirometric test (59%) and examination of the mechanics of breathing (33%). Whether and to what extent reduced perfusion without ergo-spirometric evidence of impairment should qualify as residual damage is a moot point.", "contents": "[Assessment of residual functional impairment after chest trauma (author's transl)]. Inadequate knowledge of the variety of residual functional lesions after a chest trauma accounts for the inconsistent assessment of these sequelae. An accurate analysis of the mode of the accident and the subsequent course greatly helps towards clarifying the situation. A final assessment should not be made until at least one year later, since improvement in respiratory function may still occur up to 12-18 months after the trauma. Tests of respiratory function should include all componente factors (ventilation, mechanics of breathing, diffusion, perfusion). An analysis of 41 cases submitted for assessment of residual respiratory lesions showed that lung scanning is the most sensitive method for detecting such damage. It revealed pathological changes in 94% of the cases, as compared with spirometric test (59%) and examination of the mechanics of breathing (33%). Whether and to what extent reduced perfusion without ergo-spirometric evidence of impairment should qualify as residual damage is a moot point."}
{"id": "PMID:461344", "title": "[Chest trauma--a challenge to the general surgeon and chest surgeon for co-operation (author's transl)].", "content": "During 1968-1977 86 persons with chest trauma were treated at the Chest Clinic Heckeshorn. Until 1973 only a few cases of, mostly slight, chest injuries were admitted and treatment was mainly concerned with pneumothorax and haemothorax induced by fracture of ribs. Since 1974, however, cases of chest trauma have markedly increased in both number and seriousness of the injury. During the past years 50 patients with chest injuries were treated. They included 6 cases of stab wounds, 4 cases of bullet wounds and one case each of damage to the trachea and bronchial rupture respectively. Thoracotomy was performed in 11 of the 50 patients, in 3 cases to stabilize the chest wall. Willingness to cooperate on the part of the general surgeon has made it possible for the surgically trained lung specialist to participate in chest traumatology.", "contents": "[Chest trauma--a challenge to the general surgeon and chest surgeon for co-operation (author's transl)]. During 1968-1977 86 persons with chest trauma were treated at the Chest Clinic Heckeshorn. Until 1973 only a few cases of, mostly slight, chest injuries were admitted and treatment was mainly concerned with pneumothorax and haemothorax induced by fracture of ribs. Since 1974, however, cases of chest trauma have markedly increased in both number and seriousness of the injury. During the past years 50 patients with chest injuries were treated. They included 6 cases of stab wounds, 4 cases of bullet wounds and one case each of damage to the trachea and bronchial rupture respectively. Thoracotomy was performed in 11 of the 50 patients, in 3 cases to stabilize the chest wall. Willingness to cooperate on the part of the general surgeon has made it possible for the surgically trained lung specialist to participate in chest traumatology."}
{"id": "PMID:461345", "title": "[A simple method for pleural drainage (author's transl)].", "content": "21 patients had pleural drainage with a rubber valve (Heimlich) on account of pneumothorax (15) and massive pleural effusion (6). The method succeeded in every case. It has the advantage that it dispenses with suction and bottles, that it needs no turning-off when the patient gets up, but maintains its draining action, and that in cases of persisting broncho-pleural fistula there is no additional suction. The technique represents a simplified alternative to the customy methods of pleural drainage.", "contents": "[A simple method for pleural drainage (author's transl)]. 21 patients had pleural drainage with a rubber valve (Heimlich) on account of pneumothorax (15) and massive pleural effusion (6). The method succeeded in every case. It has the advantage that it dispenses with suction and bottles, that it needs no turning-off when the patient gets up, but maintains its draining action, and that in cases of persisting broncho-pleural fistula there is no additional suction. The technique represents a simplified alternative to the customy methods of pleural drainage."}
{"id": "PMID:461346", "title": "[Treatment of complications of pulmonary anomalies in childhood (author's transl)].", "content": "Congenital anomalies of the lungs very often remain asymptomatic and are discovered accidentally during routine examinations. Only one-third of 24 patients with congenital pulmonary abnormalities showed acute symptoms; in a further one-third a chronic recurrent symptom complex required surgical intervention as the only possible mode of treatment. The complications arising from these anomalies varied considerably. The most serious was the \"acute thorax\" in newborns; in a number of cases it needed emergency thoracotomy. The various types and their symptoms are described and their treatment is reviewed.", "contents": "[Treatment of complications of pulmonary anomalies in childhood (author's transl)]. Congenital anomalies of the lungs very often remain asymptomatic and are discovered accidentally during routine examinations. Only one-third of 24 patients with congenital pulmonary abnormalities showed acute symptoms; in a further one-third a chronic recurrent symptom complex required surgical intervention as the only possible mode of treatment. The complications arising from these anomalies varied considerably. The most serious was the \"acute thorax\" in newborns; in a number of cases it needed emergency thoracotomy. The various types and their symptoms are described and their treatment is reviewed."}
{"id": "PMID:461347", "title": "[The tuberculosis situation in the German Federal Republic (author's transl)].", "content": "Based on a statistical anlaysis of the records the present tuberculosis situation in Germany and how it has developed during the past years are reviewed. The main means of control are: BCG vaccination, mass radiography and public health measures. Suggestions made by the Central Committee for the Control of Tuberculosis concerning surveillance and protection against occupational exposure to tuberculous infections are discussed. Recommendations regarding the assessment of incapacity for work and degree of disablement are evaluated. The aims and plans of the Committee for the next years, also in the international field, are reviewed.", "contents": "[The tuberculosis situation in the German Federal Republic (author's transl)]. Based on a statistical anlaysis of the records the present tuberculosis situation in Germany and how it has developed during the past years are reviewed. The main means of control are: BCG vaccination, mass radiography and public health measures. Suggestions made by the Central Committee for the Control of Tuberculosis concerning surveillance and protection against occupational exposure to tuberculous infections are discussed. Recommendations regarding the assessment of incapacity for work and degree of disablement are evaluated. The aims and plans of the Committee for the next years, also in the international field, are reviewed."}
{"id": "PMID:461348", "title": "[Tuberculosis in the developing countries and the work of the international Union for the control of tuberculosis (author's transl)].", "content": "In the developing countries tuberculosis is one of the most common diseases. According to estimates the number of cases of infectious tuberculosis is 3-4 millions. The guidelines for the effective control of the disease in the developing countries were laid down in a report by a Spezial Committee of the W.H.O. in 1974. The emphasis is 1) on protecting young people as far as possible by BCG vaccination, 2) on the early detection and treatment of as many cases of infectious tuberculosis as possible. The diagnosis does not depend on (expensive) radiographic equipment, as it can be made simply by microscopic examination of the sputum. For treating the infection various efficacious and not too expensive drugs are available which succeed within a few weeks in reducing the process to a non-infectious stage. Sanatorium or hospital treatment is generally not necessary.", "contents": "[Tuberculosis in the developing countries and the work of the international Union for the control of tuberculosis (author's transl)]. In the developing countries tuberculosis is one of the most common diseases. According to estimates the number of cases of infectious tuberculosis is 3-4 millions. The guidelines for the effective control of the disease in the developing countries were laid down in a report by a Spezial Committee of the W.H.O. in 1974. The emphasis is 1) on protecting young people as far as possible by BCG vaccination, 2) on the early detection and treatment of as many cases of infectious tuberculosis as possible. The diagnosis does not depend on (expensive) radiographic equipment, as it can be made simply by microscopic examination of the sputum. For treating the infection various efficacious and not too expensive drugs are available which succeed within a few weeks in reducing the process to a non-infectious stage. Sanatorium or hospital treatment is generally not necessary."}
{"id": "PMID:461349", "title": "[Death due to tuberculosis (author's transl)].", "content": "The records of autopsies performed during the last year were examined with the view of assessing the accuracy of the diagnosis: death from tuberculosis. The study showed that the infection was only rarely the cause of death. Subsequently, in collaboration with R. Gruber, the deaths reported by the Study Group, Bavaria, were analysed. Over a period of two years there were 302 deaths in which tuberculosis was either the primary or a complicating disease. The analysis shows how important it is to establish the correct diagnosis and to ensure that in deaths attributed to tuberculosis the infection is, in fact, the sole cause of death and not a complicating factor.", "contents": "[Death due to tuberculosis (author's transl)]. The records of autopsies performed during the last year were examined with the view of assessing the accuracy of the diagnosis: death from tuberculosis. The study showed that the infection was only rarely the cause of death. Subsequently, in collaboration with R. Gruber, the deaths reported by the Study Group, Bavaria, were analysed. Over a period of two years there were 302 deaths in which tuberculosis was either the primary or a complicating disease. The analysis shows how important it is to establish the correct diagnosis and to ensure that in deaths attributed to tuberculosis the infection is, in fact, the sole cause of death and not a complicating factor."}
{"id": "PMID:461350", "title": "[Severe forms of tuberculosis observed during the past year (author's transl)].", "content": "A list of all cases of tuberculosis (pulmonary tuberculosis, cavitating processes, destroyed lung) observed during 1977 was compared with a list of cases seen during the past 10 years. The incidence of cavitating tuberculosis among foreigners was 11%; men and women were involved in the ratio 68:32. The incidence of tuberculosis was related to oeconomic status and occupation. The source of infection were compared with those of 1955 and 1963. The age distribution for the severe and very severe types of tuberculosis, the stage at which the infection was first diagnosed and the incidence of relapses are reviewed. The results are tabulated.", "contents": "[Severe forms of tuberculosis observed during the past year (author's transl)]. A list of all cases of tuberculosis (pulmonary tuberculosis, cavitating processes, destroyed lung) observed during 1977 was compared with a list of cases seen during the past 10 years. The incidence of cavitating tuberculosis among foreigners was 11%; men and women were involved in the ratio 68:32. The incidence of tuberculosis was related to oeconomic status and occupation. The source of infection were compared with those of 1955 and 1963. The age distribution for the severe and very severe types of tuberculosis, the stage at which the infection was first diagnosed and the incidence of relapses are reviewed. The results are tabulated."}
{"id": "PMID:461351", "title": "[The clinical picture of acute tuberculosis (author's transl)].", "content": "The clinical picture of acute tuberculosis infection is undergoing a change. The classic symptoms of pain in the chest and night sweats are giving way to general symptoms. In 50% of the cases the infection manifests itself as an acute disease. The observation that some cases of tuberculosis are not admitted to hospital until they are in a critical condition is ascribed to lack of \"tuberculosis awareness\".", "contents": "[The clinical picture of acute tuberculosis (author's transl)]. The clinical picture of acute tuberculosis infection is undergoing a change. The classic symptoms of pain in the chest and night sweats are giving way to general symptoms. In 50% of the cases the infection manifests itself as an acute disease. The observation that some cases of tuberculosis are not admitted to hospital until they are in a critical condition is ascribed to lack of \"tuberculosis awareness\"."}
{"id": "PMID:461352", "title": "[Tuberculosis in Romania (author's transl)].", "content": "The incidence of tuberculosis in Romania has decreased considerably over the past years, particularly since 1972 when the combined rifampicin-ethambutol-pyrazinamide therapy was introduced. During the period 1968-1977 it fell from 127.9 per 100,000 to 73.4 per 100,000. The incidence of relapses fell at a slower rate. The most endangered are the 20-24 years age-group and old people; but here again the number of cases tends to fall. Childhood tuberculosis showed a yearly decline by an average of 20%. Bacteriological examinations and diagnosis are assuming increased importance.", "contents": "[Tuberculosis in Romania (author's transl)]. The incidence of tuberculosis in Romania has decreased considerably over the past years, particularly since 1972 when the combined rifampicin-ethambutol-pyrazinamide therapy was introduced. During the period 1968-1977 it fell from 127.9 per 100,000 to 73.4 per 100,000. The incidence of relapses fell at a slower rate. The most endangered are the 20-24 years age-group and old people; but here again the number of cases tends to fall. Childhood tuberculosis showed a yearly decline by an average of 20%. Bacteriological examinations and diagnosis are assuming increased importance."}
{"id": "PMID:461353", "title": "[Which type of tuberculosis predominates in cases newly diagnosed during mass radiography? (author's transl)].", "content": "12.8 millions roentgenograms, 288,000 of which showed suspicious lesions, were made during the past 25 years by the Tuberculosis Committee of Rheinland-Westfalen. They were analysed with the view of finding whether and to what extent the features of tuberculosis had changed during this period. Although severe tertiary forms of the disease had not entirely disappeared, minimal asymptomatic lesions now tended to predominate when first diagnosed--evidence of the valuable contribution made by mass radiography towards the eradication of tuberculosis. However, the picture changes somewhat if the extent of the tuberculous process is related to the number of patients in need of treatment including cases of re-activation. These figures have remained remarkably constant, viz.: the percentage of cases of unilateral tuberculosis localized to one lobe was 50-54%, of bilateral tuberculosis confined to one lobe was 22-25%; the figures for unilateral and bilateral tuberculosis involving more than one lobe were 10-13 and 10-14% respectively. The type of tuberculosis discovered during voluntary mass radiography in Rheinland-Westfalen do not yet signal an end to the proven methods of tuberculosis control.", "contents": "[Which type of tuberculosis predominates in cases newly diagnosed during mass radiography? (author's transl)]. 12.8 millions roentgenograms, 288,000 of which showed suspicious lesions, were made during the past 25 years by the Tuberculosis Committee of Rheinland-Westfalen. They were analysed with the view of finding whether and to what extent the features of tuberculosis had changed during this period. Although severe tertiary forms of the disease had not entirely disappeared, minimal asymptomatic lesions now tended to predominate when first diagnosed--evidence of the valuable contribution made by mass radiography towards the eradication of tuberculosis. However, the picture changes somewhat if the extent of the tuberculous process is related to the number of patients in need of treatment including cases of re-activation. These figures have remained remarkably constant, viz.: the percentage of cases of unilateral tuberculosis localized to one lobe was 50-54%, of bilateral tuberculosis confined to one lobe was 22-25%; the figures for unilateral and bilateral tuberculosis involving more than one lobe were 10-13 and 10-14% respectively. The type of tuberculosis discovered during voluntary mass radiography in Rheinland-Westfalen do not yet signal an end to the proven methods of tuberculosis control."}
{"id": "PMID:461354", "title": "[Liver function and anti-tuberculous drug therapy (author's transl)].", "content": "Every year since 1972 all records pertaining to an unselected group of tuberculous patients were collected on August 1st and analysed with the view of establishing trends and detecting special problems associated with anti-tuberculous chemotherapy. One of the subjects of the analysis was to find the effects of chemotherapy on the global transaminase levels. The case material was divided into two groups: in group 1 rifampicin had been included in the therapy, in group 2 it had been omitted. In approximately 20% of the patients treated during 1976, 1977 and 1978 transaminase levels were already increased when they were admitted to hospital; in about 15% transaminase levels rose during treatment. The personal history of the patients showed that in 31% of the cases the disturbed liver function was attributable to alcohol abuse and that in 22% manifest damage to the liver had been diagnosed prior to admission. Despite these \"unfavourable pre-conditions\" antituberculous drug therapy succeeded in 50% of the patients in normalizing the transaminase levels, even if the original values had been fairly high. Further controlled studies have confirmed these observations but an interpretation of the results is not yet possible. Antituberculous drug therapy induced a rise in transaminase levels less frequently than it caused a return to normal of initially raised levels. As so many factors are involved it is impossible to single out any particular causal factor.", "contents": "[Liver function and anti-tuberculous drug therapy (author's transl)]. Every year since 1972 all records pertaining to an unselected group of tuberculous patients were collected on August 1st and analysed with the view of establishing trends and detecting special problems associated with anti-tuberculous chemotherapy. One of the subjects of the analysis was to find the effects of chemotherapy on the global transaminase levels. The case material was divided into two groups: in group 1 rifampicin had been included in the therapy, in group 2 it had been omitted. In approximately 20% of the patients treated during 1976, 1977 and 1978 transaminase levels were already increased when they were admitted to hospital; in about 15% transaminase levels rose during treatment. The personal history of the patients showed that in 31% of the cases the disturbed liver function was attributable to alcohol abuse and that in 22% manifest damage to the liver had been diagnosed prior to admission. Despite these \"unfavourable pre-conditions\" antituberculous drug therapy succeeded in 50% of the patients in normalizing the transaminase levels, even if the original values had been fairly high. Further controlled studies have confirmed these observations but an interpretation of the results is not yet possible. Antituberculous drug therapy induced a rise in transaminase levels less frequently than it caused a return to normal of initially raised levels. As so many factors are involved it is impossible to single out any particular causal factor."}
{"id": "PMID:461357", "title": "[A comparative morphological and enzyme-histochemical study of experimental pneumoconiosis and experimental allergic-hyperergic pulmonary granuloma (author's transl)].", "content": "Six endotracheal applications of 15 milligrams of cadmium sulfide (CdS) given to rats at weekly intervals induce pneumoconiosis with massive adhesions. The same quantities of lead sulfide (PbS) given under the same conditions have much less effect, causing only the formation of fairly small fibrous nodules. The tissue reactions to CdS and PbS were studied morphologically (light and electron microscope) and enzyme-histochemically. The results were compared with those observed in experimental anthracosilicosis in rats and with those seen in allergic-hyperergic reactions of the lung in guinea pigs (induced by several applications of the complete Freund adjuvans). A considerable increase in macrophages in the alveoli and interstices of the lungs with increased activity of acid phosphatase and oxydoreductases had occurred in every animal. None showed primary damage to type 2 pneumocytes. The results indicate that the two toxic agents differ in their capacity to induce fibrosis not because they interfere to a different degree with the clearing function of the lung or surfactant production, but because they differ in their capacity to stimulate fibroblast formation.", "contents": "[A comparative morphological and enzyme-histochemical study of experimental pneumoconiosis and experimental allergic-hyperergic pulmonary granuloma (author's transl)]. Six endotracheal applications of 15 milligrams of cadmium sulfide (CdS) given to rats at weekly intervals induce pneumoconiosis with massive adhesions. The same quantities of lead sulfide (PbS) given under the same conditions have much less effect, causing only the formation of fairly small fibrous nodules. The tissue reactions to CdS and PbS were studied morphologically (light and electron microscope) and enzyme-histochemically. The results were compared with those observed in experimental anthracosilicosis in rats and with those seen in allergic-hyperergic reactions of the lung in guinea pigs (induced by several applications of the complete Freund adjuvans). A considerable increase in macrophages in the alveoli and interstices of the lungs with increased activity of acid phosphatase and oxydoreductases had occurred in every animal. None showed primary damage to type 2 pneumocytes. The results indicate that the two toxic agents differ in their capacity to induce fibrosis not because they interfere to a different degree with the clearing function of the lung or surfactant production, but because they differ in their capacity to stimulate fibroblast formation."}
{"id": "PMID:461358", "title": "[Roentgenologically demonstrable changes in pneumoconiosis (author's transl)].", "content": "Based on the new international definition of pneumoconiosis the various types of this lesion and their roentgenological appearance are described. The lesions may arise from fibrosis-inducing dusts (silica, asbestos) and also from the deposition of dust particles especially those of high atomic weight. Pneumoconiosis in coal miners, the most common dust-induced lung disease in Germany, is reviewed in detail and the X-ray demonstration of dust in the lungs is discussed.", "contents": "[Roentgenologically demonstrable changes in pneumoconiosis (author's transl)]. Based on the new international definition of pneumoconiosis the various types of this lesion and their roentgenological appearance are described. The lesions may arise from fibrosis-inducing dusts (silica, asbestos) and also from the deposition of dust particles especially those of high atomic weight. Pneumoconiosis in coal miners, the most common dust-induced lung disease in Germany, is reviewed in detail and the X-ray demonstration of dust in the lungs is discussed."}
{"id": "PMID:461359", "title": "[Clinical aspects and prevention of pneumoconiosis caused by inorganic dusts (author's transl)].", "content": "Anthracosilicosis and asbestosis are the most important types of pneumoconioses induced by inorganic dust. They produce entirely different damage to the broncho-pulmonary system. Anthracosilicosis graded B or C roentgenographically is very often accompanied by obstructive respiratory disorders; in asbestosis restrictive disturbances predominate. The question whether obstructive lesions also tend to occur remains to be solved. Dyspnoea often accompanied by bronchitis is the main clinical feature. Anthrocosilicosis is amenable to therapeutic measures which have resulted in a considerable prolongation of the life span of these cases. Improved dust control is the most rational prophylactic measure. Abstention from smoking also plays a part.", "contents": "[Clinical aspects and prevention of pneumoconiosis caused by inorganic dusts (author's transl)]. Anthracosilicosis and asbestosis are the most important types of pneumoconioses induced by inorganic dust. They produce entirely different damage to the broncho-pulmonary system. Anthracosilicosis graded B or C roentgenographically is very often accompanied by obstructive respiratory disorders; in asbestosis restrictive disturbances predominate. The question whether obstructive lesions also tend to occur remains to be solved. Dyspnoea often accompanied by bronchitis is the main clinical feature. Anthrocosilicosis is amenable to therapeutic measures which have resulted in a considerable prolongation of the life span of these cases. Improved dust control is the most rational prophylactic measure. Abstention from smoking also plays a part."}
{"id": "PMID:461360", "title": "[An anlysis of the post mortem material of a chest unit (author's transl)].", "content": "Tuberculous persons tend to live longer, i.e. death from tuberculosis has shifted to the older age groups. Cavitating processes frequently accompany intestinal tuberculosis, but not tuberculosis of the bones and joints. Fungus infections were found in 36 of 846 post mortem examinations, in 22 cases in association with tuberculosis.", "contents": "[An anlysis of the post mortem material of a chest unit (author's transl)]. Tuberculous persons tend to live longer, i.e. death from tuberculosis has shifted to the older age groups. Cavitating processes frequently accompany intestinal tuberculosis, but not tuberculosis of the bones and joints. Fungus infections were found in 36 of 846 post mortem examinations, in 22 cases in association with tuberculosis."}
{"id": "PMID:461361", "title": "[Early diagnosis of small airways disease in young smokers (author's transl)].", "content": "Investigations during the past years have established that determinations of closing volume, flow-volume curves and frequency-dependent compliance are capable of detecting pathological changes in the bronchial system at a time when the customary lung function tests (FEV1.0 resistance as measured by bodyplethysmography) are still normal. These observations have been made use of to diagnose incipient damage in persons exposed to bronchial noxae. In the present investigation the effect of smoking on the airways was studied in a group of 25 persons aged 20-25 years by determining the closing volume and differences in ventilatory distribution (Thews method). The results were compared with those obtained in a group of 27 non-smokers in the same age group.", "contents": "[Early diagnosis of small airways disease in young smokers (author's transl)]. Investigations during the past years have established that determinations of closing volume, flow-volume curves and frequency-dependent compliance are capable of detecting pathological changes in the bronchial system at a time when the customary lung function tests (FEV1.0 resistance as measured by bodyplethysmography) are still normal. These observations have been made use of to diagnose incipient damage in persons exposed to bronchial noxae. In the present investigation the effect of smoking on the airways was studied in a group of 25 persons aged 20-25 years by determining the closing volume and differences in ventilatory distribution (Thews method). The results were compared with those obtained in a group of 27 non-smokers in the same age group."}
{"id": "PMID:461362", "title": "[The value of lung biopsy in the radiological stage of bihilar adenopathy of sarcoidosis (author's transl)].", "content": "The classification of intrathoracic sarcoidosis into stages is based on radiological criteria. As pulmonary involvement is often considered to be an indication for corticosteroid therapy a comparison was made between the X-ray picture and lung biopsy in stage I sarcoidosis. Non-caseating epitheloid granulomas were found in 9 of 12 patients. The material was obtained by open biopsy in 8 patients and by the transbronchial approach in one case. Open and transbronchial biopsy failed in 3 patients to demonstrate sarcoid granulomas. The results suggest that the presence of mottling in the chest roentgenogram does not provide the rationale for treatment. Further studies will show whether irreversible damage to lung tissue could be prevented if lung biopsies were taken and, consequently, steroid therapy initiated, at an earlier stage of the disease.", "contents": "[The value of lung biopsy in the radiological stage of bihilar adenopathy of sarcoidosis (author's transl)]. The classification of intrathoracic sarcoidosis into stages is based on radiological criteria. As pulmonary involvement is often considered to be an indication for corticosteroid therapy a comparison was made between the X-ray picture and lung biopsy in stage I sarcoidosis. Non-caseating epitheloid granulomas were found in 9 of 12 patients. The material was obtained by open biopsy in 8 patients and by the transbronchial approach in one case. Open and transbronchial biopsy failed in 3 patients to demonstrate sarcoid granulomas. The results suggest that the presence of mottling in the chest roentgenogram does not provide the rationale for treatment. Further studies will show whether irreversible damage to lung tissue could be prevented if lung biopsies were taken and, consequently, steroid therapy initiated, at an earlier stage of the disease."}
{"id": "PMID:461363", "title": "[The value of measurements of oscillatory resistance in the evaluation of lung function. A comparative study in adults and children (author's transl)].", "content": "Measurements of total oscillatory impedance (ROS) together with spirometric and body plethysmographic determinations were made in adults and children and the results were compared. In adults (n = 260) correlation was less close (r = 0.53) than it was in 88 children (r = 0.82). Even if ROS was of similar magnitude the phase shift between pressure and flow tended to be more negative than it was in children. These differences are presumably due to anatomical differences between the adult and the growing lung. In interpretation of the results it is suggested that the differences in the ratio: airways compliance/airways resistance reflect changes arising from age and disease processes.", "contents": "[The value of measurements of oscillatory resistance in the evaluation of lung function. A comparative study in adults and children (author's transl)]. Measurements of total oscillatory impedance (ROS) together with spirometric and body plethysmographic determinations were made in adults and children and the results were compared. In adults (n = 260) correlation was less close (r = 0.53) than it was in 88 children (r = 0.82). Even if ROS was of similar magnitude the phase shift between pressure and flow tended to be more negative than it was in children. These differences are presumably due to anatomical differences between the adult and the growing lung. In interpretation of the results it is suggested that the differences in the ratio: airways compliance/airways resistance reflect changes arising from age and disease processes."}
{"id": "PMID:461364", "title": "[Propagation of error, sensitivity and specificity of the CO gas transfer in various breath holding and steady state methods (author's transl)].", "content": "For determining DL/VA or the Krogh factor use of the breath-holding CO method only is recommended because it is relatively free from errors, independent of distribution and is relatively specific regarding diffusion. The steady state methods tend to be unspecific despite calculation of DL/VA and to be rather sensitive to uneven distribution of ventilation.", "contents": "[Propagation of error, sensitivity and specificity of the CO gas transfer in various breath holding and steady state methods (author's transl)]. For determining DL/VA or the Krogh factor use of the breath-holding CO method only is recommended because it is relatively free from errors, independent of distribution and is relatively specific regarding diffusion. The steady state methods tend to be unspecific despite calculation of DL/VA and to be rather sensitive to uneven distribution of ventilation."}
{"id": "PMID:461365", "title": "[The mixing index in the argon breath holding test for the demonstration of pharmacologically induced airways reactions. A comparison with other methods (author's transl)].", "content": "In 42 patients with obstructive airways diseases the behavior of the mixing index (delta argon %/1 during phase III of the argon-volume diagram) was studied after beta2 stimulation or after provocation by acetylcholine. Changes in airways resistance resp. conductance (GAW), FEV1.0 and residual volume (RV) were also determined for comparison. In an inhomogeneous subgroup with obstructive airways disease of various aetiology the mixing index (MI) and FEV1.0 indicated changes in the airways system with about equal frequency. Distinct discrepancies between the two tests, observed in a few cases, suggest that the two methods register different pathophysiological reactions. These differences could be confirmed statistically in a more homogeneous subgroup with extrinsic bronchial asthma. A correlation could be established between MI and RV on the one hand and between FEV1.0 or GAW and RV on the other hand. The results indicate that the mixing index as a test of distribution of ventilation reflects mainly the reactions of the small airways. Its use offers the possibility of a more differentiated pharmacodynamic evaluation.", "contents": "[The mixing index in the argon breath holding test for the demonstration of pharmacologically induced airways reactions. A comparison with other methods (author's transl)]. In 42 patients with obstructive airways diseases the behavior of the mixing index (delta argon %/1 during phase III of the argon-volume diagram) was studied after beta2 stimulation or after provocation by acetylcholine. Changes in airways resistance resp. conductance (GAW), FEV1.0 and residual volume (RV) were also determined for comparison. In an inhomogeneous subgroup with obstructive airways disease of various aetiology the mixing index (MI) and FEV1.0 indicated changes in the airways system with about equal frequency. Distinct discrepancies between the two tests, observed in a few cases, suggest that the two methods register different pathophysiological reactions. These differences could be confirmed statistically in a more homogeneous subgroup with extrinsic bronchial asthma. A correlation could be established between MI and RV on the one hand and between FEV1.0 or GAW and RV on the other hand. The results indicate that the mixing index as a test of distribution of ventilation reflects mainly the reactions of the small airways. Its use offers the possibility of a more differentiated pharmacodynamic evaluation."}
{"id": "PMID:461366", "title": "[Long-term follow-up of lung function parameters in patients with idiopathic pulmonary fibrosis treated with prednisone and azathioprin or d-penicillamine (author's transl)].", "content": "The prognosis of idiopathic pulmonary fibrosis is poor, it amounts to 4-6 years (after beginning of the symptoms). Spontaneous remissions are very rare, the corticosteroid monotherapy shows a certain success in only 11-16-20% of the cases. Therefore, since 1970 we carry out a combined therapy (see above); the results, confirmed by pulmonary function tets (vital capacity, static compliance, pO2 at rest and after exercise) will be presented. In summary: (1) Azathioprin and Prednisone: After a treatment of 2 years: 6 patients improved, 2 deteriorated, 3 died; the corresponding figures after 4 years were 6/1/4, after 6 years 3/-/5, after 8 years 2/-/3; (2) D-Penicillamine and Prednisone: After 2 years 3/2/4, 1 unchanged; after 4 years 1/-/4, 1 unchanged. The results of the therapy are discussed especially regarding prognosis of the disease and used drugs.", "contents": "[Long-term follow-up of lung function parameters in patients with idiopathic pulmonary fibrosis treated with prednisone and azathioprin or d-penicillamine (author's transl)]. The prognosis of idiopathic pulmonary fibrosis is poor, it amounts to 4-6 years (after beginning of the symptoms). Spontaneous remissions are very rare, the corticosteroid monotherapy shows a certain success in only 11-16-20% of the cases. Therefore, since 1970 we carry out a combined therapy (see above); the results, confirmed by pulmonary function tets (vital capacity, static compliance, pO2 at rest and after exercise) will be presented. In summary: (1) Azathioprin and Prednisone: After a treatment of 2 years: 6 patients improved, 2 deteriorated, 3 died; the corresponding figures after 4 years were 6/1/4, after 6 years 3/-/5, after 8 years 2/-/3; (2) D-Penicillamine and Prednisone: After 2 years 3/2/4, 1 unchanged; after 4 years 1/-/4, 1 unchanged. The results of the therapy are discussed especially regarding prognosis of the disease and used drugs."}
{"id": "PMID:461367", "title": "[Bronchial asthma and auriculo-acupuncture (author's transl)].", "content": "The mode of action of acupuncture is briefly reviewed. The effect of auriculo-acupuncture is ascribed to a direct reflex action via the brain. Attempts are being made to employ auriculo-acupuncture in the treatment of acute and chronic bronchial asthma. The preliminary results are encouraging.", "contents": "[Bronchial asthma and auriculo-acupuncture (author's transl)]. The mode of action of acupuncture is briefly reviewed. The effect of auriculo-acupuncture is ascribed to a direct reflex action via the brain. Attempts are being made to employ auriculo-acupuncture in the treatment of acute and chronic bronchial asthma. The preliminary results are encouraging."}
{"id": "PMID:461368", "title": "[Acupuncture and thoraco-pulmonary function (author's transl)].", "content": "Disturbances of thoraco-pulmonary function of reflex origin can be influenced by acupuncture; it primarily affects the movements of the thorax. The most important points are enumerated. During 1958-1972 1,421 persons with bronchial asthma had acupuncture. Subjective evaluation of the results by the patients was as follows: great improvement in 45% and satisfactory improvement in 38% of the cases; 17% of the patients were not satisfied. The study continues.", "contents": "[Acupuncture and thoraco-pulmonary function (author's transl)]. Disturbances of thoraco-pulmonary function of reflex origin can be influenced by acupuncture; it primarily affects the movements of the thorax. The most important points are enumerated. During 1958-1972 1,421 persons with bronchial asthma had acupuncture. Subjective evaluation of the results by the patients was as follows: great improvement in 45% and satisfactory improvement in 38% of the cases; 17% of the patients were not satisfied. The study continues."}
{"id": "PMID:461378", "title": "[Cortisol and adipocyte indicators in exogenous-constitutional obesity].", "content": "The authors examined 64 patients with exogenous-constitutional adiposity, aged from 17 to 61 years (27 men and 37 women). The indices of the cortisol content were compared, depending on the patients' age, the degree of adiposity, heredity, and duration of the disease; the dependence and the correlation between cortisol and adipocyte morphology were ascertained. The blood cortisol content proved to depend on the patient's age and the degree of adiposity. Thus, in young patients its content was elevated; with the advance of age and increase of body weight the mean cortisol indices decreased. There was found a direct relationship between the cell size and the adiposity degree. Simultaneously marked increase of the cortisol content and marked adipocyte hypertrophy were found in individual patients. There was no increase of the cortisol content in case of normal adipocyte size. However, there was no distinct correlation between the cortisol content, the size and number of adipocytes. As the doby weight decreased the adipocyte size reduction was recorded in obese patients; some of them developed activization of the glucocorticoid function of the adrenal glands.", "contents": "[Cortisol and adipocyte indicators in exogenous-constitutional obesity]. The authors examined 64 patients with exogenous-constitutional adiposity, aged from 17 to 61 years (27 men and 37 women). The indices of the cortisol content were compared, depending on the patients' age, the degree of adiposity, heredity, and duration of the disease; the dependence and the correlation between cortisol and adipocyte morphology were ascertained. The blood cortisol content proved to depend on the patient's age and the degree of adiposity. Thus, in young patients its content was elevated; with the advance of age and increase of body weight the mean cortisol indices decreased. There was found a direct relationship between the cell size and the adiposity degree. Simultaneously marked increase of the cortisol content and marked adipocyte hypertrophy were found in individual patients. There was no increase of the cortisol content in case of normal adipocyte size. However, there was no distinct correlation between the cortisol content, the size and number of adipocytes. As the doby weight decreased the adipocyte size reduction was recorded in obese patients; some of them developed activization of the glucocorticoid function of the adrenal glands."}
{"id": "PMID:461379", "title": "[Blood lipid level in obesity associated with latent and manifest diabetes mellitus].", "content": "Free fatty acids (FFA), triglycerides, cholesterol, and blood FFA levels were studied in the glucose tolerance test (GTT) in 63 women with adiposity combined with normal and diabetic GTT, and in those suffering from manifest diabetes mellitus during reduction of body weight and normalization of carbohydrate metabolism. It appeared that normalization of body weight and of glycemia on fasting stomach and in the course of the day led to a significant normalization of the FFA, triglycerides, and cholesterol levels. Normalization of body weight alone with persisting GTT disturbances caused a significant normalization of the triglyceride and cholesterol levels; as to the FFA they remained significantly elevated. GTT normalization with persisting adiposity led to reduction of the triglycerides, cholesterol, and FFA levels, but they still significantly exceeded the parameters in healthy persons. The curve of the FFA reduction following glucose load became normal with the weight and GGT normalization, and remained delayed in cases with no loss of body weight. The data obtained permitted to draw a conclusion on the leading role of the body weight reduction in the normalization of lipid metabolism in these patients.", "contents": "[Blood lipid level in obesity associated with latent and manifest diabetes mellitus]. Free fatty acids (FFA), triglycerides, cholesterol, and blood FFA levels were studied in the glucose tolerance test (GTT) in 63 women with adiposity combined with normal and diabetic GTT, and in those suffering from manifest diabetes mellitus during reduction of body weight and normalization of carbohydrate metabolism. It appeared that normalization of body weight and of glycemia on fasting stomach and in the course of the day led to a significant normalization of the FFA, triglycerides, and cholesterol levels. Normalization of body weight alone with persisting GTT disturbances caused a significant normalization of the triglyceride and cholesterol levels; as to the FFA they remained significantly elevated. GTT normalization with persisting adiposity led to reduction of the triglycerides, cholesterol, and FFA levels, but they still significantly exceeded the parameters in healthy persons. The curve of the FFA reduction following glucose load became normal with the weight and GGT normalization, and remained delayed in cases with no loss of body weight. The data obtained permitted to draw a conclusion on the leading role of the body weight reduction in the normalization of lipid metabolism in these patients."}
{"id": "PMID:461380", "title": "[Sex hormone regulation of progesterone and estradiol receptors in the cytosol of human endometrium in normal and pathologic pregnancy].", "content": "Hormonal control of progesterone and estradiol receptors was studied in the cytozol of short-lived culture of human endometrium in normal and undeveloping pregnancy. Endometrium was cultivated in the presence of estradiol or progesterone for 16 hours. In cultivation of normal endometrium with estradiol the content of estradiol receptors increased 4-fold in comparison with unstimulated tissue, and of progesteron receptors--3-fold. In cultivation of normal endometrium with progesterone the content of estradiol receptors rose 4--5-fold, and of progesterone receptors--3-fold. In cultivation of pathological endometrium with estradiol or progesterone the number of estradiol receptors was only doubled, and of progesterone receptors--increased only 1 1/2 times, this pointing to a diminished sensitivity of pathological endometrium to the regulating action of sex hormones.", "contents": "[Sex hormone regulation of progesterone and estradiol receptors in the cytosol of human endometrium in normal and pathologic pregnancy]. Hormonal control of progesterone and estradiol receptors was studied in the cytozol of short-lived culture of human endometrium in normal and undeveloping pregnancy. Endometrium was cultivated in the presence of estradiol or progesterone for 16 hours. In cultivation of normal endometrium with estradiol the content of estradiol receptors increased 4-fold in comparison with unstimulated tissue, and of progesteron receptors--3-fold. In cultivation of normal endometrium with progesterone the content of estradiol receptors rose 4--5-fold, and of progesterone receptors--3-fold. In cultivation of pathological endometrium with estradiol or progesterone the number of estradiol receptors was only doubled, and of progesterone receptors--increased only 1 1/2 times, this pointing to a diminished sensitivity of pathological endometrium to the regulating action of sex hormones."}
{"id": "PMID:461383", "title": "[Role of serotonin receptors of the medial-basal hypothalamus in the mechanisms of negative feedback of the hypophyseal-testicular complex].", "content": "Administration of serotonin into the lateral ventricle of the brain of male rats, against the background of complete isolation of the medial-basal hypothalamus was accompanied by the block of the compensatory elevation of the blood testosterone level following unilateral castration. Experiments with local serotonin administration into the hypophysial region of the hypothalamus of hemicastrated rats indicated that the inhibitory effect produced by the excitation of the serotonin receptors of this region on the mechanism of the negative feedback of the hypophysis-testicular system was realized at the arcuate nuclei level.", "contents": "[Role of serotonin receptors of the medial-basal hypothalamus in the mechanisms of negative feedback of the hypophyseal-testicular complex]. Administration of serotonin into the lateral ventricle of the brain of male rats, against the background of complete isolation of the medial-basal hypothalamus was accompanied by the block of the compensatory elevation of the blood testosterone level following unilateral castration. Experiments with local serotonin administration into the hypophysial region of the hypothalamus of hemicastrated rats indicated that the inhibitory effect produced by the excitation of the serotonin receptors of this region on the mechanism of the negative feedback of the hypophysis-testicular system was realized at the arcuate nuclei level."}
{"id": "PMID:461385", "title": "[Interaction of insulin and its receptors in the muscles: specific I125 binding with plasma membranes of the skeletal muscle of rat].", "content": "A preparation of plasmatic membranes of the rat skeletal muscle was obtained which was used to characterize the interaction of insulin with its receptors in the myocyte. It was shown that insulin-125I binding by the membranes depended on the incubation time and temperature; the maximum binding occurred at 4 degrees, although the binding rate was low at this temperature. The degrading of labeled insulin at 4 degrees C did not exceed 1% in the course of 24 hours of incubation with the membranes. Crude pig insulin inhibited the binding by 50% in a dose of 2.7 nMol; fish insulin (biologically less active) in a dose which was 25 times greater, the other hormones studied were completely ineffective in this respect. A curve with a bend obtained by the treatment of these data after Sketchard indicated the heterogeneity of the receptors under study or their interaction of the \"negative cooperation\" type. A conclusion was drawn on the absence of any significant differences between the insulin receptors in the muscles in comparison with its receptors in other target cells.", "contents": "[Interaction of insulin and its receptors in the muscles: specific I125 binding with plasma membranes of the skeletal muscle of rat]. A preparation of plasmatic membranes of the rat skeletal muscle was obtained which was used to characterize the interaction of insulin with its receptors in the myocyte. It was shown that insulin-125I binding by the membranes depended on the incubation time and temperature; the maximum binding occurred at 4 degrees, although the binding rate was low at this temperature. The degrading of labeled insulin at 4 degrees C did not exceed 1% in the course of 24 hours of incubation with the membranes. Crude pig insulin inhibited the binding by 50% in a dose of 2.7 nMol; fish insulin (biologically less active) in a dose which was 25 times greater, the other hormones studied were completely ineffective in this respect. A curve with a bend obtained by the treatment of these data after Sketchard indicated the heterogeneity of the receptors under study or their interaction of the \"negative cooperation\" type. A conclusion was drawn on the absence of any significant differences between the insulin receptors in the muscles in comparison with its receptors in other target cells."}
{"id": "PMID:461384", "title": "[Somatotropin control of glucose transport and glycogen biosynthesis in the incubated diaphragm of hypophysectomized rats and rats with alloxan diabetes].", "content": "A study was made of the influence of somatotropic hormone of the hypophysis (STH) on the C14-glucose transport and on the C14-glucose incorporation into the glycogen of the incubated diaphragm of intact rats (group 1), hypophysectomized rats (group 2), rats with alloxan diabetes (group 3), and rats with combined exclusion of the hypophysis and the pancreas functions (group 4). The diaphragm of hypophysectomized rats had an increased capacity to the C14-glucose uptake from the medium and of incorporating it into glycogen, apparently on account of exclusion of the hormones group depending on the hypophysis, with the contrainsular action (STH, ACTH, TTH). On the contrary, the basal rate of the processes studied in the muscles of insulin-deficient rats (group 3) displayed a significant reduction. Alloxan exclusion of the B-cell function in the hypophysectomized rats (group 4) diminished the accelerated glucose transport and glycogen synthesis in the muscle. STH added to the incubation medium stimulated the glucose transport and the glycogen synthesis in the diaphragms of animals with altered endocrine homeostasis (groups 2, 3, 4). Muscles obtained from intact animals were refractory to the STH action. The most STH-sensitive were muscles of hypophysectomized animals (an increase of glucose absorption by 32%, and of C14-glucose incorporation--by 77%). Incubation of the muscles of rats suffering from alloxan diabetes with STH was also accompanied by increased rate of glucose transport and glycogen synthesis; this effect was less pronounced, however. The data obtained indicated that the regulation of carbohydrate metabolism in the muscle tissue with STH was individual, not mediated through any other hormone.", "contents": "[Somatotropin control of glucose transport and glycogen biosynthesis in the incubated diaphragm of hypophysectomized rats and rats with alloxan diabetes]. A study was made of the influence of somatotropic hormone of the hypophysis (STH) on the C14-glucose transport and on the C14-glucose incorporation into the glycogen of the incubated diaphragm of intact rats (group 1), hypophysectomized rats (group 2), rats with alloxan diabetes (group 3), and rats with combined exclusion of the hypophysis and the pancreas functions (group 4). The diaphragm of hypophysectomized rats had an increased capacity to the C14-glucose uptake from the medium and of incorporating it into glycogen, apparently on account of exclusion of the hormones group depending on the hypophysis, with the contrainsular action (STH, ACTH, TTH). On the contrary, the basal rate of the processes studied in the muscles of insulin-deficient rats (group 3) displayed a significant reduction. Alloxan exclusion of the B-cell function in the hypophysectomized rats (group 4) diminished the accelerated glucose transport and glycogen synthesis in the muscle. STH added to the incubation medium stimulated the glucose transport and the glycogen synthesis in the diaphragms of animals with altered endocrine homeostasis (groups 2, 3, 4). Muscles obtained from intact animals were refractory to the STH action. The most STH-sensitive were muscles of hypophysectomized animals (an increase of glucose absorption by 32%, and of C14-glucose incorporation--by 77%). Incubation of the muscles of rats suffering from alloxan diabetes with STH was also accompanied by increased rate of glucose transport and glycogen synthesis; this effect was less pronounced, however. The data obtained indicated that the regulation of carbohydrate metabolism in the muscle tissue with STH was individual, not mediated through any other hormone."}
{"id": "PMID:461386", "title": "[Glycogen content in the placenta of rats in experimental diabetes].", "content": "Glycogen was determined histochemically and biochemically in the placenta of female rats with alloxan diabetes induced in sexually immature animals, including the placenta of rats treated with sulfamides before the occurrence of pregnancy. Histochemical studies were carried out on 18, and biochemical on 38 Wistar rats. Glycogen content in the mature placenta before labour was greater in animals with manifest alloxan diabetes than in healthy rats. This increase of glycogen granules took place on account of their increase both in the glycogen cells, and, extracellularly, in the spongious zone. A significant increase of glycogen content in the placenta in manifest and latent diabetes was also demonstrated by biochemical methods; as to \"prediabetic\" rats--elevation of glycogen content in their placenta was insignificant in comparison with control. In diabetes compensated with sulfamides glycogen content in glycogen cells changed differently: there was a disappearance of extracellular glycogen from the spongious zone, and numerous glycogen granules (determined in healthy rats and in those with alloxan diabetes in insignificant amount) appeared in the labyrinth.", "contents": "[Glycogen content in the placenta of rats in experimental diabetes]. Glycogen was determined histochemically and biochemically in the placenta of female rats with alloxan diabetes induced in sexually immature animals, including the placenta of rats treated with sulfamides before the occurrence of pregnancy. Histochemical studies were carried out on 18, and biochemical on 38 Wistar rats. Glycogen content in the mature placenta before labour was greater in animals with manifest alloxan diabetes than in healthy rats. This increase of glycogen granules took place on account of their increase both in the glycogen cells, and, extracellularly, in the spongious zone. A significant increase of glycogen content in the placenta in manifest and latent diabetes was also demonstrated by biochemical methods; as to \"prediabetic\" rats--elevation of glycogen content in their placenta was insignificant in comparison with control. In diabetes compensated with sulfamides glycogen content in glycogen cells changed differently: there was a disappearance of extracellular glycogen from the spongious zone, and numerous glycogen granules (determined in healthy rats and in those with alloxan diabetes in insignificant amount) appeared in the labyrinth."}
{"id": "PMID:461398", "title": "[Hygienic effect of landscaping in urban areas].", "content": "The atmospheric environment in two blocks of flats with different degree of gardens and parks lain out and in a town park in Sofia was studied by the basic physical, chemical and physiologic parameters. The results give grounds to admit that laying out gardens and parks in urban zones contributes to the creation of definite, qualitatively and quantitatively different housing environment: a more favourable temperature-and-moisture, radiation - thermal and biologicaly active regime. The physiologic state of residents, looking for recreation in these places, was better in an environment of ample verdure. Laying out gardens and parks should gain wider acceptance in the urbanization of bloks of flats, since it is an effective means of improving the hygienic merits of housing environment.", "contents": "[Hygienic effect of landscaping in urban areas]. The atmospheric environment in two blocks of flats with different degree of gardens and parks lain out and in a town park in Sofia was studied by the basic physical, chemical and physiologic parameters. The results give grounds to admit that laying out gardens and parks in urban zones contributes to the creation of definite, qualitatively and quantitatively different housing environment: a more favourable temperature-and-moisture, radiation - thermal and biologicaly active regime. The physiologic state of residents, looking for recreation in these places, was better in an environment of ample verdure. Laying out gardens and parks should gain wider acceptance in the urbanization of bloks of flats, since it is an effective means of improving the hygienic merits of housing environment."}
{"id": "PMID:461399", "title": "[Age characteristics and circadian biorhythm of the leukocytes in schoolchildren].", "content": "The leucocyte counts were determined three times daily at 6 hours' interval in 480 children from 7 to 14 years of age (30 boys and 30 girls of each age group). The results showed that there exist no age or sex differences in the leucocyte counts in school-children. There was essential increase in the leucocyte counts in boys and girls from morning to evening. These data should be taken into consideration, when results of repeated determinations throughout the the day in clinical practice, sport medicine and research work are to be interpretted.", "contents": "[Age characteristics and circadian biorhythm of the leukocytes in schoolchildren]. The leucocyte counts were determined three times daily at 6 hours' interval in 480 children from 7 to 14 years of age (30 boys and 30 girls of each age group). The results showed that there exist no age or sex differences in the leucocyte counts in school-children. There was essential increase in the leucocyte counts in boys and girls from morning to evening. These data should be taken into consideration, when results of repeated determinations throughout the the day in clinical practice, sport medicine and research work are to be interpretted."}
{"id": "PMID:461388", "title": "[Effect of physical exertion on the adrenal gland function and cardiovascular system in diabetes mellitus].", "content": "The response of the adrenal glands and the cardiovascular system to dosaged cycloergometric exercises was studied in 35 young patients with diabetes, depending on the state of carbohydrate metabolism, in  comparison with control healthy individuals. In diabetic patients physical load proved to induce an insignificant activation of the adrenal glucocorticoid function with a marked response of the sympathicoadrenal system. Physical exercises permitted to detect reduction of myocardial reserves and disturbance of vascular reactivity in the majority of diabetic patients. The detected changes in the adrenal gland response could serve as one of the causes of inadequate response of the cardiovascular system to physical load, and of reduced tolerance to it in diabetic patients.", "contents": "[Effect of physical exertion on the adrenal gland function and cardiovascular system in diabetes mellitus]. The response of the adrenal glands and the cardiovascular system to dosaged cycloergometric exercises was studied in 35 young patients with diabetes, depending on the state of carbohydrate metabolism, in  comparison with control healthy individuals. In diabetic patients physical load proved to induce an insignificant activation of the adrenal glucocorticoid function with a marked response of the sympathicoadrenal system. Physical exercises permitted to detect reduction of myocardial reserves and disturbance of vascular reactivity in the majority of diabetic patients. The detected changes in the adrenal gland response could serve as one of the causes of inadequate response of the cardiovascular system to physical load, and of reduced tolerance to it in diabetic patients."}
{"id": "PMID:461400", "title": "[Degree of sexual maturation as an index of biological age].", "content": "Attempt was made to establish some characteristics of sexual maturation, depending upon chronologic and biologic age. A total of 1277 girls from 8 to 17 years of age in the city of Sofia are included in the study. There were no cases of precocious of considerably delayed pubertal development. Essential differences were found in the body dimensions, in connection with the increase in the degree of sexual maturation in one and the same chronologic age. The importance of biologic age for the individual approach to teen-agers girls and boys is emphasized.", "contents": "[Degree of sexual maturation as an index of biological age]. Attempt was made to establish some characteristics of sexual maturation, depending upon chronologic and biologic age. A total of 1277 girls from 8 to 17 years of age in the city of Sofia are included in the study. There were no cases of precocious of considerably delayed pubertal development. Essential differences were found in the body dimensions, in connection with the increase in the degree of sexual maturation in one and the same chronologic age. The importance of biologic age for the individual approach to teen-agers girls and boys is emphasized."}
{"id": "PMID:461401", "title": "[Reading and writing disorders in elementary school students with varying achievment].", "content": "In a group of 391 first and second year pupils, of whom 191 with low and 200 with excellent marks at school the author promoted studies, aimed at establishing reading and writing disorders of the so called dyslexia and dysgraphia type. Serious reading and writing disorders were detected in 14.8 per cent of all pupils in this study, disregarding their marks at school. These disorders were encountered however in 29.3 per cent of the children with low marks and in only 1 per cent of those with excellent marks. The incidence of some morbid states and functional disorders, including disorders of lateralization, was significantly higher in pupils with low marks at school. It is concluded that dyslexia and dysgraphia-type of reading and writing disorders in primary school children are factors exerting unfavourable effect on the marks at school and are likely to be etiologically related to some disturbances of health and disorders of lateralization. This prompts their early detection, with a diligent study of the causative factors and early promotion of adequate therapy and corrective measures.", "contents": "[Reading and writing disorders in elementary school students with varying achievment]. In a group of 391 first and second year pupils, of whom 191 with low and 200 with excellent marks at school the author promoted studies, aimed at establishing reading and writing disorders of the so called dyslexia and dysgraphia type. Serious reading and writing disorders were detected in 14.8 per cent of all pupils in this study, disregarding their marks at school. These disorders were encountered however in 29.3 per cent of the children with low marks and in only 1 per cent of those with excellent marks. The incidence of some morbid states and functional disorders, including disorders of lateralization, was significantly higher in pupils with low marks at school. It is concluded that dyslexia and dysgraphia-type of reading and writing disorders in primary school children are factors exerting unfavourable effect on the marks at school and are likely to be etiologically related to some disturbances of health and disorders of lateralization. This prompts their early detection, with a diligent study of the causative factors and early promotion of adequate therapy and corrective measures."}
{"id": "PMID:461402", "title": "[Effect of the protein intake on the rhythm of assimilation in white rats with proportioned feeding].", "content": "Experimental data on the influence of separate protein feeding by giving milk cassein at different hours of the day and night, apart from the energy-supplying food, to young rats, showed that protein is best utilized by the organism when given durins the dark period of the day. The results obtained are discussed in relation to the coefficients of protein and caloric effectiveness in the different hours of the day and night, with regulation of the light and dark period for 24 hours.", "contents": "[Effect of the protein intake on the rhythm of assimilation in white rats with proportioned feeding]. Experimental data on the influence of separate protein feeding by giving milk cassein at different hours of the day and night, apart from the energy-supplying food, to young rats, showed that protein is best utilized by the organism when given durins the dark period of the day. The results obtained are discussed in relation to the coefficients of protein and caloric effectiveness in the different hours of the day and night, with regulation of the light and dark period for 24 hours."}
{"id": "PMID:461403", "title": "[Mutagenic activity of basfungin in its combined action with sodium nitrite].", "content": "The mutagenic activity of basfungin, when acting in combination with sodium nitrite, was studied in a subacute experiment, using the method of bone marrow cytogenetic analysis. The results were compared with those in groups in which basfungin and sodium nitrite were applied separately, as well as with the results of the parallel control. The mutagenic effect in the combined action group did not essentially differ from the effect of application of basfungin alone, both qualitatively and diaulitatively. Interesting results were obtained in the group receiving sodium nitrite alone - the percentage of cells with chromosomal aberrations was significantly than the control values.", "contents": "[Mutagenic activity of basfungin in its combined action with sodium nitrite]. The mutagenic activity of basfungin, when acting in combination with sodium nitrite, was studied in a subacute experiment, using the method of bone marrow cytogenetic analysis. The results were compared with those in groups in which basfungin and sodium nitrite were applied separately, as well as with the results of the parallel control. The mutagenic effect in the combined action group did not essentially differ from the effect of application of basfungin alone, both qualitatively and diaulitatively. Interesting results were obtained in the group receiving sodium nitrite alone - the percentage of cells with chromosomal aberrations was significantly than the control values."}
{"id": "PMID:461404", "title": "[Potential teratogenic action of the mycelium of the higher fungus, Polyporellus squamosus].", "content": "The influence on the embryonic development of food with only source of protein derived from the mycelium of the higher fungus Polyporellus squamosus, was studied in a group of 90 pregnant rats. Animals fed caseine and standard food pressed into briquettes as source of protein served as controls. On the 17-th and 18-th gestation day 8 animals were examined and the number of of lutein bodies and fetuses was checked up. Anomalies were serched in the fetuses, by use of the method of Dawson and Wilson. The progeny of rats treated during gestation was examined on the 21-st day of life, by using a number of blood, integral and biochemical parameters (GOT, GPT, AP, SDH, catalase, sulfhydryl groups, soluble, protein). Proceeding from the results obtained, the authors rule out any teratogenic and embryotoxic activity of the mycelium of Polyporellus squamosus, under the aforegoing experimental conditions.", "contents": "[Potential teratogenic action of the mycelium of the higher fungus, Polyporellus squamosus]. The influence on the embryonic development of food with only source of protein derived from the mycelium of the higher fungus Polyporellus squamosus, was studied in a group of 90 pregnant rats. Animals fed caseine and standard food pressed into briquettes as source of protein served as controls. On the 17-th and 18-th gestation day 8 animals were examined and the number of of lutein bodies and fetuses was checked up. Anomalies were serched in the fetuses, by use of the method of Dawson and Wilson. The progeny of rats treated during gestation was examined on the 21-st day of life, by using a number of blood, integral and biochemical parameters (GOT, GPT, AP, SDH, catalase, sulfhydryl groups, soluble, protein). Proceeding from the results obtained, the authors rule out any teratogenic and embryotoxic activity of the mycelium of Polyporellus squamosus, under the aforegoing experimental conditions."}
{"id": "PMID:461405", "title": "[General toxicity and specific action of the herbicide balagrin].", "content": "The influence of the herbicide balagrin on the maternal organism and fetus was experimentally studied on 60 pregnant albino rats of Wistar line. The preparation was given orally throughout the entire gestation period in daily doses of 1/1o - 110 mg/kg, 1/20 - 55 mg/kg and 1/50 - 22 mg/kg of LD50 - 1100 mg/kg. The detoxifying function was studied by the duration of hexabarbital sleep, the excretory function - by the cholic acid content in the bile, and the enzymatic function - by the dehydrogenase activities: LDH, MDH, LDH, GL-6-PDH in the liver of pregnant animals and the fetus, Marked toxic effect on the maternal and fetal organism was demonstrable at all balagrin application levels by the liver function disturbances.", "contents": "[General toxicity and specific action of the herbicide balagrin]. The influence of the herbicide balagrin on the maternal organism and fetus was experimentally studied on 60 pregnant albino rats of Wistar line. The preparation was given orally throughout the entire gestation period in daily doses of 1/1o - 110 mg/kg, 1/20 - 55 mg/kg and 1/50 - 22 mg/kg of LD50 - 1100 mg/kg. The detoxifying function was studied by the duration of hexabarbital sleep, the excretory function - by the cholic acid content in the bile, and the enzymatic function - by the dehydrogenase activities: LDH, MDH, LDH, GL-6-PDH in the liver of pregnant animals and the fetus, Marked toxic effect on the maternal and fetal organism was demonstrable at all balagrin application levels by the liver function disturbances."}
{"id": "PMID:461406", "title": "[Morphological and histochemical changes in rabbits living for various times in the vicinity of cement factories in the villages of Beli Izvor and Zlatna Panega].", "content": "Results are reported of morphologic and histochemical studies on 60 rabbits, exposed for scattered times to the noxious effects of neighbouring cement works (gr.I - more than one year, gr.II - up to one year, and gr.III - up to 6 months - progeny of gr.I). Experiment No 1 was carried out near the village of Beli Izvor, experiment No 2 - near the village of Zlatna Panega. The results were compared with those obtained in a group of 10 normal control animals at the breeding station in Russe. The degree of morphologic changes in the organs was in direct proportion to the duration of exposure. Comparative assessment of the morphologic findings in rabbits dwelling near Beli Izvor and near Zlatna Panega showed a milder degree of organ damage in rabbits of experiment No 2 (cement works near Zlatna Penga). Inhibition of the T - and B-zones in the lymph follicles of the spleen was observed, indicating injury to the lymph (immunogenic) tissues, which are essential for the normal existence of these animals.", "contents": "[Morphological and histochemical changes in rabbits living for various times in the vicinity of cement factories in the villages of Beli Izvor and Zlatna Panega]. Results are reported of morphologic and histochemical studies on 60 rabbits, exposed for scattered times to the noxious effects of neighbouring cement works (gr.I - more than one year, gr.II - up to one year, and gr.III - up to 6 months - progeny of gr.I). Experiment No 1 was carried out near the village of Beli Izvor, experiment No 2 - near the village of Zlatna Panega. The results were compared with those obtained in a group of 10 normal control animals at the breeding station in Russe. The degree of morphologic changes in the organs was in direct proportion to the duration of exposure. Comparative assessment of the morphologic findings in rabbits dwelling near Beli Izvor and near Zlatna Panega showed a milder degree of organ damage in rabbits of experiment No 2 (cement works near Zlatna Penga). Inhibition of the T - and B-zones in the lymph follicles of the spleen was observed, indicating injury to the lymph (immunogenic) tissues, which are essential for the normal existence of these animals."}
{"id": "PMID:461407", "title": "[Occupational risk in asbestos textile manufacture].", "content": "Detailed investigations of the environment in asbestostextile industry (\"Asbestos produce\" plant in the town of Sevlievo) were carried out. The parameters determined were: overall dusting, amount of asbestos in aerosol, its mineral and disperse composition. Data on the degree of dusting in the plant for the period 1960--1976 were analyzed and summarized. Professiograms were elaborated, with chronometry and appraisal of the burden of the work operations. All workers were tested clinically and roentgenologically. The data obtained on the degree of dusting in the different departments were re-calculated as amount of inhaled asbestos, depending on how hard the work is, resp. the pulmonary ventilation. The actual risk of asbestos injury was highest in the carding department, followed by the preparatory and the spinning departments. A distinct correlation exists between the degree of professional risk, determined by the asbestos contaminations, and the clinical and laboratory findings.", "contents": "[Occupational risk in asbestos textile manufacture]. Detailed investigations of the environment in asbestostextile industry (\"Asbestos produce\" plant in the town of Sevlievo) were carried out. The parameters determined were: overall dusting, amount of asbestos in aerosol, its mineral and disperse composition. Data on the degree of dusting in the plant for the period 1960--1976 were analyzed and summarized. Professiograms were elaborated, with chronometry and appraisal of the burden of the work operations. All workers were tested clinically and roentgenologically. The data obtained on the degree of dusting in the different departments were re-calculated as amount of inhaled asbestos, depending on how hard the work is, resp. the pulmonary ventilation. The actual risk of asbestos injury was highest in the carding department, followed by the preparatory and the spinning departments. A distinct correlation exists between the degree of professional risk, determined by the asbestos contaminations, and the clinical and laboratory findings."}
{"id": "PMID:461408", "title": "[New data on the presence of submicroscopic crystalline particles in lung dusts].", "content": "Investigation of dusts, isolated from the lungs of decreased miners, has contributed much to the elucidation of the pathogenesis of dust-induced. Numerous theoretical and experimental investigations have been carried out in this field, but there are problems, which are still far from being well understood. One of these is the implication of particles less than 0,5 micron, reckoned by many authors among the air contaminators. In this report data are given on the cristal structure of particles, for which electron difraction and X-ray microanalysis have shown that they have the mineral composition of the bed, where the miner has worked.", "contents": "[New data on the presence of submicroscopic crystalline particles in lung dusts]. Investigation of dusts, isolated from the lungs of decreased miners, has contributed much to the elucidation of the pathogenesis of dust-induced. Numerous theoretical and experimental investigations have been carried out in this field, but there are problems, which are still far from being well understood. One of these is the implication of particles less than 0,5 micron, reckoned by many authors among the air contaminators. In this report data are given on the cristal structure of particles, for which electron difraction and X-ray microanalysis have shown that they have the mineral composition of the bed, where the miner has worked."}
{"id": "PMID:461409", "title": "[Atomic absorption determination of mercury vapors in the air of the work environment].", "content": "The concentration of mercury vapors in air samples obtained from work-environment may be determined by atom-adsorption technique. To this end, the most appropriate air absorption speed proved to be 0,3 dm2/min through sulphuric acid solution of potassium pemanganate. Additional oxdation of samples was performed with a mixture of sulphuric acid and nitric acid in proportion 2:1. a 24 per cent rise in the height of the adsorption peaks was thus achieved, because of reduced losses.", "contents": "[Atomic absorption determination of mercury vapors in the air of the work environment]. The concentration of mercury vapors in air samples obtained from work-environment may be determined by atom-adsorption technique. To this end, the most appropriate air absorption speed proved to be 0,3 dm2/min through sulphuric acid solution of potassium pemanganate. Additional oxdation of samples was performed with a mixture of sulphuric acid and nitric acid in proportion 2:1. a 24 per cent rise in the height of the adsorption peaks was thus achieved, because of reduced losses."}
{"id": "PMID:461410", "title": "[New sorbents for industrial gas marks with a visual reading of their depletion].", "content": "The current status of problems, referrable to the use of industrial gas masks, is reviewed, with special reference to the type of sorbent to be used, the pathways of gas mask modernization in respect of the sorbents used, and the modern trends in adsorption cartridge manufacturing; decrease of weight, alleviated construction, adjustment to serve definite toxico-chemical agent, use of ion-exchange resins and hemosorbents. Aspects are discussed for producing sorbents, permitting visual registration of filter mass exhaustion, i.e. sorbents wtih alternating color in the process of sorption, with the purpose of increasing gas mask security and efficiency. The physico-chemical parameters of reference hemosorbent samples with alternating color in the process of sorption were compared with those of widely used sorbents - active carbon (specially processed and crude) and ion-exchange resins: relative dynamics, aktivity protection time, weight, mass activity, etc. Inferences are drawn from which one may see, that these new hemosorbents are highly perspektive, and their industrial acceptance is justifiable.", "contents": "[New sorbents for industrial gas marks with a visual reading of their depletion]. The current status of problems, referrable to the use of industrial gas masks, is reviewed, with special reference to the type of sorbent to be used, the pathways of gas mask modernization in respect of the sorbents used, and the modern trends in adsorption cartridge manufacturing; decrease of weight, alleviated construction, adjustment to serve definite toxico-chemical agent, use of ion-exchange resins and hemosorbents. Aspects are discussed for producing sorbents, permitting visual registration of filter mass exhaustion, i.e. sorbents wtih alternating color in the process of sorption, with the purpose of increasing gas mask security and efficiency. The physico-chemical parameters of reference hemosorbent samples with alternating color in the process of sorption were compared with those of widely used sorbents - active carbon (specially processed and crude) and ion-exchange resins: relative dynamics, aktivity protection time, weight, mass activity, etc. Inferences are drawn from which one may see, that these new hemosorbents are highly perspektive, and their industrial acceptance is justifiable."}
{"id": "PMID:461411", "title": "[Noise characteristics of the basic types of housing construction].", "content": "The noise regime in houses, depending on the type of buildings - brick, panel and \"creeping shuttering\" type is considered. Investigations, carried out under natural conditions, showed that the type of building correlates with the noise-isolation capacity of the construction elements, in relation to the stroke noise. Poorest noise-isolation properties are to be expected from the \"creeping shuttering\" type of buildings. The characteristics of generation and spreading of noise from baths and toilets is discussed. Some tangible technical noise-protecting devices are suggested.", "contents": "[Noise characteristics of the basic types of housing construction]. The noise regime in houses, depending on the type of buildings - brick, panel and \"creeping shuttering\" type is considered. Investigations, carried out under natural conditions, showed that the type of building correlates with the noise-isolation capacity of the construction elements, in relation to the stroke noise. Poorest noise-isolation properties are to be expected from the \"creeping shuttering\" type of buildings. The characteristics of generation and spreading of noise from baths and toilets is discussed. Some tangible technical noise-protecting devices are suggested."}
{"id": "PMID:461412", "title": "[Changes in lipid metabolism in pregnant white rats and their fetuses in inhalational exposure to carbon disulfide].", "content": "It is known from the literature that carbon bisulphide affects lipid metabolism, but observations in this field are quite controversial. In the present work the authors studied the influence of carbon bisulphide on some parameters of lipid metabolism in pregnant albino rats, following inhalatory exposure to carbon bisulphide concentrations of 50, 100 and 200 mg/m3, 8 hours daily throughout the whole gestation period. The lipid metabolism changes were followed up both in two subsequent generations. The offsprings were examined at birth and after they had reached sexual maturity, at the end of gestation. Part of the animals of the basic generation were subjected to repeated carbon bisulphide treatment in the same concentrations. The following parameters were studied: total lipids, triglycerides, phospholipids, cholesterol and free fatty acids in the liver. Correlation dependencies were found between exposure carbon bisulphide concentrations and the parameters of lipid metabolism. The degree and nature of the changes depended on the concentration and type of exposure (antenatal, postnatal and combined). A distinct rise in free fatty acid levels was associated with a mild decrease in the triglyceride and phospholipid content.", "contents": "[Changes in lipid metabolism in pregnant white rats and their fetuses in inhalational exposure to carbon disulfide]. It is known from the literature that carbon bisulphide affects lipid metabolism, but observations in this field are quite controversial. In the present work the authors studied the influence of carbon bisulphide on some parameters of lipid metabolism in pregnant albino rats, following inhalatory exposure to carbon bisulphide concentrations of 50, 100 and 200 mg/m3, 8 hours daily throughout the whole gestation period. The lipid metabolism changes were followed up both in two subsequent generations. The offsprings were examined at birth and after they had reached sexual maturity, at the end of gestation. Part of the animals of the basic generation were subjected to repeated carbon bisulphide treatment in the same concentrations. The following parameters were studied: total lipids, triglycerides, phospholipids, cholesterol and free fatty acids in the liver. Correlation dependencies were found between exposure carbon bisulphide concentrations and the parameters of lipid metabolism. The degree and nature of the changes depended on the concentration and type of exposure (antenatal, postnatal and combined). A distinct rise in free fatty acid levels was associated with a mild decrease in the triglyceride and phospholipid content."}
{"id": "PMID:461432", "title": "The nutritional toxicity of Phaseolus vulgaris lectins.", "content": "In rats fed on beans (Phaseolus vulgaris) the poorly digestible lectins were shown to react with intestinal cells in vivo and to cause a disruption of many of the brush borders of duodenal and jejunal enterocytes. Although depressed to a certain extent, absorption still occurred, probably through the non-disrupted cells of the small intestine. In addition, abnormal absorption of potentially harmful substances, lectin-related or of bacterial origin, could also occur, possibly as a direct effect of the disruption caused by the lectins on the enterocytes. It is suggested that toxicity was the result of ensuing systemic effects, such as for example the observed high N excretion possibly through increased tissue catabolism.", "contents": "The nutritional toxicity of Phaseolus vulgaris lectins. In rats fed on beans (Phaseolus vulgaris) the poorly digestible lectins were shown to react with intestinal cells in vivo and to cause a disruption of many of the brush borders of duodenal and jejunal enterocytes. Although depressed to a certain extent, absorption still occurred, probably through the non-disrupted cells of the small intestine. In addition, abnormal absorption of potentially harmful substances, lectin-related or of bacterial origin, could also occur, possibly as a direct effect of the disruption caused by the lectins on the enterocytes. It is suggested that toxicity was the result of ensuing systemic effects, such as for example the observed high N excretion possibly through increased tissue catabolism."}
{"id": "PMID:461438", "title": "Malnutrition and the family: deprivation in kwashiorkor.", "content": "The background to social and emotional deprivation is discussed and applied to a study of kwashiorkor in East African children. A group of 107 children with kwashiorkor was compared with  111 controls. Age, sex and tribe were all found to have significances of their own: fifty of each group were therefore matched for these three factors. Ten other factors were found to be significant in the background of children with kwashiorkor, all of which could be associated with social or emotional deprivation or both (see Table 14). It is concluded that, in childhood, sustained personal care and affection are essential to normal growth.", "contents": "Malnutrition and the family: deprivation in kwashiorkor. The background to social and emotional deprivation is discussed and applied to a study of kwashiorkor in East African children. A group of 107 children with kwashiorkor was compared with  111 controls. Age, sex and tribe were all found to have significances of their own: fifty of each group were therefore matched for these three factors. Ten other factors were found to be significant in the background of children with kwashiorkor, all of which could be associated with social or emotional deprivation or both (see Table 14). It is concluded that, in childhood, sustained personal care and affection are essential to normal growth."}
{"id": "PMID:461476", "title": "Schedule-induced drinking and thirst: a pharmacological analysis.", "content": "Similar levels of water consumption were induced in two groups of rats by means either of prior fluid deprivation or a schedule of food pellet delivery. Injections of d-amphetamine (0.25, 0.5, 1.0 and 2.0 mg/kg) had similar attenuating effects of drinking induced by both procedures. Chlordiazepoxide (2.5, 5.0, 10 and 20 mg/kg), however, exerted differential actions on schedule-induced and deprivation-induced drinking. Drinking induced by deprivation was facilitated by all doses of this drug while the higher doses decreased levels of schedule-induced drinking. This result emphasises the difficulties involved in using the concept of thirst in explanations of behavior and of drug action.", "contents": "Schedule-induced drinking and thirst: a pharmacological analysis. Similar levels of water consumption were induced in two groups of rats by means either of prior fluid deprivation or a schedule of food pellet delivery. Injections of d-amphetamine (0.25, 0.5, 1.0 and 2.0 mg/kg) had similar attenuating effects of drinking induced by both procedures. Chlordiazepoxide (2.5, 5.0, 10 and 20 mg/kg), however, exerted differential actions on schedule-induced and deprivation-induced drinking. Drinking induced by deprivation was facilitated by all doses of this drug while the higher doses decreased levels of schedule-induced drinking. This result emphasises the difficulties involved in using the concept of thirst in explanations of behavior and of drug action."}
{"id": "PMID:461477", "title": "Exposure to a nonfunctional hot plate as a factor in the assessment of morphine-induced analgesia and analgesic tolerance in rats.", "content": "Rats not exposed to a hot plate with or without morphine and later tested on the functional hot plate with or without morphine, displayed increased paw lick latency relative to same-injected rats given pretest hot plate exposure. This analgesic effect, was termed behavioral analgesia since it, unlike morphine-induced analgesia, was not reversed by naloxone (Experiment 2). Behavioral tolerance was evident in animals exposed to the nonfunctional hot plate regardless of drug treatment and was dissociated from pharmacological tolerance. Behavioral analgesia and tolerance reported here may involve habituation to novel distractive stimuli associated with the hot plate test environment.", "contents": "Exposure to a nonfunctional hot plate as a factor in the assessment of morphine-induced analgesia and analgesic tolerance in rats. Rats not exposed to a hot plate with or without morphine and later tested on the functional hot plate with or without morphine, displayed increased paw lick latency relative to same-injected rats given pretest hot plate exposure. This analgesic effect, was termed behavioral analgesia since it, unlike morphine-induced analgesia, was not reversed by naloxone (Experiment 2). Behavioral tolerance was evident in animals exposed to the nonfunctional hot plate regardless of drug treatment and was dissociated from pharmacological tolerance. Behavioral analgesia and tolerance reported here may involve habituation to novel distractive stimuli associated with the hot plate test environment."}
{"id": "PMID:461478", "title": "Effects of cerebral intraventricular magnesium injections and a low magnesium diet on nonspecific excitability level, audiogenic seizure susceptibility and serotonin.", "content": "Cerebral intraventricular injections of 17 and 50 microgram magnesium in artificial cerebrospinal fluid progressively depressed the Non-specific Excitability Level of permanently cannulated 150--200 g rats fed a low magnesium diet for 21 days. Compared with control diet, a low Mg diet caused an increase in brain serotonin. Weanling rats fed a low Mg diet for 2 weeks, then injected with 25 microgram Mg via acute cannulations, displayed reduced audiogenic seizure susceptibility compared with sham-injected controls.", "contents": "Effects of cerebral intraventricular magnesium injections and a low magnesium diet on nonspecific excitability level, audiogenic seizure susceptibility and serotonin. Cerebral intraventricular injections of 17 and 50 microgram magnesium in artificial cerebrospinal fluid progressively depressed the Non-specific Excitability Level of permanently cannulated 150--200 g rats fed a low magnesium diet for 21 days. Compared with control diet, a low Mg diet caused an increase in brain serotonin. Weanling rats fed a low Mg diet for 2 weeks, then injected with 25 microgram Mg via acute cannulations, displayed reduced audiogenic seizure susceptibility compared with sham-injected controls."}
{"id": "PMID:461479", "title": "Evidence supporting lack of discriminative stimulus properties of a combination of naltrexone and morphine.", "content": "The aim of the present experiment was to study the potentially discriminable effects of combinations of morphine and naltrexone during long-term treatment. Three groups of gerbils had to discriminate the effects of morphine (12 mg/kg) and those of either saline (4 ml/kg), naltrexone (2 mg/kg), or a combination of this dose of morphine plus naltrexone injected IP 60 min prior to the start of the discriminative training in a T-shaped maze. Rapid development of drug discriminative control of choice behavior (left or right turn in the maze) was evident in these 3 groups which is in marked contrast to the performance of gerbils trained with morphine-naltrexone combination vs. saline or gerbils trained with naltrexone only vs. saline. Neither of these latter groups reached the criterion of performing 8 correct first-trial choices in 10 consecutive training sessions during the 60 training sessions allowed, while the 3 other groups began their criterion performance after only 7--8 training sessions. Thus the discriminative properties of certain combinations of morphine and naltrexone are weak and therfore are not easily discriminable from the effects induced by saline.", "contents": "Evidence supporting lack of discriminative stimulus properties of a combination of naltrexone and morphine. The aim of the present experiment was to study the potentially discriminable effects of combinations of morphine and naltrexone during long-term treatment. Three groups of gerbils had to discriminate the effects of morphine (12 mg/kg) and those of either saline (4 ml/kg), naltrexone (2 mg/kg), or a combination of this dose of morphine plus naltrexone injected IP 60 min prior to the start of the discriminative training in a T-shaped maze. Rapid development of drug discriminative control of choice behavior (left or right turn in the maze) was evident in these 3 groups which is in marked contrast to the performance of gerbils trained with morphine-naltrexone combination vs. saline or gerbils trained with naltrexone only vs. saline. Neither of these latter groups reached the criterion of performing 8 correct first-trial choices in 10 consecutive training sessions during the 60 training sessions allowed, while the 3 other groups began their criterion performance after only 7--8 training sessions. Thus the discriminative properties of certain combinations of morphine and naltrexone are weak and therfore are not easily discriminable from the effects induced by saline."}
{"id": "PMID:461480", "title": "Behavioral effects of aluminum ingestion on animal and human subjects.", "content": "Abnormally high brain aluminum concentrations have been detected in hemodialysis patients who died of an unexplained encephalopathy. As a result, this study was undertaken to examine whether the ingestion of aluminum produces behavioral aberrations in non-dialysed human subjects and rats with ostensibly normal renal function. Rats were fed AlCl3 by intubation in varying doses, and tests measuring learning ability, visual temporal acuity, motor coordination and activity were administered. It was found that orally ingested aluminum is absorbed by rats and deposited in the brain. High brain aluminum levels are associated with rapid general activity, decreased ability to maintain roto-rod activity, and increased sensitivity to flicker. Behavioral tests were also given to elderly human subjects and performance correlated with serum aluminum level. High serum levels of aluminum in elderly humans are associated with impaired visuo-motor coordination, poor long-term memory, and increased sensitivity to flicker.", "contents": "Behavioral effects of aluminum ingestion on animal and human subjects. Abnormally high brain aluminum concentrations have been detected in hemodialysis patients who died of an unexplained encephalopathy. As a result, this study was undertaken to examine whether the ingestion of aluminum produces behavioral aberrations in non-dialysed human subjects and rats with ostensibly normal renal function. Rats were fed AlCl3 by intubation in varying doses, and tests measuring learning ability, visual temporal acuity, motor coordination and activity were administered. It was found that orally ingested aluminum is absorbed by rats and deposited in the brain. High brain aluminum levels are associated with rapid general activity, decreased ability to maintain roto-rod activity, and increased sensitivity to flicker. Behavioral tests were also given to elderly human subjects and performance correlated with serum aluminum level. High serum levels of aluminum in elderly humans are associated with impaired visuo-motor coordination, poor long-term memory, and increased sensitivity to flicker."}
{"id": "PMID:461481", "title": "Sensorimotor deficits produced by phenytoin and chlorpromazine in unanesthetized cats.", "content": "Unanesthetized adult cats were evaluated for suprasegmental reflex activity and motor skills before and after administration of chlorpromazine (0.0625--0.5 mg/kg) alone and in combination with phenytoin (20 mg/kg). The greatest deficits were seen in the tests of balance and corrdination in which half the animals failed to match their control responses after administration of chlorpromazine and phenytoin. The impairment was most noticeable with the most stringent test (walking a 4 cm wide beam), and the effects of the two drugs were additive. Although there was no effect of either drug on muscle strength, the two drugs in combination depressed the animals' motivational state, making them less willing to work against imposed loads. Neither drug, alone or in combination, altered responses to the flexor reflex, blind placing, the hopping response or visually aided placing. It is concluded that the effects of chlorpromazine and phenytoin on motor control are selective for the CNS loci which control balance and coordination. Although the two drugs produce additive responses, the deficits occur only at doses which are well above those needed for clinical efficacy and thus may not pose a problem in their long term clinical use.", "contents": "Sensorimotor deficits produced by phenytoin and chlorpromazine in unanesthetized cats. Unanesthetized adult cats were evaluated for suprasegmental reflex activity and motor skills before and after administration of chlorpromazine (0.0625--0.5 mg/kg) alone and in combination with phenytoin (20 mg/kg). The greatest deficits were seen in the tests of balance and corrdination in which half the animals failed to match their control responses after administration of chlorpromazine and phenytoin. The impairment was most noticeable with the most stringent test (walking a 4 cm wide beam), and the effects of the two drugs were additive. Although there was no effect of either drug on muscle strength, the two drugs in combination depressed the animals' motivational state, making them less willing to work against imposed loads. Neither drug, alone or in combination, altered responses to the flexor reflex, blind placing, the hopping response or visually aided placing. It is concluded that the effects of chlorpromazine and phenytoin on motor control are selective for the CNS loci which control balance and coordination. Although the two drugs produce additive responses, the deficits occur only at doses which are well above those needed for clinical efficacy and thus may not pose a problem in their long term clinical use."}
{"id": "PMID:461482", "title": "Puromycin: two distinct behavioral effects with different temporal parameters in the pigeon.", "content": "Pigeons were injected intracerebrally with either puromycin (PM) or control saline solution following training for one 12-min session on a visual discrimination. Injections were made either immediately following training, 1 hr later or 24 hr later. Retention testing 3 days after training showed that PM produced marked amnesia in the first two groups, but had no effect in the 24 hr condition. However, all PM groups were retarded subsequently in the number of days required to reach a 90% discimination criterion. This differentiation of two separate behavioral effects with different temporal gradients suggests that PM may be working through two distinct physiological mechanisms.", "contents": "Puromycin: two distinct behavioral effects with different temporal parameters in the pigeon. Pigeons were injected intracerebrally with either puromycin (PM) or control saline solution following training for one 12-min session on a visual discrimination. Injections were made either immediately following training, 1 hr later or 24 hr later. Retention testing 3 days after training showed that PM produced marked amnesia in the first two groups, but had no effect in the 24 hr condition. However, all PM groups were retarded subsequently in the number of days required to reach a 90% discimination criterion. This differentiation of two separate behavioral effects with different temporal gradients suggests that PM may be working through two distinct physiological mechanisms."}
{"id": "PMID:461483", "title": "Imparied thermal regulation in juvenile rats following perinatal methadone exposure.", "content": "Offspring of female rats injected daily with methadone (5 mg/kg) or saline were cross-fostered at birth to form groups exposed to methadone during gestation (G), lactation (L), or gestation and lactation (G-L); controls (C) were exposed only to saline. Rectal temperature, body weight and food consumption were measured from postnatal Days 36-51. Ambient temperature was maintained at 21 degrees C except for Days 42--45, when the temperature was 10 degrees C. Group G rats never differed from controls, but offspring in Groups L and G-L were hypothermic at room temperature; Group G-L rats exhibited a further temperature loss during the cold stress. There were no group differences in food consumption after Day 39, and all groups increased food intake while in the cold. Group differences in body weight were not reliable but Group G-L rats gained less weight than the rest during the experiment, whereas Group L rats gained more. These results indicate that, depending upon treatment schedule, perinatal methadone exposure is associated with hypothermia during the postweaning period. A prolonged withdrawal reaction from methadone may account for the impaired thermal regulation.", "contents": "Imparied thermal regulation in juvenile rats following perinatal methadone exposure. Offspring of female rats injected daily with methadone (5 mg/kg) or saline were cross-fostered at birth to form groups exposed to methadone during gestation (G), lactation (L), or gestation and lactation (G-L); controls (C) were exposed only to saline. Rectal temperature, body weight and food consumption were measured from postnatal Days 36-51. Ambient temperature was maintained at 21 degrees C except for Days 42--45, when the temperature was 10 degrees C. Group G rats never differed from controls, but offspring in Groups L and G-L were hypothermic at room temperature; Group G-L rats exhibited a further temperature loss during the cold stress. There were no group differences in food consumption after Day 39, and all groups increased food intake while in the cold. Group differences in body weight were not reliable but Group G-L rats gained less weight than the rest during the experiment, whereas Group L rats gained more. These results indicate that, depending upon treatment schedule, perinatal methadone exposure is associated with hypothermia during the postweaning period. A prolonged withdrawal reaction from methadone may account for the impaired thermal regulation."}
{"id": "PMID:461484", "title": "Discriminative stimulus properties of levo-alpha-acetylmethadol and its metabolites.", "content": "The discriminative stimulus properties of l-alpha-acetylmethadol (LAAM), its metabolities 1-alpha-acetyl-N-normethadol (NorLaam) and l-alpha-acetyl-N,N-dinormethadol (DiNorLAAM), and methadone were evaluated in rats trained to discriminate between saline and morphine in a two-choice discrete-trial avoidance paradigm. All four compounds produced dose-related increases in the number of trials completed on the morphine-appropriate choice lever after either SC or oral administration indicating that the discriminative stimulus properties of the four compounds and morphine are qualitatively similar. LAAM and DiNorLAAM had a slow onset and long duration of action, and an oral: parenteral potency ratio of 1:3. NorLaam had a more rapid onset and shorter duration of action and was more potent following SC administration than either LAAM or DiNorLAAM; its oral: parenteral potency ratio was 1:10. These results are consistent with evidence from other studies that the pharmacologic activity of LAAM is dependent upon the conversion of LAAM to an active metabolite, probably NorLAAM. The similarities between DiNorLAAM and LAAM suggest that the discriminative stimulus effects of the former compound are also attributable to a metabolite.", "contents": "Discriminative stimulus properties of levo-alpha-acetylmethadol and its metabolites. The discriminative stimulus properties of l-alpha-acetylmethadol (LAAM), its metabolities 1-alpha-acetyl-N-normethadol (NorLaam) and l-alpha-acetyl-N,N-dinormethadol (DiNorLAAM), and methadone were evaluated in rats trained to discriminate between saline and morphine in a two-choice discrete-trial avoidance paradigm. All four compounds produced dose-related increases in the number of trials completed on the morphine-appropriate choice lever after either SC or oral administration indicating that the discriminative stimulus properties of the four compounds and morphine are qualitatively similar. LAAM and DiNorLAAM had a slow onset and long duration of action, and an oral: parenteral potency ratio of 1:3. NorLaam had a more rapid onset and shorter duration of action and was more potent following SC administration than either LAAM or DiNorLAAM; its oral: parenteral potency ratio was 1:10. These results are consistent with evidence from other studies that the pharmacologic activity of LAAM is dependent upon the conversion of LAAM to an active metabolite, probably NorLAAM. The similarities between DiNorLAAM and LAAM suggest that the discriminative stimulus effects of the former compound are also attributable to a metabolite."}
{"id": "PMID:461486", "title": "Facilitation by opiate antagonists of sexual performance in the male rat.", "content": "Intraperitoneal administration of increasing doses (5 mg/kg or 20 mg/kg) of the opiate antagonists naloxone hydrochloride or naltrexone hydrochloride to sexually experienced male rats caused a significant reduction in the number of intromissions prior to ejaculation and ejaculation latency. Mount and intromission latencies were significantly reduced following treatment with only the lower dose of each antagonist. Ways are suggested whereby endorphins may modulate masculine sexual performance and thereby contribute to successful fertilization of female conspecifics.", "contents": "Facilitation by opiate antagonists of sexual performance in the male rat. Intraperitoneal administration of increasing doses (5 mg/kg or 20 mg/kg) of the opiate antagonists naloxone hydrochloride or naltrexone hydrochloride to sexually experienced male rats caused a significant reduction in the number of intromissions prior to ejaculation and ejaculation latency. Mount and intromission latencies were significantly reduced following treatment with only the lower dose of each antagonist. Ways are suggested whereby endorphins may modulate masculine sexual performance and thereby contribute to successful fertilization of female conspecifics."}
{"id": "PMID:461487", "title": "Detection of antagonist activity for narcotic analgesics in mouse hot-plate test.", "content": "On the assumption that by the use of the hot-plate procedure the antagonist properties of narcotic analgesics could be detected, the effect of morphine, pentazocine, nalorphine and naloxone were investigated. The latency of paw-licking and jumping-off were determined and compared. The agonist, morphine, at doses of 0.025, 0.05 and 0.1 mmole/kg injected IP significantly increased paw-lick and jump-off latency above that seen in saline controls. The mixed agonist-antagonist, pentazocine, at doses of 0.048, 0.096 and 0.192 mmole/kg and nalorphine, an antagonist with some agonist activity, at doses of 0.032, 0.064 and 0.128 mmole/kg significantly increased the latency of paw-licking, but did not significantly change the jump-off latency. At a dose of 0.016 mmole/kg naloxone treated mice jumped from the hot-plate significantly sooner than controls but no effects of naloxone on paw-licking latency were observed. These results suggest that agonist properties are involved in the paw-lick response and that antagonistic properties determine jumping-off behavior.", "contents": "Detection of antagonist activity for narcotic analgesics in mouse hot-plate test. On the assumption that by the use of the hot-plate procedure the antagonist properties of narcotic analgesics could be detected, the effect of morphine, pentazocine, nalorphine and naloxone were investigated. The latency of paw-licking and jumping-off were determined and compared. The agonist, morphine, at doses of 0.025, 0.05 and 0.1 mmole/kg injected IP significantly increased paw-lick and jump-off latency above that seen in saline controls. The mixed agonist-antagonist, pentazocine, at doses of 0.048, 0.096 and 0.192 mmole/kg and nalorphine, an antagonist with some agonist activity, at doses of 0.032, 0.064 and 0.128 mmole/kg significantly increased the latency of paw-licking, but did not significantly change the jump-off latency. At a dose of 0.016 mmole/kg naloxone treated mice jumped from the hot-plate significantly sooner than controls but no effects of naloxone on paw-licking latency were observed. These results suggest that agonist properties are involved in the paw-lick response and that antagonistic properties determine jumping-off behavior."}
{"id": "PMID:461495", "title": "Acute effects of parathyroid extract on renal vitamin D hydroxylases in Japanese quail.", "content": "The dose- and the time-response relationships of parathyroid hormone's modulation of in vitro 1,25-(OH)2D3 and 24,25-(OH)2D3 production in Japanese quail were investigated. 4-week-old female Japanese quail were injected intramuscularly with three different doses (30, 90 and 270 USP units/kg) of parathyroid extract (PTH). 4, 12 and 24 h after the in vivo administration of PTH, kidney homogenates were incubated with tritiated 25-(OH)D3. All three doses of PTH stimulated 1,25-(OH)2D3 and inhibited 24,25-(OH)2D3 production at 12 h relative to the vehicle-treated control group. The responses of the vitamin D hydroxylases were not detectable at 4 h and had returned to control levels by 24 h. Plasma calcium rose significantly at 4 h with the two higher doese of PTH (90 and 270 USP units/mg) but not with the lowest dose. By 12 h the plasma calcium had returned to control levels except at the highest dose of PTH. By 24 h all of the plasma calcium levels had returned to control values. Plasma inorganic phosphate levels were depressed at 4 h by all three PTH doses; they remained depressed at 12 h and returned to control levels by 24 h. It is concluded that the pharmacological effects of PTH on the renal vitamin D hydroxylases are slower in onset and shorter in duration when compared with those of estradiol.", "contents": "Acute effects of parathyroid extract on renal vitamin D hydroxylases in Japanese quail. The dose- and the time-response relationships of parathyroid hormone's modulation of in vitro 1,25-(OH)2D3 and 24,25-(OH)2D3 production in Japanese quail were investigated. 4-week-old female Japanese quail were injected intramuscularly with three different doses (30, 90 and 270 USP units/kg) of parathyroid extract (PTH). 4, 12 and 24 h after the in vivo administration of PTH, kidney homogenates were incubated with tritiated 25-(OH)D3. All three doses of PTH stimulated 1,25-(OH)2D3 and inhibited 24,25-(OH)2D3 production at 12 h relative to the vehicle-treated control group. The responses of the vitamin D hydroxylases were not detectable at 4 h and had returned to control levels by 24 h. Plasma calcium rose significantly at 4 h with the two higher doese of PTH (90 and 270 USP units/mg) but not with the lowest dose. By 12 h the plasma calcium had returned to control levels except at the highest dose of PTH. By 24 h all of the plasma calcium levels had returned to control values. Plasma inorganic phosphate levels were depressed at 4 h by all three PTH doses; they remained depressed at 12 h and returned to control levels by 24 h. It is concluded that the pharmacological effects of PTH on the renal vitamin D hydroxylases are slower in onset and shorter in duration when compared with those of estradiol."}
{"id": "PMID:461496", "title": "Dose-dependent biliary and renal excretion of paracetamol in the rat.", "content": "In rats, the biliary and renal excretion of paracetamol changed in a dose-dependent manner. After an oral nonhepatotoxic dose of 200 mg/kg paractamol, the drug excreted in the bile amonted to only 5.5% within 24 h, whereas 72% of the dose were excreted into the urine. Following an oral hepatoxic dose (1,000 mg/kg), the biliary excretion of thotal paracetamol was enhanced to 13.5% whereas the renal elimination was reduced to 51% of the dose. After a nontoxic intravenous treatment with paracetamol (25-100-400 mg/kg), both the excretion of paracetamol conjugates into the bile and the elimination of free paracetamol into the urine were augmented in a dose-dependent manner. Hepatic damage due to carbon tetrachloride pretreatment (0.5 ml/kg i.p. 24 h before 100 mg/kg paracetamol i.v.) diminished both the bile flow and the biliary excretion of paracetamol conjugates, whereas the renal elimination was not affected.", "contents": "Dose-dependent biliary and renal excretion of paracetamol in the rat. In rats, the biliary and renal excretion of paracetamol changed in a dose-dependent manner. After an oral nonhepatotoxic dose of 200 mg/kg paractamol, the drug excreted in the bile amonted to only 5.5% within 24 h, whereas 72% of the dose were excreted into the urine. Following an oral hepatoxic dose (1,000 mg/kg), the biliary excretion of thotal paracetamol was enhanced to 13.5% whereas the renal elimination was reduced to 51% of the dose. After a nontoxic intravenous treatment with paracetamol (25-100-400 mg/kg), both the excretion of paracetamol conjugates into the bile and the elimination of free paracetamol into the urine were augmented in a dose-dependent manner. Hepatic damage due to carbon tetrachloride pretreatment (0.5 ml/kg i.p. 24 h before 100 mg/kg paracetamol i.v.) diminished both the bile flow and the biliary excretion of paracetamol conjugates, whereas the renal elimination was not affected."}
{"id": "PMID:461497", "title": "Effect of steroid hormones and diethylstilbestrol on adrenomedullary catecholamine secretion.", "content": "Acetylcholine-induced catecholamine secretion from isolated, perfused bovine adrenal medulla was strongly inhibited by hydrocortisone (30 micro M), estradiol (30 micro M), and estriol (30 micro M). Diethylistilbestrol (DES) (10 and 30 micro M) inhibited acetylcholine-induced secretion, and the effect of the higher dose was prolonged. Inhibition of high potassium-induced secretion by DES (30 micro M) indicated that hormonal inhibition of evoked secretion was not by competition for acetylcholine-sensitive receptor sites. Dehydrotestosterone (30 micro M) was ineffective in inhibiting acetylcholine-induced secrection. Hydrocortisone, estradiol, and estriol treatments enhanced nonstimulated release. It is suggested that incorporation of certain steroid into plasma and granule membranes, thereby modifying membrane function, enhances nonstimulated release and may also block evoked release of secretoy material.", "contents": "Effect of steroid hormones and diethylstilbestrol on adrenomedullary catecholamine secretion. Acetylcholine-induced catecholamine secretion from isolated, perfused bovine adrenal medulla was strongly inhibited by hydrocortisone (30 micro M), estradiol (30 micro M), and estriol (30 micro M). Diethylistilbestrol (DES) (10 and 30 micro M) inhibited acetylcholine-induced secretion, and the effect of the higher dose was prolonged. Inhibition of high potassium-induced secretion by DES (30 micro M) indicated that hormonal inhibition of evoked secretion was not by competition for acetylcholine-sensitive receptor sites. Dehydrotestosterone (30 micro M) was ineffective in inhibiting acetylcholine-induced secrection. Hydrocortisone, estradiol, and estriol treatments enhanced nonstimulated release. It is suggested that incorporation of certain steroid into plasma and granule membranes, thereby modifying membrane function, enhances nonstimulated release and may also block evoked release of secretoy material."}
{"id": "PMID:461498", "title": "Assay of antipyrine and its primary metabolites in plasma, saliva and urine by high-performance liquid chromatography and some preliminary results in man.", "content": "A reversed phase system for the HPLC separation of antipyrine and its primary metabolites is described. Based on this system an assay procedure for antipyrine in plasma and saliva was developed with a lowest measurable concentration of 25 ng/ml and precision of +/- 3.6 and +/- 4.5%, respectively. Furthermore, assays for the parent compound, 3-hydroxymethyl-antipyrine, norantipyrine and 4-hydroxy-antipyrine in urine were developed. The lowest measurable concentration for these compounds is about 100 ng/ml except for 3-hydroxymethyl-antipyrine with a lowest measurable concentration of about 200 ng/ml. The precision was established at +/- 3.6 and +/- 5.0% for 3-hydroxymethyl-antipyrine, and antipyrine, respectively, and +/- 7.0 and +/- 3.6% for norantipyrine and 4-hydroxy-antipyrine, respectively. The method was applied to studies on antipyrine metabolism in humans. Following administration of a single dose of 500 mg antipyrine to 5 healthy volunteers, 3.3 +/- 1.2% of the dose was recovered from 48-hour hydrolyzed urine as unchanged drug, 39.7+/- 8.7% as 3-hydroxymethyl-antipyrine, 14.5 +/- 6.8% as norantipyrine and 28.5 +/- 2.2% as 4-hydroxy-antipyrine.", "contents": "Assay of antipyrine and its primary metabolites in plasma, saliva and urine by high-performance liquid chromatography and some preliminary results in man. A reversed phase system for the HPLC separation of antipyrine and its primary metabolites is described. Based on this system an assay procedure for antipyrine in plasma and saliva was developed with a lowest measurable concentration of 25 ng/ml and precision of +/- 3.6 and +/- 4.5%, respectively. Furthermore, assays for the parent compound, 3-hydroxymethyl-antipyrine, norantipyrine and 4-hydroxy-antipyrine in urine were developed. The lowest measurable concentration for these compounds is about 100 ng/ml except for 3-hydroxymethyl-antipyrine with a lowest measurable concentration of about 200 ng/ml. The precision was established at +/- 3.6 and +/- 5.0% for 3-hydroxymethyl-antipyrine, and antipyrine, respectively, and +/- 7.0 and +/- 3.6% for norantipyrine and 4-hydroxy-antipyrine, respectively. The method was applied to studies on antipyrine metabolism in humans. Following administration of a single dose of 500 mg antipyrine to 5 healthy volunteers, 3.3 +/- 1.2% of the dose was recovered from 48-hour hydrolyzed urine as unchanged drug, 39.7+/- 8.7% as 3-hydroxymethyl-antipyrine, 14.5 +/- 6.8% as norantipyrine and 28.5 +/- 2.2% as 4-hydroxy-antipyrine."}
{"id": "PMID:461500", "title": "Pharmaco-electroencephalography: the value of quantified EEG in psychopharmacology.", "content": "One of the first routine applications of quantitative EEG analysis was in the field of human psychopharmacology. As early as the 1960's, quantitative techniques, mainly period and amplitude integration analyses or analogue filtering, were successfully applied to EEG data with the aim of quantifying and objectifying changes induced by psychoactive medidation. Thus, step by step, a new research field, pharmacoelectroencephalography, was developed. Many other sophisticated new techniques have been described in great detail from the engineering viewpoint. However, due to the language barriers common between representatives of the various disciplines involved, it is still difficult to view them in context with the experimental design as a whole. Therefore, based on examples taken from our routine work, an attempt is made to give a balanced judgement on the value of such techniques in pharmaco-electroencephalography, and their contribution to the CNS pharmacology.", "contents": "Pharmaco-electroencephalography: the value of quantified EEG in psychopharmacology. One of the first routine applications of quantitative EEG analysis was in the field of human psychopharmacology. As early as the 1960's, quantitative techniques, mainly period and amplitude integration analyses or analogue filtering, were successfully applied to EEG data with the aim of quantifying and objectifying changes induced by psychoactive medidation. Thus, step by step, a new research field, pharmacoelectroencephalography, was developed. Many other sophisticated new techniques have been described in great detail from the engineering viewpoint. However, due to the language barriers common between representatives of the various disciplines involved, it is still difficult to view them in context with the experimental design as a whole. Therefore, based on examples taken from our routine work, an attempt is made to give a balanced judgement on the value of such techniques in pharmaco-electroencephalography, and their contribution to the CNS pharmacology."}
{"id": "PMID:461501", "title": "Vigilance and evaluation of psychotropic drug effects on EEG.", "content": "Dynamics and structure of EEG-patterning are highly sensitive indicators of psychotropic drug effects on the regulation of vigilance. Methodological requirements for an adequate evaluation of these effects and appropriate procedures for their measurement are discussed. The kind of information to be obtained by using these strategies is illustrated by the results of comparative investigations concerning the action of Viloxazine, a new antidepressant drug, on the resting activity and on the waking EEG during performance of various tracking tasks.", "contents": "Vigilance and evaluation of psychotropic drug effects on EEG. Dynamics and structure of EEG-patterning are highly sensitive indicators of psychotropic drug effects on the regulation of vigilance. Methodological requirements for an adequate evaluation of these effects and appropriate procedures for their measurement are discussed. The kind of information to be obtained by using these strategies is illustrated by the results of comparative investigations concerning the action of Viloxazine, a new antidepressant drug, on the resting activity and on the waking EEG during performance of various tracking tasks."}
{"id": "PMID:461502", "title": "Automatic measurement of the vigilance level and its possible application in psychopharmacology.", "content": "The main object of the study was to separate the specific EEG effects of psychopharmaca from changes caused by drowsiness. The study was based on quantitative evaluation of the vigilance level during five minutes of EEG recording under standardized conditions. The equation for computing the vigilance level from EEG spectra was obtained by using a training material. The segments were classified visually either as alert EEG, or as EEG marked by drowsiness. As confirmed by independent test material, the coefficients derived by means of multiple linear regression were applicable to other EEGs for estimating the vigilance level. The procedure was used in the evaluation of the psychopharmacological treatment. It was concluded that the specific EEG drug profiles were certainly influenced by the accompanying drowsiness, but the main information was still preserved. In contrast to the specific drug effect, the drug-induced sleep activity could be isolated from other events and studied separately. It was also found that after a standard stimulation, both the general trend and periodicity of the vigilance fluctuations were closely related to the psychic state of the patient.", "contents": "Automatic measurement of the vigilance level and its possible application in psychopharmacology. The main object of the study was to separate the specific EEG effects of psychopharmaca from changes caused by drowsiness. The study was based on quantitative evaluation of the vigilance level during five minutes of EEG recording under standardized conditions. The equation for computing the vigilance level from EEG spectra was obtained by using a training material. The segments were classified visually either as alert EEG, or as EEG marked by drowsiness. As confirmed by independent test material, the coefficients derived by means of multiple linear regression were applicable to other EEGs for estimating the vigilance level. The procedure was used in the evaluation of the psychopharmacological treatment. It was concluded that the specific EEG drug profiles were certainly influenced by the accompanying drowsiness, but the main information was still preserved. In contrast to the specific drug effect, the drug-induced sleep activity could be isolated from other events and studied separately. It was also found that after a standard stimulation, both the general trend and periodicity of the vigilance fluctuations were closely related to the psychic state of the patient."}
{"id": "PMID:461503", "title": "The significance of personality traits in EEG evaluation of drug effects.", "content": "The relatively clear and compact picture of an electroencephalographic classification of psychotropic substances becomes extremely more complex if the influence of personality traits and topographic differentiations in the EEG are taken into account. Using the example of EEG alterations under diazepam, amitriptyline, pyritinol, EMD 21-657 and caffeine, the complex structure of substance effects, personality factors, topographical differentiations, placebo reactivity and spontaneous circadian variability of the EEG is demonstrated.", "contents": "The significance of personality traits in EEG evaluation of drug effects. The relatively clear and compact picture of an electroencephalographic classification of psychotropic substances becomes extremely more complex if the influence of personality traits and topographic differentiations in the EEG are taken into account. Using the example of EEG alterations under diazepam, amitriptyline, pyritinol, EMD 21-657 and caffeine, the complex structure of substance effects, personality factors, topographical differentiations, placebo reactivity and spontaneous circadian variability of the EEG is demonstrated."}
{"id": "PMID:461507", "title": "Statistical handling of EEG-data.", "content": "This contribution focusses on the treatment of EEG data as time series. More emphasis is placed on spontaneous activity than on event-related potentials. Smoothing techniques are discussed, both for the nonparametric estimation of regression functions and of densities. Some new promising methods are presented. The fundamentals of time series analysis, and in particular of spectrum analysis, are reviewed. For the difficult area of parameterization of EEG time series, we compare desirable characteristica of parameters with the methods already in use.", "contents": "Statistical handling of EEG-data. This contribution focusses on the treatment of EEG data as time series. More emphasis is placed on spontaneous activity than on event-related potentials. Smoothing techniques are discussed, both for the nonparametric estimation of regression functions and of densities. Some new promising methods are presented. The fundamentals of time series analysis, and in particular of spectrum analysis, are reviewed. For the difficult area of parameterization of EEG time series, we compare desirable characteristica of parameters with the methods already in use."}
{"id": "PMID:461509", "title": "Statistical problems in the analysis of comparative pharmaco-EEG trials.", "content": "Assuming already condensed data from the EEG power spectra, problems arising with the statistical analysis of such data in comparative pharmaco-EEG trials are discussed. Since, in general, there is only a limited number of subjects available in a study to evaluate and compare the effects of a number of drugs by means of the EEG, the experimental design is usually of the cross-over type. With respect to this design, the requirements for the application and for the appropriate analysis are stated. Further, the problems are indicated which arise when the number of subjects is not a multiple of the number of drugs and/or when there are missing values. Finally, the topic of repeated null hypothesis testing and the inflation of the overall significance level is treated. Suggestions are made how to at least partly avoid the indicated problems at the present stage of methodological statistical research.", "contents": "Statistical problems in the analysis of comparative pharmaco-EEG trials. Assuming already condensed data from the EEG power spectra, problems arising with the statistical analysis of such data in comparative pharmaco-EEG trials are discussed. Since, in general, there is only a limited number of subjects available in a study to evaluate and compare the effects of a number of drugs by means of the EEG, the experimental design is usually of the cross-over type. With respect to this design, the requirements for the application and for the appropriate analysis are stated. Further, the problems are indicated which arise when the number of subjects is not a multiple of the number of drugs and/or when there are missing values. Finally, the topic of repeated null hypothesis testing and the inflation of the overall significance level is treated. Suggestions are made how to at least partly avoid the indicated problems at the present stage of methodological statistical research."}
{"id": "PMID:461510", "title": "Computation of bremsstrahlung X-ray spectra and comparison with spectra measured with a Ge(Li) detector.", "content": "A method of computing theoretical X-ray spectra in the range 30-150 kV is presented. The theoretical spectra are compared with constant potential, high resolution spectra from a tungsten target measured with a Ge(Li) detector, for a range of target angles, tube voltage and filtrations. Above 100 kV the spectra were also measured with a NaI detector but, as there was good agreement between the Ge(Li) and NaI detectors, only the former are presented. Spectra computed using Kramers' theory are also included for comparison, giving fairly good agreement at large target angles (30 degrees) but becoming gradually worse as the target angle decreased. Spectra may be computed by this method for any desired filtration, target angle, and tube voltage between 30 and 150 kV, in excellent agreement with the measured data.", "contents": "Computation of bremsstrahlung X-ray spectra and comparison with spectra measured with a Ge(Li) detector. A method of computing theoretical X-ray spectra in the range 30-150 kV is presented. The theoretical spectra are compared with constant potential, high resolution spectra from a tungsten target measured with a Ge(Li) detector, for a range of target angles, tube voltage and filtrations. Above 100 kV the spectra were also measured with a NaI detector but, as there was good agreement between the Ge(Li) and NaI detectors, only the former are presented. Spectra computed using Kramers' theory are also included for comparison, giving fairly good agreement at large target angles (30 degrees) but becoming gradually worse as the target angle decreased. Spectra may be computed by this method for any desired filtration, target angle, and tube voltage between 30 and 150 kV, in excellent agreement with the measured data."}
{"id": "PMID:461511", "title": "Determination of the stopping cross-sections of N2, H2, CH4, C4H10 and C3H6 using alpha particles in the range 1 . 3--4 . 2 MeV.", "content": "The stopping cross-sections of N2, H2, CH4, C4H10 and C3H6 have been measured using natural alpha particles from a 238Pu source, with facilities to vary the energy in the range 1.3--4.2 MeV. The work is the extension of measurements made earlier, using C2H4 as the absorber, which showed discrepancies in comparison with results of other authors. The comparison is continued with the results presented here and brief comments made on the degree of agreement obtained.", "contents": "Determination of the stopping cross-sections of N2, H2, CH4, C4H10 and C3H6 using alpha particles in the range 1 . 3--4 . 2 MeV. The stopping cross-sections of N2, H2, CH4, C4H10 and C3H6 have been measured using natural alpha particles from a 238Pu source, with facilities to vary the energy in the range 1.3--4.2 MeV. The work is the extension of measurements made earlier, using C2H4 as the absorber, which showed discrepancies in comparison with results of other authors. The comparison is continued with the results presented here and brief comments made on the degree of agreement obtained."}
{"id": "PMID:461512", "title": "The calculation of annual limits of intake for plutonium-239 in man using a bone model which allows for plutonium burial and recycling.", "content": "Values of the annual limit of intake (ALI) for plutonium-239 in man have been calculated using committed dose equivalent limits as recommended by ICRP in Publication 26. The calculations were made using a multicompartment bone model which allows for plutonium burial and recycling in the skeleton. In one skeletal compartment, the growing surfaces of cortical bone, it is assumed that plutonium deposits are retained and are not subject to resorption or recycling. In the trabecular bone compartment plutonium is taken to be resorbed with either subsequent redeposition onto bone surfaces or retention in the bone marrow. ALIs for plutonium-239 have been calculated assuming a range of rates of bone accretion (0-32 micron yr-1), different amounts of plutonium retained in the marrow (0-60%) and a 20%, 45% or 70% deposition of plutonium in the skeleton from the blood. The calculations made using this bone model suggest that 750 Bq (20 nCi) is an appropriate ALI for the inhalation of class W and class Y plutonium compounds and that 830 kBq and 5 MBq (23 muCi and 136 muCi) are the appropriate ALIs for the ingestion of soluble and insoluble forms of plutonium respectively.", "contents": "The calculation of annual limits of intake for plutonium-239 in man using a bone model which allows for plutonium burial and recycling. Values of the annual limit of intake (ALI) for plutonium-239 in man have been calculated using committed dose equivalent limits as recommended by ICRP in Publication 26. The calculations were made using a multicompartment bone model which allows for plutonium burial and recycling in the skeleton. In one skeletal compartment, the growing surfaces of cortical bone, it is assumed that plutonium deposits are retained and are not subject to resorption or recycling. In the trabecular bone compartment plutonium is taken to be resorbed with either subsequent redeposition onto bone surfaces or retention in the bone marrow. ALIs for plutonium-239 have been calculated assuming a range of rates of bone accretion (0-32 micron yr-1), different amounts of plutonium retained in the marrow (0-60%) and a 20%, 45% or 70% deposition of plutonium in the skeleton from the blood. The calculations made using this bone model suggest that 750 Bq (20 nCi) is an appropriate ALI for the inhalation of class W and class Y plutonium compounds and that 830 kBq and 5 MBq (23 muCi and 136 muCi) are the appropriate ALIs for the ingestion of soluble and insoluble forms of plutonium respectively."}
{"id": "PMID:461515", "title": "Contributions to depth discrimination gamma-gamma-coincidence methods in scintigraphy.", "content": "Two problems in the gamma-gamma-coincidence method for depth discrimination in scintigraphy are discussed. Firstly, the FW (1/e)M of the distribution function of time differences between 'coincident' pulses is calculated to be the optimum resolving time for measurement of true coincidences. Secondly, the improvement of time resolution of such an apparatus by substituting a large volume NaI(TI) crystal by an array of many small ones is analysed. It is shown that such arrays will have no practical advantage since the loss in efficiency is of the same order of magnitude as the gain in time resolution.", "contents": "Contributions to depth discrimination gamma-gamma-coincidence methods in scintigraphy. Two problems in the gamma-gamma-coincidence method for depth discrimination in scintigraphy are discussed. Firstly, the FW (1/e)M of the distribution function of time differences between 'coincident' pulses is calculated to be the optimum resolving time for measurement of true coincidences. Secondly, the improvement of time resolution of such an apparatus by substituting a large volume NaI(TI) crystal by an array of many small ones is analysed. It is shown that such arrays will have no practical advantage since the loss in efficiency is of the same order of magnitude as the gain in time resolution."}
{"id": "PMID:461516", "title": "A study of the application of a deconvolution method to scintigraphy.", "content": "It is shown that an Anger-type gamma camera can be considered as a linear filter. The image is therefore the convolution of the object by the camera point spread function. An important property of the objects is the fact that they are basically positive (count-rates as a function of space variables). The proposed deconvolution method (due to Biraud) is shown to work satisfactorily on a 1-D scintigraphic signal which is a particular cross-section of a 2-D image. This is a preliminary study of the enhancement of real scintigraphic images.", "contents": "A study of the application of a deconvolution method to scintigraphy. It is shown that an Anger-type gamma camera can be considered as a linear filter. The image is therefore the convolution of the object by the camera point spread function. An important property of the objects is the fact that they are basically positive (count-rates as a function of space variables). The proposed deconvolution method (due to Biraud) is shown to work satisfactorily on a 1-D scintigraphic signal which is a particular cross-section of a 2-D image. This is a preliminary study of the enhancement of real scintigraphic images."}
{"id": "PMID:461517", "title": "Thermal recovery of the skin after cooling.", "content": "A theoretical model of the skin is used to study the thermal recovery of the skin temperature after cooling. The analytical solution of the partial differential equation describing this model shows that recovery curves can be approximated by a two-exponential curve or even by a mono-exponential curve when the data from the first two minutes are omitted. With this model, it is possible to study the influence of the blood flow and the thermal conductivity lambda of the skin on the steady-state temperature and the decay constants mun. New experiments will be carried out to verify the relation between mu1, mu2  and the blood flow but now correcting for the influence of lambda, as predicted by the model.", "contents": "Thermal recovery of the skin after cooling. A theoretical model of the skin is used to study the thermal recovery of the skin temperature after cooling. The analytical solution of the partial differential equation describing this model shows that recovery curves can be approximated by a two-exponential curve or even by a mono-exponential curve when the data from the first two minutes are omitted. With this model, it is possible to study the influence of the blood flow and the thermal conductivity lambda of the skin on the steady-state temperature and the decay constants mun. New experiments will be carried out to verify the relation between mu1, mu2  and the blood flow but now correcting for the influence of lambda, as predicted by the model."}
{"id": "PMID:461519", "title": "A study of ultrasonically induced pulsations of gas-filled channels in Elodea.", "content": "The ultrasonically stimulated pulsation of pockets of in elodea has been studied using optical microscopy. The intensity dependence of the microstreaming associated with these pulsations has been measured by observing the rotation of chloroplasts and other organelles in the microstreaming vortices. The threshold energy density to produce microstreaming in a standing wave field was found to be 0 . 03 J m-3 at 700 kHz, equivalent to an average intensity of 5 mW cm-2 in the standing wave field, and 0 . 01 J m-3 at 3 MHz, equivalent to an intensity of 15 mW cm-2. The channels had resonant frequencies in the low megahertz range and the relationship between the channel diameter and the resonant frequency was found to be similar to that for a gas bubble in a liquid.", "contents": "A study of ultrasonically induced pulsations of gas-filled channels in Elodea. The ultrasonically stimulated pulsation of pockets of in elodea has been studied using optical microscopy. The intensity dependence of the microstreaming associated with these pulsations has been measured by observing the rotation of chloroplasts and other organelles in the microstreaming vortices. The threshold energy density to produce microstreaming in a standing wave field was found to be 0 . 03 J m-3 at 700 kHz, equivalent to an average intensity of 5 mW cm-2 in the standing wave field, and 0 . 01 J m-3 at 3 MHz, equivalent to an intensity of 15 mW cm-2. The channels had resonant frequencies in the low megahertz range and the relationship between the channel diameter and the resonant frequency was found to be similar to that for a gas bubble in a liquid."}
{"id": "PMID:461520", "title": "Influence of beam characteristics and detector size in negative-pion dose studies.", "content": "Dose calculations were performed for a tissue phantom irradiated by uniform circular beams of negative pions with an assumed gaussian momentum distribution. The mean momentum of the pions was varied from 104.4 to 171.5 MeV/c (mean range 5-20 cm in unit-density tissue) and the momentum spread from 0 to 5% of the mean. Depth-dose curves are shown for different mean momenta and momentum spreads. The radial distribution of dose as a function of distance from the beam axis was computed at different depths for a beam with a mean momentum of 153.4 MeV/c and spread of 2%. The responses of detectors of different sizes used to measure centre-line dose for this beam were shown by calculating depth-dose curves for detectors of radii 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 cm. Calculations were also performed for beams having radii of 1, 2 and 3 cm. Depending on particular conditions, it appears that considerable care may often be needed to infer the absorbed dose at a given location in a phantom irradiated by a negative-pion beam.", "contents": "Influence of beam characteristics and detector size in negative-pion dose studies. Dose calculations were performed for a tissue phantom irradiated by uniform circular beams of negative pions with an assumed gaussian momentum distribution. The mean momentum of the pions was varied from 104.4 to 171.5 MeV/c (mean range 5-20 cm in unit-density tissue) and the momentum spread from 0 to 5% of the mean. Depth-dose curves are shown for different mean momenta and momentum spreads. The radial distribution of dose as a function of distance from the beam axis was computed at different depths for a beam with a mean momentum of 153.4 MeV/c and spread of 2%. The responses of detectors of different sizes used to measure centre-line dose for this beam were shown by calculating depth-dose curves for detectors of radii 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 cm. Calculations were also performed for beams having radii of 1, 2 and 3 cm. Depending on particular conditions, it appears that considerable care may often be needed to infer the absorbed dose at a given location in a phantom irradiated by a negative-pion beam."}
{"id": "PMID:461521", "title": "Theoretical determination of scatter factors at depths in walls of cylindrical chambers.", "content": "A theoretical evaluation has been made of the contribution from scattered photons to dose at depths in the walls of cylindrical cavity chambers for photon beams in the energy range 2-30 MV. Scatter factors calculated for graphite, Perspex and water walls over a range of depths and thicknesses show satisfactory agreement with the experimental results of other workers.", "contents": "Theoretical determination of scatter factors at depths in walls of cylindrical chambers. A theoretical evaluation has been made of the contribution from scattered photons to dose at depths in the walls of cylindrical cavity chambers for photon beams in the energy range 2-30 MV. Scatter factors calculated for graphite, Perspex and water walls over a range of depths and thicknesses show satisfactory agreement with the experimental results of other workers."}
{"id": "PMID:461522", "title": "Air-gap correction in electron treatment planning.", "content": "In electron treatment planning involving irregular surface contours the technique of shifting standard isodose curves to compensate for air gaps is unsatisfactory for oblique angles of incidence. For electron beams at normal incidence on a flat surface, earlier workers reported that the broad electron beam may be considered as the sum of many narrow beams, and that the standard isodose curves may be reproduced using the data for a narrow beam derived from a collimation experiment. This paper describes a simple alternative experiment to derive the necessary data, which include electrons scattered from the applicator walls. Computed plans using these data for electrons incident at 45 degrees on a flat surface and for incidence on an irregular surface agree with experiment. The method is compatible with, and was designed for, a very limited computing facility.", "contents": "Air-gap correction in electron treatment planning. In electron treatment planning involving irregular surface contours the technique of shifting standard isodose curves to compensate for air gaps is unsatisfactory for oblique angles of incidence. For electron beams at normal incidence on a flat surface, earlier workers reported that the broad electron beam may be considered as the sum of many narrow beams, and that the standard isodose curves may be reproduced using the data for a narrow beam derived from a collimation experiment. This paper describes a simple alternative experiment to derive the necessary data, which include electrons scattered from the applicator walls. Computed plans using these data for electrons incident at 45 degrees on a flat surface and for incidence on an irregular surface agree with experiment. The method is compatible with, and was designed for, a very limited computing facility."}
{"id": "PMID:461527", "title": "The aging intervertebral disk.", "content": "This paper outlines the structure of the intervertebral disk and the process by which altered cellular mechanisms lead to deterioration with age. A reference list indicates current trends in research that attempt to explain the changing nature of the intervertebral disk and its role in spinal disease.", "contents": "The aging intervertebral disk. This paper outlines the structure of the intervertebral disk and the process by which altered cellular mechanisms lead to deterioration with age. A reference list indicates current trends in research that attempt to explain the changing nature of the intervertebral disk and its role in spinal disease."}
{"id": "PMID:461528", "title": "Acute symptomatic disk prolapse. Clinical manifestations and therapeutic considerations.", "content": "Intervertebral disk prolapse is a relatively common cause of acute low back dysfunction. The resultant symptoms and signs can be explained by the pathomechanics and pathophysiology of the intervertebral disk. Clinical manifestations should be used to differentiate the prolapse from more severe disk protrusions and from other types of spinal pathology, for treatment must be pathology-specific. Primary goals of management should include: resolution of the acute problem, restoration of optimal segmental mechanics, restoration of an optimal activity level, prevention of recurrent physical dysfunction, and prevention of chronic pain behavior patterns.", "contents": "Acute symptomatic disk prolapse. Clinical manifestations and therapeutic considerations. Intervertebral disk prolapse is a relatively common cause of acute low back dysfunction. The resultant symptoms and signs can be explained by the pathomechanics and pathophysiology of the intervertebral disk. Clinical manifestations should be used to differentiate the prolapse from more severe disk protrusions and from other types of spinal pathology, for treatment must be pathology-specific. Primary goals of management should include: resolution of the acute problem, restoration of optimal segmental mechanics, restoration of an optimal activity level, prevention of recurrent physical dysfunction, and prevention of chronic pain behavior patterns."}
{"id": "PMID:461580", "title": "[Family therapy--encounters with a therapeutic fashion (author's transl)].", "content": "Following the boom in Group Therapy, the Federal Republic of Germany now experiences a similar boom in Family Thera;y. An attempt is made in the present article to show the stepwise progress from individual to group psychotherapy and finally to family therapy, and to describe and calssify the family therapy approach. The present family therapy boom is considered to be a kind of fashion. In the long run, however, family therapy as such will prove to be an important tool within the therapeutic spectrum. A cause for concern is the high demand made on the training and skill of family therapists. This deters those who had always considered family therapy as an integral part of their work in everyday paractice. This results in a plea for remaining close to actual practical conditions. The article close with recommendations for reading up relevant literature.", "contents": "[Family therapy--encounters with a therapeutic fashion (author's transl)]. Following the boom in Group Therapy, the Federal Republic of Germany now experiences a similar boom in Family Thera;y. An attempt is made in the present article to show the stepwise progress from individual to group psychotherapy and finally to family therapy, and to describe and calssify the family therapy approach. The present family therapy boom is considered to be a kind of fashion. In the long run, however, family therapy as such will prove to be an important tool within the therapeutic spectrum. A cause for concern is the high demand made on the training and skill of family therapists. This deters those who had always considered family therapy as an integral part of their work in everyday paractice. This results in a plea for remaining close to actual practical conditions. The article close with recommendations for reading up relevant literature."}
{"id": "PMID:461581", "title": "[Psychotherapy in anxiety caused by examinations and in learning difficulties (author's transl)].", "content": "Examination neuroses and learning difficulties in college students are almost always amenable to successful psychotherapeutic treatment. The total quota of examination neuroses among students should be about 3% to 4%. The article deals with the factors causing the neurosis, factors which made their appearance either during childhood or adolescence or during college studies only. The psychotherapeutic possibilities are discussed, special attention being paid to the active forms of psychotherapy and an analytically oriented form of group therapy. The good chances of success, which are between 90% and 95%, are underlined.", "contents": "[Psychotherapy in anxiety caused by examinations and in learning difficulties (author's transl)]. Examination neuroses and learning difficulties in college students are almost always amenable to successful psychotherapeutic treatment. The total quota of examination neuroses among students should be about 3% to 4%. The article deals with the factors causing the neurosis, factors which made their appearance either during childhood or adolescence or during college studies only. The psychotherapeutic possibilities are discussed, special attention being paid to the active forms of psychotherapy and an analytically oriented form of group therapy. The good chances of success, which are between 90% and 95%, are underlined."}
{"id": "PMID:461582", "title": "[The future r\u00f4le of psychotherapy in medical care from a medicosocial viewpoint (author's transl)].", "content": "The difficulties of futurological statement about the role of psychotherapy in the medical care system are seen (1) in the problemacy to define the object, (2) to assess the present state of the situation of psychotherapeutic care, (3) to recognize the environmental conditions of the role of psychotherapy is paramount. The futurological hypotheses are centred on the supposition that psychotherapy is more and more tending not be fixed at medical professional roles.", "contents": "[The future r\u00f4le of psychotherapy in medical care from a medicosocial viewpoint (author's transl)]. The difficulties of futurological statement about the role of psychotherapy in the medical care system are seen (1) in the problemacy to define the object, (2) to assess the present state of the situation of psychotherapeutic care, (3) to recognize the environmental conditions of the role of psychotherapy is paramount. The futurological hypotheses are centred on the supposition that psychotherapy is more and more tending not be fixed at medical professional roles."}
{"id": "PMID:461583", "title": "[\"Divided roles\" as a therapeutic game in sociotherapeutic training (author's transl)].", "content": "Basing on an intergative concept in which various aspects of learning and behavioral therapy, depth psychology and communication therapy overlap, the article surveys the possibilities and limitations (inherent risks) of playing \"divided parts\" in theatrical fashion as part of psychiatric training. A section on the basic technique describes how a play according to divided roles is built up in practice. From the viewpoint of a theory of playing divided roles, the functions, aims and a system of typology are discussed.", "contents": "[\"Divided roles\" as a therapeutic game in sociotherapeutic training (author's transl)]. Basing on an intergative concept in which various aspects of learning and behavioral therapy, depth psychology and communication therapy overlap, the article surveys the possibilities and limitations (inherent risks) of playing \"divided parts\" in theatrical fashion as part of psychiatric training. A section on the basic technique describes how a play according to divided roles is built up in practice. From the viewpoint of a theory of playing divided roles, the functions, aims and a system of typology are discussed."}
{"id": "PMID:461585", "title": "Criminal responsibility: an action language approach.", "content": "Traditionally, the propositions of psychiatry are said to be in conflict with those of the law since psychiatry assumes a \"psychic determinism\" and the law assumes freedom of the will (Stone, 1978). An argument always arises over the insanity defense since it bridges psychiatry and the law. But one may hold that there is nothing intrinsic to psychiatry which requires causally stated propositions. By formulating psychiatric propositions in a different form, specifically by adopting the action language of Roy Schafer (1976a), there need be no incompatibility between psychiatric and legal statements about criminal responsibility.", "contents": "Criminal responsibility: an action language approach. Traditionally, the propositions of psychiatry are said to be in conflict with those of the law since psychiatry assumes a \"psychic determinism\" and the law assumes freedom of the will (Stone, 1978). An argument always arises over the insanity defense since it bridges psychiatry and the law. But one may hold that there is nothing intrinsic to psychiatry which requires causally stated propositions. By formulating psychiatric propositions in a different form, specifically by adopting the action language of Roy Schafer (1976a), there need be no incompatibility between psychiatric and legal statements about criminal responsibility."}
{"id": "PMID:461589", "title": "The sense of identity and the role of continuity and confluence.", "content": "THE concept of identity, as it will be used in this paper, corresponds to Erikson's notion of psychosocial identity. It is a sense of \"selfsameness and continuity in time\" and of embeddedness in the \"environment\" (Erikson 1959, 1968). I will describe some of the factors which seem to be essential for the sense of selfsameness, and examine the manner in which continuity is preserved during transitional phases.", "contents": "The sense of identity and the role of continuity and confluence. THE concept of identity, as it will be used in this paper, corresponds to Erikson's notion of psychosocial identity. It is a sense of \"selfsameness and continuity in time\" and of embeddedness in the \"environment\" (Erikson 1959, 1968). I will describe some of the factors which seem to be essential for the sense of selfsameness, and examine the manner in which continuity is preserved during transitional phases."}
{"id": "PMID:461590", "title": "Culture as an internal representation.", "content": "IN ADDITION to many of the more traditional anthropological views of culture, it may be understood as a phenomenon in the minds of individuals. This \"internal representation\" of culture may be seen as the product, viewed from the inside, of the experience through the life cycle of the emotional attachment to a cultural group. Not only does the internal representation of culture play a role as carrier of values, attitudes, and other such phenomena usually  relegated to the realm of the superego, but it also has a significant psychological function in individual development. This may be demonstrated especially with regard to the development and maintenance of a cohesive sense of self. In this brief paper, I would like to present an instance of what may be understood about the development of this internal, experientially based self from the point of view of a psychoanalytically informed anthropological research model.", "contents": "Culture as an internal representation. IN ADDITION to many of the more traditional anthropological views of culture, it may be understood as a phenomenon in the minds of individuals. This \"internal representation\" of culture may be seen as the product, viewed from the inside, of the experience through the life cycle of the emotional attachment to a cultural group. Not only does the internal representation of culture play a role as carrier of values, attitudes, and other such phenomena usually  relegated to the realm of the superego, but it also has a significant psychological function in individual development. This may be demonstrated especially with regard to the development and maintenance of a cohesive sense of self. In this brief paper, I would like to present an instance of what may be understood about the development of this internal, experientially based self from the point of view of a psychoanalytically informed anthropological research model."}
{"id": "PMID:461591", "title": "Hidden stresses in success.", "content": "There are many measures of success. For example, Logan Pearsall Smith, the English essayist, wrote: \"How can they say my life is not a success? Have I not for more than sixty years got enough to eat and escaped being eaten?\" (quoted in Flesch). Conventional measures of success include the attainment of wealth, position, esteem, favor, or eminence, but these things without an accompanying enforcement of self-esteem cause stress--emotional discomfort or strain. Self-esteem, then, is perhaps the essence of the conscious, pleasurable experiencing of success.", "contents": "Hidden stresses in success. There are many measures of success. For example, Logan Pearsall Smith, the English essayist, wrote: \"How can they say my life is not a success? Have I not for more than sixty years got enough to eat and escaped being eaten?\" (quoted in Flesch). Conventional measures of success include the attainment of wealth, position, esteem, favor, or eminence, but these things without an accompanying enforcement of self-esteem cause stress--emotional discomfort or strain. Self-esteem, then, is perhaps the essence of the conscious, pleasurable experiencing of success."}
{"id": "PMID:461592", "title": "The classification of schizophrenic neologisms.", "content": "The use of neologisms has long been considered a symptom of schizophrenic thought disorder. However, a review of current literature and psychiatric textbooks reveals few clinical examples of neologism that may be used for illustrative purposes. A prominent exception is a paper by Forest (1969). The purpose of this communication is to present the case history of a schizophrenic patient, a partial list of his neologistic productions, and a brief analysis of the classification of neologisms.", "contents": "The classification of schizophrenic neologisms. The use of neologisms has long been considered a symptom of schizophrenic thought disorder. However, a review of current literature and psychiatric textbooks reveals few clinical examples of neologism that may be used for illustrative purposes. A prominent exception is a paper by Forest (1969). The purpose of this communication is to present the case history of a schizophrenic patient, a partial list of his neologistic productions, and a brief analysis of the classification of neologisms."}
{"id": "PMID:461593", "title": "The interaction of family therapy and psychodynamic individual therapy in an inpatient setting.", "content": "Although individual psychotherapy is an established treatment modality in psychoanalytically oriented hospitals, family therapy is a relative newcomer. When family therapy is introduced into such an inpatient setting, it raises issues which have not yet been widely discussed. On the one hand there is the question of how inpatient family therapy differs from outpatient family therapy, and what its special characteristics are. This question has only begun to be discussed. On the other hand there is the question of how family therapy relates to individual therapy that is being conducted simultaneously with a member of the same family. In general the individual psychotherapy literature tends to speak as if family therapy did not exist, and the family therapy literature speaks as if individual therapy should be abolished and supplanted by family therapy. Some authors have begun to try to build bridges between the two fields on the level of theory, but almost none have reported on the interaction between the two treatment modalities on a pragmatic level when they are conducted simultaneously (Kugel, 1974).", "contents": "The interaction of family therapy and psychodynamic individual therapy in an inpatient setting. Although individual psychotherapy is an established treatment modality in psychoanalytically oriented hospitals, family therapy is a relative newcomer. When family therapy is introduced into such an inpatient setting, it raises issues which have not yet been widely discussed. On the one hand there is the question of how inpatient family therapy differs from outpatient family therapy, and what its special characteristics are. This question has only begun to be discussed. On the other hand there is the question of how family therapy relates to individual therapy that is being conducted simultaneously with a member of the same family. In general the individual psychotherapy literature tends to speak as if family therapy did not exist, and the family therapy literature speaks as if individual therapy should be abolished and supplanted by family therapy. Some authors have begun to try to build bridges between the two fields on the level of theory, but almost none have reported on the interaction between the two treatment modalities on a pragmatic level when they are conducted simultaneously (Kugel, 1974)."}
{"id": "PMID:461595", "title": "Ward tension and staff leadership in a therapeutic community for hospitalized adolescents.", "content": "Mental health workers on inpatient units spend a great deal of time trying to cope with interpersonal tensions that disrupt ward life. We have focused our attention on two aspects of this problem. The first is clarifying the nature of the social processes that underlie periods of increased tension and conflict on wards. The second is clarifying the kinds of staff leadership required to manage these tensions. We are sure that those who have worked on interactive treatment wards will recognize this situation: for a period of weeks or more there is an uneasy tension; patient cliques form and disruption occurs between cliques and with the staff. Often there is a climax of disruptive behavior, such as a day or weekend when a large number of patients break ward rules. Trouble seems to be contagious. Throughout the period staff members disagree about how to manage the patients and the disruption, and usually this disagreement is tinged with old philosophical or personal differences. No one feels very confident about taking leadership initiatives, and the formal leaders are blamed for various failures and lacks. Eventually, often after a climactic disturbance is resolved, ward life returns to \"normal\" and people feel much better about living and working on the ward. In this paper we review previous work on this kind of ward process and discuss some of the problems involved in conceptualizing it. We report on two period of ward observation that illustrate the sequence from low to high tension and back to relative calm. We then discuss our ideas about the kinds of staff leadership needed to manage different phases of this sequence and the problems of developing and integrating multiple ward leadership roles.", "contents": "Ward tension and staff leadership in a therapeutic community for hospitalized adolescents. Mental health workers on inpatient units spend a great deal of time trying to cope with interpersonal tensions that disrupt ward life. We have focused our attention on two aspects of this problem. The first is clarifying the nature of the social processes that underlie periods of increased tension and conflict on wards. The second is clarifying the kinds of staff leadership required to manage these tensions. We are sure that those who have worked on interactive treatment wards will recognize this situation: for a period of weeks or more there is an uneasy tension; patient cliques form and disruption occurs between cliques and with the staff. Often there is a climax of disruptive behavior, such as a day or weekend when a large number of patients break ward rules. Trouble seems to be contagious. Throughout the period staff members disagree about how to manage the patients and the disruption, and usually this disagreement is tinged with old philosophical or personal differences. No one feels very confident about taking leadership initiatives, and the formal leaders are blamed for various failures and lacks. Eventually, often after a climactic disturbance is resolved, ward life returns to \"normal\" and people feel much better about living and working on the ward. In this paper we review previous work on this kind of ward process and discuss some of the problems involved in conceptualizing it. We report on two period of ward observation that illustrate the sequence from low to high tension and back to relative calm. We then discuss our ideas about the kinds of staff leadership needed to manage different phases of this sequence and the problems of developing and integrating multiple ward leadership roles."}
{"id": "PMID:461596", "title": "Mental health viewed as an ideal.", "content": "The concepts of health and disease have always challenged the human mind. They continue to be frequently discussed with reference to both the body and the mind, but conclusions seem elusive or at best heuristic. With respect to the mind, especially, the challenge seems awesome: When the investigator and what is being investigated are one and the same, impartiality, objectivity, and the other virtues of scientific inquiry appear difficult to assess, much less attain. And yet, not surprisingly, the subject continues to attract investigators (e.g., Kaplan, 1967; Sabshin, 1967; Shakow, 1967; Offer, 1973; Sedgwick, 1973; Bruhn, 1974; Margolis, 1976). Philosophers and physicians alike respond to the subject's appeal--its theoretical interest, the conceptual problem it presents, and its implications for our understanding of ourselves. And those of us who are clincians cannot deny the practical importance of our concepts of mental health and illness: We each daily base decisions on our understanding of these concepts, and even if we cannot provide solid basis for such an understanding, we perhaps owe it to our patients to make explicit what this understanding, our philosophy, is.", "contents": "Mental health viewed as an ideal. The concepts of health and disease have always challenged the human mind. They continue to be frequently discussed with reference to both the body and the mind, but conclusions seem elusive or at best heuristic. With respect to the mind, especially, the challenge seems awesome: When the investigator and what is being investigated are one and the same, impartiality, objectivity, and the other virtues of scientific inquiry appear difficult to assess, much less attain. And yet, not surprisingly, the subject continues to attract investigators (e.g., Kaplan, 1967; Sabshin, 1967; Shakow, 1967; Offer, 1973; Sedgwick, 1973; Bruhn, 1974; Margolis, 1976). Philosophers and physicians alike respond to the subject's appeal--its theoretical interest, the conceptual problem it presents, and its implications for our understanding of ourselves. And those of us who are clincians cannot deny the practical importance of our concepts of mental health and illness: We each daily base decisions on our understanding of these concepts, and even if we cannot provide solid basis for such an understanding, we perhaps owe it to our patients to make explicit what this understanding, our philosophy, is."}
{"id": "PMID:461599", "title": "Development of psychosexual concepts in relation to expressive language performance.", "content": "Cognitive-developmental theory suggests that the learning of gender identity concepts is a sequential process, beginning with the learning of one's own gender, and moving to recognition of the invariance of gender as a human characteristic. Neither clinical nor theoretical literature can fully explain the steps involved. In this study, retarded and nonretarded children were asked to respond to tasks requiring knowledge of core gender identity and gender constancy. When both the retarded and nonretarded children were pooled, a significant interaction was found across all ages between expressive language performance and the development of the psychosexual concepts of core gender identity and gender constancy.", "contents": "Development of psychosexual concepts in relation to expressive language performance. Cognitive-developmental theory suggests that the learning of gender identity concepts is a sequential process, beginning with the learning of one's own gender, and moving to recognition of the invariance of gender as a human characteristic. Neither clinical nor theoretical literature can fully explain the steps involved. In this study, retarded and nonretarded children were asked to respond to tasks requiring knowledge of core gender identity and gender constancy. When both the retarded and nonretarded children were pooled, a significant interaction was found across all ages between expressive language performance and the development of the psychosexual concepts of core gender identity and gender constancy."}
{"id": "PMID:461600", "title": "The relationship between friendship and altruism in preadolescent girls.", "content": "The present study was designed from a Sullivanian theoretical perspective to explore the nature of friendship patterns during preadolescence. Specifically, an attempt was made to replicate with females a previous finding that preadolescent boys involved in a chum relationship exhibited significantly greater levels of altruistic behavior than those without a chum.", "contents": "The relationship between friendship and altruism in preadolescent girls. The present study was designed from a Sullivanian theoretical perspective to explore the nature of friendship patterns during preadolescence. Specifically, an attempt was made to replicate with females a previous finding that preadolescent boys involved in a chum relationship exhibited significantly greater levels of altruistic behavior than those without a chum."}
{"id": "PMID:461691", "title": "Unity and diversity in biochemistry.", "content": "A consideration of A Biochemical Phylogeny of the Protists, by M.A. Ragan and D.J. Chapman (Academic Press, 1978) and Biochemical Adaptation to Environmental Change, ed. by R.M.S. Smellie and J.F. Pennock (the Biochemical Society, 1976), within some thoughts on biochemical evolution.", "contents": "Unity and diversity in biochemistry. A consideration of A Biochemical Phylogeny of the Protists, by M.A. Ragan and D.J. Chapman (Academic Press, 1978) and Biochemical Adaptation to Environmental Change, ed. by R.M.S. Smellie and J.F. Pennock (the Biochemical Society, 1976), within some thoughts on biochemical evolution."}
{"id": "PMID:461719", "title": "The principles of tomographic positioning with particular reference to skull tomography.", "content": "Because the principles of tomography relating to positioning have not been clearly understood it has generally been the practice to employ only basic positions when undertaking tomographic studies. The purpose of this paper is to outline tomographic positioning principles, and to integrate them in a simple positioning system for common usage. This has given rise to the need for a completely new set of technical terms which pertain to positioning for skull tomography. It is hoped to establish an understanding which will enable the radiographer to devise methods of demonstrating structures for which there are no text book references to act as a guide, and to assist the radiologist in the recognition of pseudo-shadows.", "contents": "The principles of tomographic positioning with particular reference to skull tomography. Because the principles of tomography relating to positioning have not been clearly understood it has generally been the practice to employ only basic positions when undertaking tomographic studies. The purpose of this paper is to outline tomographic positioning principles, and to integrate them in a simple positioning system for common usage. This has given rise to the need for a completely new set of technical terms which pertain to positioning for skull tomography. It is hoped to establish an understanding which will enable the radiographer to devise methods of demonstrating structures for which there are no text book references to act as a guide, and to assist the radiologist in the recognition of pseudo-shadows."}
{"id": "PMID:461739", "title": "Ultrasonography of jaundice.", "content": "Ultrasonography is proposed as a valuable first step in the study of the jaundiced patient. Utilizing modern gray scale technology, it is nearly always possible to determine whether or not dilatation of the biliary tree is present. In the presence of diffuse liver disease without obstruction, some assessment of the state of the parenchyma can often be made. In patients with obstruction, its severity and level are usually easy to determine. It may be considerably harder to correctly diagnose the etiology of the obstruction, and more invasive investigations will then be required.", "contents": "Ultrasonography of jaundice. Ultrasonography is proposed as a valuable first step in the study of the jaundiced patient. Utilizing modern gray scale technology, it is nearly always possible to determine whether or not dilatation of the biliary tree is present. In the presence of diffuse liver disease without obstruction, some assessment of the state of the parenchyma can often be made. In patients with obstruction, its severity and level are usually easy to determine. It may be considerably harder to correctly diagnose the etiology of the obstruction, and more invasive investigations will then be required."}
{"id": "PMID:461741", "title": "Clinical comparison of computed tomography and lymphangiography for detection of retroperitoneal lymphadenopathy.", "content": "The advantages of CT scan over lymphangiography are numerous and significant. The accuracy of CT scan was virtually identical to lymphogram for the detection of para-aortic lymph node involvement; however, CT provides a better means of assessing the true extent of disease. In such cases it is possible to determine whether the neoplastic process involves retroperitoneal structures such as the kidneys, ureters, pancreatic region, hilum of the liver, or the major vessels. Moreover, this excellent definition permits accurate follow-up assessment of therapy. Finally, CT is a noninvasive examination and does not require significant technical expertise, and the examination is easily reproducible following any time period from the initial examination. Finally, although we have not specifically discussed biopsy procedures, the CT scan is an excellent means for guiding biopsy procedures even for retroperitoneal abnormalities, perhaps precluding the necessity for laparotomy in order to provide histologic diagnosis of disease (Fig. 11). The disadvantages of CT are several. Of course, the acquisition of CT equipment is expensive and the cost effectiveness has not yet been proven. The most significant disadvantage is its inability to resolve or detect neoplastic disease within normal-sized lymph nodes. This was not a significant problem in our series, because our series contained only a few cases of suspected metastatic disease as opposed to lymph node neoplasms. The advantages of lymphography have been discussed by other authors. Because of the better spatial resolution of lymphograms, it is possible by proper interpretation to distinguish between neoplastic disease and lipogranulomatous changes. In a few cases in our series, this did not prove to be true; however, this may have been due to deficiencies in our interpretations (two cases of lymphoma were called positive for neoplasm, but proved to be lipogranulomatous changes by biopsy). Secondly, surveillance films may be easily and cheaply obtained to detect recurrences of disease. In addition, lymphography also provides the opportunity for biopsy of lymphoma masses, but to date this has only been accomplished by skinny-needle biopsy aspirations and not by large core biopsy techniques. After reviewing the literature and evaluating our data, we believe that several recommendations are appropriate with respect to the roles of computed tomography and lymphography. We agree with the previously stated concept that CT should be used for screening for lymphoma; however, we disagree that lymphograms have a greater advantage over CT in the biopsy-proven cases of lymphoma. Rather we believe that CT is better suited than lymphography for those cases with biopsy proven lymphoma. CT is better able to accurately localize the lymph node masses for surgeons if the surgical approach is desired and better able to define the extent of the disease throughout the abdomen. As a result of this it is capable of providing a better follow-up for therapy...", "contents": "Clinical comparison of computed tomography and lymphangiography for detection of retroperitoneal lymphadenopathy. The advantages of CT scan over lymphangiography are numerous and significant. The accuracy of CT scan was virtually identical to lymphogram for the detection of para-aortic lymph node involvement; however, CT provides a better means of assessing the true extent of disease. In such cases it is possible to determine whether the neoplastic process involves retroperitoneal structures such as the kidneys, ureters, pancreatic region, hilum of the liver, or the major vessels. Moreover, this excellent definition permits accurate follow-up assessment of therapy. Finally, CT is a noninvasive examination and does not require significant technical expertise, and the examination is easily reproducible following any time period from the initial examination. Finally, although we have not specifically discussed biopsy procedures, the CT scan is an excellent means for guiding biopsy procedures even for retroperitoneal abnormalities, perhaps precluding the necessity for laparotomy in order to provide histologic diagnosis of disease (Fig. 11). The disadvantages of CT are several. Of course, the acquisition of CT equipment is expensive and the cost effectiveness has not yet been proven. The most significant disadvantage is its inability to resolve or detect neoplastic disease within normal-sized lymph nodes. This was not a significant problem in our series, because our series contained only a few cases of suspected metastatic disease as opposed to lymph node neoplasms. The advantages of lymphography have been discussed by other authors. Because of the better spatial resolution of lymphograms, it is possible by proper interpretation to distinguish between neoplastic disease and lipogranulomatous changes. In a few cases in our series, this did not prove to be true; however, this may have been due to deficiencies in our interpretations (two cases of lymphoma were called positive for neoplasm, but proved to be lipogranulomatous changes by biopsy). Secondly, surveillance films may be easily and cheaply obtained to detect recurrences of disease. In addition, lymphography also provides the opportunity for biopsy of lymphoma masses, but to date this has only been accomplished by skinny-needle biopsy aspirations and not by large core biopsy techniques. After reviewing the literature and evaluating our data, we believe that several recommendations are appropriate with respect to the roles of computed tomography and lymphography. We agree with the previously stated concept that CT should be used for screening for lymphoma; however, we disagree that lymphograms have a greater advantage over CT in the biopsy-proven cases of lymphoma. Rather we believe that CT is better suited than lymphography for those cases with biopsy proven lymphoma. CT is better able to accurately localize the lymph node masses for surgeons if the surgical approach is desired and better able to define the extent of the disease throughout the abdomen. As a result of this it is capable of providing a better follow-up for therapy..."}
{"id": "PMID:461762", "title": "Fluoroscopic technique for double-contrast knee arthrography.", "content": "The fluoroscopic double-contrast technique of arthrography of the knee is useful in documenting the extent of suspected injury to the knee. It is of particular value in demonstrating small and large lesions involving the menisci, cruciate ligaments, patellar cartilages, as well as the articular cartilages of the femoral condyles and tibial plateaus. This examination is instrumental in the diagnosis of the patient with an atypical history of injury and unusual physical findings. A simple restraint device allows the fluoroscopist complete control over the stress applied to the knee while positioning the patient for filming. Fluoroscopic spot radiographs of excellent contrast and sharp detail can be obtained of each of the various structures, applying the stress needed. When the radiologic technologist takes a little time to become more knowledgeable about the anatomy of the knee jount, and the rationale for the various views, the knee arthrographic examination is easily understood.", "contents": "Fluoroscopic technique for double-contrast knee arthrography. The fluoroscopic double-contrast technique of arthrography of the knee is useful in documenting the extent of suspected injury to the knee. It is of particular value in demonstrating small and large lesions involving the menisci, cruciate ligaments, patellar cartilages, as well as the articular cartilages of the femoral condyles and tibial plateaus. This examination is instrumental in the diagnosis of the patient with an atypical history of injury and unusual physical findings. A simple restraint device allows the fluoroscopist complete control over the stress applied to the knee while positioning the patient for filming. Fluoroscopic spot radiographs of excellent contrast and sharp detail can be obtained of each of the various structures, applying the stress needed. When the radiologic technologist takes a little time to become more knowledgeable about the anatomy of the knee jount, and the rationale for the various views, the knee arthrographic examination is easily understood."}
{"id": "PMID:461763", "title": "A collegiate model within a hospital-based program.", "content": "In the current age of consumerism, educators in radiologic technology must be accountable to provide high level educational experiences for students. The authors address the issue of standardizing and upgrading education by proposing that a collegiate model be developed and implemented with a hospital-based radiography program.", "contents": "A collegiate model within a hospital-based program. In the current age of consumerism, educators in radiologic technology must be accountable to provide high level educational experiences for students. The authors address the issue of standardizing and upgrading education by proposing that a collegiate model be developed and implemented with a hospital-based radiography program."}
{"id": "PMID:461764", "title": "Cervical spin protocol for emergency room use.", "content": "At the University of Alabama Hospitals, we have established a protocol of routine and supplementary projections to provide an unusually thorough and complete evaluation of the patient suspected of harboring certain acute cervical spine injuries. This paper reports an evaluation of a protocol designed for use in the radiographic emergency room. The protocol was designed to provide uniform roentgen assessment of the cervical spine with a minimum number of exposures, ease in radiographing the patient, and strict avoidance of manipulation of the patient. A total of 268 patients were evaluated. Of these patients, 98 (thirty-six per cent) were admitted to the hospital for various reasons and none required additional roentgenograms of the cervical spine for better delineation. In addition, in no instance was a previously undiagnosed cervical spine injury later found.", "contents": "Cervical spin protocol for emergency room use. At the University of Alabama Hospitals, we have established a protocol of routine and supplementary projections to provide an unusually thorough and complete evaluation of the patient suspected of harboring certain acute cervical spine injuries. This paper reports an evaluation of a protocol designed for use in the radiographic emergency room. The protocol was designed to provide uniform roentgen assessment of the cervical spine with a minimum number of exposures, ease in radiographing the patient, and strict avoidance of manipulation of the patient. A total of 268 patients were evaluated. Of these patients, 98 (thirty-six per cent) were admitted to the hospital for various reasons and none required additional roentgenograms of the cervical spine for better delineation. In addition, in no instance was a previously undiagnosed cervical spine injury later found."}
{"id": "PMID:461772", "title": "[Radiology in chest trauma (author's transl)].", "content": "In chest trauma, a routine chest film, preferably in the lateral as well as the frontal projection, is the basic part of the work-up. Occasionally valuable additional methods are fluoroscopy, tomography, bronchography, contrast studies of the GI Tract and angiography and angiocardiography. In 679 chest trauma patients, traffic accidents and falls were the main reason for the trauma. There were 248 fractures; then - in order of frequency - hemopneumothorax (76), lung contusion (58), subcutaneous emphysema (33) cardiac (16) and vascular trauma (12) and damage to other organs. While 20--30% mistakes are made in diagnosing rib fractures in acute trauma, there is high accuracy in the diagnosis of the other injuries. Many cases are shown to demonstrate the value of diagnostic radiology.", "contents": "[Radiology in chest trauma (author's transl)]. In chest trauma, a routine chest film, preferably in the lateral as well as the frontal projection, is the basic part of the work-up. Occasionally valuable additional methods are fluoroscopy, tomography, bronchography, contrast studies of the GI Tract and angiography and angiocardiography. In 679 chest trauma patients, traffic accidents and falls were the main reason for the trauma. There were 248 fractures; then - in order of frequency - hemopneumothorax (76), lung contusion (58), subcutaneous emphysema (33) cardiac (16) and vascular trauma (12) and damage to other organs. While 20--30% mistakes are made in diagnosing rib fractures in acute trauma, there is high accuracy in the diagnosis of the other injuries. Many cases are shown to demonstrate the value of diagnostic radiology."}
{"id": "PMID:461773", "title": "[X-Ray findings of the complications of central venous catheters (author's transl)].", "content": "The increasing use of central venous catheters requires knowledge of possible hazards. Complications such as pneumothorax, hemothorax, hematomas in the chest wall or mediastinum and pulmonary emboli caused by the catheter are easily seen on plain chest films. Cardiac tamponade, perforation of vessels, air emboli and thrombosis have to be considered as a possible cause in case of sudden deterioration of a patient.", "contents": "[X-Ray findings of the complications of central venous catheters (author's transl)]. The increasing use of central venous catheters requires knowledge of possible hazards. Complications such as pneumothorax, hemothorax, hematomas in the chest wall or mediastinum and pulmonary emboli caused by the catheter are easily seen on plain chest films. Cardiac tamponade, perforation of vessels, air emboli and thrombosis have to be considered as a possible cause in case of sudden deterioration of a patient."}
{"id": "PMID:461774", "title": "[Cystic pseudotumors (baker cysts) of the popliteal fossa (author's transl)].", "content": "Report on 38 Baker cysts found in 300 knee arthrographies performed for clinically suspected meniscus lesions. In order not to miss these cysts, fluoroscopy and/or X-ray documentation immediately following the instillation of contrast material and air into the knee joint is mandatory, because these contrast agents tend to disappear fairly rapidly, leaving the lesion poorly visible towards the end of the examination. Furthermore, the clinical significance and the therapeutic aspects of Baker cysts are discussed.", "contents": "[Cystic pseudotumors (baker cysts) of the popliteal fossa (author's transl)]. Report on 38 Baker cysts found in 300 knee arthrographies performed for clinically suspected meniscus lesions. In order not to miss these cysts, fluoroscopy and/or X-ray documentation immediately following the instillation of contrast material and air into the knee joint is mandatory, because these contrast agents tend to disappear fairly rapidly, leaving the lesion poorly visible towards the end of the examination. Furthermore, the clinical significance and the therapeutic aspects of Baker cysts are discussed."}
{"id": "PMID:461775", "title": "[Angiographic findings in hemangiopericytoma of the urinary bladder (author's transl)].", "content": "Case report of a 31 years old white female presenting with a large filling defect in the urinary bladder occupying one half of its lumen. Selective arteriography demonstrated an intramural highly vascular mass, which histologically proved to be a hemangiopericytoma. Angiography in the lateral projection in addition to the frontal projection was necessary to establish the exact location of the mass and thus permit a reasonable differential diagnosis; and angiography also facilitated surgery, of course.", "contents": "[Angiographic findings in hemangiopericytoma of the urinary bladder (author's transl)]. Case report of a 31 years old white female presenting with a large filling defect in the urinary bladder occupying one half of its lumen. Selective arteriography demonstrated an intramural highly vascular mass, which histologically proved to be a hemangiopericytoma. Angiography in the lateral projection in addition to the frontal projection was necessary to establish the exact location of the mass and thus permit a reasonable differential diagnosis; and angiography also facilitated surgery, of course."}
{"id": "PMID:461776", "title": "[Comparison of screens and screen-film-systems (author's transl)].", "content": "Important details are to be payed attention in comparison of different scrreens resp. screen-film-systems: 1. Physical characteristics of different groups of luminescent materials: f.i. calcium tung-state, rare-earths compounds, double halogenides. - 2. Different types of screens: highest details up to highest speed intensifying screens, have to be defined more specifically and differentiated against to each other too. - 3. Besides intensification, resolution has to be included into consideration since one of these dates alone does not allow any statement on the total function of a screen or a screen-film-system. - 4. The technical methodological conditions of apparatuses, object and its positioning have to be defined, f.i. X-ray quality, distances, grid, and in automatically controlled exposition, if necessary, position of ionization chamber as well as absorption of cassetts and screen. - 5. Considering these points gradation curves have to include the whole necessary or interesting diagnostic range. - 6. Due to functional correlation between intensification and resolution, the resolution has to be taken in consideration due to application; its interdependence of density and object (f.i. scattered radiation) is often not taken enough in consideration.", "contents": "[Comparison of screens and screen-film-systems (author's transl)]. Important details are to be payed attention in comparison of different scrreens resp. screen-film-systems: 1. Physical characteristics of different groups of luminescent materials: f.i. calcium tung-state, rare-earths compounds, double halogenides. - 2. Different types of screens: highest details up to highest speed intensifying screens, have to be defined more specifically and differentiated against to each other too. - 3. Besides intensification, resolution has to be included into consideration since one of these dates alone does not allow any statement on the total function of a screen or a screen-film-system. - 4. The technical methodological conditions of apparatuses, object and its positioning have to be defined, f.i. X-ray quality, distances, grid, and in automatically controlled exposition, if necessary, position of ionization chamber as well as absorption of cassetts and screen. - 5. Considering these points gradation curves have to include the whole necessary or interesting diagnostic range. - 6. Due to functional correlation between intensification and resolution, the resolution has to be taken in consideration due to application; its interdependence of density and object (f.i. scattered radiation) is often not taken enough in consideration."}
{"id": "PMID:461777", "title": "Images, image quality and observer performance: new horizons in radiology lecture.", "content": "Technical and diagnostic image quality are distinguished and the limitations of three methods currently used for assessing diagnostic image quality are discussed. They are evaluations based on individual clinical experience, measurement of diagnostic performance, and physical measurements made on images or imaging systems. Finally, a psychophysical approach to image quality evaluation is presented and its potential value discussed.", "contents": "Images, image quality and observer performance: new horizons in radiology lecture. Technical and diagnostic image quality are distinguished and the limitations of three methods currently used for assessing diagnostic image quality are discussed. They are evaluations based on individual clinical experience, measurement of diagnostic performance, and physical measurements made on images or imaging systems. Finally, a psychophysical approach to image quality evaluation is presented and its potential value discussed."}
{"id": "PMID:461778", "title": "Breast cancer screening with mammography: a population-based, randomized trial with mammography as the only screening mode.", "content": "A population-based, randomized breast cancer screening project was undertaken using mammography alone. Of 17,447 invited women aged 50-69, 12,765 (73%) attended the screening. On the basis of the screening films, malignancy was suspected in 405 women (3.2%) who were recalled for complete mammography. Additional films showed that the suspicion of malignancy was false in 194 women. The remaining 211 women (1.7%) were referred for clinical and cytological examination. Of these, 159 had surgery. Breast cancer was proved in 97 women, corresponding to a prevalence rate of 7.6/1000. Fifty-three (55%) of the carcinomas were either in situ or invasive with a diameter of less than or equal to 1 cm. Axillary metastases were found in 19 patients (19.6%). Cancers detected at screening were significantly less advanced than those in the control group. There was a remarkably high frequency of tubular carcinoma among cancers detected at screening.", "contents": "Breast cancer screening with mammography: a population-based, randomized trial with mammography as the only screening mode. A population-based, randomized breast cancer screening project was undertaken using mammography alone. Of 17,447 invited women aged 50-69, 12,765 (73%) attended the screening. On the basis of the screening films, malignancy was suspected in 405 women (3.2%) who were recalled for complete mammography. Additional films showed that the suspicion of malignancy was false in 194 women. The remaining 211 women (1.7%) were referred for clinical and cytological examination. Of these, 159 had surgery. Breast cancer was proved in 97 women, corresponding to a prevalence rate of 7.6/1000. Fifty-three (55%) of the carcinomas were either in situ or invasive with a diameter of less than or equal to 1 cm. Axillary metastases were found in 19 patients (19.6%). Cancers detected at screening were significantly less advanced than those in the control group. There was a remarkably high frequency of tubular carcinoma among cancers detected at screening."}
{"id": "PMID:461779", "title": "Errors of interpretation as elicited by a quality audit of an emergency radiology facility.", "content": "A process-oriented quality care audit was performed in a large metropolitan hospital emergency radiology facility with an annual volume of over 50,000 examinations. One aspect of the audit dealt with errors found among interpretations by radiology residents, the initial interpreters of x-ray studies. Misinterpretations were identified by staff radiologists, who checked all examinations and countersigned the reports. Error rates were correlated with duration of training and were separated as to significance and whether the errors were false-negative (omission) or false-positive (commission). The false-positive to false-negative ratio was 27:73% which is in agreement with previous studies. For all cases of errors, the significance of change in interpretation was high in 20%, moderate in 29% and low in 51%. The effect of inadequate clinical history on the rate and significance of interpretation errors was also determined. When clinical information was inadequate, the significance was high in 27%, moderate in 40% and low in 33%.", "contents": "Errors of interpretation as elicited by a quality audit of an emergency radiology facility. A process-oriented quality care audit was performed in a large metropolitan hospital emergency radiology facility with an annual volume of over 50,000 examinations. One aspect of the audit dealt with errors found among interpretations by radiology residents, the initial interpreters of x-ray studies. Misinterpretations were identified by staff radiologists, who checked all examinations and countersigned the reports. Error rates were correlated with duration of training and were separated as to significance and whether the errors were false-negative (omission) or false-positive (commission). The false-positive to false-negative ratio was 27:73% which is in agreement with previous studies. For all cases of errors, the significance of change in interpretation was high in 20%, moderate in 29% and low in 51%. The effect of inadequate clinical history on the rate and significance of interpretation errors was also determined. When clinical information was inadequate, the significance was high in 27%, moderate in 40% and low in 33%."}
{"id": "PMID:461780", "title": "Pleural telangiectasia and absence of a pulmonary artery.", "content": "The radiological features of absence of a pulmonary artery are often characteristic enough to allow a diagnosis based on the plain radiograph. They include a small hemithorax, ipsilateral displacement of the mediastinum, absence of the corresponding pulmonary artery, and reticular parenchymal densities, generally attributed to bronchial collateral vessels. Six cases are reported; 3 patients had peripheral, hazy, confluent densities and pleural thickening. Aortography disclosed extensive intercostal and subdiaphragmatic transpleural collaterals, which formed telangiectatic networks within the pleura of the oligemic lungs, corresponding to the areas of increased density on the plain radiographs. The appearance of these transpleural collaterals is helpful in diagnosis. Pleural telangiectasia in association with prolonged pulmonary oligemia of any cause indicates significant transpleural collaterals.", "contents": "Pleural telangiectasia and absence of a pulmonary artery. The radiological features of absence of a pulmonary artery are often characteristic enough to allow a diagnosis based on the plain radiograph. They include a small hemithorax, ipsilateral displacement of the mediastinum, absence of the corresponding pulmonary artery, and reticular parenchymal densities, generally attributed to bronchial collateral vessels. Six cases are reported; 3 patients had peripheral, hazy, confluent densities and pleural thickening. Aortography disclosed extensive intercostal and subdiaphragmatic transpleural collaterals, which formed telangiectatic networks within the pleura of the oligemic lungs, corresponding to the areas of increased density on the plain radiographs. The appearance of these transpleural collaterals is helpful in diagnosis. Pleural telangiectasia in association with prolonged pulmonary oligemia of any cause indicates significant transpleural collaterals."}
{"id": "PMID:461781", "title": "Transluminal angioplasty of the iliac, femoral, and popliteal arteries.", "content": "The recently developed Gr\u00fcntzig balloon dilatation catheter has facilitated the performance of transluminal angioplasty. The authors used this catheter in 35 arteries supplying the lower extremities in 27 patients. Immediate relief of symptoms (claudication and rest pain) occurred in 30 vessels (86%) in 23 patients. The procedure was well tolerated by all patients. Over 90% of initially successful dilatations were patent at 3 to 10 months.", "contents": "Transluminal angioplasty of the iliac, femoral, and popliteal arteries. The recently developed Gr\u00fcntzig balloon dilatation catheter has facilitated the performance of transluminal angioplasty. The authors used this catheter in 35 arteries supplying the lower extremities in 27 patients. Immediate relief of symptoms (claudication and rest pain) occurred in 30 vessels (86%) in 23 patients. The procedure was well tolerated by all patients. Over 90% of initially successful dilatations were patent at 3 to 10 months."}
{"id": "PMID:461782", "title": "Hemodynamic significance of iliac artery stenosis: pressure measurements during angiography.", "content": "Peak systolic pressure gradients were obtained before and after vasodilatation in 42 patients (50 limbs) with arteriographic iliac artery stenosis of questionable significance. Patients were divided into three groups according to per cent narrowing of the iliac artery. Pressure gradients across the site of stenosis tended to be significant in patients with greater than 75% stenosis (greater than or equal to 20 mm Hg) but not in patients with less than 50% stenosis; patients in the middle group (50-75% stenosis) demonstrated a wide variance. Thus the arteriogram is not an accurate indicator of hemodynamically significant lesions, particularly in patients with 50-75% stenosis where pressure measurements are of greatest value. Variations in the aortic and femoral artery systolic peak pressure occurred following vasodilatation, indicating the importance of simultaneous pressure recording.", "contents": "Hemodynamic significance of iliac artery stenosis: pressure measurements during angiography. Peak systolic pressure gradients were obtained before and after vasodilatation in 42 patients (50 limbs) with arteriographic iliac artery stenosis of questionable significance. Patients were divided into three groups according to per cent narrowing of the iliac artery. Pressure gradients across the site of stenosis tended to be significant in patients with greater than 75% stenosis (greater than or equal to 20 mm Hg) but not in patients with less than 50% stenosis; patients in the middle group (50-75% stenosis) demonstrated a wide variance. Thus the arteriogram is not an accurate indicator of hemodynamically significant lesions, particularly in patients with 50-75% stenosis where pressure measurements are of greatest value. Variations in the aortic and femoral artery systolic peak pressure occurred following vasodilatation, indicating the importance of simultaneous pressure recording."}
{"id": "PMID:461783", "title": "Arteria magna revisited.", "content": "Arteria magna has been described pathologically in the literature as a peculiar and unique form of atherosclerosis. The authors offer an alternative hypothesis, based on results of angiographic studies with pathologic correlation in 18 patients with arteria magna. The findings indicate that this vascular entity might be more accurately described as a loss of the medial elastica with subsequent vessel enlargement both in length and diameter.", "contents": "Arteria magna revisited. Arteria magna has been described pathologically in the literature as a peculiar and unique form of atherosclerosis. The authors offer an alternative hypothesis, based on results of angiographic studies with pathologic correlation in 18 patients with arteria magna. The findings indicate that this vascular entity might be more accurately described as a loss of the medial elastica with subsequent vessel enlargement both in length and diameter."}
{"id": "PMID:461784", "title": "\"Mini\" Gianturco stainless steel coils for transcatheter vascular occlusion.", "content": "Small stainless steel coils have been developed for transcatheter vascular occlusion procedures. The coils can be passed through a 5-French polyethylene catheter, extending the clinical applications of coil occlusion procedures for small and/or tortuous vessels. Two cases describing the use of these coils are reported.", "contents": "\"Mini\" Gianturco stainless steel coils for transcatheter vascular occlusion. Small stainless steel coils have been developed for transcatheter vascular occlusion procedures. The coils can be passed through a 5-French polyethylene catheter, extending the clinical applications of coil occlusion procedures for small and/or tortuous vessels. Two cases describing the use of these coils are reported."}
{"id": "PMID:461785", "title": "The angiographic features of extraabdominal desmoid tumors.", "content": "Extraabdominal desmoid tumors are nonencapsulated locally invasive neoplasms of fibrous tissue. The angiographic features include arterial stretching, neovascularity, and tumor staining (4 of 6 cases in this series). Although benign, these tumors are difficult to cure because they tend to recur locally.", "contents": "The angiographic features of extraabdominal desmoid tumors. Extraabdominal desmoid tumors are nonencapsulated locally invasive neoplasms of fibrous tissue. The angiographic features include arterial stretching, neovascularity, and tumor staining (4 of 6 cases in this series). Although benign, these tumors are difficult to cure because they tend to recur locally."}
{"id": "PMID:461786", "title": "Urticaria of the colon.", "content": "The clinical manifestations of colonic urticaria vary, depending on the presence of associated abnormalities. A case of an association between colonic urticaria and cecal volvulus is described.", "contents": "Urticaria of the colon. The clinical manifestations of colonic urticaria vary, depending on the presence of associated abnormalities. A case of an association between colonic urticaria and cecal volvulus is described."}
{"id": "PMID:461787", "title": "Thermographic diagnosis of deep venous thrombosis: anatomically based diagnostic criteria.", "content": "Deep venous thrombosis is associated with increased heat emission from involved muscle groups and deviation of blood flow from the deep to the superficial venous system. Both of these features can be documented by thermography. An appreciation of the muscular and venous anatomy of the lower limb allows recognition of predictable thermographic images reflecting involvement of various muscle groups in the leg. It is expected that these anatomically based criteria will improve the diagnostic accuracy of thermography and offer a noninvasive, easily repeatable modality of examination for the diagnosis of deep venous thrombosis.", "contents": "Thermographic diagnosis of deep venous thrombosis: anatomically based diagnostic criteria. Deep venous thrombosis is associated with increased heat emission from involved muscle groups and deviation of blood flow from the deep to the superficial venous system. Both of these features can be documented by thermography. An appreciation of the muscular and venous anatomy of the lower limb allows recognition of predictable thermographic images reflecting involvement of various muscle groups in the leg. It is expected that these anatomically based criteria will improve the diagnostic accuracy of thermography and offer a noninvasive, easily repeatable modality of examination for the diagnosis of deep venous thrombosis."}
{"id": "PMID:461788", "title": "Effects of radiopaque contrast media on the microcirculation of the rabbit omentum.", "content": "Histamine concentration in plasma was measured and the microvasculature studied after injections of radiographic contrast materials and hypertonic NaCl solutions. Only 8 ml/kg of contrast material caused a prolonged decrease in systemic and microvascular pressure and a drop in erythrocyte velocity. Concomitantly, a significant increase in plasma histamine concentration was observed after two minutes. Signs of cellular defects and constriction of arterioles were noted after several minutes. These findings suggest that the hyperosmolality of the radiopaque materials tested could initiate the microhemodynamic reactions but is not responsible for cellular and endothelial damage in the microcirculation of the rabbit omentum.", "contents": "Effects of radiopaque contrast media on the microcirculation of the rabbit omentum. Histamine concentration in plasma was measured and the microvasculature studied after injections of radiographic contrast materials and hypertonic NaCl solutions. Only 8 ml/kg of contrast material caused a prolonged decrease in systemic and microvascular pressure and a drop in erythrocyte velocity. Concomitantly, a significant increase in plasma histamine concentration was observed after two minutes. Signs of cellular defects and constriction of arterioles were noted after several minutes. These findings suggest that the hyperosmolality of the radiopaque materials tested could initiate the microhemodynamic reactions but is not responsible for cellular and endothelial damage in the microcirculation of the rabbit omentum."}
{"id": "PMID:461789", "title": "Inhibition of platelet aggregation by contrast media.", "content": "The effect of 30% Urografin on platelet aggregation induced by adenosine diphosphate (ADP), epinephrine, and arachidonic acid was examined. In vivo and in vitro experiments in 10 nondiabetic subjects and in vitro experiments in 7 diabetic-uremic patients showed a statistically significant decrease in platelet aggregation 20 minutes after in vitro incubation or in vivo infusion with Urografin. Urografin concentration was about 2.4-2.5%. The possibility of bleeding induced by contrast media and the connection with acute renal failure in diabetic-uremic patients are discussed.", "contents": "Inhibition of platelet aggregation by contrast media. The effect of 30% Urografin on platelet aggregation induced by adenosine diphosphate (ADP), epinephrine, and arachidonic acid was examined. In vivo and in vitro experiments in 10 nondiabetic subjects and in vitro experiments in 7 diabetic-uremic patients showed a statistically significant decrease in platelet aggregation 20 minutes after in vitro incubation or in vivo infusion with Urografin. Urografin concentration was about 2.4-2.5%. The possibility of bleeding induced by contrast media and the connection with acute renal failure in diabetic-uremic patients are discussed."}
{"id": "PMID:461790", "title": "Long-term effects of bacteriuria on the urinary tract in schoolgirls.", "content": "In order to examine the effects of bacteriuria on renal growth and scarring, 208 schoolgirls aged 5 to 12 years, in whom bacteriuria was detected during a screening program, were followed for 4 years. Of these, 110 were treated. Excretory urography and micturating cystography were performed at the start and end of the study period. Remission of ureteric reflux was equally common in both treated and untreated children. Renal scarring progressed in 12, and diminution in renal growth without scarring occurred in 9 others; treatment did not affect either of these events. A significant association existed between reflux and scarring. No new scars developed in previously unscarred kidneys. Difficulties in assessing normal renal growth and progression of scarring are discussed.", "contents": "Long-term effects of bacteriuria on the urinary tract in schoolgirls. In order to examine the effects of bacteriuria on renal growth and scarring, 208 schoolgirls aged 5 to 12 years, in whom bacteriuria was detected during a screening program, were followed for 4 years. Of these, 110 were treated. Excretory urography and micturating cystography were performed at the start and end of the study period. Remission of ureteric reflux was equally common in both treated and untreated children. Renal scarring progressed in 12, and diminution in renal growth without scarring occurred in 9 others; treatment did not affect either of these events. A significant association existed between reflux and scarring. No new scars developed in previously unscarred kidneys. Difficulties in assessing normal renal growth and progression of scarring are discussed."}
{"id": "PMID:461791", "title": "Acute ureteral obstruction secondary to bullous cystitis of the trigone: report of 2 cases.", "content": "Two boys presented with acute bullous cystitis limited to the trigone and periureteral zone and producing marked but transient acute ureteral obstruction. One patient was anuric and likely would have died without intervention. Cystoscopy in both cases showed localized bullous edema of the bladder mucosa; biopsy showed acute inflammation, mucosal edema, and lymphangiectasis. Radiological findings included distal ureteral obstruction and numerous nodular filling defects in the bladder, caused by the edema. The etiology of the cystitis could not be determined in either case.", "contents": "Acute ureteral obstruction secondary to bullous cystitis of the trigone: report of 2 cases. Two boys presented with acute bullous cystitis limited to the trigone and periureteral zone and producing marked but transient acute ureteral obstruction. One patient was anuric and likely would have died without intervention. Cystoscopy in both cases showed localized bullous edema of the bladder mucosa; biopsy showed acute inflammation, mucosal edema, and lymphangiectasis. Radiological findings included distal ureteral obstruction and numerous nodular filling defects in the bladder, caused by the edema. The etiology of the cystitis could not be determined in either case."}
{"id": "PMID:461792", "title": "Duration of voluntary apnea in children.", "content": "Results of breathholding were recorded in 50 children prior to excretory urography to help determine the optimal sectional imaging technique according to age and expected ability to suspend respiration during examination.", "contents": "Duration of voluntary apnea in children. Results of breathholding were recorded in 50 children prior to excretory urography to help determine the optimal sectional imaging technique according to age and expected ability to suspend respiration during examination."}
{"id": "PMID:461793", "title": "Posterior cerebral artery occlusion: clinical, computed tomographic, and angiographic correlation.", "content": "The distribution of low-density areas on computed tomography (CT) suggested occlusions in the proximal half of the circummesencephalic portion in 38 patients with posterior cerebral artery (PCA) occlusion. Correlation between clinical, CT, and angiographic findings in 24 cases showed that occlusion was most common in the crural segment. Clinical manifestations and infarction extension varied widely among proximal occlusions. In cases with good collateral filling, the infarction was restricted to the thalamus; in those with poor filling, it involved most of the PCA's territory, and hemorrhagic transformation occasionally ensued. Discrepancies between findings were ascribed to dislodging of emboli or thrombi, recanalization, and transient obscuration of the infarction on CT.", "contents": "Posterior cerebral artery occlusion: clinical, computed tomographic, and angiographic correlation. The distribution of low-density areas on computed tomography (CT) suggested occlusions in the proximal half of the circummesencephalic portion in 38 patients with posterior cerebral artery (PCA) occlusion. Correlation between clinical, CT, and angiographic findings in 24 cases showed that occlusion was most common in the crural segment. Clinical manifestations and infarction extension varied widely among proximal occlusions. In cases with good collateral filling, the infarction was restricted to the thalamus; in those with poor filling, it involved most of the PCA's territory, and hemorrhagic transformation occasionally ensued. Discrepancies between findings were ascribed to dislodging of emboli or thrombi, recanalization, and transient obscuration of the infarction on CT."}
{"id": "PMID:461794", "title": "Angiographically undetected ulceration of the carotid bifurcation as a cause of embolic stroke.", "content": "The accuracy of angiographic diagnosis of carotid artery ulceration was evaluated. Of those carotid bifurcations showing ulceration at surgery, 60% were diagnosed as having ulcers at angiography. Half of the remaining ulcers occurred in smooth, benign-appearing plaques and were too small to be seen at angiography. An incorrect angiographic diagnosis of ulceration was made in 17 of 50 carotid arteries; in most cases this was due to the presence of a subintimal hematoma in the wall of the artery.", "contents": "Angiographically undetected ulceration of the carotid bifurcation as a cause of embolic stroke. The accuracy of angiographic diagnosis of carotid artery ulceration was evaluated. Of those carotid bifurcations showing ulceration at surgery, 60% were diagnosed as having ulcers at angiography. Half of the remaining ulcers occurred in smooth, benign-appearing plaques and were too small to be seen at angiography. An incorrect angiographic diagnosis of ulceration was made in 17 of 50 carotid arteries; in most cases this was due to the presence of a subintimal hematoma in the wall of the artery."}
{"id": "PMID:461795", "title": "Venous occlusions of the cavernous area--a complication of crushing fractures of the sphenoid bone.", "content": "Four patients who sustained crushing fractures of the sphenoidal bone had angiographic evidence of venous occlusions of the cavernous area. Signs depended upon the type of basal venous and cavernous anatomy. The diagnosis can be made by demonstrating abrupt cutoff of veins in the cavernous region, preferred retrograde flow of the anterior cavernous sinus, direct venous hemorrhage, and venous stasis.", "contents": "Venous occlusions of the cavernous area--a complication of crushing fractures of the sphenoid bone. Four patients who sustained crushing fractures of the sphenoidal bone had angiographic evidence of venous occlusions of the cavernous area. Signs depended upon the type of basal venous and cavernous anatomy. The diagnosis can be made by demonstrating abrupt cutoff of veins in the cavernous region, preferred retrograde flow of the anterior cavernous sinus, direct venous hemorrhage, and venous stasis."}
{"id": "PMID:461796", "title": "Intrasellar carotid anastomosis simulating pituitary microadenoma.", "content": "Arterial communication between the cavernous segments of the carotid arteries associated with agenesis of the unilateral internal carotid artery is a rare anomaly. Two cases of this anomalous artery with an abnormal sella turcica due to the intrasellar course are reported.", "contents": "Intrasellar carotid anastomosis simulating pituitary microadenoma. Arterial communication between the cavernous segments of the carotid arteries associated with agenesis of the unilateral internal carotid artery is a rare anomaly. Two cases of this anomalous artery with an abnormal sella turcica due to the intrasellar course are reported."}
{"id": "PMID:461797", "title": "Computed tomography of the cruciate ligaments.", "content": "Computed tomography was used to delineate the cruciate ligaments of the knee joint in 31 patients. Optimal positioning for demonstrating the length and body of the anterior and posterior cruciate ligaments is described and the CT, arthrographic, surgical, and clinical findings are correlated. The potential and limitations of CT and its role in combination with double-contrast arthrography in the clinical setting are discussed.", "contents": "Computed tomography of the cruciate ligaments. Computed tomography was used to delineate the cruciate ligaments of the knee joint in 31 patients. Optimal positioning for demonstrating the length and body of the anterior and posterior cruciate ligaments is described and the CT, arthrographic, surgical, and clinical findings are correlated. The potential and limitations of CT and its role in combination with double-contrast arthrography in the clinical setting are discussed."}
{"id": "PMID:461798", "title": "Preoperative determination of abdominal extent of renal cell carcinoma by computed tomography.", "content": "CT scans were assessed for ability to detect abdominal extent of tumor in 17 patients with renal cell carcinoma. Computed tomography has a unique advantage over other imaging modalities in that it can distinguish between tumors confined to Gerota's fascia and those which show contiguous spread, invading adjacent structures such as muscles, liver and the periaortic and pericaval regions. CT can also detect regional lymph node metastases and tumor extension into the renal vein and inferior vena cava. Preoperative assessment of tumor extent helps to determine patient prognosis, surgical approach and whether transcatheter tumor embolization should be used.", "contents": "Preoperative determination of abdominal extent of renal cell carcinoma by computed tomography. CT scans were assessed for ability to detect abdominal extent of tumor in 17 patients with renal cell carcinoma. Computed tomography has a unique advantage over other imaging modalities in that it can distinguish between tumors confined to Gerota's fascia and those which show contiguous spread, invading adjacent structures such as muscles, liver and the periaortic and pericaval regions. CT can also detect regional lymph node metastases and tumor extension into the renal vein and inferior vena cava. Preoperative assessment of tumor extent helps to determine patient prognosis, surgical approach and whether transcatheter tumor embolization should be used."}
{"id": "PMID:461799", "title": "Nonuniform attenuation in computed tomography study of the cirrhotic liver.", "content": "Diffuse hepatocellular processes are not well imaged by most radiologic modalities. At present, CT has not added significantly to the evaluation of hepatocellular disease, particularly in the cirrhotic liver. The CT patterns of cirrhosis previously described are reviewed, and the pathophysiology of cirrhosis is discussed. In 3 cases, a pattern was seen in cirrhotic livers characterized by nonuniform attenuation with varied response to administration of intravenous contrast agents. With further evaluation of fatty infiltration and cirrhosis, CT may prove helpful in understanding these disease processes.", "contents": "Nonuniform attenuation in computed tomography study of the cirrhotic liver. Diffuse hepatocellular processes are not well imaged by most radiologic modalities. At present, CT has not added significantly to the evaluation of hepatocellular disease, particularly in the cirrhotic liver. The CT patterns of cirrhosis previously described are reviewed, and the pathophysiology of cirrhosis is discussed. In 3 cases, a pattern was seen in cirrhotic livers characterized by nonuniform attenuation with varied response to administration of intravenous contrast agents. With further evaluation of fatty infiltration and cirrhosis, CT may prove helpful in understanding these disease processes."}
{"id": "PMID:461800", "title": "Gray-scale ultrasound detection of carotid body tumors. Report of 2 cases.", "content": "Ultrasonography may be useful as a screening test for carotid body tumors before arteriography. Such tumors are seen as solid, well-circumscribed, weakly echogenic masses located at the carotid bifurcation.", "contents": "Gray-scale ultrasound detection of carotid body tumors. Report of 2 cases. Ultrasonography may be useful as a screening test for carotid body tumors before arteriography. Such tumors are seen as solid, well-circumscribed, weakly echogenic masses located at the carotid bifurcation."}
{"id": "PMID:461801", "title": "Limitations in the echocardiographic assessment of aortic root dimensions in the presence of aortic valve disease.", "content": "Aortic root M-mode echocardiograms were evaluated in 79 patients who had aortic valve replacement. Echocardiographic measurements of aortic root dimensions correlated poorly with surgical estimates. Inclusion of a portion of the sinuses of Valsalva in the former set of estimates probably accounts for this discrepancy.", "contents": "Limitations in the echocardiographic assessment of aortic root dimensions in the presence of aortic valve disease. Aortic root M-mode echocardiograms were evaluated in 79 patients who had aortic valve replacement. Echocardiographic measurements of aortic root dimensions correlated poorly with surgical estimates. Inclusion of a portion of the sinuses of Valsalva in the former set of estimates probably accounts for this discrepancy."}
{"id": "PMID:461802", "title": "Ultrasound and computed tomography of the abdomen in the staging and management of testicular carcinoma.", "content": "Ultrasound and computed tomography (CT) are ideal for imaging enlarged retroperitoneal lymph nodes. The authors evaluated 290 ultrasound and 188 CT examinations in 136 patients with testicular cancer. Although interpretation of CT was more reproducible than that of ultrasound, there was no significant difference in accuracy of enlarged retroperitoneal lymph node detection between the two modalities. Accuracy improved when both examinations were used and interpreted in combination by a single radiologist. Accuracy rates for ultrasound and CT were equal to those reported for lymphangiography. Lymphangiography and excretory urography are no longer routinely indicated in testicular cancer patients.", "contents": "Ultrasound and computed tomography of the abdomen in the staging and management of testicular carcinoma. Ultrasound and computed tomography (CT) are ideal for imaging enlarged retroperitoneal lymph nodes. The authors evaluated 290 ultrasound and 188 CT examinations in 136 patients with testicular cancer. Although interpretation of CT was more reproducible than that of ultrasound, there was no significant difference in accuracy of enlarged retroperitoneal lymph node detection between the two modalities. Accuracy improved when both examinations were used and interpreted in combination by a single radiologist. Accuracy rates for ultrasound and CT were equal to those reported for lymphangiography. Lymphangiography and excretory urography are no longer routinely indicated in testicular cancer patients."}
{"id": "PMID:461803", "title": "Sensitivity of radionuclide brain scan and computed tomography in early detection of viral meningoencephalitis.", "content": "The sensitivity of radionuclide imaging and computed tomography (CT) was evaluated in 25 patients for early detection of viral meningoencephalitis. Diagnosis was based on clinical evidence, cerebrospinal fluid (CSF) studies, electroencephalography (EEG) and radionuclide imaging. Computed tomography with contrast enhancement was performed within four days after onset of neurological signs or symptoms in 23 patients; no significant findings such as low-absorption abnormalities, mass effect or abnormal enhancement were seen. Radionuclide imaging demonstrated a sensitivity of 90% in the detection of viral meningoencephalitis; the temporal lobe was most commonly involved in patients with herpes encephalitis. Radionuclide imaging should be considered as the first diagnostic procedure in suspected early viral meningoencephalitis.", "contents": "Sensitivity of radionuclide brain scan and computed tomography in early detection of viral meningoencephalitis. The sensitivity of radionuclide imaging and computed tomography (CT) was evaluated in 25 patients for early detection of viral meningoencephalitis. Diagnosis was based on clinical evidence, cerebrospinal fluid (CSF) studies, electroencephalography (EEG) and radionuclide imaging. Computed tomography with contrast enhancement was performed within four days after onset of neurological signs or symptoms in 23 patients; no significant findings such as low-absorption abnormalities, mass effect or abnormal enhancement were seen. Radionuclide imaging demonstrated a sensitivity of 90% in the detection of viral meningoencephalitis; the temporal lobe was most commonly involved in patients with herpes encephalitis. Radionuclide imaging should be considered as the first diagnostic procedure in suspected early viral meningoencephalitis."}
{"id": "PMID:461804", "title": "Bone stress: a radionuclide imaging perspective.", "content": "Thirty-five college athletes with lower leg pain underwent radiography and radionuclide studies to rule out a stress fracture. Their asymptomatic extremities and 13 pain-free athletes served as controls. Four main patterns were observed: (a) sharply marginated scintigraphic abnormalities and positive radiographs; (b) sharply marginated scintigraphic abnormalities and negative radiographs; (c) ill-defined scintigraphic abnormalities and negative radiographs; and (d) negative radionuclide images and negative radiographs. Since the patients with the first two patterns were otherwise identical medically, the authors feel that this scintigraphic appearance is characteristic of bone stress in the appropriate clinical setting, regardless of the radiographic findings. A schema is proposed to explain the occurrence of positive radionuclide images and negative radiographs in the same patient, using a broad conceptual approach to the problem of bone stress.", "contents": "Bone stress: a radionuclide imaging perspective. Thirty-five college athletes with lower leg pain underwent radiography and radionuclide studies to rule out a stress fracture. Their asymptomatic extremities and 13 pain-free athletes served as controls. Four main patterns were observed: (a) sharply marginated scintigraphic abnormalities and positive radiographs; (b) sharply marginated scintigraphic abnormalities and negative radiographs; (c) ill-defined scintigraphic abnormalities and negative radiographs; and (d) negative radionuclide images and negative radiographs. Since the patients with the first two patterns were otherwise identical medically, the authors feel that this scintigraphic appearance is characteristic of bone stress in the appropriate clinical setting, regardless of the radiographic findings. A schema is proposed to explain the occurrence of positive radionuclide images and negative radiographs in the same patient, using a broad conceptual approach to the problem of bone stress."}
{"id": "PMID:461805", "title": "Percutaneous biopsy following positive bone scans.", "content": "When a bone scan reveals an abnormal radionuclide accumulation and the cause is uncertain, bone biopsy is sometimes necessary. One hundred thirty biopsies have been performed in our institution with a percutaneous needle guided by fluoroscopy. This procedure is safe and effective.", "contents": "Percutaneous biopsy following positive bone scans. When a bone scan reveals an abnormal radionuclide accumulation and the cause is uncertain, bone biopsy is sometimes necessary. One hundred thirty biopsies have been performed in our institution with a percutaneous needle guided by fluoroscopy. This procedure is safe and effective."}
{"id": "PMID:461806", "title": "Altered growth and development of lower teeth in children receiving mantle therapy.", "content": "The improvement in survival and local control rates in children with neoplasms in the head and neck region can be expected to demonstrate increased iatrogenic adverse effects of treatment. The authors report on mandibular growth and developmental abnormalities in 3 children with Hodgkin disease treated by external beam megavoltage therapy. Radiations to the mandible during mantle field therapy were measured and found to be significant. Possible methods of dose reduction are discussed.", "contents": "Altered growth and development of lower teeth in children receiving mantle therapy. The improvement in survival and local control rates in children with neoplasms in the head and neck region can be expected to demonstrate increased iatrogenic adverse effects of treatment. The authors report on mandibular growth and developmental abnormalities in 3 children with Hodgkin disease treated by external beam megavoltage therapy. Radiations to the mandible during mantle field therapy were measured and found to be significant. Possible methods of dose reduction are discussed."}
{"id": "PMID:461807", "title": "Pathological findings after preoperative irradiation for carcinoma of the urinary bladder.", "content": "Pathological descriptions of specimens obtained in 17 patients with urinary bladder carcinoma both at biopsy before preoperative irradiation and after surgery are presented. No positive lymph nodes were found in 16 patients who had lymphadenectomy at the time of surgery. No tumor was found postoperatively in 58.8% of patients and 82.3% had a reduction in tumor stage.", "contents": "Pathological findings after preoperative irradiation for carcinoma of the urinary bladder. Pathological descriptions of specimens obtained in 17 patients with urinary bladder carcinoma both at biopsy before preoperative irradiation and after surgery are presented. No positive lymph nodes were found in 16 patients who had lymphadenectomy at the time of surgery. No tumor was found postoperatively in 58.8% of patients and 82.3% had a reduction in tumor stage."}
{"id": "PMID:461808", "title": "A study of mammographic exposure and detail visibility using three systems: Xerox 125, Min-R, and Xonics XERG.", "content": "A breast phantom of novel design has been used to measure visibility of simulated calcific and soft-tissue fibrillar details in mammography, as well as to determine the roentgen exposure vs. depth. Exposure data were combined with a model of the breast as compressed during mammography to compute the mean exposure to the ductal parenchyma (MDE). Three different imaging systems were compared over a wide range of x-ray beam energies and breast characteristics. \"Dosage\" criteria other than the MDE are discussed.", "contents": "A study of mammographic exposure and detail visibility using three systems: Xerox 125, Min-R, and Xonics XERG. A breast phantom of novel design has been used to measure visibility of simulated calcific and soft-tissue fibrillar details in mammography, as well as to determine the roentgen exposure vs. depth. Exposure data were combined with a model of the breast as compressed during mammography to compute the mean exposure to the ductal parenchyma (MDE). Three different imaging systems were compared over a wide range of x-ray beam energies and breast characteristics. \"Dosage\" criteria other than the MDE are discussed."}
{"id": "PMID:461809", "title": "Semiautomated methods for quantitating CSF volume on cranial computed tomography.", "content": "Two methods for quantitating cerebrospinal fluid (CSF) volume in each hemicranium and on the total section on cranial computed tomography are described and compared. Both analyses utilize information input by the user, but differ in their treatment of volume averaging. The second analysis (Automated Section Information-I) measured partially volumed CSF more sensitively. Potential inaccuracies due to technical artifacts are summarized.", "contents": "Semiautomated methods for quantitating CSF volume on cranial computed tomography. Two methods for quantitating cerebrospinal fluid (CSF) volume in each hemicranium and on the total section on cranial computed tomography are described and compared. Both analyses utilize information input by the user, but differ in their treatment of volume averaging. The second analysis (Automated Section Information-I) measured partially volumed CSF more sensitively. Potential inaccuracies due to technical artifacts are summarized."}
{"id": "PMID:461810", "title": "Preparation of 52Fe and its use in a 52Fe/52mMn generator.", "content": "Clinically useful quantities of 52Fe have been prepared at the Brookhaven Linac Isotope Producer (BLIP) by bombarding manganese or nickel targets with medium energy protons. After chemical separation, 52Fe is loaded onto a generator column from which carrier-free 52Mn is eluted. The 52mMn generator is potentially useful in emission computed tomography.", "contents": "Preparation of 52Fe and its use in a 52Fe/52mMn generator. Clinically useful quantities of 52Fe have been prepared at the Brookhaven Linac Isotope Producer (BLIP) by bombarding manganese or nickel targets with medium energy protons. After chemical separation, 52Fe is loaded onto a generator column from which carrier-free 52Mn is eluted. The 52mMn generator is potentially useful in emission computed tomography."}
{"id": "PMID:461811", "title": "Factors predisposing to radiation-related small-bowel damage.", "content": "From 1970 through 1977, 92 patients with ovarian cancer received 20 Gy (2,000 rad) to the abdomen, followed by 30 Gy (3,000 rad) to the pelvis. Small-bowel obstruction developed in 7 (7.6%). The number of previous laparotomies, thin physique, and hypertension were significantly associated with complications. Two patients receiving isoniazid and 2 receiving Premarin had enteric complications. Pre-existing vascular damage may potentiate radiation damage to the small bowel.", "contents": "Factors predisposing to radiation-related small-bowel damage. From 1970 through 1977, 92 patients with ovarian cancer received 20 Gy (2,000 rad) to the abdomen, followed by 30 Gy (3,000 rad) to the pelvis. Small-bowel obstruction developed in 7 (7.6%). The number of previous laparotomies, thin physique, and hypertension were significantly associated with complications. Two patients receiving isoniazid and 2 receiving Premarin had enteric complications. Pre-existing vascular damage may potentiate radiation damage to the small bowel."}
{"id": "PMID:461812", "title": "The medial adductor approach to arthrography of the hip in children.", "content": "In small infants and children, the medial or adductor approach for arthrography of the hip has several advantages over direct anterior insertion of the needle into the joint capsule, primarily the fact that extravasated contrast material runs along the adductor area and is not superimposed on the hip joint.", "contents": "The medial adductor approach to arthrography of the hip in children. In small infants and children, the medial or adductor approach for arthrography of the hip has several advantages over direct anterior insertion of the needle into the joint capsule, primarily the fact that extravasated contrast material runs along the adductor area and is not superimposed on the hip joint."}
{"id": "PMID:461813", "title": "Xeroradiographic localization of implanted 125I seeds.", "content": "In many instances implanted 125I seeds are shown better by xeroradiography than by conventional film-cassette combinations.", "contents": "Xeroradiographic localization of implanted 125I seeds. In many instances implanted 125I seeds are shown better by xeroradiography than by conventional film-cassette combinations."}
{"id": "PMID:461814", "title": "Wire localization of nonpalpable breast lesions.", "content": "The authors describe a method of localizing nonpalpable breast lesions using a needle and wire. It allows accurate localization without worrying about shifting of the wire before or during biopsy.", "contents": "Wire localization of nonpalpable breast lesions. The authors describe a method of localizing nonpalpable breast lesions using a needle and wire. It allows accurate localization without worrying about shifting of the wire before or during biopsy."}
{"id": "PMID:461815", "title": "An adjustable sterile lead apron for radiation protection during angiography.", "content": "A simple sterile lead apron can be mounted directly on any vertical-beam image-intensifier housing and readjusted by the angiographer to shield himself from scatter during fluoroscopy and cineangiocardiography, even if the image intensifier is titled in the longitudinal plane. Properly placed, the apron effectively reduces exposed due to scatter.", "contents": "An adjustable sterile lead apron for radiation protection during angiography. A simple sterile lead apron can be mounted directly on any vertical-beam image-intensifier housing and readjusted by the angiographer to shield himself from scatter during fluoroscopy and cineangiocardiography, even if the image intensifier is titled in the longitudinal plane. Properly placed, the apron effectively reduces exposed due to scatter."}
{"id": "PMID:461816", "title": "Immobilization of intra-oral interstitial radioactive-needle implants using an intravenous catheter.", "content": "A method of immobilizing intra-oral needle implants using an intravenous catheter is described. A loop formed by the catheter immobilizes the tongue and serves as an anchor for the needles. The technique is simple, flexible, and well tolerated.", "contents": "Immobilization of intra-oral interstitial radioactive-needle implants using an intravenous catheter. A method of immobilizing intra-oral needle implants using an intravenous catheter is described. A loop formed by the catheter immobilizes the tongue and serves as an anchor for the needles. The technique is simple, flexible, and well tolerated."}
{"id": "PMID:461819", "title": "The effects of trans trans methyl linoleate on the concentration of prostaglandins and their precursors in rat.", "content": "Four groups of weanling male rats were fed a diet containing hydrogenated coconut oil (Treatment A); 9-trans, 12-trans linoleate (trans linoleate, (Treatment B); an equal mixture of 9-cis, 12-cis linoleate (cis linoleate) and trans linoleate (Treatment C); and cis linoleate (Treatment D); respectively for 12 weeks. The level of dietary fat was 11% of calories. Only trace amount of eicosatrienoic acid (C20:3w6) was detected in tissues, (liver, platelets) of rats in Treatments A and B. The level of C20:3w6 in platelet lipids of Treatments C and D was 0.1 and 0.33% respectively. The level of arachidonic acid in rats on Treatments A, B, C and D was 2.0, 1.4, 14.1 and 17.6% for platelet lipids, respectively. The serum levels of prostaglandin PGE1 for Treatments A, B, C and D were 1.10 +/- 0.24, 0.22 +/- 0.02, 3.51 +/- 0.58 and 5.69 +/- 0.59 ng/ml, respectively and 2.19 +/- 0.85, 0.15 +/- 0.03, 11.64 +/- 2.63 and 24.89 +/- 4.35 ng/ml for PGE2, respectively. Thus feeding trans linoleate to rats apparently caused decreased biosynthesis of PGs resulting from decreased levels of precursor acids. This was apparently due to the inhibition of conversion of cis linoleate to longer chain polyunsaturated fatty acids. The results indicate that the availability of precursor acids is one limiting factor in PG biosynthesis in rats, and small differences in the level of precursor acids, affected by dietary trans fatty acids may cause large variations in amounts of PGs synthesized.", "contents": "The effects of trans trans methyl linoleate on the concentration of prostaglandins and their precursors in rat. Four groups of weanling male rats were fed a diet containing hydrogenated coconut oil (Treatment A); 9-trans, 12-trans linoleate (trans linoleate, (Treatment B); an equal mixture of 9-cis, 12-cis linoleate (cis linoleate) and trans linoleate (Treatment C); and cis linoleate (Treatment D); respectively for 12 weeks. The level of dietary fat was 11% of calories. Only trace amount of eicosatrienoic acid (C20:3w6) was detected in tissues, (liver, platelets) of rats in Treatments A and B. The level of C20:3w6 in platelet lipids of Treatments C and D was 0.1 and 0.33% respectively. The level of arachidonic acid in rats on Treatments A, B, C and D was 2.0, 1.4, 14.1 and 17.6% for platelet lipids, respectively. The serum levels of prostaglandin PGE1 for Treatments A, B, C and D were 1.10 +/- 0.24, 0.22 +/- 0.02, 3.51 +/- 0.58 and 5.69 +/- 0.59 ng/ml, respectively and 2.19 +/- 0.85, 0.15 +/- 0.03, 11.64 +/- 2.63 and 24.89 +/- 4.35 ng/ml for PGE2, respectively. Thus feeding trans linoleate to rats apparently caused decreased biosynthesis of PGs resulting from decreased levels of precursor acids. This was apparently due to the inhibition of conversion of cis linoleate to longer chain polyunsaturated fatty acids. The results indicate that the availability of precursor acids is one limiting factor in PG biosynthesis in rats, and small differences in the level of precursor acids, affected by dietary trans fatty acids may cause large variations in amounts of PGs synthesized."}
{"id": "PMID:461820", "title": "Desensitization of prostaglandin-activated platelet adenylate cyclase.", "content": "Prostaglandin D2 (PGD2) is one of several prostaglandins that can inhibit platelet aggregation and activate adenylate cyclase. Platelets were exposed to varying concentrations of PGD2 washed, and the adenylate cyclase response to prostaglandins, epinephrine, and sodium fluoride determined. Incubating platelets with 5 x 10(-5) M PGD2 for 2 hr resulted in a 45% decrease in PGD2 activation of adenylate cyclase and a 25% decrease in stimulation by PGE1. Fluoride activation (7-fold) epinephrine inhibition (30%) and basal enzyme activity were unchanged by exposure of the platelets to PGD2. Desensitization was concentration dependent, with loss of enzyme activity first noted when platelets were incubated with 10(-7) M PGD2. Enzyme sensitivity could be partially restored when desensitized platelets were washed free of PGD2 and incubated in buffer for 2 hr; complete resensitization required incubation for 24 hr in plasma. Regulation of prostaglandin sensitive platelet adenylate cyclase could be of importance in mediating the response of platelets to aggregating agents.", "contents": "Desensitization of prostaglandin-activated platelet adenylate cyclase. Prostaglandin D2 (PGD2) is one of several prostaglandins that can inhibit platelet aggregation and activate adenylate cyclase. Platelets were exposed to varying concentrations of PGD2 washed, and the adenylate cyclase response to prostaglandins, epinephrine, and sodium fluoride determined. Incubating platelets with 5 x 10(-5) M PGD2 for 2 hr resulted in a 45% decrease in PGD2 activation of adenylate cyclase and a 25% decrease in stimulation by PGE1. Fluoride activation (7-fold) epinephrine inhibition (30%) and basal enzyme activity were unchanged by exposure of the platelets to PGD2. Desensitization was concentration dependent, with loss of enzyme activity first noted when platelets were incubated with 10(-7) M PGD2. Enzyme sensitivity could be partially restored when desensitized platelets were washed free of PGD2 and incubated in buffer for 2 hr; complete resensitization required incubation for 24 hr in plasma. Regulation of prostaglandin sensitive platelet adenylate cyclase could be of importance in mediating the response of platelets to aggregating agents."}
{"id": "PMID:461821", "title": "Plasma catecholamines in man are not influenced by the inhibition of prostaglandin synthesis.", "content": "Indomethacin has been reported to potentiate the release of noradrenaline from sympathetic nerve endings in vitro and to increase urinary noradrenaline excretion in rats. We have studied the influence of indomethacin on plasma catecholamine levels in 10 normal men, using measurement of plasma renin activity (PRA) as an index of the pharmacodynamic effect of indomethacin. Both in the supine and standing positions indomethacin failed to alter the plasma concentrations of noradrenaline, adrenaline or dopamine, while PRA was markedly suppressed. It is concluded that in the intact human indomethacin does not influence catecholamine concentrations.", "contents": "Plasma catecholamines in man are not influenced by the inhibition of prostaglandin synthesis. Indomethacin has been reported to potentiate the release of noradrenaline from sympathetic nerve endings in vitro and to increase urinary noradrenaline excretion in rats. We have studied the influence of indomethacin on plasma catecholamine levels in 10 normal men, using measurement of plasma renin activity (PRA) as an index of the pharmacodynamic effect of indomethacin. Both in the supine and standing positions indomethacin failed to alter the plasma concentrations of noradrenaline, adrenaline or dopamine, while PRA was markedly suppressed. It is concluded that in the intact human indomethacin does not influence catecholamine concentrations."}
{"id": "PMID:461822", "title": "Interaction of prostaglandins with adrenal microsomal cytochrome P-450 in the guinea pig.", "content": "Studies were carried out to investigate the effects of prostaglandins (PG) in vitro on adrenal microsomal steroid and drug metabolism in the guinea pig. The addition of PGE1, PGE2, PGA1, PGF1 alpha or PGF2 alpha to isolated adrenal microsomes produced typical type I difference spectra. The sizes of the spectra (delta A385-420) produced by prostaglandins were smaller than those produced by various steroids including progesterone, 17-hydroxyprogesterone and 11 beta-hydroxyprogesterone. However, the affinities of prostaglandins and steroids for adrenal microsomal cytochrome P-450, as estimated by the spectral dissociation constants, were similar. Prior addition of prostaglandins to isolated adrenal microsomes did not affect steroid binding to cytochrome P-450 or the rate of steroid 21-hydroxylation. In contrast, prostaglandins inhibited adrenal metabolism of ethylmorphine and diminished the magnitude of the ethylmorphine-induced spectral change in adrenal microsomes. The results indicate that prostaglandins inhibit adrenal drug metabolism by interfering with substrate binding to cytochrome P-450. Since 21-hydroxylation was unaffected by PG, different cytochrome P-450 moieties are probably involved in adrenal drug and steroid metabolism.", "contents": "Interaction of prostaglandins with adrenal microsomal cytochrome P-450 in the guinea pig. Studies were carried out to investigate the effects of prostaglandins (PG) in vitro on adrenal microsomal steroid and drug metabolism in the guinea pig. The addition of PGE1, PGE2, PGA1, PGF1 alpha or PGF2 alpha to isolated adrenal microsomes produced typical type I difference spectra. The sizes of the spectra (delta A385-420) produced by prostaglandins were smaller than those produced by various steroids including progesterone, 17-hydroxyprogesterone and 11 beta-hydroxyprogesterone. However, the affinities of prostaglandins and steroids for adrenal microsomal cytochrome P-450, as estimated by the spectral dissociation constants, were similar. Prior addition of prostaglandins to isolated adrenal microsomes did not affect steroid binding to cytochrome P-450 or the rate of steroid 21-hydroxylation. In contrast, prostaglandins inhibited adrenal metabolism of ethylmorphine and diminished the magnitude of the ethylmorphine-induced spectral change in adrenal microsomes. The results indicate that prostaglandins inhibit adrenal drug metabolism by interfering with substrate binding to cytochrome P-450. Since 21-hydroxylation was unaffected by PG, different cytochrome P-450 moieties are probably involved in adrenal drug and steroid metabolism."}
{"id": "PMID:461823", "title": "Uterine vein prostaglandin levels in late pregnant dogs.", "content": "We studied the uterine venous plasma concentrations of prostaglandins E2, F2 alpha, 15 keto 13,14 dihydro E2 and 15 keto 13,14 dihydro F2 alpha in late pregnant dogs in order to evaluate the rates of production and metabolism of prostaglandin E2 and F2 alpha in pregnancy in vivo. We used a very specific and sensitive gas chromatography-mass spectrometry assay to measure these prostaglandins. The uterine venous concentrations of prostaglandin E2 and 15 keto 13,14 dihydro E2 were 1.35 +/- .27 ng/ml and 1.89 +/- .37 ng/ml, respectively; however, we could not find any prostaglandin F2 alpha and very little of its plasma metabolite in uterine venous plasma. Since uterine microsomes can generate prostaglandin F2 alpha and E2 from endoperoxides, prostaglandin F2 alpha production in vivo must be regulated through an enzymatic step after endoperoxide formation. Prostaglandin E2 is produced by pregnant canine uterus in quantities high enough to have a biological effect in late pregnancy; however, prostaglandin F2 alpha does not appear to play a role at this stage of pregnancy.", "contents": "Uterine vein prostaglandin levels in late pregnant dogs. We studied the uterine venous plasma concentrations of prostaglandins E2, F2 alpha, 15 keto 13,14 dihydro E2 and 15 keto 13,14 dihydro F2 alpha in late pregnant dogs in order to evaluate the rates of production and metabolism of prostaglandin E2 and F2 alpha in pregnancy in vivo. We used a very specific and sensitive gas chromatography-mass spectrometry assay to measure these prostaglandins. The uterine venous concentrations of prostaglandin E2 and 15 keto 13,14 dihydro E2 were 1.35 +/- .27 ng/ml and 1.89 +/- .37 ng/ml, respectively; however, we could not find any prostaglandin F2 alpha and very little of its plasma metabolite in uterine venous plasma. Since uterine microsomes can generate prostaglandin F2 alpha and E2 from endoperoxides, prostaglandin F2 alpha production in vivo must be regulated through an enzymatic step after endoperoxide formation. Prostaglandin E2 is produced by pregnant canine uterus in quantities high enough to have a biological effect in late pregnancy; however, prostaglandin F2 alpha does not appear to play a role at this stage of pregnancy."}
{"id": "PMID:461824", "title": "Role of endogenous prostaglandins in regulating the tone of opossum lower esophageal sphincter in vivo.", "content": "The role of prostaglandins in maintenance of basal myogenic tone of the lower esophageal sphincter (LES) of opossum has been studied in vivo. Intra-arterial infusion of arachidonic acid decreased LES tone, and this was inhibited by intravenous indomethacin (IDM) or intra-arterial 5,8,11,14-eicosatetraynoic acid (ETA). Alone these drugs did not reduce LES tone except transiently. In addition they did not affect relaxation of the LES to distention of a balloon located proximal to it or inhibit the \"off\" contractions of esophageal body and LES pressure which followed balloon deflation. Spontaneous oscillations of LES pressure were increased with IDM. Thus prostaglandin synthesis plays no essential role in maintenance of resting LES tone or in functioning of non-adrenergic inhibitory nerves in the esophagus in vivo. Endogenous inhibitory prostaglandins might reduce LES tone if synthesized in increased amounts.", "contents": "Role of endogenous prostaglandins in regulating the tone of opossum lower esophageal sphincter in vivo. The role of prostaglandins in maintenance of basal myogenic tone of the lower esophageal sphincter (LES) of opossum has been studied in vivo. Intra-arterial infusion of arachidonic acid decreased LES tone, and this was inhibited by intravenous indomethacin (IDM) or intra-arterial 5,8,11,14-eicosatetraynoic acid (ETA). Alone these drugs did not reduce LES tone except transiently. In addition they did not affect relaxation of the LES to distention of a balloon located proximal to it or inhibit the \"off\" contractions of esophageal body and LES pressure which followed balloon deflation. Spontaneous oscillations of LES pressure were increased with IDM. Thus prostaglandin synthesis plays no essential role in maintenance of resting LES tone or in functioning of non-adrenergic inhibitory nerves in the esophagus in vivo. Endogenous inhibitory prostaglandins might reduce LES tone if synthesized in increased amounts."}
{"id": "PMID:461825", "title": "Effects of prostaglandins E1 and E2 on activity in laryngeal and pharyngeal afferent fibers.", "content": "Prostaglandins (PG) E1 and E2 were applied topically to the receptive fields of feline laryngeal and pharyngeal sensory receptors, while action potentials were recorded from single - or few-fiber preparations of the superior laryngeal nerve. When initially dissolved in ethanol, PGs stimulated these sensory receptors. If ethanol was not used as a solvent for the PGs they did not stimulate the sensory receptors. Similarly, local application of dilute (0.025%, v/v) solutions of ethanol alone excited the receptors, whereas phosphate buffer alone did not. Thus PGE1 and PGE2 do not themselves stimulate sensory receptors in the larynx and pharynx. These findings suggest that irritant properties of PGEs on upper airways are attributable to the ethanol used as a solvent.", "contents": "Effects of prostaglandins E1 and E2 on activity in laryngeal and pharyngeal afferent fibers. Prostaglandins (PG) E1 and E2 were applied topically to the receptive fields of feline laryngeal and pharyngeal sensory receptors, while action potentials were recorded from single - or few-fiber preparations of the superior laryngeal nerve. When initially dissolved in ethanol, PGs stimulated these sensory receptors. If ethanol was not used as a solvent for the PGs they did not stimulate the sensory receptors. Similarly, local application of dilute (0.025%, v/v) solutions of ethanol alone excited the receptors, whereas phosphate buffer alone did not. Thus PGE1 and PGE2 do not themselves stimulate sensory receptors in the larynx and pharynx. These findings suggest that irritant properties of PGEs on upper airways are attributable to the ethanol used as a solvent."}
{"id": "PMID:461842", "title": "[Radiological study of the hand of patients undergoing periodic hemodialysis. Possibilities and limitations of the method as an index of the evolution of the uremic disease].", "content": "The periodic X-ray examination of the hand in 50 patients in hemodialysis shows a good correlation between the radiologic findings and bone bioptic patterns. The method had instead a lesser interest in following the evolution of vascular and metastatic calcification.", "contents": "[Radiological study of the hand of patients undergoing periodic hemodialysis. Possibilities and limitations of the method as an index of the evolution of the uremic disease]. The periodic X-ray examination of the hand in 50 patients in hemodialysis shows a good correlation between the radiologic findings and bone bioptic patterns. The method had instead a lesser interest in following the evolution of vascular and metastatic calcification."}
{"id": "PMID:461844", "title": "[Pulmonary throboembolism: radiological examination without contrast media. (Findings in 70 cases)].", "content": "The results are reported of radiologic investigations carried out without contrast medium in 70 selected patients with pulmonary thromboembolisms. The frequency of different signs is reported. Attention is stressed of the following data: 1) value of some semeiologic findings both hilar (hilar amputation, arterial dilation) and parenchimal (increased translucency, hypoperfusion), mainly compared with previous radiographic examinations; 2) frequency of atelectasis with its various findings; 3) significance of the evolution referred both to the infarction and the thromboembolic pattern.", "contents": "[Pulmonary throboembolism: radiological examination without contrast media. (Findings in 70 cases)]. The results are reported of radiologic investigations carried out without contrast medium in 70 selected patients with pulmonary thromboembolisms. The frequency of different signs is reported. Attention is stressed of the following data: 1) value of some semeiologic findings both hilar (hilar amputation, arterial dilation) and parenchimal (increased translucency, hypoperfusion), mainly compared with previous radiographic examinations; 2) frequency of atelectasis with its various findings; 3) significance of the evolution referred both to the infarction and the thromboembolic pattern."}
{"id": "PMID:461845", "title": "[Radiologic evaluation of bronchial asthma (author's transl)].", "content": "The physiopathologic and anatomic findings of bronchial asthma are shortly reviewed. A description follows of the radiologic patterns based on personal experience. Only seldom the radiologic investigations provide the possibility to reach the correct diagnosis of bronchial asthma. But they always complete the clinical evaluation and allow the demonstration of the numerous and frequent complications which influence the evolution and prognosis of the disease.", "contents": "[Radiologic evaluation of bronchial asthma (author's transl)]. The physiopathologic and anatomic findings of bronchial asthma are shortly reviewed. A description follows of the radiologic patterns based on personal experience. Only seldom the radiologic investigations provide the possibility to reach the correct diagnosis of bronchial asthma. But they always complete the clinical evaluation and allow the demonstration of the numerous and frequent complications which influence the evolution and prognosis of the disease."}
{"id": "PMID:461846", "title": "[Correlations between nephrotomography and arteriography in the diagnosis of renal mass lesions (author's transl)].", "content": "A series of 325 patients with renal mass lesions were examined by means nephrotomography during the years 1973-76; 85 of them (26%) underwent angiographic investigations. The basic findings observed with nephrotomography are described followed by the criteria which have suggested the necessity of the arteriographic examination. The analysis of the correlations between the two methods, shows the criteria which may limit the use of renal arteriography.", "contents": "[Correlations between nephrotomography and arteriography in the diagnosis of renal mass lesions (author's transl)]. A series of 325 patients with renal mass lesions were examined by means nephrotomography during the years 1973-76; 85 of them (26%) underwent angiographic investigations. The basic findings observed with nephrotomography are described followed by the criteria which have suggested the necessity of the arteriographic examination. The analysis of the correlations between the two methods, shows the criteria which may limit the use of renal arteriography."}
{"id": "PMID:461853", "title": "[Prilocaine as an hypotensive ocular agent (author's transl)].", "content": "The purpose of the present investigation was to determine the action of prilocaine as hypotensive ocular agent. Prilocaine in doses of 2.5 mg/kg, 5 mg/kg and 10 mg/kg was administered intravenously in dogs anesthetized with sodium pentobarbital (30 mg/kg). The experiments show that prilocaine when used i.v. in doses of 2.5 mg/kg and 5 mg/kg causes ocular hypotension in dogs, whereas doses of 10 mg/kg i.v. produce this effect only when artificial respiration is employed, thus preventing the increase of the pCO2 in the blood.", "contents": "[Prilocaine as an hypotensive ocular agent (author's transl)]. The purpose of the present investigation was to determine the action of prilocaine as hypotensive ocular agent. Prilocaine in doses of 2.5 mg/kg, 5 mg/kg and 10 mg/kg was administered intravenously in dogs anesthetized with sodium pentobarbital (30 mg/kg). The experiments show that prilocaine when used i.v. in doses of 2.5 mg/kg and 5 mg/kg causes ocular hypotension in dogs, whereas doses of 10 mg/kg i.v. produce this effect only when artificial respiration is employed, thus preventing the increase of the pCO2 in the blood."}
{"id": "PMID:461847", "title": "[Radiological findings in traumatic lesions of the achilles tendon (author's transl)].", "content": "Xeroradiography fits particularly well in the study of the soft tissues and given better results than traditional radiographs. Traumatic pathology of the Achille's tendon has been studied. Usually the lateral projection both of the affected and the unaffected sides is enough. In some cases xeroradiographs are made with the foot both in hyperflexion and in hyperextension. Cases with partial or complete fracture of the Achille's tendon are described as well as cases with nodular tendinitis or tendinosis, as a sequela of traumatic lesions of the tendon. Finally the findings of the surgical reconstruction of the tendon are discribed.", "contents": "[Radiological findings in traumatic lesions of the achilles tendon (author's transl)]. Xeroradiography fits particularly well in the study of the soft tissues and given better results than traditional radiographs. Traumatic pathology of the Achille's tendon has been studied. Usually the lateral projection both of the affected and the unaffected sides is enough. In some cases xeroradiographs are made with the foot both in hyperflexion and in hyperextension. Cases with partial or complete fracture of the Achille's tendon are described as well as cases with nodular tendinitis or tendinosis, as a sequela of traumatic lesions of the tendon. Finally the findings of the surgical reconstruction of the tendon are discribed."}
{"id": "PMID:461848", "title": "[Aneurysms of the renal artery (author's transl)].", "content": "After a review of the literature concerning the frequency, aetiology, roentgenologic diagnosis and treatment of the aneurysm of the renal arteries, the author reports 2 cases of renal aneurysm with hypertension causing segmental reduction of renal blood flow. The value of selective renal arteriography and aortography is stressed.", "contents": "[Aneurysms of the renal artery (author's transl)]. After a review of the literature concerning the frequency, aetiology, roentgenologic diagnosis and treatment of the aneurysm of the renal arteries, the author reports 2 cases of renal aneurysm with hypertension causing segmental reduction of renal blood flow. The value of selective renal arteriography and aortography is stressed."}
{"id": "PMID:461854", "title": "[Reactions of subcutaneous connective tissue and incised wounds to strips of furacin gauze. Histologic study (author's transl].", "content": "Strips of gauze and furacin gauze were implanted into the subcutaneous space and employed as dressing of dorsal incisions of albino rats. The animals were sacrificed after 24 hours, 2, 5, and 7 post-operative days. The pieces were processed for histological examination. It is possible to conclude that the furacin gauze provokes more severe inflammatory reactions and does not favour the connective neoformation and the epithelization. The furacin gauze should be utilized only as germicide aim.", "contents": "[Reactions of subcutaneous connective tissue and incised wounds to strips of furacin gauze. Histologic study (author's transl]. Strips of gauze and furacin gauze were implanted into the subcutaneous space and employed as dressing of dorsal incisions of albino rats. The animals were sacrificed after 24 hours, 2, 5, and 7 post-operative days. The pieces were processed for histological examination. It is possible to conclude that the furacin gauze provokes more severe inflammatory reactions and does not favour the connective neoformation and the epithelization. The furacin gauze should be utilized only as germicide aim."}
{"id": "PMID:461850", "title": "[Results of radiotherapy in tumors of the rhinopharynx].", "content": "Cumulative 5 years survival following radiotherapy for carcinoma of the nasopharynx does not exceed 30 divided by 35%. Better survival rates are reported in patients with primary lesion of limited extent (T1) or without neck lymph node involvement (N0): 5 years survival 50 divided by 60%. Reports from the most recent series and a review of 159 cases treated at National Cancer Institute of Milano indicate the prognostic significance of the histological type. The 5 years survival rate for \"well differentiated carcinoma\" ranges from 10 to 34%, in comparison with 25 divided by 50% for the \"undifferentiated\" type. Local recurrence and invasion of the base of the skull are seen more frequently in the \"well differentiated\" group while the \"undifferentiated carcinoma\" shows a stronger tendency to distant metastases (30 divided 35%). On the base of these data a more selected therapeutic approach is suggested.", "contents": "[Results of radiotherapy in tumors of the rhinopharynx]. Cumulative 5 years survival following radiotherapy for carcinoma of the nasopharynx does not exceed 30 divided by 35%. Better survival rates are reported in patients with primary lesion of limited extent (T1) or without neck lymph node involvement (N0): 5 years survival 50 divided by 60%. Reports from the most recent series and a review of 159 cases treated at National Cancer Institute of Milano indicate the prognostic significance of the histological type. The 5 years survival rate for \"well differentiated carcinoma\" ranges from 10 to 34%, in comparison with 25 divided by 50% for the \"undifferentiated\" type. Local recurrence and invasion of the base of the skull are seen more frequently in the \"well differentiated\" group while the \"undifferentiated carcinoma\" shows a stronger tendency to distant metastases (30 divided 35%). On the base of these data a more selected therapeutic approach is suggested."}
{"id": "PMID:461855", "title": "[Sodium transport and potassium excretion by the salivary ducts of the dog's submandibular gland (author's transl)].", "content": "In order to study the possibility of the existence of a threshold for the transport capacity for sodium by the salivary ducts of the dog's submandibular gland 10 mongrel dogs were rendered hypernatremic by the intravenous infusion of a hypertonic sodium solution. The main excretory duct was canulated and the vegetative branch of the chorda tympani nerve was maximally excited for 20-minute periods intercalated by similar periods of rest. A threshold for sodium transport was observed, dependent on the amount of sodium offered to the salivary ducts and on the salivary flow. This threshold is around 75 uEq/min with a flow of saliva of 1.3 ml per minute. The excretion of potassium in these experiments did not show a trend to reach a threshold.", "contents": "[Sodium transport and potassium excretion by the salivary ducts of the dog's submandibular gland (author's transl)]. In order to study the possibility of the existence of a threshold for the transport capacity for sodium by the salivary ducts of the dog's submandibular gland 10 mongrel dogs were rendered hypernatremic by the intravenous infusion of a hypertonic sodium solution. The main excretory duct was canulated and the vegetative branch of the chorda tympani nerve was maximally excited for 20-minute periods intercalated by similar periods of rest. A threshold for sodium transport was observed, dependent on the amount of sodium offered to the salivary ducts and on the salivary flow. This threshold is around 75 uEq/min with a flow of saliva of 1.3 ml per minute. The excretion of potassium in these experiments did not show a trend to reach a threshold."}
{"id": "PMID:461849", "title": "[Normal values of T3-index and of thyroxine (T4) (author's transl)].", "content": "The normal values of T3-index and of thyroxine (T4) have been evaluated comparing a binomial distribution to frequential curve of all the values obtained. The findings are: T3-index = 89 divided by 112; T4 = 3.7 divided by 15.3 microgram/100 cm3 of serum.", "contents": "[Normal values of T3-index and of thyroxine (T4) (author's transl)]. The normal values of T3-index and of thyroxine (T4) have been evaluated comparing a binomial distribution to frequential curve of all the values obtained. The findings are: T3-index = 89 divided by 112; T4 = 3.7 divided by 15.3 microgram/100 cm3 of serum."}
{"id": "PMID:461857", "title": "Occurence of Veillonella and Neisseria in the niches of the human oral cavity.", "content": "Samples collected from the dental surface, gingival sulcus, dorsum of the tongue and from the cheek of ten adults (18 to 32 years) were investigated to identify the Veillonella and Neisseria species present by applying the taxonomic criteria suggested in the latest edition of Bergey's Manual. The various niches contain both V. parvula and V. alcalescens, but with the predominance of strains which do not fit precisely the described catalase and putrescine requirements. Eighty-nine out of 100 isolated strains of Gram negative oxidase positive cocci could be identified as N. sicca and N. subflava; about 11 unidentified strains exhibited characteristics of N. flavescens species.", "contents": "Occurence of Veillonella and Neisseria in the niches of the human oral cavity. Samples collected from the dental surface, gingival sulcus, dorsum of the tongue and from the cheek of ten adults (18 to 32 years) were investigated to identify the Veillonella and Neisseria species present by applying the taxonomic criteria suggested in the latest edition of Bergey's Manual. The various niches contain both V. parvula and V. alcalescens, but with the predominance of strains which do not fit precisely the described catalase and putrescine requirements. Eighty-nine out of 100 isolated strains of Gram negative oxidase positive cocci could be identified as N. sicca and N. subflava; about 11 unidentified strains exhibited characteristics of N. flavescens species."}
{"id": "PMID:461858", "title": "Interactions between homologous and heterologous IgE in the induction of PCA reactions in rats.", "content": "Various inbred strains of rats presented differences in sensitivity to the induction of PCA reactions by mouse reaginic antibody. This was shown to be due to the presence of non-specific IgE in the normal sera of the bad recipients. Both positive and negative interaction, as measured by PCA reactions, could be observed between sera of the two different species. Inhibition of PCA reactions was seen when positive serum was diluted with normal serum from rat and mouse strains which were bad recipients, as opposed to what occured when sera from good recipients, were used to dilute. Synergism, on the other hand, was observed when mixtures of subsensitizing doses of both rat and mouse positive sera were tested. These results give further support to the suggestion that rat mast cells receptors for rat and mouse IgE are either the same, or are present, near enough each other, to produce steric hindrance.", "contents": "Interactions between homologous and heterologous IgE in the induction of PCA reactions in rats. Various inbred strains of rats presented differences in sensitivity to the induction of PCA reactions by mouse reaginic antibody. This was shown to be due to the presence of non-specific IgE in the normal sera of the bad recipients. Both positive and negative interaction, as measured by PCA reactions, could be observed between sera of the two different species. Inhibition of PCA reactions was seen when positive serum was diluted with normal serum from rat and mouse strains which were bad recipients, as opposed to what occured when sera from good recipients, were used to dilute. Synergism, on the other hand, was observed when mixtures of subsensitizing doses of both rat and mouse positive sera were tested. These results give further support to the suggestion that rat mast cells receptors for rat and mouse IgE are either the same, or are present, near enough each other, to produce steric hindrance."}
{"id": "PMID:461859", "title": "[Effects of parotidectomy on the blood sugar level and hepatic glycogen (author's transl].", "content": "This work was carried out in order to investigate the influence of parotid glands on the metabolism of carbohydrates in the rat. The animals were selected by age (10, 20, 30 and 40 days-old) and distributed in 3 experimental groups as follows: Group I -- Parotidectomized animals; Group II -- Sham-parotidectomized animals; and Group III -- Control animals. The animals from all the groups were sacrificed 20 days after the beginning of the experiment. From the discussion of the results it was observed that the parotidectomized animals at 20, 30 and 40 days have presented significantly larger glycaemic means than control animals. These control animals, by the other hand, have also presented significantly larger glycaemic means than sham-parotidectomized animals. Concerning the 10 days-old animals, the statistical analysis have not showed any significance among the three groups. The results obtained from hepatic glycogen dosages, in the same animals, have showed a coherence, because the animals that have presented larger glycaemic levels, have also presented less hepatic glycogen content; contrarily, the animals that have presented a less glycaemic levels, have also presented a larger amount of hepatic glycogen.", "contents": "[Effects of parotidectomy on the blood sugar level and hepatic glycogen (author's transl]. This work was carried out in order to investigate the influence of parotid glands on the metabolism of carbohydrates in the rat. The animals were selected by age (10, 20, 30 and 40 days-old) and distributed in 3 experimental groups as follows: Group I -- Parotidectomized animals; Group II -- Sham-parotidectomized animals; and Group III -- Control animals. The animals from all the groups were sacrificed 20 days after the beginning of the experiment. From the discussion of the results it was observed that the parotidectomized animals at 20, 30 and 40 days have presented significantly larger glycaemic means than control animals. These control animals, by the other hand, have also presented significantly larger glycaemic means than sham-parotidectomized animals. Concerning the 10 days-old animals, the statistical analysis have not showed any significance among the three groups. The results obtained from hepatic glycogen dosages, in the same animals, have showed a coherence, because the animals that have presented larger glycaemic levels, have also presented less hepatic glycogen content; contrarily, the animals that have presented a less glycaemic levels, have also presented a larger amount of hepatic glycogen."}
{"id": "PMID:461860", "title": "Plasma lecithin: cholesterol acyltransferase activity in normal full-term newborn infants.", "content": "Plasma lecithin: cholesterol acyltransferase (LCAT) activity was studied in 100 normal full-term newborn infants during the first few days of life. The present study shows that plasma LCAT activity present at birth-though at much lower levels than those in the maternal blood-undergoes a fall after birth followed by a rise. It is also shown that the activity of the plasma LCAT enzyme is not strictly linked to the esterification of plasma-free cholesterol in the first three days after birth. However, from the fourth day onwards, there was a good correlation between LCAT activity and esterification of plasma-free cholesterol. The results suggest that plasma LCAT in newborn infants could be partially derived from maternal sources.", "contents": "Plasma lecithin: cholesterol acyltransferase activity in normal full-term newborn infants. Plasma lecithin: cholesterol acyltransferase (LCAT) activity was studied in 100 normal full-term newborn infants during the first few days of life. The present study shows that plasma LCAT activity present at birth-though at much lower levels than those in the maternal blood-undergoes a fall after birth followed by a rise. It is also shown that the activity of the plasma LCAT enzyme is not strictly linked to the esterification of plasma-free cholesterol in the first three days after birth. However, from the fourth day onwards, there was a good correlation between LCAT activity and esterification of plasma-free cholesterol. The results suggest that plasma LCAT in newborn infants could be partially derived from maternal sources."}
{"id": "PMID:461863", "title": "[Papillosphincterotomy of Oddi's sphincter (experience in 401 cases)].", "content": "The authors present a statistics covering 401 cases in whom sphincterotomy of the Oddi sphincter was performed, in most of them for treating stenosing inflammations (papillo-odditis). Indications of this method are analized, for the surgical treatment of hydatic cysts opening in the biliary pathways, and of cholestatic hepatitis. Considerations are made on the place of sphincterotomy in the treatment of the main biliary pathway and the results are analized obtained with this method, as well as re-interventions after sphincterotomy. Some conclusions can be made on the indications and the contraindications of the method in the surgery of the hepato-choledocus.", "contents": "[Papillosphincterotomy of Oddi's sphincter (experience in 401 cases)]. The authors present a statistics covering 401 cases in whom sphincterotomy of the Oddi sphincter was performed, in most of them for treating stenosing inflammations (papillo-odditis). Indications of this method are analized, for the surgical treatment of hydatic cysts opening in the biliary pathways, and of cholestatic hepatitis. Considerations are made on the place of sphincterotomy in the treatment of the main biliary pathway and the results are analized obtained with this method, as well as re-interventions after sphincterotomy. Some conclusions can be made on the indications and the contraindications of the method in the surgery of the hepato-choledocus."}
{"id": "PMID:461865", "title": "[Surgical shock. Acute hypocatecholaminemia and therapeutic indications of an adrenocortical vasopressor sympathicolytic drug mixture. Clinical studies and experimental research].", "content": "In cases with postoperative shock and collapse a constant fall in the concentration of serum cathecholamines was noted. Thus adrenalin was reduced by 77%, noradrenalin by 86.5%, in direct proportion with the fall in the blood pressure, of the pulse amplitude and with the slowing-down (or the arrest) of the microcirculation. The reduction of serum cathecholamines also coincided with a decrease in the amount of buffer bases and of the diuresis. The adrenocortical vasopresor sympathicolithic mixture is indicated in the prolonged arterial hypotension following failure of therapy aimed at filling of the vascular bed associated with the administration of sympathicolytic drugs.", "contents": "[Surgical shock. Acute hypocatecholaminemia and therapeutic indications of an adrenocortical vasopressor sympathicolytic drug mixture. Clinical studies and experimental research]. In cases with postoperative shock and collapse a constant fall in the concentration of serum cathecholamines was noted. Thus adrenalin was reduced by 77%, noradrenalin by 86.5%, in direct proportion with the fall in the blood pressure, of the pulse amplitude and with the slowing-down (or the arrest) of the microcirculation. The reduction of serum cathecholamines also coincided with a decrease in the amount of buffer bases and of the diuresis. The adrenocortical vasopresor sympathicolithic mixture is indicated in the prolonged arterial hypotension following failure of therapy aimed at filling of the vascular bed associated with the administration of sympathicolytic drugs."}
{"id": "PMID:461866", "title": "[A surgical technic deserving reconsideration for closure of vesicovaginal fistulas: the Dittel-Forgue-Legueu operation].", "content": "A personal statistic is presented, on 24 patients with vesico-vaginal fistulae that have been operated. In 15 of the patients the Dittel-Forgue-Leguen procedure was adopted, of transperitoneo-vesical approach, and 14 good results were recorded (93%). The authors suggest that this procedure should be reconsidered, expecially for cases with very large, circular fistulae, in which the vaginal approach is not practicable, and when associated interventions are necessary, such as ureteral reimplantation, enteroplasties for enlarging the bladder capacity, resection of annexes, hysterectomy etc.", "contents": "[A surgical technic deserving reconsideration for closure of vesicovaginal fistulas: the Dittel-Forgue-Legueu operation]. A personal statistic is presented, on 24 patients with vesico-vaginal fistulae that have been operated. In 15 of the patients the Dittel-Forgue-Leguen procedure was adopted, of transperitoneo-vesical approach, and 14 good results were recorded (93%). The authors suggest that this procedure should be reconsidered, expecially for cases with very large, circular fistulae, in which the vaginal approach is not practicable, and when associated interventions are necessary, such as ureteral reimplantation, enteroplasties for enlarging the bladder capacity, resection of annexes, hysterectomy etc."}
{"id": "PMID:461864", "title": "[Venous shunts in the treatment of acute thromboses of the cavernous body].", "content": "Six cases are presented, of acute thrombosis of the cavernous bodies, that were treated surgically. In five of the cases saphenous-cavernous anastomosis was performed and in the sixth spongio-cavernous anastomosis was carried out. The results are mediocre, four of the patients remaining impotent. The cause of the failure is probably the long duration of stasis in the cavernous bodies before venous derivations were performed.", "contents": "[Venous shunts in the treatment of acute thromboses of the cavernous body]. Six cases are presented, of acute thrombosis of the cavernous bodies, that were treated surgically. In five of the cases saphenous-cavernous anastomosis was performed and in the sixth spongio-cavernous anastomosis was carried out. The results are mediocre, four of the patients remaining impotent. The cause of the failure is probably the long duration of stasis in the cavernous bodies before venous derivations were performed."}
{"id": "PMID:461869", "title": "[Unusual clinical and therapeutic aspects of intrathoracic goiter].", "content": "Out of the 220 cases of goiters operated in the clinic 19 were partially or totally intra-thoracic, as follows: --totally intra-thoracic: 7 cases; --partially intra-thoracic: 12 cases, of which: mediastino-cervical: 2 cases; cervico-mediastinal: 10 cases. Five of the totally intra-thoracic goiters were in the anterior mediastinum, while two were in the posterior mediastinum, in a retro-oesophageal position. Three of the 19 intra-thoracic goiters were malignant. The symptomatology, the diagnosis and especially the therapeutical attitude are discussed.", "contents": "[Unusual clinical and therapeutic aspects of intrathoracic goiter]. Out of the 220 cases of goiters operated in the clinic 19 were partially or totally intra-thoracic, as follows: --totally intra-thoracic: 7 cases; --partially intra-thoracic: 12 cases, of which: mediastino-cervical: 2 cases; cervico-mediastinal: 10 cases. Five of the totally intra-thoracic goiters were in the anterior mediastinum, while two were in the posterior mediastinum, in a retro-oesophageal position. Three of the 19 intra-thoracic goiters were malignant. The symptomatology, the diagnosis and especially the therapeutical attitude are discussed."}
{"id": "PMID:461870", "title": "[Surgical infections with anaerobic bacteria  (splenic abscess ruptured into the peritoneum].", "content": "A case is presented, of a patient aged 19, who, in the course of otic suppuration treated by tetracycline administration, developed a septicemic condition with a gigantic splenic abscess followed by generalized peritonitis. The initially unfavourable evolution had an improved course following splenectomy and peritoneal drainage, especially after the identification of the anaerobic germs that determined the infection (Bacteroides clostridii formis and Clostridium bifermentans) and the introduction of an adequate therapy.", "contents": "[Surgical infections with anaerobic bacteria  (splenic abscess ruptured into the peritoneum]. A case is presented, of a patient aged 19, who, in the course of otic suppuration treated by tetracycline administration, developed a septicemic condition with a gigantic splenic abscess followed by generalized peritonitis. The initially unfavourable evolution had an improved course following splenectomy and peritoneal drainage, especially after the identification of the anaerobic germs that determined the infection (Bacteroides clostridii formis and Clostridium bifermentans) and the introduction of an adequate therapy."}
{"id": "PMID:461871", "title": "[Primary splenic abscess ruptured into the peritoneal cavity (peritonitis in 3 stages)].", "content": "The authors present a case with peritonitis following rupture of a splenic abscess in a female aged 18 years. Peritonitis developed in three stages. The diagnosis before surgery was of pelvic peritonitis. Bacteriologic examination revealed the presence of B. colli. Splenectomy was followed by complete recovery of the patient. The site of the primary infection could not be determined. The authors stress the usefulness of exploration of the splenic lodge in the so-called \"primary\" generalized peritonitis, in pelvic peritonitis or in all cases when the origin of the peritoneal infection is not known.", "contents": "[Primary splenic abscess ruptured into the peritoneal cavity (peritonitis in 3 stages)]. The authors present a case with peritonitis following rupture of a splenic abscess in a female aged 18 years. Peritonitis developed in three stages. The diagnosis before surgery was of pelvic peritonitis. Bacteriologic examination revealed the presence of B. colli. Splenectomy was followed by complete recovery of the patient. The site of the primary infection could not be determined. The authors stress the usefulness of exploration of the splenic lodge in the so-called \"primary\" generalized peritonitis, in pelvic peritonitis or in all cases when the origin of the peritoneal infection is not known."}
{"id": "PMID:461873", "title": "[Influence of extracorporeal circulation on renal function].", "content": "The renal function was investigated comparatively in two lots of patients with cardiovascular diseases that had been operated with and without extra-corporeal circulation. It was shown that extra-corporeal perfusion alters the renal function in an increased number of cases (28% against 17.75%). Other parenchymatous tissues have also been involved, such as the liver and the pancreas. The humoral parameters and the clinical data are discussed, which demonstrate the organic involvement following such interventions. The good results obtained stress the necessity of an energetic treatment, which should include early dialysis.", "contents": "[Influence of extracorporeal circulation on renal function]. The renal function was investigated comparatively in two lots of patients with cardiovascular diseases that had been operated with and without extra-corporeal circulation. It was shown that extra-corporeal perfusion alters the renal function in an increased number of cases (28% against 17.75%). Other parenchymatous tissues have also been involved, such as the liver and the pancreas. The humoral parameters and the clinical data are discussed, which demonstrate the organic involvement following such interventions. The good results obtained stress the necessity of an energetic treatment, which should include early dialysis."}
{"id": "PMID:461874", "title": "[Role of the adrenal medulla in reaction of the body to stress (clinical and laboratory studies and experimental research)].", "content": "The authors have carried out a clinical study of acute medico-surgical complications occuring in patients hospitalized following psychical stress, myocardial infarction, operatory shock and after the earthquake. In patients with duodenal ulcers and hyperacidity due to vagal neurogenic origins it was noted the presence of a sympatico-adrenergic constitutional background in 38% of the cases. By applying chemical sclerosis of the adrenals medullary the role of the medullary was demonstrated in the production of acute digestive lesions due to stress, as a result of standard electrical stimulus and of their influence on the bioelectrical reactivity of the brain, on the active learning behaviour by conditioned reaction and on the number of the circulating eosinophils following injections of A.C.T.H.", "contents": "[Role of the adrenal medulla in reaction of the body to stress (clinical and laboratory studies and experimental research)]. The authors have carried out a clinical study of acute medico-surgical complications occuring in patients hospitalized following psychical stress, myocardial infarction, operatory shock and after the earthquake. In patients with duodenal ulcers and hyperacidity due to vagal neurogenic origins it was noted the presence of a sympatico-adrenergic constitutional background in 38% of the cases. By applying chemical sclerosis of the adrenals medullary the role of the medullary was demonstrated in the production of acute digestive lesions due to stress, as a result of standard electrical stimulus and of their influence on the bioelectrical reactivity of the brain, on the active learning behaviour by conditioned reaction and on the number of the circulating eosinophils following injections of A.C.T.H."}
{"id": "PMID:461868", "title": "[Anatomoclinical and therapeutic considerations on non-lithiasic benign papillo-oddian stenosis].", "content": "Based on their personal experience involving 19 cases of benign, non-lithiasic stenoses of the Oddi sphincter (in a total of 2,500 interventions on the cholecyst and the choledocus), the authors review the etiopathogenic, diagnostic and therapeutic aspects. The difficulty is stressed, encountered in evaluating the reversibility of these lesions and the codification of the therapeutic attitudes.", "contents": "[Anatomoclinical and therapeutic considerations on non-lithiasic benign papillo-oddian stenosis]. Based on their personal experience involving 19 cases of benign, non-lithiasic stenoses of the Oddi sphincter (in a total of 2,500 interventions on the cholecyst and the choledocus), the authors review the etiopathogenic, diagnostic and therapeutic aspects. The difficulty is stressed, encountered in evaluating the reversibility of these lesions and the codification of the therapeutic attitudes."}
{"id": "PMID:461875", "title": "[Pancreatic fistulas].", "content": "Based on 17 observations the authors review the major etiopathogenic, evolutive, clinical and therapeutic aspects of pancreatic fistulae. In 14 cases the cure was achieved by medical treatment while surgery was resorted to in only three cases. The medical measures are discussed, as well as the indications for surgical treatment.", "contents": "[Pancreatic fistulas]. Based on 17 observations the authors review the major etiopathogenic, evolutive, clinical and therapeutic aspects of pancreatic fistulae. In 14 cases the cure was achieved by medical treatment while surgery was resorted to in only three cases. The medical measures are discussed, as well as the indications for surgical treatment."}
{"id": "PMID:461876", "title": "[Splenectomy in chronic hepatitis with an autoimmune component].", "content": "The authors present a synthesis of studies performed on the role of the spleen in chronic active-digestive hepatitis with auto-immune participation. The study was based on a group of 48 patients out of which a lot of 20 patients was selected, with histological diagnosis of chronic active and aggressive hepatitis, in whom the presence of AgAu was ascertained, as well as of antiliver antibodies. From the immunological viewpoint, the effect of splenectomy was manifested, by a rapid and significant decrease of anti-liver antibodies mainly produced by the spleen, immediately following the intervention. In most of the patients the IgM antibodies disappeared somewhat later. At the same time the chronic hepatitis lesions were stabilized, the biological signs were improved and the clinical evolution of the patients was good. The authors consider that splenectomy in this category of hepatopathies is an immuno-suppressive therapy that may arrest the evolution towards cirrhosis.", "contents": "[Splenectomy in chronic hepatitis with an autoimmune component]. The authors present a synthesis of studies performed on the role of the spleen in chronic active-digestive hepatitis with auto-immune participation. The study was based on a group of 48 patients out of which a lot of 20 patients was selected, with histological diagnosis of chronic active and aggressive hepatitis, in whom the presence of AgAu was ascertained, as well as of antiliver antibodies. From the immunological viewpoint, the effect of splenectomy was manifested, by a rapid and significant decrease of anti-liver antibodies mainly produced by the spleen, immediately following the intervention. In most of the patients the IgM antibodies disappeared somewhat later. At the same time the chronic hepatitis lesions were stabilized, the biological signs were improved and the clinical evolution of the patients was good. The authors consider that splenectomy in this category of hepatopathies is an immuno-suppressive therapy that may arrest the evolution towards cirrhosis."}
{"id": "PMID:461903", "title": "[Bioelectric behaviour of intestinal wall in the earthworm Scherotheca sp (author's transl)].", "content": "Transmural potential difference (TPD) and short circuit current in the Scherotheca sp. intestinal wall in in vitro preparations have been studied, as well as their variations as related to ionic substitutions. Sodium ion seems to participate the most in the genesis of registered potential, so that its total substitution in the medium brings about the disappearance of the potential. Transmural potential relies on metabolic energy and is inhibited by N2, 2,4-DNP and CNK. Ouabain inhibits TPD only when present on the serosal side. This might indicate the existence on that side of a Na+ pump responsible for the active transport of the cation from mucosal.", "contents": "[Bioelectric behaviour of intestinal wall in the earthworm Scherotheca sp (author's transl)]. Transmural potential difference (TPD) and short circuit current in the Scherotheca sp. intestinal wall in in vitro preparations have been studied, as well as their variations as related to ionic substitutions. Sodium ion seems to participate the most in the genesis of registered potential, so that its total substitution in the medium brings about the disappearance of the potential. Transmural potential relies on metabolic energy and is inhibited by N2, 2,4-DNP and CNK. Ouabain inhibits TPD only when present on the serosal side. This might indicate the existence on that side of a Na+ pump responsible for the active transport of the cation from mucosal."}
{"id": "PMID:461904", "title": "Studies on the competitive intestinal absorption of amino acids, and sugars in two teleost fishes.", "content": "In both fishes Ophiocephalus punctatus and Heteropneustes fossilis, the compound of the same group inhibited the transport rate of another compound such as, both glucose and fructose inhibited the uptake of xylose. Glucose was comparatively more inhibitory in nature than fructose. Transport of one amino acid was also inhibited in the presence of another amino acid. In glycine transport, leucine was stronger inhibitor than tyrosine. Almost similar results have been observed in both fishes irrespective of different feeding habits.", "contents": "Studies on the competitive intestinal absorption of amino acids, and sugars in two teleost fishes. In both fishes Ophiocephalus punctatus and Heteropneustes fossilis, the compound of the same group inhibited the transport rate of another compound such as, both glucose and fructose inhibited the uptake of xylose. Glucose was comparatively more inhibitory in nature than fructose. Transport of one amino acid was also inhibited in the presence of another amino acid. In glycine transport, leucine was stronger inhibitor than tyrosine. Almost similar results have been observed in both fishes irrespective of different feeding habits."}
{"id": "PMID:461905", "title": "Amino acids and lactate efflux from in vitro incubated soleus muscles of suckling rats.", "content": "The net in vitro release of lactate, pyruvate, alanine, glutamate, glutamine and total amino acids from soleus muscles of suckling rats was studied. In all cases, the net output of metabolites was considerably higher in young animals than in 30 day old controls. Glutamine efflux of 1 day old pups was very low, probably due to lack of effectivity of the glutamine synthesizing system. The considerable output of amino acids in the younger animals was partly due to depletion of internal amino acid pools.", "contents": "Amino acids and lactate efflux from in vitro incubated soleus muscles of suckling rats. The net in vitro release of lactate, pyruvate, alanine, glutamate, glutamine and total amino acids from soleus muscles of suckling rats was studied. In all cases, the net output of metabolites was considerably higher in young animals than in 30 day old controls. Glutamine efflux of 1 day old pups was very low, probably due to lack of effectivity of the glutamine synthesizing system. The considerable output of amino acids in the younger animals was partly due to depletion of internal amino acid pools."}
{"id": "PMID:461906", "title": "Standardization and clinical application of a radioimmunoassay for human calcitonin.", "content": "A radioimmunoassay for the measurement of Human Calcitonin (HCT) is described. Serum levels of HCT in normal subjects and in individuals under different pathological conditions have been studied with this method. HCT labelling is performed following the chloramine T method of Hunter and Greenwood. Adding successively Quso G-32 (a finely powdered silica) and an anion exchange resin (AGI-X10 resin), to the tracer, reduces both the damaged fraction and the free isotope which originate during storage. Purification of the labelled hormone is carried out through a Sephadex G-50 gel column. Sera stored at --20 degrees C preserves its immunoreactivity up to 4 months after extraction. The mean basal HCT levels in 110 fasting normal persons is 277 +/- 123 pg/ml (undetectable 8.28%). No significant correlation between HCT levels and various serum ions has been observed. Basal HCT values in seven patients with medullary thyroid carcinoma (MTC), oscillated between 5 and 110 ng/ml, while in six other patients with non medullary thyroid carcinoma the values remained within normal range. Both a calcium infustion and a pentagastrin injection are used to stimulate HCT secretion. The increase of HCT basal levels produced by the latter in normal controls and in patients with MTC, is faster and more intense. Calcium infusion produced a significant correlation between calcium and the increased HCT levels only in patients affected with MTC.", "contents": "Standardization and clinical application of a radioimmunoassay for human calcitonin. A radioimmunoassay for the measurement of Human Calcitonin (HCT) is described. Serum levels of HCT in normal subjects and in individuals under different pathological conditions have been studied with this method. HCT labelling is performed following the chloramine T method of Hunter and Greenwood. Adding successively Quso G-32 (a finely powdered silica) and an anion exchange resin (AGI-X10 resin), to the tracer, reduces both the damaged fraction and the free isotope which originate during storage. Purification of the labelled hormone is carried out through a Sephadex G-50 gel column. Sera stored at --20 degrees C preserves its immunoreactivity up to 4 months after extraction. The mean basal HCT levels in 110 fasting normal persons is 277 +/- 123 pg/ml (undetectable 8.28%). No significant correlation between HCT levels and various serum ions has been observed. Basal HCT values in seven patients with medullary thyroid carcinoma (MTC), oscillated between 5 and 110 ng/ml, while in six other patients with non medullary thyroid carcinoma the values remained within normal range. Both a calcium infustion and a pentagastrin injection are used to stimulate HCT secretion. The increase of HCT basal levels produced by the latter in normal controls and in patients with MTC, is faster and more intense. Calcium infusion produced a significant correlation between calcium and the increased HCT levels only in patients affected with MTC."}
{"id": "PMID:461907", "title": "Alterations induced by large doses of furosemide in chronic renal insufficiency.", "content": "Eleven patients with different degrees of renal failure with creatinine clearances between 7 and 32 ml/min have been studied. After a standard water overload and control periods of clearances, furosemide 1 g was given/i.v. There followed significant increase of renal plasma flow and glomerular filtration rate. In one case the increase was maintained during a follow up period of 3 hours. A significant increase was evident in phosphate, uric acid, sodium, potassium, and calcium clearances, as well as an increase in the sodium delivery to the distal nephron and a decrease in tubular reabsorption of phosphate. All this may be interpreted as the result of renal vasodilation induced by furosemide and its effect upon the proximal tubule and on Henle's loop.", "contents": "Alterations induced by large doses of furosemide in chronic renal insufficiency. Eleven patients with different degrees of renal failure with creatinine clearances between 7 and 32 ml/min have been studied. After a standard water overload and control periods of clearances, furosemide 1 g was given/i.v. There followed significant increase of renal plasma flow and glomerular filtration rate. In one case the increase was maintained during a follow up period of 3 hours. A significant increase was evident in phosphate, uric acid, sodium, potassium, and calcium clearances, as well as an increase in the sodium delivery to the distal nephron and a decrease in tubular reabsorption of phosphate. All this may be interpreted as the result of renal vasodilation induced by furosemide and its effect upon the proximal tubule and on Henle's loop."}
{"id": "PMID:461908", "title": "Enzymatic disappearance of hydroxylamine in the presence of GABA.", "content": "This paper presents data on the elimination of hydroxylamine from Lupinus albus seeds when they were germinated in the presence of GABA and hydroxylamine. The possibility of an enzymatic reaction. ATP dependent, between GABA and hydroxylamine is discussed. Some kinetic properties from this reaction are studied.", "contents": "Enzymatic disappearance of hydroxylamine in the presence of GABA. This paper presents data on the elimination of hydroxylamine from Lupinus albus seeds when they were germinated in the presence of GABA and hydroxylamine. The possibility of an enzymatic reaction. ATP dependent, between GABA and hydroxylamine is discussed. Some kinetic properties from this reaction are studied."}
{"id": "PMID:461909", "title": "[Direct method for osmotic pressure measurement (author's transl)].", "content": "A new dynamic method to measure osmotic pressure was presented. The method is easy and with only a 1.8% degree of error. All determinations were done with an osmometer, equipped with three small chambers of methyl metacrilate and dialyser membranes. The central area had two capillaries: one for measuring and the other for changing dynamic pressure. The data from different albumin concentrations were satisfactory, and they agreed with published results from others. The present results indicate that osmotic pressure is an exponential function of albumin concentration.", "contents": "[Direct method for osmotic pressure measurement (author's transl)]. A new dynamic method to measure osmotic pressure was presented. The method is easy and with only a 1.8% degree of error. All determinations were done with an osmometer, equipped with three small chambers of methyl metacrilate and dialyser membranes. The central area had two capillaries: one for measuring and the other for changing dynamic pressure. The data from different albumin concentrations were satisfactory, and they agreed with published results from others. The present results indicate that osmotic pressure is an exponential function of albumin concentration."}
{"id": "PMID:461910", "title": "[Conditions for greater survival of neonatally thymectomized Swiss mice (author's transl)].", "content": "A total of 385 albino Swiss mice, of both sexes, between 24 and 30 hours of age are neonatally thymectomized, by means of a suction pump method. One batch is operated under ether anesthesia, another with sodium pentobarbital, and a third one with Cryoanesthesia. The last batch is subdivided into 2. In one of these the mother is anesthetized with sodium pentobarbital intraperitoneally, during the postoperative recovery of the litter, and in the other the mother is left without treatment. The mothers of the first two batches of thymectomized animals are anesthetized with the same products as their offspring. The percentage of survival of thymectomized breeding mice in greatest in the batch operated under Cryoanesthesia and whose mothers received the above mentioned treatment. Statistically the differences are very significant and they might be accounted to the fact that the mothers are anesthetized while their offspring normalize their physiological constants with better recovery in feeding, hydration, shelter and rest.", "contents": "[Conditions for greater survival of neonatally thymectomized Swiss mice (author's transl)]. A total of 385 albino Swiss mice, of both sexes, between 24 and 30 hours of age are neonatally thymectomized, by means of a suction pump method. One batch is operated under ether anesthesia, another with sodium pentobarbital, and a third one with Cryoanesthesia. The last batch is subdivided into 2. In one of these the mother is anesthetized with sodium pentobarbital intraperitoneally, during the postoperative recovery of the litter, and in the other the mother is left without treatment. The mothers of the first two batches of thymectomized animals are anesthetized with the same products as their offspring. The percentage of survival of thymectomized breeding mice in greatest in the batch operated under Cryoanesthesia and whose mothers received the above mentioned treatment. Statistically the differences are very significant and they might be accounted to the fact that the mothers are anesthetized while their offspring normalize their physiological constants with better recovery in feeding, hydration, shelter and rest."}
{"id": "PMID:461911", "title": "[The effect of the follicle-stimulating hormone on testosterone liberation induced by lh in the isolated leydig cell (author's transl)].", "content": "The effect of FSH on the liberation of testosterone induced by LH in the Leydig cell has been investigated. Leydig cells were obtained from adult male rats, and were incubated with LH in one group and with LH and FSH in another group. Incubation was kept at 37 degrees C, 120 cycles/min, in oxygen-carbon dioxide (95:5) atmosphere. Liberated testosterone was measured by RIA, at different periods of incubation. Other group of cells, in identical experimental conditions, were incubated for 120 min with LH and FSH, where the FSH had been previously incubated with anti-FSH antibody. The cells incubated with LH and FSH liberated more testosterone than those incubated with LH. However this effect disappeared when the added FSH had been previously incubated with its antibody. The results show clearly that FSH liberates testosterone by the action of LH, which must be specific since it disappears when the hormone is incubated with its antibody.", "contents": "[The effect of the follicle-stimulating hormone on testosterone liberation induced by lh in the isolated leydig cell (author's transl)]. The effect of FSH on the liberation of testosterone induced by LH in the Leydig cell has been investigated. Leydig cells were obtained from adult male rats, and were incubated with LH in one group and with LH and FSH in another group. Incubation was kept at 37 degrees C, 120 cycles/min, in oxygen-carbon dioxide (95:5) atmosphere. Liberated testosterone was measured by RIA, at different periods of incubation. Other group of cells, in identical experimental conditions, were incubated for 120 min with LH and FSH, where the FSH had been previously incubated with anti-FSH antibody. The cells incubated with LH and FSH liberated more testosterone than those incubated with LH. However this effect disappeared when the added FSH had been previously incubated with its antibody. The results show clearly that FSH liberates testosterone by the action of LH, which must be specific since it disappears when the hormone is incubated with its antibody."}
{"id": "PMID:461912", "title": "Method for successive absorptions with intestinal perfusion in vivo.", "content": "A simple technique to study intestinal absorption in vivo, a modification of that of SOLS and PONZ (34), is described. It uses a perfusion pump, with or without recycling of the solution along the intestinal lumen, and allows the carrying out of a series of successive absorption periods with high comparative value on the same intestinal segment. The technique is applied to sugar absorption in rat and hamster, but it can be extended to other substrates and other animal species. For each concentration of sugar, the intestinal absorption rate of glucose remains constant along a number of 1 min successive periods.", "contents": "Method for successive absorptions with intestinal perfusion in vivo. A simple technique to study intestinal absorption in vivo, a modification of that of SOLS and PONZ (34), is described. It uses a perfusion pump, with or without recycling of the solution along the intestinal lumen, and allows the carrying out of a series of successive absorption periods with high comparative value on the same intestinal segment. The technique is applied to sugar absorption in rat and hamster, but it can be extended to other substrates and other animal species. For each concentration of sugar, the intestinal absorption rate of glucose remains constant along a number of 1 min successive periods."}
{"id": "PMID:461941", "title": "[Rehabilitation of brain-injured patients--demonstrated with the help of a successfully performed individual rehabilitation plan (author's transl)].", "content": "According to 5 Sect. 3 of the Act on the Standardisation of Rehabilitation Services (Rehabilitations-Angleichungsgesetz) an individual rehabilitation plan must be set up if several rehabilitation measures, e.g., medical, vocational and social measures are necessary, or, if different rehabilitation agencies are financially responsible for these procedures. This applies, as a rule, to the rehabilitation efforts for the severely brain-injured. The case history of a young brain-injured patient is used to demonstrate that an overal improvement can be achieved if a close co-operation between the hospital, rehabilitation centre, attending doctor, rehabilitation agencies and employers is ensured. The different measures must be adapted to the individual degree of work tolerance of the patient. Out-patient measures should be carried out -- and this not only because of the lower costs involved. The so-called \"Anschlussheilverfahren\" which means a treatment programme following the acute phase, initiated by the responsible agencies for the pension insurance scheme, does not seen to be the appropriate routine treatment to be carried out in the rehabilitation of the brain-injured, as they require more comprehensive and long-term therapy.", "contents": "[Rehabilitation of brain-injured patients--demonstrated with the help of a successfully performed individual rehabilitation plan (author's transl)]. According to 5 Sect. 3 of the Act on the Standardisation of Rehabilitation Services (Rehabilitations-Angleichungsgesetz) an individual rehabilitation plan must be set up if several rehabilitation measures, e.g., medical, vocational and social measures are necessary, or, if different rehabilitation agencies are financially responsible for these procedures. This applies, as a rule, to the rehabilitation efforts for the severely brain-injured. The case history of a young brain-injured patient is used to demonstrate that an overal improvement can be achieved if a close co-operation between the hospital, rehabilitation centre, attending doctor, rehabilitation agencies and employers is ensured. The different measures must be adapted to the individual degree of work tolerance of the patient. Out-patient measures should be carried out -- and this not only because of the lower costs involved. The so-called \"Anschlussheilverfahren\" which means a treatment programme following the acute phase, initiated by the responsible agencies for the pension insurance scheme, does not seen to be the appropriate routine treatment to be carried out in the rehabilitation of the brain-injured, as they require more comprehensive and long-term therapy."}
{"id": "PMID:461942", "title": "[Motor goals of therapeutic horseback riding for cerebral palsied children (author's transl)].", "content": "The article reviews in a critical survey the current opinions on the possibilities of improving the motor functions of cerebral palsied children with the help of riding therapy. Furthermore, the essential motor difficulties with spasm, athetosis and ataxia are described. It is demonstrated that only a small number of these typical difficulties can be tackled by means of riding therapy and that some key problems, particularly encountered by the spastics, cannot be solved. If, despite these facts, the favourable effects of riding on the cerebral palsied cannot be denied, then this must be attributed, first of all, to the unique psychological motivation derived from riding. Credit is given to the effects on the autonomic nervous system, the psyche, the world of experience and the behaviour.", "contents": "[Motor goals of therapeutic horseback riding for cerebral palsied children (author's transl)]. The article reviews in a critical survey the current opinions on the possibilities of improving the motor functions of cerebral palsied children with the help of riding therapy. Furthermore, the essential motor difficulties with spasm, athetosis and ataxia are described. It is demonstrated that only a small number of these typical difficulties can be tackled by means of riding therapy and that some key problems, particularly encountered by the spastics, cannot be solved. If, despite these facts, the favourable effects of riding on the cerebral palsied cannot be denied, then this must be attributed, first of all, to the unique psychological motivation derived from riding. Credit is given to the effects on the autonomic nervous system, the psyche, the world of experience and the behaviour."}
{"id": "PMID:461943", "title": "[Judo -- a contribution to rehabilitation (author's transl)].", "content": "A judo group was formed at the Wolfsburg sports club with twenty 9 to 15 years old children who received treatment at the centre for developmental diagnostics and pediatrics. The children suffered primarily from more or less pronounced cerebral motor disturbances. Other functional disturbances such as perceptual disorders, language and reactive behavioural disturbances completed the picture of minimal brain-damage. The training programme attributed more importance to functional judo exercises than to perfect judo techniques. Allowances were made for individual difficulties. After 15 months of training improvements were observed in the motor, psychological and perceptual fields. This is why the authors believe it is worthwile to consider judo also in connection with rehabilitation.", "contents": "[Judo -- a contribution to rehabilitation (author's transl)]. A judo group was formed at the Wolfsburg sports club with twenty 9 to 15 years old children who received treatment at the centre for developmental diagnostics and pediatrics. The children suffered primarily from more or less pronounced cerebral motor disturbances. Other functional disturbances such as perceptual disorders, language and reactive behavioural disturbances completed the picture of minimal brain-damage. The training programme attributed more importance to functional judo exercises than to perfect judo techniques. Allowances were made for individual difficulties. After 15 months of training improvements were observed in the motor, psychological and perceptual fields. This is why the authors believe it is worthwile to consider judo also in connection with rehabilitation."}
{"id": "PMID:461944", "title": "[Community psychological principles as the basis for the setting up and the organisation of a psycho-social counselling service at a vocational re-training centre (author's transl)].", "content": "In community psychology the individuum-centred medical model of disease, which, as a rule, determines the psychological and psychotherapeutic work has superseded socio-psychological concepts and approaches. The authors describe the basic requirements for a psychosocial counselling service at a vocational retraining centre which is guided by community psychological ideas and principles. The article reviews the manifold problems of the rehabilitees, the comprehensive services and the efficiency of the intervention strategies recorded over a two-year period. Furthermore, the authors discuss their experiences with preventive measures in connection with both early detection and prevention of problems. Through its adjustment to the concept of community psychology the responsibilities of psychological and psychotherapeutic services within an institution obtain a considerably higher differentiated structure, and at the same time, meet the demands more adequatedly.", "contents": "[Community psychological principles as the basis for the setting up and the organisation of a psycho-social counselling service at a vocational re-training centre (author's transl)]. In community psychology the individuum-centred medical model of disease, which, as a rule, determines the psychological and psychotherapeutic work has superseded socio-psychological concepts and approaches. The authors describe the basic requirements for a psychosocial counselling service at a vocational retraining centre which is guided by community psychological ideas and principles. The article reviews the manifold problems of the rehabilitees, the comprehensive services and the efficiency of the intervention strategies recorded over a two-year period. Furthermore, the authors discuss their experiences with preventive measures in connection with both early detection and prevention of problems. Through its adjustment to the concept of community psychology the responsibilities of psychological and psychotherapeutic services within an institution obtain a considerably higher differentiated structure, and at the same time, meet the demands more adequatedly."}
{"id": "PMID:461945", "title": "[Emancipatory rehabilitation in logopedics (author's transl)].", "content": "The authors tries to answer the question whether logopedical rehabilitation in its present form meets the requirements of its object, i.e., the speech impaired individual as seen from the perspective of prevention or reduction of his social and vocational disadvantages resulting from the language disorder. Against the background of society's \"deficiency -- classification-attitude\" towards the speech impaired, logopedical rehabilitation should enhance more intensively the emancipation of the speech deviant individual. The study of the practical realization of this requirement demonstrates that logopedics -- still considerably influenced by medical conceptual models -- which is understandable both historically and socially, has not made steps towards emancipatory rehabilitation. Prospects and approaches towards emancipatory rehabilitation resulting in an improvement of the speech disorder are demonstrated.", "contents": "[Emancipatory rehabilitation in logopedics (author's transl)]. The authors tries to answer the question whether logopedical rehabilitation in its present form meets the requirements of its object, i.e., the speech impaired individual as seen from the perspective of prevention or reduction of his social and vocational disadvantages resulting from the language disorder. Against the background of society's \"deficiency -- classification-attitude\" towards the speech impaired, logopedical rehabilitation should enhance more intensively the emancipation of the speech deviant individual. The study of the practical realization of this requirement demonstrates that logopedics -- still considerably influenced by medical conceptual models -- which is understandable both historically and socially, has not made steps towards emancipatory rehabilitation. Prospects and approaches towards emancipatory rehabilitation resulting in an improvement of the speech disorder are demonstrated."}
{"id": "PMID:461946", "title": "[Emancipatory rehabilitation in logopedics -- comments from the medico-rehabilitative viewpoint (author's transl)].", "content": "In the Federal Republic of Germany speech disturbances, as sub-group of communication disorders, are treated by two professional groups: a) free practising logopedists who chose their team themselves as well as logopedists who are working in a clinic team and b) \"Sprachheilp\u00e4dagogen\" (speech therapists) working at special education facilities. Irrespective of the age of the patient, treatment by logopedists is paid by the health insurance funds; special education therapy is paid by the financially responsible school agencies. The basis of the disease is not a deviation but a disturbance of the central nervous system which can be determined by medical, phoniatric-neurological diagnostic means.", "contents": "[Emancipatory rehabilitation in logopedics -- comments from the medico-rehabilitative viewpoint (author's transl)]. In the Federal Republic of Germany speech disturbances, as sub-group of communication disorders, are treated by two professional groups: a) free practising logopedists who chose their team themselves as well as logopedists who are working in a clinic team and b) \"Sprachheilp\u00e4dagogen\" (speech therapists) working at special education facilities. Irrespective of the age of the patient, treatment by logopedists is paid by the health insurance funds; special education therapy is paid by the financially responsible school agencies. The basis of the disease is not a deviation but a disturbance of the central nervous system which can be determined by medical, phoniatric-neurological diagnostic means."}
{"id": "PMID:461948", "title": "Relaxant action of histamine on rabbit trachea: possible existence of third histamine receptor subtype.", "content": "The carbachol contracted rabbit trachea relaxes to isoproterenol, PGE1, E2, histamine and bradykinin. Histamine-induced relaxations were resistant to mepyramine (an H1-antagonist), burimamide, cimetidine, metiamide (H2-antagonists), propranolol (a beta-adrenoceptor blocker), indomethacin (a potent inhibitor of prostaglandin synthesis), trasylol (an inhibitor of kallikreins), aminophylline (a purinergic receptor blocker) and dibenzyline (a \"D\"-tryptamine receptor antagonist). The evidence achieved with these pharmacological agents appears to suggest that the chemical mediator responsible for the relaxant response to histamine is unlikely to be: catecholamines, prostaglandins, 5-HT, adenosine or ATP or kinins. The existence of H2-(iso or H3) histamine receptor subtype in rabbit trachea is postulated.", "contents": "Relaxant action of histamine on rabbit trachea: possible existence of third histamine receptor subtype. The carbachol contracted rabbit trachea relaxes to isoproterenol, PGE1, E2, histamine and bradykinin. Histamine-induced relaxations were resistant to mepyramine (an H1-antagonist), burimamide, cimetidine, metiamide (H2-antagonists), propranolol (a beta-adrenoceptor blocker), indomethacin (a potent inhibitor of prostaglandin synthesis), trasylol (an inhibitor of kallikreins), aminophylline (a purinergic receptor blocker) and dibenzyline (a \"D\"-tryptamine receptor antagonist). The evidence achieved with these pharmacological agents appears to suggest that the chemical mediator responsible for the relaxant response to histamine is unlikely to be: catecholamines, prostaglandins, 5-HT, adenosine or ATP or kinins. The existence of H2-(iso or H3) histamine receptor subtype in rabbit trachea is postulated."}
{"id": "PMID:461949", "title": "Pharmacological characterization of the rabbit lung strip.", "content": "Isolated rabbit lung parenchymal strips contracted to epinephrine (E), norepinephrine (NE), phenylephrine (PE), histamine, carbachol, PGF2alpha, bradykinin and 5-HT. Lung strips which were maximally contracted to histamine, carbachol and PGF2alpha, relaxed to isoproterenol, salbutamol, terbutaline and PGE1 however, they exhibited superimposed contractions to E, NE and PE. Atropine and mepyramine selectively antagonized contractions to carbachol and histamine respectively, without modifying contractions to the sympathomimetic agents. Phenotolamine antagonized responses to E, NE and PE. The results of this investigation suggest the presence of alpha- and beta-adrenergic and H1-histamine receptors in the rabbit lung strip.", "contents": "Pharmacological characterization of the rabbit lung strip. Isolated rabbit lung parenchymal strips contracted to epinephrine (E), norepinephrine (NE), phenylephrine (PE), histamine, carbachol, PGF2alpha, bradykinin and 5-HT. Lung strips which were maximally contracted to histamine, carbachol and PGF2alpha, relaxed to isoproterenol, salbutamol, terbutaline and PGE1 however, they exhibited superimposed contractions to E, NE and PE. Atropine and mepyramine selectively antagonized contractions to carbachol and histamine respectively, without modifying contractions to the sympathomimetic agents. Phenotolamine antagonized responses to E, NE and PE. The results of this investigation suggest the presence of alpha- and beta-adrenergic and H1-histamine receptors in the rabbit lung strip."}
{"id": "PMID:461950", "title": "The interaction of enkephalins with substance P on vascular smooth muscle.", "content": "Interactions between SP and the enkephalins have bben reported in the CNS, and the possibility of a more peripheral site of interaction has now been investigated. SP (10(-6) - 10(-4) g/ml) induced dose-related contractions of the rabbit isolated aorta, which were resistant to alpha-adrenoceptor blockade (phentolamine, 5 x 10(-6) g/ml). Neither met- nor leu-enkephalin had any intrinsic action on the aorta, but met-enkephalin at 5 x 10(-6) g/ml significantly inhibited responses to SP but not to NE. Morphine (5 x 10(-6) g/ml) potentiated responses to NE, but had no significant effect on responses to SP. It is apparent that SP and met-enkephalin act on a common receptor on vascular smooth muscle, that met-enkephalin has no intrinsic action on this receptor, and that the receptor is morphine-insensitive.", "contents": "The interaction of enkephalins with substance P on vascular smooth muscle. Interactions between SP and the enkephalins have bben reported in the CNS, and the possibility of a more peripheral site of interaction has now been investigated. SP (10(-6) - 10(-4) g/ml) induced dose-related contractions of the rabbit isolated aorta, which were resistant to alpha-adrenoceptor blockade (phentolamine, 5 x 10(-6) g/ml). Neither met- nor leu-enkephalin had any intrinsic action on the aorta, but met-enkephalin at 5 x 10(-6) g/ml significantly inhibited responses to SP but not to NE. Morphine (5 x 10(-6) g/ml) potentiated responses to NE, but had no significant effect on responses to SP. It is apparent that SP and met-enkephalin act on a common receptor on vascular smooth muscle, that met-enkephalin has no intrinsic action on this receptor, and that the receptor is morphine-insensitive."}
{"id": "PMID:461951", "title": "Phenotyping of South African black tuberculosis patients for inactivation of isoniazid.", "content": "Studies of isoniazid inactivation rates in black tuberculosis patients from South Africa and Birmingham, Alabama, showed a higher frequency of fast inactivation than those of North American Caucasian patients. After an oral test dose of 10 mg/kg isoniazid the percentage of fast inactivators in black patients of South Africa and Alabama was close to 60% while in North American white participants (Canadian and Birmingham Caucasians), the frequency of fast inactivation was approximately 40%.", "contents": "Phenotyping of South African black tuberculosis patients for inactivation of isoniazid. Studies of isoniazid inactivation rates in black tuberculosis patients from South Africa and Birmingham, Alabama, showed a higher frequency of fast inactivation than those of North American Caucasian patients. After an oral test dose of 10 mg/kg isoniazid the percentage of fast inactivators in black patients of South Africa and Alabama was close to 60% while in North American white participants (Canadian and Birmingham Caucasians), the frequency of fast inactivation was approximately 40%."}
{"id": "PMID:461952", "title": "Plasma nicotine pharmacokinetics in dogs after intravenous administration: determination by radioimmunoassay.", "content": "Nicotine plasma levels in dogs were examined by radioimmunoassay after intravenous administration of S-nicotine at the dosage of 100 microgram/kg. After a rapid decline of nicotine level in plasma immediately following the injection, retention of the level was observed until 30 min. From that point the plasma level decreased monoexponentially. A biological half life (t 1/2, beta) of 0.61 hr we observed in dogs was similar to that in man and one half of that in rats. Total body clearance (TBC) and apparent volume of distribution (Vd, beta) in dogs were about 1/5 of that in rats. The results suggested that nicotine does not distribute in dogs as readily as in rats. Similarity in a biological half life of nicotine in dogs and humans suggested that the dog may be a better experimental model than the rate in the investigation of pharmacokinetic parameters for nicotine.", "contents": "Plasma nicotine pharmacokinetics in dogs after intravenous administration: determination by radioimmunoassay. Nicotine plasma levels in dogs were examined by radioimmunoassay after intravenous administration of S-nicotine at the dosage of 100 microgram/kg. After a rapid decline of nicotine level in plasma immediately following the injection, retention of the level was observed until 30 min. From that point the plasma level decreased monoexponentially. A biological half life (t 1/2, beta) of 0.61 hr we observed in dogs was similar to that in man and one half of that in rats. Total body clearance (TBC) and apparent volume of distribution (Vd, beta) in dogs were about 1/5 of that in rats. The results suggested that nicotine does not distribute in dogs as readily as in rats. Similarity in a biological half life of nicotine in dogs and humans suggested that the dog may be a better experimental model than the rate in the investigation of pharmacokinetic parameters for nicotine."}
{"id": "PMID:461953", "title": "Measurement of naphthoxylactic and naphtoxyacetic acid in human plasma following propranolol administration.", "content": "A method for the quantitative determination of naphthoxylactic acid and naphthoxyacetic acid, two acidic metabolites of propranolol, in human plasma is described. Preliminary data from hypertensive patients receiving chronic propranolol therapy and from normal volunteers who took a single 80 mg oral propranolol dose revealed that naphthoxylactic acid plasma levels were 4 to 20 times that of propranolol whereas naphthoxyacetic acid levels were equivalent to or less than the plasma concentrations of propranolol.", "contents": "Measurement of naphthoxylactic and naphtoxyacetic acid in human plasma following propranolol administration. A method for the quantitative determination of naphthoxylactic acid and naphthoxyacetic acid, two acidic metabolites of propranolol, in human plasma is described. Preliminary data from hypertensive patients receiving chronic propranolol therapy and from normal volunteers who took a single 80 mg oral propranolol dose revealed that naphthoxylactic acid plasma levels were 4 to 20 times that of propranolol whereas naphthoxyacetic acid levels were equivalent to or less than the plasma concentrations of propranolol."}
{"id": "PMID:461954", "title": "On the regulation of antipyrine and oxazepam metabolism in man.", "content": "The metabolism of antipyrine and oxazepam has been measured in twelve healthy subjects. Antipyrine, an analgesic and antipyretic drug, is extensively metabolized by oxidation at various sites and oxazepam, a benzodiazepine, is almost exclusively metabolized by conjugation with glucuronic acid. Comparing the plasma clearance rates and the plasma half lives of the two drugs, correlation coefficients of r = 0.766 (P less than 0.01) and r = 0.966 (P less than 0.01) have been obtained. The data suggests that in man the processes of oxidation and glucuronidation are linked by a common control which regulates the metabolic rates of the two reactions.", "contents": "On the regulation of antipyrine and oxazepam metabolism in man. The metabolism of antipyrine and oxazepam has been measured in twelve healthy subjects. Antipyrine, an analgesic and antipyretic drug, is extensively metabolized by oxidation at various sites and oxazepam, a benzodiazepine, is almost exclusively metabolized by conjugation with glucuronic acid. Comparing the plasma clearance rates and the plasma half lives of the two drugs, correlation coefficients of r = 0.766 (P less than 0.01) and r = 0.966 (P less than 0.01) have been obtained. The data suggests that in man the processes of oxidation and glucuronidation are linked by a common control which regulates the metabolic rates of the two reactions."}
{"id": "PMID:461955", "title": "Induction of NADH-dependent aldehyde reductase by successive administration of barbiturates in rat brain.", "content": "The effect of long-term administration of phenobarbital (PB) or barbital for five weeks on brain aldehyde reductase (A1R) and aldehyde dehydrogenase (A1DH) activities in the rat was studied. Mitochondrial (m)-A1DH and NADH-dependent A1R activities were significantly increased over control values after five-week treatment with PB or barbital, while no significant alteration of supernatant (s)-A1DH and NADPH-dependent A1R activities was observed under the same condition. Increase in m-A1DH activity by the treatment with barbiturates was recovered to the control level, however, increased activity of NADH-dependent A1R was maintained even after the cessation of the treatment. In groups of rats pretreated with barbiturates for five weeks, no animals were induced to sleep after intracerebroventricular injection of PB, and this finding strongly suggests the decrease in sensitivity of rats to barbiturates.", "contents": "Induction of NADH-dependent aldehyde reductase by successive administration of barbiturates in rat brain. The effect of long-term administration of phenobarbital (PB) or barbital for five weeks on brain aldehyde reductase (A1R) and aldehyde dehydrogenase (A1DH) activities in the rat was studied. Mitochondrial (m)-A1DH and NADH-dependent A1R activities were significantly increased over control values after five-week treatment with PB or barbital, while no significant alteration of supernatant (s)-A1DH and NADPH-dependent A1R activities was observed under the same condition. Increase in m-A1DH activity by the treatment with barbiturates was recovered to the control level, however, increased activity of NADH-dependent A1R was maintained even after the cessation of the treatment. In groups of rats pretreated with barbiturates for five weeks, no animals were induced to sleep after intracerebroventricular injection of PB, and this finding strongly suggests the decrease in sensitivity of rats to barbiturates."}
{"id": "PMID:461956", "title": "Effects of cannabinoids on L1210 murine leukemia. III. Inhibition of respiration.", "content": "The present study was undertaken in an attempt to establish whether or not some of the many biochemical effects of cannabinoids could be explained by inhibition of energy generation. Of particular interest was the relationship of inhibition of DNA synthesis in L1210 murine leukemia cells by certain cannabinoids to possible effects on respiration. In studies using these cells, delta9-tetrahydrocannabinol (delta9-THC) and delta8-tetrahydrocannabinol (delta8-THC) were inactive when tested both in vitro and in vivo. Since these cannabinoids have previously been shown to be inhibitors of DNA synthesis in L1210 cells, there is no necessary relationship between inhibition of the two processes.", "contents": "Effects of cannabinoids on L1210 murine leukemia. III. Inhibition of respiration. The present study was undertaken in an attempt to establish whether or not some of the many biochemical effects of cannabinoids could be explained by inhibition of energy generation. Of particular interest was the relationship of inhibition of DNA synthesis in L1210 murine leukemia cells by certain cannabinoids to possible effects on respiration. In studies using these cells, delta9-tetrahydrocannabinol (delta9-THC) and delta8-tetrahydrocannabinol (delta8-THC) were inactive when tested both in vitro and in vivo. Since these cannabinoids have previously been shown to be inhibitors of DNA synthesis in L1210 cells, there is no necessary relationship between inhibition of the two processes."}
{"id": "PMID:461957", "title": "Studies on the relationship between ST-segment elevations and extent of infarction following coronary artery occlusion in dogs.", "content": "Epicardial ST-segment alterations were determined 15 min. following acute ligation of the left anterior descending coronary artery in dogs, and the extent of the left ventricular wall infarcted at each mapping site was determined 24 hours later. A close correlation was observed between the ST-segment elevation and the percent of left ventricular wall infarcted (r = .891; P less than .001) at the same mapping site. A significant portion of the left ventricular wall remained viable at sites with less than 8 mV elevation; however, the chance of transmural infarction became high at sites with elevations greater than 10 mV. It may be that sites with less than 10 mV elevation in the epicardial ST-segment elevation are ischemic areas where pharmacologic interventions designed to limit infarct size have the greatest change of success.", "contents": "Studies on the relationship between ST-segment elevations and extent of infarction following coronary artery occlusion in dogs. Epicardial ST-segment alterations were determined 15 min. following acute ligation of the left anterior descending coronary artery in dogs, and the extent of the left ventricular wall infarcted at each mapping site was determined 24 hours later. A close correlation was observed between the ST-segment elevation and the percent of left ventricular wall infarcted (r = .891; P less than .001) at the same mapping site. A significant portion of the left ventricular wall remained viable at sites with less than 8 mV elevation; however, the chance of transmural infarction became high at sites with elevations greater than 10 mV. It may be that sites with less than 10 mV elevation in the epicardial ST-segment elevation are ischemic areas where pharmacologic interventions designed to limit infarct size have the greatest change of success."}
{"id": "PMID:461958", "title": "Use of structure-activity relationships to estimate antiallergic activity of 4-hydroxy 3-nitro coumarins.", "content": "The antiallergic activity of a series of 4-hydroxy 3-nitro coumarins was studied using Hansch equations and CNDO/2 calculations. Following these calculations a new compound was synthetised which presents an interesting activity.", "contents": "Use of structure-activity relationships to estimate antiallergic activity of 4-hydroxy 3-nitro coumarins. The antiallergic activity of a series of 4-hydroxy 3-nitro coumarins was studied using Hansch equations and CNDO/2 calculations. Following these calculations a new compound was synthetised which presents an interesting activity."}
{"id": "PMID:461959", "title": "Anticonvulsant, analgesic and monoamine oxidase inhibitory properties of newer 4-hydroxy-4-phenylpiperidinocarbamides.", "content": "Fourteen 1-[N-acetyl(4-hydroxy-4-phenyl-piperidino)]-3-aryl carbamides were synthesized, characterized and evaluated for their anticonvulsant, analgesic and monoamine oxidase inhibitory properties. All carbamides (100 mg/kg, i.p.) provided 20--60% protection against pentylenetetrazol-induced seizures in mice. The analgesic activity possessed by these carbamides, with the exception of two compounds, was reflected by their ability to provide 17--67% protection against tail pinch response in mice. All carbamides (1 mM) inhibited (5--90%) in vitro activity of rat brain monoamine oxidase. The low toxicity of these carbamides was reflected by their higher approximate LD50 values which ranged from 500 - greater than 1000 mg/kg.", "contents": "Anticonvulsant, analgesic and monoamine oxidase inhibitory properties of newer 4-hydroxy-4-phenylpiperidinocarbamides. Fourteen 1-[N-acetyl(4-hydroxy-4-phenyl-piperidino)]-3-aryl carbamides were synthesized, characterized and evaluated for their anticonvulsant, analgesic and monoamine oxidase inhibitory properties. All carbamides (100 mg/kg, i.p.) provided 20--60% protection against pentylenetetrazol-induced seizures in mice. The analgesic activity possessed by these carbamides, with the exception of two compounds, was reflected by their ability to provide 17--67% protection against tail pinch response in mice. All carbamides (1 mM) inhibited (5--90%) in vitro activity of rat brain monoamine oxidase. The low toxicity of these carbamides was reflected by their higher approximate LD50 values which ranged from 500 - greater than 1000 mg/kg."}
{"id": "PMID:461960", "title": "Synthesis and biological activity evaluation of some quinolines.", "content": "Synthesis of some quinolines having the thiosemicarbazide and nitrostyryl moieties is described. 8-nitroquinoline-4-acraldehyde undergoes Vilsmeier reaction with dimethylacetamide to afford the respective pentadiene amide. Screening of the synthesized quinolines for bactericidal and mollusicidal activities is reported.", "contents": "Synthesis and biological activity evaluation of some quinolines. Synthesis of some quinolines having the thiosemicarbazide and nitrostyryl moieties is described. 8-nitroquinoline-4-acraldehyde undergoes Vilsmeier reaction with dimethylacetamide to afford the respective pentadiene amide. Screening of the synthesized quinolines for bactericidal and mollusicidal activities is reported."}
{"id": "PMID:461961", "title": "Synthesis and biological activity of some quinoline acrylamides.", "content": "Synthesis of some quinoline acrylamides is described. The NMR spectra of some representatives are discussed. Screening of the synthesized compounds for molluscicidal and bactericidal activity is commented.", "contents": "Synthesis and biological activity of some quinoline acrylamides. Synthesis of some quinoline acrylamides is described. The NMR spectra of some representatives are discussed. Screening of the synthesized compounds for molluscicidal and bactericidal activity is commented."}
{"id": "PMID:461962", "title": "Myocardial enzyme activities in congestive cardiomyopathy.", "content": "A number of enzyme activities concerned with various catabolic pathways have been measured in biopsy specimens of the left ventricle in patients with congestive cardiomyopathy and compared with those in patients undergoing surgery for valvar or congenital heart disease. The myopathic patients showed a diminution in glycolytic capacity, in hexokinase activity and in the capacity to transfer reducing equivalents across the mitochondrial membrane.", "contents": "Myocardial enzyme activities in congestive cardiomyopathy. A number of enzyme activities concerned with various catabolic pathways have been measured in biopsy specimens of the left ventricle in patients with congestive cardiomyopathy and compared with those in patients undergoing surgery for valvar or congenital heart disease. The myopathic patients showed a diminution in glycolytic capacity, in hexokinase activity and in the capacity to transfer reducing equivalents across the mitochondrial membrane."}
{"id": "PMID:461963", "title": "Fatty acid changes in the hepatic endoplasmic reticulum during pregnancy in the rat.", "content": "In the rat, pregnancy caused a significant reduction of total hepatic and microsomal phospholipid content mainly manifesting in phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine fractions. Fatty acid content of the liver and microsomes was also reduced. The major components involved in this change were palmitic, stearic, oleic, linoleic and arachidonic acids. The level of docosahexaenoic acid was raised; the other acyl components remained unaltered. All these chnages were reversible; both hepatic and microsomal phospholipids and fatty acids returned to the non-pregnany level 2--3 weeks after delivery.", "contents": "Fatty acid changes in the hepatic endoplasmic reticulum during pregnancy in the rat. In the rat, pregnancy caused a significant reduction of total hepatic and microsomal phospholipid content mainly manifesting in phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine fractions. Fatty acid content of the liver and microsomes was also reduced. The major components involved in this change were palmitic, stearic, oleic, linoleic and arachidonic acids. The level of docosahexaenoic acid was raised; the other acyl components remained unaltered. All these chnages were reversible; both hepatic and microsomal phospholipids and fatty acids returned to the non-pregnany level 2--3 weeks after delivery."}
{"id": "PMID:461964", "title": "Effect of exercise stress upon the acute toxicity of adriamycin in mice.", "content": "The effect of an acute exercise stress upon the acute toxicity of adriamycin was tested. Mice were forced to swim for 30 min following 18 or 23 mg/kg ip of adriamycin. Survival was checked daily for 30 days. The exercise stress did not appear to increase the toxicity of adriamycin in these animals.", "contents": "Effect of exercise stress upon the acute toxicity of adriamycin in mice. The effect of an acute exercise stress upon the acute toxicity of adriamycin was tested. Mice were forced to swim for 30 min following 18 or 23 mg/kg ip of adriamycin. Survival was checked daily for 30 days. The exercise stress did not appear to increase the toxicity of adriamycin in these animals."}
{"id": "PMID:461965", "title": "Augmentation of hemagglutination-inhibition (HI) antibodies in gilts of breeding age by large dosages of human A/New Jersey/76 influenza vaccine.", "content": "Fifteen gilts of breeding age were studied. Four received three 4 ml.  doses three weeks apart of an experimental killed swine influenza vaccine containing A/Swine/ill/65 antigen, four received three ml. doses three weeks apart of a human influenza vaccine containing A/New Jersey/76 antigen and three received two doses of human influenza vaccine followed by a 3 ml. third dose of experimental swine influenza vaccine. All except one vaccinate responded to two doses of vaccine by the time of the third dose of vaccine. HI titers were highest four weeks after the third dose of vaccine. Geometric mean titers were highest in animals receiving human influenza vaccine containing A/New Jersey/76. Titer profiles dropped markedly by 60 days after the third vaccination.", "contents": "Augmentation of hemagglutination-inhibition (HI) antibodies in gilts of breeding age by large dosages of human A/New Jersey/76 influenza vaccine. Fifteen gilts of breeding age were studied. Four received three 4 ml.  doses three weeks apart of an experimental killed swine influenza vaccine containing A/Swine/ill/65 antigen, four received three ml. doses three weeks apart of a human influenza vaccine containing A/New Jersey/76 antigen and three received two doses of human influenza vaccine followed by a 3 ml. third dose of experimental swine influenza vaccine. All except one vaccinate responded to two doses of vaccine by the time of the third dose of vaccine. HI titers were highest four weeks after the third dose of vaccine. Geometric mean titers were highest in animals receiving human influenza vaccine containing A/New Jersey/76. Titer profiles dropped markedly by 60 days after the third vaccination."}
{"id": "PMID:461966", "title": "Effect of an essential fatty acid deficient diet on dimethylnitrosamine demethylation and derangement of liver polyribosomes induced by this drug.", "content": "Cytochrome P-450 content and dimethylnitrosamine demethylase activity were determined in the liver of male rats fed a polyunsaturated fatty acid deficient diet. The damaging activity of dimethylnitrosamine (DMN) on liver polyribosomes of control and treated rats was estimated. The reduction of DMN demethylase activity did not protect against polysome derangement due to DMN. These data are consistent with the hypothesis that metabolic pathways, other than the P-450 dependent one, are involved in the DMN bioactivation.", "contents": "Effect of an essential fatty acid deficient diet on dimethylnitrosamine demethylation and derangement of liver polyribosomes induced by this drug. Cytochrome P-450 content and dimethylnitrosamine demethylase activity were determined in the liver of male rats fed a polyunsaturated fatty acid deficient diet. The damaging activity of dimethylnitrosamine (DMN) on liver polyribosomes of control and treated rats was estimated. The reduction of DMN demethylase activity did not protect against polysome derangement due to DMN. These data are consistent with the hypothesis that metabolic pathways, other than the P-450 dependent one, are involved in the DMN bioactivation."}
{"id": "PMID:461967", "title": "Inability of imipramine to protect against type A or type B monoamine oxidase inhibition by pargyline.", "content": "Imipramine (30 mg/kg i.p.) did not antagonize the inhibition of either type A or type B MAO by pargyline in brain, heart or liver of rats. Occupancy of active sites on mitochondrial MAO by imipramine probably does not occur at doses of imipramine adequate to inhibit monoamine uptake.", "contents": "Inability of imipramine to protect against type A or type B monoamine oxidase inhibition by pargyline. Imipramine (30 mg/kg i.p.) did not antagonize the inhibition of either type A or type B MAO by pargyline in brain, heart or liver of rats. Occupancy of active sites on mitochondrial MAO by imipramine probably does not occur at doses of imipramine adequate to inhibit monoamine uptake."}
{"id": "PMID:461968", "title": "Behavioral and biochemical effects of para-methoxyphenylethylamine.", "content": "p-methoxyphenlethylamine (p-MPEA) induces the \"serotonin syndrome\" in mice which consists of lateral head weaving, Straub tail, hindlimb abduction, tremor, hyperactivity, reciprocal fore-paw treading, salivation and piloerection. These p-MPEA-induced behavioral signs were partially prevented by pretreatment with serotonin (5HT) uptake blockers (fluoxetine, chlorimipramine, Org 6582) and 5HT receptor blockers (methiothepin, methysergide, cinnanserin) but not by two depletors of brain 5HT (p-chlorophenylalanine, reserpine). p-MPEA selectively released 5HT and inhibited 5HT uptake from mouse whole brain synaptosomes. The concentrations of both 5HT and 5-hydroxyindoleacetic acid (5HIAA) in whole mouse brain were increased at 1 and 2 hours after injection of p-MPEA (50 mg/kg). The effect of p-MPEA on displacement of specific tritiated 5HT binding to membranes from mouse brain was considerably less than that of p-chlorophenylethylamine (p-CPEA), p-methoxytryptamine (pMT) and quipazine. Thus p-MPEA may induce the \"serotonin syndrome\" predominantly by releasing 5HT from presynaptic nerve terminals.", "contents": "Behavioral and biochemical effects of para-methoxyphenylethylamine. p-methoxyphenlethylamine (p-MPEA) induces the \"serotonin syndrome\" in mice which consists of lateral head weaving, Straub tail, hindlimb abduction, tremor, hyperactivity, reciprocal fore-paw treading, salivation and piloerection. These p-MPEA-induced behavioral signs were partially prevented by pretreatment with serotonin (5HT) uptake blockers (fluoxetine, chlorimipramine, Org 6582) and 5HT receptor blockers (methiothepin, methysergide, cinnanserin) but not by two depletors of brain 5HT (p-chlorophenylalanine, reserpine). p-MPEA selectively released 5HT and inhibited 5HT uptake from mouse whole brain synaptosomes. The concentrations of both 5HT and 5-hydroxyindoleacetic acid (5HIAA) in whole mouse brain were increased at 1 and 2 hours after injection of p-MPEA (50 mg/kg). The effect of p-MPEA on displacement of specific tritiated 5HT binding to membranes from mouse brain was considerably less than that of p-chlorophenylethylamine (p-CPEA), p-methoxytryptamine (pMT) and quipazine. Thus p-MPEA may induce the \"serotonin syndrome\" predominantly by releasing 5HT from presynaptic nerve terminals."}
{"id": "PMID:461969", "title": "Depressive action of clonidine on guinea pig and rabbit atrial pairs.", "content": "The effects of clonidine, a compound used clinically as a centrally active antihypertensive, were studied on isolated, spontaneously beating atria of rabbit and guinea pig. In rabbit atria clonidine induces a marked depression of spontaneous chronotropic activity which was not altered by pretreatment with atropine, and not completely reversed by washing. In guinea pig atria, clonidine alone induces a very small increase in rate. A significant decrease in rate however is unmasked when tissues are pretreated with metiamide (H2-antagonist). Since both rabbit and guinea pig atria contain histamine receptors the lack of a positive chronotropic response in rabbit atria suggests that clonidine has no direct H2-receptor activity. The small positive response obtained with guinea pig atria which can be blocked by metiamide suggests that clonidine can act indirectly by releasing endogenous histamine stores from this tissue. Clonidines direct action appears to be a significant depression of both rabbit and guinea pig atrial chronotropic activity.", "contents": "Depressive action of clonidine on guinea pig and rabbit atrial pairs. The effects of clonidine, a compound used clinically as a centrally active antihypertensive, were studied on isolated, spontaneously beating atria of rabbit and guinea pig. In rabbit atria clonidine induces a marked depression of spontaneous chronotropic activity which was not altered by pretreatment with atropine, and not completely reversed by washing. In guinea pig atria, clonidine alone induces a very small increase in rate. A significant decrease in rate however is unmasked when tissues are pretreated with metiamide (H2-antagonist). Since both rabbit and guinea pig atria contain histamine receptors the lack of a positive chronotropic response in rabbit atria suggests that clonidine has no direct H2-receptor activity. The small positive response obtained with guinea pig atria which can be blocked by metiamide suggests that clonidine can act indirectly by releasing endogenous histamine stores from this tissue. Clonidines direct action appears to be a significant depression of both rabbit and guinea pig atrial chronotropic activity."}
{"id": "PMID:461970", "title": "Stereoselective oral bioavailability of (+/-)-propranolol in the dog. A GC-MS study using a stable isotope technique.", "content": "The disposition of (+)- and (-)-propranolol and their glucuronic acid conjugates, (+)-POG and (-)-POG, in plasma was determined in 5 dogs following single 160 mg oral doses of stable isotope labeled racemic propranolol. Each animal received one dose of the racemate with the (+)-isomer labeled with two deuteriums and one dose with the (-)-isomer labeled. The isomers were separated and measured by gas chromatography-mass spectrometry. No stable isotope effect was detected. The area under the plasma concentration - time curve (AUC) for (-)-propranolol was only 54 +/- 10 per cent (mean +/- SD; n = 10) of the AUC for (+)-propranolol. The AUC of (-)-POG exceeded the AUC of (+)-POG by 4.02 +/- 1.22 times (mean +/- SD: n = 10). The time to peak plasma concentration (3.9-4.3 hr) and the half-life (1.6-1.9 hr) were identical for both isomers of propranolol as well as of POG. These results demonstrate a significantly lower oral bioavailability of (-)- as compared to (+)-propranolol in the dog, which appears to be associated with stereoselective presystemic glucuronidation of (-)-propranolol.", "contents": "Stereoselective oral bioavailability of (+/-)-propranolol in the dog. A GC-MS study using a stable isotope technique. The disposition of (+)- and (-)-propranolol and their glucuronic acid conjugates, (+)-POG and (-)-POG, in plasma was determined in 5 dogs following single 160 mg oral doses of stable isotope labeled racemic propranolol. Each animal received one dose of the racemate with the (+)-isomer labeled with two deuteriums and one dose with the (-)-isomer labeled. The isomers were separated and measured by gas chromatography-mass spectrometry. No stable isotope effect was detected. The area under the plasma concentration - time curve (AUC) for (-)-propranolol was only 54 +/- 10 per cent (mean +/- SD; n = 10) of the AUC for (+)-propranolol. The AUC of (-)-POG exceeded the AUC of (+)-POG by 4.02 +/- 1.22 times (mean +/- SD: n = 10). The time to peak plasma concentration (3.9-4.3 hr) and the half-life (1.6-1.9 hr) were identical for both isomers of propranolol as well as of POG. These results demonstrate a significantly lower oral bioavailability of (-)- as compared to (+)-propranolol in the dog, which appears to be associated with stereoselective presystemic glucuronidation of (-)-propranolol."}
{"id": "PMID:461971", "title": "Inhibitory effect of quinidine on catecholamine release from adrenal medulla.", "content": "Quinidine was found to inhibit catecholamine release from the medulla of perfused adrenal glands induced by carbamylcholine. It also inhibited the release of catecholamine from adrenomedullary granules induced by ATP-Mg++.", "contents": "Inhibitory effect of quinidine on catecholamine release from adrenal medulla. Quinidine was found to inhibit catecholamine release from the medulla of perfused adrenal glands induced by carbamylcholine. It also inhibited the release of catecholamine from adrenomedullary granules induced by ATP-Mg++."}
{"id": "PMID:461973", "title": "Saliva, urine and plasma analysis of dyphylline via HPLC.", "content": "Improved assay methods for determination of dyphylline levels in plasma, saliva, and urine utilizing high pressure liquid chromatography have been developed. Detection of levels as low as 25 ng/ml from 0.5 ml plasma and 50 ng/ml from 0.5 ml saliva were possible. Both procedures utilized the same extraction process. A separate extraction process was used for dyphylline analysis in urine due to the presence of interferring substances occurring with previous methods. The method was applied to samples of one subjects plasma, saliva and urine after administration of dyphylline.", "contents": "Saliva, urine and plasma analysis of dyphylline via HPLC. Improved assay methods for determination of dyphylline levels in plasma, saliva, and urine utilizing high pressure liquid chromatography have been developed. Detection of levels as low as 25 ng/ml from 0.5 ml plasma and 50 ng/ml from 0.5 ml saliva were possible. Both procedures utilized the same extraction process. A separate extraction process was used for dyphylline analysis in urine due to the presence of interferring substances occurring with previous methods. The method was applied to samples of one subjects plasma, saliva and urine after administration of dyphylline."}
{"id": "PMID:461974", "title": "Rapid determination of serum theophylline levels using the MPLC short column concept.", "content": "Many procedures are currently available demonstrating the feasibility of the analysis of serum theophylline concentrations by high performance, high pressure liquid chromatography utilizing chromatographic columns which can only be described by the term \"expensive\" in terms of column life and cost. We have developed and evaluated a rapid \"medium pressure, medium performance\" liquid chromatographic (MPLC) method for theophylline in serum utilizing a chromatographic column that costs up to 1/3 the cost of current HPLC columns. Less than 0.2 ml of serum is required for each determination, yet the sensitivity of the method is easily less than 1 mg/liter. The method involves liquid extractive isolation, chromatography on a micro column (4.6mm x 10cm) of RP-18 and provides adequate selectivity, accuracy and precision for routine and non-routine (neonatal) theophylline determinations. We have found no endogenous or exogenous interferences. Reproducibility for the microscale method is controlled through the use of hydroxypropyl theophylline as an internal standard.", "contents": "Rapid determination of serum theophylline levels using the MPLC short column concept. Many procedures are currently available demonstrating the feasibility of the analysis of serum theophylline concentrations by high performance, high pressure liquid chromatography utilizing chromatographic columns which can only be described by the term \"expensive\" in terms of column life and cost. We have developed and evaluated a rapid \"medium pressure, medium performance\" liquid chromatographic (MPLC) method for theophylline in serum utilizing a chromatographic column that costs up to 1/3 the cost of current HPLC columns. Less than 0.2 ml of serum is required for each determination, yet the sensitivity of the method is easily less than 1 mg/liter. The method involves liquid extractive isolation, chromatography on a micro column (4.6mm x 10cm) of RP-18 and provides adequate selectivity, accuracy and precision for routine and non-routine (neonatal) theophylline determinations. We have found no endogenous or exogenous interferences. Reproducibility for the microscale method is controlled through the use of hydroxypropyl theophylline as an internal standard."}
{"id": "PMID:461975", "title": "Purification of rabbit antispermine antiserum by affinity chromatography.", "content": "Immunoglobulins G were isolated from a crude rabbit antispermine antiserum using a protein A-Sepharose CL-4B column. Affinity chromatography coupling spermine to the Sepharose matrix was employed for separation of antispermine antibodies from these immunoglobulins. Four fractions of antispermine antibodies were isolated by stepwise elution with solutions at pH range from 4 to 1. Binding constants were determined and cross-reactivity with other polyamines was tested for each fraction of antibodies. Highly specific antispermine antibodies were found in fraction IV.", "contents": "Purification of rabbit antispermine antiserum by affinity chromatography. Immunoglobulins G were isolated from a crude rabbit antispermine antiserum using a protein A-Sepharose CL-4B column. Affinity chromatography coupling spermine to the Sepharose matrix was employed for separation of antispermine antibodies from these immunoglobulins. Four fractions of antispermine antibodies were isolated by stepwise elution with solutions at pH range from 4 to 1. Binding constants were determined and cross-reactivity with other polyamines was tested for each fraction of antibodies. Highly specific antispermine antibodies were found in fraction IV."}
{"id": "PMID:461976", "title": "The fate of intratracheally instilled 3H-styrene oxide in the isolated perfused rat lungs.", "content": "The metabolism and covalent binding of intratracheally instilled 7-3H-styrene oxide in the isolated perfused lungs of control and cigarette smoke-exposed rats were investigated. After intratracheal instillation of 100 nmoles of 7-3H-styrene oxide the lungs were perfused for 16 min and the nonrecirculating perfusate was collected in two fractions: 0--8 min and 8--16 min. About 40 percent of the instilled dose was in the 0--8 min perfusion medium, mainly in the form of unmetabolized styrene oxide. However, the radioactivity in the 8--16 min perfusion medium and lung tissue itself was mainly in the form of water soluble metabolites, probably glutathione conjugates. The amount of styrene glycol was very small. Exposure of rat to cigarette smoke had no effect on the metabolism of styrene oxide or on the amount of covalent binding to the protein and nucleic acid fractions of the perfused lungs.", "contents": "The fate of intratracheally instilled 3H-styrene oxide in the isolated perfused rat lungs. The metabolism and covalent binding of intratracheally instilled 7-3H-styrene oxide in the isolated perfused lungs of control and cigarette smoke-exposed rats were investigated. After intratracheal instillation of 100 nmoles of 7-3H-styrene oxide the lungs were perfused for 16 min and the nonrecirculating perfusate was collected in two fractions: 0--8 min and 8--16 min. About 40 percent of the instilled dose was in the 0--8 min perfusion medium, mainly in the form of unmetabolized styrene oxide. However, the radioactivity in the 8--16 min perfusion medium and lung tissue itself was mainly in the form of water soluble metabolites, probably glutathione conjugates. The amount of styrene glycol was very small. Exposure of rat to cigarette smoke had no effect on the metabolism of styrene oxide or on the amount of covalent binding to the protein and nucleic acid fractions of the perfused lungs."}
{"id": "PMID:461977", "title": "Long-term estrogenization in mammals. I. Histopathology of kidney, bladder, adrenals, and gonads; polydipsia; body weight; and serum levels of corticosterone and testosterone in estrogenized Marsh mice.", "content": "Single, sublethal, long-acting doses of estrone or estradiol cypionate produced the following histologic, behavioral, and blood chemistry effects in Marsh mice: (a) Bladder pathology developed following estrogen-induced polydipsia but was not consistently associated with kidney damage. (b) This polydipsia was more intense (a maximum of 250% over control intake) when the housing was in metal cages with floor screens than in plastic cages with bedding. Water intake of control mice was unaffected by housing changes. (c) Comparing litter-mate 2.5-month-old males and females, body weight loss following estrogen administration was transient with the females. With males, initial weight losses as compared with weight gains in the controls were demonstrated over a 20-week period. (d) Estrogenization markedly increased corticosterone and decreased testosterone serum levels with a significant negative correlation for these levels in the estrogenized mice. A significant positive correlation was also observed between testosterone levels and testis weights in the estrogenized mice.", "contents": "Long-term estrogenization in mammals. I. Histopathology of kidney, bladder, adrenals, and gonads; polydipsia; body weight; and serum levels of corticosterone and testosterone in estrogenized Marsh mice. Single, sublethal, long-acting doses of estrone or estradiol cypionate produced the following histologic, behavioral, and blood chemistry effects in Marsh mice: (a) Bladder pathology developed following estrogen-induced polydipsia but was not consistently associated with kidney damage. (b) This polydipsia was more intense (a maximum of 250% over control intake) when the housing was in metal cages with floor screens than in plastic cages with bedding. Water intake of control mice was unaffected by housing changes. (c) Comparing litter-mate 2.5-month-old males and females, body weight loss following estrogen administration was transient with the females. With males, initial weight losses as compared with weight gains in the controls were demonstrated over a 20-week period. (d) Estrogenization markedly increased corticosterone and decreased testosterone serum levels with a significant negative correlation for these levels in the estrogenized mice. A significant positive correlation was also observed between testosterone levels and testis weights in the estrogenized mice."}
{"id": "PMID:461978", "title": "Effects of pesticides on human leukocyte functions.", "content": "Aroclor 1254 and twelve pesticides of different chemical classes were tested for their in vitro effects on lymphocyte mitogenic responses to PHA, rosette formation with sheep red blood cells (E-rosette) and neutrophil chemotaxis. By using a diluted whole blood culture, butoxide at 10 micrometers inhibit lymphocyte mitogenic response to PHA by 26%. Inhibition by other pesticides were less than 20% (5--20(). Higher degrees of inhibition were observed if the lymphocytes were pretreated with pesticides in the absence of serum: pp-DDT inhibited by 73% trithion by 50% and benzylthiocyanate by 30%. E-rosette formation was less inhibited by these pesticides except DDT, which inhibited the E-rosette formation by 50% at 10(-4)M. Only methyl parathion was found to have some inhibitory effects on neutrophil chemotaxis. These results indicate that insecticides may have direct effects on the leukocyte function and that the presence of serum may alter the interaction of insecticides and leukocyte membranes.", "contents": "Effects of pesticides on human leukocyte functions. Aroclor 1254 and twelve pesticides of different chemical classes were tested for their in vitro effects on lymphocyte mitogenic responses to PHA, rosette formation with sheep red blood cells (E-rosette) and neutrophil chemotaxis. By using a diluted whole blood culture, butoxide at 10 micrometers inhibit lymphocyte mitogenic response to PHA by 26%. Inhibition by other pesticides were less than 20% (5--20(). Higher degrees of inhibition were observed if the lymphocytes were pretreated with pesticides in the absence of serum: pp-DDT inhibited by 73% trithion by 50% and benzylthiocyanate by 30%. E-rosette formation was less inhibited by these pesticides except DDT, which inhibited the E-rosette formation by 50% at 10(-4)M. Only methyl parathion was found to have some inhibitory effects on neutrophil chemotaxis. These results indicate that insecticides may have direct effects on the leukocyte function and that the presence of serum may alter the interaction of insecticides and leukocyte membranes."}
{"id": "PMID:461979", "title": "Effect of furosemide on canine splenic arterial blood flow.", "content": "Furosemide has been reported to cause pathological changes in the intestines as a consequence of the decrease in splanchnic blood flow that it produces. In view of the pancreatic toxicities of this diuretic, it was of interest to examine the effect of the agent on blood flow to this organ. An electromagnetic flow probe, was placed around the splenic artery, a vessel which supplies a major fraction of pancreatic blood flow. Within 60 min after furosemide (1 mg/kg, iv) administration, splenic blood flow (SBF) decreased by 20% and the decrease paralleled the drug-induced diuresis. When the diuresis was prevented, the agent failed to affect SBF. Thus, furosemide causes a reduction in SBF, which appears to involve a mechanism dependent upon the volume reduction produced by the drug-induced diuresis.", "contents": "Effect of furosemide on canine splenic arterial blood flow. Furosemide has been reported to cause pathological changes in the intestines as a consequence of the decrease in splanchnic blood flow that it produces. In view of the pancreatic toxicities of this diuretic, it was of interest to examine the effect of the agent on blood flow to this organ. An electromagnetic flow probe, was placed around the splenic artery, a vessel which supplies a major fraction of pancreatic blood flow. Within 60 min after furosemide (1 mg/kg, iv) administration, splenic blood flow (SBF) decreased by 20% and the decrease paralleled the drug-induced diuresis. When the diuresis was prevented, the agent failed to affect SBF. Thus, furosemide causes a reduction in SBF, which appears to involve a mechanism dependent upon the volume reduction produced by the drug-induced diuresis."}
{"id": "PMID:461981", "title": "A histochemical technique for evaluation of progesterone receptors in breast cancer.", "content": "A histochemical method for the detection and localization of progesterone receptors in human breast cancer has been developed employing a fluorescein labeled conjugate of bovine serum albumin linked to a progestin as the binding hormone. Considerable tumor cell receptor heterogeneity was apparent and nuclear binding was frequently noted. The results of the new assay correlated with those obtained by dextran-coated charcoal assay in 91 per cent of specimens.", "contents": "A histochemical technique for evaluation of progesterone receptors in breast cancer. A histochemical method for the detection and localization of progesterone receptors in human breast cancer has been developed employing a fluorescein labeled conjugate of bovine serum albumin linked to a progestin as the binding hormone. Considerable tumor cell receptor heterogeneity was apparent and nuclear binding was frequently noted. The results of the new assay correlated with those obtained by dextran-coated charcoal assay in 91 per cent of specimens."}
{"id": "PMID:461982", "title": "Hepatic RNA synthesis in rats treated with ethylene thiourea.", "content": "The ability of ethylene thiourea (ETU) to inhibit RNA synthesis in rat liver was investigated. This compound, a possibly carcinogenic metabolite to the ethylene-bis-dithiocarbamate fungicides, was administered to rats by intraperitoneal injection, by nasogastric tube and in the diet, and rates of RNA synthesis were determined. By contrast with the carcinogens thioacetamide and acetylaminofluorene, high doses of ETU administered by any of these routes failed to inhibit the synthesis of nuclear or cytoplasmic RNA. In this respect ETU appears to differ from most hepatocarcinogens in its effect on cellular metabolism.", "contents": "Hepatic RNA synthesis in rats treated with ethylene thiourea. The ability of ethylene thiourea (ETU) to inhibit RNA synthesis in rat liver was investigated. This compound, a possibly carcinogenic metabolite to the ethylene-bis-dithiocarbamate fungicides, was administered to rats by intraperitoneal injection, by nasogastric tube and in the diet, and rates of RNA synthesis were determined. By contrast with the carcinogens thioacetamide and acetylaminofluorene, high doses of ETU administered by any of these routes failed to inhibit the synthesis of nuclear or cytoplasmic RNA. In this respect ETU appears to differ from most hepatocarcinogens in its effect on cellular metabolism."}
{"id": "PMID:461983", "title": "Distribution of [3H]prednisolone entrapped in lipid layer of liposome after intramuscular administration in rats.", "content": "Positivery charged, sonicated liposome entrapping [3H]prednisolone was administered into the rats hip muscle to examine its absorption and distribution in comparison with that of the administration of free [3H]prednisolone. Plasma concentration of prednisolone in liposome-entrapped form showed high concentration for a long period of time, the values being 8 times after 30 min and 24 times after 24 hr compared to the free form. The concentration of liposome-entrapped form in liver, kidney and spleen was 4-10 times, and in lung and pancreas was 8-10 times higher than that of free form after 30 min and 24 hr. But, in adrenal, after 30 min and 1 hr, liposome-entrapped form were lower than that of free form, the former remained longer in the tissue, and the concentration maintained the high level after 24 hr, being about 28 times higher than that of free form. Even in the duodenum and large intestine, absorption of the liposome-entrapped form was very active and remained in the tissue for a long period of time. These facts suggested the possibility of using a small dose in intramuscular administration but allowing the steroid to remain in the tissues for a long period of time.", "contents": "Distribution of [3H]prednisolone entrapped in lipid layer of liposome after intramuscular administration in rats. Positivery charged, sonicated liposome entrapping [3H]prednisolone was administered into the rats hip muscle to examine its absorption and distribution in comparison with that of the administration of free [3H]prednisolone. Plasma concentration of prednisolone in liposome-entrapped form showed high concentration for a long period of time, the values being 8 times after 30 min and 24 times after 24 hr compared to the free form. The concentration of liposome-entrapped form in liver, kidney and spleen was 4-10 times, and in lung and pancreas was 8-10 times higher than that of free form after 30 min and 24 hr. But, in adrenal, after 30 min and 1 hr, liposome-entrapped form were lower than that of free form, the former remained longer in the tissue, and the concentration maintained the high level after 24 hr, being about 28 times higher than that of free form. Even in the duodenum and large intestine, absorption of the liposome-entrapped form was very active and remained in the tissue for a long period of time. These facts suggested the possibility of using a small dose in intramuscular administration but allowing the steroid to remain in the tissues for a long period of time."}
{"id": "PMID:461984", "title": "The effect of harmine and other monoamine oxidase inhibitors on N-acetyltransferase activity.", "content": "Prior studies indicate that the monoamine oxidase inhibitors (MAOI) harmine and iproniazide inhibit N-acetyltransferase activity from liver. In this report we have demonstrated that harmine and harmaline are potent inhibitors of N-acetyltransferase, purified from hamster and rat liver. However, other MAOI such as deprenyl, clorgyline, methysergide, cyproheptadine, phenelzine, pargyline, methyltryptamine and tranylcypromine have either no effect, or only trivial effects on N-acetyltransferase. There is no correlation between a compound's properties as an MAO and N-acetyltransferase inhibitor. One must consider the inhibitory effect of harmine and harmaline on both MAO and N-acetyltransferase when evaluating the effects of these compounds on physiological processes.", "contents": "The effect of harmine and other monoamine oxidase inhibitors on N-acetyltransferase activity. Prior studies indicate that the monoamine oxidase inhibitors (MAOI) harmine and iproniazide inhibit N-acetyltransferase activity from liver. In this report we have demonstrated that harmine and harmaline are potent inhibitors of N-acetyltransferase, purified from hamster and rat liver. However, other MAOI such as deprenyl, clorgyline, methysergide, cyproheptadine, phenelzine, pargyline, methyltryptamine and tranylcypromine have either no effect, or only trivial effects on N-acetyltransferase. There is no correlation between a compound's properties as an MAO and N-acetyltransferase inhibitor. One must consider the inhibitory effect of harmine and harmaline on both MAO and N-acetyltransferase when evaluating the effects of these compounds on physiological processes."}
{"id": "PMID:461985", "title": "Changes in serotonin turnover and the brain sensitivity to barbiturates by disulfiram treatment in rats.", "content": "The influence of intraperitoneal administration of disulfiram on the serotonin (5HT) turnover and the brain sensitivity to barbiturates were investigated in rats. Treatment of the animals with 200 mg/kg disulfiram resulted in the prolongation of duration of barbiturate-induced hypnosis. This indication and increment of the brain sensitivity to barbiturates after disulfiram treatment. Under the identical condition, disulfiram caused both the reduction of turnover of 5HT and the elevation of 5HT levels, although this effect was less potent than that of phenobarbital. Furthermore, simultaneous administration of disulfiram and phenobarbital resulted in the severe retardation of 5HT metabolism. These results strongly suggest that disulfiram potentiates the hypnotic action of barbiturates by altering 5HT metabolism in rat brain.", "contents": "Changes in serotonin turnover and the brain sensitivity to barbiturates by disulfiram treatment in rats. The influence of intraperitoneal administration of disulfiram on the serotonin (5HT) turnover and the brain sensitivity to barbiturates were investigated in rats. Treatment of the animals with 200 mg/kg disulfiram resulted in the prolongation of duration of barbiturate-induced hypnosis. This indication and increment of the brain sensitivity to barbiturates after disulfiram treatment. Under the identical condition, disulfiram caused both the reduction of turnover of 5HT and the elevation of 5HT levels, although this effect was less potent than that of phenobarbital. Furthermore, simultaneous administration of disulfiram and phenobarbital resulted in the severe retardation of 5HT metabolism. These results strongly suggest that disulfiram potentiates the hypnotic action of barbiturates by altering 5HT metabolism in rat brain."}
{"id": "PMID:461986", "title": "Comparative aspects of nuclear proteins and nuclear composition of cultivated embryonic, neonatal and neoplastic rat brain cells of glial origin.", "content": "Differences in nuclear composition and variations in the types of acid soluble nuclear proteins were identified when cultivated neoplastic and nontransformed rat brain cells were compared. HeLa S-3 cells were used as a biological reference in these studies. The nuclei of anaplastic glioma cells were found to contain more total nuclear protein and greater amounts of nuclear RNA, than the nuclei of nontransformed embryonic and neonatal rat brain cells. Densitometer profiles of samples developed by polyacrylamide gel electrophoresis indicated that all three major histone classes were present in the nuclei of the four different cell types examined. Each type of cell was grown in the presence of 3H-lysine or 3H-lysine plus 14C-arginine to further characterize both the histones and the acid soluble nonhistone proteins. Neoplastic and nontransformed cells contained similar quantities of histones H4, H3, H2A and H2B. In contrast, transformed cells were found to contain two dominant subspecies of lysine rich H1 histone, while only one histone H1 subcomponent was extracted from the nuclei of the embryonic and neonatal rat brain cells. Nuclei isolated from HeLa cells and anaplastic glioma cells also contained increased varieties and larger quantities of acid soluble nonhistone nuclear proteins.", "contents": "Comparative aspects of nuclear proteins and nuclear composition of cultivated embryonic, neonatal and neoplastic rat brain cells of glial origin. Differences in nuclear composition and variations in the types of acid soluble nuclear proteins were identified when cultivated neoplastic and nontransformed rat brain cells were compared. HeLa S-3 cells were used as a biological reference in these studies. The nuclei of anaplastic glioma cells were found to contain more total nuclear protein and greater amounts of nuclear RNA, than the nuclei of nontransformed embryonic and neonatal rat brain cells. Densitometer profiles of samples developed by polyacrylamide gel electrophoresis indicated that all three major histone classes were present in the nuclei of the four different cell types examined. Each type of cell was grown in the presence of 3H-lysine or 3H-lysine plus 14C-arginine to further characterize both the histones and the acid soluble nonhistone proteins. Neoplastic and nontransformed cells contained similar quantities of histones H4, H3, H2A and H2B. In contrast, transformed cells were found to contain two dominant subspecies of lysine rich H1 histone, while only one histone H1 subcomponent was extracted from the nuclei of the embryonic and neonatal rat brain cells. Nuclei isolated from HeLa cells and anaplastic glioma cells also contained increased varieties and larger quantities of acid soluble nonhistone nuclear proteins."}
{"id": "PMID:461987", "title": "Suppression of cellular immune response in chemically sympathectomized mice.", "content": "Delayed-type hypersensitivity to methylated bovine serum albumin (MBSA), as induced by 6-hydroxydopamine (6-OHDA) was studied in chemically sympathectomized mice. Footpad reaction at 48 h after challenge injection of MBSA was suppressed with doses ranging from 10--300 mg/kg. 6-OHDA was neither cytotoxic to peritoneal macrophages nor was the function of immunoglobulin mediated phagocytosis suppressed. Thus, the suppression of delayed-type hypersensitivity may be attributed to interference with T-cell function.", "contents": "Suppression of cellular immune response in chemically sympathectomized mice. Delayed-type hypersensitivity to methylated bovine serum albumin (MBSA), as induced by 6-hydroxydopamine (6-OHDA) was studied in chemically sympathectomized mice. Footpad reaction at 48 h after challenge injection of MBSA was suppressed with doses ranging from 10--300 mg/kg. 6-OHDA was neither cytotoxic to peritoneal macrophages nor was the function of immunoglobulin mediated phagocytosis suppressed. Thus, the suppression of delayed-type hypersensitivity may be attributed to interference with T-cell function."}
{"id": "PMID:461988", "title": "The biphasic nature and temperature dependence of the activation of human plasminogen by urokinase. A simple urokinase assay based on this phenomenon.", "content": "Activation of plasminogen is a biphasic process, the first phase is temperature independent, the second phase is not. The first phase of activation is completed immediately when urokinase (UK) is combined with plasminogen. At 4 degrees C the fibrinolytic activity generated in the first phase is directly proportional to the concentration of UK used for activation. This linear relationship permits the construction of a standard curve for the quantitative measurement of UK in samples with unknown concentrations of UK. The second, slower phase of activation follows the first only at higher temperatures (e.g. 28 degrees C), is limited by the plasminogen concentration, and is completed within 30 minutes of incubations. Activation of plasminogen by streptokinase under the same conditions follows a different pattern.", "contents": "The biphasic nature and temperature dependence of the activation of human plasminogen by urokinase. A simple urokinase assay based on this phenomenon. Activation of plasminogen is a biphasic process, the first phase is temperature independent, the second phase is not. The first phase of activation is completed immediately when urokinase (UK) is combined with plasminogen. At 4 degrees C the fibrinolytic activity generated in the first phase is directly proportional to the concentration of UK used for activation. This linear relationship permits the construction of a standard curve for the quantitative measurement of UK in samples with unknown concentrations of UK. The second, slower phase of activation follows the first only at higher temperatures (e.g. 28 degrees C), is limited by the plasminogen concentration, and is completed within 30 minutes of incubations. Activation of plasminogen by streptokinase under the same conditions follows a different pattern."}
{"id": "PMID:461989", "title": "Evaluation of various isoniazid slow releasing matrix preparations for intermittent chemotherapy of tuberculosis.", "content": "The blood level achieved with 15 mg/kg ordinary isoniazid (INH) was compared with that obtained with INH slow releasing Matrix preparation. Three brands of INH Matrix preparation were compared namely: the product of ICN Canada Ltd, utilized by Laboratory Centre for Disease Control, the preparation of Smith and Nephew, Britain employed by the Tuberculosis unit of the British Medical Research Council and Tebesium, the product of Hefa-Frenon Arzneimittel, Germany studied by the Tuberculosis Unit of South African Medical Research Council. The results of this study showed that pharmacogenetic principle have to be taken into account, it is not possible to produce INH slow releasing Matrix preparate equally applicable for slow and fast acetylators of INH.", "contents": "Evaluation of various isoniazid slow releasing matrix preparations for intermittent chemotherapy of tuberculosis. The blood level achieved with 15 mg/kg ordinary isoniazid (INH) was compared with that obtained with INH slow releasing Matrix preparation. Three brands of INH Matrix preparation were compared namely: the product of ICN Canada Ltd, utilized by Laboratory Centre for Disease Control, the preparation of Smith and Nephew, Britain employed by the Tuberculosis unit of the British Medical Research Council and Tebesium, the product of Hefa-Frenon Arzneimittel, Germany studied by the Tuberculosis Unit of South African Medical Research Council. The results of this study showed that pharmacogenetic principle have to be taken into account, it is not possible to produce INH slow releasing Matrix preparate equally applicable for slow and fast acetylators of INH."}
{"id": "PMID:461990", "title": "Time and level of perinatal lead exposure for development of norepinephrine cardiotoxicity.", "content": "Neonatal lead exposure produces an enhanced susceptibility of the adult rat heart to the arrhythmogenic effects of norepinephrine. The present study examines the relationship of time and level of exposure to the extent of cardiac abnormality produced. The results indicate that the level of lead exposure required to produce norepinephrine cardiotoxicity without overt growth retardation is quite narrow, and lead must be present during the first ten days of life to cause these effects on the heart. A critical concentration of lead achieved during the early time period after birth appears necessary for the production of this cardiac abnormality, suggesting that lead interferes with neonatal cardiac and/or autonomic development.", "contents": "Time and level of perinatal lead exposure for development of norepinephrine cardiotoxicity. Neonatal lead exposure produces an enhanced susceptibility of the adult rat heart to the arrhythmogenic effects of norepinephrine. The present study examines the relationship of time and level of exposure to the extent of cardiac abnormality produced. The results indicate that the level of lead exposure required to produce norepinephrine cardiotoxicity without overt growth retardation is quite narrow, and lead must be present during the first ten days of life to cause these effects on the heart. A critical concentration of lead achieved during the early time period after birth appears necessary for the production of this cardiac abnormality, suggesting that lead interferes with neonatal cardiac and/or autonomic development."}
{"id": "PMID:461991", "title": "Additional induction of microsomal cytochrome(s) P 450 in mouse liver by consecutive application of cadmium chloride and phenobarbital.", "content": "Mice were treated intraperitoneally over 20 days with 0,30; 0,10 and 0,03 mg Cd+2/kg.day. Subsequent induction of microsomal metabolism by 50 mg phenobarbital/kg.day over 5 days revealed increased microsomal cytochrome P 450 content in Cd+2-pretreated animals as compared to animals treated only with phenobarbital. The microsomal cadmium content of animals having received cadmium plus phenobarbital was about 5 times higher than that of animals treated with cadmium alone. The only enzyme activity found to be changed by cadmium in induced microsomes was ethylmorphine-N-deethylation. In phenobarbital-free animals no microsomal changes by cadmium could be detected.", "contents": "Additional induction of microsomal cytochrome(s) P 450 in mouse liver by consecutive application of cadmium chloride and phenobarbital. Mice were treated intraperitoneally over 20 days with 0,30; 0,10 and 0,03 mg Cd+2/kg.day. Subsequent induction of microsomal metabolism by 50 mg phenobarbital/kg.day over 5 days revealed increased microsomal cytochrome P 450 content in Cd+2-pretreated animals as compared to animals treated only with phenobarbital. The microsomal cadmium content of animals having received cadmium plus phenobarbital was about 5 times higher than that of animals treated with cadmium alone. The only enzyme activity found to be changed by cadmium in induced microsomes was ethylmorphine-N-deethylation. In phenobarbital-free animals no microsomal changes by cadmium could be detected."}
{"id": "PMID:461992", "title": "Drug metabolism in diabetic isolated perfused rat liver.", "content": "The demethylation of aminopyrine was decreased while the demethylation of p-chloro-N-methylaniline was increased in both alloxan and streptozotocin diabetic isolated perfused male rat livers. Since similar effects of diabetes were observed with traditional microsomal preparations, these observations indicate that the effects of diabetes on drug metabolism observed in isolated microsomal preparations from male rat are an accurate reflection of conditions in the intact organ. These results also clearly indicate that diabetes in the male rat affects at least two different rate-limiting steps in the hepatic drug metabolism process.", "contents": "Drug metabolism in diabetic isolated perfused rat liver. The demethylation of aminopyrine was decreased while the demethylation of p-chloro-N-methylaniline was increased in both alloxan and streptozotocin diabetic isolated perfused male rat livers. Since similar effects of diabetes were observed with traditional microsomal preparations, these observations indicate that the effects of diabetes on drug metabolism observed in isolated microsomal preparations from male rat are an accurate reflection of conditions in the intact organ. These results also clearly indicate that diabetes in the male rat affects at least two different rate-limiting steps in the hepatic drug metabolism process."}
{"id": "PMID:461993", "title": "Hydrolysis of 11-palmitoyloxy-delta 9-tetrahydrocannabinol to 11-hydroxy-delta 9-tetrahydrocannabinol by cholesterol esterase and a lipase.", "content": "11-Palmitoyloxy-delta 9-tetrahydrocannabinol (11-palm-delta 9-THC) has been identified in the rat in our laboratory as a long-retained metabolite of delta 9-tetrahydrocannabinol (delta 9-THC), the active component of marihuana (Leighty, 1973). Although it is not known as yet if 11-palm-delta 9-THC is itself psychoactive it could be hydrolyzed to active 11-hydroxy-delta 9-tetrahydrocannabinol (11-OH-delta 9-THC). Our in vitro studies show that cholesterol esterase and a lipase can hydrolyze 11-palm-delta 9-THC to 11-OH-delta 9-THC.", "contents": "Hydrolysis of 11-palmitoyloxy-delta 9-tetrahydrocannabinol to 11-hydroxy-delta 9-tetrahydrocannabinol by cholesterol esterase and a lipase. 11-Palmitoyloxy-delta 9-tetrahydrocannabinol (11-palm-delta 9-THC) has been identified in the rat in our laboratory as a long-retained metabolite of delta 9-tetrahydrocannabinol (delta 9-THC), the active component of marihuana (Leighty, 1973). Although it is not known as yet if 11-palm-delta 9-THC is itself psychoactive it could be hydrolyzed to active 11-hydroxy-delta 9-tetrahydrocannabinol (11-OH-delta 9-THC). Our in vitro studies show that cholesterol esterase and a lipase can hydrolyze 11-palm-delta 9-THC to 11-OH-delta 9-THC."}
{"id": "PMID:461994", "title": "Identification of benzhydrol as a urinary metabolite of diphenylmethane in the rat.", "content": "Liquid-liquid extraction and preparative thin-layer chromatography were used to isolate 30 micromoles of benzhydrol from the beta-glucuronidase treated 24 hour urine of rats administered 5.3 mmoles of diphenylmethane. The benzhydrol was identified by its melting point, elemental analysis, infrared, nuclear magnetic resonance, and mass spectra.", "contents": "Identification of benzhydrol as a urinary metabolite of diphenylmethane in the rat. Liquid-liquid extraction and preparative thin-layer chromatography were used to isolate 30 micromoles of benzhydrol from the beta-glucuronidase treated 24 hour urine of rats administered 5.3 mmoles of diphenylmethane. The benzhydrol was identified by its melting point, elemental analysis, infrared, nuclear magnetic resonance, and mass spectra."}
{"id": "PMID:461995", "title": "Coenzyme Q10 or alpha-tocopherol reduce the acute toxicity of anthramycin in mice.", "content": "Pretreatment for 4 days with 10 mg/kg coenzyme Q10 (CoQ) or 1 day with 85U alpha-tocopherol (Vit E) produced equal reduction in the acute toxicity of 0.5 mg/kg anthramycin (ANTH). Twenty-seven days after ANTH, 40 percent of control mice survived, those pretreated with CoQ or Vit E displayed 73 and 80 percent survival. A 17.5 mg/kg dose of adriamycin (ADR) produced a percent survival-time curve resembling that produced by 0.5 mg/kg ANTH. At this dose of ADR, however, the protection afforded by CoQ and Vit E was minimal and not statistically significant (p less than 0.05). CoQ or Vit E may be of value at reducing the toxicity of anticancer agents other than the anthracyclines.", "contents": "Coenzyme Q10 or alpha-tocopherol reduce the acute toxicity of anthramycin in mice. Pretreatment for 4 days with 10 mg/kg coenzyme Q10 (CoQ) or 1 day with 85U alpha-tocopherol (Vit E) produced equal reduction in the acute toxicity of 0.5 mg/kg anthramycin (ANTH). Twenty-seven days after ANTH, 40 percent of control mice survived, those pretreated with CoQ or Vit E displayed 73 and 80 percent survival. A 17.5 mg/kg dose of adriamycin (ADR) produced a percent survival-time curve resembling that produced by 0.5 mg/kg ANTH. At this dose of ADR, however, the protection afforded by CoQ and Vit E was minimal and not statistically significant (p less than 0.05). CoQ or Vit E may be of value at reducing the toxicity of anticancer agents other than the anthracyclines."}
{"id": "PMID:461996", "title": "Gas liquid chromatographic studies of [1-14C] palmitate uptake by the perfused guinea-pig heart.", "content": "In addition to the uptake of palmitate by the isolated perfused guinea-pig heart, there was a substantial release of longer chain fatty acids into the perfusate amounting to about 50% of the uptake of palmitate. Both the uptake of palmitate and the release of other fatty acids was diminished in the presence of 95% N2. The specific activity of myocardial palmitate was increased under 95% N2. No significant difference in the distribution of radioactivity between the different myocardial fatty acids occurred between 95% O2 and 95% N2.", "contents": "Gas liquid chromatographic studies of [1-14C] palmitate uptake by the perfused guinea-pig heart. In addition to the uptake of palmitate by the isolated perfused guinea-pig heart, there was a substantial release of longer chain fatty acids into the perfusate amounting to about 50% of the uptake of palmitate. Both the uptake of palmitate and the release of other fatty acids was diminished in the presence of 95% N2. The specific activity of myocardial palmitate was increased under 95% N2. No significant difference in the distribution of radioactivity between the different myocardial fatty acids occurred between 95% O2 and 95% N2."}
{"id": "PMID:461997", "title": "Cannabinoid inhibition of rat luteal cell progesterone synthesis.", "content": "Progesterone production by isolated rat luteal cells was inhibited by exposure to various cannabinoids in concentrations from 3.0--16.0 microM. The available evidence points to the cleavage of cholesterol esters as the site of inhibition.", "contents": "Cannabinoid inhibition of rat luteal cell progesterone synthesis. Progesterone production by isolated rat luteal cells was inhibited by exposure to various cannabinoids in concentrations from 3.0--16.0 microM. The available evidence points to the cleavage of cholesterol esters as the site of inhibition."}
{"id": "PMID:462064", "title": "[Cholecystitis and cholelithiasis induced by lincomycin. Microscopic and ultrastructural studies].", "content": "Gallbladder of 30 golden hamsters was evaluated after a seven days course of lincomycin (60 mg/kg/day). Fifteen animals injected with an equivalent volume of saline and 15 animals non injected at all, were used as controls. Weight, sex, age, and food were similar in all groups. Eight hamsters (23%) treated with lincomycin formed pigmented gallstones.", "contents": "[Cholecystitis and cholelithiasis induced by lincomycin. Microscopic and ultrastructural studies]. Gallbladder of 30 golden hamsters was evaluated after a seven days course of lincomycin (60 mg/kg/day). Fifteen animals injected with an equivalent volume of saline and 15 animals non injected at all, were used as controls. Weight, sex, age, and food were similar in all groups. Eight hamsters (23%) treated with lincomycin formed pigmented gallstones."}
{"id": "PMID:462065", "title": "[Prevention of post appendectomy incision abscess with the use of polyvinilpyrrolidone iodine (prospective study)].", "content": "365 appendicectomy cases performed from 1972 to 1976 were studied prospectively. In 135 control cases the wound was not lavaged. In 102 cases the wound was lavaged with normal saline solution. En 108 cases the wound was lavaged with PVP-I. Wound abscess formation rate in the first group was 28.8%, in the second group was 21.35% and in the third group was 18.5%. Patients operated after 1974 and treated with PVP-I showed a 4.2% abscess formation. Morbility and mortality in appendicitis are best reduced if early diagnosis and surgical treatment are backed by a faultless technic.", "contents": "[Prevention of post appendectomy incision abscess with the use of polyvinilpyrrolidone iodine (prospective study)]. 365 appendicectomy cases performed from 1972 to 1976 were studied prospectively. In 135 control cases the wound was not lavaged. In 102 cases the wound was lavaged with normal saline solution. En 108 cases the wound was lavaged with PVP-I. Wound abscess formation rate in the first group was 28.8%, in the second group was 21.35% and in the third group was 18.5%. Patients operated after 1974 and treated with PVP-I showed a 4.2% abscess formation. Morbility and mortality in appendicitis are best reduced if early diagnosis and surgical treatment are backed by a faultless technic."}
{"id": "PMID:462066", "title": "[Esophageal manometry in patients with sliding hiatal hernia].", "content": "This presents the method to be followed for the valuation of the gastro-esophagic function in patients with sliding hiatal hernia, twenty-one patients with this diagnosis were studied through X-Rays. Besides gastric and esophagic endoscopy, a complete clinical examinations was made to compare the information obtained from the studies, with the direct measurement of the contractions of the esophagus and the lower sphincter through conventional manometric methods. The relationship between the simptoms and the clinical procedures done in the patients were observed as was the usefulness of esophagic manometry to detect not only the direct pressure of the gastro-esophagic sphincter, but also to determine the concurrent motor changes that can occur in patients which show hiatal hernia. The benefit of this studies to decide the therapeutic handling, specially surgical, is discussed.", "contents": "[Esophageal manometry in patients with sliding hiatal hernia]. This presents the method to be followed for the valuation of the gastro-esophagic function in patients with sliding hiatal hernia, twenty-one patients with this diagnosis were studied through X-Rays. Besides gastric and esophagic endoscopy, a complete clinical examinations was made to compare the information obtained from the studies, with the direct measurement of the contractions of the esophagus and the lower sphincter through conventional manometric methods. The relationship between the simptoms and the clinical procedures done in the patients were observed as was the usefulness of esophagic manometry to detect not only the direct pressure of the gastro-esophagic sphincter, but also to determine the concurrent motor changes that can occur in patients which show hiatal hernia. The benefit of this studies to decide the therapeutic handling, specially surgical, is discussed."}
{"id": "PMID:462067", "title": "[Amylase test in the diagnosis of acute pancreatitis].", "content": "The determination of serum and urinary amylase are methods used in the diagnosis of acute pancreatitis, however there are many abdominal problems that can cause hyperamylasemia, in the absence of pancreatic disease, for this reason in 1969 Levitt and col. signaled the possible advantages of amylase/creatinine clearence ratio, in the clinical diagnosis of acute pancreatitis. This test was used in cases with acute pancreatitis, as in other diseases, in correlation with levels in normal subjects, with the objective of evaluating its clinica utility, in relation to the formentioned paragraph. Results concluded that the amylase/creatinine ratio is not of greater diagnostic utility than the determination of urinary amylase, in acute pancreatitis.", "contents": "[Amylase test in the diagnosis of acute pancreatitis]. The determination of serum and urinary amylase are methods used in the diagnosis of acute pancreatitis, however there are many abdominal problems that can cause hyperamylasemia, in the absence of pancreatic disease, for this reason in 1969 Levitt and col. signaled the possible advantages of amylase/creatinine clearence ratio, in the clinical diagnosis of acute pancreatitis. This test was used in cases with acute pancreatitis, as in other diseases, in correlation with levels in normal subjects, with the objective of evaluating its clinica utility, in relation to the formentioned paragraph. Results concluded that the amylase/creatinine ratio is not of greater diagnostic utility than the determination of urinary amylase, in acute pancreatitis."}
{"id": "PMID:462068", "title": "[Eosinophilic gastritis].", "content": "Two case studies of eosinophilic gastritis studied by the Pathology Department of the Hospital Central de San Luis Potosi are presented. Based on these cases, the clinical pathological forms of the entity are analized together with the classification and etiopathology, from which it is considered a late allergic or hipersensitive reaction to foreign proteins. Therefore, it is essentially different from the neoplastic, histiocitic, eosinophilic granuloma of the bone.", "contents": "[Eosinophilic gastritis]. Two case studies of eosinophilic gastritis studied by the Pathology Department of the Hospital Central de San Luis Potosi are presented. Based on these cases, the clinical pathological forms of the entity are analized together with the classification and etiopathology, from which it is considered a late allergic or hipersensitive reaction to foreign proteins. Therefore, it is essentially different from the neoplastic, histiocitic, eosinophilic granuloma of the bone."}
{"id": "PMID:462069", "title": "[Percutaneus cholangiography with the Chiba needle. Results in 220 studies].", "content": "The authors employed this new technique of thin needle percutaneous cholangiography in 220 patients with the following results: 99.3% (146/147) success with previous biliary tract dilatation and 79.4% (53/73) success in patients with normal caliber bile ducts. The overall result of 92.7% (204/228) emphasizes that radiological opacification is the best way to study the icteric patient. Only 1.8% of patients with serious complications is less than in other instrumentation methods. By not requiring specialized equipment and being accessible in the majority of hospitals, study cost is reduced. In conclusion, this new technique of percutaneous cholangiography is preferable for accessibility, efficacy, cost and risk in the study of the icteric patient.", "contents": "[Percutaneus cholangiography with the Chiba needle. Results in 220 studies]. The authors employed this new technique of thin needle percutaneous cholangiography in 220 patients with the following results: 99.3% (146/147) success with previous biliary tract dilatation and 79.4% (53/73) success in patients with normal caliber bile ducts. The overall result of 92.7% (204/228) emphasizes that radiological opacification is the best way to study the icteric patient. Only 1.8% of patients with serious complications is less than in other instrumentation methods. By not requiring specialized equipment and being accessible in the majority of hospitals, study cost is reduced. In conclusion, this new technique of percutaneous cholangiography is preferable for accessibility, efficacy, cost and risk in the study of the icteric patient."}
{"id": "PMID:462072", "title": "[Sensitivity to chemotherapeutic agents of bacteria isolated from generalized infections].", "content": "A study carried out on 206 bacteriemia infections showed that: the following bacterial flora was implicated in the etiology of bacteremia infections: Staphylococcus aureus in 28.6% of cases, Staphylococcus albus in 20.4% Enterobacteriaceae in 25.7% and Pseudomonas in 3.8%; an increase existed in the number of generalized infections caused by opportunists belonging to the Enterobacteriaceae family and Pseudomonas genus. Similarly, an increase in the resistance to the recently introduced antibiotics, Septrin and Gentamycin, was noted. These observation require antibiotic lebel control and, if it is necessary the association of two or more antibiotics in severe infections.", "contents": "[Sensitivity to chemotherapeutic agents of bacteria isolated from generalized infections]. A study carried out on 206 bacteriemia infections showed that: the following bacterial flora was implicated in the etiology of bacteremia infections: Staphylococcus aureus in 28.6% of cases, Staphylococcus albus in 20.4% Enterobacteriaceae in 25.7% and Pseudomonas in 3.8%; an increase existed in the number of generalized infections caused by opportunists belonging to the Enterobacteriaceae family and Pseudomonas genus. Similarly, an increase in the resistance to the recently introduced antibiotics, Septrin and Gentamycin, was noted. These observation require antibiotic lebel control and, if it is necessary the association of two or more antibiotics in severe infections."}
{"id": "PMID:462073", "title": "[Incidence and duration of blood HBs antigen in convalescents after type B viral hepatitis].", "content": "The presence of HBs Ag was determined by contraelectrophoresis in 500 convalescents after hepatitis B, at the moment of discharge and after 1--3 and 6 months. Antigen carriage is prolonged in hepatitis B, 51% of the convalescents being positive on discharge and 10% remaining persistent carriers. This was more frequent in males, young adults and those who had suffered from mild, prolonged forms of the disease. As only 42% of the patients became negative within the first 20 days of hospitalization it became necessary to prolong their stay in hospital and to follow up the patients subsequently in an out-patient unit.", "contents": "[Incidence and duration of blood HBs antigen in convalescents after type B viral hepatitis]. The presence of HBs Ag was determined by contraelectrophoresis in 500 convalescents after hepatitis B, at the moment of discharge and after 1--3 and 6 months. Antigen carriage is prolonged in hepatitis B, 51% of the convalescents being positive on discharge and 10% remaining persistent carriers. This was more frequent in males, young adults and those who had suffered from mild, prolonged forms of the disease. As only 42% of the patients became negative within the first 20 days of hospitalization it became necessary to prolong their stay in hospital and to follow up the patients subsequently in an out-patient unit."}
{"id": "PMID:462070", "title": "[Spontaneous internal biliary fistula. Review of the literature].", "content": "The literature concerning internal biliary fistula is reviewed. Its frequency in autopsy ranges from 0.4 to 5%, and in biliary tract operations from 0.4 to 7%. A general approach has to be established in order to obtain a diagnosis, and an individually oriented treatment provided. Emphasis is placed on certain measures of treatment and complications. The most severe complications are cholangitis (7.5%) and gall stone ileus (2% to 6%).", "contents": "[Spontaneous internal biliary fistula. Review of the literature]. The literature concerning internal biliary fistula is reviewed. Its frequency in autopsy ranges from 0.4 to 5%, and in biliary tract operations from 0.4 to 7%. A general approach has to be established in order to obtain a diagnosis, and an individually oriented treatment provided. Emphasis is placed on certain measures of treatment and complications. The most severe complications are cholangitis (7.5%) and gall stone ileus (2% to 6%)."}
{"id": "PMID:462074", "title": "[Acarids of pathological significance in urine].", "content": "The present paper reports on 7 cases of primary infections, pyelonephritis and pyelocystitis, in which acrids were found in the urine during acute attacks. The cases were detected in the clinical laboratory of a county Hospital. All the 7 patients, ranging in age from 6 to 60 years, exhibited characteristic symptoms of pyelonephritis and pyelocystitis. Numerous acarids were found in the urinary sediment and, in some cases, also their eggs. Some of the acarids were motile, others were encrusted with salts. A female acarid was detected while laying eggs. In 6 patients the acardis were found in the urine only at the first examination and disappeared following the of urinary infection; in the seventh case they were detected at three consecutive examinations performed at 3--6 days interval.", "contents": "[Acarids of pathological significance in urine]. The present paper reports on 7 cases of primary infections, pyelonephritis and pyelocystitis, in which acrids were found in the urine during acute attacks. The cases were detected in the clinical laboratory of a county Hospital. All the 7 patients, ranging in age from 6 to 60 years, exhibited characteristic symptoms of pyelonephritis and pyelocystitis. Numerous acarids were found in the urinary sediment and, in some cases, also their eggs. Some of the acarids were motile, others were encrusted with salts. A female acarid was detected while laying eggs. In 6 patients the acardis were found in the urine only at the first examination and disappeared following the of urinary infection; in the seventh case they were detected at three consecutive examinations performed at 3--6 days interval."}
{"id": "PMID:462071", "title": "[Sensitivity to antibiotics and chemotherapeutic agents in some strains of Salmonella typhi isolated in Romania during the period 1974-1976].", "content": "Investigations were carried out on 781 S. typhi strains isolated from patients and carriers. Testing of the sensitivity to chemotherapeutical agents by diffusiometry and the incorporation technique led to the detection of four resistant strains: 1 strain isolated by coproculture from a carrier since 1958, resistant to A, K, N, Ca and Cf and 3 strains isolated by coproculture, biliculture and uroculture from a carrier since 1974, resistant to A and Ca. The R factors of these strains were transferred in their totality. The resistant strains did not manifest any particular pathogenicity for the laboratory animal, and their immunogenic potential was below that of the current typhoid vaccine. None of the 781S typhi were resistant to Cmx, C, G, T, Co and Po. Consequently, in Romania Chloramphenicol may be used in continuation in the treatment of typhoid fever, and Cotrimoxazol, Gentamycin, Tetracyclin and Ampicillin are and remain reserve substitutes. The possible unexpected appearance of resistant S. typhi strains demand, however, further control of the sensitivity to chemotherapeutics of all the isolated strains.", "contents": "[Sensitivity to antibiotics and chemotherapeutic agents in some strains of Salmonella typhi isolated in Romania during the period 1974-1976]. Investigations were carried out on 781 S. typhi strains isolated from patients and carriers. Testing of the sensitivity to chemotherapeutical agents by diffusiometry and the incorporation technique led to the detection of four resistant strains: 1 strain isolated by coproculture from a carrier since 1958, resistant to A, K, N, Ca and Cf and 3 strains isolated by coproculture, biliculture and uroculture from a carrier since 1974, resistant to A and Ca. The R factors of these strains were transferred in their totality. The resistant strains did not manifest any particular pathogenicity for the laboratory animal, and their immunogenic potential was below that of the current typhoid vaccine. None of the 781S typhi were resistant to Cmx, C, G, T, Co and Po. Consequently, in Romania Chloramphenicol may be used in continuation in the treatment of typhoid fever, and Cotrimoxazol, Gentamycin, Tetracyclin and Ampicillin are and remain reserve substitutes. The possible unexpected appearance of resistant S. typhi strains demand, however, further control of the sensitivity to chemotherapeutics of all the isolated strains."}
{"id": "PMID:462087", "title": "[The value of sonography in evaluation of unilateral nonvisualized kidney (author's transl)].", "content": "In a comparative study with 35 patients, sonography, angiography and radiorenograms are investigated on their validity for evaluation of nonvisualized kidneys. In our group of patients, angiography showed an accuracy quota of 97% (34/35) and sonography showed an accuracy quota of 88% (31/35). In 12 patients, the radiorenograms unequivocally show functional loss with the nephrectomy type. Sonography should be used first as a simple noninvasive procedure. It is independent of the remaining renal function and indicates the size of the organ. For hydronephrosis sonography cannot clarify the cause of the urinary stasis in some cases; for this purpose, further examinations are indicated (retrograde pyelography, antegrade pyelography).", "contents": "[The value of sonography in evaluation of unilateral nonvisualized kidney (author's transl)]. In a comparative study with 35 patients, sonography, angiography and radiorenograms are investigated on their validity for evaluation of nonvisualized kidneys. In our group of patients, angiography showed an accuracy quota of 97% (34/35) and sonography showed an accuracy quota of 88% (31/35). In 12 patients, the radiorenograms unequivocally show functional loss with the nephrectomy type. Sonography should be used first as a simple noninvasive procedure. It is independent of the remaining renal function and indicates the size of the organ. For hydronephrosis sonography cannot clarify the cause of the urinary stasis in some cases; for this purpose, further examinations are indicated (retrograde pyelography, antegrade pyelography)."}
{"id": "PMID:462088", "title": "[Radiological diagnosis of the \u00f6sophagus with special attention to the \u00f6sophagogastric transition of the gastro-\u00f6sophageal reflux, reflux \u00f6sophagitis, hiatal hernias and \u00f6sophageal stenoses (author's transl)].", "content": "The inferior high-pressure zone acts like a functional sphincter. This is formed in the absence of a muscle correlate, via a regional predominance of the sphincter tone caused by inhibition of activity of the longitudinal muscles through the membrane phreno-oesophagea in the region of the vestibulum gastro-oesophageale. Pressure variations in the vestibulum caused by respiration, are governed by a \"rein mechanism\". Swallowing produces a brief reflectory breakdown of the inferior high-pressure zone in the longitudinal muscles of the \u00f6sophagus via myogenic ventricular stimulus condution. Gastro-\u00f6sophageal reflux can be regularly identified by means of suitable x-ray examination technique. Since, however, it is not possible to arrive at a quantitative and qualitative assessment of the reflux via roentgenology, the aim of radiological examination in case of clinical suspicion of reflux is to determine a) the factors favouring reflux, ie hiatal hernia, gastric and duodenal ulcer, stenosis of the pylorus, etc, b) the sequelae of a pathological reflux, eg reflux stenosis, reflux ulcer, brachy\u00f6sophagus etc, as well as c) to assess the self-cleaning function of the \u00f6sophagus. It is possible to definitely identify hiatal hernia by radiology with a well-aimed examination technique, through the intrathoracal displacement of parts of the stomach, of Schatzki's ring, and the superhiatal displacement of the entire vestibulum gastro-oesophageale. It is also regularly possible to identify by serial radiography an organic \u00f6sophagus stenosis, using large quantities of barium swallow, although pathogenetic interpretation of the x-ray film may be difficulat. Functional stenosis can be defined by pharmacoradiography and manometry. In clinically suspected reflux \u00f6sophagitis, endoscopy and histology will supply more conclusive findings in respect of mucosal assessment than x-ray examination.", "contents": "[Radiological diagnosis of the \u00f6sophagus with special attention to the \u00f6sophagogastric transition of the gastro-\u00f6sophageal reflux, reflux \u00f6sophagitis, hiatal hernias and \u00f6sophageal stenoses (author's transl)]. The inferior high-pressure zone acts like a functional sphincter. This is formed in the absence of a muscle correlate, via a regional predominance of the sphincter tone caused by inhibition of activity of the longitudinal muscles through the membrane phreno-oesophagea in the region of the vestibulum gastro-oesophageale. Pressure variations in the vestibulum caused by respiration, are governed by a \"rein mechanism\". Swallowing produces a brief reflectory breakdown of the inferior high-pressure zone in the longitudinal muscles of the \u00f6sophagus via myogenic ventricular stimulus condution. Gastro-\u00f6sophageal reflux can be regularly identified by means of suitable x-ray examination technique. Since, however, it is not possible to arrive at a quantitative and qualitative assessment of the reflux via roentgenology, the aim of radiological examination in case of clinical suspicion of reflux is to determine a) the factors favouring reflux, ie hiatal hernia, gastric and duodenal ulcer, stenosis of the pylorus, etc, b) the sequelae of a pathological reflux, eg reflux stenosis, reflux ulcer, brachy\u00f6sophagus etc, as well as c) to assess the self-cleaning function of the \u00f6sophagus. It is possible to definitely identify hiatal hernia by radiology with a well-aimed examination technique, through the intrathoracal displacement of parts of the stomach, of Schatzki's ring, and the superhiatal displacement of the entire vestibulum gastro-oesophageale. It is also regularly possible to identify by serial radiography an organic \u00f6sophagus stenosis, using large quantities of barium swallow, although pathogenetic interpretation of the x-ray film may be difficulat. Functional stenosis can be defined by pharmacoradiography and manometry. In clinically suspected reflux \u00f6sophagitis, endoscopy and histology will supply more conclusive findings in respect of mucosal assessment than x-ray examination."}
{"id": "PMID:462089", "title": "[Diagnosis of subpulmonary effusion in children (author's transl)].", "content": "If an elevation of the right or left base of the lung is found on a chest x-ray film, differential diagnosis will have to consider the follwoing possibilities: 1. Elevation of the diaphragm, 2. Subpulmonary effusion and 3. Hernia of abdominal organs through the diaphragm. Suspicion of a subpulmonary effusion is prompted by the following x-ray findings: 1. Flattening of the costophrenic angle and elevation of the base of the lung. 2. Increased distance between \"magenblase\" (stomach bubble) and base of the lung. 3. Elevation of the lower margins of the lung, the apex being in the region of the lateral hemithorax. 4. Translucency of the margins of the diaphragm below the elevated base of the lung. 5. Absence of vascular shadoes behind the diaphragm on a wellexposed x-ray film. If one or several of these symptoms is seen, proof should be obtained by provoking issuing of the effusion by placing the patient in supine position or in lateral position on the affected side, and by trying to achieve visualization of the resulting condition.", "contents": "[Diagnosis of subpulmonary effusion in children (author's transl)]. If an elevation of the right or left base of the lung is found on a chest x-ray film, differential diagnosis will have to consider the follwoing possibilities: 1. Elevation of the diaphragm, 2. Subpulmonary effusion and 3. Hernia of abdominal organs through the diaphragm. Suspicion of a subpulmonary effusion is prompted by the following x-ray findings: 1. Flattening of the costophrenic angle and elevation of the base of the lung. 2. Increased distance between \"magenblase\" (stomach bubble) and base of the lung. 3. Elevation of the lower margins of the lung, the apex being in the region of the lateral hemithorax. 4. Translucency of the margins of the diaphragm below the elevated base of the lung. 5. Absence of vascular shadoes behind the diaphragm on a wellexposed x-ray film. If one or several of these symptoms is seen, proof should be obtained by provoking issuing of the effusion by placing the patient in supine position or in lateral position on the affected side, and by trying to achieve visualization of the resulting condition."}
{"id": "PMID:462090", "title": "[Diagnosis of combined malformation of lung and pulmonary vessels (author's transl)].", "content": "Diagnosis of combined malformation of lung and pulmonary vessels are presented in a case of one lobe lung on the right (Agenesis of two lobes and hypoplasia of the presenting lobe). The importance of radioisotope methods in diagnosis of lung hypoplasia is stressed. Pathogenesis and period of teratogenic termination is discussed.", "contents": "[Diagnosis of combined malformation of lung and pulmonary vessels (author's transl)]. Diagnosis of combined malformation of lung and pulmonary vessels are presented in a case of one lobe lung on the right (Agenesis of two lobes and hypoplasia of the presenting lobe). The importance of radioisotope methods in diagnosis of lung hypoplasia is stressed. Pathogenesis and period of teratogenic termination is discussed."}
{"id": "PMID:462091", "title": "[Hypertrophy of Hoffa's fat pad - an arthrographic diagnosis? (author's transl)].", "content": "The wide spectrum of conditions, ranging from normal variants over early posttraumatic swelling to Hoffa's classical syndrome with hypertrophy of the fat pad can be demonstrated in double-contrast arthrography with high reliability, demonstrated after review of 1566 arthrographies in comparison with results of surgery and histology. Following post-mortem studies in 50 knee joints statements are made about normal size and shape, physiological variants and pathological enlargement of Hoffa's fat pad in arthrography. Since post-traumatic changes of menisci, cartilage and Hoffa body often cannot be differentiated by clinical examination, double-contrast arthrography should be performed routinely before surgery, since this technique provides the surgeon with a maximum of preoperative information about the internal structures of the knee joint and may modify the planned surgical procedure considerably.", "contents": "[Hypertrophy of Hoffa's fat pad - an arthrographic diagnosis? (author's transl)]. The wide spectrum of conditions, ranging from normal variants over early posttraumatic swelling to Hoffa's classical syndrome with hypertrophy of the fat pad can be demonstrated in double-contrast arthrography with high reliability, demonstrated after review of 1566 arthrographies in comparison with results of surgery and histology. Following post-mortem studies in 50 knee joints statements are made about normal size and shape, physiological variants and pathological enlargement of Hoffa's fat pad in arthrography. Since post-traumatic changes of menisci, cartilage and Hoffa body often cannot be differentiated by clinical examination, double-contrast arthrography should be performed routinely before surgery, since this technique provides the surgeon with a maximum of preoperative information about the internal structures of the knee joint and may modify the planned surgical procedure considerably."}
{"id": "PMID:462092", "title": "[On the technique of examination in ERCP, EPT with stone extraction and PTC from the radiologist's viewpoint (author's transl)].", "content": "Based on the empirical data of more than 2500 examinations we report on the technique of the endoscopic retrograde cholangio- and pancreaticography (ERCP), the endoscopic papillotomy (EPT) with calculus extraction and also the percutaneus transhepatic cholangiography (PTC) from the radiologic point of view. The essential tasks of the radiologist are the clarifying of the position of the probe, the quick diagnosis and the making of valid x-ray photographs. For this purpose the knowledge about an optimal technique of examination is the prerequisite. By this the experienced radiologist is an important partner of the gastroenterologist. He helps to avoid complications for the patient.", "contents": "[On the technique of examination in ERCP, EPT with stone extraction and PTC from the radiologist's viewpoint (author's transl)]. Based on the empirical data of more than 2500 examinations we report on the technique of the endoscopic retrograde cholangio- and pancreaticography (ERCP), the endoscopic papillotomy (EPT) with calculus extraction and also the percutaneus transhepatic cholangiography (PTC) from the radiologic point of view. The essential tasks of the radiologist are the clarifying of the position of the probe, the quick diagnosis and the making of valid x-ray photographs. For this purpose the knowledge about an optimal technique of examination is the prerequisite. By this the experienced radiologist is an important partner of the gastroenterologist. He helps to avoid complications for the patient."}
{"id": "PMID:462093", "title": "[CT scanning in cystic liver changes (author's transl)].", "content": "Computer tomography is outstandingly suitable for detecting cystic processes in the liver. The genesis of such cysts can vary widely, so that there are bound to be differences in the clinical relevance of the findings. This is discussed on the basis of 20 case histories of cystic changes of the liver observed during one and a half years. For the purpose of discussion of the value of CT scanning, comparisons with sonographic and angiographic examinations were carried out.", "contents": "[CT scanning in cystic liver changes (author's transl)]. Computer tomography is outstandingly suitable for detecting cystic processes in the liver. The genesis of such cysts can vary widely, so that there are bound to be differences in the clinical relevance of the findings. This is discussed on the basis of 20 case histories of cystic changes of the liver observed during one and a half years. For the purpose of discussion of the value of CT scanning, comparisons with sonographic and angiographic examinations were carried out."}
{"id": "PMID:462094", "title": "[Angiographic examinations in hypertension. Vascular morphology, flow rate and kidney size (author's transl)].", "content": "1. The paper reports on examinations relating to vascular morphology, flow rate and kidney size in patients suffering from arterial hypertension. 2. The results and findings differ clearly from those of the total group of patients and a control group of healthy volunteers. 3. The results and observations are discussed. 4. Renal angiography can give more information than commonly assumed. Hence it should be employed more frequently than before for the purpose of clarifying diagnosis in patients with arterial hypertension.", "contents": "[Angiographic examinations in hypertension. Vascular morphology, flow rate and kidney size (author's transl)]. 1. The paper reports on examinations relating to vascular morphology, flow rate and kidney size in patients suffering from arterial hypertension. 2. The results and findings differ clearly from those of the total group of patients and a control group of healthy volunteers. 3. The results and observations are discussed. 4. Renal angiography can give more information than commonly assumed. Hence it should be employed more frequently than before for the purpose of clarifying diagnosis in patients with arterial hypertension."}
{"id": "PMID:462095", "title": "[Cinefluorographic studies in children with previous esophageal atresia repair (author's transl)].", "content": "Seven children with previous esophageal Atresia repair underwent cinefluorographic studies of the swalling act. They all showed disordered motor function in the esophageal segment below the Anastomosis with segmental contractions and lack of primary Peristalsis. Nevertheless we found organized propulsive waves below the Anastomosis too, which leaded to an esophageal emptying also in the supine position.", "contents": "[Cinefluorographic studies in children with previous esophageal atresia repair (author's transl)]. Seven children with previous esophageal Atresia repair underwent cinefluorographic studies of the swalling act. They all showed disordered motor function in the esophageal segment below the Anastomosis with segmental contractions and lack of primary Peristalsis. Nevertheless we found organized propulsive waves below the Anastomosis too, which leaded to an esophageal emptying also in the supine position."}
{"id": "PMID:462096", "title": "[Roentgen examination after oesophageal surgery (author's transl)].", "content": "Sutureinsufficiency has to be sought postoperatively. Later, tumors, passage disturbances and reflux must be looked for. Antirefluxsurgery with fundoplication may lead to a roentgenmorphology with tumoraspect. After transoesophageal sclerosis of varices passage disturbances and perforations may be seen. After perforations by endoscopic procedures the oesophageal roentgen examination with water-soluble substances completes the clinical surveillance.", "contents": "[Roentgen examination after oesophageal surgery (author's transl)]. Sutureinsufficiency has to be sought postoperatively. Later, tumors, passage disturbances and reflux must be looked for. Antirefluxsurgery with fundoplication may lead to a roentgenmorphology with tumoraspect. After transoesophageal sclerosis of varices passage disturbances and perforations may be seen. After perforations by endoscopic procedures the oesophageal roentgen examination with water-soluble substances completes the clinical surveillance."}
{"id": "PMID:462097", "title": "[Spontaneous Ahlb\u00e4ck's knee joint osteonecrosis].", "content": "Among the group of genuine osteonecroses there ranks the spontaneous osteonecrosis of the knee described for the first time by Ahlb\u00e4ck, Bauer and Bohne in 1968. Characteristic of it is a subchondral defect on the medial condylus of the femur, which may occur in old persons, chiefly women. Supported by six illustrative cases in own patients clinical experience, radiology, diagnosis and differential diagnosis are presented and discussed.", "contents": "[Spontaneous Ahlb\u00e4ck's knee joint osteonecrosis]. Among the group of genuine osteonecroses there ranks the spontaneous osteonecrosis of the knee described for the first time by Ahlb\u00e4ck, Bauer and Bohne in 1968. Characteristic of it is a subchondral defect on the medial condylus of the femur, which may occur in old persons, chiefly women. Supported by six illustrative cases in own patients clinical experience, radiology, diagnosis and differential diagnosis are presented and discussed."}
{"id": "PMID:462098", "title": "[X-ray diagnosis of retropatellar diseases (author's transl)].", "content": "The article reports on a comprehensive, stepwise diagnosis in diseases of the knee joints. This includes a description of the indication, the technique of taking x-ray films, and x-ray findings, as well as arthrography of the femoropatellar joint in retropatellar diseases such as chondropathia patellae, osteochondrosis dissecans, traumas of the knee joints and arthrosis deformans.", "contents": "[X-ray diagnosis of retropatellar diseases (author's transl)]. The article reports on a comprehensive, stepwise diagnosis in diseases of the knee joints. This includes a description of the indication, the technique of taking x-ray films, and x-ray findings, as well as arthrography of the femoropatellar joint in retropatellar diseases such as chondropathia patellae, osteochondrosis dissecans, traumas of the knee joints and arthrosis deformans."}
{"id": "PMID:462107", "title": "Serum and pituitary prolactin variations under the influence of pyridoxine and pimozide in the male rat.", "content": "Prolactin was measured in the serum and hypophyses of the male rat after six days of i.p. administration of pyridoxine 5 mg (group B6), after a single dose of pimozide 2.5 mg/kg body (group PMZ), six days of pyridoxine and one dose of pimozide (group B6- PMZ) and a single dose of tartaric acid (controls). Both pyridoxine and pimozide increased the serum prolactin: 59.06 +/- 16.23 ng/ml and 68.45 +/- 10.78 ng/ml respectively (X +/- DS) by comparison to controls: 33.12 +/- 11.16 ng/ml, their effect being cumulative (83.75 +/- 13.86 ng/ml). However, the effect of pyridoxine and pimozide at the central level is different, pyridoxine producing an increase of the pituitary concentration of prolactin, while pimozide depleted the stored prolactin. Hypothetical mechanisms of pyridoxine action at the pituitary level are discussed.", "contents": "Serum and pituitary prolactin variations under the influence of pyridoxine and pimozide in the male rat. Prolactin was measured in the serum and hypophyses of the male rat after six days of i.p. administration of pyridoxine 5 mg (group B6), after a single dose of pimozide 2.5 mg/kg body (group PMZ), six days of pyridoxine and one dose of pimozide (group B6- PMZ) and a single dose of tartaric acid (controls). Both pyridoxine and pimozide increased the serum prolactin: 59.06 +/- 16.23 ng/ml and 68.45 +/- 10.78 ng/ml respectively (X +/- DS) by comparison to controls: 33.12 +/- 11.16 ng/ml, their effect being cumulative (83.75 +/- 13.86 ng/ml). However, the effect of pyridoxine and pimozide at the central level is different, pyridoxine producing an increase of the pituitary concentration of prolactin, while pimozide depleted the stored prolactin. Hypothetical mechanisms of pyridoxine action at the pituitary level are discussed."}
{"id": "PMID:462106", "title": "Decrease of testosterone, cholesterol and ascorbic acid in the rat testis after administration of pineal polypeptides.", "content": "Administration of 2 ml/day/animal, of melatonin-free pineal extract to the rat for three days induced a significant decrease in the testicular tissue concentration of testosterone, cholesterol and ascorbic acid. A dose of 1 microgram melatonin/day/animal given for 3 days did not alter these biochemical elements implied in testicular steroidogenesis.", "contents": "Decrease of testosterone, cholesterol and ascorbic acid in the rat testis after administration of pineal polypeptides. Administration of 2 ml/day/animal, of melatonin-free pineal extract to the rat for three days induced a significant decrease in the testicular tissue concentration of testosterone, cholesterol and ascorbic acid. A dose of 1 microgram melatonin/day/animal given for 3 days did not alter these biochemical elements implied in testicular steroidogenesis."}
{"id": "PMID:462105", "title": "Changes in the bioelectric activity of the lateral septal nucleus, dorsal hippocampus and medial habenular nucleus after administration of the pineal peptide extract \"crinofizin\".", "content": "New Zealand rabbits with pairs of electrodes implanted chronically in the RFM, dorsal hippocampus, LSN and HM were tasted for electroencephalographic changes in the dorsal hippocampus, lateral septal nucleus and medial habenular nuclei in response to electric stimulation of the mesencephalic reticular formation and after slow i.v. and i.m. injection with the pineal peptidic extract \"Crinofizin\". Stimulation of RFM with a rectangular current of variable tension (1.5--4 volts) induced synusoidal waves on the EEG of the dorsal hippocampus, LSN and HM with a frequency of 4 to over 8 c/s persisting throughout the electric stimulation. Intramuscular administration of 10 ml pineal extract in doses of 2 ml every 48 hrs led to changes in the EEG traces in that the mean frequency of the synusoidal waves decreased from 3.8 c/s to 1.4 c/s whereas the mean amplitude of the waves was almost threefold the mean amplitude of the background traces.", "contents": "Changes in the bioelectric activity of the lateral septal nucleus, dorsal hippocampus and medial habenular nucleus after administration of the pineal peptide extract \"crinofizin\". New Zealand rabbits with pairs of electrodes implanted chronically in the RFM, dorsal hippocampus, LSN and HM were tasted for electroencephalographic changes in the dorsal hippocampus, lateral septal nucleus and medial habenular nuclei in response to electric stimulation of the mesencephalic reticular formation and after slow i.v. and i.m. injection with the pineal peptidic extract \"Crinofizin\". Stimulation of RFM with a rectangular current of variable tension (1.5--4 volts) induced synusoidal waves on the EEG of the dorsal hippocampus, LSN and HM with a frequency of 4 to over 8 c/s persisting throughout the electric stimulation. Intramuscular administration of 10 ml pineal extract in doses of 2 ml every 48 hrs led to changes in the EEG traces in that the mean frequency of the synusoidal waves decreased from 3.8 c/s to 1.4 c/s whereas the mean amplitude of the waves was almost threefold the mean amplitude of the background traces."}
{"id": "PMID:462109", "title": "Transsexualism; new observations.", "content": "Ten cases of transsexualism have been studied. All the patients present gonadal lesions, modifications of the morphotype and small hormonologic anomalies. It is supposed that the gonadal lesions are frequently associated with this psycho-behavioural disorder. Thus transsexualism may be considered a trouble of the whole process of post gonadal sexualization.", "contents": "Transsexualism; new observations. Ten cases of transsexualism have been studied. All the patients present gonadal lesions, modifications of the morphotype and small hormonologic anomalies. It is supposed that the gonadal lesions are frequently associated with this psycho-behavioural disorder. Thus transsexualism may be considered a trouble of the whole process of post gonadal sexualization."}
{"id": "PMID:462110", "title": "Reactivity to calcitonin after ovariectomy.", "content": "The hypocalcemic response to calcitonin injected in female rats disappeared after their castration. The substitution treatment with estrogens does no allow reestablishment of the hypocalcemic effect obtained in intact animals, a fact that lays stress on the role of the whole ovarian secretion in maintaining a normal response to calcitonin. The response of castrated animals to calcitonin calls attention to the conditional role of estrogens in validating the osteoprotecting effect of calcitonin.", "contents": "Reactivity to calcitonin after ovariectomy. The hypocalcemic response to calcitonin injected in female rats disappeared after their castration. The substitution treatment with estrogens does no allow reestablishment of the hypocalcemic effect obtained in intact animals, a fact that lays stress on the role of the whole ovarian secretion in maintaining a normal response to calcitonin. The response of castrated animals to calcitonin calls attention to the conditional role of estrogens in validating the osteoprotecting effect of calcitonin."}
{"id": "PMID:462112", "title": "Variations of catecholamines in mixed shock. Therapeutic implications. Experimental and clinical investigations.", "content": "The variations of catecholamines, cortisol and aldosterone in mixed shock (traumatic, hemorrhagic and septic) were studied experimentally and clinically. In the stage of collapse adrenaline decreased by 81.4% and noradrenaline by 62.6% as compared with the preoperative values. These decreases were found to be directly proportional to the decrease of arterial blood pressure, pulse amplitude and diuresis as well as to the slowing down or even stop of microcirculation. The value of plasma cortisol increased in the stage of collapse by 98.3% and that of aldosterone by 66.27% as compared with the preoperative values. Treatment with a pharmacodynamic sympatheticolytic-vasopressor-adrenocortical mixture is recommanded when the blood pressure remains below 50--60 mm Hg for 1--2 hrs and when the treatment for the correction of hypovolemia with fluids and sympatheticolytics has failed. Clinically, this treatment gave good results in 34 of the 58 cases studied (58.6%).", "contents": "Variations of catecholamines in mixed shock. Therapeutic implications. Experimental and clinical investigations. The variations of catecholamines, cortisol and aldosterone in mixed shock (traumatic, hemorrhagic and septic) were studied experimentally and clinically. In the stage of collapse adrenaline decreased by 81.4% and noradrenaline by 62.6% as compared with the preoperative values. These decreases were found to be directly proportional to the decrease of arterial blood pressure, pulse amplitude and diuresis as well as to the slowing down or even stop of microcirculation. The value of plasma cortisol increased in the stage of collapse by 98.3% and that of aldosterone by 66.27% as compared with the preoperative values. Treatment with a pharmacodynamic sympatheticolytic-vasopressor-adrenocortical mixture is recommanded when the blood pressure remains below 50--60 mm Hg for 1--2 hrs and when the treatment for the correction of hypovolemia with fluids and sympatheticolytics has failed. Clinically, this treatment gave good results in 34 of the 58 cases studied (58.6%)."}
{"id": "PMID:462123", "title": "Effect of normal human serum on the binding of specific antibodies and platelet-unrelated immune complexes to human platelets.", "content": "Radiolabelled staphylococcal protein A was used to quantitate the binding of IgG on stored human platelets from human sera containing specific antibodies reactive with platelets and rabbit serum containing immune complexes (IC). Normal human serum (NHS) inhibited the binding of IC onto platelets and to various extents also the binding of specific antibodies. The attachment of inhibitors to platelets seemed to be reversible. The considerable difference in the inhibitory capacities of IgG-deficient sera and monomeric IgG indicates that IgG is the major inhibitory component of NHS. The binding of IgG from NHS onto platelets evidently hampers the detection of weak platelet antibodies even with the most sensitive tests. Purified Clq, known to modify the reactions of IC with fresh platelets did not alter the binding of IC onto stored platelets. A monoclonal, antiglobulin-active rheumatoid factor of IgM class displayed only moderate inhibition. Therefore, the application of RF or Clq for the differentiation of the binding induced by IC or antibodies is not useful in this assay system. The heterogeneity of immunologic receptors of platelets provides an explanation of the inhibitory inefficiency of Clq.", "contents": "Effect of normal human serum on the binding of specific antibodies and platelet-unrelated immune complexes to human platelets. Radiolabelled staphylococcal protein A was used to quantitate the binding of IgG on stored human platelets from human sera containing specific antibodies reactive with platelets and rabbit serum containing immune complexes (IC). Normal human serum (NHS) inhibited the binding of IC onto platelets and to various extents also the binding of specific antibodies. The attachment of inhibitors to platelets seemed to be reversible. The considerable difference in the inhibitory capacities of IgG-deficient sera and monomeric IgG indicates that IgG is the major inhibitory component of NHS. The binding of IgG from NHS onto platelets evidently hampers the detection of weak platelet antibodies even with the most sensitive tests. Purified Clq, known to modify the reactions of IC with fresh platelets did not alter the binding of IC onto stored platelets. A monoclonal, antiglobulin-active rheumatoid factor of IgM class displayed only moderate inhibition. Therefore, the application of RF or Clq for the differentiation of the binding induced by IC or antibodies is not useful in this assay system. The heterogeneity of immunologic receptors of platelets provides an explanation of the inhibitory inefficiency of Clq."}
{"id": "PMID:462124", "title": "Detection of Fc receptor-bearing lymphocytes by using IgG-coated latex particles.", "content": "Latex particles coated with human IgG can be used to detect lymphocytes capable of binding to the Fc portion of IgG. Optimal conditions are described. The method is as sensitive as techniques using antibody-coated erythrocytes, is easy to perform, and has good reproducibility. A small proportion of T cells and the majority of B cells can be shown to carry receptors for Fc (IgG).", "contents": "Detection of Fc receptor-bearing lymphocytes by using IgG-coated latex particles. Latex particles coated with human IgG can be used to detect lymphocytes capable of binding to the Fc portion of IgG. Optimal conditions are described. The method is as sensitive as techniques using antibody-coated erythrocytes, is easy to perform, and has good reproducibility. A small proportion of T cells and the majority of B cells can be shown to carry receptors for Fc (IgG)."}
{"id": "PMID:462125", "title": "Clonotype patterns of antibodies released by single lymph nodes.", "content": "Experimental primary, secondary and tertiary stimulation with streptococcal vaccine of isolated lymph nodes in vivo in the sheep model induces largely persistence of anti-group polysaccharide antibody clonotype patterns with the rare occurrence of additional clonotypes demonstrable after secondary stimulation persisting during the tertiary stimulus. It is not clear whether these additional clonotypes are the products of mutants or whether they pre-existed and were demonstrable only after a secondary stimulus, because of threshold concentrations required for identification. Further, isolated contralateral popliteal and prescapular lymph nodes of individual sheep share completely overlapping clonotype patterns during experimental primary anti-polysaccharide antibody responses indicating an identical repertoire of specific clonotypes under this condition of responsiveness.", "contents": "Clonotype patterns of antibodies released by single lymph nodes. Experimental primary, secondary and tertiary stimulation with streptococcal vaccine of isolated lymph nodes in vivo in the sheep model induces largely persistence of anti-group polysaccharide antibody clonotype patterns with the rare occurrence of additional clonotypes demonstrable after secondary stimulation persisting during the tertiary stimulus. It is not clear whether these additional clonotypes are the products of mutants or whether they pre-existed and were demonstrable only after a secondary stimulus, because of threshold concentrations required for identification. Further, isolated contralateral popliteal and prescapular lymph nodes of individual sheep share completely overlapping clonotype patterns during experimental primary anti-polysaccharide antibody responses indicating an identical repertoire of specific clonotypes under this condition of responsiveness."}
{"id": "PMID:462126", "title": "Degradation of thymidine to thymine by rheumatoid arthritis synovial tissue eluate.", "content": "A study was made of the effect of rheumatoid synovial tissue eluate on phytohaemagglutinin (PHA) stimulation of peripheral blood lymphocytes. It was found that rheumatoid synovial tissue eluate caused marked inhibition of 3H-thymidine incorporation by lymphocytes stimulated with PHA. Synovial tissue from traumatic joints had no effect. The inhibitor was a heat-sensitive and nondialysable substance. The inhibitory effect of PHA stimulation was diminished by thymine. In thin-layer chromatography it showed the capacity to convert thymidine to thymine. Because thymine is not incorporated by DNA-synthesizing cells, this report explains why negative results are obtained when lymphocytes are stimulated by rheumatoid synovial tissue using 3H-thymidine incorporation as an indicator of lymphocyte activation.", "contents": "Degradation of thymidine to thymine by rheumatoid arthritis synovial tissue eluate. A study was made of the effect of rheumatoid synovial tissue eluate on phytohaemagglutinin (PHA) stimulation of peripheral blood lymphocytes. It was found that rheumatoid synovial tissue eluate caused marked inhibition of 3H-thymidine incorporation by lymphocytes stimulated with PHA. Synovial tissue from traumatic joints had no effect. The inhibitor was a heat-sensitive and nondialysable substance. The inhibitory effect of PHA stimulation was diminished by thymine. In thin-layer chromatography it showed the capacity to convert thymidine to thymine. Because thymine is not incorporated by DNA-synthesizing cells, this report explains why negative results are obtained when lymphocytes are stimulated by rheumatoid synovial tissue using 3H-thymidine incorporation as an indicator of lymphocyte activation."}
{"id": "PMID:462127", "title": "Hearing impairment in meningococcal meningitis.", "content": "Hearing affection as a sequel of meningococcal meningitis and its relation to age, sex, severity and duration of disease was studied in Cairo, Egypt during the period December 1966--December 1973. The total incidence of impaired hearing in the 775 cases was 5.8%. This incidence was higher in the younger age groups, females, severe cases of meningitis and in patients who received specific therapy shortly after the onset of signs and symptoms of the disease. The aetiology of hearing impairment in meningitis was reviewed.", "contents": "Hearing impairment in meningococcal meningitis. Hearing affection as a sequel of meningococcal meningitis and its relation to age, sex, severity and duration of disease was studied in Cairo, Egypt during the period December 1966--December 1973. The total incidence of impaired hearing in the 775 cases was 5.8%. This incidence was higher in the younger age groups, females, severe cases of meningitis and in patients who received specific therapy shortly after the onset of signs and symptoms of the disease. The aetiology of hearing impairment in meningitis was reviewed."}
{"id": "PMID:462128", "title": "Cefamandole nafate: an evaluation of antibacterial activity, serum levels, clinical effect, and incidence of side reactions in 58 patients.", "content": "35 males and 23 females with skin and soft tissue infection, lower respiratory tract infection, urinary tract infection or septicemia with known etiology were treated with cefamandole nafate. The patients were to a large extent (30%) alcoholics and/or drug abusers. Cefamandole was given intravenously as 4 daily doses of 1 g in 52 cases and of 2 g in 6 cases for 8 to 16 (mean 10) days. The effect was considered to be good in 40 patients (70%). Adverse reactions, mostly slight and transient, were seen in 22 patients (38%). Peak serum levels varied from 26 to 82 (mean 50) micrograms/ml after 1 g doses and from 68 to 100 micrograms/ml after 2 g. Previous statements of a better in vitro activity of cefamandole than of older cephalosporins against some gram-negative bacilli were corroborated.", "contents": "Cefamandole nafate: an evaluation of antibacterial activity, serum levels, clinical effect, and incidence of side reactions in 58 patients. 35 males and 23 females with skin and soft tissue infection, lower respiratory tract infection, urinary tract infection or septicemia with known etiology were treated with cefamandole nafate. The patients were to a large extent (30%) alcoholics and/or drug abusers. Cefamandole was given intravenously as 4 daily doses of 1 g in 52 cases and of 2 g in 6 cases for 8 to 16 (mean 10) days. The effect was considered to be good in 40 patients (70%). Adverse reactions, mostly slight and transient, were seen in 22 patients (38%). Peak serum levels varied from 26 to 82 (mean 50) micrograms/ml after 1 g doses and from 68 to 100 micrograms/ml after 2 g. Previous statements of a better in vitro activity of cefamandole than of older cephalosporins against some gram-negative bacilli were corroborated."}
{"id": "PMID:462129", "title": "Tetracyclines and host defense mechanisms: interference with leukocyte chemotaxis.", "content": "The influence of 2 etracycline preparations, lymecycline and doxycycline, on leukocyte chemotaxis, was investigated in vitro and in vivo. Doxycycline in high concentrations caused a severe depression of both spontaneous and induced migration, an effect which was more pronounced than that caused by similar concentration of lymecycline. Corresponding results were obtained both in experiments on healthy volunteers given recommended dosages of the antibiotics, and in patients treated with doxycycline for postoperative infections.", "contents": "Tetracyclines and host defense mechanisms: interference with leukocyte chemotaxis. The influence of 2 etracycline preparations, lymecycline and doxycycline, on leukocyte chemotaxis, was investigated in vitro and in vivo. Doxycycline in high concentrations caused a severe depression of both spontaneous and induced migration, an effect which was more pronounced than that caused by similar concentration of lymecycline. Corresponding results were obtained both in experiments on healthy volunteers given recommended dosages of the antibiotics, and in patients treated with doxycycline for postoperative infections."}
{"id": "PMID:462130", "title": "Clindamycin excretion in the pancreatic fluid of dogs.", "content": "Clindamycin phosphate in dosage of 15 mg/kg was administered intravenously to dogs. Serum and pancreatic juice clindamycin concentrations were assayed. Pancreatic juice clindamycin concentrations varied between 1.32--5.5 micrograms/ml. Clindamycin was still detectable in the pancreatic juice 4 1/2 h after its administration. Clindamycin, in combination with other antimicrobials, may be potentially effective in the prophylaxis and therapy of some of the infectious complications associated with pancreatitis.", "contents": "Clindamycin excretion in the pancreatic fluid of dogs. Clindamycin phosphate in dosage of 15 mg/kg was administered intravenously to dogs. Serum and pancreatic juice clindamycin concentrations were assayed. Pancreatic juice clindamycin concentrations varied between 1.32--5.5 micrograms/ml. Clindamycin was still detectable in the pancreatic juice 4 1/2 h after its administration. Clindamycin, in combination with other antimicrobials, may be potentially effective in the prophylaxis and therapy of some of the infectious complications associated with pancreatitis."}
{"id": "PMID:462131", "title": "Treatment of experimental Naegleria meningoencephalitis with a combination of amphotericin B and rifamycin.", "content": "The treatment of primary amoebic meningoencephalitis was examined using a mouse model. Rifamycin by itself was ineffective. However, a synergistic effect was observed when used in combination with amphotericin B. This finding may prove useful in the treatment of patients with primary amoebic meningoencephalitis, for which satisfactory treatment is currently unavailable.", "contents": "Treatment of experimental Naegleria meningoencephalitis with a combination of amphotericin B and rifamycin. The treatment of primary amoebic meningoencephalitis was examined using a mouse model. Rifamycin by itself was ineffective. However, a synergistic effect was observed when used in combination with amphotericin B. This finding may prove useful in the treatment of patients with primary amoebic meningoencephalitis, for which satisfactory treatment is currently unavailable."}
{"id": "PMID:462132", "title": "Risk of infection among seafarers: a prospective seroepidemiological survey of the crew of a passenger cruiser.", "content": "Serial blood specimens for various serological examinations were taken from the crew of a passenger ship which sailed on winter cruises. The study covered 3 successive seasons totalling about 150 years of observation. The figures on the rates of infection were compared with data available from the general population. The study revealed no clear evidence of an increased infection risk among the crew.", "contents": "Risk of infection among seafarers: a prospective seroepidemiological survey of the crew of a passenger cruiser. Serial blood specimens for various serological examinations were taken from the crew of a passenger ship which sailed on winter cruises. The study covered 3 successive seasons totalling about 150 years of observation. The figures on the rates of infection were compared with data available from the general population. The study revealed no clear evidence of an increased infection risk among the crew."}
{"id": "PMID:462133", "title": "The prevalence of toxoplasma antibodies among 11,736 pregnant women in Norway.", "content": "A serologic screening for toxoplasma antibodies by dye test (DT) and IgG indirect fluorescent antibody test (IgG-IFAT) has been carried out among 10729 pregnant women in the Oslo area and 1 007 pregnant women from the county of M\u00f8re and Romsdal situated on the west coast of Norway. The frequency of toxoplasma antibodies was found to be higher in women living in rural areas and a higher prevalence was observed among women living in the western districts of Oslo than among women from the eastern districts of the city. The mean frequency of DT antibodies was found to be 12.5% in the Oslo area and 13.3% in M\u00f8re and Romsdal, whereas the yearly incidence of acquired infections in the two areas was found to be 5 and 2.3%, respectively. The predicted incidence of congenital toxoplasmosis in the Oslo area, and presumably also in the whole country, would represent 2% of all pregnancies. No serologic differences were found when women with prior histories of either sporadic or habitual abortions were compared to a total group of multigravidae among the Oslo women.", "contents": "The prevalence of toxoplasma antibodies among 11,736 pregnant women in Norway. A serologic screening for toxoplasma antibodies by dye test (DT) and IgG indirect fluorescent antibody test (IgG-IFAT) has been carried out among 10729 pregnant women in the Oslo area and 1 007 pregnant women from the county of M\u00f8re and Romsdal situated on the west coast of Norway. The frequency of toxoplasma antibodies was found to be higher in women living in rural areas and a higher prevalence was observed among women living in the western districts of Oslo than among women from the eastern districts of the city. The mean frequency of DT antibodies was found to be 12.5% in the Oslo area and 13.3% in M\u00f8re and Romsdal, whereas the yearly incidence of acquired infections in the two areas was found to be 5 and 2.3%, respectively. The predicted incidence of congenital toxoplasmosis in the Oslo area, and presumably also in the whole country, would represent 2% of all pregnancies. No serologic differences were found when women with prior histories of either sporadic or habitual abortions were compared to a total group of multigravidae among the Oslo women."}
{"id": "PMID:462134", "title": "Passive--active immunization in a neonate treated with repeated doses of high-titred hepatitis B immune globulin.", "content": "A newborn infant, born to a HBsAg-positive drug addict with HBeAg and DNA-polymerase activity in serum, was treated with repeated very high doses of hepatitis B immune globulin (HBIg). The mother turned HBsAg-negative about 8 months after deliver and the HBIg prophylaxis was then discontinued. A check-up of the infant about a year later revealed normal liver function tests but significantly increased levels of antibodies against HBcAg and HBsAg indicating a post subclinical hepatitis B infection, apparently without harm to the infant.", "contents": "Passive--active immunization in a neonate treated with repeated doses of high-titred hepatitis B immune globulin. A newborn infant, born to a HBsAg-positive drug addict with HBeAg and DNA-polymerase activity in serum, was treated with repeated very high doses of hepatitis B immune globulin (HBIg). The mother turned HBsAg-negative about 8 months after deliver and the HBIg prophylaxis was then discontinued. A check-up of the infant about a year later revealed normal liver function tests but significantly increased levels of antibodies against HBcAg and HBsAg indicating a post subclinical hepatitis B infection, apparently without harm to the infant."}
{"id": "PMID:462135", "title": "Recurrent pneumonia and encephalitis due to Mycoplasma pneumoniae.", "content": "Recurrent Mycoplasma pneumoniae encephalitis in a young man is reported. The patient appeared not to be immunodeficient and despite the presence of a focal inflammatory brain lesion with a predominance of polymorphonuclear cells no direct evidence of inent in M. pneumoniae respiratory tract infection still is unknown the case strongly indicates that certain individuals are somehow predisposed to such complications. The case also illustrates that CNS complications may occur even during a mild mycoplasma respiratory tract infection and that the radiological findings can mimic cerebral haemorrhage or abscess necessitating neurosurgical exploration.", "contents": "Recurrent pneumonia and encephalitis due to Mycoplasma pneumoniae. Recurrent Mycoplasma pneumoniae encephalitis in a young man is reported. The patient appeared not to be immunodeficient and despite the presence of a focal inflammatory brain lesion with a predominance of polymorphonuclear cells no direct evidence of inent in M. pneumoniae respiratory tract infection still is unknown the case strongly indicates that certain individuals are somehow predisposed to such complications. The case also illustrates that CNS complications may occur even during a mild mycoplasma respiratory tract infection and that the radiological findings can mimic cerebral haemorrhage or abscess necessitating neurosurgical exploration."}
{"id": "PMID:462136", "title": "Prophylaxis of neurosurgical infections by improved preoperative disinfection of the scalp.", "content": "The heads of neurosurgical patients were subject to 2 scrubbings with hexachlorophene detergent, performed in the ward prior to operation. After this single change of routines the frequency of wound infections after craniotomy dropped 4-fold, from 2% to 0.5%. The result indicates that the scalp of the patient is the major source of the staphylococci that cause neurosurgical infections.", "contents": "Prophylaxis of neurosurgical infections by improved preoperative disinfection of the scalp. The heads of neurosurgical patients were subject to 2 scrubbings with hexachlorophene detergent, performed in the ward prior to operation. After this single change of routines the frequency of wound infections after craniotomy dropped 4-fold, from 2% to 0.5%. The result indicates that the scalp of the patient is the major source of the staphylococci that cause neurosurgical infections."}
{"id": "PMID:462137", "title": "Measles virus specific precipitins in sera from patients with chronic active hepatitis.", "content": "Measles virus specific antibodies in sera from 29 patients with chronic active hepatitis have been examined using standard serological tests and immunoelectrophoretic techniques. Significantly raised CF antibody titres against measles virus antigen were demonstrated in 50% of the sera. The major immunoprecipitate obtained by immunoelectrophoresis was shown to contain aggregates of measles virus nucleocapsids. Antibodies against the haemagglutinin antigen of the virus envelope were also raised and were demonstrated by line immunoelectrophoresis.", "contents": "Measles virus specific precipitins in sera from patients with chronic active hepatitis. Measles virus specific antibodies in sera from 29 patients with chronic active hepatitis have been examined using standard serological tests and immunoelectrophoretic techniques. Significantly raised CF antibody titres against measles virus antigen were demonstrated in 50% of the sera. The major immunoprecipitate obtained by immunoelectrophoresis was shown to contain aggregates of measles virus nucleocapsids. Antibodies against the haemagglutinin antigen of the virus envelope were also raised and were demonstrated by line immunoelectrophoresis."}
{"id": "PMID:462138", "title": "Evaluations of dangerousness.", "content": "The Supreme Court decided in O'Connor v. Donaldson that mentally ill people who were not dangerous and who could survive safely in the community could not be kept in a mental hospital against their will. In 1976, a project evaluated the legality of the commitments of 107 patients in a State mental hospital. The review was conducted by three panels, each composed of a law student, a resident psychiatrist, and a social worker. Evaluations of a patient's dangerousness were made on the basis of information from the patient's hospital records, an interview with the patient's primary therapist at the hospital, and interviews with the patient. Analysis of project recommendations revealed that commitment decisions were more related to the clinical outlook of the evaluators than to the patient's behavior.", "contents": "Evaluations of dangerousness. The Supreme Court decided in O'Connor v. Donaldson that mentally ill people who were not dangerous and who could survive safely in the community could not be kept in a mental hospital against their will. In 1976, a project evaluated the legality of the commitments of 107 patients in a State mental hospital. The review was conducted by three panels, each composed of a law student, a resident psychiatrist, and a social worker. Evaluations of a patient's dangerousness were made on the basis of information from the patient's hospital records, an interview with the patient's primary therapist at the hospital, and interviews with the patient. Analysis of project recommendations revealed that commitment decisions were more related to the clinical outlook of the evaluators than to the patient's behavior."}
{"id": "PMID:462139", "title": "Environment and inheritance: opposing forces?", "content": "High risk of schizophrenia requires high genetic predisposition to develop schizophrenia, plus an environmental trigger. A schizophrenic family environment is commonly believed to represent this trigger. The hypothesis is presented here that, on the contrary, a high predisposition to schizophrenia in significant others protects against overt illness. The trigger may be the predominance around the index individual of significant others with low predisposition to develop schizophrenia.", "contents": "Environment and inheritance: opposing forces? High risk of schizophrenia requires high genetic predisposition to develop schizophrenia, plus an environmental trigger. A schizophrenic family environment is commonly believed to represent this trigger. The hypothesis is presented here that, on the contrary, a high predisposition to schizophrenia in significant others protects against overt illness. The trigger may be the predominance around the index individual of significant others with low predisposition to develop schizophrenia."}
{"id": "PMID:462140", "title": "Rejecting the patient: preliminary validation of a self-report scale.", "content": "The authors describe an easily administered scale being developed to assess the rejecting feelings of family respondents toward former mental patients who return to live with their families. Based upon a sample of 133 discharged mental patients and family members, the scale demonstrates a moderately high reliability (alpha = .78) and test-retest correlation (r = .72). Data are presented for evaluating the construct validity of the scale.", "contents": "Rejecting the patient: preliminary validation of a self-report scale. The authors describe an easily administered scale being developed to assess the rejecting feelings of family respondents toward former mental patients who return to live with their families. Based upon a sample of 133 discharged mental patients and family members, the scale demonstrates a moderately high reliability (alpha = .78) and test-retest correlation (r = .72). Data are presented for evaluating the construct validity of the scale."}
{"id": "PMID:462141", "title": "The mental hospital revisited: a proposal.", "content": "Mental hospital treatment stresses an overcaring, humanistic approach that tends to foster a passive role dependence of patients on institutions and people. The author proposes a treatment concept of an \"active, self-participatory involvement\" by patients. This approach allows patients to experience an active role by participation in various tasks and assumption of responsibilities in running the ward. Staff gradually delegates decision-making processes, implementation of plans, and even a certain administrative authority to patients.", "contents": "The mental hospital revisited: a proposal. Mental hospital treatment stresses an overcaring, humanistic approach that tends to foster a passive role dependence of patients on institutions and people. The author proposes a treatment concept of an \"active, self-participatory involvement\" by patients. This approach allows patients to experience an active role by participation in various tasks and assumption of responsibilities in running the ward. Staff gradually delegates decision-making processes, implementation of plans, and even a certain administrative authority to patients."}
{"id": "PMID:462142", "title": "Taximetric strategies applied to psychiatric classification.", "content": "This article discusses limitations inherent in using unilevel behavioral descriptors as the basis for the construction of psychiatric classifications. As an alternative we consider the advantages of the polythetic classification strategy as suggested by Sokal and Sneath (1963) in their description of a \"numerical taxonomic\" approach. They argue for the development of naturally occurring, empirically defined classes rather than categories based on single domains of behaviors such as psychiatric symptoms. Typically the research into a topic such as schizophrenia starts with a behaviorally defined class of individuals who show schizophrenic symptoms, contrasted with a nonschizophrenic control group. These groups are then tested for aberrations in other systems of functioning (blood, neurophysiological, hormonal, attentional functioning, and the like). The approach offered here requires a classificatory strategy that treats data from all levels of functioning as equivalent in forming the classes of individuals rather than as secondary to the defining symptomatic attributes. Particular emphasis is given to the choice of measures with sufficiently broad and sensitive features to find natural classes. A set of standards is given for the selection of a test battery that might be used in a polythetic approach to the investigation of schizophrenia. Finally, the utility and limits of statistical clustering techniques for handling multilevel data banks are discussed.", "contents": "Taximetric strategies applied to psychiatric classification. This article discusses limitations inherent in using unilevel behavioral descriptors as the basis for the construction of psychiatric classifications. As an alternative we consider the advantages of the polythetic classification strategy as suggested by Sokal and Sneath (1963) in their description of a \"numerical taxonomic\" approach. They argue for the development of naturally occurring, empirically defined classes rather than categories based on single domains of behaviors such as psychiatric symptoms. Typically the research into a topic such as schizophrenia starts with a behaviorally defined class of individuals who show schizophrenic symptoms, contrasted with a nonschizophrenic control group. These groups are then tested for aberrations in other systems of functioning (blood, neurophysiological, hormonal, attentional functioning, and the like). The approach offered here requires a classificatory strategy that treats data from all levels of functioning as equivalent in forming the classes of individuals rather than as secondary to the defining symptomatic attributes. Particular emphasis is given to the choice of measures with sufficiently broad and sensitive features to find natural classes. A set of standards is given for the selection of a test battery that might be used in a polythetic approach to the investigation of schizophrenia. Finally, the utility and limits of statistical clustering techniques for handling multilevel data banks are discussed."}
{"id": "PMID:462143", "title": "Longitudinal social competence and adult psychiatric symptoms at first hospitalization.", "content": "Patterns of psychiatric symptoms of 141 patients at first hospital admission were correlated with social competence, as measured in childhood from school records and in adulthood by the Index of Social Competence, which is based on hospital records. Results confirmed the hypothesis that low social competence is associated with the more disintegrative symptoms of withdrawal, thought disorder, and antisocial acting out, but this conclusion held only when the measure of social competence was based upon adult premorbid behavior. A longitudinal perspective on social competence did not improve upon the symptomatic discrimination based on adult cross-sectional assessment alone, except that a cluster of schizoid symptoms (apathy, flat affect, hallucinations, resentfulness, and verbal hostility) was significantly associated with a longitudinal measure of social competence, though not with either cross-sectional measure by itself. Positive symptoms (delusions, hallucinations, and other florid processes) appeared not to be part of a longstanding, longitudinal process, but the negative symptoms included in the withdrawal cluster showed some association with childhood behavior.", "contents": "Longitudinal social competence and adult psychiatric symptoms at first hospitalization. Patterns of psychiatric symptoms of 141 patients at first hospital admission were correlated with social competence, as measured in childhood from school records and in adulthood by the Index of Social Competence, which is based on hospital records. Results confirmed the hypothesis that low social competence is associated with the more disintegrative symptoms of withdrawal, thought disorder, and antisocial acting out, but this conclusion held only when the measure of social competence was based upon adult premorbid behavior. A longitudinal perspective on social competence did not improve upon the symptomatic discrimination based on adult cross-sectional assessment alone, except that a cluster of schizoid symptoms (apathy, flat affect, hallucinations, resentfulness, and verbal hostility) was significantly associated with a longitudinal measure of social competence, though not with either cross-sectional measure by itself. Positive symptoms (delusions, hallucinations, and other florid processes) appeared not to be part of a longstanding, longitudinal process, but the negative symptoms included in the withdrawal cluster showed some association with childhood behavior."}
{"id": "PMID:462144", "title": "Natural healing processes of the mind: I. Acute schizophrenic disorganization.", "content": "It is proposed that there are three fundamental adaptive systems that have developed in the course of evolution. One of these, learning, is the only one that is widely recognized. The other two consist of regulation of the energetic aspect of stimulation, and integration of the data of experience into an organized conceptual system. Corresponding to each of the three basic adaptive systems are three basic types of disorder, namely maladaptive learning, which is by far the most common source of behavioral problems; overstimulation, as in the traumatic neurosis; and collapse of the individual's conceptual integrative system, as in acute schizophrenic disorganization. Just as relearning is a natural adaptive process for correcting problems produced by learning, there are inherent processes for correcting the disorders produced by overstimulation and by a maladaptive integrative system. The present article is concerned with the natural process for correcting a poorly organized conceptual system. A second article (Epstein 1978) will be devoted to the natural process of coping with the energic aspects of stimulation. An acute disorganizational state can provide an opportunity for a new and more effective conceptual system to develop. As a desperate remedy, it is often unsuccessful. Nevertheless, an orderly process can be discerned in such states that can enhance the likelihood that new, and dissociated old, material will be assimilated into a new organization. If all goes well, the new organization will be more resilient than the old one; it will be better able to accomplish the functions of an implicit theory of reality, which are to integrate the data of experience, to maintain a favorable pleasure-pain balance, and to maintain self-esteem.", "contents": "Natural healing processes of the mind: I. Acute schizophrenic disorganization. It is proposed that there are three fundamental adaptive systems that have developed in the course of evolution. One of these, learning, is the only one that is widely recognized. The other two consist of regulation of the energetic aspect of stimulation, and integration of the data of experience into an organized conceptual system. Corresponding to each of the three basic adaptive systems are three basic types of disorder, namely maladaptive learning, which is by far the most common source of behavioral problems; overstimulation, as in the traumatic neurosis; and collapse of the individual's conceptual integrative system, as in acute schizophrenic disorganization. Just as relearning is a natural adaptive process for correcting problems produced by learning, there are inherent processes for correcting the disorders produced by overstimulation and by a maladaptive integrative system. The present article is concerned with the natural process for correcting a poorly organized conceptual system. A second article (Epstein 1978) will be devoted to the natural process of coping with the energic aspects of stimulation. An acute disorganizational state can provide an opportunity for a new and more effective conceptual system to develop. As a desperate remedy, it is often unsuccessful. Nevertheless, an orderly process can be discerned in such states that can enhance the likelihood that new, and dissociated old, material will be assimilated into a new organization. If all goes well, the new organization will be more resilient than the old one; it will be better able to accomplish the functions of an implicit theory of reality, which are to integrate the data of experience, to maintain a favorable pleasure-pain balance, and to maintain self-esteem."}
{"id": "PMID:462145", "title": "Language, reality, and schizophrenia.", "content": "In a series of articles, D.V. Forrest (1965, 1969, 1973, 1976) has attacked the contention of many cognitive psychologists that schizophrenic behavior is best signified by deviations in language and aberrancies of thought. Forrest maintains that although schizophrenics do indeed diverge from conventional standards of language and thought, such divergence is not unusual or disordered; that the schizophrenic's world is intentionally coded and is not in any way nonsensical or entropic. In defense of his position, Forrest makes three claims: (1) that all language is inherently metaphorical; (2) that the activities of the schizophrenic are akin to those of the artist; and (3) that reality is nominal and indeterminate (therefore no description of \"schizophrenia\" has ontological status or refers specifically to a psychopathological type). It will be the purpose of this report to show that the above claims cannot be upheld, that overwhelming difficulties pervade Forrest's position, and that said difficulties arise from a misunderstanding of language.", "contents": "Language, reality, and schizophrenia. In a series of articles, D.V. Forrest (1965, 1969, 1973, 1976) has attacked the contention of many cognitive psychologists that schizophrenic behavior is best signified by deviations in language and aberrancies of thought. Forrest maintains that although schizophrenics do indeed diverge from conventional standards of language and thought, such divergence is not unusual or disordered; that the schizophrenic's world is intentionally coded and is not in any way nonsensical or entropic. In defense of his position, Forrest makes three claims: (1) that all language is inherently metaphorical; (2) that the activities of the schizophrenic are akin to those of the artist; and (3) that reality is nominal and indeterminate (therefore no description of \"schizophrenia\" has ontological status or refers specifically to a psychopathological type). It will be the purpose of this report to show that the above claims cannot be upheld, that overwhelming difficulties pervade Forrest's position, and that said difficulties arise from a misunderstanding of language."}
{"id": "PMID:462146", "title": "Early mobilization of patients with unstable fractures of the thoracic and lumbar spine. A 2-year follow-up study.", "content": "During the acute immobilization period 18 patients, with unstable fractures of the spine together with, in most cases, a spinal cord lesion, caudally of T2, were tilted in bed or on a tilting table. Twelve of these patients began the tilting already in the second week after the injury and reached +30 degrees on the 15th day, +50 degrees on the 37th day and +90 degrees on the 65th day following the injury. All patients were kept under observation at regular intervals up to 2 years. A comparison between similar patient groups, treated strictly conservatively, and the present patient groups did not show any differences either in respect to deformities in the spinal column, expected reduction in the spreading of the spinal cord lesions or in the subjective symptoms of the patients. In the hope of reducing the complications induced by protracted immobilization, the authors recommend that patients with unstable fractures of the spinal column, caudally of T2, with or without spinal cord lesion, begin a specified tilting program already in the second week following the injury.", "contents": "Early mobilization of patients with unstable fractures of the thoracic and lumbar spine. A 2-year follow-up study. During the acute immobilization period 18 patients, with unstable fractures of the spine together with, in most cases, a spinal cord lesion, caudally of T2, were tilted in bed or on a tilting table. Twelve of these patients began the tilting already in the second week after the injury and reached +30 degrees on the 15th day, +50 degrees on the 37th day and +90 degrees on the 65th day following the injury. All patients were kept under observation at regular intervals up to 2 years. A comparison between similar patient groups, treated strictly conservatively, and the present patient groups did not show any differences either in respect to deformities in the spinal column, expected reduction in the spreading of the spinal cord lesions or in the subjective symptoms of the patients. In the hope of reducing the complications induced by protracted immobilization, the authors recommend that patients with unstable fractures of the spinal column, caudally of T2, with or without spinal cord lesion, begin a specified tilting program already in the second week following the injury."}
{"id": "PMID:462147", "title": "Circulatory strain in everyday life of paraplegics.", "content": "Circulatory strain encountered in everyday life was low, taxing 15-24% of HRR. Similar results (Th8-Th12) was estimated from their heart rates (fH) recorded continuously by portable tape recorders for up to 48 h. The degree of strain was expressed in per cent of the individual's heart rate reserve (HRR), i.e. of the span between resting and maximal fH. The average strain of everyday life was low, taxing 15-24% of HRR. Similar results were obtained in a rehabilitated patient (subj. 5, Th12) at the hospital. fH higher than 50% of HRR which could be expected to have a training effect on the circulatory system was recoreded only when ambulating with crutches, driving wheelchair uphill, playing basketball, or during specific training (arm cranking in subj. 6 (Th2) who attended a post-rehabilitation course). The results confirm that daily life activities with no additional training are not intense enough to maintain the circulatory and physical fitness of rehabilitated paraplegics.", "contents": "Circulatory strain in everyday life of paraplegics. Circulatory strain encountered in everyday life was low, taxing 15-24% of HRR. Similar results (Th8-Th12) was estimated from their heart rates (fH) recorded continuously by portable tape recorders for up to 48 h. The degree of strain was expressed in per cent of the individual's heart rate reserve (HRR), i.e. of the span between resting and maximal fH. The average strain of everyday life was low, taxing 15-24% of HRR. Similar results were obtained in a rehabilitated patient (subj. 5, Th12) at the hospital. fH higher than 50% of HRR which could be expected to have a training effect on the circulatory system was recoreded only when ambulating with crutches, driving wheelchair uphill, playing basketball, or during specific training (arm cranking in subj. 6 (Th2) who attended a post-rehabilitation course). The results confirm that daily life activities with no additional training are not intense enough to maintain the circulatory and physical fitness of rehabilitated paraplegics."}
{"id": "PMID:462148", "title": "Force-velocity relationships in the human quadriceps muscles.", "content": "Isokinetic contraction were performed with the knee extensor muscles in 15 young male soccer players. The measurements were obtained by a modified isokinetic device (Orthotron). In the range of motion the angular velocity was constant from 90 degrees to 20 degrees of knee flexion. The angular velocity could be varied from 30 to 360 degrees/s. The overall variation of the pre-set speed and the peak torque, as estimated from duplicate trials through the whole velocity range, averaged 4% and 5%, respectively. The peak torque decreased linearly with increasing angular velocity in a semilogarithmic scale. An estimate of the peak torque-velocity relationship in an experimental subject was obtained from the slope and the fibre composition in the lateral portion of the quandriceps muscle in this group of subjects especially trained in fast movements. It was concluded that the present applied isokinetic device gives the opportunities to measure force-velocity characteristics in the intact quadriceps muscle of man.", "contents": "Force-velocity relationships in the human quadriceps muscles. Isokinetic contraction were performed with the knee extensor muscles in 15 young male soccer players. The measurements were obtained by a modified isokinetic device (Orthotron). In the range of motion the angular velocity was constant from 90 degrees to 20 degrees of knee flexion. The angular velocity could be varied from 30 to 360 degrees/s. The overall variation of the pre-set speed and the peak torque, as estimated from duplicate trials through the whole velocity range, averaged 4% and 5%, respectively. The peak torque decreased linearly with increasing angular velocity in a semilogarithmic scale. An estimate of the peak torque-velocity relationship in an experimental subject was obtained from the slope and the fibre composition in the lateral portion of the quandriceps muscle in this group of subjects especially trained in fast movements. It was concluded that the present applied isokinetic device gives the opportunities to measure force-velocity characteristics in the intact quadriceps muscle of man."}
{"id": "PMID:462149", "title": "[Surgery of bronchial neoplasms].", "content": "Surgical resection is the most efficient therapy for lung cancer. Preoperative investigation should determine histology, local invasion and distant dissemination. Tumor-host relation (immunocompetence and tumor burden) is essential for long-term results. Relatively crude anatomical staging does not take biological parameters into account, and any case with lympho-glandular involvement (N1) should not be considered as stage I carcinoma. 520 cases treated by pulmonary resection are reviewed and common denominators for long-term survivors determined. Stage I epidermoid carcinoma treated by lobectomy or left pneumonectomy with a short history (survey cases) are the ideal candidates for surgical resection. The importance of early diagnosis by routine screening is stressed.", "contents": "[Surgery of bronchial neoplasms]. Surgical resection is the most efficient therapy for lung cancer. Preoperative investigation should determine histology, local invasion and distant dissemination. Tumor-host relation (immunocompetence and tumor burden) is essential for long-term results. Relatively crude anatomical staging does not take biological parameters into account, and any case with lympho-glandular involvement (N1) should not be considered as stage I carcinoma. 520 cases treated by pulmonary resection are reviewed and common denominators for long-term survivors determined. Stage I epidermoid carcinoma treated by lobectomy or left pneumonectomy with a short history (survey cases) are the ideal candidates for surgical resection. The importance of early diagnosis by routine screening is stressed."}
{"id": "PMID:462150", "title": "[Socioeconomic aspects of lung cancer].", "content": "The socio-economic aspects of lung cancer are briefly considered, first, with regard to the causes conditioning its occurrence and clinical progress. Tobacco smoking, occupational exposure and air pollution are (in decreasing order of importance) the main determinants of lung cancer occurrence, which differs as between social classes. Social class also appears to be related to clinical progress of the disease. The economic consequences of lung cancer may be tentatively estimated, for a country like Switzerland, at 40 million Swiss francs per year, a sum which can be greatly reduced if a sustained collective effort at prevention is implemented.", "contents": "[Socioeconomic aspects of lung cancer]. The socio-economic aspects of lung cancer are briefly considered, first, with regard to the causes conditioning its occurrence and clinical progress. Tobacco smoking, occupational exposure and air pollution are (in decreasing order of importance) the main determinants of lung cancer occurrence, which differs as between social classes. Social class also appears to be related to clinical progress of the disease. The economic consequences of lung cancer may be tentatively estimated, for a country like Switzerland, at 40 million Swiss francs per year, a sum which can be greatly reduced if a sustained collective effort at prevention is implemented."}
{"id": "PMID:462151", "title": "[Bronchial cytology--value of various methods for the diagnosis of primary lung neoplasms. A computation of the materials sent in to the cytological laboratory of the Institute of Pathology, Kantonspital, St. Gallen].", "content": "During the period 1973-1977 some 6100 sputum cytologies from 3000 patients were performed. All patients had suspected primary bronchogenic carcinoma. Sputum cytology was diagnostic in 547 patients (18%). A synopsis of all patients with proven lung cancer and at least one primary sputum cytology shows that this method was diagnostic in 80%. Fiberoptic bronchoscopy with its associated procedures (biopsies, brushings, washings) was the most frequently performed endoscopic method at our Department of Medicine. Fiberbronchoscopy used in combination with cytologic brushings was diagnostic in 83% of the proven cancers. Formerly, bronchial washings and bronchial secretions were obtained by means of rigid bronchoscope. This material yielded the lowest percentage (51%) of all cytological methods used at the Cantonal Hospital. The indications for use of the different diagnostic methods mentioned above and important factors which yield a high percentage of positive cytologies are discussed.", "contents": "[Bronchial cytology--value of various methods for the diagnosis of primary lung neoplasms. A computation of the materials sent in to the cytological laboratory of the Institute of Pathology, Kantonspital, St. Gallen]. During the period 1973-1977 some 6100 sputum cytologies from 3000 patients were performed. All patients had suspected primary bronchogenic carcinoma. Sputum cytology was diagnostic in 547 patients (18%). A synopsis of all patients with proven lung cancer and at least one primary sputum cytology shows that this method was diagnostic in 80%. Fiberoptic bronchoscopy with its associated procedures (biopsies, brushings, washings) was the most frequently performed endoscopic method at our Department of Medicine. Fiberbronchoscopy used in combination with cytologic brushings was diagnostic in 83% of the proven cancers. Formerly, bronchial washings and bronchial secretions were obtained by means of rigid bronchoscope. This material yielded the lowest percentage (51%) of all cytological methods used at the Cantonal Hospital. The indications for use of the different diagnostic methods mentioned above and important factors which yield a high percentage of positive cytologies are discussed."}
{"id": "PMID:462152", "title": "Application of tumor marker analysis to patients with lung cancer.", "content": "Measurement of Clq-BA and CEA levels in patients with lung cancer may provide additional information about their clinical status discriminating between disease free patients and those with residual disease. Post-operative determination of Clq-BA and CEA levels may assist in defining patients which have poor prognosis. Preliminary evidence suggests that Clq-BA measurement may provide additional prognostic information in a small group of patients with normal CEA values. Finally, the presence of elevated Clq-BA in tumor bearing patients is of fundamental importance in the biology of cancer as it suggests that there are some tumor associated antigens which provoke the production of antibody in the host with the resultant formation of circulating immune complexes. The definition of the nature of the antigen in these immune complexes awaits further study.", "contents": "Application of tumor marker analysis to patients with lung cancer. Measurement of Clq-BA and CEA levels in patients with lung cancer may provide additional information about their clinical status discriminating between disease free patients and those with residual disease. Post-operative determination of Clq-BA and CEA levels may assist in defining patients which have poor prognosis. Preliminary evidence suggests that Clq-BA measurement may provide additional prognostic information in a small group of patients with normal CEA values. Finally, the presence of elevated Clq-BA in tumor bearing patients is of fundamental importance in the biology of cancer as it suggests that there are some tumor associated antigens which provoke the production of antibody in the host with the resultant formation of circulating immune complexes. The definition of the nature of the antigen in these immune complexes awaits further study."}
{"id": "PMID:462153", "title": "[Presurgical determination of lung function in patients with bronchogenic carcinoma].", "content": "90 out of 202 lung cancer patients hospitalized for preoperative investigations were scheduled for radical surgery. 15 patients were not operated on because of insufficient lung function data. The operation was performed in 75 patients. The complication rate was 11% and mortality 8%. 7 out of 8 patients who subsequently developed severe complications had fulfilled the criteria for lung resection only after intensive antibronchitic treatment. Nearly all the survivors showed the predicted preoperative minimal lung function values after the operation. The limit of 1000 ml for postoperative FEV1 is justified, since patients with these or larger functional reserves nearly all had a favourable early and late postoperative course, and also because lung function values continuously deteriorate in chronic obstructive lung disease.", "contents": "[Presurgical determination of lung function in patients with bronchogenic carcinoma]. 90 out of 202 lung cancer patients hospitalized for preoperative investigations were scheduled for radical surgery. 15 patients were not operated on because of insufficient lung function data. The operation was performed in 75 patients. The complication rate was 11% and mortality 8%. 7 out of 8 patients who subsequently developed severe complications had fulfilled the criteria for lung resection only after intensive antibronchitic treatment. Nearly all the survivors showed the predicted preoperative minimal lung function values after the operation. The limit of 1000 ml for postoperative FEV1 is justified, since patients with these or larger functional reserves nearly all had a favourable early and late postoperative course, and also because lung function values continuously deteriorate in chronic obstructive lung disease."}
{"id": "PMID:462154", "title": "[Bronchoscopic diagnosis by means of rigid or flexible optics].", "content": "In order to expand the diagnostic potentialities of bronchoscopy and decrease its risk to the patient, the author has combined rigid-tube endoscopy with the flexible fibroscopy over the last 4 years. Rigid optical systems (angulated and straight) afford far better optical quality, whereas the flexible optic oment of the lower lobe. General anesthesia through the bronchoscopy tube lowers patient stress and discomfort and, in contrast to fiberbronchoscopy alone, allows assisted breathing of the high-risk patient.", "contents": "[Bronchoscopic diagnosis by means of rigid or flexible optics]. In order to expand the diagnostic potentialities of bronchoscopy and decrease its risk to the patient, the author has combined rigid-tube endoscopy with the flexible fibroscopy over the last 4 years. Rigid optical systems (angulated and straight) afford far better optical quality, whereas the flexible optic oment of the lower lobe. General anesthesia through the bronchoscopy tube lowers patient stress and discomfort and, in contrast to fiberbronchoscopy alone, allows assisted breathing of the high-risk patient."}
{"id": "PMID:462155", "title": "[The place of total aerodigestive upper endoscopy in oncology].", "content": "The epidemiology of upper digestive and bronchogenic carcinoma shows a large overlap between the high risk groups. Thus, a candidate for bronchogenic carcinoma is also a possible candidate for upper digestive cancer. Furthermore, the high frequency of multiple carcinoma in the upper aero-digestive tract, the esophagus and the tracheo-bronchial tree warrants a pan-endoscopic (buccal-pharyngo-esophagoscopy and laryngo-tracheo-bronchoscopy) approach to the problem. Using this type of endoscopy, 16 multiple carcinomas have so far been simultaneously discovered among 200 pan-endoscopies.", "contents": "[The place of total aerodigestive upper endoscopy in oncology]. The epidemiology of upper digestive and bronchogenic carcinoma shows a large overlap between the high risk groups. Thus, a candidate for bronchogenic carcinoma is also a possible candidate for upper digestive cancer. Furthermore, the high frequency of multiple carcinoma in the upper aero-digestive tract, the esophagus and the tracheo-bronchial tree warrants a pan-endoscopic (buccal-pharyngo-esophagoscopy and laryngo-tracheo-bronchoscopy) approach to the problem. Using this type of endoscopy, 16 multiple carcinomas have so far been simultaneously discovered among 200 pan-endoscopies."}
{"id": "PMID:462156", "title": "[Experiences with VP-16 in combination with cyclophosphamide or adriamycin in the anaplastic, predominantly small cell bronchogenic carcinoma].", "content": "VP 16-213, the new semisynthetic epipodophyllotoxin, was tested for activity and toxicity in two small pilot series of 24 mainly untreated patients with anaplastic, predominantly small-cell lung cancer. It was given in combination with cyclophosphamide or adriamycin. Among 10 evaluable patients receiving VP-16 + cyclophosphamide (5 orally, 5 intravenously) only 1 good partial remission and 4 partial remissions (less than 50%) were achieved. Toxicity was minimal. In 14 patients on VP-16 and adriamycin, remission was complete in 2, good partial in 5 and lesser partial in 2. The hematological toxicity of the second combination was marked. Severe granulocytopenia required reverse isolation of most patients for periods of up to 2 weeks. The response rate for VP-16 + adriamycin was better than for VP-16 + cyclophosphamide, but lower than expected. Possible explanations are given.", "contents": "[Experiences with VP-16 in combination with cyclophosphamide or adriamycin in the anaplastic, predominantly small cell bronchogenic carcinoma]. VP 16-213, the new semisynthetic epipodophyllotoxin, was tested for activity and toxicity in two small pilot series of 24 mainly untreated patients with anaplastic, predominantly small-cell lung cancer. It was given in combination with cyclophosphamide or adriamycin. Among 10 evaluable patients receiving VP-16 + cyclophosphamide (5 orally, 5 intravenously) only 1 good partial remission and 4 partial remissions (less than 50%) were achieved. Toxicity was minimal. In 14 patients on VP-16 and adriamycin, remission was complete in 2, good partial in 5 and lesser partial in 2. The hematological toxicity of the second combination was marked. Severe granulocytopenia required reverse isolation of most patients for periods of up to 2 weeks. The response rate for VP-16 + adriamycin was better than for VP-16 + cyclophosphamide, but lower than expected. Possible explanations are given."}
{"id": "PMID:462157", "title": "[Effect of 2 retinoids (vitamin A analogs) on human bronchogenic carcinoma in vivo (nu/nu mouse) and in vitro].", "content": "The antitumor effects of 2 aromatic retinoids (analogs of vitamin A) were investigated in human bronchogenic carcinomas grown in vitro and in vivo (serially transplanted in \"nude\" mice). In vitro, bronchogenic carcinoma cells treated with Ro 10-1670 (the free acid of an aromatic retinoid) displayed activation of the lysosomal system and increased cellular breakdown. In vivo, nu/nu-mice treated with Ro 10-9359 (the ethyl-ester of Ro 10-1670 showed inhibition of tumor growth. In the tumors, necrotic areas were intermingled with regions displaying a higher degree of keratinization as compared to those of the controls. The extracellular matrix surrounding the tumor cells of the retinoid-treated animals was increased in volume and electron density.", "contents": "[Effect of 2 retinoids (vitamin A analogs) on human bronchogenic carcinoma in vivo (nu/nu mouse) and in vitro]. The antitumor effects of 2 aromatic retinoids (analogs of vitamin A) were investigated in human bronchogenic carcinomas grown in vitro and in vivo (serially transplanted in \"nude\" mice). In vitro, bronchogenic carcinoma cells treated with Ro 10-1670 (the free acid of an aromatic retinoid) displayed activation of the lysosomal system and increased cellular breakdown. In vivo, nu/nu-mice treated with Ro 10-9359 (the ethyl-ester of Ro 10-1670 showed inhibition of tumor growth. In the tumors, necrotic areas were intermingled with regions displaying a higher degree of keratinization as compared to those of the controls. The extracellular matrix surrounding the tumor cells of the retinoid-treated animals was increased in volume and electron density."}
{"id": "PMID:462158", "title": "[Survival of operated bronchopulmonary neoplasms. Actuarial results after 5 years].", "content": "The actuarial survival of 253 primary bronchopulmonary cancer patients undergoing surgery in Geneva between 1967 and 1976 is studied. The overall post-surgical mortality was 9.88%. The overall survival after exclusion of patients without radical tumor excision was 26.6% at 5 years. The small size of the tumor, the absence of mediastinal extension and an epidermoid histology are the best survival rate factors: 43.2% of stage I patients survive at 5 years against 4.86% of stage III patients. The survival for asymptomatic tumors is 43.9% at 5 years. This suggests the importance of mass screening by radiophotography. Thus, surgery retains its central role in the treatment of this disease, whilst chemo-, radio- and immunotherapy have not yet realized their full potential.", "contents": "[Survival of operated bronchopulmonary neoplasms. Actuarial results after 5 years]. The actuarial survival of 253 primary bronchopulmonary cancer patients undergoing surgery in Geneva between 1967 and 1976 is studied. The overall post-surgical mortality was 9.88%. The overall survival after exclusion of patients without radical tumor excision was 26.6% at 5 years. The small size of the tumor, the absence of mediastinal extension and an epidermoid histology are the best survival rate factors: 43.2% of stage I patients survive at 5 years against 4.86% of stage III patients. The survival for asymptomatic tumors is 43.9% at 5 years. This suggests the importance of mass screening by radiophotography. Thus, surgery retains its central role in the treatment of this disease, whilst chemo-, radio- and immunotherapy have not yet realized their full potential."}
{"id": "PMID:462159", "title": "[Pneumonitis and fibrosis after primary radiotherapy and after combined treatment with chemotherapy in bronchogenic carcinomas].", "content": "36 patients with bronchial carcinoma treated in a randomized study were investigated for late reactions of lung tissue after radiotherapy combined with chemotherapy. The onset and extent of the lesions depended on the primary therapy. In the group of patients who received primary chemotherapy the first signs of radiation pneumonitis and fibrosis were seen earlier than in the group who underwent primary radiotherapy (70 days and 120 days respectively, and 170 days and 237 days respectively). The shrinkage of the lung tissue was greater after primary chemotherapy. However, secondary pneumonia in the irradiated lung volume occurred less frequently in the group who underwent chemotherapy first.", "contents": "[Pneumonitis and fibrosis after primary radiotherapy and after combined treatment with chemotherapy in bronchogenic carcinomas]. 36 patients with bronchial carcinoma treated in a randomized study were investigated for late reactions of lung tissue after radiotherapy combined with chemotherapy. The onset and extent of the lesions depended on the primary therapy. In the group of patients who received primary chemotherapy the first signs of radiation pneumonitis and fibrosis were seen earlier than in the group who underwent primary radiotherapy (70 days and 120 days respectively, and 170 days and 237 days respectively). The shrinkage of the lung tissue was greater after primary chemotherapy. However, secondary pneumonia in the irradiated lung volume occurred less frequently in the group who underwent chemotherapy first."}
{"id": "PMID:462160", "title": "[Postoperative prevention of recurrence with intrapleural Corynebacterium parvum in patients with stage I and II bronchial carcinomas. Ludwig lung cancer study I].", "content": "The Ludwig Lung Cancer Group was created in 1977. Participants are from Austria, Denmark, Germany, Norway, Sweden, Switzerland, and Yugoslavia. 400 patients are randomized yearly. The clinical trial I investigating the role of C. parvum intrapleurally as adjuvant therapy in operable non-small cell lung cancer patients has been closed in February 1979 with a total accrual of 475 patients. The average follow-up time for these cases is approximately 8 months. It is early to make any definitive comparisons of the treatment groups (C. parvum versus placebo). However, it is possible to identify a few high-risk patient groups. Preliminary indications are that surgical T- and N-stage, type of resection, histological type of tumor as well as degree of tumor dedifferentiation (central histology review) are prognostic factors with regard to disease-free interval and survival. In the same, the disease-free interval appears to become shorter for patients experiencing high fever after C. parvum administration. The Ludwig Lung Cancer Group offers a sharp tool for investigating the possible role of adjuvant therapies in non-small cell lung cancer and for gathering new information on the biology of the disease.", "contents": "[Postoperative prevention of recurrence with intrapleural Corynebacterium parvum in patients with stage I and II bronchial carcinomas. Ludwig lung cancer study I]. The Ludwig Lung Cancer Group was created in 1977. Participants are from Austria, Denmark, Germany, Norway, Sweden, Switzerland, and Yugoslavia. 400 patients are randomized yearly. The clinical trial I investigating the role of C. parvum intrapleurally as adjuvant therapy in operable non-small cell lung cancer patients has been closed in February 1979 with a total accrual of 475 patients. The average follow-up time for these cases is approximately 8 months. It is early to make any definitive comparisons of the treatment groups (C. parvum versus placebo). However, it is possible to identify a few high-risk patient groups. Preliminary indications are that surgical T- and N-stage, type of resection, histological type of tumor as well as degree of tumor dedifferentiation (central histology review) are prognostic factors with regard to disease-free interval and survival. In the same, the disease-free interval appears to become shorter for patients experiencing high fever after C. parvum administration. The Ludwig Lung Cancer Group offers a sharp tool for investigating the possible role of adjuvant therapies in non-small cell lung cancer and for gathering new information on the biology of the disease."}
{"id": "PMID:462161", "title": "[Treatment of cirrhotic ascites].", "content": "The treatment of cirrhotic ascites is based on restriction of dietary sodium and fluid intake coupled with diuretics. Ascitic fluid must always be sampled and infection ruled out. Large abdominal paracenteses are dangerous. Most patients with ascites respond to the dietary-diuretic regimen. The clinician must be watchful for overdiuresis. Some patients need diuretics in ever-increasing doses in an attempt to increase urine flow and are at particular risk of functional renal failure. In these diuretic-resistant cases the ascitic fluid can be withdrawn, ultra-filtered and reinfused by means of suitable apparatus. In 1974, LeVeen and co-workers described a peritoneo-venous shunt system which provides more continuous treatment over many months. The morbidity and mortality of this shunt are difficult to determine from published reports. Long-term controlled studies are needed. The beneficial effect on survival of treatment of cirrhotic ascites is still questioned.", "contents": "[Treatment of cirrhotic ascites]. The treatment of cirrhotic ascites is based on restriction of dietary sodium and fluid intake coupled with diuretics. Ascitic fluid must always be sampled and infection ruled out. Large abdominal paracenteses are dangerous. Most patients with ascites respond to the dietary-diuretic regimen. The clinician must be watchful for overdiuresis. Some patients need diuretics in ever-increasing doses in an attempt to increase urine flow and are at particular risk of functional renal failure. In these diuretic-resistant cases the ascitic fluid can be withdrawn, ultra-filtered and reinfused by means of suitable apparatus. In 1974, LeVeen and co-workers described a peritoneo-venous shunt system which provides more continuous treatment over many months. The morbidity and mortality of this shunt are difficult to determine from published reports. Long-term controlled studies are needed. The beneficial effect on survival of treatment of cirrhotic ascites is still questioned."}
{"id": "PMID:462162", "title": "[The physiopathology of ascites].", "content": "Sodium and water retention is constant in decompensated cirrhosis with ascites and edema. Sodium retention is due to several factors. Renal hemodynamic disturbances appear first: decrease in glomerular filtration and renal plasmatic perfusion, redistribution of renal perfusion to the juxtamedullar area where the longer nephrons reabsorb more sodium. Metabolic disorders of estrogens, natriuretic hormonal factor, prostaglandins and the kallikrein-kinin system contribute to greater sodium retention. Water retention is secondary to greater sodium reabsorption and to hyperactivity of the antidiuretic hormone. Sodium and water retention, associated with portal hypertension, with reduced oncotic pressure and with dynamic lymphatic insufficiency, is responsible for the production of ascites. The latter results in a decrease in the effective plasmatic volume, with non-suppression of the renin-angiotensin system, increased aldosterone production and additional sodium retention.", "contents": "[The physiopathology of ascites]. Sodium and water retention is constant in decompensated cirrhosis with ascites and edema. Sodium retention is due to several factors. Renal hemodynamic disturbances appear first: decrease in glomerular filtration and renal plasmatic perfusion, redistribution of renal perfusion to the juxtamedullar area where the longer nephrons reabsorb more sodium. Metabolic disorders of estrogens, natriuretic hormonal factor, prostaglandins and the kallikrein-kinin system contribute to greater sodium retention. Water retention is secondary to greater sodium reabsorption and to hyperactivity of the antidiuretic hormone. Sodium and water retention, associated with portal hypertension, with reduced oncotic pressure and with dynamic lymphatic insufficiency, is responsible for the production of ascites. The latter results in a decrease in the effective plasmatic volume, with non-suppression of the renin-angiotensin system, increased aldosterone production and additional sodium retention."}
{"id": "PMID:462164", "title": "[Mature benign teratomas with malignant tumors and malignant monodermal ovarian teratomas. Anatomo-clinical presentation of 10 cases].", "content": "The clinicopathologic features are presented of 8 malignant neoplasms occurring in adult teratomas, and of 2 malignant monodermal teratomas found in a continuous series of 1029 cases of ovarian adult teratomas. The age of the patients was 44--76 years. The first 8 observations included 6 epidermoid carcinomas, 1 undifferentiated carcinoma and 1 strumal carcinoid. In the epidermoid carcinomas malignancy was recognized peroperatively only in three instances. One tumor had infiltrated the broad ligament on one side and another the urinary bladder. The neoplasm appeared as a mass which thickened the wall of a typical dermoid cyst. Four patients died of the tumor from 3 months to 1 year after operation; one is alive more than 19 years later and one is lost to follow-up. The crucial factor in prognosis is the integrity of the ovarian capsule. The undifferentiated carcinoma, which had infiltrated the pelvic walls, could only be incompletely resected and caused death within 3 1/2 months. The strumal carcinoid was discovered by chance during laparotomy for a far-advanced adenocarcinoma of the pancreas which led to death within 2 months. The two cases of malignant monodermal teratoma comprised 1 thyroid papillary carcinoma and 1 carcinoid. Both patients are alive more than 8 and 5 years after operation. The carcinoid manifested itself by persistant diarrhea which ceased soon after operation and did not recur subsequently.", "contents": "[Mature benign teratomas with malignant tumors and malignant monodermal ovarian teratomas. Anatomo-clinical presentation of 10 cases]. The clinicopathologic features are presented of 8 malignant neoplasms occurring in adult teratomas, and of 2 malignant monodermal teratomas found in a continuous series of 1029 cases of ovarian adult teratomas. The age of the patients was 44--76 years. The first 8 observations included 6 epidermoid carcinomas, 1 undifferentiated carcinoma and 1 strumal carcinoid. In the epidermoid carcinomas malignancy was recognized peroperatively only in three instances. One tumor had infiltrated the broad ligament on one side and another the urinary bladder. The neoplasm appeared as a mass which thickened the wall of a typical dermoid cyst. Four patients died of the tumor from 3 months to 1 year after operation; one is alive more than 19 years later and one is lost to follow-up. The crucial factor in prognosis is the integrity of the ovarian capsule. The undifferentiated carcinoma, which had infiltrated the pelvic walls, could only be incompletely resected and caused death within 3 1/2 months. The strumal carcinoid was discovered by chance during laparotomy for a far-advanced adenocarcinoma of the pancreas which led to death within 2 months. The two cases of malignant monodermal teratoma comprised 1 thyroid papillary carcinoma and 1 carcinoid. Both patients are alive more than 8 and 5 years after operation. The carcinoid manifested itself by persistant diarrhea which ceased soon after operation and did not recur subsequently."}
{"id": "PMID:462165", "title": "[Severe hemolytic anemia after mitral valvuloplasty].", "content": "A patient with mitral insufficiency is described who developed severe hemolytic anemia six months after mitral valvuloplasty. Various laboratory tests suggested that the cause of the hemolysis was mechanical. A second heart operation was therefore performed which revealed intact valve function. In view of the mechanical cause of hemolysis a bioprosthesis was implanted but the patient died shortly after this operation. Since similar cases are not found in the literature the authors invite discussion of the course in this case.", "contents": "[Severe hemolytic anemia after mitral valvuloplasty]. A patient with mitral insufficiency is described who developed severe hemolytic anemia six months after mitral valvuloplasty. Various laboratory tests suggested that the cause of the hemolysis was mechanical. A second heart operation was therefore performed which revealed intact valve function. In view of the mechanical cause of hemolysis a bioprosthesis was implanted but the patient died shortly after this operation. Since similar cases are not found in the literature the authors invite discussion of the course in this case."}
{"id": "PMID:462167", "title": "[Near-drowning in children].", "content": "From 1971 to 1977 a total of 32 nearly-drowned children were admitted to the University Pediatric Hospital, Berne, and the Children's Hospital, Lucerne. 5 died within 15 h to 10 days. Neurological damage was seen in 5 cases. 20 children were under 5 years of age and only 8 over 7 years. All cases with fatal consequences or severe cerebral sequel had cardiac arrest on admission. A maximal possible submersion time for survival without consequences has not been found. The pathophysiology of fresh water drowning and current treatment are summarized. Situations leading to near drowning are specified and some aspects of prognosis discussed.", "contents": "[Near-drowning in children]. From 1971 to 1977 a total of 32 nearly-drowned children were admitted to the University Pediatric Hospital, Berne, and the Children's Hospital, Lucerne. 5 died within 15 h to 10 days. Neurological damage was seen in 5 cases. 20 children were under 5 years of age and only 8 over 7 years. All cases with fatal consequences or severe cerebral sequel had cardiac arrest on admission. A maximal possible submersion time for survival without consequences has not been found. The pathophysiology of fresh water drowning and current treatment are summarized. Situations leading to near drowning are specified and some aspects of prognosis discussed."}
{"id": "PMID:462168", "title": "[The echocardiogram in mitral anulus calcification].", "content": "In agreement with the known high incidence of mitral anulus calcification in elderly patients, the authors have found corresponding calcifications in echocardiographic examinations in 61 patients as the single or additional cause of an abnormal systolic or diastolic murmur. The intracardiac calcifications were known of from the chest X-ray only in 6 patients. The echocardiograms served to locate and estimate the amount of the calcification. Calcifications of the aortic cusps, found in 75% of patients, may be of degenerative origin. The echocardiographic presentation of the degenerative calcifications can be misinterpreted either as postrheumatic valvular disease, posterior pericardial effusion or hypertrophic cardiomyopathy. Registration by continuous sector scan was necessary to differentiate these entities.", "contents": "[The echocardiogram in mitral anulus calcification]. In agreement with the known high incidence of mitral anulus calcification in elderly patients, the authors have found corresponding calcifications in echocardiographic examinations in 61 patients as the single or additional cause of an abnormal systolic or diastolic murmur. The intracardiac calcifications were known of from the chest X-ray only in 6 patients. The echocardiograms served to locate and estimate the amount of the calcification. Calcifications of the aortic cusps, found in 75% of patients, may be of degenerative origin. The echocardiographic presentation of the degenerative calcifications can be misinterpreted either as postrheumatic valvular disease, posterior pericardial effusion or hypertrophic cardiomyopathy. Registration by continuous sector scan was necessary to differentiate these entities."}
{"id": "PMID:462169", "title": "[Kirner's deformity: an isolated malformation of the third phalanx of the little finger].", "content": "A typical growth deformity of the third phalanx of the 5th finger known in the literature as \"Kirner's deformity\" has been observed over 5 years in 4 patients (there are now 5 patients) at the Clinic for Hand Surgery in St. Gall. Almost all reports (39 cases) are in the English-language literature. It is felt that apart from esthetic problems functional deficiency is of considerable relevance, and the only existing therapy mentioned in the literature (osteotomy) has been supplemented by localized partial epiphysiodesis by microsurgical operative technique.", "contents": "[Kirner's deformity: an isolated malformation of the third phalanx of the little finger]. A typical growth deformity of the third phalanx of the 5th finger known in the literature as \"Kirner's deformity\" has been observed over 5 years in 4 patients (there are now 5 patients) at the Clinic for Hand Surgery in St. Gall. Almost all reports (39 cases) are in the English-language literature. It is felt that apart from esthetic problems functional deficiency is of considerable relevance, and the only existing therapy mentioned in the literature (osteotomy) has been supplemented by localized partial epiphysiodesis by microsurgical operative technique."}
{"id": "PMID:462170", "title": "[Dissociation of human factor VIII and recombination of the fragments VIII-C and VIII-AG].", "content": "Human factor VIII has been dissociated into fragment VIII-C and fragment VIII-AG in the presence of calcium chloride (0.25 M). After removing the calcium, recombination of the molecule is obtained. Fragment VIII-C can be activated by thrombin in a similar manner to the coagulant activity of whole factor VIII. This activation is calcium-dependent. In rabbits immunized against purified F VIII, VIII-C and VIII-AG specific antisera against these three entities were obtained.", "contents": "[Dissociation of human factor VIII and recombination of the fragments VIII-C and VIII-AG]. Human factor VIII has been dissociated into fragment VIII-C and fragment VIII-AG in the presence of calcium chloride (0.25 M). After removing the calcium, recombination of the molecule is obtained. Fragment VIII-C can be activated by thrombin in a similar manner to the coagulant activity of whole factor VIII. This activation is calcium-dependent. In rabbits immunized against purified F VIII, VIII-C and VIII-AG specific antisera against these three entities were obtained."}
{"id": "PMID:462171", "title": "[Sacroiliac changes, HLA-B27 negative, in primary hyperparathyroidism and osteomalacia].", "content": "Radiological sacroiliac (SI) changes were found in 3 patients, 2 with primary hyperparathyroidism (1 also with associated chondrocalcinosis) and 1 with osteomalacia. Osteomalacia was due to celiac disease. None of the 3 patients, all females, had a history of psoriasis, urethritis, iritis or chronic colitis. There was no renal function impairment. Peripheral joints were affected in the patient with associated condrocalcinosis. HLA B 27 was negative in all cases. Low back pain and vertebral stiffness were present in the patient with osteomalacia. A dramatic improvement in pain and stiffness ensued after vitamin D injections. These SI lesions, which may simulate ankylosing spondylitis, were attributable to subchondral bone changes related to the metabolic bone diseases. In the case of osteomalacia the SI lesions were predominantly on the right side, where there was a Looser's zone on the ischial ramus suggesting that pseudofractures could be a cause of SI changes. Metabolic osseous diseases such as osteomalacia or primary hyperparathyroidism should be investigated in cases of HLA B 27 negative radiological \"sacroiliitis\".", "contents": "[Sacroiliac changes, HLA-B27 negative, in primary hyperparathyroidism and osteomalacia]. Radiological sacroiliac (SI) changes were found in 3 patients, 2 with primary hyperparathyroidism (1 also with associated chondrocalcinosis) and 1 with osteomalacia. Osteomalacia was due to celiac disease. None of the 3 patients, all females, had a history of psoriasis, urethritis, iritis or chronic colitis. There was no renal function impairment. Peripheral joints were affected in the patient with associated condrocalcinosis. HLA B 27 was negative in all cases. Low back pain and vertebral stiffness were present in the patient with osteomalacia. A dramatic improvement in pain and stiffness ensued after vitamin D injections. These SI lesions, which may simulate ankylosing spondylitis, were attributable to subchondral bone changes related to the metabolic bone diseases. In the case of osteomalacia the SI lesions were predominantly on the right side, where there was a Looser's zone on the ischial ramus suggesting that pseudofractures could be a cause of SI changes. Metabolic osseous diseases such as osteomalacia or primary hyperparathyroidism should be investigated in cases of HLA B 27 negative radiological \"sacroiliitis\"."}
{"id": "PMID:462173", "title": "[Clinical pathology of colonic mucosal polyps].", "content": "Of 636 polyps removed during endoscopy between 1973 and 1975 at the University Hospital, Zurich, 36 (5.5%) were of the hyperplastic and 18 (2.8%) of the juvenile type. One polyp was seen in a female patient with Peutz-Jeghers syndrome. The vast majority of the polyps (581, 91.5%) were neoplastic in origin; 70% were tubular adenomas, 16% villous adenomas, and 14% intermediate forms or tubulo-villous adenomas. On the basis of a continuous spectrum in histologic structure and similar cellular dedifferentiation, these three types of adenoma may be viewed as different forms in the development of the same neoplastic process. Hyperplastic and hamartomatous polyps are innocuous, whereas the neoplastic forms may well turn aggressive. Malignant change was observed in 5.1% of our material, particularly in villous adenomas and in polyps exceeding 1 cm in diameter. The presence or absence of invasive growth through the muscularis mucosae is of prime importance for therapy. In accordance with WHO nomenclature, the term carcinoma is used only in the presence of such infiltration. If, in addition, the tumor tissue is not well differentiated, additional segmental resection may be required. The term \"focal carcinoma\" is no longer in use and has been replaced by \"severe focal atypia\". In these cases, primary polypectomy for diagnostic purposes is also the optimal therapy, and is as effective here as in cases of benign adenoma.", "contents": "[Clinical pathology of colonic mucosal polyps]. Of 636 polyps removed during endoscopy between 1973 and 1975 at the University Hospital, Zurich, 36 (5.5%) were of the hyperplastic and 18 (2.8%) of the juvenile type. One polyp was seen in a female patient with Peutz-Jeghers syndrome. The vast majority of the polyps (581, 91.5%) were neoplastic in origin; 70% were tubular adenomas, 16% villous adenomas, and 14% intermediate forms or tubulo-villous adenomas. On the basis of a continuous spectrum in histologic structure and similar cellular dedifferentiation, these three types of adenoma may be viewed as different forms in the development of the same neoplastic process. Hyperplastic and hamartomatous polyps are innocuous, whereas the neoplastic forms may well turn aggressive. Malignant change was observed in 5.1% of our material, particularly in villous adenomas and in polyps exceeding 1 cm in diameter. The presence or absence of invasive growth through the muscularis mucosae is of prime importance for therapy. In accordance with WHO nomenclature, the term carcinoma is used only in the presence of such infiltration. If, in addition, the tumor tissue is not well differentiated, additional segmental resection may be required. The term \"focal carcinoma\" is no longer in use and has been replaced by \"severe focal atypia\". In these cases, primary polypectomy for diagnostic purposes is also the optimal therapy, and is as effective here as in cases of benign adenoma."}
{"id": "PMID:462174", "title": "Dynamics of skeletal pattern formation in developing chick limb.", "content": "During development of the embryonic chick limb the skeletal pattern is laid out as cartilaginous primordia, which emerge in a proximodistal sequence over a period of 4 days. The differentiation of cartilage is preceded by changes in cellular contacts at specific locations in the precartilage mesenchyme. Under realistic assumptions, the biosynthesis and diffusion through the extracellular matrix of a cell surface protein, such as fibronectin, will lead to spatial patterns of this molecule that could be the basis of the emergent primordia. As cellular differentiation proceeds, the size of the mesenchymal diffusion chamber is reduced in descrete steps, leading to sequential reorganizations of the morphogen pattern. The successive patterns correspond to observed rows of skeletal elements, whose emergence, in theory and in practice, depends on the maintenance of a unique boundary condition at the limb bud apex.", "contents": "Dynamics of skeletal pattern formation in developing chick limb. During development of the embryonic chick limb the skeletal pattern is laid out as cartilaginous primordia, which emerge in a proximodistal sequence over a period of 4 days. The differentiation of cartilage is preceded by changes in cellular contacts at specific locations in the precartilage mesenchyme. Under realistic assumptions, the biosynthesis and diffusion through the extracellular matrix of a cell surface protein, such as fibronectin, will lead to spatial patterns of this molecule that could be the basis of the emergent primordia. As cellular differentiation proceeds, the size of the mesenchymal diffusion chamber is reduced in descrete steps, leading to sequential reorganizations of the morphogen pattern. The successive patterns correspond to observed rows of skeletal elements, whose emergence, in theory and in practice, depends on the maintenance of a unique boundary condition at the limb bud apex."}
{"id": "PMID:462175", "title": "Nonpneumonic, short-incubation-period Legionellosis (Pontiac fever) in men who cleaned a steam turbine condenser.", "content": "Pontiac fever affected ten men who had cleaned a steam turbine condenser with compressed air. Previous epidemics of Pontiac fever and Legionnaires' disease--both caused by Legionella Pneumophila (proposed sp. nov.)--involved \"airborne spread\" from air-conditioning cooling towers or evaporative condensers. Aerosols of contaminated water in heat-rejection systems appear to be important sources of epidemic legionellosis.", "contents": "Nonpneumonic, short-incubation-period Legionellosis (Pontiac fever) in men who cleaned a steam turbine condenser. Pontiac fever affected ten men who had cleaned a steam turbine condenser with compressed air. Previous epidemics of Pontiac fever and Legionnaires' disease--both caused by Legionella Pneumophila (proposed sp. nov.)--involved \"airborne spread\" from air-conditioning cooling towers or evaporative condensers. Aerosols of contaminated water in heat-rejection systems appear to be important sources of epidemic legionellosis."}
{"id": "PMID:462176", "title": "4-aminobutyrate:2-oxoglutarate aminotransferase in blood platelets.", "content": "Platelet lysates obtained from blood of humans, dogs, and rats catalyzed the transamination of 4-aminobutyrate with 2-oxoglutarate as cosubstrate. Human platelet 4-aminobutyrate:2-oxoglutarate aminotransferase (36.5 +/- 3.2 picomoles per minute per milligram of platelet protein) resembled the brain enzyme in kinetic properties and in response to cofactors and inhibitors.", "contents": "4-aminobutyrate:2-oxoglutarate aminotransferase in blood platelets. Platelet lysates obtained from blood of humans, dogs, and rats catalyzed the transamination of 4-aminobutyrate with 2-oxoglutarate as cosubstrate. Human platelet 4-aminobutyrate:2-oxoglutarate aminotransferase (36.5 +/- 3.2 picomoles per minute per milligram of platelet protein) resembled the brain enzyme in kinetic properties and in response to cofactors and inhibitors."}
{"id": "PMID:462177", "title": "Development of the rat's uncrossed retinotectal pathway and its relation to plasticity studies.", "content": "In the normal newborn rat the retinotectal pathway from each eye distributes across the whole area of both the ipsilateral and contralateral superior colliculus. Most of the ipsilateral projection retracts during the first ten postnatal days to produce the normal adult pattern, but retraction fails to occur if one eye is removed at birth.", "contents": "Development of the rat's uncrossed retinotectal pathway and its relation to plasticity studies. In the normal newborn rat the retinotectal pathway from each eye distributes across the whole area of both the ipsilateral and contralateral superior colliculus. Most of the ipsilateral projection retracts during the first ten postnatal days to produce the normal adult pattern, but retraction fails to occur if one eye is removed at birth."}
{"id": "PMID:462178", "title": "Synaptic proteins after electroconvulsive stimulation.", "content": "Quantitative immunoelectrophoresis of rat brain synaptic proteins following a series of electroconvulsive stimulations demonstrated changes suggestive of an increase in the number of synaptic vesicles, in a preparedness for glycolytic demands, and a delayed development of a certain area in the brain. The increased synaptic remodeling may be important for the action of electroconvulsive therapy.", "contents": "Synaptic proteins after electroconvulsive stimulation. Quantitative immunoelectrophoresis of rat brain synaptic proteins following a series of electroconvulsive stimulations demonstrated changes suggestive of an increase in the number of synaptic vesicles, in a preparedness for glycolytic demands, and a delayed development of a certain area in the brain. The increased synaptic remodeling may be important for the action of electroconvulsive therapy."}
{"id": "PMID:462179", "title": "Differential behavioral and biochemical effects of right and left hemispheric cerebral infarction in the rat.", "content": "Following ligation of the right middle cerebral artery, rats were hyperactive for 2 to 3 weeks whether activity was measured by running wheel revolutions or open field observations. Assays of brain catecholamines revealed 30 percent reductions of norepinephrine in the injured and uninjured cortex and locus coeruleus and a 20 percent reduction of dopamine in the substantia nigra. In contrast, rats with left middle cerebral artery ligations did not become hyperactive and did not show any significant change in catecholamines in any of the brain areas studied. Right and left hemispheric infarctions were comparable in their locations and extent of tissue damage. This lateralization of behavioral and biochemical response to cerebral infarction may be the consequence of anatomical or physiological asymmetries in the brain.", "contents": "Differential behavioral and biochemical effects of right and left hemispheric cerebral infarction in the rat. Following ligation of the right middle cerebral artery, rats were hyperactive for 2 to 3 weeks whether activity was measured by running wheel revolutions or open field observations. Assays of brain catecholamines revealed 30 percent reductions of norepinephrine in the injured and uninjured cortex and locus coeruleus and a 20 percent reduction of dopamine in the substantia nigra. In contrast, rats with left middle cerebral artery ligations did not become hyperactive and did not show any significant change in catecholamines in any of the brain areas studied. Right and left hemispheric infarctions were comparable in their locations and extent of tissue damage. This lateralization of behavioral and biochemical response to cerebral infarction may be the consequence of anatomical or physiological asymmetries in the brain."}
{"id": "PMID:462180", "title": "Differences in neural organization between individuals with inverted and noninverted handwriting postures.", "content": "Levy's hypothesis that movements of the distal musculature are controlled by ipsilateral motor projections in subjects with inverted writing posture was tested in a reaction-time experiment with lateralized auditory, tactual, and visual stimulation. Subjects were required to depress a response key with the left or right index finger when they detected a stimulus in either the left or right sensory field. Writers with noninverted posture responded quickest to stimuli on the same side as the responding hand in all modalities tested, whereas inverted writers showed this pattern only in auditory and tactual modalities. In the visual modality, they responded quickest to stimuli on the side opposite the responding hand. Because Levy's hypothesis predicts the latter effect in all modalities for inverted writers, it is challenged by our results, which suggest that inverted writers may be characterized by anomalous visual or visuomotor organization.", "contents": "Differences in neural organization between individuals with inverted and noninverted handwriting postures. Levy's hypothesis that movements of the distal musculature are controlled by ipsilateral motor projections in subjects with inverted writing posture was tested in a reaction-time experiment with lateralized auditory, tactual, and visual stimulation. Subjects were required to depress a response key with the left or right index finger when they detected a stimulus in either the left or right sensory field. Writers with noninverted posture responded quickest to stimuli on the same side as the responding hand in all modalities tested, whereas inverted writers showed this pattern only in auditory and tactual modalities. In the visual modality, they responded quickest to stimuli on the side opposite the responding hand. Because Levy's hypothesis predicts the latter effect in all modalities for inverted writers, it is challenged by our results, which suggest that inverted writers may be characterized by anomalous visual or visuomotor organization."}
{"id": "PMID:462181", "title": "Delayed neurotoxicity of phenylphosphonothioate esters.", "content": "Administration of a single oral dose of five phenylphosphonothioate esters produced delayed neurotoxicity in hens; their potency was, in descending order, cyanofenphos, EPN, desbromoleptophos, leptophos, and EPBP (Seven). Histological examination showed that in some hens there was marked axonal and myelin degeneration in the spinal cord and peripheral nerves. The results suggest that delayed neurotoxicity may be a general feature of phenylphosphonothioate insecticides.", "contents": "Delayed neurotoxicity of phenylphosphonothioate esters. Administration of a single oral dose of five phenylphosphonothioate esters produced delayed neurotoxicity in hens; their potency was, in descending order, cyanofenphos, EPN, desbromoleptophos, leptophos, and EPBP (Seven). Histological examination showed that in some hens there was marked axonal and myelin degeneration in the spinal cord and peripheral nerves. The results suggest that delayed neurotoxicity may be a general feature of phenylphosphonothioate insecticides."}
{"id": "PMID:462182", "title": "Drug discrimination training with progressively lowered doses.", "content": "Rats were trained to discriminate drug from no-drug conditions in a two-lever operant task. Moderately high dosages were used initially. Whenever the discrimination was learned, training was continued with progressively reduced dosages. Eventually the rats discriminated extremely low doses of phenobarbital, chlordiazepoxide, cyclazocine, and fentanyl.", "contents": "Drug discrimination training with progressively lowered doses. Rats were trained to discriminate drug from no-drug conditions in a two-lever operant task. Moderately high dosages were used initially. Whenever the discrimination was learned, training was continued with progressively reduced dosages. Eventually the rats discriminated extremely low doses of phenobarbital, chlordiazepoxide, cyclazocine, and fentanyl."}
{"id": "PMID:462183", "title": "Superstitious bar pressing in hippocampal and septal rats.", "content": "Unlike normal animals or those with sham lesions, rats with hippocampal and septal lesions behaved in an operant chamber as if a dependency existed between pellet delivery and their behavior, despite the fact that reinforcement was based on time, not behavior, and was therefore free. This superstitious behavior did not result from a general inability to inhibit responding, as responding rapidly ceased when the pellets were discontinued. These findings suggest that the hippocampus integrates information regarding response-reinforcer relations, which in the normal rat permits superfluous operant behavior to be eliminated.", "contents": "Superstitious bar pressing in hippocampal and septal rats. Unlike normal animals or those with sham lesions, rats with hippocampal and septal lesions behaved in an operant chamber as if a dependency existed between pellet delivery and their behavior, despite the fact that reinforcement was based on time, not behavior, and was therefore free. This superstitious behavior did not result from a general inability to inhibit responding, as responding rapidly ceased when the pellets were discontinued. These findings suggest that the hippocampus integrates information regarding response-reinforcer relations, which in the normal rat permits superfluous operant behavior to be eliminated."}
{"id": "PMID:462184", "title": "Metabolic mapping of functional activity in the hypothalamo-neurohypophysial system of the rat.", "content": "Physiological stimulation of the hypothalamo-neurohypophysial system by salt loading of rats resulted in a dramatically increased glucose utilization in the posterior pituitary but not in the paraventricular or supraoptic nuclei. The good correlation between glucose utilization and neural activity in the posterior pituitary (that is, nerve terminals) contrasted with the lack of correlation in the paraventricular and supraoptic nuclei (that is, the sites of the cell bodies of the same neurons). This difference in the metabolic response to functional activity between the two regions of these neurons can be explained by the differences in surface-to-volume ratios of these regions.", "contents": "Metabolic mapping of functional activity in the hypothalamo-neurohypophysial system of the rat. Physiological stimulation of the hypothalamo-neurohypophysial system by salt loading of rats resulted in a dramatically increased glucose utilization in the posterior pituitary but not in the paraventricular or supraoptic nuclei. The good correlation between glucose utilization and neural activity in the posterior pituitary (that is, nerve terminals) contrasted with the lack of correlation in the paraventricular and supraoptic nuclei (that is, the sites of the cell bodies of the same neurons). This difference in the metabolic response to functional activity between the two regions of these neurons can be explained by the differences in surface-to-volume ratios of these regions."}
{"id": "PMID:462188", "title": "Assessment of diagnostic technologies.", "content": "A general protocol for rigorous evaluation of diagnostic systems in medicine was applied successfully in a comparative study of two radiologic techniques. Accuracies of computed tomography and radionuclide scanning in detecting, localizing, and diagnosing brain lesions were assessed with a sample of patients in whom tumor had been suspected. The principal means of analysis was the \"relative operating characteristic,\" which is unique in providing a measure of accuracy that is largely independent of decision biases. Computed tomography was found to be substantially more accurate than radionuclide scanning.", "contents": "Assessment of diagnostic technologies. A general protocol for rigorous evaluation of diagnostic systems in medicine was applied successfully in a comparative study of two radiologic techniques. Accuracies of computed tomography and radionuclide scanning in detecting, localizing, and diagnosing brain lesions were assessed with a sample of patients in whom tumor had been suspected. The principal means of analysis was the \"relative operating characteristic,\" which is unique in providing a measure of accuracy that is largely independent of decision biases. Computed tomography was found to be substantially more accurate than radionuclide scanning."}
{"id": "PMID:462191", "title": "Retinotectal projection in goldfish to an inappropriate region with a reversal in polarity.", "content": "An abnormal, ipsilateral projection was formed by deflecting optic fibers that normally innervate the posterior part of one tectum into the anterior end of the opposite tectum. When anterior recipient tectum was simultaneously denervate, the deflected fibers formed a retinotopic map in this region that was reversed with respect to the anterior-posterior tectal axis.", "contents": "Retinotectal projection in goldfish to an inappropriate region with a reversal in polarity. An abnormal, ipsilateral projection was formed by deflecting optic fibers that normally innervate the posterior part of one tectum into the anterior end of the opposite tectum. When anterior recipient tectum was simultaneously denervate, the deflected fibers formed a retinotopic map in this region that was reversed with respect to the anterior-posterior tectal axis."}
{"id": "PMID:462192", "title": "GABA receptors in clonal cell lines: a model for study of benzodiazepine action at molecular level.", "content": "A \"recptor unit\" for gamma-aminobutyric acid (GABA), which includes brainlike receptor binding sites for tritium-labeled GABA and benzodiazepines (diazepam, clonazepam, and flunitrazepam) and a thermostable endogenous protein (GABA modulin) that inhibits both GABA and benzodiazepine binding, has been demonstrated in membranes prepared from NB2a neuroblastoma and C6 glioma clonal cell lines. In these cells, as in brain, diazepam (1 micromolar) prevents the effect of GABA modulin, and in turn GABA (0.oma and, to a lesser extent, the glioma cells represent a suitable model to study the interactions and the sequence of membrane and intracellular events triggered by the stimulation of benzodiazepine and GABA receptors.", "contents": "GABA receptors in clonal cell lines: a model for study of benzodiazepine action at molecular level. A \"recptor unit\" for gamma-aminobutyric acid (GABA), which includes brainlike receptor binding sites for tritium-labeled GABA and benzodiazepines (diazepam, clonazepam, and flunitrazepam) and a thermostable endogenous protein (GABA modulin) that inhibits both GABA and benzodiazepine binding, has been demonstrated in membranes prepared from NB2a neuroblastoma and C6 glioma clonal cell lines. In these cells, as in brain, diazepam (1 micromolar) prevents the effect of GABA modulin, and in turn GABA (0.oma and, to a lesser extent, the glioma cells represent a suitable model to study the interactions and the sequence of membrane and intracellular events triggered by the stimulation of benzodiazepine and GABA receptors."}
{"id": "PMID:462193", "title": "Menopausal flushes: a neuroendocrine link with pulsatile luteninizing hormone secreation.", "content": "Menopausal flush episodes were found to be invariably associated with the initiation of pulsatile pituitary release of luteinizing hormone. This was not accompanied by a significant change in circulating catecholamine or prolactin concentrations. Since pulsatile luteinizing hormone release results from episodic secretion of luteinizing hormone releasing factor by the hypothalamus, these findings suggest a link between the neuroendocrine mechanisms that initiate such episodic secretion and those responsible for the onset of flush episodes.", "contents": "Menopausal flushes: a neuroendocrine link with pulsatile luteninizing hormone secreation. Menopausal flush episodes were found to be invariably associated with the initiation of pulsatile pituitary release of luteinizing hormone. This was not accompanied by a significant change in circulating catecholamine or prolactin concentrations. Since pulsatile luteinizing hormone release results from episodic secretion of luteinizing hormone releasing factor by the hypothalamus, these findings suggest a link between the neuroendocrine mechanisms that initiate such episodic secretion and those responsible for the onset of flush episodes."}
{"id": "PMID:462194", "title": "alpha-Melanocyte-stimulating hormone: reduction in adult rat brain after monosodium glutamate treatment of neonates.", "content": "Intraperitoneal injection of monosodium glutamate in neonatal rates resulted in a 90 percent loss of alpha-melanocyte-stimulating hormone in hypothalamic and extrahypothalamic areas of the brain, whereas the amount of hormone in the pituitary gland did not change. The dramatic reduction of alpha-melanocyte-stimulating hormone in the brain suggests that is primary source there is the neuronal perikarya of the arcuate nucleus.", "contents": "alpha-Melanocyte-stimulating hormone: reduction in adult rat brain after monosodium glutamate treatment of neonates. Intraperitoneal injection of monosodium glutamate in neonatal rates resulted in a 90 percent loss of alpha-melanocyte-stimulating hormone in hypothalamic and extrahypothalamic areas of the brain, whereas the amount of hormone in the pituitary gland did not change. The dramatic reduction of alpha-melanocyte-stimulating hormone in the brain suggests that is primary source there is the neuronal perikarya of the arcuate nucleus."}
{"id": "PMID:462246", "title": "Preparing the health professional for community health.", "content": "An assessment is made of an initial experience of undergraduate and postgraduate preparation increasingly directed to priority areas in community health. It is measured against set objectives, interrelated factors and a WHO viewpoint of target areas.", "contents": "Preparing the health professional for community health. An assessment is made of an initial experience of undergraduate and postgraduate preparation increasingly directed to priority areas in community health. It is measured against set objectives, interrelated factors and a WHO viewpoint of target areas."}
{"id": "PMID:462248", "title": "[The contributions of homeland health services to plural development].", "content": "A brief outline is given of the impact of health services on plural development in the self-governing Black states of South Africa. The importance of community involvement and primary health care in the development process is emphasized. The concept of sustained interterritorial co-operation in health matters in Southern Africa provides the only effective means of dealing with problems which ignore political boundaries.", "contents": "[The contributions of homeland health services to plural development]. A brief outline is given of the impact of health services on plural development in the self-governing Black states of South Africa. The importance of community involvement and primary health care in the development process is emphasized. The concept of sustained interterritorial co-operation in health matters in Southern Africa provides the only effective means of dealing with problems which ignore political boundaries."}
{"id": "PMID:462249", "title": "Trends in four major communicable diseases.", "content": "The official notifications of the Department of Health pertaining to typhoid fever, tuberculosis, poliomyelitis and malaria are reviewed. Major findings of the epidemiological analysis of the data include the need for establishing the exact mode of transmission of typhoid, the need for assessing upper and lower limits of the expected case load in respect of tuberculosis over the next 5--10 years, the demonstration of the dramatic decline in the number of cases of poliomyelitis and the equally dramatic upsurge of malaria in 1977 and 1978. The need for an integrated, responsive and fully co-ordinated epidemiological service for the Republic of South Africa is emphasized.", "contents": "Trends in four major communicable diseases. The official notifications of the Department of Health pertaining to typhoid fever, tuberculosis, poliomyelitis and malaria are reviewed. Major findings of the epidemiological analysis of the data include the need for establishing the exact mode of transmission of typhoid, the need for assessing upper and lower limits of the expected case load in respect of tuberculosis over the next 5--10 years, the demonstration of the dramatic decline in the number of cases of poliomyelitis and the equally dramatic upsurge of malaria in 1977 and 1978. The need for an integrated, responsive and fully co-ordinated epidemiological service for the Republic of South Africa is emphasized."}
{"id": "PMID:462250", "title": "[The protection of health in a changing environment].", "content": "One of the main functions of the Department of Health is to 'take steps for the promotion of a safe and healthy environment'. Air and water pollution, unhygienic conditions, incorrect use of radiation equipment, detrimental working conditions together with injudicious use of consumer products are but a few facets which have to be attended to in this all-embracing brief of the Health Act No. 63 of 1977. This is achieved by giving attention to scientific principles and effective co-operation between central and local authorities as well as the private sector.", "contents": "[The protection of health in a changing environment]. One of the main functions of the Department of Health is to 'take steps for the promotion of a safe and healthy environment'. Air and water pollution, unhygienic conditions, incorrect use of radiation equipment, detrimental working conditions together with injudicious use of consumer products are but a few facets which have to be attended to in this all-embracing brief of the Health Act No. 63 of 1977. This is achieved by giving attention to scientific principles and effective co-operation between central and local authorities as well as the private sector."}
{"id": "PMID:462252", "title": "Health education--quo vadis?", "content": "Various implications for health education in relation to current approaches to community medicine are reviewed. Health education activities and needs in the Republic of South Africa are surveyed. Suggested solutions to existing problems include the development of more co-ordinated planning and implementation and evaluation of health education programmes. The urgent need for both undergraduate and postgraduate teaching facilities is stressed. It is suggested that the role and services of the South African National Council for Health Education should be reviewed, so that the Council's resources can in future be better utilized.", "contents": "Health education--quo vadis? Various implications for health education in relation to current approaches to community medicine are reviewed. Health education activities and needs in the Republic of South Africa are surveyed. Suggested solutions to existing problems include the development of more co-ordinated planning and implementation and evaluation of health education programmes. The urgent need for both undergraduate and postgraduate teaching facilities is stressed. It is suggested that the role and services of the South African National Council for Health Education should be reviewed, so that the Council's resources can in future be better utilized."}
{"id": "PMID:462263", "title": "Effects of metronidazole on certain functions of human blood neutrophils and lymphocytes.", "content": "The effects of metronidazole at varying concentrations (10(-7)M - 10(-2)M) on certain cellular immune functions were assessed in vitro. Neutrophil chemotaxis to endotoxin-activated autologous serum, random motility and postphagocytic metabolic activity (hexose monophosphate shunt activity, myeloperoxidase-mediated protein iodination and glycolysis) were unaffected. Likewise, metronidazole had no detectable effects on lymphocyte-active E-rosette formation, mitogen-induced DNA synthesis and mitogen-induced protein synthesis.", "contents": "Effects of metronidazole on certain functions of human blood neutrophils and lymphocytes. The effects of metronidazole at varying concentrations (10(-7)M - 10(-2)M) on certain cellular immune functions were assessed in vitro. Neutrophil chemotaxis to endotoxin-activated autologous serum, random motility and postphagocytic metabolic activity (hexose monophosphate shunt activity, myeloperoxidase-mediated protein iodination and glycolysis) were unaffected. Likewise, metronidazole had no detectable effects on lymphocyte-active E-rosette formation, mitogen-induced DNA synthesis and mitogen-induced protein synthesis."}
{"id": "PMID:462264", "title": "Rare and unusual manifestations of Crohn's disease with pyoderma gangrenosum and sclerosing cholangitis.", "content": "Crohn's ileocolitis manifested in 2 Black patients with the exceptionally rare complications of pyoderma gangrenosum and sclerosing cholangitis. It is emphasized that Crohn's disease is very rare in the Black population of sub-Saharan Africa, and, as far as we are aware, no patient with large-bowel involvement has hitherto been reported.", "contents": "Rare and unusual manifestations of Crohn's disease with pyoderma gangrenosum and sclerosing cholangitis. Crohn's ileocolitis manifested in 2 Black patients with the exceptionally rare complications of pyoderma gangrenosum and sclerosing cholangitis. It is emphasized that Crohn's disease is very rare in the Black population of sub-Saharan Africa, and, as far as we are aware, no patient with large-bowel involvement has hitherto been reported."}
{"id": "PMID:462265", "title": "Benign duodenocolic fistula. A report of 3 cases.", "content": "Duodenocolic fistulae, although often malignant in aetiology, may result from a wide range of relatively benign disease processes. A report is given of 3 patients with benign duodenocolic fistulae, in 2 on the basis of tuberculosis and in 1 after a duodenal ulcer. The subject is reviewed and the ease of management and good prognosis are stressed.", "contents": "Benign duodenocolic fistula. A report of 3 cases. Duodenocolic fistulae, although often malignant in aetiology, may result from a wide range of relatively benign disease processes. A report is given of 3 patients with benign duodenocolic fistulae, in 2 on the basis of tuberculosis and in 1 after a duodenal ulcer. The subject is reviewed and the ease of management and good prognosis are stressed."}
{"id": "PMID:462273", "title": "Diagnosis of upper gastro-intestinal lesions by endoscopy, cytology and biopsy.", "content": "A prospective consecutive series of 210 upper gastrointestinal endoscopic procedures, at which material for both cytological and histopathological examination was obtained, was conducted at Baragwanath Hospital. The purpose of the study was to determine the respective and combined diagnostic value of endoscopic visualization, cytological brushings and histological sections in benign and malignant lesions of the oesophagus and stomach; and in particular to assess the contribution, if any, of exfoliative cytology to the diagnostic regimen. Endoscopic visualization alone was inconclusive or incorrect in 13% of procedures, cytology alone in 9%, and biopsy alone in 12% (the latter 2 figures corrected for adequacy of material submitted). When assessed together, the diagnostic accuracy achieved by all 3 techniques was 99%. The major contribution of cytology was the correct positive diagnosis in 20% of procedures, performed on patients with malignant lesions, whose initial biopsy specimen was inadequate or normal. It is concluded that for maximal diagnostic accuracy of upper gastro-intestinal lesions, a combined approach, utilizing endoscopic examination, lesion-directed brushings and biopsy specimens, is required.", "contents": "Diagnosis of upper gastro-intestinal lesions by endoscopy, cytology and biopsy. A prospective consecutive series of 210 upper gastrointestinal endoscopic procedures, at which material for both cytological and histopathological examination was obtained, was conducted at Baragwanath Hospital. The purpose of the study was to determine the respective and combined diagnostic value of endoscopic visualization, cytological brushings and histological sections in benign and malignant lesions of the oesophagus and stomach; and in particular to assess the contribution, if any, of exfoliative cytology to the diagnostic regimen. Endoscopic visualization alone was inconclusive or incorrect in 13% of procedures, cytology alone in 9%, and biopsy alone in 12% (the latter 2 figures corrected for adequacy of material submitted). When assessed together, the diagnostic accuracy achieved by all 3 techniques was 99%. The major contribution of cytology was the correct positive diagnosis in 20% of procedures, performed on patients with malignant lesions, whose initial biopsy specimen was inadequate or normal. It is concluded that for maximal diagnostic accuracy of upper gastro-intestinal lesions, a combined approach, utilizing endoscopic examination, lesion-directed brushings and biopsy specimens, is required."}
{"id": "PMID:462274", "title": "Infective endocarditis at the Johannesburg Hospital. A retrospective analysis of 40 patients.", "content": "The records of 40 patients admitted to the Johannesburg Hospital with a total of 44 episodes of endocarditis were analysed. Pyrexia, albeit often low grade and intermittent, was the most consistent physical sign. The classic symptoms and signs of infective endocarditis were uncommon. Blood cultures were positive in 56% of episodes. However, in 6 out of 9 patients with 'culture-negative' endocarditis, infective endocarditis was confirmed at surgery or necropsy. For haemodynamic reasons, 10 patients (25%) were subjected to valve replacement and 1 to a mitral valvuloplasty. In 2 of these the infection was still active at the time of surgery. Five patients (12,5%) died; in 4 the cause of death was neurological and in the remaining patient the aortic valve ruptured and he died within 30 minutes.", "contents": "Infective endocarditis at the Johannesburg Hospital. A retrospective analysis of 40 patients. The records of 40 patients admitted to the Johannesburg Hospital with a total of 44 episodes of endocarditis were analysed. Pyrexia, albeit often low grade and intermittent, was the most consistent physical sign. The classic symptoms and signs of infective endocarditis were uncommon. Blood cultures were positive in 56% of episodes. However, in 6 out of 9 patients with 'culture-negative' endocarditis, infective endocarditis was confirmed at surgery or necropsy. For haemodynamic reasons, 10 patients (25%) were subjected to valve replacement and 1 to a mitral valvuloplasty. In 2 of these the infection was still active at the time of surgery. Five patients (12,5%) died; in 4 the cause of death was neurological and in the remaining patient the aortic valve ruptured and he died within 30 minutes."}
{"id": "PMID:462276", "title": "[The effect of rooibos tea on iron absorption].", "content": "A study was carried out to determine if rooibos tea (Aspalathus linearis) has a deleterious effect on iron absorption similar to that of ordinary tea (Camellia sinensis). Three groups of volunteers, each consisting of 10 healthy young men comparable with regard to iron status and body dimensions, were studied. After ingestion of 1 muCi 59Fe and 16 mg of elemental iron, group A drank rooibos tea, group B ordinary tea and group C boiled water. Iron absorption was measured 14 days later with a whole body counter. Mean iron absorption after ingestion of rooibos tea, ordinary tea, and water was 7,25%, 1,70% and 9,34% respectively. In contrast to ordinary tea (P less than 0,0001), rooibos tea did not affect iron absorption significantly. The ascorbic acid content of rooibos tea varies between 121,8 and 154,9 mumol/l, depending on the method of preparation. Rooibos tea contains small amounts of iron, magnesium, phosphorus, sodium, chloride and potassium. These findings have practical nutritional and therapeutic implications.", "contents": "[The effect of rooibos tea on iron absorption]. A study was carried out to determine if rooibos tea (Aspalathus linearis) has a deleterious effect on iron absorption similar to that of ordinary tea (Camellia sinensis). Three groups of volunteers, each consisting of 10 healthy young men comparable with regard to iron status and body dimensions, were studied. After ingestion of 1 muCi 59Fe and 16 mg of elemental iron, group A drank rooibos tea, group B ordinary tea and group C boiled water. Iron absorption was measured 14 days later with a whole body counter. Mean iron absorption after ingestion of rooibos tea, ordinary tea, and water was 7,25%, 1,70% and 9,34% respectively. In contrast to ordinary tea (P less than 0,0001), rooibos tea did not affect iron absorption significantly. The ascorbic acid content of rooibos tea varies between 121,8 and 154,9 mumol/l, depending on the method of preparation. Rooibos tea contains small amounts of iron, magnesium, phosphorus, sodium, chloride and potassium. These findings have practical nutritional and therapeutic implications."}
{"id": "PMID:462277", "title": "[Muscle injuries].", "content": "A short summary of the most common muscle injuries is given, as well as a classification into three grades with the corresponding treatment. The common problem of shin splints is dealt with, and a possible explanation of the pathology and the treatment thereof is given.", "contents": "[Muscle injuries]. A short summary of the most common muscle injuries is given, as well as a classification into three grades with the corresponding treatment. The common problem of shin splints is dealt with, and a possible explanation of the pathology and the treatment thereof is given."}
{"id": "PMID:462278", "title": "Haematological differences in three population groups.", "content": "The increased absolute lymphocyte counts of Indians and Blacks in comparison with Whites, observed in a former study, were shown, in a study on blood donors, to occur in women rather than men of these two groups. Neutrophil counts showed no sex differences, but were significantly highest in Whites and lowest in Blacks. Indians of both sexes had significantly higher mean red cell counts, together with smaller red cells, than subjects in the other two groups. In no group was a significant correlation observed between body mass and haemoglobin level.", "contents": "Haematological differences in three population groups. The increased absolute lymphocyte counts of Indians and Blacks in comparison with Whites, observed in a former study, were shown, in a study on blood donors, to occur in women rather than men of these two groups. Neutrophil counts showed no sex differences, but were significantly highest in Whites and lowest in Blacks. Indians of both sexes had significantly higher mean red cell counts, together with smaller red cells, than subjects in the other two groups. In no group was a significant correlation observed between body mass and haemoglobin level."}
{"id": "PMID:462279", "title": "Cockroaches in relation to bronchial asthma in the Durban area.", "content": "Thirty patients from the White and Coloured asthma clinics were subjected to skin prick tests with various antigens. Although the house-dust mite was found to be the main offender, 9 of the 30 patients showed a significant reaction to cockroach antigen. It is felt that cockroaches play a role in the pathogenesis of asthma in the Durban area and that this fact should be considered in desensitization programmes.", "contents": "Cockroaches in relation to bronchial asthma in the Durban area. Thirty patients from the White and Coloured asthma clinics were subjected to skin prick tests with various antigens. Although the house-dust mite was found to be the main offender, 9 of the 30 patients showed a significant reaction to cockroach antigen. It is felt that cockroaches play a role in the pathogenesis of asthma in the Durban area and that this fact should be considered in desensitization programmes."}
{"id": "PMID:462280", "title": "Systemic histoplasmosis with oesophageal obstruction due to Histoplasma granulomas. Successful treatment with rifampicin and amphotericin B.", "content": "A patient with oesophageal stenosis caused by Histoplasma granulomas is reported. He was treated with an initial combined course of intravenous amphotericin B and oral rifampicin. Complications included adrenal insufficiency, operative perforation of the oesophagus, amphotericin nephrotoxicity and tuberculosis. The histoplasmosis has not recurred for over 3 years.", "contents": "Systemic histoplasmosis with oesophageal obstruction due to Histoplasma granulomas. Successful treatment with rifampicin and amphotericin B. A patient with oesophageal stenosis caused by Histoplasma granulomas is reported. He was treated with an initial combined course of intravenous amphotericin B and oral rifampicin. Complications included adrenal insufficiency, operative perforation of the oesophagus, amphotericin nephrotoxicity and tuberculosis. The histoplasmosis has not recurred for over 3 years."}
{"id": "PMID:462281", "title": "Acute confusional states and depression treated with maprotiline mesylate. Report of 4 patients successfully treated in an intensive care unit.", "content": "Four patients admitted to hospital with multiple injuries developed psychiatric symptoms after an initially favourable response to intensive therapy. Alterations in the level of consciousness and behavioural pattern were observed, associated with the presence of acute depression, possibly endogenous in origin. Within 24--48 hours of intravenous administration of maprotiline mesylate (Ludiomil, Ciba), a tetracyclic antidepressant drug, considerable improvement was noted in all 4 patients, with regard to both the depressive state and the clouding of consciousness. The importance of recognizing psychiatric disorders in severely ill patients in an intensive therapy environment is stressed.", "contents": "Acute confusional states and depression treated with maprotiline mesylate. Report of 4 patients successfully treated in an intensive care unit. Four patients admitted to hospital with multiple injuries developed psychiatric symptoms after an initially favourable response to intensive therapy. Alterations in the level of consciousness and behavioural pattern were observed, associated with the presence of acute depression, possibly endogenous in origin. Within 24--48 hours of intravenous administration of maprotiline mesylate (Ludiomil, Ciba), a tetracyclic antidepressant drug, considerable improvement was noted in all 4 patients, with regard to both the depressive state and the clouding of consciousness. The importance of recognizing psychiatric disorders in severely ill patients in an intensive therapy environment is stressed."}
{"id": "PMID:462282", "title": "Overwhelming pneumococcaemia 17 years after splenectomy. A case report.", "content": "We present a case of fulminant pneumococcaemia and disseminated intravascular coagulopathy in a young adult man 17 years after splenectomy. The clinical presentation, laboratory and postmortem findings are discussed. The diagnosis, management and prophylaxis of overwhelming infections in splenectomized patients are reviewed. The advent of pneumococcal and other vaccines could contribute significantly to the successful protection of asplenic patients against certain severe infections.", "contents": "Overwhelming pneumococcaemia 17 years after splenectomy. A case report. We present a case of fulminant pneumococcaemia and disseminated intravascular coagulopathy in a young adult man 17 years after splenectomy. The clinical presentation, laboratory and postmortem findings are discussed. The diagnosis, management and prophylaxis of overwhelming infections in splenectomized patients are reviewed. The advent of pneumococcal and other vaccines could contribute significantly to the successful protection of asplenic patients against certain severe infections."}
{"id": "PMID:462288", "title": "Osteopetrosis in South Africa. The benign, lethal and intermediate forms.", "content": "Osteopetrosis is an unusual bone disorder in which the skeleton is radiographically dense. The condition is conventionally subclassified into a benign autosomal dominant adult form and a malignant autosomal recessive variety. Among 14 affected individuals whom we have studied, 4 adults had an intermediate type of osteopetrosis in which serious complications included osteomyelitis, pathological fractures and dyshaemopoiesis. The fundamental biochemical relationship of this disorder with the classic forms of osteopetrosis is uncertain. The osteopetroses must be distinguished from other sclerosing bone conditions which have a different course and prognosis. Sclerosteosis and craniometaphyseal dysplasia, both of which occur in South Africa, are of practical importance in this context.", "contents": "Osteopetrosis in South Africa. The benign, lethal and intermediate forms. Osteopetrosis is an unusual bone disorder in which the skeleton is radiographically dense. The condition is conventionally subclassified into a benign autosomal dominant adult form and a malignant autosomal recessive variety. Among 14 affected individuals whom we have studied, 4 adults had an intermediate type of osteopetrosis in which serious complications included osteomyelitis, pathological fractures and dyshaemopoiesis. The fundamental biochemical relationship of this disorder with the classic forms of osteopetrosis is uncertain. The osteopetroses must be distinguished from other sclerosing bone conditions which have a different course and prognosis. Sclerosteosis and craniometaphyseal dysplasia, both of which occur in South Africa, are of practical importance in this context."}
{"id": "PMID:462289", "title": "Prolonged atrioventricular conduction in young children and adolescents. The role of increased vagal tone.", "content": "Prolonged atrioventricular (A-V) conduction is often an apparently normal finding in children with or without congenital heart disease. The mechanism is probably vagally mediated and appears to be benign in the majority of cases. Eleven children with first-degree and/or second-degree Mobitz type 1 A-V block were studied by intracardiac His bundle electrocardiography with atrial pacing. Six children had congenital heart disease with left-to-right shunts and 5 had normal cardiac anatomy. In all the patients studied, the A-H intervals were longer than normal. Wenckebach periodicity appeared at relatively long cycle lengths, and in 5 of the 6 patients in whom values could be determined, atrioventriculonodal effective and functional refractory periods were prolonged. With atropine and isoproterenol conduction intervals returned to normal. There was no tendency to develop echo beats with atrial extrastimuli, even when A-H intervals became markedly prolonged. The results of this study suggest that vagal tone plays a significant role in the prolongation of A-V conduction, even in congenital heart lesions that are known to be associated with a long PR interval.", "contents": "Prolonged atrioventricular conduction in young children and adolescents. The role of increased vagal tone. Prolonged atrioventricular (A-V) conduction is often an apparently normal finding in children with or without congenital heart disease. The mechanism is probably vagally mediated and appears to be benign in the majority of cases. Eleven children with first-degree and/or second-degree Mobitz type 1 A-V block were studied by intracardiac His bundle electrocardiography with atrial pacing. Six children had congenital heart disease with left-to-right shunts and 5 had normal cardiac anatomy. In all the patients studied, the A-H intervals were longer than normal. Wenckebach periodicity appeared at relatively long cycle lengths, and in 5 of the 6 patients in whom values could be determined, atrioventriculonodal effective and functional refractory periods were prolonged. With atropine and isoproterenol conduction intervals returned to normal. There was no tendency to develop echo beats with atrial extrastimuli, even when A-H intervals became markedly prolonged. The results of this study suggest that vagal tone plays a significant role in the prolongation of A-V conduction, even in congenital heart lesions that are known to be associated with a long PR interval."}
{"id": "PMID:462291", "title": "A community psychiatric programme for Soweto. Report on a pilot study.", "content": "A pilot study was done on 50 patients in a community psychiatric programme in Soweto. The organization was based on the principles of mental health consultation and the treatment was based on primary, secondary and tertiary prevention. The role of the multidisciplinary staff is outlined and the role of maintenance pharmacotherapy for the ex-schizophrenic patient is stressed. The efficacy of the programme could be improved by an increase in the staff-patient ratio.", "contents": "A community psychiatric programme for Soweto. Report on a pilot study. A pilot study was done on 50 patients in a community psychiatric programme in Soweto. The organization was based on the principles of mental health consultation and the treatment was based on primary, secondary and tertiary prevention. The role of the multidisciplinary staff is outlined and the role of maintenance pharmacotherapy for the ex-schizophrenic patient is stressed. The efficacy of the programme could be improved by an increase in the staff-patient ratio."}
{"id": "PMID:462301", "title": "Factors involved in the acceptance of voluntary female sterilization among Indians in Natal.", "content": "The sociological characteristics of 200 Indian women admitted for voluntary sterilization to King George V and R. K. Khan Hospitals, Durban, were investigated. Factors involved in the decision to be sterilized were analysed, as well as socio-economic motives found to be of major significance in the desire to limit family size. A control group of 200 Indian women, none of whom had been sterilized, was interviewed, and responses revealed a very favourable attitude towards the acceptance of female sterilization once the family is complete.", "contents": "Factors involved in the acceptance of voluntary female sterilization among Indians in Natal. The sociological characteristics of 200 Indian women admitted for voluntary sterilization to King George V and R. K. Khan Hospitals, Durban, were investigated. Factors involved in the decision to be sterilized were analysed, as well as socio-economic motives found to be of major significance in the desire to limit family size. A control group of 200 Indian women, none of whom had been sterilized, was interviewed, and responses revealed a very favourable attitude towards the acceptance of female sterilization once the family is complete."}
{"id": "PMID:462302", "title": "A new universal anaesthetic circuit using a preferential-flow T-piece.", "content": "A new co-axial anaesthetic circuit, employing a preferential-flow T-piece which eliminates the need for valves in the circuit, has been designed. Its efficiency has been shown to compare favourably with that of conventional circuits for use in spontaneous respiration and under conditions of controlled ventilation. The proposed system has the advantage of convenience, in that there is no need to disconnect the system from the patient nor from the anaesthetic machine when it is necessary to change from spontaneous to controlled ventilation or should a circle absorber be incorporated within the circuit. The system has been shown to offer lower resistance than the Mapleson A circuit.", "contents": "A new universal anaesthetic circuit using a preferential-flow T-piece. A new co-axial anaesthetic circuit, employing a preferential-flow T-piece which eliminates the need for valves in the circuit, has been designed. Its efficiency has been shown to compare favourably with that of conventional circuits for use in spontaneous respiration and under conditions of controlled ventilation. The proposed system has the advantage of convenience, in that there is no need to disconnect the system from the patient nor from the anaesthetic machine when it is necessary to change from spontaneous to controlled ventilation or should a circle absorber be incorporated within the circuit. The system has been shown to offer lower resistance than the Mapleson A circuit."}
{"id": "PMID:462303", "title": "Two simple inexpensive photographic methods for viewing ECG-gated radionuclide blood pool images.", "content": "Although the ECG-gated radionuclide blood pool scan (GBPS) has become an established method for studying regional myocardial wall motion, it is usually performed with the aid of an expensive computer system. A simple, inexpensive method was developed to view gated radionuclide blood images by a film loop and a photographic motion detection (PHOMOT) technique. These techniques were compared with left ventricular cine angiography in 15 patients. Segmental wall movement (78 segments) showed identical results in 92% of cases. In all patients the same diagnosis was arrived at by GBPS and cine angiography. The photographic techniques developed offer a simple screening procedure to reduce cardiac catheterization in patients with suspected abnormalities of left ventricular wall contraction.", "contents": "Two simple inexpensive photographic methods for viewing ECG-gated radionuclide blood pool images. Although the ECG-gated radionuclide blood pool scan (GBPS) has become an established method for studying regional myocardial wall motion, it is usually performed with the aid of an expensive computer system. A simple, inexpensive method was developed to view gated radionuclide blood images by a film loop and a photographic motion detection (PHOMOT) technique. These techniques were compared with left ventricular cine angiography in 15 patients. Segmental wall movement (78 segments) showed identical results in 92% of cases. In all patients the same diagnosis was arrived at by GBPS and cine angiography. The photographic techniques developed offer a simple screening procedure to reduce cardiac catheterization in patients with suspected abnormalities of left ventricular wall contraction."}
{"id": "PMID:462311", "title": "The first published chemical analyses of smoke from South African cigarettes.", "content": "The smoke of 69 brands of cigarettes sold in South Africa in March 1978 was analysed for its content of tar, nicotine, carbon monoxide and carbon dioxide. The average yield of tar per cigarette varied between 30 and 38,5 mg in 27 brands and between 20 and 30 mg in all but one of the other 42 brands. The nicotine yields with one exception varied between 1,0 and 2,40 mg per cigarette and generally correlated well with those of tar (r = 0,75). The yields of carbon monoxide per cigarette generally ranged from about 14 to 32 mg, and those of carbon dioxide from about 50 to 85 mg. The results of this analysis, the first to be published with regard to South African cigarettes, are very disturbing. In particular, the tar yields are unacceptably high by international standards. They demand urgent action including (a) regular cigarette analyses by the Government, and publication of the results by the mass media, especially the SABC; (b) compulsory printing of the results of analyses on every cigarette pack and advertisement; (c) education of the public with regard to the meaning of the analyses; and (d) a programme designed to replace more dangerous cigarettes with less hazardous ones. Most important, all these measures must be implemented within the framework of a really meaningful, systematic, continuous and appropriate campaign aimed at combating the tobacco hazard at every level in all population groups.", "contents": "The first published chemical analyses of smoke from South African cigarettes. The smoke of 69 brands of cigarettes sold in South Africa in March 1978 was analysed for its content of tar, nicotine, carbon monoxide and carbon dioxide. The average yield of tar per cigarette varied between 30 and 38,5 mg in 27 brands and between 20 and 30 mg in all but one of the other 42 brands. The nicotine yields with one exception varied between 1,0 and 2,40 mg per cigarette and generally correlated well with those of tar (r = 0,75). The yields of carbon monoxide per cigarette generally ranged from about 14 to 32 mg, and those of carbon dioxide from about 50 to 85 mg. The results of this analysis, the first to be published with regard to South African cigarettes, are very disturbing. In particular, the tar yields are unacceptably high by international standards. They demand urgent action including (a) regular cigarette analyses by the Government, and publication of the results by the mass media, especially the SABC; (b) compulsory printing of the results of analyses on every cigarette pack and advertisement; (c) education of the public with regard to the meaning of the analyses; and (d) a programme designed to replace more dangerous cigarettes with less hazardous ones. Most important, all these measures must be implemented within the framework of a really meaningful, systematic, continuous and appropriate campaign aimed at combating the tobacco hazard at every level in all population groups."}
{"id": "PMID:462312", "title": "Progesterone in obese patients with respiratory failure.", "content": "Two obese patients with acute respiratory failure are described. Both required mechanical ventilation to reverse the respiratory acidosis. On recovery, the major abnormality was failure of ventilatory response to inhaled carbon dioxide. After oral progesterone (100 mg/day and 20 mg/day, respectively) the slope of the carbon dioxide response line increased from 0,02 to 1,14 I min-1 mmHg-1 and from 0,04 to 1,14 I min-1 mmHg-1, respectively. This was associated with sustained clinical remission in 1 patient.", "contents": "Progesterone in obese patients with respiratory failure. Two obese patients with acute respiratory failure are described. Both required mechanical ventilation to reverse the respiratory acidosis. On recovery, the major abnormality was failure of ventilatory response to inhaled carbon dioxide. After oral progesterone (100 mg/day and 20 mg/day, respectively) the slope of the carbon dioxide response line increased from 0,02 to 1,14 I min-1 mmHg-1 and from 0,04 to 1,14 I min-1 mmHg-1, respectively. This was associated with sustained clinical remission in 1 patient."}
{"id": "PMID:462313", "title": "Intestinal parasitic infestation in urban and rural Xhosa children. A comparative study.", "content": "Evidence of helminthic intestinal parasites was found in 97,0% of stools of 544 Xhosa schoolchildren living in Cape Town. The commonest parasite was Trichuris trichiura, which infested 89,9% of the children. In contrast, only 9,8% of 387 Xhosa children living in the Tsolo district, Transkei, had evidence of intestinal parasites in their stools, Hymenolepis nana being the commonest. In spite of the unsatisfactory sanitary conditions in the Tsolo area compared with those in Guguletu, Cape Town, where all sewage is waterborne, few rural children were infested. The high prevalence of infestation in the Guguletu children suggests extensive contamination of the soil in the area by parasitic ova. In view of the magnitude of the extent of parasitic infestation in the Guguletu children, a vigorous and sustained programme of medication and education will have to be undertaken if the problem is to be resolved.", "contents": "Intestinal parasitic infestation in urban and rural Xhosa children. A comparative study. Evidence of helminthic intestinal parasites was found in 97,0% of stools of 544 Xhosa schoolchildren living in Cape Town. The commonest parasite was Trichuris trichiura, which infested 89,9% of the children. In contrast, only 9,8% of 387 Xhosa children living in the Tsolo district, Transkei, had evidence of intestinal parasites in their stools, Hymenolepis nana being the commonest. In spite of the unsatisfactory sanitary conditions in the Tsolo area compared with those in Guguletu, Cape Town, where all sewage is waterborne, few rural children were infested. The high prevalence of infestation in the Guguletu children suggests extensive contamination of the soil in the area by parasitic ova. In view of the magnitude of the extent of parasitic infestation in the Guguletu children, a vigorous and sustained programme of medication and education will have to be undertaken if the problem is to be resolved."}
{"id": "PMID:462314", "title": "Serum ferritin concentrations in black miners.", "content": "Serum ferritin concentrations were measured in 651 Black male miners who originated from rural areas throughout southern Africa and who were aged between 17 and 57 years. The mean serum ferritin concentration of 229 microgram/l was above the normal range reported for White subjects, and in 52,8% of the subjects the values were greater than 200 microgram/l. The serum ferritin concentration rose with age, as did the proportion of subjects in each age group who exhibited high values (more than 200 microgram/l). The lowest mean ferritin concentration (112 microgram/l) as well as the lowest proportion of high values (22,9%) were found in subjects from the most northerly area studied. Similarly, the highest mean proportion of high values (66,3%) was seen in the most southerly group studied. Calculations from the present data suggest that the degree of iron overload is currently greater in rural than in urban Black male subjects.", "contents": "Serum ferritin concentrations in black miners. Serum ferritin concentrations were measured in 651 Black male miners who originated from rural areas throughout southern Africa and who were aged between 17 and 57 years. The mean serum ferritin concentration of 229 microgram/l was above the normal range reported for White subjects, and in 52,8% of the subjects the values were greater than 200 microgram/l. The serum ferritin concentration rose with age, as did the proportion of subjects in each age group who exhibited high values (more than 200 microgram/l). The lowest mean ferritin concentration (112 microgram/l) as well as the lowest proportion of high values (22,9%) were found in subjects from the most northerly area studied. Similarly, the highest mean proportion of high values (66,3%) was seen in the most southerly group studied. Calculations from the present data suggest that the degree of iron overload is currently greater in rural than in urban Black male subjects."}
{"id": "PMID:462315", "title": "Women doctors wasted.", "content": "The problem of wastage of women doctors has exercised the minds of South African medical planners very little. Numerous articles have appeared in the English literature, but we have been able to find only two editorials in semi-facetious vein in the South African Medical Journal over the last 20 years. We have looked at some trends, and believe that there is a need for further investigation, because there are important implications concerning future medical manpower resources in this country.", "contents": "Women doctors wasted. The problem of wastage of women doctors has exercised the minds of South African medical planners very little. Numerous articles have appeared in the English literature, but we have been able to find only two editorials in semi-facetious vein in the South African Medical Journal over the last 20 years. We have looked at some trends, and believe that there is a need for further investigation, because there are important implications concerning future medical manpower resources in this country."}
{"id": "PMID:462316", "title": "Endoscopic retrograde cholangiopancreatography in the management of traumatic pancreatic pseudocysts. A report of 2 cases.", "content": "The results of endoscopic retrograde cholangiopancreatography (ERCP) in 2 patients with traumatic pancreatic pseudocysts are described. As a pre-operative procedure, this investigation provided useful information on the exact site of duct disruption. In both patients, the pancreatic pseudocysts were drained via a posterior cyst gastrostomy, and they have remained well since surgery. Follow-up ERCP at 6 and 12 months demonstrated complete stenosis at the site of duct disruption. The value of ERCP in the pre-operative and follow-up management of traumatic pancreatic pseudocysts is discussed.", "contents": "Endoscopic retrograde cholangiopancreatography in the management of traumatic pancreatic pseudocysts. A report of 2 cases. The results of endoscopic retrograde cholangiopancreatography (ERCP) in 2 patients with traumatic pancreatic pseudocysts are described. As a pre-operative procedure, this investigation provided useful information on the exact site of duct disruption. In both patients, the pancreatic pseudocysts were drained via a posterior cyst gastrostomy, and they have remained well since surgery. Follow-up ERCP at 6 and 12 months demonstrated complete stenosis at the site of duct disruption. The value of ERCP in the pre-operative and follow-up management of traumatic pancreatic pseudocysts is discussed."}
{"id": "PMID:462325", "title": "The recognition of lesions on chest radiographs.", "content": "An approach to the recognition of lesions on chest radiographs is outlined. Four basic groups of abnormalities by means of which lesions can be recognized are mentioned and are briefly described, namely an opacity, an area of increased translucency, a combination of an opacity and an area of increased translucency, and, especially, an alteration in normal radiological anatomy.", "contents": "The recognition of lesions on chest radiographs. An approach to the recognition of lesions on chest radiographs is outlined. Four basic groups of abnormalities by means of which lesions can be recognized are mentioned and are briefly described, namely an opacity, an area of increased translucency, a combination of an opacity and an area of increased translucency, and, especially, an alteration in normal radiological anatomy."}
{"id": "PMID:462326", "title": "Radiological features of pulmonary oedema.", "content": "A clinical classification embracing most of the causes of pulmonary oedema is given, as well as a radiological classification, and the different ways in which pulmonary oedema may present radiologically are briefly described.", "contents": "Radiological features of pulmonary oedema. A clinical classification embracing most of the causes of pulmonary oedema is given, as well as a radiological classification, and the different ways in which pulmonary oedema may present radiologically are briefly described."}
{"id": "PMID:462328", "title": "The first symptoms of carcinoma of the oesophagus, with particular reference to amaqhakuva. A report from the Republic of Transkei.", "content": "The first symptoms of cancer of the oesophagus in 82 Xhosa patients were found to be dysphagia (43%), interscapular back pain (23%), sore throat (21%) and epigastric pain (11%). More than half of the patients in whom a sore throat was the first symptom of the disease described the simultaneous occurrence of oropharyngeal lesions (amaqhakuva). No correlation could be found between the first symptoms and the characteristics of the oesophageal carcinoma at the time of presentation.", "contents": "The first symptoms of carcinoma of the oesophagus, with particular reference to amaqhakuva. A report from the Republic of Transkei. The first symptoms of cancer of the oesophagus in 82 Xhosa patients were found to be dysphagia (43%), interscapular back pain (23%), sore throat (21%) and epigastric pain (11%). More than half of the patients in whom a sore throat was the first symptom of the disease described the simultaneous occurrence of oropharyngeal lesions (amaqhakuva). No correlation could be found between the first symptoms and the characteristics of the oesophageal carcinoma at the time of presentation."}
{"id": "PMID:462329", "title": "The comparative efficacy and safety of aminoglycoside antibiotics.", "content": "In this article a report submitted to the Director of Hospital Services of the Cape Provincial Administration is summarized. The object of the report was to make firm recommendations where possible as to the use of the aminoglycosides in the Cape hospitals, taking into account considerations of comparative efficacy, safety and cost.", "contents": "The comparative efficacy and safety of aminoglycoside antibiotics. In this article a report submitted to the Director of Hospital Services of the Cape Provincial Administration is summarized. The object of the report was to make firm recommendations where possible as to the use of the aminoglycosides in the Cape hospitals, taking into account considerations of comparative efficacy, safety and cost."}
{"id": "PMID:462330", "title": "Incidence of heart disease in pregnancy. A study done at Peninsula Maternity Services hospitals.", "content": "A detailed retrospective analysis was made of the records of 697 women with heart disease who were delivered between 1972 and 1976 and who accounted for 0.83% of all deliveries during this time. Rheumatic heart disease accounted for 65%, congenital lesions for 14% patients who had had cardiac surgery 12%, and miscellaneous heart conditions for 10% of cardiac lesions. The maternal mortality rate in these women was 7,17/1,000 deliveries, compared with 0.46/1,000 deliveries during this time. Cardiac disease was found to be the most important non-obstetric cause of maternal death. The perinatal mortality rate was not significantly higher than that in the total population. The high incidence of cardiac disease in pregnancy is unlikely to be significantly reduced until effective contraception is more widely practised in our community.", "contents": "Incidence of heart disease in pregnancy. A study done at Peninsula Maternity Services hospitals. A detailed retrospective analysis was made of the records of 697 women with heart disease who were delivered between 1972 and 1976 and who accounted for 0.83% of all deliveries during this time. Rheumatic heart disease accounted for 65%, congenital lesions for 14% patients who had had cardiac surgery 12%, and miscellaneous heart conditions for 10% of cardiac lesions. The maternal mortality rate in these women was 7,17/1,000 deliveries, compared with 0.46/1,000 deliveries during this time. Cardiac disease was found to be the most important non-obstetric cause of maternal death. The perinatal mortality rate was not significantly higher than that in the total population. The high incidence of cardiac disease in pregnancy is unlikely to be significantly reduced until effective contraception is more widely practised in our community."}
{"id": "PMID:462338", "title": "Mandating private pensions: experience in four European countries.", "content": "This study examines the experience of four countries--the Netherlands, Sweden, Switzerland, and the United Kingdom--in which the mandating of private pensions exists or has been considered. Proposals to mandate private pensions in the United States have been introduced in Congress several times. The analysis of foreign thinking presented here provides a background on the reasoning behind such a policy and on the integration of private and public systems and the problems involved. A prime reason for mandating private pensions--instead of seeking higher social security benefits or additional social security layers--has been the pressure to avoid higher payroll taxes. Some countries already had such high contribution rates that they sought other means to improve benefits. Adding a layer of private pensions, it was thought, does not involve Government mechanisms and keeps the money in the private sector. Yet mandating by law creates many problems, and no country has fully implemented such legislation.", "contents": "Mandating private pensions: experience in four European countries. This study examines the experience of four countries--the Netherlands, Sweden, Switzerland, and the United Kingdom--in which the mandating of private pensions exists or has been considered. Proposals to mandate private pensions in the United States have been introduced in Congress several times. The analysis of foreign thinking presented here provides a background on the reasoning behind such a policy and on the integration of private and public systems and the problems involved. A prime reason for mandating private pensions--instead of seeking higher social security benefits or additional social security layers--has been the pressure to avoid higher payroll taxes. Some countries already had such high contribution rates that they sought other means to improve benefits. Adding a layer of private pensions, it was thought, does not involve Government mechanisms and keeps the money in the private sector. Yet mandating by law creates many problems, and no country has fully implemented such legislation."}
{"id": "PMID:462340", "title": "Effect of substantial gainful activity level on disabled beneficiary work patterns.", "content": "Analysis of disabled beneficiaries' earnings records from the 1-percent Continuous Work History Sample over a 15-year period indicates that increases in the substantial gainful activity (SGA) level in 1966, 1968, and 1974 were not followed by incremental increases in beneficiary earnings. This article examines the flow of disabled-worker beneficiaries through the disability program in relation to their work history and earnings patterns. The recovery rate dropped steadily during the late 1960's and early 1970's. The earnings patterns indicate that about 10 percent of the beneficiaries were employed; about 3 percent had earnings above the SGA level. Median annual earnings remained well below $1,000, whatever the SGA level. Findings of the study suggest that policy considerations on changing the SGA level should not center on the implications for marginal part-time employment. Most of the evidence from beneficiary work and earnings patterns indicates that the SGA level has not operated as an effective work incentive. The program-flow analysis suggests that the allowance process rather than termination of benefits for recovery after sustained work holds the key to controlling program growth.", "contents": "Effect of substantial gainful activity level on disabled beneficiary work patterns. Analysis of disabled beneficiaries' earnings records from the 1-percent Continuous Work History Sample over a 15-year period indicates that increases in the substantial gainful activity (SGA) level in 1966, 1968, and 1974 were not followed by incremental increases in beneficiary earnings. This article examines the flow of disabled-worker beneficiaries through the disability program in relation to their work history and earnings patterns. The recovery rate dropped steadily during the late 1960's and early 1970's. The earnings patterns indicate that about 10 percent of the beneficiaries were employed; about 3 percent had earnings above the SGA level. Median annual earnings remained well below $1,000, whatever the SGA level. Findings of the study suggest that policy considerations on changing the SGA level should not center on the implications for marginal part-time employment. Most of the evidence from beneficiary work and earnings patterns indicates that the SGA level has not operated as an effective work incentive. The program-flow analysis suggests that the allowance process rather than termination of benefits for recovery after sustained work holds the key to controlling program growth."}
{"id": "PMID:462342", "title": "Appeals under the SSI Program: January 1974-August 1976.", "content": "This study of appeals under the supplemental security income (SSI) program has several purposes: (1) To describe the appellate process and provide information on those requesting appeals (including reason for eligibility, program status, and age) and (2) to determine the relationship between these characteristics and the decisions at the three stages of the appellate process. About 312,000 reconsideration requests were processed from January 1974 to August 1976. These requests had been filed by adult and child SSI applicants who disagreed with the initial determinations of the Social Security Administration. Disabled persons requested nearly 95 percent of these reconsiderations. Approximately one-third of the applicants whose initial determination was reaffirmed after reconsideration then requested a hearing. Ninety-seven percent of these requests were filed by disabled persons. For those cases in which the earlier decisions were reaffirmed after hearings, 9,300 applicants requested a review by the Appeals Council. Eighty-two percent of these requests were denied.", "contents": "Appeals under the SSI Program: January 1974-August 1976. This study of appeals under the supplemental security income (SSI) program has several purposes: (1) To describe the appellate process and provide information on those requesting appeals (including reason for eligibility, program status, and age) and (2) to determine the relationship between these characteristics and the decisions at the three stages of the appellate process. About 312,000 reconsideration requests were processed from January 1974 to August 1976. These requests had been filed by adult and child SSI applicants who disagreed with the initial determinations of the Social Security Administration. Disabled persons requested nearly 95 percent of these reconsiderations. Approximately one-third of the applicants whose initial determination was reaffirmed after reconsideration then requested a hearing. Ninety-seven percent of these requests were filed by disabled persons. For those cases in which the earlier decisions were reaffirmed after hearings, 9,300 applicants requested a review by the Appeals Council. Eighty-two percent of these requests were denied."}
{"id": "PMID:462343", "title": "Recovery of disabled beneficiaries: a 1975 followup study of 1972 allowances.", "content": "In recent years, the number of persons awarded disabled-worker benefits has rapidly increased, but no corresponding rise has occurred in the number leaving the rolls for recovery. A comparative analysis has been made of the demographic, disability, and benefit characteristics of a sample of disabled workers awarded benefits in 1972 who left the rolls for recovery and the characteristics of those who remained on the rolls. For most beneficiaries, recovery appeared unlikely. A large proportion of those awarded benefits were older middle-aged workers with chronic progressive diseases. About 7 in 10 were aged 50 or over at allowance; one-fifth had died by the end of 1975. Younger beneficiaries, those with more education, those disabled by injuries, and residents of Western States were most likely to recover. Persons with higher benefit amounts had a high rate of recovery, but this finding appeared to reflect the effects of their being younger and more skilled. The level of earnings replacement appeared to have little independent effect on recovery. Among workers with conditions most subject to medical improvement, however, those with high replacement rates were less likely to leave the rolls.", "contents": "Recovery of disabled beneficiaries: a 1975 followup study of 1972 allowances. In recent years, the number of persons awarded disabled-worker benefits has rapidly increased, but no corresponding rise has occurred in the number leaving the rolls for recovery. A comparative analysis has been made of the demographic, disability, and benefit characteristics of a sample of disabled workers awarded benefits in 1972 who left the rolls for recovery and the characteristics of those who remained on the rolls. For most beneficiaries, recovery appeared unlikely. A large proportion of those awarded benefits were older middle-aged workers with chronic progressive diseases. About 7 in 10 were aged 50 or over at allowance; one-fifth had died by the end of 1975. Younger beneficiaries, those with more education, those disabled by injuries, and residents of Western States were most likely to recover. Persons with higher benefit amounts had a high rate of recovery, but this finding appeared to reflect the effects of their being younger and more skilled. The level of earnings replacement appeared to have little independent effect on recovery. Among workers with conditions most subject to medical improvement, however, those with high replacement rates were less likely to leave the rolls."}
{"id": "PMID:462346", "title": "Dagnostic imaging of the upper part of the abdomen.", "content": "The new diagnostic procedures developed during the last several years present a confusing dilemma. The state of the art of ultrasound, computed tomography and nuclear scintigraphy is progressing so rapidly that what one says today is generally out of date tomorrow. However, certain guidelines should be followed in evaluating various organ systems. Generally, ultrasound is the procedure of choice in the upper part of the abdomen in most patients who are not extremely obese or filled with intestinal gas. A sonogram is less expensive than computed tomography and, at the present time, is considered noninvasive compared with compound tomography, which produces a small, but significant, radiation exposure. The accuracy of the two modalities is quite similar. Using the slight advantage that computed tomography may have in a given organ system does not generally override other factors, such as cost and radiation dose. However, mitigating factors, such as obesity, presence or absence of intestinal gas or barium, should be taken into account when selecting patients for either procedure. In many clinical situations, neither computed tomography nor ultrasound should be used until other modalities, such as conventional radiography or scintigraphy, have first been tried. Should the problem not be resolved by these more conventional techniques, an ultrasound or computer tomographic examination may be indicated to delineate further the pathologic condition of the abdomen.", "contents": "Dagnostic imaging of the upper part of the abdomen. The new diagnostic procedures developed during the last several years present a confusing dilemma. The state of the art of ultrasound, computed tomography and nuclear scintigraphy is progressing so rapidly that what one says today is generally out of date tomorrow. However, certain guidelines should be followed in evaluating various organ systems. Generally, ultrasound is the procedure of choice in the upper part of the abdomen in most patients who are not extremely obese or filled with intestinal gas. A sonogram is less expensive than computed tomography and, at the present time, is considered noninvasive compared with compound tomography, which produces a small, but significant, radiation exposure. The accuracy of the two modalities is quite similar. Using the slight advantage that computed tomography may have in a given organ system does not generally override other factors, such as cost and radiation dose. However, mitigating factors, such as obesity, presence or absence of intestinal gas or barium, should be taken into account when selecting patients for either procedure. In many clinical situations, neither computed tomography nor ultrasound should be used until other modalities, such as conventional radiography or scintigraphy, have first been tried. Should the problem not be resolved by these more conventional techniques, an ultrasound or computer tomographic examination may be indicated to delineate further the pathologic condition of the abdomen."}
{"id": "PMID:462347", "title": "Effect of Corynebacterium parvum in prevention and reversal of atrophy of the liver following portacaval shunt.", "content": "Since it has been demonstrated that the administration of Corynebacterium parvum is associated with increased hepatocyte proliferation in both normal and regenerating livers, it seemed appropriate to determine whether the use of this agent would affect the hepatic atrophy which occurs following end-to-side portacaval shunts. When administered at shunting, liver weight and liver deoxyribonucleic acid failed to decrease to the same extent as that occurring in untreated, shunted rats. An increase in liver deoxyribonucleic acid synthesis was observed following portacaval shunt only in treated rats. Corynebacterium parvum administration to portacaval shunt rats with livers demonstrating marked atrophy 15 to 18 days following shunting resulted in the reversal of such atrophy. There was an increase in liver weight and deoxyribonuclei acid to the extent that those values were equal to, or greater than, those of livers from nonshunted rats. By increasing the number of cells within liver, Corynebacterium parvum administration prevents or reverses the decrease in liver size, that is, atrophy, which occurs after portacaval shunts were performed.", "contents": "Effect of Corynebacterium parvum in prevention and reversal of atrophy of the liver following portacaval shunt. Since it has been demonstrated that the administration of Corynebacterium parvum is associated with increased hepatocyte proliferation in both normal and regenerating livers, it seemed appropriate to determine whether the use of this agent would affect the hepatic atrophy which occurs following end-to-side portacaval shunts. When administered at shunting, liver weight and liver deoxyribonucleic acid failed to decrease to the same extent as that occurring in untreated, shunted rats. An increase in liver deoxyribonucleic acid synthesis was observed following portacaval shunt only in treated rats. Corynebacterium parvum administration to portacaval shunt rats with livers demonstrating marked atrophy 15 to 18 days following shunting resulted in the reversal of such atrophy. There was an increase in liver weight and deoxyribonuclei acid to the extent that those values were equal to, or greater than, those of livers from nonshunted rats. By increasing the number of cells within liver, Corynebacterium parvum administration prevents or reverses the decrease in liver size, that is, atrophy, which occurs after portacaval shunts were performed."}
{"id": "PMID:462348", "title": "Hyperammonemia following ureterocolostomy in the rat.", "content": "Following ureterosigmoidostomy, encephalopathy with hyperammonemia may occur in the presence of cirrhosis, and the same complication was also observed in a few patients without liver damage. This suggests overloading of normal liver ureagenisis by an increased portal ammonia supply. To test this hypothesis and to try to produce an experimental model of chronic hyperammonemia without portal or hepatic damage, ureterocolostomies were performed in rats. These rats were compared with sham operated upon rats and with rats having chronic uremia induced by subtotal nephrectomy. Rats having a ureterocolostomy had chronic, but moderate, systemic hyperammonemia without any histologic hepatic damage and without gross behavioral modifications and slight uremia with only inconstant pyelonephretic lesions. In these rats, hyperammonemia results from hepatic overloading by the increased portal ammonia supply which is a consequence of both intestinal absorption of some urinary ammonia and increased intestinal ammoniagenesis induced by hydrolysis of urinary and circulating urea.", "contents": "Hyperammonemia following ureterocolostomy in the rat. Following ureterosigmoidostomy, encephalopathy with hyperammonemia may occur in the presence of cirrhosis, and the same complication was also observed in a few patients without liver damage. This suggests overloading of normal liver ureagenisis by an increased portal ammonia supply. To test this hypothesis and to try to produce an experimental model of chronic hyperammonemia without portal or hepatic damage, ureterocolostomies were performed in rats. These rats were compared with sham operated upon rats and with rats having chronic uremia induced by subtotal nephrectomy. Rats having a ureterocolostomy had chronic, but moderate, systemic hyperammonemia without any histologic hepatic damage and without gross behavioral modifications and slight uremia with only inconstant pyelonephretic lesions. In these rats, hyperammonemia results from hepatic overloading by the increased portal ammonia supply which is a consequence of both intestinal absorption of some urinary ammonia and increased intestinal ammoniagenesis induced by hydrolysis of urinary and circulating urea."}
{"id": "PMID:462349", "title": "Clinical implications in bacteroides bacteremia.", "content": "The clinical presentation and results of treatment of 98 patients with documented bacteroides bacteremia have been reviewed. Surgically treatable causes were documented in three-quarters of the patients. No one antibiotic or combination thereof significantly influenced survival. Patterns of death from sepsis were through multiple organ failure. Early recognition, or surgical drainage or debridement, or both, and specific aerobe directed antibiotic therapy remain the first line of therapeutic endeavor.", "contents": "Clinical implications in bacteroides bacteremia. The clinical presentation and results of treatment of 98 patients with documented bacteroides bacteremia have been reviewed. Surgically treatable causes were documented in three-quarters of the patients. No one antibiotic or combination thereof significantly influenced survival. Patterns of death from sepsis were through multiple organ failure. Early recognition, or surgical drainage or debridement, or both, and specific aerobe directed antibiotic therapy remain the first line of therapeutic endeavor."}
{"id": "PMID:462350", "title": "Relationship of burn size to vascular permeability changes in rats.", "content": "Studies to determine the relationship between increased vascular permeability, water content and protein leakage, and burn size were performed on rats. Partial thickness scald burn covering 10, 20, 30 and 40 per cent of the rats' body surface area were induced in four groups of rats. A fifth group of rats served as the controls. Following the burn, all rats received intravenously fluid resuscitation. Burn edema was maximal at three hours postburn for the 30 and 40 per cent groups. The edema persisted throughout the 24 hour study period. No significant change in water content of unburned skin was demonstrated. Albumin leakage into injuried tissues was maximal at 30 minutes and disappeared by 12 hours postinjury. The size of the burn had no effect on 131Risa leakage into injuried tissues. Albumin leakage into noninjured tissues occurred only in the 30 and 40 per cent burn groups was mild compared with that in burned tissues and also disappeared by 12 hours postburn. Extravasation of albumin in lung, liver, spleen, kidney and muscle was mild and seen only in the 30 and 40 per cent burn groups. Except for a mild increase in water content of lung tissue in the 30 and 40 per cent groups only, no consistent increase in the water content of other organs was observed. While the size of the burn appears to have some influence on capillary permeability, burns up to 40 per cent of the body surface have only a mild and transient effect upon capillaries distant to the site of injury. This observation supports the concept of a humoral mediator for capillary permeability and suggests that more pronounced changes can occur with larger burns. Nonetheless, extravasation of albumin appears to be short-lived.", "contents": "Relationship of burn size to vascular permeability changes in rats. Studies to determine the relationship between increased vascular permeability, water content and protein leakage, and burn size were performed on rats. Partial thickness scald burn covering 10, 20, 30 and 40 per cent of the rats' body surface area were induced in four groups of rats. A fifth group of rats served as the controls. Following the burn, all rats received intravenously fluid resuscitation. Burn edema was maximal at three hours postburn for the 30 and 40 per cent groups. The edema persisted throughout the 24 hour study period. No significant change in water content of unburned skin was demonstrated. Albumin leakage into injuried tissues was maximal at 30 minutes and disappeared by 12 hours postinjury. The size of the burn had no effect on 131Risa leakage into injuried tissues. Albumin leakage into noninjured tissues occurred only in the 30 and 40 per cent burn groups was mild compared with that in burned tissues and also disappeared by 12 hours postburn. Extravasation of albumin in lung, liver, spleen, kidney and muscle was mild and seen only in the 30 and 40 per cent burn groups. Except for a mild increase in water content of lung tissue in the 30 and 40 per cent groups only, no consistent increase in the water content of other organs was observed. While the size of the burn appears to have some influence on capillary permeability, burns up to 40 per cent of the body surface have only a mild and transient effect upon capillaries distant to the site of injury. This observation supports the concept of a humoral mediator for capillary permeability and suggests that more pronounced changes can occur with larger burns. Nonetheless, extravasation of albumin appears to be short-lived."}
{"id": "PMID:462351", "title": "Salivary gland drainage into the thyroglossal duct.", "content": "The clinical entity described consists of intralingual ectopic salivary glands and ducts draining into the thyroglossal duct. The value of preoperative sinograms to demonstrate the condition is important; the use of methylene blue at operation is advocated to ensure complete removal.", "contents": "Salivary gland drainage into the thyroglossal duct. The clinical entity described consists of intralingual ectopic salivary glands and ducts draining into the thyroglossal duct. The value of preoperative sinograms to demonstrate the condition is important; the use of methylene blue at operation is advocated to ensure complete removal."}
{"id": "PMID:462352", "title": "Prevention of radiation enteritis after abdominoperineal resection and radiotherapy.", "content": "The method described, aimed at preventing radiation enteritis in patients undergoing radiotherapy, postabdominoperineal resection is simple enough, does not materially prolong the time of operation and in our experience, thus far, seems to be effective.", "contents": "Prevention of radiation enteritis after abdominoperineal resection and radiotherapy. The method described, aimed at preventing radiation enteritis in patients undergoing radiotherapy, postabdominoperineal resection is simple enough, does not materially prolong the time of operation and in our experience, thus far, seems to be effective."}
{"id": "PMID:462353", "title": "Clinical aspects of grave pyogenic abscesses of the liver.", "content": "Solitary hepatic abscess has a favorable prognosis in contrast with multiple abscesses which generally are fatal. As compared with the classical cause of appendicitis, at present, abscesses are frequently related to biliary tract and diverticular disease. Occult or temporally remote processes are responsibile for many solitary abscesses. Lethality of multiple abscesses is related to fulminant hepatic and source sepsis, atypical syndromes, late diagnosis and difficult, complex treatment. Causative organisms are predominantly gram-negative and increasingly anaerobic, requiring special bacteriology for isolation. Various laboratory data are useful in diagnosis and prognosis, but liver scans and celiac angiography are critical procedures. Treatment aimed at lowering the mortality of multiple liver abscesses includes early diagnosis, surgical exploration and abscess drainage, direct bacterial identification emphasizing anaerobic techniques, intense specific antibiotic therapy and identification and definitive therapy of the seeding focus with special attention being given to the biliary tract.", "contents": "Clinical aspects of grave pyogenic abscesses of the liver. Solitary hepatic abscess has a favorable prognosis in contrast with multiple abscesses which generally are fatal. As compared with the classical cause of appendicitis, at present, abscesses are frequently related to biliary tract and diverticular disease. Occult or temporally remote processes are responsibile for many solitary abscesses. Lethality of multiple abscesses is related to fulminant hepatic and source sepsis, atypical syndromes, late diagnosis and difficult, complex treatment. Causative organisms are predominantly gram-negative and increasingly anaerobic, requiring special bacteriology for isolation. Various laboratory data are useful in diagnosis and prognosis, but liver scans and celiac angiography are critical procedures. Treatment aimed at lowering the mortality of multiple liver abscesses includes early diagnosis, surgical exploration and abscess drainage, direct bacterial identification emphasizing anaerobic techniques, intense specific antibiotic therapy and identification and definitive therapy of the seeding focus with special attention being given to the biliary tract."}
{"id": "PMID:462354", "title": "Changes in the coagulation of blood during resection of the abdominal aorta.", "content": "In 33 patients, a significant fall in the fibrinogen level occurred during an operation to replace the abdominal aorta by a bifurcated prosthesis. There was a similar, but less marked, fall in the fibrinogen level of ten patients having a femoropopliteal vein bypass. A concomitant drop in the plasma plasminogen value was also found. Results of specific laboratory tests for intravascular clotting and activation of fibrinolysis were negative. This suggests that fibrinogen may be removed by physical means. The fall in fibrinogen was so low in some patients that the routine administration of a conventional dosage of heparin could be dangerous.", "contents": "Changes in the coagulation of blood during resection of the abdominal aorta. In 33 patients, a significant fall in the fibrinogen level occurred during an operation to replace the abdominal aorta by a bifurcated prosthesis. There was a similar, but less marked, fall in the fibrinogen level of ten patients having a femoropopliteal vein bypass. A concomitant drop in the plasma plasminogen value was also found. Results of specific laboratory tests for intravascular clotting and activation of fibrinolysis were negative. This suggests that fibrinogen may be removed by physical means. The fall in fibrinogen was so low in some patients that the routine administration of a conventional dosage of heparin could be dangerous."}
{"id": "PMID:462355", "title": "Microbiology of superficial and deep tissues in infected diabetic gangrene.", "content": "In the current study, it is shown that, in the lesions of diabetic gangrene, there are usually mixed flora of two or more organisms. The organisms isolated from the presenting lesion may only partially reflect the organisms found in the deep tissues. Of the 58 cultures from the deep tissues, 11 of the isolates were anaerobes, but those anaerobes were never isolated as a pure culture. Bacterial synergism probably plays a role in infected diabetic gangrene. Maximizing the reduction of systemic infection and local cellulitis by elevation and appropriate antibiotic therapy results in improved wound healing in amputations of the lower extremities in patients with diabetic gangrene. However, apparent from this study, antibiotic therapy does not eradicate the organisms in the deep tissue, and surgical procedures are usually required for definitive treatment. Material from the surgically removed infected tissue should always be cultured for identification of existing organisms. When indicated, appropriate adjustment of antibiotic therapy, based upon these findings, should be prescribed.", "contents": "Microbiology of superficial and deep tissues in infected diabetic gangrene. In the current study, it is shown that, in the lesions of diabetic gangrene, there are usually mixed flora of two or more organisms. The organisms isolated from the presenting lesion may only partially reflect the organisms found in the deep tissues. Of the 58 cultures from the deep tissues, 11 of the isolates were anaerobes, but those anaerobes were never isolated as a pure culture. Bacterial synergism probably plays a role in infected diabetic gangrene. Maximizing the reduction of systemic infection and local cellulitis by elevation and appropriate antibiotic therapy results in improved wound healing in amputations of the lower extremities in patients with diabetic gangrene. However, apparent from this study, antibiotic therapy does not eradicate the organisms in the deep tissue, and surgical procedures are usually required for definitive treatment. Material from the surgically removed infected tissue should always be cultured for identification of existing organisms. When indicated, appropriate adjustment of antibiotic therapy, based upon these findings, should be prescribed."}
{"id": "PMID:462356", "title": "The use of radioactive oxygen to study muscle function in peripheral vascular disease.", "content": "We have use 15O to study the relationship between blood flow and oxygen utilization in the limbs of patients with peripheral vascular diseases. This technique is noninvasive and provides a useful method of studying the function of ischemic limbs.", "contents": "The use of radioactive oxygen to study muscle function in peripheral vascular disease. We have use 15O to study the relationship between blood flow and oxygen utilization in the limbs of patients with peripheral vascular diseases. This technique is noninvasive and provides a useful method of studying the function of ischemic limbs."}
{"id": "PMID:462357", "title": "Primary adenocarcinoma of the vagina.", "content": "The characteristics and treatment of eight patients with primary adenocarcinoma of the vagina were reviewed. Cytologic smears of the vagina aided in the diagnosis of adenocarcinoma but, less often, detected vaginal adenosis. Good correlation existed between the degree of differentiation of the primary tumor and the tendency toward lymphatic spread. The clinical stage, size of the primary lesion, presence and duration of symptoms and lymphangiographic findings were not helpful in this regard. Pretreatment lymphadenectomy as a basis for operative staging demonstrated the critical importance of the status of the lymph nodes in the planning of therapy and determination of prognosis. Vaginal reconstruction and ovarian preservation facilitated rehabilitation.", "contents": "Primary adenocarcinoma of the vagina. The characteristics and treatment of eight patients with primary adenocarcinoma of the vagina were reviewed. Cytologic smears of the vagina aided in the diagnosis of adenocarcinoma but, less often, detected vaginal adenosis. Good correlation existed between the degree of differentiation of the primary tumor and the tendency toward lymphatic spread. The clinical stage, size of the primary lesion, presence and duration of symptoms and lymphangiographic findings were not helpful in this regard. Pretreatment lymphadenectomy as a basis for operative staging demonstrated the critical importance of the status of the lymph nodes in the planning of therapy and determination of prognosis. Vaginal reconstruction and ovarian preservation facilitated rehabilitation."}
{"id": "PMID:462358", "title": "Comparative results of three different techniques for colonic anastomosis in the dog.", "content": "Three different techniques of colonic anastomosis were evaluated in the dog. Left hemicolectomy and two layer anastomosis were performed in ten dogs, one layer anastomosis in ten dogs and sleeve anastomosis in ten dogs. In each group, five dogs were sacrificed on the second postoperative day, and the anastomotic specimen was studied by a bursting test. The remaining five dogs were sacrificed seven days after operation, and the specimen was studied by hydroxyproline dosage and by microscopy. The bursting test showed a significant difference between sleeve anastamosis and other techniques. The results of hydroxyproline dosage showed no significant differences between the different anastamotic techniques.", "contents": "Comparative results of three different techniques for colonic anastomosis in the dog. Three different techniques of colonic anastomosis were evaluated in the dog. Left hemicolectomy and two layer anastomosis were performed in ten dogs, one layer anastomosis in ten dogs and sleeve anastomosis in ten dogs. In each group, five dogs were sacrificed on the second postoperative day, and the anastomotic specimen was studied by a bursting test. The remaining five dogs were sacrificed seven days after operation, and the specimen was studied by hydroxyproline dosage and by microscopy. The bursting test showed a significant difference between sleeve anastamosis and other techniques. The results of hydroxyproline dosage showed no significant differences between the different anastamotic techniques."}
{"id": "PMID:462361", "title": "Microsurgical removal of lumbar herniated nucleus pulposus.", "content": "An operating microscope and special instruments make it possible to remove a herniated nucleus pulposus without laminectomy through a 1 inch skin incision. In a follow-up study of 200 patients operated upon over a two and three-fourths year period, the cure rate has been 96 per cent and the postoperative stay reduced to less than three days. One year after operation, all noncompensation patients were working as were 80 per cent of the compensation patients.", "contents": "Microsurgical removal of lumbar herniated nucleus pulposus. An operating microscope and special instruments make it possible to remove a herniated nucleus pulposus without laminectomy through a 1 inch skin incision. In a follow-up study of 200 patients operated upon over a two and three-fourths year period, the cure rate has been 96 per cent and the postoperative stay reduced to less than three days. One year after operation, all noncompensation patients were working as were 80 per cent of the compensation patients."}
{"id": "PMID:462362", "title": "A new vascular access procedure for immediate as well as chronic hemodialysis.", "content": "A new vascular access procedure consists of attaching an arteriovenous shunt to a subcutaneous arteriovenous fistula. The procedure was designed for patients who need immediate as well as long term hemodialysis and in whom the Brescia-Cimino type of fistula could not be done. Secondary objectives for this new procedure were a prolonged patency interval and a lower complication rate of arteriovenous fistulas. Six patients underwent this procedure and were observed from two to five months. Five shunts remained patent for a minimum of two weeks. Five patients have presently functioning fistulas, and in three of these, both shunts and fistulas remain patent. Two of the presently functioning shunts required declotting. Two shunts were removed when neither declotting nor revision was possible, and one shunt was removed because of infection. One fistula was lost due to a technical error, and two of the five presently functioning fistulas have required declotting. We believe clotting was due chiefly to the fact that patients selected for this procedure were from the group who had multiple previous clotting episodes and vascular access procedures. It remains to be seen whether or not more careful patient selection will accomplish the secondary objectives.", "contents": "A new vascular access procedure for immediate as well as chronic hemodialysis. A new vascular access procedure consists of attaching an arteriovenous shunt to a subcutaneous arteriovenous fistula. The procedure was designed for patients who need immediate as well as long term hemodialysis and in whom the Brescia-Cimino type of fistula could not be done. Secondary objectives for this new procedure were a prolonged patency interval and a lower complication rate of arteriovenous fistulas. Six patients underwent this procedure and were observed from two to five months. Five shunts remained patent for a minimum of two weeks. Five patients have presently functioning fistulas, and in three of these, both shunts and fistulas remain patent. Two of the presently functioning shunts required declotting. Two shunts were removed when neither declotting nor revision was possible, and one shunt was removed because of infection. One fistula was lost due to a technical error, and two of the five presently functioning fistulas have required declotting. We believe clotting was due chiefly to the fact that patients selected for this procedure were from the group who had multiple previous clotting episodes and vascular access procedures. It remains to be seen whether or not more careful patient selection will accomplish the secondary objectives."}
{"id": "PMID:462363", "title": "Choroidal occlusive disease in sickle cell hemoglobinopathies.", "content": "Two distinct episodes of posterior ciliary artery occlusion were studied in a 32-year-old man with hemoglobin SS disease and multiple episodes of amaurosis fugax. Although posterior ciliary artery occlusions have been observed following photocoagulation of sickle cell retinopathy, their spontaneous evolution in patients with sickling hemoglobinopathies has received little attention. The manifestations of posterior ciliar artery occlusion seen in this case and in other clinical and experimental situations are reviewed. Histopathologic examination of three additional eyes of patients with sickle hemoglobinopathies revealed changes which may have been the result of previous small posterior ciliary artery occlusions or small vessel occlusive disease related to the sickling hemoglobinopathies; these cases are also reported.", "contents": "Choroidal occlusive disease in sickle cell hemoglobinopathies. Two distinct episodes of posterior ciliary artery occlusion were studied in a 32-year-old man with hemoglobin SS disease and multiple episodes of amaurosis fugax. Although posterior ciliary artery occlusions have been observed following photocoagulation of sickle cell retinopathy, their spontaneous evolution in patients with sickling hemoglobinopathies has received little attention. The manifestations of posterior ciliar artery occlusion seen in this case and in other clinical and experimental situations are reviewed. Histopathologic examination of three additional eyes of patients with sickle hemoglobinopathies revealed changes which may have been the result of previous small posterior ciliary artery occlusions or small vessel occlusive disease related to the sickling hemoglobinopathies; these cases are also reported."}
{"id": "PMID:462364", "title": "Photographic investigation of the human lens and cataract.", "content": "Slit-image photography of the anterior segment of the eye can provide important information about the dimensions and optical densities of the refractive structures. It is crucial that the slit-image photograph be in perfect focus and that the slit-beam cut in exactly the same section (along the optic axis) on each occasion. The equipment, principles and techniques which make such precision slit-imagephotography possible  are discussed. Clinical and research applications, especially regarding the human lens and cataract, are summarized.", "contents": "Photographic investigation of the human lens and cataract. Slit-image photography of the anterior segment of the eye can provide important information about the dimensions and optical densities of the refractive structures. It is crucial that the slit-image photograph be in perfect focus and that the slit-beam cut in exactly the same section (along the optic axis) on each occasion. The equipment, principles and techniques which make such precision slit-imagephotography possible  are discussed. Clinical and research applications, especially regarding the human lens and cataract, are summarized."}
{"id": "PMID:462365", "title": "Pseudotumor cerebri.", "content": "The authors evaluate the case of a 20-year-old, four-month-pregnant woman who presented with papilledema and visual field loss, and developed headache, intermittent diplopia and impairment of color vision over the next month. The procedures and considerations leading to the diagnosis of pseudotumor cerebri are discussed, as are the various modes of therapy and their indications.", "contents": "Pseudotumor cerebri. The authors evaluate the case of a 20-year-old, four-month-pregnant woman who presented with papilledema and visual field loss, and developed headache, intermittent diplopia and impairment of color vision over the next month. The procedures and considerations leading to the diagnosis of pseudotumor cerebri are discussed, as are the various modes of therapy and their indications."}
{"id": "PMID:462371", "title": "The nonportal origin of the factors initiating hepatic regeneration.", "content": "To determine the site of origin of the factors that initiate deoxyribonucleic acid (DNA) synthesis in the liver after partial hepatectomy, normal rats were cross-circulated with totally hepatectomized rats. Half of the hepatectomized rats had also undergone excision of all of the portal organs. After 48 hours of cross-circulation, active DNA synthesis and other evidences of hepatic regeneration were found in normal rats cross-circulated with the hepatectomized portally eviscerated rats. This demonstrates that a blood-borne factor that does not arise from the portal organs is capable of initiating hepatic regeneration. When a normal rat was cross-circulated with a hepatectomized rat with the portal organs still present, hepatic regeneration occurred but was significantly less than when the portal organs had been removed. It is postulated that under these experimental conditions portal factors from the normal rat have a permissive role that allows active regeneration when initiating factors are furnished from the hepatectomized rat. Additional portal organs in the hepatectomized rat decreased DNA synthesis, possibly by alterations of the insulin/glucagon ratio.", "contents": "The nonportal origin of the factors initiating hepatic regeneration. To determine the site of origin of the factors that initiate deoxyribonucleic acid (DNA) synthesis in the liver after partial hepatectomy, normal rats were cross-circulated with totally hepatectomized rats. Half of the hepatectomized rats had also undergone excision of all of the portal organs. After 48 hours of cross-circulation, active DNA synthesis and other evidences of hepatic regeneration were found in normal rats cross-circulated with the hepatectomized portally eviscerated rats. This demonstrates that a blood-borne factor that does not arise from the portal organs is capable of initiating hepatic regeneration. When a normal rat was cross-circulated with a hepatectomized rat with the portal organs still present, hepatic regeneration occurred but was significantly less than when the portal organs had been removed. It is postulated that under these experimental conditions portal factors from the normal rat have a permissive role that allows active regeneration when initiating factors are furnished from the hepatectomized rat. Additional portal organs in the hepatectomized rat decreased DNA synthesis, possibly by alterations of the insulin/glucagon ratio."}
{"id": "PMID:462372", "title": "Serum ribonuclease elevations and pancreatic necrosis in acute pancreatitis.", "content": "Pancreatic necrosis is a principal determinant of the severity, duration, and infectious complications of acute pancreatitis. There has been no objective index for pancreatic necrosis, and its recognition has necessarily rested upon nonspecific clinical signs, including later deterioration or appearance of sepsis. In search of such an index, we have measured serum levels of a poly-[C]-specific acid ribonuclease (RNase) in 38 patients with acute pancreatitis, 12 patients with chronic pancreatitis, and 50 control patients. The values in chronic pancreatitis (mean, 52 units; range, 33 to 80 units) were within observed normal limits (mean, 51; range, 17 to 94). The values in acute pancreatitis segregated into two groups, normal values (group A) and high values (group B). Of 25 patients in group A (mean, 46; range, 19 to 87), only one developed evidence of pancreatic necrosis or abscess. In contrast, of the 13 patients in group B (mean, 192, range, 98 to 385), 11 required surgical debridement/drainage for pancreatic necrosis (six) or abscess (five) (P less than 0.001). Each of the other two patients had prolonged pancreatic inflammation with fever and a pancreatic mass which persisted for more than 2 weeks. RNase levels in group B patients rose within a few days after onset of pancreatitis and tended to parallel the clinical course. These findings suggest that measurement of serum RNase in acute pancreatitis gives a reliable indication of pancreatic necrosis. Therefore RNase determinations should be of value for earlier identification and monitoring of patients at high risk of late complications, and for helping to select those who will benefit from early debridement before secondary infection occurs.", "contents": "Serum ribonuclease elevations and pancreatic necrosis in acute pancreatitis. Pancreatic necrosis is a principal determinant of the severity, duration, and infectious complications of acute pancreatitis. There has been no objective index for pancreatic necrosis, and its recognition has necessarily rested upon nonspecific clinical signs, including later deterioration or appearance of sepsis. In search of such an index, we have measured serum levels of a poly-[C]-specific acid ribonuclease (RNase) in 38 patients with acute pancreatitis, 12 patients with chronic pancreatitis, and 50 control patients. The values in chronic pancreatitis (mean, 52 units; range, 33 to 80 units) were within observed normal limits (mean, 51; range, 17 to 94). The values in acute pancreatitis segregated into two groups, normal values (group A) and high values (group B). Of 25 patients in group A (mean, 46; range, 19 to 87), only one developed evidence of pancreatic necrosis or abscess. In contrast, of the 13 patients in group B (mean, 192, range, 98 to 385), 11 required surgical debridement/drainage for pancreatic necrosis (six) or abscess (five) (P less than 0.001). Each of the other two patients had prolonged pancreatic inflammation with fever and a pancreatic mass which persisted for more than 2 weeks. RNase levels in group B patients rose within a few days after onset of pancreatitis and tended to parallel the clinical course. These findings suggest that measurement of serum RNase in acute pancreatitis gives a reliable indication of pancreatic necrosis. Therefore RNase determinations should be of value for earlier identification and monitoring of patients at high risk of late complications, and for helping to select those who will benefit from early debridement before secondary infection occurs."}
{"id": "PMID:462366", "title": "Longterm timolol therapy.", "content": "Of 145 patients registered for an investigation of timolol, 27 patients were dropped for unrelated reasons. Among the remaining 118 patients (212 eyes), a significant reduction in intraocular pressure resulted from twice-a-day topical instillation of 0.25 or 0.5% timolol to ocular hypertensives, primary open-angle glaucoma or aphakic glaucoma patients. Outflow facility improved only slightly, but remained improved throughout the course of this study. A significant reduction in aqueous secretion, however, was the predominant cause of intraocular pressure reduction. Timolol alone controlled intraocular pressure in 87% of ocular hypertensives, in 68% of patients with chronic open-angle glaucoma and in 68% of patients with angle-unobstructed aphakic glaucomas. The addition of epinephrine brought the intraocular pressure to less than 22 mm Hg in 85% of the total group of patients.", "contents": "Longterm timolol therapy. Of 145 patients registered for an investigation of timolol, 27 patients were dropped for unrelated reasons. Among the remaining 118 patients (212 eyes), a significant reduction in intraocular pressure resulted from twice-a-day topical instillation of 0.25 or 0.5% timolol to ocular hypertensives, primary open-angle glaucoma or aphakic glaucoma patients. Outflow facility improved only slightly, but remained improved throughout the course of this study. A significant reduction in aqueous secretion, however, was the predominant cause of intraocular pressure reduction. Timolol alone controlled intraocular pressure in 87% of ocular hypertensives, in 68% of patients with chronic open-angle glaucoma and in 68% of patients with angle-unobstructed aphakic glaucomas. The addition of epinephrine brought the intraocular pressure to less than 22 mm Hg in 85% of the total group of patients."}
{"id": "PMID:462367", "title": "Timolol plus maximum tolerated antiglaucoma therapy: a one-year followup study.", "content": "Thirty-three eyes of 22 patients with primary open-angle glaucoma or severe ocular hypertension had topical timolol maleate added to their maximally tolerated medical regimens. With minimal side effects, 25 eyes (76%) could be treated medically for one year, and only eight eyes (24%) required filtering surgery. Thirteen (39%) of the medically treated eyes achieved intraocular pressures of 21 mm Hg or less. After the addition of timolol, 17 of the 33 eyes (52%) completed the one-year protocol with final mean intraocular pressure decreases of 5 mm Hg or more on medical therapy.", "contents": "Timolol plus maximum tolerated antiglaucoma therapy: a one-year followup study. Thirty-three eyes of 22 patients with primary open-angle glaucoma or severe ocular hypertension had topical timolol maleate added to their maximally tolerated medical regimens. With minimal side effects, 25 eyes (76%) could be treated medically for one year, and only eight eyes (24%) required filtering surgery. Thirteen (39%) of the medically treated eyes achieved intraocular pressures of 21 mm Hg or less. After the addition of timolol, 17 of the 33 eyes (52%) completed the one-year protocol with final mean intraocular pressure decreases of 5 mm Hg or more on medical therapy."}
{"id": "PMID:462373", "title": "Negative inotropic effect of albumin resuscitation for shock.", "content": "The inotropic effects of albumin were studied in 94 seriously injured patients who received an average of 14.5 transfusions, 9.2 liters of crystalloid and 0.9 liters of plasma prior to end of operation; 46 patients, by random selection, received added albumin averaging 31 gm during operation, 198 gm during the early postoperative period of extravascular fluid sequestration, and 395 gm during the first 4 days of the later fluid mobilization period. Left ventricular stroke work index (LVSWI) was plotted against pulmonary wedge pressure (Ppw) in 22 patients who had indwelling thermistor pulmonary artery catheters at the time of the first study. Calculated heart work units (WU) were derived from the pulse pressure, mean arterial pressure, pulse rate, and central venous pressure (CVP) in patients without LVSWI measurements. Albumin supplementation increased serum albumin (4.2 vs. 2.9 gm%), plasma volume, CVP (15 vs. 9 cm H2O), but did not alter red cell volume (1,531 vs. 1,519 ml). The ratio of LVSWI/Ppw fell in albumin patients (1.9 +/- 1.6 vs. 4.8 +/- 1.8), and the ratio of WU/CVP was significantly depressed in albumin patients (4.9 +/- 2.3 vs. 7.3 +/- 2.1). The slopes of the LVSWI/Ppw and WU/CVP were shifted to the right in albumin patients. This negative inotropic effect was associated with impaired oxygenation, as reflected by an increased ratio of inspired oxygen per arterial oxygen tension (0.62 +/- 0.06 vs. 0.33 +/- 0.1). Finally, 24 of the 46 albumin-treated patients were digitalized for heart failure, compared to only 11 of the 48 nonalbumin patients. Pending subsequent studies, albumin should be considered a potentially negative inotropic agent.", "contents": "Negative inotropic effect of albumin resuscitation for shock. The inotropic effects of albumin were studied in 94 seriously injured patients who received an average of 14.5 transfusions, 9.2 liters of crystalloid and 0.9 liters of plasma prior to end of operation; 46 patients, by random selection, received added albumin averaging 31 gm during operation, 198 gm during the early postoperative period of extravascular fluid sequestration, and 395 gm during the first 4 days of the later fluid mobilization period. Left ventricular stroke work index (LVSWI) was plotted against pulmonary wedge pressure (Ppw) in 22 patients who had indwelling thermistor pulmonary artery catheters at the time of the first study. Calculated heart work units (WU) were derived from the pulse pressure, mean arterial pressure, pulse rate, and central venous pressure (CVP) in patients without LVSWI measurements. Albumin supplementation increased serum albumin (4.2 vs. 2.9 gm%), plasma volume, CVP (15 vs. 9 cm H2O), but did not alter red cell volume (1,531 vs. 1,519 ml). The ratio of LVSWI/Ppw fell in albumin patients (1.9 +/- 1.6 vs. 4.8 +/- 1.8), and the ratio of WU/CVP was significantly depressed in albumin patients (4.9 +/- 2.3 vs. 7.3 +/- 2.1). The slopes of the LVSWI/Ppw and WU/CVP were shifted to the right in albumin patients. This negative inotropic effect was associated with impaired oxygenation, as reflected by an increased ratio of inspired oxygen per arterial oxygen tension (0.62 +/- 0.06 vs. 0.33 +/- 0.1). Finally, 24 of the 46 albumin-treated patients were digitalized for heart failure, compared to only 11 of the 48 nonalbumin patients. Pending subsequent studies, albumin should be considered a potentially negative inotropic agent."}
{"id": "PMID:462369", "title": "The role of clinical drug trial methodology with respect to studies of new drugs. Clinical trials of timolol.", "content": "The use of the Randomized Clinical Trial (RCT) to assess the safety and efficacy of a new drug or treatment can provide a clear evaluation of the relative benefits and risks of the new therapy as compared to the longer-established treatment methodology. In some cases, such as insulin, the benefits are immediately apparent; in other cases, such as photocoagulation for proliferative diabetic retinopathy, the consensus is not so easily obtained, and the clinical trial can supply the answer. Furthermore, the RCT provides the opportunity to identify adverse reactions, in particular those which present with a seemingly unrelated or unexpected set of symptoms. In conducting clinical trials of timolol, researchers must be alert for beta-adrenergic antagonist effects, such as were seen with practolol, and should be especially careful to note early, mild reactions which may be the forerunners of later, more severe or irreversible adverse effects.", "contents": "The role of clinical drug trial methodology with respect to studies of new drugs. Clinical trials of timolol. The use of the Randomized Clinical Trial (RCT) to assess the safety and efficacy of a new drug or treatment can provide a clear evaluation of the relative benefits and risks of the new therapy as compared to the longer-established treatment methodology. In some cases, such as insulin, the benefits are immediately apparent; in other cases, such as photocoagulation for proliferative diabetic retinopathy, the consensus is not so easily obtained, and the clinical trial can supply the answer. Furthermore, the RCT provides the opportunity to identify adverse reactions, in particular those which present with a seemingly unrelated or unexpected set of symptoms. In conducting clinical trials of timolol, researchers must be alert for beta-adrenergic antagonist effects, such as were seen with practolol, and should be especially careful to note early, mild reactions which may be the forerunners of later, more severe or irreversible adverse effects."}
{"id": "PMID:462368", "title": "Effects of iris pigmentation on response of ocular pressure to timolol.", "content": "Timolol ophthalmic solution effectively reduced mean intraocular pressure (IOP) in Negro and Caucasian patients with chronic open angle glaucoma and untreated IOP of 22 mm Hg or higher. No differences in pressure lowering response were observed among the races although the Negroes required somewhat higher timolol concentrations to produce similar reductions from baseline IOP. Negro patients also appeared to have a more severe disease as indicated by the number of prior antiglaucoma drugs required and the untreated (baseline) pressures. An unexpected finding was the significantly greater number of Caucasians with dark irides who were discontinued from therapy because of inadequate pressure reduction.", "contents": "Effects of iris pigmentation on response of ocular pressure to timolol. Timolol ophthalmic solution effectively reduced mean intraocular pressure (IOP) in Negro and Caucasian patients with chronic open angle glaucoma and untreated IOP of 22 mm Hg or higher. No differences in pressure lowering response were observed among the races although the Negroes required somewhat higher timolol concentrations to produce similar reductions from baseline IOP. Negro patients also appeared to have a more severe disease as indicated by the number of prior antiglaucoma drugs required and the untreated (baseline) pressures. An unexpected finding was the significantly greater number of Caucasians with dark irides who were discontinued from therapy because of inadequate pressure reduction."}
{"id": "PMID:462374", "title": "The leukocyte migration inhibition response to certain breast cancer-related antigens (MCF-7 and MuMTV): their potential as discriminants.", "content": "Certain oncogenic viruses have been implicated in human breast cancer, including the murine mammary tumor virus (MuMTV) and the Mason-Pfizer monkey virus (MPMV). We have used the leukocyte migration inhibition (LMI) response to assay the response to several potential breast cancer-related antigens, including MuMTV, MPMV, and a breast cancer cultured cell line, MCF-7, in 96 breast cancer patients, in 32 women with benign breast disease, and in 67 normal women. The lowest tenth percentile of control (LMI) responses was used as the cutoff point to designate responders. Breast cancer patients showed significant responses to MuMTV (49% and to MCF-7 (50%), but not to MPMV (29%). In a paired-antigen study using MuMTV and MCF-7, 75% of the breast cancer patients responded, versus 18% of the normal women (P less than 0.0050). The potential for this assay to distinguish \"normal\" from \"breast cancer\" was analyzed using a migration index derived from discriminant analysis. The ability of the assay to discriminate \"normal\" from \"cancer\" was significant (P less than 0.001) and showed a sensitivity of detecting \"cancer\" of 75%. The overall responses to MuMTV and MCF-7 were analyzed with reference to certain prognostic factors, but showed no relation to age, menstrual status, estrogen receptor status, or stage of disease. The above reactions suggest that a large proportion of breast cancer patients exhibit presensitization to antigenfs found in MuMTV and MCF-7, which may be cross-reactive with antigens in the primary cancer. These responses appear to be independent of major prognostic variables. Further refinement of this assay may yield one which is more highly discriminating for breast cancer.", "contents": "The leukocyte migration inhibition response to certain breast cancer-related antigens (MCF-7 and MuMTV): their potential as discriminants. Certain oncogenic viruses have been implicated in human breast cancer, including the murine mammary tumor virus (MuMTV) and the Mason-Pfizer monkey virus (MPMV). We have used the leukocyte migration inhibition (LMI) response to assay the response to several potential breast cancer-related antigens, including MuMTV, MPMV, and a breast cancer cultured cell line, MCF-7, in 96 breast cancer patients, in 32 women with benign breast disease, and in 67 normal women. The lowest tenth percentile of control (LMI) responses was used as the cutoff point to designate responders. Breast cancer patients showed significant responses to MuMTV (49% and to MCF-7 (50%), but not to MPMV (29%). In a paired-antigen study using MuMTV and MCF-7, 75% of the breast cancer patients responded, versus 18% of the normal women (P less than 0.0050). The potential for this assay to distinguish \"normal\" from \"breast cancer\" was analyzed using a migration index derived from discriminant analysis. The ability of the assay to discriminate \"normal\" from \"cancer\" was significant (P less than 0.001) and showed a sensitivity of detecting \"cancer\" of 75%. The overall responses to MuMTV and MCF-7 were analyzed with reference to certain prognostic factors, but showed no relation to age, menstrual status, estrogen receptor status, or stage of disease. The above reactions suggest that a large proportion of breast cancer patients exhibit presensitization to antigenfs found in MuMTV and MCF-7, which may be cross-reactive with antigens in the primary cancer. These responses appear to be independent of major prognostic variables. Further refinement of this assay may yield one which is more highly discriminating for breast cancer."}
{"id": "PMID:462370", "title": "Patient compliance and the \"usefulness product\" of timolol.", "content": "The practical value of any therapy depends upon a combination of effectiveness of the agent (or procedure) and the extent to which the patient complies in its proper administration. Thus, it might be said that the multiple of effectiveness times compliance equals the \"usefulness product.\" Among the various glaucoma therapies, this product seems to range from about 30%--90%. Timolol's effectiveness in lowering intraocular pressure combined with its low incidence of side effects yields a high usefulness product, and this may decrease the incidence of surgery required for the treatment of the open-angle glaucomas.", "contents": "Patient compliance and the \"usefulness product\" of timolol. The practical value of any therapy depends upon a combination of effectiveness of the agent (or procedure) and the extent to which the patient complies in its proper administration. Thus, it might be said that the multiple of effectiveness times compliance equals the \"usefulness product.\" Among the various glaucoma therapies, this product seems to range from about 30%--90%. Timolol's effectiveness in lowering intraocular pressure combined with its low incidence of side effects yields a high usefulness product, and this may decrease the incidence of surgery required for the treatment of the open-angle glaucomas."}
{"id": "PMID:462375", "title": "The effects of early pneumonectomy on the remaining pulmonary parenchyma.", "content": "To determine the age-related response of the remaining pulmonary parenchyma to lung resection, a matched set of 24 purebred beagle dogs underwent pneumonectomy at 6 to 10 weeks of age (group I) or at 1 year of age (group II). Eight unoperated adult beagles served as controls (group III). One year after pneumonectomy, pulmonary hemodynamics in group I were the same as those in older animals shortly after pneumonectomy and in normal control animals. Functional residual capacity, total lung capacity, single breath diffusing capacity for carbon monoxide, and static lung compliance were measured in all three groups while anesthetized and intubated. Lung volumes and diffusion capacity for carbon monoxide in group I did not differ significantly from those of control animals. Group I animals did have a lower lung compliance than the control group, but significantly greater than the group II adult pneumonectomy dogs. The adult pneumonectomy dogs (group II) had significantly lower values for lung volumes, diffusion capacity, and compliance when compared to the control group. Arterial blood gases were not significantly different among the groups. It is concluded that beagles undergoing early pneumonectomy have an increase of alveoli beyond the normal complement in the remaining lung with concomitant remodeling of the pulmonary capillary bed resulting in normal lung volumes, diffusing capacity, and gas exchange.", "contents": "The effects of early pneumonectomy on the remaining pulmonary parenchyma. To determine the age-related response of the remaining pulmonary parenchyma to lung resection, a matched set of 24 purebred beagle dogs underwent pneumonectomy at 6 to 10 weeks of age (group I) or at 1 year of age (group II). Eight unoperated adult beagles served as controls (group III). One year after pneumonectomy, pulmonary hemodynamics in group I were the same as those in older animals shortly after pneumonectomy and in normal control animals. Functional residual capacity, total lung capacity, single breath diffusing capacity for carbon monoxide, and static lung compliance were measured in all three groups while anesthetized and intubated. Lung volumes and diffusion capacity for carbon monoxide in group I did not differ significantly from those of control animals. Group I animals did have a lower lung compliance than the control group, but significantly greater than the group II adult pneumonectomy dogs. The adult pneumonectomy dogs (group II) had significantly lower values for lung volumes, diffusion capacity, and compliance when compared to the control group. Arterial blood gases were not significantly different among the groups. It is concluded that beagles undergoing early pneumonectomy have an increase of alveoli beyond the normal complement in the remaining lung with concomitant remodeling of the pulmonary capillary bed resulting in normal lung volumes, diffusing capacity, and gas exchange."}
{"id": "PMID:462376", "title": "Inhibition of basal and meal-stimulated choleresis by somatostatin.", "content": "The effect of somatostatin, an inhibitor of release of a number of gastrointestinal and other hormones, on choleresis was investigated in chronic, bile fistula dogs with taurocholate-stabilized bile flow. Somatostatin inhibited both fasting and meal-stimulated choleresis, and bile flows during somatostatin inhibition of both fasting and fed dogs were similar, suggesting a complete suppression of factors causing feeding choleresis. Although a transient decrease in bile salt output was observed, bile salt output was unaffected during most of the period of bile flow inhibition. Hormone suppression by somatostatin, indicated by measurement of serum insulin, occurred over a similar time course as inhibition of choleresis. These observations provide further evidence for physiological humoral regulation of choleresis.", "contents": "Inhibition of basal and meal-stimulated choleresis by somatostatin. The effect of somatostatin, an inhibitor of release of a number of gastrointestinal and other hormones, on choleresis was investigated in chronic, bile fistula dogs with taurocholate-stabilized bile flow. Somatostatin inhibited both fasting and meal-stimulated choleresis, and bile flows during somatostatin inhibition of both fasting and fed dogs were similar, suggesting a complete suppression of factors causing feeding choleresis. Although a transient decrease in bile salt output was observed, bile salt output was unaffected during most of the period of bile flow inhibition. Hormone suppression by somatostatin, indicated by measurement of serum insulin, occurred over a similar time course as inhibition of choleresis. These observations provide further evidence for physiological humoral regulation of choleresis."}
{"id": "PMID:462377", "title": "The effect of plasma oncotic pressure on the pulmonary microcirculation after hemorrhagic shock.", "content": "Plasma oncotic pressure is considered to be an important factor in controlling lung water after hemorrhagic shock. However, it is the gradient between plasma and interstitial oncotic pressure which affects the pulmonary transvascular fluid filtration rate, Qf. Our objective was to determine the effect of decreasing plasma oncotic pressure, pip, on Qf, on interstitial oncotic pressure pii, and on the oncotic gradient. Chronic lung lymph fistulas were created in 16 sheep. Lymph flow, a reliable index of Qf, plasma and lymph (equal to interstitial) oncotic pressures, and vascular pressures were monitored in unanesthetized sheep, before and during hemorrhagic shock (50% blood volume), during resuscitation (3 hours), and during recovery (24 hours). Resuscitation was either with shed blood or lactated Ringer's solution in sufficient quantity to return left atrial pressure and cardiac output to baseline levels. During resuscitation with blood, lymph flow increased by 115%. The pip remained constant, while pii decreased, increasing the oncotic gradient. Crystalloid resuscitation produced on increase in lymph flow equal to that in the blood group at 120% over baseline; however, pip decreased by 50%, producing an oncotic gradient 4 mm Hg less than that of blood group. This was not reflected by a difference in Qf between the groups. During recovery Qf returned to baseline in the blood group and in most of the crystalloid group, as the oncotic gradient returned to baseline, despite a significant decrease in pip due to a compensatory decrease in pii. We conclude that during resuscitation Qf does not appear to be increased by a decrease in the oncotic gradient. During recovery a major decrease in pip can be compensated for rapidly by a decrease in pii, leading to no change in interstitial fluid content.", "contents": "The effect of plasma oncotic pressure on the pulmonary microcirculation after hemorrhagic shock. Plasma oncotic pressure is considered to be an important factor in controlling lung water after hemorrhagic shock. However, it is the gradient between plasma and interstitial oncotic pressure which affects the pulmonary transvascular fluid filtration rate, Qf. Our objective was to determine the effect of decreasing plasma oncotic pressure, pip, on Qf, on interstitial oncotic pressure pii, and on the oncotic gradient. Chronic lung lymph fistulas were created in 16 sheep. Lymph flow, a reliable index of Qf, plasma and lymph (equal to interstitial) oncotic pressures, and vascular pressures were monitored in unanesthetized sheep, before and during hemorrhagic shock (50% blood volume), during resuscitation (3 hours), and during recovery (24 hours). Resuscitation was either with shed blood or lactated Ringer's solution in sufficient quantity to return left atrial pressure and cardiac output to baseline levels. During resuscitation with blood, lymph flow increased by 115%. The pip remained constant, while pii decreased, increasing the oncotic gradient. Crystalloid resuscitation produced on increase in lymph flow equal to that in the blood group at 120% over baseline; however, pip decreased by 50%, producing an oncotic gradient 4 mm Hg less than that of blood group. This was not reflected by a difference in Qf between the groups. During recovery Qf returned to baseline in the blood group and in most of the crystalloid group, as the oncotic gradient returned to baseline, despite a significant decrease in pip due to a compensatory decrease in pii. We conclude that during resuscitation Qf does not appear to be increased by a decrease in the oncotic gradient. During recovery a major decrease in pip can be compensated for rapidly by a decrease in pii, leading to no change in interstitial fluid content."}
{"id": "PMID:462378", "title": "Effect of vagal stimulation on pancreatic secretion and on blood levels of gastrin, cholecystokinin, secretin, vasoactive intestinal peptide, and somatostatin.", "content": "We investigated in dogs the effect of graded frequencies of electrical vagal stimulation (1.5, 3, 6, and 12 cps) on pancreatic exocrine secretion and on portal blood levels of gastrin, secretin, cholecystokinin (CCK), vasoactive intestinal peptide (VIP), and somatostatin (STS). Stimuli of all four frequencies, each with a duration of 5 minutes, were applied with a secretin background of 0.25 CU/kg-hr, and one stimulatory period of 12 cps was applied without a secretin background. With secretin, a significant, frequency-dependent increase of volume and of pancreatic protein secretion occurred from 3 to 12 cps. Gastrin values increased significantly at all frequencies. VIP and STS increased significantly with 3, 6, and 12 cps. Maximal responses for gastrin, VIP, and STS were observed with 6 cps. Peak values for gastrin and VIP were found during stimulation, whereas STS peaked after the end of the stimulatory period. The integrated responses of gastrin and STS showed significant correlation (P less than 0.01). The results suggest that vagally induced pancreatic response is only partially mediated by gastrin and perhaps VIP, and that endogenous gastrin may be one of the releasing factors for somatostatin. Plasma levels of CCK and secretin did not change after electrical stimulation, which provides direct evidence that their release is unlikely to be under vagal control, and that CCK does not mediate the protein secretion obtained after electrical stimulation of the vagus.", "contents": "Effect of vagal stimulation on pancreatic secretion and on blood levels of gastrin, cholecystokinin, secretin, vasoactive intestinal peptide, and somatostatin. We investigated in dogs the effect of graded frequencies of electrical vagal stimulation (1.5, 3, 6, and 12 cps) on pancreatic exocrine secretion and on portal blood levels of gastrin, secretin, cholecystokinin (CCK), vasoactive intestinal peptide (VIP), and somatostatin (STS). Stimuli of all four frequencies, each with a duration of 5 minutes, were applied with a secretin background of 0.25 CU/kg-hr, and one stimulatory period of 12 cps was applied without a secretin background. With secretin, a significant, frequency-dependent increase of volume and of pancreatic protein secretion occurred from 3 to 12 cps. Gastrin values increased significantly at all frequencies. VIP and STS increased significantly with 3, 6, and 12 cps. Maximal responses for gastrin, VIP, and STS were observed with 6 cps. Peak values for gastrin and VIP were found during stimulation, whereas STS peaked after the end of the stimulatory period. The integrated responses of gastrin and STS showed significant correlation (P less than 0.01). The results suggest that vagally induced pancreatic response is only partially mediated by gastrin and perhaps VIP, and that endogenous gastrin may be one of the releasing factors for somatostatin. Plasma levels of CCK and secretin did not change after electrical stimulation, which provides direct evidence that their release is unlikely to be under vagal control, and that CCK does not mediate the protein secretion obtained after electrical stimulation of the vagus."}
{"id": "PMID:462379", "title": "A multifactorial analysis of melanoma. II. Prognostic factors in patients with stage I (localized) melanoma.", "content": "Stage I melanoma encompasses an extraordinary diversity of biologic behavior. In such a setting where numerous parameters appear to influence survival, a multifactorial analysis using Cox's regression model is a valuable statistical model. Using a computerized data base of 394 clinical stage I melanoma patients treated at this institution during the past 20 years, a multifactorial analysis was used to compare the relative prognostic strength of 11 parameters. Two pathological factors (tumor thickness and ulceration) and two clinial factors (initial surgical treatment and anatomic location) were identified as the dominant prognostic variables. Other factors examined simultaneously that did not provide additional predictive influence on survival included the level of invasion, pigmentation, growth pattern, lymphocyte infiltration, pathological state, sex, and age. Melanoma thickness was the most important factor for predicting survival in patients with stage I melanoma (P less than 10(-8). This parameter is easy to measure and provides a quantitative estimate of clinically occult regional and distant metastases. Contrary to other reports using single factor analysis, the type of initial surgical treatment, in fact, did influence survival after other variables were taken into consideration. Thus the multifactorial analysis supports the observation that patients with intermediate thickness melanoma thickness of 1.5 to 3.99 mm had a 78% 8-year survival rate with wide excision of the melanoma and elective node dissection, while none survived more than 8 years if a melanoma of the same thickness was only widely excised. Multifactorial analysis is a useful and important statistical method when comparing treatment alternatives and prognostic factors in patients with melanoma.", "contents": "A multifactorial analysis of melanoma. II. Prognostic factors in patients with stage I (localized) melanoma. Stage I melanoma encompasses an extraordinary diversity of biologic behavior. In such a setting where numerous parameters appear to influence survival, a multifactorial analysis using Cox's regression model is a valuable statistical model. Using a computerized data base of 394 clinical stage I melanoma patients treated at this institution during the past 20 years, a multifactorial analysis was used to compare the relative prognostic strength of 11 parameters. Two pathological factors (tumor thickness and ulceration) and two clinial factors (initial surgical treatment and anatomic location) were identified as the dominant prognostic variables. Other factors examined simultaneously that did not provide additional predictive influence on survival included the level of invasion, pigmentation, growth pattern, lymphocyte infiltration, pathological state, sex, and age. Melanoma thickness was the most important factor for predicting survival in patients with stage I melanoma (P less than 10(-8). This parameter is easy to measure and provides a quantitative estimate of clinically occult regional and distant metastases. Contrary to other reports using single factor analysis, the type of initial surgical treatment, in fact, did influence survival after other variables were taken into consideration. Thus the multifactorial analysis supports the observation that patients with intermediate thickness melanoma thickness of 1.5 to 3.99 mm had a 78% 8-year survival rate with wide excision of the melanoma and elective node dissection, while none survived more than 8 years if a melanoma of the same thickness was only widely excised. Multifactorial analysis is a useful and important statistical method when comparing treatment alternatives and prognostic factors in patients with melanoma."}
{"id": "PMID:462414", "title": "In vivo effect of suloctidil as an antiplatelet agent.", "content": "The relationship between the effects of suloctidil in vivo as an antiplatelet agent and in vitro as a modifier of platelet serotonin (5-HT) parameters was investigated. Suloctidil was found to be effective in reducing platelet aggregates formation in the retired breeder rat as determined using the platelet aggregate ratio method (PAR) with an ED50 of 16.1 mg/kg 24 hours post administration. In contrast to the hypothesis that 5-HT depletion is involved in the anti-aggregatory mechanism of suloctidil, no correlation was found between platelet 5-HT content and this antiplatelet activity. Reduction of platelet 5-HT content required multiple injections of high doses (100 mg/kg/day) of suloctidil. Suloctidil administration for 8 days at 100 mg/kg/day, which lowered platelet 5-HT content by 50%, resulted in no permanent effect on ex vivo platelet 5-HT uptake or thrombin-induced release, nor alteration in the plasma 5-HT level. However, these platelets exhibited a short-lived, significant increase in percent leakage of 5-HT  after 30 minutes of incubation. Therefore, suloctidil treatment at high doses may with time result in platelet 5-HT depletion, however this effect is probably not related to the primary anti-aggregatory activity of the drug.", "contents": "In vivo effect of suloctidil as an antiplatelet agent. The relationship between the effects of suloctidil in vivo as an antiplatelet agent and in vitro as a modifier of platelet serotonin (5-HT) parameters was investigated. Suloctidil was found to be effective in reducing platelet aggregates formation in the retired breeder rat as determined using the platelet aggregate ratio method (PAR) with an ED50 of 16.1 mg/kg 24 hours post administration. In contrast to the hypothesis that 5-HT depletion is involved in the anti-aggregatory mechanism of suloctidil, no correlation was found between platelet 5-HT content and this antiplatelet activity. Reduction of platelet 5-HT content required multiple injections of high doses (100 mg/kg/day) of suloctidil. Suloctidil administration for 8 days at 100 mg/kg/day, which lowered platelet 5-HT content by 50%, resulted in no permanent effect on ex vivo platelet 5-HT uptake or thrombin-induced release, nor alteration in the plasma 5-HT level. However, these platelets exhibited a short-lived, significant increase in percent leakage of 5-HT  after 30 minutes of incubation. Therefore, suloctidil treatment at high doses may with time result in platelet 5-HT depletion, however this effect is probably not related to the primary anti-aggregatory activity of the drug."}
{"id": "PMID:462415", "title": "The action mechanism of the purified platelet aggregation principle of Trimeresurus mucrosquamatus venom.", "content": "The minimal concentration of the platelet aggregation principle (Platelet Aggregoserpentin, PAS) necessary to induce platelet aggregation was 10 ng/ml, about one-hundredth of that of the crude venom. PAS induced the release of platelet factors 3 and 4 from platelets, but the released platelet factor 3 was easily inactivated by the anti-phospholipid effect of PAS. Pretreatment of platelets with neuraminidase potentiated PAS-induced platelet aggregation. PAS-induced platelet aggregation was independent on released ADP; it could occur in the ADP-removing systems, such as apyrase or a combination of phosphoenolpyruvate and pyruvate kinase. However, PAS-induced platelet aggregation could be inhibited by adenine nucleotides and adenosine. PAS-induced platelet aggregation was inhibited by some anti-inflammatory agents, antimalarial drugs, local anesthetics, antihistamine and smooth muscle relaxants. After deaggregation of PAS-treated platelets, thrombin and sodium arachidonate could further induce platelet aggregation, but ADP and second dose of PAS could not. It is concluded that PAS-induced platelet aggregation is due to prostaglandin synthesis. Recent literatures on the mechanism of platelet aggregation were surveyed and the actions of PAS were discussed.", "contents": "The action mechanism of the purified platelet aggregation principle of Trimeresurus mucrosquamatus venom. The minimal concentration of the platelet aggregation principle (Platelet Aggregoserpentin, PAS) necessary to induce platelet aggregation was 10 ng/ml, about one-hundredth of that of the crude venom. PAS induced the release of platelet factors 3 and 4 from platelets, but the released platelet factor 3 was easily inactivated by the anti-phospholipid effect of PAS. Pretreatment of platelets with neuraminidase potentiated PAS-induced platelet aggregation. PAS-induced platelet aggregation was independent on released ADP; it could occur in the ADP-removing systems, such as apyrase or a combination of phosphoenolpyruvate and pyruvate kinase. However, PAS-induced platelet aggregation could be inhibited by adenine nucleotides and adenosine. PAS-induced platelet aggregation was inhibited by some anti-inflammatory agents, antimalarial drugs, local anesthetics, antihistamine and smooth muscle relaxants. After deaggregation of PAS-treated platelets, thrombin and sodium arachidonate could further induce platelet aggregation, but ADP and second dose of PAS could not. It is concluded that PAS-induced platelet aggregation is due to prostaglandin synthesis. Recent literatures on the mechanism of platelet aggregation were surveyed and the actions of PAS were discussed."}
{"id": "PMID:462416", "title": "Effects of adenosine diphosphate, colchicine and temperature on size of human platelets.", "content": "An improved measuring system based on the Coulter principle and developed in our laboratory is used to size human blood platelets. The mean volume of platelets in 24 healthy subjects is found to be 8.45 micron 3 with a standard deviation of 1.07 micron 3; the typical size-distribution curve is unimodal and asymmetrical, with a marked skew to the right. The effects of different reagents on platelet size (shape factor x volume) are evaluated. Platelets increase in size by 23% following suspension in isotonic, phosphate-buffered saline and incubation with 10 microM adenosine diphosphate; no change is observed when the suspending medium is autologous plasma. Cooling the platelets to 0-4 degrees C results in a size increase of 25%; rewarming to 37 degrees C restores them to their initial size within 2 hr. A similar increase occurs when the platelets are incubated with 1-10 mM colchicine. It is proposed that these reagents, which are known to produce changes in the orientation of the marginal bundle of microtubules, cause platelets to undergo disc-sphere transformations. Calculations are made which show that such transformations increase platelet size by 27% as measured electrically, and we conclude that the so-called volume changes reported in the literature reflect shape changes only and that no true volume increase actually takes place.", "contents": "Effects of adenosine diphosphate, colchicine and temperature on size of human platelets. An improved measuring system based on the Coulter principle and developed in our laboratory is used to size human blood platelets. The mean volume of platelets in 24 healthy subjects is found to be 8.45 micron 3 with a standard deviation of 1.07 micron 3; the typical size-distribution curve is unimodal and asymmetrical, with a marked skew to the right. The effects of different reagents on platelet size (shape factor x volume) are evaluated. Platelets increase in size by 23% following suspension in isotonic, phosphate-buffered saline and incubation with 10 microM adenosine diphosphate; no change is observed when the suspending medium is autologous plasma. Cooling the platelets to 0-4 degrees C results in a size increase of 25%; rewarming to 37 degrees C restores them to their initial size within 2 hr. A similar increase occurs when the platelets are incubated with 1-10 mM colchicine. It is proposed that these reagents, which are known to produce changes in the orientation of the marginal bundle of microtubules, cause platelets to undergo disc-sphere transformations. Calculations are made which show that such transformations increase platelet size by 27% as measured electrically, and we conclude that the so-called volume changes reported in the literature reflect shape changes only and that no true volume increase actually takes place."}
{"id": "PMID:462417", "title": "Direct measurement of the amount of bound collagen which stimulates platelet aggregation.", "content": "The amount of collagen which binds to platelets has been correlated with aggregating activity in a platelet aggregometer. Aggregating activity requires only 0.22 microgram/ml (0.35 microgram/10(9) cells) of collagen bound, the reaction saturating with 4.9 microgram/ml (7.9 microgram/10(9) cells). On the basis of the fraction of collagen bound and its hydroxyproline/proline ratio, it is concluded that the activity resides in the type I collagen component.", "contents": "Direct measurement of the amount of bound collagen which stimulates platelet aggregation. The amount of collagen which binds to platelets has been correlated with aggregating activity in a platelet aggregometer. Aggregating activity requires only 0.22 microgram/ml (0.35 microgram/10(9) cells) of collagen bound, the reaction saturating with 4.9 microgram/ml (7.9 microgram/10(9) cells). On the basis of the fraction of collagen bound and its hydroxyproline/proline ratio, it is concluded that the activity resides in the type I collagen component."}
{"id": "PMID:462418", "title": "Effect of factor XII deficiency on pregnancy and parturition.", "content": "The clotting parameter of a primigravida with factor XII deficiency was studied during her third trimester of pregnancy, labor and post-partum; and compared with those of her newborn male infant. Sharp increases in factors VII, VIII, IX, X and moderate increases in factors II and XI were documented during pregnancy and at labor. All factors had returned to normal or near normal levels 24 hours after delivery. Factor XII remained at 0.0 level throughout. In the infant the clotting factor levels reflected depression of vitamins K-dependent factors II, IX, and X and a factor XII level of 40.0%. No undue bleeding was noted in the mother at delivery or placental separation, and no bleeding manifestation was apparent in the infant. These findings suggest that factor XII does not play a major role in triggering or modulating the course of normal labor, nor is its absence necessarily associated with bleeding complications during parturition or placental separation.", "contents": "Effect of factor XII deficiency on pregnancy and parturition. The clotting parameter of a primigravida with factor XII deficiency was studied during her third trimester of pregnancy, labor and post-partum; and compared with those of her newborn male infant. Sharp increases in factors VII, VIII, IX, X and moderate increases in factors II and XI were documented during pregnancy and at labor. All factors had returned to normal or near normal levels 24 hours after delivery. Factor XII remained at 0.0 level throughout. In the infant the clotting factor levels reflected depression of vitamins K-dependent factors II, IX, and X and a factor XII level of 40.0%. No undue bleeding was noted in the mother at delivery or placental separation, and no bleeding manifestation was apparent in the infant. These findings suggest that factor XII does not play a major role in triggering or modulating the course of normal labor, nor is its absence necessarily associated with bleeding complications during parturition or placental separation."}
{"id": "PMID:462419", "title": "Studies on a subcellular system for vitamin K-dependent carboxylation.", "content": "The subcellular localization of acarboxyprothrombin and the vitamin K-dependent carboxylation system has been studied using livers from vitamin K-deficient rats and found to be mainly in the membranes of the rough microsomal fraction. Bovine acarboxyprothrombin or fragment 1 thereof were inactive as substrates in the rat carboxylating system.", "contents": "Studies on a subcellular system for vitamin K-dependent carboxylation. The subcellular localization of acarboxyprothrombin and the vitamin K-dependent carboxylation system has been studied using livers from vitamin K-deficient rats and found to be mainly in the membranes of the rough microsomal fraction. Bovine acarboxyprothrombin or fragment 1 thereof were inactive as substrates in the rat carboxylating system."}
{"id": "PMID:462420", "title": "Prevention of intravascular thrombus formation on plastic catheters with heparin-benzalkonium complex: in vivo and in vitro studies.", "content": "A complex of heparin and a quaternary ammonium compound dissolved in an organic solvent was investigated for its potential ability to prevent clotting on the surface of plastic catheters. Despite the complexing of heparin to ammonium, anticoagulant activity remained on in vitro testing. Both the heparin complex and its organic solvent alone partially prevented catheter thrombus formation when tested in an in vivo animal model system. Neither, however, was totally effective in preventing thrombosis.", "contents": "Prevention of intravascular thrombus formation on plastic catheters with heparin-benzalkonium complex: in vivo and in vitro studies. A complex of heparin and a quaternary ammonium compound dissolved in an organic solvent was investigated for its potential ability to prevent clotting on the surface of plastic catheters. Despite the complexing of heparin to ammonium, anticoagulant activity remained on in vitro testing. Both the heparin complex and its organic solvent alone partially prevented catheter thrombus formation when tested in an in vivo animal model system. Neither, however, was totally effective in preventing thrombosis."}
{"id": "PMID:462421", "title": "Plasma thrombin assay using a chromogenic substrate in disseminated intravascular coagulation due to snake bite envenomation.", "content": "Using the chromogenic substrate, Tos-Gly-Pro-Arg-pNA-HCl (Chromozym TH, Boehringer Mannheim) plasma thrombin was estimated in six cases of envenomation by Australian elapid snakes. All patients manifested findings characteristic of defibrination due to envenomation by these snakes. Fibrin-fibrinogen degradation products were grossly elevated, as was plasma thrombin in all cases. Following treatment with antivenene, all abnormal coagulation parameters returned rapidly towards normal by 24 hours and plasma thrombin disappeared.", "contents": "Plasma thrombin assay using a chromogenic substrate in disseminated intravascular coagulation due to snake bite envenomation. Using the chromogenic substrate, Tos-Gly-Pro-Arg-pNA-HCl (Chromozym TH, Boehringer Mannheim) plasma thrombin was estimated in six cases of envenomation by Australian elapid snakes. All patients manifested findings characteristic of defibrination due to envenomation by these snakes. Fibrin-fibrinogen degradation products were grossly elevated, as was plasma thrombin in all cases. Following treatment with antivenene, all abnormal coagulation parameters returned rapidly towards normal by 24 hours and plasma thrombin disappeared."}
{"id": "PMID:462422", "title": "Detection and partial purification of a natural heparin inhibitor from hog small intestine.", "content": "A natural occurring heparin inhibitor was detected and was partially purified from the mucosa of hog small intestine. The mucosa was homogenized and was extracted overnight in 0.15 M NaCl, 0.01 M imidazole, 0.001 M EDTA, pH 6.5. When the extract was made to 85% saturation in ammonium sulfate, a large quantity of heparin neutralizing activity was detected in the precipitate. Each small intestine contains approximately 35,000 units of heparin neutralizing activity. This heparin inhibitor was further purified by the procedures of zinc sulfate precipitation, ammonium sulfate fractionation, ethanol precipitaiton and heparin-sepharose chromatography. A 37 fold partial purification with 15% overall recovery was achieved to yield heparin inhibitor with specific activity of 50-65 units per mg of protein.", "contents": "Detection and partial purification of a natural heparin inhibitor from hog small intestine. A natural occurring heparin inhibitor was detected and was partially purified from the mucosa of hog small intestine. The mucosa was homogenized and was extracted overnight in 0.15 M NaCl, 0.01 M imidazole, 0.001 M EDTA, pH 6.5. When the extract was made to 85% saturation in ammonium sulfate, a large quantity of heparin neutralizing activity was detected in the precipitate. Each small intestine contains approximately 35,000 units of heparin neutralizing activity. This heparin inhibitor was further purified by the procedures of zinc sulfate precipitation, ammonium sulfate fractionation, ethanol precipitaiton and heparin-sepharose chromatography. A 37 fold partial purification with 15% overall recovery was achieved to yield heparin inhibitor with specific activity of 50-65 units per mg of protein."}
{"id": "PMID:462423", "title": "A in vivo assay for standardizing heparin preparations.", "content": "The limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use. The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a \"2 and 2 dose assay\" procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation. The similarity of results obtained in vivo method and in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.", "contents": "A in vivo assay for standardizing heparin preparations. The limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use. The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a \"2 and 2 dose assay\" procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation. The similarity of results obtained in vivo method and in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin."}
{"id": "PMID:462424", "title": "Measurement of the heparin neutralizing capacity of protamine.", "content": "Several methods of estimating the heparin neutralizing capacity of protamine were investigated for their reliability and practicability. The results from two chemical methods were compared with those from two in vitro biological assays, one of which was the method of the British Pharmacopoeia (1973). An in vivo method using mice was used to assess the accuracy of the in vitro test methods. Three standard heparin preparations were tested against the W.H.O. 1st International Reference Preparation for Protamine. Two of the heparin preparations were of mucosal origin, one of which was the 3rd International Standard, and the third heparin preparation was of lung origin (the 2nd International Standard for Heparin). The mean neutralization values (all methods) of heparin by protamine for a House Reference Preparation and the 3rd and 2nd International Standards for Heparin were 95.8, 109.8 and 89.9 units per mg of Protamine, respectively. All methods read the House Reference Preparation to have a lower value than the 3rd International Standard, which had a higher value than the 2nd International Standard for Heparin. There was a constant relationship between the results of any one method and those of another. The chemical and in vitro biological methods gave results of comparable precision although the latter required a greater degree of technical skill and time to perform.", "contents": "Measurement of the heparin neutralizing capacity of protamine. Several methods of estimating the heparin neutralizing capacity of protamine were investigated for their reliability and practicability. The results from two chemical methods were compared with those from two in vitro biological assays, one of which was the method of the British Pharmacopoeia (1973). An in vivo method using mice was used to assess the accuracy of the in vitro test methods. Three standard heparin preparations were tested against the W.H.O. 1st International Reference Preparation for Protamine. Two of the heparin preparations were of mucosal origin, one of which was the 3rd International Standard, and the third heparin preparation was of lung origin (the 2nd International Standard for Heparin). The mean neutralization values (all methods) of heparin by protamine for a House Reference Preparation and the 3rd and 2nd International Standards for Heparin were 95.8, 109.8 and 89.9 units per mg of Protamine, respectively. All methods read the House Reference Preparation to have a lower value than the 3rd International Standard, which had a higher value than the 2nd International Standard for Heparin. There was a constant relationship between the results of any one method and those of another. The chemical and in vitro biological methods gave results of comparable precision although the latter required a greater degree of technical skill and time to perform."}
{"id": "PMID:462425", "title": "Somatostatin-induced changes in the hemostasis of rats.", "content": "Somatostatin (SRIF) given intravenously, either as a single bolus or as a 2 hr infusion caused a significant prolongation of partial thromboplastin time (PTT) and depressed platelet counts and platelet aggregation in the rat. Following daily injections of protamin-zinc SRIF for 2 weeks the platelet count returned to normal, PTT remained prolonged and platelet aggregation was enhanced. The doses of SRIF used in this work were adequate to suppress the secretion of insulin and glucagon by the isolated pancreatic islets of treated animals.", "contents": "Somatostatin-induced changes in the hemostasis of rats. Somatostatin (SRIF) given intravenously, either as a single bolus or as a 2 hr infusion caused a significant prolongation of partial thromboplastin time (PTT) and depressed platelet counts and platelet aggregation in the rat. Following daily injections of protamin-zinc SRIF for 2 weeks the platelet count returned to normal, PTT remained prolonged and platelet aggregation was enhanced. The doses of SRIF used in this work were adequate to suppress the secretion of insulin and glucagon by the isolated pancreatic islets of treated animals."}
{"id": "PMID:462426", "title": "The nucleated thrombocytoid cells. I. Electron microscopic studies on chicken blood cells.", "content": "By analyses of electron micrographs it was investigated whether nucleated thrombocytes of chickens described in the literature can be differentiated from lymphoid cells with sufficient certainty as an independent cell system. The result was negative. The mixture of lymphoid cells and so-called thrombocytes investigated was extremely polymorphous and, unlike mammalian platelets, proved to be one cell system containing younger and more mature cells. In the literature cells with signs of overmaturity or activation are designated as thrombocytes. Our studies suggest that a genuine thrombocyte system of chickens and other birds is not existent. The cell hitherto considered as nucleated thrombocytes seem to belong to the lymphoid system.", "contents": "The nucleated thrombocytoid cells. I. Electron microscopic studies on chicken blood cells. By analyses of electron micrographs it was investigated whether nucleated thrombocytes of chickens described in the literature can be differentiated from lymphoid cells with sufficient certainty as an independent cell system. The result was negative. The mixture of lymphoid cells and so-called thrombocytes investigated was extremely polymorphous and, unlike mammalian platelets, proved to be one cell system containing younger and more mature cells. In the literature cells with signs of overmaturity or activation are designated as thrombocytes. Our studies suggest that a genuine thrombocyte system of chickens and other birds is not existent. The cell hitherto considered as nucleated thrombocytes seem to belong to the lymphoid system."}
{"id": "PMID:462455", "title": "[Bone tumours in the shoulder area (author's transl)].", "content": "An introduction into the basic principles of radiographic interpretation of bone tumours is presented. Patient material is selected in order to demonstrate the different appearances of bone tumours. The basic patterns of periosteal response are discussed. A short discussion of differential diagnosis is included.", "contents": "[Bone tumours in the shoulder area (author's transl)]. An introduction into the basic principles of radiographic interpretation of bone tumours is presented. Patient material is selected in order to demonstrate the different appearances of bone tumours. The basic patterns of periosteal response are discussed. A short discussion of differential diagnosis is included."}
{"id": "PMID:462456", "title": "[Mycoplasma synoviae control. II. Treatment of hatching eggs with tylosin tartrate (author's transl)].", "content": "Several strains of Mycoplasma synoviae isolated in the Netherlands during the past five years, were found to be sensitive to tylosin tartrate in vitro as well as in vivo. In one of four cases in which laying hens were infected experimentally, vertical transmission of the infective agent was demonstrated by the isolation of Mycoplasma synoviae from hatching eggs laid during the second and third weeks after infection. Treatment of experimentally infected eggs and eggs laid by infected flocks with 2.5 mg. of tylosin tartrate in the aircell on the ninth day of incubation resulted in the elimination of Mycoplasma synoviae. This procedure also underwent large-scale testing in the field. Injection of 0.8 and 1.5 mg. of tylosin tartrate into the albumen of non-incubated chicken eggs or turkey eggs through an area at a distance of approximately 1 cm. from the tapering extremity will probably provide an attractive alternative to egg-dipping in the control of mycoplasma infection.", "contents": "[Mycoplasma synoviae control. II. Treatment of hatching eggs with tylosin tartrate (author's transl)]. Several strains of Mycoplasma synoviae isolated in the Netherlands during the past five years, were found to be sensitive to tylosin tartrate in vitro as well as in vivo. In one of four cases in which laying hens were infected experimentally, vertical transmission of the infective agent was demonstrated by the isolation of Mycoplasma synoviae from hatching eggs laid during the second and third weeks after infection. Treatment of experimentally infected eggs and eggs laid by infected flocks with 2.5 mg. of tylosin tartrate in the aircell on the ninth day of incubation resulted in the elimination of Mycoplasma synoviae. This procedure also underwent large-scale testing in the field. Injection of 0.8 and 1.5 mg. of tylosin tartrate into the albumen of non-incubated chicken eggs or turkey eggs through an area at a distance of approximately 1 cm. from the tapering extremity will probably provide an attractive alternative to egg-dipping in the control of mycoplasma infection."}
{"id": "PMID:462458", "title": "[Haemoglobin and haematocrit levels in veal calves (author's transl)].", "content": "The haemoglobin and haematocrit levels were studied in three groups of about thirty male Dutch-Friesian veal calves. The iron content of the milk replaces was 5, 10 and 25 ppm. During the experiment which was continued for sixteen weeks, the haemoglobin and haematocrit levels decreased in all groups. The decrease was confined to the first six weeks in the calves fed the milk replacer containing 25 ppm of iron. In the two other groups, the decrease of the two criteria continued throuhgout period. Regardless of the iron content of the milk replacer, the decrease of Hb- and haematocrit levels of the individual calves was greater in those having the highest initial level. The borderline for anaemia in a group of sixteen-week-old veal calves is estimated at approximately 10 g. Hb/100 ml. blood. For an individual calf, the borderline is estimated at 8 g. Hb/100 ml. blood.", "contents": "[Haemoglobin and haematocrit levels in veal calves (author's transl)]. The haemoglobin and haematocrit levels were studied in three groups of about thirty male Dutch-Friesian veal calves. The iron content of the milk replaces was 5, 10 and 25 ppm. During the experiment which was continued for sixteen weeks, the haemoglobin and haematocrit levels decreased in all groups. The decrease was confined to the first six weeks in the calves fed the milk replacer containing 25 ppm of iron. In the two other groups, the decrease of the two criteria continued throuhgout period. Regardless of the iron content of the milk replacer, the decrease of Hb- and haematocrit levels of the individual calves was greater in those having the highest initial level. The borderline for anaemia in a group of sixteen-week-old veal calves is estimated at approximately 10 g. Hb/100 ml. blood. For an individual calf, the borderline is estimated at 8 g. Hb/100 ml. blood."}
{"id": "PMID:462459", "title": "[Registering, licensing and investigating vaccines in animal disease control (author's transl)].", "content": "The registration, licensing and investigation of veterinary vaccines are discussed with reference to the producer, the user and the authorities. The significance of basic research by the producer, of testing by the authorities, of quality control and of evaluation (laboratory and field studies) in the control of animal diseases are examined more closely. The fact is stressed, that legislation concerning veterinary medicines in general and vaccines in particular should be well defined. All this is regarded as an integral part of recent developments in animal farming in the Netherlands.", "contents": "[Registering, licensing and investigating vaccines in animal disease control (author's transl)]. The registration, licensing and investigation of veterinary vaccines are discussed with reference to the producer, the user and the authorities. The significance of basic research by the producer, of testing by the authorities, of quality control and of evaluation (laboratory and field studies) in the control of animal diseases are examined more closely. The fact is stressed, that legislation concerning veterinary medicines in general and vaccines in particular should be well defined. All this is regarded as an integral part of recent developments in animal farming in the Netherlands."}
{"id": "PMID:462462", "title": "Recovery of the heart after normothermic ischemia. Part II: Myocardial function during postischemic reperfusion.", "content": "Contraction and relaxation of the canine myocardium were examined during normothermic ischemia in an isolated heart model. Decrease in the development of tension depends on the duration of ischemia. Deficient functional recovery was observed after ischemic periods extending beyond 30 minutes, in spite of reperfusion periods of over 1 hour. A decrease in compliance was observed during the anoxic period, but a persistent defect of relaxation occurred only after 60 minutes of ischemia. After this period there was also a disturbance in the autoregulative mechanisms of coronary perfusion and an uncoupling of O2-consumption and mechanical efficiency. A prolonged reperfusion period of the heart beating empty allowed ultrastructural recovery of the damaged myocardium. In contrast, functional recovery of the myocardium, as determined by several parameters of contraction and relaxation, did not correlate with ultrastructural recovery and was not improved by prolonged reperfusion.", "contents": "Recovery of the heart after normothermic ischemia. Part II: Myocardial function during postischemic reperfusion. Contraction and relaxation of the canine myocardium were examined during normothermic ischemia in an isolated heart model. Decrease in the development of tension depends on the duration of ischemia. Deficient functional recovery was observed after ischemic periods extending beyond 30 minutes, in spite of reperfusion periods of over 1 hour. A decrease in compliance was observed during the anoxic period, but a persistent defect of relaxation occurred only after 60 minutes of ischemia. After this period there was also a disturbance in the autoregulative mechanisms of coronary perfusion and an uncoupling of O2-consumption and mechanical efficiency. A prolonged reperfusion period of the heart beating empty allowed ultrastructural recovery of the damaged myocardium. In contrast, functional recovery of the myocardium, as determined by several parameters of contraction and relaxation, did not correlate with ultrastructural recovery and was not improved by prolonged reperfusion."}
{"id": "PMID:462463", "title": "Inotropic agents and functional recovery from ischemic cardiac arrest.", "content": "Short periods of ischemic arrest may cause myocardial cell damage so that reperfusion does not result in an immediate return to normal metabolic and contractile activity. Studies have been carried out using a rat heart model of cardiopulmonary bypass and ischemic cardiac arrest in order to determine whether the use of inotropic agents (isoprenaline and dobutamine) to augment contractile activity during the early phase of post-ischemic reperfusion extends pre-existing damage and results in ultimate impairment of contractile activity. The results indicated the existance of a substantial metabolic reserve, capable of providing sufficient energy for a marked increase in dP/dt and heart rate, without inducing tissue damage. However, in this isolated rat heart preparation the use of these agents had relatively little effect upon cardiac output and could be shown to induce a dose-dependent reduction in stroke volume.", "contents": "Inotropic agents and functional recovery from ischemic cardiac arrest. Short periods of ischemic arrest may cause myocardial cell damage so that reperfusion does not result in an immediate return to normal metabolic and contractile activity. Studies have been carried out using a rat heart model of cardiopulmonary bypass and ischemic cardiac arrest in order to determine whether the use of inotropic agents (isoprenaline and dobutamine) to augment contractile activity during the early phase of post-ischemic reperfusion extends pre-existing damage and results in ultimate impairment of contractile activity. The results indicated the existance of a substantial metabolic reserve, capable of providing sufficient energy for a marked increase in dP/dt and heart rate, without inducing tissue damage. However, in this isolated rat heart preparation the use of these agents had relatively little effect upon cardiac output and could be shown to induce a dose-dependent reduction in stroke volume."}
{"id": "PMID:462464", "title": "Critical pulmonary valve stenosis with intact ventricular septum.", "content": "This study presents our experience in the surgical management of the newborn with critical pulmonary stenosis and intact ventricular septum. During the last decade we have seen 11 such patients, with a mean age of 2.3 days (maximum 6 days) and a mean weight of 3.6 kilograms (maximum 4.5 kilograms). The primary operation was a valvotomy in 7 patients (one of whom also had a systemic-pulmonary shunt), a commissurotomy with cardiopulmonary bypass in 3 patients, and a shunt alone in 1 patient. One infant died on the second postoperative day and another died following reoperation at 4 months. The other 9 patients were all alive and progressing satisfactorily when last seen. Five of these patients were beyond their third postoperative year and the longest survivor was at 7.9 years. Four of the long-term survivors had undergone reoperation. Our choice of operation remains transventricular valvotomy, although an open procedure utilizing cardiopulmonary bypass presents an attractive alternative. A systemic-pulmonary shunt as an addition to either of these operations is unnecessary. The perioperative use of prostaglandin E brings about substantial improvement in the early results.", "contents": "Critical pulmonary valve stenosis with intact ventricular septum. This study presents our experience in the surgical management of the newborn with critical pulmonary stenosis and intact ventricular septum. During the last decade we have seen 11 such patients, with a mean age of 2.3 days (maximum 6 days) and a mean weight of 3.6 kilograms (maximum 4.5 kilograms). The primary operation was a valvotomy in 7 patients (one of whom also had a systemic-pulmonary shunt), a commissurotomy with cardiopulmonary bypass in 3 patients, and a shunt alone in 1 patient. One infant died on the second postoperative day and another died following reoperation at 4 months. The other 9 patients were all alive and progressing satisfactorily when last seen. Five of these patients were beyond their third postoperative year and the longest survivor was at 7.9 years. Four of the long-term survivors had undergone reoperation. Our choice of operation remains transventricular valvotomy, although an open procedure utilizing cardiopulmonary bypass presents an attractive alternative. A systemic-pulmonary shunt as an addition to either of these operations is unnecessary. The perioperative use of prostaglandin E brings about substantial improvement in the early results."}
{"id": "PMID:462465", "title": "Long-term observation of pacemaker electrodes. A follow-up study of 328 consecutive patients treated between 1959 and 1967.", "content": "Epicardial electrodes implanted in 20 patients between 1959 and 1962 had a short-term effectiveness. A change to endocardial stimulation was later necessary in 15 of these patients. Endocardial electrodes inserted between 1962 and 1967 in 306 patients were included in this study; 121 (40%) of these patients survived for 10 years or more. Seventy-five (62%) of the long-term survivors had no problems with their electrodes. In 39 of these patients the stimulation thresholds were measured after both 5 and 10 years and found to be 2.3 +/- 0.6 volts and 2.2 +/- 0.8 volts, respectively. Hence, long-term complication-free endocardial pacing can be accomplished.", "contents": "Long-term observation of pacemaker electrodes. A follow-up study of 328 consecutive patients treated between 1959 and 1967. Epicardial electrodes implanted in 20 patients between 1959 and 1962 had a short-term effectiveness. A change to endocardial stimulation was later necessary in 15 of these patients. Endocardial electrodes inserted between 1962 and 1967 in 306 patients were included in this study; 121 (40%) of these patients survived for 10 years or more. Seventy-five (62%) of the long-term survivors had no problems with their electrodes. In 39 of these patients the stimulation thresholds were measured after both 5 and 10 years and found to be 2.3 +/- 0.6 volts and 2.2 +/- 0.8 volts, respectively. Hence, long-term complication-free endocardial pacing can be accomplished."}
{"id": "PMID:462466", "title": "The effects of deep hypothermia and circulatory arrest on systemic metabolic state of infants undergoing corrective open heart surgery: a comparison of two methods.", "content": "Fortyone of 187 infants undergoing corrective surgery for their congenital cardiac lesions using profound hypothermic circulatory arrest were randomly selected for metabolic studies. Deep hypothermia of 21 to 22 degrees C core temperature was reached by two different techniques: 1. Perfusion cooling by extracorporeal circulation (ECC-C) 2. Surface cooling with ice bags combined with perfusion cooling (SC + ECC-C) After circulatory arrest (34.2 min. ECC-C v.s. 46.7 min. SC + ECC-C) bypass rewarming was used in both groups. The metabolic reaction to these interventions are described. No significant differences in acid base status in oxygen consumption, lactate concentration, serum electrolytes (K+, Na+, Ca++,Cl-) and serum enzyme activity (CPK, alpha-HBDH, LDH, SGOT, SGPT) could be demonstrated between the two groups of patients during the entire course of cooling, circulatory arrest and rewarming. The glucose concentration was significantly lower in the ECC-C group during the entire period of operation. Total cooling time was significantly shorter in the group without surface cooling. (ECC-C: 12 min, v.s. SC + ECC-C: 64 min). Since no favourable effects of the SC + ECC-C method on systemic metabolism could be demonstrated and operative results were similar we now prefer the time-saving ECC-C technique.", "contents": "The effects of deep hypothermia and circulatory arrest on systemic metabolic state of infants undergoing corrective open heart surgery: a comparison of two methods. Fortyone of 187 infants undergoing corrective surgery for their congenital cardiac lesions using profound hypothermic circulatory arrest were randomly selected for metabolic studies. Deep hypothermia of 21 to 22 degrees C core temperature was reached by two different techniques: 1. Perfusion cooling by extracorporeal circulation (ECC-C) 2. Surface cooling with ice bags combined with perfusion cooling (SC + ECC-C) After circulatory arrest (34.2 min. ECC-C v.s. 46.7 min. SC + ECC-C) bypass rewarming was used in both groups. The metabolic reaction to these interventions are described. No significant differences in acid base status in oxygen consumption, lactate concentration, serum electrolytes (K+, Na+, Ca++,Cl-) and serum enzyme activity (CPK, alpha-HBDH, LDH, SGOT, SGPT) could be demonstrated between the two groups of patients during the entire course of cooling, circulatory arrest and rewarming. The glucose concentration was significantly lower in the ECC-C group during the entire period of operation. Total cooling time was significantly shorter in the group without surface cooling. (ECC-C: 12 min, v.s. SC + ECC-C: 64 min). Since no favourable effects of the SC + ECC-C method on systemic metabolism could be demonstrated and operative results were similar we now prefer the time-saving ECC-C technique."}
{"id": "PMID:462468", "title": "Heart valve replacement with the St. Jude medical prosthesis -- first clinical results --.", "content": "Starting in February 1978 52 St. Jude Medical Prostheses have been implanted in 47 patients (age 13--73 years) in aortic, mitral and tricuspid positions. Perioperative death occurred in 3 cases. Post mortem examination of these patients showed unimpaired valvular function. There were no other complications in an accumulated total valve time of approximately 300 months. Prior to surgery and post-operatively, several hemolytic parameters were analyzed. There was only a mild elevation in Serum LDH (285 +/- 10 iV/L). Fifteen patients underwent recatheterization 3 months postoperatively. The mitral valves did not reveal an enddiastolic gradient at rest, while the aortic valves had a peak to peak gradient of 8 +/- 2.1 mmHg. A standardized stress consisting of an infusion of isoproterenol was used. The results were compared to results obtained in patients with Bj\u00f6rk-Shiley prostheses under identical conditions and did not show a significant difference. The preliminary hemodynamic findings correlate with preceding in vitro results and preclinical data, and the first clinical experience is promising.", "contents": "Heart valve replacement with the St. Jude medical prosthesis -- first clinical results --. Starting in February 1978 52 St. Jude Medical Prostheses have been implanted in 47 patients (age 13--73 years) in aortic, mitral and tricuspid positions. Perioperative death occurred in 3 cases. Post mortem examination of these patients showed unimpaired valvular function. There were no other complications in an accumulated total valve time of approximately 300 months. Prior to surgery and post-operatively, several hemolytic parameters were analyzed. There was only a mild elevation in Serum LDH (285 +/- 10 iV/L). Fifteen patients underwent recatheterization 3 months postoperatively. The mitral valves did not reveal an enddiastolic gradient at rest, while the aortic valves had a peak to peak gradient of 8 +/- 2.1 mmHg. A standardized stress consisting of an infusion of isoproterenol was used. The results were compared to results obtained in patients with Bj\u00f6rk-Shiley prostheses under identical conditions and did not show a significant difference. The preliminary hemodynamic findings correlate with preceding in vitro results and preclinical data, and the first clinical experience is promising."}
{"id": "PMID:462469", "title": "Management of extensive lesions of the lower trachea using emergency cardiopulmonary bypass.", "content": "Rapid cardiopulmonary deterioration in patients with extensive lesions of the lower trachea presents a serious challenge in the emergency care. Conventional methods may prove inadequate to achieve airway control and to stablilize the critical condition. Severe airway obstruction and/or massive air leakage are the main causes of failure. An aggressive operative approach is mandatory. Institution of emergency cardiopulmonary bypass for resuscitative purposes and definitive surgical repair of those life-threatening uncommon tracheal lesions seems essential to us. Two cases, managed according to this concept, are presented.", "contents": "Management of extensive lesions of the lower trachea using emergency cardiopulmonary bypass. Rapid cardiopulmonary deterioration in patients with extensive lesions of the lower trachea presents a serious challenge in the emergency care. Conventional methods may prove inadequate to achieve airway control and to stablilize the critical condition. Severe airway obstruction and/or massive air leakage are the main causes of failure. An aggressive operative approach is mandatory. Institution of emergency cardiopulmonary bypass for resuscitative purposes and definitive surgical repair of those life-threatening uncommon tracheal lesions seems essential to us. Two cases, managed according to this concept, are presented."}
{"id": "PMID:462470", "title": "HLA compatibility in couples with children suffering from acute leukemia or aplastic anemia.", "content": "The frequency of common HLA-A and -B antigens were determined in 30 couples having a child with acute leukemia (AL), 34 couples having a child with aplastic anemia (AA) and 58 random couples with healthy children. An increased frequency of couples sharing at least two common antigens was observed in parents of both AL and AA children.", "contents": "HLA compatibility in couples with children suffering from acute leukemia or aplastic anemia. The frequency of common HLA-A and -B antigens were determined in 30 couples having a child with acute leukemia (AL), 34 couples having a child with aplastic anemia (AA) and 58 random couples with healthy children. An increased frequency of couples sharing at least two common antigens was observed in parents of both AL and AA children."}
{"id": "PMID:462471", "title": "Responsiveness to insulin of glucose metabolism in cultured rat and human arterial smooth muscle cells.", "content": "Smooth muscle cells growing in the primary culture derived from outgrowths of the intimal-medial explants of both rat and human arteries were used. The 72-hr sequential glucose uptake by the cells of both species in culture dishes was enhanced only slightly with time by the addition of insulin to culture medium, and this enhancement was statistically not significant. The glucose conversions to CO2  and lipids by the rat and human cells dissociated for tracer study were not affected significantly during the 2-hr incubation by the insulin addition in vitro. The smooth muscle cells of both species cultured for a week in medium enriched with insulin and then dissociated revealed the significantly increased glucose conversion to lipids, while the increase in the glucose coversion to CO2 was not significant in these cells. Thus, the smooth muscle cells of both rat and human seem to show significant metabolic response to chronic, but not acute, exposure to insulin. Therefore, it is likely that the persistent change in the insulin level may lead to abnormal metabolic state in the artery.", "contents": "Responsiveness to insulin of glucose metabolism in cultured rat and human arterial smooth muscle cells. Smooth muscle cells growing in the primary culture derived from outgrowths of the intimal-medial explants of both rat and human arteries were used. The 72-hr sequential glucose uptake by the cells of both species in culture dishes was enhanced only slightly with time by the addition of insulin to culture medium, and this enhancement was statistically not significant. The glucose conversions to CO2  and lipids by the rat and human cells dissociated for tracer study were not affected significantly during the 2-hr incubation by the insulin addition in vitro. The smooth muscle cells of both species cultured for a week in medium enriched with insulin and then dissociated revealed the significantly increased glucose conversion to lipids, while the increase in the glucose coversion to CO2 was not significant in these cells. Thus, the smooth muscle cells of both rat and human seem to show significant metabolic response to chronic, but not acute, exposure to insulin. Therefore, it is likely that the persistent change in the insulin level may lead to abnormal metabolic state in the artery."}
{"id": "PMID:462472", "title": "Bronchogenic carcinoma producing ectopic steroid hormones.", "content": "A patient with steroid hormones or androgen producing bronchogenic adenocarcinoma was presented. Clinically he had a mediastinal mass and bilateral multiple pulmonary nodules which showed a rapid growth despite cancer chemotherapy. At postmortem examination, radioimmunoassay of the tumor tissue revealed androgen and their precursors. The mitochondria of the tumor cells resembled those of cells in the zona reticularis of the adrenal cortex.", "contents": "Bronchogenic carcinoma producing ectopic steroid hormones. A patient with steroid hormones or androgen producing bronchogenic adenocarcinoma was presented. Clinically he had a mediastinal mass and bilateral multiple pulmonary nodules which showed a rapid growth despite cancer chemotherapy. At postmortem examination, radioimmunoassay of the tumor tissue revealed androgen and their precursors. The mitochondria of the tumor cells resembled those of cells in the zona reticularis of the adrenal cortex."}
{"id": "PMID:462473", "title": "The use of the anti-Acholeplasma activities of Streptomyces culture filtrates for the screening of new antibiotics.", "content": "A screening method for new antimicrobial agents from streptomyces culture filtrates was developed. Three different types of test organisms were used for determining the antimicrobial activities of the culture filtrates: they were (a) an antibacterial activity test against Staphylococcus aureus, (b) an antimycoplasmal activity test against Acholeplasma laidlawii, and (c) a cytotoxicity test against HeLa-S3 cells. The active filtrates showing antimicrobial spectra (anticellogram) which did not match those of known antimicrobial agents were used for further purification to obtain new antimicrobial agents. Of these screening methods, antimycoplasmal activity against Acholeplasma laidawii was the most sensitive for discriminating new compounds contained in the filtrates of known antibiotics.", "contents": "The use of the anti-Acholeplasma activities of Streptomyces culture filtrates for the screening of new antibiotics. A screening method for new antimicrobial agents from streptomyces culture filtrates was developed. Three different types of test organisms were used for determining the antimicrobial activities of the culture filtrates: they were (a) an antibacterial activity test against Staphylococcus aureus, (b) an antimycoplasmal activity test against Acholeplasma laidlawii, and (c) a cytotoxicity test against HeLa-S3 cells. The active filtrates showing antimicrobial spectra (anticellogram) which did not match those of known antimicrobial agents were used for further purification to obtain new antimicrobial agents. Of these screening methods, antimycoplasmal activity against Acholeplasma laidawii was the most sensitive for discriminating new compounds contained in the filtrates of known antibiotics."}
{"id": "PMID:462475", "title": "Long-term observation of arachnoid villi after subarachnoid hemorrhage: an electron microscopic study in dogs.", "content": "The sequential electron microscopic examinations were carried out on arachnoid villi of the dog after subarachnoid hemorrhage (SAH). A few days after SAH, red blood cells were accumulated and degenerated in the villi. 10 days after SAH, remarkable phagocytosis and micropinocytosis were observed in the arachnoid villi, which may suggest increased metabolic activity of the arachnoid cells. 30 days after SAH, increased cellularity in the arachnoid villi developed and intercellular spaces became very narrow. 90 days after SAH, the arachnoid villi demonstrated many cytoplasmic filaments and hemidesmosome-like structures in the arachnoid cells, as well as narrowed intercellular space and increased cellularity. Microfibrils increased and structures resembling basal laminae were observed in the stromas. These morphological alterations may have a relation to the disturbance of absorption of cerebrospinal fluid.", "contents": "Long-term observation of arachnoid villi after subarachnoid hemorrhage: an electron microscopic study in dogs. The sequential electron microscopic examinations were carried out on arachnoid villi of the dog after subarachnoid hemorrhage (SAH). A few days after SAH, red blood cells were accumulated and degenerated in the villi. 10 days after SAH, remarkable phagocytosis and micropinocytosis were observed in the arachnoid villi, which may suggest increased metabolic activity of the arachnoid cells. 30 days after SAH, increased cellularity in the arachnoid villi developed and intercellular spaces became very narrow. 90 days after SAH, the arachnoid villi demonstrated many cytoplasmic filaments and hemidesmosome-like structures in the arachnoid cells, as well as narrowed intercellular space and increased cellularity. Microfibrils increased and structures resembling basal laminae were observed in the stromas. These morphological alterations may have a relation to the disturbance of absorption of cerebrospinal fluid."}
{"id": "PMID:462477", "title": "Disintegration of urinary calculi by laser beam: drilling experiment in extracted urinary stones.", "content": "Disintegration of urinary calculi was attempted by the use of laser beam. As a first step, drilling of extracted urinary stones was attempted using a continuous wave CO2 laser and a pulse ruby laser. Stones were drilled easily by either laser beam. The power around 10 W of continuous CO2 laser beam was sufficient to drill through the stone.", "contents": "Disintegration of urinary calculi by laser beam: drilling experiment in extracted urinary stones. Disintegration of urinary calculi was attempted by the use of laser beam. As a first step, drilling of extracted urinary stones was attempted using a continuous wave CO2 laser and a pulse ruby laser. Stones were drilled easily by either laser beam. The power around 10 W of continuous CO2 laser beam was sufficient to drill through the stone."}
{"id": "PMID:462478", "title": "Direct evidence for recurrent inhibition in sliced brain preparation of the cat's visual cortex.", "content": "To reveal the recurrent inhibitory circuit in the visual cortex, a depolarizing current was applied through a glass microelectrode to an impaled cell in an in vitro slice of the visual cortex obtained from a cat anesthetized with pentobarbital. The cell reported here produced inhibitory postsynaptic potentials (IPSPs) following single spikes or bursts of spikes which were elicited by intracellularly applied current. This observation indicates that IPSPs have been mediated by the recurrent inhibitory circuit via axon collaterals of the impaled cell.", "contents": "Direct evidence for recurrent inhibition in sliced brain preparation of the cat's visual cortex. To reveal the recurrent inhibitory circuit in the visual cortex, a depolarizing current was applied through a glass microelectrode to an impaled cell in an in vitro slice of the visual cortex obtained from a cat anesthetized with pentobarbital. The cell reported here produced inhibitory postsynaptic potentials (IPSPs) following single spikes or bursts of spikes which were elicited by intracellularly applied current. This observation indicates that IPSPs have been mediated by the recurrent inhibitory circuit via axon collaterals of the impaled cell."}
{"id": "PMID:462479", "title": "Effects of verapamil on the contractile force in the glycerinated dog heart muscles.", "content": "The effect of Ca-antagonist on the contractile apparatus was investigated in glycerinated cardiac papillary muscle preparations obtained from canine hearts. The results showed that maximal developed tension (P0) was enhanced significantly by 5 mg/liter of verapamil, and the augmentation of contractility was dependent on the concentrations of verapamil. As a conclusion, Ca-antagonist appeared to be a potentiating agent of the contractile force on the contractile apparatus.", "contents": "Effects of verapamil on the contractile force in the glycerinated dog heart muscles. The effect of Ca-antagonist on the contractile apparatus was investigated in glycerinated cardiac papillary muscle preparations obtained from canine hearts. The results showed that maximal developed tension (P0) was enhanced significantly by 5 mg/liter of verapamil, and the augmentation of contractility was dependent on the concentrations of verapamil. As a conclusion, Ca-antagonist appeared to be a potentiating agent of the contractile force on the contractile apparatus."}
{"id": "PMID:462481", "title": "[Comparison between conventional X-ray diagnostics and computed tomography as techniques for irradiation planning in renal carcinoma and carcinoma of the bladder (author's transl)].", "content": "In order to obtain quantitative results by means of a comparison between irradiation planning with X-ray diagnostics and planning with cross-sectional computed tomography in abdominal tumors, therapeutic programs have been established twice for the same patients, each time using one of the two techniques. Fifteen patients with renal carcinoma and 24 patients with cancer of the urinary bladder have been examined.", "contents": "[Comparison between conventional X-ray diagnostics and computed tomography as techniques for irradiation planning in renal carcinoma and carcinoma of the bladder (author's transl)]. In order to obtain quantitative results by means of a comparison between irradiation planning with X-ray diagnostics and planning with cross-sectional computed tomography in abdominal tumors, therapeutic programs have been established twice for the same patients, each time using one of the two techniques. Fifteen patients with renal carcinoma and 24 patients with cancer of the urinary bladder have been examined."}
{"id": "PMID:462482", "title": "[Late results after preirradiation of mastocarcinomas (author's transl)].", "content": "The results of 80 cases treated with preirradiations are described. The follow-up observation time was longer than ten years. The results are statistically evaluated. It must be emphasized that lenticular skin metastases could be prevented in most of the cases. Eleven cases of double mastocarcinoma were submitted to follow-up examinations. Only two of the patients whose first and second tumors were preirradiated showed a survival time of twelve to fourteen years.", "contents": "[Late results after preirradiation of mastocarcinomas (author's transl)]. The results of 80 cases treated with preirradiations are described. The follow-up observation time was longer than ten years. The results are statistically evaluated. It must be emphasized that lenticular skin metastases could be prevented in most of the cases. Eleven cases of double mastocarcinoma were submitted to follow-up examinations. Only two of the patients whose first and second tumors were preirradiated showed a survival time of twelve to fourteen years."}
{"id": "PMID:462483", "title": "Radiation-induced changes in cell proliferation kinetics in uterine cervix cancer.", "content": "In vitro double labeling autoradiography for measuring the labeling index, duration of S phase and potential doubling time has been used to assess the effect of a single and fractionated test dose of irradiation in uterine cervix cancer applied in 21 patients. Tumor-labeling index fell significantly in those tumors which had a high labeling index before irradiation, and these cases were found later to exhibit a good radiation response. Duration of S phase which ranged between 9 and 27 hours prior to irradiation was increased. Differences between the potential doubling time and the actual doubling time suggest a massive cell loss in tumors which were, for the most part, of exophytic type. Fractionated irradiation provides more reliable informations than a single dose and is useful for investigation of dynamic changes in the kinetics of an asynchronous cell population.", "contents": "Radiation-induced changes in cell proliferation kinetics in uterine cervix cancer. In vitro double labeling autoradiography for measuring the labeling index, duration of S phase and potential doubling time has been used to assess the effect of a single and fractionated test dose of irradiation in uterine cervix cancer applied in 21 patients. Tumor-labeling index fell significantly in those tumors which had a high labeling index before irradiation, and these cases were found later to exhibit a good radiation response. Duration of S phase which ranged between 9 and 27 hours prior to irradiation was increased. Differences between the potential doubling time and the actual doubling time suggest a massive cell loss in tumors which were, for the most part, of exophytic type. Fractionated irradiation provides more reliable informations than a single dose and is useful for investigation of dynamic changes in the kinetics of an asynchronous cell population."}
{"id": "PMID:462484", "title": "Anatomo-radiological study of a case of post-radiotherapeutic osteolysis of the hip.", "content": "Anatomo-radiological study of a case of osteolysis of the femoral head and neck in a 56-year old male who had recieved, 22 years earlier, an intense radiotherapeutic treatment for a soft tissue tumour of the corresponding hip region. The treatment is considered as being the main etiological factor responsible for the bone lesions. The latter seems to have been favored by an indolent joint due to a thoracic cordotomy for post-irradiation pains; it may have been prepared by a secondary osteodystrophy which preceded the radiotherapy and seems due to two previous surgical excisions.", "contents": "Anatomo-radiological study of a case of post-radiotherapeutic osteolysis of the hip. Anatomo-radiological study of a case of osteolysis of the femoral head and neck in a 56-year old male who had recieved, 22 years earlier, an intense radiotherapeutic treatment for a soft tissue tumour of the corresponding hip region. The treatment is considered as being the main etiological factor responsible for the bone lesions. The latter seems to have been favored by an indolent joint due to a thoracic cordotomy for post-irradiation pains; it may have been prepared by a secondary osteodystrophy which preceded the radiotherapy and seems due to two previous surgical excisions."}
{"id": "PMID:462485", "title": "[Three dimensional optimization method for the interstitional afterloading therapy (author's transl)].", "content": "The authors present a new method for the interstitial therapy working with an Ir-192 source which is displaced step by step. After having been fed with the tumor parameters, the computer calculates for every stopping point the duration of stay of the source which provides a threedimensional optimization. The practical application of the method is discussed and the optimization procedure is compared with other similar methods.", "contents": "[Three dimensional optimization method for the interstitional afterloading therapy (author's transl)]. The authors present a new method for the interstitial therapy working with an Ir-192 source which is displaced step by step. After having been fed with the tumor parameters, the computer calculates for every stopping point the duration of stay of the source which provides a threedimensional optimization. The practical application of the method is discussed and the optimization procedure is compared with other similar methods."}
{"id": "PMID:462486", "title": "[The small Esssen high-pressure oxygen chamber for experimental animals (author's transl)].", "content": "A small translucent high-pressure chamber is introduced which has been exploited for local irradiation of tumour-bearing mice. The chamber has particularly proven to be valuable for high-pressure oxygen therapy together with simultaneous irradiation but can be used also for normal pressure and for gas other than oxygen. The chamber is shown during operation and a diagram illustrates design and technical data.", "contents": "[The small Esssen high-pressure oxygen chamber for experimental animals (author's transl)]. A small translucent high-pressure chamber is introduced which has been exploited for local irradiation of tumour-bearing mice. The chamber has particularly proven to be valuable for high-pressure oxygen therapy together with simultaneous irradiation but can be used also for normal pressure and for gas other than oxygen. The chamber is shown during operation and a diagram illustrates design and technical data."}
{"id": "PMID:462487", "title": "[An improved method to stimulate water and biological tissues in relation to linear interactions with photons and fast electrons (author's transl)].", "content": "Radiation physical problems in clinical dosimetry often need for their experimental solution the linear coefficients of interaction between radiation and irradited matter. Using modified material parameters (the products of mass density and the earlier defined effective material parameters) it was possible to derive an improved method to calculate the composition of an equivalent for a material to be substituted. The method is described and its usefulness demonstrated by some examples. A geometric analogue facilitates the solution if a computer is not available. Some comparisons are made between this method and the basic data method of White.", "contents": "[An improved method to stimulate water and biological tissues in relation to linear interactions with photons and fast electrons (author's transl)]. Radiation physical problems in clinical dosimetry often need for their experimental solution the linear coefficients of interaction between radiation and irradited matter. Using modified material parameters (the products of mass density and the earlier defined effective material parameters) it was possible to derive an improved method to calculate the composition of an equivalent for a material to be substituted. The method is described and its usefulness demonstrated by some examples. A geometric analogue facilitates the solution if a computer is not available. Some comparisons are made between this method and the basic data method of White."}
{"id": "PMID:462489", "title": "Influence of whole body irradiation on intraperitoneal immunity in a syngeneic mouse lymphoma system.", "content": "DBA/2 mice were immunized against the syngeneic SL2 lymphoma by two injections with irradiated lymphoma cells or by two injections with irradiated cells followed by one injection with unirradiated cells. Whole body irradiation (500 rad) of the immunized mice caused a significant decrease in the number of peritoneal macrophages and lymphocytes. The spleen weight showed a transient decrease. However, whole body irradiation did not cause a significant decrease of the survival time of immunized mice challenged intraperitoneally with SL2 lymphoma cells.", "contents": "Influence of whole body irradiation on intraperitoneal immunity in a syngeneic mouse lymphoma system. DBA/2 mice were immunized against the syngeneic SL2 lymphoma by two injections with irradiated lymphoma cells or by two injections with irradiated cells followed by one injection with unirradiated cells. Whole body irradiation (500 rad) of the immunized mice caused a significant decrease in the number of peritoneal macrophages and lymphocytes. The spleen weight showed a transient decrease. However, whole body irradiation did not cause a significant decrease of the survival time of immunized mice challenged intraperitoneally with SL2 lymphoma cells."}
{"id": "PMID:462490", "title": "Heterocyclic steroids-part VIII: Studies on the total synthesis of racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol.", "content": "2-Phenyl-6-methyl-4-oxo-4,5,6,7-tetrahydrobenzofuran (II) is converted into racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol (VIII).", "contents": "Heterocyclic steroids-part VIII: Studies on the total synthesis of racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol. 2-Phenyl-6-methyl-4-oxo-4,5,6,7-tetrahydrobenzofuran (II) is converted into racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol (VIII)."}
{"id": "PMID:462491", "title": "Bile acid sulfates. III. Synthesis of 7- and 12-monosulfates of bile acids and their conjugates using a sulfur trioxide-triethylamine complex.", "content": "The 7- and 12-monosulfates of chenodeoxycholic acid, deoxycholic acid, and cholic acid were prepared by sulfation of the protected bile acids with sulfur trioxide-triethylamine in pyridine overnight and were isolated by precipitation as the p-toluidinium salt after removing the protecting group(s). The taurine conjugates were obtained by conjugating the bile acid sulfates with taurine in hot dimethylformamide (DMF) in the presence of N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ). A new procedure of preparing glycine conjugated bile acid sulfates by direct conjugation of the bile acid sulfate triethylammonium salt with ethyl glycinate in boiling chloroform in the presence of EEDQ is also described. The advantage of these procedures over other procedures are their simplicity and their higher yields (tyically above 90%) The thin layer chromatographic mobilities of these sulfates are presented. The influence of side chain and hydroxyl group configurations on the properties of bile acid sulfates is briefly discussed.", "contents": "Bile acid sulfates. III. Synthesis of 7- and 12-monosulfates of bile acids and their conjugates using a sulfur trioxide-triethylamine complex. The 7- and 12-monosulfates of chenodeoxycholic acid, deoxycholic acid, and cholic acid were prepared by sulfation of the protected bile acids with sulfur trioxide-triethylamine in pyridine overnight and were isolated by precipitation as the p-toluidinium salt after removing the protecting group(s). The taurine conjugates were obtained by conjugating the bile acid sulfates with taurine in hot dimethylformamide (DMF) in the presence of N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ). A new procedure of preparing glycine conjugated bile acid sulfates by direct conjugation of the bile acid sulfate triethylammonium salt with ethyl glycinate in boiling chloroform in the presence of EEDQ is also described. The advantage of these procedures over other procedures are their simplicity and their higher yields (tyically above 90%) The thin layer chromatographic mobilities of these sulfates are presented. The influence of side chain and hydroxyl group configurations on the properties of bile acid sulfates is briefly discussed."}
{"id": "PMID:462492", "title": "Serum levels of corticosterone and 18-hydroxy-11-deoxycorticosterone in the female rat at the high and low points of the circadian rhythm.", "content": "Serum corticosterone (B) and 18-hydroxy-11-deoxycorticosterone (18-hydroxy-DOC) levels were determined in female rats at the high (1800 h) and low (0600 h) points of the circadian rhythm. In order to carry out these studies, a rapid and accurate non-chromatographic radioimmunoassay method was developed for the measurement of 18-hydroxy-DOC in peripheral blood and similar methodology was used for the B assay. In quiescent rats both steroids were dramatically elevated at 1800 h as compared to 0600 h. The serum levels of B and 18-hydroxy-DOC determined at 1800 h fifteen minutes following stress did not differ significantly from the levels determined following a similar stress at 0600 h. There was a good correlation (r = 0.91) between the levels of B and 18-hydroxy-DOC and it appears that both steroids are regulated by ACTH.", "contents": "Serum levels of corticosterone and 18-hydroxy-11-deoxycorticosterone in the female rat at the high and low points of the circadian rhythm. Serum corticosterone (B) and 18-hydroxy-11-deoxycorticosterone (18-hydroxy-DOC) levels were determined in female rats at the high (1800 h) and low (0600 h) points of the circadian rhythm. In order to carry out these studies, a rapid and accurate non-chromatographic radioimmunoassay method was developed for the measurement of 18-hydroxy-DOC in peripheral blood and similar methodology was used for the B assay. In quiescent rats both steroids were dramatically elevated at 1800 h as compared to 0600 h. The serum levels of B and 18-hydroxy-DOC determined at 1800 h fifteen minutes following stress did not differ significantly from the levels determined following a similar stress at 0600 h. There was a good correlation (r = 0.91) between the levels of B and 18-hydroxy-DOC and it appears that both steroids are regulated by ACTH."}
{"id": "PMID:462493", "title": "In vitro metabolism of 4-androstene-3,17-dione and testosterone by rat submaxillary gland.", "content": "Tritiated 4-androstene-3,17-dione and testosterone were incubated with submaxillary gland homogenates of male and female rats. The metabolism was predominately reductive. In 15 and 180 min incubations submaxillary tissue converted 4-androstene-3,17-dione chiefly to androsterone. Less testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one, 5 alpha-androstane-3,17-dione, 5 alpha-androstane-3 alpha, 17 beta-diol, and 4-androstene-3 alpha, 17 beta-diol were also identified. Testosterone was converted to the same products plus 4-androstene-3,17-dione. 5 alpha-Androstane-3 alpha, 17 beta-diol was the major testosterone metabolite. Qualitatively the metabolism by male and female submaxillary gland was similar.", "contents": "In vitro metabolism of 4-androstene-3,17-dione and testosterone by rat submaxillary gland. Tritiated 4-androstene-3,17-dione and testosterone were incubated with submaxillary gland homogenates of male and female rats. The metabolism was predominately reductive. In 15 and 180 min incubations submaxillary tissue converted 4-androstene-3,17-dione chiefly to androsterone. Less testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one, 5 alpha-androstane-3,17-dione, 5 alpha-androstane-3 alpha, 17 beta-diol, and 4-androstene-3 alpha, 17 beta-diol were also identified. Testosterone was converted to the same products plus 4-androstene-3,17-dione. 5 alpha-Androstane-3 alpha, 17 beta-diol was the major testosterone metabolite. Qualitatively the metabolism by male and female submaxillary gland was similar."}
{"id": "PMID:462494", "title": "Photohydration of testosterone and 4-androstene-3,17-dione in aqueous solution.", "content": "Irradiation of testosterone, 4-androstene-3,17-dione, or their \"half-molecule\" 4,4a,5,6,7,8-hexahydro-4a-methyl-2(3H)-naphthalenone in dilute aqueous solutions with ultraviolet light of 254 nm wavelength caused rapid addition of water across the olefinic bond with formation of 5,17 beta-dihydroxy-5 alpha-androstan-3-one, 5-hydroxy-5 alpha-androstane-3-17-dione, and 9-hydroxy-10-methyl-2-decalone, respectively. Time-lapse spectrometry in the ultraviolet region showed that the photohydration of the androgenic steroids was extremely efficient and virtually free of the side reactions. Preparative photolytic reactions carried out in water-methanol solutions allowed isolation and characterization of photoproducts.", "contents": "Photohydration of testosterone and 4-androstene-3,17-dione in aqueous solution. Irradiation of testosterone, 4-androstene-3,17-dione, or their \"half-molecule\" 4,4a,5,6,7,8-hexahydro-4a-methyl-2(3H)-naphthalenone in dilute aqueous solutions with ultraviolet light of 254 nm wavelength caused rapid addition of water across the olefinic bond with formation of 5,17 beta-dihydroxy-5 alpha-androstan-3-one, 5-hydroxy-5 alpha-androstane-3-17-dione, and 9-hydroxy-10-methyl-2-decalone, respectively. Time-lapse spectrometry in the ultraviolet region showed that the photohydration of the androgenic steroids was extremely efficient and virtually free of the side reactions. Preparative photolytic reactions carried out in water-methanol solutions allowed isolation and characterization of photoproducts."}
{"id": "PMID:462495", "title": "Simultaneous radioimmunoassay of serum testosterone and 5 alpha-dihydrotestosterone without chromatography.", "content": "When characterization of the specificity of an antiserum for radioimmunoassay (RIA) is performed by the conventional method, the conditions under which interference occurs are not respected because of the lack of specific antigen. We have studied the behavior of antisera reproducing the real environment existing in unknown samples, in which antigen, interferent and tracer complete simultaneously. A testosterone (T) antiserum and a 5 alpha-dihydrotestosterone (D) anti serum were characterized by setting up two distinct hapten recovery tests in the presence of both the hapten and the crossreactant added to steroid-free serum in various concentrations in order to reproduce multiple concentration ratios. These samples, together with the standard curves samples (prepared by 'spiking' steroid-free serum with known concentrations of T or D) were extracted and subjected to T-RIA and D-RIA without purification. The results have shown that the interferent-induced incremental ratio is a linear function of the ratio of the levels of cross-reactant and hapten via a proportionality factor inversely correlated to the antiserum specificity. By means of this function, the overestimated T and D levels found in samples after 'extraction only' have been corrected and the resulting values have shown acceptable correlation with the corresponding levels determined after column chromatography.", "contents": "Simultaneous radioimmunoassay of serum testosterone and 5 alpha-dihydrotestosterone without chromatography. When characterization of the specificity of an antiserum for radioimmunoassay (RIA) is performed by the conventional method, the conditions under which interference occurs are not respected because of the lack of specific antigen. We have studied the behavior of antisera reproducing the real environment existing in unknown samples, in which antigen, interferent and tracer complete simultaneously. A testosterone (T) antiserum and a 5 alpha-dihydrotestosterone (D) anti serum were characterized by setting up two distinct hapten recovery tests in the presence of both the hapten and the crossreactant added to steroid-free serum in various concentrations in order to reproduce multiple concentration ratios. These samples, together with the standard curves samples (prepared by 'spiking' steroid-free serum with known concentrations of T or D) were extracted and subjected to T-RIA and D-RIA without purification. The results have shown that the interferent-induced incremental ratio is a linear function of the ratio of the levels of cross-reactant and hapten via a proportionality factor inversely correlated to the antiserum specificity. By means of this function, the overestimated T and D levels found in samples after 'extraction only' have been corrected and the resulting values have shown acceptable correlation with the corresponding levels determined after column chromatography."}
{"id": "PMID:462496", "title": "A novel fluorinated derivative of diethylstilbestrol.", "content": "The synthesis of the diethylstilbestrol (DES) derivative with fluorine atoms present in the positions ortho to the hydroxyl in each ring is described. In vitro studies in a system containing horse radish peroxidase/H2O2 demonstrate extensive oxidation of tetrafluorodiethylstilbestrol to the corresponding dienestrol derivative. Tetrafluorodiethylstilbestrol and DES had comparable in vivo uterotropic activities at a dose of 100 microgram/kg. Competitive binding experiments demonstrated 20-25 fold reduced interaction with the mouse uterine estrogen receptor. This compound may be useful as an experimental estrogen in distinguishing between the biological and toxic effects of DES.", "contents": "A novel fluorinated derivative of diethylstilbestrol. The synthesis of the diethylstilbestrol (DES) derivative with fluorine atoms present in the positions ortho to the hydroxyl in each ring is described. In vitro studies in a system containing horse radish peroxidase/H2O2 demonstrate extensive oxidation of tetrafluorodiethylstilbestrol to the corresponding dienestrol derivative. Tetrafluorodiethylstilbestrol and DES had comparable in vivo uterotropic activities at a dose of 100 microgram/kg. Competitive binding experiments demonstrated 20-25 fold reduced interaction with the mouse uterine estrogen receptor. This compound may be useful as an experimental estrogen in distinguishing between the biological and toxic effects of DES."}
{"id": "PMID:462497", "title": "Radioimmunoassay of 2-hydroxyesterone and 2-methoxyestrone in human urine.", "content": "An assay for the quantitative determination of 2-hydroxyestrone and 2-methoxyestrone in human urine is described. The analytical procedure involves several purification steps: XAD-2 column chromatography of urine (1 ml), hot acid hydrolysis under reducing conditions, extraction with benzene/ethyl acetate, separation of monophenolic steroids from catecholestrogens by the formation of borate complexes, and partition between different solvents. Quantitation is achieved by radioimmunoassay using highly specific antibodies. For correction of procedural losses [6, 7-3H2] 2-hydroxyestrone and [6, 7-3H2] 2-methoxyestrone are used as internal standards. The method is highly specific as checked by comparison to a double-isotope-derivative method and a newly developed gas chromatography-mass spectrometry procedure. Using this method the urinary excretion of 2-hydroxyestrone and 2-methoxyestrone was studied in children, men, cycling, pregnant and postmenopausal women. Special interest was focussed on the molar ratios of 2-hydroxyestrone to 2-methoxyestrone and the so called \"total estrogens\" which vary markedly within the different groups investigated. The excretion of 2-hydroxyestrone is especially favoured in women during the menstrual cycle and pregnancy.", "contents": "Radioimmunoassay of 2-hydroxyesterone and 2-methoxyestrone in human urine. An assay for the quantitative determination of 2-hydroxyestrone and 2-methoxyestrone in human urine is described. The analytical procedure involves several purification steps: XAD-2 column chromatography of urine (1 ml), hot acid hydrolysis under reducing conditions, extraction with benzene/ethyl acetate, separation of monophenolic steroids from catecholestrogens by the formation of borate complexes, and partition between different solvents. Quantitation is achieved by radioimmunoassay using highly specific antibodies. For correction of procedural losses [6, 7-3H2] 2-hydroxyestrone and [6, 7-3H2] 2-methoxyestrone are used as internal standards. The method is highly specific as checked by comparison to a double-isotope-derivative method and a newly developed gas chromatography-mass spectrometry procedure. Using this method the urinary excretion of 2-hydroxyestrone and 2-methoxyestrone was studied in children, men, cycling, pregnant and postmenopausal women. Special interest was focussed on the molar ratios of 2-hydroxyestrone to 2-methoxyestrone and the so called \"total estrogens\" which vary markedly within the different groups investigated. The excretion of 2-hydroxyestrone is especially favoured in women during the menstrual cycle and pregnancy."}
{"id": "PMID:462498", "title": "Metabolism of progesterone in the pregnant sheep near term: identification of 3 beta-hydroxy-5 alpha-pregnan-20-one 3-sulfate as a major metabolite.", "content": "Two pregnant ewes near term were given a single injection of progesterone-4-14C via the left jugular vein, and serial blood samples were taken from the right jugular vein at 5 min intervals over a period of 40 min. Radioactive steroids in the plasma were separated into unconjugated and conjugated fractions which were further isolated and analysed by established methods. The injected hormone was rapidly metabolized with a half-life of approximately 10 min and metabolic clearance rate about 3.5 liters min. The bulk of the metabolites was found in the sulfate fraction from which a major metabolite was identified as 3 beta-hydroxy-5 alpha-pregnan-20-one. From the unconjugated fraction, 20 alpha-hydroxy-pregn-4-en-3-one, a known minor metabolite was also isolated. No radioactive estrogens were found. It is concluded that a major portion of circulating progesterone in the pregnant ewe near term is cleared by 5 alpha-reduction of ring A, followed by sulfo-conjugation.", "contents": "Metabolism of progesterone in the pregnant sheep near term: identification of 3 beta-hydroxy-5 alpha-pregnan-20-one 3-sulfate as a major metabolite. Two pregnant ewes near term were given a single injection of progesterone-4-14C via the left jugular vein, and serial blood samples were taken from the right jugular vein at 5 min intervals over a period of 40 min. Radioactive steroids in the plasma were separated into unconjugated and conjugated fractions which were further isolated and analysed by established methods. The injected hormone was rapidly metabolized with a half-life of approximately 10 min and metabolic clearance rate about 3.5 liters min. The bulk of the metabolites was found in the sulfate fraction from which a major metabolite was identified as 3 beta-hydroxy-5 alpha-pregnan-20-one. From the unconjugated fraction, 20 alpha-hydroxy-pregn-4-en-3-one, a known minor metabolite was also isolated. No radioactive estrogens were found. It is concluded that a major portion of circulating progesterone in the pregnant ewe near term is cleared by 5 alpha-reduction of ring A, followed by sulfo-conjugation."}
{"id": "PMID:462499", "title": "Improved synthesis of 5 beta-cholestan-26-oic acids.", "content": "An improved method for the synthesis of 3 alpha, 7 alpha-dihydroxy-5 beta-cholestan-26-oic acid and 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid is described. The method involves an Arndt-Eistert rearrangement of the corresponding diazoketone obtained by the action of diazoethane on 3 alpha, 7 alpha-diformyloxy-5 beta-cholane-24-carboxylic or 3 alpha, 7 alpha, 12 alpha-triformyloxy-5 beta-cholane-24-carboxylic acid chloride. The products are obtained in good yield and no isomeric 27-nor- 24-methyl acid contaminants are formed as encountered in the commonly employed Kolbe synthesis.", "contents": "Improved synthesis of 5 beta-cholestan-26-oic acids. An improved method for the synthesis of 3 alpha, 7 alpha-dihydroxy-5 beta-cholestan-26-oic acid and 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid is described. The method involves an Arndt-Eistert rearrangement of the corresponding diazoketone obtained by the action of diazoethane on 3 alpha, 7 alpha-diformyloxy-5 beta-cholane-24-carboxylic or 3 alpha, 7 alpha, 12 alpha-triformyloxy-5 beta-cholane-24-carboxylic acid chloride. The products are obtained in good yield and no isomeric 27-nor- 24-methyl acid contaminants are formed as encountered in the commonly employed Kolbe synthesis."}
{"id": "PMID:462500", "title": "Determination of the configuration of epimeric diols of vitamin D3 series by mass spectrometry.", "content": "The mass spectral elimination of water in epimeric 1,3-diols of vitamin D3 (colecalciferol) series has been investigated. It was found that the mass spectra of these steroisomers differ sharply in the relative intensities of the ions M-H2O (m/e 382) and a-H2O (m/e +/- 34), where ion a (formed via formal cleavage of the 7, 8-double bond) is characteristic of vitamin D3 and its derivatives. So while epimeric 1, 3-diols of vitamin D3 series have very close UV and NMR characteristics, the comparison of the ratios of the peaks M-H2O and M.+, a-H2O and a, respectively, makes it possible to distinguish between stereoisomeric 1 alpha, 3 beta-, 1 beta, 3 beta-, 1 alpha, 3 alpha- and 1 beta, 3 alpha-diols using their mass spectra.", "contents": "Determination of the configuration of epimeric diols of vitamin D3 series by mass spectrometry. The mass spectral elimination of water in epimeric 1,3-diols of vitamin D3 (colecalciferol) series has been investigated. It was found that the mass spectra of these steroisomers differ sharply in the relative intensities of the ions M-H2O (m/e 382) and a-H2O (m/e +/- 34), where ion a (formed via formal cleavage of the 7, 8-double bond) is characteristic of vitamin D3 and its derivatives. So while epimeric 1, 3-diols of vitamin D3 series have very close UV and NMR characteristics, the comparison of the ratios of the peaks M-H2O and M.+, a-H2O and a, respectively, makes it possible to distinguish between stereoisomeric 1 alpha, 3 beta-, 1 beta, 3 beta-, 1 alpha, 3 alpha- and 1 beta, 3 alpha-diols using their mass spectra."}
{"id": "PMID:462502", "title": "Synthesis of C-19 deuterium labelled steroids.", "content": "Preparation of the synthetically useful steroid intermediate 19-d3-androst-4-ene-3,17-dione (Ia) together with its 19-d2-(Ib) and 19-d1-(Ic) analogues is described. The conditions and work-up of the synthesis have been designed to eliminate tedious chromatographic separation and purification steps thus enabling decigrams of material to be conveniently prepared using standard laboratory apparatus. The deuterium label in the C-19 angular methyl group is inert to normal chemical exchange processes, thus offering the opportunity for synthesis of more complex, biologically active, stable labelled steroids, whose metabolism can be studied mass spectrometrically.", "contents": "Synthesis of C-19 deuterium labelled steroids. Preparation of the synthetically useful steroid intermediate 19-d3-androst-4-ene-3,17-dione (Ia) together with its 19-d2-(Ib) and 19-d1-(Ic) analogues is described. The conditions and work-up of the synthesis have been designed to eliminate tedious chromatographic separation and purification steps thus enabling decigrams of material to be conveniently prepared using standard laboratory apparatus. The deuterium label in the C-19 angular methyl group is inert to normal chemical exchange processes, thus offering the opportunity for synthesis of more complex, biologically active, stable labelled steroids, whose metabolism can be studied mass spectrometrically."}
{"id": "PMID:462503", "title": "The source of progesterone in preimplantation rabbit blastocysts.", "content": "In vitro and in vivo synthesis of progesterone, sequestration of progesterone from the surrounding medium, and its subsequent conversion to metabolites was investigated in 146 hr post coitus preimplantation rabbit blastocysts. No significant conversion of 3H-pregnenolone to 3H-progesterone was observed throughout the 8 hr incubation. Progesterone content in blastocysts and culture medium did not change during the course of an 8 hr incubation. This suggests that the failure to detect incorporation of label into progesterone was not due to the presence of a large endogenous pool of pregnenolone. Significant uptake (p less than 0.05) of 3H-progesterone from the incubation medium was observed as was significant conversion of the 3H-progesterone to unidentified metabolites. Therefore it would appear that the preimplantation rabbit blastocyst is not capable of de novo synthesis of progesterone from pregnenolone prior to implantation but sequesters progesterone from the surrounding medium and converts it to progesterone metabolites.", "contents": "The source of progesterone in preimplantation rabbit blastocysts. In vitro and in vivo synthesis of progesterone, sequestration of progesterone from the surrounding medium, and its subsequent conversion to metabolites was investigated in 146 hr post coitus preimplantation rabbit blastocysts. No significant conversion of 3H-pregnenolone to 3H-progesterone was observed throughout the 8 hr incubation. Progesterone content in blastocysts and culture medium did not change during the course of an 8 hr incubation. This suggests that the failure to detect incorporation of label into progesterone was not due to the presence of a large endogenous pool of pregnenolone. Significant uptake (p less than 0.05) of 3H-progesterone from the incubation medium was observed as was significant conversion of the 3H-progesterone to unidentified metabolites. Therefore it would appear that the preimplantation rabbit blastocyst is not capable of de novo synthesis of progesterone from pregnenolone prior to implantation but sequesters progesterone from the surrounding medium and converts it to progesterone metabolites."}
{"id": "PMID:462504", "title": "The identification and characterization of a new C19O3 steroid metabolite in the rat ventral prostate: 5 alpha-androstane-3 beta, 6 alpha, 17 beta-triol.", "content": "This study represents the first report of the formation of 5 alpha-androstane-3 beta, 6 alpha, 17 beta-triol (6 alpha-triol) by prostatic tissue. The 6 alpha-triol has been identified by rigorous methods and a chemical synthesis of this triol has been accomplished. This 6 alpha-triol is the major metabolite of 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) in the rat ventral prostate. A minor metabolite of 3 beta-diol has been identified as 5 alpha-androstane-3 beta, 7 alpha, 17 beta-triol (7 alpha-triol). Using a variety of C19 androstane substrates, the 6 alpha- and 7 alpha-triols were always found as the major components of the total 3 beta-hydroxy-5 alpha-androstane metabolites produced by the ventral prostate. Following intraperitoneal injection of 3H-3 beta-diol, both 6 alpha- and 7 alpha-triol were formed in vivo by the ventral prostate and found in the blood. The 6 alpha- and 7 alpha-triols were found to possess no androgenic activity when tested by the ventral prostatic growth bioassay in the castrate rat.", "contents": "The identification and characterization of a new C19O3 steroid metabolite in the rat ventral prostate: 5 alpha-androstane-3 beta, 6 alpha, 17 beta-triol. This study represents the first report of the formation of 5 alpha-androstane-3 beta, 6 alpha, 17 beta-triol (6 alpha-triol) by prostatic tissue. The 6 alpha-triol has been identified by rigorous methods and a chemical synthesis of this triol has been accomplished. This 6 alpha-triol is the major metabolite of 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) in the rat ventral prostate. A minor metabolite of 3 beta-diol has been identified as 5 alpha-androstane-3 beta, 7 alpha, 17 beta-triol (7 alpha-triol). Using a variety of C19 androstane substrates, the 6 alpha- and 7 alpha-triols were always found as the major components of the total 3 beta-hydroxy-5 alpha-androstane metabolites produced by the ventral prostate. Following intraperitoneal injection of 3H-3 beta-diol, both 6 alpha- and 7 alpha-triol were formed in vivo by the ventral prostate and found in the blood. The 6 alpha- and 7 alpha-triols were found to possess no androgenic activity when tested by the ventral prostatic growth bioassay in the castrate rat."}
{"id": "PMID:462505", "title": "Reversible interaction between androgen binding protein and testicular macromolecules causing inhibition of androgen binding activity.", "content": "Sertoli cells in culture isolated from immature rat testes secrete androgen binding protein (ABP) in the culture medium. Binding activity of ABP in concentrated medium was estimated with equilibrium dialysis against 1 nM dihydrotestosterone at 4 degrees C. The ABP protein activity was inhibited approximately 50% through addition of cytosol preparations from testis or liver, but not from brain tissue, to the concentrated culture medium; this inhibition remained constant for at least two days. The inhibitor is probably a macromolecule, because the activity could not be removed by charcoal treatment and dialysis. The percent inhibition of ABP binding activity was increased when increasing amounts of cytosol were added, it decreased in the presence of increased concentrations of androgens, but it was not influenced by variations of the concentration of ABP. Inhibition of androgen binding to ABP by cytosols in the presence of 1 nM testosterone could be reversed after dialysis in the presence of 10 nM testosterone. These results suggest a reversible competition between testosterone and the testicular macromolecule for ABP. The occurrence of this interaction between ABP and a testicular macromolecule can explain the variable results of estimated ABP binding activity in testis cytosol preparations.", "contents": "Reversible interaction between androgen binding protein and testicular macromolecules causing inhibition of androgen binding activity. Sertoli cells in culture isolated from immature rat testes secrete androgen binding protein (ABP) in the culture medium. Binding activity of ABP in concentrated medium was estimated with equilibrium dialysis against 1 nM dihydrotestosterone at 4 degrees C. The ABP protein activity was inhibited approximately 50% through addition of cytosol preparations from testis or liver, but not from brain tissue, to the concentrated culture medium; this inhibition remained constant for at least two days. The inhibitor is probably a macromolecule, because the activity could not be removed by charcoal treatment and dialysis. The percent inhibition of ABP binding activity was increased when increasing amounts of cytosol were added, it decreased in the presence of increased concentrations of androgens, but it was not influenced by variations of the concentration of ABP. Inhibition of androgen binding to ABP by cytosols in the presence of 1 nM testosterone could be reversed after dialysis in the presence of 10 nM testosterone. These results suggest a reversible competition between testosterone and the testicular macromolecule for ABP. The occurrence of this interaction between ABP and a testicular macromolecule can explain the variable results of estimated ABP binding activity in testis cytosol preparations."}
{"id": "PMID:462508", "title": "The carotid bifurcation plaque: pathologic findings associated with cerebral ischemia.", "content": "Embolization from or decreased flow through cervical carotid and vertebral arteries causes ischemic stroke syndromes. Specific pathologic findings were studied in 50 symptomatic patients who underwent 69 carotid endarterectomies. Detailed analyses of their carotid plaques included correlations between photographs of gross specimens, microscopic findings, angiograms, preoperative symptoms and long-term postoperative follow up. Carotid plaques were primarily fibrous with significant (greater than 70%) stenoses encountered in 70% of the arteries. Stenoses were due to simple fibrous thickening in only 20%; the remainder due to intraplaque hemorrhage, atheromatous debris and, least often, luminal thrombus with or without ulceration. Intramural hemorrhage was frequent in plaques associated with focal neurologic symptoms and may have preceded localized collections of atheromatous debris. Ulceration occurred in 1/3 of all plaques, symptomatic or not. It is concluded that the carotid plaques start as fibrointimal thickening evolving to symptomatic stages by the occurrence of one or more of a number of pathologic changes, intraplaque hemorrhage being prominent. A single rational therapeutic regimen seems impossible until patients can be classified according to their pathologic changes diagnosed non-invasively.", "contents": "The carotid bifurcation plaque: pathologic findings associated with cerebral ischemia. Embolization from or decreased flow through cervical carotid and vertebral arteries causes ischemic stroke syndromes. Specific pathologic findings were studied in 50 symptomatic patients who underwent 69 carotid endarterectomies. Detailed analyses of their carotid plaques included correlations between photographs of gross specimens, microscopic findings, angiograms, preoperative symptoms and long-term postoperative follow up. Carotid plaques were primarily fibrous with significant (greater than 70%) stenoses encountered in 70% of the arteries. Stenoses were due to simple fibrous thickening in only 20%; the remainder due to intraplaque hemorrhage, atheromatous debris and, least often, luminal thrombus with or without ulceration. Intramural hemorrhage was frequent in plaques associated with focal neurologic symptoms and may have preceded localized collections of atheromatous debris. Ulceration occurred in 1/3 of all plaques, symptomatic or not. It is concluded that the carotid plaques start as fibrointimal thickening evolving to symptomatic stages by the occurrence of one or more of a number of pathologic changes, intraplaque hemorrhage being prominent. A single rational therapeutic regimen seems impossible until patients can be classified according to their pathologic changes diagnosed non-invasively."}
{"id": "PMID:462509", "title": "Scanning electron microscopic study of endothelial cells of cerebral arteries from spontaneously hypertensive rats.", "content": "Endothelial cells of the cerebral arterial system in spontaneously hypertensive rats were investigated by scanning electronmicroscopy and found to show progressive changes such as increased microvilli, numerous plasmalemmal pits, enlargement of the cells and well-developed marginal folds. Regressive changes, such as balloon-like protrusions and crater-like cave-ins, were also observed. Platelet adhesion to the injured endothelial surface of cerebral arteries was frequent. The significance of these changes in the development of hypertensive cerebrovascular lesions is discussed.", "contents": "Scanning electron microscopic study of endothelial cells of cerebral arteries from spontaneously hypertensive rats. Endothelial cells of the cerebral arterial system in spontaneously hypertensive rats were investigated by scanning electronmicroscopy and found to show progressive changes such as increased microvilli, numerous plasmalemmal pits, enlargement of the cells and well-developed marginal folds. Regressive changes, such as balloon-like protrusions and crater-like cave-ins, were also observed. Platelet adhesion to the injured endothelial surface of cerebral arteries was frequent. The significance of these changes in the development of hypertensive cerebrovascular lesions is discussed."}
{"id": "PMID:462510", "title": "The electrocardiogram in stroke: relationship to pathophysiological type and comparison with prior tracings.", "content": "The author reviewed electrocardiographic records of 150 patients with acute stroke and 150 age- and sex-matched controls, to assess the relative frequencies of ECG abnormalities among the pathophysiologic categories of stroke, and to distinguish new abnormalities at the time of the stroke from those noted on prior tracings. Of the 150 patients with stroke, 138 (92%) showed ECG abnormalities. The most common abnormalities were also changes from prior tracings: QT prolongation (68 patients, 45%), ischemic changes (59, 35%), U waves (42, 28%), tachycardia (42, 28%), and arrhythmias (41, 27%). Patients with cerebral embolus had a significantly increased frequency of atrial fibrillation (9 patients, 47%); and with subarachnoid hemorrhage an increased frequency of QT prolongation (20, 71%) and sinus arrhythmia (5, 18%). The frequencies of QT prolongation and ischemic changes related strongly to admission systolic pressure but not to mortality. Stroke patients had an increased frequency of pathologic Q waves (30 patients, 20%) and left ventricular hypertrophy (39, 26%), but these were not new findings at the time of the stroke. The results are consistent with an interaction of underlying hypertensive or atherosclerotic cardiovascular disease, sympathetic hyperactivity, and possibly myocardial necrosis, in producing ECG changes.", "contents": "The electrocardiogram in stroke: relationship to pathophysiological type and comparison with prior tracings. The author reviewed electrocardiographic records of 150 patients with acute stroke and 150 age- and sex-matched controls, to assess the relative frequencies of ECG abnormalities among the pathophysiologic categories of stroke, and to distinguish new abnormalities at the time of the stroke from those noted on prior tracings. Of the 150 patients with stroke, 138 (92%) showed ECG abnormalities. The most common abnormalities were also changes from prior tracings: QT prolongation (68 patients, 45%), ischemic changes (59, 35%), U waves (42, 28%), tachycardia (42, 28%), and arrhythmias (41, 27%). Patients with cerebral embolus had a significantly increased frequency of atrial fibrillation (9 patients, 47%); and with subarachnoid hemorrhage an increased frequency of QT prolongation (20, 71%) and sinus arrhythmia (5, 18%). The frequencies of QT prolongation and ischemic changes related strongly to admission systolic pressure but not to mortality. Stroke patients had an increased frequency of pathologic Q waves (30 patients, 20%) and left ventricular hypertrophy (39, 26%), but these were not new findings at the time of the stroke. The results are consistent with an interaction of underlying hypertensive or atherosclerotic cardiovascular disease, sympathetic hyperactivity, and possibly myocardial necrosis, in producing ECG changes."}
{"id": "PMID:462511", "title": "Transient ischemic attacks. Retrospective study of 150 cases of ischemic infarct in the territory of the middle cerebral artery.", "content": "Transient ischemic attacks (TIA) are episodes of abrupt beginning, consisting of subjective or objective neurological dysfunction of short duration, with complete recovery of neurological function in the course of 24 hours. With this definition, the authors carried out a retrospective study of 150 patients suffering from ischemic infarct in the brain in the territory of the middle cerebral artery. Thirty-eight percent of the patients had had TIAs before their cerebral infarct. The symptoms, in order of frequency, were motor, sensory deficits, alterations of speech and vision. Most of the patients had a definite cerebral infarct, occurring one month after the last TIA; the symptoms of both processes were remarkably similar. The authors studied the angiographic characteristics, pharmacological and toxic antecedents, and associated diseases in these patients. The study indicates that TIA may be the first manifestation of cerebral vascular disease.", "contents": "Transient ischemic attacks. Retrospective study of 150 cases of ischemic infarct in the territory of the middle cerebral artery. Transient ischemic attacks (TIA) are episodes of abrupt beginning, consisting of subjective or objective neurological dysfunction of short duration, with complete recovery of neurological function in the course of 24 hours. With this definition, the authors carried out a retrospective study of 150 patients suffering from ischemic infarct in the brain in the territory of the middle cerebral artery. Thirty-eight percent of the patients had had TIAs before their cerebral infarct. The symptoms, in order of frequency, were motor, sensory deficits, alterations of speech and vision. Most of the patients had a definite cerebral infarct, occurring one month after the last TIA; the symptoms of both processes were remarkably similar. The authors studied the angiographic characteristics, pharmacological and toxic antecedents, and associated diseases in these patients. The study indicates that TIA may be the first manifestation of cerebral vascular disease."}
{"id": "PMID:462512", "title": "Hypertension and acute focal cerebral ischemia. Infarction and edema after occlusion of a middle cerebral artery in cats.", "content": "Hypertension was produced in 8 cats by nephrectomy and wrapping the opposite kidney. Subsequent occlusion of one middle cerebral artery caused ischemic infarcts that were larger than those of 8 normotensive cats. The larger infarcts may have been caused by increases of ischemic cerebral edema resulting from changes in the cerebral endothelial barrier induced by hypertension. In addition to increasing the likelihood of strokes, hypertension in humans may predispose toward larger cerebral infarcts.", "contents": "Hypertension and acute focal cerebral ischemia. Infarction and edema after occlusion of a middle cerebral artery in cats. Hypertension was produced in 8 cats by nephrectomy and wrapping the opposite kidney. Subsequent occlusion of one middle cerebral artery caused ischemic infarcts that were larger than those of 8 normotensive cats. The larger infarcts may have been caused by increases of ischemic cerebral edema resulting from changes in the cerebral endothelial barrier induced by hypertension. In addition to increasing the likelihood of strokes, hypertension in humans may predispose toward larger cerebral infarcts."}
{"id": "PMID:462513", "title": "Occlusion of the vertebral or basilar artery. Follow up analysis of some patients with benign outcome.", "content": "Ten patients with angiographically verified occlusion of the basilar or vertebral artery have been followed for an average of 2.75 years. None has developed further ischemia after the initial stroke, and 4 patients survived without any clinical deficit. In occlusive disease of the posterior circulation, the critical period for deficit acquisition is at the time of occlusion. Extent of the deficit depends on the rapidity of development of adequate collateral circulation, and the presence of distal embolization at the time of occlusion. Some patients survive basilar occlusion without permanent deficit.", "contents": "Occlusion of the vertebral or basilar artery. Follow up analysis of some patients with benign outcome. Ten patients with angiographically verified occlusion of the basilar or vertebral artery have been followed for an average of 2.75 years. None has developed further ischemia after the initial stroke, and 4 patients survived without any clinical deficit. In occlusive disease of the posterior circulation, the critical period for deficit acquisition is at the time of occlusion. Extent of the deficit depends on the rapidity of development of adequate collateral circulation, and the presence of distal embolization at the time of occlusion. Some patients survive basilar occlusion without permanent deficit."}
{"id": "PMID:462514", "title": "Brain hemorrhage from intracranial tumor.", "content": "Brain hemorrhage from an intracranial tumor was encountered in 7 males and 6 females during a 4-year period. In 5 patients, hemorrhage was responsible for the first signs of a previously unsuspected neoplasm. The intracranial lesion was demonstrated by computed tomography (CT scanning) in each patient. Characteristic CT scan findings included: a neoplastic core (high or low density); small, multifocal clots usually at the margin of the tumor; and, surrounding, often extensive, edema. Enhancement of the tumor tissue with intravenous injection of 60% Hypaque was observed in the 8 patients so studied. The regions which were enhanced had a peripheral distribution corresponding to the site of hemorrhage. Microscopic examination demonstrated 7 glioblastoma multiforme, 1 oligodendroglioma, 4 metastatic carcinomas (including 1 each of bronchogenic carcinoma, melanoma, hypernephroma, and adrenal carcinoma), and 1 hemangiopericytoma. High-grade malignancy and extensive, abnormal vascularity appeared to be predisposing factors.", "contents": "Brain hemorrhage from intracranial tumor. Brain hemorrhage from an intracranial tumor was encountered in 7 males and 6 females during a 4-year period. In 5 patients, hemorrhage was responsible for the first signs of a previously unsuspected neoplasm. The intracranial lesion was demonstrated by computed tomography (CT scanning) in each patient. Characteristic CT scan findings included: a neoplastic core (high or low density); small, multifocal clots usually at the margin of the tumor; and, surrounding, often extensive, edema. Enhancement of the tumor tissue with intravenous injection of 60% Hypaque was observed in the 8 patients so studied. The regions which were enhanced had a peripheral distribution corresponding to the site of hemorrhage. Microscopic examination demonstrated 7 glioblastoma multiforme, 1 oligodendroglioma, 4 metastatic carcinomas (including 1 each of bronchogenic carcinoma, melanoma, hypernephroma, and adrenal carcinoma), and 1 hemangiopericytoma. High-grade malignancy and extensive, abnormal vascularity appeared to be predisposing factors."}
{"id": "PMID:462516", "title": "EEG monitoring for induced hypotension for surgery of intracranial aneurysms.", "content": "EEG was monitored at bilateral scalp sites outside the operative field during hypotensive aneurysm surgery in 21 patients. Mean arterial blood pressure at axillary level was 50-60 mm Hg (average 55 mm) for 1.9-5.3 hours (average 3.6). Four new deficits were noted immediately post-operatively, all related to the operated site: these were attributable to intra-operative rupture with forced vascular clipping, vasospasm, or edema. In no instance was hypotension solely responsible for a new deficit. EEG showed persistent slowing in relation to surgery in only 1 patient, where aneurysmal rupture led to severe hypotension, forced clipping of 1 posterior cerebral artery, and subsequent brain stem infarction. In the 3 other patients with fresh focal postoperative deficits, no persistent intraoperative EEG changes were observed. EEG monitoring did not detect ischemia in these 3 patients because 1) hypotension was moderate and did not per se cause new deficit, and 2) EEG electrodes did not survey the area at maximal risk, namely the operative field. EEG scalp electrodes near but outside the operative site do not seem useful for monitoring cerebral function in the region of aneurysm surgery. Epidural or cortical electrodes in the operative field may prove to be more useful.", "contents": "EEG monitoring for induced hypotension for surgery of intracranial aneurysms. EEG was monitored at bilateral scalp sites outside the operative field during hypotensive aneurysm surgery in 21 patients. Mean arterial blood pressure at axillary level was 50-60 mm Hg (average 55 mm) for 1.9-5.3 hours (average 3.6). Four new deficits were noted immediately post-operatively, all related to the operated site: these were attributable to intra-operative rupture with forced vascular clipping, vasospasm, or edema. In no instance was hypotension solely responsible for a new deficit. EEG showed persistent slowing in relation to surgery in only 1 patient, where aneurysmal rupture led to severe hypotension, forced clipping of 1 posterior cerebral artery, and subsequent brain stem infarction. In the 3 other patients with fresh focal postoperative deficits, no persistent intraoperative EEG changes were observed. EEG monitoring did not detect ischemia in these 3 patients because 1) hypotension was moderate and did not per se cause new deficit, and 2) EEG electrodes did not survey the area at maximal risk, namely the operative field. EEG scalp electrodes near but outside the operative site do not seem useful for monitoring cerebral function in the region of aneurysm surgery. Epidural or cortical electrodes in the operative field may prove to be more useful."}
{"id": "PMID:462517", "title": "Dissecting aneurysms of the basilar artery in 2 patients.", "content": "An uncommon consequence of intracranial vascular disease is the intramural dissection of blood or \"dissecting aneurysm\". A 69-year-old man with chronic subarachnoid hemorrhage from a posterior fossa mass lesion and a 30-year-old man with migraine and a brain stem stroke illustrate the diverse etiologic, clinical, radiographic, and pathologic characteristics of this unusual lesion.", "contents": "Dissecting aneurysms of the basilar artery in 2 patients. An uncommon consequence of intracranial vascular disease is the intramural dissection of blood or \"dissecting aneurysm\". A 69-year-old man with chronic subarachnoid hemorrhage from a posterior fossa mass lesion and a 30-year-old man with migraine and a brain stem stroke illustrate the diverse etiologic, clinical, radiographic, and pathologic characteristics of this unusual lesion."}
{"id": "PMID:462518", "title": "Isoproterenol treatment of visual symptoms in migraine.", "content": "Six patients with transient or permanent visual loss associated with migraine are presented. In 3 patients with monocular and one patient with binocular episodes of transient visual loss subsequent visual episodes were relieved by prompt inhalation of isoproterenol. The authors review the possible mechanisms of action of isoproterenol in migraine and present evidence to support the prophylactic use of isoproterenol to prevent transient and possible persistent visual loss in patients with migraine.", "contents": "Isoproterenol treatment of visual symptoms in migraine. Six patients with transient or permanent visual loss associated with migraine are presented. In 3 patients with monocular and one patient with binocular episodes of transient visual loss subsequent visual episodes were relieved by prompt inhalation of isoproterenol. The authors review the possible mechanisms of action of isoproterenol in migraine and present evidence to support the prophylactic use of isoproterenol to prevent transient and possible persistent visual loss in patients with migraine."}
{"id": "PMID:462521", "title": "Quantitation of carotid stenosis with continuous-wave (C-W) Doppler ultrasound.", "content": "Two methods for determining the degree of stenoses developing on the origin of the internal carotid were tested using non-invasive Doppler ultrasonic imaging (DOPSCAN) of the carotid bifurcations. Spectral analysis of Doppler audio recordings was utilized in determining the maximum frequencies found within the stenosis, as well as the ratio of the frequency downstream to the stenosis, to the frequency within the stenosis. The theoretical relationships between blood flow, velocity, and pressure drop are defined for all grades of stenosis and they predict that carotid flow will not be reduced unless the lumen diameter is less than 1.5 mm. At critical diameter reductions, below 1 mm, the frequencies in human carotids do not exceed 16 KHz because turbulence limits peak velocities. If the maximum systolic frequency exceeds 5 KHZ, when 5 MHZ probes are directed at a 30 degree angle from the body axis, there is always present stenosis up to diameters of less than 3.5 mm by x-ray angiographic measurements. Frequency ratio studies confirm that plaque growth is not symmetrical but they did not improve x-ray angiography correlations because of the limitations of x-ray in measuring cross sectional areas from projection films and limitations of the spot size of x-ray tubes.", "contents": "Quantitation of carotid stenosis with continuous-wave (C-W) Doppler ultrasound. Two methods for determining the degree of stenoses developing on the origin of the internal carotid were tested using non-invasive Doppler ultrasonic imaging (DOPSCAN) of the carotid bifurcations. Spectral analysis of Doppler audio recordings was utilized in determining the maximum frequencies found within the stenosis, as well as the ratio of the frequency downstream to the stenosis, to the frequency within the stenosis. The theoretical relationships between blood flow, velocity, and pressure drop are defined for all grades of stenosis and they predict that carotid flow will not be reduced unless the lumen diameter is less than 1.5 mm. At critical diameter reductions, below 1 mm, the frequencies in human carotids do not exceed 16 KHz because turbulence limits peak velocities. If the maximum systolic frequency exceeds 5 KHZ, when 5 MHZ probes are directed at a 30 degree angle from the body axis, there is always present stenosis up to diameters of less than 3.5 mm by x-ray angiographic measurements. Frequency ratio studies confirm that plaque growth is not symmetrical but they did not improve x-ray angiography correlations because of the limitations of x-ray in measuring cross sectional areas from projection films and limitations of the spot size of x-ray tubes."}
{"id": "PMID:462527", "title": "Preservation of ischaemically injured canine kidneys with hypertonic citrate solution: the importance of mannitol.", "content": "The ability of hypertonic citrate solution to preserve ischaemically injured canine kidneys was investigated. It was found that if mannitol was given i.v. before ischaemia and again after graft revascularization, kidneys subjected to 30 min of warm ischaemia could be preserved for 24 hr, maintaining immediate life-sustaining function.", "contents": "Preservation of ischaemically injured canine kidneys with hypertonic citrate solution: the importance of mannitol. The ability of hypertonic citrate solution to preserve ischaemically injured canine kidneys was investigated. It was found that if mannitol was given i.v. before ischaemia and again after graft revascularization, kidneys subjected to 30 min of warm ischaemia could be preserved for 24 hr, maintaining immediate life-sustaining function."}
{"id": "PMID:462522", "title": "Changes in internal carotid artery flow velocities with cerebral vasodilation and constriction.", "content": "Non-invasive Doppler-shift ultrasound, together with spectral analysis, have been used to study the changes in internal carotid artery flow velocity patterns that occur with cerebral vasoconstriction and vasodilation provoked by over-breathing and breath-holding. Significantly different waveform shapes, characteristic for each vessel, were demonstrated from the internal and external carotid arteries, making identification of the internal carotid certain. In 5 healthy subjects over-breathing for 3 minutes significantly lowered the mean height of the internal carotid waveform by an average of 32% (p less than 0.001). Breath-holding for 40-60 seconds raised the mean height by an average of 31% (p less than 0.001). The pulsatility index of the waveforms varied inversely to mean height. These results show that the effects of cerebral dilation or constriction are easily detected by flow-velocity changes in the internal carotid artery.", "contents": "Changes in internal carotid artery flow velocities with cerebral vasodilation and constriction. Non-invasive Doppler-shift ultrasound, together with spectral analysis, have been used to study the changes in internal carotid artery flow velocity patterns that occur with cerebral vasoconstriction and vasodilation provoked by over-breathing and breath-holding. Significantly different waveform shapes, characteristic for each vessel, were demonstrated from the internal and external carotid arteries, making identification of the internal carotid certain. In 5 healthy subjects over-breathing for 3 minutes significantly lowered the mean height of the internal carotid waveform by an average of 32% (p less than 0.001). Breath-holding for 40-60 seconds raised the mean height by an average of 31% (p less than 0.001). The pulsatility index of the waveforms varied inversely to mean height. These results show that the effects of cerebral dilation or constriction are easily detected by flow-velocity changes in the internal carotid artery."}
{"id": "PMID:462528", "title": "Extracellular fluid of the kidney preserved by the Collins technique.", "content": "Hilar drainage fluid of dog kidneys was analyzed as an approximation to renal extracellular fluid after preservation by flushing with chilled high K-low Na solution (Collins C4) followed by ice-cold storage for 24 and 48 hr in a bath of flushing medium. Compared with the medium, Na and Cl were increased to 30 mM/liter and K decreased slightly to 93 mM/liter. Glucose decreased, whereas lactate, lactic dehydrogenase, and creatine phosphokinase increased by significant amounts in both the drainage fluid and bath. The inulin space of the undrained kidney average 37% of wet weight. Calculated intracellular Na and Cl concentrations averaged 50 and 37 mM/kg cell water while K remained within normal limits. A significant fraction of red blood cells retained during initial flushing entered the effluent during storage. Bath and effluent composition of a human cadaver kidney approximated those of a dog.", "contents": "Extracellular fluid of the kidney preserved by the Collins technique. Hilar drainage fluid of dog kidneys was analyzed as an approximation to renal extracellular fluid after preservation by flushing with chilled high K-low Na solution (Collins C4) followed by ice-cold storage for 24 and 48 hr in a bath of flushing medium. Compared with the medium, Na and Cl were increased to 30 mM/liter and K decreased slightly to 93 mM/liter. Glucose decreased, whereas lactate, lactic dehydrogenase, and creatine phosphokinase increased by significant amounts in both the drainage fluid and bath. The inulin space of the undrained kidney average 37% of wet weight. Calculated intracellular Na and Cl concentrations averaged 50 and 37 mM/kg cell water while K remained within normal limits. A significant fraction of red blood cells retained during initial flushing entered the effluent during storage. Bath and effluent composition of a human cadaver kidney approximated those of a dog."}
{"id": "PMID:462523", "title": "Flow disturbances at the apex and lateral angles of a variety of bifurcation models and their role in development and manifestations of arterial disease.", "content": "Dye flow patterns were studied in 12 glass model bifurcations with angles of 45, 90, 135, and 180 degrees, and area ratios of 0.78, 1.03 and 1.27. At the apex, the dye formed a saddle zone, and streamlines from the core which entered this region were swept over the upper and lower surfaces to enter the lateral angles. Qualitatively, the shape and size of the apex played a key role in this effect. Boundary layer separation occurred in the lateral angles, and increased as flow into the branch was reduced. If the branch was occluded, a complex vortex developed in the first few diameters of the branch, and no flow occurred beyond this, even though the occlusion was about 20 diameters downstream. The results were comparable with steady and pulsatile flow. The implications of these results for the localization of atherosclerosis are discussed.", "contents": "Flow disturbances at the apex and lateral angles of a variety of bifurcation models and their role in development and manifestations of arterial disease. Dye flow patterns were studied in 12 glass model bifurcations with angles of 45, 90, 135, and 180 degrees, and area ratios of 0.78, 1.03 and 1.27. At the apex, the dye formed a saddle zone, and streamlines from the core which entered this region were swept over the upper and lower surfaces to enter the lateral angles. Qualitatively, the shape and size of the apex played a key role in this effect. Boundary layer separation occurred in the lateral angles, and increased as flow into the branch was reduced. If the branch was occluded, a complex vortex developed in the first few diameters of the branch, and no flow occurred beyond this, even though the occlusion was about 20 diameters downstream. The results were comparable with steady and pulsatile flow. The implications of these results for the localization of atherosclerosis are discussed."}
{"id": "PMID:462529", "title": "Improved performance of the isolated rat liver when perfused with purified bovine serum albumin.", "content": "Three groups of isolated rat livers were perfused at 35 C with Krebs-Ringer bicarbonate buffer containing commercial bovine serum albumin (BSA) which had been purified by gel filtration on a column of Sephacryl S-200 and used within 12 hr of purification, or BSA which had been purified by gel filtration and stored at -70 C until used. The ability of livers to produce bile, retain potassium, and to maintain a constant level of glucose in the perfusate was greatly improved in the presence of purified albumin which had not been frozen. Such livers also showed the highest rates of urea synthesis, but the rate of release of aspartate aminotransferase (GOT) from cells and the bile salt content of the bile produced were similar to those found with unpurified BSA. Livers perfused with purified albumin which had been stored in the frozen state were slightly inferior to those perfused with nonfrozen albumin in their ability to produce bile and urea, to retain potassium and GOT within cells, and to maintain a constant concentration of glucose in perfusates. The concentration of bile salts in the bile produced by this group was also lower than that found with the other two groups. Overall, isolated rat livers benefited from perfusion with purified albumin, although freeze storage of this material rendered it slightly inferior to the nonfrozen material in its ability to support the liver.", "contents": "Improved performance of the isolated rat liver when perfused with purified bovine serum albumin. Three groups of isolated rat livers were perfused at 35 C with Krebs-Ringer bicarbonate buffer containing commercial bovine serum albumin (BSA) which had been purified by gel filtration on a column of Sephacryl S-200 and used within 12 hr of purification, or BSA which had been purified by gel filtration and stored at -70 C until used. The ability of livers to produce bile, retain potassium, and to maintain a constant level of glucose in the perfusate was greatly improved in the presence of purified albumin which had not been frozen. Such livers also showed the highest rates of urea synthesis, but the rate of release of aspartate aminotransferase (GOT) from cells and the bile salt content of the bile produced were similar to those found with unpurified BSA. Livers perfused with purified albumin which had been stored in the frozen state were slightly inferior to those perfused with nonfrozen albumin in their ability to produce bile and urea, to retain potassium and GOT within cells, and to maintain a constant concentration of glucose in perfusates. The concentration of bile salts in the bile produced by this group was also lower than that found with the other two groups. Overall, isolated rat livers benefited from perfusion with purified albumin, although freeze storage of this material rendered it slightly inferior to the nonfrozen material in its ability to support the liver."}
{"id": "PMID:462520", "title": "Fluctuation of lipid peroxides and related enzyme activities at time of stroke in stroke-prone spontaneously hypertensive rats.", "content": "The levels of lipid peroxides, determined as thiobarbituric acid reactive substances (TBARS), and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), were examined in the blood from stroke-prone spontaneously hypertensive rats (SHRSP), with and without cerebral lesions, and normotensive Wistar Kyoto (WK) rats. The levels of TBARS in the blood from healthy SHRSP were not significantly different from those of WK rats, while the values of SHRSP (male) with stroke were more than twice as high as those of healthy SHRSP. The activities of SOD and GSH-Px in stroke SHRSP were also statistically different from those of healthy SHRSP.", "contents": "Fluctuation of lipid peroxides and related enzyme activities at time of stroke in stroke-prone spontaneously hypertensive rats. The levels of lipid peroxides, determined as thiobarbituric acid reactive substances (TBARS), and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), were examined in the blood from stroke-prone spontaneously hypertensive rats (SHRSP), with and without cerebral lesions, and normotensive Wistar Kyoto (WK) rats. The levels of TBARS in the blood from healthy SHRSP were not significantly different from those of WK rats, while the values of SHRSP (male) with stroke were more than twice as high as those of healthy SHRSP. The activities of SOD and GSH-Px in stroke SHRSP were also statistically different from those of healthy SHRSP."}
{"id": "PMID:462536", "title": "[Ultrastructure of the DNP fibrils and of the interchromatin granules in isolated nuclei of the rat liver].", "content": "The structural organization of DNP fibrils and interchromatin granules of isolated rat hepatocyte nuclei has been studied in various conditions of chromatin solubilization. When observed either in nuclei fixed in situ or in a solution containing 20 mM TEA and 1 mM MgCl2, a DNP fibril consists of globular structures 20--25 nm in diameter. In the nuclei fixed in a magnesium-free solution (20 mM TA), nucleosome structures are revealed in DNP. Condensation of chromatin results from interaction between 20 nm globular fibrils, whereas the complete dispersion of chromatin is a consequence of its conversion into the nucleosomal form. In the conditions of both DNP structuralization and dispersion, the nuclei are revealed to contain zones of interchromatin granules connected by thin fibrils. It is assumed that the different compactness of these granular-fibrillar complexes and of the regions of condensed chromatin may be used for their separation and fractionation.", "contents": "[Ultrastructure of the DNP fibrils and of the interchromatin granules in isolated nuclei of the rat liver]. The structural organization of DNP fibrils and interchromatin granules of isolated rat hepatocyte nuclei has been studied in various conditions of chromatin solubilization. When observed either in nuclei fixed in situ or in a solution containing 20 mM TEA and 1 mM MgCl2, a DNP fibril consists of globular structures 20--25 nm in diameter. In the nuclei fixed in a magnesium-free solution (20 mM TA), nucleosome structures are revealed in DNP. Condensation of chromatin results from interaction between 20 nm globular fibrils, whereas the complete dispersion of chromatin is a consequence of its conversion into the nucleosomal form. In the conditions of both DNP structuralization and dispersion, the nuclei are revealed to contain zones of interchromatin granules connected by thin fibrils. It is assumed that the different compactness of these granular-fibrillar complexes and of the regions of condensed chromatin may be used for their separation and fractionation."}
{"id": "PMID:462537", "title": "[Cytochemical characteristics of the neurons of the external geniculate body in the rabbit brain in a period of visual function recovery after deprivation].", "content": "To study the properties of the external geniculate body, both neuron protein concentration and content were measured cytophotometrically in the cytoplasm of the above neurons taken from nomal animals and animals transported to normal conditions of illumination only after 2.5 months of visual deprivation, where they were put just after birth. The normalization of protein concentration and content has been observed in the neuron cytoplasm and in the surrounding structures. The magnitude of changes observed was related to the size of the neurons. It is supposed that some portion of neurons may exist in the external geniculate body having different degrees of dependence upon the visual impulsation. Mechanisms of possible rearrangement of the neuron metabolism in the geniculate body during the restoring period are discussed.", "contents": "[Cytochemical characteristics of the neurons of the external geniculate body in the rabbit brain in a period of visual function recovery after deprivation]. To study the properties of the external geniculate body, both neuron protein concentration and content were measured cytophotometrically in the cytoplasm of the above neurons taken from nomal animals and animals transported to normal conditions of illumination only after 2.5 months of visual deprivation, where they were put just after birth. The normalization of protein concentration and content has been observed in the neuron cytoplasm and in the surrounding structures. The magnitude of changes observed was related to the size of the neurons. It is supposed that some portion of neurons may exist in the external geniculate body having different degrees of dependence upon the visual impulsation. Mechanisms of possible rearrangement of the neuron metabolism in the geniculate body during the restoring period are discussed."}
{"id": "PMID:462533", "title": "On schizophrenia prognosis based on data of a clinicogenetical study.", "content": "Peculiarities of the clinical picture of schizophrenia were studied in a group of patients in which less than a half (785) patients had the aggravating fact and in two hereditary-aggravated groups: siblings (109 patients) and parents--children (118 pairs). Prognostic significance of the genetic factor, sex and age was studied as related to peculiarities of the clinical picture of schizophrenia. Hereditary aggravation is shown to be of a decisive importance for schizophrenia prognosis.", "contents": "On schizophrenia prognosis based on data of a clinicogenetical study. Peculiarities of the clinical picture of schizophrenia were studied in a group of patients in which less than a half (785) patients had the aggravating fact and in two hereditary-aggravated groups: siblings (109 patients) and parents--children (118 pairs). Prognostic significance of the genetic factor, sex and age was studied as related to peculiarities of the clinical picture of schizophrenia. Hereditary aggravation is shown to be of a decisive importance for schizophrenia prognosis."}
{"id": "PMID:462538", "title": "[Effect of visual deprivation on the cytochemical differentiation of neurons in the separate laminae of the rabbit superior colliculus].", "content": "The neurons of lamina II, III, VII of the superior colliculus are interferometrically shown to develop features of cytochemical underdevelopment in response to the exclusion of specific visual impulsation. Of the neurons of the lamina studied, lower protein contents in their cytoplasm and nuclei are characteristic, in addition to smaller sizes in comparison with normal ones. A definite specificity of these changes is revealed, which is explained by morphofunctional peculiarities of the neurons of the lamina of the superior colliculus studied.", "contents": "[Effect of visual deprivation on the cytochemical differentiation of neurons in the separate laminae of the rabbit superior colliculus]. The neurons of lamina II, III, VII of the superior colliculus are interferometrically shown to develop features of cytochemical underdevelopment in response to the exclusion of specific visual impulsation. Of the neurons of the lamina studied, lower protein contents in their cytoplasm and nuclei are characteristic, in addition to smaller sizes in comparison with normal ones. A definite specificity of these changes is revealed, which is explained by morphofunctional peculiarities of the neurons of the lamina of the superior colliculus studied."}
{"id": "PMID:462539", "title": "[Effect of the alkylating agent dipin on the induced proliferation of hepatocytes. I. The kinetics of the cell population].", "content": "The alkylating drug dipin was injected to mice 2 hours before a partial hepatectomy. Liver regeneration was characterized by a decrease of the intensity of 3H-thymidine label, an increase of the labeled cell index, absence of mitoses, constant number of binuclear cells. The analysis of these data has shown that dipin causes a sharp (more than by 2 times) increase of the S-period and prolonged (up to 6--20 days) blocking of cells in the G2-period. No phenomenon of unbalanced growth was recorded. No changes in duration of prereplicative period, or in the volume of proliferative pool were recorded. The increase of mitotic cycle periods resulted in the cell population synchronization: by the end of the second ay more than a half of hepatocytes were in S-period, by the end of the third day about 80% of cells passed to G2-period.", "contents": "[Effect of the alkylating agent dipin on the induced proliferation of hepatocytes. I. The kinetics of the cell population]. The alkylating drug dipin was injected to mice 2 hours before a partial hepatectomy. Liver regeneration was characterized by a decrease of the intensity of 3H-thymidine label, an increase of the labeled cell index, absence of mitoses, constant number of binuclear cells. The analysis of these data has shown that dipin causes a sharp (more than by 2 times) increase of the S-period and prolonged (up to 6--20 days) blocking of cells in the G2-period. No phenomenon of unbalanced growth was recorded. No changes in duration of prereplicative period, or in the volume of proliferative pool were recorded. The increase of mitotic cycle periods resulted in the cell population synchronization: by the end of the second ay more than a half of hepatocytes were in S-period, by the end of the third day about 80% of cells passed to G2-period."}
{"id": "PMID:462540", "title": "[Effect of calcium ions on neutral red binding by skeletal muscle plasma membranes and the mechanism of Ca2+ sorption].", "content": "Evidence on the influence of Ca2+ on the binding of neutral red by plasma membranes of skeletal muscle is dealt with. It is suggested that the binding of Ca2+ occurs according to the principle of bimolecular binding. It is suggested that the Ca2+-binding sites with high affinity may control the process of calcium transport by plasma membranes.", "contents": "[Effect of calcium ions on neutral red binding by skeletal muscle plasma membranes and the mechanism of Ca2+ sorption]. Evidence on the influence of Ca2+ on the binding of neutral red by plasma membranes of skeletal muscle is dealt with. It is suggested that the binding of Ca2+ occurs according to the principle of bimolecular binding. It is suggested that the Ca2+-binding sites with high affinity may control the process of calcium transport by plasma membranes."}
{"id": "PMID:462541", "title": "[Destructive changes in the outer perimembrane layers (glycocalyx) of Zajdela ascites hepatoma cells under the action of UV radiation].", "content": "Immediately after far (254) nm and near (300--380 nm) UV light in small and moderate doses alcian blue sorption by glycocalix of Zaidela ascitic hepatoma cells decrease, which is indicative of destruction and solubilization. The effect of UV light on the cell surface is compared with the action of trypsin. Contribution of the damage of outer perimembrane layers to the lethal effect of UV light is discussed.", "contents": "[Destructive changes in the outer perimembrane layers (glycocalyx) of Zajdela ascites hepatoma cells under the action of UV radiation]. Immediately after far (254) nm and near (300--380 nm) UV light in small and moderate doses alcian blue sorption by glycocalix of Zaidela ascitic hepatoma cells decrease, which is indicative of destruction and solubilization. The effect of UV light on the cell surface is compared with the action of trypsin. Contribution of the damage of outer perimembrane layers to the lethal effect of UV light is discussed."}
{"id": "PMID:462542", "title": "[Effect of thyroxine on the duration of the phases of the hepatocyte mitotic cycle at different times of day].", "content": "The lengths of the synthetic phase (S) and postsynthetic gap plus a half of the mitotic time (G2+1/2 M) has been investigated in hepatocytes of control and thyroxine-treated male white rats using percent labeled mitosis curves after injection of isotope at 10, 16, 22 and 4 o'clock. In the control, the minimum lengths of G2 lasted 3.0 hours without being changed during 24 hours. On the contrary, G2+1/2 M and S varied from 3.2 to 4.4 and from 8.0 to 9.5 hours, accordingly. A prolonged administration or hormone induced changes in duration of all the above phases whose alterations in thyroxine-treated group of animals showed 2.0--3.0, 2.9--3.4 and 6.4--11.3 hours, respectively. During 24 hours, there was observed a characteristic pattern of changes in the labeling index (LI) of both groups of animals. It has been established for both the groups that the increased in LI coincides with the shortening of S-phase. The data allow to conclude that some intracycle mechanisms may exist controlling the cell division and exerting their effects on the cells at the end of G1-phase and during G2-phase. Thyroxine is a regulator of cell proliferation, and its effect was found to occur due to the intracycle mechanisms of cell cycle kinetics.", "contents": "[Effect of thyroxine on the duration of the phases of the hepatocyte mitotic cycle at different times of day]. The lengths of the synthetic phase (S) and postsynthetic gap plus a half of the mitotic time (G2+1/2 M) has been investigated in hepatocytes of control and thyroxine-treated male white rats using percent labeled mitosis curves after injection of isotope at 10, 16, 22 and 4 o'clock. In the control, the minimum lengths of G2 lasted 3.0 hours without being changed during 24 hours. On the contrary, G2+1/2 M and S varied from 3.2 to 4.4 and from 8.0 to 9.5 hours, accordingly. A prolonged administration or hormone induced changes in duration of all the above phases whose alterations in thyroxine-treated group of animals showed 2.0--3.0, 2.9--3.4 and 6.4--11.3 hours, respectively. During 24 hours, there was observed a characteristic pattern of changes in the labeling index (LI) of both groups of animals. It has been established for both the groups that the increased in LI coincides with the shortening of S-phase. The data allow to conclude that some intracycle mechanisms may exist controlling the cell division and exerting their effects on the cells at the end of G1-phase and during G2-phase. Thyroxine is a regulator of cell proliferation, and its effect was found to occur due to the intracycle mechanisms of cell cycle kinetics."}
{"id": "PMID:462543", "title": "[Variability of the C-segment sizes of chromosomes 1, 9, 16 and Y in the human chromosome set].", "content": "The investigation of chromosome polymorphism by quantitative methods is a rather hard task. The manual method for measuring C-segments of chromosomes 1, 9, 16 and Y in man is suggested, which is not difficult, being reasonably precise for the population research. Metaphases of the average level of chromosome condensation were taken for analysis. Only the C-segments were measured without measuring chromosomes. The negative chromosome image was 4000-fold magnified, compared to the chromosome natural size, and the boundaries of C-segments of each chromosome were five-fold dotted on a sheet of paper specially printed for this purpose. C-segments were measured by magnifying glass with 0.025 mcm scale unit. For every individuum, C-segments were measured in 5-7 cells only. The data are presented on the estimation of measurement errors and on individual (intercellular) and population (interindividual) variations of C-segments of chromosomes.", "contents": "[Variability of the C-segment sizes of chromosomes 1, 9, 16 and Y in the human chromosome set]. The investigation of chromosome polymorphism by quantitative methods is a rather hard task. The manual method for measuring C-segments of chromosomes 1, 9, 16 and Y in man is suggested, which is not difficult, being reasonably precise for the population research. Metaphases of the average level of chromosome condensation were taken for analysis. Only the C-segments were measured without measuring chromosomes. The negative chromosome image was 4000-fold magnified, compared to the chromosome natural size, and the boundaries of C-segments of each chromosome were five-fold dotted on a sheet of paper specially printed for this purpose. C-segments were measured by magnifying glass with 0.025 mcm scale unit. For every individuum, C-segments were measured in 5-7 cells only. The data are presented on the estimation of measurement errors and on individual (intercellular) and population (interindividual) variations of C-segments of chromosomes."}
{"id": "PMID:462544", "title": "[Effect of the alkylating agent, dipin, on induced hepatocyte proliferation. II. An increase in the DNA synthesis period].", "content": "The alkylating drug dipin was injected to mice 2 hours before a partial hepatectomy. The duration of DNA synthesis of proliferating hepatocytes was determined by means of cytophotometry of DNA mass in the nuclei, labeled with 3H-thymidine 30 hours after the operation. The DNA mass was doubled simultaneously in diploid and tetraploid nuclei within 18-26 hours. The DNA in labeled nuclei of the control mice was doubled in 8 hours. The kinetics of 3H-thymidine incorporation at different stages of S-period was similar in alkylated and normal cells.", "contents": "[Effect of the alkylating agent, dipin, on induced hepatocyte proliferation. II. An increase in the DNA synthesis period]. The alkylating drug dipin was injected to mice 2 hours before a partial hepatectomy. The duration of DNA synthesis of proliferating hepatocytes was determined by means of cytophotometry of DNA mass in the nuclei, labeled with 3H-thymidine 30 hours after the operation. The DNA mass was doubled simultaneously in diploid and tetraploid nuclei within 18-26 hours. The DNA in labeled nuclei of the control mice was doubled in 8 hours. The kinetics of 3H-thymidine incorporation at different stages of S-period was similar in alkylated and normal cells."}
{"id": "PMID:462545", "title": "[Change in the selectivity of the sodium channels of a nerve fiber membrane under the action of veratrine].", "content": "The ionic currents of the nodal membrane were measured under voltage clamp conditions. The membrane being +40 mv. The replacing of the external Na+-ions to K+- and NH4+-ions have showed that the relative pearmeabilities of the veratrine-modified channels calculated from the constant field theory are arranged in the following row: PNa:PK:PNH4 = 1:0.29:0.61, which differs from the same row for the normal channels. The decreasing of the slope of current-voltage relations of the modified channels with the replacing of Na+-ions to K+- and NH4+-ions is the evidence of a more strong binding of these ions to external mouth of the modified channel compared to the binding of Na+-ions.", "contents": "[Change in the selectivity of the sodium channels of a nerve fiber membrane under the action of veratrine]. The ionic currents of the nodal membrane were measured under voltage clamp conditions. The membrane being +40 mv. The replacing of the external Na+-ions to K+- and NH4+-ions have showed that the relative pearmeabilities of the veratrine-modified channels calculated from the constant field theory are arranged in the following row: PNa:PK:PNH4 = 1:0.29:0.61, which differs from the same row for the normal channels. The decreasing of the slope of current-voltage relations of the modified channels with the replacing of Na+-ions to K+- and NH4+-ions is the evidence of a more strong binding of these ions to external mouth of the modified channel compared to the binding of Na+-ions."}
{"id": "PMID:462535", "title": "[Histochemical and ultrastructural characteristics of embryonal hepatocytes exposed to chloridin (pyrimethamine)].", "content": "Single injection of chloridine to rats on the 13th day of pregnancy causes the disturbance of morphofunctional processes in embryo hepatocytes, stimulates granular endoplasmatic reticulum development and glycogen accumulation, increases the number and size of lysosomes, breaks their integrity, produces destructive changes in mitochondria. Activity of lysosomal enzymes with depolymerazing action increases already 30 min after the drug injection, their histopography changes, DNA content decreases.", "contents": "[Histochemical and ultrastructural characteristics of embryonal hepatocytes exposed to chloridin (pyrimethamine)]. Single injection of chloridine to rats on the 13th day of pregnancy causes the disturbance of morphofunctional processes in embryo hepatocytes, stimulates granular endoplasmatic reticulum development and glycogen accumulation, increases the number and size of lysosomes, breaks their integrity, produces destructive changes in mitochondria. Activity of lysosomal enzymes with depolymerazing action increases already 30 min after the drug injection, their histopography changes, DNA content decreases."}
{"id": "PMID:462546", "title": "[Comparative study of the action of procaine and benzocaine on normal and aconitine-modified sodium channels].", "content": "Ionic currents of normal and aconitine modified sodium channels of the Ranvier node membrane were measured under voltage clamp conditions. The experiments with local anesthetics in the external Ringer solution have showed that dissociation constant (Kdis) of normal channel-anesthetic complex for procaine is 0.27 + 0.03 mM, and for benzocaine is 0.68 +/- 0.04 mM. With aconitine modified channels, Kdis increases and becomes 1.32 +/- 0.5 mM and 1.52 +/- 0.3 mM for procaine and benzocaine, respectively. It is ascertained that the development of aconitine effect is inhibited by neutral benzocaine to a lesser extent than by procaine. It is shown that the aconitine effect cannot be reversed by a high concentration of anesthetic. Hence, it appears that aconitine and anesthetic receptors do not coincide.", "contents": "[Comparative study of the action of procaine and benzocaine on normal and aconitine-modified sodium channels]. Ionic currents of normal and aconitine modified sodium channels of the Ranvier node membrane were measured under voltage clamp conditions. The experiments with local anesthetics in the external Ringer solution have showed that dissociation constant (Kdis) of normal channel-anesthetic complex for procaine is 0.27 + 0.03 mM, and for benzocaine is 0.68 +/- 0.04 mM. With aconitine modified channels, Kdis increases and becomes 1.32 +/- 0.5 mM and 1.52 +/- 0.3 mM for procaine and benzocaine, respectively. It is ascertained that the development of aconitine effect is inhibited by neutral benzocaine to a lesser extent than by procaine. It is shown that the aconitine effect cannot be reversed by a high concentration of anesthetic. Hence, it appears that aconitine and anesthetic receptors do not coincide."}
{"id": "PMID:462547", "title": "[Clonal analysis of a transplantable rat seminoma].", "content": "The study of rat testis seminoma by the method of clonal analysis and ectopic transplantations has shown that typical and anaplastic types are different stages of tumor progression in the course of which atypical and anaplastic features of the seminoma cell elements increase. It does not seem inconceivable that the spermacytic form, never encountered during our investigation of 400 tumors, may be an independent seminoma form incapable of transforming into a typical one. A spindle-cell form of seminoma has been described most frequently occurring during transplantation of a typical seminoma into testis and during cloning. The obtained evidence permits to consider the small dark cells of the seminoma as being cambial cells of the tumor which occur abundantly in growing tumors, and are absent in tumors with ceased proloferating. At later stages of the seminoma development, the small dark cells may be absent, their function being performed practically by all tumor cells. Acinar structures found in part of clones from lungs and in the majority of transplants from eye anterior chamber suggest a histogenetic relationship between the typical seminoma and embryocarcinoma, and thus enable us to regard the latter as the most differentiated variants of the seminoma.", "contents": "[Clonal analysis of a transplantable rat seminoma]. The study of rat testis seminoma by the method of clonal analysis and ectopic transplantations has shown that typical and anaplastic types are different stages of tumor progression in the course of which atypical and anaplastic features of the seminoma cell elements increase. It does not seem inconceivable that the spermacytic form, never encountered during our investigation of 400 tumors, may be an independent seminoma form incapable of transforming into a typical one. A spindle-cell form of seminoma has been described most frequently occurring during transplantation of a typical seminoma into testis and during cloning. The obtained evidence permits to consider the small dark cells of the seminoma as being cambial cells of the tumor which occur abundantly in growing tumors, and are absent in tumors with ceased proloferating. At later stages of the seminoma development, the small dark cells may be absent, their function being performed practically by all tumor cells. Acinar structures found in part of clones from lungs and in the majority of transplants from eye anterior chamber suggest a histogenetic relationship between the typical seminoma and embryocarcinoma, and thus enable us to regard the latter as the most differentiated variants of the seminoma."}
{"id": "PMID:462548", "title": "[Photoreactivation of cells and phages injuried by ultraviolet radiation in the ecological long-wave band].", "content": "Photoreactivation (PR) was measured after inactivation by far (254 nm), middle (300-315 nm) and near (315-400 nm) UV radiation of Paramecium caudatum and 8 strains of Escherichia coli differing in PR and dark repair capability. PR volume was high and practically the same after irradiation by far and middle UV, but PR was not observed in near UV-inactivated cells of all the strains. It is proposed that pyrimidine dimers are not significant in near UV lethal lesions in cells, as near UV-irradiated phages (T7 and lambdacI 857) are not photoreactivated in undamaged host bacterial cells.", "contents": "[Photoreactivation of cells and phages injuried by ultraviolet radiation in the ecological long-wave band]. Photoreactivation (PR) was measured after inactivation by far (254 nm), middle (300-315 nm) and near (315-400 nm) UV radiation of Paramecium caudatum and 8 strains of Escherichia coli differing in PR and dark repair capability. PR volume was high and practically the same after irradiation by far and middle UV, but PR was not observed in near UV-inactivated cells of all the strains. It is proposed that pyrimidine dimers are not significant in near UV lethal lesions in cells, as near UV-irradiated phages (T7 and lambdacI 857) are not photoreactivated in undamaged host bacterial cells."}
{"id": "PMID:462549", "title": "[Ultrastructure of tumors arising from Schwann cells].", "content": "Ultrastructural changes have been studied using 67 tumors originated from the Schwann cells. Tumors were induced by means of methylnitrosourea injection with a week intervals. Both benign (fascicular and reticular) and malignant neurinomes were obtained. Main morphological changes were found in the Schwann cells. The tumor cell ultrastructure appeared to be definetely related to the degree of the tumor maturity. The results obtained may suggest that the fine structure of neurogenic tumors is also characteristic of their normal prototype--the Schwann cells.", "contents": "[Ultrastructure of tumors arising from Schwann cells]. Ultrastructural changes have been studied using 67 tumors originated from the Schwann cells. Tumors were induced by means of methylnitrosourea injection with a week intervals. Both benign (fascicular and reticular) and malignant neurinomes were obtained. Main morphological changes were found in the Schwann cells. The tumor cell ultrastructure appeared to be definetely related to the degree of the tumor maturity. The results obtained may suggest that the fine structure of neurogenic tumors is also characteristic of their normal prototype--the Schwann cells."}
{"id": "PMID:462550", "title": "[Autoradiography of lysed L cells with labelled 3H-lysine].", "content": "Using the method of fibr-DNA autoradiography, 3H-lysine labeled L cells were examined. Fibr-labeled structures as long as 400 mkm were revealed. The pattern of labeling along fibres was same as when cells were labeled with 3H-thymidine. In cross experiments when 3H-lysine and 3H-thymidine were used simultaneously, the pattern of labeling remained the same. The data obtained may evidence that the complexation of new synthesized histones with DNA takes place synchronously with the replication, and in the points of chromosome replication.", "contents": "[Autoradiography of lysed L cells with labelled 3H-lysine]. Using the method of fibr-DNA autoradiography, 3H-lysine labeled L cells were examined. Fibr-labeled structures as long as 400 mkm were revealed. The pattern of labeling along fibres was same as when cells were labeled with 3H-thymidine. In cross experiments when 3H-lysine and 3H-thymidine were used simultaneously, the pattern of labeling remained the same. The data obtained may evidence that the complexation of new synthesized histones with DNA takes place synchronously with the replication, and in the points of chromosome replication."}
{"id": "PMID:462551", "title": "[Effect of low-molecular nonelectrolytes on caffeine skeletal muscle contracture in the rat].", "content": "Contractures induced in rat fast (EDL) and slow (SOL) skeletal muscles by 0.03--3 mM of caffeine in conjunction with rapid cooling of muscle from 30 to 0 degrees C (rapid cooling contructures, RCC) were studied. Uprising speed and tension of RCC were dependent on caffeine concentration and cooling gradient. The minimal necessary temperature, below which contractures still developed, was +6 degrees. The initial temperature did not play any important role. Optimal conditions for RCC (when its tension reached 80--200% of twitch) were: cooling from 30 to 0 degrees, and concentrations of caffeine being 5 mM for SOL, and 6--7 mM for EDL. Disruption of T tubules caused by the removal of glycerol and urea (400--600 mM) from muscle fibers did not influence the RCC tension. During the first hour of the removal, relaxation rate of RCC was lowered. In the presence of 400 mM of urea and 600 mM of 1.3-dimethylurea (the latter did not disrupt the T-system), RCC was depressed by 90%, and the rate of tension development was greatly lowered, while twitches remained unchanged. This effects could be reversed during non-electrolyte removal. This may suggest that Ca2+ release is inhibited selectively by urea and by dimethylurea.", "contents": "[Effect of low-molecular nonelectrolytes on caffeine skeletal muscle contracture in the rat]. Contractures induced in rat fast (EDL) and slow (SOL) skeletal muscles by 0.03--3 mM of caffeine in conjunction with rapid cooling of muscle from 30 to 0 degrees C (rapid cooling contructures, RCC) were studied. Uprising speed and tension of RCC were dependent on caffeine concentration and cooling gradient. The minimal necessary temperature, below which contractures still developed, was +6 degrees. The initial temperature did not play any important role. Optimal conditions for RCC (when its tension reached 80--200% of twitch) were: cooling from 30 to 0 degrees, and concentrations of caffeine being 5 mM for SOL, and 6--7 mM for EDL. Disruption of T tubules caused by the removal of glycerol and urea (400--600 mM) from muscle fibers did not influence the RCC tension. During the first hour of the removal, relaxation rate of RCC was lowered. In the presence of 400 mM of urea and 600 mM of 1.3-dimethylurea (the latter did not disrupt the T-system), RCC was depressed by 90%, and the rate of tension development was greatly lowered, while twitches remained unchanged. This effects could be reversed during non-electrolyte removal. This may suggest that Ca2+ release is inhibited selectively by urea and by dimethylurea."}
{"id": "PMID:462552", "title": "[Effect of proteolysis inhibitors on the phagocytic activity and the cytoplasmic enzymatic activity in the leukocytes of white mice].", "content": "The injection of proteolytic inhibitors changes phagocytosis and metabolism of leucocytes in different ways. The polyvalent inhibitor, inhitril, increased phagocytosis but does not change digestive activity of leucocytes. Activities of acid phosphatase, peroxidases and succinatedehydrogenase as well as glycogen contents are seen decreased, whereas activity of glycerophosphate dehydrogenase increases, and that of lactate dehydrogenase does not change. Anti-tryptic serum decreases phagocytosis, both digestive and enzymatic activities of leucocytes, glycogen accumulation being observed. Anti-kallikrein serum decreases phagocytosis and activity of lactate dehydrogenase, and does not change glycogen accumulation and activities of digestion and of acid phosphatase. In addition, activities of succinate-, glycerophosphate dehydrogenase and peroxidase increase.", "contents": "[Effect of proteolysis inhibitors on the phagocytic activity and the cytoplasmic enzymatic activity in the leukocytes of white mice]. The injection of proteolytic inhibitors changes phagocytosis and metabolism of leucocytes in different ways. The polyvalent inhibitor, inhitril, increased phagocytosis but does not change digestive activity of leucocytes. Activities of acid phosphatase, peroxidases and succinatedehydrogenase as well as glycogen contents are seen decreased, whereas activity of glycerophosphate dehydrogenase increases, and that of lactate dehydrogenase does not change. Anti-tryptic serum decreases phagocytosis, both digestive and enzymatic activities of leucocytes, glycogen accumulation being observed. Anti-kallikrein serum decreases phagocytosis and activity of lactate dehydrogenase, and does not change glycogen accumulation and activities of digestion and of acid phosphatase. In addition, activities of succinate-, glycerophosphate dehydrogenase and peroxidase increase."}
{"id": "PMID:462553", "title": "A survey on spontaneous atherosclerosis of camels (Camelus dromedarius) in Iran.", "content": "In a survey carried out on 200 camels (Camelus dromedarius) from different parts of Iran, three cases were diagnosed positive for atherosclerosis on gross and microscopic examination. A sex and age predilection is suspected. The complications of atherosclerosis were not present. To the best of the authors' knowledge this is the first report of atherosclerosis in camels.", "contents": "A survey on spontaneous atherosclerosis of camels (Camelus dromedarius) in Iran. In a survey carried out on 200 camels (Camelus dromedarius) from different parts of Iran, three cases were diagnosed positive for atherosclerosis on gross and microscopic examination. A sex and age predilection is suspected. The complications of atherosclerosis were not present. To the best of the authors' knowledge this is the first report of atherosclerosis in camels."}
{"id": "PMID:462554", "title": "Crossbreeding of exotic and indigenous cattle in Nigeria--haematological correlates.", "content": "The blood values of the German Brown, N'Dama and their three-quarter, half and quarter hybrids were studied to ascertain if and how some haematological parameters changed with crossbreeding. It was found that the red blood cell (RBC) and white blood cell (WBC) count and packed cell volume percentage (PCV) were significantly higher (P less than 0.01) in the N'Dama than in the German Brown; the crosses had values which were intermediate as compared to the two parent breeds. Generally, the changes in the mean blood values paralleled changes in the expected genotypes resulting from crossbreeding of the two parent breeds. The tendency of the blood values to change in direct proportion to the degree of N'Dama contribution was strongest for the WBC, followed by the PCV and RBC, respectively. A small random sample for leucocyte differential count did not reveal any differences amongst the genotypic groups.", "contents": "Crossbreeding of exotic and indigenous cattle in Nigeria--haematological correlates. The blood values of the German Brown, N'Dama and their three-quarter, half and quarter hybrids were studied to ascertain if and how some haematological parameters changed with crossbreeding. It was found that the red blood cell (RBC) and white blood cell (WBC) count and packed cell volume percentage (PCV) were significantly higher (P less than 0.01) in the N'Dama than in the German Brown; the crosses had values which were intermediate as compared to the two parent breeds. Generally, the changes in the mean blood values paralleled changes in the expected genotypes resulting from crossbreeding of the two parent breeds. The tendency of the blood values to change in direct proportion to the degree of N'Dama contribution was strongest for the WBC, followed by the PCV and RBC, respectively. A small random sample for leucocyte differential count did not reveal any differences amongst the genotypic groups."}
{"id": "PMID:462558", "title": "Experimental infection of domestic pigs with the virus of peste des petits ruminants.", "content": "Pigs could be subclinically infected with PPRV by inoculation or contact with infected goats. There was no evidence that the virus could spread to uninfected pigs or goats and pigs are not considered important in the epidemiology of PPRV.", "contents": "Experimental infection of domestic pigs with the virus of peste des petits ruminants. Pigs could be subclinically infected with PPRV by inoculation or contact with infected goats. There was no evidence that the virus could spread to uninfected pigs or goats and pigs are not considered important in the epidemiology of PPRV."}
{"id": "PMID:462559", "title": "Oxfendazole--anthelmintic activity in Egyptian goats artificially infected with gastrointestinal nematodes.", "content": "The recently developed benzimidazole anthelmintic, oxfendazole, was tested against artificial nematode infestations in Egyptian goats using oral dosing at 4.5 and 2.8 mg/kg. A 100% clearance of mature and immature Haemonchus contortus, Trichostrongylus axei, Ostertagia circumcincta, Coopera curticei, Bunostomum trigonocephalum and Chabertia ovina was obtained at the 4.5 mg/kg level. Very high levels of clearance against the mature worms were obtained at 2.8 mg/kg but the drug was less effective against immature worms at the lower dose rate. PCV, hemoglobin concentration and total erythrocyte counts declined after infection but became significantly (P less than 0.001) raised in treated animal.", "contents": "Oxfendazole--anthelmintic activity in Egyptian goats artificially infected with gastrointestinal nematodes. The recently developed benzimidazole anthelmintic, oxfendazole, was tested against artificial nematode infestations in Egyptian goats using oral dosing at 4.5 and 2.8 mg/kg. A 100% clearance of mature and immature Haemonchus contortus, Trichostrongylus axei, Ostertagia circumcincta, Coopera curticei, Bunostomum trigonocephalum and Chabertia ovina was obtained at the 4.5 mg/kg level. Very high levels of clearance against the mature worms were obtained at 2.8 mg/kg but the drug was less effective against immature worms at the lower dose rate. PCV, hemoglobin concentration and total erythrocyte counts declined after infection but became significantly (P less than 0.001) raised in treated animal."}
{"id": "PMID:462561", "title": "A preliminary investigation into the control of trichostrongylosis in calves in Kenya.", "content": "A series of 3 grazing experiments was carried out to collect data relevant for designing techniques to prevent trichostrongylosis in dairy calves in Kenya. In the first experiment 1 week in/5 weeks out rotation was compared with set stocking. The results indicated that rotation is not an effective control measure. In the second experiment the effects of daily removal of faeces from the field were investigated. This practice also had a limited effect in controlling nematode infection in calves. The third experiment concentrated on the influence of nutrition level. It was shown that feeding level does affect the growth rate but its effect on the course of the egg output was not clear-cut. The results also indicated that the dry season greatly reduces herbage infestation.", "contents": "A preliminary investigation into the control of trichostrongylosis in calves in Kenya. A series of 3 grazing experiments was carried out to collect data relevant for designing techniques to prevent trichostrongylosis in dairy calves in Kenya. In the first experiment 1 week in/5 weeks out rotation was compared with set stocking. The results indicated that rotation is not an effective control measure. In the second experiment the effects of daily removal of faeces from the field were investigated. This practice also had a limited effect in controlling nematode infection in calves. The third experiment concentrated on the influence of nutrition level. It was shown that feeding level does affect the growth rate but its effect on the course of the egg output was not clear-cut. The results also indicated that the dry season greatly reduces herbage infestation."}
{"id": "PMID:462565", "title": "Acute diesel fuel poisoning in goats.", "content": "The clinical, haematological and pathological features of an outbreak of diesel fuel poisoning in a group of goats are described. The major clinical signs were dullness, pneumonia and nervous signs.", "contents": "Acute diesel fuel poisoning in goats. The clinical, haematological and pathological features of an outbreak of diesel fuel poisoning in a group of goats are described. The major clinical signs were dullness, pneumonia and nervous signs."}
{"id": "PMID:462566", "title": "Study of women who did not respond to screening for cervical cancer.", "content": "Demographic and social factors influencing the population response to cervical screening programs have been studied. Age, marital status and, to a lesser extent, place of birth and socio-economic status were the most relevant factors. On the other hand, the reasons for nonparticipation were mainly the lack of information and motivation. Personal invitations, the recall of women who did not present on the first call, and the setting up of decentralized smear collection clinics proved to be useful tools to increase attendance.", "contents": "Study of women who did not respond to screening for cervical cancer. Demographic and social factors influencing the population response to cervical screening programs have been studied. Age, marital status and, to a lesser extent, place of birth and socio-economic status were the most relevant factors. On the other hand, the reasons for nonparticipation were mainly the lack of information and motivation. Personal invitations, the recall of women who did not present on the first call, and the setting up of decentralized smear collection clinics proved to be useful tools to increase attendance."}
{"id": "PMID:462567", "title": "Histopathological evidence in support of the association of elevated proton spin-lattice relaxation times with the malignant state.", "content": "The pulsed nuclear magnetic resonance technique was explored for its potential diagnostic value in human cancer. Measurements of proton spin-lattice relaxation times (T1) of cellular water protons of normal and malignant esophageal tissues showed elevated T1 values in the latter. In some cases, tissues which appeared normal on gross examination assumed as uninvolved tissues had T1 values higher than the other grossly uninvolved tissues and often closer to the T1 of the corresponding tumor tissue. A histopathological study of the assumed uninvolved areas also studied for the T1 values was therefore undertaken. A preliminary study demonstrated the presence of malignant cell groups or clusters in some of the uninvolved samples with higher T1 compared to the true uninvolved tissues, which had a normal histological picture and low T1 values. This observation has brought out the importance of histopathological studies in addition to relaxation studies to comprehend contributory factors to relaxation. Secondly, it lends support to the thesis of elevated T1 values being characteristics of the malignant state.", "contents": "Histopathological evidence in support of the association of elevated proton spin-lattice relaxation times with the malignant state. The pulsed nuclear magnetic resonance technique was explored for its potential diagnostic value in human cancer. Measurements of proton spin-lattice relaxation times (T1) of cellular water protons of normal and malignant esophageal tissues showed elevated T1 values in the latter. In some cases, tissues which appeared normal on gross examination assumed as uninvolved tissues had T1 values higher than the other grossly uninvolved tissues and often closer to the T1 of the corresponding tumor tissue. A histopathological study of the assumed uninvolved areas also studied for the T1 values was therefore undertaken. A preliminary study demonstrated the presence of malignant cell groups or clusters in some of the uninvolved samples with higher T1 compared to the true uninvolved tissues, which had a normal histological picture and low T1 values. This observation has brought out the importance of histopathological studies in addition to relaxation studies to comprehend contributory factors to relaxation. Secondly, it lends support to the thesis of elevated T1 values being characteristics of the malignant state."}
{"id": "PMID:462568", "title": "Preliminary study on the carcinogenic activity of the fungicide manganese ethylenebisdithiocarbamate in the adult newt. Triturus cristatus carnifex.", "content": "Percutaneous exposure of adult newts to the fungicide manganese ethylenebisdithiocarbamate (maneb) at 3 concentration levels (0.5, 2.5 and 5.0 ppm) for a total of 19.23 weeks did not result in the induction of any neoplasms.", "contents": "Preliminary study on the carcinogenic activity of the fungicide manganese ethylenebisdithiocarbamate in the adult newt. Triturus cristatus carnifex. Percutaneous exposure of adult newts to the fungicide manganese ethylenebisdithiocarbamate (maneb) at 3 concentration levels (0.5, 2.5 and 5.0 ppm) for a total of 19.23 weeks did not result in the induction of any neoplasms."}
{"id": "PMID:462569", "title": "Cyclophosphamide and iphosphamide against Lewis lung carcinoma: evaluation of toxic and therapeutic effects.", "content": "In the present report, investigations have been carried out to evaluate toxic and therapeutic effects of cyclophosphamide vs its isomer iphosphamide. Cytostatic action of the 2 drugs was assayed on the murine Lewis lung carcinoma (3LL). It has been observed that iphosphamide is less toxic as compared to cyclophosphamide (LD50 IP LD50 CP = 1.5); on the other hand, to reach the same therapeutic effectiveness on 3LL, an iphosphamide dose 1.6 - 2 times higher than that of its parent compound is necessary.", "contents": "Cyclophosphamide and iphosphamide against Lewis lung carcinoma: evaluation of toxic and therapeutic effects. In the present report, investigations have been carried out to evaluate toxic and therapeutic effects of cyclophosphamide vs its isomer iphosphamide. Cytostatic action of the 2 drugs was assayed on the murine Lewis lung carcinoma (3LL). It has been observed that iphosphamide is less toxic as compared to cyclophosphamide (LD50 IP LD50 CP = 1.5); on the other hand, to reach the same therapeutic effectiveness on 3LL, an iphosphamide dose 1.6 - 2 times higher than that of its parent compound is necessary."}
{"id": "PMID:462570", "title": "Endocrine differentiation in basal cell carcinoma.", "content": "Argyrophilic cells have been found in 2 different basal cell carcinomas (BCC), and membrane-bound endocrine-like granules, in the carcinoid range, have been observed in 4 consecutive cases of such tumors. It is postulated that BCC can occasionally display cells showing an endocrine differentiation.", "contents": "Endocrine differentiation in basal cell carcinoma. Argyrophilic cells have been found in 2 different basal cell carcinomas (BCC), and membrane-bound endocrine-like granules, in the carcinoid range, have been observed in 4 consecutive cases of such tumors. It is postulated that BCC can occasionally display cells showing an endocrine differentiation."}
{"id": "PMID:462571", "title": "Clear cell carcinoma of endometrium.", "content": "Three cases of clear cell carcinoma of the endometrium are here reported. Clear cell carcinoma is a rare and aggressive type of endometrial adenocarcinoma. Patients are older and are in the menopause much longer than are patients with typical endometrial carcinoma. This fact may reflect the presence of counterbalanced sex hormones in the process of carcinogenesis.", "contents": "Clear cell carcinoma of endometrium. Three cases of clear cell carcinoma of the endometrium are here reported. Clear cell carcinoma is a rare and aggressive type of endometrial adenocarcinoma. Patients are older and are in the menopause much longer than are patients with typical endometrial carcinoma. This fact may reflect the presence of counterbalanced sex hormones in the process of carcinogenesis."}
{"id": "PMID:462572", "title": "Prognostic value of the Kiel classification of malignant non-Hodgkin's lymphomas.", "content": "The aim of the present research was to evaluate the prognostic value of the Kiel classification of malignant non-Hodgkin's lymphomas. For this purpose a series of 100 consecutive, previously untreated adults with advanced malignant non-Hodgkin's lymphomas was analyzed. The median age of the patients was 54 years; 61 patients were males. Although the number of the various groups considered was limited, a statistically significant difference (p less than 0.001) was found in the median survival of patients with lymphomas of low-grade malignancy (lymphocytic, lymphoplasmacytoid, centrocytic, centroblastic-centrocytic lymphoma) and lymphomas of high-grade malignancy (centroblastic, lymphoblastic, immunoblastic lymphoma). A difference in survival (p less than 0.001) was also observed among the patients with lymphocytic lymphoma and those with centroblastic-centrocytic lymphoma, whereas no significant difference in survival was found between the histological subtypes of high-grade malignant lymphomas. Our observations support the opinion that the Kiel classification is useful in clinical practice to distinguish the histological types with a better prognosis from those with a worse one; in addition this classification appears to be of conceptual value.", "contents": "Prognostic value of the Kiel classification of malignant non-Hodgkin's lymphomas. The aim of the present research was to evaluate the prognostic value of the Kiel classification of malignant non-Hodgkin's lymphomas. For this purpose a series of 100 consecutive, previously untreated adults with advanced malignant non-Hodgkin's lymphomas was analyzed. The median age of the patients was 54 years; 61 patients were males. Although the number of the various groups considered was limited, a statistically significant difference (p less than 0.001) was found in the median survival of patients with lymphomas of low-grade malignancy (lymphocytic, lymphoplasmacytoid, centrocytic, centroblastic-centrocytic lymphoma) and lymphomas of high-grade malignancy (centroblastic, lymphoblastic, immunoblastic lymphoma). A difference in survival (p less than 0.001) was also observed among the patients with lymphocytic lymphoma and those with centroblastic-centrocytic lymphoma, whereas no significant difference in survival was found between the histological subtypes of high-grade malignant lymphomas. Our observations support the opinion that the Kiel classification is useful in clinical practice to distinguish the histological types with a better prognosis from those with a worse one; in addition this classification appears to be of conceptual value."}
{"id": "PMID:462573", "title": "Hepatic enzymes in Hodgkin's and non Hodgkin's lymphoma.", "content": "Serum alkaline phosphatase levels in patients with Hodgkin's and non-Hodgkin's lymphoma were studied. The findings were correlated with clinical stage, particularly hepatic involvement, and histologic findings. Serum levels of other hepatic enzymes (SGOT, 5,N and gamma GT) were also measured. The usefulness of these studies for clinical staging was described, as well as speculation on the observed differences in Hodgkin's and non-Hodgkin's patients.", "contents": "Hepatic enzymes in Hodgkin's and non Hodgkin's lymphoma. Serum alkaline phosphatase levels in patients with Hodgkin's and non-Hodgkin's lymphoma were studied. The findings were correlated with clinical stage, particularly hepatic involvement, and histologic findings. Serum levels of other hepatic enzymes (SGOT, 5,N and gamma GT) were also measured. The usefulness of these studies for clinical staging was described, as well as speculation on the observed differences in Hodgkin's and non-Hodgkin's patients."}
{"id": "PMID:462574", "title": "Clinical data and estradiol receptor evaluation in breast cancer biopsies.", "content": "To determine the correlation between the response to the estradiol receptor test and some fundamental properties of the tumor and the host, a study was conducted on 337 patients. Primary carcinomas, including lobular carcinomas, and recurrences or metastases are characterized by similar frequencies of response to the estradiol receptor test (68% positive, 26% negative and 6% borderline), while normal breast tissue is characterized by a higher frequency of negative results (3% positive, 71% negative and 26% borderline). Postmenopausal patients show a shift of estradiol receptor levels towards higher values with respect to premenopausal patients. Contemporaneously, an increase in estradiol receptor concentrations with patient age is observed. The clinical stage appears in influence estradiol receptor content only in postmenopausal patients, for whom an increase in the tumor size is accompanied by a decrease in the estradiol receptor levels. The apparent association constant of the receptors assumes values ranging from 1.5 to about 300 X 10(9) M-1 and does not appear to be related to either the type of tumor tissue or to the clinical stage of the tumor, age, or menopausal status of the patients.", "contents": "Clinical data and estradiol receptor evaluation in breast cancer biopsies. To determine the correlation between the response to the estradiol receptor test and some fundamental properties of the tumor and the host, a study was conducted on 337 patients. Primary carcinomas, including lobular carcinomas, and recurrences or metastases are characterized by similar frequencies of response to the estradiol receptor test (68% positive, 26% negative and 6% borderline), while normal breast tissue is characterized by a higher frequency of negative results (3% positive, 71% negative and 26% borderline). Postmenopausal patients show a shift of estradiol receptor levels towards higher values with respect to premenopausal patients. Contemporaneously, an increase in estradiol receptor concentrations with patient age is observed. The clinical stage appears in influence estradiol receptor content only in postmenopausal patients, for whom an increase in the tumor size is accompanied by a decrease in the estradiol receptor levels. The apparent association constant of the receptors assumes values ranging from 1.5 to about 300 X 10(9) M-1 and does not appear to be related to either the type of tumor tissue or to the clinical stage of the tumor, age, or menopausal status of the patients."}
{"id": "PMID:462575", "title": "A preliminary report of a pilot trial in adjuvant chemotherapy of primary melanoma.", "content": "It is well known that level of skin invasion and tumor thickness are significant prognostic factors in the evolution of primary melanoma. The prognosis of primary melanoma Clark III to V skin invasion level and more than 1.5 mm thick confirms this statement. Even the prophylactic dissection of regional lymph nodes has not improved results. In an attempt to obtain better results in the treatment of primary melanomas, a pilot trial was carried out combining surgery and adjuvant chemotherapy. A group of 21 patients with Clark III, IV and V level primary melanoma who underwent adjuvant polychemotherapy (velba + dactinomycin + procarbazine) for 1 year after surgery showed a very low incidence of recurrences (5%) after 24 months of observation. The historical control group, with the same level of tumor skin invasion, treated only surgically had in the same follow-up period a recurrence rate of 65%. This difference was statistically significant (p less than 0.01). All patients who received adjuvant chemotherapy survived 2 years whereas survival was 77% (p less than 0.05) in the surgical historical control group. Favorable results with the same protocol of adjuvant chemotherapy were not obtained in the group of 16 patients with stage II melanoma when compared with primary tumors. However, 4 recurrences were observed after 12 months of observation; toxic side effects of adjuvant chemotherapy were mild and tolerable. Considering the insufficient number of clinical trials with adjuvant chemotherapy, as well as sometimes controversial results, further randomized clinical studies are needed to establish the actual value of this conbined method in the treatment of primary melanoma with a high risk of dissemination.", "contents": "A preliminary report of a pilot trial in adjuvant chemotherapy of primary melanoma. It is well known that level of skin invasion and tumor thickness are significant prognostic factors in the evolution of primary melanoma. The prognosis of primary melanoma Clark III to V skin invasion level and more than 1.5 mm thick confirms this statement. Even the prophylactic dissection of regional lymph nodes has not improved results. In an attempt to obtain better results in the treatment of primary melanomas, a pilot trial was carried out combining surgery and adjuvant chemotherapy. A group of 21 patients with Clark III, IV and V level primary melanoma who underwent adjuvant polychemotherapy (velba + dactinomycin + procarbazine) for 1 year after surgery showed a very low incidence of recurrences (5%) after 24 months of observation. The historical control group, with the same level of tumor skin invasion, treated only surgically had in the same follow-up period a recurrence rate of 65%. This difference was statistically significant (p less than 0.01). All patients who received adjuvant chemotherapy survived 2 years whereas survival was 77% (p less than 0.05) in the surgical historical control group. Favorable results with the same protocol of adjuvant chemotherapy were not obtained in the group of 16 patients with stage II melanoma when compared with primary tumors. However, 4 recurrences were observed after 12 months of observation; toxic side effects of adjuvant chemotherapy were mild and tolerable. Considering the insufficient number of clinical trials with adjuvant chemotherapy, as well as sometimes controversial results, further randomized clinical studies are needed to establish the actual value of this conbined method in the treatment of primary melanoma with a high risk of dissemination."}
{"id": "PMID:462576", "title": "17 beta-estradiol, 5 alpha-dihydrotestosterone, progesterone and cortisol receptors in normal and neoplastic human endometrium.", "content": "Using the value of 0.24 fmoles/microgram DNA as breaking point between high and low binding capacities, we quantified receptors for 17 beta-estradiol (ER), 5 alpha-dihydrotestosterone (DHTR), progesterone (PR) and cortisol (CR) in normal and neoplastic human uterine tissues. Concerning receptors occurrence, significant relationships were observed between ER and PR, ER and DHTR, and DHTR and PR. A direct correlation between the presence of ER and tumor grading was found: PR was less frequent in grade II and absent in grade III endometrial carcinoma, however this was not a significant correlation. In endometrial carcinoma at least 1 of the receptors was detected in 67-91% of the cases, 3 receptors (ER, DHTR, PR) in 56%, and all 4 receptors in 45%. The simultaneous detection of multiple receptors could play an important role in determining hormone response.", "contents": "17 beta-estradiol, 5 alpha-dihydrotestosterone, progesterone and cortisol receptors in normal and neoplastic human endometrium. Using the value of 0.24 fmoles/microgram DNA as breaking point between high and low binding capacities, we quantified receptors for 17 beta-estradiol (ER), 5 alpha-dihydrotestosterone (DHTR), progesterone (PR) and cortisol (CR) in normal and neoplastic human uterine tissues. Concerning receptors occurrence, significant relationships were observed between ER and PR, ER and DHTR, and DHTR and PR. A direct correlation between the presence of ER and tumor grading was found: PR was less frequent in grade II and absent in grade III endometrial carcinoma, however this was not a significant correlation. In endometrial carcinoma at least 1 of the receptors was detected in 67-91% of the cases, 3 receptors (ER, DHTR, PR) in 56%, and all 4 receptors in 45%. The simultaneous detection of multiple receptors could play an important role in determining hormone response."}
{"id": "PMID:462577", "title": "Xeroradiography of bone and soft tissue tumors.", "content": "Xeroradiography was used for the study of 233 cases of bone and soft tissue tumors, both benign and malignant, 177 of which were histologically proven. In 150 cases, comparative x-ray films were also available. In bone tumors, xeroradiography was more effective than film radiography in 33 cases (44%), less effective in 16 cases (22%) and the 2 techniques were substantially equivalent in 25 cases (34%). In soft tissue tumors, xeroradiography was more effective than film radiography in 59 cases (78%), less effective in 2 cases (3%) and equivalent in 15 cases (19%). Xeroradiography is held to be a useful second-line technique in selected bone tumors, in particular for the study of trabecular structure, periosteal calcifications and cortical bone. In the field of soft tissue tumors, xeroradiography can be a promising first-choice examination in the presence of a mass or when a recurrence is suspected. The advantages and drawbacks of this technique in both types of tumors are reviewed and discussed.", "contents": "Xeroradiography of bone and soft tissue tumors. Xeroradiography was used for the study of 233 cases of bone and soft tissue tumors, both benign and malignant, 177 of which were histologically proven. In 150 cases, comparative x-ray films were also available. In bone tumors, xeroradiography was more effective than film radiography in 33 cases (44%), less effective in 16 cases (22%) and the 2 techniques were substantially equivalent in 25 cases (34%). In soft tissue tumors, xeroradiography was more effective than film radiography in 59 cases (78%), less effective in 2 cases (3%) and equivalent in 15 cases (19%). Xeroradiography is held to be a useful second-line technique in selected bone tumors, in particular for the study of trabecular structure, periosteal calcifications and cortical bone. In the field of soft tissue tumors, xeroradiography can be a promising first-choice examination in the presence of a mass or when a recurrence is suspected. The advantages and drawbacks of this technique in both types of tumors are reviewed and discussed."}
{"id": "PMID:462578", "title": "Histopathological study of an imperfect type ganglioneuroblastoma.", "content": "Based on their observations, the authors stress the occurrence of 2 histological varieties of ganglioneuroblastoma; the former characterized by a combination of undifferentiated with mature typical ganglion cells, the latter revealing a combination of undifferentiated with pleomorphic atypical cells. After demonstrating the ganglion origin of pleomorphic cells, attention is focused on the difficulties involved in the diagnosis of the second type of ganglioneuroblastoma, which in the author's view should be included in the present classifications of ANS tumors.", "contents": "Histopathological study of an imperfect type ganglioneuroblastoma. Based on their observations, the authors stress the occurrence of 2 histological varieties of ganglioneuroblastoma; the former characterized by a combination of undifferentiated with mature typical ganglion cells, the latter revealing a combination of undifferentiated with pleomorphic atypical cells. After demonstrating the ganglion origin of pleomorphic cells, attention is focused on the difficulties involved in the diagnosis of the second type of ganglioneuroblastoma, which in the author's view should be included in the present classifications of ANS tumors."}
{"id": "PMID:462579", "title": "[Findings on the knowledge of self-examination of the breast].", "content": "A research on the degree of knowledge about the problem of breast self-examination was carried out in a group of 500 women in the Out-patients Department of the Cancer Institute in Milan. The women were given a questionnaire to fill up at home and to return at the moment of the medical examination. The analysis of data showed that, though the level of education of the subjects was fairly good, the knowledge and the practise of the periodical self-examination as a method for an early diagnosis was very low. 70% of the women declared to have some knowledge about the method, but only 9% declared to practise it regularly.", "contents": "[Findings on the knowledge of self-examination of the breast]. A research on the degree of knowledge about the problem of breast self-examination was carried out in a group of 500 women in the Out-patients Department of the Cancer Institute in Milan. The women were given a questionnaire to fill up at home and to return at the moment of the medical examination. The analysis of data showed that, though the level of education of the subjects was fairly good, the knowledge and the practise of the periodical self-examination as a method for an early diagnosis was very low. 70% of the women declared to have some knowledge about the method, but only 9% declared to practise it regularly."}
{"id": "PMID:462580", "title": "Effects of some pyrrolic and dihydropyrrolizine esters on mouse skin: a preliminary study.", "content": "Groups of male BALB/c mice were given 33 to 35 treatments during 52 to 54 weeks with 5 ester alkylating agents. Two of these (I and II) were potential metabolites of carcinogenic pyrrolizidine alkaloids; the others (III-V) were chemically similar synthetic compounds. Each dose of 0.5 or 1.0 mumol, in acetone, was applied to the shaved dorsal skin. A control group received acetone. Three skin tumours and 2 preneoplastic lesions were found among 9 mice receiving the compound IV, the agent giving the slowest chemical alkylation reactions. No skin tumours were seen in the other groups. The results suggest that the relationship between carcinogenic potential and alkylating reactivity in this series of compounds should be further investigated.", "contents": "Effects of some pyrrolic and dihydropyrrolizine esters on mouse skin: a preliminary study. Groups of male BALB/c mice were given 33 to 35 treatments during 52 to 54 weeks with 5 ester alkylating agents. Two of these (I and II) were potential metabolites of carcinogenic pyrrolizidine alkaloids; the others (III-V) were chemically similar synthetic compounds. Each dose of 0.5 or 1.0 mumol, in acetone, was applied to the shaved dorsal skin. A control group received acetone. Three skin tumours and 2 preneoplastic lesions were found among 9 mice receiving the compound IV, the agent giving the slowest chemical alkylation reactions. No skin tumours were seen in the other groups. The results suggest that the relationship between carcinogenic potential and alkylating reactivity in this series of compounds should be further investigated."}
{"id": "PMID:462581", "title": "Macrophages in skin cancer: quantitative and functional studies.", "content": "The number of tumor-infiltrating macrophages was estimated in 43 patients with skin cancer, including 18 cases of squamous cell and 25 cases of basal cell carcinoma. Macrophages were identified in cell cultures by 2 assays, namely phagocytosis and resistance to detachment by trypsin. The average percentage of adherent cells for the 2 groups of skin tumors was 4.5 +/- 2.6 and 10.2 +/- 5.2, respectively, and the difference was statistically significant. Follow-up studies after surgical excision of the primary neoplasm showed a relatively low macrophage content in 2 of the 4 cases in which local recurrences occurred. Preliminary functional studies suggested that soluble factors may be released by neoplastic cells, accounting for the inhibitory effect of tumor cell supernatants on macrophage chemotaxis in vitro.", "contents": "Macrophages in skin cancer: quantitative and functional studies. The number of tumor-infiltrating macrophages was estimated in 43 patients with skin cancer, including 18 cases of squamous cell and 25 cases of basal cell carcinoma. Macrophages were identified in cell cultures by 2 assays, namely phagocytosis and resistance to detachment by trypsin. The average percentage of adherent cells for the 2 groups of skin tumors was 4.5 +/- 2.6 and 10.2 +/- 5.2, respectively, and the difference was statistically significant. Follow-up studies after surgical excision of the primary neoplasm showed a relatively low macrophage content in 2 of the 4 cases in which local recurrences occurred. Preliminary functional studies suggested that soluble factors may be released by neoplastic cells, accounting for the inhibitory effect of tumor cell supernatants on macrophage chemotaxis in vitro."}
{"id": "PMID:462582", "title": "Correlation between clinical response to bilateral oophorectomy, estrogen receptors and urinary androgen excretion in 49 patients with advanced breast cancer.", "content": "The aim of this study was to find out if it is possible to predict the clinical response to bilateral oophorectomy in premenopausal patients with advanced breast cancer. Two methods of determination were used before oophorectomy: 1) the presence of estrogen receptors in the tumor tissue; 2) the urinary concentration of androgens. The clinical response to oophorectomy was evaluated after a six-month follow-up. Determinations carried out on 49 patients showed that a significant correlation exists between clinical response to oophorectomy and androgenic activity alone or in combination with estrogen receptors when both tests give concordant results.", "contents": "Correlation between clinical response to bilateral oophorectomy, estrogen receptors and urinary androgen excretion in 49 patients with advanced breast cancer. The aim of this study was to find out if it is possible to predict the clinical response to bilateral oophorectomy in premenopausal patients with advanced breast cancer. Two methods of determination were used before oophorectomy: 1) the presence of estrogen receptors in the tumor tissue; 2) the urinary concentration of androgens. The clinical response to oophorectomy was evaluated after a six-month follow-up. Determinations carried out on 49 patients showed that a significant correlation exists between clinical response to oophorectomy and androgenic activity alone or in combination with estrogen receptors when both tests give concordant results."}
{"id": "PMID:462583", "title": "Variations in serum copper and ceruloplasmin levels in advanced gastrointestinal cancer treated with polychemotherapy.", "content": "Serum copper and ceruloplasmin levels (SCL, SCeL) in 57 patients with advanced cancer of the stomach (35 cases) or large intestine (22 cases) treated with polychemotherapy were studies. In gastroenteric cancer, SCL, which are already high in untreated patients, have a tendency to increase further in cases of progression of the disease, while they seem to significantly decrease in cases of remission. SCeL during the trial appeared to be correlated to the clinical evolution of the disease only in the case of stomach cancer. In large intestine cancer, SCeL did not show any significant variation in relation to the normal range. These observations, in particular on the behavior of SCL in the neoplasms of the digestive tract, are in accordance with the results of other studies. The authors are inclined to attach a diagnostic and prognostic value to the variation in SCL and SCeL in gastrointestinal cancer.", "contents": "Variations in serum copper and ceruloplasmin levels in advanced gastrointestinal cancer treated with polychemotherapy. Serum copper and ceruloplasmin levels (SCL, SCeL) in 57 patients with advanced cancer of the stomach (35 cases) or large intestine (22 cases) treated with polychemotherapy were studies. In gastroenteric cancer, SCL, which are already high in untreated patients, have a tendency to increase further in cases of progression of the disease, while they seem to significantly decrease in cases of remission. SCeL during the trial appeared to be correlated to the clinical evolution of the disease only in the case of stomach cancer. In large intestine cancer, SCeL did not show any significant variation in relation to the normal range. These observations, in particular on the behavior of SCL in the neoplasms of the digestive tract, are in accordance with the results of other studies. The authors are inclined to attach a diagnostic and prognostic value to the variation in SCL and SCeL in gastrointestinal cancer."}
{"id": "PMID:462584", "title": "Combination chemotherapy with 5-fluorouracil and methyl-CCNU for the treatment of advanced gastrointestinal cancer.", "content": "Sixteen patients with advanced gastrointestinal cancer (colorectal 12/16, gastric 4/16) were treated with a combination of 5-fluorouracil (5-FU) plus 1-(2-chlorethyl)-3(4-methyl-cycloexyl)-1-nitrosourea (Me-CCNU). The therapeutic program consisted of orally administered Me-CCNU (140 mg/m2) and intravenous 5-FU (9.5 mg/kg by bolus injection for 5 days). The cycles were repeated at 6-week intervals. At the beginning of the therapy, 11/16 patients were in performance status (PS) 0-1 and 5 patients in PS 2-3. Eight patients developed early progressive disease between the 1st and 2nd course of therapy. Only a minor tumor response was observed in the remaining 50% of the patients. However, the patients with stabilized disease lived longer (11.8 months) than non-responders (3.5 months).", "contents": "Combination chemotherapy with 5-fluorouracil and methyl-CCNU for the treatment of advanced gastrointestinal cancer. Sixteen patients with advanced gastrointestinal cancer (colorectal 12/16, gastric 4/16) were treated with a combination of 5-fluorouracil (5-FU) plus 1-(2-chlorethyl)-3(4-methyl-cycloexyl)-1-nitrosourea (Me-CCNU). The therapeutic program consisted of orally administered Me-CCNU (140 mg/m2) and intravenous 5-FU (9.5 mg/kg by bolus injection for 5 days). The cycles were repeated at 6-week intervals. At the beginning of the therapy, 11/16 patients were in performance status (PS) 0-1 and 5 patients in PS 2-3. Eight patients developed early progressive disease between the 1st and 2nd course of therapy. Only a minor tumor response was observed in the remaining 50% of the patients. However, the patients with stabilized disease lived longer (11.8 months) than non-responders (3.5 months)."}
{"id": "PMID:462585", "title": "Endometrial adenocarcinoma with psammoma bodies.", "content": "Psammoma bodies were found in less than 9% of 235 cases of endometrial carcinoma, considering both biopsies and surgical specimens. They may originate in papillary adenocarcinoma and do not indicate a slightly infiltrating or a well-differentiated tumor. The clinical profile of patients closely resembles that of women with endometrial carcinoma, but survival appears worse.", "contents": "Endometrial adenocarcinoma with psammoma bodies. Psammoma bodies were found in less than 9% of 235 cases of endometrial carcinoma, considering both biopsies and surgical specimens. They may originate in papillary adenocarcinoma and do not indicate a slightly infiltrating or a well-differentiated tumor. The clinical profile of patients closely resembles that of women with endometrial carcinoma, but survival appears worse."}
{"id": "PMID:462586", "title": "Angioimmunoblastic lymphadenopathy with dysproteinemia complicated by Kaposi's sarcoma.", "content": "A case of clinically and morphologically typical angioimmunoblastic lymphadenopathy (AILD) in a 68-year-old man during a prolonged antibiotic treatment for urinary infection is presented. Lymph node biopsy at first showed findings suggestive of an exhaustion of the germinal center immunological activity (like those characterizing angiofollicular lymph any clear transition into malignant lymphoma. The course of the disease was characterized by the occurrence of opportunistic infections (toxoplasmosis, herpes zoster), and finally by the onset of a cutaneous Kaposi's sarcoma. The possible relation of AILD to Kaposi's sarcoma is discussed, and the main clinical and morphological data of the case of AILD (about 200) reported in the literature are reviewed.", "contents": "Angioimmunoblastic lymphadenopathy with dysproteinemia complicated by Kaposi's sarcoma. A case of clinically and morphologically typical angioimmunoblastic lymphadenopathy (AILD) in a 68-year-old man during a prolonged antibiotic treatment for urinary infection is presented. Lymph node biopsy at first showed findings suggestive of an exhaustion of the germinal center immunological activity (like those characterizing angiofollicular lymph any clear transition into malignant lymphoma. The course of the disease was characterized by the occurrence of opportunistic infections (toxoplasmosis, herpes zoster), and finally by the onset of a cutaneous Kaposi's sarcoma. The possible relation of AILD to Kaposi's sarcoma is discussed, and the main clinical and morphological data of the case of AILD (about 200) reported in the literature are reviewed."}
{"id": "PMID:462587", "title": "A computerized cancer patient information system.", "content": "The purpose of this study was to realize a computer system for cancer data automatic processing. A clinical research in oncological practice is usually time consuming and often not reliable or deceiving because of a number of various errors. Until now, only a few information systems have been specifically designed for cancer data processing, and these have a limited capacity of autonomous data elaboration. Our system, based on an original computer program, can compile, store and process essential data on cancer diseases, with a high degree of reliability and high speed of elaboration. Input and output of this system are simplified and user oriented, without limitations for the subject at study on clinical research, and no particular training of the physicians involved is necessary.", "contents": "A computerized cancer patient information system. The purpose of this study was to realize a computer system for cancer data automatic processing. A clinical research in oncological practice is usually time consuming and often not reliable or deceiving because of a number of various errors. Until now, only a few information systems have been specifically designed for cancer data processing, and these have a limited capacity of autonomous data elaboration. Our system, based on an original computer program, can compile, store and process essential data on cancer diseases, with a high degree of reliability and high speed of elaboration. Input and output of this system are simplified and user oriented, without limitations for the subject at study on clinical research, and no particular training of the physicians involved is necessary."}
{"id": "PMID:462588", "title": "Ewing's sarcoma: an approach to radiological diagnosis.", "content": "All the pertinent radiographs of 83 patients with histologically proven Ewing's sarcoma were reviewed. Forty-nine patients were in the pediatric age group, and 34 were adults. The mean age, the symptoms and time from symptoms to diagnosis were evaluated in the 2 groups. The site of primary involvement was in 54% the long bones, 35% the flat bones, 8% the small bones and 3% extraosseous. For the primary site we considered the diagnostic results of the standard radiographic investigations and in some cases the usefulness of angiography, xeroradiography and telethermography. At presentation we also evaluated the possible diffusion of the disease with standard radiographic surveys (chest and skeletal, including limbs) and with foot lymphography in selected cases. In this way, 57 patients (69%) were considered to have localized disease. In this group, we also considered the value of the periodic radiographic follow-up, which enabled us to disclose the appearance of metastases (chest 64%, bone 54%, lymph nodes 11%) in 28 cases (49%). Finally, we made a comparison of the different radiologic and epidemiologic findings between children and adults.", "contents": "Ewing's sarcoma: an approach to radiological diagnosis. All the pertinent radiographs of 83 patients with histologically proven Ewing's sarcoma were reviewed. Forty-nine patients were in the pediatric age group, and 34 were adults. The mean age, the symptoms and time from symptoms to diagnosis were evaluated in the 2 groups. The site of primary involvement was in 54% the long bones, 35% the flat bones, 8% the small bones and 3% extraosseous. For the primary site we considered the diagnostic results of the standard radiographic investigations and in some cases the usefulness of angiography, xeroradiography and telethermography. At presentation we also evaluated the possible diffusion of the disease with standard radiographic surveys (chest and skeletal, including limbs) and with foot lymphography in selected cases. In this way, 57 patients (69%) were considered to have localized disease. In this group, we also considered the value of the periodic radiographic follow-up, which enabled us to disclose the appearance of metastases (chest 64%, bone 54%, lymph nodes 11%) in 28 cases (49%). Finally, we made a comparison of the different radiologic and epidemiologic findings between children and adults."}
{"id": "PMID:462590", "title": "[Effect of vitamin A in significant doses on incorporation of (14C) retinol, acceptor capacity of tRNA and activity of certain enzymes in tissues and plasma membranes of cells in the presence of Guerin's carcinoma].", "content": "In rats with Guerin's carcinoma the weight and size of the tumour is twice as low when significant doses of vitamin A are administered. Under these conditions the acceptor capacity of tRNA of the small intestine mucosa as well as the intensity of [14C] retinol incorporation into plasma membranes of its cells decrease essentially, whereas in the mucosa itself the radioactivity remains high. When studying the activity of alkaline phosphatase, amylase in the mucosa and arginase in the liver, a disproportion is found in changes in the enzymes activity on the surface of the small intestine mucosa and in the mucosa homogenate, that may be due to a change in the state of the cell membrane apparatus when the tumour is formed. Under the effect of significant doses of vitamin A there might occur thelysis of the tumour cell membranes, which results in the metabolism normalization.", "contents": "[Effect of vitamin A in significant doses on incorporation of (14C) retinol, acceptor capacity of tRNA and activity of certain enzymes in tissues and plasma membranes of cells in the presence of Guerin's carcinoma]. In rats with Guerin's carcinoma the weight and size of the tumour is twice as low when significant doses of vitamin A are administered. Under these conditions the acceptor capacity of tRNA of the small intestine mucosa as well as the intensity of [14C] retinol incorporation into plasma membranes of its cells decrease essentially, whereas in the mucosa itself the radioactivity remains high. When studying the activity of alkaline phosphatase, amylase in the mucosa and arginase in the liver, a disproportion is found in changes in the enzymes activity on the surface of the small intestine mucosa and in the mucosa homogenate, that may be due to a change in the state of the cell membrane apparatus when the tumour is formed. Under the effect of significant doses of vitamin A there might occur thelysis of the tumour cell membranes, which results in the metabolism normalization."}
{"id": "PMID:462591", "title": "[Metabolism of rat brain nonhistone proteins following alteration of nervous system function and training].", "content": "Metabolism of total nonhistone nuclear proteins (NNP) of the rat brain was studied in three groups of animals: trained, pseudotrained and control. Protein synthesis and degradation were estimated by determining NNP specific radioactivity 1, 3, 6 and 9 weeks after the precursor, 14C-lysine, injection. The experimental results indicate that there are two fractions of NNP which differ in their metabolic rates. The half-life of the first \"short-living\" group of proteins is 5-10 days and that of the second \"long-living\" one is 18-21 days. It is shown that there is a significant difference in specific radioactivity and metabolic rate between the \"short-living\" fractions of NNP isolated from the brains of trained or pseudotrained rats, on the one hand, and control rats, on the other hand. Possible participation of the brain total nonhistone nuclear proteins in the mechanisms of alterations of the central nervous system function and learning is discussed.", "contents": "[Metabolism of rat brain nonhistone proteins following alteration of nervous system function and training]. Metabolism of total nonhistone nuclear proteins (NNP) of the rat brain was studied in three groups of animals: trained, pseudotrained and control. Protein synthesis and degradation were estimated by determining NNP specific radioactivity 1, 3, 6 and 9 weeks after the precursor, 14C-lysine, injection. The experimental results indicate that there are two fractions of NNP which differ in their metabolic rates. The half-life of the first \"short-living\" group of proteins is 5-10 days and that of the second \"long-living\" one is 18-21 days. It is shown that there is a significant difference in specific radioactivity and metabolic rate between the \"short-living\" fractions of NNP isolated from the brains of trained or pseudotrained rats, on the one hand, and control rats, on the other hand. Possible participation of the brain total nonhistone nuclear proteins in the mechanisms of alterations of the central nervous system function and learning is discussed."}
{"id": "PMID:462589", "title": "[Effect of hydrocortisone and insulin on changes of oxidative phosphorylation after neutron irradiation].", "content": "Total double irradiation of rats with a fast neutron flow does not affect oxidative phosphorylation in the liver mitochondria but causes an essential decrease in the intensity of phosphorylation in mitochondria and chemoluminescence in blood serum of animals. Postradiation multiple administration of hydrocortisone intensifies the radiation inhibition of oxidation and phosphorylation of mitochondria and insulin administration stimulates phosphorylation in the liver mitochondria and the intensity of chemoluminescence in blood serum of rats. Hydrocortisone in combination with insulin has a less pronounced stimulating effect on phosphorylation than insulin, but the intensity of chemoluminescence in this case is higher than under the effect of each preparation separately.", "contents": "[Effect of hydrocortisone and insulin on changes of oxidative phosphorylation after neutron irradiation]. Total double irradiation of rats with a fast neutron flow does not affect oxidative phosphorylation in the liver mitochondria but causes an essential decrease in the intensity of phosphorylation in mitochondria and chemoluminescence in blood serum of animals. Postradiation multiple administration of hydrocortisone intensifies the radiation inhibition of oxidation and phosphorylation of mitochondria and insulin administration stimulates phosphorylation in the liver mitochondria and the intensity of chemoluminescence in blood serum of rats. Hydrocortisone in combination with insulin has a less pronounced stimulating effect on phosphorylation than insulin, but the intensity of chemoluminescence in this case is higher than under the effect of each preparation separately."}
{"id": "PMID:462592", "title": "[Identification of multiple molecular forms of lactate dehydrogenase during isoelectric focusing with ampholines in polyacrylamide gel thin layer].", "content": "Multiple molecular forms of lactate dehydrogenase fractionated by isoelectric focusing with ampholines in the polyacrylamide gel thin layer were identified in the supernatant fluid of the myocardium and liver homogenate of the intact rats and rabbits. Differential detection of the enzyme fractions was based on differences in their substrate dependences and sensitivity to specific inhibitors. Simultaneously the analysis was made for the partially purified preparations of lactate dehydrogenase five isoenzymes from the same sources obtained by means of the preparative electrophoresis in polyacrylamide gel and ion-exchange adsorption on DEAE-Sephadex A-50. The isoelectric points of the identified fractions are determined. It is shown that redistribution of the enzyme multiple molecular forms within the pH gradient is subordinated to a certain strictly regularity: fractions from LDH1 to LDH5 occupy successively the sites in a weak-acid, neutral and weak-alkaline regions of the pH gradient.", "contents": "[Identification of multiple molecular forms of lactate dehydrogenase during isoelectric focusing with ampholines in polyacrylamide gel thin layer]. Multiple molecular forms of lactate dehydrogenase fractionated by isoelectric focusing with ampholines in the polyacrylamide gel thin layer were identified in the supernatant fluid of the myocardium and liver homogenate of the intact rats and rabbits. Differential detection of the enzyme fractions was based on differences in their substrate dependences and sensitivity to specific inhibitors. Simultaneously the analysis was made for the partially purified preparations of lactate dehydrogenase five isoenzymes from the same sources obtained by means of the preparative electrophoresis in polyacrylamide gel and ion-exchange adsorption on DEAE-Sephadex A-50. The isoelectric points of the identified fractions are determined. It is shown that redistribution of the enzyme multiple molecular forms within the pH gradient is subordinated to a certain strictly regularity: fractions from LDH1 to LDH5 occupy successively the sites in a weak-acid, neutral and weak-alkaline regions of the pH gradient."}
{"id": "PMID:462593", "title": "[Effect of thymosine on oxidative phosphorylation in mitochondria of mouse liver in the dynamics of chemical carcinogenesis].", "content": "Oxidative phosphorylation in the mice liver mitochondria with chemical carcinogenesis was studied as affected by thymosine. It is found that under chemical carcinogenesis the energy metabolism (fraction III) lowered and administration of thymosine in the early periods of carcinogenesis favoured an increase in the indices up to the control level. In the late period of carcinogenesis the mentioned effect was not observed. The favourable effect of thymosine on the oxidative phosphorylation was associated with an increase in the immunological indices in the animals under experiment. It is supposed that the activity of thymosine as a hormonal factor includes participation in the energy metabolism regulation.", "contents": "[Effect of thymosine on oxidative phosphorylation in mitochondria of mouse liver in the dynamics of chemical carcinogenesis]. Oxidative phosphorylation in the mice liver mitochondria with chemical carcinogenesis was studied as affected by thymosine. It is found that under chemical carcinogenesis the energy metabolism (fraction III) lowered and administration of thymosine in the early periods of carcinogenesis favoured an increase in the indices up to the control level. In the late period of carcinogenesis the mentioned effect was not observed. The favourable effect of thymosine on the oxidative phosphorylation was associated with an increase in the immunological indices in the animals under experiment. It is supposed that the activity of thymosine as a hormonal factor includes participation in the energy metabolism regulation."}
{"id": "PMID:462597", "title": "[Formation of 7-dehydrocholesterol ketoderivative - cholesta-4,7-dien-3-one in stomach and intestinal rat tissues].", "content": "Incorporation of [2-14C] sodium acetate into 7-dehydrocholesterol ketoderivative, cholesta-4,7-dien-3-on, was studied in the tissues of the rat stomach secretory and esophageal parts and in the mucous and serous membranes of the small intestine. Cholesta-4,7-dien-3-on is shown to form not from [14C]cholesterol but from [14C] dehydrocholesterol. Incorporation of the label into the compound under study is established to be maximum 6h after isotope administration. The formation of cholesta-4,7-dien-3-on is the most active in the mucous membrane of small intestine and secretory part of the stomach. An assumption is advanced on the relation between the oxidative transformation of 7-dehydrocholesterol and secretion of hydrochloric acid by the animal mucous tissue.", "contents": "[Formation of 7-dehydrocholesterol ketoderivative - cholesta-4,7-dien-3-one in stomach and intestinal rat tissues]. Incorporation of [2-14C] sodium acetate into 7-dehydrocholesterol ketoderivative, cholesta-4,7-dien-3-on, was studied in the tissues of the rat stomach secretory and esophageal parts and in the mucous and serous membranes of the small intestine. Cholesta-4,7-dien-3-on is shown to form not from [14C]cholesterol but from [14C] dehydrocholesterol. Incorporation of the label into the compound under study is established to be maximum 6h after isotope administration. The formation of cholesta-4,7-dien-3-on is the most active in the mucous membrane of small intestine and secretory part of the stomach. An assumption is advanced on the relation between the oxidative transformation of 7-dehydrocholesterol and secretion of hydrochloric acid by the animal mucous tissue."}
{"id": "PMID:462598", "title": "[Application of sucrose density gradient in disk electrophoresis of proteins].", "content": "The methods are suggested when either fluid with the sucrose density gradient or the combination of sucrose with polyacrylamide gel are used as a zonal carrier in disk electrophoresis. Possibility is shown and conditions are selected for concentration and subsequent separation of proteins in the fluid with the sucrose density gradient in disk electrophoresis as narrow zones with clear boundaries. By the methods proposed the sarcoplasmic proteins of the rat myocardium and incorporated creatine kinase isoenzymes were separated.", "contents": "[Application of sucrose density gradient in disk electrophoresis of proteins]. The methods are suggested when either fluid with the sucrose density gradient or the combination of sucrose with polyacrylamide gel are used as a zonal carrier in disk electrophoresis. Possibility is shown and conditions are selected for concentration and subsequent separation of proteins in the fluid with the sucrose density gradient in disk electrophoresis as narrow zones with clear boundaries. By the methods proposed the sarcoplasmic proteins of the rat myocardium and incorporated creatine kinase isoenzymes were separated."}
{"id": "PMID:462596", "title": "[Effect of prolonged loading with lysine on distribution of free amino acids in the brain].", "content": "Prolonged loading with small amounts of lysine and a mixture of lysine and metabolism cofactors evokes considerable shifts in the distribution of the amino acid pool in the rat cerebral cortex. Under the influence of lysine the functional activity of the central nervous system improves.", "contents": "[Effect of prolonged loading with lysine on distribution of free amino acids in the brain]. Prolonged loading with small amounts of lysine and a mixture of lysine and metabolism cofactors evokes considerable shifts in the distribution of the amino acid pool in the rat cerebral cortex. Under the influence of lysine the functional activity of the central nervous system improves."}
{"id": "PMID:462594", "title": "[Effect of detergents on pancreatic ribonuclease].", "content": "It is established that the activity of pancreatic ribonuclease does not change under the effect of nonionic and some ionic detergents within a concentration range of 0.5-5.0 mg/ml and decreases in the presence of DS-Na and cetavlon (1.0 and 2.5 mg/ml, respectively). Studies on kinetics of ribonuclease thermoinactivation in the presence of different detergents showed a stabilizing effect of cetavlon and DS-Na in a concentration of 1 mg/ml.", "contents": "[Effect of detergents on pancreatic ribonuclease]. It is established that the activity of pancreatic ribonuclease does not change under the effect of nonionic and some ionic detergents within a concentration range of 0.5-5.0 mg/ml and decreases in the presence of DS-Na and cetavlon (1.0 and 2.5 mg/ml, respectively). Studies on kinetics of ribonuclease thermoinactivation in the presence of different detergents showed a stabilizing effect of cetavlon and DS-Na in a concentration of 1 mg/ml."}
{"id": "PMID:462595", "title": "[Changes in threonine and Na+ counterflow absorption in rat small intestine under the effect of insulin and F-].", "content": "The in vivo experiments showed that insulin intensifies the threonine absorption and Na+ counterflow in the rat small intestine. Fluorine inhibits considerably the threonine absorption. The possible mechanism of the insulin and fluorine opposite effects on the threonine absorption in the small intestine is under discussion.", "contents": "[Changes in threonine and Na+ counterflow absorption in rat small intestine under the effect of insulin and F-]. The in vivo experiments showed that insulin intensifies the threonine absorption and Na+ counterflow in the rat small intestine. Fluorine inhibits considerably the threonine absorption. The possible mechanism of the insulin and fluorine opposite effects on the threonine absorption in the small intestine is under discussion."}
{"id": "PMID:462648", "title": "An investigation of a spread energy method for medical ultrasound systems. Part one: theory and investigation.", "content": "A medical ultrasound device using a Golay code is proposed and investigated theoretically. The code enables the spread energy method to be used to help overcome the restraints of the parameters of resolution, peak power and penetration.", "contents": "An investigation of a spread energy method for medical ultrasound systems. Part one: theory and investigation. A medical ultrasound device using a Golay code is proposed and investigated theoretically. The code enables the spread energy method to be used to help overcome the restraints of the parameters of resolution, peak power and penetration."}
{"id": "PMID:462649", "title": "Species differences in decompression.", "content": "In an effort to bring together the diverse laboratory-animal decompression studies, a literature review and statistical evaluation were undertaken. Although 22 different species that had been used in decompression studies were identified, systematic data were available for only 7 of these species: man, goat, dog, guinea-pig, rat, hamster, and mouse. Mathematical functions using physiological data on these seven species were developed to estimate 1) saturation time (the time for the body to equilibrate after an increase in hydrostatic pressure), and 2) no-decompression saturation-exposure limits (the maximum saturation-exposure pressure from which an abrupt return to 1 ATA can be tolerated). Data from man, rat, and mouse were used to develop physiological relationships for two additional decompression variables: change in pressure-reduction limits associated with increased exposure pressure and time to onset of decompression symptoms. Finally, data on rats for two other decompression variables, gas elimination time and optimum decompression stop time, are discussed in the hope that this will stimulate additional animal laboratory research in other mammalians. The general functional relationships developed in this paper provide a preliminary and rough means for extrapolating among species the decompression results obtained during animal laboratory experiments.", "contents": "Species differences in decompression. In an effort to bring together the diverse laboratory-animal decompression studies, a literature review and statistical evaluation were undertaken. Although 22 different species that had been used in decompression studies were identified, systematic data were available for only 7 of these species: man, goat, dog, guinea-pig, rat, hamster, and mouse. Mathematical functions using physiological data on these seven species were developed to estimate 1) saturation time (the time for the body to equilibrate after an increase in hydrostatic pressure), and 2) no-decompression saturation-exposure limits (the maximum saturation-exposure pressure from which an abrupt return to 1 ATA can be tolerated). Data from man, rat, and mouse were used to develop physiological relationships for two additional decompression variables: change in pressure-reduction limits associated with increased exposure pressure and time to onset of decompression symptoms. Finally, data on rats for two other decompression variables, gas elimination time and optimum decompression stop time, are discussed in the hope that this will stimulate additional animal laboratory research in other mammalians. The general functional relationships developed in this paper provide a preliminary and rough means for extrapolating among species the decompression results obtained during animal laboratory experiments."}
{"id": "PMID:462650", "title": "Combined effects of ethanol and hyperbaric air on body sway and heart rate in man.", "content": "Eight amateur divers took part in crossover experiments to study the combined effects of ethanol (0.72 g/kg b.wt.) and hyperbaric air (4 and 6 ATA) on heart rate and body-sway movements. Body sway with open and closed eyes was measured in lateral and sagittal directions by a statometer device. In the alcohol condition, there was an initial increase in body sway corresponding to the acute phase of ethanol intoxication. At a mean blood alcohol concentration of 0.77 mg/ml, this increase in body sway was statistically significant (P less than 0.01) compared with the alcohol-free condition. At 90 min from start of drinking, body sway scores at 1 ATA were not significantly different from alcohol-free measurements. On raising the pressure to 4 and 6 ATA, increased body sway occurred in both alcohol and alcohol-free conditions and, moreover, the rate of increase was more extreme in the alcohol condition. Significant pressure-alcohol interactions were established, suggesting a potentiating action of alcohol on the increase in body sway induced by acute exposure to high pressures of air. Heart-rate measurements with and without alcohol were not significantly different, although increases in ambient pressure caused a drop in heart rate in both conditions.", "contents": "Combined effects of ethanol and hyperbaric air on body sway and heart rate in man. Eight amateur divers took part in crossover experiments to study the combined effects of ethanol (0.72 g/kg b.wt.) and hyperbaric air (4 and 6 ATA) on heart rate and body-sway movements. Body sway with open and closed eyes was measured in lateral and sagittal directions by a statometer device. In the alcohol condition, there was an initial increase in body sway corresponding to the acute phase of ethanol intoxication. At a mean blood alcohol concentration of 0.77 mg/ml, this increase in body sway was statistically significant (P less than 0.01) compared with the alcohol-free condition. At 90 min from start of drinking, body sway scores at 1 ATA were not significantly different from alcohol-free measurements. On raising the pressure to 4 and 6 ATA, increased body sway occurred in both alcohol and alcohol-free conditions and, moreover, the rate of increase was more extreme in the alcohol condition. Significant pressure-alcohol interactions were established, suggesting a potentiating action of alcohol on the increase in body sway induced by acute exposure to high pressures of air. Heart-rate measurements with and without alcohol were not significantly different, although increases in ambient pressure caused a drop in heart rate in both conditions."}
{"id": "PMID:462651", "title": "Influence of nitrous oxide, nitrogen, neon, and helium on the beating frequency of the mouse sinus node at high pressure.", "content": "The beating frequency (BF) reducing effect of 150 atm of hydrostatic pressure on mammalian cardiac pacemaker tissue (hyperbaric bradycardia) was counteracted by dissolved gas only if the gas was added after hydrostatic compression. The effect on BF seemed to be related to the narcotic potency of the gas and the effect was reversible. The gases tested were N2O, N2, Ne, and He, in decreasing order of potency. If N2O was added at a moderately raised ambient pressure prior to hydrostatic compression to 150 atm, there was no difference in the degree of hyperbaric bradycardia, compared to compression without gas. During decompression, however, experiments performed with gas showed a significantly higher gain in BF compared to experiments without gas. Autonomic blockade seemed to eliminate the difference between decompression with and without N2O. The results demonstrate that N2O, N2, and Ne, and to a small extent He, may counteract the retarding effect that increased hydrostatic pressure has on cardiac pacemaker activity. These effects on the cardiac pacemaker are similar both to the effects of increased hydrostatic pressure and of gases at elevated pressures on the central nervous system, but some important differences remain to be explained.", "contents": "Influence of nitrous oxide, nitrogen, neon, and helium on the beating frequency of the mouse sinus node at high pressure. The beating frequency (BF) reducing effect of 150 atm of hydrostatic pressure on mammalian cardiac pacemaker tissue (hyperbaric bradycardia) was counteracted by dissolved gas only if the gas was added after hydrostatic compression. The effect on BF seemed to be related to the narcotic potency of the gas and the effect was reversible. The gases tested were N2O, N2, Ne, and He, in decreasing order of potency. If N2O was added at a moderately raised ambient pressure prior to hydrostatic compression to 150 atm, there was no difference in the degree of hyperbaric bradycardia, compared to compression without gas. During decompression, however, experiments performed with gas showed a significantly higher gain in BF compared to experiments without gas. Autonomic blockade seemed to eliminate the difference between decompression with and without N2O. The results demonstrate that N2O, N2, and Ne, and to a small extent He, may counteract the retarding effect that increased hydrostatic pressure has on cardiac pacemaker activity. These effects on the cardiac pacemaker are similar both to the effects of increased hydrostatic pressure and of gases at elevated pressures on the central nervous system, but some important differences remain to be explained."}
{"id": "PMID:462652", "title": "Observations on the knee-jerk reflex in oxygen-helium at 31 and 43 bars.", "content": "Sensitivity of the knee-jerk reflex was measured in three men during two experimental saturation dives, a fast compression dive to 300 msw and a slow compression dive to 420 msw. In all cases, the force produced at the ankle by the reflex (isometric) contraction was augmented after compression. The most marked increases were observed during the shallower but faster compression dive. During decompression, reflex sensitivity remained enhanced during the 300-m dive but returned to control levels during the 420-m dive. Furthermore, the usual facilitatory effects of Jendrassik's maneuver were abolished during the 300-m dive. Peripheral nerve conduction velocity and synaptic delay apparently remained unaffected. The conclusion that the observed hyper-reflexivity results from supra-spinal influence on alpha-motor-neuron excitability and possibly on gamma-efferent activity is discussed in relation to other reflex studies in hyperbaric O2-He and O2-N2 environments.", "contents": "Observations on the knee-jerk reflex in oxygen-helium at 31 and 43 bars. Sensitivity of the knee-jerk reflex was measured in three men during two experimental saturation dives, a fast compression dive to 300 msw and a slow compression dive to 420 msw. In all cases, the force produced at the ankle by the reflex (isometric) contraction was augmented after compression. The most marked increases were observed during the shallower but faster compression dive. During decompression, reflex sensitivity remained enhanced during the 300-m dive but returned to control levels during the 420-m dive. Furthermore, the usual facilitatory effects of Jendrassik's maneuver were abolished during the 300-m dive. Peripheral nerve conduction velocity and synaptic delay apparently remained unaffected. The conclusion that the observed hyper-reflexivity results from supra-spinal influence on alpha-motor-neuron excitability and possibly on gamma-efferent activity is discussed in relation to other reflex studies in hyperbaric O2-He and O2-N2 environments."}
{"id": "PMID:462653", "title": "Effect of interaction of volatile anesthetics and high hydrostatic pressure on central neurons.", "content": "Autoactive neurons in the central nervous system of Helix and Aplysia were studied after exposure to several volatile anesthetics and under compression by mineral oil. Voltage clamp studies reveal that halothane will eliminate the slow inward current that underlies oscillatory activity in burster neurons, while high pressure shifts the negative resistance region of the current without causing its elimination. Simultaneous application of the anesthetic and pressure results in the loss of inward current over a time course similar to that of halothane application alone. It is concluded that in this system, pressure and anesthetics are not acting antagonistically at the site in the membrane that controls slow wave conductances.", "contents": "Effect of interaction of volatile anesthetics and high hydrostatic pressure on central neurons. Autoactive neurons in the central nervous system of Helix and Aplysia were studied after exposure to several volatile anesthetics and under compression by mineral oil. Voltage clamp studies reveal that halothane will eliminate the slow inward current that underlies oscillatory activity in burster neurons, while high pressure shifts the negative resistance region of the current without causing its elimination. Simultaneous application of the anesthetic and pressure results in the loss of inward current over a time course similar to that of halothane application alone. It is concluded that in this system, pressure and anesthetics are not acting antagonistically at the site in the membrane that controls slow wave conductances."}
{"id": "PMID:462654", "title": "Bubble production in agarose gels subjected to different decompression schedules.", "content": "The relative effectiveness of seven different (military, commercial, and experimental) decompression schedules in reducing bubble formation within aqueous gels has been evaluated quantitatively under rigorously controlled conditions. Specifically, visual counts have been conducted of the bubbles formed in highly purified agarose gels subjected to the different decompression schedules. The order of effectiveness among these schedules in reducing bubble formation in the agarose gel samples was as follows: Model 1 greater than Royal Naval Physiological Laboratory approximately French Ministry of Labor greater than Yount et al. greater than Japanese Department of Labor greater than United States Navy greater than French Navy. It was concluded that the depth at which slow decompression commences is a major factor, along with the total decompression time, in determining the extent of bubble formation.", "contents": "Bubble production in agarose gels subjected to different decompression schedules. The relative effectiveness of seven different (military, commercial, and experimental) decompression schedules in reducing bubble formation within aqueous gels has been evaluated quantitatively under rigorously controlled conditions. Specifically, visual counts have been conducted of the bubbles formed in highly purified agarose gels subjected to the different decompression schedules. The order of effectiveness among these schedules in reducing bubble formation in the agarose gel samples was as follows: Model 1 greater than Royal Naval Physiological Laboratory approximately French Ministry of Labor greater than Yount et al. greater than Japanese Department of Labor greater than United States Navy greater than French Navy. It was concluded that the depth at which slow decompression commences is a major factor, along with the total decompression time, in determining the extent of bubble formation."}
{"id": "PMID:462655", "title": "Correlation between decompression sickness and circulating bubbles in 232 divers.", "content": "Doppler monitoring examinations were carried out during 67 simulated helium-oxygen dives in the pressure chambers of the Centre d'Etudes Hyperbares (CEH) COMEX Marseille, and involved a total of 232 COMEX professional divers. Three to five detections were done in each 24-h period, each consisting of an observation at rest and an observation after deep knee bends. Recordings of the Doppler signals were subsequently analyzed by experienced listeners and graded according to the system described by Spencer and Johanson (1974). The two vestibular decompression accidents in this series were associated with bubble scores of grade 3 at rest; one occurred during the rapid initial phase of a bounce dive decompression and the other after return to the storage depth after an excursion dive. Twenty-five cases of muscular or joint pains were observed. A higher incidence of this type of problem was found with higher bubble grades in general, although it was not possible to predict pain.", "contents": "Correlation between decompression sickness and circulating bubbles in 232 divers. Doppler monitoring examinations were carried out during 67 simulated helium-oxygen dives in the pressure chambers of the Centre d'Etudes Hyperbares (CEH) COMEX Marseille, and involved a total of 232 COMEX professional divers. Three to five detections were done in each 24-h period, each consisting of an observation at rest and an observation after deep knee bends. Recordings of the Doppler signals were subsequently analyzed by experienced listeners and graded according to the system described by Spencer and Johanson (1974). The two vestibular decompression accidents in this series were associated with bubble scores of grade 3 at rest; one occurred during the rapid initial phase of a bounce dive decompression and the other after return to the storage depth after an excursion dive. Twenty-five cases of muscular or joint pains were observed. A higher incidence of this type of problem was found with higher bubble grades in general, although it was not possible to predict pain."}
{"id": "PMID:462677", "title": "Radionuclide evaluation of renal function.", "content": "The renal scintillation camera study and the excretory urogram should be considered to be complementary studies. The renal scintillation camera study provides an accurate evaluation of changes in total, differential, and segmental renal function but affords only a gross assessment of anatomic changes. The excretory urogram provides superior information about renal anatomic changes but only inferior information about functional changes of the kidney. The advantages of a renal scintillation camera study with regard to the patient are that it is done in a state of normal hydration, it requires no bowel preparation, it is not associated with allergic reactions, it provides a low radiation exposure, and it is a noninvasive procedure for differential renal function which requires no ureteral catheters.", "contents": "Radionuclide evaluation of renal function. The renal scintillation camera study and the excretory urogram should be considered to be complementary studies. The renal scintillation camera study provides an accurate evaluation of changes in total, differential, and segmental renal function but affords only a gross assessment of anatomic changes. The excretory urogram provides superior information about renal anatomic changes but only inferior information about functional changes of the kidney. The advantages of a renal scintillation camera study with regard to the patient are that it is done in a state of normal hydration, it requires no bowel preparation, it is not associated with allergic reactions, it provides a low radiation exposure, and it is a noninvasive procedure for differential renal function which requires no ureteral catheters."}
{"id": "PMID:462679", "title": "CT scanning in urology.", "content": "More efficient patient management, including a decrease in the length of hospital stay and a decrease in patient medical cost, may be achieved in many instances by use of the CT scanner. The clinical efficacy of this noninvasive imaging technique must continue to be judged by comparison with conventional uroradiologic techniques and careful cost analysis, but the CT scan is becoming an accepted means of assessment and diagnosis of urinary tract problems. As the technology continues to improve so will our ability to diagnose and define the extent of disease within the urinary tract.", "contents": "CT scanning in urology. More efficient patient management, including a decrease in the length of hospital stay and a decrease in patient medical cost, may be achieved in many instances by use of the CT scanner. The clinical efficacy of this noninvasive imaging technique must continue to be judged by comparison with conventional uroradiologic techniques and careful cost analysis, but the CT scan is becoming an accepted means of assessment and diagnosis of urinary tract problems. As the technology continues to improve so will our ability to diagnose and define the extent of disease within the urinary tract."}
{"id": "PMID:462683", "title": "Torsion of the testis: techniques of assessment.", "content": "Results of treatment of torsion of the testicle can be measured only by determining the rate of testicular salvage, which has been low but has improved in recent years. The Doppler instruments and the technique of scrotal imaging with the gamma camera provide noninvasive and rapid diagnostic aids with a high degree of accuracy. There is evidence that these techniques will aid in the differential diagnosis of equivocal cases, and thus lower the incidence of delay in the diagnosis of torsion, a cause of lower than optimal salvage rates in the past. Fluorescein staining can also be used in the operating room if there is a question of testicular viability. The availability of these new modalities, however, is no substitute for early evaluation. Quick referral from primary physicians and a high awareness of torsion as a cause of acute scrotal pain and swelling are extremely important. Reviews clearly indicate that improved salvage rates are achieved with early evaluation and treatment. Therefore the urologist has a continuing responsibility to educate pediatricians, family practitioners, and emergency room physicians in the urgency of diagnosis and treatment in cases of testicular torsion. Although  new diagnostic aids are useful, only early surgical exploration can save a torsive testis.", "contents": "Torsion of the testis: techniques of assessment. Results of treatment of torsion of the testicle can be measured only by determining the rate of testicular salvage, which has been low but has improved in recent years. The Doppler instruments and the technique of scrotal imaging with the gamma camera provide noninvasive and rapid diagnostic aids with a high degree of accuracy. There is evidence that these techniques will aid in the differential diagnosis of equivocal cases, and thus lower the incidence of delay in the diagnosis of torsion, a cause of lower than optimal salvage rates in the past. Fluorescein staining can also be used in the operating room if there is a question of testicular viability. The availability of these new modalities, however, is no substitute for early evaluation. Quick referral from primary physicians and a high awareness of torsion as a cause of acute scrotal pain and swelling are extremely important. Reviews clearly indicate that improved salvage rates are achieved with early evaluation and treatment. Therefore the urologist has a continuing responsibility to educate pediatricians, family practitioners, and emergency room physicians in the urgency of diagnosis and treatment in cases of testicular torsion. Although  new diagnostic aids are useful, only early surgical exploration can save a torsive testis."}
{"id": "PMID:462712", "title": "Immunopathological changes in cadmium-treated rats.", "content": "Rats treated with cadmium developed an interstitial pneumonitis with intraalveolar hemorrhage. In the spleen there was marked germinal center formation containing reactive B-cells. Lesions of the kidneys resembled those found in cadmium poisoning in man, primarily a proliferative glomerulonephritis.", "contents": "Immunopathological changes in cadmium-treated rats. Rats treated with cadmium developed an interstitial pneumonitis with intraalveolar hemorrhage. In the spleen there was marked germinal center formation containing reactive B-cells. Lesions of the kidneys resembled those found in cadmium poisoning in man, primarily a proliferative glomerulonephritis."}
{"id": "PMID:462711", "title": "The pathology of nephrotoxicity of gentamicin in snakes. A model for reptilian gout.", "content": "Two gopher snakes (Pitophis melanoleucus catenifer) each were given 5 mg/kg body weight gentamicin every 72 hours (group 1); two snakes each were given 5 mg/kg/day (group 2). Doses for both groups were given over a 2-week period. After the second week, the dose for one snake in each group was increased to 50 mg/kg/day for 2 more weeks and then discontinued. Weekly renal biopsies taken from snakes in group 1 showed no abnormalities by light microscopy during and at the completion of the experiment. Snakes in group 2 had cloudy swelling of the proximal tubules at 2 and 4 weeks after the gentamicin was administered. Snakes given the high dose of gentamicin had hydropic degeneration of the proximal tubules 2 weeks after the dose was raised to 50 mg/kg/day. This progressed to tubular necrosis 1 week after the gentamicin was discontinued. These snakes (high dose) also developed visceral gout, apparently as the result of the extensive tubular necrosis. Tophi were in the pericardium, serosal membranes and parenchyma of the kidneys, liver, spleen and lungs.", "contents": "The pathology of nephrotoxicity of gentamicin in snakes. A model for reptilian gout. Two gopher snakes (Pitophis melanoleucus catenifer) each were given 5 mg/kg body weight gentamicin every 72 hours (group 1); two snakes each were given 5 mg/kg/day (group 2). Doses for both groups were given over a 2-week period. After the second week, the dose for one snake in each group was increased to 50 mg/kg/day for 2 more weeks and then discontinued. Weekly renal biopsies taken from snakes in group 1 showed no abnormalities by light microscopy during and at the completion of the experiment. Snakes in group 2 had cloudy swelling of the proximal tubules at 2 and 4 weeks after the gentamicin was administered. Snakes given the high dose of gentamicin had hydropic degeneration of the proximal tubules 2 weeks after the dose was raised to 50 mg/kg/day. This progressed to tubular necrosis 1 week after the gentamicin was discontinued. These snakes (high dose) also developed visceral gout, apparently as the result of the extensive tubular necrosis. Tophi were in the pericardium, serosal membranes and parenchyma of the kidneys, liver, spleen and lungs."}
{"id": "PMID:462717", "title": "Prognosis after surgical excision of canine melanomas.", "content": "One hundred and thirty-four dogs from which melanomas had been excised were studied until death or for at least 2 years after surgery. Seven of 49 (14%) intraoral and lip tumours and 52 of 85 (61%) skin tumours were histologically benign; in spite of this, three of seven (43%) \"benign\" oral and four of 52 (8%) \"benign\" skin lesions led to the eventual death of the host. Thirty eight of 42 (90%) dogs with a histologically malignant melanoma of the lip or oral cavity died because of the tumour but only 15 of 33 (45%) with malignant skin melanomas died. Six of 59 (10%) dogs with a tumour of mitotic index 2 or less died from the tumour 2 years after surgery compared to 19 of 26 (73%) dogs having a tumour with a mitotic index of 3 or more.", "contents": "Prognosis after surgical excision of canine melanomas. One hundred and thirty-four dogs from which melanomas had been excised were studied until death or for at least 2 years after surgery. Seven of 49 (14%) intraoral and lip tumours and 52 of 85 (61%) skin tumours were histologically benign; in spite of this, three of seven (43%) \"benign\" oral and four of 52 (8%) \"benign\" skin lesions led to the eventual death of the host. Thirty eight of 42 (90%) dogs with a histologically malignant melanoma of the lip or oral cavity died because of the tumour but only 15 of 33 (45%) with malignant skin melanomas died. Six of 59 (10%) dogs with a tumour of mitotic index 2 or less died from the tumour 2 years after surgery compared to 19 of 26 (73%) dogs having a tumour with a mitotic index of 3 or more."}
{"id": "PMID:462718", "title": "Anomalous development of the spinal cord in a calf.", "content": "A full term Friesian bull calf was born unable to stand. The dam had no signs of illness during pregnancy. In the thoracic spinal cord there was anomalous development of the central canal and a dorsally placed fusiform and longitudinal dilatation. Mild inflammatory lesions were seen in some body organs and central nervous system where they were suggestive of viral infection. Several skeletal muscles had an extended range of muscle cell cross sectional areas and some cells had numerous internal nuclei.", "contents": "Anomalous development of the spinal cord in a calf. A full term Friesian bull calf was born unable to stand. The dam had no signs of illness during pregnancy. In the thoracic spinal cord there was anomalous development of the central canal and a dorsally placed fusiform and longitudinal dilatation. Mild inflammatory lesions were seen in some body organs and central nervous system where they were suggestive of viral infection. Several skeletal muscles had an extended range of muscle cell cross sectional areas and some cells had numerous internal nuclei."}
{"id": "PMID:462719", "title": "Bovine nasal granuloma. Gross and microscopic lesions.", "content": "The anterior nasal mucosa of 21 cattle had small closely-packed polypoid nodules. These had a distribution pattern similar to that of inhaled particles. Acute inflammatory changes consisting of eosinophil infiltration, mast cell and globule leucocyte hyperplasia, and oedema were frequent in the epithelium, terminal gland ducts and adjacent lamina propria. Epithelium over nodules was metaplastic to non-keratinising stratified squamous, whilst epithelium of terminal gland ducts was metaplastic to mucus-secreting pseudostratified columnar. Focal accumulation of granulation tissue between gland ducts caused herniations of the superficial lamina propria to form the nodules.", "contents": "Bovine nasal granuloma. Gross and microscopic lesions. The anterior nasal mucosa of 21 cattle had small closely-packed polypoid nodules. These had a distribution pattern similar to that of inhaled particles. Acute inflammatory changes consisting of eosinophil infiltration, mast cell and globule leucocyte hyperplasia, and oedema were frequent in the epithelium, terminal gland ducts and adjacent lamina propria. Epithelium over nodules was metaplastic to non-keratinising stratified squamous, whilst epithelium of terminal gland ducts was metaplastic to mucus-secreting pseudostratified columnar. Focal accumulation of granulation tissue between gland ducts caused herniations of the superficial lamina propria to form the nodules."}
{"id": "PMID:462720", "title": "Juvenile acinar atrophy of the pancreas of a dog.", "content": "A dog with juvenile acinar atrophy of the pancreas had degranulation and loss of the acinar cells. Transitional features from normal to degranulation were seen. Electron microscopically, the atrophic pancreas mainly consisted of epithelial cells of duct system, islet cells and multivesicular cells.", "contents": "Juvenile acinar atrophy of the pancreas of a dog. A dog with juvenile acinar atrophy of the pancreas had degranulation and loss of the acinar cells. Transitional features from normal to degranulation were seen. Electron microscopically, the atrophic pancreas mainly consisted of epithelial cells of duct system, islet cells and multivesicular cells."}
{"id": "PMID:462721", "title": "Spontaneous cryptosporidiosis in an adult female rabbit.", "content": "An asymptomatic adult female rabbit had intestinal cryptosporidiosis. The ileum had blunted villi, a decrease in villus-crypt ratio and a mild edema in the lamina propria. Transmission electron microscopy showed the parasite to be a Cryptosporidium similar to those reported in mouse, guinea pig, lamb, calf, horse, monkey and man. This organism is referred to as Cryptosporidium cuniculus. Scanning electron microscopy on ileal mucosa showed altered intestinal microvilli in the attachment of the cryptosporidia. It is postulated that the organism was enveloped by the microvilli of the ileal epithelial cells which then fused and formed a continuous double membrane around the parasite.", "contents": "Spontaneous cryptosporidiosis in an adult female rabbit. An asymptomatic adult female rabbit had intestinal cryptosporidiosis. The ileum had blunted villi, a decrease in villus-crypt ratio and a mild edema in the lamina propria. Transmission electron microscopy showed the parasite to be a Cryptosporidium similar to those reported in mouse, guinea pig, lamb, calf, horse, monkey and man. This organism is referred to as Cryptosporidium cuniculus. Scanning electron microscopy on ileal mucosa showed altered intestinal microvilli in the attachment of the cryptosporidia. It is postulated that the organism was enveloped by the microvilli of the ileal epithelial cells which then fused and formed a continuous double membrane around the parasite."}
{"id": "PMID:462741", "title": "Baccalaureate program in toxicology.", "content": "A meaningful baccalaureate program in toxicology will help meet the critical manpower needs in this field. A strong undergraduate program in toxicology also will benefit the science of toxicology by: (1) Relieving PhD toxicologists from more routine tasks they perform: (2) informing bright young scientists, earlier in their careers, about potential careers in toxicology; and (3) developing wider public acceptance for toxicology and safety assessment.", "contents": "Baccalaureate program in toxicology. A meaningful baccalaureate program in toxicology will help meet the critical manpower needs in this field. A strong undergraduate program in toxicology also will benefit the science of toxicology by: (1) Relieving PhD toxicologists from more routine tasks they perform: (2) informing bright young scientists, earlier in their careers, about potential careers in toxicology; and (3) developing wider public acceptance for toxicology and safety assessment."}
{"id": "PMID:462788", "title": "A simple method for the purification of poplar mosaic virus and onion yellow dwarf virus by low centrifugal forces.", "content": "A simple method for the purification of poplar mosaic virus and onion yellow dwarf virus by low centrifugal forces is described. The method yields a high concentration of purified virus, avoiding virus aggregation or fragmentation.", "contents": "A simple method for the purification of poplar mosaic virus and onion yellow dwarf virus by low centrifugal forces. A simple method for the purification of poplar mosaic virus and onion yellow dwarf virus by low centrifugal forces is described. The method yields a high concentration of purified virus, avoiding virus aggregation or fragmentation."}
{"id": "PMID:462789", "title": "Prevalence of HBsAg and anti-HBs among delivering women.", "content": "A survey of 2,500 delivering women revealed a 7.6% incidence of past infection with hepatitis B virus (HBV) (HBsAg or anti-HBs). Only 5.9% of the anti-HBs-positive mothers had a possible history of parenteral exposure to hepatitis B and none of the 55 HBsAg carriers had such a history. The findings suggest that HBV is endemic and that it is probably spread in part by nonparenteral means. The high rates of HBsAg and anti-HBs point to the possible preventive value of routine screenings for this system among pregnant women.", "contents": "Prevalence of HBsAg and anti-HBs among delivering women. A survey of 2,500 delivering women revealed a 7.6% incidence of past infection with hepatitis B virus (HBV) (HBsAg or anti-HBs). Only 5.9% of the anti-HBs-positive mothers had a possible history of parenteral exposure to hepatitis B and none of the 55 HBsAg carriers had such a history. The findings suggest that HBV is endemic and that it is probably spread in part by nonparenteral means. The high rates of HBsAg and anti-HBs point to the possible preventive value of routine screenings for this system among pregnant women."}
{"id": "PMID:462790", "title": "Prevalence of mycoplasma in the genital tract of pregnant women. Preliminary data.", "content": "The prevalence of Myoplasma hominis and \"T\" strains was investigated in 175 pregnant women in the 12th--40th week of pregnancy. It was possible to isolate the \"T\" strain from 30.3%, M. hominis from 11.4% and both strains from 9.7% of the women.", "contents": "Prevalence of mycoplasma in the genital tract of pregnant women. Preliminary data. The prevalence of Myoplasma hominis and \"T\" strains was investigated in 175 pregnant women in the 12th--40th week of pregnancy. It was possible to isolate the \"T\" strain from 30.3%, M. hominis from 11.4% and both strains from 9.7% of the women."}
{"id": "PMID:462791", "title": "Specific prophylaxis of enzootic chlamydial ovine abortion.", "content": "A vaccine against chlamydial ovine abortion was prepared according to an original method and experimentally applied to more than 500,000 animals in large breeding units. The vaccine induced a considerable seroconversion (as demonstrated by complement fixation tests) and reduced by 3--5 times the damage due to chlamydioses. Vaccination of primiparous ewes, followed by 2--3 annual revaccinations, gave good results, conferring resistance on the vaccinated animals.", "contents": "Specific prophylaxis of enzootic chlamydial ovine abortion. A vaccine against chlamydial ovine abortion was prepared according to an original method and experimentally applied to more than 500,000 animals in large breeding units. The vaccine induced a considerable seroconversion (as demonstrated by complement fixation tests) and reduced by 3--5 times the damage due to chlamydioses. Vaccination of primiparous ewes, followed by 2--3 annual revaccinations, gave good results, conferring resistance on the vaccinated animals."}
{"id": "PMID:462792", "title": "Stability of antimeasles vaccine under the influence of some physical and chemical factors.", "content": "A study on the stability of lyophilized and rehydrated measles vaccines under the influence of temperature and light was undertaken. After rehydration there was a considerable decrease in the potency of the vaccine, even when stored at +4 degrees C-- +8 DEGREES C and in the dark. Out of the several drugs tested, the commercially available drug AS was found to have a stabilizing effect on measles vaccine infectivity.", "contents": "Stability of antimeasles vaccine under the influence of some physical and chemical factors. A study on the stability of lyophilized and rehydrated measles vaccines under the influence of temperature and light was undertaken. After rehydration there was a considerable decrease in the potency of the vaccine, even when stored at +4 degrees C-- +8 DEGREES C and in the dark. Out of the several drugs tested, the commercially available drug AS was found to have a stabilizing effect on measles vaccine infectivity."}
{"id": "PMID:462793", "title": "Influenza hemagglutination-inhibiting antibodies in hospitalized children. Distribution of specific activity into IgM, IgG and IgA classes.", "content": "Sera from 75 children hospitalized with a clinical diagnosis of acute respiratory virus disease were tested by hemagglutination-inhibition as regards influenza A2 and B antibody shifts. In 48 patients there was an antibody shift during the clinical evolution of the disease (against influenza A2 in 34, B-- in 11, A2 and B in 3cases). A significant proportion of sera with antibody shift associated with a decrease of serum IgG levels and with an increase of serum IgM and IgA levels.", "contents": "Influenza hemagglutination-inhibiting antibodies in hospitalized children. Distribution of specific activity into IgM, IgG and IgA classes. Sera from 75 children hospitalized with a clinical diagnosis of acute respiratory virus disease were tested by hemagglutination-inhibition as regards influenza A2 and B antibody shifts. In 48 patients there was an antibody shift during the clinical evolution of the disease (against influenza A2 in 34, B-- in 11, A2 and B in 3cases). A significant proportion of sera with antibody shift associated with a decrease of serum IgG levels and with an increase of serum IgM and IgA levels."}
{"id": "PMID:462794", "title": "Epidemiological investigations on viral hepatitis in preschool and school communities. Note I. Prevalence and incidence of viral hepatitis and the role of the community in transmission.", "content": "A transversal and longitudinal epidemiological follow up was undertaken of the viral hepatitis cases occurring in two types of children communities (kindergartens and schools). The role of organized communities in the spread of hepatitis viruses and the contribution of external factors to the maintenance of hepatitis endemicity are discussed.", "contents": "Epidemiological investigations on viral hepatitis in preschool and school communities. Note I. Prevalence and incidence of viral hepatitis and the role of the community in transmission. A transversal and longitudinal epidemiological follow up was undertaken of the viral hepatitis cases occurring in two types of children communities (kindergartens and schools). The role of organized communities in the spread of hepatitis viruses and the contribution of external factors to the maintenance of hepatitis endemicity are discussed."}
{"id": "PMID:462795", "title": "Investigations on the presence of antibodies to Tahyna virus in Romania.", "content": "Serological results are presented of the HAI reaction to Tahyna virus performed in 1,749 apparently healthy subjects from two countries of Romania where Culex pipiens mosquitoes occur frequently, in 113 febrile patients (some of which exhibited signs of interstitial pneumonia) and in 675 domestic animals (swine, cattle, sheep and goats).", "contents": "Investigations on the presence of antibodies to Tahyna virus in Romania. Serological results are presented of the HAI reaction to Tahyna virus performed in 1,749 apparently healthy subjects from two countries of Romania where Culex pipiens mosquitoes occur frequently, in 113 febrile patients (some of which exhibited signs of interstitial pneumonia) and in 675 domestic animals (swine, cattle, sheep and goats)."}
{"id": "PMID:462819", "title": "Subfractionation of CsCl-purified H-1 parvovirus on metrizamide gradients.", "content": "The different density classes of H-l parvovirus, collected within 30 hr of infection of par-asynchronous cultures, following the standard CsCl purification step, have been shown to be heterogeneous. Rebanding of the denser form (HF, p = 1.46 g/cm3) and the less dense form (LF, p = 1.42 g/cm3) of infectious virus in the nonionic density generating solute, metrizamide, showed that both HF and LF virus bands were heterogeneous in density. The infectivity banded with isotopically labeled virus protein and DNA at 1.32 g/cm3 for both HF and LF virus. Amounts of protein and DNA which varied from preparation to preparation, but which were greater from the HF virus band, were distributed throughout the rest of the gradient, but predominated in a peak at a density of 1.2 g/cm3. The protein in this peak was without hemagglutinating activity but had the molecular weights and proportions of the H-l virion proteins (VPl, VP2', and VPZ). The DNA was of the same size as H-l DNA monomers and its proportion to the protein was similar to that of the infectious peak. The DNA was susceptible to micrococcal nuclease digestion. The nature of this noninfectious viral material thus seemed to be incompletely assembled virus. Radiolabeled H-l virus collected after 72 hr of infection formed a discrete single peak in both CsCl (p = 1.42 g/cm3), and metrizamide gradients (p = 1.32 g/cm3). There was no significant amount of the 1.20 g/cm3 viral protein-DNA complex in these mature preparations.", "contents": "Subfractionation of CsCl-purified H-1 parvovirus on metrizamide gradients. The different density classes of H-l parvovirus, collected within 30 hr of infection of par-asynchronous cultures, following the standard CsCl purification step, have been shown to be heterogeneous. Rebanding of the denser form (HF, p = 1.46 g/cm3) and the less dense form (LF, p = 1.42 g/cm3) of infectious virus in the nonionic density generating solute, metrizamide, showed that both HF and LF virus bands were heterogeneous in density. The infectivity banded with isotopically labeled virus protein and DNA at 1.32 g/cm3 for both HF and LF virus. Amounts of protein and DNA which varied from preparation to preparation, but which were greater from the HF virus band, were distributed throughout the rest of the gradient, but predominated in a peak at a density of 1.2 g/cm3. The protein in this peak was without hemagglutinating activity but had the molecular weights and proportions of the H-l virion proteins (VPl, VP2', and VPZ). The DNA was of the same size as H-l DNA monomers and its proportion to the protein was similar to that of the infectious peak. The DNA was susceptible to micrococcal nuclease digestion. The nature of this noninfectious viral material thus seemed to be incompletely assembled virus. Radiolabeled H-l virus collected after 72 hr of infection formed a discrete single peak in both CsCl (p = 1.42 g/cm3), and metrizamide gradients (p = 1.32 g/cm3). There was no significant amount of the 1.20 g/cm3 viral protein-DNA complex in these mature preparations."}
{"id": "PMID:462845", "title": "[Testosterone, epitestosterone and total estrogen excretion in men with cancer of certain localizations].", "content": "To obtain more insight into the problem on the concentration of active androgens in the male organism, the diurnal elimination of testosterone, epitestosterone and estrogens in the urine of male patients with cancer of the lung, urinary bladder, larynx and prostate (321 patients), in stage II--III, was estimated. In patients with cancer of the lung and urinary bladder testosterone was found to be lowered, while total estrogens--lowered or unchanged irrespective of age, a relative hyperestrogenization being noted in them. Contrary, in cancer of the larynx and prostate the male hormones were predominating over female ones (hyperandrogenization). In all the examined patients irrespective of the tumor process localization a relative or even absolute predominance of epitestosterone over testosterone was evident.", "contents": "[Testosterone, epitestosterone and total estrogen excretion in men with cancer of certain localizations]. To obtain more insight into the problem on the concentration of active androgens in the male organism, the diurnal elimination of testosterone, epitestosterone and estrogens in the urine of male patients with cancer of the lung, urinary bladder, larynx and prostate (321 patients), in stage II--III, was estimated. In patients with cancer of the lung and urinary bladder testosterone was found to be lowered, while total estrogens--lowered or unchanged irrespective of age, a relative hyperestrogenization being noted in them. Contrary, in cancer of the larynx and prostate the male hormones were predominating over female ones (hyperandrogenization). In all the examined patients irrespective of the tumor process localization a relative or even absolute predominance of epitestosterone over testosterone was evident."}
{"id": "PMID:462846", "title": "[State of hypophyseal thyrotropic function in the complex treatment of thyroid cancer].", "content": "Radioimmune assay of the hypophyseal thyreotropic hormone (THH) in blood of 89 patients with thyroid cancer in stage II and III indicated that the THH level prior to the treatment did not differ from that in healthy individuals--3,0 +/- 0,6 ng/ml. Subtotal thyroidectomy was associated with a sharp riste of the thyreotropin concentration in blood during the immediately 7--20 days after surgery followed by its normalization in 1--2 months. Immediately after distant gamma-therapy the THH concentration did not differ from the initial value, but in later terms it was dependent on the clinical patient's state i.e. under hypothyreosis it was increased. Increased THH level was also noted in development of the cancer recurrence following the applied therapy (without hypothyreosis).", "contents": "[State of hypophyseal thyrotropic function in the complex treatment of thyroid cancer]. Radioimmune assay of the hypophyseal thyreotropic hormone (THH) in blood of 89 patients with thyroid cancer in stage II and III indicated that the THH level prior to the treatment did not differ from that in healthy individuals--3,0 +/- 0,6 ng/ml. Subtotal thyroidectomy was associated with a sharp riste of the thyreotropin concentration in blood during the immediately 7--20 days after surgery followed by its normalization in 1--2 months. Immediately after distant gamma-therapy the THH concentration did not differ from the initial value, but in later terms it was dependent on the clinical patient's state i.e. under hypothyreosis it was increased. Increased THH level was also noted in development of the cancer recurrence following the applied therapy (without hypothyreosis)."}
{"id": "PMID:462847", "title": "[Use of hormone loading tests for assessing the source of hyperestrogenism and the functional state of the ovaries in breast cancer patients in menopause].", "content": "The source of hyperestrogenia was ascertained in 12 patients by dexametasone-infecundin test, and the ovarian hormonal activity in 5 patients by dexametasone-choriogonin test. The source of hyperestrogenia was found to be the adrenal glands in 50%, ovaries--in 30% and the adrenal together with ovaries--in 20%. Ovarian hormonal activity was retained in 3 of 5 patients under examination. The response to the test concerned was changed in two of them. It is felt that application of hormonal stress tests will make it possible to get detailed indications to castration in menopausal patients with breast cancer.", "contents": "[Use of hormone loading tests for assessing the source of hyperestrogenism and the functional state of the ovaries in breast cancer patients in menopause]. The source of hyperestrogenia was ascertained in 12 patients by dexametasone-infecundin test, and the ovarian hormonal activity in 5 patients by dexametasone-choriogonin test. The source of hyperestrogenia was found to be the adrenal glands in 50%, ovaries--in 30% and the adrenal together with ovaries--in 20%. Ovarian hormonal activity was retained in 3 of 5 patients under examination. The response to the test concerned was changed in two of them. It is felt that application of hormonal stress tests will make it possible to get detailed indications to castration in menopausal patients with breast cancer."}
{"id": "PMID:462848", "title": "[Determination of estradiol-17 beta receptors in tumorous breast tissue as an index of the hormonal dependence of the tumor].", "content": "To judge the hormone-dependence of mammary tumors the technic of tissue sections was employed, which were examined for the presence of oestradiol-17 beta receptors. Under examination were 192 patients, 78 of them being in their reproductive age and 14 menopausal patients. Oestradiol-17 beta receptors were found in the tumor material from 42.7% of patients.", "contents": "[Determination of estradiol-17 beta receptors in tumorous breast tissue as an index of the hormonal dependence of the tumor]. To judge the hormone-dependence of mammary tumors the technic of tissue sections was employed, which were examined for the presence of oestradiol-17 beta receptors. Under examination were 192 patients, 78 of them being in their reproductive age and 14 menopausal patients. Oestradiol-17 beta receptors were found in the tumor material from 42.7% of patients."}
{"id": "PMID:462849", "title": "[Electron microscopic characteristics of serous and endometrioid neoplasms of the ovaries].", "content": "Using electron microscopy the authors have studied the operation material of 15 patients with serous (9 cases) and endometrioid (6 cases) ovarian neoplasms. It was demonstrated that the epithelium of endometrioid neoplasms differs from that of serous ones by less polymorphism, the absence of cilia, the signs of secretory activity and more intense development of tonofibrils in the cytoplasm.", "contents": "[Electron microscopic characteristics of serous and endometrioid neoplasms of the ovaries]. Using electron microscopy the authors have studied the operation material of 15 patients with serous (9 cases) and endometrioid (6 cases) ovarian neoplasms. It was demonstrated that the epithelium of endometrioid neoplasms differs from that of serous ones by less polymorphism, the absence of cilia, the signs of secretory activity and more intense development of tonofibrils in the cytoplasm."}
{"id": "PMID:462850", "title": "[Evaluation of the effectiveness of the radiation component in the combined treatment of cervical cancer].", "content": "The author presents an analysis of the issues of different variants of the combination treatment for cervical cancer in 551 patients. In 246 of them the therapeutic schedule included the preoperative irradiation, in 305--the postoperative one. The preoperative irradiation was conducted following the fractional-protracted and large fractional techniques. Use of the irradiation preoperatively made it feasible to increase a 5-year survival in the patients; the fractional-protracted technique, however, yielded a greater number of the postoperative complications. Irradiation conducted in the regimen of large fractionation dose allowed obviating many of these complications and retaining the advantages of the combination treatment with preoperative radiotherapy.", "contents": "[Evaluation of the effectiveness of the radiation component in the combined treatment of cervical cancer]. The author presents an analysis of the issues of different variants of the combination treatment for cervical cancer in 551 patients. In 246 of them the therapeutic schedule included the preoperative irradiation, in 305--the postoperative one. The preoperative irradiation was conducted following the fractional-protracted and large fractional techniques. Use of the irradiation preoperatively made it feasible to increase a 5-year survival in the patients; the fractional-protracted technique, however, yielded a greater number of the postoperative complications. Irradiation conducted in the regimen of large fractionation dose allowed obviating many of these complications and retaining the advantages of the combination treatment with preoperative radiotherapy."}
{"id": "PMID:462851", "title": "[Effect of hypothalamic electrostimulation on the 11-hydroxy-corticosteroid content in the blood and on the immunological indices in rabbits with Brown-Pearce carcinoma].", "content": "In animals with Brown--Pearce carcinoma electric stimulation of the hypothalamus makes it possible to model an increased corticosterone level in blood at early stages of the tumor growth, which conditions more active development of antitumor reactions and consequently an enhanced tumor resorption. The increased level of free corticosterone seems to be essential for \"triggering\" and an active mode of proceeding of antitumor immune reactions.", "contents": "[Effect of hypothalamic electrostimulation on the 11-hydroxy-corticosteroid content in the blood and on the immunological indices in rabbits with Brown-Pearce carcinoma]. In animals with Brown--Pearce carcinoma electric stimulation of the hypothalamus makes it possible to model an increased corticosterone level in blood at early stages of the tumor growth, which conditions more active development of antitumor reactions and consequently an enhanced tumor resorption. The increased level of free corticosterone seems to be essential for \"triggering\" and an active mode of proceeding of antitumor immune reactions."}
{"id": "PMID:462852", "title": "[Clonogenic cell response of murine NK Ly/LL solid tumor to N-methyl-N-nitrosourea].", "content": "By the in vitro technic for obtaining clones of cells of murine solid tumor NKLy/LL it was demonstrated that clonogenic cells survival rate versus NNU dosage curve in the interval of 12.5--200.0 mg/kg is exponential and characterized by the dosage value D0 = 29.15 mg/kg. In large size tumors (15.0--17.0 g) which higher death rate of clonogenic cells was noted (the survived fraction approximately 0.5%) than in tumors weighing 1.0--7.0 g (the survived fraction--2--4%), that seems to be related to a greater sensitivity of resting tumor cells to MNU.", "contents": "[Clonogenic cell response of murine NK Ly/LL solid tumor to N-methyl-N-nitrosourea]. By the in vitro technic for obtaining clones of cells of murine solid tumor NKLy/LL it was demonstrated that clonogenic cells survival rate versus NNU dosage curve in the interval of 12.5--200.0 mg/kg is exponential and characterized by the dosage value D0 = 29.15 mg/kg. In large size tumors (15.0--17.0 g) which higher death rate of clonogenic cells was noted (the survived fraction approximately 0.5%) than in tumors weighing 1.0--7.0 g (the survived fraction--2--4%), that seems to be related to a greater sensitivity of resting tumor cells to MNU."}
{"id": "PMID:462853", "title": "[Development of a transplantable mammary gland cancer in rats at different thyroid hormone levels].", "content": "In experiments on female rats of various age the authors studied the effect of thyroid hormones on the growth of the mammary gland transplantable cancer. In young animals the percentage of spontaneous tumor regression and the survival were found to increase with age, synchronously with a rise in blood thyroxine level. Injection of thyroxine in a dose of 5 mkg into 2-month old animals induces marked inhibition of the growth, and in later terms a complete tumor regression in 78% of cases. In thyroidectomized rats a rapid tumor growth was terminated by death in all animals of this series. It is the authors' opinion that thyroid hormones stimulate the transplantation immunity, correct the functioning of the hypothalamo-hypophyseal-genital system.", "contents": "[Development of a transplantable mammary gland cancer in rats at different thyroid hormone levels]. In experiments on female rats of various age the authors studied the effect of thyroid hormones on the growth of the mammary gland transplantable cancer. In young animals the percentage of spontaneous tumor regression and the survival were found to increase with age, synchronously with a rise in blood thyroxine level. Injection of thyroxine in a dose of 5 mkg into 2-month old animals induces marked inhibition of the growth, and in later terms a complete tumor regression in 78% of cases. In thyroidectomized rats a rapid tumor growth was terminated by death in all animals of this series. It is the authors' opinion that thyroid hormones stimulate the transplantation immunity, correct the functioning of the hypothalamo-hypophyseal-genital system."}
{"id": "PMID:462860", "title": "[Experimental data to substantiate the antitumor action of hydrazine sulfate].", "content": "It was found that under the influence of hydrazine sulfate, which in the dosage of 60 mg/kg would induce a 50% suppression of Walker carcinosarcoma in rats, there is the decreased activity of mitochondrial monoamine oxidase and a sharp reduction in the activity of some microsomal phosphatases. Vitamin B6 injection some hours before injecting hydrazine sulfate lessens the rate of suppression of the tumor growth up to 50%. The results obtained indicate that the antitumor effect of hydrazine sulfate may be related to the suppressed monoamine oxidate activity and vitamin B6 deficiency.", "contents": "[Experimental data to substantiate the antitumor action of hydrazine sulfate]. It was found that under the influence of hydrazine sulfate, which in the dosage of 60 mg/kg would induce a 50% suppression of Walker carcinosarcoma in rats, there is the decreased activity of mitochondrial monoamine oxidase and a sharp reduction in the activity of some microsomal phosphatases. Vitamin B6 injection some hours before injecting hydrazine sulfate lessens the rate of suppression of the tumor growth up to 50%. The results obtained indicate that the antitumor effect of hydrazine sulfate may be related to the suppressed monoamine oxidate activity and vitamin B6 deficiency."}
{"id": "PMID:462861", "title": "[Tropism of radiopharmacologic preparations in lymphogramulomatosis in mice].", "content": "In experiments on 75 female mice of line SJL/J with lymphograulomatosis, 75 female mice of the same line without lymphogranulomatosis and intact non-lineal mice, the authors have studied the labelled tumorigenic compounds: indium-111, gallium-67-citrates and sodium selenium-75-selenite. It was found that only sodium selenium-75-selenite possesses an enhanced ability to be accumulated in lymphogranulomatosis involved lymph nodes in mice. Whereas indium-111 and gallium-67-citrates contrary to the literature data available showed no enhanced tropism to lymph nodes of mice with lymphogranulomatosis.", "contents": "[Tropism of radiopharmacologic preparations in lymphogramulomatosis in mice]. In experiments on 75 female mice of line SJL/J with lymphograulomatosis, 75 female mice of the same line without lymphogranulomatosis and intact non-lineal mice, the authors have studied the labelled tumorigenic compounds: indium-111, gallium-67-citrates and sodium selenium-75-selenite. It was found that only sodium selenium-75-selenite possesses an enhanced ability to be accumulated in lymphogranulomatosis involved lymph nodes in mice. Whereas indium-111 and gallium-67-citrates contrary to the literature data available showed no enhanced tropism to lymph nodes of mice with lymphogranulomatosis."}
{"id": "PMID:462862", "title": "[Cancer of the organs of the pancreatoduodenal region (based on autopsy data from 2 hospitals in the city of Donetsk)].", "content": "The incidence of cancer of the pancreatoduodenal organs (the pancreas, major duodenal papilla, extrahepatic bile ducts, duodenum) is reported according to the autopsy findings obtained at two clinical hospitals (provincial and municipal) of Donetsk city during the period from 1957 to 1973. There is a tendency to the increased mortality due to cancer of the localization concerned against the background of the reduced total mortality.", "contents": "[Cancer of the organs of the pancreatoduodenal region (based on autopsy data from 2 hospitals in the city of Donetsk)]. The incidence of cancer of the pancreatoduodenal organs (the pancreas, major duodenal papilla, extrahepatic bile ducts, duodenum) is reported according to the autopsy findings obtained at two clinical hospitals (provincial and municipal) of Donetsk city during the period from 1957 to 1973. There is a tendency to the increased mortality due to cancer of the localization concerned against the background of the reduced total mortality."}
{"id": "PMID:462863", "title": "[Liver function in lymphogranulomatosis].", "content": "In 71 patients with lymphogranulomatosis (35 males and 36 females aged from 19 to 67 years) the hepatic function was studied prior to and during the cytostatic treatment. There were found considerable disturbances in the liver functioning and parallelism between the activity of the mentioned enzymes and the presence of intoxication, the enlarged liver size and intensity of the pathological process. During the treatment in some patients the activity of the enzymes under study was decreased or normalized. Changes in the activity of blood transminase, aldolase and alkaline phosphatase would reflect the gravity of destructive processes and disturbances in the liver function in patients with lymphogranulomatosis. The data obtained are of certain value for the treatment and prognosis of the disease.", "contents": "[Liver function in lymphogranulomatosis]. In 71 patients with lymphogranulomatosis (35 males and 36 females aged from 19 to 67 years) the hepatic function was studied prior to and during the cytostatic treatment. There were found considerable disturbances in the liver functioning and parallelism between the activity of the mentioned enzymes and the presence of intoxication, the enlarged liver size and intensity of the pathological process. During the treatment in some patients the activity of the enzymes under study was decreased or normalized. Changes in the activity of blood transminase, aldolase and alkaline phosphatase would reflect the gravity of destructive processes and disturbances in the liver function in patients with lymphogranulomatosis. The data obtained are of certain value for the treatment and prognosis of the disease."}
{"id": "PMID:462866", "title": "[Differential cytological diagnosis of lymph node lesions].", "content": "In cytological assay of the material, obtained from 144 patients with different lymph node lesions, the precise diagnosis was established in 119 observations (82.6%), the diagnosis remained unestablished or was erroneous in 25 cases (17.4%). It was found that of primary importance in establishing the differential cytological diagnosis of lymph node lesions are cytomorphological criteria, the role of cytochemical studies is only axilliary.", "contents": "[Differential cytological diagnosis of lymph node lesions]. In cytological assay of the material, obtained from 144 patients with different lymph node lesions, the precise diagnosis was established in 119 observations (82.6%), the diagnosis remained unestablished or was erroneous in 25 cases (17.4%). It was found that of primary importance in establishing the differential cytological diagnosis of lymph node lesions are cytomorphological criteria, the role of cytochemical studies is only axilliary."}
{"id": "PMID:462867", "title": "[Supression of the lymphocyte blast transformation reaction evoked in human subjects by a fat load].", "content": "It has been shown that \"Intralipid\"--triglycerides emulsion, used for parenteral feeding, administered intravenously in the amount of 200 ml would induce within 2 hours in human subjects a metabolic immunosuppression, estimated by a drop in the labelled thymidine and uridine incorporation into phytohemagglutinin stimulated lymphocytes. The dosage blood loss (25 ml of blood) would prevent the inhibition of lymphocytic blasttransformation reaction, that seems to prove the functional nature of metabolic immunosuppression, induced by the increased concentration of fatty acids in blood due to hydrolysis of administered triglycerides. The relationship between metabolic immunosuppression and the standard methods of treatment used in oncology is briefly discussed.", "contents": "[Supression of the lymphocyte blast transformation reaction evoked in human subjects by a fat load]. It has been shown that \"Intralipid\"--triglycerides emulsion, used for parenteral feeding, administered intravenously in the amount of 200 ml would induce within 2 hours in human subjects a metabolic immunosuppression, estimated by a drop in the labelled thymidine and uridine incorporation into phytohemagglutinin stimulated lymphocytes. The dosage blood loss (25 ml of blood) would prevent the inhibition of lymphocytic blasttransformation reaction, that seems to prove the functional nature of metabolic immunosuppression, induced by the increased concentration of fatty acids in blood due to hydrolysis of administered triglycerides. The relationship between metabolic immunosuppression and the standard methods of treatment used in oncology is briefly discussed."}
{"id": "PMID:462868", "title": "[Morphology and the morphogenesis aspects of ovarian cystomas].", "content": "Use of the complex of morphological methods (histological, morphometric, electron microscopy and tissue culture technic) in examining the operation material enabled a more precise and full idea to be formulated of the principal stages of the morphogenesis of serous ovarian cystomas and also allowed the validation of their differentiation between benign, boundary (intermediate) and malignant types.", "contents": "[Morphology and the morphogenesis aspects of ovarian cystomas]. Use of the complex of morphological methods (histological, morphometric, electron microscopy and tissue culture technic) in examining the operation material enabled a more precise and full idea to be formulated of the principal stages of the morphogenesis of serous ovarian cystomas and also allowed the validation of their differentiation between benign, boundary (intermediate) and malignant types."}
{"id": "PMID:462869", "title": "[3-hydroxyanthranilic acid content in the urine of bladder cancer patients].", "content": "The work has shown the possibility to determine immunologically 3-OAA-antigen in the urine of bladder cancer patients, which is absent in the urine of healthy individuals. By the identity reaction the authors proved the supposed structure of the detected antigen, i. e. the presence of 3-oxianthranilic acid in it. The data obtained may lie in the basis of the immunodiagnostic test to bladder cancer.", "contents": "[3-hydroxyanthranilic acid content in the urine of bladder cancer patients]. The work has shown the possibility to determine immunologically 3-OAA-antigen in the urine of bladder cancer patients, which is absent in the urine of healthy individuals. By the identity reaction the authors proved the supposed structure of the detected antigen, i. e. the presence of 3-oxianthranilic acid in it. The data obtained may lie in the basis of the immunodiagnostic test to bladder cancer."}
{"id": "PMID:462870", "title": "[Morphometric analysis of the ultrastructure of tumor cells with a varying protein synthesis specificity].", "content": "The author has studied morphometrically the cell ultrastructure of fibroblastic and smooth muscle human tumors. There were found some differences in the quantitative features of the energetic (mitochondria) and protein-synthesizing (ergastoplasm and polyribosomes) cellular apparatus of these neoplasms. The specific criteria of the malignancy grade were identified for both groups of tumors.", "contents": "[Morphometric analysis of the ultrastructure of tumor cells with a varying protein synthesis specificity]. The author has studied morphometrically the cell ultrastructure of fibroblastic and smooth muscle human tumors. There were found some differences in the quantitative features of the energetic (mitochondria) and protein-synthesizing (ergastoplasm and polyribosomes) cellular apparatus of these neoplasms. The specific criteria of the malignancy grade were identified for both groups of tumors."}
{"id": "PMID:462871", "title": "[Evaluation of diagnostic methods in kidney cancer].", "content": "Different methods for the renal cancer diagnosis: roentgenological, vasographic, radioisotopic, cytological, immunological and others are assessed comparatively. Among the routine roentgenological methods excretory and infusion urography, retropneumoperitoneum associated with tomography and excretory urography, retrograde pyelography proved to be effective. Kidney scanning with neohydrine is a simple and safe technic of examination. Selective arteriography is the most effective method for recognizing renal cancer, however in 7.2% of patients the response was negative. Cytological and immunological methods are of a secondary value. The scheme of examining patients suspected of renal cancer is recommended.", "contents": "[Evaluation of diagnostic methods in kidney cancer]. Different methods for the renal cancer diagnosis: roentgenological, vasographic, radioisotopic, cytological, immunological and others are assessed comparatively. Among the routine roentgenological methods excretory and infusion urography, retropneumoperitoneum associated with tomography and excretory urography, retrograde pyelography proved to be effective. Kidney scanning with neohydrine is a simple and safe technic of examination. Selective arteriography is the most effective method for recognizing renal cancer, however in 7.2% of patients the response was negative. Cytological and immunological methods are of a secondary value. The scheme of examining patients suspected of renal cancer is recommended."}
{"id": "PMID:462872", "title": "[Prognostic significance of the morphological signs of the malignancy of a pheochromocytoma].", "content": "The authors have studied the prognostic value of such convincing morphological signs of tumor malignancy as cellular and nuclear polymorphism, the mitotic activity, the invasion of tumor cells into the vascular lumen, the presence of metastases in 28 operated patients with a hormone-active tumor of chromaffin tissue. The results of late-term observations within the period from 2 to 17 years indicated that all the patients subjected to radical surgery are alive. Detecting in a histological assay of pheochromocytoma of the aforementioned signs of malignancy except metastases is not an obligatory prognostically unfavourable factor. Such tumor cannot be called pheochromoblastoma, but it should be defined as pheochromocytoma with the morphological signs of malignancy.", "contents": "[Prognostic significance of the morphological signs of the malignancy of a pheochromocytoma]. The authors have studied the prognostic value of such convincing morphological signs of tumor malignancy as cellular and nuclear polymorphism, the mitotic activity, the invasion of tumor cells into the vascular lumen, the presence of metastases in 28 operated patients with a hormone-active tumor of chromaffin tissue. The results of late-term observations within the period from 2 to 17 years indicated that all the patients subjected to radical surgery are alive. Detecting in a histological assay of pheochromocytoma of the aforementioned signs of malignancy except metastases is not an obligatory prognostically unfavourable factor. Such tumor cannot be called pheochromoblastoma, but it should be defined as pheochromocytoma with the morphological signs of malignancy."}
{"id": "PMID:462873", "title": "[Effect of diethylnitrosamine on the protein-synthesizing cell system of the rat liver].", "content": "Diethylnitrosoamine is found to alter the course of the regenerative processes induced by partial hepatectomy, which is manifested in essentially other, than in the control, changes of polyribosomes and mRNA. In early terms following DENA injection one may observe a reduced content of total polyribosomes, a sharp decrease in the amount of membrane-bound polyribosomes, the inhibited incorporation of newly produced mRNA in both classes of polyribosomes. In later terms, a proportion of the membrane-bound polyribosomes is again diminished, while the ratio of the specific radioactivity of free and membrane-bound polyribosomes is raised.", "contents": "[Effect of diethylnitrosamine on the protein-synthesizing cell system of the rat liver]. Diethylnitrosoamine is found to alter the course of the regenerative processes induced by partial hepatectomy, which is manifested in essentially other, than in the control, changes of polyribosomes and mRNA. In early terms following DENA injection one may observe a reduced content of total polyribosomes, a sharp decrease in the amount of membrane-bound polyribosomes, the inhibited incorporation of newly produced mRNA in both classes of polyribosomes. In later terms, a proportion of the membrane-bound polyribosomes is again diminished, while the ratio of the specific radioactivity of free and membrane-bound polyribosomes is raised."}
{"id": "PMID:462874", "title": "[Prognostic significance of sinus histocytosis of the regional lymph nodes in stomach cancer].", "content": "A comparative study was made on different morphological pictures of the sinuses of the regional lymph nodes in gastric cancer patients with T1--4N0M0 with a 5-year survival, also the influence of age, depth of invasion, size and histological structure of the tumor on sinus histiocytosis was studied. Marked sinus histiocytosis, contrary to sinus fibrosis, was found to be a favourable prognostic sign. Sinus histiocytosis is influenced in a somewhat directly proportional way by the tumor size and histological differentiation. The given material rendered impossible to reveal any effect of age and depth of tumor invasion on sinus histiocytosis.", "contents": "[Prognostic significance of sinus histocytosis of the regional lymph nodes in stomach cancer]. A comparative study was made on different morphological pictures of the sinuses of the regional lymph nodes in gastric cancer patients with T1--4N0M0 with a 5-year survival, also the influence of age, depth of invasion, size and histological structure of the tumor on sinus histiocytosis was studied. Marked sinus histiocytosis, contrary to sinus fibrosis, was found to be a favourable prognostic sign. Sinus histiocytosis is influenced in a somewhat directly proportional way by the tumor size and histological differentiation. The given material rendered impossible to reveal any effect of age and depth of tumor invasion on sinus histiocytosis."}
{"id": "PMID:462875", "title": "[Characteristics of the action of N-nitrosoethylurea in rat embryogenesis].", "content": "Based on the experiments with single intravenous injections of NEU in rats with definite pregnancy terms, it was found the embryos are especially sensitive to embryotoxic effects at the 3d--4th and 9th day, to teratogenic effects--at the 9th--14th day. The carcinogenic effect of NMU (20 mg/kg) is evident following the exposure, starting from the 11th day of embryogenesis, and the former is equally maximum during the subsequent terms until the end of the intrauterine period, their offsprings developing multiple tumors of the central and peripheral nervous systems. The sensitivity of rat embryos to NMU carcinogenic effect, contrary to the embryotoxic and teratogenic ones, showed no strict staging character. No correlation was noted between gross developmental defects and the appearance of tumors.", "contents": "[Characteristics of the action of N-nitrosoethylurea in rat embryogenesis]. Based on the experiments with single intravenous injections of NEU in rats with definite pregnancy terms, it was found the embryos are especially sensitive to embryotoxic effects at the 3d--4th and 9th day, to teratogenic effects--at the 9th--14th day. The carcinogenic effect of NMU (20 mg/kg) is evident following the exposure, starting from the 11th day of embryogenesis, and the former is equally maximum during the subsequent terms until the end of the intrauterine period, their offsprings developing multiple tumors of the central and peripheral nervous systems. The sensitivity of rat embryos to NMU carcinogenic effect, contrary to the embryotoxic and teratogenic ones, showed no strict staging character. No correlation was noted between gross developmental defects and the appearance of tumors."}
{"id": "PMID:462876", "title": "[Dynamics of the connective tissue changes in rats in the process of tumor formation induced by ethacryl (AKR-15) plastic].", "content": "Morphological studies of the rat connective tissue around the implanted plates, made from a plastic Ethacryl (AKP-15) being employed in orthopedic stomatology, have demonstrated a gradual reduction of the proliferative activity of the connective tissue capsule fibroblasts, followed 6 months later by the appearance of diffuse and then focal proliferates in the internal layer with subsequent development of tumors. A histological assay of the collagen-formation revealed no dystrophic changes in the connective tissue characteristic of other types of blastomogenesis. The pattern of diformazane depositing, when histochemically assaying oxido-reduction enzymes, may evidence the cell damage of the connective tissue capsules around the implant in the first months of the experiment.", "contents": "[Dynamics of the connective tissue changes in rats in the process of tumor formation induced by ethacryl (AKR-15) plastic]. Morphological studies of the rat connective tissue around the implanted plates, made from a plastic Ethacryl (AKP-15) being employed in orthopedic stomatology, have demonstrated a gradual reduction of the proliferative activity of the connective tissue capsule fibroblasts, followed 6 months later by the appearance of diffuse and then focal proliferates in the internal layer with subsequent development of tumors. A histological assay of the collagen-formation revealed no dystrophic changes in the connective tissue characteristic of other types of blastomogenesis. The pattern of diformazane depositing, when histochemically assaying oxido-reduction enzymes, may evidence the cell damage of the connective tissue capsules around the implant in the first months of the experiment."}
{"id": "PMID:462877", "title": "[Blastomogenic activity of 2-aminoacetophenone in Syrian hamsters].", "content": "The blastomogenic activity of 2-aminoacetophenone (2-AAP)--one of the aromatic triptophan derivatives, found in the urine of leukemic patients, was studied on nonlineal Syrian hamsters, 2-AAP would induce the increased total incidence of tumors and raise the frequency of endocrine system tumors, especially adrenal adenomas. The results of the experiment indicate an insignificant blastomogenic activity of 2-AAP and correlate well with the results obtained on lineal mice.", "contents": "[Blastomogenic activity of 2-aminoacetophenone in Syrian hamsters]. The blastomogenic activity of 2-aminoacetophenone (2-AAP)--one of the aromatic triptophan derivatives, found in the urine of leukemic patients, was studied on nonlineal Syrian hamsters, 2-AAP would induce the increased total incidence of tumors and raise the frequency of endocrine system tumors, especially adrenal adenomas. The results of the experiment indicate an insignificant blastomogenic activity of 2-AAP and correlate well with the results obtained on lineal mice."}
{"id": "PMID:462878", "title": "[Ultrastructural changes in the liver of rats with a Yoshida tumor under exposure to cyclophosphamide].", "content": "Sixteen Wistar line rats with Joshida tumor were given cyclophosphamide either daily or with two-day intervals. The changes observed were mostly those related with the Golgi complex apparatus, lysosomes, biliary canaliculi as well as hyaloplasma. The degree of pronouncement of these changes was dependent on the duration of cyclophosphamide administration. The ultrastructure of biliary canaliculi changed according to the type, observed in intrahepatic cholestasis, makes it mandatory to be careful while using cyclophosphamide in patients with the cholestatic syndrome.", "contents": "[Ultrastructural changes in the liver of rats with a Yoshida tumor under exposure to cyclophosphamide]. Sixteen Wistar line rats with Joshida tumor were given cyclophosphamide either daily or with two-day intervals. The changes observed were mostly those related with the Golgi complex apparatus, lysosomes, biliary canaliculi as well as hyaloplasma. The degree of pronouncement of these changes was dependent on the duration of cyclophosphamide administration. The ultrastructure of biliary canaliculi changed according to the type, observed in intrahepatic cholestasis, makes it mandatory to be careful while using cyclophosphamide in patients with the cholestatic syndrome."}
{"id": "PMID:462879", "title": "[Factor analysis of the effectiveness of combined therapy in experimental leukemia].", "content": "The effectiveness of the combined therapy of leukemia P-388 with cyclophosphane and diazane was studied. The pattern of administering these substances was selected with due consideration of the cyclospecificity of their action. In a number of cases considerable synergism was observed, resulting in a cure of 100% of animals. Estimation of the effectiveness and the degree of synergism was made by the method of fractionation analysis, which allowed delineating some trends for providing the optimum combined therapy.", "contents": "[Factor analysis of the effectiveness of combined therapy in experimental leukemia]. The effectiveness of the combined therapy of leukemia P-388 with cyclophosphane and diazane was studied. The pattern of administering these substances was selected with due consideration of the cyclospecificity of their action. In a number of cases considerable synergism was observed, resulting in a cure of 100% of animals. Estimation of the effectiveness and the degree of synergism was made by the method of fractionation analysis, which allowed delineating some trends for providing the optimum combined therapy."}
{"id": "PMID:462886", "title": "[Diet therapy in renal insufficiency].", "content": "In renal insufficiency the low-protein diet should be considered of reduction and temporary type. After clinical improvement it is advisable to place the patients on diet with a higher protein content and in this way to avert any further fall in the concentration of essential amino acids in the plasma and thus keep down the progressive protein-caloric fasting. The disturbance of the protein metabolism is, however, provoked not only by the protein-caloric deprivation, but by the disease itself. In this way, the developing upsets distort the picture of the protein-calorie deficiency, which finds its expression in the appearance of the anemic syndrome, disturbed electrolytes metabolism, imbalance of the acid-base equilibrium, etc. Therefore, one should look for other ways for their compensation, not believing that this can be achieved through the dietotherapy alone.", "contents": "[Diet therapy in renal insufficiency]. In renal insufficiency the low-protein diet should be considered of reduction and temporary type. After clinical improvement it is advisable to place the patients on diet with a higher protein content and in this way to avert any further fall in the concentration of essential amino acids in the plasma and thus keep down the progressive protein-caloric fasting. The disturbance of the protein metabolism is, however, provoked not only by the protein-caloric deprivation, but by the disease itself. In this way, the developing upsets distort the picture of the protein-calorie deficiency, which finds its expression in the appearance of the anemic syndrome, disturbed electrolytes metabolism, imbalance of the acid-base equilibrium, etc. Therefore, one should look for other ways for their compensation, not believing that this can be achieved through the dietotherapy alone."}
{"id": "PMID:462888", "title": "[Natural feeding and its importance in forming local immunological protection in the gastrointestinal tract during the neonatal period].", "content": "The immune proteins content in the breast milk at the 1st week of lactation as contrasted to the data obtained by investigating immunoglobulins in feces of newborns breast-fed was studied. Both in the breast milk and in feces the A-class immunoglobulins being of great importance in the immunological protection of the mucosae prevail. A high level of immune proteins resistant to proteolytic enzymes ensures the state of local passive immunity in neonates receiving breast milk.", "contents": "[Natural feeding and its importance in forming local immunological protection in the gastrointestinal tract during the neonatal period]. The immune proteins content in the breast milk at the 1st week of lactation as contrasted to the data obtained by investigating immunoglobulins in feces of newborns breast-fed was studied. Both in the breast milk and in feces the A-class immunoglobulins being of great importance in the immunological protection of the mucosae prevail. A high level of immune proteins resistant to proteolytic enzymes ensures the state of local passive immunity in neonates receiving breast milk."}
{"id": "PMID:462887", "title": "[Dietary treatment of children with phenylketonuria].", "content": "The sole method of treating phenylketonuria, a grave hereditary illness, is at present dietary cure. A principle for formulating therapeutic rations in this pathology is set forth, and approximate menus for children of different age groups are presented. Exclusion from the ration of an ill children of basic proteinaceous products securing a normal growth and development of the developing organism, requires a painstaking observence of the proposed rations. As the main source of protein at the time of the patient's treatment various protein hydrolysates are used. A way of correcting curative diets for children of various age is indicated. The treatment should be conducted under a strict biochemical control.", "contents": "[Dietary treatment of children with phenylketonuria]. The sole method of treating phenylketonuria, a grave hereditary illness, is at present dietary cure. A principle for formulating therapeutic rations in this pathology is set forth, and approximate menus for children of different age groups are presented. Exclusion from the ration of an ill children of basic proteinaceous products securing a normal growth and development of the developing organism, requires a painstaking observence of the proposed rations. As the main source of protein at the time of the patient's treatment various protein hydrolysates are used. A way of correcting curative diets for children of various age is indicated. The treatment should be conducted under a strict biochemical control."}
{"id": "PMID:462889", "title": "[Soluble milk protein concentrates, casecites, for infant and dietetic nutrition].", "content": "A soluble form of caseins, so-called casecites for an infant and dietetic nutrition, is obtained from separated milk by way of solving casein through using freshly precipitated lactic acid terment of 0.1 n HCl, mixed with solts-solvents. The lactoproteinic concentrates-casecites have an elevated biological value, called forth by the content of large amounts (80.0 per cent) of complete lactic protein, the presence of citrate-ions, forming as a physiologically active additive, balanced most important mineral elements. A minimal (not more than 2.0 per cent) of the lactose content in casecites enables the latter to be utilized for nutrition of persons suffering from lactose intolerance as well as to orientally regulate the carbohydrate composition of the products obtained on their basis.", "contents": "[Soluble milk protein concentrates, casecites, for infant and dietetic nutrition]. A soluble form of caseins, so-called casecites for an infant and dietetic nutrition, is obtained from separated milk by way of solving casein through using freshly precipitated lactic acid terment of 0.1 n HCl, mixed with solts-solvents. The lactoproteinic concentrates-casecites have an elevated biological value, called forth by the content of large amounts (80.0 per cent) of complete lactic protein, the presence of citrate-ions, forming as a physiologically active additive, balanced most important mineral elements. A minimal (not more than 2.0 per cent) of the lactose content in casecites enables the latter to be utilized for nutrition of persons suffering from lactose intolerance as well as to orientally regulate the carbohydrate composition of the products obtained on their basis."}
{"id": "PMID:462895", "title": "Doa (Dombrock) blood group antigen in the Japanese.", "content": "Blood samples from 100 unrelated Japanese were tested for Doa antigen. It was found that the frequency of the antigen in the Japanese (18%) is lower by far than in Caucasians, Negroes or American Indians.", "contents": "Doa (Dombrock) blood group antigen in the Japanese. Blood samples from 100 unrelated Japanese were tested for Doa antigen. It was found that the frequency of the antigen in the Japanese (18%) is lower by far than in Caucasians, Negroes or American Indians."}
{"id": "PMID:462890", "title": "[Protein metabolic indices in young healthy men with regular standardized nutrition].", "content": "Total protein and protein fractions in the blood serum, ultimate nitrogen metabolism products in urine and also the assimilation and nitrogen balance in practically healthy male-students, aged 20--25 years, were studied. Investigations proceeded against the background of rations evolved in keeping with approved physiological standard allowances covering the needs of students for nutrients and energy (1968). The registered individual values of the protein metabolism help detail the acceptance of standards for a given group of the population.", "contents": "[Protein metabolic indices in young healthy men with regular standardized nutrition]. Total protein and protein fractions in the blood serum, ultimate nitrogen metabolism products in urine and also the assimilation and nitrogen balance in practically healthy male-students, aged 20--25 years, were studied. Investigations proceeded against the background of rations evolved in keeping with approved physiological standard allowances covering the needs of students for nutrients and energy (1968). The registered individual values of the protein metabolism help detail the acceptance of standards for a given group of the population."}
{"id": "PMID:462891", "title": "[Energy demand of track-and-field weight throwers].", "content": "In sportsmen-throwers the matinal exchange of rest in conditions of sport gatherings is found to be fairly high. The energy spent in a single training comprised 723--910 kcal in women and 829--975 kcal in men. In these conditions the diurnal energy spent by throwers, was 4000--4500 kcal in women and 4500--5000 kcal in men. The energy spent for the rest exchange and physical trainings comprised 70--80 per cent of the entire energy spent by the throwers in 24 hours. The caloric value of the diurnal ration was in the range of 4000--5000 kcal.", "contents": "[Energy demand of track-and-field weight throwers]. In sportsmen-throwers the matinal exchange of rest in conditions of sport gatherings is found to be fairly high. The energy spent in a single training comprised 723--910 kcal in women and 829--975 kcal in men. In these conditions the diurnal energy spent by throwers, was 4000--4500 kcal in women and 4500--5000 kcal in men. The energy spent for the rest exchange and physical trainings comprised 70--80 per cent of the entire energy spent by the throwers in 24 hours. The caloric value of the diurnal ration was in the range of 4000--5000 kcal."}
{"id": "PMID:462896", "title": "HLA antigens in genetic neutropenia of Yemenite Jews.", "content": "The HLA antigenic system was studied in Yemenite Jews with genetic neutropenia. No deviation in antigen frequency was observed when compared with matched controls from the same ethnic group. HLA antigen frequency and common haplotypes of the Yemenite Jews were found entirely different from those of the African blacks, known to have the same anomaly.", "contents": "HLA antigens in genetic neutropenia of Yemenite Jews. The HLA antigenic system was studied in Yemenite Jews with genetic neutropenia. No deviation in antigen frequency was observed when compared with matched controls from the same ethnic group. HLA antigen frequency and common haplotypes of the Yemenite Jews were found entirely different from those of the African blacks, known to have the same anomaly."}
{"id": "PMID:462897", "title": "Anti-HBe and subtypes of HBsAg among asymptomatic HBsAg carriers.", "content": "Finnish HBsAg positive blood donors were analyzed for the HBsAg subtype distribution and the presence of e antigen (HBeAg) and antibody to e (anti-HBe). 88% (159 of 180) of the donors had subtype and ad and 12% (21 of 180) had ay. HBeAg was found in 1% (2 of 180) and anti-HBe in 74% (133 of 180) of the donors. Anti-HBe was significantly (p less than 0.0001) more common among donors with subtype ad than with ay: 80 and 29% respectively. The mean age of donors with both subtype ad and anti-HBe was significantly (p less than 0.001) lower than that of the other donor categories.", "contents": "Anti-HBe and subtypes of HBsAg among asymptomatic HBsAg carriers. Finnish HBsAg positive blood donors were analyzed for the HBsAg subtype distribution and the presence of e antigen (HBeAg) and antibody to e (anti-HBe). 88% (159 of 180) of the donors had subtype and ad and 12% (21 of 180) had ay. HBeAg was found in 1% (2 of 180) and anti-HBe in 74% (133 of 180) of the donors. Anti-HBe was significantly (p less than 0.0001) more common among donors with subtype ad than with ay: 80 and 29% respectively. The mean age of donors with both subtype ad and anti-HBe was significantly (p less than 0.001) lower than that of the other donor categories."}
{"id": "PMID:462894", "title": "[Methods of computing the content of individual fatty acids in food products].", "content": "Two ways of calculating the content of individual fatty acids in the foods based on the data of gas liquid and thin-layer chromatography (through average values calculation) or only on those of the first (through addition of standard) are advisable. Most reliable in the last case is addition of trimargarine as a standard. The evaluation of the calculation errors is given. A variation of acidic-alkaline methanolysis of fat and esterification of free fatty acids in a closed system is proposed. By using this procedure the yield of methyl esters of fatty acids forms 90 +/- 7 per cent.", "contents": "[Methods of computing the content of individual fatty acids in food products]. Two ways of calculating the content of individual fatty acids in the foods based on the data of gas liquid and thin-layer chromatography (through average values calculation) or only on those of the first (through addition of standard) are advisable. Most reliable in the last case is addition of trimargarine as a standard. The evaluation of the calculation errors is given. A variation of acidic-alkaline methanolysis of fat and esterification of free fatty acids in a closed system is proposed. By using this procedure the yield of methyl esters of fatty acids forms 90 +/- 7 per cent."}
{"id": "PMID:462892", "title": "[Lipid metabolic indices in the liver, blood and bile while using diets with a low protein content and varying carbohydrate and fat ratio].", "content": "In tests with rats observations over changes in the lipids metabolism in the liver, blood and bile with application of low-protein diets and different proportions of carbohydrates and fat were carried out. The No-1 ration containing 5 per cent of protein and an augmented to 70 per cent carbohydrates (starch) amount led to accumulation in the liver and to increased radioactivity in triglycerides and ether-bound cholesterol, to diminution of the amount and radioactivity of phospholipids in the liver and blood and to a lowered lipids complex concentration in the bile of cholic acid and proportions of glycoconjugates and tauroconjugates. The No-2 ration, where a greater carbohydrates quota was represented by a mixture of starch and saccharose, and also the No-3 ration with an elevated share of fat, produced similar, but to a higher degree, pronounced changes in the liver, blood and bile.", "contents": "[Lipid metabolic indices in the liver, blood and bile while using diets with a low protein content and varying carbohydrate and fat ratio]. In tests with rats observations over changes in the lipids metabolism in the liver, blood and bile with application of low-protein diets and different proportions of carbohydrates and fat were carried out. The No-1 ration containing 5 per cent of protein and an augmented to 70 per cent carbohydrates (starch) amount led to accumulation in the liver and to increased radioactivity in triglycerides and ether-bound cholesterol, to diminution of the amount and radioactivity of phospholipids in the liver and blood and to a lowered lipids complex concentration in the bile of cholic acid and proportions of glycoconjugates and tauroconjugates. The No-2 ration, where a greater carbohydrates quota was represented by a mixture of starch and saccharose, and also the No-3 ration with an elevated share of fat, produced similar, but to a higher degree, pronounced changes in the liver, blood and bile."}
{"id": "PMID:462899", "title": "Incidence of allo-immunization and allergic reactions to cryoprecipitate in haemophilia.", "content": "38 haemophiliacs who had received frequent cryoprecipitate infusions, were investigated for the presence of antibodies directed against red cell, HLA, Gm, Inv and platelet-specific antigens. 15 had detectable antibodies against one or more antigen systems. 11 experienced allergic reactions following infusions of cryoprecipitate. There was no correlation between allergic reactions and the presence of detectable antibodies.", "contents": "Incidence of allo-immunization and allergic reactions to cryoprecipitate in haemophilia. 38 haemophiliacs who had received frequent cryoprecipitate infusions, were investigated for the presence of antibodies directed against red cell, HLA, Gm, Inv and platelet-specific antigens. 15 had detectable antibodies against one or more antigen systems. 11 experienced allergic reactions following infusions of cryoprecipitate. There was no correlation between allergic reactions and the presence of detectable antibodies."}
{"id": "PMID:462900", "title": "Another Kell-related antibody, anti-K19.", "content": "A new high-frequency antigen related to the Kell system was defined by the serum of a patient, Mrs. Sub. The antigen was lacking from her cells and those of one of her three siblings. All random cells tested were incompatible with her serum as were bloods of other various \"null\" phenotypes, but Ko bloods were compatible suggesting that the antibody, subsequently called anti-K19, was related to the Kell blood group system.", "contents": "Another Kell-related antibody, anti-K19. A new high-frequency antigen related to the Kell system was defined by the serum of a patient, Mrs. Sub. The antigen was lacking from her cells and those of one of her three siblings. All random cells tested were incompatible with her serum as were bloods of other various \"null\" phenotypes, but Ko bloods were compatible suggesting that the antibody, subsequently called anti-K19, was related to the Kell blood group system."}
{"id": "PMID:462901", "title": "Sensitivities of radioimmunoassay and enzyme-linked immunosorbent assay for detection of hepatitis B surface antigen.", "content": "Radioimmunoassay (RIA) using Austria II (Abbott) was found to be more sensitive for the detection of HBsAg than the Hepanostika Micro-Elisa system (Organon Teknika), by a factor of 5- to 20-fold for subtype 'ad' (with one exception), and 2- to 5-fold for subtype 'ay'. The sensitivities of RIA and the enzyme-linked immunosorbent assay (Elisa) have also been evaluated in the screening of 178 selected serum samples contributed by 7 different laboratories. Of these, 149 were found to be positive for HBsAg by RIA, 142 were positive by Elisa and 36 were positive by CEP.", "contents": "Sensitivities of radioimmunoassay and enzyme-linked immunosorbent assay for detection of hepatitis B surface antigen. Radioimmunoassay (RIA) using Austria II (Abbott) was found to be more sensitive for the detection of HBsAg than the Hepanostika Micro-Elisa system (Organon Teknika), by a factor of 5- to 20-fold for subtype 'ad' (with one exception), and 2- to 5-fold for subtype 'ay'. The sensitivities of RIA and the enzyme-linked immunosorbent assay (Elisa) have also been evaluated in the screening of 178 selected serum samples contributed by 7 different laboratories. Of these, 149 were found to be positive for HBsAg by RIA, 142 were positive by Elisa and 36 were positive by CEP."}
{"id": "PMID:462902", "title": "Side products of routine plasma fractionation. I. Serum cholinesterase (EC 3.1.1.8).", "content": "Cholinesterase (EC 3.1.1.8) for intravenous use has been obtained from fraction IV, a by-product of ehtanol fractionation. A four-step procedure has been developed associating precipitation by Rivanol and PEG 4000 with chromatography on DEAE-cellulose. The yield corresponds to 30--35% of the activity found in fraction  IV. Vials containing 5000 U of CHE stabilized by 2.5% albumin have been prepared. The pyrogen-free product is well tolerated in the human.", "contents": "Side products of routine plasma fractionation. I. Serum cholinesterase (EC 3.1.1.8). Cholinesterase (EC 3.1.1.8) for intravenous use has been obtained from fraction IV, a by-product of ehtanol fractionation. A four-step procedure has been developed associating precipitation by Rivanol and PEG 4000 with chromatography on DEAE-cellulose. The yield corresponds to 30--35% of the activity found in fraction  IV. Vials containing 5000 U of CHE stabilized by 2.5% albumin have been prepared. The pyrogen-free product is well tolerated in the human."}
{"id": "PMID:462903", "title": "Distribution of H antigen in persons of blood groups A, B, and AB.", "content": "The distribution of H antigen in tissues of persons of blood groups A, B, and AB was examined. H antigen could be demonstrated in all tissues in which the isologous antigens A and B were demonstrated, although some cells demonstrated isologous but not H antigen. In most tissues, areas were seen that demonstrated H antigen but not the isologous antigen. This was especially true in Brunner's glands of the duodenum and esophageal glands, where the amount of H antigen was apparently greater than the amount of the isologous antigen. Since the results varied greatly from tissue to tissue, it was not possible to interpret the data with respect to the biochemical pathways of blood group antigen formation.", "contents": "Distribution of H antigen in persons of blood groups A, B, and AB. The distribution of H antigen in tissues of persons of blood groups A, B, and AB was examined. H antigen could be demonstrated in all tissues in which the isologous antigens A and B were demonstrated, although some cells demonstrated isologous but not H antigen. In most tissues, areas were seen that demonstrated H antigen but not the isologous antigen. This was especially true in Brunner's glands of the duodenum and esophageal glands, where the amount of H antigen was apparently greater than the amount of the isologous antigen. Since the results varied greatly from tissue to tissue, it was not possible to interpret the data with respect to the biochemical pathways of blood group antigen formation."}
{"id": "PMID:462904", "title": "Auto-immune hemolytic anemia caused by anti-K13.", "content": "A case of auto-immune hemolytic anemia caused by auto anti-K13 is described. Direct antiglobulin tests using monospecific reagents showed that IgG and the C3 component of complement were present on the patient's red cells. Eluted auto-antibody did not react with Ko or with K:--13 cells. Kell blood group antigenicity of the patient's red cells did not appear to be reduced.", "contents": "Auto-immune hemolytic anemia caused by anti-K13. A case of auto-immune hemolytic anemia caused by auto anti-K13 is described. Direct antiglobulin tests using monospecific reagents showed that IgG and the C3 component of complement were present on the patient's red cells. Eluted auto-antibody did not react with Ko or with K:--13 cells. Kell blood group antigenicity of the patient's red cells did not appear to be reduced."}
{"id": "PMID:462906", "title": "Oka: an erythrocytic antigen of high frequency.", "content": "Clinically significant antibodies to a 'new' high-frequency antigen on red blood cells have been detected in the serum of a Japanese woman. The notation Oka has been given to this antibody and its corresponding antigen. Two of the patient's siblings are Ok (a--) but a large number of red cell samples lacking common antigens were tested and found to be Ok (a+). The antibody reacted by the antiglobulin test with blood samples from random blood donors and with more than 4,000 red cell samples from Oriental blood donors and patients.", "contents": "Oka: an erythrocytic antigen of high frequency. Clinically significant antibodies to a 'new' high-frequency antigen on red blood cells have been detected in the serum of a Japanese woman. The notation Oka has been given to this antibody and its corresponding antigen. Two of the patient's siblings are Ok (a--) but a large number of red cell samples lacking common antigens were tested and found to be Ok (a+). The antibody reacted by the antiglobulin test with blood samples from random blood donors and with more than 4,000 red cell samples from Oriental blood donors and patients."}
{"id": "PMID:462907", "title": "The influence of ionic strength, albumin and incubation time on the sensitivity of the indirect Coombs' test.", "content": "The sensitivity of the indirect Coombs' test was investigated using saline, albumin, or low ionic strength (LIS) in the incubation phase and an incubation time varying from 5 to 120 min. Titration of 41 antibodies showed that LIS always resulted in a higher or almost the same mean scores as the other solutions after the same incubation time. The maximum mean score was always higher with LIS than with saline and was reached after a shorter period of incubation, only 20--40 min even with the weakest antibodies. With LIS, a longer incubation time than 40 min resulted in a sudden decrease in mean score. The maximum mean score using saline or albumin was reached after 40--60 min incubation. The same mean score was obtained after only 15--20 min, when LIS was used. Albumin gave only slightly higher mean scores than saline.", "contents": "The influence of ionic strength, albumin and incubation time on the sensitivity of the indirect Coombs' test. The sensitivity of the indirect Coombs' test was investigated using saline, albumin, or low ionic strength (LIS) in the incubation phase and an incubation time varying from 5 to 120 min. Titration of 41 antibodies showed that LIS always resulted in a higher or almost the same mean scores as the other solutions after the same incubation time. The maximum mean score was always higher with LIS than with saline and was reached after a shorter period of incubation, only 20--40 min even with the weakest antibodies. With LIS, a longer incubation time than 40 min resulted in a sudden decrease in mean score. The maximum mean score using saline or albumin was reached after 40--60 min incubation. The same mean score was obtained after only 15--20 min, when LIS was used. Albumin gave only slightly higher mean scores than saline."}
{"id": "PMID:462908", "title": "Distribution of subtypes of hepatitis B surface antigen in Western India.", "content": "HBsAg subtype distribution of 105 positive samples showed 91.4% as ay type. The ay was predominant in different disease conditions, healthy carriers and posttransfusion hepatitis. The prevalence of ay in the Bombay sample is in contrast to the predominance of ad reported from North India. These findings may suggest multifocal infection in different regions of India.", "contents": "Distribution of subtypes of hepatitis B surface antigen in Western India. HBsAg subtype distribution of 105 positive samples showed 91.4% as ay type. The ay was predominant in different disease conditions, healthy carriers and posttransfusion hepatitis. The prevalence of ay in the Bombay sample is in contrast to the predominance of ad reported from North India. These findings may suggest multifocal infection in different regions of India."}
{"id": "PMID:462909", "title": "Mechanisms of vasomotor reactions in the use of SPPS.", "content": "Vasomotor reactions have been reported during infusions of stable plasma protein solutions (SPPS). Possible mechanisms of this phenomenon have been studied using rabbit ear vein preparations. Most samples of SPPS were found inactive but could be activated by proteolytic enzymes. Two factors appear to be responsible: one has kinin-like properties and the other behaves as kininogen. The latter could be activated by proteases released from both granulocytes and platelets and it is suggested that such activation may occur in clinical use. The bioassay employed in this study provides a basis for modifications in production of SPPS to eliminate these factors.", "contents": "Mechanisms of vasomotor reactions in the use of SPPS. Vasomotor reactions have been reported during infusions of stable plasma protein solutions (SPPS). Possible mechanisms of this phenomenon have been studied using rabbit ear vein preparations. Most samples of SPPS were found inactive but could be activated by proteolytic enzymes. Two factors appear to be responsible: one has kinin-like properties and the other behaves as kininogen. The latter could be activated by proteases released from both granulocytes and platelets and it is suggested that such activation may occur in clinical use. The bioassay employed in this study provides a basis for modifications in production of SPPS to eliminate these factors."}
{"id": "PMID:462910", "title": "Acquired B antigen, Tk activation and A1 destroying enzyme activity in a patient with septicaemia.", "content": "During the course of septicaemia, a group A1 patient developed several RBC antigenic modifications. Acquired B antigen, Tk transformation and a partial disappearance of A1 receptors associated with an increase of H reactivity were observed.", "contents": "Acquired B antigen, Tk activation and A1 destroying enzyme activity in a patient with septicaemia. During the course of septicaemia, a group A1 patient developed several RBC antigenic modifications. Acquired B antigen, Tk transformation and a partial disappearance of A1 receptors associated with an increase of H reactivity were observed."}
{"id": "PMID:462911", "title": "Studies on the preservation of red blood cells. Attachment of the third component of human complement to erythrocytes during storage at 4 degrees C.", "content": "Utilizing an automated antiglobulin test, we have investigated the presence of the third and fourth components of human complement on normal red blood cells (RBCs). Only negligible amounts of the fourth component, C4, could be detected on either freshly collected or stored RBCs. The fragment C3d of the third component, C3, was detectable on both freshly collected and stored normal RBCs. A product derived from C3 and reacting with anti-C3c antibody was only barely detectable on freshly collected normal RBCs. During storage of blood at 4 degrees C, increasing quantities of this material were detected on the RBC membrane. Bromelin treatment rendered stored RBCs completely nonreactive with anit-C3c antibody, whereas only partial loss of reactivity was observed following incubation with heated plasma. In contrast, incubation of EC43 with heated plasma completely abolished their ability to react with anti-C3c antibody. We suggest that the presence of this C3 fragment on stored RBCs may contribute to the development of \"preservation injury\".", "contents": "Studies on the preservation of red blood cells. Attachment of the third component of human complement to erythrocytes during storage at 4 degrees C. Utilizing an automated antiglobulin test, we have investigated the presence of the third and fourth components of human complement on normal red blood cells (RBCs). Only negligible amounts of the fourth component, C4, could be detected on either freshly collected or stored RBCs. The fragment C3d of the third component, C3, was detectable on both freshly collected and stored normal RBCs. A product derived from C3 and reacting with anti-C3c antibody was only barely detectable on freshly collected normal RBCs. During storage of blood at 4 degrees C, increasing quantities of this material were detected on the RBC membrane. Bromelin treatment rendered stored RBCs completely nonreactive with anit-C3c antibody, whereas only partial loss of reactivity was observed following incubation with heated plasma. In contrast, incubation of EC43 with heated plasma completely abolished their ability to react with anti-C3c antibody. We suggest that the presence of this C3 fragment on stored RBCs may contribute to the development of \"preservation injury\"."}
{"id": "PMID:462912", "title": "Lognormal distribution of HBsAg strengths in an English population.", "content": "The frequency distribution of reverse passive haemagglutination titres for HBsAg in the sera of 78 positive blood donors found furing 2.5-year period closely follows a lognormal distribution curve. This lognormal distribution suggests that the total population of HBsAg-positive donors during this period was approximately 84. The modified Hepatest now in use will be expected to detect 82 of these, and to miss only 1 HBsAg-positive donor in 50,000 screening tests of Manchester blood donors.", "contents": "Lognormal distribution of HBsAg strengths in an English population. The frequency distribution of reverse passive haemagglutination titres for HBsAg in the sera of 78 positive blood donors found furing 2.5-year period closely follows a lognormal distribution curve. This lognormal distribution suggests that the total population of HBsAg-positive donors during this period was approximately 84. The modified Hepatest now in use will be expected to detect 82 of these, and to miss only 1 HBsAg-positive donor in 50,000 screening tests of Manchester blood donors."}
{"id": "PMID:462913", "title": "Detection of hepatitis B surface antigen with the miniature centrifugal fast analyzer. A modified reversed passive hemagglutination procedure.", "content": "A modified reversed passive hemagglutination test for the detection of hepatitis B surface antigen HBsAg is described. Sera and reagent cells coated with antibody to HBsAg (anti-HBs) are loaded separately into the rotor of a miniature centrifugal fast analyzer. The rotor is centrifuged briefly to transfer the components into its cuvettes. After mixing, the suspensions are allowed to stand at room temperature for 30 min, following which the rotor is again centrifuged and the absorbance of each cuvette is monitored. Cells suspended in serum containing HBsAg leave the light path more rapidly than cells suspended in sera free of antigen. The magnitude of change in absorbance varies directly with the concentration of the antigen. In 45 sera tested by the conventional V-plate technique, findings were as follows: 21 positive, 19 false positive and 5 negative. The automated procedure unequivocally differentiated the 21 positives; results for the false positive and negative specimens were identical and clearly distinguishable from the positive results. The automated procedure enhances specificity, offers equivalent sensitivity, and results that are quantitative and objective.", "contents": "Detection of hepatitis B surface antigen with the miniature centrifugal fast analyzer. A modified reversed passive hemagglutination procedure. A modified reversed passive hemagglutination test for the detection of hepatitis B surface antigen HBsAg is described. Sera and reagent cells coated with antibody to HBsAg (anti-HBs) are loaded separately into the rotor of a miniature centrifugal fast analyzer. The rotor is centrifuged briefly to transfer the components into its cuvettes. After mixing, the suspensions are allowed to stand at room temperature for 30 min, following which the rotor is again centrifuged and the absorbance of each cuvette is monitored. Cells suspended in serum containing HBsAg leave the light path more rapidly than cells suspended in sera free of antigen. The magnitude of change in absorbance varies directly with the concentration of the antigen. In 45 sera tested by the conventional V-plate technique, findings were as follows: 21 positive, 19 false positive and 5 negative. The automated procedure unequivocally differentiated the 21 positives; results for the false positive and negative specimens were identical and clearly distinguishable from the positive results. The automated procedure enhances specificity, offers equivalent sensitivity, and results that are quantitative and objective."}
{"id": "PMID:462914", "title": "Influence of glygerol on ATP and 2,3-DPG levels of human erythrocytes.", "content": "Glycerolization of freshly collected human erythrocytes leads to a reduction in ATP levels which return to their original values following deglycerolization. The reduction in ATP levels is largely prevented by pyruvate but not by inosine and glucose, singly or in combination. This implies that the ATP lesion associated with glycerolization reflects mainly a decrease in the NAD/NADH ratio and accordingly a reduced glyceraldehyde-3-phosphate dehydrogenase activity. 2,3-DPG levels were not influenced by glycerolization and deglycerolization. Erythrocytes depleted of ATP in the presence of glycerol can have their ATP levels repleted by either pyruvate or inosine, but require the presence of both compounds for maintenance during a post-rejuvenation incubation period. This indicates that the prolonged presence of glycerol influences both the NAD/NADH ratio and the carbon-containing intermediates of glycolysis. Glycerol did not influence ATP repletion of erythrocytes stored at 4 degrees C for 20 days by inosine in combination with pyruvate, but substantially decreased the repletion of their 2,3-DPG levels.", "contents": "Influence of glygerol on ATP and 2,3-DPG levels of human erythrocytes. Glycerolization of freshly collected human erythrocytes leads to a reduction in ATP levels which return to their original values following deglycerolization. The reduction in ATP levels is largely prevented by pyruvate but not by inosine and glucose, singly or in combination. This implies that the ATP lesion associated with glycerolization reflects mainly a decrease in the NAD/NADH ratio and accordingly a reduced glyceraldehyde-3-phosphate dehydrogenase activity. 2,3-DPG levels were not influenced by glycerolization and deglycerolization. Erythrocytes depleted of ATP in the presence of glycerol can have their ATP levels repleted by either pyruvate or inosine, but require the presence of both compounds for maintenance during a post-rejuvenation incubation period. This indicates that the prolonged presence of glycerol influences both the NAD/NADH ratio and the carbon-containing intermediates of glycolysis. Glycerol did not influence ATP repletion of erythrocytes stored at 4 degrees C for 20 days by inosine in combination with pyruvate, but substantially decreased the repletion of their 2,3-DPG levels."}
{"id": "PMID:462915", "title": "A new low incidence red blood cell antigen, Pe.", "content": "A new low incidence red cell antigen (Pe), and its identifying IgG antibody are described. The antigen is destroyed by enzymes and is absent from serum, urine, saliva, and platelets. The Pe gene frequency is estimated to be less than 0.0003, and the Pe locus does not appear to be linked to Rh or Fy, nor carried by the X or Y chromosomes. The clinical significance of this antibody remains to be determined.", "contents": "A new low incidence red blood cell antigen, Pe. A new low incidence red cell antigen (Pe), and its identifying IgG antibody are described. The antigen is destroyed by enzymes and is absent from serum, urine, saliva, and platelets. The Pe gene frequency is estimated to be less than 0.0003, and the Pe locus does not appear to be linked to Rh or Fy, nor carried by the X or Y chromosomes. The clinical significance of this antibody remains to be determined."}
{"id": "PMID:462916", "title": "Antibody-mediated immunosuppression. Comparison of syngeneic and allogeneic antibodies.", "content": "Two major histocompatible strains of rats differing at 'minor' antigens including immunoglobulin allotype disparity were used in experiments to study antibody-mediated suppression of a primary antibody response against third party allogeneic red cells. Antibodies against red cells were raised by repeated injections in the two strains of rats, and the effects of the two pools of serum to suppress antibody response upon intravenous injection of a small test dose of blood were assessed and compared in in vitro titrations. It was concluded that allotype differences, as suggested by others, were not necessary and they were not amplifying the suppression. The observations are discussed in relation to the Rh immunoprophylaxis in man.", "contents": "Antibody-mediated immunosuppression. Comparison of syngeneic and allogeneic antibodies. Two major histocompatible strains of rats differing at 'minor' antigens including immunoglobulin allotype disparity were used in experiments to study antibody-mediated suppression of a primary antibody response against third party allogeneic red cells. Antibodies against red cells were raised by repeated injections in the two strains of rats, and the effects of the two pools of serum to suppress antibody response upon intravenous injection of a small test dose of blood were assessed and compared in in vitro titrations. It was concluded that allotype differences, as suggested by others, were not necessary and they were not amplifying the suppression. The observations are discussed in relation to the Rh immunoprophylaxis in man."}
{"id": "PMID:462917", "title": "Development of large scale fractionation methods. VII. Preparation of antithrombin III concentrate.", "content": "A large scale method for preparation of antithrombin III (AT III) concentrate from plasma or from Cohn fraction IV-1 (Fr. IV-1) has been described. It consists of the following steps: (a) partial purification by precipitation of impurities with 20% polyethylene glycol (PEG) 4000; (b) isolation of AT III from the PEG supernatant by batch adsorption and elution on heparin-Sepharose at a ratio corresponding to 45 vol of plasma or 80 vol of 10% Fr. IV-1 solution to 1 vol of gel; (c) concentration and desalting of the eluted AT III on a Pellicon ultrafiltration system; (d) pasteurization of AT III concentrate by heating for 10 h at 60 degrees C in the presence of 0.5 M sodium citrate at pH 7.5; (e) removal of excess citrate by gel filtration on Sephadex G-50; and (f) sterile filtration, filling and lyophilization. The recovery by activity was 32% from a 113-liter plasma batch and 16% from a 42-kg Fr. IV-1 batch. Both AT III concentrates, derived either from plasma or from Fr. IV-1, had similar specific activity and electrophoretic purity, were nonpyrogenic and met all other FDA requirements for biologic products. Pasteurization induced changes in disc gel and isotachophoretic patterns of AT III preparations.", "contents": "Development of large scale fractionation methods. VII. Preparation of antithrombin III concentrate. A large scale method for preparation of antithrombin III (AT III) concentrate from plasma or from Cohn fraction IV-1 (Fr. IV-1) has been described. It consists of the following steps: (a) partial purification by precipitation of impurities with 20% polyethylene glycol (PEG) 4000; (b) isolation of AT III from the PEG supernatant by batch adsorption and elution on heparin-Sepharose at a ratio corresponding to 45 vol of plasma or 80 vol of 10% Fr. IV-1 solution to 1 vol of gel; (c) concentration and desalting of the eluted AT III on a Pellicon ultrafiltration system; (d) pasteurization of AT III concentrate by heating for 10 h at 60 degrees C in the presence of 0.5 M sodium citrate at pH 7.5; (e) removal of excess citrate by gel filtration on Sephadex G-50; and (f) sterile filtration, filling and lyophilization. The recovery by activity was 32% from a 113-liter plasma batch and 16% from a 42-kg Fr. IV-1 batch. Both AT III concentrates, derived either from plasma or from Fr. IV-1, had similar specific activity and electrophoretic purity, were nonpyrogenic and met all other FDA requirements for biologic products. Pasteurization induced changes in disc gel and isotachophoretic patterns of AT III preparations."}
{"id": "PMID:462918", "title": "Improved yields of factor VIII from heparinized plasma.", "content": "The Factor VIII procoagulant activity in plasma, cryoprecipitate, and their polyethylene glycol (PEG) precipitates is markedly increased if blood is collected into heparin rather than into citrate phosphate dextrose (CPD). There is a 34% increase in the initial level of the Factor VIII activity in the heparinized plasma with 78% of this initial activity (184 U) recovered in the cryoprecipitated. As well, the stability of the Factor VIII activity is improved: after 24 h of incubation at 22 degrees C, 99% of the initial activity is retained in heparinized plasma whereas only 64% remains in CPD plasma. The cryoprecipitate prepared from heparinized plasma is equally stable after 24 h. The PEG concentrate prepared from the cryoprecipitate of heparinized plasma is increased to 128 U compared to only 54 U from CPD plasma. Relative recoveries were 531 U/l for heparinized plasma versus 215 U/l for CPD plasma. This represents a 147% increase in yield.", "contents": "Improved yields of factor VIII from heparinized plasma. The Factor VIII procoagulant activity in plasma, cryoprecipitate, and their polyethylene glycol (PEG) precipitates is markedly increased if blood is collected into heparin rather than into citrate phosphate dextrose (CPD). There is a 34% increase in the initial level of the Factor VIII activity in the heparinized plasma with 78% of this initial activity (184 U) recovered in the cryoprecipitated. As well, the stability of the Factor VIII activity is improved: after 24 h of incubation at 22 degrees C, 99% of the initial activity is retained in heparinized plasma whereas only 64% remains in CPD plasma. The cryoprecipitate prepared from heparinized plasma is equally stable after 24 h. The PEG concentrate prepared from the cryoprecipitate of heparinized plasma is increased to 128 U compared to only 54 U from CPD plasma. Relative recoveries were 531 U/l for heparinized plasma versus 215 U/l for CPD plasma. This represents a 147% increase in yield."}
{"id": "PMID:462920", "title": "A simple method for the preparation of human serum albumin.", "content": "A simple method, based on ethanol fractionation, for the preparation of highly purified human serum albumin with a higher yield than that of the conventional ethanol procedures is described. It consists of two purification steps, namely, precipitation of most of the other plasma proteins from a 3-fold diluted plasma with ethanol at 42% concentration, pH 5.75 and -5 degrees C, leaving over 96% pure albumin in the supernatant, followed by isoelectric precipitation of albumin from the supernatant at pH 4.8 and -5 degrees C. The paste thus obtained was processed to the final albumin solution according to the conventional methods. The yield of the final albumin with a purity of over 99% was equivalent to 29.5 g/l of plasma representing a recovery of over 93%. The possibility of recovering other plasma proteins and the suitability for large scale preparation are also discussed.", "contents": "A simple method for the preparation of human serum albumin. A simple method, based on ethanol fractionation, for the preparation of highly purified human serum albumin with a higher yield than that of the conventional ethanol procedures is described. It consists of two purification steps, namely, precipitation of most of the other plasma proteins from a 3-fold diluted plasma with ethanol at 42% concentration, pH 5.75 and -5 degrees C, leaving over 96% pure albumin in the supernatant, followed by isoelectric precipitation of albumin from the supernatant at pH 4.8 and -5 degrees C. The paste thus obtained was processed to the final albumin solution according to the conventional methods. The yield of the final albumin with a purity of over 99% was equivalent to 29.5 g/l of plasma representing a recovery of over 93%. The possibility of recovering other plasma proteins and the suitability for large scale preparation are also discussed."}
{"id": "PMID:462921", "title": "[Analysis of the RNA in different strains of the influenza A virus].", "content": "The degree of affinity of influenza A/WSN/33 and A/Singapore/57 virus genomes was studied by the method of molecular hybridization which showed the presence in their genomes of 54--57% of analogous sequences. The electrophoretic analysis of RNA--RNA hybrids formed in hybridization of both homologous and heterologous virion and virus-induced RNAs of influenza viruses revealed significant differences in base sequences of the IV, V, and VIII fragments of influenza A/WSN/33 virus RNA and the corresponding fragments of influenza A/Singapore/57 virus RNA; the III and VII fragments of these strains have sites also differing in base sequences at the ends of molecules.", "contents": "[Analysis of the RNA in different strains of the influenza A virus]. The degree of affinity of influenza A/WSN/33 and A/Singapore/57 virus genomes was studied by the method of molecular hybridization which showed the presence in their genomes of 54--57% of analogous sequences. The electrophoretic analysis of RNA--RNA hybrids formed in hybridization of both homologous and heterologous virion and virus-induced RNAs of influenza viruses revealed significant differences in base sequences of the IV, V, and VIII fragments of influenza A/WSN/33 virus RNA and the corresponding fragments of influenza A/Singapore/57 virus RNA; the III and VII fragments of these strains have sites also differing in base sequences at the ends of molecules."}
{"id": "PMID:462922", "title": "[Structure of the genomes of recombinant strains of influenza A viruses with various antigenic and biological properties].", "content": "Genomes of influenza A/WSN/33, A/Singapore/57 and recombinant X-7 and X-9 viruses were studied by the method of molecular hybridization followed by electrophoretic separation of RNA-duplexes in polyacrylamide gel. The genome of the recombinant X-7 influenza virus strain was found to contain fragments V and VII of RNA corresponding to fragments of A/Singapore/57 RNA, whereas fragments III, IV, and VIII were derived from influenza A/WSN/33 virus. Recombinant X-9 had fragments III, IV, and VII of RNA similar to those of A/Singapore/57, and fragments V,VIII of RNA derived from influenza A/WSN/33 virus. Resistance to rimantadine in influenza viruses correlated in X-7 and X-9 recombinants to the strain appurtenance of fragment VIII.", "contents": "[Structure of the genomes of recombinant strains of influenza A viruses with various antigenic and biological properties]. Genomes of influenza A/WSN/33, A/Singapore/57 and recombinant X-7 and X-9 viruses were studied by the method of molecular hybridization followed by electrophoretic separation of RNA-duplexes in polyacrylamide gel. The genome of the recombinant X-7 influenza virus strain was found to contain fragments V and VII of RNA corresponding to fragments of A/Singapore/57 RNA, whereas fragments III, IV, and VIII were derived from influenza A/WSN/33 virus. Recombinant X-9 had fragments III, IV, and VII of RNA similar to those of A/Singapore/57, and fragments V,VIII of RNA derived from influenza A/WSN/33 virus. Resistance to rimantadine in influenza viruses correlated in X-7 and X-9 recombinants to the strain appurtenance of fragment VIII."}
{"id": "PMID:462923", "title": "[Ribonucleic acids and proteins of influenza A/USSR/90/77 viruses].", "content": "The \"return\" of influenza A (HINI) virus after 20 years of absence raised the question of the sources and mechanisms of emergence of epidemic influenza viruses and, particularly, of a new HINI virus (A/USSR/90/77). Two alternative hypotheses answer this question differently: the new HINI virus is the progeny of old HINI viruses retained in the human population or is a newly arising recombinant between numerous human and animal influenza viruses circulation in the biosphere. For the acceptance of one or the other hypothesis further accumulation of facts is required and, first of all, comparative investigations of RNAs and proteins of various influenza viruses. This paper presents the results of comparative studies of RNAs and proteins of old and new influenza A (HINI) viruses.", "contents": "[Ribonucleic acids and proteins of influenza A/USSR/90/77 viruses]. The \"return\" of influenza A (HINI) virus after 20 years of absence raised the question of the sources and mechanisms of emergence of epidemic influenza viruses and, particularly, of a new HINI virus (A/USSR/90/77). Two alternative hypotheses answer this question differently: the new HINI virus is the progeny of old HINI viruses retained in the human population or is a newly arising recombinant between numerous human and animal influenza viruses circulation in the biosphere. For the acceptance of one or the other hypothesis further accumulation of facts is required and, first of all, comparative investigations of RNAs and proteins of various influenza viruses. This paper presents the results of comparative studies of RNAs and proteins of old and new influenza A (HINI) viruses."}
{"id": "PMID:462924", "title": "[Analysis of the nature of virus-associated DNA in the influenza virus].", "content": "DNA sequences comprising about 5% of virus RNA were found to be present in nucleoids of influenza virus grown in chick embryos. DNA was found in two influenza virus strains under study belonging to different antigenic groups: HONI and H3N2. No DNA was demonstrated in nucleoids of a paramyxovirus, Newcastle disease virus, grown under analogous conditions. The virus-associated DNA belongs to unique sequences of cell DNA.", "contents": "[Analysis of the nature of virus-associated DNA in the influenza virus]. DNA sequences comprising about 5% of virus RNA were found to be present in nucleoids of influenza virus grown in chick embryos. DNA was found in two influenza virus strains under study belonging to different antigenic groups: HONI and H3N2. No DNA was demonstrated in nucleoids of a paramyxovirus, Newcastle disease virus, grown under analogous conditions. The virus-associated DNA belongs to unique sequences of cell DNA."}
{"id": "PMID:462925", "title": "[Antigenic and biological characteristics of influenza A1 viruses isolated in the 1977 pandemic].", "content": "Investigations of the antigenic and biological properties of the causative agents of the 1977 influenza epidemic revealed their appurtenance to the old subtype A1 of influenza virus which had disappeared from circulation 20 years ago. A comparative study of A1 viruses isolated in 1947--1956 and A1/77 established closer relationship of the latter to the causative agents of 1949 and 1950 epidemics. The \"old\" and newly isolated viruses 4ere also closely related biologically. The similarity of influenza A1/77 viruses with the agents of the pandemic cycle of A1 in 1947--1956 is a convincing confirmation of the hypothesis of Francis-Davenport and Smorodintsev-Luzyanina.", "contents": "[Antigenic and biological characteristics of influenza A1 viruses isolated in the 1977 pandemic]. Investigations of the antigenic and biological properties of the causative agents of the 1977 influenza epidemic revealed their appurtenance to the old subtype A1 of influenza virus which had disappeared from circulation 20 years ago. A comparative study of A1 viruses isolated in 1947--1956 and A1/77 established closer relationship of the latter to the causative agents of 1949 and 1950 epidemics. The \"old\" and newly isolated viruses 4ere also closely related biologically. The similarity of influenza A1/77 viruses with the agents of the pandemic cycle of A1 in 1947--1956 is a convincing confirmation of the hypothesis of Francis-Davenport and Smorodintsev-Luzyanina."}
{"id": "PMID:462931", "title": "[Express method for determining blood coagulation time].", "content": "The author established that coagulation time of capillary blood is the shorter, the longer time has elapsed from the time of pricking till the moment of blood withdrawal. That shortening of time is considerably less if the small wound made it deeper and wider. The first escaping drop coagulates most slowly. The author established that the investigation with it gives identical data with the venous blood investigation not only in healthy but also in patients with hemophilia and those treated with heparin or indirect anticoagulants. Based on those data, the author elaborated and recommended a method with capillary blood, providing identical data as those with the venous blood investigations.", "contents": "[Express method for determining blood coagulation time]. The author established that coagulation time of capillary blood is the shorter, the longer time has elapsed from the time of pricking till the moment of blood withdrawal. That shortening of time is considerably less if the small wound made it deeper and wider. The first escaping drop coagulates most slowly. The author established that the investigation with it gives identical data with the venous blood investigation not only in healthy but also in patients with hemophilia and those treated with heparin or indirect anticoagulants. Based on those data, the author elaborated and recommended a method with capillary blood, providing identical data as those with the venous blood investigations."}
{"id": "PMID:462926", "title": "[Interaction of Venezuelan equine encephalitis virus ts-mutants with cells].", "content": "An acute infection of HeLa cell cultures with a ts mutant of Venezuelan equine encephalomyelitis (VEE) virus may have different outcomes ranging from formation of a chronic virus-carrier state to complete elimination of virus from the culture. One of the possibilities is also a short-time carrier state (up to 10 passages) of the virus genome apparently existing in the form of DNA-provirus. The carrier state of virus genetic material in this case is indicated by the infectivity of cellular DNA and the presence in it of sequences homologous to those of virion RNA. The genetic material of VEE virus in HeLa cell cultures with signs of infection of the integrative type is also demonstrable by its complementation with exogenous ts-mutants.", "contents": "[Interaction of Venezuelan equine encephalitis virus ts-mutants with cells]. An acute infection of HeLa cell cultures with a ts mutant of Venezuelan equine encephalomyelitis (VEE) virus may have different outcomes ranging from formation of a chronic virus-carrier state to complete elimination of virus from the culture. One of the possibilities is also a short-time carrier state (up to 10 passages) of the virus genome apparently existing in the form of DNA-provirus. The carrier state of virus genetic material in this case is indicated by the infectivity of cellular DNA and the presence in it of sequences homologous to those of virion RNA. The genetic material of VEE virus in HeLa cell cultures with signs of infection of the integrative type is also demonstrable by its complementation with exogenous ts-mutants."}
{"id": "PMID:462932", "title": "[Argyria in the prolonged use of adsorgan].", "content": "One case of agryria is reported that developed after a long-term administration of adsorgan in a patient with dyspeptic manifestations after cholecystectomy. The developed skin syndrome -- ashen-grey discolorization of the skin of the face and bare parts of the hands and the neurological symptoms -- right monoparesis and vestibular-disturbances with clinical manifestations of chronic cholangiohepatitis, pancreatitis and gastroduodentitis -- provided considerable diagnostic difficulties. The problem of argyria is discussed as a state of intoxication in chronic use of silver-containing medicaments. The necessity of differentiating argyria by the internists is stressed upon in order to avoid it as a complication in the administration of drugs-containing silver, adsorgan resp., being applied in the physician's practice.", "contents": "[Argyria in the prolonged use of adsorgan]. One case of agryria is reported that developed after a long-term administration of adsorgan in a patient with dyspeptic manifestations after cholecystectomy. The developed skin syndrome -- ashen-grey discolorization of the skin of the face and bare parts of the hands and the neurological symptoms -- right monoparesis and vestibular-disturbances with clinical manifestations of chronic cholangiohepatitis, pancreatitis and gastroduodentitis -- provided considerable diagnostic difficulties. The problem of argyria is discussed as a state of intoxication in chronic use of silver-containing medicaments. The necessity of differentiating argyria by the internists is stressed upon in order to avoid it as a complication in the administration of drugs-containing silver, adsorgan resp., being applied in the physician's practice."}
{"id": "PMID:462927", "title": "[Modelling of viral-chemical carcinogenesis in chronic infection in mice caused by the herpes simplex type 2 virus].", "content": "The influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (HSV-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-MCA), was studied. Solid tumors developed in 20-MCA-treated mice on the side of the carcinogen administration within 81--89 days. The simultaneous administration of 70 intracerebral LD50 of HSV-2 subcutaneously into a hind leg pad and 0.1 mg 20-MCA subcutaneously into the inguinal area was accompanied by a synergistic action of the two factors. The number of tumors in the animals treated with HSV-2 in combination with 20-MCA at all intervals of observation was 1.5--2.5 times greater than in mice treated with 20-MCA alone. Mice weighing 10--12 g were more sensitive to the synergistic effect of HSV-2 and 20-MCA, whereas animals weighing 25--30 g were more sensitive to the carcinogenic effect of 20-MCA alone. No tumors developed in mice infected with HSV-2 alone by 167 days (the observation period). Mice weighing 10--12 g were found to be more sensitive to the fatal effect of HSV-2 and HSV-2 plus 20-MCA, particularly in the first 2--3 weeks after inoculation. Six months after inoculation with HSV-2 the virus was isolated from posterior root ganglia of the sacral and lumbar spinal cord by cocultivation of the ganglia with human embryo skin-muscle tissue cells.", "contents": "[Modelling of viral-chemical carcinogenesis in chronic infection in mice caused by the herpes simplex type 2 virus]. The influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (HSV-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-MCA), was studied. Solid tumors developed in 20-MCA-treated mice on the side of the carcinogen administration within 81--89 days. The simultaneous administration of 70 intracerebral LD50 of HSV-2 subcutaneously into a hind leg pad and 0.1 mg 20-MCA subcutaneously into the inguinal area was accompanied by a synergistic action of the two factors. The number of tumors in the animals treated with HSV-2 in combination with 20-MCA at all intervals of observation was 1.5--2.5 times greater than in mice treated with 20-MCA alone. Mice weighing 10--12 g were more sensitive to the synergistic effect of HSV-2 and 20-MCA, whereas animals weighing 25--30 g were more sensitive to the carcinogenic effect of 20-MCA alone. No tumors developed in mice infected with HSV-2 alone by 167 days (the observation period). Mice weighing 10--12 g were found to be more sensitive to the fatal effect of HSV-2 and HSV-2 plus 20-MCA, particularly in the first 2--3 weeks after inoculation. Six months after inoculation with HSV-2 the virus was isolated from posterior root ganglia of the sacral and lumbar spinal cord by cocultivation of the ganglia with human embryo skin-muscle tissue cells."}
{"id": "PMID:462933", "title": "[Comparative functional, scintigraphic and angiographic studies in pancreatic diseases].", "content": "Pancreas scintigraphy with 75selenomethionine, pancreocimine-secretin test and selective abdominal angiography was carried out in patients with chronic pancreatitis, pancreas carcinoma and subjects without any pancreas diseases. Scintigraphic changes in pancreas were found in 95.6 per cent of the patients with chronic pancreatitis (136 patients) in 92 per cent of them with pancreas carcinoma (25 patients) and in 53.4 per cent from the subjects without pancreas diseases (30 examined). Pathological changes in pancreatic secretion was found in 93.4 per cent of the patients with chronic pancreatitis (105 patients) in 93.8 per cent of the subjects with pancreas carcinoma (32 patients) and only in 3.3 per cent from the examined without pancreatic diseases. The angiographic examination is informative mainly in case of tumours and cysts of the pancreas. The diagnostic potentialities of the separate methods for pancreas examination were critically assessed. The basic diagnostic problems, in pancreas diseases are solved to a great extent with the combined examination with scintigraphy, pancreocimine-secretin test and angiography (76 patients).", "contents": "[Comparative functional, scintigraphic and angiographic studies in pancreatic diseases]. Pancreas scintigraphy with 75selenomethionine, pancreocimine-secretin test and selective abdominal angiography was carried out in patients with chronic pancreatitis, pancreas carcinoma and subjects without any pancreas diseases. Scintigraphic changes in pancreas were found in 95.6 per cent of the patients with chronic pancreatitis (136 patients) in 92 per cent of them with pancreas carcinoma (25 patients) and in 53.4 per cent from the subjects without pancreas diseases (30 examined). Pathological changes in pancreatic secretion was found in 93.4 per cent of the patients with chronic pancreatitis (105 patients) in 93.8 per cent of the subjects with pancreas carcinoma (32 patients) and only in 3.3 per cent from the examined without pancreatic diseases. The angiographic examination is informative mainly in case of tumours and cysts of the pancreas. The diagnostic potentialities of the separate methods for pancreas examination were critically assessed. The basic diagnostic problems, in pancreas diseases are solved to a great extent with the combined examination with scintigraphy, pancreocimine-secretin test and angiography (76 patients)."}
{"id": "PMID:462935", "title": "[Age-related aspects of gastric secretion in duodenal ulcer during the exacerbation phase (a pentagastrin study)].", "content": "The age characteristics of gastric secretion were studied in 145 patients with duodenal ulcer and 32 healthy subjects via pentagastrin. With age advancing of the patients, a gradual reduction of gastric secretion and acidity are found, being statistically significant after the age of 49. Apart of that, the average values of the indices for each age group remain considerably higher than the normal. The age, at which the disease originated, has also a certain effect on gastric secretion. Significant reduction is observed after the age of 49, concerning only secretion volume and acidity elimination at basic conditions. The changes found in the gastric secretion and acidity are admitted to possibly play a definite role for the rather unusual course and relatively rare duodenal ulcer origination at the age of 50.", "contents": "[Age-related aspects of gastric secretion in duodenal ulcer during the exacerbation phase (a pentagastrin study)]. The age characteristics of gastric secretion were studied in 145 patients with duodenal ulcer and 32 healthy subjects via pentagastrin. With age advancing of the patients, a gradual reduction of gastric secretion and acidity are found, being statistically significant after the age of 49. Apart of that, the average values of the indices for each age group remain considerably higher than the normal. The age, at which the disease originated, has also a certain effect on gastric secretion. Significant reduction is observed after the age of 49, concerning only secretion volume and acidity elimination at basic conditions. The changes found in the gastric secretion and acidity are admitted to possibly play a definite role for the rather unusual course and relatively rare duodenal ulcer origination at the age of 50."}
{"id": "PMID:462936", "title": "[Changes in the small intestine mucosa in liver cirrhosis].", "content": "A morphological examination was carried out of 33 patients with cirrhosis of liver and 10 clinically healthy subjects. Chronic jejunitis, with the prevalence of its atrophic forms (in 59.1%) was established to develop in 86.4 per cent of the patients. The morphological characteristics of the changes observed (prevalence of dystrophic-destructive processes with marked lesions of the specialized structures, presence of hypore-generative and moderately manifested inflammatory reaction) allow that they be considered as endogenously conditioned forms of chronic jejunitis.", "contents": "[Changes in the small intestine mucosa in liver cirrhosis]. A morphological examination was carried out of 33 patients with cirrhosis of liver and 10 clinically healthy subjects. Chronic jejunitis, with the prevalence of its atrophic forms (in 59.1%) was established to develop in 86.4 per cent of the patients. The morphological characteristics of the changes observed (prevalence of dystrophic-destructive processes with marked lesions of the specialized structures, presence of hypore-generative and moderately manifested inflammatory reaction) allow that they be considered as endogenously conditioned forms of chronic jejunitis."}
{"id": "PMID:462928", "title": "[Accelerated method of determining the antiviral activity of interferon inducers in experimental infection].", "content": "By the example of trials of two typical interferon inducers: a double-stranded complex of synthetic polyribonucleotides poly (I).poly (C) and polyacrylic acid the use of the proposed rapid method for the determination of the antiviral activity of interferon inducers in an experimental infection of mice with an alphavirus is described. The method permits to perform a statistically treated evaluation of the antiviral effect of a group of preparations within 96 hours. The mean standard error of the method is +/- 0,333 1g PFU per 1 ml of 10% brain suspension.", "contents": "[Accelerated method of determining the antiviral activity of interferon inducers in experimental infection]. By the example of trials of two typical interferon inducers: a double-stranded complex of synthetic polyribonucleotides poly (I).poly (C) and polyacrylic acid the use of the proposed rapid method for the determination of the antiviral activity of interferon inducers in an experimental infection of mice with an alphavirus is described. The method permits to perform a statistically treated evaluation of the antiviral effect of a group of preparations within 96 hours. The mean standard error of the method is +/- 0,333 1g PFU per 1 ml of 10% brain suspension."}
{"id": "PMID:462939", "title": "[Echocardiographic indices of the pumping and contractile functions of the left ventricle and left ventricular cardiac insufficiency].", "content": "EchoC-indices for the left ventricle function and the degree of left ventricular insufficiency are confronted in 148 patients with various degree of left ventricular insufficiency determined by clinical and instrumental non-invasive examination, right and/or left heart catheterization and the method of dye dilution. It was established that in the differentiation of the initial left ventricular insufficiency, EchoC-indices of the pump and contractility function of the left ventricle have a diagnostic sensitivity of 94 per cent and give a relatively low false positive (12%) and false negative results (6%). EchoC-indices, which are with the highest diagnostic sensitivity in the determination of the initial left-ventricular insufficiency, are the telediastolic size and left ventricular volume; the fraction of shortening, average rate of contraction of the circumferential fibrils and the fraction of forcing out.", "contents": "[Echocardiographic indices of the pumping and contractile functions of the left ventricle and left ventricular cardiac insufficiency]. EchoC-indices for the left ventricle function and the degree of left ventricular insufficiency are confronted in 148 patients with various degree of left ventricular insufficiency determined by clinical and instrumental non-invasive examination, right and/or left heart catheterization and the method of dye dilution. It was established that in the differentiation of the initial left ventricular insufficiency, EchoC-indices of the pump and contractility function of the left ventricle have a diagnostic sensitivity of 94 per cent and give a relatively low false positive (12%) and false negative results (6%). EchoC-indices, which are with the highest diagnostic sensitivity in the determination of the initial left-ventricular insufficiency, are the telediastolic size and left ventricular volume; the fraction of shortening, average rate of contraction of the circumferential fibrils and the fraction of forcing out."}
{"id": "PMID:462940", "title": "[Impedance cardiography. Studies on healthy subjects. Notes on methodology and its clinical importance].", "content": "The authors draw the attention to certain technical details in recording the impedance cardiography and the reading of the records, being of essential importance for the method accuracy. A new method is proposed for the measuring of (dZ/dt) min and T. Seventy healthy subjects were examined. The reliability of impedance cardiography in the determination of stroke volume (gammacardiographic controls) and the significance of the method for the dynamic follow up of cardiac and pulmonary state are discussed.", "contents": "[Impedance cardiography. Studies on healthy subjects. Notes on methodology and its clinical importance]. The authors draw the attention to certain technical details in recording the impedance cardiography and the reading of the records, being of essential importance for the method accuracy. A new method is proposed for the measuring of (dZ/dt) min and T. Seventy healthy subjects were examined. The reliability of impedance cardiography in the determination of stroke volume (gammacardiographic controls) and the significance of the method for the dynamic follow up of cardiac and pulmonary state are discussed."}
{"id": "PMID:462941", "title": "[Heart rupture as an outcome of myocardial infarct].", "content": "The authors studied the incidence and character of cardiac rupture in 109 deceased of myocardial infarction. Rupture was encountered in 21.1 per cent. Out of the 23 ruptures -- 21 were external and 2 -- internal at the intraventricular septum. Cardiac rupture is an occurence mainly in subjects over 60. Extensive transmural infarction was diagnosed in all cases of external rupture. The diagnosis was made while still alive in a little more than a half of the cases. In seven of the cases, the external rupture was with a protracted progress with the following clinical manifestations -- preceeding strong, refractory, continuous pain and anxiety, protracted shock state, prolonged pericardial friction. Characteristic ECG changes are described in case of protracted ruptures enabling the admittance of the rupture while still living. The authors express the opinion that in cases with protracted rupture, surgical life-saving intervention is possible.", "contents": "[Heart rupture as an outcome of myocardial infarct]. The authors studied the incidence and character of cardiac rupture in 109 deceased of myocardial infarction. Rupture was encountered in 21.1 per cent. Out of the 23 ruptures -- 21 were external and 2 -- internal at the intraventricular septum. Cardiac rupture is an occurence mainly in subjects over 60. Extensive transmural infarction was diagnosed in all cases of external rupture. The diagnosis was made while still alive in a little more than a half of the cases. In seven of the cases, the external rupture was with a protracted progress with the following clinical manifestations -- preceeding strong, refractory, continuous pain and anxiety, protracted shock state, prolonged pericardial friction. Characteristic ECG changes are described in case of protracted ruptures enabling the admittance of the rupture while still living. The authors express the opinion that in cases with protracted rupture, surgical life-saving intervention is possible."}
{"id": "PMID:462943", "title": "[Changes in renal function in middle and old age established by isotopic nephrography].", "content": "The authors analyse the isotope-nephrographic examinations of 112 subjects, aged over 40, their clinical, paraclinical and X-ray examinations excluding an urological ailment. In 75 (67%) of the examined, isotope-nephrography proved to be pathological. Age advancing is accompanied by an increase of the patients with isotope-nephrogram deviations. Whereas at the age of 41--50, the pathological isotope nephrograms cover only 23.1 per cent of the examined, in the age groups over 60 that per cent is four times higher. Males are 1.8 times more frequently affected as compared with the females. After the qualitative and quantitative analysis three types of pathological curves were differentiated: with disturbed vascular and secretory phase: with disturbed vascular, secretory and excretory phase and nephrectomic type. In 57.3 per cent the deviations were bilateral and in 42l7 per cent--unilateral. The disturbances of the secretory function on the background of a reduced vascularization, is most frequently encountered (65.3%).", "contents": "[Changes in renal function in middle and old age established by isotopic nephrography]. The authors analyse the isotope-nephrographic examinations of 112 subjects, aged over 40, their clinical, paraclinical and X-ray examinations excluding an urological ailment. In 75 (67%) of the examined, isotope-nephrography proved to be pathological. Age advancing is accompanied by an increase of the patients with isotope-nephrogram deviations. Whereas at the age of 41--50, the pathological isotope nephrograms cover only 23.1 per cent of the examined, in the age groups over 60 that per cent is four times higher. Males are 1.8 times more frequently affected as compared with the females. After the qualitative and quantitative analysis three types of pathological curves were differentiated: with disturbed vascular and secretory phase: with disturbed vascular, secretory and excretory phase and nephrectomic type. In 57.3 per cent the deviations were bilateral and in 42l7 per cent--unilateral. The disturbances of the secretory function on the background of a reduced vascularization, is most frequently encountered (65.3%)."}
{"id": "PMID:462944", "title": "[Embolisms and thromboses in the lesser circulation and disseminated intravascular coagulation].", "content": "Investigations were carried out on 601 deceased with embolism and thrombosis in pulmonary circulation (ETPC), in 53 of the cases (8.82%) a generalized, in two or more organs, disseminated intravasal coagulation (DIC) was found. Generalized DIC develops most often in autochthon pulmonary thrombosis (in 30.91%) and more rarely in pulmonary embolism with additional autochthon thrombformation (in 15.19%). In a great part of pulmonary embolism with additional autochthon thromb-formation (96.62%) and autochthon pulmonary thrombosis (87.27%) DIC develops locally only in pulmonary microcirculation. The local DIC in lungs is characterized morphologically by the formation of multiple fibrin microthrombus (2--3 microthrombuses per square cm section) in the microcirculatory system of lungs. The development of local or generalized DIC aggravates the course of ETPC and could play an essential role in thrombogenesis, both by mechanic occlusion of pulmonary vessels and by serotonun elimination from the destroying thrombocytes.", "contents": "[Embolisms and thromboses in the lesser circulation and disseminated intravascular coagulation]. Investigations were carried out on 601 deceased with embolism and thrombosis in pulmonary circulation (ETPC), in 53 of the cases (8.82%) a generalized, in two or more organs, disseminated intravasal coagulation (DIC) was found. Generalized DIC develops most often in autochthon pulmonary thrombosis (in 30.91%) and more rarely in pulmonary embolism with additional autochthon thrombformation (in 15.19%). In a great part of pulmonary embolism with additional autochthon thromb-formation (96.62%) and autochthon pulmonary thrombosis (87.27%) DIC develops locally only in pulmonary microcirculation. The local DIC in lungs is characterized morphologically by the formation of multiple fibrin microthrombus (2--3 microthrombuses per square cm section) in the microcirculatory system of lungs. The development of local or generalized DIC aggravates the course of ETPC and could play an essential role in thrombogenesis, both by mechanic occlusion of pulmonary vessels and by serotonun elimination from the destroying thrombocytes."}
{"id": "PMID:463037", "title": "[Differences in the evaluation of the iodine fibrinogen test (author's transl)].", "content": "The iodine fibrinogen test was evaluated using 13 different methods in 5 patients. The great variation in obtained results to assess the data makes standardization of evaluation methods imperative.", "contents": "[Differences in the evaluation of the iodine fibrinogen test (author's transl)]. The iodine fibrinogen test was evaluated using 13 different methods in 5 patients. The great variation in obtained results to assess the data makes standardization of evaluation methods imperative."}
{"id": "PMID:463038", "title": "[Facultative wound myiasis of man in Austria (author's transl)].", "content": "Three cases of contingent wound myiasis (traumatic myiasis) in man in Austria caused by Lucilia sericata (2 cases) and Sarcophaga argyrostoma and Lucilia sericata (1 case) are reported.", "contents": "[Facultative wound myiasis of man in Austria (author's transl)]. Three cases of contingent wound myiasis (traumatic myiasis) in man in Austria caused by Lucilia sericata (2 cases) and Sarcophaga argyrostoma and Lucilia sericata (1 case) are reported."}
{"id": "PMID:463039", "title": "[Operative treatment of vesico-vaginal fistulae in the University Hospital, Lom\u00e9 (Togo, West Africa) (author's transl)].", "content": "This publication draws attention to the importance of knowing about the existence of vesico-vaginal fistulae with or without disturbance of normal sphincteric control of the bladder. The repair procedure and avoidance of urinary incontinence after successful anatomical closure of the fistulae depends on the preoperative assessment of sphincteric function. A classification is presented in schematic form of the various types of vesico-vaginal fistulae with the appropriate surgical method of choice, according to our experience in this field, to obtain optimum functional results. In particular, our own operative procedure for substitution of absent sphincteric bladder control is detailed and discussed. Finally, attention is drawn to the balloon catheter test and the possibility of obtaining improvement in the surgical management of vesico-vaginal fistulae by a combination and modification of established operative procedures.", "contents": "[Operative treatment of vesico-vaginal fistulae in the University Hospital, Lom\u00e9 (Togo, West Africa) (author's transl)]. This publication draws attention to the importance of knowing about the existence of vesico-vaginal fistulae with or without disturbance of normal sphincteric control of the bladder. The repair procedure and avoidance of urinary incontinence after successful anatomical closure of the fistulae depends on the preoperative assessment of sphincteric function. A classification is presented in schematic form of the various types of vesico-vaginal fistulae with the appropriate surgical method of choice, according to our experience in this field, to obtain optimum functional results. In particular, our own operative procedure for substitution of absent sphincteric bladder control is detailed and discussed. Finally, attention is drawn to the balloon catheter test and the possibility of obtaining improvement in the surgical management of vesico-vaginal fistulae by a combination and modification of established operative procedures."}
{"id": "PMID:463040", "title": "[Standardised radiological investigation of the stomach with double contrast and fluorography (author's transl)].", "content": "The aims are presented and the technical equipment and application of different contrast media (barium sulphate and gas) are discussed. The steps of examination are divided into 5 phases. Thus, each part of the stomach is shown both by extreme filling and relief technique and by double contrast. The advantages and disadvantages of this technique are discussed. It is important to point out that the advantages of the double-contrast method could be fully realised only by fluorography.", "contents": "[Standardised radiological investigation of the stomach with double contrast and fluorography (author's transl)]. The aims are presented and the technical equipment and application of different contrast media (barium sulphate and gas) are discussed. The steps of examination are divided into 5 phases. Thus, each part of the stomach is shown both by extreme filling and relief technique and by double contrast. The advantages and disadvantages of this technique are discussed. It is important to point out that the advantages of the double-contrast method could be fully realised only by fluorography."}
{"id": "PMID:463041", "title": "[Controlled hypotension with sodium nitroprosside in patients placed in the sitting position during intracranial operations (author's transl)].", "content": "Sodium nitroprusside was used to induce hypotension during continuous neuroleptanalgesia in 11 neurosurgical patients aged between 13 and 53 years placed in the sitting position. No patient had cardiovascular disease. 14 patients aged between 18 and 72 years without induced hypotension were used as controls to compare the action of our standardized method of anaesthesia on the same parameters that were also monitored during blood pressure lowering. Cerebral perfusion of these 14 patients with highly elevated intracranial pressure with or without cardiovascular disease already seemed to be threatened by the upright position. Therefore, induced hypotension was not performed. The average dosage of sodium nitroprusside was 2.08 mcg/kg/min. The initial medium arterial pressure was lowered by a mean of 32.4%, associated with an average increase in pulse rate of 38.9%. The values of arterial PO2 never fell below 100 mmHg and the PCO2 remained between 25 and 35 mm Hg, indicating adequate cardiovascular function and gas exchange. Careful monitoring of the patients during and after blood pressure lowering with sodium nitroprusside made it possible to take advantage of the induced hypotension also in strictly-selected neurosurgical patients who have to be operated on in upright position.", "contents": "[Controlled hypotension with sodium nitroprosside in patients placed in the sitting position during intracranial operations (author's transl)]. Sodium nitroprusside was used to induce hypotension during continuous neuroleptanalgesia in 11 neurosurgical patients aged between 13 and 53 years placed in the sitting position. No patient had cardiovascular disease. 14 patients aged between 18 and 72 years without induced hypotension were used as controls to compare the action of our standardized method of anaesthesia on the same parameters that were also monitored during blood pressure lowering. Cerebral perfusion of these 14 patients with highly elevated intracranial pressure with or without cardiovascular disease already seemed to be threatened by the upright position. Therefore, induced hypotension was not performed. The average dosage of sodium nitroprusside was 2.08 mcg/kg/min. The initial medium arterial pressure was lowered by a mean of 32.4%, associated with an average increase in pulse rate of 38.9%. The values of arterial PO2 never fell below 100 mmHg and the PCO2 remained between 25 and 35 mm Hg, indicating adequate cardiovascular function and gas exchange. Careful monitoring of the patients during and after blood pressure lowering with sodium nitroprusside made it possible to take advantage of the induced hypotension also in strictly-selected neurosurgical patients who have to be operated on in upright position."}
{"id": "PMID:463042", "title": "[Animal experiments on combined local immunization against influenza (author's transl)].", "content": "Mice in ether narcosis were immunized 10 times by means of an inhalation spray with Begrivacs S (Behring-Werke) and then infected nasally with the virulent A/PR8 virus 7 to 12 days after the last immunization. A significant protection was achieved as gauged from the mortality rate. Furthermore, mice were nasally immunized with the polyvalent bacterial lysate vaccine IRS 19 (Sarbach, Chatillon) and subsequently infected nasally with the virulent influenza virus. A significant degree of non-specific protection also developed under these conditions, but was less effective than that after specific immunization. The best protective effect can be gained by applying both vaccines (Begrivac S and IRS 19) in combination. In this case, the mortality rate of the test animals decreases from 68% in the control to 31% in the animals receiving combined vaccination.", "contents": "[Animal experiments on combined local immunization against influenza (author's transl)]. Mice in ether narcosis were immunized 10 times by means of an inhalation spray with Begrivacs S (Behring-Werke) and then infected nasally with the virulent A/PR8 virus 7 to 12 days after the last immunization. A significant protection was achieved as gauged from the mortality rate. Furthermore, mice were nasally immunized with the polyvalent bacterial lysate vaccine IRS 19 (Sarbach, Chatillon) and subsequently infected nasally with the virulent influenza virus. A significant degree of non-specific protection also developed under these conditions, but was less effective than that after specific immunization. The best protective effect can be gained by applying both vaccines (Begrivac S and IRS 19) in combination. In this case, the mortality rate of the test animals decreases from 68% in the control to 31% in the animals receiving combined vaccination."}
{"id": "PMID:463043", "title": "[Oral administration of insulin by means of liposomes in animal experiments (author's transl)].", "content": "Liposomes are an effective vehicle for the oral administration of insulin. They are prepared from lipid emulsions by sonication and particles of homogeneous size are generated by elution through sepharose columns. Liposomes are taken up into the gastric mucosa by endocytosis and then transported to the liver via the portal circulation. Oral administration of 10 U insulin/kg body weight to rats is followed by a reduction in blood glucose to 67% of the initial value. When liposome-trapped insulin was injected intravenously a decrease in blood glucose to 40% of the initial value was obtained by the administration of 5 IU insulin/kg body weight. While the effect of orally-administered liposome-trapped insulin is obvious, the problems of standardization of the insulin content of the liposomes and the great variability of liposome uptake into the gastric mucosa by endocytosis remain unsolved.", "contents": "[Oral administration of insulin by means of liposomes in animal experiments (author's transl)]. Liposomes are an effective vehicle for the oral administration of insulin. They are prepared from lipid emulsions by sonication and particles of homogeneous size are generated by elution through sepharose columns. Liposomes are taken up into the gastric mucosa by endocytosis and then transported to the liver via the portal circulation. Oral administration of 10 U insulin/kg body weight to rats is followed by a reduction in blood glucose to 67% of the initial value. When liposome-trapped insulin was injected intravenously a decrease in blood glucose to 40% of the initial value was obtained by the administration of 5 IU insulin/kg body weight. While the effect of orally-administered liposome-trapped insulin is obvious, the problems of standardization of the insulin content of the liposomes and the great variability of liposome uptake into the gastric mucosa by endocytosis remain unsolved."}
{"id": "PMID:463044", "title": "[Diagnostic and epidemiological features of a coxsackie virus epidemic in newborn infants (author's transl)].", "content": "This report deals with a hospital outbreak of Coxsackie virus infections in a neonatal ward. Most infections were mild, but one boy died from severe toxicosis. Six children had an abnormal ECG. Virus was isolated from 3 children, including the fatal case. One virus was Coxsackie B5, the two others Coxsackie A9. A rise in titre in the neutralisation test with respect to Coxsackie B5 virus was found in the sera of 13 patients and to Coxsackie A9 virus in a further two cases. Four cases were diagnosed using a Coxsackie B mix antigen and 10 by means of a picorna mix antigen in the CFT. Thus the picorna mix antigen proved to be very useful in the diagnosis of Coxsackie infection in newborn infants. Coxsackie viruses were also isolated from the faeces of ward personnel and serological evidence of recent infection was found by means of the CFT. The epidemiological implications of these findings are discussed.", "contents": "[Diagnostic and epidemiological features of a coxsackie virus epidemic in newborn infants (author's transl)]. This report deals with a hospital outbreak of Coxsackie virus infections in a neonatal ward. Most infections were mild, but one boy died from severe toxicosis. Six children had an abnormal ECG. Virus was isolated from 3 children, including the fatal case. One virus was Coxsackie B5, the two others Coxsackie A9. A rise in titre in the neutralisation test with respect to Coxsackie B5 virus was found in the sera of 13 patients and to Coxsackie A9 virus in a further two cases. Four cases were diagnosed using a Coxsackie B mix antigen and 10 by means of a picorna mix antigen in the CFT. Thus the picorna mix antigen proved to be very useful in the diagnosis of Coxsackie infection in newborn infants. Coxsackie viruses were also isolated from the faeces of ward personnel and serological evidence of recent infection was found by means of the CFT. The epidemiological implications of these findings are discussed."}
{"id": "PMID:463045", "title": "[Administration of 15-(S)-15-methyl prostaglandins F2 alpha in intrauterine fetal death, missed abortion and hydatidiform mole (author's transl)].", "content": "10 patients with missed abortion, intrauterine fetal death or hydatifidiform mole were given 15-(S)-15-methyl prostaglandin F2 alpha intramuscularly for the induction of labour or, in 2 cases, to obtain softening of the cervix prior to curettage. The mean time interval between induction and abortion was 6 h 9 min, with a mean dosage of 890 mcg prostaglandin per patient. Vomiting or diarrhoea occurred in 7 patients. Apart from a drop in haemoglobin concentration in 1 patient and a temporary increase in white cell count in 6 patients, no other pathological laboratory findings were detected. We conclude from these results and the relevant literature that the intramuscular administration of 15-(S)-15-methyl prostaglandin is an effective and safe means of inducing labour in missed abortion, intrauterine fetal death and hydratidiform mole.", "contents": "[Administration of 15-(S)-15-methyl prostaglandins F2 alpha in intrauterine fetal death, missed abortion and hydatidiform mole (author's transl)]. 10 patients with missed abortion, intrauterine fetal death or hydatifidiform mole were given 15-(S)-15-methyl prostaglandin F2 alpha intramuscularly for the induction of labour or, in 2 cases, to obtain softening of the cervix prior to curettage. The mean time interval between induction and abortion was 6 h 9 min, with a mean dosage of 890 mcg prostaglandin per patient. Vomiting or diarrhoea occurred in 7 patients. Apart from a drop in haemoglobin concentration in 1 patient and a temporary increase in white cell count in 6 patients, no other pathological laboratory findings were detected. We conclude from these results and the relevant literature that the intramuscular administration of 15-(S)-15-methyl prostaglandin is an effective and safe means of inducing labour in missed abortion, intrauterine fetal death and hydratidiform mole."}
{"id": "PMID:463046", "title": "[The diagnostic accuracy of the dipyridamole test in coronary heart disease (author's transl)].", "content": "The diagnostic accuracy of the dipyridamole test in provoking coronary insufficiency was investigated in 79 patients with chest pain and the results were compared with the findings on angiography and exercise electrocardiogram. 58 patients had documented severe coronary artery stenosis, 21 had patent coronary vessels (cardiomyopathy 8, aortic stenosis 1, ectopic origin of coronary artery 1, normal 11). Anginal pain after dipyridamole was a non-specific finding. Approximately half the subjects in whom coronary insufficiency would be expected according to the coronary angiographic and ventriculographic findings evidenced ischaemic ST-segment depression after dipyridamole, which was comparable to the number of positive exercise electrocardiograms. In 23 patients, most of whom had shown an inadequate frequency response during the initial exercise test, ergometry was repeated after the administration of dipyridamole. This resulted in an increase in ischaemic ECG response from 26 to 70%. It is concluded that a stress test combining dipyridamole and submaximum exercise increases the incidence of ischaemic ST-segment depression in comparison with ergometry alone. Anginal pain without ST-segment depression proved to be without diagnostic value.", "contents": "[The diagnostic accuracy of the dipyridamole test in coronary heart disease (author's transl)]. The diagnostic accuracy of the dipyridamole test in provoking coronary insufficiency was investigated in 79 patients with chest pain and the results were compared with the findings on angiography and exercise electrocardiogram. 58 patients had documented severe coronary artery stenosis, 21 had patent coronary vessels (cardiomyopathy 8, aortic stenosis 1, ectopic origin of coronary artery 1, normal 11). Anginal pain after dipyridamole was a non-specific finding. Approximately half the subjects in whom coronary insufficiency would be expected according to the coronary angiographic and ventriculographic findings evidenced ischaemic ST-segment depression after dipyridamole, which was comparable to the number of positive exercise electrocardiograms. In 23 patients, most of whom had shown an inadequate frequency response during the initial exercise test, ergometry was repeated after the administration of dipyridamole. This resulted in an increase in ischaemic ECG response from 26 to 70%. It is concluded that a stress test combining dipyridamole and submaximum exercise increases the incidence of ischaemic ST-segment depression in comparison with ergometry alone. Anginal pain without ST-segment depression proved to be without diagnostic value."}
{"id": "PMID:463047", "title": "[Radioimmunological determination of parathyroid hormone: regional localization in hypercalcaemic hyperparathyroidism (author's transl)].", "content": "Angiographic or scintigraphic localization of parathyroid adenomas is successful in only a small number of patients with hypercalcaemic hyperparathyroidism. This report is concerned with the preoperative localization of parathyroid adenomas by regional catheterization of the thyroid veins in patients with hypercalcaemic hyperparathyroidism (n = 7). PTH was determined radioimmunologically, using an antiserum specific for PTH1-84 and the carboxyl-terminal fragment of the hormone. Determination of regional differences in the plasma concentration of PTH permitted the preoperative localization of PTH secreting adenomas. The preoperative localization of parathyroid adenomas was confirmed in each instance by surgery. Thus, the regional determination of plasma PTH represents a tool to improve the preoperative localization of parathyroid adenomas in patients with hypercalcaemic hyperparathyroidism and, hence, to reduce the risk of an unsuccessful operation.", "contents": "[Radioimmunological determination of parathyroid hormone: regional localization in hypercalcaemic hyperparathyroidism (author's transl)]. Angiographic or scintigraphic localization of parathyroid adenomas is successful in only a small number of patients with hypercalcaemic hyperparathyroidism. This report is concerned with the preoperative localization of parathyroid adenomas by regional catheterization of the thyroid veins in patients with hypercalcaemic hyperparathyroidism (n = 7). PTH was determined radioimmunologically, using an antiserum specific for PTH1-84 and the carboxyl-terminal fragment of the hormone. Determination of regional differences in the plasma concentration of PTH permitted the preoperative localization of PTH secreting adenomas. The preoperative localization of parathyroid adenomas was confirmed in each instance by surgery. Thus, the regional determination of plasma PTH represents a tool to improve the preoperative localization of parathyroid adenomas in patients with hypercalcaemic hyperparathyroidism and, hence, to reduce the risk of an unsuccessful operation."}
{"id": "PMID:463048", "title": "[Investigation of bone growth into porous metal implants (author's transl)].", "content": "Stainless-steel cylinders of different pore size were implanted into the tibia, and small tubes, 500 micrometers diameter, of the same material into the iliac crests of 19 rabbits. It was found that after three weeks ingrowing bone tissue anchored the cylinders if the pore size was adequate. The same applied to the tubes. A stable implantation was the prerequisite for the ingrowth of fibre-bone trabeculae. Additionally, the use of fibrinogen adhesive system seems to be advantageous for this anchorage. These results were established by mechanical tensile tests and morphological examinations.", "contents": "[Investigation of bone growth into porous metal implants (author's transl)]. Stainless-steel cylinders of different pore size were implanted into the tibia, and small tubes, 500 micrometers diameter, of the same material into the iliac crests of 19 rabbits. It was found that after three weeks ingrowing bone tissue anchored the cylinders if the pore size was adequate. The same applied to the tubes. A stable implantation was the prerequisite for the ingrowth of fibre-bone trabeculae. Additionally, the use of fibrinogen adhesive system seems to be advantageous for this anchorage. These results were established by mechanical tensile tests and morphological examinations."}
{"id": "PMID:463049", "title": "[The radioprotective effect of iodine and its preventive action on renal calcification (author's transl)].", "content": "The protective effect of iodine against injurious damage caused by ionizing radiation was studied in 26 rats irradiated with cobalt-60 gamma rays as a single dose of 1000 rads (10 joule/kg). Twelve rats were pretreated 13 times every second day before irradiation. After irradiation they were treated daily during 21 days with 2.5 to 3.0 mg iodine given subcutaneously along with 9 mg Ca2+ (as gluconolactobionate) intramuscularly. In addition, they received 500 micrograms of iodine in their food daily. 67% of the iodine-treated rats remained alive after 30 days in contrast to 36% of the control rats which received Ca2+ only. 10 further rats which received neither iodine nor Ca2+ died within 3 to 5 days after irradiation. Deposition of calcium in the renal parenchyma was observed in 12 out of the 14 control rats, but only in 2 out of the 12 iodine-treated rats. It can be concluded from the results that iodine has a protective effect on ionizing radiation. It is assumed that iodine promotes the energy-conserving mechanism in mitochondria and also prevents the irradiation-induced decrease in calcium efflux.", "contents": "[The radioprotective effect of iodine and its preventive action on renal calcification (author's transl)]. The protective effect of iodine against injurious damage caused by ionizing radiation was studied in 26 rats irradiated with cobalt-60 gamma rays as a single dose of 1000 rads (10 joule/kg). Twelve rats were pretreated 13 times every second day before irradiation. After irradiation they were treated daily during 21 days with 2.5 to 3.0 mg iodine given subcutaneously along with 9 mg Ca2+ (as gluconolactobionate) intramuscularly. In addition, they received 500 micrograms of iodine in their food daily. 67% of the iodine-treated rats remained alive after 30 days in contrast to 36% of the control rats which received Ca2+ only. 10 further rats which received neither iodine nor Ca2+ died within 3 to 5 days after irradiation. Deposition of calcium in the renal parenchyma was observed in 12 out of the 14 control rats, but only in 2 out of the 12 iodine-treated rats. It can be concluded from the results that iodine has a protective effect on ionizing radiation. It is assumed that iodine promotes the energy-conserving mechanism in mitochondria and also prevents the irradiation-induced decrease in calcium efflux."}
{"id": "PMID:463050", "title": "[Feedback by objective tests for coordination of medical teaching (author's transl)].", "content": "Within the framework of an experimentally introduced curriculum in Medical Physiology during the past three years, voluntary tests of the multiple-choice type were offered at the end of each term in order to provide students with feedback on their achievements. Positive results contribute to the final grade. Analysis of test results reveals improvement after the first year; results of subsequent tests are fairly stable. Selected items are presented in detail in order to show the distribution of choices. As revealed by item analysis the attractivity of distractors seems to be of a specific nature. Statistical results and interpretation of content of questions derived from different fields indicate that valuable feedback might be obtained from objective tests when interdisciplinary coordination of the curriculum is to be achieved.", "contents": "[Feedback by objective tests for coordination of medical teaching (author's transl)]. Within the framework of an experimentally introduced curriculum in Medical Physiology during the past three years, voluntary tests of the multiple-choice type were offered at the end of each term in order to provide students with feedback on their achievements. Positive results contribute to the final grade. Analysis of test results reveals improvement after the first year; results of subsequent tests are fairly stable. Selected items are presented in detail in order to show the distribution of choices. As revealed by item analysis the attractivity of distractors seems to be of a specific nature. Statistical results and interpretation of content of questions derived from different fields indicate that valuable feedback might be obtained from objective tests when interdisciplinary coordination of the curriculum is to be achieved."}
{"id": "PMID:463051", "title": "[The effect of high-heeled shoes on the venous pressure of the legs (author's transl)].", "content": "The influence of high-heeled shoes on the venous pressure in the lower limbs is studied. In a test series on an exercise band subjects with high heels showed a significantly smaller drop in pressure than subjects wearing flat shoes.", "contents": "[The effect of high-heeled shoes on the venous pressure of the legs (author's transl)]. The influence of high-heeled shoes on the venous pressure in the lower limbs is studied. In a test series on an exercise band subjects with high heels showed a significantly smaller drop in pressure than subjects wearing flat shoes."}
{"id": "PMID:463054", "title": "[Psycho-physiological effects of pop music on cardiovascular parameters at rest and during exertion (author's transl)].", "content": "The effects of pop music on heart rate, systolic and diastolic blood pressure at rest, during exercise on a bicycle ergometer and in the recovery phase were investigated. The psychological effects of the music were analysed by means of a question sheet and correlated with the physiological effects. The psychological effects of the music points to a reduction of monotony and of fatigue. During exercise, especially during low physical strain, there was a good correlation between the psychological and the physiological parameters. The significant decrease of the cardiovascular parameters in the recovery phase demonstrates, that emotional stimuli are influencing the heart rate not only at rest and work, but also in the recovery phase. The results of the psycho-physiological experiments give rise to the conclusion, that individual attitude to music doesn't primarily influence the effects of music on physiological reactions.", "contents": "[Psycho-physiological effects of pop music on cardiovascular parameters at rest and during exertion (author's transl)]. The effects of pop music on heart rate, systolic and diastolic blood pressure at rest, during exercise on a bicycle ergometer and in the recovery phase were investigated. The psychological effects of the music were analysed by means of a question sheet and correlated with the physiological effects. The psychological effects of the music points to a reduction of monotony and of fatigue. During exercise, especially during low physical strain, there was a good correlation between the psychological and the physiological parameters. The significant decrease of the cardiovascular parameters in the recovery phase demonstrates, that emotional stimuli are influencing the heart rate not only at rest and work, but also in the recovery phase. The results of the psycho-physiological experiments give rise to the conclusion, that individual attitude to music doesn't primarily influence the effects of music on physiological reactions."}
{"id": "PMID:463056", "title": "[Diagnosis of cerebral atrophy on the basis of the cerebral angiogram (author's transl)].", "content": "An increased distance between the brain surface and the skull causes the appearance of a broad avascular band in the carotid angiogram, which -- apart from other criteria -- has been valued as a sign of cerebral atrophy. 54 patients (60 to 79 years old), suffering from cerebrovascular diseases, were examined. Carotid angiography of the afflicted hemispheres were carried out within 4 to 90 days after the stroke. The initially mentioned distance between the skull and the brain surface, represented by the terminal arterial branches, was measured in frontal and lateral projection. The influence of alterations of the wall of extracranial vessels, of the number of attacks, of the severity of the stroke as well as of the patient's age on the distance was statistically evaluated. The results were compared with those of cranial computerized tomography. Only limited correlations between the mentioned parameters and the distance between the vessels and the skull have been found. Thus the distance is only of limited diagnostic value for the verification of senile cerebral atropy on a vascular basis.", "contents": "[Diagnosis of cerebral atrophy on the basis of the cerebral angiogram (author's transl)]. An increased distance between the brain surface and the skull causes the appearance of a broad avascular band in the carotid angiogram, which -- apart from other criteria -- has been valued as a sign of cerebral atrophy. 54 patients (60 to 79 years old), suffering from cerebrovascular diseases, were examined. Carotid angiography of the afflicted hemispheres were carried out within 4 to 90 days after the stroke. The initially mentioned distance between the skull and the brain surface, represented by the terminal arterial branches, was measured in frontal and lateral projection. The influence of alterations of the wall of extracranial vessels, of the number of attacks, of the severity of the stroke as well as of the patient's age on the distance was statistically evaluated. The results were compared with those of cranial computerized tomography. Only limited correlations between the mentioned parameters and the distance between the vessels and the skull have been found. Thus the distance is only of limited diagnostic value for the verification of senile cerebral atropy on a vascular basis."}
{"id": "PMID:463057", "title": "[The localization of postthrombotic changes in the different levels of the venous system of the leg (author's transl)].", "content": "In 173 legs with crural ulcers and postthrombotic syndrome proved by phlebography the frequency of involvement of the 4 levels of deep venous system was assessed. The results were compared with those obtained from a group of patients with acute or subacute deep vein thrombosis. The partly very distinct discrepancies may probably be explained by long lasting dynamics of the venous changes (spontaneous lysis, recurrent thrombosis). For the development of severe signs of venous insufficiency the involvement of the lower leg level may be the only cause in many instances.", "contents": "[The localization of postthrombotic changes in the different levels of the venous system of the leg (author's transl)]. In 173 legs with crural ulcers and postthrombotic syndrome proved by phlebography the frequency of involvement of the 4 levels of deep venous system was assessed. The results were compared with those obtained from a group of patients with acute or subacute deep vein thrombosis. The partly very distinct discrepancies may probably be explained by long lasting dynamics of the venous changes (spontaneous lysis, recurrent thrombosis). For the development of severe signs of venous insufficiency the involvement of the lower leg level may be the only cause in many instances."}
{"id": "PMID:463058", "title": "[Anorectal manometry in childhood (author's transl)].", "content": "Anorectal manometric studies with open-ended and water-filled tubes are useful in the diagnostic of many anorectal diseases. The electro-manometric investigation is together with X-ray and histologic examinations an important method in the diagnosis of Morbus Hirschsprung, especially in cases with short segment. Moreover a manometric differentiation of other forms of constipation is possible.", "contents": "[Anorectal manometry in childhood (author's transl)]. Anorectal manometric studies with open-ended and water-filled tubes are useful in the diagnostic of many anorectal diseases. The electro-manometric investigation is together with X-ray and histologic examinations an important method in the diagnosis of Morbus Hirschsprung, especially in cases with short segment. Moreover a manometric differentiation of other forms of constipation is possible."}
{"id": "PMID:463061", "title": "[Acute rupture of the interventricular septum in posterior wall infarction (author's transl)].", "content": "A rupture of the interventricular septum, as described in a case report, is found in 2% of myocardial infarctions. Clinical symptom loud systolic murmur audible at the left sternal border associated with a thrill and with signs of cardiogenic shock. The diagnosis is made by right heart catheterisation which shows a typical oxygen stepup between right atrium and right ventricle, by which the rupture of the interventricular septum can be differentiated from acute papillary muscle rupture. The therapy should be at first hemodynamic stabilisation by drugs and intraaortic balloon pump for 4 to 6 weeks and then closure of the ventricular septal defect by operation.", "contents": "[Acute rupture of the interventricular septum in posterior wall infarction (author's transl)]. A rupture of the interventricular septum, as described in a case report, is found in 2% of myocardial infarctions. Clinical symptom loud systolic murmur audible at the left sternal border associated with a thrill and with signs of cardiogenic shock. The diagnosis is made by right heart catheterisation which shows a typical oxygen stepup between right atrium and right ventricle, by which the rupture of the interventricular septum can be differentiated from acute papillary muscle rupture. The therapy should be at first hemodynamic stabilisation by drugs and intraaortic balloon pump for 4 to 6 weeks and then closure of the ventricular septal defect by operation."}
{"id": "PMID:463062", "title": "[Normal pressure hydrocephalus (author's transl)].", "content": "Report on 21 patients with the syndrome of normal pressure hydrocephalus. All patients were treated by implantation of a shunt-system (ventriculo-atrial shunt). In only 50% of the cases to result was good (complete remission of the clinical signs). The prognosis depends upon the etiology and the duration of the disease. The best results can be expected in cases of normal pressure hydrocephalus following subarachnoid hemorrhage.", "contents": "[Normal pressure hydrocephalus (author's transl)]. Report on 21 patients with the syndrome of normal pressure hydrocephalus. All patients were treated by implantation of a shunt-system (ventriculo-atrial shunt). In only 50% of the cases to result was good (complete remission of the clinical signs). The prognosis depends upon the etiology and the duration of the disease. The best results can be expected in cases of normal pressure hydrocephalus following subarachnoid hemorrhage."}
{"id": "PMID:463063", "title": "[Post-traumatic meningioma (author's transl)].", "content": "Report on a post-traumatic meningioma diagnosed 37 years after the trauma. Reference to relevant cases and the value of computed tomography for medico-legal opinion in post-traumatic sequelae.", "contents": "[Post-traumatic meningioma (author's transl)]. Report on a post-traumatic meningioma diagnosed 37 years after the trauma. Reference to relevant cases and the value of computed tomography for medico-legal opinion in post-traumatic sequelae."}
{"id": "PMID:463064", "title": "[The locked-in-syndrome (author's transl)].", "content": "On the basis of two cases some problems of the locked-in-syndrome are discussed. In most cases the cause of the syndrome is an infarct in the ventral part of the pons due to an occlusion of the vertebral or basilar artery. There is a total loss of movement exept for the movement of the eyes in all directions and the eye-closing. Although vigilance is almost not disturbed the complete loss of movement often results in a false diagnosis of cerebral coma. The EEG is an important diagnostic aid.", "contents": "[The locked-in-syndrome (author's transl)]. On the basis of two cases some problems of the locked-in-syndrome are discussed. In most cases the cause of the syndrome is an infarct in the ventral part of the pons due to an occlusion of the vertebral or basilar artery. There is a total loss of movement exept for the movement of the eyes in all directions and the eye-closing. Although vigilance is almost not disturbed the complete loss of movement often results in a false diagnosis of cerebral coma. The EEG is an important diagnostic aid."}
{"id": "PMID:463065", "title": "[Chronic thallium poisoning (author's transl)].", "content": "A rare case of criminal, chronic thallium poisoning is described. In spite of the general prohibition of the cosmetics, drugs and rodent exterminators containing thallium thallium intoxications are still observed occasionally. In the reported case typical symptoms as initial pain, dryness of the skin, constipation and insomnia were missing. The clinical picture was dominated by a polyneuropathy more pronounced in the lower extremities, a lesion of the optic nerve and the psychic symptoms of organic damage. A particular feature was the early loss of sensitivity of the anterior rami of the intercostal nerves.", "contents": "[Chronic thallium poisoning (author's transl)]. A rare case of criminal, chronic thallium poisoning is described. In spite of the general prohibition of the cosmetics, drugs and rodent exterminators containing thallium thallium intoxications are still observed occasionally. In the reported case typical symptoms as initial pain, dryness of the skin, constipation and insomnia were missing. The clinical picture was dominated by a polyneuropathy more pronounced in the lower extremities, a lesion of the optic nerve and the psychic symptoms of organic damage. A particular feature was the early loss of sensitivity of the anterior rami of the intercostal nerves."}
{"id": "PMID:463066", "title": "[Three years behaviour therapy in a general hospital (author's transl)].", "content": "On the basis of 172 cases a report is given on the experience with behaviour therapy in a general hospital. Diagnostic and therapeutic proceedings as well as the fundamentals and methods of practically applied behaviour therapy are discussed. The results indicate that the dilemma of the so-called \"healthy\" discharged patients following organic thorough examination may be rendered less harmful by this proceeding.", "contents": "[Three years behaviour therapy in a general hospital (author's transl)]. On the basis of 172 cases a report is given on the experience with behaviour therapy in a general hospital. Diagnostic and therapeutic proceedings as well as the fundamentals and methods of practically applied behaviour therapy are discussed. The results indicate that the dilemma of the so-called \"healthy\" discharged patients following organic thorough examination may be rendered less harmful by this proceeding."}
{"id": "PMID:463067", "title": "[Meningitis carcinomatosa (author's transl)].", "content": "On the basis of a case report the clinical picture of meningitis carcinomatosa is discussed. The cerebrospinal fluid is the most important criterion for the diagnosis. All other examinations (EEG, brain-scan, X-ray) yield only imperfect information. The clinical picture of meningitis carcinomatosa is similar above all to meningitis tuberculosa. If cerebrospinal fluid shows inflammatory signs and there is a breakdown of cerebral nerves (blindness, deafness) meningitis carcinomatosa always should be considered, even if thorough examination does not succeed in proving a primary tumour.", "contents": "[Meningitis carcinomatosa (author's transl)]. On the basis of a case report the clinical picture of meningitis carcinomatosa is discussed. The cerebrospinal fluid is the most important criterion for the diagnosis. All other examinations (EEG, brain-scan, X-ray) yield only imperfect information. The clinical picture of meningitis carcinomatosa is similar above all to meningitis tuberculosa. If cerebrospinal fluid shows inflammatory signs and there is a breakdown of cerebral nerves (blindness, deafness) meningitis carcinomatosa always should be considered, even if thorough examination does not succeed in proving a primary tumour."}
{"id": "PMID:463068", "title": "[Oral treatment of acute dehydration in infants by an electrolyte-glucose-solution (author's transl)].", "content": "For oral treatment of acute dehydration in infants a solution of electrolytes and glucose in water is indicated. Selfmixing of this solution is not advisable. 30 infants with dehydration caused by acute diarrhoea were treated with a granulat (Normolyt) dissolved in water resulting in a solution of appropriate composition. The solution was well accepted and well tolerated. All babies without clinical signs of shock were successfully rehydrated by the solution. No untoward effects were observed.", "contents": "[Oral treatment of acute dehydration in infants by an electrolyte-glucose-solution (author's transl)]. For oral treatment of acute dehydration in infants a solution of electrolytes and glucose in water is indicated. Selfmixing of this solution is not advisable. 30 infants with dehydration caused by acute diarrhoea were treated with a granulat (Normolyt) dissolved in water resulting in a solution of appropriate composition. The solution was well accepted and well tolerated. All babies without clinical signs of shock were successfully rehydrated by the solution. No untoward effects were observed."}
{"id": "PMID:463069", "title": "Increased production of endogenous pyrogen and lysozyme by blood monocytes in sarcoidosis.", "content": "Blood monocytes from patients with sarcoidosis were incubated in vitro, and secretion of endogenous pyrogen (EP), the protein which mediates fever, and lysozyme (L) were measured. After incubation with endotoxin, monocytes from 5 patients with sarcoidosis released twice as much EP as did monocytes from normal individuals (p < .001). Initial 24-hr secretion of L by monocytes from 6 of 11 additional patients with sarcoidosis exceeded the normal range of values for cells from 11 age- and sex-matched control individuals. Cells with initially augmented secretion rates continued to secrete increased amounts of L for 3 days. A correlation was noted between in vitro secretion of L by monocytes and serum levels of L in the same patient. These studies indicate that circulating mononuclear cells in some patients with sarcoidosis have an increased capacity to secrete EP and/or L prior to tissue localization.", "contents": "Increased production of endogenous pyrogen and lysozyme by blood monocytes in sarcoidosis. Blood monocytes from patients with sarcoidosis were incubated in vitro, and secretion of endogenous pyrogen (EP), the protein which mediates fever, and lysozyme (L) were measured. After incubation with endotoxin, monocytes from 5 patients with sarcoidosis released twice as much EP as did monocytes from normal individuals (p < .001). Initial 24-hr secretion of L by monocytes from 6 of 11 additional patients with sarcoidosis exceeded the normal range of values for cells from 11 age- and sex-matched control individuals. Cells with initially augmented secretion rates continued to secrete increased amounts of L for 3 days. A correlation was noted between in vitro secretion of L by monocytes and serum levels of L in the same patient. These studies indicate that circulating mononuclear cells in some patients with sarcoidosis have an increased capacity to secrete EP and/or L prior to tissue localization."}
{"id": "PMID:463070", "title": "Association of anemia with reduced central respiratory drive in the piglet.", "content": "Central respiratory drive was studied in 13 piglets of both sexes varying in age from 19 to 67 days. The distal trachea was cannulated and the maximum rate of isometric inspiratory pressure change (dP/dt)(max), was measured at the airway. Curves were constructed relating this measurement to changes in arterial PCO(2) during carbon dioxide rebreathing. Data were obtained at intervals corresponding to stepwise reductions in central respiratory drive produced by added chloralose anaesthesia. Laryngeal reflex activation was achieved by electrical stimulation of the superior laryngeal nerves (SLN). This caused permanent respiratory arrest at a critical level of central respiratory depression expressed as the slope of the curve relating (dP/dt)(max) to arterial PCO(2). Severely anemic piglets showed markedly decreased central respiratory drive at a given dose of anesthesia compared to controls. This was consistent with the observed greater sensitivity to laryngeal nerve stimulation in these animals. It is concluded that anemia may be associated with impaired functional maturation of central respiratory mechanisms and consequent susceptibility to laryngeal reflex apnea and asphyxial death. These observations may pertain to factors associated with the sudden infant death syndrome.", "contents": "Association of anemia with reduced central respiratory drive in the piglet. Central respiratory drive was studied in 13 piglets of both sexes varying in age from 19 to 67 days. The distal trachea was cannulated and the maximum rate of isometric inspiratory pressure change (dP/dt)(max), was measured at the airway. Curves were constructed relating this measurement to changes in arterial PCO(2) during carbon dioxide rebreathing. Data were obtained at intervals corresponding to stepwise reductions in central respiratory drive produced by added chloralose anaesthesia. Laryngeal reflex activation was achieved by electrical stimulation of the superior laryngeal nerves (SLN). This caused permanent respiratory arrest at a critical level of central respiratory depression expressed as the slope of the curve relating (dP/dt)(max) to arterial PCO(2). Severely anemic piglets showed markedly decreased central respiratory drive at a given dose of anesthesia compared to controls. This was consistent with the observed greater sensitivity to laryngeal nerve stimulation in these animals. It is concluded that anemia may be associated with impaired functional maturation of central respiratory mechanisms and consequent susceptibility to laryngeal reflex apnea and asphyxial death. These observations may pertain to factors associated with the sudden infant death syndrome."}
{"id": "PMID:463115", "title": "[BCG-vaccination in GDR (author's transl)].", "content": "Report on the development of BCG-vaccination in GDR, appreciating the initiative and the contribution of Prof. Hans Kn\u00f6ll. BCG-vaccination has a great portion of the steady reduction of tuberculosis morbidity among children and young adults and the practical disappearance of mortality in these age groups.", "contents": "[BCG-vaccination in GDR (author's transl)]. Report on the development of BCG-vaccination in GDR, appreciating the initiative and the contribution of Prof. Hans Kn\u00f6ll. BCG-vaccination has a great portion of the steady reduction of tuberculosis morbidity among children and young adults and the practical disappearance of mortality in these age groups."}
{"id": "PMID:463116", "title": "[Contribution to the spontaneous pneumothorax from the surgical point of view (author's transl)].", "content": "The number of patients with spontaneous pneumothorax has increased in the last years. Above all young asthenics, often very tall, are affected. Smokers seem to be more endangered due to their cough-attacks in the morning. Beside them also patients with traumatic or iatrogen pneumothorax were more frequently seen. The therapy of the different form of pneumothorax is becoming more and more a surgical one, thus shortening the treatment time and diminishing the rate of recidives. In the first attack thorax-drainage is preferred; the second stage of therapy in the case of recidiv-pneymothorax is the thoracotomy. The so-called \"idiopathic-spontaneous pneumothorax\" which is to be seen in young people, is very seldom.", "contents": "[Contribution to the spontaneous pneumothorax from the surgical point of view (author's transl)]. The number of patients with spontaneous pneumothorax has increased in the last years. Above all young asthenics, often very tall, are affected. Smokers seem to be more endangered due to their cough-attacks in the morning. Beside them also patients with traumatic or iatrogen pneumothorax were more frequently seen. The therapy of the different form of pneumothorax is becoming more and more a surgical one, thus shortening the treatment time and diminishing the rate of recidives. In the first attack thorax-drainage is preferred; the second stage of therapy in the case of recidiv-pneymothorax is the thoracotomy. The so-called \"idiopathic-spontaneous pneumothorax\" which is to be seen in young people, is very seldom."}
{"id": "PMID:463117", "title": "[Antibacterial activity, place of action and mechanism of resistance of pulmonal effecting antibiotics (author's transl)].", "content": "Antibiotics are of high importance in iatrogenic pathology. The emergence of resistent strains is a sanitary problem of remarkable consequences particularly under hospital conditions. Modern pulmology is not imaginable without antibiotic and chemotherapeutic drugs. A rational and economic therapy is possible only if the mechanisms and sites of antibacterial action and of drug resistance in bacteria are known.", "contents": "[Antibacterial activity, place of action and mechanism of resistance of pulmonal effecting antibiotics (author's transl)]. Antibiotics are of high importance in iatrogenic pathology. The emergence of resistent strains is a sanitary problem of remarkable consequences particularly under hospital conditions. Modern pulmology is not imaginable without antibiotic and chemotherapeutic drugs. A rational and economic therapy is possible only if the mechanisms and sites of antibacterial action and of drug resistance in bacteria are known."}
{"id": "PMID:463118", "title": "[Anatomy, diagnostics and therapy of diaphragmatic lacunae in infants and children (author's transl)].", "content": "The various forms of diaphragmatic lacunae and hernius find their explanation in the development of the diaphragm. The classification of Vos, Eijgelaar and Kuijjer should by used for typification of intraoperative findings and comparison of the therapeutic results. Own experiences are discussed concerning diagnostics, differential diagnostics and therapy.", "contents": "[Anatomy, diagnostics and therapy of diaphragmatic lacunae in infants and children (author's transl)]. The various forms of diaphragmatic lacunae and hernius find their explanation in the development of the diaphragm. The classification of Vos, Eijgelaar and Kuijjer should by used for typification of intraoperative findings and comparison of the therapeutic results. Own experiences are discussed concerning diagnostics, differential diagnostics and therapy."}
{"id": "PMID:463119", "title": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases -- an examination of about 18,000 children. 6th report: summary of the investigation in the four districts (author's transl)].", "content": "In 3 districts of the county of Halle and in the cities of Halle and Halle-Neustadt about 18,000 children of different groups of age were examined wether they were suffering from recurrent or chronic nonspecific lung diseases (CNSLD). Among the 18,000 children we found 4.3% with lung diseases. The morbidity rate was the lowest in the city of Halle and in the district of Quedlinburg, while in Bitterfeld, the district with the highest air pollution, about two times more children had fallen ill. In all districts the prevalence of CNSLD in young children was much higher than in elder ones. In the district with the highest air pollution with increasing age of the children the rate of prevalence lowered more slowly than in the other districts.", "contents": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases -- an examination of about 18,000 children. 6th report: summary of the investigation in the four districts (author's transl)]. In 3 districts of the county of Halle and in the cities of Halle and Halle-Neustadt about 18,000 children of different groups of age were examined wether they were suffering from recurrent or chronic nonspecific lung diseases (CNSLD). Among the 18,000 children we found 4.3% with lung diseases. The morbidity rate was the lowest in the city of Halle and in the district of Quedlinburg, while in Bitterfeld, the district with the highest air pollution, about two times more children had fallen ill. In all districts the prevalence of CNSLD in young children was much higher than in elder ones. In the district with the highest air pollution with increasing age of the children the rate of prevalence lowered more slowly than in the other districts."}
{"id": "PMID:463120", "title": "Studies on smoking, chronic nonspecific respiratory diseases, and lung cancer in selected territories of Czechoslovakia.", "content": "Results of epidemiological studies in selected territories of Czechoslovakia confirmed the important role of cigarette smoking as a risk factor of chronic bronchitis and lung cancer. Surveys in Kolin-District, in Prague, and Zatec revealed that the prevalence of chronic bronchitis for adult male smokers was 3--4 times, for adult female smokers twice higher than for the nonsmokers of the respective age and sex. The risk of developing lung cancer in male smokers aged 40--64 years who had smoked a total amount of 200,000 cigarettes and over was approximately 50 times higher than for nonsmokers. The smoking related respiratory diseases represent a serious medical, social, and economic problem. Their prevention carried out by various approaches, including effective smoking control measures, is an important task.", "contents": "Studies on smoking, chronic nonspecific respiratory diseases, and lung cancer in selected territories of Czechoslovakia. Results of epidemiological studies in selected territories of Czechoslovakia confirmed the important role of cigarette smoking as a risk factor of chronic bronchitis and lung cancer. Surveys in Kolin-District, in Prague, and Zatec revealed that the prevalence of chronic bronchitis for adult male smokers was 3--4 times, for adult female smokers twice higher than for the nonsmokers of the respective age and sex. The risk of developing lung cancer in male smokers aged 40--64 years who had smoked a total amount of 200,000 cigarettes and over was approximately 50 times higher than for nonsmokers. The smoking related respiratory diseases represent a serious medical, social, and economic problem. Their prevention carried out by various approaches, including effective smoking control measures, is an important task."}
{"id": "PMID:463156", "title": "[The clinical value of unspecific humoral immune parameters. Attempt at an immunogram. 1. Quantitative determination of IgG, IgM, IgA and IgD and demonstration of antibodies against cell and tissue bound antigens in patients with chronic pyelonephritis].", "content": "In the serum of patients with chronic pyelonephritis IgG, IgM, IgA, IgD and autoantibodies against organ- and species-specific renal antigens were determined. Clinical questions concerned relations to the activity of the disease and the renal function. The Ig-mean-values lying within the biological norm could be precised by taking into consideration individual biological boundary titres and relative ratio values. Pictures of tendency led to the estimation that increases of IgG and IgD appear in severely restricted renal function in normal or decreased IgM. Increases may be a criterion of activity in diseases with normal or slightly restricted renal function. IgA determinations remained without any evidence.-- Autoantibodies were found in 32,5%, increased titres were a sign of deteriorated renal function. The apparently given possibility of a humoral immunogramme for the chronic pyelonephritis is discussed, in which case a combination with other test parameters seems to be necessary.", "contents": "[The clinical value of unspecific humoral immune parameters. Attempt at an immunogram. 1. Quantitative determination of IgG, IgM, IgA and IgD and demonstration of antibodies against cell and tissue bound antigens in patients with chronic pyelonephritis]. In the serum of patients with chronic pyelonephritis IgG, IgM, IgA, IgD and autoantibodies against organ- and species-specific renal antigens were determined. Clinical questions concerned relations to the activity of the disease and the renal function. The Ig-mean-values lying within the biological norm could be precised by taking into consideration individual biological boundary titres and relative ratio values. Pictures of tendency led to the estimation that increases of IgG and IgD appear in severely restricted renal function in normal or decreased IgM. Increases may be a criterion of activity in diseases with normal or slightly restricted renal function. IgA determinations remained without any evidence.-- Autoantibodies were found in 32,5%, increased titres were a sign of deteriorated renal function. The apparently given possibility of a humoral immunogramme for the chronic pyelonephritis is discussed, in which case a combination with other test parameters seems to be necessary."}
{"id": "PMID:463157", "title": "[Complications during long-term control of the pressure in the pulmonary artery].", "content": "It is reported on two rare complications occurring in the pulmonary catheterisation with long-term control of the pressure of the pulmonary artery. In case 1 in confluenting bronchopneumonias the formation of an abscess in the region of the point of the catheter developed, in which case by the infusion therapy with very peripheral position of the catheter local lesions are very probable. In case 2 the catheter via an atrial septum defect passed into a pulmonary vein in PCA-position. A haemorrhagic infarction of the lung developed. In the two cases after removal of the catheter and adequate therapy the pulmonary complications healed.", "contents": "[Complications during long-term control of the pressure in the pulmonary artery]. It is reported on two rare complications occurring in the pulmonary catheterisation with long-term control of the pressure of the pulmonary artery. In case 1 in confluenting bronchopneumonias the formation of an abscess in the region of the point of the catheter developed, in which case by the infusion therapy with very peripheral position of the catheter local lesions are very probable. In case 2 the catheter via an atrial septum defect passed into a pulmonary vein in PCA-position. A haemorrhagic infarction of the lung developed. In the two cases after removal of the catheter and adequate therapy the pulmonary complications healed."}
{"id": "PMID:463158", "title": "[Bronchial carcinoma in multiple bilateral amyloid tumors of the lung].", "content": "It is reported on an up to now not described combination of bronchial carcinoma and multiple amyloid tumours of the lung as autopic side findings in a 68-year-old patient.", "contents": "[Bronchial carcinoma in multiple bilateral amyloid tumors of the lung]. It is reported on an up to now not described combination of bronchial carcinoma and multiple amyloid tumours of the lung as autopic side findings in a 68-year-old patient."}
{"id": "PMID:463159", "title": "[Respiratory tract diseases, their health-political importance and trends].", "content": "The diseases of the respiratory organs are defined and classified according to the 8th revision of the international classification of the diseases. The acute forms are above all represented by the infections of the respiratory tract. They are the problem No. 1 in health politics, since the most frequent exemptions from work are given on their account. A transition into chronic diseases of the respiratory tract is not infrequent. Among the causes of death the diseases of the respiratory system occupy the 3rd place, the chronic forms are an essential cause of early invalidity. Among the chronic diseases of the respiratory organs considerable shifts to the unspecific side took place. The tuberculosis is vastly reduced. The methods practised in its combat and the graduated, well functioning system of specialised outpatient institutions and hospitals proved as a very suitable instrumentarium also for the combat against the unspecific diseases of the respiratory organs. Three main groups increasingly demand our attention: bronchial carcinoma, chronic obstructive lung disease and the professionally conditioned diseases of the respiratory organs. Their combat demands a broad partnership with the physicians of the \"first line\", the factory health service and the internists. For this purpose the description of the own possibilities is necessary.", "contents": "[Respiratory tract diseases, their health-political importance and trends]. The diseases of the respiratory organs are defined and classified according to the 8th revision of the international classification of the diseases. The acute forms are above all represented by the infections of the respiratory tract. They are the problem No. 1 in health politics, since the most frequent exemptions from work are given on their account. A transition into chronic diseases of the respiratory tract is not infrequent. Among the causes of death the diseases of the respiratory system occupy the 3rd place, the chronic forms are an essential cause of early invalidity. Among the chronic diseases of the respiratory organs considerable shifts to the unspecific side took place. The tuberculosis is vastly reduced. The methods practised in its combat and the graduated, well functioning system of specialised outpatient institutions and hospitals proved as a very suitable instrumentarium also for the combat against the unspecific diseases of the respiratory organs. Three main groups increasingly demand our attention: bronchial carcinoma, chronic obstructive lung disease and the professionally conditioned diseases of the respiratory organs. Their combat demands a broad partnership with the physicians of the \"first line\", the factory health service and the internists. For this purpose the description of the own possibilities is necessary."}
{"id": "PMID:463160", "title": "[Chronic diseases of the respiratory tract (proceedings)].", "content": "On account of their increasing social-medical importance chronic diseases of the respiratory tract demand systematic measures for prevention and combat. The size of the problem is described on the basis of the results of epidemiologic investigations. Issuing from the risk degrees of the cardio-respiratory insufficiency (obstruction, hypoxaemia, pulmonary hypertension) care groups are proposed. The combat against tobacco smoking (primary prevention) and long-term treatment of the chronic obstructive bronchitis (tertiary prevention) stand in the foreground of the measures. The solution of this task demands a close cooperation with the basic medical care.", "contents": "[Chronic diseases of the respiratory tract (proceedings)]. On account of their increasing social-medical importance chronic diseases of the respiratory tract demand systematic measures for prevention and combat. The size of the problem is described on the basis of the results of epidemiologic investigations. Issuing from the risk degrees of the cardio-respiratory insufficiency (obstruction, hypoxaemia, pulmonary hypertension) care groups are proposed. The combat against tobacco smoking (primary prevention) and long-term treatment of the chronic obstructive bronchitis (tertiary prevention) stand in the foreground of the measures. The solution of this task demands a close cooperation with the basic medical care."}
{"id": "PMID:463161", "title": "[The bronchial carcinoma].", "content": "It is referred to the importance of the nicotine abuse as the evoking factor for the origination of bronchial carcinoma and to the necessity of an activation of the combat against the addiction to cigarettes, when the numbers of diseases shall be decreased. The at present still only possibility of therapy with curative character is the surgical intervention. For this a well-timed ascertainment of the diagnosis is necessary. This is successful in the peripheral circular focus carcinoma by the mass X-ray examinations. However, the to-day's 2-year mode of performance shows a decrease of the resection rate in comparison with former years. Also the carcinoma which develops in the central bronchial parts is to be found in good time, when the necessary critical attention is paid to the first of all unspecific symptomatology induced by this. With the help of instances references are given to the practicing internist.", "contents": "[The bronchial carcinoma]. It is referred to the importance of the nicotine abuse as the evoking factor for the origination of bronchial carcinoma and to the necessity of an activation of the combat against the addiction to cigarettes, when the numbers of diseases shall be decreased. The at present still only possibility of therapy with curative character is the surgical intervention. For this a well-timed ascertainment of the diagnosis is necessary. This is successful in the peripheral circular focus carcinoma by the mass X-ray examinations. However, the to-day's 2-year mode of performance shows a decrease of the resection rate in comparison with former years. Also the carcinoma which develops in the central bronchial parts is to be found in good time, when the necessary critical attention is paid to the first of all unspecific symptomatology induced by this. With the help of instances references are given to the practicing internist."}
{"id": "PMID:463164", "title": "[Therapy of tuberculosis].", "content": "The accomplishment of the principles for the prevention of a germ resistance and the development of new principles of treatment and highly effective antituberculotics has led to the fact that nowadays actually each case of tuberculosis may be treated and cured purely internally. This progress in the individual treatment were achieved by recommendations of therapy which are actualized from time to time. The modern treatment consists of a daily application of three effective antituberculotics for two to three months, fellowed by an intermittent application twice a week. The duration of the initial intensive period and the whole time of therapy is determined by the clinical and social state before the beginning and by the combination of drugs. INH, rifampizine, streptomycin and ethambutol form the components of the therapy combinations which at present are to be used no longer than twelve months. The redetection of the special microbiological qualities of the pyrazineamide and their importance for the prevention of relapses introduces the last phase of the development of the therapy of tuberculosis. By its means it is possible to shorten the duration of the treatment to 9 months and less and thus to increase the acceptability and cooperation, to decrease the toxicity and costs.", "contents": "[Therapy of tuberculosis]. The accomplishment of the principles for the prevention of a germ resistance and the development of new principles of treatment and highly effective antituberculotics has led to the fact that nowadays actually each case of tuberculosis may be treated and cured purely internally. This progress in the individual treatment were achieved by recommendations of therapy which are actualized from time to time. The modern treatment consists of a daily application of three effective antituberculotics for two to three months, fellowed by an intermittent application twice a week. The duration of the initial intensive period and the whole time of therapy is determined by the clinical and social state before the beginning and by the combination of drugs. INH, rifampizine, streptomycin and ethambutol form the components of the therapy combinations which at present are to be used no longer than twelve months. The redetection of the special microbiological qualities of the pyrazineamide and their importance for the prevention of relapses introduces the last phase of the development of the therapy of tuberculosis. By its means it is possible to shorten the duration of the treatment to 9 months and less and thus to increase the acceptability and cooperation, to decrease the toxicity and costs."}
{"id": "PMID:463166", "title": "[Case contribution to the question of side effects in the dipyridamol test].", "content": "Report on a case with disturbance of the cardiac rhythm (atrioventricular dissociation with ventricular extrasystoles) in connection with a dipyridamol test which regressed under therapy with euphyllin. This observation supports the demand of a continuous control of the ECG when the test is performed.", "contents": "[Case contribution to the question of side effects in the dipyridamol test]. Report on a case with disturbance of the cardiac rhythm (atrioventricular dissociation with ventricular extrasystoles) in connection with a dipyridamol test which regressed under therapy with euphyllin. This observation supports the demand of a continuous control of the ECG when the test is performed."}
{"id": "PMID:463167", "title": "[Results of experimental studies of 51chromium-albumin kinetics following intravenous administration of Hylase \"Dessau\"].", "content": "Experiments on the effect of Hylase on albumin kinetics were carried out by means of 51chromium-labelled albumin on 52 wistar rats. Most of the examined tissues showed a faster exchange between blood and extravascular space by help of Hylase. That was recognized from advanced peaks of activity, their higher absolute values, and partly from the faster decrease of activity in organs and tissues. This effect did not depend on the structure of capillaries, it was observed also in bradytrophic tissues (cartilage).", "contents": "[Results of experimental studies of 51chromium-albumin kinetics following intravenous administration of Hylase \"Dessau\"]. Experiments on the effect of Hylase on albumin kinetics were carried out by means of 51chromium-labelled albumin on 52 wistar rats. Most of the examined tissues showed a faster exchange between blood and extravascular space by help of Hylase. That was recognized from advanced peaks of activity, their higher absolute values, and partly from the faster decrease of activity in organs and tissues. This effect did not depend on the structure of capillaries, it was observed also in bradytrophic tissues (cartilage)."}
{"id": "PMID:463168", "title": "[Experimental animal studies of spring osteosynthesis].", "content": "Clinical experiences from spring osteosynthesis suggest that more stability and possibly earlier neogenesis of the bone in the fracture area can be achieved by suitable springs for osteosynthesis. This stronger stability of fixation could be proved in experiments on cadaveric bones. In 13 experiments on dogs the biologic occurrences at spring osteosynthesis were compared to those at inflexible osteosynthesis. Observation was carried out by means of x-ray films, Tc-scintigraphy, and histologic examination; used were the methods of applying an autologous graft and of transverse osteotomy of the femora of the dogs. The question initially asked could not be answered definitely. Basing on the reported experiences, however, an experimental procedure is proposed which might yield results with clinical relevance.", "contents": "[Experimental animal studies of spring osteosynthesis]. Clinical experiences from spring osteosynthesis suggest that more stability and possibly earlier neogenesis of the bone in the fracture area can be achieved by suitable springs for osteosynthesis. This stronger stability of fixation could be proved in experiments on cadaveric bones. In 13 experiments on dogs the biologic occurrences at spring osteosynthesis were compared to those at inflexible osteosynthesis. Observation was carried out by means of x-ray films, Tc-scintigraphy, and histologic examination; used were the methods of applying an autologous graft and of transverse osteotomy of the femora of the dogs. The question initially asked could not be answered definitely. Basing on the reported experiences, however, an experimental procedure is proposed which might yield results with clinical relevance."}
{"id": "PMID:463169", "title": "[Therapeutic endoscopy of an Abrikosov-tumor of the esophagus].", "content": "The rare and almost benign granular cell tumour has been described at first by Abrikossoff in 1925. The occurrence of this tumour in the esophagus is a rarity.--On occasion of gastroesophagoscopy of a 33-years old man we found an almost pea-sized polypous esophageal granular cell tumour, wich was completely removed with the diathermic electrosurgical snare. The endoscopic-therapeutic possibilities and limits concerning polypous lesions of the esophagus will be discussed.", "contents": "[Therapeutic endoscopy of an Abrikosov-tumor of the esophagus]. The rare and almost benign granular cell tumour has been described at first by Abrikossoff in 1925. The occurrence of this tumour in the esophagus is a rarity.--On occasion of gastroesophagoscopy of a 33-years old man we found an almost pea-sized polypous esophageal granular cell tumour, wich was completely removed with the diathermic electrosurgical snare. The endoscopic-therapeutic possibilities and limits concerning polypous lesions of the esophagus will be discussed."}
{"id": "PMID:463170", "title": "[Frequency and pathogenesis of epitheloid cell granuloma in Crohn's disease (author's transl)].", "content": "12% of the rectal biopsies (19% of the patients) showed epitheloid cell granulomas in clinically definite Crohn's disease. In colonic biopsies such granulomata were visible in 14% (22% of the patients). Granulomata were scarcely seen in normal or slightly inflamed mucosa. They were mostly found together with crypt abscesses and/or erosions and ulcers. The combination of local inflammation around crypts and crypt abscesses is rather characteristic for Crohn's disease. The inflammatory colonic alterations, visible in crohon's disease, may have the following pathogenetic sequence: Local colitis-crypt abscess--destruction of the crypt with proliferation of enlarged histiocytes--transformation histiocytes to epitheloid cells and Langhans cells--epitheloid cell granuloma.", "contents": "[Frequency and pathogenesis of epitheloid cell granuloma in Crohn's disease (author's transl)]. 12% of the rectal biopsies (19% of the patients) showed epitheloid cell granulomas in clinically definite Crohn's disease. In colonic biopsies such granulomata were visible in 14% (22% of the patients). Granulomata were scarcely seen in normal or slightly inflamed mucosa. They were mostly found together with crypt abscesses and/or erosions and ulcers. The combination of local inflammation around crypts and crypt abscesses is rather characteristic for Crohn's disease. The inflammatory colonic alterations, visible in crohon's disease, may have the following pathogenetic sequence: Local colitis-crypt abscess--destruction of the crypt with proliferation of enlarged histiocytes--transformation histiocytes to epitheloid cells and Langhans cells--epitheloid cell granuloma."}
{"id": "PMID:463171", "title": "[Occurrence of HBs-antigen and anti-HBs in patients with porphyria cutanea tarda (author's transl)].", "content": "Serum 63 patients with porphyria cutanea tarda (PCT) was examined for the presence of HBs-antigen and anti-HBs by the CombRIA-Au method. HBs-antigen was not detected in any of the examined blood samples. In contrast to these findings in 12 of the 63 PCT-patients (= 28 per cent), anti HBs was detected in the serum. The results showed that a high percentage of our PCT patients had been afflicted with an inapparent hepatitis B and were producing HBs-antibodies.", "contents": "[Occurrence of HBs-antigen and anti-HBs in patients with porphyria cutanea tarda (author's transl)]. Serum 63 patients with porphyria cutanea tarda (PCT) was examined for the presence of HBs-antigen and anti-HBs by the CombRIA-Au method. HBs-antigen was not detected in any of the examined blood samples. In contrast to these findings in 12 of the 63 PCT-patients (= 28 per cent), anti HBs was detected in the serum. The results showed that a high percentage of our PCT patients had been afflicted with an inapparent hepatitis B and were producing HBs-antibodies."}
{"id": "PMID:463172", "title": "[Exocrine pancreatic secretion, gastrin and insulin in men by intraduodenal bolus injection of calcium (author's transl)].", "content": "The possible role of calcium in human bile during the biliary stimulated exocrine pancreatic secretion was investigated in 15 healthy volunteers. Total outputs of trypsin, bicarbonate, bilirubin and volume in the duodenal juice and serum gastrin were measured during a continuous intravenous infusion of secretion (0.5 CHR U/kg/h) for 40 min. The same parameters were determined after a single intraduodenal dose of Ca++ (20 ml 13,5, 135 or 270 mval/l, n = 5 for each dose) and compared with aequivalent intraduodenal dose of Na+ and an intravenous dose of secretion/cholecystokinin (1 CHR and IDU U/kg). Low calcium (13,5 mval/l) had no effect on the output of pancreatic enzymes and bile. However the higher doses led to a significant increases of the outputs of trypsin and bilirubin, which was about 75% of the enhancement seen with secretin/cholecystokinin in the dose used. Serumgastrin secretion was significantly increased only after the higher calcium doses.--Serum insulin in peripheral venous blood venous blood was unchanged after duodenal application of 20 ml 270 mval/l calcium (n = 5). From these data one has to conclude that the Ca++-content of bile has no stimulatory effect on the exocrine pancreas and on serum gastrin and insulin.", "contents": "[Exocrine pancreatic secretion, gastrin and insulin in men by intraduodenal bolus injection of calcium (author's transl)]. The possible role of calcium in human bile during the biliary stimulated exocrine pancreatic secretion was investigated in 15 healthy volunteers. Total outputs of trypsin, bicarbonate, bilirubin and volume in the duodenal juice and serum gastrin were measured during a continuous intravenous infusion of secretion (0.5 CHR U/kg/h) for 40 min. The same parameters were determined after a single intraduodenal dose of Ca++ (20 ml 13,5, 135 or 270 mval/l, n = 5 for each dose) and compared with aequivalent intraduodenal dose of Na+ and an intravenous dose of secretion/cholecystokinin (1 CHR and IDU U/kg). Low calcium (13,5 mval/l) had no effect on the output of pancreatic enzymes and bile. However the higher doses led to a significant increases of the outputs of trypsin and bilirubin, which was about 75% of the enhancement seen with secretin/cholecystokinin in the dose used. Serumgastrin secretion was significantly increased only after the higher calcium doses.--Serum insulin in peripheral venous blood venous blood was unchanged after duodenal application of 20 ml 270 mval/l calcium (n = 5). From these data one has to conclude that the Ca++-content of bile has no stimulatory effect on the exocrine pancreas and on serum gastrin and insulin."}
{"id": "PMID:463173", "title": "[The clinical picture of \"circumscribed granuloma diseases\"].", "content": "Characteristic findings, differential diagnosis and clinical course of the so-called \"circumscript granulomas\" (Granuloma anulare, necrobiosis lipoidica, Miescher's granulomatosis, disciformis, necrobiosis maculosa, granuloma multiforme, actinic granuloma, granuloma faciale and lethal midline granuloma etc.) are represented in a condensed clinical survey.", "contents": "[The clinical picture of \"circumscribed granuloma diseases\"]. Characteristic findings, differential diagnosis and clinical course of the so-called \"circumscript granulomas\" (Granuloma anulare, necrobiosis lipoidica, Miescher's granulomatosis, disciformis, necrobiosis maculosa, granuloma multiforme, actinic granuloma, granuloma faciale and lethal midline granuloma etc.) are represented in a condensed clinical survey."}
{"id": "PMID:463178", "title": "[Congenital, self-healing reticulohistiocytosis].", "content": "The described skin condition is characterized by multiple congenital skin tumours and disseminated papules on the trunk, which regressed without therapy during the first two months of life. Apart from the skin, no other organs were involved. The histological examination showed a histiocytic infiltrate composed of cells with large, irregularly formed nuclei and partly opaque, foamy cytoplasm. Extravasation of erythrocytes was frequent. The skin disorder is similar to the congenital self-healing reticulohistocytosis of Hashimoto and Pritzker.", "contents": "[Congenital, self-healing reticulohistiocytosis]. The described skin condition is characterized by multiple congenital skin tumours and disseminated papules on the trunk, which regressed without therapy during the first two months of life. Apart from the skin, no other organs were involved. The histological examination showed a histiocytic infiltrate composed of cells with large, irregularly formed nuclei and partly opaque, foamy cytoplasm. Extravasation of erythrocytes was frequent. The skin disorder is similar to the congenital self-healing reticulohistocytosis of Hashimoto and Pritzker."}
{"id": "PMID:463181", "title": "Zinc metabolism in normal, lectin-stimulated and leukemic lymphocytes.", "content": "Gel filtration of the cytoplasmic fraction of homogenate from human peripheral lymphocytes incubated with 65Zn gave five separate peaks with molecular weights of greater than 150,000, 90,000, 65,000, 20,000, and less than 1000 daltons. Peaks I--IV consisted of proteins. The low molecular weight (peak V) may consist of nucleotides, polyamines and amino acids. After gel filtration 75-80% of the 65Zn activity was found in peak V. Lectin-induced stimulation of normal lymphocytes revealed a distribution pattern of 65Zn binding similar to that of unstimulated cells. There was only a slightly enhanced incorporation into the protein peaks I--IV. When peripheral lymphocytes of B lymphocyte origin from patients with chronic lymphocytic leukemia were incubated with 65Zn the same peaks were seen as with supernatants obtained from normal lymphocytes. Lectin-induced stimulation of leukemic lymphocytes had no significant effect on the 65Zn distribution pattern.", "contents": "Zinc metabolism in normal, lectin-stimulated and leukemic lymphocytes. Gel filtration of the cytoplasmic fraction of homogenate from human peripheral lymphocytes incubated with 65Zn gave five separate peaks with molecular weights of greater than 150,000, 90,000, 65,000, 20,000, and less than 1000 daltons. Peaks I--IV consisted of proteins. The low molecular weight (peak V) may consist of nucleotides, polyamines and amino acids. After gel filtration 75-80% of the 65Zn activity was found in peak V. Lectin-induced stimulation of normal lymphocytes revealed a distribution pattern of 65Zn binding similar to that of unstimulated cells. There was only a slightly enhanced incorporation into the protein peaks I--IV. When peripheral lymphocytes of B lymphocyte origin from patients with chronic lymphocytic leukemia were incubated with 65Zn the same peaks were seen as with supernatants obtained from normal lymphocytes. Lectin-induced stimulation of leukemic lymphocytes had no significant effect on the 65Zn distribution pattern."}
{"id": "PMID:463182", "title": "75Se-release: a short and long term assay system for cellular cytoxicity.", "content": "The gamma-emitting aminoacid 75Se-selenomethionine (75SeM) was examined as a target cell label in cytotoxic assays. It was efficiently taken up by activated, intensively metabolizing cells of various types but hardly at all by resting or low-metabolizing cells. Culturing activated cells in methionine-deficient medium with 3--5 mu Ci75SeM/ml for 18--22 h usually resulted in an uptake of 3--20 cpm/cell which was 3--200 times that of 51Cr marked cells. 75SeM-labelled cells kept in medium at ambient temperature or at 37 degrees C, maintained a high radioactivity per cell and a viability above 85% for at least 72 h without significant increase in spontaneous isotope release or loss in sensitivity in subsequent cytotoxic tests. 75Se-labelled material released from target cells was not reutilized by unlabelled lymphoid cells. Provided the cells were carefully washed after labelling and kept in optimal culture conditions, the reasonably low baseline release (usually 0.6--1.8% of input/h) in the medium control allowed performance of long-term assays of up to 54 h. However, strong cytotoxic reactions (e.g. ADCC) could cause over 50% specific 75Se-release within 5 h. With constant amounts of effector cells (3.6 x 10(3) up to 3 x 10(5)/well) identical or even higher, specific releases were obtained on 6 x 10(2) targets as compared to 1 x 10(4) targets/well. Thus, the 75Se-release assay offers a single monitoring system suitable for short (3--6 h) and long term (usually up to 44 h) cytotoxic reactions on a microscale, using 1 x 10(3) or less targets/well. Its sensitivity permits evaluation of strong and weak reactions as well as early and delayed onset cytotoxicity. In addition, with a gamma-spectrometer the radioactivity of 75Se can easily be distinguished from that of 51Cr. Due to this, and an improved method for 51Cr labelling of cells (10 mu Ci 51Cr/ml medium for 18--22 h), a double gamma-labelling of cellular proteins is available which provides new possibilities for monitoring cellular interactions in short and long term tests.", "contents": "75Se-release: a short and long term assay system for cellular cytoxicity. The gamma-emitting aminoacid 75Se-selenomethionine (75SeM) was examined as a target cell label in cytotoxic assays. It was efficiently taken up by activated, intensively metabolizing cells of various types but hardly at all by resting or low-metabolizing cells. Culturing activated cells in methionine-deficient medium with 3--5 mu Ci75SeM/ml for 18--22 h usually resulted in an uptake of 3--20 cpm/cell which was 3--200 times that of 51Cr marked cells. 75SeM-labelled cells kept in medium at ambient temperature or at 37 degrees C, maintained a high radioactivity per cell and a viability above 85% for at least 72 h without significant increase in spontaneous isotope release or loss in sensitivity in subsequent cytotoxic tests. 75Se-labelled material released from target cells was not reutilized by unlabelled lymphoid cells. Provided the cells were carefully washed after labelling and kept in optimal culture conditions, the reasonably low baseline release (usually 0.6--1.8% of input/h) in the medium control allowed performance of long-term assays of up to 54 h. However, strong cytotoxic reactions (e.g. ADCC) could cause over 50% specific 75Se-release within 5 h. With constant amounts of effector cells (3.6 x 10(3) up to 3 x 10(5)/well) identical or even higher, specific releases were obtained on 6 x 10(2) targets as compared to 1 x 10(4) targets/well. Thus, the 75Se-release assay offers a single monitoring system suitable for short (3--6 h) and long term (usually up to 44 h) cytotoxic reactions on a microscale, using 1 x 10(3) or less targets/well. Its sensitivity permits evaluation of strong and weak reactions as well as early and delayed onset cytotoxicity. In addition, with a gamma-spectrometer the radioactivity of 75Se can easily be distinguished from that of 51Cr. Due to this, and an improved method for 51Cr labelling of cells (10 mu Ci 51Cr/ml medium for 18--22 h), a double gamma-labelling of cellular proteins is available which provides new possibilities for monitoring cellular interactions in short and long term tests."}
{"id": "PMID:463183", "title": "Erythrocyte adherence to the marginal zone of mouse spleen follicle mediated by receptor(s) for neuraminic acid.", "content": "Erythrocytes of different species (chicken, sheep, man, mouse, rat, guinea pig) except rabbit erythrocytes strongly adhere to the marginal zone of mouse spleen follicles in frozen sections. This adherence reaction (AR) is not restricted to red blood cells but is also observed with human lymphocytes. Pretreatment of the tissue sections with trypsin, mercaptoethanol, periodate, chloroform/methanol, acetone, and heating the sections abolishes AR whereas neuraminidase (VCN) treatment of the sections has an amplifying effect. AR is inhibited by preincubation of the neuraminidase- or untreated sections with neuraminic acid (NA). Treatment of the erythrocytes with VCN completely abolishes AR whereas treatment with other enzymes (hyaluronidase, collagenase) is ineffective in this respect. Determination of NA in the erythrocyte membrane before and after VCN treatment reveals a positive correlation between the amount of NA and AR. Rabbit red blood cells have the lowest NA content in their membranes and, in addition, there is little effect of VCN treatment in further reducing it. It is possible that a lectin-like substance is responsible for AR. The biologic significance of AR is hypothetical, but since AR occurs in an area of the spleen playing a role in antigen trapping it is conceivable that this trapping may be mediated by interaction of NA and NA receptor(s).", "contents": "Erythrocyte adherence to the marginal zone of mouse spleen follicle mediated by receptor(s) for neuraminic acid. Erythrocytes of different species (chicken, sheep, man, mouse, rat, guinea pig) except rabbit erythrocytes strongly adhere to the marginal zone of mouse spleen follicles in frozen sections. This adherence reaction (AR) is not restricted to red blood cells but is also observed with human lymphocytes. Pretreatment of the tissue sections with trypsin, mercaptoethanol, periodate, chloroform/methanol, acetone, and heating the sections abolishes AR whereas neuraminidase (VCN) treatment of the sections has an amplifying effect. AR is inhibited by preincubation of the neuraminidase- or untreated sections with neuraminic acid (NA). Treatment of the erythrocytes with VCN completely abolishes AR whereas treatment with other enzymes (hyaluronidase, collagenase) is ineffective in this respect. Determination of NA in the erythrocyte membrane before and after VCN treatment reveals a positive correlation between the amount of NA and AR. Rabbit red blood cells have the lowest NA content in their membranes and, in addition, there is little effect of VCN treatment in further reducing it. It is possible that a lectin-like substance is responsible for AR. The biologic significance of AR is hypothetical, but since AR occurs in an area of the spleen playing a role in antigen trapping it is conceivable that this trapping may be mediated by interaction of NA and NA receptor(s)."}
{"id": "PMID:463186", "title": "[Preliminary experience with the catheter dilatation of coronary artery stenoses (author's transl)].", "content": "The method of percutaneous transluminal coronary angioplasty (PTCA) may in selected cases dramatically improve myocardial blood supply. Seven attempts were made between September and December 1978 at the Gollwitzer-Meier Institute of Cardiology; three dilatations were unsuccessful, two were moderately successful and two highly successful. The problems as to methodology and selection of patients submitted to this procedure are discussed.", "contents": "[Preliminary experience with the catheter dilatation of coronary artery stenoses (author's transl)]. The method of percutaneous transluminal coronary angioplasty (PTCA) may in selected cases dramatically improve myocardial blood supply. Seven attempts were made between September and December 1978 at the Gollwitzer-Meier Institute of Cardiology; three dilatations were unsuccessful, two were moderately successful and two highly successful. The problems as to methodology and selection of patients submitted to this procedure are discussed."}
{"id": "PMID:463188", "title": "[Is hemoperfusion effective in the treatment of digoxin intoxication? (author's transl)].", "content": "Digitalis therapy is frequently accompanied by adverse drug reactions. Severe digitalis intoxications are still a problem. Therapeutical methods, which could be used in the case of a life threatening digoxin intoxication, are known, but not yet generally available. Hemodialysis has only a minor effect on digoxin excretion. This study was planned to test the hypothesis that hemoperfusion across dextran-cocoated charcoal or the resin Amberlite XAD 4 could be more effective in the therapy of digoxin intoxications. The ability of hemoperfusion to eliminate digoxin was tested in a patient who had to undergo treatment beacuse of a severe bromcarbamide intoxication. Additionally we compared the effect of several modifications on this method in 12 dogs, which had received 0.05 mg/kg body weight per day for three days prior to the experiment. Although hemoperfusion across Amberlite XAD 4 may eliminate as much digoxin as normal human kidneys during the few hours of treatment, the amount of digoxin removed after all is only a small percentage of the total body pool. Thus compared to the risks of hemoperfusion as an invasive treatment its effect is small. According to our results, hemoperfusion cannot be recommended as a standard therapy of severe digoxin intoxications.", "contents": "[Is hemoperfusion effective in the treatment of digoxin intoxication? (author's transl)]. Digitalis therapy is frequently accompanied by adverse drug reactions. Severe digitalis intoxications are still a problem. Therapeutical methods, which could be used in the case of a life threatening digoxin intoxication, are known, but not yet generally available. Hemodialysis has only a minor effect on digoxin excretion. This study was planned to test the hypothesis that hemoperfusion across dextran-cocoated charcoal or the resin Amberlite XAD 4 could be more effective in the therapy of digoxin intoxications. The ability of hemoperfusion to eliminate digoxin was tested in a patient who had to undergo treatment beacuse of a severe bromcarbamide intoxication. Additionally we compared the effect of several modifications on this method in 12 dogs, which had received 0.05 mg/kg body weight per day for three days prior to the experiment. Although hemoperfusion across Amberlite XAD 4 may eliminate as much digoxin as normal human kidneys during the few hours of treatment, the amount of digoxin removed after all is only a small percentage of the total body pool. Thus compared to the risks of hemoperfusion as an invasive treatment its effect is small. According to our results, hemoperfusion cannot be recommended as a standard therapy of severe digoxin intoxications."}
{"id": "PMID:463189", "title": "[Electrophysiological mapping during open-heart surgery (author's transl)].", "content": "Intraoperative electrophysiological mapping provides a method to identify the specialized atrioventricular conduction system and to localize the morphological origin of different tachycardiac rhythm disorders at time of surgery. The basic technical aspects and methodical implications are presented with respect to its clinical applications.", "contents": "[Electrophysiological mapping during open-heart surgery (author's transl)]. Intraoperative electrophysiological mapping provides a method to identify the specialized atrioventricular conduction system and to localize the morphological origin of different tachycardiac rhythm disorders at time of surgery. The basic technical aspects and methodical implications are presented with respect to its clinical applications."}
{"id": "PMID:463192", "title": "[Ventricular and myocardial function in aortic regurgitation (author's transl)].", "content": "The influence of chronic volume load on ventricular function and myocardial load and shortening was investigated in 10 patients with chronic aortic regurgitation by means of biplane ventriculography and simultaneous pressure measurements. The regurgitant fraction was 63 +/- 15 per cent. Enddiastolic volume (EDVI 227 +/- 37 ml/m2), systolic (161 +/- 18 mm Hg) and enddiastolic ventricular pressure (30 +/- 12 mm Hg) were elevated, ejection fraction was reduced (54 +/- 7 per cent). Myocardial mass related to EDV was normal (1.2 +/- 0.2 g/ml). Contractility as measured from dp/dt max (1736 +/- 492 mm Hg s-1) and dp/dtmax/PI (22.2 +/- 4.3 s-1) was slightly reduced. Mean velocity of equatorial midwall fiber shortening VMW 0.45 +/- 0.13 cir X s-1) was significantly diminished, equatorial midwall fiber stress during ejection (sigma tej 267 +/- 44.5 X 10(3) dyn X cm-2) was elevated. In spite of a very high enddiastolic stress (sigma ED 96 +/- 36 X 10(3) dyn X cm-2) mean systolic power (sigma tej X VMX 123 +/- 43.2 X 10(3) dyn X cm-2 X s-1) was normal. Compensation of chronic volume load in aortic regurgitation is not compensated by an increased contractility but by ventricular enlargement and a pronouned increase in preload. Myocardial load and shortening in chronic aortic regurgitation are altered in the same way (increased stress and reduced shortening) as in chronic pressure overload.", "contents": "[Ventricular and myocardial function in aortic regurgitation (author's transl)]. The influence of chronic volume load on ventricular function and myocardial load and shortening was investigated in 10 patients with chronic aortic regurgitation by means of biplane ventriculography and simultaneous pressure measurements. The regurgitant fraction was 63 +/- 15 per cent. Enddiastolic volume (EDVI 227 +/- 37 ml/m2), systolic (161 +/- 18 mm Hg) and enddiastolic ventricular pressure (30 +/- 12 mm Hg) were elevated, ejection fraction was reduced (54 +/- 7 per cent). Myocardial mass related to EDV was normal (1.2 +/- 0.2 g/ml). Contractility as measured from dp/dt max (1736 +/- 492 mm Hg s-1) and dp/dtmax/PI (22.2 +/- 4.3 s-1) was slightly reduced. Mean velocity of equatorial midwall fiber shortening VMW 0.45 +/- 0.13 cir X s-1) was significantly diminished, equatorial midwall fiber stress during ejection (sigma tej 267 +/- 44.5 X 10(3) dyn X cm-2) was elevated. In spite of a very high enddiastolic stress (sigma ED 96 +/- 36 X 10(3) dyn X cm-2) mean systolic power (sigma tej X VMX 123 +/- 43.2 X 10(3) dyn X cm-2 X s-1) was normal. Compensation of chronic volume load in aortic regurgitation is not compensated by an increased contractility but by ventricular enlargement and a pronouned increase in preload. Myocardial load and shortening in chronic aortic regurgitation are altered in the same way (increased stress and reduced shortening) as in chronic pressure overload."}
{"id": "PMID:463194", "title": "[Coronary risk factors and extent of angiographically proven coronary artery stenoses (author's transl)].", "content": "In 196 patients with angina pectoris selective coronary angiography was performed, and the extent of angiographically proven coronary artery stenoses was described by means of a coronary score. A significant correlation between the degree of atherosclerotic lesions on the one hand and hypertriglyceridemia, hypercholesterolemia as well as smoking habits on the other hand was detected. No correlation between other risk factors, such as hypertension, diabetes mellitus, hyperuricemia and obesity, and the coronary score was observed.", "contents": "[Coronary risk factors and extent of angiographically proven coronary artery stenoses (author's transl)]. In 196 patients with angina pectoris selective coronary angiography was performed, and the extent of angiographically proven coronary artery stenoses was described by means of a coronary score. A significant correlation between the degree of atherosclerotic lesions on the one hand and hypertriglyceridemia, hypercholesterolemia as well as smoking habits on the other hand was detected. No correlation between other risk factors, such as hypertension, diabetes mellitus, hyperuricemia and obesity, and the coronary score was observed."}
{"id": "PMID:463195", "title": "[Non-invasive effects of cigarette smoking on left ventricular function at rest and with exercise in normal individuals (author's transl)].", "content": "The effect of cigarette smoking on systolic time intervals at rest, with volume overload and with dynamic exercise was studied in nine healthy cigarette smokers. For heart rate, PEP, LVET and blood pressure, reactions to smoking demonstrated considerable interindividual variations with comparable work loads and volume loads. More than half of the subjects developed significant left ventricular dysfunction by these parameters after smoking ten cigarettes. The negative inotropic effect was felt to be caused in these subjects by carbon monoxide. The remaining subjects did not show left ventricular dysfunction with smoking. In these subjects the stimulating effect of nicotine on the nervous system was covering the negative inotropic effect of carboxy hemoglobin.", "contents": "[Non-invasive effects of cigarette smoking on left ventricular function at rest and with exercise in normal individuals (author's transl)]. The effect of cigarette smoking on systolic time intervals at rest, with volume overload and with dynamic exercise was studied in nine healthy cigarette smokers. For heart rate, PEP, LVET and blood pressure, reactions to smoking demonstrated considerable interindividual variations with comparable work loads and volume loads. More than half of the subjects developed significant left ventricular dysfunction by these parameters after smoking ten cigarettes. The negative inotropic effect was felt to be caused in these subjects by carbon monoxide. The remaining subjects did not show left ventricular dysfunction with smoking. In these subjects the stimulating effect of nicotine on the nervous system was covering the negative inotropic effect of carboxy hemoglobin."}
{"id": "PMID:463198", "title": "[Arrhythmias in patients with mitral valve prolapse syndrome. An echo- and electrocardiographic study - (author's transl)].", "content": "The echocardiograms and electrocardiograms of 76 patients with mitral valve prolapse syndrome (MVP) were examined and compared with those of 20 normal subjects. 30 patients had a late systolic, 32 a holosystolic MVP, 14 patients had a mixed motion pattern of the MVP. Eighty percent of them had arrhythmias, which were detected in 44% only by 24-hours ECG, in 32% by 24-hours ECG and exercise ECG and in 4% only by exercise ECG. 16% of the patients had only atrial premature contractions (APC), 22% only ventricular premature contractions (VPC), 39% had both ventricular and atrial premature contractions. Multifocal VPC'S WERE MORE COMMON IN PATIENTS WITH LATE SYSTOLIC MVP, salvos of VPC however were detected in 3 patients with holosystolic and in one patient with late systolic MVP. Only less severe arrhythmias, in general isolated, univorm VPCs were found in 40% of the normal subjects.", "contents": "[Arrhythmias in patients with mitral valve prolapse syndrome. An echo- and electrocardiographic study - (author's transl)]. The echocardiograms and electrocardiograms of 76 patients with mitral valve prolapse syndrome (MVP) were examined and compared with those of 20 normal subjects. 30 patients had a late systolic, 32 a holosystolic MVP, 14 patients had a mixed motion pattern of the MVP. Eighty percent of them had arrhythmias, which were detected in 44% only by 24-hours ECG, in 32% by 24-hours ECG and exercise ECG and in 4% only by exercise ECG. 16% of the patients had only atrial premature contractions (APC), 22% only ventricular premature contractions (VPC), 39% had both ventricular and atrial premature contractions. Multifocal VPC'S WERE MORE COMMON IN PATIENTS WITH LATE SYSTOLIC MVP, salvos of VPC however were detected in 3 patients with holosystolic and in one patient with late systolic MVP. Only less severe arrhythmias, in general isolated, univorm VPCs were found in 40% of the normal subjects."}
{"id": "PMID:463201", "title": "[\"Words\" in orthopaedics (author's transl)].", "content": "The interpretation of the words in the detailed and prolix terminology by Andry conveys some of the essential contents of modern orthopaedics, but completely ignores another branch of orthopaedics in adults, particularly its most important source, namely, traumatology of the locomotor organs.", "contents": "[\"Words\" in orthopaedics (author's transl)]. The interpretation of the words in the detailed and prolix terminology by Andry conveys some of the essential contents of modern orthopaedics, but completely ignores another branch of orthopaedics in adults, particularly its most important source, namely, traumatology of the locomotor organs."}
{"id": "PMID:463202", "title": "[Problems and risks of functional treatment of hip joint dysplasia in early infancy (author's transl)].", "content": "Initial difficulties arise already in diagnostic stage. We cannot say whether a hip will spontaneously develop into a normal one. Hence, we must treat all children showing signs of dysplasia, and we must be willing to accept being accused of polypragmasis. There is also a certain, albeit slight, risk of disturbing the development of the femoral head. The must be strictly avoided by exercising greater possible care with regard to surgical technique and follow-up control. It is precisely the apparent simplicity of the method which presents a major difficulty. The article goes into the details of the method. Prevention in early infancy is the only possibility of checking the disease in all its stages.", "contents": "[Problems and risks of functional treatment of hip joint dysplasia in early infancy (author's transl)]. Initial difficulties arise already in diagnostic stage. We cannot say whether a hip will spontaneously develop into a normal one. Hence, we must treat all children showing signs of dysplasia, and we must be willing to accept being accused of polypragmasis. There is also a certain, albeit slight, risk of disturbing the development of the femoral head. The must be strictly avoided by exercising greater possible care with regard to surgical technique and follow-up control. It is precisely the apparent simplicity of the method which presents a major difficulty. The article goes into the details of the method. Prevention in early infancy is the only possibility of checking the disease in all its stages."}
{"id": "PMID:463203", "title": "[Planning of a complex intertrochanteric osteotomy (author's transl)].", "content": "The article describes in detail the preoperative planning on a complex intertrochenteric osteotomy with eight corrections. Attention is drawn to two main complication risks and how to avoid them, namely, necrosis of the head of the femur and tilting of the proximal fragment when inserting the blade of the rectangular plate.", "contents": "[Planning of a complex intertrochanteric osteotomy (author's transl)]. The article describes in detail the preoperative planning on a complex intertrochenteric osteotomy with eight corrections. Attention is drawn to two main complication risks and how to avoid them, namely, necrosis of the head of the femur and tilting of the proximal fragment when inserting the blade of the rectangular plate."}
{"id": "PMID:463204", "title": "[Comments on the double-cup hip endoprosthesis according to Freeman (author's transl)].", "content": "Histological examinations (including tetracycline labelling) of preparations of the neck of the femur which had to be removed 1 1/2, 3 and 14 1/2 months subsequent to double-cup arthroplasty according to Freeman, prove that the bone tissue can remain vital under the solidly cemented metal cup. A thick layer of connective tissue with bundles of collagenous fibres running parallel to the surface, is temporarily formed between the cement layer and the prepared spongiosa of the neck of the femur. It is possible that this layer of connective tissue is replaced within about a years' time by a thin, lamellar cortex layer, which is then covered only by a thin layer of connective tissue which is rich in fibres. Below this layer, the spongiosa spaces are definitely widened and filled with fibrotic fat marrow; vascularisation decreases, whereas osteoporosis increases.", "contents": "[Comments on the double-cup hip endoprosthesis according to Freeman (author's transl)]. Histological examinations (including tetracycline labelling) of preparations of the neck of the femur which had to be removed 1 1/2, 3 and 14 1/2 months subsequent to double-cup arthroplasty according to Freeman, prove that the bone tissue can remain vital under the solidly cemented metal cup. A thick layer of connective tissue with bundles of collagenous fibres running parallel to the surface, is temporarily formed between the cement layer and the prepared spongiosa of the neck of the femur. It is possible that this layer of connective tissue is replaced within about a years' time by a thin, lamellar cortex layer, which is then covered only by a thin layer of connective tissue which is rich in fibres. Below this layer, the spongiosa spaces are definitely widened and filled with fibrotic fat marrow; vascularisation decreases, whereas osteoporosis increases."}
{"id": "PMID:463205", "title": "[Discussion on the substitution of anterior crucial ligament in knee capsule instabilities (author's transl)].", "content": "The conventional methods of rehabilitation plasty of the insufficient anterior crucial ligament will usually display elasticity in course of time. Accurate reconstructions of all capsular ligament lesions and the reinforcement by threads of the requisite connective tissue transplants, show good stability and a good overall result four years after the operation in a relatively small number of patients.", "contents": "[Discussion on the substitution of anterior crucial ligament in knee capsule instabilities (author's transl)]. The conventional methods of rehabilitation plasty of the insufficient anterior crucial ligament will usually display elasticity in course of time. Accurate reconstructions of all capsular ligament lesions and the reinforcement by threads of the requisite connective tissue transplants, show good stability and a good overall result four years after the operation in a relatively small number of patients."}
{"id": "PMID:463206", "title": "[Narrowness of the recessus lateralis in the lumbar region of the spine as cause of the nerve-root compression in narrowing of the intervertebral discs (author's transl)].", "content": "The intense radicular pain of sciatica may result from nerve root entrapment in a narrowed lateral vertebral recess without discal herniation. In such cases relief of pain is achieved by unroofing the lateral recess and excising the overhanging portion of the superior articular facet, without removing the disc. The narrowing of the lateral recess is congenital, but the compression of the root occurs first in adult life. The thinning of the disc causes backward displacement of the vertebral body above and forward displacement of the cranial articular facet of the vertebral body below the thinned disc. In a large lateral recess such subluxation does not cause any entrapment of the root. If the lateral recess is congenitally narrowed the root is tightly wedged laterally and sometimes can be decompressed only by a complete arthrotomy. The same entrapment can occur as a consequence of removing of a disc (pseudorecurrence).", "contents": "[Narrowness of the recessus lateralis in the lumbar region of the spine as cause of the nerve-root compression in narrowing of the intervertebral discs (author's transl)]. The intense radicular pain of sciatica may result from nerve root entrapment in a narrowed lateral vertebral recess without discal herniation. In such cases relief of pain is achieved by unroofing the lateral recess and excising the overhanging portion of the superior articular facet, without removing the disc. The narrowing of the lateral recess is congenital, but the compression of the root occurs first in adult life. The thinning of the disc causes backward displacement of the vertebral body above and forward displacement of the cranial articular facet of the vertebral body below the thinned disc. In a large lateral recess such subluxation does not cause any entrapment of the root. If the lateral recess is congenitally narrowed the root is tightly wedged laterally and sometimes can be decompressed only by a complete arthrotomy. The same entrapment can occur as a consequence of removing of a disc (pseudorecurrence)."}
{"id": "PMID:463207", "title": "[Anterior interbody fusion with neurological symptoms of a spondylolisthesis (author's transl)].", "content": "28 patients with spondylolisthesis who underwent anterior interbody fusion at the Orthopaedic Department of the University of Basle between 1968 and 1976, have been follow-up. In 90% satisfactory or good clinical results were achieved. In 82% the neurological symptoms - without laminectomy - were markedly improved or eliminated. According to the authors anterior interbody fusion (with reduction and enlargement of the intervetebral space) is indicated even when neurological symptoms are present.", "contents": "[Anterior interbody fusion with neurological symptoms of a spondylolisthesis (author's transl)]. 28 patients with spondylolisthesis who underwent anterior interbody fusion at the Orthopaedic Department of the University of Basle between 1968 and 1976, have been follow-up. In 90% satisfactory or good clinical results were achieved. In 82% the neurological symptoms - without laminectomy - were markedly improved or eliminated. According to the authors anterior interbody fusion (with reduction and enlargement of the intervetebral space) is indicated even when neurological symptoms are present."}
{"id": "PMID:463208", "title": "[The operative therapy of the acquired pes plano-valgus and pes planus of the child (author's transl)].", "content": "The author shows the importance of the development of the leg axis for the diagnosis and the indication for the treatment of the acquired infantile pes plano-valgus and pes planus. Only when this development is finished an operative therapy can be indicated. The experience gained on a very treat number ofpatients shows that it is evident that the best method is the transposition of the tendons M. fib. III or IV. The author also discusses the reasons for indication and the limitation of this operation. (The author prefers the modified techniques of K. Niederecker (1959) based on the idea of E. M\u00fcller (1903).", "contents": "[The operative therapy of the acquired pes plano-valgus and pes planus of the child (author's transl)]. The author shows the importance of the development of the leg axis for the diagnosis and the indication for the treatment of the acquired infantile pes plano-valgus and pes planus. Only when this development is finished an operative therapy can be indicated. The experience gained on a very treat number ofpatients shows that it is evident that the best method is the transposition of the tendons M. fib. III or IV. The author also discusses the reasons for indication and the limitation of this operation. (The author prefers the modified techniques of K. Niederecker (1959) based on the idea of E. M\u00fcller (1903)."}
{"id": "PMID:463209", "title": "[Indication and assessment of the surgical treatment of fractures of the os calcis (author's transl)].", "content": "The instable fractures of os calcis are usually treated by conservative methods with early functional mobilization. In a few cases we think that in the Tongue Type and especially in the Joint Depression Type of Essex-Lopresti a surgical treatment may be useful to reestablish the congruence and the gometry of the joint surfaces. Some typical cases are described. Even if this active treatment of fractures of the os calcis may be generally regarded as hazardous, we think that especially in young selected individuals an operative anatomical reduction and stable fixation may be appropriate.", "contents": "[Indication and assessment of the surgical treatment of fractures of the os calcis (author's transl)]. The instable fractures of os calcis are usually treated by conservative methods with early functional mobilization. In a few cases we think that in the Tongue Type and especially in the Joint Depression Type of Essex-Lopresti a surgical treatment may be useful to reestablish the congruence and the gometry of the joint surfaces. Some typical cases are described. Even if this active treatment of fractures of the os calcis may be generally regarded as hazardous, we think that especially in young selected individuals an operative anatomical reduction and stable fixation may be appropriate."}
{"id": "PMID:463210", "title": "[Dwyer's calcaneal osteotomy for varus deformity of the foot. (A follow-up study including gait analysis) (author's transl)].", "content": "A follow-up study on 20 patients one to 9 years after lateral wedge resection of the calcaneus according to Dwyer is reported. In 18 feet with neurogenic varus deformity of the hind foot, the angle between the axis of the lower leg and the heel was altered from 11.4 degrees +/- 1.4 degrees SD varus to a normal value of 5.9 degrees +/- 0.9 degrees SD valgus. The position of the calcaneal tuberosity was changed from 6.7 degrees +/- 1.1 degree SD varus to 0.1 degree +/- SD varus in 7 congenital club feet. The osteotomy reduced accompanying deformities of pes cavus and pes adductus in the operated children.", "contents": "[Dwyer's calcaneal osteotomy for varus deformity of the foot. (A follow-up study including gait analysis) (author's transl)]. A follow-up study on 20 patients one to 9 years after lateral wedge resection of the calcaneus according to Dwyer is reported. In 18 feet with neurogenic varus deformity of the hind foot, the angle between the axis of the lower leg and the heel was altered from 11.4 degrees +/- 1.4 degrees SD varus to a normal value of 5.9 degrees +/- 0.9 degrees SD valgus. The position of the calcaneal tuberosity was changed from 6.7 degrees +/- 1.1 degree SD varus to 0.1 degree +/- SD varus in 7 congenital club feet. The osteotomy reduced accompanying deformities of pes cavus and pes adductus in the operated children."}
{"id": "PMID:463211", "title": "[Tenosynovectomy in rheumatoid arthritis (author's transl)].", "content": "Tenosynovitis is much more frequent in RA than supposed. A comparative statistic of joint- and tenosynovectomy of the Clinic Wilhelm Schulthess is presented. It also proves the importance of flexor tendon involvement. From the patient's point of view the result is good in more than 95% 6 years in average after synovectomy. Recurrent synovitis is very rare in the extensor tendon. It may occur in the extensor carpi ulnaris. In the flexor compartement the symptoms disappear also in most instance. The reasons are discussed and figures given of our statistic.", "contents": "[Tenosynovectomy in rheumatoid arthritis (author's transl)]. Tenosynovitis is much more frequent in RA than supposed. A comparative statistic of joint- and tenosynovectomy of the Clinic Wilhelm Schulthess is presented. It also proves the importance of flexor tendon involvement. From the patient's point of view the result is good in more than 95% 6 years in average after synovectomy. Recurrent synovitis is very rare in the extensor tendon. It may occur in the extensor carpi ulnaris. In the flexor compartement the symptoms disappear also in most instance. The reasons are discussed and figures given of our statistic."}
{"id": "PMID:463212", "title": "[Temporary plugging of cystic bone tumors by bone cement (author's transl)].", "content": "Methylmethacrylate bone cement was used to refill bony defects following excisional biopsy of supposed benign or semimalign bone tumors. This procedure offers several advantages: the anatomical situation at the site of the lesion will not be altered, that means the functions of the joint and the continuity and stability of the bone will be preserved; the histological examination of the tissue is possible without a hurry; the follow up of the lesion is easily possible by X-ray-examination; further therapeutic procedures can follow without restriction, for example if the histology discovered an unsuspected malignant tumor or if the follow-up revealed a recurrency. In addition a favorable effect is the necrosis of tumor cells, eventually left behind in the bone, by the action of zytotoxic monomer and heat, originated during the polymerisation of the methylmethacrylate. In benign or semimalignant bone tumors the cement has to be removed after an adequate observation period; at this occasion the cavity again is curetted and then filled with autologous bone grafts. Since 1972 we treated 13 bone lesions by this method of \"temporary bone cement plugging\". The lesions were 5 giant cell tumors, 2 aneurysmal bone cysts, 2 simple bone cysts, 1 osteosarcoma, 1 malignant lymphoma, and 2 metastases of hypernephroid carcinoma. In the case of osteosarcoma an amputation was performed just after the diagnosis was made. In the other cases no local recurrances up to now were seen.", "contents": "[Temporary plugging of cystic bone tumors by bone cement (author's transl)]. Methylmethacrylate bone cement was used to refill bony defects following excisional biopsy of supposed benign or semimalign bone tumors. This procedure offers several advantages: the anatomical situation at the site of the lesion will not be altered, that means the functions of the joint and the continuity and stability of the bone will be preserved; the histological examination of the tissue is possible without a hurry; the follow up of the lesion is easily possible by X-ray-examination; further therapeutic procedures can follow without restriction, for example if the histology discovered an unsuspected malignant tumor or if the follow-up revealed a recurrency. In addition a favorable effect is the necrosis of tumor cells, eventually left behind in the bone, by the action of zytotoxic monomer and heat, originated during the polymerisation of the methylmethacrylate. In benign or semimalignant bone tumors the cement has to be removed after an adequate observation period; at this occasion the cavity again is curetted and then filled with autologous bone grafts. Since 1972 we treated 13 bone lesions by this method of \"temporary bone cement plugging\". The lesions were 5 giant cell tumors, 2 aneurysmal bone cysts, 2 simple bone cysts, 1 osteosarcoma, 1 malignant lymphoma, and 2 metastases of hypernephroid carcinoma. In the case of osteosarcoma an amputation was performed just after the diagnosis was made. In the other cases no local recurrances up to now were seen."}
{"id": "PMID:463213", "title": "[Progresses in the orthopedic-reconstructive surgery by microsurgery (author's transl)].", "content": "After a short description of the microsurgical technique for operations of periphere nerves and small vessels, the possibilities for reconstructive operations in orthopedic surgery are demonstrated. The actual experiences allow a critical but also cautious prospective statement.", "contents": "[Progresses in the orthopedic-reconstructive surgery by microsurgery (author's transl)]. After a short description of the microsurgical technique for operations of periphere nerves and small vessels, the possibilities for reconstructive operations in orthopedic surgery are demonstrated. The actual experiences allow a critical but also cautious prospective statement."}
{"id": "PMID:463214", "title": "[Differential diagnosis of osteoporosis (author's transl)].", "content": "This is a review of the diagnostic procedures used in patients with osteoporosis. The differential diagnosis of various forms with recognized etiology is used as a background for the study of postmenopausal osteoporosis with largely unknown pathogenesis.", "contents": "[Differential diagnosis of osteoporosis (author's transl)]. This is a review of the diagnostic procedures used in patients with osteoporosis. The differential diagnosis of various forms with recognized etiology is used as a background for the study of postmenopausal osteoporosis with largely unknown pathogenesis."}
{"id": "PMID:463215", "title": "[Standing and walking with walking aids - an electromyokinesigraphic examination (author's transl)].", "content": "Study of the variations of the automatic electrical activity of the shoulder, trunk and lower extremities muscles with different types of walking aids (Cane, elbow-crutches, shoulder-crutches, tricycle). Electromyocinesigraphic recordings have been made in 10 normal men and women age 24 to 35. The variations of their posture and walking pattern according to the weightbearing with different types of aids have been studied and are discussed.", "contents": "[Standing and walking with walking aids - an electromyokinesigraphic examination (author's transl)]. Study of the variations of the automatic electrical activity of the shoulder, trunk and lower extremities muscles with different types of walking aids (Cane, elbow-crutches, shoulder-crutches, tricycle). Electromyocinesigraphic recordings have been made in 10 normal men and women age 24 to 35. The variations of their posture and walking pattern according to the weightbearing with different types of aids have been studied and are discussed."}
{"id": "PMID:463216", "title": "[The Erlangen hip brace, a principle of rehabilitative treatment (author's transl)].", "content": "Painful, loosened total hip prostheses and instability following their removal require up to now occasionally support by means of a brace. The paper deals with design, way of action and indication of a new, light-weight, dynamic brace to controle the motion of hip joint. It is evident that this brace can be used not only to manage the loosening of a total hip prosthesis or conditions following the resection of the head of femur, but also in osteoarthrosis of hip joint.", "contents": "[The Erlangen hip brace, a principle of rehabilitative treatment (author's transl)]. Painful, loosened total hip prostheses and instability following their removal require up to now occasionally support by means of a brace. The paper deals with design, way of action and indication of a new, light-weight, dynamic brace to controle the motion of hip joint. It is evident that this brace can be used not only to manage the loosening of a total hip prosthesis or conditions following the resection of the head of femur, but also in osteoarthrosis of hip joint."}
{"id": "PMID:463217", "title": "[The use of carbon as manufacturing material of endoprotheses (author's transl)].", "content": "According to the present stage of technology, arthroplasties have a change of only short-term to medium-term success quotas despite good initial successes. Permanent healing is restricted because of possible loosening of the prostheses. Even clinically silent loosenings will ultimately lead to failures, either by progressive degeneration of the bones at the supporting points of the prosthesis or through breakage of the stem of the prostheses. Attempts to overcome such difficulties by changing the shape of the prostheses, remain unsuccessful. The material employed in the construction of the prosthesis is certainly of some importance in this connection. Carbon has been known for a long time as a well-tolerated material in implant surgery. Carbon is indeed distinguished by high biocompatibility. The development of new carbons based on graphite which considerably improve its physical properties, has enabled the manufacture of endoprostheses made from carbon. Studies have proved that this substance is nontoxic. There are also no immunological reactions. Abrasion is low, and the abraded particles do not produce any severe inflammatory reactions. Hence, prostheses made from carbon can be employed as combination constructions, using suitable types of carbon in accordance with the required functions.", "contents": "[The use of carbon as manufacturing material of endoprotheses (author's transl)]. According to the present stage of technology, arthroplasties have a change of only short-term to medium-term success quotas despite good initial successes. Permanent healing is restricted because of possible loosening of the prostheses. Even clinically silent loosenings will ultimately lead to failures, either by progressive degeneration of the bones at the supporting points of the prosthesis or through breakage of the stem of the prostheses. Attempts to overcome such difficulties by changing the shape of the prostheses, remain unsuccessful. The material employed in the construction of the prosthesis is certainly of some importance in this connection. Carbon has been known for a long time as a well-tolerated material in implant surgery. Carbon is indeed distinguished by high biocompatibility. The development of new carbons based on graphite which considerably improve its physical properties, has enabled the manufacture of endoprostheses made from carbon. Studies have proved that this substance is nontoxic. There are also no immunological reactions. Abrasion is low, and the abraded particles do not produce any severe inflammatory reactions. Hence, prostheses made from carbon can be employed as combination constructions, using suitable types of carbon in accordance with the required functions."}
{"id": "PMID:463218", "title": "[A contribution to the differential indication of endoprosthetic knee-joint substitution (author's transl)].", "content": "The article describes a few guiding points for indicating the need for a knee-joint endoprosthesis, basing on experience with 253 cases, followed up for 2--48 months. Following the application of prostheses according to Walldius-Debeyre and Guepar, several complications were seen which we tried to reduce by introducing four prostheses of different sizes, since the choice of the implantates could be adapted to the prevailing anatomical conditions. A hinged-joint prosthesis of the St. George type is indicated in cases of most severe axial deviation, severe flexion contraction, extensive loss of bone substance, and instability of the ligaments. Sheeban's prosthesis is indicated in severe damage involving both compartments, limited frontal axial deviation, and flexion contraction of not more than 25 degrees with intact lateral ligaments. In younger patients with rheumatoid arthritis we implant the Geomedic prosthesis. If only one knee-joint compartment is involved, and after the possibility of readjustment osteotomy has been excluded, we consider a sliding prosthesis to be the prosthesis of choice.", "contents": "[A contribution to the differential indication of endoprosthetic knee-joint substitution (author's transl)]. The article describes a few guiding points for indicating the need for a knee-joint endoprosthesis, basing on experience with 253 cases, followed up for 2--48 months. Following the application of prostheses according to Walldius-Debeyre and Guepar, several complications were seen which we tried to reduce by introducing four prostheses of different sizes, since the choice of the implantates could be adapted to the prevailing anatomical conditions. A hinged-joint prosthesis of the St. George type is indicated in cases of most severe axial deviation, severe flexion contraction, extensive loss of bone substance, and instability of the ligaments. Sheeban's prosthesis is indicated in severe damage involving both compartments, limited frontal axial deviation, and flexion contraction of not more than 25 degrees with intact lateral ligaments. In younger patients with rheumatoid arthritis we implant the Geomedic prosthesis. If only one knee-joint compartment is involved, and after the possibility of readjustment osteotomy has been excluded, we consider a sliding prosthesis to be the prosthesis of choice."}
{"id": "PMID:463219", "title": "[Histological and morphometric investigations of knee joint synovial membrane concerning diabetic arthropathy (author's transl)].", "content": "Joint capsular tissue from both knee joints have been investigated light microscopically and morphometrically in 40 cases with maturity onset of diabetes. The tissue specimen were taken from autopsy cases (27 female, 13 male). The following alterations of synovial tissue can be regarded as typically in diabetes mellitus: 1. An increased formation of synovial villi without proliferation of synoviocytes and without inflammatory reaction. 2. Increased hyalinosis and fibrosis of the synovial villi as well as fibrosis of the flat synovial membrane. The alterations were far developed in more than 50% of all cases with diabetes.", "contents": "[Histological and morphometric investigations of knee joint synovial membrane concerning diabetic arthropathy (author's transl)]. Joint capsular tissue from both knee joints have been investigated light microscopically and morphometrically in 40 cases with maturity onset of diabetes. The tissue specimen were taken from autopsy cases (27 female, 13 male). The following alterations of synovial tissue can be regarded as typically in diabetes mellitus: 1. An increased formation of synovial villi without proliferation of synoviocytes and without inflammatory reaction. 2. Increased hyalinosis and fibrosis of the synovial villi as well as fibrosis of the flat synovial membrane. The alterations were far developed in more than 50% of all cases with diabetes."}
{"id": "PMID:463221", "title": "[Observation of the so-called \"anti-Chiari effect\" subsequent to osteotomy of the pelvis in children (author's transl)].", "content": "Osteotomy of the pelvis according to Chiari is performed in the immediate vicinity of the acetabulum and the craniolateral corner of the acetabulum. In children, it will almost always involve damaging the important growth zones of the acetabulum. Depending on the site and extent of the osteotomy performed, the pathological growth kinetics will lead to varying defects. This pathology of the kinetics of growth is known as \"Anti-Chiari effect\".", "contents": "[Observation of the so-called \"anti-Chiari effect\" subsequent to osteotomy of the pelvis in children (author's transl)]. Osteotomy of the pelvis according to Chiari is performed in the immediate vicinity of the acetabulum and the craniolateral corner of the acetabulum. In children, it will almost always involve damaging the important growth zones of the acetabulum. Depending on the site and extent of the osteotomy performed, the pathological growth kinetics will lead to varying defects. This pathology of the kinetics of growth is known as \"Anti-Chiari effect\"."}
{"id": "PMID:463222", "title": "[Osteochondrosis dissecans of the elbow joint and the therapy depending on stage of development (author's transl)].", "content": "Of 46 cases of Osteochondrosis dissecans (OD) of the elbow joint between 1949 and 1976 in 35 patients we could control 31 patients with totally 42 diseases. According to clinical symptoms and roentgenological signs we classified the OD in an early, medium and late stage of development. The success of the therapy was discussed depending on the method of therapy, on the stage of the disease and on the age of the patients.", "contents": "[Osteochondrosis dissecans of the elbow joint and the therapy depending on stage of development (author's transl)]. Of 46 cases of Osteochondrosis dissecans (OD) of the elbow joint between 1949 and 1976 in 35 patients we could control 31 patients with totally 42 diseases. According to clinical symptoms and roentgenological signs we classified the OD in an early, medium and late stage of development. The success of the therapy was discussed depending on the method of therapy, on the stage of the disease and on the age of the patients."}
{"id": "PMID:463223", "title": "[Ultrastructural investigations of the palmarfascia in M. Dupuytren (author's transl)].", "content": "The palmarfascia of 30 patients with Dupuytrens disease was investigated light- and electron microscopically. As typical changes there exist an increase in cells which form noduli. Many cells contain contractile filaments in their cytoplasma are therefore described as myofibroblasts. Beside these cells active fibroblasts could be observed. Close to their cell membranes there occur filaments with a diameter of 100--300 A. In a greater distance fibrils and fibers with a diameter of 400--600 A were observed. The regular order of the connective tissue is disturbed, one often can observe \"vortex\" like structures. The lumina of many capillaries are collapsed. The pericytes are increased. The nerve fibers are morphologically unchanged - they are often closely surrounded by collagen fibers. Respecting the morphological findings the pathogenesis of Dupuytrens disease is discussed.", "contents": "[Ultrastructural investigations of the palmarfascia in M. Dupuytren (author's transl)]. The palmarfascia of 30 patients with Dupuytrens disease was investigated light- and electron microscopically. As typical changes there exist an increase in cells which form noduli. Many cells contain contractile filaments in their cytoplasma are therefore described as myofibroblasts. Beside these cells active fibroblasts could be observed. Close to their cell membranes there occur filaments with a diameter of 100--300 A. In a greater distance fibrils and fibers with a diameter of 400--600 A were observed. The regular order of the connective tissue is disturbed, one often can observe \"vortex\" like structures. The lumina of many capillaries are collapsed. The pericytes are increased. The nerve fibers are morphologically unchanged - they are often closely surrounded by collagen fibers. Respecting the morphological findings the pathogenesis of Dupuytrens disease is discussed."}
{"id": "PMID:463225", "title": "[The lengths' difference of legs in view of the general physician (author's transl)].", "content": "The present paper treats the problem of the lengths' difference of legs in view of the general physician. After a five years' research a survey has been established and a classification in four groups has been made. Moreover the question of diagnosis and therapy has been treated. The requirement has been raised to diagnose as early as possible and to apply the corresponding therapy even in case of a lengths' difference below 20 mm in order to avoid any inevitable secondary consequences. Only by acting this way arising complaints, long working incapabilities and last but not least considerable expenses can be prevented.", "contents": "[The lengths' difference of legs in view of the general physician (author's transl)]. The present paper treats the problem of the lengths' difference of legs in view of the general physician. After a five years' research a survey has been established and a classification in four groups has been made. Moreover the question of diagnosis and therapy has been treated. The requirement has been raised to diagnose as early as possible and to apply the corresponding therapy even in case of a lengths' difference below 20 mm in order to avoid any inevitable secondary consequences. Only by acting this way arising complaints, long working incapabilities and last but not least considerable expenses can be prevented."}
{"id": "PMID:463226", "title": "[Investigations on hips and extremities by ultrasonics (author's transl)].", "content": "Using a Picker Echoview System 80 L, an Ultrasound Compound scanning instrument from Picker Roentgen GmbH, Espelkamp, Germany, some investigations on bones and joints were performed. In comparison with needle probe diameter measuring of muscles it could be shown that ultrasound tomography gives identical results without pain. Figuring of hip and knee joints outlines was possible. Alterations of muscles and tendon tissue were to be seen. Special meridians on the hip joint, along with the ultrasound probe had to be moved, were defined by anatomical marking points. So it became possible to reproduce some angles which usually had to be measured at X-ray pictures.", "contents": "[Investigations on hips and extremities by ultrasonics (author's transl)]. Using a Picker Echoview System 80 L, an Ultrasound Compound scanning instrument from Picker Roentgen GmbH, Espelkamp, Germany, some investigations on bones and joints were performed. In comparison with needle probe diameter measuring of muscles it could be shown that ultrasound tomography gives identical results without pain. Figuring of hip and knee joints outlines was possible. Alterations of muscles and tendon tissue were to be seen. Special meridians on the hip joint, along with the ultrasound probe had to be moved, were defined by anatomical marking points. So it became possible to reproduce some angles which usually had to be measured at X-ray pictures."}
{"id": "PMID:463227", "title": "[Late results of the operated patients with femoral neck fractures. results of the a-o technique and the endoprosthesis from 3 to 5 years after operation (author's transl)].", "content": "The late results of 133 operatively treated patients with femoral neck and trochanteric fractures were evaluated. The series consisted of 86 women and 47 men, 72 femoral neck fractures and 61 trochanteric fractures. 99 patients were treated by A-O osteosynthesis and 34 by endoprosthesis. The average age of the group with osteosynthesis was 71 and of the group with endoprosthesis 76 years. From 3 to 5 years after the operation the nailing results of the patients with femoral neck fractures were poor in 7.1 and with trochanteric fractures in 15.4 per cent. During the same observation period the results of the patients treated with endoprosthesis were poor in 14.3 per cent. There were no statistically significant difference between the A-O group and the endoprosthesis group with femoral neck fractures. Between the mortality of the nailed patients with femoral neck fractures and the endoprosthesis group there was no statistically significant difference.", "contents": "[Late results of the operated patients with femoral neck fractures. results of the a-o technique and the endoprosthesis from 3 to 5 years after operation (author's transl)]. The late results of 133 operatively treated patients with femoral neck and trochanteric fractures were evaluated. The series consisted of 86 women and 47 men, 72 femoral neck fractures and 61 trochanteric fractures. 99 patients were treated by A-O osteosynthesis and 34 by endoprosthesis. The average age of the group with osteosynthesis was 71 and of the group with endoprosthesis 76 years. From 3 to 5 years after the operation the nailing results of the patients with femoral neck fractures were poor in 7.1 and with trochanteric fractures in 15.4 per cent. During the same observation period the results of the patients treated with endoprosthesis were poor in 14.3 per cent. There were no statistically significant difference between the A-O group and the endoprosthesis group with femoral neck fractures. Between the mortality of the nailed patients with femoral neck fractures and the endoprosthesis group there was no statistically significant difference."}
{"id": "PMID:463228", "title": "[Intraosseous osteotomy in arthrosis of the knee joint (author's transl)].", "content": "The article describes a surgical method in which removal of the spongiosa is effected in the region of the upper tibia and the lower femur after fenestration of the cortex. Results are reported on the surgical treatment of 24 knee joints with painful arthrosis without any deformation worth mentioning. Worthwhile pain relief was achieved in 22 cases. In these cases, no osteotomy with correction was indicated.", "contents": "[Intraosseous osteotomy in arthrosis of the knee joint (author's transl)]. The article describes a surgical method in which removal of the spongiosa is effected in the region of the upper tibia and the lower femur after fenestration of the cortex. Results are reported on the surgical treatment of 24 knee joints with painful arthrosis without any deformation worth mentioning. Worthwhile pain relief was achieved in 22 cases. In these cases, no osteotomy with correction was indicated."}
{"id": "PMID:463229", "title": "[A contribution to pigmented villonodular synovitis of the shoulder joint (author's transl)].", "content": "Pigmented villonodular synovitis of the shoulder joint, particularly with osseous invasion, is a rare disease. Typical clinical, roentgenological and histological criteria are pointed out on the basis of the case report of one of the authors' own patients. Aetiology and pathogenesis of the changes are discussed. This disease must be included in differential diagnostic deliberations in the shoulder joint region as well, where it may be superficially confused with malignant tumours of the joint.", "contents": "[A contribution to pigmented villonodular synovitis of the shoulder joint (author's transl)]. Pigmented villonodular synovitis of the shoulder joint, particularly with osseous invasion, is a rare disease. Typical clinical, roentgenological and histological criteria are pointed out on the basis of the case report of one of the authors' own patients. Aetiology and pathogenesis of the changes are discussed. This disease must be included in differential diagnostic deliberations in the shoulder joint region as well, where it may be superficially confused with malignant tumours of the joint."}
{"id": "PMID:463230", "title": "[Functional adaptation in transnaviculolunate resectionarthroplasty (Steinh\u00e4user operation) (author's transl)].", "content": "The functional status of the new wrist joint after transnaviculo-lunate resectionarthroplasty is examined with a 12 year follow-up. The biomechanical and functional changes induced by total os lunatum and partial os navicularis resection are demonstrated. The resulting degenerative changes remain within tolerably acceptable limits after this long period. Clinically, we find a stable, almost completely pain free joint with good motion and good stress tolerance.", "contents": "[Functional adaptation in transnaviculolunate resectionarthroplasty (Steinh\u00e4user operation) (author's transl)]. The functional status of the new wrist joint after transnaviculo-lunate resectionarthroplasty is examined with a 12 year follow-up. The biomechanical and functional changes induced by total os lunatum and partial os navicularis resection are demonstrated. The resulting degenerative changes remain within tolerably acceptable limits after this long period. Clinically, we find a stable, almost completely pain free joint with good motion and good stress tolerance."}
{"id": "PMID:463231", "title": "[Recessus and ganglia (author's transl)].", "content": "Preparated, communicating Recessus - Bursa suprapatellaris, Recessus popliteus and Bursa musculi gastrocnemii medialis - will be compared with ganglia. The surfaces document conformity. The REM-controls show a further proof of the gangliagenesis from recessus.", "contents": "[Recessus and ganglia (author's transl)]. Preparated, communicating Recessus - Bursa suprapatellaris, Recessus popliteus and Bursa musculi gastrocnemii medialis - will be compared with ganglia. The surfaces document conformity. The REM-controls show a further proof of the gangliagenesis from recessus."}
{"id": "PMID:463232", "title": "[Angioma like dysplasia of synovial membrane with phlebectasia causing palindromic joint swelling in childhood (author's transl)].", "content": "In a five year old boy who had intermittent knee joint swelling, angioma like venous dysplasia of synovial membrane was diagnosed by arthroscopic examination. The arthroscopic diagnosis was the indication for arthrotomy. The suprapatella pouch of the knee joint contained a blue grape like tumor. Angiomalike venous dysplasia of the synovialmembrane can histologically be separated from other angiomas of the inner joint capsule and angiomalike alterations in other joint diseases. Arthroscopy should be performed if within half a year monarticular intermittent joint swelling in childhood can not be diagnosed.", "contents": "[Angioma like dysplasia of synovial membrane with phlebectasia causing palindromic joint swelling in childhood (author's transl)]. In a five year old boy who had intermittent knee joint swelling, angioma like venous dysplasia of synovial membrane was diagnosed by arthroscopic examination. The arthroscopic diagnosis was the indication for arthrotomy. The suprapatella pouch of the knee joint contained a blue grape like tumor. Angiomalike venous dysplasia of the synovialmembrane can histologically be separated from other angiomas of the inner joint capsule and angiomalike alterations in other joint diseases. Arthroscopy should be performed if within half a year monarticular intermittent joint swelling in childhood can not be diagnosed."}
{"id": "PMID:463233", "title": "[Experimental investigations on the pathogenesis of achillis tendon rupture (author's transl)].", "content": "The histological changes following ischemic alteration of achillis tendon in rabbits are similar to those found in ruptured human achillis tendons. The healing of the experimentally damaged tendons is equal to that found in humans. Those results suggest that at least in a part of cases ischemic causes achillis tendon rupture.", "contents": "[Experimental investigations on the pathogenesis of achillis tendon rupture (author's transl)]. The histological changes following ischemic alteration of achillis tendon in rabbits are similar to those found in ruptured human achillis tendons. The healing of the experimentally damaged tendons is equal to that found in humans. Those results suggest that at least in a part of cases ischemic causes achillis tendon rupture."}
{"id": "PMID:463255", "title": "Renal osteodystrophy in children with end-stage kidney failure.", "content": "The discovery that the kidney is the site of production of calcitriol clarifies our understanding of calcium metabolic disorders in chronic renal failure and carries with it clinical implications in the treatment of renal rickets. The author presents data on the action of calcium and reviews the therapeutic effects on uremic osteodystrophy in children of calcitriol and its analogue.", "contents": "Renal osteodystrophy in children with end-stage kidney failure. The discovery that the kidney is the site of production of calcitriol clarifies our understanding of calcium metabolic disorders in chronic renal failure and carries with it clinical implications in the treatment of renal rickets. The author presents data on the action of calcium and reviews the therapeutic effects on uremic osteodystrophy in children of calcitriol and its analogue."}
{"id": "PMID:463256", "title": "Family planning in Virginia: the role of public health.", "content": "Under the aegis of many medical organizations, family planning in Virginia has grown remarkably in recent years. Today the State Department of Health administers a statewide program to foster family planning and has recently created a voluntary sterilization fund, the first of its kind in the nation.", "contents": "Family planning in Virginia: the role of public health. Under the aegis of many medical organizations, family planning in Virginia has grown remarkably in recent years. Today the State Department of Health administers a statewide program to foster family planning and has recently created a voluntary sterilization fund, the first of its kind in the nation."}
{"id": "PMID:463261", "title": "Viral hepatitis: prevention and prophylaxis.", "content": "Hepatitis A is spread by fecal-oral transmission and accounts for 25% of the cases of sporadic hepatitis in this country; fatal cases have been documented but are unusual, and chronic hepatitis A has not been documented. Hepatitis B is spread by varied routes of transmission, fecal-oral being the least important, and accounts for half the cases of sporadic hepatitis in this country; fatal cases are well documented and chronic hepatitis B is common. The only documented route of transmission of non-A, non-B hepatitis is parenteral; fatal and chronic cases have been documented, and a quarter of the cases of sporadic hepatitis are non-A, non-B. Diagnosis of the etiology of viral hepatitis cannot be determined on clinical grounds but must be made through serologic tests.", "contents": "Viral hepatitis: prevention and prophylaxis. Hepatitis A is spread by fecal-oral transmission and accounts for 25% of the cases of sporadic hepatitis in this country; fatal cases have been documented but are unusual, and chronic hepatitis A has not been documented. Hepatitis B is spread by varied routes of transmission, fecal-oral being the least important, and accounts for half the cases of sporadic hepatitis in this country; fatal cases are well documented and chronic hepatitis B is common. The only documented route of transmission of non-A, non-B hepatitis is parenteral; fatal and chronic cases have been documented, and a quarter of the cases of sporadic hepatitis are non-A, non-B. Diagnosis of the etiology of viral hepatitis cannot be determined on clinical grounds but must be made through serologic tests."}
{"id": "PMID:463262", "title": "Colonoscopy in a small community hospital.", "content": "Colonoscopy is seldom performed in a small community hospital, due to the high cost of instruments and other factors but the author has accomplished 450 colonoscopies in that setting, with a small complication rate and no mortality.", "contents": "Colonoscopy in a small community hospital. Colonoscopy is seldom performed in a small community hospital, due to the high cost of instruments and other factors but the author has accomplished 450 colonoscopies in that setting, with a small complication rate and no mortality."}
{"id": "PMID:463263", "title": "Early control of a community measles outbreak.", "content": "Door-to-door immunizations and a community canvass for susceptibles were marshalled to quell a rubeola outbreak in Norfolk, one of 25 outbreaks reported in Virginia from January through August 1977.", "contents": "Early control of a community measles outbreak. Door-to-door immunizations and a community canvass for susceptibles were marshalled to quell a rubeola outbreak in Norfolk, one of 25 outbreaks reported in Virginia from January through August 1977."}
{"id": "PMID:463276", "title": "Computed tomography of the pancreas: a preliminary experience.", "content": "CT appears to be an excellent non-invasive method for the evaluation of pancreatic disease, particularly when ultrasonography is inconclusive. The authors review their preliminary experience and analyze the results in 34 patients.", "contents": "Computed tomography of the pancreas: a preliminary experience. CT appears to be an excellent non-invasive method for the evaluation of pancreatic disease, particularly when ultrasonography is inconclusive. The authors review their preliminary experience and analyze the results in 34 patients."}
{"id": "PMID:463277", "title": "Microsurgical treatment of intracranial aneurysms.", "content": "An important advance in aneurysm surgery is the operating microscope, which allows the neurosurgeon access to previously inoperable lesions. The authors describe the technique and document their results in 19 aneurysms.", "contents": "Microsurgical treatment of intracranial aneurysms. An important advance in aneurysm surgery is the operating microscope, which allows the neurosurgeon access to previously inoperable lesions. The authors describe the technique and document their results in 19 aneurysms."}
{"id": "PMID:463278", "title": "Nissen fundoplication.", "content": "A personal series of 16 patients treated with Nissen fundoplication for reflux peptic esophagitis with or without hiatal hernia between March 1975 and September 1977 is presented. The average length of followup was 18 months. The age range was 21 to 78. Very good to excellent results were achieved in 94% with a 12.5% morbidity, 6% operative complication rate and 0 mortality. This relatively simple procedure definitely reduces, if not cures, the symptomatology of the often \"miserable\" patient with intractable reflux peptic esophagitis. The encouraging reports in the recent literature concerning patients treated with this operation recommend its wider use in the future.", "contents": "Nissen fundoplication. A personal series of 16 patients treated with Nissen fundoplication for reflux peptic esophagitis with or without hiatal hernia between March 1975 and September 1977 is presented. The average length of followup was 18 months. The age range was 21 to 78. Very good to excellent results were achieved in 94% with a 12.5% morbidity, 6% operative complication rate and 0 mortality. This relatively simple procedure definitely reduces, if not cures, the symptomatology of the often \"miserable\" patient with intractable reflux peptic esophagitis. The encouraging reports in the recent literature concerning patients treated with this operation recommend its wider use in the future."}
{"id": "PMID:463347", "title": "Toxic trace metals in food. II. A comparative study of the levels of toxic trace metals in wine by differential pulse anodic stripping voltammetry and electrothermal atomic absorption spectrometry.", "content": "A new high-performance analytical procedure for the determination of the toxic trace metals cadmium, lead and copper in wines by differential pulse anodic-stripping voltammetry (DPASV) subsequent to UV irradiation of the sample is compared with the hitherto more common application of electrothermal atomic absorption spectrometry (AAS). In this manner also mutually the accuracy attainable with both alternatives has been established. The particularly favourable potentialities of the new voltammetric approach for toxic metal control of wines are demonstrated by the investigation of a typical selection of 36 wines from recent vintages and common vine types of the German and some European wine cultivating regions.", "contents": "Toxic trace metals in food. II. A comparative study of the levels of toxic trace metals in wine by differential pulse anodic stripping voltammetry and electrothermal atomic absorption spectrometry. A new high-performance analytical procedure for the determination of the toxic trace metals cadmium, lead and copper in wines by differential pulse anodic-stripping voltammetry (DPASV) subsequent to UV irradiation of the sample is compared with the hitherto more common application of electrothermal atomic absorption spectrometry (AAS). In this manner also mutually the accuracy attainable with both alternatives has been established. The particularly favourable potentialities of the new voltammetric approach for toxic metal control of wines are demonstrated by the investigation of a typical selection of 36 wines from recent vintages and common vine types of the German and some European wine cultivating regions."}
{"id": "PMID:463350", "title": "[Health planning and management, instruments for technical cooperation].", "content": "Western models of health strategy are not applicable in developing countries. These need, for developing original and effective health services and systems, a planning method meeting their specific requirements. A WHO-sponsored collaborative development effort from 1970 to this day resulted in a set of programming, project formulation and management procedures broadly based on the systems approach. The 31st World Health Assembly commended this effort, which is on-going.", "contents": "[Health planning and management, instruments for technical cooperation]. Western models of health strategy are not applicable in developing countries. These need, for developing original and effective health services and systems, a planning method meeting their specific requirements. A WHO-sponsored collaborative development effort from 1970 to this day resulted in a set of programming, project formulation and management procedures broadly based on the systems approach. The 31st World Health Assembly commended this effort, which is on-going."}
{"id": "PMID:463352", "title": "[Swiss cooperation in the development of public health in Tanzania].", "content": "With the financial support of the Basle Foundation for Aid to Developing Countries and in close collaboration with the St. Francis hospital, the Swiss Tropical Institute has set up and looked after a teaching centre at Ifakara, Tanzania, for the training of African medical, paramedical and auxiliary personnel. In the course of seventeen years, 770 young Africans have undergone training here. The centre has now been handed over in its final form as a Medical Assistants Training Centre to the Tanzanian Government, which has assumed full responsibility for its further direction and maintenance. The results obtained and the experience gained are discussed, as is the relationship of the centre to the public health policy of the Tanzanian Government. The writer considers that the whole undertaking has been of great mutual benefit and is looking forward to further collaboration between the Swiss Tropical Institute. Based on the Field Laboratory Ifakara, this will be mainly in applied epidemiological research, e.g. on schistosomiasis in and around Ifakara.", "contents": "[Swiss cooperation in the development of public health in Tanzania]. With the financial support of the Basle Foundation for Aid to Developing Countries and in close collaboration with the St. Francis hospital, the Swiss Tropical Institute has set up and looked after a teaching centre at Ifakara, Tanzania, for the training of African medical, paramedical and auxiliary personnel. In the course of seventeen years, 770 young Africans have undergone training here. The centre has now been handed over in its final form as a Medical Assistants Training Centre to the Tanzanian Government, which has assumed full responsibility for its further direction and maintenance. The results obtained and the experience gained are discussed, as is the relationship of the centre to the public health policy of the Tanzanian Government. The writer considers that the whole undertaking has been of great mutual benefit and is looking forward to further collaboration between the Swiss Tropical Institute. Based on the Field Laboratory Ifakara, this will be mainly in applied epidemiological research, e.g. on schistosomiasis in and around Ifakara."}
{"id": "PMID:463353", "title": "[Training of physicians at the Swiss Tropical Institute].", "content": "On account of the evolution of the concept of health care in developing countries, the author describes the new orientation of the medical training at the Swiss Tropical Institute. For this purpose, the miscellaneous problems met, as well as their solutions, are presented. The training of physicians in tropical medicine is more and more taking the shape of a preparation to \"development medicine\".", "contents": "[Training of physicians at the Swiss Tropical Institute]. On account of the evolution of the concept of health care in developing countries, the author describes the new orientation of the medical training at the Swiss Tropical Institute. For this purpose, the miscellaneous problems met, as well as their solutions, are presented. The training of physicians in tropical medicine is more and more taking the shape of a preparation to \"development medicine\"."}
{"id": "PMID:463354", "title": "[An example of the application of factorial analysis of correspondences to infant mortality and its prevention in a rural area of West Africa].", "content": "A retrospective study through questionnaire was made among over 800 women of a Pre-Sahel region of Mali. It confirmed the very high infant and child mortality in this population: over 250% mortality under 1 year of age and over 400% under 6 years of age. The factorial analysis of correspondences proved adequate to determine the main characteristics of the local mortality pattern. In chronological order, the most important causes are obstetrical factors (1st day of life), umbilical tetanus (1 week to 1 month of age), malaria (1 month to 1 year of age), pneumopathies (including pertussis and lung complications of measles), toxicoses and nutritional syndromes (over 1 year of age).  Preventive measures at various levels are proposed on the basis of these findings. They belong to three broad groups: -Overall socioeconomic development with effective participation of the rural communities concerned. -Development of an appropriate primary health care structure, with a primary health care team in each village. -Strengthening of the programmes of control of the most important communicable diseases. Such simple and cheap actions should, given a clear political will of the national authorities and a modicum of trust in the future on the part of local leaders, be rapidly implementable even in the most underprivileged countries and bring a significant amount of progress in the rural areas.", "contents": "[An example of the application of factorial analysis of correspondences to infant mortality and its prevention in a rural area of West Africa]. A retrospective study through questionnaire was made among over 800 women of a Pre-Sahel region of Mali. It confirmed the very high infant and child mortality in this population: over 250% mortality under 1 year of age and over 400% under 6 years of age. The factorial analysis of correspondences proved adequate to determine the main characteristics of the local mortality pattern. In chronological order, the most important causes are obstetrical factors (1st day of life), umbilical tetanus (1 week to 1 month of age), malaria (1 month to 1 year of age), pneumopathies (including pertussis and lung complications of measles), toxicoses and nutritional syndromes (over 1 year of age).  Preventive measures at various levels are proposed on the basis of these findings. They belong to three broad groups: -Overall socioeconomic development with effective participation of the rural communities concerned. -Development of an appropriate primary health care structure, with a primary health care team in each village. -Strengthening of the programmes of control of the most important communicable diseases. Such simple and cheap actions should, given a clear political will of the national authorities and a modicum of trust in the future on the part of local leaders, be rapidly implementable even in the most underprivileged countries and bring a significant amount of progress in the rural areas."}
{"id": "PMID:463355", "title": "[Integration of health development cooperation into the cultural and socioeconomic context of the operational area: work of the Putina medical team in Peru].", "content": "Contrasting with what happened with the indio traditional medicine and with the Western medical knowledge the Spaniards imported, industrialized nations' medicine, through its growing ability to fight infectious diseases, has sharpened in Peru in the 20th century the social disparity between the well-to-do upper classes and the poor masses. In order to alleviate this situation, efforts have been undertaken since the 1940's in the Department of Puno, the poorest of the country, in order to bring to the rural population the benefits of modern medicine. Since 1971, a team of Swiss physicians, integrated into the Peruvian Ministry of Health, works in the Putina area in several State health centers. There are presently five doctors for a 130,000 inhabitants population, collaborating in the setting up of basic health services. The most important pillars of the programme, which should be able to function without physicians, if need be, are voluntary health auxiliaries, chosen by their own community (1 per 500 people). State employed auxiliary nurses (1 per 5000 people), and Peruvian State Registered nurses, entrusted with training and supervisory tasks.  The progress of the programme, in terms of the growing independence of the basic health system, is described, as are potentialities and limits in bringing it closer to the indigenous traditional medicine. Through the example of the supply of basic drugs, it is pointed out how setbacks suffered in the last two years, are illustrative of the country political crisis and of its growing dependency on industrialized nations.", "contents": "[Integration of health development cooperation into the cultural and socioeconomic context of the operational area: work of the Putina medical team in Peru]. Contrasting with what happened with the indio traditional medicine and with the Western medical knowledge the Spaniards imported, industrialized nations' medicine, through its growing ability to fight infectious diseases, has sharpened in Peru in the 20th century the social disparity between the well-to-do upper classes and the poor masses. In order to alleviate this situation, efforts have been undertaken since the 1940's in the Department of Puno, the poorest of the country, in order to bring to the rural population the benefits of modern medicine. Since 1971, a team of Swiss physicians, integrated into the Peruvian Ministry of Health, works in the Putina area in several State health centers. There are presently five doctors for a 130,000 inhabitants population, collaborating in the setting up of basic health services. The most important pillars of the programme, which should be able to function without physicians, if need be, are voluntary health auxiliaries, chosen by their own community (1 per 500 people). State employed auxiliary nurses (1 per 5000 people), and Peruvian State Registered nurses, entrusted with training and supervisory tasks.  The progress of the programme, in terms of the growing independence of the basic health system, is described, as are potentialities and limits in bringing it closer to the indigenous traditional medicine. Through the example of the supply of basic drugs, it is pointed out how setbacks suffered in the last two years, are illustrative of the country political crisis and of its growing dependency on industrialized nations."}
{"id": "PMID:463356", "title": "[The voluntary health auxiliary. Application to the Peruvian Altiplano of new concepts of primary health care in disadvantaged communities].", "content": "A group a Swiss physicians, who have been working for about eight years in rural health posts of the Peruvian highlands (Altiplano), engaged in training voluntary health auxiliaries, chosen by the indigenous communities. Experience has shown that, as in other countries, it is thus possible to markedly improve the health of populations which badly lack resources. The efficacy of using such auxiliaries was recognized by the Peruvian Ministry of Health, which has now set up a programme for their training and supervision. Within the concept of primary health care, based on the participation of people to the management of their health, the voluntary health auxiliary is placed in a strategic and decisive position for the success of an enterprise which is now spreading to many countries of the Third World.", "contents": "[The voluntary health auxiliary. Application to the Peruvian Altiplano of new concepts of primary health care in disadvantaged communities]. A group a Swiss physicians, who have been working for about eight years in rural health posts of the Peruvian highlands (Altiplano), engaged in training voluntary health auxiliaries, chosen by the indigenous communities. Experience has shown that, as in other countries, it is thus possible to markedly improve the health of populations which badly lack resources. The efficacy of using such auxiliaries was recognized by the Peruvian Ministry of Health, which has now set up a programme for their training and supervision. Within the concept of primary health care, based on the participation of people to the management of their health, the voluntary health auxiliary is placed in a strategic and decisive position for the success of an enterprise which is now spreading to many countries of the Third World."}
{"id": "PMID:463357", "title": "[Preventive aspects and problems of a rural health project in Chad--the need for health motivation].", "content": "Some of the main difficulties encountered in health work in developing countries depend on the sociocultural conditions. Health animatiod until now) of rural health care. Its implementation requires the training and recycling of existing health personnel as well as of parts of the population. It includes the concept of \"barefoot doctor\", which corresponds well to the prevailing priority health needs. One should note potential difficulties, however, for example in regard to the way the barefoot doctor is to be remunerated (the author discusses some possibilities). It isn't easy either, in practice, to mobilize the community through health education and to obtain its participation in prevention and health promotion measures. The crucial element is to win its confidence. The author describes his practical experiences in Chad in this respect. Health animation stimulates the responsabilization and the development of a prospective outlook in people, and can thus make an important contribution to their overall development efforts. It is necessary to fight factors which slow down such an evolution, among which certain habits as well as obstacles of an administrative nature.", "contents": "[Preventive aspects and problems of a rural health project in Chad--the need for health motivation]. Some of the main difficulties encountered in health work in developing countries depend on the sociocultural conditions. Health animatiod until now) of rural health care. Its implementation requires the training and recycling of existing health personnel as well as of parts of the population. It includes the concept of \"barefoot doctor\", which corresponds well to the prevailing priority health needs. One should note potential difficulties, however, for example in regard to the way the barefoot doctor is to be remunerated (the author discusses some possibilities). It isn't easy either, in practice, to mobilize the community through health education and to obtain its participation in prevention and health promotion measures. The crucial element is to win its confidence. The author describes his practical experiences in Chad in this respect. Health animation stimulates the responsabilization and the development of a prospective outlook in people, and can thus make an important contribution to their overall development efforts. It is necessary to fight factors which slow down such an evolution, among which certain habits as well as obstacles of an administrative nature."}
{"id": "PMID:463358", "title": "[Treatment of fractures by traditional healers in Morocco].", "content": "Traditional traumatologists, specialized in the treatment of fractures, practise throughout Morocco. Their original technique is described and its efficiency evaluated through twenty or so cases studied during three years of medical work in the Great South of the country. Theories are propounded to explain the population's preference for these treatments to the detriment of the medical methods, which have a bad reputation. In the present situation, the medical infrastructure is unable to replace the benefits of these bonesetters, neither in quality--with the exception of the main centres-nor, above all, in quantity. Their activities are decentralized, socially integrated and economical for both the population and the state. These considerations lead to the conclusion that their practice should be maintained, improving it if need be.", "contents": "[Treatment of fractures by traditional healers in Morocco]. Traditional traumatologists, specialized in the treatment of fractures, practise throughout Morocco. Their original technique is described and its efficiency evaluated through twenty or so cases studied during three years of medical work in the Great South of the country. Theories are propounded to explain the population's preference for these treatments to the detriment of the medical methods, which have a bad reputation. In the present situation, the medical infrastructure is unable to replace the benefits of these bonesetters, neither in quality--with the exception of the main centres-nor, above all, in quantity. Their activities are decentralized, socially integrated and economical for both the population and the state. These considerations lead to the conclusion that their practice should be maintained, improving it if need be."}
{"id": "PMID:463361", "title": "[Medical development cooperation in the Confederation. The international framework and the important place of public health in Swiss development cooperation].", "content": "The systematic organization of health services in the developing countries to benefit the majority of the population is seen as an important contribution toward the general economic and social development of these countries. This view is uncontested today, but was given less importance in the early years of Swiss development aid. The Swiss Development Cooperation's support of health services is now integrated into its overall program to satisfy the basic needs of the people of the developing countries.", "contents": "[Medical development cooperation in the Confederation. The international framework and the important place of public health in Swiss development cooperation]. The systematic organization of health services in the developing countries to benefit the majority of the population is seen as an important contribution toward the general economic and social development of these countries. This view is uncontested today, but was given less importance in the early years of Swiss development aid. The Swiss Development Cooperation's support of health services is now integrated into its overall program to satisfy the basic needs of the people of the developing countries."}
{"id": "PMID:463362", "title": "[Swiss Confederation cooperation in health development: beginnings, experiences and present projects].", "content": "The types of medical and health activities in the first ten years of the Swiss Confederation's development aid program (1961-71) are examined in a critical light. In that period such aid was primarily in support of missionary hospitals in Africa and came to 3% of the total aid program of Switzerland. In the seventies guidelines for health programs were established and these have since been systematically applied. The principal aspects are: creation of basic health services, adaptation to local conditions, integration into the overall economic and social development efforts, training of health personnel. In conclusion, the article lists geographically those health projects, which are at present financed and/or staffed by DEH.", "contents": "[Swiss Confederation cooperation in health development: beginnings, experiences and present projects]. The types of medical and health activities in the first ten years of the Swiss Confederation's development aid program (1961-71) are examined in a critical light. In that period such aid was primarily in support of missionary hospitals in Africa and came to 3% of the total aid program of Switzerland. In the seventies guidelines for health programs were established and these have since been systematically applied. The principal aspects are: creation of basic health services, adaptation to local conditions, integration into the overall economic and social development efforts, training of health personnel. In conclusion, the article lists geographically those health projects, which are at present financed and/or staffed by DEH."}
{"id": "PMID:463363", "title": "[Fr\u00e8res des Hommes--actions with grass roots participation aimed at satisfying priority needs: from theory to practice].", "content": "Brothers of All Men (Fr\u00e8res des Hommes) is a group of the two objectives of which are concrete direct action in the Third World, where the underdevelopment situation is most crucial, and an information and sensitization action in Europe. The primary health-care concept is fashionable presently, and this is all well. In the light of Brothers of All Men's practical experience in the field, at the grass roots, and on several continents, the author, however, wants to call attention, among the current enthusiasm, to serious difficulties, and certain failures, encountered in primary health-care projects undertaken with the participation of the concerned communities. He does it in particular on the basis of efforts in Sahelian Africa and Bangladesh. He also recalls that it is not the Third World partner who is supposed to assist in pursuing the goals of a foreign cooperation agency, but the latter which proposes its collaboration to the former. Consquently, projects must be formulated by the local communities and controlled by them. The implementation of primary health-care programmes requires the conjunction of two flux, a real popular dynamics locally, at the periphery, and a clearly defined national political will. It appears that, for the time being, those conditions are more easily found in congress halls than in the field.", "contents": "[Fr\u00e8res des Hommes--actions with grass roots participation aimed at satisfying priority needs: from theory to practice]. Brothers of All Men (Fr\u00e8res des Hommes) is a group of the two objectives of which are concrete direct action in the Third World, where the underdevelopment situation is most crucial, and an information and sensitization action in Europe. The primary health-care concept is fashionable presently, and this is all well. In the light of Brothers of All Men's practical experience in the field, at the grass roots, and on several continents, the author, however, wants to call attention, among the current enthusiasm, to serious difficulties, and certain failures, encountered in primary health-care projects undertaken with the participation of the concerned communities. He does it in particular on the basis of efforts in Sahelian Africa and Bangladesh. He also recalls that it is not the Third World partner who is supposed to assist in pursuing the goals of a foreign cooperation agency, but the latter which proposes its collaboration to the former. Consquently, projects must be formulated by the local communities and controlled by them. The implementation of primary health-care programmes requires the conjunction of two flux, a real popular dynamics locally, at the periphery, and a clearly defined national political will. It appears that, for the time being, those conditions are more easily found in congress halls than in the field."}
{"id": "PMID:463367", "title": "[Kinetics of the growth of Ca oxalate crystals from supersaturated solutions].", "content": "Several factors influencing the nucleation and growth of Ca-oxalate crystals from metastable and instable solutions were studied in some detail. Factors of interest were the absolute concentration of calcium respectively oxalate, the quotient oxalate/calcium, repeated additions of calcium and/or oxalate, the presence or absence of crystal seeds, the agitation respectively stagnation of the metastable Ca-oxalate solution, the duration of crystallization, etc. The striking findings are the eminent role of oxalate in the formation of big crystals and crystal aggregates, the distinct inhibition of crystal growth at higher and very high calcium concentrations, as well as the substantial crystal enlargement at the presistent oxalate load.", "contents": "[Kinetics of the growth of Ca oxalate crystals from supersaturated solutions]. Several factors influencing the nucleation and growth of Ca-oxalate crystals from metastable and instable solutions were studied in some detail. Factors of interest were the absolute concentration of calcium respectively oxalate, the quotient oxalate/calcium, repeated additions of calcium and/or oxalate, the presence or absence of crystal seeds, the agitation respectively stagnation of the metastable Ca-oxalate solution, the duration of crystallization, etc. The striking findings are the eminent role of oxalate in the formation of big crystals and crystal aggregates, the distinct inhibition of crystal growth at higher and very high calcium concentrations, as well as the substantial crystal enlargement at the presistent oxalate load."}
{"id": "PMID:463368", "title": "[Ionic calcium in blood and urine. Methods of determination and methodological difficulties].", "content": "The present paper reports about the individual fractions of the plasma and urine calcium, further about the analytical methods for the determination of the ionic Ca, as well as about the methodical difficulties of the described analytical techniques. Considerations about the real informative value of the Ca2+ -determination in the urine led us to the conclusion that only the additional knowledge of the actual concentration of the stone-forming anions, the oxalate resp. the phosphate may mediate a more complete picture about the calculogenetic \"aggressivity\" of the urine under investigation.", "contents": "[Ionic calcium in blood and urine. Methods of determination and methodological difficulties]. The present paper reports about the individual fractions of the plasma and urine calcium, further about the analytical methods for the determination of the ionic Ca, as well as about the methodical difficulties of the described analytical techniques. Considerations about the real informative value of the Ca2+ -determination in the urine led us to the conclusion that only the additional knowledge of the actual concentration of the stone-forming anions, the oxalate resp. the phosphate may mediate a more complete picture about the calculogenetic \"aggressivity\" of the urine under investigation."}
{"id": "PMID:463369", "title": "[Compliance and active cooperation of patients in preventing recurrence of urinary calculi].", "content": "An anonymous questioning of 200 patients with urinary calculi concerning the observation of the general and special measures of the metaphylaxis of urinary calculi resulted in the fact that in particular the measures of the general metaphylaxis (bodily movement, diet, dilution of the urine) are not or only parlty obeyed by a large part of the patients. The examinations show that for the improvement of the understanding of the disease and the behaviour of the patients concerning their health further on a great attension must be paid to the quality of the information by the physician and to the communication between physician and patient. An insufficient cooperation of the patients in the metaphylaxis of the urinary calculi would call in question the effectivity of all efforts and measures concerning the prevention of relapses.", "contents": "[Compliance and active cooperation of patients in preventing recurrence of urinary calculi]. An anonymous questioning of 200 patients with urinary calculi concerning the observation of the general and special measures of the metaphylaxis of urinary calculi resulted in the fact that in particular the measures of the general metaphylaxis (bodily movement, diet, dilution of the urine) are not or only parlty obeyed by a large part of the patients. The examinations show that for the improvement of the understanding of the disease and the behaviour of the patients concerning their health further on a great attension must be paid to the quality of the information by the physician and to the communication between physician and patient. An insufficient cooperation of the patients in the metaphylaxis of the urinary calculi would call in question the effectivity of all efforts and measures concerning the prevention of relapses."}
{"id": "PMID:463370", "title": "[Neurogenic bladder dysfunction in childhood. I. Diagnosis].", "content": "The diagnostic demands of the neurogenic disturbances of the voiding of the bladder in patients with myelodysplasia are summarized in tabulated form. It is reported in detail on own experiences of the authors with urodynamic measuring methods and with the electromyography of the striated musculature of the floor of the pelvis. At present the two methods are not yet used everywhere and they are a considerable enrichment of the diagnostics.", "contents": "[Neurogenic bladder dysfunction in childhood. I. Diagnosis]. The diagnostic demands of the neurogenic disturbances of the voiding of the bladder in patients with myelodysplasia are summarized in tabulated form. It is reported in detail on own experiences of the authors with urodynamic measuring methods and with the electromyography of the striated musculature of the floor of the pelvis. At present the two methods are not yet used everywhere and they are a considerable enrichment of the diagnostics."}
{"id": "PMID:463372", "title": "[Evaluation of hydroxyproline excretion in the urine (UHP) in prostate carcinoma].", "content": "82% of the male patients with healthy urogenital tract showed an isoelectric line in the stimulataneous EMG-derivation of the sphincter of the bladder under conditions of rest; in 18% of the males a moderate activity was present. In voluntary innervation at once an intensive electric activity of a spinle-shaped discontinuous pattern of the action potential developed in 76% of the patients. In the pressing trial in 83% of the cases a slight to moderately strong activity appeared. In contrast to the examined patients with healthy outlet of the vesicle the patients in whom an adenoma of the prostate gland or a carcinoma of the prostate gland, respectively, was present exhibited deviations from the normal pattern of the action potential, in which cases carriers of adenoma and carcinoma differed. Whether and to what extent the electromyography on the m. sphincter vesicae allows the possibility of the differentiation between adenoma of the prostate gland and carcinomaof the prostate gland in the early clinical stage shall be reserved for further examination.", "contents": "[Evaluation of hydroxyproline excretion in the urine (UHP) in prostate carcinoma]. 82% of the male patients with healthy urogenital tract showed an isoelectric line in the stimulataneous EMG-derivation of the sphincter of the bladder under conditions of rest; in 18% of the males a moderate activity was present. In voluntary innervation at once an intensive electric activity of a spinle-shaped discontinuous pattern of the action potential developed in 76% of the patients. In the pressing trial in 83% of the cases a slight to moderately strong activity appeared. In contrast to the examined patients with healthy outlet of the vesicle the patients in whom an adenoma of the prostate gland or a carcinoma of the prostate gland, respectively, was present exhibited deviations from the normal pattern of the action potential, in which cases carriers of adenoma and carcinoma differed. Whether and to what extent the electromyography on the m. sphincter vesicae allows the possibility of the differentiation between adenoma of the prostate gland and carcinomaof the prostate gland in the early clinical stage shall be reserved for further examination."}
{"id": "PMID:463373", "title": "[Lymphogenic metastasis in prostate carcinoma and radiation therapy].", "content": "Our examinations allow the following conclusions: 1. Before the beginning of a curative radiotherapy of the carcinoma of the prostate gland in a negative and questionably positive lymphogramme the pelvic lymphadenectomy with histologic examination of the lymph nodes is indicated. 2. In the negative findings of the histologically examined lymph nodes an exclusive local tumour irradiation is justified. 3. If in regional and negative findings micrometastases appear in the area of juxtaregional lymph nodes the additional curative irradiation of the pelvic lymph nodes seems to be indicated for the comprehension of the regional lymph nodes. 4. When there are micrometastases in the area of the aa. iliacae communes or macroscopically extended metastases in the regional and juxtaregional lymph area, so we regard as a rule a radiotherapy of the carcinoma of the prostate gland no more indicated and recommend a contrasexual treatment. The necessary expansion of the radiotherapy is according to our hitherto existing experiences badly tolerated by the for the most part older patients. 5. The intraoperative labelling of the prostate gland and the determination of the size of the prostate gland facilitate the localisation of the tumour for the radiotherapist.", "contents": "[Lymphogenic metastasis in prostate carcinoma and radiation therapy]. Our examinations allow the following conclusions: 1. Before the beginning of a curative radiotherapy of the carcinoma of the prostate gland in a negative and questionably positive lymphogramme the pelvic lymphadenectomy with histologic examination of the lymph nodes is indicated. 2. In the negative findings of the histologically examined lymph nodes an exclusive local tumour irradiation is justified. 3. If in regional and negative findings micrometastases appear in the area of juxtaregional lymph nodes the additional curative irradiation of the pelvic lymph nodes seems to be indicated for the comprehension of the regional lymph nodes. 4. When there are micrometastases in the area of the aa. iliacae communes or macroscopically extended metastases in the regional and juxtaregional lymph area, so we regard as a rule a radiotherapy of the carcinoma of the prostate gland no more indicated and recommend a contrasexual treatment. The necessary expansion of the radiotherapy is according to our hitherto existing experiences badly tolerated by the for the most part older patients. 5. The intraoperative labelling of the prostate gland and the determination of the size of the prostate gland facilitate the localisation of the tumour for the radiotherapist."}
{"id": "PMID:463375", "title": "[Complications of urologic diseases in diabetes mellitus].", "content": "It is reported on complications of urological diseases in diabetes mellitus. Above all the diabetic late syndrome and its multiform and uncharacteristic symptomatology in the urogenital system are discussed in detail. The present form of diabetes control can not yet be regarded as optimum form. It should be striven for changes, since it is to be reckoned with an increase of diabetes mellitus all over the world.", "contents": "[Complications of urologic diseases in diabetes mellitus]. It is reported on complications of urological diseases in diabetes mellitus. Above all the diabetic late syndrome and its multiform and uncharacteristic symptomatology in the urogenital system are discussed in detail. The present form of diabetes control can not yet be regarded as optimum form. It should be striven for changes, since it is to be reckoned with an increase of diabetes mellitus all over the world."}
{"id": "PMID:463376", "title": "[Adenomectomy in hemophilia A patients].", "content": "A patient with haemophilia A is described in whom a loading blood-loss after adenomectomy developed. The congenital haemorrhagical diathesis was diagnosed only after the surgical intervention, since there did not exist any clinical symptoms. Taking into consideration world literature the problem of adenomectomy in patients with haemophilic is discussed.", "contents": "[Adenomectomy in hemophilia A patients]. A patient with haemophilia A is described in whom a loading blood-loss after adenomectomy developed. The congenital haemorrhagical diathesis was diagnosed only after the surgical intervention, since there did not exist any clinical symptoms. Taking into consideration world literature the problem of adenomectomy in patients with haemophilic is discussed."}
{"id": "PMID:463377", "title": "[Multicentricity of urothelial tumors of the efferent urinary tracts].", "content": "On the basis of 8 cases with primary urothelial tumours of the upper efferent urinary tract with simultaneously only partly preoperatively diagnosed secondary tumours of the urothelium the problems of diagnostics and therapy are discussed. A conservative surgery may be regarded as sufficiently radical only then when a multicentric genesis may be excluded with certainty. In our patients with tumours of the upper efferent urinary tract we found a multicentricity in 18.4%.", "contents": "[Multicentricity of urothelial tumors of the efferent urinary tracts]. On the basis of 8 cases with primary urothelial tumours of the upper efferent urinary tract with simultaneously only partly preoperatively diagnosed secondary tumours of the urothelium the problems of diagnostics and therapy are discussed. A conservative surgery may be regarded as sufficiently radical only then when a multicentric genesis may be excluded with certainty. In our patients with tumours of the upper efferent urinary tract we found a multicentricity in 18.4%."}
{"id": "PMID:463385", "title": "[Primary nodular amyloidosis of the lung (author's transl)].", "content": "A 69-year old woman deceased from a pericardial tamponade following a myocardial infarction. In the autopsy, approximately 30 cherry-sized or smaller nodules, were unexpectedly found in the subpleural tissue. These nodules produced no clinical manifestations, however histological and polarisation optical investigations showed that they correspond to an isolated primary pericollagenous amyloidosis of the lung.", "contents": "[Primary nodular amyloidosis of the lung (author's transl)]. A 69-year old woman deceased from a pericardial tamponade following a myocardial infarction. In the autopsy, approximately 30 cherry-sized or smaller nodules, were unexpectedly found in the subpleural tissue. These nodules produced no clinical manifestations, however histological and polarisation optical investigations showed that they correspond to an isolated primary pericollagenous amyloidosis of the lung."}
{"id": "PMID:463386", "title": "[Two cases with vitium cordis congenitum and rare nodules in the epicard (author's transl)].", "content": "In one newborn and one infant with vitium cordis congenitum in the epicard in correlation with coronary arteries little nodules were found. By histological observation a direct communication of large intermyocardial sinusoids and the lumen of the ventricle was seen. It is suggested, that these are arterio-venous anastomoses.", "contents": "[Two cases with vitium cordis congenitum and rare nodules in the epicard (author's transl)]. In one newborn and one infant with vitium cordis congenitum in the epicard in correlation with coronary arteries little nodules were found. By histological observation a direct communication of large intermyocardial sinusoids and the lumen of the ventricle was seen. It is suggested, that these are arterio-venous anastomoses."}
{"id": "PMID:463387", "title": "[Peritoneal aluminosis after alugramin treatment of decubital ulcers (author's transl)].", "content": "The occurrence of unusual peritoneal and subserous deposits of metallic aluminum granules in a 34 years old man after local alugramin treatment of decubital ulcers lasting for about 10 weeks is described. He died of an apallic syndrome approximately 3 months after a head-brain-injury due to an accident and terminal bronchopneumonia. The peritoneal aluminosis was diagnosed at autopsy as a secondary finding. An inflammatory peritoneal reaction was not notified. The lymphatic ganglion system is assumed to be the route of transport.", "contents": "[Peritoneal aluminosis after alugramin treatment of decubital ulcers (author's transl)]. The occurrence of unusual peritoneal and subserous deposits of metallic aluminum granules in a 34 years old man after local alugramin treatment of decubital ulcers lasting for about 10 weeks is described. He died of an apallic syndrome approximately 3 months after a head-brain-injury due to an accident and terminal bronchopneumonia. The peritoneal aluminosis was diagnosed at autopsy as a secondary finding. An inflammatory peritoneal reaction was not notified. The lymphatic ganglion system is assumed to be the route of transport."}
{"id": "PMID:463388", "title": "[Contribution to the knowledge of the \"periostitis ossificans interna\" of the cranial vault (author's transl)].", "content": "An unusual observation of hyperostosis of the frontal bone exhibiting five alternating layers of compact and cancellous bone on cross section is demonstrated. The peculiar, hitherto not described hyperostosis occurred in an 18 year old female suffering from recurrent chronical pachy- and lepto-meningitis since birth. A second similar observation was made in a 15 year old male, trepanated because of an epidural abscess. Similar accessory layers of cancellous bone on the internal surface of temporal bone suggested that also the first observation had to be interpreted as an unusually extensive form of periostitis ossificans, only involving the frontal bone, due to the recurring inflammation processes of the meninges.", "contents": "[Contribution to the knowledge of the \"periostitis ossificans interna\" of the cranial vault (author's transl)]. An unusual observation of hyperostosis of the frontal bone exhibiting five alternating layers of compact and cancellous bone on cross section is demonstrated. The peculiar, hitherto not described hyperostosis occurred in an 18 year old female suffering from recurrent chronical pachy- and lepto-meningitis since birth. A second similar observation was made in a 15 year old male, trepanated because of an epidural abscess. Similar accessory layers of cancellous bone on the internal surface of temporal bone suggested that also the first observation had to be interpreted as an unusually extensive form of periostitis ossificans, only involving the frontal bone, due to the recurring inflammation processes of the meninges."}
{"id": "PMID:463389", "title": "[Familial occurrence of onocytic carcinoma of the thyroid gland during pregnancy (author's transl)].", "content": "Two cases of oncocytic papillary carcinoma of the thyroid gland are reported, which occurred in two sisters during pregnancy. In both cases the follow-up is without complications (after strumectomy and postoperative radiation) and therefore it is in strong contrast to the aggressive biological behaviour during pregnancy. The oncocytic papillary carcinoma is a rare disease in the endemic-goitre area without iodine prophylaxis. Electron microscopic findings confirm the histological diagnosis and outline the oncocytic cell against light-microscopically similar cells.", "contents": "[Familial occurrence of onocytic carcinoma of the thyroid gland during pregnancy (author's transl)]. Two cases of oncocytic papillary carcinoma of the thyroid gland are reported, which occurred in two sisters during pregnancy. In both cases the follow-up is without complications (after strumectomy and postoperative radiation) and therefore it is in strong contrast to the aggressive biological behaviour during pregnancy. The oncocytic papillary carcinoma is a rare disease in the endemic-goitre area without iodine prophylaxis. Electron microscopic findings confirm the histological diagnosis and outline the oncocytic cell against light-microscopically similar cells."}
{"id": "PMID:463392", "title": "[Indication for treatment of gastric and duodenal ulcers by resection (BI/II) (author's transl)].", "content": "After exposing the indications for resections according to Billroth, the results achieved are analysed, advantages and disadvantages of the method are discussed with regard to late results. A strict indication lowers the motality rate, postprandial disorders, anastomotic ulceration and carcinoma of the gastric stump. New operative methods like vagotomy and its modifications should complete the surgical armamentarium, but cannot replace the classic gastric resection.", "contents": "[Indication for treatment of gastric and duodenal ulcers by resection (BI/II) (author's transl)]. After exposing the indications for resections according to Billroth, the results achieved are analysed, advantages and disadvantages of the method are discussed with regard to late results. A strict indication lowers the motality rate, postprandial disorders, anastomotic ulceration and carcinoma of the gastric stump. New operative methods like vagotomy and its modifications should complete the surgical armamentarium, but cannot replace the classic gastric resection."}
{"id": "PMID:463393", "title": "[Topography of benign nonepithelial tumours of the stomach (author's transl)].", "content": "In a series of 500 unselected autopsy cases 23 myomas, 14 neurinomas and 5 lipomas of the stomach were met. Benign tumours are mainly localized in the corpus and fundus of the stomach.", "contents": "[Topography of benign nonepithelial tumours of the stomach (author's transl)]. In a series of 500 unselected autopsy cases 23 myomas, 14 neurinomas and 5 lipomas of the stomach were met. Benign tumours are mainly localized in the corpus and fundus of the stomach."}
{"id": "PMID:463394", "title": "[Chronic pancreatitis; surgical therapy (author's transl)].", "content": "41 patients suffering from chronic pancreatitis underwent surgery from 1965 to May 1978. Mortality rate was 4,8%. Drainage operations and resections are the methods of choice today.", "contents": "[Chronic pancreatitis; surgical therapy (author's transl)]. 41 patients suffering from chronic pancreatitis underwent surgery from 1965 to May 1978. Mortality rate was 4,8%. Drainage operations and resections are the methods of choice today."}
{"id": "PMID:463395", "title": "[Surgery in combined aorto-iliac and femoro-popliteal occlusive lesions (author's transl)].", "content": "In combined chronic occlusive lesions at the aorto-iliac and femoro-popliteal level the partial repair of the central vessel in combination with profundaplasty and lumbar sympathectomy (\"triad procedure\") represents a simpler and shorter procedure than the previously preferred total correction of both segments. In a retrospective study of 403 limbs (stage II-IV) the triad procedure shows a cumulative patency (life table analysis) of 77% vs. 39% in total repair. The amputation rate in triad procedure--for stage III/IV--was 8% vs. 36% and the frequency of reoperation 7% vs. 23% in total repair. Prerequisites for this type of repair are an optimal technique and a correct selection of patients presenting patient \"receiving segment\" of the popliteal artery and a sufficient run-off below the knee.", "contents": "[Surgery in combined aorto-iliac and femoro-popliteal occlusive lesions (author's transl)]. In combined chronic occlusive lesions at the aorto-iliac and femoro-popliteal level the partial repair of the central vessel in combination with profundaplasty and lumbar sympathectomy (\"triad procedure\") represents a simpler and shorter procedure than the previously preferred total correction of both segments. In a retrospective study of 403 limbs (stage II-IV) the triad procedure shows a cumulative patency (life table analysis) of 77% vs. 39% in total repair. The amputation rate in triad procedure--for stage III/IV--was 8% vs. 36% and the frequency of reoperation 7% vs. 23% in total repair. Prerequisites for this type of repair are an optimal technique and a correct selection of patients presenting patient \"receiving segment\" of the popliteal artery and a sufficient run-off below the knee."}
{"id": "PMID:463396", "title": "[Operative treatment of deep vein thrombosis (author's transl)].", "content": "From 1973 to 1977 a total of 63 patients with thrombosis of the deep veins in leg and pelvis and in the V. cava inferior was treated by operative thrombectomy. Prior to the operation all patients were submitted to phlebography. The mean age of all was 52 years. One patient died from pulmonary embolism during thrombectomy, there was no further hospital mortality. Because of low mortality incidence and rarely postthrombotic complications we prefer the operative treatment of deep vein thrombosis. The preoperative cavography helps to avoid pulmonary embolism during thrombectomy.", "contents": "[Operative treatment of deep vein thrombosis (author's transl)]. From 1973 to 1977 a total of 63 patients with thrombosis of the deep veins in leg and pelvis and in the V. cava inferior was treated by operative thrombectomy. Prior to the operation all patients were submitted to phlebography. The mean age of all was 52 years. One patient died from pulmonary embolism during thrombectomy, there was no further hospital mortality. Because of low mortality incidence and rarely postthrombotic complications we prefer the operative treatment of deep vein thrombosis. The preoperative cavography helps to avoid pulmonary embolism during thrombectomy."}
{"id": "PMID:463397", "title": "[Comparative histological and histochemical studies on the \"spleen-ovary\" in the rat (Lipsch\u00fctz) following administration of FSH and spleen extract].", "content": "On the basis of the \"Lipschuetz-ovary\" model the rat ovary is cystically altered. -- In such an ovary the effect of a spleen extract (Solcosplen) and FSH (Anternon) were compared with histological and histochemical methods. -- With bothe the substances could be observed a diminution of the cystic degeneration.", "contents": "[Comparative histological and histochemical studies on the \"spleen-ovary\" in the rat (Lipsch\u00fctz) following administration of FSH and spleen extract]. On the basis of the \"Lipschuetz-ovary\" model the rat ovary is cystically altered. -- In such an ovary the effect of a spleen extract (Solcosplen) and FSH (Anternon) were compared with histological and histochemical methods. -- With bothe the substances could be observed a diminution of the cystic degeneration."}
{"id": "PMID:463398", "title": "[Examination of hormonal contraceptives by enzyme induction (author's transl)].", "content": "The influence of drug stimulated biotransformation on the biological effectiveness of two different contraceptives (Deposition and Gravistat) was observed.--Phenobarbital, a potent enzyme inducer, is known to increase the metabolism of steroid hormones, including estrogens and progrestogens. -- The clinical symptoms of drug mediated enzyme induction of hormonal steroid contraceptives are dysfunctional bleedings. This phenomenon is discussed.", "contents": "[Examination of hormonal contraceptives by enzyme induction (author's transl)]. The influence of drug stimulated biotransformation on the biological effectiveness of two different contraceptives (Deposition and Gravistat) was observed.--Phenobarbital, a potent enzyme inducer, is known to increase the metabolism of steroid hormones, including estrogens and progrestogens. -- The clinical symptoms of drug mediated enzyme induction of hormonal steroid contraceptives are dysfunctional bleedings. This phenomenon is discussed."}
{"id": "PMID:463399", "title": "[Investigation of hydroxyproline by hormonal contraception (author's transl)].", "content": "The effect of hormonal contraceptives administered for 24 months on the hydroxyproline content in urine was investigated. -- The results suggest an increase of hydroxyproline by application of sexual steroids. The reversibility is discussed.", "contents": "[Investigation of hydroxyproline by hormonal contraception (author's transl)]. The effect of hormonal contraceptives administered for 24 months on the hydroxyproline content in urine was investigated. -- The results suggest an increase of hydroxyproline by application of sexual steroids. The reversibility is discussed."}
{"id": "PMID:463400", "title": "[A prospective trial on osteoporosis following bilateral ovariectomy with and without oestrogen replacement (author's transl)].", "content": "During four years a prospective study was carried out on 59 women, who had ovariectomized bilaterally for non-malignant diseases. It was studied the loss of the body length, the loss of bone mineral content in a middle phalanx expressed as percentage and several x-ray criteria. By means of combined performance signs of a beginning osteoporosis were found in 59 per cent of women after 2 years and in 68 per cent after 4 years after ovariectomy. -- The authors recommend relative simple screening methods: observation of body length, the dual beam x-ray spectrophotometry (Krokowski) and the minimal combined cortex thickness of the proximal radius according Meema. Women undergoing ovariectomy before the menopause should be attended by medical teamwork. The results of oestrogen prophylaxis were not so convincing as other investigators described.", "contents": "[A prospective trial on osteoporosis following bilateral ovariectomy with and without oestrogen replacement (author's transl)]. During four years a prospective study was carried out on 59 women, who had ovariectomized bilaterally for non-malignant diseases. It was studied the loss of the body length, the loss of bone mineral content in a middle phalanx expressed as percentage and several x-ray criteria. By means of combined performance signs of a beginning osteoporosis were found in 59 per cent of women after 2 years and in 68 per cent after 4 years after ovariectomy. -- The authors recommend relative simple screening methods: observation of body length, the dual beam x-ray spectrophotometry (Krokowski) and the minimal combined cortex thickness of the proximal radius according Meema. Women undergoing ovariectomy before the menopause should be attended by medical teamwork. The results of oestrogen prophylaxis were not so convincing as other investigators described."}
{"id": "PMID:463401", "title": "[Experiences using the suction curettage system of Hamann and Pockrand (author's transl)].", "content": "All curettages of a 8-week-period were done using the suction system of Hamann and Pockrandt. This test-collective includes 66 patients, their age was between 20 and 78 years; 6 of them had to be excluded: the surgical intervention was begun without general anaesthesia but continued with general anaesthesia is required by the patient. The test-collective was divided into 2 groups: in group A (30 patients) all suction-curettages were done in general anaesthesia, in the first 20 patients a conventional curettage with dilation of the cervix were performed additionally, in order to proof the effectiveness of the suction curettage. The histological findings of the materials of the conventional curettages had shown no more informations than that of the materials of the suction curettages. -- In group B (30 patients) the suction curettage were done in diazepampentazocin-analgesia; a third of them had specified no  sensations, the others had stated a feeling of pressure or spasms. -- In each group the histological examinations were possible without any problems. The advantages of the suction curettage system are the rare necessity of cervix dilatation (7 of 60 cases), the possibility of the operation without general anaesthesia and the possibility of a quick performance of the curettage.", "contents": "[Experiences using the suction curettage system of Hamann and Pockrand (author's transl)]. All curettages of a 8-week-period were done using the suction system of Hamann and Pockrandt. This test-collective includes 66 patients, their age was between 20 and 78 years; 6 of them had to be excluded: the surgical intervention was begun without general anaesthesia but continued with general anaesthesia is required by the patient. The test-collective was divided into 2 groups: in group A (30 patients) all suction-curettages were done in general anaesthesia, in the first 20 patients a conventional curettage with dilation of the cervix were performed additionally, in order to proof the effectiveness of the suction curettage. The histological findings of the materials of the conventional curettages had shown no more informations than that of the materials of the suction curettages. -- In group B (30 patients) the suction curettage were done in diazepampentazocin-analgesia; a third of them had specified no  sensations, the others had stated a feeling of pressure or spasms. -- In each group the histological examinations were possible without any problems. The advantages of the suction curettage system are the rare necessity of cervix dilatation (7 of 60 cases), the possibility of the operation without general anaesthesia and the possibility of a quick performance of the curettage."}
{"id": "PMID:463402", "title": "[Extended prevention of uterine cervix cancer].", "content": "A transverse-oval os uteri or Emmet' tear are the result of a tear at the mouth of the womb during final cervical dilatation. Furthermore, they are the cause of a more or less developed ectopia and combined cervical and vaginal fluor with concomitant colpitis. These alterations also represent the foundation not the cause, of a development of cervical carcinoma. In the sense of prophylactic reasoning the elimination of the symptoms mentioned above should be considered. -- The use of the speculum and care of the suture of the nearly always torn os uteri after each delivery must be the first step to avoid the development of a collum carcinoma. -- In the second place, electrocoagulation or shallow conisation with subsequent coagulation and cryo-surgical treatment of the ectopia has to be carried out. -- Therefore the obstetrician decides whether the patient -- in view of developing a collum carcinoma -- becomes a risk patient. He has to ensure that an almost normal portio uteri returns without ectropion and Emmet'tear and also without cervical and vaginal fluor, which could trouble the patient considerably. -- After every delivery, therefore, the speculum should be used. At the same time a cervix tear haemorrhage, one of the most frequent exsanguinations, can be avoided.", "contents": "[Extended prevention of uterine cervix cancer]. A transverse-oval os uteri or Emmet' tear are the result of a tear at the mouth of the womb during final cervical dilatation. Furthermore, they are the cause of a more or less developed ectopia and combined cervical and vaginal fluor with concomitant colpitis. These alterations also represent the foundation not the cause, of a development of cervical carcinoma. In the sense of prophylactic reasoning the elimination of the symptoms mentioned above should be considered. -- The use of the speculum and care of the suture of the nearly always torn os uteri after each delivery must be the first step to avoid the development of a collum carcinoma. -- In the second place, electrocoagulation or shallow conisation with subsequent coagulation and cryo-surgical treatment of the ectopia has to be carried out. -- Therefore the obstetrician decides whether the patient -- in view of developing a collum carcinoma -- becomes a risk patient. He has to ensure that an almost normal portio uteri returns without ectropion and Emmet'tear and also without cervical and vaginal fluor, which could trouble the patient considerably. -- After every delivery, therefore, the speculum should be used. At the same time a cervix tear haemorrhage, one of the most frequent exsanguinations, can be avoided."}
{"id": "PMID:463403", "title": "[Various healing results in adenocarcinoma and squamous-cell carcinoma of the cervix uteri].", "content": "An account is given on the difference in prognosis between squamous cell carcinoma and adenocarcinoma of the cervix. -- The 5-year-healing reaches by adenocarcinoma up to 65.3%, by epithelial carcinoma up to 50.1%. -- Possible causes of carcinoma are referred to. Conclusions are pointed out.", "contents": "[Various healing results in adenocarcinoma and squamous-cell carcinoma of the cervix uteri]. An account is given on the difference in prognosis between squamous cell carcinoma and adenocarcinoma of the cervix. -- The 5-year-healing reaches by adenocarcinoma up to 65.3%, by epithelial carcinoma up to 50.1%. -- Possible causes of carcinoma are referred to. Conclusions are pointed out."}
{"id": "PMID:463404", "title": "[Lymphography in malignant vulvar and vaginal tumors. 1. Clinical significance].", "content": "The results of a retrograde analysis of lymphographic processes in 111 patients with malign vulvar and vaginal tumours are substantiating statements concerning the importance of lymphography in diagnosing metastastic processes in lymph-nodes of the inguinal and retroperitoneal region. -- Metastases could be proved lymphographically in 24 (34%) out of 70 carcinomas of the vulva and 8 (22%) out of 37 carcinomas of the vagina. In 24% of all patients with carcinomas of the vulva there were lymph-nodes involved, in two of these cases without a simultaneous involvement of the inguinal region. With carcinomas of the vagina a lumbar involvement was found in 50% of the cases. The accuracy of lymphographic diagnosis was 79% regarding the inguinal region and 89% in the iliac region. Despite of the lower accuracy with regard to inguinal lymph-nodes lymphography exceeds findings obtained by clinical palpation in this respect. As a result of the study, the use of lymphography in case of vulvar and vaginal carcinomas of all clinical stages is considered to be profitable for diagnosing and therapy planning.", "contents": "[Lymphography in malignant vulvar and vaginal tumors. 1. Clinical significance]. The results of a retrograde analysis of lymphographic processes in 111 patients with malign vulvar and vaginal tumours are substantiating statements concerning the importance of lymphography in diagnosing metastastic processes in lymph-nodes of the inguinal and retroperitoneal region. -- Metastases could be proved lymphographically in 24 (34%) out of 70 carcinomas of the vulva and 8 (22%) out of 37 carcinomas of the vagina. In 24% of all patients with carcinomas of the vulva there were lymph-nodes involved, in two of these cases without a simultaneous involvement of the inguinal region. With carcinomas of the vagina a lumbar involvement was found in 50% of the cases. The accuracy of lymphographic diagnosis was 79% regarding the inguinal region and 89% in the iliac region. Despite of the lower accuracy with regard to inguinal lymph-nodes lymphography exceeds findings obtained by clinical palpation in this respect. As a result of the study, the use of lymphography in case of vulvar and vaginal carcinomas of all clinical stages is considered to be profitable for diagnosing and therapy planning."}
{"id": "PMID:463405", "title": "[Rare cause of death in pulmonary recurrence of uterine cancer].", "content": "A brief report on endometrium carcinoma with lung metastases is given. The obturation of the main bronchus and trachea by lymphonodular metastases is a rare cause of death.", "contents": "[Rare cause of death in pulmonary recurrence of uterine cancer]. A brief report on endometrium carcinoma with lung metastases is given. The obturation of the main bronchus and trachea by lymphonodular metastases is a rare cause of death."}
{"id": "PMID:463406", "title": "[Knowledge of uterine lipomas].", "content": "Report of a rare case of an uterine lipoma. Review of the literature with regard to frequency, site, size, age and clinical symptoms. Differential diagnosis and pathogenesis have been discussed.", "contents": "[Knowledge of uterine lipomas]. Report of a rare case of an uterine lipoma. Review of the literature with regard to frequency, site, size, age and clinical symptoms. Differential diagnosis and pathogenesis have been discussed."}
{"id": "PMID:463407", "title": "[The microprobe excision as a search biopsy within the scope of cancer-trace detection on the cervix uteri].", "content": "Besides colposcopy and cytology microbiopsy as a detection method for early detection of malign cervical lesions was applied to 142 women patients of the UFK Halle. A precondition for the successfull application of this method is a carefull preliminary colposcopic examination and close co-operation with the histologist. The interim result of the microbiopsy corresponded to a high degree with the definitive result. In this way, it was possible to indicate 20% of the preclinical carcinoma for histological evidence despite of negative cytology. An individual combination of all 3 ways of research guarantee a maximum of certainty for the early finding of cervical carcinoma and their pre-stages.", "contents": "[The microprobe excision as a search biopsy within the scope of cancer-trace detection on the cervix uteri]. Besides colposcopy and cytology microbiopsy as a detection method for early detection of malign cervical lesions was applied to 142 women patients of the UFK Halle. A precondition for the successfull application of this method is a carefull preliminary colposcopic examination and close co-operation with the histologist. The interim result of the microbiopsy corresponded to a high degree with the definitive result. In this way, it was possible to indicate 20% of the preclinical carcinoma for histological evidence despite of negative cytology. An individual combination of all 3 ways of research guarantee a maximum of certainty for the early finding of cervical carcinoma and their pre-stages."}
{"id": "PMID:463408", "title": "[On the therapy of uterine cervix carcinoma in situ].", "content": "It is reported of 607 cases of uterine cervix carcinoma in situ from the years 1963 to 1977. During this period, the diagnostic and therapeutic procedures as well as the histological preparation technique have advanced. -- 75% of the cases were primarily conized. About half of these cases were surely radically excised. 323 patients (53,2%) were treated actively by simple hysterectomy, 242 patients (46,8%) were treated conservatively (190 conisations, 19 reconisations, 60 portioamputations, 12 ringbiopsies, 3 radiations). -- After 323 total exstirpations, 2 recurrences of carcinoma in situ in vaginal stump were observed (0,6%). After local treatment of 242 patients, 17 recurrences of the disease appeared (6%), among them 4 cases as invasive cervix carcinoma. 11 cases belong to the group of primarily nonexcised or uncertainly radically excised carcinoma in situ, and in 4 cases a possibility of recurrence has been discussed. -- It has been refered to the importance of the exact conisation technique and subtile histotechnical obtaining of slides in step sections. If the carcinoma in situ radically excised and followed by an attentive follow up in women below 40 years, a conservative procedure is adviced. For women above 40 years an active procedure is indicated, because it offers a high therapeutic reliability and a minimal risk. For cases of nonradically excised carcinoma in situ hysterectomy is indicated. The removal of the vaginal cuff is considered as unnecessary.", "contents": "[On the therapy of uterine cervix carcinoma in situ]. It is reported of 607 cases of uterine cervix carcinoma in situ from the years 1963 to 1977. During this period, the diagnostic and therapeutic procedures as well as the histological preparation technique have advanced. -- 75% of the cases were primarily conized. About half of these cases were surely radically excised. 323 patients (53,2%) were treated actively by simple hysterectomy, 242 patients (46,8%) were treated conservatively (190 conisations, 19 reconisations, 60 portioamputations, 12 ringbiopsies, 3 radiations). -- After 323 total exstirpations, 2 recurrences of carcinoma in situ in vaginal stump were observed (0,6%). After local treatment of 242 patients, 17 recurrences of the disease appeared (6%), among them 4 cases as invasive cervix carcinoma. 11 cases belong to the group of primarily nonexcised or uncertainly radically excised carcinoma in situ, and in 4 cases a possibility of recurrence has been discussed. -- It has been refered to the importance of the exact conisation technique and subtile histotechnical obtaining of slides in step sections. If the carcinoma in situ radically excised and followed by an attentive follow up in women below 40 years, a conservative procedure is adviced. For women above 40 years an active procedure is indicated, because it offers a high therapeutic reliability and a minimal risk. For cases of nonradically excised carcinoma in situ hysterectomy is indicated. The removal of the vaginal cuff is considered as unnecessary."}
{"id": "PMID:463409", "title": "[Effectiveness of adjuvant chemotherapy in ovarian carcinoma].", "content": "A report is given about the efficiency of the so called adjuvant chemotherapy in 26 cases (26 patients suffering from ovarian cancer in the stage T1M0N0) in a time of 10 years (1968-1977).--After the primary operation-therapy, a part of the patients (9) got the cytostatic drugs Trenimon or Cyclophosphamid. The patients of the control group got nothing. The survival times of both groups showed no significant distinctions. -- The results show that the adjuvant chemotherapy in cases of ovarian cancers in stage T1M0N0 can not be recommended because there are too many side effects of the these cytostatic drugs.", "contents": "[Effectiveness of adjuvant chemotherapy in ovarian carcinoma]. A report is given about the efficiency of the so called adjuvant chemotherapy in 26 cases (26 patients suffering from ovarian cancer in the stage T1M0N0) in a time of 10 years (1968-1977).--After the primary operation-therapy, a part of the patients (9) got the cytostatic drugs Trenimon or Cyclophosphamid. The patients of the control group got nothing. The survival times of both groups showed no significant distinctions. -- The results show that the adjuvant chemotherapy in cases of ovarian cancers in stage T1M0N0 can not be recommended because there are too many side effects of the these cytostatic drugs."}
{"id": "PMID:463410", "title": "[Glucose assimilation under high-dose norethisterone-acetate therapy in adenocarcinoma of the corpus uteri].", "content": "In 29 patients suffering from adenocarcinom of the corpus uteri, who had been treated with high doses of norethisterone acetate (NEA) alone or in combination with irradiation, we performed a study in order to find out whether NEA will produce a clinically measurable and noteworthy modification of glucose tolerance. -- For this purpose, the glucose assimilation coefficient (k value), apart from its first determination, will be determined 6 weeks later under current therapy. The comparison of both values shows a statistically significant decrease of the k value after 6 weeks in all patients whose first value was k less than or equal to 1. For the patients with a first value of k greater than 1 a significant development was not detectable. The alteration of the k value under NEA therapy in patients with a primarily decreased value (n = 11; in 5 of them diabetes known) was accompanied merely by 2 diabetics with changes in the day profile of such an extent that a correction of therapy was necessary.", "contents": "[Glucose assimilation under high-dose norethisterone-acetate therapy in adenocarcinoma of the corpus uteri]. In 29 patients suffering from adenocarcinom of the corpus uteri, who had been treated with high doses of norethisterone acetate (NEA) alone or in combination with irradiation, we performed a study in order to find out whether NEA will produce a clinically measurable and noteworthy modification of glucose tolerance. -- For this purpose, the glucose assimilation coefficient (k value), apart from its first determination, will be determined 6 weeks later under current therapy. The comparison of both values shows a statistically significant decrease of the k value after 6 weeks in all patients whose first value was k less than or equal to 1. For the patients with a first value of k greater than 1 a significant development was not detectable. The alteration of the k value under NEA therapy in patients with a primarily decreased value (n = 11; in 5 of them diabetes known) was accompanied merely by 2 diabetics with changes in the day profile of such an extent that a correction of therapy was necessary."}
{"id": "PMID:463411", "title": "[Clinical experience with the copper T-200 intrauterine device].", "content": "It is reported on experiences with 138 Cooper-T-200 IUDs, resulting in 1868 months of use. Both the Pearl-Index with 1,3 and the expulsion rate with 1,44% are no significant lower, than in a compared DANA-Super-IUD collective. In both groups the rate of all events like pains, spottings and hypermenorrhea is almost identically. Hypermeorrhea is of significant frequenter occur in the Copper-T-IUDs.", "contents": "[Clinical experience with the copper T-200 intrauterine device]. It is reported on experiences with 138 Cooper-T-200 IUDs, resulting in 1868 months of use. Both the Pearl-Index with 1,3 and the expulsion rate with 1,44% are no significant lower, than in a compared DANA-Super-IUD collective. In both groups the rate of all events like pains, spottings and hypermenorrhea is almost identically. Hypermeorrhea is of significant frequenter occur in the Copper-T-IUDs."}
{"id": "PMID:463413", "title": "[Gynecologic geriatric surgery at the University Gynecological Clinic Rostock during 1960--1974].", "content": "There is reported about 2279 gynaecological operations at women, aged 60 years and more, in a period from 1960 to 1974, divided into three 5-years-groups. The part of geriatric operations increased from 5,3% to 7,0% of all operations. The division into high risk-groups took place after points given by Loskant. 41,1% of all patients where classified into group III. Group IV has the highest postoperative mortality rate (5,3%). The postoperative mortality rate altogether was 2,2%. In the years from 1960 to 1974 it decreased from 2,6% to 1,9%. 25,1% of the surgical operations where carried out abdominaly. The high part of vaginal operations comes off by placing curettages into the analysis. Under that aspect must be seen the postoperative mortality rate 7,1% at abdominal and 0,7% at vaginal operations. In the main point indications for operation were malignant tumours (37,3%), haemorrhages (25,4%), benign tumours (17,6%), and position variations of the genitals (10,6%).", "contents": "[Gynecologic geriatric surgery at the University Gynecological Clinic Rostock during 1960--1974]. There is reported about 2279 gynaecological operations at women, aged 60 years and more, in a period from 1960 to 1974, divided into three 5-years-groups. The part of geriatric operations increased from 5,3% to 7,0% of all operations. The division into high risk-groups took place after points given by Loskant. 41,1% of all patients where classified into group III. Group IV has the highest postoperative mortality rate (5,3%). The postoperative mortality rate altogether was 2,2%. In the years from 1960 to 1974 it decreased from 2,6% to 1,9%. 25,1% of the surgical operations where carried out abdominaly. The high part of vaginal operations comes off by placing curettages into the analysis. Under that aspect must be seen the postoperative mortality rate 7,1% at abdominal and 0,7% at vaginal operations. In the main point indications for operation were malignant tumours (37,3%), haemorrhages (25,4%), benign tumours (17,6%), and position variations of the genitals (10,6%)."}
{"id": "PMID:463415", "title": "[The reverse effect of sexualsteroids on the serum-lysozyme (author's transl)].", "content": "75 women received different preparates of contraceptives (Non-Ovlon, Ovosiston, Gravistat, Deposistion--VEB Jenapharm Jena, GDR) for two years. In the last month of the application of the sexual steroids and one month later the lysozyme titer in the sera was tested to determine a reverse or stop-effect. An elevation of the muramidase could be shown in all cases. This findings are interpreted in relation to a non manifest influence of sexualsteroids on the lysozyme.", "contents": "[The reverse effect of sexualsteroids on the serum-lysozyme (author's transl)]. 75 women received different preparates of contraceptives (Non-Ovlon, Ovosiston, Gravistat, Deposistion--VEB Jenapharm Jena, GDR) for two years. In the last month of the application of the sexual steroids and one month later the lysozyme titer in the sera was tested to determine a reverse or stop-effect. An elevation of the muramidase could be shown in all cases. This findings are interpreted in relation to a non manifest influence of sexualsteroids on the lysozyme."}
{"id": "PMID:463416", "title": "[Radioimmunological LH and FSH determination from urine collected within 24 hours].", "content": "Sensitive radioimmunoassays for measurement of urinary human LH and FSH by using either pituitary or urinary LH and FSH standard preparations are described. In healthy subjects mean LH and FSH values in 24-h-urine specimens were determined which are similar to those reported by others: LH: 22.6 IU/24-h (males), 17.8 IU/24-h (females), 49.1 IU/24-h (postmenopausal females); FSH: 6.0 IU/24-h (males), 15.0 IU/24 h (females), 48.5 IU/24-h (postmenopausal females). Because of the high sensitivity and practibility we believe that these radioimmunoassays are clinically useful approaches in assessment of pituitary and gonadal disorders.", "contents": "[Radioimmunological LH and FSH determination from urine collected within 24 hours]. Sensitive radioimmunoassays for measurement of urinary human LH and FSH by using either pituitary or urinary LH and FSH standard preparations are described. In healthy subjects mean LH and FSH values in 24-h-urine specimens were determined which are similar to those reported by others: LH: 22.6 IU/24-h (males), 17.8 IU/24-h (females), 49.1 IU/24-h (postmenopausal females); FSH: 6.0 IU/24-h (males), 15.0 IU/24 h (females), 48.5 IU/24-h (postmenopausal females). Because of the high sensitivity and practibility we believe that these radioimmunoassays are clinically useful approaches in assessment of pituitary and gonadal disorders."}
{"id": "PMID:463417", "title": "[Cell-mediated immunity in myoma uteri measured by the leukocyte migration inhibition test (author's transl)].", "content": "The leukocyte migration inhibition test was performed on 56 patients hysterectomized on account of myoma. -- Despite low antigenicity of autologous myoma tissue significant differences in inhibitions of migration were observed depending on the extent of surgical radicality in the adnexal region. -- Non-inhibition of leukocyte migration in patients whose myomatous uterus alone was exstirpated is explained by the oestrogens produced in the remaining ovaries.", "contents": "[Cell-mediated immunity in myoma uteri measured by the leukocyte migration inhibition test (author's transl)]. The leukocyte migration inhibition test was performed on 56 patients hysterectomized on account of myoma. -- Despite low antigenicity of autologous myoma tissue significant differences in inhibitions of migration were observed depending on the extent of surgical radicality in the adnexal region. -- Non-inhibition of leukocyte migration in patients whose myomatous uterus alone was exstirpated is explained by the oestrogens produced in the remaining ovaries."}
{"id": "PMID:463418", "title": "[Clinical value of various parameters in antenatal diagnosis of fetal hypotrophia (intrauterine retardation) (author's transl)].", "content": "Retrospective evaluation of percental frequency of pathological data gained by prepartual monitoring in pregnancies at risk of 180 small for gestational age infants and 130 eutrophic ones at term (postnatal classification). Parameters of interest were continuous maternal gain of weight, monthly progress of the size of the uterus, continuous ultrasomic cephalometria, maternal urinary estriol and serum heat stable alkaline phosphatase as well as amnioscopia and cardiotokographia with and without oxytocin challenge test. -- It seems that there is a significant better prediction of fetal retardation with the help of common clinical methods as well as with ultrasonic and that they are superior to the examined biochemical, cardiotocographical and amnioscopical parameters. With regard to possible additional disturbances of the fetoplacental unit--especially of respiratory placental function--in suspected intrauterine retardation the complete monitoring program should be taken.", "contents": "[Clinical value of various parameters in antenatal diagnosis of fetal hypotrophia (intrauterine retardation) (author's transl)]. Retrospective evaluation of percental frequency of pathological data gained by prepartual monitoring in pregnancies at risk of 180 small for gestational age infants and 130 eutrophic ones at term (postnatal classification). Parameters of interest were continuous maternal gain of weight, monthly progress of the size of the uterus, continuous ultrasomic cephalometria, maternal urinary estriol and serum heat stable alkaline phosphatase as well as amnioscopia and cardiotokographia with and without oxytocin challenge test. -- It seems that there is a significant better prediction of fetal retardation with the help of common clinical methods as well as with ultrasonic and that they are superior to the examined biochemical, cardiotocographical and amnioscopical parameters. With regard to possible additional disturbances of the fetoplacental unit--especially of respiratory placental function--in suspected intrauterine retardation the complete monitoring program should be taken."}
{"id": "PMID:463419", "title": "[Mode of delivery, postnatal condition and neonatal outcome in preterm infants].", "content": "There were under examination 578 preterm infants of two groups--28. up to 31. and 32. up to 36. week of gestation--after birth of vertex as well as breech prevention. Postnatal condition and neonatal outcome were put into relation to the mode of delivery. We compared Apgar-Score (one and five minute value), morbidity on respiratory distress syndrom as well as rate of survival and neonatal mortality in spontaneous delivery with and without episiotomia, with specula delivery, Shute-forceps and vacuum extraction of vertex presentation as well as with breech presentation after vaginal delivery and primary Caesarean section. In respect of the management of the second stage of labour it is our opinion that prophylactic additional measures in preterm delivery of vertex presentation after 32 weeks of gestation are not necessary and that this question should be examined in a larger study of much more cases. But we were able to demonstrate that up to 32. week of gestation well-timed episiotomia of optimal size is necessary. Our good experiences in breech presentation between 31. and 35. week of gestation treated by obligate Caesarean section have to prove true in future.", "contents": "[Mode of delivery, postnatal condition and neonatal outcome in preterm infants]. There were under examination 578 preterm infants of two groups--28. up to 31. and 32. up to 36. week of gestation--after birth of vertex as well as breech prevention. Postnatal condition and neonatal outcome were put into relation to the mode of delivery. We compared Apgar-Score (one and five minute value), morbidity on respiratory distress syndrom as well as rate of survival and neonatal mortality in spontaneous delivery with and without episiotomia, with specula delivery, Shute-forceps and vacuum extraction of vertex presentation as well as with breech presentation after vaginal delivery and primary Caesarean section. In respect of the management of the second stage of labour it is our opinion that prophylactic additional measures in preterm delivery of vertex presentation after 32 weeks of gestation are not necessary and that this question should be examined in a larger study of much more cases. But we were able to demonstrate that up to 32. week of gestation well-timed episiotomia of optimal size is necessary. Our good experiences in breech presentation between 31. and 35. week of gestation treated by obligate Caesarean section have to prove true in future."}
{"id": "PMID:463420", "title": "[Desoxycytydil-deaminase activity in obstetrics].", "content": "The Deoxycytidyl-Deaminase activity in the plasma of 75 woman with a normal or complicated pregnancy was estimated. The control group consisted of 20 healthy, non-pregnant woman. Only in the cases of pregnancies with trisymptomic toxaemia and chronic kidney diseases high enzyme activities were estimated. There are no timedependent changes in the enzym activity during a normal pregnancy. The estimation of the enzyme activity of DCMP-Deaminase in the plasma seems to be a valuable method for the registration of pregnant woman with classical EPH-Gestosis and chronic kidney diseases.", "contents": "[Desoxycytydil-deaminase activity in obstetrics]. The Deoxycytidyl-Deaminase activity in the plasma of 75 woman with a normal or complicated pregnancy was estimated. The control group consisted of 20 healthy, non-pregnant woman. Only in the cases of pregnancies with trisymptomic toxaemia and chronic kidney diseases high enzyme activities were estimated. There are no timedependent changes in the enzym activity during a normal pregnancy. The estimation of the enzyme activity of DCMP-Deaminase in the plasma seems to be a valuable method for the registration of pregnant woman with classical EPH-Gestosis and chronic kidney diseases."}
{"id": "PMID:463421", "title": "[The conduction of labor and postlabor periods following cesarean section].", "content": "Out of 11315 childbirths under review, 109 women (0.96%) with state after secio caesarea were delivered of a child in the period from 1970 to 1978. In 63.3% of the cases it was possible to achieve a vaginal birth. With 450 caesarean sections, 2 cases of the mother mortality occurred. -- A comparison between the sectio and the re-sectio indications does not show any difference. -- Uterus rupture was noticed in 2 cases. -- In a comparative study, general palpating of the uterus is contrasted with a conservative behaviour involving haemorrhage, blood-pressure, and pulse controls. -- As a result, manual re-palpating is again recommended, however, should not be forced. In certain individual cases a conservative behaviour is also justified.", "contents": "[The conduction of labor and postlabor periods following cesarean section]. Out of 11315 childbirths under review, 109 women (0.96%) with state after secio caesarea were delivered of a child in the period from 1970 to 1978. In 63.3% of the cases it was possible to achieve a vaginal birth. With 450 caesarean sections, 2 cases of the mother mortality occurred. -- A comparison between the sectio and the re-sectio indications does not show any difference. -- Uterus rupture was noticed in 2 cases. -- In a comparative study, general palpating of the uterus is contrasted with a conservative behaviour involving haemorrhage, blood-pressure, and pulse controls. -- As a result, manual re-palpating is again recommended, however, should not be forced. In certain individual cases a conservative behaviour is also justified."}
{"id": "PMID:463422", "title": "[Reinfection in brucellosis].", "content": "The results of clinical observation on 68 recurrent cases of brucellosis, occurring a long time (3 to 30 years) after the primary disease, are presented. As the recurrent disease developed against an unfavorable premorbid background, a considerable severity in the clinical manifestations of brucellosis was observed.", "contents": "[Reinfection in brucellosis]. The results of clinical observation on 68 recurrent cases of brucellosis, occurring a long time (3 to 30 years) after the primary disease, are presented. As the recurrent disease developed against an unfavorable premorbid background, a considerable severity in the clinical manifestations of brucellosis was observed."}
{"id": "PMID:463423", "title": "[Characteristics of the precipitation reaction at the stage of antigen-antibody complex aggregate formation studied by a spectroturbidimetric method].", "content": "The possibility of using the spectroturbidimetric method, one of the variants of the light scattering method, for the determination of the characteristics of the second phase of the antigen-antibody reaction was shown examplified by the interaction between rabbit antiserum and bovine serum albumin. The parameters of the aggregates formed in the process (their average size, their concentration by weight and number) were calculated for the whole period of the reaction and with its components taken in different proportions. The precipitation curve plotted by the above method correlated well with the result of an independent determination at an extinction coefficient of E278. A conclusion was drawn, based on special evaluation, that hydrophobic interactions have a predominant influence on the formation of antigen-antibody aggregates. A quantitative approach was proposed for studying the mechanism of the antigen-antibody aggregate formation and for the analytical determination of various classes of immunoglobulins.", "contents": "[Characteristics of the precipitation reaction at the stage of antigen-antibody complex aggregate formation studied by a spectroturbidimetric method]. The possibility of using the spectroturbidimetric method, one of the variants of the light scattering method, for the determination of the characteristics of the second phase of the antigen-antibody reaction was shown examplified by the interaction between rabbit antiserum and bovine serum albumin. The parameters of the aggregates formed in the process (their average size, their concentration by weight and number) were calculated for the whole period of the reaction and with its components taken in different proportions. The precipitation curve plotted by the above method correlated well with the result of an independent determination at an extinction coefficient of E278. A conclusion was drawn, based on special evaluation, that hydrophobic interactions have a predominant influence on the formation of antigen-antibody aggregates. A quantitative approach was proposed for studying the mechanism of the antigen-antibody aggregate formation and for the analytical determination of various classes of immunoglobulins."}
{"id": "PMID:463424", "title": "[Comparative analysis of the interaction of protein A from Staphylococcus aureus with native, reduced and aggregated IgG].", "content": "In a comparative study the affinity of rabbit and human IgG, native, reduced and aggregated by various methods, to protein A obtained from the cell wall of Staphylococcus aureus was determined. For this purpose the method of the passive hemagglutination inhibition test was developed. The affinity to protein A was found to grow considerably after specific or non-specific IgG aggregation and to decrease by 60% after local damage affecting the structure of the IgG molecule waist as a result of the dissolution of the disulfide bond between its heavy chains. The problem of similarity between such effector properties of IgG as its ability to activate the complement system and to combine with protein A is considered, as well as the problem of the pathogenetic role of immune complexes bound with protein A.", "contents": "[Comparative analysis of the interaction of protein A from Staphylococcus aureus with native, reduced and aggregated IgG]. In a comparative study the affinity of rabbit and human IgG, native, reduced and aggregated by various methods, to protein A obtained from the cell wall of Staphylococcus aureus was determined. For this purpose the method of the passive hemagglutination inhibition test was developed. The affinity to protein A was found to grow considerably after specific or non-specific IgG aggregation and to decrease by 60% after local damage affecting the structure of the IgG molecule waist as a result of the dissolution of the disulfide bond between its heavy chains. The problem of similarity between such effector properties of IgG as its ability to activate the complement system and to combine with protein A is considered, as well as the problem of the pathogenetic role of immune complexes bound with protein A."}
{"id": "PMID:463425", "title": "[Quantitative indices of the activity and the electrophoretic characteristics of staphylococcal catalase].", "content": "The average levels of activity of intracellular and extracellular catalase were determined. The activity of intracellular catalase was shown to be significantly higher than that of extracellular catalase, the average level of activity of extracellular catalase being higher in S. aureus than in S. epidermidis. In most of the strains one zone of extracellular catalase and two molecular forms of intracellular catalase were revealed by means of polyacrylamide gel electrophoresis.", "contents": "[Quantitative indices of the activity and the electrophoretic characteristics of staphylococcal catalase]. The average levels of activity of intracellular and extracellular catalase were determined. The activity of intracellular catalase was shown to be significantly higher than that of extracellular catalase, the average level of activity of extracellular catalase being higher in S. aureus than in S. epidermidis. In most of the strains one zone of extracellular catalase and two molecular forms of intracellular catalase were revealed by means of polyacrylamide gel electrophoresis."}
{"id": "PMID:463426", "title": "[Depression and stimulation of immunologic memory by typhoid lipopolysaccharide and polysaccharide].", "content": "The effect of typhoid lipopolysaccharide (LPS) and polysaccharide (PS) on immunological memory in the system of IgM and IgG synthesis and rosette-forming cells was studied. When introduced into animals previously immunized with SRBC, PS stimulated, under certain conditions, immunological memory in the system of IgM synthesis and rosette-forming cells, while the injection of LPS induced only an insignificant stimulation of immunological memory. No stimulation in the system of IgG synthesis was observed after the injection of both LPS and PS. The suppression of immunological memory was noted in the animals receiving LPS as well as in those receiving PS the immunosuppressing effect produced by LPS was more pronounced.", "contents": "[Depression and stimulation of immunologic memory by typhoid lipopolysaccharide and polysaccharide]. The effect of typhoid lipopolysaccharide (LPS) and polysaccharide (PS) on immunological memory in the system of IgM and IgG synthesis and rosette-forming cells was studied. When introduced into animals previously immunized with SRBC, PS stimulated, under certain conditions, immunological memory in the system of IgM synthesis and rosette-forming cells, while the injection of LPS induced only an insignificant stimulation of immunological memory. No stimulation in the system of IgG synthesis was observed after the injection of both LPS and PS. The suppression of immunological memory was noted in the animals receiving LPS as well as in those receiving PS the immunosuppressing effect produced by LPS was more pronounced."}
{"id": "PMID:463427", "title": "[Morphology of the bifidobacteria by light and electron microscopy and their biological properties].", "content": "The morphology and the acid-producing, antagonistic and enzymatic activity of the strains of bifidobacteria isolated from the intestine of adults and children were studied. Bifidobacteria showed a considerable polymorphism, all the strains were antagonistically active. The strains isolated from adults were found to have greater acid-producing activity; the predominant species were B. longum and B. adolescentis, and in children B. bifidum were also isolated. The characteristic feature of B. longum strains was the presence of a slime-like layer and formations resembling bubbles around bacterial cells. The stains with greater physiological activity were found to have an extensive mesosomal complex, as well as a great number of volutin granules.", "contents": "[Morphology of the bifidobacteria by light and electron microscopy and their biological properties]. The morphology and the acid-producing, antagonistic and enzymatic activity of the strains of bifidobacteria isolated from the intestine of adults and children were studied. Bifidobacteria showed a considerable polymorphism, all the strains were antagonistically active. The strains isolated from adults were found to have greater acid-producing activity; the predominant species were B. longum and B. adolescentis, and in children B. bifidum were also isolated. The characteristic feature of B. longum strains was the presence of a slime-like layer and formations resembling bubbles around bacterial cells. The stains with greater physiological activity were found to have an extensive mesosomal complex, as well as a great number of volutin granules."}
{"id": "PMID:463428", "title": "[Sensitivity of different strains of the causative agent of plague to disinfectants].", "content": "Various strains of the plague microbe was found to have statistically significant differences in their sensitivity to phenol, formaldehyde and chloramine. These differences did not correlate with the origin, virulence and nutritional requirements of the plague strains. The sensitivity of the plague microbe to formaldehyde and phenol was found to be related to the permeability of its cell wall.", "contents": "[Sensitivity of different strains of the causative agent of plague to disinfectants]. Various strains of the plague microbe was found to have statistically significant differences in their sensitivity to phenol, formaldehyde and chloramine. These differences did not correlate with the origin, virulence and nutritional requirements of the plague strains. The sensitivity of the plague microbe to formaldehyde and phenol was found to be related to the permeability of its cell wall."}
{"id": "PMID:463429", "title": "[Gastrointestinal tract microflora in nonspecific ulcerative colitis].", "content": "The species and quantitative composition of the normal and conditionally pathogenic microflora of the stomach, the proximal portion of the small intestine, in material obtained by biopsy from the mucosa of this portion, and in feces was studied in 10 patients with non-specific ulcerative colitis. The study was made before and after treatment. Before treatment in most of the patients gastric juice and the proximal portion of the small intestine were found to be greatly contaminated with microflora commonly present in feces, and in fecal microflora sharp dysbiotic changes were observed. As a result of treatment the microflora was essentially normalized (with the exception of Proteus and bifidobacteria).", "contents": "[Gastrointestinal tract microflora in nonspecific ulcerative colitis]. The species and quantitative composition of the normal and conditionally pathogenic microflora of the stomach, the proximal portion of the small intestine, in material obtained by biopsy from the mucosa of this portion, and in feces was studied in 10 patients with non-specific ulcerative colitis. The study was made before and after treatment. Before treatment in most of the patients gastric juice and the proximal portion of the small intestine were found to be greatly contaminated with microflora commonly present in feces, and in fecal microflora sharp dysbiotic changes were observed. As a result of treatment the microflora was essentially normalized (with the exception of Proteus and bifidobacteria)."}
{"id": "PMID:463430", "title": "[Time of formation of traumatic intracranial hematomas].", "content": "Biochemical study of the methemoglobin concentration (60 cases) and histological examination (40 of intracranial hematomas removed in different periods after the trauma confirmed the assumption that the main volume of these hematomas forms within the first minutes and hours after the trauma as a rule. The curve of the dependence of the methemoglobin concentration in the hematoma on the time of its formation allows the period of time which had elapsed after the trauma to be determined.", "contents": "[Time of formation of traumatic intracranial hematomas]. Biochemical study of the methemoglobin concentration (60 cases) and histological examination (40 of intracranial hematomas removed in different periods after the trauma confirmed the assumption that the main volume of these hematomas forms within the first minutes and hours after the trauma as a rule. The curve of the dependence of the methemoglobin concentration in the hematoma on the time of its formation allows the period of time which had elapsed after the trauma to be determined."}
{"id": "PMID:463431", "title": "[Nature and outcomes of craniocerebral injury in a fall from a height].", "content": "The case records of 1,137 patients with trauma suffered as the result of a fall from a height were studied, 399 of them (35.1%) had a craniocerebral trauma. Isolated traumas with injury to the cranial bones and brain accounted for 74.4% of cases, multiple injuries (injuries to the cranial bones, brain, and facial skeleton) for 0.8%, and combined injuries (injuries (injuries to the skull, brain, weight-bearing and motor apparatus, and internal organs) for 24.8%. Severe craniocerebral trauma was encountered in 34.9% of the injured, trauma of moderate severity in 24.3%, and mild craniocerebral trauma in 40.8% of the injured. Death from a craniocerebral trauma suffered in a fall from a height occurred at the site of the accident in 17.2% of cases and in later periods at the hospital in 12.7% of cases.", "contents": "[Nature and outcomes of craniocerebral injury in a fall from a height]. The case records of 1,137 patients with trauma suffered as the result of a fall from a height were studied, 399 of them (35.1%) had a craniocerebral trauma. Isolated traumas with injury to the cranial bones and brain accounted for 74.4% of cases, multiple injuries (injuries to the cranial bones, brain, and facial skeleton) for 0.8%, and combined injuries (injuries (injuries to the skull, brain, weight-bearing and motor apparatus, and internal organs) for 24.8%. Severe craniocerebral trauma was encountered in 34.9% of the injured, trauma of moderate severity in 24.3%, and mild craniocerebral trauma in 40.8% of the injured. Death from a craniocerebral trauma suffered in a fall from a height occurred at the site of the accident in 17.2% of cases and in later periods at the hospital in 12.7% of cases."}
{"id": "PMID:463432", "title": "[Potentials of hyperbaric oxygenation in correcting central respiratory disorders in ischemic stroke].", "content": "Changes in central respiratory disorders under the effect of hyperbaric oxygenation (HBO) were studied in 29 patients in the acute stage of ischemic stroke. Spirography, tetrapolar rheography, and examination of the blood gas composition were carried out. Before HBO was begun, various disorders of the respiratory function were found (hyperventilation syndrome, periodical rhythms: Cheyne-Stokes respiration, alternating respiration, etc.). It was revealed that HBO session causes a normalizing effect on the respiratory function in central disorders of respiration: the Cheyne-Stokes rhythm was replaced at the end of the session by wave-like respiration which is biologically more adequate, the regimen of hyperventilation changed to normoventilation, etc. There was parallel improvement in the indices of central hemodynamics and the blood gas composition.", "contents": "[Potentials of hyperbaric oxygenation in correcting central respiratory disorders in ischemic stroke]. Changes in central respiratory disorders under the effect of hyperbaric oxygenation (HBO) were studied in 29 patients in the acute stage of ischemic stroke. Spirography, tetrapolar rheography, and examination of the blood gas composition were carried out. Before HBO was begun, various disorders of the respiratory function were found (hyperventilation syndrome, periodical rhythms: Cheyne-Stokes respiration, alternating respiration, etc.). It was revealed that HBO session causes a normalizing effect on the respiratory function in central disorders of respiration: the Cheyne-Stokes rhythm was replaced at the end of the session by wave-like respiration which is biologically more adequate, the regimen of hyperventilation changed to normoventilation, etc. There was parallel improvement in the indices of central hemodynamics and the blood gas composition."}
{"id": "PMID:463437", "title": "Gallstone ileus. A retrospective study.", "content": "Eight patients have been admitted with gallstone ileus (GSI) to the Herning Central Hospital during the period 1960--1977. All of the patients were subjected to enterolithotomy only. One patient died postoperatively. On follow-up examination 6 months to 11 1/2 years after operation, one patient complained of a recurrence of cholecystitis, but refused operation. The other patients had no biliary tract complaints. We still consider, due to the above, that enterolithotomy alone is a sufficient primary procedure, and that only in cases of new biliary tract complaints can cholecystectomy and closure of an eventual fistula be considered.", "contents": "Gallstone ileus. A retrospective study. Eight patients have been admitted with gallstone ileus (GSI) to the Herning Central Hospital during the period 1960--1977. All of the patients were subjected to enterolithotomy only. One patient died postoperatively. On follow-up examination 6 months to 11 1/2 years after operation, one patient complained of a recurrence of cholecystitis, but refused operation. The other patients had no biliary tract complaints. We still consider, due to the above, that enterolithotomy alone is a sufficient primary procedure, and that only in cases of new biliary tract complaints can cholecystectomy and closure of an eventual fistula be considered."}
{"id": "PMID:463433", "title": "[Dynamics of diencephalic-hypothalamic disorders after the removal of basal meningiomas].", "content": "Comprehensive dynamic examination of 27 patients after removal of basal meningiomas revealed some characteristic changes in the results of REG and EEG and in the neurological status. It was also found that the dynamics of the data studied differed with the course of the postoperative period. Some EEG and REG data of favourable prognostic importance were noted. The study of these data promotes objectivization of the course of the postoperative period and is thus also important in the choice of rational therapy in the postoperative period.", "contents": "[Dynamics of diencephalic-hypothalamic disorders after the removal of basal meningiomas]. Comprehensive dynamic examination of 27 patients after removal of basal meningiomas revealed some characteristic changes in the results of REG and EEG and in the neurological status. It was also found that the dynamics of the data studied differed with the course of the postoperative period. Some EEG and REG data of favourable prognostic importance were noted. The study of these data promotes objectivization of the course of the postoperative period and is thus also important in the choice of rational therapy in the postoperative period."}
{"id": "PMID:463434", "title": "[New method of draining a syringomyelitic cyst].", "content": "A new method of operative intervention for syringomyelia is described in which after the syringomyelia cyst is opened its permanent drainage is accomplished by introducing into its cavity the free end of an adjacent posterior root transected before-hand directly at the site of the funnel-shaped transition of the dura mater from the spinal cord onto the cuff. As a result, all movements of the spinal cord are made together with this root, which prevents the draining root from falling out of the cystic cavity and does not create conditions for it to inflict damage to the cystic walls and the surrounding cerebral tissue. The method was used in operations on 9 patients.", "contents": "[New method of draining a syringomyelitic cyst]. A new method of operative intervention for syringomyelia is described in which after the syringomyelia cyst is opened its permanent drainage is accomplished by introducing into its cavity the free end of an adjacent posterior root transected before-hand directly at the site of the funnel-shaped transition of the dura mater from the spinal cord onto the cuff. As a result, all movements of the spinal cord are made together with this root, which prevents the draining root from falling out of the cystic cavity and does not create conditions for it to inflict damage to the cystic walls and the surrounding cerebral tissue. The method was used in operations on 9 patients."}
{"id": "PMID:463438", "title": "Bile duct diameter and diagnostic reliability at cholangiography.", "content": "Cases of exploratory choledochotomy with widened bile duct as the indication have been assessed with respect to missed bile duct concretion in a retrospective study covering a period of five years. During this period 2,000 cholecystectomies were performed. The 110 cases, in which (a) pre-exploratory cholangiography was completely normal apart from a diameter of more than 10 mm, (b) exploratory choledochotomy was performed, and (c) secondary cholangiography had been carried out, were studied in detail. No calculi were demonstrable either at exploratory surgery or secondary cholangiography. With a good radiographic technique it does not seem likely that bile duct concretions would be missed in widened ducts at otherwise normal operative cholangiography--at any rate not with bile duct diameters up to 20 mm.", "contents": "Bile duct diameter and diagnostic reliability at cholangiography. Cases of exploratory choledochotomy with widened bile duct as the indication have been assessed with respect to missed bile duct concretion in a retrospective study covering a period of five years. During this period 2,000 cholecystectomies were performed. The 110 cases, in which (a) pre-exploratory cholangiography was completely normal apart from a diameter of more than 10 mm, (b) exploratory choledochotomy was performed, and (c) secondary cholangiography had been carried out, were studied in detail. No calculi were demonstrable either at exploratory surgery or secondary cholangiography. With a good radiographic technique it does not seem likely that bile duct concretions would be missed in widened ducts at otherwise normal operative cholangiography--at any rate not with bile duct diameters up to 20 mm."}
{"id": "PMID:463439", "title": "Interval appendectomy. A retrospective study.", "content": "79 patients were admitted with a periappendicular abscess at the Surgical Organic Department, Randers Centralsygehus in a 5-year-period from April 1, 1970 to April 1, 1975. Appendectomy \u00e0 froid was not made on 9 patients. A questionnaire in 7 patients showed no recurrence after an observation period of an average 2.4 years. Appendectomy \u00e0 froid was made on 70 patients with a complication rate of 13%. The operation could be regraded as quite unnecessary in 14%. Therefore appendectomy \u00e0 froid cannot be recommended as a routine operation, but all patients ought to be controlled until the palpable mass has disappeared, and only in cases of doubt and in symptomatic cases the patients ought to be operated. All patients over a certain age, 40 years for instance, ought to have performed a colon X-ray with double contrast or coloscopy to exclude a coecal cancer.", "contents": "Interval appendectomy. A retrospective study. 79 patients were admitted with a periappendicular abscess at the Surgical Organic Department, Randers Centralsygehus in a 5-year-period from April 1, 1970 to April 1, 1975. Appendectomy \u00e0 froid was not made on 9 patients. A questionnaire in 7 patients showed no recurrence after an observation period of an average 2.4 years. Appendectomy \u00e0 froid was made on 70 patients with a complication rate of 13%. The operation could be regraded as quite unnecessary in 14%. Therefore appendectomy \u00e0 froid cannot be recommended as a routine operation, but all patients ought to be controlled until the palpable mass has disappeared, and only in cases of doubt and in symptomatic cases the patients ought to be operated. All patients over a certain age, 40 years for instance, ought to have performed a colon X-ray with double contrast or coloscopy to exclude a coecal cancer."}
{"id": "PMID:463436", "title": "[Technic for surgical intervention in severe brain contusions and crushing].", "content": "Basal contusions of the frontal and temporal lobes of the brain, occurring as a contrecoup as a rule, are particularly severe and are marked by a high mortality. The 10-year practice of the Sklifosovsky Scientific Research Institute of Emergency Aid in the surgical management of cerebral contusion was aimed at the search for a less traumatic craniotomy approach and the most sparing and radical, within reasonable limits, intervention on the cerebral focus. Analysis of the work done enables us to choose the method described in the article, comprising extensive infratemporal craniotomy which excludes the frontal, temporal, and parietal, lobes, and to recommend combining intervention on the focus with internal decompression and active aspiration of the wound discharge from the cranial cavity in the first 24 hours after the operation. The illustrations supplied show the stages of the operation: linear skin incision, incision and separation of the temporal muscle, resection of the squama of the temporal and frontal bones down to the base of the skull. The operation may also be carried out as osteoplasty (sites for forming the trephination openings are shown in Fig. 1, a).", "contents": "[Technic for surgical intervention in severe brain contusions and crushing]. Basal contusions of the frontal and temporal lobes of the brain, occurring as a contrecoup as a rule, are particularly severe and are marked by a high mortality. The 10-year practice of the Sklifosovsky Scientific Research Institute of Emergency Aid in the surgical management of cerebral contusion was aimed at the search for a less traumatic craniotomy approach and the most sparing and radical, within reasonable limits, intervention on the cerebral focus. Analysis of the work done enables us to choose the method described in the article, comprising extensive infratemporal craniotomy which excludes the frontal, temporal, and parietal, lobes, and to recommend combining intervention on the focus with internal decompression and active aspiration of the wound discharge from the cranial cavity in the first 24 hours after the operation. The illustrations supplied show the stages of the operation: linear skin incision, incision and separation of the temporal muscle, resection of the squama of the temporal and frontal bones down to the base of the skull. The operation may also be carried out as osteoplasty (sites for forming the trephination openings are shown in Fig. 1, a)."}
{"id": "PMID:463441", "title": "Skeletal scintigraphy in the inital assessment of women with breast cancer.", "content": "The results of 99mTc bone scanning have been retrospectively analyzed on 467 women admitted with a newly diagnosed breast cancer. The outcome of skeletal scintigraphy has been studied with reference to various prognostic variables. 9.8% of the women exhibited a positive scintigram. We could not find any influence of the tumour position on the frequency of positive bone scans. Age in relation to the menopause affected the rate of positive scans in that about three times as many positive scans were recorded in post menopausal women than in those who had not reached the menopause. Clinical staging according to the TNM system as well as the histological status of the axillary nodes correlated well with the rate of scintigrams that indicated a disseminated cancer disease. The tumour size, as measured in the operation specimen, strongly affected the frequency of positive and suspicious scans in accord with the prognostic significance of this parameter. The role of skeletal scintigraphy for an adequate staging of breast cancer patients is discussed.", "contents": "Skeletal scintigraphy in the inital assessment of women with breast cancer. The results of 99mTc bone scanning have been retrospectively analyzed on 467 women admitted with a newly diagnosed breast cancer. The outcome of skeletal scintigraphy has been studied with reference to various prognostic variables. 9.8% of the women exhibited a positive scintigram. We could not find any influence of the tumour position on the frequency of positive bone scans. Age in relation to the menopause affected the rate of positive scans in that about three times as many positive scans were recorded in post menopausal women than in those who had not reached the menopause. Clinical staging according to the TNM system as well as the histological status of the axillary nodes correlated well with the rate of scintigrams that indicated a disseminated cancer disease. The tumour size, as measured in the operation specimen, strongly affected the frequency of positive and suspicious scans in accord with the prognostic significance of this parameter. The role of skeletal scintigraphy for an adequate staging of breast cancer patients is discussed."}
{"id": "PMID:463442", "title": "Active and inactive thyroid hormone levels in elective and acute surgery.", "content": "The changes in the plasma or serum concentration of thyrotrophin (TSH), thyroxine (T4), 3,5,3'-triiodothyronine (T3), 3,3',5-triiodothyronine (reverse T3, rT3) and cortisol were examined in patients undergoing elective cholecystectomy (n = 10) or acute laparotomy due to peritonitis (n = 11). TSH and T4 showed no essential changes in either group. T3 was reduced already during initiation of anaesthesia and continued to fall during and after surgery in both groups. In the peritonitis group, T3 was reduced already before medical intervention. rT3 displayed changes opposite to those of T3. An increase in cortisol peceded the changes in T3 and rT3 in both groups. T3 and rT3 returned towards normal levels when the patients recovered and resumed oral nutrition. It seems likely that both elective and acute uncomplicated surgery is accompanied by a transient reduction in the extrathyroidal production of the most active hormone, T3, and by a reciprocal increase in the levels of the virtually inactive rT3. The underlying mechanism behind this metabolic adaptation is unclear but may be related to adrenocortical activation and/or to changes in the mode of nutrition.", "contents": "Active and inactive thyroid hormone levels in elective and acute surgery. The changes in the plasma or serum concentration of thyrotrophin (TSH), thyroxine (T4), 3,5,3'-triiodothyronine (T3), 3,3',5-triiodothyronine (reverse T3, rT3) and cortisol were examined in patients undergoing elective cholecystectomy (n = 10) or acute laparotomy due to peritonitis (n = 11). TSH and T4 showed no essential changes in either group. T3 was reduced already during initiation of anaesthesia and continued to fall during and after surgery in both groups. In the peritonitis group, T3 was reduced already before medical intervention. rT3 displayed changes opposite to those of T3. An increase in cortisol peceded the changes in T3 and rT3 in both groups. T3 and rT3 returned towards normal levels when the patients recovered and resumed oral nutrition. It seems likely that both elective and acute uncomplicated surgery is accompanied by a transient reduction in the extrathyroidal production of the most active hormone, T3, and by a reciprocal increase in the levels of the virtually inactive rT3. The underlying mechanism behind this metabolic adaptation is unclear but may be related to adrenocortical activation and/or to changes in the mode of nutrition."}
{"id": "PMID:463443", "title": "Intrathoracic goitre: a review of 29 cases.", "content": "Twenty-nine patients suffering from intrathoracic goitre who were admitted to a thoracic surgical clinic were studied. Most patients had respiratory complaints but as many as 28% did not have any symptoms and their goitres were revealed on routine chest X-rays. Only seven patients were operated upon with a thoracic approach, usually a sternal split. All others were operated upon with a cervical collar incision. Three of the goitres were toxic and one malignant. Two of the patients died, one when inducing anaesthesia and the other of pulmonary embolism six days after the operation. General anaesthesia with endotracheal intubation is preferred when operating for intrathoracic goitre. The operation is started with a cervical collar incision and the patients prepared for a sternal split. Lateral thoracotomy may be necessary only when the mass is situated in the posterior mediastinum.", "contents": "Intrathoracic goitre: a review of 29 cases. Twenty-nine patients suffering from intrathoracic goitre who were admitted to a thoracic surgical clinic were studied. Most patients had respiratory complaints but as many as 28% did not have any symptoms and their goitres were revealed on routine chest X-rays. Only seven patients were operated upon with a thoracic approach, usually a sternal split. All others were operated upon with a cervical collar incision. Three of the goitres were toxic and one malignant. Two of the patients died, one when inducing anaesthesia and the other of pulmonary embolism six days after the operation. General anaesthesia with endotracheal intubation is preferred when operating for intrathoracic goitre. The operation is started with a cervical collar incision and the patients prepared for a sternal split. Lateral thoracotomy may be necessary only when the mass is situated in the posterior mediastinum."}
{"id": "PMID:463444", "title": "Optimal regimen of low-dose heparin prophylaxis in gastrointestinal surgery.", "content": "Four low-dose heparin regimens were compared with respect to the postoperative frequency of deep venous thrombosis (DVT) diagnosed with the 125I-fibrinogen method and other thrombosis variables as well as to peroperative and postoperative bleeding. The study comprised 204 patients undergoing gastrointestinal surgery who received 5,000 IU of either calcium heparin or sodium heparin (Vitrum AB) subcutaneously 2 hours before the operation and either every 8 or every 12th hours afterwards for 6--8 days. DVT was diagnosed in 17 and 16% of the patients who received calcium heparin every 8th or 12th hour respectively and in 11 and 10% respectively of those given sodium heparin every 8th or 12th hour. The differences in the incidence of DVT between the four groups were not statistically significant. An 8-hourly regimen was not accompanied by more bleeding incidents than a 12-hourly regimen. Sodium heparin was associated with a significantly increased number of patients who required blood transfusion and had more bruising at the injection site. It is concluded that the safest, most practical and still effective of the four investigated low-dose heparin regimens is calcium heparin 5,000 IU/ml administered 2 hours before the operation and every 12 hours afterwards for the first postoperative week.", "contents": "Optimal regimen of low-dose heparin prophylaxis in gastrointestinal surgery. Four low-dose heparin regimens were compared with respect to the postoperative frequency of deep venous thrombosis (DVT) diagnosed with the 125I-fibrinogen method and other thrombosis variables as well as to peroperative and postoperative bleeding. The study comprised 204 patients undergoing gastrointestinal surgery who received 5,000 IU of either calcium heparin or sodium heparin (Vitrum AB) subcutaneously 2 hours before the operation and either every 8 or every 12th hours afterwards for 6--8 days. DVT was diagnosed in 17 and 16% of the patients who received calcium heparin every 8th or 12th hour respectively and in 11 and 10% respectively of those given sodium heparin every 8th or 12th hour. The differences in the incidence of DVT between the four groups were not statistically significant. An 8-hourly regimen was not accompanied by more bleeding incidents than a 12-hourly regimen. Sodium heparin was associated with a significantly increased number of patients who required blood transfusion and had more bruising at the injection site. It is concluded that the safest, most practical and still effective of the four investigated low-dose heparin regimens is calcium heparin 5,000 IU/ml administered 2 hours before the operation and every 12 hours afterwards for the first postoperative week."}
{"id": "PMID:463445", "title": "Hemodynamic evaluation of the Carpentier-Edwards bioprosthesis in the aortic position.", "content": "The Carpentier-Edwards bioprosthesis is a glutaraldehyde-fixed porcine xenograft with a fully flexible thin-walled stent. Cardiac catheterization studies were performed in 17 patients to evaluate use of this valve in the aortic position. Hemodynamic studies established a mean peak gradient across the prosthesis of 19 mm Hg (range 5 to 65). The mean effective orifice area was calculated to be 1.6 cm2 (range 0.8 to 3.3). All patients demonstrated an improvement in functional class after operation. Mean left ventricular ejection fraction increased from 51 +/- 16 to 68 +/- 9 percent (P less than 0.004) in eight patients operated on for aortic stenosis, but was not significantly changed in patients operated on for aortic insufficiency. Hemodynamic comparison of the Carpentier-Edwards bioprosthesis with the standard Hancock xenograft showed similar effective orifice areas for the 23 and 25 mm diameter valves. In two patients studied the 21 mm Carpentier valve demonstrated a greater effective orifice area than that previously reported for the standard Hancock xenograft. The Carpentier-Edwards bioprosthesis affords both clinical and hemodynamic improvement when used in the aortic position and may allow improved effective orifice area when used in the smaller aortic root.", "contents": "Hemodynamic evaluation of the Carpentier-Edwards bioprosthesis in the aortic position. The Carpentier-Edwards bioprosthesis is a glutaraldehyde-fixed porcine xenograft with a fully flexible thin-walled stent. Cardiac catheterization studies were performed in 17 patients to evaluate use of this valve in the aortic position. Hemodynamic studies established a mean peak gradient across the prosthesis of 19 mm Hg (range 5 to 65). The mean effective orifice area was calculated to be 1.6 cm2 (range 0.8 to 3.3). All patients demonstrated an improvement in functional class after operation. Mean left ventricular ejection fraction increased from 51 +/- 16 to 68 +/- 9 percent (P less than 0.004) in eight patients operated on for aortic stenosis, but was not significantly changed in patients operated on for aortic insufficiency. Hemodynamic comparison of the Carpentier-Edwards bioprosthesis with the standard Hancock xenograft showed similar effective orifice areas for the 23 and 25 mm diameter valves. In two patients studied the 21 mm Carpentier valve demonstrated a greater effective orifice area than that previously reported for the standard Hancock xenograft. The Carpentier-Edwards bioprosthesis affords both clinical and hemodynamic improvement when used in the aortic position and may allow improved effective orifice area when used in the smaller aortic root."}
{"id": "PMID:463446", "title": "Effect of androgen on growth hormone secretion and growth in boys with short stature.", "content": "The response of plasma growth hormone (GH) to insulin-induced hypoglycaemia (IIH) and arginine infusion (AI) was studied in 22 young males (ages 8 to 17 years) with short atature and absent or delayed sexual maturation, before and after androgen administration. During initial evaluation, 5 patients had blunted GH response to IIH, 12 responded subnormally to AI and 4 failed to respond normally to either stimulus. These same studies were repeated in each patient following androgen administration. The source of androgen was as follows: a) 5 days of testosterone propionate (25 mg intramuscularly daily) in 20 patients. b) Methyltestosterone, 10 mg t.i.d. orally for four days in the other 2 subjects. In almost every case, androgen administration resulted in raising the levels of fasting GH and enhancement of the GH responses to IIH and AI was observed. Patients manifesting subnormal GH responses to these stimuli before androgen consistently demonstrated a normal response when challenged with identical stimuli during androgen administration. Growth velocities during the year following these studies were significantly increased in most instances and the growth spurts correlated well with the progression of sexual maturation. Sustained improvement in the GH responses to IIH and AI were uniformly observed in 3 patients when repetitive studies were performed 8 to 12 months later during spontaneous advancing sexual development. The results indicate that brief androgen administration can be helpful in delineating the cause of growth retardation in boys with short stature and delayed sexual maturation, particularly when the diagnosis of isolated growth hormone deficiency is suspected. They also offer prognostic value in determining growth potential in this same group of young males.", "contents": "Effect of androgen on growth hormone secretion and growth in boys with short stature. The response of plasma growth hormone (GH) to insulin-induced hypoglycaemia (IIH) and arginine infusion (AI) was studied in 22 young males (ages 8 to 17 years) with short atature and absent or delayed sexual maturation, before and after androgen administration. During initial evaluation, 5 patients had blunted GH response to IIH, 12 responded subnormally to AI and 4 failed to respond normally to either stimulus. These same studies were repeated in each patient following androgen administration. The source of androgen was as follows: a) 5 days of testosterone propionate (25 mg intramuscularly daily) in 20 patients. b) Methyltestosterone, 10 mg t.i.d. orally for four days in the other 2 subjects. In almost every case, androgen administration resulted in raising the levels of fasting GH and enhancement of the GH responses to IIH and AI was observed. Patients manifesting subnormal GH responses to these stimuli before androgen consistently demonstrated a normal response when challenged with identical stimuli during androgen administration. Growth velocities during the year following these studies were significantly increased in most instances and the growth spurts correlated well with the progression of sexual maturation. Sustained improvement in the GH responses to IIH and AI were uniformly observed in 3 patients when repetitive studies were performed 8 to 12 months later during spontaneous advancing sexual development. The results indicate that brief androgen administration can be helpful in delineating the cause of growth retardation in boys with short stature and delayed sexual maturation, particularly when the diagnosis of isolated growth hormone deficiency is suspected. They also offer prognostic value in determining growth potential in this same group of young males."}
{"id": "PMID:463447", "title": "Inhibition of prolactin and lactation by methylergometrine hydrogenmaleate.", "content": "Reports on the effect of methylergometrine hydrogenmaleate (MEM) on prolactin (PRL) secretion and lactation are still controversial. Therefore a prospective study was designed to follow the influence of MEM on post-partum PRL levels and milk production. MEM was given orally to 30 post-partum women from day 1 to day 7 in a daily dose of 0.6 mg. Thirty untreated women served as control. PRL plasma levels and milk yield were compared at day 1, 3 and 7. PRL levels were significantly lower in the treated group at day 7 (P less than 0.01), while the difference at day 3 was statistically not significant. Milk yield was significantly reduced at day 3 (P less than 0.05) and day 7 (P less than 0.01). These results indicate PRL inhibiting properties of MEM resulting consequently in reduced lactation.", "contents": "Inhibition of prolactin and lactation by methylergometrine hydrogenmaleate. Reports on the effect of methylergometrine hydrogenmaleate (MEM) on prolactin (PRL) secretion and lactation are still controversial. Therefore a prospective study was designed to follow the influence of MEM on post-partum PRL levels and milk production. MEM was given orally to 30 post-partum women from day 1 to day 7 in a daily dose of 0.6 mg. Thirty untreated women served as control. PRL plasma levels and milk yield were compared at day 1, 3 and 7. PRL levels were significantly lower in the treated group at day 7 (P less than 0.01), while the difference at day 3 was statistically not significant. Milk yield was significantly reduced at day 3 (P less than 0.05) and day 7 (P less than 0.01). These results indicate PRL inhibiting properties of MEM resulting consequently in reduced lactation."}
{"id": "PMID:463448", "title": "A sensitive and specific in vitro bioassay method for the measurement of follicle-stimulating hormone activity.", "content": "An in vitro bioassay method for hFSH is presented. The method is based on the principles previously described by Dorrington et al. (1976b) and involves the assay of oestradiol produced from 19-hydroxyandrostenedione by dispersed Sertoli cells of 10-day old rats when cultured in the presence of graded doses of FSH. Using the 1st International Reference Preparation for human pituitary gonadotrophins (FSH and LH/ICSH) for bioassay (code no. 69/104) as standard, the useful range of the method is from 0.5 to 32 mIU/chamber (2 to 128 mIU/ml). The sensitivity of the method is 0.5 mIU/chamger. The mean index of precision (lambda) obtained from 16 multiple assays over 2 or 3 dose levels was 0.084. Parallelism was obtained between the 69/104 preparation and all preparations under study. The practicability of the proposed assay method is such that 15 preparations at 3 dose levels can be assayed by one person in 3 days. The specificity of the assay was investigated by determining the FSH activity in the following preparations: hFSH alpha- and beta-subunits, hLH, hCG, hTSH, ACTH, human growth hormone (hGH) human prolactin (hPRL) and luteinizing hormone-releasing hormone (LH-RH). The ACTH, hGH, hPRL and LH-RH preparations studied showed no detectable FSH activity in the assay. In the remaining preparations very low levels of FSH activity were found, corresponding to 0.004 to 0.6% of the weight of these preparations when compared with a highly purified hFSH preparation, suggesting that the method is specific for FSH. The possible synergistic or antagonistic influence of the above preparations when assayed in the presence of the 69/104 preparation was also assessed. No evidence of a synergistic or antogonistic effect was found. The assay of the hFSH potencies of a limited number of hFSH preparations of varying purity by the proposed in vitro bioassay, an hFSH radioreceptor method and an hFSH specific radioimmunoassay technique revealed that - although the relationship of the various potencies obtained with each method showed a close agreement - the bioassays yielded the highest potency estimates, and the radioimmunoassays the lowest ones. Since the proposed bioassay method is sensitive and considered to be specific for hFSH activity, it provides a suitable basis for the assessment of the specificity of other in vitro methods (radioreceptor and radioimmunoassay) currently used for detecting low levels of FSH activity.", "contents": "A sensitive and specific in vitro bioassay method for the measurement of follicle-stimulating hormone activity. An in vitro bioassay method for hFSH is presented. The method is based on the principles previously described by Dorrington et al. (1976b) and involves the assay of oestradiol produced from 19-hydroxyandrostenedione by dispersed Sertoli cells of 10-day old rats when cultured in the presence of graded doses of FSH. Using the 1st International Reference Preparation for human pituitary gonadotrophins (FSH and LH/ICSH) for bioassay (code no. 69/104) as standard, the useful range of the method is from 0.5 to 32 mIU/chamber (2 to 128 mIU/ml). The sensitivity of the method is 0.5 mIU/chamger. The mean index of precision (lambda) obtained from 16 multiple assays over 2 or 3 dose levels was 0.084. Parallelism was obtained between the 69/104 preparation and all preparations under study. The practicability of the proposed assay method is such that 15 preparations at 3 dose levels can be assayed by one person in 3 days. The specificity of the assay was investigated by determining the FSH activity in the following preparations: hFSH alpha- and beta-subunits, hLH, hCG, hTSH, ACTH, human growth hormone (hGH) human prolactin (hPRL) and luteinizing hormone-releasing hormone (LH-RH). The ACTH, hGH, hPRL and LH-RH preparations studied showed no detectable FSH activity in the assay. In the remaining preparations very low levels of FSH activity were found, corresponding to 0.004 to 0.6% of the weight of these preparations when compared with a highly purified hFSH preparation, suggesting that the method is specific for FSH. The possible synergistic or antagonistic influence of the above preparations when assayed in the presence of the 69/104 preparation was also assessed. No evidence of a synergistic or antogonistic effect was found. The assay of the hFSH potencies of a limited number of hFSH preparations of varying purity by the proposed in vitro bioassay, an hFSH radioreceptor method and an hFSH specific radioimmunoassay technique revealed that - although the relationship of the various potencies obtained with each method showed a close agreement - the bioassays yielded the highest potency estimates, and the radioimmunoassays the lowest ones. Since the proposed bioassay method is sensitive and considered to be specific for hFSH activity, it provides a suitable basis for the assessment of the specificity of other in vitro methods (radioreceptor and radioimmunoassay) currently used for detecting low levels of FSH activity."}
{"id": "PMID:463449", "title": "Characterization of specific antisera to native human luteinizing hormone.", "content": "Antisera against highly purified native hLH (10 000 IU/mg = 0.5 mg hLH/mg protein) were raised in 4 rabbits. The antisera were invariably of high titre and high avidity. They were purified by affinity chromatography on a column with hCG-Sepharose 4 B. Before and after adsorption the antisera were tested in a double antibody radioimmunoassay. Before adsorption hCG cross-reacted in all antisera, but in none were the inhibition curves identical. No such cross-reaction remained after the affinity chromatography. These purified antisera made it possible to develop highly specific and sensitive assays for measuring native hLH. We conclude from studies of the effect of native glycoprotein hormones and their subunits that there are probably differences in the antigenic sites of the complete hLH molecule and in the isolated beta-subunit of hLH. The investigation also indicates that even highly purified preparations of hTSH may be contaminated with hLH and that the preparations of hLH-subunits may contain a certain amount of the native hormone.", "contents": "Characterization of specific antisera to native human luteinizing hormone. Antisera against highly purified native hLH (10 000 IU/mg = 0.5 mg hLH/mg protein) were raised in 4 rabbits. The antisera were invariably of high titre and high avidity. They were purified by affinity chromatography on a column with hCG-Sepharose 4 B. Before and after adsorption the antisera were tested in a double antibody radioimmunoassay. Before adsorption hCG cross-reacted in all antisera, but in none were the inhibition curves identical. No such cross-reaction remained after the affinity chromatography. These purified antisera made it possible to develop highly specific and sensitive assays for measuring native hLH. We conclude from studies of the effect of native glycoprotein hormones and their subunits that there are probably differences in the antigenic sites of the complete hLH molecule and in the isolated beta-subunit of hLH. The investigation also indicates that even highly purified preparations of hTSH may be contaminated with hLH and that the preparations of hLH-subunits may contain a certain amount of the native hormone."}
{"id": "PMID:463450", "title": "Measurement of circulating 1,25-dihydroxyvitamin D in man. Changes in serum concentrations during treatment with 1 alpha-hydroxycholecalciferol.", "content": "A sensitive assay is described for measurement of circulating 1,25-dihydroxyvitamin D (1,25-(OH)2D) using rachitic chick intestinal cytosol binding protein. The metabolite was extracted from serum by diethyl ether and chromotographed on a Sephadex LH-20 column followed by high-pressure liquid chromatography. The concentration of 1,25-(OH)2D was then measured by a competitive protein-binding assay. The binding capacity of the protein was unchanged for 4 months. The inter- and intra-assay variations were both 11%. The standard curve was useful at serum concentrations ranging from 14 to 395 pmol/l (6 to 165 pg/ml). The normal mean concentration was 79.6 +/- 36.8 pmol/l (33.1 +/- 15.3 pg/ml). The serum 1,25-(OH)2D was studied in elderly subjects following administration of 1 alpha-hydroxycholecalciferol (1 alpha-OHD3) at different doses. Large variations occurred indicating individual rates of metabolism of 1,25-(OH)2D.", "contents": "Measurement of circulating 1,25-dihydroxyvitamin D in man. Changes in serum concentrations during treatment with 1 alpha-hydroxycholecalciferol. A sensitive assay is described for measurement of circulating 1,25-dihydroxyvitamin D (1,25-(OH)2D) using rachitic chick intestinal cytosol binding protein. The metabolite was extracted from serum by diethyl ether and chromotographed on a Sephadex LH-20 column followed by high-pressure liquid chromatography. The concentration of 1,25-(OH)2D was then measured by a competitive protein-binding assay. The binding capacity of the protein was unchanged for 4 months. The inter- and intra-assay variations were both 11%. The standard curve was useful at serum concentrations ranging from 14 to 395 pmol/l (6 to 165 pg/ml). The normal mean concentration was 79.6 +/- 36.8 pmol/l (33.1 +/- 15.3 pg/ml). The serum 1,25-(OH)2D was studied in elderly subjects following administration of 1 alpha-hydroxycholecalciferol (1 alpha-OHD3) at different doses. Large variations occurred indicating individual rates of metabolism of 1,25-(OH)2D."}
{"id": "PMID:463451", "title": "Inhibition of diphosphonate-blocked bone mineralization. Evidence that calcitonin promotes mineralization.", "content": "There is much experimental evidence which indicates that calcitonin in hibits bone mineral resorption, but there are few data available in support of the proposal that calcitonin may also promote mineralization. Ethane-1-hydroxy-1,1-diphosphonate (EHDP) administered to immature rats inhibited mineralization as evidenced by widened tibial epiphyseal plates and decreased bone ash to dry weight ratios. Concurrent dosing with salmon calcitonin (SCT) prevented or reversed the EHDP-blocked mineralization in a dose dependent manner. Administration of SCT during the period after EHDP treatment significantly improved mineralization of tibial epiphyseal plates as shown by plate width narrowing and increased uptake of radioactive calcium. These results suggest that SCT increased mineralization in EHDP-treated rats, and provide supportive evidence for the proposal that calcitonin may also promote mineralization, in addition to its well known ability to inhibit bone mineral resorption.", "contents": "Inhibition of diphosphonate-blocked bone mineralization. Evidence that calcitonin promotes mineralization. There is much experimental evidence which indicates that calcitonin in hibits bone mineral resorption, but there are few data available in support of the proposal that calcitonin may also promote mineralization. Ethane-1-hydroxy-1,1-diphosphonate (EHDP) administered to immature rats inhibited mineralization as evidenced by widened tibial epiphyseal plates and decreased bone ash to dry weight ratios. Concurrent dosing with salmon calcitonin (SCT) prevented or reversed the EHDP-blocked mineralization in a dose dependent manner. Administration of SCT during the period after EHDP treatment significantly improved mineralization of tibial epiphyseal plates as shown by plate width narrowing and increased uptake of radioactive calcium. These results suggest that SCT increased mineralization in EHDP-treated rats, and provide supportive evidence for the proposal that calcitonin may also promote mineralization, in addition to its well known ability to inhibit bone mineral resorption."}
{"id": "PMID:463453", "title": "Ethanol elimination in regenerating rat liver: the roles of alcohol dehydrogenase and acetaldehyde.", "content": "The rate of ethanol elimination in vivo was studied with rats in which the energy consumption of the liver was increased by partial hepatectomy. Immediately after partial hepatectomy the activity of alcohol dehydrogenase in the liver remnant was not changed from that of the livers of sham-operated controls, but the rate of ethanol removal was significantly faster. Twenty-four h after the partial hepatectomy the activity of alcohol dehydrogenase was only 48 % of the activity measured in unoperated control rats. Therefore it is concluded that in normal liver the activity of ADH is in excess. In partially hepatectomized rats the rate of ethanol elimination was linearly correlated with the activity of alcohol dehydrogenase, which suggests that when the rate of NADH reoxidation is markedly increased, as in regenerating rat liver, the rate of ethanol elimination may be limited by the activity of alcohol dehydrogenase. The activity of aldehyde dehydrogenase and the concentration of acetaldehyde in the tail blood were not significantly changed from the level of unoperated rats during oxidation of ethanol.", "contents": "Ethanol elimination in regenerating rat liver: the roles of alcohol dehydrogenase and acetaldehyde. The rate of ethanol elimination in vivo was studied with rats in which the energy consumption of the liver was increased by partial hepatectomy. Immediately after partial hepatectomy the activity of alcohol dehydrogenase in the liver remnant was not changed from that of the livers of sham-operated controls, but the rate of ethanol removal was significantly faster. Twenty-four h after the partial hepatectomy the activity of alcohol dehydrogenase was only 48 % of the activity measured in unoperated control rats. Therefore it is concluded that in normal liver the activity of ADH is in excess. In partially hepatectomized rats the rate of ethanol elimination was linearly correlated with the activity of alcohol dehydrogenase, which suggests that when the rate of NADH reoxidation is markedly increased, as in regenerating rat liver, the rate of ethanol elimination may be limited by the activity of alcohol dehydrogenase. The activity of aldehyde dehydrogenase and the concentration of acetaldehyde in the tail blood were not significantly changed from the level of unoperated rats during oxidation of ethanol."}
{"id": "PMID:463455", "title": "[Presence of extracranial cerebral arterial lesions in patients undergoing open heart surgery: a planned approach to a single stage operation (author's transl)].", "content": "The combined approach of extracranial arterial lesions in patients undergoing open heart surgery decreases the high cardiac and neurological morbidity and mortality presented by patients operated sequentially. Besides the anatomically sound simultaneous approach of arch lesions and heart surgery, both to be operated by median sternotomy, the authors present their criteria used in the selection of neurologically symptomatic and asymptomatic patients with carotid and vertebral disease to be operated simultaneously. Results in 17 personal cases are presented.", "contents": "[Presence of extracranial cerebral arterial lesions in patients undergoing open heart surgery: a planned approach to a single stage operation (author's transl)]. The combined approach of extracranial arterial lesions in patients undergoing open heart surgery decreases the high cardiac and neurological morbidity and mortality presented by patients operated sequentially. Besides the anatomically sound simultaneous approach of arch lesions and heart surgery, both to be operated by median sternotomy, the authors present their criteria used in the selection of neurologically symptomatic and asymptomatic patients with carotid and vertebral disease to be operated simultaneously. Results in 17 personal cases are presented."}
{"id": "PMID:463456", "title": "[Revascularization of the extracranial carotid-, vertebral- and subclavian arteries in a small peripheral hospital (author's transl)].", "content": "A total of 21 procedures were performed on a group of 17 patients, 13 of them more than 65 years old, for mixed intra- and extracranial vessel disease. Ten had recovered from a frank stroke. Thirteen of them underwent one procedure, three two procedures and one a total of three revascularizations. Eighteen carotid endarterectomies, without patch angioplasty were performed. The determinant factor whether or not to use an intraluminal shunt was the peroperatory encephalographic monitoring by a neurologist. The shunt was used any time significant EEG changes occurred during a test clamping period of five minutes. The other procedures included a vertebral artery endarterectomy with patch angioplasty, a subclavian- subclavian vein by-pass, as well as a common carotid artery resection for elongation and kinking of the internal carotid artery combined with marked stenosis. The mortality was nihil. Postoperatively all patients did well with an improved mental and neurological status.", "contents": "[Revascularization of the extracranial carotid-, vertebral- and subclavian arteries in a small peripheral hospital (author's transl)]. A total of 21 procedures were performed on a group of 17 patients, 13 of them more than 65 years old, for mixed intra- and extracranial vessel disease. Ten had recovered from a frank stroke. Thirteen of them underwent one procedure, three two procedures and one a total of three revascularizations. Eighteen carotid endarterectomies, without patch angioplasty were performed. The determinant factor whether or not to use an intraluminal shunt was the peroperatory encephalographic monitoring by a neurologist. The shunt was used any time significant EEG changes occurred during a test clamping period of five minutes. The other procedures included a vertebral artery endarterectomy with patch angioplasty, a subclavian- subclavian vein by-pass, as well as a common carotid artery resection for elongation and kinking of the internal carotid artery combined with marked stenosis. The mortality was nihil. Postoperatively all patients did well with an improved mental and neurological status."}
{"id": "PMID:463454", "title": "[Treatment of carotid artery arteriosclerosis 1966 to 1976. Early and late results (author's transl)].", "content": "Early and late results of surgery for carotid arteriosclerosis between 1966 and 1976 were reviewed in 154 patients (192 operations). The percentages of excellent and good early results were 96.7% (stage 0), 91.3% (stage I), 46.6 (stage II) and 51.6% (stage III). Overall mortality was 9.1% within the month (3.8% as neurovascular risk). In late results, out of 140 survivors, the percentages of excellent and good results were 69% in stage 0, 59.7% in stage I, 45.5% in stage II and 56.7% in stage III, the mean delay being 40.5 months. There were 3.6% cases of neurological disorders (0.7% corresponding to the side of the operated carotid artery), 7.1% of vertigo and arterial hypertension, 33 (new) deaths (23.6%) occurred after a mean 31.3 months, of which 16 resulted from some cardiovascular trouble. The cumulative survival curves parallel those of the normal population showing the protection yielded by the operation and the decrease in neurovascular risk.", "contents": "[Treatment of carotid artery arteriosclerosis 1966 to 1976. Early and late results (author's transl)]. Early and late results of surgery for carotid arteriosclerosis between 1966 and 1976 were reviewed in 154 patients (192 operations). The percentages of excellent and good early results were 96.7% (stage 0), 91.3% (stage I), 46.6 (stage II) and 51.6% (stage III). Overall mortality was 9.1% within the month (3.8% as neurovascular risk). In late results, out of 140 survivors, the percentages of excellent and good results were 69% in stage 0, 59.7% in stage I, 45.5% in stage II and 56.7% in stage III, the mean delay being 40.5 months. There were 3.6% cases of neurological disorders (0.7% corresponding to the side of the operated carotid artery), 7.1% of vertigo and arterial hypertension, 33 (new) deaths (23.6%) occurred after a mean 31.3 months, of which 16 resulted from some cardiovascular trouble. The cumulative survival curves parallel those of the normal population showing the protection yielded by the operation and the decrease in neurovascular risk."}
{"id": "PMID:463459", "title": "[Five year follow-up of 104 cardial artery deobliteration (author's transl)].", "content": "From December 1, 1964 to December 31, 1977, 613 patients underwent 699 operations for stenotic lesions of the internal carotid artery. One hundred and four of these cases, operated before December 31, 1972, were reevaluated at 5 years. These include 9 patients stage 0 (asymptomatic), 45 patients stage I (transitory), 8 patients stage II (progressive), 22 stage III mild (mild deficiencies), and 20 stage III severe (severe deficiencies). The overall 5 year mortality is 34% with a survival rate of 65%, steadily decreasing from 87% in the first year. There is a fundamental difference to be made between results in stages 0, I, mild-III and the other, for immediate and for 5 year mortality: 19% versus 67%. The rate of local recurrences is low: 3% of patients operated on the same side. In contrast, 11 subjects underwent a bilateral procedure. Carotidal endarteriectomy improves long term survival only moderately, mainly as a result of coronary disease. However it has an undisputable effect in diminishing neurological accidents.", "contents": "[Five year follow-up of 104 cardial artery deobliteration (author's transl)]. From December 1, 1964 to December 31, 1977, 613 patients underwent 699 operations for stenotic lesions of the internal carotid artery. One hundred and four of these cases, operated before December 31, 1972, were reevaluated at 5 years. These include 9 patients stage 0 (asymptomatic), 45 patients stage I (transitory), 8 patients stage II (progressive), 22 stage III mild (mild deficiencies), and 20 stage III severe (severe deficiencies). The overall 5 year mortality is 34% with a survival rate of 65%, steadily decreasing from 87% in the first year. There is a fundamental difference to be made between results in stages 0, I, mild-III and the other, for immediate and for 5 year mortality: 19% versus 67%. The rate of local recurrences is low: 3% of patients operated on the same side. In contrast, 11 subjects underwent a bilateral procedure. Carotidal endarteriectomy improves long term survival only moderately, mainly as a result of coronary disease. However it has an undisputable effect in diminishing neurological accidents."}
{"id": "PMID:463458", "title": "[Techniques for the diagnosis of cerebral ischemia (author's transl)].", "content": "The various techniques for the diagnosis of cerebral ischemia are reviewed. The methods for investigating the large cervical arteries are considered (angiography, ultrasonic D\u00f6ppler technique, carotid compression test) as well as those concerned with intracranial circulation. The latter are divided in \"functional\" methods (Rheoencephalography cerebral blood flow measurements with Xenon or with microspheres) and \"morphological\" methods (Brain scintigraphy and CAT scan). The respective values of the various methods are estimated in a personal series of patients.", "contents": "[Techniques for the diagnosis of cerebral ischemia (author's transl)]. The various techniques for the diagnosis of cerebral ischemia are reviewed. The methods for investigating the large cervical arteries are considered (angiography, ultrasonic D\u00f6ppler technique, carotid compression test) as well as those concerned with intracranial circulation. The latter are divided in \"functional\" methods (Rheoencephalography cerebral blood flow measurements with Xenon or with microspheres) and \"morphological\" methods (Brain scintigraphy and CAT scan). The respective values of the various methods are estimated in a personal series of patients."}
{"id": "PMID:463460", "title": "[Cerebral revascularization by extra-intra-cranial arterial anastomosis. Technique and results (author's transl)].", "content": "Extra-intra-cranial anastomosis introduced in 1967 by Yasargil and Donaghy consists in using a branch of the external carotid artery to anastomose it on one of the cerebral cortex. This procedure is indicated when there exists an obliterating lesion not in reach of a local technique. On clinical grounds two situations may exist: --the first is a regressive ischemic episod and the operation is then preventive; --the second is a confirmed deficit, non regressive: thecurative value of such an operation must then be discussed. The author reports his 5 year experience: 51 patients were operated amounting to 60 anastomoses. Eighty percent of the operated patients have a patent anastomosis. The operative risk is small (1 death, 1 worsening). The clinical criteria are discussed (Acta chir. belg., 1979, 78, 85-93).", "contents": "[Cerebral revascularization by extra-intra-cranial arterial anastomosis. Technique and results (author's transl)]. Extra-intra-cranial anastomosis introduced in 1967 by Yasargil and Donaghy consists in using a branch of the external carotid artery to anastomose it on one of the cerebral cortex. This procedure is indicated when there exists an obliterating lesion not in reach of a local technique. On clinical grounds two situations may exist: --the first is a regressive ischemic episod and the operation is then preventive; --the second is a confirmed deficit, non regressive: thecurative value of such an operation must then be discussed. The author reports his 5 year experience: 51 patients were operated amounting to 60 anastomoses. Eighty percent of the operated patients have a patent anastomosis. The operative risk is small (1 death, 1 worsening). The clinical criteria are discussed (Acta chir. belg., 1979, 78, 85-93)."}
{"id": "PMID:463461", "title": "[One stage operation of bilateral carotid lesions (author's transl)].", "content": "Bilateral one stage carotid endarteriectomy was performed in 11 patients presenting with bilateral symptomatics stenoses without neurological nor cardiac major complications. We only found one such experience in the literature (1). The choice of a simultaneous procedure is based solely on electroencephalogram analysis and on the residual pressure after the test of carotid occlusion; the side where occlusion is tolerated and/or where the residual pressure is highest, is operated first. This simultaneous bilateral procedure carries the theoretical risk of ischemia. It has the advantage of decreasing the cardiac complications and the risk of neurological deficit and at the same token avoids intercurrent controlateral thrombosis and recurrent symptoms between two non-simultaneous procedures. In cases of symptomatic lesions, the indication of surgery is evident; for asymptomatic lesions we only consider a simultaneous procedure in special cases where there exists a threat of neurological complications.", "contents": "[One stage operation of bilateral carotid lesions (author's transl)]. Bilateral one stage carotid endarteriectomy was performed in 11 patients presenting with bilateral symptomatics stenoses without neurological nor cardiac major complications. We only found one such experience in the literature (1). The choice of a simultaneous procedure is based solely on electroencephalogram analysis and on the residual pressure after the test of carotid occlusion; the side where occlusion is tolerated and/or where the residual pressure is highest, is operated first. This simultaneous bilateral procedure carries the theoretical risk of ischemia. It has the advantage of decreasing the cardiac complications and the risk of neurological deficit and at the same token avoids intercurrent controlateral thrombosis and recurrent symptoms between two non-simultaneous procedures. In cases of symptomatic lesions, the indication of surgery is evident; for asymptomatic lesions we only consider a simultaneous procedure in special cases where there exists a threat of neurological complications."}
{"id": "PMID:463485", "title": "Transient liver deterioration induced by general anesthesia.", "content": "The disappearance rate of bromsulphthalein (BSP) has been studied in 43 patients before and during vaginal hysterectomy under three kinds of anesthesia: Halothane anesthesia, neuroleptanalgesia, and peridural anesthesia. Furthermore, serum enzymes were measured before and after surgery. The half-life of BSP in the elimination phase is significantly prolonged during general anesthesia but not during the peridural anesthesia in the same procedure. There were slight increases of gamma-glutamyltranspeptidase in the later postoperative days with no changes of transaminases and alkaline phosphatase. It can be concluded that the known post-operative transient liver dysfunction, detected only by the BSP-test, occurs during the performance of surgery and is caused by the influence of the general anesthesia on the intrahepatic cell metabolism and not by the operative procedure itself.", "contents": "Transient liver deterioration induced by general anesthesia. The disappearance rate of bromsulphthalein (BSP) has been studied in 43 patients before and during vaginal hysterectomy under three kinds of anesthesia: Halothane anesthesia, neuroleptanalgesia, and peridural anesthesia. Furthermore, serum enzymes were measured before and after surgery. The half-life of BSP in the elimination phase is significantly prolonged during general anesthesia but not during the peridural anesthesia in the same procedure. There were slight increases of gamma-glutamyltranspeptidase in the later postoperative days with no changes of transaminases and alkaline phosphatase. It can be concluded that the known post-operative transient liver dysfunction, detected only by the BSP-test, occurs during the performance of surgery and is caused by the influence of the general anesthesia on the intrahepatic cell metabolism and not by the operative procedure itself."}
{"id": "PMID:463486", "title": "Liver injury and lipid metabolism: sex differences in the fatty liver induced by d-galactosamine.", "content": "The time-course of plasma lipid alterations and of triglyceride accumulation in the liver was investigated in male and female rats 12, 24 and 48 hours after treatment with 3.48 mmole/kg (0.75 g/kg) D-galactosamine (Ga1N). In the early stages of Ga1N-induced liver injury the concentrations of triglycerides, phospholipids and total cholesterol decreased, while in the later stages these values in the plasma increased above normal, especially in male animals. In contrast, glucose concentrations continually decreased, while free fatty acid (FFA) levels rose to twice those normal in female animals. Male animals had significantly lower FFA-values throughout the experiment. Consistently, the triglyceride accumulation on liver was 75 mg/g in female animals 24 hours after Ga1N administration, while male animals in the average showed only 33 mg/g triglycerides. Similar fatty infiltrations were obtained in female animals with the rather low doses of 1.16 and 2.32 mmol/kg Ga1N. It is concluded that the increase of FFA-influx after Ga1N administration is the main cause for fatty infiltration, the sex differences in the plasma FFA concentrations explaining the net differences in liver triglyceride accumulation. Additional effects in the pathogenesis of fatty liver might stem from disturbed glycosylation reactions and/or an altered secretion and metabolism of lipoproteins after Ga1N-induced liver injury.", "contents": "Liver injury and lipid metabolism: sex differences in the fatty liver induced by d-galactosamine. The time-course of plasma lipid alterations and of triglyceride accumulation in the liver was investigated in male and female rats 12, 24 and 48 hours after treatment with 3.48 mmole/kg (0.75 g/kg) D-galactosamine (Ga1N). In the early stages of Ga1N-induced liver injury the concentrations of triglycerides, phospholipids and total cholesterol decreased, while in the later stages these values in the plasma increased above normal, especially in male animals. In contrast, glucose concentrations continually decreased, while free fatty acid (FFA) levels rose to twice those normal in female animals. Male animals had significantly lower FFA-values throughout the experiment. Consistently, the triglyceride accumulation on liver was 75 mg/g in female animals 24 hours after Ga1N administration, while male animals in the average showed only 33 mg/g triglycerides. Similar fatty infiltrations were obtained in female animals with the rather low doses of 1.16 and 2.32 mmol/kg Ga1N. It is concluded that the increase of FFA-influx after Ga1N administration is the main cause for fatty infiltration, the sex differences in the plasma FFA concentrations explaining the net differences in liver triglyceride accumulation. Additional effects in the pathogenesis of fatty liver might stem from disturbed glycosylation reactions and/or an altered secretion and metabolism of lipoproteins after Ga1N-induced liver injury."}
{"id": "PMID:463487", "title": "Haem biosynthesis in cutaneous hepatic porphyria: comparison with alcoholism and liver disease.", "content": "The enzymes of haem biosynthesis have been measured in the peripheral blood of 13 patients with cutaneous hepatic porphyria. The activity of leucocyte delta-aminolaevulinic acid synthase was significantly elevated (p less than 0.001) as was that of erythrocyte porphobilinogen deaminase (p less than 0.05). Leucocyte ferrochelatase activity was depressed (p less than 0.001) and the activity of erythrocyte uroporphyrinogen decarboxylase did not significantly differ from control values. Similar enzyme activities were assayed in 12 chronic alcoholics and 8 patients with liver disease and the results differed markedly from those obtained from the porphyric patients. It is unlikely that the raised leucocyte delta-amino-laevulinic acid synthase activity can be attributed to alcohol ingestion or liver disease. A defect in the activity of uroporphyrinogen decarboxylase may exist in cutaneous hepatic porphyria but this could not be demonstrated in erythrocytes in this study.", "contents": "Haem biosynthesis in cutaneous hepatic porphyria: comparison with alcoholism and liver disease. The enzymes of haem biosynthesis have been measured in the peripheral blood of 13 patients with cutaneous hepatic porphyria. The activity of leucocyte delta-aminolaevulinic acid synthase was significantly elevated (p less than 0.001) as was that of erythrocyte porphobilinogen deaminase (p less than 0.05). Leucocyte ferrochelatase activity was depressed (p less than 0.001) and the activity of erythrocyte uroporphyrinogen decarboxylase did not significantly differ from control values. Similar enzyme activities were assayed in 12 chronic alcoholics and 8 patients with liver disease and the results differed markedly from those obtained from the porphyric patients. It is unlikely that the raised leucocyte delta-amino-laevulinic acid synthase activity can be attributed to alcohol ingestion or liver disease. A defect in the activity of uroporphyrinogen decarboxylase may exist in cutaneous hepatic porphyria but this could not be demonstrated in erythrocytes in this study."}
{"id": "PMID:463488", "title": "Effect of glucagon on glycogen content of the dog and rat intestinal mucosa.", "content": "The effect of intraarterial glucagon injection on the glycogen content of the intestinal mucosa of the dog and the rat has been investigated. In both species significant glucagon-induced reduction of mucosal glycogen contents was revealed.", "contents": "Effect of glucagon on glycogen content of the dog and rat intestinal mucosa. The effect of intraarterial glucagon injection on the glycogen content of the intestinal mucosa of the dog and the rat has been investigated. In both species significant glucagon-induced reduction of mucosal glycogen contents was revealed."}
{"id": "PMID:463489", "title": "Functional analysis of organic sphincter systems by elastance-measurement. Evaluation of a new method.", "content": "The function of organic sphincter systems cannot be described by measuring a static pressure, but only by applying a dynamic procedure of measurement that takes into account the characteristics of smooth muscles. Therefore, the elastance-method has been developed. Experiences with this method and its clinical value are demonstrated when one applies this procedure to measurements in the lower oesophagus, the duodenal papilla and the anal canal.", "contents": "Functional analysis of organic sphincter systems by elastance-measurement. Evaluation of a new method. The function of organic sphincter systems cannot be described by measuring a static pressure, but only by applying a dynamic procedure of measurement that takes into account the characteristics of smooth muscles. Therefore, the elastance-method has been developed. Experiences with this method and its clinical value are demonstrated when one applies this procedure to measurements in the lower oesophagus, the duodenal papilla and the anal canal."}
{"id": "PMID:463490", "title": "Interaction of synthetic human big gastrin with blood proteins of man and animals.", "content": "Radio gel and affin chromatography were used to study big gastrin interaction with blood proteins of man and animals. The experiments showed that big gastrin interacts with serum proteins in vitro. The gastrin-blood protein complex is labile and readily dissociates (T 1/2 = 8--14 min). A more stable complex is found in acidic medium. Ceruloplasmin is one of the blood serum proteins able to interact with big gastrin. The stability of hormone-protein complex, formed by gastrin and ceruloplasmin, is dependent upon hormone concentration. With the addition of C-terminal penta--and octapeptides to labelled hormone, binding increased. It is speculated that formation of labile gastrin-blood protein complexes is necessary for selective gastrin transport from hormone-producing cells to target cells.", "contents": "Interaction of synthetic human big gastrin with blood proteins of man and animals. Radio gel and affin chromatography were used to study big gastrin interaction with blood proteins of man and animals. The experiments showed that big gastrin interacts with serum proteins in vitro. The gastrin-blood protein complex is labile and readily dissociates (T 1/2 = 8--14 min). A more stable complex is found in acidic medium. Ceruloplasmin is one of the blood serum proteins able to interact with big gastrin. The stability of hormone-protein complex, formed by gastrin and ceruloplasmin, is dependent upon hormone concentration. With the addition of C-terminal penta--and octapeptides to labelled hormone, binding increased. It is speculated that formation of labile gastrin-blood protein complexes is necessary for selective gastrin transport from hormone-producing cells to target cells."}
{"id": "PMID:463491", "title": "Gastric basal secretion during exercise and restitution in patients with chronic duodenal ulcer.", "content": "Gastric basal secretion was studied at rest, in the course of one hour's exertion and one hour's restitution in 10 patients with chronic duodenal ulcer, aged 21-52. The subject performed exercise on a Monark ergometer under a load that gave constant pulse rate acceleration equal to 50% of the age-related maximum. Gastric juice samples were collected with the subjects sitting, in three successive phase of rest, exercise, and restitution. It was found that during exercise there was an increase in the gastric juice volume, HCl levels in it, basal secretion volume, and overall sodium, potassium, chloride, calcium, and magnesium content, their concentration in the juice having remained constant. In the course of restitution the above values did not differ in any significant way from those obtained at rest.", "contents": "Gastric basal secretion during exercise and restitution in patients with chronic duodenal ulcer. Gastric basal secretion was studied at rest, in the course of one hour's exertion and one hour's restitution in 10 patients with chronic duodenal ulcer, aged 21-52. The subject performed exercise on a Monark ergometer under a load that gave constant pulse rate acceleration equal to 50% of the age-related maximum. Gastric juice samples were collected with the subjects sitting, in three successive phase of rest, exercise, and restitution. It was found that during exercise there was an increase in the gastric juice volume, HCl levels in it, basal secretion volume, and overall sodium, potassium, chloride, calcium, and magnesium content, their concentration in the juice having remained constant. In the course of restitution the above values did not differ in any significant way from those obtained at rest."}
{"id": "PMID:463492", "title": "Five patients with nonalcoholic diabetic cirrhosis.", "content": "Five nonalcoholic diabetic women were clinically and histologically verified as having micronodular cirrhosis. In this series all the patients were over 50 years of age, and showed obesity, hyperglycemia, enlarged liver and mild abnormalities of liver function tests. The histological findings differed from hepatitic cirrhosis. In two patients serial biopsies confirmed development of cirrhosis from centrilobular necrosis.", "contents": "Five patients with nonalcoholic diabetic cirrhosis. Five nonalcoholic diabetic women were clinically and histologically verified as having micronodular cirrhosis. In this series all the patients were over 50 years of age, and showed obesity, hyperglycemia, enlarged liver and mild abnormalities of liver function tests. The histological findings differed from hepatitic cirrhosis. In two patients serial biopsies confirmed development of cirrhosis from centrilobular necrosis."}
{"id": "PMID:463493", "title": "Serum gastrin in portal and peripheral veins after arginine in man.", "content": "Immunoreactive serum gastrin from portal and peripheral veins was determined in five controls and in five cirrhotic patients with indwelling portal catheters in basal conditions, during and after 30 min arginine infusion, in order to assess whether the human liver is involved in gastrin metabolism. A significant difference was found between portal and peripheral gastrin concentrations in controls at 0, 60 and 90 min; no significant differences were found in cirrhotics. Our findings support the hypothesis of an at least partial breakdown of endogenous gastrin in the human liver.", "contents": "Serum gastrin in portal and peripheral veins after arginine in man. Immunoreactive serum gastrin from portal and peripheral veins was determined in five controls and in five cirrhotic patients with indwelling portal catheters in basal conditions, during and after 30 min arginine infusion, in order to assess whether the human liver is involved in gastrin metabolism. A significant difference was found between portal and peripheral gastrin concentrations in controls at 0, 60 and 90 min; no significant differences were found in cirrhotics. Our findings support the hypothesis of an at least partial breakdown of endogenous gastrin in the human liver."}
{"id": "PMID:463496", "title": "The fine structure of stratified ependyma in the ventricular wall of N-ethyl-N-nitrosourea-treated rats.", "content": "Ependymal stratifications, up to 15 cell layers in thickness at the floor of the lateral ventricle of rats treated transplacentally with N-ethyl-N-nitrosourea (ENU), are described. Ultrastructurally the constituent ependymal cells are well-differentiated and show no morphological signs of neoplasia. The possibility that these findings are due to the toxicity or carcinogenicity of ENU is discussed.", "contents": "The fine structure of stratified ependyma in the ventricular wall of N-ethyl-N-nitrosourea-treated rats. Ependymal stratifications, up to 15 cell layers in thickness at the floor of the lateral ventricle of rats treated transplacentally with N-ethyl-N-nitrosourea (ENU), are described. Ultrastructurally the constituent ependymal cells are well-differentiated and show no morphological signs of neoplasia. The possibility that these findings are due to the toxicity or carcinogenicity of ENU is discussed."}
{"id": "PMID:463497", "title": "Spindle-shaped appendages of IIIab-pyramids filled with lipofuscin: a striking pathological change of the senescent human isocortex.", "content": "Brains from 30 mentally normal men, ranging in age from 18--90 years, were studied to assess the occurrence and development of lipofuscin-filled spindle-shaped appendages of pyramidal cells of the isocortex; these can generally be encountered in the senescent brain within layer IIIab. The number of pigmented spindles was determined in a circumscribed part of prefrontal area revealing a close association of this conspicuous pathological alteration with growing age.", "contents": "Spindle-shaped appendages of IIIab-pyramids filled with lipofuscin: a striking pathological change of the senescent human isocortex. Brains from 30 mentally normal men, ranging in age from 18--90 years, were studied to assess the occurrence and development of lipofuscin-filled spindle-shaped appendages of pyramidal cells of the isocortex; these can generally be encountered in the senescent brain within layer IIIab. The number of pigmented spindles was determined in a circumscribed part of prefrontal area revealing a close association of this conspicuous pathological alteration with growing age."}
{"id": "PMID:463498", "title": "Familial centronuclear myopathy: a haploid DNA disease?", "content": "In the same family three boys (ages 8, 11, 14 years), suffering from a slowly progressive weakness of the flexors of the head and distal muscle groups of the extremities, were found to have a myopathy characterized by the presence of central nuclei in type I fibres. Their mother showed no histopathologic signs but a myopathic pattern on electromyography could be demonstrated. Cytophotometric examination of the anterior tibial muscle of these siblings revealed a reduction in DNA content by 50% in the central and subsarcolemmal nuclei (haploid DNA content) when compared with subsarcolemmal nuclei (diploid DNA content) of the same muscle of their mother. Nuclear DNA concentration in fibrocytes did not differ significantly between members of the family. Therefore, it is assumed that the nuclei of muscle fibres are selectively affected by the disease process which is indicated by a haploid DNA content.", "contents": "Familial centronuclear myopathy: a haploid DNA disease? In the same family three boys (ages 8, 11, 14 years), suffering from a slowly progressive weakness of the flexors of the head and distal muscle groups of the extremities, were found to have a myopathy characterized by the presence of central nuclei in type I fibres. Their mother showed no histopathologic signs but a myopathic pattern on electromyography could be demonstrated. Cytophotometric examination of the anterior tibial muscle of these siblings revealed a reduction in DNA content by 50% in the central and subsarcolemmal nuclei (haploid DNA content) when compared with subsarcolemmal nuclei (diploid DNA content) of the same muscle of their mother. Nuclear DNA concentration in fibrocytes did not differ significantly between members of the family. Therefore, it is assumed that the nuclei of muscle fibres are selectively affected by the disease process which is indicated by a haploid DNA content."}
{"id": "PMID:463499", "title": "[Melanosis cerebelli. Ultrastructure and infrared absorption of the pigment (author's transl)].", "content": "The pigment present in melanosis cerebelli where it is deposited in the dentate nucleus and within the cerebellar granular layer is similar to that of S. nigra as judged from the typical infrared absorption bands near wave numbers 3.450 cm-1 and 1.650 cm-1, respectively. According to electron microscopic findings, this type of melanin arises within the astrocytes of the dentate nucleus. The astrocytic melanosomes exhibit a relatively monotonous substructure reaching diameters of 30 mu and more, and they differ in this respect from the triadic neuronal melanosomes of S. nigra that are strongly reminiscent of lipofuscin granules. These differences may be due to additional components of melanosomes, apparently specific of a particular cell type, and they are in turn accounted for by the dissimilar cellular metabolic make-up of nigral cells and astrocytes, respectively. This does not dismiss the possibility, however, that the melanin pigments of either type of melanosomes are the same or closely related to each other as far as their basic chemical composition is concerned. The cause of the melanosis of the dentate nucleus is not known. Possibly involutional tissue changes governed by endocrine factors among others may play an important part.", "contents": "[Melanosis cerebelli. Ultrastructure and infrared absorption of the pigment (author's transl)]. The pigment present in melanosis cerebelli where it is deposited in the dentate nucleus and within the cerebellar granular layer is similar to that of S. nigra as judged from the typical infrared absorption bands near wave numbers 3.450 cm-1 and 1.650 cm-1, respectively. According to electron microscopic findings, this type of melanin arises within the astrocytes of the dentate nucleus. The astrocytic melanosomes exhibit a relatively monotonous substructure reaching diameters of 30 mu and more, and they differ in this respect from the triadic neuronal melanosomes of S. nigra that are strongly reminiscent of lipofuscin granules. These differences may be due to additional components of melanosomes, apparently specific of a particular cell type, and they are in turn accounted for by the dissimilar cellular metabolic make-up of nigral cells and astrocytes, respectively. This does not dismiss the possibility, however, that the melanin pigments of either type of melanosomes are the same or closely related to each other as far as their basic chemical composition is concerned. The cause of the melanosis of the dentate nucleus is not known. Possibly involutional tissue changes governed by endocrine factors among others may play an important part."}
{"id": "PMID:463500", "title": "Late-onset malignant astrocytoma in a case of multiple sclerosis. Clinical, neuropathological, virological, and tissue culture studies.", "content": "An unusual case of concurrent MS and anaplastic astrocytoma is presented. MS was diagnosed in a female patient at the age of 22 years. A left side thalamotomy was performed for relief of severe intention tremor at age 28 and at age 32 she received immunosuppressive therapy for 1 year. At the age of 36 after a severe exacerbation of her symptoms a left side fronto-temporal tumor was diagnosed and a subtotal neurosurgical extirpation was performed. Histopathologically, the tumor was an anaplastic astrocytoma, which was further substantiated by electron microscopy and establishment of a permanent cell line in vitro. The cultured tumor cells were negative for measles virus by immunofluorescence. The relationship between the reactive astrocytes in MS plaques and astrocytic neoplasia is discussed.", "contents": "Late-onset malignant astrocytoma in a case of multiple sclerosis. Clinical, neuropathological, virological, and tissue culture studies. An unusual case of concurrent MS and anaplastic astrocytoma is presented. MS was diagnosed in a female patient at the age of 22 years. A left side thalamotomy was performed for relief of severe intention tremor at age 28 and at age 32 she received immunosuppressive therapy for 1 year. At the age of 36 after a severe exacerbation of her symptoms a left side fronto-temporal tumor was diagnosed and a subtotal neurosurgical extirpation was performed. Histopathologically, the tumor was an anaplastic astrocytoma, which was further substantiated by electron microscopy and establishment of a permanent cell line in vitro. The cultured tumor cells were negative for measles virus by immunofluorescence. The relationship between the reactive astrocytes in MS plaques and astrocytic neoplasia is discussed."}
{"id": "PMID:463501", "title": "Paraganglioma in the cauda equina region.", "content": "Some new ultrastructural observations are documented in paraganglioma in the cauda equina region. These include the presence of nerve fibers in the tumor capsule, clear vesicles and tubulo-vesicular structures in some cytoplasmic processes in the tumor, fenestrated endothelial cells, and numerous tubular bodies in some endothelial cells.", "contents": "Paraganglioma in the cauda equina region. Some new ultrastructural observations are documented in paraganglioma in the cauda equina region. These include the presence of nerve fibers in the tumor capsule, clear vesicles and tubulo-vesicular structures in some cytoplasmic processes in the tumor, fenestrated endothelial cells, and numerous tubular bodies in some endothelial cells."}
{"id": "PMID:463502", "title": "The morphopathologic substrates of concussion?", "content": "Neuronal inundation with i.v. infused horseradish peroxidase was studied following concussive brain injury by means of both light and electron microscopy. In animals sustaining mechanical brain injury of insufficient intensity as to elicit either microscopic intraparenchymal hemorrhage or other neuropathological change, yet of sufficient intensity as to provoke a physiological concussive response, vascular peroxidase exudation concomitant with neuronal peroxidase inundation occurred throughout the raphe and reticular core. Initially such inundated neurons were totally flooded with the tracer and as such appeared reminiscent of cells visualized in Golgi preparations. However, over the course of a 24-h period these peroxidae flooded neurons apparently organized the peroxidase into vesicles and vacuoles which assumed a perinuclear position from where the peroxidase ultimately reached both the nucleus and nucleolus. It was remarkable that these events occurred without any evidence of subcellular alteration. We interpret such initial inundation with this protein tracer, its ultimate reorganization, and its nuclear and nucleolar uptake as being consistent with some form of subtle and transient neuronal perturbation. We speculate that as such this neuronal perturbation may constitute a morphological correlate of the concussive episode.", "contents": "The morphopathologic substrates of concussion? Neuronal inundation with i.v. infused horseradish peroxidase was studied following concussive brain injury by means of both light and electron microscopy. In animals sustaining mechanical brain injury of insufficient intensity as to elicit either microscopic intraparenchymal hemorrhage or other neuropathological change, yet of sufficient intensity as to provoke a physiological concussive response, vascular peroxidase exudation concomitant with neuronal peroxidase inundation occurred throughout the raphe and reticular core. Initially such inundated neurons were totally flooded with the tracer and as such appeared reminiscent of cells visualized in Golgi preparations. However, over the course of a 24-h period these peroxidae flooded neurons apparently organized the peroxidase into vesicles and vacuoles which assumed a perinuclear position from where the peroxidase ultimately reached both the nucleus and nucleolus. It was remarkable that these events occurred without any evidence of subcellular alteration. We interpret such initial inundation with this protein tracer, its ultimate reorganization, and its nuclear and nucleolar uptake as being consistent with some form of subtle and transient neuronal perturbation. We speculate that as such this neuronal perturbation may constitute a morphological correlate of the concussive episode."}
{"id": "PMID:463503", "title": "The stathmokinetic (mitostatic) effect of vincristine and vinblastine on human gliomas.", "content": "Twelve patients with gliomas received 3H-thymidine intraarterially in combination with an i.v. mitostatic agent (Velban: 0.1 mg/kg or 3 mg/M2 of Vincristine) prior to tumor biopsy. The labeling index (LI) of each tumor was compared with the mitotic index divided by the elapsed time from administration of the mitostatic agent to biopsy (MI/t). Most of the malignant gliomas that had an LI of 5-15%, also yielded an MI/t value of 4-5 x 10(-3), whereas tumors with an LI of less than 5% generally showed very slight (0.1 x 10(-3) mitotic accumulation. Use of a mitostatic agent is suggested to differentiate the proliferative activity of various gliomas.", "contents": "The stathmokinetic (mitostatic) effect of vincristine and vinblastine on human gliomas. Twelve patients with gliomas received 3H-thymidine intraarterially in combination with an i.v. mitostatic agent (Velban: 0.1 mg/kg or 3 mg/M2 of Vincristine) prior to tumor biopsy. The labeling index (LI) of each tumor was compared with the mitotic index divided by the elapsed time from administration of the mitostatic agent to biopsy (MI/t). Most of the malignant gliomas that had an LI of 5-15%, also yielded an MI/t value of 4-5 x 10(-3), whereas tumors with an LI of less than 5% generally showed very slight (0.1 x 10(-3) mitotic accumulation. Use of a mitostatic agent is suggested to differentiate the proliferative activity of various gliomas."}
{"id": "PMID:463504", "title": "The perineuronal satellite oligodendrocyte. A role in remyelination.", "content": "Demyelination was induced in the superior cerebellar peduncles of weanling mice by the administration of Cuprizone. Remyelination occurred when the animals were replaced on a normal diet. Perineuronal satellite oligodendrocytes in the periventricular gray were clearly seen to be remyelinating axons. This study demonstrates for the first time the role of these cells in remyelination, and raises the possibility that they may be involved in normal myelination of the central nervous system.", "contents": "The perineuronal satellite oligodendrocyte. A role in remyelination. Demyelination was induced in the superior cerebellar peduncles of weanling mice by the administration of Cuprizone. Remyelination occurred when the animals were replaced on a normal diet. Perineuronal satellite oligodendrocytes in the periventricular gray were clearly seen to be remyelinating axons. This study demonstrates for the first time the role of these cells in remyelination, and raises the possibility that they may be involved in normal myelination of the central nervous system."}
{"id": "PMID:463505", "title": "Electron microscopical demonstration of nucleic acids in virus-like particles in the skeletal muscle of a traffic accident victim.", "content": "Picornavirus-like crystalline arrays were found in the skeletal muscle of a victim of a traffic accident. Individual particles measured approximately 22 nm in diameter and were arrayed in a hexagonal or triangular pattern. Cis-platinum (II) staining of these virus-like particles showed that these particles were not glycogen but contained nucleic acids, and the staining according to Bernhard also showed these particles contained RNA. Through the combination of the methods of Heinen (1977) and Bernhard (1969), we can confirm that virus-like crystalline structures found electron microscopically contain nucleic acids and possibly represent virions.", "contents": "Electron microscopical demonstration of nucleic acids in virus-like particles in the skeletal muscle of a traffic accident victim. Picornavirus-like crystalline arrays were found in the skeletal muscle of a victim of a traffic accident. Individual particles measured approximately 22 nm in diameter and were arrayed in a hexagonal or triangular pattern. Cis-platinum (II) staining of these virus-like particles showed that these particles were not glycogen but contained nucleic acids, and the staining according to Bernhard also showed these particles contained RNA. Through the combination of the methods of Heinen (1977) and Bernhard (1969), we can confirm that virus-like crystalline structures found electron microscopically contain nucleic acids and possibly represent virions."}
{"id": "PMID:463506", "title": "Scanning electron microscopy of in vitro grown cells from experimentally induced neurogenic tumors.", "content": "Serially transplanted neurogenic tumors of experimental origin were explanted in vitro and analyzed by scanning electron microscopy. In explants of intracerebrally transplanted gliomas, small stellate cells with branching processes were observed; in transplanted tumors of the peripheral nervous system, slender bipolar cells as well as fibroblasts emerged. The stereoscopic features were compared with the picture of conventional light microscopy. The findings are consistent with the assumption of the glial derivation of CNS-tumors induced with neurotropic alkylating carcinogens. The peripheral tumors are composed of Schwann-cell like elements and fibroblasts. The significance of the results for the cytogenetic derivation of brain tumors are discussed.", "contents": "Scanning electron microscopy of in vitro grown cells from experimentally induced neurogenic tumors. Serially transplanted neurogenic tumors of experimental origin were explanted in vitro and analyzed by scanning electron microscopy. In explants of intracerebrally transplanted gliomas, small stellate cells with branching processes were observed; in transplanted tumors of the peripheral nervous system, slender bipolar cells as well as fibroblasts emerged. The stereoscopic features were compared with the picture of conventional light microscopy. The findings are consistent with the assumption of the glial derivation of CNS-tumors induced with neurotropic alkylating carcinogens. The peripheral tumors are composed of Schwann-cell like elements and fibroblasts. The significance of the results for the cytogenetic derivation of brain tumors are discussed."}
{"id": "PMID:463507", "title": "A quantitative study of the composition of cerebellar cortical dysplasias.", "content": "The areas of molecular and granular layers in four large cerebellar cortical dysplasias were measured with the planimeter and the Purkinje cells were counted. There was a relative deficit of molecular layer and a still greater deficit of Purkinje cells in three of the four dysplasias. The fourth one had normal numbers of Purkinje cells per molecular layer and a relative deficit of granular layer. These abnormal proportions in the composition of a dysplasia may relate to the timing of its origin.", "contents": "A quantitative study of the composition of cerebellar cortical dysplasias. The areas of molecular and granular layers in four large cerebellar cortical dysplasias were measured with the planimeter and the Purkinje cells were counted. There was a relative deficit of molecular layer and a still greater deficit of Purkinje cells in three of the four dysplasias. The fourth one had normal numbers of Purkinje cells per molecular layer and a relative deficit of granular layer. These abnormal proportions in the composition of a dysplasia may relate to the timing of its origin."}
{"id": "PMID:463508", "title": "Rosenthal fibers formation in the central nervous system. Its relation to Alexander's disease.", "content": "Subpial and subependymal gliosis with abundant Rosenthal fiber (RF) formation are described in two patients. In one, the process appeared as a narrow band over the entire neuraxis and was not accompanied by neurologic manifestations. In the other, the changes were mostly restricted to the lower medulla and upper cervical cord and displayed invasive features. The glial proliferation with RF in the second patient was associated with an inflammatory component, dense collagen deposition, perivascular Schwannosis and demyelination. Involvement of some of the medullary nuclei and their tracts may explain the patient's vague bulbar symptomatology. The underlying cause which led to astrocytic proliferation with excessive RF in these two patients remains elusive. Possible factors contributing to this phenomenon are discussed in the context of Alexander's disease.", "contents": "Rosenthal fibers formation in the central nervous system. Its relation to Alexander's disease. Subpial and subependymal gliosis with abundant Rosenthal fiber (RF) formation are described in two patients. In one, the process appeared as a narrow band over the entire neuraxis and was not accompanied by neurologic manifestations. In the other, the changes were mostly restricted to the lower medulla and upper cervical cord and displayed invasive features. The glial proliferation with RF in the second patient was associated with an inflammatory component, dense collagen deposition, perivascular Schwannosis and demyelination. Involvement of some of the medullary nuclei and their tracts may explain the patient's vague bulbar symptomatology. The underlying cause which led to astrocytic proliferation with excessive RF in these two patients remains elusive. Possible factors contributing to this phenomenon are discussed in the context of Alexander's disease."}
{"id": "PMID:463512", "title": "Decay and recovery of the acoustic stapedius reflex in humans.", "content": "The time course of decay and recovery of ipsilateral and contralateral stapedius reflex responses to 2 000 Hz pure tone stimulation was studied in 10 normal-hearing subjects. Reflex responses were found to follow a closely similar time course with respect to both decay and recovery in simultaneous bilateral recordings. The similarity is compatible with the assumption that decay and recovery originate in the afferent auditory system, and not in the muscles. Recovery was 50% complete 250 ms after the end of the stimulus and most subjects had reached their initial amplitude after 1-3 s. The individual correlation between decay and recovery was negative but weak, which is interpreted as showing that these processes have a tendency to balance each other, but are based partly on different mechanisms. The implications of the present results for diagnosis of disorders of the lower auditory system as well as for the evaluation of the protective role of the stapedius reflex against noise damage are pointed out.", "contents": "Decay and recovery of the acoustic stapedius reflex in humans. The time course of decay and recovery of ipsilateral and contralateral stapedius reflex responses to 2 000 Hz pure tone stimulation was studied in 10 normal-hearing subjects. Reflex responses were found to follow a closely similar time course with respect to both decay and recovery in simultaneous bilateral recordings. The similarity is compatible with the assumption that decay and recovery originate in the afferent auditory system, and not in the muscles. Recovery was 50% complete 250 ms after the end of the stimulus and most subjects had reached their initial amplitude after 1-3 s. The individual correlation between decay and recovery was negative but weak, which is interpreted as showing that these processes have a tendency to balance each other, but are based partly on different mechanisms. The implications of the present results for diagnosis of disorders of the lower auditory system as well as for the evaluation of the protective role of the stapedius reflex against noise damage are pointed out."}
{"id": "PMID:463513", "title": "Behavioral auditory function after transection of crossed olivo-cochlear bundle in the cat. V. Pure-tone intensity discrimination.", "content": "This report describes the cat's ability to discriminate pure-tone intensity differences after transection of the crossed olivo-cochlear bundle at the floor of the fourth ventricle. Cats were trained by behavioral (avoidance) conditioning to detect intensity difference above 75 dB re 0.0002 dyne/cm2 of 10 000 Hz tones, using the repetitive method. Postoperative comparison of data from 5 experimental animals and 5 sham-operated control animals showed minimal differences which reflects merely functional biological variance. Neurohistological examination confirmed the accuracy of the lesion placement.", "contents": "Behavioral auditory function after transection of crossed olivo-cochlear bundle in the cat. V. Pure-tone intensity discrimination. This report describes the cat's ability to discriminate pure-tone intensity differences after transection of the crossed olivo-cochlear bundle at the floor of the fourth ventricle. Cats were trained by behavioral (avoidance) conditioning to detect intensity difference above 75 dB re 0.0002 dyne/cm2 of 10 000 Hz tones, using the repetitive method. Postoperative comparison of data from 5 experimental animals and 5 sham-operated control animals showed minimal differences which reflects merely functional biological variance. Neurohistological examination confirmed the accuracy of the lesion placement."}
{"id": "PMID:463515", "title": "B\u00e9k\u00e9sy audiometry in evaluation of hearing in cases of raised intracranial pressure.", "content": "The paper reports findings in a B\u00e9k\u00e9sy audiometry study on 50 ears of 27 patients with raised intracranial pressure. The B\u00e9k\u00e9sy audiometry was done both preoperatively and after operation when the raised intracranial pressure had been controlled. The study showed a significant hearing loss in B\u00e9k\u00e9sy audiometry due to raised intracranial pressure. However, only low frequencies of 125-250-500 Hz showed a reversal of hearing loss which was statistically significant. The type II pattern of B\u00e9k\u00e9sy audiometry changed to type I after control of the raised intracranial pressure. The type IV and V patterns had a tendency to change to B\u00e9k\u00e9sy I or II, on control of the raised intracranial pressure.", "contents": "B\u00e9k\u00e9sy audiometry in evaluation of hearing in cases of raised intracranial pressure. The paper reports findings in a B\u00e9k\u00e9sy audiometry study on 50 ears of 27 patients with raised intracranial pressure. The B\u00e9k\u00e9sy audiometry was done both preoperatively and after operation when the raised intracranial pressure had been controlled. The study showed a significant hearing loss in B\u00e9k\u00e9sy audiometry due to raised intracranial pressure. However, only low frequencies of 125-250-500 Hz showed a reversal of hearing loss which was statistically significant. The type II pattern of B\u00e9k\u00e9sy audiometry changed to type I after control of the raised intracranial pressure. The type IV and V patterns had a tendency to change to B\u00e9k\u00e9sy I or II, on control of the raised intracranial pressure."}
{"id": "PMID:463516", "title": "Simulation of low-pass hearing loss in a Finnish speech discrimination test.", "content": "Three different degrees of clinical hearing loss of low-pass type were stimulated by a filter apparatus. The equipment to simulate a given hearing loss is described. The speech discrimination curves corresponding to the normal hearing and to the three degrees of hearing loss were measured in normal hearing adults using Finnish PB-words. The stimulation of a light hearing loss of low-pass type yielded a two-staged discrimination curve. The discrimination curves of a moderate and a severe hearing loss crossed at a very high output level. The cause of this phenomenon is discussed on the ground of the exceptional vowel/consonant relation and intensity/frequency distribution in the Finnish language.", "contents": "Simulation of low-pass hearing loss in a Finnish speech discrimination test. Three different degrees of clinical hearing loss of low-pass type were stimulated by a filter apparatus. The equipment to simulate a given hearing loss is described. The speech discrimination curves corresponding to the normal hearing and to the three degrees of hearing loss were measured in normal hearing adults using Finnish PB-words. The stimulation of a light hearing loss of low-pass type yielded a two-staged discrimination curve. The discrimination curves of a moderate and a severe hearing loss crossed at a very high output level. The cause of this phenomenon is discussed on the ground of the exceptional vowel/consonant relation and intensity/frequency distribution in the Finnish language."}
{"id": "PMID:463514", "title": "A developmental study of the sound pressure transformation by the head of the cat.", "content": "The interaural intensity difference (IID) created by the imposition of a cat's body in a free sound field was measured in two age groups of kittens and one group of adult cats. For all animals significantly larger IIDs were obtained for high than for low frequency stimulation. The frequency at which peak IID occurred was higher for the younger group of kittens than for either the Old Kittens or the Adults. Above 4 kHz the Adults showed a marked variation of IID with azimuth, whilst the Young Kittens showed little variation of IID with azimuth at frequencies below 10 kHz. It was found that Adult-like IIDs are achieved before physical maturation is complete. The results are discussed with reference to the physiological development of sensory systems and the implications for a possible post-natal plasticity of the auditory system are examined.", "contents": "A developmental study of the sound pressure transformation by the head of the cat. The interaural intensity difference (IID) created by the imposition of a cat's body in a free sound field was measured in two age groups of kittens and one group of adult cats. For all animals significantly larger IIDs were obtained for high than for low frequency stimulation. The frequency at which peak IID occurred was higher for the younger group of kittens than for either the Old Kittens or the Adults. Above 4 kHz the Adults showed a marked variation of IID with azimuth, whilst the Young Kittens showed little variation of IID with azimuth at frequencies below 10 kHz. It was found that Adult-like IIDs are achieved before physical maturation is complete. The results are discussed with reference to the physiological development of sensory systems and the implications for a possible post-natal plasticity of the auditory system are examined."}
{"id": "PMID:463519", "title": "Population differences in cellularity of the mastoid process.", "content": "Adult crania from four human skeletal population samples were radiographed in order to study variation of the air cell system in the mastoid process. Six types of processes were recognized, i.e., sclerotic, diploic, diplo-pneumatic, pneumatic, and highly pneumatic. Frequency of non-pneumatized (acellular) processes in Eskimos was significantly lower than that found in the other three populations. Female crania in each sample showed fewer acellular processes than male, but the differences were statistically significant only in the Whites, Eskimos, and total sex samples. While present in each population, frequency of asymmetry of type was significant in only Whites and Eskimos. These results provide some additional evidence that a genetic factor is operant in determining the degree of cellularity of mastoid processes.", "contents": "Population differences in cellularity of the mastoid process. Adult crania from four human skeletal population samples were radiographed in order to study variation of the air cell system in the mastoid process. Six types of processes were recognized, i.e., sclerotic, diploic, diplo-pneumatic, pneumatic, and highly pneumatic. Frequency of non-pneumatized (acellular) processes in Eskimos was significantly lower than that found in the other three populations. Female crania in each sample showed fewer acellular processes than male, but the differences were statistically significant only in the Whites, Eskimos, and total sex samples. While present in each population, frequency of asymmetry of type was significant in only Whites and Eskimos. These results provide some additional evidence that a genetic factor is operant in determining the degree of cellularity of mastoid processes."}
{"id": "PMID:463517", "title": "Growth influence on tubal function.", "content": "The influence of growth on tubal function was studied in 26 children by means of tubal function tests in a pressure chamber and by geometric analysis of lateral cephalograms. Good tubal function coincided with mesoor brachycephalic skeletons, poor tubal function with dolichocephalic morphologic patterns. According to our results, tubal function seems to be strongly influenced by the growth processes of the skull.", "contents": "Growth influence on tubal function. The influence of growth on tubal function was studied in 26 children by means of tubal function tests in a pressure chamber and by geometric analysis of lateral cephalograms. Good tubal function coincided with mesoor brachycephalic skeletons, poor tubal function with dolichocephalic morphologic patterns. According to our results, tubal function seems to be strongly influenced by the growth processes of the skull."}
{"id": "PMID:463521", "title": "Loss of visual suppression of caloric nystagmus in cats.", "content": "Loss of visual suppression (VS) of caloric nystagmus was produced after creating flocculus lesions. The flocculus receives visual signals through a climbing fiber pathway via the inferior olive (IO) and through a mossy fiber pathway (MF) presumably via the superior colliculus (SC). In order to elucidate the prefloccular nuclei responsible for VS of caloric nystagmus, VS of caloric nystagmus was investigated after making lesions in such nuclei as the SC and the IO in 42 cats. After the IO lesion, VS of caloric nystagmus was revealed in all IO-lesioned cats throughout the whole experimental course. After the SC lesion, loss of VS was constantly observed and persisted in 7 out of 9 cats. Hence, the MF pathway via the SC is believed to be the most likely candidate for the immediate modification of the vestibuloocular reflex by visual stimuli.", "contents": "Loss of visual suppression of caloric nystagmus in cats. Loss of visual suppression (VS) of caloric nystagmus was produced after creating flocculus lesions. The flocculus receives visual signals through a climbing fiber pathway via the inferior olive (IO) and through a mossy fiber pathway (MF) presumably via the superior colliculus (SC). In order to elucidate the prefloccular nuclei responsible for VS of caloric nystagmus, VS of caloric nystagmus was investigated after making lesions in such nuclei as the SC and the IO in 42 cats. After the IO lesion, VS of caloric nystagmus was revealed in all IO-lesioned cats throughout the whole experimental course. After the SC lesion, loss of VS was constantly observed and persisted in 7 out of 9 cats. Hence, the MF pathway via the SC is believed to be the most likely candidate for the immediate modification of the vestibuloocular reflex by visual stimuli."}
{"id": "PMID:463518", "title": "Peroperative drill-generated noise levels in ear surgery.", "content": "A simple technique for the measuring of sound intensities produced by drilling in ear surgery is presented. In 6 patients the sound level from drilling in the mastoid exceeded 100 dBA. The drilling device should be so designed as to avoid acoustic trauma. Furthermore, a surgical technique should be selected, which minimizes the noise levels and duration of exposure as far as is possible.", "contents": "Peroperative drill-generated noise levels in ear surgery. A simple technique for the measuring of sound intensities produced by drilling in ear surgery is presented. In 6 patients the sound level from drilling in the mastoid exceeded 100 dBA. The drilling device should be so designed as to avoid acoustic trauma. Furthermore, a surgical technique should be selected, which minimizes the noise levels and duration of exposure as far as is possible."}
{"id": "PMID:463523", "title": "Cupula motion in the semicircular canal of the skate, Raja erinacea. An experimental investigation.", "content": "An upper bound on the magnitude of semicurcular canal cupula motion was experimentally determined in the isolated labyrinth of the skate, Raja erinacea. To visualize the cupula, a glass pipette was pushed through the ampullary wall, and local regions of the cupula were stained by slow infusion of small amounts of Alcian Blue dye. Caloric stimuli which produced large changes in single unit activity in the ampullary nerve and which often recruited several larger, previously silent units were found to produce on detectable cupula motion as seen through the ampulla wall. However when the cupula was first grossly displaced, motion was thereafter observed in response to identical caloric stimuli. Analysis of afferent responses indicates that the normal range of cupula motion in the skate is below the optical resolution of the method, conservatively estimated as 3--5 micrometers.", "contents": "Cupula motion in the semicircular canal of the skate, Raja erinacea. An experimental investigation. An upper bound on the magnitude of semicurcular canal cupula motion was experimentally determined in the isolated labyrinth of the skate, Raja erinacea. To visualize the cupula, a glass pipette was pushed through the ampullary wall, and local regions of the cupula were stained by slow infusion of small amounts of Alcian Blue dye. Caloric stimuli which produced large changes in single unit activity in the ampullary nerve and which often recruited several larger, previously silent units were found to produce on detectable cupula motion as seen through the ampulla wall. However when the cupula was first grossly displaced, motion was thereafter observed in response to identical caloric stimuli. Analysis of afferent responses indicates that the normal range of cupula motion in the skate is below the optical resolution of the method, conservatively estimated as 3--5 micrometers."}
{"id": "PMID:463522", "title": "Some observations on negative endocochlear potential during anoxia.", "content": "The effects of anoxia on the endocochlear potential (EP) and +K and +Na concentrations in the endolymph were studied in three groups of guinea pigs: kanamycin-treated guinea pigs, waltzing guinea pigs and normal guinea pigs. The magnitude of the EP in kanamycin-treated guinea pigs and waltzing guinea pigs did not show marked deviation from that observed in normal animals. The +K and +Na concentrations in the endolymph in those animals with severe suppression of sound-evoked cochlear potentials were also within the normal range. The changes in +K and +Na concentrations in the endolymph in anoxic condition were similar in the three groups of animals. However, the rate of decline of the EP was slower in kanamycin-treated guinea pigs and old waltzing guinea pigs. In young waltzing guinea pigs showing moderate suppression on the cochlear microphonics, the decline of the EP during anoxia was comparable to that observed in normal guinea pigs. The results indicate that anoxia decreases +K and increases +Na concentrations in the endolymph in a similar fashion in kanamycin-treated guinea pigs, waltzing guinea pigs and normal guinea pigs. It is suggested that the decline of the EP during anoxia is correlated with the +K conductance of the organ of Corti.", "contents": "Some observations on negative endocochlear potential during anoxia. The effects of anoxia on the endocochlear potential (EP) and +K and +Na concentrations in the endolymph were studied in three groups of guinea pigs: kanamycin-treated guinea pigs, waltzing guinea pigs and normal guinea pigs. The magnitude of the EP in kanamycin-treated guinea pigs and waltzing guinea pigs did not show marked deviation from that observed in normal animals. The +K and +Na concentrations in the endolymph in those animals with severe suppression of sound-evoked cochlear potentials were also within the normal range. The changes in +K and +Na concentrations in the endolymph in anoxic condition were similar in the three groups of animals. However, the rate of decline of the EP was slower in kanamycin-treated guinea pigs and old waltzing guinea pigs. In young waltzing guinea pigs showing moderate suppression on the cochlear microphonics, the decline of the EP during anoxia was comparable to that observed in normal guinea pigs. The results indicate that anoxia decreases +K and increases +Na concentrations in the endolymph in a similar fashion in kanamycin-treated guinea pigs, waltzing guinea pigs and normal guinea pigs. It is suggested that the decline of the EP during anoxia is correlated with the +K conductance of the organ of Corti."}
{"id": "PMID:463520", "title": "Following eye movements on the absence of central vision.", "content": "Following responses to constant velocity and sinusoidally oscillating movements of the whole visual environment were examined in normal subjects wearing blinkers to obscure central vision or subjected to a photo flash to induce a central scotoma, in patients with central scotomas of pathological origin and in one patient with a central scotoma in an immobile eye which provided open loop testing. Good following and brisk nystagmus were produced in patients with central scotomas and subjects with flash scotomas; it was subjectively evident that the scotoma itself could be used as a target to generate open loop pursuit and augment peripherally induced following responses. Following responses in subjects with blinkers were weak, possibly reflecting that, in everyday life, eye movements induced by movements of the visual background have to be suppressed. Open loop responses were strong, suggesting that the periphery has the latent potential to mediate good following. The findings provide a unified explanation for the various patterns of optokinetic nystagmus.", "contents": "Following eye movements on the absence of central vision. Following responses to constant velocity and sinusoidally oscillating movements of the whole visual environment were examined in normal subjects wearing blinkers to obscure central vision or subjected to a photo flash to induce a central scotoma, in patients with central scotomas of pathological origin and in one patient with a central scotoma in an immobile eye which provided open loop testing. Good following and brisk nystagmus were produced in patients with central scotomas and subjects with flash scotomas; it was subjectively evident that the scotoma itself could be used as a target to generate open loop pursuit and augment peripherally induced following responses. Following responses in subjects with blinkers were weak, possibly reflecting that, in everyday life, eye movements induced by movements of the visual background have to be suppressed. Open loop responses were strong, suggesting that the periphery has the latent potential to mediate good following. The findings provide a unified explanation for the various patterns of optokinetic nystagmus."}
{"id": "PMID:463524", "title": "The immunological status of allografts in the middle ear.", "content": "In a series of experiments using two inbred rat strains differing in respect of their major histocompatibility antigens, and a sensitive test for antibody directed against major histocompatibility antigens, it was shown that (i) grafts known to be highly immunogenic (10(7)) lymphocytes) regularly immunized their hosts when injected intratympanically; the immune response could not be ascribed to leakage of cells into either the external auditory meatus or the nasopharynx, (ii) immunization occurred at least partly via the regional lymphatics, (iii) single orthotopic or heterotopic incus grafts immunized their hosts sporadically, and (iv) the variable immunization which followed single ossicular grafts was due to their relatively low content of antigen.", "contents": "The immunological status of allografts in the middle ear. In a series of experiments using two inbred rat strains differing in respect of their major histocompatibility antigens, and a sensitive test for antibody directed against major histocompatibility antigens, it was shown that (i) grafts known to be highly immunogenic (10(7)) lymphocytes) regularly immunized their hosts when injected intratympanically; the immune response could not be ascribed to leakage of cells into either the external auditory meatus or the nasopharynx, (ii) immunization occurred at least partly via the regional lymphatics, (iii) single orthotopic or heterotopic incus grafts immunized their hosts sporadically, and (iv) the variable immunization which followed single ossicular grafts was due to their relatively low content of antigen."}
{"id": "PMID:463525", "title": "Response of nasal airway resistance to hypercapnia and hypoxia in the dog.", "content": "Nasal airway resistance was calculated in 9 anesthetized mongrel dogs by measuring the pressure difference across the nasal airway while a continuous flow of humidified air was passed through the upper airway. Hypercapnia produced a significant decrease in nasal airway resistance (P less than 0.05, Wilcoxon signed-rank test), which was proportional to the Paco2 over the range of 40 to 70 torr. Hypoxia produced a decrease in nasal airway resistance only when there was severe depression of Pao2. Direct stimulation of peripheral chemoreceptors by intravenously administered NaCN (100 microgram/kg) resulted in a significant decrease in nasal airway resistance (P = 0.06, Wilcoxon signed-rank test). Interruption of the cervical sympathetic trunk bilaterally abolished or reversed the response of the nasal airway resistance to hypercapnia and to NaCN. The study indicates that the nasal airway can modify airway resistance in response to respiratory stimuli and that this reflex is mediated by the sympathetic nervous system.", "contents": "Response of nasal airway resistance to hypercapnia and hypoxia in the dog. Nasal airway resistance was calculated in 9 anesthetized mongrel dogs by measuring the pressure difference across the nasal airway while a continuous flow of humidified air was passed through the upper airway. Hypercapnia produced a significant decrease in nasal airway resistance (P less than 0.05, Wilcoxon signed-rank test), which was proportional to the Paco2 over the range of 40 to 70 torr. Hypoxia produced a decrease in nasal airway resistance only when there was severe depression of Pao2. Direct stimulation of peripheral chemoreceptors by intravenously administered NaCN (100 microgram/kg) resulted in a significant decrease in nasal airway resistance (P = 0.06, Wilcoxon signed-rank test). Interruption of the cervical sympathetic trunk bilaterally abolished or reversed the response of the nasal airway resistance to hypercapnia and to NaCN. The study indicates that the nasal airway can modify airway resistance in response to respiratory stimuli and that this reflex is mediated by the sympathetic nervous system."}
{"id": "PMID:463530", "title": "The protective effect of acute phase reactants in neonatal sepsis.", "content": "Phase reactants were evaluated prospectively in babies suspected of having sepsis. Among 318 babies, there were 22 proven and 10 \"very probable\" cases of neonatal sepsis. Of the proven cases 14 survived and 8 died. The survivors had a positive latex C-reactive protein (CRP) in 11 cases and an alpha1-acid glycoprotein (AGP) level greater than 0.5 g/l in 12 cases. Among those who died, one had a positive latex CRP and none had AGP greater than 0.5 g/l. These findings were supported by positive CRP and elevated AGP in almost all \"very probable\" cases, all of whom survived. These data in newborn infants support the hypothesis that acute phase reactants have a functional role in combating infection.", "contents": "The protective effect of acute phase reactants in neonatal sepsis. Phase reactants were evaluated prospectively in babies suspected of having sepsis. Among 318 babies, there were 22 proven and 10 \"very probable\" cases of neonatal sepsis. Of the proven cases 14 survived and 8 died. The survivors had a positive latex C-reactive protein (CRP) in 11 cases and an alpha1-acid glycoprotein (AGP) level greater than 0.5 g/l in 12 cases. Among those who died, one had a positive latex CRP and none had AGP greater than 0.5 g/l. These findings were supported by positive CRP and elevated AGP in almost all \"very probable\" cases, all of whom survived. These data in newborn infants support the hypothesis that acute phase reactants have a functional role in combating infection."}
{"id": "PMID:463532", "title": "Suppressed lymphocyte mitogen-responsiveness in urinary tract infections of children and its correlation to pyelonephritis.", "content": "Cell-mediated immunity (CMI) was studied in a group of 48 children with urinary tract infections (UTI) using a whole blood micromethod for lymphocyte stimulation in vitro. The patients were subdivided into pyelonephritis group (27 cases) and lower urinary tract infection (LUTI) group (21 cases) on the basis of fever, erythrocyte sedimentation rate, C-reative protein and renal concentration capacity. At the acute stage of infection the lymphocyte responsiveness to leucoagglutinin (LA) and concanavalin A (Con A) was suppressed in both groups, but the suppression was much greater in those with pyelonephritis. By 6 weeks after infection the lymphocyte responses were normal in most but not all cases. We conclude that an acute pyelonephritis is associated with marked suppression of CMI and that the latter can be used as an additional criterion for establishing the level of infection. Patients with UTI did not generally appear to have any primary defect of CMI but when suppression of CMI was present, it seemed secondary to an ongoing infection.", "contents": "Suppressed lymphocyte mitogen-responsiveness in urinary tract infections of children and its correlation to pyelonephritis. Cell-mediated immunity (CMI) was studied in a group of 48 children with urinary tract infections (UTI) using a whole blood micromethod for lymphocyte stimulation in vitro. The patients were subdivided into pyelonephritis group (27 cases) and lower urinary tract infection (LUTI) group (21 cases) on the basis of fever, erythrocyte sedimentation rate, C-reative protein and renal concentration capacity. At the acute stage of infection the lymphocyte responsiveness to leucoagglutinin (LA) and concanavalin A (Con A) was suppressed in both groups, but the suppression was much greater in those with pyelonephritis. By 6 weeks after infection the lymphocyte responses were normal in most but not all cases. We conclude that an acute pyelonephritis is associated with marked suppression of CMI and that the latter can be used as an additional criterion for establishing the level of infection. Patients with UTI did not generally appear to have any primary defect of CMI but when suppression of CMI was present, it seemed secondary to an ongoing infection."}
{"id": "PMID:463527", "title": "Biochemical characterization of the proliferation pool of lymphatic cells in the human tonsil and demonstration of the age-dependent pool size reduction.", "content": "The DNA and RNA content and also the thymidine and uridine incorporation kinetics were determined in peripheral lymphocytes and lymphatic cells of the tonsil. The existence of a proliferation pool of lymphoid cells in the tonsil could be established by the comparison of all data. An age-conditioned reduction of the pool size was assumed from the uridine incorporation rate which takes place faster than the organo-specific cell decrease likewise conditioned by age in the tonsil. The abscess tonsil could be explained as a pathological state with considerable reduction of proliferation.", "contents": "Biochemical characterization of the proliferation pool of lymphatic cells in the human tonsil and demonstration of the age-dependent pool size reduction. The DNA and RNA content and also the thymidine and uridine incorporation kinetics were determined in peripheral lymphocytes and lymphatic cells of the tonsil. The existence of a proliferation pool of lymphoid cells in the tonsil could be established by the comparison of all data. An age-conditioned reduction of the pool size was assumed from the uridine incorporation rate which takes place faster than the organo-specific cell decrease likewise conditioned by age in the tonsil. The abscess tonsil could be explained as a pathological state with considerable reduction of proliferation."}
{"id": "PMID:463526", "title": "Biomechanics of the human epiglottis.", "content": "The mechanism that folds the epiglottis down over the closed larynx in the course of swallowing has been unclear. Measurements of the force needed to fold the epiglottis in cadaver specimens exceed the estimated force available from the aryepiglottic muscle. Frame-by-frame analysis of cinefluorograms reveals that deglutitional epiglottic downfolding occurs at the time of maximal elongation of the hyoepiglottic ligament. The observations lead us to propose a conical model of epiglottic downfolding which also explains the conical shape of the epiglottis usual in early infancy. The infant shape may be part of a protective partially closed entrance adaptive to suckling, while maturational widening of the opening adapts to the growing respiratory demands of increasing physical exertion.", "contents": "Biomechanics of the human epiglottis. The mechanism that folds the epiglottis down over the closed larynx in the course of swallowing has been unclear. Measurements of the force needed to fold the epiglottis in cadaver specimens exceed the estimated force available from the aryepiglottic muscle. Frame-by-frame analysis of cinefluorograms reveals that deglutitional epiglottic downfolding occurs at the time of maximal elongation of the hyoepiglottic ligament. The observations lead us to propose a conical model of epiglottic downfolding which also explains the conical shape of the epiglottis usual in early infancy. The infant shape may be part of a protective partially closed entrance adaptive to suckling, while maturational widening of the opening adapts to the growing respiratory demands of increasing physical exertion."}
{"id": "PMID:463533", "title": "Clinical findings and intestinal immunoglobulins in children with partial IgA deficiency.", "content": "We studied the intestinal morphology, and the jejunal and rectal immunoglobulins of 16 children with partial IgA deficiency, defined as serum IgA concentration more than two standard deviations below the mean for age, but higher than the lower limit of sensitivity of single radial immunodiffusion (0.02 g/l). Five of the patients had been treated with phenytoin, 2 had juvenile rheumatoid arthritis, 2 had ulcerative colitis and 5 had recurrent upper respiratory tract infections. The jejunal morphology was normal in every case. In 6 cases normalization of serum IgA occurred during the follow-up, while in one patient with ulcerative colitis the concentration fell below 0.02 g/l. In patients with recurrent infections, there was a decreased frequency of infections when the level of serum igA increased. In 4 patients, IgM-containing cells prodominated in both the jejunal and rectal mucosa, and IgM was increased in the intestinal juice. In 6 patients a significant increase in IgM-containing cells or a decrease in IgA-containing cells or both were seen in either the rectal or jejunal mucosa. There was no correlation between the number of IgA-containing cells in the intestinal mucosa and the serum level of IgA.", "contents": "Clinical findings and intestinal immunoglobulins in children with partial IgA deficiency. We studied the intestinal morphology, and the jejunal and rectal immunoglobulins of 16 children with partial IgA deficiency, defined as serum IgA concentration more than two standard deviations below the mean for age, but higher than the lower limit of sensitivity of single radial immunodiffusion (0.02 g/l). Five of the patients had been treated with phenytoin, 2 had juvenile rheumatoid arthritis, 2 had ulcerative colitis and 5 had recurrent upper respiratory tract infections. The jejunal morphology was normal in every case. In 6 cases normalization of serum IgA occurred during the follow-up, while in one patient with ulcerative colitis the concentration fell below 0.02 g/l. In patients with recurrent infections, there was a decreased frequency of infections when the level of serum igA increased. In 4 patients, IgM-containing cells prodominated in both the jejunal and rectal mucosa, and IgM was increased in the intestinal juice. In 6 patients a significant increase in IgM-containing cells or a decrease in IgA-containing cells or both were seen in either the rectal or jejunal mucosa. There was no correlation between the number of IgA-containing cells in the intestinal mucosa and the serum level of IgA."}
{"id": "PMID:463528", "title": "The effect of ozone and photochemical oxidants on the interferon production by tonsillar lymphocytes.", "content": "In order to elucidate the biological effects of photochemical smog on the tonsil, lymphocytes from human tonsil were exposed to ozone and rabbits were exposed to ozone or photochemical oxidants. The tonsillar lymphocytes were studied for interferon production by Newcastle disease virus and blastoid transformation by PHA. Both interferon production by and blastoid transformation of the human tonsillar lymphocytes decreased markedly on exposure to ozone. Tonsillar lymphocytes from rabbits exposed to photochemical oxidants showed a significant decrease in interferon production. The decrease in interferon production in tonsillar lymphocytes from the rabbit exposed to photochemical oxidants was greater in magnitude than the decrease in interferon production in tonsillar lymphocytes exposed to ozone or non-irradiated automobile exhaust gas. The difference in blastoid transformation between the exposed groups and controls was not significnat. The results suggest that exposure to photochemical oxidants causes some functional changes in tonsillar lymphocytes.", "contents": "The effect of ozone and photochemical oxidants on the interferon production by tonsillar lymphocytes. In order to elucidate the biological effects of photochemical smog on the tonsil, lymphocytes from human tonsil were exposed to ozone and rabbits were exposed to ozone or photochemical oxidants. The tonsillar lymphocytes were studied for interferon production by Newcastle disease virus and blastoid transformation by PHA. Both interferon production by and blastoid transformation of the human tonsillar lymphocytes decreased markedly on exposure to ozone. Tonsillar lymphocytes from rabbits exposed to photochemical oxidants showed a significant decrease in interferon production. The decrease in interferon production in tonsillar lymphocytes from the rabbit exposed to photochemical oxidants was greater in magnitude than the decrease in interferon production in tonsillar lymphocytes exposed to ozone or non-irradiated automobile exhaust gas. The difference in blastoid transformation between the exposed groups and controls was not significnat. The results suggest that exposure to photochemical oxidants causes some functional changes in tonsillar lymphocytes."}
{"id": "PMID:463534", "title": "A prospective study of individual courses of breast feeding.", "content": "A prospective study of the course of breast feeding was carried out in 75 randomly selected women. Weekly interviews were performed from the day after delivery until the termination of breast feeding, but in no case for longer than 6 months. In each case a detailed analysis was made of the factors leading to transient lactation crises or to complete cessation of breast feeding. A second group of 71 mothers, also randomly selected, was interviewed in retrospect only, 6 months after delivery, and served as controls. Twenty-four weeks after delivery 47% of the mothers in the weekly interview group were still breast feeding. The corresponding figure in the control group was 38%. In both groups only few mothers terminated lactation for medical reasons, while about one fourth stopped for some other reason and about one half because of a combination of factors. Brief case reports are presented to illustrate how varying the factors were that threatened breast feeding.", "contents": "A prospective study of individual courses of breast feeding. A prospective study of the course of breast feeding was carried out in 75 randomly selected women. Weekly interviews were performed from the day after delivery until the termination of breast feeding, but in no case for longer than 6 months. In each case a detailed analysis was made of the factors leading to transient lactation crises or to complete cessation of breast feeding. A second group of 71 mothers, also randomly selected, was interviewed in retrospect only, 6 months after delivery, and served as controls. Twenty-four weeks after delivery 47% of the mothers in the weekly interview group were still breast feeding. The corresponding figure in the control group was 38%. In both groups only few mothers terminated lactation for medical reasons, while about one fourth stopped for some other reason and about one half because of a combination of factors. Brief case reports are presented to illustrate how varying the factors were that threatened breast feeding."}
{"id": "PMID:463536", "title": "Smoking during pregnancy--effects on the fetus and on thiocyanate levels in mother and baby.", "content": "Twenty-eight mothers smoking 10--20 cigarettes daily during pregnancy had significantly higher serum thiocyanate concentrations at delivery compared in 25 non-smoking controls. The thiocyanate levels were positively correlated to cigarette consumption and inversely correlated to the birth weights of the infants. A highly significant correlation existed between serum thiocyanate levels of the mother and umbilical cord serum thiocyanate levels, reflecting a nearly complete equilibration. The thiocyanate concentrations in human milk on the 4th day after delivery were considerably lower than the serum concentrations, and no correlation existed between serum and milk concentrations. The infants of smoking mothers had significantly decreased weight and length at birth compared in infants of non-smokers. Birth weights were 3 344 +/- 434 g and 3 620 +/- 504 g respectively (p less than 0.05), and lengths 49.8 +/- 1.7 cm and 51 +/- 1.6 cm respectively (p less than 0.05). No differences were found between smokers and non-smokers in placental and umbilical cord histology, and umbilical cord artery medial area. It is concluded that serum thiocyanate concentration in smokers may be used as an objective measure for smoke exposure, and that maternal cigarette smoking acts as an exogenous factor which interferes with intrauterine development of the fetus in a dose related way.", "contents": "Smoking during pregnancy--effects on the fetus and on thiocyanate levels in mother and baby. Twenty-eight mothers smoking 10--20 cigarettes daily during pregnancy had significantly higher serum thiocyanate concentrations at delivery compared in 25 non-smoking controls. The thiocyanate levels were positively correlated to cigarette consumption and inversely correlated to the birth weights of the infants. A highly significant correlation existed between serum thiocyanate levels of the mother and umbilical cord serum thiocyanate levels, reflecting a nearly complete equilibration. The thiocyanate concentrations in human milk on the 4th day after delivery were considerably lower than the serum concentrations, and no correlation existed between serum and milk concentrations. The infants of smoking mothers had significantly decreased weight and length at birth compared in infants of non-smokers. Birth weights were 3 344 +/- 434 g and 3 620 +/- 504 g respectively (p less than 0.05), and lengths 49.8 +/- 1.7 cm and 51 +/- 1.6 cm respectively (p less than 0.05). No differences were found between smokers and non-smokers in placental and umbilical cord histology, and umbilical cord artery medial area. It is concluded that serum thiocyanate concentration in smokers may be used as an objective measure for smoke exposure, and that maternal cigarette smoking acts as an exogenous factor which interferes with intrauterine development of the fetus in a dose related way."}
{"id": "PMID:463537", "title": "Spontaneous labour and elective induction--a prospective randomized study. Behavioural assessment and neurological examination in the newborn period.", "content": "The effect of induction of labour on the foetus and the newborn was investigated in a prospective randomized study. 41 neonates were studied after induction at full term by amniotomy and intravenous oxytocin infusion. The infusion rate was regulated by the intraamniotic pressure using the Cardiff infusion pump system. 39 neonates served as controls where the labour started spontaneously followed by amniotomy. In both groups foetal heart rate monitoring intraamniotic pressure recordings were performed. There were no differences in Apgar score and pH in cord blood between the groups. The newborns were evaluated the first and the fifth day of life with the Brazelton Neonatal Behavioral Assessment Scale and with a modified Prechtl neurological examination. There were no differences in behaviour and neurological state between the two groups. However, within the groups there were significant differences between the first and the fifth day concerning both neonatal behaviour and neurological state.", "contents": "Spontaneous labour and elective induction--a prospective randomized study. Behavioural assessment and neurological examination in the newborn period. The effect of induction of labour on the foetus and the newborn was investigated in a prospective randomized study. 41 neonates were studied after induction at full term by amniotomy and intravenous oxytocin infusion. The infusion rate was regulated by the intraamniotic pressure using the Cardiff infusion pump system. 39 neonates served as controls where the labour started spontaneously followed by amniotomy. In both groups foetal heart rate monitoring intraamniotic pressure recordings were performed. There were no differences in Apgar score and pH in cord blood between the groups. The newborns were evaluated the first and the fifth day of life with the Brazelton Neonatal Behavioral Assessment Scale and with a modified Prechtl neurological examination. There were no differences in behaviour and neurological state between the two groups. However, within the groups there were significant differences between the first and the fifth day concerning both neonatal behaviour and neurological state."}
{"id": "PMID:463538", "title": "Effect of intrauterine nutritional deprivation on neuromotor behaviour of the newborn.", "content": "The neurological maturation in 25 newborn babies born to severely undernourished mothers was studied by evaluating muscle tone and excitability status. These mothers had weight below the 25th percentile expected for height, haemoglobin less than 80.0 g/l and serum albumin less than 25.0 g/l. Twenty-three babies born to healthy mothers were studied as control. The babies of undernourished mothers demonstrated gross intrauterine growth retardation. There occurred parallel reduction in placental weight and its protein content. The neuromotor behaviour of these newborns showed significant alteration in the performance of most reflexes, resembling normal motor behaviour of preterm infants. As many as 72% newborns could be classified as hypotonic and 56% hypoexcitable. However, no newborn demonstrated hypertonia or hyperexcitability. The parameters of neuromotor assessment were found to have no correlation with the birth weight in both undernourished as well as control group. These observations suggest that muscle tone and excitability are better indices of maturation of central nervous system than the birth weight. It seems that the neurological evaluation becomes unreliable in babies who suffer from intrauterine nutritional deprivation.", "contents": "Effect of intrauterine nutritional deprivation on neuromotor behaviour of the newborn. The neurological maturation in 25 newborn babies born to severely undernourished mothers was studied by evaluating muscle tone and excitability status. These mothers had weight below the 25th percentile expected for height, haemoglobin less than 80.0 g/l and serum albumin less than 25.0 g/l. Twenty-three babies born to healthy mothers were studied as control. The babies of undernourished mothers demonstrated gross intrauterine growth retardation. There occurred parallel reduction in placental weight and its protein content. The neuromotor behaviour of these newborns showed significant alteration in the performance of most reflexes, resembling normal motor behaviour of preterm infants. As many as 72% newborns could be classified as hypotonic and 56% hypoexcitable. However, no newborn demonstrated hypertonia or hyperexcitability. The parameters of neuromotor assessment were found to have no correlation with the birth weight in both undernourished as well as control group. These observations suggest that muscle tone and excitability are better indices of maturation of central nervous system than the birth weight. It seems that the neurological evaluation becomes unreliable in babies who suffer from intrauterine nutritional deprivation."}
{"id": "PMID:463539", "title": "Evidence of riboflavin depletion in breast-fed newborns and its further acceleration during treatment of hyperbilirubinemia by phototherapy.", "content": "Phototherapy in the treatment of newborns with hyperbilirubinemia, resulting in degradation of bilirubin, also appears to have other photodynamic effects on metabolism. We studied flavin adenine dinucleotide (FAD) saturation of erythrocyte glutathione reductase, which should reflect riboflavin nutritional status, in 28 healthy newborns, and followed 37 newborns with hyperbilirubinemia prior to the start of and during phototherapy. The results indicate that healthy newborns on human milk feeding, relatively poor in riboflavin, have evidence of a transient riboflavin depletion soon after birth. This effect is made more pronounced by phototherapy and partially prevented by parenteral or oral administration of moderate amounts of riboflavin.", "contents": "Evidence of riboflavin depletion in breast-fed newborns and its further acceleration during treatment of hyperbilirubinemia by phototherapy. Phototherapy in the treatment of newborns with hyperbilirubinemia, resulting in degradation of bilirubin, also appears to have other photodynamic effects on metabolism. We studied flavin adenine dinucleotide (FAD) saturation of erythrocyte glutathione reductase, which should reflect riboflavin nutritional status, in 28 healthy newborns, and followed 37 newborns with hyperbilirubinemia prior to the start of and during phototherapy. The results indicate that healthy newborns on human milk feeding, relatively poor in riboflavin, have evidence of a transient riboflavin depletion soon after birth. This effect is made more pronounced by phototherapy and partially prevented by parenteral or oral administration of moderate amounts of riboflavin."}
{"id": "PMID:463540", "title": "A longitudinal study of manganese in human milk.", "content": "Flameless atomic absorption spectrophotometry has been applied to the analysis of manganese in human milk samples. Twenty-seven breast-feeding mothers donated 229 individual milk samples between the 2nd week and the 9th month of lactation. The milk samples were representative of every feed in a period of 24 hours, foremilk and hind-milk in equal proportions. The median concentration of manganese declined from the initial value of 5.9 microgram/l to about 4 microgram/l before the 2nd month of lactation, remained at this level up to the 5th--6th month of lactation and showed a tendency to rise thereafter. The values presented here are noticeably lower than most of those reported earlier. It remains an open question whether the manganese concentration is exceptionally low in Finnish human milk or whether the great difference from most earlier studies may reflect problems of contamination or technical difficulties when less sensitive analytical methods have been used.", "contents": "A longitudinal study of manganese in human milk. Flameless atomic absorption spectrophotometry has been applied to the analysis of manganese in human milk samples. Twenty-seven breast-feeding mothers donated 229 individual milk samples between the 2nd week and the 9th month of lactation. The milk samples were representative of every feed in a period of 24 hours, foremilk and hind-milk in equal proportions. The median concentration of manganese declined from the initial value of 5.9 microgram/l to about 4 microgram/l before the 2nd month of lactation, remained at this level up to the 5th--6th month of lactation and showed a tendency to rise thereafter. The values presented here are noticeably lower than most of those reported earlier. It remains an open question whether the manganese concentration is exceptionally low in Finnish human milk or whether the great difference from most earlier studies may reflect problems of contamination or technical difficulties when less sensitive analytical methods have been used."}
{"id": "PMID:463541", "title": "Umbilical artery catheterization in newborns. I. Thrombosis in relation to catheter type and position.", "content": "Seventy-one sick newborn infants, who had an umbilical artery catheterized, were randomized in one of four catheter groups: long end-hole-, short end-hole-, long side-hole- or short side-hole catheter. A long catheter means a high position of the catheter tip (Th6--11) and a short catheter a low position of the tip (L3--5). An angiography through the indwelling catheter in order to diagnose thrombosis was performed before the catheter was withdrawn. Dissection of the aorta and its brances was performed on infants who died. The total frequency of thromboses was 26%. There were no thromboses among infants with long end-hole catheters while infants with short end-hole catheters had thrombosis in 26%, long side-hole catheters in 33% and short side-hole catheters in 64%. Long end-hole catheters functioned better than the others. Only 6 of 16 infants with thrombosis had physical signs from the legs, while 12 infants without thrombosis had similar signs.", "contents": "Umbilical artery catheterization in newborns. I. Thrombosis in relation to catheter type and position. Seventy-one sick newborn infants, who had an umbilical artery catheterized, were randomized in one of four catheter groups: long end-hole-, short end-hole-, long side-hole- or short side-hole catheter. A long catheter means a high position of the catheter tip (Th6--11) and a short catheter a low position of the tip (L3--5). An angiography through the indwelling catheter in order to diagnose thrombosis was performed before the catheter was withdrawn. Dissection of the aorta and its brances was performed on infants who died. The total frequency of thromboses was 26%. There were no thromboses among infants with long end-hole catheters while infants with short end-hole catheters had thrombosis in 26%, long side-hole catheters in 33% and short side-hole catheters in 64%. Long end-hole catheters functioned better than the others. Only 6 of 16 infants with thrombosis had physical signs from the legs, while 12 infants without thrombosis had similar signs."}
{"id": "PMID:463542", "title": "Adynamia episodica hereditaria. Treatment with salbutamol.", "content": "Three sibs with adynamia episodica hereditaria are described. Treatment with salbutamol inhalation had a beneficial effect on the duration of their adynamic attacks. Continuous peroral treatment with salbutamol has been tried in these patients resulting in the almost complete prevention of attacks. No serious adverse effects have been recorded. Peroral treatment with salbutamol is recommended as the treatment of choice in young patients with adynamia episodica hereditaria and in patients with frequent attacks of adynamia.", "contents": "Adynamia episodica hereditaria. Treatment with salbutamol. Three sibs with adynamia episodica hereditaria are described. Treatment with salbutamol inhalation had a beneficial effect on the duration of their adynamic attacks. Continuous peroral treatment with salbutamol has been tried in these patients resulting in the almost complete prevention of attacks. No serious adverse effects have been recorded. Peroral treatment with salbutamol is recommended as the treatment of choice in young patients with adynamia episodica hereditaria and in patients with frequent attacks of adynamia."}
{"id": "PMID:463543", "title": "Peak expiratory flow rate. Reference values for Swedish children.", "content": "Reference values for the peak expiratory flow rate assessed by the Wright-McKerrow peak flow meter have been established for Swedish children. The material consisted of 143 boys and 132 girls. We recommend the sexes be considered together. The equation of the regression line is 72.14 x height3 + 96.12. The coefficient of correlation is 0.93 and the residual standard deviation 13.7%.", "contents": "Peak expiratory flow rate. Reference values for Swedish children. Reference values for the peak expiratory flow rate assessed by the Wright-McKerrow peak flow meter have been established for Swedish children. The material consisted of 143 boys and 132 girls. We recommend the sexes be considered together. The equation of the regression line is 72.14 x height3 + 96.12. The coefficient of correlation is 0.93 and the residual standard deviation 13.7%."}
{"id": "PMID:463544", "title": "Hemoglobin A1c (HbA1c) in children with long standing and newly diagnosed diabetes mellitus.", "content": "In 35 children with long-standing diabetes mellitus, a significant correlation was found between the hemoglobin A1c (HbA1c)--and the 24-hour urinary glucose excretion. By contrast, 11 newly diagnosed diabetic children had grossly elevated HbA1c-concentrations, but no correlations could be established between the levels of HbA1c and the duration of symptoms, blood glucose, glycosuria, ketonuria and the acid--base status. However, HbA1c and C-peptide were significantly correlated. The elevated HbA1c-concentrations decreased towards normal in all of these 11 children after 2--3 months following adequate therapy. The results suggest that the determination of HbA1c may serve as a valuable metabolic control index in children with long-standing diabetes mellitus, but adds little information in newly diagnosed patients. For the individual diabetic child during the early treatment period, HbA1c may be the index of choice for adequacy of metabolic control.", "contents": "Hemoglobin A1c (HbA1c) in children with long standing and newly diagnosed diabetes mellitus. In 35 children with long-standing diabetes mellitus, a significant correlation was found between the hemoglobin A1c (HbA1c)--and the 24-hour urinary glucose excretion. By contrast, 11 newly diagnosed diabetic children had grossly elevated HbA1c-concentrations, but no correlations could be established between the levels of HbA1c and the duration of symptoms, blood glucose, glycosuria, ketonuria and the acid--base status. However, HbA1c and C-peptide were significantly correlated. The elevated HbA1c-concentrations decreased towards normal in all of these 11 children after 2--3 months following adequate therapy. The results suggest that the determination of HbA1c may serve as a valuable metabolic control index in children with long-standing diabetes mellitus, but adds little information in newly diagnosed patients. For the individual diabetic child during the early treatment period, HbA1c may be the index of choice for adequacy of metabolic control."}
{"id": "PMID:463549", "title": "Spontaneous perforation of the common bile duct.", "content": "An 18-week-old baby boy suffered an episode of acute abdominal symptoms followed by a silent period with mild obstructive jaundice, abdominal distension and failure to thrive. During the clinical work-up he deteriorated suddenly, with progressive abdominal distension and ascites. At laparotomy a perforation of the common bile duct with bile peritonitis was found. Spontaneous perforation of the common bile duct is seldom listed as a cause of obstructive jaundice. The clinical picture is characteristic. Awareness of this diagnosis may help to avoid time-consuming and unnecessary investigations and to lead to surgical treatment in good time.", "contents": "Spontaneous perforation of the common bile duct. An 18-week-old baby boy suffered an episode of acute abdominal symptoms followed by a silent period with mild obstructive jaundice, abdominal distension and failure to thrive. During the clinical work-up he deteriorated suddenly, with progressive abdominal distension and ascites. At laparotomy a perforation of the common bile duct with bile peritonitis was found. Spontaneous perforation of the common bile duct is seldom listed as a cause of obstructive jaundice. The clinical picture is characteristic. Awareness of this diagnosis may help to avoid time-consuming and unnecessary investigations and to lead to surgical treatment in good time."}
{"id": "PMID:463550", "title": "Scleroderma with massive regional lymphadenopathy.", "content": "Scleroderma in a two-year-old boy with gross enlargement of the right inguinal lymph nodes, as an early sign, is reported. Repeated lymph node biopsies revealed non-characteristic reactive changes and hyperplasia, but eventually histological examination of skin and muscle from the right leg were diagnostic. The effect of one year of D-penicillamine therapy is briefly mentioned.", "contents": "Scleroderma with massive regional lymphadenopathy. Scleroderma in a two-year-old boy with gross enlargement of the right inguinal lymph nodes, as an early sign, is reported. Repeated lymph node biopsies revealed non-characteristic reactive changes and hyperplasia, but eventually histological examination of skin and muscle from the right leg were diagnostic. The effect of one year of D-penicillamine therapy is briefly mentioned."}
{"id": "PMID:463551", "title": "Nonketotic hyperglycinemia. Clinical, biochemical and therapeutic aspects.", "content": "A patient exhibiting progressive cerebral depression from the first days of life is described. The diagnosis of nonketotic hyperglycinemia was established on the typical clinical presentation, elevated glycine concentrations in body fluids and diminished glycine cleavage activity in liver tissue. A series of therapeutic trials, including strychnine treatment, was tried on this patient without apparent effect on the clinical course.", "contents": "Nonketotic hyperglycinemia. Clinical, biochemical and therapeutic aspects. A patient exhibiting progressive cerebral depression from the first days of life is described. The diagnosis of nonketotic hyperglycinemia was established on the typical clinical presentation, elevated glycine concentrations in body fluids and diminished glycine cleavage activity in liver tissue. A series of therapeutic trials, including strychnine treatment, was tried on this patient without apparent effect on the clinical course."}
{"id": "PMID:463552", "title": "Deletion of the long arm of chromosome 11: a clinical entity.", "content": "A deletion of the long arm of chromosome No. 11, an aberration undetectable in conventional chromosome staining, was identified with Giemsa banding in a female infant with multiple congenital anomalies. A survey is given of the clinical findings in the few cases so far reported.", "contents": "Deletion of the long arm of chromosome 11: a clinical entity. A deletion of the long arm of chromosome No. 11, an aberration undetectable in conventional chromosome staining, was identified with Giemsa banding in a female infant with multiple congenital anomalies. A survey is given of the clinical findings in the few cases so far reported."}
{"id": "PMID:463553", "title": "Cellular aspects of rabbit Masugi nephritis. IV. Cell quantitation of proliferative glomerulonephritis.", "content": "Diffuse proliferative glomerulonephritis was induced in the rabbit with injection of goat nephrotoxic serum, and cell dynamics in the glomerulus were quantitatively investigated. The mean total cell number of a normal glomerulus in cross-section was 54.8, consisting of 24.1 endothelial cells, 16.0 podocytes, and 14.3 mesangial cells. In 5 selected nephritic rabbits, the mean total cell number of a glomerulus at the florid stage (7 to 12 experimental days) was 2.4 times as great as the control. It was revealed that the cells consisted of 27.9 endothelial cells, 14.9 podocytes, 21.1 mesangial cells, and 57.8 monocytic cells, indicating that about 76 per cent of the increased cells were of monocytic origin. Mesangial cells showed moderate increase in number (1.5 times) which persisted until 100 days, whereas endothelial cells increased only slightly. The monocytic cells promptly disappeared at 40 days correlating well with resolution of the proliferative change. Although the cellular compositions varied in each case, it was concluded that monocytes were essential in producing proliferative glomerular changes in the reversible type of Masugi nephritis.", "contents": "Cellular aspects of rabbit Masugi nephritis. IV. Cell quantitation of proliferative glomerulonephritis. Diffuse proliferative glomerulonephritis was induced in the rabbit with injection of goat nephrotoxic serum, and cell dynamics in the glomerulus were quantitatively investigated. The mean total cell number of a normal glomerulus in cross-section was 54.8, consisting of 24.1 endothelial cells, 16.0 podocytes, and 14.3 mesangial cells. In 5 selected nephritic rabbits, the mean total cell number of a glomerulus at the florid stage (7 to 12 experimental days) was 2.4 times as great as the control. It was revealed that the cells consisted of 27.9 endothelial cells, 14.9 podocytes, 21.1 mesangial cells, and 57.8 monocytic cells, indicating that about 76 per cent of the increased cells were of monocytic origin. Mesangial cells showed moderate increase in number (1.5 times) which persisted until 100 days, whereas endothelial cells increased only slightly. The monocytic cells promptly disappeared at 40 days correlating well with resolution of the proliferative change. Although the cellular compositions varied in each case, it was concluded that monocytes were essential in producing proliferative glomerular changes in the reversible type of Masugi nephritis."}
{"id": "PMID:463554", "title": "The development of extranodal lymphoid follicles in experimental bronchopneumonia.", "content": "Extranodal formation of lymphoid follicles was morphologically studied in experimental bronchopneumonia. Control gnotobiotic mice had no peribronchial lymphoid follicles and only lymphatic vessels were traced from the terminal bronchiolar region toward larger bronchi. During the week after intranasal inoculation of mycoplasma pulmonis, lymphoid follicles developed in the terminal portion of the lymphatics by the accumulation of small lymphocytes. A loose network of mesenchymal cells and early infiltration of macrophages, following stromal edema, seemed to play an important role in the early accumulation of lymphocytes. Blastic transformation was seen frequently in the center of the accumulated lymphocytes. Two weeks after inoculation plasma cells emerged conspicuously in the periphery of the lymphoid follicles, and the acute phase of bronchopneumonia began to subside. Typical germinal centers with tingible body macrophages and dendritic reticulum cells developed when the bronchitis persisted in a chronical manner.", "contents": "The development of extranodal lymphoid follicles in experimental bronchopneumonia. Extranodal formation of lymphoid follicles was morphologically studied in experimental bronchopneumonia. Control gnotobiotic mice had no peribronchial lymphoid follicles and only lymphatic vessels were traced from the terminal bronchiolar region toward larger bronchi. During the week after intranasal inoculation of mycoplasma pulmonis, lymphoid follicles developed in the terminal portion of the lymphatics by the accumulation of small lymphocytes. A loose network of mesenchymal cells and early infiltration of macrophages, following stromal edema, seemed to play an important role in the early accumulation of lymphocytes. Blastic transformation was seen frequently in the center of the accumulated lymphocytes. Two weeks after inoculation plasma cells emerged conspicuously in the periphery of the lymphoid follicles, and the acute phase of bronchopneumonia began to subside. Typical germinal centers with tingible body macrophages and dendritic reticulum cells developed when the bronchitis persisted in a chronical manner."}
{"id": "PMID:463556", "title": "Malignant neurofibroma with glandular differentiation (glandular schwannoma).", "content": "A case of malignant shwannoma is reported with unusual elements in an 89-year-old female. A large mass was located in the subcutaneous tissue of the right lateral chest wall and measured 5 cm in the greatest diameter. Histologically the tumor was composed of neurofibroma and malignant schwannoma with glandular differentiation. Neurofibroma characterized by numerous hyaline neural nodules was located in the peripheral portion of the tumor, whereas malignant schwannoma occupied a large part of the central portion of the tumor. The glandular elements observed in some areas of malignant schwannoma consisted of cuboidal and columnar shaped cells and were arranged in tubular or tubulo-medullary fashion in which rosettes or pseudorosettes were found. Mucicarminophilic material was observed, both in the cytoplasm and in the lumen. Seven reported cases of peripheral nerve tumor with glandular differentiation are reviewed briefly.", "contents": "Malignant neurofibroma with glandular differentiation (glandular schwannoma). A case of malignant shwannoma is reported with unusual elements in an 89-year-old female. A large mass was located in the subcutaneous tissue of the right lateral chest wall and measured 5 cm in the greatest diameter. Histologically the tumor was composed of neurofibroma and malignant schwannoma with glandular differentiation. Neurofibroma characterized by numerous hyaline neural nodules was located in the peripheral portion of the tumor, whereas malignant schwannoma occupied a large part of the central portion of the tumor. The glandular elements observed in some areas of malignant schwannoma consisted of cuboidal and columnar shaped cells and were arranged in tubular or tubulo-medullary fashion in which rosettes or pseudorosettes were found. Mucicarminophilic material was observed, both in the cytoplasm and in the lumen. Seven reported cases of peripheral nerve tumor with glandular differentiation are reviewed briefly."}
{"id": "PMID:463555", "title": "Immunopathologic studies of renal glomerular change in liver cirrhosis with special reference to its pathogenesis.", "content": "The kidneys from 62 proven liver cirrhosis cases were examined by immunofluorescence (IF), and 94% of the cases were positive for some immune reactants deposition. Combined deposition of immunoglobulin(s), both or each one of C1q and C4, and further with C3 were observed in about 70% of all IF positive cases. The morphological alterations of the glomeruli correlated with the intensities of regional immune reactants depositions. Guinea pig C3 was frequently activated in vitro on the glomeruli of these cases. Immune reactants depositions in the glomeruli appeared to form immune complex locally. Smooth muscle and liver cell antigens in the immune complex at the glomeruli were examined by indirect method of IF using monospecific antibodies and positive cases concerning each antigen were found in about 1/3 of the kidneys from 21 liver cirrhosis cases. These facts suggest that the high rate of the occurrence of immune complex deposition type glomerulo-nephritis may be due to the glomerular deposition of some autoantigen-antibody complexes including smooth muscle and liver cell antigens.", "contents": "Immunopathologic studies of renal glomerular change in liver cirrhosis with special reference to its pathogenesis. The kidneys from 62 proven liver cirrhosis cases were examined by immunofluorescence (IF), and 94% of the cases were positive for some immune reactants deposition. Combined deposition of immunoglobulin(s), both or each one of C1q and C4, and further with C3 were observed in about 70% of all IF positive cases. The morphological alterations of the glomeruli correlated with the intensities of regional immune reactants depositions. Guinea pig C3 was frequently activated in vitro on the glomeruli of these cases. Immune reactants depositions in the glomeruli appeared to form immune complex locally. Smooth muscle and liver cell antigens in the immune complex at the glomeruli were examined by indirect method of IF using monospecific antibodies and positive cases concerning each antigen were found in about 1/3 of the kidneys from 21 liver cirrhosis cases. These facts suggest that the high rate of the occurrence of immune complex deposition type glomerulo-nephritis may be due to the glomerular deposition of some autoantigen-antibody complexes including smooth muscle and liver cell antigens."}
{"id": "PMID:463557", "title": "Different histological manifestation of glomerular lesions in familial systemic lupus erythematosus.", "content": "Lupus nephritis in a sister and brother pair was histologically analyzed. The 12-year-old sister showed clinically progressive renal failure and her renal pathology disclosed segmental necrotizing and disorganizing glomerulonephritis. On the contrary, the 14-year-old brother exhibited nephrotic syndrome and his renal biopsy showed diffuse exudative and proliferative glomerulonephritis with segmental membraneous changes. Although the familial incidence of SLE suggests both genetic and environmental factors existing in the background of SLE of identical twins and siblings, the differences of clinical manifestation and histopathological findings imply that there might be little direct genetic and environmental control on determining the expression of the disease.", "contents": "Different histological manifestation of glomerular lesions in familial systemic lupus erythematosus. Lupus nephritis in a sister and brother pair was histologically analyzed. The 12-year-old sister showed clinically progressive renal failure and her renal pathology disclosed segmental necrotizing and disorganizing glomerulonephritis. On the contrary, the 14-year-old brother exhibited nephrotic syndrome and his renal biopsy showed diffuse exudative and proliferative glomerulonephritis with segmental membraneous changes. Although the familial incidence of SLE suggests both genetic and environmental factors existing in the background of SLE of identical twins and siblings, the differences of clinical manifestation and histopathological findings imply that there might be little direct genetic and environmental control on determining the expression of the disease."}
{"id": "PMID:463558", "title": "Tyrosinemia.", "content": "A case of tyrosinemia in a three-month-old boy is presented. The patient appeared jaundiced initially with markedly elevated levels of serum tyrosine and a positive Millon-reacting urine. Jaundice persisted and hepatosplenomegaly gradually increased. He died due to liver failure on the 51st day after admission. At autopsy, the liver showed the features of severe giant cell hepatitis including giant cell formation, fibrosis and bile retention. The pancreas and the brain showed characteristic postmortem findings as previously reported  in patients with tyrosinemia.", "contents": "Tyrosinemia. A case of tyrosinemia in a three-month-old boy is presented. The patient appeared jaundiced initially with markedly elevated levels of serum tyrosine and a positive Millon-reacting urine. Jaundice persisted and hepatosplenomegaly gradually increased. He died due to liver failure on the 51st day after admission. At autopsy, the liver showed the features of severe giant cell hepatitis including giant cell formation, fibrosis and bile retention. The pancreas and the brain showed characteristic postmortem findings as previously reported  in patients with tyrosinemia."}
{"id": "PMID:463559", "title": "Nonpapillary carcinoma in situ of the urinary bladder. A histopathologic study and mapping of the urothelial lesions.", "content": "Three cases of primary nonpapillary carcinoma of the urinary bladder diagnosed by urinary cytology and multiple biopsies were reported. Bladder specimens of two of the patients were totally embedded for step-sections that were mapped after histopathologic study. Atypical hyperplasia and carcinoma in situ with foci of microscopic invasion affected the bladder mucosa and extended continuously to the distal ureters as well as prostatic urethra. Multicentric distribuion of the abnormal epithelium was definite in one case, and the bladder mucosa was extensively denuded in the other case. Metastasis to one of the regional lymph nodes was noted in the remaining one case. The origin of Pagetoid cells occurring in two of the cases is obscure, but we presume that these cells may represent transformed tumor cells showing differentiation toward the surface umbrella cells, or they are derived from Brunn's nests where the cells may gain potential to differentiate to glandular epithelium.", "contents": "Nonpapillary carcinoma in situ of the urinary bladder. A histopathologic study and mapping of the urothelial lesions. Three cases of primary nonpapillary carcinoma of the urinary bladder diagnosed by urinary cytology and multiple biopsies were reported. Bladder specimens of two of the patients were totally embedded for step-sections that were mapped after histopathologic study. Atypical hyperplasia and carcinoma in situ with foci of microscopic invasion affected the bladder mucosa and extended continuously to the distal ureters as well as prostatic urethra. Multicentric distribuion of the abnormal epithelium was definite in one case, and the bladder mucosa was extensively denuded in the other case. Metastasis to one of the regional lymph nodes was noted in the remaining one case. The origin of Pagetoid cells occurring in two of the cases is obscure, but we presume that these cells may represent transformed tumor cells showing differentiation toward the surface umbrella cells, or they are derived from Brunn's nests where the cells may gain potential to differentiate to glandular epithelium."}
{"id": "PMID:463560", "title": "Non-traumatic uriniferous pseudocyst. Report of a case.", "content": "A non-traumatic uriniferous pseudocyst was incidentally found at autopsy in a patient with invasive urothelial carcinoma involving ureteral orifices. Massive paraaortic lymph node involvement by the metastic tumor in addition to the ureteral obstruction was considered responsible for the formation of uriniferous pseudocyst. The pertinent clinical history and autopsy findings are presented and a possible mechanism of pseudocyst formation is discussed.", "contents": "Non-traumatic uriniferous pseudocyst. Report of a case. A non-traumatic uriniferous pseudocyst was incidentally found at autopsy in a patient with invasive urothelial carcinoma involving ureteral orifices. Massive paraaortic lymph node involvement by the metastic tumor in addition to the ureteral obstruction was considered responsible for the formation of uriniferous pseudocyst. The pertinent clinical history and autopsy findings are presented and a possible mechanism of pseudocyst formation is discussed."}
{"id": "PMID:463561", "title": "C cell carcinoma of the thyroid--papillary type.", "content": "Two cases of papillary type of C cell carcinoma of the thyroid were reported. They showed papillary arrangement with fibrovascular stalk in properly fixed tissues. Histochemically argyrophil reaction was positive in the cytoplasm and amyloid deposited in the stroma. Ultrastructurally secretory granules were found in their cytoplasm. The papillary type is not an artifact but one of the histologic variations of this carcinoma.", "contents": "C cell carcinoma of the thyroid--papillary type. Two cases of papillary type of C cell carcinoma of the thyroid were reported. They showed papillary arrangement with fibrovascular stalk in properly fixed tissues. Histochemically argyrophil reaction was positive in the cytoplasm and amyloid deposited in the stroma. Ultrastructurally secretory granules were found in their cytoplasm. The papillary type is not an artifact but one of the histologic variations of this carcinoma."}
{"id": "PMID:463562", "title": "Prolactin and 3-methylcholanthrene induced cervical carcinoma. Effect of bromocriptine.", "content": "Female mice of the NMRI strain were injected with estradil for the first five days after birth (estrogenized animals) and ovariectomized at the ages of 6-9 weeks. One week later, a cotton thread impregnated with 3-methylcholanthrene was inserted into the uterine cervix. Starting on the day of insertion of the thread, and for a further 6 days, the females were injected with estradiol (E2), ovine prolactin (P), or 2-bromo-alpha-ergokryptine mesylate (bromocriptine). Controls were injected with vehicles only. The animals were killed 4 or 8 weeks after insertion of the thread, and the uterine cervix was serially sectioned. A combined treatment with E2 and P resulted in an increased incidence of invasive epithelial lesions in the uterine cervix. This incidence was higher than in controls or females injected with either hormone separately. Bromocriptine reduced the incidence of invasions, and this reduction with either hormone separately. Bromocriptine reduced the incidence of invasion, and this reduction could not be restored to the contrl level by a simultaneous treatment with E2 anrogenized females was higher than that reported in an earlied study using non-estrogpenized females.", "contents": "Prolactin and 3-methylcholanthrene induced cervical carcinoma. Effect of bromocriptine. Female mice of the NMRI strain were injected with estradil for the first five days after birth (estrogenized animals) and ovariectomized at the ages of 6-9 weeks. One week later, a cotton thread impregnated with 3-methylcholanthrene was inserted into the uterine cervix. Starting on the day of insertion of the thread, and for a further 6 days, the females were injected with estradiol (E2), ovine prolactin (P), or 2-bromo-alpha-ergokryptine mesylate (bromocriptine). Controls were injected with vehicles only. The animals were killed 4 or 8 weeks after insertion of the thread, and the uterine cervix was serially sectioned. A combined treatment with E2 and P resulted in an increased incidence of invasive epithelial lesions in the uterine cervix. This incidence was higher than in controls or females injected with either hormone separately. Bromocriptine reduced the incidence of invasions, and this reduction with either hormone separately. Bromocriptine reduced the incidence of invasion, and this reduction could not be restored to the contrl level by a simultaneous treatment with E2 anrogenized females was higher than that reported in an earlied study using non-estrogpenized females."}
{"id": "PMID:463563", "title": "Response of pulmonary (circulating) megakaryocytes to experimentally induced consumption coagulopathy in rabbits.", "content": "The effects of slow temporary infusion of a tissue thromboplastin solution into the superior vena cava on pulmonary as well as circulating megakaryocytes were studied in 40 rabbits (2-48 hours after infusion) and related to 6 noninfused and 7 infused with normal saline. This is a simple and specific method of inducing a fall in blood platelet levels and thereby an activation of thrombocytopoiesis and megakaryocytopoiesis. The induced intravascular coagulation is probably counterbalanced by an activated fibrinolysis allowing the animals to survive the infusion and thereby offering the possibility of studying the long-term effects. An increase to about  300% of the normal values in circulating as well as pulmonary megakaryocytes was found 20 and 24 hours after the onset of the infusions respectively. The number of circulating and pulmonary megakaryocytes, showing great individual variations, however, dropped to normal levels within 28 hours after onset of the infusions, which means that megakaryocytes remain detectable for less than eight hours in the lungs. No increase was found in pulmonary megakaryocytes in the control (saline infused) group. In our opinion the entrance of megakaryocytes from the bone marrow into the blood circulation in an incidental event, the number in the circulation reflecting the activity of megakarycocytopoiesis. This experiment supports our suggestion that intravascular coagulation is one of the major pathophysiological mechanisms leading to an increase in pulmonary megakaryocytes.", "contents": "Response of pulmonary (circulating) megakaryocytes to experimentally induced consumption coagulopathy in rabbits. The effects of slow temporary infusion of a tissue thromboplastin solution into the superior vena cava on pulmonary as well as circulating megakaryocytes were studied in 40 rabbits (2-48 hours after infusion) and related to 6 noninfused and 7 infused with normal saline. This is a simple and specific method of inducing a fall in blood platelet levels and thereby an activation of thrombocytopoiesis and megakaryocytopoiesis. The induced intravascular coagulation is probably counterbalanced by an activated fibrinolysis allowing the animals to survive the infusion and thereby offering the possibility of studying the long-term effects. An increase to about  300% of the normal values in circulating as well as pulmonary megakaryocytes was found 20 and 24 hours after the onset of the infusions respectively. The number of circulating and pulmonary megakaryocytes, showing great individual variations, however, dropped to normal levels within 28 hours after onset of the infusions, which means that megakaryocytes remain detectable for less than eight hours in the lungs. No increase was found in pulmonary megakaryocytes in the control (saline infused) group. In our opinion the entrance of megakaryocytes from the bone marrow into the blood circulation in an incidental event, the number in the circulation reflecting the activity of megakarycocytopoiesis. This experiment supports our suggestion that intravascular coagulation is one of the major pathophysiological mechanisms leading to an increase in pulmonary megakaryocytes."}
{"id": "PMID:463564", "title": "Effects of cyclophosphamide on open, granulating skin wounds in rats.", "content": "The effect of cyclophosphamide on the healing of open cutaneous wounds was studied in rats. Following intraperitoneal injections of 25 mg/kg body weight every second day for 9 days, only about 7% of the wounds were completely covered by epithelium after 15 days, whereas in the control animals 60% of the wounds were completely epithelialized. Measurements of wound diameters in circular skin wounds revealed unhealed wound areas in the drug treated animals which were significantly larger than those of the control animals. Cyclophosphamide was found to reduce the occurrence of H3-labelled cells in the granulation tissue when evaluated after 11 days. At 15 days there was no difference in the labelling frequency between treated and control animals, indicating reversal of the drug effect.", "contents": "Effects of cyclophosphamide on open, granulating skin wounds in rats. The effect of cyclophosphamide on the healing of open cutaneous wounds was studied in rats. Following intraperitoneal injections of 25 mg/kg body weight every second day for 9 days, only about 7% of the wounds were completely covered by epithelium after 15 days, whereas in the control animals 60% of the wounds were completely epithelialized. Measurements of wound diameters in circular skin wounds revealed unhealed wound areas in the drug treated animals which were significantly larger than those of the control animals. Cyclophosphamide was found to reduce the occurrence of H3-labelled cells in the granulation tissue when evaluated after 11 days. At 15 days there was no difference in the labelling frequency between treated and control animals, indicating reversal of the drug effect."}
{"id": "PMID:463565", "title": "Mechanical properties and hydroxyproline content of connective tissue in porous ceramic implants.", "content": "The present study describes a model applying ceramic implants (A1203) for in vivo studies of connective tissue regeneration. Two types of implants have been developed: a one-piece model for histological examination and chemical analyses, and a two-piece implant which can also be used for mechanical testing of connective tissue. When these were implanted subcutaneoulsly on the back of rats, a correlation was found between the mechanical strength and the hydroxyproline content of connective tissue in the implants. The peak synthesis occurred between the 7th and the 14th day after implantation, and a plateau was reached for both strength-increases and hydroxyproline-formation between the 14th and the 21st days. For histological examination, the implants were embedded in plastic materials and prepared as hard tissue specimens. The model presented can be applied to study connective tissue regeneration in normal and pathological conditions, including studies of the effects of various drugs on the connective tissue.", "contents": "Mechanical properties and hydroxyproline content of connective tissue in porous ceramic implants. The present study describes a model applying ceramic implants (A1203) for in vivo studies of connective tissue regeneration. Two types of implants have been developed: a one-piece model for histological examination and chemical analyses, and a two-piece implant which can also be used for mechanical testing of connective tissue. When these were implanted subcutaneoulsly on the back of rats, a correlation was found between the mechanical strength and the hydroxyproline content of connective tissue in the implants. The peak synthesis occurred between the 7th and the 14th day after implantation, and a plateau was reached for both strength-increases and hydroxyproline-formation between the 14th and the 21st days. For histological examination, the implants were embedded in plastic materials and prepared as hard tissue specimens. The model presented can be applied to study connective tissue regeneration in normal and pathological conditions, including studies of the effects of various drugs on the connective tissue."}
{"id": "PMID:463566", "title": "Surface ultrastructure of human arteries with special reference to the effects of smoking.", "content": "The surface ultrastructure of uterine arteries from 20 women was studied with scanning electron microscopy. Specimens were excised and fixed under pressure shortely after removal of the uterus during hysterectomy. Two groups of patients were selected: non-smokers and women who smoked more than 15 cigarettes a day. The prevalence of seven different morphological criteria was evaluated without knowledge of the smoking habits of the patients. Endothelial cells were usually well demarcated and distributed with their length axis paralledl to that of the artery. The cell nucleus was revealed by a gentle bulging into the lumen, and cell borders by the presence of rows of microvilli. Inter- and intracellular holes alon the cell borders were more common in arteries from smokers, whereas microvillie were more common on the cell surfaces of arteries from non-smokers. These differences might be related to cell injury. Previous studies indicate that holes are more frequent in injured than in non-injured cells, whereas the surface of injured cells is often smoother than that of non-injured cells. Therefore, these data may be taken to suggest that smoking can lead to endothelial injury in man.", "contents": "Surface ultrastructure of human arteries with special reference to the effects of smoking. The surface ultrastructure of uterine arteries from 20 women was studied with scanning electron microscopy. Specimens were excised and fixed under pressure shortely after removal of the uterus during hysterectomy. Two groups of patients were selected: non-smokers and women who smoked more than 15 cigarettes a day. The prevalence of seven different morphological criteria was evaluated without knowledge of the smoking habits of the patients. Endothelial cells were usually well demarcated and distributed with their length axis paralledl to that of the artery. The cell nucleus was revealed by a gentle bulging into the lumen, and cell borders by the presence of rows of microvilli. Inter- and intracellular holes alon the cell borders were more common in arteries from smokers, whereas microvillie were more common on the cell surfaces of arteries from non-smokers. These differences might be related to cell injury. Previous studies indicate that holes are more frequent in injured than in non-injured cells, whereas the surface of injured cells is often smoother than that of non-injured cells. Therefore, these data may be taken to suggest that smoking can lead to endothelial injury in man."}
{"id": "PMID:463567", "title": "Structural changes and ability to release renin in auto- and allo-transplants of mouse submaxillary glands.", "content": "In mice having a high renin content in the submaxillary glands allo- and autotransplantation of the gland showed identical histological changes of the tissue, comprising disappearance of acini and intercalated ducts as well as a reduction in the number and size of granules in the granulated ducts. No structural signs of rejection were found. Adenomas, possibly originating in the granulated ducts, were frequently present in the transplanted glands. The renin content of autotransplanted glands was invariably much higher than in allotransplants, and after noradrenaline injection renin was released only from autotransplants, never from allotransplants. Blockade of the renin system was accordingly followed by a decrease in blood pressure only in mice with autotransplants.", "contents": "Structural changes and ability to release renin in auto- and allo-transplants of mouse submaxillary glands. In mice having a high renin content in the submaxillary glands allo- and autotransplantation of the gland showed identical histological changes of the tissue, comprising disappearance of acini and intercalated ducts as well as a reduction in the number and size of granules in the granulated ducts. No structural signs of rejection were found. Adenomas, possibly originating in the granulated ducts, were frequently present in the transplanted glands. The renin content of autotransplanted glands was invariably much higher than in allotransplants, and after noradrenaline injection renin was released only from autotransplants, never from allotransplants. Blockade of the renin system was accordingly followed by a decrease in blood pressure only in mice with autotransplants."}
{"id": "PMID:463568", "title": "Cell proliferation in normal and diseased gastric mucosa. Autoradiography after in vitro continuous labelling with tritiated thymidine.", "content": "The rate of gastric epithelial cell proliferation was studied in healthy volunteers and in patients with different degrees of gastritis. Endoscopic biopsies from the antral and fundic part of the stomach were incubated in vitro with 3H-thymidine for 30, 120, and 210 minutes respectively. Autoradiographs were prepared, and the percentage of DNA-synthesizing cells (labeling index) in the progenitor cell region was estimated. From the successive labeling indices the rate of entry of cells into DNA-synthetic phase (S-phase) and the duration of the S-phase could be estimated. All the biopsies were classified according to the degree of gastritis. The mean (+/-SEM) length of the S-phase was found to be 7.4 +/- 0.3 hours in antral mucosa and 7.2 +/- 0.4 hours in fundic mucosa. There was no significant difference between the S-phase duration in normal mucosa, superficial gastritis, mild atrophic gastritis and severe atrophic gastritis. This observation suggests that the labeling index can be used as an expression for the rate of cell proliferation in human gastric mucosa. A significant correlation between the labelling indices and the degree of gastritis was found in both antral and fundic mucosa. In six cases, labelling indices estimated by cell counts performed on longitudinal or cross sections of foveolae were compared. Ther was no significant difference between the results obtained by the two different counting techniques.", "contents": "Cell proliferation in normal and diseased gastric mucosa. Autoradiography after in vitro continuous labelling with tritiated thymidine. The rate of gastric epithelial cell proliferation was studied in healthy volunteers and in patients with different degrees of gastritis. Endoscopic biopsies from the antral and fundic part of the stomach were incubated in vitro with 3H-thymidine for 30, 120, and 210 minutes respectively. Autoradiographs were prepared, and the percentage of DNA-synthesizing cells (labeling index) in the progenitor cell region was estimated. From the successive labeling indices the rate of entry of cells into DNA-synthetic phase (S-phase) and the duration of the S-phase could be estimated. All the biopsies were classified according to the degree of gastritis. The mean (+/-SEM) length of the S-phase was found to be 7.4 +/- 0.3 hours in antral mucosa and 7.2 +/- 0.4 hours in fundic mucosa. There was no significant difference between the S-phase duration in normal mucosa, superficial gastritis, mild atrophic gastritis and severe atrophic gastritis. This observation suggests that the labeling index can be used as an expression for the rate of cell proliferation in human gastric mucosa. A significant correlation between the labelling indices and the degree of gastritis was found in both antral and fundic mucosa. In six cases, labelling indices estimated by cell counts performed on longitudinal or cross sections of foveolae were compared. Ther was no significant difference between the results obtained by the two different counting techniques."}
{"id": "PMID:463573", "title": "Diaphragmatic blood flow at various levels of ventilation in the rabbit.", "content": "Diaphragmatic and renal blood flow were measured with Ytterbium-169 and Scandium 46 labelled 15 micron microspheres in sodium pentobarbitone anesthetized rabbits. The first measurement was performed during spontaneous breathing of air and the second measurement after 15 min of breathing 2-6% carbon dioxide in oxygen. The lung ventilation as well as the diaphragmatic blood flow increased significantly during breathing of the carbon dioxide-oxygen mixture. Arterial blood pressure and renal blood flow were not significantly altered by the induced hyperventilation. No significant correlation was found between the magnitude of lung ventilation and diaphragmatic blood flow. The results of the present study indicate that consecutive measurements of diaphragmatic blood flow with radioactive microspheres at various levels of breathing effort is an appropriate method for further exploration of the relationship between diaphragmatic perfusion and working performance.", "contents": "Diaphragmatic blood flow at various levels of ventilation in the rabbit. Diaphragmatic and renal blood flow were measured with Ytterbium-169 and Scandium 46 labelled 15 micron microspheres in sodium pentobarbitone anesthetized rabbits. The first measurement was performed during spontaneous breathing of air and the second measurement after 15 min of breathing 2-6% carbon dioxide in oxygen. The lung ventilation as well as the diaphragmatic blood flow increased significantly during breathing of the carbon dioxide-oxygen mixture. Arterial blood pressure and renal blood flow were not significantly altered by the induced hyperventilation. No significant correlation was found between the magnitude of lung ventilation and diaphragmatic blood flow. The results of the present study indicate that consecutive measurements of diaphragmatic blood flow with radioactive microspheres at various levels of breathing effort is an appropriate method for further exploration of the relationship between diaphragmatic perfusion and working performance."}
{"id": "PMID:463574", "title": "Evidence for a cholinergic secretory innervation of the guinea-pig endometrium.", "content": "Uterine mucus secretion was studied by measuring carbohydrate release from everted guinea-pig uteri placed in organ baths. Field stimulation of the organ resulted in a significant increase in carbohydrate out-put, this increase was inhibited by tetrodotoxin, scopolamine and previous destruction of the paracervical ganglia. Previous section of the hypogastric nerve reduced the response to field stimulation, while interruption of the costouterine connexion or the pelvic nerve was without overt effect. Stimulation of the hypogastric nerve increased uterine carbohydrate secretion. Pelvic nerve stimulation produced no significant effect. Carbachol increased the carbohydrate secretion to the same extent as did field stimulation. Isoprenaline and methoxamine were without significant effect. It is suggested that certain endometrial cells have a cholinergic sympathetic secretory innervation with pre- and postganglionic fibers running in the hypogastric nerve.", "contents": "Evidence for a cholinergic secretory innervation of the guinea-pig endometrium. Uterine mucus secretion was studied by measuring carbohydrate release from everted guinea-pig uteri placed in organ baths. Field stimulation of the organ resulted in a significant increase in carbohydrate out-put, this increase was inhibited by tetrodotoxin, scopolamine and previous destruction of the paracervical ganglia. Previous section of the hypogastric nerve reduced the response to field stimulation, while interruption of the costouterine connexion or the pelvic nerve was without overt effect. Stimulation of the hypogastric nerve increased uterine carbohydrate secretion. Pelvic nerve stimulation produced no significant effect. Carbachol increased the carbohydrate secretion to the same extent as did field stimulation. Isoprenaline and methoxamine were without significant effect. It is suggested that certain endometrial cells have a cholinergic sympathetic secretory innervation with pre- and postganglionic fibers running in the hypogastric nerve."}
{"id": "PMID:463575", "title": "Inhibition of renal sympathetic nerve traffic from cardiac receptors in normotensive and spontaneously hypertensive rats.", "content": "The reflex inhibition of the sympathetic outflow to the kidney was examined during volume load with horse plasma in 6 normotensive rats (NCR) and 6 spontaneously hypertensive rats (SRH). The rats were anesthetized with chloralose and urethane. The arterial baroreceptors were denervated. The renal nervous inhibition was mediated via the vagal nerves and was mainly due to activation of receptors in the left side of the heart. The average thresholds in mean left artrial pressure for renal nervous inhibition was 5.4 mmHg for NCR and 9.2 mmHg for SHR indicating a clear resetting of the reflex arch in the hypertensive animal: The reason is probably a decreased distensibility of the wall of the left atrium due to a chronic elevation of left atrial pressure. This resetting of the atrial receptors in the hypertensive animals is probably of importance to allow an adequate filling pressure of the hypertrophied left ventricle and might also be of importance for the reflex neural control of renal function in these animals.", "contents": "Inhibition of renal sympathetic nerve traffic from cardiac receptors in normotensive and spontaneously hypertensive rats. The reflex inhibition of the sympathetic outflow to the kidney was examined during volume load with horse plasma in 6 normotensive rats (NCR) and 6 spontaneously hypertensive rats (SRH). The rats were anesthetized with chloralose and urethane. The arterial baroreceptors were denervated. The renal nervous inhibition was mediated via the vagal nerves and was mainly due to activation of receptors in the left side of the heart. The average thresholds in mean left artrial pressure for renal nervous inhibition was 5.4 mmHg for NCR and 9.2 mmHg for SHR indicating a clear resetting of the reflex arch in the hypertensive animal: The reason is probably a decreased distensibility of the wall of the left atrium due to a chronic elevation of left atrial pressure. This resetting of the atrial receptors in the hypertensive animals is probably of importance to allow an adequate filling pressure of the hypertrophied left ventricle and might also be of importance for the reflex neural control of renal function in these animals."}
{"id": "PMID:463571", "title": "Reversible brain hyperthermia during audiogenic seizures in rats.", "content": "Reversible brain hyperthermia during audiogenic seizures in rats. Acta Physiol. Pol., 1979, 30 (2): 273--277. Nine rats having inborn audiogenic susceptibility had chronically implanted Cu-constantan thermocouple and epidural EEG electrodes sewed into the skull. In freely moving animals audiogenic seizures were provoked by the use of common electric bell (approx. 100 dB). The EEG trace and brain temperature (with the accuracy of 0.05 degrees C) were recorded simultaneously during 1--8 hours. Temperature increases found during 23 audiogenic seizures were similar to those noted during intensive locomotor activity of animals without seizures. In thermoneutral environment (23 +/- 2 degrees C and humidity 65% +/- 10%) brain temperature never exceeded 41 degrees C.", "contents": "Reversible brain hyperthermia during audiogenic seizures in rats. Reversible brain hyperthermia during audiogenic seizures in rats. Acta Physiol. Pol., 1979, 30 (2): 273--277. Nine rats having inborn audiogenic susceptibility had chronically implanted Cu-constantan thermocouple and epidural EEG electrodes sewed into the skull. In freely moving animals audiogenic seizures were provoked by the use of common electric bell (approx. 100 dB). The EEG trace and brain temperature (with the accuracy of 0.05 degrees C) were recorded simultaneously during 1--8 hours. Temperature increases found during 23 audiogenic seizures were similar to those noted during intensive locomotor activity of animals without seizures. In thermoneutral environment (23 +/- 2 degrees C and humidity 65% +/- 10%) brain temperature never exceeded 41 degrees C."}
{"id": "PMID:463576", "title": "The effect of noise on cochlear blood flow in the conscious rabbit.", "content": "The cochlear blood flow was measured with the microsphere method in unanesthetized rabbits, prior to and during noise-exposure. Different sound pressure levels were used: 100 dB, 115 dB and 120 dB. Some of the animals had the cervical sympathetic trunk cut unilaterally. The effects of the delivered noise on the organ of Corti were studied in other rabbits with scanning electron microscopy. In spite of ultrastructural changes indicating damage of sensory cells after 115 dB and 120 dB exposure no changes of the total cochlear blood flow could be observed. After unilateral section of the cervical sympathetic trunk the cochlear blood flow was less on the intact side (25%) already prior to noise exposure and the difference was not significantly affected by noise. Different sizes of microspheres were used: 8.2, 8.4, 8.7, 9,2 mum. The 8.2 and 8.4 mum-spheres seemed well fitted for measurements of cochlear blood flow but passed the cerebral capillaries to a high extent. Control experiments using both 8.2/8.4 mum-spheres and 15 mum-spheres were performed and the degree of passage in different tissues was estimated.", "contents": "The effect of noise on cochlear blood flow in the conscious rabbit. The cochlear blood flow was measured with the microsphere method in unanesthetized rabbits, prior to and during noise-exposure. Different sound pressure levels were used: 100 dB, 115 dB and 120 dB. Some of the animals had the cervical sympathetic trunk cut unilaterally. The effects of the delivered noise on the organ of Corti were studied in other rabbits with scanning electron microscopy. In spite of ultrastructural changes indicating damage of sensory cells after 115 dB and 120 dB exposure no changes of the total cochlear blood flow could be observed. After unilateral section of the cervical sympathetic trunk the cochlear blood flow was less on the intact side (25%) already prior to noise exposure and the difference was not significantly affected by noise. Different sizes of microspheres were used: 8.2, 8.4, 8.7, 9,2 mum. The 8.2 and 8.4 mum-spheres seemed well fitted for measurements of cochlear blood flow but passed the cerebral capillaries to a high extent. Control experiments using both 8.2/8.4 mum-spheres and 15 mum-spheres were performed and the degree of passage in different tissues was estimated."}
{"id": "PMID:463577", "title": "Metabolic responses in feline \"red\" and \"white\" skeletal muscle to shock and ischemia.", "content": "In order to investigate possible differences in the reaction to hypoxic conditions between \"red\" and \"white\" skeletal muscle, cats were subjected to a 2 h period of either hemorrhagic shock or hind limb tourniquet ischemia, and the hypoxia induced changes were studied in the soleus and lateral gastrocnemius muscles. Muscle biopsies were analysed for ATP, CP, glucose, G 6-P and lactate. Using microelectrodes, the resting membrane potential was repeatedly measured. Both experimental models resulted in increased tissue lactate levels and a successive decrease in the membrane potential of both muscles studied. No reduction of the high energy phosphagen content (ATP + CP) occurred in any of the muscles during shock. The tourniquet ischemia resulted in a 40% reduction of the ATP + CP content in the soleus muscle, whereas in the gastrocnemius muscle no significant reduction occurred. A significant correlation was found between the tissue lactate content and the membrane potential under both conditions and in both muscles studied. It is concluded that \"red\" muscles are more susceptible to metabolic derangement than \"white\" muscles during total ischemia, whereas during hypovolemia \"red\" muscles appear to be protected from early hypoxic damage, probably due to a redistribution of skeletal muscle blood flow.", "contents": "Metabolic responses in feline \"red\" and \"white\" skeletal muscle to shock and ischemia. In order to investigate possible differences in the reaction to hypoxic conditions between \"red\" and \"white\" skeletal muscle, cats were subjected to a 2 h period of either hemorrhagic shock or hind limb tourniquet ischemia, and the hypoxia induced changes were studied in the soleus and lateral gastrocnemius muscles. Muscle biopsies were analysed for ATP, CP, glucose, G 6-P and lactate. Using microelectrodes, the resting membrane potential was repeatedly measured. Both experimental models resulted in increased tissue lactate levels and a successive decrease in the membrane potential of both muscles studied. No reduction of the high energy phosphagen content (ATP + CP) occurred in any of the muscles during shock. The tourniquet ischemia resulted in a 40% reduction of the ATP + CP content in the soleus muscle, whereas in the gastrocnemius muscle no significant reduction occurred. A significant correlation was found between the tissue lactate content and the membrane potential under both conditions and in both muscles studied. It is concluded that \"red\" muscles are more susceptible to metabolic derangement than \"white\" muscles during total ischemia, whereas during hypovolemia \"red\" muscles appear to be protected from early hypoxic damage, probably due to a redistribution of skeletal muscle blood flow."}
{"id": "PMID:463572", "title": "Secretion of nitrogenous compounds in the small intestine of pigs.", "content": "Secretion of nitrogenous compounds in the small intestine of pigs. Acta Physiol. Pol., 1979, 30 (2): 319--326. The study was performed on pigs weighting 50 to 75 kg with temporarily isolated loops of one of the three parts of the small intestine: proximal, middle and terminal. For measurement of intestinal secretion the isolated loops were perfused with N-free solutions and the effluent was collected and analyzed. On a protein diet, under conditions of an isotonic osmotic pressure of the solution, secretion of nitrogen compounds in the upper part was twice that in the lower part of the small intestine, 0.97 against 0.48 g N/m/24 h. A hypertonic pressure increased secretion of N into the intestinal lumen. On a protein-free diet secretion of nitrogen compounds was markedly decreased. Soluble N amounted to about 90% of total N and alpha-amino-N to 50 to 79% of soluble N. Free amino acids made up about 65% and protein 33% of the total alpha-amino-N. There was a large proportion of threonine, 6.0--6.3%, and lysine, 8.0--8.7% in the total amino acids secreted into the intestine.", "contents": "Secretion of nitrogenous compounds in the small intestine of pigs. Secretion of nitrogenous compounds in the small intestine of pigs. Acta Physiol. Pol., 1979, 30 (2): 319--326. The study was performed on pigs weighting 50 to 75 kg with temporarily isolated loops of one of the three parts of the small intestine: proximal, middle and terminal. For measurement of intestinal secretion the isolated loops were perfused with N-free solutions and the effluent was collected and analyzed. On a protein diet, under conditions of an isotonic osmotic pressure of the solution, secretion of nitrogen compounds in the upper part was twice that in the lower part of the small intestine, 0.97 against 0.48 g N/m/24 h. A hypertonic pressure increased secretion of N into the intestinal lumen. On a protein-free diet secretion of nitrogen compounds was markedly decreased. Soluble N amounted to about 90% of total N and alpha-amino-N to 50 to 79% of soluble N. Free amino acids made up about 65% and protein 33% of the total alpha-amino-N. There was a large proportion of threonine, 6.0--6.3%, and lysine, 8.0--8.7% in the total amino acids secreted into the intestine."}
{"id": "PMID:463578", "title": "Continuous measurement of flow rate and volume in the nanoliter range.", "content": "A simple transducer which is based on changes of electrical capacity with volume is described. The system accomodates fluid volumes of several microliters and the resolution is below one nanoliter. The response time of the transducer is about 10 ms. The system is applicable for continuous recording of flow rates in renal collecting tubules. Pulsatile flow in the duct of Bellini is demonstrated.", "contents": "Continuous measurement of flow rate and volume in the nanoliter range. A simple transducer which is based on changes of electrical capacity with volume is described. The system accomodates fluid volumes of several microliters and the resolution is below one nanoliter. The response time of the transducer is about 10 ms. The system is applicable for continuous recording of flow rates in renal collecting tubules. Pulsatile flow in the duct of Bellini is demonstrated."}
{"id": "PMID:463579", "title": "Local versus regional cerebral blood flow in the rat at high (hypoxia) and low (phenobarbital anesthesia) flow rates.", "content": "Local cerebral blood flow (CBF) was measured in rats, using an autoradiographic technique with 14C-iodoantipyrine as diffusible tracer, in situations with low, normal and high flow rates (phenobarbital anesthesia, analgesia with 75% N2O, and hypoxia, respectively). A comparison of the results with previous data obtained in conscious rats (Sakurada et al. 1978) demonstrates that 75% N2O moderately reduces local CBF in some, but not all, cortical and subcortical areas, that phenobarbital anesthesia reduces local CBF to between 30 and 65% of (conscious) control, and that pronounced hypoxia (arterial P02 about 25 mmHg) increases local CBF 3- to 4-fold. A comparison of the values obtained for cortical structures with those previously measured with a technique based on the Fick principle shows that the autoradiographic technique gives similar values at low and normal flow rates but that it moderately underestimates CBF at high flow rates, probably due to diffusion limitation.", "contents": "Local versus regional cerebral blood flow in the rat at high (hypoxia) and low (phenobarbital anesthesia) flow rates. Local cerebral blood flow (CBF) was measured in rats, using an autoradiographic technique with 14C-iodoantipyrine as diffusible tracer, in situations with low, normal and high flow rates (phenobarbital anesthesia, analgesia with 75% N2O, and hypoxia, respectively). A comparison of the results with previous data obtained in conscious rats (Sakurada et al. 1978) demonstrates that 75% N2O moderately reduces local CBF in some, but not all, cortical and subcortical areas, that phenobarbital anesthesia reduces local CBF to between 30 and 65% of (conscious) control, and that pronounced hypoxia (arterial P02 about 25 mmHg) increases local CBF 3- to 4-fold. A comparison of the values obtained for cortical structures with those previously measured with a technique based on the Fick principle shows that the autoradiographic technique gives similar values at low and normal flow rates but that it moderately underestimates CBF at high flow rates, probably due to diffusion limitation."}
{"id": "PMID:463569", "title": "Post-exercise changes in muscle glycogen content and blood metabolites in the dogs treated with triiodothyronine.", "content": "Post-exercise changes in muscle glycogen content and blood metabolites in the dogs treated with triiodothyronine. Acta Physiol. Pol., 1979, 30 (2) : 241--252. Prolonged T3-treatment markedly affected muscle glycogen content which was significantly lower than in controls both under resting conditions and after prolonged exercise. The post-exercise rate of the muscle glycogen resynthesis was inhibited in comparison with controls. Glucose (about 35 g per animal) infused to these dogs after cessation of exercise was able to bring about the rate of glycogen resynthesis to control values, but only during the first few hours of the recovery period. The present investigations confirmed that an excess of thyroid hormones elevates the lipolytic and lactacidemic responses to physical effort, but the pattern of the post-exercise changes in blood FAA and LA levels was similar in both groups. No differences between the control and T3-treated animals were found in the levels of blood catecholamines and insulin before, during and after exercise. Blood glucose level was significantly higher in T3-treated dogs at rest and markedly lower at the end of exercise in comparison with controls. The pattern of changes in BG level during the post-exercise period was similar in both animal groups.", "contents": "Post-exercise changes in muscle glycogen content and blood metabolites in the dogs treated with triiodothyronine. Post-exercise changes in muscle glycogen content and blood metabolites in the dogs treated with triiodothyronine. Acta Physiol. Pol., 1979, 30 (2) : 241--252. Prolonged T3-treatment markedly affected muscle glycogen content which was significantly lower than in controls both under resting conditions and after prolonged exercise. The post-exercise rate of the muscle glycogen resynthesis was inhibited in comparison with controls. Glucose (about 35 g per animal) infused to these dogs after cessation of exercise was able to bring about the rate of glycogen resynthesis to control values, but only during the first few hours of the recovery period. The present investigations confirmed that an excess of thyroid hormones elevates the lipolytic and lactacidemic responses to physical effort, but the pattern of the post-exercise changes in blood FAA and LA levels was similar in both groups. No differences between the control and T3-treated animals were found in the levels of blood catecholamines and insulin before, during and after exercise. Blood glucose level was significantly higher in T3-treated dogs at rest and markedly lower at the end of exercise in comparison with controls. The pattern of changes in BG level during the post-exercise period was similar in both animal groups."}
{"id": "PMID:463580", "title": "The effect of vasodilatation and sympathetic nerve activation on net water absorption in the cat's small intestine.", "content": "The rate of net water uptake from the feline small intestine has been investigated during control conditions, during graded infusions of the vasodilator drug isopropylnoradrenaline, and during electrical stimulation of the regional sympathetic nerve fibres to the gut. Net water absorption rate was largely unaffected by intestinal vasodilatation. The fraction of the absorbate transported via the lymphatics remained also constrant at 20-40% of the total absorption regardless of blood flow rate. Stimulating the sympathetic nerve fibres to the small intestine increased, however, net water absorption rate. The increase was particularly pronounced when blood pressure was kept constant during the period of stimulation. The absorption rate was on an average almost doubled at a stimulation frequency of 8 Hz during constant pressure conditions. The mechanism(s) explaining this nervous control of water absorption are tentatively discussed.", "contents": "The effect of vasodilatation and sympathetic nerve activation on net water absorption in the cat's small intestine. The rate of net water uptake from the feline small intestine has been investigated during control conditions, during graded infusions of the vasodilator drug isopropylnoradrenaline, and during electrical stimulation of the regional sympathetic nerve fibres to the gut. Net water absorption rate was largely unaffected by intestinal vasodilatation. The fraction of the absorbate transported via the lymphatics remained also constrant at 20-40% of the total absorption regardless of blood flow rate. Stimulating the sympathetic nerve fibres to the small intestine increased, however, net water absorption rate. The increase was particularly pronounced when blood pressure was kept constant during the period of stimulation. The absorption rate was on an average almost doubled at a stimulation frequency of 8 Hz during constant pressure conditions. The mechanism(s) explaining this nervous control of water absorption are tentatively discussed."}
{"id": "PMID:463570", "title": "Changes in blood vasotocin level in response to uterine stimulation in the hen.", "content": "Changes in blood vasotocin level in response to uterine stimulation in the hen. Acta Physiol. Pol. 1979, 30 (2): 267--272. The blood vasotocin level after uterine and vaginal stimulation was determined by bioassay, on the isolated frog bladder. It was found that uterine distension causes a 2--3 fold increase in the blood vasotocin level compared to that before distension. Vaginal distension was without any effect on the blood vasotocon level.", "contents": "Changes in blood vasotocin level in response to uterine stimulation in the hen. Changes in blood vasotocin level in response to uterine stimulation in the hen. Acta Physiol. Pol. 1979, 30 (2): 267--272. The blood vasotocin level after uterine and vaginal stimulation was determined by bioassay, on the isolated frog bladder. It was found that uterine distension causes a 2--3 fold increase in the blood vasotocin level compared to that before distension. Vaginal distension was without any effect on the blood vasotocon level."}
{"id": "PMID:463581", "title": "Renin, aldosterone and cortisol during ethanol intoxication and hangover.", "content": "The effect of ethanol intoxication and hangover on plasma renin activity (PRA), plasma aldosterone (PA) and plasma cortisol (PC) concentrations was studied in 7 healthy supine men in controlled clinical conditions during 18 h beginning at 6 p.m. Large individual variation was observed in the response of PRA, PA and PC to ethanol. Following ethanol, stimulation of PRA was observed at the 14th and the 16th hour (P less than 0.05), of PA at the 4th and the 6th hour (P less than 0.01 and P less than 0.05, respectively) and of PC at the 4th and the 14th hour (P less than 0.01 and P less than 0.05, respectively). Ethanol ingestion suppressed PC during the first hour (P less than 0.02). Water ingestion at 8 a.m. suppressed PA between the 14th and the 16th hour (8-10 a.m.) in control and ethanol experiment (P less than 0.01 and P less than 0.005, respectively). There was a dissociation between PRA and PA, but intra-individually PRA and PA correlated fairly or well. Plasma arginine vasopressin (AVP) and PC were also significantly correlated. The results suggest that changes in PA and PC as well as the dissociation of PRA and PA after ethanol ingestion might be partly related to dehydration and to the increased secretion of hypothalamic and pituitary hormones as well as to sodium and potassium balance. There was a biphasic effect of ethanol, including an inital suppression of PC and a subsequent increase of PC, PRA and PA. Upright posture appears to exaggerate the stimulating effect of ethanol on PRA, PA and PC.", "contents": "Renin, aldosterone and cortisol during ethanol intoxication and hangover. The effect of ethanol intoxication and hangover on plasma renin activity (PRA), plasma aldosterone (PA) and plasma cortisol (PC) concentrations was studied in 7 healthy supine men in controlled clinical conditions during 18 h beginning at 6 p.m. Large individual variation was observed in the response of PRA, PA and PC to ethanol. Following ethanol, stimulation of PRA was observed at the 14th and the 16th hour (P less than 0.05), of PA at the 4th and the 6th hour (P less than 0.01 and P less than 0.05, respectively) and of PC at the 4th and the 14th hour (P less than 0.01 and P less than 0.05, respectively). Ethanol ingestion suppressed PC during the first hour (P less than 0.02). Water ingestion at 8 a.m. suppressed PA between the 14th and the 16th hour (8-10 a.m.) in control and ethanol experiment (P less than 0.01 and P less than 0.005, respectively). There was a dissociation between PRA and PA, but intra-individually PRA and PA correlated fairly or well. Plasma arginine vasopressin (AVP) and PC were also significantly correlated. The results suggest that changes in PA and PC as well as the dissociation of PRA and PA after ethanol ingestion might be partly related to dehydration and to the increased secretion of hypothalamic and pituitary hormones as well as to sodium and potassium balance. There was a biphasic effect of ethanol, including an inital suppression of PC and a subsequent increase of PC, PRA and PA. Upright posture appears to exaggerate the stimulating effect of ethanol on PRA, PA and PC."}
{"id": "PMID:463584", "title": "A clinical study of 44 patients with juvenile amaurotic family idiocy.", "content": "The material presented comprises 44 patients with juvenile amaurotic family idiocy. The disease is distinguished from other types of gangliosidoses, and earlier clinical descriptions are reviewed. The median age of the patients at the onset of the diseases was 5.8 years and median duration of life was 18.8 years. It appears probable that the disease takes two different courses. Besides the earlier described accompanying phenomena, dystonic attack with oculogyre crises have been found in some patients, as well as extreme cases of bradycardia. In 77% of the patients psychotic manifestations have been found, chiefly in the form of frightening visual hallucinations. The pathogenesis and clinical observations of the psychoses are discussed and environmental factors are pointed out as contributing to the pathogenesis of the psychoses.", "contents": "A clinical study of 44 patients with juvenile amaurotic family idiocy. The material presented comprises 44 patients with juvenile amaurotic family idiocy. The disease is distinguished from other types of gangliosidoses, and earlier clinical descriptions are reviewed. The median age of the patients at the onset of the diseases was 5.8 years and median duration of life was 18.8 years. It appears probable that the disease takes two different courses. Besides the earlier described accompanying phenomena, dystonic attack with oculogyre crises have been found in some patients, as well as extreme cases of bradycardia. In 77% of the patients psychotic manifestations have been found, chiefly in the form of frightening visual hallucinations. The pathogenesis and clinical observations of the psychoses are discussed and environmental factors are pointed out as contributing to the pathogenesis of the psychoses."}
{"id": "PMID:463585", "title": "Depression or asthenia related to metabolic disturbances in obese patients after intestinal bypass surgery.", "content": "This study was an attempt to compare psychological and biological variables in 43 obese patients after intestinal bypass surgery. The difficulties in expressing the psychological variables quantitatively are discussed on the basis of the concept of transferability. By use of an expanded version of the Beck Depression Inventory and the Marke-Nyman Temperament Scale we could demonstrate that items concerning asthenia (self-dislike, irritability, work retardation, insomnia, fatigability, somatic preoccupation about aches and pains, loss of libido, headache, vertigo, palpitations, dryness of the mouth, thirst or increased liquid intake) had, when summed up, a score distribution indicating bimodality. The asthenic group of patients (n = 19) when compared with the non-asthenic patients (n = 24) showed metabolic deficiencies related to the vitamin D complex with no response to oral vitamin D3 administration measured by plasma levels of 25-hydroxyvitamin D3. The lack of response was associated with low calcium excretion in the urine, higher plasma alkaline phosphatase, and a tendency to higher blood levels of parathyroid hormone.", "contents": "Depression or asthenia related to metabolic disturbances in obese patients after intestinal bypass surgery. This study was an attempt to compare psychological and biological variables in 43 obese patients after intestinal bypass surgery. The difficulties in expressing the psychological variables quantitatively are discussed on the basis of the concept of transferability. By use of an expanded version of the Beck Depression Inventory and the Marke-Nyman Temperament Scale we could demonstrate that items concerning asthenia (self-dislike, irritability, work retardation, insomnia, fatigability, somatic preoccupation about aches and pains, loss of libido, headache, vertigo, palpitations, dryness of the mouth, thirst or increased liquid intake) had, when summed up, a score distribution indicating bimodality. The asthenic group of patients (n = 19) when compared with the non-asthenic patients (n = 24) showed metabolic deficiencies related to the vitamin D complex with no response to oral vitamin D3 administration measured by plasma levels of 25-hydroxyvitamin D3. The lack of response was associated with low calcium excretion in the urine, higher plasma alkaline phosphatase, and a tendency to higher blood levels of parathyroid hormone."}
{"id": "PMID:463586", "title": "Video-analytic study of manual kinesics and its lateralization in the course of treatment of depressive syndromes.", "content": "Video-taped interviews of 47, predominantly endogenous depressive in-patients, recorded on days 0 and 21 of pharmacotherapy were analysed with respect to frequency, duration and side-preference of defined hand movements. The data were related to clinical improvement or deterioration as assessed by judgement of the syndromal aspects: retardation and agitation. 1. Above all, improvement is marked by a decrease of Continuous body-focused movements (C) and an increase of Object-focused movements (O) of the speech primacy type (OS)-deterioration by an increase of C and decrease of OS. 2. The degree of retardation corresponds to O, the degree of agitation, above all to C. 3. O show no side-preference with a higher degree of retardation or agitation respectively, but a marked right perference with a lower degree of retardation or agitation, Discrete body touching (D) shows a left preference only, with a higher degree of retardation. A comparison of sidedness of O between interviews 1 and 2 shows no lateralization trend, either in the improvement or the deterioration group. In contrast, D changed from left weightiness to symmetry, coinciding with improvement but not with deterioration, With reference to the latter findings we discuss the possibility of a particular mode of cerebral lateralization predisposing to endogenous depression.", "contents": "Video-analytic study of manual kinesics and its lateralization in the course of treatment of depressive syndromes. Video-taped interviews of 47, predominantly endogenous depressive in-patients, recorded on days 0 and 21 of pharmacotherapy were analysed with respect to frequency, duration and side-preference of defined hand movements. The data were related to clinical improvement or deterioration as assessed by judgement of the syndromal aspects: retardation and agitation. 1. Above all, improvement is marked by a decrease of Continuous body-focused movements (C) and an increase of Object-focused movements (O) of the speech primacy type (OS)-deterioration by an increase of C and decrease of OS. 2. The degree of retardation corresponds to O, the degree of agitation, above all to C. 3. O show no side-preference with a higher degree of retardation or agitation respectively, but a marked right perference with a lower degree of retardation or agitation, Discrete body touching (D) shows a left preference only, with a higher degree of retardation. A comparison of sidedness of O between interviews 1 and 2 shows no lateralization trend, either in the improvement or the deterioration group. In contrast, D changed from left weightiness to symmetry, coinciding with improvement but not with deterioration, With reference to the latter findings we discuss the possibility of a particular mode of cerebral lateralization predisposing to endogenous depression."}
{"id": "PMID:463587", "title": "Alcoholism and antisocial personality.", "content": "The picture of alcoholism in criminals with an antisocial personality was compared with criminals with other personality disorders. Fewer of those who had an antisocial personality were physically dependent on alcohol than those with other personality disorders. They considered their alcoholism to be a problem and behaved as if it were only rare. However, they had a strong tendency to behave abnormally under the influence of alcohol over and over again. In these respects the situation among those who were below the age of 20 was similar. To a great extent, the fathers of those who had an antisocial personality had also had a tendency towards abnormal behavior under the influence of alcohol. This was not so often found in the control group.", "contents": "Alcoholism and antisocial personality. The picture of alcoholism in criminals with an antisocial personality was compared with criminals with other personality disorders. Fewer of those who had an antisocial personality were physically dependent on alcohol than those with other personality disorders. They considered their alcoholism to be a problem and behaved as if it were only rare. However, they had a strong tendency to behave abnormally under the influence of alcohol over and over again. In these respects the situation among those who were below the age of 20 was similar. To a great extent, the fathers of those who had an antisocial personality had also had a tendency towards abnormal behavior under the influence of alcohol. This was not so often found in the control group."}
{"id": "PMID:463588", "title": "EEG response to sine wave modulated light in XYY, XXY, and XY men.", "content": "Ten XYY, 14 XXY, and 47 control XY men were located in a birth cohort of non-institutionalized, tall men. Their EEGs were recorded during stimulation with sine were modulated light; 2.5, 5, 10 and 20 Hz were the modulation frequencies. EEGs with and without stimulation were subjected to power spectral analysis. The frequency-specific EEG change due to visual stimulation (i.e. photic driving) was studied. The XYY subjects showed generally lower levels of driving than their XY controls. At stimulation frequencies of 10 and 20 Hz, the EEG driving in the XYY subjects were significantly smaller when measured in the same frequency bands (i.e. 10 and 20 Hz). In addition, stimulation at 10 Hz elicited a significantly smaller response at the third harmonic frequency (i.e. 30 Hz) in the XYY subjects. No consistent differences in EEG driving were detected between the XXY subjects and their XY controls, or between XXY and XYY subjects.", "contents": "EEG response to sine wave modulated light in XYY, XXY, and XY men. Ten XYY, 14 XXY, and 47 control XY men were located in a birth cohort of non-institutionalized, tall men. Their EEGs were recorded during stimulation with sine were modulated light; 2.5, 5, 10 and 20 Hz were the modulation frequencies. EEGs with and without stimulation were subjected to power spectral analysis. The frequency-specific EEG change due to visual stimulation (i.e. photic driving) was studied. The XYY subjects showed generally lower levels of driving than their XY controls. At stimulation frequencies of 10 and 20 Hz, the EEG driving in the XYY subjects were significantly smaller when measured in the same frequency bands (i.e. 10 and 20 Hz). In addition, stimulation at 10 Hz elicited a significantly smaller response at the third harmonic frequency (i.e. 30 Hz) in the XYY subjects. No consistent differences in EEG driving were detected between the XXY subjects and their XY controls, or between XXY and XYY subjects."}
{"id": "PMID:463589", "title": "Weight changes with depot neuroleptic maintenance therapy.", "content": "A prospective study of schizophrenic patients prescribed injectable depot neuroleptic drugs as maintenance therapy showed a clinically significant weight gain in 55% of patients. No significant difference was shown between flupenthixol decanoate and fluphenazine decanoate, nor was a clinically meaningful relationship shown with dose, or the use of anti-parkinsonian drugs. The weight gain continued for at least 2 years following a mental state relapse. It is suggested that the monitoring of weight and giving of appropriate advice on diet are two essential in maintenance therapy of chronic schizophrenia.", "contents": "Weight changes with depot neuroleptic maintenance therapy. A prospective study of schizophrenic patients prescribed injectable depot neuroleptic drugs as maintenance therapy showed a clinically significant weight gain in 55% of patients. No significant difference was shown between flupenthixol decanoate and fluphenazine decanoate, nor was a clinically meaningful relationship shown with dose, or the use of anti-parkinsonian drugs. The weight gain continued for at least 2 years following a mental state relapse. It is suggested that the monitoring of weight and giving of appropriate advice on diet are two essential in maintenance therapy of chronic schizophrenia."}
{"id": "PMID:463590", "title": "Salivary secretion in the affective disorders and schizophrenia.", "content": "Salivary secretion was measured in 54 psychiatric patients comprising four diagnostic groups: schizophrenia, mania, depression and anxiety state. Detailed psychometric assessment was carried out at the time of measurement. Although salivary flow failed to show an association with either diagnostic category or mental state at the time of examination, it was positively correlated with a subjective rating of appetite. The implications of these findings are discussed.", "contents": "Salivary secretion in the affective disorders and schizophrenia. Salivary secretion was measured in 54 psychiatric patients comprising four diagnostic groups: schizophrenia, mania, depression and anxiety state. Detailed psychometric assessment was carried out at the time of measurement. Although salivary flow failed to show an association with either diagnostic category or mental state at the time of examination, it was positively correlated with a subjective rating of appetite. The implications of these findings are discussed."}
{"id": "PMID:463591", "title": "Etiological relevance of comparisons of high-risk and low-risk groups.", "content": "The prospective study of groups at high risk for pathology is viewed as an increasingly important strategy in research on the etiology and possible prevention of a wide range of disorders. Yet, relatively little attention has been paid to proper and improper etiological interpretations of results based on studies of such groups. The comparison of groups at high risk and low risk for pathology, prior to the development of relevant disturbances in the subjects, cannot provide evidence that a given factor or a characteristic of the groups does or does not contribute to the etiology of the disturbances which will develop in the subjects. For the data to become etiologically relevant, they must be studied as possible antecedents to disturbances identified by following up the subjects. On the other hand, comparisons of high-risk versus low-risk groups are most appropriately interpreted as reflecting the characteristics, correlates and/or consequences of the risk criterion, and such comparisons may thus contribute to better understanding of the true meaning and ramifications of the risk criterion. Considerable caution must be exercised both by high-risk researchers and by observers of high-risk studies in interpreting the significance of results of these studies.", "contents": "Etiological relevance of comparisons of high-risk and low-risk groups. The prospective study of groups at high risk for pathology is viewed as an increasingly important strategy in research on the etiology and possible prevention of a wide range of disorders. Yet, relatively little attention has been paid to proper and improper etiological interpretations of results based on studies of such groups. The comparison of groups at high risk and low risk for pathology, prior to the development of relevant disturbances in the subjects, cannot provide evidence that a given factor or a characteristic of the groups does or does not contribute to the etiology of the disturbances which will develop in the subjects. For the data to become etiologically relevant, they must be studied as possible antecedents to disturbances identified by following up the subjects. On the other hand, comparisons of high-risk versus low-risk groups are most appropriately interpreted as reflecting the characteristics, correlates and/or consequences of the risk criterion, and such comparisons may thus contribute to better understanding of the true meaning and ramifications of the risk criterion. Considerable caution must be exercised both by high-risk researchers and by observers of high-risk studies in interpreting the significance of results of these studies."}
{"id": "PMID:463597", "title": "Human motor activity in decerebrate states and their sequelae.", "content": "The behaviour of the motor activity was investigated in a selected group of neurosurgical patients with diverse cerebral and spinal lesions, as well as in a group of healthy controls. Under standard conditions the EMGs were simultaneously recorded from six muscles, and the time-voltage integral of the muscle action potentials were continuously recorded. In the foregoing work the behaviour of the motor activity in the decerebrate state and its sequelae is described.", "contents": "Human motor activity in decerebrate states and their sequelae. The behaviour of the motor activity was investigated in a selected group of neurosurgical patients with diverse cerebral and spinal lesions, as well as in a group of healthy controls. Under standard conditions the EMGs were simultaneously recorded from six muscles, and the time-voltage integral of the muscle action potentials were continuously recorded. In the foregoing work the behaviour of the motor activity in the decerebrate state and its sequelae is described."}
{"id": "PMID:463598", "title": "Correlative study between computerized transverse scanning and stereoimpedoencephalography in space-occupying lesions of the brain.", "content": "Stereoimpedoencephalography (SIEG) is routinely used by the authors during stereotactic investigations of glial brain tumours prior to stereotactic implantation of isotopes or prior to conventional surgery: the brain tissue impedance is measured along a probe track according to the four electrodes impedance technique. The correlation between the tissue structures and the corresponding impedance values has been studied by comparison with the CATScan data reported on the stereotactic schedules; impedance values are influenced by changes in arterial pCO2 and also by other unknown factors; in order to avoid these uninterpretable changes of the impedance, the recorded values have been normalized with respect to the average impedance along a control track in the normal hemisphere. As a rule, the impedance profile exhibits a \"hole\" at the site of the pathological processes like tumours as well as surrounding oedema, and the delimitation of the pathological area is very similar to that obtained by the CATScan. There is a good linear correlation between the values of normalized impedance and the x-ray absorption as given by the computer listing of the CATScan. Lastly, statistical values of normalized impedances are determined for the main structures of the brain, showing that the tumour tissue has an impedance equal to half the impedance of the normal tissue.", "contents": "Correlative study between computerized transverse scanning and stereoimpedoencephalography in space-occupying lesions of the brain. Stereoimpedoencephalography (SIEG) is routinely used by the authors during stereotactic investigations of glial brain tumours prior to stereotactic implantation of isotopes or prior to conventional surgery: the brain tissue impedance is measured along a probe track according to the four electrodes impedance technique. The correlation between the tissue structures and the corresponding impedance values has been studied by comparison with the CATScan data reported on the stereotactic schedules; impedance values are influenced by changes in arterial pCO2 and also by other unknown factors; in order to avoid these uninterpretable changes of the impedance, the recorded values have been normalized with respect to the average impedance along a control track in the normal hemisphere. As a rule, the impedance profile exhibits a \"hole\" at the site of the pathological processes like tumours as well as surrounding oedema, and the delimitation of the pathological area is very similar to that obtained by the CATScan. There is a good linear correlation between the values of normalized impedance and the x-ray absorption as given by the computer listing of the CATScan. Lastly, statistical values of normalized impedances are determined for the main structures of the brain, showing that the tumour tissue has an impedance equal to half the impedance of the normal tissue."}
{"id": "PMID:463599", "title": "Medial frontal epilepsy: histological, histochemical, and ultrastructural study. Preliminary observations.", "content": "The authors have studied the histological, histochemical, and ultrastructural aspects of eight corticectomies of epileptogenic foci in the medial surfaces of frontal lobes. In all cases they were able to see more gliosis and fewer neurons than normal. The shape and number of dendritic spines were thought to be normal with the Golgi-Cox method. The histochemical study showed an increment of activity of Glucose-6-Phosphatase, Glucose-6-Phosphatedehydrogenase, adenosintriphosphatase, and acid phosphatase. The electron microscope showed, in every case, a variety of changes, among which the authors can point to perivascular lipid accumulation, and an increase of lipofuscin bodies in the cytoplasm of pyramidal cells.", "contents": "Medial frontal epilepsy: histological, histochemical, and ultrastructural study. Preliminary observations. The authors have studied the histological, histochemical, and ultrastructural aspects of eight corticectomies of epileptogenic foci in the medial surfaces of frontal lobes. In all cases they were able to see more gliosis and fewer neurons than normal. The shape and number of dendritic spines were thought to be normal with the Golgi-Cox method. The histochemical study showed an increment of activity of Glucose-6-Phosphatase, Glucose-6-Phosphatedehydrogenase, adenosintriphosphatase, and acid phosphatase. The electron microscope showed, in every case, a variety of changes, among which the authors can point to perivascular lipid accumulation, and an increase of lipofuscin bodies in the cytoplasm of pyramidal cells."}
{"id": "PMID:463600", "title": "Acquired hydrocephalus. II. Diagnostic and prognostic value of quantitative isotope ventriculography (QIV), lumbar isotope cisternography (LIC), pneumoencephalography, and continuous intraventricular pressure recording (CIP).", "content": "The diagnostic and prognostic values of quantitative isotope ventriculography (QIV), lumbar isotope cisternography (LIC), pneumoencephalography (PEG), and continuous intraventricular pressure recording (CIP) were assessed on the basis of the clinical course in 160 patients suspected of having acquired hydrocephalus. The diagnostic value of a given method is defined as its reliability in the diagnosis of hydrocephalus. The reliability in the selection of hydrocephalic patients who will improve on shunt operation is designated as the prognostic value of the method concerned. As the diagnoses are based on isotopic procedures, the diagnostic values of these procedures cannot be assessed. QIV is of greater prognostic value than the other methods of examination. The presence of plateau waves in CIP is a rare, but prognostically valuable sign. The prognostic value of B waves is also good, whereas their diagnostic value is slight. Both from diagnostic and prognostic points of view, PEG is of less value is communicating hydrocephalus on account of the many false findings.", "contents": "Acquired hydrocephalus. II. Diagnostic and prognostic value of quantitative isotope ventriculography (QIV), lumbar isotope cisternography (LIC), pneumoencephalography, and continuous intraventricular pressure recording (CIP). The diagnostic and prognostic values of quantitative isotope ventriculography (QIV), lumbar isotope cisternography (LIC), pneumoencephalography (PEG), and continuous intraventricular pressure recording (CIP) were assessed on the basis of the clinical course in 160 patients suspected of having acquired hydrocephalus. The diagnostic value of a given method is defined as its reliability in the diagnosis of hydrocephalus. The reliability in the selection of hydrocephalic patients who will improve on shunt operation is designated as the prognostic value of the method concerned. As the diagnoses are based on isotopic procedures, the diagnostic values of these procedures cannot be assessed. QIV is of greater prognostic value than the other methods of examination. The presence of plateau waves in CIP is a rare, but prognostically valuable sign. The prognostic value of B waves is also good, whereas their diagnostic value is slight. Both from diagnostic and prognostic points of view, PEG is of less value is communicating hydrocephalus on account of the many false findings."}
{"id": "PMID:463601", "title": "Chronic implanting of electrodes in the cerebellar vermis of the cat. Morphological findings.", "content": "The morphological modifications of the cerebellar cortex have been evaluated in eight cats with chronic electrodes implanted in the anterior vermis. In a control animal a silastic lamina without electrodes was implanted. Four animals received intermittent stimulations up to 11 hours in one case and 100 hours in the other 3. The stimulation was carried out with pulses of 0.5 msc, 200 cps, and a charge per phase of 0.68 muc. Microscopic examination of the cerebellum showed that the experimental implanting of prostheses of the cerebellar vermis produces tissue lesions consisting of fibrous reaction, imprinting of the cortex, gliosis, and loss of Purkinje cells. These lesions are independent of the electric stimulation, and seem to be related to the pressure of foreign material against the cortex.", "contents": "Chronic implanting of electrodes in the cerebellar vermis of the cat. Morphological findings. The morphological modifications of the cerebellar cortex have been evaluated in eight cats with chronic electrodes implanted in the anterior vermis. In a control animal a silastic lamina without electrodes was implanted. Four animals received intermittent stimulations up to 11 hours in one case and 100 hours in the other 3. The stimulation was carried out with pulses of 0.5 msc, 200 cps, and a charge per phase of 0.68 muc. Microscopic examination of the cerebellum showed that the experimental implanting of prostheses of the cerebellar vermis produces tissue lesions consisting of fibrous reaction, imprinting of the cortex, gliosis, and loss of Purkinje cells. These lesions are independent of the electric stimulation, and seem to be related to the pressure of foreign material against the cortex."}
{"id": "PMID:463602", "title": "Negative exploration for lumbar disc prolapse--findings and possible causes.", "content": "32 cases of negative exploration in a joint follow-up series of 3,032 lumbar disc operations are described. Data from 15 hospitals were analyzed by means of questionnaires and computer-aided analysis. Detailed information is given about pre- as well as intraoperative findings. The causes of negative lumbar disc exploration can be divided into two groups. 1. Genuine misdiagnosis where other causes are responsible for the symptoms, and 2. poor technique where despite genuine disc prolapse no evidence was found at operation. The most important causes in the first group were osteochondrotic compression, distended epidural veins, and root tension. The results are less favourable in these patients than in the overall series but, compared to the other complicated courses, the results were markedly better. A review of the literature is given, the possible causes for negative explorations are discussed, and a directive is established for the surgical procedure in cases where an expected prolapse is not found at operation.", "contents": "Negative exploration for lumbar disc prolapse--findings and possible causes. 32 cases of negative exploration in a joint follow-up series of 3,032 lumbar disc operations are described. Data from 15 hospitals were analyzed by means of questionnaires and computer-aided analysis. Detailed information is given about pre- as well as intraoperative findings. The causes of negative lumbar disc exploration can be divided into two groups. 1. Genuine misdiagnosis where other causes are responsible for the symptoms, and 2. poor technique where despite genuine disc prolapse no evidence was found at operation. The most important causes in the first group were osteochondrotic compression, distended epidural veins, and root tension. The results are less favourable in these patients than in the overall series but, compared to the other complicated courses, the results were markedly better. A review of the literature is given, the possible causes for negative explorations are discussed, and a directive is established for the surgical procedure in cases where an expected prolapse is not found at operation."}
{"id": "PMID:463603", "title": "Bladder neuropathy in lumbar disc disease.", "content": "Disturbances of bladder, function often occur in cases of sciatica due to intervertebral disc lesions, although other clinical signs of sacral root compression are absent. In 32 patients, operated upon because of lumbar disc diseases without cauda equina syndrome, micturition symptoms were registered, and bladder function was investigated. Intravesical pressure/volume relations (water cystometry) and simultaneous intravesical pressure/urinary flow relations (urodynamic examination) were recorded preoperatively together with anal sphincter surface EMG. In almost half of the patients cystometry revealed bladder hyposensibility (late first sensation), and a quarter voided mainly by straining (detrusor paresis). These findings were unrelated to the neurosurgical pathology. Pronounced micturition symptoms or abnormal preoperative urodynamic examinations indicated evaluation two months after lumbar disc operation. Two-thirds tended towards recovery, one third remained unchanged.", "contents": "Bladder neuropathy in lumbar disc disease. Disturbances of bladder, function often occur in cases of sciatica due to intervertebral disc lesions, although other clinical signs of sacral root compression are absent. In 32 patients, operated upon because of lumbar disc diseases without cauda equina syndrome, micturition symptoms were registered, and bladder function was investigated. Intravesical pressure/volume relations (water cystometry) and simultaneous intravesical pressure/urinary flow relations (urodynamic examination) were recorded preoperatively together with anal sphincter surface EMG. In almost half of the patients cystometry revealed bladder hyposensibility (late first sensation), and a quarter voided mainly by straining (detrusor paresis). These findings were unrelated to the neurosurgical pathology. Pronounced micturition symptoms or abnormal preoperative urodynamic examinations indicated evaluation two months after lumbar disc operation. Two-thirds tended towards recovery, one third remained unchanged."}
{"id": "PMID:463605", "title": "Terminal ventriculostomy in syringomyelia.", "content": "The authors report their experiences with two patients suffering from syringomyelia on whom terminal ventriculostomies were carried out. The good results which can be obtained if the patient is in a relatively good neurological condition are pointed out. The principle of terminal ventriculostomy is based on the assumption that syringomyelia is hydromyelia that has become symptomatic; with this in mind, it seems reasonable to relieve the patient's symptoms by draining the distended central canal at the lowest possible level. The father of the operation is Dr. Gardner, who has thoroughly studied syringomyelia, and proved that syringomyelia is not only an active hydromyelia but, that a \"non-communicating\" syringomyelia is a non-existent entity (1976, 1977).", "contents": "Terminal ventriculostomy in syringomyelia. The authors report their experiences with two patients suffering from syringomyelia on whom terminal ventriculostomies were carried out. The good results which can be obtained if the patient is in a relatively good neurological condition are pointed out. The principle of terminal ventriculostomy is based on the assumption that syringomyelia is hydromyelia that has become symptomatic; with this in mind, it seems reasonable to relieve the patient's symptoms by draining the distended central canal at the lowest possible level. The father of the operation is Dr. Gardner, who has thoroughly studied syringomyelia, and proved that syringomyelia is not only an active hydromyelia but, that a \"non-communicating\" syringomyelia is a non-existent entity (1976, 1977)."}
{"id": "PMID:463612", "title": "Effects of follicular development on the ability of cultured porcine granulosa cells to convert androgens to estrogens.", "content": "Porcine granulosa cells from different stages of follicular development were examined for their ability to convert androgens to estrogens and their ability to secrete progesterone. Granulosa cells from all stages of follicular development can convert androgens to estrogens, and the addition of exogenous androgen is required for estrogen secretion. Granulosa cells obtained from medium and large follicles have a greater capacity to convert androgens to estrogens than do granulosa cells obtained from small follicles. The addition of FSH brought about an increase in estrogen secretion in the presence of androgen only in granulosa cells from large follicles. LH attenuated estrogen secretion in granulosa cells from medium follicles treated with testosterone. Granulosa cells from medium follicles were unable to secrete estrogen from days two to four irrespective of treatment. Androgens augment FSH stimulated progesterone secretion in granulosa cells from medium follicles from days two to four, and the addition of follicular fluid from small follicles stimulates progesterone secretion in the presence of FSH and androgens.", "contents": "Effects of follicular development on the ability of cultured porcine granulosa cells to convert androgens to estrogens. Porcine granulosa cells from different stages of follicular development were examined for their ability to convert androgens to estrogens and their ability to secrete progesterone. Granulosa cells from all stages of follicular development can convert androgens to estrogens, and the addition of exogenous androgen is required for estrogen secretion. Granulosa cells obtained from medium and large follicles have a greater capacity to convert androgens to estrogens than do granulosa cells obtained from small follicles. The addition of FSH brought about an increase in estrogen secretion in the presence of androgen only in granulosa cells from large follicles. LH attenuated estrogen secretion in granulosa cells from medium follicles treated with testosterone. Granulosa cells from medium follicles were unable to secrete estrogen from days two to four irrespective of treatment. Androgens augment FSH stimulated progesterone secretion in granulosa cells from medium follicles from days two to four, and the addition of follicular fluid from small follicles stimulates progesterone secretion in the presence of FSH and androgens."}
{"id": "PMID:463613", "title": "Testosterone synthesis by chicken follicular cells.", "content": "The roles of theca and of granulosa cells in the synthesis of testosterone (T) in ovarian follicle of hens have been studied. Theca cells alone synthesized no progesterone (P), and no or only negligible amounts of T. Granulosa cells, in contrast, without stimulation produced large amounts of P and small amounts of T. When incubated with ovine gonadotrophins, theca cells showed greater response than granulosa cells in synthesizing more T (2- to 2.5-fold increase). Combination of the two follicular cell types decreased synthesis of P, but increased that of T (9- to 17-fold). When 0.1 microgram of ovine luteinizing hormone (oLH) was added to the combined cells, T concentrations increased most dramatically, a 83-fold increase was seen after 12 h of incubation. Theca cells incubated with exogenous P also produced large amounts of T. These results indicated that in hens, theca cells may be the major site of follicular T synthesis, however, theca cells alone cannot synthesize T unless granulosa cells or P were added. It is suggested that granulosa cells may use P as a precursor, and that P is converted to T in the theca cells.", "contents": "Testosterone synthesis by chicken follicular cells. The roles of theca and of granulosa cells in the synthesis of testosterone (T) in ovarian follicle of hens have been studied. Theca cells alone synthesized no progesterone (P), and no or only negligible amounts of T. Granulosa cells, in contrast, without stimulation produced large amounts of P and small amounts of T. When incubated with ovine gonadotrophins, theca cells showed greater response than granulosa cells in synthesizing more T (2- to 2.5-fold increase). Combination of the two follicular cell types decreased synthesis of P, but increased that of T (9- to 17-fold). When 0.1 microgram of ovine luteinizing hormone (oLH) was added to the combined cells, T concentrations increased most dramatically, a 83-fold increase was seen after 12 h of incubation. Theca cells incubated with exogenous P also produced large amounts of T. These results indicated that in hens, theca cells may be the major site of follicular T synthesis, however, theca cells alone cannot synthesize T unless granulosa cells or P were added. It is suggested that granulosa cells may use P as a precursor, and that P is converted to T in the theca cells."}
{"id": "PMID:463614", "title": "Modulation of thecal progesterone secretion by estradiol-17 beta.", "content": "The effects of estradiol-17 beta on progesterone secretion by isolated bovine theca interna were studied. Preparations of theca interna were obtained from preovulatory follicles of Holstein heifers (n = 5) prior to the endogenous LH surge and cultured for 3 days in a defined medium containing various concentrations of estradiol (0, 0.001, 0.01, 0.1, 1, or 10 microgram/ml). Secretion of progesterone by control theca was low during the first 24 h of culture and increased steadily during the subsequent 48 h. The lowest concentration of estradiol (0.001 microgram/ml) significantly stimulated thecal progesterone production, while higher concentrations (0.1, 1, and 10 microgram/ml) significantly inhibited secretion of progesterone. Estradiol content of the follicular fluid of these preovulatory follicles and several others (n = 13) averaged 1.5 microgram/ml +/- 0.1 (SEM). It is concluded that bovine theca interna is capable of secreting progesterone, that high intrafollicular concentrations of estradiol may inhibit thecal progesterone production in vivo prior to the LH surge, and that estradiol concentration may be be an important modulating factor in the control of follicular progesterone secretion.", "contents": "Modulation of thecal progesterone secretion by estradiol-17 beta. The effects of estradiol-17 beta on progesterone secretion by isolated bovine theca interna were studied. Preparations of theca interna were obtained from preovulatory follicles of Holstein heifers (n = 5) prior to the endogenous LH surge and cultured for 3 days in a defined medium containing various concentrations of estradiol (0, 0.001, 0.01, 0.1, 1, or 10 microgram/ml). Secretion of progesterone by control theca was low during the first 24 h of culture and increased steadily during the subsequent 48 h. The lowest concentration of estradiol (0.001 microgram/ml) significantly stimulated thecal progesterone production, while higher concentrations (0.1, 1, and 10 microgram/ml) significantly inhibited secretion of progesterone. Estradiol content of the follicular fluid of these preovulatory follicles and several others (n = 13) averaged 1.5 microgram/ml +/- 0.1 (SEM). It is concluded that bovine theca interna is capable of secreting progesterone, that high intrafollicular concentrations of estradiol may inhibit thecal progesterone production in vivo prior to the LH surge, and that estradiol concentration may be be an important modulating factor in the control of follicular progesterone secretion."}
{"id": "PMID:463616", "title": "Long term perifusion of rabbit ovarian follicles.", "content": "Preovulatory follicles were dissected from ovaries of estrous rabbits. Four follicles were placed in each chamber of a perifusion system. Medium 199, containing 10% rabbit serum and antibiotics, eluted through the chambers at a rate of 1 ml/10 min. Every 6 h for 36 h, 1 microgram of LH and 50 ng of FSH were injected into each chamber. P4, T and E2 were measured in the medium. P4 exhibited a peak after each of the injections at 6, 12, 18, 24 and 30 h. E2 and T gave major peaks after the injection at 0 time, with minor peaks after the injection at 6 h; thereafter T and E2 secretion gradually decreased to levels below those of baseline.", "contents": "Long term perifusion of rabbit ovarian follicles. Preovulatory follicles were dissected from ovaries of estrous rabbits. Four follicles were placed in each chamber of a perifusion system. Medium 199, containing 10% rabbit serum and antibiotics, eluted through the chambers at a rate of 1 ml/10 min. Every 6 h for 36 h, 1 microgram of LH and 50 ng of FSH were injected into each chamber. P4, T and E2 were measured in the medium. P4 exhibited a peak after each of the injections at 6, 12, 18, 24 and 30 h. E2 and T gave major peaks after the injection at 0 time, with minor peaks after the injection at 6 h; thereafter T and E2 secretion gradually decreased to levels below those of baseline."}
{"id": "PMID:463618", "title": "Action of porcine follicular fluid oocyte maturation inhibitor in vitro: possible role of the cumulus cells.", "content": "To study the mode of action of porcine follicular fluid oocyte maturation inhibitor isolated cumulus-enclosed or mechanically denuded pig oocytes were used. Two types of culture media were employed, a complex containing 15% pig serum (TC199A) and a defined, minimal medium (BMOC). The maturation of cumulus-enclosed oocytes was comparable in the two types of culture media, but only in the complex medium did the cumulus cells remain functional in terms of morphology and progesterone secretion. The low molecular weight portion of follicular fluid partially inhibited oocyte meiosis and cumulus progesterone secretion in 199A. No inhibition of oocyte maturation was seen when follicular fluid was added to BMOC. Since denuded oocytes did not respond to follicular fluid in either culture medium, it is suggested that the cumulus cells may mediate the action of the follicular fluid oocyte maturation inhibitor.", "contents": "Action of porcine follicular fluid oocyte maturation inhibitor in vitro: possible role of the cumulus cells. To study the mode of action of porcine follicular fluid oocyte maturation inhibitor isolated cumulus-enclosed or mechanically denuded pig oocytes were used. Two types of culture media were employed, a complex containing 15% pig serum (TC199A) and a defined, minimal medium (BMOC). The maturation of cumulus-enclosed oocytes was comparable in the two types of culture media, but only in the complex medium did the cumulus cells remain functional in terms of morphology and progesterone secretion. The low molecular weight portion of follicular fluid partially inhibited oocyte meiosis and cumulus progesterone secretion in 199A. No inhibition of oocyte maturation was seen when follicular fluid was added to BMOC. Since denuded oocytes did not respond to follicular fluid in either culture medium, it is suggested that the cumulus cells may mediate the action of the follicular fluid oocyte maturation inhibitor."}
{"id": "PMID:463622", "title": "The influence of follicular fluid on progesterone secretion by porcine granulosa cells in vitro.", "content": "Porcine follicular fluid contains at least two factors which can modify porcine granulosa cell function in vitro. One factor, found primarily in fluid from 1--2 mm follicles inhibits LH stimulated cyclic AMP accumulation, basal and LH stimulated progesterone secretion, and morphological luteinization of granulosa cells from medium sized (3--5 mm) and large (6--12 mm) follicles. Following inhibition by the follicular fluid luteinization inhibitor, incubation with both OH and a phosphodiesterase inhibitor partially restores cyclic AMP accumulation and fully restores progesterone secretion. This suggests that the defect induced by the luteinization inhibitor is primarily an alteration of cyclic nucleotide metabolism. Another factor found primarily in fluid from large preovulatory follicles stimulates basal and gonadotrophin stimulated progesterone secretion by granulosa cells from small antral follicles. We hypothesize that it may be necessary for the fluid within an individual follicle to shift from being inhibitory of luteinization to being permissive or stimulatory for the granulosa cells to mature rather than become atretic.", "contents": "The influence of follicular fluid on progesterone secretion by porcine granulosa cells in vitro. Porcine follicular fluid contains at least two factors which can modify porcine granulosa cell function in vitro. One factor, found primarily in fluid from 1--2 mm follicles inhibits LH stimulated cyclic AMP accumulation, basal and LH stimulated progesterone secretion, and morphological luteinization of granulosa cells from medium sized (3--5 mm) and large (6--12 mm) follicles. Following inhibition by the follicular fluid luteinization inhibitor, incubation with both OH and a phosphodiesterase inhibitor partially restores cyclic AMP accumulation and fully restores progesterone secretion. This suggests that the defect induced by the luteinization inhibitor is primarily an alteration of cyclic nucleotide metabolism. Another factor found primarily in fluid from large preovulatory follicles stimulates basal and gonadotrophin stimulated progesterone secretion by granulosa cells from small antral follicles. We hypothesize that it may be necessary for the fluid within an individual follicle to shift from being inhibitory of luteinization to being permissive or stimulatory for the granulosa cells to mature rather than become atretic."}
{"id": "PMID:463623", "title": "Follicular determinants of corpus luteum function in the human ovary.", "content": "The corpus luteum is a direct continuation of preovulatory follicle development. Growth and differentiation of a follicle to the preovulatory stage depends on certain endocrine changes taking place within the follicle itself (5). These endocrine changes markedly influence the viability, the rate of proliferation, and the biosynthetic potential of granulosa cells. Therefore, the sequence of endocrine events within the developing antral follicle is likely to be of considerable importance for the formation and secretory activity of the corpus luteum. If granulosa cells in developing antral follicles are exposed to certian hormones in the wrong sequence, the follicle may be prevented from developing into a normal corpus luteum. For example, if granulosa cells in antral follicles are not exposed to FSH, they are unable to generate high levels of oestrogen (Table 1). In the absence of oestrogen, granulosa cells do not increase in number. The same end result may also arise if the granulosa cells are exposed prematurely to elevated levels of LH or to high levels of prolactin: LH blocks granulosa cell mitoses even if the cells are exposed to oestrogen (1, 21); high levels of prolactin in antral fluid are associated with low levels of oestrogen and a reduced rate of granulosa cell proliferation. In women, it is likely that a fully functional corpus luteum is formed from a follicle that was exposed to the most favourable sequence of endocrine changes during its antral stages of development.", "contents": "Follicular determinants of corpus luteum function in the human ovary. The corpus luteum is a direct continuation of preovulatory follicle development. Growth and differentiation of a follicle to the preovulatory stage depends on certain endocrine changes taking place within the follicle itself (5). These endocrine changes markedly influence the viability, the rate of proliferation, and the biosynthetic potential of granulosa cells. Therefore, the sequence of endocrine events within the developing antral follicle is likely to be of considerable importance for the formation and secretory activity of the corpus luteum. If granulosa cells in developing antral follicles are exposed to certian hormones in the wrong sequence, the follicle may be prevented from developing into a normal corpus luteum. For example, if granulosa cells in antral follicles are not exposed to FSH, they are unable to generate high levels of oestrogen (Table 1). In the absence of oestrogen, granulosa cells do not increase in number. The same end result may also arise if the granulosa cells are exposed prematurely to elevated levels of LH or to high levels of prolactin: LH blocks granulosa cell mitoses even if the cells are exposed to oestrogen (1, 21); high levels of prolactin in antral fluid are associated with low levels of oestrogen and a reduced rate of granulosa cell proliferation. In women, it is likely that a fully functional corpus luteum is formed from a follicle that was exposed to the most favourable sequence of endocrine changes during its antral stages of development."}
{"id": "PMID:463634", "title": "Effect of oestradiol-17 beta and gonadotrophins on prostaglandin production by pre-ovulatory pig follicles superfused in vitro.", "content": "Pre-ovulatory pig follicles were superfused in vitro with oestradiol, LH or FSH in the presence and absence of indomethacin. Prostaglandin E2 ANd F2 alpha and oestradiol were measured in the same samples of superfusate. LH and FSH caused an increase in levels of both prostaglandins (approximately 2-fold) but the most marked effect was of oestradiol in PGE2 where a 4-fold increase in levels above controls was observed. In all cases indomethacin inhibited the effects. Both gonadotrophins caused an increase in oestradiol production although the response to LH was much slower than to FSH. The results show a possible relationship between FSH, oestradiol and PG synthesis in the pre-ovulatory pig follicle.", "contents": "Effect of oestradiol-17 beta and gonadotrophins on prostaglandin production by pre-ovulatory pig follicles superfused in vitro. Pre-ovulatory pig follicles were superfused in vitro with oestradiol, LH or FSH in the presence and absence of indomethacin. Prostaglandin E2 ANd F2 alpha and oestradiol were measured in the same samples of superfusate. LH and FSH caused an increase in levels of both prostaglandins (approximately 2-fold) but the most marked effect was of oestradiol in PGE2 where a 4-fold increase in levels above controls was observed. In all cases indomethacin inhibited the effects. Both gonadotrophins caused an increase in oestradiol production although the response to LH was much slower than to FSH. The results show a possible relationship between FSH, oestradiol and PG synthesis in the pre-ovulatory pig follicle."}
{"id": "PMID:463642", "title": "Interactions between neuraminidase-treated lymphocytes and liver cells.", "content": "Neuraminidase-treated rat lymphocytes and rat hepatocytes spontaneously aggregate when mixed in vitro. Adhesion between cells is due to stereo-specific interactions between a mammalian hepatic membrane lectin and galactosyl residues which are exposed on the lymphocyte surface after removal of sialic acid residues. The hepatic galactose specific lectin may play a role in the accumulation of recirculating desialylated lymphocytes in the liver.", "contents": "Interactions between neuraminidase-treated lymphocytes and liver cells. Neuraminidase-treated rat lymphocytes and rat hepatocytes spontaneously aggregate when mixed in vitro. Adhesion between cells is due to stereo-specific interactions between a mammalian hepatic membrane lectin and galactosyl residues which are exposed on the lymphocyte surface after removal of sialic acid residues. The hepatic galactose specific lectin may play a role in the accumulation of recirculating desialylated lymphocytes in the liver."}
{"id": "PMID:463646", "title": "Germinal center formation in BSA-tolerant chickens.", "content": "In chickens rendered neonatally tolerant to BSA the germinal center formation was significantly decreased after stimulation with BSA at the age of 3 weeks. At the breakdown of tolerance after the age of 6 weeks the IgG antibody formations recovered before the IgM production. Stimulation of tolerant birds with unrelated antigens resulted in slightly decreased antibody response but the germinal center formation was on the same level as in normal controls.", "contents": "Germinal center formation in BSA-tolerant chickens. In chickens rendered neonatally tolerant to BSA the germinal center formation was significantly decreased after stimulation with BSA at the age of 3 weeks. At the breakdown of tolerance after the age of 6 weeks the IgG antibody formations recovered before the IgM production. Stimulation of tolerant birds with unrelated antigens resulted in slightly decreased antibody response but the germinal center formation was on the same level as in normal controls."}
{"id": "PMID:463647", "title": "Induction of tolerance to DNCB-contact sensitivity in guinea pig fetuses.", "content": "1. Female guinea pigs made tolerant before pregnancy do not transfer tolerance to their offspring. This result favours the concept of cellular mechanism of tolerance. 2. Guinea pig fetuses become tolerant when exposed to the tolerogen in the last two weeks of pregnancy. This is due to active tolerization by the tolerogen passing the placental barrier. 3. Fetuses exposed to the antigen earlier did not develop tolerance. This is due to immunological immaturity rather than to insufficiently developed vascularization. No functional active depot of tolerogen is formed. 4. Guinea pigs become immunologically mature between the 54th and 61st day of fetal live.", "contents": "Induction of tolerance to DNCB-contact sensitivity in guinea pig fetuses. 1. Female guinea pigs made tolerant before pregnancy do not transfer tolerance to their offspring. This result favours the concept of cellular mechanism of tolerance. 2. Guinea pig fetuses become tolerant when exposed to the tolerogen in the last two weeks of pregnancy. This is due to active tolerization by the tolerogen passing the placental barrier. 3. Fetuses exposed to the antigen earlier did not develop tolerance. This is due to immunological immaturity rather than to insufficiently developed vascularization. No functional active depot of tolerogen is formed. 4. Guinea pigs become immunologically mature between the 54th and 61st day of fetal live."}
{"id": "PMID:463661", "title": "In vivo activity of lymphocytes sensitized in vitro by antigen-fed macrophages: inhibition of lymphoma growth.", "content": "In previous studies, we have shown that macrophages fed with radiation leukemia virus could induce primary in vitro sensitization of lymphocytes which could be measured by their cytotoxic activity against target cells. In the present study, we tested the in vivo influence on tumor growth of such lymphocytes. We found that macrophage-mediated sensitized lymphocytes could protect mice against tumor growth if injected into normal recipients four days prior to challenge with lymphoma cells. The protective function of such lymphocytes was not affected by their irradiation, but no protection occurred in sublethally irradiated recipients. This indicated that the sensitized lymphocytes did not inhibit tumor cell growth directly but recruited an effector protective response in the recipient mice. This protective activity was different from the one elicited by lymphocytes which had been sensitized directly against cells carrying antigens cross-reacting with RadLV. The protective activity of the directly sensitized lymphocytes was radiosensitive and was probably mediated by their direct cytotoxic activity against tumor cells.", "contents": "In vivo activity of lymphocytes sensitized in vitro by antigen-fed macrophages: inhibition of lymphoma growth. In previous studies, we have shown that macrophages fed with radiation leukemia virus could induce primary in vitro sensitization of lymphocytes which could be measured by their cytotoxic activity against target cells. In the present study, we tested the in vivo influence on tumor growth of such lymphocytes. We found that macrophage-mediated sensitized lymphocytes could protect mice against tumor growth if injected into normal recipients four days prior to challenge with lymphoma cells. The protective function of such lymphocytes was not affected by their irradiation, but no protection occurred in sublethally irradiated recipients. This indicated that the sensitized lymphocytes did not inhibit tumor cell growth directly but recruited an effector protective response in the recipient mice. This protective activity was different from the one elicited by lymphocytes which had been sensitized directly against cells carrying antigens cross-reacting with RadLV. The protective activity of the directly sensitized lymphocytes was radiosensitive and was probably mediated by their direct cytotoxic activity against tumor cells."}
{"id": "PMID:463663", "title": "Comparison of the ability of eosinophils and neutrophils, and of eosinophils from patients with S. mansoni infection and normal individuals, to mediate in vitro damage to schistosomula of S. mansoni.", "content": "A reliable and reproducible method that produces separate fractions of pure eosinophils and neutrophils from normal peripheral blood was described. The interaction of eosinophils and neutrophils with antibody coated schistosomula was examined in vitro. Neutrophils were highly active in the 51Cr release assay and most formed rosettes with antibody coated red cells, but they adhered poorly to schistosomula and did not kill the organisms. Eosinophils, although they were less active in the 51Cr release assay than neutrophils and few formed rosettes, adhered strongly to schistosomula and, in the presence of antibody, were able to kill organisms. Organisms from patients with S. mansoni infection and eosinophilia were more effecient than eosinophils from normal individuals in their capacity to release 51Cr from labelled somula in the presence of antibody.", "contents": "Comparison of the ability of eosinophils and neutrophils, and of eosinophils from patients with S. mansoni infection and normal individuals, to mediate in vitro damage to schistosomula of S. mansoni. A reliable and reproducible method that produces separate fractions of pure eosinophils and neutrophils from normal peripheral blood was described. The interaction of eosinophils and neutrophils with antibody coated schistosomula was examined in vitro. Neutrophils were highly active in the 51Cr release assay and most formed rosettes with antibody coated red cells, but they adhered poorly to schistosomula and did not kill the organisms. Eosinophils, although they were less active in the 51Cr release assay than neutrophils and few formed rosettes, adhered strongly to schistosomula and, in the presence of antibody, were able to kill organisms. Organisms from patients with S. mansoni infection and eosinophilia were more effecient than eosinophils from normal individuals in their capacity to release 51Cr from labelled somula in the presence of antibody."}
{"id": "PMID:463673", "title": "Peripheral blood lymphocyte subpopulations in multiple myeloma.", "content": "Antisera raised against idiotypic determinants of myeloma proteins and macroglobulins have been used to differentiate peripheral blood lymphocytes populations from individual patients. I.D.-positive lymphocytes not resembling plasma cells have been regularly found in peripheral blood in these diseases. This lymphocyte population is heterogeneous with respect to non-tumor-specific surface markers, such as SRBC-, Fc- and C-receptors. Tumor specific idiotypic determinants will thus allow a more correct recognition of the total tumor cell compartment in these diseases.", "contents": "Peripheral blood lymphocyte subpopulations in multiple myeloma. Antisera raised against idiotypic determinants of myeloma proteins and macroglobulins have been used to differentiate peripheral blood lymphocytes populations from individual patients. I.D.-positive lymphocytes not resembling plasma cells have been regularly found in peripheral blood in these diseases. This lymphocyte population is heterogeneous with respect to non-tumor-specific surface markers, such as SRBC-, Fc- and C-receptors. Tumor specific idiotypic determinants will thus allow a more correct recognition of the total tumor cell compartment in these diseases."}
{"id": "PMID:463675", "title": "Pathophysiology of blood pressure in the elderly.", "content": "Several of the factors responsible for circulatory control are modified in the elderly. Loss of elasticity of the aortic wall produces a widened pulse pressure and a high incidence of systolic hypertension. Attempts to normalize this may be associated with disabling diastolic hypotension. Arterial baroreceptor sensitivity and responsiveness of the renin-angiotensin system is reduced. Further, the renal capacity to conserve sodium and water is impaired. All these increase the risks associated with antihypertensive treatment, but do not contra-indicate such treatment.", "contents": "Pathophysiology of blood pressure in the elderly. Several of the factors responsible for circulatory control are modified in the elderly. Loss of elasticity of the aortic wall produces a widened pulse pressure and a high incidence of systolic hypertension. Attempts to normalize this may be associated with disabling diastolic hypotension. Arterial baroreceptor sensitivity and responsiveness of the renin-angiotensin system is reduced. Further, the renal capacity to conserve sodium and water is impaired. All these increase the risks associated with antihypertensive treatment, but do not contra-indicate such treatment."}
{"id": "PMID:463676", "title": "Should we treat hypertension in the elderly?", "content": "The argument for treating hypertension in old people is essentially that the treatment is beneficial in young people. Direct evidence of benefit in old people is limited. Further evidence is awaited from the European Cooperative Study. Critical examination of the indirect evidence gives limited support to treatment of certain sub-groups of the elderly only. Much present practice appears to be unsound.", "contents": "Should we treat hypertension in the elderly? The argument for treating hypertension in old people is essentially that the treatment is beneficial in young people. Direct evidence of benefit in old people is limited. Further evidence is awaited from the European Cooperative Study. Critical examination of the indirect evidence gives limited support to treatment of certain sub-groups of the elderly only. Much present practice appears to be unsound."}
{"id": "PMID:463677", "title": "Glucose tolerance, plasma insulin levels and insulin sensitivity in elderly patients.", "content": "The oral glucose tolerance test (OGTT) and the intravenous glucose tolerance test (ivGTT) were carried out in 23 elderly patients (mean age 79 years) and in 14 healthy young adults (mean age 28 years). The ivGTT was followed immediately by a second injection of glucose, this time with insulin added. The percentage rate constants for the disappearance of glucose alone (KG, % min-1) or with added insulin (KG+I, % min-1) were calculated. Seventeen of the elderly patients had a blood glucose value at 120 min in the OGTT greater than or equal to 7.7 mmol/1 (140 mg/100 ml) or had KG less than or equal to 1% min-1 and 13 were 'diabetic' by both criteria. This was associated with a sluggish pattern of insulin release and with diminished insulin sensitivity (KG+I). Since these changes could reflect impaired inhibition of hepatic glucose output, a preliminary attempt was made to assess gluconeogenic capacity. After the injection of substrate levels of L-alanine, plasma levels of glucose and alanine both rose but fell more slowly in the elderly than in young adults, suggesting impaired transmembrane transport of both substances in old people. After injection of alanine, plasma insulin rose to a lesser extent and glucagon to a greater extent in the elderly than in young adults.", "contents": "Glucose tolerance, plasma insulin levels and insulin sensitivity in elderly patients. The oral glucose tolerance test (OGTT) and the intravenous glucose tolerance test (ivGTT) were carried out in 23 elderly patients (mean age 79 years) and in 14 healthy young adults (mean age 28 years). The ivGTT was followed immediately by a second injection of glucose, this time with insulin added. The percentage rate constants for the disappearance of glucose alone (KG, % min-1) or with added insulin (KG+I, % min-1) were calculated. Seventeen of the elderly patients had a blood glucose value at 120 min in the OGTT greater than or equal to 7.7 mmol/1 (140 mg/100 ml) or had KG less than or equal to 1% min-1 and 13 were 'diabetic' by both criteria. This was associated with a sluggish pattern of insulin release and with diminished insulin sensitivity (KG+I). Since these changes could reflect impaired inhibition of hepatic glucose output, a preliminary attempt was made to assess gluconeogenic capacity. After the injection of substrate levels of L-alanine, plasma levels of glucose and alanine both rose but fell more slowly in the elderly than in young adults, suggesting impaired transmembrane transport of both substances in old people. After injection of alanine, plasma insulin rose to a lesser extent and glucagon to a greater extent in the elderly than in young adults."}
{"id": "PMID:463678", "title": "Motor complications of herpes zoster.", "content": "Motor involvement is uncommon in herpes zoster. Of 14 subjects observed over an arbitrary period of five years, nine had flaccid limb paralyses, involving the upper limb in eight patients. Recovery was not always complete and occurred over a period of nine weeks to four years.", "contents": "Motor complications of herpes zoster. Motor involvement is uncommon in herpes zoster. Of 14 subjects observed over an arbitrary period of five years, nine had flaccid limb paralyses, involving the upper limb in eight patients. Recovery was not always complete and occurred over a period of nine weeks to four years."}
{"id": "PMID:463679", "title": "The prevalence, severity and factors associated with urinary incontinence in a random sample of the elderly.", "content": "A study to determine the prevalence of urinary incontinence in a random sample of a total elderly community is described. The prevalence of incontinence in women aged 65 years or more was found to be 17% and in men 11%. The findings show that the prevalence of incontinence is particularly high among residents of old people's homes and geriatric hospitals, but that the majority of cases occur within the general community. The prevalence increases with age in both sexes; associations with a history of cerebrovascular disease, certain surgical procedures, multiple hospital admissions and drug usage are described. The findings of a follow-up study suggest that, although the disorder is long-standing and severe in a proportion of subjects, it is transient in approximately a third of all elderly subjects with the condition.", "contents": "The prevalence, severity and factors associated with urinary incontinence in a random sample of the elderly. A study to determine the prevalence of urinary incontinence in a random sample of a total elderly community is described. The prevalence of incontinence in women aged 65 years or more was found to be 17% and in men 11%. The findings show that the prevalence of incontinence is particularly high among residents of old people's homes and geriatric hospitals, but that the majority of cases occur within the general community. The prevalence increases with age in both sexes; associations with a history of cerebrovascular disease, certain surgical procedures, multiple hospital admissions and drug usage are described. The findings of a follow-up study suggest that, although the disorder is long-standing and severe in a proportion of subjects, it is transient in approximately a third of all elderly subjects with the condition."}
{"id": "PMID:463680", "title": "Will drugs help patients with Alzheimer's disease?", "content": "Recent studies have shown a defect in the cholinergic system of the cerebral cortex of patients suffering from nonvascular senile dementia. This observation suggests the possibility of instituting some kind of replacement therapy and trial have been carried out. At this juncture it seem pertinent to ask what lessons can we learn from Parkinson's disease, what is the evidence for a biochemical defect, is this defect relevant and can it be remedied? These questions are considered in turn and suggestions are made about the likely difficulties to be overcome in a search for a treatment of dementia.", "contents": "Will drugs help patients with Alzheimer's disease? Recent studies have shown a defect in the cholinergic system of the cerebral cortex of patients suffering from nonvascular senile dementia. This observation suggests the possibility of instituting some kind of replacement therapy and trial have been carried out. At this juncture it seem pertinent to ask what lessons can we learn from Parkinson's disease, what is the evidence for a biochemical defect, is this defect relevant and can it be remedied? These questions are considered in turn and suggestions are made about the likely difficulties to be overcome in a search for a treatment of dementia."}
{"id": "PMID:463681", "title": "Infectious mononucleosis in the elderly.", "content": "Infectious mononucleosis (IM) was diagnosed in four patients over the age of 50 years. Their age, absence of splenomegaly, lack of significant lymphadenopathy in three and an atypical presentation in one all contributed to a delay in the diagnosis. In two patients, in whom complications occurred, the Paul-Bunnell test was repeatedly negative. Confirmation of the diagnosis was made by the measurement of the Epstein-Barr virus IgM using differential sucrose gradient centrifugation. Because the presentation and clinical features of IM can be misleading in the elderly, we believe that a significant number of cases may go unrecognized.", "contents": "Infectious mononucleosis in the elderly. Infectious mononucleosis (IM) was diagnosed in four patients over the age of 50 years. Their age, absence of splenomegaly, lack of significant lymphadenopathy in three and an atypical presentation in one all contributed to a delay in the diagnosis. In two patients, in whom complications occurred, the Paul-Bunnell test was repeatedly negative. Confirmation of the diagnosis was made by the measurement of the Epstein-Barr virus IgM using differential sucrose gradient centrifugation. Because the presentation and clinical features of IM can be misleading in the elderly, we believe that a significant number of cases may go unrecognized."}
{"id": "PMID:463685", "title": "Vascular pathology in hypertension.", "content": "The pathological changes in blood vessels observed in primary (essential hypertension) are similar to those seen in secondary hypertension due to renal disease or other causes. In benign hypertension, the major changes are in the small arteries and arterioles especially in the kidney. Interlobular arteries exhibit intimal thickening and duplication of the elastic lamina (elastosis) and there is hyaline change in the media of many arterioles. In some respects these changes are an accentuation of vessel ageing. Malignant hypertension usually presents in a younger age group (35--50 years) and is characterized pathologically by fibrous endarteritis in the interlobular arteries of the kidney and fibrinoid necrosis in the walls of a proportion of the efferent glomerular arterioles. Similar vessel changes are seen in other organs but many of the pathological changes in the heart and brain of patients with benign hypertension are related to the accentuation of arterosclerosis. There is an increased mortality from cardiac failure, myocardial infarction, cerebral haemorrhage and subarachnoid haemorrhage due to ruptured berry aneurysms in patients with benign hypertension. Although there is ischaemic damage to the kidneys in benign hypertension, death from renal failure is uncommon. Severe ischaemic damage to renal glomeruli and renal failure does, however, occur in malignant hypertension.", "contents": "Vascular pathology in hypertension. The pathological changes in blood vessels observed in primary (essential hypertension) are similar to those seen in secondary hypertension due to renal disease or other causes. In benign hypertension, the major changes are in the small arteries and arterioles especially in the kidney. Interlobular arteries exhibit intimal thickening and duplication of the elastic lamina (elastosis) and there is hyaline change in the media of many arterioles. In some respects these changes are an accentuation of vessel ageing. Malignant hypertension usually presents in a younger age group (35--50 years) and is characterized pathologically by fibrous endarteritis in the interlobular arteries of the kidney and fibrinoid necrosis in the walls of a proportion of the efferent glomerular arterioles. Similar vessel changes are seen in other organs but many of the pathological changes in the heart and brain of patients with benign hypertension are related to the accentuation of arterosclerosis. There is an increased mortality from cardiac failure, myocardial infarction, cerebral haemorrhage and subarachnoid haemorrhage due to ruptured berry aneurysms in patients with benign hypertension. Although there is ischaemic damage to the kidneys in benign hypertension, death from renal failure is uncommon. Severe ischaemic damage to renal glomeruli and renal failure does, however, occur in malignant hypertension."}
{"id": "PMID:463687", "title": "Synergism between subthreshold doses of phenylbutazone and prednisolone in rat hind-paw carrageenan edema.", "content": "When phenylbutazone (PhB) and prednisolone (PD) or cortisol acetate (HC) were administered together by oral route a synergic inhibitory effect on rat hind-paw carrageenan edema occurred, marked antiphologistic effect being obtained by associating subthreshold doses of drugs. By administering a fixed subthreshold dose of PD with increasing subthreshold doses of PhB, statistically significant phlogosis inhibition was obtained, the degree of which was directly related to the amount of PhB. Finally, the association of near maximal effective doses of PhB and PD gave an inhibition greater than that produced by a maximally effective dose of each of the associated drugs.", "contents": "Synergism between subthreshold doses of phenylbutazone and prednisolone in rat hind-paw carrageenan edema. When phenylbutazone (PhB) and prednisolone (PD) or cortisol acetate (HC) were administered together by oral route a synergic inhibitory effect on rat hind-paw carrageenan edema occurred, marked antiphologistic effect being obtained by associating subthreshold doses of drugs. By administering a fixed subthreshold dose of PD with increasing subthreshold doses of PhB, statistically significant phlogosis inhibition was obtained, the degree of which was directly related to the amount of PhB. Finally, the association of near maximal effective doses of PhB and PD gave an inhibition greater than that produced by a maximally effective dose of each of the associated drugs."}
{"id": "PMID:463688", "title": "Effect of ergometrine on contractile force of guinea-pig isolated heart: antagonism by cimetidine.", "content": "The action of ergometrine on contractile force of guinea-pig isolated heart was investigated and the effect of receptor antagonists on the inotropic effect of ergometrine, isoproterenol, dopamine and histamine was evaluated. Ergometrine was found to have a good dose-dependent inotropic effect and the antagonism of cimetidine toward this action is reported. Experiments carried out in hearts in which a massive depletion of mast-cells histamine was produced are in favour of a direct action of the drug on H2-receptors.", "contents": "Effect of ergometrine on contractile force of guinea-pig isolated heart: antagonism by cimetidine. The action of ergometrine on contractile force of guinea-pig isolated heart was investigated and the effect of receptor antagonists on the inotropic effect of ergometrine, isoproterenol, dopamine and histamine was evaluated. Ergometrine was found to have a good dose-dependent inotropic effect and the antagonism of cimetidine toward this action is reported. Experiments carried out in hearts in which a massive depletion of mast-cells histamine was produced are in favour of a direct action of the drug on H2-receptors."}
{"id": "PMID:463689", "title": "Combination bronchodilators: antagonism of airway smooth muscle contractions in vitro.", "content": "The component drugs of fixed-dose combination bronchodilators may interact in a synergistic manner to antagonize airway contractions. To examine this hypothesis, combinations of ephedrine (E) and theophylline (Th) or salbutamol (S) and theophylline were tested for their ability to relax contracted guinea-pig airway smooth muscle in vitro. The combination bronchodilator effect was compared to the summed effects of the component drugs given individually (i.e. a theoretical additive response, Ta). Relaxation responses to combination bronchodilators were considered less-than-additive if significantly less than Ta, additive if non-significant and greater-than-additive or synergistic if the values were significantly greater than Ta. It was found that the E-Th combinations interacted primarily in an additive fashion to relax contractions induced by histamine, acetylcholine and 5-hydroxytryptamine at concentrations that produced one-half maximal contractile response (ED50). Similarly S-Th combinations interacted in an additive manner to reverse histamine and acetylcholine contractions. In the case of both E-Th and S-Th combinations, the drugs were more effective in reducing the smooth muscle contractions when given during rather than prior to the response. It may be concluded that beta-sympathomimetics and methylxanthines when combined do not interact in a synergistic fashion to produce relaxation of contracted airway smooth muscle.", "contents": "Combination bronchodilators: antagonism of airway smooth muscle contractions in vitro. The component drugs of fixed-dose combination bronchodilators may interact in a synergistic manner to antagonize airway contractions. To examine this hypothesis, combinations of ephedrine (E) and theophylline (Th) or salbutamol (S) and theophylline were tested for their ability to relax contracted guinea-pig airway smooth muscle in vitro. The combination bronchodilator effect was compared to the summed effects of the component drugs given individually (i.e. a theoretical additive response, Ta). Relaxation responses to combination bronchodilators were considered less-than-additive if significantly less than Ta, additive if non-significant and greater-than-additive or synergistic if the values were significantly greater than Ta. It was found that the E-Th combinations interacted primarily in an additive fashion to relax contractions induced by histamine, acetylcholine and 5-hydroxytryptamine at concentrations that produced one-half maximal contractile response (ED50). Similarly S-Th combinations interacted in an additive manner to reverse histamine and acetylcholine contractions. In the case of both E-Th and S-Th combinations, the drugs were more effective in reducing the smooth muscle contractions when given during rather than prior to the response. It may be concluded that beta-sympathomimetics and methylxanthines when combined do not interact in a synergistic fashion to produce relaxation of contracted airway smooth muscle."}
{"id": "PMID:463718", "title": "Dysphagia: an evaluation and treatment program for the adult.", "content": "Dysphagia, or difficulty swallowing, can interfere with independent feeding in the physically disabled adult. The physiology of normal swallowing and neuromuscular pathology of dysphagia applicable to the occupational therapist are reviewed in this paper. Pertinent nursing care of the respiratory and gastrointestinal tracts are described. Normal and pathological reflexes involved in swallowing include gag, bite, and suck-swallow. Head, neck, and jaw stability are necessary for swallowing. Methods for evaluating the appropriate reflexes, sensation, and orofacial movement of the adult neurologically impaired patient are presented. Proper positioning is described and treatment suggestions are offered and applied to four case studies.", "contents": "Dysphagia: an evaluation and treatment program for the adult. Dysphagia, or difficulty swallowing, can interfere with independent feeding in the physically disabled adult. The physiology of normal swallowing and neuromuscular pathology of dysphagia applicable to the occupational therapist are reviewed in this paper. Pertinent nursing care of the respiratory and gastrointestinal tracts are described. Normal and pathological reflexes involved in swallowing include gag, bite, and suck-swallow. Head, neck, and jaw stability are necessary for swallowing. Methods for evaluating the appropriate reflexes, sensation, and orofacial movement of the adult neurologically impaired patient are presented. Proper positioning is described and treatment suggestions are offered and applied to four case studies."}
{"id": "PMID:463714", "title": "IgE levels of infants with complications after pertussis vaccination.", "content": "IgE levels of 31 infants who had severe complications after whooping cough vaccination, were determined by RIST. The patients had convulsions, encephalitis or anaphylactic shock symptoms. All but two had elevated IgE levels for their age. It is suggested that, in certain genetically predisposed human beings, pertussis antigen can also induce IgE synthesis, as has already been established in rodents.", "contents": "IgE levels of infants with complications after pertussis vaccination. IgE levels of 31 infants who had severe complications after whooping cough vaccination, were determined by RIST. The patients had convulsions, encephalitis or anaphylactic shock symptoms. All but two had elevated IgE levels for their age. It is suggested that, in certain genetically predisposed human beings, pertussis antigen can also induce IgE synthesis, as has already been established in rodents."}
{"id": "PMID:463715", "title": "[Bonchodynamic tests in the study of bronchial asthma].", "content": "One characteristic of chronic obstructive bronchopneumopathies, especially bronchial asthma, is the slowing down of respiratory flow. Spirographic studies are therefore valued highly among specialists in respiratory pathology. All of those working in this field of physiopathology, however, agree that broncodynamic provocation tests are not fully specific for bronchial asthma. One important problem is the difficulty in determining the quantity of product inhaled by the patient, making the test even less precise. With our method, based on the broncotest developed by Piglowski, we are able to determine the quantity of inhaled material with a fairly high degree of accuracy. It thus represents a quantitative study and is much more specific. One hundred patients with bronchial asthma, whose ages ranged from 14 to 60 years, were selected for this study. We found that 100% of them (except for one 16-year-old patient whose case has been commented on) were positive for the acetylcholine aerosol test and for beta-2adrenergics (phenotherol). These results enabled us to establish diagnostic patterns for the severity of bronchial hyperactivity in relation to the number of micrograms needed to provoke a 20% variation in the VEMS. We consider it very important to determine the threshold does of acetylcholine, since it may serve in both diagnosis and prognosis. We believe that this method may represent a new direction in pharmacodynamic examination of asthma patients.", "contents": "[Bonchodynamic tests in the study of bronchial asthma]. One characteristic of chronic obstructive bronchopneumopathies, especially bronchial asthma, is the slowing down of respiratory flow. Spirographic studies are therefore valued highly among specialists in respiratory pathology. All of those working in this field of physiopathology, however, agree that broncodynamic provocation tests are not fully specific for bronchial asthma. One important problem is the difficulty in determining the quantity of product inhaled by the patient, making the test even less precise. With our method, based on the broncotest developed by Piglowski, we are able to determine the quantity of inhaled material with a fairly high degree of accuracy. It thus represents a quantitative study and is much more specific. One hundred patients with bronchial asthma, whose ages ranged from 14 to 60 years, were selected for this study. We found that 100% of them (except for one 16-year-old patient whose case has been commented on) were positive for the acetylcholine aerosol test and for beta-2adrenergics (phenotherol). These results enabled us to establish diagnostic patterns for the severity of bronchial hyperactivity in relation to the number of micrograms needed to provoke a 20% variation in the VEMS. We consider it very important to determine the threshold does of acetylcholine, since it may serve in both diagnosis and prognosis. We believe that this method may represent a new direction in pharmacodynamic examination of asthma patients."}
{"id": "PMID:463717", "title": "[Sensitization to digoxin with demostration of reagini antibodies].", "content": "The author presents the case of a male patient, 60 years old, suffering a chronic obstructive bronchopneumopathy with cor pulmonale in a state of cardiorespiratory insuffiency. Without any personal or familial history of allergy, a few minutes after the ingestion of .5mg of Digoxin a picture occurs of urticaria, angioneurotic edema and asthmatic crisis that disappears with sympatheticomimetics. No other medication was used, previously or at the time. The existence of reaginic antibodies is made evident with positive reactions to Digoxin and C Lanatosid in a Leftwich passive transference test and cutaneous tests. No alteration was found in the number of platelets and leucocytes nor in the percentage of eosinophils within 24 hours after the anaphylactic accident. The oral provocation test with the excipient of the commercial preparation was negative; the tolerance to Methyl-Digoxin complete. The literature referring to sensitization to digitalis glycosides is reviewed and some consideration is given to its mechanism.", "contents": "[Sensitization to digoxin with demostration of reagini antibodies]. The author presents the case of a male patient, 60 years old, suffering a chronic obstructive bronchopneumopathy with cor pulmonale in a state of cardiorespiratory insuffiency. Without any personal or familial history of allergy, a few minutes after the ingestion of .5mg of Digoxin a picture occurs of urticaria, angioneurotic edema and asthmatic crisis that disappears with sympatheticomimetics. No other medication was used, previously or at the time. The existence of reaginic antibodies is made evident with positive reactions to Digoxin and C Lanatosid in a Leftwich passive transference test and cutaneous tests. No alteration was found in the number of platelets and leucocytes nor in the percentage of eosinophils within 24 hours after the anaphylactic accident. The oral provocation test with the excipient of the commercial preparation was negative; the tolerance to Methyl-Digoxin complete. The literature referring to sensitization to digitalis glycosides is reviewed and some consideration is given to its mechanism."}
{"id": "PMID:463716", "title": "The protective effect of Ketotifen in respiratory allergy.", "content": "During the last two years many works have dealt with the anti-anaphylactic properties of HC 20-511, which chemically belongs to the cycloheptathiophene group of drugs. The excellent results obtained by different authors using this drug in various allergies, decided us to study its protective use in patients affected by rhinitis and/or extrinsec bronchial asthma, especially in connection with pollinoisis. We selected a group of patients of both sexes over 12 years of age, including those who had already received immunotherapy treatment. Diagnosis was determined by means of a clear clinical history, skin tests, and exposure tests to specific antigens, using the Gronemeyer and Fuchs method. The drug was administered orally (1 mg./twice a day) for 3 months. Exposition tests were carried out before beginning treatment and at 30 and 90 days, and measurement of vital capacity, FEV1, Maximal flow and Pneumometry were carried out. A check on the clinical state of the patient, as well as on his tolerance to the drug, and on additional intake of other anti-asthmatic drugs (corticoids, Beta-stimulants) was made every 15 days. The results were highly satisfactory and a positive improvement in the clinical state of 70% of the patients after three months of treatment was noticed. When carrying out bronchial provocation tests, a minor decrease in FEV1 as well as in bronchopneumetric values were undoubtedly noticed in the patients under treatment when compared with the basal tests. Tolerance to the drug was excellent, its undesirable effects being secondary to its antihistaminic activity.", "contents": "The protective effect of Ketotifen in respiratory allergy. During the last two years many works have dealt with the anti-anaphylactic properties of HC 20-511, which chemically belongs to the cycloheptathiophene group of drugs. The excellent results obtained by different authors using this drug in various allergies, decided us to study its protective use in patients affected by rhinitis and/or extrinsec bronchial asthma, especially in connection with pollinoisis. We selected a group of patients of both sexes over 12 years of age, including those who had already received immunotherapy treatment. Diagnosis was determined by means of a clear clinical history, skin tests, and exposure tests to specific antigens, using the Gronemeyer and Fuchs method. The drug was administered orally (1 mg./twice a day) for 3 months. Exposition tests were carried out before beginning treatment and at 30 and 90 days, and measurement of vital capacity, FEV1, Maximal flow and Pneumometry were carried out. A check on the clinical state of the patient, as well as on his tolerance to the drug, and on additional intake of other anti-asthmatic drugs (corticoids, Beta-stimulants) was made every 15 days. The results were highly satisfactory and a positive improvement in the clinical state of 70% of the patients after three months of treatment was noticed. When carrying out bronchial provocation tests, a minor decrease in FEV1 as well as in bronchopneumetric values were undoubtedly noticed in the patients under treatment when compared with the basal tests. Tolerance to the drug was excellent, its undesirable effects being secondary to its antihistaminic activity."}
{"id": "PMID:463729", "title": "Malaria--a red alert.", "content": "Imported malaria has been increasing in the United States. Locally acquired cases are reported in drug addicts. Extended incubation periods can be misleading. Pyrexia is the most common manifestation, but it may not follow a regular pattern. A high index of suspicion and examination of peripheral blood smears establish the diagnosis. Chloroquine and primaquine are the drugs of choice for uncomplicated malaria; quinine sulfate is preferred in the treatment of chloroquine-resistant malaria. Malarial prophylaxis should include travelers' education and chemoprophylactic agents such as chloroquine, chloroguanide, pyrimethamine and amodiaquine.", "contents": "Malaria--a red alert. Imported malaria has been increasing in the United States. Locally acquired cases are reported in drug addicts. Extended incubation periods can be misleading. Pyrexia is the most common manifestation, but it may not follow a regular pattern. A high index of suspicion and examination of peripheral blood smears establish the diagnosis. Chloroquine and primaquine are the drugs of choice for uncomplicated malaria; quinine sulfate is preferred in the treatment of chloroquine-resistant malaria. Malarial prophylaxis should include travelers' education and chemoprophylactic agents such as chloroquine, chloroguanide, pyrimethamine and amodiaquine."}
{"id": "PMID:463731", "title": "Physical examination of scuba divers.", "content": "With the increasing popularity of courses in scuba diving, prospective diving students coming to physicians for medical examinations are presenting a wide range of ages and physical makeup. A working knowledge of medical fitness for diving and of diving medical emergencies will assist the nondiving physician in establishing a basic medical history and examination for the student scuba diver.", "contents": "Physical examination of scuba divers. With the increasing popularity of courses in scuba diving, prospective diving students coming to physicians for medical examinations are presenting a wide range of ages and physical makeup. A working knowledge of medical fitness for diving and of diving medical emergencies will assist the nondiving physician in establishing a basic medical history and examination for the student scuba diver."}
{"id": "PMID:463746", "title": "A modified impinger for personal sampling.", "content": "The performance of a modified impinger is described. Laboratory and limited field studies found that the modification made the device relatively spill-proof. In addition, the collection efficiency of the spill-proof impinger was compared to the standard midget impinger. For particles larger than 0.8 micrometer equivalent aerodynamic diameter, the impaction efficiencies of the two devices were found to be identical. However, the capture efficiencies of the two devices were not always found to be equivalent. In three tests, the modified impinger collected proportionately more material than the midget impinger. In three other tests, the capture efficiencies of the two devices were found to be equivalent.", "contents": "A modified impinger for personal sampling. The performance of a modified impinger is described. Laboratory and limited field studies found that the modification made the device relatively spill-proof. In addition, the collection efficiency of the spill-proof impinger was compared to the standard midget impinger. For particles larger than 0.8 micrometer equivalent aerodynamic diameter, the impaction efficiencies of the two devices were found to be identical. However, the capture efficiencies of the two devices were not always found to be equivalent. In three tests, the modified impinger collected proportionately more material than the midget impinger. In three other tests, the capture efficiencies of the two devices were found to be equivalent."}
{"id": "PMID:463747", "title": "Personnel monitoring for tetraalkyl lead in the workplace.", "content": "Personnel monitoring for alkyl lead and inorganic lead in air was carried out for six weeks on five workers in an alkyl lead manufacturing plant. Lead in air values were compared to biological values for lead blood, lead in urine, and delta aminoleuvlinic acid in urine. While variations in each of these measurements occurred, there was no correlation between any of these three measurements and lead in air. Measurements of atmospheric exposure to alkyl lead by personnel monitoring does not provide an accurate assessment of the degree of hazard to workers. When such measurements are performed frequently, they can provide information useful in controlling respiratory exposure. Biological monitoring of lead in urine as practiced by the alkyl lead industry remains the best protection against overexposure to organic lead.", "contents": "Personnel monitoring for tetraalkyl lead in the workplace. Personnel monitoring for alkyl lead and inorganic lead in air was carried out for six weeks on five workers in an alkyl lead manufacturing plant. Lead in air values were compared to biological values for lead blood, lead in urine, and delta aminoleuvlinic acid in urine. While variations in each of these measurements occurred, there was no correlation between any of these three measurements and lead in air. Measurements of atmospheric exposure to alkyl lead by personnel monitoring does not provide an accurate assessment of the degree of hazard to workers. When such measurements are performed frequently, they can provide information useful in controlling respiratory exposure. Biological monitoring of lead in urine as practiced by the alkyl lead industry remains the best protection against overexposure to organic lead."}
{"id": "PMID:463748", "title": "Determination of airborne organic vapor mixtures using charcoal tubes.", "content": "The charcoal tube method for organic vapor analysis has been extensively utilized by this laboratory to monitor factory environments throughout a multisite manufacturing complex. Atmospheres sampled generally contain several chemicals that contribute to total exposure, and it is necessary to modify procedures developed for individual compounds. Examples of such alterations, to effect acceptable collection/recovery of organic analytes, are cited. Topics discussed include selection of desorption solvent, influences of temperature and polar compounds on desorption efficiency, and overall efficiency as a superior measure of method validity. A simple qualitative analysis scheme described in the literature is used to suport identification of chromatographic peaks. A critique of this technique is presented along with a practical factory analysis application.", "contents": "Determination of airborne organic vapor mixtures using charcoal tubes. The charcoal tube method for organic vapor analysis has been extensively utilized by this laboratory to monitor factory environments throughout a multisite manufacturing complex. Atmospheres sampled generally contain several chemicals that contribute to total exposure, and it is necessary to modify procedures developed for individual compounds. Examples of such alterations, to effect acceptable collection/recovery of organic analytes, are cited. Topics discussed include selection of desorption solvent, influences of temperature and polar compounds on desorption efficiency, and overall efficiency as a superior measure of method validity. A simple qualitative analysis scheme described in the literature is used to suport identification of chromatographic peaks. A critique of this technique is presented along with a practical factory analysis application."}
{"id": "PMID:463749", "title": "Field evaluation of selected monitoring methods for phosgene in air.", "content": "Four selected methods have been evaluated and field tested as candidates for a specific, continuous procedure for monitoring phosgene in air at or below concentrations of 0.05 ppm. The methods evaluated were automated colorimetry, gas chromatography, infrared spectrophotometry and a recently developed paper tape monitor. A standard manual colorimetric procedure was used as a reference method. The paper tape, infrared and automated gas chromatographic techniques most closely fulfilled the criteria set forth for an \"ideal\", selective, continuous procedure for phosgene.", "contents": "Field evaluation of selected monitoring methods for phosgene in air. Four selected methods have been evaluated and field tested as candidates for a specific, continuous procedure for monitoring phosgene in air at or below concentrations of 0.05 ppm. The methods evaluated were automated colorimetry, gas chromatography, infrared spectrophotometry and a recently developed paper tape monitor. A standard manual colorimetric procedure was used as a reference method. The paper tape, infrared and automated gas chromatographic techniques most closely fulfilled the criteria set forth for an \"ideal\", selective, continuous procedure for phosgene."}
{"id": "PMID:463750", "title": "The nuclear worker and ionizing radiation.", "content": "Research on medical X-ray exposure sheds light on the health effects expected for workers exposed to ionizing radiation. Factual information confirming this relationship and also demonstrating the need for reviewing permissible exposure levels for workers is given. Suggestions for more sophisticated health monitoring of workers exposed to hazardous technologies are made, and the question of occupational hazards which threaten the worker's family is raised.", "contents": "The nuclear worker and ionizing radiation. Research on medical X-ray exposure sheds light on the health effects expected for workers exposed to ionizing radiation. Factual information confirming this relationship and also demonstrating the need for reviewing permissible exposure levels for workers is given. Suggestions for more sophisticated health monitoring of workers exposed to hazardous technologies are made, and the question of occupational hazards which threaten the worker's family is raised."}
{"id": "PMID:463751", "title": "Drywall construction and asbestos exposure.", "content": "The rapid development of the drywall construction trade in the United States is described. It is estimated that some 75,000 U.S. construction workers are currently employed in this trade. The use of a variety of spackle and taping compounds is shown to be associated with significant asbestos exposure; air samples taken in the breathing zone by drywall tapers during sanding of taping compounds show fiber concentrations exceeding, by several times, the maximum level permitted by United States Government regulations. These findings are given together with the result of a clinical field survey of drywall construction workers demonstrating that asbestos disease may be an important health hazard in this trade.", "contents": "Drywall construction and asbestos exposure. The rapid development of the drywall construction trade in the United States is described. It is estimated that some 75,000 U.S. construction workers are currently employed in this trade. The use of a variety of spackle and taping compounds is shown to be associated with significant asbestos exposure; air samples taken in the breathing zone by drywall tapers during sanding of taping compounds show fiber concentrations exceeding, by several times, the maximum level permitted by United States Government regulations. These findings are given together with the result of a clinical field survey of drywall construction workers demonstrating that asbestos disease may be an important health hazard in this trade."}
{"id": "PMID:463752", "title": "Development of methodologies to assess the relative hazards from thermal decomposition products of polymeric materials.", "content": "The physiological stress imposed upon mice due to the irritating properties of thermal decomposition products of polymeric materials was evaluated. Acute lethality and histopathological evaluation were included in the study. The rankings of the polymeric materials studied from most to least hazardous was concluded to be polytetrafluoroethylene greater than polyvinyl chloride greater than Douglas Fir and flexible polyurethane foam greater than fiber glass reinforced polyester greater than copper coated wire with mineral insulation.", "contents": "Development of methodologies to assess the relative hazards from thermal decomposition products of polymeric materials. The physiological stress imposed upon mice due to the irritating properties of thermal decomposition products of polymeric materials was evaluated. Acute lethality and histopathological evaluation were included in the study. The rankings of the polymeric materials studied from most to least hazardous was concluded to be polytetrafluoroethylene greater than polyvinyl chloride greater than Douglas Fir and flexible polyurethane foam greater than fiber glass reinforced polyester greater than copper coated wire with mineral insulation."}
{"id": "PMID:463753", "title": "A six-year follow up study for evaluation of the 85 dBA safe criterion for noise exposure.", "content": "This study included 114 workers who have been exposed to noise levels of 78 to 91.4 dBA and followed over a six year period. Workers exposed to nose levels up to 85 dBA eight hours daily for six days per week and for twelve years showed a slightly higher (1%) hearing impairment than the corresponding control subjects. Impairment of hearing among workers exposed to higher noise levels was 9.6%.", "contents": "A six-year follow up study for evaluation of the 85 dBA safe criterion for noise exposure. This study included 114 workers who have been exposed to noise levels of 78 to 91.4 dBA and followed over a six year period. Workers exposed to nose levels up to 85 dBA eight hours daily for six days per week and for twelve years showed a slightly higher (1%) hearing impairment than the corresponding control subjects. Impairment of hearing among workers exposed to higher noise levels was 9.6%."}
{"id": "PMID:463754", "title": "Influence of sonic noise on human stereoscopic depth perception.", "content": "Scientific establishment of the no-effect response to finite levels of exposure to a physical or chemical agent is indeed a rigorous exercise and is frequently controversial. In earlier research by Slutsky under direction of the senior author, a statistically significant increase in stereoscopic depth perception error was noted among 24 test subjects exposed to high intensity noise. Additional extensive research reported in this paper indicates that error in stereoscopic depth perception is not significantly altered by exposure to continuous white noise of short duration at levels ranging from 70 to 115 dBA. Furthernore, exposure of humans for periods of a few minutes to white noise in octave bands centered on 250 Hz, 1000 Hz, 4 kHz and 16 kHz at 115 dB does not affect their depth perception measured by the Howard-Dolman test. A comprehensive analysis of depth perception errors measured under noise exposure conditions (n = 4040) in comparison with those obtained under control conditions (n = 1430) produced a mean change in error of -0.38 mm, a statistically insignificant difference (p = 0.17). Even if such an error were attributable to high level noise, it should be noted that minus sign designates an improvement of depth perception in noise and that it is difficult to imagine visual tasks in which change in error of +/-0.38 mm at a distance of 6.0 meters is meaningful.", "contents": "Influence of sonic noise on human stereoscopic depth perception. Scientific establishment of the no-effect response to finite levels of exposure to a physical or chemical agent is indeed a rigorous exercise and is frequently controversial. In earlier research by Slutsky under direction of the senior author, a statistically significant increase in stereoscopic depth perception error was noted among 24 test subjects exposed to high intensity noise. Additional extensive research reported in this paper indicates that error in stereoscopic depth perception is not significantly altered by exposure to continuous white noise of short duration at levels ranging from 70 to 115 dBA. Furthernore, exposure of humans for periods of a few minutes to white noise in octave bands centered on 250 Hz, 1000 Hz, 4 kHz and 16 kHz at 115 dB does not affect their depth perception measured by the Howard-Dolman test. A comprehensive analysis of depth perception errors measured under noise exposure conditions (n = 4040) in comparison with those obtained under control conditions (n = 1430) produced a mean change in error of -0.38 mm, a statistically insignificant difference (p = 0.17). Even if such an error were attributable to high level noise, it should be noted that minus sign designates an improvement of depth perception in noise and that it is difficult to imagine visual tasks in which change in error of +/-0.38 mm at a distance of 6.0 meters is meaningful."}
{"id": "PMID:463756", "title": "Field comparison of two methods for the determination of NO2 concentration in air.", "content": "A field test of two sampling methods for the determination of NO2 concentration in air is reported. All samples were collected in an underground salt mine in which diesel equipment is operated. Although each method has it own advantage, both appear to be suitable for most industrial hygiene applications.", "contents": "Field comparison of two methods for the determination of NO2 concentration in air. A field test of two sampling methods for the determination of NO2 concentration in air is reported. All samples were collected in an underground salt mine in which diesel equipment is operated. Although each method has it own advantage, both appear to be suitable for most industrial hygiene applications."}
{"id": "PMID:463757", "title": "The collection of arsenic oxide vapor in water-filled impingers.", "content": "The efficiency of water as a collection medium for arsenic oxide vapor was investigated. The vapor was generated by passing air over arsenolite (As2O3, s) at various flow rates and temperatures, passed through a particulate filter and then was collected in a series of chilled Greenburg-Smith impingers. It was found that the first impinger always collected at least 90% of the vapor while the remainder, within the limit of detection, was collected in the second impinger. No differences were found between water and 2% sodium hydroxide when used as a collection medium. Up to 15% of the collected arsenic may be absorbed on the walls of the impinger train; this amount will not dissolve in water and will be lost unless the train is thoroughly washed with hydroxide.", "contents": "The collection of arsenic oxide vapor in water-filled impingers. The efficiency of water as a collection medium for arsenic oxide vapor was investigated. The vapor was generated by passing air over arsenolite (As2O3, s) at various flow rates and temperatures, passed through a particulate filter and then was collected in a series of chilled Greenburg-Smith impingers. It was found that the first impinger always collected at least 90% of the vapor while the remainder, within the limit of detection, was collected in the second impinger. No differences were found between water and 2% sodium hydroxide when used as a collection medium. Up to 15% of the collected arsenic may be absorbed on the walls of the impinger train; this amount will not dissolve in water and will be lost unless the train is thoroughly washed with hydroxide."}
{"id": "PMID:463758", "title": "Septicemia in patients with an endocardial pacemaker.", "content": "The records of 1,235 consecutive patients treated with long-term pacing by the endocardial route between 1964 and 1977 were analyzed to determine the incidence, mechanism, course and treatment of septicemia. Septicemia developed in 12 patients (1 percent), and Staphylococcus aureus was isolated from the blood culture in 10. All patients were treated with the usual prolonged course of bactericidal drugs. Treatment was successful in only two of the seven patients whose endocardial pacing system was left in place; in three of the seven the septicemia recurred, necessitating removal of the endocardial system, and two of these patients died. In the remaining four patients the endocardial wire was promptly withdrawn, with use of a thoracotomy when necessary, and an epicardial system inserted; all of these patients survived. This is the treatment of choice.", "contents": "Septicemia in patients with an endocardial pacemaker. The records of 1,235 consecutive patients treated with long-term pacing by the endocardial route between 1964 and 1977 were analyzed to determine the incidence, mechanism, course and treatment of septicemia. Septicemia developed in 12 patients (1 percent), and Staphylococcus aureus was isolated from the blood culture in 10. All patients were treated with the usual prolonged course of bactericidal drugs. Treatment was successful in only two of the seven patients whose endocardial pacing system was left in place; in three of the seven the septicemia recurred, necessitating removal of the endocardial system, and two of these patients died. In the remaining four patients the endocardial wire was promptly withdrawn, with use of a thoracotomy when necessary, and an epicardial system inserted; all of these patients survived. This is the treatment of choice."}
{"id": "PMID:463759", "title": "Intracardiac electrocardiography during permanent pacemaker implantation: predictors of cardiac perforation.", "content": "Intracardiac electrograms from 50 successive patients undergoing permanent pacemaker implantation have been analyzed. There were 29 male and 21 female patients aged 14 to 93 years (mean age 68.4 years). The electrograms were obtained using methods that simulated the wave form that would be detected by unipolar cardiac pacemakers. Three types of electrographic patterns were identified: qR pattern with a q/R ratio of less than 1 (type I): QR pattern with a Q/R ratio between 1 and 4.4 (type II); and Qr pattern with a Q/r ratio between 12 and 15 (type III). A type I pattern was seen in 29 patients (58 percent), type II in 18 (36 percent) and type III in 3 patients (6 percent). The duration of the follow-up period ranged from 3 weeks to 20 months (mean 9.7 months); three patients were lost to follow-up study. There were four deaths apparently unrelated to the pacemaker. Recognizable problems (either pacing or sensing failure) occurred in one patient (6 percent) with a type II pattern, in two patients (66.7 percent) with a type III pattern and in no patient with a type I pattern. On the basis of these data it is suggested that at the time of pacemaker implantation, intracardiac electrograms with a type I pattern indicating good pacing thresholds and sensing should be sought. If type II wave forms occur with good pacing thresholds and sensing then the electrode could probably be left in position. The incidence of a type III pattern is rare; when it does occur it is greatly suggestive of myocardial perforation. When this pattern is seen, the pacemaker catheter must be repositioned.", "contents": "Intracardiac electrocardiography during permanent pacemaker implantation: predictors of cardiac perforation. Intracardiac electrograms from 50 successive patients undergoing permanent pacemaker implantation have been analyzed. There were 29 male and 21 female patients aged 14 to 93 years (mean age 68.4 years). The electrograms were obtained using methods that simulated the wave form that would be detected by unipolar cardiac pacemakers. Three types of electrographic patterns were identified: qR pattern with a q/R ratio of less than 1 (type I): QR pattern with a Q/R ratio between 1 and 4.4 (type II); and Qr pattern with a Q/r ratio between 12 and 15 (type III). A type I pattern was seen in 29 patients (58 percent), type II in 18 (36 percent) and type III in 3 patients (6 percent). The duration of the follow-up period ranged from 3 weeks to 20 months (mean 9.7 months); three patients were lost to follow-up study. There were four deaths apparently unrelated to the pacemaker. Recognizable problems (either pacing or sensing failure) occurred in one patient (6 percent) with a type II pattern, in two patients (66.7 percent) with a type III pattern and in no patient with a type I pattern. On the basis of these data it is suggested that at the time of pacemaker implantation, intracardiac electrograms with a type I pattern indicating good pacing thresholds and sensing should be sought. If type II wave forms occur with good pacing thresholds and sensing then the electrode could probably be left in position. The incidence of a type III pattern is rare; when it does occur it is greatly suggestive of myocardial perforation. When this pattern is seen, the pacemaker catheter must be repositioned."}
{"id": "PMID:463760", "title": "Two dimensional echocardiographic recognition of the descending thoracic aorta.", "content": "Two dimensional echocardiography was used to identify the descending thoracic aorta in 106 subjects. In 12 of these subjects, contrast injection techniques were used to identify this structure as it descended posteriorly adjacent to the atrioventricular groove. The course of the descending thoracic aorta was mapped using both the long axis and transverse axis views. The normal descending thoracic aorta (26 subjects) measured 10 +/- 1.4 mm/m2 during diastole. Unusual M mode echocardiographic patterns of the descending aorta may be confused with other disease states; they are clarified with the two dimensional study. The size and appearance of the descending aorta in different types of cardiovascular disease, including aortic aneurysm, in 80 patients are described. It is anticipated that two dimensional echocardiography will prove to be a useful method of studying patients with aortic disease.", "contents": "Two dimensional echocardiographic recognition of the descending thoracic aorta. Two dimensional echocardiography was used to identify the descending thoracic aorta in 106 subjects. In 12 of these subjects, contrast injection techniques were used to identify this structure as it descended posteriorly adjacent to the atrioventricular groove. The course of the descending thoracic aorta was mapped using both the long axis and transverse axis views. The normal descending thoracic aorta (26 subjects) measured 10 +/- 1.4 mm/m2 during diastole. Unusual M mode echocardiographic patterns of the descending aorta may be confused with other disease states; they are clarified with the two dimensional study. The size and appearance of the descending aorta in different types of cardiovascular disease, including aortic aneurysm, in 80 patients are described. It is anticipated that two dimensional echocardiography will prove to be a useful method of studying patients with aortic disease."}
{"id": "PMID:463762", "title": "Q-aTc interval as a clinical indicator of hypercalcemia.", "content": "The charts were reviewed of more than 200 patients discharged from the hospital between 1972 and 1977 with a diagnosis implying abnormal calcium metabolism. When all other variables known to affect the Q-T interval were eliminated, serum calcium concentrations on 65 occasions in 39 patients were found to correlate significantly (P less than 0.001 or better) with the following electrocardiographic intervals: Q-aTc (beginning of QRS complex to apex of T wave), Q-oTc (beginning of QRS complex to onset of T wave) and Q-Tc (beginning of QRS complex to end of T wave). Of these, the Q-aTc interval was the more easily and precisely measured at elevated calcium levels and exhibited the strongest correlation (P less than 0.0001) over the range of calcium levels measured. The relation was linear and could be used to estimate serum calcium levels from measured Q-aTc intervals. For a Q-aTc interval of 0.29 second or less (range 0.23 to 0.29) the serum calcium was estimated to +/- 1.9 mg/dl with 95 percent confidence. For a Q-aTc interval of 0.27 second or less, the correspondence with hypercalcemia in the study series was 90 percent or better. When all other factors known to affect the Q-T interval are ruled out, the shortening of the Q-aTc interval appears to be a useful clinical indicator of hypercalcemia.", "contents": "Q-aTc interval as a clinical indicator of hypercalcemia. The charts were reviewed of more than 200 patients discharged from the hospital between 1972 and 1977 with a diagnosis implying abnormal calcium metabolism. When all other variables known to affect the Q-T interval were eliminated, serum calcium concentrations on 65 occasions in 39 patients were found to correlate significantly (P less than 0.001 or better) with the following electrocardiographic intervals: Q-aTc (beginning of QRS complex to apex of T wave), Q-oTc (beginning of QRS complex to onset of T wave) and Q-Tc (beginning of QRS complex to end of T wave). Of these, the Q-aTc interval was the more easily and precisely measured at elevated calcium levels and exhibited the strongest correlation (P less than 0.0001) over the range of calcium levels measured. The relation was linear and could be used to estimate serum calcium levels from measured Q-aTc intervals. For a Q-aTc interval of 0.29 second or less (range 0.23 to 0.29) the serum calcium was estimated to +/- 1.9 mg/dl with 95 percent confidence. For a Q-aTc interval of 0.27 second or less, the correspondence with hypercalcemia in the study series was 90 percent or better. When all other factors known to affect the Q-T interval are ruled out, the shortening of the Q-aTc interval appears to be a useful clinical indicator of hypercalcemia."}
{"id": "PMID:463765", "title": "Right and left ventricular function after the Mustard procedure in transposition of the great arteries.", "content": "Right and left ventricular function was assessed at cardiac catheterization in 33 asymptomatic patients 0.5 to 11 years (mean 4.6) after the Mustard operation for complete transposition of the great arteries. Ages at operation had ranged from 0.5 to 16 years (mean 4.2 years). Right ventricular function was assessed using videodensitometric determination of ejection fraction and ventricular volume data. Ventricular volumes were obtained by computerized video analysis utilizing Simpson's rule. The right ventricular ejection fraction was 37 +/- 11 percent (standard deviation), as assessed with videodensitometry and 42 +/- 10 percent as assessed with ventricular volume--both values less than normal (P less than 0.001). Right ventricular end-diastolic volume was significantly greater than normal (P less than 0.001) and averaged 202 +/- 70 percent, but left ventricular end-diastolic volume averaged only 125 +/- 53 percent. These observations after the Mustard operation indicate that right ventricular function is seriously decreased with relatively preserved left ventricular function. They support efforts for surgical correction utilizing the left ventricle as the systemic ventricle.", "contents": "Right and left ventricular function after the Mustard procedure in transposition of the great arteries. Right and left ventricular function was assessed at cardiac catheterization in 33 asymptomatic patients 0.5 to 11 years (mean 4.6) after the Mustard operation for complete transposition of the great arteries. Ages at operation had ranged from 0.5 to 16 years (mean 4.2 years). Right ventricular function was assessed using videodensitometric determination of ejection fraction and ventricular volume data. Ventricular volumes were obtained by computerized video analysis utilizing Simpson's rule. The right ventricular ejection fraction was 37 +/- 11 percent (standard deviation), as assessed with videodensitometry and 42 +/- 10 percent as assessed with ventricular volume--both values less than normal (P less than 0.001). Right ventricular end-diastolic volume was significantly greater than normal (P less than 0.001) and averaged 202 +/- 70 percent, but left ventricular end-diastolic volume averaged only 125 +/- 53 percent. These observations after the Mustard operation indicate that right ventricular function is seriously decreased with relatively preserved left ventricular function. They support efforts for surgical correction utilizing the left ventricle as the systemic ventricle."}
{"id": "PMID:463766", "title": "Quantitative analysis of postoperative changes in the pulmonary vasculature of patients with complete transposition of the great arteries and pulmonary hypertension.", "content": "Postoperative changes in the medial thickness of the small pulmonary arteries and the degree of pulmonary vascular disease were estimated histometrically and histopathologically in three cases of late death after total correction of complete transposition of the great arteries with large ventricular septal defect and pulmonary hypertension. In the postoperative course of two of the three cases extreme medial hypertrophy of the small pulmonary arteries as well as severe pulmonary vascular disease were found. In the third case, the thickening of the media was mild and pulmonary vascular disease had not progressed owing to a residual ventricular septal defect. Examination of three additional cases of late death and 15 autopsy cases of complete transposition of the great arteries revealed that hypertrophy of pulmonary arterial media after radical surgery for complete transposition of the great arteries is a common phenomenon. In cases of complete transposition of the great arteries with severe pulmonary hypertension, the deveopment of marked hypertrophy of the media accompanied by pulmonary vascular disease after total correction is usually seen and seems to be the most likely cause of death in the postoperative period.", "contents": "Quantitative analysis of postoperative changes in the pulmonary vasculature of patients with complete transposition of the great arteries and pulmonary hypertension. Postoperative changes in the medial thickness of the small pulmonary arteries and the degree of pulmonary vascular disease were estimated histometrically and histopathologically in three cases of late death after total correction of complete transposition of the great arteries with large ventricular septal defect and pulmonary hypertension. In the postoperative course of two of the three cases extreme medial hypertrophy of the small pulmonary arteries as well as severe pulmonary vascular disease were found. In the third case, the thickening of the media was mild and pulmonary vascular disease had not progressed owing to a residual ventricular septal defect. Examination of three additional cases of late death and 15 autopsy cases of complete transposition of the great arteries revealed that hypertrophy of pulmonary arterial media after radical surgery for complete transposition of the great arteries is a common phenomenon. In cases of complete transposition of the great arteries with severe pulmonary hypertension, the deveopment of marked hypertrophy of the media accompanied by pulmonary vascular disease after total correction is usually seen and seems to be the most likely cause of death in the postoperative period."}
{"id": "PMID:463769", "title": "Differences in hemodynamic effects of nitroprusside and prazosin in severe chronic congestive heart failure: evidence for a direct negative chronotropic effect of prazosin.", "content": "To compare the hemodynamic effects of prazosin and nitroprusside in patients with severe congestive heart failure, nine patients with heart failure refractory to conventional therapy received oral prazosin and intravenous nitroprusside administered so as to produce a similar decrease in left ventricular filling pressure in each patient. By this comparison, both drugs produced similar decreases in mean right atrial pressure, mean pulmonary arterial pressure and systemic and pulmonary vascular resistance. However, with nitroprusside, cardiac index increased more (+0.97 versus +0.73 liters/min per m2, P less than 0.01) and mean arterial pressure decreased less (-13.7 versus -18.3 mm Hg, P less than 0.05) than with prazosin. Both drugs produced similar changes in stroke volume index (+11.7 cc/beat per m2 with nitroprusside and +12.5 with prazosin) and stroke work index (+8.1 g-m/m2 with nitroprusside and +6.6 with prazosin). Therefore, the differences in the hemodynamic responses observed with the two agents were due to the significantly greater decrease in heart rate with prazosin (-8 beats/min) than with nitroprusside (-2 beats/min, P less than 0.05). These clinical data support experimental evidence suggesting that there is a significant negative chronotropic action of prazosin independent of its peripheral vascular effects.", "contents": "Differences in hemodynamic effects of nitroprusside and prazosin in severe chronic congestive heart failure: evidence for a direct negative chronotropic effect of prazosin. To compare the hemodynamic effects of prazosin and nitroprusside in patients with severe congestive heart failure, nine patients with heart failure refractory to conventional therapy received oral prazosin and intravenous nitroprusside administered so as to produce a similar decrease in left ventricular filling pressure in each patient. By this comparison, both drugs produced similar decreases in mean right atrial pressure, mean pulmonary arterial pressure and systemic and pulmonary vascular resistance. However, with nitroprusside, cardiac index increased more (+0.97 versus +0.73 liters/min per m2, P less than 0.01) and mean arterial pressure decreased less (-13.7 versus -18.3 mm Hg, P less than 0.05) than with prazosin. Both drugs produced similar changes in stroke volume index (+11.7 cc/beat per m2 with nitroprusside and +12.5 with prazosin) and stroke work index (+8.1 g-m/m2 with nitroprusside and +6.6 with prazosin). Therefore, the differences in the hemodynamic responses observed with the two agents were due to the significantly greater decrease in heart rate with prazosin (-8 beats/min) than with nitroprusside (-2 beats/min, P less than 0.05). These clinical data support experimental evidence suggesting that there is a significant negative chronotropic action of prazosin independent of its peripheral vascular effects."}
{"id": "PMID:463771", "title": "Effects of antihypertensive therapy on cardiovascular response to exercise.", "content": "The effects of alpha methyldopa and hydrochlorothiazide therapy on exercise induced changes in arterial pressure, heart rate, rate-pressure product and total duration of exercise were evaluated with graded treadmill exercise. Although both agents were equally effective in reducing resting arterial pressure, alpha methyldopa appeared to provide slower build-up, a lower ceiling and more rapid recovery of arterial pressure in response to exercise stress than did hydrochlorothizide. Alpha methyldopa significantly reduced the degree of rise of the rate-pressure product (an index of myocardial oxygen consumption) during the exercise and recovery phases, whereas hydrochlorothiazide failed to do so. The total duration of exercise (an index of work capacity) was unchanged with either hydrochlorothiazide or alpha methyldopa. Therapy with alpha methyldopa presumably reduced myocardial oxygen demand without reducing work capacity.", "contents": "Effects of antihypertensive therapy on cardiovascular response to exercise. The effects of alpha methyldopa and hydrochlorothiazide therapy on exercise induced changes in arterial pressure, heart rate, rate-pressure product and total duration of exercise were evaluated with graded treadmill exercise. Although both agents were equally effective in reducing resting arterial pressure, alpha methyldopa appeared to provide slower build-up, a lower ceiling and more rapid recovery of arterial pressure in response to exercise stress than did hydrochlorothizide. Alpha methyldopa significantly reduced the degree of rise of the rate-pressure product (an index of myocardial oxygen consumption) during the exercise and recovery phases, whereas hydrochlorothiazide failed to do so. The total duration of exercise (an index of work capacity) was unchanged with either hydrochlorothiazide or alpha methyldopa. Therapy with alpha methyldopa presumably reduced myocardial oxygen demand without reducing work capacity."}
{"id": "PMID:463772", "title": "Management of intractable ventricular tachyarrhythmias after myocardial infarction.", "content": "Twenty-five patients with recent or old myocardial infarction were studied because they had life-threatening ventricular arrhythmias that required repeated cardioversions and were intractable to medical management. All patients had had a large anterior infarction a mean of 4.6 weeks before the emergence of the arrhythmias and all had severe left ventricular dysfunction. Cardiac catheterization or autopsy revealed a left ventricular aneurysm in 18 of 18 patients and obstruction of the left anterior descending coronary artery in 20 of 20 patients. Of 16 patients treated surgically with aneurysm resection or coronary bypass grafting, or both, 10 (62 percent) were alive after 3 to 39 (mean 26) months of follow-up. The perioperative mortality rate was 31 percent and only one patient died during the postoperative follow-up period 4 months after discharge from the hospital. By contrast, all nine medically treated patients died either in the hospital (four patients) or suddenly within 2 months of discharge (five patients). Ventricular fibrillation was documented as the cause of death in five of these patients. Surgical intervention was found to improve significantly the survival of these patients (P less than 0.02). The perioperative mortality rate was lower when at least 4 weeks had elapsed from acute infarction to surgery (10 versus 67 percent) and when the procedure included coronary bypass grafting (13 versus 50 percent), although these differences were not statistically significant (P greater than 0.05).", "contents": "Management of intractable ventricular tachyarrhythmias after myocardial infarction. Twenty-five patients with recent or old myocardial infarction were studied because they had life-threatening ventricular arrhythmias that required repeated cardioversions and were intractable to medical management. All patients had had a large anterior infarction a mean of 4.6 weeks before the emergence of the arrhythmias and all had severe left ventricular dysfunction. Cardiac catheterization or autopsy revealed a left ventricular aneurysm in 18 of 18 patients and obstruction of the left anterior descending coronary artery in 20 of 20 patients. Of 16 patients treated surgically with aneurysm resection or coronary bypass grafting, or both, 10 (62 percent) were alive after 3 to 39 (mean 26) months of follow-up. The perioperative mortality rate was 31 percent and only one patient died during the postoperative follow-up period 4 months after discharge from the hospital. By contrast, all nine medically treated patients died either in the hospital (four patients) or suddenly within 2 months of discharge (five patients). Ventricular fibrillation was documented as the cause of death in five of these patients. Surgical intervention was found to improve significantly the survival of these patients (P less than 0.02). The perioperative mortality rate was lower when at least 4 weeks had elapsed from acute infarction to surgery (10 versus 67 percent) and when the procedure included coronary bypass grafting (13 versus 50 percent), although these differences were not statistically significant (P greater than 0.05)."}
{"id": "PMID:463773", "title": "Polymorphous ventricular tachycardia: clinical features and treatment.", "content": "Thirty-four cases of ventricular tachyarrhythmia characterized by polymorphy of the QRS complexes with changing R-R intervals and a heart rate of 150 to 300 beats/min, termed polymorphous ventricular tachycardia, are described. The factors involved in the appearance of this arrhythmia were the administration of antiarrhythmic drugs (quinidine 22 patients, procainamide 5 patients, ajmaline 1 patient), antianginal drugs (prenylamine [Synadrin] 4 patients) and antidepressant drugs (thioridazine 1 patient). Twenty-one patients were treated for premature ventricular complexes, three for chronic recurrent ventricular tachycardia, six for atrial flutter and fibrillation, three for anginal pain and one patient for mental depression. All patients except one had a drug-induced prolonged corrected Q-T interval before the appearance of polymorphous ventricular tachycardia. Most of the patients with this arrhythmia were considered to have severe myocardial disease. Lidocaine and electric cardioversion were administered to all patients, but were effective only in seven patients whose tachycardia occurred in short, single episodes. The most effective treatment (17 patients) was temporary ventricular pacing at rates ranging from 100 to 140 beats/min. Intravenous isoproterenol proved to be successful in another 10 cases. It is concluded that patients with severe myocardial involvement receiving antiarrhythmic drugs for premature ventricular complexes, especially the multiform variety, are at high risk for the development of polymorphous ventricular tachycardia.", "contents": "Polymorphous ventricular tachycardia: clinical features and treatment. Thirty-four cases of ventricular tachyarrhythmia characterized by polymorphy of the QRS complexes with changing R-R intervals and a heart rate of 150 to 300 beats/min, termed polymorphous ventricular tachycardia, are described. The factors involved in the appearance of this arrhythmia were the administration of antiarrhythmic drugs (quinidine 22 patients, procainamide 5 patients, ajmaline 1 patient), antianginal drugs (prenylamine [Synadrin] 4 patients) and antidepressant drugs (thioridazine 1 patient). Twenty-one patients were treated for premature ventricular complexes, three for chronic recurrent ventricular tachycardia, six for atrial flutter and fibrillation, three for anginal pain and one patient for mental depression. All patients except one had a drug-induced prolonged corrected Q-T interval before the appearance of polymorphous ventricular tachycardia. Most of the patients with this arrhythmia were considered to have severe myocardial disease. Lidocaine and electric cardioversion were administered to all patients, but were effective only in seven patients whose tachycardia occurred in short, single episodes. The most effective treatment (17 patients) was temporary ventricular pacing at rates ranging from 100 to 140 beats/min. Intravenous isoproterenol proved to be successful in another 10 cases. It is concluded that patients with severe myocardial involvement receiving antiarrhythmic drugs for premature ventricular complexes, especially the multiform variety, are at high risk for the development of polymorphous ventricular tachycardia."}
{"id": "PMID:463774", "title": "Noninvasive diagnosis of left ventricular pseudoaneurysm: role of two dimensional echocardiography and radionuclide gated pool imaging.", "content": "This report describes a case of left ventricular pseudoaneurysm diagnosed with two dimensional real time echocardiography. The two dimensional echocardiogram identified not only the pseudoaneurysmal sac but also the site of left ventricular rupture. When supplemented with radioisotope gated cardiac blood pool scanning, the noninvasive studies demonstrated combined true and false left ventricular aneurysms. Left ventricular pseudoaneurysm can be diagnosed using two dimensional echocardiography and nuclear imaging, permitting early operative intervention before fatal rupture.", "contents": "Noninvasive diagnosis of left ventricular pseudoaneurysm: role of two dimensional echocardiography and radionuclide gated pool imaging. This report describes a case of left ventricular pseudoaneurysm diagnosed with two dimensional real time echocardiography. The two dimensional echocardiogram identified not only the pseudoaneurysmal sac but also the site of left ventricular rupture. When supplemented with radioisotope gated cardiac blood pool scanning, the noninvasive studies demonstrated combined true and false left ventricular aneurysms. Left ventricular pseudoaneurysm can be diagnosed using two dimensional echocardiography and nuclear imaging, permitting early operative intervention before fatal rupture."}
{"id": "PMID:463775", "title": "Absence of paradoxical pulse in patients with cardiac tamponade and atrial septal defects.", "content": "Five patients with atrial septal defect and proved cardiac tamponade had neither paradoxical pulse nor the inspiratory reduction of left ventricular internal dimension associated with this sign. It is assumed that equilibration of flow across the atrial septal defect prevented paradoxical pulse. Patients with a large atrial septal defect and tamponade do not manifest a paradoxical pulse.", "contents": "Absence of paradoxical pulse in patients with cardiac tamponade and atrial septal defects. Five patients with atrial septal defect and proved cardiac tamponade had neither paradoxical pulse nor the inspiratory reduction of left ventricular internal dimension associated with this sign. It is assumed that equilibration of flow across the atrial septal defect prevented paradoxical pulse. Patients with a large atrial septal defect and tamponade do not manifest a paradoxical pulse."}
{"id": "PMID:463776", "title": "Dissecting aortic aneurysm involving a right-sided aortic arch.", "content": "In this case, the first reported instance of aortic dissection involving a right-sided arch, an anomalous fourth arch vessel, the left subclavian artery, arose from a congenital aortic diverticulum. This report emphasizes the need for precise anatomic definition with aortography to permit appropriate therapy when congenital anomalies of the aortic arch are complicated by dissecting hematoma.", "contents": "Dissecting aortic aneurysm involving a right-sided aortic arch. In this case, the first reported instance of aortic dissection involving a right-sided arch, an anomalous fourth arch vessel, the left subclavian artery, arose from a congenital aortic diverticulum. This report emphasizes the need for precise anatomic definition with aortography to permit appropriate therapy when congenital anomalies of the aortic arch are complicated by dissecting hematoma."}
{"id": "PMID:463784", "title": "Ultrastructural study of granules in the corpora letea of several mammalian species.", "content": "The ultrastructure of the corpora lutea of the goat, cow, rabbit, pig and guinea pig have been examined at a stage when progesterone was being secreted by the luteal cells. Densely staining granules, 0.2micrometer in diameter, are present within the luteal cells of all the species examined. Granules were detected in the extra-cellular spaces of the corpora lutea of the goat, cow and pig, but not of the rabbit or guinea pig. The luteal cells of the various species have the same basic structure but differ in the amounts of lipid and forms of smooth endoplasmic reticulum present. A common mechanism of hormone secretion, in granule form, is postulated for all luteal cells.", "contents": "Ultrastructural study of granules in the corpora letea of several mammalian species. The ultrastructure of the corpora lutea of the goat, cow, rabbit, pig and guinea pig have been examined at a stage when progesterone was being secreted by the luteal cells. Densely staining granules, 0.2micrometer in diameter, are present within the luteal cells of all the species examined. Granules were detected in the extra-cellular spaces of the corpora lutea of the goat, cow and pig, but not of the rabbit or guinea pig. The luteal cells of the various species have the same basic structure but differ in the amounts of lipid and forms of smooth endoplasmic reticulum present. A common mechanism of hormone secretion, in granule form, is postulated for all luteal cells."}
{"id": "PMID:463785", "title": "A morphometric study quantifying the mucus content of proliferating colonic epithelial cells.", "content": "We report a quantification of the maximum mucus accumulation in proliferating rat colonic epithelial cells. The proliferative potential was determined by radioautographic study of one-hour pulse exposures to tritiated thymidine, mucous content was determined by Periodic-acid Schiff (PAS) staining. We examined 55 labeled mucous cells in 0.5- to 1-micrometer serial sections. The maximum thecal and nuclear profiles of these cells were photographed and their surface areas were determined utilizing a coordinate sensor. The data were expressed as a theca-to-nucleus (T/N) ratio. The maximum (T/N) ratio for a labeled mucous cell was 3.0. We performed a similar analysis on 22 unlabeled mucous cells from upper crypt regions and surface epithelium to derive the range of (T/N) ratios for terminally differentiated mature mucous cells. The range of (T/N) ratios from these cells was from 4.8 to 16.4. Our study shows that proliferative potential of mucous cells is determined by the interrelationship between mucus accumulation and nuclear size.", "contents": "A morphometric study quantifying the mucus content of proliferating colonic epithelial cells. We report a quantification of the maximum mucus accumulation in proliferating rat colonic epithelial cells. The proliferative potential was determined by radioautographic study of one-hour pulse exposures to tritiated thymidine, mucous content was determined by Periodic-acid Schiff (PAS) staining. We examined 55 labeled mucous cells in 0.5- to 1-micrometer serial sections. The maximum thecal and nuclear profiles of these cells were photographed and their surface areas were determined utilizing a coordinate sensor. The data were expressed as a theca-to-nucleus (T/N) ratio. The maximum (T/N) ratio for a labeled mucous cell was 3.0. We performed a similar analysis on 22 unlabeled mucous cells from upper crypt regions and surface epithelium to derive the range of (T/N) ratios for terminally differentiated mature mucous cells. The range of (T/N) ratios from these cells was from 4.8 to 16.4. Our study shows that proliferative potential of mucous cells is determined by the interrelationship between mucus accumulation and nuclear size."}
{"id": "PMID:463786", "title": "Morphology of rabbit collecting duct.", "content": "Recently the assumed structural and functional homogeneity of the collecting duct (CD) has been questioned. The objective of this study was to determine if heterogeneity occurs in luminal surface membrane structure or in cytoplasmic configuration of cells in the collecting duct or both. Straight segments of cortical and medullary CD were examined in perfusion-fixed rabbit kidneys with scanning electron microscopy (SEM), light (LM) and transmission electron microscopy (TEM). Principal cells were the most abundant cells in all CD regions; intercalated cells comprised 37% of the cell population on the cortex, 18% in the outer medulla, and less than 1% in the inner medulla. SEM revealed two surface patterns among the ciliated principal cells: 1, located in the cortex and outer medulla, with few surface microvilli, and 2, located in the inner medulla, with abundant microvilli. Intercalated cells exhibited four distinctive luminal surface configurations: I, numerous short microvilli; II, both short and elongate microvilli; III, microplicae alone; and IV, both microvilli and microplicae. Intercalated cells with patterns I and II were predominant in the cortex, while cells with patterns III and IV were most common at the corticomedullary junction. TEM confirmed that marked variation existed in cytoplasmic structures of both principal and intercalated cells. These findings may either indicate the presence of several specific types of principal and intercalated cells or reflect different functional states of the principal and intercalated cells. Regardless of their significance, their presence must be considered in studies seeking to establish precise structural-functional relationships in this region of the rabbit renal tubule.", "contents": "Morphology of rabbit collecting duct. Recently the assumed structural and functional homogeneity of the collecting duct (CD) has been questioned. The objective of this study was to determine if heterogeneity occurs in luminal surface membrane structure or in cytoplasmic configuration of cells in the collecting duct or both. Straight segments of cortical and medullary CD were examined in perfusion-fixed rabbit kidneys with scanning electron microscopy (SEM), light (LM) and transmission electron microscopy (TEM). Principal cells were the most abundant cells in all CD regions; intercalated cells comprised 37% of the cell population on the cortex, 18% in the outer medulla, and less than 1% in the inner medulla. SEM revealed two surface patterns among the ciliated principal cells: 1, located in the cortex and outer medulla, with few surface microvilli, and 2, located in the inner medulla, with abundant microvilli. Intercalated cells exhibited four distinctive luminal surface configurations: I, numerous short microvilli; II, both short and elongate microvilli; III, microplicae alone; and IV, both microvilli and microplicae. Intercalated cells with patterns I and II were predominant in the cortex, while cells with patterns III and IV were most common at the corticomedullary junction. TEM confirmed that marked variation existed in cytoplasmic structures of both principal and intercalated cells. These findings may either indicate the presence of several specific types of principal and intercalated cells or reflect different functional states of the principal and intercalated cells. Regardless of their significance, their presence must be considered in studies seeking to establish precise structural-functional relationships in this region of the rabbit renal tubule."}
{"id": "PMID:463787", "title": "Freeze-fracture demonstration of communicating junctions between interstitial cells of the pulmonary interalveolar septa.", "content": "Interstitial cells in the interalveolar septa of lungs, which are considered to be myofibroblasts, are coupled by communicating junctions.", "contents": "Freeze-fracture demonstration of communicating junctions between interstitial cells of the pulmonary interalveolar septa. Interstitial cells in the interalveolar septa of lungs, which are considered to be myofibroblasts, are coupled by communicating junctions."}
{"id": "PMID:463788", "title": "Reduced cell proliferation in fetal lung after maternal administration of pilocarpine: a scintillation autoradiographic study.", "content": "Fetuses were obtained on the 28th gestational day from pregnant New Zealand white rabbits treated daily, on the 24th through the 27th gestational day, with pilocarpine HCl, 5 mg/kg in saline, or saline alone. Lung fragments from these fetuses were incubated for two hours in medium containing 3H-thymidine. Scintillation autoradiography of 1-micrometer-thick sections of these fetal lungs revealed that the lung tissue from pilocarpine-treated fetuses had significantly lower labelled cell indices for both alveolar epithelial cells and interstitial cells. These results indicate that pilocarpine treatment promotes differentiation of immature cells in the fetal lung at the expense of cell proliferation.", "contents": "Reduced cell proliferation in fetal lung after maternal administration of pilocarpine: a scintillation autoradiographic study. Fetuses were obtained on the 28th gestational day from pregnant New Zealand white rabbits treated daily, on the 24th through the 27th gestational day, with pilocarpine HCl, 5 mg/kg in saline, or saline alone. Lung fragments from these fetuses were incubated for two hours in medium containing 3H-thymidine. Scintillation autoradiography of 1-micrometer-thick sections of these fetal lungs revealed that the lung tissue from pilocarpine-treated fetuses had significantly lower labelled cell indices for both alveolar epithelial cells and interstitial cells. These results indicate that pilocarpine treatment promotes differentiation of immature cells in the fetal lung at the expense of cell proliferation."}
{"id": "PMID:463789", "title": "The habenular nuclei of the elasmobranch \"Scyllium stellare\": myelinated perikarya.", "content": "Some habenular cells of the elasmobranch, Scyllium stellare, have a perikaryon completely enveloped by myelin of the compact type. They are found only in the left habenula and not in the right. The myelin sheath accompanies the proximal segment of the process arising from the perikaryon, in a node of Ranvier-like pattern. The identity of these myelinated cells and the functional significance of their myelination are unknown.", "contents": "The habenular nuclei of the elasmobranch \"Scyllium stellare\": myelinated perikarya. Some habenular cells of the elasmobranch, Scyllium stellare, have a perikaryon completely enveloped by myelin of the compact type. They are found only in the left habenula and not in the right. The myelin sheath accompanies the proximal segment of the process arising from the perikaryon, in a node of Ranvier-like pattern. The identity of these myelinated cells and the functional significance of their myelination are unknown."}
{"id": "PMID:463790", "title": "The Clara cell: a comparative ultrastructural study in mammals.", "content": "Clara cells in the terminal bronchoiles of mouse, rat, rabbit, calf and human were compared by light, transmission and scanning microscopy, and species-differences were clearly present. Mouse Clara cells were most numerous and mouse and rabbit Clara cells had large dense mitochondria. Rabbit and calf had glycogen in Clara cells and rat Clara cells had the most variability in secretory granules, some of which had a crystalline structure. Calf Clara cells had deeply indented nuclei. Human Clara cells had the most prominent nucleoli and lacked smooth endoplasmic reticulum, which was a prominent feature of most other species. No evidence of apical extrusion or apocrine secretion of Clara cell secretory granules was observed.", "contents": "The Clara cell: a comparative ultrastructural study in mammals. Clara cells in the terminal bronchoiles of mouse, rat, rabbit, calf and human were compared by light, transmission and scanning microscopy, and species-differences were clearly present. Mouse Clara cells were most numerous and mouse and rabbit Clara cells had large dense mitochondria. Rabbit and calf had glycogen in Clara cells and rat Clara cells had the most variability in secretory granules, some of which had a crystalline structure. Calf Clara cells had deeply indented nuclei. Human Clara cells had the most prominent nucleoli and lacked smooth endoplasmic reticulum, which was a prominent feature of most other species. No evidence of apical extrusion or apocrine secretion of Clara cell secretory granules was observed."}
{"id": "PMID:463791", "title": "Morphogenesis of the fetal membranes of an American mole, Scalopus aquaticus.", "content": "Scalopus membranes are characterized by: Superficial nidation; antimesometrial orientation of the embryonic disc; amniogenesis by folding; an extensive but transitory choriovitelline placenta; a large yolk sac with late and incomplete inversion; large persistent allantoic vesicle; a very broad, thin, villous, epitheliochorial chorioallantoic placenta of annular shape interrupted mesometrially, dotted with numerous areolae, and bordered by a nonvillous sparsely vascular chorioallantoic membrane connected with the persistent bilaminar omphalopleure by a very narrow rim of chorion. There is no decidua. Electron microscopy shows that at 8 mm, CR, (limb bud embryo) the uterine epithelium of the interhemal membrane may be 0.5 micron or less in thickness, but that it shows no signs of degeneration. Trophoblastic microvilli often penetrate the epithelium to within 0.2 micron of its base. At this time there is active secretion by the uterine glands, and cellular hypertrophy and cytolysis of the epithelium at the gland mouths, with active phagocytosis by the areolar cytotrophoblast. The occurrence of absorptive areolae in an insectivore emphasizes the probable primitiveness of this widely distributed placental mechanism. In spite of similarities of the yolk sac to that of rabbits and rodents, the bilaminar omphalopleure produces no invasive trophoblastic giant cells. The definitive membranes of Parascalops breweri and Scapanus latimanus are like those of Scalopus. The placentae of Talpa europaea, Condylura cristata, and Neurotrichus gibbsii are discoid and relatively much smaller, thicker and more complex in internal structure. There is some reason to believe that the fetal membrane systems of moles and shrews (Soricoidea) are more like those of the ancestral mammalian stock than are those of any other recent eutherians.", "contents": "Morphogenesis of the fetal membranes of an American mole, Scalopus aquaticus. Scalopus membranes are characterized by: Superficial nidation; antimesometrial orientation of the embryonic disc; amniogenesis by folding; an extensive but transitory choriovitelline placenta; a large yolk sac with late and incomplete inversion; large persistent allantoic vesicle; a very broad, thin, villous, epitheliochorial chorioallantoic placenta of annular shape interrupted mesometrially, dotted with numerous areolae, and bordered by a nonvillous sparsely vascular chorioallantoic membrane connected with the persistent bilaminar omphalopleure by a very narrow rim of chorion. There is no decidua. Electron microscopy shows that at 8 mm, CR, (limb bud embryo) the uterine epithelium of the interhemal membrane may be 0.5 micron or less in thickness, but that it shows no signs of degeneration. Trophoblastic microvilli often penetrate the epithelium to within 0.2 micron of its base. At this time there is active secretion by the uterine glands, and cellular hypertrophy and cytolysis of the epithelium at the gland mouths, with active phagocytosis by the areolar cytotrophoblast. The occurrence of absorptive areolae in an insectivore emphasizes the probable primitiveness of this widely distributed placental mechanism. In spite of similarities of the yolk sac to that of rabbits and rodents, the bilaminar omphalopleure produces no invasive trophoblastic giant cells. The definitive membranes of Parascalops breweri and Scapanus latimanus are like those of Scalopus. The placentae of Talpa europaea, Condylura cristata, and Neurotrichus gibbsii are discoid and relatively much smaller, thicker and more complex in internal structure. There is some reason to believe that the fetal membrane systems of moles and shrews (Soricoidea) are more like those of the ancestral mammalian stock than are those of any other recent eutherians."}
{"id": "PMID:463792", "title": "Ultrastructure of the renal corpuscle of the garter snake Thamnophis sirtalis.", "content": "The renal corpuscle of the garter snake was examined using scanning and transmission electron microscopy, freeze-fracture and tracer experiments. The renal corpuscle was small and glomerular architecture simple. The visceral epithelium was composed of irregular, highly branched podocytes with broad, interdigitating foot processes. Numerous cell junctions, including fasciae occludentes and adhaerentes, occurred between podocytes. The podocytes bore numerous microvilli which projected into the urinary space and contained large bundles of microfilaments. The podocytes lay on a basal lamina which appeared to act as a primary filtration barrier.", "contents": "Ultrastructure of the renal corpuscle of the garter snake Thamnophis sirtalis. The renal corpuscle of the garter snake was examined using scanning and transmission electron microscopy, freeze-fracture and tracer experiments. The renal corpuscle was small and glomerular architecture simple. The visceral epithelium was composed of irregular, highly branched podocytes with broad, interdigitating foot processes. Numerous cell junctions, including fasciae occludentes and adhaerentes, occurred between podocytes. The podocytes bore numerous microvilli which projected into the urinary space and contained large bundles of microfilaments. The podocytes lay on a basal lamina which appeared to act as a primary filtration barrier."}
{"id": "PMID:463798", "title": "Loss of body nitrogen on fasting.", "content": "An analysis of the change in total body nitrogen during fasting shows that it declines exponentially, a small fraction being lost rapidly (t 1/2 of a few days), and the remainder being lost slowly (t 1/2 of many months). The obese faster loses N, and weight, at a slower relative rate than the nonobese; and the ratio of N loss to weight loss during an extended fast is inversely related to body fat content, being about 20 g/kg in the nonobese and about 10 g/kg in those with body fat burdens of 50 kg or more. The loss of body N on a low protein-calorie adequate diet can also be described in exponential terms, and this function allows an estimate to be made of the N requirement.", "contents": "Loss of body nitrogen on fasting. An analysis of the change in total body nitrogen during fasting shows that it declines exponentially, a small fraction being lost rapidly (t 1/2 of a few days), and the remainder being lost slowly (t 1/2 of many months). The obese faster loses N, and weight, at a slower relative rate than the nonobese; and the ratio of N loss to weight loss during an extended fast is inversely related to body fat content, being about 20 g/kg in the nonobese and about 10 g/kg in those with body fat burdens of 50 kg or more. The loss of body N on a low protein-calorie adequate diet can also be described in exponential terms, and this function allows an estimate to be made of the N requirement."}
{"id": "PMID:463799", "title": "Nitrogen balance in obese patients receiving a very low calorie liquid formula diet.", "content": "Eleven obese patients were placed on a liquid formula diet containing 320 kcal (1.34 MJ), 31 g protein, 44 g oligosaccharides, 1.5 g fat, vitamins, and essential minerals for a period of 4 weeks under metabolic ward conditions. The diet was well tolerated, and mean weight loss was 2.49 kg/week during the 4-week period. Nitrogen excretion diminished in all patients during the period of treatment, but nitrogen balance remained slightly negative in most patients, mean daily deficit being 1.3 g N/day at the end of the study. The rate at which nitrogen was loss declined in a biphasic fashion, a slower second phase after an initial rapid period of adjustment to the diet. During the study a mean of 4.1 +/- 1.4% SD of calculated total body nitrogen was lost. Potassium excretion studies did not reveal significant potassium losses. The amount of nitrogen loss was correlated with the urinary creatinine excretion, suggesting that nitrogen loss during reduced dietary intake of protein is largely dependent on the size of the lean body mass.", "contents": "Nitrogen balance in obese patients receiving a very low calorie liquid formula diet. Eleven obese patients were placed on a liquid formula diet containing 320 kcal (1.34 MJ), 31 g protein, 44 g oligosaccharides, 1.5 g fat, vitamins, and essential minerals for a period of 4 weeks under metabolic ward conditions. The diet was well tolerated, and mean weight loss was 2.49 kg/week during the 4-week period. Nitrogen excretion diminished in all patients during the period of treatment, but nitrogen balance remained slightly negative in most patients, mean daily deficit being 1.3 g N/day at the end of the study. The rate at which nitrogen was loss declined in a biphasic fashion, a slower second phase after an initial rapid period of adjustment to the diet. During the study a mean of 4.1 +/- 1.4% SD of calculated total body nitrogen was lost. Potassium excretion studies did not reveal significant potassium losses. The amount of nitrogen loss was correlated with the urinary creatinine excretion, suggesting that nitrogen loss during reduced dietary intake of protein is largely dependent on the size of the lean body mass."}
{"id": "PMID:463800", "title": "The short-term effects of protein intake on 3-methylhistidine excretion.", "content": "The urinary excretion of 3-methylhistidine is used as a convenient index of muscle protein catabolism. Histidine is methylated in peptide linkage within muscle protein, and is quantitatively excreted when the protein is catabolized. 3-Methylhistidine would be expected to be present in ingested muscle protein, and unless altered in the digestive process, to be excreted as a function of intake. We studied its excretion at four levels of mixed protein intake, in separate groups of obese subjects, on the 1st day of altered input, with and without concurrent energy provision. A significant (P less than 0.001) linear relationship between protein intake and 3-methylhistidine excretion was observed, with a mean excretion of 198 mumoles/day at 0 intake, and an increment of 1.34 mumoles/g of ingested protein. There was no relationship between its excretion and the concurrent nitrogen balance. Thus, estimates of muscle protein catabolism with this method require that muscle protein be absent from the diet or its contribution quantified.", "contents": "The short-term effects of protein intake on 3-methylhistidine excretion. The urinary excretion of 3-methylhistidine is used as a convenient index of muscle protein catabolism. Histidine is methylated in peptide linkage within muscle protein, and is quantitatively excreted when the protein is catabolized. 3-Methylhistidine would be expected to be present in ingested muscle protein, and unless altered in the digestive process, to be excreted as a function of intake. We studied its excretion at four levels of mixed protein intake, in separate groups of obese subjects, on the 1st day of altered input, with and without concurrent energy provision. A significant (P less than 0.001) linear relationship between protein intake and 3-methylhistidine excretion was observed, with a mean excretion of 198 mumoles/day at 0 intake, and an increment of 1.34 mumoles/g of ingested protein. There was no relationship between its excretion and the concurrent nitrogen balance. Thus, estimates of muscle protein catabolism with this method require that muscle protein be absent from the diet or its contribution quantified."}
{"id": "PMID:463801", "title": "Increased taste thresholds of amino acids with age.", "content": "Taste detection thresholds for 19 L-amino acids and four monohydrochloride derivatives were determined for young and elderly subjects. Although the ranges of the thresholds were extensive, the elderly were found to have significantly diminished sensitivity. On the average, thresholds were 2 1/2 times higher in the elderly than in young subjects. Relationships between the taste detection thresholds of amino acids, their chemical structures, and suprathreshold taste qualities were found. Amino acids with aliphatic side chains and those containing hydroxyl groups and having sweet tastes at suprathreshold concentrations tended to have the highest thresholds. Amino acids containing sulfur, as well as those with acidic or basic groups, tended to have the lowest thresholds.", "contents": "Increased taste thresholds of amino acids with age. Taste detection thresholds for 19 L-amino acids and four monohydrochloride derivatives were determined for young and elderly subjects. Although the ranges of the thresholds were extensive, the elderly were found to have significantly diminished sensitivity. On the average, thresholds were 2 1/2 times higher in the elderly than in young subjects. Relationships between the taste detection thresholds of amino acids, their chemical structures, and suprathreshold taste qualities were found. Amino acids with aliphatic side chains and those containing hydroxyl groups and having sweet tastes at suprathreshold concentrations tended to have the highest thresholds. Amino acids containing sulfur, as well as those with acidic or basic groups, tended to have the lowest thresholds."}
{"id": "PMID:463802", "title": "Effect of high carbohydrate feeding with dextrose or sucrose on adipose tissue lipoprotein lipase activity and plasma triglyceride levels in hemodialysis patients.", "content": "The acute effect of feeding high concentration carbohydrate meals containing equicaloric amounts of dextrose or sucrose on the activity of adipose tissue lipoprotein lipase and the concentration of plasma triglyceride was assessed in 11 hemodialysis patients. Dextrose feeding resulted in higher postprandial glucose levels and a greater insulin response than sucrose. The relationship between the postprandial change in the activity of adipose tissue lipoprotein lipase and the insulin response to feeding almost reached statistical significant (rs = 0.40, P = 0.08, n = 20), and the increase in the activity of the enzyme after dextrose feeding was greater than after sucrose (P less than 0.01). There was a significant decrease in plasma triglyceride levels after dextrose feeding (P less than 0.01), but no change was observed after the ingestion of sucrose. These results indicate that the inability of the administered sucrose to raise the plasma insulin concentration to the same level as isocaloric amounts of dextrose probably accounts for the smaller increase in the activity of adipose tissue lipoprotein lipase after sucrose. The failure of plasma triglyceride levels to fall after sucrose feeding suggests that the extent to which the activity of adipose tissue lipoprotein lipase increases postprandially may be important in the regulation of triglyceride metabolism in hemodialysis patients.", "contents": "Effect of high carbohydrate feeding with dextrose or sucrose on adipose tissue lipoprotein lipase activity and plasma triglyceride levels in hemodialysis patients. The acute effect of feeding high concentration carbohydrate meals containing equicaloric amounts of dextrose or sucrose on the activity of adipose tissue lipoprotein lipase and the concentration of plasma triglyceride was assessed in 11 hemodialysis patients. Dextrose feeding resulted in higher postprandial glucose levels and a greater insulin response than sucrose. The relationship between the postprandial change in the activity of adipose tissue lipoprotein lipase and the insulin response to feeding almost reached statistical significant (rs = 0.40, P = 0.08, n = 20), and the increase in the activity of the enzyme after dextrose feeding was greater than after sucrose (P less than 0.01). There was a significant decrease in plasma triglyceride levels after dextrose feeding (P less than 0.01), but no change was observed after the ingestion of sucrose. These results indicate that the inability of the administered sucrose to raise the plasma insulin concentration to the same level as isocaloric amounts of dextrose probably accounts for the smaller increase in the activity of adipose tissue lipoprotein lipase after sucrose. The failure of plasma triglyceride levels to fall after sucrose feeding suggests that the extent to which the activity of adipose tissue lipoprotein lipase increases postprandially may be important in the regulation of triglyceride metabolism in hemodialysis patients."}
{"id": "PMID:463803", "title": "Isocaloric exchange of dietary starch and sucrose in humans. I. Effects on levels of fasting blood lipids.", "content": "For 6 weeks, 10 men and nine women aged 35 to 55 consumed each of two diets in a cross-over design. The diets were comprised of identical natural foods with 30% of the calories as either sucrose or cooked wheat starch. Carbohydrate, fat and protein supplied 43, 42, and 15% of the calories, respectively. Of the calories 10% were eaten at breakfast (7:00 to 8:30 AM) and 90% at dinner (4:30 to 6:30 PM). Initial body weights were essentially maintained. Total serum lipids, triglycerides, and total cholesterol levels were significantly higher when the subjects consumed the sucrose diet than when they consumed the starch diet. Increases associated with the sucrose diet were greatest for triglycerides (33.0%). In a subgroup of nine subjects with triglyceride levels above the normal range, sucrose feeding increased triglyceride levels 45.2%. Triglycerides and pre-beta lipoproteins were significantly higher in males than in females. Pre-beta lipoproteins were 32% higher when the subjects consumed sucrose than when they consumed starch. For alpha and beta lipoproteins, small, nonsignificant increases were associated with sucrose feeding. Serum free fatty acids were not affected by diet. These results indicate that the consumption of sucrose can increase blood lipids that are considered to be risk factors in heart disease and that males and carbohydrate-sensitive individuals may be more susceptible than others to the effects of sucrose.", "contents": "Isocaloric exchange of dietary starch and sucrose in humans. I. Effects on levels of fasting blood lipids. For 6 weeks, 10 men and nine women aged 35 to 55 consumed each of two diets in a cross-over design. The diets were comprised of identical natural foods with 30% of the calories as either sucrose or cooked wheat starch. Carbohydrate, fat and protein supplied 43, 42, and 15% of the calories, respectively. Of the calories 10% were eaten at breakfast (7:00 to 8:30 AM) and 90% at dinner (4:30 to 6:30 PM). Initial body weights were essentially maintained. Total serum lipids, triglycerides, and total cholesterol levels were significantly higher when the subjects consumed the sucrose diet than when they consumed the starch diet. Increases associated with the sucrose diet were greatest for triglycerides (33.0%). In a subgroup of nine subjects with triglyceride levels above the normal range, sucrose feeding increased triglyceride levels 45.2%. Triglycerides and pre-beta lipoproteins were significantly higher in males than in females. Pre-beta lipoproteins were 32% higher when the subjects consumed sucrose than when they consumed starch. For alpha and beta lipoproteins, small, nonsignificant increases were associated with sucrose feeding. Serum free fatty acids were not affected by diet. These results indicate that the consumption of sucrose can increase blood lipids that are considered to be risk factors in heart disease and that males and carbohydrate-sensitive individuals may be more susceptible than others to the effects of sucrose."}
{"id": "PMID:463804", "title": "Triglyceride integrated concentrations: effect of variation of source and amount of dietary carbohydrate.", "content": "The effect of changes in the amount of dietary carbohydrate (45 or 65% of total energy) and in the source of carbohydrate (sucrose or corn syrup) on plasma triglyceride and cholesterol concentrations was studied in eight healthy males. Subjects ingested each of the four formula diets for 10 days in a latin square sequence. Diet-induced response was assessed by measurement of plasma lipid concentrations in blood obtained after overnight fast and by measurement of the mean plasma lipid concentrations--designated the integrated concentration--of blood obtained by 24 hr continuous blood withdrawal. The fasting plasma triglyceride concentration increased significantly during ingestion of the high carbohydrate diet (P less than 0.005) but was not significantly influenced by the source of carbohydrate calories. The 45% carbohydrate diets induced larger meal associated plasma triglyceride variation than 65% diets. Sucrose-containing diets induced significantly higher plasma triglyceride integrated concentrations than corn syrup diets, whether provided as 45% (P less than 0.05) or 65% (P less than 0.005) of total energy. Diet-induced changes in fasting or integrated plasma cholesterol concentration were minimal.", "contents": "Triglyceride integrated concentrations: effect of variation of source and amount of dietary carbohydrate. The effect of changes in the amount of dietary carbohydrate (45 or 65% of total energy) and in the source of carbohydrate (sucrose or corn syrup) on plasma triglyceride and cholesterol concentrations was studied in eight healthy males. Subjects ingested each of the four formula diets for 10 days in a latin square sequence. Diet-induced response was assessed by measurement of plasma lipid concentrations in blood obtained after overnight fast and by measurement of the mean plasma lipid concentrations--designated the integrated concentration--of blood obtained by 24 hr continuous blood withdrawal. The fasting plasma triglyceride concentration increased significantly during ingestion of the high carbohydrate diet (P less than 0.005) but was not significantly influenced by the source of carbohydrate calories. The 45% carbohydrate diets induced larger meal associated plasma triglyceride variation than 65% diets. Sucrose-containing diets induced significantly higher plasma triglyceride integrated concentrations than corn syrup diets, whether provided as 45% (P less than 0.05) or 65% (P less than 0.005) of total energy. Diet-induced changes in fasting or integrated plasma cholesterol concentration were minimal."}
{"id": "PMID:463805", "title": "The effects of vitamin C, vitamin B6, and vitamin B12 supplementation on the breast milk and maternal status of well-nourished women.", "content": "The effects of vitamin supplements and/or diet on the levels of vitamin C, vitamin B6, and vitamin B12 in milk and blood of lactating women were determined. At the end of gestation, subjects were divided into two lactation groups: supplemented (10 subjects) and nonsupplemented (seven subjects). Milk samples were collected from 5 to 7 days and 43 to 45 days postpartum. Fasting blood samples were drawn at 8 and 46 days postpartum for vitamin C, B6, and B12 status measurements. Dietary records of all foods consumed by the subject were kept for 4 days at 1 and 6 weeks postpartum. The vitamin B6 level in breast milk of the unsupplemented group of mothers was significantly lower (P less than 0.05) than the supplemented group of women at 5 to 7 days postpartum. Vitamin B12 concentration in milk of nonsupplemented mothers at 43 to 45 days postpartum was significantly lower (P less than 0.05) than the supplemented group of women at 43 to 45 days postpartum. None of the milk values or the maternal blood levels measured in the women was less than published norms for vitamin C, vitamin B6, and vitamin B12.", "contents": "The effects of vitamin C, vitamin B6, and vitamin B12 supplementation on the breast milk and maternal status of well-nourished women. The effects of vitamin supplements and/or diet on the levels of vitamin C, vitamin B6, and vitamin B12 in milk and blood of lactating women were determined. At the end of gestation, subjects were divided into two lactation groups: supplemented (10 subjects) and nonsupplemented (seven subjects). Milk samples were collected from 5 to 7 days and 43 to 45 days postpartum. Fasting blood samples were drawn at 8 and 46 days postpartum for vitamin C, B6, and B12 status measurements. Dietary records of all foods consumed by the subject were kept for 4 days at 1 and 6 weeks postpartum. The vitamin B6 level in breast milk of the unsupplemented group of mothers was significantly lower (P less than 0.05) than the supplemented group of women at 5 to 7 days postpartum. Vitamin B12 concentration in milk of nonsupplemented mothers at 43 to 45 days postpartum was significantly lower (P less than 0.05) than the supplemented group of women at 43 to 45 days postpartum. None of the milk values or the maternal blood levels measured in the women was less than published norms for vitamin C, vitamin B6, and vitamin B12."}
{"id": "PMID:463806", "title": "The effects of ascorbic acid and flavonoids on the occurrence of symptoms normally associated with the common cold.", "content": "A controlled study was made of the effects of natural orange juice, synthetic orange juice, and placebo in the prevention of the common cold; both natural and synthetic orange juices contained 80 mg of ascorbic acid daily. Three-hundred sixty-two healthy normal young adult volunteers, ages 17 to 25 years, were studied for 72 days with 97% of participants completing the trial. There was a 14 to 21% reduction in total symptoms due to the common cold in the supplemented groups that was statistically significant (P less than 0.05). Ascorbic acid supplementation also increased the number of \"episode-free\" subjects. However, the clinical usefulness of the results does not support prophylactic ascorbic acid supplements in the well-nourished adult. The results in this study with both natural and synthetic orange juice of physiological content of ascorbic acid, are similar to those obtained using a \"megadose\" of ascorbic acid.", "contents": "The effects of ascorbic acid and flavonoids on the occurrence of symptoms normally associated with the common cold. A controlled study was made of the effects of natural orange juice, synthetic orange juice, and placebo in the prevention of the common cold; both natural and synthetic orange juices contained 80 mg of ascorbic acid daily. Three-hundred sixty-two healthy normal young adult volunteers, ages 17 to 25 years, were studied for 72 days with 97% of participants completing the trial. There was a 14 to 21% reduction in total symptoms due to the common cold in the supplemented groups that was statistically significant (P less than 0.05). Ascorbic acid supplementation also increased the number of \"episode-free\" subjects. However, the clinical usefulness of the results does not support prophylactic ascorbic acid supplements in the well-nourished adult. The results in this study with both natural and synthetic orange juice of physiological content of ascorbic acid, are similar to those obtained using a \"megadose\" of ascorbic acid."}
{"id": "PMID:463807", "title": "Integumentary loss of calcium.", "content": "Integumentary calcium loss was studied in 16 healthy young men. The daily loss by the 16 ambulatory but relatively sedentary young men in 52 determinations of 6-day periods each was 8.7 +/- 1.9 mg/m2 per day (average 15.8 mg/man per day). The amount lost was not influenced by calcium intake (0.1 to 2.3 g/day). In contrast to urinary calcium excretion, which is directly related to protein intake, there was no significant change in integumentary calcium loss with varying protein intakes (1 to 96 g nitrogen per day). No compensatory relationship between urinary and integumentary calcium excretion was noted. During strenuous exercise calcium loss increased to an average of 25 mg in 40 min. There was no compensatory decrease in urinary excretion on the day of strenuous exercise. It was also noted that integumentary calcium loss was not affected by general calcium balance.", "contents": "Integumentary loss of calcium. Integumentary calcium loss was studied in 16 healthy young men. The daily loss by the 16 ambulatory but relatively sedentary young men in 52 determinations of 6-day periods each was 8.7 +/- 1.9 mg/m2 per day (average 15.8 mg/man per day). The amount lost was not influenced by calcium intake (0.1 to 2.3 g/day). In contrast to urinary calcium excretion, which is directly related to protein intake, there was no significant change in integumentary calcium loss with varying protein intakes (1 to 96 g nitrogen per day). No compensatory relationship between urinary and integumentary calcium excretion was noted. During strenuous exercise calcium loss increased to an average of 25 mg in 40 min. There was no compensatory decrease in urinary excretion on the day of strenuous exercise. It was also noted that integumentary calcium loss was not affected by general calcium balance."}
{"id": "PMID:463809", "title": "A two-reservoir energy model of the human body.", "content": "A macroscopic two-reservoir model relating body weight, exercise, basal metabolism, and food intake is developed from the principle of the conservation of energy. A feature of this model is that it can account for changes in aqueous fluids. Experimental data on subjects undergoing a semistarvation diet are fit by the adjustment of a single parameter. The effects of changes in the activity level on the equilibrium body weight and the variation of the equilibrium body weight in individuals of different food utilization efficiencies are considered. The hypothesis that over-eating may lead initially to a volume displacement of aqueous fluids by fat is demonstrated by application of the model to experimental data; this hypothesis obviates paradoxical results suggested by single reservoir concepts.", "contents": "A two-reservoir energy model of the human body. A macroscopic two-reservoir model relating body weight, exercise, basal metabolism, and food intake is developed from the principle of the conservation of energy. A feature of this model is that it can account for changes in aqueous fluids. Experimental data on subjects undergoing a semistarvation diet are fit by the adjustment of a single parameter. The effects of changes in the activity level on the equilibrium body weight and the variation of the equilibrium body weight in individuals of different food utilization efficiencies are considered. The hypothesis that over-eating may lead initially to a volume displacement of aqueous fluids by fat is demonstrated by application of the model to experimental data; this hypothesis obviates paradoxical results suggested by single reservoir concepts."}
{"id": "PMID:463811", "title": "Compressibility of skinfolds and the measurement of subcutaneous fatness.", "content": "Compressibility of subcutaneous fat thickness when measured with skinfold calipers was investigated in 65 white American youths. Compression of skinfolds was determined relative to measurements of subcutaneous fat thickness from radiographs at each of seven sites. There is statistically significant heterogeneity among sites in skinfold compression, with skinfolds on the medial and lateral calf being the least compressible of those measured. There is little statistically significant sex difference in skinfold compression in the present sample and, within the range concerned, there were no significant correlations between skinfold compressibility and age. When compressibilities of the seven skinfolds were intercorrelated within individuals, statistically significant average correlations were obtained, indicating that individuals tend toward similar degrees of skinfold compressibility among sites. This communality of skinfold compression within individuals is such that, at least in male youth, there are significant differences among individuals in the average compressibility of the seven skinfolds.", "contents": "Compressibility of skinfolds and the measurement of subcutaneous fatness. Compressibility of subcutaneous fat thickness when measured with skinfold calipers was investigated in 65 white American youths. Compression of skinfolds was determined relative to measurements of subcutaneous fat thickness from radiographs at each of seven sites. There is statistically significant heterogeneity among sites in skinfold compression, with skinfolds on the medial and lateral calf being the least compressible of those measured. There is little statistically significant sex difference in skinfold compression in the present sample and, within the range concerned, there were no significant correlations between skinfold compressibility and age. When compressibilities of the seven skinfolds were intercorrelated within individuals, statistically significant average correlations were obtained, indicating that individuals tend toward similar degrees of skinfold compressibility among sites. This communality of skinfold compression within individuals is such that, at least in male youth, there are significant differences among individuals in the average compressibility of the seven skinfolds."}
{"id": "PMID:463812", "title": "Evidences suggestive of no intestinal nitrogen fixation for improving protein nutrition status in sweet potato eaters.", "content": "Total of seven teenagers and two adults were given sweet potato diets that supplied slightly below requirement levels of protein for 32 (experiment 1) or 53 days (experiment 2) in two separate experiments. In experiment 1 and during the first 36 days of experiment 2, nitrogen (N) balance studies with the teenagers were conducted with 0.67 and 0.71 g of protein per kilogram of body weight, respectively. Results of the N balances including skin N loss were -0.5 mg N/kg per day in experiment 1 and -3.2 mg N/kg per day in experiment 2. Two adults were given 0.63 g/kg of protein and gave average N balance of +6.0 mg N/kg per day (experiment 2), however, one of them had a slightly negative cumulative N balance if miscellaneous N losses were included in calculation. Plasma urea N of both teenagers and adults decreased significantly from 8 to 11 to 2 to 3 mg/100 ml in experiment 2. After 32 or 53 days on the sweet potato diets, the plasma free amino acid pattern of the teenager subjects showed abnormality. Furthermore, the subjects were easier to get fatigue by physical exercise, and took longer nap due to sleepiness during the latter days of the experiments. Thus, although the mean hemoglobin, hematocrit, plasma total protein, and plasma albumin levels were within the normal ranges, the protein nutrition status of the teenagers and one of the two adults was considered to be not normal. The results suggested that intestinal N-fixation that was proposed by several investigators to occur in sweet potato eaters probably did not occur. The subjects' feces were examined in vitro for the ability of N-fixation with negative results. During the last 17 days of experiment 2 the effect of excess calorie on N balance was examined.", "contents": "Evidences suggestive of no intestinal nitrogen fixation for improving protein nutrition status in sweet potato eaters. Total of seven teenagers and two adults were given sweet potato diets that supplied slightly below requirement levels of protein for 32 (experiment 1) or 53 days (experiment 2) in two separate experiments. In experiment 1 and during the first 36 days of experiment 2, nitrogen (N) balance studies with the teenagers were conducted with 0.67 and 0.71 g of protein per kilogram of body weight, respectively. Results of the N balances including skin N loss were -0.5 mg N/kg per day in experiment 1 and -3.2 mg N/kg per day in experiment 2. Two adults were given 0.63 g/kg of protein and gave average N balance of +6.0 mg N/kg per day (experiment 2), however, one of them had a slightly negative cumulative N balance if miscellaneous N losses were included in calculation. Plasma urea N of both teenagers and adults decreased significantly from 8 to 11 to 2 to 3 mg/100 ml in experiment 2. After 32 or 53 days on the sweet potato diets, the plasma free amino acid pattern of the teenager subjects showed abnormality. Furthermore, the subjects were easier to get fatigue by physical exercise, and took longer nap due to sleepiness during the latter days of the experiments. Thus, although the mean hemoglobin, hematocrit, plasma total protein, and plasma albumin levels were within the normal ranges, the protein nutrition status of the teenagers and one of the two adults was considered to be not normal. The results suggested that intestinal N-fixation that was proposed by several investigators to occur in sweet potato eaters probably did not occur. The subjects' feces were examined in vitro for the ability of N-fixation with negative results. During the last 17 days of experiment 2 the effect of excess calorie on N balance was examined."}
{"id": "PMID:463815", "title": "Postgraduate training in ambulatory pediatrics. Program evaluation.", "content": "Ambulatory pediatric, adolescent medicine, and child development fellowship programs listed in the Ambulatory Pediatric Association (APA) newsletter were surveyed to gather information about the programs for prospective fellows, to develop a descriptive summary, and to determine the extent to which programs adhere to APA guidelines. Number and types of positions offered, criteria for admission to programs, program educational objectives and activities, and career choice of graduates were examined for the 73 programs offering 132 fellowship positions in 1976 to 1977. In general, APA guidelines were followed. Programs emphasized patient care experience in contrast to health care administration, research, and teaching.", "contents": "Postgraduate training in ambulatory pediatrics. Program evaluation. Ambulatory pediatric, adolescent medicine, and child development fellowship programs listed in the Ambulatory Pediatric Association (APA) newsletter were surveyed to gather information about the programs for prospective fellows, to develop a descriptive summary, and to determine the extent to which programs adhere to APA guidelines. Number and types of positions offered, criteria for admission to programs, program educational objectives and activities, and career choice of graduates were examined for the 73 programs offering 132 fellowship positions in 1976 to 1977. In general, APA guidelines were followed. Programs emphasized patient care experience in contrast to health care administration, research, and teaching."}
{"id": "PMID:463816", "title": "Current pediatric roles in child abuse and neglect.", "content": "Many pediatricians are being called on to undertake expanded roles in the field of child abuse and neglect, whether as a practicing pediatrician, a hospital-based child abuse consultant, or as a child protection team pediatrician. The practicing pediatrician must consider the diagnosis of child abuse and neglect, confirm the diagnosis, report all suspected cases to child protective services, hospitalize any abused child who needs protection, and provide preventive services. The hospital-based child abuse consultant should provide consultation to primary physicians, report seriously injured cases for the primary physician or surgeon, provide expert medical testimony on difficult to prove cases, teach house staff and medical students about child abuse and neglect, and improve treatment services for abused children who are hospitalized. The child protection team pediatrician will usually become involved in the broader problem of improving team decision making and the interagency system that deals with child abuse and neglect.", "contents": "Current pediatric roles in child abuse and neglect. Many pediatricians are being called on to undertake expanded roles in the field of child abuse and neglect, whether as a practicing pediatrician, a hospital-based child abuse consultant, or as a child protection team pediatrician. The practicing pediatrician must consider the diagnosis of child abuse and neglect, confirm the diagnosis, report all suspected cases to child protective services, hospitalize any abused child who needs protection, and provide preventive services. The hospital-based child abuse consultant should provide consultation to primary physicians, report seriously injured cases for the primary physician or surgeon, provide expert medical testimony on difficult to prove cases, teach house staff and medical students about child abuse and neglect, and improve treatment services for abused children who are hospitalized. The child protection team pediatrician will usually become involved in the broader problem of improving team decision making and the interagency system that deals with child abuse and neglect."}
{"id": "PMID:463818", "title": "Selected chronic disease 'risk factors' in two elementary school populations. A pilot study.", "content": "Ninety-five first-grade elementary school students (aged 5 to 6 years) in two New York City area schools participated in a pilot test of a chronic disease primary prevention program stressing nutrition, antismoking, and physical activity. Program components included a health knowledge questionnaire; measurement of height, weight, skin-fold thickness, total serum cholesterol level, and blood pressure; and a modified Harvard step test of pulse rate recovery after exercise and subsequent classroom health education. The proportion of students with test values higher than specified critical levels was much greater in school M compared with school S. These measures will be repeated after one year to determine the feasibility and potential effectiveness of certain classroom health education activities.", "contents": "Selected chronic disease 'risk factors' in two elementary school populations. A pilot study. Ninety-five first-grade elementary school students (aged 5 to 6 years) in two New York City area schools participated in a pilot test of a chronic disease primary prevention program stressing nutrition, antismoking, and physical activity. Program components included a health knowledge questionnaire; measurement of height, weight, skin-fold thickness, total serum cholesterol level, and blood pressure; and a modified Harvard step test of pulse rate recovery after exercise and subsequent classroom health education. The proportion of students with test values higher than specified critical levels was much greater in school M compared with school S. These measures will be repeated after one year to determine the feasibility and potential effectiveness of certain classroom health education activities."}
{"id": "PMID:463819", "title": "Training in developmental pediatrics. How practitioners perceive the gap.", "content": "Ninety-seven randomly selected, board-certified pediatricians in five New England states were interviewed by two physicians to explore attitudes toward previous training and current sources of knowledge in developmental pediatrics. Formal training in development was rated as inadequate by 79%, of residency experience was viewed as highly valuable by only 30%, and 47% rated medical school as having no value. Although clinical experience was reported as a valuable source of knowledge by 99% of the sample, almost two thirds did not regard it as an adequate substitute for formal training. Frequent interdisciplinary communication was reported, and professional contacts were described as a highly valuable ongoing source of knowledge. Social class and size of practice did not correlate with differences in consultation patterns. A part-time longitudinal clinical experience for further education was preferred by 97%. Improved training with greater interdisciplinary content is needed.", "contents": "Training in developmental pediatrics. How practitioners perceive the gap. Ninety-seven randomly selected, board-certified pediatricians in five New England states were interviewed by two physicians to explore attitudes toward previous training and current sources of knowledge in developmental pediatrics. Formal training in development was rated as inadequate by 79%, of residency experience was viewed as highly valuable by only 30%, and 47% rated medical school as having no value. Although clinical experience was reported as a valuable source of knowledge by 99% of the sample, almost two thirds did not regard it as an adequate substitute for formal training. Frequent interdisciplinary communication was reported, and professional contacts were described as a highly valuable ongoing source of knowledge. Social class and size of practice did not correlate with differences in consultation patterns. A part-time longitudinal clinical experience for further education was preferred by 97%. Improved training with greater interdisciplinary content is needed."}
{"id": "PMID:463820", "title": "Physician's responsibility to parents after death of an infant. Beneficial outcome of a telephone call.", "content": "The effectiveness of a telephone call to the parents after the death of an infant has been assessed. Eleven parents who had received no formal contact after the death of their infant were interviewed at the hospital eight to 25 weeks (mean, 18 weeks) after than crisis. Eighteen other parents whose infants died were called three to 19 days (mean, nine days) after the death. The call lasted 26 minutes (range, 15 to 40 minutes). These parents were also interviewed at the hospital at nine to 27 weeks (mean, 15 weeks) after the death. Nine different problems were evaluated. Three of the 18 families who received a call had ten moderate or major problems compared with the 36 moderately severe or major problems in all 11 control families. This study documents the beneficial outcome of a telephone call in ameliorating emotional problems in the parents.", "contents": "Physician's responsibility to parents after death of an infant. Beneficial outcome of a telephone call. The effectiveness of a telephone call to the parents after the death of an infant has been assessed. Eleven parents who had received no formal contact after the death of their infant were interviewed at the hospital eight to 25 weeks (mean, 18 weeks) after than crisis. Eighteen other parents whose infants died were called three to 19 days (mean, nine days) after the death. The call lasted 26 minutes (range, 15 to 40 minutes). These parents were also interviewed at the hospital at nine to 27 weeks (mean, 15 weeks) after the death. Nine different problems were evaluated. Three of the 18 families who received a call had ten moderate or major problems compared with the 36 moderately severe or major problems in all 11 control families. This study documents the beneficial outcome of a telephone call in ameliorating emotional problems in the parents."}
{"id": "PMID:463821", "title": "Trisomy in the distal end of the long arm of chromosome 3. A condition clinically similar to the Cornelia de Lange syndrome.", "content": "A patient had a trisomy for the distal portion of the third chromosome. The major clinical features were failure to thrive, profound mental retardation, dysmorphic head shape, a short nose, anteverted nares, long eyelashes, synophrys, characteristic mouth, and short stature. The similarities between the clinical picture and the Cornelia de Lange syndrome are very striking.", "contents": "Trisomy in the distal end of the long arm of chromosome 3. A condition clinically similar to the Cornelia de Lange syndrome. A patient had a trisomy for the distal portion of the third chromosome. The major clinical features were failure to thrive, profound mental retardation, dysmorphic head shape, a short nose, anteverted nares, long eyelashes, synophrys, characteristic mouth, and short stature. The similarities between the clinical picture and the Cornelia de Lange syndrome are very striking."}
{"id": "PMID:463822", "title": "Toxicity of amphotericin b in children with cancer.", "content": "The pattern of amphotericin B toxicity was assessed retrospectively in a group of 20 children with cancer who had received one or more courses of the drug for treatment of systemic fungal infection. Azotemia was the most frequent complication, developing during 23 of 24 treatment courses. Other major toxic effects, in decreasing order of frequency, were anemia, hypokalemia, thrombocytopenia, and neutropenia. Infusion side effects, including drug-related fever, chills, and nausea, were also frequently seen. Seventeen of 20 patients were treated for disseminated histoplasmosis. Nineteen of 20 patients had acute leukemia. Although interaction with other agents could not be excluded, amphotericin B appeared to be the major causative agent for the toxic reactions noted. In no patient, however, was administration of amphotericin B stopped because of drug toxicity.", "contents": "Toxicity of amphotericin b in children with cancer. The pattern of amphotericin B toxicity was assessed retrospectively in a group of 20 children with cancer who had received one or more courses of the drug for treatment of systemic fungal infection. Azotemia was the most frequent complication, developing during 23 of 24 treatment courses. Other major toxic effects, in decreasing order of frequency, were anemia, hypokalemia, thrombocytopenia, and neutropenia. Infusion side effects, including drug-related fever, chills, and nausea, were also frequently seen. Seventeen of 20 patients were treated for disseminated histoplasmosis. Nineteen of 20 patients had acute leukemia. Although interaction with other agents could not be excluded, amphotericin B appeared to be the major causative agent for the toxic reactions noted. In no patient, however, was administration of amphotericin B stopped because of drug toxicity."}
{"id": "PMID:463823", "title": "Zollinger-Ellison syndrome with Marden-Walker syndrome. Association of two rare diseases in a 5-year-old girl.", "content": "A 5-year-old girl had the symptom of vomiting for four years. Gastric analysis showed elevated basal acidity unaffected by betazole stimulation. Roentgenographic examinations of the upper gastrointestinal tract showed two ulcers in the lesser curvature of the stomach. Fasting serum gastrin values were remarkably elevated and a calcium infusion test resulted in noticeable increases in serum gastrin levels. These data were consistent with the Zollinger-Ellison syndrome. In addition, the patient demonstrated such anomalies as retarded physical and mental development, kyphoscoliosis, median cleft palate, joint contracture and unusual facies due to blepharophimosis, and malformed low-set ears. These clinical features were in accord with the Marden-Walker syndrome. To our knowledge, the present case presents the first case of the combination of these two rare entities in the literature.", "contents": "Zollinger-Ellison syndrome with Marden-Walker syndrome. Association of two rare diseases in a 5-year-old girl. A 5-year-old girl had the symptom of vomiting for four years. Gastric analysis showed elevated basal acidity unaffected by betazole stimulation. Roentgenographic examinations of the upper gastrointestinal tract showed two ulcers in the lesser curvature of the stomach. Fasting serum gastrin values were remarkably elevated and a calcium infusion test resulted in noticeable increases in serum gastrin levels. These data were consistent with the Zollinger-Ellison syndrome. In addition, the patient demonstrated such anomalies as retarded physical and mental development, kyphoscoliosis, median cleft palate, joint contracture and unusual facies due to blepharophimosis, and malformed low-set ears. These clinical features were in accord with the Marden-Walker syndrome. To our knowledge, the present case presents the first case of the combination of these two rare entities in the literature."}
{"id": "PMID:463824", "title": "Familial asymmetric crying facies. Its occurrence secondary to hypoplasia of the anguli oris depressor muscles.", "content": "Asymmetric crying facies is a common neonatal problem with multiple causes. Asymmetric crying facies occurred in a newborn, secondary to familial hypoplasia of the anguli oris depressor muscle. The differential diagnosis of asymmetric crying facies should include this genetic cause.", "contents": "Familial asymmetric crying facies. Its occurrence secondary to hypoplasia of the anguli oris depressor muscles. Asymmetric crying facies is a common neonatal problem with multiple causes. Asymmetric crying facies occurred in a newborn, secondary to familial hypoplasia of the anguli oris depressor muscle. The differential diagnosis of asymmetric crying facies should include this genetic cause."}
{"id": "PMID:463833", "title": "Lead poisoning without encephalopathy. Effect of early diagnosis on neurologic and psychologic salvage.", "content": "Medical and psychological status of 166 patients previously treated for lead poisoning and of 22 sibling controls were evaluated. Maximum blood lead levels ranged from 40 to 471 microgram/dL. Eighteen patients had definite symptoms, 32 had questionable symptoms, and 116 were asymptomatic. No patients developed seizures, other neurological sequelae, or abnormal nerve conduction velocity. No statistically significant relationship was found between blood lead concentration (PbB) and subsequent intellectual function. The mean IQ of the patient cohort was 87, approximately at the 50th percentile for inner-city schoolchildren in Chicago. Detection prior to encephalopathy and prompt detoxification were effective in preventing or minimizing sequelae despite high PbBs.", "contents": "Lead poisoning without encephalopathy. Effect of early diagnosis on neurologic and psychologic salvage. Medical and psychological status of 166 patients previously treated for lead poisoning and of 22 sibling controls were evaluated. Maximum blood lead levels ranged from 40 to 471 microgram/dL. Eighteen patients had definite symptoms, 32 had questionable symptoms, and 116 were asymptomatic. No patients developed seizures, other neurological sequelae, or abnormal nerve conduction velocity. No statistically significant relationship was found between blood lead concentration (PbB) and subsequent intellectual function. The mean IQ of the patient cohort was 87, approximately at the 50th percentile for inner-city schoolchildren in Chicago. Detection prior to encephalopathy and prompt detoxification were effective in preventing or minimizing sequelae despite high PbBs."}
{"id": "PMID:463834", "title": "Nutritive value of elemental formula with reduced osmolality.", "content": "The osmolality of an elemental formula was reduced from 627 to 338 mOsm/kg H2O by replacing dextrose with corn syrup solids, reducing the content of casein hydrolysate, and replacing a portion of the medium-chain triglycerides with corn oil. In three convalescent malnourished infants, the protein quality of the formula was compared at isonitrogenous levels with that of a casein-sucrose-vegetable oil formula and was found to be at least as high: in all three nitrogen retention was higher than during a preceding casein period, and in one of the three it was also higher than during a following casein period. The levels of postprandial plasma amino acids suggested that threonine might be the first-limiting amino acid. Four severely malnourished infants received the formula as their only food during initial rehabilitation. The formula was well tolerated and supported satisfactory weight gain, linear growth, and serum protein regeneration.", "contents": "Nutritive value of elemental formula with reduced osmolality. The osmolality of an elemental formula was reduced from 627 to 338 mOsm/kg H2O by replacing dextrose with corn syrup solids, reducing the content of casein hydrolysate, and replacing a portion of the medium-chain triglycerides with corn oil. In three convalescent malnourished infants, the protein quality of the formula was compared at isonitrogenous levels with that of a casein-sucrose-vegetable oil formula and was found to be at least as high: in all three nitrogen retention was higher than during a preceding casein period, and in one of the three it was also higher than during a following casein period. The levels of postprandial plasma amino acids suggested that threonine might be the first-limiting amino acid. Four severely malnourished infants received the formula as their only food during initial rehabilitation. The formula was well tolerated and supported satisfactory weight gain, linear growth, and serum protein regeneration."}
{"id": "PMID:463835", "title": "Clinical and physiological manifestations of bronchiolitis and pneumonia. Outcome of respiratory syncytial virus.", "content": "The physiological abnormalities and clinical correlates of 32 infants consecutively hospitalized with lower respiratory tract disease from respiratory syncytial virus (RSV) were studied in an attempt to characterized the infant most at risk for the acute and long-term complications of RSV infection. Arterial oxygen saturation (Sao2) determinations were obtained daily by means of an ear oximeter. On admission all infants were hypoxemic with a mean Sao2 of 87% (range, 74% to 95%). The mean of the lowest Soa2 recorded during their hospitalization was 85.5% (range, 53% to 96%). The hypoxemia improved little during hospitalization but showed improvement three to seven weeks later. The severity of the hypoxemia correlated significantly with the duration of viral shedding, occurrence of apnea, respiratory rate, age, and percentage of immature neutrophils. Clinical severity did not correlate with the degree of hypoxemia.", "contents": "Clinical and physiological manifestations of bronchiolitis and pneumonia. Outcome of respiratory syncytial virus. The physiological abnormalities and clinical correlates of 32 infants consecutively hospitalized with lower respiratory tract disease from respiratory syncytial virus (RSV) were studied in an attempt to characterized the infant most at risk for the acute and long-term complications of RSV infection. Arterial oxygen saturation (Sao2) determinations were obtained daily by means of an ear oximeter. On admission all infants were hypoxemic with a mean Sao2 of 87% (range, 74% to 95%). The mean of the lowest Soa2 recorded during their hospitalization was 85.5% (range, 53% to 96%). The hypoxemia improved little during hospitalization but showed improvement three to seven weeks later. The severity of the hypoxemia correlated significantly with the duration of viral shedding, occurrence of apnea, respiratory rate, age, and percentage of immature neutrophils. Clinical severity did not correlate with the degree of hypoxemia."}
{"id": "PMID:463836", "title": "Transient respiratory depression of the newborn. Its occurrence after succinylcholine administration to the mother.", "content": "A baby born after a cesarean section experienced transient respiratory depression. The mother had a postsuccinylcholine apnea caused by homozygosity for the atypical allele for serum pseudocholinesterase; the baby was heterozygous for this allele and the usual allele for serum pseudocholinesterase. The possible relationship between the genotype of the baby and the respiratory difficulty at birth is raised.", "contents": "Transient respiratory depression of the newborn. Its occurrence after succinylcholine administration to the mother. A baby born after a cesarean section experienced transient respiratory depression. The mother had a postsuccinylcholine apnea caused by homozygosity for the atypical allele for serum pseudocholinesterase; the baby was heterozygous for this allele and the usual allele for serum pseudocholinesterase. The possible relationship between the genotype of the baby and the respiratory difficulty at birth is raised."}
{"id": "PMID:463837", "title": "Rose bengal sodium I 131 studies in infants with suspected biliary atresia.", "content": "Twenty-eight infants suspected of having biliary atresia were studied with radionuclide imaging and quantitation of liver activity clearance using rose bengal sodium I 131. Scanning the abdomen for the presence or absence of gut radioactivity was a sensitive indicator of complete biliary obstruction. Absence of gut radioactivity correctly predicted biliary atresia in all 11 infants proved to have that diagnosis. Biliary atresia was excluded by the presence of gut radioactivity in 13 of 17 infants with other forms of neonatal juandice. When present, gut activity was identifiable within 24 hours after injection. Serial tests improved specificity. Plotting the rate of clearance of liver activity did not aid diagnosis.", "contents": "Rose bengal sodium I 131 studies in infants with suspected biliary atresia. Twenty-eight infants suspected of having biliary atresia were studied with radionuclide imaging and quantitation of liver activity clearance using rose bengal sodium I 131. Scanning the abdomen for the presence or absence of gut radioactivity was a sensitive indicator of complete biliary obstruction. Absence of gut radioactivity correctly predicted biliary atresia in all 11 infants proved to have that diagnosis. Biliary atresia was excluded by the presence of gut radioactivity in 13 of 17 infants with other forms of neonatal juandice. When present, gut activity was identifiable within 24 hours after injection. Serial tests improved specificity. Plotting the rate of clearance of liver activity did not aid diagnosis."}
{"id": "PMID:463838", "title": "Congenital glomerulosclerosis and nephrotic syndrome in two infants. Speculations and pathogenesis.", "content": "The incidental finding of hyalinized glomeruli in otherwise normal infant kidneys is referred to as congenital glomerulosclerosis. Two infants had extensive glomerulosclerosis manifested by nephrotic syndrome, severe oliguria, and progressive renal failure. Both patients were believed to have had intrauterine infections. These two cases have unequivocally identified congenital glomerulosclerosis as one of the causes of nephrotic syndrome in infancy. In addition, they suggest that extensive glomerulosclerosis in some cases may be a result of congenital infections.", "contents": "Congenital glomerulosclerosis and nephrotic syndrome in two infants. Speculations and pathogenesis. The incidental finding of hyalinized glomeruli in otherwise normal infant kidneys is referred to as congenital glomerulosclerosis. Two infants had extensive glomerulosclerosis manifested by nephrotic syndrome, severe oliguria, and progressive renal failure. Both patients were believed to have had intrauterine infections. These two cases have unequivocally identified congenital glomerulosclerosis as one of the causes of nephrotic syndrome in infancy. In addition, they suggest that extensive glomerulosclerosis in some cases may be a result of congenital infections."}
{"id": "PMID:463839", "title": "Noonan's syndrome. IQ and specific disabilities.", "content": "The IQ of eight male patients with Noonan's syndrome, aged 13 to 26 years, ranged from 64 to 127, with a median of 102. The full IQ masked the possible presence of specific verbal or praxic (visual-constructional) disability. There was no verbal/praxic disparity in three cases; there was substantial verbal disability in one case; and there was major praxic disability in four cases, three unrelated to vision and one accounted for by severe visual impairment. Ostensible general mental retardation in Noonan's syndrome should always be further evaluated for specific disability. Academic placement should then be arranged accordingly.", "contents": "Noonan's syndrome. IQ and specific disabilities. The IQ of eight male patients with Noonan's syndrome, aged 13 to 26 years, ranged from 64 to 127, with a median of 102. The full IQ masked the possible presence of specific verbal or praxic (visual-constructional) disability. There was no verbal/praxic disparity in three cases; there was substantial verbal disability in one case; and there was major praxic disability in four cases, three unrelated to vision and one accounted for by severe visual impairment. Ostensible general mental retardation in Noonan's syndrome should always be further evaluated for specific disability. Academic placement should then be arranged accordingly."}
{"id": "PMID:463840", "title": "Coagulation studies in infants of diabetic mothers.", "content": "Coagulation studies on 17 infants of diabetic mothers were compared with 11 control infants. Only minor differences were noted, including lower platelet counts in the former group. On the basis of these findings, the increased thrombotic tendency in diabetic progeny cannot be attributed to altered hemostasis.", "contents": "Coagulation studies in infants of diabetic mothers. Coagulation studies on 17 infants of diabetic mothers were compared with 11 control infants. Only minor differences were noted, including lower platelet counts in the former group. On the basis of these findings, the increased thrombotic tendency in diabetic progeny cannot be attributed to altered hemostasis."}
{"id": "PMID:463844", "title": "Surgical management of ileosigmoid fistula in Crohn's disease.", "content": "Seventeen cases with Crohn's disease and ileosigmoid fistula were reviewed. They have been subdivided into four groups according to the anatomic site and extent of disease. Seven patients had disease localized to the terminal ileum and a fistula to normal sigmoid. Five were treated by ileocolic resection and simple of the sigmoid fistula (group I. All seven cases with concomitant sigmoid involvement had a double resection (group II). Two patients with diffuse ileocolitis (group III) had subtotal colectomy and ileostomy. One case (group IV) had left-sided colonic disease with a fistula to normal ileum and was treated with a double resection. In six patients, a temporary ileostomy was performed to protect the anastomoses. Forty-seven percent of patients had granulomatous involvement at only one end of the fistula. Resection of the diseases bowel and simple closure of the fistula can be performed safely in most patients in this group.", "contents": "Surgical management of ileosigmoid fistula in Crohn's disease. Seventeen cases with Crohn's disease and ileosigmoid fistula were reviewed. They have been subdivided into four groups according to the anatomic site and extent of disease. Seven patients had disease localized to the terminal ileum and a fistula to normal sigmoid. Five were treated by ileocolic resection and simple of the sigmoid fistula (group I. All seven cases with concomitant sigmoid involvement had a double resection (group II). Two patients with diffuse ileocolitis (group III) had subtotal colectomy and ileostomy. One case (group IV) had left-sided colonic disease with a fistula to normal ileum and was treated with a double resection. In six patients, a temporary ileostomy was performed to protect the anastomoses. Forty-seven percent of patients had granulomatous involvement at only one end of the fistula. Resection of the diseases bowel and simple closure of the fistula can be performed safely in most patients in this group."}
{"id": "PMID:463845", "title": "Responses of serum gastrin and gastric acid output before and after bilioenteric by-pass in the dog and man.", "content": "Responses of serum gastrin and gastric acid output levels were studied in four dogs before and after a bilioenteric by-pass. Serum gastrin levels during bombesin infusion were measured in eight patients submitted to Roux-en-Y hepatocholangiojejunostomy. No change was observed in acid secretion from the main stomach or from the Heidenhain pouch in dogs following biliojejunostomy. The peak acid output, however, occurred significantly earlier after diversion of bile from the duodenum. Serum gastrin levels decreased significantly in dogs after bilioenteric by-pass and in the operated patients compared with normal subjects. The possible role played by bile in the release of duodenal gastrin is hypothesized.", "contents": "Responses of serum gastrin and gastric acid output before and after bilioenteric by-pass in the dog and man. Responses of serum gastrin and gastric acid output levels were studied in four dogs before and after a bilioenteric by-pass. Serum gastrin levels during bombesin infusion were measured in eight patients submitted to Roux-en-Y hepatocholangiojejunostomy. No change was observed in acid secretion from the main stomach or from the Heidenhain pouch in dogs following biliojejunostomy. The peak acid output, however, occurred significantly earlier after diversion of bile from the duodenum. Serum gastrin levels decreased significantly in dogs after bilioenteric by-pass and in the operated patients compared with normal subjects. The possible role played by bile in the release of duodenal gastrin is hypothesized."}
{"id": "PMID:463846", "title": "Isolated granulomatous disease of the stomach. Report of three cases presenting as incidental findings in gastrectomy specimens.", "content": "Three cases of isolated granulomatous disease of the stomach are reported. Other granulomatous diseases were ruled out on the basis of clinical and histological studies. These cases differ from those previously described in having only mucosal granulomas which were discovered as incidental findings in stomachs removed for peptic ulcers. Perigastric lymph node involvement by granulomas was present in one of three cases.", "contents": "Isolated granulomatous disease of the stomach. Report of three cases presenting as incidental findings in gastrectomy specimens. Three cases of isolated granulomatous disease of the stomach are reported. Other granulomatous diseases were ruled out on the basis of clinical and histological studies. These cases differ from those previously described in having only mucosal granulomas which were discovered as incidental findings in stomachs removed for peptic ulcers. Perigastric lymph node involvement by granulomas was present in one of three cases."}
{"id": "PMID:463847", "title": "Tuberculosis of the duodenum.", "content": "At present, tuberculosis of the intestinal tract is rare in the United States. When gastrointestinal disease is seen, 85--90% of cases involve the ileocecal area. A case of tuberculosis of the duodenum unassociated with pulmonary disease is described. Although fiberoptic enndoscopy, abdominal ultrasonography and computerized axial tomography were used, the diagnosis required laprotomy with biopsy. The clinical manifestations, diagnostic investigations and treatment of the 49 previously reported cases of duodenal tuberculosis are also reviewed.", "contents": "Tuberculosis of the duodenum. At present, tuberculosis of the intestinal tract is rare in the United States. When gastrointestinal disease is seen, 85--90% of cases involve the ileocecal area. A case of tuberculosis of the duodenum unassociated with pulmonary disease is described. Although fiberoptic enndoscopy, abdominal ultrasonography and computerized axial tomography were used, the diagnosis required laprotomy with biopsy. The clinical manifestations, diagnostic investigations and treatment of the 49 previously reported cases of duodenal tuberculosis are also reviewed."}
{"id": "PMID:463848", "title": "Specificity of serum amylase and amylase creatinine clearance ratio in the diagnosis of acute and chronic pancreatitis.", "content": "In 31 patients with pancreatitis, the amylase to creatinine clearance ratio (CACR) was significantly greater than for controls (10.7 +/- 1.7 vs. 2.6 +/- 0.3, P less than .001). Sixteen pancreatitis patients with serum amylase (SAm) within the normal range had a mean CACR significantly greater than that of 19 hospital control patients with normal SAm (9.2 +/- 1.5 vs. 3.0 +/- 0.4, P less than .001). For control patients a highly significant inverse correlation between SAm and CACR was observed. No relationship was detected between these parameters for pancreatitis patients. The results suggest that the CACR may be of aid in establishing the diagnosis of pancreatitis even in patients without hyperamylasemia.", "contents": "Specificity of serum amylase and amylase creatinine clearance ratio in the diagnosis of acute and chronic pancreatitis. In 31 patients with pancreatitis, the amylase to creatinine clearance ratio (CACR) was significantly greater than for controls (10.7 +/- 1.7 vs. 2.6 +/- 0.3, P less than .001). Sixteen pancreatitis patients with serum amylase (SAm) within the normal range had a mean CACR significantly greater than that of 19 hospital control patients with normal SAm (9.2 +/- 1.5 vs. 3.0 +/- 0.4, P less than .001). For control patients a highly significant inverse correlation between SAm and CACR was observed. No relationship was detected between these parameters for pancreatitis patients. The results suggest that the CACR may be of aid in establishing the diagnosis of pancreatitis even in patients without hyperamylasemia."}
{"id": "PMID:463851", "title": "A case of meglumine iodipamide hepatotoxicity.", "content": "There have been only two reports of severe hepatotoxic reaction caused by meglumine iodipamide. Lately we experienced such a reaction in an 66-year old female with chronic intrahepatic cholestasis. After drip infusion cholangiography was performed by infusing 40 ml. of 50% meglumine iodipamide (Biligrafin) intravenously, the patient developed nausea and abdominal pain. Her serum transaminase rose to more than 2,000 K-A units on the third day and gradually returned to normal by the 18th day. The macrophage migration inhibition test of her blood was positive for meglumine iodipamide. Accordingly some delayed type of hypersensitivity in the above reaction could be considered. When a larger amount than a recommended dose of meglumine iodipamide is infused in cholangiography, a severe hepatotoxic reaction might be induced, especially in icteric cases.", "contents": "A case of meglumine iodipamide hepatotoxicity. There have been only two reports of severe hepatotoxic reaction caused by meglumine iodipamide. Lately we experienced such a reaction in an 66-year old female with chronic intrahepatic cholestasis. After drip infusion cholangiography was performed by infusing 40 ml. of 50% meglumine iodipamide (Biligrafin) intravenously, the patient developed nausea and abdominal pain. Her serum transaminase rose to more than 2,000 K-A units on the third day and gradually returned to normal by the 18th day. The macrophage migration inhibition test of her blood was positive for meglumine iodipamide. Accordingly some delayed type of hypersensitivity in the above reaction could be considered. When a larger amount than a recommended dose of meglumine iodipamide is infused in cholangiography, a severe hepatotoxic reaction might be induced, especially in icteric cases."}
{"id": "PMID:463852", "title": "Pancreatic pseudocyst simulating an intrahepatic mass.", "content": "A case of pancreatic pseudocyst is presented which mimicked an intrahepatic mass both after selective hepatic arteriography and isotope (99m Tc-S-Co) evaluation of the liver. A previous upper gastrointestinal series and ultrasonograhpy had seemed to accurately establish the diagnosis of pancreatic pseudocyst. A pancreatic pseudocyst may pose as an intrahepatic mass lesion and this entity should be recognized as a pitfall in nuclear imaging of the liver.", "contents": "Pancreatic pseudocyst simulating an intrahepatic mass. A case of pancreatic pseudocyst is presented which mimicked an intrahepatic mass both after selective hepatic arteriography and isotope (99m Tc-S-Co) evaluation of the liver. A previous upper gastrointestinal series and ultrasonograhpy had seemed to accurately establish the diagnosis of pancreatic pseudocyst. A pancreatic pseudocyst may pose as an intrahepatic mass lesion and this entity should be recognized as a pitfall in nuclear imaging of the liver."}
{"id": "PMID:463853", "title": "Caroli's disease. Clinical presentation simulating carcinoma of the pancreas.", "content": "A patient with Caroli's disease (intrahepatic biliary duct dilatation), presenting when he was middle-aged with symptoms of painless jaundice and weight loss suggesting carcinoma of the head of the pancreas, is described. The world's literature of Caroli's disease is reviewed and this patient's unusual presentation is put into perspective.", "contents": "Caroli's disease. Clinical presentation simulating carcinoma of the pancreas. A patient with Caroli's disease (intrahepatic biliary duct dilatation), presenting when he was middle-aged with symptoms of painless jaundice and weight loss suggesting carcinoma of the head of the pancreas, is described. The world's literature of Caroli's disease is reviewed and this patient's unusual presentation is put into perspective."}
{"id": "PMID:463854", "title": "Two cases of complicated intestinal lipoma. Review of small intestinal lipomas.", "content": "Two cases of solitary small intestinal lipoma are presented. One occured in the duodenum as a submucosal pedunculated polyp with a bleeding ulcerated surface. This was diagnosed by means of the fiberoptic duodenoscope (Olympus D1G2). The second involved a large massive submucosal lipoma which formed the leading edge of an intussusception resulting in small bowel obstruction. A brief review of the pathology, complications, symptoms, radiographic diagnosis and treatment of small intestinal lipomas is given.", "contents": "Two cases of complicated intestinal lipoma. Review of small intestinal lipomas. Two cases of solitary small intestinal lipoma are presented. One occured in the duodenum as a submucosal pedunculated polyp with a bleeding ulcerated surface. This was diagnosed by means of the fiberoptic duodenoscope (Olympus D1G2). The second involved a large massive submucosal lipoma which formed the leading edge of an intussusception resulting in small bowel obstruction. A brief review of the pathology, complications, symptoms, radiographic diagnosis and treatment of small intestinal lipomas is given."}
{"id": "PMID:463855", "title": "Transient subcutaneous granulomatosis of the upper extremities in Crohn's disease.", "content": "Skin lesions occur fairly commonly in patients with Crohn's disease of the colon (14%). Erythema nodosum and pyoderma gangrenosum are the most frequently recognized. Granulomatosis of the skin, continuous with bowel involvement, has been reported. Involvement of the subcutaneous tissue, discontinuous with intestinal disease, is unusual and we wish to report a case of Crohn's disease of the colon complicated by infraepidermal perivascular granulomas of the forearms. The lesions disappeared spontaneously.", "contents": "Transient subcutaneous granulomatosis of the upper extremities in Crohn's disease. Skin lesions occur fairly commonly in patients with Crohn's disease of the colon (14%). Erythema nodosum and pyoderma gangrenosum are the most frequently recognized. Granulomatosis of the skin, continuous with bowel involvement, has been reported. Involvement of the subcutaneous tissue, discontinuous with intestinal disease, is unusual and we wish to report a case of Crohn's disease of the colon complicated by infraepidermal perivascular granulomas of the forearms. The lesions disappeared spontaneously."}
{"id": "PMID:463857", "title": "The radiology corner. Segmental lymphangiectasia of the small bowel.", "content": "A case of intestinal lymphangiectasia, limited to a small segment of jejunum, is reported. This finding reflects part of the spectrum of generalized dysplasia of the lymphatic system. A discussion of the pathogenesis, pathophysiology and roentgen features of intestinal lymphangiectasia as part of a systemic disorder is presented.", "contents": "The radiology corner. Segmental lymphangiectasia of the small bowel. A case of intestinal lymphangiectasia, limited to a small segment of jejunum, is reported. This finding reflects part of the spectrum of generalized dysplasia of the lymphatic system. A discussion of the pathogenesis, pathophysiology and roentgen features of intestinal lymphangiectasia as part of a systemic disorder is presented."}
{"id": "PMID:463858", "title": "An outbreak of influenza aboard a commercial airliner.", "content": "A jet airliner with 54 persons aboard was delayed on the ground for three hours because of engine failure during a takeoff attempt. Most passengers stayed on the airplane during the delay. Within 72 hours, 72 per cent of the passengers became ill with symptoms of cough, fever, fatigue, headache, sore throat and myalgia. One passenger, the apparent index case, was ill on the airplane, and the clinical attack rate among the others varied with the amount of time spent aboard. Virus antigenically similar to A/Texas/1/77(H3N2) was isolated from 8 of 31 passengers cultured, and 20 of 22 ill persons tested had serologic evidence of infection with this virus. The airplane ventilation system was inoperative during the delay and this may account for the high attack rate.", "contents": "An outbreak of influenza aboard a commercial airliner. A jet airliner with 54 persons aboard was delayed on the ground for three hours because of engine failure during a takeoff attempt. Most passengers stayed on the airplane during the delay. Within 72 hours, 72 per cent of the passengers became ill with symptoms of cough, fever, fatigue, headache, sore throat and myalgia. One passenger, the apparent index case, was ill on the airplane, and the clinical attack rate among the others varied with the amount of time spent aboard. Virus antigenically similar to A/Texas/1/77(H3N2) was isolated from 8 of 31 passengers cultured, and 20 of 22 ill persons tested had serologic evidence of infection with this virus. The airplane ventilation system was inoperative during the delay and this may account for the high attack rate."}
{"id": "PMID:463860", "title": "Effect of parental cigarette smoking on the pulmonary function of children.", "content": "The authors have investigated the effects of parental smoking patterns on the pulmonary function of children in East Boston, Massachusetts. A crude inverse dose-response relationship was observed between the level of FEF25--75% predicted of children who never smoked and the number of smoking parents in the household. Compared to children with two non-smoking parents, the level of FEF25--75% predicted was 0.156 and 0.355 standard deviation units lower for children with one and two currently smoking parents, respectively. An additional decline in level of FEF25--75% predicted was observed for children who themselves had smoked. Smoking children with two smoking parents had an average FEF25--75% predicted level which was 0.355 standard deviation units lower than non-smoking children with two smoking parents. These data not only confirm that cigarette smoking by young children and teenagers has direct measurable effects on their pulmonary function, but also show that cigarette smoking by parents has a measurable effect on the pulmonary function of their children which is independent of any direct use of cigarettes by the children.", "contents": "Effect of parental cigarette smoking on the pulmonary function of children. The authors have investigated the effects of parental smoking patterns on the pulmonary function of children in East Boston, Massachusetts. A crude inverse dose-response relationship was observed between the level of FEF25--75% predicted of children who never smoked and the number of smoking parents in the household. Compared to children with two non-smoking parents, the level of FEF25--75% predicted was 0.156 and 0.355 standard deviation units lower for children with one and two currently smoking parents, respectively. An additional decline in level of FEF25--75% predicted was observed for children who themselves had smoked. Smoking children with two smoking parents had an average FEF25--75% predicted level which was 0.355 standard deviation units lower than non-smoking children with two smoking parents. These data not only confirm that cigarette smoking by young children and teenagers has direct measurable effects on their pulmonary function, but also show that cigarette smoking by parents has a measurable effect on the pulmonary function of their children which is independent of any direct use of cigarettes by the children."}
{"id": "PMID:463861", "title": "Identifying a susceptible subgroup: effects of the Pittsburgh air pollution episode upon schoolchildren.", "content": "Pulmonary function test results on 224 parochial schoolchildren collected during and after the Pittsburgh air pollution episode of November 1975 were reanalyzed to determine whether a small subgroup of susceptible children could be defined. Individual regressions of three-quarter second forced expiratory volumes (FEV.75) and forced vital capacities (FVC) on time over the six-day study period were calculated, and the distributions of individual slopes for the four exposed and two control schools were compared. Excesses of strong upward trends in the exposed areas would suggest effects of suspended particulate air pollution by indicating significant improvement following the episode. A highly statistically significant excess of strong upward trends in the FVC among exposed students was observed, and was consistent by sex and by school within sex. Approximately 10--15% of the students appear susceptible to an average impairment of about 20% of the FVC. The findings are limited by the small number of subjects with strong post-episode upward trends in the FVC, and by lack of validation by replication of the study design, but do suggest that episode levels of suspended particulates induce lung damage, and that this may occur only in a small susceptible subgroup. Children with low baseline pulmonary function values, histories of asthma, or with acute respiratory symptoms immediately following the episode were not found to be especially susceptible to these effects of suspended particulates.", "contents": "Identifying a susceptible subgroup: effects of the Pittsburgh air pollution episode upon schoolchildren. Pulmonary function test results on 224 parochial schoolchildren collected during and after the Pittsburgh air pollution episode of November 1975 were reanalyzed to determine whether a small subgroup of susceptible children could be defined. Individual regressions of three-quarter second forced expiratory volumes (FEV.75) and forced vital capacities (FVC) on time over the six-day study period were calculated, and the distributions of individual slopes for the four exposed and two control schools were compared. Excesses of strong upward trends in the exposed areas would suggest effects of suspended particulate air pollution by indicating significant improvement following the episode. A highly statistically significant excess of strong upward trends in the FVC among exposed students was observed, and was consistent by sex and by school within sex. Approximately 10--15% of the students appear susceptible to an average impairment of about 20% of the FVC. The findings are limited by the small number of subjects with strong post-episode upward trends in the FVC, and by lack of validation by replication of the study design, but do suggest that episode levels of suspended particulates induce lung damage, and that this may occur only in a small susceptible subgroup. Children with low baseline pulmonary function values, histories of asthma, or with acute respiratory symptoms immediately following the episode were not found to be especially susceptible to these effects of suspended particulates."}
{"id": "PMID:463862", "title": "Temporal and regional variation in hysterectomy rates in the United States, 1970--1975.", "content": "Regional hysterectomy rates in the United States for 1970 and 1975 have been estimated from a one per cent sample of hospital discharges. All rates have been corrected for the number of women truly at risk, that is, with uteri intact, and regional and age-specific estimates of uterine prevalence in 1975 are provided. The rates rose by one-third in 1970-1975, with the increase taking place nearly uniformly over all ages below 65 years. Rates were greatest for 40--44-year-old women, but in 1970 there was a secondary peak for women between the ages of 65 and 69 years. That peak disappeared with rising rates for younger women in 1975. For women under age 35 years, the hysterectomy rate in the South was three times higher than that in the Northeast.", "contents": "Temporal and regional variation in hysterectomy rates in the United States, 1970--1975. Regional hysterectomy rates in the United States for 1970 and 1975 have been estimated from a one per cent sample of hospital discharges. All rates have been corrected for the number of women truly at risk, that is, with uteri intact, and regional and age-specific estimates of uterine prevalence in 1975 are provided. The rates rose by one-third in 1970-1975, with the increase taking place nearly uniformly over all ages below 65 years. Rates were greatest for 40--44-year-old women, but in 1970 there was a secondary peak for women between the ages of 65 and 69 years. That peak disappeared with rising rates for younger women in 1975. For women under age 35 years, the hysterectomy rate in the South was three times higher than that in the Northeast."}
{"id": "PMID:463863", "title": "Cancer of the corpus uteri: increasing incidence in the United States, 1970--1975.", "content": "Incidence rates of cancer of the corpus uteri have been estimated from a one per cent sample of hospital discharges in the United States for the years 1970--1975. Among postmenopausal women, rates rose sharply in 1970--1975, while for younger women there was little net change. There were substantial regional variations in incidence, which were largest between the ages of 65 and 69 years. All rates have been corrected for the numbers of women in each region and age group with uteri intact.", "contents": "Cancer of the corpus uteri: increasing incidence in the United States, 1970--1975. Incidence rates of cancer of the corpus uteri have been estimated from a one per cent sample of hospital discharges in the United States for the years 1970--1975. Among postmenopausal women, rates rose sharply in 1970--1975, while for younger women there was little net change. There were substantial regional variations in incidence, which were largest between the ages of 65 and 69 years. All rates have been corrected for the numbers of women in each region and age group with uteri intact."}
{"id": "PMID:463864", "title": "Work activity and fatal heart attack studied by multiple logistic risk analysis.", "content": "A group of 3975 San Francisco longshoremen in cohorts classified annually by work activity (WA) was followed for fatal heart attack (FHA) over a 22-year period. In 57,632 person-years of follow-up, 410 men died from heart attack. A multi-factor logistic analysis was used to study work energy output adjusted for age, race, systolic blood pressure, smoking, body mass index, glucose intolerance and EKG status as predictors of FHA. After adjustment for these factors, men with a high WA of 7 kcal/min above basal metabolism at the beginning of a yearly follow-up period had a FHA rate about one-half the rate for men in the lowest WA category of 1 kcal/min above basal metabolism (p = 0.0003). In addition, the predictability of average WA during the four years preceding a yearly follow-up period was isolated to see if the lower risk associated with high WA derives from selective factors. After adjustment for factors described above and also for rate of change in WA used to represent selective factors, subjects with WA of 7 kcal/min still had about one-half the FHA rate observed for men at the lowest WA level (p = 0.0006). The findings from these and other phases of the analysis are consistent with the hypothesis that a substantial protective effect against FHA results from vigorous physical exertion.", "contents": "Work activity and fatal heart attack studied by multiple logistic risk analysis. A group of 3975 San Francisco longshoremen in cohorts classified annually by work activity (WA) was followed for fatal heart attack (FHA) over a 22-year period. In 57,632 person-years of follow-up, 410 men died from heart attack. A multi-factor logistic analysis was used to study work energy output adjusted for age, race, systolic blood pressure, smoking, body mass index, glucose intolerance and EKG status as predictors of FHA. After adjustment for these factors, men with a high WA of 7 kcal/min above basal metabolism at the beginning of a yearly follow-up period had a FHA rate about one-half the rate for men in the lowest WA category of 1 kcal/min above basal metabolism (p = 0.0003). In addition, the predictability of average WA during the four years preceding a yearly follow-up period was isolated to see if the lower risk associated with high WA derives from selective factors. After adjustment for factors described above and also for rate of change in WA used to represent selective factors, subjects with WA of 7 kcal/min still had about one-half the FHA rate observed for men at the lowest WA level (p = 0.0006). The findings from these and other phases of the analysis are consistent with the hypothesis that a substantial protective effect against FHA results from vigorous physical exertion."}
{"id": "PMID:463865", "title": "Antibody against hepatitis A in seven European countries. I. Comparison of prevalence data in different age groups.", "content": "Using a solid phase radioimmunoassay, antibody to hepatitis A virus (anti-HAV) was determined in 3890 sera from populations in seven European countries. Prevalence of anti-HAV was lowest in Scandinavian countries and highest in Greece and France. Antibodies were found in 77 (13%) of 602 blood donors in Sweden, in 29 (17%) of 175 blood donors and women taking birth control pills in Norway, in 273 (39%) of 700 blood donors in Switzerland, in 262 (52%) of 505 blood donors in Holland, in 365 (55%) of 661 accident patients in West Germany, in 452 (75%) of 600 blood donors in France and in 530 (82%) of 647 persons in Greece. Prevalence of anti-HAV increased with age in all populations tested, indicating nearly total exposure to HAV in persons over 19 years of age in Greece and in persons over 39 years of age in West Germany, Holland and France. Antibody was found more frequently in rural than in urban populations in Greece and Switzerland. Calculation of the age-specific incidence of HAV infections suggests a remarkable decline in the exposure rate in the last few decades.", "contents": "Antibody against hepatitis A in seven European countries. I. Comparison of prevalence data in different age groups. Using a solid phase radioimmunoassay, antibody to hepatitis A virus (anti-HAV) was determined in 3890 sera from populations in seven European countries. Prevalence of anti-HAV was lowest in Scandinavian countries and highest in Greece and France. Antibodies were found in 77 (13%) of 602 blood donors in Sweden, in 29 (17%) of 175 blood donors and women taking birth control pills in Norway, in 273 (39%) of 700 blood donors in Switzerland, in 262 (52%) of 505 blood donors in Holland, in 365 (55%) of 661 accident patients in West Germany, in 452 (75%) of 600 blood donors in France and in 530 (82%) of 647 persons in Greece. Prevalence of anti-HAV increased with age in all populations tested, indicating nearly total exposure to HAV in persons over 19 years of age in Greece and in persons over 39 years of age in West Germany, Holland and France. Antibody was found more frequently in rural than in urban populations in Greece and Switzerland. Calculation of the age-specific incidence of HAV infections suggests a remarkable decline in the exposure rate in the last few decades."}
{"id": "PMID:463866", "title": "Hypertension among Aleuts.", "content": "Surveys of Aleuts on St. Paul Island, Alaska, in 1966 and 1976 found a prevalence of hypertension as high as any reported in the United States. The rate remained high over the 10-year period. Preliminary data from other Aleut villages on the Aleutian Chain indicate that a high prevalence of hypertension may be widespread in this region. Etiologically genetic factors, obesity, and a high salt intake are all present. In addition, an exceedingly high level of cadmium content was found in seal livers, a dietary staple. Isolated locations such as St. Paul provide natural laboratories for possibly elucidating the complex etiology of this disease.", "contents": "Hypertension among Aleuts. Surveys of Aleuts on St. Paul Island, Alaska, in 1966 and 1976 found a prevalence of hypertension as high as any reported in the United States. The rate remained high over the 10-year period. Preliminary data from other Aleut villages on the Aleutian Chain indicate that a high prevalence of hypertension may be widespread in this region. Etiologically genetic factors, obesity, and a high salt intake are all present. In addition, an exceedingly high level of cadmium content was found in seal livers, a dietary staple. Isolated locations such as St. Paul provide natural laboratories for possibly elucidating the complex etiology of this disease."}
{"id": "PMID:463867", "title": "Laterality of breast cancer in families.", "content": "A new method for analyzing concordance for a binary variable in extended pedigrees was developed to study tumor laterality in families with high incidence of breast cancer. It was found that related breast cancer patients in 15 high-risk Midwestern US families did not have their tumors on the same side more frequently than would be expected by chance. This finding is in contrast to previous studies in London and Denmark that reported a significant concordance for tumor laterality in related breast cancer patients. This result may reflect an increasing incidence of sporadic breast cancer in genetically susceptible families or, alternatively, independent determination of cancer susceptibility and tumor laterality.", "contents": "Laterality of breast cancer in families. A new method for analyzing concordance for a binary variable in extended pedigrees was developed to study tumor laterality in families with high incidence of breast cancer. It was found that related breast cancer patients in 15 high-risk Midwestern US families did not have their tumors on the same side more frequently than would be expected by chance. This finding is in contrast to previous studies in London and Denmark that reported a significant concordance for tumor laterality in related breast cancer patients. This result may reflect an increasing incidence of sporadic breast cancer in genetically susceptible families or, alternatively, independent determination of cancer susceptibility and tumor laterality."}
{"id": "PMID:463869", "title": "Guillain-Barre syndrome following vaccination in the National Influenza Immunization Program, United States, 1976--1977.", "content": "Because of an increase in the number of reports of Guillian-Barre syndrome (GBS) following A/New Jersey influenza vaccination, the National Influenza Immunization Program was suspended December 16, 1976 and nationwide surveillance for GBS was begun. This surveillance uncovered a total of 1098 patients with onset of GBS from October 1, 1976, to January 31, 1977, from all 50 states, District of Columbia, and Puerto Rico. A total of 532 patients had recently received an A/New Jersey influenza vaccination prior to their onset of GBS (vaccinated cases), and 15 patients received a vaccination after their onset of GBS. Five hundred forty-three patients had not been recently vaccinated with A/New Jersey influenza vaccine and the vaccination status for 8 was unknown. Epidemiologic evidence indicated that many cases of GBS were related to vaccination. When compared to the unvaccinated population, the vaccinated population had a significantly elevated attack rate in every adult age group. The estimated attributable risk of vaccine-related GBS in the adult population was just under one case per 100,000 vaccinations. The period of increased risk was concentrated primarily within the 5-week period after vaccination, although it lasted for approximately 9 or 10 weeks.", "contents": "Guillain-Barre syndrome following vaccination in the National Influenza Immunization Program, United States, 1976--1977. Because of an increase in the number of reports of Guillian-Barre syndrome (GBS) following A/New Jersey influenza vaccination, the National Influenza Immunization Program was suspended December 16, 1976 and nationwide surveillance for GBS was begun. This surveillance uncovered a total of 1098 patients with onset of GBS from October 1, 1976, to January 31, 1977, from all 50 states, District of Columbia, and Puerto Rico. A total of 532 patients had recently received an A/New Jersey influenza vaccination prior to their onset of GBS (vaccinated cases), and 15 patients received a vaccination after their onset of GBS. Five hundred forty-three patients had not been recently vaccinated with A/New Jersey influenza vaccine and the vaccination status for 8 was unknown. Epidemiologic evidence indicated that many cases of GBS were related to vaccination. When compared to the unvaccinated population, the vaccinated population had a significantly elevated attack rate in every adult age group. The estimated attributable risk of vaccine-related GBS in the adult population was just under one case per 100,000 vaccinations. The period of increased risk was concentrated primarily within the 5-week period after vaccination, although it lasted for approximately 9 or 10 weeks."}
{"id": "PMID:463870", "title": "Five-year analysis of adenovirus 8 antibody levels in an industrial community following an outbreak of keratoconjunctivitis.", "content": "Serum hemagglutination-inhibition (HI) antibody titers were monitored for five years with samples from 79 keratoconjunctivitis patients with type 8 adenovirus (AV-8) infection. Geometric mean antibody titers declined from 1:161 at the peak of the epidemic to 1:56 after five years. Computerized analysis of the decline in geometric mean antibody titer indicates that most patients will have a serum HI titer of 1:25 by 10 years after their clinical infection. These residual antibody levels and the consistent failure to document re-infection with AV-8 suggest that recurrent episodes of keratoconjunctivitis are caused by adenovirus serotypes other than type 8.", "contents": "Five-year analysis of adenovirus 8 antibody levels in an industrial community following an outbreak of keratoconjunctivitis. Serum hemagglutination-inhibition (HI) antibody titers were monitored for five years with samples from 79 keratoconjunctivitis patients with type 8 adenovirus (AV-8) infection. Geometric mean antibody titers declined from 1:161 at the peak of the epidemic to 1:56 after five years. Computerized analysis of the decline in geometric mean antibody titer indicates that most patients will have a serum HI titer of 1:25 by 10 years after their clinical infection. These residual antibody levels and the consistent failure to document re-infection with AV-8 suggest that recurrent episodes of keratoconjunctivitis are caused by adenovirus serotypes other than type 8."}
{"id": "PMID:463871", "title": "Acquaintance networks among leukemia and lymphoma patients.", "content": "A case-control study of clustering through acquaintanceship among lymphoma and leukemia patients was conducted for the years 1967 through 1972 in Orleans County, New York. Twenty lymphoma and 17 leukemia cases met criteria for inclusion in the study. Data on acquaintanceship linkage were gathered from the source cases and controls, and from their acquaintances yielding a data base of 13,409 unique individuals linked by acquaintance. Three different analyses were carried out: a statistical analysis of linkage via intermediaries of case pairs in comparison with control pairs; a computer simulation of disease transmission from selected source cases to selected targets and controls based on the acquaintance data; and a secondary attack rate type analysis. The first two types of analysis yielded statistically significant case-control differences at the .05 level. The third method also yielded a positive result but was not subject to quantitative hypothesis testing. An additional conclusion is that these epidemiologic methods for disease with long induction periods merit further study.", "contents": "Acquaintance networks among leukemia and lymphoma patients. A case-control study of clustering through acquaintanceship among lymphoma and leukemia patients was conducted for the years 1967 through 1972 in Orleans County, New York. Twenty lymphoma and 17 leukemia cases met criteria for inclusion in the study. Data on acquaintanceship linkage were gathered from the source cases and controls, and from their acquaintances yielding a data base of 13,409 unique individuals linked by acquaintance. Three different analyses were carried out: a statistical analysis of linkage via intermediaries of case pairs in comparison with control pairs; a computer simulation of disease transmission from selected source cases to selected targets and controls based on the acquaintance data; and a secondary attack rate type analysis. The first two types of analysis yielded statistically significant case-control differences at the .05 level. The third method also yielded a positive result but was not subject to quantitative hypothesis testing. An additional conclusion is that these epidemiologic methods for disease with long induction periods merit further study."}
{"id": "PMID:463872", "title": "Gross differences observed in the placentas of smokers and nonsmokers.", "content": "Gross placental characteristics of 7651 smokers and nonsmokers are compared in light of the previous finding that placentas of smokers are heavier for the weight of their fetuses than are those of nonsmokers. Subchorionic fibrin deposits and placental calcification are more prevalent in the placentas of smokers than in those of nonsmokers. While mean placental weights and placental weight distributions for light and heavy smokers combined do not differ from those of nonsmokers, other placental measurements do differ. Smokers have thinner, rounder placentas than nonsmokers and the distance from the edge of rupture of the membranes to the placental margin is reduced among smokers. These findings are discussed in relation to placental changes observed at high altitudes and complications of pregnancy that differ for smokers and nonsmokers.", "contents": "Gross differences observed in the placentas of smokers and nonsmokers. Gross placental characteristics of 7651 smokers and nonsmokers are compared in light of the previous finding that placentas of smokers are heavier for the weight of their fetuses than are those of nonsmokers. Subchorionic fibrin deposits and placental calcification are more prevalent in the placentas of smokers than in those of nonsmokers. While mean placental weights and placental weight distributions for light and heavy smokers combined do not differ from those of nonsmokers, other placental measurements do differ. Smokers have thinner, rounder placentas than nonsmokers and the distance from the edge of rupture of the membranes to the placental margin is reduced among smokers. These findings are discussed in relation to placental changes observed at high altitudes and complications of pregnancy that differ for smokers and nonsmokers."}
{"id": "PMID:463873", "title": "Increased risk of thrombosis due to oral contraceptives: a further report.", "content": "In a previously reported case-control study of the relationship between oral contraceptives and thromboembolism, there were 461 cases and 1302 controls, individually matched on age, race, marital status, hospital, and date of admission. Initially, the control patients had not been matched with the cases for the presence or absence of six factors thought to predispose to or precipitate thromboembolic disease. The present paper reports the effects of taking into consideration these factors in the controls. Two methods of analysis (matched set, and logistic regression) gave closely similar results. Where the case series consisted of idiopathic cases, the revised estimate of the relative risk was reduced from 7.2 to 4.7 by these procedures; for predisposed cases, it was increased from 1.2 to 2.2. The explanation suggested in the previous report for the failure to find an increased risk for cases with predisposition receives support from these findings. Variation in the relative risk was examined for four separate diagnostic categories: venous thrombosis alone, pulmonary embolism alone, venous thrombosis and pulmonary embolism together, and myocardial infarction. The relative risk estimates were greater than unity for each thrombosis category for both predisposed and non-predisposed cases. The relative risk was not found to vary significantly according to age or smoking status.", "contents": "Increased risk of thrombosis due to oral contraceptives: a further report. In a previously reported case-control study of the relationship between oral contraceptives and thromboembolism, there were 461 cases and 1302 controls, individually matched on age, race, marital status, hospital, and date of admission. Initially, the control patients had not been matched with the cases for the presence or absence of six factors thought to predispose to or precipitate thromboembolic disease. The present paper reports the effects of taking into consideration these factors in the controls. Two methods of analysis (matched set, and logistic regression) gave closely similar results. Where the case series consisted of idiopathic cases, the revised estimate of the relative risk was reduced from 7.2 to 4.7 by these procedures; for predisposed cases, it was increased from 1.2 to 2.2. The explanation suggested in the previous report for the failure to find an increased risk for cases with predisposition receives support from these findings. Variation in the relative risk was examined for four separate diagnostic categories: venous thrombosis alone, pulmonary embolism alone, venous thrombosis and pulmonary embolism together, and myocardial infarction. The relative risk estimates were greater than unity for each thrombosis category for both predisposed and non-predisposed cases. The relative risk was not found to vary significantly according to age or smoking status."}
{"id": "PMID:463874", "title": "Drug utilization and reported adverse reactions in hospitalized children.", "content": "An intensive drug surveillance program has been developed to study the clinical effects of drugs in hospitalized children. This program collects information on drug exposures and the occurrence of adverse clinical events. The 1669 children monitored to date received an average of 7.6 drugs during an average hospital stay of 8.4 days. A group of specified adverse clinical events, whether or not drug attributed, occurred in 45.7% of the patients; drug-attributed events (adverse drug reactions) occurred in 16.8%. Both drug use and reported adverse reactions tended to increase with age, except that newborns received many drugs but had the lowest reported adverse reaction rates. Newborns, however, had the highest rate of adverse events not attributed to drugs, suggesting that perhaps some of these latter events include presently unrecognized adverse drug reactions.", "contents": "Drug utilization and reported adverse reactions in hospitalized children. An intensive drug surveillance program has been developed to study the clinical effects of drugs in hospitalized children. This program collects information on drug exposures and the occurrence of adverse clinical events. The 1669 children monitored to date received an average of 7.6 drugs during an average hospital stay of 8.4 days. A group of specified adverse clinical events, whether or not drug attributed, occurred in 45.7% of the patients; drug-attributed events (adverse drug reactions) occurred in 16.8%. Both drug use and reported adverse reactions tended to increase with age, except that newborns received many drugs but had the lowest reported adverse reaction rates. Newborns, however, had the highest rate of adverse events not attributed to drugs, suggesting that perhaps some of these latter events include presently unrecognized adverse drug reactions."}
{"id": "PMID:463875", "title": "Assessment of the association between habitual salt intake and high blood pressure: methodological problems.", "content": "Despite the finding in cross-cultural comparisons that habitual sodium intake correlates with levels of blood pressure, similar studies from within population groups have yielded inconsistent results. The data presented in this report indicate that in industrialized societies the high degree of intra-individual variability of sodium intake, compared to much smaller inter-individual differences, may obscure potential biological correlations. A quantitative statistical method is presented to assess and minimize the effect of the large intra-individual variation in daily urinary sodium excretion.", "contents": "Assessment of the association between habitual salt intake and high blood pressure: methodological problems. Despite the finding in cross-cultural comparisons that habitual sodium intake correlates with levels of blood pressure, similar studies from within population groups have yielded inconsistent results. The data presented in this report indicate that in industrialized societies the high degree of intra-individual variability of sodium intake, compared to much smaller inter-individual differences, may obscure potential biological correlations. A quantitative statistical method is presented to assess and minimize the effect of the large intra-individual variation in daily urinary sodium excretion."}
{"id": "PMID:463876", "title": "Evidence for a hybrid hexosaminidase isoenzyme in heterozygotes for Sandhoff disease.", "content": "Patients with Sandhoff disease have less than 5% of normal levels of serum or tissue hexosaminidase activity. They are thought to have a defect in the structural gene for the beta chain of hexosaminidase (HEX). Heterozygotes for Sandhoff disease have approximately 50% of the total serum HEX activity of normals and more than 75% of the HEX is heat-labile. In normals, only 55%--65% of serum HEX is heat-labile. Serum HEX separates into three forms on DEAE cellulose chromatography: HEX A, a tetramer of 2 alpha and 2 beta chains, and HEX I and B composed solely of beta chains. The DEAE chromatograms from normals and Sandhoff heterozygotes did not differ in the relative distribution of HEX activity between peaks. In normals, the HEX A peak was heat-labile (60 degrees C for 9 min), but HEX I and B were heat-stable. In Sandhoff heterozygotes, however, HEX I and B were only 50%--53% heat-stable. This suggests the heterozygotes synthesized a hybrid enzyme containing both mutant and wild-type beta chains for HEX. The mutant beta chain renders the isoenzyme less stable to heating.", "contents": "Evidence for a hybrid hexosaminidase isoenzyme in heterozygotes for Sandhoff disease. Patients with Sandhoff disease have less than 5% of normal levels of serum or tissue hexosaminidase activity. They are thought to have a defect in the structural gene for the beta chain of hexosaminidase (HEX). Heterozygotes for Sandhoff disease have approximately 50% of the total serum HEX activity of normals and more than 75% of the HEX is heat-labile. In normals, only 55%--65% of serum HEX is heat-labile. Serum HEX separates into three forms on DEAE cellulose chromatography: HEX A, a tetramer of 2 alpha and 2 beta chains, and HEX I and B composed solely of beta chains. The DEAE chromatograms from normals and Sandhoff heterozygotes did not differ in the relative distribution of HEX activity between peaks. In normals, the HEX A peak was heat-labile (60 degrees C for 9 min), but HEX I and B were heat-stable. In Sandhoff heterozygotes, however, HEX I and B were only 50%--53% heat-stable. This suggests the heterozygotes synthesized a hybrid enzyme containing both mutant and wild-type beta chains for HEX. The mutant beta chain renders the isoenzyme less stable to heating."}
{"id": "PMID:463877", "title": "Familial hyperlysinemia: enzyme studies, diagnostic methods, comments on terminology.", "content": "Enzyme assays of skin fibroblasts from five children with familial hyperlysinemia from unrelated families are added to the previous report of three children from two unrelated families. In all instances there was a deficiency in lysine-ketoglutarate reductase, saccharopine dehydrogenase, and saccharopine oxidoreductase activities. To complete the studies on the enzymes associated with familial hyperlysinemia, saccharopine oxidoreductase was partially purified from human liver and characterized. The activity did not separate from that of lysine-ketoglutarate reductase or saccharopine dehydrogenase. A simple screening test for familial hyperlysinemia is described based on the evolution of 14CO2 from lysine-14C by skin fibroblasts. The test differentiated, without overlap, seven patients with familial hyperlysinemia from control subjects. The relation of the two genetic entities involving lysine degradation, familial hyperlysinemia and saccharopinuria, is discussed. It is suggested that familial hyperlysinemia, type I, be applied to patients with major defects in lysine-ketoglutarate reductase and saccharopine dehydrogenase, and that familial hyperlysinemia, type II, to be used to designate patients in whom significant amounts of lysine-ketoglutarate reductase are retained. The nomenclature would be consistent with that of an analogous disease, orotic aciduria.", "contents": "Familial hyperlysinemia: enzyme studies, diagnostic methods, comments on terminology. Enzyme assays of skin fibroblasts from five children with familial hyperlysinemia from unrelated families are added to the previous report of three children from two unrelated families. In all instances there was a deficiency in lysine-ketoglutarate reductase, saccharopine dehydrogenase, and saccharopine oxidoreductase activities. To complete the studies on the enzymes associated with familial hyperlysinemia, saccharopine oxidoreductase was partially purified from human liver and characterized. The activity did not separate from that of lysine-ketoglutarate reductase or saccharopine dehydrogenase. A simple screening test for familial hyperlysinemia is described based on the evolution of 14CO2 from lysine-14C by skin fibroblasts. The test differentiated, without overlap, seven patients with familial hyperlysinemia from control subjects. The relation of the two genetic entities involving lysine degradation, familial hyperlysinemia and saccharopinuria, is discussed. It is suggested that familial hyperlysinemia, type I, be applied to patients with major defects in lysine-ketoglutarate reductase and saccharopine dehydrogenase, and that familial hyperlysinemia, type II, to be used to designate patients in whom significant amounts of lysine-ketoglutarate reductase are retained. The nomenclature would be consistent with that of an analogous disease, orotic aciduria."}
{"id": "PMID:463879", "title": "Triple-spot proteins in two-dimensional gel electrophoresis.", "content": "A triple-spot pattern of polypeptides occurring in two-dimensional gel electrophoresis of proteins is described. The presence of a mutant protein, Pc 1 Duarte, which results in a splitting of all three polypeptides, is evidence that they are produced by the same gene. This pattern is seen in about 1% of the proteins from a variety of sources. Typically, about 50% of the protein occurs as a single major spot, the remainder occurring as two polypeptides with an additional negative charge and slightly different molecular weight. The reproducibility of this pattern implies a functional significance which is presently unknown. The implication of this configuration for patterns seen by one-dimensional gel electrophoresis is discussed.", "contents": "Triple-spot proteins in two-dimensional gel electrophoresis. A triple-spot pattern of polypeptides occurring in two-dimensional gel electrophoresis of proteins is described. The presence of a mutant protein, Pc 1 Duarte, which results in a splitting of all three polypeptides, is evidence that they are produced by the same gene. This pattern is seen in about 1% of the proteins from a variety of sources. Typically, about 50% of the protein occurs as a single major spot, the remainder occurring as two polypeptides with an additional negative charge and slightly different molecular weight. The reproducibility of this pattern implies a functional significance which is presently unknown. The implication of this configuration for patterns seen by one-dimensional gel electrophoresis is discussed."}
{"id": "PMID:463878", "title": "Hemolytic anemia due to pyruvate kinase deficiency: characterization of the enzymatic activity from eight patients.", "content": "We have studied the red cell pyruvate kinase (PK) variants from eight patients representing five families with pyruvate kinase deficiency-associated hemolytic anemia. The kinetic properties, electrophoretic mobilities, and immunological reactivity with anti-normal red cell pyruvate kinase were determined. The patients differ in the severity of their clinical condition and in the molecular properties of their red cell pyruvate kinase variants. The most seriously affected patient (PK Beaverton) has no electrophoretically demonstrable red cell isozymes. The activity present is due to the M2 isozyme, however red cell isozyme can be detected immunologically. PK Molalla and PK Lake Oswego are thermolabile variants with normal kinetic parameters. PK Molalla, in addition, has altered electrophoretic mobility. PK Multnomah and PK Milwaukie have decreased affinity for the substrate phosphoenolpyruvate, and PK Multnomah also has altered electrophoretic mobility. PK Coos Bay shows electrophoretic variation and a slightly decreased affinity for phosphoenolpyruvate consistent with an increased modulating effect of fructose-1,6-diphosphate.", "contents": "Hemolytic anemia due to pyruvate kinase deficiency: characterization of the enzymatic activity from eight patients. We have studied the red cell pyruvate kinase (PK) variants from eight patients representing five families with pyruvate kinase deficiency-associated hemolytic anemia. The kinetic properties, electrophoretic mobilities, and immunological reactivity with anti-normal red cell pyruvate kinase were determined. The patients differ in the severity of their clinical condition and in the molecular properties of their red cell pyruvate kinase variants. The most seriously affected patient (PK Beaverton) has no electrophoretically demonstrable red cell isozymes. The activity present is due to the M2 isozyme, however red cell isozyme can be detected immunologically. PK Molalla and PK Lake Oswego are thermolabile variants with normal kinetic parameters. PK Molalla, in addition, has altered electrophoretic mobility. PK Multnomah and PK Milwaukie have decreased affinity for the substrate phosphoenolpyruvate, and PK Multnomah also has altered electrophoretic mobility. PK Coos Bay shows electrophoretic variation and a slightly decreased affinity for phosphoenolpyruvate consistent with an increased modulating effect of fructose-1,6-diphosphate."}
{"id": "PMID:463880", "title": "Maternal effects on fingertip dermatoglyphics.", "content": "Significantly larger variation between sibships within families of male MZ twins than between sibships within families of female MZ twins, indicative of maternal influences, was found for 10 of 41 dermatoglyphic fingertip variables. Of these, five were thumb-related with the effect primarily on the thumb radial and ridge count (larger of radial and ulnar count). These same variables were previously found to have unequal variances in MZ twins of known placental type, and the results indicate maternal influences in singletons as well as twins for these variables. Although the total ridge count (TRC), previously shown to differ in MZ twins of known placental type (paralleling the thumb radial and ridge counts) did not reach significance, the trend indicated that the observed thumb changes may be reflected in the TRC as well. Little finger pattern type and ulnar counts also showed less variability in families of female MZ twins, but the interpretation is complicated by the concomitant differences in mean squares within-sibships for these little finger variables.", "contents": "Maternal effects on fingertip dermatoglyphics. Significantly larger variation between sibships within families of male MZ twins than between sibships within families of female MZ twins, indicative of maternal influences, was found for 10 of 41 dermatoglyphic fingertip variables. Of these, five were thumb-related with the effect primarily on the thumb radial and ridge count (larger of radial and ulnar count). These same variables were previously found to have unequal variances in MZ twins of known placental type, and the results indicate maternal influences in singletons as well as twins for these variables. Although the total ridge count (TRC), previously shown to differ in MZ twins of known placental type (paralleling the thumb radial and ridge counts) did not reach significance, the trend indicated that the observed thumb changes may be reflected in the TRC as well. Little finger pattern type and ulnar counts also showed less variability in families of female MZ twins, but the interpretation is complicated by the concomitant differences in mean squares within-sibships for these little finger variables."}
{"id": "PMID:463892", "title": "Drug therapy for Reye's syndrome.", "content": "The pathogenesis, biochemical defects, pathophysiology, clinical and laboratory symptoms, and treatment of Reye's syndrome are reviewed. The etiology of the syndrome is unknown, but it appears to be a viral interference with basic biochemical processes. Increased intracranial pressure is one of the most lethal aspects of the disease; cerebral edema often ensues. Physiologic and pharmacologic methods of treating cerebral edema, including the use of osmotically active agents (mannitol, urea and glycerol), corti-costeroids and potent diuretics are reviewed. Correction of the biochemical abnormalities with enzyme and nutritional factor replacement is discussed. Intensive supportive care, including maintenance of fluid and electrolytes, reduction of intracranial pressure and elective endotracheal intubation, is the mainstay of treatment.", "contents": "Drug therapy for Reye's syndrome. The pathogenesis, biochemical defects, pathophysiology, clinical and laboratory symptoms, and treatment of Reye's syndrome are reviewed. The etiology of the syndrome is unknown, but it appears to be a viral interference with basic biochemical processes. Increased intracranial pressure is one of the most lethal aspects of the disease; cerebral edema often ensues. Physiologic and pharmacologic methods of treating cerebral edema, including the use of osmotically active agents (mannitol, urea and glycerol), corti-costeroids and potent diuretics are reviewed. Correction of the biochemical abnormalities with enzyme and nutritional factor replacement is discussed. Intensive supportive care, including maintenance of fluid and electrolytes, reduction of intracranial pressure and elective endotracheal intubation, is the mainstay of treatment."}
{"id": "PMID:463893", "title": "Lidocaine infusions: effect of duration and method of discontinuation on recurrence of arrhythmias and pharmacokinetic variables.", "content": "The effect of abrupt versus gradual discontinuation of lidocaine hydrochloride infusion on the incidence of recurrent ventricular arrhythmia and on lidocaine pharmacokinetics was studied. Twenty-nine patients with documented myocardial infarction receiving no other antiarrhythmic drugs and having no evidence of congestive heart failure, liver disease or renal failure were randomly assigned to one or two groups: Group 1--lidocaine infusion was abruptly discontinued after a course of therapy, and Group 2--lidocaine infusion was tapered over a three-hour period. Lidocaine blood levels were measured at the abrupt discontinuation of an infusion or the initiation of tapering and three hours later. Cardiac rhythms were monitored until patients were transferred from the coronary care unit. Three of 18 patients in Group I and two of 11 patients in Group 2 had recurrent ventricular arrhythmias at least 12 hours after idocaine infusion was stopped. The duration of infusion did not correlate with the recurrence of ventricular arrhythmia. In patients infused with lidocaine for 36 hours or less, apparent body clearance was significantly faster than that in patients infused with lidocaine for longer than 36 hours (p less than 0.01). There was no difference in the incidence of recurrent ventricular arrhythmias when comparing abrupt cessation of lidocaine with three-hour tapering. Lidocaine infusion rates may have to be decreased after 36 hours to avoid accumulation of the drug.", "contents": "Lidocaine infusions: effect of duration and method of discontinuation on recurrence of arrhythmias and pharmacokinetic variables. The effect of abrupt versus gradual discontinuation of lidocaine hydrochloride infusion on the incidence of recurrent ventricular arrhythmia and on lidocaine pharmacokinetics was studied. Twenty-nine patients with documented myocardial infarction receiving no other antiarrhythmic drugs and having no evidence of congestive heart failure, liver disease or renal failure were randomly assigned to one or two groups: Group 1--lidocaine infusion was abruptly discontinued after a course of therapy, and Group 2--lidocaine infusion was tapered over a three-hour period. Lidocaine blood levels were measured at the abrupt discontinuation of an infusion or the initiation of tapering and three hours later. Cardiac rhythms were monitored until patients were transferred from the coronary care unit. Three of 18 patients in Group I and two of 11 patients in Group 2 had recurrent ventricular arrhythmias at least 12 hours after idocaine infusion was stopped. The duration of infusion did not correlate with the recurrence of ventricular arrhythmia. In patients infused with lidocaine for 36 hours or less, apparent body clearance was significantly faster than that in patients infused with lidocaine for longer than 36 hours (p less than 0.01). There was no difference in the incidence of recurrent ventricular arrhythmias when comparing abrupt cessation of lidocaine with three-hour tapering. Lidocaine infusion rates may have to be decreased after 36 hours to avoid accumulation of the drug."}
{"id": "PMID:463894", "title": "Improved methods for estimating initial heparin infusion rates.", "content": "Two methods of calculating heparin infusion rates for patients with venous thrombotic disease were compared; one method was based on a one-compartment pharmacokinetic model, the other on patient weight. Sixty-eight patients with presumed thromboembolic disease were started on continuous i.v. heparin sodium (porcine) using an infusion pump. Patients were divided into two groups--the infusion rate of Group I was based on patient weight (77 units/kg/4 hrs) and the infusion rate of Group 2 was determined by a pharmacokinetic equation based on a one-compartment heparin model. Heparin effect was measured by an activated partial thromboplastin time (APTT). The initial heparin infusion rate for Group 1 (4,784 +/- 672 units/4 hrs) was significantly greater (p less than 0.039, two-sample t-test) than that for Group 2 (4,413 +/- 779 units/4 hrs), but the variances of the rates were not significantly different (p = 0.40, ratio of variance F-test). Both methods for estimating initial heparin infusion rates gave mean APTT values in the center of the therapeutic range, but the variance in the APTTs of Group 2 patients was significantly smaller (p = 0.004) than that of Group 1. The pharmacokinetic model was more precise and reliable. This model should be valuable for insuring heparin's therapeutic effect without exposing patients to the potential risk of hemorrhage.", "contents": "Improved methods for estimating initial heparin infusion rates. Two methods of calculating heparin infusion rates for patients with venous thrombotic disease were compared; one method was based on a one-compartment pharmacokinetic model, the other on patient weight. Sixty-eight patients with presumed thromboembolic disease were started on continuous i.v. heparin sodium (porcine) using an infusion pump. Patients were divided into two groups--the infusion rate of Group I was based on patient weight (77 units/kg/4 hrs) and the infusion rate of Group 2 was determined by a pharmacokinetic equation based on a one-compartment heparin model. Heparin effect was measured by an activated partial thromboplastin time (APTT). The initial heparin infusion rate for Group 1 (4,784 +/- 672 units/4 hrs) was significantly greater (p less than 0.039, two-sample t-test) than that for Group 2 (4,413 +/- 779 units/4 hrs), but the variances of the rates were not significantly different (p = 0.40, ratio of variance F-test). Both methods for estimating initial heparin infusion rates gave mean APTT values in the center of the therapeutic range, but the variance in the APTTs of Group 2 patients was significantly smaller (p = 0.004) than that of Group 1. The pharmacokinetic model was more precise and reliable. This model should be valuable for insuring heparin's therapeutic effect without exposing patients to the potential risk of hemorrhage."}
{"id": "PMID:463895", "title": "Pharmacist involvement in a deferoxamine education program for patients with Cooley's anemia.", "content": "A program to train patients with Cooley's anemia to self-administer deferoxamine via an intermittent infusion pump is described. A pharmacist provides the patient and his family with indepth information on the disease and on deferoxamine dosage, preparation, subcutaneous administration, stability, storage, side effects and precautions. Patients are trained by the pharmacist in aseptic technique and in use and maintenance of the intermittent infusion pump. The patient education program allows patients with Cooley's anemia to receive their daily deferoxamine therapy without the neef for frequent hospitalization.", "contents": "Pharmacist involvement in a deferoxamine education program for patients with Cooley's anemia. A program to train patients with Cooley's anemia to self-administer deferoxamine via an intermittent infusion pump is described. A pharmacist provides the patient and his family with indepth information on the disease and on deferoxamine dosage, preparation, subcutaneous administration, stability, storage, side effects and precautions. Patients are trained by the pharmacist in aseptic technique and in use and maintenance of the intermittent infusion pump. The patient education program allows patients with Cooley's anemia to receive their daily deferoxamine therapy without the neef for frequent hospitalization."}
{"id": "PMID:463896", "title": "Pharmacy internship: patient care experience through nursing service.", "content": "A pharmacy internship that offers graduated levels of hospital experience, with an emphasis on nursing assistant and medication administration programs, is described. Students work full time in the summer and part time during the academic year in the three-year program. The progression of duties is as follows: nursing assistant; inpatient drug distribution and control; sterile and nonsterile product production; patient education, monitoring and medication history-taking activities; drug information services; and medication administration. Interns are selected by the pharmacy department and trained and supervised by the nursing staff in nursing assistant and medication aide roles. The program provides the pharmacy department with a highly trained corps of interns who understand and appreciate the full range of systems required to deliver patient care and provides interns with patient-oriented work experiences valuable in their future roles as pharmacists.", "contents": "Pharmacy internship: patient care experience through nursing service. A pharmacy internship that offers graduated levels of hospital experience, with an emphasis on nursing assistant and medication administration programs, is described. Students work full time in the summer and part time during the academic year in the three-year program. The progression of duties is as follows: nursing assistant; inpatient drug distribution and control; sterile and nonsterile product production; patient education, monitoring and medication history-taking activities; drug information services; and medication administration. Interns are selected by the pharmacy department and trained and supervised by the nursing staff in nursing assistant and medication aide roles. The program provides the pharmacy department with a highly trained corps of interns who understand and appreciate the full range of systems required to deliver patient care and provides interns with patient-oriented work experiences valuable in their future roles as pharmacists."}
{"id": "PMID:463897", "title": "Method for evaluating drug information systems.", "content": "A method for evaluating drug information systems is described. The method measures both qualitative and quantitative performance. For the categories, \"Basic Therapeutic Information\", and \"Commercial and Investigational Product Information,\" specific data that should be present are identified; evaluated systems are searched for these data related to a standard sample of 122 drugs. Systems are also searched for the presence of any information relating to these drugs in the categories, \"Applied Clinical Research Information\" and \"Nonclinical Laboratory Research Information\". In addition, systems are searched for the presence of 215 drug-drug interactions and 244 drug-laboratory test interferences. Using relative weights that have been assigned to the content statistics, an aggregate measure of system's content is determined. The method also measures annual cost of operating a system. The application of the method to two drug information systems is discussed briefly.", "contents": "Method for evaluating drug information systems. A method for evaluating drug information systems is described. The method measures both qualitative and quantitative performance. For the categories, \"Basic Therapeutic Information\", and \"Commercial and Investigational Product Information,\" specific data that should be present are identified; evaluated systems are searched for these data related to a standard sample of 122 drugs. Systems are also searched for the presence of any information relating to these drugs in the categories, \"Applied Clinical Research Information\" and \"Nonclinical Laboratory Research Information\". In addition, systems are searched for the presence of 215 drug-drug interactions and 244 drug-laboratory test interferences. Using relative weights that have been assigned to the content statistics, an aggregate measure of system's content is determined. The method also measures annual cost of operating a system. The application of the method to two drug information systems is discussed briefly."}
{"id": "PMID:463898", "title": "Sucrose as a sweetener for activated charcoal.", "content": "The efficacy of sucrose as a flavor for activated charcoal was studied. In vitro adsorption of sucrose (in Simulated Gastric Fluid, USP, without pepsin) to activated charcoal, and of a 1-g/liter sodium salicylate solution to a 1:1 mixture of sucrose and activated charcoal and to plain activated charcoal, was measured spectrophotometrically. In vitro adsorption of sucroses to activated charcoal was minimal. Sucrose reduced in vitro adsorption of sodium salicylate to activated charcoal by only small amounts. For example, at a ratio of 4 g activated charcoal to 1 g sodium salicylate, sucrose reduced salicylate adsorption to activated charcoal from 99% to 95%. A 1:1 sucrose-activated charcoal preparation provides sufficient flavor without substantial loss of adsorbance.", "contents": "Sucrose as a sweetener for activated charcoal. The efficacy of sucrose as a flavor for activated charcoal was studied. In vitro adsorption of sucrose (in Simulated Gastric Fluid, USP, without pepsin) to activated charcoal, and of a 1-g/liter sodium salicylate solution to a 1:1 mixture of sucrose and activated charcoal and to plain activated charcoal, was measured spectrophotometrically. In vitro adsorption of sucroses to activated charcoal was minimal. Sucrose reduced in vitro adsorption of sodium salicylate to activated charcoal by only small amounts. For example, at a ratio of 4 g activated charcoal to 1 g sodium salicylate, sucrose reduced salicylate adsorption to activated charcoal from 99% to 95%. A 1:1 sucrose-activated charcoal preparation provides sufficient flavor without substantial loss of adsorbance."}
{"id": "PMID:463899", "title": "Therapeutic problem solving: a systematic approach.", "content": "A systematic approach to therapeutic problem solving is discussed. Therapeutic problem solving includes defining the problem, assessing possible solutions to the problem and choosing the best solution (therapy). The thought processes specific to drug therapy include: (1) choice of agent, (2) evaluation of benefit versus risk ratios, and (3) determination of dosage. Once treatment has been initiated, the problem-solving process shifts to reassessing the patient, monitoring and readjusting therapy, and anticipating new problems related to therapy. An algorithm is provided to demonstrate a thought sequence for solving therapeutic problems. To assure that no important consideration has been neglected, it is useful to develop a pattern of thought that may be applied to each therapeutic situation.", "contents": "Therapeutic problem solving: a systematic approach. A systematic approach to therapeutic problem solving is discussed. Therapeutic problem solving includes defining the problem, assessing possible solutions to the problem and choosing the best solution (therapy). The thought processes specific to drug therapy include: (1) choice of agent, (2) evaluation of benefit versus risk ratios, and (3) determination of dosage. Once treatment has been initiated, the problem-solving process shifts to reassessing the patient, monitoring and readjusting therapy, and anticipating new problems related to therapy. An algorithm is provided to demonstrate a thought sequence for solving therapeutic problems. To assure that no important consideration has been neglected, it is useful to develop a pattern of thought that may be applied to each therapeutic situation."}
{"id": "PMID:463900", "title": "Using pharmacokinetics in drug therapy. IV: Consideration in applying pharmacokinetic and pharmacodynamic estimates to antihypertensive dosage regimens.", "content": "A theory explaining the pharmacokinetics of drugs with direct and rapidly reversible pharmacologic effects is reviewed, and the application of this theory to individualizing dosage regimens for antihypertensive drugs is discussed. In a few studies, investigators have correlated, under controlled conditions and in a small number of patients, the pharmacokinetic variables needed to individualize minoxidil and clonidine dosage regimens with clinical responses (mean arterial blood pressure). The potential clinical application of these correlations to other antihypertensive drugs was studied by retrospectively analyzing diastolic and mean arterial blood pressure data reported in 26 published studies of seven antihypertensive drugs. The wide interpatient variation recorded in the dose-response plots derived from the published data yielded pharmacodynamic values of little reliability or predictive value in individualizing antihypertensive dosage regimens. Use of retrospective data analysis for obtaining the necessary pharmacokinetic values to individualize antihypertensive dosage regimens is discouraged.", "contents": "Using pharmacokinetics in drug therapy. IV: Consideration in applying pharmacokinetic and pharmacodynamic estimates to antihypertensive dosage regimens. A theory explaining the pharmacokinetics of drugs with direct and rapidly reversible pharmacologic effects is reviewed, and the application of this theory to individualizing dosage regimens for antihypertensive drugs is discussed. In a few studies, investigators have correlated, under controlled conditions and in a small number of patients, the pharmacokinetic variables needed to individualize minoxidil and clonidine dosage regimens with clinical responses (mean arterial blood pressure). The potential clinical application of these correlations to other antihypertensive drugs was studied by retrospectively analyzing diastolic and mean arterial blood pressure data reported in 26 published studies of seven antihypertensive drugs. The wide interpatient variation recorded in the dose-response plots derived from the published data yielded pharmacodynamic values of little reliability or predictive value in individualizing antihypertensive dosage regimens. Use of retrospective data analysis for obtaining the necessary pharmacokinetic values to individualize antihypertensive dosage regimens is discouraged."}
{"id": "PMID:463901", "title": "Relapsing polychondritis in a Latin American man.", "content": "The first known reported case of relapsing polychondritis in a Latin American man is presented. The 35-year-old man, demonstrating auricular chondritis, arthritis, nasal cartilage involvement, episcleritis and respiratory tract chondritis, was admitted to the hospital and treated with 60 mg of prednisone daily, prednisolone acetate 1% ophthalmic drops every waking hour and homatropine hydrobromide 5% ophthalmic drops twice daily. After discharge from the hospital, he received 30 to 60 mg of prednisone daily and prednisolone acetate 1% ophthalmic drops twic daily for about 18 months. A permanent trachestomy was placed, azathioprine, 150 mg daily, was given and prednisone dosage was tapered to 25 mg daily when the patient subsequently was hospitalized for colapsed airway and Cushing's syndrome. The classic symptoms, pathogenesis and treatment of the disease are reviewed. Systemic corticosteroids are the drugs of choice in relapsing polychondritis, with immunosuppressive drugs, azathioprine in particular, being used as adjuvants to lower steroid dosage requirements and to achieve greater control of symptoms in patients with the severe progressive disease. Treatment of the disease with dapsone alone is promising and may offer an alternative to high-dose steroids and immunosuppressive therapy.", "contents": "Relapsing polychondritis in a Latin American man. The first known reported case of relapsing polychondritis in a Latin American man is presented. The 35-year-old man, demonstrating auricular chondritis, arthritis, nasal cartilage involvement, episcleritis and respiratory tract chondritis, was admitted to the hospital and treated with 60 mg of prednisone daily, prednisolone acetate 1% ophthalmic drops every waking hour and homatropine hydrobromide 5% ophthalmic drops twice daily. After discharge from the hospital, he received 30 to 60 mg of prednisone daily and prednisolone acetate 1% ophthalmic drops twic daily for about 18 months. A permanent trachestomy was placed, azathioprine, 150 mg daily, was given and prednisone dosage was tapered to 25 mg daily when the patient subsequently was hospitalized for colapsed airway and Cushing's syndrome. The classic symptoms, pathogenesis and treatment of the disease are reviewed. Systemic corticosteroids are the drugs of choice in relapsing polychondritis, with immunosuppressive drugs, azathioprine in particular, being used as adjuvants to lower steroid dosage requirements and to achieve greater control of symptoms in patients with the severe progressive disease. Treatment of the disease with dapsone alone is promising and may offer an alternative to high-dose steroids and immunosuppressive therapy."}
{"id": "PMID:463904", "title": "Lepromatous leprosy masquerading as disseminated tuberculosis.", "content": "A patients with disseminated leprosy is described. A 57 year old man from Cuba presented with fever and pancytopenia. Bone marrow aspirate showed numerous acid-fast bacilli and a liver biopsy specimen contained multiple granulomas. The patient was considered to have tuberculosis and was treated with isoniazid and rifampin, with initial clinical improvement, only to have the fever recur and to show deterioration in hematologic and hepatic function. Failure to grow M. tuberculosis suggested a diagnosis of leprosy which was proved by skin biopsy. How lepromatous leprosy can masquerade as disseminated tuberculosis is discussed.", "contents": "Lepromatous leprosy masquerading as disseminated tuberculosis. A patients with disseminated leprosy is described. A 57 year old man from Cuba presented with fever and pancytopenia. Bone marrow aspirate showed numerous acid-fast bacilli and a liver biopsy specimen contained multiple granulomas. The patient was considered to have tuberculosis and was treated with isoniazid and rifampin, with initial clinical improvement, only to have the fever recur and to show deterioration in hematologic and hepatic function. Failure to grow M. tuberculosis suggested a diagnosis of leprosy which was proved by skin biopsy. How lepromatous leprosy can masquerade as disseminated tuberculosis is discussed."}
{"id": "PMID:463905", "title": "Nitrofurantoin: another cause of drug-induced chronic active hepatitis? A report of a patient with HLA-B8 antigen.", "content": "Nitrofurantoin, an agent used extensively in chronic urinary tract infections, is rarely incriminated as a hepatotoxin. In this 27 year old women chronic active hepatitis first developed during exposures to nitrofurantoin, and she later suffered exacerbations. Earlier reported cases are reviewed which describe a spectrum of hepatotoxic reactions. This patient has HLA-B8, an antigen associated with autoimmune forms of chronic active hepatitis. It is postulated that the antigen may be associated with liver injury initiated by drugs such as nitrofurantoin.", "contents": "Nitrofurantoin: another cause of drug-induced chronic active hepatitis? A report of a patient with HLA-B8 antigen. Nitrofurantoin, an agent used extensively in chronic urinary tract infections, is rarely incriminated as a hepatotoxin. In this 27 year old women chronic active hepatitis first developed during exposures to nitrofurantoin, and she later suffered exacerbations. Earlier reported cases are reviewed which describe a spectrum of hepatotoxic reactions. This patient has HLA-B8, an antigen associated with autoimmune forms of chronic active hepatitis. It is postulated that the antigen may be associated with liver injury initiated by drugs such as nitrofurantoin."}
{"id": "PMID:463906", "title": "Morphologic, biochemical and physiologic alterations in a case of idiopathic hypoalbuminemia (analbuminemia).", "content": "A patient with idiopathic hypoalbuminemia is described. A study of albumin kinetics demonstrated slowed albumin degradation suggesting low albumin synthesis. Morphologic observation of hepatocellular alterations suggested decreased protein synthesis. The intra- and extravascular space was low with an abnormally large postural shift of intravascular fluid into the extravascular compartment. Disturbances in the concentration of plasma lipids and of several plasma proteins were detected. The effect of albumin infusion on these physiologic and biochemical abnormalities suggests that most occurred as a secondary response to the hypoalbuminemic state. Evaluation of the patient's kindred revealed no members with hypoalbuminemia.", "contents": "Morphologic, biochemical and physiologic alterations in a case of idiopathic hypoalbuminemia (analbuminemia). A patient with idiopathic hypoalbuminemia is described. A study of albumin kinetics demonstrated slowed albumin degradation suggesting low albumin synthesis. Morphologic observation of hepatocellular alterations suggested decreased protein synthesis. The intra- and extravascular space was low with an abnormally large postural shift of intravascular fluid into the extravascular compartment. Disturbances in the concentration of plasma lipids and of several plasma proteins were detected. The effect of albumin infusion on these physiologic and biochemical abnormalities suggests that most occurred as a secondary response to the hypoalbuminemic state. Evaluation of the patient's kindred revealed no members with hypoalbuminemia."}
{"id": "PMID:463907", "title": "Testicular sarcoidosis.", "content": "A 20 year old black man presented with shortness of breath, hilar adenopathy, monoarticular arthritis, polydipsia and polyuria, and bilateral testicular masses. Transbronchial biopsy revealed noncaseating granulomas consistent with sarcoidosis. Subsequent work-up demonstrated hypothalamic dysfunction, and joint fluid analysis was consistent with sarcoid arthropathy. Concern over the possibility of a testicular neoplasm led to right testicular biopsy which revealed noncaseating granulomas consistent with testicular sarcoidosis. A short course of steroid therapy resulted in clinical improvement and complete resolution of the remaining left testicular mass.", "contents": "Testicular sarcoidosis. A 20 year old black man presented with shortness of breath, hilar adenopathy, monoarticular arthritis, polydipsia and polyuria, and bilateral testicular masses. Transbronchial biopsy revealed noncaseating granulomas consistent with sarcoidosis. Subsequent work-up demonstrated hypothalamic dysfunction, and joint fluid analysis was consistent with sarcoid arthropathy. Concern over the possibility of a testicular neoplasm led to right testicular biopsy which revealed noncaseating granulomas consistent with testicular sarcoidosis. A short course of steroid therapy resulted in clinical improvement and complete resolution of the remaining left testicular mass."}
{"id": "PMID:463908", "title": "Acute myelofibrosis in progressive systemic sclerosis. Report of a case developing during long term chlorambucil therapy and discussion of pathogenetic factors.", "content": "A case of acute myelofibrosis occurring in the course of long-term chlorambucil therapy for cardiac manifestations of progressive systemic sclerosis (PSS) is reported. Although hematologic malignancies have been known to develop consequent to long-term use of alkylating agents, and bone marrow suppression is well known, acute myelofibrosis has not, to our knowledge, been reported as a complication of this therapy, nor has any specific myeloproliferative syndrome been described consequent to such therapy in a patient with PSS. As abnormal fibroblastic proliferation is central to both PSS and myelofibrosis, it may be speculated that the patient with scleroderma may be predisposed to this particular expression of hematologic dyscrasia. The roles of fibroblastic proliferation, and immunologic and vascular factors in these two illnesses are currently poorly understood.", "contents": "Acute myelofibrosis in progressive systemic sclerosis. Report of a case developing during long term chlorambucil therapy and discussion of pathogenetic factors. A case of acute myelofibrosis occurring in the course of long-term chlorambucil therapy for cardiac manifestations of progressive systemic sclerosis (PSS) is reported. Although hematologic malignancies have been known to develop consequent to long-term use of alkylating agents, and bone marrow suppression is well known, acute myelofibrosis has not, to our knowledge, been reported as a complication of this therapy, nor has any specific myeloproliferative syndrome been described consequent to such therapy in a patient with PSS. As abnormal fibroblastic proliferation is central to both PSS and myelofibrosis, it may be speculated that the patient with scleroderma may be predisposed to this particular expression of hematologic dyscrasia. The roles of fibroblastic proliferation, and immunologic and vascular factors in these two illnesses are currently poorly understood."}
{"id": "PMID:463909", "title": "Vasculitis and Sj\u00f6gren's syndrome with IgA-IgG cryoglobulinemia terminating in immunoblastic sarcoma.", "content": "Generalized lymphadenopathy and other manifestations of Sj\u00f6gren's syndrome developed in a 68 year old woman with a long history of systemic vasculitis and arthralgia. An unusual immunologic feature was hypogammaglobulinemia and immunoglobulin A (IgA) monoclonal immunoglobulinemia with mixed IgA-IgG cryoglobulin. At autopsy, the histopathologic findings were compatible with immunoblastic sarcoma. The monoclonal IgA protein, found in serum, pleural and pericardial fluids, showed rheumatoid factor activity. Immunocytes from the immunoblastic sarcoma were found to be the source of the monoclonal IgA protein.", "contents": "Vasculitis and Sj\u00f6gren's syndrome with IgA-IgG cryoglobulinemia terminating in immunoblastic sarcoma. Generalized lymphadenopathy and other manifestations of Sj\u00f6gren's syndrome developed in a 68 year old woman with a long history of systemic vasculitis and arthralgia. An unusual immunologic feature was hypogammaglobulinemia and immunoglobulin A (IgA) monoclonal immunoglobulinemia with mixed IgA-IgG cryoglobulin. At autopsy, the histopathologic findings were compatible with immunoblastic sarcoma. The monoclonal IgA protein, found in serum, pleural and pericardial fluids, showed rheumatoid factor activity. Immunocytes from the immunoblastic sarcoma were found to be the source of the monoclonal IgA protein."}
{"id": "PMID:463910", "title": "Familial recurrent rhabdomyolysis due to carnitine palmityl transferase deficiency.", "content": "Muscle carnitine palmityltransferase (CPT) activity was very low (0 to 14 per cent of controls) in two brothers with a syndrome of recurrent rhabdomyolysis and myoglobulinuria. In isolated muscle mitochondria the majority (87.5 per cent) of total measurable CPT enzyme activity could be attributed to external membrane CPT with severe deficiency of inner membrane CPT. By contrast, control mitochondria demonstrated a 1:1 distribution of external membrane CPT to inner membrane CPT. Thus, myoglobinuria may be due to a genetic defect of lipid metabolism in skeletal muscle, with inner membrane CPT deficiency presenting the same clinical features as external membrane CPT deficiency.", "contents": "Familial recurrent rhabdomyolysis due to carnitine palmityl transferase deficiency. Muscle carnitine palmityltransferase (CPT) activity was very low (0 to 14 per cent of controls) in two brothers with a syndrome of recurrent rhabdomyolysis and myoglobulinuria. In isolated muscle mitochondria the majority (87.5 per cent) of total measurable CPT enzyme activity could be attributed to external membrane CPT with severe deficiency of inner membrane CPT. By contrast, control mitochondria demonstrated a 1:1 distribution of external membrane CPT to inner membrane CPT. Thus, myoglobinuria may be due to a genetic defect of lipid metabolism in skeletal muscle, with inner membrane CPT deficiency presenting the same clinical features as external membrane CPT deficiency."}
{"id": "PMID:463912", "title": "Diagnosis and localization of pheochromocytoma. Detection by measurement of urinary norepinephrine excretion during sleep, plasma norepinephrine concentration and computerized axial tomography (CT-scan).", "content": "The feasibility of differentiating patients with pheochromocytoma from other hypertensive patients by measuring urinary excretion rates of norepinephrine during sleep, a period of physiologic suppression of norepinephrine release, was investigated. The mean excretion rates of norepinephrine in 248 normal subjects and in 109 patients with essential hypertension were 1.03 +/- 0.03 and 1.12 +/- 0.06 (SEM) micrograms/hour, respectively, whereas the lowest excretion rate among the six patients with pheochromocytoma was about seven times higher. Plasma norepinephrine concentration in patients with pheochromocytoma was also consistently above the range observed in both normotensive and hypertensive subjects. CT scan correctly identified the same tumors visualized by selective arteriography. It is suggested that the usefulness of these approaches will provide simpler means of screening and detecting pheochromocytoma.", "contents": "Diagnosis and localization of pheochromocytoma. Detection by measurement of urinary norepinephrine excretion during sleep, plasma norepinephrine concentration and computerized axial tomography (CT-scan). The feasibility of differentiating patients with pheochromocytoma from other hypertensive patients by measuring urinary excretion rates of norepinephrine during sleep, a period of physiologic suppression of norepinephrine release, was investigated. The mean excretion rates of norepinephrine in 248 normal subjects and in 109 patients with essential hypertension were 1.03 +/- 0.03 and 1.12 +/- 0.06 (SEM) micrograms/hour, respectively, whereas the lowest excretion rate among the six patients with pheochromocytoma was about seven times higher. Plasma norepinephrine concentration in patients with pheochromocytoma was also consistently above the range observed in both normotensive and hypertensive subjects. CT scan correctly identified the same tumors visualized by selective arteriography. It is suggested that the usefulness of these approaches will provide simpler means of screening and detecting pheochromocytoma."}
{"id": "PMID:463913", "title": "Essential hypertension in black and white subjects. Hemodynamic findings and fluid volume state.", "content": "Systemic hemodynamics (cardiac output, intraarterial pressure, total peripheral resistance) and intravascular volume (plasma volume and red cell mass) were measured in a population of 126 black and white patients, 51 with borderline hypertension and 75 with established essential hypertension. The findings were compared with those in 29 age-matched normotensive control subjects of both races. The white patients with established hypertension demonstrated a faster heart rate than the black patients (less than 0.05); this difference was more pronounced during upright tilt (p less than 0.02). No significant difference in cardiac index, total peripheral resistance, plasma volume or total blood volume was found between the two racial populations. Cardiac index correlated directly with plasma and total blood volume in black patients (r = 0.32, p less than 0.05) and white patients (r = 0.35, p less than 0.001) as well as in the whole study population (r = 0.36, p less than 0.001). The regression lines were similar in the two races. Further, a negative correlation was observed between the total peripheral resistance and plasma volume (r = -0.31, p less than 0.001) or total blood volume (r = -0.34, p less than 0.001), and it was similar in both races (blacks r = -0.48, p less than 0.01; whites r = -0.25, p less than 0.05). Age correlated significantly with total peripheral resistance in the white patients (r = 0.35, p less than 0.001) and in the total study population (r = 0.28, p less than 0.001). We conclude that, for every given age or level of arterial pressure, systemic hemodynamics are similar for the black and white patients with essential hypertension. These data, therefore, do not support the clinical impression that basic pathophysiology and hypertensive vascular disease are different in the black patient with essential hypertension.", "contents": "Essential hypertension in black and white subjects. Hemodynamic findings and fluid volume state. Systemic hemodynamics (cardiac output, intraarterial pressure, total peripheral resistance) and intravascular volume (plasma volume and red cell mass) were measured in a population of 126 black and white patients, 51 with borderline hypertension and 75 with established essential hypertension. The findings were compared with those in 29 age-matched normotensive control subjects of both races. The white patients with established hypertension demonstrated a faster heart rate than the black patients (less than 0.05); this difference was more pronounced during upright tilt (p less than 0.02). No significant difference in cardiac index, total peripheral resistance, plasma volume or total blood volume was found between the two racial populations. Cardiac index correlated directly with plasma and total blood volume in black patients (r = 0.32, p less than 0.05) and white patients (r = 0.35, p less than 0.001) as well as in the whole study population (r = 0.36, p less than 0.001). The regression lines were similar in the two races. Further, a negative correlation was observed between the total peripheral resistance and plasma volume (r = -0.31, p less than 0.001) or total blood volume (r = -0.34, p less than 0.001), and it was similar in both races (blacks r = -0.48, p less than 0.01; whites r = -0.25, p less than 0.05). Age correlated significantly with total peripheral resistance in the white patients (r = 0.35, p less than 0.001) and in the total study population (r = 0.28, p less than 0.001). We conclude that, for every given age or level of arterial pressure, systemic hemodynamics are similar for the black and white patients with essential hypertension. These data, therefore, do not support the clinical impression that basic pathophysiology and hypertensive vascular disease are different in the black patient with essential hypertension."}
{"id": "PMID:463915", "title": "The value of edema fluid protein measurement in patients with pulmonary edema.", "content": "Alveolar fluid and plasma proteins were analyzed in 24 patients with florid pulmonary edema, in 21 of whom pulmonary capillary wedge pressure (Pcw) was also measured. In all patients with Pcw less than 20 mm Hg, the edema fluid to plasma protein ratio exceeded 0.6; the mean edema fluid to plasma protein ratio in the four patients with cardiogenic edema (increased Pcw) was 0.46. In the 21 patients in whom full data were available, the net intravascular filtration force (Pcw - plasma colloid osmotic pressure) was less than -4 mm Hg, the value at which (according to others) pulmonary edema should occur, in only 10. When the interstitial colloid osmotic pressure, approximated by the osmotic pressure of edema fluid protein, was added, the net filtration force became positive in 17 of 21 patients. Comparison of the protein concentrations of edema fluid and plasma aids in the diagnostic separation of increased permeability from high hydrostatic pressure edema and adds to our understanding of the relative osmotic and hydrostatic forces that contribute to pulmonary edema when the alveolar-capillary membrane is damaged.", "contents": "The value of edema fluid protein measurement in patients with pulmonary edema. Alveolar fluid and plasma proteins were analyzed in 24 patients with florid pulmonary edema, in 21 of whom pulmonary capillary wedge pressure (Pcw) was also measured. In all patients with Pcw less than 20 mm Hg, the edema fluid to plasma protein ratio exceeded 0.6; the mean edema fluid to plasma protein ratio in the four patients with cardiogenic edema (increased Pcw) was 0.46. In the 21 patients in whom full data were available, the net intravascular filtration force (Pcw - plasma colloid osmotic pressure) was less than -4 mm Hg, the value at which (according to others) pulmonary edema should occur, in only 10. When the interstitial colloid osmotic pressure, approximated by the osmotic pressure of edema fluid protein, was added, the net filtration force became positive in 17 of 21 patients. Comparison of the protein concentrations of edema fluid and plasma aids in the diagnostic separation of increased permeability from high hydrostatic pressure edema and adds to our understanding of the relative osmotic and hydrostatic forces that contribute to pulmonary edema when the alveolar-capillary membrane is damaged."}
{"id": "PMID:463916", "title": "Community-acquired acinetobacter pneumonia.", "content": "Acinetobacter calcoaceticus var anitratus, a nonfermentative grampnegative bacillus, has been infrequently reported as a cause of community-acquired pneumonia. In this paper we describe the course of six recent patients with community-acquired, bacteremic pneumonia due to this organism and review the six previously reported cases. Our experience suggests this organism is a more common cause of community-acquired pneumonia than previously thought. Acinetobacter pneumonia occurs in older persons with chronic disease, especially alcoholism. It is a fulminant illness with respiratory distress, hypoxemia, leukopenia and shock. Chest roentgenograms reveal a lobar or bronchopneumonic infiltrate which often becomes bilateral within 24 hours of admission to the hospital. Pleural effusions are common. The mortality rate is 43 per cent. Factors that predict a fatal outcome are granulocytopenia, empyema and therapy with inappropriate antibiotics. Therapy with appropriate antibiotics, especially carbenicillin and an aminoglycoside, increases survival.", "contents": "Community-acquired acinetobacter pneumonia. Acinetobacter calcoaceticus var anitratus, a nonfermentative grampnegative bacillus, has been infrequently reported as a cause of community-acquired pneumonia. In this paper we describe the course of six recent patients with community-acquired, bacteremic pneumonia due to this organism and review the six previously reported cases. Our experience suggests this organism is a more common cause of community-acquired pneumonia than previously thought. Acinetobacter pneumonia occurs in older persons with chronic disease, especially alcoholism. It is a fulminant illness with respiratory distress, hypoxemia, leukopenia and shock. Chest roentgenograms reveal a lobar or bronchopneumonic infiltrate which often becomes bilateral within 24 hours of admission to the hospital. Pleural effusions are common. The mortality rate is 43 per cent. Factors that predict a fatal outcome are granulocytopenia, empyema and therapy with inappropriate antibiotics. Therapy with appropriate antibiotics, especially carbenicillin and an aminoglycoside, increases survival."}
{"id": "PMID:463917", "title": "Transfer factor therapy in the Wiskott-Aldrich syndrome. Results of long-term follow-up in 32 patients.", "content": "Thirty-two patients with the Wiskott-Aldrich syndrome have been treated with transfer factor provided by this laboratory. Apparent clinical benefit was observed in 44 per cent of them. The mean age of the patients who showed clinical benefit was significantly greater than that of the patients who showed no benefit. Conversion of immunologic reactivity correlated with clinical benefit. Thirteen of the patients who received transfer factor are alive, and 17 have died (43 per cent survival). Clinical benefit was correlated with survival. The median survival was greater than five years in the patients who showed clinical benefit, whereas it was 18 months in those who did not show clinical benefit. We conclude that transfer factor caused conversion of immunologic parameters, apparent clinical benefit and prolonged survival in some, but not all, patients with the Wiskott-Aldrich syndrome.", "contents": "Transfer factor therapy in the Wiskott-Aldrich syndrome. Results of long-term follow-up in 32 patients. Thirty-two patients with the Wiskott-Aldrich syndrome have been treated with transfer factor provided by this laboratory. Apparent clinical benefit was observed in 44 per cent of them. The mean age of the patients who showed clinical benefit was significantly greater than that of the patients who showed no benefit. Conversion of immunologic reactivity correlated with clinical benefit. Thirteen of the patients who received transfer factor are alive, and 17 have died (43 per cent survival). Clinical benefit was correlated with survival. The median survival was greater than five years in the patients who showed clinical benefit, whereas it was 18 months in those who did not show clinical benefit. We conclude that transfer factor caused conversion of immunologic parameters, apparent clinical benefit and prolonged survival in some, but not all, patients with the Wiskott-Aldrich syndrome."}
{"id": "PMID:463918", "title": "Abnormal regulation of antidiuretic hormone in idiopathic edema.", "content": "Idiopathic edema is characterized by impaired water excretion, particularly in the upright posture. Indirect evidence has shown that antidiuretic hormone is involved in this disease. For this reason, we measured urinary arginine vasopressin by radioimmunoassay before and during water loading (15 ml/kg) in 10 normal women and in 10 subjects with idiopathic edema in both the supine and upright postures. Daily sodium intake was 100 meq. Renin and aldosterone were concomitantly investigated, and abnormally high values were observed both in the recumbent and upright postures. Basal values for urinary arginine vasopressin were identical in control subjects and in patients with idiopathic edema. The water load significantly reduced urinary arginine vasopressin in normal women in both positions, but in those with idiopathic edema only in the supine position. In those with idiopathic edema, assumption of the upright posture was accompanied by a transient decrease in glomerular filtration, a major decrease in osmolar clearance and no decrease in urinary arginine vasopressin after water loading. Significant correlations were established between urinary arginine vasopressin and osmolar or volemic parameters in normal women, but these correlations were not found in those with idiopathic edema in either position. Arginine vasopressin regulation was abnormal in idiopathic edema, and this hormone was believed to play a part in the pathogenesis of this disease.", "contents": "Abnormal regulation of antidiuretic hormone in idiopathic edema. Idiopathic edema is characterized by impaired water excretion, particularly in the upright posture. Indirect evidence has shown that antidiuretic hormone is involved in this disease. For this reason, we measured urinary arginine vasopressin by radioimmunoassay before and during water loading (15 ml/kg) in 10 normal women and in 10 subjects with idiopathic edema in both the supine and upright postures. Daily sodium intake was 100 meq. Renin and aldosterone were concomitantly investigated, and abnormally high values were observed both in the recumbent and upright postures. Basal values for urinary arginine vasopressin were identical in control subjects and in patients with idiopathic edema. The water load significantly reduced urinary arginine vasopressin in normal women in both positions, but in those with idiopathic edema only in the supine position. In those with idiopathic edema, assumption of the upright posture was accompanied by a transient decrease in glomerular filtration, a major decrease in osmolar clearance and no decrease in urinary arginine vasopressin after water loading. Significant correlations were established between urinary arginine vasopressin and osmolar or volemic parameters in normal women, but these correlations were not found in those with idiopathic edema in either position. Arginine vasopressin regulation was abnormal in idiopathic edema, and this hormone was believed to play a part in the pathogenesis of this disease."}
{"id": "PMID:463919", "title": "Survival after recovery from acute myocardial infarction. Two and five year prognostic indices.", "content": "A prognostic index for two year survival following recovery from acute myocardial infarction has been verified in an independent group of 105 patients. Five variables comprise the index: systolic blood pressure level on admission, highest blood urea nitrogen level in the cardiac care unit; atrial arrhythmias in the cardiac care unit; angina pectoris for more than three months or a previous myocardial infarction; and more than one ventricular ectopic beat per hour recorded on an 8 hour dynamic electrocardiogram during convalescence just prior to hospital discharge. One hundred twenty-six patients have also been followed for five or more years, and we now report a five year prognostic index. Discriminant analysis indicates that the same five variables, although weighted differently, continue to be significant for prognostic assessment and may be utilized in the identification of patients at high and lower risk.", "contents": "Survival after recovery from acute myocardial infarction. Two and five year prognostic indices. A prognostic index for two year survival following recovery from acute myocardial infarction has been verified in an independent group of 105 patients. Five variables comprise the index: systolic blood pressure level on admission, highest blood urea nitrogen level in the cardiac care unit; atrial arrhythmias in the cardiac care unit; angina pectoris for more than three months or a previous myocardial infarction; and more than one ventricular ectopic beat per hour recorded on an 8 hour dynamic electrocardiogram during convalescence just prior to hospital discharge. One hundred twenty-six patients have also been followed for five or more years, and we now report a five year prognostic index. Discriminant analysis indicates that the same five variables, although weighted differently, continue to be significant for prognostic assessment and may be utilized in the identification of patients at high and lower risk."}
{"id": "PMID:463921", "title": "Hypertrophic hepatic osteoarthropathy. Clinical, roentgenologic, biochemical, hormonal and cardiorespiratory studies, and review of the literature.", "content": "Twenty patients with biopsy proved liver disease, and roentgenologic features of hypertrophic osteoarthropathy have been studied, and the literature has been reviewed. The syndrome is a rare association of many chronic liver diseases, including primary biliary cirrhosis, bile duct carcinoma, benign bile duct stricture, chronic active hepatitis, posthepatitic cirrhosis and alcoholic cirrhosis. Patients may be asymptomatic, although bone pain, arthralgia or arthritis may be presenting symptoms. Ninety per cent of the patients are clinical jaundiced at the time of diagnosis, and 95 per cent have digital clubbing. The distal tibia and fibula are the first bones to become involved, although wrist, foot bones, femurs, hand bones and humeri may be affected in order of frequency. There is no correlation between the presence of esophageal varices or surgical portacaval shunts and the extent of the syndrome, neither is there a correlation with the degree of liver function impairment. Serum calcium and phosphate levels are normal, as is urinary hydroxyproline and estrogen excretion. There was no evidence to implicate elevated levels of growth hormone or overdosage of vitamin A. Although the majority of patients tested had mild arterial hypoxemia, increased cardiac output and evidence of right to left shunting, these were also present in disease-matched control subjects without osteoarthropathy. For screening purposes, patients with chronic liver disease and clubbing should have roentgenologic studies of the lower tibias and fibulas, to select those patients suitable for a more extensive skeletal survey.", "contents": "Hypertrophic hepatic osteoarthropathy. Clinical, roentgenologic, biochemical, hormonal and cardiorespiratory studies, and review of the literature. Twenty patients with biopsy proved liver disease, and roentgenologic features of hypertrophic osteoarthropathy have been studied, and the literature has been reviewed. The syndrome is a rare association of many chronic liver diseases, including primary biliary cirrhosis, bile duct carcinoma, benign bile duct stricture, chronic active hepatitis, posthepatitic cirrhosis and alcoholic cirrhosis. Patients may be asymptomatic, although bone pain, arthralgia or arthritis may be presenting symptoms. Ninety per cent of the patients are clinical jaundiced at the time of diagnosis, and 95 per cent have digital clubbing. The distal tibia and fibula are the first bones to become involved, although wrist, foot bones, femurs, hand bones and humeri may be affected in order of frequency. There is no correlation between the presence of esophageal varices or surgical portacaval shunts and the extent of the syndrome, neither is there a correlation with the degree of liver function impairment. Serum calcium and phosphate levels are normal, as is urinary hydroxyproline and estrogen excretion. There was no evidence to implicate elevated levels of growth hormone or overdosage of vitamin A. Although the majority of patients tested had mild arterial hypoxemia, increased cardiac output and evidence of right to left shunting, these were also present in disease-matched control subjects without osteoarthropathy. For screening purposes, patients with chronic liver disease and clubbing should have roentgenologic studies of the lower tibias and fibulas, to select those patients suitable for a more extensive skeletal survey."}
{"id": "PMID:463922", "title": "How to be less invasive.", "content": "Unless cardiac output is reduced, alveolar ventilation can be monitored without arterial blood gas analysis by estimating arterial carbon dioxide tension (PaO2) from rebreathing measurement of mixed venous carbon dioxide tension (PvCO2) (PaCO2 = 0.8 PvCO2). If cardiac output is reduced, the PvCO2 - PaCO2 difference increases, reflecting the increased venoarterial carbon dioxide (CO2) content difference (Fick principle). A reduction in cardiac output can thus be quantified without catheterization of the central circulation by measuring both PaCO2 and PvCO2. The significance of such a reduction in cardiac output for oxygen (O2) delivery to tissues is determined by calculation of mixed venous O2 saturation (SvO2), using the inter-relationship of CO2 production to O2 consumption. With normal arterial oxygenation (SaO2) and hemoglobin concentration, PaCO2 less than 0.8 PvCO2 - 12 implies a reduction in cardiac output such that SvO2 is less than 33 per cent, which indicates inadequate O2 delivery to tissues. Ear oximetry and rebreathing measurement of PvCO2 are simple, noninvasive, bedside techniques.", "contents": "How to be less invasive. Unless cardiac output is reduced, alveolar ventilation can be monitored without arterial blood gas analysis by estimating arterial carbon dioxide tension (PaO2) from rebreathing measurement of mixed venous carbon dioxide tension (PvCO2) (PaCO2 = 0.8 PvCO2). If cardiac output is reduced, the PvCO2 - PaCO2 difference increases, reflecting the increased venoarterial carbon dioxide (CO2) content difference (Fick principle). A reduction in cardiac output can thus be quantified without catheterization of the central circulation by measuring both PaCO2 and PvCO2. The significance of such a reduction in cardiac output for oxygen (O2) delivery to tissues is determined by calculation of mixed venous O2 saturation (SvO2), using the inter-relationship of CO2 production to O2 consumption. With normal arterial oxygenation (SaO2) and hemoglobin concentration, PaCO2 less than 0.8 PvCO2 - 12 implies a reduction in cardiac output such that SvO2 is less than 33 per cent, which indicates inadequate O2 delivery to tissues. Ear oximetry and rebreathing measurement of PvCO2 are simple, noninvasive, bedside techniques."}
{"id": "PMID:463924", "title": "Marrow granulocyte reserves in black Americans. Hydrocortisone-induced granulocytosis in the \"benign\" neutropenia of the black.", "content": "The bone marrow granulocyte reserves of nine black patients with \"benign\" neutropenia were estimated by measuring the maximum neutrophil increment after the administration of hydrocortisone. Thirty control subjects, including 16 black and 14 white adults, were also studied. The mean neutrophil increment in the black patients with neutropenia was significantly less than that in the control subjects. The mean increment in the black control was also significantly less than that in the white control subjects. Four of the 16 black control subjects had neutrophil counts below 2,000/microliter; if these four are excluded from the analysis, the difference between the black and white control subjects is no longer significant. These data suggest that there is a subpopulation of healthy black adults with neutrophil counts below 2,000/microliter with reduced marrow granulocyte reserves as tested by corticosteroids. Bone marrow aspirates in four of the neutropenic patients showed normal cellularity and myeloid maturation suggesting that the lower increments are due to a difference in granulocyte release rather than to a difference in granulocyte production.", "contents": "Marrow granulocyte reserves in black Americans. Hydrocortisone-induced granulocytosis in the \"benign\" neutropenia of the black. The bone marrow granulocyte reserves of nine black patients with \"benign\" neutropenia were estimated by measuring the maximum neutrophil increment after the administration of hydrocortisone. Thirty control subjects, including 16 black and 14 white adults, were also studied. The mean neutrophil increment in the black patients with neutropenia was significantly less than that in the control subjects. The mean increment in the black control was also significantly less than that in the white control subjects. Four of the 16 black control subjects had neutrophil counts below 2,000/microliter; if these four are excluded from the analysis, the difference between the black and white control subjects is no longer significant. These data suggest that there is a subpopulation of healthy black adults with neutrophil counts below 2,000/microliter with reduced marrow granulocyte reserves as tested by corticosteroids. Bone marrow aspirates in four of the neutropenic patients showed normal cellularity and myeloid maturation suggesting that the lower increments are due to a difference in granulocyte release rather than to a difference in granulocyte production."}
{"id": "PMID:463926", "title": "Anaerobic bacterial meningitis.", "content": "Anaerobic meningitis occurred in four patients in whom anaerobic bacteria had not been suspected as a possible cause. The predisposing conditions were typical of those seen in patients previously reported to have this infection and included chronic otitis media with mastoiditis, chronic sinusitis, recent craniotomy and abdominal trauma. Two of the patients had undergone immunosuppression (immunosuppressed patients); a compromised immune system may facilitate the development of anaerobic meningitis in patients with the appropritate underlying conditions. Head and neck neoplasms, head trauma, suppurative pharyngitis and laminectomy wounds are additional situations in which anaerobic meningitis occurs. Anaerobic bacterial meningitis probably occurs more often than is recognized. The cerebrospinal fluid should be transported and cultured anaerobically when meningitis develops in a patient with a predisposing condition.", "contents": "Anaerobic bacterial meningitis. Anaerobic meningitis occurred in four patients in whom anaerobic bacteria had not been suspected as a possible cause. The predisposing conditions were typical of those seen in patients previously reported to have this infection and included chronic otitis media with mastoiditis, chronic sinusitis, recent craniotomy and abdominal trauma. Two of the patients had undergone immunosuppression (immunosuppressed patients); a compromised immune system may facilitate the development of anaerobic meningitis in patients with the appropritate underlying conditions. Head and neck neoplasms, head trauma, suppurative pharyngitis and laminectomy wounds are additional situations in which anaerobic meningitis occurs. Anaerobic bacterial meningitis probably occurs more often than is recognized. The cerebrospinal fluid should be transported and cultured anaerobically when meningitis develops in a patient with a predisposing condition."}
{"id": "PMID:463927", "title": "Corynebacterium pseudotuberculosis. A new cause of infectious and eosinophilic pneumonia.", "content": "A 28 year old veterinary medical student experienced spiking fever, cough, peripheral blood eosinophilia and an eosinophilic pulmonary infiltrate. Corynebacterium pseudotuberculosis was isolated from a transtracheal aspirate and bronchoscopy washings. C. pseudotuberculosis, a pathogen responsible for lymphadenitis in livestock, has never been reported to cause pneumonia in man. In the four cases of C. pseudotuberculosis previously reported, lymphadenitis was the chief clinical presentation. In our patient specific antibodies against the isolated C. pseudotuberculosis developed but not against the other corynebacteria. With erythromycin therapy, the peripheral blood eosinophilia and IgE anti-C. pseudotuberculosis titer decreased whereas the IgG titer continued to increase.", "contents": "Corynebacterium pseudotuberculosis. A new cause of infectious and eosinophilic pneumonia. A 28 year old veterinary medical student experienced spiking fever, cough, peripheral blood eosinophilia and an eosinophilic pulmonary infiltrate. Corynebacterium pseudotuberculosis was isolated from a transtracheal aspirate and bronchoscopy washings. C. pseudotuberculosis, a pathogen responsible for lymphadenitis in livestock, has never been reported to cause pneumonia in man. In the four cases of C. pseudotuberculosis previously reported, lymphadenitis was the chief clinical presentation. In our patient specific antibodies against the isolated C. pseudotuberculosis developed but not against the other corynebacteria. With erythromycin therapy, the peripheral blood eosinophilia and IgE anti-C. pseudotuberculosis titer decreased whereas the IgG titer continued to increase."}
{"id": "PMID:463928", "title": "Fever and pulmonary thromboembolism.", "content": "Fever patterns associated with pulmonary thromboembolism have not been well characterized. Upon review of 35 consecutive patients with angiographically documented pulmonary emboli, fever was present in 24 patients; and in 20, it was attributed solely to pulmonary thromboembolism. Analysis of these cases indicates that high fever (temperature greater than 39 degrees C) due to pulmonary thromboembolism may occur early, and low-grade fever may continue for a week or more. Fever persisting beyond six days, however, especially with temperatures over 38.5 degrees C, should not be ascribed to pulmonary thromboembolism unless other causes have been carefully excluded. If the clinical setting and patient's findings are consistent with pulmonary thromboembolism, one should not be deterred from presumptively making this diagnosis and initiating therapy because of the presence of high fever.", "contents": "Fever and pulmonary thromboembolism. Fever patterns associated with pulmonary thromboembolism have not been well characterized. Upon review of 35 consecutive patients with angiographically documented pulmonary emboli, fever was present in 24 patients; and in 20, it was attributed solely to pulmonary thromboembolism. Analysis of these cases indicates that high fever (temperature greater than 39 degrees C) due to pulmonary thromboembolism may occur early, and low-grade fever may continue for a week or more. Fever persisting beyond six days, however, especially with temperatures over 38.5 degrees C, should not be ascribed to pulmonary thromboembolism unless other causes have been carefully excluded. If the clinical setting and patient's findings are consistent with pulmonary thromboembolism, one should not be deterred from presumptively making this diagnosis and initiating therapy because of the presence of high fever."}
{"id": "PMID:463929", "title": "Dysautonomia in mitral valve prolapse.", "content": "Many of the clinical features of patients with mitral valve prolapse can logically be attributed to abnormal autonomic neural function. Accordingly, we have studied heart rate and blood pressure response to a standardized Valsalva maneuver and postural test in 44 untreated patients with demonstrated mitral valve prolapse. Fifteen healthy subjects of similar age served as controls. The directional changes of blood pressure and heart rate were similar in control subjects and patients in both tests, but patients differed from control subjects by their widely oscillating heart rate during the upright posture, and their exaggerated and prolonged bradycardia during the recovery phase of the Valsalva maneuver and following their return to recumbency in the postural test. This bradycardia persisted for 30 to 90 seconds after blood pressure returned to control values. Patients also showed a greater respiratory variation of R-R interval, which became especially marked during the adjustment to changes of posture. We postulate an abnormal central modulation of baroreflexes as the best explanation for the dysautonomic responses of symptomatic patients with prolapsed mitral valves.", "contents": "Dysautonomia in mitral valve prolapse. Many of the clinical features of patients with mitral valve prolapse can logically be attributed to abnormal autonomic neural function. Accordingly, we have studied heart rate and blood pressure response to a standardized Valsalva maneuver and postural test in 44 untreated patients with demonstrated mitral valve prolapse. Fifteen healthy subjects of similar age served as controls. The directional changes of blood pressure and heart rate were similar in control subjects and patients in both tests, but patients differed from control subjects by their widely oscillating heart rate during the upright posture, and their exaggerated and prolonged bradycardia during the recovery phase of the Valsalva maneuver and following their return to recumbency in the postural test. This bradycardia persisted for 30 to 90 seconds after blood pressure returned to control values. Patients also showed a greater respiratory variation of R-R interval, which became especially marked during the adjustment to changes of posture. We postulate an abnormal central modulation of baroreflexes as the best explanation for the dysautonomic responses of symptomatic patients with prolapsed mitral valves."}
{"id": "PMID:463930", "title": "Renal stones in Wilson's disease.", "content": "Fifty-four patients with Wilson's disease were studied with regard to renal stones. Seven of the 45 patients (16 per cent) who underwent roentgenographic procedures of the urinary tract had unequivocal evidence of renal stones. In four of the seven patients with Wilson's disease who had renal stones, the stones were discovered at the time or before the diagnosis of Wilson's disease was made. Of the several possible factors that may predispose patients with Wilson's disease to renal stone formation, the renal tubular acidosis pattern of abnormality in acid-base excretion is probably the most significant. In general, patients with renal stones and unexplained neurologic, bony or hepatic abnormalities should be screened for Wilson's disease by slit-lamp examination, determination of serum copper and ceruloplasmin concentrations, and urinary excretion of copper, particularly if they have relatively alkaline urine.", "contents": "Renal stones in Wilson's disease. Fifty-four patients with Wilson's disease were studied with regard to renal stones. Seven of the 45 patients (16 per cent) who underwent roentgenographic procedures of the urinary tract had unequivocal evidence of renal stones. In four of the seven patients with Wilson's disease who had renal stones, the stones were discovered at the time or before the diagnosis of Wilson's disease was made. Of the several possible factors that may predispose patients with Wilson's disease to renal stone formation, the renal tubular acidosis pattern of abnormality in acid-base excretion is probably the most significant. In general, patients with renal stones and unexplained neurologic, bony or hepatic abnormalities should be screened for Wilson's disease by slit-lamp examination, determination of serum copper and ceruloplasmin concentrations, and urinary excretion of copper, particularly if they have relatively alkaline urine."}
{"id": "PMID:463935", "title": "Articular manifestations of polymyositis and dermatomyositis.", "content": "Nine patients with polymyositis or dermatomyositis associated with arthritis without antinuclear antibodies have been studied. In eight patients a mildly inflammatory nonerosive arthritis occurred coincident with early manifestations or before the appearance of symptomatic muscle disease. Hands, wrists and knees were prominently involved. This arthritis responded to corticosteroids given for the myositis. One patient had erosive arthritis and periarticular calcifications associated with acute inflammation that seemed to diminish with colchicine therapy. Synovial biopsy specimens showed surface fibrin deposition, focal loss of lining cells and mild inflammation--findings similar to those in scleroderma. By electron microscopy tubuloreticular structures were found in vascular endothelium in synovium, as in other tissues in polymyositis. All patients surprisingly had pulmonary manifestations suggesting the possibility that the condition in this group of patients may represent a distinctive subset of polymyositis.", "contents": "Articular manifestations of polymyositis and dermatomyositis. Nine patients with polymyositis or dermatomyositis associated with arthritis without antinuclear antibodies have been studied. In eight patients a mildly inflammatory nonerosive arthritis occurred coincident with early manifestations or before the appearance of symptomatic muscle disease. Hands, wrists and knees were prominently involved. This arthritis responded to corticosteroids given for the myositis. One patient had erosive arthritis and periarticular calcifications associated with acute inflammation that seemed to diminish with colchicine therapy. Synovial biopsy specimens showed surface fibrin deposition, focal loss of lining cells and mild inflammation--findings similar to those in scleroderma. By electron microscopy tubuloreticular structures were found in vascular endothelium in synovium, as in other tissues in polymyositis. All patients surprisingly had pulmonary manifestations suggesting the possibility that the condition in this group of patients may represent a distinctive subset of polymyositis."}
{"id": "PMID:463937", "title": "Antibody response to infection in multiple myeloma. Implications for vaccination.", "content": "We report the humoral immune response of a patient with multiple myeloma to rapidly successive episodes of meningococcal and pneumococcal meningitis. Specific antibody responses included a high bactericidal titer (1:640) against the infecting meningococcus and a sharp increase (from 198 to 8,097 ng/ml antibody nitrogen) in antibody to the type-specific capsular polysaccharide of the infecting pneumococcus. These data, showing the production of protective antibodies against the two pathogens, suggest that some patients with multiple myeloma might also respond to appropriately administered bacterial vaccines. This fact should be ascertained because vaccination could potentially reduce the high rate of bacterial infections associated with this disease.", "contents": "Antibody response to infection in multiple myeloma. Implications for vaccination. We report the humoral immune response of a patient with multiple myeloma to rapidly successive episodes of meningococcal and pneumococcal meningitis. Specific antibody responses included a high bactericidal titer (1:640) against the infecting meningococcus and a sharp increase (from 198 to 8,097 ng/ml antibody nitrogen) in antibody to the type-specific capsular polysaccharide of the infecting pneumococcus. These data, showing the production of protective antibodies against the two pathogens, suggest that some patients with multiple myeloma might also respond to appropriately administered bacterial vaccines. This fact should be ascertained because vaccination could potentially reduce the high rate of bacterial infections associated with this disease."}
{"id": "PMID:463938", "title": "Rapid onset of emphysema associated with diffuse parenchymal disease.", "content": "In experimental models pulmonary emphysema may be produced in hours to days; however, in human subjects emphysema commonly develops over a period of many years. In this report we document a case of severe emphysema which developed in less than six months in association with an unexplained illness characterized by dyspnea, hypoxemia and bilateral lung parenchymal disease. There was no hereditary predisposition. The diffuse alveolar injury syndrome must be considered as a potential cause of alveolar disruption.", "contents": "Rapid onset of emphysema associated with diffuse parenchymal disease. In experimental models pulmonary emphysema may be produced in hours to days; however, in human subjects emphysema commonly develops over a period of many years. In this report we document a case of severe emphysema which developed in less than six months in association with an unexplained illness characterized by dyspnea, hypoxemia and bilateral lung parenchymal disease. There was no hereditary predisposition. The diffuse alveolar injury syndrome must be considered as a potential cause of alveolar disruption."}
{"id": "PMID:463939", "title": "Legionnaires' disease. A cause of severe abscess-forming pneumonia.", "content": "In two previously well nonsmokers fatal pneumonia developed with extensive abscess formation. Legionnaires' bacillus was the only pathogen isolated. These cases indicate that Legionnaires' bacillus is capable of causing extensive necrosis of the lung.", "contents": "Legionnaires' disease. A cause of severe abscess-forming pneumonia. In two previously well nonsmokers fatal pneumonia developed with extensive abscess formation. Legionnaires' bacillus was the only pathogen isolated. These cases indicate that Legionnaires' bacillus is capable of causing extensive necrosis of the lung."}
{"id": "PMID:463940", "title": "Electrophysiologic studies of perphenazine and protriptyline in a patient with psychotropic drug-induced ventricular fibrillation.", "content": "A 51 year old woman sustained ventricular fibrillation while receiving perphenazine and protriptyline. After successful resuscitation and clinical stabilization, cardiac electrophysiologic studies were performed before and after the administration of each of these medications. Perphenazine widened the ventricular echo zone and facilitated induction of short salvoes of ventricular tachycardia (repetitive ventricular response). Protriptyline also widened the ventricular echo zone and allowed easy induction of long runs of ventricular tachycardia. Both psychotropic agents increased the incidence of ventricular dysrhythmias in this patient. The electrophysiologic study is a useful technique in determining the interaction between psychotropic drugs and life-threatening arrhythmias; it may provide a means of identifying the patients with cardiac disease in whom administration of these agents may be fatal.", "contents": "Electrophysiologic studies of perphenazine and protriptyline in a patient with psychotropic drug-induced ventricular fibrillation. A 51 year old woman sustained ventricular fibrillation while receiving perphenazine and protriptyline. After successful resuscitation and clinical stabilization, cardiac electrophysiologic studies were performed before and after the administration of each of these medications. Perphenazine widened the ventricular echo zone and facilitated induction of short salvoes of ventricular tachycardia (repetitive ventricular response). Protriptyline also widened the ventricular echo zone and allowed easy induction of long runs of ventricular tachycardia. Both psychotropic agents increased the incidence of ventricular dysrhythmias in this patient. The electrophysiologic study is a useful technique in determining the interaction between psychotropic drugs and life-threatening arrhythmias; it may provide a means of identifying the patients with cardiac disease in whom administration of these agents may be fatal."}
{"id": "PMID:463941", "title": "Virus myocarditis: a critique of the literature from clinical, electrocardiographic, and pathologic standpoints.", "content": "Concurrent viral infection and myocarditis presumably indicate viral myocarditis. The electrocardiographic and pathologic changes developing during acute infection may, however, result from changes not produced by the infection itself, eg, fever, tachycardia, ischemia, potassium depletion, vitamin deficiencies, drugs. This qualification should be remembered in the evaluation of all alleged virus myocarditis. Viral infection seems to prefer the very young. Its localization in the heart is favored by general or local hypoxia, perhaps thus explaining a predilection for the subendocardium. It may be influenced by the strain of the organism or by the hormonal or immunologic state of the host. Intrauterine infection of the fetus with rubella, mumps, and perhaps coxsackievirus can induce congenital cardiac defects. The role of virus infection in precipitating acute myocardial infarction deserves further study. The value of treatment, including steroids, nonsteroidal immunosuppressive agents, and \"antiviral\" agents is not yet established.", "contents": "Virus myocarditis: a critique of the literature from clinical, electrocardiographic, and pathologic standpoints. Concurrent viral infection and myocarditis presumably indicate viral myocarditis. The electrocardiographic and pathologic changes developing during acute infection may, however, result from changes not produced by the infection itself, eg, fever, tachycardia, ischemia, potassium depletion, vitamin deficiencies, drugs. This qualification should be remembered in the evaluation of all alleged virus myocarditis. Viral infection seems to prefer the very young. Its localization in the heart is favored by general or local hypoxia, perhaps thus explaining a predilection for the subendocardium. It may be influenced by the strain of the organism or by the hormonal or immunologic state of the host. Intrauterine infection of the fetus with rubella, mumps, and perhaps coxsackievirus can induce congenital cardiac defects. The role of virus infection in precipitating acute myocardial infarction deserves further study. The value of treatment, including steroids, nonsteroidal immunosuppressive agents, and \"antiviral\" agents is not yet established."}
{"id": "PMID:463942", "title": "Turner's syndrome and carbohydrate metabolism. I. Impaired insulin secretion after tolbutamide and glucagon stimulation tests: evidence of insulin deficiency.", "content": "Tolbutamide (25 mg/kg: maximum 1 mg) intravenously (IV) and glucagon (0.03 mg/kg; maximum 1 mg) intramuscularly (IM) were given sequentially to 12 untreated girls with XO-Turner's syndrome (ages 6.5 to 17.0 years) and to ten female siblings (ages 8.0 to 16.7 years) to evaluate blood sugar (BS), plasma free fatty acids (FFA), serum immunoreactive insulin (IRI), and growth hormone (IRGH) responses to these insulinogenic secretagogues in order to appreciate any differences of genotypes on carbohydrate metabolism within identical family backgrounds. Seven of 12 patients with Turner's syndrome (58%) but none of the siblings were 20% or more overweight for height. There was a family history of diabetes mellitus in 7 to 12 patients (58%). The results showed significant elevations of mean FFA levels and decreased mean IRI responses to both insulinogenic stimuli without differences in mean BS or serum IRGH responses in the Turner's syndrome patients when compared to the controls. Three of 12 patients (25%) had abnormally elevated and prolonged blood sugar responses to IM glucagon. These findings show a significant incidence of abnormal carbohydrate and lipid metabolism and insulin deficiency in untreated patients with XO-Turner's syndrome when compared to normal female siblings and implicate this chromosomal defect in the impaired insulin secretion.", "contents": "Turner's syndrome and carbohydrate metabolism. I. Impaired insulin secretion after tolbutamide and glucagon stimulation tests: evidence of insulin deficiency. Tolbutamide (25 mg/kg: maximum 1 mg) intravenously (IV) and glucagon (0.03 mg/kg; maximum 1 mg) intramuscularly (IM) were given sequentially to 12 untreated girls with XO-Turner's syndrome (ages 6.5 to 17.0 years) and to ten female siblings (ages 8.0 to 16.7 years) to evaluate blood sugar (BS), plasma free fatty acids (FFA), serum immunoreactive insulin (IRI), and growth hormone (IRGH) responses to these insulinogenic secretagogues in order to appreciate any differences of genotypes on carbohydrate metabolism within identical family backgrounds. Seven of 12 patients with Turner's syndrome (58%) but none of the siblings were 20% or more overweight for height. There was a family history of diabetes mellitus in 7 to 12 patients (58%). The results showed significant elevations of mean FFA levels and decreased mean IRI responses to both insulinogenic stimuli without differences in mean BS or serum IRGH responses in the Turner's syndrome patients when compared to the controls. Three of 12 patients (25%) had abnormally elevated and prolonged blood sugar responses to IM glucagon. These findings show a significant incidence of abnormal carbohydrate and lipid metabolism and insulin deficiency in untreated patients with XO-Turner's syndrome when compared to normal female siblings and implicate this chromosomal defect in the impaired insulin secretion."}
{"id": "PMID:463943", "title": "Turner's syndrome and carbohydrate metabolism. II. Parotid salivary insulin concentration in normal subjects and in patients with gonadal dysgenesis.", "content": "Ten children with XO gonadal dysgenesis and ten control siblings (CS) had sequential IV tolbutamide and IM glucagon tests to ascertain serum and salivary insulin concentrations, to confirm the presence of parotid salivary insulin and to determine if these concentrations were of diagnostic value in the diagnosis of insulin deficiency. After tolbutamide, peak serum insulin concentrations were lower in the patients with Turner's syndrome (TS) than in control siblings (58 +/- 10 vs 90 +/- 15 microU/ml) and fractional areas under insulin curves were significantly lower in the patients with Turner's syndrome at 10 to 15 minutes (TS: 240 +/- 16 microUmin/ml; CS: 340 +/- 46 microU-min/ml, P less than 0.05) and at 15 to 30 minutes (TS: 562 +/- 62 microU-min/ml; CS: 884 +/- 128 microU-min/ml, P less than 0.05). After glucagon, peak serum insulin concentrations were significantly lower in Turner's syndrome than in control siblings(P less than 0.02, at 45 minutes) and fractional areas under insulin curves were also lower in TS than in siblings at 30 to 45 minutes (TS: 1,062 +/- 185 microU-min/ml; CS: 2,189 +/- 402 microU-min/ml, P less than 0.02). Basal salivary immunoreactive insulin (IR) concentrations were similar in both groups: TS: 4.8 +/- 2.1 microU/min; CS: 2.1 +/- 0.4 microU/min. Peak salivary IRI concentrations after tolbutamide were 13.8 +/- 4.7 microU/min in Turner's syndrome and 8.8 +/- 1.8 microU/ml in control siblings. Peak salivary IRI values in Turner's syndrome and in control siblings after glucagon were 26.8 +/- 10.1 and 13.4 +/- 2.1 microU/min, respectively. While significant differces in insulin secretion in serum were detected in the two patient groups, no differences were noted between groups when salivary insulin concentrations were compared. These data confirm serum insulin deficiency in gonadal dysgenesis, the presence of immunoreactive insulin in parotid saliva, and suggest the possibility that extrapancreatic insulin synthesis could occur in the parotid gland.", "contents": "Turner's syndrome and carbohydrate metabolism. II. Parotid salivary insulin concentration in normal subjects and in patients with gonadal dysgenesis. Ten children with XO gonadal dysgenesis and ten control siblings (CS) had sequential IV tolbutamide and IM glucagon tests to ascertain serum and salivary insulin concentrations, to confirm the presence of parotid salivary insulin and to determine if these concentrations were of diagnostic value in the diagnosis of insulin deficiency. After tolbutamide, peak serum insulin concentrations were lower in the patients with Turner's syndrome (TS) than in control siblings (58 +/- 10 vs 90 +/- 15 microU/ml) and fractional areas under insulin curves were significantly lower in the patients with Turner's syndrome at 10 to 15 minutes (TS: 240 +/- 16 microUmin/ml; CS: 340 +/- 46 microU-min/ml, P less than 0.05) and at 15 to 30 minutes (TS: 562 +/- 62 microU-min/ml; CS: 884 +/- 128 microU-min/ml, P less than 0.05). After glucagon, peak serum insulin concentrations were significantly lower in Turner's syndrome than in control siblings(P less than 0.02, at 45 minutes) and fractional areas under insulin curves were also lower in TS than in siblings at 30 to 45 minutes (TS: 1,062 +/- 185 microU-min/ml; CS: 2,189 +/- 402 microU-min/ml, P less than 0.02). Basal salivary immunoreactive insulin (IR) concentrations were similar in both groups: TS: 4.8 +/- 2.1 microU/min; CS: 2.1 +/- 0.4 microU/min. Peak salivary IRI concentrations after tolbutamide were 13.8 +/- 4.7 microU/min in Turner's syndrome and 8.8 +/- 1.8 microU/ml in control siblings. Peak salivary IRI values in Turner's syndrome and in control siblings after glucagon were 26.8 +/- 10.1 and 13.4 +/- 2.1 microU/min, respectively. While significant differces in insulin secretion in serum were detected in the two patient groups, no differences were noted between groups when salivary insulin concentrations were compared. These data confirm serum insulin deficiency in gonadal dysgenesis, the presence of immunoreactive insulin in parotid saliva, and suggest the possibility that extrapancreatic insulin synthesis could occur in the parotid gland."}
{"id": "PMID:463950", "title": "Modern management of the breech delivery.", "content": "Retrospective review of 457 breech deliveries failed to reveal a significant improvement in neonatal mortality and morbidity rates for those delivered by cesarean section vs. vaginal delivery. A slight but insignificant improvement in salvage was found for the 1,000 to 1,500 gram weight category when delivery was by cesarean section. Most of the deaths in the less than 1,500 gram breech presentation were associated with hyaline membrane disease or congenital defects incompatible with life and sepsis rather than directly related to the manner of breech delivery.", "contents": "Modern management of the breech delivery. Retrospective review of 457 breech deliveries failed to reveal a significant improvement in neonatal mortality and morbidity rates for those delivered by cesarean section vs. vaginal delivery. A slight but insignificant improvement in salvage was found for the 1,000 to 1,500 gram weight category when delivery was by cesarean section. Most of the deaths in the less than 1,500 gram breech presentation were associated with hyaline membrane disease or congenital defects incompatible with life and sepsis rather than directly related to the manner of breech delivery."}
{"id": "PMID:463944", "title": "Carbohydrate metabolism in porphyria cutanea tarda.", "content": "Porphyria cutanea tarda (PCT) has a known increased incidence of diabetes mellitus and hepatic involvement. We investigated glucose tolerance and glucoregulatory hormone alterations in seven patients with PCT and correlated these results with hepatic histology by percutaneous liver biopsy. Abnormal glucose tolerance was observed in six of the seven patients (87%). Fasting serum insulin levels were normal range, and normal glucose and growth hormone responses to standard, exogenous intravenous insulin were observed. Fasting serum glucagon and urine free cortisol levels were normal in those patients in whom they were measured. While varying degrees of abnormalities were found on histopathologic exam of the liver biopsies, no patient met the criteria for cirrhosis, and none of the patients demonstrated abnormal levels of insulin counterregulatory hormones commonly seen in cirrhosis. Thus, liver disease may not be the sole cause of the observed glucose intolerance and hyperinsulinemia in PCT patients.", "contents": "Carbohydrate metabolism in porphyria cutanea tarda. Porphyria cutanea tarda (PCT) has a known increased incidence of diabetes mellitus and hepatic involvement. We investigated glucose tolerance and glucoregulatory hormone alterations in seven patients with PCT and correlated these results with hepatic histology by percutaneous liver biopsy. Abnormal glucose tolerance was observed in six of the seven patients (87%). Fasting serum insulin levels were normal range, and normal glucose and growth hormone responses to standard, exogenous intravenous insulin were observed. Fasting serum glucagon and urine free cortisol levels were normal in those patients in whom they were measured. While varying degrees of abnormalities were found on histopathologic exam of the liver biopsies, no patient met the criteria for cirrhosis, and none of the patients demonstrated abnormal levels of insulin counterregulatory hormones commonly seen in cirrhosis. Thus, liver disease may not be the sole cause of the observed glucose intolerance and hyperinsulinemia in PCT patients."}
{"id": "PMID:463951", "title": "Vaginal breech delivery or cesarean section.", "content": "In 579 singleton breech deliveries the total perinatal mortality rate was 10.4% and the corrected perinatal mortality rate was 0.67%. There were 452 babies delivered vaginally (78.1%) and 127 delivered by cesarean section (21.9%). The perinatal mortality rate was very high in the premature breech delivery and in the low-birth-weight infant. Cesarean section should be used to avoid a traumatic vaginal delivery and it should be used more liberally in the mature breech, but it is unlikely that cesarean section rates in excess of 50% will significantly reduce the perinatal mortality mortality rate. More information is required as to whether extending the indications for cesarean section in the low-birth-weight infant will further reduce the perinatal mortality rate.", "contents": "Vaginal breech delivery or cesarean section. In 579 singleton breech deliveries the total perinatal mortality rate was 10.4% and the corrected perinatal mortality rate was 0.67%. There were 452 babies delivered vaginally (78.1%) and 127 delivered by cesarean section (21.9%). The perinatal mortality rate was very high in the premature breech delivery and in the low-birth-weight infant. Cesarean section should be used to avoid a traumatic vaginal delivery and it should be used more liberally in the mature breech, but it is unlikely that cesarean section rates in excess of 50% will significantly reduce the perinatal mortality mortality rate. More information is required as to whether extending the indications for cesarean section in the low-birth-weight infant will further reduce the perinatal mortality rate."}
{"id": "PMID:463946", "title": "Lack of effect of mealtime wine on the hypoprothrombinemia of oral anticoagulants.", "content": "To evaluate the effect of mealtime wine of the hypoprothrombinemic effect of anticoagulant therapy, eight normal subjects were anticoagulated at therapeutic levels for 21 days. Eight experiments were performed with racemic warfarin alone, 7 with warfarin plus a total of 10 oz mealtime wine per day ingested, and 6 with warfarin plus a total of 20 oz wine  per day. Each subject served as his own control. After day 3, the average one-stage prothrombin activity was 33%, 33%, and 32% and the average warfarin level was 2.9, 2.9, and 3.1 microgram/ml for warfarin alone, warfarin plus 10 oz of wine, and warfarin plus 20 oz of wine, respectively. It is concluded that 10 and even 20 oz of daily mealtime wine has no effect on therapeutic hypoprothrombinemia.", "contents": "Lack of effect of mealtime wine on the hypoprothrombinemia of oral anticoagulants. To evaluate the effect of mealtime wine of the hypoprothrombinemic effect of anticoagulant therapy, eight normal subjects were anticoagulated at therapeutic levels for 21 days. Eight experiments were performed with racemic warfarin alone, 7 with warfarin plus a total of 10 oz mealtime wine per day ingested, and 6 with warfarin plus a total of 20 oz wine  per day. Each subject served as his own control. After day 3, the average one-stage prothrombin activity was 33%, 33%, and 32% and the average warfarin level was 2.9, 2.9, and 3.1 microgram/ml for warfarin alone, warfarin plus 10 oz of wine, and warfarin plus 20 oz of wine, respectively. It is concluded that 10 and even 20 oz of daily mealtime wine has no effect on therapeutic hypoprothrombinemia."}
{"id": "PMID:463952", "title": "Cellular and humoral immune status in women with chronic Candida vaginitis.", "content": "The cellular and humoral immune responses of nine women with chronic Candida vaginitis and 29 control subjects were evaluated. Agglutinating and precipitating antibodies were studied and tests of lymphocyte transformation, lymphokine production, and cutaneous delayed hypersensitivity were performed. The results suggest a lack of cellular immune response to Candida antigens in women with chronic Candida vaginitis.", "contents": "Cellular and humoral immune status in women with chronic Candida vaginitis. The cellular and humoral immune responses of nine women with chronic Candida vaginitis and 29 control subjects were evaluated. Agglutinating and precipitating antibodies were studied and tests of lymphocyte transformation, lymphokine production, and cutaneous delayed hypersensitivity were performed. The results suggest a lack of cellular immune response to Candida antigens in women with chronic Candida vaginitis."}
{"id": "PMID:463953", "title": "A randomized prospective study of the use-effectiveness of two methods of natural family planning: an interim report.", "content": "Data accumulated to date from the Los Angeles study indicate that the total termination rate for 12 months from the beginning of the training period and from formal entry into the study was high for the ovulation method (OM), and symptothermal method (STM). Voluntary withdrawal was the highest single reason for termination in both methods. The 12 month voluntary withdrawal rate, measured from formal entry into the study, was significantly higher for OM users. Pregnancy rates measured from both the beginning of training and formal entry into the study were significantly higher for OM users than for STM users. Complete analysis of the data collected during the study is currently in progress. It is anticipated that some of the causes for the differences in pregnancy rates and withdrawal rates between the two methods can be identified.", "contents": "A randomized prospective study of the use-effectiveness of two methods of natural family planning: an interim report. Data accumulated to date from the Los Angeles study indicate that the total termination rate for 12 months from the beginning of the training period and from formal entry into the study was high for the ovulation method (OM), and symptothermal method (STM). Voluntary withdrawal was the highest single reason for termination in both methods. The 12 month voluntary withdrawal rate, measured from formal entry into the study, was significantly higher for OM users. Pregnancy rates measured from both the beginning of training and formal entry into the study were significantly higher for OM users than for STM users. Complete analysis of the data collected during the study is currently in progress. It is anticipated that some of the causes for the differences in pregnancy rates and withdrawal rates between the two methods can be identified."}
{"id": "PMID:463948", "title": "Case report. Legionnaires' disease. A review of clinical aspects of 18 sporadic cases.", "content": "An increased incidence of sporadic or isolated cases of Legionnaires' disease has been identified. A 68-year-old male with lobar pneumonia is reported in detail and clinical aspects of 17 other cases from the literature are summarized. Important epidemiologic factors remain to be resolved, but a pattern of diagnostic criteria, clinical course, and therapeutic response similar to the epidemic incidence emerges from this collected series to guide the alert clinician.", "contents": "Case report. Legionnaires' disease. A review of clinical aspects of 18 sporadic cases. An increased incidence of sporadic or isolated cases of Legionnaires' disease has been identified. A 68-year-old male with lobar pneumonia is reported in detail and clinical aspects of 17 other cases from the literature are summarized. Important epidemiologic factors remain to be resolved, but a pattern of diagnostic criteria, clinical course, and therapeutic response similar to the epidemic incidence emerges from this collected series to guide the alert clinician."}
{"id": "PMID:463954", "title": "Human chorionic gonadotropin beta-subunit--like immunoreactive material in the plasma of women wearing an intrauterine progesterone contraceptive system.", "content": "A systematic study of the presence of human chorionic gonadotropin beta-subunit--like (hCG beta-like) material in the plasma of one woman sampled daily throughout one menstrual cycle and in nine women studied on a selected bleeding schedule has been carried out. Peaks of immunoreactive hCG beta-like activity were found in the follicular and luteal phases of these menstrual cycles which could not be explained by a cross-reaction of luteinizing hormone (LH) in the plasma. The same nine women were studied again 1 month after the insertion of the intrauterine progesterone contraceptive system (IPCS) and again 6 to 8 months later. Although the presence of hCG beta-like immunoactivity correlated with LH values in the cycles studied, there was a significant decrease in its presence 1 month after IPCS use and a highly significant decrease after 6 to 8 months of use.", "contents": "Human chorionic gonadotropin beta-subunit--like immunoreactive material in the plasma of women wearing an intrauterine progesterone contraceptive system. A systematic study of the presence of human chorionic gonadotropin beta-subunit--like (hCG beta-like) material in the plasma of one woman sampled daily throughout one menstrual cycle and in nine women studied on a selected bleeding schedule has been carried out. Peaks of immunoreactive hCG beta-like activity were found in the follicular and luteal phases of these menstrual cycles which could not be explained by a cross-reaction of luteinizing hormone (LH) in the plasma. The same nine women were studied again 1 month after the insertion of the intrauterine progesterone contraceptive system (IPCS) and again 6 to 8 months later. Although the presence of hCG beta-like immunoactivity correlated with LH values in the cycles studied, there was a significant decrease in its presence 1 month after IPCS use and a highly significant decrease after 6 to 8 months of use."}
{"id": "PMID:463949", "title": "Case report. Clinical manifestations and treatment of Legionnaires' disease.", "content": "Eight patients with atypical pneumonia caused by the Legionnaires' disease organism were seen during the spring and summer of 1977. Two died of the acute illness. All patients were febrile and presented with symptoms of acute respiratory infection. Other symptoms included malaise, anorexia, chills, myalgia, and headache. Severe hypoxemia was a striking feature. Conventional methods to determine the etiology of these pneumonias were unsuccessful but subsequent serological studies confirmed the diagnosis of Legionnaires' disease. Seven patients were treated with beta-lactam antibiotics alone or with an aminoglycoside and all failed to respond. Six were subsequently treated with erythromycin and five who received this drug for at least 48 hours were markedly improved within this time period. We believe that erythromycin is effective in the treatment of Legionnaires' disease.", "contents": "Case report. Clinical manifestations and treatment of Legionnaires' disease. Eight patients with atypical pneumonia caused by the Legionnaires' disease organism were seen during the spring and summer of 1977. Two died of the acute illness. All patients were febrile and presented with symptoms of acute respiratory infection. Other symptoms included malaise, anorexia, chills, myalgia, and headache. Severe hypoxemia was a striking feature. Conventional methods to determine the etiology of these pneumonias were unsuccessful but subsequent serological studies confirmed the diagnosis of Legionnaires' disease. Seven patients were treated with beta-lactam antibiotics alone or with an aminoglycoside and all failed to respond. Six were subsequently treated with erythromycin and five who received this drug for at least 48 hours were markedly improved within this time period. We believe that erythromycin is effective in the treatment of Legionnaires' disease."}
{"id": "PMID:463955", "title": "Human hexosaminidase isozymes. IV. Effects of oral contraceptive steroids on serum hexosaminidase activity.", "content": "Data from a mass screening program for identification of persons who are heterozygous for Tay-Sachs disease have been analyzed for the effects of oral contraceptive steroids on the activity in serum of hexosaminidase (hex) and the various hex isozymes. Women using oral contraceptives show a significantly higher total serum hex activity, reflecting mainly an increase in the heat-stable hex isozyme (hex I), and a smaller increase in the heat-labile hex A than do women using no medications. The changes in women using oral contraceptives are qualitatively similar to those observed during pregnancy. The distribution of responses to oral contraceptives is unimodal, and some of the variations may be related to differences in amount of steroid ingested. In addition, underlying genetic variation may contribute to the observed differences in enzyme activities.", "contents": "Human hexosaminidase isozymes. IV. Effects of oral contraceptive steroids on serum hexosaminidase activity. Data from a mass screening program for identification of persons who are heterozygous for Tay-Sachs disease have been analyzed for the effects of oral contraceptive steroids on the activity in serum of hexosaminidase (hex) and the various hex isozymes. Women using oral contraceptives show a significantly higher total serum hex activity, reflecting mainly an increase in the heat-stable hex isozyme (hex I), and a smaller increase in the heat-labile hex A than do women using no medications. The changes in women using oral contraceptives are qualitatively similar to those observed during pregnancy. The distribution of responses to oral contraceptives is unimodal, and some of the variations may be related to differences in amount of steroid ingested. In addition, underlying genetic variation may contribute to the observed differences in enzyme activities."}
{"id": "PMID:463956", "title": "Hypothalamic-pituitary responses to high concentrations of gonadal steroids in a three-month-old infant with a feminizing gonadal stromal tumor.", "content": "A 3-month-old female infant feminized by an ovarian stromal tumor is presented. Clinicopathologic aspects of such tumors occurring in infancy are discussed. In addition, the evaluation of hypothalamic-pituitary function preoperatively and postoperatively are presented. In the preoperative, high-steroid environment, both basal and stimulated secretion of follicle-stimulating hormone (FSH) was suppressed. Secretion of luteinizing hormone (LH) was normal. Following surgical castration, stimulated secretion of both gonadotropins clearly increased. These findings suggest an active but less sensitive negative feedback mechanism for FSH than for LH during infancy, i.e., high concentrations of gonadal steroids are necessary to maximally suppress FSH secretion while normal steroid concentrations appear to maximally suppress LH secretion. Developmental changes in hypothalamic-pituitary sensitivity to negative feedback controls are discussed in light of these findings.", "contents": "Hypothalamic-pituitary responses to high concentrations of gonadal steroids in a three-month-old infant with a feminizing gonadal stromal tumor. A 3-month-old female infant feminized by an ovarian stromal tumor is presented. Clinicopathologic aspects of such tumors occurring in infancy are discussed. In addition, the evaluation of hypothalamic-pituitary function preoperatively and postoperatively are presented. In the preoperative, high-steroid environment, both basal and stimulated secretion of follicle-stimulating hormone (FSH) was suppressed. Secretion of luteinizing hormone (LH) was normal. Following surgical castration, stimulated secretion of both gonadotropins clearly increased. These findings suggest an active but less sensitive negative feedback mechanism for FSH than for LH during infancy, i.e., high concentrations of gonadal steroids are necessary to maximally suppress FSH secretion while normal steroid concentrations appear to maximally suppress LH secretion. Developmental changes in hypothalamic-pituitary sensitivity to negative feedback controls are discussed in light of these findings."}
{"id": "PMID:463947", "title": "Parenteral prophylaxis against enterococcal endocarditis.", "content": "Three commonly used antibiotic regimens for the prevention of enterococcal endocarditis were administered parenterally to six healthy men in a crossover manner. The regimens included 1 gm of streptomycin intramuscularly (IM) in combination with (1) procaine penicillin 600,000 units plus aqueous penicillin G 200,000 units IM; or (2) ampicillin 25 mg/kg intravenously (IV); or (3) ampicillin 1 gm IM. The combinations containing ampicillin IM or IV with  streptomycin produced bactericidal activity at dilutions of 1:2 or greater for the majority of the strains, whereas the penicillin-streptomycin regimen did not. All regimens were poorly bactericidal against three strains of enterococci which were highly resistant to streptomycin. These data suggest that ampicillin plus streptomycin is the preferred regimen for prophylaxis.", "contents": "Parenteral prophylaxis against enterococcal endocarditis. Three commonly used antibiotic regimens for the prevention of enterococcal endocarditis were administered parenterally to six healthy men in a crossover manner. The regimens included 1 gm of streptomycin intramuscularly (IM) in combination with (1) procaine penicillin 600,000 units plus aqueous penicillin G 200,000 units IM; or (2) ampicillin 25 mg/kg intravenously (IV); or (3) ampicillin 1 gm IM. The combinations containing ampicillin IM or IV with  streptomycin produced bactericidal activity at dilutions of 1:2 or greater for the majority of the strains, whereas the penicillin-streptomycin regimen did not. All regimens were poorly bactericidal against three strains of enterococci which were highly resistant to streptomycin. These data suggest that ampicillin plus streptomycin is the preferred regimen for prophylaxis."}
{"id": "PMID:463957", "title": "Premature rupture of the membranes: a conservative approach.", "content": "A conservative approach was followed in 188 patients with premature rupture of the membranes (PROM) over a 2 year period. There were no neonatal deaths from sepsis, and only one infant showed a positive central bacteriologic culture. Fifty-seven percent of all patients less than 37 weeks' gestation had a latent period of 24 hours and 19% went beyond 7 days before labor began. Patients less than 34 weeks' gestation who received antepartum steroids for lung maturation showed no increased infectious morbidity or mortality. A conservative approach to patients with PROM seems reasonable when either prematurity and/or a cervix unfavorable for induction further complicates the pregnancy.", "contents": "Premature rupture of the membranes: a conservative approach. A conservative approach was followed in 188 patients with premature rupture of the membranes (PROM) over a 2 year period. There were no neonatal deaths from sepsis, and only one infant showed a positive central bacteriologic culture. Fifty-seven percent of all patients less than 37 weeks' gestation had a latent period of 24 hours and 19% went beyond 7 days before labor began. Patients less than 34 weeks' gestation who received antepartum steroids for lung maturation showed no increased infectious morbidity or mortality. A conservative approach to patients with PROM seems reasonable when either prematurity and/or a cervix unfavorable for induction further complicates the pregnancy."}
{"id": "PMID:463958", "title": "Thalassemia and pregnancy: results of an antenatal screening program.", "content": "A thalassemia screening program was implemented at our institution using the finding of a mean corpuscular volume less than 80 fl as the index of abnormality. Further evaluation using hemoglobin (Hb) electrophoresis and serum iron studies was carried out according to the scheme detailed below. A diagnosis of thalassemia was made in 33 women (42 pregnancies). Eight patients had alpha-thalassemia trait, 23 beta-thalassemia trait, and two Hb H disease. Thalassemia trait did not have any adverse effect on pregnancy outcome. In two couples the fetuses were at risk for homozygous disease and in one couple the fetus was at risk for sickle cell beta-thalassemia. The screening program described is an effective and inexpensive means of detecting thalassemia in an antenatal population and is applicable to most every clinic or office setting.", "contents": "Thalassemia and pregnancy: results of an antenatal screening program. A thalassemia screening program was implemented at our institution using the finding of a mean corpuscular volume less than 80 fl as the index of abnormality. Further evaluation using hemoglobin (Hb) electrophoresis and serum iron studies was carried out according to the scheme detailed below. A diagnosis of thalassemia was made in 33 women (42 pregnancies). Eight patients had alpha-thalassemia trait, 23 beta-thalassemia trait, and two Hb H disease. Thalassemia trait did not have any adverse effect on pregnancy outcome. In two couples the fetuses were at risk for homozygous disease and in one couple the fetus was at risk for sickle cell beta-thalassemia. The screening program described is an effective and inexpensive means of detecting thalassemia in an antenatal population and is applicable to most every clinic or office setting."}
{"id": "PMID:463959", "title": "Maternal and amniotic fluid steroids throughout human pregnancy.", "content": "Concentrations of testosterone, dihydrotestosterone, androstenedione, progesterone, 17 alpha-hydroxyprogesterone, and estradiol were measured by radioimmunoassay in the amniotic fluid and maternal peripheral blood obtained from normal pregnancies between 14 and 40 weeks of gestation. There was a sex difference in the levels of all the androgenic steroids in the amniotic fluid before 20 weeks with higher levels in pregnancies with male fetuses. Amniotic fluid 17 alpha-hydroxyprogesterone levels were significantly elevated in a pregnancy with the fetus affected with congenital adrenal hyperplasia. The levels of all the steroids in the amniotic fluid were significantly elevated in the pregnancy with molar degeneration of the placenta. There was a sex difference in the levels of dihydrotestosterone in the maternal peripheral blood before 20 weeks with higher levels in pregnancies with male fetuses. There was no correlation between the steroid levels in the maternal serum and amniotic fluid even though most of the samples of maternal serum were drawn at the same time as amniocentesis.", "contents": "Maternal and amniotic fluid steroids throughout human pregnancy. Concentrations of testosterone, dihydrotestosterone, androstenedione, progesterone, 17 alpha-hydroxyprogesterone, and estradiol were measured by radioimmunoassay in the amniotic fluid and maternal peripheral blood obtained from normal pregnancies between 14 and 40 weeks of gestation. There was a sex difference in the levels of all the androgenic steroids in the amniotic fluid before 20 weeks with higher levels in pregnancies with male fetuses. Amniotic fluid 17 alpha-hydroxyprogesterone levels were significantly elevated in a pregnancy with the fetus affected with congenital adrenal hyperplasia. The levels of all the steroids in the amniotic fluid were significantly elevated in the pregnancy with molar degeneration of the placenta. There was a sex difference in the levels of dihydrotestosterone in the maternal peripheral blood before 20 weeks with higher levels in pregnancies with male fetuses. There was no correlation between the steroid levels in the maternal serum and amniotic fluid even though most of the samples of maternal serum were drawn at the same time as amniocentesis."}
{"id": "PMID:463960", "title": "Effect of stress during labor on the concentration of cortisol and estriol in maternal plasma.", "content": "The effect of stress during labor on the plasma concentration of cortisol, unconjugated estriol, and human chorionic somatomammotropin was studied in 15 healthy primiparous women. According to the method of pain relief the parturients were divided into two groups. One group was given continuous epidural analgesia and the other group received pethidine, diazepam, and nitrous oxide/oxygen in response to pain. In the most stressed nonepidural group there was a significant rise in the cortisol level during labor and the estriol concentration fell significantly. In the epidural group no significant changes were observed with respect to the concentration of either cortisol or estriol. These results give further support to the hypothesis that severe maternal stress may lead to a reduced concentration of estriol in maternal plasma.", "contents": "Effect of stress during labor on the concentration of cortisol and estriol in maternal plasma. The effect of stress during labor on the plasma concentration of cortisol, unconjugated estriol, and human chorionic somatomammotropin was studied in 15 healthy primiparous women. According to the method of pain relief the parturients were divided into two groups. One group was given continuous epidural analgesia and the other group received pethidine, diazepam, and nitrous oxide/oxygen in response to pain. In the most stressed nonepidural group there was a significant rise in the cortisol level during labor and the estriol concentration fell significantly. In the epidural group no significant changes were observed with respect to the concentration of either cortisol or estriol. These results give further support to the hypothesis that severe maternal stress may lead to a reduced concentration of estriol in maternal plasma."}
{"id": "PMID:463961", "title": "Short-term cycles in human fetal activity. I. Normal pregnancies.", "content": "Recordings of fetal motor activity in utero were carried out on 17 women with normal pregnancies at 23 to 37 weeks' gestation. Fetal motility was monitored by a method based on integrative EMG recordings derived through electrodes attached to maternal abdominal surface. Orthogonal spectral analysis revealed 40 minute and 80 minute cycles in fetal motility in 59% of the fetuses. The correlation between objective and subjective (maternal self-assessment) scores of fetal activity was generally confirmed. Methods of monitoring fetal activity, based on short-term recordings, are criticized in the light of the present results.", "contents": "Short-term cycles in human fetal activity. I. Normal pregnancies. Recordings of fetal motor activity in utero were carried out on 17 women with normal pregnancies at 23 to 37 weeks' gestation. Fetal motility was monitored by a method based on integrative EMG recordings derived through electrodes attached to maternal abdominal surface. Orthogonal spectral analysis revealed 40 minute and 80 minute cycles in fetal motility in 59% of the fetuses. The correlation between objective and subjective (maternal self-assessment) scores of fetal activity was generally confirmed. Methods of monitoring fetal activity, based on short-term recordings, are criticized in the light of the present results."}
{"id": "PMID:463964", "title": "Collection and use of autologous fetal blood.", "content": "A prospective study was completed to evaluate the feasibility of autologous fetal blood collection. Following puncture on the umbilical vein, fetal blood was drawn into sterile heparinized plastic syringes and aliquots were subjected to coagulation and culture studies. None of the blood samples exhibited significant growth of bacterial pathogens and all patients had normal coagulation studies at 24 hours of age. These data demonstrate that fetal blood can be safely collected and given to infants subjected to shock or iatrogenic blood loss.", "contents": "Collection and use of autologous fetal blood. A prospective study was completed to evaluate the feasibility of autologous fetal blood collection. Following puncture on the umbilical vein, fetal blood was drawn into sterile heparinized plastic syringes and aliquots were subjected to coagulation and culture studies. None of the blood samples exhibited significant growth of bacterial pathogens and all patients had normal coagulation studies at 24 hours of age. These data demonstrate that fetal blood can be safely collected and given to infants subjected to shock or iatrogenic blood loss."}
{"id": "PMID:463972", "title": "Human chorionic gonadotropin--like material: presence in normal human tissues.", "content": "With the use of radioreceptor assay for gonadotropin and a beta-chain radioimmunoassay for human chorionic gonadotropin (hCG), we have been able to demonstrate the presence of hCG-like material in all normal human tissues tested. This activity was completely absorbed by a hCHG-antibody affinity column and was demonstrated to have altered carbohydrate content by studies showing this material did not bind to concanavalin A (con A)-Sepharose affinity columns. In identical studies greater than 90% of placental hCG was bound to con A. However, if placental hCG is rendered carbohydrate free it also does not bind to con A. It is known that carbohydrate-free hCG is cleared rapidly from the circulation and thus possesses little or no biological potency in vivo. Accordingly, we hypothesize: (1) that this hCG-like material in normal tissues has the protein structure of hCG but does not possess the carbohydrate moieties of placental hCG and probably has little or no bioactivity in vivo and (2) that the trophoblastic cell is not unique in its ability to synthesize hCG but has developed the ability to glycosylate hCG, transforming a ubiquitous cellular protein into a hormone, hCG might better be called human cellular gonadotropin.", "contents": "Human chorionic gonadotropin--like material: presence in normal human tissues. With the use of radioreceptor assay for gonadotropin and a beta-chain radioimmunoassay for human chorionic gonadotropin (hCG), we have been able to demonstrate the presence of hCG-like material in all normal human tissues tested. This activity was completely absorbed by a hCHG-antibody affinity column and was demonstrated to have altered carbohydrate content by studies showing this material did not bind to concanavalin A (con A)-Sepharose affinity columns. In identical studies greater than 90% of placental hCG was bound to con A. However, if placental hCG is rendered carbohydrate free it also does not bind to con A. It is known that carbohydrate-free hCG is cleared rapidly from the circulation and thus possesses little or no biological potency in vivo. Accordingly, we hypothesize: (1) that this hCG-like material in normal tissues has the protein structure of hCG but does not possess the carbohydrate moieties of placental hCG and probably has little or no bioactivity in vivo and (2) that the trophoblastic cell is not unique in its ability to synthesize hCG but has developed the ability to glycosylate hCG, transforming a ubiquitous cellular protein into a hormone, hCG might better be called human cellular gonadotropin."}
{"id": "PMID:463973", "title": "Platelet life span in pregnancies resulting in small-for-gestational age infants.", "content": "Platelet number and life span were determined in the last trimester of pregnancy in 22 women who were delivered of small-for-gestational age (SGA) infants and in 21 women with infants having normal birth weights. Platelet life span was determined by means of a nonradioisotopic method involving the use of acetylsalicylic acid. Mean platelet life span was 7.2 days in women with SGA infants, which is significantly shorter than the 9.2 days found in the control group. The mean platelet count in the SGA group was 235 x 10(9)/L, whereas in the control group it was 208 x 10(9)/L. These data indicate the existence of a compensated thrombocytolytic state in pregnancies with insufficient fetal growth. This could be explained by an increased platelet consumption in the uteroplacental arterial bed.", "contents": "Platelet life span in pregnancies resulting in small-for-gestational age infants. Platelet number and life span were determined in the last trimester of pregnancy in 22 women who were delivered of small-for-gestational age (SGA) infants and in 21 women with infants having normal birth weights. Platelet life span was determined by means of a nonradioisotopic method involving the use of acetylsalicylic acid. Mean platelet life span was 7.2 days in women with SGA infants, which is significantly shorter than the 9.2 days found in the control group. The mean platelet count in the SGA group was 235 x 10(9)/L, whereas in the control group it was 208 x 10(9)/L. These data indicate the existence of a compensated thrombocytolytic state in pregnancies with insufficient fetal growth. This could be explained by an increased platelet consumption in the uteroplacental arterial bed."}
{"id": "PMID:463975", "title": "Evaluation of abnormal cervical cytology during pregnancy with colposcopy.", "content": "During a 6 1/2 year study period the authors used colposcopy to evaluate 174 pregnant patients with abnormal cervical cytology. The technique of colposcopy proved safe, accurate, and effective in determining the presence or absence of cervical cancer in the present patient's cervix. Colposcopy is mandatory for the evaluation of abnormal cervical cytology during pregancy.", "contents": "Evaluation of abnormal cervical cytology during pregnancy with colposcopy. During a 6 1/2 year study period the authors used colposcopy to evaluate 174 pregnant patients with abnormal cervical cytology. The technique of colposcopy proved safe, accurate, and effective in determining the presence or absence of cervical cancer in the present patient's cervix. Colposcopy is mandatory for the evaluation of abnormal cervical cytology during pregancy."}
{"id": "PMID:463976", "title": "The effect of pregnancy on the cervical squamocolumnar junction in patients with abnormal cervical cytology.", "content": "During a 6 1/2 year study period the author used colposcopy to evaluate 174 pregnant patients with abnormal cervical cytology. Of these patients, 42% had an exocervical squamocolumnar junction. In those patients beyond the age of 25, or with a parity greater than 1, 80% had endocervical junctions. The predominant effect of pregnancy is to stimulate endocervical migration of this junction.", "contents": "The effect of pregnancy on the cervical squamocolumnar junction in patients with abnormal cervical cytology. During a 6 1/2 year study period the author used colposcopy to evaluate 174 pregnant patients with abnormal cervical cytology. Of these patients, 42% had an exocervical squamocolumnar junction. In those patients beyond the age of 25, or with a parity greater than 1, 80% had endocervical junctions. The predominant effect of pregnancy is to stimulate endocervical migration of this junction."}
{"id": "PMID:463977", "title": "The role of glucocorticoids, unstressful labor, and atraumatic delivery in the prevention of respiratory distress syndrome.", "content": "A 37 month review of the effects of glucocorticoids and uncompromised labor with atraumatic delivery on the incidence of respiratory distress syndrome (RDS) is presented. The incidence of RDS in the control and study population is not significantly different; however, the incidence of RDS in the control and study groups is markedly lower than the published figures for the same age and weight range. The low incidence of RDS in the control population is attributed to the avoidance of intrapartum hypoxia and stress during an uncompromised labor and atraumatic delivery.", "contents": "The role of glucocorticoids, unstressful labor, and atraumatic delivery in the prevention of respiratory distress syndrome. A 37 month review of the effects of glucocorticoids and uncompromised labor with atraumatic delivery on the incidence of respiratory distress syndrome (RDS) is presented. The incidence of RDS in the control and study population is not significantly different; however, the incidence of RDS in the control and study groups is markedly lower than the published figures for the same age and weight range. The low incidence of RDS in the control population is attributed to the avoidance of intrapartum hypoxia and stress during an uncompromised labor and atraumatic delivery."}
{"id": "PMID:463978", "title": "Estriol determinations in diabetic pregnancies complicated by nephropathy.", "content": "In order to study the effect of renal disease on urinary estriol clearance rate, we have measured the concentrations of plasma and urinary estriol (E3) in pregnancies of 69 diabetic patients, 25 of whom had nephropathy. No correlation was found between endogenous creatinine clearance (CCr) and estriol clearance (CE3) rates (r = 0.07), and mean CE3 of diabetic patients with diminished CCr (less than 100 ml/min) was not significantly different from that of patients with normal CCr (greater than or equal to 100 ml/min). The ratios between total, unconjugated estriol and urinary estriol concentrations in patients with diminished CCr were not different from those patients with normal CCr. Cases where high plasma estriol and low urinary estriol concentrations coexisted were not found. It is concluded that in this group of diabetic patients, diminished CCr had no discernible effect on urinary CE3 possibly because renal tubular function remained intact. In patients with diabetic nephropathy either urinary or plasma E3 could be used to assess fetoplacental function.", "contents": "Estriol determinations in diabetic pregnancies complicated by nephropathy. In order to study the effect of renal disease on urinary estriol clearance rate, we have measured the concentrations of plasma and urinary estriol (E3) in pregnancies of 69 diabetic patients, 25 of whom had nephropathy. No correlation was found between endogenous creatinine clearance (CCr) and estriol clearance (CE3) rates (r = 0.07), and mean CE3 of diabetic patients with diminished CCr (less than 100 ml/min) was not significantly different from that of patients with normal CCr (greater than or equal to 100 ml/min). The ratios between total, unconjugated estriol and urinary estriol concentrations in patients with diminished CCr were not different from those patients with normal CCr. Cases where high plasma estriol and low urinary estriol concentrations coexisted were not found. It is concluded that in this group of diabetic patients, diminished CCr had no discernible effect on urinary CE3 possibly because renal tubular function remained intact. In patients with diabetic nephropathy either urinary or plasma E3 could be used to assess fetoplacental function."}
{"id": "PMID:463979", "title": "Continuous variability of fetal PO2 in the chronically catheterized fetal sheep.", "content": "A method of continuous monitoring of fetal intravascular PO2 at various sites in the circulation in the chronically catheterized fetal sheep for up to 41 days (mean 11.1 days) has been compared with values obtained in blood samples measured extracorporeally in a standard blood gas analyzer. A double-blind comparison of the two methods showed that there was no bias between the two methods and correlation was 0.94. The stability of the electrodes was superior to that of a conventional blood gas analyzer. In every animal there was continuous variability of fetal vascular PO2. In the period from 105 to 126 days' gestation we noted the presence of slow increases in basal uterine tone that we have called \"contractures\". The frequency of these contractures was very regular at approximately one per hour. The frequency of these contracutres was very regular at approximately one per hour. There is a statistically significant related fall in fetal vascular PO2 in relation to these contractures. Well-coordinated uterine contractions during labor also produced a fall in fetal vascular PO2 that was related to the uterine activity.", "contents": "Continuous variability of fetal PO2 in the chronically catheterized fetal sheep. A method of continuous monitoring of fetal intravascular PO2 at various sites in the circulation in the chronically catheterized fetal sheep for up to 41 days (mean 11.1 days) has been compared with values obtained in blood samples measured extracorporeally in a standard blood gas analyzer. A double-blind comparison of the two methods showed that there was no bias between the two methods and correlation was 0.94. The stability of the electrodes was superior to that of a conventional blood gas analyzer. In every animal there was continuous variability of fetal vascular PO2. In the period from 105 to 126 days' gestation we noted the presence of slow increases in basal uterine tone that we have called \"contractures\". The frequency of these contractures was very regular at approximately one per hour. The frequency of these contracutres was very regular at approximately one per hour. There is a statistically significant related fall in fetal vascular PO2 in relation to these contractures. Well-coordinated uterine contractions during labor also produced a fall in fetal vascular PO2 that was related to the uterine activity."}
{"id": "PMID:463980", "title": "The relationship between prematurely ruptured membranes and fetal immunoglobulin production.", "content": "Cord blood and maternal sera were studied in a series of 227 cases of prematurely ruptured membranes (PRM) with respect to: (1) fetal immunoglobulin (lg) synthesis associated with PRM, (2) the interrelationship between different lg classes during infection, and (3) the relationship between lg values and the duration of PRM prior to the onset of labor. A preliminary report from this laboratory, which indicated that a humorla fetal immune response occurred in some but not all cases of PRM, and that significant increases in either IgA or IgM could be found, was confirmed. There was both clinical and immunologic evidence of one peak of infection one to 12 hours after onset of PRM and another after 72 hours after onset of PRM, suggesting that some patients were infected before the clinical onset of PRM symptomatology. Increased IgA and/or IgM was found in 16.3% of infants with clinical evidence of infection. This was comparable to the 18.5% of patients with PRM who had elevated IgA and/or IgM without clinical evidence of infection. Further, there was no correlation between the severity of infection and the presence of lg elevation. Based on the data in the present series, lg determination in cord blood cannot be used to distinguish cases of PRM with and without fetal infection.", "contents": "The relationship between prematurely ruptured membranes and fetal immunoglobulin production. Cord blood and maternal sera were studied in a series of 227 cases of prematurely ruptured membranes (PRM) with respect to: (1) fetal immunoglobulin (lg) synthesis associated with PRM, (2) the interrelationship between different lg classes during infection, and (3) the relationship between lg values and the duration of PRM prior to the onset of labor. A preliminary report from this laboratory, which indicated that a humorla fetal immune response occurred in some but not all cases of PRM, and that significant increases in either IgA or IgM could be found, was confirmed. There was both clinical and immunologic evidence of one peak of infection one to 12 hours after onset of PRM and another after 72 hours after onset of PRM, suggesting that some patients were infected before the clinical onset of PRM symptomatology. Increased IgA and/or IgM was found in 16.3% of infants with clinical evidence of infection. This was comparable to the 18.5% of patients with PRM who had elevated IgA and/or IgM without clinical evidence of infection. Further, there was no correlation between the severity of infection and the presence of lg elevation. Based on the data in the present series, lg determination in cord blood cannot be used to distinguish cases of PRM with and without fetal infection."}
{"id": "PMID:463982", "title": "Ovarian carcinoma: improved survival following abdominopelvic irradiation in patients with a completed pelvic operation.", "content": "A prospective, stratified, randomized study of 190 postoperative ovarian patients with Stages IB, II, and III (asymptomatic) presentations is reported. The median time of follow-up was 52 months. Patients in whom bilateral salpingo-oophorectomy and hysterectomy (BSOH) could not be completed because of extensive pelvic tumor had a poor prognosis which did not differ for any of the therapied tested. When BSOH was completed, pelvic plus abdominopelvic irradiation (P + AB) with no diaphragmatic shielding significnatly improved patient survival rate and long-term control of occult upper abdominal disease in approximately 25% more patients than pelvic irradiation alone or followed by adjuvant daily chlorambucil therapy. The effectiveness of P + AB in BSOH-completed patients was independent of stage or tumor grade and was most clearly appreciated in patients with all gross tumor removed. Chlorambucil added to pelvic irradiation delayed the time to treatment failure without reducing the number of treatment failures.", "contents": "Ovarian carcinoma: improved survival following abdominopelvic irradiation in patients with a completed pelvic operation. A prospective, stratified, randomized study of 190 postoperative ovarian patients with Stages IB, II, and III (asymptomatic) presentations is reported. The median time of follow-up was 52 months. Patients in whom bilateral salpingo-oophorectomy and hysterectomy (BSOH) could not be completed because of extensive pelvic tumor had a poor prognosis which did not differ for any of the therapied tested. When BSOH was completed, pelvic plus abdominopelvic irradiation (P + AB) with no diaphragmatic shielding significnatly improved patient survival rate and long-term control of occult upper abdominal disease in approximately 25% more patients than pelvic irradiation alone or followed by adjuvant daily chlorambucil therapy. The effectiveness of P + AB in BSOH-completed patients was independent of stage or tumor grade and was most clearly appreciated in patients with all gross tumor removed. Chlorambucil added to pelvic irradiation delayed the time to treatment failure without reducing the number of treatment failures."}
{"id": "PMID:463984", "title": "Induction of preterm labor in the rat by antiprogesterone.", "content": "The validity of a new concept, predicting that preterm labor can be induced without oxytocic stimulation by a regulatory imbalance, generated by antiprogesterone (A-P)-provoked P withdrawal (Pw), has been examined in the rat model. At day 19 of pregnancy a single oral dose of the steroidal A-O isoxazol, which inhibits P synthesis, significantly reduced uterine P levels, increased uterine estradiol and prostaglandin F levels, and induced preterm labor. This precocious regulatory imbalance and preterm labor were prevented, by blocking the A-P-induced Pw with P treatment. The regulatory imbalance which triggered the onset of spontaneous labor in the control animals was similar, but it occurred at term rather than before term. The potential of this method in improving the management of medically indicated induction of preterm labor is discussed.", "contents": "Induction of preterm labor in the rat by antiprogesterone. The validity of a new concept, predicting that preterm labor can be induced without oxytocic stimulation by a regulatory imbalance, generated by antiprogesterone (A-P)-provoked P withdrawal (Pw), has been examined in the rat model. At day 19 of pregnancy a single oral dose of the steroidal A-O isoxazol, which inhibits P synthesis, significantly reduced uterine P levels, increased uterine estradiol and prostaglandin F levels, and induced preterm labor. This precocious regulatory imbalance and preterm labor were prevented, by blocking the A-P-induced Pw with P treatment. The regulatory imbalance which triggered the onset of spontaneous labor in the control animals was similar, but it occurred at term rather than before term. The potential of this method in improving the management of medically indicated induction of preterm labor is discussed."}
{"id": "PMID:463989", "title": "Six years' experience with screening of a diethylstilbestrol-exposed population.", "content": "The physician population delivering obstetric care in Philadelphia between 1950 and 1970 was contacted to ascertain their use of diethylstilbesterol (DES) during pregnancy. Of the 31.8% of the physicians who responded to the questionnaire, 71.8% used DES during pregnancy and 12.7% desired assistance in review of their records. During the 6 years from the initial survey, 830 young women exposed to DES in utero were periodically screened for cervicovaginal abnormalities and clear cell adenocarcinoma. Of these 830 patients 61.7% were found to have cervicovaginal abnormalities, and 65.9% of the patients showed either adenosis or evidence of the prior existence of vaginal adenosis. Eight patients were treated for clear cell adenocarcinoma. Two cases were detected while asymptomatic. Seven of the patients are living with no evidence of cancer, and two of these have survived over 5 years.", "contents": "Six years' experience with screening of a diethylstilbestrol-exposed population. The physician population delivering obstetric care in Philadelphia between 1950 and 1970 was contacted to ascertain their use of diethylstilbesterol (DES) during pregnancy. Of the 31.8% of the physicians who responded to the questionnaire, 71.8% used DES during pregnancy and 12.7% desired assistance in review of their records. During the 6 years from the initial survey, 830 young women exposed to DES in utero were periodically screened for cervicovaginal abnormalities and clear cell adenocarcinoma. Of these 830 patients 61.7% were found to have cervicovaginal abnormalities, and 65.9% of the patients showed either adenosis or evidence of the prior existence of vaginal adenosis. Eight patients were treated for clear cell adenocarcinoma. Two cases were detected while asymptomatic. Seven of the patients are living with no evidence of cancer, and two of these have survived over 5 years."}
{"id": "PMID:463990", "title": "Quality of life for the gynecologic oncology patient.", "content": "An attempt is made to define the quality of life in terms of the patient with gynecologic cancer. The effect of the diagnosis of cancer and its recurrence or its terminal state on the quality of life is considered. Possible methods of assessing the patient's individual feelings about the quality of her life are reviewed and suggestions made for incorporating these into plans of management at all stages of the disease.", "contents": "Quality of life for the gynecologic oncology patient. An attempt is made to define the quality of life in terms of the patient with gynecologic cancer. The effect of the diagnosis of cancer and its recurrence or its terminal state on the quality of life is considered. Possible methods of assessing the patient's individual feelings about the quality of her life are reviewed and suggestions made for incorporating these into plans of management at all stages of the disease."}
{"id": "PMID:463991", "title": "Ectopic pregnancy: current clinical trends.", "content": "During the 16 year period ending in November, 1978, 191 cases of ectopic pregnancy were managed at the University of Virginia Hospital. The overall incidence was 1/126 deliveries but during the last 3 years of the study the incidence was 1/60 deliveries. Only 56 patients have had a subsequent conception. Thirteen have had a recurrent ectopic implantation. Only 36 women (23.7% of those available for follow-up) have had subsequent term pregnancies. Ectopic pregnancy continues to be a major gynecologic problem and the potential for subsequent fertility is poor.", "contents": "Ectopic pregnancy: current clinical trends. During the 16 year period ending in November, 1978, 191 cases of ectopic pregnancy were managed at the University of Virginia Hospital. The overall incidence was 1/126 deliveries but during the last 3 years of the study the incidence was 1/60 deliveries. Only 56 patients have had a subsequent conception. Thirteen have had a recurrent ectopic implantation. Only 36 women (23.7% of those available for follow-up) have had subsequent term pregnancies. Ectopic pregnancy continues to be a major gynecologic problem and the potential for subsequent fertility is poor."}
{"id": "PMID:463992", "title": "Bicornuate uterus and partial atresia of the fallopian tube.", "content": "Partial atresia of the fallopian tube was discovered in two patients with double uteri of the bicornuate variety. The abnormal tube in each case was ipsilateral to a noncommunicating, nonfunctioning, rudimentary uterine horn. A normal fimbriated end led to a short, patent ampullary portion that ended blindly in the mesosalpinx. Realization of the potential for the coexistence of congenital malformation of the fallopian tubes with various uterine malformations suggests that extreme caution be exercised during adnexal surgery in patients with anomalous development of the m\u00fcllerian ducts.", "contents": "Bicornuate uterus and partial atresia of the fallopian tube. Partial atresia of the fallopian tube was discovered in two patients with double uteri of the bicornuate variety. The abnormal tube in each case was ipsilateral to a noncommunicating, nonfunctioning, rudimentary uterine horn. A normal fimbriated end led to a short, patent ampullary portion that ended blindly in the mesosalpinx. Realization of the potential for the coexistence of congenital malformation of the fallopian tubes with various uterine malformations suggests that extreme caution be exercised during adnexal surgery in patients with anomalous development of the m\u00fcllerian ducts."}
{"id": "PMID:463993", "title": "Tubal ligation by colpotomy incision.", "content": "A five-year review of colpotomy tubal ligation performed on 585 patients within a private-practice setting in Columbus, Georgia, is analyzed. Operative time averaged under 30 minutes, and average hospital stay was less than 3 days. The major postoperative complication rate was less than 2%. The patient population is reviewed as to age, parity, previous contraception, and medical indications for sterilization. Surgical technique is discussed, and several suggestions are made, A follow-up of subsequent gynecologic procedures and the interval following colpotomy is then presented.", "contents": "Tubal ligation by colpotomy incision. A five-year review of colpotomy tubal ligation performed on 585 patients within a private-practice setting in Columbus, Georgia, is analyzed. Operative time averaged under 30 minutes, and average hospital stay was less than 3 days. The major postoperative complication rate was less than 2%. The patient population is reviewed as to age, parity, previous contraception, and medical indications for sterilization. Surgical technique is discussed, and several suggestions are made, A follow-up of subsequent gynecologic procedures and the interval following colpotomy is then presented."}
{"id": "PMID:463994", "title": "Radical hysterectomy: a critical review of twenty-two years' experience.", "content": "One hundred seventy-eight patients who had radical hysterectomy performed for management of invasive carcinoma of the cervix over 22 year period at the Medical University of South Carolina were critically analyzed. The degree of differentiation of the malignancy appeared to be of more significance than tumor size in Stage 1 lesions. With proper patient selection and excellent operative technique, survival rates well over 90% with minimal complications can be obtained. The radical hysterectomy should never be used in association with irradiation.", "contents": "Radical hysterectomy: a critical review of twenty-two years' experience. One hundred seventy-eight patients who had radical hysterectomy performed for management of invasive carcinoma of the cervix over 22 year period at the Medical University of South Carolina were critically analyzed. The degree of differentiation of the malignancy appeared to be of more significance than tumor size in Stage 1 lesions. With proper patient selection and excellent operative technique, survival rates well over 90% with minimal complications can be obtained. The radical hysterectomy should never be used in association with irradiation."}
{"id": "PMID:463995", "title": "Maternal transport: behind the drama.", "content": "Despite a lack of proof that overall perinatal outcome is improved, physicians and patients participate in a growing regional maternal transport program in a community hospital setting. Over the course of 3 1/2 years, 129 patients were transferred from 23 institutions. Maternal transport and the changes which result in the interactions of the physicians are compared to traditional referral systems. Obstacles and guidelines for minimizing them are discussed.", "contents": "Maternal transport: behind the drama. Despite a lack of proof that overall perinatal outcome is improved, physicians and patients participate in a growing regional maternal transport program in a community hospital setting. Over the course of 3 1/2 years, 129 patients were transferred from 23 institutions. Maternal transport and the changes which result in the interactions of the physicians are compared to traditional referral systems. Obstacles and guidelines for minimizing them are discussed."}
{"id": "PMID:463996", "title": "Hypothalamic-pituitary function and radiographic evaluation of women with hyperprolactinemia and an \"empty\" sella turcica.", "content": "A primary \"empty\" sella may be associated with significant hypothalamic-pituitary dysfunction. This report characterizes the endocrinologic and radiographic findings in six patients with hyperprolactinemia (range 34.3 to 1,170 ng/ml) ultimately found to have an enlarged empty sella. Lateral skull x-ray films and thin-section tomograms were suggestive of pituitary tumors in all patients. Four patients underwent transphenoidal sellar exploration after pneumoencephalography (PEG) failed to demonstrate air within the sella. The inability of PEG to demonstrate an empty sella in two patients was explainable on the basis of an intact diaphragma sellae with previous transient intrasellar pathology responsible for the sellar enlargement. Dynamic hypothalamic-pituitary testing yielded no consistent pattern of response. These studies suggest that an empty sella: (1) may be associated with hyperprolactinemia, regardless of etiology, (2) is not diagnosable by dynamic hormonal testing, and (3) may be indistinguishable from a pituitary tumor by current radiographic techniques.", "contents": "Hypothalamic-pituitary function and radiographic evaluation of women with hyperprolactinemia and an \"empty\" sella turcica. A primary \"empty\" sella may be associated with significant hypothalamic-pituitary dysfunction. This report characterizes the endocrinologic and radiographic findings in six patients with hyperprolactinemia (range 34.3 to 1,170 ng/ml) ultimately found to have an enlarged empty sella. Lateral skull x-ray films and thin-section tomograms were suggestive of pituitary tumors in all patients. Four patients underwent transphenoidal sellar exploration after pneumoencephalography (PEG) failed to demonstrate air within the sella. The inability of PEG to demonstrate an empty sella in two patients was explainable on the basis of an intact diaphragma sellae with previous transient intrasellar pathology responsible for the sellar enlargement. Dynamic hypothalamic-pituitary testing yielded no consistent pattern of response. These studies suggest that an empty sella: (1) may be associated with hyperprolactinemia, regardless of etiology, (2) is not diagnosable by dynamic hormonal testing, and (3) may be indistinguishable from a pituitary tumor by current radiographic techniques."}
{"id": "PMID:463997", "title": "Identification of differing etiologies of clinically diagnosed premature menopause.", "content": "Investigations were performed in eight young women to determine if the findings of secondary amenorrhea and high follicle-stimulating hormone levels were due to primary ovarian follicular atresia or to other causes. Karyotypes were determined from both peripheral leukocytes and ovarian tissue; one woman had XXX/XX/XO mosaicism. Another woman had normal ovarian histology and probably had the \"gonadotropin-resistant ovary syndrome.\" No autoimmune antibodies were detected, but one woman with myasthenia gravis also had ovarian histology that demonstrated primary ova and a developing follicle. Only five of eight women had primary ovarian follicular atresia, and two of the other three women had conditions theoretically compatible with subsequent pregnancy.", "contents": "Identification of differing etiologies of clinically diagnosed premature menopause. Investigations were performed in eight young women to determine if the findings of secondary amenorrhea and high follicle-stimulating hormone levels were due to primary ovarian follicular atresia or to other causes. Karyotypes were determined from both peripheral leukocytes and ovarian tissue; one woman had XXX/XX/XO mosaicism. Another woman had normal ovarian histology and probably had the \"gonadotropin-resistant ovary syndrome.\" No autoimmune antibodies were detected, but one woman with myasthenia gravis also had ovarian histology that demonstrated primary ova and a developing follicle. Only five of eight women had primary ovarian follicular atresia, and two of the other three women had conditions theoretically compatible with subsequent pregnancy."}
{"id": "PMID:463998", "title": "Long-term course of chloroquine retinopathy after cessation of medication.", "content": "Seven patients with chloroquine retinopathy were examined ten years after their therapy with chloroquine or hydroxychloroquine, or both, had been discontinued and an additional five patients with chloroquine retinopathy were similarly examined from two to eight years after their therapy had been discontinued. Visual acuity, visual fields, and ophthalmoscopic examinations were compared to those performed at the time therapy was discontinued. These long-term observations confirmed the previously published observations based on short-term studies that chloroquine retinopathy tends to remain stable after therapy is discontinued, although a few patients in the early stages of retinopathy may show regression and occasionally a patient with a more advanced stage of the disease may show progression.", "contents": "Long-term course of chloroquine retinopathy after cessation of medication. Seven patients with chloroquine retinopathy were examined ten years after their therapy with chloroquine or hydroxychloroquine, or both, had been discontinued and an additional five patients with chloroquine retinopathy were similarly examined from two to eight years after their therapy had been discontinued. Visual acuity, visual fields, and ophthalmoscopic examinations were compared to those performed at the time therapy was discontinued. These long-term observations confirmed the previously published observations based on short-term studies that chloroquine retinopathy tends to remain stable after therapy is discontinued, although a few patients in the early stages of retinopathy may show regression and occasionally a patient with a more advanced stage of the disease may show progression."}
{"id": "PMID:463999", "title": "Aciclovir and corneal wound healing.", "content": "Masked controlled rabbit studies were done to determine the toxic effects on corneal wound healing of the antiviral drugs 3% aciclovir and 0.5% idoxuridine ointment in therapeutically effective concentrations. Aciclovir had no significant detrimental effect in comparison to controls on the quality of regenerating epithelium or the re-epithelialization of epithelial wounds. Idoxuridine treatment caused significant toxic changes in the regenerating epithelium clinically and histologically with a significant delay in epithelial wound healing in comparison to control or aciclovir treated eyes. Aciclovir had no significant effect on the collagen content of stromal wounds as measured by hydroxyproline levels. Idoxuridine caused a reduction in collagen content not significantly different from controls but significantly lower than aciclovir.", "contents": "Aciclovir and corneal wound healing. Masked controlled rabbit studies were done to determine the toxic effects on corneal wound healing of the antiviral drugs 3% aciclovir and 0.5% idoxuridine ointment in therapeutically effective concentrations. Aciclovir had no significant detrimental effect in comparison to controls on the quality of regenerating epithelium or the re-epithelialization of epithelial wounds. Idoxuridine treatment caused significant toxic changes in the regenerating epithelium clinically and histologically with a significant delay in epithelial wound healing in comparison to control or aciclovir treated eyes. Aciclovir had no significant effect on the collagen content of stromal wounds as measured by hydroxyproline levels. Idoxuridine caused a reduction in collagen content not significantly different from controls but significantly lower than aciclovir."}
{"id": "PMID:464000", "title": "Apneic spells associated with timolol therapy in a neonate.", "content": "A 2-week-old premature child with congenital glaucoma secondary to anterior cleavage syndrome was treated with timolol maleate and cyclocryotherapy. The patient had apneic spells of up to 30 seconds that stopped soon after timolol maleate therapy was discontinued. No apnea was seen before timolol maleate administration, and no further spells were noted after subsequent cyclocryotherapy without timolol maleate treatment. Possible central nervous system toxicity of timol maleate or its metabolic by-products in neonates with immature blood-brain barriers was noted.", "contents": "Apneic spells associated with timolol therapy in a neonate. A 2-week-old premature child with congenital glaucoma secondary to anterior cleavage syndrome was treated with timolol maleate and cyclocryotherapy. The patient had apneic spells of up to 30 seconds that stopped soon after timolol maleate therapy was discontinued. No apnea was seen before timolol maleate administration, and no further spells were noted after subsequent cyclocryotherapy without timolol maleate treatment. Possible central nervous system toxicity of timol maleate or its metabolic by-products in neonates with immature blood-brain barriers was noted."}
{"id": "PMID:464001", "title": "Massive subretinal exudate after retinal detachment surgery.", "content": "A 70-year-old woman developed massive subretinal exudation after surgical repair of a rhegmatogenous retinal detachment. Postoperative choroidal detachment suggested a choroidal source of the subretinal exudate. We believe the cause of the choroidal detachment and exudation was vortex ampulla obstruction resulting from the encircling band. The subretinal exudate underwent gradual resolution and did not influence the anatomic result of retinal reattachment.", "contents": "Massive subretinal exudate after retinal detachment surgery. A 70-year-old woman developed massive subretinal exudation after surgical repair of a rhegmatogenous retinal detachment. Postoperative choroidal detachment suggested a choroidal source of the subretinal exudate. We believe the cause of the choroidal detachment and exudation was vortex ampulla obstruction resulting from the encircling band. The subretinal exudate underwent gradual resolution and did not influence the anatomic result of retinal reattachment."}
{"id": "PMID:464002", "title": "A wireform surgical drape-retractor.", "content": "I devised a malleable plastic-coated wireform drape-retractor that increases patient comfort during surgery by keeping drapes off the nose and mouth. It is small and does not interfere with the surgeon's hand movements.", "contents": "A wireform surgical drape-retractor. I devised a malleable plastic-coated wireform drape-retractor that increases patient comfort during surgery by keeping drapes off the nose and mouth. It is small and does not interfere with the surgeon's hand movements."}
{"id": "PMID:464003", "title": "A retention modification for the limbal ring method of foreign body localization.", "content": "For intraocular foreign body localization, we used a modification of the limbal ring method with surgical adhesive instead of limbal sutures. After taking x-ray films of the eyeball, we removed the ring and the adherent glue with toothless forceps.", "contents": "A retention modification for the limbal ring method of foreign body localization. For intraocular foreign body localization, we used a modification of the limbal ring method with surgical adhesive instead of limbal sutures. After taking x-ray films of the eyeball, we removed the ring and the adherent glue with toothless forceps."}
{"id": "PMID:464009", "title": "Traumatic retinal pigment epithelial edema.", "content": "A 17-year-old girl was struct in the right eye by a mortarboard tossed into the air during high school graduation exercises. She suffered commotio retinae with damage to the retinal pigment epithelium and macular hole formation. Fluorescein angiography in the acute stages revealed diffuse staining at the level of the pigment epithelium. Five months later, the areas of abnormal staining had undergone depigmentation and dye no longer accumulated in the pigment epithelial layer. The fluorescein findings in traumatic retinal pigment epithelial edema are unusual.", "contents": "Traumatic retinal pigment epithelial edema. A 17-year-old girl was struct in the right eye by a mortarboard tossed into the air during high school graduation exercises. She suffered commotio retinae with damage to the retinal pigment epithelium and macular hole formation. Fluorescein angiography in the acute stages revealed diffuse staining at the level of the pigment epithelium. Five months later, the areas of abnormal staining had undergone depigmentation and dye no longer accumulated in the pigment epithelial layer. The fluorescein findings in traumatic retinal pigment epithelial edema are unusual."}
{"id": "PMID:464010", "title": "Cystoid macular edema as the primary sign of neurosyphilis.", "content": "A 34-year-old man had a six-month history of bilateral visual loss that was secondary to cystoid macular edema, which was assumed to be secondary to neurosyphilis on the basis of cerebrospinal fluid serology, cell count, and protein. Good visual acuity was recovered with systemic corticosteroids only after they were used in combination with antitreponemal therapy. This is the first report, to the best of our knowledge, of cystoid macular edema as the primary sign of neurosyphilis documented by fluorescein angiography.", "contents": "Cystoid macular edema as the primary sign of neurosyphilis. A 34-year-old man had a six-month history of bilateral visual loss that was secondary to cystoid macular edema, which was assumed to be secondary to neurosyphilis on the basis of cerebrospinal fluid serology, cell count, and protein. Good visual acuity was recovered with systemic corticosteroids only after they were used in combination with antitreponemal therapy. This is the first report, to the best of our knowledge, of cystoid macular edema as the primary sign of neurosyphilis documented by fluorescein angiography."}
{"id": "PMID:464011", "title": "Retinal astrocytoma.", "content": "A 41-year-old man had a 6 x 6 x 5-disk diameter amelanotic tumor in the posterior fundus. The clinical and fluorescein angiographic appearance suggested a benign retinal vascular tumor, although amelanotic choroidal melanoma and retinoblastoma were diagnostic possibilities. An incisional 48-hour radioactive phosphorus (32P) uptake test was performed and the result showed an increased uptake over the tumor mass of 100% as compared to the control quadrants. The globe was enucleated and the pathologic diagnosis was isolated astrocytic glioma of the retina with minimal, if any, malignant potential. The highly developed vascular system of the tumor probably contributed to the false-positive test result.", "contents": "Retinal astrocytoma. A 41-year-old man had a 6 x 6 x 5-disk diameter amelanotic tumor in the posterior fundus. The clinical and fluorescein angiographic appearance suggested a benign retinal vascular tumor, although amelanotic choroidal melanoma and retinoblastoma were diagnostic possibilities. An incisional 48-hour radioactive phosphorus (32P) uptake test was performed and the result showed an increased uptake over the tumor mass of 100% as compared to the control quadrants. The globe was enucleated and the pathologic diagnosis was isolated astrocytic glioma of the retina with minimal, if any, malignant potential. The highly developed vascular system of the tumor probably contributed to the false-positive test result."}
{"id": "PMID:464012", "title": "Adenoma of the pigment epithelium of the ciliary body simulating a malignant melanoma.", "content": "A benign adenoma of the pigment epithelium of the ciliary body in a 27-year-old woman simulated a malignant melanoma clinically and resulted in enucleation. The correct diagnosis was not made clinically despite the use of A- and B- scan ultrasonography and the radioactive phosphorus (32P) uptake test, procedures which are usually reliable in differentiating between melanoma and pseudomelanoma. We emphasize the shortcomings of these ancillary tests in differentiating pigment epithelial tumors from malignant melanoma.", "contents": "Adenoma of the pigment epithelium of the ciliary body simulating a malignant melanoma. A benign adenoma of the pigment epithelium of the ciliary body in a 27-year-old woman simulated a malignant melanoma clinically and resulted in enucleation. The correct diagnosis was not made clinically despite the use of A- and B- scan ultrasonography and the radioactive phosphorus (32P) uptake test, procedures which are usually reliable in differentiating between melanoma and pseudomelanoma. We emphasize the shortcomings of these ancillary tests in differentiating pigment epithelial tumors from malignant melanoma."}
{"id": "PMID:464013", "title": "Cystoid macular edema in aphakic and pseudophakic eyes.", "content": "A prospective fluorescein angiographic study, comparing the incidence of cystoid macular edema in patients 67 years or older after intracapsular cataract extraction with a Binkhorst 4-loop implant, extracapsular cataract extraction with a Binkhorst 2-loop or 4-loop implant, and intracapsular cataract extraction with no implant (controls) was begun at the Bascom Palmer Eye Institute of the University of Miami School of Medicine on May 14, 1974. Excluded from these groups were all patients whom we did not consider to be suitable candidates for lens implant surgery. Fundus fluorescein angiograms were taken four, eight, and 16 to 24 months postoperatively. The surgery was performed by one group of surgeons and the angiograms were read by a group of retinal specialists. The intracapsular cataract extraction-implant and intracapsular-no implant groups had a comparable incidence of cystoid macular edema four, eight, and 16 to 24 months postoperatively. The extra capsular cataract extraction-implant and an intact posterior capsule group had a significantly lower incidence of cystoid macular edema than the intracapsular cataract extraction-implant group 16 to 24 months postoperatively. The differences were less significant at four and eight months. Visual function as measured by visual acuity was 6/12 (20/40) or better in approximately 80% of eyes with angiographic-proven cystoid macular edema.", "contents": "Cystoid macular edema in aphakic and pseudophakic eyes. A prospective fluorescein angiographic study, comparing the incidence of cystoid macular edema in patients 67 years or older after intracapsular cataract extraction with a Binkhorst 4-loop implant, extracapsular cataract extraction with a Binkhorst 2-loop or 4-loop implant, and intracapsular cataract extraction with no implant (controls) was begun at the Bascom Palmer Eye Institute of the University of Miami School of Medicine on May 14, 1974. Excluded from these groups were all patients whom we did not consider to be suitable candidates for lens implant surgery. Fundus fluorescein angiograms were taken four, eight, and 16 to 24 months postoperatively. The surgery was performed by one group of surgeons and the angiograms were read by a group of retinal specialists. The intracapsular cataract extraction-implant and intracapsular-no implant groups had a comparable incidence of cystoid macular edema four, eight, and 16 to 24 months postoperatively. The extra capsular cataract extraction-implant and an intact posterior capsule group had a significantly lower incidence of cystoid macular edema than the intracapsular cataract extraction-implant group 16 to 24 months postoperatively. The differences were less significant at four and eight months. Visual function as measured by visual acuity was 6/12 (20/40) or better in approximately 80% of eyes with angiographic-proven cystoid macular edema."}
{"id": "PMID:464014", "title": "Retinal detachment in pseudophakia.", "content": "In a series of cataract patients excluding myopic individuals, under age 60 years, and cases in which vitreous loss occurred, retinal detachment was no less frequent after intracapsular cataract extraction and Sputnik iris supported lenses than in controls. Both groups were followed up for a minimum of two years. The detachments predominantly occurred from retinal breaks in areas of the retina that looked normal preoperatively.", "contents": "Retinal detachment in pseudophakia. In a series of cataract patients excluding myopic individuals, under age 60 years, and cases in which vitreous loss occurred, retinal detachment was no less frequent after intracapsular cataract extraction and Sputnik iris supported lenses than in controls. Both groups were followed up for a minimum of two years. The detachments predominantly occurred from retinal breaks in areas of the retina that looked normal preoperatively."}
{"id": "PMID:464015", "title": "Time analysis of corneal endothelial cell density after cataract extraction.", "content": "Serial endothelial photographs were taken preoperatively and postoperatively in 200 eyes; 111 eyes contained a Rayner iris clip lens, 54 eyes contained a Fyodorov Sputnik lens, and 35 eyes had no lens. Central endothelial cell density was changed in all instances, with counts in implanted eyes declining 25 to 30%, and in nonimplanted eyes 10 to 15%. In both instances, the decline essentially ceased at about three months. The cause of the greater decline in implanted eyes appeared to be mechanical and subsequent cell loss after the 90-day period was virtually equal for the two groups. Methods that may be used to alter the difference in cell density occurring with implantation are best analyzed by using the 90-day period data for comparison.", "contents": "Time analysis of corneal endothelial cell density after cataract extraction. Serial endothelial photographs were taken preoperatively and postoperatively in 200 eyes; 111 eyes contained a Rayner iris clip lens, 54 eyes contained a Fyodorov Sputnik lens, and 35 eyes had no lens. Central endothelial cell density was changed in all instances, with counts in implanted eyes declining 25 to 30%, and in nonimplanted eyes 10 to 15%. In both instances, the decline essentially ceased at about three months. The cause of the greater decline in implanted eyes appeared to be mechanical and subsequent cell loss after the 90-day period was virtually equal for the two groups. Methods that may be used to alter the difference in cell density occurring with implantation are best analyzed by using the 90-day period data for comparison."}
{"id": "PMID:464017", "title": "Culture of human glomerular cells.", "content": "Human glomeruli were routinely cultured in Waymouth's medium supplemented with insulin and conditioned medium. Three cell types were seen in the culture of both adult and infant kidneys, but the morphology of the glomerular cellular outgrowths depended on the age of the patient from which the kidney was obtained. Cultures of glomeruli from older individuals resulted in more \"differentiated\" cells, but both adult and infant glomerular cells rapidly became \"dedifferentiated\" as the length of time in culture increased. Outgrowths of cultured glomeruli did not contain fibroblasts or tubular cells. Finally, synthesis of basement membrane material by these cultured glomerular cells was demonstrated.", "contents": "Culture of human glomerular cells. Human glomeruli were routinely cultured in Waymouth's medium supplemented with insulin and conditioned medium. Three cell types were seen in the culture of both adult and infant kidneys, but the morphology of the glomerular cellular outgrowths depended on the age of the patient from which the kidney was obtained. Cultures of glomeruli from older individuals resulted in more \"differentiated\" cells, but both adult and infant glomerular cells rapidly became \"dedifferentiated\" as the length of time in culture increased. Outgrowths of cultured glomeruli did not contain fibroblasts or tubular cells. Finally, synthesis of basement membrane material by these cultured glomerular cells was demonstrated."}
{"id": "PMID:464018", "title": "Correlation of estrogen and progesterone receptors with histologic differentiation in endometrial adenocarcinoma.", "content": "Endometrial carcinomas from 58 patients were graded histologically, and the histologic grade was compared with the estrogen and progesterone receptor content of the tumor tissues. Receptor content was determined by multiple concentration saturation analysis and sucrose density gradient analysis. A positive correlation was found between the presence of estrogen and progesterone receptors and the degree of tumor differentiation. The majority (85%) of well-differentiated lesions contained significant amounts of both steroid receptor proteins. In contrast, only 13% of the poorly differentiated lesions were characterized by the presence of detectable levels of both estrogen and progesterone receptor proteins. The relationship of receptor content and degree of differentiation to prognosis and potential for response to hormonal therapy is discussed.", "contents": "Correlation of estrogen and progesterone receptors with histologic differentiation in endometrial adenocarcinoma. Endometrial carcinomas from 58 patients were graded histologically, and the histologic grade was compared with the estrogen and progesterone receptor content of the tumor tissues. Receptor content was determined by multiple concentration saturation analysis and sucrose density gradient analysis. A positive correlation was found between the presence of estrogen and progesterone receptors and the degree of tumor differentiation. The majority (85%) of well-differentiated lesions contained significant amounts of both steroid receptor proteins. In contrast, only 13% of the poorly differentiated lesions were characterized by the presence of detectable levels of both estrogen and progesterone receptor proteins. The relationship of receptor content and degree of differentiation to prognosis and potential for response to hormonal therapy is discussed."}
{"id": "PMID:464019", "title": "Gene complex controlling growth and fertility linked to the major histocompatibility complex in the rat.", "content": "The B1 strain of rats carries a unique mutation which causes defects in growth and reproduction: the males and females are small, the testes are hypoplastic and aspermatic, and the females have a reduced reproductive capacity. The loci controlling these defects are linked to the major histocompatibility complex (MHC) as determined by segregation studies in backcross and F2 hybrid populations. The levels of pituitary hormones and somatomedin C in the B1 strain are elevated or normal, and the testosterone level is elevated relative to the size of the testes. These findings suggest that hormone deficiencies are not the cause of these defects. The genes governing these defects have been designated the growth and reproduction complex (Grc). The recessive gene regulating small body size has been designated dw-3 (dwarf-3), and the recessive gene influencing reproductive capacity has been designated f. The Grc and MHC are separable by recombination, and the dw-3 and f genes are also separable by recombination. Studies in the (B1 X DA)F2 hybrid indicate that the map distance between the Grc and the MHC is 0.6 cM. Segregation distortion due to a deficiency of RT11 homozygotes is seen in some F2 hybrid populations derived from the B1 strain. Litter size data suggest that the loss of the RT11 homozygotes is due to intrauterine death. There is no apparent sex influence on the inheritance of the Grc, at least as it is presently understood, since it can be transmitted by either females or males. The growth and reproduction complex in the rat may be the analog of the T/t complex in the mouse, and the importance of the region of the chromosome adjacent to the major histocompatibility complex in the control of developmental processes may be a general phenomenon in mammals.", "contents": "Gene complex controlling growth and fertility linked to the major histocompatibility complex in the rat. The B1 strain of rats carries a unique mutation which causes defects in growth and reproduction: the males and females are small, the testes are hypoplastic and aspermatic, and the females have a reduced reproductive capacity. The loci controlling these defects are linked to the major histocompatibility complex (MHC) as determined by segregation studies in backcross and F2 hybrid populations. The levels of pituitary hormones and somatomedin C in the B1 strain are elevated or normal, and the testosterone level is elevated relative to the size of the testes. These findings suggest that hormone deficiencies are not the cause of these defects. The genes governing these defects have been designated the growth and reproduction complex (Grc). The recessive gene regulating small body size has been designated dw-3 (dwarf-3), and the recessive gene influencing reproductive capacity has been designated f. The Grc and MHC are separable by recombination, and the dw-3 and f genes are also separable by recombination. Studies in the (B1 X DA)F2 hybrid indicate that the map distance between the Grc and the MHC is 0.6 cM. Segregation distortion due to a deficiency of RT11 homozygotes is seen in some F2 hybrid populations derived from the B1 strain. Litter size data suggest that the loss of the RT11 homozygotes is due to intrauterine death. There is no apparent sex influence on the inheritance of the Grc, at least as it is presently understood, since it can be transmitted by either females or males. The growth and reproduction complex in the rat may be the analog of the T/t complex in the mouse, and the importance of the region of the chromosome adjacent to the major histocompatibility complex in the control of developmental processes may be a general phenomenon in mammals."}
{"id": "PMID:464020", "title": "The structure of tight junctions in human thyroid tumors. A systematic freeze-fracture study.", "content": "Tight junctions of normal human epithelium comprise 6--8 intramembranous ridges. Tight junctions of adenomas and one case of follicular carcinoma were morphologically identical with normal controls. Another follicular carcinoma (clinically less malignant than the case mentioned above), however, showed loss of junctional polarity with spreading of ridges on lateral cell membranes. One case of papillary carcinoma (clinically low malignancy) displayed only focal attenuations of the tight junction belt. Another more malignant tumor of the same histologic type showed breaks of ridges and focal interruptions of the tight junction meshwork. Cell membranes of two anaplastic carcinomas of high malignancy were completely devoid of tight junction ridges. We conclude that tight junction alterations are not necessarily implicated in malignant transformation and that they correspond with tumor differentiation rather than directly with a single parameter of thyroid tumor malignancy.", "contents": "The structure of tight junctions in human thyroid tumors. A systematic freeze-fracture study. Tight junctions of normal human epithelium comprise 6--8 intramembranous ridges. Tight junctions of adenomas and one case of follicular carcinoma were morphologically identical with normal controls. Another follicular carcinoma (clinically less malignant than the case mentioned above), however, showed loss of junctional polarity with spreading of ridges on lateral cell membranes. One case of papillary carcinoma (clinically low malignancy) displayed only focal attenuations of the tight junction belt. Another more malignant tumor of the same histologic type showed breaks of ridges and focal interruptions of the tight junction meshwork. Cell membranes of two anaplastic carcinomas of high malignancy were completely devoid of tight junction ridges. We conclude that tight junction alterations are not necessarily implicated in malignant transformation and that they correspond with tumor differentiation rather than directly with a single parameter of thyroid tumor malignancy."}
{"id": "PMID:464021", "title": "Studies of the Pelger-Hu\u00ebt anomaly in foxhounds.", "content": "An inbred family of foxhounds with four members expressing the Pelger-Hu\u00ebt (P-H) anomaly is described. The disease-free status of all P-H affected dogs suggests a benign disorder, although review of breeding records indicated a lower percentage of pups weaned (63%) by P-H females compared with the percentage of pups weaned (81%) by outcrossed females throughout the foxhound colony. Light-microscopic examination of blood films from affected dogs revealed 50--67% neutrophils with round, oval, or bean-shaped nuclei and rarely (0.5%) segmented nuclei. Neutrophils examined by electron microscopy showed the nuclei to have a fine nuclear cleft and condensed chromatin and the cytoplasm to have mature heterochromatic granulation. Local P-H neutrophil mobilization through a standard skin abrasion into a chamber containing autologous serum was impaired at all time periods evaluated (1, 4, 8, and 24 hours) compared with the neutrophil mobilization by normal dogs. Antibody response to sheep erythrocyte immunization was also impaired. In vitro reactivity of normal and P-H lymphocytes stimulated by pokeweed mitogen was depressed when lymphocytes were cultured in plasma from a P-H dog but not when cultured in plasma from a normal dog. Vigorous blastogenic responses to phytohemagglutinin by normal and P-H lymphocytes cultured in P-H or normal plasma suggest the presence of a factor(s) in the P-H plasma which interferes with B-lymphocyte reactivity.", "contents": "Studies of the Pelger-Hu\u00ebt anomaly in foxhounds. An inbred family of foxhounds with four members expressing the Pelger-Hu\u00ebt (P-H) anomaly is described. The disease-free status of all P-H affected dogs suggests a benign disorder, although review of breeding records indicated a lower percentage of pups weaned (63%) by P-H females compared with the percentage of pups weaned (81%) by outcrossed females throughout the foxhound colony. Light-microscopic examination of blood films from affected dogs revealed 50--67% neutrophils with round, oval, or bean-shaped nuclei and rarely (0.5%) segmented nuclei. Neutrophils examined by electron microscopy showed the nuclei to have a fine nuclear cleft and condensed chromatin and the cytoplasm to have mature heterochromatic granulation. Local P-H neutrophil mobilization through a standard skin abrasion into a chamber containing autologous serum was impaired at all time periods evaluated (1, 4, 8, and 24 hours) compared with the neutrophil mobilization by normal dogs. Antibody response to sheep erythrocyte immunization was also impaired. In vitro reactivity of normal and P-H lymphocytes stimulated by pokeweed mitogen was depressed when lymphocytes were cultured in plasma from a P-H dog but not when cultured in plasma from a normal dog. Vigorous blastogenic responses to phytohemagglutinin by normal and P-H lymphocytes cultured in P-H or normal plasma suggest the presence of a factor(s) in the P-H plasma which interferes with B-lymphocyte reactivity."}
{"id": "PMID:464022", "title": "Induction of IgE antibodies to antigen isolated from tobacco leaves and from cigarette smoke condensate.", "content": "Neonatal California White rabbits were sensitized with a glycoprotein purified from cured Virginia Bright tobacco leaves. Their serums, but not serums from normal rabbits, were demonstrated by passive cutaneous anaphylaxis technique to contain heat-labile, homocytophilic antibodies to this antigen and to similar material purified from cigarette smoke condensate. Serums containing IgE antibodies to tobacco antigen would not be demonstrated to contain hemagglutinating antibodies to this antigen. These experiments demonstrate that antigen capable of triggering specific IgE-mediated release of inflammatory mediators is present in cigarette smoke. It can be hypothesized that IgE-mediated responses to antigen in cigarette smoke are causally related to the development of vascular injury and of myocardial arrhythmia in hypersensitive smokers.", "contents": "Induction of IgE antibodies to antigen isolated from tobacco leaves and from cigarette smoke condensate. Neonatal California White rabbits were sensitized with a glycoprotein purified from cured Virginia Bright tobacco leaves. Their serums, but not serums from normal rabbits, were demonstrated by passive cutaneous anaphylaxis technique to contain heat-labile, homocytophilic antibodies to this antigen and to similar material purified from cigarette smoke condensate. Serums containing IgE antibodies to tobacco antigen would not be demonstrated to contain hemagglutinating antibodies to this antigen. These experiments demonstrate that antigen capable of triggering specific IgE-mediated release of inflammatory mediators is present in cigarette smoke. It can be hypothesized that IgE-mediated responses to antigen in cigarette smoke are causally related to the development of vascular injury and of myocardial arrhythmia in hypersensitive smokers."}
{"id": "PMID:464023", "title": "A phenomenologic description of clot lysis in dilute human plasma correlating structure and function of platelets.", "content": "Platelets in a dilute platelet-rich plasma clot formed by the addition of thrombin at 4 C do not lose their alpha-granules or develop platelet-fibrin interconnections. These clots do not retract or lyse. If after clot formation for 30 minutes the clots are transferred to 37 C, the alpha-granules then disappear from the platelet, extensive platelet-fibrin interconnections develop, and the clots retract and lyse. When the dilute plasma clots, however, are formed at 37 C, alpha-granules are quickly lost, few platelet-fibrin interconnections develop, and the clots do not retract or lyse. Deoxyglucose and antimycin, when added before thrombin at 4 C, prevent the loss of alpha-granules and development of platelet-fibrin interconnections after transfer of the clot to 37 C. These inhibited platelets did not retract or lyse. From the results reported here we conclude that the development of platelet-fibrin interconnections as well as the retraction and lysis of the clot occur in conjunction with the release of alpha-granules from the platelet. It may be necessary for this release to occur after the fibrin network is completed, since release of the alpha-granules in the 37 C clot before clot formation did not result in formation of many platelet-fibrin interconnections or in clot retraction or lysis.", "contents": "A phenomenologic description of clot lysis in dilute human plasma correlating structure and function of platelets. Platelets in a dilute platelet-rich plasma clot formed by the addition of thrombin at 4 C do not lose their alpha-granules or develop platelet-fibrin interconnections. These clots do not retract or lyse. If after clot formation for 30 minutes the clots are transferred to 37 C, the alpha-granules then disappear from the platelet, extensive platelet-fibrin interconnections develop, and the clots retract and lyse. When the dilute plasma clots, however, are formed at 37 C, alpha-granules are quickly lost, few platelet-fibrin interconnections develop, and the clots do not retract or lyse. Deoxyglucose and antimycin, when added before thrombin at 4 C, prevent the loss of alpha-granules and development of platelet-fibrin interconnections after transfer of the clot to 37 C. These inhibited platelets did not retract or lyse. From the results reported here we conclude that the development of platelet-fibrin interconnections as well as the retraction and lysis of the clot occur in conjunction with the release of alpha-granules from the platelet. It may be necessary for this release to occur after the fibrin network is completed, since release of the alpha-granules in the 37 C clot before clot formation did not result in formation of many platelet-fibrin interconnections or in clot retraction or lysis."}
{"id": "PMID:464026", "title": "Hypoxia and incorporation of 3H-thymidine by cells of the rat pulmonary arteries and alveolar wall.", "content": "In the pulmonary arterial circulation hypoxia produces increase in thickness of the medial muscle coat as well as of the adventitia; in addition muscle appears in smaller arteries than is normal and the number of small arteries that fill on Micropaque-gelatin injection is reduced. To assess the role of hyperplasia in these changes, the uptake of 3H-thymidine by the cells of the pulmonary arterial wall has been studied in rats exposed to hypobaric hypoxia (exposure to 380 torr) after 1, 3, 5, 7, 10, and 14 days. Using autoradiographs of 1-micron sections, the glutaraldehyde-distended intrapulmonary hilar muscular artery, the peripheral, intraacinar arteries less than 100 micron in external diameter, and the alveolar wall had different patterns of uptake. In the hilar pulmonary artery, after 24 hours of exposure, the labeling index for adventitial fibroblasts is increased eightfold over the control value, and for endothelial cells, threefold, while for medial smooth muscle cells, there is a gradual and small increase to Day 14. Newly muscularized intraacinar arteries are first apparent at Day 3, when they comprise 40% of the intraacinar arteries, increasing to 80% at Day 7. No decrease in density of arteries is found. Uptake of 3H-thymidine by new muscle cells is not apparent until Day 5 when labeling is maximum. The endothelial cells of the newly muscularized arteries show an increased labeling index only at Days 7 and 10. The veins and normally muscular arteries do not show these changes. In the alveolar walls, the concentration of labeled cells is significantly above the control value at Days 3, 5, and 7 and significantly below, at Day 14. At this level, the interstitial, epithelial, and endothelial cells contribute to the increase.", "contents": "Hypoxia and incorporation of 3H-thymidine by cells of the rat pulmonary arteries and alveolar wall. In the pulmonary arterial circulation hypoxia produces increase in thickness of the medial muscle coat as well as of the adventitia; in addition muscle appears in smaller arteries than is normal and the number of small arteries that fill on Micropaque-gelatin injection is reduced. To assess the role of hyperplasia in these changes, the uptake of 3H-thymidine by the cells of the pulmonary arterial wall has been studied in rats exposed to hypobaric hypoxia (exposure to 380 torr) after 1, 3, 5, 7, 10, and 14 days. Using autoradiographs of 1-micron sections, the glutaraldehyde-distended intrapulmonary hilar muscular artery, the peripheral, intraacinar arteries less than 100 micron in external diameter, and the alveolar wall had different patterns of uptake. In the hilar pulmonary artery, after 24 hours of exposure, the labeling index for adventitial fibroblasts is increased eightfold over the control value, and for endothelial cells, threefold, while for medial smooth muscle cells, there is a gradual and small increase to Day 14. Newly muscularized intraacinar arteries are first apparent at Day 3, when they comprise 40% of the intraacinar arteries, increasing to 80% at Day 7. No decrease in density of arteries is found. Uptake of 3H-thymidine by new muscle cells is not apparent until Day 5 when labeling is maximum. The endothelial cells of the newly muscularized arteries show an increased labeling index only at Days 7 and 10. The veins and normally muscular arteries do not show these changes. In the alveolar walls, the concentration of labeled cells is significantly above the control value at Days 3, 5, and 7 and significantly below, at Day 14. At this level, the interstitial, epithelial, and endothelial cells contribute to the increase."}
{"id": "PMID:464027", "title": "Acute inflammatory pulmonary reactions induced by chemotactic factors.", "content": "Acute inflammatory reactions have been produced in hamster lungs by the intrapulmonary instillation of preformed chemotactic mediators (C5fr and F-Met-Leu-Phe). By the use of 111Indium and 125Iodine labeling of homologous neutrophils (PMNs) and homologous albumin, respectively, it has been possible to obtain highly reproducible and quantitative parameters of the acute inflammatory response. The lung responds in a dose-dependent and time-dependent manner to the instillation of preformed chemotactic mediators. The quantitative parameters indicative of PMN influx were more prominent than changes in vascular permeability. The data obtained by the use of radiolabeled PMNs were confirmed by observation with light microscopy. Nonchemotactic substances such as human IgG, serum albumin, and C4 failed to induce inflammatory responses in lung. Interestingly, intact C5 instilled into lung was extremely phlogistic, apparently due to hydrolysis once within the lung. These studies provide an approach to reliable quantitative parameters of inflammatory reactions in the lung and emphasize the in vivo biologic effects of chemotactic mediators.", "contents": "Acute inflammatory pulmonary reactions induced by chemotactic factors. Acute inflammatory reactions have been produced in hamster lungs by the intrapulmonary instillation of preformed chemotactic mediators (C5fr and F-Met-Leu-Phe). By the use of 111Indium and 125Iodine labeling of homologous neutrophils (PMNs) and homologous albumin, respectively, it has been possible to obtain highly reproducible and quantitative parameters of the acute inflammatory response. The lung responds in a dose-dependent and time-dependent manner to the instillation of preformed chemotactic mediators. The quantitative parameters indicative of PMN influx were more prominent than changes in vascular permeability. The data obtained by the use of radiolabeled PMNs were confirmed by observation with light microscopy. Nonchemotactic substances such as human IgG, serum albumin, and C4 failed to induce inflammatory responses in lung. Interestingly, intact C5 instilled into lung was extremely phlogistic, apparently due to hydrolysis once within the lung. These studies provide an approach to reliable quantitative parameters of inflammatory reactions in the lung and emphasize the in vivo biologic effects of chemotactic mediators."}
{"id": "PMID:464028", "title": "Ultrastructural radioautography and cytochemistry of lead absorption.", "content": "Lead is a universal environmental contaminant absorbed largely through the gastrointestinal tract by unknown mechanisms. Because lead absorption is influenced by iron content in the body and diet, we used ultrastructural radioautography and cytochemistry to study absorption of physiologic lead doses in the rat duodenal epithelial cell and compared these findings to those previously reported for iron absorption. Rat duodenal loops exposed in vivo to 210Pb for 1 minute demonstrated the majority of labels on the microvilli, terminal web, and apical cytoplasm. Specimens exposed to radiolead for 10 minutes demonstrated more abundant labeling with a relative increase in labeling of epithelial cell mitochondria, nuclei and basal cytoplasm, as well as phagocytic cells, endothelial cells, and circulating erythrocytes of the lamina propria. Timm's sulfide-silver method localized trace metals in epithelial cells. After administration of lead, a significant increase in staining was observed in microvilli, mitochondria, non-membrane-bound cytoplasm, and nuclear chromatin. The rapid appearance of absorbed lead in epithelial cell mitochondria and nuclei, as well as phagocytic cells in the lamina propria, was distinctly different from that reported for absorbed iron and suggests different mechanisms for the subcellular transport of these cations. The combination of radioautography and Timm's sulfide-silver staining provides the specificity and resolution needed for ultrastructural evaluation of lead absorption and should be useful in further studies of lead metabolism.", "contents": "Ultrastructural radioautography and cytochemistry of lead absorption. Lead is a universal environmental contaminant absorbed largely through the gastrointestinal tract by unknown mechanisms. Because lead absorption is influenced by iron content in the body and diet, we used ultrastructural radioautography and cytochemistry to study absorption of physiologic lead doses in the rat duodenal epithelial cell and compared these findings to those previously reported for iron absorption. Rat duodenal loops exposed in vivo to 210Pb for 1 minute demonstrated the majority of labels on the microvilli, terminal web, and apical cytoplasm. Specimens exposed to radiolead for 10 minutes demonstrated more abundant labeling with a relative increase in labeling of epithelial cell mitochondria, nuclei and basal cytoplasm, as well as phagocytic cells, endothelial cells, and circulating erythrocytes of the lamina propria. Timm's sulfide-silver method localized trace metals in epithelial cells. After administration of lead, a significant increase in staining was observed in microvilli, mitochondria, non-membrane-bound cytoplasm, and nuclear chromatin. The rapid appearance of absorbed lead in epithelial cell mitochondria and nuclei, as well as phagocytic cells in the lamina propria, was distinctly different from that reported for absorbed iron and suggests different mechanisms for the subcellular transport of these cations. The combination of radioautography and Timm's sulfide-silver staining provides the specificity and resolution needed for ultrastructural evaluation of lead absorption and should be useful in further studies of lead metabolism."}
{"id": "PMID:464029", "title": "Treatment with a position feedback-controlled head stabilizer.", "content": "A position feedback-controlled head stabilizer has been developed to provide cerebral palsied individuals with resistive exercise to strengthen the neck musculature. This apparatus detects \"involuntary\" head motion and stabilizes the head by applying opposing forces; it also can be used to facilitate muscular contraction by resisting the subject's voluntary movements. The purpose of the present study was to determine whether voluntary head control in cerebral palsied individuals can be improved through systematic exercise using the stabilizer to strengthen the muscles of the neck and improve their balance of action. The findings support the author's contention that this is possible. The apparatus consists of a helmet and shoulder pads, interconnected so that the head is supported in the helmet by a manipulator arm. At its lower end, the manipulator arm is attached to the shoulder pad mounting frame via a gimbal assembly which allows head movement in two planes of tilt (pitch, or forward-and back, and roll, or side-to-side). Feedback control circuitry is so arranged that any deviation of the head from the desired position leads to actuation of pneumatic cylinders, which apply torques to the manipulator gimbal axes so as to oppose or conteract the incipient head movement. It is particularly significant that none of these patients participating in these experiments were at all apprehensive about or resisted being placed in the apparatus. (Even the youngest subject to use the apparatus--five year old-- did not mind being restrained by the shoulder pads or having his head gripped by helment.) While JG utilized the safety release valve quite often during the first few head control training sessions, he soon became confident enough in the action of the stabilizer that he did not even bother to grip the handle of the release valve. While DA had the action of safety valve explained and demonstrated for her, she never bothered to use it even from the outset of her experience with the stabilizer. Thus, it seems that the football shoulder pads use to stabilize the shoulders and the hockey helmet used to grip and manipulate the head actually make the apparatus attractive to younger patients, while the padding thereby provided makes it comfortable enough to be tolerated well by older individuals. And, the subject's knowing that he has an override control by means of which he can assert command over the entire system appears to be of psychological benefit in establishing confidence in both the apparatus and the investigator.", "contents": "Treatment with a position feedback-controlled head stabilizer. A position feedback-controlled head stabilizer has been developed to provide cerebral palsied individuals with resistive exercise to strengthen the neck musculature. This apparatus detects \"involuntary\" head motion and stabilizes the head by applying opposing forces; it also can be used to facilitate muscular contraction by resisting the subject's voluntary movements. The purpose of the present study was to determine whether voluntary head control in cerebral palsied individuals can be improved through systematic exercise using the stabilizer to strengthen the muscles of the neck and improve their balance of action. The findings support the author's contention that this is possible. The apparatus consists of a helmet and shoulder pads, interconnected so that the head is supported in the helmet by a manipulator arm. At its lower end, the manipulator arm is attached to the shoulder pad mounting frame via a gimbal assembly which allows head movement in two planes of tilt (pitch, or forward-and back, and roll, or side-to-side). Feedback control circuitry is so arranged that any deviation of the head from the desired position leads to actuation of pneumatic cylinders, which apply torques to the manipulator gimbal axes so as to oppose or conteract the incipient head movement. It is particularly significant that none of these patients participating in these experiments were at all apprehensive about or resisted being placed in the apparatus. (Even the youngest subject to use the apparatus--five year old-- did not mind being restrained by the shoulder pads or having his head gripped by helment.) While JG utilized the safety release valve quite often during the first few head control training sessions, he soon became confident enough in the action of the stabilizer that he did not even bother to grip the handle of the release valve. While DA had the action of safety valve explained and demonstrated for her, she never bothered to use it even from the outset of her experience with the stabilizer. Thus, it seems that the football shoulder pads use to stabilize the shoulders and the hockey helmet used to grip and manipulate the head actually make the apparatus attractive to younger patients, while the padding thereby provided makes it comfortable enough to be tolerated well by older individuals. And, the subject's knowing that he has an override control by means of which he can assert command over the entire system appears to be of psychological benefit in establishing confidence in both the apparatus and the investigator."}
{"id": "PMID:464030", "title": "Efficacy of myofeedback therapy in regaining control of lower extremity musculature following stroke.", "content": "The short-term efficacy (three months or less) of electromyographic biofeedback on the control and strength of six lower extremity muscles in twenty-two hemiplegic patients was investigated. The analysis of the data shows that patients receiving myofeedback therapy achieve a much larger increase in the control of muscle activity whereas there is no significant difference in the increase in strength.", "contents": "Efficacy of myofeedback therapy in regaining control of lower extremity musculature following stroke. The short-term efficacy (three months or less) of electromyographic biofeedback on the control and strength of six lower extremity muscles in twenty-two hemiplegic patients was investigated. The analysis of the data shows that patients receiving myofeedback therapy achieve a much larger increase in the control of muscle activity whereas there is no significant difference in the increase in strength."}
{"id": "PMID:464031", "title": "The C3 component of complement; gene frequency variation in Iraq.", "content": "Heterogeneity of gene frequencies in the polymorphism of the third component of complement among local samples in Iraq is analysed. It appears to derive essentially from population and not from regional differences.", "contents": "The C3 component of complement; gene frequency variation in Iraq. Heterogeneity of gene frequencies in the polymorphism of the third component of complement among local samples in Iraq is analysed. It appears to derive essentially from population and not from regional differences."}
{"id": "PMID:464032", "title": "Telemetered electromyography of flexor digitorum profundus and flexor digitorum superficialis in Pan troglodytes and implications for interpretation of the O. H. 7 hand.", "content": "The importance of knuckle-walking in the locomotor repertoire of African apes raises the possibility that the long digital flexors may be specially adapted more to meet the demands of ground quadrupedalism than those of suspension. To investigate this possibiltiy, the activities of the flexor digitorum superficialis and flexor digitorum profundus were studied by means of telemetered electromyography in three chimpanzees. Results clearly indicate that the fasciculi of the muscles to digits bearing weight in knuckle-walking are not called upon to contract in quadrupedal postures or in slow and moderately fast quadrupedal locomotion except to help clear the fingers from the ground as the forelimb begins its recovery stroke. At the most rapid speeds, a slight to moderate level of activity sometimes occurs in the latter half of stance phase. The long digital flexors display maximum and sustained activity during suspension. It is concluded that any role for these muscles in maintenance of stability at the metacarpophalangeal joints during knuckle-walking must be predominantly passive. Prominent markings for insertions of these muscles in a fossil hand (such as O.H. 7) suggest use of the forelimb in suspensory climbing behaviors.", "contents": "Telemetered electromyography of flexor digitorum profundus and flexor digitorum superficialis in Pan troglodytes and implications for interpretation of the O. H. 7 hand. The importance of knuckle-walking in the locomotor repertoire of African apes raises the possibility that the long digital flexors may be specially adapted more to meet the demands of ground quadrupedalism than those of suspension. To investigate this possibiltiy, the activities of the flexor digitorum superficialis and flexor digitorum profundus were studied by means of telemetered electromyography in three chimpanzees. Results clearly indicate that the fasciculi of the muscles to digits bearing weight in knuckle-walking are not called upon to contract in quadrupedal postures or in slow and moderately fast quadrupedal locomotion except to help clear the fingers from the ground as the forelimb begins its recovery stroke. At the most rapid speeds, a slight to moderate level of activity sometimes occurs in the latter half of stance phase. The long digital flexors display maximum and sustained activity during suspension. It is concluded that any role for these muscles in maintenance of stability at the metacarpophalangeal joints during knuckle-walking must be predominantly passive. Prominent markings for insertions of these muscles in a fossil hand (such as O.H. 7) suggest use of the forelimb in suspensory climbing behaviors."}
{"id": "PMID:464033", "title": "Placental alkaline phosphatase: population studies in Sardinia and data on the anthropological value of this genetic marker.", "content": "The analysis of human placental alkaline phosphatase polymorphism in Sardinia has shown a further difference in the genetic structure of this population in comparison with the populations of Continental Italy and Western Europe. Ethnic and geographic variations in world distribution of placental alkaline phosphatase gene frequencies suggest the considerable anthropological value of this genetic marker.", "contents": "Placental alkaline phosphatase: population studies in Sardinia and data on the anthropological value of this genetic marker. The analysis of human placental alkaline phosphatase polymorphism in Sardinia has shown a further difference in the genetic structure of this population in comparison with the populations of Continental Italy and Western Europe. Ethnic and geographic variations in world distribution of placental alkaline phosphatase gene frequencies suggest the considerable anthropological value of this genetic marker."}
{"id": "PMID:464034", "title": "The statistical treatment of correlated bilateral traits in the analysis of cranial material.", "content": "At present there is no standardized method to derive frequencies from bilateral non-metric traits. In this paper the commonly used methods are evaluated in light of statistical considerations and degree of sample preservation. In particular, we explore the question of dependence between sides for the bilateral traits. Several workers have tested for bilateral trait correlation in incorrect ways, confusing tests for differences in side frequencies with tests for independence. An easy method to test for independence, using the chi-squared test, is recommended. This test is used on 16 bilateral traits in a large sample of prehistoric carnia from Central California and significant dependence is found for all 16 of these traits. We suggest the traditional method of deriving frequencies be used. Both sides of the cranium should be considered, dividing the number of times the trait occurs by the number of sides available for observation. This method can be used even in poorly preserved samples. Side to side correlation may be compensated for by modifying the constants subtracted in the mean measure of divergence and in the expression for the approximate variance of the mean measure of divergence when the samples are drawn from identical populations.", "contents": "The statistical treatment of correlated bilateral traits in the analysis of cranial material. At present there is no standardized method to derive frequencies from bilateral non-metric traits. In this paper the commonly used methods are evaluated in light of statistical considerations and degree of sample preservation. In particular, we explore the question of dependence between sides for the bilateral traits. Several workers have tested for bilateral trait correlation in incorrect ways, confusing tests for differences in side frequencies with tests for independence. An easy method to test for independence, using the chi-squared test, is recommended. This test is used on 16 bilateral traits in a large sample of prehistoric carnia from Central California and significant dependence is found for all 16 of these traits. We suggest the traditional method of deriving frequencies be used. Both sides of the cranium should be considered, dividing the number of times the trait occurs by the number of sides available for observation. This method can be used even in poorly preserved samples. Side to side correlation may be compensated for by modifying the constants subtracted in the mean measure of divergence and in the expression for the approximate variance of the mean measure of divergence when the samples are drawn from identical populations."}
{"id": "PMID:464035", "title": "Sex assessment of the femur: a test of a new method.", "content": "Simple and multiple discriminant functions using mid-shaft femoral circumference for the determination of sex were used to test a sexing method recently proposed by Black. The method was able to correctly assign sex for 82% of the sample, which consisted of 115 North American White femora of verified age and sex. Circumference proved as accurate as any other criteria that have been used in sexing the femur. This suggests a usefulness beyond the limited archaelogical scope originally proposed by Black.", "contents": "Sex assessment of the femur: a test of a new method. Simple and multiple discriminant functions using mid-shaft femoral circumference for the determination of sex were used to test a sexing method recently proposed by Black. The method was able to correctly assign sex for 82% of the sample, which consisted of 115 North American White femora of verified age and sex. Circumference proved as accurate as any other criteria that have been used in sexing the femur. This suggests a usefulness beyond the limited archaelogical scope originally proposed by Black."}
{"id": "PMID:464036", "title": "Active and passive Ca movements in dog red blood cells and resealed ghosts.", "content": "Dog red blood cells accumulate Ca rapidly when deprived of substrate or cooled to 5 degrees C. The latter effect is reversible as the cells are rewarmed to body temperature. Resealed ghosts extrude Ca, provided ATP is incorporated in them. Passive fluxes of Ca are stimulated by Na on the opposite side of the membrane, consistent with a model for Ca-Na countertransport. Quinidine, cell shrinkage, and low pH--all known to suppress net Ca influx--have no accelerating effect on Ca efflux, thus validating earlier conclusions about the variability of the coupling ratio for Ca-Na exchange. The significance of these findings for cell volume regulation is discussed.", "contents": "Active and passive Ca movements in dog red blood cells and resealed ghosts. Dog red blood cells accumulate Ca rapidly when deprived of substrate or cooled to 5 degrees C. The latter effect is reversible as the cells are rewarmed to body temperature. Resealed ghosts extrude Ca, provided ATP is incorporated in them. Passive fluxes of Ca are stimulated by Na on the opposite side of the membrane, consistent with a model for Ca-Na countertransport. Quinidine, cell shrinkage, and low pH--all known to suppress net Ca influx--have no accelerating effect on Ca efflux, thus validating earlier conclusions about the variability of the coupling ratio for Ca-Na exchange. The significance of these findings for cell volume regulation is discussed."}
{"id": "PMID:464037", "title": "Lithium fluxes in human erythrocytes.", "content": "The contribution of four transport pathways to Li+ influx and efflux in human erythrocytes was determined quantitatively, using Li+ concentrations comparable to those found in vivo when Li+ is used as treatment for manic-depressive illness. All pathways were measured simultaneously on each subject's blood sample to avoid possible temporal variations in transport parameters. We found that Li+ efflux is 75% via countertransport and 25% via a leak. The bicarbonate-sensitive pathway accounts for 30% of influx while the remaining 70% is via a leak. The Na+-K+ pump makes no significant contribution to Li+ influx or efflux under physiological conditions. Li+ efflux for a given [Li+]i is 3-5 times the Li+ influx for the same [Li+]o. However, due to interindividual variations in Na+-Li+ counter-transport, Li+ efflux but not influx varies considerably among individuals.", "contents": "Lithium fluxes in human erythrocytes. The contribution of four transport pathways to Li+ influx and efflux in human erythrocytes was determined quantitatively, using Li+ concentrations comparable to those found in vivo when Li+ is used as treatment for manic-depressive illness. All pathways were measured simultaneously on each subject's blood sample to avoid possible temporal variations in transport parameters. We found that Li+ efflux is 75% via countertransport and 25% via a leak. The bicarbonate-sensitive pathway accounts for 30% of influx while the remaining 70% is via a leak. The Na+-K+ pump makes no significant contribution to Li+ influx or efflux under physiological conditions. Li+ efflux for a given [Li+]i is 3-5 times the Li+ influx for the same [Li+]o. However, due to interindividual variations in Na+-Li+ counter-transport, Li+ efflux but not influx varies considerably among individuals."}
{"id": "PMID:464038", "title": "Maintenance of ion concentration gradients in the cold in aorta from rat and ground squirrel.", "content": "Cell water and ionic content were measured in aortic smooth muscle from rats and ground squirrels during 48 h of incubation in oxygenated Krebs solution held at low temperatures. Cells from the ground squirrel, a hibernator, maintained sodium and potassium contents near normal levels during incubation at 7 degrees C. In sharp contrast, cells from the rat lost potassium and gained sodium with half times of 14 and 11 h, respectively. The [K] in cell water for the rat decreased 138 mM while [Na] in cell water increased about 148 mM. Cells from the hibernator tended to lose 10 mM K and gain 13 mM Na. Vascular smooth muscle of both the rat and squirrel was able to maintain K content at 17 degrees C. Tissue content of the divalent cations Ca and Mg did not change in vessels from either animal. The present findings suggest that aortic smooth muscle cells from a hibernator have adapted in such a way as to maintain ionic gradients upon prolonged exposure to low temperatures.", "contents": "Maintenance of ion concentration gradients in the cold in aorta from rat and ground squirrel. Cell water and ionic content were measured in aortic smooth muscle from rats and ground squirrels during 48 h of incubation in oxygenated Krebs solution held at low temperatures. Cells from the ground squirrel, a hibernator, maintained sodium and potassium contents near normal levels during incubation at 7 degrees C. In sharp contrast, cells from the rat lost potassium and gained sodium with half times of 14 and 11 h, respectively. The [K] in cell water for the rat decreased 138 mM while [Na] in cell water increased about 148 mM. Cells from the hibernator tended to lose 10 mM K and gain 13 mM Na. Vascular smooth muscle of both the rat and squirrel was able to maintain K content at 17 degrees C. Tissue content of the divalent cations Ca and Mg did not change in vessels from either animal. The present findings suggest that aortic smooth muscle cells from a hibernator have adapted in such a way as to maintain ionic gradients upon prolonged exposure to low temperatures."}
{"id": "PMID:464040", "title": "Length-dependent activation of in situ canine skeletal muscle.", "content": "This study was designed to assess the contribution of length-dependent activation to the peak isometric twitch tension developed and the maximal rate of tension development (dP/dt) of in situ canine skeletal muscle. Length-developed tension and length-dP/dt relationships were generated for the dog gastrocnemius-plantaris muscle group at three different levels of inotropic state as determined by stimulation frequency. These relationships were then normalized with respect to maximal developed tension and maximal dP/dt and the normalized curves were superimposed for comparison. At progressively shorter muscle lengths the augmentation of tension production by a given increment in inotropic state was greater as measured by either developed tension or dP/dt. Thus, a given change in muscle length produced a greater change in performance in less potentiated muscles. These findings are similar to those from studies of isolated cardiac muscle and illustrate the lack of independence between activational state and muscle length for in situ skeletal muscle.", "contents": "Length-dependent activation of in situ canine skeletal muscle. This study was designed to assess the contribution of length-dependent activation to the peak isometric twitch tension developed and the maximal rate of tension development (dP/dt) of in situ canine skeletal muscle. Length-developed tension and length-dP/dt relationships were generated for the dog gastrocnemius-plantaris muscle group at three different levels of inotropic state as determined by stimulation frequency. These relationships were then normalized with respect to maximal developed tension and maximal dP/dt and the normalized curves were superimposed for comparison. At progressively shorter muscle lengths the augmentation of tension production by a given increment in inotropic state was greater as measured by either developed tension or dP/dt. Thus, a given change in muscle length produced a greater change in performance in less potentiated muscles. These findings are similar to those from studies of isolated cardiac muscle and illustrate the lack of independence between activational state and muscle length for in situ skeletal muscle."}
{"id": "PMID:464041", "title": "Effect of chemotactic factors on calcium levels of rabbit neutrophils.", "content": "Studies were undertaken to provide a systematic investigation of the effect of the chemotactic factor, formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe), on the levels of total and exchangeable calcium in rabbit neutrophils. The 45Ca2+ specific activity of the cells was thus determined before and after stimulation by chemotactic factors. Total cell calcium was found to be constant in neutrophils equilibrated with 50-1,000 microM extracellular calcium but decreased by 30% at an extracellular calcium concentration of 5 microM. F-Met-Leu-Phe had little, if any, statistically significant effect on the level of total cell calcium. In contrast, F-Met-Leu-Phe affects greatly the steady-state levels of radioactive calcium in the neutrophils. The primary effect is an initial loss followed by an increase of cell-associated radiolabeled calcium. The extent and even the direction of the effect depends on the level of extracellular calcium as well as the concentration of the chemotactic factor and the duration of its interaction with the cell. The results are discussed in terms of the hypothesis that the binding of the chemotactic factor to its receptor leads to, among other things, a graded displacement of previously bound Ca2+.", "contents": "Effect of chemotactic factors on calcium levels of rabbit neutrophils. Studies were undertaken to provide a systematic investigation of the effect of the chemotactic factor, formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe), on the levels of total and exchangeable calcium in rabbit neutrophils. The 45Ca2+ specific activity of the cells was thus determined before and after stimulation by chemotactic factors. Total cell calcium was found to be constant in neutrophils equilibrated with 50-1,000 microM extracellular calcium but decreased by 30% at an extracellular calcium concentration of 5 microM. F-Met-Leu-Phe had little, if any, statistically significant effect on the level of total cell calcium. In contrast, F-Met-Leu-Phe affects greatly the steady-state levels of radioactive calcium in the neutrophils. The primary effect is an initial loss followed by an increase of cell-associated radiolabeled calcium. The extent and even the direction of the effect depends on the level of extracellular calcium as well as the concentration of the chemotactic factor and the duration of its interaction with the cell. The results are discussed in terms of the hypothesis that the binding of the chemotactic factor to its receptor leads to, among other things, a graded displacement of previously bound Ca2+."}
{"id": "PMID:464042", "title": "Energetics of sugar transport by isolated intestinal epithelial cells: effects of cytochalasin B.", "content": "The capability of isolated intestinal epithelial cells to establish concentration gradients of 3-O-methylglucose (3-OMG) by a Na+-dependent transport system is limited by concomitant function of a Na+-independent, facilitated diffusion transport system. Monosaccharides accumulated by the active system are continuously lost via the passive system, which acts to lower steady-state sugar gradients maintained by the cell. Cytochalasin B is a potent inhibitor of the passive system and allows the cells to establish a sugar gradient that is much higher than normal. When extracellular [3-OMG] is 1 mM, cytochalasin induces sugar accumulation ratios of 30-fold (+/- phlorizin) in contrast to control ratios of approximately 10-fold. When [3-OMG] is 0.1 mM, cytochalasin (0.1 mM) induces 40-fold accumulation ratios. When changes in extracellular sugar concentration are considered, steady-state concentration gradients observed are 70-fold. For a Na:sugar coupling stoichiometry of 1:1, gradients of this magnitude represent the approximate theoretical maximum for a transport system driven exclusively by the transmembrane electrochemical potential for Na+.", "contents": "Energetics of sugar transport by isolated intestinal epithelial cells: effects of cytochalasin B. The capability of isolated intestinal epithelial cells to establish concentration gradients of 3-O-methylglucose (3-OMG) by a Na+-dependent transport system is limited by concomitant function of a Na+-independent, facilitated diffusion transport system. Monosaccharides accumulated by the active system are continuously lost via the passive system, which acts to lower steady-state sugar gradients maintained by the cell. Cytochalasin B is a potent inhibitor of the passive system and allows the cells to establish a sugar gradient that is much higher than normal. When extracellular [3-OMG] is 1 mM, cytochalasin induces sugar accumulation ratios of 30-fold (+/- phlorizin) in contrast to control ratios of approximately 10-fold. When [3-OMG] is 0.1 mM, cytochalasin (0.1 mM) induces 40-fold accumulation ratios. When changes in extracellular sugar concentration are considered, steady-state concentration gradients observed are 70-fold. For a Na:sugar coupling stoichiometry of 1:1, gradients of this magnitude represent the approximate theoretical maximum for a transport system driven exclusively by the transmembrane electrochemical potential for Na+."}
{"id": "PMID:464046", "title": "Electrical activity of rat myocardial cells in culture: La3+-induced alterations.", "content": "Intracellular analysis of neonatal rat (1-2 day old) ventricular cells in culture shows that contracting myocardial cells exhibit an array of different patterns of spontaneous electrical activity. Resting membrane potentials varied between -40 mV and -98 mV. Our results indicate that some cultured cells show resting membrane potentials, overshoot, and total spike amplitude values comparable to those normally found in neonatal and adult rat heart. A low ratio of pacemaker (40%) to nonpacemaker cells (60%) and low incidence of hyperpolarizing after-potentials (35%) were found. La3+ application (0.1-4.0 mM) induced progressive cell depolarization, concomitant diminution in discharge frequency, and marked alteration of action potential configuration. A parallel decline in frequency and strength of rhythmic contractions was observed. Abolition of contractility occurred only in association with depolarization and complete disappearance of action potentials. Recovery of electrical and contractile activity followed medium replacement. Our results indicate that La3+ does not act as a specific excitation-contraction (E-C) uncoupler in the cultured cells but has multiple effects upon their normal electrical characteristics.", "contents": "Electrical activity of rat myocardial cells in culture: La3+-induced alterations. Intracellular analysis of neonatal rat (1-2 day old) ventricular cells in culture shows that contracting myocardial cells exhibit an array of different patterns of spontaneous electrical activity. Resting membrane potentials varied between -40 mV and -98 mV. Our results indicate that some cultured cells show resting membrane potentials, overshoot, and total spike amplitude values comparable to those normally found in neonatal and adult rat heart. A low ratio of pacemaker (40%) to nonpacemaker cells (60%) and low incidence of hyperpolarizing after-potentials (35%) were found. La3+ application (0.1-4.0 mM) induced progressive cell depolarization, concomitant diminution in discharge frequency, and marked alteration of action potential configuration. A parallel decline in frequency and strength of rhythmic contractions was observed. Abolition of contractility occurred only in association with depolarization and complete disappearance of action potentials. Recovery of electrical and contractile activity followed medium replacement. Our results indicate that La3+ does not act as a specific excitation-contraction (E-C) uncoupler in the cultured cells but has multiple effects upon their normal electrical characteristics."}
{"id": "PMID:464047", "title": "Calcium transport in human red blood cells under hypertonic conditions.", "content": "Calcium accumulation in intact human erythrocytes is enhanced by incubation in hypertonic solutions. Hypertonicity produces an increased permeability of the membrane to calcium that is time-dependent and occurs in the presence or absence of calcium. When hypertonically treated cells are incubated for more than 30 min in 300 mosmol/kg solutions the permeability of the membrane to calcium returns to basal values. Oligomycin inhibits the effect of hypertonicity on calcium uptake. The inhibitory action of oligomycin diminishes as the external sodium increases and can only be observed when the external concentration of potassium is at or below 3 mM. Low intracellular sodium and high intracellular potassium concentrations increase the uptake of calcium. It is concluded for human erythrocytes that 1) the increased permeability of the membrane to calcium produced by hypertonicity is a time-dependent, reversible phenomenon and is independent of calcium, 2) the increase in intracellular potassium concentration associated with hypertonic exposure is an important factor contributing to this response, and 3) interactions between calcium and components of the sodium-potassium transport system may account for the enhanced uptake of calcium produced by hypertonicity.", "contents": "Calcium transport in human red blood cells under hypertonic conditions. Calcium accumulation in intact human erythrocytes is enhanced by incubation in hypertonic solutions. Hypertonicity produces an increased permeability of the membrane to calcium that is time-dependent and occurs in the presence or absence of calcium. When hypertonically treated cells are incubated for more than 30 min in 300 mosmol/kg solutions the permeability of the membrane to calcium returns to basal values. Oligomycin inhibits the effect of hypertonicity on calcium uptake. The inhibitory action of oligomycin diminishes as the external sodium increases and can only be observed when the external concentration of potassium is at or below 3 mM. Low intracellular sodium and high intracellular potassium concentrations increase the uptake of calcium. It is concluded for human erythrocytes that 1) the increased permeability of the membrane to calcium produced by hypertonicity is a time-dependent, reversible phenomenon and is independent of calcium, 2) the increase in intracellular potassium concentration associated with hypertonic exposure is an important factor contributing to this response, and 3) interactions between calcium and components of the sodium-potassium transport system may account for the enhanced uptake of calcium produced by hypertonicity."}
{"id": "PMID:464048", "title": "Evidence that dog kidney is an endogenous source of histidine.", "content": "Net production or utilization (Qmet) of histidine by the kidney was evaluation or utilization (Qmet) of histidine by the kidney was evaluated in 11 normal and 8 chronically uremic female dogs during intravenous infusion, first of half-normal saline and then an amino acid solution containing L-histidine. Also, Qmet for histidine, alanine, beta-alanine, and carnosine (beta-alanyl-L-histidine) was determined during infusion of half-normal saline and then carnosine into the renal artery in seven normal dogs. During infusion of half-normal saline, Qmet histidine, calculated from the plasma data, was significantly positive in normal but not uremic dogs. Qmet histidine correlated with creatinine clearance. In uremic dogs, plasma histidine was decreased possibly due to decreased renal production and increased urinary excretion. Qmet histidine, calculated from the whole blood data during infusion of half-normal saline, was positive but not significantly so. During carnosine infusion, there was significant utilization of carnosine, and histidine production increased. Thus during fasting, the dog kidney appears to produce histidine, and histidine production increases with carnosine infusion. Hydrolysis of carnosine, possibly by carnosinase, may be a source of histidine released by the dog kidney.", "contents": "Evidence that dog kidney is an endogenous source of histidine. Net production or utilization (Qmet) of histidine by the kidney was evaluation or utilization (Qmet) of histidine by the kidney was evaluated in 11 normal and 8 chronically uremic female dogs during intravenous infusion, first of half-normal saline and then an amino acid solution containing L-histidine. Also, Qmet for histidine, alanine, beta-alanine, and carnosine (beta-alanyl-L-histidine) was determined during infusion of half-normal saline and then carnosine into the renal artery in seven normal dogs. During infusion of half-normal saline, Qmet histidine, calculated from the plasma data, was significantly positive in normal but not uremic dogs. Qmet histidine correlated with creatinine clearance. In uremic dogs, plasma histidine was decreased possibly due to decreased renal production and increased urinary excretion. Qmet histidine, calculated from the whole blood data during infusion of half-normal saline, was positive but not significantly so. During carnosine infusion, there was significant utilization of carnosine, and histidine production increased. Thus during fasting, the dog kidney appears to produce histidine, and histidine production increases with carnosine infusion. Hydrolysis of carnosine, possibly by carnosinase, may be a source of histidine released by the dog kidney."}
{"id": "PMID:464051", "title": "Acrosin of mouse spermatozoa.", "content": "Mouse spermatozoa possess a neutral proteinase, acrosin, that is to a large extent (70-80%) present in the zymogen (proacrosin) form. Acid extraction yields higher amounts of acrosin than detergent extraction. Synthetic inhibitor studies indicate that mouse acrosin has a serine and histidine at its active site and hydrolyzes the peptide bonds of lysine and arginine but of not phenylalanine. An inhibitor of acrosin is associated with mouse spermatozoa, capable of preventing the activity of at least 60% of all available acrosin. Acrosin activity is essential for fertilization because natural and synthetic inhibitors of mouse acrosin prevent the union of the gametes. Also, the relative inhibitory activity of synthetic agents toward acrosin runs approximately parallel to their antifertility activity. The percent of acrosin in the proacrosin form does not change after capacitating mouse spermatozoa in vitro.", "contents": "Acrosin of mouse spermatozoa. Mouse spermatozoa possess a neutral proteinase, acrosin, that is to a large extent (70-80%) present in the zymogen (proacrosin) form. Acid extraction yields higher amounts of acrosin than detergent extraction. Synthetic inhibitor studies indicate that mouse acrosin has a serine and histidine at its active site and hydrolyzes the peptide bonds of lysine and arginine but of not phenylalanine. An inhibitor of acrosin is associated with mouse spermatozoa, capable of preventing the activity of at least 60% of all available acrosin. Acrosin activity is essential for fertilization because natural and synthetic inhibitors of mouse acrosin prevent the union of the gametes. Also, the relative inhibitory activity of synthetic agents toward acrosin runs approximately parallel to their antifertility activity. The percent of acrosin in the proacrosin form does not change after capacitating mouse spermatozoa in vitro."}
{"id": "PMID:464052", "title": "Glucose-induced insulin release during acute and chronic hypoxia.", "content": "Glucose-induced insulin release was studied in young dogs during acute and chronic hypoxia, alone and in combination. Six experimental animals were rendered chronically hypoxic (PaO2, 43.4 +/- 0.5 torr) by creation of a right-to-left shunt at age 6-8 wk. Six control animals underwent sham procedures (PaO2, 85 +/- 2.2 torr) at the same age. During air breathing, glucose-induced plasma insulin increases were similar in chronically hypoxic and control animals. When severe hypoxia was acutely produced by ventilation with low-oxygen mixtures in experimental (PaO2, 23.7 +/- 1.7 torr) and control animals (PaO2, 26.3 +/- 1.0 torr), plasma insulin responses were markedly inhibited in both. On the other hand, acutely lowering oxygen tensions of control animals (PaO2, 37.5 +/- 1.4 torr) to levels close to those of air-breathing chronically hypoxic animals did not affect the insulin responses. These observations suggest that glucose-induced insulin release is inhibited by acute severe hypoxia despite previous chronic oxygen deficiency. In contrast, moderate hypoxia, acute or chronic, does not appear to affect the insulin response to a glucose load.", "contents": "Glucose-induced insulin release during acute and chronic hypoxia. Glucose-induced insulin release was studied in young dogs during acute and chronic hypoxia, alone and in combination. Six experimental animals were rendered chronically hypoxic (PaO2, 43.4 +/- 0.5 torr) by creation of a right-to-left shunt at age 6-8 wk. Six control animals underwent sham procedures (PaO2, 85 +/- 2.2 torr) at the same age. During air breathing, glucose-induced plasma insulin increases were similar in chronically hypoxic and control animals. When severe hypoxia was acutely produced by ventilation with low-oxygen mixtures in experimental (PaO2, 23.7 +/- 1.7 torr) and control animals (PaO2, 26.3 +/- 1.0 torr), plasma insulin responses were markedly inhibited in both. On the other hand, acutely lowering oxygen tensions of control animals (PaO2, 37.5 +/- 1.4 torr) to levels close to those of air-breathing chronically hypoxic animals did not affect the insulin responses. These observations suggest that glucose-induced insulin release is inhibited by acute severe hypoxia despite previous chronic oxygen deficiency. In contrast, moderate hypoxia, acute or chronic, does not appear to affect the insulin response to a glucose load."}
{"id": "PMID:464053", "title": "Effect of angiotensin II on parotid saliva secretion in conscious sheep.", "content": "Intravenous infusion of angiotensin II over the dose range 3-20 microgram/h for 15 min caused a dose-dependent reduction in parotid saliva secretion and increase in arterial blood pressure in conscious sheep. The blood levels of angiotensin II contrived by these infusions were probably within the physiological range for sheep. Infusion of angiotensin II (3 microgram/h) into the carotid artery ipsilateral to the parotid gland under study caused greater reduction in saliva secretion rate than an equivalent infusion of angiotensin II into the contralateral carotid artery. This result suggests a direct effect of angiotensin II at the parotid, possibly by a constrictor action on its vasculature or by altering water and electrolyte transport by the gland. In sodium-deplete sheep, intravenous infusion of the angiotensin antagonist saralasin (1 mg/h for 1 h) caused transient increase of saliva flow for 20-30 min. It is suggested that angiotensin II may have a physiological role in regulating parotid saliva secretion during sodium depletion.", "contents": "Effect of angiotensin II on parotid saliva secretion in conscious sheep. Intravenous infusion of angiotensin II over the dose range 3-20 microgram/h for 15 min caused a dose-dependent reduction in parotid saliva secretion and increase in arterial blood pressure in conscious sheep. The blood levels of angiotensin II contrived by these infusions were probably within the physiological range for sheep. Infusion of angiotensin II (3 microgram/h) into the carotid artery ipsilateral to the parotid gland under study caused greater reduction in saliva secretion rate than an equivalent infusion of angiotensin II into the contralateral carotid artery. This result suggests a direct effect of angiotensin II at the parotid, possibly by a constrictor action on its vasculature or by altering water and electrolyte transport by the gland. In sodium-deplete sheep, intravenous infusion of the angiotensin antagonist saralasin (1 mg/h for 1 h) caused transient increase of saliva flow for 20-30 min. It is suggested that angiotensin II may have a physiological role in regulating parotid saliva secretion during sodium depletion."}
{"id": "PMID:464054", "title": "Perinatal development of intestinal myoelectrical activity in dogs and sheep.", "content": "Both slow waves and bursts of spike potentials were recorded prior to birth in dogs and sheep. At 0l7-0.8 of term the slow-wave frequency of the jejunum did not exceed 12/min. It increased in a similar way during the last stage of fetal life in sheep and after birth in dogs. Adult values were attained 10-15 days after birth in the lamb but only by 40 days of age in the puppy. In both species, three stages of development of the spiking activity were identified. Stage 1, termed unorganized spiking activity, was converted in the fetal lamb at 0.8 of term to a fetal pattern (stage 2) characterized by cyclic 3- to 4-min periods of regular spiking occurring at 10- to 20-min intervals and propagated along a short intestinal segment. Stage 3, which corresponds to myoelectric complexes, occurred during the last 10 days of fetal life. In dogs, the fetal pattern (stage 2) was recorded from 5 days before to 15 days after birth. Stage 3 was seen in the 15-day-old neonate. The results suggest that the patterns of electrical activity seen in the last third of fetal life can be related to their function of mixing and absorbing but not expelling intestinal contents. They indicate that the electrical phenomena of the gastrointestinal tract are in accord with the overall greater maturity and independence of the newborn sheep compared to the dog.", "contents": "Perinatal development of intestinal myoelectrical activity in dogs and sheep. Both slow waves and bursts of spike potentials were recorded prior to birth in dogs and sheep. At 0l7-0.8 of term the slow-wave frequency of the jejunum did not exceed 12/min. It increased in a similar way during the last stage of fetal life in sheep and after birth in dogs. Adult values were attained 10-15 days after birth in the lamb but only by 40 days of age in the puppy. In both species, three stages of development of the spiking activity were identified. Stage 1, termed unorganized spiking activity, was converted in the fetal lamb at 0.8 of term to a fetal pattern (stage 2) characterized by cyclic 3- to 4-min periods of regular spiking occurring at 10- to 20-min intervals and propagated along a short intestinal segment. Stage 3, which corresponds to myoelectric complexes, occurred during the last 10 days of fetal life. In dogs, the fetal pattern (stage 2) was recorded from 5 days before to 15 days after birth. Stage 3 was seen in the 15-day-old neonate. The results suggest that the patterns of electrical activity seen in the last third of fetal life can be related to their function of mixing and absorbing but not expelling intestinal contents. They indicate that the electrical phenomena of the gastrointestinal tract are in accord with the overall greater maturity and independence of the newborn sheep compared to the dog."}
{"id": "PMID:464055", "title": "Nerve pathways in celiac plexus of the guinea pig.", "content": "In vitro preparations consisted of the right and left celiac, superior mesenteric and inferior mesenteric ganglia with attached extrinsic nerves, vasculature, mesentery, and colon. There were no systematic differences in membrane electrical properties (recorded intracellularly) between neurons in the different ganglia. Stimulation of associated nerve trunks produced graded synaptic responses in plexus neurons. Presynaptic fibers were found in splanchnic and mesenteric nerves. Input from celiac nerves dominated in the celiac galglia; input from the intermesenteric fibers dominated in the superior mesenteric ganglion. When the ganglia were attached to the entire colon, 33% of the neurons in the celiac and 54% in the superior mesenteric ganglion received a continuous excitatory synaptic input that was increased by distending the colon. This input was interrupted irreversibly by transsection of the mesenteric nerves. These results show that both the afferent and efferent pathways of a peripheral reflex arc are located in the mesenteric nerves and may mediate visceral reflexes between mechanoreceptors and sympathetic neurons in the colon.", "contents": "Nerve pathways in celiac plexus of the guinea pig. In vitro preparations consisted of the right and left celiac, superior mesenteric and inferior mesenteric ganglia with attached extrinsic nerves, vasculature, mesentery, and colon. There were no systematic differences in membrane electrical properties (recorded intracellularly) between neurons in the different ganglia. Stimulation of associated nerve trunks produced graded synaptic responses in plexus neurons. Presynaptic fibers were found in splanchnic and mesenteric nerves. Input from celiac nerves dominated in the celiac galglia; input from the intermesenteric fibers dominated in the superior mesenteric ganglion. When the ganglia were attached to the entire colon, 33% of the neurons in the celiac and 54% in the superior mesenteric ganglion received a continuous excitatory synaptic input that was increased by distending the colon. This input was interrupted irreversibly by transsection of the mesenteric nerves. These results show that both the afferent and efferent pathways of a peripheral reflex arc are located in the mesenteric nerves and may mediate visceral reflexes between mechanoreceptors and sympathetic neurons in the colon."}
{"id": "PMID:464056", "title": "Changes in gastrin levels, food intake, and duodenal mucosal growth during lactation.", "content": "Gastrointestinal epithelial cell proliferation is stimulated during lactation in the rat. Serum gastrin levels and food intake are also increased during lactation. We investigated the role of gastrin and food intake as possible mediators of duodenal mucosal growth during the first 15 days of lactation. As the lactation period progressed, significant increases in the following crypt properties were noted: 1) crypt length; 2) cells/crypt; 3) labeling index; and 4) dimensions of the proliferative zone. Maternal serum gastrin levels rose abruptly by the first day of birth and remained elevated throughout lactation. The increases in crypt cell proliferation significantly correlated with food intake but not with serum gastrin levels during lactation. Mucosal mass and villus-crypt dimensions were also significantly increased above virgin levels in lactating antrectomized rats. These results suggest that the increase in duodenal growth during lactation most probably is not mediated by postpartum hypergastrinemia and that the increase in cell proliferation may be a direct response to an enhancement in food intake.", "contents": "Changes in gastrin levels, food intake, and duodenal mucosal growth during lactation. Gastrointestinal epithelial cell proliferation is stimulated during lactation in the rat. Serum gastrin levels and food intake are also increased during lactation. We investigated the role of gastrin and food intake as possible mediators of duodenal mucosal growth during the first 15 days of lactation. As the lactation period progressed, significant increases in the following crypt properties were noted: 1) crypt length; 2) cells/crypt; 3) labeling index; and 4) dimensions of the proliferative zone. Maternal serum gastrin levels rose abruptly by the first day of birth and remained elevated throughout lactation. The increases in crypt cell proliferation significantly correlated with food intake but not with serum gastrin levels during lactation. Mucosal mass and villus-crypt dimensions were also significantly increased above virgin levels in lactating antrectomized rats. These results suggest that the increase in duodenal growth during lactation most probably is not mediated by postpartum hypergastrinemia and that the increase in cell proliferation may be a direct response to an enhancement in food intake."}
{"id": "PMID:464059", "title": "Renal tubular reabsorption in spontaneously hypertensive rats.", "content": "We characterized renal tubular reabsorption before and during acute expansion in anesthetized 12-wk-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Although mean arterial pressure was higher in euvolemic, nondiuretic SHR than in WKY, 158 vs. 114 mmHg, kidney and nephron glomerular filtration rate (GFR) as well as fluid reabsorption by the proximal convoluted tubule, loop of Henle, and distal convoluted tubule-collecting duct were similar. In euvolemic SHR with aortic constriction (SHR-AC), an acute decrease in renal perfusion pressure to 114 mmHg reduced sodium and water excretion. Kidney and nephron GFR and fluid reabsorption by segments along the nephron resembled values for SHR and WKY. Infusion of isotonic saline (3 ml.100 g body wt-1.h-1) produced similar increases in fractional sodium and water excretion by SHR and WKY, whereas SHR-AC exhibited a blunted natriuresis and diuresis. During expansion, fluid reabsorption by the nephron segments did not differ appreciably among the three groups. The effect(s) of perfusion pressure on reabsorption by superficial nephrons may be covert and was not unmasked, or may be manifested preferentially by deeper nephrons. We conclude that kidneys of SHR require a higher arterial pressure than kidneys of WKY to excrete a given amount of salt and water.", "contents": "Renal tubular reabsorption in spontaneously hypertensive rats. We characterized renal tubular reabsorption before and during acute expansion in anesthetized 12-wk-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Although mean arterial pressure was higher in euvolemic, nondiuretic SHR than in WKY, 158 vs. 114 mmHg, kidney and nephron glomerular filtration rate (GFR) as well as fluid reabsorption by the proximal convoluted tubule, loop of Henle, and distal convoluted tubule-collecting duct were similar. In euvolemic SHR with aortic constriction (SHR-AC), an acute decrease in renal perfusion pressure to 114 mmHg reduced sodium and water excretion. Kidney and nephron GFR and fluid reabsorption by segments along the nephron resembled values for SHR and WKY. Infusion of isotonic saline (3 ml.100 g body wt-1.h-1) produced similar increases in fractional sodium and water excretion by SHR and WKY, whereas SHR-AC exhibited a blunted natriuresis and diuresis. During expansion, fluid reabsorption by the nephron segments did not differ appreciably among the three groups. The effect(s) of perfusion pressure on reabsorption by superficial nephrons may be covert and was not unmasked, or may be manifested preferentially by deeper nephrons. We conclude that kidneys of SHR require a higher arterial pressure than kidneys of WKY to excrete a given amount of salt and water."}
{"id": "PMID:464060", "title": "Phosphate transport along the inner medullary collecting duct of the rat.", "content": "The question of phosphate transport along the collecting duct remains controversial inasmuch as no data from direct in vivo evaluation of this nephron segment have been reported. We measured net phosphate transport along the inner medullary collecting duct (IMCD) using the collecting duct microcatheterization technique in five groups of rats. In control rats no net phosphate transport was found and 9.4% of the fraction of filtered phosphate (FFP) entered the IMCD and was excreted. After acute thyroparathyroidectomy (TPTX) there was a striking reduction in the FFP entering the IMCD, 1.8%, and significant reabsorption occurred, 0.5% being excreted. With acute TPTX and parathormone infusion, delivery increased to 33% without significant change along the IMCD. With acute TPTX and phosphate infusion, delivery was increased to control levels but no change was found in net phosphate transport. In rats studied 5-7 days after uninephrectomy alone, phosphate delivery was greater than in control, 25%, and no net phosphate transport was found. These studies demonstrate that phosphate absorption occurs along the IMCD in acutely TPTX rats when the delivery of phosphate to the IMCD is markedly reduced. The increase in phosphaturia which occurs after a reduction in renal mass cannot be accounted for by changes in net phosphate transport along the IMCD.", "contents": "Phosphate transport along the inner medullary collecting duct of the rat. The question of phosphate transport along the collecting duct remains controversial inasmuch as no data from direct in vivo evaluation of this nephron segment have been reported. We measured net phosphate transport along the inner medullary collecting duct (IMCD) using the collecting duct microcatheterization technique in five groups of rats. In control rats no net phosphate transport was found and 9.4% of the fraction of filtered phosphate (FFP) entered the IMCD and was excreted. After acute thyroparathyroidectomy (TPTX) there was a striking reduction in the FFP entering the IMCD, 1.8%, and significant reabsorption occurred, 0.5% being excreted. With acute TPTX and parathormone infusion, delivery increased to 33% without significant change along the IMCD. With acute TPTX and phosphate infusion, delivery was increased to control levels but no change was found in net phosphate transport. In rats studied 5-7 days after uninephrectomy alone, phosphate delivery was greater than in control, 25%, and no net phosphate transport was found. These studies demonstrate that phosphate absorption occurs along the IMCD in acutely TPTX rats when the delivery of phosphate to the IMCD is markedly reduced. The increase in phosphaturia which occurs after a reduction in renal mass cannot be accounted for by changes in net phosphate transport along the IMCD."}
{"id": "PMID:464061", "title": "Feedback mediation of SNGFR autoregulation in hydropenic and DOCA- and salt-loaded rats.", "content": "Tubuloglomerular feedback (TGF) mediation of autoregulation was investigated by measuring the response of single nephron glomerular filtration rate (SNGFR) to changes in arterial pressure (AP) following acute or chronic TGF inhibition. In hydropenic rats with intact TGF, distal SNGFR was 25.0 +/- 1.2 (SE) and 23.9 +/- 1.4 nl/min at AP of 111 and 135 mmHg, respectively. In the same 20 nephrons during proximal tubular microinfusion of furosemide, distal SNGFR was 23.6 +/- 1.4 (n = 16) and 29.7 +/- 1.4 nl/min (n = 20) (P less than 0.001, n = 16) at 112 and 133 mmHg. When determined proximally, SNGFR was 25.6 +/- 1.0 and 29.5 +/- 0.9 nl/min (P less than 0.001, n = 31) at 112 and 157 mmHg; kidney GFR increased similarly. These data and the predictions of a GFR model were then used to estimate autoregulatory efficiency. This analysis indicated that partial autoregulation occurred during TGF inhibition. Therefore, TGF is an essential, but probably not the only, mechanism mediating SNGFR autoregulation.", "contents": "Feedback mediation of SNGFR autoregulation in hydropenic and DOCA- and salt-loaded rats. Tubuloglomerular feedback (TGF) mediation of autoregulation was investigated by measuring the response of single nephron glomerular filtration rate (SNGFR) to changes in arterial pressure (AP) following acute or chronic TGF inhibition. In hydropenic rats with intact TGF, distal SNGFR was 25.0 +/- 1.2 (SE) and 23.9 +/- 1.4 nl/min at AP of 111 and 135 mmHg, respectively. In the same 20 nephrons during proximal tubular microinfusion of furosemide, distal SNGFR was 23.6 +/- 1.4 (n = 16) and 29.7 +/- 1.4 nl/min (n = 20) (P less than 0.001, n = 16) at 112 and 133 mmHg. When determined proximally, SNGFR was 25.6 +/- 1.0 and 29.5 +/- 0.9 nl/min (P less than 0.001, n = 31) at 112 and 157 mmHg; kidney GFR increased similarly. These data and the predictions of a GFR model were then used to estimate autoregulatory efficiency. This analysis indicated that partial autoregulation occurred during TGF inhibition. Therefore, TGF is an essential, but probably not the only, mechanism mediating SNGFR autoregulation."}
{"id": "PMID:464062", "title": "Effect of fluoroacetate on the inhibitory action of ketone bodies and fatty acids on renal ammoniagenesis.", "content": "Renal cortical slices from acidotic dogs (NH4CL) were incubated at pH 7.0 with L-glutamine (1 and 5 mM) or L-glutamate (5 mM) with or without DL-beta-hydroxyburyrate 1 mM, acetoacetate 0.5 mM, as well as oleate, palmitate, octanoate, and crotonate 0.5 mM and in the presence or absence of fluoroacetate 0.05 mM. Fluoroacetate alone increased glutamine or glutamate uptake and ammoniagenesis whereas gluconeogenesis rose only when glutamine 5 mM or glutamate were used. Alanine production also rose by 30% when glutamine but not glutamate was used as substrate in the presence of fluoroacetate. Efficient blocking of the Krebs cycle at the aconitase level by fluoroacetate was evidenced by the release of citrate in the incubation medium and by a slight but significant decrease in oxygen consumption (10-20%). The marked decrease in glutamine uptake, ammoniagenesis, and gluconeogenesis induced by ketone bodies and fatty acids was completely corrected by addition of fluoroacetate. The present studies demonstrate that ketone bodies and fatty acids inhibit renal ammoniagenesis and gluconeogenesis in vitro through their oxidation in the mitochondria. They also suggest that direct transamination of glutamine into alanine (glutaminase II pathway) may be significant when oxidation of pyruvate is inhibited by fluoroacetate.", "contents": "Effect of fluoroacetate on the inhibitory action of ketone bodies and fatty acids on renal ammoniagenesis. Renal cortical slices from acidotic dogs (NH4CL) were incubated at pH 7.0 with L-glutamine (1 and 5 mM) or L-glutamate (5 mM) with or without DL-beta-hydroxyburyrate 1 mM, acetoacetate 0.5 mM, as well as oleate, palmitate, octanoate, and crotonate 0.5 mM and in the presence or absence of fluoroacetate 0.05 mM. Fluoroacetate alone increased glutamine or glutamate uptake and ammoniagenesis whereas gluconeogenesis rose only when glutamine 5 mM or glutamate were used. Alanine production also rose by 30% when glutamine but not glutamate was used as substrate in the presence of fluoroacetate. Efficient blocking of the Krebs cycle at the aconitase level by fluoroacetate was evidenced by the release of citrate in the incubation medium and by a slight but significant decrease in oxygen consumption (10-20%). The marked decrease in glutamine uptake, ammoniagenesis, and gluconeogenesis induced by ketone bodies and fatty acids was completely corrected by addition of fluoroacetate. The present studies demonstrate that ketone bodies and fatty acids inhibit renal ammoniagenesis and gluconeogenesis in vitro through their oxidation in the mitochondria. They also suggest that direct transamination of glutamine into alanine (glutaminase II pathway) may be significant when oxidation of pyruvate is inhibited by fluoroacetate."}
{"id": "PMID:464063", "title": "Adenosine content of skeletal muscle during active hyperemia and ischemic contraction.", "content": "If newly formed adenosine is the mediator of active hyperemia in skeletal muscle, tissue adenosine must increase and remain elevated during sustained muscle contraction. We tested this prediction using isolated canine anterior calf muscles. Muscle samples were obtained before and during contraction by punch biopsy and adenosine was measured by spectrophotometric enzyme assay. Tissue adenosine content didnot increase significantly above the precontraction level during either the 2- or 6-Hz contraction. We estimated (by infusing adenosine) that the adenosine concentration required to cause a dilation equal to that observed during 6-Hz contractions was 3.7 X 10(-5) M. We would have detected an increase in tissue adenosine if this concentration were established in as little as 10% of the skeletal muscle interstitium during free flow. These results indicate that adenosine released into the whole interstitial space is not the cause of free-flow exercise hyperemia.", "contents": "Adenosine content of skeletal muscle during active hyperemia and ischemic contraction. If newly formed adenosine is the mediator of active hyperemia in skeletal muscle, tissue adenosine must increase and remain elevated during sustained muscle contraction. We tested this prediction using isolated canine anterior calf muscles. Muscle samples were obtained before and during contraction by punch biopsy and adenosine was measured by spectrophotometric enzyme assay. Tissue adenosine content didnot increase significantly above the precontraction level during either the 2- or 6-Hz contraction. We estimated (by infusing adenosine) that the adenosine concentration required to cause a dilation equal to that observed during 6-Hz contractions was 3.7 X 10(-5) M. We would have detected an increase in tissue adenosine if this concentration were established in as little as 10% of the skeletal muscle interstitium during free flow. These results indicate that adenosine released into the whole interstitial space is not the cause of free-flow exercise hyperemia."}
{"id": "PMID:464064", "title": "Catecholamines and cardiac rhythms in the unanesthetized dog with chronic AV block.", "content": "The effects of epinephrine, norepinephrine, and isoproterenol were studied in the nonanesthetized dog with chronic atrioventricular block before and after blockade of cholinergic receptors. Epinephrine and norepinephrine reduced the atrial rate in a reflex manner, whereas isoproterenol increased it. In contrast, all three substances increased idioventricular rate. After atropine, atrial and ventricular rate increases were observed after administration of the three catecholamines. Moreover, blockade of cholinergic receptors led to an increase in ventricular acceleration induced by epinephrine and norepinephrine, but did not significantly modify the acceleration induced by isoproterenol. These results appear to indicate the possibility of the involvement (intensified by a reflex mechanism) of ventricular cholinergic innervation.", "contents": "Catecholamines and cardiac rhythms in the unanesthetized dog with chronic AV block. The effects of epinephrine, norepinephrine, and isoproterenol were studied in the nonanesthetized dog with chronic atrioventricular block before and after blockade of cholinergic receptors. Epinephrine and norepinephrine reduced the atrial rate in a reflex manner, whereas isoproterenol increased it. In contrast, all three substances increased idioventricular rate. After atropine, atrial and ventricular rate increases were observed after administration of the three catecholamines. Moreover, blockade of cholinergic receptors led to an increase in ventricular acceleration induced by epinephrine and norepinephrine, but did not significantly modify the acceleration induced by isoproterenol. These results appear to indicate the possibility of the involvement (intensified by a reflex mechanism) of ventricular cholinergic innervation."}
{"id": "PMID:464066", "title": "Determinations of blood flow and shunting of 9- and 15-micrometer spheres in regional beds.", "content": "In 17 pentobarbitalized dogs, the shunting of 15-micrometer and 9-micrometer microspheres was studied in the brain, myocardium, kidney, intestine, and lung. The veins of these organs were catheterized for constant blood withdrawal for 2 min by direct venipuncture. The ratio of microsphere radioactivity in the venous blood to that in the arterial blood gave the shunting of microspheres by the venous sampling technique. The 15-micrometer microspheres showed 2% or less shunting for all organs studied, whereas the 9-micrometer microspheres had shunting ranging from 3% in the coronary sinus to 24% in the portal vein. The shunting of 9-micrometer microspheres was also calculated from direct tissue counting, where the 15-micrometer spheres were considered to be completely entrapped. The results of direct tissue counting indicate that the 2-min venous sampling underestimates microsphere shunting. CO2 administration increased significantly the shunting of 9-micrometer spheres, whereas the shunting of 15-micrometer spheres determined by venous sampling remained less than 2%. Consideration of shunting indicates that the 15-micrometer microspheres might be more appropriate for regional organ blood flow measurements, including the myocardium.", "contents": "Determinations of blood flow and shunting of 9- and 15-micrometer spheres in regional beds. In 17 pentobarbitalized dogs, the shunting of 15-micrometer and 9-micrometer microspheres was studied in the brain, myocardium, kidney, intestine, and lung. The veins of these organs were catheterized for constant blood withdrawal for 2 min by direct venipuncture. The ratio of microsphere radioactivity in the venous blood to that in the arterial blood gave the shunting of microspheres by the venous sampling technique. The 15-micrometer microspheres showed 2% or less shunting for all organs studied, whereas the 9-micrometer microspheres had shunting ranging from 3% in the coronary sinus to 24% in the portal vein. The shunting of 9-micrometer microspheres was also calculated from direct tissue counting, where the 15-micrometer spheres were considered to be completely entrapped. The results of direct tissue counting indicate that the 2-min venous sampling underestimates microsphere shunting. CO2 administration increased significantly the shunting of 9-micrometer spheres, whereas the shunting of 15-micrometer spheres determined by venous sampling remained less than 2%. Consideration of shunting indicates that the 15-micrometer microspheres might be more appropriate for regional organ blood flow measurements, including the myocardium."}
{"id": "PMID:464068", "title": "Depressed responsiveness of the carotid sinus reflex in conscious newborn animals.", "content": "The responsiveness of the carotid sinus reflex was evaluated by comparing the effects of bilateral carotid occlusion (BCO) in conscious adult dogs and puppies on measurements of arterial pressure, cardiac output, heart rate, and calculations of total peripheral resistance (TPR). In eight adult dogs, BCO increased mean arterial pressure by 57 +/- 6%, TPR by 48 +/- 5%, and heart rate by 45 +/- 15%. In puppies, BCO induced smaller increases (P less than 0.05) in mean arterial pressure (30 +/- 5%) and TPR (29 +/- 4%), while heart rate did not change. After elimination of opposing vagal and aortic baroreceptor reflexes, the differences in responses to BCO of mean arterial pressure and TPR between adults and newborns were even greater. Thus, the carotid baroreceptor reflex appears to be depressed in the newborn when compared with the fully developed reflex in the normal, conscious adult.", "contents": "Depressed responsiveness of the carotid sinus reflex in conscious newborn animals. The responsiveness of the carotid sinus reflex was evaluated by comparing the effects of bilateral carotid occlusion (BCO) in conscious adult dogs and puppies on measurements of arterial pressure, cardiac output, heart rate, and calculations of total peripheral resistance (TPR). In eight adult dogs, BCO increased mean arterial pressure by 57 +/- 6%, TPR by 48 +/- 5%, and heart rate by 45 +/- 15%. In puppies, BCO induced smaller increases (P less than 0.05) in mean arterial pressure (30 +/- 5%) and TPR (29 +/- 4%), while heart rate did not change. After elimination of opposing vagal and aortic baroreceptor reflexes, the differences in responses to BCO of mean arterial pressure and TPR between adults and newborns were even greater. Thus, the carotid baroreceptor reflex appears to be depressed in the newborn when compared with the fully developed reflex in the normal, conscious adult."}
{"id": "PMID:464070", "title": "External assessment of myocardial metabolism with [11C]palmitate in rabbit hearts.", "content": "To determine whether the rate of fatty acid utilization, reflecting myocardial oxygen consumption under steady-state conditions, can be assessed noninvasively, we assayed positron emission from [11C]palmitate (100 muCi) in isolated rabbit hearts and after intra-atrial injection in vivo. In 11 isolated hearts, clearance of 11C-radioactivity during a monoexponential portion of residue detection curves correlated with tension-time index (TTI)(r = 0.86) and peak dP/dt (r = 0.89). Among 9 hearts prelabeled with [14C]palmitate, 14CO2 production from neutral lipid stores correlated with TTI (r = 0.83). In vivo the decline of 11C-radioactivity was 1st order and constant when physiological conditions were maintained constant (slope of the semilog plot = 0.099 +/- 0.002 (SE) in counts/min) (n = 2 animals, 7 injections). Decline of activity changed predictably when oxygen requirements were increased by administration of methoxamine. Thus, residue detection of [11C]palmitate 1) permits external assessment of global myocardial metabolism in vitro, and 2) reflects effects of interventions that alter the rate of substrate utilization in vivo, and should, therefore, permit comparison of rates of regional myocardial metabolism in patients with detection of radioactivity by positron-emission transaxial tomography.", "contents": "External assessment of myocardial metabolism with [11C]palmitate in rabbit hearts. To determine whether the rate of fatty acid utilization, reflecting myocardial oxygen consumption under steady-state conditions, can be assessed noninvasively, we assayed positron emission from [11C]palmitate (100 muCi) in isolated rabbit hearts and after intra-atrial injection in vivo. In 11 isolated hearts, clearance of 11C-radioactivity during a monoexponential portion of residue detection curves correlated with tension-time index (TTI)(r = 0.86) and peak dP/dt (r = 0.89). Among 9 hearts prelabeled with [14C]palmitate, 14CO2 production from neutral lipid stores correlated with TTI (r = 0.83). In vivo the decline of 11C-radioactivity was 1st order and constant when physiological conditions were maintained constant (slope of the semilog plot = 0.099 +/- 0.002 (SE) in counts/min) (n = 2 animals, 7 injections). Decline of activity changed predictably when oxygen requirements were increased by administration of methoxamine. Thus, residue detection of [11C]palmitate 1) permits external assessment of global myocardial metabolism in vitro, and 2) reflects effects of interventions that alter the rate of substrate utilization in vivo, and should, therefore, permit comparison of rates of regional myocardial metabolism in patients with detection of radioactivity by positron-emission transaxial tomography."}
{"id": "PMID:464071", "title": "Microvascular myogenic reaction in the wing of the intact unanesthetized bat.", "content": "Microvascular dimension and flow responses to stepwise changes in arterial and venous pressures, ranging from zero to +100 mmHg and zero to -75 mmHg have been recorded. Observations were made in arterioles, terminal arterioles, and precapillary sphincters in the wing web of intact, unanesthetized bats. The results show for all categories of vessels that with reduced transmural pressures there is a progressive increase in mean diameter and a decrease in rhythmic vasomotion rate. Flow changes are variable. For elevated transmural pressures there is a vasoconstriction with drastic flow reduction that is inconsistent with metabolic control. However, after prolonged elevation of pressure there is a progressive increase in flow, suggesting a \"metabolic escape\". Computed wall tension remains reasonably constant for a wide range of transmural pressures, suggesting that wall tension may be the controlled variable. These findings support the hypothesis of a myogenic reaction as a mechanism for maintenance of basal vascular tone in the intact unanesthetized bat.", "contents": "Microvascular myogenic reaction in the wing of the intact unanesthetized bat. Microvascular dimension and flow responses to stepwise changes in arterial and venous pressures, ranging from zero to +100 mmHg and zero to -75 mmHg have been recorded. Observations were made in arterioles, terminal arterioles, and precapillary sphincters in the wing web of intact, unanesthetized bats. The results show for all categories of vessels that with reduced transmural pressures there is a progressive increase in mean diameter and a decrease in rhythmic vasomotion rate. Flow changes are variable. For elevated transmural pressures there is a vasoconstriction with drastic flow reduction that is inconsistent with metabolic control. However, after prolonged elevation of pressure there is a progressive increase in flow, suggesting a \"metabolic escape\". Computed wall tension remains reasonably constant for a wide range of transmural pressures, suggesting that wall tension may be the controlled variable. These findings support the hypothesis of a myogenic reaction as a mechanism for maintenance of basal vascular tone in the intact unanesthetized bat."}
{"id": "PMID:464072", "title": "Myocardial performance in the newborn lamb.", "content": "We compared myocardial performance in newborn lambs over the first 3 wk of life with that of ewes. Heart rate was higher in newborn lambs than in ewes. The ratio of left ventricular preejection period to left ventricular ejection time was similar for newborns (0.31 +/- 0.02) and ewes (0.31 +/- 0.01). Cardiac output in ml/min per kg body weight in the newborn was 397 +/- 55 at 1 wk, 314 +/- 30 at 2 wk, and 274 +/- 10 at 3 wk, compared to 125 +/- 8 in ewes. Stroke work was comparable in both age groups. Left ventricular dP/dtmax averaged 3,723 mmHg/s in lambs compared to a mean value of 2,257 in ewes. These studies establish that the myocardium of newborn sheep contracts vigorously in vivo and equals or exceeds the level of performance of the myocardium of adult sheep, as estimated by a variety of indices of myocardial function.", "contents": "Myocardial performance in the newborn lamb. We compared myocardial performance in newborn lambs over the first 3 wk of life with that of ewes. Heart rate was higher in newborn lambs than in ewes. The ratio of left ventricular preejection period to left ventricular ejection time was similar for newborns (0.31 +/- 0.02) and ewes (0.31 +/- 0.01). Cardiac output in ml/min per kg body weight in the newborn was 397 +/- 55 at 1 wk, 314 +/- 30 at 2 wk, and 274 +/- 10 at 3 wk, compared to 125 +/- 8 in ewes. Stroke work was comparable in both age groups. Left ventricular dP/dtmax averaged 3,723 mmHg/s in lambs compared to a mean value of 2,257 in ewes. These studies establish that the myocardium of newborn sheep contracts vigorously in vivo and equals or exceeds the level of performance of the myocardium of adult sheep, as estimated by a variety of indices of myocardial function."}
{"id": "PMID:464073", "title": "Postnatal regulation of oxygen delivery: hematologic parameters of postnatal dogs.", "content": "Hematologic parameters influencing tissue oxygen delivery in dogs during the first 4 mo of life have been investigated. The rapid growth and increase in body temperature during this period imply an increased metabolic rate and increased tissue oxygen demand. Hemoglobin concentration and hematocrit decrease during the 1st mo following birth. The total red cell mass does not decrease during this period. The observed hemodilution can be attributed to an increasing plasma volume in the growing animal. The blood oxygen affinity decreases during this same period, resulting in a more effective tissue oxygen delivery. Erythropoiesis, as estimated from the percent circulating reticulocytes, decreases following birth and does not increase until 1 mo of postnatal life. The increase of erythropoietic activity during the 2nd mo of postnatal life coincides with an increase in red cell mass, hematocrit, and hemoglobin concentration.", "contents": "Postnatal regulation of oxygen delivery: hematologic parameters of postnatal dogs. Hematologic parameters influencing tissue oxygen delivery in dogs during the first 4 mo of life have been investigated. The rapid growth and increase in body temperature during this period imply an increased metabolic rate and increased tissue oxygen demand. Hemoglobin concentration and hematocrit decrease during the 1st mo following birth. The total red cell mass does not decrease during this period. The observed hemodilution can be attributed to an increasing plasma volume in the growing animal. The blood oxygen affinity decreases during this same period, resulting in a more effective tissue oxygen delivery. Erythropoiesis, as estimated from the percent circulating reticulocytes, decreases following birth and does not increase until 1 mo of postnatal life. The increase of erythropoietic activity during the 2nd mo of postnatal life coincides with an increase in red cell mass, hematocrit, and hemoglobin concentration."}
{"id": "PMID:464074", "title": "Postnatal changes in response of canine neonatal pulmonary arteries to histamine.", "content": "Postnatal development of histamine receptors in the canine pulmonary circulation was examined utilizing histamine cumulative dose-response curves of pulmonary arteries isolated from neonatal and adult dogs. The maximal contractile response to histamine was relatively low at birth (avg 0.069 g) and increased with postnatal age, reaching a maximum in the adult (avg 1.10 g). H2-receptor blockade with metiamide increased the contractile response to histamine during the first 2 wk of life (avg 0.38 g), suggesting H2-receptor dominance over H1-receptors in the newborn. Maximal developed tension, in response to KCl, gradually increased with postnatal age, suggesting progressive maturation of the smooth muscle response. In contrast to pulmonary arteries, isolated tracheal segments from puppies aged 1-5 days demonstrated large contractile responses (avg 5 g). Histamine (in microgram/g of wet wt of lung tissue) was absent in the lungs from 3rd trimester fetal dogs and rapidly increased over the first 2 wk of life, reaching a maximum in the adult. It is concluded that 1) the response of pulmonary arteries to histamine changes during the first 2 wk of life; 2) this change may reflect a decrease in H2-receptors and an increase in H1-receptors; 3) the contractile ability of pulmonary vascular smooth muscle increases with postnatal age; and 4) histamine is unavailable for physiological responses in the fetal dog pulmonary circulation.", "contents": "Postnatal changes in response of canine neonatal pulmonary arteries to histamine. Postnatal development of histamine receptors in the canine pulmonary circulation was examined utilizing histamine cumulative dose-response curves of pulmonary arteries isolated from neonatal and adult dogs. The maximal contractile response to histamine was relatively low at birth (avg 0.069 g) and increased with postnatal age, reaching a maximum in the adult (avg 1.10 g). H2-receptor blockade with metiamide increased the contractile response to histamine during the first 2 wk of life (avg 0.38 g), suggesting H2-receptor dominance over H1-receptors in the newborn. Maximal developed tension, in response to KCl, gradually increased with postnatal age, suggesting progressive maturation of the smooth muscle response. In contrast to pulmonary arteries, isolated tracheal segments from puppies aged 1-5 days demonstrated large contractile responses (avg 5 g). Histamine (in microgram/g of wet wt of lung tissue) was absent in the lungs from 3rd trimester fetal dogs and rapidly increased over the first 2 wk of life, reaching a maximum in the adult. It is concluded that 1) the response of pulmonary arteries to histamine changes during the first 2 wk of life; 2) this change may reflect a decrease in H2-receptors and an increase in H1-receptors; 3) the contractile ability of pulmonary vascular smooth muscle increases with postnatal age; and 4) histamine is unavailable for physiological responses in the fetal dog pulmonary circulation."}
{"id": "PMID:464076", "title": "Species differences in cardiac energetics.", "content": "The energy flux of rat, guinea pig, and cat papillary muscles was measured myothermically under resting, isometric, and isotonic conditions at 27 degrees C. Resting heat rate was highest in the smallest species and declined with body size. The slope of the isometric heat-stress relationship was constant across species, whereas the stress-independent heat component was least for rat muscles. The shape of the load enthalpy relationship was similar across species. Maximum mechanical efficiency, work-enthalpy, occurred with lighter loads than for skeletal muscle (approximately 0.2 Po). Rat muscle had the smallest enthalpy per beat and the highest active mechanical efficiency, but this advantage was nullified by the higher basal heat rate. The myothermic data are compared with cardiac oxygen consumption values in the literature and it is concluded, contrary to the deductions of common dimensional arguments, that cardiac energy expenditure across species is not directly proportional to heart rate. Reasons for this discrepancy are considered together with the likely contribution of cardiac metabolism (EH) to total body metabolism (EB). It seems likely that smaller species have lower EH/EB.", "contents": "Species differences in cardiac energetics. The energy flux of rat, guinea pig, and cat papillary muscles was measured myothermically under resting, isometric, and isotonic conditions at 27 degrees C. Resting heat rate was highest in the smallest species and declined with body size. The slope of the isometric heat-stress relationship was constant across species, whereas the stress-independent heat component was least for rat muscles. The shape of the load enthalpy relationship was similar across species. Maximum mechanical efficiency, work-enthalpy, occurred with lighter loads than for skeletal muscle (approximately 0.2 Po). Rat muscle had the smallest enthalpy per beat and the highest active mechanical efficiency, but this advantage was nullified by the higher basal heat rate. The myothermic data are compared with cardiac oxygen consumption values in the literature and it is concluded, contrary to the deductions of common dimensional arguments, that cardiac energy expenditure across species is not directly proportional to heart rate. Reasons for this discrepancy are considered together with the likely contribution of cardiac metabolism (EH) to total body metabolism (EB). It seems likely that smaller species have lower EH/EB."}
{"id": "PMID:464077", "title": "Resistance and volume changes caused by nitroprusside in the dog.", "content": "Changes in vascular volume caused by a pharmacologic agent are frequently inferred rather than directly measured. We investigated the effects of nitroprusside in 8 dogs divided into 2 groups: control and splenectomized. We anesthetized the dogs using pentobarbital, and surgically prepared a veno-right atrial bypass preparation whose controlled cardiac output and external reservoir allowed measurement of both changes in vascular resistance and changes in vascular volume. In both groups, blood pressure (mean +/- SD) decreased at each successive level of nitroprusside: 114 +/- 24 mmHg (base line), 101 +/- 19 mmHg (45 microgram/min), 90 +/- 16 mmHg (90 microgram/min), 81 +/- 17 mmHg (180 microgram/min), 68 +/- 18 mmHg (360 microgram/min). Nitroprusside caused a large and similar decrease in vascular resistance in both groups. In the control group, vascular volume increased above base line 5.5 +/- 2.7, 8.3 +/- 3.2, 11.6 +/- 2.9, and 14.7 +/- 3.5 ml/kg at each successive level of nitroprusside infusion, whereas in the splenectomized group vascular volume increased above base line 0.9 +/- 0.3, 2.5 +/- 1.0, 3.3 +/- 1.1, and 4.0 +/- 1.3 ml/kg at each successive level of nitroprusside infusion, but increased significantly less than the control group. We concluded that nitroprusside decreases vascular resistance and increases vascular volume and that the spleen is the major site of changes in vascular volume caused by nitroprusside.", "contents": "Resistance and volume changes caused by nitroprusside in the dog. Changes in vascular volume caused by a pharmacologic agent are frequently inferred rather than directly measured. We investigated the effects of nitroprusside in 8 dogs divided into 2 groups: control and splenectomized. We anesthetized the dogs using pentobarbital, and surgically prepared a veno-right atrial bypass preparation whose controlled cardiac output and external reservoir allowed measurement of both changes in vascular resistance and changes in vascular volume. In both groups, blood pressure (mean +/- SD) decreased at each successive level of nitroprusside: 114 +/- 24 mmHg (base line), 101 +/- 19 mmHg (45 microgram/min), 90 +/- 16 mmHg (90 microgram/min), 81 +/- 17 mmHg (180 microgram/min), 68 +/- 18 mmHg (360 microgram/min). Nitroprusside caused a large and similar decrease in vascular resistance in both groups. In the control group, vascular volume increased above base line 5.5 +/- 2.7, 8.3 +/- 3.2, 11.6 +/- 2.9, and 14.7 +/- 3.5 ml/kg at each successive level of nitroprusside infusion, whereas in the splenectomized group vascular volume increased above base line 0.9 +/- 0.3, 2.5 +/- 1.0, 3.3 +/- 1.1, and 4.0 +/- 1.3 ml/kg at each successive level of nitroprusside infusion, but increased significantly less than the control group. We concluded that nitroprusside decreases vascular resistance and increases vascular volume and that the spleen is the major site of changes in vascular volume caused by nitroprusside."}
{"id": "PMID:464079", "title": "Laryngeal reflex apnea in the chemodenervated newborn piglet.", "content": "Effects of peripheral arterial chemodernervation on laryngeal reflex-induced apnea were studied in 18 piglets of either sex varying in age from 4 to 63 days. The distal trachea was cannulated with a cuffed endotracheal tube to secure a free airway and permit ventilatory measurements with a pneumotachograph. The proximal trachea was used to introduce fluids into the larynx. Water elicited apnea, bradycardia, and arterial hypertension, whereas saline caused only transient disturbances. Electrical stimulation of the superior laryngeal nerves reproduced, and conduction anesthesia ablated, the effects of water in the larynx. Carotid body contribution to respiratory drive was assessed by the ventilatory responses to increased (100%) and decreased (10%) anbient oxygen concentration. These indicated significant peripheral chemoreceptor ventilatory activity from birth with little further change in the neonatal period. Ventilatory responses to oxygen were ablated by carotid chemodenervation, but there was no change in the duration of laryngeal reflex apnea. We conclude that attenuation of laryngeal-induced apnea during postnatal development is independent of peripheral chemoreceptor activity. Our findings may have relevance to the clinical problem of sudden infant death syndrome, in which carotid body abnormalities have recently been described.", "contents": "Laryngeal reflex apnea in the chemodenervated newborn piglet. Effects of peripheral arterial chemodernervation on laryngeal reflex-induced apnea were studied in 18 piglets of either sex varying in age from 4 to 63 days. The distal trachea was cannulated with a cuffed endotracheal tube to secure a free airway and permit ventilatory measurements with a pneumotachograph. The proximal trachea was used to introduce fluids into the larynx. Water elicited apnea, bradycardia, and arterial hypertension, whereas saline caused only transient disturbances. Electrical stimulation of the superior laryngeal nerves reproduced, and conduction anesthesia ablated, the effects of water in the larynx. Carotid body contribution to respiratory drive was assessed by the ventilatory responses to increased (100%) and decreased (10%) anbient oxygen concentration. These indicated significant peripheral chemoreceptor ventilatory activity from birth with little further change in the neonatal period. Ventilatory responses to oxygen were ablated by carotid chemodenervation, but there was no change in the duration of laryngeal reflex apnea. We conclude that attenuation of laryngeal-induced apnea during postnatal development is independent of peripheral chemoreceptor activity. Our findings may have relevance to the clinical problem of sudden infant death syndrome, in which carotid body abnormalities have recently been described."}
{"id": "PMID:464080", "title": "Abolition of hypoxic vagal bradycardia by lateral mesencephalic lesions in spinal cats.", "content": "To examine central sites that integrate the vagal bradycardia induced by hypoxia, heart rates were recorded continuously in spinal, precollicular, decerebrated cats during transient hypoxia induced by ventilation with 100% nitrogen. Bilateral lesions in the lateral mesencephalic reticular formation either decreased the extent of bradycardia or caused a reversal to tachycardia; anesthesia induced the same change. In contrast, bilateral lesions in the medial mesencephalic reticular formation failed to alter the bradycardia. Hence, the lateral mesencephalic reticular formation is essential for the appearance of hypoxia-induced vagal bradycardia.", "contents": "Abolition of hypoxic vagal bradycardia by lateral mesencephalic lesions in spinal cats. To examine central sites that integrate the vagal bradycardia induced by hypoxia, heart rates were recorded continuously in spinal, precollicular, decerebrated cats during transient hypoxia induced by ventilation with 100% nitrogen. Bilateral lesions in the lateral mesencephalic reticular formation either decreased the extent of bradycardia or caused a reversal to tachycardia; anesthesia induced the same change. In contrast, bilateral lesions in the medial mesencephalic reticular formation failed to alter the bradycardia. Hence, the lateral mesencephalic reticular formation is essential for the appearance of hypoxia-induced vagal bradycardia."}
{"id": "PMID:464082", "title": "Aortic wavelength as a determinant of the relation between heart rate and body size in mammals.", "content": "Measurements of aortic input impedance in the rat, dog, and man suggest a new hypothesis to explain the inverse correlation between rate and body size: that pulse wavelength and arterial length are matched in a way that minimizes cardiac work. The optimal rate is fast enough to avoid the high impedances produced by reflections at relatively low frequencies, yet slow enough to permit orderly excitation and recovery of the myocardium.", "contents": "Aortic wavelength as a determinant of the relation between heart rate and body size in mammals. Measurements of aortic input impedance in the rat, dog, and man suggest a new hypothesis to explain the inverse correlation between rate and body size: that pulse wavelength and arterial length are matched in a way that minimizes cardiac work. The optimal rate is fast enough to avoid the high impedances produced by reflections at relatively low frequencies, yet slow enough to permit orderly excitation and recovery of the myocardium."}
{"id": "PMID:464083", "title": "Renin and aldosterone secretions during hypovolemia in rats: relation to NaCl intake.", "content": "Plasma renin activities (PRA) and aldosterone concentrations increased in parallel over a wide range of plasma volume deficits produced in unanesthetized rats by extravascular administration of polyethylene glycol (PEG) solution. When PEG-treated rats were given water to drink, their intakes were proportional to PRA; when given water and 0.5 M NaCl, PRA and the steroid concentrations diminished concurrently in association with sodium consumption. Aldosterone concentrations and NaCl intakes were markedly enhanced after PEG treatment in rats maintained on a sodium-deficient diet for 4 days. On the other hand, a clear relation between PRA and water intake, and between circulating aldosterone levels and sodium intake, was not suggested by other experiments in this series. For example, bilateral nephrectomy abolished the rise in PRA during hypovolemia yet rats drank water normally. Moreover, aldosterone concentrations were substantially elevated by PEG treatment in the nephrectomized rats yet sodium appetite was abolished. These and other findings suggest that neither angiotensin nor aldosterone plays a prominent role in stimulating water and saline intakes during hypovolemia.", "contents": "Renin and aldosterone secretions during hypovolemia in rats: relation to NaCl intake. Plasma renin activities (PRA) and aldosterone concentrations increased in parallel over a wide range of plasma volume deficits produced in unanesthetized rats by extravascular administration of polyethylene glycol (PEG) solution. When PEG-treated rats were given water to drink, their intakes were proportional to PRA; when given water and 0.5 M NaCl, PRA and the steroid concentrations diminished concurrently in association with sodium consumption. Aldosterone concentrations and NaCl intakes were markedly enhanced after PEG treatment in rats maintained on a sodium-deficient diet for 4 days. On the other hand, a clear relation between PRA and water intake, and between circulating aldosterone levels and sodium intake, was not suggested by other experiments in this series. For example, bilateral nephrectomy abolished the rise in PRA during hypovolemia yet rats drank water normally. Moreover, aldosterone concentrations were substantially elevated by PEG treatment in the nephrectomized rats yet sodium appetite was abolished. These and other findings suggest that neither angiotensin nor aldosterone plays a prominent role in stimulating water and saline intakes during hypovolemia."}
{"id": "PMID:464084", "title": "O2 delivery to the pregnant uterus: its relationship to O2 consumption.", "content": "Normal sheep were studied at intervals of 3-5 days during the last weeks of pregnancy in order to evaluate variability in rate of O2 consumption (QO2) of the uterus in relation to several variables involved in delivery of O2 to the organ. Among-animal differences of uterine QO2 were statistically significant and directly related to birthweight of the lamb. Among-animal differences of uterine blood flow (UBF) and uterine arteriovenous O2 content difference [C(a-v)O2] also were statistically significant, though neither was related to birthweight, presumably since they tended to vary inversely with one another. In a given ewe relative magnitude of UBF and of C(a-v)O2 was related to maternal arterial O2 content (CaO2), day of pregnancy, and whether the animal carried singlets or twins. Variability in QO2 was most closely related to UBF, although its relations to C(a-v)O2 and CaO2 were significant also. These data suggest there are systematic relationships among variables involved in the delivery of O2 to the uterus of pregnant sheep.", "contents": "O2 delivery to the pregnant uterus: its relationship to O2 consumption. Normal sheep were studied at intervals of 3-5 days during the last weeks of pregnancy in order to evaluate variability in rate of O2 consumption (QO2) of the uterus in relation to several variables involved in delivery of O2 to the organ. Among-animal differences of uterine QO2 were statistically significant and directly related to birthweight of the lamb. Among-animal differences of uterine blood flow (UBF) and uterine arteriovenous O2 content difference [C(a-v)O2] also were statistically significant, though neither was related to birthweight, presumably since they tended to vary inversely with one another. In a given ewe relative magnitude of UBF and of C(a-v)O2 was related to maternal arterial O2 content (CaO2), day of pregnancy, and whether the animal carried singlets or twins. Variability in QO2 was most closely related to UBF, although its relations to C(a-v)O2 and CaO2 were significant also. These data suggest there are systematic relationships among variables involved in the delivery of O2 to the uterus of pregnant sheep."}
{"id": "PMID:464085", "title": "Independence of brain and body temperatures in flying American kestrels, Falco sparverius.", "content": "Brain and body temperatures were measured via small thermocouples implanted in the anterior hypothalami and colons, respectively, of five adult American kestrels (F. sparverius, mean mass 119 g) during descending flights in a wind tunnel at angles of 4 and 6 degrees below horizontal, at 10 m.s-1 air speed, and at 23 degrees C air temperature. For comparison, temperatures were recorded from resting birds at 22.5-36.1 degrees C air temperatures. Colonic (Tc) and hypothalamic (Th) temperatures both increased after the onset of flight; steady-state levels were attained after 1 min in the hypothalamus and after 5 or more min in the colon. The steady-state difference (delta T = Tc - Th) averaged 1.2 degrees C, higher by 0.5 degrees C than delta T in resting kestrels. The establishment of delta T during flight may be correlated with increased respiratory and corneal evaporation. The response apparently confines most stored body heat to noncranial regions, thus protecting brain tissue from thermal extremes.", "contents": "Independence of brain and body temperatures in flying American kestrels, Falco sparverius. Brain and body temperatures were measured via small thermocouples implanted in the anterior hypothalami and colons, respectively, of five adult American kestrels (F. sparverius, mean mass 119 g) during descending flights in a wind tunnel at angles of 4 and 6 degrees below horizontal, at 10 m.s-1 air speed, and at 23 degrees C air temperature. For comparison, temperatures were recorded from resting birds at 22.5-36.1 degrees C air temperatures. Colonic (Tc) and hypothalamic (Th) temperatures both increased after the onset of flight; steady-state levels were attained after 1 min in the hypothalamus and after 5 or more min in the colon. The steady-state difference (delta T = Tc - Th) averaged 1.2 degrees C, higher by 0.5 degrees C than delta T in resting kestrels. The establishment of delta T during flight may be correlated with increased respiratory and corneal evaporation. The response apparently confines most stored body heat to noncranial regions, thus protecting brain tissue from thermal extremes."}
{"id": "PMID:464086", "title": "Effects of carotid occlusion and left atrial stretch on supraoptic neurosecretory cells.", "content": "Acute experiments were performed on pentobarbital-anesthetized cats to determine whether atrial and carotid baroreceptors affected the same neurosecretory neurons in the supraoptic nucleus (SON) of the hypothalamus. The osmosensitivity of these neurons was also determined. Eighty-two SON neurons were antidromically identified and 63 of these increased their firing rates during complete occlusion of the right carotid artery. Further testing of these 63 neurons demonstrated that 56 were inhibited by directly stretching the left atrium and 61 were excited by intracarotid injection of hypertonic saline. Left atrial stretch greatly reduced the neuronal response to carotid occlusion. These results show that the activity of the majority of the antidromically identified SON neurons is altered by left atrial and carotid artery receptors as well as changes in plasma osmolality. Results are consistent with the known influence of these receptors on plasma vasopressin.", "contents": "Effects of carotid occlusion and left atrial stretch on supraoptic neurosecretory cells. Acute experiments were performed on pentobarbital-anesthetized cats to determine whether atrial and carotid baroreceptors affected the same neurosecretory neurons in the supraoptic nucleus (SON) of the hypothalamus. The osmosensitivity of these neurons was also determined. Eighty-two SON neurons were antidromically identified and 63 of these increased their firing rates during complete occlusion of the right carotid artery. Further testing of these 63 neurons demonstrated that 56 were inhibited by directly stretching the left atrium and 61 were excited by intracarotid injection of hypertonic saline. Left atrial stretch greatly reduced the neuronal response to carotid occlusion. These results show that the activity of the majority of the antidromically identified SON neurons is altered by left atrial and carotid artery receptors as well as changes in plasma osmolality. Results are consistent with the known influence of these receptors on plasma vasopressin."}
{"id": "PMID:464087", "title": "Body weight and body composition of male rats following hypothalamic lesions.", "content": "Carcass analyses were performed on 160 male rats maintaining reduced, normal, or elevated levels of body weight following lateral hypothalamic (LH), sham (control), or ventromedial hypothalamic (VMH) lesions, respectively. Extracted body lipid (ranging from 26 to 738% of the control mean) correlated highly (r = +0.95) with the level of maintained body weight (which ranged from 67 to 191% of control). Neither the nonfat solids (which ranged from 60 to 123% of control) contributed significantly to the variance in body weight (r = +0.01 and +0.06, respectively). Fat thus accounted for approximately 90% of the overall variance in body weight among LH, control, and VMH animals. Consideration of only the LH data, however, revealed a breakdown of this close covariance of body fat and weight. Fat mass correlated significantly with body weight in LH rats maintaining weight 0-12% below normal; but, at maintained body weights below 88%, the correlation between weight and fat in LH rats was only +0.07. Variation in lean body mass then better accounted for differences in body weight. The implications of these observations for existing lipostatic theories of weight regulation are discussed.", "contents": "Body weight and body composition of male rats following hypothalamic lesions. Carcass analyses were performed on 160 male rats maintaining reduced, normal, or elevated levels of body weight following lateral hypothalamic (LH), sham (control), or ventromedial hypothalamic (VMH) lesions, respectively. Extracted body lipid (ranging from 26 to 738% of the control mean) correlated highly (r = +0.95) with the level of maintained body weight (which ranged from 67 to 191% of control). Neither the nonfat solids (which ranged from 60 to 123% of control) contributed significantly to the variance in body weight (r = +0.01 and +0.06, respectively). Fat thus accounted for approximately 90% of the overall variance in body weight among LH, control, and VMH animals. Consideration of only the LH data, however, revealed a breakdown of this close covariance of body fat and weight. Fat mass correlated significantly with body weight in LH rats maintaining weight 0-12% below normal; but, at maintained body weights below 88%, the correlation between weight and fat in LH rats was only +0.07. Variation in lean body mass then better accounted for differences in body weight. The implications of these observations for existing lipostatic theories of weight regulation are discussed."}
{"id": "PMID:464091", "title": "Influence of dietary magnesium in experimental phosphate depletion: bone and soft tissue mineral changes.", "content": "We studied weanling rats fed 0.06% (group 1) and 0.10% (group II) magnesium (Mg) during phosphate depletion (PD) in order to evaluate the role of Mg in the bone, soft tissue, and serum changes of PD. The following results were obtained: 1) serum Mg remained stable in the face of a negative Mg balance; 2) the hypercalcemic and hypercalciuric response to PD was the same in both groups; 3) bone Mg content was decreased with PD in both groups and was associated with a significant decrease in bone calcium and phosphorus. We conclude that: 1) the hypomagnesemia of PD is dependent mainly on the dietary intake of Mg; 2) the hypercalcemia and hypercalciuria of PD are not caused by primary changes in Mg homeostasis; 3) low-dietary Mg during PD may cause a defect in soft tissue utilization of P in the growing rat.", "contents": "Influence of dietary magnesium in experimental phosphate depletion: bone and soft tissue mineral changes. We studied weanling rats fed 0.06% (group 1) and 0.10% (group II) magnesium (Mg) during phosphate depletion (PD) in order to evaluate the role of Mg in the bone, soft tissue, and serum changes of PD. The following results were obtained: 1) serum Mg remained stable in the face of a negative Mg balance; 2) the hypercalcemic and hypercalciuric response to PD was the same in both groups; 3) bone Mg content was decreased with PD in both groups and was associated with a significant decrease in bone calcium and phosphorus. We conclude that: 1) the hypomagnesemia of PD is dependent mainly on the dietary intake of Mg; 2) the hypercalcemia and hypercalciuria of PD are not caused by primary changes in Mg homeostasis; 3) low-dietary Mg during PD may cause a defect in soft tissue utilization of P in the growing rat."}
{"id": "PMID:464092", "title": "Electrical responses in cat adrenal cortex: possible relation to aldosterone secretion.", "content": "The effects of secretagogues for aldosterone release were studied on the membrane potential of cells in the adrenal cortex of the cat. Adrenal glands were excised, sliced, and continuously superfused. Membrane potentials were recorded from both zona glomerulosa and zona fasciculata-reticularis. Secretagogues, angiotensin II (1 microgram/ml) and 20 mM KCl, were found to depolarize cells rapidly. Ouabain (10(-5) M) also depolarized the membrane potential although the response was sluggish. Samples of the superfusate were collected and analyzed by radioimmunoassay for their aldosterone and cortisol content. Depolarizing concentrations of angiotensin II, KCl, and ouabain seemed to increase aldosterone release. Cortisol output was more variable. Saralasin blocked the effects of angiotensin II on the membrane potential. These experiments suggest that membrane depolarization plays a role in the stimulus-secretion coupling of mineral corticoids.", "contents": "Electrical responses in cat adrenal cortex: possible relation to aldosterone secretion. The effects of secretagogues for aldosterone release were studied on the membrane potential of cells in the adrenal cortex of the cat. Adrenal glands were excised, sliced, and continuously superfused. Membrane potentials were recorded from both zona glomerulosa and zona fasciculata-reticularis. Secretagogues, angiotensin II (1 microgram/ml) and 20 mM KCl, were found to depolarize cells rapidly. Ouabain (10(-5) M) also depolarized the membrane potential although the response was sluggish. Samples of the superfusate were collected and analyzed by radioimmunoassay for their aldosterone and cortisol content. Depolarizing concentrations of angiotensin II, KCl, and ouabain seemed to increase aldosterone release. Cortisol output was more variable. Saralasin blocked the effects of angiotensin II on the membrane potential. These experiments suggest that membrane depolarization plays a role in the stimulus-secretion coupling of mineral corticoids."}
{"id": "PMID:464093", "title": "Role of Ca2+ in genesis of lower esophageal sphincter tone and other active contractions.", "content": "The effects of absent or low Ca2+ (0.5 mM), verapamil, nifedipine, Na nitroprusside, theophylline, La2+, and ethanol on basal active tension (tone), \"off\" contractions, and carbachol contractions were studied in opossum lower esophageal sphincter strips. Incubation in Ca2+-free Ringer (0.1 mM EGTA) abolished tone and contractions. Low Ca2+, verapamil, nifedipine, and theophylline depressed tone more rapidly than \"off\" contractions. Only verapamil and nifedipine depressed carbachol contractions. Na nitroprusside rapidly depressed tone but left contractions unchanged. La3+ at 1 X 10(-3) M behaved like Ca2+-free incubation but produced sustained contractions with muscle stimulation. Ethanol depressed \"off\" contractions more than tone and did not affect carbachol-induced contractions. These results suggest that tone probably results from inward leak of Ca2+, whereas \"off\" contractions depend on release of Ca2+ sequestered in the cell by a mechanism not immediately dependent on increased Ca2+ influx. Carbachol may increase Ca2+ influx as well as utilize sequestered Ca2+. Nifedipine and verapamil may act to block both resting and stimulated Ca2+ influx. Na nitroprusside may act by increasing Ca2+ efflux. Ethanol may act by decreasing the availability of sequestered Ca2+ or by inhibiting the function of a mediator responsible for \"off\" contractions.", "contents": "Role of Ca2+ in genesis of lower esophageal sphincter tone and other active contractions. The effects of absent or low Ca2+ (0.5 mM), verapamil, nifedipine, Na nitroprusside, theophylline, La2+, and ethanol on basal active tension (tone), \"off\" contractions, and carbachol contractions were studied in opossum lower esophageal sphincter strips. Incubation in Ca2+-free Ringer (0.1 mM EGTA) abolished tone and contractions. Low Ca2+, verapamil, nifedipine, and theophylline depressed tone more rapidly than \"off\" contractions. Only verapamil and nifedipine depressed carbachol contractions. Na nitroprusside rapidly depressed tone but left contractions unchanged. La3+ at 1 X 10(-3) M behaved like Ca2+-free incubation but produced sustained contractions with muscle stimulation. Ethanol depressed \"off\" contractions more than tone and did not affect carbachol-induced contractions. These results suggest that tone probably results from inward leak of Ca2+, whereas \"off\" contractions depend on release of Ca2+ sequestered in the cell by a mechanism not immediately dependent on increased Ca2+ influx. Carbachol may increase Ca2+ influx as well as utilize sequestered Ca2+. Nifedipine and verapamil may act to block both resting and stimulated Ca2+ influx. Na nitroprusside may act by increasing Ca2+ efflux. Ethanol may act by decreasing the availability of sequestered Ca2+ or by inhibiting the function of a mediator responsible for \"off\" contractions."}
{"id": "PMID:464095", "title": "Does 5-hydroxytryptamine influence \"purinergic\" inhibitory neurons in the intestine?", "content": "Intrinsic inhibitory neurons to guinea pig taenia coli and small bowel circular muscle were activated by transmural electrical stimulation, and the postinhibitory contractile response of the muscle was utilized to evaluate whether or not the neuronal action of 5-hydroxytryptamine (5HT) was associated with the inhibitory neurons. The postinhibitory contractile responses of the small intestinal circular muscle were unaffected by 5HT. The 5HT antagonist methysergide also did not affect the poststimulus contractile response of the circular muscle. The amplitude and area under the contractile curve of the poststimulus contractile response of the taenia coli were reduced and the amplitude of the relaxation response to electrical stimulation was increased in one-half of the preparations after application of 5HT. Methysergide did not alter the poststimulus contractile response of the taenia coli. 5HT is implicated as a neurotransmitter substance for slow synaptic excitation within the enteric nervous system of the guinea pig small intestine; however, the 5HT synapses do not appear to be present on the \"purinergic\" inhibitory neurons nor on neurons that synaptically influence the inhibitory neurons.", "contents": "Does 5-hydroxytryptamine influence \"purinergic\" inhibitory neurons in the intestine? Intrinsic inhibitory neurons to guinea pig taenia coli and small bowel circular muscle were activated by transmural electrical stimulation, and the postinhibitory contractile response of the muscle was utilized to evaluate whether or not the neuronal action of 5-hydroxytryptamine (5HT) was associated with the inhibitory neurons. The postinhibitory contractile responses of the small intestinal circular muscle were unaffected by 5HT. The 5HT antagonist methysergide also did not affect the poststimulus contractile response of the circular muscle. The amplitude and area under the contractile curve of the poststimulus contractile response of the taenia coli were reduced and the amplitude of the relaxation response to electrical stimulation was increased in one-half of the preparations after application of 5HT. Methysergide did not alter the poststimulus contractile response of the taenia coli. 5HT is implicated as a neurotransmitter substance for slow synaptic excitation within the enteric nervous system of the guinea pig small intestine; however, the 5HT synapses do not appear to be present on the \"purinergic\" inhibitory neurons nor on neurons that synaptically influence the inhibitory neurons."}
{"id": "PMID:464096", "title": "Urinary concentrating ability during dehydration in the absence of vasopressin.", "content": "Despite the apparent absence of vasopressin (ADH), Brattleboro homozygotes [diabetes insipidus (DI) rats] can concentrate their urine when deprived of drinking water. Since other investigators have shown that reducing glomerular filtration rate (GFR) improves the concentrating ability of water-loaded dogs, the present studies were undertaken to quantify the magnitude and time course of changes in GFR during dehydration. Clearance experiments were performed in 10 conscious DI rats before and following 3, 6, 9, 12, 15, and 24 h of dehydration. Urine osmolality increased from 155.0 +/- 12.6 (SE) to 696.7 +/- 8.4 mosmol/kg H2O after 24 h. GFR averaged 984.3 +/- 79.6 microliters . min-1 . 100 g body wt-1 in the control phase, fell to about 80% of this value over the first 12 h of dehydration, and then declined to 27% at 24 h. The rats lost 20% of their body weight over the 24 h. The osmolality of the papillary tip averaged 896 +/- 44 mosmol/kg H2O at 24 h compared to a control value of 493 +/- 28. The lack of osmotic equilibration between urine and papillary interstitium suggests that dehydration did not appreciably increase the water permeability of the distal nephron. These experiments clearly show a progressive decline in GFR as urine becomes concentrated during dehydration in the absence of ADH; these events may or may not be causally related.", "contents": "Urinary concentrating ability during dehydration in the absence of vasopressin. Despite the apparent absence of vasopressin (ADH), Brattleboro homozygotes [diabetes insipidus (DI) rats] can concentrate their urine when deprived of drinking water. Since other investigators have shown that reducing glomerular filtration rate (GFR) improves the concentrating ability of water-loaded dogs, the present studies were undertaken to quantify the magnitude and time course of changes in GFR during dehydration. Clearance experiments were performed in 10 conscious DI rats before and following 3, 6, 9, 12, 15, and 24 h of dehydration. Urine osmolality increased from 155.0 +/- 12.6 (SE) to 696.7 +/- 8.4 mosmol/kg H2O after 24 h. GFR averaged 984.3 +/- 79.6 microliters . min-1 . 100 g body wt-1 in the control phase, fell to about 80% of this value over the first 12 h of dehydration, and then declined to 27% at 24 h. The rats lost 20% of their body weight over the 24 h. The osmolality of the papillary tip averaged 896 +/- 44 mosmol/kg H2O at 24 h compared to a control value of 493 +/- 28. The lack of osmotic equilibration between urine and papillary interstitium suggests that dehydration did not appreciably increase the water permeability of the distal nephron. These experiments clearly show a progressive decline in GFR as urine becomes concentrated during dehydration in the absence of ADH; these events may or may not be causally related."}
{"id": "PMID:464097", "title": "Renal vascular resistance and reactivity in the spontaneously hypertensive rat.", "content": "Renal vascular resistance is elevated in spontaneously hypertensive rats (SHR) when compared to normotensive control Wistar-Kyoto rats (WKY). The present study examined possible determinants of this raised vascular resistance in in situ autoperfused kidneys of pentobarbital-anesthetized, 12- to 16-wk-old SHR and WKY. Over a wide range of arterial pressures (30--100 mmHg) renal blood flow was consistently higher in WKY than in SHR. This relative flow difference was unchanged by acute renal denervation, with renal vascular resistance decreasing approximately 20% in both strains. Changes in renal vascular resistance to renal nerve stimulation and the administration of intra-arterial vasoactive hormones also were assessed. Vascular responses to renal nerve stimulation, tyramine, angiotensin II, and acetylcholine were similar in kidneys of the two strains, but reactivity to norepinephrine was significantly less in kidneys of SHR. It was concluded that elevated renal vascular resistance in the SHR does not result from an excessive neurogenic influence on the renal vasculature or from vascular hyperreactivity to norepinephrine or angiotensin II.", "contents": "Renal vascular resistance and reactivity in the spontaneously hypertensive rat. Renal vascular resistance is elevated in spontaneously hypertensive rats (SHR) when compared to normotensive control Wistar-Kyoto rats (WKY). The present study examined possible determinants of this raised vascular resistance in in situ autoperfused kidneys of pentobarbital-anesthetized, 12- to 16-wk-old SHR and WKY. Over a wide range of arterial pressures (30--100 mmHg) renal blood flow was consistently higher in WKY than in SHR. This relative flow difference was unchanged by acute renal denervation, with renal vascular resistance decreasing approximately 20% in both strains. Changes in renal vascular resistance to renal nerve stimulation and the administration of intra-arterial vasoactive hormones also were assessed. Vascular responses to renal nerve stimulation, tyramine, angiotensin II, and acetylcholine were similar in kidneys of the two strains, but reactivity to norepinephrine was significantly less in kidneys of SHR. It was concluded that elevated renal vascular resistance in the SHR does not result from an excessive neurogenic influence on the renal vasculature or from vascular hyperreactivity to norepinephrine or angiotensin II."}
{"id": "PMID:464098", "title": "Corticoid effects on angiotensin- and norepinephrine-induced proteinuria in rats.", "content": "Adrenalectomy is known to prevent the proteinuria induced by renin or angiotensin, but it is not clear whether the loss of glucocorticoids or mineralocorticoids is responsible. The problem was reinvestigated using dexamethasone and aldosterone, essentially pure glucocorticoid and mineralocorticoid, respectively. Dexamethasone treatment for 2--5 days completely restored the protein-uric response to angiotensin II or norepinephrine, but aldosterone did not, even though the dose and treatment were sufficient to induce changes in electrolyte excretion. Fractional sodium excretion was also increased by angiotensin II and norepinephrine in the dexamethasone-treated rats, but not in the aldosterone-treated rats. Both dexamethasone and aldosterone treatments restored the increase in filtration fraction, but the increase was not associated with proteinuria in some groups, and it is concluded that there is no causal relationship between increased filtration fraction and proteinuria. Reasons for considering binding of norepinephrine and angiotensin to the glomerular basement membrane as causal for the proteinuria and the hormonal requirements for such binding are discussed.", "contents": "Corticoid effects on angiotensin- and norepinephrine-induced proteinuria in rats. Adrenalectomy is known to prevent the proteinuria induced by renin or angiotensin, but it is not clear whether the loss of glucocorticoids or mineralocorticoids is responsible. The problem was reinvestigated using dexamethasone and aldosterone, essentially pure glucocorticoid and mineralocorticoid, respectively. Dexamethasone treatment for 2--5 days completely restored the protein-uric response to angiotensin II or norepinephrine, but aldosterone did not, even though the dose and treatment were sufficient to induce changes in electrolyte excretion. Fractional sodium excretion was also increased by angiotensin II and norepinephrine in the dexamethasone-treated rats, but not in the aldosterone-treated rats. Both dexamethasone and aldosterone treatments restored the increase in filtration fraction, but the increase was not associated with proteinuria in some groups, and it is concluded that there is no causal relationship between increased filtration fraction and proteinuria. Reasons for considering binding of norepinephrine and angiotensin to the glomerular basement membrane as causal for the proteinuria and the hormonal requirements for such binding are discussed."}
{"id": "PMID:464099", "title": "Effect of arsenite on renal tissue slice metabolism in chronic metabolic acidosis and alkalosis.", "content": "Tissue slices prepared from renal cortex of littermate dogs with chronic metabolic acidosis or alkalosis were incubated in media with or without arsenite and containing 1 mM L-[14C]glutamine or [1,5-14C]citrate. The presence of arsenite increased the concentration of alpha-ketoglutarate in slices by 5--20 times the values found without this inhibitor. alpha-Ketoglutarate concentrations in acidotic slices were 40% or more greater than those in alkalotic ones when arsenited was present. 14C incorporation into alpha-ketoglutarate was also increased manyfold by arsenite with either labeled glutamine or citrate as substrate. 14CO2 production from labeled glutamine by over 90% and from labeled citrate by over 75%; the difference between 14CO2 production by acidotic and alkalotic slices was greatly reduced or eliminated by arsenite. These results suggest that in chronic metabolic acidosis metabolism of both glutamine and citrate is stimulated at a site or sites preceding formation of alpha-ketoglutarate.", "contents": "Effect of arsenite on renal tissue slice metabolism in chronic metabolic acidosis and alkalosis. Tissue slices prepared from renal cortex of littermate dogs with chronic metabolic acidosis or alkalosis were incubated in media with or without arsenite and containing 1 mM L-[14C]glutamine or [1,5-14C]citrate. The presence of arsenite increased the concentration of alpha-ketoglutarate in slices by 5--20 times the values found without this inhibitor. alpha-Ketoglutarate concentrations in acidotic slices were 40% or more greater than those in alkalotic ones when arsenited was present. 14C incorporation into alpha-ketoglutarate was also increased manyfold by arsenite with either labeled glutamine or citrate as substrate. 14CO2 production from labeled glutamine by over 90% and from labeled citrate by over 75%; the difference between 14CO2 production by acidotic and alkalotic slices was greatly reduced or eliminated by arsenite. These results suggest that in chronic metabolic acidosis metabolism of both glutamine and citrate is stimulated at a site or sites preceding formation of alpha-ketoglutarate."}
{"id": "PMID:464100", "title": "Renin response to hemorrhage and hypotension in the aglomerular toadfish Opsanus tau.", "content": "Renal renin and the juxtaglomerular cells evolved in primitive bony fishes, whereas the macula densa emerged later in vertebrate phylogeny. We attempted to determine whether a renal arteriolar baroreceptor exists in the toadfish Opsanus tau, which possess renin and granulated cells in the kidneys, but lack glomeruli and macula densa. Cumulative hemorrhage of 1.5, 3, 6, 12, and 18 ml/kg, or a single massive bleeding from unanesthetized toadfish, kept in 50% seawater, caused an immediate and significant decrease in mean aortic pressure and stepwise increases (5-20 times) of plasma renin activity (PRA). Papaverine (10 mg/kg) caused hypotension and increased PRA. Minoxidil (6-12 mg/kg) neither decreased blood pressure nor increased PRA. The results suggest that toadfish respond to hemorrhage and acute hypotension with renin release despite the absence of a macula densa. It remains to be determined whether decreased renal perfusion pressure due to decreased dorsal aortic pressure stimulated the receptor in the granulated cells or whether the renal nerves may be involved.", "contents": "Renin response to hemorrhage and hypotension in the aglomerular toadfish Opsanus tau. Renal renin and the juxtaglomerular cells evolved in primitive bony fishes, whereas the macula densa emerged later in vertebrate phylogeny. We attempted to determine whether a renal arteriolar baroreceptor exists in the toadfish Opsanus tau, which possess renin and granulated cells in the kidneys, but lack glomeruli and macula densa. Cumulative hemorrhage of 1.5, 3, 6, 12, and 18 ml/kg, or a single massive bleeding from unanesthetized toadfish, kept in 50% seawater, caused an immediate and significant decrease in mean aortic pressure and stepwise increases (5-20 times) of plasma renin activity (PRA). Papaverine (10 mg/kg) caused hypotension and increased PRA. Minoxidil (6-12 mg/kg) neither decreased blood pressure nor increased PRA. The results suggest that toadfish respond to hemorrhage and acute hypotension with renin release despite the absence of a macula densa. It remains to be determined whether decreased renal perfusion pressure due to decreased dorsal aortic pressure stimulated the receptor in the granulated cells or whether the renal nerves may be involved."}
{"id": "PMID:464101", "title": "Noncholinergic vasodilator innervation in the feet of ducks.", "content": "Vasomotor responses to nerve stimulation were studied in the feet of Pekin ducks (Anas platyrhynchos) and domestic chickens (Gallus domesticus). The birds were killed and the feet isolated and perfused with a Krebs solution. Electrical stimulation of pedal nerves gave vasoconstrictor responses that were abolished by injecting guanethidine and by treating birds with reserpine. After guanethidine or reserpine, nerve stimulation resulted only in vasodilatation, which was unaffected by hexamethonium. Vasodilator responses to nerve stimulation were not blocked by hyoscine or atropine, which blocked responses to acetylcholine, nor by metiamide, which blocked vasodilatation in response to histamine. Responses to nerve stimulation were blocked by tetrodotoxin. Isoprenaline produced vasoconstriction that was blocked by phentolamine and also weak vasodilator responses that were antagonized by propranolol. It is concluded that the vasoconstrictor innervation is adrenergic. The identy of the vasodilator neurotransmitter is unknown; it is apparently not acetylcholine, a catecholamine, or histamine.", "contents": "Noncholinergic vasodilator innervation in the feet of ducks. Vasomotor responses to nerve stimulation were studied in the feet of Pekin ducks (Anas platyrhynchos) and domestic chickens (Gallus domesticus). The birds were killed and the feet isolated and perfused with a Krebs solution. Electrical stimulation of pedal nerves gave vasoconstrictor responses that were abolished by injecting guanethidine and by treating birds with reserpine. After guanethidine or reserpine, nerve stimulation resulted only in vasodilatation, which was unaffected by hexamethonium. Vasodilator responses to nerve stimulation were not blocked by hyoscine or atropine, which blocked responses to acetylcholine, nor by metiamide, which blocked vasodilatation in response to histamine. Responses to nerve stimulation were blocked by tetrodotoxin. Isoprenaline produced vasoconstriction that was blocked by phentolamine and also weak vasodilator responses that were antagonized by propranolol. It is concluded that the vasoconstrictor innervation is adrenergic. The identy of the vasodilator neurotransmitter is unknown; it is apparently not acetylcholine, a catecholamine, or histamine."}
{"id": "PMID:464102", "title": "Coronary dynamics and myocardial metabolism in the diabetic newborn lamb.", "content": "Diabetes mellitus (DM) was produced in 17 neonatal lambs by a single intravenous injection of alloxan monohydrate (150 mg/kg). All developed hyperglycemia (greater than 300 mg/100 ml) and glycosuria after 24 h, but none exhibited ketonuria or acidosis. Plasma insulin levels were reduced to about 20% of those appropriate for the plasma glucose levels. Glucose tolerance tests (intravenous) were also consistent with reduced insulin reserve. Measurements of left ventricular performance were obtained under constant hemodynamic conditions in 16 diabetics and did not differ from those in 10 normal lambs. However, myocardial uptake of glucose was sharply reduced and fatty acid uptake was less. Myocardial O2 consumption (MVO2) was unchanged. Coronary flow (CF) was significantly lower in the diabetics (P less than 0.001). Pressure-flow studies were also done and demonstrated a consistent reduction in CF at a given aortic pressure in DM compared with controls. These findings indicate that coronary vascular resistance is elevated in the lamb with DM, and this is not explained by a change in MVO2. Oxygen requirements of the diabetic hearts were satisfied by increased extraction.", "contents": "Coronary dynamics and myocardial metabolism in the diabetic newborn lamb. Diabetes mellitus (DM) was produced in 17 neonatal lambs by a single intravenous injection of alloxan monohydrate (150 mg/kg). All developed hyperglycemia (greater than 300 mg/100 ml) and glycosuria after 24 h, but none exhibited ketonuria or acidosis. Plasma insulin levels were reduced to about 20% of those appropriate for the plasma glucose levels. Glucose tolerance tests (intravenous) were also consistent with reduced insulin reserve. Measurements of left ventricular performance were obtained under constant hemodynamic conditions in 16 diabetics and did not differ from those in 10 normal lambs. However, myocardial uptake of glucose was sharply reduced and fatty acid uptake was less. Myocardial O2 consumption (MVO2) was unchanged. Coronary flow (CF) was significantly lower in the diabetics (P less than 0.001). Pressure-flow studies were also done and demonstrated a consistent reduction in CF at a given aortic pressure in DM compared with controls. These findings indicate that coronary vascular resistance is elevated in the lamb with DM, and this is not explained by a change in MVO2. Oxygen requirements of the diabetic hearts were satisfied by increased extraction."}
{"id": "PMID:464103", "title": "Flow and power output of right ventricle facing load with variable input impedance.", "content": "The output from the right ventricle (RV) was studied at different load impedances. Isolated cat hearts were perfused with Tyrode solution with erythrocytes. Coronary perfusion pressure and RV end-diastolic pressure were kept constant. The RV pumped into an artificial hydraulic load with independently variable resistance (R) and compliance (C). Mean RV flow (RVO) decreased after R increase or C reduction. For each heart, RVO and mean RV pressure were linearly related. The slope of the regression line is interpreted as an \"apparent source resistance\" (Rs). Rs was on average 3.4 X 10(3) (dyn.s.cm-5). The static hydraulic power output was maximum at a certain load R (Rm). Rm was C dependent at an average high C of 5 X 10(-5) dyn-1.cm5, Rm was 9.4 X 10(3) dyn.s.cm-5 on average and shifted to 5.4 X 10(3) at low C (avg 0.8 X 10(-5). Theoretical considerations show that Rm/Rs will be equal to total heart period divided by ejection period in the extreme case C leads to infinity, and Rm/Rs leads to 1 when C leads to 0. Experimentally, Rm/Rs was 2.4 (avg) for high C, and approached 1 for low C, which fits the theoretical predictions. The results indicate that high C facilitates the matching between the right heart and the vascular resistance in the lung.", "contents": "Flow and power output of right ventricle facing load with variable input impedance. The output from the right ventricle (RV) was studied at different load impedances. Isolated cat hearts were perfused with Tyrode solution with erythrocytes. Coronary perfusion pressure and RV end-diastolic pressure were kept constant. The RV pumped into an artificial hydraulic load with independently variable resistance (R) and compliance (C). Mean RV flow (RVO) decreased after R increase or C reduction. For each heart, RVO and mean RV pressure were linearly related. The slope of the regression line is interpreted as an \"apparent source resistance\" (Rs). Rs was on average 3.4 X 10(3) (dyn.s.cm-5). The static hydraulic power output was maximum at a certain load R (Rm). Rm was C dependent at an average high C of 5 X 10(-5) dyn-1.cm5, Rm was 9.4 X 10(3) dyn.s.cm-5 on average and shifted to 5.4 X 10(3) at low C (avg 0.8 X 10(-5). Theoretical considerations show that Rm/Rs will be equal to total heart period divided by ejection period in the extreme case C leads to infinity, and Rm/Rs leads to 1 when C leads to 0. Experimentally, Rm/Rs was 2.4 (avg) for high C, and approached 1 for low C, which fits the theoretical predictions. The results indicate that high C facilitates the matching between the right heart and the vascular resistance in the lung."}
{"id": "PMID:464104", "title": "Hemodynamics of central infusion of angiotensin II in normal and sodium-depleted dogs.", "content": "The intraventricular (IVT) administration of angiotensin II (AII) (100 ng.kg-1.min-1) produced significant elevations of arterial blood pressure in pentobarbital-anesthetized dogs on either normal or sodium-deficient diets. In both groups of dogs the intraventricular administration of AII caused comparable and significant elevations in blood pressure averaging 15 +/- 2 and 17 +/- 4 mmHg, respectively, within 10 min after onset of the infusion. The rises in blood pressure were due to increased peripheral resistance (2.87 +/- 1.20 vs. 1.67 +/- 0.43 units). At the peak of the pressor response bradycardia was a constant feature in sodium-depleted animals, but was not present in the normal ones. In both groups of dogs regional cerebral blood flow (rCBF), determined by the xenon-133 washout method, remained unchanged during the development of the pressor response, and peripheral plasma renin activity failed to increase in response to the central infusion of AII. In conclusion, sodium deprivation appears not to influence the sensitivity of the central AII receptor because comparable pressor responses and hemodynamic changes were obtained following the intraventricular administration of AII in both normal and sodium-depleted dogs.", "contents": "Hemodynamics of central infusion of angiotensin II in normal and sodium-depleted dogs. The intraventricular (IVT) administration of angiotensin II (AII) (100 ng.kg-1.min-1) produced significant elevations of arterial blood pressure in pentobarbital-anesthetized dogs on either normal or sodium-deficient diets. In both groups of dogs the intraventricular administration of AII caused comparable and significant elevations in blood pressure averaging 15 +/- 2 and 17 +/- 4 mmHg, respectively, within 10 min after onset of the infusion. The rises in blood pressure were due to increased peripheral resistance (2.87 +/- 1.20 vs. 1.67 +/- 0.43 units). At the peak of the pressor response bradycardia was a constant feature in sodium-depleted animals, but was not present in the normal ones. In both groups of dogs regional cerebral blood flow (rCBF), determined by the xenon-133 washout method, remained unchanged during the development of the pressor response, and peripheral plasma renin activity failed to increase in response to the central infusion of AII. In conclusion, sodium deprivation appears not to influence the sensitivity of the central AII receptor because comparable pressor responses and hemodynamic changes were obtained following the intraventricular administration of AII in both normal and sodium-depleted dogs."}
{"id": "PMID:464105", "title": "Vagal and sinoaortic reflexes in postural control of circulation and renin release.", "content": "Anesthetized cats were subjected to two 10-min trials of head-up tilting spaced 60 min apart. Bilateral cervical vagotomy, performed after the first tilting episode, did not affect the hemodynamic response to the change in posture, but significantyly and markedly reduced the increase in renin release from the innervated kidney. After sinoaortic denervation a marked and sustained arterial hypotension occurred during tilting, and the postural increase in renin release, though still limited to the innervated kidney, was even greater. When pressure homeostasis to tilting was disturbed, the renin release response was seen to be independent of the vagi, as it occurred after combined vagotomy and sinoaortic denervation. Maintenance of arterial pressure during tilting is mainly due to sinoaortic reflexes, whereas vagal reflexes are mostly responsible for the postural increase in renin release. The vagally independent release of renin observed when the cardiovascular response to tilting is impaired, is probably due to a direct action of arterial hypotension on intrarenal receptors, an action occurring only in innervated kidneys.", "contents": "Vagal and sinoaortic reflexes in postural control of circulation and renin release. Anesthetized cats were subjected to two 10-min trials of head-up tilting spaced 60 min apart. Bilateral cervical vagotomy, performed after the first tilting episode, did not affect the hemodynamic response to the change in posture, but significantyly and markedly reduced the increase in renin release from the innervated kidney. After sinoaortic denervation a marked and sustained arterial hypotension occurred during tilting, and the postural increase in renin release, though still limited to the innervated kidney, was even greater. When pressure homeostasis to tilting was disturbed, the renin release response was seen to be independent of the vagi, as it occurred after combined vagotomy and sinoaortic denervation. Maintenance of arterial pressure during tilting is mainly due to sinoaortic reflexes, whereas vagal reflexes are mostly responsible for the postural increase in renin release. The vagally independent release of renin observed when the cardiovascular response to tilting is impaired, is probably due to a direct action of arterial hypotension on intrarenal receptors, an action occurring only in innervated kidneys."}
{"id": "PMID:464107", "title": "Comparison of arterial wall mechanics in normotensive and spontaneously hypertensive rats.", "content": "Segments of carotid artery from Wistar (NW), Kyoto Wistar (WKY), and spontaneously hypertensive rats (SHR) were used to compare mechanical properties and connective tissue composition. Pressure-diameter measurements were made under conditions of active (5 microgram/ml norepinephrine) and passive (0 mM Ca2+ and 2 mM EGTA) smooth muscle. Systolic blood pressures averaged NW, 121 +/- 3; WKY, 124 +/- 4; and SHR, 187 +/- 5 MMHg. Passive mechanics were stiffest in SHR and most compliant in NW arteries. No differences in collagen-elastin ratio were found but collagen + elastin was lowest in SHR and highest in NW carotids. These results are not consistent with current concepts of the contribution of connective tissue elements to passive mechanics. Maximum stress development was NW, 561 +/- 49; WKY, 735 +/- 50; and SHR, 944 +/- 79 X 10(3) dyn/cm2. Diameter reductions to NE at 100 mmHg were NW, 17.6 +/- 2.4%; WKY, 16.7 +/- 2.0%; and SHR, 24.8 +/- 2.4%. The former suggests different contractile protein contents or more efficient intercellular force coupling in SHR. The latter suggests a more effective contractile apparatus as a result of stiffer passive muscle elements and/or a relatively larger wall thickness.", "contents": "Comparison of arterial wall mechanics in normotensive and spontaneously hypertensive rats. Segments of carotid artery from Wistar (NW), Kyoto Wistar (WKY), and spontaneously hypertensive rats (SHR) were used to compare mechanical properties and connective tissue composition. Pressure-diameter measurements were made under conditions of active (5 microgram/ml norepinephrine) and passive (0 mM Ca2+ and 2 mM EGTA) smooth muscle. Systolic blood pressures averaged NW, 121 +/- 3; WKY, 124 +/- 4; and SHR, 187 +/- 5 MMHg. Passive mechanics were stiffest in SHR and most compliant in NW arteries. No differences in collagen-elastin ratio were found but collagen + elastin was lowest in SHR and highest in NW carotids. These results are not consistent with current concepts of the contribution of connective tissue elements to passive mechanics. Maximum stress development was NW, 561 +/- 49; WKY, 735 +/- 50; and SHR, 944 +/- 79 X 10(3) dyn/cm2. Diameter reductions to NE at 100 mmHg were NW, 17.6 +/- 2.4%; WKY, 16.7 +/- 2.0%; and SHR, 24.8 +/- 2.4%. The former suggests different contractile protein contents or more efficient intercellular force coupling in SHR. The latter suggests a more effective contractile apparatus as a result of stiffer passive muscle elements and/or a relatively larger wall thickness."}
{"id": "PMID:464109", "title": "Autoregulation of superior mesenteric flow in fasted and fed dogs.", "content": "Pressure-flow relations in the intestinal vasculature were obtained by producing stepwise reductions of intestinal perfusion pressure with an adjustable metal clamp placed upon the descending thoracic aorta. To eliminate intervention of the baroreceptor reflex, systemic arterial pressure was maintained constant by a pressure compensation system connected to the two carotid arteries. In the fed dogs, autoregulatory vasodilatory responses to reduced perfusion pressure maintained flow within 5% of control in the 85-125 mmHg range. In 30% of the fed dogs, superior mesenteric flow actually increased as perfusion pressure was reduced in the pressure range 85-125 mmHg, indicating superregulation of flow in the intestinal vasculature. The degree of flow autoregulation in the fasted dog was less than in the fed dog. Moreover, superregulation of intestinal flow was not observed in any of the fasted animals. These data suggest an intimate link between parenchymal activity and local flow control in the small intestine.", "contents": "Autoregulation of superior mesenteric flow in fasted and fed dogs. Pressure-flow relations in the intestinal vasculature were obtained by producing stepwise reductions of intestinal perfusion pressure with an adjustable metal clamp placed upon the descending thoracic aorta. To eliminate intervention of the baroreceptor reflex, systemic arterial pressure was maintained constant by a pressure compensation system connected to the two carotid arteries. In the fed dogs, autoregulatory vasodilatory responses to reduced perfusion pressure maintained flow within 5% of control in the 85-125 mmHg range. In 30% of the fed dogs, superior mesenteric flow actually increased as perfusion pressure was reduced in the pressure range 85-125 mmHg, indicating superregulation of flow in the intestinal vasculature. The degree of flow autoregulation in the fasted dog was less than in the fed dog. Moreover, superregulation of intestinal flow was not observed in any of the fasted animals. These data suggest an intimate link between parenchymal activity and local flow control in the small intestine."}
{"id": "PMID:464110", "title": "Effect of sympathetic nerves on cerebral vessels during seizures.", "content": "The purpose of this study was to determine the effect of activation of sympathetic pathways during seizures on cerebral blood flow and integrity of the blood-brain barrier. We measured cerebral blood flow with microspheres and disruption of the blood-brain barrier with labeled albumin in cats. One cerebral hemisphere was denervated by cutting the superior cervical sympathetic trunk on one side. During bicuculline-induced seizures, superior cervical sympathetic nerve activity increased about threefold. Blood flow to the innervated hemibrain was significantly lower than flow to denervated hemibrain. However, in relation to the total increase in flow, this effect of nerves was minor. Blood-brain barrier permeability increased about sixfold during seizures, but there was no difference between the innervated and denervated sides of the brain. We conclude that sympathetic nerves attenuate the increase in cerebral blood flow during seizures, despite the increase in metabolism, but this effect is small. Activation of sympathetic nerves does not reduce disruption of the blood-brain barrier during seizures.", "contents": "Effect of sympathetic nerves on cerebral vessels during seizures. The purpose of this study was to determine the effect of activation of sympathetic pathways during seizures on cerebral blood flow and integrity of the blood-brain barrier. We measured cerebral blood flow with microspheres and disruption of the blood-brain barrier with labeled albumin in cats. One cerebral hemisphere was denervated by cutting the superior cervical sympathetic trunk on one side. During bicuculline-induced seizures, superior cervical sympathetic nerve activity increased about threefold. Blood flow to the innervated hemibrain was significantly lower than flow to denervated hemibrain. However, in relation to the total increase in flow, this effect of nerves was minor. Blood-brain barrier permeability increased about sixfold during seizures, but there was no difference between the innervated and denervated sides of the brain. We conclude that sympathetic nerves attenuate the increase in cerebral blood flow during seizures, despite the increase in metabolism, but this effect is small. Activation of sympathetic nerves does not reduce disruption of the blood-brain barrier during seizures."}
{"id": "PMID:464111", "title": "Myocardial relaxation. I. Effect of nitroprusside on the tension prolongation phenomenon.", "content": "The effects of nitroprusside and cyanide on myocardial relaxation were studied during hypoxia and reoxygenation of isolated rat papillary muscle, and during segmental ischemia and reperfusion in the intact dog heart. Nitroprusside did not affect isolated muscle performance before or during hypoxia. During reoxygenation of hypoxic muscles, the tension prolongation phenomenon (which characterizes abnormal or prolonged relaxation) was only slightly attenuated by the addition of nitroprusside to the muscle bath; in contrast, cyanide (at concentrations that did not prevent the return of tension) abolished tension prolongation during reoxygenation. During reperfusion of ischemic segments in intact hearts, the prolongation of segment tension was not affected by systemic administration of nitroprusside, but was abolished by intracoronary cyanide. Attenuation of the tension prolongation phenomenon by nitroprusside in the isolated muscle may be due to the liberation of cyanide. Inasmuch as nitroprusside did not affect the tension prolongation phenomenon in the intact heart, it is unlikely that the influence of this drug on left ventricular diastolic compliance is mediated through an alteration in the tension prolongation phenomenon.", "contents": "Myocardial relaxation. I. Effect of nitroprusside on the tension prolongation phenomenon. The effects of nitroprusside and cyanide on myocardial relaxation were studied during hypoxia and reoxygenation of isolated rat papillary muscle, and during segmental ischemia and reperfusion in the intact dog heart. Nitroprusside did not affect isolated muscle performance before or during hypoxia. During reoxygenation of hypoxic muscles, the tension prolongation phenomenon (which characterizes abnormal or prolonged relaxation) was only slightly attenuated by the addition of nitroprusside to the muscle bath; in contrast, cyanide (at concentrations that did not prevent the return of tension) abolished tension prolongation during reoxygenation. During reperfusion of ischemic segments in intact hearts, the prolongation of segment tension was not affected by systemic administration of nitroprusside, but was abolished by intracoronary cyanide. Attenuation of the tension prolongation phenomenon by nitroprusside in the isolated muscle may be due to the liberation of cyanide. Inasmuch as nitroprusside did not affect the tension prolongation phenomenon in the intact heart, it is unlikely that the influence of this drug on left ventricular diastolic compliance is mediated through an alteration in the tension prolongation phenomenon."}
{"id": "PMID:464112", "title": "Quantitative study of intramyocardial compression in the fibrillating heart.", "content": "Extravascular compression inhibits coronary blood flow in fibrillating hearts. Pressure-flow curves from spontaneously fibrillating hearts whose coronary arteries were maximally dilated were examined to see whether this inhibition involves a vascular waterfall mechanism as has been found in the beating heart. Waterfall behavior is indicated when pressure-flow curves are linear and experience a zero-flow intercept at pressures greater than venous pressure. Regional pressure-flow curves revealed a zero flow intercept of 28.4 mmHg for the inner quarter of the left ventricle, indicating that compression is quite high in that region. A zero-pressure intercept of only 15.1 was found at the outer quarter, which was not significantly different from venous pressure. We conclude that the spontaneously fibrillating heart experiences a gradient of compression falling from 28 mmHg at the subendocardium to near zero at the subepicardium.", "contents": "Quantitative study of intramyocardial compression in the fibrillating heart. Extravascular compression inhibits coronary blood flow in fibrillating hearts. Pressure-flow curves from spontaneously fibrillating hearts whose coronary arteries were maximally dilated were examined to see whether this inhibition involves a vascular waterfall mechanism as has been found in the beating heart. Waterfall behavior is indicated when pressure-flow curves are linear and experience a zero-flow intercept at pressures greater than venous pressure. Regional pressure-flow curves revealed a zero flow intercept of 28.4 mmHg for the inner quarter of the left ventricle, indicating that compression is quite high in that region. A zero-pressure intercept of only 15.1 was found at the outer quarter, which was not significantly different from venous pressure. We conclude that the spontaneously fibrillating heart experiences a gradient of compression falling from 28 mmHg at the subendocardium to near zero at the subepicardium."}
{"id": "PMID:464113", "title": "In vitro effects of desoxycorticosterone on vascular smooth muscle.", "content": "We present evidence in accord with the observations of S. Kalsner (Br. J. Pharmacol. 36: 582-593, 1969) that in the rabbit aorta, desoxycorticosterone (DOC) potentiates the contractile response to certain catecholamines by inhibiting their degradation by catechol-O-methyltransferase. In contrast, DOC depresses the contractile responses in rat aorta and tail arteries. To elucidate the mechanism of this depression the effect of DOC was evaluated under various conditions. DOC depressed the contractile response to epinephrine, phenylephrine, KCl, and angiotensin II. The depression was unaltered by ouabain or by a potassium-free solution, indicating that DOC did not produce its depression by altering Na-K-ATPase activity. The depression is unaltered in a chloride-free solution, demonstrating that the DOC effect is not caused by a change in membrane permeability to chloride. Radioisotope studies demonstrate that DOC does not alter membrane permeability to potassium. Removal of extracellular calcium with EGTA (ethylene glycol-bis (beta-aminoethyl ether) N, N'-tetraacetic acid) significantly reduced the magnitude of the DOC depression. Indirect evidence is presented suggesting that DOC might increase calcium binding to the plasma membrane, resulting in its stabilization and hence in a depression of the contractile response.", "contents": "In vitro effects of desoxycorticosterone on vascular smooth muscle. We present evidence in accord with the observations of S. Kalsner (Br. J. Pharmacol. 36: 582-593, 1969) that in the rabbit aorta, desoxycorticosterone (DOC) potentiates the contractile response to certain catecholamines by inhibiting their degradation by catechol-O-methyltransferase. In contrast, DOC depresses the contractile responses in rat aorta and tail arteries. To elucidate the mechanism of this depression the effect of DOC was evaluated under various conditions. DOC depressed the contractile response to epinephrine, phenylephrine, KCl, and angiotensin II. The depression was unaltered by ouabain or by a potassium-free solution, indicating that DOC did not produce its depression by altering Na-K-ATPase activity. The depression is unaltered in a chloride-free solution, demonstrating that the DOC effect is not caused by a change in membrane permeability to chloride. Radioisotope studies demonstrate that DOC does not alter membrane permeability to potassium. Removal of extracellular calcium with EGTA (ethylene glycol-bis (beta-aminoethyl ether) N, N'-tetraacetic acid) significantly reduced the magnitude of the DOC depression. Indirect evidence is presented suggesting that DOC might increase calcium binding to the plasma membrane, resulting in its stabilization and hence in a depression of the contractile response."}
{"id": "PMID:464114", "title": "Participation of slow inward current in the Purkinje fiber action potential overshoot.", "content": "We used microelectrode techniques to study the relationship of canine Purkinje fiber membrane potential and the action potential (AP) overshoot. At the maximum diastolic potential, -93.0 +/- 0.5 (SE) mV, AP overshoot was +37.7 +/- 0.4 mV. There was a range of membrane potentials (MP) less negative than the maximum diastolic potential from which action potentials were elicited with an overshoot greater than the control. Starting at an MP of less than -78.7 +/- 0.4 mV, AP overshoot was less than control. A maximum overshoot of +40.2 +/- 0.4 mV occurred at an MP of -85.4 +/- 0.4 mV. The relationship of the maximum upstroke velocity (Vmax) of phase 0 depolarization to MP was sigmoidal. Peak Vmax, 497 +/- 13 V/s, occurred at MP greater than or equal to -89.3 +/- 0.5 mV. The increase in overshoot was enhanced as perfusate [Ca2+] increased and decreased as [Ca2+] decreased. Slow-channel blocking agents and tetrodotoxin (TTX) depressed the peak of the curve relating overshoot to MP. TTX also decreased Vmax. The effect of TTX on overshoot but not on Vmax was reversed with Ca2+, 8.1 mM. The increase in overshoot for action potentials initiated during the terminal part of phase 3 was due to a slow, delayed component of the upstroke and appears to result from the slow inward current.", "contents": "Participation of slow inward current in the Purkinje fiber action potential overshoot. We used microelectrode techniques to study the relationship of canine Purkinje fiber membrane potential and the action potential (AP) overshoot. At the maximum diastolic potential, -93.0 +/- 0.5 (SE) mV, AP overshoot was +37.7 +/- 0.4 mV. There was a range of membrane potentials (MP) less negative than the maximum diastolic potential from which action potentials were elicited with an overshoot greater than the control. Starting at an MP of less than -78.7 +/- 0.4 mV, AP overshoot was less than control. A maximum overshoot of +40.2 +/- 0.4 mV occurred at an MP of -85.4 +/- 0.4 mV. The relationship of the maximum upstroke velocity (Vmax) of phase 0 depolarization to MP was sigmoidal. Peak Vmax, 497 +/- 13 V/s, occurred at MP greater than or equal to -89.3 +/- 0.5 mV. The increase in overshoot was enhanced as perfusate [Ca2+] increased and decreased as [Ca2+] decreased. Slow-channel blocking agents and tetrodotoxin (TTX) depressed the peak of the curve relating overshoot to MP. TTX also decreased Vmax. The effect of TTX on overshoot but not on Vmax was reversed with Ca2+, 8.1 mM. The increase in overshoot for action potentials initiated during the terminal part of phase 3 was due to a slow, delayed component of the upstroke and appears to result from the slow inward current."}
{"id": "PMID:464115", "title": "Cardiovascular changes during spontaneous micturition in conscious cats.", "content": "The hemodynamic changes occurring during spontaneous micturition were recorded in conscious cats. Arterial blood pressure was continuously measured by chronically implanted arterial catheter, heart rate (HR) by a cardiotachometer, and cardiac output (CO), superior mesenteric (MF), renal (RF), and external iliac blood flows (IF) by chronically implanted electromagnetic flow probes. Spontaneous micturition was accompanied by little change in mean arterial pressure (-9.7 +/- 0.7%), but by a marked decrease in HR (-49.0 +/- 1.2%) and CO (-28.6 +/- 2.5%), and therefore by a marked decrease in total peripheral conductance (-21.0 +/- 3.5%). Visceral and hindlimb blood flows were markedly reduced during micturition (MF, -34.7 +/- 2.1%; RF, -22.6 +/- 1.5%; and IF, -48.7 +/- 1.5%, respectively) due to a marked reduction in regional conductances in both these areas. The vasomotor changes in the regional circulations were prevented by local sympathectomy. Thus spontaneous micturition is associated with marked changes in cardiac function and systemic circulation. Cardiac output is decreased, but diffuse nervous systemic vasoconstriction compensates for this and provides maintenance of arterial blood pressure level.", "contents": "Cardiovascular changes during spontaneous micturition in conscious cats. The hemodynamic changes occurring during spontaneous micturition were recorded in conscious cats. Arterial blood pressure was continuously measured by chronically implanted arterial catheter, heart rate (HR) by a cardiotachometer, and cardiac output (CO), superior mesenteric (MF), renal (RF), and external iliac blood flows (IF) by chronically implanted electromagnetic flow probes. Spontaneous micturition was accompanied by little change in mean arterial pressure (-9.7 +/- 0.7%), but by a marked decrease in HR (-49.0 +/- 1.2%) and CO (-28.6 +/- 2.5%), and therefore by a marked decrease in total peripheral conductance (-21.0 +/- 3.5%). Visceral and hindlimb blood flows were markedly reduced during micturition (MF, -34.7 +/- 2.1%; RF, -22.6 +/- 1.5%; and IF, -48.7 +/- 1.5%, respectively) due to a marked reduction in regional conductances in both these areas. The vasomotor changes in the regional circulations were prevented by local sympathectomy. Thus spontaneous micturition is associated with marked changes in cardiac function and systemic circulation. Cardiac output is decreased, but diffuse nervous systemic vasoconstriction compensates for this and provides maintenance of arterial blood pressure level."}
{"id": "PMID:464116", "title": "Formation of angiotensin III from [des-Asp1]angiotensin I in the mesentric vasculature.", "content": "The effects of [des-Asp1]angiotensin I and angiotensin III on mesenteric blood flow were compared in 15 pentobarbital-anesthetized dogs. These agonists were administered as bolus injections directly into the vasculature supplied by the superior mesenteric artery. Both [des-Asp1]angiotensin I and angiotensin III produced dose-dependent decreases in mesenteric blood flow, with angiotensin III being more potent than [des-Asp1]angiotensin I at all doses tested. The constrictor responses to [des-Asp1]angiotensin I were markedly attenuated in the presence of an angiotensin-converting enzyme inhibitor (SQ20881); SQ20881 did not alter responses to angiotensin III or norepinephrine. The administration of [Ile7]angiotensin III (an angiotensin III antagonist) attenuated the responses to both [des-Asp1]angiotensin I and angiotensin III, without altering the responses to norepinephrine. These results suggest that the decrease in mesenteric blood flow produced by [des-Asp1]angiotensin I is largely caused by its local enzymatic conversion to angiotensin III. This conversion in one transit through the mesenteric vasculature is approximately 24%.", "contents": "Formation of angiotensin III from [des-Asp1]angiotensin I in the mesentric vasculature. The effects of [des-Asp1]angiotensin I and angiotensin III on mesenteric blood flow were compared in 15 pentobarbital-anesthetized dogs. These agonists were administered as bolus injections directly into the vasculature supplied by the superior mesenteric artery. Both [des-Asp1]angiotensin I and angiotensin III produced dose-dependent decreases in mesenteric blood flow, with angiotensin III being more potent than [des-Asp1]angiotensin I at all doses tested. The constrictor responses to [des-Asp1]angiotensin I were markedly attenuated in the presence of an angiotensin-converting enzyme inhibitor (SQ20881); SQ20881 did not alter responses to angiotensin III or norepinephrine. The administration of [Ile7]angiotensin III (an angiotensin III antagonist) attenuated the responses to both [des-Asp1]angiotensin I and angiotensin III, without altering the responses to norepinephrine. These results suggest that the decrease in mesenteric blood flow produced by [des-Asp1]angiotensin I is largely caused by its local enzymatic conversion to angiotensin III. This conversion in one transit through the mesenteric vasculature is approximately 24%."}
{"id": "PMID:464118", "title": "Myocardial adenosine salvage rates and restoration of ATP content following ischemia.", "content": "The isolated perfused rat heart was utilized to determine the maximum rate of adenosine incorporation into adenine nucleotides and the effect of ischemia on this rate. In aerobic hearts, the rates of [8-14C]adenosine incorporation into nucleotides in nanomoles/minute per gram dry tissue were ATP 34 +/- 2, ADP 6 +/- 0.4, AMP 3 +/- 0.3, and IMP, 1 +/- 0.2. Following ischemia these values were not significantly different except for the rate of incorporation into IMP, which doubled. The extent of adenosine deamination with one pass through the coronary vasculature was the same in aerobic and postischemic hearts: 2% and 7% of the perfusate adenosine was converted to hypoxanthine and inosine, respectively. These percentages were similar at 50, 100, and 200 micron adenosine. Perfusion of aerobic hearts for 5 h with adenosine did not change ATP concentrations. Therefore, [8-14C]adenosine incorporation into ATP in these hearts appeared to represent ATP turnover. In contrast, 5 h perfusion of postischemic hearts with adenosine restored ATP concentrations to control values. The synthesis rate calculated from the increase in ATP concentration was comparable to the synthesis rate calculated from [8-14C]adenosine incorporation. Thus, incorporation of [8-14C]adenosine into ATP in postischemic hearts represented net ATP synthesis.", "contents": "Myocardial adenosine salvage rates and restoration of ATP content following ischemia. The isolated perfused rat heart was utilized to determine the maximum rate of adenosine incorporation into adenine nucleotides and the effect of ischemia on this rate. In aerobic hearts, the rates of [8-14C]adenosine incorporation into nucleotides in nanomoles/minute per gram dry tissue were ATP 34 +/- 2, ADP 6 +/- 0.4, AMP 3 +/- 0.3, and IMP, 1 +/- 0.2. Following ischemia these values were not significantly different except for the rate of incorporation into IMP, which doubled. The extent of adenosine deamination with one pass through the coronary vasculature was the same in aerobic and postischemic hearts: 2% and 7% of the perfusate adenosine was converted to hypoxanthine and inosine, respectively. These percentages were similar at 50, 100, and 200 micron adenosine. Perfusion of aerobic hearts for 5 h with adenosine did not change ATP concentrations. Therefore, [8-14C]adenosine incorporation into ATP in these hearts appeared to represent ATP turnover. In contrast, 5 h perfusion of postischemic hearts with adenosine restored ATP concentrations to control values. The synthesis rate calculated from the increase in ATP concentration was comparable to the synthesis rate calculated from [8-14C]adenosine incorporation. Thus, incorporation of [8-14C]adenosine into ATP in postischemic hearts represented net ATP synthesis."}
{"id": "PMID:464119", "title": "Intramural PCO2: a reliable index of the severity of myocardial ischemic injury.", "content": "The present study was performed to evaluate the usefulness of changes in intramural oxygen (PmO2) and carbon dioxide (PmCO2) tensions shortly after coronary artery occlusion as indices of the severity of myocardial ischemic injury. In 14 open-chest, anesthetized dogs, a 60-min coronary artery occlusion was performed, during which PmO2 and PMCO2 were measured continuously with a mass spectrometer. Regional myocardial blood flow (RMBF) adjacent to the mass spectrometer probes was measured by the xenon-127 washout technique both before and 30 min after coronary artery occlusion. At the end of 60 min of occlusion, the dogs were killed, and biopsies for histological examination of 1-micron-thick sections were obtained from the tissue surrounding each mass spectometer probe. The decline in PmO2 during the 60-min occlusion bore no relationship either to the severity of ischemic injury as assessed by histological examination, or to the reduction of RMBF. In contrast, the magnitude of rise in PmCO2 during the 60 min of occlusion corresponded closely to both the severity of injury assessed histologically and the reduction of RMBF.", "contents": "Intramural PCO2: a reliable index of the severity of myocardial ischemic injury. The present study was performed to evaluate the usefulness of changes in intramural oxygen (PmO2) and carbon dioxide (PmCO2) tensions shortly after coronary artery occlusion as indices of the severity of myocardial ischemic injury. In 14 open-chest, anesthetized dogs, a 60-min coronary artery occlusion was performed, during which PmO2 and PMCO2 were measured continuously with a mass spectrometer. Regional myocardial blood flow (RMBF) adjacent to the mass spectrometer probes was measured by the xenon-127 washout technique both before and 30 min after coronary artery occlusion. At the end of 60 min of occlusion, the dogs were killed, and biopsies for histological examination of 1-micron-thick sections were obtained from the tissue surrounding each mass spectometer probe. The decline in PmO2 during the 60-min occlusion bore no relationship either to the severity of ischemic injury as assessed by histological examination, or to the reduction of RMBF. In contrast, the magnitude of rise in PmCO2 during the 60 min of occlusion corresponded closely to both the severity of injury assessed histologically and the reduction of RMBF."}
{"id": "PMID:464120", "title": "Laser scanner measurement of central segment performance in isolated cardiac muscle preparations.", "content": "A laser scanning system has been developed to facilitate the study of isolated cardiac muscle by excluding the possibly damaged tissue at the ends of the preparation. The laser scanner monitors the length of a central segment of the muscle that has been delineated by markers consisting of single loops of 8-0 silk suture. Focused laser light traverses the length of the muscle at 150 Hz. Scattered light is detected by a photodiode and the length of the central segment is calculated electronically. This system provides an accurate measure of central segment length in both resting and active muscle with no apparent damage to the preparation.", "contents": "Laser scanner measurement of central segment performance in isolated cardiac muscle preparations. A laser scanning system has been developed to facilitate the study of isolated cardiac muscle by excluding the possibly damaged tissue at the ends of the preparation. The laser scanner monitors the length of a central segment of the muscle that has been delineated by markers consisting of single loops of 8-0 silk suture. Focused laser light traverses the length of the muscle at 150 Hz. Scattered light is detected by a photodiode and the length of the central segment is calculated electronically. This system provides an accurate measure of central segment length in both resting and active muscle with no apparent damage to the preparation."}
{"id": "PMID:464121", "title": "In vivo measurement of blood vessel wall thickness.", "content": "To understand the mechanical properties of arteries and vascular grafts, it is crucial that the wall thickness in these vessels be known. Unfortunately, all availble methods for measuring this parameter require the removal of the vessel, which precludes the study of such vessels as a function of time. A new radiographic technique for measuring the wall thickness of arteries and vascular grafts in vivo, utilizing contrast materials injected into the vessel lumen and applied to the outer surface of the vessel, is described. Radiographs are obtained with a portable X-ray machine and analyzed using a calibrated microscope. The technique has been successfully applied to the in vivo measurement of wall thickness in canine arteries, veins, and experimental vascular grafts. It is concluded that the method provides better than 95% accuracy in a variety of vessels and that it can be used to study changes in vascular grafts after their implantation into the arterial circulation.", "contents": "In vivo measurement of blood vessel wall thickness. To understand the mechanical properties of arteries and vascular grafts, it is crucial that the wall thickness in these vessels be known. Unfortunately, all availble methods for measuring this parameter require the removal of the vessel, which precludes the study of such vessels as a function of time. A new radiographic technique for measuring the wall thickness of arteries and vascular grafts in vivo, utilizing contrast materials injected into the vessel lumen and applied to the outer surface of the vessel, is described. Radiographs are obtained with a portable X-ray machine and analyzed using a calibrated microscope. The technique has been successfully applied to the in vivo measurement of wall thickness in canine arteries, veins, and experimental vascular grafts. It is concluded that the method provides better than 95% accuracy in a variety of vessels and that it can be used to study changes in vascular grafts after their implantation into the arterial circulation."}
{"id": "PMID:464122", "title": "Evaluation of a screen oxygenator suitable for small animal organ perfusion.", "content": "A small vertical screen oxygenator was built using a stainless steel screen enclosed in a Plexiglas box as the oxygenating surface. The unit was primed with 35 ml dextran-diluted blood (Hct 25.1 +/- 0.6%. mean +/- SE) and tested in cats with a partial cardiopulmonary bypass circuit. An oxygen saturation of more than 95% was always obtained, even when the incoming venous saturation was as low as 15%. The O2 exchange capacity was minimally affected by changes in blood flow (1.5 to 70 ml/min) through the unit. CO2 extraction was not flow limited over the range tested. Output PCO2 was 33.3 +/- 0.6 mmHg and pH was 7.29 +/- 0.02. These results were obtained when 3% CO2 in O2 was passed through the oxygenator chamber at 1.0 to 5.0 l/min. The performance of the unit was stable for periods up to 4 h. The small priming volume and reliable performance make this oxygenator suitable for organ perfusion in small experimental animals.", "contents": "Evaluation of a screen oxygenator suitable for small animal organ perfusion. A small vertical screen oxygenator was built using a stainless steel screen enclosed in a Plexiglas box as the oxygenating surface. The unit was primed with 35 ml dextran-diluted blood (Hct 25.1 +/- 0.6%. mean +/- SE) and tested in cats with a partial cardiopulmonary bypass circuit. An oxygen saturation of more than 95% was always obtained, even when the incoming venous saturation was as low as 15%. The O2 exchange capacity was minimally affected by changes in blood flow (1.5 to 70 ml/min) through the unit. CO2 extraction was not flow limited over the range tested. Output PCO2 was 33.3 +/- 0.6 mmHg and pH was 7.29 +/- 0.02. These results were obtained when 3% CO2 in O2 was passed through the oxygenator chamber at 1.0 to 5.0 l/min. The performance of the unit was stable for periods up to 4 h. The small priming volume and reliable performance make this oxygenator suitable for organ perfusion in small experimental animals."}
{"id": "PMID:464123", "title": "Nonpressor mechanisms in CNS-induced natriuresis.", "content": "Ventriculocisternal perfusion was performed in pentobarbital-anesthetized dogs. Perfusion of high Na (300 mM NaCl) artificial cerebrospinal fluid (CSF) (E) for 2 h was preceded by 2 h of control (C) and was followed by 2 h of recovery (R) during which normal (150 mM NaCl) artificial CSF was perfused. A time-control group was perfused with normal artificial CSF throughout C, E, and R. High sodium perfusion resulted in a marked natriuresis in each of nine animals and suppression of plasma renin activity. Theere were no simultaneous changes in mean arterial pressure, glomerular filtration rate, or renal plasma flow. Sodium excretion and plasma renin activity showed a slight gradual rise in the time-control group, but no significant differences were observed between the C and E periods; sodium excretion and plasma renin activity were similar in the high Na and time-control groups during C and R, but significantly different during E. It is concluded that when CSF sodium is elevated by perfusing artificial CSF, the resulting natriuresis and suppression of plasma renin activity are not caused by hemodynamic changes.", "contents": "Nonpressor mechanisms in CNS-induced natriuresis. Ventriculocisternal perfusion was performed in pentobarbital-anesthetized dogs. Perfusion of high Na (300 mM NaCl) artificial cerebrospinal fluid (CSF) (E) for 2 h was preceded by 2 h of control (C) and was followed by 2 h of recovery (R) during which normal (150 mM NaCl) artificial CSF was perfused. A time-control group was perfused with normal artificial CSF throughout C, E, and R. High sodium perfusion resulted in a marked natriuresis in each of nine animals and suppression of plasma renin activity. Theere were no simultaneous changes in mean arterial pressure, glomerular filtration rate, or renal plasma flow. Sodium excretion and plasma renin activity showed a slight gradual rise in the time-control group, but no significant differences were observed between the C and E periods; sodium excretion and plasma renin activity were similar in the high Na and time-control groups during C and R, but significantly different during E. It is concluded that when CSF sodium is elevated by perfusing artificial CSF, the resulting natriuresis and suppression of plasma renin activity are not caused by hemodynamic changes."}
{"id": "PMID:464127", "title": "Denial and affirmation in illness and health.", "content": "Recent research has shown that patients with life-threatening illnesses have improved chances of survival if, rather than recognizing the serious consequences of their illness, they employ the psychological defense mechanism of denial. Reexamination of these data suggests that unexamined psychological factors which promote a positive view of health are of equal or greater importance in explaining those results. Health care professionals, concerned with diagnosis and treatment, primarily focus attention on illness and its denial or recognition. To expand this perspective to include factors for health, the author presents a four-cell paradigm of illness, health, denial, and affirmation. The paradigm is illustrated with examples of common problems in medical care.", "contents": "Denial and affirmation in illness and health. Recent research has shown that patients with life-threatening illnesses have improved chances of survival if, rather than recognizing the serious consequences of their illness, they employ the psychological defense mechanism of denial. Reexamination of these data suggests that unexamined psychological factors which promote a positive view of health are of equal or greater importance in explaining those results. Health care professionals, concerned with diagnosis and treatment, primarily focus attention on illness and its denial or recognition. To expand this perspective to include factors for health, the author presents a four-cell paradigm of illness, health, denial, and affirmation. The paradigm is illustrated with examples of common problems in medical care."}
{"id": "PMID:464128", "title": "Administrative relationships between community mental health centers and academic psychiatry departments.", "content": "The authors reviewed the administrative relationships between academic departments of psychiatry and community mental health centers (CMHCs). By telephone, they surveyed the chairpersons of 110 departments of psychiatry with approved psychiatric residency programs and found that 87 (79%) had some type of administrative relationship with a comprehensive CMHC. Almost two-thirds of the chairpersons of these departments considered the CMHC to be of major importance to their educational programs. Only 8 (7%) of the 110 departments had discontinued their relationship with a CMHC. Most of the chairpersons whose departments were involved with a center were optimistic about the future of their programs in CMHCs.", "contents": "Administrative relationships between community mental health centers and academic psychiatry departments. The authors reviewed the administrative relationships between academic departments of psychiatry and community mental health centers (CMHCs). By telephone, they surveyed the chairpersons of 110 departments of psychiatry with approved psychiatric residency programs and found that 87 (79%) had some type of administrative relationship with a comprehensive CMHC. Almost two-thirds of the chairpersons of these departments considered the CMHC to be of major importance to their educational programs. Only 8 (7%) of the 110 departments had discontinued their relationship with a CMHC. Most of the chairpersons whose departments were involved with a center were optimistic about the future of their programs in CMHCs."}
{"id": "PMID:464129", "title": "Impact of restrictive civil commitment procedures on a prison psychiatric service.", "content": "To assess the impact of restrictive civil commitment procedures, the authors studied the population of a prison psychiatric unit before and after the procedures were implemented. Their findings suggest that when the community finds it difficult to use the alternative of involuntary hospitalization, arrest and imprisonment are used to remove mentally ill people from the community.", "contents": "Impact of restrictive civil commitment procedures on a prison psychiatric service. To assess the impact of restrictive civil commitment procedures, the authors studied the population of a prison psychiatric unit before and after the procedures were implemented. Their findings suggest that when the community finds it difficult to use the alternative of involuntary hospitalization, arrest and imprisonment are used to remove mentally ill people from the community."}
{"id": "PMID:464130", "title": "Judging emotions of normal and schizophrenic subjects.", "content": "Thirty-two nursing students were shown silent films in which 10 normal and 10 schizophrenic women described a happy, sad, and an angry personal experience. Accuracy in judging the content of films of normal subjects was associated with social detachment and mechanical interests, whereas accuracy in judging the emotions of the schizophrenic subjects was associated with social involvement and nursing interests. Further, the students nurses' ability to relate to patients, as judged by their nursing supervisors, was associated with their ability to accurately identify the nonverbal emotional communications of the schizophrenic patients but not of the normal persons.", "contents": "Judging emotions of normal and schizophrenic subjects. Thirty-two nursing students were shown silent films in which 10 normal and 10 schizophrenic women described a happy, sad, and an angry personal experience. Accuracy in judging the content of films of normal subjects was associated with social detachment and mechanical interests, whereas accuracy in judging the emotions of the schizophrenic subjects was associated with social involvement and nursing interests. Further, the students nurses' ability to relate to patients, as judged by their nursing supervisors, was associated with their ability to accurately identify the nonverbal emotional communications of the schizophrenic patients but not of the normal persons."}
{"id": "PMID:464131", "title": "Decreased 24-hour urinary MHPG in childhood autism.", "content": "The authors compared a group of boys with childhood autism and a group of normal boys of similar age and found a decrease in urinary 3-methoxy-4-hydroxyphenethylene glycol (MHPG) in the autistic group. They hypothesize that autistic children might have an alteration in central and peripheral noradrenergic function, which might be related to impaired regulation of attention, arousal, and anxiety.", "contents": "Decreased 24-hour urinary MHPG in childhood autism. The authors compared a group of boys with childhood autism and a group of normal boys of similar age and found a decrease in urinary 3-methoxy-4-hydroxyphenethylene glycol (MHPG) in the autistic group. They hypothesize that autistic children might have an alteration in central and peripheral noradrenergic function, which might be related to impaired regulation of attention, arousal, and anxiety."}
{"id": "PMID:464132", "title": "A language-cultural course for foreign psychiatric residents.", "content": "Foreign medical graduates are an important component of the health care system in the United States. All foreign medical graduates have acculturation problems, but they are especially aggravating among foreign psychiatric residents. These difficulties affect the personal lives of the foreign residents as well as crucial teacher-student and doctor-patient relationships. The authors report on an effort to further define these problems in a residency program and their systematic attempts to solve them by offering a special course. The program they describe benefitted not only the foreign residents but their families and the residency program itself.", "contents": "A language-cultural course for foreign psychiatric residents. Foreign medical graduates are an important component of the health care system in the United States. All foreign medical graduates have acculturation problems, but they are especially aggravating among foreign psychiatric residents. These difficulties affect the personal lives of the foreign residents as well as crucial teacher-student and doctor-patient relationships. The authors report on an effort to further define these problems in a residency program and their systematic attempts to solve them by offering a special course. The program they describe benefitted not only the foreign residents but their families and the residency program itself."}
{"id": "PMID:464133", "title": "Amyl nitrite use among homosexual men.", "content": "The authors interviewed 150 homosexual men to determine the relationship between use of amyl nitrite and certain aspects of homosexuality. They found that the use of amyl nitrite is strongly related to a number of unconventional, deviant sexual practices and to certain medically related problems.", "contents": "Amyl nitrite use among homosexual men. The authors interviewed 150 homosexual men to determine the relationship between use of amyl nitrite and certain aspects of homosexuality. They found that the use of amyl nitrite is strongly related to a number of unconventional, deviant sexual practices and to certain medically related problems."}
{"id": "PMID:464134", "title": "Three cases of manic-depressive illness in mentally retarded adults.", "content": "The authors point out that manic-depressive illness is often not diagnosed in mentally retarded patients because they are already regarded as \"different.\" They discuss the use of lithium carbonate as a prophylactic agent in the treatment of manic-depressive illness in mentally retarded patients and present three case histories illustrating some of the difficulties encountered in diagnosing mania or hypomania in such patients. They provide a number of suggestions to help physicians recognize and treat these disorders.", "contents": "Three cases of manic-depressive illness in mentally retarded adults. The authors point out that manic-depressive illness is often not diagnosed in mentally retarded patients because they are already regarded as \"different.\" They discuss the use of lithium carbonate as a prophylactic agent in the treatment of manic-depressive illness in mentally retarded patients and present three case histories illustrating some of the difficulties encountered in diagnosing mania or hypomania in such patients. They provide a number of suggestions to help physicians recognize and treat these disorders."}
{"id": "PMID:464135", "title": "Psychoanalytic teaching in medical education.", "content": "The author sent a questionnaire to directors of medical student education in psychiatry to determine the role of psychoanalytic concepts in medical education. Practically all of the 66 respondents indicated that psychodynamic theory had been incorporated into major required courses. More than half considered the concepts of defense mechanism countertransference, transference, and dynamic unconscious necessary to general education. The author concludes that psychoanalytic principles will continue to provide a unique view into illness behavior and the doctor-patient relationship.", "contents": "Psychoanalytic teaching in medical education. The author sent a questionnaire to directors of medical student education in psychiatry to determine the role of psychoanalytic concepts in medical education. Practically all of the 66 respondents indicated that psychodynamic theory had been incorporated into major required courses. More than half considered the concepts of defense mechanism countertransference, transference, and dynamic unconscious necessary to general education. The author concludes that psychoanalytic principles will continue to provide a unique view into illness behavior and the doctor-patient relationship."}
{"id": "PMID:464136", "title": "Community mental health centers: returning to basics.", "content": "Community mental health centers (CMHCs) have made a significant contribution to making mental health services available to many Americans; in 1977 they served almost two million people. External fiscal constraints and internal service and accountability requirements now test the viability of CMHCs, but the most recent extension of the law allows increased flexibility on implementation, and the President's Commission on Mental Health has reinforced the original purpose of CMHCs by calling for an initiative to give special attention to the needs of children and youth, the elderly, minorities, the chronically mentally ill, and other underserved populations and areas.", "contents": "Community mental health centers: returning to basics. Community mental health centers (CMHCs) have made a significant contribution to making mental health services available to many Americans; in 1977 they served almost two million people. External fiscal constraints and internal service and accountability requirements now test the viability of CMHCs, but the most recent extension of the law allows increased flexibility on implementation, and the President's Commission on Mental Health has reinforced the original purpose of CMHCs by calling for an initiative to give special attention to the needs of children and youth, the elderly, minorities, the chronically mentally ill, and other underserved populations and areas."}
{"id": "PMID:464161", "title": "Piaget's stages of cognitive development and adult character structure.", "content": "Psychonalytic and Piagetian findings are bridged by understanding character structure. Social cognition of the three character types corresponds to three phase of cognitive development: Symbolic, Intuitive, and (Concrete) Operational. A child constructs his supergo by coordinating his perceptions of his caretaking as he coordinates other perceptions in the Operational Period.", "contents": "Piaget's stages of cognitive development and adult character structure. Psychonalytic and Piagetian findings are bridged by understanding character structure. Social cognition of the three character types corresponds to three phase of cognitive development: Symbolic, Intuitive, and (Concrete) Operational. A child constructs his supergo by coordinating his perceptions of his caretaking as he coordinates other perceptions in the Operational Period."}
{"id": "PMID:464162", "title": "Critique on primal therapy.", "content": "This paper attempts to indicate some of the strengths and weakenesses of primal therapy, some of the ways it is changing and how it can be incorporated within more traditional psychodynamic frames of reference.", "contents": "Critique on primal therapy. This paper attempts to indicate some of the strengths and weakenesses of primal therapy, some of the ways it is changing and how it can be incorporated within more traditional psychodynamic frames of reference."}
{"id": "PMID:464163", "title": "The \"Newsletter\": a catalyst for learning in group psychotherapy.", "content": "This article reports on an experiment in which members of a therapy group were invited to take turns doing process recordings of meetings, which were then circulated weekly to all members. The history of this \"Newsletter,\" its benefits, the therapeutic goals, and the resistance it encountered are described. The method is seen as a potentially enriching tool for group psychotherapy.", "contents": "The \"Newsletter\": a catalyst for learning in group psychotherapy. This article reports on an experiment in which members of a therapy group were invited to take turns doing process recordings of meetings, which were then circulated weekly to all members. The history of this \"Newsletter,\" its benefits, the therapeutic goals, and the resistance it encountered are described. The method is seen as a potentially enriching tool for group psychotherapy."}
{"id": "PMID:464164", "title": "Alexithymia and psychotherapy.", "content": "Alexithymia represents a disturbance in affective and cognitive function which overlaps diagnostic categories. Emotions are not differential, and are poor verbalized. Imagination related to drive fulfillment is limited. These and other problems seriously interfere with the patients' capacity to benefit from dynamic, uncovering or \"anxiety-producing\" psychotherapy. In order to consider possible remediation of the problem, we must explore the nature and causes of this disturbance.", "contents": "Alexithymia and psychotherapy. Alexithymia represents a disturbance in affective and cognitive function which overlaps diagnostic categories. Emotions are not differential, and are poor verbalized. Imagination related to drive fulfillment is limited. These and other problems seriously interfere with the patients' capacity to benefit from dynamic, uncovering or \"anxiety-producing\" psychotherapy. In order to consider possible remediation of the problem, we must explore the nature and causes of this disturbance."}
{"id": "PMID:464165", "title": "Psychotherapy of the obsessional.", "content": "Obsessional defenses require a confronting, active therapist in order to break through the intellectualized, ritualized, and highly distracting productions of the patients. The tendency to obfuscate through details and rigid adherence to the instructions demands a loose, flexible, and direct interaction from a therapist who is not too obessional and willing to forego his own perfectionistic expectations from therapy.", "contents": "Psychotherapy of the obsessional. Obsessional defenses require a confronting, active therapist in order to break through the intellectualized, ritualized, and highly distracting productions of the patients. The tendency to obfuscate through details and rigid adherence to the instructions demands a loose, flexible, and direct interaction from a therapist who is not too obessional and willing to forego his own perfectionistic expectations from therapy."}
{"id": "PMID:464166", "title": "Behavioral problems masking depression--cultural and clinical survey.", "content": "In different cultures depressions are masked by different acting-out behaviors; they vary as to type and frequency and are colored by local customs and social determinants. In Western societies hypochondriasis and psychosomatic disorders are the more common masking processes. The broad spectrum of acting-out behavioral patterns pose diagnostic and therapeutic challenges.", "contents": "Behavioral problems masking depression--cultural and clinical survey. In different cultures depressions are masked by different acting-out behaviors; they vary as to type and frequency and are colored by local customs and social determinants. In Western societies hypochondriasis and psychosomatic disorders are the more common masking processes. The broad spectrum of acting-out behavioral patterns pose diagnostic and therapeutic challenges."}
{"id": "PMID:464167", "title": "Heredity and character disorders.", "content": "The role of heredity in personality development and character disorder has been studied by a variety of means. These include investigation of twins and/or families, using clinical ratings, personality scales, or psychological tests; observation of neonates and infants; and longitudinal description of discordant pairs of twins. Attention to the interaction of heredity and environment provides new strategies for prevention and treatment.", "contents": "Heredity and character disorders. The role of heredity in personality development and character disorder has been studied by a variety of means. These include investigation of twins and/or families, using clinical ratings, personality scales, or psychological tests; observation of neonates and infants; and longitudinal description of discordant pairs of twins. Attention to the interaction of heredity and environment provides new strategies for prevention and treatment."}
{"id": "PMID:464168", "title": "Group therapy with gender-dysphoric patients.", "content": "A two-year study utilizing expressive dynamic group psychotherapy with gender-dysphoric patients is described. Two distinct stages in the emergent therapeutic relationship are labeled and clinically evaluated. Specific strategies and goals and the problems and processes of running such a therapy group are discussed at length.", "contents": "Group therapy with gender-dysphoric patients. A two-year study utilizing expressive dynamic group psychotherapy with gender-dysphoric patients is described. Two distinct stages in the emergent therapeutic relationship are labeled and clinically evaluated. Specific strategies and goals and the problems and processes of running such a therapy group are discussed at length."}
{"id": "PMID:464169", "title": "New views on the psychodynamics of phobias.", "content": "After having differentiated phobias from pseudophobias, the author describes the three stages in the psychodynamics of this psychoneurotic condition. A case is reported in detail and interpretations offered. The new hypothesis is advanced that the unconscious motivation and the defenses go beyond the individual protection of the patient. Psychotherapy is discussed.", "contents": "New views on the psychodynamics of phobias. After having differentiated phobias from pseudophobias, the author describes the three stages in the psychodynamics of this psychoneurotic condition. A case is reported in detail and interpretations offered. The new hypothesis is advanced that the unconscious motivation and the defenses go beyond the individual protection of the patient. Psychotherapy is discussed."}
{"id": "PMID:464170", "title": "Lateral capsualr ligament complex: anatomical and surgical considerations.", "content": "Acute avulsion of the bony attachment of the lateral capsular ligament was observed on x-ray films in September 1975. Seven instances were confirmed at surgery during the following 29-month period. The avulsion was reproduced in six amputation specimens which were dissected to identify the components of the lateral capsular complex. Dynamic studies were performed on a set of amputation specimens to determine the role of the various structures contributing to lateral rotary instability. The dissection revealed that the lateral capsular ligament complex has vertical and horizontal components. The dynamic studies showed that lateral pivot shift was produced by a lateral capsular release, a complete section of the anterior cruciate ligament, and a partial tibial collateral ligament release. Based on these observations, 30 patients have subsequently had reconstructive surgery to eliminate their lateral rotational instability. In the reconstruct procedure, both the bony attachment of the lateral capsular ligament and the iliotibial tract with Gerdy's tubercle have been moved anteriorly and inferiorly without separating their interconnections or any attachments. Clinical experience in these 30 patients supports the evidence obtained in the dynamic studies. Restoration of stability is secure when both the lateral capsular ligament and the iliotibial tract are advance with their anatomical connections intact.", "contents": "Lateral capsualr ligament complex: anatomical and surgical considerations. Acute avulsion of the bony attachment of the lateral capsular ligament was observed on x-ray films in September 1975. Seven instances were confirmed at surgery during the following 29-month period. The avulsion was reproduced in six amputation specimens which were dissected to identify the components of the lateral capsular complex. Dynamic studies were performed on a set of amputation specimens to determine the role of the various structures contributing to lateral rotary instability. The dissection revealed that the lateral capsular ligament complex has vertical and horizontal components. The dynamic studies showed that lateral pivot shift was produced by a lateral capsular release, a complete section of the anterior cruciate ligament, and a partial tibial collateral ligament release. Based on these observations, 30 patients have subsequently had reconstructive surgery to eliminate their lateral rotational instability. In the reconstruct procedure, both the bony attachment of the lateral capsular ligament and the iliotibial tract with Gerdy's tubercle have been moved anteriorly and inferiorly without separating their interconnections or any attachments. Clinical experience in these 30 patients supports the evidence obtained in the dynamic studies. Restoration of stability is secure when both the lateral capsular ligament and the iliotibial tract are advance with their anatomical connections intact."}
{"id": "PMID:464171", "title": "Strains of the posterior calf musculature (\"tennis leg\").", "content": "Strains of the posterior calf musculature frequently relate to overuse of the gastrocnemius muscle. The principal sports involved are the slower side-to-side sports. Treatment routine is composed of pain relief, passive stretching, exercises for the antagonists and later the agonists, and quadriceps exercises. In a series of 720 patients, over a 12-year period, this routine has been effective as evidenced by a recurrence of the condition in only 0.7% of the patients. Prevention of the injury is best obtained by regular stretching and strength development routines.", "contents": "Strains of the posterior calf musculature (\"tennis leg\"). Strains of the posterior calf musculature frequently relate to overuse of the gastrocnemius muscle. The principal sports involved are the slower side-to-side sports. Treatment routine is composed of pain relief, passive stretching, exercises for the antagonists and later the agonists, and quadriceps exercises. In a series of 720 patients, over a 12-year period, this routine has been effective as evidenced by a recurrence of the condition in only 0.7% of the patients. Prevention of the injury is best obtained by regular stretching and strength development routines."}
{"id": "PMID:464172", "title": "Primary internal fixation of carpal navicular fractures in the athlete.", "content": "Primary open reduction and internal fixation is an established procedure for treatment of carpal navicular fractures. With the use of an alignment device, the surgery can be performed on outpatients, under local anesthesia, in 30 to 45 min. The wrist is splinted in a protective device for approximately 6 weeks but no cast is necessary. Four of 13 patients in this series have been athletes. The procedure has allowed them to participate in sports during the healing period and to return to contact sports 6 to 8 weeks postoperatively without joint protection. These athletes have regained full range of motion in the wrist joint. Although the procedure is not recommended for hairline fractures which usually heal quickly or comminuted fractures of the navicular, it appears to be the treatment of choice for fractures of the carpal navicular bone at the level of the middle and proximal third of the bone or any displaced fragment with malposition in the axial or rotary plane.", "contents": "Primary internal fixation of carpal navicular fractures in the athlete. Primary open reduction and internal fixation is an established procedure for treatment of carpal navicular fractures. With the use of an alignment device, the surgery can be performed on outpatients, under local anesthesia, in 30 to 45 min. The wrist is splinted in a protective device for approximately 6 weeks but no cast is necessary. Four of 13 patients in this series have been athletes. The procedure has allowed them to participate in sports during the healing period and to return to contact sports 6 to 8 weeks postoperatively without joint protection. These athletes have regained full range of motion in the wrist joint. Although the procedure is not recommended for hairline fractures which usually heal quickly or comminuted fractures of the navicular, it appears to be the treatment of choice for fractures of the carpal navicular bone at the level of the middle and proximal third of the bone or any displaced fragment with malposition in the axial or rotary plane."}
{"id": "PMID:464174", "title": "Exposure to injury in major college football. A preliminary report of data collection to determine injury exposure rates and activity risk factors.", "content": "Five football teams from major colleges in the nation were surveyed during practices and games in the 1976 season. Data were collected on three packages of information designed as a computer program for tabulation, analysis, and storage of data. Investigators, with a thorough knowledge of football and trained in the use of the program, obtained the data from a given team during every drill, practice, and game of the season. Injury exposure rate was calculated as a ratio of injuries to minutes of exposure in 14 categories (12 specific drills, practice games, and other activities). Agility drills produced the fewest number of injuries for the amount of exposure time (exposure rate, 47,138; risk ractor, 1) and, therefore, it served as a base from which risk factors for all other categories would be calculated. No injuries were recorded in calisthenics. Practice games had the lowest injury exposure rate (1,009) and the highest risk factor (47). Analysis of data is incomplete at this time. The ultimate intent of the study is to help identify and eliminate harmful factors, establish norms, and provide a service to other schools that may desire exposure rate/risk factor data analyzed at a reasonable cost.", "contents": "Exposure to injury in major college football. A preliminary report of data collection to determine injury exposure rates and activity risk factors. Five football teams from major colleges in the nation were surveyed during practices and games in the 1976 season. Data were collected on three packages of information designed as a computer program for tabulation, analysis, and storage of data. Investigators, with a thorough knowledge of football and trained in the use of the program, obtained the data from a given team during every drill, practice, and game of the season. Injury exposure rate was calculated as a ratio of injuries to minutes of exposure in 14 categories (12 specific drills, practice games, and other activities). Agility drills produced the fewest number of injuries for the amount of exposure time (exposure rate, 47,138; risk ractor, 1) and, therefore, it served as a base from which risk factors for all other categories would be calculated. No injuries were recorded in calisthenics. Practice games had the lowest injury exposure rate (1,009) and the highest risk factor (47). Analysis of data is incomplete at this time. The ultimate intent of the study is to help identify and eliminate harmful factors, establish norms, and provide a service to other schools that may desire exposure rate/risk factor data analyzed at a reasonable cost."}
{"id": "PMID:464175", "title": "Recommendations for the screening of military personnel over 35 years of age for physical training programs.", "content": "Persons over 35 years old have an increased prevalence of risk factors. Careful medical examinations, even in a fit military population, are needed before initiating intensive physical training regimens. Risk factor profiling, which synthesizes major risk factors into a composite score, is a beneficial procedure by which to evaluate individuals. Training programs should be based on several considerations: (1) the individual's age and physical condition; (2) his body composition; (3) his risk factor profile; (4) his preference and physical ability for activity; and (5) the available facilities and climatic conditions. Exercise should enhance work capacity and serve as a preventive measure for coronary heart disease. The most efficient types of exercise to meet these ends are activities that involve the large muscles in continuous activity for 20 to 30 min daily, including warm-up and cool-down periods.", "contents": "Recommendations for the screening of military personnel over 35 years of age for physical training programs. Persons over 35 years old have an increased prevalence of risk factors. Careful medical examinations, even in a fit military population, are needed before initiating intensive physical training regimens. Risk factor profiling, which synthesizes major risk factors into a composite score, is a beneficial procedure by which to evaluate individuals. Training programs should be based on several considerations: (1) the individual's age and physical condition; (2) his body composition; (3) his risk factor profile; (4) his preference and physical ability for activity; and (5) the available facilities and climatic conditions. Exercise should enhance work capacity and serve as a preventive measure for coronary heart disease. The most efficient types of exercise to meet these ends are activities that involve the large muscles in continuous activity for 20 to 30 min daily, including warm-up and cool-down periods."}
{"id": "PMID:464173", "title": "Management of hand injuries in a professional football team. Review of 15 years of experience with one team.", "content": "Thirty-eight players from one professional football team suffered 46 major hand and wrist injuries during a 15-year period. Twenty-one of the injuries occurred in offensive players and 25 occurred in defensive players. The injuries included fractures, dislocations, fracture dislocations, and soft tissue injuries of the phalanges, metacarpals, carpals (particularly the navicular), and distal radius/ulna, including intra-articular injuries. Twelve surgical procedures were performed. Open reduction, internal fixation, and Lightcast immobilization devices (3M Company, Atlanta, Georgia) allowed the players to return to active participation with a minimum loss of practice time and virtually no loss of Sunday game availability. Early aggressive surgery for intra-articular and certain metacarpal fractures is the correct course of treatment, according to our analysis, in order to achieve the best possible functional results.", "contents": "Management of hand injuries in a professional football team. Review of 15 years of experience with one team. Thirty-eight players from one professional football team suffered 46 major hand and wrist injuries during a 15-year period. Twenty-one of the injuries occurred in offensive players and 25 occurred in defensive players. The injuries included fractures, dislocations, fracture dislocations, and soft tissue injuries of the phalanges, metacarpals, carpals (particularly the navicular), and distal radius/ulna, including intra-articular injuries. Twelve surgical procedures were performed. Open reduction, internal fixation, and Lightcast immobilization devices (3M Company, Atlanta, Georgia) allowed the players to return to active participation with a minimum loss of practice time and virtually no loss of Sunday game availability. Early aggressive surgery for intra-articular and certain metacarpal fractures is the correct course of treatment, according to our analysis, in order to achieve the best possible functional results."}
{"id": "PMID:464177", "title": "Orthopaedic injuries in athletes (ages 6 to 17). Comparison of injuries occurring in six sports.", "content": "A prospective study of orthopaedic injuries to children (ages 6 to 17) was conducted for a calendar year in the controlled environment of a military post. Data were collected on the number of participants, the hours of participation, and the number of injuries for six supervised sports (football, soccer, basketball, baseball, swimming, and gymnastics). An injury index factor was derived by a formula: (number of injuries x 10(4)) divided by [(number of participants) x (average number of hours of participation) x (number of weeks in the season of the sport)]. When the injury index factors were compared, the risk a participant has for sustaining an injury in football was twice as high (1.72) as its nearest competitors, basketball (0.88) and gymnastics (0.85). Soccer had an index factor of 0.29; baseball, 0.14; and swimming had a factor of zero. Eighty percent of all sports-related orthopaedic injuries involved the upper extremities. Lower extremity orthopaedic injuries occurred only in football and gymnastics. The evidence suggests that those persons concerned with reducing the number of injuries to the growing athlete in supervised sports should focus their attention on reducing the risk of injury to the upper extremities.", "contents": "Orthopaedic injuries in athletes (ages 6 to 17). Comparison of injuries occurring in six sports. A prospective study of orthopaedic injuries to children (ages 6 to 17) was conducted for a calendar year in the controlled environment of a military post. Data were collected on the number of participants, the hours of participation, and the number of injuries for six supervised sports (football, soccer, basketball, baseball, swimming, and gymnastics). An injury index factor was derived by a formula: (number of injuries x 10(4)) divided by [(number of participants) x (average number of hours of participation) x (number of weeks in the season of the sport)]. When the injury index factors were compared, the risk a participant has for sustaining an injury in football was twice as high (1.72) as its nearest competitors, basketball (0.88) and gymnastics (0.85). Soccer had an index factor of 0.29; baseball, 0.14; and swimming had a factor of zero. Eighty percent of all sports-related orthopaedic injuries involved the upper extremities. Lower extremity orthopaedic injuries occurred only in football and gymnastics. The evidence suggests that those persons concerned with reducing the number of injuries to the growing athlete in supervised sports should focus their attention on reducing the risk of injury to the upper extremities."}
{"id": "PMID:464178", "title": "Psychologic factors in competitive failures in athletes.", "content": "To compete successfully, one must be able to act aggressively in controlled, nondestructive, goal-oriented ways. Conflicts affecting aggressive behavior tend to interfere with competitive success. Depression, fear of success/competitive inhibition, and guilt are among the psychopathologic entities that affect the athlete's performance, whether he be a professional athlete or an amateur to whom athletic competition is a source of recreation and pleasure. It is important that all of those involved in the training and care of athletes be aware of emotional conditions that lead to injuries, both physical and psychologic, as well as those conditions that interfere with performance and effectiveness.", "contents": "Psychologic factors in competitive failures in athletes. To compete successfully, one must be able to act aggressively in controlled, nondestructive, goal-oriented ways. Conflicts affecting aggressive behavior tend to interfere with competitive success. Depression, fear of success/competitive inhibition, and guilt are among the psychopathologic entities that affect the athlete's performance, whether he be a professional athlete or an amateur to whom athletic competition is a source of recreation and pleasure. It is important that all of those involved in the training and care of athletes be aware of emotional conditions that lead to injuries, both physical and psychologic, as well as those conditions that interfere with performance and effectiveness."}
{"id": "PMID:464185", "title": "Malaria and erythrocyte glucose-6-phosphate dehydrogenase variants in West Africa.", "content": "In a clinical study, 702 Nigerian children aged 1-6 years were examined for malaria. Comparison of morbidity rates and parasitemia of patients with different glucose-6-phosphate dehydrogenase (G6PD) status provided evidence that in heterozygous females the gene for G6PD deficiency (GdA-/GdB) confers an advantage against malaria.", "contents": "Malaria and erythrocyte glucose-6-phosphate dehydrogenase variants in West Africa. In a clinical study, 702 Nigerian children aged 1-6 years were examined for malaria. Comparison of morbidity rates and parasitemia of patients with different glucose-6-phosphate dehydrogenase (G6PD) status provided evidence that in heterozygous females the gene for G6PD deficiency (GdA-/GdB) confers an advantage against malaria."}
{"id": "PMID:464186", "title": "Ovaries and adrenals in murine Schistosomiasis mansoni. I. Histopathological changes of the ovaries in acute and chronic infection.", "content": "Acute and chronic infections with schistosomiasis mansoni in mice were found to cause a reduction of the ovarian weight and atrophy of the corpus luteum cells, followed by lymphocytic and stroma cell infiltration. Finally, the corpora lutea disappeared completely. Acute schistosomiasis caused arrested development of the corpora lutea. Both acute and chronic schistosomiasis led to the formation of \"wheel cells\" in the interstitial tissue of the ovaries. A threshold level of intensity of disease was found to be necessary for these pathological changes. With less severe schistosomiasis, the morphology of the corpora lutea remained normal. The more intensive and long-lasting the infection, the greater became the atrophy of corpora lutea. The various factors which could have caused these pathological alterations are discussed in the light of available literature, and it is suggested that a pituitary hypofunction, and particularly a lack of luteinizing hormone effect, may play a role in the pathological transformation of the ovarian tissue.", "contents": "Ovaries and adrenals in murine Schistosomiasis mansoni. I. Histopathological changes of the ovaries in acute and chronic infection. Acute and chronic infections with schistosomiasis mansoni in mice were found to cause a reduction of the ovarian weight and atrophy of the corpus luteum cells, followed by lymphocytic and stroma cell infiltration. Finally, the corpora lutea disappeared completely. Acute schistosomiasis caused arrested development of the corpora lutea. Both acute and chronic schistosomiasis led to the formation of \"wheel cells\" in the interstitial tissue of the ovaries. A threshold level of intensity of disease was found to be necessary for these pathological changes. With less severe schistosomiasis, the morphology of the corpora lutea remained normal. The more intensive and long-lasting the infection, the greater became the atrophy of corpora lutea. The various factors which could have caused these pathological alterations are discussed in the light of available literature, and it is suggested that a pituitary hypofunction, and particularly a lack of luteinizing hormone effect, may play a role in the pathological transformation of the ovarian tissue."}
{"id": "PMID:464176", "title": "Physiologic performance of women compared to men. Observations of cadets at the United States Military Academy.", "content": "Observations about the physiologic factors which affected performance were made on the first women (total 119) admitted to the United States Military Academy, West Point, during their first 8-week training period. The physical requirements during 16 1/2-hr (average) days were essentially the same as required of the men (total 1,366). Results obtained from observations of the women compared to the men were tabulated in the following categories: hospital admissions (3.6 to 2.5%, injuries; 21 to 7.7%, medical conditions); stress fractures (10 to 1%); and loss of duty time (26 to 5% not capable of full duty at end of the 8 weeks). Also measurements were compared for hematocrits, body composition, major muscle groups of the upper and lower extremities, and maximum oxygen consumption. These short-term observations on women and men in an environment of equal physical training show there is a measureable difference in physical performance and that men have the advantage in accomplishing the tasks with fewer injuries or diseases and less apparent stress. Women may be capable of equal efficiency in aerobic metabolism.", "contents": "Physiologic performance of women compared to men. Observations of cadets at the United States Military Academy. Observations about the physiologic factors which affected performance were made on the first women (total 119) admitted to the United States Military Academy, West Point, during their first 8-week training period. The physical requirements during 16 1/2-hr (average) days were essentially the same as required of the men (total 1,366). Results obtained from observations of the women compared to the men were tabulated in the following categories: hospital admissions (3.6 to 2.5%, injuries; 21 to 7.7%, medical conditions); stress fractures (10 to 1%); and loss of duty time (26 to 5% not capable of full duty at end of the 8 weeks). Also measurements were compared for hematocrits, body composition, major muscle groups of the upper and lower extremities, and maximum oxygen consumption. These short-term observations on women and men in an environment of equal physical training show there is a measureable difference in physical performance and that men have the advantage in accomplishing the tasks with fewer injuries or diseases and less apparent stress. Women may be capable of equal efficiency in aerobic metabolism."}
{"id": "PMID:464187", "title": "Cholera in the Gilbert Island. I. Epidemiological features.", "content": "An explosive epidemic of cholera due to Vibrio cholerae, biotype El Tor, serotype Inaba, was centered on the coral atoll of Tarawa, Gilbert Islands. This outbreak was a unique experience in the South and Central Pacific region. The principal mode of spread during the peak of the outbreak was probably through contamination of the main water supply. Tarawa lagoon water and shellfish were found to be contaminated with V. cholerae and ingestion of raw fish and shellfish from the lagoon also served as a source of transmission. This outbreak raises the concern of Rother outbreaks of cholera occuring on remote and poorly equipped Pacific islands where the environmental circumstances are conducive to cholera spread.", "contents": "Cholera in the Gilbert Island. I. Epidemiological features. An explosive epidemic of cholera due to Vibrio cholerae, biotype El Tor, serotype Inaba, was centered on the coral atoll of Tarawa, Gilbert Islands. This outbreak was a unique experience in the South and Central Pacific region. The principal mode of spread during the peak of the outbreak was probably through contamination of the main water supply. Tarawa lagoon water and shellfish were found to be contaminated with V. cholerae and ingestion of raw fish and shellfish from the lagoon also served as a source of transmission. This outbreak raises the concern of Rother outbreaks of cholera occuring on remote and poorly equipped Pacific islands where the environmental circumstances are conducive to cholera spread."}
{"id": "PMID:464188", "title": "Cholera in the Gilbert Islands. II. Clinical and laboratory findings.", "content": "An epidemic of cholera due to Vibrio cholerae biotype El Tor occurred in 1977 on Tarawa in the Gilbert Islands. No cholera epidemic had occurred there previously and special problems were encountered in both the diagnosis and clinical management. The clinical features of the 585 hospital admissions on Tarawa during the first 64 days of the epidemic were recorded. Eight hospital deaths occurred in this period. A marked increase in cholera among malnourished Gilbertese children was noted. Simplified regimes for management were devised for the circumstances including schemes for oral and intravenous rehydration. Coconut water was used extensively in oral rehydration. Paramedical personnel were used effectively during the epidemic. Prophylactic tetracycline was used in household contacts of patients and was effective in reducing subsequent illness.", "contents": "Cholera in the Gilbert Islands. II. Clinical and laboratory findings. An epidemic of cholera due to Vibrio cholerae biotype El Tor occurred in 1977 on Tarawa in the Gilbert Islands. No cholera epidemic had occurred there previously and special problems were encountered in both the diagnosis and clinical management. The clinical features of the 585 hospital admissions on Tarawa during the first 64 days of the epidemic were recorded. Eight hospital deaths occurred in this period. A marked increase in cholera among malnourished Gilbertese children was noted. Simplified regimes for management were devised for the circumstances including schemes for oral and intravenous rehydration. Coconut water was used extensively in oral rehydration. Paramedical personnel were used effectively during the epidemic. Prophylactic tetracycline was used in household contacts of patients and was effective in reducing subsequent illness."}
{"id": "PMID:464189", "title": "Acremonium recifei as an agent of mycetoma in India.", "content": "Acremonium recifei was identified as the causal agent in a case of white grain eumycotic mycetoma of the hand which occurred in South India. The identification was based on the structure of the granules in tissue and on the morphological characteristics of the culture obtained from the granules. This case represents the first documented instance in which A. recifei has been found outside of Latin America.", "contents": "Acremonium recifei as an agent of mycetoma in India. Acremonium recifei was identified as the causal agent in a case of white grain eumycotic mycetoma of the hand which occurred in South India. The identification was based on the structure of the granules in tissue and on the morphological characteristics of the culture obtained from the granules. This case represents the first documented instance in which A. recifei has been found outside of Latin America."}
{"id": "PMID:464190", "title": "Additional isolations of Blastomyces dermatitidis from natural sites.", "content": "The ecological niche of Blastomyces dermatitidis has not been established. This paper describes the repeat isolation of this pathogenic fungus from organic debris collected at a natural site and another, previously not reported, isolation. Although the precise environmental source of this pathogen still cannot be defined, each isolation of this fungus from a natural site adds to the understanding of the ecology of this organism.", "contents": "Additional isolations of Blastomyces dermatitidis from natural sites. The ecological niche of Blastomyces dermatitidis has not been established. This paper describes the repeat isolation of this pathogenic fungus from organic debris collected at a natural site and another, previously not reported, isolation. Although the precise environmental source of this pathogen still cannot be defined, each isolation of this fungus from a natural site adds to the understanding of the ecology of this organism."}
{"id": "PMID:464191", "title": "Epidemic dengue hemorrhagic fever in rural Indonesia. I. Virological and epidemiological studies.", "content": "Virological studies were carried out during an epidemic of dengue hemorrhagic fever in Central Java, Indonesia in 1976. Dengue virus was isolated from the acute sera of 45 of 69 patients (65%). The isolation rate was higher in primary than secondary cases. Dengue 3 was the predominant serotype being transmitted (27 isolates), but both dengue 1 (8 isolates) and dengue 4 (10 isolates) were also being transmitted. A composite picture of magnitude and duration of viremia showed that many patients were circulating over 10(8) MID50 per milliliter dengue 3 virus for the first 3 days of illness and that viremia persisted for 5-6 days in some persons. If all shock cases were considered, there was no relationship between dengue serotype and severity of disease. All three confirmed fatal cases, however, were associated with dengue type 3 infections.", "contents": "Epidemic dengue hemorrhagic fever in rural Indonesia. I. Virological and epidemiological studies. Virological studies were carried out during an epidemic of dengue hemorrhagic fever in Central Java, Indonesia in 1976. Dengue virus was isolated from the acute sera of 45 of 69 patients (65%). The isolation rate was higher in primary than secondary cases. Dengue 3 was the predominant serotype being transmitted (27 isolates), but both dengue 1 (8 isolates) and dengue 4 (10 isolates) were also being transmitted. A composite picture of magnitude and duration of viremia showed that many patients were circulating over 10(8) MID50 per milliliter dengue 3 virus for the first 3 days of illness and that viremia persisted for 5-6 days in some persons. If all shock cases were considered, there was no relationship between dengue serotype and severity of disease. All three confirmed fatal cases, however, were associated with dengue type 3 infections."}
{"id": "PMID:464192", "title": "Epidemic dengue hemorrhagic fever in rural Indonesia. II. Clinical studies.", "content": "Clinical observations were made on 95 serologically or virologically confirmed dengue fever cases during an epidemic in a rural area of Indonesia in December 1976. The age distribution was similar to that observed in patients with dengue hemorrhagic fever in Jakarta, a highly endemic urban area. The observed disease ranged in severity from undifferentiated fever to shock and death. The majority of patients had acute onset of fever with nausea, vomiting, headache, and abdominal pain. Hepatomegaly was observed in only 19% of the patients. A positive tourniquet test was the most frequently observed hemorrhagic manifestation, but epistaxis was observed in 20% and hematemesis in 6% of the patients. Dengue shock syndrome was observed in 37% of the patients. There were four deaths, three of which were confirmed as due to dengue infection by virus isolation. The data suggest that one, and possibly two, of the fatal cases with virus isolation were primary infections, based on the results of hemagglutination-inhibition test using all four dengue antigens.", "contents": "Epidemic dengue hemorrhagic fever in rural Indonesia. II. Clinical studies. Clinical observations were made on 95 serologically or virologically confirmed dengue fever cases during an epidemic in a rural area of Indonesia in December 1976. The age distribution was similar to that observed in patients with dengue hemorrhagic fever in Jakarta, a highly endemic urban area. The observed disease ranged in severity from undifferentiated fever to shock and death. The majority of patients had acute onset of fever with nausea, vomiting, headache, and abdominal pain. Hepatomegaly was observed in only 19% of the patients. A positive tourniquet test was the most frequently observed hemorrhagic manifestation, but epistaxis was observed in 20% and hematemesis in 6% of the patients. Dengue shock syndrome was observed in 37% of the patients. There were four deaths, three of which were confirmed as due to dengue infection by virus isolation. The data suggest that one, and possibly two, of the fatal cases with virus isolation were primary infections, based on the results of hemagglutination-inhibition test using all four dengue antigens."}
{"id": "PMID:464193", "title": "Epidemic dengue hemorrhagic fever in rural Indonesia. III. Entomological studies.", "content": "Entomological studies were carried out during a dengue hemorrhagic fever epidemic in Central Java in December 1976. Both Aedes aegypti and Aedes albopictus were widely distributed in Bantul, but the latter species was more abundant. Comparative studies on the vector competence of the two species showed that Ae. albopictus had a higher susceptibility than Ae. aegypti to oral infection with all four dengue serotypes. The two species were equally compentent in transmitting the Bantul strain of dengue 3 virus after parenteral infection. The data suggest that Ae. albopictus could have been an important vector in this epidemic, but no direct observations were obtained to define the respective contribution of either Ae. albopictus or Ae. aegypti.", "contents": "Epidemic dengue hemorrhagic fever in rural Indonesia. III. Entomological studies. Entomological studies were carried out during a dengue hemorrhagic fever epidemic in Central Java in December 1976. Both Aedes aegypti and Aedes albopictus were widely distributed in Bantul, but the latter species was more abundant. Comparative studies on the vector competence of the two species showed that Ae. albopictus had a higher susceptibility than Ae. aegypti to oral infection with all four dengue serotypes. The two species were equally compentent in transmitting the Bantul strain of dengue 3 virus after parenteral infection. The data suggest that Ae. albopictus could have been an important vector in this epidemic, but no direct observations were obtained to define the respective contribution of either Ae. albopictus or Ae. aegypti."}
{"id": "PMID:464194", "title": "Further observations on infections of guinea pigs with Venezuelan encephalitis virus strains.", "content": "Guinea pigs from a Guatemalan colony died after subcutaneous inoculating of moderately small doses of equine-benign strains of Venezuelan encephalitis (VE) virus of hemagglutination-inhibition subtype I-E from enzootic habitats in Mexico and Guatemala. Thus these guinea pigs were unlike English short hair and inbred 13 guinea pigs, which usually survive infections with equine-benign VE strains of subtype I-E. We therefore caution others that not all strains of guinea pigs can be used to evaluate the potential equine virulence of VE viruses.", "contents": "Further observations on infections of guinea pigs with Venezuelan encephalitis virus strains. Guinea pigs from a Guatemalan colony died after subcutaneous inoculating of moderately small doses of equine-benign strains of Venezuelan encephalitis (VE) virus of hemagglutination-inhibition subtype I-E from enzootic habitats in Mexico and Guatemala. Thus these guinea pigs were unlike English short hair and inbred 13 guinea pigs, which usually survive infections with equine-benign VE strains of subtype I-E. We therefore caution others that not all strains of guinea pigs can be used to evaluate the potential equine virulence of VE viruses."}
{"id": "PMID:464195", "title": "Relationships of bunyamwera group viruses by neutralization.", "content": "A standardized serum dilution plaque reduction neutralization test was used for cross-neutralization studies of 23 strains of Bunyamwera serogroup viruses. Antigenic relationships were determined by inspection of the neutralization tests results as well as by numerical taxonomic analysis. Based on these analyses five complexes, containing 1-11 viruses, were distinguished. Little or no cross-reactivity was observed between viruses of different complexes. Three of the viruses tested were indistinguishable from prototypes and probably represent strains or varieties of those prototypes. A tentative classification scheme for the Bunyamwera group is presented.", "contents": "Relationships of bunyamwera group viruses by neutralization. A standardized serum dilution plaque reduction neutralization test was used for cross-neutralization studies of 23 strains of Bunyamwera serogroup viruses. Antigenic relationships were determined by inspection of the neutralization tests results as well as by numerical taxonomic analysis. Based on these analyses five complexes, containing 1-11 viruses, were distinguished. Little or no cross-reactivity was observed between viruses of different complexes. Three of the viruses tested were indistinguishable from prototypes and probably represent strains or varieties of those prototypes. A tentative classification scheme for the Bunyamwera group is presented."}
{"id": "PMID:464196", "title": "Inactivation of complement by Loxosceles reclusa spider venom.", "content": "Zymosan depletion of serum complement in guinea pigs rendered them highly resistant to lesion by Loxosceles reclusa spider venom. Guinea pigs deficient in C4 of the complement system are as sensitive to the venom as normal guinea pigs. The injection of 35 micrograms of whole recluse venom intradermally into guinea pigs lowered their complement level by 35.7%. Brown recluse spider venom in concentrations as slight as 0.02 micrograms protein/ml can totally inactivate one CH50 of guinea pig complement in vitro. Bee, scorpion, and other spider venoms had no influence on the hemolytic titer of complement. Fractionation of recluse spider venom by Sephadex G-200 filtration separated the complement-inactivating property of the venom into three major regions which could be distinguished on the basis of heat stability as well as size. None was neutralized by antivenom. Polyacrylamide gel electrophoresis of venom resolved the complement inactivators into five fractions. Complement inactivated by whole venom or the Sephadex fractions could be restored to hemolytic activity by supplements of fresh serum but not by heat-inactivated serum, pure C3, pure C5, or C3 and C5 in combination.", "contents": "Inactivation of complement by Loxosceles reclusa spider venom. Zymosan depletion of serum complement in guinea pigs rendered them highly resistant to lesion by Loxosceles reclusa spider venom. Guinea pigs deficient in C4 of the complement system are as sensitive to the venom as normal guinea pigs. The injection of 35 micrograms of whole recluse venom intradermally into guinea pigs lowered their complement level by 35.7%. Brown recluse spider venom in concentrations as slight as 0.02 micrograms protein/ml can totally inactivate one CH50 of guinea pig complement in vitro. Bee, scorpion, and other spider venoms had no influence on the hemolytic titer of complement. Fractionation of recluse spider venom by Sephadex G-200 filtration separated the complement-inactivating property of the venom into three major regions which could be distinguished on the basis of heat stability as well as size. None was neutralized by antivenom. Polyacrylamide gel electrophoresis of venom resolved the complement inactivators into five fractions. Complement inactivated by whole venom or the Sephadex fractions could be restored to hemolytic activity by supplements of fresh serum but not by heat-inactivated serum, pure C3, pure C5, or C3 and C5 in combination."}
{"id": "PMID:464197", "title": "Failure of metronidazole in a patient with hepatic amebic abscess.", "content": "The therapeutic problems of multiple amebic liver abscesses in a patient who failed to respond to metronidazole are described, and the importance of an early change to an alternative therapy is emphasized.", "contents": "Failure of metronidazole in a patient with hepatic amebic abscess. The therapeutic problems of multiple amebic liver abscesses in a patient who failed to respond to metronidazole are described, and the importance of an early change to an alternative therapy is emphasized."}
{"id": "PMID:464198", "title": "Is trichuriasis associated with iron deficiency anemia?", "content": "We performed quantitative fecal examinations, hemograms, and serum iron determinations on 103 first-grade children from Vieques Island, Puerto Rico, to determine whether trichuriasis was associated with iron deficiency and anemia. Although hemoglobin values tended to be slightly lower in Trichuris-infected children, there was no association between trichuriasis and serum iron or transferrin saturation values. These data demonstrate that in lightly infected children such as the population studied trichuriasis is not associated with iron deficiency anemia.", "contents": "Is trichuriasis associated with iron deficiency anemia? We performed quantitative fecal examinations, hemograms, and serum iron determinations on 103 first-grade children from Vieques Island, Puerto Rico, to determine whether trichuriasis was associated with iron deficiency and anemia. Although hemoglobin values tended to be slightly lower in Trichuris-infected children, there was no association between trichuriasis and serum iron or transferrin saturation values. These data demonstrate that in lightly infected children such as the population studied trichuriasis is not associated with iron deficiency anemia."}
{"id": "PMID:464199", "title": "Renal cortical necrosis following exposure to sap of the marking-nut tree (Semecarpus anacardium).", "content": "An 18-year-old man developed skin lesions and anuria following exposure to sap of the marking-nut tree (Semecarpus anacardium). Renal biopsy showed diffuse cortical necrosis. The mechanism of nephrotoxicity of the sap is unknown but may be due to its phenolic constituents.", "contents": "Renal cortical necrosis following exposure to sap of the marking-nut tree (Semecarpus anacardium). An 18-year-old man developed skin lesions and anuria following exposure to sap of the marking-nut tree (Semecarpus anacardium). Renal biopsy showed diffuse cortical necrosis. The mechanism of nephrotoxicity of the sap is unknown but may be due to its phenolic constituents."}
{"id": "PMID:464200", "title": "Low anterior resection with anastomosis by endorectal pull-through. Another look at Black's operation.", "content": "The type of endorectal pull-through described by Black was evaluated as an alternative method of anastomosis in 11 anterior resections in which anastomosis from above was impossible. The method is attractive from the standpoint of function because it preserves the distal rectum and leaves the anus intact. Technical points important to success are complete mobilization of the descending colon to allow the distal descending colon to be used for the pull-through and prophylactic dilatation of the early stenosis, which is characteristic of this type of anastomosis.", "contents": "Low anterior resection with anastomosis by endorectal pull-through. Another look at Black's operation. The type of endorectal pull-through described by Black was evaluated as an alternative method of anastomosis in 11 anterior resections in which anastomosis from above was impossible. The method is attractive from the standpoint of function because it preserves the distal rectum and leaves the anus intact. Technical points important to success are complete mobilization of the descending colon to allow the distal descending colon to be used for the pull-through and prophylactic dilatation of the early stenosis, which is characteristic of this type of anastomosis."}
{"id": "PMID:464204", "title": "Complications of continent ileostomy.", "content": "Immediate postoperative complications occurred in 15 per cent of 39 patients undergoing continent ileostomy, and late complications developed in 46 per cent of these patients. Age over 40 years, obesity, and Crohn's disease were related to the morbidity rate, but corticosteroid therapy was not a factor. Results of primary operations were superior to those of secondary continent ileostomies. With careful selection of patients and attention to technical detail, success in 90 per cent of initial continent ileostomy operations is a realistic goal.", "contents": "Complications of continent ileostomy. Immediate postoperative complications occurred in 15 per cent of 39 patients undergoing continent ileostomy, and late complications developed in 46 per cent of these patients. Age over 40 years, obesity, and Crohn's disease were related to the morbidity rate, but corticosteroid therapy was not a factor. Results of primary operations were superior to those of secondary continent ileostomies. With careful selection of patients and attention to technical detail, success in 90 per cent of initial continent ileostomy operations is a realistic goal."}
{"id": "PMID:464205", "title": "Human hyperthermic therapy: relation between tumor type and capacity to induce hyperthermia by radiofrequency.", "content": "There is mounting evidence that hyperthermia of 42 degrees C or greater is tumoricidal to cell cultures and to animal and human tumors. However, temperatures of 45 degrees C or greater may have greater potential therapeutic benefit. The ability to selectively achieve such highintratratumor temperatures without injury to normal tissue may be due to the relatively poor blood flow in tumors compared with that in normal tissues. While vascularity and blood flow are known to vary significantly among tumors, we found that of 52 tumors evaluated, temperatures of 42 degrees C or greater in 42 tumors (81 per cent) and of 45 degrees C or greater in 23 tumors (44 per cent) appeared to be independent of the histologic type of tumor. Therefore, therapeutic hyperthermia may be possible for most varieties of human solid cancer.", "contents": "Human hyperthermic therapy: relation between tumor type and capacity to induce hyperthermia by radiofrequency. There is mounting evidence that hyperthermia of 42 degrees C or greater is tumoricidal to cell cultures and to animal and human tumors. However, temperatures of 45 degrees C or greater may have greater potential therapeutic benefit. The ability to selectively achieve such highintratratumor temperatures without injury to normal tissue may be due to the relatively poor blood flow in tumors compared with that in normal tissues. While vascularity and blood flow are known to vary significantly among tumors, we found that of 52 tumors evaluated, temperatures of 42 degrees C or greater in 42 tumors (81 per cent) and of 45 degrees C or greater in 23 tumors (44 per cent) appeared to be independent of the histologic type of tumor. Therefore, therapeutic hyperthermia may be possible for most varieties of human solid cancer."}
{"id": "PMID:464206", "title": "Thoracic outlet syndrome.", "content": "Eleven patients with vascular sequelae of thoracic outlet syndrome were operated on at the University of California, SanFrancisco, during the past 17 years. Five patients presented with episodes of ischemia of the arm and hand secondary to microemboli released from subclavian arterial lesions produced by chronic compression at the thoracic outlet. Treatment consisted of arterial reconstruction, removal of the compressive structure, and cervical sympathectomy to relieve or lessen distal ischemia. Four of the five patients had good or excellent results; one patient required amputation of the forearm. The results were inversely proportional to the extent of distal arterial embolic occlusions present at the time of surgical treatment. Six patients presented with symptoms of chronic venous hypertension. Four of the six had subclavian venous thrombosis and were treated by transaxillary resection of the first rib to decompress the collateral veins within the costoclavicular space. All four were symptomatically improved. Two patients had venous hypertension due to extrinsic compression of the subclavian vein. One patient became asymptomatic and the other was markedly improved after resection for external compression. In this small series transaxillary resection of the first rib has resulted in symptomatic improvement in chronic venous hypertension of the arm.", "contents": "Thoracic outlet syndrome. Eleven patients with vascular sequelae of thoracic outlet syndrome were operated on at the University of California, SanFrancisco, during the past 17 years. Five patients presented with episodes of ischemia of the arm and hand secondary to microemboli released from subclavian arterial lesions produced by chronic compression at the thoracic outlet. Treatment consisted of arterial reconstruction, removal of the compressive structure, and cervical sympathectomy to relieve or lessen distal ischemia. Four of the five patients had good or excellent results; one patient required amputation of the forearm. The results were inversely proportional to the extent of distal arterial embolic occlusions present at the time of surgical treatment. Six patients presented with symptoms of chronic venous hypertension. Four of the six had subclavian venous thrombosis and were treated by transaxillary resection of the first rib to decompress the collateral veins within the costoclavicular space. All four were symptomatically improved. Two patients had venous hypertension due to extrinsic compression of the subclavian vein. One patient became asymptomatic and the other was markedly improved after resection for external compression. In this small series transaxillary resection of the first rib has resulted in symptomatic improvement in chronic venous hypertension of the arm."}
{"id": "PMID:464208", "title": "Reduction of infection rates in abdominal incisions by delayed wound closure techniques.", "content": "A low incidence of infection in abdominal wounds after contaminated, infected, and selected clean-contaminated operations was achieved after delayed wound closure of the skin and subcutaneous tissue. An effective method of delayed primary closure is described. Four days of open wound management with Xeroform gauze between the skin and subcutaneous tissue is followed on the 5th day be removal of the Xerform and skin approximation with Steri-Strips. Proper use of this technique is based upon appropriate assessment of wound contamination and infection risk factors. All contaminated and infected wounds are best managed with delayed primary closure and, when not possible, with healing by secondary intention. Delayed primary closure should be applied to clean-contaminated wounds if the patients are older than 60 years or have associated diabetes mellitus, malnutrition, or obesity.", "contents": "Reduction of infection rates in abdominal incisions by delayed wound closure techniques. A low incidence of infection in abdominal wounds after contaminated, infected, and selected clean-contaminated operations was achieved after delayed wound closure of the skin and subcutaneous tissue. An effective method of delayed primary closure is described. Four days of open wound management with Xeroform gauze between the skin and subcutaneous tissue is followed on the 5th day be removal of the Xerform and skin approximation with Steri-Strips. Proper use of this technique is based upon appropriate assessment of wound contamination and infection risk factors. All contaminated and infected wounds are best managed with delayed primary closure and, when not possible, with healing by secondary intention. Delayed primary closure should be applied to clean-contaminated wounds if the patients are older than 60 years or have associated diabetes mellitus, malnutrition, or obesity."}
{"id": "PMID:464211", "title": "Cardial myxomas: a clinical diagnostic challenge.", "content": "Our experience consists of seven intracardiac myxomas in five patients seen over an 8 year period. It includes one patient who had three primary tumors, each anatomically distinct, occurring at 4 year intervals. The initial clinical presentation included embolic phenomena, unexplained neurologic symptoms, hemodynamic obstruction, and vague systemic illness. Preoperative diagnosis was confirmed by echocardiography or angiocardiography in all cases. At operation, only two of seven tumors were found to arise from the vicinity of the fossa ovalis; the other five arose from the atrial wall or ventricular septum. Wide resection of the tumors constituted the key to successful treatment. All patients have done well for periods of follow-up ranging from 6 months to 8 years. Awareness of cardiac myxoma is the key to diagnosis; treatment should be uniformly successful in all instances once the proper diagnosis has been made. Although recurrences are rare, all patients must be followed up closely for the development of new tumors, and a thorough investigation of all chambers must be conducted in patients with suspected recurrence.", "contents": "Cardial myxomas: a clinical diagnostic challenge. Our experience consists of seven intracardiac myxomas in five patients seen over an 8 year period. It includes one patient who had three primary tumors, each anatomically distinct, occurring at 4 year intervals. The initial clinical presentation included embolic phenomena, unexplained neurologic symptoms, hemodynamic obstruction, and vague systemic illness. Preoperative diagnosis was confirmed by echocardiography or angiocardiography in all cases. At operation, only two of seven tumors were found to arise from the vicinity of the fossa ovalis; the other five arose from the atrial wall or ventricular septum. Wide resection of the tumors constituted the key to successful treatment. All patients have done well for periods of follow-up ranging from 6 months to 8 years. Awareness of cardiac myxoma is the key to diagnosis; treatment should be uniformly successful in all instances once the proper diagnosis has been made. Although recurrences are rare, all patients must be followed up closely for the development of new tumors, and a thorough investigation of all chambers must be conducted in patients with suspected recurrence."}
{"id": "PMID:464212", "title": "Effect of micropore filtration on pulmonary function after massive transfusion.", "content": "1. This study demonstrates a positive correlation between the number and size of infused microaggregates and the subsequent abnormality in pulmonary function as measured by oxygenation and dead space. 2. No such correlation between the severity of injury and the altered pulmonary function or transfusion volume was demonstrated. 3. We were unable to demonstrate an advantage to the use of a 40 mu micropore filter in preventing the adult respiratory distress syndrome (ARDS) or in improving pulmonary function in our patients. 4. One explanation for the failure to demonstrate such an advantage is the low efficiency of the filter used.", "contents": "Effect of micropore filtration on pulmonary function after massive transfusion. 1. This study demonstrates a positive correlation between the number and size of infused microaggregates and the subsequent abnormality in pulmonary function as measured by oxygenation and dead space. 2. No such correlation between the severity of injury and the altered pulmonary function or transfusion volume was demonstrated. 3. We were unable to demonstrate an advantage to the use of a 40 mu micropore filter in preventing the adult respiratory distress syndrome (ARDS) or in improving pulmonary function in our patients. 4. One explanation for the failure to demonstrate such an advantage is the low efficiency of the filter used."}
{"id": "PMID:464213", "title": "Surgical management of extensive tracheal lesions.", "content": "Resection and end-to-end anastomosis has been effective in correcting localized tracheal obstruction. This procedure can be utilized in the definitive management of extensive tracheal lesions requiring the resection of at least eight tracheal rings. Important clinical considerations are the precise preoperative assessment of the lesion, careful planning of anesthetic management, choice of the appropriate incision, avoidance of circumferential dissection, and the construction of an anastomosis free of disruptive tension.", "contents": "Surgical management of extensive tracheal lesions. Resection and end-to-end anastomosis has been effective in correcting localized tracheal obstruction. This procedure can be utilized in the definitive management of extensive tracheal lesions requiring the resection of at least eight tracheal rings. Important clinical considerations are the precise preoperative assessment of the lesion, careful planning of anesthetic management, choice of the appropriate incision, avoidance of circumferential dissection, and the construction of an anastomosis free of disruptive tension."}
{"id": "PMID:464215", "title": "Septic phlebitis: a neglected disease.", "content": "A review of 100 patients with peripheral septic phlebitis revealed that 54 per cent of the cases were due to intravenous catheters and 46 per cent were secondary to drug abuse. Eighty per cent of the involved veins were in the arm or neck. Pain was the most common symptom (83 per cent), with erythema and edema the most common physical signs (63 per cent). Eighty per cent of the causative organisms were gram-positive bacteria, usually Staphylococcus aureus (41 per cent) or Group A streptococcus (20 per cent). Complications were more common if septic phlebitis was due to intravenous therapy than drug abuse. No deaths were directly attributed to septic phlebitis. However, hospital stay after development of septic phlebitis was 14 days with a 56 per cent complication rate. The initial treatment of septic phlebitis should include prompt removal of the intravenous device, antibiotics, heat, and elevation. Because serious complications occur in a significant number of patients, operative excision of the involved vein should be performed if clinical deterioration occurs or if septicemia persists after 24 hours despite conservative therapy.", "contents": "Septic phlebitis: a neglected disease. A review of 100 patients with peripheral septic phlebitis revealed that 54 per cent of the cases were due to intravenous catheters and 46 per cent were secondary to drug abuse. Eighty per cent of the involved veins were in the arm or neck. Pain was the most common symptom (83 per cent), with erythema and edema the most common physical signs (63 per cent). Eighty per cent of the causative organisms were gram-positive bacteria, usually Staphylococcus aureus (41 per cent) or Group A streptococcus (20 per cent). Complications were more common if septic phlebitis was due to intravenous therapy than drug abuse. No deaths were directly attributed to septic phlebitis. However, hospital stay after development of septic phlebitis was 14 days with a 56 per cent complication rate. The initial treatment of septic phlebitis should include prompt removal of the intravenous device, antibiotics, heat, and elevation. Because serious complications occur in a significant number of patients, operative excision of the involved vein should be performed if clinical deterioration occurs or if septicemia persists after 24 hours despite conservative therapy."}
{"id": "PMID:464218", "title": "Reliability of Doppler ultrasound in the diagnosis of acute venous thrombosis both above and below the knee.", "content": "We have found the Doppler examination to be accurate in 92% of limbs with suspected iliofemoral thrombosis and in 86% of limbs with suspected calf vein thrombosis. Above the knee, the sensitivity was 94% and the specificity, 90%. Below the knee, the sensitivity was 91% and the specificity, 84%. In the hands of a skilled examiner, Doppler ultrasound is a reliable noninvasive technique for evaluating patients with suspected deep venous thrombosis and one on which therapy can safely be based.", "contents": "Reliability of Doppler ultrasound in the diagnosis of acute venous thrombosis both above and below the knee. We have found the Doppler examination to be accurate in 92% of limbs with suspected iliofemoral thrombosis and in 86% of limbs with suspected calf vein thrombosis. Above the knee, the sensitivity was 94% and the specificity, 90%. Below the knee, the sensitivity was 91% and the specificity, 84%. In the hands of a skilled examiner, Doppler ultrasound is a reliable noninvasive technique for evaluating patients with suspected deep venous thrombosis and one on which therapy can safely be based."}
{"id": "PMID:464220", "title": "Asymptomatic carotid stenosis. Immediate and long-term results after prophylactic endarterectomy.", "content": "1. A review of the immediate and long-term results of prophylactic carotid endarterectomy for asymptomatic lesions of 78 carotid arteries in 72 patients between 1961 and 1976 is presented. 2. The inhospital operative mortality was zero. Two patients experienced postoperative transient neurologic deficit with complete recovery (2.6 per cent). There were no postoperative strokes. 3. Late follow-up data demonstrated that in only one patient did a stroke subsequently develop appropriate to the operative side, and it occurred 4 years after operation. Life table analysis for neurologic events carried out for up to 15 years indicated a 96 per cent stroke-free status of the surviving patients. 4. A 42 month survival rate of 83 per cent in patients treated by prophylactic carotid endarterectomy represented a statistically significant improvement over the 67 per cent survival of a comparable group of patients reported on in the literature. 5. Prophylactic carotid endarterectomy in the experience of vascular surgeons who can offer a low operative morbidity and mortality appears to be reasonable therapy in preventing stroke and prolonging survival until a randomized controlled study comparing surgery with the natural history of untreated patients shows evidence to the contrary.", "contents": "Asymptomatic carotid stenosis. Immediate and long-term results after prophylactic endarterectomy. 1. A review of the immediate and long-term results of prophylactic carotid endarterectomy for asymptomatic lesions of 78 carotid arteries in 72 patients between 1961 and 1976 is presented. 2. The inhospital operative mortality was zero. Two patients experienced postoperative transient neurologic deficit with complete recovery (2.6 per cent). There were no postoperative strokes. 3. Late follow-up data demonstrated that in only one patient did a stroke subsequently develop appropriate to the operative side, and it occurred 4 years after operation. Life table analysis for neurologic events carried out for up to 15 years indicated a 96 per cent stroke-free status of the surviving patients. 4. A 42 month survival rate of 83 per cent in patients treated by prophylactic carotid endarterectomy represented a statistically significant improvement over the 67 per cent survival of a comparable group of patients reported on in the literature. 5. Prophylactic carotid endarterectomy in the experience of vascular surgeons who can offer a low operative morbidity and mortality appears to be reasonable therapy in preventing stroke and prolonging survival until a randomized controlled study comparing surgery with the natural history of untreated patients shows evidence to the contrary."}
{"id": "PMID:464222", "title": "Human umbilical cord vein allograft arteriovenous fistula for chemotherapy access.", "content": "Seven patients who received chronic chemotherapy and required vascular access underwent construction of an arteriovenous fistula of the leg using gluteraldehyde-tanned human umbilical cord vein allografts. No wound or graft complications have occurred and patient tolerance of the operative procedure and subsequent use of the fisulas for chemotherapy has been excellent. Our preliminary results suggest that the use of umbilical vein allograft arteriovenous fistulas is quite satisfactory for long-term chemotherapy access.", "contents": "Human umbilical cord vein allograft arteriovenous fistula for chemotherapy access. Seven patients who received chronic chemotherapy and required vascular access underwent construction of an arteriovenous fistula of the leg using gluteraldehyde-tanned human umbilical cord vein allografts. No wound or graft complications have occurred and patient tolerance of the operative procedure and subsequent use of the fisulas for chemotherapy has been excellent. Our preliminary results suggest that the use of umbilical vein allograft arteriovenous fistulas is quite satisfactory for long-term chemotherapy access."}
{"id": "PMID:464227", "title": "Division of the left renal vein. Guidelines and consequences.", "content": "Ligation and division of the left renal vein is a reasonable safe procedure in selected patients when exposure of the perirenal aorta is crucial. This manipulation is possible because of extensive venous collateralization from the left kidney in man. Measurement of the venous stump pressure before ligation is recommended to assess the degree of collateralization, and the upper limit within which the vein may be divided safely is probably in the neighborhood of 60 cm of water. Reanastomosis of the vein is not necessary for preservation of renal function, although transient left renal dysfunction may occur. Examination of the urine and careful monitoring of renal function should be routine in the postoperative period. Intravenous urography and left spermatic venography later in the postoperative course can indicate the ultimate degree of function of the left kidney and the pathways of venous collateralization. Preservation of normal function and venous architecture at the renal hilum should be the rule.", "contents": "Division of the left renal vein. Guidelines and consequences. Ligation and division of the left renal vein is a reasonable safe procedure in selected patients when exposure of the perirenal aorta is crucial. This manipulation is possible because of extensive venous collateralization from the left kidney in man. Measurement of the venous stump pressure before ligation is recommended to assess the degree of collateralization, and the upper limit within which the vein may be divided safely is probably in the neighborhood of 60 cm of water. Reanastomosis of the vein is not necessary for preservation of renal function, although transient left renal dysfunction may occur. Examination of the urine and careful monitoring of renal function should be routine in the postoperative period. Intravenous urography and left spermatic venography later in the postoperative course can indicate the ultimate degree of function of the left kidney and the pathways of venous collateralization. Preservation of normal function and venous architecture at the renal hilum should be the rule."}
{"id": "PMID:464228", "title": "Surgical treatment of chronic visceral ischemia.", "content": "Our experience with the operative repair for chronic visceral ischemia in eight patients, including two patients without visceral ischemic symptoms, is described. We prefer revascularization of as many vessels as possible and have used a small diameter Dacron bifurcation graft directed cephalad from the infrarenal aorta to the superior mesenteric artery and hepatic or splenic artery in four patients. One patient died postoperatively of an arrhythmia. Three patients died of unrelated causes 7 to 36 months after surgery. Four patients are alive 8 to 48 months after surgery. All patients were relieved of the intestinal ischemic symptoms by surgery.", "contents": "Surgical treatment of chronic visceral ischemia. Our experience with the operative repair for chronic visceral ischemia in eight patients, including two patients without visceral ischemic symptoms, is described. We prefer revascularization of as many vessels as possible and have used a small diameter Dacron bifurcation graft directed cephalad from the infrarenal aorta to the superior mesenteric artery and hepatic or splenic artery in four patients. One patient died postoperatively of an arrhythmia. Three patients died of unrelated causes 7 to 36 months after surgery. Four patients are alive 8 to 48 months after surgery. All patients were relieved of the intestinal ischemic symptoms by surgery."}
{"id": "PMID:464229", "title": "Vertebral artery trauma.", "content": "Injury to the vertebral artery is infrequent and poses special problems in exposure if it occurs above the entrance to the transverse foramen. The patients may present with neurologic symptoms. Pulsatile tinnitus and a bruit in the cervical-paravertebral region are constant findings. Permanent occlusion by a balloon catheter offers an unhurried and facile means of control after otherwise life-threatening lesions are stabilized.", "contents": "Vertebral artery trauma. Injury to the vertebral artery is infrequent and poses special problems in exposure if it occurs above the entrance to the transverse foramen. The patients may present with neurologic symptoms. Pulsatile tinnitus and a bruit in the cervical-paravertebral region are constant findings. Permanent occlusion by a balloon catheter offers an unhurried and facile means of control after otherwise life-threatening lesions are stabilized."}
{"id": "PMID:464230", "title": "Traumatic injuries of the abdominal aorta.", "content": "Despite immediate operation, patients with abdominal aortic injuries and profound hypovolemic shock do not respond to the usual methods of resuscitation and die soon after celiotomy, prior to control and repair of the aortic injury. The rate of aortic hemorrhage exceeds the ability to restore blood volume. Shock becomes \"irreversible.\" In such patients tamponade of the aortic injury may be effected by the use of an external counterpressure device such as a G-suit or MAST suit. These devices, used in conjunction with transthoracic aortic occlusion, may raise blood pressure sufficiently to perfuse the sritical coronary and cerebral circulation, allowing time to correct acidosis and locate, control, and repair the aortic injury. Early aggressive therapy should result in increased survival.", "contents": "Traumatic injuries of the abdominal aorta. Despite immediate operation, patients with abdominal aortic injuries and profound hypovolemic shock do not respond to the usual methods of resuscitation and die soon after celiotomy, prior to control and repair of the aortic injury. The rate of aortic hemorrhage exceeds the ability to restore blood volume. Shock becomes \"irreversible.\" In such patients tamponade of the aortic injury may be effected by the use of an external counterpressure device such as a G-suit or MAST suit. These devices, used in conjunction with transthoracic aortic occlusion, may raise blood pressure sufficiently to perfuse the sritical coronary and cerebral circulation, allowing time to correct acidosis and locate, control, and repair the aortic injury. Early aggressive therapy should result in increased survival."}
{"id": "PMID:464231", "title": "Role of the vascular diagnostic laboratory in the evaluation of male impotence.", "content": "Penile systolic pressures, penile volume waveforms, and postischemic reactive hyperemia were measured in 106 patients (38 potent and 68 impotent). With such testing the vascular laboratory can clearly identify those impotent patients in whom penile blood flow is normal and who would not benefit from direct arterial surgery. Unfortunately, abnormal results give no assurance that ischemia is responsible for a given patient's impotence and a multimodal approach is necessary to further evaluate such patients.", "contents": "Role of the vascular diagnostic laboratory in the evaluation of male impotence. Penile systolic pressures, penile volume waveforms, and postischemic reactive hyperemia were measured in 106 patients (38 potent and 68 impotent). With such testing the vascular laboratory can clearly identify those impotent patients in whom penile blood flow is normal and who would not benefit from direct arterial surgery. Unfortunately, abnormal results give no assurance that ischemia is responsible for a given patient's impotence and a multimodal approach is necessary to further evaluate such patients."}
{"id": "PMID:464232", "title": "A new method of preclotting fabric prostheses. A preliminary report.", "content": "A new method of preclotting arterial fabric prostheses is described. The preclotting process depends on infusion of blood into a polyethylene bag containing the prosthesis. Massage of the bag aids permeation of the graft, and a second infusion of blood over a 1 minute period seals the fraft completely. Rapid preclotting and asepsis are the unique features of this method.", "contents": "A new method of preclotting fabric prostheses. A preliminary report. A new method of preclotting arterial fabric prostheses is described. The preclotting process depends on infusion of blood into a polyethylene bag containing the prosthesis. Massage of the bag aids permeation of the graft, and a second infusion of blood over a 1 minute period seals the fraft completely. Rapid preclotting and asepsis are the unique features of this method."}
{"id": "PMID:464233", "title": "Management of acute aortic occlusion.", "content": "Acute aortic occlusion is most often seen in elderly patients with advanced cardiac disease. The management of these patients has been facilitated by the use of extraanatomic bypass. Over the past 2 years, six patients aged 55 to 87 years presented to our medical center with acute aortic occlusion, three after major operative procedures. One patient had a thrombosed abdominal aortic aneurysm; in the other five patients differentiation between saddle embolus and thrombosis of the distal aorta was impossible. There was one operative death. Four of the other five patients underwent axillobifemoral bypass and one underwent aortofemoral thrombectomy. All survived, and none required amputation. Two of the three patients who underwent preoperative aortography developed transient renal failure postoperatively. Aortography is of little value in diagnosis and is probably contraindicated in acute aortic occlusion. Our recommendation for operative management includes (1) preparation of the patient for possible axillobifemoral bypass, (2) angiography of distal runoff via both femoral arteries, (3) attempt at bilateral aortofemoral embolectomy with Fogarty catheters, and (4) axillobifemoral bypass if embolectomy fails to restore normal pulsatile flow.", "contents": "Management of acute aortic occlusion. Acute aortic occlusion is most often seen in elderly patients with advanced cardiac disease. The management of these patients has been facilitated by the use of extraanatomic bypass. Over the past 2 years, six patients aged 55 to 87 years presented to our medical center with acute aortic occlusion, three after major operative procedures. One patient had a thrombosed abdominal aortic aneurysm; in the other five patients differentiation between saddle embolus and thrombosis of the distal aorta was impossible. There was one operative death. Four of the other five patients underwent axillobifemoral bypass and one underwent aortofemoral thrombectomy. All survived, and none required amputation. Two of the three patients who underwent preoperative aortography developed transient renal failure postoperatively. Aortography is of little value in diagnosis and is probably contraindicated in acute aortic occlusion. Our recommendation for operative management includes (1) preparation of the patient for possible axillobifemoral bypass, (2) angiography of distal runoff via both femoral arteries, (3) attempt at bilateral aortofemoral embolectomy with Fogarty catheters, and (4) axillobifemoral bypass if embolectomy fails to restore normal pulsatile flow."}
{"id": "PMID:464234", "title": "Incidental splenectomy: early and late postoperative complications.", "content": "Of 542 splenectomies performed from 1964 to 1977, 242 were incidental to gastrointestinal surgery. The highest rate of complications was observed in these patients as opposed to those who underwent splenectomy for trauma or Hodgkin's disease. Eighteen laboratory studies did not show significant changes 1.5 to 10 years postoperatively in 96 patients. Ninety-three patients questioned because of late symptoms had the highest rate of complaints after incidental splenectomy.", "contents": "Incidental splenectomy: early and late postoperative complications. Of 542 splenectomies performed from 1964 to 1977, 242 were incidental to gastrointestinal surgery. The highest rate of complications was observed in these patients as opposed to those who underwent splenectomy for trauma or Hodgkin's disease. Eighteen laboratory studies did not show significant changes 1.5 to 10 years postoperatively in 96 patients. Ninety-three patients questioned because of late symptoms had the highest rate of complaints after incidental splenectomy."}
{"id": "PMID:464235", "title": "Biliary enteric fistulas. Management of 47 cases in native Americans.", "content": "The management of 47 patients with spontaneous biliary fistulas is reported. Thirteen did not have surgery, and another 8, although operated on, did not have the fistulas closed. In none of these patients have recurrent biliary symptoms developed. The 26 patients who did have surgical closure of the fistula were operated on, in nearly every instance, for symptoms of residual cholelithiasis or choledocholithiasis, and in only 4 of these patients was the fistula known to exist preoperatively.", "contents": "Biliary enteric fistulas. Management of 47 cases in native Americans. The management of 47 patients with spontaneous biliary fistulas is reported. Thirteen did not have surgery, and another 8, although operated on, did not have the fistulas closed. In none of these patients have recurrent biliary symptoms developed. The 26 patients who did have surgical closure of the fistula were operated on, in nearly every instance, for symptoms of residual cholelithiasis or choledocholithiasis, and in only 4 of these patients was the fistula known to exist preoperatively."}
{"id": "PMID:464236", "title": "Clinical significance of umbilicoportal manometry.", "content": "Ninety-six adult patients had measurements of portal pressur over 1 to 5 days via indwelling extraperitoneal umbilicoportal catheters. Liver biopsy specimens obtained during catheterization showed 50 patients to have a normal liver; the remainder had liver disease. Portal pressure averaged 16.08 +/- 2.87 (SED) cm of saline in normal patients. Ten patients, 6 with cirrhotic and 4 with normal livers, were recatheterized 1.5 days to 7 months later when they came to laparotomy. In all but one, portal pressures were decreased during anesthesia and laparotomy. Twenty additional patients undergoing cholecystectomy had portal pressures measured before and after laparotomy, and after recovery from anesthesia for 1 to 3 postoperative days. Laparotomy caused a decrease in portal pressure of 2.7 +/- 1.3 (SED) cm of saline; after recovery from anesthesia portal pressure was higher by 2.5 +/- 1.7 (SED) cm of saline. Umbilicoportal catheterization is a safe and accurate technique for studying the portal system. In the intact unanesthetized state, we consider 22 cm of saline to be the upper limit of normal for portal pressure.", "contents": "Clinical significance of umbilicoportal manometry. Ninety-six adult patients had measurements of portal pressur over 1 to 5 days via indwelling extraperitoneal umbilicoportal catheters. Liver biopsy specimens obtained during catheterization showed 50 patients to have a normal liver; the remainder had liver disease. Portal pressure averaged 16.08 +/- 2.87 (SED) cm of saline in normal patients. Ten patients, 6 with cirrhotic and 4 with normal livers, were recatheterized 1.5 days to 7 months later when they came to laparotomy. In all but one, portal pressures were decreased during anesthesia and laparotomy. Twenty additional patients undergoing cholecystectomy had portal pressures measured before and after laparotomy, and after recovery from anesthesia for 1 to 3 postoperative days. Laparotomy caused a decrease in portal pressure of 2.7 +/- 1.3 (SED) cm of saline; after recovery from anesthesia portal pressure was higher by 2.5 +/- 1.7 (SED) cm of saline. Umbilicoportal catheterization is a safe and accurate technique for studying the portal system. In the intact unanesthetized state, we consider 22 cm of saline to be the upper limit of normal for portal pressure."}
{"id": "PMID:464237", "title": "Evaluation and management of penetrating wounds of the neck: the role of emergency angiography.", "content": "With the use of emergency angiography, careful observation, and monitoring, 40 consecutive patients with penetrating wounds were selectively managed. Eleven patients were operated on with one negative exploration and one death. Twenty-nine patients were observed after negative angiography without operation on the neck and were subsequently discharged without' overlooking a significant vascular or visceral injury or amy complication. We believe this policy to be safe and effective. It should reduce the number, the morbidity, and the cost of needless mandatory surgical explorations and should guard against clinically undetected serious injuries. Operations should be reserved for those patients with clinically obvious servere vascular or visceral injury and for those with radiographically demonstrated significant lesions.", "contents": "Evaluation and management of penetrating wounds of the neck: the role of emergency angiography. With the use of emergency angiography, careful observation, and monitoring, 40 consecutive patients with penetrating wounds were selectively managed. Eleven patients were operated on with one negative exploration and one death. Twenty-nine patients were observed after negative angiography without operation on the neck and were subsequently discharged without' overlooking a significant vascular or visceral injury or amy complication. We believe this policy to be safe and effective. It should reduce the number, the morbidity, and the cost of needless mandatory surgical explorations and should guard against clinically undetected serious injuries. Operations should be reserved for those patients with clinically obvious servere vascular or visceral injury and for those with radiographically demonstrated significant lesions."}
{"id": "PMID:464238", "title": "Selective management of gunshot wounds to the neck. Report of a series and review of the literature.", "content": "Thirty-two patients with gunshot wounds to the neck, 13 with multiple pellet injuries and 19 with single missile injuries, were managed selectively. Although 3 of the 32 patients died in the hospital, no death was attributable to the neck injury. This experience and a review of the literature support the concept of selective management of penetrating neck injuries with intervention based on specific indications.", "contents": "Selective management of gunshot wounds to the neck. Report of a series and review of the literature. Thirty-two patients with gunshot wounds to the neck, 13 with multiple pellet injuries and 19 with single missile injuries, were managed selectively. Although 3 of the 32 patients died in the hospital, no death was attributable to the neck injury. This experience and a review of the literature support the concept of selective management of penetrating neck injuries with intervention based on specific indications."}
{"id": "PMID:464240", "title": "Postlactational tumoral granulomatous mastitis: a localized immune phenomenon.", "content": "Recurrent, nodular necrotizing granulomatous mastitis in two patients is reported in detail. Pathologic, immunologic, and bacteriologic studies in one patient indicate that this entity is different from plasmas cell mastitis, fat necrosis, mammary ductal ectasia, and the usual infectious and puerperal mastitis. Systemic antibreast autoimmune mastitis and vasculitis are also ruled out. Its pathogenesis, differential diagnosis, surgical treatment, and prognosis are discussed.", "contents": "Postlactational tumoral granulomatous mastitis: a localized immune phenomenon. Recurrent, nodular necrotizing granulomatous mastitis in two patients is reported in detail. Pathologic, immunologic, and bacteriologic studies in one patient indicate that this entity is different from plasmas cell mastitis, fat necrosis, mammary ductal ectasia, and the usual infectious and puerperal mastitis. Systemic antibreast autoimmune mastitis and vasculitis are also ruled out. Its pathogenesis, differential diagnosis, surgical treatment, and prognosis are discussed."}
{"id": "PMID:464242", "title": "Technique for thrombectomy of reversed saphenous vein arterial bypass grafts.", "content": "A technique for thrombectomy of saphenous vein arterial bypass grafts is described. The technique includes visual inspection of both anastomoses and avoids unnecessary trauma to the graft secondary to repeated attempts at passing a balloon catheter against the direction of the venous valves.", "contents": "Technique for thrombectomy of reversed saphenous vein arterial bypass grafts. A technique for thrombectomy of saphenous vein arterial bypass grafts is described. The technique includes visual inspection of both anastomoses and avoids unnecessary trauma to the graft secondary to repeated attempts at passing a balloon catheter against the direction of the venous valves."}
{"id": "PMID:464243", "title": "A simple method for atraumatic control of small vessels.", "content": "The use of a surgical clip to maintain tension on an elastic band provides gentle temporary occlusion without special instruments or equipment.", "contents": "A simple method for atraumatic control of small vessels. The use of a surgical clip to maintain tension on an elastic band provides gentle temporary occlusion without special instruments or equipment."}
{"id": "PMID:464250", "title": "[The therapeutic use of streptokinase in the treatment of shock lung (author's transl)].", "content": "After protracted haemorrhagic or traumatic shock, the danger of severe respiratory insufficiency is often very imminent. This serious complication is frequently acutely progressive. Even the early use of adaequate artificial respiration cannot always prevent the developement of shock lung. The significance of the different pathogenic factors is still not definitely clear. The main changes that are most imminent during shock are the organisation and carnification of cells and plasma proteins within the lung parenchyma due to increased extravasation. On the basis of pathophysiological considerations however, it seems reasonalbe to interrupt these reactions through early use of monophasic proteo- and fibrinolysis. The indication for and prognosis of a monophasic streptokinase activated fibrinolytic and proteolytic therapy was first examined in 18 patients with a clinically manifest shock lung. In 11 patients the severe initial pulmonary changes could be reversed within 48-60 hours after the beginning of treatment. The normalization of lung function was confirmed clinically and by means of x-ray and blood gas analysis. In 7 patients in which acute renal failure had occurred the progression of pulmonary failure could not be interrupted. The present study demonstrates for the first time the possibilities of fibrinolytic treatment of the shock lung. The risk of haemorrhagic complications especially in the immediate postoperative or post traumatic phase could be limited by a high dose streptokinase therapy. Under these condition the formation of excessive amounts of free plasmin in the circulation was prevented and the so-called endogenous lysis was primarily achieved.", "contents": "[The therapeutic use of streptokinase in the treatment of shock lung (author's transl)]. After protracted haemorrhagic or traumatic shock, the danger of severe respiratory insufficiency is often very imminent. This serious complication is frequently acutely progressive. Even the early use of adaequate artificial respiration cannot always prevent the developement of shock lung. The significance of the different pathogenic factors is still not definitely clear. The main changes that are most imminent during shock are the organisation and carnification of cells and plasma proteins within the lung parenchyma due to increased extravasation. On the basis of pathophysiological considerations however, it seems reasonalbe to interrupt these reactions through early use of monophasic proteo- and fibrinolysis. The indication for and prognosis of a monophasic streptokinase activated fibrinolytic and proteolytic therapy was first examined in 18 patients with a clinically manifest shock lung. In 11 patients the severe initial pulmonary changes could be reversed within 48-60 hours after the beginning of treatment. The normalization of lung function was confirmed clinically and by means of x-ray and blood gas analysis. In 7 patients in which acute renal failure had occurred the progression of pulmonary failure could not be interrupted. The present study demonstrates for the first time the possibilities of fibrinolytic treatment of the shock lung. The risk of haemorrhagic complications especially in the immediate postoperative or post traumatic phase could be limited by a high dose streptokinase therapy. Under these condition the formation of excessive amounts of free plasmin in the circulation was prevented and the so-called endogenous lysis was primarily achieved."}
{"id": "PMID:464251", "title": "[Changes of intraocular pressure in healthy-eyed patients under neuroleptanalgesia in abdominal surgery (author's transl)].", "content": "Ocular pressure was measured in 60 patients under neuroleptanalgesia with three different modes of induction (N2O, ketamine, thiopentone), during abdominal operations lasting at least one hour. The effect of premedication, of three different induction agents and of decurarisation on intraocular pressure were tested. It became apparent that after premedication and during the induction stage a decline of intraocular pressure (IOP) occured but did not last longer than 60 minutes. Decurarisation had no influence on the IOP. The causes of these changes and discussed.", "contents": "[Changes of intraocular pressure in healthy-eyed patients under neuroleptanalgesia in abdominal surgery (author's transl)]. Ocular pressure was measured in 60 patients under neuroleptanalgesia with three different modes of induction (N2O, ketamine, thiopentone), during abdominal operations lasting at least one hour. The effect of premedication, of three different induction agents and of decurarisation on intraocular pressure were tested. It became apparent that after premedication and during the induction stage a decline of intraocular pressure (IOP) occured but did not last longer than 60 minutes. Decurarisation had no influence on the IOP. The causes of these changes and discussed."}
{"id": "PMID:464252", "title": "[Heparin assay in plasma. Comparison of different methods (author's transl)].", "content": "Four different heparin assay methods were compared. Only procedures which can be routinely carried out in clinical laboratories were taken into consideration. A range of heparin concentrations was chosen for these tests which is likely to be expected in low dose heparin prophylaxis. The lowest sensitivity was found when the thrombin clotting time was applied, and slightly better results were obtained by the aPTT method. Addition of purified antithrombin III to the reaction mixtures improved the sensitivity of both methods. When factor Xa was used in a clotting test, the results were comparable to the aPTT method. The sensitivity was however, about 10 times higher when the remaining factor Xa was directly measured in a photometric assay system with a chromogenic substrate. The advantages and disadvantages of the different methods and the usefulness of heparin assays for clinical purposes are discussed.", "contents": "[Heparin assay in plasma. Comparison of different methods (author's transl)]. Four different heparin assay methods were compared. Only procedures which can be routinely carried out in clinical laboratories were taken into consideration. A range of heparin concentrations was chosen for these tests which is likely to be expected in low dose heparin prophylaxis. The lowest sensitivity was found when the thrombin clotting time was applied, and slightly better results were obtained by the aPTT method. Addition of purified antithrombin III to the reaction mixtures improved the sensitivity of both methods. When factor Xa was used in a clotting test, the results were comparable to the aPTT method. The sensitivity was however, about 10 times higher when the remaining factor Xa was directly measured in a photometric assay system with a chromogenic substrate. The advantages and disadvantages of the different methods and the usefulness of heparin assays for clinical purposes are discussed."}
{"id": "PMID:464319", "title": "Evidence for an input to the molecular layer and the stratum granulosum of the dentate gyrus from the supramammillary region of the hypothalamus.", "content": "Injections of a mixture of tritiated amino acids were made into the posterior hypothalamus in a series of rats and cats. In every case in which the injection involved a significant proportion of the cells in the supramammillary region, labeled fibers could be followed to the dentate gyrus, the anterior hippocampal rudiment and the induseum griseum of both sides. In the dentate gyrus the hypothalamic afferents terminate in a narrow band in the outer half of the stratum gramulosum and the inner 20 micron or so, of the stratum moleculare, immediately deep to the zone of termination of the associational and commissural afferents. As judged by silver grain counts across the width of the zone of labeled terminals, the projection to the ipsilateral side is several times as heavy as that to the contralateral side, and although it involves the entire septo-temporal (=rostro-caudal) extent of the gyrus on both sides, the projection to the suprapyramidal (inner) blade of the dentate gyrus is approximately twice as heavy as that to the infrapyramidal (outer) blade.", "contents": "Evidence for an input to the molecular layer and the stratum granulosum of the dentate gyrus from the supramammillary region of the hypothalamus. Injections of a mixture of tritiated amino acids were made into the posterior hypothalamus in a series of rats and cats. In every case in which the injection involved a significant proportion of the cells in the supramammillary region, labeled fibers could be followed to the dentate gyrus, the anterior hippocampal rudiment and the induseum griseum of both sides. In the dentate gyrus the hypothalamic afferents terminate in a narrow band in the outer half of the stratum gramulosum and the inner 20 micron or so, of the stratum moleculare, immediately deep to the zone of termination of the associational and commissural afferents. As judged by silver grain counts across the width of the zone of labeled terminals, the projection to the ipsilateral side is several times as heavy as that to the contralateral side, and although it involves the entire septo-temporal (=rostro-caudal) extent of the gyrus on both sides, the projection to the suprapyramidal (inner) blade of the dentate gyrus is approximately twice as heavy as that to the infrapyramidal (outer) blade."}
{"id": "PMID:464320", "title": "Catecholamines in the yolk sac epithelium of the rat.", "content": "Catecholamines were found histochemically in the visceral yolk sac of the rat from embryonic day (ED) 10, i.e. before the amines become detectable in peripheral or central neurons of the fetus. Formaldehyde-induced fluorescence was confined to the apical part of the yolk sac epithelial cells. The specificity of histofluorescence has been confirmed by borohydride reduction, microspectrofluorimetry revealing an emission peak at 480 nm and administration of reserpine. The catecholamines present were identified by mass fragmentography using N,O-trifluoroacetyl derivatives. At ED 13 both dopamine and norepinephrine were present, while only dopamine was detected at ED 18 1/2. Maternal circulation or the epithelial cells themselves appear as possible sources of these catecholamines. The occurrence of amines in the yolk sac epithelium may reflect an intracellular role of these compounds, a barrier function of the epithelium or a step in a transport to the fetus where the amines might assume regulatory functions.", "contents": "Catecholamines in the yolk sac epithelium of the rat. Catecholamines were found histochemically in the visceral yolk sac of the rat from embryonic day (ED) 10, i.e. before the amines become detectable in peripheral or central neurons of the fetus. Formaldehyde-induced fluorescence was confined to the apical part of the yolk sac epithelial cells. The specificity of histofluorescence has been confirmed by borohydride reduction, microspectrofluorimetry revealing an emission peak at 480 nm and administration of reserpine. The catecholamines present were identified by mass fragmentography using N,O-trifluoroacetyl derivatives. At ED 13 both dopamine and norepinephrine were present, while only dopamine was detected at ED 18 1/2. Maternal circulation or the epithelial cells themselves appear as possible sources of these catecholamines. The occurrence of amines in the yolk sac epithelium may reflect an intracellular role of these compounds, a barrier function of the epithelium or a step in a transport to the fetus where the amines might assume regulatory functions."}
{"id": "PMID:464321", "title": "Taste buds in vallate papillae grafted to the anterior chamber of the eye.", "content": "Vallate papillae from rats were grafted to the anterior chamber of the eye of the same animal. Longterm grafts showed small taste buds located in epitelial cavities which were probably the remnants of trench walls. These grafts have been reinnervated by local nerves. Excretory ducts and serous acini of Von Ebner's glands were also present. The latter showed secretory granules in their cells. The specificity of the nerve fibers and of the epithelium in relation to the induction of taste bud differentiation are discussed.", "contents": "Taste buds in vallate papillae grafted to the anterior chamber of the eye. Vallate papillae from rats were grafted to the anterior chamber of the eye of the same animal. Longterm grafts showed small taste buds located in epitelial cavities which were probably the remnants of trench walls. These grafts have been reinnervated by local nerves. Excretory ducts and serous acini of Von Ebner's glands were also present. The latter showed secretory granules in their cells. The specificity of the nerve fibers and of the epithelium in relation to the induction of taste bud differentiation are discussed."}
{"id": "PMID:464322", "title": "Development of the intrarenal vascular system of the puppy kidney.", "content": "This study investigates the development of the vascular system of the puppy kidney (1-21 days after birth) after preparing casts of the renal vessels. At two days, the intrarenal vascular system distal to the afferent arteriole is strikingly different than that of the adult. The glomeruli of the outer cortex consist of a single dilated vessel while those of the mid and inner cortex posses an increasingly larger number of capillary loops. The efferent arterioles vary greatly in appearance from outer to inner cortex. Those in the nephrogenic zone are characteristically short and narrow and join a larger venous vessel termined a sinusoidal capillary. An efferent system somewhat similar to that of the adult is seen in the mid and inner cortex. One of the most obvious differences noted between the puppy and adult kidney is the relative lack of peritubular capillary networks throughout the cortex of the puppy kidney. The puppy possesses large, irregular vessels termed sinusoidal capillaries. The most rudimentary sinusoids are found in the outer cortex with more mature vessels in the inner cortex. The vascular arrangement of the efferent arteriole and sinusoidal capillary appears as a post-glomerular shunt. Functionally, the shunt would direct blood flow away from the proximal tubule and thus could result in a low extraction ratio and Tm for secreted solutes.", "contents": "Development of the intrarenal vascular system of the puppy kidney. This study investigates the development of the vascular system of the puppy kidney (1-21 days after birth) after preparing casts of the renal vessels. At two days, the intrarenal vascular system distal to the afferent arteriole is strikingly different than that of the adult. The glomeruli of the outer cortex consist of a single dilated vessel while those of the mid and inner cortex posses an increasingly larger number of capillary loops. The efferent arterioles vary greatly in appearance from outer to inner cortex. Those in the nephrogenic zone are characteristically short and narrow and join a larger venous vessel termined a sinusoidal capillary. An efferent system somewhat similar to that of the adult is seen in the mid and inner cortex. One of the most obvious differences noted between the puppy and adult kidney is the relative lack of peritubular capillary networks throughout the cortex of the puppy kidney. The puppy possesses large, irregular vessels termed sinusoidal capillaries. The most rudimentary sinusoids are found in the outer cortex with more mature vessels in the inner cortex. The vascular arrangement of the efferent arteriole and sinusoidal capillary appears as a post-glomerular shunt. Functionally, the shunt would direct blood flow away from the proximal tubule and thus could result in a low extraction ratio and Tm for secreted solutes."}
{"id": "PMID:464325", "title": "Impregnation of soft biological specimens with thermosetting resins and elastomers.", "content": "A new method for impregnation of biological specimens with thermosetting resins and elastomers is described. The method has the advantage that the original relief of the surface is retained. The impregnation is carried out by utilizing the difference between the high vapor tension of the intermedium (e.g., methylene chloride) and the low vapor tension of the solution to be polymerized. After impregnation, the specimen is subject to polymerization conditions without surrounding embedding material. The optical and mechanical properties can be selected by proper choice from various kinds of resins and different procedures, for example, by complete or incomplete impregnation. Acrylic resins, polyester resins, epoxy resins, polyurethanes and silicone rubber have been found suitable for the method. Excellent results have been obtained using transparent silicone rubber since after treatment the specimens are still flexible and resilient, and have retained their natural appearance.", "contents": "Impregnation of soft biological specimens with thermosetting resins and elastomers. A new method for impregnation of biological specimens with thermosetting resins and elastomers is described. The method has the advantage that the original relief of the surface is retained. The impregnation is carried out by utilizing the difference between the high vapor tension of the intermedium (e.g., methylene chloride) and the low vapor tension of the solution to be polymerized. After impregnation, the specimen is subject to polymerization conditions without surrounding embedding material. The optical and mechanical properties can be selected by proper choice from various kinds of resins and different procedures, for example, by complete or incomplete impregnation. Acrylic resins, polyester resins, epoxy resins, polyurethanes and silicone rubber have been found suitable for the method. Excellent results have been obtained using transparent silicone rubber since after treatment the specimens are still flexible and resilient, and have retained their natural appearance."}
{"id": "PMID:464326", "title": "Ultrastructure of the fetal rat anterior pituitary gland as term and during prolonged gestation.", "content": "The ultrastructure of the fetal rat anterior pituitary gland is described at term (day 22) and during experimentally prolonged gestation (days 23, 24, 25). The latter was achieved by daily subcutaneous injections of five mg progesterone to gravid females from the twentieth through the twenty-fourth day. Using morphological criteria for classifying pituitary cells (Moriarty, '73), six different types were observed: thyrotrophs, gonadotrophs, corticotrophs, mammotrophs, somatotrophs and non-granulated cells. During prolonged gestation (days 24 and 25 only), cells designated as corticotrophs revealed changes suggesting increased activity, i.e.,proliferation and dilation of the endoplasmic reticulum, dilated Golgi membranes and a redction of secretory granules. In addition, meconium staining , which is indicative of fetal distress, was also observed on days 24 and 25. The ultrastructural changes noted suggest an increased in corticotroph activity due to fetal hypoglycemia and hypoxia which are known to occur as a result of placental insufficiency during prolonged gestation (Roux et al., '64; Vorherr, '75; Thliveris, '76.", "contents": "Ultrastructure of the fetal rat anterior pituitary gland as term and during prolonged gestation. The ultrastructure of the fetal rat anterior pituitary gland is described at term (day 22) and during experimentally prolonged gestation (days 23, 24, 25). The latter was achieved by daily subcutaneous injections of five mg progesterone to gravid females from the twentieth through the twenty-fourth day. Using morphological criteria for classifying pituitary cells (Moriarty, '73), six different types were observed: thyrotrophs, gonadotrophs, corticotrophs, mammotrophs, somatotrophs and non-granulated cells. During prolonged gestation (days 24 and 25 only), cells designated as corticotrophs revealed changes suggesting increased activity, i.e.,proliferation and dilation of the endoplasmic reticulum, dilated Golgi membranes and a redction of secretory granules. In addition, meconium staining , which is indicative of fetal distress, was also observed on days 24 and 25. The ultrastructural changes noted suggest an increased in corticotroph activity due to fetal hypoglycemia and hypoxia which are known to occur as a result of placental insufficiency during prolonged gestation (Roux et al., '64; Vorherr, '75; Thliveris, '76."}
{"id": "PMID:464327", "title": "The distribution of alkaline phosphatase activity in normal and cross-species regenerated rat and mouse taste buds.", "content": "Alkaline phosphatase (ALK Pase) activity can be detected histochemically in the taste buds of rats but not mice. Since taste buds develop, regenerate and are maintained under the influence(s) of the sensory nerve it was decided to study cross-species regenerated buds of these two animals to determine whether the nerve also regulated ALK Pase development in taste cells. Grafts of rats sensory ganglion and mouse tongue or mouse ganglion and rat tongue were combined in the anterior chamber of the eyes of immunologically-deficient nude mice and the cross-species buds that developed at 35 days were examined histochemically for ALK Pase. The results revealed that the rat nerve did not cause ALK Pase to appear in any buds found in mouse tongue grafts and that mouse nerve could support buds containing ALK Pase in rat tongue tissue. Because the cross-species regenerated buds were histochemically characteristic of those normally found in rat or mouse tongue, there is no evidence that the foreign nerve altered gene expression for ALK Pase in the target organ, and the action of the nerve on gustatory epithelium appears to be that of activation and maintenance.", "contents": "The distribution of alkaline phosphatase activity in normal and cross-species regenerated rat and mouse taste buds. Alkaline phosphatase (ALK Pase) activity can be detected histochemically in the taste buds of rats but not mice. Since taste buds develop, regenerate and are maintained under the influence(s) of the sensory nerve it was decided to study cross-species regenerated buds of these two animals to determine whether the nerve also regulated ALK Pase development in taste cells. Grafts of rats sensory ganglion and mouse tongue or mouse ganglion and rat tongue were combined in the anterior chamber of the eyes of immunologically-deficient nude mice and the cross-species buds that developed at 35 days were examined histochemically for ALK Pase. The results revealed that the rat nerve did not cause ALK Pase to appear in any buds found in mouse tongue grafts and that mouse nerve could support buds containing ALK Pase in rat tongue tissue. Because the cross-species regenerated buds were histochemically characteristic of those normally found in rat or mouse tongue, there is no evidence that the foreign nerve altered gene expression for ALK Pase in the target organ, and the action of the nerve on gustatory epithelium appears to be that of activation and maintenance."}
{"id": "PMID:464328", "title": "Muscle proteins and the changes in shape of avian oxynticopeptic cells in relation to secretion.", "content": "A combined ultrastructural and biochemical study of the avian oxynticopeptic cell was performed. Scanning electron microscopy demonstrates that this cell undergoes great changes in the shape of its apical pole in relation to secretory activity. These changes are confirmed by transmission electron microscopy and by freeze-fracture images. The biochemical finding of actin- and myosin-like proteins in high-speed supernatants of homogenates of these cells as well as the ultrastructural and cytochemical localization of actin-like filaments in their apical poles suggest a possible participation of these proteins in the above-mentioned changes. Thus, the study of cytoplasmic matrix elements and of their organization may be highly relevant in the search for a correlation between structure and function in these cells.", "contents": "Muscle proteins and the changes in shape of avian oxynticopeptic cells in relation to secretion. A combined ultrastructural and biochemical study of the avian oxynticopeptic cell was performed. Scanning electron microscopy demonstrates that this cell undergoes great changes in the shape of its apical pole in relation to secretory activity. These changes are confirmed by transmission electron microscopy and by freeze-fracture images. The biochemical finding of actin- and myosin-like proteins in high-speed supernatants of homogenates of these cells as well as the ultrastructural and cytochemical localization of actin-like filaments in their apical poles suggest a possible participation of these proteins in the above-mentioned changes. Thus, the study of cytoplasmic matrix elements and of their organization may be highly relevant in the search for a correlation between structure and function in these cells."}
{"id": "PMID:464329", "title": "[Clinical, endocrinological, histological and chromosomal investigations on Klinefelter's syndrome].", "content": "In a clinical study 101 patients with Klinefelter's syndrome (Kl. sy.) are evaluated. Clinical, endocrinological and histometrical aspects were of main interest. The results were compared with a group of patients with azoospermia by obstruction. The statistical evaluation showed significant differences concerning body-height, volume of ejaculate, size of testis, and the concentration of FSH and LH in serum. Histometrical investigations showed significant differences for the diameter of seminiferous tubules and their wall-thickness. The various degrees of Leydig-cell-increase at Kl. Sy. are described as--increased--excessively increased--and adenomalike. Sex-Chromatin was found positive in 62 of 64 cases of Klinefelter's syndrome, verified by chromosome analysis. The number of Barr-bodies ranged from 1-45 (200 cells evaluated) x mean was 9,6. The analysis of chromosomes in 64 cases showed a karyotype of 47,XXY in 59 cases, a 46, XY/47,XXY mosaic in 4 cases and 46,XX/46,XY/47,XXY mosaic in 1 case. The discussion pointed out, that Kl. sy. has no typical, but a variety of symptoms. The position of the evaluation of sex-chromatin for diagnosis and the possibilities of errors are discussed. The problem of fertility in Kl. sy. is mentioned, as we found two cases with complete spermatogenesis, yet with azoospermia, among our patients. The pathogenesis of the characteristical histological changes in testis with Kl. sy. remains open. The mechanism which may lead to the striking increase of Leydig-cells is discussed.", "contents": "[Clinical, endocrinological, histological and chromosomal investigations on Klinefelter's syndrome]. In a clinical study 101 patients with Klinefelter's syndrome (Kl. sy.) are evaluated. Clinical, endocrinological and histometrical aspects were of main interest. The results were compared with a group of patients with azoospermia by obstruction. The statistical evaluation showed significant differences concerning body-height, volume of ejaculate, size of testis, and the concentration of FSH and LH in serum. Histometrical investigations showed significant differences for the diameter of seminiferous tubules and their wall-thickness. The various degrees of Leydig-cell-increase at Kl. Sy. are described as--increased--excessively increased--and adenomalike. Sex-Chromatin was found positive in 62 of 64 cases of Klinefelter's syndrome, verified by chromosome analysis. The number of Barr-bodies ranged from 1-45 (200 cells evaluated) x mean was 9,6. The analysis of chromosomes in 64 cases showed a karyotype of 47,XXY in 59 cases, a 46, XY/47,XXY mosaic in 4 cases and 46,XX/46,XY/47,XXY mosaic in 1 case. The discussion pointed out, that Kl. sy. has no typical, but a variety of symptoms. The position of the evaluation of sex-chromatin for diagnosis and the possibilities of errors are discussed. The problem of fertility in Kl. sy. is mentioned, as we found two cases with complete spermatogenesis, yet with azoospermia, among our patients. The pathogenesis of the characteristical histological changes in testis with Kl. sy. remains open. The mechanism which may lead to the striking increase of Leydig-cells is discussed."}
{"id": "PMID:464330", "title": "Leydig cell differentiation induced by stimulation with HCG and HMG in two patients affected with hypogonadotropic hypogonadism.", "content": "In order to elucidate the ultrastructural characteristics of the precursor cell of Leydig cells and its subsequent differentiation, testicular biopsies from two patients with hypogonadotropic hypogonadism, obtained prior to and after treatment with HCG and HMG, were studied in comparison with those of prepubertal, pubertal and postpubertal testes. Prior to HCG and HMG stimulation, the testicular interstitium of both patients showed the following spindle-cell-types: Fibroblasts, myofibroblasts and a cell-type similar to the myofibroblast but differing from it by the arrangement of microfilaments and the development of the smooth endoplasmic reticulum as well as lipid droplets. It may be considered the precursor of Leydig cells. In the following stages of differentiation observed in the biopsies after treatment, there is a progressive involution of microfilaments and rough endoplasmic reticulum, a transient period of hyperplasia of the Golgi complex, and the definitive development of cell components involved in steroid biosynthesis, such as the smooth endoplasmic reticulum, polymorphic mitochondria with tubular cristae, lipid droplets and diverse lysosomal bodies. From early stages of differentiation, gap junctions occurred between the cells. Numerous axon profiles were also present among the cells. Only in a small percentage of cells did vesicle-bearing axons closely appose the Leydig cell membrane.", "contents": "Leydig cell differentiation induced by stimulation with HCG and HMG in two patients affected with hypogonadotropic hypogonadism. In order to elucidate the ultrastructural characteristics of the precursor cell of Leydig cells and its subsequent differentiation, testicular biopsies from two patients with hypogonadotropic hypogonadism, obtained prior to and after treatment with HCG and HMG, were studied in comparison with those of prepubertal, pubertal and postpubertal testes. Prior to HCG and HMG stimulation, the testicular interstitium of both patients showed the following spindle-cell-types: Fibroblasts, myofibroblasts and a cell-type similar to the myofibroblast but differing from it by the arrangement of microfilaments and the development of the smooth endoplasmic reticulum as well as lipid droplets. It may be considered the precursor of Leydig cells. In the following stages of differentiation observed in the biopsies after treatment, there is a progressive involution of microfilaments and rough endoplasmic reticulum, a transient period of hyperplasia of the Golgi complex, and the definitive development of cell components involved in steroid biosynthesis, such as the smooth endoplasmic reticulum, polymorphic mitochondria with tubular cristae, lipid droplets and diverse lysosomal bodies. From early stages of differentiation, gap junctions occurred between the cells. Numerous axon profiles were also present among the cells. Only in a small percentage of cells did vesicle-bearing axons closely appose the Leydig cell membrane."}
{"id": "PMID:464332", "title": "Silent infection in male accessory genital organs and male infertility.", "content": "A silent infection of the male accessory genital organs was found in the semen of 78 patients (22,3%) of 350 infertile men. All the sperm characteristics (except agglutination) were significantly deteriorated in the infection group as compared to the sperm of 36 normal men. The volume was significantly larger than normal. In this group there was significant leukocytosis and, as a sign of prostatic hypofunction, reduced seminal acid phosphatase and citric acid activity. Chemotherapy for a period of two to four weeks improved leukocytosis and sperm transiently. Only acid phosphatase was normal three months after the treatment. Suggested reasons for the infertility of the silent infection group are: 1. hypofunction of the male genital organs, caused by a primary, strong infection suffered earlier. 2. The chronic, nonclinical infection of the male accessory genital organs, mainly as a complication of the primary infection. Chemotherapy for a period of four weeks seems to be too short to correct the silent infection as well as the infertility.", "contents": "Silent infection in male accessory genital organs and male infertility. A silent infection of the male accessory genital organs was found in the semen of 78 patients (22,3%) of 350 infertile men. All the sperm characteristics (except agglutination) were significantly deteriorated in the infection group as compared to the sperm of 36 normal men. The volume was significantly larger than normal. In this group there was significant leukocytosis and, as a sign of prostatic hypofunction, reduced seminal acid phosphatase and citric acid activity. Chemotherapy for a period of two to four weeks improved leukocytosis and sperm transiently. Only acid phosphatase was normal three months after the treatment. Suggested reasons for the infertility of the silent infection group are: 1. hypofunction of the male genital organs, caused by a primary, strong infection suffered earlier. 2. The chronic, nonclinical infection of the male accessory genital organs, mainly as a complication of the primary infection. Chemotherapy for a period of four weeks seems to be too short to correct the silent infection as well as the infertility."}
{"id": "PMID:464340", "title": "Hemodynamic effects of phentolamine in mitral stenosis and congestive cardiomyopahty.", "content": "Nine patients with alcoholic cardiomyopathy and 8 patients with mitral stenosis were studied by right and left heart catheterization. Hemodynamic observations were made during control period and during the infusion of phentolamine at a rate of 0.3 mg per minute. The patients with the cardiomyopathy responded to phentolamine with a significant decline in the left ventricular filling pressure, pulmonary artery mean pressure, peripheral resistance, and arteriovenous oxygen difference. There was a significant increase in the cardiac index. The patients with mitral stenosis did not show any hemodynamic improvement after phentolamine infusion.", "contents": "Hemodynamic effects of phentolamine in mitral stenosis and congestive cardiomyopahty. Nine patients with alcoholic cardiomyopathy and 8 patients with mitral stenosis were studied by right and left heart catheterization. Hemodynamic observations were made during control period and during the infusion of phentolamine at a rate of 0.3 mg per minute. The patients with the cardiomyopathy responded to phentolamine with a significant decline in the left ventricular filling pressure, pulmonary artery mean pressure, peripheral resistance, and arteriovenous oxygen difference. There was a significant increase in the cardiac index. The patients with mitral stenosis did not show any hemodynamic improvement after phentolamine infusion."}
{"id": "PMID:464343", "title": "Effect of digitalis on diminished baroreceptor sensitivity in diabetics.", "content": "The baroreceptor sensitivity was estimated in 50 normal controls (Group A) and in 50 diabetics of comparable age (Group B). The technique used was infusion of angiotensin (0.5 microgram/min) and measurement of the bradycardic response resulting from the increase of blood pressure. The slope was used as an index of baroreceptor sensitivity. Diabetics had significantly lower baroreceptor sensitivity and a higher resting heart rate. Sensitivity decreased with age in both groups. The reproducibility of the method was excellent. Deslanoside-C (0.8 mg) significantly increased the baroreceptor sensitivity in 11 normal controls and 9 diabetics. Very low sensitivity was found in 26 diabetics who had no evidence of orthostatic hypotension, neuropathy, or retinopathy. However all 17 patients with the above findings had very low sensitivity.", "contents": "Effect of digitalis on diminished baroreceptor sensitivity in diabetics. The baroreceptor sensitivity was estimated in 50 normal controls (Group A) and in 50 diabetics of comparable age (Group B). The technique used was infusion of angiotensin (0.5 microgram/min) and measurement of the bradycardic response resulting from the increase of blood pressure. The slope was used as an index of baroreceptor sensitivity. Diabetics had significantly lower baroreceptor sensitivity and a higher resting heart rate. Sensitivity decreased with age in both groups. The reproducibility of the method was excellent. Deslanoside-C (0.8 mg) significantly increased the baroreceptor sensitivity in 11 normal controls and 9 diabetics. Very low sensitivity was found in 26 diabetics who had no evidence of orthostatic hypotension, neuropathy, or retinopathy. However all 17 patients with the above findings had very low sensitivity."}
{"id": "PMID:464344", "title": "Medical management of hypertension.", "content": "In a paper like this, with such diverse information, it is impossible to summarize the content completely. Suffice it to say that a stepwise approach to the management of essential hypertension has been presented, beginning with a diuretic agent as the cornerstone of management for most patients. Recommendations for the addition of a second, third, or even a fourth agent have been offered based on a discussion of the site of action of various therapeutic agents. The common side effects and toxicity of various drugs have also been discussed. It cannot be overemphasized that the physician's knowledge of his patient and a thorough understanding of drug actions, interactions, and reactions are mandatory if highly effective, individualized therapeutic programs are to be implemented. Likewise, educating the patient about his disease and eliciting his maximal cooperation are keys to therapeutic success.", "contents": "Medical management of hypertension. In a paper like this, with such diverse information, it is impossible to summarize the content completely. Suffice it to say that a stepwise approach to the management of essential hypertension has been presented, beginning with a diuretic agent as the cornerstone of management for most patients. Recommendations for the addition of a second, third, or even a fourth agent have been offered based on a discussion of the site of action of various therapeutic agents. The common side effects and toxicity of various drugs have also been discussed. It cannot be overemphasized that the physician's knowledge of his patient and a thorough understanding of drug actions, interactions, and reactions are mandatory if highly effective, individualized therapeutic programs are to be implemented. Likewise, educating the patient about his disease and eliciting his maximal cooperation are keys to therapeutic success."}
{"id": "PMID:464345", "title": "Hypertension detection and compliance: permanent site hypertensive evaluation--a new method of increasing patient compliance.", "content": "A new method--permanent site hypertensive evaluation with patient revisits--is suggested as a unique way to increase patient compliance. This method reduced mild hypertension for 50% and increased normotension. The easy availability of permanent sites may induce the patient to return regularly, or the patient may be testing the medication or his physician. But whatever the means, normotension is the aim of compliance. Thanks to Mark Cohen and his pharmacy staff, Dot, Ruth, Jim, and George for their many long hours of detection work; to my nurse monitor, Pauline Platt; and to my secretary, Gene Koswoski. This study was done in cooperation with the Montgomery County Branch of the Southeastern Pennsylvania Chapter of the American Heart Association, Philadelphia, Pennsylvania.", "contents": "Hypertension detection and compliance: permanent site hypertensive evaluation--a new method of increasing patient compliance. A new method--permanent site hypertensive evaluation with patient revisits--is suggested as a unique way to increase patient compliance. This method reduced mild hypertension for 50% and increased normotension. The easy availability of permanent sites may induce the patient to return regularly, or the patient may be testing the medication or his physician. But whatever the means, normotension is the aim of compliance. Thanks to Mark Cohen and his pharmacy staff, Dot, Ruth, Jim, and George for their many long hours of detection work; to my nurse monitor, Pauline Platt; and to my secretary, Gene Koswoski. This study was done in cooperation with the Montgomery County Branch of the Southeastern Pennsylvania Chapter of the American Heart Association, Philadelphia, Pennsylvania."}
{"id": "PMID:464349", "title": "Reproducibility of the response to diluent challenge in adult asthma.", "content": "The authors have demonstrated that patients with bronchial asthma may react to diluent provocative challenge. The pattern of airway response to diluent was found to be reproducible. The data herewith presented suggest that the baseline airway obstruction in adult asthma is highly variable but the degree of baseline obstruction is not related to the response to diluent challenge.", "contents": "Reproducibility of the response to diluent challenge in adult asthma. The authors have demonstrated that patients with bronchial asthma may react to diluent provocative challenge. The pattern of airway response to diluent was found to be reproducible. The data herewith presented suggest that the baseline airway obstruction in adult asthma is highly variable but the degree of baseline obstruction is not related to the response to diluent challenge."}
{"id": "PMID:464350", "title": "Factors of importance in determining the prevalence of indoor molds.", "content": "The concentration of molds isolated in 68 homes of allergic patients in southern California using the Andersen volumetric sampler varied from a minimum of 36 to a maximum of 5,984 isolate/M3 air sampled. The most frequently isolated included Cladosporium, Penicillium species. Alternaria, Sterile (Non-sporulating) Mycelium, Epicoccum, Aspergillus species, Aureobasidium and Dreschlera. Statistically significant higher mold isolates were associated with high shade and high levels of organic debris near the home and poor landscaping and landscape maintenance. Low concentrations of mold isolates were associated with the presence of a central electrostatic filtration system and good compliance with dust controls. The viable mold spore levels were lower in homes where the electrostatic filtration unit was operated continuously rather than intermittently. No statistically significant correlations could be made between indoor mold isolates and any of the following: number and age of the occupants, age and size of home, month of survey or the presence of indoor plants.", "contents": "Factors of importance in determining the prevalence of indoor molds. The concentration of molds isolated in 68 homes of allergic patients in southern California using the Andersen volumetric sampler varied from a minimum of 36 to a maximum of 5,984 isolate/M3 air sampled. The most frequently isolated included Cladosporium, Penicillium species. Alternaria, Sterile (Non-sporulating) Mycelium, Epicoccum, Aspergillus species, Aureobasidium and Dreschlera. Statistically significant higher mold isolates were associated with high shade and high levels of organic debris near the home and poor landscaping and landscape maintenance. Low concentrations of mold isolates were associated with the presence of a central electrostatic filtration system and good compliance with dust controls. The viable mold spore levels were lower in homes where the electrostatic filtration unit was operated continuously rather than intermittently. No statistically significant correlations could be made between indoor mold isolates and any of the following: number and age of the occupants, age and size of home, month of survey or the presence of indoor plants."}
{"id": "PMID:464351", "title": "Non-compliance in asthmatic children: a study of theophylline levels in a pediatric emergency room population.", "content": "Sub-therapeutic theophylline levels due to patient (parent) non-compliance is a significant cause of outpatient treatment failure in childhood asthma. In a study of 50 known asthmatic children with acute asthmatic episodes, 98% had sub-therapeutic theophylline levels less than 10 mcg/ml with 75.5% due to inadequate patient (parent) compliance.", "contents": "Non-compliance in asthmatic children: a study of theophylline levels in a pediatric emergency room population. Sub-therapeutic theophylline levels due to patient (parent) non-compliance is a significant cause of outpatient treatment failure in childhood asthma. In a study of 50 known asthmatic children with acute asthmatic episodes, 98% had sub-therapeutic theophylline levels less than 10 mcg/ml with 75.5% due to inadequate patient (parent) compliance."}
{"id": "PMID:464352", "title": "Immunization of chickens and turkeys against avian influenza with monovalent and polyvalent oil emulsion vaccines.", "content": "Chickens and turkeys vaccinated with inactivated virus oil-emulsion vaccines containing different concentrations of either 1 (monovalent) or 4 (polyvalent) strains of avian influenza virus (AIV) were challenged-exposed with virulent AIV A/chicken/Scotland/59 or A/turkey/Ontario/7732/66. Four of 6 vaccines protected completely against postexposure mortality. Vaccine valency did not alter the serologic and challenge-exposure responses of chickens vaccinated with AIV A/turkey/Wisconsin/68, which was the virus component common to both monovalent and polyvalent vaccines. The magnitude of the serologic responses and protection against challenge-exposure were dependent on the concentration of virus in the vaccines. These data indicate that control of virulent AIV in chickens and turkeys by vaccination with inactivated vaccines may be feasible.", "contents": "Immunization of chickens and turkeys against avian influenza with monovalent and polyvalent oil emulsion vaccines. Chickens and turkeys vaccinated with inactivated virus oil-emulsion vaccines containing different concentrations of either 1 (monovalent) or 4 (polyvalent) strains of avian influenza virus (AIV) were challenged-exposed with virulent AIV A/chicken/Scotland/59 or A/turkey/Ontario/7732/66. Four of 6 vaccines protected completely against postexposure mortality. Vaccine valency did not alter the serologic and challenge-exposure responses of chickens vaccinated with AIV A/turkey/Wisconsin/68, which was the virus component common to both monovalent and polyvalent vaccines. The magnitude of the serologic responses and protection against challenge-exposure were dependent on the concentration of virus in the vaccines. These data indicate that control of virulent AIV in chickens and turkeys by vaccination with inactivated vaccines may be feasible."}
{"id": "PMID:464353", "title": "A proposed mechanism(s) of transitory ischemic injury to myocardium.", "content": "The main objective of this study was to produce primary acute ischemic injury to myocardium in a live animal. In vitro, guinea pig platelets were sensitive to perturbation and aggregation by a suspension of ultrafine fibrillary collagen material isolated from the aorta of an aged burro (Equus asinus). The platelets responded to the stimulatory action of this material down to 100 to 200 ng (dry weight) added to 0.45 ml of platelet-rich plasma, as determined by aggregometric technique. Aortic fibrillary collagen material injected IV into guinea pigs (350 to 1900 microgram protein/kg of weight) produced a transitory disappearance of virtually all circulating platelets within 5 minutes. In animals in which blood samples were taken 2.5 hours after injection, 50 to 75% of the average base-line platelets in the circulation of controls returned to the circulation. In other experiments, 3 anesthetized animals were injected by jugular vein with an amount of active fibrillary collagen material (300 microgram as protein/kg of animal weight) estimated to produce reversible platelet aggregation in vivo. Two control animals were injected with the same dose of the material that had been inactivated (15 minutes at 100 C) to abolish platelet aggregation. Treated and control animals were maintained under general anesthesia for 2.5 hours. Intraventricular pressures and electrocardiographs (ECG) were monitored continuously for the first 30 minutes. The injection of the active fibrillary collagen material caused a large ventricular pressure elevation (170/10, 180/10, and 150/10 mm of Hg) in approximately 40 s. Preinfusion ventricular pressures in the 3 animals were 65/0, 85/5, and 88/0 mm of Hg, respectively. Within 60 s, there was a reduction in the absolute platelet number in the peripheral circulation. The elevation of ventricular pressure persisted for approximately 5 minutes and was followed within 30 minutes by a set of ECG events suggestive of acute myocardial ischemic injury, which included premature ventricular contractions, transient S-T segment depression concurrent with ventricular hypertension, and S-T segment elevation with reversed tall upright T-waves in association with a decrease to the preinfusion ventricular base line. Other ECG changes included prolongation of the P-R segment, missed ventricular contractions, and arrhythmia. The ECG changes seemed to be subsequent to platelet microthrombus formation in the pulmonary arterial microcirculation. By 2.5 hours after the treatment, platelets \"rebounded\" into the circulation in 2 surviving guinea pigs, and left ventricular pressures and ECG profiles returned to the preinfusion base lines. Guinea pigs IV infused with similar amounts of inactivated (15 minutes at 100 C) fibrillary collagen material did not show changes.", "contents": "A proposed mechanism(s) of transitory ischemic injury to myocardium. The main objective of this study was to produce primary acute ischemic injury to myocardium in a live animal. In vitro, guinea pig platelets were sensitive to perturbation and aggregation by a suspension of ultrafine fibrillary collagen material isolated from the aorta of an aged burro (Equus asinus). The platelets responded to the stimulatory action of this material down to 100 to 200 ng (dry weight) added to 0.45 ml of platelet-rich plasma, as determined by aggregometric technique. Aortic fibrillary collagen material injected IV into guinea pigs (350 to 1900 microgram protein/kg of weight) produced a transitory disappearance of virtually all circulating platelets within 5 minutes. In animals in which blood samples were taken 2.5 hours after injection, 50 to 75% of the average base-line platelets in the circulation of controls returned to the circulation. In other experiments, 3 anesthetized animals were injected by jugular vein with an amount of active fibrillary collagen material (300 microgram as protein/kg of animal weight) estimated to produce reversible platelet aggregation in vivo. Two control animals were injected with the same dose of the material that had been inactivated (15 minutes at 100 C) to abolish platelet aggregation. Treated and control animals were maintained under general anesthesia for 2.5 hours. Intraventricular pressures and electrocardiographs (ECG) were monitored continuously for the first 30 minutes. The injection of the active fibrillary collagen material caused a large ventricular pressure elevation (170/10, 180/10, and 150/10 mm of Hg) in approximately 40 s. Preinfusion ventricular pressures in the 3 animals were 65/0, 85/5, and 88/0 mm of Hg, respectively. Within 60 s, there was a reduction in the absolute platelet number in the peripheral circulation. The elevation of ventricular pressure persisted for approximately 5 minutes and was followed within 30 minutes by a set of ECG events suggestive of acute myocardial ischemic injury, which included premature ventricular contractions, transient S-T segment depression concurrent with ventricular hypertension, and S-T segment elevation with reversed tall upright T-waves in association with a decrease to the preinfusion ventricular base line. Other ECG changes included prolongation of the P-R segment, missed ventricular contractions, and arrhythmia. The ECG changes seemed to be subsequent to platelet microthrombus formation in the pulmonary arterial microcirculation. By 2.5 hours after the treatment, platelets \"rebounded\" into the circulation in 2 surviving guinea pigs, and left ventricular pressures and ECG profiles returned to the preinfusion base lines. Guinea pigs IV infused with similar amounts of inactivated (15 minutes at 100 C) fibrillary collagen material did not show changes."}
{"id": "PMID:464354", "title": "Body water content and turnover in cats fed dry and canned rations.", "content": "Body water content and total body water turnover in cats fed commercially available dry food and then given canned rations were determined with tritiated water. Cats during the feeding of either ration did not differ in body water content or turnover. Cats during the feeding of the dry ration derived a greater fraction of their total water turnover from drinking water, and these cats drank more water per gram of dry matter intake than when fed the canned ration. On the basis of total water intake, however, those given the canned ration had significantly greater water intake per gram of dry matter; also, their total water turnover per gram of dry matter was greater. Compared with other animals, cats have a similar ratio of body water to body solids, but the rate of water turnover per unit of body weight is slower.", "contents": "Body water content and turnover in cats fed dry and canned rations. Body water content and total body water turnover in cats fed commercially available dry food and then given canned rations were determined with tritiated water. Cats during the feeding of either ration did not differ in body water content or turnover. Cats during the feeding of the dry ration derived a greater fraction of their total water turnover from drinking water, and these cats drank more water per gram of dry matter intake than when fed the canned ration. On the basis of total water intake, however, those given the canned ration had significantly greater water intake per gram of dry matter; also, their total water turnover per gram of dry matter was greater. Compared with other animals, cats have a similar ratio of body water to body solids, but the rate of water turnover per unit of body weight is slower."}
{"id": "PMID:464355", "title": "Elaboration of chemotactic substances by alveolar cells: possible mechanisms for the initial neutrophilic response in feline caliciviral pneumonia.", "content": "In vitro experiments established that the interaction of feline calicivirus (FCV) with alveolar macrophages and pneumocytes results in the generation of chemotactic factors that produce directed migration of neutrophils in Boyden chambers. Factors were produced independent of immune mechanisms and of discernible serum factors. Lysates of noninfected alveolar macrophages and pneumocytes did not possess chemotactic activity, indicating that chemotactic factors were not preformed in these cells. Noninfected alveolar macrophages also elaborated neutrophil chemotactic factors in culture; however, activity was greater when macrophages were infected with FCV. The results of this study suggested that the neutrophilic response in the peripheral portion of the lung of cats exposed to aerosols of FCV was the result of the elaboration of complete chemotactic factors from FCV-infected alveolar cells.", "contents": "Elaboration of chemotactic substances by alveolar cells: possible mechanisms for the initial neutrophilic response in feline caliciviral pneumonia. In vitro experiments established that the interaction of feline calicivirus (FCV) with alveolar macrophages and pneumocytes results in the generation of chemotactic factors that produce directed migration of neutrophils in Boyden chambers. Factors were produced independent of immune mechanisms and of discernible serum factors. Lysates of noninfected alveolar macrophages and pneumocytes did not possess chemotactic activity, indicating that chemotactic factors were not preformed in these cells. Noninfected alveolar macrophages also elaborated neutrophil chemotactic factors in culture; however, activity was greater when macrophages were infected with FCV. The results of this study suggested that the neutrophilic response in the peripheral portion of the lung of cats exposed to aerosols of FCV was the result of the elaboration of complete chemotactic factors from FCV-infected alveolar cells."}
{"id": "PMID:464356", "title": "Comparison of the cardiopulmonary effects of etomidate and thiamylal in dogs.", "content": "The cardiopulmonary effects of etomidate, a nonbarbiturate, short-acting, IV anesthetic, were compared and contrasted with those of thiamylal sodium in chronically instrumented conscious dogs. Etomidate, when administered IV at dosages of 1.5 and 3.0 mg/kg of body weight, produced anesthesia lasting from 8 +/- 5 and 21 +/- 9 minutes, respectively. Heart rate, aortic blood pressure, left ventricular peak pressure, left ventricular end diastolic pressure, left ventricular contractile force, and myocardial oxygen consumption were unchanged after administration of either dose of etomidate; however, the dosage of 1.5 mg/kg produced significant (P less than 0.05) increases in respiratory rate and decreases in tidal volume. The minute volume remained unchanged from base-line values. Significant (P less than 0.05) decreases in tidal volume, arterial pH, and partial pressure of oxygen were produced, and minute volume remained unchanged when 3.0 mg of etomidate/kg of body weight was administered. Thiamylal sodium (8.0 mg/kg of body weight; given IV) produced anesthesia lasting for 14 +/- 5 minutes. Significant increases (P less than 0.05) in heart rate, arterial blood pressure, left ventricular peak pressure, and myocardial oxygen consumption were observed after IV administration. Left ventricular contractility was significantly (P less than 0.05) decreased. Respiratory rate was not significantly (P less than 0.05) affected by thiamylal although tidal volume and minute volume were decreased. These respiratory alterations resulted in significant (P less than 0.05) increases in the arterial partial pressure of carbon dioxide and decreases in pH and the partial pressure of oxygen. On the basis of cardiopulmonary function, etomidate offered rapid, safe, short duration anesthesia superior to that of thiamylal sodium.", "contents": "Comparison of the cardiopulmonary effects of etomidate and thiamylal in dogs. The cardiopulmonary effects of etomidate, a nonbarbiturate, short-acting, IV anesthetic, were compared and contrasted with those of thiamylal sodium in chronically instrumented conscious dogs. Etomidate, when administered IV at dosages of 1.5 and 3.0 mg/kg of body weight, produced anesthesia lasting from 8 +/- 5 and 21 +/- 9 minutes, respectively. Heart rate, aortic blood pressure, left ventricular peak pressure, left ventricular end diastolic pressure, left ventricular contractile force, and myocardial oxygen consumption were unchanged after administration of either dose of etomidate; however, the dosage of 1.5 mg/kg produced significant (P less than 0.05) increases in respiratory rate and decreases in tidal volume. The minute volume remained unchanged from base-line values. Significant (P less than 0.05) decreases in tidal volume, arterial pH, and partial pressure of oxygen were produced, and minute volume remained unchanged when 3.0 mg of etomidate/kg of body weight was administered. Thiamylal sodium (8.0 mg/kg of body weight; given IV) produced anesthesia lasting for 14 +/- 5 minutes. Significant increases (P less than 0.05) in heart rate, arterial blood pressure, left ventricular peak pressure, and myocardial oxygen consumption were observed after IV administration. Left ventricular contractility was significantly (P less than 0.05) decreased. Respiratory rate was not significantly (P less than 0.05) affected by thiamylal although tidal volume and minute volume were decreased. These respiratory alterations resulted in significant (P less than 0.05) increases in the arterial partial pressure of carbon dioxide and decreases in pH and the partial pressure of oxygen. On the basis of cardiopulmonary function, etomidate offered rapid, safe, short duration anesthesia superior to that of thiamylal sodium."}
{"id": "PMID:464357", "title": "Serum and urine inorganic fluoride concentrations and urine oxalate concentrations following methoxyflurane anesthesia in the dog.", "content": "Plasma fluoride, urine fluoride and urine oxalate concentrations were measured before administering an anesthetic to 8 dogs, and at 0, 3, 9, 24, 48, and 72 hours following 1.5 hours of anesthesia with 1% methoxyflurane. Plasma and urine osmolalities were measured and compared with fluoride and oxalate values. Fluoride concentration increased in both plasma and urine following anesthesia when compared with the preanesthetic concentrations. Maximum mean plasma inorganic fluoride was 106.71 mumoles per liter (+/- 25.44 SE) at 9 hours after exposure to methoxyflurane was completed. By 72 hours after exposure to methoxyflurane the plasma fluoride concentration was 23.47 microM/L (+/- 5.74 SE). Mean urine inorganic fluoride concentration was highest at 9 hours after exposure to methoxyflurane and reached 6047.03 microM/L (+/- 1378.46 SE) as compared to the mean preanesthetic base-line concentration of 542.68 microM/L (+/- 132.93 SE), and the 72 hour mean urine fluoride concentration which was 1593.78 microM/L (+/- 579.46 SE). Urine oxalate concentrations, when compared with urine osmolality (mg/mOsm), increased throughout the study. The 72-hour concentration after exposure to methoxyflurane was 2.5 times the preanesthetic (mg/mOsm) oxalate concentration. Plasma osmolality did not change markedly during the study. Urine osmolalities varied between animals and collection times, but a consistent pattern did not occur. Clinical and laboratory signs of renal dysfunction were not observed in any animal during the study.", "contents": "Serum and urine inorganic fluoride concentrations and urine oxalate concentrations following methoxyflurane anesthesia in the dog. Plasma fluoride, urine fluoride and urine oxalate concentrations were measured before administering an anesthetic to 8 dogs, and at 0, 3, 9, 24, 48, and 72 hours following 1.5 hours of anesthesia with 1% methoxyflurane. Plasma and urine osmolalities were measured and compared with fluoride and oxalate values. Fluoride concentration increased in both plasma and urine following anesthesia when compared with the preanesthetic concentrations. Maximum mean plasma inorganic fluoride was 106.71 mumoles per liter (+/- 25.44 SE) at 9 hours after exposure to methoxyflurane was completed. By 72 hours after exposure to methoxyflurane the plasma fluoride concentration was 23.47 microM/L (+/- 5.74 SE). Mean urine inorganic fluoride concentration was highest at 9 hours after exposure to methoxyflurane and reached 6047.03 microM/L (+/- 1378.46 SE) as compared to the mean preanesthetic base-line concentration of 542.68 microM/L (+/- 132.93 SE), and the 72 hour mean urine fluoride concentration which was 1593.78 microM/L (+/- 579.46 SE). Urine oxalate concentrations, when compared with urine osmolality (mg/mOsm), increased throughout the study. The 72-hour concentration after exposure to methoxyflurane was 2.5 times the preanesthetic (mg/mOsm) oxalate concentration. Plasma osmolality did not change markedly during the study. Urine osmolalities varied between animals and collection times, but a consistent pattern did not occur. Clinical and laboratory signs of renal dysfunction were not observed in any animal during the study."}
{"id": "PMID:464358", "title": "Efficacy of an inactivated virus vaccine for prevention of porcine parvovirus-induced reproductive failure.", "content": "Gilts vaccinated IM either once (4 gilts) or twice (2 gilts) with an acetylethyleneimine-inactivated porcine parvovirus (PPV) vaccine before they were bred were subsequently exposed intranasally and orally to virulent PPV at about the 40th day of gestation (from 37 to 43 days). At 2 weeks after vaccination, all had hemagglutination-inhibiting (HI) titers for PPV (from 20 to 80) which decreased by the time the immunity was challenged with virulent virus (from 10 to 40), but increased thereafter (from 160 to 1,280). Titers of singly and doubly vaccinated gilts were similar throughout the experiment. The gilts were killed at about the 84th day of gestation (from 80 to 87 days), and their litters were examined. Litters were comprised of 68 live fetuses and 1 dead fetus (7 to 14 fetuses/litter). Neither viral antigen, PPV, nor homologous HI antibody was found in any of the fetuses. In addition, 4 gilts were kept in contact with the vaccinated gilts and were treated similarly except for vaccination. These 4 gilts remained free of HI antibody until after they were exposed to virulent PPV during gestation. At the time the gilts were killed the titers were 1,280 to 2,560. Their litters were comprised of 11 live fetuses and 26 dead fetuses (8 to 11 fetuses/litter). Virus was isolated from fetuses of all litters. Viral antigen was found in 24 of the dead fetuses and 10 of the live fetuses. All infected live fetuses also had HI antibody for PPV. The 2 boars used to breed vaccinated and nonvaccinated gilts (usually each gilt was bred to each of the 2 boars), but not exposed to virulent PPV, remained free of HI antibody for PPV.", "contents": "Efficacy of an inactivated virus vaccine for prevention of porcine parvovirus-induced reproductive failure. Gilts vaccinated IM either once (4 gilts) or twice (2 gilts) with an acetylethyleneimine-inactivated porcine parvovirus (PPV) vaccine before they were bred were subsequently exposed intranasally and orally to virulent PPV at about the 40th day of gestation (from 37 to 43 days). At 2 weeks after vaccination, all had hemagglutination-inhibiting (HI) titers for PPV (from 20 to 80) which decreased by the time the immunity was challenged with virulent virus (from 10 to 40), but increased thereafter (from 160 to 1,280). Titers of singly and doubly vaccinated gilts were similar throughout the experiment. The gilts were killed at about the 84th day of gestation (from 80 to 87 days), and their litters were examined. Litters were comprised of 68 live fetuses and 1 dead fetus (7 to 14 fetuses/litter). Neither viral antigen, PPV, nor homologous HI antibody was found in any of the fetuses. In addition, 4 gilts were kept in contact with the vaccinated gilts and were treated similarly except for vaccination. These 4 gilts remained free of HI antibody until after they were exposed to virulent PPV during gestation. At the time the gilts were killed the titers were 1,280 to 2,560. Their litters were comprised of 11 live fetuses and 26 dead fetuses (8 to 11 fetuses/litter). Virus was isolated from fetuses of all litters. Viral antigen was found in 24 of the dead fetuses and 10 of the live fetuses. All infected live fetuses also had HI antibody for PPV. The 2 boars used to breed vaccinated and nonvaccinated gilts (usually each gilt was bred to each of the 2 boars), but not exposed to virulent PPV, remained free of HI antibody for PPV."}
{"id": "PMID:464359", "title": "Relative importance of winter survival of larval nematodes in pasture and infected carrier calves in a study of parasitic gastroenteritis in calves.", "content": "A study was done in Maine to determine the relative importance of winter survival of nematode larvae in pasture and infected carrier animals as sources of infection for susceptible calves. Under the conditions of the experiment, it appeared that winter survivals in pasture of the infective stages of the genera Ostertagia, Cooperia, Nematodirus, and Trichostrongylus were of greater importance than carrier animals as sources of infection for susceptible calves. While animals in plots infected the previous summer and simultaneously allowed to graze alongside infected carrier animals did show more worms than those grazed only in infected plots, these differences were not statistically significant. Both groups had significantly (P greater than 0.01) more worms than did calves grazed only with carrier animals for the period of the experiment (8 weeks). It was also observed that carrier calves with low fecal egg counts (less than 200 eggs/g of feces) introduced in early spring to uncontaminated pasture could produce enough parasitic contamination by early fall to cause fulminating infections in susceptible calves grazing the pasture at the same time. Infected animals that survived clinical disease during their 1st summer developed a strong immunity which limited their acquisition of further infections when they were exposed to severe pasture contamination the following year.", "contents": "Relative importance of winter survival of larval nematodes in pasture and infected carrier calves in a study of parasitic gastroenteritis in calves. A study was done in Maine to determine the relative importance of winter survival of nematode larvae in pasture and infected carrier animals as sources of infection for susceptible calves. Under the conditions of the experiment, it appeared that winter survivals in pasture of the infective stages of the genera Ostertagia, Cooperia, Nematodirus, and Trichostrongylus were of greater importance than carrier animals as sources of infection for susceptible calves. While animals in plots infected the previous summer and simultaneously allowed to graze alongside infected carrier animals did show more worms than those grazed only in infected plots, these differences were not statistically significant. Both groups had significantly (P greater than 0.01) more worms than did calves grazed only with carrier animals for the period of the experiment (8 weeks). It was also observed that carrier calves with low fecal egg counts (less than 200 eggs/g of feces) introduced in early spring to uncontaminated pasture could produce enough parasitic contamination by early fall to cause fulminating infections in susceptible calves grazing the pasture at the same time. Infected animals that survived clinical disease during their 1st summer developed a strong immunity which limited their acquisition of further infections when they were exposed to severe pasture contamination the following year."}
{"id": "PMID:464360", "title": "Comparison of vaccination and treatment in controlling naturally occurring infectious bovine keratoconjunctivitis.", "content": "A vaccination study for infectious bovine keratoconjunctivitis was conducted on 108 newborn Hereford calves in the US Department of Agriculture Meat Animal Research Center cattle herd at Clay Center, Nebraska. Groups were allocated so that age of calf, sex of calf, and age of dam were equally distributed between the 54 vaccinated (group I) and the 54 nonvaccinated (group 2) control calves. The dams of both groups of calves were monitored as group 3 controls. An autogenous Moraxella bovis bacterin (formalin-killed, whole cells) was given IM at birth and at approximate intervals of 2 weeks for a total of 3 doses. Bacterial isolation rates for the cattle in groups 1, 2, and 3 during the summer were 92.6%, 92.6%, and 54.1%, respectively, and disease rates were 100%, 96.3%, and 70.6%. The rates were significantly (P less than 0.05) different between calves and cows. Vaccination of calves at birth permitted serum antibodies to develop before the calves were extensively exposed to infection; however, immunity to the disease did not develop. In a treatment study of other animals in the same herd, but in another pasture, the same criteria were used for allocation of 107 cow-calf pairs. Eye spray was applied to treated principals (group 4, 52 calves; and group 6, 53 cows) each week after examination and sample collection. Controls consisted of 54 calves (group 5) and 54 cows (group 7) that were examined and cultured bacteriologically in the same manner. The bacterial isolation and disease rates were less (P less than 0.05) in the treated calves (group 4) than in the nontreated controls (group 5). The differences in bacterial isolation rates between groups 6 and 7 were not significant, but group 6 had less (P less than 0.05) grade III lesions than did group 7. Weekly treatment appeared to be more effective in reducing the incidence of disease than did vaccination.", "contents": "Comparison of vaccination and treatment in controlling naturally occurring infectious bovine keratoconjunctivitis. A vaccination study for infectious bovine keratoconjunctivitis was conducted on 108 newborn Hereford calves in the US Department of Agriculture Meat Animal Research Center cattle herd at Clay Center, Nebraska. Groups were allocated so that age of calf, sex of calf, and age of dam were equally distributed between the 54 vaccinated (group I) and the 54 nonvaccinated (group 2) control calves. The dams of both groups of calves were monitored as group 3 controls. An autogenous Moraxella bovis bacterin (formalin-killed, whole cells) was given IM at birth and at approximate intervals of 2 weeks for a total of 3 doses. Bacterial isolation rates for the cattle in groups 1, 2, and 3 during the summer were 92.6%, 92.6%, and 54.1%, respectively, and disease rates were 100%, 96.3%, and 70.6%. The rates were significantly (P less than 0.05) different between calves and cows. Vaccination of calves at birth permitted serum antibodies to develop before the calves were extensively exposed to infection; however, immunity to the disease did not develop. In a treatment study of other animals in the same herd, but in another pasture, the same criteria were used for allocation of 107 cow-calf pairs. Eye spray was applied to treated principals (group 4, 52 calves; and group 6, 53 cows) each week after examination and sample collection. Controls consisted of 54 calves (group 5) and 54 cows (group 7) that were examined and cultured bacteriologically in the same manner. The bacterial isolation and disease rates were less (P less than 0.05) in the treated calves (group 4) than in the nontreated controls (group 5). The differences in bacterial isolation rates between groups 6 and 7 were not significant, but group 6 had less (P less than 0.05) grade III lesions than did group 7. Weekly treatment appeared to be more effective in reducing the incidence of disease than did vaccination."}
{"id": "PMID:464361", "title": "Distribution of glutathione peroxidase activity and selenium in the blood of dairy cows.", "content": "The distribution of glutathione peroxidase (GSH-Px) activity and selenium concentrations among several components in the blood of dairy cows was examined. Approximately 98% of the GSH-Px activity in peripheral blood was associated with the erythrocytes when enzyme activity was expressed as units per milliliter of blood. The GSH-Px activity expressed per cell was approximately fourfold greater for peripheral leukocytes than for erythrocytes. The cellular component contained a greater proportion (approx 73%) of whole blood selenium than did the plasma. A positive linear relationship (r = 0.958) between blood GSH-Px activity and blood selenium concentrations was found in dairy cattle under practical field conditions.", "contents": "Distribution of glutathione peroxidase activity and selenium in the blood of dairy cows. The distribution of glutathione peroxidase (GSH-Px) activity and selenium concentrations among several components in the blood of dairy cows was examined. Approximately 98% of the GSH-Px activity in peripheral blood was associated with the erythrocytes when enzyme activity was expressed as units per milliliter of blood. The GSH-Px activity expressed per cell was approximately fourfold greater for peripheral leukocytes than for erythrocytes. The cellular component contained a greater proportion (approx 73%) of whole blood selenium than did the plasma. A positive linear relationship (r = 0.958) between blood GSH-Px activity and blood selenium concentrations was found in dairy cattle under practical field conditions."}
{"id": "PMID:464362", "title": "Immunologic properties of soluble materials from Brucella species.", "content": "The purpose in this study was to examine the immunogenic properties of various preparations of aqueous ether extracts of Brucella suis and Brucella canis. The B suis strain 3b and B canis strain RM-6-66 were grown on tryptose agar, and aqueous ether extracts were prepared from the cells. The ether was removed, and the extracts were clarified by centrifugation for 10 hours at 144,000 X g and fractionated by gel chromatography. The B suis endotoxin-containing precipitate, obtained from aqueous ether extracted material by ethanol precipitation, and fraction 1, prepared from ultracentrifugal supernate by column chromatography, protected mice against homologous infection. The B canis aqueous ether-extracted material also protected mice against B suis infections.", "contents": "Immunologic properties of soluble materials from Brucella species. The purpose in this study was to examine the immunogenic properties of various preparations of aqueous ether extracts of Brucella suis and Brucella canis. The B suis strain 3b and B canis strain RM-6-66 were grown on tryptose agar, and aqueous ether extracts were prepared from the cells. The ether was removed, and the extracts were clarified by centrifugation for 10 hours at 144,000 X g and fractionated by gel chromatography. The B suis endotoxin-containing precipitate, obtained from aqueous ether extracted material by ethanol precipitation, and fraction 1, prepared from ultracentrifugal supernate by column chromatography, protected mice against homologous infection. The B canis aqueous ether-extracted material also protected mice against B suis infections."}
{"id": "PMID:464363", "title": "Characterization of the equine platelet aggregation response.", "content": "Equine platelet aggregation responses to bovine collagen, adenosine diphosphate (ADP), serotonin, epinephrine, and arachidonate in a platelet aggregometer were recorded. Equine platelets exhibited irreversible aggregation when incubated with ADP at a final concentration of 10 microM and bovine collagen. A secondary aggregation wave was recorded from platelets from certain horses at final ADP concentrations of 1 to 5 microM. Serotonin and arachidonate induced a weak reversible aggregation response, but a response was not observed following epinephrine addition. Equine platelet aggregation was influenced by concentration of anticoagulant (sodium citrate). Platelet aggregation responses at 37 C were indistinguishable from those recorded at 39 C. Platelet aggregation responses also were altered if the aggregation tests were not performed within 4 hours of blood sample acquisition. An assessment of platelet aggregation from multiple blood samples from the same horse indicated that the procedures described provide a reliable method to assess equine platelet aggregation in vitro.", "contents": "Characterization of the equine platelet aggregation response. Equine platelet aggregation responses to bovine collagen, adenosine diphosphate (ADP), serotonin, epinephrine, and arachidonate in a platelet aggregometer were recorded. Equine platelets exhibited irreversible aggregation when incubated with ADP at a final concentration of 10 microM and bovine collagen. A secondary aggregation wave was recorded from platelets from certain horses at final ADP concentrations of 1 to 5 microM. Serotonin and arachidonate induced a weak reversible aggregation response, but a response was not observed following epinephrine addition. Equine platelet aggregation was influenced by concentration of anticoagulant (sodium citrate). Platelet aggregation responses at 37 C were indistinguishable from those recorded at 39 C. Platelet aggregation responses also were altered if the aggregation tests were not performed within 4 hours of blood sample acquisition. An assessment of platelet aggregation from multiple blood samples from the same horse indicated that the procedures described provide a reliable method to assess equine platelet aggregation in vitro."}
{"id": "PMID:464364", "title": "Phenylbutazone inhibition of equine platelet function.", "content": "Nonsteroidal anti-inflammatory drugs impair platelet aggregation and secretion in man, pigs, and rabbits and inhibit platelet thromboxane/prostaglandin synthesis. The present investigation studied the effects of phenylbutazone on platelet aggregation and bleeding times in the horse. Aggregation responses to adenosine diphosphate and collagen were markedly impaired 15 minutes and 2 hours after treatment, but 4 hours after treatment, platelet responses approximated those prior to treatment. The in vivo effect of phenylbutazone correlated with its plasma concentrations. Phenylbutazone, like aspirin, appeared to exert its effect by inhibiting thromboxane/prostaglandin synthesis, because thrombin-induced malondialdehyde formation was inhibited. However, unlike aspirin, free arachidonate-induced malondialdehyde synthesis was reduced but not eliminated, which suggested that phenylbutazone may have more than one site of action. Although collagen-induced platelet aggregation was impaired, a response was still present, and bleeding times were not altered by phenylbutazone treatment. To account for these findings, it is proposed that equine platelets can respond to collagen by thromboxane/prostaglandin independent pathways. The physiologic and pathophysiologic importance of these findings is discussed.", "contents": "Phenylbutazone inhibition of equine platelet function. Nonsteroidal anti-inflammatory drugs impair platelet aggregation and secretion in man, pigs, and rabbits and inhibit platelet thromboxane/prostaglandin synthesis. The present investigation studied the effects of phenylbutazone on platelet aggregation and bleeding times in the horse. Aggregation responses to adenosine diphosphate and collagen were markedly impaired 15 minutes and 2 hours after treatment, but 4 hours after treatment, platelet responses approximated those prior to treatment. The in vivo effect of phenylbutazone correlated with its plasma concentrations. Phenylbutazone, like aspirin, appeared to exert its effect by inhibiting thromboxane/prostaglandin synthesis, because thrombin-induced malondialdehyde formation was inhibited. However, unlike aspirin, free arachidonate-induced malondialdehyde synthesis was reduced but not eliminated, which suggested that phenylbutazone may have more than one site of action. Although collagen-induced platelet aggregation was impaired, a response was still present, and bleeding times were not altered by phenylbutazone treatment. To account for these findings, it is proposed that equine platelets can respond to collagen by thromboxane/prostaglandin independent pathways. The physiologic and pathophysiologic importance of these findings is discussed."}
{"id": "PMID:464365", "title": "Correlations between leukocidin production and virulence of two isolates of Fusobacterium necrophorum.", "content": "Leukocidin production by Fusobacterium necrophorum was suggested to be an important element in the development of intraabdominal and liver abscesses in mice. Leukocidin production by cultures of F necrophorum was demonstrated by an in vitro assay. One of two isolates of F necrophorum was demonstrated to produce leukocidin. The leukocidin-producing strain was observed to be more infective than the nonleukocidin-producing strain (as demonstrated by abscess formation following intraperitoneal injection of immune-suppressed and normal mice). The infectivity of the leukocidin-producing strain was increased by successive passage in immune-suppressed mice. A simultaneous increase in leukocidin production was also demonstrated. The nonleukocidin-producing strain could not be passed effectively and was relatively noninfective for mice.", "contents": "Correlations between leukocidin production and virulence of two isolates of Fusobacterium necrophorum. Leukocidin production by Fusobacterium necrophorum was suggested to be an important element in the development of intraabdominal and liver abscesses in mice. Leukocidin production by cultures of F necrophorum was demonstrated by an in vitro assay. One of two isolates of F necrophorum was demonstrated to produce leukocidin. The leukocidin-producing strain was observed to be more infective than the nonleukocidin-producing strain (as demonstrated by abscess formation following intraperitoneal injection of immune-suppressed and normal mice). The infectivity of the leukocidin-producing strain was increased by successive passage in immune-suppressed mice. A simultaneous increase in leukocidin production was also demonstrated. The nonleukocidin-producing strain could not be passed effectively and was relatively noninfective for mice."}
{"id": "PMID:464366", "title": "Experimentally induced synovitis of chickens with Mycoplasma synoviae: effects of dexamethasone treatment.", "content": "Specific - pathogen - free chickens were inoculated in the right tibiometatarsal joint with a synovitis-derived Mycoplasma synoviae strain before or during dexamethasone treatment. Development of synovitis in chickens inoculated during the drug treatment was apparently delayed in comparison with development of synovitis in non-treated chickens. Severity of clinical synovitis in chickens inoculated before the drug was given was apparently less than that in chickens not treated or in chickens treated with dexamethasone. Histopathologic changes in the early stage of the infection (1 to 2 weeks) were not modified by dexamethasone treatment, although those changes in the succeeding stage (6 to 7 weeks) were greatly lessened. A relationship was observed between the dosage of dexamethasone and the severity of synovitis, as well as the kinds of cells that infiltrated into the joint lesions. Although serum antibody titers in chickens treated with an excessive dose of dexamethasone were markedly lower, clinical, bacteriologic, and histopathologic observations in chickens treated with dexamethasone were similar to those previously found in surgically thymectomized chickens. These results may support the theory that multiple synovitis of chickens caused by M synoviae infection develops mainly because of an immune response, especially by thymus-dependent functions.", "contents": "Experimentally induced synovitis of chickens with Mycoplasma synoviae: effects of dexamethasone treatment. Specific - pathogen - free chickens were inoculated in the right tibiometatarsal joint with a synovitis-derived Mycoplasma synoviae strain before or during dexamethasone treatment. Development of synovitis in chickens inoculated during the drug treatment was apparently delayed in comparison with development of synovitis in non-treated chickens. Severity of clinical synovitis in chickens inoculated before the drug was given was apparently less than that in chickens not treated or in chickens treated with dexamethasone. Histopathologic changes in the early stage of the infection (1 to 2 weeks) were not modified by dexamethasone treatment, although those changes in the succeeding stage (6 to 7 weeks) were greatly lessened. A relationship was observed between the dosage of dexamethasone and the severity of synovitis, as well as the kinds of cells that infiltrated into the joint lesions. Although serum antibody titers in chickens treated with an excessive dose of dexamethasone were markedly lower, clinical, bacteriologic, and histopathologic observations in chickens treated with dexamethasone were similar to those previously found in surgically thymectomized chickens. These results may support the theory that multiple synovitis of chickens caused by M synoviae infection develops mainly because of an immune response, especially by thymus-dependent functions."}
{"id": "PMID:464367", "title": "Evaluation of a cuprimyxin-hydrocortisone acetate suspension in the treatment of otitis externa in dogs and cats.", "content": "The therapeutic efficacy of a 0.5% cuprimyxin plus 1% hydrocortisone acetate-water-base suspension was evaluated in 130 clinical cases of otitis externa in dogs and cats in various geographic areas of the United States. The otic microbial flora was studied before treatment in 125 (96%) of the dogs and cats, with multiple isolates recovered from mated from 70%, yeasts from 65%, and fungi from 5% of the animals. Evens though multiple drug reistance was frequently observed in the bacterial flora, all bacteria and yeast isolates were sensitive to cuprimyxin. Of the 130 treated animals, a statistically significant (P less than 0.01) favorable response was obtained in 84,6% and no response in 13;1%; in2.3%, the condition became worse. An adverse reaction (pain at the time of first application) was observed in 1 (0.8%) animal.", "contents": "Evaluation of a cuprimyxin-hydrocortisone acetate suspension in the treatment of otitis externa in dogs and cats. The therapeutic efficacy of a 0.5% cuprimyxin plus 1% hydrocortisone acetate-water-base suspension was evaluated in 130 clinical cases of otitis externa in dogs and cats in various geographic areas of the United States. The otic microbial flora was studied before treatment in 125 (96%) of the dogs and cats, with multiple isolates recovered from mated from 70%, yeasts from 65%, and fungi from 5% of the animals. Evens though multiple drug reistance was frequently observed in the bacterial flora, all bacteria and yeast isolates were sensitive to cuprimyxin. Of the 130 treated animals, a statistically significant (P less than 0.01) favorable response was obtained in 84,6% and no response in 13;1%; in2.3%, the condition became worse. An adverse reaction (pain at the time of first application) was observed in 1 (0.8%) animal."}
{"id": "PMID:464368", "title": "Effects of hyperparathyroidism and dietary calcium supplementation on bone healing.", "content": "Effects of nutritional secondary hyperparathyroidism and dietary calcium supplementation on bone healing were determined. Groups (n = 4) of 5 mature male dogs each were fed the following diets: group 1, control diet (0.48% Ca, 0.43% P); group 2, test diet (0.12% Ca, 1.14% P): group 3, control diet plus calcium; group 4, test diet plus calcium. The dietary calcium supplementation was calcium gluconate. Lesions were induced in the right tibial cortex by trephinization. Within the time limitations of this study, it was determined that nutritional secondary hyperparathyroidism does not inhibit bone healing and that dietary calcium supplementation does not aid bone healing.", "contents": "Effects of hyperparathyroidism and dietary calcium supplementation on bone healing. Effects of nutritional secondary hyperparathyroidism and dietary calcium supplementation on bone healing were determined. Groups (n = 4) of 5 mature male dogs each were fed the following diets: group 1, control diet (0.48% Ca, 0.43% P); group 2, test diet (0.12% Ca, 1.14% P): group 3, control diet plus calcium; group 4, test diet plus calcium. The dietary calcium supplementation was calcium gluconate. Lesions were induced in the right tibial cortex by trephinization. Within the time limitations of this study, it was determined that nutritional secondary hyperparathyroidism does not inhibit bone healing and that dietary calcium supplementation does not aid bone healing."}
{"id": "PMID:464369", "title": "Studies on bone-remodeling rates on the Beagle: A comparison between similar biopsy sites on different ribs.", "content": "Two male littermate standardized colony-raised Beagles were studied to find out whether there was any statistically significant difference in the measurements of bone-remodeling determinations at similar sites on different ribs of the animal. Tetracycline fluorescent-labeling studies were performed on these animals, the midportion of the proximal, midshaft, and distal thirds being sampled from the 5th, 7th, 9th, and 11th ribs. Statistical analysis of the findings indicates that there is no significant difference in bone-remodeling activity between similar sites on alternate ribs. This fact permits studies on bone-remodeling activity in an animal to be done in which the same animal is used as its own control and in which more than two experimental biopsy samples are required.", "contents": "Studies on bone-remodeling rates on the Beagle: A comparison between similar biopsy sites on different ribs. Two male littermate standardized colony-raised Beagles were studied to find out whether there was any statistically significant difference in the measurements of bone-remodeling determinations at similar sites on different ribs of the animal. Tetracycline fluorescent-labeling studies were performed on these animals, the midportion of the proximal, midshaft, and distal thirds being sampled from the 5th, 7th, 9th, and 11th ribs. Statistical analysis of the findings indicates that there is no significant difference in bone-remodeling activity between similar sites on alternate ribs. This fact permits studies on bone-remodeling activity in an animal to be done in which the same animal is used as its own control and in which more than two experimental biopsy samples are required."}
{"id": "PMID:464370", "title": "Dose titration of oxfendazole against common nematodes of swine.", "content": "Oxfendazole, a benzimidazole carbamate, was administered as a 0.5% feed additive to 88 pigs naturally infected with two to four nematode species. Dose rates of 1.5 mg/kg of body weight, 3.0 mg/kg, 3.75 mg/kg, 4.5 mg/kg, 6.75 mg/kg, or 9.0 mg/kg were 100% efficacious against Oesophagostomum dentatum and 99.2% to 100% effective against Ascaris suum. Dose rates of 4.5 mg/kg, 6.75 mg/kg, and 9.0 mg/kg were 92.7%, 98.9%, and 99.5% effective, respectively, against mixed populations of Metastrongylus apri and M pudendotectus. Results were variable with Trichuris suis infections. Efficacy was based on the number of nematodes recovered at necropsy. Palatability and acceptability of the feed additive were good, and adverse reactions following administration were not observed.", "contents": "Dose titration of oxfendazole against common nematodes of swine. Oxfendazole, a benzimidazole carbamate, was administered as a 0.5% feed additive to 88 pigs naturally infected with two to four nematode species. Dose rates of 1.5 mg/kg of body weight, 3.0 mg/kg, 3.75 mg/kg, 4.5 mg/kg, 6.75 mg/kg, or 9.0 mg/kg were 100% efficacious against Oesophagostomum dentatum and 99.2% to 100% effective against Ascaris suum. Dose rates of 4.5 mg/kg, 6.75 mg/kg, and 9.0 mg/kg were 92.7%, 98.9%, and 99.5% effective, respectively, against mixed populations of Metastrongylus apri and M pudendotectus. Results were variable with Trichuris suis infections. Efficacy was based on the number of nematodes recovered at necropsy. Palatability and acceptability of the feed additive were good, and adverse reactions following administration were not observed."}
{"id": "PMID:464371", "title": "Detection of arbovirus antibodies in avian sera by the complement fixation-inhibition test.", "content": "The complement fixation-inhibition (CFI) test was described for the detection of antibodies to arboviruses in bird sera. The CFI antibody present in bird sera inhibited the standard complement-fixation reaction of a reference complement-fixing antigen-antibody pair. Using reference antigens (St. Louis encephalitis, eastern equine encephalomyelitis, western equine encephalomyelitis, and yellow fever) prepared from infected mouse brains and reference antisera prepared in rabbits or horses, reproducible CFI antibody titers were obtained in artificially immunized chickens. Time-course studies on the CFI immune response in birds inoculated with live St. Louis encephalitis virus indicated that the CFI antibody was distinct from the antibody detected by the hemagglutination-inhibition test.", "contents": "Detection of arbovirus antibodies in avian sera by the complement fixation-inhibition test. The complement fixation-inhibition (CFI) test was described for the detection of antibodies to arboviruses in bird sera. The CFI antibody present in bird sera inhibited the standard complement-fixation reaction of a reference complement-fixing antigen-antibody pair. Using reference antigens (St. Louis encephalitis, eastern equine encephalomyelitis, western equine encephalomyelitis, and yellow fever) prepared from infected mouse brains and reference antisera prepared in rabbits or horses, reproducible CFI antibody titers were obtained in artificially immunized chickens. Time-course studies on the CFI immune response in birds inoculated with live St. Louis encephalitis virus indicated that the CFI antibody was distinct from the antibody detected by the hemagglutination-inhibition test."}
{"id": "PMID:464373", "title": "Activation of lung connective tissue cells in vitro.", "content": "Guinea pig lung fibroblasts \"activated\" in vitro by exposure to connective tissue-activating peptides I and III, and guinea pig tissue extracts showed enhanced glycolysis and accelerated glycosaminoglycan synthesis. Formation of hyaluronic acid, and to a lesser extent, chondroitin 4/6-sulfate was stimulated by these agents.", "contents": "Activation of lung connective tissue cells in vitro. Guinea pig lung fibroblasts \"activated\" in vitro by exposure to connective tissue-activating peptides I and III, and guinea pig tissue extracts showed enhanced glycolysis and accelerated glycosaminoglycan synthesis. Formation of hyaluronic acid, and to a lesser extent, chondroitin 4/6-sulfate was stimulated by these agents."}
{"id": "PMID:464374", "title": "Connective tissue formation by lung fibroblasts in vitro.", "content": "Guinea pig lung fibroblasts were grown in monolayer culture after enzymatic disaggregation of lung parenchyma. Light and electron microscopy indicated that the cultures were relatively homogeneous with respect to cell type. Synthesis of matrix glycosaminoglycans and collagen was demonstrated throughout the life of the culture. The properties of the guinea pig cell strains make these a suitable in vitro model system for studying the regulation of connective tissue metabolism of pulmonary tissue.", "contents": "Connective tissue formation by lung fibroblasts in vitro. Guinea pig lung fibroblasts were grown in monolayer culture after enzymatic disaggregation of lung parenchyma. Light and electron microscopy indicated that the cultures were relatively homogeneous with respect to cell type. Synthesis of matrix glycosaminoglycans and collagen was demonstrated throughout the life of the culture. The properties of the guinea pig cell strains make these a suitable in vitro model system for studying the regulation of connective tissue metabolism of pulmonary tissue."}
{"id": "PMID:464375", "title": "A chronic isolated tracheal segment to study airway reflexes in conscious dogs.", "content": "Airway reflexes are difficult to study in conscious animals because associated changes in ventilation alter intrathoracic airway dimensions. By studying an isolated segment of extrathoracic trachea, we have overcome this problem. In each of 2 dogs, we created surgically an isolated tracheal segment just below the larynx, sealed at one end and tapered at the other to a 3-mm opening via a skin fistula. A chronic tracheostomy was also created near the thoracic outlet. We monitored intraluminal pressure (Pseg) of the isolated segment to reflect changes in smooth muscle tone. During anesthesia, with pentobarbital, gentle mechanical stimulation of the carina, deflation of the lungs, and asphyxia for one min increased Pseg (+9 to +/- 16 cm H2O). Lung inflation and alveolar hyperventilation decreased Pseg (-9 to -16 cm H2O). Five breaths of 2 per cent histamine aerosol increased Pseg (+5 cm H2O) when resting tone was normal. We also coated lumen of the isolated segment with tantalum powder and documented roentgenologically changes in the size of the segment that reflected changes in smooth muscle tone; constriction and dilation in response to asphyxia and lung inflation, respectively, were demonstrated directly by this technique. In conscious dogs, lung inflation decreased Pseg, and carinal stimulation increased Pseg. Instillation of lidocaine hydrochloride (Xylocaine) into the isolated tracheal segment blocked cough caused by mechanical stimulation of the segment, but carinal stimulation still caused constriction of the segment under these conditions which indicated that afferent, but not effrent parasympathetic innervation of the segment had been blocked selectively. Conversely, instillation of atropine sulfate into the isolated tracheal segment blocked constriction of the segment caused by carinal stimulation, but mechanical stimulation of the segment still caused cough under these conditions, which indicated that efferent, but not afferent parasympathetic innervation of the segment had been blocked selectively. We conclude that an innervated extrathoracic tracheal segment constricts and dilates via cholinergic pathways and is suitable for the study of airway reflexes in conscious dogs.", "contents": "A chronic isolated tracheal segment to study airway reflexes in conscious dogs. Airway reflexes are difficult to study in conscious animals because associated changes in ventilation alter intrathoracic airway dimensions. By studying an isolated segment of extrathoracic trachea, we have overcome this problem. In each of 2 dogs, we created surgically an isolated tracheal segment just below the larynx, sealed at one end and tapered at the other to a 3-mm opening via a skin fistula. A chronic tracheostomy was also created near the thoracic outlet. We monitored intraluminal pressure (Pseg) of the isolated segment to reflect changes in smooth muscle tone. During anesthesia, with pentobarbital, gentle mechanical stimulation of the carina, deflation of the lungs, and asphyxia for one min increased Pseg (+9 to +/- 16 cm H2O). Lung inflation and alveolar hyperventilation decreased Pseg (-9 to -16 cm H2O). Five breaths of 2 per cent histamine aerosol increased Pseg (+5 cm H2O) when resting tone was normal. We also coated lumen of the isolated segment with tantalum powder and documented roentgenologically changes in the size of the segment that reflected changes in smooth muscle tone; constriction and dilation in response to asphyxia and lung inflation, respectively, were demonstrated directly by this technique. In conscious dogs, lung inflation decreased Pseg, and carinal stimulation increased Pseg. Instillation of lidocaine hydrochloride (Xylocaine) into the isolated tracheal segment blocked cough caused by mechanical stimulation of the segment, but carinal stimulation still caused constriction of the segment under these conditions which indicated that afferent, but not effrent parasympathetic innervation of the segment had been blocked selectively. Conversely, instillation of atropine sulfate into the isolated tracheal segment blocked constriction of the segment caused by carinal stimulation, but mechanical stimulation of the segment still caused cough under these conditions, which indicated that efferent, but not afferent parasympathetic innervation of the segment had been blocked selectively. We conclude that an innervated extrathoracic tracheal segment constricts and dilates via cholinergic pathways and is suitable for the study of airway reflexes in conscious dogs."}
{"id": "PMID:464376", "title": "Comparison of two methods of measuring tracheal mucous velocity in anesthetized beagle dogs.", "content": "Tracheal mucous velocity measurements were made in 6 beagle dogs using both Sackner's method of radiographing teflon discs and Chopra's method using a gamma camera to detect the movement of instilled labeled material. Dogs were anesthetized by intravenous injection of 30 mg of sodium pentobarbital/kg of body weight in case 1 after pretreatment with atropine and Innovar-Vet; in case 2, without the pretreatment. The mean velocities measured using the 2 techniques were significantly different in both cases: case 1, 3.7 +/- 3.3 mm/min for Sackner's methods and 7.5 +/- 3.7 mm/min for Chopra's method (p less than 0.001); case 2. 3.7 +/- 3.1 mm/min for Sackner's method, and 5.5 +/- 2.1 mm/min for Chopra's method (p less than 0.05). Systematic differences in velocities should be considered when comparing data from investigators using different techniques.", "contents": "Comparison of two methods of measuring tracheal mucous velocity in anesthetized beagle dogs. Tracheal mucous velocity measurements were made in 6 beagle dogs using both Sackner's method of radiographing teflon discs and Chopra's method using a gamma camera to detect the movement of instilled labeled material. Dogs were anesthetized by intravenous injection of 30 mg of sodium pentobarbital/kg of body weight in case 1 after pretreatment with atropine and Innovar-Vet; in case 2, without the pretreatment. The mean velocities measured using the 2 techniques were significantly different in both cases: case 1, 3.7 +/- 3.3 mm/min for Sackner's methods and 7.5 +/- 3.7 mm/min for Chopra's method (p less than 0.001); case 2. 3.7 +/- 3.1 mm/min for Sackner's method, and 5.5 +/- 2.1 mm/min for Chopra's method (p less than 0.05). Systematic differences in velocities should be considered when comparing data from investigators using different techniques."}
{"id": "PMID:464377", "title": "Reverse nitrogen gradients in the study of phase III and cardiogenic oscillations of the single-breath nitrogen test.", "content": "The slope of phase III, phase IV, the slope of phase IV, and cardiac oscillations were measured on tracings obtained by both the regular single-breath N2 test (Tech I) and by a reverse technique (Tech II) in 9 healthy volunteers. Tech II consisted of 3 consecutive vital capacities (VC) of 100% O2 followed by one VC of room air. Theoretically, this should create a reversed apicobasal N2 gradient quantitatively similar to that of Tech I. From the total lung capacity following the VC2 of air, we monitored N2 concentration continuously at the mouth during a slow expiration in a manner similar to that of the single-breath N2 test. With Tech II, it is possible to preserve phase IV and its reversed slope in the presence of an almost flat slope of phase III and markedly blunted cardiac oscillations. When compared to Tech I, the slope of phase III with Tech II decreased from 0.66+/-0.20% N2/L (mean +/-SD) to 0.19+/-0.12 (p is less than 0.001), and cardiac oscillations decreased from a mean % N2 change with each heart beat of 0.87+/-0.37 to 0.24+/-0.21 (p is less than 0.005), whereas phase IV, although reversed in direction, remained quantitatively unchanged (0.35+/-0.15 L with Tech I and 0.37+/-0.14 L with Tech II), and the slope of phase IV tended to increase (2.7+/-1.9% N2 with Tech I and 3.4+/-2.1% N2 with Tech II, p=NS). We conclude that the N2 gradients within the lungs responsible for the slope of phase III and cardiac oscillations are largely independent of the gradients that give rise to phase IV and the slope of phase IV.", "contents": "Reverse nitrogen gradients in the study of phase III and cardiogenic oscillations of the single-breath nitrogen test. The slope of phase III, phase IV, the slope of phase IV, and cardiac oscillations were measured on tracings obtained by both the regular single-breath N2 test (Tech I) and by a reverse technique (Tech II) in 9 healthy volunteers. Tech II consisted of 3 consecutive vital capacities (VC) of 100% O2 followed by one VC of room air. Theoretically, this should create a reversed apicobasal N2 gradient quantitatively similar to that of Tech I. From the total lung capacity following the VC2 of air, we monitored N2 concentration continuously at the mouth during a slow expiration in a manner similar to that of the single-breath N2 test. With Tech II, it is possible to preserve phase IV and its reversed slope in the presence of an almost flat slope of phase III and markedly blunted cardiac oscillations. When compared to Tech I, the slope of phase III with Tech II decreased from 0.66+/-0.20% N2/L (mean +/-SD) to 0.19+/-0.12 (p is less than 0.001), and cardiac oscillations decreased from a mean % N2 change with each heart beat of 0.87+/-0.37 to 0.24+/-0.21 (p is less than 0.005), whereas phase IV, although reversed in direction, remained quantitatively unchanged (0.35+/-0.15 L with Tech I and 0.37+/-0.14 L with Tech II), and the slope of phase IV tended to increase (2.7+/-1.9% N2 with Tech I and 3.4+/-2.1% N2 with Tech II, p=NS). We conclude that the N2 gradients within the lungs responsible for the slope of phase III and cardiac oscillations are largely independent of the gradients that give rise to phase IV and the slope of phase IV."}
{"id": "PMID:464378", "title": "Disseminated Mycobacterium chelonei infection: response to sulfonamides.", "content": "A disseminated infection due to Mycobacterium chelonei occurred in a hemodialysis patient after rejection of his second renal transplant. Painful subcutaneous nodules and abscesses were found on both legs. Therapy with sulfisoxaxole and kanamycin produced a prompt response twice, as did sulfisoxazole alone on 2 additional occasions, despite resistance in vitro to both drugs. Clinical relapses occurred 3 times when administration of sulfisoxazole was discontinued.", "contents": "Disseminated Mycobacterium chelonei infection: response to sulfonamides. A disseminated infection due to Mycobacterium chelonei occurred in a hemodialysis patient after rejection of his second renal transplant. Painful subcutaneous nodules and abscesses were found on both legs. Therapy with sulfisoxaxole and kanamycin produced a prompt response twice, as did sulfisoxazole alone on 2 additional occasions, despite resistance in vitro to both drugs. Clinical relapses occurred 3 times when administration of sulfisoxazole was discontinued."}
{"id": "PMID:464380", "title": "The elastic properties of lobes of excised human lungs.", "content": "Static pressure-volume curves were obtained from the upper and lower lobes of 5 excised human lungs. Comparison of the upper and lower lobe pressure-volume curves showed a small but significant difference. The upper lobes contained a greater percentage of total lobar capacity above approximately 80 per cent of the lung volume. It would thus appear that the difference in lobar elastic properties could influence ventilation distribution in normal humans.", "contents": "The elastic properties of lobes of excised human lungs. Static pressure-volume curves were obtained from the upper and lower lobes of 5 excised human lungs. Comparison of the upper and lower lobe pressure-volume curves showed a small but significant difference. The upper lobes contained a greater percentage of total lobar capacity above approximately 80 per cent of the lung volume. It would thus appear that the difference in lobar elastic properties could influence ventilation distribution in normal humans."}
{"id": "PMID:464381", "title": "Lung function in children with sickle cell anemia.", "content": "Lung volumes and expiratory flows were measured in 12 children with sickle cell anemia and 12 height-matched black control subjects. Diffusing capacity of the lung for CO, pulmonary capillary blood volume, the membrane component of diffusing capacity, arterial blood gases on breathing room air and 100 per cent O2 were measured in the subjects with sickle cell anemia. The lung volumes and expiratory flows of subjects with sickle cell anemia were no different from those of the control subjects. Diffusing capacity for CO was maintined in the noraml range despite the severe anemia by increases in pulmonary capillary blood volume and the membrane component of diffusing capacity. All subjects with sickle cell anemia had mild hypoxemia and abnormal increases in calculated shunt. Pulmonary function in children with sickle cell anemia appears to be determined by their race and anemia.", "contents": "Lung function in children with sickle cell anemia. Lung volumes and expiratory flows were measured in 12 children with sickle cell anemia and 12 height-matched black control subjects. Diffusing capacity of the lung for CO, pulmonary capillary blood volume, the membrane component of diffusing capacity, arterial blood gases on breathing room air and 100 per cent O2 were measured in the subjects with sickle cell anemia. The lung volumes and expiratory flows of subjects with sickle cell anemia were no different from those of the control subjects. Diffusing capacity for CO was maintined in the noraml range despite the severe anemia by increases in pulmonary capillary blood volume and the membrane component of diffusing capacity. All subjects with sickle cell anemia had mild hypoxemia and abnormal increases in calculated shunt. Pulmonary function in children with sickle cell anemia appears to be determined by their race and anemia."}
{"id": "PMID:464382", "title": "A cluster of Mycobacterium gordonae isolates from bronchoscopy specimens.", "content": "During a 2.5-year period, 52 patients at Yale-New Haven Hospital had Mycobacterium gordonae recovered from specimens obtained by suction at bronchoscopy; 2 of them also had smears positive for acid-fast bacilli. Almost all of the isolates came from patients bronchoscoped by the same physician, one of 4 who performed the procedure during that period. Only this physician added one drop of green dye, stored in a 100-ml bottle, to the cocaine used for topical anesthesia during the procedure; cultures of the dye yielded Mycobacterium gordonae. Contamination with this organism, a cause of positive acid-fast smears, may result in an initial inappropriate diagnosis of Mycobacterium tuberculosis.", "contents": "A cluster of Mycobacterium gordonae isolates from bronchoscopy specimens. During a 2.5-year period, 52 patients at Yale-New Haven Hospital had Mycobacterium gordonae recovered from specimens obtained by suction at bronchoscopy; 2 of them also had smears positive for acid-fast bacilli. Almost all of the isolates came from patients bronchoscoped by the same physician, one of 4 who performed the procedure during that period. Only this physician added one drop of green dye, stored in a 100-ml bottle, to the cocaine used for topical anesthesia during the procedure; cultures of the dye yielded Mycobacterium gordonae. Contamination with this organism, a cause of positive acid-fast smears, may result in an initial inappropriate diagnosis of Mycobacterium tuberculosis."}
{"id": "PMID:464384", "title": "The influence of lower respiratory illness in infancy and childhood and subsequent cigarette smoking on lung function in Sydney schoolchildren.", "content": "A prospective study of respiratory illness history and lung function of 10,898 school children in Sydney was begun in 1971. At the first visit, a history of previous asthma and of bronchitis before and after the first 2 years of life was obtained from a parental questionnaire, and maximal expiratory flow-volume curves were recorded. These lung function studies were repeated yearly between 1972 and 1974, at which time a history of respiratory illness during the previous 12 months and a personal smoking history were recorded, Two groups of children from a random selection of primary and secondary schools in Sydney were studied. Their respective mean ages were 8.9 yr (primary group) and 12.6 yr (high school group) in 1971. Mean values for the maximal flow at 50 percent of the forced vital capacity were lower in children with a history of bronchitis and/or asthma than those in the control group. This was true in both age groups in both sexes. No differences were found in the 0.5-s forced expiratory volume or forced vital capacity. The differences in maximal flow at 50 per cent of the forced vital capacity were present again in 1974. In 1974, the data from smokers were compared with those from nonsmokers; small differences were found. The results suggest that bronchitis in infancy and childhood as well as asthma may affect lung function as children grow, that the abnormality may not be detected by the forced expiratory volume, that the abnormality persists, and that it is possible that the abnormality is further affected by smoking.", "contents": "The influence of lower respiratory illness in infancy and childhood and subsequent cigarette smoking on lung function in Sydney schoolchildren. A prospective study of respiratory illness history and lung function of 10,898 school children in Sydney was begun in 1971. At the first visit, a history of previous asthma and of bronchitis before and after the first 2 years of life was obtained from a parental questionnaire, and maximal expiratory flow-volume curves were recorded. These lung function studies were repeated yearly between 1972 and 1974, at which time a history of respiratory illness during the previous 12 months and a personal smoking history were recorded, Two groups of children from a random selection of primary and secondary schools in Sydney were studied. Their respective mean ages were 8.9 yr (primary group) and 12.6 yr (high school group) in 1971. Mean values for the maximal flow at 50 percent of the forced vital capacity were lower in children with a history of bronchitis and/or asthma than those in the control group. This was true in both age groups in both sexes. No differences were found in the 0.5-s forced expiratory volume or forced vital capacity. The differences in maximal flow at 50 per cent of the forced vital capacity were present again in 1974. In 1974, the data from smokers were compared with those from nonsmokers; small differences were found. The results suggest that bronchitis in infancy and childhood as well as asthma may affect lung function as children grow, that the abnormality may not be detected by the forced expiratory volume, that the abnormality persists, and that it is possible that the abnormality is further affected by smoking."}
{"id": "PMID:464385", "title": "Tuberculin test conversion during repeated skin testing, associated with sensitivity to nontuberculous mycobacteria.", "content": "To determine whether repeating the tuberculin test after a brief interval might result in tuberculin conversion, we tested 213 healthy volunteers twice, 1 month apart, with 5 TU of tuberculin purified protein derivative (PPD). Three nontuberculous mycobacterial antigens (PPD-G, PPD-Y, and PPD-B) were also applied with the first tuberculin test. By widely used criteria, 14 volunteers (6.6 per cent) converted their tuberculin tests from negative to positive on the second testing. Whereas 13 of 103 subjects (12.6 per cent) with nontuberculous antigen sensitivity converted their tuberculin test to positive, only one of 110 subjects (0.9 per cent) with no known prior mycobacterial sensitivity converted to positive (P less than 0.005; x2 = 10.05). When retested with 5 TU of tuberculin PPD 6.5 months after the second test, nine of 13 converters reverted to negative. We conclude that tuberculin conversion may occur when the skin test is repeated at 1 month, and that boosting of cross-reacting mycobacterial sensitivity might have caused a portion of the conversions in this population of young, healthy midwestern volunteers. Sensitivity to Mycobacterium tuberculosis might also be boosted by tuberculin testing. Because the prevalence of sensitization by tuberculous and nontuberculous mycobacteria can be expected to vary in different populations, the significance of tuberculin conversion will also vary with the population being tested.", "contents": "Tuberculin test conversion during repeated skin testing, associated with sensitivity to nontuberculous mycobacteria. To determine whether repeating the tuberculin test after a brief interval might result in tuberculin conversion, we tested 213 healthy volunteers twice, 1 month apart, with 5 TU of tuberculin purified protein derivative (PPD). Three nontuberculous mycobacterial antigens (PPD-G, PPD-Y, and PPD-B) were also applied with the first tuberculin test. By widely used criteria, 14 volunteers (6.6 per cent) converted their tuberculin tests from negative to positive on the second testing. Whereas 13 of 103 subjects (12.6 per cent) with nontuberculous antigen sensitivity converted their tuberculin test to positive, only one of 110 subjects (0.9 per cent) with no known prior mycobacterial sensitivity converted to positive (P less than 0.005; x2 = 10.05). When retested with 5 TU of tuberculin PPD 6.5 months after the second test, nine of 13 converters reverted to negative. We conclude that tuberculin conversion may occur when the skin test is repeated at 1 month, and that boosting of cross-reacting mycobacterial sensitivity might have caused a portion of the conversions in this population of young, healthy midwestern volunteers. Sensitivity to Mycobacterium tuberculosis might also be boosted by tuberculin testing. Because the prevalence of sensitization by tuberculous and nontuberculous mycobacteria can be expected to vary in different populations, the significance of tuberculin conversion will also vary with the population being tested."}
{"id": "PMID:464386", "title": "Absence of synergism between exposure to asbestos and cigarette smoking in asbestosis.", "content": "To assess both independent and synergistic effects of exposure to asbestos and cigarette smoking on the development of asbestosis, survey data from 4 groups of workers exposed to asbestos were analyzed with multivariate statistical models. Survey methods were standardized and included for the 383 subjects a respiratory symptoms questionnaire, occupational history, physical examination, pulmonary function testing, and a chest radiograph. Exposure to asbestos and cigarette smoking were assessed by questionnaire. Synergism between the 2 exposures was not present for previously identified manifestations of asbestosis including bilateral fine crackles, clubbing, dyspnea, radiographic abnormality, decreased forced vital capacity, and decreased single-breath diffusing capacity of the lung for CO. However, additive, independent effects of these 2 exposures were present for each of these parameters.", "contents": "Absence of synergism between exposure to asbestos and cigarette smoking in asbestosis. To assess both independent and synergistic effects of exposure to asbestos and cigarette smoking on the development of asbestosis, survey data from 4 groups of workers exposed to asbestos were analyzed with multivariate statistical models. Survey methods were standardized and included for the 383 subjects a respiratory symptoms questionnaire, occupational history, physical examination, pulmonary function testing, and a chest radiograph. Exposure to asbestos and cigarette smoking were assessed by questionnaire. Synergism between the 2 exposures was not present for previously identified manifestations of asbestosis including bilateral fine crackles, clubbing, dyspnea, radiographic abnormality, decreased forced vital capacity, and decreased single-breath diffusing capacity of the lung for CO. However, additive, independent effects of these 2 exposures were present for each of these parameters."}
{"id": "PMID:464387", "title": "The management of allergic bronchopulmonary aspergillosis.", "content": "Twenty-five patients with allergic bronchopulmonary aspergillosis (ABPA) were observed for periods of 12 months to 10 years (average duration, 2.6 years) after initial therapy with prednisone, which was then tapered and discontinued unless maintained at minimal doses as required for control of asthma. Thirteen patients have had no recurrence, 4 patients did not comply with the initial regimen and could not be considered to be controlled, and 8 patients had 12 recurrent episodes of ABPA characterized by pulmonary infiltrates with no explanation other than ABPA. The exacerbations were closely correlated with sharp increases in total serum IgE, which subsequently decreased after resumption of prednisone therapy. The increase of IgE preceded the pulmonary infiltrates in 7 or 12 exacerbations. The exacerbations, characterized by increased serum IgE and pulmonary infiltrates, may be associated with minimal symptoms. Acute asthma without pulmonary infiltrates was not associated with increased IgE. Four exacerbations occurred during administration of beclomethasone diproprionate used for control of asthma, and therefore, this agent does not appear to prevent or reverse exacerbations of ABPA. Twelve exacerbations occurred in 8 persons, with 2 patients having 4 and 2 recurrences, respectively. This suggests that exacerbations are more likely to occur in certain patients. Serial measurements of total serum IgE appears to be a useful index of disease activity in ABPA. In the 4 patients who did not comply with the prednisone therapy regimen or regular physician visits, patterns of IgE changes, clinical evaluations, and chest roentgenograms were not of use in evaluation of the clinical state or progress of the patient. A treatment regimen is suggested for initial therapy and recurrences of ABPA on the basis of these observations.", "contents": "The management of allergic bronchopulmonary aspergillosis. Twenty-five patients with allergic bronchopulmonary aspergillosis (ABPA) were observed for periods of 12 months to 10 years (average duration, 2.6 years) after initial therapy with prednisone, which was then tapered and discontinued unless maintained at minimal doses as required for control of asthma. Thirteen patients have had no recurrence, 4 patients did not comply with the initial regimen and could not be considered to be controlled, and 8 patients had 12 recurrent episodes of ABPA characterized by pulmonary infiltrates with no explanation other than ABPA. The exacerbations were closely correlated with sharp increases in total serum IgE, which subsequently decreased after resumption of prednisone therapy. The increase of IgE preceded the pulmonary infiltrates in 7 or 12 exacerbations. The exacerbations, characterized by increased serum IgE and pulmonary infiltrates, may be associated with minimal symptoms. Acute asthma without pulmonary infiltrates was not associated with increased IgE. Four exacerbations occurred during administration of beclomethasone diproprionate used for control of asthma, and therefore, this agent does not appear to prevent or reverse exacerbations of ABPA. Twelve exacerbations occurred in 8 persons, with 2 patients having 4 and 2 recurrences, respectively. This suggests that exacerbations are more likely to occur in certain patients. Serial measurements of total serum IgE appears to be a useful index of disease activity in ABPA. In the 4 patients who did not comply with the prednisone therapy regimen or regular physician visits, patterns of IgE changes, clinical evaluations, and chest roentgenograms were not of use in evaluation of the clinical state or progress of the patient. A treatment regimen is suggested for initial therapy and recurrences of ABPA on the basis of these observations."}
{"id": "PMID:464388", "title": "Acute pulmonary injury in rats by nitrofurantoin and modification by vitamin E, dietary fat, and oxygen.", "content": "The subcutaneous administration of nitrofurantoin to rats caused severe pulmonary damage, characterized by edema, congestion, and hemorrhage. The acute lethality of the drug was greater in rats fed vitamin E-deficient diets high in polyunsaturated fats as compared to rats fed the NIH open-formula diet. The survival times of vitamin E-deficient rats were increased if such animals were fed diets supplemented with vitamin E and/or diets containing saturated fat (lard) for 3 weeks before administration of nitrofurantoin. The toxicity of nitrofurantoin was enhanced in both the rats deficient in vitamin E and in those given vitamin E supplements and exposed to O2-enriched atmospheres. These results, in conjunction with previous metabolic studies in vitro showing redox cycling and O2 activation in rat lung microsomes in the presence of nitrofurantoin, illustrate certain similarities with the lung-toxic herbicide, paraquat, and raise the question of whether the 2 agents may be capable of damaging lungs by a common mechanism.", "contents": "Acute pulmonary injury in rats by nitrofurantoin and modification by vitamin E, dietary fat, and oxygen. The subcutaneous administration of nitrofurantoin to rats caused severe pulmonary damage, characterized by edema, congestion, and hemorrhage. The acute lethality of the drug was greater in rats fed vitamin E-deficient diets high in polyunsaturated fats as compared to rats fed the NIH open-formula diet. The survival times of vitamin E-deficient rats were increased if such animals were fed diets supplemented with vitamin E and/or diets containing saturated fat (lard) for 3 weeks before administration of nitrofurantoin. The toxicity of nitrofurantoin was enhanced in both the rats deficient in vitamin E and in those given vitamin E supplements and exposed to O2-enriched atmospheres. These results, in conjunction with previous metabolic studies in vitro showing redox cycling and O2 activation in rat lung microsomes in the presence of nitrofurantoin, illustrate certain similarities with the lung-toxic herbicide, paraquat, and raise the question of whether the 2 agents may be capable of damaging lungs by a common mechanism."}
{"id": "PMID:464389", "title": "Percutaneous transhepatic cholangiography with a small-gauge needle: results of a two-year study in a community hospital.", "content": "Fifty patients in a community hospital underwent evaluation of the biliary system by the new small-gauge needle technique of percutaneous transhepatic cholangiography. The high success rate, ease of performance, and low complication rate attest to the worth of this procedure in the diagnosis of hepatobiliary disease.", "contents": "Percutaneous transhepatic cholangiography with a small-gauge needle: results of a two-year study in a community hospital. Fifty patients in a community hospital underwent evaluation of the biliary system by the new small-gauge needle technique of percutaneous transhepatic cholangiography. The high success rate, ease of performance, and low complication rate attest to the worth of this procedure in the diagnosis of hepatobiliary disease."}
{"id": "PMID:464390", "title": "Percutaneous transhepatic cholangiography: an experience with the Chiba needle.", "content": "Percutaneous transhepatic cholangiography (PTC) with a \"skinny\" Chiba needle identified the biliary tree in 30 of 31 patients (97%) with extrahepatic obstructive cholestasis (EHC). The method was successful in only eight of 18 patients who had cirrhosis with unexplained jaundice. The biliary tree was visualized after one or two attempts in 23 of 31 patients with EHC (74%). The success rate was significantly greater (p greater than 0.001) in those patients with EHC than in those with EHC, in whom the biliary tree was visualized in 9 of 27 (33%). Difficulties in correctly interpreting the PTC findings in four patients are described.", "contents": "Percutaneous transhepatic cholangiography: an experience with the Chiba needle. Percutaneous transhepatic cholangiography (PTC) with a \"skinny\" Chiba needle identified the biliary tree in 30 of 31 patients (97%) with extrahepatic obstructive cholestasis (EHC). The method was successful in only eight of 18 patients who had cirrhosis with unexplained jaundice. The biliary tree was visualized after one or two attempts in 23 of 31 patients with EHC (74%). The success rate was significantly greater (p greater than 0.001) in those patients with EHC than in those with EHC, in whom the biliary tree was visualized in 9 of 27 (33%). Difficulties in correctly interpreting the PTC findings in four patients are described."}
{"id": "PMID:464391", "title": "The use of ultrasonography in the diagnosis of calculous gallbladder disease.", "content": "During a 12-month period from September 1976 to September 1977, 114 patients in a community hospital had ultrasonography as part of their diagnostic work-up for suspected gallbladder disease. While 65 per cent had an additional study, such as an oral cholecystogram or intravenous cholangiogram, 35 per cent had ultrasonography as the only study to make the diagnosis. All patients in this group had laparotomy and cholecystectomy to confirm or disprove the diagnosis of calculous gallbladder disease. The overall accuracy rate of ultrasonography for calculous gallbladder disease was 90 per cent, which compares favorably with the standard oral cholecystogram. Ultrasonography has some distinct advantages in certain clinical situations such as acute cholecystitis, jaundice, pancreatitis and pregnancy. A review of our clinical experience in the everyday use of ultrasonography for calculous biliary disease has been discussed, and guidelines for the use of ultrasonography as part of the diagnostic armamentarium for gallbladder disease are presented.", "contents": "The use of ultrasonography in the diagnosis of calculous gallbladder disease. During a 12-month period from September 1976 to September 1977, 114 patients in a community hospital had ultrasonography as part of their diagnostic work-up for suspected gallbladder disease. While 65 per cent had an additional study, such as an oral cholecystogram or intravenous cholangiogram, 35 per cent had ultrasonography as the only study to make the diagnosis. All patients in this group had laparotomy and cholecystectomy to confirm or disprove the diagnosis of calculous gallbladder disease. The overall accuracy rate of ultrasonography for calculous gallbladder disease was 90 per cent, which compares favorably with the standard oral cholecystogram. Ultrasonography has some distinct advantages in certain clinical situations such as acute cholecystitis, jaundice, pancreatitis and pregnancy. A review of our clinical experience in the everyday use of ultrasonography for calculous biliary disease has been discussed, and guidelines for the use of ultrasonography as part of the diagnostic armamentarium for gallbladder disease are presented."}
{"id": "PMID:464392", "title": "Effect of portasystemic shunt on hypersplenism: clinical study in 129 patients with cirrhosis.", "content": "The effect of portasystemic shunts on hypersplenism has been evaluated in 129 patients who were operated upon. Correction of hypersplenism was obtained in 79 per cent of patients with shunting procedures without splenectomy and 90 per cent of those with splenectomy. The improvement was significant and lasting. These results confirm the beneficial effect of splenic decompression on hypersplenism after simple portasystemic shunt. On the basis of these data, we feel that a difficult splenectomy can also be avoided in patients who have cirrhosis with striking hypersplenism.", "contents": "Effect of portasystemic shunt on hypersplenism: clinical study in 129 patients with cirrhosis. The effect of portasystemic shunts on hypersplenism has been evaluated in 129 patients who were operated upon. Correction of hypersplenism was obtained in 79 per cent of patients with shunting procedures without splenectomy and 90 per cent of those with splenectomy. The improvement was significant and lasting. These results confirm the beneficial effect of splenic decompression on hypersplenism after simple portasystemic shunt. On the basis of these data, we feel that a difficult splenectomy can also be avoided in patients who have cirrhosis with striking hypersplenism."}
{"id": "PMID:464394", "title": "Acute rupture of the lateral ligaments of the ankle joint, diagnosis and surgical treatment.", "content": "A useful method for the diagnosis of acute rupture of the lateral ligament of the ankle joint is presented. Twenty-three patients with ruptures of the lateral ligament of the ankle joint were treated surgically with suture of the lateral ligaments of the ankle. Accurate preoperative diagnosis with stress films and arthrography under local anesthesia of the ankle joint is imperative. No instances of infection or other complications were observed. All patients achieved a normal range of ankle function within three to six months. Radiograhs of the injured ankle in the forced inversion position following operation showed full stability of the ankle joint. In our opinion, all ankle injuries should be examined roentgenographically with forced inversion of the foot. Tilting of the talus of at least 10 degrees must be followed by arthrography of the ankle joint under local anesthesia. If there is leakage of contrast material along the peroneal tendon sheaths and around the joint cavity, the lateral ligament of the ankle must be sutured immediately in order to regain stability of the ankle joint.", "contents": "Acute rupture of the lateral ligaments of the ankle joint, diagnosis and surgical treatment. A useful method for the diagnosis of acute rupture of the lateral ligament of the ankle joint is presented. Twenty-three patients with ruptures of the lateral ligament of the ankle joint were treated surgically with suture of the lateral ligaments of the ankle. Accurate preoperative diagnosis with stress films and arthrography under local anesthesia of the ankle joint is imperative. No instances of infection or other complications were observed. All patients achieved a normal range of ankle function within three to six months. Radiograhs of the injured ankle in the forced inversion position following operation showed full stability of the ankle joint. In our opinion, all ankle injuries should be examined roentgenographically with forced inversion of the foot. Tilting of the talus of at least 10 degrees must be followed by arthrography of the ankle joint under local anesthesia. If there is leakage of contrast material along the peroneal tendon sheaths and around the joint cavity, the lateral ligament of the ankle must be sutured immediately in order to regain stability of the ankle joint."}
{"id": "PMID:464395", "title": "Adult hypertrophic pyloric stenosis.", "content": "Adult hypertrophic pyloric stenosis is an unusual disease of obscure etiology which causes varying degrees of gastric outlet obstruction. Diagnosis by history, physical examination, and roentgenography is difficult but can be readily made with fiberoptic gastroscopy where a characteristic pyloric \"cervix sign\" is seen. All of the many operations for relief of gastric outlet obstruction have been tried with this lesion, with resection and Billroth I reanastomosis the accepted treatment. Two patients are reported with adult hypertrophic pyloric stenosis who had typical endoscopic findings. One was treated with distal gastrectomy, and the other was adequately treated by endoscopic dilatation alone. It is suggested that endoscopic dilatation is acceptable as primary therapy in suitable cases.", "contents": "Adult hypertrophic pyloric stenosis. Adult hypertrophic pyloric stenosis is an unusual disease of obscure etiology which causes varying degrees of gastric outlet obstruction. Diagnosis by history, physical examination, and roentgenography is difficult but can be readily made with fiberoptic gastroscopy where a characteristic pyloric \"cervix sign\" is seen. All of the many operations for relief of gastric outlet obstruction have been tried with this lesion, with resection and Billroth I reanastomosis the accepted treatment. Two patients are reported with adult hypertrophic pyloric stenosis who had typical endoscopic findings. One was treated with distal gastrectomy, and the other was adequately treated by endoscopic dilatation alone. It is suggested that endoscopic dilatation is acceptable as primary therapy in suitable cases."}
{"id": "PMID:464396", "title": "Two forms of trehalase activity in mice.", "content": "Trehalase activities from mouse serum and kidney have been compared on ion exchange chromatography (DEAE-cellulose and SP-Sephadex). The two activities showed a distinct behaviour. This was confirmed by polyacrylamide gel electrophoresis and thermal lability, suggesting the presence of at least two forms of the hydrolytic enzyme trehalase in mice.", "contents": "Two forms of trehalase activity in mice. Trehalase activities from mouse serum and kidney have been compared on ion exchange chromatography (DEAE-cellulose and SP-Sephadex). The two activities showed a distinct behaviour. This was confirmed by polyacrylamide gel electrophoresis and thermal lability, suggesting the presence of at least two forms of the hydrolytic enzyme trehalase in mice."}
{"id": "PMID:464397", "title": "Differential scanning calorimetry of the thermal denaturation of human serotransferrin.", "content": "The thermal denaturation of iron-free and iron human serotransferrin has been studied by differential scanning calorimetry. At pH 7.9 in 0.05M Tris, 0.1M NaHCO3 buffer, two transitions (Td = 60.1 degrees, 70.7 degrees C), at a 5 degrees C/min heating rate, were observed for iron-free serotransferrin. The respective enthalpies of denaturation were found to be 143 and 229 kcal/mol. Iron serotransferrin exhibits a single thermogram peak with Td = 83.4 degrees C and delta H = 616 +/- 15 kcal/mol (linearly extrapolated to 0 degrees C/min heating rate), at pH 7.8. An activation energy of 104 kcal/mol was computed by the procedure of Beech. A value of 112 kcal/mol was calculated from a first-order kinetics Arrhenius plot. Rate constants were determined at several temperatures from the onset temperature to Td. Denaturation temperatures and enthalpies were linearly dependent on heating rates. The thermal denaturations of iron-free and iron serotransferrins are irreversible, under the experimental conditions used. In contrast to conalbumin, thermograms of serotransferrin solutions partially saturated with ferric ions exhibit only the peaks corresponding to those obtained on separate DSC scans of iron-free and iron serotransferrin, respectively.", "contents": "Differential scanning calorimetry of the thermal denaturation of human serotransferrin. The thermal denaturation of iron-free and iron human serotransferrin has been studied by differential scanning calorimetry. At pH 7.9 in 0.05M Tris, 0.1M NaHCO3 buffer, two transitions (Td = 60.1 degrees, 70.7 degrees C), at a 5 degrees C/min heating rate, were observed for iron-free serotransferrin. The respective enthalpies of denaturation were found to be 143 and 229 kcal/mol. Iron serotransferrin exhibits a single thermogram peak with Td = 83.4 degrees C and delta H = 616 +/- 15 kcal/mol (linearly extrapolated to 0 degrees C/min heating rate), at pH 7.8. An activation energy of 104 kcal/mol was computed by the procedure of Beech. A value of 112 kcal/mol was calculated from a first-order kinetics Arrhenius plot. Rate constants were determined at several temperatures from the onset temperature to Td. Denaturation temperatures and enthalpies were linearly dependent on heating rates. The thermal denaturations of iron-free and iron serotransferrins are irreversible, under the experimental conditions used. In contrast to conalbumin, thermograms of serotransferrin solutions partially saturated with ferric ions exhibit only the peaks corresponding to those obtained on separate DSC scans of iron-free and iron serotransferrin, respectively."}
{"id": "PMID:464403", "title": "[Congenital cystic adenomatoid malformation of the lung (author's transl)].", "content": "Four cases of congenital cystic adenomatoid malformation of the lung are described. One was a stillborn, and the other three newborns, all of them surviving after lobectomy. Pathological, clinical and radiological features are discussed and compared with previously reported cases.", "contents": "[Congenital cystic adenomatoid malformation of the lung (author's transl)]. Four cases of congenital cystic adenomatoid malformation of the lung are described. One was a stillborn, and the other three newborns, all of them surviving after lobectomy. Pathological, clinical and radiological features are discussed and compared with previously reported cases."}
{"id": "PMID:464402", "title": "[Morbidity and mortality of acute renal failure in neonatal period (author's transl)].", "content": "A retrospective study of 35 newborn with acute renal failure is presented. The main causes of renal failure were neonatal hypoxia by asfixia or hemorrhagic shock (eight), congenital malformations (two) and hypertonic dehydration (25). Mortality rate was 22% including two neonates with severe congenital malformations. Sepsis was considered as the main complicating factor and often as inducer of renal failure. It was present on 55% of cases and on 75% of the deceased newborn. Cerebral injury was frequent but a follow-up study is necessary to establish the rate of neurologic sequelae. Early diagnosis and treatment of renal failure will decrease complications with improvement in prognosis. Etiological analysis of neonatal renal failure shows the need of a better health education of people and also medical control of pregnancy and perinatal period.", "contents": "[Morbidity and mortality of acute renal failure in neonatal period (author's transl)]. A retrospective study of 35 newborn with acute renal failure is presented. The main causes of renal failure were neonatal hypoxia by asfixia or hemorrhagic shock (eight), congenital malformations (two) and hypertonic dehydration (25). Mortality rate was 22% including two neonates with severe congenital malformations. Sepsis was considered as the main complicating factor and often as inducer of renal failure. It was present on 55% of cases and on 75% of the deceased newborn. Cerebral injury was frequent but a follow-up study is necessary to establish the rate of neurologic sequelae. Early diagnosis and treatment of renal failure will decrease complications with improvement in prognosis. Etiological analysis of neonatal renal failure shows the need of a better health education of people and also medical control of pregnancy and perinatal period."}
{"id": "PMID:464404", "title": "[Growing cranial fractures in infancy (author's transl)].", "content": "Seventeen cases of growing cranial fractures in infancy are presented, eleven males and six females. Seventy per cent of the cases suffered a traumatic episode before one year of life. Thirteen of the total group experienced surgical intervention in which a completely defective duramater and alterations of the leptomeninges and the subsequent cerebral cortex were encountered. Leptomeningeal cysts were found in nine patients or 70 per cent of the cases. Ventricular system revealed alterations in all cases that were studied by means of neumoencephalography or computerized axial tomography. Evolution of 15 of the cases was follow up for a period of time which varied between five months to eleven years of age. An analysis of electroencephalographical alterations and neurological sequelae is made. An uncommon form of clinical-electroencephalographic evolution is described in one of the cases.", "contents": "[Growing cranial fractures in infancy (author's transl)]. Seventeen cases of growing cranial fractures in infancy are presented, eleven males and six females. Seventy per cent of the cases suffered a traumatic episode before one year of life. Thirteen of the total group experienced surgical intervention in which a completely defective duramater and alterations of the leptomeninges and the subsequent cerebral cortex were encountered. Leptomeningeal cysts were found in nine patients or 70 per cent of the cases. Ventricular system revealed alterations in all cases that were studied by means of neumoencephalography or computerized axial tomography. Evolution of 15 of the cases was follow up for a period of time which varied between five months to eleven years of age. An analysis of electroencephalographical alterations and neurological sequelae is made. An uncommon form of clinical-electroencephalographic evolution is described in one of the cases."}
{"id": "PMID:464405", "title": "[Radiological manifestations of Meckel's diverticulum (author's transl)].", "content": "Multiple clinical and radiological manifestations of Meckel's diverticulum are commented, presenting four cases with acute abdominal manifestations and one with chronic symptomatology, which were selected from a series of nineteen cases that were surgically verified. The authors realize a critique of the radiological techniques in the literature of the suspected cases of said entity.", "contents": "[Radiological manifestations of Meckel's diverticulum (author's transl)]. Multiple clinical and radiological manifestations of Meckel's diverticulum are commented, presenting four cases with acute abdominal manifestations and one with chronic symptomatology, which were selected from a series of nineteen cases that were surgically verified. The authors realize a critique of the radiological techniques in the literature of the suspected cases of said entity."}
{"id": "PMID:464406", "title": "[Neuronal ceroid-lipofuscinosis. Diagnosis by skin biopsy. Physiopathology (author's transl)].", "content": "Authors report a case of Batten's disease (neuronal ceroid-lipofuscinosis), in its late infantile form (Jansky-Bielschowsky), so as the ability to identify the process by ultrastructural study of the skin.", "contents": "[Neuronal ceroid-lipofuscinosis. Diagnosis by skin biopsy. Physiopathology (author's transl)]. Authors report a case of Batten's disease (neuronal ceroid-lipofuscinosis), in its late infantile form (Jansky-Bielschowsky), so as the ability to identify the process by ultrastructural study of the skin."}
{"id": "PMID:464407", "title": "[Male pseudohermaphroditism due to deficit in the conversion of cholesterol to delta5 pregnenolone (author's transl)].", "content": "One patient, aged 13 months, considered phenotipically a female, was admitted with a picture of acute dehydration. Familial history (one sister dead from the same picture at the age of 18 months), clinical data (several episodes of dehydration, dark skin and mucosae, and slight abnormalities in the external genitalia), hormonal examinations (low plasmatic levels of cortisol, aldosterone, androgens and low urinary excretion of 17 hydroxycorticoids, dehydroepiandrosterone and ethiocholanolone), chromosomal examination (karyotype XY) and histological data (normal testis) suggested a diagnosis of male pseudohermaphroditism with complete feminization due to an abnormal conversion of cholesterol in delta5 pregnenolone. Late appearance of the first episode of dehydration, particularly intense cutaneous pigmentation and statural growth and bone maturation both unaffected, are some particular traits of this patient.", "contents": "[Male pseudohermaphroditism due to deficit in the conversion of cholesterol to delta5 pregnenolone (author's transl)]. One patient, aged 13 months, considered phenotipically a female, was admitted with a picture of acute dehydration. Familial history (one sister dead from the same picture at the age of 18 months), clinical data (several episodes of dehydration, dark skin and mucosae, and slight abnormalities in the external genitalia), hormonal examinations (low plasmatic levels of cortisol, aldosterone, androgens and low urinary excretion of 17 hydroxycorticoids, dehydroepiandrosterone and ethiocholanolone), chromosomal examination (karyotype XY) and histological data (normal testis) suggested a diagnosis of male pseudohermaphroditism with complete feminization due to an abnormal conversion of cholesterol in delta5 pregnenolone. Late appearance of the first episode of dehydration, particularly intense cutaneous pigmentation and statural growth and bone maturation both unaffected, are some particular traits of this patient."}
{"id": "PMID:464408", "title": "[Schwartz-Jampel syndrome. A case report and review of literature (author's transl)].", "content": "A new case with distinctive features of Schwartz-Jampel syndrome is reported, which makes the patient number 28 after reviewing world literature on the subject. Clinical, genetic, neurophysiological and pathological point of view is studied. The myotonic discharges which the patient presented while resting, did not diminish either with general anesthesia or with curarization, being this the pattern of a true myotonia.", "contents": "[Schwartz-Jampel syndrome. A case report and review of literature (author's transl)]. A new case with distinctive features of Schwartz-Jampel syndrome is reported, which makes the patient number 28 after reviewing world literature on the subject. Clinical, genetic, neurophysiological and pathological point of view is studied. The myotonic discharges which the patient presented while resting, did not diminish either with general anesthesia or with curarization, being this the pattern of a true myotonia."}
{"id": "PMID:464409", "title": "[Total colonic aganglionism (author's transl)].", "content": "Total colonic is the most frequent form of extensive aganglionism. Diagnosis is difficult, but it must be taken in mind whenever either muscoviscidosis or ileal atresia are considered. Nine cases out of a series of 319 aganglionisms seen between 1957 and 1977 are reported. Ileostomy can be lifesaving but Duhamel-Lester Martin technique must be elected as a definitive treatment. The modification proposed by the authors, carried out in three cases, can be performed as a first-choice operation in the newborn. The long term results in these three cases are compared to those of other techniques. A general review of the problem is made.", "contents": "[Total colonic aganglionism (author's transl)]. Total colonic is the most frequent form of extensive aganglionism. Diagnosis is difficult, but it must be taken in mind whenever either muscoviscidosis or ileal atresia are considered. Nine cases out of a series of 319 aganglionisms seen between 1957 and 1977 are reported. Ileostomy can be lifesaving but Duhamel-Lester Martin technique must be elected as a definitive treatment. The modification proposed by the authors, carried out in three cases, can be performed as a first-choice operation in the newborn. The long term results in these three cases are compared to those of other techniques. A general review of the problem is made."}
{"id": "PMID:464410", "title": "[Peptic ulcer in children (7 cases). Importance of fiberendoscopy (author's transl)].", "content": "The authors studied 7 cases of peptic ulcers (2 gastric and 5 duodenals) in patients whose ages ranged between 3 and 13 4/12 years. They represented 0.5% of hospital inpatients. A history of acetyl salicilic acid ingestion was present in 3 of the cases. In 2 of the cases emotional factors were associated. Initial symptoms were distributed as follows: abdominal (4), hematemesis (2) and melena (1). The clinical course was favorable for 5 of the patients; in one of them signs of bulboduodenitis became evident in the fourth month; in another one the clinical signs persisted and a new ulcer appeared 4 months after the initial diagnosis. High levels of gastrin were found in this case. The importance of gastroduodenal fiberendoscopy in infancy for the diagnosis, histological confirmation and follow-up of peptic ulcer is emphasized.", "contents": "[Peptic ulcer in children (7 cases). Importance of fiberendoscopy (author's transl)]. The authors studied 7 cases of peptic ulcers (2 gastric and 5 duodenals) in patients whose ages ranged between 3 and 13 4/12 years. They represented 0.5% of hospital inpatients. A history of acetyl salicilic acid ingestion was present in 3 of the cases. In 2 of the cases emotional factors were associated. Initial symptoms were distributed as follows: abdominal (4), hematemesis (2) and melena (1). The clinical course was favorable for 5 of the patients; in one of them signs of bulboduodenitis became evident in the fourth month; in another one the clinical signs persisted and a new ulcer appeared 4 months after the initial diagnosis. High levels of gastrin were found in this case. The importance of gastroduodenal fiberendoscopy in infancy for the diagnosis, histological confirmation and follow-up of peptic ulcer is emphasized."}
{"id": "PMID:464411", "title": "[Meckel syndrome (author's transl)].", "content": "Authors study four cases, two siblings in two different families, with several anomalies allowing to consider them as examples of the Meckel-Gr\u00fcber syndrome. A review of the recent literature about this entity is made, as well as a comparative study of the anomalie present in the published cases.", "contents": "[Meckel syndrome (author's transl)]. Authors study four cases, two siblings in two different families, with several anomalies allowing to consider them as examples of the Meckel-Gr\u00fcber syndrome. A review of the recent literature about this entity is made, as well as a comparative study of the anomalie present in the published cases."}
{"id": "PMID:464412", "title": "[Stimulation of growth hormone (HGH) secretion by physical exercise (author's transl)].", "content": "Authors study forty eight children with severe lineal growth retardation. The more frequent causes of a stunted growth were ruled out previously (systemic and chronic diseases, metabolic disorders, genetic alterations, etc.). As a screening test, physical exercise was used to detect growth hormone (HGH) deficiency. A normal response was considered when HGH levels rose more than 7,0 ng./ml. after 20 minutes of continuous exercise. In those cases in which this level was not attained. insulin-hypoglycemia alone and followed by insulin plus an arginine infusion were used as stimuli, to confirm the lack of HGH response. A correct response to the exercise was found in 81% of the cases. It is concluded that the physical exercise as HGH stimulus is a good screening test. It presents a high degree of confidence with some other qualities, mainly: little disturbances to the patients, a rapid performance, lack of unpleasant colateral effects, and the possibility that it can be carried out by sanitary non-medical personnel.", "contents": "[Stimulation of growth hormone (HGH) secretion by physical exercise (author's transl)]. Authors study forty eight children with severe lineal growth retardation. The more frequent causes of a stunted growth were ruled out previously (systemic and chronic diseases, metabolic disorders, genetic alterations, etc.). As a screening test, physical exercise was used to detect growth hormone (HGH) deficiency. A normal response was considered when HGH levels rose more than 7,0 ng./ml. after 20 minutes of continuous exercise. In those cases in which this level was not attained. insulin-hypoglycemia alone and followed by insulin plus an arginine infusion were used as stimuli, to confirm the lack of HGH response. A correct response to the exercise was found in 81% of the cases. It is concluded that the physical exercise as HGH stimulus is a good screening test. It presents a high degree of confidence with some other qualities, mainly: little disturbances to the patients, a rapid performance, lack of unpleasant colateral effects, and the possibility that it can be carried out by sanitary non-medical personnel."}
{"id": "PMID:464413", "title": "[Neonatal small left colon syndrome (author's transl)].", "content": "Two cases of neonatal intestinal obstruction, with clinical, radiological and evolutive characteristics typical of the \"neonatal small left colon syndrome\", are described. Some etiological aspects are commented upon as well as differential diagnosis, which must be done with a certain number of diseases that produce intestinal obstruction in the newborn. Authors consider of great interest the high incidence of this syndrome in children born to diabetic mothers, diagnostic value of barium enema and the usually benign clinical course with very simple therapeutic procedures.", "contents": "[Neonatal small left colon syndrome (author's transl)]. Two cases of neonatal intestinal obstruction, with clinical, radiological and evolutive characteristics typical of the \"neonatal small left colon syndrome\", are described. Some etiological aspects are commented upon as well as differential diagnosis, which must be done with a certain number of diseases that produce intestinal obstruction in the newborn. Authors consider of great interest the high incidence of this syndrome in children born to diabetic mothers, diagnostic value of barium enema and the usually benign clinical course with very simple therapeutic procedures."}
{"id": "PMID:464414", "title": "[Congenital cystic adenomatoid malformation of the lung with systemic circulation (author's transl)].", "content": "A new observation of CCAML which systemic circulation is presented. At this occasion authors update literature review published in 1973. The total number of cases reported is 78 which our case has to be added. The existence of a systemic artery has only been demonstrated previously in three patients.", "contents": "[Congenital cystic adenomatoid malformation of the lung with systemic circulation (author's transl)]. A new observation of CCAML which systemic circulation is presented. At this occasion authors update literature review published in 1973. The total number of cases reported is 78 which our case has to be added. The existence of a systemic artery has only been demonstrated previously in three patients."}
{"id": "PMID:464415", "title": "[Benign familial neonatal convulsions (author's transl)].", "content": "Newborn male with convulsive crisis from the third day of life. Analysis and complementary examination, EEG excepted, were normal. After the 40th day of life, no further crisis were observed. Clinical evolution and psychomotor development were normal. Anamnesic findings: 13 members in five generations presented new born conculsions. No psychomotor alterations in the development were observed in all of them, excepted one. An early knowledge of this clinical picture, already described in six families, may avoid dangerous clinical examinations as well as a prolonged anticonvulsive treatment. The mode of inheritance is dominant autosomial.", "contents": "[Benign familial neonatal convulsions (author's transl)]. Newborn male with convulsive crisis from the third day of life. Analysis and complementary examination, EEG excepted, were normal. After the 40th day of life, no further crisis were observed. Clinical evolution and psychomotor development were normal. Anamnesic findings: 13 members in five generations presented new born conculsions. No psychomotor alterations in the development were observed in all of them, excepted one. An early knowledge of this clinical picture, already described in six families, may avoid dangerous clinical examinations as well as a prolonged anticonvulsive treatment. The mode of inheritance is dominant autosomial."}
{"id": "PMID:464416", "title": "[\"Free\" 9p trisomy in a male child with severe mental retardation (author's transl)].", "content": "A new case of free 9p trisomy is described. The GTG banding technique and the Giemsa-11 method showed that the extra chromosome comprised precisely the 9pter leads to q12 segment. The carrier is an eleven-year-old male child with typical facial features: globulous nose, antimongoloid slant of palpebral fissures, downward turning of mouth angels and winged ears, severe mental retardation particularly regarding language and characteristic dermatoglyphics.", "contents": "[\"Free\" 9p trisomy in a male child with severe mental retardation (author's transl)]. A new case of free 9p trisomy is described. The GTG banding technique and the Giemsa-11 method showed that the extra chromosome comprised precisely the 9pter leads to q12 segment. The carrier is an eleven-year-old male child with typical facial features: globulous nose, antimongoloid slant of palpebral fissures, downward turning of mouth angels and winged ears, severe mental retardation particularly regarding language and characteristic dermatoglyphics."}
{"id": "PMID:464417", "title": "The chronic sequelae of non-A, non-B hepatitis.", "content": "Twenty-six of 388 patients (6.7%) followed prospectively after open-heart surgery developed non-A, non-B hepatitis. Of these 26, 12 had an elevated (often fluctuating) serum alanine aminotransferase (SGPT) for greater than 1 year. Liver biopsy, done in eight of 12, showed chronic active hepatitis in six and chronic persistent hepatitis in two; one patient with chronic active hepatitis had early cirrhosis. Anicteric patients with peak SGPT greater then 300 IU/L were at greatest risk of developing chronic hepatitis. Chronic non-A, non-B hepatitis was symptomatically mild and unaccompanied by physical signs or laboratory evidence of autoimmune disease or severe chronic liver disease. In all 12 patients there was spontaneous improvement in serum transaminase over a period of 1 to 3 years, and four patients had sustained normalization of SGPT. Thus chronic active hepatitis is a common sequela of acute non-A, non-B hepatitis but may have a better prognosis than chronic active hepatitis of other causes.", "contents": "The chronic sequelae of non-A, non-B hepatitis. Twenty-six of 388 patients (6.7%) followed prospectively after open-heart surgery developed non-A, non-B hepatitis. Of these 26, 12 had an elevated (often fluctuating) serum alanine aminotransferase (SGPT) for greater than 1 year. Liver biopsy, done in eight of 12, showed chronic active hepatitis in six and chronic persistent hepatitis in two; one patient with chronic active hepatitis had early cirrhosis. Anicteric patients with peak SGPT greater then 300 IU/L were at greatest risk of developing chronic hepatitis. Chronic non-A, non-B hepatitis was symptomatically mild and unaccompanied by physical signs or laboratory evidence of autoimmune disease or severe chronic liver disease. In all 12 patients there was spontaneous improvement in serum transaminase over a period of 1 to 3 years, and four patients had sustained normalization of SGPT. Thus chronic active hepatitis is a common sequela of acute non-A, non-B hepatitis but may have a better prognosis than chronic active hepatitis of other causes."}
{"id": "PMID:464418", "title": "Knowledge and use of placebos by house officers and nurses.", "content": "Sixty house officers and 39 registered nurses in a university teaching hospital were surveyed to ascertain their knowledge of placebo action and their patterns of placebo use. The majority of physicians and nurses greatly underestimated the percentage of patients who experience pain relief when given placebo. Placebos typically were given to disliked patients who were suspected of exaggerating their pain or had failed to respond to usual medical regimens, or both. Positive responses to placebo medication were then interpreted by the physicians as evidence that the pain had no physiologic basis. Many studies have shown that overdemanding and complaining patients are, if anything, less likely to respond to placebo than patients well liked by the hospital staff. Nevertheless the results of our survey suggest that this is precisely the type of patient \"at risk\" for placebo treatment.", "contents": "Knowledge and use of placebos by house officers and nurses. Sixty house officers and 39 registered nurses in a university teaching hospital were surveyed to ascertain their knowledge of placebo action and their patterns of placebo use. The majority of physicians and nurses greatly underestimated the percentage of patients who experience pain relief when given placebo. Placebos typically were given to disliked patients who were suspected of exaggerating their pain or had failed to respond to usual medical regimens, or both. Positive responses to placebo medication were then interpreted by the physicians as evidence that the pain had no physiologic basis. Many studies have shown that overdemanding and complaining patients are, if anything, less likely to respond to placebo than patients well liked by the hospital staff. Nevertheless the results of our survey suggest that this is precisely the type of patient \"at risk\" for placebo treatment."}
{"id": "PMID:464436", "title": "Serum triiodothyronine and other clinical and laboratory indices of alcoholic liver disease.", "content": "Admission serum triiodothyronine (T3) values in 124 patients hospitalized for alcoholic liver disease were correlated with clinical and laboratory indices of liver function and commonly used determinants of thyroid function. Patients with low admission serum T3 levels had significant alterations in serum albumin, bilirubin, prothrombin time, and alkaline phosphatase associated with clinical signs of portal hypertension and collateral circulation, with little difference in serum glutamic-oxaloacetic transaminase, serum gamma glutamyl transpeptidase, or serum ornithine carbamyl transferase. This group also had a significant decrease in free T3 index despite an increase in T3 uptake; the slight reduction in total thyroxine (T4) was associated with an increase in free T4 index and no change in serum thyrotropin (TSH). For patients with alcoholic liver disease, low admission serum T3 and free T3 index values when accompanied by normal serum T4, free T4 index, and TSH levels appear to be indicative of severe liver dysfunction and increased mortality risk.", "contents": "Serum triiodothyronine and other clinical and laboratory indices of alcoholic liver disease. Admission serum triiodothyronine (T3) values in 124 patients hospitalized for alcoholic liver disease were correlated with clinical and laboratory indices of liver function and commonly used determinants of thyroid function. Patients with low admission serum T3 levels had significant alterations in serum albumin, bilirubin, prothrombin time, and alkaline phosphatase associated with clinical signs of portal hypertension and collateral circulation, with little difference in serum glutamic-oxaloacetic transaminase, serum gamma glutamyl transpeptidase, or serum ornithine carbamyl transferase. This group also had a significant decrease in free T3 index despite an increase in T3 uptake; the slight reduction in total thyroxine (T4) was associated with an increase in free T4 index and no change in serum thyrotropin (TSH). For patients with alcoholic liver disease, low admission serum T3 and free T3 index values when accompanied by normal serum T4, free T4 index, and TSH levels appear to be indicative of severe liver dysfunction and increased mortality risk."}
{"id": "PMID:464448", "title": "Ultraviolet phototherapy of uremic pruritus. Long-term results and possible mechanism of action.", "content": "The beneficial effect of sunburn-spectrum ultraviolet (UVB) phototherapy on uremic pruritus was studied. Seven patients were treated twice weekly for 4 weeks with UVB to one half of the body and placebo phototherapy to the other half. All patients noted generalized improvement without localization of benefit to the UVB side, suggesting a systemic effect of UVB. A comparison of three schedules varying from one to three treatments weekly showed that the percentage of patients responding was not influenced by frequency of UVB exposure, although patients treated more intensively improved faster. In three patients, improvement was delayed until 2 weeks after completion of a course of six treatments over 2 weeks, indicating a delayed onset of benefit in at least some patients. Overall 32 of 38 patients improved after a course of six or eight UVB exposures. Pruritus has recurred in 15 patients after a mean remission of 3 months. Sixteen patients are known to remain in remission for a mean of at least 10.6 months after the first or second course of treatment. The present evidence indicates a systemic mechanism of action for the long-lasting relief of uremic pruritus afforded by UVB phototherapy.", "contents": "Ultraviolet phototherapy of uremic pruritus. Long-term results and possible mechanism of action. The beneficial effect of sunburn-spectrum ultraviolet (UVB) phototherapy on uremic pruritus was studied. Seven patients were treated twice weekly for 4 weeks with UVB to one half of the body and placebo phototherapy to the other half. All patients noted generalized improvement without localization of benefit to the UVB side, suggesting a systemic effect of UVB. A comparison of three schedules varying from one to three treatments weekly showed that the percentage of patients responding was not influenced by frequency of UVB exposure, although patients treated more intensively improved faster. In three patients, improvement was delayed until 2 weeks after completion of a course of six treatments over 2 weeks, indicating a delayed onset of benefit in at least some patients. Overall 32 of 38 patients improved after a course of six or eight UVB exposures. Pruritus has recurred in 15 patients after a mean remission of 3 months. Sixteen patients are known to remain in remission for a mean of at least 10.6 months after the first or second course of treatment. The present evidence indicates a systemic mechanism of action for the long-lasting relief of uremic pruritus afforded by UVB phototherapy."}
{"id": "PMID:464449", "title": "Amyloidosis in chronic heroin addicts with the nephrotic syndrome.", "content": "Four chronic heroin addicts had renal amyloidosis and the nephrotic syndrome. Amyloidosis had the secondary type of tissue distribution and developed after many years of addiction. That chronic immunologic stimulation by one or more exogenous antigens or multiple acute inflammatory episodes were important factors in the pathogenesis of amyloidosis in these patients is postulated. Amyloidosis, although uncommon, must be considered among the different types of renal involvement that are known to occur in drug addicts, especially those with the nephrotic syndrome, and amyloid should be specifically searched for in their renal biopsies.", "contents": "Amyloidosis in chronic heroin addicts with the nephrotic syndrome. Four chronic heroin addicts had renal amyloidosis and the nephrotic syndrome. Amyloidosis had the secondary type of tissue distribution and developed after many years of addiction. That chronic immunologic stimulation by one or more exogenous antigens or multiple acute inflammatory episodes were important factors in the pathogenesis of amyloidosis in these patients is postulated. Amyloidosis, although uncommon, must be considered among the different types of renal involvement that are known to occur in drug addicts, especially those with the nephrotic syndrome, and amyloid should be specifically searched for in their renal biopsies."}
{"id": "PMID:464450", "title": "Immunopathologic studies of pneumonitis in systemic lupus erythematosus.", "content": "We performed immunohistopathologic studies on biopsied lung tissue obtained from two patients with lupus pneumonitis using immunofluorescence, immunoperoxidase, electron microscopy, and acid-microelution. In both patients, immunofluorescence showed granular deposits of IgG, the third component of complement (C3), and DNA in the alveolar walls. The immunoperoxidase technique in both and electron microscopy in one showed that these deposits were in the interstitium of the alveolar walls and in the alveolar capillary walls. The eluates obtained from cryostat sections of the biopsied lungs contained antinuclear factor of IgG class in one patient and showed anti-DNA antibody activity in both. We suggest that the deposits are immune complexes composed of DNA, anti-DNA antibody, and complement and that deposits of DNA-anti-DNA immune complex may play a role in lupus pneumonitis.", "contents": "Immunopathologic studies of pneumonitis in systemic lupus erythematosus. We performed immunohistopathologic studies on biopsied lung tissue obtained from two patients with lupus pneumonitis using immunofluorescence, immunoperoxidase, electron microscopy, and acid-microelution. In both patients, immunofluorescence showed granular deposits of IgG, the third component of complement (C3), and DNA in the alveolar walls. The immunoperoxidase technique in both and electron microscopy in one showed that these deposits were in the interstitium of the alveolar walls and in the alveolar capillary walls. The eluates obtained from cryostat sections of the biopsied lungs contained antinuclear factor of IgG class in one patient and showed anti-DNA antibody activity in both. We suggest that the deposits are immune complexes composed of DNA, anti-DNA antibody, and complement and that deposits of DNA-anti-DNA immune complex may play a role in lupus pneumonitis."}
{"id": "PMID:464451", "title": "Racemic warfarin and trimethoprim-sulfamethoxazole interaction in humans.", "content": "Eleven normal humans were studied to evaluate the reported interaction of racemic sodium warfarin and trimethoprim-sulfamethoxazole prospectively. Single oral doses of racemic warfarin, 1.5 mg/kg of body weight, were administered with and without 320 mg of trimethoprim and 1600 mg of sulfamethoxazole orally, beginning 7 d before the warfarin and continuing daily throughout the hypoprothrombinemia. Daily plasma samples were analyzed for one-stage prothrombin activity (Quick) and for warfarin content by high-pressure liquid chromatography. Transient cutaneous reactions developed in four of 11 subjects: a morbilliform rash in three (studies discontinued) and generalized pruritus in one. A highly significant augmentation of the warfarin effect on the mean one-stage prothrombin activity (P less than 0.03) occurred with trimethoprim-sulfamethoxazole, but no significant effect was found on the warfarin half-life (P greater than 0.5). It is concluded that trimethoprim-sulfamethoxazole interacts with racemic warfarin, possibly at a receptor-site locus.", "contents": "Racemic warfarin and trimethoprim-sulfamethoxazole interaction in humans. Eleven normal humans were studied to evaluate the reported interaction of racemic sodium warfarin and trimethoprim-sulfamethoxazole prospectively. Single oral doses of racemic warfarin, 1.5 mg/kg of body weight, were administered with and without 320 mg of trimethoprim and 1600 mg of sulfamethoxazole orally, beginning 7 d before the warfarin and continuing daily throughout the hypoprothrombinemia. Daily plasma samples were analyzed for one-stage prothrombin activity (Quick) and for warfarin content by high-pressure liquid chromatography. Transient cutaneous reactions developed in four of 11 subjects: a morbilliform rash in three (studies discontinued) and generalized pruritus in one. A highly significant augmentation of the warfarin effect on the mean one-stage prothrombin activity (P less than 0.03) occurred with trimethoprim-sulfamethoxazole, but no significant effect was found on the warfarin half-life (P greater than 0.5). It is concluded that trimethoprim-sulfamethoxazole interacts with racemic warfarin, possibly at a receptor-site locus."}
{"id": "PMID:464452", "title": "Primary familial bilateral carpal tunnel syndrome.", "content": "We identified bilateral carpal tunnel syndrome in 19 of 43 living persons of a nonconsanguineous family. No single common etiologic feature was seen. Sixty-three percent of the afflicted kindred had symptomatic digital flexor tenosynovitis. Noninflammatory thickening of the flexor retinaculum or tendon sheaths, or both, was the commonest surgical finding. The 44% prevalence, early age of onset, and equal sex ratio differ from idiopathic carpal tunnel syndrome. Family pedigree suggests an inheritable disorder transmitted by an autosomal dominant gene with a high degree of penetrance.", "contents": "Primary familial bilateral carpal tunnel syndrome. We identified bilateral carpal tunnel syndrome in 19 of 43 living persons of a nonconsanguineous family. No single common etiologic feature was seen. Sixty-three percent of the afflicted kindred had symptomatic digital flexor tenosynovitis. Noninflammatory thickening of the flexor retinaculum or tendon sheaths, or both, was the commonest surgical finding. The 44% prevalence, early age of onset, and equal sex ratio differ from idiopathic carpal tunnel syndrome. Family pedigree suggests an inheritable disorder transmitted by an autosomal dominant gene with a high degree of penetrance."}
{"id": "PMID:464453", "title": "Uric acid excretion: quantitative assessment from spot, midmorning serum and urine samples.", "content": "Uric acid excretion can be measured in milligrams of urinary uric acid per decilitre of glomerular filtrate by obtaining the product of urinary uric acid and serum creatinine concentrations and dividing by the urine creatinine (all concentrations in mg/dL). In 29 normal adult men, the excretion rate in spot, midmorning samples was 0.4 +/- 0.1 (SD) mg of uric acid per decilitre of glomerular filtrate. Eight of 36 untreated gouty men excreted acid at a rate more than three standard deviations above normal. Excretion of uric acid is conveniently and physiologically assessed by this simple method.", "contents": "Uric acid excretion: quantitative assessment from spot, midmorning serum and urine samples. Uric acid excretion can be measured in milligrams of urinary uric acid per decilitre of glomerular filtrate by obtaining the product of urinary uric acid and serum creatinine concentrations and dividing by the urine creatinine (all concentrations in mg/dL). In 29 normal adult men, the excretion rate in spot, midmorning samples was 0.4 +/- 0.1 (SD) mg of uric acid per decilitre of glomerular filtrate. Eight of 36 untreated gouty men excreted acid at a rate more than three standard deviations above normal. Excretion of uric acid is conveniently and physiologically assessed by this simple method."}
{"id": "PMID:464454", "title": "Acute oliguric renal failure induced by indomethacin: possible mechanism.", "content": "A patient with compensated congestive heart failure suffered acute deterioration of her renal function and cardiac status, requiring peritoneal dialysis, in association with indomethacin therapy. Discontinuation of this inhibitor of prostaglandin synthesis led to a prompt improvement in both her renal function and cardiac status. The patient was rechallenged with indomethacin and again developed acute reduction of her glomerular filtration rate and severe volume retention, which were again totally reversed when the drug was stopped. Urinary prostaglandin E was measured by radioimmunoassay in this patient and five additional patients with congestive heart failure and prerenal azotemia. All patients were found to have elevated levels of urinary prostaglandin E. The possible role for renal prostaglandin E as a compensatory mechanism to the vasoconstrictive stimuli present in congestive heart failure is discussed. The potential danger of inhibitors of prostaglandin synthesis in patients with congestive heart failure and prerenal azotemia is emphasized.", "contents": "Acute oliguric renal failure induced by indomethacin: possible mechanism. A patient with compensated congestive heart failure suffered acute deterioration of her renal function and cardiac status, requiring peritoneal dialysis, in association with indomethacin therapy. Discontinuation of this inhibitor of prostaglandin synthesis led to a prompt improvement in both her renal function and cardiac status. The patient was rechallenged with indomethacin and again developed acute reduction of her glomerular filtration rate and severe volume retention, which were again totally reversed when the drug was stopped. Urinary prostaglandin E was measured by radioimmunoassay in this patient and five additional patients with congestive heart failure and prerenal azotemia. All patients were found to have elevated levels of urinary prostaglandin E. The possible role for renal prostaglandin E as a compensatory mechanism to the vasoconstrictive stimuli present in congestive heart failure is discussed. The potential danger of inhibitors of prostaglandin synthesis in patients with congestive heart failure and prerenal azotemia is emphasized."}
{"id": "PMID:464460", "title": "Possible nosocomial transmission of group Y Neisseria meningitidis among oncology patients.", "content": "Within 4 days, two oncology patients in the Clinical Research Unit had bacteremia causes by group Y Neisseria meningitidis. Three additional patients, identified by prevalence survey, were found to have nasopharyngeal colonization with that serogroup, compared with only one employee (not associated with patients) and no family contacts. Infected and colonized (case) patients were located in the same or adjacent rooms but did not have close contact. A comparison of host and risk factors showed no significant differences between case patients and the other (control) patients located in the same rooms. In retrospect, the index patient, who had marked sputum production but was not isolated, had unrecognized meningococcal pneumonia and probably was the hospital source of a heavy, airborne dispersal of organisms to other patients. Respiratory isolation is warranted for patients with suspected N. meningitidis infection.", "contents": "Possible nosocomial transmission of group Y Neisseria meningitidis among oncology patients. Within 4 days, two oncology patients in the Clinical Research Unit had bacteremia causes by group Y Neisseria meningitidis. Three additional patients, identified by prevalence survey, were found to have nasopharyngeal colonization with that serogroup, compared with only one employee (not associated with patients) and no family contacts. Infected and colonized (case) patients were located in the same or adjacent rooms but did not have close contact. A comparison of host and risk factors showed no significant differences between case patients and the other (control) patients located in the same rooms. In retrospect, the index patient, who had marked sputum production but was not isolated, had unrecognized meningococcal pneumonia and probably was the hospital source of a heavy, airborne dispersal of organisms to other patients. Respiratory isolation is warranted for patients with suspected N. meningitidis infection."}
{"id": "PMID:464461", "title": "Reactive hyperreninemia in renovascular hypertension after angiotensin blockage with saralasin or converting enzyme inhibitor.", "content": "Baseline plasma renin activity and responses to saralasin and converting enzyme inhibitor SQ 20881 (teprotide) in 47 untreated patients with surgically correctable renovascular hypertension were compared to those in 100 patients with high- and normal-renin essential hypertension. All 32 renovascular patients on normal sodium intake had high renin-sodium profiles and renin values greater than or equal to 5 ng angiotensin I/mL.h, as compared to 20 of 64 with essential hypertension. Diagnostic discrimination was greatly enhanced by infusion of saralasin or SQ 20881, which elicited marked reactive hyperreninemia in 31 of 32 renovascular patients but in only two of 64 with essential hypertension. Reactive hyperreninemia appeared to be more a specific test for renovascular hypertension than depressor responses. Prior dietary sodium depletion abolished this specificity. The results suggest that after initial screening with renin measurements, testing with angiotensin blocking agents may be a useful secondary screening procedure for more invasive and definitive procedures.", "contents": "Reactive hyperreninemia in renovascular hypertension after angiotensin blockage with saralasin or converting enzyme inhibitor. Baseline plasma renin activity and responses to saralasin and converting enzyme inhibitor SQ 20881 (teprotide) in 47 untreated patients with surgically correctable renovascular hypertension were compared to those in 100 patients with high- and normal-renin essential hypertension. All 32 renovascular patients on normal sodium intake had high renin-sodium profiles and renin values greater than or equal to 5 ng angiotensin I/mL.h, as compared to 20 of 64 with essential hypertension. Diagnostic discrimination was greatly enhanced by infusion of saralasin or SQ 20881, which elicited marked reactive hyperreninemia in 31 of 32 renovascular patients but in only two of 64 with essential hypertension. Reactive hyperreninemia appeared to be more a specific test for renovascular hypertension than depressor responses. Prior dietary sodium depletion abolished this specificity. The results suggest that after initial screening with renin measurements, testing with angiotensin blocking agents may be a useful secondary screening procedure for more invasive and definitive procedures."}
{"id": "PMID:464462", "title": "Ventriculoatrial intervals: diagnostic use in paroxysmal supraventricular tachycardia.", "content": "Reciprocating tachycardias due to reentry either within the atrioventricular (AV) node or using an accessory AV pathway are a common cause of paroxysmal supraventricular tachycardia in humans. Unfortunately, although of potential therapeutic value, differentiation of these forms of reciprocating tachycardia may be difficult and require detailed electrophysiologic study. To develop diagnostic criteria that permit exclusion of participation of an accessory AV pathway in reciprocating tachycardia without extensive laboratory testing, results of electrophysiologic studies were examined in 50 patients with Wolff-Parkinson-White syndrome, 15 patients with accessory AV pathways that conducted only in the ventriculoatrial direction, and 15 patients with reentry within the AV node. The interval between onset of ventricular activation and both earliest recorded atrial activity (V-Amin) and high lateral right atrial electrogram (V-HRA) was measured during tachycardia. A V-Amin of 61 ms or less or V-HRA of 95 ms or less did not occur in patients with accessory AV pathways, but occurred frequently (12 of 15 and seven or eight, respectively) in patients with reentry within the AV node. Therefore, in patients with paroxysmal reciprocating tachycardias, V-A interval measurements provide a screening test capable of excluding participation of an accessory AV pathway.", "contents": "Ventriculoatrial intervals: diagnostic use in paroxysmal supraventricular tachycardia. Reciprocating tachycardias due to reentry either within the atrioventricular (AV) node or using an accessory AV pathway are a common cause of paroxysmal supraventricular tachycardia in humans. Unfortunately, although of potential therapeutic value, differentiation of these forms of reciprocating tachycardia may be difficult and require detailed electrophysiologic study. To develop diagnostic criteria that permit exclusion of participation of an accessory AV pathway in reciprocating tachycardia without extensive laboratory testing, results of electrophysiologic studies were examined in 50 patients with Wolff-Parkinson-White syndrome, 15 patients with accessory AV pathways that conducted only in the ventriculoatrial direction, and 15 patients with reentry within the AV node. The interval between onset of ventricular activation and both earliest recorded atrial activity (V-Amin) and high lateral right atrial electrogram (V-HRA) was measured during tachycardia. A V-Amin of 61 ms or less or V-HRA of 95 ms or less did not occur in patients with accessory AV pathways, but occurred frequently (12 of 15 and seven or eight, respectively) in patients with reentry within the AV node. Therefore, in patients with paroxysmal reciprocating tachycardias, V-A interval measurements provide a screening test capable of excluding participation of an accessory AV pathway."}
{"id": "PMID:464463", "title": "Obstruction of the proximal right coronary artery with acute inferior infarction due to blunt chest trauma.", "content": "Two patients developed an acute transmural myocardial infarction due to severe obstruction of the proximal right coronary artery after blunt chest trauma. Neither had a history of ischemic heart disease, and both had an arteriographically normal left coronary artery. In one patient significant resolution of the subtotal obstruction occurred within 3 months. An intimal tear or subintimal hemorrhage with luminal thrombosis, or both, are the suggested mechanism of coronary arterial occlusion. Spasm and platelet aggregates may contribute. Despite a large number of automobile accidents, obstruction of the right coronary artery due to blunt chest trauma has not been previously described. This suggests it has been overlooked and should be especially suspected in persons with injury to the sternum and an acute inferior myocardial infarction.", "contents": "Obstruction of the proximal right coronary artery with acute inferior infarction due to blunt chest trauma. Two patients developed an acute transmural myocardial infarction due to severe obstruction of the proximal right coronary artery after blunt chest trauma. Neither had a history of ischemic heart disease, and both had an arteriographically normal left coronary artery. In one patient significant resolution of the subtotal obstruction occurred within 3 months. An intimal tear or subintimal hemorrhage with luminal thrombosis, or both, are the suggested mechanism of coronary arterial occlusion. Spasm and platelet aggregates may contribute. Despite a large number of automobile accidents, obstruction of the right coronary artery due to blunt chest trauma has not been previously described. This suggests it has been overlooked and should be especially suspected in persons with injury to the sternum and an acute inferior myocardial infarction."}
{"id": "PMID:464464", "title": "Pheochromocytoma associated with hypercalcemia and ectopic secretion of calcitonin.", "content": "A 17-year-old woman manifested fever, abdominal pain, headache, and hypertension caused by a solitary, benign pheochromocytoma. She also had hypercalcemia and elevated plasma immunoreactive calcitonin levels. After removal of the pheochromocytoma, calcium and calcitonin levels returned to normal. Studies of peripheral and tumor venous blood showed no excess or ectopic parathyroid hormone secretion, but the tumor contained and secreted calcitonin. Sporadic pheochromocytoma may secrete calcitonin and cause hypercalcemia by non-parathyroid hormone-mediated mechanisms. The potential is clearly present for confusion with multiple endocrine neoplasia, type 2 (medullary thyroid carcinoma, pheochromocytoma, and primary hyperparathyroidism).", "contents": "Pheochromocytoma associated with hypercalcemia and ectopic secretion of calcitonin. A 17-year-old woman manifested fever, abdominal pain, headache, and hypertension caused by a solitary, benign pheochromocytoma. She also had hypercalcemia and elevated plasma immunoreactive calcitonin levels. After removal of the pheochromocytoma, calcium and calcitonin levels returned to normal. Studies of peripheral and tumor venous blood showed no excess or ectopic parathyroid hormone secretion, but the tumor contained and secreted calcitonin. Sporadic pheochromocytoma may secrete calcitonin and cause hypercalcemia by non-parathyroid hormone-mediated mechanisms. The potential is clearly present for confusion with multiple endocrine neoplasia, type 2 (medullary thyroid carcinoma, pheochromocytoma, and primary hyperparathyroidism)."}
{"id": "PMID:464465", "title": "Bladder complications in patients receiving cyclophosphamide for systemic lupus erythematosus or rheumatoid arthritis.", "content": "The bladder complications of 54 patients treated with oral cyclophosphamide for systemic lupus erythematosus (43) or rheumatoid arthritis (11) were reviewed. During an observation period of 241 patient years, we saw seven cases of acute hemorrhagic cystitis and two cases of transitional cell carcinoma of the bladder. Bladder carcinoma was identified 28 and 60 months after withdrawal of the drug and resulted in the death of one patient. The late-occurring serious toxicities of cyclophosphamide should limit the use of the drug in the treatment of nonmalignant inflammatory rheumatic conditions.", "contents": "Bladder complications in patients receiving cyclophosphamide for systemic lupus erythematosus or rheumatoid arthritis. The bladder complications of 54 patients treated with oral cyclophosphamide for systemic lupus erythematosus (43) or rheumatoid arthritis (11) were reviewed. During an observation period of 241 patient years, we saw seven cases of acute hemorrhagic cystitis and two cases of transitional cell carcinoma of the bladder. Bladder carcinoma was identified 28 and 60 months after withdrawal of the drug and resulted in the death of one patient. The late-occurring serious toxicities of cyclophosphamide should limit the use of the drug in the treatment of nonmalignant inflammatory rheumatic conditions."}
{"id": "PMID:464468", "title": "The effect of a primary-care pathway on internal medicine residents' career plans.", "content": "There has been no evidence that primary-care pathways, a recent innovation in internal medicine residency programs, have affected the career choices of their trainees. We report the experience of the first four cohorts of primary-care trainees in internal medicine compared with traditional-pathway trainees at Boston City Hospital. Primary-care residents remained committed to their plans for a career in general internal medicine throughout training. In contrast, two thirds of the traditionally trained residents who were planning a career in general internal medicine at the beginning of their training changed their plans to subspecialty medicine. Thus, the primary-care pathway reinforced the career plans of trainees in general internal medicine, whereas traditional training influenced potential generalists toward subspecialty medicine.", "contents": "The effect of a primary-care pathway on internal medicine residents' career plans. There has been no evidence that primary-care pathways, a recent innovation in internal medicine residency programs, have affected the career choices of their trainees. We report the experience of the first four cohorts of primary-care trainees in internal medicine compared with traditional-pathway trainees at Boston City Hospital. Primary-care residents remained committed to their plans for a career in general internal medicine throughout training. In contrast, two thirds of the traditionally trained residents who were planning a career in general internal medicine at the beginning of their training changed their plans to subspecialty medicine. Thus, the primary-care pathway reinforced the career plans of trainees in general internal medicine, whereas traditional training influenced potential generalists toward subspecialty medicine."}
{"id": "PMID:464469", "title": "A national study of internal medicine and its specialties: II. Primary care in internal medicine.", "content": "A nationwide study of practitioners in 24 medical and surgical specialties was conducted by the University of Southern California School of Medicine, Division of Research in Medical Education. In this second report of a series presenting findings for internal medicine, general internal medicine and 10 subspecialties of internal medicine are compared using a care classification scheme designed for the study. On the basis of characteristics of the individual patient encounter, this care classification scheme distinguishes several dimensions associated with the concept of primary care. Five empirically derived types of care rather than a simple \"primary\" or \"non-primary\" dichotomy are described, and the distributions of each type for the 11 subspecialties examined are noted. Types of care (according to the care classification) are examined by the time per patient, the complexity of physician services, the severity and chronicity of problems, and the degree of specialization associated with providing different types of care. Estimates of the number of annual encounters, and the number of annual encounters for three of the five types of care, with the proportion of each accounted for by each subspecialty, are given.", "contents": "A national study of internal medicine and its specialties: II. Primary care in internal medicine. A nationwide study of practitioners in 24 medical and surgical specialties was conducted by the University of Southern California School of Medicine, Division of Research in Medical Education. In this second report of a series presenting findings for internal medicine, general internal medicine and 10 subspecialties of internal medicine are compared using a care classification scheme designed for the study. On the basis of characteristics of the individual patient encounter, this care classification scheme distinguishes several dimensions associated with the concept of primary care. Five empirically derived types of care rather than a simple \"primary\" or \"non-primary\" dichotomy are described, and the distributions of each type for the 11 subspecialties examined are noted. Types of care (according to the care classification) are examined by the time per patient, the complexity of physician services, the severity and chronicity of problems, and the degree of specialization associated with providing different types of care. Estimates of the number of annual encounters, and the number of annual encounters for three of the five types of care, with the proportion of each accounted for by each subspecialty, are given."}
{"id": "PMID:464470", "title": "National study of internal medicine manpower: III. Subspecialty fellowship training 1976-1977.", "content": "Questionnaire II of the National Study of Internal Medicine Manpower was directed to all of the 1502 subspecialty training programs in the United States and Puerto Rico. The overall response rate was 86%. For the years 1972-1973 through 1976-1977 the number of fellows in subspecialty training grew at an average rate of 10.6% per year, or one and one-half times greater than the growth rate of 7.2% in the number of first-year residents in training for the same time period. In 1976-1977 there were 5826 fellows in subspecialty fellowship training, of whom 26% were foreign medical graduates. Stipends for subspecialty fellows in 1976-1977 amounted to $90 million, 40% of which was derived from direct federal funds and 33% from hospital revenues. Most of the subspecialty fellowship programs were in large teaching hospitals, which are closely affiliated with the nation's medical schools. The 1976-1977 professional activities of former subspecialty trainees who had finished their training between 1972 and 1976 were distributed roughly in thirds between research-teaching, teaching-practice, and practice. We discuss public policy implications of the data.", "contents": "National study of internal medicine manpower: III. Subspecialty fellowship training 1976-1977. Questionnaire II of the National Study of Internal Medicine Manpower was directed to all of the 1502 subspecialty training programs in the United States and Puerto Rico. The overall response rate was 86%. For the years 1972-1973 through 1976-1977 the number of fellows in subspecialty training grew at an average rate of 10.6% per year, or one and one-half times greater than the growth rate of 7.2% in the number of first-year residents in training for the same time period. In 1976-1977 there were 5826 fellows in subspecialty fellowship training, of whom 26% were foreign medical graduates. Stipends for subspecialty fellows in 1976-1977 amounted to $90 million, 40% of which was derived from direct federal funds and 33% from hospital revenues. Most of the subspecialty fellowship programs were in large teaching hospitals, which are closely affiliated with the nation's medical schools. The 1976-1977 professional activities of former subspecialty trainees who had finished their training between 1972 and 1976 were distributed roughly in thirds between research-teaching, teaching-practice, and practice. We discuss public policy implications of the data."}
{"id": "PMID:464471", "title": "National study of internal medicine manpower: IV. Residency and fellowship training 1977-1978 and 1978-1979.", "content": "The National Study of Internal Medicine Manpower gathered data on the number of residents in training in internal medicine and the number of fellows in subspecialty training, for 1977-1978 and for 1978-1979. In the latter period, there were 16720 residents in all years of training. The 7.2% average annual increase in the number of first-year residents during the earlier half of the 1970s slowed in 1977-1978 and 1978-1979 to 4.6% and 4.2%, respectively, reflecting a similar decline in the number of medical school graduates. The most important finding of the study is that the steep rise (10.6% per year) in the number of subspecialty fellowship trainees characteristic of the years 1972-1973 through 1976-1977 has abated. The number of fellows in subspecialty training has remained essentially constant in the past 2 years. Thus, although the number of residents continued to increase and the number of fellows remained constant, the number (and percentage) of internists in training who intend to practice general internal medicine rose.", "contents": "National study of internal medicine manpower: IV. Residency and fellowship training 1977-1978 and 1978-1979. The National Study of Internal Medicine Manpower gathered data on the number of residents in training in internal medicine and the number of fellows in subspecialty training, for 1977-1978 and for 1978-1979. In the latter period, there were 16720 residents in all years of training. The 7.2% average annual increase in the number of first-year residents during the earlier half of the 1970s slowed in 1977-1978 and 1978-1979 to 4.6% and 4.2%, respectively, reflecting a similar decline in the number of medical school graduates. The most important finding of the study is that the steep rise (10.6% per year) in the number of subspecialty fellowship trainees characteristic of the years 1972-1973 through 1976-1977 has abated. The number of fellows in subspecialty training has remained essentially constant in the past 2 years. Thus, although the number of residents continued to increase and the number of fellows remained constant, the number (and percentage) of internists in training who intend to practice general internal medicine rose."}
{"id": "PMID:464501", "title": "Pigmentary paravenous chorioretinal degeneration: fundus appearance and retinal functions.", "content": "A case of pigmentary paravenous chorioretinal degeneration initially diagnosed as retinitis pigmentosa is presented. The focal macular visual evoked response (VER) was normal. Both rod and cone components in the electroretinogram showed reduced amplitude with normal peak time. The psychophysical threshold in the dark adaptation was nearly normal in some parts of the retina and extremely elevated in other parts of the retina. Fundus appearance and retinal function studies suggest that the impaired area in the retina and choroid is localized in this disease.", "contents": "Pigmentary paravenous chorioretinal degeneration: fundus appearance and retinal functions. A case of pigmentary paravenous chorioretinal degeneration initially diagnosed as retinitis pigmentosa is presented. The focal macular visual evoked response (VER) was normal. Both rod and cone components in the electroretinogram showed reduced amplitude with normal peak time. The psychophysical threshold in the dark adaptation was nearly normal in some parts of the retina and extremely elevated in other parts of the retina. Fundus appearance and retinal function studies suggest that the impaired area in the retina and choroid is localized in this disease."}
{"id": "PMID:464502", "title": "Acute retinal necrosis.", "content": "Three cases of acute retinal necrosis are presented. Acute retinal necrosis is an unusual syndrome which is the result of severe contusion to the globe. It is characterized by large irregular retinal holes with pieces of necrotic retina in the adjacent vitreous, retinal edema and hemorrhage surrounding the holes, underlying choroidal disruption, and vitreous hemorrhage. The incidence of acute retinal necrosis is difficult to determine but it does not appear to be common entity. Although acute retinal necrosis may be associated with a retinal detachment, other retinal breaks are usually responsible for the detachment. The necrosis results from the direct force of the injury striking the globe although a contrecoup mechanism may explain cases of nasal necrosis. Treatment is necessary for associated retinal detachments and acute lesions but may not be necessary if adequate fibrosis is present when the lesion is first seen.", "contents": "Acute retinal necrosis. Three cases of acute retinal necrosis are presented. Acute retinal necrosis is an unusual syndrome which is the result of severe contusion to the globe. It is characterized by large irregular retinal holes with pieces of necrotic retina in the adjacent vitreous, retinal edema and hemorrhage surrounding the holes, underlying choroidal disruption, and vitreous hemorrhage. The incidence of acute retinal necrosis is difficult to determine but it does not appear to be common entity. Although acute retinal necrosis may be associated with a retinal detachment, other retinal breaks are usually responsible for the detachment. The necrosis results from the direct force of the injury striking the globe although a contrecoup mechanism may explain cases of nasal necrosis. Treatment is necessary for associated retinal detachments and acute lesions but may not be necessary if adequate fibrosis is present when the lesion is first seen."}
{"id": "PMID:464504", "title": "Superficial keratitis in treated systemic lupus erythematosus.", "content": "A 25-year-old woman with decreased visual acuity and irritation of both eyes was found to have superficial keratitis with keratitis sicca. Her medical record indicated that she had systemic lupus erythematosus. Systemic steroids were necessary to cure the keratitis.", "contents": "Superficial keratitis in treated systemic lupus erythematosus. A 25-year-old woman with decreased visual acuity and irritation of both eyes was found to have superficial keratitis with keratitis sicca. Her medical record indicated that she had systemic lupus erythematosus. Systemic steroids were necessary to cure the keratitis."}
{"id": "PMID:464505", "title": "Retinociliary vein associated with a craniopharyngioma.", "content": "A 60-year-old white man had a diagnosis of craniopharyngioma made in 1945. Three operations for partial tumor removal were performed in 1951, 1957, and 1962. Ophthalmoscopy and fluorescein angiography revealed bilateral optic atrophy and a large retinociliary vein in the left eye. The present report is the only example of a retinociliary vein documented with photography and fluorescein angiography. It is not possible to ascertain whether this retinociliary vein is an acquired malformation or a congenital development anomaly. The extreme rarity of this entity, though, suggests that this retinociliary vein developed as a result of obstruction, by the craniopharyngioma, to the retinal venous outflow during early development.", "contents": "Retinociliary vein associated with a craniopharyngioma. A 60-year-old white man had a diagnosis of craniopharyngioma made in 1945. Three operations for partial tumor removal were performed in 1951, 1957, and 1962. Ophthalmoscopy and fluorescein angiography revealed bilateral optic atrophy and a large retinociliary vein in the left eye. The present report is the only example of a retinociliary vein documented with photography and fluorescein angiography. It is not possible to ascertain whether this retinociliary vein is an acquired malformation or a congenital development anomaly. The extreme rarity of this entity, though, suggests that this retinociliary vein developed as a result of obstruction, by the craniopharyngioma, to the retinal venous outflow during early development."}
{"id": "PMID:464507", "title": "Response of the cornea to far ultraviolet light: an ultrastructural study.", "content": "Quantitative changes were measured and ultrastructural alterations were detected in rabbit corneas after exposure to a far ultraviolet irradiation (UV). Low dose exposure (105 - 8 x 105 erg/cm2) resulted one day later in a thicker epithelium and loss of the intercellular permeability barrier. In corneas exposed to higher doses (5 x 106 erg/cm2), the epithelium was considerably thinner one day later, whereas 4 days later it was of normal thickness and the endothelial cells became degenerated. The highest UV dose exposure used (a total of 20 x 106 erg/cm2 given in 4 equal daily doses) resulted in (1) swelling of the stroma and disorganization of the collagen fibers; (2) disappearance of the basement membrane; (3) degeneration of the endothelial cells; and (4) considerable reduction of epithelium thickness.", "contents": "Response of the cornea to far ultraviolet light: an ultrastructural study. Quantitative changes were measured and ultrastructural alterations were detected in rabbit corneas after exposure to a far ultraviolet irradiation (UV). Low dose exposure (105 - 8 x 105 erg/cm2) resulted one day later in a thicker epithelium and loss of the intercellular permeability barrier. In corneas exposed to higher doses (5 x 106 erg/cm2), the epithelium was considerably thinner one day later, whereas 4 days later it was of normal thickness and the endothelial cells became degenerated. The highest UV dose exposure used (a total of 20 x 106 erg/cm2 given in 4 equal daily doses) resulted in (1) swelling of the stroma and disorganization of the collagen fibers; (2) disappearance of the basement membrane; (3) degeneration of the endothelial cells; and (4) considerable reduction of epithelium thickness."}
{"id": "PMID:464508", "title": "Spontaneous regression of a choroidal hemangioma following pregnancy.", "content": "A 30-year old white woman was seen with a 2 month history of decreased vision in the right eye. She was 5 months pregnant at this time. Examination showed a large, slightly elevated choroidal hemangioma along the superior temporal arcade. Overlying subretinal fluid involved the superior part of the macula. After the delivery of the child, the tumor regressed. This exacerbation and regression of a choroidal hemangioma during pregnancy and delivery were documented with fundus photography and fluorescein angiography.", "contents": "Spontaneous regression of a choroidal hemangioma following pregnancy. A 30-year old white woman was seen with a 2 month history of decreased vision in the right eye. She was 5 months pregnant at this time. Examination showed a large, slightly elevated choroidal hemangioma along the superior temporal arcade. Overlying subretinal fluid involved the superior part of the macula. After the delivery of the child, the tumor regressed. This exacerbation and regression of a choroidal hemangioma during pregnancy and delivery were documented with fundus photography and fluorescein angiography."}
{"id": "PMID:464509", "title": "Synchronous determination of retinal circulation times in both eyes.", "content": "Retinal circulation times of 44 healthy subjects (88 eyes) were measured simultaneously on both eyes by means of synchronous bilateral fluorescein angiography. \"Circulation time\" was defined as the time from the first arterial filling to the first fluorescence of the corresponding vein close to the disc margin. We found 1.2 sec for the superior temporal vessels, 1.3 sec for the inferior temporal, 1.3 sec for the superior nasal, and 1.4 sec for the inferior nasal vessels of the right eye. For the left eye these values were 1.2 sec, 1.2 sec, 1.4 sec, and 1.4 sec, respectively. No significant differences between the 2 eyes were demonstrable. It is concluded therefore that in cases of monocular disturbances of retinal circulation the other eye can serve as a reliable standard when measurements are performed simultaneously on both sides. The many uncertainties of successive examinations caused by uncontrollable systemic circulation changes are thus eliminated.", "contents": "Synchronous determination of retinal circulation times in both eyes. Retinal circulation times of 44 healthy subjects (88 eyes) were measured simultaneously on both eyes by means of synchronous bilateral fluorescein angiography. \"Circulation time\" was defined as the time from the first arterial filling to the first fluorescence of the corresponding vein close to the disc margin. We found 1.2 sec for the superior temporal vessels, 1.3 sec for the inferior temporal, 1.3 sec for the superior nasal, and 1.4 sec for the inferior nasal vessels of the right eye. For the left eye these values were 1.2 sec, 1.2 sec, 1.4 sec, and 1.4 sec, respectively. No significant differences between the 2 eyes were demonstrable. It is concluded therefore that in cases of monocular disturbances of retinal circulation the other eye can serve as a reliable standard when measurements are performed simultaneously on both sides. The many uncertainties of successive examinations caused by uncontrollable systemic circulation changes are thus eliminated."}
{"id": "PMID:464510", "title": "System of notation for photocoagulation in the presumed ocular histoplasmosis syndrome.", "content": "Using a notation system for evaluation of photocoagulatin of the choroidal neovascular membrane (CNVM) in presumed ocular histoplasmosis syndrome (POHS) demonstrated several advantages, which are: (1) a projector system which allowed excellent magnification of the CNVM and its distance from the foveal avascular zone (FAZ); (2) direct study of fluorescein angiographic negatives; (3) good comparison of pretreatment of posttreatment lesions in relation to FAZ; (4) a precise measurement of the surface area of each CNVM and its distance from the FAZ; and (5) an accurate and rapid way of recording and comparing data. This notation system appears valuable as a means of standardizing the examination and photographic findings from multiple institutions. Results of photocoagulatin of the CNVM are presented. Photocoagulatin of the entire CNVM appeared more effective than partial photocoagulatin of the CNVM.", "contents": "System of notation for photocoagulation in the presumed ocular histoplasmosis syndrome. Using a notation system for evaluation of photocoagulatin of the choroidal neovascular membrane (CNVM) in presumed ocular histoplasmosis syndrome (POHS) demonstrated several advantages, which are: (1) a projector system which allowed excellent magnification of the CNVM and its distance from the foveal avascular zone (FAZ); (2) direct study of fluorescein angiographic negatives; (3) good comparison of pretreatment of posttreatment lesions in relation to FAZ; (4) a precise measurement of the surface area of each CNVM and its distance from the FAZ; and (5) an accurate and rapid way of recording and comparing data. This notation system appears valuable as a means of standardizing the examination and photographic findings from multiple institutions. Results of photocoagulatin of the CNVM are presented. Photocoagulatin of the entire CNVM appeared more effective than partial photocoagulatin of the CNVM."}
{"id": "PMID:464511", "title": "[Surgery for laryngeal stenosis in infants in liable in case of failure to produce a worse condition resulting on a definitive disability (author's transl)].", "content": "The proper treatment for these stenosis is the \"evolutive calibrage\" which takes advantage of the normal growth of the larynx with no risk of aggravation.", "contents": "[Surgery for laryngeal stenosis in infants in liable in case of failure to produce a worse condition resulting on a definitive disability (author's transl)]. The proper treatment for these stenosis is the \"evolutive calibrage\" which takes advantage of the normal growth of the larynx with no risk of aggravation."}
{"id": "PMID:464512", "title": "[Management of sialorrhea in mentally retarded children by salivary denervation (6 cases) (author's transl)].", "content": "Sialorrhea in mentally handicapped children makes rehabilitation difficult and may provoke dyspneic spells. We have interrupted in 6 cases the parasympathetic innervation to the major salivary glands by a bilateral intratympanic neurectomy of Jacobson's nerve and chorda tympani. Results were satisfactory in 2/3 of cases.", "contents": "[Management of sialorrhea in mentally retarded children by salivary denervation (6 cases) (author's transl)]. Sialorrhea in mentally handicapped children makes rehabilitation difficult and may provoke dyspneic spells. We have interrupted in 6 cases the parasympathetic innervation to the major salivary glands by a bilateral intratympanic neurectomy of Jacobson's nerve and chorda tympani. Results were satisfactory in 2/3 of cases."}
{"id": "PMID:464514", "title": "[Cochlear mechanics: new experimental data (author's transl)].", "content": "The goal of this research is to confirm or disprove the presently accepted theories concerning the cochlear mechanism using: -- enlarged mechanical models of the cochlea, reproducing the known anatomy as accurately as possible, -- true sinusoidal stimuli or transient, -- objective documents (photographs and films).", "contents": "[Cochlear mechanics: new experimental data (author's transl)]. The goal of this research is to confirm or disprove the presently accepted theories concerning the cochlear mechanism using: -- enlarged mechanical models of the cochlea, reproducing the known anatomy as accurately as possible, -- true sinusoidal stimuli or transient, -- objective documents (photographs and films)."}
{"id": "PMID:464513", "title": "[The origin of acoustic neurionoma. An ultrastructural study of operated neurinoma incipiens (author's transl)].", "content": "The origin of eighth nerve neurinoma has been studied by several authors with the light microscope, particularly on serial sections of decalcified temporal bone. The microsurgical approach of small neurinomas has led the present authors to an ultrastructural study of this problem. In the cases in which the origin could be seen, the tumor appeared to arise from the ganglion vestibulare (Scarpae). This fact is correlated with the neural crest origin of the ganglion, and with its ultrastructural characteristics in the normal human.", "contents": "[The origin of acoustic neurionoma. An ultrastructural study of operated neurinoma incipiens (author's transl)]. The origin of eighth nerve neurinoma has been studied by several authors with the light microscope, particularly on serial sections of decalcified temporal bone. The microsurgical approach of small neurinomas has led the present authors to an ultrastructural study of this problem. In the cases in which the origin could be seen, the tumor appeared to arise from the ganglion vestibulare (Scarpae). This fact is correlated with the neural crest origin of the ganglion, and with its ultrastructural characteristics in the normal human."}
{"id": "PMID:464515", "title": "[The treatment of sub-glottic stenosis by continuous dilatation. Long term results (author's transl)].", "content": "From 1973 to 1977, 15 patients with laryngeal stenosis were treated by a method of continuous dilatation in the ENT department of the \"Enfants Malades\" Hospital. The results were viewed with regard to the importance of the complications, the chance of decanulation, the carrying time of the canula, adaptation to effort, functional respiratory tests, the value of language, intellectual and psychic repercussions, and repercussions on the social life. In this series the method of dilatation seems remarkably reliable.", "contents": "[The treatment of sub-glottic stenosis by continuous dilatation. Long term results (author's transl)]. From 1973 to 1977, 15 patients with laryngeal stenosis were treated by a method of continuous dilatation in the ENT department of the \"Enfants Malades\" Hospital. The results were viewed with regard to the importance of the complications, the chance of decanulation, the carrying time of the canula, adaptation to effort, functional respiratory tests, the value of language, intellectual and psychic repercussions, and repercussions on the social life. In this series the method of dilatation seems remarkably reliable."}
{"id": "PMID:464516", "title": "[Effects of neurectomy of the tympanic plexus on secretion of the parotid gland in man. Histopathological, histochemical and clinical study (author's transl)].", "content": "This study involved five patients suffering from bilateral chronic parotiditis and then with a persistent unilateral fistula of the parotid gland. Two types of parotid biopsy were examined: the first taken during surgery and the second six months later. All of the specimens underwent histopathological and histochemical investigations. Definitive degeneration and atrophic type changes were seen in all the parotid fragments removed six months after selective neurectomy of the tympanic plexus. Six months after surgery, all of the patients were cured.", "contents": "[Effects of neurectomy of the tympanic plexus on secretion of the parotid gland in man. Histopathological, histochemical and clinical study (author's transl)]. This study involved five patients suffering from bilateral chronic parotiditis and then with a persistent unilateral fistula of the parotid gland. Two types of parotid biopsy were examined: the first taken during surgery and the second six months later. All of the specimens underwent histopathological and histochemical investigations. Definitive degeneration and atrophic type changes were seen in all the parotid fragments removed six months after selective neurectomy of the tympanic plexus. Six months after surgery, all of the patients were cured."}
{"id": "PMID:464517", "title": "[Immediate improvement in facial paralysis after surgical decompression (author's transl)].", "content": "17 cases of severe facial paralysis were studied before and after microsurgical decompression, by quantitative electromyography (electromyogram integrated in volts) at the site of the zyogmatic arch. -- In 76% of cases, no significant improvement was measured. -- An immediate, frank improvement occurred infrequently (12%), was short-lived and did not have any influence on the final result.", "contents": "[Immediate improvement in facial paralysis after surgical decompression (author's transl)]. 17 cases of severe facial paralysis were studied before and after microsurgical decompression, by quantitative electromyography (electromyogram integrated in volts) at the site of the zyogmatic arch. -- In 76% of cases, no significant improvement was measured. -- An immediate, frank improvement occurred infrequently (12%), was short-lived and did not have any influence on the final result."}
{"id": "PMID:464518", "title": "[Auricular tuberculosis with regressive facial palsy after surgical intervention (author's transl)].", "content": "The authors have observed the complete regression of a facial palsy in a tuberculous otitis by precocious decompression of the nerve and anti-tuberculous medical treatment.", "contents": "[Auricular tuberculosis with regressive facial palsy after surgical intervention (author's transl)]. The authors have observed the complete regression of a facial palsy in a tuberculous otitis by precocious decompression of the nerve and anti-tuberculous medical treatment."}
{"id": "PMID:464519", "title": "[Medullary carcinoma of the thyroid (author's transl)].", "content": "Medullary carcinoma of the thyroid develops in the parafollicular C cells, the origin of which is in the neural crest. It is characterized clinically by the presence of motor diarrhea, vasomotor attacks, and immunologically by the presence of an amyloid stroma and the appearance of an anaplastic carcinoma. It is frequently associated with a pheochromocytoma (Sipple's syndrome), parathyroid adenomas and cytaneo mucosal manifestations (phacomatosis, multiple mucosal neuromas). The C cells may secrete thyrocalcitonin and the estimation of the substance may be sometimes used in pre-operative diagnosis and, above all, subsequent observation and examination for the presence of metastases. Treatment is based upon surgery (total thyroidectomy) with lymphnode dissection. Complementary treatment (radiotherapy, chemotherapy, opotherapy) is given only when surgery is inedequate or recurrences occur.", "contents": "[Medullary carcinoma of the thyroid (author's transl)]. Medullary carcinoma of the thyroid develops in the parafollicular C cells, the origin of which is in the neural crest. It is characterized clinically by the presence of motor diarrhea, vasomotor attacks, and immunologically by the presence of an amyloid stroma and the appearance of an anaplastic carcinoma. It is frequently associated with a pheochromocytoma (Sipple's syndrome), parathyroid adenomas and cytaneo mucosal manifestations (phacomatosis, multiple mucosal neuromas). The C cells may secrete thyrocalcitonin and the estimation of the substance may be sometimes used in pre-operative diagnosis and, above all, subsequent observation and examination for the presence of metastases. Treatment is based upon surgery (total thyroidectomy) with lymphnode dissection. Complementary treatment (radiotherapy, chemotherapy, opotherapy) is given only when surgery is inedequate or recurrences occur."}
{"id": "PMID:464520", "title": "[Surgery of the facial nerve (author's transl)].", "content": "The indication of facial nerve surgery are wider than currently believed. The incision of the fibrous sheath, the suture termino-terminal, the nervous graft even very long, the hypoglosso anastomosis give all better results than all other surgical technics. The potentiality of nervous regeneration exist in old people and after very long delay.", "contents": "[Surgery of the facial nerve (author's transl)]. The indication of facial nerve surgery are wider than currently believed. The incision of the fibrous sheath, the suture termino-terminal, the nervous graft even very long, the hypoglosso anastomosis give all better results than all other surgical technics. The potentiality of nervous regeneration exist in old people and after very long delay."}
{"id": "PMID:464521", "title": "Cellular immunity in patients with laryngeal cancer developing additional primary malignant tumors.", "content": "Eleven patients with laryngeal cancer associated with additional primary tumors underwent cutaneous delayed hypersensitivity tests and lymphocyte stimulation studies with phytohemagglutinin for evaluation of their cellular immunity. Five of the 11 patients were anergic or hypoergic as shown by skin tests to recall antigens and seven of nine patients had impaired lymphocyte transformation. Depressed immunity was more often observed in patients with an active malignancy. The level of serum immunoglobulins was elevated in two of eight studied patients, and in the remaining patients was within the normal range. All patients had a history of excessive smoking. Most frequently, second tumors were found in the lung, urinary tract and skin. The prognosis seemed to be determined by the additional tumor rather than by the laryngeal cancer.", "contents": "Cellular immunity in patients with laryngeal cancer developing additional primary malignant tumors. Eleven patients with laryngeal cancer associated with additional primary tumors underwent cutaneous delayed hypersensitivity tests and lymphocyte stimulation studies with phytohemagglutinin for evaluation of their cellular immunity. Five of the 11 patients were anergic or hypoergic as shown by skin tests to recall antigens and seven of nine patients had impaired lymphocyte transformation. Depressed immunity was more often observed in patients with an active malignancy. The level of serum immunoglobulins was elevated in two of eight studied patients, and in the remaining patients was within the normal range. All patients had a history of excessive smoking. Most frequently, second tumors were found in the lung, urinary tract and skin. The prognosis seemed to be determined by the additional tumor rather than by the laryngeal cancer."}
{"id": "PMID:464523", "title": "Juvenile fibromatosis.", "content": "Juvenile fibromatosis is a benign lesion with locally aggressive characteristics. Wide surgical excision is the treatment of choice. Radiation therapy can be used for inoperable cases or as adjunctive therapy. Steroid therapy has not been used extensively and deserves further evaluation. In the head and neck area, juvenile fibromatosis must be very carefully diagnosed and treated due to the serious consequences that result from inadequate control.", "contents": "Juvenile fibromatosis. Juvenile fibromatosis is a benign lesion with locally aggressive characteristics. Wide surgical excision is the treatment of choice. Radiation therapy can be used for inoperable cases or as adjunctive therapy. Steroid therapy has not been used extensively and deserves further evaluation. In the head and neck area, juvenile fibromatosis must be very carefully diagnosed and treated due to the serious consequences that result from inadequate control."}
{"id": "PMID:464522", "title": "Width of the internal auditory canal. A histological study.", "content": "Vertical and horizontal measurements were made of the widths of the internal auditory canals of 435 histologically prepared temporal bones. The mean vertical width, measured at the porus acusticus, was 3.68 mm (range 2.10 to 5.26 mm), and the mean horizontal width, measured in the middle region of the canal, was 3.72 mm (range 2 to 5.8 mm). The 144 ears selected for study because they exhibited slowly progressive sensorineural hearing loss showed a distribution of canal widths similar to that of the group as a whole. Histological examination of the internal auditory canals failed to show any type of soft tissue lesion which might have contributed to narrowing of the canals. The study provides no anatomical or histological evidence for the existence of a small internal auditory canal syndrome.", "contents": "Width of the internal auditory canal. A histological study. Vertical and horizontal measurements were made of the widths of the internal auditory canals of 435 histologically prepared temporal bones. The mean vertical width, measured at the porus acusticus, was 3.68 mm (range 2.10 to 5.26 mm), and the mean horizontal width, measured in the middle region of the canal, was 3.72 mm (range 2 to 5.8 mm). The 144 ears selected for study because they exhibited slowly progressive sensorineural hearing loss showed a distribution of canal widths similar to that of the group as a whole. Histological examination of the internal auditory canals failed to show any type of soft tissue lesion which might have contributed to narrowing of the canals. The study provides no anatomical or histological evidence for the existence of a small internal auditory canal syndrome."}
{"id": "PMID:464524", "title": "Nasal hemangioma of pregnancy.", "content": "The nasal hemangioma of pregnancy is an uncommon but impressive cause of epistaxis. One patient with this condition recently treated at the Keller Army Hospital, West Point, exemplifies the problems encountered in the diagnosis and treatment of this puzzling disorder.", "contents": "Nasal hemangioma of pregnancy. The nasal hemangioma of pregnancy is an uncommon but impressive cause of epistaxis. One patient with this condition recently treated at the Keller Army Hospital, West Point, exemplifies the problems encountered in the diagnosis and treatment of this puzzling disorder."}
{"id": "PMID:464526", "title": "Necrotizing sialometaplasia of the nasopharynx.", "content": "Necrotizing sialometaplasia is an uncommon salivary gland disease originally described by Abrams et al in 1973. The disease may occur wherever salivary gland tissue is found. Theories on the etiology of this disorder have been advanced, but never definitely determined. Treatment consists of adequate biopsy and observation until healing occurs at six to eight weeks. While the disease is considered benign, its similarity to more aggressive neoplasms can be both disturbing and misleading to the patient and the clinician. Such a case of necrotizing sialometaplasia of the nasopharynx is presented to demonstrate the clinical and histological similarity of this disease to carcinoma. In this case, the patient first presented with a neck mass which could easily have been mistaken for a regional metastasis. The current literature is reviewed.", "contents": "Necrotizing sialometaplasia of the nasopharynx. Necrotizing sialometaplasia is an uncommon salivary gland disease originally described by Abrams et al in 1973. The disease may occur wherever salivary gland tissue is found. Theories on the etiology of this disorder have been advanced, but never definitely determined. Treatment consists of adequate biopsy and observation until healing occurs at six to eight weeks. While the disease is considered benign, its similarity to more aggressive neoplasms can be both disturbing and misleading to the patient and the clinician. Such a case of necrotizing sialometaplasia of the nasopharynx is presented to demonstrate the clinical and histological similarity of this disease to carcinoma. In this case, the patient first presented with a neck mass which could easily have been mistaken for a regional metastasis. The current literature is reviewed."}
{"id": "PMID:464527", "title": "Cochlear and brain stem responses in hearing loss following neonatal hyperbilirubinemia.", "content": "The site of lesion in hearing loss following neonatal hyperbilirubinemia is unclear. Histopathological studies have implicated the brain stem auditory nuclei while other investigations have hinted at a lesion in the cochlea. In order to clarify this issue, attempts were made to record responses from the auditory pathway in 13 patients with hearing loss following neonatal hyperbilirubinemia. The neural response from the auditory nerve was absent in 11 of the 13 patients and present only in response to high intensity stimuli in 2 patients. However, the response of the cochlear hair cells (cochlear microphonic potential) was present in 9 of the 13 patients. In most other cases of sensorineural hearing loss, with no history of hyperbilirubinemia, the hair cell response was absent. This is functional evidence for auditory nerve damage in cases of hearing loss following neonatal hyperbilirubinemia while the hair cells are spared.", "contents": "Cochlear and brain stem responses in hearing loss following neonatal hyperbilirubinemia. The site of lesion in hearing loss following neonatal hyperbilirubinemia is unclear. Histopathological studies have implicated the brain stem auditory nuclei while other investigations have hinted at a lesion in the cochlea. In order to clarify this issue, attempts were made to record responses from the auditory pathway in 13 patients with hearing loss following neonatal hyperbilirubinemia. The neural response from the auditory nerve was absent in 11 of the 13 patients and present only in response to high intensity stimuli in 2 patients. However, the response of the cochlear hair cells (cochlear microphonic potential) was present in 9 of the 13 patients. In most other cases of sensorineural hearing loss, with no history of hyperbilirubinemia, the hair cell response was absent. This is functional evidence for auditory nerve damage in cases of hearing loss following neonatal hyperbilirubinemia while the hair cells are spared."}
{"id": "PMID:464528", "title": "Congenital cerebrospinal otorrhea.", "content": "Four locations for congenital cerebrospinal fluid fistula in the region of a normal labyrinth are reviewed. A congenital leak may occur through the petromastoid canal, a wide cochlear aqueduct, Hyrtl's fissure, or the facial canal. A fistula through the initial segment of the fallopian canal was successfully repaired in a two-year-old boy who had three episodes of meningitis following otitis media. Knowledge of these four sites of congenital defects provides a guideline for the surgeon in the identification and repair of cerebrospinal fluid leaks in the region of the labyrinth.", "contents": "Congenital cerebrospinal otorrhea. Four locations for congenital cerebrospinal fluid fistula in the region of a normal labyrinth are reviewed. A congenital leak may occur through the petromastoid canal, a wide cochlear aqueduct, Hyrtl's fissure, or the facial canal. A fistula through the initial segment of the fallopian canal was successfully repaired in a two-year-old boy who had three episodes of meningitis following otitis media. Knowledge of these four sites of congenital defects provides a guideline for the surgeon in the identification and repair of cerebrospinal fluid leaks in the region of the labyrinth."}
{"id": "PMID:464529", "title": "Fibrinolytic activity in patients with acute tonsillitis.", "content": "Three parameters in blood fibrinolysis, viz, the levels of fibrinogen, plasminogen, and fibrinogen and/or fibrin degradation products (FDP), were measured in patients with acute tonsillitis and the results were compared with those for healthy adults. The fibrinogen content in acute tonsillitis increased significantly (P less than .001), that of plasminogen decreased significantly (P less than .005), while FDP showed a higher value (P less than .01). The significance of the fibrinolytic system in acute tonsillitis is discussed.", "contents": "Fibrinolytic activity in patients with acute tonsillitis. Three parameters in blood fibrinolysis, viz, the levels of fibrinogen, plasminogen, and fibrinogen and/or fibrin degradation products (FDP), were measured in patients with acute tonsillitis and the results were compared with those for healthy adults. The fibrinogen content in acute tonsillitis increased significantly (P less than .001), that of plasminogen decreased significantly (P less than .005), while FDP showed a higher value (P less than .01). The significance of the fibrinolytic system in acute tonsillitis is discussed."}
{"id": "PMID:464530", "title": "Anatomical effects of sudden middle ear pressure changes.", "content": "Acute middle ear (ME) and inner ear changes following brief unilateral phasic ME pressure changes (up to +/- 6000/mm H2O) were studied in the guinea pig. Middle ear findings included perforation of the tympanic membrane, serous and serosanguinous exudate and hemorrhage of tympanic membrane and periosteal vessels. Changes were related to magnitude of applied pressure. Perforation and hemorrhage were more commonly seen with negative rather than positive pressure. Air bubbles behind the round window were seen with positive pressures. Occasional distortion, but never perforation of the round window, was noted. Hemorrhage of the scala tympani was observed with both positive and negative pressures; scala vestibuli hemorrhage was found with negative ME pressure. In some instances pressure direction and magnitude related changes were seen in the contralateral ear.", "contents": "Anatomical effects of sudden middle ear pressure changes. Acute middle ear (ME) and inner ear changes following brief unilateral phasic ME pressure changes (up to +/- 6000/mm H2O) were studied in the guinea pig. Middle ear findings included perforation of the tympanic membrane, serous and serosanguinous exudate and hemorrhage of tympanic membrane and periosteal vessels. Changes were related to magnitude of applied pressure. Perforation and hemorrhage were more commonly seen with negative rather than positive pressure. Air bubbles behind the round window were seen with positive pressures. Occasional distortion, but never perforation of the round window, was noted. Hemorrhage of the scala tympani was observed with both positive and negative pressures; scala vestibuli hemorrhage was found with negative ME pressure. In some instances pressure direction and magnitude related changes were seen in the contralateral ear."}
{"id": "PMID:464531", "title": "Median palatal cyst. A reminder of palate fusion.", "content": "The existence of a median palatal cyst has been questioned. Such a lesion would represent an unusual anomaly of a unique embryological process. The radiologic and pathologic criteria necessary to establish such a diagnosis are inconclusive in the ten case reports that have appeared in the English language literature. A median palatal cyst that is distinct from other palatal defects would have specific characteristics that included: 1) a true epithelial-lined cyst; 2) no salivary gland, vascular, or neural elements in the cyst wall; and 3) location in the palate at a distance sufficiently posterior to avoid confusion with structures of the nasal palatine region. We report the findings of a palate lesion excised from a 27-year-old male which by location and histology were consistent only with a median palatal cyst. These data appear to authenticate, for the first time, the median palatal cyst as a distinct pathological entity.", "contents": "Median palatal cyst. A reminder of palate fusion. The existence of a median palatal cyst has been questioned. Such a lesion would represent an unusual anomaly of a unique embryological process. The radiologic and pathologic criteria necessary to establish such a diagnosis are inconclusive in the ten case reports that have appeared in the English language literature. A median palatal cyst that is distinct from other palatal defects would have specific characteristics that included: 1) a true epithelial-lined cyst; 2) no salivary gland, vascular, or neural elements in the cyst wall; and 3) location in the palate at a distance sufficiently posterior to avoid confusion with structures of the nasal palatine region. We report the findings of a palate lesion excised from a 27-year-old male which by location and histology were consistent only with a median palatal cyst. These data appear to authenticate, for the first time, the median palatal cyst as a distinct pathological entity."}
{"id": "PMID:464532", "title": "Surgical anatomy of the orbit.", "content": "When operating in and around the orbit, the key to a successful operative result is precise, anatomic localization. This study was constructed to give pertinent anatomic measurements to which the maxillofacial surgeon may refer. The safe distances noted from this study are: 1) medially 30 mm from the anterior lacrimal crest; 2) inferiorly 25 mm from the infraorbital foramen; 3) superiorly 30 mm from the supraorbital notch; and 4) laterally 25 mm from the frontozygomatic suture.", "contents": "Surgical anatomy of the orbit. When operating in and around the orbit, the key to a successful operative result is precise, anatomic localization. This study was constructed to give pertinent anatomic measurements to which the maxillofacial surgeon may refer. The safe distances noted from this study are: 1) medially 30 mm from the anterior lacrimal crest; 2) inferiorly 25 mm from the infraorbital foramen; 3) superiorly 30 mm from the supraorbital notch; and 4) laterally 25 mm from the frontozygomatic suture."}
{"id": "PMID:464537", "title": "A simple micromethod for 25-hydroxyvitamin D estimation.", "content": "A quick and simple method for estimating 25-hydroxyvitamin D is described, It involves dichloromethane extraction followed by competitive protein-binding assay without chromatography. This assay can be performed on 100 microliter of serum and enables 50 samples to be estimated in one working day. The simplicity, speed, and sample size of this method make it very suitable for use in a routine clinical biochemistry laboratory. It is particularly useful when calcium disorders secondary to abnormalities in vitamin D intake and metabolism are suspected.", "contents": "A simple micromethod for 25-hydroxyvitamin D estimation. A quick and simple method for estimating 25-hydroxyvitamin D is described, It involves dichloromethane extraction followed by competitive protein-binding assay without chromatography. This assay can be performed on 100 microliter of serum and enables 50 samples to be estimated in one working day. The simplicity, speed, and sample size of this method make it very suitable for use in a routine clinical biochemistry laboratory. It is particularly useful when calcium disorders secondary to abnormalities in vitamin D intake and metabolism are suspected."}
{"id": "PMID:464538", "title": "Screening for Cushing's syndrome using early morning urine samples.", "content": "Urinary free 11-hydroxycorticosteroid/creatinine ratios (UFC/Cr)were determined in early morning urine samples from 138 obese subjects attending endocrine clinics in the Glasgow area. The majority of patients (128) had UFC/Cr ratios within the normal range for non-obese subjects (5-55 mumol/mol). Of those with elevated UFC/Cr ratios, further investigations of the hypothalamic-pituitary-adrenal axis confirmed a diagnosis of Cushing's syndrome due to bilateral adrenal hyperplasia in six of the cases and adrenal tumours in a further two patients. In the remaining two patients there was evidence of intermittent adrenal hyperactivity with inconsistent responses to insulin-induced hypoglycaemia tests.", "contents": "Screening for Cushing's syndrome using early morning urine samples. Urinary free 11-hydroxycorticosteroid/creatinine ratios (UFC/Cr)were determined in early morning urine samples from 138 obese subjects attending endocrine clinics in the Glasgow area. The majority of patients (128) had UFC/Cr ratios within the normal range for non-obese subjects (5-55 mumol/mol). Of those with elevated UFC/Cr ratios, further investigations of the hypothalamic-pituitary-adrenal axis confirmed a diagnosis of Cushing's syndrome due to bilateral adrenal hyperplasia in six of the cases and adrenal tumours in a further two patients. In the remaining two patients there was evidence of intermittent adrenal hyperactivity with inconsistent responses to insulin-induced hypoglycaemia tests."}
{"id": "PMID:464539", "title": "Re-examination of effect of paracetamol on serum uric acid measured by phosphotungstic acid reduction.", "content": "The drug paracetamol (N-acetyl-p-aminophenol; acetaminophen) caused a spurious increase in serum uric acid measured by phosphotungstic acid reduction methods. However, the increase was less than 0.12 mmol/1 at plasma levels of paracetamol found in overdosage (40 mg/100 ml) and was small at therapeutic concentrations (less than 4 mg/100 ml). It is concluded that few patients with joint pain who have taken paracetamol paracetamol as an analgesic will have clinically misleading values for serum uric acid.", "contents": "Re-examination of effect of paracetamol on serum uric acid measured by phosphotungstic acid reduction. The drug paracetamol (N-acetyl-p-aminophenol; acetaminophen) caused a spurious increase in serum uric acid measured by phosphotungstic acid reduction methods. However, the increase was less than 0.12 mmol/1 at plasma levels of paracetamol found in overdosage (40 mg/100 ml) and was small at therapeutic concentrations (less than 4 mg/100 ml). It is concluded that few patients with joint pain who have taken paracetamol paracetamol as an analgesic will have clinically misleading values for serum uric acid."}
{"id": "PMID:464534", "title": "Extracranial meningioma.", "content": "A patient is presented with findings of separate intracranial and extracranial meningiomas, each of a different histologic type. A calcified fibrous meningioma, with secondary psammomatous features, presented as a left neck mass associated with hoarseness, dysphagia, a unilateral facial weakness and hearing loss. A noncalcified asymptomatic intracranial syncytial meningioma was discovered in the left frontal lobe after computerized tomographic and angiographic study of the cranial contents. The origin of the extracranial meningioma producing multiple unilateralcranial nerve disturbances and serous otitis media is discussed. The noncontiguous tumors in this patients are felt to have separate origins, with the extracranial lesion most likely arising in the temporal bone.", "contents": "Extracranial meningioma. A patient is presented with findings of separate intracranial and extracranial meningiomas, each of a different histologic type. A calcified fibrous meningioma, with secondary psammomatous features, presented as a left neck mass associated with hoarseness, dysphagia, a unilateral facial weakness and hearing loss. A noncalcified asymptomatic intracranial syncytial meningioma was discovered in the left frontal lobe after computerized tomographic and angiographic study of the cranial contents. The origin of the extracranial meningioma producing multiple unilateralcranial nerve disturbances and serous otitis media is discussed. The noncontiguous tumors in this patients are felt to have separate origins, with the extracranial lesion most likely arising in the temporal bone."}
{"id": "PMID:464535", "title": "Inhibition of labyrinthine nystagmus by visual fixation: effects of ablation of visual cortex and superior colliculi.", "content": "A study was conducted to destroy two specific areas of the cat's visual system in order to determine if these lesions would affect the visual inhibition of calorically-induced vestibular nystagmus. The occipital visual cortex was removed in eight cats and the superior colliculi were removed bilaterally in nine cats. Postoperative vestibular testing revealed no significant change in the electronystagmography tracings and response to visual fixation. These findings suggest that, in cats, the visual inhibition of labyrinthine nystagmus is not dependent upon the integrity of the visual cortex or superior colliculi. The hypothesis is brought forward that the visual inhibition of the vestibular nystagmus is merely a reflex of the brain stem to light stimulus, mediated via the cerebellum.", "contents": "Inhibition of labyrinthine nystagmus by visual fixation: effects of ablation of visual cortex and superior colliculi. A study was conducted to destroy two specific areas of the cat's visual system in order to determine if these lesions would affect the visual inhibition of calorically-induced vestibular nystagmus. The occipital visual cortex was removed in eight cats and the superior colliculi were removed bilaterally in nine cats. Postoperative vestibular testing revealed no significant change in the electronystagmography tracings and response to visual fixation. These findings suggest that, in cats, the visual inhibition of labyrinthine nystagmus is not dependent upon the integrity of the visual cortex or superior colliculi. The hypothesis is brought forward that the visual inhibition of the vestibular nystagmus is merely a reflex of the brain stem to light stimulus, mediated via the cerebellum."}
{"id": "PMID:464540", "title": "Classification of tumors of the ovary: developmental and ultrastructural considerations.", "content": "The classification of ovarian tumors presents a difficult problem because of the great variety of tumor types that can occur. The complex structure of the normal ovary and the diversity of cell types present at different stages of development contribute to this difficulty. Developmental and ultrastructural studies have helped to clarify the problem by indicating specific cell types that correspond directly with the major tumor categories. Tumors may thus be grouped as being of epithelial, germ cell or sex cord stromal origin. The ultrastructural features of tumors in the different categories indicate common characteristics shared with corresponding cell types in the developing ovary. The findings clearly support a histogenetic approach in the classification of ovarian tumors.", "contents": "Classification of tumors of the ovary: developmental and ultrastructural considerations. The classification of ovarian tumors presents a difficult problem because of the great variety of tumor types that can occur. The complex structure of the normal ovary and the diversity of cell types present at different stages of development contribute to this difficulty. Developmental and ultrastructural studies have helped to clarify the problem by indicating specific cell types that correspond directly with the major tumor categories. Tumors may thus be grouped as being of epithelial, germ cell or sex cord stromal origin. The ultrastructural features of tumors in the different categories indicate common characteristics shared with corresponding cell types in the developing ovary. The findings clearly support a histogenetic approach in the classification of ovarian tumors."}
{"id": "PMID:464541", "title": "Measurement of thyroglobulin in the circulation: clinical and technical considerations.", "content": "The concentration of thyroglobulin (TG) in the circulation can be measured by a sensitive, specific, convenient radioimmunoassay. TG is found in the circulation of virtually all normal subjects. It is present in elevated concentrations in patients with a wide variety of thyroid diseases including benign and malignant tumors, multinodular goiter, subacute thyroiditis, Graves' disease and others. At the present time, the most important clinical role of TG measurements is in the evaluation of patients who have been treated for thyroid cancer. As greater clinical correlation is obtained, the usefulness of TG determinations will increase. Anti-TG autoantibodies cause false results in the assay and present the major technical problem which needs to be resolved.", "contents": "Measurement of thyroglobulin in the circulation: clinical and technical considerations. The concentration of thyroglobulin (TG) in the circulation can be measured by a sensitive, specific, convenient radioimmunoassay. TG is found in the circulation of virtually all normal subjects. It is present in elevated concentrations in patients with a wide variety of thyroid diseases including benign and malignant tumors, multinodular goiter, subacute thyroiditis, Graves' disease and others. At the present time, the most important clinical role of TG measurements is in the evaluation of patients who have been treated for thyroid cancer. As greater clinical correlation is obtained, the usefulness of TG determinations will increase. Anti-TG autoantibodies cause false results in the assay and present the major technical problem which needs to be resolved."}
{"id": "PMID:464547", "title": "Voluntary motor commands in human ballistic movements.", "content": "The integrated electromyogram of the first dorsal interosseous muscle in normal humans presents a brief early burst in voluntary ballistic abduction of the index finger. Unexpected decrease in torque load does not change the burst. By contrast, in fast ramp abduction with a time-to-peak of 0.5 second, the electromyographic activity is fairly continuous up to the peak displacement, and it is silenced with a delay of 50 msec by unexpected unloading. Thus fast ramp, but not ballistic, voluntary contractions are under current long loop control from proprioceptive input. In a second set of experiments, the analysis of single motor unit potentials with highly selective electrodes in interosseous muscle indicated that ballistic thresholds are smaller than ramp thresholds under either isotonic or isometric conditions. However, the recruitment rank of the motor units was identical in finger abductions carried out in either ramp or ballistic modes.", "contents": "Voluntary motor commands in human ballistic movements. The integrated electromyogram of the first dorsal interosseous muscle in normal humans presents a brief early burst in voluntary ballistic abduction of the index finger. Unexpected decrease in torque load does not change the burst. By contrast, in fast ramp abduction with a time-to-peak of 0.5 second, the electromyographic activity is fairly continuous up to the peak displacement, and it is silenced with a delay of 50 msec by unexpected unloading. Thus fast ramp, but not ballistic, voluntary contractions are under current long loop control from proprioceptive input. In a second set of experiments, the analysis of single motor unit potentials with highly selective electrodes in interosseous muscle indicated that ballistic thresholds are smaller than ramp thresholds under either isotonic or isometric conditions. However, the recruitment rank of the motor units was identical in finger abductions carried out in either ramp or ballistic modes."}
{"id": "PMID:464542", "title": "Carcinogen activation by human uterine enzymes.", "content": "Three topics are briefly reviewed relating to carcinogenesis of estrogen responsive tissues: (a) enzymology of benzo(a)pyrene activation by human tissues, (b) microsomal activation of estrogens to estrogen arene oxides and (c) estrogen and progesterone receptor studies in endometrial carcinoma. The following working hypothesis is stated on the etiology of gynecologic tumors: \"Environmental chemicals, such as cigarette smoke, polycyclic and polyhalogenated hydrocarbons, etc., induce special forms of cytochrome P-450 monooxygenase and related enzyme systems which can activate endogenous or prescribed estrogens and non-steroid antiestrogens to act as initiators and/or promoters of neoplasia in estrogen-dependent organs.\" The role of estrogen receptors is perceived as a homing device or cellular \"Trojan Horse\" for these activated estrogens.", "contents": "Carcinogen activation by human uterine enzymes. Three topics are briefly reviewed relating to carcinogenesis of estrogen responsive tissues: (a) enzymology of benzo(a)pyrene activation by human tissues, (b) microsomal activation of estrogens to estrogen arene oxides and (c) estrogen and progesterone receptor studies in endometrial carcinoma. The following working hypothesis is stated on the etiology of gynecologic tumors: \"Environmental chemicals, such as cigarette smoke, polycyclic and polyhalogenated hydrocarbons, etc., induce special forms of cytochrome P-450 monooxygenase and related enzyme systems which can activate endogenous or prescribed estrogens and non-steroid antiestrogens to act as initiators and/or promoters of neoplasia in estrogen-dependent organs.\" The role of estrogen receptors is perceived as a homing device or cellular \"Trojan Horse\" for these activated estrogens."}
{"id": "PMID:464548", "title": "Single-dose pharmacokinetics and anticonvulsant efficacy of primidone in mice.", "content": "The pharmacokinetics and efficacy of the anticonvulsant primidone (PRM) and its active metabolites, phenobarbital (PB) and phenylethylmalonamide (PEMA), were studied after single-dose administration in mice. The half-life of PB is twice that of PRM and PEMA. The plasma/brain ratios provide evidence of poor penetration of PRM into brain. The results support our findings of negligible or absent PRM concentrations in the brains of patients on primidone therapy who were undergoing surgery for intractable epilepsy. The anticonvulsant properties of PRM, PB, and PEMA against maximal electroshock in mice were also studied with the use of the metabolic inhibitor SKF 525A. The half-life, potency, peak anticonvulsant effect, and effective dose curves of these compounds indicate that the anticonvulsant effect of short-term oral PRM administration in mice is from derived PB.", "contents": "Single-dose pharmacokinetics and anticonvulsant efficacy of primidone in mice. The pharmacokinetics and efficacy of the anticonvulsant primidone (PRM) and its active metabolites, phenobarbital (PB) and phenylethylmalonamide (PEMA), were studied after single-dose administration in mice. The half-life of PB is twice that of PRM and PEMA. The plasma/brain ratios provide evidence of poor penetration of PRM into brain. The results support our findings of negligible or absent PRM concentrations in the brains of patients on primidone therapy who were undergoing surgery for intractable epilepsy. The anticonvulsant properties of PRM, PB, and PEMA against maximal electroshock in mice were also studied with the use of the metabolic inhibitor SKF 525A. The half-life, potency, peak anticonvulsant effect, and effective dose curves of these compounds indicate that the anticonvulsant effect of short-term oral PRM administration in mice is from derived PB."}
{"id": "PMID:464544", "title": "Performance evaluation of a blood gas system in a central and satellite laboratory.", "content": "In many hospitals blood gases are performed in several laboratories. It is important to establish that values obtained in all laboratories are identical and that the same quality control program is used. Evaluation of the performance of blood gas apparatus has been made in two of our laboratories. Result reproducibility was assessed by means of gas analyzer system control. Measurements were made in duplicate of patients' specimens in the same instrument and duplicate measurements of patient samples with both instruments. A13 day evaluation using this system was carried out in both laboratories during all shifts and showed excellent agreement with the manufacturer's reported values. The combined standard deviation for pH at all levels was 0.009,pCO2 -0.83 mmHg and pO2 - 3.9 mmHg. Patients' samples analyzed in the same instrument showed an absolute average difference between duplicates for pH of 0.003, pCO2 - 0.62 mmHg and pO2 -0.87 mmHg. The comparison of duplicate samples in the two instruments in the different laboratories showed an absolute average difference for pH of 0.009,pCO2-1.7mmHg and pO2 - 1.6 mmHg.", "contents": "Performance evaluation of a blood gas system in a central and satellite laboratory. In many hospitals blood gases are performed in several laboratories. It is important to establish that values obtained in all laboratories are identical and that the same quality control program is used. Evaluation of the performance of blood gas apparatus has been made in two of our laboratories. Result reproducibility was assessed by means of gas analyzer system control. Measurements were made in duplicate of patients' specimens in the same instrument and duplicate measurements of patient samples with both instruments. A13 day evaluation using this system was carried out in both laboratories during all shifts and showed excellent agreement with the manufacturer's reported values. The combined standard deviation for pH at all levels was 0.009,pCO2 -0.83 mmHg and pO2 - 3.9 mmHg. Patients' samples analyzed in the same instrument showed an absolute average difference between duplicates for pH of 0.003, pCO2 - 0.62 mmHg and pO2 -0.87 mmHg. The comparison of duplicate samples in the two instruments in the different laboratories showed an absolute average difference for pH of 0.009,pCO2-1.7mmHg and pO2 - 1.6 mmHg."}
{"id": "PMID:464545", "title": "New kinetic procedure for the measurement of conjugated bilirubin with a centrifugal analyzer.", "content": "A procedure has been developed for the automated measurement of conjugated bilirubin in serum with a centrifugal analyzer. The conjugated bilirubin is measured by a fixed time kinetic method which monitors the reaction between conjugated bilirubin and diazotized sulfanilic acid at 550 nm. Results are calculated based on a comparison of the reagent blank-corrected absorbance changes between 15 seconds and 75 seconds for sample vs changes in an empirical standard. The standard used is N-(1-naphthyl) ethylene diamine dihydrochloride (NEDC) which reacts with diazotized sulfanilic acid at a rate comparable to conjugated bilirubin. The standard is calibrated by comparison with a modified Jendrassik and Grof procedure using a serum blank-corrected centrifugal analyzer reference method. The method is linear to 150 mg per 1 with a sensitivity of 3.0 milliabsorbance units per 1.0 mg per 1 of conjugated bilirubin using a 35 mul sample volume. Within-run precision is 1 percent for elevated concentrations of bilirubin. Hemolysis introduces a negative interference, the nature of which is discussed.", "contents": "New kinetic procedure for the measurement of conjugated bilirubin with a centrifugal analyzer. A procedure has been developed for the automated measurement of conjugated bilirubin in serum with a centrifugal analyzer. The conjugated bilirubin is measured by a fixed time kinetic method which monitors the reaction between conjugated bilirubin and diazotized sulfanilic acid at 550 nm. Results are calculated based on a comparison of the reagent blank-corrected absorbance changes between 15 seconds and 75 seconds for sample vs changes in an empirical standard. The standard used is N-(1-naphthyl) ethylene diamine dihydrochloride (NEDC) which reacts with diazotized sulfanilic acid at a rate comparable to conjugated bilirubin. The standard is calibrated by comparison with a modified Jendrassik and Grof procedure using a serum blank-corrected centrifugal analyzer reference method. The method is linear to 150 mg per 1 with a sensitivity of 3.0 milliabsorbance units per 1.0 mg per 1 of conjugated bilirubin using a 35 mul sample volume. Within-run precision is 1 percent for elevated concentrations of bilirubin. Hemolysis introduces a negative interference, the nature of which is discussed."}
{"id": "PMID:464546", "title": "Estrogen receptors and tumor associated antigens in breast cancer.", "content": "The  methods of performing estrogen and progesterone receptors and the role of receptors in the management of the patient with breast cancer are reviewed. Quality control of the assays and concentrations of receptor protein in normal tissues is emphasized as is the possible relationship of estrogen receptor protein to carcinoembryonic antigen.", "contents": "Estrogen receptors and tumor associated antigens in breast cancer. The  methods of performing estrogen and progesterone receptors and the role of receptors in the management of the patient with breast cancer are reviewed. Quality control of the assays and concentrations of receptor protein in normal tissues is emphasized as is the possible relationship of estrogen receptor protein to carcinoembryonic antigen."}
{"id": "PMID:464550", "title": "Progressive myelopathy due to extramedullary hematopoiesis: case report and review of the literature.", "content": "Extramedullary hematopoiesis (EMH) in the spinal epidural space is a rare but treatable cause of progressive paraparesis in patients with a variety of hematological and systemic disorders. We report a case of extensive thoracic epidural EMH causing progressive spastic paraparesis in a patient with agnogenic myeloid metaplasia. The literature describes 11 additional cases of myelopathy due to epidural EMH. All patients had EMH in the midthoracic region with elevated cerebrospinal fluid protein and abnormal spine roentgenograms or myelograms. Treatment with decompressive laminectomy, radiation therapy, or both, resulted in marked or complete recovery in 7 of 10 patients. Patients who develop paraparesis and who have a disorder associated with EMH should be evaluated rapidly for this treatable condition.", "contents": "Progressive myelopathy due to extramedullary hematopoiesis: case report and review of the literature. Extramedullary hematopoiesis (EMH) in the spinal epidural space is a rare but treatable cause of progressive paraparesis in patients with a variety of hematological and systemic disorders. We report a case of extensive thoracic epidural EMH causing progressive spastic paraparesis in a patient with agnogenic myeloid metaplasia. The literature describes 11 additional cases of myelopathy due to epidural EMH. All patients had EMH in the midthoracic region with elevated cerebrospinal fluid protein and abnormal spine roentgenograms or myelograms. Treatment with decompressive laminectomy, radiation therapy, or both, resulted in marked or complete recovery in 7 of 10 patients. Patients who develop paraparesis and who have a disorder associated with EMH should be evaluated rapidly for this treatable condition."}
{"id": "PMID:464559", "title": "[Pathways of enzymatic inactivation of levomycetin in El Tor vibrios with plasmid and chromosome resistance to the antibiotic].", "content": "Two possible mechanisms of enzymatic inactivation of levomycetin, i.e. acetylation of OH-groups and reduction of the n-nitrophenylic component by the cells and cell-free extracts of V. eltor 2044 with the plasmid or chromosome types of antibiotic resistance were studied in vitro. The vibrio containing the extrachromosome determinants were resistant to a number of antibiotics. The rate of levomycetin acetylation by them under conditions of intensive aeration and reduction of the antibiotic aromatic nitrogroup in the absence of oxygen was high. The cells with the chromosome resistance had a trace activity of levomycetin acetyltransferase. Still, they rather rapidly reduced levomycetin into its aminoderivative (during 2-hour incubation in the atmosphere of nitrogen 70-80% of the substrate are transformed into its summary arylamine). The antibiotic sensitive vibrio practically had no capacity for acetylation of levomycetin but could transform it into the reduced aminoderivative though to a less extent than the antibiotic resistant cells.", "contents": "[Pathways of enzymatic inactivation of levomycetin in El Tor vibrios with plasmid and chromosome resistance to the antibiotic]. Two possible mechanisms of enzymatic inactivation of levomycetin, i.e. acetylation of OH-groups and reduction of the n-nitrophenylic component by the cells and cell-free extracts of V. eltor 2044 with the plasmid or chromosome types of antibiotic resistance were studied in vitro. The vibrio containing the extrachromosome determinants were resistant to a number of antibiotics. The rate of levomycetin acetylation by them under conditions of intensive aeration and reduction of the antibiotic aromatic nitrogroup in the absence of oxygen was high. The cells with the chromosome resistance had a trace activity of levomycetin acetyltransferase. Still, they rather rapidly reduced levomycetin into its aminoderivative (during 2-hour incubation in the atmosphere of nitrogen 70-80% of the substrate are transformed into its summary arylamine). The antibiotic sensitive vibrio practically had no capacity for acetylation of levomycetin but could transform it into the reduced aminoderivative though to a less extent than the antibiotic resistant cells."}
{"id": "PMID:464560", "title": "Comparison of anaerobic susceptibility results obtained by different methods.", "content": "Susceptibility tests using 7 antimicrobial agents (carbenicillin, chloramphenicol, clindamycin, penicillin, cephalothin, metronidazole, and tetracycline) were run against 35 anaerobes including Bacteroides fragilis (17), other gram-negative bacilli (7), clostridia (5), peptococci (4), and eubacteria (2). Results in triplicate obtained by the microbroth dilution method and the aerobic modification of the broth disk method were compared with those obtained with an agar dilution method using Wilkins-Chalgren agar. Media used in the microbroth dilution method included Wilkins-Chalgren broth, brain heart infusion broth, brucella broth, tryptic soy broth, thioglycolate broth, and Schaedler's broth. A result differing by more than one dilution from the Wilkins-Chalgren agar result was considered a discrepancy, and when there was a change in susceptibility status this was termed a significant discrepancy. The microbroth dilution method using Wilkins-Chalgren broth and thioglycolate broth produced the fewest total discrepancies (22 and 24, respectively), and Wilkins-Chalgren broth, thioglycolate, and Schaedler's broth had the fewest significant discrepancies (6, 5, and 5, respectively). With the broth disk method, there were 15 significant discrepancies, although half of these were with tetracycline, which was the antimicrobial agent associated with the highest number of significant discrepancies (33), considering all of the test methods and media.", "contents": "Comparison of anaerobic susceptibility results obtained by different methods. Susceptibility tests using 7 antimicrobial agents (carbenicillin, chloramphenicol, clindamycin, penicillin, cephalothin, metronidazole, and tetracycline) were run against 35 anaerobes including Bacteroides fragilis (17), other gram-negative bacilli (7), clostridia (5), peptococci (4), and eubacteria (2). Results in triplicate obtained by the microbroth dilution method and the aerobic modification of the broth disk method were compared with those obtained with an agar dilution method using Wilkins-Chalgren agar. Media used in the microbroth dilution method included Wilkins-Chalgren broth, brain heart infusion broth, brucella broth, tryptic soy broth, thioglycolate broth, and Schaedler's broth. A result differing by more than one dilution from the Wilkins-Chalgren agar result was considered a discrepancy, and when there was a change in susceptibility status this was termed a significant discrepancy. The microbroth dilution method using Wilkins-Chalgren broth and thioglycolate broth produced the fewest total discrepancies (22 and 24, respectively), and Wilkins-Chalgren broth, thioglycolate, and Schaedler's broth had the fewest significant discrepancies (6, 5, and 5, respectively). With the broth disk method, there were 15 significant discrepancies, although half of these were with tetracycline, which was the antimicrobial agent associated with the highest number of significant discrepancies (33), considering all of the test methods and media."}
{"id": "PMID:464561", "title": "Avermectins, new family of potent anthelmintic agents: producing organism and fermentation.", "content": "The avermectins are a complex of chemically related agents which exhibit extraordinarily potent anthelmintic activity. They are produced by a novel species of actinomycete, NRRL 8165, which we have named Streptomyces avermitilis. The morphological and cultural characteristics which differentiate the producing organism from other species are described. The avermectins have been identified as a series of macrocyclic lactone derivatives which, in contrast to the macrolide or polyene antibiotics, lack significant antibacterial or antifungal activity. The avermectin complex is fully active against the gastrointestinal nematode Nematospiroides dubius when fed to infected mice for 6 days at 0.0002% of the diet. Fermentation development, including medium modification and strain selection, resulted in increasing the broth yields from 9 to 500 mug/ml.", "contents": "Avermectins, new family of potent anthelmintic agents: producing organism and fermentation. The avermectins are a complex of chemically related agents which exhibit extraordinarily potent anthelmintic activity. They are produced by a novel species of actinomycete, NRRL 8165, which we have named Streptomyces avermitilis. The morphological and cultural characteristics which differentiate the producing organism from other species are described. The avermectins have been identified as a series of macrocyclic lactone derivatives which, in contrast to the macrolide or polyene antibiotics, lack significant antibacterial or antifungal activity. The avermectin complex is fully active against the gastrointestinal nematode Nematospiroides dubius when fed to infected mice for 6 days at 0.0002% of the diet. Fermentation development, including medium modification and strain selection, resulted in increasing the broth yields from 9 to 500 mug/ml."}
{"id": "PMID:464562", "title": "Avermectins, new family of potent anthelmintic agents: isolation and chromatographic properties.", "content": "The avermectins, a family of new anthelmintic agents, were isolated from the mycelia of Streptomyces avermitilis. Four closely related major components and four homologous minor components were separated from the complex. Solvent extraction, solvent partition, and adsorption methods were used to isolate and purify the complex; novel partition chromatography systems using Sephadex LH-20 were used to separate the components. A reverse-phase high-pressure liquid chromatography assay for the quantitative determination of all components was used extensively to monitor the purification methods.", "contents": "Avermectins, new family of potent anthelmintic agents: isolation and chromatographic properties. The avermectins, a family of new anthelmintic agents, were isolated from the mycelia of Streptomyces avermitilis. Four closely related major components and four homologous minor components were separated from the complex. Solvent extraction, solvent partition, and adsorption methods were used to isolate and purify the complex; novel partition chromatography systems using Sephadex LH-20 were used to separate the components. A reverse-phase high-pressure liquid chromatography assay for the quantitative determination of all components was used extensively to monitor the purification methods."}
{"id": "PMID:464563", "title": "Avermectins, new family of potent anthelmintic agents: efficacy of the B1a component.", "content": "When given to sheep as a single oral dose at 0.1 mg/kg, the B(1a) component of the avermectins caused a reduction of >95% in the numbers of Haemonchus contortus, Ostertagia circumcincta (including inhibited L(4) larvae), Trichostrongylus axei, Trichostrongylus colubriformis, Cooperia oncophora, and Oesophagostomum columbianum. When given to cattle as a single oral dose at 0.1 mg/kg, avermectin B(1a) was >95% effective in reducing the numbers of Haemonchus placei, Ostertagia ostertagi (including inhibited L(4) larvae), T. axei, T. colubriformis, C. oncophora, Cooperia punctata, Oesophagostomum radiatum, and Dictyocaulus viviparus. Avermectin B(1a) was similarly effective, with the exception of a detectable loss in activity against adult C. oncophora, when administered to cattle as a parenteral injection. Some of these ruminant parasites were fully susceptible to dosages of avermectin B(1a) at 0.025 mg/kg, e.g., D. viviparus, O. radiatum, O. ostertagi, and H. contortus. Avermectin B(1a) removed 83 to 100% of Ancylostoma caninum from dogs given a single oral dose of 0.003 to 0.005 mg/kg. The poultry nematodes Capillaria obsignata and immature Ascaridia galli were effectively removed by avermectin B(1a) at 0.05 and 0.1 mg/kg, respectively, but 0.1 mg/kg was not effective for Heterakis gallinarum. Thus, the avermectins would appear to have unprecedented potency and spectrum of biological activity.", "contents": "Avermectins, new family of potent anthelmintic agents: efficacy of the B1a component. When given to sheep as a single oral dose at 0.1 mg/kg, the B(1a) component of the avermectins caused a reduction of >95% in the numbers of Haemonchus contortus, Ostertagia circumcincta (including inhibited L(4) larvae), Trichostrongylus axei, Trichostrongylus colubriformis, Cooperia oncophora, and Oesophagostomum columbianum. When given to cattle as a single oral dose at 0.1 mg/kg, avermectin B(1a) was >95% effective in reducing the numbers of Haemonchus placei, Ostertagia ostertagi (including inhibited L(4) larvae), T. axei, T. colubriformis, C. oncophora, Cooperia punctata, Oesophagostomum radiatum, and Dictyocaulus viviparus. Avermectin B(1a) was similarly effective, with the exception of a detectable loss in activity against adult C. oncophora, when administered to cattle as a parenteral injection. Some of these ruminant parasites were fully susceptible to dosages of avermectin B(1a) at 0.025 mg/kg, e.g., D. viviparus, O. radiatum, O. ostertagi, and H. contortus. Avermectin B(1a) removed 83 to 100% of Ancylostoma caninum from dogs given a single oral dose of 0.003 to 0.005 mg/kg. The poultry nematodes Capillaria obsignata and immature Ascaridia galli were effectively removed by avermectin B(1a) at 0.05 and 0.1 mg/kg, respectively, but 0.1 mg/kg was not effective for Heterakis gallinarum. Thus, the avermectins would appear to have unprecedented potency and spectrum of biological activity."}
{"id": "PMID:464564", "title": "Quantitative nasal cultures from carriers of Staphylococcus aureus: effects of oral therapy with erythromycin, rosamicin, and placebo.", "content": "Serial quantitative nasal cultures were performed on 87 healthy nasal carriers of Staphylococcus aureus, who were randomly assigned to 7 days of oral therapy with erythromycin, rosamicin (an investigational macrolide antibiotic), or placebo. Staphylococcal carrier rates decreased during therapy with both antibiotics; however, erythromycin was significantly more effective in lowering carrier rates than was rosamicin. The anti-staphylococcal effects of both antibiotics were similar when the mean numbers of S. aureus isolated from positive cultures during therapy were compared. Side effects to each regimen were minimal.", "contents": "Quantitative nasal cultures from carriers of Staphylococcus aureus: effects of oral therapy with erythromycin, rosamicin, and placebo. Serial quantitative nasal cultures were performed on 87 healthy nasal carriers of Staphylococcus aureus, who were randomly assigned to 7 days of oral therapy with erythromycin, rosamicin (an investigational macrolide antibiotic), or placebo. Staphylococcal carrier rates decreased during therapy with both antibiotics; however, erythromycin was significantly more effective in lowering carrier rates than was rosamicin. The anti-staphylococcal effects of both antibiotics were similar when the mean numbers of S. aureus isolated from positive cultures during therapy were compared. Side effects to each regimen were minimal."}
{"id": "PMID:464565", "title": "Antibiotic action of N-methylthioformohydroxamate metal complexes.", "content": "The antibiotic activity of metal complexes of N-methylthioformohydroxamic acid against gram-negative Escherichia coli NIHJ and gram-positive Staphylococcus aureus 209P was investigated. The kinetically labile, square-planar, divalent (Cu, Ni, and Pd) and octahedral, trivalent (Fe, Co, and Cr) complexes displayed activity, whereas the more inert platinum(II) or rhodium(III) complex displayed no activity, or activity only at elevated concentrations. The free ligand did not suppress the growth of the above organisms, and the sulfur atom of the ligand in its metal complexes appears crucial for activity. Uptake studies of radioactively labeled N-methylthioformohydroxamic acid, its nickel(II), platinum(II), iron(III), and rhodium(III) complexes were conducted in the Escherichia coli K-12 RW193 mutant, which is defective in the production of its native iron(III) transport agent, enterobactin. Uptake of (55)Fe or (63)Ni label from their metal complexes appeared to occur as free inorganic metal ions. The antibiotic activity of the iron(III) and nickel(II) complexes was not due merely to an enhanced accumulation of metal ions by the cell. Metal complexes of [(35)S]N-methylthioformohydroxamic acid enhanced the accumulation of (35)S label compared to that of the free ligand. The antibiotic activity and uptake data are discussed in terms of possible modes of action.", "contents": "Antibiotic action of N-methylthioformohydroxamate metal complexes. The antibiotic activity of metal complexes of N-methylthioformohydroxamic acid against gram-negative Escherichia coli NIHJ and gram-positive Staphylococcus aureus 209P was investigated. The kinetically labile, square-planar, divalent (Cu, Ni, and Pd) and octahedral, trivalent (Fe, Co, and Cr) complexes displayed activity, whereas the more inert platinum(II) or rhodium(III) complex displayed no activity, or activity only at elevated concentrations. The free ligand did not suppress the growth of the above organisms, and the sulfur atom of the ligand in its metal complexes appears crucial for activity. Uptake studies of radioactively labeled N-methylthioformohydroxamic acid, its nickel(II), platinum(II), iron(III), and rhodium(III) complexes were conducted in the Escherichia coli K-12 RW193 mutant, which is defective in the production of its native iron(III) transport agent, enterobactin. Uptake of (55)Fe or (63)Ni label from their metal complexes appeared to occur as free inorganic metal ions. The antibiotic activity of the iron(III) and nickel(II) complexes was not due merely to an enhanced accumulation of metal ions by the cell. Metal complexes of [(35)S]N-methylthioformohydroxamic acid enhanced the accumulation of (35)S label compared to that of the free ligand. The antibiotic activity and uptake data are discussed in terms of possible modes of action."}
{"id": "PMID:464566", "title": "In vitro activity of 39 antimicrobial agents against Treponema hyodysenteriae.", "content": "The in vitro activities of 39 antimicrobial agents against 23 isolates of Treponema hyodysenteriae, the majority of which were field isolates, were determined by the agar dilution technique. Quinoxalines, pleuromutilin, nitroimidazoles, and nitrofuran were the most active. Their activities ranged from </=0.10 to 1.56 mug/ml. Lincomycin, penicillins, chloramphenicol, tetracyclines, cephalosporins, three peptides (virginiamycin, thiopeptin, and bacitracin), and one aminoglycoside (gentamicin) exhibited intermediate levels of activity ranging from 0.39 to 50 mug/ml. Four peptides (enduracidin, viomycin, bicyclomycin, and colistin), three aminoglycosides (kanamycin, streptomycin, and neomycin), polyene, and other agents, including novobiocin, vancomycin, rifampin, nalidixic acid, and p-arsanilic acid, displayed limited activities ranging from 12.5 to >/=100 mug/ml. Macrolides showed varying degrees of activity depending upon isolates.", "contents": "In vitro activity of 39 antimicrobial agents against Treponema hyodysenteriae. The in vitro activities of 39 antimicrobial agents against 23 isolates of Treponema hyodysenteriae, the majority of which were field isolates, were determined by the agar dilution technique. Quinoxalines, pleuromutilin, nitroimidazoles, and nitrofuran were the most active. Their activities ranged from </=0.10 to 1.56 mug/ml. Lincomycin, penicillins, chloramphenicol, tetracyclines, cephalosporins, three peptides (virginiamycin, thiopeptin, and bacitracin), and one aminoglycoside (gentamicin) exhibited intermediate levels of activity ranging from 0.39 to 50 mug/ml. Four peptides (enduracidin, viomycin, bicyclomycin, and colistin), three aminoglycosides (kanamycin, streptomycin, and neomycin), polyene, and other agents, including novobiocin, vancomycin, rifampin, nalidixic acid, and p-arsanilic acid, displayed limited activities ranging from 12.5 to >/=100 mug/ml. Macrolides showed varying degrees of activity depending upon isolates."}
{"id": "PMID:464567", "title": "Interpretation of the disk diffusion susceptibility test for amikacin: report of a collaborative study.", "content": "Because excessively high rates of false resistance have been encountered with the 10-mug amikacin disk in diffusion susceptibility tests, a study was performed to examine existing zone diameter interpretative criteria and to compare the accuracy of 10- and 30-mug amikacin disks by the error rate-bounded classification scheme. Although current zone diameter interpretative criteria eliminate false susceptibles, there is an unacceptably high rate of false resistants. This problem can be resolved in most instances by revising the zone diameter interpretative criteria for the 10-mug disk (resistant, </=9 mm; indeterminate, 10 to 11 mm; susceptible, >/=12 mm) or, preferably, by replacing the 10-mug disk with a 30-mug disk and adopting new interpretative criteria (resistant, </=14 mm; indeterminate, 15 to 16 mm; susceptible, >/=17 mm). Because of significant differences in performance among media, it is necessary to include Pseudomonas aeruginosa ATCC 27853 among controls routinely tested and to exclude from use lots of Mueller-Hinton agar yielding results outside the 75% tolerance (90% confidence) limits for amikacin.", "contents": "Interpretation of the disk diffusion susceptibility test for amikacin: report of a collaborative study. Because excessively high rates of false resistance have been encountered with the 10-mug amikacin disk in diffusion susceptibility tests, a study was performed to examine existing zone diameter interpretative criteria and to compare the accuracy of 10- and 30-mug amikacin disks by the error rate-bounded classification scheme. Although current zone diameter interpretative criteria eliminate false susceptibles, there is an unacceptably high rate of false resistants. This problem can be resolved in most instances by revising the zone diameter interpretative criteria for the 10-mug disk (resistant, </=9 mm; indeterminate, 10 to 11 mm; susceptible, >/=12 mm) or, preferably, by replacing the 10-mug disk with a 30-mug disk and adopting new interpretative criteria (resistant, </=14 mm; indeterminate, 15 to 16 mm; susceptible, >/=17 mm). Because of significant differences in performance among media, it is necessary to include Pseudomonas aeruginosa ATCC 27853 among controls routinely tested and to exclude from use lots of Mueller-Hinton agar yielding results outside the 75% tolerance (90% confidence) limits for amikacin."}
{"id": "PMID:464568", "title": "In vitro evaluation of seven cationic detergents as antiplaque agents.", "content": "The bactericidal efficacy of seven cationic detergents was studied in vitro against intact performed plaques of representative strains of four oral dental plaque-forming microorganisms: Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, and Actinomyces naeslundii. Both absolute (plaque bactericidal index) and relative (chlorhexidine coefficient) indexes of antiplaque activity were established. Only cetyltrimethylammonium bromide was more efficacious than the reference agent, the bisbiguanide chlorhexidine, for the apparently more potent tooth-adherent cariogenic and periodontopathic elements, respectively, of the in vivo plaque microflora, S. mutans and A. viscosus.", "contents": "In vitro evaluation of seven cationic detergents as antiplaque agents. The bactericidal efficacy of seven cationic detergents was studied in vitro against intact performed plaques of representative strains of four oral dental plaque-forming microorganisms: Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, and Actinomyces naeslundii. Both absolute (plaque bactericidal index) and relative (chlorhexidine coefficient) indexes of antiplaque activity were established. Only cetyltrimethylammonium bromide was more efficacious than the reference agent, the bisbiguanide chlorhexidine, for the apparently more potent tooth-adherent cariogenic and periodontopathic elements, respectively, of the in vivo plaque microflora, S. mutans and A. viscosus."}
{"id": "PMID:464569", "title": "Large-scale production and concentration of human lymphoid interferon.", "content": "A stable and predictable production system is described for pilot plant quantities (milligram) of human lymphoid interferon, using suspension culture of an African Burkitt's lymphoma derived cell line Namalva with induction by Newcastle disease virus, B-1 strain. Cell cultures were grown in impeller-driven 50-liter fermentors with dilution of the postinduction culture using serum-free medium. High levels of dissolved oxygen were necessary for optimum cell growth. A total of 4,207 liters of interferon culture was produced in a series of 116 fermentor runs. An average yield of 3.5 log(10) international units of interferon per ml was realized before processing. Trichloroacetic acid was used to precipitate the interferon. An average of 3.35 log(10) international units of interferon per ml was recovered in the final nonpurified product.", "contents": "Large-scale production and concentration of human lymphoid interferon. A stable and predictable production system is described for pilot plant quantities (milligram) of human lymphoid interferon, using suspension culture of an African Burkitt's lymphoma derived cell line Namalva with induction by Newcastle disease virus, B-1 strain. Cell cultures were grown in impeller-driven 50-liter fermentors with dilution of the postinduction culture using serum-free medium. High levels of dissolved oxygen were necessary for optimum cell growth. A total of 4,207 liters of interferon culture was produced in a series of 116 fermentor runs. An average yield of 3.5 log(10) international units of interferon per ml was realized before processing. Trichloroacetic acid was used to precipitate the interferon. An average of 3.35 log(10) international units of interferon per ml was recovered in the final nonpurified product."}
{"id": "PMID:464570", "title": "Purification of a streptococcal bacteriocin (viridin B) and its separation from alpha-hemolysin.", "content": "Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.", "contents": "Purification of a streptococcal bacteriocin (viridin B) and its separation from alpha-hemolysin. Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000."}
{"id": "PMID:464571", "title": "Antibiotic susceptibility of Streptococcus mutans: comparison of serotype profiles.", "content": "A total of 82 strains of Streptococcus mutans representing serotypes a through g were tested for susceptibility to erythromycin, penicillin, methicillin, lincomycin, tetracycline, vancomycin, gentamicin, streptomycin, neomycin, kanamycin, bacitracin, and polymyxin B. Strains included stock cultures and isolates from human and animal dental plaque. Minimal inhibitory concentrations were determined by a broth-microdilution procedure. The major differences in antibiotic susceptibility observed among the serotypes resulted with antibiotics which act on the cell surface. Bacitracin was most active against serotype a strains and polymyxin B against serotype b strains. Serotypes a, d, and g were less susceptible than the other serotypes to methicillin.", "contents": "Antibiotic susceptibility of Streptococcus mutans: comparison of serotype profiles. A total of 82 strains of Streptococcus mutans representing serotypes a through g were tested for susceptibility to erythromycin, penicillin, methicillin, lincomycin, tetracycline, vancomycin, gentamicin, streptomycin, neomycin, kanamycin, bacitracin, and polymyxin B. Strains included stock cultures and isolates from human and animal dental plaque. Minimal inhibitory concentrations were determined by a broth-microdilution procedure. The major differences in antibiotic susceptibility observed among the serotypes resulted with antibiotics which act on the cell surface. Bacitracin was most active against serotype a strains and polymyxin B against serotype b strains. Serotypes a, d, and g were less susceptible than the other serotypes to methicillin."}
{"id": "PMID:464572", "title": "Clinical pharmacology of intravenously administered trimethoprim-sulfamethoxazole.", "content": "Pharmacokinetic studies of intravenously administered trimethoprim-sulfamethoxazole (TMP-SMX) were conducted in 11 patients with cancer while they received therapy with this drug combination for infection. Each patient received 160 mg of TMP and 800 mg of SMX every 8 h. The highest plasma concentrations of both agents were attained at the end of a 1-h infusion period, and the levels were maintained above 38 mug of free SMX and 2 mug of TMP per ml for 2 to 4 h on day 1. On day 4, these concentrations were exceeded at all time intervals of blood sampling. High concentrations of TMP and free SMX were recovered in the urine during the 8-h period. The plasma half-lives of TMP and free SMX, as determined during the first 8-h period, were 7.6 and 8.6 h, respectively. Compared with SMX, TMP had an approximately 2.5 times higher volume of distribution. This drug combination was well tolerated by the patients and unaccompanied by drug-related toxicity.", "contents": "Clinical pharmacology of intravenously administered trimethoprim-sulfamethoxazole. Pharmacokinetic studies of intravenously administered trimethoprim-sulfamethoxazole (TMP-SMX) were conducted in 11 patients with cancer while they received therapy with this drug combination for infection. Each patient received 160 mg of TMP and 800 mg of SMX every 8 h. The highest plasma concentrations of both agents were attained at the end of a 1-h infusion period, and the levels were maintained above 38 mug of free SMX and 2 mug of TMP per ml for 2 to 4 h on day 1. On day 4, these concentrations were exceeded at all time intervals of blood sampling. High concentrations of TMP and free SMX were recovered in the urine during the 8-h period. The plasma half-lives of TMP and free SMX, as determined during the first 8-h period, were 7.6 and 8.6 h, respectively. Compared with SMX, TMP had an approximately 2.5 times higher volume of distribution. This drug combination was well tolerated by the patients and unaccompanied by drug-related toxicity."}
{"id": "PMID:464573", "title": "Role of vancomycin as a component of oral nonabsorbable antibiotics for microbial suppression in leukemic patients.", "content": "A total of 38 adult patients with acute leukemia who were undergoing remission induction chemotherapy in regular patient rooms were randomly allocated to one of two oral nonabsorbable antibiotic regimens for infection prophylaxis (gentamicin, vancomycin, and nystatin [GVN] or gentamicin and nystatin [GN]) to evaluate whether vancomycin was a necessary component. The patient population in both groups were comparable. Tolerance to GVN was less than GN but compliance was approximately equal (>85% in both groups). Patients receiving vancomycin demonstrated greater overall alimentary tract microbial suppression; however, acquisition of potential pathogens was approximately equal in both groups. The incidence of bacteremia, as well as the overall incidence of infection as related to the number of days at various granulocyte levels, was also approximately equal in both groups. Group D Streptococcus species were poorly suppressed by GN compared with GVN, although no patient developed an infection with these organisms. Colonization by newly acquired gram-negative bacilli was significantly less in the GN group (GN, 3 colonizations; GVN, 13 colonizations; P < 0.01). It is concluded that vancomycin may be safely eliminated from the GVN regimen provided microbiological data is monitored to detect resistant organisms.", "contents": "Role of vancomycin as a component of oral nonabsorbable antibiotics for microbial suppression in leukemic patients. A total of 38 adult patients with acute leukemia who were undergoing remission induction chemotherapy in regular patient rooms were randomly allocated to one of two oral nonabsorbable antibiotic regimens for infection prophylaxis (gentamicin, vancomycin, and nystatin [GVN] or gentamicin and nystatin [GN]) to evaluate whether vancomycin was a necessary component. The patient population in both groups were comparable. Tolerance to GVN was less than GN but compliance was approximately equal (>85% in both groups). Patients receiving vancomycin demonstrated greater overall alimentary tract microbial suppression; however, acquisition of potential pathogens was approximately equal in both groups. The incidence of bacteremia, as well as the overall incidence of infection as related to the number of days at various granulocyte levels, was also approximately equal in both groups. Group D Streptococcus species were poorly suppressed by GN compared with GVN, although no patient developed an infection with these organisms. Colonization by newly acquired gram-negative bacilli was significantly less in the GN group (GN, 3 colonizations; GVN, 13 colonizations; P < 0.01). It is concluded that vancomycin may be safely eliminated from the GVN regimen provided microbiological data is monitored to detect resistant organisms."}
{"id": "PMID:464574", "title": "Photochemical inactivation of deoxyribonucleic and ribonucleic acid viruses by chlorpromazine.", "content": "Chlorpromazine, a widely used tranquilizing drug of the phenothiazine group, was found to be a very potent photochemical inactivator of both deoxyribonucleic acid and ribonucleic acid viruses in the presence of long-wave ultraviolet light (320 to 380 nm). Neither the light alone nor chlorpromazine alone caused any appreciable inactivation. The known chlorpromazine photoreactions with nucleic acids are somewhat similar to those of psoralen (furocoumarin) derivatives. As in the case of the psoralens, chlorpromazine is capable of photoinactivating viruses totally within a few minutes under near-physiological or other gentle conditions. The antiviral effects of the chlorpromazine photoreaction could make it valuable for the development of inactivated viral vaccines as well as for use in the photochemotherapy of viral dermatoses.", "contents": "Photochemical inactivation of deoxyribonucleic and ribonucleic acid viruses by chlorpromazine. Chlorpromazine, a widely used tranquilizing drug of the phenothiazine group, was found to be a very potent photochemical inactivator of both deoxyribonucleic acid and ribonucleic acid viruses in the presence of long-wave ultraviolet light (320 to 380 nm). Neither the light alone nor chlorpromazine alone caused any appreciable inactivation. The known chlorpromazine photoreactions with nucleic acids are somewhat similar to those of psoralen (furocoumarin) derivatives. As in the case of the psoralens, chlorpromazine is capable of photoinactivating viruses totally within a few minutes under near-physiological or other gentle conditions. The antiviral effects of the chlorpromazine photoreaction could make it valuable for the development of inactivated viral vaccines as well as for use in the photochemotherapy of viral dermatoses."}
{"id": "PMID:464575", "title": "Influence of probenecid on serum levels and urinary excretion of cinoxacin.", "content": "Serum levels and urinary excretion of cinoxacin were examined in healthy individuals after a two-step intravenous infusion in the presence and absence of probenecid. After dosing cinoxacin alone, steady-state serum levels were approached in 1 h and were maintained for an additional 2 h with a reduced infusion rate. After probenecid pretreatment, serum levels of cinoxacin continued to increase during 3 h of infusion, reaching levels approximately double those obtained with cinoxacin alone. The mean elimination half-life of cinoxacin from serum was increased from 1.3 to 3.5 h in the presence of probenecid, and renal clearance was significantly reduced, with 46% of dosed drug appearing in 7-h urines of probenecid-treated subjects compared with 68% in subjects receiving cinoxacin alone. Probenecid had no apparent influence on cinoxacin distribution in the body but caused a significant decrease in the rate of cinoxacin extrarenal elimination, possibly due to competition for a common metabolic pathway.", "contents": "Influence of probenecid on serum levels and urinary excretion of cinoxacin. Serum levels and urinary excretion of cinoxacin were examined in healthy individuals after a two-step intravenous infusion in the presence and absence of probenecid. After dosing cinoxacin alone, steady-state serum levels were approached in 1 h and were maintained for an additional 2 h with a reduced infusion rate. After probenecid pretreatment, serum levels of cinoxacin continued to increase during 3 h of infusion, reaching levels approximately double those obtained with cinoxacin alone. The mean elimination half-life of cinoxacin from serum was increased from 1.3 to 3.5 h in the presence of probenecid, and renal clearance was significantly reduced, with 46% of dosed drug appearing in 7-h urines of probenecid-treated subjects compared with 68% in subjects receiving cinoxacin alone. Probenecid had no apparent influence on cinoxacin distribution in the body but caused a significant decrease in the rate of cinoxacin extrarenal elimination, possibly due to competition for a common metabolic pathway."}
{"id": "PMID:464576", "title": "Comparison of microdilution and broth dilution techniques for the susceptibility testing of yeasts to 5-fluorocytosine and amphotericin B.", "content": "Microdilution and broth dilution techniques were compared for the susceptibility testing of 50 clinical yeast isolates to 5-fluorocytosine and amphotericin B. Good correlation between methods was obtained with all isolates except Cryptococcus neoformans.", "contents": "Comparison of microdilution and broth dilution techniques for the susceptibility testing of yeasts to 5-fluorocytosine and amphotericin B. Microdilution and broth dilution techniques were compared for the susceptibility testing of 50 clinical yeast isolates to 5-fluorocytosine and amphotericin B. Good correlation between methods was obtained with all isolates except Cryptococcus neoformans."}
{"id": "PMID:464577", "title": "Pitfalls in collecting blood specimens for antibiotic assay.", "content": "Obtaining blood samples for antibiotic analysis from intravenous lines through which the antibiotic had been administered was critically evaluated. Flushing volumes required to remove contaminating antibiotic varied with the type of antibiotic and with different plastics. Obtaining blood specimens from the intravenous lines is not practical in infants and not advised in adults.", "contents": "Pitfalls in collecting blood specimens for antibiotic assay. Obtaining blood samples for antibiotic analysis from intravenous lines through which the antibiotic had been administered was critically evaluated. Flushing volumes required to remove contaminating antibiotic varied with the type of antibiotic and with different plastics. Obtaining blood specimens from the intravenous lines is not practical in infants and not advised in adults."}
{"id": "PMID:464578", "title": "In vitro activity of mezlocillin and its related compounds against aerobic and anaerobic bacteria.", "content": "A total of 900 clinical isolates of aerobic (462 isolates) and anaerobic (438 isolates) bacteria were tested against mezlocillin in comparison with other penicillins, and the aerobes were also tested against cephalothin, cefoxitin, and cefamandole. Among penicillins, mezlocillin was the most effective against Escherichia coli, Klebsiella pneumoniae, Salmonella species, Pseudomonas aeruginosa, and Bacteroides fragilis. Mezlocillin was more effective than cephalothin against Klebsiella pneumoniae.", "contents": "In vitro activity of mezlocillin and its related compounds against aerobic and anaerobic bacteria. A total of 900 clinical isolates of aerobic (462 isolates) and anaerobic (438 isolates) bacteria were tested against mezlocillin in comparison with other penicillins, and the aerobes were also tested against cephalothin, cefoxitin, and cefamandole. Among penicillins, mezlocillin was the most effective against Escherichia coli, Klebsiella pneumoniae, Salmonella species, Pseudomonas aeruginosa, and Bacteroides fragilis. Mezlocillin was more effective than cephalothin against Klebsiella pneumoniae."}
{"id": "PMID:464579", "title": "High-pressure liquid chromatographic assay of chloramphenicol in biological fluids.", "content": "Chloramphenicol can be assayed by a sensitive and selective high-pressure liquid chromatographic assay technique. The method described is easily adapted to analysis of many other drugs.", "contents": "High-pressure liquid chromatographic assay of chloramphenicol in biological fluids. Chloramphenicol can be assayed by a sensitive and selective high-pressure liquid chromatographic assay technique. The method described is easily adapted to analysis of many other drugs."}
{"id": "PMID:464580", "title": "Antimicrobial susceptibilities of rough, smooth, and mucoid colony types of Pseudomonas isolated from cystic fibrosis patients.", "content": "Minimal inhibitory concentrations of carbenicillin, chloramphenicol, tetracycline, gentamicin, tobramycin, and trimethoprim-sulfamethoxazole were determined for rough, smooth, and mucoid Pseudomonas sp. isolates from sputa collected from 40 cystic fibrosis patients. Ninety-four percent of the minimal inhibitory concentrations obtained by using mixed inocula of colonies of different morphological types fell within one serial dilution of the most resistant minimal inhibitory concentration obtained when each colony type was tested separately.", "contents": "Antimicrobial susceptibilities of rough, smooth, and mucoid colony types of Pseudomonas isolated from cystic fibrosis patients. Minimal inhibitory concentrations of carbenicillin, chloramphenicol, tetracycline, gentamicin, tobramycin, and trimethoprim-sulfamethoxazole were determined for rough, smooth, and mucoid Pseudomonas sp. isolates from sputa collected from 40 cystic fibrosis patients. Ninety-four percent of the minimal inhibitory concentrations obtained by using mixed inocula of colonies of different morphological types fell within one serial dilution of the most resistant minimal inhibitory concentration obtained when each colony type was tested separately."}
{"id": "PMID:464581", "title": "Dissociated resistance among cephalosporins.", "content": "When disks containing cefuroxime (CXM) or HR 756 (756) were placed next to cefoxitin (FOX) on plates inoculated with Enterobacter resistant to FOX, there was marked flattening of the zones of inhibition produced by CXM or 756, but colonies growing near the CXM or 756 disks were found to be fully susceptible to these drugs on retesting. In contrast, when a checkerboard titration with CXM or 756 and FOX was done in broth, organisms growing in the presence of high concentrations of either of the former plus FOX were found to be resistant on retesting. Broth cultures of Enterobacter were found to contain small numbers of mutants which were resistant to and inactivated CXM and 756. Evidence is presented suggesting that the addition of FOX induces similar properties in the whole culture, which then becomes resistant to all of the nine cephalosporins tested and to four of six penicillins tested. Such cultures rapidly reverted to susceptible when FOX was removed or inactivated, and an explanation is offered as to why this did not happen in fluid media.", "contents": "Dissociated resistance among cephalosporins. When disks containing cefuroxime (CXM) or HR 756 (756) were placed next to cefoxitin (FOX) on plates inoculated with Enterobacter resistant to FOX, there was marked flattening of the zones of inhibition produced by CXM or 756, but colonies growing near the CXM or 756 disks were found to be fully susceptible to these drugs on retesting. In contrast, when a checkerboard titration with CXM or 756 and FOX was done in broth, organisms growing in the presence of high concentrations of either of the former plus FOX were found to be resistant on retesting. Broth cultures of Enterobacter were found to contain small numbers of mutants which were resistant to and inactivated CXM and 756. Evidence is presented suggesting that the addition of FOX induces similar properties in the whole culture, which then becomes resistant to all of the nine cephalosporins tested and to four of six penicillins tested. Such cultures rapidly reverted to susceptible when FOX was removed or inactivated, and an explanation is offered as to why this did not happen in fluid media."}
{"id": "PMID:464582", "title": "Purification, properties, and cytotoxic effect of a bacteriocin from Mycobacterium smegmatis.", "content": "The bacteriocin produced by Mycobacterium smegmatis ATCC 14468 was isolated, and a study was made of its chemical, physical, and biological properties. No appreciable bacteriocin activity was found in the culture supernatant fluids, but it was released in appreciable quantities after disruption of the cells. The material was purified 49-fold by means of chromatography on diethylaminoethyl-cellulose, ammonium sulfate fractionation, gel filtration on Sephadex G-200, and chromatography on diethylaminoethyl-Sephadex A-50. Its molecular weight was determined to be approximately 75,000 from the elution profile on Sephadex G-200 chromatography. The bacteriocin was resistant to deoxyribonuclease, ribonuclease, lipase, ultraviolet irradiation, and freeze-thawing, whereas it was relatively less thermostable and was sensitive to proteolytic enzymes. The lethal effect of the bacteriocin was demonstrated by the decrease in viable counts of the bacteriocin-sensitive indicator strain, M. diernhoferi ATCC 19340. The bacteriocin preparation inhibited the growth of HeLa-S3 cells.", "contents": "Purification, properties, and cytotoxic effect of a bacteriocin from Mycobacterium smegmatis. The bacteriocin produced by Mycobacterium smegmatis ATCC 14468 was isolated, and a study was made of its chemical, physical, and biological properties. No appreciable bacteriocin activity was found in the culture supernatant fluids, but it was released in appreciable quantities after disruption of the cells. The material was purified 49-fold by means of chromatography on diethylaminoethyl-cellulose, ammonium sulfate fractionation, gel filtration on Sephadex G-200, and chromatography on diethylaminoethyl-Sephadex A-50. Its molecular weight was determined to be approximately 75,000 from the elution profile on Sephadex G-200 chromatography. The bacteriocin was resistant to deoxyribonuclease, ribonuclease, lipase, ultraviolet irradiation, and freeze-thawing, whereas it was relatively less thermostable and was sensitive to proteolytic enzymes. The lethal effect of the bacteriocin was demonstrated by the decrease in viable counts of the bacteriocin-sensitive indicator strain, M. diernhoferi ATCC 19340. The bacteriocin preparation inhibited the growth of HeLa-S3 cells."}
{"id": "PMID:464583", "title": "Bacampicillin, Ampicillin, Cephalothin, and Cephapirin levels in human blood and interstitial fluid.", "content": "The diffusibility of bacampicillin, ampicillin, cephalothin, and cephapirin into human interstitial fluid was investigated by using crossover studies. We compared bacampicillin with ampicillin and found that bacampicillin was better absorbed after oral administration. Blood, interstitial fluid, and urine levels were consistently higher in volunteers who received bacampicillin. We compared cephalothin with cephapirin and found that blood and interstitial fluid levels were comparable throughout the study.", "contents": "Bacampicillin, Ampicillin, Cephalothin, and Cephapirin levels in human blood and interstitial fluid. The diffusibility of bacampicillin, ampicillin, cephalothin, and cephapirin into human interstitial fluid was investigated by using crossover studies. We compared bacampicillin with ampicillin and found that bacampicillin was better absorbed after oral administration. Blood, interstitial fluid, and urine levels were consistently higher in volunteers who received bacampicillin. We compared cephalothin with cephapirin and found that blood and interstitial fluid levels were comparable throughout the study."}
{"id": "PMID:464584", "title": "Sporulation and the production of serine protease and cephamycin C by Streptomyces lactamdurans.", "content": "Streptomyces lactamdurans, producer of the antibiotic cephamycin C, excretes at least two proteases. Physiological studies indicate that antibiotic synthesis and serine protease formation are coordinately regulated. Both are produced only after culture growth ends, and they appear with essentially identical kinetics. In addition, strains which produce superior levels of cephamycin C form equally superior levels of the serine protease. Genetic evidence reveals that the syntheses of the antibiotic and serine proteases are associated with sporulation. Mutants which fail to produce aerial hyphae (bald mutants) also fail to synthesize the antibiotic and serine proteases.", "contents": "Sporulation and the production of serine protease and cephamycin C by Streptomyces lactamdurans. Streptomyces lactamdurans, producer of the antibiotic cephamycin C, excretes at least two proteases. Physiological studies indicate that antibiotic synthesis and serine protease formation are coordinately regulated. Both are produced only after culture growth ends, and they appear with essentially identical kinetics. In addition, strains which produce superior levels of cephamycin C form equally superior levels of the serine protease. Genetic evidence reveals that the syntheses of the antibiotic and serine proteases are associated with sporulation. Mutants which fail to produce aerial hyphae (bald mutants) also fail to synthesize the antibiotic and serine proteases."}
{"id": "PMID:464585", "title": "Modification of the colorimetric assay for chloramphenicol in the presence of bilirubin.", "content": "We recently found that bilirubin interferes with the colorimetric chloramphenicol assay of Kakemi et al. (K. T. Kakemi, T. Arita, and S. Ohashi, Yakugaku Zasshi 82:342-345, 1962). Levels of serum bilirubin alone (4 to 6 mg/dl) resulted in apparent concentrations of chloramphenicol which appear to be in the therapeutic range. Concentrations of serum bilirubin greater than 8 mg/dl resulted in levels of apparent chloramphenicol associated with toxicity (>50 mug/ml). Small amounts of activated charcoal added to the isoamyl acetate extraction step of the assay eliminated this interference by bilirubin.", "contents": "Modification of the colorimetric assay for chloramphenicol in the presence of bilirubin. We recently found that bilirubin interferes with the colorimetric chloramphenicol assay of Kakemi et al. (K. T. Kakemi, T. Arita, and S. Ohashi, Yakugaku Zasshi 82:342-345, 1962). Levels of serum bilirubin alone (4 to 6 mg/dl) resulted in apparent concentrations of chloramphenicol which appear to be in the therapeutic range. Concentrations of serum bilirubin greater than 8 mg/dl resulted in levels of apparent chloramphenicol associated with toxicity (>50 mug/ml). Small amounts of activated charcoal added to the isoamyl acetate extraction step of the assay eliminated this interference by bilirubin."}
{"id": "PMID:464586", "title": "Effect of pyran on latency after herpes simplex virus infections.", "content": "The immunomodulator pyran protected mice against both herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infections. In infections of the lip with HSV-1, prophylactic administration of pyran reduced the severity of the herpetic lesions and enhanced their resolution, but did not decrease the high incidence of development of latent HSV-1 infection of the trigeminal ganglia. In vaginal infections with HSV-2, prophylactic administration of pyran either systemically or locally reduced mortality, reduced the incidence of mice with vaginal HSV-2 infection, and did not alter the low incidence of latent infection of the spinal dorsal root ganglia. Pyran treatment before systemic herpetic infection after intravenous inoculation of HSV-2 also reduced mortality and virus replication, as evidenced by a decreased antibody response in the survivors, and it either reduced latent infection in the spinal dorsal root ganglia or did not predispose mice to latent infection. Treatment with the immunomodulator appeared to inhibit or reduce HSV infection early in viral pathogenesis in all three model systems, producing protection from clinical disease and resulting in less virus to induce a systemic antibody response, with either a reduction in latent virus infection or no enhancement of development of latency. In all of the HSV models, the development of latent herpetic infection was closely correlated with sufficient virus replication early in the infection to induce a systemic neutralizing-antibody response.", "contents": "Effect of pyran on latency after herpes simplex virus infections. The immunomodulator pyran protected mice against both herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infections. In infections of the lip with HSV-1, prophylactic administration of pyran reduced the severity of the herpetic lesions and enhanced their resolution, but did not decrease the high incidence of development of latent HSV-1 infection of the trigeminal ganglia. In vaginal infections with HSV-2, prophylactic administration of pyran either systemically or locally reduced mortality, reduced the incidence of mice with vaginal HSV-2 infection, and did not alter the low incidence of latent infection of the spinal dorsal root ganglia. Pyran treatment before systemic herpetic infection after intravenous inoculation of HSV-2 also reduced mortality and virus replication, as evidenced by a decreased antibody response in the survivors, and it either reduced latent infection in the spinal dorsal root ganglia or did not predispose mice to latent infection. Treatment with the immunomodulator appeared to inhibit or reduce HSV infection early in viral pathogenesis in all three model systems, producing protection from clinical disease and resulting in less virus to induce a systemic antibody response, with either a reduction in latent virus infection or no enhancement of development of latency. In all of the HSV models, the development of latent herpetic infection was closely correlated with sufficient virus replication early in the infection to induce a systemic neutralizing-antibody response."}
{"id": "PMID:464587", "title": "Effect of acycloguanosine treatment of acute and latent herpes simplex infections in mice.", "content": "Systemic treatment of mice with the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine [aciclovir]) was found to be highly effective against acute type 1 herpes simplex virus infection of the pinna. The drug ablated clinical signs and reduced virus replication both in tissue local to the inoculation site and within the nervous system. Provided that moderate-sized virus inocula were used, acycloguanosine treatment reduced or prevented the establishment of a latent infection in the dorsal root ganglia relating to the sensory nerve supply of the ear. However, although it aborted artificially produced infections in dorsal root ganglia, acycloguanosine was found not to be effective against the latent infection once established. This finding strongly indicated that latent herpes simplex virus in mice can exist in a nonreplicating form.", "contents": "Effect of acycloguanosine treatment of acute and latent herpes simplex infections in mice. Systemic treatment of mice with the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine [aciclovir]) was found to be highly effective against acute type 1 herpes simplex virus infection of the pinna. The drug ablated clinical signs and reduced virus replication both in tissue local to the inoculation site and within the nervous system. Provided that moderate-sized virus inocula were used, acycloguanosine treatment reduced or prevented the establishment of a latent infection in the dorsal root ganglia relating to the sensory nerve supply of the ear. However, although it aborted artificially produced infections in dorsal root ganglia, acycloguanosine was found not to be effective against the latent infection once established. This finding strongly indicated that latent herpes simplex virus in mice can exist in a nonreplicating form."}
{"id": "PMID:464588", "title": "Comparison between agar and broth minimum inhibitory concentrations of cefamandole, Cefoxitin, and cefuroxime.", "content": "The in vitro susceptibility to cefamandole, cefoxitin, and cefuroxime of clinical isolates of Streptococcus faecalis, Klebsiella, and indole-positive Proteus was assayed using minimum inhibitory concentration (MIC) determinations in broth and on solid media. It could be demonstrated that the agar dilution MICs obtained when S. faecalis was tested against cefuroxime and when indole-positive Proteus strains were tested against cefamandole tended to be considerably lower than those obtained with the broth dilution technique. The Klebsiella strains tested did not show any major differences with regard to MICs in broth or on solid media. Using an animal experimental infection model it could be demonstrated that with indole-positive Proteus the higher broth MIC correlated better to the observed data than the lower agar MIC when a beta-lactamase-producing strain was tested. The data obtained indicated that the beta-lactamase of the indole-positive Proteus strain was inducible. The results of the study gave evidence for a risk of false susceptibility of some bacterial species against cefamandole when agar techniques, e.g. paper disk diffusion, are used. For cefuroxime, the same phenomenon can be expected with S. faecalis and to some extent with indole-positive Proteus. In this study, cefoxitin seemed considerably less affected by the technique used for susceptibility testing.", "contents": "Comparison between agar and broth minimum inhibitory concentrations of cefamandole, Cefoxitin, and cefuroxime. The in vitro susceptibility to cefamandole, cefoxitin, and cefuroxime of clinical isolates of Streptococcus faecalis, Klebsiella, and indole-positive Proteus was assayed using minimum inhibitory concentration (MIC) determinations in broth and on solid media. It could be demonstrated that the agar dilution MICs obtained when S. faecalis was tested against cefuroxime and when indole-positive Proteus strains were tested against cefamandole tended to be considerably lower than those obtained with the broth dilution technique. The Klebsiella strains tested did not show any major differences with regard to MICs in broth or on solid media. Using an animal experimental infection model it could be demonstrated that with indole-positive Proteus the higher broth MIC correlated better to the observed data than the lower agar MIC when a beta-lactamase-producing strain was tested. The data obtained indicated that the beta-lactamase of the indole-positive Proteus strain was inducible. The results of the study gave evidence for a risk of false susceptibility of some bacterial species against cefamandole when agar techniques, e.g. paper disk diffusion, are used. For cefuroxime, the same phenomenon can be expected with S. faecalis and to some extent with indole-positive Proteus. In this study, cefoxitin seemed considerably less affected by the technique used for susceptibility testing."}
{"id": "PMID:464589", "title": "Bactericidal effect of combinations of antimicrobial drugs and antineoplastic antibiotics against Staphylococcus aureus.", "content": "Six antineoplastic antibiotics were tested against ten strains of Staphylococcus aureus. Four showed bacteriostatic and/or bactericidal activity against each of the ten strains, and two were only bacteriostatic for seven and nine strains, respectively. Using the cellophane transfer technique, combinations of these antineoplastic antibiotics with 16 antibacterial drugs were screened for combined bactericidal activity. Synergism or antagonism was demonstrated in about one-third of the combinations. Checkerboard titrations and killing curves confirmed these findings and indicated that the effective concentrations of the antibacterial agents were similar to those attainable in the serum after therapeutic doses of these drugs. Although the pharmacokinetics of the six antineoplastic antibiotics in humans are not fully known, at least one of them has a peak serum level corresponding to those values at which a bactericidal effect was produced in vitro.", "contents": "Bactericidal effect of combinations of antimicrobial drugs and antineoplastic antibiotics against Staphylococcus aureus. Six antineoplastic antibiotics were tested against ten strains of Staphylococcus aureus. Four showed bacteriostatic and/or bactericidal activity against each of the ten strains, and two were only bacteriostatic for seven and nine strains, respectively. Using the cellophane transfer technique, combinations of these antineoplastic antibiotics with 16 antibacterial drugs were screened for combined bactericidal activity. Synergism or antagonism was demonstrated in about one-third of the combinations. Checkerboard titrations and killing curves confirmed these findings and indicated that the effective concentrations of the antibacterial agents were similar to those attainable in the serum after therapeutic doses of these drugs. Although the pharmacokinetics of the six antineoplastic antibiotics in humans are not fully known, at least one of them has a peak serum level corresponding to those values at which a bactericidal effect was produced in vitro."}
{"id": "PMID:464590", "title": "Pharmacokinetic determinants of penicillin cure of gonococcal urethritis.", "content": "In a 1964 study of the pharmacokinetic determinants of penicillin cure of gonococcal urethritis, 45 male prisoner volunteers were experimentally infected with strains of Neisseria gonorrhoeae having known in vitro penicillin susceptibility. After developing urethritis, subjects received intramuscular penicillin G and had serum samples obtained serially to determine penicillin concentration. Using a multiple regression technique, we studied patient-associated parameters and parameters of the serum penicillin curves to determine the best predictors of treatment results. Cure was best predicted by the time the serum penicillin concentration remained above three to four times the penicillin minimum inhibitory concentration of the infecting strain (probability of correct classification, >0.80). Those cured had serum penicillin concentrations which remained in this range for means of 7 to 10 h. Our findings confirm principles of antimicrobial therapy derived from animal models and may have application in studying therapy of gonorrhea and other infectious diseases.", "contents": "Pharmacokinetic determinants of penicillin cure of gonococcal urethritis. In a 1964 study of the pharmacokinetic determinants of penicillin cure of gonococcal urethritis, 45 male prisoner volunteers were experimentally infected with strains of Neisseria gonorrhoeae having known in vitro penicillin susceptibility. After developing urethritis, subjects received intramuscular penicillin G and had serum samples obtained serially to determine penicillin concentration. Using a multiple regression technique, we studied patient-associated parameters and parameters of the serum penicillin curves to determine the best predictors of treatment results. Cure was best predicted by the time the serum penicillin concentration remained above three to four times the penicillin minimum inhibitory concentration of the infecting strain (probability of correct classification, >0.80). Those cured had serum penicillin concentrations which remained in this range for means of 7 to 10 h. Our findings confirm principles of antimicrobial therapy derived from animal models and may have application in studying therapy of gonorrhea and other infectious diseases."}
{"id": "PMID:464591", "title": "Effect of time and concentration upon interaction between gentamicin, tobramycin, Netilmicin, or amikacin and carbenicillin or ticarcillin.", "content": "An aminoglycoside antibiotic and carbenicillin or ticarcillin are widely used in the treatment of patients with gram-negative bacillus infections. This study evaluated the effect of time upon in vitro interaction between mixtures of four aminoglycosides at two concentrations with carbenicillin or ticarcillin at four concentrations. By linear regression analysis, the inactivation of each aminoglycoside was shown to be directly proportional to the concentration of carbenicillin (P < 0.001). Inactivation was significantly (P < 0.01) greater for gentamicin and tobramycin than for amikacin or netilmicin at all carbenicillin concentrations. At carbenicillin concentrations of 300 and 600 mug/ml, significantly (P < 0.005) less inactivation of amikacin occurred when compared to netilmicin. Ticarcillin produced a significant (P < 0.025) inactivation of gentamicin and tobramycin, with inactivation being directly proportional to ticarcillin concentration. No inactivation of amikacin or netilmicin activity occurred unless the ticarcillin concentration was 600 mug/ml. No significant change in aminoglycoside activity occurred when stored with ticarcillin or carbenicillin at concentrations ranging from 100 to 600 mug/ml at -70 degrees C for 56 days. When an aminoglycoside and carbenicillin or ticarcillin are indicated in patients with renal failure, this study supports the use of ticarcillin with either amikacin or netilmicin.", "contents": "Effect of time and concentration upon interaction between gentamicin, tobramycin, Netilmicin, or amikacin and carbenicillin or ticarcillin. An aminoglycoside antibiotic and carbenicillin or ticarcillin are widely used in the treatment of patients with gram-negative bacillus infections. This study evaluated the effect of time upon in vitro interaction between mixtures of four aminoglycosides at two concentrations with carbenicillin or ticarcillin at four concentrations. By linear regression analysis, the inactivation of each aminoglycoside was shown to be directly proportional to the concentration of carbenicillin (P < 0.001). Inactivation was significantly (P < 0.01) greater for gentamicin and tobramycin than for amikacin or netilmicin at all carbenicillin concentrations. At carbenicillin concentrations of 300 and 600 mug/ml, significantly (P < 0.005) less inactivation of amikacin occurred when compared to netilmicin. Ticarcillin produced a significant (P < 0.025) inactivation of gentamicin and tobramycin, with inactivation being directly proportional to ticarcillin concentration. No inactivation of amikacin or netilmicin activity occurred unless the ticarcillin concentration was 600 mug/ml. No significant change in aminoglycoside activity occurred when stored with ticarcillin or carbenicillin at concentrations ranging from 100 to 600 mug/ml at -70 degrees C for 56 days. When an aminoglycoside and carbenicillin or ticarcillin are indicated in patients with renal failure, this study supports the use of ticarcillin with either amikacin or netilmicin."}
{"id": "PMID:464592", "title": "Antifungal activity of tioconazole (UK-20,349), a new imidazole derivative.", "content": "Tioconazole (UK-20,349), a new antifungal imidazole derivative, was compared with miconazole for activity in vitro against Candida spp., Torulopsis glabrata, Cryptococcus neoformans, Aspergillus spp., and dermatophyte fungi (Trichophyton spp. and Microsporum spp.). Tioconazole was more active than miconazole against all the fungal species examined except Aspergillus, against which both agents showed similar activity. Both tioconazole and miconazole inhibited the growth of all fungi examined at concentrations well below their quoted minimum inhibitory concentrations. Their activity against fungi in vivo was investigated in mice infected systemically with Candida albicans. Both agents significantly reduced the numbers of viable Candida cells recoverable from the kidneys of infected animals, with tioconazole producing a generally more marked reduction. After administration of a single oral dose (25 mg/kg) to beagle dogs or white mice, higher and more sustained circulating levels of bioactive drug were detectable of tioconazole than of miconazole. These observations suggest that tioconazole may have potential in the treatment of both superficial and systemic mycoses in humans.", "contents": "Antifungal activity of tioconazole (UK-20,349), a new imidazole derivative. Tioconazole (UK-20,349), a new antifungal imidazole derivative, was compared with miconazole for activity in vitro against Candida spp., Torulopsis glabrata, Cryptococcus neoformans, Aspergillus spp., and dermatophyte fungi (Trichophyton spp. and Microsporum spp.). Tioconazole was more active than miconazole against all the fungal species examined except Aspergillus, against which both agents showed similar activity. Both tioconazole and miconazole inhibited the growth of all fungi examined at concentrations well below their quoted minimum inhibitory concentrations. Their activity against fungi in vivo was investigated in mice infected systemically with Candida albicans. Both agents significantly reduced the numbers of viable Candida cells recoverable from the kidneys of infected animals, with tioconazole producing a generally more marked reduction. After administration of a single oral dose (25 mg/kg) to beagle dogs or white mice, higher and more sustained circulating levels of bioactive drug were detectable of tioconazole than of miconazole. These observations suggest that tioconazole may have potential in the treatment of both superficial and systemic mycoses in humans."}
{"id": "PMID:464593", "title": "Effects of miconazole and dodecylimidazole on sterol biosynthesis in Ustilago maydis.", "content": "Miconazole at minimal fungitoxic concentrations inhibits ergosterol biosynthesis in sporidia of Ustilago maydis by interference with sterol C14 demethylation. The action is analogous to that of the fungicides triarimol and fenarimol. The fungicide 1-dodecylimidazole at low concentrations (0.1 to 0.25 mug/ml) inhibits sterol C14 demethylation; however, at higher concentrations (1.0 mug/ml or greater) it also inhibits 2,3-oxidosqualene cyclization and subsequent transmethylation. It is postulated that this diversity of effects of 1-dodecylimidazole results from binding of the inhibitor to sterol carrier protein(s).", "contents": "Effects of miconazole and dodecylimidazole on sterol biosynthesis in Ustilago maydis. Miconazole at minimal fungitoxic concentrations inhibits ergosterol biosynthesis in sporidia of Ustilago maydis by interference with sterol C14 demethylation. The action is analogous to that of the fungicides triarimol and fenarimol. The fungicide 1-dodecylimidazole at low concentrations (0.1 to 0.25 mug/ml) inhibits sterol C14 demethylation; however, at higher concentrations (1.0 mug/ml or greater) it also inhibits 2,3-oxidosqualene cyclization and subsequent transmethylation. It is postulated that this diversity of effects of 1-dodecylimidazole results from binding of the inhibitor to sterol carrier protein(s)."}
{"id": "PMID:464594", "title": "Pharmacokinetics of intravenous amoxicillin in pediatric patients.", "content": "Pharmacokinetic parameters were obtained from 14 infants and children receiving intravenous amoxicillin. Peak serum values increased proportionally to the increase in dose; the serum half-life was similar in the three dose groups studied.", "contents": "Pharmacokinetics of intravenous amoxicillin in pediatric patients. Pharmacokinetic parameters were obtained from 14 infants and children receiving intravenous amoxicillin. Peak serum values increased proportionally to the increase in dose; the serum half-life was similar in the three dose groups studied."}
{"id": "PMID:464595", "title": "Skin potential and EMG changes induced by cutaneous electrical stimulation. I. Normal man in arousing and non-arousing environment.", "content": "Skin potential and EMG responses induced in normal man by electrical stimuli applied to the skin were recorded in the four limbs in order to study somato-sympathetic and somato-motor reflexes. Different patterns of responses were observed in different conditions: alarm, habituation, sensitization and arousal. During alarm, sensitization and arousal, the responses were present in the four limbs; during habituation, the responses were only present in the stimulated and in the contralateral limb. Three sensory thresholds to cutaneous electrical stimulation were identified in habituated subjects: tactile, tingling and pain. Cutaneous and EMG responses appeared at tingling threshold. A relationship between skin potential level and skin potential response was observed.", "contents": "Skin potential and EMG changes induced by cutaneous electrical stimulation. I. Normal man in arousing and non-arousing environment. Skin potential and EMG responses induced in normal man by electrical stimuli applied to the skin were recorded in the four limbs in order to study somato-sympathetic and somato-motor reflexes. Different patterns of responses were observed in different conditions: alarm, habituation, sensitization and arousal. During alarm, sensitization and arousal, the responses were present in the four limbs; during habituation, the responses were only present in the stimulated and in the contralateral limb. Three sensory thresholds to cutaneous electrical stimulation were identified in habituated subjects: tactile, tingling and pain. Cutaneous and EMG responses appeared at tingling threshold. A relationship between skin potential level and skin potential response was observed."}
{"id": "PMID:464596", "title": "Skin potential and EMG changes induced by cutaneous electrical stimulation. II. Subjects with reflex sympathetic dystrophies.", "content": "In a group of patients suffering from reflex sympathetic dystrophies, the skin potential and EMG responses induced by electrical stimuli applied to the skin were recorded in the four limbs in order to study somato-sympathetic and somato-motor reflexes. In most patients, the amplitude, delay and shape of the cutaneous responses as well as the pattern of the EMG responses were different from those observed in normal subjects. In particular, it was possible to correlate the pattern of the cutaneous and muscular responses with the severity of the disease. The cutaneous sensory thresholds to electrical stimuli (tactile, tingling and pain threshold) showed different values in the dystrophic and in the contralateral limb. In all patients, a block of the sympathetic chain ipsilateral to the dystrophic limb was performed with local anesthetics. 1 h after the block, the cutaneous responses disappeared not only in the blocked limb but also in the contralateral limb. 48 h after the block, muscular and cutaneous responses as well as sensory thresholds showed a pattern similar to that observed in normal subjects. These findings show that the sympathetic block provides a resetting of the sensory thresholds and reflexes.", "contents": "Skin potential and EMG changes induced by cutaneous electrical stimulation. II. Subjects with reflex sympathetic dystrophies. In a group of patients suffering from reflex sympathetic dystrophies, the skin potential and EMG responses induced by electrical stimuli applied to the skin were recorded in the four limbs in order to study somato-sympathetic and somato-motor reflexes. In most patients, the amplitude, delay and shape of the cutaneous responses as well as the pattern of the EMG responses were different from those observed in normal subjects. In particular, it was possible to correlate the pattern of the cutaneous and muscular responses with the severity of the disease. The cutaneous sensory thresholds to electrical stimuli (tactile, tingling and pain threshold) showed different values in the dystrophic and in the contralateral limb. In all patients, a block of the sympathetic chain ipsilateral to the dystrophic limb was performed with local anesthetics. 1 h after the block, the cutaneous responses disappeared not only in the blocked limb but also in the contralateral limb. 48 h after the block, muscular and cutaneous responses as well as sensory thresholds showed a pattern similar to that observed in normal subjects. These findings show that the sympathetic block provides a resetting of the sensory thresholds and reflexes."}
{"id": "PMID:464597", "title": "Influence of temporal lobe lesions on photic-evoked responses in occipital visual areas.", "content": "In 20 patients with temporal lobe lesions and 10 controls, the averaged photic-evoked responses (APERs) and their dispersion pattern (DP) were investigated in inion-vertex-lead and bilaterally in inion-parietal leads (I-P3 and I-P4). The patients with temporal lobe lesions, regardless of lesion location in temporal areas, displayed either absence of APER (the whole APER or only the initial components) or a latency increase without amplitude changes. The DP was generally abnormal. The role of the temporal lobe in the organization of APER in visual areas is discussed.", "contents": "Influence of temporal lobe lesions on photic-evoked responses in occipital visual areas. In 20 patients with temporal lobe lesions and 10 controls, the averaged photic-evoked responses (APERs) and their dispersion pattern (DP) were investigated in inion-vertex-lead and bilaterally in inion-parietal leads (I-P3 and I-P4). The patients with temporal lobe lesions, regardless of lesion location in temporal areas, displayed either absence of APER (the whole APER or only the initial components) or a latency increase without amplitude changes. The DP was generally abnormal. The role of the temporal lobe in the organization of APER in visual areas is discussed."}
{"id": "PMID:464598", "title": "Relapsing neuropathy, cerebral atrophy and folate deficiency. A close association.", "content": "The case of a 48-year-old woman with a relapsing radial palsy which followed diarrheic episodes is reported. She had low folate concentrations in serum and cerebrospinal fluid, as well as low and borderline values of cerebrospinal fluid and erythrocyte folates. She had chronic gastrointestinal disease with edema of jejunal mucosa. The patient had some degree of cerebral atrophy. The effect of folate therapy on the neuropathy was spectacular. A beneficial effect of the therapy has been observed on both EEG tracings and neuropsychological performances after 6 months of treatment.", "contents": "Relapsing neuropathy, cerebral atrophy and folate deficiency. A close association. The case of a 48-year-old woman with a relapsing radial palsy which followed diarrheic episodes is reported. She had low folate concentrations in serum and cerebrospinal fluid, as well as low and borderline values of cerebrospinal fluid and erythrocyte folates. She had chronic gastrointestinal disease with edema of jejunal mucosa. The patient had some degree of cerebral atrophy. The effect of folate therapy on the neuropathy was spectacular. A beneficial effect of the therapy has been observed on both EEG tracings and neuropsychological performances after 6 months of treatment."}
{"id": "PMID:464623", "title": "Lowered cutaneous and urinary levels of polyamines with clinical improvement in treated psoriasis.", "content": "Polyamine metabolism is important in cell proliferation and may play a role in the epidermal cell hyperproliferation of psoriasis. We have determined changes in polyamine levels in skin and urine accompanying clinical improvement in psoriasis following topical therapy. Nine hospitalized patients were examined at the beginning and end of their courses of treatment. Skin biopsy specimens and portions of 24-hour urine collections were analyzed for polyamines with a modified automatic amino acid analyzer. Treatment resulted in lower cutaneous levels of putrescine (by 50%, P less than .05), spermidine (by 24%, P less than .05), and spermine (by 35%, P less than .005), and lower urinary levels of spermidine (by 20%, P less than .025) and spermine (by 35%, P less than .025). These results suggest that in psoriasis, the skin significantly contributes to the levels of spermidine and spermine in systemic fluids. Topical therapy may reduce epidermal cell proliferation in psoriasis by lowering polyamine levels.", "contents": "Lowered cutaneous and urinary levels of polyamines with clinical improvement in treated psoriasis. Polyamine metabolism is important in cell proliferation and may play a role in the epidermal cell hyperproliferation of psoriasis. We have determined changes in polyamine levels in skin and urine accompanying clinical improvement in psoriasis following topical therapy. Nine hospitalized patients were examined at the beginning and end of their courses of treatment. Skin biopsy specimens and portions of 24-hour urine collections were analyzed for polyamines with a modified automatic amino acid analyzer. Treatment resulted in lower cutaneous levels of putrescine (by 50%, P less than .05), spermidine (by 24%, P less than .05), and spermine (by 35%, P less than .005), and lower urinary levels of spermidine (by 20%, P less than .025) and spermine (by 35%, P less than .025). These results suggest that in psoriasis, the skin significantly contributes to the levels of spermidine and spermine in systemic fluids. Topical therapy may reduce epidermal cell proliferation in psoriasis by lowering polyamine levels."}
{"id": "PMID:464624", "title": "Cancer and scleroderma.", "content": "Of 2,141 patients with scleroderma who were seen at the Mayo Clinic, Rochester, Minn, between 1959 and 1975, 78 had 87 internal malignancies. The relative frequency of the types of cancers was similar to that for the general population. The increased numbers of patients with breast and uterine carcinomas were consistent with the female preponderance in scleroderma. Contrary to previous reports, carcinoma of the lung was not the most frequent malignancy associated with scleroderma. The lymphoma-leukemia malignancies were second only to breast carcinoma in frequency, comprising about 17% of the malignancies. Both conditions developed within a three-year period in 68% (45/66) of our patients affected with systemic scleroderma, and this subgroup comprised a high mortality group. This study is not an epidemiologic one in the strictest sense, but it is a review of the experience of the Mayo Clinic with patients who have had both scleroderma and an internal malignancy.", "contents": "Cancer and scleroderma. Of 2,141 patients with scleroderma who were seen at the Mayo Clinic, Rochester, Minn, between 1959 and 1975, 78 had 87 internal malignancies. The relative frequency of the types of cancers was similar to that for the general population. The increased numbers of patients with breast and uterine carcinomas were consistent with the female preponderance in scleroderma. Contrary to previous reports, carcinoma of the lung was not the most frequent malignancy associated with scleroderma. The lymphoma-leukemia malignancies were second only to breast carcinoma in frequency, comprising about 17% of the malignancies. Both conditions developed within a three-year period in 68% (45/66) of our patients affected with systemic scleroderma, and this subgroup comprised a high mortality group. This study is not an epidemiologic one in the strictest sense, but it is a review of the experience of the Mayo Clinic with patients who have had both scleroderma and an internal malignancy."}
{"id": "PMID:464625", "title": "Lymphangiosarcoma arising from lymphangioma circumscriptum.", "content": "A lymphangiosarcoma arose at the site of a preexisting lymphangioma circumscriptum on the skin of the anterior part of the abdominal wall. To our knowledge, this is only the second such case to be reported, and in both patients, the preexisting lymphangioma circumscriptum had been exposed to substantial x-ray therapy. Since it is possible that x-irradiation may play a role in the development of this unusual malignant neoplasm, it seems advisable that lymphangioma circumscriptum not be exposed to substantial amounts of such radiation, if feasible.", "contents": "Lymphangiosarcoma arising from lymphangioma circumscriptum. A lymphangiosarcoma arose at the site of a preexisting lymphangioma circumscriptum on the skin of the anterior part of the abdominal wall. To our knowledge, this is only the second such case to be reported, and in both patients, the preexisting lymphangioma circumscriptum had been exposed to substantial x-ray therapy. Since it is possible that x-irradiation may play a role in the development of this unusual malignant neoplasm, it seems advisable that lymphangioma circumscriptum not be exposed to substantial amounts of such radiation, if feasible."}
{"id": "PMID:464626", "title": "Cutaneous and venous complications of pentazocine abuse.", "content": "A 55-year-old man with a two-year history of intramuscular pentazocine abuse had classical cutaneous findings as well as secondary venous thrombosis, which, to our knowledge, is previously undescribed. Histologic evaluation, including direct immunofluorescent microscopy, did not establish the mechanism of tissue destruction.", "contents": "Cutaneous and venous complications of pentazocine abuse. A 55-year-old man with a two-year history of intramuscular pentazocine abuse had classical cutaneous findings as well as secondary venous thrombosis, which, to our knowledge, is previously undescribed. Histologic evaluation, including direct immunofluorescent microscopy, did not establish the mechanism of tissue destruction."}
{"id": "PMID:464627", "title": "Folded skin associated with underlying nevus lipomatosus.", "content": "A mentally retarded female infant had abnormalities of the feet, hemiplegia, and an underlying nevus lipomatosus. To our knowledge, this represents the second reported case of marked folding of the skin; with an underlying nevus lipomatosus; this case demonstrated an association of this cutaneous disorder with multiple defects, including chromosomal abnormalities, which have not been previously reported.", "contents": "Folded skin associated with underlying nevus lipomatosus. A mentally retarded female infant had abnormalities of the feet, hemiplegia, and an underlying nevus lipomatosus. To our knowledge, this represents the second reported case of marked folding of the skin; with an underlying nevus lipomatosus; this case demonstrated an association of this cutaneous disorder with multiple defects, including chromosomal abnormalities, which have not been previously reported."}
{"id": "PMID:464628", "title": "Relapsing polychondritis associated with cutaneous vasculitis.", "content": "A patient with relapsing polychondritis associated with cutaneous vasculitis is described in detail. Clinically, a systemic chondritis complicated by conjunctivitis, iritis, otitis media, tracheal stenosis, sinusitis, and cutaneous vasculitis were observed in this case. Corticosteroids have been effective in controlling the clinical symptoms as well as the vasculitis.", "contents": "Relapsing polychondritis associated with cutaneous vasculitis. A patient with relapsing polychondritis associated with cutaneous vasculitis is described in detail. Clinically, a systemic chondritis complicated by conjunctivitis, iritis, otitis media, tracheal stenosis, sinusitis, and cutaneous vasculitis were observed in this case. Corticosteroids have been effective in controlling the clinical symptoms as well as the vasculitis."}
{"id": "PMID:464629", "title": "Localized pruritus-notalgia paresthetica.", "content": "Notalgia paresthetica, possibly an isolated sensory neuropathy involving the posterior primary rami of thoracic nerves T2 through T6, and appearing as pruritus of the back, is apt to be encountered by both dermatologists and neurologists. Two cases illustrate this disorder.", "contents": "Localized pruritus-notalgia paresthetica. Notalgia paresthetica, possibly an isolated sensory neuropathy involving the posterior primary rami of thoracic nerves T2 through T6, and appearing as pruritus of the back, is apt to be encountered by both dermatologists and neurologists. Two cases illustrate this disorder."}
{"id": "PMID:464630", "title": "Primary cutaneous cryptococcosis.", "content": "Cryptococcal infections in immuno-compromised patients are of major importance. Current opinion holds that cutaneous cryptococcal infections in these patients indicate disseminated disease. In our renal transplant patient with primary cutaneous cryptococcosis, intravenously administered antifungal chemotherapy resulted in resolution of the disease and appears to have prevented disseminated infection.", "contents": "Primary cutaneous cryptococcosis. Cryptococcal infections in immuno-compromised patients are of major importance. Current opinion holds that cutaneous cryptococcal infections in these patients indicate disseminated disease. In our renal transplant patient with primary cutaneous cryptococcosis, intravenously administered antifungal chemotherapy resulted in resolution of the disease and appears to have prevented disseminated infection."}
{"id": "PMID:464631", "title": "Photosensitivity reaction to dibucaine. Case report and experimental induction.", "content": "In a 13-year-old girl, photoallergic dermatitis was caused by dibucaine hydrochloride, which was used as a local anesthetic in the treatment of her dental caries. The action spectrum for the photosensitivity reaction was in the long-wave ultraviolet (UVA) range. Spectrophotometrically, dibucaine absorbed UVA up to 380 nm, and in the vitro irradiated chemical showed an altered absorption spectrum. The agent is fluorescent under UVA light. The allergic photocontact sensitization could be experimentally induced in guinea pigs. The chemical properties and high sensitization rate of experimental induction suggested that dibucaine may be a potent photosensitizing chemical. Dibucaine should be included in routine photopatch test materials.", "contents": "Photosensitivity reaction to dibucaine. Case report and experimental induction. In a 13-year-old girl, photoallergic dermatitis was caused by dibucaine hydrochloride, which was used as a local anesthetic in the treatment of her dental caries. The action spectrum for the photosensitivity reaction was in the long-wave ultraviolet (UVA) range. Spectrophotometrically, dibucaine absorbed UVA up to 380 nm, and in the vitro irradiated chemical showed an altered absorption spectrum. The agent is fluorescent under UVA light. The allergic photocontact sensitization could be experimentally induced in guinea pigs. The chemical properties and high sensitization rate of experimental induction suggested that dibucaine may be a potent photosensitizing chemical. Dibucaine should be included in routine photopatch test materials."}
{"id": "PMID:464632", "title": "Cutaneous phycomycosis. Report of three cases with identification of Rhizopus.", "content": "Three cases of pustular and ulcerative cutaneous phycomycosis developed postoperatively in orthopedic patients following exposure to a contaiminated surgical adhesive (Elastoplast). A review of the pertinent literature revealed no other similar series of reported cases. The epidemiologic investigation of these cases is discussed. This experience demonstrates that with appropriate exposure, normal saprophytes can invade the skin in immunocompetent patients, producing active and invasive lesions.", "contents": "Cutaneous phycomycosis. Report of three cases with identification of Rhizopus. Three cases of pustular and ulcerative cutaneous phycomycosis developed postoperatively in orthopedic patients following exposure to a contaiminated surgical adhesive (Elastoplast). A review of the pertinent literature revealed no other similar series of reported cases. The epidemiologic investigation of these cases is discussed. This experience demonstrates that with appropriate exposure, normal saprophytes can invade the skin in immunocompetent patients, producing active and invasive lesions."}
{"id": "PMID:464634", "title": "[Semen analysis under photochemotherapy (PUVA-therapy) (author's transl)].", "content": "In 9 male patients with psoriasis vulgaris a semen analysis before and during photochemotherapy with 8-methoxypsoralen and UVA (PUVA) was performed to rule out drug-induced toxic damage of spermatogenesis or impairment of fertility due to scrotal hyperthermia. Two hours after oral application of 40--60 mg 8-methoxypsoralen the patients had been irradiated in UVA high intensity treatment units. PUVA-treatments were performed four times weekly until total body clearing was achieved. For complete remission 13--26 (mean 20.5) PUVA-treatments were necessary. Corresponding total UVA-doses were 35.3--191.0 (mean 83.2) Joule/cm2. The investigated parameters total motility, progressive motility, spermatozoa density, total spermatozoa count, spermatozoa morphology, and seminal plasma fructose remained unchanged. Only the volume of the ejaculate showed a small decrease during 3 months of therapy. From this pilot study there is no evidence that PUVA-therapy leads to an impairment of fertility in male patients within their reproductive age.", "contents": "[Semen analysis under photochemotherapy (PUVA-therapy) (author's transl)]. In 9 male patients with psoriasis vulgaris a semen analysis before and during photochemotherapy with 8-methoxypsoralen and UVA (PUVA) was performed to rule out drug-induced toxic damage of spermatogenesis or impairment of fertility due to scrotal hyperthermia. Two hours after oral application of 40--60 mg 8-methoxypsoralen the patients had been irradiated in UVA high intensity treatment units. PUVA-treatments were performed four times weekly until total body clearing was achieved. For complete remission 13--26 (mean 20.5) PUVA-treatments were necessary. Corresponding total UVA-doses were 35.3--191.0 (mean 83.2) Joule/cm2. The investigated parameters total motility, progressive motility, spermatozoa density, total spermatozoa count, spermatozoa morphology, and seminal plasma fructose remained unchanged. Only the volume of the ejaculate showed a small decrease during 3 months of therapy. From this pilot study there is no evidence that PUVA-therapy leads to an impairment of fertility in male patients within their reproductive age."}
{"id": "PMID:464635", "title": "[Histology of the pigmented granular rankenneuroma (author's transl)].", "content": "We report on a case of a 40 years old female patient with a lentil-sized tumor of the left pinna present since early childhood. Histologic examination revealed pigmented granular \"rankenneuroma\". The lesion is unencapsulated but fairly well marginated in the dermis. It is composed of varying numbers of spindle-shaped or polyhedral cells arranged in intertwining and parallel bands separated by strands of collagen. The fascicles follow the preexisting nerve truncs. All layers of the dermis may be involved, but only rarely is the superficial subcutaneous tissue invaded. The fascicles of the tumor are generally constructed in a similar fashion. In the center one see argyrophile granular alterated unpigmented cells- Those elements are surrounded by mostly bipolar cells containing varying amounts of melanin. Silverstaining shows transition from spindle-shaped cells to granular degenerated elements. It is thought that the tumor most likely originates from the proliferation of the granular degenerated Schwann cells. The characteristic feature of tumor parenchyme is the association of the granular Schwann cells with cutaneous melanoblasts.", "contents": "[Histology of the pigmented granular rankenneuroma (author's transl)]. We report on a case of a 40 years old female patient with a lentil-sized tumor of the left pinna present since early childhood. Histologic examination revealed pigmented granular \"rankenneuroma\". The lesion is unencapsulated but fairly well marginated in the dermis. It is composed of varying numbers of spindle-shaped or polyhedral cells arranged in intertwining and parallel bands separated by strands of collagen. The fascicles follow the preexisting nerve truncs. All layers of the dermis may be involved, but only rarely is the superficial subcutaneous tissue invaded. The fascicles of the tumor are generally constructed in a similar fashion. In the center one see argyrophile granular alterated unpigmented cells- Those elements are surrounded by mostly bipolar cells containing varying amounts of melanin. Silverstaining shows transition from spindle-shaped cells to granular degenerated elements. It is thought that the tumor most likely originates from the proliferation of the granular degenerated Schwann cells. The characteristic feature of tumor parenchyme is the association of the granular Schwann cells with cutaneous melanoblasts."}
{"id": "PMID:464636", "title": "[Beneficial effect of oral retinoid in psoriatic hyperproliferation. Autoradiographic studies on involved and non involved skin (author's transl)].", "content": "In 16 patients with generalized psoriasis treated with oral retinoid (Ro 10-9359; 1 mg/kg body weight/(day) autoradiographic studies were performed before, and 24 h, 48 h, 1 week and 3 weeks after beginning of treatment on involved and non involved epidermis. During the first week no significant changes of all cellular parameters were found. However, after 3 weeks of continuing medication the increased 3H-thymidine labelling index was markedly lowered and the prolonged DNA synthesis time was shortened; whereas, the cell cycle time remained unaffected. The findings revealed that oral retinoid has a distinct influence on epidermal cell proliferation towards normalization in both involved and non involved epidermis in psoriasis. The time needed for this effect indicates that the drug does not directly interfere with the cell cycle. It seems reasonable, therefore, to combine oral retinoids as a basic adjuvant drug with other antipsoriatic techniques, such as retinoid + anthralin, retinoid + PUVA, retinoid + UVB.", "contents": "[Beneficial effect of oral retinoid in psoriatic hyperproliferation. Autoradiographic studies on involved and non involved skin (author's transl)]. In 16 patients with generalized psoriasis treated with oral retinoid (Ro 10-9359; 1 mg/kg body weight/(day) autoradiographic studies were performed before, and 24 h, 48 h, 1 week and 3 weeks after beginning of treatment on involved and non involved epidermis. During the first week no significant changes of all cellular parameters were found. However, after 3 weeks of continuing medication the increased 3H-thymidine labelling index was markedly lowered and the prolonged DNA synthesis time was shortened; whereas, the cell cycle time remained unaffected. The findings revealed that oral retinoid has a distinct influence on epidermal cell proliferation towards normalization in both involved and non involved epidermis in psoriasis. The time needed for this effect indicates that the drug does not directly interfere with the cell cycle. It seems reasonable, therefore, to combine oral retinoids as a basic adjuvant drug with other antipsoriatic techniques, such as retinoid + anthralin, retinoid + PUVA, retinoid + UVB."}
{"id": "PMID:464637", "title": "[Spindle-shaped cell blue naevus with lymph nodes \"metastases\" (author's transl)].", "content": "\"Metastases\" in lymph nodes are not only found from cellular blue naevi but also from their spindle-shaped cell fascicular type. These \"metastases\" are mostly situated in the fibrous capsule, very rarely in the parenchyma of the lymph nodes. Individually the cells resembled those of the cutaneous tumour. Their occurence in the peripheral sinuses should be verified. Epitheloid vessel-wall cells (glomus-type cells), sometimes pigmented and occasionally found in large cell aggregates, can be noted in the capsule of the lymph nodes. By several authors, these cells are considered to be benign naevus cells. The histopathogenesis and the clinical importance of the \"metastases\" from blue naevus are discussed.", "contents": "[Spindle-shaped cell blue naevus with lymph nodes \"metastases\" (author's transl)]. \"Metastases\" in lymph nodes are not only found from cellular blue naevi but also from their spindle-shaped cell fascicular type. These \"metastases\" are mostly situated in the fibrous capsule, very rarely in the parenchyma of the lymph nodes. Individually the cells resembled those of the cutaneous tumour. Their occurence in the peripheral sinuses should be verified. Epitheloid vessel-wall cells (glomus-type cells), sometimes pigmented and occasionally found in large cell aggregates, can be noted in the capsule of the lymph nodes. By several authors, these cells are considered to be benign naevus cells. The histopathogenesis and the clinical importance of the \"metastases\" from blue naevus are discussed."}
{"id": "PMID:464638", "title": "Proteinase binding and enhancing factor from human skin.", "content": "Fresh human skin extract made in salt solution after a prior buffer extraction was shown to enhance the hydrolysis of N-alpha-benzoyl-DL-arginine beta-naphthylamide (BANA) by trypsin. This trypsin enhancing effect was further shown to be both stabilizing and activating. After chromatography on Sephadex G-100, the trypsin binding factor was found in fractions of void volume. Protease binding took place in physiological and hypotonic but not in hypertonic NaCl-solutions (0.5 mol/l). The proteinase binding factor was further purified by trypsin-Sepharose 4 B affinity chromatography. It was found to bind also chymotrypsin and elastase and to be thermostable (100 degrees C for 20 min), precipitable at acidic pH (3.5), and by acetone and ammonium sulphate (60% saturation). The bound proteinases were found to preserve their hydrolytic activity towards protein substrates. Bound trypsin and chymotrypsin could completely be inhibited by soybean trypsin inhibitor. The binding factor did not react with anti-human-alfa2-macroglobulin antiserum from rabbit.", "contents": "Proteinase binding and enhancing factor from human skin. Fresh human skin extract made in salt solution after a prior buffer extraction was shown to enhance the hydrolysis of N-alpha-benzoyl-DL-arginine beta-naphthylamide (BANA) by trypsin. This trypsin enhancing effect was further shown to be both stabilizing and activating. After chromatography on Sephadex G-100, the trypsin binding factor was found in fractions of void volume. Protease binding took place in physiological and hypotonic but not in hypertonic NaCl-solutions (0.5 mol/l). The proteinase binding factor was further purified by trypsin-Sepharose 4 B affinity chromatography. It was found to bind also chymotrypsin and elastase and to be thermostable (100 degrees C for 20 min), precipitable at acidic pH (3.5), and by acetone and ammonium sulphate (60% saturation). The bound proteinases were found to preserve their hydrolytic activity towards protein substrates. Bound trypsin and chymotrypsin could completely be inhibited by soybean trypsin inhibitor. The binding factor did not react with anti-human-alfa2-macroglobulin antiserum from rabbit."}
{"id": "PMID:464639", "title": "Urinary histamine in severe atopic dermatitis.", "content": "Urinary histamine was studied in 11 patients with severe atopic dermatitis and 17 controls, as well as in seven atopics prior to and 6 months following hyposensitization. No statistically significant differences were found between the groups. Urinary histamine seems to be of no value as an indicator of disease activity in atopic dermatitis.", "contents": "Urinary histamine in severe atopic dermatitis. Urinary histamine was studied in 11 patients with severe atopic dermatitis and 17 controls, as well as in seven atopics prior to and 6 months following hyposensitization. No statistically significant differences were found between the groups. Urinary histamine seems to be of no value as an indicator of disease activity in atopic dermatitis."}
{"id": "PMID:464640", "title": "[Skin impedance and phoreographic index in psoriasis. Relationship with action kinetics of three treatments].", "content": "In a previous study, impedance measurements and phoreographic response were shown to quantify significant differences between involved and non-involved skin in psoriasis. The same techniques were used to objectivate the evolution on skin condition on patients treated with dioxyanthranol, difluprednate, and photochemotherapy associated with 8-methoxypsoralen. Each of these treatments was applied to four subjects. On patients treated with dioxyanthranol or difluprednate, impedance and phoreographic response return to \"normal\" values within 5--10 days and 1--2 days, respectively. It takes a longer time with photochemotherapy, and it is noteworthy that the treatment also alters the phoreographic response of non-involved skin. The changes observed in electrophysiological parameters are discussed in relation with recent ideas on pharmacology of antipsoriatic treatments and on dynamic properties of biological membranes. The results are in good correlation with clinical data and exemplify the usefulness of these methods in following the treatment kinetics.", "contents": "[Skin impedance and phoreographic index in psoriasis. Relationship with action kinetics of three treatments]. In a previous study, impedance measurements and phoreographic response were shown to quantify significant differences between involved and non-involved skin in psoriasis. The same techniques were used to objectivate the evolution on skin condition on patients treated with dioxyanthranol, difluprednate, and photochemotherapy associated with 8-methoxypsoralen. Each of these treatments was applied to four subjects. On patients treated with dioxyanthranol or difluprednate, impedance and phoreographic response return to \"normal\" values within 5--10 days and 1--2 days, respectively. It takes a longer time with photochemotherapy, and it is noteworthy that the treatment also alters the phoreographic response of non-involved skin. The changes observed in electrophysiological parameters are discussed in relation with recent ideas on pharmacology of antipsoriatic treatments and on dynamic properties of biological membranes. The results are in good correlation with clinical data and exemplify the usefulness of these methods in following the treatment kinetics."}
{"id": "PMID:464641", "title": "Influence of age and of desmotropic drugs on the step phenomenon observed in rat skin.", "content": "Comprehensive analysis of the mechanical properties of rat skin revealed the \"step phenomenon\". This particular observation was made after constant strain rate (analysis of stress strain curves) as well as after constant load (creep experiments). Relative low extensions or low loads were necessary to provoke the steps. In most cases two, sometimes three steps were observed. The step phenomenon was found mainly in skin strips punched out perpendicularly to the body axis. Probably some bonds in the fibrous network are broken giving way to additional elongation whereafter stronger links take over the stress. Since earlier studies demonstrated a pronounced influence of age and of desmotropic drugs on mechanical properties at ultimate load, e.g., tensile strength, ultimate modulus of elasticity, and ultimate strain, also the step phenomenon was studied under these conditions. In stress-strain experiments most of the steps were found at the ages of 2 and 4 months. Total stress loss and total work loss due to the steps were the highest at the age of 4 months. If, however, these values were calculated as percentage of ultimate values, the highest figures were found in young animals. Elongation gain due to the steps also showed a maximum at time of maturation, e.g., 4 months. Similar findings were achieved in creep experiments at medium load (200 g). After treatment with prednisolone acetate more steps and after treatment with D-penicillamine fewer steps were observed. In stress-strain experiments total stress loss and total work loss due to steps were more than twice as high than controls after prednisolone treatment and only one half after D-penicillamine. If calculated as percentage of ultimate stress or percentage of work input, these changes disappeared because of similar changes at ultimate load. However, elongation gain due to steps, which was not significantly influenced by prednisolone acetate but significantly decreased by D-penicillamine, showed the same changes when calculated as percentage of ultimate strain. Under all conditions the step phenomenon mainly influenced the extension parameters. The data presented here confirm earlier observations that mechanical properties at low loads or low and medium extensions show at least to some extent a different pattern under the influence of maturation and age and after treatment with desmotropic drugs compared to the mechanical parameters at ultimate load.", "contents": "Influence of age and of desmotropic drugs on the step phenomenon observed in rat skin. Comprehensive analysis of the mechanical properties of rat skin revealed the \"step phenomenon\". This particular observation was made after constant strain rate (analysis of stress strain curves) as well as after constant load (creep experiments). Relative low extensions or low loads were necessary to provoke the steps. In most cases two, sometimes three steps were observed. The step phenomenon was found mainly in skin strips punched out perpendicularly to the body axis. Probably some bonds in the fibrous network are broken giving way to additional elongation whereafter stronger links take over the stress. Since earlier studies demonstrated a pronounced influence of age and of desmotropic drugs on mechanical properties at ultimate load, e.g., tensile strength, ultimate modulus of elasticity, and ultimate strain, also the step phenomenon was studied under these conditions. In stress-strain experiments most of the steps were found at the ages of 2 and 4 months. Total stress loss and total work loss due to the steps were the highest at the age of 4 months. If, however, these values were calculated as percentage of ultimate values, the highest figures were found in young animals. Elongation gain due to the steps also showed a maximum at time of maturation, e.g., 4 months. Similar findings were achieved in creep experiments at medium load (200 g). After treatment with prednisolone acetate more steps and after treatment with D-penicillamine fewer steps were observed. In stress-strain experiments total stress loss and total work loss due to steps were more than twice as high than controls after prednisolone treatment and only one half after D-penicillamine. If calculated as percentage of ultimate stress or percentage of work input, these changes disappeared because of similar changes at ultimate load. However, elongation gain due to steps, which was not significantly influenced by prednisolone acetate but significantly decreased by D-penicillamine, showed the same changes when calculated as percentage of ultimate strain. Under all conditions the step phenomenon mainly influenced the extension parameters. The data presented here confirm earlier observations that mechanical properties at low loads or low and medium extensions show at least to some extent a different pattern under the influence of maturation and age and after treatment with desmotropic drugs compared to the mechanical parameters at ultimate load."}
{"id": "PMID:464643", "title": "Interaction of methotrexate and betamethasone with experimental phototoxic inflammation to PUVA (Psoralen and UVA) in mice.", "content": "The influence of methotrexate and of betamethasone on a following phototoxic reaction to 8-methoxypsoralen and long-wave ultraviolet light (PUVA) was studied in the mouse. High PUVA doses were not influenced by the two drugs tested. Both methotrexate and betamethasone tended to diminish the PUVA response when psoralen was given in a medium dose.", "contents": "Interaction of methotrexate and betamethasone with experimental phototoxic inflammation to PUVA (Psoralen and UVA) in mice. The influence of methotrexate and of betamethasone on a following phototoxic reaction to 8-methoxypsoralen and long-wave ultraviolet light (PUVA) was studied in the mouse. High PUVA doses were not influenced by the two drugs tested. Both methotrexate and betamethasone tended to diminish the PUVA response when psoralen was given in a medium dose."}
{"id": "PMID:464645", "title": "The sensitizing capacity of naturally occurring quinones. Experimental studies in guinea pigs. II. Benzoquinones.", "content": "Experimental studies on the sensitizing capacity of naturally occurring benzoquinones, isolated from plants and woods have been carried out in guinea pigs of the Pirbright white strain. Seven compounds were available: primin, three dalbergiones, mansonia quinone (mansonone A), 2,6-dimethoxybenzoquinone and rapanone. With five of these substances (primin, mansonone A, three dalbergiones) guinea pigs could be sensitized. Primin, the allergen of Primula obconica Hance (primrose) proved to be the most effective one of all quinones tested in this and the preceding studies. As a similar but weaker sensitizer R-3, 4-dimethoxydalbergione from Machaerium scleroxylon Tul. (Pao ferro, Caviuna vermelha) could be identified. The results obtained with mansonone A, a sesquiterpenoid quinone from Mansonia altissima A. Chev. demonstrate that even naturally occurring orthoquinones are capable of inducing contact allergy. Allergic cross reactions could be obtained between all chemically related mansonones A-F. The results are in good accordance with the view that the sensitizing capacity of naturally occurring quinones depends on the fundamental quinoid structure and the length, position and configuration of the aliphatic side-chain.", "contents": "The sensitizing capacity of naturally occurring quinones. Experimental studies in guinea pigs. II. Benzoquinones. Experimental studies on the sensitizing capacity of naturally occurring benzoquinones, isolated from plants and woods have been carried out in guinea pigs of the Pirbright white strain. Seven compounds were available: primin, three dalbergiones, mansonia quinone (mansonone A), 2,6-dimethoxybenzoquinone and rapanone. With five of these substances (primin, mansonone A, three dalbergiones) guinea pigs could be sensitized. Primin, the allergen of Primula obconica Hance (primrose) proved to be the most effective one of all quinones tested in this and the preceding studies. As a similar but weaker sensitizer R-3, 4-dimethoxydalbergione from Machaerium scleroxylon Tul. (Pao ferro, Caviuna vermelha) could be identified. The results obtained with mansonone A, a sesquiterpenoid quinone from Mansonia altissima A. Chev. demonstrate that even naturally occurring orthoquinones are capable of inducing contact allergy. Allergic cross reactions could be obtained between all chemically related mansonones A-F. The results are in good accordance with the view that the sensitizing capacity of naturally occurring quinones depends on the fundamental quinoid structure and the length, position and configuration of the aliphatic side-chain."}
{"id": "PMID:464647", "title": "Increased elongated and stabilized microtubules in psoriatic keratinocytes.", "content": "Microtubules (MT) occur in the keratinocytes as 20 nm broad tubular structures with an electron-lucent core surrounded by an electron-dense wall. The number and the length of the MT increased to the upper epidermal layers. In the normal keratinocytes the MT were relatively short (maximal length 2.2 microns) and slightly tortuous. Their outline was tenuous and irregular. Sometimes the MT were interrupted indicating a high fragility. In the upper layers of the psoriatic epidermis the MT reached a maximal length of 3.1 microns, showing a greater elongation than in the normal epidermis. The MT were stright and distinctly limited, most likely resulting from a higher grade of stabilization or polymerisation. By their interaction with the tonofilaments the MT may contribute to the stability of the cell shape, delaying the flattening of the psoriatic keratinocytes in the upper epidermal layers. Nowhere did the MT make direct contact with the cell membrane. At some places they did gain access to the cell membrane by short microfilaments.", "contents": "Increased elongated and stabilized microtubules in psoriatic keratinocytes. Microtubules (MT) occur in the keratinocytes as 20 nm broad tubular structures with an electron-lucent core surrounded by an electron-dense wall. The number and the length of the MT increased to the upper epidermal layers. In the normal keratinocytes the MT were relatively short (maximal length 2.2 microns) and slightly tortuous. Their outline was tenuous and irregular. Sometimes the MT were interrupted indicating a high fragility. In the upper layers of the psoriatic epidermis the MT reached a maximal length of 3.1 microns, showing a greater elongation than in the normal epidermis. The MT were stright and distinctly limited, most likely resulting from a higher grade of stabilization or polymerisation. By their interaction with the tonofilaments the MT may contribute to the stability of the cell shape, delaying the flattening of the psoriatic keratinocytes in the upper epidermal layers. Nowhere did the MT make direct contact with the cell membrane. At some places they did gain access to the cell membrane by short microfilaments."}
{"id": "PMID:464648", "title": "Monocytopoiesis in malignant melanoma: untreated, during immunotherapy and chemoimmunotherapy.", "content": "Monocytopoiesis and blood monocytes were investigated in patients with superficial spreading melanoma stages I and II. Monocyte production was moderately increased in 4 of 9 untreated patients. Postoperative prophylactic BCG-vaccination gave rise to increased proliferation activity in 3 of 4 patients with previously normal monocytopoiesis. However, monocyte production returned to normal between the 4th and 6th month of BCG immunotherapy. Monocytopoietic hyperproliferation did not occur if DTIC was administered simultaneously with BCG. These results indicate that BCG-vaccination increases monocytopoiesis during the first months of treatment only. This effect is abrogated by concomitant chemotherapy.", "contents": "Monocytopoiesis in malignant melanoma: untreated, during immunotherapy and chemoimmunotherapy. Monocytopoiesis and blood monocytes were investigated in patients with superficial spreading melanoma stages I and II. Monocyte production was moderately increased in 4 of 9 untreated patients. Postoperative prophylactic BCG-vaccination gave rise to increased proliferation activity in 3 of 4 patients with previously normal monocytopoiesis. However, monocyte production returned to normal between the 4th and 6th month of BCG immunotherapy. Monocytopoietic hyperproliferation did not occur if DTIC was administered simultaneously with BCG. These results indicate that BCG-vaccination increases monocytopoiesis during the first months of treatment only. This effect is abrogated by concomitant chemotherapy."}
{"id": "PMID:464649", "title": "The effect of oral zinc loading on the absorption of 65zinc in the rat.", "content": "Seven groups of 8 rats each were orally loaded with zinc, the daily dose varying from 1.8 to 58 mg, corresponding to about 3 to 100 times of their estimated daily intake of zinc. To record the absorption of zinc, the rats were given a single dose of 65Zn. The retention of the isotope was measured in a whole animal counter at regular intervals. The dose of 58 mg was obviously toxic, since half of the animals died within 5 days. The net absorption of zinc in the remaining experimental groups was found to vary from about 7% in the group receiving the smallest loading dose to 1.8% in the group receiving the highest dose. From the absorption values, as determined by extrapolation of semilog retention curves, the total amount of absorbed zinc was estimated. It was found to differ from about 170 micrograms to about 530 micrograms zinc daily, increasing three times as the loading dose was increased 16 times. This discrepancy suggests the existence of regulatory mechanisms of the absorption of zinc from the intestine.", "contents": "The effect of oral zinc loading on the absorption of 65zinc in the rat. Seven groups of 8 rats each were orally loaded with zinc, the daily dose varying from 1.8 to 58 mg, corresponding to about 3 to 100 times of their estimated daily intake of zinc. To record the absorption of zinc, the rats were given a single dose of 65Zn. The retention of the isotope was measured in a whole animal counter at regular intervals. The dose of 58 mg was obviously toxic, since half of the animals died within 5 days. The net absorption of zinc in the remaining experimental groups was found to vary from about 7% in the group receiving the smallest loading dose to 1.8% in the group receiving the highest dose. From the absorption values, as determined by extrapolation of semilog retention curves, the total amount of absorbed zinc was estimated. It was found to differ from about 170 micrograms to about 530 micrograms zinc daily, increasing three times as the loading dose was increased 16 times. This discrepancy suggests the existence of regulatory mechanisms of the absorption of zinc from the intestine."}
{"id": "PMID:464650", "title": "Therapy of psoriasis with retinoid plus PUVA: clinical and histologic data.", "content": "In a group of 40 patients suffering from wide-spread psoriasis oral administration of a retinoid (Ro 10-9359) was followed by PUVA therapy. The clearance rate was increased by 30% as compared to PUVA alone. Except for cheilitis no side effects were seen. Histological analysis in 20 patients before, during and after therapy revealed an intensification of psoriatic tissue changes after retinoid treatment. Loss of corneal layers, massive exoserosis, and neutrophil migration were prominent features. Mitotic counts were not increased by the pretreatment. The increased susceptibility of diseased skin to PUVA as produced by this drug appears to be based on several factors related to the tissue changes revealed by histology.", "contents": "Therapy of psoriasis with retinoid plus PUVA: clinical and histologic data. In a group of 40 patients suffering from wide-spread psoriasis oral administration of a retinoid (Ro 10-9359) was followed by PUVA therapy. The clearance rate was increased by 30% as compared to PUVA alone. Except for cheilitis no side effects were seen. Histological analysis in 20 patients before, during and after therapy revealed an intensification of psoriatic tissue changes after retinoid treatment. Loss of corneal layers, massive exoserosis, and neutrophil migration were prominent features. Mitotic counts were not increased by the pretreatment. The increased susceptibility of diseased skin to PUVA as produced by this drug appears to be based on several factors related to the tissue changes revealed by histology."}
{"id": "PMID:464651", "title": "[Fatty acid composition of lipids from the hair in psoriasis vulgaris (author's transl)].", "content": "The composition of fatty acids of hair lipids and the sum of hair and scalp lipids from subjects with Psoriasis vulgaris and a control group was determined by gas liquid chromatography. The analyses indicates that the percentage compositions of lauric acid was distinctly higher by Psoriasis vulgaris.", "contents": "[Fatty acid composition of lipids from the hair in psoriasis vulgaris (author's transl)]. The composition of fatty acids of hair lipids and the sum of hair and scalp lipids from subjects with Psoriasis vulgaris and a control group was determined by gas liquid chromatography. The analyses indicates that the percentage compositions of lauric acid was distinctly higher by Psoriasis vulgaris."}
{"id": "PMID:464652", "title": "HLA-antigens in systemic lupus erythematosus (SLE).", "content": "Histocompatibility antigens were determined in 27 patients with SLE and 283 normal blood donors. HLA-B8 was significantly increased in patients with SLE (59.3%) as compared to the controls (20.5%, Pc less than 0.005). The relative risk for HLA-B8 was 5.64. No correlation could be demonstrated to specific organ involvement, immunopathological data, age of onset or prognosis.", "contents": "HLA-antigens in systemic lupus erythematosus (SLE). Histocompatibility antigens were determined in 27 patients with SLE and 283 normal blood donors. HLA-B8 was significantly increased in patients with SLE (59.3%) as compared to the controls (20.5%, Pc less than 0.005). The relative risk for HLA-B8 was 5.64. No correlation could be demonstrated to specific organ involvement, immunopathological data, age of onset or prognosis."}
{"id": "PMID:464653", "title": "Lymphocyte response to mitogens in patients with malignant melanoma.", "content": "Lymphocyte blast transformation of 23 melanoma patients was compared to that of 22 healthy persons after stimulation with the mitogens PHA, ConA, and PWM. Transformation rate of lymphocytes in microcultures was measured following 3H-Thymidin uptake in a Liquid-Scintillation-Counter. Calculations were based on analyses of variance. There was no significant difference in blastogenetic response between the 2 groups (patients and controls), but there were differences between the used mitogens.", "contents": "Lymphocyte response to mitogens in patients with malignant melanoma. Lymphocyte blast transformation of 23 melanoma patients was compared to that of 22 healthy persons after stimulation with the mitogens PHA, ConA, and PWM. Transformation rate of lymphocytes in microcultures was measured following 3H-Thymidin uptake in a Liquid-Scintillation-Counter. Calculations were based on analyses of variance. There was no significant difference in blastogenetic response between the 2 groups (patients and controls), but there were differences between the used mitogens."}
{"id": "PMID:464654", "title": "Prealbumin as a hapten carrier protein in certain varieties of dermatitis.", "content": "A significant decrease of serum prealbumin levels and an increased proportion of prealbumin bearing lymphocytes in peripheral blood have been found in patients of hapten-induced diseases. Prealbumin deposition in the vessel walls, and/or prealbumin positive cells (granulocytes, lymphocytes, macrophages) in the dermis have been observed in patients with proved drug allergy as well as allergic contact dermatitis using immunofluorescence technique. The eluates from membrane of lymphocytes of patients with drug allergy contained a mitogenic substance, probably antigen and sometimes prealbumin as well. These results also support the earlier suggested hypothesis that the prealbumin may act as a hapten carrier protein.", "contents": "Prealbumin as a hapten carrier protein in certain varieties of dermatitis. A significant decrease of serum prealbumin levels and an increased proportion of prealbumin bearing lymphocytes in peripheral blood have been found in patients of hapten-induced diseases. Prealbumin deposition in the vessel walls, and/or prealbumin positive cells (granulocytes, lymphocytes, macrophages) in the dermis have been observed in patients with proved drug allergy as well as allergic contact dermatitis using immunofluorescence technique. The eluates from membrane of lymphocytes of patients with drug allergy contained a mitogenic substance, probably antigen and sometimes prealbumin as well. These results also support the earlier suggested hypothesis that the prealbumin may act as a hapten carrier protein."}
{"id": "PMID:464658", "title": "Ichthyosis nigricans: ultrastructural study of the melanin pigmentary disturbances.", "content": "Hyperpigmented skin from a 10-year-old white boy with ichthyosis nigricans has been studied. Histological and ultrastructural studies reveal that the hyperpigmentation is related to both epidermal and dermal hypermelanosis. Melanocytes are hyperactive. The different stages of melanosome synthesis and melanisation appear to be normal. Increased dermal pigmentation probably results from a dischargement of the melanin granules into the dermis secondary to melanocyte alterations. Ichthyosis nigricans is a typical example of melanin pigmentary disturbances of the skin, resulting from disturbances in the normal interactions between melanocytes and keratinocytes.", "contents": "Ichthyosis nigricans: ultrastructural study of the melanin pigmentary disturbances. Hyperpigmented skin from a 10-year-old white boy with ichthyosis nigricans has been studied. Histological and ultrastructural studies reveal that the hyperpigmentation is related to both epidermal and dermal hypermelanosis. Melanocytes are hyperactive. The different stages of melanosome synthesis and melanisation appear to be normal. Increased dermal pigmentation probably results from a dischargement of the melanin granules into the dermis secondary to melanocyte alterations. Ichthyosis nigricans is a typical example of melanin pigmentary disturbances of the skin, resulting from disturbances in the normal interactions between melanocytes and keratinocytes."}
{"id": "PMID:464659", "title": "Urinary polyamine levels in patients with psoriasis.", "content": "Polyamine contents in 24 h urine of 16 psoriatic patients and seven healthy individuals are measured. The average values of putrescine and spermine showed slight increase in the psoriatic group, while those of spermidine were unchanged. When psoriatic patients are divided into three groups according to the extent of their skin lesion, the putrescine level is found to be higher in the group more severely affected.", "contents": "Urinary polyamine levels in patients with psoriasis. Polyamine contents in 24 h urine of 16 psoriatic patients and seven healthy individuals are measured. The average values of putrescine and spermine showed slight increase in the psoriatic group, while those of spermidine were unchanged. When psoriatic patients are divided into three groups according to the extent of their skin lesion, the putrescine level is found to be higher in the group more severely affected."}
{"id": "PMID:464660", "title": "Autoradiographic study on some proliferative properties of human buccal mucosa.", "content": "This study was designed to investigate the proliferative activity of the buccal mucosa in healthy adult persons with regard to individual age. Punch biopsy specimens were taken from the buccal mucosa of 19 individuals (10 females, 9 males) ranging in age from 24 to 80 years, and were then examined autoradiographically, applying nuclear 3H-thymidine labelling in vitro under oxygen pressure of 2.2 atm. The mean total labelling index in the progenitor compartment amounted to 26.0 +/- 2.6% including 6.2 +/- 2.0% labelled basal nuclei and 19.8 +/- 2.6% labelled suprabasal nuclei. Related to 1,000 mu surface length, the mean number of basal cells runs up to 270.7 +/- 38.6 and the total nuclear labelling index to 70.9 +/- 10.4. This high rate of S phase nuclei, exceeding that of the epidermis several times, depends on the ample progenitor compartment as well as on the basal epithelium length which, as compared to the epithelium surface length, was shown to be extended by a mean proportion of 1.75. None of the autoradiographic results obtained in the present study revealed an age dependency of the buccal mucosa proliferative activity. The magnitude of the proliferative pool constitutes the matrix of the high renewal rate of the healthy buccal mucosa and provides a steady state of superficial cell loss and basal cell regeneration, that was proved, at least in the normal mucosa, to continue until senile age.", "contents": "Autoradiographic study on some proliferative properties of human buccal mucosa. This study was designed to investigate the proliferative activity of the buccal mucosa in healthy adult persons with regard to individual age. Punch biopsy specimens were taken from the buccal mucosa of 19 individuals (10 females, 9 males) ranging in age from 24 to 80 years, and were then examined autoradiographically, applying nuclear 3H-thymidine labelling in vitro under oxygen pressure of 2.2 atm. The mean total labelling index in the progenitor compartment amounted to 26.0 +/- 2.6% including 6.2 +/- 2.0% labelled basal nuclei and 19.8 +/- 2.6% labelled suprabasal nuclei. Related to 1,000 mu surface length, the mean number of basal cells runs up to 270.7 +/- 38.6 and the total nuclear labelling index to 70.9 +/- 10.4. This high rate of S phase nuclei, exceeding that of the epidermis several times, depends on the ample progenitor compartment as well as on the basal epithelium length which, as compared to the epithelium surface length, was shown to be extended by a mean proportion of 1.75. None of the autoradiographic results obtained in the present study revealed an age dependency of the buccal mucosa proliferative activity. The magnitude of the proliferative pool constitutes the matrix of the high renewal rate of the healthy buccal mucosa and provides a steady state of superficial cell loss and basal cell regeneration, that was proved, at least in the normal mucosa, to continue until senile age."}
{"id": "PMID:464661", "title": "Microcalorimetric investigations of the metabolism of isolated human epidermis.", "content": "Microcalorimetry was used for the evaluation of metabolic and enzyme activity in human epidermis. The endogenous metabolism with a heat production Q = -2 muW/mg wet weight could be stimulated to Q = -12 muW/mg by adding glucose to the buffer. To test the enzyme activity, hydrogenation of pyruvate to lactate by lactate dehydrogenase (LDH) was chosen as an essential step of anaerobic glycolysis. The LDH activity of epidermis slices amounted to 22 mU/mg epidermis. The microcalorimetric method is discussed as a test procedure for specimens of healthy and diseased skin and for cell cultures.", "contents": "Microcalorimetric investigations of the metabolism of isolated human epidermis. Microcalorimetry was used for the evaluation of metabolic and enzyme activity in human epidermis. The endogenous metabolism with a heat production Q = -2 muW/mg wet weight could be stimulated to Q = -12 muW/mg by adding glucose to the buffer. To test the enzyme activity, hydrogenation of pyruvate to lactate by lactate dehydrogenase (LDH) was chosen as an essential step of anaerobic glycolysis. The LDH activity of epidermis slices amounted to 22 mU/mg epidermis. The microcalorimetric method is discussed as a test procedure for specimens of healthy and diseased skin and for cell cultures."}
{"id": "PMID:464662", "title": "Pure melanocyte cultures: differential serum requirements of guinea pig keratinocytes and melanocytes in primary epidermal cell cultures.", "content": "Contrary to melanocytes guinea pig keratinocytes do not attach and grow in primary epidermal cell cultures if plated in media containing guinea pig serum. This phenomenon is based on the low content or lack of guinea pig serum of a factor(s) promoting keratinocyte attachment. This factor, which is contained in fetal calf serum, binds to the keratinocyte cell surface and can be removed by trypsin. Guinea pig epidermal cell cultures plated in medium containing guinea pig serum therefore lead to pure or almost pure melanocyte cultures.", "contents": "Pure melanocyte cultures: differential serum requirements of guinea pig keratinocytes and melanocytes in primary epidermal cell cultures. Contrary to melanocytes guinea pig keratinocytes do not attach and grow in primary epidermal cell cultures if plated in media containing guinea pig serum. This phenomenon is based on the low content or lack of guinea pig serum of a factor(s) promoting keratinocyte attachment. This factor, which is contained in fetal calf serum, binds to the keratinocyte cell surface and can be removed by trypsin. Guinea pig epidermal cell cultures plated in medium containing guinea pig serum therefore lead to pure or almost pure melanocyte cultures."}
{"id": "PMID:464663", "title": "Lymphocyte proliferation during PUVA therapy.", "content": "The lymphocyte proliferation of 15 psoriatic patients was sutdied after stimulation with tuberculin, trichophytin, varidase, concanavalin A (Con A), and pokeweek mitogen (PMW) during the first and the eighth PUVA treatment, 2 h after oral intake of 8-methoxypsoralen (8-MOP), immediately after UVA-irradiation, and 24 h later. There was a significant decrease of the lymphocyte proliferation, after stimulation with Con A (P less than 0.05), trichophytin (P less than 0.01) and varidase (P less than 0.05), not with PWM and tuberculin, during the first PUVA treatment by 8-MOP alone. This decrease was not significantly changed by the following UVA irradiation and could not be demonstrated after 24 h. No dark effect of 8-MOP on the lymphocyte proliferation could be seen during the eighth treatment. 8-MOP seems to influence lymphocyte proliferation in the dark by interfering with the cell surface, not with the nucleus. The disappearence of this effect during PUVA therapy could be explained by a modification on the surface structure of lymphocytes.", "contents": "Lymphocyte proliferation during PUVA therapy. The lymphocyte proliferation of 15 psoriatic patients was sutdied after stimulation with tuberculin, trichophytin, varidase, concanavalin A (Con A), and pokeweek mitogen (PMW) during the first and the eighth PUVA treatment, 2 h after oral intake of 8-methoxypsoralen (8-MOP), immediately after UVA-irradiation, and 24 h later. There was a significant decrease of the lymphocyte proliferation, after stimulation with Con A (P less than 0.05), trichophytin (P less than 0.01) and varidase (P less than 0.05), not with PWM and tuberculin, during the first PUVA treatment by 8-MOP alone. This decrease was not significantly changed by the following UVA irradiation and could not be demonstrated after 24 h. No dark effect of 8-MOP on the lymphocyte proliferation could be seen during the eighth treatment. 8-MOP seems to influence lymphocyte proliferation in the dark by interfering with the cell surface, not with the nucleus. The disappearence of this effect during PUVA therapy could be explained by a modification on the surface structure of lymphocytes."}
{"id": "PMID:464664", "title": "Effects of glucocorticosteroids on cultured human skin fibroblasts. III. Transient inhibition of cell proliferation in the early growth stages and reduced susceptibility in later growth stages.", "content": "An immediate depression of the rate of cell proliferation occurred upon addition of glucocorticosteroids to cultures of human skin fibroblasts in the early growth stages. A reduced sensitivity or even insensitivity of the fibroblasts to growth inhibition inhibition was found upon the addition of the steroids at later stages of cell growth, when the cell density has increased. The inhibition in the early growth stages is transient and is most pronounced if the cultured medium is not renewed. This transient inhibition is not due to the development of steroid-resistant cell lines, and resembles the effect called tachyphylaxis, as is also observed in vasoconstriction tests.", "contents": "Effects of glucocorticosteroids on cultured human skin fibroblasts. III. Transient inhibition of cell proliferation in the early growth stages and reduced susceptibility in later growth stages. An immediate depression of the rate of cell proliferation occurred upon addition of glucocorticosteroids to cultures of human skin fibroblasts in the early growth stages. A reduced sensitivity or even insensitivity of the fibroblasts to growth inhibition inhibition was found upon the addition of the steroids at later stages of cell growth, when the cell density has increased. The inhibition in the early growth stages is transient and is most pronounced if the cultured medium is not renewed. This transient inhibition is not due to the development of steroid-resistant cell lines, and resembles the effect called tachyphylaxis, as is also observed in vasoconstriction tests."}
{"id": "PMID:464665", "title": "[Blind ureters. Proposed classification and study of the national statistics with the contribution of 2 cases].", "content": "The authors put forward the bases for a classification of the different kinds of B.U., according to the direction which it takes, the situation of the blind end and the accompanying, renoureteral malformations in the system itself. They review the Spanish statistics in which 38 cases of 6 kinds are compiled, included among the 15 in the classification suggested, as well as presenting two of their own. They refer to the possible ischemic cause of this anomaly.", "contents": "[Blind ureters. Proposed classification and study of the national statistics with the contribution of 2 cases]. The authors put forward the bases for a classification of the different kinds of B.U., according to the direction which it takes, the situation of the blind end and the accompanying, renoureteral malformations in the system itself. They review the Spanish statistics in which 38 cases of 6 kinds are compiled, included among the 15 in the classification suggested, as well as presenting two of their own. They refer to the possible ischemic cause of this anomaly."}
{"id": "PMID:464666", "title": "[Massive kidney infarct by occlusion of the main artery].", "content": "Massive kidney infarct, due to total occlusion of the main artery, is not a frequent process in clinical urology. The most frequent causes are endocarditis, arteritis, atheromatosis and traumatisms. The complete blockage of the renal artery means that the tissue irrigated by the same is bloodless and prone to necrosis and it must be taken into account that although the renal parenchyma cannot withstand for more than 1 to 2 hours the lack of a blood supply, the obstructions or ischemias of shorter duration cause tissue disorders of greater or lesser importance, affecting more quickly and more intensely the cells of the tubules, than those of the glomerules and later the connecting tissue. Clinically, kidney infarcts may sometimes go unobserved and on many other occasions their symptoms are by no means typical although the most characteristic feature is a more intense, sharp, acute pain with macroscopic hematuria, proteinuria and cylindruria and, in the radiological exploration, kidney \"silence\" but with the excretory duct intact shown by means of retrograde uretero-pyelography. The kidney angiography will reveal the existence of the arterial obstruction, with the resulting avascular image. Extrapremature surgical treatment would be ideal in the cases of massive infarct but this would also require an extrapremature diagnosis, which would enable the embolectomy (where necessary to be carried out, thereby saving the kidney. However, under normal working conditions, taking into account the period of time which inevitably elapses between the patient feeling pain in the kidney and his reaching the Emergency Department and the necessary examinations being carried out which enable the correct diagnosis to be made, the number of hours which have passed make attempts at conservative surgery completely useless. The authors present the case of a 37-year old patient who, 15 days after presenting a picture of right kidney colic, went to the Emergency Department in our Centre where the doctor on duty merely performed a symptomatic treatment and the patient was not admitted to our Department until several days later. In the different radiourographic examinations carried out, right kidney mutism was observed, as well as the permeability of the excretory duct. The aortography revealed the total occlusion of the right renal artery. As more than 20 days had elapsed since the patient first presented the colic pain and before we examined him, there was no other therapeutic solution but the performing of a nephrectomy. The examination of the organ  removed confirmed the diagnosis but the origin of the arterial obstruction could not be clarified for sure.", "contents": "[Massive kidney infarct by occlusion of the main artery]. Massive kidney infarct, due to total occlusion of the main artery, is not a frequent process in clinical urology. The most frequent causes are endocarditis, arteritis, atheromatosis and traumatisms. The complete blockage of the renal artery means that the tissue irrigated by the same is bloodless and prone to necrosis and it must be taken into account that although the renal parenchyma cannot withstand for more than 1 to 2 hours the lack of a blood supply, the obstructions or ischemias of shorter duration cause tissue disorders of greater or lesser importance, affecting more quickly and more intensely the cells of the tubules, than those of the glomerules and later the connecting tissue. Clinically, kidney infarcts may sometimes go unobserved and on many other occasions their symptoms are by no means typical although the most characteristic feature is a more intense, sharp, acute pain with macroscopic hematuria, proteinuria and cylindruria and, in the radiological exploration, kidney \"silence\" but with the excretory duct intact shown by means of retrograde uretero-pyelography. The kidney angiography will reveal the existence of the arterial obstruction, with the resulting avascular image. Extrapremature surgical treatment would be ideal in the cases of massive infarct but this would also require an extrapremature diagnosis, which would enable the embolectomy (where necessary to be carried out, thereby saving the kidney. However, under normal working conditions, taking into account the period of time which inevitably elapses between the patient feeling pain in the kidney and his reaching the Emergency Department and the necessary examinations being carried out which enable the correct diagnosis to be made, the number of hours which have passed make attempts at conservative surgery completely useless. The authors present the case of a 37-year old patient who, 15 days after presenting a picture of right kidney colic, went to the Emergency Department in our Centre where the doctor on duty merely performed a symptomatic treatment and the patient was not admitted to our Department until several days later. In the different radiourographic examinations carried out, right kidney mutism was observed, as well as the permeability of the excretory duct. The aortography revealed the total occlusion of the right renal artery. As more than 20 days had elapsed since the patient first presented the colic pain and before we examined him, there was no other therapeutic solution but the performing of a nephrectomy. The examination of the organ  removed confirmed the diagnosis but the origin of the arterial obstruction could not be clarified for sure."}
{"id": "PMID:464671", "title": "Preoperative assessment of resectability for carcinoma of the thoracic esophagus. Part I. Esophagogram and azygogram.", "content": "The length and radiologic type of tumor, esophageal axis and azygogram were examined in 208 patients with carcinoma of the thoracic esophagus who underwent esophageal resection from 1965 to 1975. An histologic examination was performed on all resected specimens. These examinations were used as diagnostic aids to determine the resectability of esophageal carcinoma before surgery. Tumor length did not seem an adequate parameter on deciding resectability; nor did the radiologic type of tumor. Examination of the esophageal axis was in determining resectability. The azygogram gave the most accurate information about resectability of these 4 parameters. The probability of a correct diagnosis regarding differentiation of noncurative a3 lesions from other resectable lesions was 85.6%.", "contents": "Preoperative assessment of resectability for carcinoma of the thoracic esophagus. Part I. Esophagogram and azygogram. The length and radiologic type of tumor, esophageal axis and azygogram were examined in 208 patients with carcinoma of the thoracic esophagus who underwent esophageal resection from 1965 to 1975. An histologic examination was performed on all resected specimens. These examinations were used as diagnostic aids to determine the resectability of esophageal carcinoma before surgery. Tumor length did not seem an adequate parameter on deciding resectability; nor did the radiologic type of tumor. Examination of the esophageal axis was in determining resectability. The azygogram gave the most accurate information about resectability of these 4 parameters. The probability of a correct diagnosis regarding differentiation of noncurative a3 lesions from other resectable lesions was 85.6%."}
{"id": "PMID:464667", "title": "[Zinc in prostatic physiopathology. I. Role of zinc in the physiology and biochemistry of the prostatic gland].", "content": "To sum up the role of zinc in prostatic physiology and biochemistry: a) Zinc is found in normal prostate tissue in a greater concentration than in any other organ in the economy, ranging between 0.70 and 1 mgm/gm. of dry tissue. b) The concentration of prostate liquid and semen is greater than that in any other organic liquid. c) It appears that the prostate gland is a zinc storing organ (or deposit). d) The zinc concentration in prostatic tissue is directly proportional to the amount of alveolar tissue existing in the gland. e) It has been shown that the Zn concentration is greater in the nuclear sediment of the different subcellular fractions of the human prostate gland. f) The prostate gland seems to supply zinc to the human sperm and plays an important role in the metabolic activity of the spermatozoid. g) There seems to be a clear difference between the distribution and absorption of radioactive zinc in the different lobes of the prostate gland. h) Zinc also plays an important part in the enzymatic activity of the prostate gland and in the polymeric organization of the RNA and DNA and is vital for cell division. i) Prostatic Zn has a clear hormonal dependency.", "contents": "[Zinc in prostatic physiopathology. I. Role of zinc in the physiology and biochemistry of the prostatic gland]. To sum up the role of zinc in prostatic physiology and biochemistry: a) Zinc is found in normal prostate tissue in a greater concentration than in any other organ in the economy, ranging between 0.70 and 1 mgm/gm. of dry tissue. b) The concentration of prostate liquid and semen is greater than that in any other organic liquid. c) It appears that the prostate gland is a zinc storing organ (or deposit). d) The zinc concentration in prostatic tissue is directly proportional to the amount of alveolar tissue existing in the gland. e) It has been shown that the Zn concentration is greater in the nuclear sediment of the different subcellular fractions of the human prostate gland. f) The prostate gland seems to supply zinc to the human sperm and plays an important role in the metabolic activity of the spermatozoid. g) There seems to be a clear difference between the distribution and absorption of radioactive zinc in the different lobes of the prostate gland. h) Zinc also plays an important part in the enzymatic activity of the prostate gland and in the polymeric organization of the RNA and DNA and is vital for cell division. i) Prostatic Zn has a clear hormonal dependency."}
{"id": "PMID:464668", "title": "[Air cystometry].", "content": "A better study and more correct diagnosis of neurogenous bladder is to be achieved with cystomanometric studies. The authors analyse the use of air as a medium, explain the method and stress the simplicity, quickness and absence of complications with air. Finally, they mention the recent use of carbon dioxide, particularly in urethral profilometries.", "contents": "[Air cystometry]. A better study and more correct diagnosis of neurogenous bladder is to be achieved with cystomanometric studies. The authors analyse the use of air as a medium, explain the method and stress the simplicity, quickness and absence of complications with air. Finally, they mention the recent use of carbon dioxide, particularly in urethral profilometries."}
{"id": "PMID:464672", "title": "The effects of hypothermia on myocardial oxygen consumption and transmural coronary blood flow in the potassium-arrested heart.", "content": "Hypothermia remains the primary adjunct employed to lower cellular metabolism during various cardiac procedures. In these experiments, left ventricular myocardial oxygen consumption (MVO2) and transmural blood flow (TBF) were measured during cardiopulmonary bypass with the range of temperatures used clinically. Determinations were made in empty beating normothermic hearts and after potassium cardioplegia at 37, 32, 28, 22, 18, and 15 degrees (K+ = 15--37 meq/L: Hct 25 volumes %). Oxygen content of the total coronary sinus collection was compared with a large volume arterial sample using a Lex-O2-Con-TL analyzer (vs Van Slyke, R = 0.98). Transmural blood flow was measured at each temperature using microspheres (8 microns), and perfusion was maintained at 80 mmHg. Asystole (37 degrees) alone decreased MVO2 from 5.18 +/- 0.55 to 1.85 +/- 0.20 ml O2/min/100 g of left ventricle or approximately 65% (p less than 0.001). With progressive cooling to 15 degrees an additional 82% decrement in oxygen uptake occurred during asystole (p less than 0.001). During asystole at 37 degrees the decrease in MVO2 was reflected mainly by a large decrement (p less than 0.01) in TBF (1.27 +/- 0.19 to 0.74 +/- 0.17 ml/min/g of mean left ventricular flow). However, with cooling below 32 degrees, the arteriovenous oxygen difference narrowed progressively (p less than 0.001) while TBF paradoxically returned to control levels. Endocardial/epicardial flow ratios were not altered by cooling. These data not only confirm earlier reports describing a sequential drop in MVO2 with incremental myocardial cooling, but also establish MVO2 levels for perfused hearts arrested by potassium at lower temperatures (18--15 degrees). Moreover, as transmural blood flow becomes independent of metabolic necessity during hypothermia, coronary autoregulation appears to be impaired, possibly affecting detrimental tissue over perfusion.", "contents": "The effects of hypothermia on myocardial oxygen consumption and transmural coronary blood flow in the potassium-arrested heart. Hypothermia remains the primary adjunct employed to lower cellular metabolism during various cardiac procedures. In these experiments, left ventricular myocardial oxygen consumption (MVO2) and transmural blood flow (TBF) were measured during cardiopulmonary bypass with the range of temperatures used clinically. Determinations were made in empty beating normothermic hearts and after potassium cardioplegia at 37, 32, 28, 22, 18, and 15 degrees (K+ = 15--37 meq/L: Hct 25 volumes %). Oxygen content of the total coronary sinus collection was compared with a large volume arterial sample using a Lex-O2-Con-TL analyzer (vs Van Slyke, R = 0.98). Transmural blood flow was measured at each temperature using microspheres (8 microns), and perfusion was maintained at 80 mmHg. Asystole (37 degrees) alone decreased MVO2 from 5.18 +/- 0.55 to 1.85 +/- 0.20 ml O2/min/100 g of left ventricle or approximately 65% (p less than 0.001). With progressive cooling to 15 degrees an additional 82% decrement in oxygen uptake occurred during asystole (p less than 0.001). During asystole at 37 degrees the decrease in MVO2 was reflected mainly by a large decrement (p less than 0.01) in TBF (1.27 +/- 0.19 to 0.74 +/- 0.17 ml/min/g of mean left ventricular flow). However, with cooling below 32 degrees, the arteriovenous oxygen difference narrowed progressively (p less than 0.001) while TBF paradoxically returned to control levels. Endocardial/epicardial flow ratios were not altered by cooling. These data not only confirm earlier reports describing a sequential drop in MVO2 with incremental myocardial cooling, but also establish MVO2 levels for perfused hearts arrested by potassium at lower temperatures (18--15 degrees). Moreover, as transmural blood flow becomes independent of metabolic necessity during hypothermia, coronary autoregulation appears to be impaired, possibly affecting detrimental tissue over perfusion."}
{"id": "PMID:464673", "title": "Infusion of the branched chain amino acids in postoperative patients. Anticatabolic properties.", "content": "Postinjury metabolism is characterized by breakdown of muscle protein as substrate for energy production and gluconeogenesis and by the resultant loss of lean body mass and weight loss. The branched chain amino acids (BCAAs) which are principally oxidized by the skeletal muscle have been implicated in recent in vitro and in vivo studies as having special anticatabolic and regulating effects in skeletal muscle. We studied the anticatabolic effects of the BCAAs in 35 patients undergoing operative injury of moderate severity. In a prospective randomized and blinded manner patients were infused for five days starting immediately after surgery with either 5% dextrose or 5% dextrose with an amino acid solution containing 22, 35 or 100% BCAAs. All patients survived and there were no major postoperative complications. Mean hospital stay was 17 days for patients receiving amino acids and 19 days for patients receiving 5% dextrose only (p = ns). All three groups receiving amino acid solutions were in nitrogen equilibrium or in a slight positive nitrogen balance, while the group receiving 5% dextrose only was in a mean negative nitrogen balance of 6.6 +/- 0.6 gN/day. The differences between the three groups receiving amino acids were slight and not significant. Weight loss was 2 +/- 0.7 kg in the 5% dextrose group, 1 +/- 0.7 kg in the 22% BCAAs group, 0.5 +/- 0.5 kg in the 35% BCAAs group and the 100% BCAAs group gained 0.4 +/- 1.8 kg. Blood chemistries in the different groups and during the study period remained within normal limits except for ammonia levels rising significantly in the 5% dextrose group and SGOT levels rising in the 22% and 35% BCAA groups. With mild variations the plasma amino acid patterns in all groups were similar to the normal pattern, even in the 100% BCAAs group receiving an unbalanced amino acid solution, suggesting the complete cessation of amino acid efflux from muscle, the muscle depending solely on the exogenous supply of BCAAs to satisfy its metabolic requirements. The results suggest that early nutritional suppport in the postoperative period will result in nitrogen equilibrium and that the infusion of the three BCAAs only in the postoperative state is as effective in preventing muscle catabolism as other more balanced amino acid solutions. In the postinjury state balanced amino acid solutions rich in BCAA may prove beneficial.", "contents": "Infusion of the branched chain amino acids in postoperative patients. Anticatabolic properties. Postinjury metabolism is characterized by breakdown of muscle protein as substrate for energy production and gluconeogenesis and by the resultant loss of lean body mass and weight loss. The branched chain amino acids (BCAAs) which are principally oxidized by the skeletal muscle have been implicated in recent in vitro and in vivo studies as having special anticatabolic and regulating effects in skeletal muscle. We studied the anticatabolic effects of the BCAAs in 35 patients undergoing operative injury of moderate severity. In a prospective randomized and blinded manner patients were infused for five days starting immediately after surgery with either 5% dextrose or 5% dextrose with an amino acid solution containing 22, 35 or 100% BCAAs. All patients survived and there were no major postoperative complications. Mean hospital stay was 17 days for patients receiving amino acids and 19 days for patients receiving 5% dextrose only (p = ns). All three groups receiving amino acid solutions were in nitrogen equilibrium or in a slight positive nitrogen balance, while the group receiving 5% dextrose only was in a mean negative nitrogen balance of 6.6 +/- 0.6 gN/day. The differences between the three groups receiving amino acids were slight and not significant. Weight loss was 2 +/- 0.7 kg in the 5% dextrose group, 1 +/- 0.7 kg in the 22% BCAAs group, 0.5 +/- 0.5 kg in the 35% BCAAs group and the 100% BCAAs group gained 0.4 +/- 1.8 kg. Blood chemistries in the different groups and during the study period remained within normal limits except for ammonia levels rising significantly in the 5% dextrose group and SGOT levels rising in the 22% and 35% BCAA groups. With mild variations the plasma amino acid patterns in all groups were similar to the normal pattern, even in the 100% BCAAs group receiving an unbalanced amino acid solution, suggesting the complete cessation of amino acid efflux from muscle, the muscle depending solely on the exogenous supply of BCAAs to satisfy its metabolic requirements. The results suggest that early nutritional suppport in the postoperative period will result in nitrogen equilibrium and that the infusion of the three BCAAs only in the postoperative state is as effective in preventing muscle catabolism as other more balanced amino acid solutions. In the postinjury state balanced amino acid solutions rich in BCAA may prove beneficial."}
{"id": "PMID:464669", "title": "[Total ureteral rupture caused by closed abdominal injury in polytraumatized patients].", "content": "The authors present a case of complete ureteral rupture, after closed, abdominal traumatism in a patient suffering from multiple traumatisms. They point out the slight number of cases published in the literature as well as the frequency of its delayed diagnosis due to few or no symptoms, particularly when associated with visceral ruptures or bone fractures which may conceal the picture. They recommend the I.V.U. for all patients with multiple traumatisms once they have recovered from the state of shock, as a simple exploration but which provides a great deal of information. They also advise conservative treatment even in cases with a delayed diagnosis.", "contents": "[Total ureteral rupture caused by closed abdominal injury in polytraumatized patients]. The authors present a case of complete ureteral rupture, after closed, abdominal traumatism in a patient suffering from multiple traumatisms. They point out the slight number of cases published in the literature as well as the frequency of its delayed diagnosis due to few or no symptoms, particularly when associated with visceral ruptures or bone fractures which may conceal the picture. They recommend the I.V.U. for all patients with multiple traumatisms once they have recovered from the state of shock, as a simple exploration but which provides a great deal of information. They also advise conservative treatment even in cases with a delayed diagnosis."}
{"id": "PMID:464670", "title": "[Conservative therapy of urolithiasis].", "content": "Some common conservative treating methods of urolithiasis are critically discussed. Based on physico-chemical relationships of solubility and cristallisation of stone-forming compounds our own methods of conservative treatment of urolithiasis is presented.", "contents": "[Conservative therapy of urolithiasis]. Some common conservative treating methods of urolithiasis are critically discussed. Based on physico-chemical relationships of solubility and cristallisation of stone-forming compounds our own methods of conservative treatment of urolithiasis is presented."}
{"id": "PMID:464674", "title": "Typhoid perforation of the bowel. Experiences in 78 cases.", "content": "Seventy-eight patients ranging from four to 65 years of age were treated for typhoid perforation of the bowel. Sixty-one patients (78%) were males. The average time from perforation to admission was 56 hours. The mortality rate was 32% and was adversely influenced by the duration of illness, duration of perforation, shock, uremia, encephalopathy and fecal peritonitis. Forty-nine patients were treated by closure of the perforation, resection or miscellaneous procedures; the other 29 by closure of the perforation combined with an end-to-end ileotransverse colostomy. Although mortality was the same in both groups, those undergoing bypass had a significantly smoother postoperative course.", "contents": "Typhoid perforation of the bowel. Experiences in 78 cases. Seventy-eight patients ranging from four to 65 years of age were treated for typhoid perforation of the bowel. Sixty-one patients (78%) were males. The average time from perforation to admission was 56 hours. The mortality rate was 32% and was adversely influenced by the duration of illness, duration of perforation, shock, uremia, encephalopathy and fecal peritonitis. Forty-nine patients were treated by closure of the perforation, resection or miscellaneous procedures; the other 29 by closure of the perforation combined with an end-to-end ileotransverse colostomy. Although mortality was the same in both groups, those undergoing bypass had a significantly smoother postoperative course."}
{"id": "PMID:464675", "title": "Initial treatment of sigmoid volvulous by colonoscopy.", "content": "The initial management of acute, nonstrangulated sigmoid volvulous is to attempt proctosigmoidoscopic, rectal tube, or barium enema reduction and evacuation. If unsuccessful emergency surgery is necessary. The flexible colonoscope offers an additional therapeutic modality to effectuate preoperative reduction of the twisted sigmoid colon if attempts with conventional methods fail. Three cases of acute sigmoid volvulous are presented which illustrate for the first time successful reduction of acute sigmoid volvulous by colonoscopy after failure of the usual methods of treatment. Instead of emergency surgery all of these patients had elective resection with primary colocolostomy. Patients with acute sigmoid volvulous refractile to reduction by conventional modalities should have an attempt at flexible colonoscopic reduction.", "contents": "Initial treatment of sigmoid volvulous by colonoscopy. The initial management of acute, nonstrangulated sigmoid volvulous is to attempt proctosigmoidoscopic, rectal tube, or barium enema reduction and evacuation. If unsuccessful emergency surgery is necessary. The flexible colonoscope offers an additional therapeutic modality to effectuate preoperative reduction of the twisted sigmoid colon if attempts with conventional methods fail. Three cases of acute sigmoid volvulous are presented which illustrate for the first time successful reduction of acute sigmoid volvulous by colonoscopy after failure of the usual methods of treatment. Instead of emergency surgery all of these patients had elective resection with primary colocolostomy. Patients with acute sigmoid volvulous refractile to reduction by conventional modalities should have an attempt at flexible colonoscopic reduction."}
{"id": "PMID:464676", "title": "Megacolon in the elderly. Ischemic or inflammatory?", "content": "Ischemic colitis has been previously described in three forms: transient, strictured, and gangrenous. A fourth form of presentation in the elderly is characterized by signs of an acute abdomen, massive colonic dilatation, and systemic toxicity. Bloody diarrhea may be seen prior to the onset of dilatation. Ischemia should be considered as an etiologic factor in \"colitis\" in the elderly patient with segmental dilatation particularly if it follows a \"low flow state.\" The rectum is usually uninvolved. Barium enema may confirm segmental involvement and later demonstrate stricture. Three patients with ischemic megacolon are presented. The diagnosis was suspected preoperatively in only one. In contrast to ulcerative colitis, these patients show a more abrupt onset and run a fulminant course. In patients who recover, there is lower relapse rate than young patients with ulcerative colitis. When resection is indicated, all attempts should be made to spare the rectum. Loop ileostomy and decompressive colostomy offer an excellent temporizing measure to assist the patient through the acute phase of the illness.", "contents": "Megacolon in the elderly. Ischemic or inflammatory? Ischemic colitis has been previously described in three forms: transient, strictured, and gangrenous. A fourth form of presentation in the elderly is characterized by signs of an acute abdomen, massive colonic dilatation, and systemic toxicity. Bloody diarrhea may be seen prior to the onset of dilatation. Ischemia should be considered as an etiologic factor in \"colitis\" in the elderly patient with segmental dilatation particularly if it follows a \"low flow state.\" The rectum is usually uninvolved. Barium enema may confirm segmental involvement and later demonstrate stricture. Three patients with ischemic megacolon are presented. The diagnosis was suspected preoperatively in only one. In contrast to ulcerative colitis, these patients show a more abrupt onset and run a fulminant course. In patients who recover, there is lower relapse rate than young patients with ulcerative colitis. When resection is indicated, all attempts should be made to spare the rectum. Loop ileostomy and decompressive colostomy offer an excellent temporizing measure to assist the patient through the acute phase of the illness."}
{"id": "PMID:464677", "title": "Evaluation and complications of 107 staging laparotomies for Hodgkin's disease.", "content": "From 1971--1975, 107 staging laparotomies for Hodgkin's disease were performed at the University of Wisconsin Hospitals. Forty-one per cent of patients with abnormal abdominal lymphangiograms preoperatively had abdominal nodes which were negative for Hodgkin's. Thirteen per cent of patients with negative preoperative lymphangiograms had positive nodes at staging laparotomy. Twenty-nine per cent were upstaged by laparotomy, i.e. assigned to a less favorable stage (II A to III A), and 11.2% were downstaged. There were no surgical mortalities. Minor surgical complications occurred in 14.9%, and major ones in 3.7%. We conclude that surgical staging for Hodgkin's disease is valuable in making an accurate diagnosis and, hence, in determining the most effective treatment.", "contents": "Evaluation and complications of 107 staging laparotomies for Hodgkin's disease. From 1971--1975, 107 staging laparotomies for Hodgkin's disease were performed at the University of Wisconsin Hospitals. Forty-one per cent of patients with abnormal abdominal lymphangiograms preoperatively had abdominal nodes which were negative for Hodgkin's. Thirteen per cent of patients with negative preoperative lymphangiograms had positive nodes at staging laparotomy. Twenty-nine per cent were upstaged by laparotomy, i.e. assigned to a less favorable stage (II A to III A), and 11.2% were downstaged. There were no surgical mortalities. Minor surgical complications occurred in 14.9%, and major ones in 3.7%. We conclude that surgical staging for Hodgkin's disease is valuable in making an accurate diagnosis and, hence, in determining the most effective treatment."}
{"id": "PMID:464678", "title": "Regeneration of human liver after hepatic lobectomy studied by repeated liver scanning and repeated needle biopsy.", "content": "Regeneration of the residual lobe of the liver after hepatic lobectomy in humans was studied by repeated liver scanning in seven noncirrhotic and three cirrhotic patients. Each patient was studied for several months during the study which lasted from 1-12 years. Regeneration was apparent in noncirrhotic liver remnants following hepatic lobectomy. In the case of a long standing, space occupying lesions such as benign giant cysts, the liver remnant would complete its regeneration process rather early, usually within a few months of hepatic lobectomy. In hepatoma cases, however, regeneration of the residual lobe after hepatic resection usually took five or six months for completion. On the contrary, no definite increase in the size of the liver remnant was seen on repeated liver scanning in cirrhotic patients. Histologic study of the residual lobe was repeated on needle biopsy specimens in two noncirrhotic and four cirrhotic patients. Regenerative hyperplasia of liver cells with large hyperchromatic, or double nuclei never seen in the preresection liver appeared in the liver remnant five, 11, and 27 days after hepatic lobectomy in noncirrhotic patients. In cirrhotics, however, there were no histologic changes between the preresection liver and the postresection remnant studied three, five, 15, 40 days or even two years and 8 months after hepatic lobectomy.", "contents": "Regeneration of human liver after hepatic lobectomy studied by repeated liver scanning and repeated needle biopsy. Regeneration of the residual lobe of the liver after hepatic lobectomy in humans was studied by repeated liver scanning in seven noncirrhotic and three cirrhotic patients. Each patient was studied for several months during the study which lasted from 1-12 years. Regeneration was apparent in noncirrhotic liver remnants following hepatic lobectomy. In the case of a long standing, space occupying lesions such as benign giant cysts, the liver remnant would complete its regeneration process rather early, usually within a few months of hepatic lobectomy. In hepatoma cases, however, regeneration of the residual lobe after hepatic resection usually took five or six months for completion. On the contrary, no definite increase in the size of the liver remnant was seen on repeated liver scanning in cirrhotic patients. Histologic study of the residual lobe was repeated on needle biopsy specimens in two noncirrhotic and four cirrhotic patients. Regenerative hyperplasia of liver cells with large hyperchromatic, or double nuclei never seen in the preresection liver appeared in the liver remnant five, 11, and 27 days after hepatic lobectomy in noncirrhotic patients. In cirrhotics, however, there were no histologic changes between the preresection liver and the postresection remnant studied three, five, 15, 40 days or even two years and 8 months after hepatic lobectomy."}
{"id": "PMID:464679", "title": "Carcinoid tumors of the appendix. Mesoappendiceal extension and nodal metastases.", "content": "Carcinoid of the appendix occurred in 92 of 400,000 surgical pathology specimens covering 70 years, and followed previously reported incidences by age, sex, symptoms, size, localization and histology. Invasion of the mesoappendix occurred in 13 cases, and in two of these, a reoperative ileocolectomy demonstrated regional node metastases. No distant metastases or fatalities appeared. Tumor size correlated with mesoappendiceal invasion. Carcinoid invasion of the mesoappendix may provide a valuable marker for more distant tumor spread, and justify an aggressive surgical approach in selected patients.", "contents": "Carcinoid tumors of the appendix. Mesoappendiceal extension and nodal metastases. Carcinoid of the appendix occurred in 92 of 400,000 surgical pathology specimens covering 70 years, and followed previously reported incidences by age, sex, symptoms, size, localization and histology. Invasion of the mesoappendix occurred in 13 cases, and in two of these, a reoperative ileocolectomy demonstrated regional node metastases. No distant metastases or fatalities appeared. Tumor size correlated with mesoappendiceal invasion. Carcinoid invasion of the mesoappendix may provide a valuable marker for more distant tumor spread, and justify an aggressive surgical approach in selected patients."}
{"id": "PMID:464680", "title": "Sensitivity of the canine pancreatic intraductal pressure to subclinical reduction in cholinesterase acitivity.", "content": "As a continuation of work from this laboratory on anticholinesterase induced pancreatitis, a study of the relationship between reduced serum cholinesterase activity and changes in pancreatic intraductal pressure was undertaken. Pharmakokinetic studies in three dogs revealed rapid reduction in serum cholinesterase activity following an IV bolus dose of the cholinesterase inhibitor 0,0-diethyl-0-(2-isopropyl-6-methyl-4-pyrimidinyl)phosphorothioate. Following each dose of cholinesterase inhibitor, stable levels of cholinesterase inhibition were reached in 30 minutes. In four dogs the pancreatic duct was perfused from the tail of the ventral pancreas and intraductal pressures measured. A total of 25 mg/kg of the cholinesterase inhibitor was given in 5 mg/kg doses 30 minutes apart, and serum cholinesterase measured 30 minutes after each dose. Mean pressures were established over a 15 minute interval. Linear regression analysis of 23 data points revealed a significant (p < 0.001) cumulative dose-related increase in pancreatic intraductal pressure [Pressure (cm saline) = 14.2 + 1.03 x Cumulative Dose (mg/kg)] and significant (p< 0.001) negative correlation between serum cholinesterase activity and intraductal pressure [Pressure (cm saline) = 48.0 - 0.057 x Esterase Activity (mU/ml)]. These data suggest that, in dogs, reduced cholinesterase activity is directly related to increased pancreatic intraductal pressure, and it may be a factor in the pathogenesis of pancreatitis.", "contents": "Sensitivity of the canine pancreatic intraductal pressure to subclinical reduction in cholinesterase acitivity. As a continuation of work from this laboratory on anticholinesterase induced pancreatitis, a study of the relationship between reduced serum cholinesterase activity and changes in pancreatic intraductal pressure was undertaken. Pharmakokinetic studies in three dogs revealed rapid reduction in serum cholinesterase activity following an IV bolus dose of the cholinesterase inhibitor 0,0-diethyl-0-(2-isopropyl-6-methyl-4-pyrimidinyl)phosphorothioate. Following each dose of cholinesterase inhibitor, stable levels of cholinesterase inhibition were reached in 30 minutes. In four dogs the pancreatic duct was perfused from the tail of the ventral pancreas and intraductal pressures measured. A total of 25 mg/kg of the cholinesterase inhibitor was given in 5 mg/kg doses 30 minutes apart, and serum cholinesterase measured 30 minutes after each dose. Mean pressures were established over a 15 minute interval. Linear regression analysis of 23 data points revealed a significant (p < 0.001) cumulative dose-related increase in pancreatic intraductal pressure [Pressure (cm saline) = 14.2 + 1.03 x Cumulative Dose (mg/kg)] and significant (p< 0.001) negative correlation between serum cholinesterase activity and intraductal pressure [Pressure (cm saline) = 48.0 - 0.057 x Esterase Activity (mU/ml)]. These data suggest that, in dogs, reduced cholinesterase activity is directly related to increased pancreatic intraductal pressure, and it may be a factor in the pathogenesis of pancreatitis."}
{"id": "PMID:464681", "title": "The undescended \"parathymus\". An occasional cause of failed neck exploration for hyperparathyroidism.", "content": "Arrested descent of the inferior parathyroid gland (\"parathymus\") during embryologic development may leave the gland stranded high in the neck. Adenomas of such undescended glands are a rare cause of primary hyperparathyroidism, but they may not be uncommon among patients who have already had a failed cervical exploration. Unless the surgeon is aware of this entity, he will probably overlook it. If one is to find these tumors, it is necessary to extend the dissection upward above the superior pole of the thyroid gland, sometimes as far as the angle of the jaw. Adenomas of undescended \"parathymus\" glands were encountered in seven patients during a recent 20 month (May 1975 through December 1976) surgical experience encompassing 414 primary operations and 27 reoperations for hyperparathyroidism. These tumors were found at initial exploration in one patient and at reoperation in six patients. The individual case histories are presented to illustrate the difficulties posed by this anatomic variant.", "contents": "The undescended \"parathymus\". An occasional cause of failed neck exploration for hyperparathyroidism. Arrested descent of the inferior parathyroid gland (\"parathymus\") during embryologic development may leave the gland stranded high in the neck. Adenomas of such undescended glands are a rare cause of primary hyperparathyroidism, but they may not be uncommon among patients who have already had a failed cervical exploration. Unless the surgeon is aware of this entity, he will probably overlook it. If one is to find these tumors, it is necessary to extend the dissection upward above the superior pole of the thyroid gland, sometimes as far as the angle of the jaw. Adenomas of undescended \"parathymus\" glands were encountered in seven patients during a recent 20 month (May 1975 through December 1976) surgical experience encompassing 414 primary operations and 27 reoperations for hyperparathyroidism. These tumors were found at initial exploration in one patient and at reoperation in six patients. The individual case histories are presented to illustrate the difficulties posed by this anatomic variant."}
{"id": "PMID:464682", "title": "Comparison of estrogen receptor levels in primary and regional metastatic carcinoma of the breast.", "content": "The estrogen receptor (ER) level in carcinoma of the breast is a useful predictor of response to hormonal therapy. Metastatic disease may not have the same level of ER as the primary. In a series of 37 patients who had simultaneous ER determination in both primary neoplasm and regional nodal metastasis there was 81% agreement. The true ER character of the tumor would have been missed in seven patients if only the primary tumor had been sampled. The possible reasons for this discrepancy are discussed. We feel that the nodal metastatic deposit may reflect the true nature of the ER status because it represents a purer concentration of tumor cells as well as representing the aggressive element of the tumor.", "contents": "Comparison of estrogen receptor levels in primary and regional metastatic carcinoma of the breast. The estrogen receptor (ER) level in carcinoma of the breast is a useful predictor of response to hormonal therapy. Metastatic disease may not have the same level of ER as the primary. In a series of 37 patients who had simultaneous ER determination in both primary neoplasm and regional nodal metastasis there was 81% agreement. The true ER character of the tumor would have been missed in seven patients if only the primary tumor had been sampled. The possible reasons for this discrepancy are discussed. We feel that the nodal metastatic deposit may reflect the true nature of the ER status because it represents a purer concentration of tumor cells as well as representing the aggressive element of the tumor."}
{"id": "PMID:464683", "title": "Anoxic hypothermic cardioplegia compared to intermittent anoxic fibrillatory cardiac arrest. Clinical and metabolic experience with 1080 patients.", "content": "Appropriately applied, hypothermic cardioplegia allows an excellent surgical setting that can significantly reduce the myocardial ischemic injury resulting from anoxia. One thousand eighty adult and pediatric patients underwent a variety of corrective cardial surgical procedures utilizing cold potassium cardioplegic solution injected into the coronary arteries via the aortic root. Myocardial septal temperature was maintained at 18--20 degrees during arrested time. This group of patients was compared to a group of 220 patients that underwent intermittent normothermic ischemic arrest to perform cardiac surgical procedures. Significant reduction in morbidity, mortality, perioperative myocardial infarction was noted in favor of the cardioplegic group. Metabolic coronary sinus blood analysis in the group undergoing surgery with cardioplegia revealed favorable changes in myocardial lactate and oxygen extraction.", "contents": "Anoxic hypothermic cardioplegia compared to intermittent anoxic fibrillatory cardiac arrest. Clinical and metabolic experience with 1080 patients. Appropriately applied, hypothermic cardioplegia allows an excellent surgical setting that can significantly reduce the myocardial ischemic injury resulting from anoxia. One thousand eighty adult and pediatric patients underwent a variety of corrective cardial surgical procedures utilizing cold potassium cardioplegic solution injected into the coronary arteries via the aortic root. Myocardial septal temperature was maintained at 18--20 degrees during arrested time. This group of patients was compared to a group of 220 patients that underwent intermittent normothermic ischemic arrest to perform cardiac surgical procedures. Significant reduction in morbidity, mortality, perioperative myocardial infarction was noted in favor of the cardioplegic group. Metabolic coronary sinus blood analysis in the group undergoing surgery with cardioplegia revealed favorable changes in myocardial lactate and oxygen extraction."}
{"id": "PMID:464684", "title": "Seeding arterial prostheses with vascular endothelium. The nature of the lining.", "content": "Arterial prostheses seeded with autogenous vascular endothelium demonstrate a well-organized, cellular, inner lining. To determine the nature of the lining cells, six animals underwent replacement of the infrarenal aorta with Dacron prostheses. During the preparation of three such grafts, endothelium was scraped from the saphenous vein with a steel wool pledget, suspended in chilled Sack's solution, and mixed with blood used to preclot the graft. This suspension was omitted from the three control grafts. After six weeks, the grafts were removed, rinsed and examined. Fluorescent Factor VIII related antigen (F VIII-RA) strongly stained the lining cells. Silver nitrate Ha\u00fctchen and electron microscopy preparations revealed a lining pattern characteristic of vascular endothelium. Endothelial cell-specific Weibel-Palade bodies were identified in the lining cell cytoplasm. Masson's trichrome staining revealed a relatively collagen-poor connective tissue within the seeded fabric. Transmission electron microscopy disclosed vascular smooth muscle cells between the seeded graft fabric and the lining cells. Vasa vasorum, arising from the outer capsule, penetrated the fabric to supply the inner capsules of the seeded grafts. It is concluded that the cells lining seeded canine arterial prostheses are true vascular endothelium supported by vascular smooth muscle cells, that the lining contains minimal connective tissue, and that vasa vasorum develop. Unseeded control grafts lacked these features.", "contents": "Seeding arterial prostheses with vascular endothelium. The nature of the lining. Arterial prostheses seeded with autogenous vascular endothelium demonstrate a well-organized, cellular, inner lining. To determine the nature of the lining cells, six animals underwent replacement of the infrarenal aorta with Dacron prostheses. During the preparation of three such grafts, endothelium was scraped from the saphenous vein with a steel wool pledget, suspended in chilled Sack's solution, and mixed with blood used to preclot the graft. This suspension was omitted from the three control grafts. After six weeks, the grafts were removed, rinsed and examined. Fluorescent Factor VIII related antigen (F VIII-RA) strongly stained the lining cells. Silver nitrate Ha\u00fctchen and electron microscopy preparations revealed a lining pattern characteristic of vascular endothelium. Endothelial cell-specific Weibel-Palade bodies were identified in the lining cell cytoplasm. Masson's trichrome staining revealed a relatively collagen-poor connective tissue within the seeded fabric. Transmission electron microscopy disclosed vascular smooth muscle cells between the seeded graft fabric and the lining cells. Vasa vasorum, arising from the outer capsule, penetrated the fabric to supply the inner capsules of the seeded grafts. It is concluded that the cells lining seeded canine arterial prostheses are true vascular endothelium supported by vascular smooth muscle cells, that the lining contains minimal connective tissue, and that vasa vasorum develop. Unseeded control grafts lacked these features."}
{"id": "PMID:464685", "title": "Hemostasis in massively transfused trauma patients.", "content": "Twenty-seven patients requiring massive transfusions were studied prospectively to determine whether administration of stored, modified whole blood induced a primary disorder of hemostasis evidenced by generalized microvascular oozing. Platelet counts fell in proportion to the number of units of blood transfused. In contrast, the levels of factors V and VIII correlated poorly with the units of blood transfused, 85% of the total variation in the levels being due to influences other than transfused blood. Levels of all other clotting factors were unrelated to the number of units of blood given. Eight patients developed abnormal bleeding. The cause appeared to be dilutional thrombocytopenia in five patients, and DIC in three. In six of the eight, bleeding was controlled with platelet concentrates alone. Two patients were given cryoprecipitate also. The most useful laboratory test for predicting abnormal bleeding was the platelet count. Fibrinogen levels should be followed as an aid in the diagnosis of DIC. The BT, PT, and PTT were not helpful in assessing the cause of bleeding, unless they were greater than 1.5 times the control value. We recommend that any patient receiving massive transfusions who develops diffuse microvascular bleeding be given platelet concentrates. Platelet counts as high as 100,000 may be required to control bleeding from surgical wounds. It is not necessary to supplement transfusions of stored, modified whole blood with fresh blood or fresh frozen plasma.", "contents": "Hemostasis in massively transfused trauma patients. Twenty-seven patients requiring massive transfusions were studied prospectively to determine whether administration of stored, modified whole blood induced a primary disorder of hemostasis evidenced by generalized microvascular oozing. Platelet counts fell in proportion to the number of units of blood transfused. In contrast, the levels of factors V and VIII correlated poorly with the units of blood transfused, 85% of the total variation in the levels being due to influences other than transfused blood. Levels of all other clotting factors were unrelated to the number of units of blood given. Eight patients developed abnormal bleeding. The cause appeared to be dilutional thrombocytopenia in five patients, and DIC in three. In six of the eight, bleeding was controlled with platelet concentrates alone. Two patients were given cryoprecipitate also. The most useful laboratory test for predicting abnormal bleeding was the platelet count. Fibrinogen levels should be followed as an aid in the diagnosis of DIC. The BT, PT, and PTT were not helpful in assessing the cause of bleeding, unless they were greater than 1.5 times the control value. We recommend that any patient receiving massive transfusions who develops diffuse microvascular bleeding be given platelet concentrates. Platelet counts as high as 100,000 may be required to control bleeding from surgical wounds. It is not necessary to supplement transfusions of stored, modified whole blood with fresh blood or fresh frozen plasma."}
{"id": "PMID:464686", "title": "Pathologic identification of poor prognosis stage I (T1N0M0) cancer of the breast.", "content": "Twenty to 40% of Stage I(T1N0M0) cancers of the breast recur in ten years. This is an attempt to identify those patients in whom the disease is likely to recur. On the basis of a study of the histologic changes in the tumor and treatment failures poor prognosis was associated with several histological characteristics: poor cytologic differentiation; lymphatic permeation; blood vessel invasion and invasion of the tumor into the surrounding soft tissue. This classification was then applied to 363 cancers of the breast seen over a five year period and followed three to eight years. There were 203 Stage I (T1N0M0) tumors in the group. Ninety-four of the 203 Stage I tumors had one to four of the above histologic characteristics; 109 had none. Among the 109 patients characterized as good risks there were two treatment failures (2%). In the group of 94 with any high risk histologic features there were 47 treatment failures (50%) which were statistically significant (p = 0.001). The histologic changes had a cumulative effect on the degree of malignancy of the tumor. Pathologic changes in the tumor identified those patients whose Stage I (T1N0M0) tumors were likely to recur.", "contents": "Pathologic identification of poor prognosis stage I (T1N0M0) cancer of the breast. Twenty to 40% of Stage I(T1N0M0) cancers of the breast recur in ten years. This is an attempt to identify those patients in whom the disease is likely to recur. On the basis of a study of the histologic changes in the tumor and treatment failures poor prognosis was associated with several histological characteristics: poor cytologic differentiation; lymphatic permeation; blood vessel invasion and invasion of the tumor into the surrounding soft tissue. This classification was then applied to 363 cancers of the breast seen over a five year period and followed three to eight years. There were 203 Stage I (T1N0M0) tumors in the group. Ninety-four of the 203 Stage I tumors had one to four of the above histologic characteristics; 109 had none. Among the 109 patients characterized as good risks there were two treatment failures (2%). In the group of 94 with any high risk histologic features there were 47 treatment failures (50%) which were statistically significant (p = 0.001). The histologic changes had a cumulative effect on the degree of malignancy of the tumor. Pathologic changes in the tumor identified those patients whose Stage I (T1N0M0) tumors were likely to recur."}
{"id": "PMID:464687", "title": "Risk reduction in gastric operations for obesity.", "content": "Although nearly devoid of late complications, gastric operations for obesity have resulted in 4.7% early postoperative perforations. For patients over 39 years of age who perforated, the first 11 patients died and the last 9 survived. Perforations are equally common in upper stomach, anastomosis, and lower stomach. They have become more frequent with the 50 ml upper stomach volume and 12 mm stoma that are required to assure optimum weight control. Perforation is as common with gastroplasty as with gastric bypass. If it occurs, it is normally within the first ten postoperative days. Acute dilatation and rupture of the stomach can happen if all the nasogastric tube holes are in the jejunum after gastric bypass. Erosion of the stomach by the hard end of the nasogastric tube has occurred when the tube was positioned in the upper stomach. This paper is dedicated to the prevention of death by early recognition and aggressive management of perforation and by prevention of perforations through careful attention to the details of these operations and early postoperative care.", "contents": "Risk reduction in gastric operations for obesity. Although nearly devoid of late complications, gastric operations for obesity have resulted in 4.7% early postoperative perforations. For patients over 39 years of age who perforated, the first 11 patients died and the last 9 survived. Perforations are equally common in upper stomach, anastomosis, and lower stomach. They have become more frequent with the 50 ml upper stomach volume and 12 mm stoma that are required to assure optimum weight control. Perforation is as common with gastroplasty as with gastric bypass. If it occurs, it is normally within the first ten postoperative days. Acute dilatation and rupture of the stomach can happen if all the nasogastric tube holes are in the jejunum after gastric bypass. Erosion of the stomach by the hard end of the nasogastric tube has occurred when the tube was positioned in the upper stomach. This paper is dedicated to the prevention of death by early recognition and aggressive management of perforation and by prevention of perforations through careful attention to the details of these operations and early postoperative care."}
{"id": "PMID:464688", "title": "Stapled cystograstrotomy. A method of treatment for pediatric pancreatic pseudocyst.", "content": "An uncommon complication of pancreatic inflammatory disease or pancreatic trauma in children is the development of a pseudocyst. The English literature records less than 100 cases in children and emphasizes that surgical intervention is the indicated form of treatment. The authors have had experience with nine pediatric patients suffering from pseudocysts in a seven year period. All except one were successfully treated by internal drainage; the other spontaneously resolved. Four had cystogastrostomy using a stapler. This method is discussed as far as indications, technique, advantages and complications.", "contents": "Stapled cystograstrotomy. A method of treatment for pediatric pancreatic pseudocyst. An uncommon complication of pancreatic inflammatory disease or pancreatic trauma in children is the development of a pseudocyst. The English literature records less than 100 cases in children and emphasizes that surgical intervention is the indicated form of treatment. The authors have had experience with nine pediatric patients suffering from pseudocysts in a seven year period. All except one were successfully treated by internal drainage; the other spontaneously resolved. Four had cystogastrostomy using a stapler. This method is discussed as far as indications, technique, advantages and complications."}
{"id": "PMID:464689", "title": "Evidence for a pyloro-cholecystic reflex for gallbladder contraction.", "content": "We studied the effect of graded antral distension on gallbladder contraction both when gastrin release was promoted (alkaline distension) and when gastrin release was blocked (acid distension) in five dogs provided with innervated antral pouch, chronic bile fistula and gastric fistula. Graded distension of the antrum caused graded gallbladder contraction as evidenced by bilirubin output even when gastrin release was completely suppressed. This nongastrin mechanism of gallbladder contraction is abolished by parenteral atropine and by transthoracic truncal vagotomy. These observations provide evidence for a cholinergic, pyloro-cholecystic reflex for gallbladder contraction that is dependent on intact long vagal pathways. Similar reflex mechanisms have been shown to be initiated by antral distension and to cause pancreatic enzyme secretion (pyloro-pancreatic reflex) or acid secretion from the oxyntic gland area of the stomach (pyloro-oxyntic reflex). It would appear, therefore, that the antrum plays a central role in the integration of upper gastrointestinal function not only through the hormone gastrin but also through neural reflex mechanisms.", "contents": "Evidence for a pyloro-cholecystic reflex for gallbladder contraction. We studied the effect of graded antral distension on gallbladder contraction both when gastrin release was promoted (alkaline distension) and when gastrin release was blocked (acid distension) in five dogs provided with innervated antral pouch, chronic bile fistula and gastric fistula. Graded distension of the antrum caused graded gallbladder contraction as evidenced by bilirubin output even when gastrin release was completely suppressed. This nongastrin mechanism of gallbladder contraction is abolished by parenteral atropine and by transthoracic truncal vagotomy. These observations provide evidence for a cholinergic, pyloro-cholecystic reflex for gallbladder contraction that is dependent on intact long vagal pathways. Similar reflex mechanisms have been shown to be initiated by antral distension and to cause pancreatic enzyme secretion (pyloro-pancreatic reflex) or acid secretion from the oxyntic gland area of the stomach (pyloro-oxyntic reflex). It would appear, therefore, that the antrum plays a central role in the integration of upper gastrointestinal function not only through the hormone gastrin but also through neural reflex mechanisms."}
{"id": "PMID:464690", "title": "Common bile duct and Oddi sphincter pressure before and after endoscopic papillotomy in patients with common bile duct stones.", "content": "Pressures in the Oddi sphincter and common bile duct were determined before, immediately after and several weeks after endoscopic papillotomy. Fifteen patients were included in the study. Significant decreases in Oddi sphincter and common bile duct pressures were found and the decrease in Oddi sphincter pressure was to some extent dependent upon the length of the papillotomy.", "contents": "Common bile duct and Oddi sphincter pressure before and after endoscopic papillotomy in patients with common bile duct stones. Pressures in the Oddi sphincter and common bile duct were determined before, immediately after and several weeks after endoscopic papillotomy. Fifteen patients were included in the study. Significant decreases in Oddi sphincter and common bile duct pressures were found and the decrease in Oddi sphincter pressure was to some extent dependent upon the length of the papillotomy."}
{"id": "PMID:464691", "title": "Primary malignant duodenal tumors.", "content": "Fourteen patients with primary malignant duodenal tumors are studied. Twelve patients had adenocarcinomas and two patients had malignant lymphomas. Preoperative diagnostic procedures, such as radiographic study of duodenum with hypotonic duodenography, complete duodenoscopy with biopsy and artertiographic studies are discussed. Early diagnosis is the key for curative surgical treatment since these tumors seem to disseminate rather late. In patients with primary duodenal carcinoma the resectability rate was 66.7%-seven Whipple resections and one segmental resection. Only one patient in our series died after a Whipple resection. There was no mortality after other procedures. The five year survival rate was 14.2%. There is a definite correlation between regional lymph node involvement and survival time. The mean survival period after Whipple resection without lymph node invasion is 56.5 months in our series. The survival period after Whipple resection for adenocarcinoma with regional lymph node invasion (6 months) is identical to the survival after palliative internal derivations for duodenal adenocarcinoma (5.8 months). It is concluded that a positive peroperative frozen section of a regional lymph node should exclude resective procedures (except in patients who hemorrhage) because they have a higher mortality rate as derivative procedures. Fifty per cent of the patients treated for malignant duodenal lymphoma is tumor free two years after a radical surgical therapy combined with chemotherapeutical treatment from the early postoperative period.", "contents": "Primary malignant duodenal tumors. Fourteen patients with primary malignant duodenal tumors are studied. Twelve patients had adenocarcinomas and two patients had malignant lymphomas. Preoperative diagnostic procedures, such as radiographic study of duodenum with hypotonic duodenography, complete duodenoscopy with biopsy and artertiographic studies are discussed. Early diagnosis is the key for curative surgical treatment since these tumors seem to disseminate rather late. In patients with primary duodenal carcinoma the resectability rate was 66.7%-seven Whipple resections and one segmental resection. Only one patient in our series died after a Whipple resection. There was no mortality after other procedures. The five year survival rate was 14.2%. There is a definite correlation between regional lymph node involvement and survival time. The mean survival period after Whipple resection without lymph node invasion is 56.5 months in our series. The survival period after Whipple resection for adenocarcinoma with regional lymph node invasion (6 months) is identical to the survival after palliative internal derivations for duodenal adenocarcinoma (5.8 months). It is concluded that a positive peroperative frozen section of a regional lymph node should exclude resective procedures (except in patients who hemorrhage) because they have a higher mortality rate as derivative procedures. Fifty per cent of the patients treated for malignant duodenal lymphoma is tumor free two years after a radical surgical therapy combined with chemotherapeutical treatment from the early postoperative period."}
{"id": "PMID:464692", "title": "A long-term follow-up of patients resected for benign esophageal stricture using the Inkwell esophagogastrostomy.", "content": "A total of 45 patients have undergone an Inkwell esophagogastrostomy after resection of a benign esophageal stricture. Nineteen patients were available for a long-term follow-up study, showing evidence of gastroesophageal reflux in 16 patients. Due to the high incidence of reflux and subsequent morphologic changes, there was a not negligible late disability. The Inkwell esophagogastrostomy can no longer be recommended in the treatment of an underlying reflux-induced lesion, strictures included.", "contents": "A long-term follow-up of patients resected for benign esophageal stricture using the Inkwell esophagogastrostomy. A total of 45 patients have undergone an Inkwell esophagogastrostomy after resection of a benign esophageal stricture. Nineteen patients were available for a long-term follow-up study, showing evidence of gastroesophageal reflux in 16 patients. Due to the high incidence of reflux and subsequent morphologic changes, there was a not negligible late disability. The Inkwell esophagogastrostomy can no longer be recommended in the treatment of an underlying reflux-induced lesion, strictures included."}
{"id": "PMID:464693", "title": "Effects of methylprednisolone on experimental pulmonary injury.", "content": "We studied the effects of methylprednisolone on pulmonary function of unanesthetized dogs with oleic acid induced pulmonary edema observed over a four day period. Methylprednisolone (30 mg/kg) was administered to 11 dogs three and 24 hours after pulmonary injury. Eleven animals were untreated after pulmonary injury and served as controls. There was no difference between the two groups until 72 hours after injury, when the venous admixture of the steroid treated animals was 11 +/- 3% (SD) compared to 22 +/- 8% (p less than 0.001) in the untreated with respective PaO2 values of 76 +/- 6 torr and 64 +/- 8 torr (p less than 0.001). Light microscopic examination of the lungs 96 hours after injury revealed a marked proliferation of Type II pneumocytes in the methylprednisolone treated animals. We conclude that, in the oleic acid or fat embolism model of pulmonary injury, methylprednisolone significantly increases resolution of the pulmonary injury presumably by stimulation of active proliferation and maturation of Type II pneumocytes.", "contents": "Effects of methylprednisolone on experimental pulmonary injury. We studied the effects of methylprednisolone on pulmonary function of unanesthetized dogs with oleic acid induced pulmonary edema observed over a four day period. Methylprednisolone (30 mg/kg) was administered to 11 dogs three and 24 hours after pulmonary injury. Eleven animals were untreated after pulmonary injury and served as controls. There was no difference between the two groups until 72 hours after injury, when the venous admixture of the steroid treated animals was 11 +/- 3% (SD) compared to 22 +/- 8% (p less than 0.001) in the untreated with respective PaO2 values of 76 +/- 6 torr and 64 +/- 8 torr (p less than 0.001). Light microscopic examination of the lungs 96 hours after injury revealed a marked proliferation of Type II pneumocytes in the methylprednisolone treated animals. We conclude that, in the oleic acid or fat embolism model of pulmonary injury, methylprednisolone significantly increases resolution of the pulmonary injury presumably by stimulation of active proliferation and maturation of Type II pneumocytes."}
{"id": "PMID:464694", "title": "Revascularization of the ischemic canine hindlimb by arteriovenous reversal.", "content": "Arteriovenous reversal (AVR) for revascularization of ischemic tissues has previously failed to meet theoretical, experimental, and clinical expectations despite recurrent trials. The efficacy of a new staged approach to AVR was tested against a canine ischemic limb preparation in which global ligation of ipsilateral collaterals inevitably led to limb gangrene. In 12 animals the complications of direct, single-stage end-to-end femoral AVR, inevitably accompanied by the development of extreme edema, were demonstrated. However, when the ischemic preparation was accompanied by a staged AVR, in which an initial end-artery-to-side-vein arteriovenous fistula was converted 1 week later to AVR by ligation of the central venous limb, 20 of 20 animals survived, and 19 of 20 were ambulatory long-term survivors with only mild edema. Serial angiograms at 1 week, 1 month, and 4 months demonstrated patency rates of 100, 84, and 63%, respectively. Histologic examination of animals electively killed from 4 to 24 months showed normal skeletal muscle histology, venous intimal thickening, and mild edema. In the acutely ischemic canine hind limb, a staged AVR can provide both viability and function with only mild edema formation.", "contents": "Revascularization of the ischemic canine hindlimb by arteriovenous reversal. Arteriovenous reversal (AVR) for revascularization of ischemic tissues has previously failed to meet theoretical, experimental, and clinical expectations despite recurrent trials. The efficacy of a new staged approach to AVR was tested against a canine ischemic limb preparation in which global ligation of ipsilateral collaterals inevitably led to limb gangrene. In 12 animals the complications of direct, single-stage end-to-end femoral AVR, inevitably accompanied by the development of extreme edema, were demonstrated. However, when the ischemic preparation was accompanied by a staged AVR, in which an initial end-artery-to-side-vein arteriovenous fistula was converted 1 week later to AVR by ligation of the central venous limb, 20 of 20 animals survived, and 19 of 20 were ambulatory long-term survivors with only mild edema. Serial angiograms at 1 week, 1 month, and 4 months demonstrated patency rates of 100, 84, and 63%, respectively. Histologic examination of animals electively killed from 4 to 24 months showed normal skeletal muscle histology, venous intimal thickening, and mild edema. In the acutely ischemic canine hind limb, a staged AVR can provide both viability and function with only mild edema formation."}
{"id": "PMID:464695", "title": "Minor hemobilia. Clinical significance and pathophysiological background.", "content": "Profuse hemorrhage into the biliary tract--major hemobilia,--is an alarming condition which attracts much attention. Minor hemobilia, often caused by gallstones or operative injury, is much more frequent, yet often neglected. Clinical observations indicate that minor hemobilia is not always an innocent condition with the blood remaining fluid and unobtrusively flowing into the intestine. Examples are given where clots from occult hemobilia have caused diagnostic errors or obstructed the bile flow, thus imitating gallstones for which they may be mistaken. Experiments have been performed to elucidate the pathophysiology of this clot formation: 1) A model of the biliary tract was constructed with bile flowing through it. When blood was injected forcefully to simulate a major hemorrhage, mixed clots of blood and bile were formed. When introduced gently, as in minor hemobilia, the blood flowed immiscibly to the lowest level where it formed a clot of pure blood. The clots dissolved under the influence of flowing bile, but remained intact when protected from the flow. 2) Mixed clots were mushy and dissolved spontaneously, while pure clots remained solid and stable. When clots containing increasing amounts of bile were incubated, increasing amounts of cleavage products of fibrinogen and fibrin were formed. 3) Blood clots were produced in gallbladders of 33 dogs. Pure clots remained solid and floating while mixed clots formed a mushy layer, strongly adherent to the mucosa. Both kinds of clots disappeared after two-four weeks, but remained if bile flow was diverted. These findings explain why under certain circumstances minor hemobilia acquires clinical significance by forming clots that may obstruct the flow or cause diagnostic errors. Successful dissolution of \"retained stones\" may occasionally have the simple explanation that blood clots, mistaken for calculi, have been fibrinolysed. Consequently, in biliary obstruction or when defects are found on cholangiography, the possibility of blood clots in the ducts should be considered even in the absence of overt gastrointestinal hemorrhage.", "contents": "Minor hemobilia. Clinical significance and pathophysiological background. Profuse hemorrhage into the biliary tract--major hemobilia,--is an alarming condition which attracts much attention. Minor hemobilia, often caused by gallstones or operative injury, is much more frequent, yet often neglected. Clinical observations indicate that minor hemobilia is not always an innocent condition with the blood remaining fluid and unobtrusively flowing into the intestine. Examples are given where clots from occult hemobilia have caused diagnostic errors or obstructed the bile flow, thus imitating gallstones for which they may be mistaken. Experiments have been performed to elucidate the pathophysiology of this clot formation: 1) A model of the biliary tract was constructed with bile flowing through it. When blood was injected forcefully to simulate a major hemorrhage, mixed clots of blood and bile were formed. When introduced gently, as in minor hemobilia, the blood flowed immiscibly to the lowest level where it formed a clot of pure blood. The clots dissolved under the influence of flowing bile, but remained intact when protected from the flow. 2) Mixed clots were mushy and dissolved spontaneously, while pure clots remained solid and stable. When clots containing increasing amounts of bile were incubated, increasing amounts of cleavage products of fibrinogen and fibrin were formed. 3) Blood clots were produced in gallbladders of 33 dogs. Pure clots remained solid and floating while mixed clots formed a mushy layer, strongly adherent to the mucosa. Both kinds of clots disappeared after two-four weeks, but remained if bile flow was diverted. These findings explain why under certain circumstances minor hemobilia acquires clinical significance by forming clots that may obstruct the flow or cause diagnostic errors. Successful dissolution of \"retained stones\" may occasionally have the simple explanation that blood clots, mistaken for calculi, have been fibrinolysed. Consequently, in biliary obstruction or when defects are found on cholangiography, the possibility of blood clots in the ducts should be considered even in the absence of overt gastrointestinal hemorrhage."}
{"id": "PMID:464699", "title": "Survival of patients with aortic valve endocarditis. The prognostic implications of the echocardiogram.", "content": "The clinical and prognostic implications of the echocardiogram were assessed in 32 patients with clinical aortic valve endocarditis. Seventeen patients had an echocardiographic diagnosis of a vegetation, a flail cusp, or a root abscess (group 1). Ten group 1 patients had embolic episodes. Eight group 1 patients were treated surgically and all survived, while nine group 1 patients were treated medically and two survived. Eleven patients had echocardiographic aortic valve sclerosis (group 2). One group 2 patient had an embolic episode. Six group 2 patients were treated surgically and four survived, while five group 2 patients were treated medically and three survived. Four patients had normal aortic valve echograms (group 3). One group 3 patient had an embolic episode. All group 3 patients were treated medically and survived.", "contents": "Survival of patients with aortic valve endocarditis. The prognostic implications of the echocardiogram. The clinical and prognostic implications of the echocardiogram were assessed in 32 patients with clinical aortic valve endocarditis. Seventeen patients had an echocardiographic diagnosis of a vegetation, a flail cusp, or a root abscess (group 1). Ten group 1 patients had embolic episodes. Eight group 1 patients were treated surgically and all survived, while nine group 1 patients were treated medically and two survived. Eleven patients had echocardiographic aortic valve sclerosis (group 2). One group 2 patient had an embolic episode. Six group 2 patients were treated surgically and four survived, while five group 2 patients were treated medically and three survived. Four patients had normal aortic valve echograms (group 3). One group 3 patient had an embolic episode. All group 3 patients were treated medically and survived."}
{"id": "PMID:464700", "title": "Incidence and outcome of the respiratory distress syndrome in gram-negative sepsis.", "content": "Twenty cases of the adult respiratory distress syndrome (ARDS) in 86 cases of Gram-negative bacteremia were identified. Features of Gram-negative bacteremia associated with the development of ARDS included a history of cardiac disease, hypotension, and thrombocytopenia. The mortality of patients with and without ARDS was 90% and 55%, respectively. The association of hypotension and thrombocytopenia with the subsequent development of ARDS suggests the possible role of endotoxin-induced coagulopathy in this complication.", "contents": "Incidence and outcome of the respiratory distress syndrome in gram-negative sepsis. Twenty cases of the adult respiratory distress syndrome (ARDS) in 86 cases of Gram-negative bacteremia were identified. Features of Gram-negative bacteremia associated with the development of ARDS included a history of cardiac disease, hypotension, and thrombocytopenia. The mortality of patients with and without ARDS was 90% and 55%, respectively. The association of hypotension and thrombocytopenia with the subsequent development of ARDS suggests the possible role of endotoxin-induced coagulopathy in this complication."}
{"id": "PMID:464701", "title": "Learning style in a primary care internal medicine residency program.", "content": "Individual differences in learning style should be one factor that is considered in medical education program planning and instruction, and this can be accomplished by using Kolb's learning style inventory (LSI) in a primary care internal medicine residency program. Case accounts are presented to suggest how the LSI can be used to enrich teaching-learning encounters. To our knowledge, these case accounts are the first reports of an important use of knowledge about learning style, obtained through instruments such as the LSI. Other educational applications are suggested with respect to evaluation formats and choice of instructional methods.", "contents": "Learning style in a primary care internal medicine residency program. Individual differences in learning style should be one factor that is considered in medical education program planning and instruction, and this can be accomplished by using Kolb's learning style inventory (LSI) in a primary care internal medicine residency program. Case accounts are presented to suggest how the LSI can be used to enrich teaching-learning encounters. To our knowledge, these case accounts are the first reports of an important use of knowledge about learning style, obtained through instruments such as the LSI. Other educational applications are suggested with respect to evaluation formats and choice of instructional methods."}
{"id": "PMID:464702", "title": "Effect of parathyroidectomy on anemia in chronic renal failure.", "content": "Fourteen patients with chronic renal failure underwent parathyroidectomy. Postoperatively, seven patients exhibited a rise in hematocrit reading, but seven others did not. Responders had more severe bone disease and lower initial hematocrit values than did nonresponders. Marrow fibrosis was slightly more prominent in responders. Current concepts of marrow erythropoietic inhibition in renal failure suggest a toxic serum factor as the cause. This report fails to support parathyroid hormone as the toxic agent directly responsible for marrow inhibition. Rather, parathyroid hormone may contribute to anemia in renal failure by causing marrow fibrosis, a process sometimes reversible by successful therapy of hyperparathyroidism.", "contents": "Effect of parathyroidectomy on anemia in chronic renal failure. Fourteen patients with chronic renal failure underwent parathyroidectomy. Postoperatively, seven patients exhibited a rise in hematocrit reading, but seven others did not. Responders had more severe bone disease and lower initial hematocrit values than did nonresponders. Marrow fibrosis was slightly more prominent in responders. Current concepts of marrow erythropoietic inhibition in renal failure suggest a toxic serum factor as the cause. This report fails to support parathyroid hormone as the toxic agent directly responsible for marrow inhibition. Rather, parathyroid hormone may contribute to anemia in renal failure by causing marrow fibrosis, a process sometimes reversible by successful therapy of hyperparathyroidism."}
{"id": "PMID:464703", "title": "Epidemiology of infectious syphilis at a tertiary hospital.", "content": "In 19 of 20 patients in whom the correct diagnosis of primary or secondary syphilis was not obvious initially, a positive routine admission VDRL test result was the first indication of the correct diagnosis. A retrospective study during a one-month period to correlate hospital admissions by clinical service with required routine admission serologic tests for syphilis disclosed an overall compliance rate of only 37.1% (range, 94.6% to 8.0%). Of the 38 patients who did have a positive admission VDRL test result, 55.3% were falsely positive. The false-positive rate was slightly higher for weakly reactive titers than it was for higher titers. The routine serologic testing program uncovered 129 and 116 new cases of syphilis during 1976 and 1977, respectively. The decision to continue the routine admission VDRL screening program was made because the consequences of an incorrect diagnosis could be very great for an individual patient.", "contents": "Epidemiology of infectious syphilis at a tertiary hospital. In 19 of 20 patients in whom the correct diagnosis of primary or secondary syphilis was not obvious initially, a positive routine admission VDRL test result was the first indication of the correct diagnosis. A retrospective study during a one-month period to correlate hospital admissions by clinical service with required routine admission serologic tests for syphilis disclosed an overall compliance rate of only 37.1% (range, 94.6% to 8.0%). Of the 38 patients who did have a positive admission VDRL test result, 55.3% were falsely positive. The false-positive rate was slightly higher for weakly reactive titers than it was for higher titers. The routine serologic testing program uncovered 129 and 116 new cases of syphilis during 1976 and 1977, respectively. The decision to continue the routine admission VDRL screening program was made because the consequences of an incorrect diagnosis could be very great for an individual patient."}
{"id": "PMID:464704", "title": "Bone marrow necrosis and degeneration.", "content": "Bone marrow necrosis, regarded as a rare finding in specimens from living patients, has been associated with a poor prognosis in patients with serious hematologic diseases and metastatic carcinoma. Two patients with extensive idiopathic bone marrow necrosis and a relatively benign course of illness were found. Therefore, we examined 500 consecutive bone marrow biopsy specimens that were obtained in a university hospital complex. Review of this material showed evidence of necrosis and degenerative changes of variable severity in one third of the biopsy specimens. It was found with approximately the same incidence in patients who underwent bone marrow biopsy for either neoplastic or nonneoplastic disorders; an increased prevalence was not observed in the group of patients who had received chemotherapy. Based on these observations, we believe necrosis and degeneration of the bone marrow is a commonplace finding that is frequently overlooked in a wide variety of acute and chronic disorders, and that requires further investigation to determine its clinical importance.", "contents": "Bone marrow necrosis and degeneration. Bone marrow necrosis, regarded as a rare finding in specimens from living patients, has been associated with a poor prognosis in patients with serious hematologic diseases and metastatic carcinoma. Two patients with extensive idiopathic bone marrow necrosis and a relatively benign course of illness were found. Therefore, we examined 500 consecutive bone marrow biopsy specimens that were obtained in a university hospital complex. Review of this material showed evidence of necrosis and degenerative changes of variable severity in one third of the biopsy specimens. It was found with approximately the same incidence in patients who underwent bone marrow biopsy for either neoplastic or nonneoplastic disorders; an increased prevalence was not observed in the group of patients who had received chemotherapy. Based on these observations, we believe necrosis and degeneration of the bone marrow is a commonplace finding that is frequently overlooked in a wide variety of acute and chronic disorders, and that requires further investigation to determine its clinical importance."}
{"id": "PMID:464712", "title": "Survival with severe hypernatremia.", "content": "In a 74-year-old woman, excessive insensible water loss developed secondary to a period of hot, humid weather, associated with an underlying inability to obtain adequate water replacement. On admission to the hospital she was comatose, clinically dehydrated, and had laboratory values consistent with a water deficit of approximately 30% (9 L) of body water. Serum sodium concentration was 202 mEq/L. Serum osmolality was 430 mOsm/L. The patient remained comatose for seven days, during which time she was vigorously treated with fluids, and she gradually recovered. This case represents what we believe is a unique report in the literature of the severity of hypernatremia developing via this pathogenic mechanism and survival in an adult with this degree of disturbance of sodium and water homeostasis.", "contents": "Survival with severe hypernatremia. In a 74-year-old woman, excessive insensible water loss developed secondary to a period of hot, humid weather, associated with an underlying inability to obtain adequate water replacement. On admission to the hospital she was comatose, clinically dehydrated, and had laboratory values consistent with a water deficit of approximately 30% (9 L) of body water. Serum sodium concentration was 202 mEq/L. Serum osmolality was 430 mOsm/L. The patient remained comatose for seven days, during which time she was vigorously treated with fluids, and she gradually recovered. This case represents what we believe is a unique report in the literature of the severity of hypernatremia developing via this pathogenic mechanism and survival in an adult with this degree of disturbance of sodium and water homeostasis."}
{"id": "PMID:464713", "title": "Recurrence after a surgically induced remission.", "content": "In December 1976, an 18-year-old woman had symptoms typical of Cushing's syndrome. Laboratory evaluations and roentgenograms documented pituitary-dependent Cushing's disease in a patient with a pituitary microadenoma. In May 1977, she underwent transsphenoidal pituitary exploration. A 2-mm pituitary microadenoma was removed. The patient improved, and laboratory evaluation documented remission of the disease. In June 1978, she again complained of symptoms compatible with Cushing's disease. Laboratory evaluation confirmed a pituitary-dependent hypercortisonism. This case report marks the first recurrence of Cushing's disease in a patient previously cured by transsphenoidal resection of a pituitary tumor.", "contents": "Recurrence after a surgically induced remission. In December 1976, an 18-year-old woman had symptoms typical of Cushing's syndrome. Laboratory evaluations and roentgenograms documented pituitary-dependent Cushing's disease in a patient with a pituitary microadenoma. In May 1977, she underwent transsphenoidal pituitary exploration. A 2-mm pituitary microadenoma was removed. The patient improved, and laboratory evaluation documented remission of the disease. In June 1978, she again complained of symptoms compatible with Cushing's disease. Laboratory evaluation confirmed a pituitary-dependent hypercortisonism. This case report marks the first recurrence of Cushing's disease in a patient previously cured by transsphenoidal resection of a pituitary tumor."}
{"id": "PMID:464732", "title": "Complete degradation of carbohydrate to carbon dioxide and methane by syntrophic cultures of Acetobacterium woodii and Methanosarcina barkeri.", "content": "Methanosarcina barkeri (strain MS) grew and converted acetate to CO2 and methane after an adaption period of 20 days. Growth and metabolism were rapid with gas production being comparable to that of cells grown on H2 and CO2. After an intermediary growth cycle under a H2 and CO2 atmosphere acetate-adapted cells were capable of growth on acetate with formation of methane and CO2. When acetate-adapted Methanosarcina barkeri was co-cultured with Acetobacterium woodii on fructose or glucose as substrate, a complete conversion of the carbohydrate to gases (CO2 and CH4) was observed.", "contents": "Complete degradation of carbohydrate to carbon dioxide and methane by syntrophic cultures of Acetobacterium woodii and Methanosarcina barkeri. Methanosarcina barkeri (strain MS) grew and converted acetate to CO2 and methane after an adaption period of 20 days. Growth and metabolism were rapid with gas production being comparable to that of cells grown on H2 and CO2. After an intermediary growth cycle under a H2 and CO2 atmosphere acetate-adapted cells were capable of growth on acetate with formation of methane and CO2. When acetate-adapted Methanosarcina barkeri was co-cultured with Acetobacterium woodii on fructose or glucose as substrate, a complete conversion of the carbohydrate to gases (CO2 and CH4) was observed."}
{"id": "PMID:464738", "title": "Gender identity change in transsexuals. Follow-up and replications.", "content": "An apparently successful change in gender identity of an adolescent transsexual was reported several years ago. Gender-specific motor behavior, appropriate sex role social behavior, cognitive sexual activity, and finally sexual arousal patterns were defined, measured, and sequentially modified. In this report, a 6 1/2-year follow-up is presented and the application of a similar therapeutic package to two additional cases is described. In the first case, sweeping changes in gender identity, sex-role behavior, and sexual arousal patterns were observed. In the second of these cases, rigid feminine gender was given up, but the patient chose to retain homosexual arousal. Social adaptation was satisfactory. These findings and other recent developments point to the possibility of psychosocial intervention as an alternative to surgery in the treatment of transsexualism.", "contents": "Gender identity change in transsexuals. Follow-up and replications. An apparently successful change in gender identity of an adolescent transsexual was reported several years ago. Gender-specific motor behavior, appropriate sex role social behavior, cognitive sexual activity, and finally sexual arousal patterns were defined, measured, and sequentially modified. In this report, a 6 1/2-year follow-up is presented and the application of a similar therapeutic package to two additional cases is described. In the first case, sweeping changes in gender identity, sex-role behavior, and sexual arousal patterns were observed. In the second of these cases, rigid feminine gender was given up, but the patient chose to retain homosexual arousal. Social adaptation was satisfactory. These findings and other recent developments point to the possibility of psychosocial intervention as an alternative to surgery in the treatment of transsexualism."}
{"id": "PMID:464739", "title": "Sex reassignment. Follow-up.", "content": "Although medical interest in individuals adopting the dress and life-style of the opposite sex goes back to antiquity, surgical intervention is a product of the last 50 years. In the last 15 years, evaluation procedures and surgical techniques have been worked out. Extended evaluation, with a one- to two-year trial period prior to formal consideration of surgery, is accepted practice at reputable centers. Cosmetically satisfactory, and often functional, genitalia can be constructed. Less clear-cut however, are the characteristics of the applicants for sex reassignment, the natural history of the compulsion toward surgery, and surgery's long-term effects. The characteristics of 50 applicants for sex reassignment, both operated and unoperated, are reported in terms of such indices as job, education, marital, and domiciliary stability. Outcome are reviewed. The results of long-term follow up data are discussed in terms of the adjustments of operated and unoperated patients.", "contents": "Sex reassignment. Follow-up. Although medical interest in individuals adopting the dress and life-style of the opposite sex goes back to antiquity, surgical intervention is a product of the last 50 years. In the last 15 years, evaluation procedures and surgical techniques have been worked out. Extended evaluation, with a one- to two-year trial period prior to formal consideration of surgery, is accepted practice at reputable centers. Cosmetically satisfactory, and often functional, genitalia can be constructed. Less clear-cut however, are the characteristics of the applicants for sex reassignment, the natural history of the compulsion toward surgery, and surgery's long-term effects. The characteristics of 50 applicants for sex reassignment, both operated and unoperated, are reported in terms of such indices as job, education, marital, and domiciliary stability. Outcome are reviewed. The results of long-term follow up data are discussed in terms of the adjustments of operated and unoperated patients."}
{"id": "PMID:464740", "title": "Centerfold. An essay on excitement.", "content": "A woman who poses for soft-core pornography reports that she has never felt she belonged to her body, that she is and wants only to be an erotic product manufactured by a team of specialists for the use of a viewing audience of males, that since age 5 she has been a nude dancer, and that she has no other material existence except in this form. She is, then, a fetish. Her success illustrates the hypothesis that erotic daydreams in pronography represent fantasies of revenge in which the consumer imagines he is degrading--dehumanizing--women.", "contents": "Centerfold. An essay on excitement. A woman who poses for soft-core pornography reports that she has never felt she belonged to her body, that she is and wants only to be an erotic product manufactured by a team of specialists for the use of a viewing audience of males, that since age 5 she has been a nude dancer, and that she has no other material existence except in this form. She is, then, a fetish. Her success illustrates the hypothesis that erotic daydreams in pronography represent fantasies of revenge in which the consumer imagines he is degrading--dehumanizing--women."}
{"id": "PMID:464743", "title": "Nonverbal behavior and thought processing.", "content": "Generally, clinicians assessing mental status interpret patients' nonverbal behavior in terms of its discharge, expressive, interactional, or neurological properties. The present study addresses the conceptualization of hand movement behavior as related to central cognitive processes, and attempts to identify encoding-related motor behavior by comparing the hand movements of subordinate bilingual subjects in situations of dominant- and nondominant-language and low- and high-imagery verbalization. Compared to the parallel dominant-language situation, subjects verbalizing in their nondominant language produced more speech-primacy and groping hand movements. Also, in comparison with the verbalization about a high-imagery topic, when the subjects encoded a low-imagery topic they displayed more pointing movements. Unless they are aware of these movements, clinicians evaluating bilingual patients may interpret an increase in encoding-related motor activity as reflecting psychopathology. Apart from this psychodiagnostic significance, hypothetical implications of these findings for the study of aphasia and information processing mechanisms are discussed.", "contents": "Nonverbal behavior and thought processing. Generally, clinicians assessing mental status interpret patients' nonverbal behavior in terms of its discharge, expressive, interactional, or neurological properties. The present study addresses the conceptualization of hand movement behavior as related to central cognitive processes, and attempts to identify encoding-related motor behavior by comparing the hand movements of subordinate bilingual subjects in situations of dominant- and nondominant-language and low- and high-imagery verbalization. Compared to the parallel dominant-language situation, subjects verbalizing in their nondominant language produced more speech-primacy and groping hand movements. Also, in comparison with the verbalization about a high-imagery topic, when the subjects encoded a low-imagery topic they displayed more pointing movements. Unless they are aware of these movements, clinicians evaluating bilingual patients may interpret an increase in encoding-related motor activity as reflecting psychopathology. Apart from this psychodiagnostic significance, hypothetical implications of these findings for the study of aphasia and information processing mechanisms are discussed."}
{"id": "PMID:464744", "title": "Cerebral lateralization and personality style.", "content": "Hysterical and obsessive-compulsive style subjects, as determined by a modified Rorschach test and the Wechslar Adult Intelligence Scale Comprehension subtest, were questioned in a face-to-face format where lateral eye movements were surreptitiously observed. Only right-handed subjects were used. It has been argued that lateral eye movements index immediate hemispheric activation after questions--the first such eye movement being a direction opposite to the putatively activated hemisphere. There was significantly more left-looking among the hysterical subjects than among the obsessive-compulsive style subjects.", "contents": "Cerebral lateralization and personality style. Hysterical and obsessive-compulsive style subjects, as determined by a modified Rorschach test and the Wechslar Adult Intelligence Scale Comprehension subtest, were questioned in a face-to-face format where lateral eye movements were surreptitiously observed. Only right-handed subjects were used. It has been argued that lateral eye movements index immediate hemispheric activation after questions--the first such eye movement being a direction opposite to the putatively activated hemisphere. There was significantly more left-looking among the hysterical subjects than among the obsessive-compulsive style subjects."}
{"id": "PMID:464745", "title": "Adaptational problems of Vietnamese refugees. I. Health and mental health status.", "content": "The forced migratory influx of Vietnamese to the United States has raised questions regarding the resettlement process, the effect of culture shock, the refugees' coping behavior and adaptabilities, and their health and mental health status. We report the two-year results of ongoing research on the Vietnamese refugees based on the use of the Cornell Medical Index (CMI). The responses on the CMI on the first (1975) and second (1976) administrations indicate a high and continuing level of physical and mental dysfunction. The second administration also revealed significant shifts in dysfunctions as well as exposing factors that related to these dysfunctions, ie, age/sex interactions, marital status, family groupings, and public assistance. The follow-up CMI also showed an increase in anger and hostility with concomitant reductions in feelings of inadequacy.", "contents": "Adaptational problems of Vietnamese refugees. I. Health and mental health status. The forced migratory influx of Vietnamese to the United States has raised questions regarding the resettlement process, the effect of culture shock, the refugees' coping behavior and adaptabilities, and their health and mental health status. We report the two-year results of ongoing research on the Vietnamese refugees based on the use of the Cornell Medical Index (CMI). The responses on the CMI on the first (1975) and second (1976) administrations indicate a high and continuing level of physical and mental dysfunction. The second administration also revealed significant shifts in dysfunctions as well as exposing factors that related to these dysfunctions, ie, age/sex interactions, marital status, family groupings, and public assistance. The follow-up CMI also showed an increase in anger and hostility with concomitant reductions in feelings of inadequacy."}
{"id": "PMID:464746", "title": "Multimodality treatment. A one-year follow-up of 84 hyperactive boys.", "content": "This study reports findings at the end of the first year of a three-year prospective study of 84 hyperactive boys. A multidimensional approach to evaluation and an individualized multimodality treatment plan commensurate with each child's disabilities was used. Treatment plans were implemented by members of the research staff working together as a coordinated therapeutic team. Measures of the child's behavior at home and at school, academic performance, delinquent behavior, and emotional adjustment were obtained initially and at one year. Results suggest that the combination of a clinically useful medication with appropriate psychological treatments simultaneously directed to each of the child's many disabilities is associated with an unexpectedly good outcome. Whether this will continue to be true when these children are followed up over a longer period of time awaits further investigation.", "contents": "Multimodality treatment. A one-year follow-up of 84 hyperactive boys. This study reports findings at the end of the first year of a three-year prospective study of 84 hyperactive boys. A multidimensional approach to evaluation and an individualized multimodality treatment plan commensurate with each child's disabilities was used. Treatment plans were implemented by members of the research staff working together as a coordinated therapeutic team. Measures of the child's behavior at home and at school, academic performance, delinquent behavior, and emotional adjustment were obtained initially and at one year. Results suggest that the combination of a clinically useful medication with appropriate psychological treatments simultaneously directed to each of the child's many disabilities is associated with an unexpectedly good outcome. Whether this will continue to be true when these children are followed up over a longer period of time awaits further investigation."}
{"id": "PMID:464747", "title": "The laminar and size distribution of commissural efferent neurons in the cat visual cortex.", "content": "The laminar and size distribution of commissural efferent neurons were studied in the cat visual cortex including area 17, 18, 19 and the lateral suprasylvian cortex (the lateral wall of the middle suprasylvian gyrus) by means of retrograde transport of horseradish peroxidase. Single or multiple injections of the enzyme were made unilaterally along the medial part of the lateral gyrus (the adjoining parts of area 17 and 18) or along the lateral wall of the middle suprasylvian gyrus. In the contralateral cerebral cortex (area 17, 18, 19 and lateral suprasylvian cortex) the majority of peroxidase labeled commissural efferent neurons was identified in layer III with a smaller population in other layers except layer I. The size distribution of labeled cells of layer III showed that all sizes of neurons contribute to interhemispheric connections, ranging from small cells which are comparable to those predominating in layer III of the acommissural medial area 17 to the characteristic large pyramides of layer III at the area 17/18 boundary. There was a certain difference in the pattern of size distribution of commissural efferent neurons of layer III between area 17, 18, 19 and the lateral suprasylvian cortex. Labeled neurons in area 17, 18, 19 showed a dominant peak in the histograms of cell size, while in the lateral suprasylvian cortex they were more evenly distributed in a wide size range.", "contents": "The laminar and size distribution of commissural efferent neurons in the cat visual cortex. The laminar and size distribution of commissural efferent neurons were studied in the cat visual cortex including area 17, 18, 19 and the lateral suprasylvian cortex (the lateral wall of the middle suprasylvian gyrus) by means of retrograde transport of horseradish peroxidase. Single or multiple injections of the enzyme were made unilaterally along the medial part of the lateral gyrus (the adjoining parts of area 17 and 18) or along the lateral wall of the middle suprasylvian gyrus. In the contralateral cerebral cortex (area 17, 18, 19 and lateral suprasylvian cortex) the majority of peroxidase labeled commissural efferent neurons was identified in layer III with a smaller population in other layers except layer I. The size distribution of labeled cells of layer III showed that all sizes of neurons contribute to interhemispheric connections, ranging from small cells which are comparable to those predominating in layer III of the acommissural medial area 17 to the characteristic large pyramides of layer III at the area 17/18 boundary. There was a certain difference in the pattern of size distribution of commissural efferent neurons of layer III between area 17, 18, 19 and the lateral suprasylvian cortex. Labeled neurons in area 17, 18, 19 showed a dominant peak in the histograms of cell size, while in the lateral suprasylvian cortex they were more evenly distributed in a wide size range."}
{"id": "PMID:464748", "title": "The Merkel cell in the labial ridge epidermis of the anuran tadpole. II. Electron microscope observations on the appearance and differentiation of the Merkel cell.", "content": "The appearance and cytodifferentiation of the Merkel cell were studied electron microscopically using the developing labial ridges of Rana japonica. The most primitive Merkel cell was identified with specific cored granules with an average diameter of about 90 nm. Such a primitive Merkel cell was found in the basal layer of the primitive labial ridge epithelium. As the primitive labial ridge continued to mature, the primitive Merkel cell accumulated an increased number of the specific granules, developed digital shaped cytoplasmic processes and came into contact with nerve fibers. When the labial ridge was sufficiently developed the cell showed typical morphological features of the mature Merkel cell except for a small accumulation of tonofilament-like structures and glycogen particles. It was also observed that the position of the Merkel cell in the epithelium changed from the basel layer to the third basal layer during development. On the other hand, it was difficult to find the Merkel cell in the mesenchymal layer of the developing labial ridges. From the results, it was suggested that the cytodifferentiation of the Merkel cell in the labial ridge of an anuran tadpole occurs within the epithelium.", "contents": "The Merkel cell in the labial ridge epidermis of the anuran tadpole. II. Electron microscope observations on the appearance and differentiation of the Merkel cell. The appearance and cytodifferentiation of the Merkel cell were studied electron microscopically using the developing labial ridges of Rana japonica. The most primitive Merkel cell was identified with specific cored granules with an average diameter of about 90 nm. Such a primitive Merkel cell was found in the basal layer of the primitive labial ridge epithelium. As the primitive labial ridge continued to mature, the primitive Merkel cell accumulated an increased number of the specific granules, developed digital shaped cytoplasmic processes and came into contact with nerve fibers. When the labial ridge was sufficiently developed the cell showed typical morphological features of the mature Merkel cell except for a small accumulation of tonofilament-like structures and glycogen particles. It was also observed that the position of the Merkel cell in the epithelium changed from the basel layer to the third basal layer during development. On the other hand, it was difficult to find the Merkel cell in the mesenchymal layer of the developing labial ridges. From the results, it was suggested that the cytodifferentiation of the Merkel cell in the labial ridge of an anuran tadpole occurs within the epithelium."}
{"id": "PMID:464749", "title": "Ultrastructure of intercellular junctions in the rat exocrine pancreas stimulated by pancreozymin.", "content": "Intercellular junctions in the exocrine pancreas, through which pancreatic enzymes may leak out from the lumen into the intercellular spaces, were studied in the rat by means of electron microscopy using tannic acid as a tracer and freeze-fracture methods as well as conventional thin sections. In thin sections and freeze-fracture replicas from the pancreas stimulated or not stimulated by exogenous pancreozymin, tight junctions were located between adjacent cells of the acinus or duct at the level just below the lumen. Observation of freeze-fracture replicas revealed that the number of strands or furrows in the tight junction did not differ significantly between the two groups but the width of the tight junction area was narrower and the distance between neighboring strands or furrows in the junction was smaller in the stimulated pancreas than in the control pancreas. Tannic acid, the molecular weight of which is smaller than that of pancreatic enzymes, could not pass beyond the tight junction area into the lumen. This study suggests that the pancreatic enzymes can not leak out from the glandular lumen into the intercellular spaces by dissociation of intercellular junctions even if the pancreas is stimulated by a high concentration of pancreozymin.", "contents": "Ultrastructure of intercellular junctions in the rat exocrine pancreas stimulated by pancreozymin. Intercellular junctions in the exocrine pancreas, through which pancreatic enzymes may leak out from the lumen into the intercellular spaces, were studied in the rat by means of electron microscopy using tannic acid as a tracer and freeze-fracture methods as well as conventional thin sections. In thin sections and freeze-fracture replicas from the pancreas stimulated or not stimulated by exogenous pancreozymin, tight junctions were located between adjacent cells of the acinus or duct at the level just below the lumen. Observation of freeze-fracture replicas revealed that the number of strands or furrows in the tight junction did not differ significantly between the two groups but the width of the tight junction area was narrower and the distance between neighboring strands or furrows in the junction was smaller in the stimulated pancreas than in the control pancreas. Tannic acid, the molecular weight of which is smaller than that of pancreatic enzymes, could not pass beyond the tight junction area into the lumen. This study suggests that the pancreatic enzymes can not leak out from the glandular lumen into the intercellular spaces by dissociation of intercellular junctions even if the pancreas is stimulated by a high concentration of pancreozymin."}
{"id": "PMID:464750", "title": "Fluorescence microscopic and enzyme histochemical studies of the innervation of the human spleen.", "content": "The innervation of the human spleen was investigated by the fluorescence method for adrenergic nerve fibers (52 cases) and the enzyme histochemical method for cholinergic nerve fibers (27 cases). The spleens examined were removed by laparotomy chiefly for gastric cancer. Adrenergic nerve fibers were demonstrated at the medioadventitial junction and the media in the trabecular arteries, whereas they occurred at the medioadventitial junction in the central and penicillar arteries. They were not found in the trabecular and pulp veins and venules, sheathed capillaries, avascular trabeculae, white and red pulp, and capsule. Nerves containing only a few adrenergic fibers and nerves without adrenergic fibers occurred close to the trabecular arteries. It was also demonstrated that nerves close to the trabecular arteries contain cholinergic fibers. The present study first verifies histochemically that the human spleen is innervated doubly by adrenergic and cholinergic nerve fibers.", "contents": "Fluorescence microscopic and enzyme histochemical studies of the innervation of the human spleen. The innervation of the human spleen was investigated by the fluorescence method for adrenergic nerve fibers (52 cases) and the enzyme histochemical method for cholinergic nerve fibers (27 cases). The spleens examined were removed by laparotomy chiefly for gastric cancer. Adrenergic nerve fibers were demonstrated at the medioadventitial junction and the media in the trabecular arteries, whereas they occurred at the medioadventitial junction in the central and penicillar arteries. They were not found in the trabecular and pulp veins and venules, sheathed capillaries, avascular trabeculae, white and red pulp, and capsule. Nerves containing only a few adrenergic fibers and nerves without adrenergic fibers occurred close to the trabecular arteries. It was also demonstrated that nerves close to the trabecular arteries contain cholinergic fibers. The present study first verifies histochemically that the human spleen is innervated doubly by adrenergic and cholinergic nerve fibers."}
{"id": "PMID:464751", "title": "Pairing of spermatozoa in the epididymis of the opossum (Didelphis virginiana): a scanning electron microscopic study.", "content": "Spermatozoa of the opossum, Didelphis virginiana, were taken from three different regions of the epididymis and examined by scanning electron microscopy, phase-contrast and dark-field microscopy. It has been demonstrated that marked morphological changes occur in spermatozoa during their passage through the epididymis. In the first segment or head of the epididymis, spermatozoa are non-motile and the long axis of their nuclei is perpendicular to that of the tail. The nucleus is V-shaped with a thick and a thin arm. At the junction of the two arms. At the junction of the two arms, where the arms join, the articular fossa receives the capitulum of the connecting piece which attaches the head of the spermatozoon to the tail. Spermatozoa from the central region of the epididymis show a re-orientation of the nuclei which now lie parallel to the long axis of the tail. Both paired and unpaired spermatozoa are found and show an increase in motility. In the caudal region of the epididymis all spermatozoa are paired. Pairing occurs as a result of the close apposition of adjacent cell membranes covering the acrosomes of the larger arms.", "contents": "Pairing of spermatozoa in the epididymis of the opossum (Didelphis virginiana): a scanning electron microscopic study. Spermatozoa of the opossum, Didelphis virginiana, were taken from three different regions of the epididymis and examined by scanning electron microscopy, phase-contrast and dark-field microscopy. It has been demonstrated that marked morphological changes occur in spermatozoa during their passage through the epididymis. In the first segment or head of the epididymis, spermatozoa are non-motile and the long axis of their nuclei is perpendicular to that of the tail. The nucleus is V-shaped with a thick and a thin arm. At the junction of the two arms. At the junction of the two arms, where the arms join, the articular fossa receives the capitulum of the connecting piece which attaches the head of the spermatozoon to the tail. Spermatozoa from the central region of the epididymis show a re-orientation of the nuclei which now lie parallel to the long axis of the tail. Both paired and unpaired spermatozoa are found and show an increase in motility. In the caudal region of the epididymis all spermatozoa are paired. Pairing occurs as a result of the close apposition of adjacent cell membranes covering the acrosomes of the larger arms."}
{"id": "PMID:464752", "title": "Specific lamellar structures of agranular endoplasmic reticulum in the senile mouse adrenal cortex.", "content": "In the adrenal cortex of old mice, lamellar bodies of various shapes frequently appear in the zona reticularis cells. These structures consist of electron dense plates, about 20 nm in width, closely piled at regular distances of about 25 nm. Each plate displays two sheets of unit membranes enclosing a dense material and with several clear spaces between them. These paired membranes usually terminate in round saccules at both ends, but occasionally continue to the tubules of surrounding agranular endoplasmic reticulum. Saccules in several rows, in combination with a part of the lamellae, may be arranged cirularly in the cytoplasm. From these observations, it is proposed that the lamellar body is composed of a cup-shaped mass of flattened cisternae of the agranular endoplasmic reticulum and each of the cisternae is attenuated and closed except at their marginal sacculations. The concentric or horseshoe-shaped profile of the lamellar body observed in this study is accounted for by different section planes through the cup-shaped structure.", "contents": "Specific lamellar structures of agranular endoplasmic reticulum in the senile mouse adrenal cortex. In the adrenal cortex of old mice, lamellar bodies of various shapes frequently appear in the zona reticularis cells. These structures consist of electron dense plates, about 20 nm in width, closely piled at regular distances of about 25 nm. Each plate displays two sheets of unit membranes enclosing a dense material and with several clear spaces between them. These paired membranes usually terminate in round saccules at both ends, but occasionally continue to the tubules of surrounding agranular endoplasmic reticulum. Saccules in several rows, in combination with a part of the lamellae, may be arranged cirularly in the cytoplasm. From these observations, it is proposed that the lamellar body is composed of a cup-shaped mass of flattened cisternae of the agranular endoplasmic reticulum and each of the cisternae is attenuated and closed except at their marginal sacculations. The concentric or horseshoe-shaped profile of the lamellar body observed in this study is accounted for by different section planes through the cup-shaped structure."}
{"id": "PMID:464753", "title": "A bioceramic endoprosthesis for the replacement of the proximal humerus.", "content": "In patients with malignant tumors in the region of the shoulder, radical resection can avoid amputation in most instances. To improve the function of the arm, endoprosthetic replacement of the defect is desirable. A three-component endoprosthesis made of a bioceramic material (aluminium oxide) was designed, implanted without bone cement. Fast anchorage to bone is achieved by using a conical sleeve, fixed upon the previously conically reamed humerus shaft. A stable primary fit is always feasible. Subsequent bone in growth into grooves inside the conical sleeve provides a permanent anchorage of the endoprosthesis. The authors experiences are based on implantations of 38 endoprostheses. The original diseases were primary malignant bone tumors in 19 patients, one case of \"solitary\" plasmocytoma and metastases into the proximal humerus in 16 patients. In two women, resection was made because of posttraumatic subcapital humeral pseudarthrosis. The follow-up study includes only those 27 cases operated on at least one year ago. 12 of the 14 patients with primary tumors have been surviving for 12-55 months (range 27.4) without signs of metastases or recurrent disease. Seven patients with metastases died of their original diseases after 7.7 months on the average. Owing to extensive resection of the shoulder musculature the mobility in the shoulder joint is considerably reduced. All the patients have good movement of the elbow joint and free function of the hand.", "contents": "A bioceramic endoprosthesis for the replacement of the proximal humerus. In patients with malignant tumors in the region of the shoulder, radical resection can avoid amputation in most instances. To improve the function of the arm, endoprosthetic replacement of the defect is desirable. A three-component endoprosthesis made of a bioceramic material (aluminium oxide) was designed, implanted without bone cement. Fast anchorage to bone is achieved by using a conical sleeve, fixed upon the previously conically reamed humerus shaft. A stable primary fit is always feasible. Subsequent bone in growth into grooves inside the conical sleeve provides a permanent anchorage of the endoprosthesis. The authors experiences are based on implantations of 38 endoprostheses. The original diseases were primary malignant bone tumors in 19 patients, one case of \"solitary\" plasmocytoma and metastases into the proximal humerus in 16 patients. In two women, resection was made because of posttraumatic subcapital humeral pseudarthrosis. The follow-up study includes only those 27 cases operated on at least one year ago. 12 of the 14 patients with primary tumors have been surviving for 12-55 months (range 27.4) without signs of metastases or recurrent disease. Seven patients with metastases died of their original diseases after 7.7 months on the average. Owing to extensive resection of the shoulder musculature the mobility in the shoulder joint is considerably reduced. All the patients have good movement of the elbow joint and free function of the hand."}
{"id": "PMID:464754", "title": "Improvements in total hip prosthesis implantation technique. A cement-proof seal for the lower medullary cavity and a dihedral self-stabilizing trochanteric osteotomy.", "content": "The anchorage of the femoral component of a total hip prosthesis can be improved by the following two refinements to the surgical technique: 1. The femoral medullary cavity is plugged with a bone core taken from the excised femoral head or with a polyethylene bung. The plug prevents the bone cement from being displaced distally into the lower part of the medullary cavity. It contains a small hole through which blood can escape, thus preventing the latter from accumulating in the upper part of the medullary cavity and impairing the bond between the cement and the bone wall of the cavity. 2. The greater trochanter is no longer sliced off with a single cut in one plane but is now removed by dihedral osteotomy, i.e., by making cuts in two planes which intersect each other so as to form a ridge. On compressing this osteotomy by tension band fixation all movement between the fragments is eliminated, including that in the parasagittal plane which results from flexion and extension of the hip. In this manner loosening and pseudarthrosis of the osteotomy are prevented.", "contents": "Improvements in total hip prosthesis implantation technique. A cement-proof seal for the lower medullary cavity and a dihedral self-stabilizing trochanteric osteotomy. The anchorage of the femoral component of a total hip prosthesis can be improved by the following two refinements to the surgical technique: 1. The femoral medullary cavity is plugged with a bone core taken from the excised femoral head or with a polyethylene bung. The plug prevents the bone cement from being displaced distally into the lower part of the medullary cavity. It contains a small hole through which blood can escape, thus preventing the latter from accumulating in the upper part of the medullary cavity and impairing the bond between the cement and the bone wall of the cavity. 2. The greater trochanter is no longer sliced off with a single cut in one plane but is now removed by dihedral osteotomy, i.e., by making cuts in two planes which intersect each other so as to form a ridge. On compressing this osteotomy by tension band fixation all movement between the fragments is eliminated, including that in the parasagittal plane which results from flexion and extension of the hip. In this manner loosening and pseudarthrosis of the osteotomy are prevented."}
{"id": "PMID:464755", "title": "Special instruments and prosthetic cups for the removal and replacement of a total hip prosthesis.", "content": "In the course of replacing more than 500 total hip prostheses over a period of five years the authors have developed a new set of instruments for the removal of the defective prosthesis and cement and for improved anchorage of the new prosthetic cup. The set comprises: 1. A set of special chisels and an axially guided drill for removal of the old cement from the femur. These instruments greatly simplify the latter procedure. 2. A new type of prosthetic cup with a weight-bearing collar. This cup is used for cases in which the amount or structure of the acetabular bone is such that it will not support a conventional cup. 3. A new type of drill with a flexible drive and easily exchangeable drill bits for cutting anchorage holes in otherwise inaccessible parts of the acetabular bone.", "contents": "Special instruments and prosthetic cups for the removal and replacement of a total hip prosthesis. In the course of replacing more than 500 total hip prostheses over a period of five years the authors have developed a new set of instruments for the removal of the defective prosthesis and cement and for improved anchorage of the new prosthetic cup. The set comprises: 1. A set of special chisels and an axially guided drill for removal of the old cement from the femur. These instruments greatly simplify the latter procedure. 2. A new type of prosthetic cup with a weight-bearing collar. This cup is used for cases in which the amount or structure of the acetabular bone is such that it will not support a conventional cup. 3. A new type of drill with a flexible drive and easily exchangeable drill bits for cutting anchorage holes in otherwise inaccessible parts of the acetabular bone."}
{"id": "PMID:464756", "title": "Behavior of the extensor pollicis longus tendon in experiment.", "content": "Several experimental rupture tests of the extensor pollicis longus tendon proved that a rupture of a tendon caused by experiments is located in a region in which the rupture is mainly found clinically as well. By using a newly developed Silicon impression system it can be proved that in the course of a tendon there is always a certain place which has the smallest cross section. This is more or less identical with the location of the rupture. Thus, ruptures of the extensor pollicis longus tendon can be experimentally simulated.", "contents": "Behavior of the extensor pollicis longus tendon in experiment. Several experimental rupture tests of the extensor pollicis longus tendon proved that a rupture of a tendon caused by experiments is located in a region in which the rupture is mainly found clinically as well. By using a newly developed Silicon impression system it can be proved that in the course of a tendon there is always a certain place which has the smallest cross section. This is more or less identical with the location of the rupture. Thus, ruptures of the extensor pollicis longus tendon can be experimentally simulated."}
{"id": "PMID:464757", "title": "[The significance of tomography in the x-ray diagnosis of coxarthrosis (author's transl)].", "content": "Authors have made besides routine roentgenograms tomograms too in 50 patients with coxarthrosis of various severeness. For an assessment of the diagnostic value of tomography the routine roentgenograms and the tomograms were analysed comparatively. The investigations have shown that the tomography may give in certain cases great help in the judgement of progression and in the correct indication of operative methods in hip surgery.", "contents": "[The significance of tomography in the x-ray diagnosis of coxarthrosis (author's transl)]. Authors have made besides routine roentgenograms tomograms too in 50 patients with coxarthrosis of various severeness. For an assessment of the diagnostic value of tomography the routine roentgenograms and the tomograms were analysed comparatively. The investigations have shown that the tomography may give in certain cases great help in the judgement of progression and in the correct indication of operative methods in hip surgery."}
{"id": "PMID:464758", "title": "Total subperiosteal resection treatment of solitary bone cysts of the humerus.", "content": "The solitary bone cyst is most frequently located in the upper arm and the average age of the affected patients is between 7 and 9 years, thus perceptibly lower than in cases where solitary bone cyst occurs elsewhere, where the average age is 15. The tendency towards recurrence before 10 years of age is twice as great as the tendency after that age. Investigation of the results obtained from the treatment of 26 patients suffering from solitary bone cyst of the humerus showed a recurrence rate of 55% after curettage and filling-in of the defect with cancellous bone grafts, whereas after total subperiosteal resection and bridging the defect with an autologous tibia graft the corresponding recurrence frequency was 7%. The average duration of the plaster cast fixing period after resection treatment was 18 days longer than after curettage, but the low rate of recurrence in the first-mentioned case makes up for this disadvantage. It is absolutely essential to retain the periosteum in cases of cyst resections. The defect is bridged over by an autologous tibia graft, but fibula grafts are also suitable for bridging the defect. Osteosyntheses are not necessary with latent cysts. In the case of active cysts screws, wire loops, Kirschner wires, and thin K\u00fcntscher nails can be used as temporary stabilisation means. Plate osteosyntheses constitute an exception. Complete removal of the cyst by resection is the most certain prophylactic method against recurrence, and hence the most reliable form of treatment of the solitary bone cyst of the humerus.", "contents": "Total subperiosteal resection treatment of solitary bone cysts of the humerus. The solitary bone cyst is most frequently located in the upper arm and the average age of the affected patients is between 7 and 9 years, thus perceptibly lower than in cases where solitary bone cyst occurs elsewhere, where the average age is 15. The tendency towards recurrence before 10 years of age is twice as great as the tendency after that age. Investigation of the results obtained from the treatment of 26 patients suffering from solitary bone cyst of the humerus showed a recurrence rate of 55% after curettage and filling-in of the defect with cancellous bone grafts, whereas after total subperiosteal resection and bridging the defect with an autologous tibia graft the corresponding recurrence frequency was 7%. The average duration of the plaster cast fixing period after resection treatment was 18 days longer than after curettage, but the low rate of recurrence in the first-mentioned case makes up for this disadvantage. It is absolutely essential to retain the periosteum in cases of cyst resections. The defect is bridged over by an autologous tibia graft, but fibula grafts are also suitable for bridging the defect. Osteosyntheses are not necessary with latent cysts. In the case of active cysts screws, wire loops, Kirschner wires, and thin K\u00fcntscher nails can be used as temporary stabilisation means. Plate osteosyntheses constitute an exception. Complete removal of the cyst by resection is the most certain prophylactic method against recurrence, and hence the most reliable form of treatment of the solitary bone cyst of the humerus."}
{"id": "PMID:464759", "title": "An alternate method for posterior fixation in anterior and inferior atlanto-axial dislocation.", "content": "A modification of common methods is described for fixation of anterior and inferior atlanto-axial dislocation (AAD and IAD). In AAD a wire is passed around the atlas arch and knotted around the spinous process of the axis in a way that a figure 8 arrangement is achieved as seen in the lateral view. In IAD a wire is passed through holes in the occiput and knotted around the spinous process of the axis. A rectangular bone graft is inserted between the occiput and the spinous process of the axis, thus preventing further inferior dislocation. In both methods methyl methacrylate and bone grafts are as a rule added for further long-time stabilization. The methods have been used in 15 cases of AAD and in three cases of IAD. On radiologic examination with attention focused on the immediate postoperative fixation the follow-up time has been three months. The long-time results of fixation have been recorded with a follow-up time of six years. The fixation proved to be satisfactory in 14 patients with AAD and in the three patients with IAD. The clinical results were good in the 14 patients with AAD and in the three with IAD.", "contents": "An alternate method for posterior fixation in anterior and inferior atlanto-axial dislocation. A modification of common methods is described for fixation of anterior and inferior atlanto-axial dislocation (AAD and IAD). In AAD a wire is passed around the atlas arch and knotted around the spinous process of the axis in a way that a figure 8 arrangement is achieved as seen in the lateral view. In IAD a wire is passed through holes in the occiput and knotted around the spinous process of the axis. A rectangular bone graft is inserted between the occiput and the spinous process of the axis, thus preventing further inferior dislocation. In both methods methyl methacrylate and bone grafts are as a rule added for further long-time stabilization. The methods have been used in 15 cases of AAD and in three cases of IAD. On radiologic examination with attention focused on the immediate postoperative fixation the follow-up time has been three months. The long-time results of fixation have been recorded with a follow-up time of six years. The fixation proved to be satisfactory in 14 patients with AAD and in the three patients with IAD. The clinical results were good in the 14 patients with AAD and in the three with IAD."}
{"id": "PMID:464760", "title": "[The creeping post-operative hip joint empyema (author's transl)].", "content": "The case histories of 10 patients with chronic empyema of the hip joint following nailing of the femoral neck, respectively following intertrochanteric osteotomy are being described. With only a few exceptions their post-operative course showed no signs of infection. After an interval of between 5 weeks and 2 years their condition deteriorated rapidly. By the time the patients returned to hospital, usually, the joint was already destroyed or septic luxation had occurred. Because of their poor general condition only resection of the femoral head was possible. As an alternative to continuous suction drainage, the implantation of PMMA chains seems to have advantages.", "contents": "[The creeping post-operative hip joint empyema (author's transl)]. The case histories of 10 patients with chronic empyema of the hip joint following nailing of the femoral neck, respectively following intertrochanteric osteotomy are being described. With only a few exceptions their post-operative course showed no signs of infection. After an interval of between 5 weeks and 2 years their condition deteriorated rapidly. By the time the patients returned to hospital, usually, the joint was already destroyed or septic luxation had occurred. Because of their poor general condition only resection of the femoral head was possible. As an alternative to continuous suction drainage, the implantation of PMMA chains seems to have advantages."}
{"id": "PMID:464761", "title": "Myositis ossificans following total knee replacement. A report on two cases.", "content": "Two cases of myositis ossificans involving the quadriceps femoris muscle are described following total knee replacement arthroplasty. This condition appears to be a very rare complication of this operation. One case was helped by ultrasound therapy.", "contents": "Myositis ossificans following total knee replacement. A report on two cases. Two cases of myositis ossificans involving the quadriceps femoris muscle are described following total knee replacement arthroplasty. This condition appears to be a very rare complication of this operation. One case was helped by ultrasound therapy."}
{"id": "PMID:464762", "title": "The effects of dynamic loading on intracalcaneal wood implants and on the tissues surrounding them.", "content": "The dorsal parts of the calcanei of rabbits were resected and replaced with similarly-shaped implants made of ash wood. In each case the Achilles tendon was reinserted into the projecting part of the implant so as to subject the latter to the dynamic loads generated by use of the limb. The implants were removed from some of the animals after 5 weeks and from the others after 14 weeks. They were evaluated by preparing undecalcified microtome sections and ground sections which were examined by light microscopy and microradiography. Some of the animals underwent vital staining so as to produce polychromatic sequential labelling of the calcaneal specimens. In the area of insertion of the Achilles tendon soft tissue grew into the larger pores of the wood and, in some cases, differentiated into cartilage. Every implant remained stably anchored in the calcaneus. In every animal the intracalcaneal part of the implant became surrounded by new bone which was in direct contact with the surface of the implant and which also formed in the pores of the wood, even all rabbits loaded the operated foot. The formation of new bone in and around the implants demonstrates the basic feasibility of using ash wood as an isoelastic implant material in bone.", "contents": "The effects of dynamic loading on intracalcaneal wood implants and on the tissues surrounding them. The dorsal parts of the calcanei of rabbits were resected and replaced with similarly-shaped implants made of ash wood. In each case the Achilles tendon was reinserted into the projecting part of the implant so as to subject the latter to the dynamic loads generated by use of the limb. The implants were removed from some of the animals after 5 weeks and from the others after 14 weeks. They were evaluated by preparing undecalcified microtome sections and ground sections which were examined by light microscopy and microradiography. Some of the animals underwent vital staining so as to produce polychromatic sequential labelling of the calcaneal specimens. In the area of insertion of the Achilles tendon soft tissue grew into the larger pores of the wood and, in some cases, differentiated into cartilage. Every implant remained stably anchored in the calcaneus. In every animal the intracalcaneal part of the implant became surrounded by new bone which was in direct contact with the surface of the implant and which also formed in the pores of the wood, even all rabbits loaded the operated foot. The formation of new bone in and around the implants demonstrates the basic feasibility of using ash wood as an isoelastic implant material in bone."}
{"id": "PMID:464763", "title": "Differential diagnosis of ruptures of the lateral ligaments of the ankle joint.", "content": "Ruptures of the lateral ligaments of the ankle joint occur very frequently in young persons and this subject is therefore one which deserves more attention. Once a fracture or avulsion of a ligamentary insertion has been excluded the nature and extent of the ligamentary lesion should be precisely assessed. The only objective criterion of rupture of a ligament is demonstrable instability of the joint. Since the anterior talo-fibular ligament is always found to be torn immediately following supination-inversion injury to the ligaments the talar drawer sign will be demonstratable both clinically and radiologically in such cases. This test causes almost no pain and can be carried out without anesthesia. The radiological visualization of ligamentary instability with the aid of a simple jig which stresses the ankle joint provides diagnostic information which is definitely superior to that obtained from an a-p roentegenogram of the stressed joint. This conclusion was reached by statistical analysis of 384 roentgenograms.", "contents": "Differential diagnosis of ruptures of the lateral ligaments of the ankle joint. Ruptures of the lateral ligaments of the ankle joint occur very frequently in young persons and this subject is therefore one which deserves more attention. Once a fracture or avulsion of a ligamentary insertion has been excluded the nature and extent of the ligamentary lesion should be precisely assessed. The only objective criterion of rupture of a ligament is demonstrable instability of the joint. Since the anterior talo-fibular ligament is always found to be torn immediately following supination-inversion injury to the ligaments the talar drawer sign will be demonstratable both clinically and radiologically in such cases. This test causes almost no pain and can be carried out without anesthesia. The radiological visualization of ligamentary instability with the aid of a simple jig which stresses the ankle joint provides diagnostic information which is definitely superior to that obtained from an a-p roentegenogram of the stressed joint. This conclusion was reached by statistical analysis of 384 roentgenograms."}
{"id": "PMID:464764", "title": "[Crystallographic investigation of distraction callus (author's transl)].", "content": "Physical examination using electron diffraction and thermogravimetry was performed on the distraction callus of six metacarpal bones of sheep. Brushite was observed in this callus beside apatite, and the number of crystals found was less than in physiological nature bone. From the crystallographic point of view the distraction callus was found similar to the callus known in fracture healing.", "contents": "[Crystallographic investigation of distraction callus (author's transl)]. Physical examination using electron diffraction and thermogravimetry was performed on the distraction callus of six metacarpal bones of sheep. Brushite was observed in this callus beside apatite, and the number of crystals found was less than in physiological nature bone. From the crystallographic point of view the distraction callus was found similar to the callus known in fracture healing."}
{"id": "PMID:464765", "title": "Syndromes of compression of the median nerve in the proximal forearm (pronator teres syndrome; anterior interosseous nerve syndrome).", "content": "Entrapment of the median nerve in the proximal forearm is seen in two forms: the pronator teres syndrome, and the anterior interosseous nerve (or Kiloh-Nevin) syndrome. Both syndromes are rare, and they comprised approximately 1% of the compression syndromes of the upper limb which were treated operatively by the authors. The symptoms, signs, etiologies, and intraoperative findings are discussed. It is pointed out that certain of the clinical features may resemble those of irritation of the median nerve by a supracondylar process or Struthers' ligament. Although both proximal median entrapment syndromes have a favorable prognosis when treated non-operatively, the authors recommend operative treatment in cases in which there is no perceptible improvement following 8 weeks of non-operative treatment, since this is likely to speed and enhance recovery. Nine cases of the pronator teres syndrome (8 treated successfully by operation, 1 failure) and 2 cases of the anterior interosseous nerve syndrome (both fully recovered) are added to the cases reported previously in the literature.", "contents": "Syndromes of compression of the median nerve in the proximal forearm (pronator teres syndrome; anterior interosseous nerve syndrome). Entrapment of the median nerve in the proximal forearm is seen in two forms: the pronator teres syndrome, and the anterior interosseous nerve (or Kiloh-Nevin) syndrome. Both syndromes are rare, and they comprised approximately 1% of the compression syndromes of the upper limb which were treated operatively by the authors. The symptoms, signs, etiologies, and intraoperative findings are discussed. It is pointed out that certain of the clinical features may resemble those of irritation of the median nerve by a supracondylar process or Struthers' ligament. Although both proximal median entrapment syndromes have a favorable prognosis when treated non-operatively, the authors recommend operative treatment in cases in which there is no perceptible improvement following 8 weeks of non-operative treatment, since this is likely to speed and enhance recovery. Nine cases of the pronator teres syndrome (8 treated successfully by operation, 1 failure) and 2 cases of the anterior interosseous nerve syndrome (both fully recovered) are added to the cases reported previously in the literature."}
{"id": "PMID:464766", "title": "Femoral neck fractures following surface replacement.", "content": "A femoral neck fracture following surface replacement of the hip has been studied and five potential causes of failure were noted. These consisted of; removal of a hip nail during surgery, use of a too small femoral cup, initial reaming in varus, notching of the femoral neck, and failure to completely seat the cup.", "contents": "Femoral neck fractures following surface replacement. A femoral neck fracture following surface replacement of the hip has been studied and five potential causes of failure were noted. These consisted of; removal of a hip nail during surgery, use of a too small femoral cup, initial reaming in varus, notching of the femoral neck, and failure to completely seat the cup."}
{"id": "PMID:464767", "title": "Functional recovery from cancer surgery: estimation of expectations.", "content": "Level of expected functional recovery from colostomy, laryngectomy and mastectomy was established by the Delphi technique. One hundred fifteen cancer rehabilitation experts responded to 2 rounds of questionnaires and provided their judgments regarding degrees and timing of recovery in 14 functional, physical and psychological areas. Feedback of first round judgments was provided in the second round and 85% agreement was set as the standard for consensus. The mastectomy patient is expected to have the quickest recovery with former level of functioning reached in most areas by 3 months postdischarge. The colostomy patient is expected to be functioning normally in most areas by 6 months postdischarge. The laryngectomy patient is expected to be functioning normally in most areas by 12 months postdischarge. Some functions were judged to have the same rate of recovery for all 3 patient types; for example, the experts agreed that for the 55-year-old patients described in the case abstracts, sexual activities would not reach the preoperative frequency until 12 months postdischarge.", "contents": "Functional recovery from cancer surgery: estimation of expectations. Level of expected functional recovery from colostomy, laryngectomy and mastectomy was established by the Delphi technique. One hundred fifteen cancer rehabilitation experts responded to 2 rounds of questionnaires and provided their judgments regarding degrees and timing of recovery in 14 functional, physical and psychological areas. Feedback of first round judgments was provided in the second round and 85% agreement was set as the standard for consensus. The mastectomy patient is expected to have the quickest recovery with former level of functioning reached in most areas by 3 months postdischarge. The colostomy patient is expected to be functioning normally in most areas by 6 months postdischarge. The laryngectomy patient is expected to be functioning normally in most areas by 12 months postdischarge. Some functions were judged to have the same rate of recovery for all 3 patient types; for example, the experts agreed that for the 55-year-old patients described in the case abstracts, sexual activities would not reach the preoperative frequency until 12 months postdischarge."}
{"id": "PMID:464768", "title": "The pedynograph: a clinical tool for force measurement and gait analysis in lower extremity amputees.", "content": "The pedynograph is a simple, inexpensive piece of equipment which can be used in the clinical setting to monitor amputee gait. Strain gauges applied to the pylon of a modular prosthesis and incorporated in an appropriate electrical circuit provide measurements of axial load which are displayed on an oscilloscope during ambulation. Preliminary experience with the described equipment has shown that it is of value in monitoring and regulating weight bearing in patients with healing or maturing stumps or impairment of stump sensation. Gait and prosthetic problems can be analyzed, their correction documented and a permanent record of amputee performance provided.", "contents": "The pedynograph: a clinical tool for force measurement and gait analysis in lower extremity amputees. The pedynograph is a simple, inexpensive piece of equipment which can be used in the clinical setting to monitor amputee gait. Strain gauges applied to the pylon of a modular prosthesis and incorporated in an appropriate electrical circuit provide measurements of axial load which are displayed on an oscilloscope during ambulation. Preliminary experience with the described equipment has shown that it is of value in monitoring and regulating weight bearing in patients with healing or maturing stumps or impairment of stump sensation. Gait and prosthetic problems can be analyzed, their correction documented and a permanent record of amputee performance provided."}
{"id": "PMID:464769", "title": "Orthotic devices for serratus anterior palsy: some biomechanical considerations.", "content": "The pathomechanics of serratus anterior palsy are reviewed to provide a physiological basis for designing an effective brace. The problems involved in the practical application of the brace and the effectiveness of the brace in controlling the biomechanical dysfunctions are discussed. Two modifications of the basic brace and their effects on the patients' shoulder functions are presented. The main benefit of scapular bracing is control of scapular winging, while loss of active scapular rotation and medial scapular displacement cannot be effectively corrected.", "contents": "Orthotic devices for serratus anterior palsy: some biomechanical considerations. The pathomechanics of serratus anterior palsy are reviewed to provide a physiological basis for designing an effective brace. The problems involved in the practical application of the brace and the effectiveness of the brace in controlling the biomechanical dysfunctions are discussed. Two modifications of the basic brace and their effects on the patients' shoulder functions are presented. The main benefit of scapular bracing is control of scapular winging, while loss of active scapular rotation and medial scapular displacement cannot be effectively corrected."}
{"id": "PMID:464770", "title": "Pressure distribution and skin temperature effect of the ROHO wheelchair balloon cushion.", "content": "This study evaluated the effectiveness of the ROHO Balloon Wheelchair Cushion in distributing pressure on the skin over the ischial tuberosities in healthy subjects. Surface skin temperatures over the ischial tuberosities and thighs of the subjects were also measured. Pressure recordings were obtained by placing a pressure sensor between the wheelchair cushion and the skin overlying the ischial tuberosities. For comparison, pressures were recorded while the same subjects sat on a 4-inch foam cushion supported on a plywood board. Skin surface temperatures on the 2 cushions were recorded on separate occasions under the ischial tuberosity and the posterior thigh during a 30-minute test period. The results indicate no important difference in resting pressures when the subjects sat on either the 4-inch foam cushion or the balloon cushion. Neither was there an important difference in pressure when the cushion was inflated to greater than the manufacturer's recommendations or when it was covered with a commercially designed cover. The average increase in skin temperature over the tuberosity was 1.9C and over the thigh, 2.6C.", "contents": "Pressure distribution and skin temperature effect of the ROHO wheelchair balloon cushion. This study evaluated the effectiveness of the ROHO Balloon Wheelchair Cushion in distributing pressure on the skin over the ischial tuberosities in healthy subjects. Surface skin temperatures over the ischial tuberosities and thighs of the subjects were also measured. Pressure recordings were obtained by placing a pressure sensor between the wheelchair cushion and the skin overlying the ischial tuberosities. For comparison, pressures were recorded while the same subjects sat on a 4-inch foam cushion supported on a plywood board. Skin surface temperatures on the 2 cushions were recorded on separate occasions under the ischial tuberosity and the posterior thigh during a 30-minute test period. The results indicate no important difference in resting pressures when the subjects sat on either the 4-inch foam cushion or the balloon cushion. Neither was there an important difference in pressure when the cushion was inflated to greater than the manufacturer's recommendations or when it was covered with a commercially designed cover. The average increase in skin temperature over the tuberosity was 1.9C and over the thigh, 2.6C."}
{"id": "PMID:464771", "title": "Involuntary single motor unit discharges in spastic muscles during EMG biofeedback training.", "content": "The apparent involuntary single motor unit discharges observed in many extremity muscles of spastic patients necessitate monitoring raw electromyography during training sessions. The clinician should note that electronic components of some biofeedback instruments may amplify and distort these units so that the feedback, whether by meter or by variable pitch tone, may erroneously be perceived by the patient as a high level of muscle tension.", "contents": "Involuntary single motor unit discharges in spastic muscles during EMG biofeedback training. The apparent involuntary single motor unit discharges observed in many extremity muscles of spastic patients necessitate monitoring raw electromyography during training sessions. The clinician should note that electronic components of some biofeedback instruments may amplify and distort these units so that the feedback, whether by meter or by variable pitch tone, may erroneously be perceived by the patient as a high level of muscle tension."}
{"id": "PMID:464772", "title": "Below-knee orthosis: a wrap-around design for ankle-foot control.", "content": "Control of foot and ankle positions with orthotic appliances is generally more difficult to accomplish in children with spastic than flaccid paralytic disorders. Our new design for a custom molded plastic ankle-foot orthosis has proven superior to other designs in most applications. The appliance is vacuum molded from very thin polypropylene plastic and innerfaced with a foam material (Aliplast). The limb is enclosed completely in plastic with an anterior opening secured by several Velcro straps. Although the material is soft and pliable, the circumferential support provides remarkably rigid control of the limb. The orthosis has been successfully applied to children with polio, meningomyelocele and all forms of spastic and athetoid cerebral palsy. In addition to superior limb control, another advantage appears to be reflex inhibition of abnormal motor tone in may spastic patients. Patients have preferred this orthosis to those previously worn probably due to greater comfort. The limitation of adjustability of dorsiflexion can be compensated by heel or sole shoe lifts. Excessive heat created by the \"wrap-around\" design can be reduced by a thin cotton sock extended above the orthosis.", "contents": "Below-knee orthosis: a wrap-around design for ankle-foot control. Control of foot and ankle positions with orthotic appliances is generally more difficult to accomplish in children with spastic than flaccid paralytic disorders. Our new design for a custom molded plastic ankle-foot orthosis has proven superior to other designs in most applications. The appliance is vacuum molded from very thin polypropylene plastic and innerfaced with a foam material (Aliplast). The limb is enclosed completely in plastic with an anterior opening secured by several Velcro straps. Although the material is soft and pliable, the circumferential support provides remarkably rigid control of the limb. The orthosis has been successfully applied to children with polio, meningomyelocele and all forms of spastic and athetoid cerebral palsy. In addition to superior limb control, another advantage appears to be reflex inhibition of abnormal motor tone in may spastic patients. Patients have preferred this orthosis to those previously worn probably due to greater comfort. The limitation of adjustability of dorsiflexion can be compensated by heel or sole shoe lifts. Excessive heat created by the \"wrap-around\" design can be reduced by a thin cotton sock extended above the orthosis."}
{"id": "PMID:464777", "title": "Psychosocial factors associated with pressure sores.", "content": "To determine if psychosocial factors, not purely mechanical aspects, play an important part in the incidence of pressure sores, 141 subjects were tested on 3 psychosocial measures. Results indicate that differences exist among groups in pressure sore history and psychosocial measures. Contrary to the expectations of a mechanical skin problem model, quadriplegics as a group (rather than paraplegics) had a history of fewer pressure sores. Two variables, the subject's responsibility in skin care (RESPON) and his satisfaction with the activiities of life (SATIS), were significantly associated with the incidence of decubitus ulcers. The combination of RESPON, SATIS, and the total positive score from the Tennessee Self-Concept Scale in multiple linear regression accounted for (1) 26% of the pressure sore history in the total sample, (2) 40% in the paraplegic group, (3) 69% in the paraplegic with help group, and (4) 41% in the paraplegic without help group. The histories in the quadriplegic groups and subgroups did not show reliable associations with the 3 variables. SATIS contributed most in accounting for the incidence of pressure sores. The level of injury and these psychosocial factors are thus related to the incidence of pressure sores.", "contents": "Psychosocial factors associated with pressure sores. To determine if psychosocial factors, not purely mechanical aspects, play an important part in the incidence of pressure sores, 141 subjects were tested on 3 psychosocial measures. Results indicate that differences exist among groups in pressure sore history and psychosocial measures. Contrary to the expectations of a mechanical skin problem model, quadriplegics as a group (rather than paraplegics) had a history of fewer pressure sores. Two variables, the subject's responsibility in skin care (RESPON) and his satisfaction with the activiities of life (SATIS), were significantly associated with the incidence of decubitus ulcers. The combination of RESPON, SATIS, and the total positive score from the Tennessee Self-Concept Scale in multiple linear regression accounted for (1) 26% of the pressure sore history in the total sample, (2) 40% in the paraplegic group, (3) 69% in the paraplegic with help group, and (4) 41% in the paraplegic without help group. The histories in the quadriplegic groups and subgroups did not show reliable associations with the 3 variables. SATIS contributed most in accounting for the incidence of pressure sores. The level of injury and these psychosocial factors are thus related to the incidence of pressure sores."}
{"id": "PMID:464778", "title": "Conceptual style and life satisfaction following spinal cord injury.", "content": "The major purpose of this study was to explore the relationship between conceptual style and life satisfaction following a physical loss. It was predicted that conceptual abstractness is positively associated with life satisfaction. Fifty-four men between the ages of 18 and 41 who were paraplegic or quadriplegic, because of a spinal cord injury that occurred at least 18 months prior to the interview, were asked to complete questionnaires designed to measure their conceptual styles and their satisfaction with various aspects of their lives. The data suggest that: the respondents were generally satisfied with life; dissatisfaction was frequently greatest in family and other intimate relationships; and physical trauma may trigger changes in conceptual style in the direction of greater complexity and abstractness. The predicted positive association between conceptual abstractness and life satisfaction was supported in several instances by the data.", "contents": "Conceptual style and life satisfaction following spinal cord injury. The major purpose of this study was to explore the relationship between conceptual style and life satisfaction following a physical loss. It was predicted that conceptual abstractness is positively associated with life satisfaction. Fifty-four men between the ages of 18 and 41 who were paraplegic or quadriplegic, because of a spinal cord injury that occurred at least 18 months prior to the interview, were asked to complete questionnaires designed to measure their conceptual styles and their satisfaction with various aspects of their lives. The data suggest that: the respondents were generally satisfied with life; dissatisfaction was frequently greatest in family and other intimate relationships; and physical trauma may trigger changes in conceptual style in the direction of greater complexity and abstractness. The predicted positive association between conceptual abstractness and life satisfaction was supported in several instances by the data."}
{"id": "PMID:464779", "title": "Sphenopalatine (nasal) ganglion: remote effects including \"psychosomatic\" symptoms, rage reaction, pain, and spasm.", "content": "Many articles implicate the nasal ganglion in the production of remote symptoms and discuss treatment. Symptoms are primarily spastic, involving both visceral and voluntary muscles including muscle spasm in the neck, shoulder, and low back; asthma, hypertension, intestinal spasm; diarrhea, angina pectoris, uterine spasm; intractable hiccup, and many others. All these symptoms appear to have 2 common denominators. They are mediated by the autonomic nervous system and at least in some instances can be \"psychosomatic.\" The sphenopalatine ganglion (SPG) is a major autonomic ganglion located superficially in the pterygopalatine fossa, with major afferent distribution to the entire nasopharynx and important connections with the trigeminal nerve, facial nerve, internal carotid artery plexus of the sympathetic nervous system and, as shown in the rat, direct connection with the anterior pituitary gland. This paper presents arguments supporting the following hypotheses: 1. The SPG probably has a crucial role in lower animals in declenching the reflex responses known collectively as the rage reaction. 2. The SPG is a major point of entry to the autonomic system exposed to pathologic influences and readily accessible for therapeutic influences and readily accessible for therapeutic intervention. 3. A wide variety of symptoms are produced or maintained by alteration in autonomic system tonus and some of these may be affected by intervention on the SPG. 4. The possible relationship of some symptoms and \"psychosomatic\" conditions to the autonomic nervous system and the rage reaction must be considered.20", "contents": "Sphenopalatine (nasal) ganglion: remote effects including \"psychosomatic\" symptoms, rage reaction, pain, and spasm. Many articles implicate the nasal ganglion in the production of remote symptoms and discuss treatment. Symptoms are primarily spastic, involving both visceral and voluntary muscles including muscle spasm in the neck, shoulder, and low back; asthma, hypertension, intestinal spasm; diarrhea, angina pectoris, uterine spasm; intractable hiccup, and many others. All these symptoms appear to have 2 common denominators. They are mediated by the autonomic nervous system and at least in some instances can be \"psychosomatic.\" The sphenopalatine ganglion (SPG) is a major autonomic ganglion located superficially in the pterygopalatine fossa, with major afferent distribution to the entire nasopharynx and important connections with the trigeminal nerve, facial nerve, internal carotid artery plexus of the sympathetic nervous system and, as shown in the rat, direct connection with the anterior pituitary gland. This paper presents arguments supporting the following hypotheses: 1. The SPG probably has a crucial role in lower animals in declenching the reflex responses known collectively as the rage reaction. 2. The SPG is a major point of entry to the autonomic system exposed to pathologic influences and readily accessible for therapeutic influences and readily accessible for therapeutic intervention. 3. A wide variety of symptoms are produced or maintained by alteration in autonomic system tonus and some of these may be affected by intervention on the SPG. 4. The possible relationship of some symptoms and \"psychosomatic\" conditions to the autonomic nervous system and the rage reaction must be considered.20"}
{"id": "PMID:464780", "title": "Electromyographic insertional activity in normal limb muscles.", "content": "A mechanical electrode inserter was used to allow reproducible electromyographic (emg) electrode insertions. The electrical activity provoked by the mechanical insertion was recorded and quantitatively analyzed in the anterior tibialis, the extensor hallucis longus, and the extensor digitorum brevis of unimpaired human subjects. No statistically significant differences were found among these muscles in the total duration of sharp spike activity or in the duration of sharp spike activity following cessation of needle electrode movement. There was a statistically significant reduction in the total duration of mechanically-provoked electrical activity in the extensor digitorum brevis when compared to the anterior tibialis using the technique employed in this study. There was a statistically significant increase in time following the onset of needle electrode movement until the initiation of sharp spike activity in the extensor hallucis longus as compared to the anterior tibialis. Abnormalities are present in the extensor digitorum brevis muscle of clinically unimpaired subjects. Endplate spikes can be recorded as positive-shaped potentials in the endplate zones of muscle. Nonreproducible positive-shaped potentials of unknown etiology are seen to occur singly or in pairs following insertional activity in normal muscle. When examining muscle for the early evidence of provokable positive sharp wave and fibrillation potentials, care must be taken not to mistake normal characteristics of insertional activity for pathological abnormalities.", "contents": "Electromyographic insertional activity in normal limb muscles. A mechanical electrode inserter was used to allow reproducible electromyographic (emg) electrode insertions. The electrical activity provoked by the mechanical insertion was recorded and quantitatively analyzed in the anterior tibialis, the extensor hallucis longus, and the extensor digitorum brevis of unimpaired human subjects. No statistically significant differences were found among these muscles in the total duration of sharp spike activity or in the duration of sharp spike activity following cessation of needle electrode movement. There was a statistically significant reduction in the total duration of mechanically-provoked electrical activity in the extensor digitorum brevis when compared to the anterior tibialis using the technique employed in this study. There was a statistically significant increase in time following the onset of needle electrode movement until the initiation of sharp spike activity in the extensor hallucis longus as compared to the anterior tibialis. Abnormalities are present in the extensor digitorum brevis muscle of clinically unimpaired subjects. Endplate spikes can be recorded as positive-shaped potentials in the endplate zones of muscle. Nonreproducible positive-shaped potentials of unknown etiology are seen to occur singly or in pairs following insertional activity in normal muscle. When examining muscle for the early evidence of provokable positive sharp wave and fibrillation potentials, care must be taken not to mistake normal characteristics of insertional activity for pathological abnormalities."}
{"id": "PMID:464781", "title": "EMG needle electrodes: electrical impedance.", "content": "The electrical impedance of samples of stainless-steel monopolar needle electrodes and concentric electrodes from 3 manufacturers were measured in 154mM saline solution using sine-wave excitation at 4 frequencies from 10Hz to 10kHz. Current densities of less than 2.5x10(-5) amp/cm2 were used. Measurements on both needle and fine-wire electrodes were also made in live rats. Linear dimensions of the exposed tips were measured from which the areas of exposure were calculated. Surface areas of the monopolar needles varied between 0.06 and 0.15mm2. Presoaking of the electrodes for 20 minutes in saline solution containing a small concentration of a wetting agent produced a sixfold to twentyfold reduction in impedance. The average impedance magnitudes of the monopolar electrodes ranged from 1.4 megohms (Momega) at 10Hz to 6.6 kolohms (komega at 10kHz. The phase angle of the impedance due to the capacitive component of the needle-electrolyte impedance ranged between -45 and -71 degrees. The reduction in impedance resulting from presoaking and the concomitant reduction in electronic noise would be an advantage in observing lowamplitude potentials.", "contents": "EMG needle electrodes: electrical impedance. The electrical impedance of samples of stainless-steel monopolar needle electrodes and concentric electrodes from 3 manufacturers were measured in 154mM saline solution using sine-wave excitation at 4 frequencies from 10Hz to 10kHz. Current densities of less than 2.5x10(-5) amp/cm2 were used. Measurements on both needle and fine-wire electrodes were also made in live rats. Linear dimensions of the exposed tips were measured from which the areas of exposure were calculated. Surface areas of the monopolar needles varied between 0.06 and 0.15mm2. Presoaking of the electrodes for 20 minutes in saline solution containing a small concentration of a wetting agent produced a sixfold to twentyfold reduction in impedance. The average impedance magnitudes of the monopolar electrodes ranged from 1.4 megohms (Momega) at 10Hz to 6.6 kolohms (komega at 10kHz. The phase angle of the impedance due to the capacitive component of the needle-electrolyte impedance ranged between -45 and -71 degrees. The reduction in impedance resulting from presoaking and the concomitant reduction in electronic noise would be an advantage in observing lowamplitude potentials."}
{"id": "PMID:464782", "title": "Vocational development and adjustment of adolescents with cystic fibrosis.", "content": "Adolescents with cystic fibrosis were compared with normal adolescents of the same age and educational grade on several measures of vocational development and adjustment. The cystic fibrosis group in ages 12--16 and in grades 7, 8, and 9 scored lower than their normal counterparts in measures of vocational and educational plans, and were less realistic than normals in considering their limitations and financial constraints. In contrast, the cystic fibrosis group at nearly all age and grade levels scored significantly higher on strength of commitment to vocational choice, work values, and awareness of occupational information. Compared to 4 additional handicapped groups already studied, adolescents with cystic fibrosis scored favorably. They not only plan to work but also hold strong work values compared to normals. Rehabilitation programs should emphasize preparation for a career and the development of educational and vocational plans while the adolescent is still in high school.", "contents": "Vocational development and adjustment of adolescents with cystic fibrosis. Adolescents with cystic fibrosis were compared with normal adolescents of the same age and educational grade on several measures of vocational development and adjustment. The cystic fibrosis group in ages 12--16 and in grades 7, 8, and 9 scored lower than their normal counterparts in measures of vocational and educational plans, and were less realistic than normals in considering their limitations and financial constraints. In contrast, the cystic fibrosis group at nearly all age and grade levels scored significantly higher on strength of commitment to vocational choice, work values, and awareness of occupational information. Compared to 4 additional handicapped groups already studied, adolescents with cystic fibrosis scored favorably. They not only plan to work but also hold strong work values compared to normals. Rehabilitation programs should emphasize preparation for a career and the development of educational and vocational plans while the adolescent is still in high school."}
{"id": "PMID:464783", "title": "Arthritic hand response to pressure gradient gloves.", "content": "Five women with osteoarthritis and 10 with rheumatoid arthritis and finger joint involvement wore a pressure gradient glove, a control glove, or no glove in a randomly assigned sequence. All were outpatients. Each patient wore the assigned glove nightly for 1 week, after which a battery of subjective and objective data, including number of tender joints, ring size, grip strength, palmar temperature, and hand function were obtained. In all, 105 separate examinaion sessions were completed over 7 visits, with each hand serving as its own control in the experimental design. The palmar skin temperature was elevated in both patient groups when either a pressure gradient or control glove was worn. In patients with rheumatoid arthritis, the only significant difference was that the pressure gradient glove reduced ring size when compared to the control glove or no glove. No differences were detected in the patients with osteoarthritis.", "contents": "Arthritic hand response to pressure gradient gloves. Five women with osteoarthritis and 10 with rheumatoid arthritis and finger joint involvement wore a pressure gradient glove, a control glove, or no glove in a randomly assigned sequence. All were outpatients. Each patient wore the assigned glove nightly for 1 week, after which a battery of subjective and objective data, including number of tender joints, ring size, grip strength, palmar temperature, and hand function were obtained. In all, 105 separate examinaion sessions were completed over 7 visits, with each hand serving as its own control in the experimental design. The palmar skin temperature was elevated in both patient groups when either a pressure gradient or control glove was worn. In patients with rheumatoid arthritis, the only significant difference was that the pressure gradient glove reduced ring size when compared to the control glove or no glove. No differences were detected in the patients with osteoarthritis."}
{"id": "PMID:464784", "title": "Neonatal spinal cord injury.", "content": "In this review of the prognosis of patients having neonatal spinal cord injury, findings in 3 patients and 11 case histories from the literature are evaluated as to obstetric complications, clinical and pathologic examinations, and follow-up data from 2 to 12 years, or until death of the child. Eight of the 14 patients died: 4 at less than 3 months of age, 3 between 3 months and 1 year of age, and 1 at 3 1/2 years of age. Six children survived for more than 2 years for follow-up examination. Survival is dependent on the level of the lesion, with cervical lesions being almost incompatible with life. The quality of survival for those with levels of C8-T1 and below depends on the child's multiple medical complications.", "contents": "Neonatal spinal cord injury. In this review of the prognosis of patients having neonatal spinal cord injury, findings in 3 patients and 11 case histories from the literature are evaluated as to obstetric complications, clinical and pathologic examinations, and follow-up data from 2 to 12 years, or until death of the child. Eight of the 14 patients died: 4 at less than 3 months of age, 3 between 3 months and 1 year of age, and 1 at 3 1/2 years of age. Six children survived for more than 2 years for follow-up examination. Survival is dependent on the level of the lesion, with cervical lesions being almost incompatible with life. The quality of survival for those with levels of C8-T1 and below depends on the child's multiple medical complications."}
{"id": "PMID:464785", "title": "Dysphagia associated with cricopharyngeal dysfunction.", "content": "In protracted forms of dysphagia associated with neuromuscular dysfunction, myotomy of the upper esophageal sphincter has been suggested. The literature, however, is unclear about the indications and outcome of this procedure. In this article, 3 cases are presented of dysphagia associated with the failure of relaxation of the cricopharyngeus during swallowing. Two patients had brain stem infarctions and the 3rd had an inflammatory disease of the brain stem. In all patients, barium swallow revealed aspiration of contrast material into the trachea with failure of relaxation of the cricopharyngeal sphincter. Indirect laryngoscopy demonstrated partial paralysis of one or both vocal cords. In one, an emg of the laryngeal muscles showed normal results. A complete evaluation by the speech pathologist failed to reveal abnormality of the oral musculature in all patients. All patients required gastrostomies for their nutritional needs. Therapeutic modification and control of rate, quantity and consistency of food along with counseling for prevention of aspiration. Oral feeding, without aspiration, was achieved in the 3 patients within the treatment period, allowing removal of the gastrostomy. Cricopharyngeal myotomy, therefore, was not necessary in these patients, despite the long duration of the swallowing problem.", "contents": "Dysphagia associated with cricopharyngeal dysfunction. In protracted forms of dysphagia associated with neuromuscular dysfunction, myotomy of the upper esophageal sphincter has been suggested. The literature, however, is unclear about the indications and outcome of this procedure. In this article, 3 cases are presented of dysphagia associated with the failure of relaxation of the cricopharyngeus during swallowing. Two patients had brain stem infarctions and the 3rd had an inflammatory disease of the brain stem. In all patients, barium swallow revealed aspiration of contrast material into the trachea with failure of relaxation of the cricopharyngeal sphincter. Indirect laryngoscopy demonstrated partial paralysis of one or both vocal cords. In one, an emg of the laryngeal muscles showed normal results. A complete evaluation by the speech pathologist failed to reveal abnormality of the oral musculature in all patients. All patients required gastrostomies for their nutritional needs. Therapeutic modification and control of rate, quantity and consistency of food along with counseling for prevention of aspiration. Oral feeding, without aspiration, was achieved in the 3 patients within the treatment period, allowing removal of the gastrostomy. Cricopharyngeal myotomy, therefore, was not necessary in these patients, despite the long duration of the swallowing problem."}
{"id": "PMID:464787", "title": "[Effects of oxygen inhalation therapy on blood and phonomechanography in patients with heart disease, lung disease and cardiorespiratory insufficiency].", "content": "Arterial PaO2 and some phonomechanographic indices were studied during and after a period of 40-60-100% oxygen breathing in fourteen patients (six with pulmonary insufficiency, four with cardiac disease and four with cardiorespiratory insufficiency). Arterial PaO2 was measured at the beginning, every five minutes during and many times after the ventilation. Myocardial performance was also measured by sistolic time: isovolumetric contraction time, left ventricular expulsive time, preejection pressure, deformation time. The authors discuss the modifications of these indices and the clinical involvement of O2 breathing.", "contents": "[Effects of oxygen inhalation therapy on blood and phonomechanography in patients with heart disease, lung disease and cardiorespiratory insufficiency]. Arterial PaO2 and some phonomechanographic indices were studied during and after a period of 40-60-100% oxygen breathing in fourteen patients (six with pulmonary insufficiency, four with cardiac disease and four with cardiorespiratory insufficiency). Arterial PaO2 was measured at the beginning, every five minutes during and many times after the ventilation. Myocardial performance was also measured by sistolic time: isovolumetric contraction time, left ventricular expulsive time, preejection pressure, deformation time. The authors discuss the modifications of these indices and the clinical involvement of O2 breathing."}
{"id": "PMID:464786", "title": "[Mexiletine, a drug with anti-arrhythmic action. Experimental research on the cardiovascular system].", "content": "It was shown experimentally that mexilithene possesses anti-arrhythmic activity with slight depression of sino-carotid baroreceptorial and glomo-carotid and gangliar chemoreceptorial activity and catecholamine uptake. Hypotensive and bradycardizing effects were only noted when high per kg doses were used. The drug did not display vascular alpha- and beta-adrenolytic, anti-muscarinic and anti-histaminic activity.", "contents": "[Mexiletine, a drug with anti-arrhythmic action. Experimental research on the cardiovascular system]. It was shown experimentally that mexilithene possesses anti-arrhythmic activity with slight depression of sino-carotid baroreceptorial and glomo-carotid and gangliar chemoreceptorial activity and catecholamine uptake. Hypotensive and bradycardizing effects were only noted when high per kg doses were used. The drug did not display vascular alpha- and beta-adrenolytic, anti-muscarinic and anti-histaminic activity."}
{"id": "PMID:464788", "title": "[Changes of plasma renin activity during perfusion with dextran 40. Functional study; statistical evaluations and cl-nical considerations].", "content": "Changes in RAP, AP (max and min), Ht and R.R. induced by daily perfusion with 250 and 500 ml Dextran 40 in a 10% isotonic solution were examined in two groups of patients with peripheral obstructive arteriopathy. Systemic AP showed no appreciable changes, whereas RAP and Ht fell in direct proportion of the dose given. This effect tended to disappear after 96 hr. Administration of this plasma volume extender 4 days per week is recommended.", "contents": "[Changes of plasma renin activity during perfusion with dextran 40. Functional study; statistical evaluations and cl-nical considerations]. Changes in RAP, AP (max and min), Ht and R.R. induced by daily perfusion with 250 and 500 ml Dextran 40 in a 10% isotonic solution were examined in two groups of patients with peripheral obstructive arteriopathy. Systemic AP showed no appreciable changes, whereas RAP and Ht fell in direct proportion of the dose given. This effect tended to disappear after 96 hr. Administration of this plasma volume extender 4 days per week is recommended."}
{"id": "PMID:464789", "title": "[Relations between immunological and enzymatic parameters in viral hepatitis].", "content": "The results of a study showing correlations between the behaviour of complement serum level, the presence of antigen Au, variations in serum immunoglobulin and the behaviour of transaminases in the cases of hepatitis examined are reported. It was found that the reduction in complement level during the initial stage of the disease coincides directly with antigenaemia positivity, increased IgM production, large increases in transaminase activity and inversion of the SGOT/SGPT ratio.", "contents": "[Relations between immunological and enzymatic parameters in viral hepatitis]. The results of a study showing correlations between the behaviour of complement serum level, the presence of antigen Au, variations in serum immunoglobulin and the behaviour of transaminases in the cases of hepatitis examined are reported. It was found that the reduction in complement level during the initial stage of the disease coincides directly with antigenaemia positivity, increased IgM production, large increases in transaminase activity and inversion of the SGOT/SGPT ratio."}
{"id": "PMID:464792", "title": "[Ectopic pancreas in the duodenum].", "content": "Aberrant pancreas to the duodenal site is a by no means simple problem of diagnosis as it is only rarely that X-ray, gastroscopy or biopsy suggest it. The case of aberrant pancreas in the duodenum, simulating a peptic lesion, is reported. Following an anatomo-pathological study, the typical aspects of the case are stressed.", "contents": "[Ectopic pancreas in the duodenum]. Aberrant pancreas to the duodenal site is a by no means simple problem of diagnosis as it is only rarely that X-ray, gastroscopy or biopsy suggest it. The case of aberrant pancreas in the duodenum, simulating a peptic lesion, is reported. Following an anatomo-pathological study, the typical aspects of the case are stressed."}
{"id": "PMID:464793", "title": "[Zieve's syndrome. Case report].", "content": "The Zieve's syndrome consists of the transient association of cholestatic jaundice, hemolytic anemia, hyperlipemia, in a chronic alcoholic. The Authors, after a short introduction regarding physiopathological problems, describe and subsequently examine on the clinical ground a recent personal observation, which had been mentioned for the rarene-s of this disease.", "contents": "[Zieve's syndrome. Case report]. The Zieve's syndrome consists of the transient association of cholestatic jaundice, hemolytic anemia, hyperlipemia, in a chronic alcoholic. The Authors, after a short introduction regarding physiopathological problems, describe and subsequently examine on the clinical ground a recent personal observation, which had been mentioned for the rarene-s of this disease."}
{"id": "PMID:464794", "title": "[Behavior of plasma androgens in cases of adrenal pathology].", "content": "Following chromatographic separation, the RI method has been used to examine the behaviour of plasmatic androgens in a group of normal subjects, in two patients with Addison's disease and two patients with hypercorticoadrenalism, due respectively to corticoadrenal adenoma and bilateral adenomatous hyperplasia. A significant reduction in adrenal androgens was observed in patients suffering from Addison's disease. The most interesting information, however, was the paradoxical reduction in DHEA and delta 4-A in patients with corticoadrenal hyperfunction.", "contents": "[Behavior of plasma androgens in cases of adrenal pathology]. Following chromatographic separation, the RI method has been used to examine the behaviour of plasmatic androgens in a group of normal subjects, in two patients with Addison's disease and two patients with hypercorticoadrenalism, due respectively to corticoadrenal adenoma and bilateral adenomatous hyperplasia. A significant reduction in adrenal androgens was observed in patients suffering from Addison's disease. The most interesting information, however, was the paradoxical reduction in DHEA and delta 4-A in patients with corticoadrenal hyperfunction."}
{"id": "PMID:464795", "title": "[A case of primary hyperparathyroidism. Clinical contribution].", "content": "The authors discusses some clinical, laboratory and instrumental problems of a reported case of primary hyperparathyroidism caused by adenoma from the point of view of medical literature. Nethertheless the lack of the specificity of the clinical manifestations and the slowly progressive evolution of this disease, primary hyperparatiroidism must be suspected in the presence of his most common multifarious complications. The finding of hypercalcemia in these conditions and from screening procedures requests parathyroid functional tests and exclusion of other causes of which chiefly pseudohyperparathyroidism may be difficult to be differentiated. The early diagnosis and correction of the parathyroid lesion avoid further damage and regression of many preoperatory complications.", "contents": "[A case of primary hyperparathyroidism. Clinical contribution]. The authors discusses some clinical, laboratory and instrumental problems of a reported case of primary hyperparathyroidism caused by adenoma from the point of view of medical literature. Nethertheless the lack of the specificity of the clinical manifestations and the slowly progressive evolution of this disease, primary hyperparatiroidism must be suspected in the presence of his most common multifarious complications. The finding of hypercalcemia in these conditions and from screening procedures requests parathyroid functional tests and exclusion of other causes of which chiefly pseudohyperparathyroidism may be difficult to be differentiated. The early diagnosis and correction of the parathyroid lesion avoid further damage and regression of many preoperatory complications."}
{"id": "PMID:464796", "title": "[Methods of treatment of tumors of the soft parts and bones of the limbs and extremities. Analysis of case records].", "content": "218 patients examined for localized cancer of the extremities (50% benign, 50% malignant) are reported. The incidence is considered by sex and age. Demolition surgery was carried out in 35 patients and 27 locoregional treatment with antiblastics and radioactive isotopes was employed. Conservative surgery was used in 158. Operative mortality was less than 1% (2 cases out of 218) and did not reach 6% if amputations and disarticulations alone are considered. There were no deaths in cases submitted to radiochemotherapy and inguinocrural hymphadenectomy. The series includes a 15-years survival for giant-cell tumour of the femur treated with radioactive microsphores, an 11-years survival for a popliteal melanoma submitted to radiocolloid infiltration, a 9-year survival for femoral osteosarcoma for which hip disarticulation was carried out and a 4-year survival for a recurrent rhabdomyosarcoma of the hip operated by hemipelvectomy.", "contents": "[Methods of treatment of tumors of the soft parts and bones of the limbs and extremities. Analysis of case records]. 218 patients examined for localized cancer of the extremities (50% benign, 50% malignant) are reported. The incidence is considered by sex and age. Demolition surgery was carried out in 35 patients and 27 locoregional treatment with antiblastics and radioactive isotopes was employed. Conservative surgery was used in 158. Operative mortality was less than 1% (2 cases out of 218) and did not reach 6% if amputations and disarticulations alone are considered. There were no deaths in cases submitted to radiochemotherapy and inguinocrural hymphadenectomy. The series includes a 15-years survival for giant-cell tumour of the femur treated with radioactive microsphores, an 11-years survival for a popliteal melanoma submitted to radiocolloid infiltration, a 9-year survival for femoral osteosarcoma for which hip disarticulation was carried out and a 4-year survival for a recurrent rhabdomyosarcoma of the hip operated by hemipelvectomy."}
{"id": "PMID:464803", "title": "Further definition of antibiotic use and abuse in the surgical setting.", "content": "Surveys of the use of antimicrobial agents were conducted at three university-affiliated teaching hospitals to ascertain the current use of antibiotics associated with surgery and to compare this use to the presently accepted standards. The survey included 300 consecutive surgical patients representing three surgical specialities. Evaluation was based on accepted therapeutic criteria with respect to the presence of an infection, appropriate in vitro antibiotic sensitivity testing, and recognized forms of prophylaxis. The results of our survey disclosed that although nonuse was usually appropriate, more often than not use of antibiotics was inappropriate. Errors, in order of frequency, included (1) misjudgments in attempted prophylaxis of operative wound infection, such as failure to use preoperative administration or use in clean operations without implanted foreign bodies, such as prosthesis, and (2) attempted treatment of undefined and undiagnosed fever.", "contents": "Further definition of antibiotic use and abuse in the surgical setting. Surveys of the use of antimicrobial agents were conducted at three university-affiliated teaching hospitals to ascertain the current use of antibiotics associated with surgery and to compare this use to the presently accepted standards. The survey included 300 consecutive surgical patients representing three surgical specialities. Evaluation was based on accepted therapeutic criteria with respect to the presence of an infection, appropriate in vitro antibiotic sensitivity testing, and recognized forms of prophylaxis. The results of our survey disclosed that although nonuse was usually appropriate, more often than not use of antibiotics was inappropriate. Errors, in order of frequency, included (1) misjudgments in attempted prophylaxis of operative wound infection, such as failure to use preoperative administration or use in clean operations without implanted foreign bodies, such as prosthesis, and (2) attempted treatment of undefined and undiagnosed fever."}
{"id": "PMID:464804", "title": "Choledocholithiasis associated with acute cholecystitis.", "content": "A retrospective review of the records of 1,507 patients with a diagnosis of cholecystitis was conducted for the five-year period, 1972 to 1977. Of this group of patients, a histopathologic diagnosis of acute cholecystitis was established in 154 patients (10.2%). Common duct calculi were detected in 17 of these 154 patients, an incidence of 11%. Preoperative evaluation by means of serum bilirubin and alkaline phosphatase levels and intravenous cholangiography was unsatisfactory for consistent demonstration of choledocholithiasis in the presence of acute cholecystitis. Intraoperative cholangiography was found to be the most reliable method for detection of common duct calculi and was successfully employed in 14 of 17 patients with choledocholithiasis. The remaining three patients had palpable stones.", "contents": "Choledocholithiasis associated with acute cholecystitis. A retrospective review of the records of 1,507 patients with a diagnosis of cholecystitis was conducted for the five-year period, 1972 to 1977. Of this group of patients, a histopathologic diagnosis of acute cholecystitis was established in 154 patients (10.2%). Common duct calculi were detected in 17 of these 154 patients, an incidence of 11%. Preoperative evaluation by means of serum bilirubin and alkaline phosphatase levels and intravenous cholangiography was unsatisfactory for consistent demonstration of choledocholithiasis in the presence of acute cholecystitis. Intraoperative cholangiography was found to be the most reliable method for detection of common duct calculi and was successfully employed in 14 of 17 patients with choledocholithiasis. The remaining three patients had palpable stones."}
{"id": "PMID:464805", "title": "Ultrasonography and tumor-associated antigens. The concept of combining noninvasive tests in the screening for pancreatic cancer.", "content": "A total of 134 patients suspected of having pancreatic cancer were given preoperative ultrasonic examinations. A total of 54 cases had a final diagnosis of pancreatic cancer histologically confirmed. The diagnostic sensitivity and specificity of the ultrasonographies were 76% and 91% or 87% and 74%, depending on whether doubtful results were called abnormal or normal. \"Doubtful\" results were defined as those that stated that pancreatic cancer was \"possible\" or \"could not be excluded.\" When these results were divided into abnormal and normal groups on the basis of the patients' preoperative serum carcinoembryonic antigen and pancreatic oncofetal antigen levels, the sensitivity and specificity of the combined tests may prove to be a more accurate means of screening patients for pancreatic cancer than ultrasonography alone.", "contents": "Ultrasonography and tumor-associated antigens. The concept of combining noninvasive tests in the screening for pancreatic cancer. A total of 134 patients suspected of having pancreatic cancer were given preoperative ultrasonic examinations. A total of 54 cases had a final diagnosis of pancreatic cancer histologically confirmed. The diagnostic sensitivity and specificity of the ultrasonographies were 76% and 91% or 87% and 74%, depending on whether doubtful results were called abnormal or normal. \"Doubtful\" results were defined as those that stated that pancreatic cancer was \"possible\" or \"could not be excluded.\" When these results were divided into abnormal and normal groups on the basis of the patients' preoperative serum carcinoembryonic antigen and pancreatic oncofetal antigen levels, the sensitivity and specificity of the combined tests may prove to be a more accurate means of screening patients for pancreatic cancer than ultrasonography alone."}
{"id": "PMID:464806", "title": "Physical characteristics of implanted polytetrafluoroethylene grafts: a preliminary report.", "content": "Despite the widespread clinical use of polytetrafluoroethylene (PTFE) grafts, few reports dealing with their physical properties have appeared in the literature. In the past 20 months, 17 such grafts have been implanted into 15 patients threatened with limb loss, with an 88% immediate graft patency. Postoperatively, all patients underwent periodic evaluations; arteriograms were obtained in four. The effect of knee flexion on graft function was also studied. All grafts were found capable of withstanding cuff pressures in excess of 300 mm Hg without occlusion. With 90 degrees flexion, the grafts kinked crossing the knee joint but a pressure gradient or decrease in pulsatile blood flow did not develop. This remarkable resistance to external compression should render these grafts especially valuable for extra-anatomic bypass.", "contents": "Physical characteristics of implanted polytetrafluoroethylene grafts: a preliminary report. Despite the widespread clinical use of polytetrafluoroethylene (PTFE) grafts, few reports dealing with their physical properties have appeared in the literature. In the past 20 months, 17 such grafts have been implanted into 15 patients threatened with limb loss, with an 88% immediate graft patency. Postoperatively, all patients underwent periodic evaluations; arteriograms were obtained in four. The effect of knee flexion on graft function was also studied. All grafts were found capable of withstanding cuff pressures in excess of 300 mm Hg without occlusion. With 90 degrees flexion, the grafts kinked crossing the knee joint but a pressure gradient or decrease in pulsatile blood flow did not develop. This remarkable resistance to external compression should render these grafts especially valuable for extra-anatomic bypass."}
{"id": "PMID:464797", "title": "[Long-term results of 2 continuous series of total hip prostheses of the McKee-Farrar metallo-metal type and the Charnley metallo-plastic type].", "content": "Two continuous series of McKee-Farrar type metal-metal and Charnley type metal-plastic prostheses have been examined. Results are analysed and complications examined ten years after the operation. The McKee-Farrar prostheses showed inferior results in all parameters and a markedly higher incidence of late complications compared to the Charnley prostheses.", "contents": "[Long-term results of 2 continuous series of total hip prostheses of the McKee-Farrar metallo-metal type and the Charnley metallo-plastic type]. Two continuous series of McKee-Farrar type metal-metal and Charnley type metal-plastic prostheses have been examined. Results are analysed and complications examined ten years after the operation. The McKee-Farrar prostheses showed inferior results in all parameters and a markedly higher incidence of late complications compared to the Charnley prostheses."}
{"id": "PMID:464799", "title": "[The role of digital storage in radiological studies based on fluoroscopic technics].", "content": "In recording radiological images obtained by fluoroscopic techniques on permanent support, digital storage, when compared with other methods, shows several interesting features, especially in order to reduce the X-ray dose and to allow for using economical hard copies. In the paper a system is described, for which it is possible to predict competitive cost in the near future; such system is based on a 512 X 512 X 6 bits digital memory, continuosly refreshed and on a software package oriented to handle radiological images. Examples of images referring to typical body segments (stomach, skull, chest) are reported.", "contents": "[The role of digital storage in radiological studies based on fluoroscopic technics]. In recording radiological images obtained by fluoroscopic techniques on permanent support, digital storage, when compared with other methods, shows several interesting features, especially in order to reduce the X-ray dose and to allow for using economical hard copies. In the paper a system is described, for which it is possible to predict competitive cost in the near future; such system is based on a 512 X 512 X 6 bits digital memory, continuosly refreshed and on a software package oriented to handle radiological images. Examples of images referring to typical body segments (stomach, skull, chest) are reported."}
{"id": "PMID:464807", "title": "Doxycycline in the prevention of hepatic dysfunction: an evaluation of its use following jejunoileal bypass in humans.", "content": "A prospective randomized study was carried out to evaluate doxycycline hyclate in the prevention of hepatic dysfunction following jejunoileal bypass for morbid obesity. Forty-five patients were entered into the study and were observed for a year or more. Patients received either 100 mg of doxycycline hyclate twice daily on postoperative days 5 through 10 and then 100 mg daily for six weeks, or no drug. At six weeks, 12 weeks, and 12 months after the operation, biochemical levels were measured. After one year all patients had a liver biopsy. Doxycycline, in the regimen, was not demonstrated to be of value in the prevention of hepatic dysfunction in these patients. A lack of correlation was demonstrated between the biochemical definition of hepatic dysfunction and liver histopathology.", "contents": "Doxycycline in the prevention of hepatic dysfunction: an evaluation of its use following jejunoileal bypass in humans. A prospective randomized study was carried out to evaluate doxycycline hyclate in the prevention of hepatic dysfunction following jejunoileal bypass for morbid obesity. Forty-five patients were entered into the study and were observed for a year or more. Patients received either 100 mg of doxycycline hyclate twice daily on postoperative days 5 through 10 and then 100 mg daily for six weeks, or no drug. At six weeks, 12 weeks, and 12 months after the operation, biochemical levels were measured. After one year all patients had a liver biopsy. Doxycycline, in the regimen, was not demonstrated to be of value in the prevention of hepatic dysfunction in these patients. A lack of correlation was demonstrated between the biochemical definition of hepatic dysfunction and liver histopathology."}
{"id": "PMID:464808", "title": "Long-term results of esophagomyotomy for achalasia of esophagus.", "content": "Modified Heller's esophagomyotomy for achalasia of the esophagus was done in 145 patients at Henry Ford Hospital, Detroit, from 1951 to 1977. Information on current symptoms was obtained for 121 patients from a detailed questionnaire that was sent to all patients, from personal interviews, or from data obtained from patients' clinical records. Average period of follow-up was 85 months. Actuarial analysis of postoperative symptoms showed an incidence of reflux of 24% after one year and 48% after ten years; incidence of relief of dysphagia was 89% of all patients after one year and 81% after ten years. Continual surveillance of patients after esophagomyotomy must be stressed. Dissatisfaction with the results of this procedure prompted us to recommend that an antireflux operation be performed at the time of the initial procedure.", "contents": "Long-term results of esophagomyotomy for achalasia of esophagus. Modified Heller's esophagomyotomy for achalasia of the esophagus was done in 145 patients at Henry Ford Hospital, Detroit, from 1951 to 1977. Information on current symptoms was obtained for 121 patients from a detailed questionnaire that was sent to all patients, from personal interviews, or from data obtained from patients' clinical records. Average period of follow-up was 85 months. Actuarial analysis of postoperative symptoms showed an incidence of reflux of 24% after one year and 48% after ten years; incidence of relief of dysphagia was 89% of all patients after one year and 81% after ten years. Continual surveillance of patients after esophagomyotomy must be stressed. Dissatisfaction with the results of this procedure prompted us to recommend that an antireflux operation be performed at the time of the initial procedure."}
{"id": "PMID:464809", "title": "Is there postdefecation bacteremia?", "content": "An investigation was done to determine the incidence of postdefecation bacteremia. A study of 82 healthy volunteers was done. Among 164 predefecation cultures, only one (0.6%) from an anaerobic flask was positive for microorganism, which was identified as Staphylococcus epidermidis. In only two of 328 (0.6%) postdefecation cultures did microorganisms grow, which were shown to be Propionibacterium acnes. The results of this study suggest that either bacteremia after defecation does not occur or is a rare event in healthy individuals.", "contents": "Is there postdefecation bacteremia? An investigation was done to determine the incidence of postdefecation bacteremia. A study of 82 healthy volunteers was done. Among 164 predefecation cultures, only one (0.6%) from an anaerobic flask was positive for microorganism, which was identified as Staphylococcus epidermidis. In only two of 328 (0.6%) postdefecation cultures did microorganisms grow, which were shown to be Propionibacterium acnes. The results of this study suggest that either bacteremia after defecation does not occur or is a rare event in healthy individuals."}
{"id": "PMID:464810", "title": "Early failure of expanded polytetrafluoroethylene in femoral vein replacement.", "content": "Grafts of expanded 30-mu fibril length polytetrafluoroethylene (PTFE) were inserted as segmental femoral vein replacements in nine dogs. The contralateral femoral vein served as a control, receiving a graft from each dog's right external jugular vein. Graft patency was monitored for 24 weeks postoperatively with serial venograms and venous pressures of the operated limbs. All expanded PTFE grafts and one autogenous graft thrombosed within 24 to 48 hours. Significant venous hypertension in the extremities receiving the PTFE grafts persisted for six months.", "contents": "Early failure of expanded polytetrafluoroethylene in femoral vein replacement. Grafts of expanded 30-mu fibril length polytetrafluoroethylene (PTFE) were inserted as segmental femoral vein replacements in nine dogs. The contralateral femoral vein served as a control, receiving a graft from each dog's right external jugular vein. Graft patency was monitored for 24 weeks postoperatively with serial venograms and venous pressures of the operated limbs. All expanded PTFE grafts and one autogenous graft thrombosed within 24 to 48 hours. Significant venous hypertension in the extremities receiving the PTFE grafts persisted for six months."}
{"id": "PMID:464811", "title": "Polypoid lesion of the stomach: resection through the fiberoptic endoscope.", "content": "Endoscopic removal of gastric polyps was done in six patients. Because most gastric polyps are benign and the malignant neoplasm is limited to the polyp without distant metastasis, local excision should be the treatment of choice.", "contents": "Polypoid lesion of the stomach: resection through the fiberoptic endoscope. Endoscopic removal of gastric polyps was done in six patients. Because most gastric polyps are benign and the malignant neoplasm is limited to the polyp without distant metastasis, local excision should be the treatment of choice."}
{"id": "PMID:464812", "title": "Renal cell carcinoma occurring with contralateral adrenal metastasis: a clinical and pathological trap.", "content": "Because renal cell carcinoma occasionally occurs in manifestations referable to a single metastatic lesion, there is a risk that a metastasis will be mistaken for the primary tumor. Histologic examination can compound the confusion, particularly when a clear cell carcinoma of the kidney metastasizes to an organ, such as lung, liver, adrenal, or skin, where clear cell tumors may occur as primary lesions. Although advanced renal cell carcinoma not infrequently involves the adrenal gland, the clinical and pathologic setting establishes the tumor in the adrenal as a metastasis. Two patients are described to illustrate what is to our knowledge the heretofore undescribed occurrence of renal cell carcinoma as a metastasis to the contralateral adrenal gland.", "contents": "Renal cell carcinoma occurring with contralateral adrenal metastasis: a clinical and pathological trap. Because renal cell carcinoma occasionally occurs in manifestations referable to a single metastatic lesion, there is a risk that a metastasis will be mistaken for the primary tumor. Histologic examination can compound the confusion, particularly when a clear cell carcinoma of the kidney metastasizes to an organ, such as lung, liver, adrenal, or skin, where clear cell tumors may occur as primary lesions. Although advanced renal cell carcinoma not infrequently involves the adrenal gland, the clinical and pathologic setting establishes the tumor in the adrenal as a metastasis. Two patients are described to illustrate what is to our knowledge the heretofore undescribed occurrence of renal cell carcinoma as a metastasis to the contralateral adrenal gland."}
{"id": "PMID:464813", "title": "Lower extremity arterial insufficiency after long-term methysergide maleate therapy. Its evaluation with Doppler ultrasonic velocity detector.", "content": "Iatrogenic ergotism is the primary source of ergot intoxication. The patient whose case is reviewed had migraine headaches and received methysergide maleate for 13 years. She had, in July 1977, severe claudication of the lower extremities. Measurements of the peripheral arterial circulation were made using the Doppler ultrasonic velocity detector. The extent of disease and subsequent reversal were documented using arteriographic examination. Initial measurements showed the patient was able to walk for one minute and 34 seconds on a treadmill (2 mph, 10% grade) before stopping because of claudication. Symptoms cleared after drug withdrawal and repeated testing produced no claudication. The calculated index (posterior tibial/arm pressure) increased from a mean of 0.22 to 0.74 during the eight-month period following discontinuance of methysergide therapy with no recurrence of migraine headaches. A review of the literature is also presented.", "contents": "Lower extremity arterial insufficiency after long-term methysergide maleate therapy. Its evaluation with Doppler ultrasonic velocity detector. Iatrogenic ergotism is the primary source of ergot intoxication. The patient whose case is reviewed had migraine headaches and received methysergide maleate for 13 years. She had, in July 1977, severe claudication of the lower extremities. Measurements of the peripheral arterial circulation were made using the Doppler ultrasonic velocity detector. The extent of disease and subsequent reversal were documented using arteriographic examination. Initial measurements showed the patient was able to walk for one minute and 34 seconds on a treadmill (2 mph, 10% grade) before stopping because of claudication. Symptoms cleared after drug withdrawal and repeated testing produced no claudication. The calculated index (posterior tibial/arm pressure) increased from a mean of 0.22 to 0.74 during the eight-month period following discontinuance of methysergide therapy with no recurrence of migraine headaches. A review of the literature is also presented."}
{"id": "PMID:464814", "title": "Perforated appendicitis in an incarcerated inguinal hernia.", "content": "Although perforated appendicitis in an incarcerated hernia is an uncommon condition, the clinical manifestations vary from relatively benign to much more serious, depending on whether the septic process is limited to the hernial canal and sac or if there is intraperitoneal contamination. The patient described in this report had minimal symptoms other than a slightly tender, irreducible inguinal hernia. He easily tolerated resection of the appendix and contiguous inguinal tissues, with a primary repair of the inguinal hernia.", "contents": "Perforated appendicitis in an incarcerated inguinal hernia. Although perforated appendicitis in an incarcerated hernia is an uncommon condition, the clinical manifestations vary from relatively benign to much more serious, depending on whether the septic process is limited to the hernial canal and sac or if there is intraperitoneal contamination. The patient described in this report had minimal symptoms other than a slightly tender, irreducible inguinal hernia. He easily tolerated resection of the appendix and contiguous inguinal tissues, with a primary repair of the inguinal hernia."}
{"id": "PMID:464815", "title": "Appendicitis and aortofemoral graft infection.", "content": "A 59-year-old man who underwent successful aortofemoral bypass had acute appendicitis at an indeterminate time in the postoperative period. Thirteen months later, a pulsatile groin mass developed. After a complicated course, it was found that infection from appendicitis had extended to the body of the graft. This unusual cause of graft infection reaffirms the importance of careful closure of the retroperitoneum over an aortic anastomosis and suggests a relationship of other intra-abdominal inflammatory processes to graft infection.", "contents": "Appendicitis and aortofemoral graft infection. A 59-year-old man who underwent successful aortofemoral bypass had acute appendicitis at an indeterminate time in the postoperative period. Thirteen months later, a pulsatile groin mass developed. After a complicated course, it was found that infection from appendicitis had extended to the body of the graft. This unusual cause of graft infection reaffirms the importance of careful closure of the retroperitoneum over an aortic anastomosis and suggests a relationship of other intra-abdominal inflammatory processes to graft infection."}
{"id": "PMID:464816", "title": "The surgical work day in the British Isles: some observations from a small sample studied in depth.", "content": "The daily activities of nine surgeons in the British Isles, representing a variety of practice settings, were observed. British surgeons had a mean total workweek of 45.8 hours, of which 34.5 hours were expended in professional activities. American community surgeons, as reported in the literature, had an analogous professional workweek of 28.7 hours, and American prepaid group surgeons, 50.7 hours. The American community surgeons performed many fewer operations, but the American prepaid group surgeons had operative loads similar to those of the British, although with a much greater time expenditure. Superior organization of British operating rooms in \"lists,\" the designation of certain operating days each week, a more rapid anesthesia induction and case turnover, all aided more rapid completion of a number of sequential surgical operations. Non-operative professional time expended per patient was less for the British group than any of the reported American groups.", "contents": "The surgical work day in the British Isles: some observations from a small sample studied in depth. The daily activities of nine surgeons in the British Isles, representing a variety of practice settings, were observed. British surgeons had a mean total workweek of 45.8 hours, of which 34.5 hours were expended in professional activities. American community surgeons, as reported in the literature, had an analogous professional workweek of 28.7 hours, and American prepaid group surgeons, 50.7 hours. The American community surgeons performed many fewer operations, but the American prepaid group surgeons had operative loads similar to those of the British, although with a much greater time expenditure. Superior organization of British operating rooms in \"lists,\" the designation of certain operating days each week, a more rapid anesthesia induction and case turnover, all aided more rapid completion of a number of sequential surgical operations. Non-operative professional time expended per patient was less for the British group than any of the reported American groups."}
{"id": "PMID:464819", "title": "[Effect of truly digestible lysine on live weight gain in rats].", "content": "It is to be proved in model experiments with rats that calculations with true digestible amino acids are more exact than calculations with the gross amount of amino acids and therefore are advantageous. 7 resp. 6 rations were tested whose gross contents of lysin, methionine and cystin were balanced. Their content of actually digestible lysin differed, however. The results of the two test series show distinctly that the prediction resp. assessment of the animals' performance in dependence on the amino acid content of the rations is most safely based on the actually digestible amino acids.", "contents": "[Effect of truly digestible lysine on live weight gain in rats]. It is to be proved in model experiments with rats that calculations with true digestible amino acids are more exact than calculations with the gross amount of amino acids and therefore are advantageous. 7 resp. 6 rations were tested whose gross contents of lysin, methionine and cystin were balanced. Their content of actually digestible lysin differed, however. The results of the two test series show distinctly that the prediction resp. assessment of the animals' performance in dependence on the amino acid content of the rations is most safely based on the actually digestible amino acids."}
{"id": "PMID:464820", "title": "[Protein digestability and amino acid absorption in various segments of the digestive tract of pigs. 1. Methods of animal experimentation, protein content and amino acid patterns of the digesta in the small intestine and in feces after feeding different feeds].", "content": "Re-entrance cannulae were applied by way of operations at the end of the small intestine of a larger number of growing pigs. After a post-operative phase these animals were fed with various rations (N-free diet, fattening feed for pigs, rations with dried skim milk resp. wheat gluten + lysine and wheat + wheat gluten + lysine). During the main experiment periods the ileum digesta (24 hour-periods of collecting) and the feces were quantitatively registered. The protein and amino acid content ascertained in the feed rations, the digesta and feces samples are reported, comparatively evaluated and discussed. The amount of amino acids (in mg per kg intake of dry matter) contained in the ileum digesta after N-free feeding were statistically calculated with regard to differences occuring between the animals and days. For most amino acids a significant decrease in the ileum digesta could be detected during the course of the N-free feeding period. Moreover, the amount of amino acids in the ileum digesta was compared with the metabolic fecal amino acids, from which conclusions could be drawn concerning the disappearance rate of the individual amino acids in large intestine.", "contents": "[Protein digestability and amino acid absorption in various segments of the digestive tract of pigs. 1. Methods of animal experimentation, protein content and amino acid patterns of the digesta in the small intestine and in feces after feeding different feeds]. Re-entrance cannulae were applied by way of operations at the end of the small intestine of a larger number of growing pigs. After a post-operative phase these animals were fed with various rations (N-free diet, fattening feed for pigs, rations with dried skim milk resp. wheat gluten + lysine and wheat + wheat gluten + lysine). During the main experiment periods the ileum digesta (24 hour-periods of collecting) and the feces were quantitatively registered. The protein and amino acid content ascertained in the feed rations, the digesta and feces samples are reported, comparatively evaluated and discussed. The amount of amino acids (in mg per kg intake of dry matter) contained in the ileum digesta after N-free feeding were statistically calculated with regard to differences occuring between the animals and days. For most amino acids a significant decrease in the ileum digesta could be detected during the course of the N-free feeding period. Moreover, the amount of amino acids in the ileum digesta was compared with the metabolic fecal amino acids, from which conclusions could be drawn concerning the disappearance rate of the individual amino acids in large intestine."}
{"id": "PMID:464821", "title": "Preliminary investigation on the nutritive value of krill meal in the feed of broiler chickens and laying hens.", "content": "Krill meal was used as a protein feed in rations for broiler chickens and laying hens and its nutritional effectiveness was studied in comparison with conventional feed-stuffs of animal origin. In experiments with 650 broiler chickens from 1 to 28 and from 29 to 56 days to fattening krill meal was added to the standard feedmixture in lieu of fish meal, and dried skim-milk, and at higher levels also in lieu of soyabean oilmeal. In the experiment with 22 layer hens kept in individual cages 3% of the fish meal were substituted by 3% of krill meal. The performance of chickens fed on diets with krill meal was lower in comparison with analogical groups fed on fish meal. Higher levels of krill meal, exceeding 15 and 11% in the first and second period of feeding respectively, reduced the weight gains and feed intake of chickens. The fatty acid C14, C18 and C18:1 content of internal body fat was changed in chickens fed on higher levels of krill meal. In the experiment with hens krill meal decreased the feed efficiency, the pigmentation of egg yolks, however, was more intensive and the vitamin A content of yolks was increased in comparison with the control group. The results show that krill meal can be used as a partial substitute of fish meal in the feed of broilers and hens.", "contents": "Preliminary investigation on the nutritive value of krill meal in the feed of broiler chickens and laying hens. Krill meal was used as a protein feed in rations for broiler chickens and laying hens and its nutritional effectiveness was studied in comparison with conventional feed-stuffs of animal origin. In experiments with 650 broiler chickens from 1 to 28 and from 29 to 56 days to fattening krill meal was added to the standard feedmixture in lieu of fish meal, and dried skim-milk, and at higher levels also in lieu of soyabean oilmeal. In the experiment with 22 layer hens kept in individual cages 3% of the fish meal were substituted by 3% of krill meal. The performance of chickens fed on diets with krill meal was lower in comparison with analogical groups fed on fish meal. Higher levels of krill meal, exceeding 15 and 11% in the first and second period of feeding respectively, reduced the weight gains and feed intake of chickens. The fatty acid C14, C18 and C18:1 content of internal body fat was changed in chickens fed on higher levels of krill meal. In the experiment with hens krill meal decreased the feed efficiency, the pigmentation of egg yolks, however, was more intensive and the vitamin A content of yolks was increased in comparison with the control group. The results show that krill meal can be used as a partial substitute of fish meal in the feed of broilers and hens."}
{"id": "PMID:464822", "title": "[Formation of the microcirculatory bed of the muscle coat of the wall of the sigmoid colon during human fetal development].", "content": "During intrauterine life microcirculatory bed of the muscular coat is forming and developing in accordance with its differentiation, growth and functional changes at various steps of its ontogenesis. This step-like differentiation in the parts composing the microcirculatory bed and the whole microcirculatory network of longitudinal and circulatory layers of the muscular coat is genetically connected with differentiation taking place in the wall of the sigmoid colon during the developmental period studied and, in the end, reflects morphological maturation of the sigmoid colon at different stages of its individual ontogenesis.", "contents": "[Formation of the microcirculatory bed of the muscle coat of the wall of the sigmoid colon during human fetal development]. During intrauterine life microcirculatory bed of the muscular coat is forming and developing in accordance with its differentiation, growth and functional changes at various steps of its ontogenesis. This step-like differentiation in the parts composing the microcirculatory bed and the whole microcirculatory network of longitudinal and circulatory layers of the muscular coat is genetically connected with differentiation taking place in the wall of the sigmoid colon during the developmental period studied and, in the end, reflects morphological maturation of the sigmoid colon at different stages of its individual ontogenesis."}
{"id": "PMID:464823", "title": "[State of the rat thyroid gland in coarctation of the abdominal aorta].", "content": "In order to study connections between blood vessels and follicular thyrocytes, the method of modulation (a purposeful change of state in one element with registration of states in other elements of the system) was used. In rats chronic increase of blood stream was produced in the thyroid gland; in 15 days it was 54% as high as in the control. The volume of the vascular bed increased by 28% and that of follicles by 26%. Volumetric ratio between the thyroid epithelium and colloid did not changed. Follicular thyrocytes grew high and the nuclear volumes of these cells increased. Thyrocytes greately varied in their height. The number of mast cells in the thyroid gland remained the same. Iodine absorption by the thyroid gland increased as it is dependent on the volume of the vascular bed of the organism (+0.82). The data obtained demonstrate a significant connection existing between the follicular thyrocytes and blood vessels.", "contents": "[State of the rat thyroid gland in coarctation of the abdominal aorta]. In order to study connections between blood vessels and follicular thyrocytes, the method of modulation (a purposeful change of state in one element with registration of states in other elements of the system) was used. In rats chronic increase of blood stream was produced in the thyroid gland; in 15 days it was 54% as high as in the control. The volume of the vascular bed increased by 28% and that of follicles by 26%. Volumetric ratio between the thyroid epithelium and colloid did not changed. Follicular thyrocytes grew high and the nuclear volumes of these cells increased. Thyrocytes greately varied in their height. The number of mast cells in the thyroid gland remained the same. Iodine absorption by the thyroid gland increased as it is dependent on the volume of the vascular bed of the organism (+0.82). The data obtained demonstrate a significant connection existing between the follicular thyrocytes and blood vessels."}
{"id": "PMID:464824", "title": "[Ultrastructural organization of the brush alveocytes of normal and hypertrophic rat lungs].", "content": "In non-inbred male rats, 140-200 g of body weight, the left lung was removed. In the right lung of the experimental and intact animals, brush alveolocytes were revealed electron microscopically and their structural organization was stated 1, 3, 7, 30 and 274 days after the operation. It was stated that during the process of compensatory hypertrophy in the lung, the occurrence rate of these cells increased considerably. In controls only 3 such cells were revealed, while in the experiment, the number of the brush alveolocytes was 20. In the experiment, most of the cells had signs of hyperfunction: increased amount of pinocytic vesicles in the apical part of the cell, dilated canaliculi in granular cytoplasmic network, formation of \"laminar\" structures from the membranes of the canaliculi of the granular cytoplasmic network. The brush alveolocytes were especially active during the period of acute alveolar hypertrophy (5-7 days after the operation) and correlated with the state of alveolar surfactant. The data obtained supported the hypothesis on chemoreceptor nature of the cells in question and demonstrated their participation in regulation of alveolar surface tension.", "contents": "[Ultrastructural organization of the brush alveocytes of normal and hypertrophic rat lungs]. In non-inbred male rats, 140-200 g of body weight, the left lung was removed. In the right lung of the experimental and intact animals, brush alveolocytes were revealed electron microscopically and their structural organization was stated 1, 3, 7, 30 and 274 days after the operation. It was stated that during the process of compensatory hypertrophy in the lung, the occurrence rate of these cells increased considerably. In controls only 3 such cells were revealed, while in the experiment, the number of the brush alveolocytes was 20. In the experiment, most of the cells had signs of hyperfunction: increased amount of pinocytic vesicles in the apical part of the cell, dilated canaliculi in granular cytoplasmic network, formation of \"laminar\" structures from the membranes of the canaliculi of the granular cytoplasmic network. The brush alveolocytes were especially active during the period of acute alveolar hypertrophy (5-7 days after the operation) and correlated with the state of alveolar surfactant. The data obtained supported the hypothesis on chemoreceptor nature of the cells in question and demonstrated their participation in regulation of alveolar surface tension."}
{"id": "PMID:464825", "title": "[Effect of orotic acid on reparative processes in the lungs following pneumonectomy].", "content": "The effect of orotic acid on pulmonary regeneration has been studied in mature rats after left-sided pneumonectomy. A complex morphometric analysis of the pulmonary parenchyma during the process of compensatory-hypertrophic rearrangement has been carried out; mitotic activity of cells in the interalveolar septae, peculiarities of DNA synthesis and those of enzymatic status in lymphocytes of the peripheral blood have been determined. As the investigations have demonstrated, the administration of orotic acid during the postoperative period contributes to a more complete regeneration of the lung volume, alveolar surface, results in formation of new alveoli, prevents the development of morphologic long-term decompensation following pneumonectomy. Activation of the alveolar cells proliferation and increased DNA synthesis are the main stimulating effects of orotic acid. Certain correlative connections are revealed between the course of pulmonary regenerative processes and the enzymic status of circulating lymphocytes.", "contents": "[Effect of orotic acid on reparative processes in the lungs following pneumonectomy]. The effect of orotic acid on pulmonary regeneration has been studied in mature rats after left-sided pneumonectomy. A complex morphometric analysis of the pulmonary parenchyma during the process of compensatory-hypertrophic rearrangement has been carried out; mitotic activity of cells in the interalveolar septae, peculiarities of DNA synthesis and those of enzymatic status in lymphocytes of the peripheral blood have been determined. As the investigations have demonstrated, the administration of orotic acid during the postoperative period contributes to a more complete regeneration of the lung volume, alveolar surface, results in formation of new alveoli, prevents the development of morphologic long-term decompensation following pneumonectomy. Activation of the alveolar cells proliferation and increased DNA synthesis are the main stimulating effects of orotic acid. Certain correlative connections are revealed between the course of pulmonary regenerative processes and the enzymic status of circulating lymphocytes."}
{"id": "PMID:464826", "title": "[Changes in rabbit thymus lymphoid tissue after administration of pyrogenal and hydrocortisone].", "content": "After a single administration of pyrogenal (0.2 and 5 mkg/kg) and hydrocortisone (100 mg/kg) histological changes in the rabbit thymus have been studied and compared with the level of 11-oxycorticosteroids (11-OCS) in blood of the animals under investigation. Hydrocortisone, while producing a considerable rise in the level of 11-OCS, increases the number of degenerating cells, inhibits mitotic activity and decreases the amount of lymphocytes per stipulated area unit in the cortical substance. Changes produced in the thymus after pyrogenal administration are similar to those produced by hydrocortisone administration but are pronounce much weaker, that is connected with concentration of corticosteroids circulating in blood.", "contents": "[Changes in rabbit thymus lymphoid tissue after administration of pyrogenal and hydrocortisone]. After a single administration of pyrogenal (0.2 and 5 mkg/kg) and hydrocortisone (100 mg/kg) histological changes in the rabbit thymus have been studied and compared with the level of 11-oxycorticosteroids (11-OCS) in blood of the animals under investigation. Hydrocortisone, while producing a considerable rise in the level of 11-OCS, increases the number of degenerating cells, inhibits mitotic activity and decreases the amount of lymphocytes per stipulated area unit in the cortical substance. Changes produced in the thymus after pyrogenal administration are similar to those produced by hydrocortisone administration but are pronounce much weaker, that is connected with concentration of corticosteroids circulating in blood."}
{"id": "PMID:464827", "title": "[Functional interrelationships of the sympathetic nervous system and the hypothalamo-hypophyseo-adrenal complex].", "content": "Adrenal and hypothalamic structure has been studied in mice at functional inhibition of the sympathetic region of the vegetative nerve system by means of antibodies against the nerve growth factor. Routine histological, histochemical, morphometric, electron microscopic and radioautographic methods have been applied. In the experiment the hypothalamic region is in the state of a continuous functional stress which is evident from enlargement of neurons and their nuclei in the macrocellular neurosecretory nuclei of the hypothalamus. The method of radioautography proves increasing activity in synthetic processes taking place in supraoptic, paraventricular and ventromedial nuclei of the hypothalamus and in the medial habenular nucleus of the epithalamus. In 30-day-old mice certain structural changes have been revealed in the adrenals demonstrating an increasing activity in their cortex: blood vessels are dilated, cholesterine, lipid and ascorbic acid granules are not evenly distributed, enzymatic activity in cytoplasm of adrenocorticocytes is increased. Ultramicroscopic destructive and regenerative alterations have no definite zonal specificity, nevertheless they are more pronounced in the fascicular zone. Radioautographic and morphometric investigations demonstrate an increasing functional activity in glomerular and fascicular adrenal zones. Taking into account morphologic demonstration of functional stress in hypophysial adenocytes in the same animals (Molostov O. K., 1974), it is possible to conclude that lesions in the sympathetic system center result in adaptive reaction of the hypothalamo-hypophysial system which, in its turn, produces reactions in the adrenals and in the vascular complex. This interconnection is accompanied by the reaction depending on breaking off direct sympathetic effects in the adrenals and vessels.", "contents": "[Functional interrelationships of the sympathetic nervous system and the hypothalamo-hypophyseo-adrenal complex]. Adrenal and hypothalamic structure has been studied in mice at functional inhibition of the sympathetic region of the vegetative nerve system by means of antibodies against the nerve growth factor. Routine histological, histochemical, morphometric, electron microscopic and radioautographic methods have been applied. In the experiment the hypothalamic region is in the state of a continuous functional stress which is evident from enlargement of neurons and their nuclei in the macrocellular neurosecretory nuclei of the hypothalamus. The method of radioautography proves increasing activity in synthetic processes taking place in supraoptic, paraventricular and ventromedial nuclei of the hypothalamus and in the medial habenular nucleus of the epithalamus. In 30-day-old mice certain structural changes have been revealed in the adrenals demonstrating an increasing activity in their cortex: blood vessels are dilated, cholesterine, lipid and ascorbic acid granules are not evenly distributed, enzymatic activity in cytoplasm of adrenocorticocytes is increased. Ultramicroscopic destructive and regenerative alterations have no definite zonal specificity, nevertheless they are more pronounced in the fascicular zone. Radioautographic and morphometric investigations demonstrate an increasing functional activity in glomerular and fascicular adrenal zones. Taking into account morphologic demonstration of functional stress in hypophysial adenocytes in the same animals (Molostov O. K., 1974), it is possible to conclude that lesions in the sympathetic system center result in adaptive reaction of the hypothalamo-hypophysial system which, in its turn, produces reactions in the adrenals and in the vascular complex. This interconnection is accompanied by the reaction depending on breaking off direct sympathetic effects in the adrenals and vessels."}
{"id": "PMID:464828", "title": "[Quantitative analysis of the structure of the varicose dilatations in the nerve fibers of the rabbit coronary artery (according to the results of three-dimensional reconstruction)].", "content": "Axoplasmic vesicles and microtubes in varicosities of axonal plexus in the external sheath of the rabbit coronary artery have been studied. Comparing serial sections and examining three-dimensional reconstruction of small nerve plexus, it was demonstrated that various varicosities differed only in their correlation of the amount of small (30-80 nm) and large (80-180 nm) vesicles. Average diameters in profiles of small and large vesicles are 56.3 nm and 115.6 nm, respectively. There are varicosities containing about 250 or more than 1,000 vesicles. Evenly distributed vesicles throughout the volume of varicosities and lack of specialized structures on the axolemma are supposed to demonstrate the absence of special areas for the mediator removal in the axons studied. The microtubes in the varicosities are peripherally arranged, next to the axolemma and form 1-1.5 wide coils. A suggestion is made that the varicosities in neighbouring axons of the same nerve plexus, with specific structural organization, are special functional units and appear to be peculiar not only for nerve plexus of the coronary artery, but also for other parts of the peripheral vegetative nerve system.", "contents": "[Quantitative analysis of the structure of the varicose dilatations in the nerve fibers of the rabbit coronary artery (according to the results of three-dimensional reconstruction)]. Axoplasmic vesicles and microtubes in varicosities of axonal plexus in the external sheath of the rabbit coronary artery have been studied. Comparing serial sections and examining three-dimensional reconstruction of small nerve plexus, it was demonstrated that various varicosities differed only in their correlation of the amount of small (30-80 nm) and large (80-180 nm) vesicles. Average diameters in profiles of small and large vesicles are 56.3 nm and 115.6 nm, respectively. There are varicosities containing about 250 or more than 1,000 vesicles. Evenly distributed vesicles throughout the volume of varicosities and lack of specialized structures on the axolemma are supposed to demonstrate the absence of special areas for the mediator removal in the axons studied. The microtubes in the varicosities are peripherally arranged, next to the axolemma and form 1-1.5 wide coils. A suggestion is made that the varicosities in neighbouring axons of the same nerve plexus, with specific structural organization, are special functional units and appear to be peculiar not only for nerve plexus of the coronary artery, but also for other parts of the peripheral vegetative nerve system."}
{"id": "PMID:464829", "title": "[Quantitative characteristics of components of the placental barrier of women with uncomplicated full-term pregnancies].", "content": "Placentas from four healthy women have been studied electron microscopically after urgent spontaneous deliveries. In every placenta 130-200 resorptive villi of the chorion were photographed. The results on morphometric analysis of 1,205 electronograms of the placental barrier are presented. At full-term uncomplicated pregnancies all components of the placental barrier (syncytium, cytotrophoblast, basal membrane of the trophoblast, stromal connective-tissue layer, basal membrane and endothelium of fetal capillaries) have been stated to demonstrate rather stable quantitative characteristics (see Table in the text). The quantitative data obtained can serve for electron microscopic investigation of placenta at different pathologic states of the mother and the fetus, make it possible to differentiate more precisely physiological and pathological changes in placental ultrastructure.", "contents": "[Quantitative characteristics of components of the placental barrier of women with uncomplicated full-term pregnancies]. Placentas from four healthy women have been studied electron microscopically after urgent spontaneous deliveries. In every placenta 130-200 resorptive villi of the chorion were photographed. The results on morphometric analysis of 1,205 electronograms of the placental barrier are presented. At full-term uncomplicated pregnancies all components of the placental barrier (syncytium, cytotrophoblast, basal membrane of the trophoblast, stromal connective-tissue layer, basal membrane and endothelium of fetal capillaries) have been stated to demonstrate rather stable quantitative characteristics (see Table in the text). The quantitative data obtained can serve for electron microscopic investigation of placenta at different pathologic states of the mother and the fetus, make it possible to differentiate more precisely physiological and pathological changes in placental ultrastructure."}
{"id": "PMID:464830", "title": "[Coelomic gonadal epithelia during the period of human embryonic morphologic sex differentiation].", "content": "The course of divergent differentiation of coelomic epithelium in the indifferent gonad has been traced up to the stage when morphological sex signs are distinctly seen. Eleven human embryos fixed in Carnoy and Bouin's fluid have been studied from 6 to 10 weeks of gestation. Paraffin sections were stained with hematoxylin, ferric hematoxylin after Heidenhain. Basal membrane fibres were revealed by silver impregnation after Karupu. PAS-reaction after McManus and additional staining with hematoxylin and light green were applied, as well as the reaction for total proteins with bromphenol blue after Miguel--Calvo. Sex differentiation of coelomic epithelium in the gonad developing according to the male type begins a little later than in the ovary. In the epithelium of the forming testis a great amount of mitotic figures is observed that might be connected with the presence of Y-chromosome stimulating cells for extra mitotic cycles. The increasing presence of Y-chromosome stimulating cells for extra mitotic cycles. The increasing number of mitotic figures in the epithelium should be considered as a sign demonstrating that differentiation according to the male type has started. Superficial epithelium of the embryonic ovary has a peculiar false pseudostratified structure. This secures cellular reserve for the ovarian cortex formation from the external epithelium. The apical surface of the cells in the external epithelium has a border which is evidently formed by microvilli and revealed by PAS-reaction and bromphenol blue. Ovarian follicular cells and Sertoli's cells (sustentocytes) in testis have the common origin in the human fetus--from coelomic epithelium of the gonad germ.", "contents": "[Coelomic gonadal epithelia during the period of human embryonic morphologic sex differentiation]. The course of divergent differentiation of coelomic epithelium in the indifferent gonad has been traced up to the stage when morphological sex signs are distinctly seen. Eleven human embryos fixed in Carnoy and Bouin's fluid have been studied from 6 to 10 weeks of gestation. Paraffin sections were stained with hematoxylin, ferric hematoxylin after Heidenhain. Basal membrane fibres were revealed by silver impregnation after Karupu. PAS-reaction after McManus and additional staining with hematoxylin and light green were applied, as well as the reaction for total proteins with bromphenol blue after Miguel--Calvo. Sex differentiation of coelomic epithelium in the gonad developing according to the male type begins a little later than in the ovary. In the epithelium of the forming testis a great amount of mitotic figures is observed that might be connected with the presence of Y-chromosome stimulating cells for extra mitotic cycles. The increasing presence of Y-chromosome stimulating cells for extra mitotic cycles. The increasing number of mitotic figures in the epithelium should be considered as a sign demonstrating that differentiation according to the male type has started. Superficial epithelium of the embryonic ovary has a peculiar false pseudostratified structure. This secures cellular reserve for the ovarian cortex formation from the external epithelium. The apical surface of the cells in the external epithelium has a border which is evidently formed by microvilli and revealed by PAS-reaction and bromphenol blue. Ovarian follicular cells and Sertoli's cells (sustentocytes) in testis have the common origin in the human fetus--from coelomic epithelium of the gonad germ."}
{"id": "PMID:464831", "title": "[Skeletal muscle ultrastructure of untrained humans and animals after physical loading].", "content": "In non-trained mice subjected to a single and repeated swimming up to complete wearness, fibres in the skeletal muscles demonstrated certain ultrastructural alterations in their nuclei. Twenty-four and 48 h after a single swimming many muscular nuclei have only non-condensed chromatin. After repeated swimming large clumps of condensed chromatin appear in most of the muscular fibre nuclei. These nuclear changes are accompanied by segregation of separate fibrillar areas containing nuclei. Similar phenomena are also noticed in muscular fibres of non-trained people after a single physical loading. Ultrastructural changes in skeletal muscular fibres of volunteers, nonsportsmen are of individual character and depend on the degree of wearness resulting from the loading. In people subjected to the experiment and having severe fatigue (disability to work) significant shifts in submicroscopical structure of the fibrillar components were observed: in mitochondria, sarcoplasmic reticulum, T-system, contractile apparatus, etc. In this case, matrix becomes clear, lysis in mitochondrial crysts, dilatation of terminal cysterns of the sarcoplasmic reticulum and T-system tubes, destructive and regenerative processes in the contractile apparatus are observed. The data obtained demonstrate varied changes in ultrastructure of different components composing skeletal muscles of the non-trained organism subjected to physical loading.", "contents": "[Skeletal muscle ultrastructure of untrained humans and animals after physical loading]. In non-trained mice subjected to a single and repeated swimming up to complete wearness, fibres in the skeletal muscles demonstrated certain ultrastructural alterations in their nuclei. Twenty-four and 48 h after a single swimming many muscular nuclei have only non-condensed chromatin. After repeated swimming large clumps of condensed chromatin appear in most of the muscular fibre nuclei. These nuclear changes are accompanied by segregation of separate fibrillar areas containing nuclei. Similar phenomena are also noticed in muscular fibres of non-trained people after a single physical loading. Ultrastructural changes in skeletal muscular fibres of volunteers, nonsportsmen are of individual character and depend on the degree of wearness resulting from the loading. In people subjected to the experiment and having severe fatigue (disability to work) significant shifts in submicroscopical structure of the fibrillar components were observed: in mitochondria, sarcoplasmic reticulum, T-system, contractile apparatus, etc. In this case, matrix becomes clear, lysis in mitochondrial crysts, dilatation of terminal cysterns of the sarcoplasmic reticulum and T-system tubes, destructive and regenerative processes in the contractile apparatus are observed. The data obtained demonstrate varied changes in ultrastructure of different components composing skeletal muscles of the non-trained organism subjected to physical loading."}
{"id": "PMID:464832", "title": "[Histological classification of stomach cancer and its prognostic value].", "content": "The morphology of stomach carcinoma was analysed in relation to the longevity of the patients after a radical operation for 1958--1967 (513 observations). The highest prognostic importance was found to belong to such factors as the presence of metastases in the perigastral lymph nodes, the depth of the tumour outgrowth through the stomach wall, the pattern of the tumour growth in relation to the surrounding tissue (pushing back or infiltrating), mucus formation in the tumour, the reaction of the tumour stroma and the surrounding tissue in the form of infiltration with lymphoid or plasma cells. The histological form of the tumour by itself as well as the degree of its structural differentiation are of little value for the prognosis. From the foregoing, a histological classification of stomach carcinoma is suggested including the two main histological forms of stomach carcinoma: adenocarcinoma and carcinoma simplex, and reflecting most prognostically important features of the tumour.", "contents": "[Histological classification of stomach cancer and its prognostic value]. The morphology of stomach carcinoma was analysed in relation to the longevity of the patients after a radical operation for 1958--1967 (513 observations). The highest prognostic importance was found to belong to such factors as the presence of metastases in the perigastral lymph nodes, the depth of the tumour outgrowth through the stomach wall, the pattern of the tumour growth in relation to the surrounding tissue (pushing back or infiltrating), mucus formation in the tumour, the reaction of the tumour stroma and the surrounding tissue in the form of infiltration with lymphoid or plasma cells. The histological form of the tumour by itself as well as the degree of its structural differentiation are of little value for the prognosis. From the foregoing, a histological classification of stomach carcinoma is suggested including the two main histological forms of stomach carcinoma: adenocarcinoma and carcinoma simplex, and reflecting most prognostically important features of the tumour."}
{"id": "PMID:464833", "title": "[Benign non-epithelial tumors of the stomach].", "content": "Six cases of mature non-epithelial tumours of the stomach are described; epithelioid leiomyoma, leiomyoma, lipoma, two fibromata and angiofibrolipoma. Noteworthy is the extremely rarely occurring epithelioid leiomyoma. In one case, lipoma and chronic peptic ulcer coexisted. All the stomach tumours under study were mature benign ones, but some of them presented a clinical course with signs of malignancy probably due to the endogastric growth of the tumour, its dimensions, and its localization near the stomach outlet, as well as to the breach of integrity of the mucous membrane above the tumour.", "contents": "[Benign non-epithelial tumors of the stomach]. Six cases of mature non-epithelial tumours of the stomach are described; epithelioid leiomyoma, leiomyoma, lipoma, two fibromata and angiofibrolipoma. Noteworthy is the extremely rarely occurring epithelioid leiomyoma. In one case, lipoma and chronic peptic ulcer coexisted. All the stomach tumours under study were mature benign ones, but some of them presented a clinical course with signs of malignancy probably due to the endogastric growth of the tumour, its dimensions, and its localization near the stomach outlet, as well as to the breach of integrity of the mucous membrane above the tumour."}
{"id": "PMID:464834", "title": "[Morphology of experimental tumors of the sympathetic nervous system].", "content": "Morphology of experimental tumours of the sympathetic nervous system in rabbits and hamsters induced by administration of nitrose compounds and the use of modifying factors was studied. The tumours were localized in the mediastinum, retroperitoneal space, adrenals and kidneys. The predominant involvement of the female animals was observed. Histological, histochemical and electron microscopic study of 33 tumours of the sympathetic nervous system was carried out. The tumours were classified as ganglioneuromas, ganglioneuroblastomas and sympathoblastomas by their degree of maturity. The experimental tumours by their structure were similar to the analogous neoplasias of man.", "contents": "[Morphology of experimental tumors of the sympathetic nervous system]. Morphology of experimental tumours of the sympathetic nervous system in rabbits and hamsters induced by administration of nitrose compounds and the use of modifying factors was studied. The tumours were localized in the mediastinum, retroperitoneal space, adrenals and kidneys. The predominant involvement of the female animals was observed. Histological, histochemical and electron microscopic study of 33 tumours of the sympathetic nervous system was carried out. The tumours were classified as ganglioneuromas, ganglioneuroblastomas and sympathoblastomas by their degree of maturity. The experimental tumours by their structure were similar to the analogous neoplasias of man."}
{"id": "PMID:464835", "title": "[Correlation analysis of the organometric heart parameters under normal and pathological conditions].", "content": "An organometric study of 27 heart parameters normally, in hypertension, chronic nonspecific pulmonary diseases and ischemic heart disease was carried out. Significant differences in the heart weight values under normal and pathological conditions were found. A correlation analysis of the heart parameters normally and pathologically was performed revealing specific associations for each group of pathology. Increased associations were observed between heart weight values and its parts and the linear-planimetric parameters in the groups of ischemic heart disease and chronic nonspecific pulmonary diseases.", "contents": "[Correlation analysis of the organometric heart parameters under normal and pathological conditions]. An organometric study of 27 heart parameters normally, in hypertension, chronic nonspecific pulmonary diseases and ischemic heart disease was carried out. Significant differences in the heart weight values under normal and pathological conditions were found. A correlation analysis of the heart parameters normally and pathologically was performed revealing specific associations for each group of pathology. Increased associations were observed between heart weight values and its parts and the linear-planimetric parameters in the groups of ischemic heart disease and chronic nonspecific pulmonary diseases."}
{"id": "PMID:464836", "title": "[Morphological characteristics of acute viral hepatitis].", "content": "From the literature and their own observations the authors present morphological criteria of acute viral hepatitis which may be used for differential diagnosis from hepatites of other etiology (toxic, drug). Examinations of liver biopsies from patients with viral hepatitis permit to establish the stage of the disease and to predict its possible outcomes.", "contents": "[Morphological characteristics of acute viral hepatitis]. From the literature and their own observations the authors present morphological criteria of acute viral hepatitis which may be used for differential diagnosis from hepatites of other etiology (toxic, drug). Examinations of liver biopsies from patients with viral hepatitis permit to establish the stage of the disease and to predict its possible outcomes."}
{"id": "PMID:464837", "title": "[Use of morphometry in pathological anatomy].", "content": "The paper describes the main principles of morphometry and its concepts and characterizes the potentials of the morphometric methods. Statistical sampling and conditions of preserving its representative character are dealt with. The implications of some concepts of variation statistics are analysed: mean values, variability indices, confidence intervals, thresholds of confidence probability, criteria of significance, regularities of the distribution of frequencies of sampling elements. The main rules of the factor, correlation, regression, dispersion, and information analysis are described. Attention is drawn to the insufficient use of morphometry potentials. It is stated that morphometric description should not overshadow the specific features of the structures under study.", "contents": "[Use of morphometry in pathological anatomy]. The paper describes the main principles of morphometry and its concepts and characterizes the potentials of the morphometric methods. Statistical sampling and conditions of preserving its representative character are dealt with. The implications of some concepts of variation statistics are analysed: mean values, variability indices, confidence intervals, thresholds of confidence probability, criteria of significance, regularities of the distribution of frequencies of sampling elements. The main rules of the factor, correlation, regression, dispersion, and information analysis are described. Attention is drawn to the insufficient use of morphometry potentials. It is stated that morphometric description should not overshadow the specific features of the structures under study."}
{"id": "PMID:464839", "title": "[2 cases of  hemangioendothelioma of the liver and the spleen].", "content": "Hemangioendothelioma of the liver and spleen with similar affection of the organs resembling a bloody sponge was diagnosed histologically in men of 35 and 59 years. Microscopically, the structure of the tumour presented a picture of multiple ugly vascular fissures and cavities lined with atypical polymorphous endothelial cells not infrequently transformed into giant cells with hyperchronic nuclei. Impregnation of the sections with silver nitrate according to Gomori revealed an irregular vascular network and facilitated the recognition of the true nature of the disease.", "contents": "[2 cases of  hemangioendothelioma of the liver and the spleen]. Hemangioendothelioma of the liver and spleen with similar affection of the organs resembling a bloody sponge was diagnosed histologically in men of 35 and 59 years. Microscopically, the structure of the tumour presented a picture of multiple ugly vascular fissures and cavities lined with atypical polymorphous endothelial cells not infrequently transformed into giant cells with hyperchronic nuclei. Impregnation of the sections with silver nitrate according to Gomori revealed an irregular vascular network and facilitated the recognition of the true nature of the disease."}
{"id": "PMID:464840", "title": "[Malignant carcinoid tumor of the bladder].", "content": "One of the first observations of malignant carcinoid of the urinary bladder in a man of 42 is described. Histologically the tumour resembled solid-alveolar transitional cell carcinoma. The carcinoid was verified by finding in the tumour cells electron dense endocrine granules and helix-like rough endoplasmic reticulum. Possible sources of urothelium carcinoid development are discussed.", "contents": "[Malignant carcinoid tumor of the bladder]. One of the first observations of malignant carcinoid of the urinary bladder in a man of 42 is described. Histologically the tumour resembled solid-alveolar transitional cell carcinoma. The carcinoid was verified by finding in the tumour cells electron dense endocrine granules and helix-like rough endoplasmic reticulum. Possible sources of urothelium carcinoid development are discussed."}
{"id": "PMID:464841", "title": "[Oleogranulomatosis of the lung as a late complication of oleothorax in a patient with pulmonary tuberculosis].", "content": "An observation of total oleogranulomatosis of the lung developing in a patient as a rare complication of extrapleural oleothorax used for treatment of infiltrative pulmonary tuberculosis is described.", "contents": "[Oleogranulomatosis of the lung as a late complication of oleothorax in a patient with pulmonary tuberculosis]. An observation of total oleogranulomatosis of the lung developing in a patient as a rare complication of extrapleural oleothorax used for treatment of infiltrative pulmonary tuberculosis is described."}
{"id": "PMID:464842", "title": "[Generalized sarcoidosis with the involvement of the brain].", "content": "A rare case of generalized sarcoidosis with the involvement of the brain is presented. Death occurred as a result of the sarcoid affection of the central nervous system, granulomatous vasculitis of the brain meninges and substance.", "contents": "[Generalized sarcoidosis with the involvement of the brain]. A rare case of generalized sarcoidosis with the involvement of the brain is presented. Death occurred as a result of the sarcoid affection of the central nervous system, granulomatous vasculitis of the brain meninges and substance."}
{"id": "PMID:464859", "title": "[Insecticide poisoning. Microdissection of nerves and muscle histochemistry in 10 cases].", "content": "A sural nerve and a muscle biopsy study of patients with chronic insecticides poisoning, with teased fiber preparations, routine pathologic studies of nerves and histochemistry of muscle is reported. The sural nerves of ten patients were studied and a teased fiber preparation was done in nine. The tenth patient had only fibrosis and no myelin was found. The sural nerves were abnormal in all patients and the teased fiber preparation resulted in preponderance of type C, D and large amount of G type fibers, according to Dyck's classification. These fibers had enlargement of the axon and myelin sheath, also seen in routine sections. The muscle biopsy with routine and histochemistry methods was done in 8 cases; in 6 there was found signs of denervation; the remaining cases were normal, but these were proximal muscles. The authors conclude that the process primarily interfere with the functions of the axons, with distal axonal degeneration and a dying back phenomen.", "contents": "[Insecticide poisoning. Microdissection of nerves and muscle histochemistry in 10 cases]. A sural nerve and a muscle biopsy study of patients with chronic insecticides poisoning, with teased fiber preparations, routine pathologic studies of nerves and histochemistry of muscle is reported. The sural nerves of ten patients were studied and a teased fiber preparation was done in nine. The tenth patient had only fibrosis and no myelin was found. The sural nerves were abnormal in all patients and the teased fiber preparation resulted in preponderance of type C, D and large amount of G type fibers, according to Dyck's classification. These fibers had enlargement of the axon and myelin sheath, also seen in routine sections. The muscle biopsy with routine and histochemistry methods was done in 8 cases; in 6 there was found signs of denervation; the remaining cases were normal, but these were proximal muscles. The authors conclude that the process primarily interfere with the functions of the axons, with distal axonal degeneration and a dying back phenomen."}
{"id": "PMID:464860", "title": "[Involvement of the central nervous system in cysticercosis].", "content": "The involvement of the central nervous system in cysticercosis was demonstrate as an incidental finding in a frequence of 0,3% in 4000 autopsied cases. Most of the 12 patients came from rural areas of the State of Bahia. The low frequence compared to other sites reported in Brasil shows that the neurcysticercosis does not represent an important problem in Bahia. The host tissue response observed was represented by a chronic fibrosing inflammatory type of reaction. This study points out the importance of the methenamine silver stain (Grocott) in the identification of the degenerated embryo inside the fibrous and/or calcified lesions.", "contents": "[Involvement of the central nervous system in cysticercosis]. The involvement of the central nervous system in cysticercosis was demonstrate as an incidental finding in a frequence of 0,3% in 4000 autopsied cases. Most of the 12 patients came from rural areas of the State of Bahia. The low frequence compared to other sites reported in Brasil shows that the neurcysticercosis does not represent an important problem in Bahia. The host tissue response observed was represented by a chronic fibrosing inflammatory type of reaction. This study points out the importance of the methenamine silver stain (Grocott) in the identification of the degenerated embryo inside the fibrous and/or calcified lesions."}
{"id": "PMID:464861", "title": "[Prophylactic antibiotics in neurosurgery].", "content": "The post-operative infection-rate in neurosurgical patients who received prophylactic antibiotics was compared to the infection-rate of patients who received no antibiotics. None of the 73 studied patients had pre-operative infection. Infections occured in 26,4% of the patients in the first group and in none of the second group (p less than 0,2); 27,5% of the patients with intracranial lesions and 9% of the patients with spinal lesions in the frist group had post-operative infections, and none in the second group (p less than 0,05 and 0.05 less than p less than 0.1). 67% of all the patients had severe neurological lesions; 27% of these had post-operative infections; only 4% of the patients with mild lesions had infections (p less than 0.05). Post-operative infections were severe and fatal in most of these cases. Prophylactic broad-spectrum antibiotics were of no value in preventing post-operative infections in these neurosurgical patients and those who received no antibiotics had a significantly lower rate of infections.", "contents": "[Prophylactic antibiotics in neurosurgery]. The post-operative infection-rate in neurosurgical patients who received prophylactic antibiotics was compared to the infection-rate of patients who received no antibiotics. None of the 73 studied patients had pre-operative infection. Infections occured in 26,4% of the patients in the first group and in none of the second group (p less than 0,2); 27,5% of the patients with intracranial lesions and 9% of the patients with spinal lesions in the frist group had post-operative infections, and none in the second group (p less than 0,05 and 0.05 less than p less than 0.1). 67% of all the patients had severe neurological lesions; 27% of these had post-operative infections; only 4% of the patients with mild lesions had infections (p less than 0.05). Post-operative infections were severe and fatal in most of these cases. Prophylactic broad-spectrum antibiotics were of no value in preventing post-operative infections in these neurosurgical patients and those who received no antibiotics had a significantly lower rate of infections."}
{"id": "PMID:464862", "title": "[Adult form of Niemann-Pick disease associated with Osler-Rendu-Weber syndrome. Report of a case].", "content": "The case of a 28-year-old female with an adult form of Niemann-Pick disease is described. The illness started at the age of 22 years. The characteristics actually established in the adult form of this lipidosis were discussed. Vision was impaired but there was no clinical evidence of importnat visual involvement. Neurologic and psychiatric examinations were always normal. The bone marrow contained foam cells. A cherry red spot was present in the macula. An uncommon association with Osler-Rendu-Weber disease was described. The patient maintained normal clinical and neurological conditions, that is, she continues to show an extraordinarily benign course with poor cerebral participation represented by a moderate localized cortical atrophy seen at the pneumoencephalogram.", "contents": "[Adult form of Niemann-Pick disease associated with Osler-Rendu-Weber syndrome. Report of a case]. The case of a 28-year-old female with an adult form of Niemann-Pick disease is described. The illness started at the age of 22 years. The characteristics actually established in the adult form of this lipidosis were discussed. Vision was impaired but there was no clinical evidence of importnat visual involvement. Neurologic and psychiatric examinations were always normal. The bone marrow contained foam cells. A cherry red spot was present in the macula. An uncommon association with Osler-Rendu-Weber disease was described. The patient maintained normal clinical and neurological conditions, that is, she continues to show an extraordinarily benign course with poor cerebral participation represented by a moderate localized cortical atrophy seen at the pneumoencephalogram."}
{"id": "PMID:464863", "title": "[Transverse myelopathy and systemic lupus erythematosus. Report of a case and review of the literature].", "content": "A case of transverse myelopathy in systemic lupus erythematosus with subacute onset and fatal course is reported. A review of the literature is done and clinical, laboratorial, therapeutics and pathological aspects are discussed. The authors call attention to the poor results of therapy and discuss the participation of immunological factors leaving to demyelination which are, aside the classical vascular involvement, the probable mechanisms in the neurological manifestations of systemic lupus erythematosus. The authors believe that, in their case, such a demyelinative mechanism was responsible for the clinical picture, although a necroscopic examination was not allowed.", "contents": "[Transverse myelopathy and systemic lupus erythematosus. Report of a case and review of the literature]. A case of transverse myelopathy in systemic lupus erythematosus with subacute onset and fatal course is reported. A review of the literature is done and clinical, laboratorial, therapeutics and pathological aspects are discussed. The authors call attention to the poor results of therapy and discuss the participation of immunological factors leaving to demyelination which are, aside the classical vascular involvement, the probable mechanisms in the neurological manifestations of systemic lupus erythematosus. The authors believe that, in their case, such a demyelinative mechanism was responsible for the clinical picture, although a necroscopic examination was not allowed."}
{"id": "PMID:464864", "title": "[Diabetes insipidus after myelography. Report of a case].", "content": "The case of a 28 year-old man in whom diabetes insipidus appeared 24 hours after Pantopaque myelography is reported. This unusual complication, not previously reported, probably was caused by a hypersensitivity reaction or mechanical-irritative effect on the hypothalamic-hipophyseal area produced by suprasellar Pantopaque droplets, demonstrated by skull x-rays. The patient improved after being treated with steroids. Some serious complications of myelography can be prevented by avoiding supratentorial spillage of Pantopaque.", "contents": "[Diabetes insipidus after myelography. Report of a case]. The case of a 28 year-old man in whom diabetes insipidus appeared 24 hours after Pantopaque myelography is reported. This unusual complication, not previously reported, probably was caused by a hypersensitivity reaction or mechanical-irritative effect on the hypothalamic-hipophyseal area produced by suprasellar Pantopaque droplets, demonstrated by skull x-rays. The patient improved after being treated with steroids. Some serious complications of myelography can be prevented by avoiding supratentorial spillage of Pantopaque."}
{"id": "PMID:464866", "title": "Optic disc parameters and onset of glaucomatous field loss. I. Methods and progressive changes in disc morphology.", "content": "Serial stereoscopic fundus photographs taken in known relationship to the onset of glaucomatous visual field loss on 12 eyes were intermixed with those from 206 age- and race-matched controls and analyzed in randomized masked fashion. Progressive changes in the size, shape, or contour of the disc, and a newly described parameter, thickness of the nerve fiber layer as it crosses the disc rim, were readily apparent by the time of onset of glaucomatous field loss in all but two abnormal eyes (one case). In the latter instance, direct comparison of stereophotos indicated progressive pallor of the remaining disc tissue. Serial stereophotographs appear superior to fundus drawings for anticipating glaucomatous field loss.", "contents": "Optic disc parameters and onset of glaucomatous field loss. I. Methods and progressive changes in disc morphology. Serial stereoscopic fundus photographs taken in known relationship to the onset of glaucomatous visual field loss on 12 eyes were intermixed with those from 206 age- and race-matched controls and analyzed in randomized masked fashion. Progressive changes in the size, shape, or contour of the disc, and a newly described parameter, thickness of the nerve fiber layer as it crosses the disc rim, were readily apparent by the time of onset of glaucomatous field loss in all but two abnormal eyes (one case). In the latter instance, direct comparison of stereophotos indicated progressive pallor of the remaining disc tissue. Serial stereophotographs appear superior to fundus drawings for anticipating glaucomatous field loss."}
{"id": "PMID:464867", "title": "Optic disc parameters and onset of glaucomatous field loss. II. Static screening criteria.", "content": "Stereoscopic fundus photographs of 17 abnormal eyes, taken in known temporal relationship to the onset of glaucomatous visual field loss, and 206 eyes of age- and race-matched controls were examined in randomized masked fashion. Width of the narrowest remaining disc rim, size of the vertical and horizontal physical cups, contour of the temporal and nasal slopes (vertical ovalness), and a newly described parameter, thickness of the nerve fiber layer as it crosses the disc rim, were all useful for distinguishing patients with impending or established visual field loss. None, however, was as effective as defects in the nerve fiber layer, the only parameter displaying sufficient promise as a clinical screening tool to warrant initiation of large-scale prospective evaluation.", "contents": "Optic disc parameters and onset of glaucomatous field loss. II. Static screening criteria. Stereoscopic fundus photographs of 17 abnormal eyes, taken in known temporal relationship to the onset of glaucomatous visual field loss, and 206 eyes of age- and race-matched controls were examined in randomized masked fashion. Width of the narrowest remaining disc rim, size of the vertical and horizontal physical cups, contour of the temporal and nasal slopes (vertical ovalness), and a newly described parameter, thickness of the nerve fiber layer as it crosses the disc rim, were all useful for distinguishing patients with impending or established visual field loss. None, however, was as effective as defects in the nerve fiber layer, the only parameter displaying sufficient promise as a clinical screening tool to warrant initiation of large-scale prospective evaluation."}
{"id": "PMID:464868", "title": "Multivariate analysis of the risk of glaucomatous visual field loss.", "content": "In a retrospective study, 92 patients with ocular hypertension, ie, intraocular pressure of 21 mm Hg or higher, and no evidence of glaucomatous visual field defects, were observed for five years. Visual field defects developed in one or both eyes of 33 patients during the five-year follow-up period, while none were detected in the remaining 59. Values for suspected risk factors, determined at the outset of the follow-up period, were subjected to a multivariate analysis with use of linear discriminant analysis and a multiple logistic function. Models of risk providing maximum separation of the two patient groups (visual field loss vs no visual field loss) found that the risk factors having the greatest significance for prediction of visual field loss included vertical estimates of cup/disc ratio, mean IOP during the period of observation, a positive family history of glaucoma, and age. Factors having the lowest predictive values included IOP response to topical dexamethasone, plasma cortisol suppression, and a history of systemic hypertension.", "contents": "Multivariate analysis of the risk of glaucomatous visual field loss. In a retrospective study, 92 patients with ocular hypertension, ie, intraocular pressure of 21 mm Hg or higher, and no evidence of glaucomatous visual field defects, were observed for five years. Visual field defects developed in one or both eyes of 33 patients during the five-year follow-up period, while none were detected in the remaining 59. Values for suspected risk factors, determined at the outset of the follow-up period, were subjected to a multivariate analysis with use of linear discriminant analysis and a multiple logistic function. Models of risk providing maximum separation of the two patient groups (visual field loss vs no visual field loss) found that the risk factors having the greatest significance for prediction of visual field loss included vertical estimates of cup/disc ratio, mean IOP during the period of observation, a positive family history of glaucoma, and age. Factors having the lowest predictive values included IOP response to topical dexamethasone, plasma cortisol suppression, and a history of systemic hypertension."}
{"id": "PMID:464869", "title": "Optic nerve decompression. Presumed postoperative development of medullated nerve fibers.", "content": "A child had an inoperable arterial venous malformation with chronic papilledema first noticed at age 7 months. Bilateral optic nerve decompressions were performed with satisfactory results. What appeared to be medullated nerve fibers developed in the retina of one eye 5 1/2 months after surgery.", "contents": "Optic nerve decompression. Presumed postoperative development of medullated nerve fibers. A child had an inoperable arterial venous malformation with chronic papilledema first noticed at age 7 months. Bilateral optic nerve decompressions were performed with satisfactory results. What appeared to be medullated nerve fibers developed in the retina of one eye 5 1/2 months after surgery."}
{"id": "PMID:464870", "title": "Comparison of photo slit lamp and fundus camera photography of the optic disc.", "content": "The photo slit lamp has characteristics that may be important in optic disc photography for measuring glaucomatous cupping. This finding led to the question of whether disc photographs with the slit lamp showed less detail than fundus camera photographs. Tests were conducted on in-air resolution and of each instrument's ability to record fine vessel detail in normal human optic discs. The results showed that the instruments performed comparably in each test.", "contents": "Comparison of photo slit lamp and fundus camera photography of the optic disc. The photo slit lamp has characteristics that may be important in optic disc photography for measuring glaucomatous cupping. This finding led to the question of whether disc photographs with the slit lamp showed less detail than fundus camera photographs. Tests were conducted on in-air resolution and of each instrument's ability to record fine vessel detail in normal human optic discs. The results showed that the instruments performed comparably in each test."}
{"id": "PMID:464871", "title": "Ultrasonographic features of choroidal malignant melanomas.", "content": "In a 12-month period 26 eyes were enucleated after an ultrasonographic diagnosis was made of malignant melanoma of the choroid. Histologic features of the tumors were reviewed. The ultrasonographic diagnosis was correct in 100% (26/26) of enucleated eyes. Spontaneous vascular movement (SVM) was identified by standardized A-scan echography in 69% (18/26) of the tumors. Contact B-scan ultrasonography demonstrated choroidal excavation in 65% (17/26) of the melanomas. During the 12-month study period, choroidal excavation was also demonstrated in cases of metastatic choroidal tumors, hemangiomas of the choroid, and long-standing choroidal nevi. The standardized A-scan finding of SVM is a compelling sign in the diagnosis of choroidal malignant melanoma. In contrast, the B-scan finding of choroidal excavation is a less useful sign since it may also be seen in various other ocular lesions.", "contents": "Ultrasonographic features of choroidal malignant melanomas. In a 12-month period 26 eyes were enucleated after an ultrasonographic diagnosis was made of malignant melanoma of the choroid. Histologic features of the tumors were reviewed. The ultrasonographic diagnosis was correct in 100% (26/26) of enucleated eyes. Spontaneous vascular movement (SVM) was identified by standardized A-scan echography in 69% (18/26) of the tumors. Contact B-scan ultrasonography demonstrated choroidal excavation in 65% (17/26) of the melanomas. During the 12-month study period, choroidal excavation was also demonstrated in cases of metastatic choroidal tumors, hemangiomas of the choroid, and long-standing choroidal nevi. The standardized A-scan finding of SVM is a compelling sign in the diagnosis of choroidal malignant melanoma. In contrast, the B-scan finding of choroidal excavation is a less useful sign since it may also be seen in various other ocular lesions."}
{"id": "PMID:464872", "title": "Use of air to decrease endothelial cell loss during intraocular lens implantation.", "content": "We performed 26 consecutive intracapsular cataract extractions with lens implantation. The last 17 implantations were performed with air instilled in the anterior chamber, whereas the first nine operations were performed without air. The central corneal endothelium of each eye operated on was photographed before and within five days after lens implantation. A computer analysis of the endothelial photographs showed that, on the average, 32% of the central corneal endothelial cells were lost during lens implantation without the use of air and 15% were lost when the lens was implanted with air. Increased endothelial cell loss was significantly correlated with the failure to implant the lens with use of air, with increased operative endothelial trauma, and with higher postoperative intraocular pressures. A significantly larger increase in corneal thickness on the first postoperative day occurred in patients with larger or more varied cell sizes preoperatively.", "contents": "Use of air to decrease endothelial cell loss during intraocular lens implantation. We performed 26 consecutive intracapsular cataract extractions with lens implantation. The last 17 implantations were performed with air instilled in the anterior chamber, whereas the first nine operations were performed without air. The central corneal endothelium of each eye operated on was photographed before and within five days after lens implantation. A computer analysis of the endothelial photographs showed that, on the average, 32% of the central corneal endothelial cells were lost during lens implantation without the use of air and 15% were lost when the lens was implanted with air. Increased endothelial cell loss was significantly correlated with the failure to implant the lens with use of air, with increased operative endothelial trauma, and with higher postoperative intraocular pressures. A significantly larger increase in corneal thickness on the first postoperative day occurred in patients with larger or more varied cell sizes preoperatively."}
{"id": "PMID:464873", "title": "Posterior scleritis. A cause of diagnostic confusion.", "content": "The referring diagnoses in seven women with posterior scleritis included intraocular neoplasm, retrobulbar tumor, choroiditis, and idiopathic central serous choroidopathy. In all cases, a localized area of intense posterior scleritis was responsible for the misdiagnosed ocular findings. Features that helped to correctly identify posterior scleritis were as follows: female sex; a history of anterior scleritis; a fundus mass the same color as normal adjacent pigment epithelium; choroidal folds; serous retinal detachment with cloudy fluid; early pinpoint leaking spots from fluorescein angiography; and thickening of the posterior coats of the eye, retrobulbar edema, and high internal reflectivity on ultrasonography. Corticosteroids given for retrobulbar or systemic effect provided effective treatment.", "contents": "Posterior scleritis. A cause of diagnostic confusion. The referring diagnoses in seven women with posterior scleritis included intraocular neoplasm, retrobulbar tumor, choroiditis, and idiopathic central serous choroidopathy. In all cases, a localized area of intense posterior scleritis was responsible for the misdiagnosed ocular findings. Features that helped to correctly identify posterior scleritis were as follows: female sex; a history of anterior scleritis; a fundus mass the same color as normal adjacent pigment epithelium; choroidal folds; serous retinal detachment with cloudy fluid; early pinpoint leaking spots from fluorescein angiography; and thickening of the posterior coats of the eye, retrobulbar edema, and high internal reflectivity on ultrasonography. Corticosteroids given for retrobulbar or systemic effect provided effective treatment."}
{"id": "PMID:464874", "title": "M\u00fcller's muscle excision and levator recession in retracted upper lid. Treatment of thyroid-related retraction.", "content": "The results of excision of M\u00fcller's muscle with or without recession of the levator palpebrae superioris aponeurosis were examined in 61 upper eyelids of 40 patients. The cosmetic results were acceptable in 35 patients (56 upper eyelids). Another five patients had a subsequent unilateral partial (nasal) or total ptosis, which was successfully treated with additional surgery. Overcorrections (ptosis) have ceased to be a problem since modifications to the original technique have been used. Only one undercorrection was encountered, and it was treated successfully with a simple surgical procedure. We believe that the described procedure is desirable for the treatment of thyroid-related upper eyelid retraction because it is simple, is based on anatomic and physiologic principles, is tailored to the individual patient intraoperatively and yields consistently good results.", "contents": "M\u00fcller's muscle excision and levator recession in retracted upper lid. Treatment of thyroid-related retraction. The results of excision of M\u00fcller's muscle with or without recession of the levator palpebrae superioris aponeurosis were examined in 61 upper eyelids of 40 patients. The cosmetic results were acceptable in 35 patients (56 upper eyelids). Another five patients had a subsequent unilateral partial (nasal) or total ptosis, which was successfully treated with additional surgery. Overcorrections (ptosis) have ceased to be a problem since modifications to the original technique have been used. Only one undercorrection was encountered, and it was treated successfully with a simple surgical procedure. We believe that the described procedure is desirable for the treatment of thyroid-related upper eyelid retraction because it is simple, is based on anatomic and physiologic principles, is tailored to the individual patient intraoperatively and yields consistently good results."}
{"id": "PMID:464875", "title": "Vitamin E in kitten oxygen-induced retinopathy. II. Blockage of vitreal neovascularization.", "content": "The effect of vitamin E (tocopherol) on oxygen-induced retinopathy was studied in the kitten after a quantitative scoring system was developed for their India ink injected retinal flat preparations. In 75 previously described kittens exposed to two or three days of oxygen from day 3, treatment from day 1 with vitamin E or placebo disclosed that kittens treated with vitamin E had less retinopathy. Theories of the mechanism of action of vitamin E would predict that, if given only after the oxygen exposure, vitamin E should be ineffective. This was tested in 37 kittens with placebo or drug begun only after withdrawal from oxygen. Unexpectedly, significantly less intravitreal neovascularization was found in kittens treated with vitamin E after oxygen exposure.", "contents": "Vitamin E in kitten oxygen-induced retinopathy. II. Blockage of vitreal neovascularization. The effect of vitamin E (tocopherol) on oxygen-induced retinopathy was studied in the kitten after a quantitative scoring system was developed for their India ink injected retinal flat preparations. In 75 previously described kittens exposed to two or three days of oxygen from day 3, treatment from day 1 with vitamin E or placebo disclosed that kittens treated with vitamin E had less retinopathy. Theories of the mechanism of action of vitamin E would predict that, if given only after the oxygen exposure, vitamin E should be ineffective. This was tested in 37 kittens with placebo or drug begun only after withdrawal from oxygen. Unexpectedly, significantly less intravitreal neovascularization was found in kittens treated with vitamin E after oxygen exposure."}
{"id": "PMID:464882", "title": "Extratemporal facial nerve grafting and radiotherapy.", "content": "Nineteen patients with extratemporal facial nerve grafting procedures and 13 patients with facial hypoglossal anastomosis were followed up with serial photographs for at least one year. The photographic analysis of the results demonstrates that radiotherapy had a detrimental influence on the return of facial movements after extratemporal facial nerve grafting.", "contents": "Extratemporal facial nerve grafting and radiotherapy. Nineteen patients with extratemporal facial nerve grafting procedures and 13 patients with facial hypoglossal anastomosis were followed up with serial photographs for at least one year. The photographic analysis of the results demonstrates that radiotherapy had a detrimental influence on the return of facial movements after extratemporal facial nerve grafting."}
{"id": "PMID:464883", "title": "Prospective tympanometry in 3-year-old children. A study of the spontaneous course of tympanometry types in a nonselected population.", "content": "For six months we observed all 3-year-old children showing type B or C tympanograms at a prevalence study in a geographically limited area (372 ears or 37.2% of the screened total) to study the spontaneous course of middle ear effusions. A considerable test-retest stability was found only for type B. In type C, such stability was equally rare whether the middle ear pressure was highly or only slightly negative. Conversion to type A was observed in about 70% of cases. Ears having a middle ear pressure from -100 to -199 mm H2O almost always returned to normal. The tendency for a negative middle ear pressure or middle ear effusion to develop was the same for both sexes. Conversely, the prognosis of an effusion, once formed, differed significantly, with only girls showing a brief course. Accordingly, a sex-differentiated evaluation appears to be needed in preschool tympanometric screening, and should be included in all clinical considerations when middle ear effusion is demonstrated in young children.", "contents": "Prospective tympanometry in 3-year-old children. A study of the spontaneous course of tympanometry types in a nonselected population. For six months we observed all 3-year-old children showing type B or C tympanograms at a prevalence study in a geographically limited area (372 ears or 37.2% of the screened total) to study the spontaneous course of middle ear effusions. A considerable test-retest stability was found only for type B. In type C, such stability was equally rare whether the middle ear pressure was highly or only slightly negative. Conversion to type A was observed in about 70% of cases. Ears having a middle ear pressure from -100 to -199 mm H2O almost always returned to normal. The tendency for a negative middle ear pressure or middle ear effusion to develop was the same for both sexes. Conversely, the prognosis of an effusion, once formed, differed significantly, with only girls showing a brief course. Accordingly, a sex-differentiated evaluation appears to be needed in preschool tympanometric screening, and should be included in all clinical considerations when middle ear effusion is demonstrated in young children."}
{"id": "PMID:464884", "title": "Effects of pentobaribtal and ketamine on brain stem auditory potentials. Latency and amplitude intensity functions after intraperitoneal administration.", "content": "Latency and amplitude intensity functions of two short latency (less than 5 ms) components (I and IV) of the brain stem auditory potentials were recorded from scalp electrodes in 18 paralyzed, hooded rats breathing with the assistance of artificial respirators. Averaged responses were obtained in three groups of rats before and 30 minutes after intraperitoneal injections of saline, ketamine hydrochloride (Ketalar), and pentobarbital sodium. Although both ketamine and pentobarbital significantly decreased heart rate and altered the gross EEG, they did not significantly change the latency or amplitude intensity functions of the two evoked-potential components.", "contents": "Effects of pentobaribtal and ketamine on brain stem auditory potentials. Latency and amplitude intensity functions after intraperitoneal administration. Latency and amplitude intensity functions of two short latency (less than 5 ms) components (I and IV) of the brain stem auditory potentials were recorded from scalp electrodes in 18 paralyzed, hooded rats breathing with the assistance of artificial respirators. Averaged responses were obtained in three groups of rats before and 30 minutes after intraperitoneal injections of saline, ketamine hydrochloride (Ketalar), and pentobarbital sodium. Although both ketamine and pentobarbital significantly decreased heart rate and altered the gross EEG, they did not significantly change the latency or amplitude intensity functions of the two evoked-potential components."}
{"id": "PMID:464885", "title": "Management of advanced and recurrent facial carcinoma.", "content": "I report five cases of advanced and recurrent facial carcinoma to illustrate the extent to which such disease can develop and yet be compatible with life. Radical treatment approaches are used so as to maximize the chances for tumor ablation and survival as well as for restoration of function and cosmetics.", "contents": "Management of advanced and recurrent facial carcinoma. I report five cases of advanced and recurrent facial carcinoma to illustrate the extent to which such disease can develop and yet be compatible with life. Radical treatment approaches are used so as to maximize the chances for tumor ablation and survival as well as for restoration of function and cosmetics."}
{"id": "PMID:464886", "title": "Electrocochleography of ears with mumps deafness.", "content": "Electrocochleography was performed on 16 affected ears of 15 deaf patients whose deafness had resulted from the mumps. Although pure-tone audiometry showed no response in each case, the cases of mumps deafness can be classified into the following three types of cochlear impairment according to the cochlear microphonic (CM) response: (1) no action potential (AP) response but a well-developed CM response, with impairment at the neural level and probable functioning of Corti's organ; (2) absence of both the AP CM responses, with severe impairment of both the neural regions and Corti's organ; (3) no AP response but a decreased CM response, with severe impairment of the neural regions and partial impairment of Corti's organ.", "contents": "Electrocochleography of ears with mumps deafness. Electrocochleography was performed on 16 affected ears of 15 deaf patients whose deafness had resulted from the mumps. Although pure-tone audiometry showed no response in each case, the cases of mumps deafness can be classified into the following three types of cochlear impairment according to the cochlear microphonic (CM) response: (1) no action potential (AP) response but a well-developed CM response, with impairment at the neural level and probable functioning of Corti's organ; (2) absence of both the AP CM responses, with severe impairment of both the neural regions and Corti's organ; (3) no AP response but a decreased CM response, with severe impairment of the neural regions and partial impairment of Corti's organ."}
{"id": "PMID:464887", "title": "Squamous cell carcinoma of the tonsil and tongue-base region.", "content": "From 1969 through 1975, 145 patients were treated for squamous cell carcinoma of the tonsil and tongue-base region; 119 received initial treatment, and salvage operations were done in 26. The overall five-year survival rate was 42%. Cervical metastasis was the most important determinant of survival. Pathologic stage I or II disease was controlled by surgical treatment. In patients with stage III or IV disease, operation alone controlled the primary lesion better than radiation alone or combined preoperative radiation and surgical treatment. With operation alone, however the rate of neck recurrence was higher than with the other two methods of treatment. In advanced disease, surgical treatment combined with postoperative radiation should be considered. Mandibular osteotomy and excision of the primary lesion are as effective in local tumor control as composite resection. In patients with a tumor-free margin, osteotomy can be used to preserve the mandible.", "contents": "Squamous cell carcinoma of the tonsil and tongue-base region. From 1969 through 1975, 145 patients were treated for squamous cell carcinoma of the tonsil and tongue-base region; 119 received initial treatment, and salvage operations were done in 26. The overall five-year survival rate was 42%. Cervical metastasis was the most important determinant of survival. Pathologic stage I or II disease was controlled by surgical treatment. In patients with stage III or IV disease, operation alone controlled the primary lesion better than radiation alone or combined preoperative radiation and surgical treatment. With operation alone, however the rate of neck recurrence was higher than with the other two methods of treatment. In advanced disease, surgical treatment combined with postoperative radiation should be considered. Mandibular osteotomy and excision of the primary lesion are as effective in local tumor control as composite resection. In patients with a tumor-free margin, osteotomy can be used to preserve the mandible."}
{"id": "PMID:464888", "title": "Vestibular sensory epithelium in Meniere's disease.", "content": "Utricle and horizontal semicircular canal ampulla removed during labyrinthectomy from 11 patients with advanced Meniere's disease were studied under light and electron microscope. In light microscopy the epithelium of both sensory areas appeared fairly normal. Many sensory cells revealed fluid-filled crescents at the nucleus and many cells were extensively vacuolated. Ultrastructurally, a part of the observations were apparently artifacts due to specimen handling; some were normal, age-related findings. Some changes were considered secondary to dilation of the endolymphatic space. True signs of degeneration, seldom seen in these specimens were intraepithelial cysts, necrotic cells, and dark homogeneous masses in the sensory epithelium and dilated nerve fibers in the subepithelial tissue. No general degeneration of the utricular macula or ampullar crista seems to occur in Meniere's disease.", "contents": "Vestibular sensory epithelium in Meniere's disease. Utricle and horizontal semicircular canal ampulla removed during labyrinthectomy from 11 patients with advanced Meniere's disease were studied under light and electron microscope. In light microscopy the epithelium of both sensory areas appeared fairly normal. Many sensory cells revealed fluid-filled crescents at the nucleus and many cells were extensively vacuolated. Ultrastructurally, a part of the observations were apparently artifacts due to specimen handling; some were normal, age-related findings. Some changes were considered secondary to dilation of the endolymphatic space. True signs of degeneration, seldom seen in these specimens were intraepithelial cysts, necrotic cells, and dark homogeneous masses in the sensory epithelium and dilated nerve fibers in the subepithelial tissue. No general degeneration of the utricular macula or ampullar crista seems to occur in Meniere's disease."}
{"id": "PMID:464889", "title": "Ophthalmoplethysmography in head and neck surgery.", "content": "Ophthalmoplethysmography (OPG) is a simple, noninvasive diagnostic test in which the cerebral hemispheric blood flow can be indirectly measured. Its use in head and neck surgery is invaluable because it enables the surgeon to predict preoperatively whether a patient will survive carotid artery resection. In a three-year period, nine patients have survived carotid resection on the basis of favorable OPGs, without a death or permanent neurologic complication. A comparison of the results of OPG testing with intraoperative measurement of internal carotid artery pressure showed close correlation in 14 of 20 patients who were tested. Lack of correlation in four patients can be explained by the circumstances of the intraoperative test. This study's findings demonstrate that this test has high clinical reliability.", "contents": "Ophthalmoplethysmography in head and neck surgery. Ophthalmoplethysmography (OPG) is a simple, noninvasive diagnostic test in which the cerebral hemispheric blood flow can be indirectly measured. Its use in head and neck surgery is invaluable because it enables the surgeon to predict preoperatively whether a patient will survive carotid artery resection. In a three-year period, nine patients have survived carotid resection on the basis of favorable OPGs, without a death or permanent neurologic complication. A comparison of the results of OPG testing with intraoperative measurement of internal carotid artery pressure showed close correlation in 14 of 20 patients who were tested. Lack of correlation in four patients can be explained by the circumstances of the intraoperative test. This study's findings demonstrate that this test has high clinical reliability."}
{"id": "PMID:464897", "title": "Home or hospital?", "content": "That life begins at home is the desire of most women, and the fear of most obstetricians. The following article attempts  to look at some facets of the problem.", "contents": "Home or hospital? That life begins at home is the desire of most women, and the fear of most obstetricians. The following article attempts  to look at some facets of the problem."}
{"id": "PMID:464892", "title": "Study of the hearing losses of industrial workers with occupational noise exposure, using statistical methods for the analysis of qualitative data.", "content": "The application of a polychotomous generalization of the linear probabilistic model of Cox is described, which allows the investigation of the effects of different noise levels with various durations of exposure on hearing. If the model assumptions are not rejected, it is possible to separate the effect of noise exposure from the effect of age. The results will thus show to what extent the hearing losses of an industrial worker are due to presbyacusis or to noise exposure. An empirical investigation was based on the evaluation of hearing losses at the audiometric frequencies 1, 2 and 3 kHz in 35 212 industrial workers of both sexes. It was attempted to predict the distribution of hearing losses in the left and the right ear as an additive function of the effect of noise exposure, age and sex. The parameters describing the effect of age increased linearly. Noise levels equal to and above 85 dB(A) have a noxious influence on hearing. Furthermore, hearing loss does not increase continuously with duration of exposure; from 15 years onwards, no essential increase in hearing loss appears which can be attributed to noise at work.", "contents": "Study of the hearing losses of industrial workers with occupational noise exposure, using statistical methods for the analysis of qualitative data. The application of a polychotomous generalization of the linear probabilistic model of Cox is described, which allows the investigation of the effects of different noise levels with various durations of exposure on hearing. If the model assumptions are not rejected, it is possible to separate the effect of noise exposure from the effect of age. The results will thus show to what extent the hearing losses of an industrial worker are due to presbyacusis or to noise exposure. An empirical investigation was based on the evaluation of hearing losses at the audiometric frequencies 1, 2 and 3 kHz in 35 212 industrial workers of both sexes. It was attempted to predict the distribution of hearing losses in the left and the right ear as an additive function of the effect of noise exposure, age and sex. The parameters describing the effect of age increased linearly. Noise levels equal to and above 85 dB(A) have a noxious influence on hearing. Furthermore, hearing loss does not increase continuously with duration of exposure; from 15 years onwards, no essential increase in hearing loss appears which can be attributed to noise at work."}
{"id": "PMID:464898", "title": "Induction and enhancement of labour.", "content": "The present trend to \"natural childbirth\" has prompted a critical review of induction of labour, with special reference to physiological and pharmacological aspects as well as indications, contraindications, complications and techniques for induction of labour. Enhancement of labour is also discussed.", "contents": "Induction and enhancement of labour. The present trend to \"natural childbirth\" has prompted a critical review of induction of labour, with special reference to physiological and pharmacological aspects as well as indications, contraindications, complications and techniques for induction of labour. Enhancement of labour is also discussed."}
{"id": "PMID:464899", "title": "Prolonged labour.", "content": "Although prolonged labour may be a decreasing obstetrical problem, the importance of its implications should not be overlooked. This article discusses the causes of arrested labour, its complications and their management.", "contents": "Prolonged labour. Although prolonged labour may be a decreasing obstetrical problem, the importance of its implications should not be overlooked. This article discusses the causes of arrested labour, its complications and their management."}
{"id": "PMID:464893", "title": "Critical bandwidth in Meni\u00e8re's disease.", "content": "The critical bandwidth in loudness summation was estimated in 20 patients with typical Meni\u00e8re's disease using noise bands centered around 1 kHz. A reduction of the normal loudness difference between broad-band noise and narrow-band noise was present at all except the highest levels. Judged individually, 7 of the 20 patients appeared to have a widened critical band, but in the pooled data the size of the critical band was normal. This was the case in patients with a hearing loss less than 50 dB HL as well as in patients with a hearing loss greater than or equal to 50 dB HL. Expressed in terms of the critical band mechanism as an internal filter system, the single filter appears to have normal bandwidth but the interaction between adjacent filters is defective. The anatomical localisation of this interaction is discussed.", "contents": "Critical bandwidth in Meni\u00e8re's disease. The critical bandwidth in loudness summation was estimated in 20 patients with typical Meni\u00e8re's disease using noise bands centered around 1 kHz. A reduction of the normal loudness difference between broad-band noise and narrow-band noise was present at all except the highest levels. Judged individually, 7 of the 20 patients appeared to have a widened critical band, but in the pooled data the size of the critical band was normal. This was the case in patients with a hearing loss less than 50 dB HL as well as in patients with a hearing loss greater than or equal to 50 dB HL. Expressed in terms of the critical band mechanism as an internal filter system, the single filter appears to have normal bandwidth but the interaction between adjacent filters is defective. The anatomical localisation of this interaction is discussed."}
{"id": "PMID:464900", "title": "The Leboyer method.", "content": "The method described is the reception of the newborn on the assumption that the baby is a sensitive, feeling being. The reasons for Leboyer's concept are discussed and the benefits to the parent-child bond are emphasized.", "contents": "The Leboyer method. The method described is the reception of the newborn on the assumption that the baby is a sensitive, feeling being. The reasons for Leboyer's concept are discussed and the benefits to the parent-child bond are emphasized."}
{"id": "PMID:464894", "title": "Consonant discrimination as a function of presentation level.", "content": "This study examined the effect of presentation level (seven levels ranging from 0 to 60 dB re the subject's SRT) on the discrimination of 20 consonants spoken in CV syllables. The subjects were 10 young adults with normal hearing. It was determined that the performance-intensity functions for individual consonants varied widely. Some sounds were not identified correctly 100% of the time even at the highest level tested, whereas other sounds were consistently identified at 10 dB SL. The data were considered with respect to stimulus and response bias and also were subjected to a feature analysis using features of voice, manner and place.", "contents": "Consonant discrimination as a function of presentation level. This study examined the effect of presentation level (seven levels ranging from 0 to 60 dB re the subject's SRT) on the discrimination of 20 consonants spoken in CV syllables. The subjects were 10 young adults with normal hearing. It was determined that the performance-intensity functions for individual consonants varied widely. Some sounds were not identified correctly 100% of the time even at the highest level tested, whereas other sounds were consistently identified at 10 dB SL. The data were considered with respect to stimulus and response bias and also were subjected to a feature analysis using features of voice, manner and place."}
{"id": "PMID:464901", "title": "The technique of episiotomy.", "content": "Episiotomy is frequently performed, with an incidence of 70-80 per cent in someunits, but is still often poorly executed and repaired with  little thought as to the importance of a supple pain-free scar.", "contents": "The technique of episiotomy. Episiotomy is frequently performed, with an incidence of 70-80 per cent in someunits, but is still often poorly executed and repaired with  little thought as to the importance of a supple pain-free scar."}
{"id": "PMID:464895", "title": "Effects of room reverberation upon hearing aid quality judgments.", "content": "Hearing-aid-processed speech recorded in reverberant and nonreverberant environments was ranked utilizing quality judgment and speech discrimination tasks. 21 normally hearing and 20 hearing-impaired listeners ranked the hearing-aid-processed speech. Quality judgments in reverberant environments may yield important information with respect to hearing aid selection. In contrast, speech discrimination rankings in a nonreverberant environment appeared to be similar to the speech discrimination rankings obtained in the reverberant condition.", "contents": "Effects of room reverberation upon hearing aid quality judgments. Hearing-aid-processed speech recorded in reverberant and nonreverberant environments was ranked utilizing quality judgment and speech discrimination tasks. 21 normally hearing and 20 hearing-impaired listeners ranked the hearing-aid-processed speech. Quality judgments in reverberant environments may yield important information with respect to hearing aid selection. In contrast, speech discrimination rankings in a nonreverberant environment appeared to be similar to the speech discrimination rankings obtained in the reverberant condition."}
{"id": "PMID:464903", "title": "Adolescent pregnancy in Australia. A hazard of early sexual intercourse.", "content": "The incidence in Australia of pregnancy in women under the age of twenty years is increasing, and constitutes a major medical and social problem. The outcome of these pregnancies has changed greatly during the past decade: more unmarried teenaged mothers are keeping their babies, fewer young women are marrying because of pregnancy, and the number of adolescents undergoing induced abortion continues to rise. Some suggestions for the prevention of adolescent pregnancy are discussed in this paper.", "contents": "Adolescent pregnancy in Australia. A hazard of early sexual intercourse. The incidence in Australia of pregnancy in women under the age of twenty years is increasing, and constitutes a major medical and social problem. The outcome of these pregnancies has changed greatly during the past decade: more unmarried teenaged mothers are keeping their babies, fewer young women are marrying because of pregnancy, and the number of adolescents undergoing induced abortion continues to rise. Some suggestions for the prevention of adolescent pregnancy are discussed in this paper."}
{"id": "PMID:464908", "title": "Attitides towards peer review.", "content": "The Australian Commonwealth Minister for Health called on the Australian Medical standards in Australia. Among the initial responses of the AMA was the organization of a National Seminar of Peer Review. Participants were asked to identify questions they would like to have answered about peer review and about aspects of a peer review system which they would perceive as necessary for its acceptability. Responses to these questions are analyzed and discussed. Implications for the introduction of peer review in Australia are examined, and strategies for gaining the commitment of the profession to the implementation of peer review are suggested.", "contents": "Attitides towards peer review. The Australian Commonwealth Minister for Health called on the Australian Medical standards in Australia. Among the initial responses of the AMA was the organization of a National Seminar of Peer Review. Participants were asked to identify questions they would like to have answered about peer review and about aspects of a peer review system which they would perceive as necessary for its acceptability. Responses to these questions are analyzed and discussed. Implications for the introduction of peer review in Australia are examined, and strategies for gaining the commitment of the profession to the implementation of peer review are suggested."}
{"id": "PMID:464909", "title": "General practitioners in the Sydney metropolitan health regions.", "content": "In a comprehensive survey of the number of general practitioners in the four metropolitan health regions of Sydney, 2,328 general practitioners were identified--the equivalent of 2,225 full time practitioners. This resulted in a ratio of one general practitioner to every 1,393 of the Sydney population.", "contents": "General practitioners in the Sydney metropolitan health regions. In a comprehensive survey of the number of general practitioners in the four metropolitan health regions of Sydney, 2,328 general practitioners were identified--the equivalent of 2,225 full time practitioners. This resulted in a ratio of one general practitioner to every 1,393 of the Sydney population."}
{"id": "PMID:464913", "title": "Amniocentesis in the early second trimester of pregnancy and maternal anxiety.", "content": "Anxiety state and trait were measured before and after amniocentesis early in the second trimester of pregnancy, in a group of 28 women who were at risk of bearing an abnormal foetus. A significant decrease in maternal anxiety occurred in the majority of women after receiving the results of the amniocentesis. It is concluded that amniocentesis for intrauterine diagnosis of foetal abnormalities makes a significant contribution to the psychological wellbeing of those pregnant women who are at greater risk of having an abnormal baby.", "contents": "Amniocentesis in the early second trimester of pregnancy and maternal anxiety. Anxiety state and trait were measured before and after amniocentesis early in the second trimester of pregnancy, in a group of 28 women who were at risk of bearing an abnormal foetus. A significant decrease in maternal anxiety occurred in the majority of women after receiving the results of the amniocentesis. It is concluded that amniocentesis for intrauterine diagnosis of foetal abnormalities makes a significant contribution to the psychological wellbeing of those pregnant women who are at greater risk of having an abnormal baby."}
{"id": "PMID:464914", "title": "Mammography--use or abuse.", "content": "Excluding the question of cost, at the present time the benefit of mammography associated with physical examination over the age of 50 appears proven as an intervention measure. With recent advances in radiological techniques, women under 50 appear to be advantaged by the performance of mammography but the benefit through the use of screening has yet to be proven by clinical trial. Screening might be restricted to the high risk group of patients with previous or present breast disorders and a family history of breast cancer in close relatives. Promotion of BSE and screening needs continuing education of the public, particularly concerning the value of early detection to successful treatment, and requires agreement on the methods and indications for screening (and subsequent treatment) by those advocating the programmes in order to keep the public's confidence. Overenthusiasm and polarity of views are to be avoided, so that health educators may be allowed to give the same message for credibility's sake.", "contents": "Mammography--use or abuse. Excluding the question of cost, at the present time the benefit of mammography associated with physical examination over the age of 50 appears proven as an intervention measure. With recent advances in radiological techniques, women under 50 appear to be advantaged by the performance of mammography but the benefit through the use of screening has yet to be proven by clinical trial. Screening might be restricted to the high risk group of patients with previous or present breast disorders and a family history of breast cancer in close relatives. Promotion of BSE and screening needs continuing education of the public, particularly concerning the value of early detection to successful treatment, and requires agreement on the methods and indications for screening (and subsequent treatment) by those advocating the programmes in order to keep the public's confidence. Overenthusiasm and polarity of views are to be avoided, so that health educators may be allowed to give the same message for credibility's sake."}
{"id": "PMID:464916", "title": "Breast reconstruction after mastectomy.", "content": "Breast reconstruction is being performed with increased frequency, encouraged largely by the positive reaction of satisfied patients, and enabled by more recent advances in plastic surgery. Many of the disadvantages of external prosthesis (discomfort, insecurity, lack of permanence, absent cleavage) are overcome by surgical reconstruction, allowing the patient's greater confidence and more normal life style.", "contents": "Breast reconstruction after mastectomy. Breast reconstruction is being performed with increased frequency, encouraged largely by the positive reaction of satisfied patients, and enabled by more recent advances in plastic surgery. Many of the disadvantages of external prosthesis (discomfort, insecurity, lack of permanence, absent cleavage) are overcome by surgical reconstruction, allowing the patient's greater confidence and more normal life style."}
{"id": "PMID:464935", "title": "Anthelmintic efficiency of oxfendazole, fenbendazole and levamisole against naturally acquired infections of Ostertagia ostertagi and Trichostrongylus axei in cattle.", "content": "The anthelmintic efficiencies of oxfendazole, fenbendazole and levamisole, each at 4 dose rates spanning the manufacturers' recommended dosages were compared in beef cattle with naturally acquired infections of Ostertagia ostertagi and Trichostrongylus axei. In 8 of the 9 cases tested there was no significant increase in anthelmintic efficiency due to increased dose rates of any of the drugs. Percentage efficiencies and their standard errors, calculated from mean worm counts of pooled data for adult worms, developing 4th stage and early 4th stage larvae of O. ostertagi, were respectively, 86.9+/-4.2, 77.4+/-6.1, 74.5+/-7.3 for oxfendazole, 93.7+/-2.0. 80.7+/-6.8, 59.6+/-13.9 for fenbendazole and 69.7+/-6.9, 39.4+/-14.8, 31.2+/-22.6 for levamisole. Counts of O. ostertagi from cattle treated with oxfendazole and fenbendazole were not significantly different, but both were significantly lower than those from cattle given levamisole. Efficiency against T. axei exceeded 99% for all drugs. Practical implications for therapy and preventative control of ostertagiasis are discussed.", "contents": "Anthelmintic efficiency of oxfendazole, fenbendazole and levamisole against naturally acquired infections of Ostertagia ostertagi and Trichostrongylus axei in cattle. The anthelmintic efficiencies of oxfendazole, fenbendazole and levamisole, each at 4 dose rates spanning the manufacturers' recommended dosages were compared in beef cattle with naturally acquired infections of Ostertagia ostertagi and Trichostrongylus axei. In 8 of the 9 cases tested there was no significant increase in anthelmintic efficiency due to increased dose rates of any of the drugs. Percentage efficiencies and their standard errors, calculated from mean worm counts of pooled data for adult worms, developing 4th stage and early 4th stage larvae of O. ostertagi, were respectively, 86.9+/-4.2, 77.4+/-6.1, 74.5+/-7.3 for oxfendazole, 93.7+/-2.0. 80.7+/-6.8, 59.6+/-13.9 for fenbendazole and 69.7+/-6.9, 39.4+/-14.8, 31.2+/-22.6 for levamisole. Counts of O. ostertagi from cattle treated with oxfendazole and fenbendazole were not significantly different, but both were significantly lower than those from cattle given levamisole. Efficiency against T. axei exceeded 99% for all drugs. Practical implications for therapy and preventative control of ostertagiasis are discussed."}
{"id": "PMID:464936", "title": "Thiabendazole resistance in field populations of Haemonchus contortus.", "content": "Six populations of H. contortus were selected for a study of thiabendazole resistance from a collection of 40 populations made during a survey of the efficiency of thiabendazole on the Northern Tablelands of New South Wales. Based on survey results, 3 of these populations were considered susceptible and the remaining 3 were considered resistant. However, when these populations were compared with a known susceptible strain on the ability of their eggs to hatch in a solution containing thiabendazole and 0.1% NaCl all 6 had significantly greater LC50's. The resistance ratios of the LC50 for each of the 6 populations to that of the known susceptible strain were 5.1, 4.3, 3.2, 3.1, 2.3, and 1.6 respectively. Following dosing of their host with 44 mg/kg thiabendazole the resistance ratios of the survivors increased to 5.4, 5.1, 4.7, 4.4, 3.1 and 2.4. Eggs produced by the F1 generation of the worms surviving 44 mg/kg thiabendazole did not revert back to the lower  LC50's of the unselected parents. Rather the LC50 remained at a level near that of a known highly resistant strain of H. contortus.", "contents": "Thiabendazole resistance in field populations of Haemonchus contortus. Six populations of H. contortus were selected for a study of thiabendazole resistance from a collection of 40 populations made during a survey of the efficiency of thiabendazole on the Northern Tablelands of New South Wales. Based on survey results, 3 of these populations were considered susceptible and the remaining 3 were considered resistant. However, when these populations were compared with a known susceptible strain on the ability of their eggs to hatch in a solution containing thiabendazole and 0.1% NaCl all 6 had significantly greater LC50's. The resistance ratios of the LC50 for each of the 6 populations to that of the known susceptible strain were 5.1, 4.3, 3.2, 3.1, 2.3, and 1.6 respectively. Following dosing of their host with 44 mg/kg thiabendazole the resistance ratios of the survivors increased to 5.4, 5.1, 4.7, 4.4, 3.1 and 2.4. Eggs produced by the F1 generation of the worms surviving 44 mg/kg thiabendazole did not revert back to the lower  LC50's of the unselected parents. Rather the LC50 remained at a level near that of a known highly resistant strain of H. contortus."}
{"id": "PMID:464937", "title": "Effect of birth weight on efficiency of beef production.", "content": "The large 'lean beef' breeds are vulnerable to calving difficulties and losses. Dystocia is related to high birth weight. Data recorded from evaluation experiments show that the birth weight of the 'lean beef' taurus crossbreeds was high (39+/-2.1 kg). Indicus beef genotypes were selected with the birth weight (29+/-1.9 kg) significantly lower (P is less than 0.01) without a depression in the final yield of beef. To increase efficiency of beef production the lower birth weight factor needs be incorporated into genotypes for high viability and growth. Profitability depends on output per dam, in relation to inputs, on a herd basis. Hence, progress may depend on the application of such changes of genotype.", "contents": "Effect of birth weight on efficiency of beef production. The large 'lean beef' breeds are vulnerable to calving difficulties and losses. Dystocia is related to high birth weight. Data recorded from evaluation experiments show that the birth weight of the 'lean beef' taurus crossbreeds was high (39+/-2.1 kg). Indicus beef genotypes were selected with the birth weight (29+/-1.9 kg) significantly lower (P is less than 0.01) without a depression in the final yield of beef. To increase efficiency of beef production the lower birth weight factor needs be incorporated into genotypes for high viability and growth. Profitability depends on output per dam, in relation to inputs, on a herd basis. Hence, progress may depend on the application of such changes of genotype."}
{"id": "PMID:464938", "title": "Toxicity of Crotalaria Goreensis for chickens.", "content": "Ground Crotalaria goreensis seed fed to day-old cockerel chickens at 3 different rates in a commercial ration of chick starter mash depressed growth rates. Moist faeces were common. Lesions consistently found included ulceration of the proximal large intestine and a dark grey discolouration of the glandular portion of the proventriculus. C. goreensis should be excluded from all poultry rations.", "contents": "Toxicity of Crotalaria Goreensis for chickens. Ground Crotalaria goreensis seed fed to day-old cockerel chickens at 3 different rates in a commercial ration of chick starter mash depressed growth rates. Moist faeces were common. Lesions consistently found included ulceration of the proximal large intestine and a dark grey discolouration of the glandular portion of the proventriculus. C. goreensis should be excluded from all poultry rations."}
{"id": "PMID:464939", "title": "Renal failure, hyperparathyroidism and hypercalcaemia in a dog.", "content": "A 4-year Basset bitch with a 9-week history of depression, lethargy, inappetence and weight loss was found to have azotaemia, hypercalcaemia and hyperphosphataemia. Laparotomy and kidney biopsy revealed end-stage renal disease and the dog was killed. Hyperplasia of all 4 parathyroid glands was found at autopsy. The presumptive diagnosis was idiopathic renal failure with resulting tertiary hyperparathyroidism.", "contents": "Renal failure, hyperparathyroidism and hypercalcaemia in a dog. A 4-year Basset bitch with a 9-week history of depression, lethargy, inappetence and weight loss was found to have azotaemia, hypercalcaemia and hyperphosphataemia. Laparotomy and kidney biopsy revealed end-stage renal disease and the dog was killed. Hyperplasia of all 4 parathyroid glands was found at autopsy. The presumptive diagnosis was idiopathic renal failure with resulting tertiary hyperparathyroidism."}
{"id": "PMID:464940", "title": "Renal cortical hypoplasia in a dog.", "content": "A case of renal cortical hypoplasia in a Cocker Spaniel bitch is presented. The dog, under clinical observation between the ages of 15 to 26 months, was found to have advancing chronic renal insufficiency. Necropsy examination revealed a markedly hypoplastic renal cortex with a reduced number of glomeruli, some dilated Bowman's capsules, small glomerular tufts, and early interstitial nephritis and fibrosis characteristic of renal cortical hypoplasia.", "contents": "Renal cortical hypoplasia in a dog. A case of renal cortical hypoplasia in a Cocker Spaniel bitch is presented. The dog, under clinical observation between the ages of 15 to 26 months, was found to have advancing chronic renal insufficiency. Necropsy examination revealed a markedly hypoplastic renal cortex with a reduced number of glomeruli, some dilated Bowman's capsules, small glomerular tufts, and early interstitial nephritis and fibrosis characteristic of renal cortical hypoplasia."}
{"id": "PMID:464941", "title": "Ascending urinary tract infection in ponies.", "content": "Two young ponies had the prescrotal portion of their penises accidentally amputated during castration. They both developed ascending urinary tract infections over the next 4 weeks. One pony had a necrotising cystitis and pyelonephritis, the other improved markedly after a urethrostomy had been performed but was destroyed several months later.", "contents": "Ascending urinary tract infection in ponies. Two young ponies had the prescrotal portion of their penises accidentally amputated during castration. They both developed ascending urinary tract infections over the next 4 weeks. One pony had a necrotising cystitis and pyelonephritis, the other improved markedly after a urethrostomy had been performed but was destroyed several months later."}
{"id": "PMID:464951", "title": "Dynamic factors influencing the production of lung injury in rabbits subjected to blunt chest wall impact.", "content": "Anesthetized rabbits were exposed to blunt impact on the right chest wall. The controlled impact was delivered with a special machine equipped with a captive piston driven by a freely falling weight. Chest wall deflections were recorded in order to study the effects of magnitude and rate of deformation of the thorax on the resulting lung injury. The ranges of magnitude and rate of deformation corresponded to an inward deflection of the chest wall of about 5-60% of the lateral diameter of the chest, and to a velocity of the chest wall of about 2-20 m/s. Impulses delivered to the chest and intrathoracic pressures were also studied. Severe hemorrhages in the lungs were produced at velocities of the chest wall exceeding about 10 m/s. For velocities of the chest wall below about 5 m/s, lethal injuries were produced without severe hemorrhages in the lungs.", "contents": "Dynamic factors influencing the production of lung injury in rabbits subjected to blunt chest wall impact. Anesthetized rabbits were exposed to blunt impact on the right chest wall. The controlled impact was delivered with a special machine equipped with a captive piston driven by a freely falling weight. Chest wall deflections were recorded in order to study the effects of magnitude and rate of deformation of the thorax on the resulting lung injury. The ranges of magnitude and rate of deformation corresponded to an inward deflection of the chest wall of about 5-60% of the lateral diameter of the chest, and to a velocity of the chest wall of about 2-20 m/s. Impulses delivered to the chest and intrathoracic pressures were also studied. Severe hemorrhages in the lungs were produced at velocities of the chest wall exceeding about 10 m/s. For velocities of the chest wall below about 5 m/s, lethal injuries were produced without severe hemorrhages in the lungs."}
{"id": "PMID:464952", "title": "Biodynamic effects of canopy loss in the TF-15 aircraft.", "content": "In-flight canopy loss in high-speed fighter or trainer aircraft poses serious aeromedical problems for the crew-members. To assess these problems in the TF-15, a series of canopy-off tests were conducted. Preliminary phases of the test consisted of a taxi and a flight test up to 485 knots with pilot in the front seat and an instrumented dummy in the back seat. Upon evaluation of these results, a taxi phase and a flight phase were conducted with a pilot in the front seat and a live subject in the back seat. Maximum air-speed attained was 410 knots. The front crewmember experienced no serious control or medical problems during all phases. The back crewmember did experience problems such as loss of communications, loss of vision, helmet lift, high frequency head buffet, and exhalation difficulties. Airspeed, seat height, and body position had the greatest effect on the severity of the windblast. Angle of bank, angle of attack, and acceleration had little effect. The aft crewmember was able to significantly decrease windblast effects by leaning forward and pulling his head and helmet forward and down with his hand.", "contents": "Biodynamic effects of canopy loss in the TF-15 aircraft. In-flight canopy loss in high-speed fighter or trainer aircraft poses serious aeromedical problems for the crew-members. To assess these problems in the TF-15, a series of canopy-off tests were conducted. Preliminary phases of the test consisted of a taxi and a flight test up to 485 knots with pilot in the front seat and an instrumented dummy in the back seat. Upon evaluation of these results, a taxi phase and a flight phase were conducted with a pilot in the front seat and a live subject in the back seat. Maximum air-speed attained was 410 knots. The front crewmember experienced no serious control or medical problems during all phases. The back crewmember did experience problems such as loss of communications, loss of vision, helmet lift, high frequency head buffet, and exhalation difficulties. Airspeed, seat height, and body position had the greatest effect on the severity of the windblast. Angle of bank, angle of attack, and acceleration had little effect. The aft crewmember was able to significantly decrease windblast effects by leaning forward and pulling his head and helmet forward and down with his hand."}
{"id": "PMID:464953", "title": "Plasma electrolytes in relation to altitude tolerance in rats.", "content": "Plasma concentration of Na+, K+ and Cl- ions did not change significantly whereas that of HCO3- dropped to one-third of its initial value in rats during their 15 min of gasping at a simulated altitude of 10,000 m at 33 degrees C, which was their survival threshold. Administration of methamphetamine (2 mg/kg), imipramine (2 mg/kg) and adrenaline (7 mg/kg) i.p. were ineffective in prolonging the survival time of rats, whereas acetazolamide (40 mg/kg), furosemide (2 mg/kg) and caffeine citrate (10 mg/kg) significantly increased their survival under hypoxia, (p less than 0.001, less than 0.01, and less than 0.05, respectively).", "contents": "Plasma electrolytes in relation to altitude tolerance in rats. Plasma concentration of Na+, K+ and Cl- ions did not change significantly whereas that of HCO3- dropped to one-third of its initial value in rats during their 15 min of gasping at a simulated altitude of 10,000 m at 33 degrees C, which was their survival threshold. Administration of methamphetamine (2 mg/kg), imipramine (2 mg/kg) and adrenaline (7 mg/kg) i.p. were ineffective in prolonging the survival time of rats, whereas acetazolamide (40 mg/kg), furosemide (2 mg/kg) and caffeine citrate (10 mg/kg) significantly increased their survival under hypoxia, (p less than 0.001, less than 0.01, and less than 0.05, respectively)."}
{"id": "PMID:464954", "title": "Changes in apparent body orientation and sensory localization induced by vibration of postural muscles: vibratory myesthetic illusions.", "content": "Illusions of continuous body tilt and rotation can be elicited by vibrating postural muscles of subjects standing in the dark. During such illusory motion, the apparent pivot point of the body can be influenced systematically by touch and pressure cues. Strong apparent movement is sometimes accompained by nystagmus of compensatory sign. If a small target light is visible during vibration, visual motion of like direction and velocity will accompany the illusory body motion. We have designated this pattern of apparent body and visual motion the \"propriogyral illusion\". Full room illumination abolishes both components of the propiogyral illusion. When the propriogyral illusion is being experienced, there is a dissociation between apparent displacement and apparent velocity; the extent of displacement is always less than would be expected on the basis of apparent velocity. The illusions of continuous body motion and the propriogyral illusion represent elements of a general set of vibratory myesthetic illusions that influence apparent posture, sensory, localization, and position sense of the body. These illusions demonstrate an important contribution of muscle afferent and touch-pressure information to the central mechanisms that determine apparent spatial orientation and visual localization. They also provide evidence that somatosensory information about orientation can influence oculomotor control.", "contents": "Changes in apparent body orientation and sensory localization induced by vibration of postural muscles: vibratory myesthetic illusions. Illusions of continuous body tilt and rotation can be elicited by vibrating postural muscles of subjects standing in the dark. During such illusory motion, the apparent pivot point of the body can be influenced systematically by touch and pressure cues. Strong apparent movement is sometimes accompained by nystagmus of compensatory sign. If a small target light is visible during vibration, visual motion of like direction and velocity will accompany the illusory body motion. We have designated this pattern of apparent body and visual motion the \"propriogyral illusion\". Full room illumination abolishes both components of the propiogyral illusion. When the propriogyral illusion is being experienced, there is a dissociation between apparent displacement and apparent velocity; the extent of displacement is always less than would be expected on the basis of apparent velocity. The illusions of continuous body motion and the propriogyral illusion represent elements of a general set of vibratory myesthetic illusions that influence apparent posture, sensory, localization, and position sense of the body. These illusions demonstrate an important contribution of muscle afferent and touch-pressure information to the central mechanisms that determine apparent spatial orientation and visual localization. They also provide evidence that somatosensory information about orientation can influence oculomotor control."}
{"id": "PMID:464955", "title": "Effects of phase manipulation on speech intelligibility through communication headsets.", "content": "A masking level difference (MLD) paradigm was established by rewiring the earphones of a communications headset out-of-phase. Essentially no release from masking could be measured, however, on a crew of listeners in the cabin of light aircraft. The experiment was replicated in the laboratory so that the exact phase of the aircraft noise masker could be controlled. The substantial MLD obtained in this environment led to the conclusion that the noise reaching the ear in the cockpit was of random phase, which almost eliminates the MLD. Therefore, rewiring headsets out-of-phase provides no advantage in intelligibility.", "contents": "Effects of phase manipulation on speech intelligibility through communication headsets. A masking level difference (MLD) paradigm was established by rewiring the earphones of a communications headset out-of-phase. Essentially no release from masking could be measured, however, on a crew of listeners in the cabin of light aircraft. The experiment was replicated in the laboratory so that the exact phase of the aircraft noise masker could be controlled. The substantial MLD obtained in this environment led to the conclusion that the noise reaching the ear in the cockpit was of random phase, which almost eliminates the MLD. Therefore, rewiring headsets out-of-phase provides no advantage in intelligibility."}
{"id": "PMID:464956", "title": "Tissue gas and blood analyses of human subjects breathing 80% argon and 20% oxygen.", "content": "Eight human volunteers, individually studied in a hyperbaric chamber, breathed: 1) air at 1 ATA; 2) 80% argon and 20% oxygen and 1 ATA for 30 min; 3) air at 1 ATA for 30 min; 4) 100% 02 at 1 ATA for 30 min; 5) air at 1 ATA for 30 min; 6) 100% O2 at 2 ATA for 60 min; and 7) 80% argon and 20% oxygen at 1 ATA for 30 min. Oxygen, carbon dioxide, nitrogen, and argon tensions were measured in muscle and subcutaneous tissue by mass spectroscopic analyses. Venous blood obtained at regular intervals was analyzed for coagulation and fibrinolytic factors. Inert gas narcosis was not observed. After breathing argon for 30 min, muscle argon tensions were almost three times subcutaneous tensions. Argon wash-in mirrored nitrogen wash-out. Argon wash-in and wash-out had no effect on tissue Po2 or Pco2. Coagulation and fibrinolytic changes usually associated with vascular bubbles were absent.", "contents": "Tissue gas and blood analyses of human subjects breathing 80% argon and 20% oxygen. Eight human volunteers, individually studied in a hyperbaric chamber, breathed: 1) air at 1 ATA; 2) 80% argon and 20% oxygen and 1 ATA for 30 min; 3) air at 1 ATA for 30 min; 4) 100% 02 at 1 ATA for 30 min; 5) air at 1 ATA for 30 min; 6) 100% O2 at 2 ATA for 60 min; and 7) 80% argon and 20% oxygen at 1 ATA for 30 min. Oxygen, carbon dioxide, nitrogen, and argon tensions were measured in muscle and subcutaneous tissue by mass spectroscopic analyses. Venous blood obtained at regular intervals was analyzed for coagulation and fibrinolytic factors. Inert gas narcosis was not observed. After breathing argon for 30 min, muscle argon tensions were almost three times subcutaneous tensions. Argon wash-in mirrored nitrogen wash-out. Argon wash-in and wash-out had no effect on tissue Po2 or Pco2. Coagulation and fibrinolytic changes usually associated with vascular bubbles were absent."}
{"id": "PMID:464957", "title": "Mechanical vs. ischemic mechanisms for decompression sickness.", "content": "We used 20 kangaroo rats to investigate the effect of exposure to low oxygen levels (0.11 Atm 02 inspired partial pressure) prior to decompression from a steady-state condition. This hypoxia was found to afford significant protection against limb bends as simulated in those animals by tail biting. Yet, it potentiated neurologic symptoms compared with a control exposure on air with the same level of nitrogen supersaturation. However the incidence of simulated limb bends in the same animals was the same with hypoxia as with another control exposure at a pressure estimated to give extravascular bubbles of the same size upon decompression. The results are, therefore, consistent with a simple mechanical basis for limb bends, but are difficult to explain by any ischemic mechanism since a general hypoxia exacerbates any pain produced by oxygen deficiency in the tissues. However, the reverse may be true for some forms of neurologic decompression sickness and the two such cases reported here are consistent with that view, although not statistically significant.", "contents": "Mechanical vs. ischemic mechanisms for decompression sickness. We used 20 kangaroo rats to investigate the effect of exposure to low oxygen levels (0.11 Atm 02 inspired partial pressure) prior to decompression from a steady-state condition. This hypoxia was found to afford significant protection against limb bends as simulated in those animals by tail biting. Yet, it potentiated neurologic symptoms compared with a control exposure on air with the same level of nitrogen supersaturation. However the incidence of simulated limb bends in the same animals was the same with hypoxia as with another control exposure at a pressure estimated to give extravascular bubbles of the same size upon decompression. The results are, therefore, consistent with a simple mechanical basis for limb bends, but are difficult to explain by any ischemic mechanism since a general hypoxia exacerbates any pain produced by oxygen deficiency in the tissues. However, the reverse may be true for some forms of neurologic decompression sickness and the two such cases reported here are consistent with that view, although not statistically significant."}
{"id": "PMID:464958", "title": "Responses of high altitude natives a standard cold test at sea level.", "content": "Studies have been conducted to evaluate the thermoregulatory, calorigenic, and peripheral vascular responses to cold of high altitude (HA) natives on their descent to sea level. Two groups of subjects (18 each), one representing high altitude (3500 m) natives and the other lowlanders, were exposed to a standard cold test at 10 degrees C wearing only shorts for 2 h. Their heart rate (HR), blood pressure (BP), oxygen consumption (VO2), oral temperature (Tor), mean weighted skin temperature (Ts) and shivering activity were recorded initially in a thermoneutral room, and 30 min intervals during the cold exposure. Afterwards, their cold-induced vasodilatation (CIVD) response was studied and the peripheral blood flow during local cold stress was calculated. The responses were statistically compared. The highlanders maintained significantly higher Tor, Tb (mean body temperature), Ts and peripheral Ts during cold stress, and they shivered much less and showed higher CIVD response and peripheral blood flow than the other group. The rise in VO2 on cold stress was identical. The observations showed better cold tolerance for high altitude natives compared to lowlanders, even at sea level.", "contents": "Responses of high altitude natives a standard cold test at sea level. Studies have been conducted to evaluate the thermoregulatory, calorigenic, and peripheral vascular responses to cold of high altitude (HA) natives on their descent to sea level. Two groups of subjects (18 each), one representing high altitude (3500 m) natives and the other lowlanders, were exposed to a standard cold test at 10 degrees C wearing only shorts for 2 h. Their heart rate (HR), blood pressure (BP), oxygen consumption (VO2), oral temperature (Tor), mean weighted skin temperature (Ts) and shivering activity were recorded initially in a thermoneutral room, and 30 min intervals during the cold exposure. Afterwards, their cold-induced vasodilatation (CIVD) response was studied and the peripheral blood flow during local cold stress was calculated. The responses were statistically compared. The highlanders maintained significantly higher Tor, Tb (mean body temperature), Ts and peripheral Ts during cold stress, and they shivered much less and showed higher CIVD response and peripheral blood flow than the other group. The rise in VO2 on cold stress was identical. The observations showed better cold tolerance for high altitude natives compared to lowlanders, even at sea level."}
{"id": "PMID:464959", "title": "Measurement and scaling of workload in complex performance.", "content": "Two groups of paid male volunteers (Groups I, N = 51, tested with identical schedules on two successive days; Group II, N = 43, tested on one day only) performed over nine intervals on various combinations of the six tasks of the CAMI Multiple Task Performance Battery. Two of the tasks involved monitoring static (lights) and dynamic (meters) processes, and four more-active tasks involved mental arithmetic, elementary problem solving, pattern identification, two-dimensional compensatory tracking. Five of the nine intervals provided different complex tasks consisting of concurrent monitoring tasks and two of the active tasks. Other trials provided data on the single active tasks as well as the combined monitoring tasks. The results indicated that all performance measures--a total of 12 for the six tasks--varied significantly as a function of the different task-combination conditions. A standard psychological scaling technique (Thurstone Case V) was applied to the monitoring data (response times for green and red lights, and for meter monitoring) to develop an index of workload for the five complex task combinations. Since better performance was presumed to indicate a lower workload, workload was inferred to increase as performance declined across conditions. The best performances, which were assigned scale values of 0, were found to be associated with single-task performances, as expected. Scale values for the complex task-combination conditions were found to be consistent between groups and between the two days of testing of Group I (r's of 0.947 to 0.993). Although the scale values are specific to the task and task-combination conditions employed, the scaling-procedure application shows promise for cases in which quantitative measures of performance can be acquired with moderately large samples of subjects (N's greater than 50).", "contents": "Measurement and scaling of workload in complex performance. Two groups of paid male volunteers (Groups I, N = 51, tested with identical schedules on two successive days; Group II, N = 43, tested on one day only) performed over nine intervals on various combinations of the six tasks of the CAMI Multiple Task Performance Battery. Two of the tasks involved monitoring static (lights) and dynamic (meters) processes, and four more-active tasks involved mental arithmetic, elementary problem solving, pattern identification, two-dimensional compensatory tracking. Five of the nine intervals provided different complex tasks consisting of concurrent monitoring tasks and two of the active tasks. Other trials provided data on the single active tasks as well as the combined monitoring tasks. The results indicated that all performance measures--a total of 12 for the six tasks--varied significantly as a function of the different task-combination conditions. A standard psychological scaling technique (Thurstone Case V) was applied to the monitoring data (response times for green and red lights, and for meter monitoring) to develop an index of workload for the five complex task combinations. Since better performance was presumed to indicate a lower workload, workload was inferred to increase as performance declined across conditions. The best performances, which were assigned scale values of 0, were found to be associated with single-task performances, as expected. Scale values for the complex task-combination conditions were found to be consistent between groups and between the two days of testing of Group I (r's of 0.947 to 0.993). Although the scale values are specific to the task and task-combination conditions employed, the scaling-procedure application shows promise for cases in which quantitative measures of performance can be acquired with moderately large samples of subjects (N's greater than 50)."}
{"id": "PMID:464960", "title": "Oculogravic illusion in response to straight-ahead acceleration of CF-104 aircraft.", "content": "Experimental subjects wore goggles that restricted monocular vision to a luminous line fixed relative to the head, and they were exposed on one occasion to a straight-ahead acceleration of an aircraft and on another occasion to a tilting chair. The magnitude of change of direction of the resultant accleration was the same on both occasions, but the perceived movement of the luminous line from the two stimuli was very different. In response to the aircraft stimulus, the oculogravic illusion was experienced and the luminous line was perceived as tilting relative to the subject, in response to the tilting chair stimulus, the line was perceived as remaining fixed relative to the subject. It was concluded that the oculogravic illusion, as experienced in the aircraft (and previously in centrifuges), is a true illusion and not merely a fact of physics.", "contents": "Oculogravic illusion in response to straight-ahead acceleration of CF-104 aircraft. Experimental subjects wore goggles that restricted monocular vision to a luminous line fixed relative to the head, and they were exposed on one occasion to a straight-ahead acceleration of an aircraft and on another occasion to a tilting chair. The magnitude of change of direction of the resultant accleration was the same on both occasions, but the perceived movement of the luminous line from the two stimuli was very different. In response to the aircraft stimulus, the oculogravic illusion was experienced and the luminous line was perceived as tilting relative to the subject, in response to the tilting chair stimulus, the line was perceived as remaining fixed relative to the subject. It was concluded that the oculogravic illusion, as experienced in the aircraft (and previously in centrifuges), is a true illusion and not merely a fact of physics."}
{"id": "PMID:464961", "title": "Cardiac response to whole-body heating.", "content": "Changes in ventricular function, as described by systolic time interval analysis, have been studied during heat stress and the time course of these changes, as well as changes in heart rate, have been investigated. Seven male and female subjects, 22-35 years of age, immersed their lower legs in water 42-44 degrees C for 30 min; their trunks and upper legs were enclosed in nonpermeable plastic to prevent evaporative heat loss. Total beat interval (R-R), left ventricular ejection time (LVET), pre-ejection period (PEP), arterial blood pressure, and oral temperature were monitored periodically during heating and recovery (post-hearing) periods. The stress induced significant (p less than 0.05) decreases in R-R and LVET by 5 min of heating which continued decreasing throughout the 30-min heating period. Heart rate accelerated from 72 up to 97 beats/min. LVET's during heat stress were shorter than those at similar resting heart rates. R-R and LVET were increased significantly by 1-min recovery but had not returned to preheating levels at 15 min. PEP, on the other hand, was not reduced until 15 min of heating, and did not start to recover until 3 min post-heating, after which it reached values exceeding control. A small increase in systolic blood pressure was maintained throughout heating and remained at 15 min recovery. Oral temperature increased approximately 1 degree C during the heating procedure; 50% of this was dissipated by 15 min recovery. Results are consistent with a two-stage cardiac response to heat-vagal withdrawal followed by a strong sympathetic outflow to the heart affecting both inotropic and chronotropic characteristics.", "contents": "Cardiac response to whole-body heating. Changes in ventricular function, as described by systolic time interval analysis, have been studied during heat stress and the time course of these changes, as well as changes in heart rate, have been investigated. Seven male and female subjects, 22-35 years of age, immersed their lower legs in water 42-44 degrees C for 30 min; their trunks and upper legs were enclosed in nonpermeable plastic to prevent evaporative heat loss. Total beat interval (R-R), left ventricular ejection time (LVET), pre-ejection period (PEP), arterial blood pressure, and oral temperature were monitored periodically during heating and recovery (post-hearing) periods. The stress induced significant (p less than 0.05) decreases in R-R and LVET by 5 min of heating which continued decreasing throughout the 30-min heating period. Heart rate accelerated from 72 up to 97 beats/min. LVET's during heat stress were shorter than those at similar resting heart rates. R-R and LVET were increased significantly by 1-min recovery but had not returned to preheating levels at 15 min. PEP, on the other hand, was not reduced until 15 min of heating, and did not start to recover until 3 min post-heating, after which it reached values exceeding control. A small increase in systolic blood pressure was maintained throughout heating and remained at 15 min recovery. Oral temperature increased approximately 1 degree C during the heating procedure; 50% of this was dissipated by 15 min recovery. Results are consistent with a two-stage cardiac response to heat-vagal withdrawal followed by a strong sympathetic outflow to the heart affecting both inotropic and chronotropic characteristics."}
{"id": "PMID:464962", "title": "Rotation at 30 RPM about the A axis after 6 hours in the 10 degree head-down position: effect on susceptibility to motion sickness.", "content": "Intraindividual differences in susceptibility to motion sickness were measured in 14 subjects for two conditions of rotation at 30 rpm in the 10 degree head-down position. In one condition, subjects were in the 10 degree head-down position for 6 h prior to the onset of rotation; in the other condition, the delay was only 15 min. In both conditions, there were changes in vital capacity, indicating a redistribution of movable body fluids. Subjects tended to be less susceptible to motion sickness when they were recumbent for 6 h prior to rotation. These results are countervidence for the hypothesis that shifts of body fluid are responsible in large part for the motion sickness elicited in orbital space flight.", "contents": "Rotation at 30 RPM about the A axis after 6 hours in the 10 degree head-down position: effect on susceptibility to motion sickness. Intraindividual differences in susceptibility to motion sickness were measured in 14 subjects for two conditions of rotation at 30 rpm in the 10 degree head-down position. In one condition, subjects were in the 10 degree head-down position for 6 h prior to the onset of rotation; in the other condition, the delay was only 15 min. In both conditions, there were changes in vital capacity, indicating a redistribution of movable body fluids. Subjects tended to be less susceptible to motion sickness when they were recumbent for 6 h prior to rotation. These results are countervidence for the hypothesis that shifts of body fluid are responsible in large part for the motion sickness elicited in orbital space flight."}
{"id": "PMID:464963", "title": "Saccadic velocity characteristics: intrinsic variability and fatigue.", "content": "Saccadic eye movements exhibit a characteristic peak velocity vs. amplitude relationship. As with all quantifications of biological function, there exists and associated intra- and intersubject variability of this relationship. This paper documents this variability and demonstrates both the absence of a predictable short-term \"muscle fatigue\" effect and the presence of a generalized \"mental fatigue\" (i.e. tiredness) effect.", "contents": "Saccadic velocity characteristics: intrinsic variability and fatigue. Saccadic eye movements exhibit a characteristic peak velocity vs. amplitude relationship. As with all quantifications of biological function, there exists and associated intra- and intersubject variability of this relationship. This paper documents this variability and demonstrates both the absence of a predictable short-term \"muscle fatigue\" effect and the presence of a generalized \"mental fatigue\" (i.e. tiredness) effect."}
{"id": "PMID:464964", "title": "A study of the health status of radar workers.", "content": "The health status was examined of 322 radar workers with a history of occupational exposure in a microwave field from 5-10 years. The clinical findings were compared with a control group of 220 persons. There were no statistically significant differences in clinical and laboratory findings between exposed and control group. Only some subjective complaints were found more frequently in the exposed group than in the control. The authors' feeling is that subjective complaints in radar workers could not be ascribed only to the influence of microwaves because they could be due to other nonspecific harmful occupational factors. On the basis of the results of this work, the authors concluded that it is unlikely to expect marked harmful effects from microwaves in radar workers in normal working conditions.", "contents": "A study of the health status of radar workers. The health status was examined of 322 radar workers with a history of occupational exposure in a microwave field from 5-10 years. The clinical findings were compared with a control group of 220 persons. There were no statistically significant differences in clinical and laboratory findings between exposed and control group. Only some subjective complaints were found more frequently in the exposed group than in the control. The authors' feeling is that subjective complaints in radar workers could not be ascribed only to the influence of microwaves because they could be due to other nonspecific harmful occupational factors. On the basis of the results of this work, the authors concluded that it is unlikely to expect marked harmful effects from microwaves in radar workers in normal working conditions."}
{"id": "PMID:464965", "title": "Cerebral accident subsequent to G-force loading: a case report.", "content": "A case review of a CVA in a 57-year-old general officer due to positive G-force loading is presented, which is apparently the first such documented episode recorded in the literature. This case reflects the concern about high G-force loading seen in newer high-performance aircraft and has significance in the assignment of older individuals to high G-force environments.", "contents": "Cerebral accident subsequent to G-force loading: a case report. A case review of a CVA in a 57-year-old general officer due to positive G-force loading is presented, which is apparently the first such documented episode recorded in the literature. This case reflects the concern about high G-force loading seen in newer high-performance aircraft and has significance in the assignment of older individuals to high G-force environments."}
{"id": "PMID:464966", "title": "Survey on eye comfort in aircraft: I. Flight attendants.", "content": "Extensive research in aviation medicine has been devoted to various aspects of vision, but there has been little attention to the eye problems of flight attendants. Flight attendants, especially contact lens wearers, have complained about eye discomfort in aircraft. The purpose of this study was to evaluate some of the conditions in aircraft that might influence their eye comfort. A questionnaire on eye comfort was developed in conjunction with the Air Safety Department of the Association of Flight Attendants and distributed through its Flightlog magazine. Of the 774 respondents, 95% reported some eye discomfort in aircraft. It is significant that both those who did and who did wear contact lenses reported similar eye problems. The most common eye problems reported were conjunctival redness and dried eyes. Smoking was indicated by the respondents to be the most noticeable factor causing eye symptoms. Aircraft cabin conditions are discussed, including relative humidity, atmospheric oxygen, and ozone concentration. Since air passengers are exposed to the same aircraft conditions as the attendants, they probably would manifest similar eye problems.", "contents": "Survey on eye comfort in aircraft: I. Flight attendants. Extensive research in aviation medicine has been devoted to various aspects of vision, but there has been little attention to the eye problems of flight attendants. Flight attendants, especially contact lens wearers, have complained about eye discomfort in aircraft. The purpose of this study was to evaluate some of the conditions in aircraft that might influence their eye comfort. A questionnaire on eye comfort was developed in conjunction with the Air Safety Department of the Association of Flight Attendants and distributed through its Flightlog magazine. Of the 774 respondents, 95% reported some eye discomfort in aircraft. It is significant that both those who did and who did wear contact lenses reported similar eye problems. The most common eye problems reported were conjunctival redness and dried eyes. Smoking was indicated by the respondents to be the most noticeable factor causing eye symptoms. Aircraft cabin conditions are discussed, including relative humidity, atmospheric oxygen, and ozone concentration. Since air passengers are exposed to the same aircraft conditions as the attendants, they probably would manifest similar eye problems."}
{"id": "PMID:464967", "title": "The nursing explosion-technology, knowledge, and practice: its implications for leadership in aerospace nursing.", "content": "Authorities agree that we are experiencing a tremendous upheavel in numerous fields of endeaveor, health care in particular, resulting from the rapid growth of human information and capabilities since the 1950s. This upgrowth has touched nursing. This nursing explosion, in terms of technological advancement, increased knowledge, and expanded practice, demands the leadership of aerospace nursing to reexamine it posture and direction. A fresh look at its past and present successes and failures is essential to meet the challenges of the future. For \"not to decide is to decide\". There are two basic assumptions which underlie this challenge for leadership caused by the nursing explosion. First, as a self-directing profession, nursing can define its philosophical base, determine its goals, and identify the means for attaining those goals. Second, as the largest of the health professions, nursing has the right and responsibility to respond to the quantity and quality of global health care delivery systems. The analysis of this challenge for leadership shall be attempted in the light of three perspectives--organizationally, socio-politically, and professionally. The nursing explosion has dramatized the realization that nursing has the knowledge, power, resources in numbers, and dedication to service to move health care systems in the direction necessary. As Prof. Bell of Harvard has noted, the current revolution of rising entitlement to health care will have a marked influence on the nursing effort and vice versa. The call to leadership \"a loud call and a clear call and it cannot be denied.\"", "contents": "The nursing explosion-technology, knowledge, and practice: its implications for leadership in aerospace nursing. Authorities agree that we are experiencing a tremendous upheavel in numerous fields of endeaveor, health care in particular, resulting from the rapid growth of human information and capabilities since the 1950s. This upgrowth has touched nursing. This nursing explosion, in terms of technological advancement, increased knowledge, and expanded practice, demands the leadership of aerospace nursing to reexamine it posture and direction. A fresh look at its past and present successes and failures is essential to meet the challenges of the future. For \"not to decide is to decide\". There are two basic assumptions which underlie this challenge for leadership caused by the nursing explosion. First, as a self-directing profession, nursing can define its philosophical base, determine its goals, and identify the means for attaining those goals. Second, as the largest of the health professions, nursing has the right and responsibility to respond to the quantity and quality of global health care delivery systems. The analysis of this challenge for leadership shall be attempted in the light of three perspectives--organizationally, socio-politically, and professionally. The nursing explosion has dramatized the realization that nursing has the knowledge, power, resources in numbers, and dedication to service to move health care systems in the direction necessary. As Prof. Bell of Harvard has noted, the current revolution of rising entitlement to health care will have a marked influence on the nursing effort and vice versa. The call to leadership \"a loud call and a clear call and it cannot be denied.\""}
{"id": "PMID:464968", "title": "Space osteoporosis: an electromagnetic hypothesis.", "content": "Loss of body calcium during spaceflight is a potential problem in long voyages. This loss does not appear to be caused by a deficiency in diet or exercise. The idea is advanced that the altered electromagnetic environment experienced in space may be at least partially responsible. We show that the electric field induced inside astronauts because of their motion in the geomagnetic field is greater than that which has produced a wide variety of biological effects in earth-bound experiments.", "contents": "Space osteoporosis: an electromagnetic hypothesis. Loss of body calcium during spaceflight is a potential problem in long voyages. This loss does not appear to be caused by a deficiency in diet or exercise. The idea is advanced that the altered electromagnetic environment experienced in space may be at least partially responsible. We show that the electric field induced inside astronauts because of their motion in the geomagnetic field is greater than that which has produced a wide variety of biological effects in earth-bound experiments."}
{"id": "PMID:464990", "title": "The global energy system.", "content": "A global energy system is conceptualized and analyzed, the energy distributor sub-system of the worldwide supranational system. Its many interconnections are examined and traced back to their source to determine the major elements of this global energy system. Long-term trends are emphasized. The analysis begins with a discussion of the local systems that resulted from the deployment of technology in the mid-nineteenth century, continues with a description of the global system based on oil that has existed for the past two decades, and ends with a scenario implying that an energy transition will occur in the future in which use of coal, nuclear, and solar energy will predominate. A major problem for the future will be the management of this energy transition. The optimal use of global resources and the efficient management of this transition will require a stable and persistent global order.", "contents": "The global energy system. A global energy system is conceptualized and analyzed, the energy distributor sub-system of the worldwide supranational system. Its many interconnections are examined and traced back to their source to determine the major elements of this global energy system. Long-term trends are emphasized. The analysis begins with a discussion of the local systems that resulted from the deployment of technology in the mid-nineteenth century, continues with a description of the global system based on oil that has existed for the past two decades, and ends with a scenario implying that an energy transition will occur in the future in which use of coal, nuclear, and solar energy will predominate. A major problem for the future will be the management of this energy transition. The optimal use of global resources and the efficient management of this transition will require a stable and persistent global order."}
{"id": "PMID:465095", "title": "An evaluation of salivary scintigraphy in Sj\u00f6gren's syndrome.", "content": "Sequential salivary scintigraphy, labial salivary gland biopsy, and measurement of stimulated parotid flow rate were performed in 50 patients suspected of having Sj\u00f6gren's syndrome. The value of these tests in the diagnosis of this disease was compared. Salivary scintigraphy and labial salivary gland biopsy can be used together to diagnose the oral component of Sj\u00f6gren's syndrome. Abnormal salivary scintigraphy correlated with both of the other tests and may be considered optional in the diagnostic evaluation.", "contents": "An evaluation of salivary scintigraphy in Sj\u00f6gren's syndrome. Sequential salivary scintigraphy, labial salivary gland biopsy, and measurement of stimulated parotid flow rate were performed in 50 patients suspected of having Sj\u00f6gren's syndrome. The value of these tests in the diagnosis of this disease was compared. Salivary scintigraphy and labial salivary gland biopsy can be used together to diagnose the oral component of Sj\u00f6gren's syndrome. Abnormal salivary scintigraphy correlated with both of the other tests and may be considered optional in the diagnostic evaluation."}
{"id": "PMID:465096", "title": "The clinical significance of Raynaud's phenomenon in systemic lupus erythematosus.", "content": "In a prospective study of 226 patients with systemic lupus erythematosus (SLE), 91 patients (40%) had Raynaud's phenomenon. These patients were compared to 135 patients without Raynaud's phenomenon. Patients with Raynaud's phenomenon had a greater incidence of arthritis (P less than 0.02), malar rash (P less than 0.003), and photosensitivity (P less than 0.03), and a lesser incidence of severe renal disease as manifested by serum creatinine over 3.0 mg/dl (P less than 0.007) or creatinine clearance below 60 ml/minute. Patients with Raynaud's phenomenon were less likely to have severe, life threatening disease and received a lower average monthly (P less than 0.01) and a lower peak daily corticosteroid dose (P less than 0.01). Fourteen patients (16%) with Raynaud's phenomenon died, compared to 41 without (30%) (P less than 0.03). Raynaud's phenomenon in patients with SLE is associated with milder disease and may be regarded as a favorable prognostic sign.", "contents": "The clinical significance of Raynaud's phenomenon in systemic lupus erythematosus. In a prospective study of 226 patients with systemic lupus erythematosus (SLE), 91 patients (40%) had Raynaud's phenomenon. These patients were compared to 135 patients without Raynaud's phenomenon. Patients with Raynaud's phenomenon had a greater incidence of arthritis (P less than 0.02), malar rash (P less than 0.003), and photosensitivity (P less than 0.03), and a lesser incidence of severe renal disease as manifested by serum creatinine over 3.0 mg/dl (P less than 0.007) or creatinine clearance below 60 ml/minute. Patients with Raynaud's phenomenon were less likely to have severe, life threatening disease and received a lower average monthly (P less than 0.01) and a lower peak daily corticosteroid dose (P less than 0.01). Fourteen patients (16%) with Raynaud's phenomenon died, compared to 41 without (30%) (P less than 0.03). Raynaud's phenomenon in patients with SLE is associated with milder disease and may be regarded as a favorable prognostic sign."}
{"id": "PMID:465097", "title": "Pharmacokinetic monitoring of salicylate therapy in children with juvenile rheumatoid arthritis.", "content": "Free and total (sum of free and protein bound) salicylate concentrations in serum were determined in 17 children (age: 4-17 years) with definite juvenile rheumatoid arthritis. These measurements were carried out immediately before and 2, 4, and 8 hours after the morning dose during a strict 8 hourly aspirin treatment regimen (regular tablets) started 5 days earlier. The ratio of the 0 to 8 hour total salicylate concentrations was 0.95 +/- 0.10 (mean +/- SD), indicating that steady state had been attained. The ratio of the maximum to minimum concentrations during the dosing interval ranged from 1.05 to 2.26 and decreased with increasing average concentration. The concentration ratio was less than 1.3 at average salicylate concentrations above 20 mg/100 ml. It is concluded that the timing of a blood sample is not critical for monitoring steady state serum salicylate concentrations in the usual therapeutic range if the dosing interval is 8 hours or less. Free salicylate concentrations increased more than proportionately with increasing total concentrations due to the concentration dependent protein binding of the drug in serum.", "contents": "Pharmacokinetic monitoring of salicylate therapy in children with juvenile rheumatoid arthritis. Free and total (sum of free and protein bound) salicylate concentrations in serum were determined in 17 children (age: 4-17 years) with definite juvenile rheumatoid arthritis. These measurements were carried out immediately before and 2, 4, and 8 hours after the morning dose during a strict 8 hourly aspirin treatment regimen (regular tablets) started 5 days earlier. The ratio of the 0 to 8 hour total salicylate concentrations was 0.95 +/- 0.10 (mean +/- SD), indicating that steady state had been attained. The ratio of the maximum to minimum concentrations during the dosing interval ranged from 1.05 to 2.26 and decreased with increasing average concentration. The concentration ratio was less than 1.3 at average salicylate concentrations above 20 mg/100 ml. It is concluded that the timing of a blood sample is not critical for monitoring steady state serum salicylate concentrations in the usual therapeutic range if the dosing interval is 8 hours or less. Free salicylate concentrations increased more than proportionately with increasing total concentrations due to the concentration dependent protein binding of the drug in serum."}
{"id": "PMID:465098", "title": "Ophthalmologic safety of long-term hydroxychloroquine treatment.", "content": "Ocular toxicity of long-term hydroxychloroquine treatment was assessed by regular ophthalmologic examinations in 99 patients. No patient developed significant loss of vision or visual field constriction to a white test object. Three patients had evidence of toxicity, but the medication had to be permanently discontinued in only one who subsequently had regression of all abnormalities except visual field constriction to a red test object. Neither duration of treatment nor the diagnosis of systemic lupus erythematosus predisposed patients to toxicity.", "contents": "Ophthalmologic safety of long-term hydroxychloroquine treatment. Ocular toxicity of long-term hydroxychloroquine treatment was assessed by regular ophthalmologic examinations in 99 patients. No patient developed significant loss of vision or visual field constriction to a white test object. Three patients had evidence of toxicity, but the medication had to be permanently discontinued in only one who subsequently had regression of all abnormalities except visual field constriction to a red test object. Neither duration of treatment nor the diagnosis of systemic lupus erythematosus predisposed patients to toxicity."}
{"id": "PMID:465099", "title": "Quantitative sacroiliac joint scintigraphy. A critical assessment.", "content": "With quantitative sacroiliac joint scintigraphy (QSS), sacroiliac joint-to-sacrum (SIS) ratios greater than or equal to 1.40 are abnormal for our method. High SIS ratios are associated with roentgenographically appreciated sacroiliitis and with early clinically evident sacroiliitis. Although highly sensitive, QSS abnormalities are not specific for inflammatory sacroiliac (SI) joint disease. High SIS ratios are also seen in rheumatoid arthritis, degenerative abnormalities of the lumbosacral spine, and metabolic bone disorder. Other disorders with roentgenographic SI joint abnormalities must be studied scintigraphically before QSS can be employed as a useful diagnostic test.", "contents": "Quantitative sacroiliac joint scintigraphy. A critical assessment. With quantitative sacroiliac joint scintigraphy (QSS), sacroiliac joint-to-sacrum (SIS) ratios greater than or equal to 1.40 are abnormal for our method. High SIS ratios are associated with roentgenographically appreciated sacroiliitis and with early clinically evident sacroiliitis. Although highly sensitive, QSS abnormalities are not specific for inflammatory sacroiliac (SI) joint disease. High SIS ratios are also seen in rheumatoid arthritis, degenerative abnormalities of the lumbosacral spine, and metabolic bone disorder. Other disorders with roentgenographic SI joint abnormalities must be studied scintigraphically before QSS can be employed as a useful diagnostic test."}
{"id": "PMID:465100", "title": "Deposition of antibodies to a soluble cytoplasmic antigen in the kidneys of patients with systemic lupus erythematosus.", "content": "Two patients with systemic lupus erythematosus were studied whose sera contained precipitating antibodies to a soluble cytoplasmic antigen, termed Ro. A reduction in the amount of these antibodies in each case was accompanied by a deterioration in the clinical status with the development of nephritis leading to death. Acid elution of gamma globulin was performed from homogenates of the renal cortex, and in both instances antibodies to Ro were demonstrated in the eluates by double immunodiffusion. The titer of these antibodies was measured in both sera and eluates, and specific enrichment of anti-Ro in the eluates was demonstrated in both patients. This strongly suggests the direct participation of Ro-anti-Ro immune complexes in the progressive renal disease and may underlie the association seen here between the decreasing serum titers of antibodies to Ro and the clinical deterioration in these two cases.", "contents": "Deposition of antibodies to a soluble cytoplasmic antigen in the kidneys of patients with systemic lupus erythematosus. Two patients with systemic lupus erythematosus were studied whose sera contained precipitating antibodies to a soluble cytoplasmic antigen, termed Ro. A reduction in the amount of these antibodies in each case was accompanied by a deterioration in the clinical status with the development of nephritis leading to death. Acid elution of gamma globulin was performed from homogenates of the renal cortex, and in both instances antibodies to Ro were demonstrated in the eluates by double immunodiffusion. The titer of these antibodies was measured in both sera and eluates, and specific enrichment of anti-Ro in the eluates was demonstrated in both patients. This strongly suggests the direct participation of Ro-anti-Ro immune complexes in the progressive renal disease and may underlie the association seen here between the decreasing serum titers of antibodies to Ro and the clinical deterioration in these two cases."}
{"id": "PMID:465101", "title": "Simultaneous immunologic studies of skin and kidney in systemic lupus erythematosus clinicopathologic correlations.", "content": "Thirty-two biopsies of kidneys and of normal skin were performed simultaneously on patients with systemic lupus erythematosus (SLE) to determine whether the deposition of immune reactants in skin was correlated with the severity of renal injury or with several serologic measures of systemic disease activity. Immunofluorescent deposits at the dermal-epidermal junction (lupus band test) did not correlate with any clinical or histologic measures of glomerulonephritis or with serologic abnormalities. Immune deposits in dermal venules were found in 56% of the skin biopsies and were correlated with hypocomplementemia and higher levels of immune complexes in serum, and possibly with glomerular subendothelial electron dense deposits. Azathioprine therapy was correlated with absence of both sub-epidermal and vascular immune deposits. Immuno-fluorescent findings on serial skin biopsies on 13 patients were highly inconsistent. It is concluded that the lupus band test is clinically useful only as a diagnostic aid but not helpful in assessing renal or serologic activity of lupus, and that dermal vascular deposits of immunoglobulin or complement are more frequent than previoulsy recognized and correlate with measures of circulating immune complexes.", "contents": "Simultaneous immunologic studies of skin and kidney in systemic lupus erythematosus clinicopathologic correlations. Thirty-two biopsies of kidneys and of normal skin were performed simultaneously on patients with systemic lupus erythematosus (SLE) to determine whether the deposition of immune reactants in skin was correlated with the severity of renal injury or with several serologic measures of systemic disease activity. Immunofluorescent deposits at the dermal-epidermal junction (lupus band test) did not correlate with any clinical or histologic measures of glomerulonephritis or with serologic abnormalities. Immune deposits in dermal venules were found in 56% of the skin biopsies and were correlated with hypocomplementemia and higher levels of immune complexes in serum, and possibly with glomerular subendothelial electron dense deposits. Azathioprine therapy was correlated with absence of both sub-epidermal and vascular immune deposits. Immuno-fluorescent findings on serial skin biopsies on 13 patients were highly inconsistent. It is concluded that the lupus band test is clinically useful only as a diagnostic aid but not helpful in assessing renal or serologic activity of lupus, and that dermal vascular deposits of immunoglobulin or complement are more frequent than previoulsy recognized and correlate with measures of circulating immune complexes."}
{"id": "PMID:465102", "title": "Glycosaminoglycan synthesis and glucose uptake by rheumatoid and nonrheumatoid fibroblastic cells in culture. The effect of nutritional factors.", "content": "Glycosaminoglycan (GAG) synthesis and glucose uptake by rheumatoid (R) and nonrheumatoid (NR) synovial cells were studied at the second subculture during four different sets of nutritional conditions and at sequential, defined intervals of the growth cycle. Synovial fibroblastic cells in monolayer cultures secrete both hyaluronic acid and sulfated GAGs in a ratio of about 8 : 1. With increasing cell density the ability to sulfate GAGs appears to decrease. No significant differences in GAG synthesis between rheumatoid and nonrheumatoid lines on a per cell basis could be detected during any interval in the growth cycle. Similarly, no significant differences of glucose uptake per cell could be demonstrated between rheumatoid and nonrheumatoid lines under the various protocols applied throughout the growth cycle. The most important factor determining glucose uptake per cell is the availability of glucose in the medium which is in turn closely correlated with cell number.", "contents": "Glycosaminoglycan synthesis and glucose uptake by rheumatoid and nonrheumatoid fibroblastic cells in culture. The effect of nutritional factors. Glycosaminoglycan (GAG) synthesis and glucose uptake by rheumatoid (R) and nonrheumatoid (NR) synovial cells were studied at the second subculture during four different sets of nutritional conditions and at sequential, defined intervals of the growth cycle. Synovial fibroblastic cells in monolayer cultures secrete both hyaluronic acid and sulfated GAGs in a ratio of about 8 : 1. With increasing cell density the ability to sulfate GAGs appears to decrease. No significant differences in GAG synthesis between rheumatoid and nonrheumatoid lines on a per cell basis could be detected during any interval in the growth cycle. Similarly, no significant differences of glucose uptake per cell could be demonstrated between rheumatoid and nonrheumatoid lines under the various protocols applied throughout the growth cycle. The most important factor determining glucose uptake per cell is the availability of glucose in the medium which is in turn closely correlated with cell number."}
{"id": "PMID:465111", "title": "Hyperlipoproteinemia induced by a transplantable pituitary tumor in the rat.", "content": "The MtT-F4 tumor, a transplantable pituitary tumor of rats, induces significant hyperlipidemia in male Fisher 344 rats. The increasive hypercholesterolemia was accompanied by hypertriglyceridemia only in the first month of tumor implantation. Clofibrate feeding inhibited the development of hypercholesterolemia and maintained normal serum triglyceride levels. In contrast to the changes in lipoprotein cholesterol distribution and profile found in experimental hyperlipidemia induced by high fat and cholesterol feeding, the hypercholesterolemic tumor-bearing rats showed no accumulation of cholesterol in the very low density and intermediate density lipoproteins, and no appearance of a new class of lipoprotein, B-VLDL. An HDLc-like lipoprotein appeared as hypercholesterolemia developed. Increased amounts of cholesterol were deposited in the aorta. The effects are attributed to the lipolytic hormones secreted by the tumor and antagonism to their action by clofibrate.", "contents": "Hyperlipoproteinemia induced by a transplantable pituitary tumor in the rat. The MtT-F4 tumor, a transplantable pituitary tumor of rats, induces significant hyperlipidemia in male Fisher 344 rats. The increasive hypercholesterolemia was accompanied by hypertriglyceridemia only in the first month of tumor implantation. Clofibrate feeding inhibited the development of hypercholesterolemia and maintained normal serum triglyceride levels. In contrast to the changes in lipoprotein cholesterol distribution and profile found in experimental hyperlipidemia induced by high fat and cholesterol feeding, the hypercholesterolemic tumor-bearing rats showed no accumulation of cholesterol in the very low density and intermediate density lipoproteins, and no appearance of a new class of lipoprotein, B-VLDL. An HDLc-like lipoprotein appeared as hypercholesterolemia developed. Increased amounts of cholesterol were deposited in the aorta. The effects are attributed to the lipolytic hormones secreted by the tumor and antagonism to their action by clofibrate."}
{"id": "PMID:465112", "title": "Cathepsin D activity of venous and polyester vascular grafts implanted in the abdominal aorta of the dog.", "content": "Autogenous veins and polyester prostheses were implanted in the abdominal aorta of dogs. The venous or polyester grafts were removed together with sections of the normal arteries and veins 1 month, 4 months, and 3 years after the operation. This material was then dissected into the intimal, medial, and adventitial layers, and homogenized after which their cathepsin D activity was determined. It was found that the cathepsin D activity was far higher in all the layers of the venous grafts, particularly in the polyester grafts, than in the corresponding layers of the normal arteries and veins.", "contents": "Cathepsin D activity of venous and polyester vascular grafts implanted in the abdominal aorta of the dog. Autogenous veins and polyester prostheses were implanted in the abdominal aorta of dogs. The venous or polyester grafts were removed together with sections of the normal arteries and veins 1 month, 4 months, and 3 years after the operation. This material was then dissected into the intimal, medial, and adventitial layers, and homogenized after which their cathepsin D activity was determined. It was found that the cathepsin D activity was far higher in all the layers of the venous grafts, particularly in the polyester grafts, than in the corresponding layers of the normal arteries and veins."}
{"id": "PMID:465113", "title": "Effect of clofibrate and gemfibrozil on the activities of mitochondrial carnitine acyltransferases in rat liver. Dose--response relations.", "content": "The effects of different doses of clofibrate and gemfibrozil on liver size, serum triglyceride concentration and the activities of hepatic mitochondrial alpha-glycerophosphate dehydrogenase (alpha-GPD) and carnitine acyltransferases were studied in male rats. Both clofibrate and gemfibrozil treatment effectively decreased the fructose-induced hypertriglyceridaemia and increased the liver to body weight ratio. Clofibrate treatment also induced an increase of many times in the activities of mitochondrial alpha-GPD and carnitine acyltransferases, the effect increasing with the dose used. The effect of gemfibrozil on the activities of the enzymes was significantly smaller. There was no correlation between the decrease in serum triglyceride concentration and the changes in the activities of the enzymes. Only clofibrate increased the rate of fatty acylcarnitine oxidation in isolated mitochondria. It is concluded that both drugs increased the size of the rat liver, but that only clofibrate influenced the mitochondrial enzyme activities of mitochondrial carnitine acyltransferases and the accelerated mitochondrial oxidation of fatty acids are not the mechanisms by which these drugs lower serum lipid levels.", "contents": "Effect of clofibrate and gemfibrozil on the activities of mitochondrial carnitine acyltransferases in rat liver. Dose--response relations. The effects of different doses of clofibrate and gemfibrozil on liver size, serum triglyceride concentration and the activities of hepatic mitochondrial alpha-glycerophosphate dehydrogenase (alpha-GPD) and carnitine acyltransferases were studied in male rats. Both clofibrate and gemfibrozil treatment effectively decreased the fructose-induced hypertriglyceridaemia and increased the liver to body weight ratio. Clofibrate treatment also induced an increase of many times in the activities of mitochondrial alpha-GPD and carnitine acyltransferases, the effect increasing with the dose used. The effect of gemfibrozil on the activities of the enzymes was significantly smaller. There was no correlation between the decrease in serum triglyceride concentration and the changes in the activities of the enzymes. Only clofibrate increased the rate of fatty acylcarnitine oxidation in isolated mitochondria. It is concluded that both drugs increased the size of the rat liver, but that only clofibrate influenced the mitochondrial enzyme activities of mitochondrial carnitine acyltransferases and the accelerated mitochondrial oxidation of fatty acids are not the mechanisms by which these drugs lower serum lipid levels."}
{"id": "PMID:465114", "title": "Distribution and movement of anionic cell surface sites in cultured human vascular endothelial cells.", "content": "Cell surface anionic sites on primary and transformed cultures of human vascular endothelium were studied using cationized ferritin (CF) as an ultrastructural marker. The native distribution of anionic sites on the upper (free) surfaces of cells, fixed in situ with glutaraldehyde, was uniform. Binding of the polycationic ligand, CF, in unfixed cells induced redistribution of anionic sites. Rapid formation of discrete patches of CF particles was followed by reappearance of binding between patches, movement of surface-bound CF into intercellular clefts, and endocytosis, over the next 30 min. Aldehyde-fixed cells, detached from the culture surface, bound CF on both upper and lower surfaces. The distribution and mobility of negatively charged membrane components in vascular endothelium may have relevance for thrombosis, atherogenesis, and vascular permeability.", "contents": "Distribution and movement of anionic cell surface sites in cultured human vascular endothelial cells. Cell surface anionic sites on primary and transformed cultures of human vascular endothelium were studied using cationized ferritin (CF) as an ultrastructural marker. The native distribution of anionic sites on the upper (free) surfaces of cells, fixed in situ with glutaraldehyde, was uniform. Binding of the polycationic ligand, CF, in unfixed cells induced redistribution of anionic sites. Rapid formation of discrete patches of CF particles was followed by reappearance of binding between patches, movement of surface-bound CF into intercellular clefts, and endocytosis, over the next 30 min. Aldehyde-fixed cells, detached from the culture surface, bound CF on both upper and lower surfaces. The distribution and mobility of negatively charged membrane components in vascular endothelium may have relevance for thrombosis, atherogenesis, and vascular permeability."}
{"id": "PMID:465115", "title": "Effect of enhancement of cholesterol degradation during neonatal life of guinea pig on its subsequent response to dietary cholesterol.", "content": "The effect of feeding cholestyramine to neonatal guinea pigs on their subsequent plasma cholesterol levels and response to dietary cholesterol were studied. Male neonatal guinea pigs were suckled for 6 days. One group was maintained on a 1.1% cholestyramine diet for 6 weeks and the control group weaned normally. Both groups of guinea pigs were then fed a standard diet of Guinea Pig Chow for 6 weeks. During the standard diet period bile acid and neutral sterol excretion rates were significantly higher in the group previously treated with cholestyramine than the control group despite the similarity in plasma cholesterol levels. When both groups of guinea pigs were subjected to a 0.5% cholesterol diet for 4 weeks, plasma cholesterol levels were significantly lower in the group previously treated with cholestyramine than the control group. The plasma cholesterol levels continued to be significantly lower in the group previously treated with cholestyramine after an additional four weeks on standard diet. These results suggest that stimulation of cholesterol catabolism in the neonatal period can influence the subsequent response to dietary cholesterol.", "contents": "Effect of enhancement of cholesterol degradation during neonatal life of guinea pig on its subsequent response to dietary cholesterol. The effect of feeding cholestyramine to neonatal guinea pigs on their subsequent plasma cholesterol levels and response to dietary cholesterol were studied. Male neonatal guinea pigs were suckled for 6 days. One group was maintained on a 1.1% cholestyramine diet for 6 weeks and the control group weaned normally. Both groups of guinea pigs were then fed a standard diet of Guinea Pig Chow for 6 weeks. During the standard diet period bile acid and neutral sterol excretion rates were significantly higher in the group previously treated with cholestyramine than the control group despite the similarity in plasma cholesterol levels. When both groups of guinea pigs were subjected to a 0.5% cholesterol diet for 4 weeks, plasma cholesterol levels were significantly lower in the group previously treated with cholestyramine than the control group. The plasma cholesterol levels continued to be significantly lower in the group previously treated with cholestyramine after an additional four weeks on standard diet. These results suggest that stimulation of cholesterol catabolism in the neonatal period can influence the subsequent response to dietary cholesterol."}
{"id": "PMID:465117", "title": "The effect of a putative anti-atherosclerotic agent (S 1204) on lipid metabolism in rabbit aorta.", "content": "The effects of the novel fenfluramine derivative, S 1204 (meta-trifluoromethyl phenyl-1 [beta(sulfamyl-3'-chloro-4'-benzoyloxyethyl)]amino-2-propane) were studied on lipid metabolism in rabbit aorta and other tissues. Pretreatment of rabbits with S 1204 (50 mg/kg orally) for 10 days strongly inhibited the aortic incorporation of an intravenous 20 muCi tracer-dose of [4-(14)C]-cholesterol given 24 h earlier. However, incorporation of such cholesterol radioactivity into the liver, intestine, lung or plasma was not affected, in comparison with control animals. One hour after i.v. injection of a 100 muCi tracer-dose of [2-(14)C]acetate, S 1204 significantly reduced the radioactivity of total lipids in the aorta and liver. Such inhibition of acetate incorporation into arterial lipids was observed with all lipid fractions (that is, free fatty acids, esterified cholesterol, especially phospholipids, glycerides and free cholesterol). Incorporation of acetate into intestinal triglyceride fractions was also significantly decreased. The results indicate that S 1204 may have anti-atherogenic properties, which could be valuable in the clinical treatment of atherosclerosis.", "contents": "The effect of a putative anti-atherosclerotic agent (S 1204) on lipid metabolism in rabbit aorta. The effects of the novel fenfluramine derivative, S 1204 (meta-trifluoromethyl phenyl-1 [beta(sulfamyl-3'-chloro-4'-benzoyloxyethyl)]amino-2-propane) were studied on lipid metabolism in rabbit aorta and other tissues. Pretreatment of rabbits with S 1204 (50 mg/kg orally) for 10 days strongly inhibited the aortic incorporation of an intravenous 20 muCi tracer-dose of [4-(14)C]-cholesterol given 24 h earlier. However, incorporation of such cholesterol radioactivity into the liver, intestine, lung or plasma was not affected, in comparison with control animals. One hour after i.v. injection of a 100 muCi tracer-dose of [2-(14)C]acetate, S 1204 significantly reduced the radioactivity of total lipids in the aorta and liver. Such inhibition of acetate incorporation into arterial lipids was observed with all lipid fractions (that is, free fatty acids, esterified cholesterol, especially phospholipids, glycerides and free cholesterol). Incorporation of acetate into intestinal triglyceride fractions was also significantly decreased. The results indicate that S 1204 may have anti-atherogenic properties, which could be valuable in the clinical treatment of atherosclerosis."}
{"id": "PMID:465116", "title": "Effect of CCL4-induced cirrhosis on the pathophysiologic course of acute myocardial infarction in nonarteriosclerotic vs arteriosclerotic male rats.", "content": "Arteriosclerotic and nonarteriosclerotic rats were treated with carbon tetrachloride (CCL4) to induce cirrhosis of the liver. Massive myocardial infarction was then induced in intact and CCL4-treated animals. During acute necrosis (Days 1 thru 3), animals were killed at 4, 8, 12 and 24 h on Days 1 and 2, and during myocardial repair on Days 4, 5 and 8. During the induction of cirrhosis, animals developed polydypsia, polyuria, and hyperglycemia; during myocardial infarction, the arteriosclerotic + cirrhotic animals developed severe and persistent congestive heart failure, i.e., hydrothorax. Adrenal and thymus gland weights and corticosterone levels indicated that cirrhosis per se increased pituitary--adrenal activity, particularly in arteriosclerotic animals. Enzyme levels of SGOT and SGPT demonstrated severe hepatic damage due to cirrhosis and acute myocardial infarction. Blood triglycerides and cholesterol responded abnormally in cirrhotic animals during acute myocardial ischemia due to their entrapment within hepatic cells. The cirrhotic animals manifested poor myocardial repair with persistent foci of necrosis, calcification, and a high incidence of large, occlusive, atrial thrombi. It is suggested that cirrhosis interferes with lipid metabolism and adrenal steroid conjugation leading to abnormal levels of mineralocorticoids which favor congestive heart failure, poor myocardial repair, and atrial thrombosis.", "contents": "Effect of CCL4-induced cirrhosis on the pathophysiologic course of acute myocardial infarction in nonarteriosclerotic vs arteriosclerotic male rats. Arteriosclerotic and nonarteriosclerotic rats were treated with carbon tetrachloride (CCL4) to induce cirrhosis of the liver. Massive myocardial infarction was then induced in intact and CCL4-treated animals. During acute necrosis (Days 1 thru 3), animals were killed at 4, 8, 12 and 24 h on Days 1 and 2, and during myocardial repair on Days 4, 5 and 8. During the induction of cirrhosis, animals developed polydypsia, polyuria, and hyperglycemia; during myocardial infarction, the arteriosclerotic + cirrhotic animals developed severe and persistent congestive heart failure, i.e., hydrothorax. Adrenal and thymus gland weights and corticosterone levels indicated that cirrhosis per se increased pituitary--adrenal activity, particularly in arteriosclerotic animals. Enzyme levels of SGOT and SGPT demonstrated severe hepatic damage due to cirrhosis and acute myocardial infarction. Blood triglycerides and cholesterol responded abnormally in cirrhotic animals during acute myocardial ischemia due to their entrapment within hepatic cells. The cirrhotic animals manifested poor myocardial repair with persistent foci of necrosis, calcification, and a high incidence of large, occlusive, atrial thrombi. It is suggested that cirrhosis interferes with lipid metabolism and adrenal steroid conjugation leading to abnormal levels of mineralocorticoids which favor congestive heart failure, poor myocardial repair, and atrial thrombosis."}
{"id": "PMID:465118", "title": "Reduction of blood viscosity following plasma exchange.", "content": "The effect of plasma exchange with plasma protein fraction on blood viscosity was determined in seven hyperlipoproteinaemic patients with coronary or peripheral vascular disease. This resulted in decreases in whole blood viscosity of 83% and 30% respectively at the lowest and highest shear rates studied, and decreases of 21% and 59% in plasma viscosity and fibrinogen. Serum cholesterol and triglyceride were reduced by 66% and 48% respectively. Sequential studies in two patients showed that blood viscosity returned to near-basal values by the 6th day. These findings suggest that plasma exchange may result in short-term enhancement of blood flow in vessels where low shear rates predominate.", "contents": "Reduction of blood viscosity following plasma exchange. The effect of plasma exchange with plasma protein fraction on blood viscosity was determined in seven hyperlipoproteinaemic patients with coronary or peripheral vascular disease. This resulted in decreases in whole blood viscosity of 83% and 30% respectively at the lowest and highest shear rates studied, and decreases of 21% and 59% in plasma viscosity and fibrinogen. Serum cholesterol and triglyceride were reduced by 66% and 48% respectively. Sequential studies in two patients showed that blood viscosity returned to near-basal values by the 6th day. These findings suggest that plasma exchange may result in short-term enhancement of blood flow in vessels where low shear rates predominate."}
{"id": "PMID:465119", "title": "Effect of milk constituents on hepatic cholesterol-genesis.", "content": "Two preparations active in reducing hepatic cholesterol biosynthesis were isolated from bovine skim milk. One of the inhibitors was in the dialysate and was identified as orotic acid (OA). The other inhibitor, present in the retentate, was not identified. Orotic acid appears to act by inhibiting cholesterol biosynthesis before the formation of mevalonate, whereas the retentate inhibitor exerts its effect beyond the formation of mevalonate in the biosynthetic pathway. Human milk also inhibited the incorporation of both labeled acetate and mevalonate into cholesterol by rat liver. Orotic acid was not detectable in human milk samples employed in this study. Administration of [6-14C]orotate to rats revealed its conversion to uracil in the liver. Subsequent work demonstrated that uracil had inhibitory activity on hepatic cholesterol biosynthesis similar to that of orotate when incubated with rat liver slices.", "contents": "Effect of milk constituents on hepatic cholesterol-genesis. Two preparations active in reducing hepatic cholesterol biosynthesis were isolated from bovine skim milk. One of the inhibitors was in the dialysate and was identified as orotic acid (OA). The other inhibitor, present in the retentate, was not identified. Orotic acid appears to act by inhibiting cholesterol biosynthesis before the formation of mevalonate, whereas the retentate inhibitor exerts its effect beyond the formation of mevalonate in the biosynthetic pathway. Human milk also inhibited the incorporation of both labeled acetate and mevalonate into cholesterol by rat liver. Orotic acid was not detectable in human milk samples employed in this study. Administration of [6-14C]orotate to rats revealed its conversion to uracil in the liver. Subsequent work demonstrated that uracil had inhibitory activity on hepatic cholesterol biosynthesis similar to that of orotate when incubated with rat liver slices."}
{"id": "PMID:465120", "title": "Biosynthesis of aortic collagen and glycosaminoglycan following immunological injury.", "content": "The effect of immunological injury upon the early in vivo changes in aortic connective tissue metabolism was studied. It was found that a single antigen (bovine serum albumin), when administered in multiple doses, activated collagen synthesis and increased the rate of lgycosaminoglycan synthesis in the arterial wall. The degree of stimulation of both connective tissue components was higher in animals receiving a higher dose of antigen.", "contents": "Biosynthesis of aortic collagen and glycosaminoglycan following immunological injury. The effect of immunological injury upon the early in vivo changes in aortic connective tissue metabolism was studied. It was found that a single antigen (bovine serum albumin), when administered in multiple doses, activated collagen synthesis and increased the rate of lgycosaminoglycan synthesis in the arterial wall. The degree of stimulation of both connective tissue components was higher in animals receiving a higher dose of antigen."}
{"id": "PMID:465122", "title": "Seasonal variations in the biophysical properties of rabbit aorta and its susceptibility to arteriosclerosis.", "content": "The physiological variations in the mechanical properties of rabbit aortae in relation to the periods of hair shedding were studied. The load-strain curves of the eight proximal thoracic segment in 15 shedding and 15 non-shedding male albino rabbits were analysed. The slope of the curves (tangent of the angle between the linear region of the load-strain curve and the strain axis) was decreased in the shedding animals compared to that of the non-shedding animals and the toe of the load-strain curves was significantly lower towards the x-axis in the shedding animals. The observations indicate a lower stiffness, that is increased elasticity, of the aortae of rabbits during hair shedding. The increased elasticity during hair shedding may explain the previously reported resistance to experimental arteriosclerosis caused by the hemodynamic strain elicited by exposure to systemic hypoxia. A decrease in the aortic content of collagen and of sulfated glycosaminoglycans, and an increase in the content of hyaluronic acid, may be of importance in the alterations of the mechanical properties of rabbit aorta  during hair shedding.", "contents": "Seasonal variations in the biophysical properties of rabbit aorta and its susceptibility to arteriosclerosis. The physiological variations in the mechanical properties of rabbit aortae in relation to the periods of hair shedding were studied. The load-strain curves of the eight proximal thoracic segment in 15 shedding and 15 non-shedding male albino rabbits were analysed. The slope of the curves (tangent of the angle between the linear region of the load-strain curve and the strain axis) was decreased in the shedding animals compared to that of the non-shedding animals and the toe of the load-strain curves was significantly lower towards the x-axis in the shedding animals. The observations indicate a lower stiffness, that is increased elasticity, of the aortae of rabbits during hair shedding. The increased elasticity during hair shedding may explain the previously reported resistance to experimental arteriosclerosis caused by the hemodynamic strain elicited by exposure to systemic hypoxia. A decrease in the aortic content of collagen and of sulfated glycosaminoglycans, and an increase in the content of hyaluronic acid, may be of importance in the alterations of the mechanical properties of rabbit aorta  during hair shedding."}
{"id": "PMID:465124", "title": "The arterial wall in malignant disease.", "content": "The intimal thickening of arteries present in 95 resected specimens of carcinoma of the colon was studied, using sections from the tumour area and from adjacent normal bowel. Arteries from the tumour area showed a very significant increase in intimal thickening as compared with the controls. It is suggested that the local factor associated with the tumour area is an impaired drainage of macromolecules from the intimal compartment of the arteries due to obstruction of the lymphatic channels of the surrounding interstitial tissue by tumour cells and fibrosis.", "contents": "The arterial wall in malignant disease. The intimal thickening of arteries present in 95 resected specimens of carcinoma of the colon was studied, using sections from the tumour area and from adjacent normal bowel. Arteries from the tumour area showed a very significant increase in intimal thickening as compared with the controls. It is suggested that the local factor associated with the tumour area is an impaired drainage of macromolecules from the intimal compartment of the arteries due to obstruction of the lymphatic channels of the surrounding interstitial tissue by tumour cells and fibrosis."}
{"id": "PMID:465125", "title": "Platelet aggregation in relationship to plasma catecholamines in patients with hypertension.", "content": "Plasma catecholamine concentration and platelet aggregation were studied in 22 patients with uncomplicated primary hypertension and 13 age-matched normotensive, healthy subjects at rest and in some during isometric handgrip exercise. The effect of norepinephrine (NE) infusion upon platelet aggregation was also examined. Plasma catecholamine concentration was slightly higher in the hypertensive than the normotensive group, but the difference was not significant. However, platelet aggregation to ADP was significantly greater in the hypertensive than the normotensive subjects. Exercise increased significantly both catecholamines and aggregation in both groups. Platelet aggregation was correlated with age (r = 0.62, P less than 0.01) and plasma NE (r = -0.34, P less than 0.05 for the total group of subjects). The infusion of NE increased significantly plasma NE and platelet aggregation and there was an inverse correlation between NE increase and threshold decrease (r = -0.69, P less than 0.05). Thus, plasma catecholamines and important determinants of platelet aggregation. However, in our study, uncomplicated primary hypertension was not associated with abnormal plasma catecholamine concentration. It is likely that the observed abnormal platelet aggregability to ADP represents a secondary phenomenon, possibly related to more advanced atherosclerotic vascular changes in hypertensive than normotensive subjects.", "contents": "Platelet aggregation in relationship to plasma catecholamines in patients with hypertension. Plasma catecholamine concentration and platelet aggregation were studied in 22 patients with uncomplicated primary hypertension and 13 age-matched normotensive, healthy subjects at rest and in some during isometric handgrip exercise. The effect of norepinephrine (NE) infusion upon platelet aggregation was also examined. Plasma catecholamine concentration was slightly higher in the hypertensive than the normotensive group, but the difference was not significant. However, platelet aggregation to ADP was significantly greater in the hypertensive than the normotensive subjects. Exercise increased significantly both catecholamines and aggregation in both groups. Platelet aggregation was correlated with age (r = 0.62, P less than 0.01) and plasma NE (r = -0.34, P less than 0.05 for the total group of subjects). The infusion of NE increased significantly plasma NE and platelet aggregation and there was an inverse correlation between NE increase and threshold decrease (r = -0.69, P less than 0.05). Thus, plasma catecholamines and important determinants of platelet aggregation. However, in our study, uncomplicated primary hypertension was not associated with abnormal plasma catecholamine concentration. It is likely that the observed abnormal platelet aggregability to ADP represents a secondary phenomenon, possibly related to more advanced atherosclerotic vascular changes in hypertensive than normotensive subjects."}
{"id": "PMID:465128", "title": "Elastin--lipid interaction action in the arterial wall. Part 1. Extraction of elastin from human aortic intima.", "content": "Elastin was extracted from human aortic plaque and adjacent grossly normal intima by the following methods: (1) 0.1 N NaOH at 100 degrees C, (2) hot NaOH and 0.2 M EDTA, (3) 5 M guanidine--HCl and collagenase, (4) guanidine--collagenase and dithioerythritol--urea--sodium dodecyl sulfate, (5) guanidine--collagenase and EDTA, (6) 10% NaCl and collagenase, and (7) NaCl--collagenase and EDTA. All elastin samples contained small amounts of carbohydrate and hydroxyproline. The lipid content of non-plaque intimal elastin samples was small (2--3%), whereas it increased to 4--6% in plaque intima. The lipid composition of elastin preparations varied significantly with the extraction procedure. Elastin from plaque intima contained significantly more cholesterol (50--60%) and less triglyceride and phospholipid than elastin of non-plaque intima (30--50% cholesterol). The contents of free and esterified cholesterol were comparable in all preparations. The main phospholipid in all samples was sphingomyelin, which comprised between 50 and 80% of the total phospholipid. Compared with NaOH-purified elastin, the other elastin samples were characterized by an increased phosphatidyl--choline content, while they all contained an almost equal amount of phosphatidylethanolamine. In elastin samples from plaque intima, the polar amino acids were increased, whereas cross-linking amino acids were decreased. The polarity and hydroxyproline content of elastin samples were slightly decreased after treatment with EDTA or dithioerythritol--urea--sodium dodecyl sulfate.", "contents": "Elastin--lipid interaction action in the arterial wall. Part 1. Extraction of elastin from human aortic intima. Elastin was extracted from human aortic plaque and adjacent grossly normal intima by the following methods: (1) 0.1 N NaOH at 100 degrees C, (2) hot NaOH and 0.2 M EDTA, (3) 5 M guanidine--HCl and collagenase, (4) guanidine--collagenase and dithioerythritol--urea--sodium dodecyl sulfate, (5) guanidine--collagenase and EDTA, (6) 10% NaCl and collagenase, and (7) NaCl--collagenase and EDTA. All elastin samples contained small amounts of carbohydrate and hydroxyproline. The lipid content of non-plaque intimal elastin samples was small (2--3%), whereas it increased to 4--6% in plaque intima. The lipid composition of elastin preparations varied significantly with the extraction procedure. Elastin from plaque intima contained significantly more cholesterol (50--60%) and less triglyceride and phospholipid than elastin of non-plaque intima (30--50% cholesterol). The contents of free and esterified cholesterol were comparable in all preparations. The main phospholipid in all samples was sphingomyelin, which comprised between 50 and 80% of the total phospholipid. Compared with NaOH-purified elastin, the other elastin samples were characterized by an increased phosphatidyl--choline content, while they all contained an almost equal amount of phosphatidylethanolamine. In elastin samples from plaque intima, the polar amino acids were increased, whereas cross-linking amino acids were decreased. The polarity and hydroxyproline content of elastin samples were slightly decreased after treatment with EDTA or dithioerythritol--urea--sodium dodecyl sulfate."}
{"id": "PMID:465129", "title": "Uptake and esterification of circulating carnitine by aorta and heart in rabbits in vivo.", "content": "Disappearance of intravenously injected DL-[methyl-14C]carnitine from the bloodstream and its uptake and esterification by heart and aorta were studied in rabbits fed atherogenic or non-atherogenic control diets. The disappearance rate of [14C]carnitine from the bloodstream was approximately 2-fold greater in animals fed the control diet than in those fed the atherogenic diets. No evidence was found for carnitine esterification in the blood. Circulating [14C]carnitine was taken up and esterified in both the heart and aorta of all animals regardless of diet; however, on comparing dpm/mg lipid-free dry weight, uptake of [14C]carnitine and accumulation of [14C]carnitine esters by the heart was greater (6-fold and 12-fold, respectively) than by the aorta. Analysis of defined arterial segments indicated that aortas in animals fed the atherogenic diet contained greater [14C]carnitine activity (4- to 8-fold) and greater acetyl-[14C]carnitine activity (4-fold) when compared to aortas of control animals; uptake of plasma [14C]carnitine and accumulation of acyl-[14C]carnitine compounds by the heart was independent of diet. Butyryl-[14C]carnitine, although not detected in aortas from animals fed the non-atherogenic or atherogenic diet for only 7 weeks, was detected in aortas from animals fed the atherogenic diet 17 weeks. Butyryl-[14C]carnitine was detected in heart tissue regardless of the animals' dietary regime. The increased uptake of circulating [14C]carnitine and its accumulation as both free and esterfied carnitine in atherosclerotic aortas occurred before the development of extensive macroscopic atherosclerotic lesions; this response of the aorta to atherogenic stimuli was not a general tissue response in that the heart did not respond similarly. Since blood carnitine is found predominantly in the plasma fraction, it is likely that these results refect the uptake and metabolism of plasma carnitine in vivo.", "contents": "Uptake and esterification of circulating carnitine by aorta and heart in rabbits in vivo. Disappearance of intravenously injected DL-[methyl-14C]carnitine from the bloodstream and its uptake and esterification by heart and aorta were studied in rabbits fed atherogenic or non-atherogenic control diets. The disappearance rate of [14C]carnitine from the bloodstream was approximately 2-fold greater in animals fed the control diet than in those fed the atherogenic diets. No evidence was found for carnitine esterification in the blood. Circulating [14C]carnitine was taken up and esterified in both the heart and aorta of all animals regardless of diet; however, on comparing dpm/mg lipid-free dry weight, uptake of [14C]carnitine and accumulation of [14C]carnitine esters by the heart was greater (6-fold and 12-fold, respectively) than by the aorta. Analysis of defined arterial segments indicated that aortas in animals fed the atherogenic diet contained greater [14C]carnitine activity (4- to 8-fold) and greater acetyl-[14C]carnitine activity (4-fold) when compared to aortas of control animals; uptake of plasma [14C]carnitine and accumulation of acyl-[14C]carnitine compounds by the heart was independent of diet. Butyryl-[14C]carnitine, although not detected in aortas from animals fed the non-atherogenic or atherogenic diet for only 7 weeks, was detected in aortas from animals fed the atherogenic diet 17 weeks. Butyryl-[14C]carnitine was detected in heart tissue regardless of the animals' dietary regime. The increased uptake of circulating [14C]carnitine and its accumulation as both free and esterfied carnitine in atherosclerotic aortas occurred before the development of extensive macroscopic atherosclerotic lesions; this response of the aorta to atherogenic stimuli was not a general tissue response in that the heart did not respond similarly. Since blood carnitine is found predominantly in the plasma fraction, it is likely that these results refect the uptake and metabolism of plasma carnitine in vivo."}
{"id": "PMID:465133", "title": "Influence of opium availability on addiction rates in Laos.", "content": "Crude rates of opium addiction were determined in 1972-1974 in ten communities of Laos, representing eight different ethnic groups and three provinces. In six rural communities, data were obtained by a house-to-house survey, and in four urban communities by opium den registration. Communities raising opium poppy as a cash crop had highest crude rates of addiction (7.0-9.8 addicts per 100 people). Thos involved in opium commerce had intermediate rates (4.1-5.5). Where neither opium production nor commerce was present, the communities had the lowest rates of addiction (1.8-2.3).", "contents": "Influence of opium availability on addiction rates in Laos. Crude rates of opium addiction were determined in 1972-1974 in ten communities of Laos, representing eight different ethnic groups and three provinces. In six rural communities, data were obtained by a house-to-house survey, and in four urban communities by opium den registration. Communities raising opium poppy as a cash crop had highest crude rates of addiction (7.0-9.8 addicts per 100 people). Thos involved in opium commerce had intermediate rates (4.1-5.5). Where neither opium production nor commerce was present, the communities had the lowest rates of addiction (1.8-2.3)."}
{"id": "PMID:465136", "title": "The primary structure of the alpha subunit of protocatechuate 3,4-dioxygenase. II. Isolation and sequence of overlap peptides and complete sequence.", "content": "The complete primary structure of the alpha subunit of protocatechuate 3,4-dioxygenase has been determined by automated Edman degradation and carboxypeptidase digestionof the intact alpha chain and of peptides derived from trypsin (N.A. Kohlmiller and J.B. Howard (1979) J. Biol. Chem. 254, 7302-7308) and Staphylococcus aureus protease digestion, and from hydroxylamine and dilute acid cleavage. The alpha chain was found to consist of 200 residues in the following sequence from the NH2-terminal end: (formula: see text).", "contents": "The primary structure of the alpha subunit of protocatechuate 3,4-dioxygenase. II. Isolation and sequence of overlap peptides and complete sequence. The complete primary structure of the alpha subunit of protocatechuate 3,4-dioxygenase has been determined by automated Edman degradation and carboxypeptidase digestionof the intact alpha chain and of peptides derived from trypsin (N.A. Kohlmiller and J.B. Howard (1979) J. Biol. Chem. 254, 7302-7308) and Staphylococcus aureus protease digestion, and from hydroxylamine and dilute acid cleavage. The alpha chain was found to consist of 200 residues in the following sequence from the NH2-terminal end: (formula: see text)."}
{"id": "PMID:465138", "title": "Hepatic siderosis in alcoholics.", "content": "In a population of 157 (120 males, 37 females) predominantly British alcoholics with liver disease, the incidence of some degree of hepatic siderosis, as estimated by stainable parenchymal iron, was 57.3%. The incidence of significant siderosis (grades III and IV) was 7%, and was similar for both sexes. In the female alcoholics there was a significant correlation between age and the degree of siderosis (P less than 0.05)--four of the five females with significant siderosis being premenopausal. In the male alcoholics there was a significant inverse relationship between the grams of ethanol consumed per day and the degree of siderosis (P less than 0.05) and a significant correlation between the percentage saturation of iron-binding protein and the degree of siderosis (P less than 0.05). The mean daily iron intake from alcoholic beverages was 1.5 mg; there was no relationship between the amount of iron ingested in the alcohol and the degree of siderosis. In this population of alcoholics the incidence of significant siderosis in both sexes was low.", "contents": "Hepatic siderosis in alcoholics. In a population of 157 (120 males, 37 females) predominantly British alcoholics with liver disease, the incidence of some degree of hepatic siderosis, as estimated by stainable parenchymal iron, was 57.3%. The incidence of significant siderosis (grades III and IV) was 7%, and was similar for both sexes. In the female alcoholics there was a significant correlation between age and the degree of siderosis (P less than 0.05)--four of the five females with significant siderosis being premenopausal. In the male alcoholics there was a significant inverse relationship between the grams of ethanol consumed per day and the degree of siderosis (P less than 0.05) and a significant correlation between the percentage saturation of iron-binding protein and the degree of siderosis (P less than 0.05). The mean daily iron intake from alcoholic beverages was 1.5 mg; there was no relationship between the amount of iron ingested in the alcohol and the degree of siderosis. In this population of alcoholics the incidence of significant siderosis in both sexes was low."}
{"id": "PMID:465141", "title": "[Computed tomography of the heart: anatomical findings in normal subjects (author's transl)].", "content": "Clear images of the chambers of the heart and the vessels can be obtained by rapid scanning with computed tomography, associated with rapid perfusion of a contrast medium. A study in 30 normal subjects demonstrated that it was possible to identify and localize the heart chambers and the large vessels in different planes, and to obtain precise measurements of the various diameters. These results could form the basis for etiological studies of the cardiopathies.", "contents": "[Computed tomography of the heart: anatomical findings in normal subjects (author's transl)]. Clear images of the chambers of the heart and the vessels can be obtained by rapid scanning with computed tomography, associated with rapid perfusion of a contrast medium. A study in 30 normal subjects demonstrated that it was possible to identify and localize the heart chambers and the large vessels in different planes, and to obtain precise measurements of the various diameters. These results could form the basis for etiological studies of the cardiopathies."}
{"id": "PMID:465142", "title": "[Retrograde carotid approach to aortography in neonates and babies: technique and results in 21 cases (author's transl)].", "content": "A new radiological technique is described for opacification of the thoracic aorta in neonates and babies weighing less than 10 kg. The exploration method is described: injection of a contrast medium after retrograde carotid puncture, and increased endothoracic pressure. The results obtained in 21 babies aged from 3 to 157 weeks, and weighing 3.3 to 10 kg are discussed. Radiographic results were excellent in 17 cases, satisfactory in 3 cases, and only moderate in 1 case. The procedure was perfectly well tolerated, and enabled visualization of an arterial canal in 4 cases, stenosis of a Blalock-Taussig anastomosis in 1 case, aortic stenosis in 7 cases (2 tubular hypoptasias, 1 coarctation, 3 segmentary interruptions, 1 recoarctation), coronary-cardiac fistula in 1 case, and a normal aorta in 9 cases. The authors review the advantages, inconveniences, and risks of the other methods of opacification of the thoracic aorta in neonates and babies. They conclude that when right cardiac catheterization cannot give, in babies under 10 kg of weight, a satisfactory radiological assessment of the thoracic aorta in the following clinical conditions: coarctation syndrome, badly tolerated left-right shunt, or possible abnormality of the aortic arch or coronaries, the technique of opacification of the thoracic aorta by retrograde puncture of the common carotid is the best radiological method for establishing a definite diagnosis.", "contents": "[Retrograde carotid approach to aortography in neonates and babies: technique and results in 21 cases (author's transl)]. A new radiological technique is described for opacification of the thoracic aorta in neonates and babies weighing less than 10 kg. The exploration method is described: injection of a contrast medium after retrograde carotid puncture, and increased endothoracic pressure. The results obtained in 21 babies aged from 3 to 157 weeks, and weighing 3.3 to 10 kg are discussed. Radiographic results were excellent in 17 cases, satisfactory in 3 cases, and only moderate in 1 case. The procedure was perfectly well tolerated, and enabled visualization of an arterial canal in 4 cases, stenosis of a Blalock-Taussig anastomosis in 1 case, aortic stenosis in 7 cases (2 tubular hypoptasias, 1 coarctation, 3 segmentary interruptions, 1 recoarctation), coronary-cardiac fistula in 1 case, and a normal aorta in 9 cases. The authors review the advantages, inconveniences, and risks of the other methods of opacification of the thoracic aorta in neonates and babies. They conclude that when right cardiac catheterization cannot give, in babies under 10 kg of weight, a satisfactory radiological assessment of the thoracic aorta in the following clinical conditions: coarctation syndrome, badly tolerated left-right shunt, or possible abnormality of the aortic arch or coronaries, the technique of opacification of the thoracic aorta by retrograde puncture of the common carotid is the best radiological method for establishing a definite diagnosis."}
{"id": "PMID:465143", "title": "[Vascular risk in angiography examinations (author's transl)].", "content": "About an experience of 7,757 investigations and after study of the published series in the literature, it appears the frequency of the accidents following vascular roentgenology has not really changed since ten years. -- 0,5% complications; 0,07% death in aortographies; -- 0,4% complications; 0,09% death in femoral artery catheterization; -- 3% complications; no death in axillary artery catheterization. The authors use the term \"complications\" when a surgical operation is necessary or after effects are present in the evolution. The study of the recent literature proves no significant amelioration in comparison with the last ten years. The accidents are analysed in aortography, in arterial catheterization using femoral or axillary arteries: the latter is the most dangerous. The considerable increment of the number of investigations suppressed the progresses provided by the material improvements. An explanation: in hospitals, it is necessary to train the students for roentgenology.", "contents": "[Vascular risk in angiography examinations (author's transl)]. About an experience of 7,757 investigations and after study of the published series in the literature, it appears the frequency of the accidents following vascular roentgenology has not really changed since ten years. -- 0,5% complications; 0,07% death in aortographies; -- 0,4% complications; 0,09% death in femoral artery catheterization; -- 3% complications; no death in axillary artery catheterization. The authors use the term \"complications\" when a surgical operation is necessary or after effects are present in the evolution. The study of the recent literature proves no significant amelioration in comparison with the last ten years. The accidents are analysed in aortography, in arterial catheterization using femoral or axillary arteries: the latter is the most dangerous. The considerable increment of the number of investigations suppressed the progresses provided by the material improvements. An explanation: in hospitals, it is necessary to train the students for roentgenology."}
{"id": "PMID:465144", "title": "[Radiological aspects of infections due to mycoplasma pneumoniae in children (author's transl)].", "content": "The authors review 69 cases of infections due to mycoplasma pneumoniae in children, including the clinical, biological, and radiological aspects, and compare their results with those in the published literature. Clinical and radiological signs and non-specific, and diagnosis is made exclusively by serological tests.", "contents": "[Radiological aspects of infections due to mycoplasma pneumoniae in children (author's transl)]. The authors review 69 cases of infections due to mycoplasma pneumoniae in children, including the clinical, biological, and radiological aspects, and compare their results with those in the published literature. Clinical and radiological signs and non-specific, and diagnosis is made exclusively by serological tests."}
{"id": "PMID:465145", "title": "[Ultrasonography of the pancreas. Diagnostic problems and findings in cases of solid pancreatic masses (author's transl)].", "content": "By using rigourous examination techniques and interpretive criteria, ultrasonography of the pancreas can, in many cases, confirm the etiological diagnosis of solid pancreatic masses. Factors influencing diagnosis are the volume and shape of the pancreas, the ultrasound structure, the attenuation of ultrasounds, the outline of the pancreas, and the effect on neighbouring structures. The association of signs is often characteristic and can differentiate benign from malignant masses.", "contents": "[Ultrasonography of the pancreas. Diagnostic problems and findings in cases of solid pancreatic masses (author's transl)]. By using rigourous examination techniques and interpretive criteria, ultrasonography of the pancreas can, in many cases, confirm the etiological diagnosis of solid pancreatic masses. Factors influencing diagnosis are the volume and shape of the pancreas, the ultrasound structure, the attenuation of ultrasounds, the outline of the pancreas, and the effect on neighbouring structures. The association of signs is often characteristic and can differentiate benign from malignant masses."}
{"id": "PMID:465146", "title": "[False tumor of the posterior mediastinum in a case of portal hypertension (author's transl)].", "content": "A man of 37 years of age developed portal hypertension which was observed on radiological examination as a mediastinal opacity having the appearance of a tumor. No oesophageal varices were seen on barium meal examination or by endoscopy. A smooth liver was seen during laparoscopy. Pre-operative hemodynamic studies showed that there was no sinus block. Thoracotomy revealed an aneurysm of the azygos veins. Exploratory laparotomy after coelio-mesenteric arteriography confirmed the diagnosis of diffuse nodular hyperplasia of the liver, cirrhosis, and portal hypertension. The findings in 16 cases of mediastinal opacity from portal hypertension reported in the published literature are discussed.", "contents": "[False tumor of the posterior mediastinum in a case of portal hypertension (author's transl)]. A man of 37 years of age developed portal hypertension which was observed on radiological examination as a mediastinal opacity having the appearance of a tumor. No oesophageal varices were seen on barium meal examination or by endoscopy. A smooth liver was seen during laparoscopy. Pre-operative hemodynamic studies showed that there was no sinus block. Thoracotomy revealed an aneurysm of the azygos veins. Exploratory laparotomy after coelio-mesenteric arteriography confirmed the diagnosis of diffuse nodular hyperplasia of the liver, cirrhosis, and portal hypertension. The findings in 16 cases of mediastinal opacity from portal hypertension reported in the published literature are discussed."}
{"id": "PMID:465147", "title": "[Vaginal membrane with hydrocolpos and pyocolpos in children (author's transl)].", "content": "A child aged 4 1/2 years was found to have secondarily infected retained secretions in the vagina from a vaginal membrane. The authors underline the value of intravenous urography coupled with rectal insufflation, which is essential in order to confirm the diagnosis of colpos and to eliminate any associated urinary malformation.", "contents": "[Vaginal membrane with hydrocolpos and pyocolpos in children (author's transl)]. A child aged 4 1/2 years was found to have secondarily infected retained secretions in the vagina from a vaginal membrane. The authors underline the value of intravenous urography coupled with rectal insufflation, which is essential in order to confirm the diagnosis of colpos and to eliminate any associated urinary malformation."}
{"id": "PMID:465149", "title": "[Role of the barrier system of the cerebral membranes in subarachnoid hemorrhage].", "content": "On the basis of literary and his own data the author distinguishes 3 stages of subarachnoidal hemorrhage, each having its own specific pathophysiological and pathomorphological features. The extracerebral barrier system can be divided into 3 structural and functional groups, such as: a) barriers between the cerebrospinal liquor and the blood; b) barriers between the liquor and the border tissues, and c) histohematic barriers. In the conditions of subarachnoidal hemorrhage accompanied with development of arterial spasm one observes disturbances of the liquor microcirculation because of blood clotting in the liquor and sticking of the blood clots in various parts of the subarachnoidal space, as well as changes in the ultrastructure of the morphological substrate of the extracerebral barrier system. All this, in the author's opinion, leads to damaging the innervation apparatus elements and smooth muscle cells of the cerebral arteries and arterioles; promotes the development of cerebral edema, etc. The data obtained make it possible to evaluate the role of the extracerebral barrier system of the meninges in cases of subarachnoidal hemorrhage with accompanying arterial spasm.", "contents": "[Role of the barrier system of the cerebral membranes in subarachnoid hemorrhage]. On the basis of literary and his own data the author distinguishes 3 stages of subarachnoidal hemorrhage, each having its own specific pathophysiological and pathomorphological features. The extracerebral barrier system can be divided into 3 structural and functional groups, such as: a) barriers between the cerebrospinal liquor and the blood; b) barriers between the liquor and the border tissues, and c) histohematic barriers. In the conditions of subarachnoidal hemorrhage accompanied with development of arterial spasm one observes disturbances of the liquor microcirculation because of blood clotting in the liquor and sticking of the blood clots in various parts of the subarachnoidal space, as well as changes in the ultrastructure of the morphological substrate of the extracerebral barrier system. All this, in the author's opinion, leads to damaging the innervation apparatus elements and smooth muscle cells of the cerebral arteries and arterioles; promotes the development of cerebral edema, etc. The data obtained make it possible to evaluate the role of the extracerebral barrier system of the meninges in cases of subarachnoidal hemorrhage with accompanying arterial spasm."}
{"id": "PMID:465150", "title": "[Ultrastructural characteristics of the intracerebral components of the blood-brain barrier].", "content": "Electron microscopic studies of interactions between the cerebral tissue, the basal membrane of intracerebral vessels, and the limiting superficial membrane have been carried out. It has been demonstrated that highly specialized contacts of the compact and gap type are maintained constantly between the membranes of the neighboring astrocyte spicules around the vessels and under the limiting membrane. Such contacts are also encountered in the intercellular space in the whole mass of the brain. It is suggested that this specialized system and the basal membrane are of importance for regulating the functioning of the hematoencephalic barrier. It is noted that there exists a united structured system at the border between the cerebral tissue, cerebrospinal fluid, and blood, the participates in the pathogenetic and homeostatic mechanisms.", "contents": "[Ultrastructural characteristics of the intracerebral components of the blood-brain barrier]. Electron microscopic studies of interactions between the cerebral tissue, the basal membrane of intracerebral vessels, and the limiting superficial membrane have been carried out. It has been demonstrated that highly specialized contacts of the compact and gap type are maintained constantly between the membranes of the neighboring astrocyte spicules around the vessels and under the limiting membrane. Such contacts are also encountered in the intercellular space in the whole mass of the brain. It is suggested that this specialized system and the basal membrane are of importance for regulating the functioning of the hematoencephalic barrier. It is noted that there exists a united structured system at the border between the cerebral tissue, cerebrospinal fluid, and blood, the participates in the pathogenetic and homeostatic mechanisms."}
{"id": "PMID:465151", "title": "[Microglia-type cells in normal and pathologic human embryonic brains].", "content": "The brains of 7--12 week embryos, developing in normal and mentally ill females (normal--14, schizophrenia--12, other mental disorders--10) were studied by means of electron microscopy. It was established that the cells of the microglia type may be encountered in the brain of embryos beginning from 7 weeks. In the brain of embryos from normal females these cells had mainly a round or oval form (globose microglia). Axons were encountered relatively rarely. Some of the cells had protrusions of the pseudopodia-like type. In the brain of embryos from mentally ill females the cells of the microglia type have diverse, sometimes sticklike forms; they form multiple thin axons, actively fagocyte. The ultrastructure in such conditions was not destructed. These changes are considered to be the result of an increased activity of microglial cells under the influence of factors of the pathological process.", "contents": "[Microglia-type cells in normal and pathologic human embryonic brains]. The brains of 7--12 week embryos, developing in normal and mentally ill females (normal--14, schizophrenia--12, other mental disorders--10) were studied by means of electron microscopy. It was established that the cells of the microglia type may be encountered in the brain of embryos beginning from 7 weeks. In the brain of embryos from normal females these cells had mainly a round or oval form (globose microglia). Axons were encountered relatively rarely. Some of the cells had protrusions of the pseudopodia-like type. In the brain of embryos from mentally ill females the cells of the microglia type have diverse, sometimes sticklike forms; they form multiple thin axons, actively fagocyte. The ultrastructure in such conditions was not destructed. These changes are considered to be the result of an increased activity of microglial cells under the influence of factors of the pathological process."}
{"id": "PMID:465152", "title": "[Morphologic changes in the brain after sustaining asphyxia during labor (pathogenesis of mental retardation)].", "content": "An analysis was accomplished of pathomorphological changes in the brains of 10 newborns, who died of intranatal asphyxia and of 1 adult brain with mental retardation, due to asphyxia during delivery. The dynamics of hypoxic changes of an irreversible character in the brain of the newborn and the existence of chronic hypoxic encephalopathy with pronounced cellular devastation in the late period of asphyxia indicate common genesis of the pathological process in the brain, conditioning its progression. The role of intranatal asphyxia in the pathogenesis of changes in postnatal development of neuropsychic activity is discussed.", "contents": "[Morphologic changes in the brain after sustaining asphyxia during labor (pathogenesis of mental retardation)]. An analysis was accomplished of pathomorphological changes in the brains of 10 newborns, who died of intranatal asphyxia and of 1 adult brain with mental retardation, due to asphyxia during delivery. The dynamics of hypoxic changes of an irreversible character in the brain of the newborn and the existence of chronic hypoxic encephalopathy with pronounced cellular devastation in the late period of asphyxia indicate common genesis of the pathological process in the brain, conditioning its progression. The role of intranatal asphyxia in the pathogenesis of changes in postnatal development of neuropsychic activity is discussed."}
{"id": "PMID:465153", "title": "[Effect of tissue hypoxia on the structure of cerebral cortex neurons (light--electron microscopic study)].", "content": "An examination of the cerebral cortex of rats exposed to sublethal doses of potassium cyanide has revealed changes in the neurons characteristic of the developing histotoxic hypoxia. The neurons of the large hemisphere cortex showed a pronounced hydropic reaction in their cytoplasm with prevalent involvement of the mitochondria. This form of the pathology was observed irrespectively of the duration of the animals live after the poisoning. In rats which survived for a longer time there appeared hyperchromic neurons the number of which gradually increased. This fact was interpreted as addition of the hypoxic state of the neurons to the cyanide intoxication: this was confirmed by development of signs characteristic of hypoxic hypoxia at this stage. The degree of the changes in the mitochondria was found to correlate with the depth of the impairment of vitally important neuron structures. The data obtained show that the primary morphological changes in the neurons develop in their cytomembranes. The degree of the impairment of the mitochondrial membranes can serve as a criterion of reversibility or irreversibility of the pathological process in the nerve cells.", "contents": "[Effect of tissue hypoxia on the structure of cerebral cortex neurons (light--electron microscopic study)]. An examination of the cerebral cortex of rats exposed to sublethal doses of potassium cyanide has revealed changes in the neurons characteristic of the developing histotoxic hypoxia. The neurons of the large hemisphere cortex showed a pronounced hydropic reaction in their cytoplasm with prevalent involvement of the mitochondria. This form of the pathology was observed irrespectively of the duration of the animals live after the poisoning. In rats which survived for a longer time there appeared hyperchromic neurons the number of which gradually increased. This fact was interpreted as addition of the hypoxic state of the neurons to the cyanide intoxication: this was confirmed by development of signs characteristic of hypoxic hypoxia at this stage. The degree of the changes in the mitochondria was found to correlate with the depth of the impairment of vitally important neuron structures. The data obtained show that the primary morphological changes in the neurons develop in their cytomembranes. The degree of the impairment of the mitochondrial membranes can serve as a criterion of reversibility or irreversibility of the pathological process in the nerve cells."}
{"id": "PMID:465154", "title": "[Changes in the state of the blood-brain barrier in rats following experimental neurosensitization].", "content": "The accumulation and distribution of phosphorus radioisotope was studied in different brain areas of rats. These rats were desensitized by antigens of a whole homologous brain and its separate structures. A certain general tendency towards an accumulation of the brain phosphorus was detected after neurosensitization. Besides, in sensitization by separate structures (brain cortex, cerebellum) the most pronounced changes were in the hematoencephalic barrier (HEB) of the corresponding brain areas are less pronounced in other structures. The paper contains data concerning the function of HEB in animals immunized by the medulla oblongata. The found changes in the barrier mechanisms of the HEB in neurosensitization may be one of the pathogenetical mechanisms of certain diseases accompanied by neuroautoimmune processes.", "contents": "[Changes in the state of the blood-brain barrier in rats following experimental neurosensitization]. The accumulation and distribution of phosphorus radioisotope was studied in different brain areas of rats. These rats were desensitized by antigens of a whole homologous brain and its separate structures. A certain general tendency towards an accumulation of the brain phosphorus was detected after neurosensitization. Besides, in sensitization by separate structures (brain cortex, cerebellum) the most pronounced changes were in the hematoencephalic barrier (HEB) of the corresponding brain areas are less pronounced in other structures. The paper contains data concerning the function of HEB in animals immunized by the medulla oblongata. The found changes in the barrier mechanisms of the HEB in neurosensitization may be one of the pathogenetical mechanisms of certain diseases accompanied by neuroautoimmune processes."}
{"id": "PMID:465155", "title": "[Heterogeneity of neurocytes of different brain regions to repeated superhigh-frequency irradiation].", "content": "The study was based on the analysis of morphological changes in the brain of 45 rats exposed for 10 days to ultrahigh frequency field of moderate gravity. Detailed studies of the state of the basophilic substance and the volume of the nuclei in the course of the neurocyte swelling were undertaken. The response of the neurocytes of various brain divisions was found to be heterogenous. The most pronounced were dystrophic changes in the sincipital area of the cortex. A lack of correlation between the character of the nuclear swelling and the degree of chromatolysis in the thalamic neurocytes was noted.", "contents": "[Heterogeneity of neurocytes of different brain regions to repeated superhigh-frequency irradiation]. The study was based on the analysis of morphological changes in the brain of 45 rats exposed for 10 days to ultrahigh frequency field of moderate gravity. Detailed studies of the state of the basophilic substance and the volume of the nuclei in the course of the neurocyte swelling were undertaken. The response of the neurocytes of various brain divisions was found to be heterogenous. The most pronounced were dystrophic changes in the sincipital area of the cortex. A lack of correlation between the character of the nuclear swelling and the degree of chromatolysis in the thalamic neurocytes was noted."}
{"id": "PMID:465156", "title": "[Structure of the viscerocutaneous sensitive syndrome (pathogenesis of Zakhar'in--Head zones)].", "content": "On the basis of literature analysis, clinical observations, and examinations of the spinal cord ganglia of man (autopsy material) and experimental animals (dogs) performed with the use of a set of naurohistological and cytochemical methods an original explanation of the pathogenesis of the Head zones is suggested. The principal link in the formation of the viscero-cutaneous and viscero-sclerotomic sensation syndrome are transitory presynaptic terminals formed by the axons of the vegetative sensor neurons (cells of Dogel type 2) on the surface of the somatic receptor neurons innervating the skin and the sclerotomic tissues, and responding to temperature and pain. Within the zone of the innervation by the dendrites of these somatic receptor neurons (cutaneous and sclerotomic) a reflected viscero-sensory syndrome is formed. The formation of these viscero-cutaneous and viscero-sclerotomic syndromes proceeds in 4 steps.", "contents": "[Structure of the viscerocutaneous sensitive syndrome (pathogenesis of Zakhar'in--Head zones)]. On the basis of literature analysis, clinical observations, and examinations of the spinal cord ganglia of man (autopsy material) and experimental animals (dogs) performed with the use of a set of naurohistological and cytochemical methods an original explanation of the pathogenesis of the Head zones is suggested. The principal link in the formation of the viscero-cutaneous and viscero-sclerotomic sensation syndrome are transitory presynaptic terminals formed by the axons of the vegetative sensor neurons (cells of Dogel type 2) on the surface of the somatic receptor neurons innervating the skin and the sclerotomic tissues, and responding to temperature and pain. Within the zone of the innervation by the dendrites of these somatic receptor neurons (cutaneous and sclerotomic) a reflected viscero-sensory syndrome is formed. The formation of these viscero-cutaneous and viscero-sclerotomic syndromes proceeds in 4 steps."}
{"id": "PMID:465158", "title": "[Thermopulsation rhythms and their importance to the physiology of thermoregulation and clinical practice].", "content": "Application of thermopulsation as a method of action on the human temperature analyzer for investigative, diagnostic and therapeutic purposes is substantiated on the basis of previous works of the author and his coworkers. New thermopulsation techniques are described and the results of their use are compared. Ideas of some specific mechanisms of thermoregulation and its clinical application are formulated in view of the fact that thermopulsation has proved highly efficacious for respiratory rhythms.", "contents": "[Thermopulsation rhythms and their importance to the physiology of thermoregulation and clinical practice]. Application of thermopulsation as a method of action on the human temperature analyzer for investigative, diagnostic and therapeutic purposes is substantiated on the basis of previous works of the author and his coworkers. New thermopulsation techniques are described and the results of their use are compared. Ideas of some specific mechanisms of thermoregulation and its clinical application are formulated in view of the fact that thermopulsation has proved highly efficacious for respiratory rhythms."}
{"id": "PMID:465159", "title": "[Syndrome of aspontaneity in frontal lobe tumors].", "content": "The syndrome of aspontaneousity in tumors of the frontal lobes is expressed in a sharp decrease of initiative, spontaneous volitions, crude changes in the emotional sphere and peculiar disorders of thinking. In a \"clear\" form, i. e. without changed consciousness, the syndrome proceeds with signs of increased distractability and a combination of distrubances of wakefulness with rapid fatigueability. The first variant is more characteristic of bilateral intracerebral tumors, located on the level of the callosum sorpus. The second--for large unilateral tumors, exerting influence on the opposite frontal lobe and frequently on the diencephalic area.", "contents": "[Syndrome of aspontaneity in frontal lobe tumors]. The syndrome of aspontaneousity in tumors of the frontal lobes is expressed in a sharp decrease of initiative, spontaneous volitions, crude changes in the emotional sphere and peculiar disorders of thinking. In a \"clear\" form, i. e. without changed consciousness, the syndrome proceeds with signs of increased distractability and a combination of distrubances of wakefulness with rapid fatigueability. The first variant is more characteristic of bilateral intracerebral tumors, located on the level of the callosum sorpus. The second--for large unilateral tumors, exerting influence on the opposite frontal lobe and frequently on the diencephalic area."}
{"id": "PMID:465160", "title": "[Muscle spasms in patients with amyotrophic lateral sclerosis (clinico-electromyographnic analysis)].", "content": "The diagnostic importance and electromyographic characteristics of the muscular spasms were studied in patients with lateral amyotrophic sclerosis at various stages of the disease. It has been shown that the muscular spasms are a typical symptom of this disease, and may be an early manifestation of the latter. The clinical and electromyographic characteristics of the spasms depend on the depth of the pathological process. Of importance in the origin of the muscular spasms in the lateral amyotrophic sclerosis is involvement of many levels of the cortico-muscular path, however, distrubances of the intraspinal mechanisms are, probably, the leading cause.", "contents": "[Muscle spasms in patients with amyotrophic lateral sclerosis (clinico-electromyographnic analysis)]. The diagnostic importance and electromyographic characteristics of the muscular spasms were studied in patients with lateral amyotrophic sclerosis at various stages of the disease. It has been shown that the muscular spasms are a typical symptom of this disease, and may be an early manifestation of the latter. The clinical and electromyographic characteristics of the spasms depend on the depth of the pathological process. Of importance in the origin of the muscular spasms in the lateral amyotrophic sclerosis is involvement of many levels of the cortico-muscular path, however, distrubances of the intraspinal mechanisms are, probably, the leading cause."}
{"id": "PMID:465161", "title": "[Neurologic manifestations of periodic disease].", "content": "Results of comprehensive neurological examinations of 4 patients suffering from periodic disease have shown that the latter may be not only a disease entity, but also a syndrome characterizing some organic affections of the brain. The abdominal and other crises of the periodic disease included cerebrovascular disturbances which manifested hemipareses, syncopes, and migraine-like cephalgia. EEG and REG examinations have revealed dysfunction of the mesodiencephalic structures which, probably, underlies the pathogenesis of the periodic disease. The knowledge of the formerly almost unexplored neurological manifestations of the periodic disease will contribute to improvement of the therapy of this rare ailment.", "contents": "[Neurologic manifestations of periodic disease]. Results of comprehensive neurological examinations of 4 patients suffering from periodic disease have shown that the latter may be not only a disease entity, but also a syndrome characterizing some organic affections of the brain. The abdominal and other crises of the periodic disease included cerebrovascular disturbances which manifested hemipareses, syncopes, and migraine-like cephalgia. EEG and REG examinations have revealed dysfunction of the mesodiencephalic structures which, probably, underlies the pathogenesis of the periodic disease. The knowledge of the formerly almost unexplored neurological manifestations of the periodic disease will contribute to improvement of the therapy of this rare ailment."}
{"id": "PMID:465162", "title": "[Experience with X-ray study of the skull structure of schizophrenic patients and their relatives].", "content": "Anthropological signs of the skull were studied in 431 schizophrenic patients, 99 parents (64 mothers and 35 fathers), and in 1371 individuals in the control group. By means of a comparitive polyvectoral statistical analysis a significant accumulation of abnormal and rare variants of the skull structure was established among the schizophrenic patients and their parents which significantly exceeds the frequency in a mentally healthy population (P less than 0,01--P less than 0,001).", "contents": "[Experience with X-ray study of the skull structure of schizophrenic patients and their relatives]. Anthropological signs of the skull were studied in 431 schizophrenic patients, 99 parents (64 mothers and 35 fathers), and in 1371 individuals in the control group. By means of a comparitive polyvectoral statistical analysis a significant accumulation of abnormal and rare variants of the skull structure was established among the schizophrenic patients and their parents which significantly exceeds the frequency in a mentally healthy population (P less than 0,01--P less than 0,001)."}
{"id": "PMID:465163", "title": "[Pathomorphology of the brains of schizophrenic patients treated with psychotropic drugs (concerning drug pathomorphosis)].", "content": "Histological methods demonstrated that the use of psychopharmacological drugs in schizophrenic patients does not remove the characteristic neuron pathology (shrinkage, atrophy, lipofuscin dystrophy, cytolysis), but increases the metabolic processes in the intact nervous cells (an increase in the volume and basophilia in the cytoplasma, an enlargement of nuclei and nucleoli) and the glia activity (an increase in the amount of perineuronal satellites, general glia and the glioneuronal index). It is believed that the treatment does not remove the organic brain defect, but stimulates the metabolic processes in the CNS.", "contents": "[Pathomorphology of the brains of schizophrenic patients treated with psychotropic drugs (concerning drug pathomorphosis)]. Histological methods demonstrated that the use of psychopharmacological drugs in schizophrenic patients does not remove the characteristic neuron pathology (shrinkage, atrophy, lipofuscin dystrophy, cytolysis), but increases the metabolic processes in the intact nervous cells (an increase in the volume and basophilia in the cytoplasma, an enlargement of nuclei and nucleoli) and the glia activity (an increase in the amount of perineuronal satellites, general glia and the glioneuronal index). It is believed that the treatment does not remove the organic brain defect, but stimulates the metabolic processes in the CNS."}
{"id": "PMID:465164", "title": "[Pathohistologic and biochemical changes in the brain in atherosclerotic dementia].", "content": "The data obtained from studies of 48 postmortem cases of atherosclerotic dementia with the use of histological and biochemical methods demonstrated a prevalence of ischemic changes in the nervous cells, cells-shadows, demyelinization, fragmentation of nervous filaments, hyperplasia, hypertrophy of astrocytes, a swelling of oligodendroglia compared to other changes. A drop in the amount of lecithin in the brain is the highest, while that in kefaline fractions is the lowest. A drop in the amount of sphyngomyelin occupies a middle place.", "contents": "[Pathohistologic and biochemical changes in the brain in atherosclerotic dementia]. The data obtained from studies of 48 postmortem cases of atherosclerotic dementia with the use of histological and biochemical methods demonstrated a prevalence of ischemic changes in the nervous cells, cells-shadows, demyelinization, fragmentation of nervous filaments, hyperplasia, hypertrophy of astrocytes, a swelling of oligodendroglia compared to other changes. A drop in the amount of lecithin in the brain is the highest, while that in kefaline fractions is the lowest. A drop in the amount of sphyngomyelin occupies a middle place."}
{"id": "PMID:465165", "title": "[Several diagnostic errors in cerebral atherosclerosis (clinico-anatomic study)].", "content": "A clinicoanatomical study was conducted in 218 cases of atherosclerotic dementia. 12 cases (5,5% of the total amount) showed diagnostic errors. Atherosclerotic dementia with Alzhemier-like symptomatology during life was considered to be Alzheimers disease, while Alzheimers disease, complicated by cerebral atherosclerosis as atherosclerotic dementia. Some objective and subjective factors of diagnostic errors were established. Late detection of such patients is considered as one of the risk factors of diagnostic errors during life.", "contents": "[Several diagnostic errors in cerebral atherosclerosis (clinico-anatomic study)]. A clinicoanatomical study was conducted in 218 cases of atherosclerotic dementia. 12 cases (5,5% of the total amount) showed diagnostic errors. Atherosclerotic dementia with Alzhemier-like symptomatology during life was considered to be Alzheimers disease, while Alzheimers disease, complicated by cerebral atherosclerosis as atherosclerotic dementia. Some objective and subjective factors of diagnostic errors were established. Late detection of such patients is considered as one of the risk factors of diagnostic errors during life."}
{"id": "PMID:465168", "title": "[Radiological and manometric study of gastroesophageal reflux in normal infants].", "content": "The incidence of the gastroesophageal reflux was studied in 39 healthy infants with ages between 2 and 24 months. X-ray studies of gastroesophageal passage and esophageal manometry were practiced in all cases. Only 3 infants showed radiological reflux. The pressure in the gastroesophageal sphincter was normal in all babies and the highest pressures were reached less--than 6 months--old infants. Older infants showed lesser pressures. The same was true for the length of the gastroesophageal sphincter. The factors involved in the genesis of the gastroesophageal reflux are discussed.", "contents": "[Radiological and manometric study of gastroesophageal reflux in normal infants]. The incidence of the gastroesophageal reflux was studied in 39 healthy infants with ages between 2 and 24 months. X-ray studies of gastroesophageal passage and esophageal manometry were practiced in all cases. Only 3 infants showed radiological reflux. The pressure in the gastroesophageal sphincter was normal in all babies and the highest pressures were reached less--than 6 months--old infants. Older infants showed lesser pressures. The same was true for the length of the gastroesophageal sphincter. The factors involved in the genesis of the gastroesophageal reflux are discussed."}
{"id": "PMID:465169", "title": "[Electrophysiologic studies in Guillain-Barr\u00e9 syndrome].", "content": "A preliminary investigation into electrodiagnostic methods in healthy children and in children with the Guillain-Barr\u00e9 syndrome (polyradiculoneuritis) was carried out in order to assess the validity of these procedures in the management of the disease. Studies of the strength-duration curves, the rheobase, the chronaxyie, the accommodation index curves, motor nerve conduction and electromyography were made in 30 healthy children and in 15 children suffering from polyradiculoneuritis. The strength-duration curves plotted on the rectus femoris muscles of the 15 sick children showed a steeply rising hyperbola and no response was elicited at the shorter pulse durations (less than 0.3 ms). The chronaxyie was long in very clinically involved muscle. The accommodation index curve showed a notably decreased slope in the left gastrocnemius muscles of the 15 patients. Conduction velocity of the anterior tibial nerve was either absent or low in all the patients during the first month of the disease. Electromyographic records showed disappearance of the interference pattern on maximal contraction of the diseased muscles; only a few isolated motor units could be recorded in the first month of the disease. The duration and amplitude of these units was markedly reduced, there was an increase in infrequency of polyphasic potentials and the presence of fibrillation potentials was noted in some cases. Serial electrophysiological studies should therefore be of interest and of value in the management of polyradiculoneuritis.", "contents": "[Electrophysiologic studies in Guillain-Barr\u00e9 syndrome]. A preliminary investigation into electrodiagnostic methods in healthy children and in children with the Guillain-Barr\u00e9 syndrome (polyradiculoneuritis) was carried out in order to assess the validity of these procedures in the management of the disease. Studies of the strength-duration curves, the rheobase, the chronaxyie, the accommodation index curves, motor nerve conduction and electromyography were made in 30 healthy children and in 15 children suffering from polyradiculoneuritis. The strength-duration curves plotted on the rectus femoris muscles of the 15 sick children showed a steeply rising hyperbola and no response was elicited at the shorter pulse durations (less than 0.3 ms). The chronaxyie was long in very clinically involved muscle. The accommodation index curve showed a notably decreased slope in the left gastrocnemius muscles of the 15 patients. Conduction velocity of the anterior tibial nerve was either absent or low in all the patients during the first month of the disease. Electromyographic records showed disappearance of the interference pattern on maximal contraction of the diseased muscles; only a few isolated motor units could be recorded in the first month of the disease. The duration and amplitude of these units was markedly reduced, there was an increase in infrequency of polyphasic potentials and the presence of fibrillation potentials was noted in some cases. Serial electrophysiological studies should therefore be of interest and of value in the management of polyradiculoneuritis."}
{"id": "PMID:465170", "title": "[Balance technic in the syndrome of infantile diarrhea].", "content": "Ninety-six infants were studied with water balance technique at the Infant Unit of Hospital Roberto del R\u00edo, Santiago de Chile. Sixty-eight with acute diarrhoea and twenty-eight with protracted diarrhoea associated to carbohydrate intolerance. Total water losses in most of them were in the range of 150 to 225 ml/kg day. Stool loses were in the range of 25 to 75 gm/kg/day, with humidity percentage varing from 97.4 to 92.5%. In patients with acute diarrhoea, stool losses and humidity percentage showed slight decreasing tendency in the following balances. By contrast, those with protracted diarrhoea showed a definite decreasing tendency in both parameters. Most infants showed a urinary output in the range of 20 to 80 ml/kg/day. Ten percent showed oliguria. Polyuria was observed occasionally. Insensible water losses were in the range of 50 to 80 ml/kg/day. Most patients showed weight gain of 0.1 to 5.0% in the first balance. Stool acidity and presence of reductant substances (tested by positivity of Fehling reaction) were found in 24% of infants with acute diarrhoea in the first balance. Sodium concentration in stools varied from 40 to 70 mEq/l; and potassium varied between 20 and 40 mEq/l. Bacterial pathogens were isolated in 64% of patients with acute diarrhoea; most of them E. coli enteropathogenic.", "contents": "[Balance technic in the syndrome of infantile diarrhea]. Ninety-six infants were studied with water balance technique at the Infant Unit of Hospital Roberto del R\u00edo, Santiago de Chile. Sixty-eight with acute diarrhoea and twenty-eight with protracted diarrhoea associated to carbohydrate intolerance. Total water losses in most of them were in the range of 150 to 225 ml/kg day. Stool loses were in the range of 25 to 75 gm/kg/day, with humidity percentage varing from 97.4 to 92.5%. In patients with acute diarrhoea, stool losses and humidity percentage showed slight decreasing tendency in the following balances. By contrast, those with protracted diarrhoea showed a definite decreasing tendency in both parameters. Most infants showed a urinary output in the range of 20 to 80 ml/kg/day. Ten percent showed oliguria. Polyuria was observed occasionally. Insensible water losses were in the range of 50 to 80 ml/kg/day. Most patients showed weight gain of 0.1 to 5.0% in the first balance. Stool acidity and presence of reductant substances (tested by positivity of Fehling reaction) were found in 24% of infants with acute diarrhoea in the first balance. Sodium concentration in stools varied from 40 to 70 mEq/l; and potassium varied between 20 and 40 mEq/l. Bacterial pathogens were isolated in 64% of patients with acute diarrhoea; most of them E. coli enteropathogenic."}
{"id": "PMID:465171", "title": "[Renal function in normal and malnourished children given different doses of vasopressin in continuous infusion].", "content": "The effects of vasopressin administered by continuous infusion (0.75 and 0.5 mU/m2/minutes) was studied in two groups of three normal and two groups of 5 and 8 malnourished children given 0.5 and 0.3 mU/m2/minute. The following parameters were analyzed: urine volume, osmolality, water reabsorption, PAH, urea and inulin clearances, Na and K urinary excretion. Malnourished children had a urine volume 3 to 5 times higher than the normal groups. Vasopressin increased urine volume initially, but a mild antidiuretic effect followed in the normal groups. In malnourished children with a high CH2O, antidiuresis showed quite important figures with vasopressin. A transient fall in PAH and inulin clearances was observed with vasopressin in both malnourished groups with a mild drop in the normal group. Natriuresis with a higher % of the filtered sodium excretion was observed in the malnourished groups and in normal children with 0.5 mU of vasopressin. These results show that vasopressin had similar effects, but at a different level in the normal and malnourished children that we studied.", "contents": "[Renal function in normal and malnourished children given different doses of vasopressin in continuous infusion]. The effects of vasopressin administered by continuous infusion (0.75 and 0.5 mU/m2/minutes) was studied in two groups of three normal and two groups of 5 and 8 malnourished children given 0.5 and 0.3 mU/m2/minute. The following parameters were analyzed: urine volume, osmolality, water reabsorption, PAH, urea and inulin clearances, Na and K urinary excretion. Malnourished children had a urine volume 3 to 5 times higher than the normal groups. Vasopressin increased urine volume initially, but a mild antidiuretic effect followed in the normal groups. In malnourished children with a high CH2O, antidiuresis showed quite important figures with vasopressin. A transient fall in PAH and inulin clearances was observed with vasopressin in both malnourished groups with a mild drop in the normal group. Natriuresis with a higher % of the filtered sodium excretion was observed in the malnourished groups and in normal children with 0.5 mU of vasopressin. These results show that vasopressin had similar effects, but at a different level in the normal and malnourished children that we studied."}
{"id": "PMID:465172", "title": "[Hepatitis A antibodies in children].", "content": "With the finding of HBV and HAV, it is possible at the present time to recognize according to its etiology three types of viral hepatitis: type A, Type B and type non-A-non-B. In this paper we have proved that a low socioeconomic status plays a very important role in the spreading of this disease. In a community with a low socioeconomic status, of forty children attending the fourth grade of a primary school, 97 percent were found to have anti-HA: while in a similar group of children with a higher socioeconomic background, we found only 40 per cent of positive cases. A positive sero convertion to anti-HA was found in 90 per cent of the 73 children with acute hepatitis; the remaining 10 per cent were non-A-non-B. A group of 61 children admitted to the L. Calvo Mackenna Children's Hospital with acute or chronic non hepatic disease were studied for anti-HA. We found 77% positive cases in the group of infants under 4 months old; in older infants, a low incidence of anti-HA was observed, but a notorious increase of positive cases was seen after the age of two years, reaching 100 percent of positive cases in children above four years of age. In ten patients studied with prolonged hepatitis, five of them could be possibly classified as having a non-A-non-B hepatitis.", "contents": "[Hepatitis A antibodies in children]. With the finding of HBV and HAV, it is possible at the present time to recognize according to its etiology three types of viral hepatitis: type A, Type B and type non-A-non-B. In this paper we have proved that a low socioeconomic status plays a very important role in the spreading of this disease. In a community with a low socioeconomic status, of forty children attending the fourth grade of a primary school, 97 percent were found to have anti-HA: while in a similar group of children with a higher socioeconomic background, we found only 40 per cent of positive cases. A positive sero convertion to anti-HA was found in 90 per cent of the 73 children with acute hepatitis; the remaining 10 per cent were non-A-non-B. A group of 61 children admitted to the L. Calvo Mackenna Children's Hospital with acute or chronic non hepatic disease were studied for anti-HA. We found 77% positive cases in the group of infants under 4 months old; in older infants, a low incidence of anti-HA was observed, but a notorious increase of positive cases was seen after the age of two years, reaching 100 percent of positive cases in children above four years of age. In ten patients studied with prolonged hepatitis, five of them could be possibly classified as having a non-A-non-B hepatitis."}
{"id": "PMID:465173", "title": "[Maternal lactation. I. Resistance of maternal milk to bacterial contamination].", "content": "Bacteriologic studies in hands, nipples and milk in 10 wet-nurses were done in order to establish regulation for the recollection of breast milk to be used in low -birth-weight infants. The same studies were done in the hands of 10 nurse-aids, that help in the recollection of breast milk. The results depicts that the breast milk has bacteriostatic and bactericidal properties enough to protect against contamination by germs of the mother's bacterial flora. It does not have the same property against germs that are not of their own flora: those of the nurse-aids's hands. The protection is such that is possible to maintain the breast milk at environmental temperature of 4 degrees C, for 72 hours, without having the developing of germs not isolated previously, and even with the disappearance of some strains present in the initial cultures.", "contents": "[Maternal lactation. I. Resistance of maternal milk to bacterial contamination]. Bacteriologic studies in hands, nipples and milk in 10 wet-nurses were done in order to establish regulation for the recollection of breast milk to be used in low -birth-weight infants. The same studies were done in the hands of 10 nurse-aids, that help in the recollection of breast milk. The results depicts that the breast milk has bacteriostatic and bactericidal properties enough to protect against contamination by germs of the mother's bacterial flora. It does not have the same property against germs that are not of their own flora: those of the nurse-aids's hands. The protection is such that is possible to maintain the breast milk at environmental temperature of 4 degrees C, for 72 hours, without having the developing of germs not isolated previously, and even with the disappearance of some strains present in the initial cultures."}
{"id": "PMID:465175", "title": "[Evolutionary study of 81 children with celiac disease].", "content": "A review of 81 children with celiac disease over a period of 5 years is presented. These cases were seen at the Gastroenterological Unit of the Hospital de Ni\u0144os Roberto del R\u00edo, during years 1970 through 1972. 62% of the patients (50) were placed under control of their disease, the rest were studied and diagnosed only for other centers. The short term evolution was good in 48 children (96%), while the medium and long term evolution, 2 to 5 years, was good only in 56% of the patients. This evolution was in direct relationship to the fulfillment of the dietary indications. The therapeutic test was positive among the studied group. A delay was observed in 80% of the cases that lasted from 1 to 6 months. The clinical and laboratory features of the group are analyzed, and the working method is described.", "contents": "[Evolutionary study of 81 children with celiac disease]. A review of 81 children with celiac disease over a period of 5 years is presented. These cases were seen at the Gastroenterological Unit of the Hospital de Ni\u0144os Roberto del R\u00edo, during years 1970 through 1972. 62% of the patients (50) were placed under control of their disease, the rest were studied and diagnosed only for other centers. The short term evolution was good in 48 children (96%), while the medium and long term evolution, 2 to 5 years, was good only in 56% of the patients. This evolution was in direct relationship to the fulfillment of the dietary indications. The therapeutic test was positive among the studied group. A delay was observed in 80% of the cases that lasted from 1 to 6 months. The clinical and laboratory features of the group are analyzed, and the working method is described."}
{"id": "PMID:465174", "title": "[Maternal lactation. II. Intestinal colonization in breast fed newborn infants].", "content": "Twenty-three low birth weight infants were studied to establish the role that breast milk plays in the intestinal colonization of the preterm infant, and in the control of epidemic diarrhea due to enteropathogenic E. coli, in nurseries. Twelve of these case were fed breast milk in bottles, and eleven with \"humanized\" powder cow's milk (NAN M.R.). Bacteriologic studies of feces were performed daily during the first 18 days of life. In 5 cases given breast milk, and in 8 receiving cow's milk, Staphylococcus aureus was isolated. Enteropathogenic E. coli was found in 3 cases with breast milk and in 6 with cow's milk. The finding of other non-pathogenic strains, was similar for both groups, except in the case of Proteus, that was found in 10 cases with breast milk, and in only 3 cases with cow's milk. Breast milk was unable to avoid the colonization by E. coli and in one case, was unable to eradicate this germ. None of the cases fed breast milk had diarrhea, including the three cases with E. coli. In another group, 4 cases developed severe acute diarrhea, due to enteropathogenic E. coli followed by 2 deaths. The results seem to point out that although colonization by enteropathogenic strains of E. coli is not completely avoided, these strains do not cause disease when these infants are fed breast milk.", "contents": "[Maternal lactation. II. Intestinal colonization in breast fed newborn infants]. Twenty-three low birth weight infants were studied to establish the role that breast milk plays in the intestinal colonization of the preterm infant, and in the control of epidemic diarrhea due to enteropathogenic E. coli, in nurseries. Twelve of these case were fed breast milk in bottles, and eleven with \"humanized\" powder cow's milk (NAN M.R.). Bacteriologic studies of feces were performed daily during the first 18 days of life. In 5 cases given breast milk, and in 8 receiving cow's milk, Staphylococcus aureus was isolated. Enteropathogenic E. coli was found in 3 cases with breast milk and in 6 with cow's milk. The finding of other non-pathogenic strains, was similar for both groups, except in the case of Proteus, that was found in 10 cases with breast milk, and in only 3 cases with cow's milk. Breast milk was unable to avoid the colonization by E. coli and in one case, was unable to eradicate this germ. None of the cases fed breast milk had diarrhea, including the three cases with E. coli. In another group, 4 cases developed severe acute diarrhea, due to enteropathogenic E. coli followed by 2 deaths. The results seem to point out that although colonization by enteropathogenic strains of E. coli is not completely avoided, these strains do not cause disease when these infants are fed breast milk."}
{"id": "PMID:465176", "title": "[Bronchial asthma in children under 2 years of age].", "content": "Bronchial asthma is defined and some basic physiopathological phenomena are quoted. The most important facts leading to the investigation and the possibility of establishing the diagnosis of bronchial asthma in infants are mentioned. 61 children under 2 years of age who had been taken for consultation to the Bronchial Pulmonary Disease Department of the Exequiel Gonz\u00e1lez Cort\u00e9s Hospital and the Child Medical Institute were analyzed in connection with the contribution of the anamnesis, physical check-up and the laboratory test to the diagnosis of the bronchial asthma. The following items are emphasized: value of the clinical history, the clinical test of total reversability of bronchial obstruction to (towards) adrenaline and the esoinophil count in bronchial secretion, and the skin test in children over 12 months of age. The conclusion is that it is possible to diagnose bronchial asthma in the child under two years old through simple, specific methods in any medical center with minimal equipment.", "contents": "[Bronchial asthma in children under 2 years of age]. Bronchial asthma is defined and some basic physiopathological phenomena are quoted. The most important facts leading to the investigation and the possibility of establishing the diagnosis of bronchial asthma in infants are mentioned. 61 children under 2 years of age who had been taken for consultation to the Bronchial Pulmonary Disease Department of the Exequiel Gonz\u00e1lez Cort\u00e9s Hospital and the Child Medical Institute were analyzed in connection with the contribution of the anamnesis, physical check-up and the laboratory test to the diagnosis of the bronchial asthma. The following items are emphasized: value of the clinical history, the clinical test of total reversability of bronchial obstruction to (towards) adrenaline and the esoinophil count in bronchial secretion, and the skin test in children over 12 months of age. The conclusion is that it is possible to diagnose bronchial asthma in the child under two years old through simple, specific methods in any medical center with minimal equipment."}
{"id": "PMID:465177", "title": "[Vascular rings].", "content": "22 cases of several types of vascular rings are presented. Most of them were double aortic arch (15): 12 were operated on without mortality before the first years of age because of the great severity of the symptoms. The precocity of the symptomatology and the predominance of the respiratory distress is emphasized. Our data point out to the importance of the differential diagnosis of vascular rings in newborns and infants in the presence of laryngeal stridor or respiratory distress, essentially because the surgical correction is very effective and the risk of the intervention is minimal.", "contents": "[Vascular rings]. 22 cases of several types of vascular rings are presented. Most of them were double aortic arch (15): 12 were operated on without mortality before the first years of age because of the great severity of the symptoms. The precocity of the symptomatology and the predominance of the respiratory distress is emphasized. Our data point out to the importance of the differential diagnosis of vascular rings in newborns and infants in the presence of laryngeal stridor or respiratory distress, essentially because the surgical correction is very effective and the risk of the intervention is minimal."}
{"id": "PMID:465180", "title": "[Rheumatic fever prevention unit].", "content": "The author describes the steps that have been taken recently on the prevention and the magnitude of rheumatic fever in Chile. Likewise, he describes the measures proposed by the Advising Committee on Rheumatic Fever, the evolution this disease has followed and the goals that they expect to reach before the next Pan American Conference on Rheumatic Fever.", "contents": "[Rheumatic fever prevention unit]. The author describes the steps that have been taken recently on the prevention and the magnitude of rheumatic fever in Chile. Likewise, he describes the measures proposed by the Advising Committee on Rheumatic Fever, the evolution this disease has followed and the goals that they expect to reach before the next Pan American Conference on Rheumatic Fever."}
{"id": "PMID:465179", "title": "[Cholestyramine in the treatment of acute bacterial diarrhea in infants].", "content": "32 infants admitted to Hospital \"Roberto del R\u00edo\" with infectious diarrhea and dehydration were assigned random to two groups, one of them receiving the usual therapeutic regimen of rehydration, refeeding and furazolidone and the other group the same treatment plus cholestyramine. The patients were studied with balance technique on days 1st, 3rd., and 5th. after admission, analyzing clinical progress, features of the stools, total digestive transit time, pH of the feces and the presence of reducing substances, volume of the stools, water excretion and sodium, potassium and chloride elimination in the feces. The patients receiving cholestyramine showed an earlier improvement in the appearance of the stools, less excretion of feces and water and diminished sodium and potassium elimination. Chloride excretion was similar in both groups. Cholestyramine tolerance was excellent. No side effects or complications were observed.", "contents": "[Cholestyramine in the treatment of acute bacterial diarrhea in infants]. 32 infants admitted to Hospital \"Roberto del R\u00edo\" with infectious diarrhea and dehydration were assigned random to two groups, one of them receiving the usual therapeutic regimen of rehydration, refeeding and furazolidone and the other group the same treatment plus cholestyramine. The patients were studied with balance technique on days 1st, 3rd., and 5th. after admission, analyzing clinical progress, features of the stools, total digestive transit time, pH of the feces and the presence of reducing substances, volume of the stools, water excretion and sodium, potassium and chloride elimination in the feces. The patients receiving cholestyramine showed an earlier improvement in the appearance of the stools, less excretion of feces and water and diminished sodium and potassium elimination. Chloride excretion was similar in both groups. Cholestyramine tolerance was excellent. No side effects or complications were observed."}
{"id": "PMID:465178", "title": "[Environment, nutrition and intellectual development].", "content": "The differential effects of malnutrition and psychosocial cultural deprivation (PSAC) upon intellectual performance and psychomotor development were studied in 52 preschool children attending nursery schools in urban Santiago (Chile). Nutritional status was evaluated according to G\u00f3mez, intellectual performance according to Weschler's scale (WISP and WISC tests), psychomotor development according to the Denver Developmental Screening Test and PSAC according to a numerical scale constructed from maternal I.Q.; socioeconomic status (Graffar) and a psychological questionnaire the correlational analysis, undernutrition was assigned a single value calculated from the severity and duration of the disease. An inverse correlation of - 0.36 existed between malnutrition and intellectual performance, and of - 0.39 between malnutrition and psychomotor development. Also, there was an inverse and significant correlation of - 0.73 between PSAC and psychomotor development; there was no correlation between PSAC and intellectual performance. Verbal I.Q. was more affected (r = - 0.52) than manual I.Q. (r = - 0.18) and children malnourished during the first 6 months of life attained lower scores of I.Q. than normals. The duration of malnutrition (mild in all cases) had no influence upon subsequent I.Q. This study was the basis for a rehabilitation program (educational-nutritional) to be applied in preschool children from nonprivileged areas.", "contents": "[Environment, nutrition and intellectual development]. The differential effects of malnutrition and psychosocial cultural deprivation (PSAC) upon intellectual performance and psychomotor development were studied in 52 preschool children attending nursery schools in urban Santiago (Chile). Nutritional status was evaluated according to G\u00f3mez, intellectual performance according to Weschler's scale (WISP and WISC tests), psychomotor development according to the Denver Developmental Screening Test and PSAC according to a numerical scale constructed from maternal I.Q.; socioeconomic status (Graffar) and a psychological questionnaire the correlational analysis, undernutrition was assigned a single value calculated from the severity and duration of the disease. An inverse correlation of - 0.36 existed between malnutrition and intellectual performance, and of - 0.39 between malnutrition and psychomotor development. Also, there was an inverse and significant correlation of - 0.73 between PSAC and psychomotor development; there was no correlation between PSAC and intellectual performance. Verbal I.Q. was more affected (r = - 0.52) than manual I.Q. (r = - 0.18) and children malnourished during the first 6 months of life attained lower scores of I.Q. than normals. The duration of malnutrition (mild in all cases) had no influence upon subsequent I.Q. This study was the basis for a rehabilitation program (educational-nutritional) to be applied in preschool children from nonprivileged areas."}
{"id": "PMID:465182", "title": "[Urinary infection and pyelonephritis].", "content": "Actual concepts on urinary tract infection (UTI) and pyelonephritis have changed constantly through the years. A brief historical review has been considered in this paper, with the hope that nowadays concepts may help the clinician towards a better diagnostic and management approach of these still so common clinical disorders. Bacteriological studies were performed in 245 urine samples obtained by three different procedures, in order to evaluate the reability of each technique, which were: different procedures, in order to evaluate the reability of each technique, which were: a) suprapubic puncture; b) single culture obtained by midstream voiding technique, and c) serial cultures in three different samples, also obtained by the latter technique. E. coli was isolated in 91% of the initial diagnosed episode of UTI, and in 87% of the recurrences. Same bacteria, as well as other organisms and mixed flora were isolated from patients with predisposing factors present within the urinary tract (intra UT predisposing factor). Results show that suprapubic tap is the most reliable technique, since bacterial contamination of the sample is avoided. Fifty percent incidence of false positive results was found when a single culture by midstream voiding technique was used. A better correlation between suprapubic puncture and serial cultures obtained by midstream voiding technique was found. Both procedures are recommended. Predisposing factors, mainly within the urinary tract, were found in 68% of the cases with demonstrated UTI. Vesicoureteral reflux was found in 27% from the total number of patients studied, and in 52% of the patients with intra-UT predisposing factors. Suggestions are made in relationship with diagnostic approach and management of patients with UTI, in order to reveal the presence of predisposing factors, since they are the main feature to take into account regarding UTI, location of the site of infection, recurrences, chronic evolution, treatment and prognosis.", "contents": "[Urinary infection and pyelonephritis]. Actual concepts on urinary tract infection (UTI) and pyelonephritis have changed constantly through the years. A brief historical review has been considered in this paper, with the hope that nowadays concepts may help the clinician towards a better diagnostic and management approach of these still so common clinical disorders. Bacteriological studies were performed in 245 urine samples obtained by three different procedures, in order to evaluate the reability of each technique, which were: different procedures, in order to evaluate the reability of each technique, which were: a) suprapubic puncture; b) single culture obtained by midstream voiding technique, and c) serial cultures in three different samples, also obtained by the latter technique. E. coli was isolated in 91% of the initial diagnosed episode of UTI, and in 87% of the recurrences. Same bacteria, as well as other organisms and mixed flora were isolated from patients with predisposing factors present within the urinary tract (intra UT predisposing factor). Results show that suprapubic tap is the most reliable technique, since bacterial contamination of the sample is avoided. Fifty percent incidence of false positive results was found when a single culture by midstream voiding technique was used. A better correlation between suprapubic puncture and serial cultures obtained by midstream voiding technique was found. Both procedures are recommended. Predisposing factors, mainly within the urinary tract, were found in 68% of the cases with demonstrated UTI. Vesicoureteral reflux was found in 27% from the total number of patients studied, and in 52% of the patients with intra-UT predisposing factors. Suggestions are made in relationship with diagnostic approach and management of patients with UTI, in order to reveal the presence of predisposing factors, since they are the main feature to take into account regarding UTI, location of the site of infection, recurrences, chronic evolution, treatment and prognosis."}
{"id": "PMID:465183", "title": "[Study of the immune system in relatives of children with dermatomyositis].", "content": "In view of the fact that relatives of patient with other connective tissue diseases may show immunologic abnormalities, we investigated cellular (PPD, SK-SD, candidine and diphteria-tetanous toxoid cutaneous tests) and humoral (IgG, IgM, IgA. CH50, C3, rheumatoid factor and ANA) immune responses in first degrees relatives (parents and siblings) of children with dermatomyositis. Of the 53 subjects tested, 19 had high values of one or two immunoglobulines; however, the mean Ig value was not different from that of the general population (p greater than 0.1), CH50, C3, rheumatoid factor and ANA were normal or negative. Cutaneous tests made on 46 subjects did not reveal abnormalities. Therefore our conclusion is that first degree relatives of children with dermatomyositis have not significant immunologic abnormalities.", "contents": "[Study of the immune system in relatives of children with dermatomyositis]. In view of the fact that relatives of patient with other connective tissue diseases may show immunologic abnormalities, we investigated cellular (PPD, SK-SD, candidine and diphteria-tetanous toxoid cutaneous tests) and humoral (IgG, IgM, IgA. CH50, C3, rheumatoid factor and ANA) immune responses in first degrees relatives (parents and siblings) of children with dermatomyositis. Of the 53 subjects tested, 19 had high values of one or two immunoglobulines; however, the mean Ig value was not different from that of the general population (p greater than 0.1), CH50, C3, rheumatoid factor and ANA were normal or negative. Cutaneous tests made on 46 subjects did not reveal abnormalities. Therefore our conclusion is that first degree relatives of children with dermatomyositis have not significant immunologic abnormalities."}
{"id": "PMID:465184", "title": "[Intraventricular therapy in the management of ventricular ependymitis].", "content": "Through Scott's tubes, intraventricular administration of amynoglucoside antibiotics was given to two groups of patients: a) ten children with purulent meningitis and ventricular ependymitis due to gram - negative germs without malformation of the central nervous system (CNS) and b) sixteen children with ventricular ependymitis and malformation of the CNS. The duration of treatment was three weeks and no deaths issued in the first group. In the second group, there were four therapeutic failures related with the existence of encephalodysplasia or intraventricular wailing. The results described are very encouraging and allow to foresee a marked drop in the lethality of ventricular ependymitis.", "contents": "[Intraventricular therapy in the management of ventricular ependymitis]. Through Scott's tubes, intraventricular administration of amynoglucoside antibiotics was given to two groups of patients: a) ten children with purulent meningitis and ventricular ependymitis due to gram - negative germs without malformation of the central nervous system (CNS) and b) sixteen children with ventricular ependymitis and malformation of the CNS. The duration of treatment was three weeks and no deaths issued in the first group. In the second group, there were four therapeutic failures related with the existence of encephalodysplasia or intraventricular wailing. The results described are very encouraging and allow to foresee a marked drop in the lethality of ventricular ependymitis."}
{"id": "PMID:465181", "title": "[Acute pulmonary edema at high altitude in Mexico City].", "content": "For the first time in Mexico, two cases of acute pulmonary edema of high altitude are reported in a 6 year -old child and in a 16 year- old adolescent; both children lived in the city of Mexico. In identical circumstances, both developed progressive acute respiratory insufficiency on their return to Mexico City after several days at sea level. The final diagnosis was acute pulmonary edema of high altitude. In this paper, the producing mechanisms of this complication are discussed, together with the importance of making it known, in order to put on the lookout the medical personel, who work in the plateau, so that they may be conscious that this disturbance may occur at altitudes like that of Mexico City, (2480 meters above sea level) which is much lower than the altitude considered dangerous. (3000 meters). The most important facts of the clinical picture, together with the treatment recommended are pointed out.", "contents": "[Acute pulmonary edema at high altitude in Mexico City]. For the first time in Mexico, two cases of acute pulmonary edema of high altitude are reported in a 6 year -old child and in a 16 year- old adolescent; both children lived in the city of Mexico. In identical circumstances, both developed progressive acute respiratory insufficiency on their return to Mexico City after several days at sea level. The final diagnosis was acute pulmonary edema of high altitude. In this paper, the producing mechanisms of this complication are discussed, together with the importance of making it known, in order to put on the lookout the medical personel, who work in the plateau, so that they may be conscious that this disturbance may occur at altitudes like that of Mexico City, (2480 meters above sea level) which is much lower than the altitude considered dangerous. (3000 meters). The most important facts of the clinical picture, together with the treatment recommended are pointed out."}
{"id": "PMID:465185", "title": "[Placental pathology and perinatal infection].", "content": "The purpose of this review is to call attention on the participation of the placenta in intrauterine infections. The importance of a prompt recognition of the latter and the value of the placental examination to the understanding of such problems affecting the fetus and the newborn are emphasized.", "contents": "[Placental pathology and perinatal infection]. The purpose of this review is to call attention on the participation of the placenta in intrauterine infections. The importance of a prompt recognition of the latter and the value of the placental examination to the understanding of such problems affecting the fetus and the newborn are emphasized."}
{"id": "PMID:465186", "title": "[Short stature and nephrogenic diabetes insipidus. Report of a clinical case].", "content": "A male child who was initially studied as a short stature syndrome, was found to have a severe picture of polyuria-polydipsia. The routine tests for dehydration and vasopressin administration were carried out and the results obtained were fully demonstrative of a lack of renal response to the administration of vasopressin.", "contents": "[Short stature and nephrogenic diabetes insipidus. Report of a clinical case]. A male child who was initially studied as a short stature syndrome, was found to have a severe picture of polyuria-polydipsia. The routine tests for dehydration and vasopressin administration were carried out and the results obtained were fully demonstrative of a lack of renal response to the administration of vasopressin."}
{"id": "PMID:465188", "title": "[Epidermolysis bullosa of the newborn].", "content": "Nine cases of epidermolysis bullosa of the newborn are reported. Three cases corresponded to the simple type; 4 were dystrophic and two belonged to the letal phase. Those of the letal variety died from septicemia, while simple and dystrophic varieties are under hospital control at the out-patient department.", "contents": "[Epidermolysis bullosa of the newborn]. Nine cases of epidermolysis bullosa of the newborn are reported. Three cases corresponded to the simple type; 4 were dystrophic and two belonged to the letal phase. Those of the letal variety died from septicemia, while simple and dystrophic varieties are under hospital control at the out-patient department."}
{"id": "PMID:465187", "title": "[Engelmann-Camurati's disease. Report of a clinical case and its evolution during 4 years].", "content": "Engelmann-Camurati's diseases is a progressive alteration of the diaphysis of the long bones, where the etiology is unknown. It appears clinically around the age of two years, showing weakness of the lower limbs and frequent falls; there is also weight loss and in older patients, the appearance of secondary sexual characteristics is retarded. The diagnosis is made clinically and corroborated with x-ray studies that show increased density and width of the diaphysis. We report a case in a female where the diagnosis was established at twelve years of age; she received steroid therapy for four years without improvement. The mechanism of action of the steroids is unknown, but it seems that they stimulate the formation of healthy bone with adequate remodelling. Physiotherapy is necessary in these patients and if there should be a compression of the cranial nerves, surgical management may be useful to relieve compression.", "contents": "[Engelmann-Camurati's disease. Report of a clinical case and its evolution during 4 years]. Engelmann-Camurati's diseases is a progressive alteration of the diaphysis of the long bones, where the etiology is unknown. It appears clinically around the age of two years, showing weakness of the lower limbs and frequent falls; there is also weight loss and in older patients, the appearance of secondary sexual characteristics is retarded. The diagnosis is made clinically and corroborated with x-ray studies that show increased density and width of the diaphysis. We report a case in a female where the diagnosis was established at twelve years of age; she received steroid therapy for four years without improvement. The mechanism of action of the steroids is unknown, but it seems that they stimulate the formation of healthy bone with adequate remodelling. Physiotherapy is necessary in these patients and if there should be a compression of the cranial nerves, surgical management may be useful to relieve compression."}
{"id": "PMID:465190", "title": "[Acute toxoplasmic myelitis in the newborns].", "content": "The present paper describes 3 patients with congenital toxoplasmosis. The spinal cord was extremely affected. Differential diagnoses were established with poliomyelitis and neonatal hypoxia. Histopathological studies revealed toxoplasmosis.", "contents": "[Acute toxoplasmic myelitis in the newborns]. The present paper describes 3 patients with congenital toxoplasmosis. The spinal cord was extremely affected. Differential diagnoses were established with poliomyelitis and neonatal hypoxia. Histopathological studies revealed toxoplasmosis."}
{"id": "PMID:465189", "title": "[Perinatal morbimortality].", "content": "Between 1972 and 1975 (4 years), the Hospital de Ginecoobstetricia \"Dr. Ignacio Morones Prieto\" of the I.M.S.S. at Monterrey, N.L., reported a birth rate of 106, 771 with 98.35% (105,004) live borns. The morbidity rate was 262.06/1000 live borns and the perinatal mortality was 31.24/1000, divided into antenatal 16.54/1000 and posnatal 14.70/1000. Within morbidity, prematurity and/or low weight occupies the first place, followed by respiratory pathology and afterwards, jaundice, infections, obstetric trauma and finally, congenital malformations. The neonatal mortality reached 14.70/1000 live borns and if we consider gestational age, 22.9% corresponds to immature products, 50.2% prematures and 26.0% to full-term products. In relation to hours of life, we find that 62.50% of the total number of deaths were reported in products less than 24 hrs. of life and 37.50%, in products over 24 hrs. of life.", "contents": "[Perinatal morbimortality]. Between 1972 and 1975 (4 years), the Hospital de Ginecoobstetricia \"Dr. Ignacio Morones Prieto\" of the I.M.S.S. at Monterrey, N.L., reported a birth rate of 106, 771 with 98.35% (105,004) live borns. The morbidity rate was 262.06/1000 live borns and the perinatal mortality was 31.24/1000, divided into antenatal 16.54/1000 and posnatal 14.70/1000. Within morbidity, prematurity and/or low weight occupies the first place, followed by respiratory pathology and afterwards, jaundice, infections, obstetric trauma and finally, congenital malformations. The neonatal mortality reached 14.70/1000 live borns and if we consider gestational age, 22.9% corresponds to immature products, 50.2% prematures and 26.0% to full-term products. In relation to hours of life, we find that 62.50% of the total number of deaths were reported in products less than 24 hrs. of life and 37.50%, in products over 24 hrs. of life."}
{"id": "PMID:465196", "title": "[Histopathological study on experimental cerebral infarction in rat (author's transl)].", "content": "An experimental model of cerebral infarction in rat brain was successfully established by means of the unilateral internal carotid injection of carbon microspheres. A histopathological investigation of the infarcted brain was performed. The results were as follow: 1. No cerebral infarction was made by the unilateral internal carotid ligation alone. 2. Focal cerebral edema was most marked at the white matter of the embolized hemisphere. 3. The edema was also conspicuous in the deep cerebral gray matter, especially in hippocampus. Degeneration and pyknosis of the neurons were observed at the same area. 4. Transudative hemorrhage in the focal cerebral edema was usually observed in the thalamus. 5. Edematous change in the cortical gray matter was not so marked as compared with the hippocampus and thalamus. 6. Carbon microsphere was not found in the capillaries of ipsilateral choroid plexus. The present experimental model of cerebral infarction will be useful in elucidating the pathological mechanisms of the cerebral infarction.", "contents": "[Histopathological study on experimental cerebral infarction in rat (author's transl)]. An experimental model of cerebral infarction in rat brain was successfully established by means of the unilateral internal carotid injection of carbon microspheres. A histopathological investigation of the infarcted brain was performed. The results were as follow: 1. No cerebral infarction was made by the unilateral internal carotid ligation alone. 2. Focal cerebral edema was most marked at the white matter of the embolized hemisphere. 3. The edema was also conspicuous in the deep cerebral gray matter, especially in hippocampus. Degeneration and pyknosis of the neurons were observed at the same area. 4. Transudative hemorrhage in the focal cerebral edema was usually observed in the thalamus. 5. Edematous change in the cortical gray matter was not so marked as compared with the hippocampus and thalamus. 6. Carbon microsphere was not found in the capillaries of ipsilateral choroid plexus. The present experimental model of cerebral infarction will be useful in elucidating the pathological mechanisms of the cerebral infarction."}
{"id": "PMID:465191", "title": "[Surgical closure of the patent ductus arteriosus in preterm newborns].", "content": "Two cases of patent ductus arteriosus in preterm newborns who were operated on are reported. Several physiopathologic and clinical aspects of importance for the diagnosis and treatment of this entity are pointed out.", "contents": "[Surgical closure of the patent ductus arteriosus in preterm newborns]. Two cases of patent ductus arteriosus in preterm newborns who were operated on are reported. Several physiopathologic and clinical aspects of importance for the diagnosis and treatment of this entity are pointed out."}
{"id": "PMID:465192", "title": "[Renal failure and rickets].", "content": "Acute renal failure secondary to interstitial nephritis caused by therapeutic ingestion of sodium diphenylhydantoins has been reported recently. The interference of sodium diphenylhydantoins on Vitamin D metabolism causing or aggravating ricketts has also been reported. This communication deals with an infant girl who was admitted to the hospital due to seizures. Four months before, she had convulsions and she was treated with diphenylhydantoins until admission. She was found to have renal failure and ricketts. Histological diagnosis of interstitial nephritis was established by means of percutaneous renal biopsy. Clinical and radiological improvement of ricketts was observed after dehydrotachysterol treatment. Clinical and biochemical alterations of renal failure slowly subsided. She had a clear-cut history of vitamin D defficiency ricketts. Seizures were due to hypocalcemia tetany but was erroneusly treated as \"grand mal\" epilepsy, with diphenylhydantoins. Interstitial nephritis complicated with acute renal failure was probably caused by diphenylhydantoins administration.", "contents": "[Renal failure and rickets]. Acute renal failure secondary to interstitial nephritis caused by therapeutic ingestion of sodium diphenylhydantoins has been reported recently. The interference of sodium diphenylhydantoins on Vitamin D metabolism causing or aggravating ricketts has also been reported. This communication deals with an infant girl who was admitted to the hospital due to seizures. Four months before, she had convulsions and she was treated with diphenylhydantoins until admission. She was found to have renal failure and ricketts. Histological diagnosis of interstitial nephritis was established by means of percutaneous renal biopsy. Clinical and radiological improvement of ricketts was observed after dehydrotachysterol treatment. Clinical and biochemical alterations of renal failure slowly subsided. She had a clear-cut history of vitamin D defficiency ricketts. Seizures were due to hypocalcemia tetany but was erroneusly treated as \"grand mal\" epilepsy, with diphenylhydantoins. Interstitial nephritis complicated with acute renal failure was probably caused by diphenylhydantoins administration."}
{"id": "PMID:465193", "title": "[Capillaria hepatica. Report of the 2nd cased found in the Mexican Republic].", "content": "The 2nd. case of Capillaria hepatica seen in the Mexican Republic is reported together with clinical and laboratory findings of the visceral larva migrans syndrome, diagnosis which was made at the Hospital of Specialties of the Mexican Social Security Institute at Puebla, Pue. Mex. Same considerations are made regarding the biological cycle, its morphological characteristics and a review of the present state of the pathology, evolution and prognosis of the illness is made, focusing the attention to an immunological trend, as a close origin of the illness. Thus, it is concluded that an early recognition and diagnosis leads to an a adequate treatment that will be benifical to this kind of patients.", "contents": "[Capillaria hepatica. Report of the 2nd cased found in the Mexican Republic]. The 2nd. case of Capillaria hepatica seen in the Mexican Republic is reported together with clinical and laboratory findings of the visceral larva migrans syndrome, diagnosis which was made at the Hospital of Specialties of the Mexican Social Security Institute at Puebla, Pue. Mex. Same considerations are made regarding the biological cycle, its morphological characteristics and a review of the present state of the pathology, evolution and prognosis of the illness is made, focusing the attention to an immunological trend, as a close origin of the illness. Thus, it is concluded that an early recognition and diagnosis leads to an a adequate treatment that will be benifical to this kind of patients."}
{"id": "PMID:465194", "title": "[The battered child syndrome. Preliminary review in a Bolivian pediatric hospital].", "content": "Taking into account the prevalence of child abuse in countries with different socioeconomic levels, the authors review this entity through the casuistics in a Bolivian pediatric hospital. Besides confirming unquestionable clinical findings, it was observed that among twenty-seven cases there was a predominance in preschool and school age children, in contrast with previous papers. The finding of three children with burns of various degrees and two cases who died as consequence of child abuse must be emplasized. The authors comment the varying forms of this syndrome according with foregoing Occidental publications, accepting that non-physical forms should require prospective researches in order to define a creditable prevalence and incidence of child abuse in an exposed population.", "contents": "[The battered child syndrome. Preliminary review in a Bolivian pediatric hospital]. Taking into account the prevalence of child abuse in countries with different socioeconomic levels, the authors review this entity through the casuistics in a Bolivian pediatric hospital. Besides confirming unquestionable clinical findings, it was observed that among twenty-seven cases there was a predominance in preschool and school age children, in contrast with previous papers. The finding of three children with burns of various degrees and two cases who died as consequence of child abuse must be emplasized. The authors comment the varying forms of this syndrome according with foregoing Occidental publications, accepting that non-physical forms should require prospective researches in order to define a creditable prevalence and incidence of child abuse in an exposed population."}
{"id": "PMID:465195", "title": "[Cellulitis in pediatrics. Diagnostic and therapeutic considerations].", "content": "The clinical histories of 180 cases of cellulitis or phlegmona diffusa were studied at the Hospital de Pediatr\u00eda del Centro Medico Nacional. The disease prevailed in infants and preschool children. Staphylococcus aureus was the etiological agent most frequently found, but with the presence of enterobacteriaceae in 39% of patients under 2 years of age. One third of the children with cellulitis showed one or several complications. Lethality reached 5% (9 cases), but always related to septicemia from S. aureus. In 14.4%, osteoarthritis was present; frequently there was: fever for over 5 days in spite of adequate treatment, a history of late initiation of the antimicrobial drug (over 1 week) and phlogosis or functional limitation. The radiological picture that shows the bony lesion was evident only after two weeks. One half these cases remained with sequelae. Considerations are made on the adequate plans for antibiotic treatment and the early diagnosis of the osteoarticular complication.", "contents": "[Cellulitis in pediatrics. Diagnostic and therapeutic considerations]. The clinical histories of 180 cases of cellulitis or phlegmona diffusa were studied at the Hospital de Pediatr\u00eda del Centro Medico Nacional. The disease prevailed in infants and preschool children. Staphylococcus aureus was the etiological agent most frequently found, but with the presence of enterobacteriaceae in 39% of patients under 2 years of age. One third of the children with cellulitis showed one or several complications. Lethality reached 5% (9 cases), but always related to septicemia from S. aureus. In 14.4%, osteoarthritis was present; frequently there was: fever for over 5 days in spite of adequate treatment, a history of late initiation of the antimicrobial drug (over 1 week) and phlogosis or functional limitation. The radiological picture that shows the bony lesion was evident only after two weeks. One half these cases remained with sequelae. Considerations are made on the adequate plans for antibiotic treatment and the early diagnosis of the osteoarticular complication."}
{"id": "PMID:465202", "title": "[Cerebral infarction due to vasospasm following intracranial aneurysm rupture (author's transl)].", "content": "We have made an investigation of the 827 cases of ruptured saccular cerebral aneurysms operated on and 151 of them presented infarction attacks thought to be due to vasospasm brought about by aneurysm rupture. Based upon symptoms, angiography and surgical findings, an analysis was made of their age, sex and aneurysm location so related to incidence of infarction, period from the initial or last rupturing until time of infarction, indication of vasospasms from angiographical findings, and symptoms of infarction. Infarction occurs only between the fourth and fifteenth days following the last bleeding and in this period there was a high incidence of rebleeding, so that the current conception of reattack following cerebral aneurysm rupture must be updated in light of the fact that the therapy for rerupture and the therapy for infarction due to vasospasm are completely different.", "contents": "[Cerebral infarction due to vasospasm following intracranial aneurysm rupture (author's transl)]. We have made an investigation of the 827 cases of ruptured saccular cerebral aneurysms operated on and 151 of them presented infarction attacks thought to be due to vasospasm brought about by aneurysm rupture. Based upon symptoms, angiography and surgical findings, an analysis was made of their age, sex and aneurysm location so related to incidence of infarction, period from the initial or last rupturing until time of infarction, indication of vasospasms from angiographical findings, and symptoms of infarction. Infarction occurs only between the fourth and fifteenth days following the last bleeding and in this period there was a high incidence of rebleeding, so that the current conception of reattack following cerebral aneurysm rupture must be updated in light of the fact that the therapy for rerupture and the therapy for infarction due to vasospasm are completely different."}
{"id": "PMID:465203", "title": "[On the phenomena of multiple extravasations of contrast medium in cerebral angiogram of the cases of hypertensive intracerebral hematoma (author's transl)].", "content": "It is interesting to note the pathological findings that the rupture of multiple microaneurysms in the basal ganglia appears to be the cause of the hypertensive intracerebral hematoma. But there is few report that the carotid angiogram demonstrated multiple leakages of contrast medium of intracerebral hematoma in vivo than in Westberg's postmortem angiogram. The authors experienced 15 cases of hypertensive intracerebral hematoma whose cerebral angiogram showed extravasation of contrast material. It was fifty-seven per cent of which angiogram within 6 hours after the stroke showed leakage of contrast medium. However, there was remarkably decreased appearance of the extravasation after 9 hours after the stroke. Moreover, emphasis showed be placed upon the facts that eleven cases of 15 cases showed more than two adjacent spotty extravasations of contrast medium and that six cases demonstrated the simultaneous multiple leakages of contrast material from more than two lentriculostriate arteries. These angiographic findings has led us to the conclusion that there is intimate correlation between the large hematoma and multiple ruptures of the microaneurysms of lentriculostriate arteries.", "contents": "[On the phenomena of multiple extravasations of contrast medium in cerebral angiogram of the cases of hypertensive intracerebral hematoma (author's transl)]. It is interesting to note the pathological findings that the rupture of multiple microaneurysms in the basal ganglia appears to be the cause of the hypertensive intracerebral hematoma. But there is few report that the carotid angiogram demonstrated multiple leakages of contrast medium of intracerebral hematoma in vivo than in Westberg's postmortem angiogram. The authors experienced 15 cases of hypertensive intracerebral hematoma whose cerebral angiogram showed extravasation of contrast material. It was fifty-seven per cent of which angiogram within 6 hours after the stroke showed leakage of contrast medium. However, there was remarkably decreased appearance of the extravasation after 9 hours after the stroke. Moreover, emphasis showed be placed upon the facts that eleven cases of 15 cases showed more than two adjacent spotty extravasations of contrast medium and that six cases demonstrated the simultaneous multiple leakages of contrast material from more than two lentriculostriate arteries. These angiographic findings has led us to the conclusion that there is intimate correlation between the large hematoma and multiple ruptures of the microaneurysms of lentriculostriate arteries."}
{"id": "PMID:465205", "title": "Abnormal left ventricular wall movement in patients with chest pain and normal coronary arteriograms. Relation to inferior T wave changes and mitral prolapse.", "content": "Left ventriculograms of 45 patients with angina and normal coronary arteriograms were digitised frame by frame in order to detect regional abnormalities of wall movement. Though left ventricular pressures, end-diastolic volume, and ejection fraction were normal in all, regional outward movement during early systole was present in 10 patients, and abnormal inward wall movement during isovolumic relaxation also in 10, involving the apex or inferior surface. Both were present together in 8 patients, and affected segments showed normal amplitude and peak velocity of movement during ejection. These disturbances of wall movement were associated with inferior T wave changes on the electrocardiogram, and mitral prolapse, particularly when the latter resulted from delayed movement of the valve during ejection. It is suggested that the onset of contraction is delayed in affected areas, but that it proceeds normally thereafter. The resulting persistence of tension into the period of relaxation of the remainder of the ventricle may interfere locally with coronary flow, particularly during tachycardia, thus causing manifestations of regional ischaemia.", "contents": "Abnormal left ventricular wall movement in patients with chest pain and normal coronary arteriograms. Relation to inferior T wave changes and mitral prolapse. Left ventriculograms of 45 patients with angina and normal coronary arteriograms were digitised frame by frame in order to detect regional abnormalities of wall movement. Though left ventricular pressures, end-diastolic volume, and ejection fraction were normal in all, regional outward movement during early systole was present in 10 patients, and abnormal inward wall movement during isovolumic relaxation also in 10, involving the apex or inferior surface. Both were present together in 8 patients, and affected segments showed normal amplitude and peak velocity of movement during ejection. These disturbances of wall movement were associated with inferior T wave changes on the electrocardiogram, and mitral prolapse, particularly when the latter resulted from delayed movement of the valve during ejection. It is suggested that the onset of contraction is delayed in affected areas, but that it proceeds normally thereafter. The resulting persistence of tension into the period of relaxation of the remainder of the ventricle may interfere locally with coronary flow, particularly during tachycardia, thus causing manifestations of regional ischaemia."}
{"id": "PMID:465207", "title": "Echocardiographic pattern of posterior mitral valve leaflet movement after mitral valve repair.", "content": "The pattern of movement of the posterior leaflet at the time of opening of the stenosed mitral valve has been described as either anterior or posterior in direction. In 32 patients who underwent mitral annuloplasty, the anatomical features of the mitral apparatus were correlated with the postoperative echocardiographic pattern of movements of the posterior leaflet. Anterior movement of the posterior leaflet was associated with extensive disease of this leaflet, and posterior movement with minimal disease. No relation was found between valve orifice or anterior leaflet mobility and the pattern of movement of the posterior leaflet.", "contents": "Echocardiographic pattern of posterior mitral valve leaflet movement after mitral valve repair. The pattern of movement of the posterior leaflet at the time of opening of the stenosed mitral valve has been described as either anterior or posterior in direction. In 32 patients who underwent mitral annuloplasty, the anatomical features of the mitral apparatus were correlated with the postoperative echocardiographic pattern of movements of the posterior leaflet. Anterior movement of the posterior leaflet was associated with extensive disease of this leaflet, and posterior movement with minimal disease. No relation was found between valve orifice or anterior leaflet mobility and the pattern of movement of the posterior leaflet."}
{"id": "PMID:465208", "title": "Mitral stenosis with high left ventricular diastolic pressure.", "content": "Three patients with mitral stenosis are described, in whom the haemodynamic findings at cardiac catheterisation were more suggestive of left ventricular myocardial disease, in that the left ventricular diastolic pressure was high and the mitral valve gradient small. However, their echocardiograms showed abnormal wall movement during diastole characteristic of severe inflow obstruction, with slow and protracted filling, and at operation mitral stenosis was confirmed. Left ventricular wall stress was estimated throughout the cardiac cycle in one patient, and the diastolic stress-strain relation shown to be abnormal. The effects of mitral stenosis on left ventricular function are complex, and are not explicable simply by reduction in size of the mitral orifice.", "contents": "Mitral stenosis with high left ventricular diastolic pressure. Three patients with mitral stenosis are described, in whom the haemodynamic findings at cardiac catheterisation were more suggestive of left ventricular myocardial disease, in that the left ventricular diastolic pressure was high and the mitral valve gradient small. However, their echocardiograms showed abnormal wall movement during diastole characteristic of severe inflow obstruction, with slow and protracted filling, and at operation mitral stenosis was confirmed. Left ventricular wall stress was estimated throughout the cardiac cycle in one patient, and the diastolic stress-strain relation shown to be abnormal. The effects of mitral stenosis on left ventricular function are complex, and are not explicable simply by reduction in size of the mitral orifice."}
{"id": "PMID:465210", "title": "Measuring the effect of a mobile coronary care unit upon the community.", "content": "A mobile coronary care unit working within a geographically defined area retrieved 20% of the estimated number of cases with myocardial infarction. These cases were similar to the population at risk in terms of age, sex, and previous history of ischaemic heart disease. Patients retrieved by the mobile unit had a lower overall case fatality ratio. This discrepancy was greater than could be explained by resuscitation or preventive treatment by the mobile unit teams. Reasons for this difference in mortality were explored. The benefits of the mobile coronary care unit were obvious in terms of the existing service but small in terms of the community. Any judgment on the effectiveness of a mobile unit must use both approaches to get a balanced view.", "contents": "Measuring the effect of a mobile coronary care unit upon the community. A mobile coronary care unit working within a geographically defined area retrieved 20% of the estimated number of cases with myocardial infarction. These cases were similar to the population at risk in terms of age, sex, and previous history of ischaemic heart disease. Patients retrieved by the mobile unit had a lower overall case fatality ratio. This discrepancy was greater than could be explained by resuscitation or preventive treatment by the mobile unit teams. Reasons for this difference in mortality were explored. The benefits of the mobile coronary care unit were obvious in terms of the existing service but small in terms of the community. Any judgment on the effectiveness of a mobile unit must use both approaches to get a balanced view."}
{"id": "PMID:465212", "title": "Patterns of haemodynamic alteration during left ventricular ischaemia in man. Relation to angiographic extent of coronary artery disease.", "content": "Haemodynamic changes produced by rapid atrial pacing (60 patients, 52 of whom developed angina) or in association with spontaneous angina (32 patients) were measured in 92 patients with angiographic coronary artery disease. The extent of coronary artery disease was scored by the jeopardy score system (range 0 to 12). The haemodynamic changes induced by ischaemia occurred in 3 patterns: pattern I, no change in filling pressure or in mean systemic arterial pressure; pattern II, a rise in filling pressure and a rise in mean systemic arterial pressure; pattern III, a rise in filling pressure, but no significant change or a fall in mean systemic arterial pressure. In patients who had a pattern II or a pattern III response to ischaemia, the change in mean systemic arterial pressure was linearly related to the corresponding change in cardiac output. The likelihood of a patient showing a given pattern of ischaemia-induced haemodynamic change was related to the extent of coronary artery disease; of 22 patients with jeopardy scores of 2 or 4, 91% exhibited pattern I, 9% pattern II, and none pattern III; of 39 patients with jeopardy scores of 6 or 8, 40% exhibited pattern I, 22% pattern II, and 38% pattern III; of 31 patients with jeopardy scores of 10 or 12, 12% exhibited pattern I, 10% pattern II, and 78% pattern III (P less than 0.01). Among the 54 patients in whom serial cardiac output determinations were available, a decline of the left ventricular function curve during ischaemia was demonstrable in 8% of those with a pattern I response, in 54% of those with a pattern II response, and in 90% of those with a pattern III response (P less than 0.01). The pattern of response was unrelated to resting angiographic left ventricular ejection fraction, whether ST segments became elevated or depressed, or whether ischaemia was pacing-induced or spontaneous. These results suggest that the haemodynamic response to ischaemia is determined by the fraction of the left ventricle that becomes dysfunctional during ischaemia.", "contents": "Patterns of haemodynamic alteration during left ventricular ischaemia in man. Relation to angiographic extent of coronary artery disease. Haemodynamic changes produced by rapid atrial pacing (60 patients, 52 of whom developed angina) or in association with spontaneous angina (32 patients) were measured in 92 patients with angiographic coronary artery disease. The extent of coronary artery disease was scored by the jeopardy score system (range 0 to 12). The haemodynamic changes induced by ischaemia occurred in 3 patterns: pattern I, no change in filling pressure or in mean systemic arterial pressure; pattern II, a rise in filling pressure and a rise in mean systemic arterial pressure; pattern III, a rise in filling pressure, but no significant change or a fall in mean systemic arterial pressure. In patients who had a pattern II or a pattern III response to ischaemia, the change in mean systemic arterial pressure was linearly related to the corresponding change in cardiac output. The likelihood of a patient showing a given pattern of ischaemia-induced haemodynamic change was related to the extent of coronary artery disease; of 22 patients with jeopardy scores of 2 or 4, 91% exhibited pattern I, 9% pattern II, and none pattern III; of 39 patients with jeopardy scores of 6 or 8, 40% exhibited pattern I, 22% pattern II, and 38% pattern III; of 31 patients with jeopardy scores of 10 or 12, 12% exhibited pattern I, 10% pattern II, and 78% pattern III (P less than 0.01). Among the 54 patients in whom serial cardiac output determinations were available, a decline of the left ventricular function curve during ischaemia was demonstrable in 8% of those with a pattern I response, in 54% of those with a pattern II response, and in 90% of those with a pattern III response (P less than 0.01). The pattern of response was unrelated to resting angiographic left ventricular ejection fraction, whether ST segments became elevated or depressed, or whether ischaemia was pacing-induced or spontaneous. These results suggest that the haemodynamic response to ischaemia is determined by the fraction of the left ventricle that becomes dysfunctional during ischaemia."}
{"id": "PMID:465214", "title": "Pulmonary hypertension and sudden death in aortic stenosis.", "content": "Sudden death is now an infrequent occurrence in severe aortic stenosis. However, an impressive increase in pulmonary arteriolar resistance has been found in some patients with end-stage aortic stenosis dying suddenly or deteriorating suddenly after catheterisation. Pulmonary hypertension does not seem to cause sudden death, but, in conjunction with decreased cardiac output, a critical reduction in aortic orifice area, and left ventricular failure, pulmonary hypertension identifies a population at significant risk. The rare finding of severe pulmonary hypertension in aortic stenosis should be considered an important marker for sudden death and in association with left ventricular failure may indicate an urgent need for valve replacement, regardless of the apparent clinical condition of the patient. In a small number of subjects catheterised postoperatively, increased pulmonary arteriolar resistance lessened rapidly.", "contents": "Pulmonary hypertension and sudden death in aortic stenosis. Sudden death is now an infrequent occurrence in severe aortic stenosis. However, an impressive increase in pulmonary arteriolar resistance has been found in some patients with end-stage aortic stenosis dying suddenly or deteriorating suddenly after catheterisation. Pulmonary hypertension does not seem to cause sudden death, but, in conjunction with decreased cardiac output, a critical reduction in aortic orifice area, and left ventricular failure, pulmonary hypertension identifies a population at significant risk. The rare finding of severe pulmonary hypertension in aortic stenosis should be considered an important marker for sudden death and in association with left ventricular failure may indicate an urgent need for valve replacement, regardless of the apparent clinical condition of the patient. In a small number of subjects catheterised postoperatively, increased pulmonary arteriolar resistance lessened rapidly."}
{"id": "PMID:465216", "title": "Doerr's theory of morphogenesis of arterial transposition in light of recent research.", "content": "Doerr's theory of the morphogenesis of transposition is discussed with special reference to recent studies by Goor and co-workers and Anderson and associates. The views advanced by all these authors coincide in three points: (a) the description of the reorganisation process occurring at the arterial end of the embryonic heart (a process called by Doerr vectorial bulbus rotation); (b) the pathogenetic interpretation of transposition as the result of an arrest of vectorial bulbus rotation; (c) the recognition of a teratological series or spectrum of anomalies pathogenetically related to transposition. Vectorial bulbus rotation is explained mainly as the result of three largely simultaneous events; bulbar shift, bulbus torsion, and truncus torsion. The spectrum of anomalies related to transposition appears as a close-knit series. Bulbar retraction does not seem to be a necessary condition for the connection of the aorta to the left ventricle.", "contents": "Doerr's theory of morphogenesis of arterial transposition in light of recent research. Doerr's theory of the morphogenesis of transposition is discussed with special reference to recent studies by Goor and co-workers and Anderson and associates. The views advanced by all these authors coincide in three points: (a) the description of the reorganisation process occurring at the arterial end of the embryonic heart (a process called by Doerr vectorial bulbus rotation); (b) the pathogenetic interpretation of transposition as the result of an arrest of vectorial bulbus rotation; (c) the recognition of a teratological series or spectrum of anomalies pathogenetically related to transposition. Vectorial bulbus rotation is explained mainly as the result of three largely simultaneous events; bulbar shift, bulbus torsion, and truncus torsion. The spectrum of anomalies related to transposition appears as a close-knit series. Bulbar retraction does not seem to be a necessary condition for the connection of the aorta to the left ventricle."}
{"id": "PMID:465217", "title": "Gross distortion of atrioventricular and ventriculo-arterial relations associated with left juxtaposition of atrial appendages. Bizarre form of atrioventricular criss-cross.", "content": "Two pathological specimens are described in which a bizarre distortion of atrioventricular and ventriculoarterial relations existed. In both cases anterior and leftward displacement of the tricuspid valve coexisted with rightward displacement of the infundibular regions and semilunar valves. Abnormal expansion of the AV canal, possibly secondary to malrotation of the cardiac tube, is considered as a possible cause of the anomaly. One of the hearts showed severe subpulmonary stenosis produced by a valve-like structure which was well differentiated, with a complete annulus and chordae tendineae that inserted in a parachute-like fashion on the infundibular septum. The morphology of this structure was clearly different from that fo the fibrous tags described in other anomalies. Its origin is uncertain. These anomalies have important implications in relation to the nomenclature and classification of congenital heart malformations, and illustrate the value of recent modifications of the segmental approach.", "contents": "Gross distortion of atrioventricular and ventriculo-arterial relations associated with left juxtaposition of atrial appendages. Bizarre form of atrioventricular criss-cross. Two pathological specimens are described in which a bizarre distortion of atrioventricular and ventriculoarterial relations existed. In both cases anterior and leftward displacement of the tricuspid valve coexisted with rightward displacement of the infundibular regions and semilunar valves. Abnormal expansion of the AV canal, possibly secondary to malrotation of the cardiac tube, is considered as a possible cause of the anomaly. One of the hearts showed severe subpulmonary stenosis produced by a valve-like structure which was well differentiated, with a complete annulus and chordae tendineae that inserted in a parachute-like fashion on the infundibular septum. The morphology of this structure was clearly different from that fo the fibrous tags described in other anomalies. Its origin is uncertain. These anomalies have important implications in relation to the nomenclature and classification of congenital heart malformations, and illustrate the value of recent modifications of the segmental approach."}
{"id": "PMID:465218", "title": "Elective aortic and mitral valve surgery in patients over 70 years of age.", "content": "The results of elective cardiac surgery carried out between 1970 and 1975 on 29 patients over 70 years of age are reported. Operative mortality was 10%, late cardiac mortality 14%, with 72% survival at a mean follow-up of 38.6 months. Before operation 96% were in functional classes 3 and 4. After operation 90.5% of late survivors were in functional classes 1 and 2 at a mean follow-up of 43.5 months. These results compare favourably with those in younger patients. Complication rate and length of hospital stay were unremarkable. The risks of neurological damage and of long-term anticoagulation are not increased. Emergency surgery carries a very high risk in this age group. The importance of biological youth and good left ventricular function is emphasised. For aortic stenosis at least prognosis is improved by surgery.", "contents": "Elective aortic and mitral valve surgery in patients over 70 years of age. The results of elective cardiac surgery carried out between 1970 and 1975 on 29 patients over 70 years of age are reported. Operative mortality was 10%, late cardiac mortality 14%, with 72% survival at a mean follow-up of 38.6 months. Before operation 96% were in functional classes 3 and 4. After operation 90.5% of late survivors were in functional classes 1 and 2 at a mean follow-up of 43.5 months. These results compare favourably with those in younger patients. Complication rate and length of hospital stay were unremarkable. The risks of neurological damage and of long-term anticoagulation are not increased. Emergency surgery carries a very high risk in this age group. The importance of biological youth and good left ventricular function is emphasised. For aortic stenosis at least prognosis is improved by surgery."}
{"id": "PMID:465219", "title": "Sinus node dysfunction associated with absence of right superior vena cava.", "content": "A 41-year-old man presented with dizziness associated with sinus bradycardia and sinus arrest. An attempt to implant a transvenous pacing lead was frustrated by absence of the right superior vena cava. The left superior vena cava persisted and drained via the coronary sinus into the right atrium. Absence of the right superior vena cava may present with symptomatic sinus node dysfunction and may require an epicardial demand pacing system.", "contents": "Sinus node dysfunction associated with absence of right superior vena cava. A 41-year-old man presented with dizziness associated with sinus bradycardia and sinus arrest. An attempt to implant a transvenous pacing lead was frustrated by absence of the right superior vena cava. The left superior vena cava persisted and drained via the coronary sinus into the right atrium. Absence of the right superior vena cava may present with symptomatic sinus node dysfunction and may require an epicardial demand pacing system."}
{"id": "PMID:465220", "title": "A case of isolated primary chylopericardium.", "content": "Primary chylopericardium presented as radiographic cardiomegaly in an asymptomatic 19-year-old man. Normal findings at cardiac catheterisation and angiographic demonstration of cardiac displacement from the diaphragm suggested a collection of fluid below the heart which was confirmed by M and B mode ultrasound scanning. Thoracic blood pool isotope scanning indicated that the lumen was a pericardial effusion rather than a cyst. Lymphangiography did not indicate any direct lymphatic communication though a small pool of contrast appeared in the pericardium and the diagnosis of chylopericardium was confirmed by pericardiocentesis. Surgical treatment was undertaken after rapid reaccumulation of chyle and the patient remains well 6 months later.", "contents": "A case of isolated primary chylopericardium. Primary chylopericardium presented as radiographic cardiomegaly in an asymptomatic 19-year-old man. Normal findings at cardiac catheterisation and angiographic demonstration of cardiac displacement from the diaphragm suggested a collection of fluid below the heart which was confirmed by M and B mode ultrasound scanning. Thoracic blood pool isotope scanning indicated that the lumen was a pericardial effusion rather than a cyst. Lymphangiography did not indicate any direct lymphatic communication though a small pool of contrast appeared in the pericardium and the diagnosis of chylopericardium was confirmed by pericardiocentesis. Surgical treatment was undertaken after rapid reaccumulation of chyle and the patient remains well 6 months later."}
{"id": "PMID:465221", "title": "Rotational excursion of heart in massive pericardial effusion studied by phased-array echocardiography.", "content": "We have observed rotational excursion, comprising anteroposterior and right to left movement of the heart, and cardiac twist around the long axis in 2 patients with massive pericardial effusion, using a phased-array echocardiograph recently developed in this country. Sagittal real-time cross-sections showed only the anteroposterior swing motion, but horizontal cross-sections revealed a counterclockwise rotational excursion and cardiac twist during this rotational excursion. The rotational excursion consisted of a rapid and almost straight movement right and posteriorly in systole, and a slow arcing excursion left and anteriorly in diastole. The speed of this movement was not uniform. The heart disclosed dominant counterclockwise twisting motions in mid to late systole and in late diastole, synchronous with atrial contraction. These movements were described relative to an external system of co-ordinates. Controls, consisting of a normal healthy subject, and cases with mitral stenosis, mitral regurgitation, and hypertrophic cardiomyopathy, did not show any rotational excursion. In the latter 2 diseases, cardiac twists were observed, but they were less dominant than in cases with massive pericardial effusion.", "contents": "Rotational excursion of heart in massive pericardial effusion studied by phased-array echocardiography. We have observed rotational excursion, comprising anteroposterior and right to left movement of the heart, and cardiac twist around the long axis in 2 patients with massive pericardial effusion, using a phased-array echocardiograph recently developed in this country. Sagittal real-time cross-sections showed only the anteroposterior swing motion, but horizontal cross-sections revealed a counterclockwise rotational excursion and cardiac twist during this rotational excursion. The rotational excursion consisted of a rapid and almost straight movement right and posteriorly in systole, and a slow arcing excursion left and anteriorly in diastole. The speed of this movement was not uniform. The heart disclosed dominant counterclockwise twisting motions in mid to late systole and in late diastole, synchronous with atrial contraction. These movements were described relative to an external system of co-ordinates. Controls, consisting of a normal healthy subject, and cases with mitral stenosis, mitral regurgitation, and hypertrophic cardiomyopathy, did not show any rotational excursion. In the latter 2 diseases, cardiac twists were observed, but they were less dominant than in cases with massive pericardial effusion."}
{"id": "PMID:465222", "title": "Evaluation of posterior aortic wall echogram in diagnosis of mitral valve disease.", "content": "The clinical usefulness of detecting abnormal movement of the posterior wall of the aortic root by M-mode echocardiography was studied in 93 patients with common cardiac diseases (mitral and aortic valve disease, atrial septal defect, hypertrophic and congestive cardiomyopathy) and in 17 normal subjects. Though abnormally slow (less than 3 cm/s) or prolonged (greater than 240 ms) diastolic movement was confirmed to be common in mitral stenosis, since it occurred in 35 of 36 patients it was non-specific. Similar abnormalities frequently occurred in other patients with, for example, mitral regurgitation, aortic valve disease, after aortic valve replacement, and in both hypertrophic and congestive cardiomyopathy. The severity of mitral stenosis, assessed at operation, could not be accurately predicted from abnormalities of aortic root movement. Information derived from aortic movement was not diagnostic and did not predict severity in isolated mitral regurgitation though both the peak rate of systolic aortic motion and total aortic excursion were significantly greater than normal. We conclude that abnormalities of posterior aortic wall movement are frequent and their specificity and clinical usefulness are limited.", "contents": "Evaluation of posterior aortic wall echogram in diagnosis of mitral valve disease. The clinical usefulness of detecting abnormal movement of the posterior wall of the aortic root by M-mode echocardiography was studied in 93 patients with common cardiac diseases (mitral and aortic valve disease, atrial septal defect, hypertrophic and congestive cardiomyopathy) and in 17 normal subjects. Though abnormally slow (less than 3 cm/s) or prolonged (greater than 240 ms) diastolic movement was confirmed to be common in mitral stenosis, since it occurred in 35 of 36 patients it was non-specific. Similar abnormalities frequently occurred in other patients with, for example, mitral regurgitation, aortic valve disease, after aortic valve replacement, and in both hypertrophic and congestive cardiomyopathy. The severity of mitral stenosis, assessed at operation, could not be accurately predicted from abnormalities of aortic root movement. Information derived from aortic movement was not diagnostic and did not predict severity in isolated mitral regurgitation though both the peak rate of systolic aortic motion and total aortic excursion were significantly greater than normal. We conclude that abnormalities of posterior aortic wall movement are frequent and their specificity and clinical usefulness are limited."}
{"id": "PMID:465223", "title": "Determination of pressure gradient in mitral stenosis with Doppler echocardiography.", "content": "The accuracy of a non-invasive ultrasound Doppler technique for the determination of the pressure gradient in mitral stenosis was evaluated in a study of 8 adult patients. Transseptal left atrial catheterisation and retrograde left ventricular catheterisation were performed. The same diastoles were used to compare the gradient constructed from the ultrasound data (delta PU) with that constructed from the manometric data (delta PM). In the 8 patients the difference betweent he mean diastolic values of delta PU and delta PM was - 0.54 +/- 1.0 (SD) mmHg. The corresponding figure for mid-diastole was 0.01 +/- 0.9 (SD) mmHg. The results indicate that the ultrasound technique is sufficiently accurate for diagnostic purposes.", "contents": "Determination of pressure gradient in mitral stenosis with Doppler echocardiography. The accuracy of a non-invasive ultrasound Doppler technique for the determination of the pressure gradient in mitral stenosis was evaluated in a study of 8 adult patients. Transseptal left atrial catheterisation and retrograde left ventricular catheterisation were performed. The same diastoles were used to compare the gradient constructed from the ultrasound data (delta PU) with that constructed from the manometric data (delta PM). In the 8 patients the difference betweent he mean diastolic values of delta PU and delta PM was - 0.54 +/- 1.0 (SD) mmHg. The corresponding figure for mid-diastole was 0.01 +/- 0.9 (SD) mmHg. The results indicate that the ultrasound technique is sufficiently accurate for diagnostic purposes."}
{"id": "PMID:465224", "title": "Nomenclature and classification of congenital heart disease.", "content": "At present there is no universally accepted nomenclature for congenital cardiac malformations. Much of the controversy results from failure to distinguish the structural connections of the heart from the morphology and spatial relations of its components. The confusion is compounded by an abundance of individual definitions, many of them speculative. The present article proposes a totally descriptive nomenclature. It describes in turn the connections of the cardiac segments, their morphology, their relations, and additional anomalies in any segment. Each step in the segmental approach is discrete. The overall effect is to force a succinct and comprehensive description of any cardiac malformation, no matter how complex.", "contents": "Nomenclature and classification of congenital heart disease. At present there is no universally accepted nomenclature for congenital cardiac malformations. Much of the controversy results from failure to distinguish the structural connections of the heart from the morphology and spatial relations of its components. The confusion is compounded by an abundance of individual definitions, many of them speculative. The present article proposes a totally descriptive nomenclature. It describes in turn the connections of the cardiac segments, their morphology, their relations, and additional anomalies in any segment. Each step in the segmental approach is discrete. The overall effect is to force a succinct and comprehensive description of any cardiac malformation, no matter how complex."}
{"id": "PMID:465225", "title": "Prevalence of congenital heart disease in black schoolchildren of Soweto, Johannesburg.", "content": "A survey conducted by cardiologists in Soweto, Johannesburg, provided an opportunity of assessing the frequency of congenital heart disease in black schoolchildren. Among 12,050 schoolchildren aged 2 to 18 years, 48 had a congenital heart defect, yielding a prevalence of 3.9 per 1000. Only in 2- to 6-year-old children did the prevalence exceed that of rheumatic heart disease. The distribution of the types of defects was largely similar to that reported in other surveys with a predominance (52%) of ventricular septal defects. Two unusual findings were the unexplained absence of persistent ductus arteriosus in these children, and the detection of 5 children with situs inversus (1 in 2410). In all but one child, the congenital heart defect was first discovered during the survey. Despite the limitations of a prevalence study, it can be concluded that congenital heart disease is at least as common in this South African black community as in Caucasians.", "contents": "Prevalence of congenital heart disease in black schoolchildren of Soweto, Johannesburg. A survey conducted by cardiologists in Soweto, Johannesburg, provided an opportunity of assessing the frequency of congenital heart disease in black schoolchildren. Among 12,050 schoolchildren aged 2 to 18 years, 48 had a congenital heart defect, yielding a prevalence of 3.9 per 1000. Only in 2- to 6-year-old children did the prevalence exceed that of rheumatic heart disease. The distribution of the types of defects was largely similar to that reported in other surveys with a predominance (52%) of ventricular septal defects. Two unusual findings were the unexplained absence of persistent ductus arteriosus in these children, and the detection of 5 children with situs inversus (1 in 2410). In all but one child, the congenital heart defect was first discovered during the survey. Despite the limitations of a prevalence study, it can be concluded that congenital heart disease is at least as common in this South African black community as in Caucasians."}
{"id": "PMID:465230", "title": "Haemodynamic studies in veno-occlusive disease of the liver.", "content": "Haemodynamic studies in 8 tribal patients from central India with veno-occlusive disease of the liver are reported. The disease was caused by eating cereals contaminated with seeds of a crotalaria species rich in toxic pyrrolizidine alkaloids similar to monocrotaline and fulvine. All patients showed raised hepatic venous wedge pressures and pressure gradients between hepatic venous wedge and free hepatic vein. Significant pressure gradients were also observed in the inferior vena cava. Right and left heart pressures were normal. Cardiac and stroke indices and ventricular work were significantly increased while systemic and pulmonary resistances were reduced. Hepatic blood flow was also reduced. Hepatic venograms were abnormal and the changes correlated well with the pressure gradients between hepatic venous wedge and free hepatic vein and with the histological changes in the liver.", "contents": "Haemodynamic studies in veno-occlusive disease of the liver. Haemodynamic studies in 8 tribal patients from central India with veno-occlusive disease of the liver are reported. The disease was caused by eating cereals contaminated with seeds of a crotalaria species rich in toxic pyrrolizidine alkaloids similar to monocrotaline and fulvine. All patients showed raised hepatic venous wedge pressures and pressure gradients between hepatic venous wedge and free hepatic vein. Significant pressure gradients were also observed in the inferior vena cava. Right and left heart pressures were normal. Cardiac and stroke indices and ventricular work were significantly increased while systemic and pulmonary resistances were reduced. Hepatic blood flow was also reduced. Hepatic venograms were abnormal and the changes correlated well with the pressure gradients between hepatic venous wedge and free hepatic vein and with the histological changes in the liver."}
{"id": "PMID:465231", "title": "Electrophysiological and haemodynamic basis for application of new pacemaker technology in sick sinus syndrome and atrioventricular block.", "content": "A fundamental description of pacemaker systems which are commercially available or in clinical validation is given as a background for their application in a series of 62 consecutive patients presenting over a period of 1 year for permanent cardiac pacing. The patients (23 (37%) sick sinus syndrome, 38 (61%) atrioventricular block, and 1 ventricular tachycardia) were studied electrophysiologically and haemodynamically to allow the appropriate application of a pacemaker system. In sick sinus syndrome, 8 patients had permanent atrial pacing, 14 ventricular pacing, and 1 atrioventricular sequential pacing; in atrioventricular block, 8 patients had atrial synchronous ventricular inhibited pacing and the remaining 30 had ventricular pacing. A high incidence of atrial fibrillation, 9 patients, and abnormal sinus node function, 15 patients, precluded wider use of atrial synchrony. The results show benefit in acute haemodynamic studies of using systems including atrial sensing and/or pacing, and with greater availability of atrioventricular sequential and still more advanced pacemakers with dual sensing as well as dual pacing the majority of patients may be offered this benefit.", "contents": "Electrophysiological and haemodynamic basis for application of new pacemaker technology in sick sinus syndrome and atrioventricular block. A fundamental description of pacemaker systems which are commercially available or in clinical validation is given as a background for their application in a series of 62 consecutive patients presenting over a period of 1 year for permanent cardiac pacing. The patients (23 (37%) sick sinus syndrome, 38 (61%) atrioventricular block, and 1 ventricular tachycardia) were studied electrophysiologically and haemodynamically to allow the appropriate application of a pacemaker system. In sick sinus syndrome, 8 patients had permanent atrial pacing, 14 ventricular pacing, and 1 atrioventricular sequential pacing; in atrioventricular block, 8 patients had atrial synchronous ventricular inhibited pacing and the remaining 30 had ventricular pacing. A high incidence of atrial fibrillation, 9 patients, and abnormal sinus node function, 15 patients, precluded wider use of atrial synchrony. The results show benefit in acute haemodynamic studies of using systems including atrial sensing and/or pacing, and with greater availability of atrioventricular sequential and still more advanced pacemakers with dual sensing as well as dual pacing the majority of patients may be offered this benefit."}
{"id": "PMID:465232", "title": "Cardiomyopathic syndrome caused by coronary artery disease. III: Prospective clinicopathological study of its prevalence among patients with clinically unexplained chronic heart failure.", "content": "Each day, for one year, the medical records of adult patients who died in hospital were reviewed before seeing the necropsy findings. For those patients who had had chronic left or left and right heart failure, a presumptive cause was assigned on the basis of antemortem clinical data. Of 740 consecutive patients who were studied at necropsy, 90 had had chronic heart failure. In 15 patients the cause of heart failure was not apparent by clinical criteria; of these, 7 were found at necropsy to have cardiomyopathic syndrome caused by coronary artery disease. In retrospect, the presence of overt diabetes mellitus was a clue that cardiomyopathy caused by coronary artery disease was the cause of clinically unexplained heart failure; 5 of 7 patients with unexplained heart failure who were found to have this at necropsy were diabetic, whereas only 1 of the other 8 patients with clinically unexplained heart failure was diabetic (P less than 0.05). Patients in whom clinically unexplained heart failure was found to be the result of cardiomyopathy caused by coronary artery disease had multiple myocardial infarctions on pathological examination, which, with one exception, were nontransmural. By contrast, myocardial infarctions were transmural on pathological examination in each of 7 matched 'controls' with heart failure, in whom the diagnosis of coronary artery disease had been clinically apparent (P less than 0.01).", "contents": "Cardiomyopathic syndrome caused by coronary artery disease. III: Prospective clinicopathological study of its prevalence among patients with clinically unexplained chronic heart failure. Each day, for one year, the medical records of adult patients who died in hospital were reviewed before seeing the necropsy findings. For those patients who had had chronic left or left and right heart failure, a presumptive cause was assigned on the basis of antemortem clinical data. Of 740 consecutive patients who were studied at necropsy, 90 had had chronic heart failure. In 15 patients the cause of heart failure was not apparent by clinical criteria; of these, 7 were found at necropsy to have cardiomyopathic syndrome caused by coronary artery disease. In retrospect, the presence of overt diabetes mellitus was a clue that cardiomyopathy caused by coronary artery disease was the cause of clinically unexplained heart failure; 5 of 7 patients with unexplained heart failure who were found to have this at necropsy were diabetic, whereas only 1 of the other 8 patients with clinically unexplained heart failure was diabetic (P less than 0.05). Patients in whom clinically unexplained heart failure was found to be the result of cardiomyopathy caused by coronary artery disease had multiple myocardial infarctions on pathological examination, which, with one exception, were nontransmural. By contrast, myocardial infarctions were transmural on pathological examination in each of 7 matched 'controls' with heart failure, in whom the diagnosis of coronary artery disease had been clinically apparent (P less than 0.01)."}
{"id": "PMID:465233", "title": "Marfanoid hypermobility syndrome associated with coarctation of the aorta.", "content": "There are intimate interrelations between various elements of connective tissue, viz. collagen, elastin, and glycoproteins. It is not unexpected that Marfan and Ehlers-Danlos syndromes share common features. The condition is labelled as Marfanoid hypermobility syndrome. In the patient described here, the Marfanoid hypermobility syndrome was associated with coarctation of the aorta which was corrected surgically.", "contents": "Marfanoid hypermobility syndrome associated with coarctation of the aorta. There are intimate interrelations between various elements of connective tissue, viz. collagen, elastin, and glycoproteins. It is not unexpected that Marfan and Ehlers-Danlos syndromes share common features. The condition is labelled as Marfanoid hypermobility syndrome. In the patient described here, the Marfanoid hypermobility syndrome was associated with coarctation of the aorta which was corrected surgically."}
{"id": "PMID:465234", "title": "Polymyositis and complete heart block.", "content": "The development of complete heart block is described in a patient with polymyositis. The implantation of a permanent pacemaker has controlled the heart block, but the progression of the underlying disease despite treatment with relatively high dosage of corticosteroids makes the outlook uncertain.", "contents": "Polymyositis and complete heart block. The development of complete heart block is described in a patient with polymyositis. The implantation of a permanent pacemaker has controlled the heart block, but the progression of the underlying disease despite treatment with relatively high dosage of corticosteroids makes the outlook uncertain."}
{"id": "PMID:465237", "title": "Prognosis of patients paced for chronic atrioventricular block.", "content": "Between the years 1960 and 1974, 839 patients were paced for chronic complete atrioventricular block. Analysis of survival compared with the general population showed that 170 deaths were expected according to standard mortality tables and 288 actually occurred, giving a ratio of actual to expected deaths of 1.7:1. Patients with a definite history of myocardial infarction showed a higher than average mortality when paced. Mortality was not influenced whether heart was constant or intermittent, whether the ventricular rate was below or above 40/minutes, or whether QRS duration was greater or less than 0.1 second. Analysis of the age groups paced disclosed the most important correlations. Between the ages of 80 and 89 years paced patients could expect to survive as long as other of the same age without heart block. There was, however, a very high ratio of 4.5:1 for 90 patients in the age group 50 to 59 years. The reason for the high mortality ratio was uncertain but it may have been the result of a greater incidence of underlying coronary artery disease.", "contents": "Prognosis of patients paced for chronic atrioventricular block. Between the years 1960 and 1974, 839 patients were paced for chronic complete atrioventricular block. Analysis of survival compared with the general population showed that 170 deaths were expected according to standard mortality tables and 288 actually occurred, giving a ratio of actual to expected deaths of 1.7:1. Patients with a definite history of myocardial infarction showed a higher than average mortality when paced. Mortality was not influenced whether heart was constant or intermittent, whether the ventricular rate was below or above 40/minutes, or whether QRS duration was greater or less than 0.1 second. Analysis of the age groups paced disclosed the most important correlations. Between the ages of 80 and 89 years paced patients could expect to survive as long as other of the same age without heart block. There was, however, a very high ratio of 4.5:1 for 90 patients in the age group 50 to 59 years. The reason for the high mortality ratio was uncertain but it may have been the result of a greater incidence of underlying coronary artery disease."}
{"id": "PMID:465238", "title": "Complete right bundle-branch block: echophonocardiographic study of first heart sound and right ventricular contraction times.", "content": "High speed enchocardiograms of the mitral, tricuspid, and pulmonary valves were recorded with a simultaneous electrocardiogram and phonocardiogram in 20 patients with complete right bundle-branch block and in 67 normal subjects. Late opening of the pulmonary valve indicating late right ventricular ejection was found in all patients. In 8 patients with wide splitting of the first heart sound the late ejection was related mainly to delay in tricuspid valve closure, suggesting a late onset of the right ventricular pressure pulse. In 10 patients with a single first heart sound the delayed ejection was associated with a long interval between tricuspid valve closure and pulmonary valve opening, suggesting a slow rising right ventricular pressure pulse; 3 of these patients also had late tricuspid valve closure but the tricuspid component of the first sound was absent. Late onset of pressure rise is thought to result from block in the main right bundle-branch, and a slow rising pulse from block in the distal Purkinje network. These findings explain the conflicting results in previous studies of the first heart sound and right ventricular pressure pulse in patients with right bundle-branch block, and may have prognostic significance.", "contents": "Complete right bundle-branch block: echophonocardiographic study of first heart sound and right ventricular contraction times. High speed enchocardiograms of the mitral, tricuspid, and pulmonary valves were recorded with a simultaneous electrocardiogram and phonocardiogram in 20 patients with complete right bundle-branch block and in 67 normal subjects. Late opening of the pulmonary valve indicating late right ventricular ejection was found in all patients. In 8 patients with wide splitting of the first heart sound the late ejection was related mainly to delay in tricuspid valve closure, suggesting a late onset of the right ventricular pressure pulse. In 10 patients with a single first heart sound the delayed ejection was associated with a long interval between tricuspid valve closure and pulmonary valve opening, suggesting a slow rising right ventricular pressure pulse; 3 of these patients also had late tricuspid valve closure but the tricuspid component of the first sound was absent. Late onset of pressure rise is thought to result from block in the main right bundle-branch, and a slow rising pulse from block in the distal Purkinje network. These findings explain the conflicting results in previous studies of the first heart sound and right ventricular pressure pulse in patients with right bundle-branch block, and may have prognostic significance."}
{"id": "PMID:465239", "title": "Patients treated in a coronary care unit without acute myocardial infarction: identification of high risk subgroup for subsequent myocardial infarction and/or cardiovascular death.", "content": "Consecutive patients admitted to a coronary care unit (CCU) during one year were studied. The diagnosis of acute myocardial infarction was not substantiated by our criteria in 206 of the patients discharged from the CCU. Of these, 193 were retrospectively followed up during one year. Seventeen of the patients (9%) died from cardiovascular causes during the 1-year period. Another 14 patients (7%) had a subsequent non-fatal acute myocardial infarction during the same period. The majority of the patients had coronary artery disease. Only 32 (17%) could be classified as non-coronary cases, and these had an excellent prognosis without any subsequent acute myocardial infarctions or deaths. The occurrence of transient ST-T shifts in serial electrocardiograms obtained during the first 3 days in hospital selected a subgroup of patients who had a high risk for subsequent non-fatal acute myocardial infarction and/or cardiovascular death. This high risk subgroup provides a basis for more aggressive diagnostic and therapeutic intervention.", "contents": "Patients treated in a coronary care unit without acute myocardial infarction: identification of high risk subgroup for subsequent myocardial infarction and/or cardiovascular death. Consecutive patients admitted to a coronary care unit (CCU) during one year were studied. The diagnosis of acute myocardial infarction was not substantiated by our criteria in 206 of the patients discharged from the CCU. Of these, 193 were retrospectively followed up during one year. Seventeen of the patients (9%) died from cardiovascular causes during the 1-year period. Another 14 patients (7%) had a subsequent non-fatal acute myocardial infarction during the same period. The majority of the patients had coronary artery disease. Only 32 (17%) could be classified as non-coronary cases, and these had an excellent prognosis without any subsequent acute myocardial infarctions or deaths. The occurrence of transient ST-T shifts in serial electrocardiograms obtained during the first 3 days in hospital selected a subgroup of patients who had a high risk for subsequent non-fatal acute myocardial infarction and/or cardiovascular death. This high risk subgroup provides a basis for more aggressive diagnostic and therapeutic intervention."}
{"id": "PMID:465240", "title": "Reduction of early ventricular arrhythmia by acebutolol in patients with acute myocardial infarction.", "content": "To assess the effects of intravenously administered acebutolol (1-20 mg every 4 hours for 24 hours) on cardiac rhythm and performance, we studied 72 patients with evolving myocardial infarction. Twenty-five patients were treated with acebutolol beginning 6 hours after the first increase in the level of plasma creatine kinase. Enzymatically estimated infarct size was compared with that of 25 controls matched for predicted infarct size. Observed infarct sizes were not significantly different in the 2 groups (37 +/- 5 and 30 +/- 5 CK-gram equivalents, respectively). Mean heart rate, diastolic blood pressure, and cardiac output declined from control values during treatment with acebutolol, but remained within the normal range. Mean pulmonary artery pressure and pulmonary artery occlusive pressure were unchanged. In a group of 22 treated patients matched with 22 control subjects for frequency of ventricular extrasystoles, acebutolol effected a prompt reduction in frequencies of ventricular extrasystoles and repetitive arrhythmias, whereas values were not significantly changed in controls during the corresponding intervals. Accordingly, acebutolol may be a useful antiarrhythmic agent in selected patients with acute myocardial infarction with adversely altering haemodynamic stability or enzymatically estimated infarct size.", "contents": "Reduction of early ventricular arrhythmia by acebutolol in patients with acute myocardial infarction. To assess the effects of intravenously administered acebutolol (1-20 mg every 4 hours for 24 hours) on cardiac rhythm and performance, we studied 72 patients with evolving myocardial infarction. Twenty-five patients were treated with acebutolol beginning 6 hours after the first increase in the level of plasma creatine kinase. Enzymatically estimated infarct size was compared with that of 25 controls matched for predicted infarct size. Observed infarct sizes were not significantly different in the 2 groups (37 +/- 5 and 30 +/- 5 CK-gram equivalents, respectively). Mean heart rate, diastolic blood pressure, and cardiac output declined from control values during treatment with acebutolol, but remained within the normal range. Mean pulmonary artery pressure and pulmonary artery occlusive pressure were unchanged. In a group of 22 treated patients matched with 22 control subjects for frequency of ventricular extrasystoles, acebutolol effected a prompt reduction in frequencies of ventricular extrasystoles and repetitive arrhythmias, whereas values were not significantly changed in controls during the corresponding intervals. Accordingly, acebutolol may be a useful antiarrhythmic agent in selected patients with acute myocardial infarction with adversely altering haemodynamic stability or enzymatically estimated infarct size."}
{"id": "PMID:465241", "title": "Estimation of myocardial infarct size in man using 99m Tc polyphosphate.", "content": "We have critically evaluated the use of 99m Tc polyphosphate to estimate myocardial infarct size. Optimum conditions were first defined with respect to infarct edge definition, and relative activity over infarct and bone and in blood pool. The scintigrams of 53 patients with acute transmural myocardial infarction were recorded under these defined constant conditions and analysed in various ways. Visual grading of infarct area or intensity correlated poorly with other indices of infarct severity. Computer-assisted measurements of above-background infarct uptake area or of total farct activity correlated well with clinical, electrocardiographic, and enzymatic measurements of infarct severity.", "contents": "Estimation of myocardial infarct size in man using 99m Tc polyphosphate. We have critically evaluated the use of 99m Tc polyphosphate to estimate myocardial infarct size. Optimum conditions were first defined with respect to infarct edge definition, and relative activity over infarct and bone and in blood pool. The scintigrams of 53 patients with acute transmural myocardial infarction were recorded under these defined constant conditions and analysed in various ways. Visual grading of infarct area or intensity correlated poorly with other indices of infarct severity. Computer-assisted measurements of above-background infarct uptake area or of total farct activity correlated well with clinical, electrocardiographic, and enzymatic measurements of infarct severity."}
{"id": "PMID:465242", "title": "Uhl's anomaly.", "content": "Uhl's anomaly of the heart is a rare condition. Another well-documented case is presented with a review of the published reports outlining the main clinical features and the bad overall prognosis. Right atriotomy should be avoided if closure of the atrial septal defect is attempted.", "contents": "Uhl's anomaly. Uhl's anomaly of the heart is a rare condition. Another well-documented case is presented with a review of the published reports outlining the main clinical features and the bad overall prognosis. Right atriotomy should be avoided if closure of the atrial septal defect is attempted."}
{"id": "PMID:465243", "title": "Maximal supine exercise haemodynamics after open heart surgery for Fallot's tetralogy.", "content": "Maximal supine exercise studies at the time of heart catheterisation were performed one to five years after open heart surgery for Fallot's tetralogy on 29 subjects 6 to 16 years of age. During exercise right ventricular systolic pressure exceeded 50 mmHg in all but 2 subjects, and end-diastolic pressure increased to over 15 mmHg in 10 subjects. Pulmonary artery peak systolic pressure was abnormal in 5 patients. Maximal exercise cardiac index was below the normal range in only 2 subjects, but below the mean for normals in 80 per cent of the patients. Only 3 patients had clinical exercise performances below the 3rd centile of normal subjects using a maximal upright bicycle exercise test, and only 1 subject was below the normal range for endurance time on the Bruce treadmill test. The patients in this series performed better than those in other series, possibly because of their younger age at operation, the use of a large control series of normal subjects taken from a clinic population, the willingness of the patients to work to near exhaustion, and previous encouragement of the patients to become normally active children.", "contents": "Maximal supine exercise haemodynamics after open heart surgery for Fallot's tetralogy. Maximal supine exercise studies at the time of heart catheterisation were performed one to five years after open heart surgery for Fallot's tetralogy on 29 subjects 6 to 16 years of age. During exercise right ventricular systolic pressure exceeded 50 mmHg in all but 2 subjects, and end-diastolic pressure increased to over 15 mmHg in 10 subjects. Pulmonary artery peak systolic pressure was abnormal in 5 patients. Maximal exercise cardiac index was below the normal range in only 2 subjects, but below the mean for normals in 80 per cent of the patients. Only 3 patients had clinical exercise performances below the 3rd centile of normal subjects using a maximal upright bicycle exercise test, and only 1 subject was below the normal range for endurance time on the Bruce treadmill test. The patients in this series performed better than those in other series, possibly because of their younger age at operation, the use of a large control series of normal subjects taken from a clinic population, the willingness of the patients to work to near exhaustion, and previous encouragement of the patients to become normally active children."}
{"id": "PMID:465244", "title": "Long-term follow-up in primary pulmonary hypertension.", "content": "Two patients with severe primary pulmonary hypertension, who are alive 38 and 27 years after the onset of the disease, were closely followed by the authors for 24 years. No previous report of such a long course has been found. Despite pulmonary artery pressures at systemic level, effort syncope disappeared and there were long intervals without symptoms during which the 2 patients were able to take part in physical activity with minimal discomfort. Severe primary pulmonary hypertension should not always be considered a malignant disease.", "contents": "Long-term follow-up in primary pulmonary hypertension. Two patients with severe primary pulmonary hypertension, who are alive 38 and 27 years after the onset of the disease, were closely followed by the authors for 24 years. No previous report of such a long course has been found. Despite pulmonary artery pressures at systemic level, effort syncope disappeared and there were long intervals without symptoms during which the 2 patients were able to take part in physical activity with minimal discomfort. Severe primary pulmonary hypertension should not always be considered a malignant disease."}
{"id": "PMID:465245", "title": "Clinical electrophysiological effects of propranolol on normal sinus node function.", "content": "In 35 patients aged 18 to 69 years (mean 48) with clinical, electrocardiographic, or electrophysiological evidence of normal sinus node function, the effect of intravenous propranolol (0.1 mg/kg) was assessed on 3 indices of sinus node function. The drug significantly prolonged sinus node cycle length (12%), slightly prolonged the corrected sinus node recovery time (15%), and slightly but insignificantly lengthened sinuatrial conduction time. Propranolol may be administered safely in patients with normal sinus node function without the fear of producing severe sinus bradycardia, sinuatrial block, sinuatrial pauses, or prolonged sinus asystole, after spontaneous or stimulation-induced conversion of a tachycardia.", "contents": "Clinical electrophysiological effects of propranolol on normal sinus node function. In 35 patients aged 18 to 69 years (mean 48) with clinical, electrocardiographic, or electrophysiological evidence of normal sinus node function, the effect of intravenous propranolol (0.1 mg/kg) was assessed on 3 indices of sinus node function. The drug significantly prolonged sinus node cycle length (12%), slightly prolonged the corrected sinus node recovery time (15%), and slightly but insignificantly lengthened sinuatrial conduction time. Propranolol may be administered safely in patients with normal sinus node function without the fear of producing severe sinus bradycardia, sinuatrial block, sinuatrial pauses, or prolonged sinus asystole, after spontaneous or stimulation-induced conversion of a tachycardia."}
{"id": "PMID:465246", "title": "Comparability and reproducibility of apex cardiogram recorded with six different transducer systems.", "content": "A comparison was made in 7 dogs of the results obtained by 6 different apex cardiographic transducers applied before, during, and after controlled infusion of angiotensin and isoprenaline. The electrocardiogram, internal phonocardiogram, aortic and left ventricular pressure using a Telco micromanometer, and apex cardiogram were recorded simultaneously on magnetic tape and paper. Digital computer techniques were used to derive various measurements. The comparison of the 6 transducer systems was made expecially with respect to measurements derived from the normalised derivative, calculated using total as well as developed pressure or displacement. Measurements derived from left ventricular pressure were very reproducible. Differences in results of 'contractility' indices varied between 0.5 and 1.9 per cent. Indices from the apex cardiogram using 6 different transducer systems showed variations up to 20 per cent, with mean values varying between 3.2 and 8.1 per cent. There was a systematic deviation for one transducer system, which was responsible for a significant part of the observed variability. It may be concluded that in order to assure maximal reproducibility, technical characteristics of the apex cardiograph transducer should be taken into account and an optimal recording technique should be used.", "contents": "Comparability and reproducibility of apex cardiogram recorded with six different transducer systems. A comparison was made in 7 dogs of the results obtained by 6 different apex cardiographic transducers applied before, during, and after controlled infusion of angiotensin and isoprenaline. The electrocardiogram, internal phonocardiogram, aortic and left ventricular pressure using a Telco micromanometer, and apex cardiogram were recorded simultaneously on magnetic tape and paper. Digital computer techniques were used to derive various measurements. The comparison of the 6 transducer systems was made expecially with respect to measurements derived from the normalised derivative, calculated using total as well as developed pressure or displacement. Measurements derived from left ventricular pressure were very reproducible. Differences in results of 'contractility' indices varied between 0.5 and 1.9 per cent. Indices from the apex cardiogram using 6 different transducer systems showed variations up to 20 per cent, with mean values varying between 3.2 and 8.1 per cent. There was a systematic deviation for one transducer system, which was responsible for a significant part of the observed variability. It may be concluded that in order to assure maximal reproducibility, technical characteristics of the apex cardiograph transducer should be taken into account and an optimal recording technique should be used."}
{"id": "PMID:465247", "title": "A severe complication of pulmonary vein angiography.", "content": "A pulmonary vein angiogram was performed using a pressure injection with the tip of an end and side hole catheter in a near-wedge position; extravasation of contrast material into a bronchus occurred, causing severe bronchoconstriction. It may be possible to avoid this complication by the use of an end hole balloon catheter in the wedge position, slow flow rates of contrast material, and low pressure injections.", "contents": "A severe complication of pulmonary vein angiography. A pulmonary vein angiogram was performed using a pressure injection with the tip of an end and side hole catheter in a near-wedge position; extravasation of contrast material into a bronchus occurred, causing severe bronchoconstriction. It may be possible to avoid this complication by the use of an end hole balloon catheter in the wedge position, slow flow rates of contrast material, and low pressure injections."}
{"id": "PMID:465248", "title": "Mitral valve replacement for mitral stenosis caused by Libman-Sacks endocarditis.", "content": "A women who developed mitral stenosis from Libman-Sacks endocarditis is described. The mitral valve was replaced by a Starr-Edwards prosthesis. One year later, despite her being maintained on steroids and azathioprine, the verrucous endocarditis progressed to cause sudden, severe dysfunction of the prosthetic valve.", "contents": "Mitral valve replacement for mitral stenosis caused by Libman-Sacks endocarditis. A women who developed mitral stenosis from Libman-Sacks endocarditis is described. The mitral valve was replaced by a Starr-Edwards prosthesis. One year later, despite her being maintained on steroids and azathioprine, the verrucous endocarditis progressed to cause sudden, severe dysfunction of the prosthetic valve."}
{"id": "PMID:465249", "title": "Alternating atrial and ventricular tachycardia.", "content": "A patient with alternating atrial and ventricular tachycardia is described. He had an acute myocardial infarction and was taking digitalis. The atrial tachycardia, which was occasionally associated with aberrant ventricular conduction, was able to interrupt the ventricular tachycardia though the reverse was not true, suggesting a retrograde conduction block. This patient shows that atrial and ventricular arrhythmias can alternate and, since tachycardia with aberrant ventricular conduction can closely resemble ventricular tachycardia, intra-atrial electrocardiography may be necessary to establish an accurate diagnosis.", "contents": "Alternating atrial and ventricular tachycardia. A patient with alternating atrial and ventricular tachycardia is described. He had an acute myocardial infarction and was taking digitalis. The atrial tachycardia, which was occasionally associated with aberrant ventricular conduction, was able to interrupt the ventricular tachycardia though the reverse was not true, suggesting a retrograde conduction block. This patient shows that atrial and ventricular arrhythmias can alternate and, since tachycardia with aberrant ventricular conduction can closely resemble ventricular tachycardia, intra-atrial electrocardiography may be necessary to establish an accurate diagnosis."}
{"id": "PMID:465252", "title": "Cytotoxic effects of procaine, lignocaine and bupivacaine.", "content": "Cytotoxic effects of procaine, lignocaine and bupivacaine were investigated on cultures of Chinese hamster lung fibroblasts. Cell growth was inhibited over 24 h exposures with an ED50 of 0.17% for procaine, 0.07% for lignocaine and 0.02% for bupivacaine. Cell survival (measured as colony-forming ability) was reduced over 24 h exposure with an ED50 of 0.21% for procaine, 0.09% for lignocaine and 0.06% for bupivacaine. Morphological changes included vacuolation, cell rounding and retraction from the surface on which the cells were growing. Motility was reduced to about 50% of control by lignocaine 0.1%. On removal of the local anaesthetics, cell growth was resumed after about 15 h without evidence of chromosome damage. Corresponding changes induced by inhalation anaesthetics are only seen at two to three times the minimal alveolar concentration required for anaesthesia.", "contents": "Cytotoxic effects of procaine, lignocaine and bupivacaine. Cytotoxic effects of procaine, lignocaine and bupivacaine were investigated on cultures of Chinese hamster lung fibroblasts. Cell growth was inhibited over 24 h exposures with an ED50 of 0.17% for procaine, 0.07% for lignocaine and 0.02% for bupivacaine. Cell survival (measured as colony-forming ability) was reduced over 24 h exposure with an ED50 of 0.21% for procaine, 0.09% for lignocaine and 0.06% for bupivacaine. Morphological changes included vacuolation, cell rounding and retraction from the surface on which the cells were growing. Motility was reduced to about 50% of control by lignocaine 0.1%. On removal of the local anaesthetics, cell growth was resumed after about 15 h without evidence of chromosome damage. Corresponding changes induced by inhalation anaesthetics are only seen at two to three times the minimal alveolar concentration required for anaesthesia."}
{"id": "PMID:465253", "title": "Effect of the chronic administration of nitrous oxide 0.5% to gravid rats.", "content": "Twenty-four gravid Wistar rats (12 experimental and 12 control) were studied. From day 1 to day 19 of gestation the experimental group was exposed day and night to nitrous oxide 0.5%, while the control group was exposed to air. On day 19 the rats were killed and a detailed examination of the uterus, ovaries and fetuses was undertaken. The fetuses were fixed, cleared and stained with alizarin red, examined for skeletal anomalies and their crown-rump lengths measured. There was a significant decrease in litter size of the dams exposed to nitrous oxide as compared with control (P less than 0.001). Fetal resorption occurred in 10 of the 12 exposed dams. Skeletal anomalies were observed in 9% of the fetuses in the experimental group and there was an overall reduction in their size. None of the above changes were observed in the control.", "contents": "Effect of the chronic administration of nitrous oxide 0.5% to gravid rats. Twenty-four gravid Wistar rats (12 experimental and 12 control) were studied. From day 1 to day 19 of gestation the experimental group was exposed day and night to nitrous oxide 0.5%, while the control group was exposed to air. On day 19 the rats were killed and a detailed examination of the uterus, ovaries and fetuses was undertaken. The fetuses were fixed, cleared and stained with alizarin red, examined for skeletal anomalies and their crown-rump lengths measured. There was a significant decrease in litter size of the dams exposed to nitrous oxide as compared with control (P less than 0.001). Fetal resorption occurred in 10 of the 12 exposed dams. Skeletal anomalies were observed in 9% of the fetuses in the experimental group and there was an overall reduction in their size. None of the above changes were observed in the control."}
{"id": "PMID:465254", "title": "Effect of hypercarbia on hepatic blood flow and oxygen consumption in the greyhound.", "content": "The stability of a technique for measuring hepatic blood flow and oxygen consumption was established in six greyhounds anaesthetized with pentobarbitone (group A). Subsequently, the effects of increased PaCO2 were studied in another six animals (group B). With one exception (splenic blood flow) no significant changes were observed in any of the indices measured in group A. In group B, although hepatic arterial blood flow (HABF) decreased when PaCO2 increased, both portal venous blood flow (PVBF) and total liver blood flow (HABF+PVBF) increased. However, these changes were much less marked after 20 min. Hepatic oxygen consumption and splenic venous blood flow were unchanged with an increase in PaCO2.", "contents": "Effect of hypercarbia on hepatic blood flow and oxygen consumption in the greyhound. The stability of a technique for measuring hepatic blood flow and oxygen consumption was established in six greyhounds anaesthetized with pentobarbitone (group A). Subsequently, the effects of increased PaCO2 were studied in another six animals (group B). With one exception (splenic blood flow) no significant changes were observed in any of the indices measured in group A. In group B, although hepatic arterial blood flow (HABF) decreased when PaCO2 increased, both portal venous blood flow (PVBF) and total liver blood flow (HABF+PVBF) increased. However, these changes were much less marked after 20 min. Hepatic oxygen consumption and splenic venous blood flow were unchanged with an increase in PaCO2."}
{"id": "PMID:465255", "title": "Effect of hypotension induced with sodium nitroprusside on canine coronary arterial flow.", "content": "In seven dogs anaesthetized with pentobarbitone, coronary and pulmonary artery blood flows were measured using electromagnetic flow meters. The infusion of a 0.01% solution of sodium nitroprusside caused an initial small increase in mean coronary artery flow which returned to control as the arterial pressure decreased. No changes were noted in cardiac output nor were further changes observed in coronary flow. Heart rate was increased consistently during the hypotension and left ventricular dp/dt max was reduced as were coronary and total peripheral resistances. There were no significant changes in myocardial or total body oxygen extraction or consumption.", "contents": "Effect of hypotension induced with sodium nitroprusside on canine coronary arterial flow. In seven dogs anaesthetized with pentobarbitone, coronary and pulmonary artery blood flows were measured using electromagnetic flow meters. The infusion of a 0.01% solution of sodium nitroprusside caused an initial small increase in mean coronary artery flow which returned to control as the arterial pressure decreased. No changes were noted in cardiac output nor were further changes observed in coronary flow. Heart rate was increased consistently during the hypotension and left ventricular dp/dt max was reduced as were coronary and total peripheral resistances. There were no significant changes in myocardial or total body oxygen extraction or consumption."}
{"id": "PMID:465256", "title": "Depression of hypoxic pulmonary vasoconstriction in the dog by dopamine and isoprenaline.", "content": "The effects of dopamine and isoprenaline on the pulmonary vasoconstrictor response to alveolar hypoxia were assessed by measuring the redistribution of blood flow between the lungs in response to unilateral hypoxia. Dose rates of dopamine 25 micrograms kg-1 min-1 and isoprenaline 0.25 micrograms kg-1 min-1 (which produced equal increments in thecontractile force of the heart in dogs) produced a similar degree of depression of they hypoxic vasoconstrictor response, whereas dopamine 2.5 micrograms kg-1 min-1 had little effect on the response PaO2 during unilateral hypoxia was inversely related to the blood flow through the hypoxic lung.", "contents": "Depression of hypoxic pulmonary vasoconstriction in the dog by dopamine and isoprenaline. The effects of dopamine and isoprenaline on the pulmonary vasoconstrictor response to alveolar hypoxia were assessed by measuring the redistribution of blood flow between the lungs in response to unilateral hypoxia. Dose rates of dopamine 25 micrograms kg-1 min-1 and isoprenaline 0.25 micrograms kg-1 min-1 (which produced equal increments in thecontractile force of the heart in dogs) produced a similar degree of depression of they hypoxic vasoconstrictor response, whereas dopamine 2.5 micrograms kg-1 min-1 had little effect on the response PaO2 during unilateral hypoxia was inversely related to the blood flow through the hypoxic lung."}
{"id": "PMID:465257", "title": "Effect of etomidate on intracranial pressure and cerebral perfusion pressure.", "content": "Ten patients with intracranial lesions, anaesthetized with thiopentone and nitrous oxide (70%) in oxygen (30%) received etomidate 0.2 mg kg-1 i.v. Ventilation was controlled in each patient. Intracranial pressure (i.c.p.) and mean arterial pressure (m.a.p.) were recorded. I.c.p. decreased significantly in all patients (0.01 greater than P greater than 0.001). Although PaCO2 decreased during the period of measurement, the extent and time-course of this change suggested that it was not mainly responsible for changes in i.c.p. M.a.p. decreased in most patients, but the decrease was statistically significant only at 3 and 4 min after the administration of etomidate (0.05 greater than P greater than 0.02). The changes in cerebral perfusion pressure (c.p.p.) and heart rate were not clinically or statistically significant. We conclude that etomidate can be used for the induction of anaesthesia in patients with intracranial space-occupying lesions without increasing i.c.p. or seriously reducing c.p.p.", "contents": "Effect of etomidate on intracranial pressure and cerebral perfusion pressure. Ten patients with intracranial lesions, anaesthetized with thiopentone and nitrous oxide (70%) in oxygen (30%) received etomidate 0.2 mg kg-1 i.v. Ventilation was controlled in each patient. Intracranial pressure (i.c.p.) and mean arterial pressure (m.a.p.) were recorded. I.c.p. decreased significantly in all patients (0.01 greater than P greater than 0.001). Although PaCO2 decreased during the period of measurement, the extent and time-course of this change suggested that it was not mainly responsible for changes in i.c.p. M.a.p. decreased in most patients, but the decrease was statistically significant only at 3 and 4 min after the administration of etomidate (0.05 greater than P greater than 0.02). The changes in cerebral perfusion pressure (c.p.p.) and heart rate were not clinically or statistically significant. We conclude that etomidate can be used for the induction of anaesthesia in patients with intracranial space-occupying lesions without increasing i.c.p. or seriously reducing c.p.p."}
{"id": "PMID:465258", "title": "Presynaptic effect of I.V. anaesthetic agents at the neuromuscular junction.", "content": "The effect of Althesin, diazepam, ketamine, propanidid and thiopentone on the release of acetylcholine was tested at the mouse neuromuscular junction. Althesin, diazepam and thiopentone increased the quantal content of the end-plate potential. Ketamine at low concentration (3.6 micromol litre-1) had a similar effect, but at high concentration (116.7 micromol litre-1) quantal content decreased sharply. Propanidid and cremophor EL did not affect quantal content. The increase in quantal content antagonized the effect of postsynaptic depression on the amplitude of the end-plate potential. The lack of enhancement of acetylcholine release appears to explain the in vitro interaction of propanidid with tubocurarine. The diversity of presynaptic actions of these drugs makes it unlikely that this is an important mechanism in producing anaesthesia.", "contents": "Presynaptic effect of I.V. anaesthetic agents at the neuromuscular junction. The effect of Althesin, diazepam, ketamine, propanidid and thiopentone on the release of acetylcholine was tested at the mouse neuromuscular junction. Althesin, diazepam and thiopentone increased the quantal content of the end-plate potential. Ketamine at low concentration (3.6 micromol litre-1) had a similar effect, but at high concentration (116.7 micromol litre-1) quantal content decreased sharply. Propanidid and cremophor EL did not affect quantal content. The increase in quantal content antagonized the effect of postsynaptic depression on the amplitude of the end-plate potential. The lack of enhancement of acetylcholine release appears to explain the in vitro interaction of propanidid with tubocurarine. The diversity of presynaptic actions of these drugs makes it unlikely that this is an important mechanism in producing anaesthesia."}
{"id": "PMID:465259", "title": "Labetalol in controlled hypotension: administration of labetalol when adequate hypotension is difficult to achieve.", "content": "A study of controlled hypotension was undertaken in 50 major surgical patients using labetalol, a drug with both alpha- and beta-adrenoceptor blocking activity. The patients were such that difficulty in achieving controlled hypotension could be anticipated: the young, the anxious and those for whom halothane was contraindicated. The administration of labetalol quickly induced hypotension which was controlled easily and was rapidly antagonized.", "contents": "Labetalol in controlled hypotension: administration of labetalol when adequate hypotension is difficult to achieve. A study of controlled hypotension was undertaken in 50 major surgical patients using labetalol, a drug with both alpha- and beta-adrenoceptor blocking activity. The patients were such that difficulty in achieving controlled hypotension could be anticipated: the young, the anxious and those for whom halothane was contraindicated. The administration of labetalol quickly induced hypotension which was controlled easily and was rapidly antagonized."}
{"id": "PMID:465260", "title": "Extradural analgesia for caesarean section: a comparison with general anaesthesia.", "content": "Thirty-two patients received lumbar extradural anaesthesia for elective Caesarean section. The results obtained from this series were compared with those from a similar (control) group of parturients who received general anaesthesia. The extradural group (mothers and infants) were metabolically acidaemic relative to control. However, maternal to fetal blood-gas differences were comparable in the two series. Infants sustained respiration more rapidly after regional blockade, otherwise the clinical condition of the infants was similar with extradural and general anaesthesia. No advantage of conduction over general anaesthesia was evident from this study, with respect to the biochemical status of the infant at birth.", "contents": "Extradural analgesia for caesarean section: a comparison with general anaesthesia. Thirty-two patients received lumbar extradural anaesthesia for elective Caesarean section. The results obtained from this series were compared with those from a similar (control) group of parturients who received general anaesthesia. The extradural group (mothers and infants) were metabolically acidaemic relative to control. However, maternal to fetal blood-gas differences were comparable in the two series. Infants sustained respiration more rapidly after regional blockade, otherwise the clinical condition of the infants was similar with extradural and general anaesthesia. No advantage of conduction over general anaesthesia was evident from this study, with respect to the biochemical status of the infant at birth."}
{"id": "PMID:465261", "title": "Small endotracheal tubes: ventilator and intratracheal pressures during controlled ventilation.", "content": "The use of small endotracheal tubes reduces the trauma of intubation. Ventilator and tracheal pressures were measured during controlled ventilation with various tube dimensions and ventilation volumes. Ventilation with large volumes using small tracheal tubes results in high ventilator pressures. However, tracheal pressures are only marginally greater than those obtained with larger tubes. Small endotracheal tubes and high ventilation volumes result in a positive tracheal pressure at the end of expiration. The measured end-expiratory pressures are within the limits which might be used therapeutically (in PEEP). The force required to reshape endotracheal tubes of varvious dimensions to an \"anatomical\" shape was related to the tube dimensions; the beneficial effects of performed, \"anatomically shaped\" endotracheal tubes can be achieved by using small tubes of standard design.", "contents": "Small endotracheal tubes: ventilator and intratracheal pressures during controlled ventilation. The use of small endotracheal tubes reduces the trauma of intubation. Ventilator and tracheal pressures were measured during controlled ventilation with various tube dimensions and ventilation volumes. Ventilation with large volumes using small tracheal tubes results in high ventilator pressures. However, tracheal pressures are only marginally greater than those obtained with larger tubes. Small endotracheal tubes and high ventilation volumes result in a positive tracheal pressure at the end of expiration. The measured end-expiratory pressures are within the limits which might be used therapeutically (in PEEP). The force required to reshape endotracheal tubes of varvious dimensions to an \"anatomical\" shape was related to the tube dimensions; the beneficial effects of performed, \"anatomically shaped\" endotracheal tubes can be achieved by using small tubes of standard design."}
{"id": "PMID:465266", "title": "Airway closure and fluid filtration in the lung.", "content": "We have investigated the effect of airway closure on fluid balance in the isolated, perfused rabbit lung in which the alveolar, pleural and vascular pressures could be regulated independently. The rate of filtration into the interstitial space was monitored by continuous weighing. A reduction in pleural pressure of 0.5 kPa with open airways increased fluid filtration by 0.11 g min-1. Reduction in pleural pressure of 0.5 kPa with the main airways closed reduced the alveolar pressure by 0.4 kPa and increased fluid filtration by 0.86 g minp1. We concluded that the pleural pressure is transmitted poorly to the alveolar interstitial space in the normal lung with open airways. However, airway closure allows the pleural pressure to be transmitted almost completely to this interstitium, with interstitial oedema as a possible consequence.", "contents": "Airway closure and fluid filtration in the lung. We have investigated the effect of airway closure on fluid balance in the isolated, perfused rabbit lung in which the alveolar, pleural and vascular pressures could be regulated independently. The rate of filtration into the interstitial space was monitored by continuous weighing. A reduction in pleural pressure of 0.5 kPa with open airways increased fluid filtration by 0.11 g min-1. Reduction in pleural pressure of 0.5 kPa with the main airways closed reduced the alveolar pressure by 0.4 kPa and increased fluid filtration by 0.86 g minp1. We concluded that the pleural pressure is transmitted poorly to the alveolar interstitial space in the normal lung with open airways. However, airway closure allows the pleural pressure to be transmitted almost completely to this interstitium, with interstitial oedema as a possible consequence."}
{"id": "PMID:465267", "title": "Effect of i.v. administration of lignocaine and tetrodotoxin on sensory units in the tooth of the cat.", "content": "A study in cats was designed to investigate the blocking of sensory nerve terminals by i.v. injection of lipid-soluble local anaesthetics such as lignocaine. I.v. injection of lignocaine 15--30 mg kg-1 did not completely block sensory nerve endings in the tooth of the cat, whilst tetrodotoxin (TTX) in large non-toxic doses did so. Based on the i.v. doses of TTX and the relationship between the local anaesthetic potency of the two substances when injected intra-arterially, it was calculated that lethal doses of i.v. administered lignocaine are necessary to block sensory nerve endings. It seems unlikely that peripheral pain pathways can be blocked by systemic i.v. injections of local anaesthetics.", "contents": "Effect of i.v. administration of lignocaine and tetrodotoxin on sensory units in the tooth of the cat. A study in cats was designed to investigate the blocking of sensory nerve terminals by i.v. injection of lipid-soluble local anaesthetics such as lignocaine. I.v. injection of lignocaine 15--30 mg kg-1 did not completely block sensory nerve endings in the tooth of the cat, whilst tetrodotoxin (TTX) in large non-toxic doses did so. Based on the i.v. doses of TTX and the relationship between the local anaesthetic potency of the two substances when injected intra-arterially, it was calculated that lethal doses of i.v. administered lignocaine are necessary to block sensory nerve endings. It seems unlikely that peripheral pain pathways can be blocked by systemic i.v. injections of local anaesthetics."}
{"id": "PMID:465268", "title": "Pattern of respiration and responses to carbon dioxide during trichloroethylene anaesthesia in the cat.", "content": "Tidal volume (VT), minute ventilation (VE), the duration of inspiratats anaesthetized with 0.7%, 1% and 1.5% (inspired) trichloroethylene (TCE). The tracheal cannula was occluded at intervals at the start of inspiration and the tracheal pressure was measured to assess the force of contraction of the respiratory muscles. Anaesthesia with TCE 0.7% was associated with an increase in VE, a reduction in VT, and a marked increase in respiratory frequency and mean inspiratory flow rate, but PaCO2 values did not differ significantly from those in conscious animals. Ventilation was also greater than in conscious animals during anaesthesia with TCE 1%. TCE 1.5% caused a significantly greater PaCO2 than in conscious animals. All concentrations of TCE caused a reduction in the ventilatory response to carbon dioxide, measured by the steady-state method. Cervical vagal section did not abolish the tachypnoea caused by TCE.", "contents": "Pattern of respiration and responses to carbon dioxide during trichloroethylene anaesthesia in the cat. Tidal volume (VT), minute ventilation (VE), the duration of inspiratats anaesthetized with 0.7%, 1% and 1.5% (inspired) trichloroethylene (TCE). The tracheal cannula was occluded at intervals at the start of inspiration and the tracheal pressure was measured to assess the force of contraction of the respiratory muscles. Anaesthesia with TCE 0.7% was associated with an increase in VE, a reduction in VT, and a marked increase in respiratory frequency and mean inspiratory flow rate, but PaCO2 values did not differ significantly from those in conscious animals. Ventilation was also greater than in conscious animals during anaesthesia with TCE 1%. TCE 1.5% caused a significantly greater PaCO2 than in conscious animals. All concentrations of TCE caused a reduction in the ventilatory response to carbon dioxide, measured by the steady-state method. Cervical vagal section did not abolish the tachypnoea caused by TCE."}
{"id": "PMID:465269", "title": "Haemodynamic consequences of citrate infusion in the anaesthetized dog: comparison between two citrate solutions and the influence of beta blockade.", "content": "We have compared the effects of a solution of acid--citrate--dextrose (ACD) with those of a solution of citrate--phosphate--dextrose (CPD), infused at equal rates, on blood calcium ion concentration and different indices of haemodynamic performance in 17 dogs. The influence of beta adrenergic blockade on these changes was examined. The effects of ACD and CPD were studied in five dogs and were similar. Peripheral vascular changes were the principal cause of arterial hypotension. In six dogs, propranolol 0.5 mg kg-1 intensified the hypocalcaemia-induced left ventricular dysfunction.", "contents": "Haemodynamic consequences of citrate infusion in the anaesthetized dog: comparison between two citrate solutions and the influence of beta blockade. We have compared the effects of a solution of acid--citrate--dextrose (ACD) with those of a solution of citrate--phosphate--dextrose (CPD), infused at equal rates, on blood calcium ion concentration and different indices of haemodynamic performance in 17 dogs. The influence of beta adrenergic blockade on these changes was examined. The effects of ACD and CPD were studied in five dogs and were similar. Peripheral vascular changes were the principal cause of arterial hypotension. In six dogs, propranolol 0.5 mg kg-1 intensified the hypocalcaemia-induced left ventricular dysfunction."}
{"id": "PMID:465270", "title": "Experience with spinal analgesia in a British obstetric unit.", "content": "A series of 443 spinal anaesthetics is described. The procedures included operative vaginal delivery, removal of retained placenta and a miscellaneous group common to most obstetric units. Failure to provide effective relief of pain occurred in 5.2% of patients. There was one potentially serious complication, but with this exception hypotension was not a feature. Headache following spinal analgesia was experienced by 16.3% of patients, the frequency being greater among those who received spinal analgesia at or shortly after delivery, but was unrelated to the size of the needle (23-gauge of 25-gauge) used. Extradural blood patch promptly and permanently cured the headache. The duration of sensory and motor loss after operation varied considerably with the local anesthetic agent used. The re-introduction of spinal analgesia into British obstetric anaesthetic practice is advocated.", "contents": "Experience with spinal analgesia in a British obstetric unit. A series of 443 spinal anaesthetics is described. The procedures included operative vaginal delivery, removal of retained placenta and a miscellaneous group common to most obstetric units. Failure to provide effective relief of pain occurred in 5.2% of patients. There was one potentially serious complication, but with this exception hypotension was not a feature. Headache following spinal analgesia was experienced by 16.3% of patients, the frequency being greater among those who received spinal analgesia at or shortly after delivery, but was unrelated to the size of the needle (23-gauge of 25-gauge) used. Extradural blood patch promptly and permanently cured the headache. The duration of sensory and motor loss after operation varied considerably with the local anesthetic agent used. The re-introduction of spinal analgesia into British obstetric anaesthetic practice is advocated."}
{"id": "PMID:465271", "title": "Diazepam and droperidol as i.v. premedicants.", "content": "The effects of i.v. diazepam and droperidol both alone and in combination administered as premedication were studied in 240 patients. Relief of anxiety, sedation, lack of recall, acceptance by both patient and physician and side-effects were evaluated. Overall, the combination of droperidol 2.5 mg with diazepam 5 mg produced better ratings of these variables than could be achieved with either droperidol 10 mg or diazepam 10 mg alone. Larger doses of droperidol with diazepam produce an increased frequency of anxiety; larger doses of diazepam with droperidol may cause over-sedation.", "contents": "Diazepam and droperidol as i.v. premedicants. The effects of i.v. diazepam and droperidol both alone and in combination administered as premedication were studied in 240 patients. Relief of anxiety, sedation, lack of recall, acceptance by both patient and physician and side-effects were evaluated. Overall, the combination of droperidol 2.5 mg with diazepam 5 mg produced better ratings of these variables than could be achieved with either droperidol 10 mg or diazepam 10 mg alone. Larger doses of droperidol with diazepam produce an increased frequency of anxiety; larger doses of diazepam with droperidol may cause over-sedation."}
{"id": "PMID:465273", "title": "Cardiorespiratory changes following induction of anaesthesia with etomidate in patients with cardiac disease.", "content": "The cardiorespiratory effects of etomidate were measured in two groups of six patients with aortic or mitral valve disease. The induction of anaesthesia with etomidate 0.3 mg kg-1 was followed by a second dose 10 min later. After the first dose, a 19% decrease in systemic arterial pressure was associated with a decrease in systemic vascular resistance and left ventricular heart work. Cardiac index, pulmonary artery pressure and wedge pressure all decreased slightly. Central venous pressure and heart rate did not change. A slight increase in respiratory frequency failed to prevent an increase in PACO2. Changes after the second dose were similar. The two groups of patients did not differ significantly in their response to etomidate. No patient complained of pain during injection, nor did myoclonic movements occur.", "contents": "Cardiorespiratory changes following induction of anaesthesia with etomidate in patients with cardiac disease. The cardiorespiratory effects of etomidate were measured in two groups of six patients with aortic or mitral valve disease. The induction of anaesthesia with etomidate 0.3 mg kg-1 was followed by a second dose 10 min later. After the first dose, a 19% decrease in systemic arterial pressure was associated with a decrease in systemic vascular resistance and left ventricular heart work. Cardiac index, pulmonary artery pressure and wedge pressure all decreased slightly. Central venous pressure and heart rate did not change. A slight increase in respiratory frequency failed to prevent an increase in PACO2. Changes after the second dose were similar. The two groups of patients did not differ significantly in their response to etomidate. No patient complained of pain during injection, nor did myoclonic movements occur."}
{"id": "PMID:465276", "title": "Absorption and excretion of rapid and slow release oxprenolol and their effects on heart rate and blood pressure during exercise.", "content": "1. Plasma concentrations and heart rate and blood pressure effects of 160 mg oxprenolol as standard rapid release (RR) and slow release (SR) tablets were compared in healthy volunteers. Peak plasma concentrations were lower with SR tablets than with RR tablets and the peak was delayed. 2. Absorption of oxprenolol was described adequately by first order kinetics with both preparations. The apparent half-life of absorption was 0.40 h with RR and 2.4 h for the SR formulation. The apparent elimination half-life of oxprenolol was about 2 h. Relative bioavailabilities of the two formulations were similar. 3. The effectiveness of oxprenolol RR and SR were assessed by their effects on heart rate in severe exercise (EHR) and also by their effects on blood pressure at rest and during exercise. 4. Maximum reductions in these variables coincided with peak oxprenolol concentrations. The effects on EHR and blood pressure parameters had a distinct time course but there was no difference between the time course of inhibition of each variable for the two formulations over 24 h.", "contents": "Absorption and excretion of rapid and slow release oxprenolol and their effects on heart rate and blood pressure during exercise. 1. Plasma concentrations and heart rate and blood pressure effects of 160 mg oxprenolol as standard rapid release (RR) and slow release (SR) tablets were compared in healthy volunteers. Peak plasma concentrations were lower with SR tablets than with RR tablets and the peak was delayed. 2. Absorption of oxprenolol was described adequately by first order kinetics with both preparations. The apparent half-life of absorption was 0.40 h with RR and 2.4 h for the SR formulation. The apparent elimination half-life of oxprenolol was about 2 h. Relative bioavailabilities of the two formulations were similar. 3. The effectiveness of oxprenolol RR and SR were assessed by their effects on heart rate in severe exercise (EHR) and also by their effects on blood pressure at rest and during exercise. 4. Maximum reductions in these variables coincided with peak oxprenolol concentrations. The effects on EHR and blood pressure parameters had a distinct time course but there was no difference between the time course of inhibition of each variable for the two formulations over 24 h."}
{"id": "PMID:465277", "title": "Bioavailability and disposition of metoprolol and hydrochlorothiazide combined in one tablet and of separate doses of hydrochlorothiazide.", "content": "1. The plasma levels and the urinary excretion of hydrochlorothiazide (HCT) have been studied after administration of single doses of 12.5 and 25 mg of the drug in solution and in combination with 100 mg of the selective beta 1-adrenoreceptor antagonist metoprolol in a rapidly dissolving tablet. 2. Metoprolol did not significantly influence the bioavailability or the time-course of HCT. 3. HCT had no significant effect on the time-course or the plasma levels of metoprolol. The average half-life, 4.4 +/- 0.9 h, is about the same as previously observed for separate doses of this drug. 4. It seems unlikely that repeated doses of the combination product studied will lead to biopharmaceutic or pharmacokinetic interactions of clinical importance.", "contents": "Bioavailability and disposition of metoprolol and hydrochlorothiazide combined in one tablet and of separate doses of hydrochlorothiazide. 1. The plasma levels and the urinary excretion of hydrochlorothiazide (HCT) have been studied after administration of single doses of 12.5 and 25 mg of the drug in solution and in combination with 100 mg of the selective beta 1-adrenoreceptor antagonist metoprolol in a rapidly dissolving tablet. 2. Metoprolol did not significantly influence the bioavailability or the time-course of HCT. 3. HCT had no significant effect on the time-course or the plasma levels of metoprolol. The average half-life, 4.4 +/- 0.9 h, is about the same as previously observed for separate doses of this drug. 4. It seems unlikely that repeated doses of the combination product studied will lead to biopharmaceutic or pharmacokinetic interactions of clinical importance."}
{"id": "PMID:465278", "title": "Pharmacokinetics, pharmacology of atenolol and effect of renal disease.", "content": "1. The pharmacokinetics of intravenous and oral atenolol (50 mg) in six healthy volunteers was studied. Plasma, saliva and urine were collected up to 24 h after each dose. 2. There was no significant difference in atenolol half-life when administered by the two routes. Bioavailability of the orally administered atenolol was 50%. 3. Atenolol levels in saliva required about 2 h to reach equilibrium with plasma drug levels. 4. A comparison between the pharmacokinetics and pharmacology of atenolol was made in twelve healthy subjects. 5. Dose-independent pharmacokinetics were observed. Reductions in resting heart rate and arterial blood pressure were proportional to either the logarithm of dose or area under the plasma concentration time curve or cumulative urinary atenolol excretion. 6. Plasma elimination half-life in five subjects with renal failure was prolonged.", "contents": "Pharmacokinetics, pharmacology of atenolol and effect of renal disease. 1. The pharmacokinetics of intravenous and oral atenolol (50 mg) in six healthy volunteers was studied. Plasma, saliva and urine were collected up to 24 h after each dose. 2. There was no significant difference in atenolol half-life when administered by the two routes. Bioavailability of the orally administered atenolol was 50%. 3. Atenolol levels in saliva required about 2 h to reach equilibrium with plasma drug levels. 4. A comparison between the pharmacokinetics and pharmacology of atenolol was made in twelve healthy subjects. 5. Dose-independent pharmacokinetics were observed. Reductions in resting heart rate and arterial blood pressure were proportional to either the logarithm of dose or area under the plasma concentration time curve or cumulative urinary atenolol excretion. 6. Plasma elimination half-life in five subjects with renal failure was prolonged."}
{"id": "PMID:465279", "title": "Interaction of atenolol and adrenaline with respect to intraocular pressure.", "content": "1. The effects of topically administered atenolol 4% and adrenaline 1% on intraocular pressure (IOP) were investigated in fourteen healthy male human volunteers. Both the sole and combined effects of single doses of the formulations were recorded over a 7 h period. 2. Adrenaline and atenolol both produced significant reductions in IOP when compared to the placebo control, although the pattern of response differed. 3. Adrenaline had a rapid onset of action, resulting in a significant pressure reduction by 45 min post drug administration. A small upswing in IOP then occurred between 45 and 90 min, followed by a prolonged secondary reduction phase. 4. Atenolol produced a gradual reduction in IOP which reached a maximum at 180 min post drug administration and subsequently declined between 300 and 420 min. 5. Combined treatment with atenolol and adrenaline resulted in additive effects up to 180 min, and the early pressure upswing recorded with adrenaline was completely abolished. 6. The results are compatible with the hypothesis that beta 1-adrenoceptor stimulation elevates intraocular pressure. The correlation of these results with relevant animal and human pharmacological data is discussed.", "contents": "Interaction of atenolol and adrenaline with respect to intraocular pressure. 1. The effects of topically administered atenolol 4% and adrenaline 1% on intraocular pressure (IOP) were investigated in fourteen healthy male human volunteers. Both the sole and combined effects of single doses of the formulations were recorded over a 7 h period. 2. Adrenaline and atenolol both produced significant reductions in IOP when compared to the placebo control, although the pattern of response differed. 3. Adrenaline had a rapid onset of action, resulting in a significant pressure reduction by 45 min post drug administration. A small upswing in IOP then occurred between 45 and 90 min, followed by a prolonged secondary reduction phase. 4. Atenolol produced a gradual reduction in IOP which reached a maximum at 180 min post drug administration and subsequently declined between 300 and 420 min. 5. Combined treatment with atenolol and adrenaline resulted in additive effects up to 180 min, and the early pressure upswing recorded with adrenaline was completely abolished. 6. The results are compatible with the hypothesis that beta 1-adrenoceptor stimulation elevates intraocular pressure. The correlation of these results with relevant animal and human pharmacological data is discussed."}
{"id": "PMID:465280", "title": "Pharmacokinetics of hydrochlorothiazide in patients with congestive heart failure.", "content": "1. Hydrochlorothiazide (HCT, 50-75 mg) was administered orally to seven patients with cardiac failure. 2. Plasma levels and urinary concentration of HCT were determined by GLC. 3. The gastrointestinal uptake of the diuretic in three patients was reduced to approximately half that seen in healthy controls. 4. Plasma halflife of HCT was correlated with endogenous creatinine clearance. 5. Pharmacokinetics of HCT are considerably changed in cardiac failure.", "contents": "Pharmacokinetics of hydrochlorothiazide in patients with congestive heart failure. 1. Hydrochlorothiazide (HCT, 50-75 mg) was administered orally to seven patients with cardiac failure. 2. Plasma levels and urinary concentration of HCT were determined by GLC. 3. The gastrointestinal uptake of the diuretic in three patients was reduced to approximately half that seen in healthy controls. 4. Plasma halflife of HCT was correlated with endogenous creatinine clearance. 5. Pharmacokinetics of HCT are considerably changed in cardiac failure."}
{"id": "PMID:465281", "title": "Effects of aspirin and dimenhydrinate (Dramamine) on visual processes.", "content": "1. The effect of three doses of either aspirin (1280 mg each) or dimenhydrinate (Dramamine) (100 mg each) in an 8 h period on visual processes was investigated double-blind on sixteen volunteer subjects. 2. Tests included night-vision, colour-discrimination, stereoacuity and stereopsis, reaction-time, pupil size, eye-movements, visual evoked cortical potentials, electroencephalographs, a dial-reading task, and a test of texture discrimination. 3. Both drugs affected colour discrimination, and dimenhydrinate degraded night-vision, reaction-time, and stereopsis. 4. The effect of aspirin was minor, but the impairments produced by dimenhydrinate appear to be of practical importance.", "contents": "Effects of aspirin and dimenhydrinate (Dramamine) on visual processes. 1. The effect of three doses of either aspirin (1280 mg each) or dimenhydrinate (Dramamine) (100 mg each) in an 8 h period on visual processes was investigated double-blind on sixteen volunteer subjects. 2. Tests included night-vision, colour-discrimination, stereoacuity and stereopsis, reaction-time, pupil size, eye-movements, visual evoked cortical potentials, electroencephalographs, a dial-reading task, and a test of texture discrimination. 3. Both drugs affected colour discrimination, and dimenhydrinate degraded night-vision, reaction-time, and stereopsis. 4. The effect of aspirin was minor, but the impairments produced by dimenhydrinate appear to be of practical importance."}
{"id": "PMID:465282", "title": "N-Methyl-benzylamine, a metabolite of pargyline in man.", "content": "1. N-methyl-benzylamine was identified in human urine and plasma after the administration of pargyline in man. This metabolite was identified by thin layer chromatography mobility, gas chromatographic retention time and mass spectrum relative to authentic N-methyl-benzylamine. 2. [14C]-N-methyl-benzylamine, together with a small amount of [14C]-benzylamine, was formed in vitro from [14C]-pargyline when this was incubated with human and rat liver microsomes. 3. The urinary excretion rates of pargyline and its metabolite have also been described.", "contents": "N-Methyl-benzylamine, a metabolite of pargyline in man. 1. N-methyl-benzylamine was identified in human urine and plasma after the administration of pargyline in man. This metabolite was identified by thin layer chromatography mobility, gas chromatographic retention time and mass spectrum relative to authentic N-methyl-benzylamine. 2. [14C]-N-methyl-benzylamine, together with a small amount of [14C]-benzylamine, was formed in vitro from [14C]-pargyline when this was incubated with human and rat liver microsomes. 3. The urinary excretion rates of pargyline and its metabolite have also been described."}
{"id": "PMID:465283", "title": "Plasma levels of 5-fluorouracil after oral and intravenous administration in cancer patients.", "content": "1. Plasma levels of 5-fluorouracil (5FU) have been determined in eleven cancer patients after 0.5 g and 1.0 g intravenous doses, and in one patient after paired 1.0 g oral and intravenous doses. 2. The plasma half-life after the 0.5 g intravenous dose was relatively constant, irrespective of the stage and spread of the disease. 3. Plasma kinetics of the drug were dose dependent. Doubling of the intravenous dose produced a 1.5-fold increase in plasma half life, a two-fold increase in initial plasma drug concentration, and a three-fold increase in area under the concentration/time curve. 4. In one patient receiving paired 1.0 g intravenous and oral doses nine weeks apart, an increase in the bioavailability of the drug coincided with a marked clinical regression in palpable intra-abdominal metastases. 5. The significance of measuring plasma drug kinetics and their relationship to drug efficacy and toxicity are discussed.", "contents": "Plasma levels of 5-fluorouracil after oral and intravenous administration in cancer patients. 1. Plasma levels of 5-fluorouracil (5FU) have been determined in eleven cancer patients after 0.5 g and 1.0 g intravenous doses, and in one patient after paired 1.0 g oral and intravenous doses. 2. The plasma half-life after the 0.5 g intravenous dose was relatively constant, irrespective of the stage and spread of the disease. 3. Plasma kinetics of the drug were dose dependent. Doubling of the intravenous dose produced a 1.5-fold increase in plasma half life, a two-fold increase in initial plasma drug concentration, and a three-fold increase in area under the concentration/time curve. 4. In one patient receiving paired 1.0 g intravenous and oral doses nine weeks apart, an increase in the bioavailability of the drug coincided with a marked clinical regression in palpable intra-abdominal metastases. 5. The significance of measuring plasma drug kinetics and their relationship to drug efficacy and toxicity are discussed."}
{"id": "PMID:465290", "title": "Influence of various antihypertensive agents on lifespan of renal hypertensive rats.", "content": "1 Daily treatment of two-kidney clipped renal hypertensive rats with hydrallazine, hydrochlorothiazide (HCTZ) and a new orally active inhibitor of the angiotensin-converting enzyme, captopril (SQ14,225), was correlated with survival rates for up to 9 months. 2 The groups of rats given captopril alone or captopril plus intermittent or chronic HCTZ had the best survival rate, whereas HCTZ alone or hydrallazine did not benefically affect survival. 3 Survival rates correlated well with control of BP in these animals.", "contents": "Influence of various antihypertensive agents on lifespan of renal hypertensive rats. 1 Daily treatment of two-kidney clipped renal hypertensive rats with hydrallazine, hydrochlorothiazide (HCTZ) and a new orally active inhibitor of the angiotensin-converting enzyme, captopril (SQ14,225), was correlated with survival rates for up to 9 months. 2 The groups of rats given captopril alone or captopril plus intermittent or chronic HCTZ had the best survival rate, whereas HCTZ alone or hydrallazine did not benefically affect survival. 3 Survival rates correlated well with control of BP in these animals."}
{"id": "PMID:465291", "title": "Human pharmacology of narcotic antagonists.", "content": "1 Human studies at the Addiction Research Center enable narcotic antagonists to be classified into three subgroups: (1) nalorphine-like agents; (2) pure antagonists; and (3) morphine-like agents. 2 Six narcotic antagonists (pentazocine, nalbuphine, cyclazocine, butorphanol, propiram and buprenorphine) developed in recent years seem to have a lesser abuse potential than codeine or propoxyphene. 3 When adjusted for relative availability of the agents, epidemiological data shows that pentazocine is abused less than codeine or propoxyphene in the US. 4 Recent studies with buprenorphine indicate that this agent would find application both as an analgesic of low abuse potential and as a new type of drug for the treatment of addiction.", "contents": "Human pharmacology of narcotic antagonists. 1 Human studies at the Addiction Research Center enable narcotic antagonists to be classified into three subgroups: (1) nalorphine-like agents; (2) pure antagonists; and (3) morphine-like agents. 2 Six narcotic antagonists (pentazocine, nalbuphine, cyclazocine, butorphanol, propiram and buprenorphine) developed in recent years seem to have a lesser abuse potential than codeine or propoxyphene. 3 When adjusted for relative availability of the agents, epidemiological data shows that pentazocine is abused less than codeine or propoxyphene in the US. 4 Recent studies with buprenorphine indicate that this agent would find application both as an analgesic of low abuse potential and as a new type of drug for the treatment of addiction."}
{"id": "PMID:465292", "title": "Pharmacological and clinical importance of narcotic antagonists and mixed antagonists--use in cardiology.", "content": "1 The treatment of pain of cardiac origin requires a knowledge of the haemodynamic action of the analgesic agents used. 2 The haemodynamic effects of morphine, diamorphine, pavaveretum, pethidine and pentazocine are reviewed. 3 Clinical experience with the new antagonist analgesic buprenorphine is reported. 4 These studies indicate that buprenorphine may be the agent of choice for the relief of severe pain in patients with unstable circulation.", "contents": "Pharmacological and clinical importance of narcotic antagonists and mixed antagonists--use in cardiology. 1 The treatment of pain of cardiac origin requires a knowledge of the haemodynamic action of the analgesic agents used. 2 The haemodynamic effects of morphine, diamorphine, pavaveretum, pethidine and pentazocine are reviewed. 3 Clinical experience with the new antagonist analgesic buprenorphine is reported. 4 These studies indicate that buprenorphine may be the agent of choice for the relief of severe pain in patients with unstable circulation."}
{"id": "PMID:465293", "title": "Use of narcotic antagonists in anaesthesia.", "content": "1 The introduction of morphine antagonists into anaesthesiology has aroused great scientific interest, increased our knowledge of analgesia, and opened new frontiers for therapeutics. 2 The advantages and disadvantages of these compounds are described and assessed. 3 The best choices for treatment of pain are the most potent and long-acting drugs of this group with a wide safety margin. As an antidote to narcotic agonists, naloxone seems to be the most suitable drug in the majority of cases, although in certain conditions drugs having less rapid onset of action and of longer duration may be more desirable. 4 In all cases of treatment after anaesthesia when antagonists to narcotic analgesics are used, drug administration must depend on careful control, which necessitates specific and prolonged patient care by specifically trained and competent staff.", "contents": "Use of narcotic antagonists in anaesthesia. 1 The introduction of morphine antagonists into anaesthesiology has aroused great scientific interest, increased our knowledge of analgesia, and opened new frontiers for therapeutics. 2 The advantages and disadvantages of these compounds are described and assessed. 3 The best choices for treatment of pain are the most potent and long-acting drugs of this group with a wide safety margin. As an antidote to narcotic agonists, naloxone seems to be the most suitable drug in the majority of cases, although in certain conditions drugs having less rapid onset of action and of longer duration may be more desirable. 4 In all cases of treatment after anaesthesia when antagonists to narcotic analgesics are used, drug administration must depend on careful control, which necessitates specific and prolonged patient care by specifically trained and competent staff."}
{"id": "PMID:465294", "title": "Side-effects of mixed agonist-antagonist analgesics used in sequential anaesthesia.", "content": "1 Mixed agonist-antagonist analgesics have analgesic action but also possess a range of side-effects. 2 Narcotic antagonists do not reverse the non-specific effects of opiates. 3 Under certain circumstances the effects of agonists and mixed agonist-antagonists can be additive. 4 Chronic dosage of mixed agonist-antagonists leads to a lower level of dependence than that observed with the standard narcotics. 5 Mixed agonist-antagonists may not antagonize the respiratory effects of narcotics and may result in potentiation of such depression.", "contents": "Side-effects of mixed agonist-antagonist analgesics used in sequential anaesthesia. 1 Mixed agonist-antagonist analgesics have analgesic action but also possess a range of side-effects. 2 Narcotic antagonists do not reverse the non-specific effects of opiates. 3 Under certain circumstances the effects of agonists and mixed agonist-antagonists can be additive. 4 Chronic dosage of mixed agonist-antagonists leads to a lower level of dependence than that observed with the standard narcotics. 5 Mixed agonist-antagonists may not antagonize the respiratory effects of narcotics and may result in potentiation of such depression."}
{"id": "PMID:465295", "title": "Purification of a protein associated with human bronchogenic squamous-cell carcinoma.", "content": "A heteroantiserum raised in rabbits to extracts of human squamous-cell carcinoma of the lung which exhibited marked tumour specificity was used to monitor the fractionation and isolation of a tumour-associated component of the extract. KC1 extracts of pools of both normal lung and bronchogenic squamous-cell carcinoma were subjected to a series of purification steps involving acid precipitation, salting out, DEAE chromatography and preparative polyacrylamide-gel electrophoresis. At each stage, fractions were tested for their ability to react in the complement-fixation assay with the antiserum. A protein was ultimately isolated  which did not appear to be present at detectable levels in an equivalent fraction of normal lung extract, reacted with the heteroantiserum, and appeared to be present in all extracts of squamous-cell carcinoma.", "contents": "Purification of a protein associated with human bronchogenic squamous-cell carcinoma. A heteroantiserum raised in rabbits to extracts of human squamous-cell carcinoma of the lung which exhibited marked tumour specificity was used to monitor the fractionation and isolation of a tumour-associated component of the extract. KC1 extracts of pools of both normal lung and bronchogenic squamous-cell carcinoma were subjected to a series of purification steps involving acid precipitation, salting out, DEAE chromatography and preparative polyacrylamide-gel electrophoresis. At each stage, fractions were tested for their ability to react in the complement-fixation assay with the antiserum. A protein was ultimately isolated  which did not appear to be present at detectable levels in an equivalent fraction of normal lung extract, reacted with the heteroantiserum, and appeared to be present in all extracts of squamous-cell carcinoma."}
{"id": "PMID:465296", "title": "Effect of carrageenan on the non-specific resistance of mice to injected syngeneic tumour cells, alone or in mixtures.", "content": "The mechanisms of non-specific resistance to syngeneic methylcholanthrene-induced fibrosarcomas of mice were investigated. Results showed that a small tumour graft of 0.05 X 10(5) cells is greatly enhanced in growth when admixed with large numbers of cell fragments, killed cells or viable non-replicating cells. The enhancement of small tumour grafts in cell mixtures was found to be non-specific. Carrageenan, a known anti-macrophage agent, significantly increased tumour growth in normal mice. However, it did not enhance the increased tumour growth of 0.05 X 10(5) cells mixed with 10(6) viable, non-replicating mitomycin C-treated tumour cells. The latter observation indicates that carrageenan and admixed cells interfere with the same tumour-inhibitory mechanism and therefore cannot produce additive effects. The results give support to the concept of a non-specific macrophage \"surveillance\" system which appears crucial in controlling tumour growth, since it determines the establishment of small numbers of tumour cells while they can still be easily destroyed.", "contents": "Effect of carrageenan on the non-specific resistance of mice to injected syngeneic tumour cells, alone or in mixtures. The mechanisms of non-specific resistance to syngeneic methylcholanthrene-induced fibrosarcomas of mice were investigated. Results showed that a small tumour graft of 0.05 X 10(5) cells is greatly enhanced in growth when admixed with large numbers of cell fragments, killed cells or viable non-replicating cells. The enhancement of small tumour grafts in cell mixtures was found to be non-specific. Carrageenan, a known anti-macrophage agent, significantly increased tumour growth in normal mice. However, it did not enhance the increased tumour growth of 0.05 X 10(5) cells mixed with 10(6) viable, non-replicating mitomycin C-treated tumour cells. The latter observation indicates that carrageenan and admixed cells interfere with the same tumour-inhibitory mechanism and therefore cannot produce additive effects. The results give support to the concept of a non-specific macrophage \"surveillance\" system which appears crucial in controlling tumour growth, since it determines the establishment of small numbers of tumour cells while they can still be easily destroyed."}
{"id": "PMID:465297", "title": "Total urinary and free serum hydroxyproline in metastatic bone disease.", "content": "The present study examines the possibility of correlation between free serum and urinary total hydroxyproline and whether this correlation can be applied to clinical conditions. The correlation between the two indices was 0.80 (P less than 0.001) in 18 patients, mostly suffering from malignant disease. On comparing the same measurements in 37 patients, all with known metastatic bone disease, we found 29/37 normal results for free serum hydroxyproline, whereas only 2/37 values of urinary total hydroxyproline were normal. The authors therefore conclude that urinary total hydroxyproline, measured as the ratio hydroxyproline/creatinine in a fresh specimen of early-morning urine, is the best index of collagen breakdown in metastatic bone disease and preferable to measurement of free serum hydroxyproline.", "contents": "Total urinary and free serum hydroxyproline in metastatic bone disease. The present study examines the possibility of correlation between free serum and urinary total hydroxyproline and whether this correlation can be applied to clinical conditions. The correlation between the two indices was 0.80 (P less than 0.001) in 18 patients, mostly suffering from malignant disease. On comparing the same measurements in 37 patients, all with known metastatic bone disease, we found 29/37 normal results for free serum hydroxyproline, whereas only 2/37 values of urinary total hydroxyproline were normal. The authors therefore conclude that urinary total hydroxyproline, measured as the ratio hydroxyproline/creatinine in a fresh specimen of early-morning urine, is the best index of collagen breakdown in metastatic bone disease and preferable to measurement of free serum hydroxyproline."}
{"id": "PMID:465298", "title": "Descriptive epidemiology of testicular and prostatic cancer in Los Angeles.", "content": "Data from the Los Angeles County Cancer Surveillance Program (CSP) from 1972 to 1975 were used to study the descriptive epidemiology of testicular cancer and prostatic cancer. The very high black/white ratio and late age peak of cancer of the prostate contrasted sharply with the very low ratio and early age peak of testicular cancer. However, both sites had higher rates among upper occupational and social class groupings. Avalable descriptive and analytical research suggests that the etiology of prostatic cancer is most probably related to hormonal influences rather than to a horizontally transmitted agent, while the etiology of testicular cancer is most probably related to endogenous or exogenous hormonal influences in utero or in infancy, or to in utero exposure to other exogenous agents.", "contents": "Descriptive epidemiology of testicular and prostatic cancer in Los Angeles. Data from the Los Angeles County Cancer Surveillance Program (CSP) from 1972 to 1975 were used to study the descriptive epidemiology of testicular cancer and prostatic cancer. The very high black/white ratio and late age peak of cancer of the prostate contrasted sharply with the very low ratio and early age peak of testicular cancer. However, both sites had higher rates among upper occupational and social class groupings. Avalable descriptive and analytical research suggests that the etiology of prostatic cancer is most probably related to hormonal influences rather than to a horizontally transmitted agent, while the etiology of testicular cancer is most probably related to endogenous or exogenous hormonal influences in utero or in infancy, or to in utero exposure to other exogenous agents."}
{"id": "PMID:465299", "title": "Oesophageal cancer studies in the Caspian Littoral of Iran: results of a case-control study.", "content": "The results are presented of a case-control study conducted in the north of Iran. The main aim was to study factors identified in a previous study as potentially causally related to cancer of the oesophagus. Other tumours (lung, stomach, breast, large bowel, larynx and pharynx) were included to distinguish findings specific for oesophageal cancer from general characteristics of cancer patients, due for example to ascertainment bias, and to verify that expected associations, such as between lung cancer and cigarette smoking, would emerge under the prevailing field conditions. Two controls were chosen per case, matched for village of residence, age, sex and language group, Reinterviewing was performed to a limited extent to assess the accuracy of replies to questionnaires. The following were found not to be associated with oesophageal cancer: consumption of sheep's milk and yoghurt, sesame oil, chewing of nass, making of carpets, use of pregnancy diets, salting and sun-drying of meat and use of wild spinach. The use of opium, bread and tea could not be assessed in the retrospective framework. Strongly associated with risk of oesophageal cancer were low socio-economic status and low intake of fresh fruit and vegetables. The two factors each had an independent effect, and were more marked for oesophageal cancer than for the other tumours.", "contents": "Oesophageal cancer studies in the Caspian Littoral of Iran: results of a case-control study. The results are presented of a case-control study conducted in the north of Iran. The main aim was to study factors identified in a previous study as potentially causally related to cancer of the oesophagus. Other tumours (lung, stomach, breast, large bowel, larynx and pharynx) were included to distinguish findings specific for oesophageal cancer from general characteristics of cancer patients, due for example to ascertainment bias, and to verify that expected associations, such as between lung cancer and cigarette smoking, would emerge under the prevailing field conditions. Two controls were chosen per case, matched for village of residence, age, sex and language group, Reinterviewing was performed to a limited extent to assess the accuracy of replies to questionnaires. The following were found not to be associated with oesophageal cancer: consumption of sheep's milk and yoghurt, sesame oil, chewing of nass, making of carpets, use of pregnancy diets, salting and sun-drying of meat and use of wild spinach. The use of opium, bread and tea could not be assessed in the retrospective framework. Strongly associated with risk of oesophageal cancer were low socio-economic status and low intake of fresh fruit and vegetables. The two factors each had an independent effect, and were more marked for oesophageal cancer than for the other tumours."}
{"id": "PMID:465300", "title": "Pharmacokinetic considerations in testing hypoxic cell radiosensitizers in mouse tumours.", "content": "Bilateral kidney ligation of mice immediately before injection of misonidazole (MIS) prolongs the plasma half-life of this radiosensitizer from about 2 h (in normal mice) to 10-11 h, similar to that in man. Kidney ligation does not, however, change the relative proportions of MIS and its O-demethylated metabolite, Ro-05-9963, for the first 12 h after MIS injection. Kidney ligation was used with the two radiosensitizers, MIS and Ro-05-9963, to investigate the influence of plasma half-life both on peak plasma levels and on the tumour/plasma ratio of sensitizer concentration in the EMT6 mouse tumour. Although the acute LD50 of Ro-05-9963 in normal mice was twice that of MIS, this apparent advantage was offset by peak tumour levels 50% or less of those achieved by equimolar injected doses of MIS. However, by comparing the plasma and tumour levels in mice in which the drug half-lives were prolonged by bilateral kidney ligation, it was concluded that the lower plasma and tumour levels of Ro-05-9963 were a result of its shorter plasma half-life, rather than of an intrinsic barrier to tumour penetration. Because of this rapid clearance, the radiosensitization produced by Ro-05-9963 was less than that produced by equimolar injected doses of MIS. As this difference did not occur in kidney-ligated mice, and hence would not be expected to occur in man, the comparison of MIS and Ro-05-9963 in mice produces an artificially low radiosensitization for Ro-05-9963 and possibly also for other compounds with short plasma half-lives. Although the short plasma half-life of Ro-05-9963 appeared to be responsible for its low peak plasma concentration, it did not produce a low tumour/plasma ratio. Within the limits of plasma nitroimidazole half-lives investigated (0.5-10 h) the tumour/plasma ratio was insensitive to plasma half-life, being 50-70% for both MIS and Ro-05-9963 in both normal and kidney-ligated mice. It is concluded that the common assumption that tumour/plasma ratios of MIS in the mouse are less than those in man is unjustified.", "contents": "Pharmacokinetic considerations in testing hypoxic cell radiosensitizers in mouse tumours. Bilateral kidney ligation of mice immediately before injection of misonidazole (MIS) prolongs the plasma half-life of this radiosensitizer from about 2 h (in normal mice) to 10-11 h, similar to that in man. Kidney ligation does not, however, change the relative proportions of MIS and its O-demethylated metabolite, Ro-05-9963, for the first 12 h after MIS injection. Kidney ligation was used with the two radiosensitizers, MIS and Ro-05-9963, to investigate the influence of plasma half-life both on peak plasma levels and on the tumour/plasma ratio of sensitizer concentration in the EMT6 mouse tumour. Although the acute LD50 of Ro-05-9963 in normal mice was twice that of MIS, this apparent advantage was offset by peak tumour levels 50% or less of those achieved by equimolar injected doses of MIS. However, by comparing the plasma and tumour levels in mice in which the drug half-lives were prolonged by bilateral kidney ligation, it was concluded that the lower plasma and tumour levels of Ro-05-9963 were a result of its shorter plasma half-life, rather than of an intrinsic barrier to tumour penetration. Because of this rapid clearance, the radiosensitization produced by Ro-05-9963 was less than that produced by equimolar injected doses of MIS. As this difference did not occur in kidney-ligated mice, and hence would not be expected to occur in man, the comparison of MIS and Ro-05-9963 in mice produces an artificially low radiosensitization for Ro-05-9963 and possibly also for other compounds with short plasma half-lives. Although the short plasma half-life of Ro-05-9963 appeared to be responsible for its low peak plasma concentration, it did not produce a low tumour/plasma ratio. Within the limits of plasma nitroimidazole half-lives investigated (0.5-10 h) the tumour/plasma ratio was insensitive to plasma half-life, being 50-70% for both MIS and Ro-05-9963 in both normal and kidney-ligated mice. It is concluded that the common assumption that tumour/plasma ratios of MIS in the mouse are less than those in man is unjustified."}
{"id": "PMID:465301", "title": "Ascorbate anion potentiates cytotoxicity of nitro-aromatic compounds under hypoxic and anoxic conditions.", "content": "The nitro-aromatic radiosensitizing drugs are selectively toxic to hypoxic mammalian cells, and this toxicity can be greatly increased by the addition of ascorbate. The ascorbate itself is not toxic to either hypoxic or aerobic cells (as long as catalase is present to prevent the formation of significant concentrations of hydrogen peroxide) and the mixture of ascorbate plus radiosensitizer is not more toxic to aerobic cells. Sulphydryl reducing agents and dithionite have an effect opposite to ascorbate and decrease the toxicity of nitro-aromatic drugs under hypoxic conditions. Sulphydryl reducing agents are also reported to nullify the radiosensitizing properties of nitro-aromatic drugs, in contrast to ascorbate which has no effect on the radiosensitizing properties. The toxicity of nitro-aromatic drugs decreases rapidly with increasing O2 concentration. This decrease is much less rapid when ascorbate is present. The role of ascorbate in this case may be primarily as an O2 scavenger, although it is also possible that the toxic species produced by radiosensitizer-ascorbate mixtures is less easily removed or detoxified by O2.", "contents": "Ascorbate anion potentiates cytotoxicity of nitro-aromatic compounds under hypoxic and anoxic conditions. The nitro-aromatic radiosensitizing drugs are selectively toxic to hypoxic mammalian cells, and this toxicity can be greatly increased by the addition of ascorbate. The ascorbate itself is not toxic to either hypoxic or aerobic cells (as long as catalase is present to prevent the formation of significant concentrations of hydrogen peroxide) and the mixture of ascorbate plus radiosensitizer is not more toxic to aerobic cells. Sulphydryl reducing agents and dithionite have an effect opposite to ascorbate and decrease the toxicity of nitro-aromatic drugs under hypoxic conditions. Sulphydryl reducing agents are also reported to nullify the radiosensitizing properties of nitro-aromatic drugs, in contrast to ascorbate which has no effect on the radiosensitizing properties. The toxicity of nitro-aromatic drugs decreases rapidly with increasing O2 concentration. This decrease is much less rapid when ascorbate is present. The role of ascorbate in this case may be primarily as an O2 scavenger, although it is also possible that the toxic species produced by radiosensitizer-ascorbate mixtures is less easily removed or detoxified by O2."}
{"id": "PMID:465303", "title": "Rupture strength of skin wounds in jaundiced rats.", "content": "The rupture strength of 7-day-old wounds in normal rats, in rats in which there had been a previous skin incision, and in sham bile-duct-ligated and bile-duct-ligated jaundiced groups of rats was studied. It was found that wounds in jaundiced rats were significantly weaker than the wounds in the normal group of rats. Skin wounds in normal animals were significantly stronger than wounds in animals with sham bile-duct ligation. No difference was seen between sham bile-duct-ligated and bile-duct-ligated jaundiced rats.", "contents": "Rupture strength of skin wounds in jaundiced rats. The rupture strength of 7-day-old wounds in normal rats, in rats in which there had been a previous skin incision, and in sham bile-duct-ligated and bile-duct-ligated jaundiced groups of rats was studied. It was found that wounds in jaundiced rats were significantly weaker than the wounds in the normal group of rats. Skin wounds in normal animals were significantly stronger than wounds in animals with sham bile-duct ligation. No difference was seen between sham bile-duct-ligated and bile-duct-ligated jaundiced rats."}
{"id": "PMID:465304", "title": "The effect of antibiotics on the intracellular survival of Staphylococcus aureus in vitro.", "content": "Human blood phagocytes and opsonized Staphylococcus aureus were incubated together in vitro and then treated with the bacteriolytic enzyme lysostaphin in order to remove extracellular bacteria. After washing, the phagocytic cells were incubated for a further 90 min with or without added antibiotics and the effect of these drugs on the survival of sensitive intracellular Staph. aureus measured. Penicillin and cefazolin had no effect on intracellular staphylococcal survival, but rifampicin, streptomycin and gentamicin reduced intracellular bacterial counts. The intracellular survival of antibiotic-resistant strains of Staph. aureus was not affected by the relevant extracellular antibiotics. Pre-incubation of phagocytic cells with either aminoglycosides or rifampicin had no effect on the intracellular survival of antibiotic-sensitive Staph. aureus.", "contents": "The effect of antibiotics on the intracellular survival of Staphylococcus aureus in vitro. Human blood phagocytes and opsonized Staphylococcus aureus were incubated together in vitro and then treated with the bacteriolytic enzyme lysostaphin in order to remove extracellular bacteria. After washing, the phagocytic cells were incubated for a further 90 min with or without added antibiotics and the effect of these drugs on the survival of sensitive intracellular Staph. aureus measured. Penicillin and cefazolin had no effect on intracellular staphylococcal survival, but rifampicin, streptomycin and gentamicin reduced intracellular bacterial counts. The intracellular survival of antibiotic-resistant strains of Staph. aureus was not affected by the relevant extracellular antibiotics. Pre-incubation of phagocytic cells with either aminoglycosides or rifampicin had no effect on the intracellular survival of antibiotic-sensitive Staph. aureus."}
{"id": "PMID:465305", "title": "Uptake of Evans blue-bound albumin in the aorta of oestrogen-treated rats.", "content": "The permeability of the aortic endothelium was studied in oophorectomized female rats following i.m. administration of ethinyloestradiol daily for 10 days. For this purpose a solution of Evans blue: albumin (1%:5%) in Ringer's solution was perfused under controlled pressure through the left ventricle. Areas of blue staining, seen through the intimal surface, were taken to indicate areas of endothelial permeability to albumin. They were traced within the outline of the intimal surface of the whole vessel. The total stained area, expressed as a percentage of the total area of the intimal surface, was determined separately for the various segments in both the thoracic and abdominal aortas. Statistical analysis of data indicated that the uptake of Evans blue-bound albumin in the aortic wall was significantly increased in oestrogen-treated rats as compared to their control. This phenomenon was observed throughout the aorta except for its middle abdominal segment. Also its magnitude was comparable in all segments. These results strongly suggest that endothelial permeability to albumin was increased following treatment with synthetic oestrogen. This would enhance the influex of growth-promoting factors and thus play a role in the pathogenesis of proliferative lesions in the arterial wall associated with oestrogen administration in humans as well as in experimental animals.", "contents": "Uptake of Evans blue-bound albumin in the aorta of oestrogen-treated rats. The permeability of the aortic endothelium was studied in oophorectomized female rats following i.m. administration of ethinyloestradiol daily for 10 days. For this purpose a solution of Evans blue: albumin (1%:5%) in Ringer's solution was perfused under controlled pressure through the left ventricle. Areas of blue staining, seen through the intimal surface, were taken to indicate areas of endothelial permeability to albumin. They were traced within the outline of the intimal surface of the whole vessel. The total stained area, expressed as a percentage of the total area of the intimal surface, was determined separately for the various segments in both the thoracic and abdominal aortas. Statistical analysis of data indicated that the uptake of Evans blue-bound albumin in the aortic wall was significantly increased in oestrogen-treated rats as compared to their control. This phenomenon was observed throughout the aorta except for its middle abdominal segment. Also its magnitude was comparable in all segments. These results strongly suggest that endothelial permeability to albumin was increased following treatment with synthetic oestrogen. This would enhance the influex of growth-promoting factors and thus play a role in the pathogenesis of proliferative lesions in the arterial wall associated with oestrogen administration in humans as well as in experimental animals."}
{"id": "PMID:465306", "title": "An autoradiographic study of 3H-thymidine incorporation and distribution in the dermis of healing skin incisions in the pig.", "content": "The dermal response to injury in healing incisional wounds aged from 1 to 24 days was studied by in vitro and in vivo 3H-thymidine (3HT) labelling and the use of colcemid to induce metaphase arrest. In vitro 3HT labelling provided mean numbers (+/- s.e. mean) of labelled dermal cells/section within the incision and in 3 zones in the dermis up to 0.875 mm lateral to the incision. There was a progressive increase in the numbers of DNA-synthesizing cells in the incisions as granulation tissue developed from Day 1 to Day 7, and a sharp decline by Day 9. Immediately adjacent to the incision there was a dramatic increase in the number of labelled cells on Day 2, with reduced numbers laterally. This response, which was largely maintained up to 7 days, paralleled the cellular reaction around blood vessels and skin appendages but also included labelled dermal fibroblasts. The in vivo 3HT study, taken in conjunction with the colcemid and in vitro 3HT studies, supported the idea that during the development of granulation tissue there is a continual recruitment of paraincisional cells, including dermal fibroblasts, either by direct migration or by division and then migration, as well as by continued mitosis of cells already within the incision.", "contents": "An autoradiographic study of 3H-thymidine incorporation and distribution in the dermis of healing skin incisions in the pig. The dermal response to injury in healing incisional wounds aged from 1 to 24 days was studied by in vitro and in vivo 3H-thymidine (3HT) labelling and the use of colcemid to induce metaphase arrest. In vitro 3HT labelling provided mean numbers (+/- s.e. mean) of labelled dermal cells/section within the incision and in 3 zones in the dermis up to 0.875 mm lateral to the incision. There was a progressive increase in the numbers of DNA-synthesizing cells in the incisions as granulation tissue developed from Day 1 to Day 7, and a sharp decline by Day 9. Immediately adjacent to the incision there was a dramatic increase in the number of labelled cells on Day 2, with reduced numbers laterally. This response, which was largely maintained up to 7 days, paralleled the cellular reaction around blood vessels and skin appendages but also included labelled dermal fibroblasts. The in vivo 3HT study, taken in conjunction with the colcemid and in vitro 3HT studies, supported the idea that during the development of granulation tissue there is a continual recruitment of paraincisional cells, including dermal fibroblasts, either by direct migration or by division and then migration, as well as by continued mitosis of cells already within the incision."}
{"id": "PMID:465307", "title": "Cyproterone acetate-promoted prevention of renal cortical necrosis following testosterone and vasopressin administration.", "content": "Bilateral focal renal cortical necrosis was observed after vasopressin administration in rats pretreated for 10 days with testosterone phenyl propionate. When the androgen-receptor blocking-agent cyproterone acetate was administered together with the testosterone, the subsequent vasopressin treatment did not cause renal cortical necrosis. The results suggest the role of the androgen receptors in the kidney in the induction of the phenomenon.", "contents": "Cyproterone acetate-promoted prevention of renal cortical necrosis following testosterone and vasopressin administration. Bilateral focal renal cortical necrosis was observed after vasopressin administration in rats pretreated for 10 days with testosterone phenyl propionate. When the androgen-receptor blocking-agent cyproterone acetate was administered together with the testosterone, the subsequent vasopressin treatment did not cause renal cortical necrosis. The results suggest the role of the androgen receptors in the kidney in the induction of the phenomenon."}
{"id": "PMID:465308", "title": "Effect of different factors modifying the activity of some enzyme systems of the endoplasmic reticulum on the sensitivity of cell organelles against the damaging action of chemical agents. II. Studies with chlorpromazine, 2,4-dinitrophenol, phenobarbital and DDT.", "content": "Further investigations on the effect of different stress factors on the stability of intracellular membranes were carried out. Large granule fractions derived from livers of sleep-deprived and dehydrated rats and subjected to preincubation at 37 degrees and pH 5 were shown to release latent acid phosphatase with a delayed rate indicating an increased lysosomal stability towards acid media conditioning. Lysosomes of such animals, however, were found to be more sensitive to mechanical treatments (homogenization procedure in this case) than that of controls, a conclusion made on the basis of enhanced \"free\" and nonsedimentable phosphatase activities in liver homogenates. The stress factors which previously were included in the group of modifiers of the activity of the endoplasmic reticulum-located enzymes caused some changes in the action of certain chemicals on membranes. Earlier such changes were elicited for carbon tetrachloride and only on low-temperature-conditioned rats for chlorpromazine. The present results show that stress factors studied result in deviations (different in extent and in direction) from the usual effects of chlorpromazine, 2,4-dinitrophenol, phenobarbital and DDT on liver lysosomes and peroxisomes.", "contents": "Effect of different factors modifying the activity of some enzyme systems of the endoplasmic reticulum on the sensitivity of cell organelles against the damaging action of chemical agents. II. Studies with chlorpromazine, 2,4-dinitrophenol, phenobarbital and DDT. Further investigations on the effect of different stress factors on the stability of intracellular membranes were carried out. Large granule fractions derived from livers of sleep-deprived and dehydrated rats and subjected to preincubation at 37 degrees and pH 5 were shown to release latent acid phosphatase with a delayed rate indicating an increased lysosomal stability towards acid media conditioning. Lysosomes of such animals, however, were found to be more sensitive to mechanical treatments (homogenization procedure in this case) than that of controls, a conclusion made on the basis of enhanced \"free\" and nonsedimentable phosphatase activities in liver homogenates. The stress factors which previously were included in the group of modifiers of the activity of the endoplasmic reticulum-located enzymes caused some changes in the action of certain chemicals on membranes. Earlier such changes were elicited for carbon tetrachloride and only on low-temperature-conditioned rats for chlorpromazine. The present results show that stress factors studied result in deviations (different in extent and in direction) from the usual effects of chlorpromazine, 2,4-dinitrophenol, phenobarbital and DDT on liver lysosomes and peroxisomes."}
{"id": "PMID:465309", "title": "Calcium-related changes in the ultrastructure of mammalian myocardium.", "content": "Isolated rat hearts, perfused with Ca2+-free medium for 5 min, were perfusion-fixed for ultrastructural studies. Increased pinocytotoic activity and characteristic changes in the staining of the intercalated disc coupled with conspicuous staining of the middle lamina in the tight junctions in these hearts indicated an alteration in the sarcolemmal activity as well as cell--cell relationship. The intracellular effects of Ca2+-free perfusion were indicated by the presence of an active Golgi body as well as the loss of heterogenic staining of the nucleoplasm. The latter appears to be a direct consequence of Ca2+ depletion of the cell, as heterogeneity reappeared upon reintroduction of the calcium in the perfusion medium. The extent of structural damage upon reintroduction of calcium was dependent upon the extracellular calcium concentration. These observations suggest that perfusion with Ca2+-free medium causes some membrane changes in the heart which probably make it more vulnerable to the reintroduction of calcium. Furthermore, the structural damage observed upon reintroduction of calcium appeared to be related to the amount of calcium overload.", "contents": "Calcium-related changes in the ultrastructure of mammalian myocardium. Isolated rat hearts, perfused with Ca2+-free medium for 5 min, were perfusion-fixed for ultrastructural studies. Increased pinocytotoic activity and characteristic changes in the staining of the intercalated disc coupled with conspicuous staining of the middle lamina in the tight junctions in these hearts indicated an alteration in the sarcolemmal activity as well as cell--cell relationship. The intracellular effects of Ca2+-free perfusion were indicated by the presence of an active Golgi body as well as the loss of heterogenic staining of the nucleoplasm. The latter appears to be a direct consequence of Ca2+ depletion of the cell, as heterogeneity reappeared upon reintroduction of the calcium in the perfusion medium. The extent of structural damage upon reintroduction of calcium was dependent upon the extracellular calcium concentration. These observations suggest that perfusion with Ca2+-free medium causes some membrane changes in the heart which probably make it more vulnerable to the reintroduction of calcium. Furthermore, the structural damage observed upon reintroduction of calcium appeared to be related to the amount of calcium overload."}
{"id": "PMID:465310", "title": "Mast cell population density, blood vessel density and histamine content in normal human skin.", "content": "Mast cell population density was determined in normal skin from two regions of the arm of several healthy men and compared with blood vessel density and histamine concentration in the same sites. Mast cell and blood vessel counts were made in 1--1.5 micrometer thick plastic sections, by light microscopy and tissue-histamine concentrations were determined by automated fluorimetric analysis. Statistically significant correlations were found between mast cell counts, blood vessel counts and histamine content in skin from the upper arm but no similar correlations were obtained in the forearm. Anatomical differences between the two sites may have been the cause of this discrepancy. Wide variations in mast cell counts and blood vessel density were found in different sections from the same biopsy samples which confirms the notion that dermal mast cells are unevenly distributed. Analysis of variance of the mast cell counts showed that the variance between sections from different blocks from the same biopsy samples was greater than the variance between adjacent biopsies. There was also a marked variation in the histamine content between biopsy samples from sites only 2 cm apart in the same subject.", "contents": "Mast cell population density, blood vessel density and histamine content in normal human skin. Mast cell population density was determined in normal skin from two regions of the arm of several healthy men and compared with blood vessel density and histamine concentration in the same sites. Mast cell and blood vessel counts were made in 1--1.5 micrometer thick plastic sections, by light microscopy and tissue-histamine concentrations were determined by automated fluorimetric analysis. Statistically significant correlations were found between mast cell counts, blood vessel counts and histamine content in skin from the upper arm but no similar correlations were obtained in the forearm. Anatomical differences between the two sites may have been the cause of this discrepancy. Wide variations in mast cell counts and blood vessel density were found in different sections from the same biopsy samples which confirms the notion that dermal mast cells are unevenly distributed. Analysis of variance of the mast cell counts showed that the variance between sections from different blocks from the same biopsy samples was greater than the variance between adjacent biopsies. There was also a marked variation in the histamine content between biopsy samples from sites only 2 cm apart in the same subject."}
{"id": "PMID:465311", "title": "Distribution of mast cells in human dermis: development of a mapping technique.", "content": "A new method was developed to define the distribution of mast cells in normal human skin. The population density was determined in a number of zones of different depth in the dermis. Two regions of the same limb were studied systematically. Both were shown to have mast cell populations of similar size and distribution, with a maximum density immediately below the dermo-epidermal junction, gradually falling to a minimum density in the deeper layers of the dermis. Small secondary peaks were less clearly defined at the base of the dermis. These findings should provide baseline data for comparisons with mast cell population size and distribution in different areas of normal skin, or in similar areas in clinical disorders or experimental conditions.", "contents": "Distribution of mast cells in human dermis: development of a mapping technique. A new method was developed to define the distribution of mast cells in normal human skin. The population density was determined in a number of zones of different depth in the dermis. Two regions of the same limb were studied systematically. Both were shown to have mast cell populations of similar size and distribution, with a maximum density immediately below the dermo-epidermal junction, gradually falling to a minimum density in the deeper layers of the dermis. Small secondary peaks were less clearly defined at the base of the dermis. These findings should provide baseline data for comparisons with mast cell population size and distribution in different areas of normal skin, or in similar areas in clinical disorders or experimental conditions."}
{"id": "PMID:465312", "title": "The aetiology of juvenile plantar dermatosis.", "content": "Studies of the sweating mechanism in juvenile plantar dermatosis have demonstrated features of miliaria, including inhibition of sweating and occlusion of the sweat coil in the horny layer of the epidermis. These are considered to be a sequel to changes in the composition of children's shoes and hose. Possible remedies are suggested.", "contents": "The aetiology of juvenile plantar dermatosis. Studies of the sweating mechanism in juvenile plantar dermatosis have demonstrated features of miliaria, including inhibition of sweating and occlusion of the sweat coil in the horny layer of the epidermis. These are considered to be a sequel to changes in the composition of children's shoes and hose. Possible remedies are suggested."}
{"id": "PMID:465313", "title": "Surgical conversion of herpes simplex from an epidermal to a dermal disease.", "content": "A new post-surgical form of recurrent herpes simplex is described. It appeared as an erythematous bulla at sites of recurrent herpes from which the prior epidermal lesion had been transversely excised. Histologically, it was characterized by a lesion-free epidermis, a large subepidermal bulla, and a marked inflammatory response in the uppermost dermis, complete with diagnostic multinucleate giant cells. It is postulated that epidermal surgery leaves the area immune to future direct herpetic involvement of the epidermis because it denervates the epidermis. The possibility is raised that such dermal forms of herpes may also occur in the natural course of the disease.", "contents": "Surgical conversion of herpes simplex from an epidermal to a dermal disease. A new post-surgical form of recurrent herpes simplex is described. It appeared as an erythematous bulla at sites of recurrent herpes from which the prior epidermal lesion had been transversely excised. Histologically, it was characterized by a lesion-free epidermis, a large subepidermal bulla, and a marked inflammatory response in the uppermost dermis, complete with diagnostic multinucleate giant cells. It is postulated that epidermal surgery leaves the area immune to future direct herpetic involvement of the epidermis because it denervates the epidermis. The possibility is raised that such dermal forms of herpes may also occur in the natural course of the disease."}
{"id": "PMID:465314", "title": "The dermatopathology of Cowden's syndrome.", "content": "During the past 2 years we have studied eighty-nine biopsy specimens from nineteen patients with Cowden's syndrome. Among fifty-three facial lesions, twenty-nine were trichilemmomas. Twenty-three of the others were consistent with trichilemmoma, showed a non-specific benign verrucous acanthoma, or were not diagnostic; one was a blue naevus. All fourteen oral mucosal biopsy specimens were benign fibromas. Nineteen of twenty-two biopsy specimens from the hands and feet showed the pattern of benign keratosis. Multiple trichilemmomas were found in all patients with Cowden's  syndrome, but at times several biopsy specimens were required before a diagnostic picture was uncovered. All patients with multiple facial trichilemmomas were found to have Cowden's syndrome. The combination of multiple facial trichilemmomas, oral fibromas, and benign acral keratoses enables one to diagnose Cowden's syndrome at a stage before serious internal complications develop.", "contents": "The dermatopathology of Cowden's syndrome. During the past 2 years we have studied eighty-nine biopsy specimens from nineteen patients with Cowden's syndrome. Among fifty-three facial lesions, twenty-nine were trichilemmomas. Twenty-three of the others were consistent with trichilemmoma, showed a non-specific benign verrucous acanthoma, or were not diagnostic; one was a blue naevus. All fourteen oral mucosal biopsy specimens were benign fibromas. Nineteen of twenty-two biopsy specimens from the hands and feet showed the pattern of benign keratosis. Multiple trichilemmomas were found in all patients with Cowden's  syndrome, but at times several biopsy specimens were required before a diagnostic picture was uncovered. All patients with multiple facial trichilemmomas were found to have Cowden's syndrome. The combination of multiple facial trichilemmomas, oral fibromas, and benign acral keratoses enables one to diagnose Cowden's syndrome at a stage before serious internal complications develop."}
{"id": "PMID:465315", "title": "Multiple apocrine cystadenomas.", "content": "A 56-year-old man had approximately forty apocrine cystadenomas of the face and ears. Clinical examination and light microscopic findings were typical for apocrine cystadenomas. Electron microscopic findings showed an absence of annulate lamellae, thus differing from the only previously reported study. Review of the literature indicates that a large number of apocrine cystadenomas, such as on our patient, has not been reported previously.", "contents": "Multiple apocrine cystadenomas. A 56-year-old man had approximately forty apocrine cystadenomas of the face and ears. Clinical examination and light microscopic findings were typical for apocrine cystadenomas. Electron microscopic findings showed an absence of annulate lamellae, thus differing from the only previously reported study. Review of the literature indicates that a large number of apocrine cystadenomas, such as on our patient, has not been reported previously."}
{"id": "PMID:465316", "title": "HLA system in relation to carbohydrate metabolism in lichen planus.", "content": "HLA antigens were determined in a group of fifty-two patients with lichen planus. In the group as a whole the frequency of A28 was increased but not significantly so. When the patients were subdivided according to the results of an oral glucose tolerance test, however, a highly significant increase in the frequency of A28 was found among the non-diabetic patients.", "contents": "HLA system in relation to carbohydrate metabolism in lichen planus. HLA antigens were determined in a group of fifty-two patients with lichen planus. In the group as a whole the frequency of A28 was increased but not significantly so. When the patients were subdivided according to the results of an oral glucose tolerance test, however, a highly significant increase in the frequency of A28 was found among the non-diabetic patients."}
{"id": "PMID:465317", "title": "Percutaneous absorption in experimental epidermal disease.", "content": "Little is known about the absorption of topical drugs in epidermal disease associated with epidermal proliferation and altered keratinization. An abnormal hairless mouse epidermis was produced by three different methods: ultraviolet light irradiation, topical vitamin A acid and topical acetic acid. An increased epidermal thickness resulted and the in vitro percutaneous absorption of 0.1% (4-14C) hydrocortisone was found to be increased when compared with normal hairless mouse epidermis. This is evidence for defective skin barrier function in experimental epidermal disease.", "contents": "Percutaneous absorption in experimental epidermal disease. Little is known about the absorption of topical drugs in epidermal disease associated with epidermal proliferation and altered keratinization. An abnormal hairless mouse epidermis was produced by three different methods: ultraviolet light irradiation, topical vitamin A acid and topical acetic acid. An increased epidermal thickness resulted and the in vitro percutaneous absorption of 0.1% (4-14C) hydrocortisone was found to be increased when compared with normal hairless mouse epidermis. This is evidence for defective skin barrier function in experimental epidermal disease."}
{"id": "PMID:465318", "title": "Pemphigus-like antibodies: a report of two cases.", "content": "Two patients with high titres of circulating intercellular antibodies are described. One had bullous mycosis fungoides and the other had bullous impetigo. In each case, as the underlying disease responded to treatment, the titre of antibodies fell.", "contents": "Pemphigus-like antibodies: a report of two cases. Two patients with high titres of circulating intercellular antibodies are described. One had bullous mycosis fungoides and the other had bullous impetigo. In each case, as the underlying disease responded to treatment, the titre of antibodies fell."}
{"id": "PMID:465319", "title": "Persistent warts protected from immune attack by a blocking factor.", "content": "Severe warts of 20 years duration, highly resistant to therapy, were found to contain a soluble factor which blocked the local expression of cellular immunity, without affecting systemic immune responsiveness. This finding enabled us to propose therapy by infection, and this had a limited success.", "contents": "Persistent warts protected from immune attack by a blocking factor. Severe warts of 20 years duration, highly resistant to therapy, were found to contain a soluble factor which blocked the local expression of cellular immunity, without affecting systemic immune responsiveness. This finding enabled us to propose therapy by infection, and this had a limited success."}
{"id": "PMID:465352", "title": "Membrane abnormalities of pyruvate kinase deficient red cells.", "content": "Two experimental systems have been applied to the study of PK-deficient red cells in an attempt to reveal possible membrane abnormalities of these erythrocytes: (1) the glycoprotein self-digestion of intact erythrocytes during in vitro incubation at 37 degrees C; (2) the red cell sensitivity to the lytic action of mouse macrophages. PK-deficient erythrocytes display a more precocious than normal membrane glycoprotein self-digestion and are much more susceptible than normal to the cytotoxic activity of mouse macrophages. This latter effect is more pronounced in young than in old PK-deficient red cells. These observations indicate the existence of membrane abnormality in PK-deficient red cells other than those so far suspected.", "contents": "Membrane abnormalities of pyruvate kinase deficient red cells. Two experimental systems have been applied to the study of PK-deficient red cells in an attempt to reveal possible membrane abnormalities of these erythrocytes: (1) the glycoprotein self-digestion of intact erythrocytes during in vitro incubation at 37 degrees C; (2) the red cell sensitivity to the lytic action of mouse macrophages. PK-deficient erythrocytes display a more precocious than normal membrane glycoprotein self-digestion and are much more susceptible than normal to the cytotoxic activity of mouse macrophages. This latter effect is more pronounced in young than in old PK-deficient red cells. These observations indicate the existence of membrane abnormality in PK-deficient red cells other than those so far suspected."}
{"id": "PMID:465353", "title": "The heterogeneity of normal Hb A2-beta thalassaemia in Greece.", "content": "Nine patients have been observed with homozygous beta thalassaemia in each of whom one parent has a normal level of Hb A2. On the basis of clinical, haematological and globin chain synthesis studies these families have been divided into two groups. Group 1 (six families). Heterozygotes for normal Hb A2-beta thalassaemia in this group showed minimal red cell abnormalities, normal osmotic fragility but imbalanced globin chain synthesis (alpha/beta=1.6), and appear to correspond to previous descriptions of 'silent' beta thalassaemia. Double heterozygotes with high Hb A2-beta thalassaemia have a clinical picture of mild beta thalassaemia intermedia characterized by relatively low levels of Hb F (less than 20%) and gamma chain synthesis. Group 2 (three families). beta Thalassaemia heterozygotes with normal HbA2 levels in this group showed more marked red cell abnormalities, decreased osmotic fragility and more imbalanced globin chain synthesis (alpha/beta=2.5) than those in group I. Double heterozygotes with high Hb A2-beta thalassaemia are more severely affected and are transfusion dependent. Haemoglobin F and gamma chain synthesis are high in these cases. The frequency of normal Hb A2-beta thalassaemia in Greece may be as high as 10% of all beta thalassaemia genes and this poses a significant problem for genetic counselling. Various molecular mechanisms are discussed which could account for the heterogeneity within these disorders.", "contents": "The heterogeneity of normal Hb A2-beta thalassaemia in Greece. Nine patients have been observed with homozygous beta thalassaemia in each of whom one parent has a normal level of Hb A2. On the basis of clinical, haematological and globin chain synthesis studies these families have been divided into two groups. Group 1 (six families). Heterozygotes for normal Hb A2-beta thalassaemia in this group showed minimal red cell abnormalities, normal osmotic fragility but imbalanced globin chain synthesis (alpha/beta=1.6), and appear to correspond to previous descriptions of 'silent' beta thalassaemia. Double heterozygotes with high Hb A2-beta thalassaemia have a clinical picture of mild beta thalassaemia intermedia characterized by relatively low levels of Hb F (less than 20%) and gamma chain synthesis. Group 2 (three families). beta Thalassaemia heterozygotes with normal HbA2 levels in this group showed more marked red cell abnormalities, decreased osmotic fragility and more imbalanced globin chain synthesis (alpha/beta=2.5) than those in group I. Double heterozygotes with high Hb A2-beta thalassaemia are more severely affected and are transfusion dependent. Haemoglobin F and gamma chain synthesis are high in these cases. The frequency of normal Hb A2-beta thalassaemia in Greece may be as high as 10% of all beta thalassaemia genes and this poses a significant problem for genetic counselling. Various molecular mechanisms are discussed which could account for the heterogeneity within these disorders."}
{"id": "PMID:465355", "title": "Recommendations for fetal haemoglobin reference preparations and fetal haemogloblin determination by the alkali denaturation method.", "content": "Lyophilized haemoglobin mixtures containing HbF and HbA were investigated as reference preparations in an interlaboratory collaborative study. Haemiglobincyanide mixtures in Drabkin's solution with 450-550 mg Hb/dl and 0.2-60% HbF were divided into samples of 10 ml, lyophilized in plastic containers and stored without vacuum at -20 degrees C. After dissolution in 10 ml distilled water no change in spectral and electrophoretic properties or turbidity was observed; stable values for the total Hb concentration and HbF content were obtained over a period of over 2 years. These HbF standards can be prepared in reference laboratories and are recommended for quality control in HbF determination by alkali denaturation. For fetal haemoglobin determination the alkali denaturation technique of Berke, Marti and Schlicht is recommended for quantitation of HbF concentrations, at least between 2 and 40%. For values below 2% radioimmunoassay and for concentrations above 40% chromatographic procedures are more accurate. However, these methods will not be applicable in smaller haematological laboratories and in developing countries.", "contents": "Recommendations for fetal haemoglobin reference preparations and fetal haemogloblin determination by the alkali denaturation method. Lyophilized haemoglobin mixtures containing HbF and HbA were investigated as reference preparations in an interlaboratory collaborative study. Haemiglobincyanide mixtures in Drabkin's solution with 450-550 mg Hb/dl and 0.2-60% HbF were divided into samples of 10 ml, lyophilized in plastic containers and stored without vacuum at -20 degrees C. After dissolution in 10 ml distilled water no change in spectral and electrophoretic properties or turbidity was observed; stable values for the total Hb concentration and HbF content were obtained over a period of over 2 years. These HbF standards can be prepared in reference laboratories and are recommended for quality control in HbF determination by alkali denaturation. For fetal haemoglobin determination the alkali denaturation technique of Berke, Marti and Schlicht is recommended for quantitation of HbF concentrations, at least between 2 and 40%. For values below 2% radioimmunoassay and for concentrations above 40% chromatographic procedures are more accurate. However, these methods will not be applicable in smaller haematological laboratories and in developing countries."}
{"id": "PMID:465356", "title": "Thrombin receptors of human platelets: thrombin binding and antithrombin properties of glycoprotein I.", "content": "Washed human platelets were solubilized and the proteins were separated by preparative gel electrophoresis in the presence of sodium dodecyl sulphate. The gel was cut into slices and the effect of the eluted proteins on the clotting of fibrinogen by thrombin was evaluated. The isolate from only one gel slice strongly inhibited the clotting of fibrinogen. The prolongation of the clotting time was dependent on the concentration of the protein and reached a plateau around 5 microgram. Gel electrophoresis of this isolate showed a prominent glycoprotein with an apparent Mr=150 000. Gel filtration studies with [125I]thrombin showed that the protein isolate bound a significant amount of thrombin which could be displaced with unlabelled thrombin. Another preparation from the same gel or purified gamma-globulin did not bind thrombin or prolong the clotting time of fibrinogen. Glycoprotein I was isolated from human platelets by affinity chromatography on lectin-Sepharose columns. The isolated glycoprotein prolonged the clotting of fibrinogen and bound [125I]thrombin which could be displaced by unlabelled thrombin. It is proposed that the high affinity receptor of thrombin on human platelets is glycoprotein I. In addition, the antithrombin activity of intact platelets is due to binding of thrombin to this glycoprotein.", "contents": "Thrombin receptors of human platelets: thrombin binding and antithrombin properties of glycoprotein I. Washed human platelets were solubilized and the proteins were separated by preparative gel electrophoresis in the presence of sodium dodecyl sulphate. The gel was cut into slices and the effect of the eluted proteins on the clotting of fibrinogen by thrombin was evaluated. The isolate from only one gel slice strongly inhibited the clotting of fibrinogen. The prolongation of the clotting time was dependent on the concentration of the protein and reached a plateau around 5 microgram. Gel electrophoresis of this isolate showed a prominent glycoprotein with an apparent Mr=150 000. Gel filtration studies with [125I]thrombin showed that the protein isolate bound a significant amount of thrombin which could be displaced with unlabelled thrombin. Another preparation from the same gel or purified gamma-globulin did not bind thrombin or prolong the clotting time of fibrinogen. Glycoprotein I was isolated from human platelets by affinity chromatography on lectin-Sepharose columns. The isolated glycoprotein prolonged the clotting of fibrinogen and bound [125I]thrombin which could be displaced by unlabelled thrombin. It is proposed that the high affinity receptor of thrombin on human platelets is glycoprotein I. In addition, the antithrombin activity of intact platelets is due to binding of thrombin to this glycoprotein."}
{"id": "PMID:465357", "title": "Blood eosinophils and eosinophil cationic protein after acute myocardial infarction or corticosteroid administration.", "content": "Measurements of eosinophil cationic protein in serum (S-ECP) have been made in patients with acute myocardial infarction. In spite of low numbers of blood eosinophils in the acute phase of the disease S-ECP levels fluctuated widely with often markedly raised levels. Peak levels of S-ECP were significantly correlated (P less than 0.001) to peak numbers of blood eosinophils, although the former usually occurred within the first 2-3 d of illness and the latter usually after the sixth day. Intravenous injection of 0.5 g methylprednisolone in healthy individuals reduced blood eosinophil counts for at least 24 h, but S-ECP levels remained within normal limits. These results suggest that ECP is released from eosinophils in vivo. The raised levels of S-ECP in patients with acute myocardial infarction are probably due to the active participation of eosinophils in the inflammatory process.", "contents": "Blood eosinophils and eosinophil cationic protein after acute myocardial infarction or corticosteroid administration. Measurements of eosinophil cationic protein in serum (S-ECP) have been made in patients with acute myocardial infarction. In spite of low numbers of blood eosinophils in the acute phase of the disease S-ECP levels fluctuated widely with often markedly raised levels. Peak levels of S-ECP were significantly correlated (P less than 0.001) to peak numbers of blood eosinophils, although the former usually occurred within the first 2-3 d of illness and the latter usually after the sixth day. Intravenous injection of 0.5 g methylprednisolone in healthy individuals reduced blood eosinophil counts for at least 24 h, but S-ECP levels remained within normal limits. These results suggest that ECP is released from eosinophils in vivo. The raised levels of S-ECP in patients with acute myocardial infarction are probably due to the active participation of eosinophils in the inflammatory process."}
{"id": "PMID:465359", "title": "Metabolic changes in red blood cells in malignant lymphomas.", "content": "Serum copper levels (SCL) which are concomitantly related to red blood cell free copper are significantly increased in some malignant lymphomas in the phase of activity. This results in a profound inhibition of red cell key glycolytic enzymes, hexokinase (Hx) being the most sensitive. Fifteen patients (eight with Hodgkin's disease and seven with non-Hodgkin's lymphoma) were studied for serum and red cell copper concentrations and Hx activity. The mean red cell life span was determined using 51Cr labelled red cells. The resulting data shows that in active disease an increase in SCL was associated with a decrease in Hx activity and a shortened red cell survival. In these cases there was no evidence of autoimmune phenomena or of direct bone marrow involvement by the disease. It is suggested that the increase in copper levels results in a shortened red cell life span through a copper-induced inhibition of red cell Hx.", "contents": "Metabolic changes in red blood cells in malignant lymphomas. Serum copper levels (SCL) which are concomitantly related to red blood cell free copper are significantly increased in some malignant lymphomas in the phase of activity. This results in a profound inhibition of red cell key glycolytic enzymes, hexokinase (Hx) being the most sensitive. Fifteen patients (eight with Hodgkin's disease and seven with non-Hodgkin's lymphoma) were studied for serum and red cell copper concentrations and Hx activity. The mean red cell life span was determined using 51Cr labelled red cells. The resulting data shows that in active disease an increase in SCL was associated with a decrease in Hx activity and a shortened red cell survival. In these cases there was no evidence of autoimmune phenomena or of direct bone marrow involvement by the disease. It is suggested that the increase in copper levels results in a shortened red cell life span through a copper-induced inhibition of red cell Hx."}
{"id": "PMID:465360", "title": "Bone marrow fibre production in myelofibrosis: a quantitative study.", "content": "A digital image processing technique has been used to quantitate bone marrow fibre in histological material (both biopsy and necropsy) from 17 cases of myelofibrosis. An attempt was made to correlate the results of these measurements with other diagnostically relevant parameters. Bone marrow fibre was found to correlate significantly with absolute neutrophil count, the absolute count of immature granulocytic cells and with the number of immature granulocytic cells expressed as a percentage of the total leucocyte count. There was no significant correlation between marrow fibre content and platelet count for the series as a whole, but for those cases in which measurements were made following splenectomy these parameters were highly correlated. Bone marrow fibre content did not appear to correlate with liver or spleen weight at necropsy, nor with the duration of clinical manifestations of the disease. The significance of these findings is discussed.", "contents": "Bone marrow fibre production in myelofibrosis: a quantitative study. A digital image processing technique has been used to quantitate bone marrow fibre in histological material (both biopsy and necropsy) from 17 cases of myelofibrosis. An attempt was made to correlate the results of these measurements with other diagnostically relevant parameters. Bone marrow fibre was found to correlate significantly with absolute neutrophil count, the absolute count of immature granulocytic cells and with the number of immature granulocytic cells expressed as a percentage of the total leucocyte count. There was no significant correlation between marrow fibre content and platelet count for the series as a whole, but for those cases in which measurements were made following splenectomy these parameters were highly correlated. Bone marrow fibre content did not appear to correlate with liver or spleen weight at necropsy, nor with the duration of clinical manifestations of the disease. The significance of these findings is discussed."}
{"id": "PMID:465361", "title": "Haem synthesis in sideroblastic anaemia.", "content": "Measurement of delta-aminolaevulinic acid (ALA) synthetase has been performed in bone marrow of 24 normal subjects and 20 patients with sideroblastic anaemia (five congenital, two pyridoxine-responsive, 12 primary acquired and one secondary form due to isoniazid therapy). ALA-synthetase activity without added pyridoxal-phosphate in vitro was decreased in four of the congenital cases, and in all 12 primary acquired cases. In two of these 16 cases with low ALA-synthetase activity and in a case with sideroblastic change secondary to isoniazid therapy, the enzyme activity was corrected to normal by pyridoxal-phosphate in vitro. ALA-synthetase activity was normal in both cases of pyridoxine-responsive anaemia while they were receiving pyridoxine therapy but in one of these cases, studied at repeated intervals after withdrawal of pyridoxine therapy, the bone marrow ALA-synthetase activity fell to very low levels unless pyridoxal-phosphate was added in vitro. In this case the Km of the enzyme for pyridoxal-phosphate was substantially greater than that of a control sample. Haem synthetase activity was reduced in the bone marrow of two of six cases of primary acquired sideroblastic anaemia and in one congenital case tested but was normal in the pyridoxine-responsive patient studied on pyridoxine therapy. Nevertheless, this patient showed reduced incorporation of 14C-labelled delta-aminolaevulinic acid into bone marrow haem. It is concluded that both congenital and primary acquired sideroblastic anaemia are a heterogenous group of disorders, with different defects in haem synthesis, reduced activity of delta-aminolaevulinic acid synthetase being common but not invariable in both types. Pyridoxine-responsive (congenital) sideroblastic anaemia may be due to the presence of an abnormal ALA-synthetase apoenzyme which requires excessive amounts of the coenzyme, pyridoxal-phosphate, to achieve normal activity; other abnormalities of haem synthesis may also be present.", "contents": "Haem synthesis in sideroblastic anaemia. Measurement of delta-aminolaevulinic acid (ALA) synthetase has been performed in bone marrow of 24 normal subjects and 20 patients with sideroblastic anaemia (five congenital, two pyridoxine-responsive, 12 primary acquired and one secondary form due to isoniazid therapy). ALA-synthetase activity without added pyridoxal-phosphate in vitro was decreased in four of the congenital cases, and in all 12 primary acquired cases. In two of these 16 cases with low ALA-synthetase activity and in a case with sideroblastic change secondary to isoniazid therapy, the enzyme activity was corrected to normal by pyridoxal-phosphate in vitro. ALA-synthetase activity was normal in both cases of pyridoxine-responsive anaemia while they were receiving pyridoxine therapy but in one of these cases, studied at repeated intervals after withdrawal of pyridoxine therapy, the bone marrow ALA-synthetase activity fell to very low levels unless pyridoxal-phosphate was added in vitro. In this case the Km of the enzyme for pyridoxal-phosphate was substantially greater than that of a control sample. Haem synthetase activity was reduced in the bone marrow of two of six cases of primary acquired sideroblastic anaemia and in one congenital case tested but was normal in the pyridoxine-responsive patient studied on pyridoxine therapy. Nevertheless, this patient showed reduced incorporation of 14C-labelled delta-aminolaevulinic acid into bone marrow haem. It is concluded that both congenital and primary acquired sideroblastic anaemia are a heterogenous group of disorders, with different defects in haem synthesis, reduced activity of delta-aminolaevulinic acid synthetase being common but not invariable in both types. Pyridoxine-responsive (congenital) sideroblastic anaemia may be due to the presence of an abnormal ALA-synthetase apoenzyme which requires excessive amounts of the coenzyme, pyridoxal-phosphate, to achieve normal activity; other abnormalities of haem synthesis may also be present."}
{"id": "PMID:465362", "title": "Altered erythrocyte pyrimidine activity in vitamin B12 or folate deficiency.", "content": "The net activity of orotidylic pyrophosphorylase and decarboxylase, sequential enzymes which catalyse the formation of uridine monophosphate from orotic acid in de novo pyrimidine biosynthesis, has been evaluated in erythrocytes of patients with folate or cobalamin deficiency. In patients with normoblastic haemopoiesis and normal cobalamin and folate status a direct relationship exists between the maturity of the peripheral blood erythrocyte population, as indicated by G6PD activity, and net orotidylic activity. In contrast in cobalamin or folate deficiency this co-ordinate relationship is not observed and net orotidylic activity is relatively reduced. Fractionation of erythrocytes by centrifugation demonstrates that this inordinately low orotidylic activity consistently occurs in the young erythrocyte population and is reversed by specific replacement therapy. In vitamin B12 or folate deficiency an impressive array of evidence now exists to implicate altered folate metabolism for the observed alterations in purine and pyrimidine metabolism (Das & Herbert, 1976). Of these changes the cornerstone defect resulting in megaloblastic maturation is impaired methylation of deoxyuridine monophosphate to thymidine monophosphate (Hoffbrand et al, 1976). In this context the reduced serum uridine plus uracil levels in patients with vitamin B12 deficiency (Parry & Blackmore, 1976) and the haematological response of these patients to orotic acid therapy (Rundles & Brewer, 1958) are not readily explicable. Since the conversion of orotic acid to uridine monophosphate depends on the integrity of the coupled activities of orotidylic pyrophosphorylase and decarboxylase, this study has quantitated this capacity in peripheral blood erythrocytes in patients with vitamin B12 or folate deficiency.", "contents": "Altered erythrocyte pyrimidine activity in vitamin B12 or folate deficiency. The net activity of orotidylic pyrophosphorylase and decarboxylase, sequential enzymes which catalyse the formation of uridine monophosphate from orotic acid in de novo pyrimidine biosynthesis, has been evaluated in erythrocytes of patients with folate or cobalamin deficiency. In patients with normoblastic haemopoiesis and normal cobalamin and folate status a direct relationship exists between the maturity of the peripheral blood erythrocyte population, as indicated by G6PD activity, and net orotidylic activity. In contrast in cobalamin or folate deficiency this co-ordinate relationship is not observed and net orotidylic activity is relatively reduced. Fractionation of erythrocytes by centrifugation demonstrates that this inordinately low orotidylic activity consistently occurs in the young erythrocyte population and is reversed by specific replacement therapy. In vitamin B12 or folate deficiency an impressive array of evidence now exists to implicate altered folate metabolism for the observed alterations in purine and pyrimidine metabolism (Das & Herbert, 1976). Of these changes the cornerstone defect resulting in megaloblastic maturation is impaired methylation of deoxyuridine monophosphate to thymidine monophosphate (Hoffbrand et al, 1976). In this context the reduced serum uridine plus uracil levels in patients with vitamin B12 deficiency (Parry & Blackmore, 1976) and the haematological response of these patients to orotic acid therapy (Rundles & Brewer, 1958) are not readily explicable. Since the conversion of orotic acid to uridine monophosphate depends on the integrity of the coupled activities of orotidylic pyrophosphorylase and decarboxylase, this study has quantitated this capacity in peripheral blood erythrocytes in patients with vitamin B12 or folate deficiency."}
{"id": "PMID:465363", "title": "Mechanism of the decreased erythropoiesis in the water deprived rat.", "content": "Radioiron uptake by erythrocytes, metabolic rate, erythropoietin formation during hypoxia and erythroid responsiveness to exogenous erythropoietin were determined in both starved and water deprived rats. The feed intake showed a marked and progressive reduction during water deprivation. The metabolic rates of rats deprived of either food of water declined progressively showing a 40% reduction 5d after water deprivation or starvation began. At this time, the 24 h red blood cells 59Fe incorporation was 85% lower in both starved and dehydrated rats than in normal rats. Plasma erythropoietin levels in response to hypoxia were approximately 50% decreased in both starved and dehydrated rats. Both polycythaemic starved and polycythaemic water deprived rats injected with human urinary erythropoietin showed a 75% decrease in 59Fe incorporation into erythrocytes when compared to control rats. It is suggested that depression of erythropoiesis during water deprivation in the rat depends on a reduced sensitivity to erythropoietin, possibly associated with decreased production of the hormone. Since water deprived rats drastically reduce feed intake it is suggested that secondary starvation is the principal cause of the decreased erythropoiesis induced in the rat by water deprivation.", "contents": "Mechanism of the decreased erythropoiesis in the water deprived rat. Radioiron uptake by erythrocytes, metabolic rate, erythropoietin formation during hypoxia and erythroid responsiveness to exogenous erythropoietin were determined in both starved and water deprived rats. The feed intake showed a marked and progressive reduction during water deprivation. The metabolic rates of rats deprived of either food of water declined progressively showing a 40% reduction 5d after water deprivation or starvation began. At this time, the 24 h red blood cells 59Fe incorporation was 85% lower in both starved and dehydrated rats than in normal rats. Plasma erythropoietin levels in response to hypoxia were approximately 50% decreased in both starved and dehydrated rats. Both polycythaemic starved and polycythaemic water deprived rats injected with human urinary erythropoietin showed a 75% decrease in 59Fe incorporation into erythrocytes when compared to control rats. It is suggested that depression of erythropoiesis during water deprivation in the rat depends on a reduced sensitivity to erythropoietin, possibly associated with decreased production of the hormone. Since water deprived rats drastically reduce feed intake it is suggested that secondary starvation is the principal cause of the decreased erythropoiesis induced in the rat by water deprivation."}
{"id": "PMID:465364", "title": "Erythrokinetic studies in hairy-cell leukaemia.", "content": "Erythrokinetics were studied in 29 patients with hairy-cell leukaemia. In all cases there was an increase in plasma volume, closely correlated to the size of the spleen, indicating that the true degree of anaemia can only be appreciated by red cell volume measurement. Moderately increased haemolysis was observed in most cases, which did not correlate with the spleen size. Simultaneous study of autologous and isologous red cell life-span suggested an extra-corpuscular mechanism for the haemolysis in most patients. A quantitative erythropoietic defect, either relative or absolute, was found in half the cases, without any qualitative defect. Only one case showed erythroid metaplasia of the spleen. Thus marrow failure appears to be largely responsible for the anaemia and granulocytopenia in hairy-cell leukaemia. A clear correlation was shown between the short-term prognosis after splenectomy and the degree of hypersplenism. However, long-term survival correlated chiefly with the degree of bone marrow failure, whether splenectomy had been carried out or not. The results indicate that isotope studies in hairy-cell leukaemia are useful both in determining the best form of treatment and predicting survival.", "contents": "Erythrokinetic studies in hairy-cell leukaemia. Erythrokinetics were studied in 29 patients with hairy-cell leukaemia. In all cases there was an increase in plasma volume, closely correlated to the size of the spleen, indicating that the true degree of anaemia can only be appreciated by red cell volume measurement. Moderately increased haemolysis was observed in most cases, which did not correlate with the spleen size. Simultaneous study of autologous and isologous red cell life-span suggested an extra-corpuscular mechanism for the haemolysis in most patients. A quantitative erythropoietic defect, either relative or absolute, was found in half the cases, without any qualitative defect. Only one case showed erythroid metaplasia of the spleen. Thus marrow failure appears to be largely responsible for the anaemia and granulocytopenia in hairy-cell leukaemia. A clear correlation was shown between the short-term prognosis after splenectomy and the degree of hypersplenism. However, long-term survival correlated chiefly with the degree of bone marrow failure, whether splenectomy had been carried out or not. The results indicate that isotope studies in hairy-cell leukaemia are useful both in determining the best form of treatment and predicting survival."}
{"id": "PMID:465365", "title": "Clinical staging in multiple myeloma.", "content": "Analysis of 237 patients with myeloma, retrospectively classified according to the staging system proposed by Durie and Salmon, showed highly significant differences (P less than 0.0001) in the survival of patients with low, intermediate and high tumour cell masses (Stages I, II and III respectively). The median survival for Stage I patients was 64 months, Stage II 32 months, and Stage III 6 months. The median survival of patients presenting with renal impairment was only 2 months, compared to 21 months for those presenting with normal renal function (P less than 0.0001). The use of this clinical staging system, which is based on myeloma protein synthesis rates and the calculation of total body tumour cell mass, should be considered in planning future trials of therapy in this disease.", "contents": "Clinical staging in multiple myeloma. Analysis of 237 patients with myeloma, retrospectively classified according to the staging system proposed by Durie and Salmon, showed highly significant differences (P less than 0.0001) in the survival of patients with low, intermediate and high tumour cell masses (Stages I, II and III respectively). The median survival for Stage I patients was 64 months, Stage II 32 months, and Stage III 6 months. The median survival of patients presenting with renal impairment was only 2 months, compared to 21 months for those presenting with normal renal function (P less than 0.0001). The use of this clinical staging system, which is based on myeloma protein synthesis rates and the calculation of total body tumour cell mass, should be considered in planning future trials of therapy in this disease."}
{"id": "PMID:465366", "title": "Splenectomy in patients with agnogenic myeloid metaplasia: an analysis of 321 published cases.", "content": "The available data on 321 published cases of splenectomy in agnogenic myeloid metaplasia were reviewed. Anaemia and thrombopenia were the most common indications for this operation. Patients reported after 1940 had a median postoperative survival of 13 months. Their postoperative mortality was 7.5% after 2 d and 25.7% after 3 months. The postoperative mortality was significantly higher when the weight of the spleen exceeded 2000 g, or in patients with platelet count less than 70 X 10(9)/l. The most common causes of death were infections (34.4%), cardiac or thromboembolic events (26.4%) and haemorrhage (17.6%). Leukaemic transformation was reported in 11.2% of the cases.", "contents": "Splenectomy in patients with agnogenic myeloid metaplasia: an analysis of 321 published cases. The available data on 321 published cases of splenectomy in agnogenic myeloid metaplasia were reviewed. Anaemia and thrombopenia were the most common indications for this operation. Patients reported after 1940 had a median postoperative survival of 13 months. Their postoperative mortality was 7.5% after 2 d and 25.7% after 3 months. The postoperative mortality was significantly higher when the weight of the spleen exceeded 2000 g, or in patients with platelet count less than 70 X 10(9)/l. The most common causes of death were infections (34.4%), cardiac or thromboembolic events (26.4%) and haemorrhage (17.6%). Leukaemic transformation was reported in 11.2% of the cases."}
{"id": "PMID:465367", "title": "Oxidative damage to neutrophils in glutathione synthetase deficiency.", "content": "Several episodes of neutropenia were observed in a child with glutathione synthetase deficiency (5-oxoprolinuria). Studies of the patient's glutathione-deficient neutrophils were undertaken to examine the responses of the cells to oxidative stress associated with phagocytosis. The patient's neutrophils contained 10--20% of normal glutathione content. Circulating neutrophils in infection-free periods appeared less mature than normal by morphologic criteria, suggesting increased cell turnover. The cells ingested particles, responded to chemotactic stimuli, and oxidized 1-14C glucose normally. However, following ingestion of particles, the cells accumulated excess hydrogen peroxide compared with normal cells, and showed impaired protein iodination and bacterial killing. Electron micrographs revealed damage to microtubules and membranous structures in the patient's neutrophils during phagocytosis. The level of glutathione in the cells appears inadequate to protect against peroxide generated during normal cell function, and the cells are thus damaged and rendered less effective in bacterial killing. The data provide evidence for a protective role of glutathione in normal neutrophil function.", "contents": "Oxidative damage to neutrophils in glutathione synthetase deficiency. Several episodes of neutropenia were observed in a child with glutathione synthetase deficiency (5-oxoprolinuria). Studies of the patient's glutathione-deficient neutrophils were undertaken to examine the responses of the cells to oxidative stress associated with phagocytosis. The patient's neutrophils contained 10--20% of normal glutathione content. Circulating neutrophils in infection-free periods appeared less mature than normal by morphologic criteria, suggesting increased cell turnover. The cells ingested particles, responded to chemotactic stimuli, and oxidized 1-14C glucose normally. However, following ingestion of particles, the cells accumulated excess hydrogen peroxide compared with normal cells, and showed impaired protein iodination and bacterial killing. Electron micrographs revealed damage to microtubules and membranous structures in the patient's neutrophils during phagocytosis. The level of glutathione in the cells appears inadequate to protect against peroxide generated during normal cell function, and the cells are thus damaged and rendered less effective in bacterial killing. The data provide evidence for a protective role of glutathione in normal neutrophil function."}
{"id": "PMID:465368", "title": "Kinetics of red cell membrane phosphorylation: altered affinity of HS membrane protein acceptors.", "content": "Membrane ghosts were prepared from red blood cells of normal and hereditary spherocytosis (HS) subjects. Time dependent phosphorylation studies of the membrane (in the presence of gamma labelled AT32P) showed that the HS membrane appeared to incorporate less phosphate than the normal membrane but the results were not significantly different. Using the initial linear rate of phosphorylation and varying the ATP concentration, it was found that the Michaelis constant (Km) for the normal membrane was 24.8 microns ATP and Vmax was 0.120 nmol phosphate bound/mg membrane protein/min. These values for the HS membrane were found to be 39.0 and 0.118 respectively. In one family the affected mother and son each showed a positive cooperativity effect for similar reciprocal plots. This indicates that phosphorylation of various HS membrane proteins may be an ordered rather than random process and suggests biochemical heterogencity of the HS condition. The constants, Km and Vmax, however, were found to be similar to the rest of HS subjects studied. Crude extracts of the enzyme protein kinase, which catalyses the membrane phosphorylation, showed no significant difference in the Km and Vmax value in HS compared with that from normal red cells. The kinetic difference in phosphorylation is probably due to abnormalities in the membrane protein(s) which accept the phosphate from protein kinase in HS red cells.", "contents": "Kinetics of red cell membrane phosphorylation: altered affinity of HS membrane protein acceptors. Membrane ghosts were prepared from red blood cells of normal and hereditary spherocytosis (HS) subjects. Time dependent phosphorylation studies of the membrane (in the presence of gamma labelled AT32P) showed that the HS membrane appeared to incorporate less phosphate than the normal membrane but the results were not significantly different. Using the initial linear rate of phosphorylation and varying the ATP concentration, it was found that the Michaelis constant (Km) for the normal membrane was 24.8 microns ATP and Vmax was 0.120 nmol phosphate bound/mg membrane protein/min. These values for the HS membrane were found to be 39.0 and 0.118 respectively. In one family the affected mother and son each showed a positive cooperativity effect for similar reciprocal plots. This indicates that phosphorylation of various HS membrane proteins may be an ordered rather than random process and suggests biochemical heterogencity of the HS condition. The constants, Km and Vmax, however, were found to be similar to the rest of HS subjects studied. Crude extracts of the enzyme protein kinase, which catalyses the membrane phosphorylation, showed no significant difference in the Km and Vmax value in HS compared with that from normal red cells. The kinetic difference in phosphorylation is probably due to abnormalities in the membrane protein(s) which accept the phosphate from protein kinase in HS red cells."}
{"id": "PMID:465369", "title": "Nonrecessively transmitted nonspherocytic hereditary haemolytic anaemia associated with increased red cell glutathione.", "content": "Hereditary haemolytic anaemia not associated with spherocytosis, ovalocytosis, stomatocytosis or haemoglobinopathy was observed in three members of a kindred. There were no demonstrable abnormalities of enzymes of the Embden-Myerhof pathway, the hexosemonophosphate shunt, or of a variety of non-glycolytic enzymatic activities assayed in patient erythrocytes. In each case, red cell glutathione was increased in concentration three to six standard deviations above our normal mean. Erythrocyte glutathione was normally stable and all sulfhydryl reacting material was shown by specific enzyme assays to represent either reduced (97%) or oxidized (3%) glutathione. No abnormality in active transport of oxidized glutathione was demonstrable in the red cells. This syndrome of unknown actiology was transmitted in autosomally dominant fashion or, alternatively, as a possibly x-chromosome linked disorder. Existing data did not permit the differentiation of these two possibilities.", "contents": "Nonrecessively transmitted nonspherocytic hereditary haemolytic anaemia associated with increased red cell glutathione. Hereditary haemolytic anaemia not associated with spherocytosis, ovalocytosis, stomatocytosis or haemoglobinopathy was observed in three members of a kindred. There were no demonstrable abnormalities of enzymes of the Embden-Myerhof pathway, the hexosemonophosphate shunt, or of a variety of non-glycolytic enzymatic activities assayed in patient erythrocytes. In each case, red cell glutathione was increased in concentration three to six standard deviations above our normal mean. Erythrocyte glutathione was normally stable and all sulfhydryl reacting material was shown by specific enzyme assays to represent either reduced (97%) or oxidized (3%) glutathione. No abnormality in active transport of oxidized glutathione was demonstrable in the red cells. This syndrome of unknown actiology was transmitted in autosomally dominant fashion or, alternatively, as a possibly x-chromosome linked disorder. Existing data did not permit the differentiation of these two possibilities."}
{"id": "PMID:465370", "title": "Vitamin B6 metabolism in idiopathic sideroblastic anaemia and related disorders.", "content": "Patients with idiopathic anaemias associated with abnormal sideroblasts were defined according to morphologic and ferrokinetic criteria and the haematologic and biochemical effects of vitamin B6 therapy were evaluated. While all patients presented similar clinical pictures, peripheral blood changes and bone marrow abnormalities, two distinct groups were identified by sideroblast morphology and ferrokinetics. Patients with more than 5% true ring sideroblasts in the marrow (IRSA) uniformly had marked ineffective erythropoiesis, while those with abnormal sideroblasts but few true ring forms were hypoproliferative. Measurements of red cell pyridoxine kinase (PnK) and intracellular pyridoxal 5-phosphate availability (PLP) as assessed by the activity of the PLP-dependent enzyme asparate aminotransferase (EGOT), revealed slightly decreased PnK levels in IRSA subjects but normal intracellular PLP activities in both groups. Furthermore, when treated with pyridoxine, all patients showed increases in both red cell PnK and EGOT activities which were similar to those seen in normal subjects. Treatment with PLP also effectively increased erythrocyte vitamin B6 activity. Even so, neither pyridoxine nor intramuscular PLP improved erythropoiesis as determined by serial haematocrits, reticulocyte counts and erythron iron turnover measurements.However, since both therapies increased red cell protoporphyrin levels and the excretion of urinary coproporphyrin in a number of subjects, the possibility that impaired haem synthesis in the sideroblastic anaemias is associated with abnormal vitamin B6 metabolism at the level of the mitochondrion must still be considered.", "contents": "Vitamin B6 metabolism in idiopathic sideroblastic anaemia and related disorders. Patients with idiopathic anaemias associated with abnormal sideroblasts were defined according to morphologic and ferrokinetic criteria and the haematologic and biochemical effects of vitamin B6 therapy were evaluated. While all patients presented similar clinical pictures, peripheral blood changes and bone marrow abnormalities, two distinct groups were identified by sideroblast morphology and ferrokinetics. Patients with more than 5% true ring sideroblasts in the marrow (IRSA) uniformly had marked ineffective erythropoiesis, while those with abnormal sideroblasts but few true ring forms were hypoproliferative. Measurements of red cell pyridoxine kinase (PnK) and intracellular pyridoxal 5-phosphate availability (PLP) as assessed by the activity of the PLP-dependent enzyme asparate aminotransferase (EGOT), revealed slightly decreased PnK levels in IRSA subjects but normal intracellular PLP activities in both groups. Furthermore, when treated with pyridoxine, all patients showed increases in both red cell PnK and EGOT activities which were similar to those seen in normal subjects. Treatment with PLP also effectively increased erythrocyte vitamin B6 activity. Even so, neither pyridoxine nor intramuscular PLP improved erythropoiesis as determined by serial haematocrits, reticulocyte counts and erythron iron turnover measurements.However, since both therapies increased red cell protoporphyrin levels and the excretion of urinary coproporphyrin in a number of subjects, the possibility that impaired haem synthesis in the sideroblastic anaemias is associated with abnormal vitamin B6 metabolism at the level of the mitochondrion must still be considered."}
{"id": "PMID:465371", "title": "Ferritin as a cytosol iron transport intermediate in human reticulocytes.", "content": "The major iron-bearing cytosol components of human reticulocytes identified after incubation with 59 Fe-125I-transferrin have been studied further. Component C previously found to behave consistently as an intermediate in the iron transport pathway to haem is shown to consist entirely of ferritin. After a short pulse of labelled transferrin incubation, chase experiments showed a fall of ferritin label with time and a corresponding increase in haemoglobin-iron incorporation. There was no loss of ferritin to the culture medium. Restriction of iron uptake by reticulocytes using both p-hydroxymercuribenzoate inhibition of uptake and incubation with progressively lower saturations of iron-transferrin gave linearly related incorporation of 59Fe into ferritin and haemoglobin at all levels of iron uptake, thus negating the concept of ferritin as an 'overspill' form of reticulocyte iron. The results suggest that cytosol ferritin is an obligatory intermediate in reticulocyte iron transport.", "contents": "Ferritin as a cytosol iron transport intermediate in human reticulocytes. The major iron-bearing cytosol components of human reticulocytes identified after incubation with 59 Fe-125I-transferrin have been studied further. Component C previously found to behave consistently as an intermediate in the iron transport pathway to haem is shown to consist entirely of ferritin. After a short pulse of labelled transferrin incubation, chase experiments showed a fall of ferritin label with time and a corresponding increase in haemoglobin-iron incorporation. There was no loss of ferritin to the culture medium. Restriction of iron uptake by reticulocytes using both p-hydroxymercuribenzoate inhibition of uptake and incubation with progressively lower saturations of iron-transferrin gave linearly related incorporation of 59Fe into ferritin and haemoglobin at all levels of iron uptake, thus negating the concept of ferritin as an 'overspill' form of reticulocyte iron. The results suggest that cytosol ferritin is an obligatory intermediate in reticulocyte iron transport."}
{"id": "PMID:465372", "title": "The influence of red blood cells on the effects of aspirin or sulphinpyrazone on platelet adherence to damaged rabbit aorta.", "content": "The effects of acetylsalicylic acid (ASA; aspirin) or sulphinpyrazone (SP) on the adherence of washed rabbit platelets to the subendotheilial surface of an everted aorta mounted on a probe or to the subendothelial surface of a rabbit aorta attached to a perfusion apparatus were examined. ASA had no effect on platelet adherence to a damaged aorta perfused with a suspension of washed platelets in a medium containing 10% red blood cells (RBC); SP was slightly inhibitory at high concentration. When damaged rabbit aortae were everted on a probe and rotated in a suspension of washed platelets to which RBC were added to a packed cell volume of 10%, both ASA and SP inhibited platelet adherence to the damaged vessel wall. When the PCV was 40%, ASA was not inhibitory and SP reduced platelet adherence only at very high concentrations. It is therefore unlikely that, at the concentrations achieved in man, SP exerts an effect on platelet adherence. The different effects of ASA and SP on platelet survival do not appear attributable to their effects on platelet adherence.", "contents": "The influence of red blood cells on the effects of aspirin or sulphinpyrazone on platelet adherence to damaged rabbit aorta. The effects of acetylsalicylic acid (ASA; aspirin) or sulphinpyrazone (SP) on the adherence of washed rabbit platelets to the subendotheilial surface of an everted aorta mounted on a probe or to the subendothelial surface of a rabbit aorta attached to a perfusion apparatus were examined. ASA had no effect on platelet adherence to a damaged aorta perfused with a suspension of washed platelets in a medium containing 10% red blood cells (RBC); SP was slightly inhibitory at high concentration. When damaged rabbit aortae were everted on a probe and rotated in a suspension of washed platelets to which RBC were added to a packed cell volume of 10%, both ASA and SP inhibited platelet adherence to the damaged vessel wall. When the PCV was 40%, ASA was not inhibitory and SP reduced platelet adherence only at very high concentrations. It is therefore unlikely that, at the concentrations achieved in man, SP exerts an effect on platelet adherence. The different effects of ASA and SP on platelet survival do not appear attributable to their effects on platelet adherence."}
{"id": "PMID:465373", "title": "Detection of carriers of haemophilia B.", "content": "Plasma levels of factor IX activity and factor IX antigen were determined in 18 definite carriers of haemophilia B-, 10 definite carriers of haemophilia B+ and 40 control subjects. Factor IX antigen was determined by the electroimmunoassay technique of Laurell using a rabbit antiserum against factor IX. In haemophilia B-, statistical tolerance regions were constructed containing 90% of the factor IX activity and factor IX antigen values for the carriers and the control subjects. Of the 18 carriers, 10 were outside the tolerance region for the control subjects, and of the 40 control subjects, 17 were outside the tolerance region for the carriers. A better separation of the probabilities of being a carrier was obtained for the B- carriers and the control subjects when factor IX antigen was determined in addition to factor IX activity. In haemophilia B+, factor IX antigen was present in excess of factor IX activity in nine of the 10 carriers. The values for factor IX activity and factor IX antigen for these nine carriers fell outside the 90% tolerance region for the control subjects. It is concluded that the quantitative determination of factor IX antigen may be of value in the detection of carriers of both haemophilia B+ and haemophilia B-.", "contents": "Detection of carriers of haemophilia B. Plasma levels of factor IX activity and factor IX antigen were determined in 18 definite carriers of haemophilia B-, 10 definite carriers of haemophilia B+ and 40 control subjects. Factor IX antigen was determined by the electroimmunoassay technique of Laurell using a rabbit antiserum against factor IX. In haemophilia B-, statistical tolerance regions were constructed containing 90% of the factor IX activity and factor IX antigen values for the carriers and the control subjects. Of the 18 carriers, 10 were outside the tolerance region for the control subjects, and of the 40 control subjects, 17 were outside the tolerance region for the carriers. A better separation of the probabilities of being a carrier was obtained for the B- carriers and the control subjects when factor IX antigen was determined in addition to factor IX activity. In haemophilia B+, factor IX antigen was present in excess of factor IX activity in nine of the 10 carriers. The values for factor IX activity and factor IX antigen for these nine carriers fell outside the 90% tolerance region for the control subjects. It is concluded that the quantitative determination of factor IX antigen may be of value in the detection of carriers of both haemophilia B+ and haemophilia B-."}
{"id": "PMID:465374", "title": "Inhibition by phospholipids of haemolytic action of asbestos.", "content": "Haemolysis by asbestos fibres results from an increase in membrane permeability and not from rupture of red blood cells (RBC). The effect of chrysotile asbestos on RBC is at least partly, if not completely, attributable to lipid extraction and adsorption on to the fibres. This was suggested by the hyperbolic relationship between the haemolytic activity of chrysotile and the relative concentration of both chrysotile and RBC. Moreover, it was shown that pre-incubation of chrysotile with lipids, either as RBC membranes or with pure lipids in the form of liposomes, prevents haemolysis.", "contents": "Inhibition by phospholipids of haemolytic action of asbestos. Haemolysis by asbestos fibres results from an increase in membrane permeability and not from rupture of red blood cells (RBC). The effect of chrysotile asbestos on RBC is at least partly, if not completely, attributable to lipid extraction and adsorption on to the fibres. This was suggested by the hyperbolic relationship between the haemolytic activity of chrysotile and the relative concentration of both chrysotile and RBC. Moreover, it was shown that pre-incubation of chrysotile with lipids, either as RBC membranes or with pure lipids in the form of liposomes, prevents haemolysis."}
{"id": "PMID:465375", "title": "HLA antigen frequencies in flax byssinosis patients.", "content": "Not all workers exposed to flax dust contract byssinosis. It is not known what determines susceptibility or insusceptibility. This study is an attempt to establish whether the incidence of histocompatibility antigens is involved in susceptibility to the disease. Forty patients suffering from flax byssinosis were tissue-typed for HLA-A and -B antigens. HLA-B27 was significantly more common in the patients (22.5%) than in the controls (5.5%); P = 0.029 after correction for the number of antigens compared. HLA-A11 was present in twelve patients (30%) compared with 14% in the controls; after correction for the number of comparisons, this is not a statistically significant increase. Because HLA-B27, though significantly more common in flax byssinosis, is not necessary for its occurrence (77.5% of our patients did not have it), it is possible that the increase in the frequency of HLA-B27 is attributable to an association with other genes, perhaps those regulating the immune response or coding for antigens at other HLA loci.", "contents": "HLA antigen frequencies in flax byssinosis patients. Not all workers exposed to flax dust contract byssinosis. It is not known what determines susceptibility or insusceptibility. This study is an attempt to establish whether the incidence of histocompatibility antigens is involved in susceptibility to the disease. Forty patients suffering from flax byssinosis were tissue-typed for HLA-A and -B antigens. HLA-B27 was significantly more common in the patients (22.5%) than in the controls (5.5%); P = 0.029 after correction for the number of antigens compared. HLA-A11 was present in twelve patients (30%) compared with 14% in the controls; after correction for the number of comparisons, this is not a statistically significant increase. Because HLA-B27, though significantly more common in flax byssinosis, is not necessary for its occurrence (77.5% of our patients did not have it), it is possible that the increase in the frequency of HLA-B27 is attributable to an association with other genes, perhaps those regulating the immune response or coding for antigens at other HLA loci."}
{"id": "PMID:465376", "title": "Mortality and morbidity in a pulp and a paper mill in the United States: a ten-year follow-up.", "content": "Two hundred and seventy-one men seen in 1963, who worked in a pulp and a paper mill, were followed up ten years later, in 1973. Death certificates were obtained for those who died. There did not appear to be any increased mortality in the group, nor was there any increased specific cause of death. A morbidity study of 200 men seen at both times did not show any differences in respiratory symptoms or prevalence of chronic non-specific respiratory disease. Analysis of pulmonary function showed little, if any, difference between groups. Analysis of retired, deceased, and still-working categories did suggest that exposures to Cl2 or SO2 might have a slight adverse effect on pulmonary function.", "contents": "Mortality and morbidity in a pulp and a paper mill in the United States: a ten-year follow-up. Two hundred and seventy-one men seen in 1963, who worked in a pulp and a paper mill, were followed up ten years later, in 1973. Death certificates were obtained for those who died. There did not appear to be any increased mortality in the group, nor was there any increased specific cause of death. A morbidity study of 200 men seen at both times did not show any differences in respiratory symptoms or prevalence of chronic non-specific respiratory disease. Analysis of pulmonary function showed little, if any, difference between groups. Analysis of retired, deceased, and still-working categories did suggest that exposures to Cl2 or SO2 might have a slight adverse effect on pulmonary function."}
{"id": "PMID:465377", "title": "Effects of tricresylphosphate on esterase activity of rat serum and tissues.", "content": "The effect of tricresylphosphate (TCP) was studied in vitro and in vivo on the rat liver and brain enzymes acetylcholinesterase (ACC), butyrylcholinesterase (CHE), arylesterase (ARE), aliesterase (ALI), and the microsomal nicotinamide-adenine dinucleotide phosphate oxidase (NADPH2-oxidase) system. The results show that, in the male rat, TCP given intraperitoneally induces an increase in liver microsomal ARE AND NADPH2-oxidase and a decrease in ALI and cholinesterase; no activation of ARE and NADPH2-oxidase is observed in female rats.", "contents": "Effects of tricresylphosphate on esterase activity of rat serum and tissues. The effect of tricresylphosphate (TCP) was studied in vitro and in vivo on the rat liver and brain enzymes acetylcholinesterase (ACC), butyrylcholinesterase (CHE), arylesterase (ARE), aliesterase (ALI), and the microsomal nicotinamide-adenine dinucleotide phosphate oxidase (NADPH2-oxidase) system. The results show that, in the male rat, TCP given intraperitoneally induces an increase in liver microsomal ARE AND NADPH2-oxidase and a decrease in ALI and cholinesterase; no activation of ARE and NADPH2-oxidase is observed in female rats."}
{"id": "PMID:465378", "title": "Cardiovascular disease and environmental exposure.", "content": "This paper reviews the possible association between cardiovascular disease and occupational and environmental agents. The effects of carbon monoxide, fibrogenic dusts, carbon disulphide, heavy metals, noise, radiation, heat, cold, solvents and fluorocarbons are discussed. New directions for investigation are suggested.", "contents": "Cardiovascular disease and environmental exposure. This paper reviews the possible association between cardiovascular disease and occupational and environmental agents. The effects of carbon monoxide, fibrogenic dusts, carbon disulphide, heavy metals, noise, radiation, heat, cold, solvents and fluorocarbons are discussed. New directions for investigation are suggested."}
{"id": "PMID:465380", "title": "Case note descriptions of the placenta: are they worthwhile?", "content": "In 429 placentae, measurements were made of weight, diameter, shape, eccentricity of the cord and weight and length of the cord, and the results were compared with Apgar score of the infant at birth and its standardized birth weight. There was no evidence that cord eccentricity, placental shape or \"thickness\", or the dimensions of the cord had any significant relation to the growth of the fetus or its condition at birth. The value of routine recording of crude measurements and qualitative assessments of the placenta in case notes is questioned.", "contents": "Case note descriptions of the placenta: are they worthwhile? In 429 placentae, measurements were made of weight, diameter, shape, eccentricity of the cord and weight and length of the cord, and the results were compared with Apgar score of the infant at birth and its standardized birth weight. There was no evidence that cord eccentricity, placental shape or \"thickness\", or the dimensions of the cord had any significant relation to the growth of the fetus or its condition at birth. The value of routine recording of crude measurements and qualitative assessments of the placenta in case notes is questioned."}
{"id": "PMID:465381", "title": "Induction of labour--a six year review.", "content": "A retrospective survey of surgical induction of labour has shown that induced patients had a lower perinatal mortality rate and a lower Caesarean section rate than non-induced women.", "contents": "Induction of labour--a six year review. A retrospective survey of surgical induction of labour has shown that induced patients had a lower perinatal mortality rate and a lower Caesarean section rate than non-induced women."}
{"id": "PMID:465382", "title": "Plasma HCG levels in patients with bleeding in the first and second trimesters of pregnancy.", "content": "The prognostic value of maternal plasma human chorionic gonadotrophin (hCG) determinations, measured by a specific beta-subunit radioimmunoassay, was studied in 188 patients with bleeding between 6 and 20 weeks gestation. The patients were arranged into different subgroups according to ultrasound findings as well as clinical and histopathological evidence. In threatened abortion with successful outcome (50 per cent of all patients studied), the weekly mean hCG values were normal or even slightly elevated. In patients with a blighted ovum, the first hCG level measured was in the normal range in 34 per cent of the patients. In patients who aborted embryo with former life signs hCG values were generally normal before the abortion. The hCG levels were usually low in patients with incomplete abortion and ectopic pregnancy. An initially subnormal level of hCG was associated with a poor outcome of pregnancy in 92 per cent of patients. If the first hCG level was within the normal range the outcome of pregnancy was favourable in 79 per cent of patients.", "contents": "Plasma HCG levels in patients with bleeding in the first and second trimesters of pregnancy. The prognostic value of maternal plasma human chorionic gonadotrophin (hCG) determinations, measured by a specific beta-subunit radioimmunoassay, was studied in 188 patients with bleeding between 6 and 20 weeks gestation. The patients were arranged into different subgroups according to ultrasound findings as well as clinical and histopathological evidence. In threatened abortion with successful outcome (50 per cent of all patients studied), the weekly mean hCG values were normal or even slightly elevated. In patients with a blighted ovum, the first hCG level measured was in the normal range in 34 per cent of the patients. In patients who aborted embryo with former life signs hCG values were generally normal before the abortion. The hCG levels were usually low in patients with incomplete abortion and ectopic pregnancy. An initially subnormal level of hCG was associated with a poor outcome of pregnancy in 92 per cent of patients. If the first hCG level was within the normal range the outcome of pregnancy was favourable in 79 per cent of patients."}
{"id": "PMID:465383", "title": "Serum follicle stimulating hormone levels in human pregnancy.", "content": "We describe a specific radioimmunoassay for follicle stimulating hormone (FSH) and the finding of immunoreactive FSH in human pregnancy sera. A total of 394 serum samples was taken from 204 normal pregnant women at different stages of gestation. The FSH levels remained constant throughout pregnancy and no rise was associated with the late first trimester elevation of human chorionic gonadotrophin (hCG).", "contents": "Serum follicle stimulating hormone levels in human pregnancy. We describe a specific radioimmunoassay for follicle stimulating hormone (FSH) and the finding of immunoreactive FSH in human pregnancy sera. A total of 394 serum samples was taken from 204 normal pregnant women at different stages of gestation. The FSH levels remained constant throughout pregnancy and no rise was associated with the late first trimester elevation of human chorionic gonadotrophin (hCG)."}
{"id": "PMID:465384", "title": "Red cell mass during and after normal pregnancy.", "content": "Red cell mass, derived from plasma volume and haematocrit, was calculated serially in 45 healthy women during pregnancy and 4 to 6 months after delivery. Compared to the non-pregnant, postpartum value there was a reduction of approximately 100 ml at 12 weeks gestation. An increase in red cell mass occurred thereafter: at 36 weeks gestation values 180 ml greater than the non-pregnant were found. Iron and folic acid supplements almost doubled this increment to 349 ml of red cells.", "contents": "Red cell mass during and after normal pregnancy. Red cell mass, derived from plasma volume and haematocrit, was calculated serially in 45 healthy women during pregnancy and 4 to 6 months after delivery. Compared to the non-pregnant, postpartum value there was a reduction of approximately 100 ml at 12 weeks gestation. An increase in red cell mass occurred thereafter: at 36 weeks gestation values 180 ml greater than the non-pregnant were found. Iron and folic acid supplements almost doubled this increment to 349 ml of red cells."}
{"id": "PMID:465385", "title": "In vitro study of the possible role of the intrauterine contraceptive device tail in ascending infection of the genital tract.", "content": "Potentially pathogenic bacteria colonized the mucus coating the tails of both monofilamentous and multifilamentous intrauterine contraceptive devices (IUCD) in 72 (55 per cent) out of 132 in vitro tests. This suggests that the IUCD tail may be responsible for the passage of vaginal bacteria into the uterus.", "contents": "In vitro study of the possible role of the intrauterine contraceptive device tail in ascending infection of the genital tract. Potentially pathogenic bacteria colonized the mucus coating the tails of both monofilamentous and multifilamentous intrauterine contraceptive devices (IUCD) in 72 (55 per cent) out of 132 in vitro tests. This suggests that the IUCD tail may be responsible for the passage of vaginal bacteria into the uterus."}
{"id": "PMID:465386", "title": "Artificial insemination in Denmark by frozen donor semen supplied from a central bank.", "content": "A central semen bank in Denmark supplied deep-frozen semen. Selection of donors, freezing technique, and practical details about transport of specimens are described. In one of the gynaecological departments supplied with frozen semen, 22 out of 30 patients who had artificial insemination became pregnant. The advantages and disadvantages of using frozen semen are discussed.", "contents": "Artificial insemination in Denmark by frozen donor semen supplied from a central bank. A central semen bank in Denmark supplied deep-frozen semen. Selection of donors, freezing technique, and practical details about transport of specimens are described. In one of the gynaecological departments supplied with frozen semen, 22 out of 30 patients who had artificial insemination became pregnant. The advantages and disadvantages of using frozen semen are discussed."}
{"id": "PMID:465387", "title": "Isolated arteritis of the cervix uteri.", "content": "Four patients with arteritis apparently isolated to the cervix uteri are presented and the clinical and pathological significance of this condition is discussed.", "contents": "Isolated arteritis of the cervix uteri. Four patients with arteritis apparently isolated to the cervix uteri are presented and the clinical and pathological significance of this condition is discussed."}
{"id": "PMID:465388", "title": "Malignant change in benign cystic teratoma of the ovary.", "content": "In a clinico-pathological study of malignant disease of the ovary in the former North East Metropolitan Region, malignant transformation of benign cystic teratoma has emerged as the commonest malignancy of \"germ cell\" origin. The clinical and pathological details of 12 patients are reported.", "contents": "Malignant change in benign cystic teratoma of the ovary. In a clinico-pathological study of malignant disease of the ovary in the former North East Metropolitan Region, malignant transformation of benign cystic teratoma has emerged as the commonest malignancy of \"germ cell\" origin. The clinical and pathological details of 12 patients are reported."}
{"id": "PMID:465389", "title": "Mixed mesenchymal sarcoma of the broad ligament: case report.", "content": "A 55-year-old patient who presented with a pelvic mass was found to have a mixed mesenchymal sarcoma of the broad ligament. The histogenesis and diagnostic criteria of this rare tumour are discussed.", "contents": "Mixed mesenchymal sarcoma of the broad ligament: case report. A 55-year-old patient who presented with a pelvic mass was found to have a mixed mesenchymal sarcoma of the broad ligament. The histogenesis and diagnostic criteria of this rare tumour are discussed."}
{"id": "PMID:465390", "title": "Idiopathic retroperitoneal fibrosis involving the vagina: case report.", "content": "A patient with idiopathic retroperitoneal fibrosis involving the vagina and causing urinary symptoms is described. The condition responded satisfactorily to immunosuppressive drugs and steroid.", "contents": "Idiopathic retroperitoneal fibrosis involving the vagina: case report. A patient with idiopathic retroperitoneal fibrosis involving the vagina and causing urinary symptoms is described. The condition responded satisfactorily to immunosuppressive drugs and steroid."}
{"id": "PMID:465391", "title": "Pseudo-obstruction of the bowel in pregnancy: case reports.", "content": "Two patients with intestinal obstruction in pregnancy are presented and discussed. In neither case was there any obvious cause for the intestianl obstruction. Pseudo-obstruction of the bowel has not previously been described as a complication of pregnancy.", "contents": "Pseudo-obstruction of the bowel in pregnancy: case reports. Two patients with intestinal obstruction in pregnancy are presented and discussed. In neither case was there any obvious cause for the intestianl obstruction. Pseudo-obstruction of the bowel has not previously been described as a complication of pregnancy."}
{"id": "PMID:465392", "title": "Placental intervillous blood flow measured by inhaled 133Xe clearance in relation to induction of epidural analgesia.", "content": "A method of determining placental intervillous blood flow (IBF) using an inhaled 133Xe technique is described. The method was used to investigate the effect on IBF of epidural analgesia with an initial dose of 37.5 mg bupivacaine. There was no statistically significant reduction in IBF following the initial dose of bupivacaine in spite of a small but statistically significant reduction in mean blood pressure. There was no significant correlation between the change in mean blood pressure and the percentage change in IBF following epidural block although the single largest percentage reduction in IBF was associated with the largest fall in mean blood pressure.", "contents": "Placental intervillous blood flow measured by inhaled 133Xe clearance in relation to induction of epidural analgesia. A method of determining placental intervillous blood flow (IBF) using an inhaled 133Xe technique is described. The method was used to investigate the effect on IBF of epidural analgesia with an initial dose of 37.5 mg bupivacaine. There was no statistically significant reduction in IBF following the initial dose of bupivacaine in spite of a small but statistically significant reduction in mean blood pressure. There was no significant correlation between the change in mean blood pressure and the percentage change in IBF following epidural block although the single largest percentage reduction in IBF was associated with the largest fall in mean blood pressure."}
{"id": "PMID:465393", "title": "Fetal breathing patterns in intrauterine growth retardation.", "content": "Fetal breathing movements were studied with real time ultrasound in 21 normal fetuses and 30 fetuses born small-for-dates (less than the 5th centile). The events of the study period were recorded on punched paper tape which was subjected to computer analysis. In the normal group the percentage time of breathing was 54 +/- 12.7 (mean +/- SD). The breath to breath interval was 1.34 +/- 0.25 seconds and the coefficient of variation of this interval was 49.6 +/- 7.7 per cent. In 17 of the small-for-dates group amount of breathing was reduced with short breathing episodes and long apnoea intervals. In the other 13 patients in the small-for-dates group the coefficient of variation for the breath to breath interval was significantly reduced (39.6 +/- 7.9 per cent, p less than 0.01).", "contents": "Fetal breathing patterns in intrauterine growth retardation. Fetal breathing movements were studied with real time ultrasound in 21 normal fetuses and 30 fetuses born small-for-dates (less than the 5th centile). The events of the study period were recorded on punched paper tape which was subjected to computer analysis. In the normal group the percentage time of breathing was 54 +/- 12.7 (mean +/- SD). The breath to breath interval was 1.34 +/- 0.25 seconds and the coefficient of variation of this interval was 49.6 +/- 7.7 per cent. In 17 of the small-for-dates group amount of breathing was reduced with short breathing episodes and long apnoea intervals. In the other 13 patients in the small-for-dates group the coefficient of variation for the breath to breath interval was significantly reduced (39.6 +/- 7.9 per cent, p less than 0.01)."}
{"id": "PMID:465394", "title": "Birth weight: analysis of variance and the linear additive model.", "content": "The normal distribution of birth weight in a Queensland population has been surveyed. A method of analysis is presented which allows for the influence of a number of factors that affect birth weight.", "contents": "Birth weight: analysis of variance and the linear additive model. The normal distribution of birth weight in a Queensland population has been surveyed. A method of analysis is presented which allows for the influence of a number of factors that affect birth weight."}
{"id": "PMID:465395", "title": "Birth weight of Hausa infants in Northern Nigeria.", "content": "The birth weight of 1460 Hausa singleton infants was recorded and analysed. The birth weight of Hausa infants (Mean +/- SD) was 3.03 +/- 0.45 kg, being higher in males (3.08 +/- 0.47 kg) than females (2.97 +/- 0.48 kg). The difference was significant (P less than 0.001). The mean birth weight of first born children was significantly lower than the subsequent born, and increasing birth order was associated with higher birth weight irrespective of maternal age. The birth weight was influenced more by the parity as compared to maternal age. The incidence of low birth weight was 15.8 per cent, being lower in males (13.6 per cent) as compared to females (18.1 per cent). Comparison were made with studies from other parts of the country as well as from other countries.", "contents": "Birth weight of Hausa infants in Northern Nigeria. The birth weight of 1460 Hausa singleton infants was recorded and analysed. The birth weight of Hausa infants (Mean +/- SD) was 3.03 +/- 0.45 kg, being higher in males (3.08 +/- 0.47 kg) than females (2.97 +/- 0.48 kg). The difference was significant (P less than 0.001). The mean birth weight of first born children was significantly lower than the subsequent born, and increasing birth order was associated with higher birth weight irrespective of maternal age. The birth weight was influenced more by the parity as compared to maternal age. The incidence of low birth weight was 15.8 per cent, being lower in males (13.6 per cent) as compared to females (18.1 per cent). Comparison were made with studies from other parts of the country as well as from other countries."}
{"id": "PMID:465396", "title": "Biochemical changes of normal pregnancy and puerperium in Lagos, Nigeria.", "content": "A cross-sectional study is presented of biochemical changes in the third trimester of normal pregnancy and puerperium during the wet season in the tropical climate of Lagos, Nigeria. These changes are less marked in first than in subsequent pregnancies, and although qualitatively similar, in some respects they differ from those observed in the temperate climatic zone. The indications are that quantitative differences may exist which could be relevant to the management of pregnancy in the tropics.", "contents": "Biochemical changes of normal pregnancy and puerperium in Lagos, Nigeria. A cross-sectional study is presented of biochemical changes in the third trimester of normal pregnancy and puerperium during the wet season in the tropical climate of Lagos, Nigeria. These changes are less marked in first than in subsequent pregnancies, and although qualitatively similar, in some respects they differ from those observed in the temperate climatic zone. The indications are that quantitative differences may exist which could be relevant to the management of pregnancy in the tropics."}
{"id": "PMID:465397", "title": "Differences in albumin distribution and dynamics between toxaemic and non-toxaemic pregnant women.", "content": "By using least squares curve fitting techniques, double exponential curves were fitted to points representing the decay of plasma 131I-albumin after blood volume estimation of 48 pregnant women. Data from the fitted curves provided estimates of the distribution of albumin between plasma and extravascular compartments, exchange between the two and rate of albumin catabolism. The lower total extracellular albumin mass of a group of 23 severely toxaemic patients was shown to arise solely from the difference in plasma albumin mass between this group and the remaining 25 patients. Comparison of these results with those in the literature suggests that pregnant women have a higher proportion of their exchangeable albumin mass in the plasma compartment and a higher rate of albumin catabolism than non-pregnant women.", "contents": "Differences in albumin distribution and dynamics between toxaemic and non-toxaemic pregnant women. By using least squares curve fitting techniques, double exponential curves were fitted to points representing the decay of plasma 131I-albumin after blood volume estimation of 48 pregnant women. Data from the fitted curves provided estimates of the distribution of albumin between plasma and extravascular compartments, exchange between the two and rate of albumin catabolism. The lower total extracellular albumin mass of a group of 23 severely toxaemic patients was shown to arise solely from the difference in plasma albumin mass between this group and the remaining 25 patients. Comparison of these results with those in the literature suggests that pregnant women have a higher proportion of their exchangeable albumin mass in the plasma compartment and a higher rate of albumin catabolism than non-pregnant women."}
{"id": "PMID:465398", "title": "The possible relation between late pregnancy hypertension and 5-hydroxytryptamine levels in maternal blood.", "content": "The concentrations of the vasoactive substance, 5-hydroxytryptamine (5-HT), were estimated in whole blood samples from 12 pregnant women with hypertension in the third trimester. These were compared with a control group of 15 normotensive pregnant women of the same gestation. The hypertensive patients were admitted for observation and the whole blood 5-HT estimated again after four days bed rest: in all patients the blood pressure had, by this time, returned to within the normal range. No significant difference was found between the mean blood 5-HT concentrations of the hypertensive and the normotensive groups. A small, but not statistically significant, rise occurred in the hypertensive group after four days bed rest. Thus this study gave no evidence that 5-HT is implicated in hypertension in pregnancy.", "contents": "The possible relation between late pregnancy hypertension and 5-hydroxytryptamine levels in maternal blood. The concentrations of the vasoactive substance, 5-hydroxytryptamine (5-HT), were estimated in whole blood samples from 12 pregnant women with hypertension in the third trimester. These were compared with a control group of 15 normotensive pregnant women of the same gestation. The hypertensive patients were admitted for observation and the whole blood 5-HT estimated again after four days bed rest: in all patients the blood pressure had, by this time, returned to within the normal range. No significant difference was found between the mean blood 5-HT concentrations of the hypertensive and the normotensive groups. A small, but not statistically significant, rise occurred in the hypertensive group after four days bed rest. Thus this study gave no evidence that 5-HT is implicated in hypertension in pregnancy."}
{"id": "PMID:465400", "title": "Changes in coagulation factors in postmenopausal women on ethinyl oestradiol.", "content": "Coagulation factors II, VII, X, VII + X complex and anti-thrombin III were measured in 10 postmenopausal women before, during and after a four-month course of 20 microgram daily of ethinyl oestradiol, taken three weeks in four. Factors VII, X and VII + X complex rose significantly during treatment but only factor X levels were significantly elevated at the end of the four-month treatment period. There was no change in factor II or anti-thrombin III levels. These effects on coagulation factors during ethinyl oestradiol therapy are, in general, similar to those found during treatment with conjugated equine oestrogens and oestradiol valerate in a previous study from the same laboratory. It seems likely that equipotent doses of ethinyl oestradiol and 'natural' oestrogens have similar effects on these coagulation factors.", "contents": "Changes in coagulation factors in postmenopausal women on ethinyl oestradiol. Coagulation factors II, VII, X, VII + X complex and anti-thrombin III were measured in 10 postmenopausal women before, during and after a four-month course of 20 microgram daily of ethinyl oestradiol, taken three weeks in four. Factors VII, X and VII + X complex rose significantly during treatment but only factor X levels were significantly elevated at the end of the four-month treatment period. There was no change in factor II or anti-thrombin III levels. These effects on coagulation factors during ethinyl oestradiol therapy are, in general, similar to those found during treatment with conjugated equine oestrogens and oestradiol valerate in a previous study from the same laboratory. It seems likely that equipotent doses of ethinyl oestradiol and 'natural' oestrogens have similar effects on these coagulation factors."}
{"id": "PMID:465401", "title": "HLA antigens in glaucoma and ocular hypertension.", "content": "Forty-five white patients (32 with open-angle glaucoma and 13 with ocular hypertension) and 63 black patients (41 with open-angle glaucoma and 22 with ocular hypertension) were tissue typed for a total of 38 HLA antigens and the results compared to normal, unrelated, panels of 248 white donors and 150 black volunteers, respectively. No statistically significant differences with regard to the frequencies of 38 HLA antigens were detected among the various groups.", "contents": "HLA antigens in glaucoma and ocular hypertension. Forty-five white patients (32 with open-angle glaucoma and 13 with ocular hypertension) and 63 black patients (41 with open-angle glaucoma and 22 with ocular hypertension) were tissue typed for a total of 38 HLA antigens and the results compared to normal, unrelated, panels of 248 white donors and 150 black volunteers, respectively. No statistically significant differences with regard to the frequencies of 38 HLA antigens were detected among the various groups."}
{"id": "PMID:465402", "title": "Immunoglobulin concentrations in human tears in ocular diseases.", "content": "Immunoglobulin concentrations in human tears were determined in 165 patients with different eye diseases by a standard radial immunodiffusion method. IgA was present in all the samples in measurable quantity. The mean IgA values were significantly higher than the controls in patients with acute bacterial conjunctivitis, keratomalacia, corneal graft reaction, blepharoconjunctivitis, and acute keratoconjunctivitis. The values in the patients with vernal conjunctivitis, phlyctenular conjunctivitis, acute bacterial corneal ulcer, and acute endogenous uveitis were not significantly different from those in the controls. IgG could be detected in the majority of the samples but it was in measurable quantity in 18 samples. IgM could be detected in fewer samples. IgD was not detected in any of them. The study indicates that, whenever the immunoglobulin levels in tears are altered in diseased eyes, it is the IgA level that is predominantly altered and not the IgG level.", "contents": "Immunoglobulin concentrations in human tears in ocular diseases. Immunoglobulin concentrations in human tears were determined in 165 patients with different eye diseases by a standard radial immunodiffusion method. IgA was present in all the samples in measurable quantity. The mean IgA values were significantly higher than the controls in patients with acute bacterial conjunctivitis, keratomalacia, corneal graft reaction, blepharoconjunctivitis, and acute keratoconjunctivitis. The values in the patients with vernal conjunctivitis, phlyctenular conjunctivitis, acute bacterial corneal ulcer, and acute endogenous uveitis were not significantly different from those in the controls. IgG could be detected in the majority of the samples but it was in measurable quantity in 18 samples. IgM could be detected in fewer samples. IgD was not detected in any of them. The study indicates that, whenever the immunoglobulin levels in tears are altered in diseased eyes, it is the IgA level that is predominantly altered and not the IgG level."}
{"id": "PMID:465403", "title": "Cell mediated immunity in relation to cataract and cataract surgery.", "content": "Two groups of patients suffering from cataract were investigated by the lymphocyte stimulation test. One group was tested before and the other after cataract surgery. Human (total) lens crystallins and bovine alpha crystallin were used as the antigens. Lymphocytes of healthy persons showed practically no positive stimulation with either antigen. Of the patients tested before operation 38% reacted positively with lens crystallins and 15% with alpha crystallin. This difference in sensitisation suggests a leakage of lenticular antigens other than alpha crystallin out of the lens during cataract progression. Extracapsular lens extraction caused an increase in the number of patients reacting positively with lens crystallins and alpha crystallin as compared to patients not operated upon. Intracapsular lens extraction on the other hand resulted in less sensitisation to lens crystallins as compared to patients not operated upon.", "contents": "Cell mediated immunity in relation to cataract and cataract surgery. Two groups of patients suffering from cataract were investigated by the lymphocyte stimulation test. One group was tested before and the other after cataract surgery. Human (total) lens crystallins and bovine alpha crystallin were used as the antigens. Lymphocytes of healthy persons showed practically no positive stimulation with either antigen. Of the patients tested before operation 38% reacted positively with lens crystallins and 15% with alpha crystallin. This difference in sensitisation suggests a leakage of lenticular antigens other than alpha crystallin out of the lens during cataract progression. Extracapsular lens extraction caused an increase in the number of patients reacting positively with lens crystallins and alpha crystallin as compared to patients not operated upon. Intracapsular lens extraction on the other hand resulted in less sensitisation to lens crystallins as compared to patients not operated upon."}
{"id": "PMID:465404", "title": "Experimental posterior penetrating eye injury in the rabbit. I. Method of production and natural history.", "content": "A technique has been developed which produces an experimental posterior penetrating eye injury that reproducibly results in traction retinal detachment in rabbit eyes. The standard injury is an incision through the pars plana with vitreous prolapse and incarceration; the wound is then carefully closed with microsurgical techniques. It appears that blood in the vitreous is an essential factor in the development of vitreous traction and of traction retinal detachment. The model, which has been successfully transferred to Rhesus monkey eyes, is considered useful for further histological, electromicroscopical, electrophysiological, and ultrasound studies of posterior penetrating injuries of the eye. Our immediate goal is to assess in a controlled experiment whether pars plana vitrectomy can interrupt the sequence of events leading to traction retinal detachment after a posterior penetrating injury.", "contents": "Experimental posterior penetrating eye injury in the rabbit. I. Method of production and natural history. A technique has been developed which produces an experimental posterior penetrating eye injury that reproducibly results in traction retinal detachment in rabbit eyes. The standard injury is an incision through the pars plana with vitreous prolapse and incarceration; the wound is then carefully closed with microsurgical techniques. It appears that blood in the vitreous is an essential factor in the development of vitreous traction and of traction retinal detachment. The model, which has been successfully transferred to Rhesus monkey eyes, is considered useful for further histological, electromicroscopical, electrophysiological, and ultrasound studies of posterior penetrating injuries of the eye. Our immediate goal is to assess in a controlled experiment whether pars plana vitrectomy can interrupt the sequence of events leading to traction retinal detachment after a posterior penetrating injury."}
{"id": "PMID:465405", "title": "Experimental posterior penetrating eye injury in the rabbit. II. Histology of wound, vitreous, and retina.", "content": "The histological findings of the wound, the vitreous, and the retina in the rabbit eye with experimental posterior penetrating injury are described. Wound healing had just begun at 3 days after injury and was well established by 9 to 12 days. It involved proliferation of cells from the episclera and from the choroid. The progression to a fibrous ingrowth from the wound occurred only in eyes with blood in the vitreous. The intravitreal fibroblastic proliferation had begen at 6 days after injury and seemed to be derived from the choroid, the nonpigmented ciliary epithelium and, posteriorly, from the optic nervehead. During the development of retinal detachment the configuration of the peripheral and posterior retina, together with the orientation of vitreous strands, suggested the presence of vitreous traction. We postulate that the presence of contractile fibroblasts (myofibroblasts) in the vitreous may provide the mechanism for vitreous traction. The retinal detachments were also characterised by epiretinal and subretinal membranes, but these were not prominent. The end-stage appearance of a soft, shrunken eye with cyclitic membrane formation and retinal detachment resembles the outcome in many human eyes after severe penetrating injuries.", "contents": "Experimental posterior penetrating eye injury in the rabbit. II. Histology of wound, vitreous, and retina. The histological findings of the wound, the vitreous, and the retina in the rabbit eye with experimental posterior penetrating injury are described. Wound healing had just begun at 3 days after injury and was well established by 9 to 12 days. It involved proliferation of cells from the episclera and from the choroid. The progression to a fibrous ingrowth from the wound occurred only in eyes with blood in the vitreous. The intravitreal fibroblastic proliferation had begen at 6 days after injury and seemed to be derived from the choroid, the nonpigmented ciliary epithelium and, posteriorly, from the optic nervehead. During the development of retinal detachment the configuration of the peripheral and posterior retina, together with the orientation of vitreous strands, suggested the presence of vitreous traction. We postulate that the presence of contractile fibroblasts (myofibroblasts) in the vitreous may provide the mechanism for vitreous traction. The retinal detachments were also characterised by epiretinal and subretinal membranes, but these were not prominent. The end-stage appearance of a soft, shrunken eye with cyclitic membrane formation and retinal detachment resembles the outcome in many human eyes after severe penetrating injuries."}
{"id": "PMID:465406", "title": "One model of outflow damage.", "content": "The intraocular pressure, facilities of outflow, and Po/C ratios of 3 groups of eyes were compared. Group 1 consisted of 20 eyes at risk to the development of acute closed-angle glaucoma that had been treated with prophylactic pilocarpine for at least 8 years. Group 2 consisted of 20 eyes at risk that had received no treatment and had been followed up for at least 4 years. Group 3 comprised 20 eyes in which there was no evidence of glaucoma. There were highly significant differences between the 3 groups. The 60 eyes were then provoked with pilocarpine and phenylephrine; 90% of Group 1, 75% of Group 2, and none of Group 3 developed significant gonioscopic angle closure. These results suggest that asymptomatic partial angle closure was the cause of the observed changes and provide a naturally occurring model of one mechanism that can produce outflow damage without clinical symptoms.", "contents": "One model of outflow damage. The intraocular pressure, facilities of outflow, and Po/C ratios of 3 groups of eyes were compared. Group 1 consisted of 20 eyes at risk to the development of acute closed-angle glaucoma that had been treated with prophylactic pilocarpine for at least 8 years. Group 2 consisted of 20 eyes at risk that had received no treatment and had been followed up for at least 4 years. Group 3 comprised 20 eyes in which there was no evidence of glaucoma. There were highly significant differences between the 3 groups. The 60 eyes were then provoked with pilocarpine and phenylephrine; 90% of Group 1, 75% of Group 2, and none of Group 3 developed significant gonioscopic angle closure. These results suggest that asymptomatic partial angle closure was the cause of the observed changes and provide a naturally occurring model of one mechanism that can produce outflow damage without clinical symptoms."}
{"id": "PMID:465407", "title": "Mechanisms in ocular hypertension.", "content": "This paper investigates the hypothesis that intermittent partial angle closure is one of the causes of ocular hypertension. 139 eyes from 76 patients with ocular hypertension were provoked with pilocarpine and phenylephrine. Four distinct responses appeared. Firstly, in 39 eyes (from 24 patients, 32%) gonioscopic closure of part or all of the angle appeared. Secondly, in 30 eyes (from 19 patients, 25%) no angle closure occurred but there was a substantial pigment release into the aqueous. Thirdly, in 9 eyes (from 8 patients, 11%) both angle closure and pigment release occurred. Finally, in 61 eyes (from 36 patients, 59%) neither angle closure nor pigment release appeared. (Since the 2 eyes of a patient did not always behave in the same way, for example, 1 eye might develop angle closure and the other not, 1 patient may appear in 2 groups.) From the first group 1 eye from each patient was randomly chosen for iridectomy. A repeat provocative test at least 1 year later produced a significantly different result. It is considered that the evidence obtained in this study supports the hypothesis that intermittent partial angle closure is one of the causes of ocular hypertension.", "contents": "Mechanisms in ocular hypertension. This paper investigates the hypothesis that intermittent partial angle closure is one of the causes of ocular hypertension. 139 eyes from 76 patients with ocular hypertension were provoked with pilocarpine and phenylephrine. Four distinct responses appeared. Firstly, in 39 eyes (from 24 patients, 32%) gonioscopic closure of part or all of the angle appeared. Secondly, in 30 eyes (from 19 patients, 25%) no angle closure occurred but there was a substantial pigment release into the aqueous. Thirdly, in 9 eyes (from 8 patients, 11%) both angle closure and pigment release occurred. Finally, in 61 eyes (from 36 patients, 59%) neither angle closure nor pigment release appeared. (Since the 2 eyes of a patient did not always behave in the same way, for example, 1 eye might develop angle closure and the other not, 1 patient may appear in 2 groups.) From the first group 1 eye from each patient was randomly chosen for iridectomy. A repeat provocative test at least 1 year later produced a significantly different result. It is considered that the evidence obtained in this study supports the hypothesis that intermittent partial angle closure is one of the causes of ocular hypertension."}
{"id": "PMID:465408", "title": "Changes in refraction between the ages of 1 and 3 1/2 years.", "content": "A study has been made of the changes in refraction as a sample of 148 children grew between the ages of 1 and 3 1/2 years. There was no decrease in hypermetropia, but there was a significant decrease in the incidence of astigmatism. Study of the changes in the refraction in the horizontal and vertical meridia of individual eyes gave clear evidence of a trend towards emmetropia if the initial refraction in either meridian was myopic or less than +2.50 D. Above that level the refraction became more or less hypermetropic.", "contents": "Changes in refraction between the ages of 1 and 3 1/2 years. A study has been made of the changes in refraction as a sample of 148 children grew between the ages of 1 and 3 1/2 years. There was no decrease in hypermetropia, but there was a significant decrease in the incidence of astigmatism. Study of the changes in the refraction in the horizontal and vertical meridia of individual eyes gave clear evidence of a trend towards emmetropia if the initial refraction in either meridian was myopic or less than +2.50 D. Above that level the refraction became more or less hypermetropic."}
{"id": "PMID:465409", "title": "Refraction of 1-year-old children after atropine cycloplegia.", "content": "The refractions of 1648 children aged 11 to 13 months are reported. Atropine 1% was used for cycloplegia. 11.83% of the children had bilateral hypermetropia of +2.00 or more D. 13.23% of them had +1.50 or more D astigmatism in one or both eyes, and 6.5% had anisometropia. Anisometropia was significantly (P=0.000 001%) associated with bilateral hypermetropia, but even more significantly (P=0.000 000 4%) associated with astigmatism of +1.50 or more D in one or both eyes.", "contents": "Refraction of 1-year-old children after atropine cycloplegia. The refractions of 1648 children aged 11 to 13 months are reported. Atropine 1% was used for cycloplegia. 11.83% of the children had bilateral hypermetropia of +2.00 or more D. 13.23% of them had +1.50 or more D astigmatism in one or both eyes, and 6.5% had anisometropia. Anisometropia was significantly (P=0.000 001%) associated with bilateral hypermetropia, but even more significantly (P=0.000 000 4%) associated with astigmatism of +1.50 or more D in one or both eyes."}
{"id": "PMID:465410", "title": "Refraction of 1-year-old children after cycloplegia with 1% cyclopentolate: comparison with findings after atropinisation.", "content": "Cyclopentolate 1% is significantly less effective than atropine 1% at producing cycloplegia in 1-year-old children. If cycloplegic refraction is to be used for investigation or screening children for visual defects during the sensitive period, the more prolonged and profound cycloplegia following atropine could potentially have a disastrous effect on the development of vision. Cyclopentolate 1% would have to be used, and allowance made for its inadequacy as a cycloplegic.", "contents": "Refraction of 1-year-old children after cycloplegia with 1% cyclopentolate: comparison with findings after atropinisation. Cyclopentolate 1% is significantly less effective than atropine 1% at producing cycloplegia in 1-year-old children. If cycloplegic refraction is to be used for investigation or screening children for visual defects during the sensitive period, the more prolonged and profound cycloplegia following atropine could potentially have a disastrous effect on the development of vision. Cyclopentolate 1% would have to be used, and allowance made for its inadequacy as a cycloplegic."}
{"id": "PMID:465411", "title": "A simple method of detecting abnormal retinal correspondence.", "content": "A method is described of determining the state of retinal correspondence by means of an ordinary streak retinoscope used to produce a modified after-image test.", "contents": "A simple method of detecting abnormal retinal correspondence. A method is described of determining the state of retinal correspondence by means of an ordinary streak retinoscope used to produce a modified after-image test."}
{"id": "PMID:465412", "title": "Silicone oil in the treatment of massive preretinal retraction. I. Results in 105 eyes.", "content": "A study of 105 eyes in which intraocular injection of silicone oil was used to treat retinal detachment with massive preretinal retraction showed that early results were encouraging, and anatomical and visual results correlated well. Operative complications had little influence on the immediate outcome, the reasons for failure being poor case selection or inadequate surgical technique. When a deterioration occurred during the postoperative period it was found to be associated with late complications, particularly cataract; redetachment occurred in only a small number (12%) of cases.", "contents": "Silicone oil in the treatment of massive preretinal retraction. I. Results in 105 eyes. A study of 105 eyes in which intraocular injection of silicone oil was used to treat retinal detachment with massive preretinal retraction showed that early results were encouraging, and anatomical and visual results correlated well. Operative complications had little influence on the immediate outcome, the reasons for failure being poor case selection or inadequate surgical technique. When a deterioration occurred during the postoperative period it was found to be associated with late complications, particularly cataract; redetachment occurred in only a small number (12%) of cases."}
{"id": "PMID:465413", "title": "Silicone oil injection in the treatment of massive preretinal retraction. II. Late complications in 93 eyes.", "content": "Long-term assessment of eyes in which silicone oil injection had been used in the treatment of retinal detachment with massive preretinal retraction was undertaken in 92 consecutive patients. While a high incidence of complications, particularly cataract, was confirmed, this study showed that they were probably caused not by any toxic effect of silicone oil but by obstruction of normal metabolic exchange at the silicone-tissue interface. The incidence of complications causing deterioration of vision or serious symptoms was not found to be high, and navigating vision was well preserved.", "contents": "Silicone oil injection in the treatment of massive preretinal retraction. II. Late complications in 93 eyes. Long-term assessment of eyes in which silicone oil injection had been used in the treatment of retinal detachment with massive preretinal retraction was undertaken in 92 consecutive patients. While a high incidence of complications, particularly cataract, was confirmed, this study showed that they were probably caused not by any toxic effect of silicone oil but by obstruction of normal metabolic exchange at the silicone-tissue interface. The incidence of complications causing deterioration of vision or serious symptoms was not found to be high, and navigating vision was well preserved."}
{"id": "PMID:465415", "title": "Efficacy of acycloguanosine against herpetic ulcers in rabbit cornea.", "content": "The effect of a new antiherpetic compound of very low toxicity called acycloguanosine (Wellcome 248U) on herpetic ulcers in rabbit cornea was studied by the Corneal Epithelial Lesion Therapeutic Assay (CELTA). The therapeutic effect of 3% acycloguanosine ointment on dendritic ulcers was equal to that of 0.5% 5-iodo-2'-deoxyuridine (IDU) ointment. No toxic symptoms could be detected by slit-lamp on 4 days' treatment with this concentration of acycloguanosine. Because of its selective action on virus only, its extremely low toxicity in animals, and its availability for systemic administration, acycloguanosine seems to be an ideal antiviral compound for use in the treatment not only of herpetic keratitis but also of other herpetic diseases in man.", "contents": "Efficacy of acycloguanosine against herpetic ulcers in rabbit cornea. The effect of a new antiherpetic compound of very low toxicity called acycloguanosine (Wellcome 248U) on herpetic ulcers in rabbit cornea was studied by the Corneal Epithelial Lesion Therapeutic Assay (CELTA). The therapeutic effect of 3% acycloguanosine ointment on dendritic ulcers was equal to that of 0.5% 5-iodo-2'-deoxyuridine (IDU) ointment. No toxic symptoms could be detected by slit-lamp on 4 days' treatment with this concentration of acycloguanosine. Because of its selective action on virus only, its extremely low toxicity in animals, and its availability for systemic administration, acycloguanosine seems to be an ideal antiviral compound for use in the treatment not only of herpetic keratitis but also of other herpetic diseases in man."}
{"id": "PMID:465416", "title": "Experimental Pseudomonas keratitis in guinea-pigs: therapy of moderately severe infections.", "content": "We have previously shown that antibiotic therapy of experimental Pseudomonas keratitis was more effective in early moderate infections than in late severe infections. The purpose of this study was to determine the relative efficacies of various drugs, routes, and vehicles in the treatment of moderately severe infection. As in the late severe infections, the most consistently effective regimen was an aminoglycoside applied topically in solution. No synergistic or additive effect was observed with a combination of aminoglycoside given topically and a penicillin given intramuscularly. Topical therapy with antibiotic in ointment was less effective than topical therapy with antibiotic in solution.", "contents": "Experimental Pseudomonas keratitis in guinea-pigs: therapy of moderately severe infections. We have previously shown that antibiotic therapy of experimental Pseudomonas keratitis was more effective in early moderate infections than in late severe infections. The purpose of this study was to determine the relative efficacies of various drugs, routes, and vehicles in the treatment of moderately severe infection. As in the late severe infections, the most consistently effective regimen was an aminoglycoside applied topically in solution. No synergistic or additive effect was observed with a combination of aminoglycoside given topically and a penicillin given intramuscularly. Topical therapy with antibiotic in ointment was less effective than topical therapy with antibiotic in solution."}
{"id": "PMID:465417", "title": "Survey of ophthalmic conditions in a Labrador community. I. Refractive errors.", "content": "Of the 745 available members of the population of Nain in Labrador 650 (87%) were screened for refractive errors and ocular disease. Refraction by retinoscopy was done in 553 and axial length measured by an optical method in 514. The results showed that the incidence of low degrees of myopia was higher in Inuit (Eskimos) and those of Mixed Inuit-Caucasian blood in the age groups 10 to 40 than in those over 40. 75% of the myopes came from 20 families in which myopia was present in 2 or more generations. Although there was no significant correlation between the refraction of parents and offspring, there were significant correlations between them for axial length. The axial lenths of the myopic eyes of the Inuit and Mixed populations were significantly longer than emmetropic and hypermetropic eyes. The younger memebers of the population were taller than their parents, and except in female Caucasians axial length showed a significant positive correlation with height. More myopes than emmetropes and hypermetropes achieved grade 8 or more in school. It is suggested that the increased incidence of myopia in the younger age groups might be due to environmental factors interfering with the process of emmetropisation in eyes with a genetic predisposition to myopia by virtue of inheriting a slightlt longer eye. Better nutrition resulting in an increase in stature may also have had some influence.", "contents": "Survey of ophthalmic conditions in a Labrador community. I. Refractive errors. Of the 745 available members of the population of Nain in Labrador 650 (87%) were screened for refractive errors and ocular disease. Refraction by retinoscopy was done in 553 and axial length measured by an optical method in 514. The results showed that the incidence of low degrees of myopia was higher in Inuit (Eskimos) and those of Mixed Inuit-Caucasian blood in the age groups 10 to 40 than in those over 40. 75% of the myopes came from 20 families in which myopia was present in 2 or more generations. Although there was no significant correlation between the refraction of parents and offspring, there were significant correlations between them for axial length. The axial lenths of the myopic eyes of the Inuit and Mixed populations were significantly longer than emmetropic and hypermetropic eyes. The younger memebers of the population were taller than their parents, and except in female Caucasians axial length showed a significant positive correlation with height. More myopes than emmetropes and hypermetropes achieved grade 8 or more in school. It is suggested that the increased incidence of myopia in the younger age groups might be due to environmental factors interfering with the process of emmetropisation in eyes with a genetic predisposition to myopia by virtue of inheriting a slightlt longer eye. Better nutrition resulting in an increase in stature may also have had some influence."}
{"id": "PMID:465419", "title": "Influence of a prolonged period of low-dosage x-rays on the optic and ultrastructural appearances of cataract of the human lens.", "content": "Human lenses extracted for cataract 26 years after long-term exposure to an imperfectly shielded radium source were examined by slit-lamp photography, thin-section light microscopy, and electron microscopy. Anterior epithelial cells were fibroblast-like, and germinal epithelium and vacuolated cortical fibres had accumulated at the equator. A zone of light scatter at the anterior pole corresponded to an area of breakdown of cortical lens fibres, where unusual feathery fibres were orientated perpendicular to the lens surface. Two zones of light scatter separated by a 250-microM clear interval were seen in the posterior cortex. The zone at the posterior pole corresponded to an area of fibre liquefaction and large rounded membrane whorls, while the deeper zone comprised small flattened membrane whorls. The characteristic plaques of swollen abnormal cells described in previous histological studies of x-ray cataract were not present. This and other differences probably reflect the extremely long time course and repeated subliminal doses to which the patient was exposed.", "contents": "Influence of a prolonged period of low-dosage x-rays on the optic and ultrastructural appearances of cataract of the human lens. Human lenses extracted for cataract 26 years after long-term exposure to an imperfectly shielded radium source were examined by slit-lamp photography, thin-section light microscopy, and electron microscopy. Anterior epithelial cells were fibroblast-like, and germinal epithelium and vacuolated cortical fibres had accumulated at the equator. A zone of light scatter at the anterior pole corresponded to an area of breakdown of cortical lens fibres, where unusual feathery fibres were orientated perpendicular to the lens surface. Two zones of light scatter separated by a 250-microM clear interval were seen in the posterior cortex. The zone at the posterior pole corresponded to an area of fibre liquefaction and large rounded membrane whorls, while the deeper zone comprised small flattened membrane whorls. The characteristic plaques of swollen abnormal cells described in previous histological studies of x-ray cataract were not present. This and other differences probably reflect the extremely long time course and repeated subliminal doses to which the patient was exposed."}
{"id": "PMID:465420", "title": "Cataract extraction complicated by capsular glaucoma.", "content": "Synechiae involving the iris pigment epithelium and the peripheral anterior lens capsule occur in capsular glaucoma. During cataract extraction they may bring about traction on the capsule threatening its rupture. The ultrastructural basis of these adhesions is a bonding of pigment cells to capsular surface by pseudoexfoliative material.", "contents": "Cataract extraction complicated by capsular glaucoma. Synechiae involving the iris pigment epithelium and the peripheral anterior lens capsule occur in capsular glaucoma. During cataract extraction they may bring about traction on the capsule threatening its rupture. The ultrastructural basis of these adhesions is a bonding of pigment cells to capsular surface by pseudoexfoliative material."}
{"id": "PMID:465421", "title": "Trial of a laminated material to reduce glare in cataract.", "content": "A recently-introduced laminated material which accepts light only over a small angle on either side of the normal was compared to standard Polaroid sunglasses. A preliminary trial failed to show any superiority of the new material in reducing glare for patients with moderate cataract.", "contents": "Trial of a laminated material to reduce glare in cataract. A recently-introduced laminated material which accepts light only over a small angle on either side of the normal was compared to standard Polaroid sunglasses. A preliminary trial failed to show any superiority of the new material in reducing glare for patients with moderate cataract."}
{"id": "PMID:465422", "title": "'Silicone rubber' lenses in aphakia.", "content": "Tesicon, one of the commercially available 'silicone rubber' lenses, was used in the correction of aphakic patients. In 74% of cases the lenses were considered successful for a daily wear regimen by the patient. Furthermore, a small number of patients could wear this lens without interruption for 3 to 6 days at a time. Despite this good acceptance by patients, corneal problems (mainly staining) and lens problems (dry surfaces) were frequently encountered.", "contents": "'Silicone rubber' lenses in aphakia. Tesicon, one of the commercially available 'silicone rubber' lenses, was used in the correction of aphakic patients. In 74% of cases the lenses were considered successful for a daily wear regimen by the patient. Furthermore, a small number of patients could wear this lens without interruption for 3 to 6 days at a time. Despite this good acceptance by patients, corneal problems (mainly staining) and lens problems (dry surfaces) were frequently encountered."}
{"id": "PMID:465423", "title": "Endothelial bedewing of the cornea in association with contact lens wear.", "content": "Endothelial bedewing is described as either a cluster of oedematous droplets or a constellation of leucocytes deposited on the surface of the corneal endothelium. The use of marginal retroilumination in biomicroscopy is described for the detection of endothelial bedewing. Some of the clinical characteristics of endothelial bedewing are outlined, and its occurrence in patients who are chronically endothelial bedewing may be indicative of a limited anterior uveal response associated with contact lens wear.", "contents": "Endothelial bedewing of the cornea in association with contact lens wear. Endothelial bedewing is described as either a cluster of oedematous droplets or a constellation of leucocytes deposited on the surface of the corneal endothelium. The use of marginal retroilumination in biomicroscopy is described for the detection of endothelial bedewing. Some of the clinical characteristics of endothelial bedewing are outlined, and its occurrence in patients who are chronically endothelial bedewing may be indicative of a limited anterior uveal response associated with contact lens wear."}
{"id": "PMID:465424", "title": "An improved diagnostic contact lens.", "content": "Modification of a standard Goldmann goniolens by reducing the curvature of the contact surface to 8.5 mm radius of curvature (from the standard curvature of 7.4 mm) enabled gonioscopy to be carried out without the nuisance of air bubbles.", "contents": "An improved diagnostic contact lens. Modification of a standard Goldmann goniolens by reducing the curvature of the contact surface to 8.5 mm radius of curvature (from the standard curvature of 7.4 mm) enabled gonioscopy to be carried out without the nuisance of air bubbles."}
{"id": "PMID:465425", "title": "Visual loss and foveal lesions in Usher's syndrome.", "content": "Forty-eight patients with Usher's syndrome were assess for the degree of visual loss, extent of lens opacities, and presence of foveal lesions. Twenty-one patients (44%) showed either atrophic or cystic-appearing bilateral foveal changes. Despite having foveal lesions and variable degrees of lens opacities most patients maintained central visual acuity of 20/60 or better until their middle 30s.", "contents": "Visual loss and foveal lesions in Usher's syndrome. Forty-eight patients with Usher's syndrome were assess for the degree of visual loss, extent of lens opacities, and presence of foveal lesions. Twenty-one patients (44%) showed either atrophic or cystic-appearing bilateral foveal changes. Despite having foveal lesions and variable degrees of lens opacities most patients maintained central visual acuity of 20/60 or better until their middle 30s."}
{"id": "PMID:465426", "title": "Excessive permeability in diabetic maculopathy.", "content": "Four cases of diabetic maculopathy with excessive permeability are described. Fluorescence angiography is distinctive, showing profuse early leakage from the entire capillary bed of the posterior pole. Careful studies have failed to reveal any cause for this excessive permeability response at the macula or any constantly associated medical abnormality. The prognosis for visual acuity is poor, and photocoagulation has only rarely been successful in maintaining or improving vision in these patients.", "contents": "Excessive permeability in diabetic maculopathy. Four cases of diabetic maculopathy with excessive permeability are described. Fluorescence angiography is distinctive, showing profuse early leakage from the entire capillary bed of the posterior pole. Careful studies have failed to reveal any cause for this excessive permeability response at the macula or any constantly associated medical abnormality. The prognosis for visual acuity is poor, and photocoagulation has only rarely been successful in maintaining or improving vision in these patients."}
{"id": "PMID:465427", "title": "Macular pigment deposits in rhegmatogenous retinal detachment.", "content": "This is a study of 20 cases of macular migration after operation for retinal detachment. The frequency of pigment deposits on the macula among the total number of operated detachments was 6.4%. The evolution of pigment migration depened on the form and type of the deposits. In some cases the pigment diminished or disappeared. In 6 of the 20 eyes with pigment migration puckering of the macula occurred 4 months or more after surgery. The improvement in visual acuity 6 months and more after operation was relatively less in the group with pigment deposits than in the group without pigment. In the former group static perimetry showed defects corresponding to the pigment migration.", "contents": "Macular pigment deposits in rhegmatogenous retinal detachment. This is a study of 20 cases of macular migration after operation for retinal detachment. The frequency of pigment deposits on the macula among the total number of operated detachments was 6.4%. The evolution of pigment migration depened on the form and type of the deposits. In some cases the pigment diminished or disappeared. In 6 of the 20 eyes with pigment migration puckering of the macula occurred 4 months or more after surgery. The improvement in visual acuity 6 months and more after operation was relatively less in the group with pigment deposits than in the group without pigment. In the former group static perimetry showed defects corresponding to the pigment migration."}
{"id": "PMID:465428", "title": "The fishmouth phenomenon in retinal detachment: old concepts revisited.", "content": "The 'fishmouth phenomenon' seen in some previously treated retinal detachments is associated with large horeshoe tears and scleral buckling techniques. A method of treating patients with this complication is described in which scleral buckling with implant or explant is not used. The technique utilises intravitreal air tamponade with cryopexy and gives good results. The incidence of patients developing the fishmouth phenomenon as a complication of parimary simple retinal detachment surgery is much lower when an intravitreal air technique is used than the expected incidence following a primary scleral buckling operation.", "contents": "The fishmouth phenomenon in retinal detachment: old concepts revisited. The 'fishmouth phenomenon' seen in some previously treated retinal detachments is associated with large horeshoe tears and scleral buckling techniques. A method of treating patients with this complication is described in which scleral buckling with implant or explant is not used. The technique utilises intravitreal air tamponade with cryopexy and gives good results. The incidence of patients developing the fishmouth phenomenon as a complication of parimary simple retinal detachment surgery is much lower when an intravitreal air technique is used than the expected incidence following a primary scleral buckling operation."}
{"id": "PMID:465429", "title": "Anhidrotic ectodermal dysplasia with lacrimal anomalies.", "content": "Ectodermal dysplasia and its related disorders may present with ocular symptoms due to involvement of the ectodermal components of the eye. This paper reports a case of congenital anhidrotic ectodermal dysplasia presenting to the ophthalmologist with epiphora due to lacrimal atresia. The embryonic origin of the lacrimal system suggests that lacrimal anomalies in association with ectodermal disorders are commoner than reports indicate. Their management consists in accurate evaluation of the anomaly followed by surgery.", "contents": "Anhidrotic ectodermal dysplasia with lacrimal anomalies. Ectodermal dysplasia and its related disorders may present with ocular symptoms due to involvement of the ectodermal components of the eye. This paper reports a case of congenital anhidrotic ectodermal dysplasia presenting to the ophthalmologist with epiphora due to lacrimal atresia. The embryonic origin of the lacrimal system suggests that lacrimal anomalies in association with ectodermal disorders are commoner than reports indicate. Their management consists in accurate evaluation of the anomaly followed by surgery."}
{"id": "PMID:465430", "title": "Inequality of the direct and consensual light reflexes in normal subjects.", "content": "Anisocoria in darkness and during reflex responses to unilateral light stimulation was studied in 150 normal subjects with television pupillometry. It was commonly found that the direct light reaction of the stimulation eye exceeded the consensual reaction of the other eye. This light-induced anisocoria, termed 'contraction anisocoria', had a mean value of 0.075 mm or 6.1 % of light reflex amplitude. The measurement showed a high degree of repeatability in 20 subjects who were tested on two occasions a year apart. It occurred in the presence and absence of prior dark adaptation and increased proportionally with reflex amplitude as the intensity of the stimulating light was raised. IT IS CONCLUDED THAT, CONTRARY TO PREVIOUS OPINION, A SMALL DEGREE OF CONTRACTION ANISOCORIA IS NORMAL.", "contents": "Inequality of the direct and consensual light reflexes in normal subjects. Anisocoria in darkness and during reflex responses to unilateral light stimulation was studied in 150 normal subjects with television pupillometry. It was commonly found that the direct light reaction of the stimulation eye exceeded the consensual reaction of the other eye. This light-induced anisocoria, termed 'contraction anisocoria', had a mean value of 0.075 mm or 6.1 % of light reflex amplitude. The measurement showed a high degree of repeatability in 20 subjects who were tested on two occasions a year apart. It occurred in the presence and absence of prior dark adaptation and increased proportionally with reflex amplitude as the intensity of the stimulating light was raised. IT IS CONCLUDED THAT, CONTRARY TO PREVIOUS OPINION, A SMALL DEGREE OF CONTRACTION ANISOCORIA IS NORMAL."}
{"id": "PMID:465431", "title": "Leucocyte ascorbic acid and the leucocyte count.", "content": "1. During the course of a nutritional survey on an Antarctic base, leucocyte ascorbic acid estimations (Denson & Bowers, 1961) were found to be significantly affected (P less than 0.0001) by the leucocyte count measured during analysis. 2. The effect was significantly less (P less than 0.05) when leucocyte levels were at or near saturation than when subsaturated. 3. This suggests that leucocytes share available ascorbic acid, especially in subsaturated subjects, and that assessment of nutritional status from the leucocyte ascorbic acid level should take account of the leucocyte count and the plasma ascorbic acid titre.", "contents": "Leucocyte ascorbic acid and the leucocyte count. 1. During the course of a nutritional survey on an Antarctic base, leucocyte ascorbic acid estimations (Denson & Bowers, 1961) were found to be significantly affected (P less than 0.0001) by the leucocyte count measured during analysis. 2. The effect was significantly less (P less than 0.05) when leucocyte levels were at or near saturation than when subsaturated. 3. This suggests that leucocytes share available ascorbic acid, especially in subsaturated subjects, and that assessment of nutritional status from the leucocyte ascorbic acid level should take account of the leucocyte count and the plasma ascorbic acid titre."}
{"id": "PMID:465432", "title": "A preliminary simulated iron fortification trial in South Indian preschool children.", "content": "1. The effect of fortification of food with iron to provide 10 mg elemental Fe/child per d was studied in preschool children maintained on a cereal diet, over a 5-month period. 2. The absorption of 5 mg Fe as ferrous sulphate mixed in one meal was 3.3% of the test dose and when 3.3 mg was given with each of three meals over a 2 d period the corresponding value was 4.8%. 3. The mean absorption of a test dose of ferrous ascorbate studied in twenty-four children midway through the trial was 42%. 4. The only beneficial effect of Fe fortification in this time-period in the experimental group was the prevention of the decrease in packed cell volume which occurred in the control groups.", "contents": "A preliminary simulated iron fortification trial in South Indian preschool children. 1. The effect of fortification of food with iron to provide 10 mg elemental Fe/child per d was studied in preschool children maintained on a cereal diet, over a 5-month period. 2. The absorption of 5 mg Fe as ferrous sulphate mixed in one meal was 3.3% of the test dose and when 3.3 mg was given with each of three meals over a 2 d period the corresponding value was 4.8%. 3. The mean absorption of a test dose of ferrous ascorbate studied in twenty-four children midway through the trial was 42%. 4. The only beneficial effect of Fe fortification in this time-period in the experimental group was the prevention of the decrease in packed cell volume which occurred in the control groups."}
{"id": "PMID:465433", "title": "Comparison of carbohydrate utilization in man using indirect calorimetry and mass spectrometry after an oral load of 100 g naturally-labelled [13C]glucose.", "content": "1. Carbohydrate (CHO) oxidation was measured simultaneously in a group of five normal subjects after an oral load of 100 g naturally-labelled [13C]glucose, using indirect calorimetry and mass spectrometry. 2. CHO utilization, calculated from the results of indirect calorimetry, increased 30 min after the glucose load to reach a peak at 90 min. It then decreased to reach basal values at 380 min. Cumulative total CHO oxidation at 480 min was 83 +/- 8 g, and CHO oxidized above basal levels, 37 +/- 3 g. 3. Enrichment of expired carbon dioxide with 13C began at 60 min and maximum values were observed at 270 min. At 480 min, cumulative CHO oxidation measured by use of [13C]glucose was 29 g. The difference from calorimetric values can be attributed in part to the slow isotopic dilution in the glucose and bicarbonate pools. 4. Thus, approximately 30% of the glucose load was oxidized during the 8 h after its ingestion and this accounts for a significant part of the increased CHO oxidation (37 g), as measured by indirect calorimetry.", "contents": "Comparison of carbohydrate utilization in man using indirect calorimetry and mass spectrometry after an oral load of 100 g naturally-labelled [13C]glucose. 1. Carbohydrate (CHO) oxidation was measured simultaneously in a group of five normal subjects after an oral load of 100 g naturally-labelled [13C]glucose, using indirect calorimetry and mass spectrometry. 2. CHO utilization, calculated from the results of indirect calorimetry, increased 30 min after the glucose load to reach a peak at 90 min. It then decreased to reach basal values at 380 min. Cumulative total CHO oxidation at 480 min was 83 +/- 8 g, and CHO oxidized above basal levels, 37 +/- 3 g. 3. Enrichment of expired carbon dioxide with 13C began at 60 min and maximum values were observed at 270 min. At 480 min, cumulative CHO oxidation measured by use of [13C]glucose was 29 g. The difference from calorimetric values can be attributed in part to the slow isotopic dilution in the glucose and bicarbonate pools. 4. Thus, approximately 30% of the glucose load was oxidized during the 8 h after its ingestion and this accounts for a significant part of the increased CHO oxidation (37 g), as measured by indirect calorimetry."}
{"id": "PMID:465434", "title": "Relationship between biochemical and clinical indices of B-vitamin deficiency. A study in rural school boys.", "content": "1. A study amongst schoolboys in villages around Hyderabad, India, showed that almost all the boys had riboflavin deficiency, 61% had pyridoxine deficiency, and 9.4% had thiamin deficiency as judged by enzymic tests. 2. The prevalence of angular stomatitis was 41.3% and that of glossitis was 18.2%. Biochemical deficiency of riboflavin and pyridoxine was marginally higher in children with angular stomatitis with or without associated glossitis, than in children without oral lesions. 3. Treatment with B-complex vitamins (containing 4 mg riboflavin and 10 mg pyridoxine) daily for 1 month produced significant reduction in the prevalence of glossitis but had no effect on angular stomatitis. The latter responded to topical application of gentian violet. 4. Small but significant changes in erythrocyte enzymes occurred over the period of 1 month even without vitamin supplements. 5. Results suggest that while glossitis is a relatively early manifestation of riboflavin or pyridoxine deficiency or both, angular stomatitis has a more complex aetiology perphaps associated with infection.", "contents": "Relationship between biochemical and clinical indices of B-vitamin deficiency. A study in rural school boys. 1. A study amongst schoolboys in villages around Hyderabad, India, showed that almost all the boys had riboflavin deficiency, 61% had pyridoxine deficiency, and 9.4% had thiamin deficiency as judged by enzymic tests. 2. The prevalence of angular stomatitis was 41.3% and that of glossitis was 18.2%. Biochemical deficiency of riboflavin and pyridoxine was marginally higher in children with angular stomatitis with or without associated glossitis, than in children without oral lesions. 3. Treatment with B-complex vitamins (containing 4 mg riboflavin and 10 mg pyridoxine) daily for 1 month produced significant reduction in the prevalence of glossitis but had no effect on angular stomatitis. The latter responded to topical application of gentian violet. 4. Small but significant changes in erythrocyte enzymes occurred over the period of 1 month even without vitamin supplements. 5. Results suggest that while glossitis is a relatively early manifestation of riboflavin or pyridoxine deficiency or both, angular stomatitis has a more complex aetiology perphaps associated with infection."}
{"id": "PMID:465435", "title": "Effects of diet, energy restriction and diabetes on hexose transport in the rat.", "content": "1. Effects of dietary composition, energy restriction, and diabetes on hexose absorption were examined by feeding male rats isoenergetic, semi-synthetic diets of differing carbohydrate and protein content. Diets were carbohydrate, (g/kg): 890 sucrose; carbohydrate-protien, 500 sucrose, 390 casein; or protein, 890 casein. An additional group was fed on commercial rat chow ad lib. 2. Hexose (3-O-methyl-D-glucose) absorption was measured by luminal perfusion of the entire small intestine in situ. Absorption by the total small intestine, i.e. absorption per rat, and absorption per g dry weight of mucosa (specific absorption) were calculated. 3. When semi-synthetic diets were fed at 210 kJ/d to normal animals absorption depended on composition of diets: carbohydrate enhanced or protein suppressed hexose absorption. Dietary carbohydrate as glucose, dextrimaltose or starch gave the same hexose absorption response as sucrose. 4. When diets of normal rats were restricted to 118 kJ/d, specific absorption was independent of dietary composition and was increased for all dietary groups to the level of the group fed on the carbohydrate diet at 210 kJ/d. 5. When diabetic rats were given 210 kJ/d, hexose specific absorption was the same for all diabetic groups independent of dietary composition and was equal to that of controls given carbohydrate, but greater than that of protein-fed controls. 6. Thus, when two of the three stimuli (i.e. carbohydrate diet plus energy restriction or diabetes) were combined, the effect was not additive, and the response of hexose specific absorption to diabetes and energy restriction was the same: absorption was independent of dietary composition and was stimulated relative to controls fed on diets containing protein. 7. The pattern of response of total small intestinal hexose absorption to the stimuli of dietary composition, energy restriction and diabetes was similar to that of specific absorption. 8. Compared with groups given semi-synthetic diets, rats eating commercial rat chow ad lib. (approximately 286 kJ/d) showed increased mucosal mass and decreased specific absorption, but total absorption was similar to that of the carbohydrate and carbohydrate-protein-fed groups. 9. In a separate study in control rats, specific and total intestinal absorption of L-leucine did not respond to dietary composition, i.e. level of protein fed.", "contents": "Effects of diet, energy restriction and diabetes on hexose transport in the rat. 1. Effects of dietary composition, energy restriction, and diabetes on hexose absorption were examined by feeding male rats isoenergetic, semi-synthetic diets of differing carbohydrate and protein content. Diets were carbohydrate, (g/kg): 890 sucrose; carbohydrate-protien, 500 sucrose, 390 casein; or protein, 890 casein. An additional group was fed on commercial rat chow ad lib. 2. Hexose (3-O-methyl-D-glucose) absorption was measured by luminal perfusion of the entire small intestine in situ. Absorption by the total small intestine, i.e. absorption per rat, and absorption per g dry weight of mucosa (specific absorption) were calculated. 3. When semi-synthetic diets were fed at 210 kJ/d to normal animals absorption depended on composition of diets: carbohydrate enhanced or protein suppressed hexose absorption. Dietary carbohydrate as glucose, dextrimaltose or starch gave the same hexose absorption response as sucrose. 4. When diets of normal rats were restricted to 118 kJ/d, specific absorption was independent of dietary composition and was increased for all dietary groups to the level of the group fed on the carbohydrate diet at 210 kJ/d. 5. When diabetic rats were given 210 kJ/d, hexose specific absorption was the same for all diabetic groups independent of dietary composition and was equal to that of controls given carbohydrate, but greater than that of protein-fed controls. 6. Thus, when two of the three stimuli (i.e. carbohydrate diet plus energy restriction or diabetes) were combined, the effect was not additive, and the response of hexose specific absorption to diabetes and energy restriction was the same: absorption was independent of dietary composition and was stimulated relative to controls fed on diets containing protein. 7. The pattern of response of total small intestinal hexose absorption to the stimuli of dietary composition, energy restriction and diabetes was similar to that of specific absorption. 8. Compared with groups given semi-synthetic diets, rats eating commercial rat chow ad lib. (approximately 286 kJ/d) showed increased mucosal mass and decreased specific absorption, but total absorption was similar to that of the carbohydrate and carbohydrate-protein-fed groups. 9. In a separate study in control rats, specific and total intestinal absorption of L-leucine did not respond to dietary composition, i.e. level of protein fed."}
{"id": "PMID:465436", "title": "The effect of wheat bran and pectin on paracetamol absorption in the rat.", "content": "1. The absorption of an oral dose of paracetamol was measured in rats given either a basal fibre-free diet, or the basal diet with either pectin or bran added. 2. Urinary excretion of the oral dose was significantly greater during the first 8 h in the pectin-fed rats compared with those on basal diet, though cumulative excretion after 72 h was the same. 3. Free paracetamol levels in the plasma were significantly higher in the pectin-fed rats compared with those on basal diet at 30, 60, 90 and 120 min after the oral dose. 4. The plasma half-life of intravenously-injected paracetamol was shorter in the pectin-fed rats than in those on basal diet alone, but the antipyrine half-lives were not significantly different. 5. Pectin feeding had no effect on either the apparent volume of distribution of paracetamol and antipyrine, or on the rate of gastric empyting. 6. Perfusion of the whole length of the small bowel showed a significantly greater capacity for paracetamol absorption in the pectin-fed rats. 7. Bran had no effect on paracetamol absorption. 8. It was concluded that dietary fibre intake affects drug absorption and that the effect varies with the type of fibre. Unexpectedly pectin accelerates rather than retards absorption of paracetamol, though the mechansim of this effect is unknown.", "contents": "The effect of wheat bran and pectin on paracetamol absorption in the rat. 1. The absorption of an oral dose of paracetamol was measured in rats given either a basal fibre-free diet, or the basal diet with either pectin or bran added. 2. Urinary excretion of the oral dose was significantly greater during the first 8 h in the pectin-fed rats compared with those on basal diet, though cumulative excretion after 72 h was the same. 3. Free paracetamol levels in the plasma were significantly higher in the pectin-fed rats compared with those on basal diet at 30, 60, 90 and 120 min after the oral dose. 4. The plasma half-life of intravenously-injected paracetamol was shorter in the pectin-fed rats than in those on basal diet alone, but the antipyrine half-lives were not significantly different. 5. Pectin feeding had no effect on either the apparent volume of distribution of paracetamol and antipyrine, or on the rate of gastric empyting. 6. Perfusion of the whole length of the small bowel showed a significantly greater capacity for paracetamol absorption in the pectin-fed rats. 7. Bran had no effect on paracetamol absorption. 8. It was concluded that dietary fibre intake affects drug absorption and that the effect varies with the type of fibre. Unexpectedly pectin accelerates rather than retards absorption of paracetamol, though the mechansim of this effect is unknown."}
{"id": "PMID:465437", "title": "The effect of dietary fats on the composition of the liver endoplasmic reticulum and oxidative drug metabolsim.", "content": "1. The dependence of the rate of oxidative demethylation in the liver endoplasmic reticulum on the fatty acid composition of the endoplasmic reticulum has been studied by varying the lipid content of the diet. 2. The rate of oxidative demethylation was markedly dependent on the percentage of linoleic acid (18:2) incorporated into the membrane. Feeding diets containing (g/kg) 100 coconut oil, 100 lard or 100 maize oil caused respectively the incorporation of 7.6, 10.3 and 25.1% linoleic acid (18:2) and a demethylation rate 3.26, 3.15 and 5.03 nmol formaldehyde/min per mg protein. Feeding 100 g herring oil/kg diet caused incorporation of only 5.1% C18:2 but also 27.2% omega 3 unsaturated fatty acids, including 8.7% eicosapentaenoic acid (20:5) and 17.0% docosahexaenoic acid (22.6) and caused a very high rate of oxidative demethylation (6.53 nmol formaldehyde/min per mg protein). 3. Destruction of the polyunsaturated fatty acids in herring oil by irradiation with 400 krad caused incorporation of a smaller quantity of 3 omega unsaturated acids into the endoplasmic reticulum and decreased the rate of oxidative demethylation (4.83 nmol formaldehyde/min per mg protein). 4. The inductive effects of phenobarbitone on oxidative demethylation were partially dependent on changes in the fatty acid composition of the endoplasmic reticulum. Phenobarbitone (100 mg/kg) increased the percentage of C18:2 from 25.1 to 29.4% in rats given a maize-oil diet, increased the percentage of C20:5 from 8.7 to 10.3% in rats given a herring-oil diet and decreased the percentage of arachidonic acid (20:4) and C22.6 in rats given a lard, maize-oil, herring-oil or irradiated-herring-oil diet. 5. Intraperitoneal alpha-tocopherol (50 mg/kg) increased the percentage of C20:4 from 11.1 to 13.1% in rats given a lard diet and from 5.9 to 7.3% in rats given a herring-oil diet. 6. It is concluded that dietary C18:2 is an important factor in the regulation of the rate of oxidative demethylation in the liver endoplasmic reticulum but this may be replaced effectively by dietary C20:5 omega 3 and C22:6 omega 3 acids. Oxidative demethylation is regulated by changes in the fatty acid composition of the membranes of the liver endoplasmic reticulum.", "contents": "The effect of dietary fats on the composition of the liver endoplasmic reticulum and oxidative drug metabolsim. 1. The dependence of the rate of oxidative demethylation in the liver endoplasmic reticulum on the fatty acid composition of the endoplasmic reticulum has been studied by varying the lipid content of the diet. 2. The rate of oxidative demethylation was markedly dependent on the percentage of linoleic acid (18:2) incorporated into the membrane. Feeding diets containing (g/kg) 100 coconut oil, 100 lard or 100 maize oil caused respectively the incorporation of 7.6, 10.3 and 25.1% linoleic acid (18:2) and a demethylation rate 3.26, 3.15 and 5.03 nmol formaldehyde/min per mg protein. Feeding 100 g herring oil/kg diet caused incorporation of only 5.1% C18:2 but also 27.2% omega 3 unsaturated fatty acids, including 8.7% eicosapentaenoic acid (20:5) and 17.0% docosahexaenoic acid (22.6) and caused a very high rate of oxidative demethylation (6.53 nmol formaldehyde/min per mg protein). 3. Destruction of the polyunsaturated fatty acids in herring oil by irradiation with 400 krad caused incorporation of a smaller quantity of 3 omega unsaturated acids into the endoplasmic reticulum and decreased the rate of oxidative demethylation (4.83 nmol formaldehyde/min per mg protein). 4. The inductive effects of phenobarbitone on oxidative demethylation were partially dependent on changes in the fatty acid composition of the endoplasmic reticulum. Phenobarbitone (100 mg/kg) increased the percentage of C18:2 from 25.1 to 29.4% in rats given a maize-oil diet, increased the percentage of C20:5 from 8.7 to 10.3% in rats given a herring-oil diet and decreased the percentage of arachidonic acid (20:4) and C22.6 in rats given a lard, maize-oil, herring-oil or irradiated-herring-oil diet. 5. Intraperitoneal alpha-tocopherol (50 mg/kg) increased the percentage of C20:4 from 11.1 to 13.1% in rats given a lard diet and from 5.9 to 7.3% in rats given a herring-oil diet. 6. It is concluded that dietary C18:2 is an important factor in the regulation of the rate of oxidative demethylation in the liver endoplasmic reticulum but this may be replaced effectively by dietary C20:5 omega 3 and C22:6 omega 3 acids. Oxidative demethylation is regulated by changes in the fatty acid composition of the membranes of the liver endoplasmic reticulum."}
{"id": "PMID:465438", "title": "Trace nutrients. 3 Magnesium, copper, zinc, vitamin B6, vitamin B12 and folic acid in the British household food supply.", "content": "1. Intakes of magnesium, copper, zinc, vitamin B6, vitamin B12 and folic acid in Britain were calculated by applying the values selected for the 4th edition of McCance and Widdowson's The Composition of Foods (Paul & Southgate, 1978) to the amounts of food recorded in the National Food Survey made during 1976 (Ministry of Agriculture, Fisheries and Food, 1977). 2. National average intakes were (/person per d): Mg 249 mg, Cu 1.51 mg, Zn 9.1 mg, vitamin B6 1.36 mg, vitamin B12 6.6 micrograms free folic acid 105 microgram and total folic acid 190 micrograms. Corresponding intakes (/person) in families with four or more children were 10-20% lower. 3. A comparison of intakes with those recommended in Canada (Department of National Health and Welfare, 1976) and the USA (National Research Council, 1974) or by WHO (1973) indicated that for folic acid average values were particularly low, and only for vitamin B12 were the recommendations significantly exceeded. 4. Contributions from alcoholic drinks and confectionery were also calculated and found, on average, to be significant for cu, vitamin B12 and folic acid. 5. The losses of B-vitamins which might occur on cooking are discussed.", "contents": "Trace nutrients. 3 Magnesium, copper, zinc, vitamin B6, vitamin B12 and folic acid in the British household food supply. 1. Intakes of magnesium, copper, zinc, vitamin B6, vitamin B12 and folic acid in Britain were calculated by applying the values selected for the 4th edition of McCance and Widdowson's The Composition of Foods (Paul & Southgate, 1978) to the amounts of food recorded in the National Food Survey made during 1976 (Ministry of Agriculture, Fisheries and Food, 1977). 2. National average intakes were (/person per d): Mg 249 mg, Cu 1.51 mg, Zn 9.1 mg, vitamin B6 1.36 mg, vitamin B12 6.6 micrograms free folic acid 105 microgram and total folic acid 190 micrograms. Corresponding intakes (/person) in families with four or more children were 10-20% lower. 3. A comparison of intakes with those recommended in Canada (Department of National Health and Welfare, 1976) and the USA (National Research Council, 1974) or by WHO (1973) indicated that for folic acid average values were particularly low, and only for vitamin B12 were the recommendations significantly exceeded. 4. Contributions from alcoholic drinks and confectionery were also calculated and found, on average, to be significant for cu, vitamin B12 and folic acid. 5. The losses of B-vitamins which might occur on cooking are discussed."}
{"id": "PMID:465439", "title": "The effects of a supplement of dietary fibre on faecal excretion by human subjects.", "content": "1. Four human subjects on strictly controlled diets were given a fibre supplement, 25 g Ispaghula husk (Isogel)/d, for 3 weeks. 2. Replicate diets and faeces were collected during two 5 d balance periods. The first period served as control for the second which occurred after the supplement had been fed for 2 weeks. 3. Diets and faeces were analysed for total solids, gross energy, total nitrogen, fat, available and unavailable carbohydrates. 4. Three of the four subjects showed a considerable increase in faecal bulk; total faecal weight was more than doubled in two subjects. In two subjects the increase was brought about mainly by extra fibre in the faeces accompanied by a higher proportion of faecal water whereas in one subject it was also an increased excretion of nutrients. One subject showed very little change in faecal bulk. 5. High values for the apparent digestibility of fibre were found during the control period; from 0.70 to 0.80. There was more variation during the experimental period when apparent digestibility of total fibre ranged from 0.47 to 0.82. Values arrived at for the minimum apparent digestibility of the fibre supplement were generally high; one subject appeared to digest Isogel completely. Isogel was degraded preferentially to the food-derived fibre; in particular, cellulose in the faeces was increased during the experimental period. 6. Only one subject showed distinct decreases in the apparent digestibility of energy, N and fat in the diet. The results do not therefore agree with the generally held view that increased fibre in the diet decreases the apparent digestibility of the other nutrients.", "contents": "The effects of a supplement of dietary fibre on faecal excretion by human subjects. 1. Four human subjects on strictly controlled diets were given a fibre supplement, 25 g Ispaghula husk (Isogel)/d, for 3 weeks. 2. Replicate diets and faeces were collected during two 5 d balance periods. The first period served as control for the second which occurred after the supplement had been fed for 2 weeks. 3. Diets and faeces were analysed for total solids, gross energy, total nitrogen, fat, available and unavailable carbohydrates. 4. Three of the four subjects showed a considerable increase in faecal bulk; total faecal weight was more than doubled in two subjects. In two subjects the increase was brought about mainly by extra fibre in the faeces accompanied by a higher proportion of faecal water whereas in one subject it was also an increased excretion of nutrients. One subject showed very little change in faecal bulk. 5. High values for the apparent digestibility of fibre were found during the control period; from 0.70 to 0.80. There was more variation during the experimental period when apparent digestibility of total fibre ranged from 0.47 to 0.82. Values arrived at for the minimum apparent digestibility of the fibre supplement were generally high; one subject appeared to digest Isogel completely. Isogel was degraded preferentially to the food-derived fibre; in particular, cellulose in the faeces was increased during the experimental period. 6. Only one subject showed distinct decreases in the apparent digestibility of energy, N and fat in the diet. The results do not therefore agree with the generally held view that increased fibre in the diet decreases the apparent digestibility of the other nutrients."}
{"id": "PMID:465440", "title": "Guar gum and reduction of post-prandial glycaemia: effect of incorporation into solid food, liquid food, and both.", "content": "1. The influence of the dose and the form in which guar gum was given on the degree of \"flattening\" of blood glucose curves was studied in five subjects using meals of bread and soup containing 5 or 10 g guar gum. 2. When 5 g guar gum was added to bread the peak increase of blood glucose was reduced by 41% (P less than 0.002), with 5 g guar in soup, the reduction was 54% (P less than 0.001) while a reduction of 68% (P less than 0.001) was seen with 10 g guar gum (5 g in bread and 5 g in soup). The corresponding reduction in insulin peak increases were 37% (P less than 0.002), 50% (P less than 0.001) and 65% (P less than 0.001) respectively. 3. The difference between the two 5 g doses was significant with respect to the reduction of the peak increases in blood glucose and serum insulin; however the difference between the 5 g dose in bread and the 10 g dose was significantly different (P less than 0.02 for glucose, P less than 0.01 for insulin). 4. The results indicate that as little as 5 g guar gum may reduce the glycaemia following a 45 g carbohydrate meal, but perhaps due to earlier and more complete mixing, guar gum is most effective when added to the liquid phase of the meal.", "contents": "Guar gum and reduction of post-prandial glycaemia: effect of incorporation into solid food, liquid food, and both. 1. The influence of the dose and the form in which guar gum was given on the degree of \"flattening\" of blood glucose curves was studied in five subjects using meals of bread and soup containing 5 or 10 g guar gum. 2. When 5 g guar gum was added to bread the peak increase of blood glucose was reduced by 41% (P less than 0.002), with 5 g guar in soup, the reduction was 54% (P less than 0.001) while a reduction of 68% (P less than 0.001) was seen with 10 g guar gum (5 g in bread and 5 g in soup). The corresponding reduction in insulin peak increases were 37% (P less than 0.002), 50% (P less than 0.001) and 65% (P less than 0.001) respectively. 3. The difference between the two 5 g doses was significant with respect to the reduction of the peak increases in blood glucose and serum insulin; however the difference between the 5 g dose in bread and the 10 g dose was significantly different (P less than 0.02 for glucose, P less than 0.01 for insulin). 4. The results indicate that as little as 5 g guar gum may reduce the glycaemia following a 45 g carbohydrate meal, but perhaps due to earlier and more complete mixing, guar gum is most effective when added to the liquid phase of the meal."}
{"id": "PMID:465441", "title": "The contribution of protozoa to the protein entering the duodenum of sheep.", "content": "1. Four sheep, each fitted with a rumen fistula and a re-entrant cannula at the proximal duodenum were fed a semi-purified diet containing urea as the only nitrogen source. The quantities of total protozoal amino acid-N (TPAN) present in the rumen and entering the duodenum were determined when the mean rumen dilution rate (D) was low (0.034/h) and when D was increased to 0.078/h by the intraruminal infusion of artificial saliva. 2. Increasing the dilution rate had no significant effect upon the proportions of TPAN present in the total microbial amino-acid-N (TMAN) of the rumen fluid and duodenal digesta. With both dilution rates the mean proportion of TPAN in the duodenal TMAN (0.24) was markedly less than the equivalent proportion (0.45) found in the rumen fluid. 3. The daily flow of TPAN, as measured at the duodenal cannula at both dilution rates were equivalent to only 41% of the flow of TPAN as predicted from measurements of rumen outflow, indicating that a substantial proportion of rumen protozoal protein was retained within the rumen.", "contents": "The contribution of protozoa to the protein entering the duodenum of sheep. 1. Four sheep, each fitted with a rumen fistula and a re-entrant cannula at the proximal duodenum were fed a semi-purified diet containing urea as the only nitrogen source. The quantities of total protozoal amino acid-N (TPAN) present in the rumen and entering the duodenum were determined when the mean rumen dilution rate (D) was low (0.034/h) and when D was increased to 0.078/h by the intraruminal infusion of artificial saliva. 2. Increasing the dilution rate had no significant effect upon the proportions of TPAN present in the total microbial amino-acid-N (TMAN) of the rumen fluid and duodenal digesta. With both dilution rates the mean proportion of TPAN in the duodenal TMAN (0.24) was markedly less than the equivalent proportion (0.45) found in the rumen fluid. 3. The daily flow of TPAN, as measured at the duodenal cannula at both dilution rates were equivalent to only 41% of the flow of TPAN as predicted from measurements of rumen outflow, indicating that a substantial proportion of rumen protozoal protein was retained within the rumen."}
{"id": "PMID:465442", "title": "The influence of vitamin A status on the response of chickens to aflatoxin B1 and changes in liver lipid metabolism associated with aflatoxicosis.", "content": "1 A series of experiments were conducted to investigate the effects of dietary retinol status on chickens ingesting aflatoxin B1. The effects of dietary supplementation with biotin and alpha-tocopherol were also examined. 2. Aflatoxin B1 levels greater than 1 mg/kg diet had a detrimental effect on 'liveability', body-weight gain, food intake and food conversion efficiency. When fed for more than 2 weeks aflatoxin increased relative liver weight and liver lipid concentration. These effects were less pronounced with avitaminotic A chickens. 3. A synergistic effect on hydropericardium development was observed between aflatoxin B1 and retinol. This effect was not observed when the dietary level of alpha-tocopherol was increased tenfold. 4. The specific activities of certain hepatic lipogenic and amino acid-metabolizing enzymes were influenced by aflatoxin ingestion. A reduction in lipogenic enzyme activity was observed before a reduction in the activities of amino acid-metabolizing enzymes. 5. Liver fatty acid composition was significantly influenced by aflatoxin B1. The extent of these changes was reduced by the inclusion of additional dietary biotin.", "contents": "The influence of vitamin A status on the response of chickens to aflatoxin B1 and changes in liver lipid metabolism associated with aflatoxicosis. 1 A series of experiments were conducted to investigate the effects of dietary retinol status on chickens ingesting aflatoxin B1. The effects of dietary supplementation with biotin and alpha-tocopherol were also examined. 2. Aflatoxin B1 levels greater than 1 mg/kg diet had a detrimental effect on 'liveability', body-weight gain, food intake and food conversion efficiency. When fed for more than 2 weeks aflatoxin increased relative liver weight and liver lipid concentration. These effects were less pronounced with avitaminotic A chickens. 3. A synergistic effect on hydropericardium development was observed between aflatoxin B1 and retinol. This effect was not observed when the dietary level of alpha-tocopherol was increased tenfold. 4. The specific activities of certain hepatic lipogenic and amino acid-metabolizing enzymes were influenced by aflatoxin ingestion. A reduction in lipogenic enzyme activity was observed before a reduction in the activities of amino acid-metabolizing enzymes. 5. Liver fatty acid composition was significantly influenced by aflatoxin B1. The extent of these changes was reduced by the inclusion of additional dietary biotin."}
{"id": "PMID:465443", "title": "Efficiency of utilization of volatile fatty acids for maintenance and energy retention by sheep.", "content": "1. Two experiments were conducted with lambs sustained entirely by intragastric infusion of volatile fatty acids (VFA), protein, minerals and vitamins. 2. In the first experiment to determine the effects of VFA on nitrogen retention four mixtures of VFA (B, C, D and E) were used containing acetic, propionic and butyric acid in the following molar proportions respectively: 45, 45 and 10; 55, 35 and 10; 65, 25 and 10; 75, 15 and 10. The level of infusion was 836 kJ/live weight0.75 per d and the design was a 4 X 4 Latin square with 14 d periods. There were no significant differences in the N balance between the different mixtures of VFA though mixture B tended to give the highest N retention. 3. Thirty-two lambs were used in the second experiment for measurements of heat production in closed-circuit respiration chambers. Six mixtures of VFA were used. These included mixtures B-E from Expt 1 and in addition two mixtures (A and F) containing acetic, propionic and butyric acid in the following molar proportions respectively: 35, 55 and 10; 85, 5 and 10. The heat production was measured both at 450 and 900 kJ/W0.75 per d, except for mixture F, where it was not possible to achieve a rate of infusion in excess of 675 kJ/W0.75 per d. 4. The energy required for maintenance was determined to be 0.45 +/- 0.02 MJ/kg live weight0.75 per d regardless of the mixture used. 5. The efficiency of utilization for fattening (kf) values for the six mixtures were 0.78, 0.64, 0.57, 0.61, 0.61 and 0.59 for mixtures A, B, C, D, E and F respectively. Only mixture A was significantly better utilized than the other mixtures. This mixture also gave the most efficient N utilization. 6. It is concluded from this evidence that differences in kf for diets normally given to ruminants cannot be attributed to differences in utilization of volatile fatty acids.", "contents": "Efficiency of utilization of volatile fatty acids for maintenance and energy retention by sheep. 1. Two experiments were conducted with lambs sustained entirely by intragastric infusion of volatile fatty acids (VFA), protein, minerals and vitamins. 2. In the first experiment to determine the effects of VFA on nitrogen retention four mixtures of VFA (B, C, D and E) were used containing acetic, propionic and butyric acid in the following molar proportions respectively: 45, 45 and 10; 55, 35 and 10; 65, 25 and 10; 75, 15 and 10. The level of infusion was 836 kJ/live weight0.75 per d and the design was a 4 X 4 Latin square with 14 d periods. There were no significant differences in the N balance between the different mixtures of VFA though mixture B tended to give the highest N retention. 3. Thirty-two lambs were used in the second experiment for measurements of heat production in closed-circuit respiration chambers. Six mixtures of VFA were used. These included mixtures B-E from Expt 1 and in addition two mixtures (A and F) containing acetic, propionic and butyric acid in the following molar proportions respectively: 35, 55 and 10; 85, 5 and 10. The heat production was measured both at 450 and 900 kJ/W0.75 per d, except for mixture F, where it was not possible to achieve a rate of infusion in excess of 675 kJ/W0.75 per d. 4. The energy required for maintenance was determined to be 0.45 +/- 0.02 MJ/kg live weight0.75 per d regardless of the mixture used. 5. The efficiency of utilization for fattening (kf) values for the six mixtures were 0.78, 0.64, 0.57, 0.61, 0.61 and 0.59 for mixtures A, B, C, D, E and F respectively. Only mixture A was significantly better utilized than the other mixtures. This mixture also gave the most efficient N utilization. 6. It is concluded from this evidence that differences in kf for diets normally given to ruminants cannot be attributed to differences in utilization of volatile fatty acids."}
{"id": "PMID:465444", "title": "The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acid and protein.", "content": "A technique is described for feeding young sheep for long periods entirely by the infusion of volatile fatty acids into the rumen and protein and other essential nutrients into the abomasum. When the energy infused was twice the estimated requirement for maintenance, growth rate and nitrogen retention were essentially the same as in normally-fed sheep.", "contents": "The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acid and protein. A technique is described for feeding young sheep for long periods entirely by the infusion of volatile fatty acids into the rumen and protein and other essential nutrients into the abomasum. When the energy infused was twice the estimated requirement for maintenance, growth rate and nitrogen retention were essentially the same as in normally-fed sheep."}
{"id": "PMID:465445", "title": "The effects of pregnancy and lactation on the activities of trypsin and alpha-chymotrypsin in the rat pancreas.", "content": "1. The food intake, pancreas weight and trypsin (EC 3.4.21.4) and alpha-chymotrypsin (EC 3.4.21.1) activities in the pancreas were measured in rats during pregnancy and lactation and after the young were weaned. 2. All the quantities measured increased significantly during lactation and had returned to their original values by 4 weeks after weaning. Food intake and pancreas weight were highest after the second week of lactation. Total trypsin and alpha-chymotrypsin activity, and the activity per g tissue, fell during pregnancy and rose during lactation, reaching a maximum 1 week after weaning. 3. From these and other results it is suggested that the hypertrophy and hypersecretion of pregnancy and lactation are initiated by changes insulin secretion and mediated by the trophic effects of gut hormones, and that differences in the nature and timing of the response may be controlled by nutrient availability.", "contents": "The effects of pregnancy and lactation on the activities of trypsin and alpha-chymotrypsin in the rat pancreas. 1. The food intake, pancreas weight and trypsin (EC 3.4.21.4) and alpha-chymotrypsin (EC 3.4.21.1) activities in the pancreas were measured in rats during pregnancy and lactation and after the young were weaned. 2. All the quantities measured increased significantly during lactation and had returned to their original values by 4 weeks after weaning. Food intake and pancreas weight were highest after the second week of lactation. Total trypsin and alpha-chymotrypsin activity, and the activity per g tissue, fell during pregnancy and rose during lactation, reaching a maximum 1 week after weaning. 3. From these and other results it is suggested that the hypertrophy and hypersecretion of pregnancy and lactation are initiated by changes insulin secretion and mediated by the trophic effects of gut hormones, and that differences in the nature and timing of the response may be controlled by nutrient availability."}
{"id": "PMID:465446", "title": "Ulcerative dermatitis in rats with over fifteen generations of protein malnutrition.", "content": "1. Male and female rats with histories of up to twenty generations of protein malnutrition were found to be at a higher risk for the development of ulcerative dermatitis than rats maintained on a low-protein diet for one generation or in controls on an adequate-protein intake. 2. In all groups, female rats were more likely to have dermatitis than male rats. 3. Bacteriologic examination was performed in the intergenerationally malnourished and control animals; Staphylococcus aureus was isolated from the skins of animals in both groups, whether or not any lesion was present. In these two groups of animals, experimental inoculation with S. aureus produced dermatitis only in the malnourished animals.", "contents": "Ulcerative dermatitis in rats with over fifteen generations of protein malnutrition. 1. Male and female rats with histories of up to twenty generations of protein malnutrition were found to be at a higher risk for the development of ulcerative dermatitis than rats maintained on a low-protein diet for one generation or in controls on an adequate-protein intake. 2. In all groups, female rats were more likely to have dermatitis than male rats. 3. Bacteriologic examination was performed in the intergenerationally malnourished and control animals; Staphylococcus aureus was isolated from the skins of animals in both groups, whether or not any lesion was present. In these two groups of animals, experimental inoculation with S. aureus produced dermatitis only in the malnourished animals."}
{"id": "PMID:465447", "title": "A comparison of the influence of breast-feeding and bottle-feeding on the fatty acid composition of the erythrocytes.", "content": "The proportion of long-chain polyunsaturated derivatives of linoleic and linolenic acids in the erythrocyte lipids of infants fed a cow's milk formula was lower than in breast-fed infants. Although th formula provided only 0.55% of the total energy as linoleic acid, essential fatty acid deficiency was not detected; the triene:tetraene ratio was 0.09.", "contents": "A comparison of the influence of breast-feeding and bottle-feeding on the fatty acid composition of the erythrocytes. The proportion of long-chain polyunsaturated derivatives of linoleic and linolenic acids in the erythrocyte lipids of infants fed a cow's milk formula was lower than in breast-fed infants. Although th formula provided only 0.55% of the total energy as linoleic acid, essential fatty acid deficiency was not detected; the triene:tetraene ratio was 0.09."}
{"id": "PMID:465450", "title": "Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.", "content": "After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined as Gln-Glu-Leu-Ala-Ser-Phe-His-Ser-Val-Ser-Lsy(Pxy)-Gly-Phe-Met-Gly-Glu-Cys-Gly-Phe-Arg.", "contents": "Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase. After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined as Gln-Glu-Leu-Ala-Ser-Phe-His-Ser-Val-Ser-Lsy(Pxy)-Gly-Phe-Met-Gly-Glu-Cys-Gly-Phe-Arg."}
{"id": "PMID:465454", "title": "Synthesis and spectral properties of a hydrophobic fluorescent probe: 6-propionyl-2-(dimethylamino)naphthalene.", "content": "Environmentally sensitive fluorescent probes involve two groups, an electron donor and an electron acceptor, attached to an aromatic ring system, and maximal effects may be expected when these groups are as far apart as feasible. The syntheisis, characterization, and spectroscopic properties of 6-propionyl-2-(dimethylamino)naphthalene (PRODAN), a compound that fulfills these conditions, are described. The maximum of emission is at 401 nm in cyclohexane solution and at 531 nm in water solution, indicating an increase of dipole moment of approximately 20 D units on excitation to the lowest singlet state. The effect of temperature upon the spectral distribution and the bandwidth of fluorescence of PRODAN in 1:1 complexes with albumin shows the existence of a dynamic relaxation process of the protein surroundings within th 2-4 ns of the fluorescence lifetime.", "contents": "Synthesis and spectral properties of a hydrophobic fluorescent probe: 6-propionyl-2-(dimethylamino)naphthalene. Environmentally sensitive fluorescent probes involve two groups, an electron donor and an electron acceptor, attached to an aromatic ring system, and maximal effects may be expected when these groups are as far apart as feasible. The syntheisis, characterization, and spectroscopic properties of 6-propionyl-2-(dimethylamino)naphthalene (PRODAN), a compound that fulfills these conditions, are described. The maximum of emission is at 401 nm in cyclohexane solution and at 531 nm in water solution, indicating an increase of dipole moment of approximately 20 D units on excitation to the lowest singlet state. The effect of temperature upon the spectral distribution and the bandwidth of fluorescence of PRODAN in 1:1 complexes with albumin shows the existence of a dynamic relaxation process of the protein surroundings within th 2-4 ns of the fluorescence lifetime."}
{"id": "PMID:465456", "title": "Heat capacity microcalorimetry of the in vitro reconstitution of calf brain microtubules.", "content": "The self-assembly of calf brain tubulin, purified by the modified Weisenberg procedure, was examined in an adiabatic differential heat capacity microcalorimeter. Tubulin solutions at concentrations between 6 and 17 mg/mL were heated from 8 to 40 degrees C at heating rates between 0.1 and 1.0 deg/min in a pH 7.0 phosphate buffer containing 1 X 10(-3) M GTP, 1.6 X 10(-2) M MgCl2, and 3.4 M glycerol. The heat capacity change, deltaCp of the microtubule growth reaction was found to be -1600 +/- 500 cal/(deg mol) per 110 000 molecular weight tubulin dimer incorporated into microtubules, in agreement with the reported van't Hoff deltaCp value of -1500 cal/(deg mol) [Lee, J.C., & Timasheff, S.N. (1977) Biochemistry 16, 1754-1765]. The assembly reaction is characterized by a complex heat uptake pattern comprising both endothermic and exothermic processes.", "contents": "Heat capacity microcalorimetry of the in vitro reconstitution of calf brain microtubules. The self-assembly of calf brain tubulin, purified by the modified Weisenberg procedure, was examined in an adiabatic differential heat capacity microcalorimeter. Tubulin solutions at concentrations between 6 and 17 mg/mL were heated from 8 to 40 degrees C at heating rates between 0.1 and 1.0 deg/min in a pH 7.0 phosphate buffer containing 1 X 10(-3) M GTP, 1.6 X 10(-2) M MgCl2, and 3.4 M glycerol. The heat capacity change, deltaCp of the microtubule growth reaction was found to be -1600 +/- 500 cal/(deg mol) per 110 000 molecular weight tubulin dimer incorporated into microtubules, in agreement with the reported van't Hoff deltaCp value of -1500 cal/(deg mol) [Lee, J.C., & Timasheff, S.N. (1977) Biochemistry 16, 1754-1765]. The assembly reaction is characterized by a complex heat uptake pattern comprising both endothermic and exothermic processes."}
{"id": "PMID:465462", "title": "Measurements of fatty acid synthesis by incorporation of deuterium from deuterated water.", "content": "Fatty acid synthesis by perfused livers of rat is measured by using D2O as tracer. The newly synthesized, deuterium-labeled fatty acids are separated from unlabeled fatty acids by gas chromatography using glass capillary columns. The areas of the deuterium-labeled peaks are proportional to the amounts of fatty acids synthesized. The absolute amounts of the individual fatty acids synthesized are obtained by use of an internal standard. The number of deuterium atoms incorporated, as determined by mass spectrometry, is proportional to the D2O concentration of the perfusate, except at very high concentrations of D2O. The relative retention times of the newly synthesized, deuterium-labeled fatty acids are proportional to their deuterium content.", "contents": "Measurements of fatty acid synthesis by incorporation of deuterium from deuterated water. Fatty acid synthesis by perfused livers of rat is measured by using D2O as tracer. The newly synthesized, deuterium-labeled fatty acids are separated from unlabeled fatty acids by gas chromatography using glass capillary columns. The areas of the deuterium-labeled peaks are proportional to the amounts of fatty acids synthesized. The absolute amounts of the individual fatty acids synthesized are obtained by use of an internal standard. The number of deuterium atoms incorporated, as determined by mass spectrometry, is proportional to the D2O concentration of the perfusate, except at very high concentrations of D2O. The relative retention times of the newly synthesized, deuterium-labeled fatty acids are proportional to their deuterium content."}
{"id": "PMID:465463", "title": "Mechanism of enhancement of polynucleotide binding to cells by mutagens.", "content": "The binding of polyuridylate to cells is substantially increased by proflavine. This enhanced binding is saturable with respect to time and to the concentration of both proflavine and polyuridylate. Enhancement is observed only when cells are exposed to both proflavine and polyuridylate together and depends cooperatively on the proflavine concentration. The resulting complex formed between the cell, proflavine, and polyuridylate can be dissociated with salt but not with sucrose solutions. An increase in the binding of polyuridylate to cells similar to that observed with proflavine was also obtained with cationic dyes such as acridine orange, 9-aminoacridine, and Hoechst 33258, while the introduction of a bulky polysaccharide residue, dextran, into the dyes cancels these effects. Similarly, cationic aromatic compounds such as primaquine and quinacrine which carry bulky nonplanar substituents or aliphatic cationic compounds like ethylenediamine do not enhance binding. Proflavine is unable to augment the binding of a basic macromolecule, diethylaminoethylaminoethyldextran, to cells. The model proposed for the enhanced binding of polyuridylate is based on the cooperative formation of stacked complexes of cationic dye located between the cell surface and the bound polyuridylate.", "contents": "Mechanism of enhancement of polynucleotide binding to cells by mutagens. The binding of polyuridylate to cells is substantially increased by proflavine. This enhanced binding is saturable with respect to time and to the concentration of both proflavine and polyuridylate. Enhancement is observed only when cells are exposed to both proflavine and polyuridylate together and depends cooperatively on the proflavine concentration. The resulting complex formed between the cell, proflavine, and polyuridylate can be dissociated with salt but not with sucrose solutions. An increase in the binding of polyuridylate to cells similar to that observed with proflavine was also obtained with cationic dyes such as acridine orange, 9-aminoacridine, and Hoechst 33258, while the introduction of a bulky polysaccharide residue, dextran, into the dyes cancels these effects. Similarly, cationic aromatic compounds such as primaquine and quinacrine which carry bulky nonplanar substituents or aliphatic cationic compounds like ethylenediamine do not enhance binding. Proflavine is unable to augment the binding of a basic macromolecule, diethylaminoethylaminoethyldextran, to cells. The model proposed for the enhanced binding of polyuridylate is based on the cooperative formation of stacked complexes of cationic dye located between the cell surface and the bound polyuridylate."}
{"id": "PMID:465471", "title": "Time-dependent changes in the size distribution of distearoylphosphatidylcholine vesicles.", "content": "The results of transmission electron microscopic and ultracentrifugal studies of the size distributions of sonicated distearoylphosphatidylcholine vesicles are reported. Small vesicles (d approximately 300 A) were prepared by sonication of pure 1,2-distearoyl-3-sn-phosphatidylcholine in water and incubated at 4, 21, 40, 53, and 65 degrees C. The vesicle size distributions changed as a function of time at all temperatures below the phase-transition temperature but remained constant at the transition temperature and above. The sizes of structures to which the small vesicles are converted are the same at all temperatures, although the rates of conversion differ. The primary structures formed are identified as larger vesicles. The rate of loss of small vesicles is found to increase with decreasing temperature. At 4 and 21 degrees C small vesicles are converted to amorphous material, possibly irregular fragments of neat phase, in addition to being converted to larger vesicles. Trace amounts of an impurity commonly produced in the synthesis of 1,2-distearoyl-3-sn-phosphatidylcholine, 1,3-distearoyl-2-sn-phosphatidylcholine, are found to dramatically reduce the rate of loss of small vesicles at 21 degrees C.", "contents": "Time-dependent changes in the size distribution of distearoylphosphatidylcholine vesicles. The results of transmission electron microscopic and ultracentrifugal studies of the size distributions of sonicated distearoylphosphatidylcholine vesicles are reported. Small vesicles (d approximately 300 A) were prepared by sonication of pure 1,2-distearoyl-3-sn-phosphatidylcholine in water and incubated at 4, 21, 40, 53, and 65 degrees C. The vesicle size distributions changed as a function of time at all temperatures below the phase-transition temperature but remained constant at the transition temperature and above. The sizes of structures to which the small vesicles are converted are the same at all temperatures, although the rates of conversion differ. The primary structures formed are identified as larger vesicles. The rate of loss of small vesicles is found to increase with decreasing temperature. At 4 and 21 degrees C small vesicles are converted to amorphous material, possibly irregular fragments of neat phase, in addition to being converted to larger vesicles. Trace amounts of an impurity commonly produced in the synthesis of 1,2-distearoyl-3-sn-phosphatidylcholine, 1,3-distearoyl-2-sn-phosphatidylcholine, are found to dramatically reduce the rate of loss of small vesicles at 21 degrees C."}
{"id": "PMID:465472", "title": "Folding pathways of immunoglobulin domains. The folding kinetics of the Cgamma3 domain of human IgG1.", "content": "The in vitro folding kinetics of a fragment corresponding to an intact dimer of the Cgamma3 domain of human IgG1 (pFc') were monitored via the large changes in tryptophan fluorescence which accompany these processes. In going from the guanidine hydrochloride (Gdn.HCl) induced unfolded state (4.0 M Gdn.HCl) to the native state (0.5 M Gdn.HCl), three well-separated first-order processes were observed having time constants of 5, 50, and 350 s and roughly equal amplitudes. These values were concentration independent, a fact consistent with there being no fluorescence change accompanying dimerization. These time constants are one to two orders of magnitude slower than those observed for proteins of similar size such as ribonuclease or cytochrome c, most probably reflecting the complex processes involved in forming the correct beta-sheet arrangement of immunoglobulin domains. The corresponding unfolding transition is biphasic having time constant values of 50 and 500 s, the latter comprising 80% of the fluorescence change. These data indicate the presence of at least one species with intermediate fluorescence along the unfolding pathway. Gdn.HCl concentration jumps were also performed over various intervals within the transition zone. The results are not consistent with a fully reversible mechanism. In the absence of the intrachain disulfide bond, pFc' exists in an unfolded state even at 0.5 M Gdn.HCl. In a concomitant refolding and reoxidation experiment (at 0.5 M Gdn.HCl and using an optimal disulfide interchange catalytic system), the time constant for disulfide formation was in the range of 80--200 s and the fluorescence change revealed a lag phase analyzable in terms of rate-limiting reoxidation and refolding times consistent with those observed for the initially disulfide bonded species. Under similar conditions but a 4 M Gdn.HCl, reoxidation was more than two orders of magnitude slower, suggesting that reoxidation is directed by a refolding nucleation event.", "contents": "Folding pathways of immunoglobulin domains. The folding kinetics of the Cgamma3 domain of human IgG1. The in vitro folding kinetics of a fragment corresponding to an intact dimer of the Cgamma3 domain of human IgG1 (pFc') were monitored via the large changes in tryptophan fluorescence which accompany these processes. In going from the guanidine hydrochloride (Gdn.HCl) induced unfolded state (4.0 M Gdn.HCl) to the native state (0.5 M Gdn.HCl), three well-separated first-order processes were observed having time constants of 5, 50, and 350 s and roughly equal amplitudes. These values were concentration independent, a fact consistent with there being no fluorescence change accompanying dimerization. These time constants are one to two orders of magnitude slower than those observed for proteins of similar size such as ribonuclease or cytochrome c, most probably reflecting the complex processes involved in forming the correct beta-sheet arrangement of immunoglobulin domains. The corresponding unfolding transition is biphasic having time constant values of 50 and 500 s, the latter comprising 80% of the fluorescence change. These data indicate the presence of at least one species with intermediate fluorescence along the unfolding pathway. Gdn.HCl concentration jumps were also performed over various intervals within the transition zone. The results are not consistent with a fully reversible mechanism. In the absence of the intrachain disulfide bond, pFc' exists in an unfolded state even at 0.5 M Gdn.HCl. In a concomitant refolding and reoxidation experiment (at 0.5 M Gdn.HCl and using an optimal disulfide interchange catalytic system), the time constant for disulfide formation was in the range of 80--200 s and the fluorescence change revealed a lag phase analyzable in terms of rate-limiting reoxidation and refolding times consistent with those observed for the initially disulfide bonded species. Under similar conditions but a 4 M Gdn.HCl, reoxidation was more than two orders of magnitude slower, suggesting that reoxidation is directed by a refolding nucleation event."}
{"id": "PMID:465473", "title": "Contributions by ionic and steric features of ligands to their binding with phosphorylcholine-specific immunoglobulin IgA H-8 as determined by fluorescence spectroscopy.", "content": "The murine myeloma IgA H-8 Fab' fragment which exhibits a binding specificity for phosphorylcholine was assayed for its ability to bind with a number of charged ligands. Monitoring of the ligand-induced changes of protein fluorescence provided a fast and accurate method of determining the equilibrium binding constants. The binding data along with fluorescence spectral properties of the protein permitted an assessment of the relative importance of some binding parameters as well as an evaluation of certain ionic and steric contributions made by ligands exhibiting significant binding affinity for the antibody fragment. Among the conclusions reached is that the dielectric of the binding site microenvironment is important in determining the strength of binding and that hydrophobic groups surrounding a quaternary cationic ligand are important in creating an appropriate binding site of low dielectric value.", "contents": "Contributions by ionic and steric features of ligands to their binding with phosphorylcholine-specific immunoglobulin IgA H-8 as determined by fluorescence spectroscopy. The murine myeloma IgA H-8 Fab' fragment which exhibits a binding specificity for phosphorylcholine was assayed for its ability to bind with a number of charged ligands. Monitoring of the ligand-induced changes of protein fluorescence provided a fast and accurate method of determining the equilibrium binding constants. The binding data along with fluorescence spectral properties of the protein permitted an assessment of the relative importance of some binding parameters as well as an evaluation of certain ionic and steric contributions made by ligands exhibiting significant binding affinity for the antibody fragment. Among the conclusions reached is that the dielectric of the binding site microenvironment is important in determining the strength of binding and that hydrophobic groups surrounding a quaternary cationic ligand are important in creating an appropriate binding site of low dielectric value."}
{"id": "PMID:465474", "title": "Organization and heterogeneity of sequences within a repeating unit of human Y chromosome deoxyribonucleic acid.", "content": "Fragments of 3.4 kilobases (kb) are released from DNA of human males, but not DNA of human females, by cleavage with restriction endonucleases HaeIII, EcoRI, or EcoRII. Most, if not all, reiterated DNA which is specific for the Y chromosome (it-Y DNA) is present within these male-specific 3.4-kb molecules. Although such 3.4-kb molecules are themselves localized to the Y chromosome, this is not true for all sequences within them. At least two distinguishable types of reiterated sequences are found within each 3.4-kb molecule. One type consists of at least two families which are highly reiterated and are not confined to the Y chromosome. The other type is composed of an estimated minimum of 39 families, each moderately reiterated and localized to the Y chromosome. Y-specific and non-Y-specific sequences are interspersed with one another in the same 3.4-kb molecule. In the average 3.4-kb molecule, three 800 nucleotide lengths of Y-specific sequences alternate with four 250 nucleotide lengths of non-Y-specific sequences. Since the total number of families of Y-specific sequences, calculated on the basis of reiteration frequency and total abundance in a male genome, greatly exceeds the number of Y -specific sequences present in a single 3.4-kb molecule, it necessarily follows that the population of these 3.4-kb molecules is heterogeneous.", "contents": "Organization and heterogeneity of sequences within a repeating unit of human Y chromosome deoxyribonucleic acid. Fragments of 3.4 kilobases (kb) are released from DNA of human males, but not DNA of human females, by cleavage with restriction endonucleases HaeIII, EcoRI, or EcoRII. Most, if not all, reiterated DNA which is specific for the Y chromosome (it-Y DNA) is present within these male-specific 3.4-kb molecules. Although such 3.4-kb molecules are themselves localized to the Y chromosome, this is not true for all sequences within them. At least two distinguishable types of reiterated sequences are found within each 3.4-kb molecule. One type consists of at least two families which are highly reiterated and are not confined to the Y chromosome. The other type is composed of an estimated minimum of 39 families, each moderately reiterated and localized to the Y chromosome. Y-specific and non-Y-specific sequences are interspersed with one another in the same 3.4-kb molecule. In the average 3.4-kb molecule, three 800 nucleotide lengths of Y-specific sequences alternate with four 250 nucleotide lengths of non-Y-specific sequences. Since the total number of families of Y-specific sequences, calculated on the basis of reiteration frequency and total abundance in a male genome, greatly exceeds the number of Y -specific sequences present in a single 3.4-kb molecule, it necessarily follows that the population of these 3.4-kb molecules is heterogeneous."}
{"id": "PMID:465476", "title": "Interaction between wheat germ RNA polymerase II and adenovirus 2 DNA. Evidence for two types of stable binary complexes.", "content": "Transcription of Adenovirus 2 DNA (Ad 2 DNA) by wheat germ RNA polymerase II in vitro satisfies criteria that have been used to establish that Escherichia coli or coliphage transcription in vitro is initiated at true promoters. (1) Wheat germ RNA polymerase forms highly stable complexes at specific sites on Ad 2 DNA, with a Kassoc of (4--5) X 10(10) M-1. (2) Electron microscopic visualization of enzyme bound to Ad 2 DNA reveals the location of eight strong binding sites, at least five of which appear to correspond to promoters that have been identified in studies of Ad 2 transcription in vivo [Evans, R. M., Fraser, N., Ziff, E., Weber, J., Wilson, M., & Darnell, J.E. (1977) Cell 12, 733--739; Berk, A.J., & Sharp, P.A. (1977) Cell 12, 45--55; Weinmann, R., & Aiello, L. O. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 1662--1666]. (3) Transcription of Ad 2 DNA from preformed complexes with wheat germ polymerase is capable of escaping the action of rifamycin AF/013 and is relatively resistant to polyriboinosinic acid. In addition, our results are consistent with a two-state model for the interaction of wheat germ RNA polymerase with Ad 2 DNA, indicating that the mechansisms of transcription initiation and promoter-site selection in eucaryotes may be very similar to mechanisms elucidated in procaryotic systems.", "contents": "Interaction between wheat germ RNA polymerase II and adenovirus 2 DNA. Evidence for two types of stable binary complexes. Transcription of Adenovirus 2 DNA (Ad 2 DNA) by wheat germ RNA polymerase II in vitro satisfies criteria that have been used to establish that Escherichia coli or coliphage transcription in vitro is initiated at true promoters. (1) Wheat germ RNA polymerase forms highly stable complexes at specific sites on Ad 2 DNA, with a Kassoc of (4--5) X 10(10) M-1. (2) Electron microscopic visualization of enzyme bound to Ad 2 DNA reveals the location of eight strong binding sites, at least five of which appear to correspond to promoters that have been identified in studies of Ad 2 transcription in vivo [Evans, R. M., Fraser, N., Ziff, E., Weber, J., Wilson, M., & Darnell, J.E. (1977) Cell 12, 733--739; Berk, A.J., & Sharp, P.A. (1977) Cell 12, 45--55; Weinmann, R., & Aiello, L. O. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 1662--1666]. (3) Transcription of Ad 2 DNA from preformed complexes with wheat germ polymerase is capable of escaping the action of rifamycin AF/013 and is relatively resistant to polyriboinosinic acid. In addition, our results are consistent with a two-state model for the interaction of wheat germ RNA polymerase with Ad 2 DNA, indicating that the mechansisms of transcription initiation and promoter-site selection in eucaryotes may be very similar to mechanisms elucidated in procaryotic systems."}
{"id": "PMID:465478", "title": "Amino acid sequence and sites of phosphorylation in a highly acidic region of nucleolar nonhistone protein C23.", "content": "Novikoff hepatoma nucleolar nonhistone proteins, C23 and B23, contain highly acidic phosphorylated regions (Mamrack, M. D., et al. (1977) Biochem. Biophys. Res. Commun. 76, 150--157). Tryptic peptides from protein C23 containing these regions were purified by DEAE-Sephadex columns and paper electrophoresis at pH 1.8. One of these, peptide C23-Ca, was sequenced by combined automated and conventional methods. The proposed amino acid sequence is shown in eq 1. This peptide was found in three 32P-labeled forms with phosphoryl groups at positions 8 and 25, and probably 28. The highly acidic sequences adjacent to the phosphorylation sites represent a unique class of phosphorylation sites different from those in histones or substrates for cytoplasmic cAMP-dependent kinases. Ala-Ala-Pro-Ala-A5la-Pro-Ala-Ser-Glu-A10sp-Glu-Asp-Glu-Glu-A15sp-Asp-Asp-Asp-Glu-A20sp-Asp-Asp-Asp-Asp-S25er-Gln-Glu-Ser-Glu-G30lu-Glu-Asp-Glu-Glu-V35al-Met-Glu-Ile-Thr-P40ro-Ala-Lys (1).", "contents": "Amino acid sequence and sites of phosphorylation in a highly acidic region of nucleolar nonhistone protein C23. Novikoff hepatoma nucleolar nonhistone proteins, C23 and B23, contain highly acidic phosphorylated regions (Mamrack, M. D., et al. (1977) Biochem. Biophys. Res. Commun. 76, 150--157). Tryptic peptides from protein C23 containing these regions were purified by DEAE-Sephadex columns and paper electrophoresis at pH 1.8. One of these, peptide C23-Ca, was sequenced by combined automated and conventional methods. The proposed amino acid sequence is shown in eq 1. This peptide was found in three 32P-labeled forms with phosphoryl groups at positions 8 and 25, and probably 28. The highly acidic sequences adjacent to the phosphorylation sites represent a unique class of phosphorylation sites different from those in histones or substrates for cytoplasmic cAMP-dependent kinases. Ala-Ala-Pro-Ala-A5la-Pro-Ala-Ser-Glu-A10sp-Glu-Asp-Glu-Glu-A15sp-Asp-Asp-Asp-Glu-A20sp-Asp-Asp-Asp-Asp-S25er-Gln-Glu-Ser-Glu-G30lu-Glu-Asp-Glu-Glu-V35al-Met-Glu-Ile-Thr-P40ro-Ala-Lys (1)."}
{"id": "PMID:465480", "title": "Subunit structure and peptide mapping of junctional and extrajunctional acetylcholine receptors from rat muscle.", "content": "We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis. The junctional form of the acetylcholine receptor was purified from normal leg muscle by affinity chromatography on concanavalin A/Sepharose and cobrotoxin/Sepharose followed by sucrose gradient centrifugation. Analysis of radioiodinated receptor by polyacrylamide gel electrophoresis in sodium dodecyl sulfate indicated that the subunit structure of the junctional receptor was similar to that previously determined for the extra-junctional form (Froehner, S. C., et al. (1977) J. Biol. Chem. 252, 8589-8596), with major polypeptides, whose apparent molecular weights in 9% polyacrylamide gels were 45 000 and 51 000. In addition, several minor polypeptides were found. When the two receptors were labeled with different isotopes of iodine and run together on a sodium dodecyl sulfate gel, the subunits of one receptor could not be resolved from those of the other. As seen earlier with the extrajunctional form, the affinity alkylating reagent [3H]MBTA labeled the 45 000- and 49 000-dalton polypeptides of the junctional receptor. Peptide mapping showed that the two MBTA binding subunits are structurally related, although they are unrelated to the other polypeptides, and that the 45 000- and 51 000-dalton polypeptides of the junctional receptor were indistinguishable from those of the extrajunctional receptor. In addition, peptide mapping of the four subunits of acetylcholine receptor isolated from Torpedo californica electric organ showed that these four polypeptides appear to be structurally unrelated.", "contents": "Subunit structure and peptide mapping of junctional and extrajunctional acetylcholine receptors from rat muscle. We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis. The junctional form of the acetylcholine receptor was purified from normal leg muscle by affinity chromatography on concanavalin A/Sepharose and cobrotoxin/Sepharose followed by sucrose gradient centrifugation. Analysis of radioiodinated receptor by polyacrylamide gel electrophoresis in sodium dodecyl sulfate indicated that the subunit structure of the junctional receptor was similar to that previously determined for the extra-junctional form (Froehner, S. C., et al. (1977) J. Biol. Chem. 252, 8589-8596), with major polypeptides, whose apparent molecular weights in 9% polyacrylamide gels were 45 000 and 51 000. In addition, several minor polypeptides were found. When the two receptors were labeled with different isotopes of iodine and run together on a sodium dodecyl sulfate gel, the subunits of one receptor could not be resolved from those of the other. As seen earlier with the extrajunctional form, the affinity alkylating reagent [3H]MBTA labeled the 45 000- and 49 000-dalton polypeptides of the junctional receptor. Peptide mapping showed that the two MBTA binding subunits are structurally related, although they are unrelated to the other polypeptides, and that the 45 000- and 51 000-dalton polypeptides of the junctional receptor were indistinguishable from those of the extrajunctional receptor. In addition, peptide mapping of the four subunits of acetylcholine receptor isolated from Torpedo californica electric organ showed that these four polypeptides appear to be structurally unrelated."}
{"id": "PMID:465481", "title": "Steady-state kinetics of mouse DNA polymerase beta.", "content": "DNA polymerase beta from mouse myeloma has been purified to near homogeneity, and its properties have been examined. The enzyme did not catalyze a detectable level of dNTP turnover, pyrophosphate exchange, pyrophosphorolysis, 3'-exonuclease degradation, or 5'-exonuclease degradation. Steady-state kinetic studies point to an ordered bibi mechanism for the polymerization reaction. Metal activation, which is required for polymerization, did not alter the Km for either the dNTP or the template--primer.", "contents": "Steady-state kinetics of mouse DNA polymerase beta. DNA polymerase beta from mouse myeloma has been purified to near homogeneity, and its properties have been examined. The enzyme did not catalyze a detectable level of dNTP turnover, pyrophosphate exchange, pyrophosphorolysis, 3'-exonuclease degradation, or 5'-exonuclease degradation. Steady-state kinetic studies point to an ordered bibi mechanism for the polymerization reaction. Metal activation, which is required for polymerization, did not alter the Km for either the dNTP or the template--primer."}
{"id": "PMID:465483", "title": "Cytochrome function in the cyclic electron transport pathway of chloroplasts.", "content": "Flash excitation of isolated intact chloroplasts promoted absorbance transients corresponding to the electrochromic effect (P-518) and the alpha-bands of cytochrome b6 and cytochrome f. Under conditions supporting coupled cyclic electron flow, the oxidation of cytochrome b6 and the reduction of cytochrome f had relaxation half-times of 15 and 17 ms, respectively. Optimal poising of cyclic electron flow, achieved by addition of 0.1 microM 3-(3,4-dichlorophenyl)-1,1-dimethylurea, increased phosphorylation of endogenous ADP and prolonged these relaxation times. The presence of NH4Cl, or monensin plus NaCl, decreased the half-times for cytochrome relaxation to approximately 2 ms. Uncouplers also revealed the presence of a slow rise component in the electrochromic absorption shift with formation half-time of about 2 ms. Ths inhibitors of cyclic phosphorylation antimycin and 2,5-dibromo-3-methyl-6-isoprophy-p-benzoquinone abolished the slow rise in the electrochromic shift and prolonged the uncoupled relaxation times of cytochromes b6 and f by factors of ten or more. These observations indicate that cytochrome b6, plastoquinone and cytochrome f participated in a coupled electron transport process responsible for cyclic phosphorylation in intact chloroplasts. Estimations of cyclic phosphorylation rates from 40 to 120 mumol ATP/mg chlorophyll per h suggest that this process can provide a substantial fraction of the ATP needed for CO2 fixation.", "contents": "Cytochrome function in the cyclic electron transport pathway of chloroplasts. Flash excitation of isolated intact chloroplasts promoted absorbance transients corresponding to the electrochromic effect (P-518) and the alpha-bands of cytochrome b6 and cytochrome f. Under conditions supporting coupled cyclic electron flow, the oxidation of cytochrome b6 and the reduction of cytochrome f had relaxation half-times of 15 and 17 ms, respectively. Optimal poising of cyclic electron flow, achieved by addition of 0.1 microM 3-(3,4-dichlorophenyl)-1,1-dimethylurea, increased phosphorylation of endogenous ADP and prolonged these relaxation times. The presence of NH4Cl, or monensin plus NaCl, decreased the half-times for cytochrome relaxation to approximately 2 ms. Uncouplers also revealed the presence of a slow rise component in the electrochromic absorption shift with formation half-time of about 2 ms. Ths inhibitors of cyclic phosphorylation antimycin and 2,5-dibromo-3-methyl-6-isoprophy-p-benzoquinone abolished the slow rise in the electrochromic shift and prolonged the uncoupled relaxation times of cytochromes b6 and f by factors of ten or more. These observations indicate that cytochrome b6, plastoquinone and cytochrome f participated in a coupled electron transport process responsible for cyclic phosphorylation in intact chloroplasts. Estimations of cyclic phosphorylation rates from 40 to 120 mumol ATP/mg chlorophyll per h suggest that this process can provide a substantial fraction of the ATP needed for CO2 fixation."}
{"id": "PMID:465484", "title": "Dicarboxylate transport in the inner membrane matrix fraction obtained from rat liver mitochondria.", "content": "Dicarboxylate transport was studied in the inner membrane matrix fraction (mitoplasts) and compared to that in intact rat-liver mitochondria from which the former was obtained. It is concluded that, kinetics of dicarboxylate exchange measured in mitoplasts, are very similar to those observed with mitochondria. These results would indicate that the preparation technique preserves the integrity of the inner membrane and that neither the outer membrane nor the components of the peripheral space affect these results.", "contents": "Dicarboxylate transport in the inner membrane matrix fraction obtained from rat liver mitochondria. Dicarboxylate transport was studied in the inner membrane matrix fraction (mitoplasts) and compared to that in intact rat-liver mitochondria from which the former was obtained. It is concluded that, kinetics of dicarboxylate exchange measured in mitoplasts, are very similar to those observed with mitochondria. These results would indicate that the preparation technique preserves the integrity of the inner membrane and that neither the outer membrane nor the components of the peripheral space affect these results."}
{"id": "PMID:465486", "title": "The kinetic relationship between the C-550 absorbance change, the reduction of Q(delta A320) and the variable fluorescence yield change in chloroplasts at room temperature.", "content": "The light minus dark difference spectrum and the kinetics of the indicator pigment C-550 have been measured at room temperature in isolate, envelope-free chloroplasts in the presence of 3-(3' ,4'-dichlorophenyl)-1,1-dimethylurea (DCMU). The C-550 spectrum indicates a band shift with peaks at 540 and 550 nm and has an isobestic point at 545 nm. On the assumption of 400 chlorophyll molecules per electron transfer chain the differentaial extinction coefficient delta epsilon (540-550) is calculated to be approximately 5 mM-1 . CM-1. The kinetics of the C-550 absorbance change, occurring upin the onset of continuous illumination, are shown to be biphasic and strictly correlated with the kinetics of the complementary area measured from the fluorescence induction curve under identical cinditions and with those of the absorbance increase at 320 nm due to photoreduction of Q. The lighted-induced change in these three parameters can be described as a function of the variable fluorescence yield change occurring under the same conditions. Such functions are non-linear and reveal a heterogeneous dependence of the variable fluorescence yield on the fraction of closed System II reaction centers. It is concluded that for every molecule of the primary electron acceptor Q of Photosystem II that is photochemically reduced there corresponds an equivalent change in the absorbance of the indicator pigment C-550 and in the size of the complementary area. Ths, C-550 and area are two valid parameters for monitoring the primary photochemical activity of System II at the room temperature.", "contents": "The kinetic relationship between the C-550 absorbance change, the reduction of Q(delta A320) and the variable fluorescence yield change in chloroplasts at room temperature. The light minus dark difference spectrum and the kinetics of the indicator pigment C-550 have been measured at room temperature in isolate, envelope-free chloroplasts in the presence of 3-(3' ,4'-dichlorophenyl)-1,1-dimethylurea (DCMU). The C-550 spectrum indicates a band shift with peaks at 540 and 550 nm and has an isobestic point at 545 nm. On the assumption of 400 chlorophyll molecules per electron transfer chain the differentaial extinction coefficient delta epsilon (540-550) is calculated to be approximately 5 mM-1 . CM-1. The kinetics of the C-550 absorbance change, occurring upin the onset of continuous illumination, are shown to be biphasic and strictly correlated with the kinetics of the complementary area measured from the fluorescence induction curve under identical cinditions and with those of the absorbance increase at 320 nm due to photoreduction of Q. The lighted-induced change in these three parameters can be described as a function of the variable fluorescence yield change occurring under the same conditions. Such functions are non-linear and reveal a heterogeneous dependence of the variable fluorescence yield on the fraction of closed System II reaction centers. It is concluded that for every molecule of the primary electron acceptor Q of Photosystem II that is photochemically reduced there corresponds an equivalent change in the absorbance of the indicator pigment C-550 and in the size of the complementary area. Ths, C-550 and area are two valid parameters for monitoring the primary photochemical activity of System II at the room temperature."}
{"id": "PMID:465487", "title": "The terminal oxidase of Photobacterium phosphoreum. A novel cytochrome.", "content": "The terminal oxidase of Photobacterium phosphoreum has been purified to the electrophoretically homogeneous state and some of its properties have been studied. The enzyme catalyses oxidation of ascorbate in the presence of phenazine methosulphate or N,N,N',N'-tetramethyl-p-phenylenediamine. The reaction is inhibited by cyanide. Nitrite at comparatively high concentrations inhibits the enzyme, but the enzyme does not catalyse nitrite reduction with ascorbate plus the electron mediator as the electron donor. The enzyme shows the absorption peaks at 632, 565, 534 and 436 nm in the reduced form. It has two kinds of haems: protohaem and haem d. Namely, the enzyme is a 'cytochrome bd'-type oxidase; a novel cytochrome.", "contents": "The terminal oxidase of Photobacterium phosphoreum. A novel cytochrome. The terminal oxidase of Photobacterium phosphoreum has been purified to the electrophoretically homogeneous state and some of its properties have been studied. The enzyme catalyses oxidation of ascorbate in the presence of phenazine methosulphate or N,N,N',N'-tetramethyl-p-phenylenediamine. The reaction is inhibited by cyanide. Nitrite at comparatively high concentrations inhibits the enzyme, but the enzyme does not catalyse nitrite reduction with ascorbate plus the electron mediator as the electron donor. The enzyme shows the absorption peaks at 632, 565, 534 and 436 nm in the reduced form. It has two kinds of haems: protohaem and haem d. Namely, the enzyme is a 'cytochrome bd'-type oxidase; a novel cytochrome."}
{"id": "PMID:465489", "title": "Effects of dibromothymoquinone on mung bean mitochondrial electron transfer and membrane fluidity.", "content": "The effects of the quinone analog dibromothymoquinone on electron transfer in isolated mung bean mitochondria are described. Both the main, cyanide-sensitive and the alternate, cyanide-insensitive pathways are inhibited by dibromothymoquinone but in markedly different fashions. Half-maximal inhibition appeared at 40 microM and 20 microM dibromothymoquinone for the cyanide-sensitive and alternate pathways, respectively. With succinate as the electron donor, dibromothymoquinone inhibited the alternate pathway at a single site; showing a mixed, non-competitive type inhibition. On the succinate, cyanide-sensitive pathway dibromothymoquinone showed two sites of inhibition and neither coincides with the site of inhibition associated with the alternate pathway. With malate as the electron donor, two sites of inhibition by dibromothymoquinone were observed regardless of the pathway measured. Dibromothymoquinone also inhibited the rate of valinomycin-induced swelling of isolated mung bean mitochondria. Steady-state kinetics showed the inhibition to be non-competitive with respect to valinomycin. Additionally dibromothymoquinone was observed to increase the fluorescence polarization associated with the hydrophobic probe 1,6-diphenylhexatriene. The results indicated that dibromothymoquinone decreased the fluidity of the inner mitochondrial membrane and suggested that the inhibition of mitochondrial electron transfer by dibromothymoquinone may be associated with this decrease in membrane fluidity. The relationship of the multisite nature of the inhibition of electron transfer by dibromothymoquinone and the possible role of mobile electron carriers such as ubiquinone on the main and alternate respiratory pathways of higher plants is discussed.", "contents": "Effects of dibromothymoquinone on mung bean mitochondrial electron transfer and membrane fluidity. The effects of the quinone analog dibromothymoquinone on electron transfer in isolated mung bean mitochondria are described. Both the main, cyanide-sensitive and the alternate, cyanide-insensitive pathways are inhibited by dibromothymoquinone but in markedly different fashions. Half-maximal inhibition appeared at 40 microM and 20 microM dibromothymoquinone for the cyanide-sensitive and alternate pathways, respectively. With succinate as the electron donor, dibromothymoquinone inhibited the alternate pathway at a single site; showing a mixed, non-competitive type inhibition. On the succinate, cyanide-sensitive pathway dibromothymoquinone showed two sites of inhibition and neither coincides with the site of inhibition associated with the alternate pathway. With malate as the electron donor, two sites of inhibition by dibromothymoquinone were observed regardless of the pathway measured. Dibromothymoquinone also inhibited the rate of valinomycin-induced swelling of isolated mung bean mitochondria. Steady-state kinetics showed the inhibition to be non-competitive with respect to valinomycin. Additionally dibromothymoquinone was observed to increase the fluorescence polarization associated with the hydrophobic probe 1,6-diphenylhexatriene. The results indicated that dibromothymoquinone decreased the fluidity of the inner mitochondrial membrane and suggested that the inhibition of mitochondrial electron transfer by dibromothymoquinone may be associated with this decrease in membrane fluidity. The relationship of the multisite nature of the inhibition of electron transfer by dibromothymoquinone and the possible role of mobile electron carriers such as ubiquinone on the main and alternate respiratory pathways of higher plants is discussed."}
{"id": "PMID:465490", "title": "Photosystem II energy coupling in chloroplasts with H2O2 as electron donor.", "content": "NH2OH-treated, non-water-splitting chloroplasts can oxidize H2O2 to O2 through Photosystem II at substantial rates (100--250 muequiv . h-1 . mg-1 chlorophyll with 5 mM H2O2) using 2,5-dimethyl-p-benzoquinone as an electron acceptor in the presence of the plastoquinone antagonist dibromothymoquinone. This H2O2 leads to Photosystem II leads to dimethylquinone reaction supports phosphorylation with a P/e2 ratio of 0.25--0.35 and proton uptake with H+/e values of 0.67 (pH 8)--0.85 (pH 6). These are close to the P/e2 value of 0.3--0.38 and the H+/e values of 0.7--0.93 found in parallel experiments for the H2O leads to Photosystem II leads to dimethylquinone reaction in untreated chloroplasts. Semi-quantitative data are also presented which show that the donor leads to Photosystem II leads to dibromothymoquinone (leads to O2) reaction can support phosphorylation when the donor used is a proton-releasing reductant (benzidine, catechol) but not when it is a non-proton carrier (I-, ferrocyanide).", "contents": "Photosystem II energy coupling in chloroplasts with H2O2 as electron donor. NH2OH-treated, non-water-splitting chloroplasts can oxidize H2O2 to O2 through Photosystem II at substantial rates (100--250 muequiv . h-1 . mg-1 chlorophyll with 5 mM H2O2) using 2,5-dimethyl-p-benzoquinone as an electron acceptor in the presence of the plastoquinone antagonist dibromothymoquinone. This H2O2 leads to Photosystem II leads to dimethylquinone reaction supports phosphorylation with a P/e2 ratio of 0.25--0.35 and proton uptake with H+/e values of 0.67 (pH 8)--0.85 (pH 6). These are close to the P/e2 value of 0.3--0.38 and the H+/e values of 0.7--0.93 found in parallel experiments for the H2O leads to Photosystem II leads to dimethylquinone reaction in untreated chloroplasts. Semi-quantitative data are also presented which show that the donor leads to Photosystem II leads to dibromothymoquinone (leads to O2) reaction can support phosphorylation when the donor used is a proton-releasing reductant (benzidine, catechol) but not when it is a non-proton carrier (I-, ferrocyanide)."}
{"id": "PMID:465491", "title": "Dependence of mammalian DNA replication on DNA supercoiling. I. Effects of ethidium bromide on DNA synthesis in permeable Chinese hamster ovary cells.", "content": "Chinese hamster ovary cells labelled with [14C]thymidine were made permeable, incubated with various concentrations of the intercalating dye ethidium bromide, and centrifuged through neutral sucrose gradients. The gradient profiles of these cells were qualitatively similar to those obtained by centrifuging DNA from untreated, lysed permeable cells through gradients containing ethidium bromide. The sedimentation distance of DNA had a biphasic dependence on the concentration of ethidium bromide, suggesting that the dye altered the amount of DNA supercoiling in situ. The effect of ethidium bromide intercalation on incorporation of [3H]dTMP into acid-precipitable material in an in vitro DNA synthesis mixture was measured. The incorporation of [3H]dTMP was unaffected by less than 1 microgram/ml of ethidium bromide, enhanced up to two-fold by 1--10 microgram/ml, and inhibited by concentrations greater than 10 micrograms/ml. Alkaline sucrose gradient analysis revealed a higher percentage of small DNA fragments (6--20 S) in the cells treated with 2 micrograms/ml ethidium bromide than in control cells. These fragments attained parental size within the same time as the fragments in control cells. In cells treated with 2 micrograms/ml ethidium bromide, a significant fraction of newly synthesized DNA resulted from new starts, whereas in untreated cells practically none of the newly synthesized DNA resulted from new starts. These results suggest that relaxation of DNA supercoiled structures ahead of the replication fork generates spurious initiations of DNA synthesis and that in intact cells the rate of chain elongation is limited by supercoiled regions ahead of the growing point.", "contents": "Dependence of mammalian DNA replication on DNA supercoiling. I. Effects of ethidium bromide on DNA synthesis in permeable Chinese hamster ovary cells. Chinese hamster ovary cells labelled with [14C]thymidine were made permeable, incubated with various concentrations of the intercalating dye ethidium bromide, and centrifuged through neutral sucrose gradients. The gradient profiles of these cells were qualitatively similar to those obtained by centrifuging DNA from untreated, lysed permeable cells through gradients containing ethidium bromide. The sedimentation distance of DNA had a biphasic dependence on the concentration of ethidium bromide, suggesting that the dye altered the amount of DNA supercoiling in situ. The effect of ethidium bromide intercalation on incorporation of [3H]dTMP into acid-precipitable material in an in vitro DNA synthesis mixture was measured. The incorporation of [3H]dTMP was unaffected by less than 1 microgram/ml of ethidium bromide, enhanced up to two-fold by 1--10 microgram/ml, and inhibited by concentrations greater than 10 micrograms/ml. Alkaline sucrose gradient analysis revealed a higher percentage of small DNA fragments (6--20 S) in the cells treated with 2 micrograms/ml ethidium bromide than in control cells. These fragments attained parental size within the same time as the fragments in control cells. In cells treated with 2 micrograms/ml ethidium bromide, a significant fraction of newly synthesized DNA resulted from new starts, whereas in untreated cells practically none of the newly synthesized DNA resulted from new starts. These results suggest that relaxation of DNA supercoiled structures ahead of the replication fork generates spurious initiations of DNA synthesis and that in intact cells the rate of chain elongation is limited by supercoiled regions ahead of the growing point."}
{"id": "PMID:465492", "title": "Dependence of mammalian DNA replication on DNA supercoiling. II. Effects of novobiocin on DNA synthesis in Chinese hamster ovary cells.", "content": "Novobiocin, an inhibitor of gyrase-induced DNA supercoiling and DNA replication in prokaryotes, inhibited the incorporation of DNA precursors into DNA in both intact and permeable Chinese hamster ovary cells; much higher concentrations were required for permeable cells, in which no new replicons were initiated. Nucleoids were prepared from cells that were incubated for 60 min with 200 micrograms/ml novobiocin, made permeable, and incubated with 0--50 micrograms/ml ethidium bromide. Sedimentation of the nucleoids in neutral sucrose gradients suggested that the number of supercoils in the average nucleoid had been reduced by prior incubation with novobiocin. In intact cells, novobiocin is required inside the cell for continued inhibition of DNA synthesis, suggesting that it does not act directly on the DNA. Alkaline sucrose gradient profiles of DNA synthesized in the presence of novobiocin in intact cells indicated that the drug inhibited replicon initiation while having little if any effect on chain elongation. These data are consistent with the idea that an activity similar to the bacterial gyrase generates supercoils in mammalian DNA and produces the proper conformation for the initiation of DNA replication.", "contents": "Dependence of mammalian DNA replication on DNA supercoiling. II. Effects of novobiocin on DNA synthesis in Chinese hamster ovary cells. Novobiocin, an inhibitor of gyrase-induced DNA supercoiling and DNA replication in prokaryotes, inhibited the incorporation of DNA precursors into DNA in both intact and permeable Chinese hamster ovary cells; much higher concentrations were required for permeable cells, in which no new replicons were initiated. Nucleoids were prepared from cells that were incubated for 60 min with 200 micrograms/ml novobiocin, made permeable, and incubated with 0--50 micrograms/ml ethidium bromide. Sedimentation of the nucleoids in neutral sucrose gradients suggested that the number of supercoils in the average nucleoid had been reduced by prior incubation with novobiocin. In intact cells, novobiocin is required inside the cell for continued inhibition of DNA synthesis, suggesting that it does not act directly on the DNA. Alkaline sucrose gradient profiles of DNA synthesized in the presence of novobiocin in intact cells indicated that the drug inhibited replicon initiation while having little if any effect on chain elongation. These data are consistent with the idea that an activity similar to the bacterial gyrase generates supercoils in mammalian DNA and produces the proper conformation for the initiation of DNA replication."}
{"id": "PMID:465493", "title": "A rapid method for the purification of supercoiled PM2 DNA by affinity chromatography on H1 histone covalently coupled to agarose.", "content": "A simple and rapid method is described for the purification of supercoiled PM2 DNA by affinity chromatography on columns of H1 histone covalently coupled to agarose. The method does not require the use of intercalating agents or ultracentrifugation procedures. Under the conditions most appropriate for purification, elution is carried out in a single step with buffered 0.7 M NaCl after the sample has been loaded onto the column in buffered 0.2 M NaCl. The DNA eluted at the higher salt concentration consists of supercoiled closed circular DNA at greater than 90% purity independently of the ratio of supercoiled to nicked circular DNA in the input mixture.", "contents": "A rapid method for the purification of supercoiled PM2 DNA by affinity chromatography on H1 histone covalently coupled to agarose. A simple and rapid method is described for the purification of supercoiled PM2 DNA by affinity chromatography on columns of H1 histone covalently coupled to agarose. The method does not require the use of intercalating agents or ultracentrifugation procedures. Under the conditions most appropriate for purification, elution is carried out in a single step with buffered 0.7 M NaCl after the sample has been loaded onto the column in buffered 0.2 M NaCl. The DNA eluted at the higher salt concentration consists of supercoiled closed circular DNA at greater than 90% purity independently of the ratio of supercoiled to nicked circular DNA in the input mixture."}
{"id": "PMID:465494", "title": "DNA-binding antibiotics. X-ray structure of the distamycin A analog.", "content": "The crystal structure of the antibiotic distamycin A analog containing two pyrrol carboxamide fragments has been determined. The space group of the crystals is P21/b; the unit-cell dimensions are a = 11.169, b = 21.535, c = 7.863 A, alpha = beta = 90 degrees, gamma = 122.45 degrees, Z = 4. The structure is solved by direct methods, and refined with the full-matrix least-squares procedure. The data on the structure of the pyrrol carboxamide backbone allow the following conclusions to be made about the molecular structure of the distamycin type antibiotics: (i) the amide groups have normal trans-configuration with slightly shortened C-C and C-N bonds adjacent to the pyrrol rings, (ii) the N-methyl groups of the pyrrol rings and the oxygen atoms of the amide groups have the same orientation with respect to the backbone. In the distamycin A analog molecule the pyrrol rings and amide group between them are approximately coplanar.", "contents": "DNA-binding antibiotics. X-ray structure of the distamycin A analog. The crystal structure of the antibiotic distamycin A analog containing two pyrrol carboxamide fragments has been determined. The space group of the crystals is P21/b; the unit-cell dimensions are a = 11.169, b = 21.535, c = 7.863 A, alpha = beta = 90 degrees, gamma = 122.45 degrees, Z = 4. The structure is solved by direct methods, and refined with the full-matrix least-squares procedure. The data on the structure of the pyrrol carboxamide backbone allow the following conclusions to be made about the molecular structure of the distamycin type antibiotics: (i) the amide groups have normal trans-configuration with slightly shortened C-C and C-N bonds adjacent to the pyrrol rings, (ii) the N-methyl groups of the pyrrol rings and the oxygen atoms of the amide groups have the same orientation with respect to the backbone. In the distamycin A analog molecule the pyrrol rings and amide group between them are approximately coplanar."}
{"id": "PMID:465495", "title": "Actions of human DNA glycosylases on uracil-containing DNA, methylated DNA and their reconstituted chromatins.", "content": "Extracts of human lymphoblastoid cells catalyzed complete release of uracil (Ura) from PBS1 DNA, which contains Ura instead of thymine as a normal component (Ura-DNA), and 3-methyladenine (3-MeAde) from DNA methylated with methyl methanesulfonate (Me-DNA). These two activities, Ura-DNA glycosylase and 3-MeAde-DNA glycosylase, differed in heat stability. Cell extracts released Ura more rapidly and 3-MeAde more slowly from alkali-denatured preparations of Ura- and Me-DNA, respectively, than from native DNA's. On incubation with reconstituted chromatins, prepared from Ura-DNA and Me-DNA, respectively, with calf thymus chromosomal protein by salt gradient dialysis, cell extracts released all the Ura but only about half of the 3-MeAde residues, although both these chromatins were degraded by micrococcal nuclease until about half of the nucleotides became acid soluble. The activities of Ura-DNA and 3-MeAde-DNA glycosylase of xeroderma pigmentosum cells were similar to those of normal cells.", "contents": "Actions of human DNA glycosylases on uracil-containing DNA, methylated DNA and their reconstituted chromatins. Extracts of human lymphoblastoid cells catalyzed complete release of uracil (Ura) from PBS1 DNA, which contains Ura instead of thymine as a normal component (Ura-DNA), and 3-methyladenine (3-MeAde) from DNA methylated with methyl methanesulfonate (Me-DNA). These two activities, Ura-DNA glycosylase and 3-MeAde-DNA glycosylase, differed in heat stability. Cell extracts released Ura more rapidly and 3-MeAde more slowly from alkali-denatured preparations of Ura- and Me-DNA, respectively, than from native DNA's. On incubation with reconstituted chromatins, prepared from Ura-DNA and Me-DNA, respectively, with calf thymus chromosomal protein by salt gradient dialysis, cell extracts released all the Ura but only about half of the 3-MeAde residues, although both these chromatins were degraded by micrococcal nuclease until about half of the nucleotides became acid soluble. The activities of Ura-DNA and 3-MeAde-DNA glycosylase of xeroderma pigmentosum cells were similar to those of normal cells."}
{"id": "PMID:465496", "title": "Inhibition of DNA repair in ultraviolet-irradiated human cells by hydroxyurea.", "content": "The effect on DNA repair in ultraviolet-irradiated human skin fibroblasts by hydroxyurea has been examined in this study using three independent methods for measuring DNA repair:the 5-bromodeoxyuridine photolysis assay which measures DNA repair replication, chromatographic measurement of thymine-containing dimers, and measurement of specific ultraviolet-endonuclease-sensitive sites in irradiated DNA. Little effect of hydroxyurea was observed at the concentration of 2 mM, which is often used to inhibit semiconservative DNA synthesis; however, 10 mM hydroxyurea resulted in marked inhibition (65--70%) of excision repair. This inhibition was accompanied by a possible doubling in the size of the repaired region. The accumulation of large numbers of single-strand breaks following ultraviolet irradiation and hydroxyurea incubation seen by other investigators was not observed with the normal skin fibroblasts used in this study. A comparison of hydroxyurea effects on the different DNA repair assays indicates inhibition of one step in DNA repair also results in varying degrees of inhibition of other steps as well.", "contents": "Inhibition of DNA repair in ultraviolet-irradiated human cells by hydroxyurea. The effect on DNA repair in ultraviolet-irradiated human skin fibroblasts by hydroxyurea has been examined in this study using three independent methods for measuring DNA repair:the 5-bromodeoxyuridine photolysis assay which measures DNA repair replication, chromatographic measurement of thymine-containing dimers, and measurement of specific ultraviolet-endonuclease-sensitive sites in irradiated DNA. Little effect of hydroxyurea was observed at the concentration of 2 mM, which is often used to inhibit semiconservative DNA synthesis; however, 10 mM hydroxyurea resulted in marked inhibition (65--70%) of excision repair. This inhibition was accompanied by a possible doubling in the size of the repaired region. The accumulation of large numbers of single-strand breaks following ultraviolet irradiation and hydroxyurea incubation seen by other investigators was not observed with the normal skin fibroblasts used in this study. A comparison of hydroxyurea effects on the different DNA repair assays indicates inhibition of one step in DNA repair also results in varying degrees of inhibition of other steps as well."}
{"id": "PMID:465497", "title": "Rate-limiting factors for lymphocyte protein synthesis. Ribosome commitment and the capacity of lymphocyte cell-free systems to translate exogenous mRNAs.", "content": "The properties of the protein synthesising systems of different lymphocyte preparations have been compared with those of non-lymphoid tissues. Polysome profiles from rat thymocytes, sheep mesenteric lymphocytes, rat liver and mouse ascites tumours showed that the commitment of ribosomes to protein synthesis in lymphocytes was relatively low. Initiation factor activities, assessed on the abilities of post-mitochondrial fractions to support exogenous mRNA translation, were limited or undetectable in lymphoid tissues. While the thymocyte system translated globin mRNA, the response was enhanced by ascites extracts rich in initiation factors. The mesenteric lymphocyte system responded only marginally to globin mRNA and poly(U) but the responses were not enhanced by ascites extracts. The activity of isolated  mesenteric ribosomes was comparable with ribosomes from other tissues, indicating that extraribosomal factors were responsible for the poor overall activity of the mesenteric system. Finally, the effects of cycloheximide on the recruitment of polysomes in lymphocytes indicated that the commitment of ribosomes to protein synthesis might be restricted by both limited mRNA availability and limited capacity for initiation of mRNA translation.", "contents": "Rate-limiting factors for lymphocyte protein synthesis. Ribosome commitment and the capacity of lymphocyte cell-free systems to translate exogenous mRNAs. The properties of the protein synthesising systems of different lymphocyte preparations have been compared with those of non-lymphoid tissues. Polysome profiles from rat thymocytes, sheep mesenteric lymphocytes, rat liver and mouse ascites tumours showed that the commitment of ribosomes to protein synthesis in lymphocytes was relatively low. Initiation factor activities, assessed on the abilities of post-mitochondrial fractions to support exogenous mRNA translation, were limited or undetectable in lymphoid tissues. While the thymocyte system translated globin mRNA, the response was enhanced by ascites extracts rich in initiation factors. The mesenteric lymphocyte system responded only marginally to globin mRNA and poly(U) but the responses were not enhanced by ascites extracts. The activity of isolated  mesenteric ribosomes was comparable with ribosomes from other tissues, indicating that extraribosomal factors were responsible for the poor overall activity of the mesenteric system. Finally, the effects of cycloheximide on the recruitment of polysomes in lymphocytes indicated that the commitment of ribosomes to protein synthesis might be restricted by both limited mRNA availability and limited capacity for initiation of mRNA translation."}
{"id": "PMID:465498", "title": "Isolation of ribonuclease-free intact polyribosomes from rat kidney.", "content": "High levels of RNAase present in rat kidney have prevented isolation of intact polyribosomes from this tissue. This problem has been circumvented by a thorough in situ arterial perfusion of rat kidney, coupled with homogenization of the perfused rat kidney in heparin and detergents-fortified high-speed supernatant prepared from rat liver. This procedure reduced RNAase activity in the homogenate by as much as 70%. Sedimentation of the polyribosomes from this homogenate through a layer of 2.0 M sucrose resulted in a 78--80% yield of polyribosomes from the rat kidney. The resulting polyribosomal pellet contained less than 8% of the RNAase activity present in polyribosomes from non-perfused rat kidney. The remaining RNAase activity was separated from the larger polyribomes by sucrose density gradient centrifugation. The majority of the polyribosomes were larger than tetramers. This procedure also incrased both the yield and size of polyribosomes from rat and mouse liver.", "contents": "Isolation of ribonuclease-free intact polyribosomes from rat kidney. High levels of RNAase present in rat kidney have prevented isolation of intact polyribosomes from this tissue. This problem has been circumvented by a thorough in situ arterial perfusion of rat kidney, coupled with homogenization of the perfused rat kidney in heparin and detergents-fortified high-speed supernatant prepared from rat liver. This procedure reduced RNAase activity in the homogenate by as much as 70%. Sedimentation of the polyribosomes from this homogenate through a layer of 2.0 M sucrose resulted in a 78--80% yield of polyribosomes from the rat kidney. The resulting polyribosomal pellet contained less than 8% of the RNAase activity present in polyribosomes from non-perfused rat kidney. The remaining RNAase activity was separated from the larger polyribomes by sucrose density gradient centrifugation. The majority of the polyribosomes were larger than tetramers. This procedure also incrased both the yield and size of polyribosomes from rat and mouse liver."}
{"id": "PMID:465499", "title": "Peptidyl-puromycin synthesis by free and membrane-bound ribosomes.", "content": "The peptidyl transferase reaction, as measured by the formation of peptidyl-puromycin, was compared for free ribosomes and ribosomes bound to two types of membrane, the endoplasmic reticulum and the outer nuclear membrane. In most respects the reaction catalyzed by the three types of ribosome was similar, demonstrating that interaction of the 60 S ribosomal subunit with the membrane has little effect on the functioning of peptidyl transferase, a 60 S protein. However, both the rate and extent of synthesis of peptidyl puromycin were lower for ribosomes bound to the nuclear membrane than for free or microsome-bound ribosomes. This difference appears to be a direct consequence of the ribosome-membrane interaction, since ribosomes stripped from the nuclear membrane could not be distinguished from the other classes of ribosome.", "contents": "Peptidyl-puromycin synthesis by free and membrane-bound ribosomes. The peptidyl transferase reaction, as measured by the formation of peptidyl-puromycin, was compared for free ribosomes and ribosomes bound to two types of membrane, the endoplasmic reticulum and the outer nuclear membrane. In most respects the reaction catalyzed by the three types of ribosome was similar, demonstrating that interaction of the 60 S ribosomal subunit with the membrane has little effect on the functioning of peptidyl transferase, a 60 S protein. However, both the rate and extent of synthesis of peptidyl puromycin were lower for ribosomes bound to the nuclear membrane than for free or microsome-bound ribosomes. This difference appears to be a direct consequence of the ribosome-membrane interaction, since ribosomes stripped from the nuclear membrane could not be distinguished from the other classes of ribosome."}
{"id": "PMID:465500", "title": "Factors affecting the rate of purine ribonucleotide dephosphorylation in human erythrocytes.", "content": "Purine ribonucleotide dephosphorylation was measured in intact human erythrocytes in vitro to evaluate those factors which might regulate this process in vivo. It was found that purine nucleotides which exist predominantly in the triphosphate form (e.g. ATP and GTP) are protected from dephosphorylation while those nucleotides normally present as the monophosphate (e.g. IMP) are susceptible to dephosphorylation. This point was emphasised by studying an individual whose erythrocytes accumulated ITP rather than IMP; erythrocytes from this individual has a more stable pool of inosine phosphates than did erythrocytes from normal individuals. The concentration of intracellular phosphate was also shown to affect the rate of dephosphorylation. The dephosphorylation of IMP was inhibited at intracellular phosphate concentrations above approx. 3 mM. AMP dephosphorylation (in cells whose AMP concentration was increased by incubating them in the presence of 2-deoxyglucose) was inhibited by phosphate more strongly than was found for IMP. In contrast, the dephosphorylation of GMP did not appear to be affected by phosphate concentration. High oxygen tension was a powerful stimulator of IMP dephosphorylation while low oxygen tension protected IMP from dephosphorylation. This finding shows that human erythrocytes are similar to those of other mammals in this regard and points to a possible physiological determinant of purine turnover in these cells.", "contents": "Factors affecting the rate of purine ribonucleotide dephosphorylation in human erythrocytes. Purine ribonucleotide dephosphorylation was measured in intact human erythrocytes in vitro to evaluate those factors which might regulate this process in vivo. It was found that purine nucleotides which exist predominantly in the triphosphate form (e.g. ATP and GTP) are protected from dephosphorylation while those nucleotides normally present as the monophosphate (e.g. IMP) are susceptible to dephosphorylation. This point was emphasised by studying an individual whose erythrocytes accumulated ITP rather than IMP; erythrocytes from this individual has a more stable pool of inosine phosphates than did erythrocytes from normal individuals. The concentration of intracellular phosphate was also shown to affect the rate of dephosphorylation. The dephosphorylation of IMP was inhibited at intracellular phosphate concentrations above approx. 3 mM. AMP dephosphorylation (in cells whose AMP concentration was increased by incubating them in the presence of 2-deoxyglucose) was inhibited by phosphate more strongly than was found for IMP. In contrast, the dephosphorylation of GMP did not appear to be affected by phosphate concentration. High oxygen tension was a powerful stimulator of IMP dephosphorylation while low oxygen tension protected IMP from dephosphorylation. This finding shows that human erythrocytes are similar to those of other mammals in this regard and points to a possible physiological determinant of purine turnover in these cells."}
{"id": "PMID:465501", "title": "The cross-resistance of mouse blasticidin S-resistant cell lines to puromycin and sparsomycin, inhibitors of ribosome function.", "content": "The antibiotic blasticidin S inhibits peptide-chain elongation in extracts of bacteria and mammalian cells. After spontaneous or nitrosoguanidine mutagenesis, we have isolated 46 blasticidin S-resistant (Blar) cell lines independently from mouse mammary carcinoma cells (FM3a). Among those Blar clones, we studied two clones, a spontaneously induced one (S501) and a nitrosoguanidine mutagenized one (N742) in more detail. The resistant phenotype of these Blar cells is retained without change for at least four months in the absence of the antibiotic. These Blar cells are 10- to 20-fold more resistant to the cytotoxic action of the antibiotic than their parental cells in vivo. Polyuridylate dependent polyphenylalanine synthesis in vitro with S-30 extracts either from N742 or S501 is 10- to 50-fold more resistant to the inhibitory action of blasticidin S compared to the parental FM3a cells. Ribosomes from FM3a and N742 are fractionated into 40-S and 60-S subunits, and polyphenylalanine synthesis by mixing them in various combinations with S-100 fraction from mouse leukemia L5178Y cells indicating that the resistant phenotype of Blar cells is due to the alteration of 60 S ribosomal subunit. We also found that these two Blar cell lines (N742 and S501) show cross-resistance to gougerotin, puromycin and sparsomycin, but not to emetine or cycloheximide. The polyribosomal pattern of FM3a (Blas) and N742 was compared when the cells were incubated with 3 microgram/ml puromycin for 6 h. Puromycin treatment of Blas cells induced accumulation of monosomes and ribosomal subunits, while little if any transition of polyribosomes into monosome and ribosomal subunits appeared in its counterpart N742 treated with the same dose of puromycin.", "contents": "The cross-resistance of mouse blasticidin S-resistant cell lines to puromycin and sparsomycin, inhibitors of ribosome function. The antibiotic blasticidin S inhibits peptide-chain elongation in extracts of bacteria and mammalian cells. After spontaneous or nitrosoguanidine mutagenesis, we have isolated 46 blasticidin S-resistant (Blar) cell lines independently from mouse mammary carcinoma cells (FM3a). Among those Blar clones, we studied two clones, a spontaneously induced one (S501) and a nitrosoguanidine mutagenized one (N742) in more detail. The resistant phenotype of these Blar cells is retained without change for at least four months in the absence of the antibiotic. These Blar cells are 10- to 20-fold more resistant to the cytotoxic action of the antibiotic than their parental cells in vivo. Polyuridylate dependent polyphenylalanine synthesis in vitro with S-30 extracts either from N742 or S501 is 10- to 50-fold more resistant to the inhibitory action of blasticidin S compared to the parental FM3a cells. Ribosomes from FM3a and N742 are fractionated into 40-S and 60-S subunits, and polyphenylalanine synthesis by mixing them in various combinations with S-100 fraction from mouse leukemia L5178Y cells indicating that the resistant phenotype of Blar cells is due to the alteration of 60 S ribosomal subunit. We also found that these two Blar cell lines (N742 and S501) show cross-resistance to gougerotin, puromycin and sparsomycin, but not to emetine or cycloheximide. The polyribosomal pattern of FM3a (Blas) and N742 was compared when the cells were incubated with 3 microgram/ml puromycin for 6 h. Puromycin treatment of Blas cells induced accumulation of monosomes and ribosomal subunits, while little if any transition of polyribosomes into monosome and ribosomal subunits appeared in its counterpart N742 treated with the same dose of puromycin."}
{"id": "PMID:465502", "title": "Evidence for a single species of DNA ligase localized in nuclei of rat liver.", "content": "DNA ligase activity was distributed mainly in the extract of nuclei rapidly isolated from rat liver and about 20% of the nuclear activity in the cytoplasmic fraction. The DNA ligases of nuclear extract and cytoplasmic fraction showed similar gel-filtration patterns and the same sedimentation coefficient of 5.5 S. The appearance of 4-S enzyme from nuclei or nuclear extract was partly inhibited by phenylmethylsulfonyl fluoride. These results suggest that the major activity of DNA ligase in rat liver is due to a single species of the enzyme localized in nuclei and that 4-S nuclear DNA ligase reported previously is an artifact arising from 5.5-S enzyme during the isolation procedure.", "contents": "Evidence for a single species of DNA ligase localized in nuclei of rat liver. DNA ligase activity was distributed mainly in the extract of nuclei rapidly isolated from rat liver and about 20% of the nuclear activity in the cytoplasmic fraction. The DNA ligases of nuclear extract and cytoplasmic fraction showed similar gel-filtration patterns and the same sedimentation coefficient of 5.5 S. The appearance of 4-S enzyme from nuclei or nuclear extract was partly inhibited by phenylmethylsulfonyl fluoride. These results suggest that the major activity of DNA ligase in rat liver is due to a single species of the enzyme localized in nuclei and that 4-S nuclear DNA ligase reported previously is an artifact arising from 5.5-S enzyme during the isolation procedure."}
{"id": "PMID:465503", "title": "The crystal and molecular structure of 9-alpha-D-arabinofuranosyladenine.", "content": "The glycosyl torsional angles in two crystallographically-independent molecules of alpha-araA are -73 and -64 degrees, both of which are in the \"anti\" region. The sugar conformations are C(3')-endo and C(2')-exo-C(3')-endo.", "contents": "The crystal and molecular structure of 9-alpha-D-arabinofuranosyladenine. The glycosyl torsional angles in two crystallographically-independent molecules of alpha-araA are -73 and -64 degrees, both of which are in the \"anti\" region. The sugar conformations are C(3')-endo and C(2')-exo-C(3')-endo."}
{"id": "PMID:465504", "title": "Nucleoside phosphotransferase from yellow lupin seedling cotyledons.", "content": "Nucleoside phosphotransferase (nucleotide:3'-deoxynucleoside 5'-phosphotransferase, EC 2.7.1.77) from yellow lupin seedling cotyledons was purified and the active enzyme consists of a single polypeptide chain, Mr = 72 000 +/- 3000. In transphosphorylation, purine and pyrimidine nucleosides are good phosphate acceptors and 5'-nucleotides are effective phosphate donors. Among 2'- and 3'-nucleotides, only 3'-AMP and 3'-psi MP acted as phosphate donors, and p-nitrophenylphosphate appeared less active in this regard. The purine and pyrimidine bases inhibit transphosphorylation. The Km values determined for the inosine:5'-AMP pair were 400 micrometers for both the compounds. The enzyme showed optimum activity at pH 8.0 in mM Tris-HCl buffer. Antisulfhydryl reagents and EDTA did not affect enzyme activity.", "contents": "Nucleoside phosphotransferase from yellow lupin seedling cotyledons. Nucleoside phosphotransferase (nucleotide:3'-deoxynucleoside 5'-phosphotransferase, EC 2.7.1.77) from yellow lupin seedling cotyledons was purified and the active enzyme consists of a single polypeptide chain, Mr = 72 000 +/- 3000. In transphosphorylation, purine and pyrimidine nucleosides are good phosphate acceptors and 5'-nucleotides are effective phosphate donors. Among 2'- and 3'-nucleotides, only 3'-AMP and 3'-psi MP acted as phosphate donors, and p-nitrophenylphosphate appeared less active in this regard. The purine and pyrimidine bases inhibit transphosphorylation. The Km values determined for the inosine:5'-AMP pair were 400 micrometers for both the compounds. The enzyme showed optimum activity at pH 8.0 in mM Tris-HCl buffer. Antisulfhydryl reagents and EDTA did not affect enzyme activity."}
{"id": "PMID:465505", "title": "Aliphatic ketones are acetylcholinesterase inhibitors but not transition state analogs.", "content": "A number of C4--C9 aliphatic ketones are acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) inhibitors, with Ki values in the 0.7--5 mM range. Comparison to analogous substrates would suggest that these ketones are transition state analogs; e.g. 2-pentanone binds to the enzyme approx. 550 times more tightly than ethylacetate. However, a number of other criteria contradict this conclusions: (1) the binding is insensitive to ketone structure: isomeric ketones, cycloalkanones, and sterically hindered ketones have similar inhibitory potencies. (2) Analogous alcohols are also good inhibitors even though they cannot form hemiketals with the enzyme. (3) Representative ketones are relatively ineffective at blocking inactivation of the enzyme by methylsulfonyl fluoride, indicating that ketones do not bind principally at the hydrolytic site. (4) A competition experiment shows that binding of tetramethylammonium chloride excludes binding of 2-pentanone, suggesting that ketones bind to the anionic rather than the hydrolytic site. Thus, observation of tight binding relative to a substrate is not a sufficient criterion to establish that an inhibitor is a transition state analog.", "contents": "Aliphatic ketones are acetylcholinesterase inhibitors but not transition state analogs. A number of C4--C9 aliphatic ketones are acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) inhibitors, with Ki values in the 0.7--5 mM range. Comparison to analogous substrates would suggest that these ketones are transition state analogs; e.g. 2-pentanone binds to the enzyme approx. 550 times more tightly than ethylacetate. However, a number of other criteria contradict this conclusions: (1) the binding is insensitive to ketone structure: isomeric ketones, cycloalkanones, and sterically hindered ketones have similar inhibitory potencies. (2) Analogous alcohols are also good inhibitors even though they cannot form hemiketals with the enzyme. (3) Representative ketones are relatively ineffective at blocking inactivation of the enzyme by methylsulfonyl fluoride, indicating that ketones do not bind principally at the hydrolytic site. (4) A competition experiment shows that binding of tetramethylammonium chloride excludes binding of 2-pentanone, suggesting that ketones bind to the anionic rather than the hydrolytic site. Thus, observation of tight binding relative to a substrate is not a sufficient criterion to establish that an inhibitor is a transition state analog."}
{"id": "PMID:465506", "title": "The susceptibility of urokinase to affinity labeling by peptides of arginine chloromethyl ketone.", "content": "Pro-Gly-ArgCH2Cl, a reagent corresponding to the C-terminal sequence generated in plasminogen on activation by urokinase (EC 3.4.99.26) and probably by other plasminogen activators, was prepared. Pro-Gly-ArgCH2Cl was effective in the inactivation of urokinase at the 10(-6) M level (Ki 68 micrometers and k2 0.47 min-1). In contrast, only a slow inactivation was obtained by 10(-2) M N-tosyllysine chloromethyl ketone. Glu-Gly-ArgCH2Cl, N,N-dimethylaminonaphthalene-5-sulfonyl-Glu-Gly-ArgCH2Cl, and Ac-Gly-Gly-ArgCH2Cl were more reactive than Pro-Gly-ArgCH2Cl against urokinase by factors of 25, 6, and 3, respectively. The effectiveness of arginine chloromethyl ketones as affinity labels is highly dependent on binding in the S2 and S3 sites, thus sequence variations in the reagents exhibited differences in reactivity of up to four orders of magnitude. The most effective reagents had Gly in P2. Ac-Gly-Gly-ArgCH2Cl inactivates urokinase 50 times more rapidly than it does plasmin, thus providing a means of distinguishing the activity of plasmin from its activating protease whereas urokinase is almost inert to Ala-Phe-LysCH2Cl, a reagent which inactivates plasmin at the 10(-7) M level.", "contents": "The susceptibility of urokinase to affinity labeling by peptides of arginine chloromethyl ketone. Pro-Gly-ArgCH2Cl, a reagent corresponding to the C-terminal sequence generated in plasminogen on activation by urokinase (EC 3.4.99.26) and probably by other plasminogen activators, was prepared. Pro-Gly-ArgCH2Cl was effective in the inactivation of urokinase at the 10(-6) M level (Ki 68 micrometers and k2 0.47 min-1). In contrast, only a slow inactivation was obtained by 10(-2) M N-tosyllysine chloromethyl ketone. Glu-Gly-ArgCH2Cl, N,N-dimethylaminonaphthalene-5-sulfonyl-Glu-Gly-ArgCH2Cl, and Ac-Gly-Gly-ArgCH2Cl were more reactive than Pro-Gly-ArgCH2Cl against urokinase by factors of 25, 6, and 3, respectively. The effectiveness of arginine chloromethyl ketones as affinity labels is highly dependent on binding in the S2 and S3 sites, thus sequence variations in the reagents exhibited differences in reactivity of up to four orders of magnitude. The most effective reagents had Gly in P2. Ac-Gly-Gly-ArgCH2Cl inactivates urokinase 50 times more rapidly than it does plasmin, thus providing a means of distinguishing the activity of plasmin from its activating protease whereas urokinase is almost inert to Ala-Phe-LysCH2Cl, a reagent which inactivates plasmin at the 10(-7) M level."}
{"id": "PMID:465507", "title": "Chemical modification of histidyl and lysyl residues in yeast enolase.", "content": "Modification of yeast enolase (2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11) by diethyl pyrocarbonate at either pH 6.1 or 6.6 caused a biphasic inactivation of the enzyme. In the presence of excess Mg2+, either an equilibrium mixture of substrates or 3-phosphoglycerate, a competitive inhibitor, prevented the second slower phase of inactivation, but had no effect on the first rapid phase. Complete inactivation by diethyl pyrocarbonate correlates with the modification of six histidyl residues/subunit, while 3-phosphoglycerate protects two histidyl residues/subunit from modification. Modification of enolase by two lysine-specific reagents, 2,4,6-trinitrobenzenesulfonate and pyridoxal 5'-phosphate, at pH 8.3 caused a slow loss of enzyme activity. However, substrates did not significantly protect against inactivation by either reagent, and inactivation with 2,4,6-trinitrobenzenesulfonate correlates with the modification of 18 lysyl residues/enzyme subunit.", "contents": "Chemical modification of histidyl and lysyl residues in yeast enolase. Modification of yeast enolase (2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11) by diethyl pyrocarbonate at either pH 6.1 or 6.6 caused a biphasic inactivation of the enzyme. In the presence of excess Mg2+, either an equilibrium mixture of substrates or 3-phosphoglycerate, a competitive inhibitor, prevented the second slower phase of inactivation, but had no effect on the first rapid phase. Complete inactivation by diethyl pyrocarbonate correlates with the modification of six histidyl residues/subunit, while 3-phosphoglycerate protects two histidyl residues/subunit from modification. Modification of enolase by two lysine-specific reagents, 2,4,6-trinitrobenzenesulfonate and pyridoxal 5'-phosphate, at pH 8.3 caused a slow loss of enzyme activity. However, substrates did not significantly protect against inactivation by either reagent, and inactivation with 2,4,6-trinitrobenzenesulfonate correlates with the modification of 18 lysyl residues/enzyme subunit."}
{"id": "PMID:465508", "title": "Associative properties of butyryl-coenzyme A synthetase from ox liver mitochondria.", "content": "Butyryl-coenzyme A synthetase (butyrate:CoA ligase (AMP-forming), EC 6.2.1.2) from an acetone-dried powder of ox liver mitochondria was found to have a molecular weight of approx. 40 000. The sedimentation equilibrium analysis suggested the presence in solution of higher molecular weight forms of the enzyme and these could also be obtained by extracting the enzyme from the mitochondrial powder in non-reducing conditions. The enzyme was inhibited by sulphydryl reagents, and was found to have at least one available thiol group/molecule. The relationship between enzymic activity and concentration was non-linear, and suggested that an inactive monomer-active dimer equilibrium was present. The 5--6-fold activation by bovine serum albumin required the presence of free thiol groups in the albumin and involved association of albumin with the enzyme.", "contents": "Associative properties of butyryl-coenzyme A synthetase from ox liver mitochondria. Butyryl-coenzyme A synthetase (butyrate:CoA ligase (AMP-forming), EC 6.2.1.2) from an acetone-dried powder of ox liver mitochondria was found to have a molecular weight of approx. 40 000. The sedimentation equilibrium analysis suggested the presence in solution of higher molecular weight forms of the enzyme and these could also be obtained by extracting the enzyme from the mitochondrial powder in non-reducing conditions. The enzyme was inhibited by sulphydryl reagents, and was found to have at least one available thiol group/molecule. The relationship between enzymic activity and concentration was non-linear, and suggested that an inactive monomer-active dimer equilibrium was present. The 5--6-fold activation by bovine serum albumin required the presence of free thiol groups in the albumin and involved association of albumin with the enzyme."}
{"id": "PMID:465509", "title": "Subcellular localisation of di- and tripeptidases in guinea pig and rat enterocytes.", "content": "Enterocytes were isolated from rat and guinea pig jejunum and subcellular fractions were prepared by density gradient centrifugation. Gradient fractions were assayed for principal organelle marker enzymes and for di- and tripeptidases. The hydrolases showed a dual localisation with both brush border and cytosol components. In the rat, approximately equal portions of dipeptidase activities were found in the two fractions but, in the guinea pig, three times more activity were found in the two fractions but, in the guinea pig, three times more activity was found in the soluble than in the brush border fractions. Cytosol components in the rat were markedly inhibited by p-hydroxymercuribenzoate. In both species tripeptidase, leucyl-2-naphthylamidases and gamma-glutamyltransferase activities were found predominantly in the brush border fractions.", "contents": "Subcellular localisation of di- and tripeptidases in guinea pig and rat enterocytes. Enterocytes were isolated from rat and guinea pig jejunum and subcellular fractions were prepared by density gradient centrifugation. Gradient fractions were assayed for principal organelle marker enzymes and for di- and tripeptidases. The hydrolases showed a dual localisation with both brush border and cytosol components. In the rat, approximately equal portions of dipeptidase activities were found in the two fractions but, in the guinea pig, three times more activity were found in the two fractions but, in the guinea pig, three times more activity was found in the soluble than in the brush border fractions. Cytosol components in the rat were markedly inhibited by p-hydroxymercuribenzoate. In both species tripeptidase, leucyl-2-naphthylamidases and gamma-glutamyltransferase activities were found predominantly in the brush border fractions."}
{"id": "PMID:465510", "title": "Studies on the formation of N6-hydroxylysine in cell-free extracts of Aerobacter aerogenes 62-1.", "content": "We have investigated conditions optimal for the conversion of L-lysine to its N6-hydroxy derivative by partially purified cell-free extracts of Aerobacter aerogenes 62-1. The enzyme system was highly specific to L-lysine: the D-isomer and, the N2- or N6-derivatives of lysine, and alpha-amino acids were not hydroxylated. Most of the latter compounds had little effect onthe hydroxylation of L-lysine. However, -l-glutamic acid and L-glutamine enhanced the hydroxylation, with half-maximal activation achieved at 100 micrometers concentration of the effector. The Km values for pyruvate and L-(+)-lactate (compounds known to stimulate N-hydroxylysine formation) were found to be approx. 100 micrometers. The data show that N-hydroxylation of the amino acid precedes acylation in the biosynthesis of hydroxamic acid in A. aerogenes 62-1.", "contents": "Studies on the formation of N6-hydroxylysine in cell-free extracts of Aerobacter aerogenes 62-1. We have investigated conditions optimal for the conversion of L-lysine to its N6-hydroxy derivative by partially purified cell-free extracts of Aerobacter aerogenes 62-1. The enzyme system was highly specific to L-lysine: the D-isomer and, the N2- or N6-derivatives of lysine, and alpha-amino acids were not hydroxylated. Most of the latter compounds had little effect onthe hydroxylation of L-lysine. However, -l-glutamic acid and L-glutamine enhanced the hydroxylation, with half-maximal activation achieved at 100 micrometers concentration of the effector. The Km values for pyruvate and L-(+)-lactate (compounds known to stimulate N-hydroxylysine formation) were found to be approx. 100 micrometers. The data show that N-hydroxylation of the amino acid precedes acylation in the biosynthesis of hydroxamic acid in A. aerogenes 62-1."}
{"id": "PMID:465511", "title": "The stimulation of erucate metabolism in isolated rat hepatocytes by rapeseed oil and hydrogenated marine oil-containing diets.", "content": "1. The metabolism of palmitate and especially of erucate was studied in hepatocytes isolated from rats fed for 3 weeks a diet containing peanut oil (diet, 1), rapeseed oil (diet 2) and partially hydrogenated marine oil (diet 3). 2. The metabolism of palmitate was not significantly influenced by the diet. The rapeseed oil diet caused 1.4 fold and 1.3 fold increase and marine oil diet 3 fold and 2.2 fold increase in the oxidation and chain-shortening respectively of [14-14C]erucic acid in isolated hepatocytes. 3. Cyanide and antimycin A did not inhibit the chain-shortening of erucate in liver cells of rats fed rapeseed oil and peanut oil. The high capacity of the chain-shortening system in hepatocytes of marine oil-fed rats was partially inhibited. 4. Inhibition of the transfer of fatty acids into the mitochondria by lowering the intracellular carnitine concentration and/or by addition of (+)-decanoyl-carnitine resulted in a very pronounced apparent stimulation of the chain-shortening of erucic acid. It is suggested that the chain-shortening system may be virtually independent of the mitochondria, unless the availability of the extramitochondria NAD+ and/or NADP+ is rate-limiting under conditions of extremely low redox potential of the mitochondria. 5. Feeding marine oil or rapeseed oil to the rats induced a 30% increase in catalase activity, a 25--30% increase in urate oxidase activity and a 50% increase in the total CoA in the liver compared to rats fed peanut oil. 6. It is suggested that the increased metabolism of erucate in hepatocytes of marine oil and rapeseed oil-fed rats may be due to the increase in ther peroxisomal beta-oxidation.", "contents": "The stimulation of erucate metabolism in isolated rat hepatocytes by rapeseed oil and hydrogenated marine oil-containing diets. 1. The metabolism of palmitate and especially of erucate was studied in hepatocytes isolated from rats fed for 3 weeks a diet containing peanut oil (diet, 1), rapeseed oil (diet 2) and partially hydrogenated marine oil (diet 3). 2. The metabolism of palmitate was not significantly influenced by the diet. The rapeseed oil diet caused 1.4 fold and 1.3 fold increase and marine oil diet 3 fold and 2.2 fold increase in the oxidation and chain-shortening respectively of [14-14C]erucic acid in isolated hepatocytes. 3. Cyanide and antimycin A did not inhibit the chain-shortening of erucate in liver cells of rats fed rapeseed oil and peanut oil. The high capacity of the chain-shortening system in hepatocytes of marine oil-fed rats was partially inhibited. 4. Inhibition of the transfer of fatty acids into the mitochondria by lowering the intracellular carnitine concentration and/or by addition of (+)-decanoyl-carnitine resulted in a very pronounced apparent stimulation of the chain-shortening of erucic acid. It is suggested that the chain-shortening system may be virtually independent of the mitochondria, unless the availability of the extramitochondria NAD+ and/or NADP+ is rate-limiting under conditions of extremely low redox potential of the mitochondria. 5. Feeding marine oil or rapeseed oil to the rats induced a 30% increase in catalase activity, a 25--30% increase in urate oxidase activity and a 50% increase in the total CoA in the liver compared to rats fed peanut oil. 6. It is suggested that the increased metabolism of erucate in hepatocytes of marine oil and rapeseed oil-fed rats may be due to the increase in ther peroxisomal beta-oxidation."}
{"id": "PMID:465512", "title": "Formation of cholic acid via 3 alpha, 7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid in the dog.", "content": "The proposed cholic precursor, 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-[3H]cholestan-26-oic acid, and [14C]cholesterol were infused intravenously at a constant rate into two dogs for 25 days. If the specific activities of trihydroxy[3H]cholestanoic acid and [3H]cholic acid will be equal after an isotopic steady-state is achieved. The specific activities of [14C]deoxycholic acid (formed from [14C]cholic acid) isolated in the stool of these two dogs were equal the last four days of the infusion indicating that labeled deoxycholic acid (and presumably labeled cholic acid) was in an isotopic steady-state. However, the specific activities of trihydroxy[3H]cholestanoic acid were 3.3 and 5.7 times greater than the specific activities of [3H]cholic acid, respectively. These data suggest that either an alternate route of cholic acid synthesis exists exclusive of trihydroxycholestanoic acid or that an isotopic steady state of trihydroxycholestanoic acid cannot be reached during an infusion of labeled trihydroxycholestanoic acid.", "contents": "Formation of cholic acid via 3 alpha, 7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid in the dog. The proposed cholic precursor, 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-[3H]cholestan-26-oic acid, and [14C]cholesterol were infused intravenously at a constant rate into two dogs for 25 days. If the specific activities of trihydroxy[3H]cholestanoic acid and [3H]cholic acid will be equal after an isotopic steady-state is achieved. The specific activities of [14C]deoxycholic acid (formed from [14C]cholic acid) isolated in the stool of these two dogs were equal the last four days of the infusion indicating that labeled deoxycholic acid (and presumably labeled cholic acid) was in an isotopic steady-state. However, the specific activities of trihydroxy[3H]cholestanoic acid were 3.3 and 5.7 times greater than the specific activities of [3H]cholic acid, respectively. These data suggest that either an alternate route of cholic acid synthesis exists exclusive of trihydroxycholestanoic acid or that an isotopic steady state of trihydroxycholestanoic acid cannot be reached during an infusion of labeled trihydroxycholestanoic acid."}
{"id": "PMID:465513", "title": "Selective measurement of triacylglycerol lipase activities in pig post-heparin plasma.", "content": "Immunochemical methods for the selective measurement of pig post-heparin plasma lipoprotein lipase and hepatic lipase are described and validated. A simple two step purification method for porcine hepatic lipase from hepatic perfusate based on affinity chromatography and gel filtration is reported. The activity of the post-heparin plasma lipoprotein lipase and hepatic lipase in swine is reported. It is demonstrated that fasting decreases the activity of post-heparin plasma lipoprotein lipase activity more than two-fold while it does not affect the hepatic lipase activity significantly.", "contents": "Selective measurement of triacylglycerol lipase activities in pig post-heparin plasma. Immunochemical methods for the selective measurement of pig post-heparin plasma lipoprotein lipase and hepatic lipase are described and validated. A simple two step purification method for porcine hepatic lipase from hepatic perfusate based on affinity chromatography and gel filtration is reported. The activity of the post-heparin plasma lipoprotein lipase and hepatic lipase in swine is reported. It is demonstrated that fasting decreases the activity of post-heparin plasma lipoprotein lipase activity more than two-fold while it does not affect the hepatic lipase activity significantly."}
{"id": "PMID:465514", "title": "Lipid biosynthesis in the marine flatworm Convoluta roscoffensis and its algal symbiont Platymonas convoluta.", "content": "As a part of an investigations on the lipid metabolism in Platyhelminthes, the acoel Convoluta roscoffensis, which harbors the green alga Platymonas convoluta as a symbiont, was studied. Isotopic tracer experiments established that the acoel lacks the ability to synthesize de novo long-chain saturated and unsaturated fatty acids and depends on its algal symbiont for these compounds. The acoel's fatty acid composition closely resembles that of the alga but differs from those of other animals; the acoel's polyunsaturated fatty acids are of the plant type (omega 3 family) rather than of the animal type (omega 6 family). The acoel also lacks the ability to synthesize sterols de novo. It contains 24-methylenecholesterol synthesized by the algae and, in addition, significant amounts of cholesterol, which is probably a host modification product of the algal sterol. With fatty acids provided by the symbiont, the acoel has the ability to synthesize its own complex lipids. The acoel contains relatively large amounts of triglyceride, phosphatidylcholine, and ethanolamine plasmalogen. These compounds are either not present at all or present only in minute amounts in the symbiotic alga. Since acoels belong to the most primitive forms of the present-day flatworms, the observed metabolic defects in this organism suggest that mechanisms for the biosynthesis of fatty acids and sterols were lost early during the evolution of the Platyhelminthes, and that this phenomenon is widespread within the phylum.", "contents": "Lipid biosynthesis in the marine flatworm Convoluta roscoffensis and its algal symbiont Platymonas convoluta. As a part of an investigations on the lipid metabolism in Platyhelminthes, the acoel Convoluta roscoffensis, which harbors the green alga Platymonas convoluta as a symbiont, was studied. Isotopic tracer experiments established that the acoel lacks the ability to synthesize de novo long-chain saturated and unsaturated fatty acids and depends on its algal symbiont for these compounds. The acoel's fatty acid composition closely resembles that of the alga but differs from those of other animals; the acoel's polyunsaturated fatty acids are of the plant type (omega 3 family) rather than of the animal type (omega 6 family). The acoel also lacks the ability to synthesize sterols de novo. It contains 24-methylenecholesterol synthesized by the algae and, in addition, significant amounts of cholesterol, which is probably a host modification product of the algal sterol. With fatty acids provided by the symbiont, the acoel has the ability to synthesize its own complex lipids. The acoel contains relatively large amounts of triglyceride, phosphatidylcholine, and ethanolamine plasmalogen. These compounds are either not present at all or present only in minute amounts in the symbiotic alga. Since acoels belong to the most primitive forms of the present-day flatworms, the observed metabolic defects in this organism suggest that mechanisms for the biosynthesis of fatty acids and sterols were lost early during the evolution of the Platyhelminthes, and that this phenomenon is widespread within the phylum."}
{"id": "PMID:465515", "title": "Effect of sphingosine and other amphiphilic amines on the biosynthesis of phosphatidylethanolamine and other glycerolipids in isolated rat hepatocytes.", "content": "The importance of ethanolamine and sphingosine as precursors of phosphoethanolamine was investigated by incubating them with [3H]glycerol and isolated rat hepatocytes. Sphingosine (0.1--0.5 mM) stimulated the synthesis of phosphatidylethanolamine from [3H]glycerol, but the stimulation by ethanolamine was more pronounced. Furthermore, more phosphoethanolamine accumulated in the heptatocytes after incubation with ethanolamine than after incubation with sphingosine. It is concluded that ethanolamine is the most important phosphoethanolamine precursor in rat liver. Higher concentrations of sphingosine caused accumulation of [3H]phosphatidate and inhibition of total glycerolipid synthesis in isolated hepatocytes, when incubated in the presence of [3H]glycerol. These effects were very similar to those of fenfluramine and norfenfluramine described previously. Simpler cationic amphiphilic amines, like oleoylamine and octadecyltrimethylammonium bromide, also caused these effects. Variation of alkyl chain length and amphiphile charge showed that both a positive charge and a certain alkyl chain length were necessary for interference with phosphatidate metabolism. A much wider range of compounds inhibited total glycerolipid synthesis from [3H]glycerol.", "contents": "Effect of sphingosine and other amphiphilic amines on the biosynthesis of phosphatidylethanolamine and other glycerolipids in isolated rat hepatocytes. The importance of ethanolamine and sphingosine as precursors of phosphoethanolamine was investigated by incubating them with [3H]glycerol and isolated rat hepatocytes. Sphingosine (0.1--0.5 mM) stimulated the synthesis of phosphatidylethanolamine from [3H]glycerol, but the stimulation by ethanolamine was more pronounced. Furthermore, more phosphoethanolamine accumulated in the heptatocytes after incubation with ethanolamine than after incubation with sphingosine. It is concluded that ethanolamine is the most important phosphoethanolamine precursor in rat liver. Higher concentrations of sphingosine caused accumulation of [3H]phosphatidate and inhibition of total glycerolipid synthesis in isolated hepatocytes, when incubated in the presence of [3H]glycerol. These effects were very similar to those of fenfluramine and norfenfluramine described previously. Simpler cationic amphiphilic amines, like oleoylamine and octadecyltrimethylammonium bromide, also caused these effects. Variation of alkyl chain length and amphiphile charge showed that both a positive charge and a certain alkyl chain length were necessary for interference with phosphatidate metabolism. A much wider range of compounds inhibited total glycerolipid synthesis from [3H]glycerol."}
{"id": "PMID:465516", "title": "Mixed micelles of sphingomyelin and phosphatidylcholine with nonionic surfactants. Effect of temperature and surfactant polydispersity.", "content": "Mixed micelle formation of the polydisperse nonionic surfactant Triton X-100 as well as its homogeneous analogue, p-(1,1,3,3-tetramethylbutyl)-phenoxynonaoxyethylene glycol (OPE-9), with bovine brain sphingomyelin or dipalmitoyl phosphatidylcholine has been characterized by column chromatography on 6% agarose. At 40 degrees C, mixtures of OPE-9 and either sphingomyelin or dipalmitoyl phosphatidylcholine give a narrow size distribution for mixed micelles. A this temperature the size distribution of Triton X-100-containing mixed micelles is complicated because of the polydispersity of the oxyethylene chains. At 20 degrees C narrow size distributions are observed for mixed micelles of sphingomyelin/Triton X-100 and sphingomyelin/OPE-9 up to at least 0.06 mol fraction of lipid. For dipalmitoyl phosphatidylcholine this is observed only with OPE-9. At intermediate mol fractions of lipid (around 0.25), two populations of mixed micelles exist for sphingomyelin/Trition X-100, sphingomyelin/OPE-9, and dipalmitoyl phosphatidylcholine/OPE-9. At high mol fractions of lipid only one population of mixed micelles again exists. At 20 degrees C, sphingoymelin forms a clear solution with Triton X-100 and OPE-9 to a lipid mol fraction of at least 0.46 and 0.67, respectively. Dipalmitoyl phosphatidylcholine forms a clear solution with OPE-9 to a lipid mol fraction of at least 0.57 at the same temperature. Triton X-100 and dipalmitoyl phosphatidylcholine do not form stable, clear solutions at 20 degrees C unless the lipid mol fraction is extremely low. These results show that surfactant polydispersity and temperature are important determinants in the solubilization of lipids by nonionic surfactants. It is also shown that pure surfactant micelles and lipid/surfactant mixed micelles do not co-exist in the same solution.", "contents": "Mixed micelles of sphingomyelin and phosphatidylcholine with nonionic surfactants. Effect of temperature and surfactant polydispersity. Mixed micelle formation of the polydisperse nonionic surfactant Triton X-100 as well as its homogeneous analogue, p-(1,1,3,3-tetramethylbutyl)-phenoxynonaoxyethylene glycol (OPE-9), with bovine brain sphingomyelin or dipalmitoyl phosphatidylcholine has been characterized by column chromatography on 6% agarose. At 40 degrees C, mixtures of OPE-9 and either sphingomyelin or dipalmitoyl phosphatidylcholine give a narrow size distribution for mixed micelles. A this temperature the size distribution of Triton X-100-containing mixed micelles is complicated because of the polydispersity of the oxyethylene chains. At 20 degrees C narrow size distributions are observed for mixed micelles of sphingomyelin/Triton X-100 and sphingomyelin/OPE-9 up to at least 0.06 mol fraction of lipid. For dipalmitoyl phosphatidylcholine this is observed only with OPE-9. At intermediate mol fractions of lipid (around 0.25), two populations of mixed micelles exist for sphingomyelin/Trition X-100, sphingomyelin/OPE-9, and dipalmitoyl phosphatidylcholine/OPE-9. At high mol fractions of lipid only one population of mixed micelles again exists. At 20 degrees C, sphingoymelin forms a clear solution with Triton X-100 and OPE-9 to a lipid mol fraction of at least 0.46 and 0.67, respectively. Dipalmitoyl phosphatidylcholine forms a clear solution with OPE-9 to a lipid mol fraction of at least 0.57 at the same temperature. Triton X-100 and dipalmitoyl phosphatidylcholine do not form stable, clear solutions at 20 degrees C unless the lipid mol fraction is extremely low. These results show that surfactant polydispersity and temperature are important determinants in the solubilization of lipids by nonionic surfactants. It is also shown that pure surfactant micelles and lipid/surfactant mixed micelles do not co-exist in the same solution."}
{"id": "PMID:465517", "title": "Activation of lipoprotein lipase by native and acylated peptides of apolipoprotein C-II.", "content": "Apolipoprotein C-II, a protein found associated with all major classes of plasma lipoproteins, is a potent activator of the enzyme lipoprotein lipase. We have prepared the maleyl, citraconyl and succinyl derivatives of apolipoprotein C-II, and compared the capacities of the intact and tryptically cleaved proteins to activate lipoprotein lipase. The NH2-terminal 50 residue peptide proved virtually inactive, even after removal of the masking groups from the citraconyl derivative. The COOH-terminal 29 residue peptides of maleyl and citraconyl apolipoprotein C-II were more active than the corresponding succinylated peptide. After deacylation of the citraconyl derivative, the COOH-terminal peptide had maximal activity as great as apolipoprotein C-II, although the profile of activation remained dissimilar at low activator concentrations.", "contents": "Activation of lipoprotein lipase by native and acylated peptides of apolipoprotein C-II. Apolipoprotein C-II, a protein found associated with all major classes of plasma lipoproteins, is a potent activator of the enzyme lipoprotein lipase. We have prepared the maleyl, citraconyl and succinyl derivatives of apolipoprotein C-II, and compared the capacities of the intact and tryptically cleaved proteins to activate lipoprotein lipase. The NH2-terminal 50 residue peptide proved virtually inactive, even after removal of the masking groups from the citraconyl derivative. The COOH-terminal 29 residue peptides of maleyl and citraconyl apolipoprotein C-II were more active than the corresponding succinylated peptide. After deacylation of the citraconyl derivative, the COOH-terminal peptide had maximal activity as great as apolipoprotein C-II, although the profile of activation remained dissimilar at low activator concentrations."}
{"id": "PMID:465518", "title": "Partitioning of bile acids into subcellular organelles and the in vivo distribution of bile acids in rat liver.", "content": "1. The subcellular distribution of conjugates of cholic acid and chenodeoxycholic acid between cytosol, nuclei, mitochondria and microsomes in rat liver has been determined. 2. The partition coefficients for the distribution of these bile acids between subcellular fractions and buffer have been measured and used to construct a compartmental model of the amounts of conjugated bile acids present in the different subcellular organelles in vivo. 3. This model indicates that a large percentage of the bile acid in the rat liver is found in the nuclear fraction; 42% of the cholic acid conjugates and 27% of the chenodeoxycholic acid conjugates. Substantial amounts of bile acid are also present in microsomes and mitochondria suggesting that published estimates of the amounts of bile acids in these fractions are underestimates. 4. The model also allows the amount of bile acid which is in free solution in cytosol to be determined; 10.9% of the cholic acid conjugates and 4.1% of the chenodeoxycholic acid conjugates in rat liver were present in this fraction. Knowlege of the amount of free bile acid allows possible roles of the cytosolic bile binding proteins to be assessed.", "contents": "Partitioning of bile acids into subcellular organelles and the in vivo distribution of bile acids in rat liver. 1. The subcellular distribution of conjugates of cholic acid and chenodeoxycholic acid between cytosol, nuclei, mitochondria and microsomes in rat liver has been determined. 2. The partition coefficients for the distribution of these bile acids between subcellular fractions and buffer have been measured and used to construct a compartmental model of the amounts of conjugated bile acids present in the different subcellular organelles in vivo. 3. This model indicates that a large percentage of the bile acid in the rat liver is found in the nuclear fraction; 42% of the cholic acid conjugates and 27% of the chenodeoxycholic acid conjugates. Substantial amounts of bile acid are also present in microsomes and mitochondria suggesting that published estimates of the amounts of bile acids in these fractions are underestimates. 4. The model also allows the amount of bile acid which is in free solution in cytosol to be determined; 10.9% of the cholic acid conjugates and 4.1% of the chenodeoxycholic acid conjugates in rat liver were present in this fraction. Knowlege of the amount of free bile acid allows possible roles of the cytosolic bile binding proteins to be assessed."}
{"id": "PMID:465520", "title": "Studies on the biosynthesis of sulfolipids in the Diatom Nitzschia alba.", "content": "Labeling of sulfolipids in Nitzschia alba was studied after growth of the cells in media containing L-[35S]cystine, L-[35S], L-[35S]cysteine, L-[35S]-methionine or a mixture of L-[Me-3H]methionine and L-[35S]methionine, [35S]Cysteine or [35S]cystine labeled the deoxyceramide sulfonate and the sulfonium analog, phosphatidylsulfocholine (and its lyso derivative) but not the sterol sulfate nor the sulfoquinovosyl diglyceride; [35S]methionine labeled only the phosphatidylsulfocholine and its lyso derivative. With the [35S]- and [Me-3H]methionine mixture (3H/35S ratio 1.0) the phosphatidylsulfocholine had a 3H/35 S ratio of 1.5 indicating that both sulfonium methyl groups were derived from methionine. Probable biosynthetic pathways for these novel sulfolipids are discussed.", "contents": "Studies on the biosynthesis of sulfolipids in the Diatom Nitzschia alba. Labeling of sulfolipids in Nitzschia alba was studied after growth of the cells in media containing L-[35S]cystine, L-[35S], L-[35S]cysteine, L-[35S]-methionine or a mixture of L-[Me-3H]methionine and L-[35S]methionine, [35S]Cysteine or [35S]cystine labeled the deoxyceramide sulfonate and the sulfonium analog, phosphatidylsulfocholine (and its lyso derivative) but not the sterol sulfate nor the sulfoquinovosyl diglyceride; [35S]methionine labeled only the phosphatidylsulfocholine and its lyso derivative. With the [35S]- and [Me-3H]methionine mixture (3H/35S ratio 1.0) the phosphatidylsulfocholine had a 3H/35 S ratio of 1.5 indicating that both sulfonium methyl groups were derived from methionine. Probable biosynthetic pathways for these novel sulfolipids are discussed."}
{"id": "PMID:465521", "title": "Disulfide-linked peptides in the chloroplast thylakoid membrane.", "content": "Two peptides move out from the diagonal when dodecyl sulfate gels of pea leaf chloroplast thylakoid membrane proteins are treated with a reducing agent and electrophoresed in the second dimension. Apparently two of the proteins in the thylakoid membrane are disulfide linked dimers. Additional changes in the electrophoretic mobility of certian peptides occur when thylakoid membranes are denatured in the presence of reducing agents: these peptides probably contain intrapeptide disulfide bonds or prosthetic groups covalently attached to the peptide through disulfide bonds.", "contents": "Disulfide-linked peptides in the chloroplast thylakoid membrane. Two peptides move out from the diagonal when dodecyl sulfate gels of pea leaf chloroplast thylakoid membrane proteins are treated with a reducing agent and electrophoresed in the second dimension. Apparently two of the proteins in the thylakoid membrane are disulfide linked dimers. Additional changes in the electrophoretic mobility of certian peptides occur when thylakoid membranes are denatured in the presence of reducing agents: these peptides probably contain intrapeptide disulfide bonds or prosthetic groups covalently attached to the peptide through disulfide bonds."}
{"id": "PMID:465522", "title": "Disulfide interaction in situ between two neighbouring proteins in mammalian 60-S ribosomal subunits. Isolation of the contact region of the larger protein.", "content": "A disulfide complex is formed in situ under gentle conditions between two neighbouring proteins in the 60-S subunits of mammalian ribosomes. The proteins have been identified as L 4 and L 29. The complex is easily isolated from whole ribosomes, and can be utilized for preparing the two proteins in a very pure state for further characterization. Chymotryptic cleavage of the complex or the isolated larger protein (L 4) in the presence of SDS produces two unequal fragments of this protein in nearly quantitative yield. The smaller fragment (approx. 12 000 daltons) contains the contact sequence. Only this fragment of protein L 4 is labelled when rat liver ribosomes are incuabted with iodo[14C]acetate under conditions of complex formation. Protein L 29 is resistant to chymotrypsin in the presence of sodium dodecyl sulfate.", "contents": "Disulfide interaction in situ between two neighbouring proteins in mammalian 60-S ribosomal subunits. Isolation of the contact region of the larger protein. A disulfide complex is formed in situ under gentle conditions between two neighbouring proteins in the 60-S subunits of mammalian ribosomes. The proteins have been identified as L 4 and L 29. The complex is easily isolated from whole ribosomes, and can be utilized for preparing the two proteins in a very pure state for further characterization. Chymotryptic cleavage of the complex or the isolated larger protein (L 4) in the presence of SDS produces two unequal fragments of this protein in nearly quantitative yield. The smaller fragment (approx. 12 000 daltons) contains the contact sequence. Only this fragment of protein L 4 is labelled when rat liver ribosomes are incuabted with iodo[14C]acetate under conditions of complex formation. Protein L 29 is resistant to chymotrypsin in the presence of sodium dodecyl sulfate."}
{"id": "PMID:465523", "title": "A theoretical interpretation of the variations of some physical parameters within the [2Fe-2S] ferredoxin group.", "content": "A model is proposed to explain the variation of some physical parameters within the reduced [2Fe-2S] ferredoxin group. According to this model, the main effects result from a variable mixing of some d orbitals of the Fe2+ ion owing to rhombic distortion of the active site having the same geometrical character, but different in intensity, for each protein. Some peculiar experimental results such as the axial electron paramagnetic resonance spectra of adrenal ferredoxin and Pseudomonas putida ferredoxin and the electric field gradient tensor of P. putida ferredoxin are explained without assuming properties drastically different from those of the other ferredoxins, as had been suggested in the literature.", "contents": "A theoretical interpretation of the variations of some physical parameters within the [2Fe-2S] ferredoxin group. A model is proposed to explain the variation of some physical parameters within the reduced [2Fe-2S] ferredoxin group. According to this model, the main effects result from a variable mixing of some d orbitals of the Fe2+ ion owing to rhombic distortion of the active site having the same geometrical character, but different in intensity, for each protein. Some peculiar experimental results such as the axial electron paramagnetic resonance spectra of adrenal ferredoxin and Pseudomonas putida ferredoxin and the electric field gradient tensor of P. putida ferredoxin are explained without assuming properties drastically different from those of the other ferredoxins, as had been suggested in the literature."}
{"id": "PMID:465524", "title": "Circular dichroism spectra of purified cytochromes P-450 from rabbit liver microsomes.", "content": "Circular dichroism (CD) spectra were measured for cytochromes P-450 (P-450) purified from phenobarbital- and 3-methylcholanthrene-induced rabbit liver microsomes. No striking difference in alpha-helix content was seen between phenobarbital-induced P-450 (PB P-450) (50%), phenobarbital-induced P-448 (PB P-448) (40%) and 3-methylcholanthrene-induced P-448 (MC P-448) (45--50%) in terms of ultraviolet CD spectra. Strong negative CD spectra associated with 3-methylcholanthrene transitions for MC P-448 in the near-ultraviolet region (250--310 nm) and weaker negative CD spectra associated with Soret transitions for PBP-448 ([theta] = 50 000) and MCP-448 ([theta] = 160 000), indicated that structures of these preparations are strikingly different from each other. Reduction of P-450 and P-448 led to a remarkable decrease of the Soret CD trough, suggesting that reduction was accompanied by a striking conformational change in the vicinity of the heme. Since CO complexes of reduced P-450 and P-448 showed a CD trough and an S-shaped CD, respectively, associated with the absorption peak at 450 nm, the heme vicinities are remarkably different from each other. The CD spectra in the visible region are also discussed. It was noticed that P-420, the denatured form of P-450, exhibited no CD spectra in the Soret and visible regions.", "contents": "Circular dichroism spectra of purified cytochromes P-450 from rabbit liver microsomes. Circular dichroism (CD) spectra were measured for cytochromes P-450 (P-450) purified from phenobarbital- and 3-methylcholanthrene-induced rabbit liver microsomes. No striking difference in alpha-helix content was seen between phenobarbital-induced P-450 (PB P-450) (50%), phenobarbital-induced P-448 (PB P-448) (40%) and 3-methylcholanthrene-induced P-448 (MC P-448) (45--50%) in terms of ultraviolet CD spectra. Strong negative CD spectra associated with 3-methylcholanthrene transitions for MC P-448 in the near-ultraviolet region (250--310 nm) and weaker negative CD spectra associated with Soret transitions for PBP-448 ([theta] = 50 000) and MCP-448 ([theta] = 160 000), indicated that structures of these preparations are strikingly different from each other. Reduction of P-450 and P-448 led to a remarkable decrease of the Soret CD trough, suggesting that reduction was accompanied by a striking conformational change in the vicinity of the heme. Since CO complexes of reduced P-450 and P-448 showed a CD trough and an S-shaped CD, respectively, associated with the absorption peak at 450 nm, the heme vicinities are remarkably different from each other. The CD spectra in the visible region are also discussed. It was noticed that P-420, the denatured form of P-450, exhibited no CD spectra in the Soret and visible regions."}
{"id": "PMID:465525", "title": "The binding of calcium to fibrinogen: influence on the clotting process.", "content": "The influence of Ca2+ on the basic reaction between thrombin and fibrinogen was investigated. The results demonstrate that: (a) A Ca2+-dependent dimeric intermediate is formed during the early step of the clotting process. This dimeric intermeidate is shown to be formed by the association of an intact fibrinogen molecule and a fibrin monomer devoid in only the peptide A, (b) Ca2+ enhances the proteolytic step as illustrated by the measure of the kinetics of H+ release at pH 8.6. On the basis of these observations it is proposed that Ca2+ catalyses the proteolysis of fibrinogen by thrombin through the formation of a Ca2+-dependent dimer.", "contents": "The binding of calcium to fibrinogen: influence on the clotting process. The influence of Ca2+ on the basic reaction between thrombin and fibrinogen was investigated. The results demonstrate that: (a) A Ca2+-dependent dimeric intermediate is formed during the early step of the clotting process. This dimeric intermeidate is shown to be formed by the association of an intact fibrinogen molecule and a fibrin monomer devoid in only the peptide A, (b) Ca2+ enhances the proteolytic step as illustrated by the measure of the kinetics of H+ release at pH 8.6. On the basis of these observations it is proposed that Ca2+ catalyses the proteolysis of fibrinogen by thrombin through the formation of a Ca2+-dependent dimer."}
{"id": "PMID:465526", "title": "Molecular weight and structural studies on cephalopod rhodopsin.", "content": "The protein moiety of squid (Watasenia scintillans) rhodopsin has been shown to have a molecular weight of 46 800 by means of amino acid analysis. This value was comparable to the value (51 000) obtained from SDS-polyacrylamide gel electrophoresis. After the squid eyes were incubated at 10 degrees C for 8 days, the rhodopsin showed a molecular weight of 39 000 on electrophoresis. The smaller molecular weight was ascertained by amino acid analysis of the rhodopsin; and may result from autolysis by the lysosomal enzyme. The rhodopsin in rhabdomeric membranes and in detergent solution was treated with chymotrypsin, papain or subtilisin. These enzymes first produced the 39 000 dalton rhodopsin and then cleaved this into the 25 000 and 14 000 dalton peptides without bleaching. The rhodopsin was attacked by proteases and readily lost an approx. 12 000 dalton peptide portion. This portion included the COOH-terminal and was rich in glutamic acid, proline, glycine, alanine and tyrosine residues.", "contents": "Molecular weight and structural studies on cephalopod rhodopsin. The protein moiety of squid (Watasenia scintillans) rhodopsin has been shown to have a molecular weight of 46 800 by means of amino acid analysis. This value was comparable to the value (51 000) obtained from SDS-polyacrylamide gel electrophoresis. After the squid eyes were incubated at 10 degrees C for 8 days, the rhodopsin showed a molecular weight of 39 000 on electrophoresis. The smaller molecular weight was ascertained by amino acid analysis of the rhodopsin; and may result from autolysis by the lysosomal enzyme. The rhodopsin in rhabdomeric membranes and in detergent solution was treated with chymotrypsin, papain or subtilisin. These enzymes first produced the 39 000 dalton rhodopsin and then cleaved this into the 25 000 and 14 000 dalton peptides without bleaching. The rhodopsin was attacked by proteases and readily lost an approx. 12 000 dalton peptide portion. This portion included the COOH-terminal and was rich in glutamic acid, proline, glycine, alanine and tyrosine residues."}
{"id": "PMID:465527", "title": "Mouse hepatoma and liver ferritins. Comparative structural studies.", "content": "Pure ferritin from male mouse liver produces a single band of monomers (RF = 0.199) with electrophoresis in polyacrylamide gels at pH 9.0. The five sub-bands within this monomeric band appear to represent charge isomers having the same molecular size. Ferritin from BH3 transplantable mouse hepatoma shows two overlapping bands of monomers (RFA = 0.208 and RFB = 0.240); further electrophoretic studies show that these bands represent two subpopulations of molecules differing both in charge and size. Sub-bands are not found in this hepatoma ferritin. The larger tumor ferritin reaches the same end migration position as all liver isoferritins on gradient gels, signifying a very similar or identical molecular size; however, the absence of sub-bands indicates that this hepatoma ferritin differs in charge from the homologous liver proteins. Liver and hepatoma ferritins both produce a single prominent subunit band corresponding to nominal molecular weights of 22 250 and 21 700, with polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and dithiothreitol. With electrophoresis on polyacrylamide gradient slabs containing sodium dodecyl sulfate and dithiothreitol, both liver and hepatoma ferritins now reveal two subunits bands situated at identical positions. The polypeptides of these two closely spaced bands have a nominal molecular weight difference of less than 1000. Neither the hepatoma nor the liver seems to produce the ferritins found in the other tissue. Nevertheless, all these ferritins are composed of the same two types of subunits, albeit in different relative amounts. Observed distinctions in the ferritins from these normal or neoplastic cells must reflect differences in assembly and processing, as well as in the regulated expression of the same ferritin genes.", "contents": "Mouse hepatoma and liver ferritins. Comparative structural studies. Pure ferritin from male mouse liver produces a single band of monomers (RF = 0.199) with electrophoresis in polyacrylamide gels at pH 9.0. The five sub-bands within this monomeric band appear to represent charge isomers having the same molecular size. Ferritin from BH3 transplantable mouse hepatoma shows two overlapping bands of monomers (RFA = 0.208 and RFB = 0.240); further electrophoretic studies show that these bands represent two subpopulations of molecules differing both in charge and size. Sub-bands are not found in this hepatoma ferritin. The larger tumor ferritin reaches the same end migration position as all liver isoferritins on gradient gels, signifying a very similar or identical molecular size; however, the absence of sub-bands indicates that this hepatoma ferritin differs in charge from the homologous liver proteins. Liver and hepatoma ferritins both produce a single prominent subunit band corresponding to nominal molecular weights of 22 250 and 21 700, with polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and dithiothreitol. With electrophoresis on polyacrylamide gradient slabs containing sodium dodecyl sulfate and dithiothreitol, both liver and hepatoma ferritins now reveal two subunits bands situated at identical positions. The polypeptides of these two closely spaced bands have a nominal molecular weight difference of less than 1000. Neither the hepatoma nor the liver seems to produce the ferritins found in the other tissue. Nevertheless, all these ferritins are composed of the same two types of subunits, albeit in different relative amounts. Observed distinctions in the ferritins from these normal or neoplastic cells must reflect differences in assembly and processing, as well as in the regulated expression of the same ferritin genes."}
{"id": "PMID:465528", "title": "The prosthetic group of milk lactoperoxidase is protoheme IX.", "content": "The prosthetic group of milk lactoperoxidase has been isolated from a Pronase hydrolysate of the enzyme and identified spectroscopically and chromatographically as protoheme IX. Partial degradation of the heme occurred during the proteolysis, possibly as a result of coupled oxidation in the presence of glycine and oxygen. The heme is assumed to be buried in the protein molecule in a crevice, which allows ligands to bind to the iron on one side only. The pyridine hemochrome of lactoperoxidase with alpha-band at 563 nm is interpreted as a mixed ligand complex with pyridine on one side of the heme and a ligand originating from the protein on the other. No experimental evidence supports the view that the heme is covalently bound to the protein through an ester linkage.", "contents": "The prosthetic group of milk lactoperoxidase is protoheme IX. The prosthetic group of milk lactoperoxidase has been isolated from a Pronase hydrolysate of the enzyme and identified spectroscopically and chromatographically as protoheme IX. Partial degradation of the heme occurred during the proteolysis, possibly as a result of coupled oxidation in the presence of glycine and oxygen. The heme is assumed to be buried in the protein molecule in a crevice, which allows ligands to bind to the iron on one side only. The pyridine hemochrome of lactoperoxidase with alpha-band at 563 nm is interpreted as a mixed ligand complex with pyridine on one side of the heme and a ligand originating from the protein on the other. No experimental evidence supports the view that the heme is covalently bound to the protein through an ester linkage."}
{"id": "PMID:465530", "title": "Salt and temperature-dependent conformation changes in spectrin from human erythrocyte membranes.", "content": "ORD and CD measurements of spectrin, in both the dimer and tetramer association state, indicate a high proportion of alpha-helix in this protein. At temperatures below 27 degrees C and in 0.1 M NaCl, the tetramer has an apparent helix content of 73% and the dimer, 68%. The conformation of both states is dependent on salt concentration and temperature. Low ionic strength solutions of spectrin display lowered sedimentation coefficients and a decreased apparent helix content, indicating perhaps a slight refolding and expansion of the molecule. In addition, spectrin in low ionic strength solutions undergoes a broad temperature-dependent transition spread from 20 to 50 degrees C, while in the presence of salt the transition is sharp and centered on 49 degrees C. The temperature-dependent changes in low ionic strength solutions appear to parallel the dissociation of tetramer to dimer.", "contents": "Salt and temperature-dependent conformation changes in spectrin from human erythrocyte membranes. ORD and CD measurements of spectrin, in both the dimer and tetramer association state, indicate a high proportion of alpha-helix in this protein. At temperatures below 27 degrees C and in 0.1 M NaCl, the tetramer has an apparent helix content of 73% and the dimer, 68%. The conformation of both states is dependent on salt concentration and temperature. Low ionic strength solutions of spectrin display lowered sedimentation coefficients and a decreased apparent helix content, indicating perhaps a slight refolding and expansion of the molecule. In addition, spectrin in low ionic strength solutions undergoes a broad temperature-dependent transition spread from 20 to 50 degrees C, while in the presence of salt the transition is sharp and centered on 49 degrees C. The temperature-dependent changes in low ionic strength solutions appear to parallel the dissociation of tetramer to dimer."}
{"id": "PMID:465531", "title": "Multiple molecular forms of glia maturation factor.", "content": "Glia maturation factor from the pig brain can be detected in two molecular forms: the high molecular weight form which is 200 000 dalton in size and the low molecular weight form which is 40 000 dalton in size, as determined by Sephadex gel filtration. The former accounts for 85% of the total biological activity extracted at physiologic pH. The proportion of the low molecular weight form increases following freeze-thawing and ion-exchange chromatography. In addition to the morphological effects, both forms possess mitogenic activity but no esteropeptidase activity. Both forms show similar enzyme susceptibility, being inactivated by papain, ficin and pronase but resistant to subtilisin, thermolysin and trypsin. The high molecular weight form is more resistant to denaturation by low pH, heating and urea than the low molecular weight form. The high molecular weight factor has an isoelectric point of 4.27 whereas the low molecular weight factor has one of 5.04.", "contents": "Multiple molecular forms of glia maturation factor. Glia maturation factor from the pig brain can be detected in two molecular forms: the high molecular weight form which is 200 000 dalton in size and the low molecular weight form which is 40 000 dalton in size, as determined by Sephadex gel filtration. The former accounts for 85% of the total biological activity extracted at physiologic pH. The proportion of the low molecular weight form increases following freeze-thawing and ion-exchange chromatography. In addition to the morphological effects, both forms possess mitogenic activity but no esteropeptidase activity. Both forms show similar enzyme susceptibility, being inactivated by papain, ficin and pronase but resistant to subtilisin, thermolysin and trypsin. The high molecular weight form is more resistant to denaturation by low pH, heating and urea than the low molecular weight form. The high molecular weight factor has an isoelectric point of 4.27 whereas the low molecular weight factor has one of 5.04."}
{"id": "PMID:465532", "title": "Some properties and the complete primary structures of two reduced and S-carboxymethylated polypeptides (S5C1 and S5C10) from Dendroaspis jamesoni kaimosae (Jameson's mamba) venom.", "content": "Two polypeptides (protein S5C1 and toxin S5C10) were purified from Dendroaspis jamesoni kaimosae venom. Whereas protein S5C1 comprises 61 amino acid residues, toxin S5C10 contains 58 and they each comprise four disulphide bridges. The complete primary structures of the two polypeptides have been elucidated. The sequences of protein S5C1 and toxin S5C10 are structurally homologous to the short neurotoxins Type I, but they are much less toxic. In toxin S5C10 one of the functionally invariant amino acid residues, lysine 26, of the Type I neurotoxin has been replaced by a serine. In contrast protein S5C1 has the feature that it contains ten or eleven structurally invariant amino acids and apparently only one of the five functionally invariant residues.", "contents": "Some properties and the complete primary structures of two reduced and S-carboxymethylated polypeptides (S5C1 and S5C10) from Dendroaspis jamesoni kaimosae (Jameson's mamba) venom. Two polypeptides (protein S5C1 and toxin S5C10) were purified from Dendroaspis jamesoni kaimosae venom. Whereas protein S5C1 comprises 61 amino acid residues, toxin S5C10 contains 58 and they each comprise four disulphide bridges. The complete primary structures of the two polypeptides have been elucidated. The sequences of protein S5C1 and toxin S5C10 are structurally homologous to the short neurotoxins Type I, but they are much less toxic. In toxin S5C10 one of the functionally invariant amino acid residues, lysine 26, of the Type I neurotoxin has been replaced by a serine. In contrast protein S5C1 has the feature that it contains ten or eleven structurally invariant amino acids and apparently only one of the five functionally invariant residues."}
{"id": "PMID:465533", "title": "Selective extraction of desmosomal proteins by low ionic strength media.", "content": "Desmosomes, isolated using an acidic buffer, have been subjected to extraction at low ionic strength. This treatment removes more than 35% of their protein in the form of two polypeptide chains of molecular weight 210 000 and 230 000, but the desmosomes show only subtle changes in ultrastructure. It is concluded that the use of low ionic strength media for desmosome isolation yields residual structures specifically depleted in high molecular weight proteins.", "contents": "Selective extraction of desmosomal proteins by low ionic strength media. Desmosomes, isolated using an acidic buffer, have been subjected to extraction at low ionic strength. This treatment removes more than 35% of their protein in the form of two polypeptide chains of molecular weight 210 000 and 230 000, but the desmosomes show only subtle changes in ultrastructure. It is concluded that the use of low ionic strength media for desmosome isolation yields residual structures specifically depleted in high molecular weight proteins."}
{"id": "PMID:465534", "title": "Purification of human transcobalamin II-cyanocobalamin by affinity chromatography using thermolabile immobilization of cyanocobalamin.", "content": "Transcobalamin II-cyanocobalamin was isolated from Cohn fraction III of pooled human plasma by affinity chromatography on cyanocobalamin-Sepharose and some conventional separation methods. The affinity ligand cyanocobalamin was coupled to AH-Sepharose by a thermolabile linkage. The unsaturated binding protein was absorbed at 4 degrees C and eluted from the column at 37 degrees C as transcobalamin II-cyanocobalamin complex. The final preparation had a specific cyanocobalamin-binding capacity of 0.98 mol cyanocobalamin/mol transcobalamin II, the yield was 55% and the purification index amounted to 1.1 . 10(6). In dodecyl sulphate polyacrylamide gel electrophoresis one major protein band was observed at a molecular weight of 37 000 and a faint band at a molecular weight of 29 000. In polyacrylamide gel isolectric focusing the pure preparation turned out to be heterogeneous with isoelectric points ranging from pH 6.2 to 6.8, possibly by the occurrence of isoproteins.", "contents": "Purification of human transcobalamin II-cyanocobalamin by affinity chromatography using thermolabile immobilization of cyanocobalamin. Transcobalamin II-cyanocobalamin was isolated from Cohn fraction III of pooled human plasma by affinity chromatography on cyanocobalamin-Sepharose and some conventional separation methods. The affinity ligand cyanocobalamin was coupled to AH-Sepharose by a thermolabile linkage. The unsaturated binding protein was absorbed at 4 degrees C and eluted from the column at 37 degrees C as transcobalamin II-cyanocobalamin complex. The final preparation had a specific cyanocobalamin-binding capacity of 0.98 mol cyanocobalamin/mol transcobalamin II, the yield was 55% and the purification index amounted to 1.1 . 10(6). In dodecyl sulphate polyacrylamide gel electrophoresis one major protein band was observed at a molecular weight of 37 000 and a faint band at a molecular weight of 29 000. In polyacrylamide gel isolectric focusing the pure preparation turned out to be heterogeneous with isoelectric points ranging from pH 6.2 to 6.8, possibly by the occurrence of isoproteins."}
{"id": "PMID:465535", "title": "The isolectins of Helix pomatia. Separation by isoelectric focusing and preliminary characterization.", "content": "Helix pomatia lectin was fractionated into several (at least 12) components by isolectric focusing in PharmalyteTM. Eight of these were recovered from a preparative column experiment and shown to be essentially pure by analytical electrofocusing in polyacrylamide gel slabs. The lectin components had haemagglutinating activity against human group A erythrocytes and similar molecular weights of 80 000 as determined vidual components showed slight variations in composition. The components are considered to be isolectins. Isoelectric focusing of lectin samples prepared from single snails from two different biotopes showed isolectin patterns similar to that of pooled lectin. Gel filtration of the lectin and flat bed electrofocusing of isolated isolectins both in the presence of 6 M ura, indicated that the lectin molecules are formed by different combinations of a range subunits with similar molecular weight but with different isoelectric points.", "contents": "The isolectins of Helix pomatia. Separation by isoelectric focusing and preliminary characterization. Helix pomatia lectin was fractionated into several (at least 12) components by isolectric focusing in PharmalyteTM. Eight of these were recovered from a preparative column experiment and shown to be essentially pure by analytical electrofocusing in polyacrylamide gel slabs. The lectin components had haemagglutinating activity against human group A erythrocytes and similar molecular weights of 80 000 as determined vidual components showed slight variations in composition. The components are considered to be isolectins. Isoelectric focusing of lectin samples prepared from single snails from two different biotopes showed isolectin patterns similar to that of pooled lectin. Gel filtration of the lectin and flat bed electrofocusing of isolated isolectins both in the presence of 6 M ura, indicated that the lectin molecules are formed by different combinations of a range subunits with similar molecular weight but with different isoelectric points."}
{"id": "PMID:465536", "title": "Separation of the integral membrane glycoproteins E1 and E2 of Semliki Forest virus by affinity chromatography on concanavalin A-Sepharose.", "content": "The membrane glycoproteins E1 and E2 of Semliki Forest virus are of about equal size but can be separated from each other by affinity chromatography on a concanavalin A-Sepharose column in the presence of sodium dodecyl sulfate. The E1 protein eluted like glycopeptides containing two peripheral sugar branches composed of N-acetylglucosamine, mannose, galactose and sialic acid. The E2 eluted like glycopeptides containing only N-acetylglucosamine and mannose.", "contents": "Separation of the integral membrane glycoproteins E1 and E2 of Semliki Forest virus by affinity chromatography on concanavalin A-Sepharose. The membrane glycoproteins E1 and E2 of Semliki Forest virus are of about equal size but can be separated from each other by affinity chromatography on a concanavalin A-Sepharose column in the presence of sodium dodecyl sulfate. The E1 protein eluted like glycopeptides containing two peripheral sugar branches composed of N-acetylglucosamine, mannose, galactose and sialic acid. The E2 eluted like glycopeptides containing only N-acetylglucosamine and mannose."}
{"id": "PMID:465537", "title": "Isolation and partial characterization of the major glycoprotein from the plasma membranes of AH-66 hepatoma cells.", "content": "A major glycoprotein of the plasma membranes of AH-66 hepatoma ascites cells was isolated in essentially pure form and in milligram amounts. The plasma membranes were solubilized with a solution containing both 0.3 M lithium diiodosalycylate and 0.2% cetylpyridinium chloride, and further extracted with 50% phenol, followed by gel filtration on Sepharose 6B in the presence of 0.1% Ammonyx-LO at pH 8.0. The apparent molecular weight of the purified glycoprotein was estimated to be 165 000 in 5.6% polyacrylamide gels, of which 54% was carbohydrate and 46% was protein. The chemical composition of the glycoprotein resembles glycophorin A from human erythrocyte membranes in that it has a high content of N-acetylgalactosamine, N-acetylglucosamine, galactose and sialic acid and a particularly large proportion of serine, threonine, aspartic acid and glutamic acid.", "contents": "Isolation and partial characterization of the major glycoprotein from the plasma membranes of AH-66 hepatoma cells. A major glycoprotein of the plasma membranes of AH-66 hepatoma ascites cells was isolated in essentially pure form and in milligram amounts. The plasma membranes were solubilized with a solution containing both 0.3 M lithium diiodosalycylate and 0.2% cetylpyridinium chloride, and further extracted with 50% phenol, followed by gel filtration on Sepharose 6B in the presence of 0.1% Ammonyx-LO at pH 8.0. The apparent molecular weight of the purified glycoprotein was estimated to be 165 000 in 5.6% polyacrylamide gels, of which 54% was carbohydrate and 46% was protein. The chemical composition of the glycoprotein resembles glycophorin A from human erythrocyte membranes in that it has a high content of N-acetylgalactosamine, N-acetylglucosamine, galactose and sialic acid and a particularly large proportion of serine, threonine, aspartic acid and glutamic acid."}
{"id": "PMID:465538", "title": "Preparation of cyanogen bromide fragments of MM, NN, and MN glycoproteins (glycophorins) from human erythrocyte membranes of single donors.", "content": "A procedure is described for the preparation of three cyanogen bromide fragments of the MM, NN, or MN glycoprotein (glycophorin) of the human erythrocyte membranes, from erythrocytes of single donors. The fragments are obtained in pure form and excellent yields by employing procedures which include proteolytic inhibitors during membrane processing, thorough delipidation of the glycoprotein, and CNBr cleavage conditions which lead to quantitative fragmentation without loss of carbohydrates. A phenol-urea extraction resolves the two glycopeptide fragments from the carbohydrate-free fragment. The two glycopeptides are further purified by Bio-Gel P-6 and P-100 chromatography. The three fragments include the amino terminal 8 residue glycopeptide, a large glycopeptide form the middle of the molecule which bears the Asn-linked oligosaccharide and 8--9 O-glycosidically linked units, and a carboxyl terminal, carbohydrate-free, approx. 50 residue fragment. Their amino acid and carbohydrate composition, and size, are in close agreement with the sequence data of Tomita, M., Furthmayr, H. and Marchesi, V.T. (Biochemistry (1978), 17, 4756--4770). The fragments represent three well delineated portions of the glycoprotein molecule.", "contents": "Preparation of cyanogen bromide fragments of MM, NN, and MN glycoproteins (glycophorins) from human erythrocyte membranes of single donors. A procedure is described for the preparation of three cyanogen bromide fragments of the MM, NN, or MN glycoprotein (glycophorin) of the human erythrocyte membranes, from erythrocytes of single donors. The fragments are obtained in pure form and excellent yields by employing procedures which include proteolytic inhibitors during membrane processing, thorough delipidation of the glycoprotein, and CNBr cleavage conditions which lead to quantitative fragmentation without loss of carbohydrates. A phenol-urea extraction resolves the two glycopeptide fragments from the carbohydrate-free fragment. The two glycopeptides are further purified by Bio-Gel P-6 and P-100 chromatography. The three fragments include the amino terminal 8 residue glycopeptide, a large glycopeptide form the middle of the molecule which bears the Asn-linked oligosaccharide and 8--9 O-glycosidically linked units, and a carboxyl terminal, carbohydrate-free, approx. 50 residue fragment. Their amino acid and carbohydrate composition, and size, are in close agreement with the sequence data of Tomita, M., Furthmayr, H. and Marchesi, V.T. (Biochemistry (1978), 17, 4756--4770). The fragments represent three well delineated portions of the glycoprotein molecule."}
{"id": "PMID:465539", "title": "Precocious differentiation of chick embryo pancreas in vitro. Roles of prednisolone, insulin and L-thyroxine.", "content": "The hormonal requirement for functional differentiation of chick embryo pancreas were investigated by using organ cultures in chemically defined medium. The hormones tested were prednisolone, insulin and thyroxine, and the parameters examined were alpha-amylase (EC 3.2.1.1) and chymotrypsinogen (EC 3.4.4.5) activities, and the ultrastructure of the tissues. Addition of prednisolone alone to explants from 14-day-old chicken embryo pancreas for 3 days increased the activities of amylase and chymotrypsinogen in the tissues by 3.4- and 6.6-fold, respectively, those of tissues before cultivation. Neither thyroxine or insulin alone, nor both hormones together affected pancreatic exocrine differentiation. Thyroxine enhanced the effect of prednisolone on both enzymes, but insulin did not. When the explants were cultured in the medium containing all three hormones, maximum enzyme activities were observed; amylase or chymotrypsinogen activity being 7- or 18-fold, respectively, that of tissues before cultivation. But these three hormones were not simultaneously necessary. Morphological differentiation was also observed in explants cultuvated in medium containing these three hormones. These results suggest that glucocorticoids are essential for normal differentiation of chick pancreas during the late fetal period, possibly with insulin and thyroxine, and also support the idea that pancreatic enzymes are controlled separately.", "contents": "Precocious differentiation of chick embryo pancreas in vitro. Roles of prednisolone, insulin and L-thyroxine. The hormonal requirement for functional differentiation of chick embryo pancreas were investigated by using organ cultures in chemically defined medium. The hormones tested were prednisolone, insulin and thyroxine, and the parameters examined were alpha-amylase (EC 3.2.1.1) and chymotrypsinogen (EC 3.4.4.5) activities, and the ultrastructure of the tissues. Addition of prednisolone alone to explants from 14-day-old chicken embryo pancreas for 3 days increased the activities of amylase and chymotrypsinogen in the tissues by 3.4- and 6.6-fold, respectively, those of tissues before cultivation. Neither thyroxine or insulin alone, nor both hormones together affected pancreatic exocrine differentiation. Thyroxine enhanced the effect of prednisolone on both enzymes, but insulin did not. When the explants were cultured in the medium containing all three hormones, maximum enzyme activities were observed; amylase or chymotrypsinogen activity being 7- or 18-fold, respectively, that of tissues before cultivation. But these three hormones were not simultaneously necessary. Morphological differentiation was also observed in explants cultuvated in medium containing these three hormones. These results suggest that glucocorticoids are essential for normal differentiation of chick pancreas during the late fetal period, possibly with insulin and thyroxine, and also support the idea that pancreatic enzymes are controlled separately."}
{"id": "PMID:465540", "title": "Effect of insulin on glucose oxidation and amino isobutyric acid transport and binding of insulin in chicken thymocytes.", "content": "In chicken thymocytes isolated from 15--40 day-old chickens, after a 2 h incubation at 37 degrees C, insulin stimulated amino isobutyric acid uptake (maximal response: 40--50% of increase at 1 microgram insulin/ml and half maximal response at 60 ng/ml) by specifically stimulating the influx without altering the efflux. Insulin also stimulated glucose oxidation (maximal response: 11% of increase at 1 microgram insulin/ml). Binding of 125I-labelled chicken insulin to thymocytes was rapid and higher at 15 degrees C than at 37 degrees C. At steady state, (90 min at 15 degrees C), chicken, porcine and goose insulins were equipotent in inhibiting the binding of 125I-labelled chicken insulin. Maximal binding capacity was estimated at 1250 pg insulin/10(8) cells, i.e., 1250 binding sites/cell with an apparent dissociation constant of 200 ng insulin/ml at 15 degrees C. Degradation of 125I-labelled chicken insulin in the incubation medium was negligible at 15 degrees C but very noticeable at 37 degrees C. Therefore, the low level of insulin binding at 15 degrees C reflects a true scarcity of insulin receptors in chicken thymocytes as compared to rat thymocytes.", "contents": "Effect of insulin on glucose oxidation and amino isobutyric acid transport and binding of insulin in chicken thymocytes. In chicken thymocytes isolated from 15--40 day-old chickens, after a 2 h incubation at 37 degrees C, insulin stimulated amino isobutyric acid uptake (maximal response: 40--50% of increase at 1 microgram insulin/ml and half maximal response at 60 ng/ml) by specifically stimulating the influx without altering the efflux. Insulin also stimulated glucose oxidation (maximal response: 11% of increase at 1 microgram insulin/ml). Binding of 125I-labelled chicken insulin to thymocytes was rapid and higher at 15 degrees C than at 37 degrees C. At steady state, (90 min at 15 degrees C), chicken, porcine and goose insulins were equipotent in inhibiting the binding of 125I-labelled chicken insulin. Maximal binding capacity was estimated at 1250 pg insulin/10(8) cells, i.e., 1250 binding sites/cell with an apparent dissociation constant of 200 ng insulin/ml at 15 degrees C. Degradation of 125I-labelled chicken insulin in the incubation medium was negligible at 15 degrees C but very noticeable at 37 degrees C. Therefore, the low level of insulin binding at 15 degrees C reflects a true scarcity of insulin receptors in chicken thymocytes as compared to rat thymocytes."}
{"id": "PMID:465541", "title": "Pattern of glycosaminoglycans and glycoproteins associated with nuclei of regenerating liver of rat.", "content": "1. Hyaluronic acid was detected as the largest glycosaminoglycan component in the glycosaminoglycan fraction from purified nuclei of regenerating livers as in the case of normal livers (Furukawa, K. and Tarayama, H. (1977) Biochim. Biophys. Acta 499, 278--289). However, the nuclear content of glycosaminoglycans tended to decrease after partial hepatectomy, reaching one-third of the normal liver level at 24--30 h after partial hepatectomy. On the other hand, two new polyanionic components were detected in the glycosaminoglycan fraction from regenerating liver nuclei. 2. One of these new components seems to be a sulfated glycopeptide. The 35SO4 incorporation into this component was stimulated biphasically after partial hepatectomy; the first stimulation occurring immediately after partial hepatectomy and the second stimulation occurring almost in parallel to the DNA synthesis. 3. Another polyacnionic component which also increases in the nuclear content after partial hepatectomy lacks hexuronic acid, sialic acid and 35SO4 and yet it is intensely stained by Alcian Blue. Preliminary investigations revealed the presence of hexose, ribose and phosphate as the major components. 4. In contrast to the primary localization of hyaluronic acid in the chromatin fraction and also in the nonhistone chromosomal protein fraction from it, these new polyanionic components were detected mainly in the karyosol fraction.", "contents": "Pattern of glycosaminoglycans and glycoproteins associated with nuclei of regenerating liver of rat. 1. Hyaluronic acid was detected as the largest glycosaminoglycan component in the glycosaminoglycan fraction from purified nuclei of regenerating livers as in the case of normal livers (Furukawa, K. and Tarayama, H. (1977) Biochim. Biophys. Acta 499, 278--289). However, the nuclear content of glycosaminoglycans tended to decrease after partial hepatectomy, reaching one-third of the normal liver level at 24--30 h after partial hepatectomy. On the other hand, two new polyanionic components were detected in the glycosaminoglycan fraction from regenerating liver nuclei. 2. One of these new components seems to be a sulfated glycopeptide. The 35SO4 incorporation into this component was stimulated biphasically after partial hepatectomy; the first stimulation occurring immediately after partial hepatectomy and the second stimulation occurring almost in parallel to the DNA synthesis. 3. Another polyacnionic component which also increases in the nuclear content after partial hepatectomy lacks hexuronic acid, sialic acid and 35SO4 and yet it is intensely stained by Alcian Blue. Preliminary investigations revealed the presence of hexose, ribose and phosphate as the major components. 4. In contrast to the primary localization of hyaluronic acid in the chromatin fraction and also in the nonhistone chromosomal protein fraction from it, these new polyanionic components were detected mainly in the karyosol fraction."}
{"id": "PMID:465542", "title": "Collagen synthesis by cultured skin fibroblasts from siblings with hydroxylysine-deficient collagen.", "content": "It has been previously shown that dermis from subjects with hydroxylysine-deficient collagen contains approximately 5% of normal levels of hydroxylysine and sonicates of skin fibroblasts contain less than 15% of normal levels of collagen lysyl hydroxylase activity. However, cultures of dermal fibroblasts from two siblings with hydroxylysine-deficient collagen (Ehlers-Danlos Syndrome Type VI) compared to fibroblasts from normal subjects synthesize collagen containing approximately 50% of normal amounts of hydroxylysine. The lysyl hydroxylase deficient cultures synthesize both Type I and Type III collagen in the same proportion as control cultures. Both alpha 1(I) and alpha 2 chains are similarly reduced in hydroxylysine content. Collagen prolyl hydroxylation by normal collagen lysyl hydroxylation is the same with or without ascorbate supplementation. In mutant cells the rate of prolyl hydroxylation measured after release of inhibition by alpha, alpha'-dipyridyl is the same as in control cells. The rate of lysyl hydroxylation is reduced in mutant cells but only to approximately 50% of normal.", "contents": "Collagen synthesis by cultured skin fibroblasts from siblings with hydroxylysine-deficient collagen. It has been previously shown that dermis from subjects with hydroxylysine-deficient collagen contains approximately 5% of normal levels of hydroxylysine and sonicates of skin fibroblasts contain less than 15% of normal levels of collagen lysyl hydroxylase activity. However, cultures of dermal fibroblasts from two siblings with hydroxylysine-deficient collagen (Ehlers-Danlos Syndrome Type VI) compared to fibroblasts from normal subjects synthesize collagen containing approximately 50% of normal amounts of hydroxylysine. The lysyl hydroxylase deficient cultures synthesize both Type I and Type III collagen in the same proportion as control cultures. Both alpha 1(I) and alpha 2 chains are similarly reduced in hydroxylysine content. Collagen prolyl hydroxylation by normal collagen lysyl hydroxylation is the same with or without ascorbate supplementation. In mutant cells the rate of prolyl hydroxylation measured after release of inhibition by alpha, alpha'-dipyridyl is the same as in control cells. The rate of lysyl hydroxylation is reduced in mutant cells but only to approximately 50% of normal."}
{"id": "PMID:465543", "title": "Preliminary studies on the composition and rheological properties of the extracellular polysaccharide synthesized by Pseudomonas PBI (NCIB 11264).", "content": "A microorganism isolated from a carbohydrate-rich industrial effluent synthesized an exocellular slime polysaccharide composed of glucose and galactose in a molar ratio of 7.45 +/- 0.68 : 1, and two non-carbohydrate substituents acetate (3--4%) and pyruvate (5--9%). Contamination by rhamnose and mannose was detectable in crude polysaccharide samples. Solutions of the polysaccharide were pseudoplastic, but not thixotropic, and formed gels in the presence of certain trivalent cations.", "contents": "Preliminary studies on the composition and rheological properties of the extracellular polysaccharide synthesized by Pseudomonas PBI (NCIB 11264). A microorganism isolated from a carbohydrate-rich industrial effluent synthesized an exocellular slime polysaccharide composed of glucose and galactose in a molar ratio of 7.45 +/- 0.68 : 1, and two non-carbohydrate substituents acetate (3--4%) and pyruvate (5--9%). Contamination by rhamnose and mannose was detectable in crude polysaccharide samples. Solutions of the polysaccharide were pseudoplastic, but not thixotropic, and formed gels in the presence of certain trivalent cations."}
{"id": "PMID:465544", "title": "The effect of oxidant stress on diamide-treated human granulocytes.", "content": "The role of sulfhydryls in the protection of human polymorphonuclear neutrophils against extracellular oxidant attack was investigated by simultaneously exposing polymorphonuclear neutrophils to the thiol-oxidizing agent diamide and the oxidant-generating system xanthine-xanthine oxidase. Neither diamide nor the oxidants generated by the xanthine-xanthine oxidase system alone impaired the burst in chemiluminescence, hexose monophosphate shunt activity or formate oxidation normally seen during polymorphonuclear neutrophil phagocytosis. Incubation of the polymorphonuclear neutrophils simultaneously with diamide and xanthine-xanthine oxidase markedly impaired polymorphonuclear neutrophil phagocytosis, hexose monophosphate shunt activity, chemiluminescence and formate oxidation. Although the polymorphonuclear neutrophils exposed to diamide and xanthine-xanthine oxidase did not respond to a variety of phagocytizable stimuli, trypan blue exclusion was normal and hexose monophosphate shunt activity could be stimulated by diamide. The damaging effect of the diamide xanthine-xamthine oxidase system could be blocked by the addition of superoxide dismutase or catalase, but not by hydroxyl radical or singlet oxygen scavengers. We hypothesize that an unidentified population of thiols may play a role in protecting the polymorphonuclear neutrophil from endogenously derived oxidants.", "contents": "The effect of oxidant stress on diamide-treated human granulocytes. The role of sulfhydryls in the protection of human polymorphonuclear neutrophils against extracellular oxidant attack was investigated by simultaneously exposing polymorphonuclear neutrophils to the thiol-oxidizing agent diamide and the oxidant-generating system xanthine-xanthine oxidase. Neither diamide nor the oxidants generated by the xanthine-xanthine oxidase system alone impaired the burst in chemiluminescence, hexose monophosphate shunt activity or formate oxidation normally seen during polymorphonuclear neutrophil phagocytosis. Incubation of the polymorphonuclear neutrophils simultaneously with diamide and xanthine-xanthine oxidase markedly impaired polymorphonuclear neutrophil phagocytosis, hexose monophosphate shunt activity, chemiluminescence and formate oxidation. Although the polymorphonuclear neutrophils exposed to diamide and xanthine-xanthine oxidase did not respond to a variety of phagocytizable stimuli, trypan blue exclusion was normal and hexose monophosphate shunt activity could be stimulated by diamide. The damaging effect of the diamide xanthine-xamthine oxidase system could be blocked by the addition of superoxide dismutase or catalase, but not by hydroxyl radical or singlet oxygen scavengers. We hypothesize that an unidentified population of thiols may play a role in protecting the polymorphonuclear neutrophil from endogenously derived oxidants."}
{"id": "PMID:465545", "title": "Isolation and identification of S(+)-3-hydroxyisobutyric acid in the urine of rats loaded with isobutyric acid.", "content": "3-Hydroxyisobutyric acid has been isolated from the urine of rats loaded with sodium isobutyrate. An optical rotation measurement of the methyl ester derivative shows this compound to be the S(+) stereoisomer. This is the same stereoisomer that has been previously identified in cultures of bacteria incubated with ammonium isobutyrate (Aberhart, D.J. (1977) Bioorg. Chem. 6, 191--201).", "contents": "Isolation and identification of S(+)-3-hydroxyisobutyric acid in the urine of rats loaded with isobutyric acid. 3-Hydroxyisobutyric acid has been isolated from the urine of rats loaded with sodium isobutyrate. An optical rotation measurement of the methyl ester derivative shows this compound to be the S(+) stereoisomer. This is the same stereoisomer that has been previously identified in cultures of bacteria incubated with ammonium isobutyrate (Aberhart, D.J. (1977) Bioorg. Chem. 6, 191--201)."}
{"id": "PMID:465546", "title": "[Several estimations of the solutions of the system of differential equations describing electron transfer processes].", "content": "Some inequalities are deduced in relation to probabilities of the states of molecules-carriers complex. The existing hierarchy for velocity constants in electron transfer chains permits the application of deduced inequalities both for the simplification of the initial equation system, and for different evaluations, as for example for determining the maximum velocity of electron transport through the complex, for determing the number of electrons in the complex etc.", "contents": "[Several estimations of the solutions of the system of differential equations describing electron transfer processes]. Some inequalities are deduced in relation to probabilities of the states of molecules-carriers complex. The existing hierarchy for velocity constants in electron transfer chains permits the application of deduced inequalities both for the simplification of the initial equation system, and for different evaluations, as for example for determining the maximum velocity of electron transport through the complex, for determing the number of electrons in the complex etc."}
{"id": "PMID:465547", "title": "[Photooxidation and photochemiluminescence of different proteins].", "content": "Information has been obtained concerning photooxidation rate of 18 proteins, blood plasma, glycyl triptophane and some amino acids under UV-irradiation. It has been shown that the majority of the objects studied have the quantum yields of oxygen absorption in the range 0.2--0.6%. Photochemoluminescence of proteins was also investigated. It has been found that protein photochemoluminescence characteristics differ significantly, their main differences are concerned with intramolecular reaction of peroxide free radicals responsible for chemoluminescence with protein acceptors.", "contents": "[Photooxidation and photochemiluminescence of different proteins]. Information has been obtained concerning photooxidation rate of 18 proteins, blood plasma, glycyl triptophane and some amino acids under UV-irradiation. It has been shown that the majority of the objects studied have the quantum yields of oxygen absorption in the range 0.2--0.6%. Photochemoluminescence of proteins was also investigated. It has been found that protein photochemoluminescence characteristics differ significantly, their main differences are concerned with intramolecular reaction of peroxide free radicals responsible for chemoluminescence with protein acceptors."}
{"id": "PMID:465550", "title": "[Two possible mechanisms for forming supramolecular structures of the collagen type].", "content": "Comparative electron microscopic investigation of submolecular structure formation of native collagen molecules and denaturated collagen peptides in tropocollagen and gelatin gels was carried out. Submolecular terminal structures were similar. Fibrils exist with cross striation region of 64--70 nm. In spite of this the intermediate steps in the formation of both structures differ. In case of collagen the bundle of parallel microfibrils forms the thick fibrils. In case of gelatin the aggregates of spherical anisotropic particles form the fibrils of gelatin gels.", "contents": "[Two possible mechanisms for forming supramolecular structures of the collagen type]. Comparative electron microscopic investigation of submolecular structure formation of native collagen molecules and denaturated collagen peptides in tropocollagen and gelatin gels was carried out. Submolecular terminal structures were similar. Fibrils exist with cross striation region of 64--70 nm. In spite of this the intermediate steps in the formation of both structures differ. In case of collagen the bundle of parallel microfibrils forms the thick fibrils. In case of gelatin the aggregates of spherical anisotropic particles form the fibrils of gelatin gels."}
{"id": "PMID:465551", "title": "[Electromechanical model of an enzyme-substrate kompleksa].", "content": "An electro-mechanical model is suggested for studying the hydrolytic reaction catalyzed by an enzyme having a domain structure. The fixation of the necessary set of reagents configurations by the enzyme molecule allows the reaction rate acceleration. The model suggests specificity of the enzyme action. The model makes more clear the phenomenon of complementarity between the unchanging part of the substrate molecule and the active site of the enzyme which require not only the spatial fit but also the agreement of some physical properties, such as atom and group partial charges. The model is selfconsistent, i. e. it is not contrary to the existing data on the structural and functional properties of enzyme proteins having the domain structure.", "contents": "[Electromechanical model of an enzyme-substrate kompleksa]. An electro-mechanical model is suggested for studying the hydrolytic reaction catalyzed by an enzyme having a domain structure. The fixation of the necessary set of reagents configurations by the enzyme molecule allows the reaction rate acceleration. The model suggests specificity of the enzyme action. The model makes more clear the phenomenon of complementarity between the unchanging part of the substrate molecule and the active site of the enzyme which require not only the spatial fit but also the agreement of some physical properties, such as atom and group partial charges. The model is selfconsistent, i. e. it is not contrary to the existing data on the structural and functional properties of enzyme proteins having the domain structure."}
{"id": "PMID:465553", "title": "[Changes in the elastic properties of lipid bilayers as a result of different procedures].", "content": "The bilayer elasticity modulus approximately E perpendicular is sensitive to alternative electrical field, addition of different detergents and proteins incorporation. The detergents modify the value E1APPROXIMATELY E perpendicular] several times, the kinetics of this effect is more frequently biphasic and its shape and time parameters are characterized with a considerable dispersion; in general the [approximately E perpendicular] value increase for soft membranes and decrease for the membranes and high elasticity modulus. The proteins incorporation as well as the destruction of the membrane is accompanied with the general structure transition of the whole membrane. The measurement of the elasticity modulus is an effective test of protein incorporation in bilayers.", "contents": "[Changes in the elastic properties of lipid bilayers as a result of different procedures]. The bilayer elasticity modulus approximately E perpendicular is sensitive to alternative electrical field, addition of different detergents and proteins incorporation. The detergents modify the value E1APPROXIMATELY E perpendicular] several times, the kinetics of this effect is more frequently biphasic and its shape and time parameters are characterized with a considerable dispersion; in general the [approximately E perpendicular] value increase for soft membranes and decrease for the membranes and high elasticity modulus. The proteins incorporation as well as the destruction of the membrane is accompanied with the general structure transition of the whole membrane. The measurement of the elasticity modulus is an effective test of protein incorporation in bilayers."}
{"id": "PMID:465554", "title": "[Reaction between liposomes and biomolecular membranes].", "content": "Small liposomes did not change the resistance of bimolecular lipid membranes (BLM) in monovalent ion solution (less than or equal to 0.5 M). The interaction between liposomes containing cholesterol and BLM free from cholesterol in the solution of polyene antibiotic nystatine induced anion selectivity and smooth growth of BLM conductance by 4--5 orders. The addition of the liposomes containing cholesterol and nystatine induced a step-like fall of BLM resistance by 2--3 orders. Both cation-selective and anion-selective steps was observed. The effects observed point to the fusion and incorporation of liposome into BLM.", "contents": "[Reaction between liposomes and biomolecular membranes]. Small liposomes did not change the resistance of bimolecular lipid membranes (BLM) in monovalent ion solution (less than or equal to 0.5 M). The interaction between liposomes containing cholesterol and BLM free from cholesterol in the solution of polyene antibiotic nystatine induced anion selectivity and smooth growth of BLM conductance by 4--5 orders. The addition of the liposomes containing cholesterol and nystatine induced a step-like fall of BLM resistance by 2--3 orders. Both cation-selective and anion-selective steps was observed. The effects observed point to the fusion and incorporation of liposome into BLM."}
{"id": "PMID:465555", "title": "[High time resolution x-ray study of the dynamics of a single muscle contraction].", "content": "A method of the diffraction cinema which enables to study the time-course of structural changes during twitch contraction is described. The method is based on using synchrotron radiation, position-sensitive counter and small-angle focusing X-ray camera. Only 0.1 s is required to record a good muscle X-ray diagram: meridional diagram contains all layer-lines beginning with the 429 A; the equatorial diagram contains 5 reflections including very weak alpha-reflection. The method allows to record 64 sequent diffraction patterns with different duration (1--2000 ms). The experiment is handled by a computer. Some tens of the films of isometric twitch contraction with time resolution of 3--20 ms have been obtained. During isometric contraction considerable changes in the intensity of both meridional and equatorial reflections were found. The changes were interpreted as indicating movement of cross-bridges toward the thin filaments. During the latent phase there are no visible changes in the intensity of the reflections; the result indicates that during this phase there are no structural changes in position and configuration of cross-bridges.", "contents": "[High time resolution x-ray study of the dynamics of a single muscle contraction]. A method of the diffraction cinema which enables to study the time-course of structural changes during twitch contraction is described. The method is based on using synchrotron radiation, position-sensitive counter and small-angle focusing X-ray camera. Only 0.1 s is required to record a good muscle X-ray diagram: meridional diagram contains all layer-lines beginning with the 429 A; the equatorial diagram contains 5 reflections including very weak alpha-reflection. The method allows to record 64 sequent diffraction patterns with different duration (1--2000 ms). The experiment is handled by a computer. Some tens of the films of isometric twitch contraction with time resolution of 3--20 ms have been obtained. During isometric contraction considerable changes in the intensity of both meridional and equatorial reflections were found. The changes were interpreted as indicating movement of cross-bridges toward the thin filaments. During the latent phase there are no visible changes in the intensity of the reflections; the result indicates that during this phase there are no structural changes in position and configuration of cross-bridges."}
{"id": "PMID:465556", "title": "[Functional role of the structural nonequivalence of the F-actin subunits of thin filaments].", "content": "According to the model of the actin-containing filaments suggested earlier by Frank and author thin filaments of sceletal muscle can be considered as a linear polymer chain composed of structural units each of which can adopt either of two different structural states -- I (inhibited) and A (activated). The structural unit can be represented by a block of two complementary functional units of the thin filaments. The data available indicate that the length of the A-state is slightly longer than the length of the I-state. In this case the relative amount of the functional units in A- and I-states in thin filaments must depend not only on the Ca2+ concentration in sarcomere, but also on the value of the external force pulling the thin filaments in contracting muscle. It is shown that this property of the thin filaments may provide the molecular basis of Hill-equation and the selfadaption of contractile machinery to the value of the external load on muscle.", "contents": "[Functional role of the structural nonequivalence of the F-actin subunits of thin filaments]. According to the model of the actin-containing filaments suggested earlier by Frank and author thin filaments of sceletal muscle can be considered as a linear polymer chain composed of structural units each of which can adopt either of two different structural states -- I (inhibited) and A (activated). The structural unit can be represented by a block of two complementary functional units of the thin filaments. The data available indicate that the length of the A-state is slightly longer than the length of the I-state. In this case the relative amount of the functional units in A- and I-states in thin filaments must depend not only on the Ca2+ concentration in sarcomere, but also on the value of the external force pulling the thin filaments in contracting muscle. It is shown that this property of the thin filaments may provide the molecular basis of Hill-equation and the selfadaption of contractile machinery to the value of the external load on muscle."}
{"id": "PMID:465557", "title": "[Effect of phalloidine on the contractile properties of glycerinized skeletal muscle].", "content": "The phalloidin addition (20--30 mkg/ml) is shown to be followed by complex transient of isometric tension of fibres in low Ca++ concentration. The process is accelerated by the temperature increase. The phalloidin treatment does not cause the loss of contractility and Ca++-sensitivity of isometric tension. The treated fibres lost their ability to work in an autooscillation mode at 20--23 degrees C but were in condition to do this at the temperatures higher than 30 degrees C. The mechanical properties of rigor fibres as well at the myosin aggregation dependence on ionic strength were not markedly changed after the treatment. The results make it possible to conclude that the autooscillation mode of contraction is due to some feedback via I-filaments. The actin modification by phalloidin disturbs this feedback.", "contents": "[Effect of phalloidine on the contractile properties of glycerinized skeletal muscle]. The phalloidin addition (20--30 mkg/ml) is shown to be followed by complex transient of isometric tension of fibres in low Ca++ concentration. The process is accelerated by the temperature increase. The phalloidin treatment does not cause the loss of contractility and Ca++-sensitivity of isometric tension. The treated fibres lost their ability to work in an autooscillation mode at 20--23 degrees C but were in condition to do this at the temperatures higher than 30 degrees C. The mechanical properties of rigor fibres as well at the myosin aggregation dependence on ionic strength were not markedly changed after the treatment. The results make it possible to conclude that the autooscillation mode of contraction is due to some feedback via I-filaments. The actin modification by phalloidin disturbs this feedback."}
{"id": "PMID:465566", "title": "Oocyte growth.", "content": "The main elements of reproduction are exposed with emphasis on its preparation during the larval stage. The system oocyte-nurse cells-follicular cells is described and the principal features of egg morphogenesis are indicated. Two types of problems are concentrated on : 1) sequential determination of growth and evolution of the follicle in the ovarian tube, 2) morphological and biochemical aspects of chorion formation. Variation in the functioning of the oocyte's morphogenetic system is explored with discussion of the role of larval alimentation, temperature, hormonal environment and genetic factors.", "contents": "Oocyte growth. The main elements of reproduction are exposed with emphasis on its preparation during the larval stage. The system oocyte-nurse cells-follicular cells is described and the principal features of egg morphogenesis are indicated. Two types of problems are concentrated on : 1) sequential determination of growth and evolution of the follicle in the ovarian tube, 2) morphological and biochemical aspects of chorion formation. Variation in the functioning of the oocyte's morphogenetic system is explored with discussion of the role of larval alimentation, temperature, hormonal environment and genetic factors."}
{"id": "PMID:465567", "title": "Studies on the anesthetic mechanism of carbon dioxide by using Bombyx mori larvae.", "content": "The study of heartbeat variations of Bombyx mori larvae submitted to CO2 narcosis has provided new information on carbon dioxide anesthesia. Purely anoxiant action must be dismissed. Action at a nerve level is possible since CO2 produces the same effect as ether, which is a classical anesthetic.", "contents": "Studies on the anesthetic mechanism of carbon dioxide by using Bombyx mori larvae. The study of heartbeat variations of Bombyx mori larvae submitted to CO2 narcosis has provided new information on carbon dioxide anesthesia. Purely anoxiant action must be dismissed. Action at a nerve level is possible since CO2 produces the same effect as ether, which is a classical anesthetic."}
{"id": "PMID:465562", "title": "[Luminescence emitted from the second excited electron state (S2) of chlorophyll a].", "content": "A weak emission band at 582 nm in the spectrum of chlorophyll a in ether was observed under the 568.1 nm laser excitation. By means of the excitation spectra and using the method of fluorescence detection of triplet state this band was assigned to the radiative transition S2 leads to S0 of the chlorophyll a molecule. The evaluated value for the lifetime of the excited state S2 is about 0.3 to 0.8 ns.", "contents": "[Luminescence emitted from the second excited electron state (S2) of chlorophyll a]. A weak emission band at 582 nm in the spectrum of chlorophyll a in ether was observed under the 568.1 nm laser excitation. By means of the excitation spectra and using the method of fluorescence detection of triplet state this band was assigned to the radiative transition S2 leads to S0 of the chlorophyll a molecule. The evaluated value for the lifetime of the excited state S2 is about 0.3 to 0.8 ns."}
{"id": "PMID:465568", "title": "Hemocytes and hemocytopoiesis in Silkworms.", "content": "A brief review is presented of the current state of ultrastructure, cytochemistry, and physiology of the hemocytes and meso- and metathoracic peri-imaginal-wing organs in silkworms. According to the accepted morphological classification, five circulating types of hemocytes are recognized in Bombyx mori as well as in Antheraea pernyi. They are prophemocytes or stem cells, plasmatocytes or pre-differentiated cells and three specialized cells, granulocytes, spherule cells and oenocytoids. During post-embryonic development the last four types are the most common in the circulating hemolymph. Plasmatocytes are considered to be pluripotent cells from which granulocytes, spherule cells and oenocytoids are derived. Contrary to the situation in most insects the plasmatocytes are not phagocytic in Antheraea. The granulocytes are efficient phagocytes. Both plasmatocytes and granulocytes are involved in pinocytosis. Another possible function of the granulocytes is hemolymph coagulation. The function of the spherule cells which contain a paracrystalline material (muco- or glycoproteins) is by no means clear. The phenoloxidase activity found within the cytosol of oenocytoids appears effective against the natural monophenol and diphenol substrates. The involvement of oenocytoids in the complex metabolism of phenols and particularly in the production of plasma phenolases has been reported. The mitotic division of five circulating hemocyte types is well known and was long regarded as the only mechanism of postembryonic hemocyte production. We present for silkworms, experimental evidence of the hemocytopoietic function of the meso- and metathoracic organs surrounding the imaginal wing discs. Ablation experiments demonstrate that the mitotic activity of free hemocytes is unable to maintain the normal hemocytogram in the absence of the two paris of organs. These organs are typically divided into cell islets ensheathed by a connective tissue membrane. Two types of islets may be classified by the disposition of the cells : the compact islets or aggregations of stem cells and the reticulate islets which are mainly composed of hemocytes at different steps of differentiation. The relative number of prohemocytes in the total hemocyte population ranges from 84 to 97 p. cent in organs of Antheraea pernyi. This well-defined cell type appears to be the major hemocyte type in hemocytopoietic organs. In Antheraea, the mitotic index (the relative number of mitotic hemocytes in the total cell population) varies from 0.5 to about 3 p. cent. Finally, our data direct attention to cyclic functional changes such as mitotic divisions and hemocyte differentiation which run parallel to the molting cycle.", "contents": "Hemocytes and hemocytopoiesis in Silkworms. A brief review is presented of the current state of ultrastructure, cytochemistry, and physiology of the hemocytes and meso- and metathoracic peri-imaginal-wing organs in silkworms. According to the accepted morphological classification, five circulating types of hemocytes are recognized in Bombyx mori as well as in Antheraea pernyi. They are prophemocytes or stem cells, plasmatocytes or pre-differentiated cells and three specialized cells, granulocytes, spherule cells and oenocytoids. During post-embryonic development the last four types are the most common in the circulating hemolymph. Plasmatocytes are considered to be pluripotent cells from which granulocytes, spherule cells and oenocytoids are derived. Contrary to the situation in most insects the plasmatocytes are not phagocytic in Antheraea. The granulocytes are efficient phagocytes. Both plasmatocytes and granulocytes are involved in pinocytosis. Another possible function of the granulocytes is hemolymph coagulation. The function of the spherule cells which contain a paracrystalline material (muco- or glycoproteins) is by no means clear. The phenoloxidase activity found within the cytosol of oenocytoids appears effective against the natural monophenol and diphenol substrates. The involvement of oenocytoids in the complex metabolism of phenols and particularly in the production of plasma phenolases has been reported. The mitotic division of five circulating hemocyte types is well known and was long regarded as the only mechanism of postembryonic hemocyte production. We present for silkworms, experimental evidence of the hemocytopoietic function of the meso- and metathoracic organs surrounding the imaginal wing discs. Ablation experiments demonstrate that the mitotic activity of free hemocytes is unable to maintain the normal hemocytogram in the absence of the two paris of organs. These organs are typically divided into cell islets ensheathed by a connective tissue membrane. Two types of islets may be classified by the disposition of the cells : the compact islets or aggregations of stem cells and the reticulate islets which are mainly composed of hemocytes at different steps of differentiation. The relative number of prohemocytes in the total hemocyte population ranges from 84 to 97 p. cent in organs of Antheraea pernyi. This well-defined cell type appears to be the major hemocyte type in hemocytopoietic organs. In Antheraea, the mitotic index (the relative number of mitotic hemocytes in the total cell population) varies from 0.5 to about 3 p. cent. Finally, our data direct attention to cyclic functional changes such as mitotic divisions and hemocyte differentiation which run parallel to the molting cycle."}
{"id": "PMID:465561", "title": "[Use of the prompt identification principle in controlling complex motor systems (speed control)].", "content": "Some aspects of the adaptive control of animal motor system described in the form of differential equations system were discussed. It was considered that the system of motor control is a multilevel one in which each level has a definite range of correction capacity for movement deviations. Since the time of the sensory signal passing over the control nervous loop is comparable with the duration of the movement controlled, a mechanism of the fast adaptation to a nonstationary situation was suggested. This mechanism is based on the principle of urgent identification of the motor system state at the moment of movement initiation. Urgent identification occurs by means of estimating of the motor system reaction to a small precisely measured motor signal (test-impulse), which was generated simultaneously with the basic motor command outflow but reached the spinal motor structures more quickly. The mathematical description of the urgent identification principle performance in the motor control in the case of velocity estimating was suggested.", "contents": "[Use of the prompt identification principle in controlling complex motor systems (speed control)]. Some aspects of the adaptive control of animal motor system described in the form of differential equations system were discussed. It was considered that the system of motor control is a multilevel one in which each level has a definite range of correction capacity for movement deviations. Since the time of the sensory signal passing over the control nervous loop is comparable with the duration of the movement controlled, a mechanism of the fast adaptation to a nonstationary situation was suggested. This mechanism is based on the principle of urgent identification of the motor system state at the moment of movement initiation. Urgent identification occurs by means of estimating of the motor system reaction to a small precisely measured motor signal (test-impulse), which was generated simultaneously with the basic motor command outflow but reached the spinal motor structures more quickly. The mathematical description of the urgent identification principle performance in the motor control in the case of velocity estimating was suggested."}
{"id": "PMID:465563", "title": "[Effect of illumination prehistory on P700 redox transition kinetics in bean leaves].", "content": "The state I in equilibrium state II transients in bean leaves were studied by ESR method at various temperatures. P700 photooxidation induced by far-red light after leaf adaptation to darkness demonstrated two phases. This kinetics correlated with two overshuts in the kinetics of P700+ reduction observed after additional switching on the light exciting Photosystem II. In the atmosphere of gaseous nitrogen P700 behaviour was reversibly modified. Multiphase character of P700 kinetics correlated with the state I in equilibrium state II phenomenon as measured by chlorophyll a fluorescence from Photosystem II. The rate-limiting step at the acceptor side of Photosystem I electron transport chain may be considered as a point controlling the state of intact chloroplasts.", "contents": "[Effect of illumination prehistory on P700 redox transition kinetics in bean leaves]. The state I in equilibrium state II transients in bean leaves were studied by ESR method at various temperatures. P700 photooxidation induced by far-red light after leaf adaptation to darkness demonstrated two phases. This kinetics correlated with two overshuts in the kinetics of P700+ reduction observed after additional switching on the light exciting Photosystem II. In the atmosphere of gaseous nitrogen P700 behaviour was reversibly modified. Multiphase character of P700 kinetics correlated with the state I in equilibrium state II phenomenon as measured by chlorophyll a fluorescence from Photosystem II. The rate-limiting step at the acceptor side of Photosystem I electron transport chain may be considered as a point controlling the state of intact chloroplasts."}
{"id": "PMID:465569", "title": "Occurrence of an active regulatory mechanism of protein synthesis during starvation and refeeding in Bombyx mori fat body.", "content": "The origin of the amino acids which participate in protein synthesis at the recovery from starvation have been determined in the fat body from Bombyx mori larvae. Endogeneous amino acids have been labelled with [3H] leucine and ingested ones with [14C] leucine, allowing their discrimination in the organism. 22 minutes after refeeding, proteosynthetic activity of the fat body, estimated by the polysome level, is increased 2.5 fold. Endogeneous leucine represents more than 90 p. cent of the leucine present in nascent polypeptides. Free leucine pools of the fat body and of hemolymph increase, mainly through the release of endogeneous leucine. It is therefore concluded that refeeding with amino acids induces the production of a signal or critical factor, responsible for the increase in proteosynthetic activity in the fat body.", "contents": "Occurrence of an active regulatory mechanism of protein synthesis during starvation and refeeding in Bombyx mori fat body. The origin of the amino acids which participate in protein synthesis at the recovery from starvation have been determined in the fat body from Bombyx mori larvae. Endogeneous amino acids have been labelled with [3H] leucine and ingested ones with [14C] leucine, allowing their discrimination in the organism. 22 minutes after refeeding, proteosynthetic activity of the fat body, estimated by the polysome level, is increased 2.5 fold. Endogeneous leucine represents more than 90 p. cent of the leucine present in nascent polypeptides. Free leucine pools of the fat body and of hemolymph increase, mainly through the release of endogeneous leucine. It is therefore concluded that refeeding with amino acids induces the production of a signal or critical factor, responsible for the increase in proteosynthetic activity in the fat body."}
{"id": "PMID:465565", "title": "[Nonequilibrium distribution as a feature of biological systems].", "content": "It is suggested that the amount of energy extracted by a dissipative biological system from macroergic compounds depends on the energetic state of the system. The non-linear character of this relationship with the maximum in intermediate phases and presence of the upper limit of energetic states in the system with an energy increase cause a decrease of population of intermediate levels and an increase of the upper and lower levels. The antientropic distribution is presented as a principal thermodynamic peculiarity of biological systems.", "contents": "[Nonequilibrium distribution as a feature of biological systems]. It is suggested that the amount of energy extracted by a dissipative biological system from macroergic compounds depends on the energetic state of the system. The non-linear character of this relationship with the maximum in intermediate phases and presence of the upper limit of energetic states in the system with an energy increase cause a decrease of population of intermediate levels and an increase of the upper and lower levels. The antientropic distribution is presented as a principal thermodynamic peculiarity of biological systems."}
{"id": "PMID:465564", "title": "[Possible DNA conformations].", "content": "The complete analysis of possible DNA structures has been undertaken in the space of dihedral angles. The structural sets obtained join energetically and geometrically possible conformations. Thus, the necessary condtions of existence are fulfilled for these structural forms. The half of our structural families includes all structures and structural sets proposed by other authors [10, 24]. Other six families have not been presented elsewhere. The results of the energy optimization suggest the left handed forms are unfavourable energetically, because main chain torsional term and the energy of nucleotide moiety. At the same time there is a great number of various lefthanded forms.", "contents": "[Possible DNA conformations]. The complete analysis of possible DNA structures has been undertaken in the space of dihedral angles. The structural sets obtained join energetically and geometrically possible conformations. Thus, the necessary condtions of existence are fulfilled for these structural forms. The half of our structural families includes all structures and structural sets proposed by other authors [10, 24]. Other six families have not been presented elsewhere. The results of the energy optimization suggest the left handed forms are unfavourable energetically, because main chain torsional term and the energy of nucleotide moiety. At the same time there is a great number of various lefthanded forms."}
{"id": "PMID:465570", "title": "DNA synthesis and endomitoses in the giant nuclei of the silkgland of Bombyx mori.", "content": "At the first symptoms of organisation of the silkgland in the embryo, mitoses stop and nuclei start to grow. Through autoradiographic studies, performed after sequenced labeling with [3H] and [14C] thymidine, the durations of the different phases of DNA synthesis cycles (T = 42 to 48 h, S = 22 to 25 h, G = 20 to 23 h) are established. These durations are in fact identical during the second and the third instar, and the same, whatever region is concerned : posterior, middle or anterior parts. A model has been established to account for the distribution of the S phases during the second and third instars. The DNA synthesis in all nuclei of a given region has been followed during the first four instars by labelling with [3H]thymidine. The activity goes through maxima and minima, depending on the percent of nuclei synthesizing DNA and their synchronism, both being characteristic of each region; long resting periods are observed during the molting stages of the first three instars in the middle and the anterior parts. The coincidence is obvious between the maxima and minima and respectively the S and G phases of the model. DNA assays agree with the distribution of cycles established by autoradiography if one admits that each cycle does lead to a doubling of the amount of DNA. The overall DNA synthesis from the diploid value is estimated to correspond to 18-19 endomitoic cycles in the nucleic of the posterior part, 19-20 cycles of those of the middle part and 13 in those of the anterior part. The analysis of chromosome structures, by squashing the nuclear content, shows that existence of a complete endomitotic cycle: the doubling of chromosomes is associated with condensed structures, alternating with a decondensed state of chromatin, responsible for the DNA synthesis. The female heterochromatin undergoes a restricted morphological cycle delayed with respect to bulk chromatin. It is characterized by a late DNA duplication and by non dispersed daughter chromosomes. Some of these aspects are, to a lesser extent, reproduced in groups of autosomic chromosomes.", "contents": "DNA synthesis and endomitoses in the giant nuclei of the silkgland of Bombyx mori. At the first symptoms of organisation of the silkgland in the embryo, mitoses stop and nuclei start to grow. Through autoradiographic studies, performed after sequenced labeling with [3H] and [14C] thymidine, the durations of the different phases of DNA synthesis cycles (T = 42 to 48 h, S = 22 to 25 h, G = 20 to 23 h) are established. These durations are in fact identical during the second and the third instar, and the same, whatever region is concerned : posterior, middle or anterior parts. A model has been established to account for the distribution of the S phases during the second and third instars. The DNA synthesis in all nuclei of a given region has been followed during the first four instars by labelling with [3H]thymidine. The activity goes through maxima and minima, depending on the percent of nuclei synthesizing DNA and their synchronism, both being characteristic of each region; long resting periods are observed during the molting stages of the first three instars in the middle and the anterior parts. The coincidence is obvious between the maxima and minima and respectively the S and G phases of the model. DNA assays agree with the distribution of cycles established by autoradiography if one admits that each cycle does lead to a doubling of the amount of DNA. The overall DNA synthesis from the diploid value is estimated to correspond to 18-19 endomitoic cycles in the nucleic of the posterior part, 19-20 cycles of those of the middle part and 13 in those of the anterior part. The analysis of chromosome structures, by squashing the nuclear content, shows that existence of a complete endomitotic cycle: the doubling of chromosomes is associated with condensed structures, alternating with a decondensed state of chromatin, responsible for the DNA synthesis. The female heterochromatin undergoes a restricted morphological cycle delayed with respect to bulk chromatin. It is characterized by a late DNA duplication and by non dispersed daughter chromosomes. Some of these aspects are, to a lesser extent, reproduced in groups of autosomic chromosomes."}
{"id": "PMID:465571", "title": "The chemical structure and the crystalline structures of Bombyx mori silk fibroin.", "content": "Some recent data (i.e. published in the last ten years) on the chemical and crystalline structures of B. mori silk are reviewed. The main emphasis is put on the crystallizable portion of silk fibroin, including its chemical constitution and its molecular conformation (at the crystallographic unit-cell level) in the two crystalline modifications : the beta pleated sheet and the silk I structures. The structural aspects are based on a discussion of X-ray and electron diffraction data, and on conformational energy analyses of a model (Ala-Gly)n polypeptide of silk fibroin.", "contents": "The chemical structure and the crystalline structures of Bombyx mori silk fibroin. Some recent data (i.e. published in the last ten years) on the chemical and crystalline structures of B. mori silk are reviewed. The main emphasis is put on the crystallizable portion of silk fibroin, including its chemical constitution and its molecular conformation (at the crystallographic unit-cell level) in the two crystalline modifications : the beta pleated sheet and the silk I structures. The structural aspects are based on a discussion of X-ray and electron diffraction data, and on conformational energy analyses of a model (Ala-Gly)n polypeptide of silk fibroin."}
{"id": "PMID:465572", "title": "The adaptation of the silkgland cell to the production of fibroin in Bombyx mori L.", "content": "At the end of the larval life, the posterior silk gland of Bombyx mori is highly specialized in the biosynthesis of a specific protein : silk fibroin. The successive steps of fibroin production : amino supply, synthesis and secretion are described. Their analysis shows that fibroin synthesis is important enough to orient the overall cellular activities. Thus, the terminal differentiation of the posterior silk gland cell corresponds to the cells adaptation to the production of fibroin. Cytological and biochemical studies of the silk gland development show that specialization occurs discontinuously; the fourth molt, when fibroin is no more produced, is a phase of regression of the cellular adaptation whereas cell differentiation proceeds during the growth phase of the following fifth intermolt. After the spinning of the cocoon, the cells are lysed and disappear entirely at the nymphal stage. Biometrical analysis of silk production of different Bombyx strains in relation with the development of the proteosynthesis apparatus leads to the conclusion that the specific messenger RNA content determines the amount of synthetized fibroin. At maximum secretion, the mRNAF recruits almost all the cell ribosomes. The variations of the size of the proteosynthesis machinery are sufficient to explain the differences of productivity of the various silkworm strains. Different experimental factors affect silk production. Topical applications of juvenile hormone induce an increase of the RNA content and a consequent rise of the amount of secreted protein. In contrast, starvation reduces the silk production by acting at both transcriptonal and translational levels. Current researches on this system are devoted to the study of the differential gene expression, with particular interest to the regulation of the transcription of the specific fibroin messenger RNA.", "contents": "The adaptation of the silkgland cell to the production of fibroin in Bombyx mori L. At the end of the larval life, the posterior silk gland of Bombyx mori is highly specialized in the biosynthesis of a specific protein : silk fibroin. The successive steps of fibroin production : amino supply, synthesis and secretion are described. Their analysis shows that fibroin synthesis is important enough to orient the overall cellular activities. Thus, the terminal differentiation of the posterior silk gland cell corresponds to the cells adaptation to the production of fibroin. Cytological and biochemical studies of the silk gland development show that specialization occurs discontinuously; the fourth molt, when fibroin is no more produced, is a phase of regression of the cellular adaptation whereas cell differentiation proceeds during the growth phase of the following fifth intermolt. After the spinning of the cocoon, the cells are lysed and disappear entirely at the nymphal stage. Biometrical analysis of silk production of different Bombyx strains in relation with the development of the proteosynthesis apparatus leads to the conclusion that the specific messenger RNA content determines the amount of synthetized fibroin. At maximum secretion, the mRNAF recruits almost all the cell ribosomes. The variations of the size of the proteosynthesis machinery are sufficient to explain the differences of productivity of the various silkworm strains. Different experimental factors affect silk production. Topical applications of juvenile hormone induce an increase of the RNA content and a consequent rise of the amount of secreted protein. In contrast, starvation reduces the silk production by acting at both transcriptonal and translational levels. Current researches on this system are devoted to the study of the differential gene expression, with particular interest to the regulation of the transcription of the specific fibroin messenger RNA."}
{"id": "PMID:465573", "title": "Effect of starvation on tRNA synthesis, amino acid pool, tRNA charging levels and aminoacyl-tRNA synthetase activities in the posterior silk gland of Bombyx mori L.", "content": "Changes in the translational machinery components of the Bombyx mori posterior silk gland were analysed during starvation and refeeding and compared to the regularly fed larvae. During starvation, tRNA and ribosomal RNA synthesis are stopped. The amounts of different RNA classes and of the different tRNA species slow down at the same rate. Thus various tRNA show similar half-lifes and the preexisting tRNA adaptation to fibroin mRNA translation persists during starvation. Similarly, the tRNA/rRNA ratio is constant during starvation and refeeding (12 tRNA molecules for one ribosome) as in silk glands of control animals. Aminoacyl-tRNA synthetases and tRNA charging levels are decreased during starvation. The maximal tRNA charging level obtained during maximal protein synthesis in control animals is regained after 24 h refeeding of starved larvae. Changes observed in the free amino acid pool are not similar from one amino acid to another and levels reached after starvation do not differ strongly from the controls. Our results suggest that the production of translation apparatus components is coordinated and adjusted to the protein synthesis activity. Whether this coordination occurs in the silk gland is discussed on the basis of the \"metabolic regulation\", primarily described in prokaryotes and Yeast. Transfer RNA charging levels seem to play a key role in the process of regulation and could be implicated in the mechanism of tRNA adaptation if this phenomenon results as expected from a transcriptional control.", "contents": "Effect of starvation on tRNA synthesis, amino acid pool, tRNA charging levels and aminoacyl-tRNA synthetase activities in the posterior silk gland of Bombyx mori L. Changes in the translational machinery components of the Bombyx mori posterior silk gland were analysed during starvation and refeeding and compared to the regularly fed larvae. During starvation, tRNA and ribosomal RNA synthesis are stopped. The amounts of different RNA classes and of the different tRNA species slow down at the same rate. Thus various tRNA show similar half-lifes and the preexisting tRNA adaptation to fibroin mRNA translation persists during starvation. Similarly, the tRNA/rRNA ratio is constant during starvation and refeeding (12 tRNA molecules for one ribosome) as in silk glands of control animals. Aminoacyl-tRNA synthetases and tRNA charging levels are decreased during starvation. The maximal tRNA charging level obtained during maximal protein synthesis in control animals is regained after 24 h refeeding of starved larvae. Changes observed in the free amino acid pool are not similar from one amino acid to another and levels reached after starvation do not differ strongly from the controls. Our results suggest that the production of translation apparatus components is coordinated and adjusted to the protein synthesis activity. Whether this coordination occurs in the silk gland is discussed on the basis of the \"metabolic regulation\", primarily described in prokaryotes and Yeast. Transfer RNA charging levels seem to play a key role in the process of regulation and could be implicated in the mechanism of tRNA adaptation if this phenomenon results as expected from a transcriptional control."}
{"id": "PMID:465574", "title": "Studies on tRNA adaptation, tRNA turnover, precursor tRNA and tRNA gene distribution in Bombyx mori by using two-dimensional polyacrylamide gel electrophoresis.", "content": "Eighteen out of twenty amino acids have been used for identifying tRNAs from the silkworm Bombyx mori L. fractionated on two-dimensional polyacrylamide gel electrophoresis. 43 spots out of 53 have been identified. This mapping confirms previous results and brings new answers to some questions on the regulation of tRNA biosynthesis. 1. In addition to quantitative adaptation of tRNAs to the composition of silk proteins (fibroin from the posterior silk gland, sericin from the middle part) and of iso-tRNAs from posterior silk gland to the major codons of fibroin mRNA, we also observe adaptation of tRNA from various tissues to the average amino acid content of proteins from fat body, gut, gonads and carcass of the silkworm. 2. In the silk gland, turnover rates of several tRNA species are similar. The selective accumulation of tRNAs needed for decoding fibroin and sericin mRNAs which takes place during the Vth larval instar, cannot be explained by the occurrence of a preferential degradation of some tRNA species. 3. Under given conditions for incubating silk glands, it is possible to obtain an accumulation of precursor tRNA species, which are enriched in pre-tRNAAla and pre-tRNAGly in the posterior silk gland and pre-tRNASer in the middle part. 4. The distribution of tRNA genes is not random. tRNA genes for glycine, alanine and serine are prominent. Selective transcription of batteries of iso-tRNA genes could explain our data.", "contents": "Studies on tRNA adaptation, tRNA turnover, precursor tRNA and tRNA gene distribution in Bombyx mori by using two-dimensional polyacrylamide gel electrophoresis. Eighteen out of twenty amino acids have been used for identifying tRNAs from the silkworm Bombyx mori L. fractionated on two-dimensional polyacrylamide gel electrophoresis. 43 spots out of 53 have been identified. This mapping confirms previous results and brings new answers to some questions on the regulation of tRNA biosynthesis. 1. In addition to quantitative adaptation of tRNAs to the composition of silk proteins (fibroin from the posterior silk gland, sericin from the middle part) and of iso-tRNAs from posterior silk gland to the major codons of fibroin mRNA, we also observe adaptation of tRNA from various tissues to the average amino acid content of proteins from fat body, gut, gonads and carcass of the silkworm. 2. In the silk gland, turnover rates of several tRNA species are similar. The selective accumulation of tRNAs needed for decoding fibroin and sericin mRNAs which takes place during the Vth larval instar, cannot be explained by the occurrence of a preferential degradation of some tRNA species. 3. Under given conditions for incubating silk glands, it is possible to obtain an accumulation of precursor tRNA species, which are enriched in pre-tRNAAla and pre-tRNAGly in the posterior silk gland and pre-tRNASer in the middle part. 4. The distribution of tRNA genes is not random. tRNA genes for glycine, alanine and serine are prominent. Selective transcription of batteries of iso-tRNA genes could explain our data."}
{"id": "PMID:465575", "title": "Dynamics of macromolecular populations: a mathematical model of the quantitative changes of RNA in the silkgland during the last larval instar.", "content": "The quantitative changes of RNA in the silkgland of Bombyx mori have been studied during the last larval instar by using a mathematical model (Volterra-Kostitzin model). This model can be associated with a global mechanism including synthesis and degradative processes. The numerical and statistical methods used for model analysis are described in an appendix. Thus we have compared the accumulation of total RNA (essentially ribosomal) after a treatment (juvenile hormone) and between several strains. The importance of the degradative factor is denoted to explain the observed differences, whereas the synthesis rates remain relatively stable. The last observation may lead us to an interpretation of the molecular effect of a selection to increase silk production : rather than an increase of the productivity of cellular machinery, the degradative process has been limited.", "contents": "Dynamics of macromolecular populations: a mathematical model of the quantitative changes of RNA in the silkgland during the last larval instar. The quantitative changes of RNA in the silkgland of Bombyx mori have been studied during the last larval instar by using a mathematical model (Volterra-Kostitzin model). This model can be associated with a global mechanism including synthesis and degradative processes. The numerical and statistical methods used for model analysis are described in an appendix. Thus we have compared the accumulation of total RNA (essentially ribosomal) after a treatment (juvenile hormone) and between several strains. The importance of the degradative factor is denoted to explain the observed differences, whereas the synthesis rates remain relatively stable. The last observation may lead us to an interpretation of the molecular effect of a selection to increase silk production : rather than an increase of the productivity of cellular machinery, the degradative process has been limited."}
{"id": "PMID:465576", "title": "Juvenile hormone modifies larvae and silk gland development in Bombyx mori.", "content": "Topical application of a Juvenile Hormone Analogue (ZR 515) during the first half of the first half of the last (fifth) larval instar of Bombyx mori induces a prolongation of this instar and increases larvae weight and silk secretion. Later treatment can lead to an extra larval molt. Moreover, the hormone analogue stops either the development or the secretory activity of the silk gland, depending on the period of treatments. When Juvenile Hormone is administered at the beginning of the fifth instar, silk gland growth as well as RNA and protein syntheses are first inhibited and later resumed and amplified. Finaly silk production increases up to 150 per cent of controls. One of the primarly effects of treatments seems to be RNA synthesis. Thus Juvenile Hormone analogue application could be a new tool for studying  RNA transcription and/or processing.", "contents": "Juvenile hormone modifies larvae and silk gland development in Bombyx mori. Topical application of a Juvenile Hormone Analogue (ZR 515) during the first half of the first half of the last (fifth) larval instar of Bombyx mori induces a prolongation of this instar and increases larvae weight and silk secretion. Later treatment can lead to an extra larval molt. Moreover, the hormone analogue stops either the development or the secretory activity of the silk gland, depending on the period of treatments. When Juvenile Hormone is administered at the beginning of the fifth instar, silk gland growth as well as RNA and protein syntheses are first inhibited and later resumed and amplified. Finaly silk production increases up to 150 per cent of controls. One of the primarly effects of treatments seems to be RNA synthesis. Thus Juvenile Hormone analogue application could be a new tool for studying  RNA transcription and/or processing."}
{"id": "PMID:465579", "title": "Effects of contingent versus yoked temperature feedback on voluntary temperature control and cold stress tolerance.", "content": "Twenty-four male internals (locus of control) and 24 externals were instructed to increase finger temperature under one of three conditions: (1) contingent feedback (CF), (2) yoked sham feedback (YF), or (3) no feedback (NF). Five 13-min training sessions were given. Feedback was then removed and subjects tested for voluntary temperature control. Finally, the cold pressor test, a laboratory analogue of natural cold stress, was administered under no-feedback conditions. Results demonstrated voluntary control of peripheral temperature following contingent feedback training, but not after yoked feedback temperature training. Contrary to expectation, the acquisition of voluntary control did not attenuate the stress response to thermal pain. Differences between internals and externals throughout the study were generally insignificant.", "contents": "Effects of contingent versus yoked temperature feedback on voluntary temperature control and cold stress tolerance. Twenty-four male internals (locus of control) and 24 externals were instructed to increase finger temperature under one of three conditions: (1) contingent feedback (CF), (2) yoked sham feedback (YF), or (3) no feedback (NF). Five 13-min training sessions were given. Feedback was then removed and subjects tested for voluntary temperature control. Finally, the cold pressor test, a laboratory analogue of natural cold stress, was administered under no-feedback conditions. Results demonstrated voluntary control of peripheral temperature following contingent feedback training, but not after yoked feedback temperature training. Contrary to expectation, the acquisition of voluntary control did not attenuate the stress response to thermal pain. Differences between internals and externals throughout the study were generally insignificant."}
{"id": "PMID:465578", "title": "Heart rate and blood pressure biofeedback: II. A review and integration of recent theoretical models.", "content": "The theoretical models put forth separately by Bremer, Lang, and Schwartz to account for the data on biofeedback and cardiovascular responses are summarized and evaluated in light of recent empirical studies. Although each model makes some use of the idea that learning to control heart rate, particularly heart rate acceleration, is like motor skills learning, each model also emphasizes different sets of factors or variable. An attempted integration of these three models is presented.", "contents": "Heart rate and blood pressure biofeedback: II. A review and integration of recent theoretical models. The theoretical models put forth separately by Bremer, Lang, and Schwartz to account for the data on biofeedback and cardiovascular responses are summarized and evaluated in light of recent empirical studies. Although each model makes some use of the idea that learning to control heart rate, particularly heart rate acceleration, is like motor skills learning, each model also emphasizes different sets of factors or variable. An attempted integration of these three models is presented."}
{"id": "PMID:465580", "title": "The effects of EMG feedback training during problem solving: a case study.", "content": "The present case study investigated the effects of competing task demands on biofeedback training to reduce frontalis muscle tension. Baseline levels of frontalis muscle tension were recorded for relaxation and problem solving. The subject was trained to decrease muscle tension with biofeedback for the problem-solving task alone. The results indicated that EMG training during problem-solving was successfully accomplished. Frontalis muscle tension during relaxation baseline did not change as a result of reductions in muscle tension during problem-solving feedback training. This suggests that the decrease of muscle tension cannot be attributed to reductions in overall muscle tension levels. Instead, training was specific to the problem-solving feedback phases. Additionally, it was found that accuracy in problem-solving did not decline as a result of simultaneous feedback training. Thus EMG biofeedback training can be accomplished and exercised without disruption of ongoing mental activity.", "contents": "The effects of EMG feedback training during problem solving: a case study. The present case study investigated the effects of competing task demands on biofeedback training to reduce frontalis muscle tension. Baseline levels of frontalis muscle tension were recorded for relaxation and problem solving. The subject was trained to decrease muscle tension with biofeedback for the problem-solving task alone. The results indicated that EMG training during problem-solving was successfully accomplished. Frontalis muscle tension during relaxation baseline did not change as a result of reductions in muscle tension during problem-solving feedback training. This suggests that the decrease of muscle tension cannot be attributed to reductions in overall muscle tension levels. Instead, training was specific to the problem-solving feedback phases. Additionally, it was found that accuracy in problem-solving did not decline as a result of simultaneous feedback training. Thus EMG biofeedback training can be accomplished and exercised without disruption of ongoing mental activity."}
{"id": "PMID:465581", "title": "Target training: a technique for assessing self-regulation of skin temperature.", "content": "The determination of the achievement of self-regulation of hand temperature can be difficult, even with adequate baseline and control procedures. Although bidirectional training is often mentioned as a technique for thorough learning and for a convincing demonstration of temperature control, patients are sometimes unwilling or unable to lower hand temperature volitionally. This case report describes a patient who, due to failure at lowering her hand temperature, developed the target training method to convince herself of her self-regulation skill. The method may be useful in both clinical and research settings.", "contents": "Target training: a technique for assessing self-regulation of skin temperature. The determination of the achievement of self-regulation of hand temperature can be difficult, even with adequate baseline and control procedures. Although bidirectional training is often mentioned as a technique for thorough learning and for a convincing demonstration of temperature control, patients are sometimes unwilling or unable to lower hand temperature volitionally. This case report describes a patient who, due to failure at lowering her hand temperature, developed the target training method to convince herself of her self-regulation skill. The method may be useful in both clinical and research settings."}
{"id": "PMID:465582", "title": "Evaluation and statistical judgement of neural responses to sinusoidal stimulation in cases with superimposed drift and noise.", "content": "Time histograms of neural responses evoked by sinuosidal stimulation often contain a slow drifting and an irregular noise which disturb Fourier analysis of these responses. Section 2 of this paper evaluates the extent to which a linear drift influences the Fourier analysis, and develops a combined Fourier and linear regression analysis for detecting and correcting for such a linear drift. Usefulness of this correcting method is demonstrated for the time histograms of actual eye movements and Purkinje cell discharges evoked by sinusoidal rotation of rabbits in the horizontal plane. In Sect. 3, the analysis of variance is adopted for estimating the probability of the random occurrence of the response curve extracted by Fourier analysis from noise. This method proved to be useful for avoiding false judgements as to whether the response curve was meaningful, particularly when the response was small relative to the contaminating noise.", "contents": "Evaluation and statistical judgement of neural responses to sinusoidal stimulation in cases with superimposed drift and noise. Time histograms of neural responses evoked by sinuosidal stimulation often contain a slow drifting and an irregular noise which disturb Fourier analysis of these responses. Section 2 of this paper evaluates the extent to which a linear drift influences the Fourier analysis, and develops a combined Fourier and linear regression analysis for detecting and correcting for such a linear drift. Usefulness of this correcting method is demonstrated for the time histograms of actual eye movements and Purkinje cell discharges evoked by sinusoidal rotation of rabbits in the horizontal plane. In Sect. 3, the analysis of variance is adopted for estimating the probability of the random occurrence of the response curve extracted by Fourier analysis from noise. This method proved to be useful for avoiding false judgements as to whether the response curve was meaningful, particularly when the response was small relative to the contaminating noise."}
{"id": "PMID:465583", "title": "Some notes on the statistical properties of a posteriori Wiener filtering.", "content": "In this study the authors analyse the asymptotic distribution of estimated a posteriori Wiener filtering of averaged evoked responses and the problem of the existence and convergence of moments of the estimated filter and the filtered signal. The authors concluded that the only justification of a posteriori Wiener filtering lies in the possibility to use it as a vehicle of a priori information.", "contents": "Some notes on the statistical properties of a posteriori Wiener filtering. In this study the authors analyse the asymptotic distribution of estimated a posteriori Wiener filtering of averaged evoked responses and the problem of the existence and convergence of moments of the estimated filter and the filtered signal. The authors concluded that the only justification of a posteriori Wiener filtering lies in the possibility to use it as a vehicle of a priori information."}
{"id": "PMID:465584", "title": "Entrainment of oscillatory neural activity in the cat's lateral geniculate nucleus.", "content": "Oscillatory neural activity in the frequency range 7-12 Hz is observed in the lateral geniculate nucleus (LGN) of the lightly anesthetized cat. This paper describes a series of experiments in which the interactions between ongoing oscillatory potentials and periodic photic and electrical stimuli are analyzed using frequency domain techniques. The principal results of these experiments are consistent with a model of the neural system as an entrainable oscillator in which ongoing oscillations are suppressed by stimulation at nearby frequencies, but coexist with stimulus frequencies farther away. The physiological interpretation of these results may be closely tied to the role of the LGN as a \"gating\" mechanism between retina and cortex.", "contents": "Entrainment of oscillatory neural activity in the cat's lateral geniculate nucleus. Oscillatory neural activity in the frequency range 7-12 Hz is observed in the lateral geniculate nucleus (LGN) of the lightly anesthetized cat. This paper describes a series of experiments in which the interactions between ongoing oscillatory potentials and periodic photic and electrical stimuli are analyzed using frequency domain techniques. The principal results of these experiments are consistent with a model of the neural system as an entrainable oscillator in which ongoing oscillations are suppressed by stimulation at nearby frequencies, but coexist with stimulus frequencies farther away. The physiological interpretation of these results may be closely tied to the role of the LGN as a \"gating\" mechanism between retina and cortex."}
{"id": "PMID:465586", "title": "A volume conductor study of compound action potentials of nerves in situ: the forward problem.", "content": "A computer model for the stimulation of compound nerve action potentials, based on superposition of volume conducted single nerve fibre potentials, is presented. The model assumes that the intracellular fibre potential, the fibre diameter distribution and the electrical conductivities of different tissues are known. Volume conductor fields are calculated in the spatial frequency domain. The influence of important parameters in the model is evaluated numerically. It is shown that it is necessary to give up the usual assumption of homogeneity and isotropy in the extracellular medium. In the present model parameters are introduced which allow an overall description of the complex morphological and physiological structure of the nerve trunk. Simulation results indicate that the model is a rather promising tool in studying the main properties of compound action potentials which up till now have not been sufficiently well understood.", "contents": "A volume conductor study of compound action potentials of nerves in situ: the forward problem. A computer model for the stimulation of compound nerve action potentials, based on superposition of volume conducted single nerve fibre potentials, is presented. The model assumes that the intracellular fibre potential, the fibre diameter distribution and the electrical conductivities of different tissues are known. Volume conductor fields are calculated in the spatial frequency domain. The influence of important parameters in the model is evaluated numerically. It is shown that it is necessary to give up the usual assumption of homogeneity and isotropy in the extracellular medium. In the present model parameters are introduced which allow an overall description of the complex morphological and physiological structure of the nerve trunk. Simulation results indicate that the model is a rather promising tool in studying the main properties of compound action potentials which up till now have not been sufficiently well understood."}
{"id": "PMID:465587", "title": "[Activity, isoenzyme composition, thermostability and molecular weight of peroxidase from intact and virus infected tobacco plant leaves].", "content": "The enzyme peroxidase was isolated from the leaves of the tobacco plant Xanthi (intact and infected with weakly (XY) and highly (XT) pathogenic strains of potato X-virus) and partially purified. The original extract (the 30,000 g supernatant) was purified by ammonium sulfate at 30--80% of saturation and by gel filtration through Sephadex G-25 and G-100 in 0.05 M tris-HCl buffer, pH 7.4 containing 17% sucrose. Disc electrophoresis revealed that both intact and infected plants contain 10 isoperoxidases. The electrophoregrams of isoenzymes from infected plants with the Rf values of 0.1, 0.48, 0.53 and 0.59 stained with benzidine produced a more intensive colouring as compared to the corresponding isoenzymes from intact plants. The total enzymatic activity for the plants infected with the XY and XT strains made up to 180% and 240% of that for the intact plants, respectively. The molecular weights of the peroxidase isoenzymes were found to be the same and equal to 40,000. Study of the thermostability at 60 degrees C and pH 7.0 showed that after 90 min the enzyme activity was 12.4% and 5.1% of the original one in intact and infected plants, respectively. The data obtained suggest that the activity, thermostability and synthesis of some peroxidase isoenzymes in tobacco plant leaves are affected by viral infection.", "contents": "[Activity, isoenzyme composition, thermostability and molecular weight of peroxidase from intact and virus infected tobacco plant leaves]. The enzyme peroxidase was isolated from the leaves of the tobacco plant Xanthi (intact and infected with weakly (XY) and highly (XT) pathogenic strains of potato X-virus) and partially purified. The original extract (the 30,000 g supernatant) was purified by ammonium sulfate at 30--80% of saturation and by gel filtration through Sephadex G-25 and G-100 in 0.05 M tris-HCl buffer, pH 7.4 containing 17% sucrose. Disc electrophoresis revealed that both intact and infected plants contain 10 isoperoxidases. The electrophoregrams of isoenzymes from infected plants with the Rf values of 0.1, 0.48, 0.53 and 0.59 stained with benzidine produced a more intensive colouring as compared to the corresponding isoenzymes from intact plants. The total enzymatic activity for the plants infected with the XY and XT strains made up to 180% and 240% of that for the intact plants, respectively. The molecular weights of the peroxidase isoenzymes were found to be the same and equal to 40,000. Study of the thermostability at 60 degrees C and pH 7.0 showed that after 90 min the enzyme activity was 12.4% and 5.1% of the original one in intact and infected plants, respectively. The data obtained suggest that the activity, thermostability and synthesis of some peroxidase isoenzymes in tobacco plant leaves are affected by viral infection."}
{"id": "PMID:465588", "title": "[Specificity of intracellular ribonucleases Pc1 and Pc2 of the fungus Penicillium claviforme].", "content": "The products of RNA and synthetic polynucleotides degradation by intracellular RNAses Pc1 and Pc2 of the fungus Penicillium claviforme were studied. It was shown that the enzymes possess the endonuclease activity and are not specific for the bases vicinal to the cleaved PDE bonds (EC 3.1.4.23). The increase of binding of the dinucleoside monophosphates by Pc1 and Pc2 dependent on the nucleoside at the 3'-end of the PDE bond is: A greater than C greater than G greater than U. This order is opposite for the rates of these substrates cleavage by the RNAses. A homologous specificity of the intracellular RNAse Pc1 and the extracellular RNAse II of Pen. claviforme has been revealed.", "contents": "[Specificity of intracellular ribonucleases Pc1 and Pc2 of the fungus Penicillium claviforme]. The products of RNA and synthetic polynucleotides degradation by intracellular RNAses Pc1 and Pc2 of the fungus Penicillium claviforme were studied. It was shown that the enzymes possess the endonuclease activity and are not specific for the bases vicinal to the cleaved PDE bonds (EC 3.1.4.23). The increase of binding of the dinucleoside monophosphates by Pc1 and Pc2 dependent on the nucleoside at the 3'-end of the PDE bond is: A greater than C greater than G greater than U. This order is opposite for the rates of these substrates cleavage by the RNAses. A homologous specificity of the intracellular RNAse Pc1 and the extracellular RNAse II of Pen. claviforme has been revealed."}
{"id": "PMID:465589", "title": "[Thermal stability of lactate dehydrogenase and alcohol dehydrogenase incorporated into highly concentrated gels].", "content": "The rate constants for inactivation of lactate dehydrogenase and alcohol dehydrogenase in solution at 65 degrees C (pH 7,5) are 0,72 and 0,013 min-1, respectively. The enzyme incorporation into acrylamide gels results in immobilized enzymes, whose residual activity is 18--25% of the original one. In 6,7% gels the rate of thermal inactivation for lactate dehydrogenase is decreased nearly 10-fold, whereas the inactivation rate for alcohol dehydrogenase is increased 4,6-fold as compared to the soluble enzymes. In 14% and 40% gels the inactivation constants for lactate dehydrogenase are 6,3.10(-3) and 5,9.10(-4) min-1, respectively. In 60% gels the thermal inactivation of lactate dehydrogenase is decelerated 3600-fold as compared to the native enzyme. The enthalpy and enthropy for the inactivation of the native enzyme are equal to 62,8 kcal/mole and 116,9 cal/(mole.grad.) for the native enzyme and those of gel-incorporated (6,7%) enzyme -- 38,7 kcal/mole and 42 cal/(mole.grad.), respectively. The thermal stability of alcohol dehydrogenase in 60% gels is increased 12-fold. To prevent gel swelling, methacrylic acid and allylamine were added to the matrix, with subsequent treatment by dicyclohexylcarbodiimide. The enzyme activity of the modified gels is 2,7--3% of that for the 6,7% gels. The stability of lactate dehydrogenase in such gels is significantly increased. A mechanism of stabilization of the subunit enzymes in highly concentrated gels is discussed.", "contents": "[Thermal stability of lactate dehydrogenase and alcohol dehydrogenase incorporated into highly concentrated gels]. The rate constants for inactivation of lactate dehydrogenase and alcohol dehydrogenase in solution at 65 degrees C (pH 7,5) are 0,72 and 0,013 min-1, respectively. The enzyme incorporation into acrylamide gels results in immobilized enzymes, whose residual activity is 18--25% of the original one. In 6,7% gels the rate of thermal inactivation for lactate dehydrogenase is decreased nearly 10-fold, whereas the inactivation rate for alcohol dehydrogenase is increased 4,6-fold as compared to the soluble enzymes. In 14% and 40% gels the inactivation constants for lactate dehydrogenase are 6,3.10(-3) and 5,9.10(-4) min-1, respectively. In 60% gels the thermal inactivation of lactate dehydrogenase is decelerated 3600-fold as compared to the native enzyme. The enthalpy and enthropy for the inactivation of the native enzyme are equal to 62,8 kcal/mole and 116,9 cal/(mole.grad.) for the native enzyme and those of gel-incorporated (6,7%) enzyme -- 38,7 kcal/mole and 42 cal/(mole.grad.), respectively. The thermal stability of alcohol dehydrogenase in 60% gels is increased 12-fold. To prevent gel swelling, methacrylic acid and allylamine were added to the matrix, with subsequent treatment by dicyclohexylcarbodiimide. The enzyme activity of the modified gels is 2,7--3% of that for the 6,7% gels. The stability of lactate dehydrogenase in such gels is significantly increased. A mechanism of stabilization of the subunit enzymes in highly concentrated gels is discussed."}
{"id": "PMID:465590", "title": "[Denaturation of beef liver glutamate dehydrogenase under the action of guanidine hydrochloride and a study of the possibility of the enzyme renaturation].", "content": "It was shown that denaturation of beef liver glutamate dehydrogenase under the action of guanidine hydrochloride results in a diplacement of the protein fluorescence maximum from 332 to 349 nm, in a decrease of optical rotation of the protein at 233 nm and in an appearance of negative bands in the difference absorbance spectrum with extrema at 279 and 287 nm. The transition of native enzyme into a denaturated state is observed within a narrow interval of guanidine hydrochloride concentrations. The middle point of the transition corresponds to approximately 2,2 M guanidine hydrochloride. The inactivation kinetics for glutamate dehydrogenase coincide with those of the enzyme spectral properties alterations due to denaturation. The attempts at renaturation of glutamate dehydrogenase by diluting the denaturated enzyme solution or by a dialysis against a buffer solution were unsuccessful.", "contents": "[Denaturation of beef liver glutamate dehydrogenase under the action of guanidine hydrochloride and a study of the possibility of the enzyme renaturation]. It was shown that denaturation of beef liver glutamate dehydrogenase under the action of guanidine hydrochloride results in a diplacement of the protein fluorescence maximum from 332 to 349 nm, in a decrease of optical rotation of the protein at 233 nm and in an appearance of negative bands in the difference absorbance spectrum with extrema at 279 and 287 nm. The transition of native enzyme into a denaturated state is observed within a narrow interval of guanidine hydrochloride concentrations. The middle point of the transition corresponds to approximately 2,2 M guanidine hydrochloride. The inactivation kinetics for glutamate dehydrogenase coincide with those of the enzyme spectral properties alterations due to denaturation. The attempts at renaturation of glutamate dehydrogenase by diluting the denaturated enzyme solution or by a dialysis against a buffer solution were unsuccessful."}
{"id": "PMID:465591", "title": "[Interaction of substrates and their analogs with adenosylcobalamine-dependent glycerol dehydratase].", "content": "The interaction of AdoCbl-dependent glycerol dehydratase with the substrates (glycerol, 1,2-propandiol, ethylene glycol) and their analogs (aliphatic diols) was studied kinetically. It was found that all the diols tested are competitive inhibitors of the enzyme with respect to substrates. The arrangement of hydroxyl groups in the molecule, the length of the carbohydrate chain and the nature of the substituent at the C-3 atom are essential for the binding of diol in the active center. The ternary enzyme-AdoCbl-substrate (analog) complexes are subjected to specific inactivation at a rate, which depends on the chemical structure of the substrate (analog). The constants for inactivation and dissociation of the ternary complexes were determined. It was shown that in contrast to the double complexes (enzyme-AdoCbl), the inactivation of the ternary complexes does not depend on oxygen. Some aspects of the mechanism of specific inactivation of glycerol dehydratase are discussed.", "contents": "[Interaction of substrates and their analogs with adenosylcobalamine-dependent glycerol dehydratase]. The interaction of AdoCbl-dependent glycerol dehydratase with the substrates (glycerol, 1,2-propandiol, ethylene glycol) and their analogs (aliphatic diols) was studied kinetically. It was found that all the diols tested are competitive inhibitors of the enzyme with respect to substrates. The arrangement of hydroxyl groups in the molecule, the length of the carbohydrate chain and the nature of the substituent at the C-3 atom are essential for the binding of diol in the active center. The ternary enzyme-AdoCbl-substrate (analog) complexes are subjected to specific inactivation at a rate, which depends on the chemical structure of the substrate (analog). The constants for inactivation and dissociation of the ternary complexes were determined. It was shown that in contrast to the double complexes (enzyme-AdoCbl), the inactivation of the ternary complexes does not depend on oxygen. Some aspects of the mechanism of specific inactivation of glycerol dehydratase are discussed."}
{"id": "PMID:465592", "title": "[Effect of dibromothymoquinone on photosynthetic electron transport].", "content": "The effect of dibromothymoquinone on photosynthetic electron transport in pea dependent on concentration was studied. Dibromothymoquinone inhibited general electron transport from water to NADP+ in isolated chloroplasts and ethiochloroplasts and the electron transfer via plastoquinone and cytochrome f in the leaves and isolated plastids. At all concentrations studied dibromothymoquinone significantly affected the absorption changes at 590 nm in the ethiochloroplasts associated with plastocyanine photoreactions. Possible location of electron carriers in the photosynthetic electron transport chain is discussed.", "contents": "[Effect of dibromothymoquinone on photosynthetic electron transport]. The effect of dibromothymoquinone on photosynthetic electron transport in pea dependent on concentration was studied. Dibromothymoquinone inhibited general electron transport from water to NADP+ in isolated chloroplasts and ethiochloroplasts and the electron transfer via plastoquinone and cytochrome f in the leaves and isolated plastids. At all concentrations studied dibromothymoquinone significantly affected the absorption changes at 590 nm in the ethiochloroplasts associated with plastocyanine photoreactions. Possible location of electron carriers in the photosynthetic electron transport chain is discussed."}
{"id": "PMID:465593", "title": "[Participation of oxygen activated species in enzymatic lipid peroxidation in biomembranes].", "content": "The participation of oxygen activated species in the induction of lipid peroxidation (LPO) in the membrane systems containing cytochrome P-450 (liver microsomes) and in the membrane fragments devoid of this hemoprotein (brain and skeletal muscle microsomes) was studied. It was shown that the rate of NADH-dependent LPO does not depend on the presence of hemoproteins and the activity of NADH-specific flavoprotein in the membranes. On the other hand, the microsomal membranes of the liver with high specific contents of b5 and P-450 cytochromes and NADPH-specific flavoprotein, had the highest rates of NADPH-dependent LPO. It was found that the most effective inhibitors of free oxygen activated species in the case of NADPH- and NADH-dependent LPO in the microsomal fractions of liver, brain and skeletal muscles are the superoxide (O ./2) anion radical inhibitors. The singlet oxygen (1O2) quenchers inhibit only NADPH-dependent LPO in the liver, however, in a far lesser degree. The hydroxyl radical (OH) scavengers had no effect on enzymatic LPO in all systems studied.", "contents": "[Participation of oxygen activated species in enzymatic lipid peroxidation in biomembranes]. The participation of oxygen activated species in the induction of lipid peroxidation (LPO) in the membrane systems containing cytochrome P-450 (liver microsomes) and in the membrane fragments devoid of this hemoprotein (brain and skeletal muscle microsomes) was studied. It was shown that the rate of NADH-dependent LPO does not depend on the presence of hemoproteins and the activity of NADH-specific flavoprotein in the membranes. On the other hand, the microsomal membranes of the liver with high specific contents of b5 and P-450 cytochromes and NADPH-specific flavoprotein, had the highest rates of NADPH-dependent LPO. It was found that the most effective inhibitors of free oxygen activated species in the case of NADPH- and NADH-dependent LPO in the microsomal fractions of liver, brain and skeletal muscles are the superoxide (O ./2) anion radical inhibitors. The singlet oxygen (1O2) quenchers inhibit only NADPH-dependent LPO in the liver, however, in a far lesser degree. The hydroxyl radical (OH) scavengers had no effect on enzymatic LPO in all systems studied."}
{"id": "PMID:465594", "title": "[Reconstitution of liver endoplasmic reticulum membranes from solubilized proteins and lipids by removal of detergent].", "content": "A method for membrane reconstitution from cholate-solubilized microsomal proteins and lipids by a removal of the detergent on a column with charcoal has been developed. A comparative study showed that the membranes reconstituted by a dialysis or absorption do not differ from each other in terms of membrane proteins incorporation into lipid vesicles and cytochrome P-450 reconversion into cytochrome P-450. A possibility of biomembrane reconstitution from membrane proteins and lipids solubilized by a non-ionic detergent Triton X-100 was shown. A removal of the detergent results in a formation of membranes, which are chemically close to the original ones but ultrastructurally very different from the latter. On the other hand, absorption or dialysis of cholate-solubilized proteins and lipids results in reconstituted membranes with asymmetrically arranged intramembrane particles located on the hydrophobic surfaces of the membrane halves. The number and size of these particles are similar to those of the original microsomal membranes.", "contents": "[Reconstitution of liver endoplasmic reticulum membranes from solubilized proteins and lipids by removal of detergent]. A method for membrane reconstitution from cholate-solubilized microsomal proteins and lipids by a removal of the detergent on a column with charcoal has been developed. A comparative study showed that the membranes reconstituted by a dialysis or absorption do not differ from each other in terms of membrane proteins incorporation into lipid vesicles and cytochrome P-450 reconversion into cytochrome P-450. A possibility of biomembrane reconstitution from membrane proteins and lipids solubilized by a non-ionic detergent Triton X-100 was shown. A removal of the detergent results in a formation of membranes, which are chemically close to the original ones but ultrastructurally very different from the latter. On the other hand, absorption or dialysis of cholate-solubilized proteins and lipids results in reconstituted membranes with asymmetrically arranged intramembrane particles located on the hydrophobic surfaces of the membrane halves. The number and size of these particles are similar to those of the original microsomal membranes."}
{"id": "PMID:465595", "title": "[Proteinase activity of nuclei from various tissues towards endogenous histones].", "content": "The proteinase activities of nuclei isolated from tissues differing in their mitotic activities (brain, thymus, liver, ascite lymphoma) towards the histones and non-histone acid -- extractable proteins were studied. The sensitivity of different histone fractions to nuclear proteinase depends on temperature and time of nuclei incubation under conditions providing for complete dissociation of chromatin proteins from DNA (2 M NaCl--5 M urea). The proteinase activity in the brain and thymus nuclei is revealed only under prolonged (43 hrs) incubation of the nuclei at 25 degrees C, which is accompanied by partial proteolysis of histone H1. Histone H4 from brain nuclei and histone H2a from thymus nuclei are preferably degraded. In the nuclei isolated from the mice ascite cell lymphoma NK/ly and from rat liver the enzyme activity is revealed mainly towards the arginine-enriched histones H3 and H4. The proteolysis of the arginine-enriched histones in tumour cell nuclei is more complete. A high sensitivity to proteolysis was observed for non-histone acid-extractable proteins with low electrophoretic mobility, which were found in brain and tumour cell nuclei.", "contents": "[Proteinase activity of nuclei from various tissues towards endogenous histones]. The proteinase activities of nuclei isolated from tissues differing in their mitotic activities (brain, thymus, liver, ascite lymphoma) towards the histones and non-histone acid -- extractable proteins were studied. The sensitivity of different histone fractions to nuclear proteinase depends on temperature and time of nuclei incubation under conditions providing for complete dissociation of chromatin proteins from DNA (2 M NaCl--5 M urea). The proteinase activity in the brain and thymus nuclei is revealed only under prolonged (43 hrs) incubation of the nuclei at 25 degrees C, which is accompanied by partial proteolysis of histone H1. Histone H4 from brain nuclei and histone H2a from thymus nuclei are preferably degraded. In the nuclei isolated from the mice ascite cell lymphoma NK/ly and from rat liver the enzyme activity is revealed mainly towards the arginine-enriched histones H3 and H4. The proteolysis of the arginine-enriched histones in tumour cell nuclei is more complete. A high sensitivity to proteolysis was observed for non-histone acid-extractable proteins with low electrophoretic mobility, which were found in brain and tumour cell nuclei."}
{"id": "PMID:465596", "title": "[Circular dichroism spectra of protein proteinase inhibitors].", "content": "The circular dichroism spectra of two protein proteinase inhibitors were studied. The CD spectrum of the kidney bean inhibitor is similar to those of other low molecular weight inhibitors from legume seeds. The potato inhibitor in its native state is characterized by a low content of alpha-helices, which is increased in the presence of sodium dodecyl sulfate.", "contents": "[Circular dichroism spectra of protein proteinase inhibitors]. The circular dichroism spectra of two protein proteinase inhibitors were studied. The CD spectrum of the kidney bean inhibitor is similar to those of other low molecular weight inhibitors from legume seeds. The potato inhibitor in its native state is characterized by a low content of alpha-helices, which is increased in the presence of sodium dodecyl sulfate."}
{"id": "PMID:465597", "title": "[Properties of pig liver transketolase].", "content": "Some properties of homogeneous transketolase from pig liver were studied. It was shown that the pH optimum of the transketolase reaction lies within the range of 7.8--8.2. The isoelectric point is at pH 7.6--7.8. The molecular weight of transketolase is 138,000 +/- 3,000 as determined by the sedimentation equilibrium method and about 152,000 according to the data from gel filtration through Sephadex G-200. The enzyme molecule is a tetramer of the alpha 2 beta 2 type. The molecular weights of the alpha- and beta- subunits determined by polyacrylamide gel in the presence of sodium dodecyl sulfate are 52,000--56,000 and 27,000--29,000, respectively. Transketolase contains about two moles of TPP per mole of protein and does not require metal ions for its catalytic activity.", "contents": "[Properties of pig liver transketolase]. Some properties of homogeneous transketolase from pig liver were studied. It was shown that the pH optimum of the transketolase reaction lies within the range of 7.8--8.2. The isoelectric point is at pH 7.6--7.8. The molecular weight of transketolase is 138,000 +/- 3,000 as determined by the sedimentation equilibrium method and about 152,000 according to the data from gel filtration through Sephadex G-200. The enzyme molecule is a tetramer of the alpha 2 beta 2 type. The molecular weights of the alpha- and beta- subunits determined by polyacrylamide gel in the presence of sodium dodecyl sulfate are 52,000--56,000 and 27,000--29,000, respectively. Transketolase contains about two moles of TPP per mole of protein and does not require metal ions for its catalytic activity."}
{"id": "PMID:465598", "title": "[Interaction of rat liver dexamethasone-receptor complexes with DNA].", "content": "Rat liver glucocorticoid-receptor complexes (GRC) acquire the ability to bind to DNA in a high affinity manner after activation by heating or precipitation with (NH)2SO4. DNA is practically non-saturable by GRC in low salt buffers as well as in 0.15 M NaCl-containing buffer, although in the latter case the binding decreases approximately 3--5 times. GRC bind to homo- and heterologous prokaryotic DNA in a similar way; in both cases an addition of KCl (up to 0.15 M) to the medium is followed by the same decrease of the binding. This data suggest that the association of GRC with DNA observed in vitro is not accompanied by \"recognition\" of any certain DNA site. Besides DNA, activated GRC can associate with other polymers, charged positively (DEAE-cellulose) or negatively (RNA, polyvinylsulfate). GRC interact very weakly with neutral compounds of the cellulose type but are strongly adsorbed on hydroxyapatite. Hence the activated GRC can be considered as an amphoteric protein. Salt solutions provoke dissociation of the GRC-DNA triple complexes: a complete dissociation is observed in the presence of 0,4 M NaCl or 0,4 M sodium phosphate buffer (pH 6,9). Sodium phosphate buffer also elutes GRC from other sorbents such as DEAE-cellulose or hydroxyapatite. No significant dissociation of the GRC-DNA complexes is observed at sucrose concentration up to 2 M. The data obtained are indicative of an essential role of electrostatic forces for the interaction of GRC with DNA. The non-ionic detergent Triton X-100 at a concentration as low as 0,05% completely destroys the GRC-DNA triple complexes. The models explicating the  selectivity of the genome activation by GRC without their \"recognition\" of any specific DNA sequences are proposed.", "contents": "[Interaction of rat liver dexamethasone-receptor complexes with DNA]. Rat liver glucocorticoid-receptor complexes (GRC) acquire the ability to bind to DNA in a high affinity manner after activation by heating or precipitation with (NH)2SO4. DNA is practically non-saturable by GRC in low salt buffers as well as in 0.15 M NaCl-containing buffer, although in the latter case the binding decreases approximately 3--5 times. GRC bind to homo- and heterologous prokaryotic DNA in a similar way; in both cases an addition of KCl (up to 0.15 M) to the medium is followed by the same decrease of the binding. This data suggest that the association of GRC with DNA observed in vitro is not accompanied by \"recognition\" of any certain DNA site. Besides DNA, activated GRC can associate with other polymers, charged positively (DEAE-cellulose) or negatively (RNA, polyvinylsulfate). GRC interact very weakly with neutral compounds of the cellulose type but are strongly adsorbed on hydroxyapatite. Hence the activated GRC can be considered as an amphoteric protein. Salt solutions provoke dissociation of the GRC-DNA triple complexes: a complete dissociation is observed in the presence of 0,4 M NaCl or 0,4 M sodium phosphate buffer (pH 6,9). Sodium phosphate buffer also elutes GRC from other sorbents such as DEAE-cellulose or hydroxyapatite. No significant dissociation of the GRC-DNA complexes is observed at sucrose concentration up to 2 M. The data obtained are indicative of an essential role of electrostatic forces for the interaction of GRC with DNA. The non-ionic detergent Triton X-100 at a concentration as low as 0,05% completely destroys the GRC-DNA triple complexes. The models explicating the  selectivity of the genome activation by GRC without their \"recognition\" of any specific DNA sequences are proposed."}
{"id": "PMID:465599", "title": "[Analysis of brain chromatin subunit composition using different endonucleases].", "content": "A comparison of the processes of chromatin digestion in brain and liver nuclei by Ca, Mg-dependent and staphylococcal endonucleases demonstrates a similarity of the subunit composition of chromatin from both tissues and reveals the same type of linked DNA regions. However, a formation of low molecular weight DNP fragments during hydrolysis and the DNA spectra of soluble and insoluble DNP fragments suggest that brain chromatin contains these fragments alongside with the regions, which are specific for this particular tissue, predominate in it and are resistant to staphylococcal and, particularly, to Ca, Mg-dependent endonucleases. This is paralleled with a non-histone protein enrichment of different brain chromatin fractions and an expansion of the electrophoretic monomer band towards the fragment with a greater molecular weight. It may be assumed that brain nucleosomes are characterized by a higher size heterogeneity of linked DNA, part of which are mostly covered by non-histone proteins, and/or are characterized by a greater set variety.", "contents": "[Analysis of brain chromatin subunit composition using different endonucleases]. A comparison of the processes of chromatin digestion in brain and liver nuclei by Ca, Mg-dependent and staphylococcal endonucleases demonstrates a similarity of the subunit composition of chromatin from both tissues and reveals the same type of linked DNA regions. However, a formation of low molecular weight DNP fragments during hydrolysis and the DNA spectra of soluble and insoluble DNP fragments suggest that brain chromatin contains these fragments alongside with the regions, which are specific for this particular tissue, predominate in it and are resistant to staphylococcal and, particularly, to Ca, Mg-dependent endonucleases. This is paralleled with a non-histone protein enrichment of different brain chromatin fractions and an expansion of the electrophoretic monomer band towards the fragment with a greater molecular weight. It may be assumed that brain nucleosomes are characterized by a higher size heterogeneity of linked DNA, part of which are mostly covered by non-histone proteins, and/or are characterized by a greater set variety."}
{"id": "PMID:465600", "title": "[Isolation and purification of bovine and porcine cerebral cathepsin D].", "content": "Cathepsin D was isolated from the grey matter of bovine and porcine large cerebral hemispheres and purified by affinity chromatography on haemoglobin--Sepharose. The isolation and purification of the enzyme also included: acidic extraction, precipitation by ammonium sulfate, dialysis, affinity chromatography, concentration and gel-chromatography on Sephadex G-100. The degree of purification of bovine cerebral enzyme was 3280. The Km value for the enzyme was 2,06 . 10(-5) M. The purified enzyme from bovine brain showed three major and two minor adjacent bands, possessing the cathepsin D activities. The purified enzyme from porcine brain showed only one protein band.", "contents": "[Isolation and purification of bovine and porcine cerebral cathepsin D]. Cathepsin D was isolated from the grey matter of bovine and porcine large cerebral hemispheres and purified by affinity chromatography on haemoglobin--Sepharose. The isolation and purification of the enzyme also included: acidic extraction, precipitation by ammonium sulfate, dialysis, affinity chromatography, concentration and gel-chromatography on Sephadex G-100. The degree of purification of bovine cerebral enzyme was 3280. The Km value for the enzyme was 2,06 . 10(-5) M. The purified enzyme from bovine brain showed three major and two minor adjacent bands, possessing the cathepsin D activities. The purified enzyme from porcine brain showed only one protein band."}
{"id": "PMID:465601", "title": "[Biochemical analysis of RNA particles and nucleolar RNases in eucaryotic cells].", "content": "Four types of rat liver nucleolar RNP particles with the sedimentation coefficients of 120S, 80S, 60S and 12--16S were analyzed and their chemical composition was established. The nucleolar RNP particles were found to contain a RNA set with the sedimentation coefficients of 43S, 33S, 20S and 7S. The nucleolar proteins were shown to contain endonucleases, one of which was isolated and partially purified by fractionation on DEAE-Sephadex A-50. In some of its properties this RNAse was found similar to an analogous enzyme from membrane-bound ribosomes.", "contents": "[Biochemical analysis of RNA particles and nucleolar RNases in eucaryotic cells]. Four types of rat liver nucleolar RNP particles with the sedimentation coefficients of 120S, 80S, 60S and 12--16S were analyzed and their chemical composition was established. The nucleolar RNP particles were found to contain a RNA set with the sedimentation coefficients of 43S, 33S, 20S and 7S. The nucleolar proteins were shown to contain endonucleases, one of which was isolated and partially purified by fractionation on DEAE-Sephadex A-50. In some of its properties this RNAse was found similar to an analogous enzyme from membrane-bound ribosomes."}
{"id": "PMID:465602", "title": "[Isolation and properties of cytochrome P-450 from rabbit liver microsomes].", "content": "A purified low-spin form of cytochrome P-450 was isolated from phenobarbital-induced rabbit liver microsomes. The preparation was functionally active and free from cytochromes b5 and P-420 and phospholipids. The specific content of the cytochrome was 18 nmoles per mg of protein. At the molecular weight of the hemoprotein of 50,000, it corresponds to 90% of purification. The purified hemoprotein binds substrates of type II and some substrates of type I. The complexes formed reveal spectral properties, similar to those for the complexes of these substrates with the microsomal form of cytochrome P-450.", "contents": "[Isolation and properties of cytochrome P-450 from rabbit liver microsomes]. A purified low-spin form of cytochrome P-450 was isolated from phenobarbital-induced rabbit liver microsomes. The preparation was functionally active and free from cytochromes b5 and P-420 and phospholipids. The specific content of the cytochrome was 18 nmoles per mg of protein. At the molecular weight of the hemoprotein of 50,000, it corresponds to 90% of purification. The purified hemoprotein binds substrates of type II and some substrates of type I. The complexes formed reveal spectral properties, similar to those for the complexes of these substrates with the microsomal form of cytochrome P-450."}
{"id": "PMID:465603", "title": "[Methylation and degradation of chromatin DNA in isolated rat liver cell nuclei].", "content": "Methylation of chromatin DNA in rat liver cell nuclei incubated in a medium with [3H]CH3-S-adenosyl methionine was studied. It was shown that under the given experimental conditions DNA methylation and chromatin degradation by endogenous nuclear nuclease (nucleases) with a formation of chromatin structural subunits occur simultaneously. An analysis of methylated chromatin DNA degradation products based on a number of approaches demonstrated a predominant methylation of extra-nucleosomal DNA. The data obtained suggest that chromatin of isolated nuclei contain sites with supermethylated DNA fragments incorporating not less than 400 nucleotide pairs. These sites possess an increased sensitivity to endogenous nuclease.", "contents": "[Methylation and degradation of chromatin DNA in isolated rat liver cell nuclei]. Methylation of chromatin DNA in rat liver cell nuclei incubated in a medium with [3H]CH3-S-adenosyl methionine was studied. It was shown that under the given experimental conditions DNA methylation and chromatin degradation by endogenous nuclear nuclease (nucleases) with a formation of chromatin structural subunits occur simultaneously. An analysis of methylated chromatin DNA degradation products based on a number of approaches demonstrated a predominant methylation of extra-nucleosomal DNA. The data obtained suggest that chromatin of isolated nuclei contain sites with supermethylated DNA fragments incorporating not less than 400 nucleotide pairs. These sites possess an increased sensitivity to endogenous nuclease."}
{"id": "PMID:465604", "title": "[Comparative study of cytoplasmic RNA-binding proteins of eukaryotic cells: content and polypeptide composition].", "content": "The specific RNA-binding activity of cytoplasmic extracts of a number of eukaryotic cells and tissues was determined by sorption of radioactive RNA on nitrocellulose filters. This activity varies within a wide range: from 0,5 up to 80 microgram of 23S-RNA per mg of extract protein. The percentage of RNA-binding proteins as measured by their adsorption on RNA-Sepharose is 0,3--60% of total protein of cytoplasmic extracts and is well-correlated with their RNA-binding activity. The variability of the RNA-binding activity of the isolated preparations of RNA-binding proteins (from 100 to 300 microgram of 23S-RNA per mg of protein) is indicative of their qualitative differences. The analysis of the polypeptide composition of RNA-binding proteins revealed that the set of the major polypeptide chains is rather simple (with few exceptions) and includes both universal components with mol. weights of about 36,000, 49,000 and 95,000 characteristic of a number of cells and tissues and tissue- and species-specific polypeptides. Upon differentiation of rabbit reticulocytes into erythrocytes the percentage of RNA-binding proteins is decreased 4-fold and one of the three main polypeptides with the mol. weight of about 95,000 disappears. After fecundation of sea urchin ovicelles the polypeptide with the mol. weight of 37,000 disappears from the preparations of RNA-binding proteins and at the morula stage two new components with the mol. weights of about 35,000 and 26,000 appear instead of it. The germination of wheat embryos results in a two-fold increase of the percentage of RNA-binding proteins without any essential changes of the set of main polypeptide chains.", "contents": "[Comparative study of cytoplasmic RNA-binding proteins of eukaryotic cells: content and polypeptide composition]. The specific RNA-binding activity of cytoplasmic extracts of a number of eukaryotic cells and tissues was determined by sorption of radioactive RNA on nitrocellulose filters. This activity varies within a wide range: from 0,5 up to 80 microgram of 23S-RNA per mg of extract protein. The percentage of RNA-binding proteins as measured by their adsorption on RNA-Sepharose is 0,3--60% of total protein of cytoplasmic extracts and is well-correlated with their RNA-binding activity. The variability of the RNA-binding activity of the isolated preparations of RNA-binding proteins (from 100 to 300 microgram of 23S-RNA per mg of protein) is indicative of their qualitative differences. The analysis of the polypeptide composition of RNA-binding proteins revealed that the set of the major polypeptide chains is rather simple (with few exceptions) and includes both universal components with mol. weights of about 36,000, 49,000 and 95,000 characteristic of a number of cells and tissues and tissue- and species-specific polypeptides. Upon differentiation of rabbit reticulocytes into erythrocytes the percentage of RNA-binding proteins is decreased 4-fold and one of the three main polypeptides with the mol. weight of about 95,000 disappears. After fecundation of sea urchin ovicelles the polypeptide with the mol. weight of 37,000 disappears from the preparations of RNA-binding proteins and at the morula stage two new components with the mol. weights of about 35,000 and 26,000 appear instead of it. The germination of wheat embryos results in a two-fold increase of the percentage of RNA-binding proteins without any essential changes of the set of main polypeptide chains."}
{"id": "PMID:465605", "title": "[Properties of DNA-dependent RNA-polymerase from spleens of mice infected with Rauscher leukemic virus].", "content": "The properties of RNA polymerases A, B and C isolated from the spleens of mice infected with Rauscher leukemic virus were studied. The solubilized RNA-polymerases A and B were purified 150--300-fold. The dynamic changes in the activities of all forms of RNA-polymerases at different stages of leukosis were studied. At the earliest steps of leukosis a 2-fold increase in the RNA-polymerase B activity followed by a 5-fold increase in the RNA-polymerase A activity was observed. At late stages of leukosis the activity of RNA-polymerase C also showed an increase. The properties of RNA-polymerases A and B from the spleens of virus-infected mice were compared to those of the controls. In leukemic tissues the specific activities of RNA-polymerases A and B were higher as compared to those of the enzymes isolated from the spleens of non-infected mice. However, no significant differences in the enzyme properties in normal and virus-infected animals were revealed. Dihydrorifampicine (200 mkg/ml) caused a 50% inhibition of RNA polymerase A in vitro but had no effect on the activities of RNA-polymerases B and C.", "contents": "[Properties of DNA-dependent RNA-polymerase from spleens of mice infected with Rauscher leukemic virus]. The properties of RNA polymerases A, B and C isolated from the spleens of mice infected with Rauscher leukemic virus were studied. The solubilized RNA-polymerases A and B were purified 150--300-fold. The dynamic changes in the activities of all forms of RNA-polymerases at different stages of leukosis were studied. At the earliest steps of leukosis a 2-fold increase in the RNA-polymerase B activity followed by a 5-fold increase in the RNA-polymerase A activity was observed. At late stages of leukosis the activity of RNA-polymerase C also showed an increase. The properties of RNA-polymerases A and B from the spleens of virus-infected mice were compared to those of the controls. In leukemic tissues the specific activities of RNA-polymerases A and B were higher as compared to those of the enzymes isolated from the spleens of non-infected mice. However, no significant differences in the enzyme properties in normal and virus-infected animals were revealed. Dihydrorifampicine (200 mkg/ml) caused a 50% inhibition of RNA polymerase A in vitro but had no effect on the activities of RNA-polymerases B and C."}
{"id": "PMID:465606", "title": "[Biogenesis of chromatin in animal cells. Stimulation of nuclear protein and DNA synthesis in hepatocytes after brief inhibition of translation by cycloheximide].", "content": "A drastic and brief inhibition of protein synthesis (up to 95% for 3--6 hrs) by cycloheximide in the liver of rats starved for 24 hrs results in a recovery and subsequent marked stimulation of non-histone proteins, histone chromosomal proteins and DNA. The stimulation of non-histone protein synthesis was observed after 1 hr (inhibition) 12--24 hrs (recovery and stimulation of protein synthesis) and 48--60 hrs (stimulation of DNA synthesis) following the administration of cycloheximide. Two periods of histone biosynthesis were observed. The first one (24--36 hrs) was not coupled and the second one (48--60 hrs) was coupled with DNA replication. During the recovery and stimulation of protein synthesis acetylation of the histone and non-histone proteins proceeds at an increased rate. Possible applicability of the model in question for investigations of chromatin biogenesis is discussed.", "contents": "[Biogenesis of chromatin in animal cells. Stimulation of nuclear protein and DNA synthesis in hepatocytes after brief inhibition of translation by cycloheximide]. A drastic and brief inhibition of protein synthesis (up to 95% for 3--6 hrs) by cycloheximide in the liver of rats starved for 24 hrs results in a recovery and subsequent marked stimulation of non-histone proteins, histone chromosomal proteins and DNA. The stimulation of non-histone protein synthesis was observed after 1 hr (inhibition) 12--24 hrs (recovery and stimulation of protein synthesis) and 48--60 hrs (stimulation of DNA synthesis) following the administration of cycloheximide. Two periods of histone biosynthesis were observed. The first one (24--36 hrs) was not coupled and the second one (48--60 hrs) was coupled with DNA replication. During the recovery and stimulation of protein synthesis acetylation of the histone and non-histone proteins proceeds at an increased rate. Possible applicability of the model in question for investigations of chromatin biogenesis is discussed."}
{"id": "PMID:465607", "title": "[Nuclease from Aspergillus oryzae, specific for single-stranded regions of nucleic acids].", "content": "A single-stranded specific nuclease has been purified from amyloryzine obtained from the mould fungi Aspergillus cryzae. The nuclease under study resembles the enzymes described in the literature in its ability to hydrolyze single-stranded nucleic acids. However, the enzyme essentially differs from previously known nucleases in some catalytic properties, particularly in its ability for degradation of poly A. It has been shown that the enzyme also hydrolyzes the synthetic dinucleotide pTpT to mononucleoside phosphates.", "contents": "[Nuclease from Aspergillus oryzae, specific for single-stranded regions of nucleic acids]. A single-stranded specific nuclease has been purified from amyloryzine obtained from the mould fungi Aspergillus cryzae. The nuclease under study resembles the enzymes described in the literature in its ability to hydrolyze single-stranded nucleic acids. However, the enzyme essentially differs from previously known nucleases in some catalytic properties, particularly in its ability for degradation of poly A. It has been shown that the enzyme also hydrolyzes the synthetic dinucleotide pTpT to mononucleoside phosphates."}
{"id": "PMID:465608", "title": "[Isolation and some properties of ATP-dependent DNAse from sea urchin (Strongylocentrotus intermedius) embryo].", "content": "An ATP-dependent DNAse was isolated from the cells of the sea urchin Strongylocentrotus intermedius embryos by chromatography on DEAE-cellulose and gel chromatography on Sepharose 4B. The enzyme was found homogeneous during polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined by gel filtration through Sepharose 6B and was equal to approximately 450,000. The sedimentation coefficient as determined by ultracentrifugation was equal to approximately 15S. The pH optimum during native DNA hydrolysis lies within the pH range of 6,5--9,0 and that during hydrolysis of denaturated DNA--within the pH range of 9,0--9,5. The enzyme was activated by ATP and dATP at the optimal concentration of 10(-4) M. Other nucleoside triphosphates did not substitute for ATP in this reaction. The hydrolysis of denaturated DNA occurred via the exonuclease way with a formation of short (di-, tri,- tetra- and penta-) oligonucleotides. The enzyme hydrolyzed native DNA according to the endonuclease type with predominant formation of high molecular weight polynucleotides.", "contents": "[Isolation and some properties of ATP-dependent DNAse from sea urchin (Strongylocentrotus intermedius) embryo]. An ATP-dependent DNAse was isolated from the cells of the sea urchin Strongylocentrotus intermedius embryos by chromatography on DEAE-cellulose and gel chromatography on Sepharose 4B. The enzyme was found homogeneous during polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined by gel filtration through Sepharose 6B and was equal to approximately 450,000. The sedimentation coefficient as determined by ultracentrifugation was equal to approximately 15S. The pH optimum during native DNA hydrolysis lies within the pH range of 6,5--9,0 and that during hydrolysis of denaturated DNA--within the pH range of 9,0--9,5. The enzyme was activated by ATP and dATP at the optimal concentration of 10(-4) M. Other nucleoside triphosphates did not substitute for ATP in this reaction. The hydrolysis of denaturated DNA occurred via the exonuclease way with a formation of short (di-, tri,- tetra- and penta-) oligonucleotides. The enzyme hydrolyzed native DNA according to the endonuclease type with predominant formation of high molecular weight polynucleotides."}
{"id": "PMID:465618", "title": "Three experiments on the effects of information frequency and feedback timing on instructed heart rate speeding.", "content": "Three experiments are reported comparing different biofeedback displays in a heart rate speeding task. The first experiment examined the effects of heart rate feedback presented at three frequencies in a fixed-time format. Information was given at 0.5-sec, 2-sec and 8-sec intervals. Results indicated that both the 0.5-sec and 8-sec groups' speeding performances were superior to that of the 2-sec group. The second experiment compared a 1-sec fixed-time group with groups receiving displays in which feedback was presented synchronously with systole. Feedback was synchronized either with every heart beat or every tenth beat. The one-beat group was superior to both the 1-sec and 10-beat groups. Experiment III again presented displays which terminated with every beat or every tenth beat. However, in the previous experiment heart interval information was presented only briefly at the systole ending the sample period. In Experiment III, criterion terminations remained on the feedback screen throughout the subsequent interval. Thus, subjects did not have the additional task of attending to very briefly presented information. Nevertheless, speeding performances of the one-beat group were again superior to that attained by the 10-beat group. In all experiments a relationship between increased respiratory and skin conductance levels and heart rate speeding performances was found, suggesting that heart rate speeding was part of a generalized pattern of arousal. It was concluded that instructed heart rate speeding is highly sensitive to changes both in the frequency of feedback presentation, and to the type of display (fixed-time or heart-time) presented.", "contents": "Three experiments on the effects of information frequency and feedback timing on instructed heart rate speeding. Three experiments are reported comparing different biofeedback displays in a heart rate speeding task. The first experiment examined the effects of heart rate feedback presented at three frequencies in a fixed-time format. Information was given at 0.5-sec, 2-sec and 8-sec intervals. Results indicated that both the 0.5-sec and 8-sec groups' speeding performances were superior to that of the 2-sec group. The second experiment compared a 1-sec fixed-time group with groups receiving displays in which feedback was presented synchronously with systole. Feedback was synchronized either with every heart beat or every tenth beat. The one-beat group was superior to both the 1-sec and 10-beat groups. Experiment III again presented displays which terminated with every beat or every tenth beat. However, in the previous experiment heart interval information was presented only briefly at the systole ending the sample period. In Experiment III, criterion terminations remained on the feedback screen throughout the subsequent interval. Thus, subjects did not have the additional task of attending to very briefly presented information. Nevertheless, speeding performances of the one-beat group were again superior to that attained by the 10-beat group. In all experiments a relationship between increased respiratory and skin conductance levels and heart rate speeding performances was found, suggesting that heart rate speeding was part of a generalized pattern of arousal. It was concluded that instructed heart rate speeding is highly sensitive to changes both in the frequency of feedback presentation, and to the type of display (fixed-time or heart-time) presented."}
{"id": "PMID:465619", "title": "Interactions between heart rate, psychomotor performance and perceived effort during physical work as influenced by beta-adrenergic blockade.", "content": "Effects of a single intravenous dose of propranolol (0,25 mg/kg body weight) were examined in 15 healthy male subjects who performed three reaction-time tasks of different complexity, while pedalling at five work loads on a cycle ergometer. Comparisons between measurements after propranolol and after injection of a placebo solution showed a pronounced reduction of heart rate and an increase in catecholamine excretion following propranolol. Comparisons of psychomotor performance showed no significan difference between the propranolol and placebo conditions. Nor did self-estimates of perceived physical and task-induced efforts reveal any significant effects of propranolol. The results support the notion that heart rate is not a prominent cue for perceived effort.", "contents": "Interactions between heart rate, psychomotor performance and perceived effort during physical work as influenced by beta-adrenergic blockade. Effects of a single intravenous dose of propranolol (0,25 mg/kg body weight) were examined in 15 healthy male subjects who performed three reaction-time tasks of different complexity, while pedalling at five work loads on a cycle ergometer. Comparisons between measurements after propranolol and after injection of a placebo solution showed a pronounced reduction of heart rate and an increase in catecholamine excretion following propranolol. Comparisons of psychomotor performance showed no significan difference between the propranolol and placebo conditions. Nor did self-estimates of perceived physical and task-induced efforts reveal any significant effects of propranolol. The results support the notion that heart rate is not a prominent cue for perceived effort."}
{"id": "PMID:465620", "title": "Evoked potential correlates of human information processing.", "content": "The late positive component (P3) of the feedback evoked potential was investigated in two tasks: the detection of near-threshold auditory stimuli, and the estimation of 1 s time intervals. When the intensity or probability of the threshold stimuli was varied in the detection task, it became apparent that the feedback P3 was related to the 'contingent probability' of confirming or disconfirming feedback given a particular response. In the time-estimation task the relative probabiliti-s of confirming and disconfirming feedback were altered by changing the time-window wherein a response was judged correct. In these experiments it was found that the feedback P3 component was highly dependent upon the probability of the feedback, and relatively independent of its confirming or disconfirming meaning. With decreasing probability of the feedback stimuli, the P3 component became larger, later, and somewhat more frontal.", "contents": "Evoked potential correlates of human information processing. The late positive component (P3) of the feedback evoked potential was investigated in two tasks: the detection of near-threshold auditory stimuli, and the estimation of 1 s time intervals. When the intensity or probability of the threshold stimuli was varied in the detection task, it became apparent that the feedback P3 was related to the 'contingent probability' of confirming or disconfirming feedback given a particular response. In the time-estimation task the relative probabiliti-s of confirming and disconfirming feedback were altered by changing the time-window wherein a response was judged correct. In these experiments it was found that the feedback P3 component was highly dependent upon the probability of the feedback, and relatively independent of its confirming or disconfirming meaning. With decreasing probability of the feedback stimuli, the P3 component became larger, later, and somewhat more frontal."}
{"id": "PMID:465621", "title": "Sleep and stressor exposure in relation to circadian rhythms in catecholamine excretion.", "content": "12 healthy male volunteers spent 64 h of continuous waking under strictly controlled environmental conditions (light, food, drink, activity) in isolation from the external world. Before and after the vigil the subjects slept in the laboratory. An additional group of five participated only during day time and spent the intervening night period asleep at home. Measurements were carried out in 3 h intervals except for sleep periods. Shortly before the circadian trough and peak of adrenaline excretion respectively, the subjects were exposed to a performance stressor. Results from the vigil showed a very pronounced circadian rhythm for adrenaline excretion but none for noradrenaline excretion. For adrenaline, night-time sleep reduced excretion levels, causing an even more pronounced circadian pattern. For noradrenaline, night-time sleep caused a drop in excretion giving the impression of a circadian rhythm. These and previous results led to the conclusion that the excretion of adrenaline exhibits a self-sustained rhythm while a rhythm in noradrenaline excretion is found only when caused by external synchronizers such as sleep-wake alternation. No difference in magnitude of stress response between peak and trough was observed for any of the catecholamines. Night-time (trough) exposure completely obliterated the circadian rhythm of adrenaline excretion for the duration of the exposure. It was concluded that the normal pronounced night-time trough of adrenaline cannot be due to unavailability of adrenaline in the medulla. With respect to sleep deprivation, no effect was found on excretion levels during waking, during sleep, or in response to the stressor as deprivation progressed. Finally, there was also found to be a close temporal covariation between adrenaline excretion and both rectal temperature and self-rated fatigue (neg.).", "contents": "Sleep and stressor exposure in relation to circadian rhythms in catecholamine excretion. 12 healthy male volunteers spent 64 h of continuous waking under strictly controlled environmental conditions (light, food, drink, activity) in isolation from the external world. Before and after the vigil the subjects slept in the laboratory. An additional group of five participated only during day time and spent the intervening night period asleep at home. Measurements were carried out in 3 h intervals except for sleep periods. Shortly before the circadian trough and peak of adrenaline excretion respectively, the subjects were exposed to a performance stressor. Results from the vigil showed a very pronounced circadian rhythm for adrenaline excretion but none for noradrenaline excretion. For adrenaline, night-time sleep reduced excretion levels, causing an even more pronounced circadian pattern. For noradrenaline, night-time sleep caused a drop in excretion giving the impression of a circadian rhythm. These and previous results led to the conclusion that the excretion of adrenaline exhibits a self-sustained rhythm while a rhythm in noradrenaline excretion is found only when caused by external synchronizers such as sleep-wake alternation. No difference in magnitude of stress response between peak and trough was observed for any of the catecholamines. Night-time (trough) exposure completely obliterated the circadian rhythm of adrenaline excretion for the duration of the exposure. It was concluded that the normal pronounced night-time trough of adrenaline cannot be due to unavailability of adrenaline in the medulla. With respect to sleep deprivation, no effect was found on excretion levels during waking, during sleep, or in response to the stressor as deprivation progressed. Finally, there was also found to be a close temporal covariation between adrenaline excretion and both rectal temperature and self-rated fatigue (neg.)."}
{"id": "PMID:465622", "title": "Voluntary heart rate control: the role of individual differences.", "content": "It is a frequent observation that individuals differ markedly in their success at voluntary heart rate control. Several dispositional variables have now received attention as possible sources of such individual variation. These are critically reviewed under the following headings: resting and stimulus-elicited heart rate characteristics; autonomic and cardiac perception; imagery vividness; locus of control; trait anxiety. It is concluded that the research on individual differences in cardiac control to date is contributing to a clearer understanding of the mechanisms underlying control. However, for this contribution to be sustained, research needs to service explicit hypotheses about mediating processes. The often arbitrary and atheoretical practices that characterize much of the research in the more general context of individual differences in physiological activity must be avoided.", "contents": "Voluntary heart rate control: the role of individual differences. It is a frequent observation that individuals differ markedly in their success at voluntary heart rate control. Several dispositional variables have now received attention as possible sources of such individual variation. These are critically reviewed under the following headings: resting and stimulus-elicited heart rate characteristics; autonomic and cardiac perception; imagery vividness; locus of control; trait anxiety. It is concluded that the research on individual differences in cardiac control to date is contributing to a clearer understanding of the mechanisms underlying control. However, for this contribution to be sustained, research needs to service explicit hypotheses about mediating processes. The often arbitrary and atheoretical practices that characterize much of the research in the more general context of individual differences in physiological activity must be avoided."}
{"id": "PMID:465623", "title": "Early selective-attention effects on the evoked potential: a critical review and reinterpretation.", "content": "Recent research on the effect of selective attention on the N1, component of the evoked potential is reviewed. These studies are based on the finding of Hillyard Hink, Schwent and Picton (1973) that this component is selectively enhanced in response to attended stimuli when a very rapid rate of stimulus delivery is used. On the basis of the subsequent set of experiments, the conditions and limits of the existence of the auditory 'N1 effect' are now quite clear. Moreover, this finding has been extended to somatosensory and visual modalities. In the present review a detailed examination of these studies has suggested a re-interpretation of the N1 effect. According to this reinterpretation, it is not a 'true' N1 component which is enhanced but the effect is produced by a summation of a negative shift with the evoked-potential wave form. Under some conditions such as those involving a very fast rate of stimulus delivery, this effect commences very early, making the N1 component appear larger. It is suggested that this shift reflects orienting to, and further processing of, an input found relevant in a preliminary sensory analysis. Topographical evidence for this kind of interpretation is provided by several studies. This negative shift is, hence, associated with voluntary attention. Some of the reviewed studies have described a rather similar negative shift as a correlate of involuntary attention to rare stimuli among the much more frequent, 'standard', stimuli.", "contents": "Early selective-attention effects on the evoked potential: a critical review and reinterpretation. Recent research on the effect of selective attention on the N1, component of the evoked potential is reviewed. These studies are based on the finding of Hillyard Hink, Schwent and Picton (1973) that this component is selectively enhanced in response to attended stimuli when a very rapid rate of stimulus delivery is used. On the basis of the subsequent set of experiments, the conditions and limits of the existence of the auditory 'N1 effect' are now quite clear. Moreover, this finding has been extended to somatosensory and visual modalities. In the present review a detailed examination of these studies has suggested a re-interpretation of the N1 effect. According to this reinterpretation, it is not a 'true' N1 component which is enhanced but the effect is produced by a summation of a negative shift with the evoked-potential wave form. Under some conditions such as those involving a very fast rate of stimulus delivery, this effect commences very early, making the N1 component appear larger. It is suggested that this shift reflects orienting to, and further processing of, an input found relevant in a preliminary sensory analysis. Topographical evidence for this kind of interpretation is provided by several studies. This negative shift is, hence, associated with voluntary attention. Some of the reviewed studies have described a rather similar negative shift as a correlate of involuntary attention to rare stimuli among the much more frequent, 'standard', stimuli."}
{"id": "PMID:465624", "title": "In vitro dissolution of Synthos ceramics in an acellular physiological environment.", "content": "\"Synthos\" (beta-tricalcium phosphate) implants in bone are resorbed and replaced with endogenous bone. This investigation was conducted to study by continuous flow and static system techniques, whether or not resorption of synthos occurs by passive dissolution at 37 degrees C. Calcium and phosphates were released in a time dependent manner from synthos in calcium and phosphate free Tris-Hcl buffer (pH 7.4). Scanning electron micrographs (SEM) of buffer exposed ceramics indicated breakdown of grain structure. In contrast, levels of human plasma calcium and phosphate were not altered by ceramics. Significant amounts of 32p were absorbed by the ceramics in the first hour and 45Ca in the second hour of exposure to plasma containing the radioactive isotopes. Exposure of ceramics to plasma did not alter the levels of plasm isotopes significantly for the remaining duration of the experiment. However, breakdown of grain structure was evident in SEM's of ceramics exposed to plasma. It appears that in an acellular physiological environment, dissolution of synthos is diffusion dependent and is limited to a localized surface exchange phenonmenon.", "contents": "In vitro dissolution of Synthos ceramics in an acellular physiological environment. \"Synthos\" (beta-tricalcium phosphate) implants in bone are resorbed and replaced with endogenous bone. This investigation was conducted to study by continuous flow and static system techniques, whether or not resorption of synthos occurs by passive dissolution at 37 degrees C. Calcium and phosphates were released in a time dependent manner from synthos in calcium and phosphate free Tris-Hcl buffer (pH 7.4). Scanning electron micrographs (SEM) of buffer exposed ceramics indicated breakdown of grain structure. In contrast, levels of human plasma calcium and phosphate were not altered by ceramics. Significant amounts of 32p were absorbed by the ceramics in the first hour and 45Ca in the second hour of exposure to plasma containing the radioactive isotopes. Exposure of ceramics to plasma did not alter the levels of plasm isotopes significantly for the remaining duration of the experiment. However, breakdown of grain structure was evident in SEM's of ceramics exposed to plasma. It appears that in an acellular physiological environment, dissolution of synthos is diffusion dependent and is limited to a localized surface exchange phenonmenon."}
{"id": "PMID:465626", "title": "Importance of design, control, & quantitative data in dental clinical research.", "content": "The ultimate test of a restorative dental biomaterial is its performance in vivo. Often laboratory test data does not correlate directly with clinical performance, so clinical research with human patients is essential. Clinical research that obtains maximum information from a minimum number of patients must be well designed and controlled. Pilot studies are necessary to establish experimental conditions and to determine the sample size. Previous clinical studies with various restorative materials have shown that careful screening and comprehensive oral examinations of patients are necessary to insure maximum recall for evaluation. Controlled placement procedures and randomized alloy placement are extremely important. Restorations are evaluated both by the response of patients to the restorations and by trained and calibrated dental examiners using established ranking criteria. Quantitative data measured in vivo such as that obtained from stereophotogrammetry reduced the need for large patient populations. The interfacing of a computer to record patient, placement, and evaluation data assisted greatly in project management.", "contents": "Importance of design, control, & quantitative data in dental clinical research. The ultimate test of a restorative dental biomaterial is its performance in vivo. Often laboratory test data does not correlate directly with clinical performance, so clinical research with human patients is essential. Clinical research that obtains maximum information from a minimum number of patients must be well designed and controlled. Pilot studies are necessary to establish experimental conditions and to determine the sample size. Previous clinical studies with various restorative materials have shown that careful screening and comprehensive oral examinations of patients are necessary to insure maximum recall for evaluation. Controlled placement procedures and randomized alloy placement are extremely important. Restorations are evaluated both by the response of patients to the restorations and by trained and calibrated dental examiners using established ranking criteria. Quantitative data measured in vivo such as that obtained from stereophotogrammetry reduced the need for large patient populations. The interfacing of a computer to record patient, placement, and evaluation data assisted greatly in project management."}
{"id": "PMID:465627", "title": "Implant failures in orthopaedic surgery.", "content": "Common orthopaedic implant failures are reviewed in the areas of total joint replacement and fracture fixation. In particular total hip and total knee arthroplasty, intertrochanteric hip fractures and long bone fractures are discussed. Excessive motion of implant bone interfaces, stress concentrations within the implant and stress shielding of bone are implicated in implant failures.", "contents": "Implant failures in orthopaedic surgery. Common orthopaedic implant failures are reviewed in the areas of total joint replacement and fracture fixation. In particular total hip and total knee arthroplasty, intertrochanteric hip fractures and long bone fractures are discussed. Excessive motion of implant bone interfaces, stress concentrations within the implant and stress shielding of bone are implicated in implant failures."}
{"id": "PMID:465628", "title": "Evaluation of the tissue response to the wear products of the hip joint endo-arthroprosthesis.", "content": "The causes of tissue reaction surrounding heterogeneous materials are: cell-mediated hypersensitivity due to an implant material; infection; post-surgery hematoma, following lysis of red cells and deposition of hemosiderin; \"True\" implant reaction, i.e., tissue modifications produced by wear particles of components of the prosthetic devices used. The stages of inflammation due to wear particles are: phagocytosis of particles by newly formed fibrous capsule cells; lymphatic drainage of particles through the newly formed joint capsule; granulation tissue reaction in the internal surface of the joint capsule which stores non-removed particles from inadequate drainage; necrosis of granulation tissue in contacting the artificial joint surface; formation of free masses of necrotic tissue in the joint cavity; formation of new granulation tissue due to these necrotic masses and by wear particles liberated from them (the beginning of a vicious cycle); insufficient storage by granulation tissue; formation of \"auxiliary\" granulation tissue in the fibrous layer of the bone-cement interfaces and in the R.E.S. of the bone-marrow; bone resorption and its replacement by granulation tissue; loosening of the prosthesis.", "contents": "Evaluation of the tissue response to the wear products of the hip joint endo-arthroprosthesis. The causes of tissue reaction surrounding heterogeneous materials are: cell-mediated hypersensitivity due to an implant material; infection; post-surgery hematoma, following lysis of red cells and deposition of hemosiderin; \"True\" implant reaction, i.e., tissue modifications produced by wear particles of components of the prosthetic devices used. The stages of inflammation due to wear particles are: phagocytosis of particles by newly formed fibrous capsule cells; lymphatic drainage of particles through the newly formed joint capsule; granulation tissue reaction in the internal surface of the joint capsule which stores non-removed particles from inadequate drainage; necrosis of granulation tissue in contacting the artificial joint surface; formation of free masses of necrotic tissue in the joint cavity; formation of new granulation tissue due to these necrotic masses and by wear particles liberated from them (the beginning of a vicious cycle); insufficient storage by granulation tissue; formation of \"auxiliary\" granulation tissue in the fibrous layer of the bone-cement interfaces and in the R.E.S. of the bone-marrow; bone resorption and its replacement by granulation tissue; loosening of the prosthesis."}
{"id": "PMID:465629", "title": "Developments in carbon prosthetics.", "content": "The majority of carbon-coated prosthetic devices in use today are coated with a unique form of carbon, low-temperature isotropic (LTI) carbon. The wide acceptance of this special form of carbon is a direct result of LTI carbon's demonstrated biocompatibility, its mechanical properties, and its inertness. The LTI carbon deposition process, however, places severe constraints on the size and type of substrate that can be coated. The substrates must be small so that they may be supported in a fluidized bed and further must be able to withstand temperatures in excess of 1200 degrees C. Recent technological advancements have removed the requirement that an object to be coated must be suspended in a fluidized bed and have also made possible the deposition of isotropic carbon at near room temperature. These developments expand the application of carbon-surfaced components into areas of prosthetics not previously possible. This paper describes some of the new applications and results.", "contents": "Developments in carbon prosthetics. The majority of carbon-coated prosthetic devices in use today are coated with a unique form of carbon, low-temperature isotropic (LTI) carbon. The wide acceptance of this special form of carbon is a direct result of LTI carbon's demonstrated biocompatibility, its mechanical properties, and its inertness. The LTI carbon deposition process, however, places severe constraints on the size and type of substrate that can be coated. The substrates must be small so that they may be supported in a fluidized bed and further must be able to withstand temperatures in excess of 1200 degrees C. Recent technological advancements have removed the requirement that an object to be coated must be suspended in a fluidized bed and have also made possible the deposition of isotropic carbon at near room temperature. These developments expand the application of carbon-surfaced components into areas of prosthetics not previously possible. This paper describes some of the new applications and results."}
{"id": "PMID:465630", "title": "Development of a shock absorbing biomedical elastomer for a new total elbow replacement design.", "content": "A shock absorbing concept of a total elbow replacement is presented. The development of a polyurethane elastomer with suitable damping and vibration characteristics, and the impact tester for comparative evaluation of elastic properties are described.", "contents": "Development of a shock absorbing biomedical elastomer for a new total elbow replacement design. A shock absorbing concept of a total elbow replacement is presented. The development of a polyurethane elastomer with suitable damping and vibration characteristics, and the impact tester for comparative evaluation of elastic properties are described."}
{"id": "PMID:465631", "title": "Biocompatibility of apatite ceramics in mandibles.", "content": "Apatite ceramics composed of synthetic hydroxyapatite, Ca10(PO4)6(OH)2 were implanted in mandibles of adults dogs. The histological observations indicated that the apatite ceramic was closely contacted with newly formed bone tissue without any rejection phenomenon up to 2 weeks. An electron micrograph of non-decalcified ultra-thin section at 8 weeks showed that the apatite ceramic was directly bonded to newly formed bone and the mineralized bone tissue was grown into the micro pores of the ceramics independent on the pore size. It seemed that the outer surface of the apatite was exchanged by bone tissue. From these results it was considered that the apatite ceramic was a successful implant material in dental and surgical fields.", "contents": "Biocompatibility of apatite ceramics in mandibles. Apatite ceramics composed of synthetic hydroxyapatite, Ca10(PO4)6(OH)2 were implanted in mandibles of adults dogs. The histological observations indicated that the apatite ceramic was closely contacted with newly formed bone tissue without any rejection phenomenon up to 2 weeks. An electron micrograph of non-decalcified ultra-thin section at 8 weeks showed that the apatite ceramic was directly bonded to newly formed bone and the mineralized bone tissue was grown into the micro pores of the ceramics independent on the pore size. It seemed that the outer surface of the apatite was exchanged by bone tissue. From these results it was considered that the apatite ceramic was a successful implant material in dental and surgical fields."}
{"id": "PMID:465632", "title": "Poly-HEMA sponge: a biocompatible calcification implant.", "content": "Poly-hydroxyethyl methacrylate (Poly-HEMA) was investigated for biocompatibility and calcification potential. S.C. and I.M. implants and transplants to subperiosteal sites were examined. The material was judged to be tolerated by the host tissue since no inflammatory or degenerative changes were observed. This study established that calcification does occur. This calcification does not simulate bone formation; osteoblasts were not observed. The x-ray diffraction pattern resembles that of calcium hydroxyapatite.", "contents": "Poly-HEMA sponge: a biocompatible calcification implant. Poly-hydroxyethyl methacrylate (Poly-HEMA) was investigated for biocompatibility and calcification potential. S.C. and I.M. implants and transplants to subperiosteal sites were examined. The material was judged to be tolerated by the host tissue since no inflammatory or degenerative changes were observed. This study established that calcification does occur. This calcification does not simulate bone formation; osteoblasts were not observed. The x-ray diffraction pattern resembles that of calcium hydroxyapatite."}
{"id": "PMID:465636", "title": "Family studies of red-cell metabolism of pyridoxine in beta-thalassaemia.", "content": "Red-cell conversion of pyridoxine to pyridoxal phosphate was measured in 12 families with thalassaemia and 2 normal families. A strong familial pattern was demonstrated and the evidence suggested that the rate of red-cell conversion of pyridoxine is an independently inherited characteristic.", "contents": "Family studies of red-cell metabolism of pyridoxine in beta-thalassaemia. Red-cell conversion of pyridoxine to pyridoxal phosphate was measured in 12 families with thalassaemia and 2 normal families. A strong familial pattern was demonstrated and the evidence suggested that the rate of red-cell conversion of pyridoxine is an independently inherited characteristic."}
{"id": "PMID:465637", "title": "Neutralization of immune complex inhibition of antibody dependent cellular cytotoxicity in vitro by Staphylococcus-aureus protein A.", "content": "Staphylococcus-aureus protein A (SPA) incubated with sera containing immune complexes and effector cells increases antibody dependent cellular cytotoxicity (ADCC)and rosette formation. The enhanced ADCC and rosetting is due to SPA functioning as an IgG Fc cellular receptor. SPA binds to the Fc portion of Igg and has much greater affinity for immune complexes than free IgG. SPA bound immune complexes are blocked from attaching to the Fc cellular receptors via a competitive inhibition which neutralizes the inhibitory effect of these complexes on ADCC and rosette formation.", "contents": "Neutralization of immune complex inhibition of antibody dependent cellular cytotoxicity in vitro by Staphylococcus-aureus protein A. Staphylococcus-aureus protein A (SPA) incubated with sera containing immune complexes and effector cells increases antibody dependent cellular cytotoxicity (ADCC)and rosette formation. The enhanced ADCC and rosetting is due to SPA functioning as an IgG Fc cellular receptor. SPA binds to the Fc portion of Igg and has much greater affinity for immune complexes than free IgG. SPA bound immune complexes are blocked from attaching to the Fc cellular receptors via a competitive inhibition which neutralizes the inhibitory effect of these complexes on ADCC and rosette formation."}
{"id": "PMID:465638", "title": "Surface and cytoplasmic immunoglobulin distribution during treatment of a non-excretory myeloma.", "content": "A patient with non-excretory myeloma with severe clinical signs, has been assessed for cytoplasmic and surface immunoglobulins on peripheral mononuclear cells and bone marrow cells. A clearcut monoclonal pattern was found. During treatment with melphalan, methotrexate and prednisolone a clinical remission was obtained. The immunological studies were repeated during this remission and were in accordance with the clinical improvement, however the monoclonality persisted and indicated an intracellular dissocitaion of light and heavy chains. The patient died with broncho-pneumonia and marrow failure.", "contents": "Surface and cytoplasmic immunoglobulin distribution during treatment of a non-excretory myeloma. A patient with non-excretory myeloma with severe clinical signs, has been assessed for cytoplasmic and surface immunoglobulins on peripheral mononuclear cells and bone marrow cells. A clearcut monoclonal pattern was found. During treatment with melphalan, methotrexate and prednisolone a clinical remission was obtained. The immunological studies were repeated during this remission and were in accordance with the clinical improvement, however the monoclonality persisted and indicated an intracellular dissocitaion of light and heavy chains. The patient died with broncho-pneumonia and marrow failure."}
{"id": "PMID:465640", "title": "A study on sleep in amyotrophic lateral sclerosis.", "content": "A polygraphic study of sleep has been performed on 12 patients with ALS and 12 normal controls. Neither the total duration of sleep, nor the proportion of different stages of sleep differed significantly between the patient and control groups. A variety of morphologic peculiarities were observed in certain patients: frequent and repetitive blinking movements during the waking state (under quiet, dark conditions); persistence of EMG activity during both PS and SWS; and an early appearance of marked slow delta wave activity at the beginning of sleep. We observed in patients increased PS latency, and longer and more frequent awakenings. Certain patients also showed both qualitative and quantitative abnormalities of respiratory rhythm. A longitudinal study was performed on one patient. Results are discussed in relation to the existing literature.", "contents": "A study on sleep in amyotrophic lateral sclerosis. A polygraphic study of sleep has been performed on 12 patients with ALS and 12 normal controls. Neither the total duration of sleep, nor the proportion of different stages of sleep differed significantly between the patient and control groups. A variety of morphologic peculiarities were observed in certain patients: frequent and repetitive blinking movements during the waking state (under quiet, dark conditions); persistence of EMG activity during both PS and SWS; and an early appearance of marked slow delta wave activity at the beginning of sleep. We observed in patients increased PS latency, and longer and more frequent awakenings. Certain patients also showed both qualitative and quantitative abnormalities of respiratory rhythm. A longitudinal study was performed on one patient. Results are discussed in relation to the existing literature."}
{"id": "PMID:465641", "title": "The effect of high glucose concentration on the proliferation and the synthesis of lipids in endothelial cells in culture.", "content": "Influence of glucose concentration on growth and lipid synthesis was investigated in human endothelial cells in culture. The biosynthesis of lipids from acetate and glucose was present in endothelial cells, but a high glucose level in the culture medium failed to modify lipid synthesis. Neosynthesis in the lipidic fraction in the medium is higher when precursor is acetate compared to glucose.", "contents": "The effect of high glucose concentration on the proliferation and the synthesis of lipids in endothelial cells in culture. Influence of glucose concentration on growth and lipid synthesis was investigated in human endothelial cells in culture. The biosynthesis of lipids from acetate and glucose was present in endothelial cells, but a high glucose level in the culture medium failed to modify lipid synthesis. Neosynthesis in the lipidic fraction in the medium is higher when precursor is acetate compared to glucose."}
{"id": "PMID:465642", "title": "Interaction of nucleic acids with electrically charged surfaces. VI. A comparative study on the electrochemical behaviour of native and denatured DNAs at graphite electrodes.", "content": "Adsorption and electrochemical oxidation of deoxyribonucleic acid (DNA) at a pyrolytic graphite electrode (PGE) and a paraffin wax-impregnated spectroscopic graphite electrode (WISGE) were studied using differential pulse voltammetry. DNA is adsorbed at the surface of the graphite electrodes in a broad range of potentials including the potentials of electrochemical oxidation of DNA. Both native and denatured DNAs yield two single, well-defined and separated peaks, G and A, on the differential pulse voltammograms at the PGE and WISGE. The more negative peak, G, corresponds to electrochemical oxidation of adenine residues. Peaks G and A of native DNA occur at the same potentials as peaks G and A of denatured DNA. However, electrochemical oxidation of adenine and guanine residues at graphite electrodes is markedly suppressed in native DNA. The heights of the peaks G and A represent a sensitive indicator of the helix-coil transition of DNA. An analysis of the product of interaction of a sample of native DNA with a large pyrolytic graphite electrode in the presence of formaldehyde at approximately neutral pH did not prove changes in the secondary structure of native DNA due to its interaction with the graphite electrode. It is suggested that the decreased differential pulse-voltammetric activity of native DNA is connected with its decreased flexibility.", "contents": "Interaction of nucleic acids with electrically charged surfaces. VI. A comparative study on the electrochemical behaviour of native and denatured DNAs at graphite electrodes. Adsorption and electrochemical oxidation of deoxyribonucleic acid (DNA) at a pyrolytic graphite electrode (PGE) and a paraffin wax-impregnated spectroscopic graphite electrode (WISGE) were studied using differential pulse voltammetry. DNA is adsorbed at the surface of the graphite electrodes in a broad range of potentials including the potentials of electrochemical oxidation of DNA. Both native and denatured DNAs yield two single, well-defined and separated peaks, G and A, on the differential pulse voltammograms at the PGE and WISGE. The more negative peak, G, corresponds to electrochemical oxidation of adenine residues. Peaks G and A of native DNA occur at the same potentials as peaks G and A of denatured DNA. However, electrochemical oxidation of adenine and guanine residues at graphite electrodes is markedly suppressed in native DNA. The heights of the peaks G and A represent a sensitive indicator of the helix-coil transition of DNA. An analysis of the product of interaction of a sample of native DNA with a large pyrolytic graphite electrode in the presence of formaldehyde at approximately neutral pH did not prove changes in the secondary structure of native DNA due to its interaction with the graphite electrode. It is suggested that the decreased differential pulse-voltammetric activity of native DNA is connected with its decreased flexibility."}
{"id": "PMID:465643", "title": "Scanning molecular sieve chromatography of interacting protein systems. II. Determination of large zone transport parameters by the difference profile method at low solute concentration.", "content": "The experimental determination of difference profiles for the study of large zone transport processes by scanning molecular sieve chromatography is described. Using the difference profile method, the progesterone-induced purple glycoprotein of the porcine uterus was found to exist as monomeric units in high ionic environment, with a partition coefficient of 0.269, partition cross-section of 0.488, partition radius of 25 A and a molecular weight of 33,500 g/mole. The technique was further applied in examining the association-dissociation properties of oxyhemoglobin. In a high tonic environment, the partition coefficient was found to be 0.365 for dimer and the partition cross-section, 0.419; for the tetramer in low ionic strength solution, the partition coefficient was 0.275 and the partition cross-section 0.377, with a dissociation constant of 1.03 x 10(-6) mole/l. This new technique should prove applicable in (1) readily locating the centroid positions of transport boundary profiles at the lowest practible protein concentration limits, (2) demonstrating the characteristic boundary shape and concentration-dependent centroid position for an interacting solute, (3) determining the axial dispersion coefficient characteristic of solute turbulence within the gel matrix, and (4) distinguishing the boundary between low and high ionic strength solvent phases in the gel column.", "contents": "Scanning molecular sieve chromatography of interacting protein systems. II. Determination of large zone transport parameters by the difference profile method at low solute concentration. The experimental determination of difference profiles for the study of large zone transport processes by scanning molecular sieve chromatography is described. Using the difference profile method, the progesterone-induced purple glycoprotein of the porcine uterus was found to exist as monomeric units in high ionic environment, with a partition coefficient of 0.269, partition cross-section of 0.488, partition radius of 25 A and a molecular weight of 33,500 g/mole. The technique was further applied in examining the association-dissociation properties of oxyhemoglobin. In a high tonic environment, the partition coefficient was found to be 0.365 for dimer and the partition cross-section, 0.419; for the tetramer in low ionic strength solution, the partition coefficient was 0.275 and the partition cross-section 0.377, with a dissociation constant of 1.03 x 10(-6) mole/l. This new technique should prove applicable in (1) readily locating the centroid positions of transport boundary profiles at the lowest practible protein concentration limits, (2) demonstrating the characteristic boundary shape and concentration-dependent centroid position for an interacting solute, (3) determining the axial dispersion coefficient characteristic of solute turbulence within the gel matrix, and (4) distinguishing the boundary between low and high ionic strength solvent phases in the gel column."}
{"id": "PMID:465644", "title": "Scanning molecular sieve chromatography of interacting protein systems. III. Effect of kinetic parameters on the large zone boundary profiles for local equilibration between mobile and stationary phases.", "content": "Large zone reaction boundary profiles for molecular sieve chromatography as affected by kinetic parameters have been simulated for local equilibration between the mobile and stationary phases. Our studies of monomer-dimer and monomer-tetramer systems indicate that in a slowly equilibrating system, the kinetic controls operating between the mobile and stationary phases contribute most significantly to the overall boundary profile. In a rapidly equilibrating system, however, the kinetic parameters kij and kji operating in the mobile phase are the principal determinants of the reaction boundary, while the kinetic effects of kii and k-ii between the mobile and stationary phases are minimal.", "contents": "Scanning molecular sieve chromatography of interacting protein systems. III. Effect of kinetic parameters on the large zone boundary profiles for local equilibration between mobile and stationary phases. Large zone reaction boundary profiles for molecular sieve chromatography as affected by kinetic parameters have been simulated for local equilibration between the mobile and stationary phases. Our studies of monomer-dimer and monomer-tetramer systems indicate that in a slowly equilibrating system, the kinetic controls operating between the mobile and stationary phases contribute most significantly to the overall boundary profile. In a rapidly equilibrating system, however, the kinetic parameters kij and kji operating in the mobile phase are the principal determinants of the reaction boundary, while the kinetic effects of kii and k-ii between the mobile and stationary phases are minimal."}
{"id": "PMID:465645", "title": "Scanning molecular sieve chromatography of interacting protein systems. IV. The difference profile method as applied to the Gibbs-Duhem expression in the analysis of the dimer-tetramer equilibria of oxyhemoglobin A.", "content": "The recently-developed large zone difference profile method in scanning molecular sieve chromatography is applied to the analysis of the Gibbs-Duhem expression in the tetramer-dimer equilibrium of human oxyhemoglobin A. The preferential binding term and solvation parameters of the Hofmeister anion phosphate are examined. Results indicate that as the concentration of phosphate ions increase, a hydrated phosphate is formed which enhances the association by perturbing the solvation layer of the hemoglobin molecules. The standard free energy change at a given Hofmeister anion activity of ln Ax = -3.2476 is 9.4 +/- 0.2 kcal/mole. delta G0 at ln Ax = -1.2711 is 10.90 +/- 0.05 kcal/mole, suggesting that approximately 11 kcal are required to dissociate one mole of tetramer into dimer.", "contents": "Scanning molecular sieve chromatography of interacting protein systems. IV. The difference profile method as applied to the Gibbs-Duhem expression in the analysis of the dimer-tetramer equilibria of oxyhemoglobin A. The recently-developed large zone difference profile method in scanning molecular sieve chromatography is applied to the analysis of the Gibbs-Duhem expression in the tetramer-dimer equilibrium of human oxyhemoglobin A. The preferential binding term and solvation parameters of the Hofmeister anion phosphate are examined. Results indicate that as the concentration of phosphate ions increase, a hydrated phosphate is formed which enhances the association by perturbing the solvation layer of the hemoglobin molecules. The standard free energy change at a given Hofmeister anion activity of ln Ax = -3.2476 is 9.4 +/- 0.2 kcal/mole. delta G0 at ln Ax = -1.2711 is 10.90 +/- 0.05 kcal/mole, suggesting that approximately 11 kcal are required to dissociate one mole of tetramer into dimer."}
{"id": "PMID:465646", "title": "Multisite proteins and randomly coiled polymeric ligands. Chain length dependence of binding constants.", "content": "A model mechanism was developed for the binding of a rigid multisite protein with a randomly coiled multivalent ligand. Probabilities of the formation of chain loops between sites located at given distances at the protein were calculated by an extension of the concept of ring closure in coiled chain molecules. Expressions were derived for the dependence of overall equilibrium quantities, such as the binding constant between the protein and the ligand, on intrinsic parameters such as intrinsic binding constants, number of sites at the protein and their distances and on the chain length of the polymeric ligand. A pronounced chain length dependence of the overall binding constant was predicted even at chain lengths much longer than the size of the protein. Such a dependence was previously observed for the enzyme prolyl hydroxylase which acts on polymeric substrates like (ProProGly)n. This so far unexplained feature is quantitatively described by the model mechanism which is believed to be applicable to many other interactions of biological importance.", "contents": "Multisite proteins and randomly coiled polymeric ligands. Chain length dependence of binding constants. A model mechanism was developed for the binding of a rigid multisite protein with a randomly coiled multivalent ligand. Probabilities of the formation of chain loops between sites located at given distances at the protein were calculated by an extension of the concept of ring closure in coiled chain molecules. Expressions were derived for the dependence of overall equilibrium quantities, such as the binding constant between the protein and the ligand, on intrinsic parameters such as intrinsic binding constants, number of sites at the protein and their distances and on the chain length of the polymeric ligand. A pronounced chain length dependence of the overall binding constant was predicted even at chain lengths much longer than the size of the protein. Such a dependence was previously observed for the enzyme prolyl hydroxylase which acts on polymeric substrates like (ProProGly)n. This so far unexplained feature is quantitatively described by the model mechanism which is believed to be applicable to many other interactions of biological importance."}
{"id": "PMID:465678", "title": "[Correlation of atrial and ventricular contractility. The possibility of using the atria for controlling the artificial heart].", "content": "A possibility of the artificial ventricle regulation using information on the atrial contraction is discussed. Correlations between atrial wall tension and ventricle tension as well as the pressure under radom heart rhythm variations were studied. The wall tension of the appropriate heart portion was recorded by the arched tensiometers, and intraventricular pressure under cavity catheterization by the electric manometer. The cardiac rhythm varied between 2.0 c-1 and 4.0 c-1. A correlation between the tension and interpulse interval, (correlation coefficient being 0.62 +/- 0.05) was established. The close relationships \"atrial wall tension-intramuscular pressure\" were observed. For such relationships the correlation coefficient changed from 0.713 +/- 0,09 to 0,874 +/- 0.02 depending on the average value of interpulse interval. It is concluded that the information on the atrial contraction can be used for artificial heart regulation.", "contents": "[Correlation of atrial and ventricular contractility. The possibility of using the atria for controlling the artificial heart]. A possibility of the artificial ventricle regulation using information on the atrial contraction is discussed. Correlations between atrial wall tension and ventricle tension as well as the pressure under radom heart rhythm variations were studied. The wall tension of the appropriate heart portion was recorded by the arched tensiometers, and intraventricular pressure under cavity catheterization by the electric manometer. The cardiac rhythm varied between 2.0 c-1 and 4.0 c-1. A correlation between the tension and interpulse interval, (correlation coefficient being 0.62 +/- 0.05) was established. The close relationships \"atrial wall tension-intramuscular pressure\" were observed. For such relationships the correlation coefficient changed from 0.713 +/- 0,09 to 0,874 +/- 0.02 depending on the average value of interpulse interval. It is concluded that the information on the atrial contraction can be used for artificial heart regulation."}
{"id": "PMID:465679", "title": "[Hyperventilation and inhibitory synapses].", "content": "Injection of subconvulsive doses of strychnine blocking the inhibitory synapses significantly increases the reflex activity of the respiratory muscle evoked by stimulation of the sciatic nerve as well as by inhalation of hypercapnic gas mixture. Thus the inhibitory synapses prevent the extreme hypocapnia evoked by hyperventilation.", "contents": "[Hyperventilation and inhibitory synapses]. Injection of subconvulsive doses of strychnine blocking the inhibitory synapses significantly increases the reflex activity of the respiratory muscle evoked by stimulation of the sciatic nerve as well as by inhalation of hypercapnic gas mixture. Thus the inhibitory synapses prevent the extreme hypocapnia evoked by hyperventilation."}
{"id": "PMID:465680", "title": "[Relationship of the activity of cardiac cholinergic mechanisms to the state of the sympathetic innervation and its noradrenaline content in hypoxia].", "content": "Preliminary sympathectomia depletes acetylcholine (ACh) in the heart of rabbits under hypoxia. In these conditions the inhibitory action of ACh on the rat isolated heart is reduced under the noradrenaline content fall, while under increase it is potentiated. Under hypoxia noradrenaline increases concentration of potassium in the myocardium, thus stimulating ACh formation and activity. It is suggested that under deep hypoxia suppression of the sympathetic mechanisms causes functional isolation of the heart from nervous effects.", "contents": "[Relationship of the activity of cardiac cholinergic mechanisms to the state of the sympathetic innervation and its noradrenaline content in hypoxia]. Preliminary sympathectomia depletes acetylcholine (ACh) in the heart of rabbits under hypoxia. In these conditions the inhibitory action of ACh on the rat isolated heart is reduced under the noradrenaline content fall, while under increase it is potentiated. Under hypoxia noradrenaline increases concentration of potassium in the myocardium, thus stimulating ACh formation and activity. It is suggested that under deep hypoxia suppression of the sympathetic mechanisms causes functional isolation of the heart from nervous effects."}
{"id": "PMID:465681", "title": "[Hemoglobin, transferrin and total iron content in the blood serum in hyperoxia and during the protective action of urea].", "content": "A rise of hemoglobin concentration accompanied by an increase of the total iron in the blood serum of white mice was found under oxygen pressure of 4 atm for an hour (preconvulsive state) and 6 atm (convulsive state). Changes in correlations of hemoglobin fractions in the blood serum were detected in both stages of oxygen poisoning by disc-electrophoresis in 7.5% polyacrylamide gel. A rise of transferrin concentration under these conditions (hyperoxia) was observed. The deflections occurred were less pronounced following administration of urea to the animals before hyperbaric oxygenation.", "contents": "[Hemoglobin, transferrin and total iron content in the blood serum in hyperoxia and during the protective action of urea]. A rise of hemoglobin concentration accompanied by an increase of the total iron in the blood serum of white mice was found under oxygen pressure of 4 atm for an hour (preconvulsive state) and 6 atm (convulsive state). Changes in correlations of hemoglobin fractions in the blood serum were detected in both stages of oxygen poisoning by disc-electrophoresis in 7.5% polyacrylamide gel. A rise of transferrin concentration under these conditions (hyperoxia) was observed. The deflections occurred were less pronounced following administration of urea to the animals before hyperbaric oxygenation."}
{"id": "PMID:465682", "title": "[Hematopoietic study of rats in experiments with exchange blood replacement by an emulsion of perfluorotributylamine].", "content": "In experiments on nonanesthetized rats blood was substituted with perfluorotributyl emulsion (PFTBA). The mean volume of transfused PFTBA was 10 ml/100 g of body weight. During the period of blood replacement and the following 5 days the rats were kept in an atmosphere with increased oxygen content, and then they were placed under normal atmospheric conditions. The influence of PFTBA solution on hemopoiesis was studied in these experiments. A marked erythropoiesis activation in the bone marrow combined with expressed peripheral blood reticulocytosis was observed 6 days after the exchange blood substitution. Hemoglobin content decreased to 8.2 g% mature and immature white cell count rose; immature erythropoiesis cells were found: polychromatophilic erythro- and normoblasts (4.2% on the average). By the 13th day there was complete normalization of hemopoiesis which lasted 12 months without any significant deviations. Basing upon the results obtained it may be concluded that the PFTBA emulsion under study produces no negative effect on the animal hemopoiesis, and does not disturb the normal regulation process under conditions of the experiment conducted.", "contents": "[Hematopoietic study of rats in experiments with exchange blood replacement by an emulsion of perfluorotributylamine]. In experiments on nonanesthetized rats blood was substituted with perfluorotributyl emulsion (PFTBA). The mean volume of transfused PFTBA was 10 ml/100 g of body weight. During the period of blood replacement and the following 5 days the rats were kept in an atmosphere with increased oxygen content, and then they were placed under normal atmospheric conditions. The influence of PFTBA solution on hemopoiesis was studied in these experiments. A marked erythropoiesis activation in the bone marrow combined with expressed peripheral blood reticulocytosis was observed 6 days after the exchange blood substitution. Hemoglobin content decreased to 8.2 g% mature and immature white cell count rose; immature erythropoiesis cells were found: polychromatophilic erythro- and normoblasts (4.2% on the average). By the 13th day there was complete normalization of hemopoiesis which lasted 12 months without any significant deviations. Basing upon the results obtained it may be concluded that the PFTBA emulsion under study produces no negative effect on the animal hemopoiesis, and does not disturb the normal regulation process under conditions of the experiment conducted."}
{"id": "PMID:465683", "title": "[Effect of the duration of the previous ischemia and of the mass of ischemic tissue on the state of the coagulating and anticoagulating blood systems in tourniquet shock].", "content": "In the experiments on 80 rabbits it was shown that the inclusion of the previously ischemized extremities into circulation is accompanied by elevation of coagulating potential of the blood and inhibition of fibrinolysis shortly after the tourniquet removal followed by hypocoagulation and fibrinolysis activation. These changes depend on the duration and mass of previously ischemized tissues. It is suggested that in tourniquet shock these disorders lead either to the danger of intravascular thrombosis or to hypocoagulation with secondary fibrinolysis.", "contents": "[Effect of the duration of the previous ischemia and of the mass of ischemic tissue on the state of the coagulating and anticoagulating blood systems in tourniquet shock]. In the experiments on 80 rabbits it was shown that the inclusion of the previously ischemized extremities into circulation is accompanied by elevation of coagulating potential of the blood and inhibition of fibrinolysis shortly after the tourniquet removal followed by hypocoagulation and fibrinolysis activation. These changes depend on the duration and mass of previously ischemized tissues. It is suggested that in tourniquet shock these disorders lead either to the danger of intravascular thrombosis or to hypocoagulation with secondary fibrinolysis."}
{"id": "PMID:465684", "title": "[Change in the physicochemical properties of the cerebral cortical proteins in dying and resuscitation after mechanical asphyxia].", "content": "The physicochemical properties of protein postmitochondrial supernatant after gray substance of the brain homogenization undergo changes during clinical death caused by mechanical asphyxia. The shift on the densitogram occurs after protein electrophoresis in polyacrilamide gel. After sedimentation the total concentration of proteins and total activity of lactate dehydrogenase (LDH) decrease. Followiing adsorption on a DEAE-Sephadex A-50 the total fraction of nonadsorbed beta- and gamma-globulins also decreases, and the activity of LDH (3+4+5) isoenzymes falls significantly. Disturbed behavior pattern of proteins in the sedimentation and electrophoretic fields as well as changes in the sorption properties specific for varying times of clinical death, play a certain role in the development of irreversible changes in the gray substance of the brain. The adsorption and sedimentation properties of the proteins return to normal during early postresuscitation period, whereas the electrophoretic mobility of protein fractions in polyacrylamide gel remains unchanged within the first day.", "contents": "[Change in the physicochemical properties of the cerebral cortical proteins in dying and resuscitation after mechanical asphyxia]. The physicochemical properties of protein postmitochondrial supernatant after gray substance of the brain homogenization undergo changes during clinical death caused by mechanical asphyxia. The shift on the densitogram occurs after protein electrophoresis in polyacrilamide gel. After sedimentation the total concentration of proteins and total activity of lactate dehydrogenase (LDH) decrease. Followiing adsorption on a DEAE-Sephadex A-50 the total fraction of nonadsorbed beta- and gamma-globulins also decreases, and the activity of LDH (3+4+5) isoenzymes falls significantly. Disturbed behavior pattern of proteins in the sedimentation and electrophoretic fields as well as changes in the sorption properties specific for varying times of clinical death, play a certain role in the development of irreversible changes in the gray substance of the brain. The adsorption and sedimentation properties of the proteins return to normal during early postresuscitation period, whereas the electrophoretic mobility of protein fractions in polyacrylamide gel remains unchanged within the first day."}
{"id": "PMID:465685", "title": "[Nonspecific esterase and alpha-glycerolphosphate dehydrogenase activity and the fat content in the regenerating chicken liver].", "content": "The data on histochemical study of changes in the activity of esterases, alpha-glycerophosphate dehydrogenase and the fat content in the chick regenerating liver are presented. The accumulation of the fat in hepatocytes during the early periods of regeneration (1--5th day of the experiment) is partially conditioned by the reduction of the activity of nonspecific esterases. The maximal liver steatosis is accompanied also by an increase in the activity of alpha-glycerophosphate dehydrogenase which indicates intensification of glycolysis and synthesis of triacylglycerines (liver spare lipids) during regeneration.", "contents": "[Nonspecific esterase and alpha-glycerolphosphate dehydrogenase activity and the fat content in the regenerating chicken liver]. The data on histochemical study of changes in the activity of esterases, alpha-glycerophosphate dehydrogenase and the fat content in the chick regenerating liver are presented. The accumulation of the fat in hepatocytes during the early periods of regeneration (1--5th day of the experiment) is partially conditioned by the reduction of the activity of nonspecific esterases. The maximal liver steatosis is accompanied also by an increase in the activity of alpha-glycerophosphate dehydrogenase which indicates intensification of glycolysis and synthesis of triacylglycerines (liver spare lipids) during regeneration."}
{"id": "PMID:465686", "title": "[Role of the GABA-ergic systems of the brain in manifesting the activating effect of diazepam].", "content": "It was shown in experiments on rats that the selective blocker of GABA receptors bicuculline (2 mg/kg) does not decrease the activating effect of diazepam as to the reaction of self-stimulation. The GABA-mimetic muscimol (0.5 and 1 mg/kg) had no effect on self-stimulation rate, while in the dose of 2 mg/kg causing behavioral changes produced a powerful decrease in it (by 93.3%). During the combined administration of diazepam and muscimol (1 mg/kg and 0.5 mg/kg, respectively) no potentiation of diazepam effect was observed. It is suggested that diazepam-induced facilitation of the reaction of self-stimulation is not due to the alteration in the activity of GABA-ergic processes.", "contents": "[Role of the GABA-ergic systems of the brain in manifesting the activating effect of diazepam]. It was shown in experiments on rats that the selective blocker of GABA receptors bicuculline (2 mg/kg) does not decrease the activating effect of diazepam as to the reaction of self-stimulation. The GABA-mimetic muscimol (0.5 and 1 mg/kg) had no effect on self-stimulation rate, while in the dose of 2 mg/kg causing behavioral changes produced a powerful decrease in it (by 93.3%). During the combined administration of diazepam and muscimol (1 mg/kg and 0.5 mg/kg, respectively) no potentiation of diazepam effect was observed. It is suggested that diazepam-induced facilitation of the reaction of self-stimulation is not due to the alteration in the activity of GABA-ergic processes."}
{"id": "PMID:465688", "title": "[Reactions of normal human and rabbit immunoglobulins with heart valve fibroblasts].", "content": "It was demonstrated using the indirect immunofluorescent technique that normal human and rabbit sera, and IgG isolated from them intensively reacted with fibroblasts of human and bovine heart valves. The results obtained with Fab and Fc fragments of IgG sugges that this reaction is due to the Fc region of the IgG molecule and related to the presence of the Fc receptor on fibroblasts of heart valves.", "contents": "[Reactions of normal human and rabbit immunoglobulins with heart valve fibroblasts]. It was demonstrated using the indirect immunofluorescent technique that normal human and rabbit sera, and IgG isolated from them intensively reacted with fibroblasts of human and bovine heart valves. The results obtained with Fab and Fc fragments of IgG sugges that this reaction is due to the Fc region of the IgG molecule and related to the presence of the Fc receptor on fibroblasts of heart valves."}
{"id": "PMID:465689", "title": "[Immunochemical study of the CBA mouse kidney].", "content": "Immunoelectrophoresis and agar precipitation reaction allowed one to reveal 7 antigens in CBA mouse kidney. One of them, with a relative electrophoretic mobility--0.35, is specific for the kidney. The remaining renal antigens are common to the kidney and other organs and tissues of mice. The antigens with mobility in the albumin and alpha-globulin zones proved to be serum ones. An antigen with a mobility approximating 0 is specific for the kidney and liver. An antigen with a mobility in the alpha 2-globulin zone does not seem likely to be homogeneous and, apart from broad inter-organ specificity, bears the greatest resemblance to the lungs antigen.", "contents": "[Immunochemical study of the CBA mouse kidney]. Immunoelectrophoresis and agar precipitation reaction allowed one to reveal 7 antigens in CBA mouse kidney. One of them, with a relative electrophoretic mobility--0.35, is specific for the kidney. The remaining renal antigens are common to the kidney and other organs and tissues of mice. The antigens with mobility in the albumin and alpha-globulin zones proved to be serum ones. An antigen with a mobility approximating 0 is specific for the kidney and liver. An antigen with a mobility in the alpha 2-globulin zone does not seem likely to be homogeneous and, apart from broad inter-organ specificity, bears the greatest resemblance to the lungs antigen."}
{"id": "PMID:465690", "title": "[Selective inhibition of the primary humoral immune response in mice with a combination of deoxycytidine with Cytosar].", "content": "Cytosine arabinoside administration in lethal doses to C57BL/6j female mice immunized with red blood cells leads, under deoxycytidine protection, to reduction of serum hemagglutinin level on day 5 without toxicosis. Simultaneous injection of the metabolite and the antimetabolite proves to be optimum.", "contents": "[Selective inhibition of the primary humoral immune response in mice with a combination of deoxycytidine with Cytosar]. Cytosine arabinoside administration in lethal doses to C57BL/6j female mice immunized with red blood cells leads, under deoxycytidine protection, to reduction of serum hemagglutinin level on day 5 without toxicosis. Simultaneous injection of the metabolite and the antimetabolite proves to be optimum."}
{"id": "PMID:465691", "title": "[Erythroid precursors studied by mouse bone marrow cultivation in a plasma clot].", "content": "Two types of erythroid precursors were found by cultivation of the mouse bone marrow in the plasma clot with mouse serum and without adding exogenic erythropoietin to the culture medium. The first precursor had properties similar to the erythroid colony-forming unit (CFUe) previously described while the second resembles in its properties the erythroid burst-forming unit (BFUe). Optimal concentration of mouse serum in the culture medium was 10-15%. Clone nature of the colonies and bursts described is confirmed by linear dependence of their number on the cell concentration in the culture.", "contents": "[Erythroid precursors studied by mouse bone marrow cultivation in a plasma clot]. Two types of erythroid precursors were found by cultivation of the mouse bone marrow in the plasma clot with mouse serum and without adding exogenic erythropoietin to the culture medium. The first precursor had properties similar to the erythroid colony-forming unit (CFUe) previously described while the second resembles in its properties the erythroid burst-forming unit (BFUe). Optimal concentration of mouse serum in the culture medium was 10-15%. Clone nature of the colonies and bursts described is confirmed by linear dependence of their number on the cell concentration in the culture."}
{"id": "PMID:465693", "title": "[Pathology of mitosis after cellular recovery from a metaphase block].", "content": "The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis.", "contents": "[Pathology of mitosis after cellular recovery from a metaphase block]. The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis."}
{"id": "PMID:465695", "title": "[Circardian rhythm of mitoses in the epithelium of the cornea after splenectomy].", "content": "In corneal epithelium of CBA mice the index of colchicine mitoses diminished after splenectomy in the day period characterized by rising mitotic activity in control animals. The duration of active phase of cell division rhythm shortened while the maximum of mitotic activity delayed in comparison with control animals. The total amount of cells entering mitosis during 24 hours diminished by 27.7% and the rate of physiological regeneration of corneal epithelium decreased.", "contents": "[Circardian rhythm of mitoses in the epithelium of the cornea after splenectomy]. In corneal epithelium of CBA mice the index of colchicine mitoses diminished after splenectomy in the day period characterized by rising mitotic activity in control animals. The duration of active phase of cell division rhythm shortened while the maximum of mitotic activity delayed in comparison with control animals. The total amount of cells entering mitosis during 24 hours diminished by 27.7% and the rate of physiological regeneration of corneal epithelium decreased."}
{"id": "PMID:465696", "title": "[Effect of the balanced inhibition of DNA and protein synthesis on the in vitro and in vivo proliferative activity of the lymphocytes].", "content": "Lymphocytes isolated from the rabbit peripheral blood were irradiated in vitro with 200, 400 and 1000 r doses and cultivated with phytohemagglutinin (PHA) doses and cultivated with phytohemagglutinin (PHA) at 37 degrees C during 48 hours. In several experiments cycloheximide, inhibiting protein synthesis, was added to the cells 60 minutes before and 30 minutes after irradiation. There was no apparent difference in the viability of irradiated cells with or without cycloheximide. The ability of lymphocytes of the popliteal lymph nodes for proliferation after PHA injection into one of the hind foot-pads of the irradiated mice was studied, as well. The injection of cycloheximide or puromycin into one of the hind foot-pads immediately after irradiation of the animals augmented the proliferation of lymphocytes in this extremity in comparison with contralateral one, 1.5-2 times. Cytosine arabinoside, inhibiting DNA synthesis, was not effective under these conditions.", "contents": "[Effect of the balanced inhibition of DNA and protein synthesis on the in vitro and in vivo proliferative activity of the lymphocytes]. Lymphocytes isolated from the rabbit peripheral blood were irradiated in vitro with 200, 400 and 1000 r doses and cultivated with phytohemagglutinin (PHA) doses and cultivated with phytohemagglutinin (PHA) at 37 degrees C during 48 hours. In several experiments cycloheximide, inhibiting protein synthesis, was added to the cells 60 minutes before and 30 minutes after irradiation. There was no apparent difference in the viability of irradiated cells with or without cycloheximide. The ability of lymphocytes of the popliteal lymph nodes for proliferation after PHA injection into one of the hind foot-pads of the irradiated mice was studied, as well. The injection of cycloheximide or puromycin into one of the hind foot-pads immediately after irradiation of the animals augmented the proliferation of lymphocytes in this extremity in comparison with contralateral one, 1.5-2 times. Cytosine arabinoside, inhibiting DNA synthesis, was not effective under these conditions."}
{"id": "PMID:465697", "title": "[Change in the dependence of lymphocyte reactivity to phytomitogens on endogenous hormonal factors in experimental bacterial lesion of the prostate].", "content": "The relationship between the blast lymphocyte transformation response (BLTR) to PHA P, Con A, PWM and 11-hydroxycorticosteroids (I) and the testosterone (II) levels in the blood plasma and the excretion of 17-ketosteroids with the urine were studied in 11 dogs with experimental urogenous prostatitis, provoked by Staphylococcus aureus, isolated from a patient with chronic prostatitis. Multiple correlation between the BLTR and hormonal factors was noted before the experiment. It was disturbed in a month and restored 2 months after the beginning of the experiment. Before the studies the relationships were mainly realized by direct correlation with 1 and 2 months after the experiment--due to the negative correlation with II. A homeostatic nature of the hormone lymphoid relationship with respect to 11-hydroxy-corticosteroids is suggested.", "contents": "[Change in the dependence of lymphocyte reactivity to phytomitogens on endogenous hormonal factors in experimental bacterial lesion of the prostate]. The relationship between the blast lymphocyte transformation response (BLTR) to PHA P, Con A, PWM and 11-hydroxycorticosteroids (I) and the testosterone (II) levels in the blood plasma and the excretion of 17-ketosteroids with the urine were studied in 11 dogs with experimental urogenous prostatitis, provoked by Staphylococcus aureus, isolated from a patient with chronic prostatitis. Multiple correlation between the BLTR and hormonal factors was noted before the experiment. It was disturbed in a month and restored 2 months after the beginning of the experiment. Before the studies the relationships were mainly realized by direct correlation with 1 and 2 months after the experiment--due to the negative correlation with II. A homeostatic nature of the hormone lymphoid relationship with respect to 11-hydroxy-corticosteroids is suggested."}
{"id": "PMID:465698", "title": "[Effect of lithium chloride on the thyroid gland of white rats].", "content": "The morphological and functional changes in the thyroid tissue of white rats injected lithium chloride at doses 0.5 mekv/kg and 1.0 mekv/kg (groups 1 and 2, respectively) during three weeks were studied by the radiometric, histological and biochemical methods. The radiometric, histological and biochemical methods. The inhibitory action of lithium chloride on hormonogenesis in the thyroid gland and secretion of thyroid hormones into the blood was proved to be directly related to the lithium dose and concentration in the blood. The data of intravital radiometric and morphological analysis of the organs of the 1st group animals suggest some activization of the gland function, with the secretion of the hormones into the blood being suppressed. Increased concentration of the drug inhibits hormonogenesis and secretion of thyroid hormones into the blood.", "contents": "[Effect of lithium chloride on the thyroid gland of white rats]. The morphological and functional changes in the thyroid tissue of white rats injected lithium chloride at doses 0.5 mekv/kg and 1.0 mekv/kg (groups 1 and 2, respectively) during three weeks were studied by the radiometric, histological and biochemical methods. The radiometric, histological and biochemical methods. The inhibitory action of lithium chloride on hormonogenesis in the thyroid gland and secretion of thyroid hormones into the blood was proved to be directly related to the lithium dose and concentration in the blood. The data of intravital radiometric and morphological analysis of the organs of the 1st group animals suggest some activization of the gland function, with the secretion of the hormones into the blood being suppressed. Increased concentration of the drug inhibits hormonogenesis and secretion of thyroid hormones into the blood."}
{"id": "PMID:465699", "title": "[Reparative regeneration of the rabbit cornea with the use of ethanolamine].", "content": "A lesion was induced in the central area of the rabbit cornea as deep as half its thickness, with a trepan 4 mm in diameter. As a stimulant of regeneration, ethanolamine was injected subconjunctivally and subcutaneously. In early stages the control animals showed predominant destructive processes; epithelization was completed on the 15-16th day. A rough leukoma emerged at the deficiency site. Administration of ethanolamine made the proliferative processes in the epithelium and in the ground substance of the cornea start earlier while epithelization was completed on the 10-14th day to form a hardly noteceably leukoma. Ethanolamine has proved to be an adequate stimulant in posttraumatic regeneration of the cornea.", "contents": "[Reparative regeneration of the rabbit cornea with the use of ethanolamine]. A lesion was induced in the central area of the rabbit cornea as deep as half its thickness, with a trepan 4 mm in diameter. As a stimulant of regeneration, ethanolamine was injected subconjunctivally and subcutaneously. In early stages the control animals showed predominant destructive processes; epithelization was completed on the 15-16th day. A rough leukoma emerged at the deficiency site. Administration of ethanolamine made the proliferative processes in the epithelium and in the ground substance of the cornea start earlier while epithelization was completed on the 10-14th day to form a hardly noteceably leukoma. Ethanolamine has proved to be an adequate stimulant in posttraumatic regeneration of the cornea."}
{"id": "PMID:465700", "title": "[Effect of the thymus on hematopoietic stem cells and on their capacity for self-maintenance].", "content": "The hemopoietic cell cloning in the spleen of irradiated mice showed that thymectomy in mature animals produced no significant changes either in the CFU count or in their self-maintenance ability.", "contents": "[Effect of the thymus on hematopoietic stem cells and on their capacity for self-maintenance]. The hemopoietic cell cloning in the spleen of irradiated mice showed that thymectomy in mature animals produced no significant changes either in the CFU count or in their self-maintenance ability."}
{"id": "PMID:465701", "title": "[Histoautoradiographic study of thymidine-3H incorporation into the parenchymal nuclei of the kidneys and other organs in rats with nephrotoxic nephritis].", "content": "The features of changes in the index of thymidine-3H incorporation into the renal parenchyma in nephrotoxic nephritis are compared to other organs. In the kidneys the index of incorporation inthe epithelial cells of the renal cortex was 7.5-fold and in the brain cortex epithelium 15-fold as increased, respectively. The number of labeled glomerular cells was 2.5-fold as increased. In glomerulonephritis, the index of thymidine-3H incorporation in the epithelium of the adrenals, liver, goiter gland and small intestine lowered within 30-80% from normal.", "contents": "[Histoautoradiographic study of thymidine-3H incorporation into the parenchymal nuclei of the kidneys and other organs in rats with nephrotoxic nephritis]. The features of changes in the index of thymidine-3H incorporation into the renal parenchyma in nephrotoxic nephritis are compared to other organs. In the kidneys the index of incorporation inthe epithelial cells of the renal cortex was 7.5-fold and in the brain cortex epithelium 15-fold as increased, respectively. The number of labeled glomerular cells was 2.5-fold as increased. In glomerulonephritis, the index of thymidine-3H incorporation in the epithelium of the adrenals, liver, goiter gland and small intestine lowered within 30-80% from normal."}
{"id": "PMID:465702", "title": "[Autoradiographic analysis of the effect of potassium orotate on the intensity of uridine-5 3H and proline-3H incorporation into wound fibroblasts].", "content": "The intensity of uridine-53H and proline-3H incorporation into fibroblast nuclei and cytoplasm as well as into intercellular spaces was studied in wound healing of mice given potassium orotate and in the control animals. It was shown that proline-3H incorporation was more intensive than that of uridine-53H. At the same time a significant increase in RNA synthesis in fibroblasts under the action of potassium orotate, and a less pronounced rise in proline-containing protein synthesis were noted in similar conditioelationships correlate with the fibroblast ultrastructural changes in the process of their differentiation.", "contents": "[Autoradiographic analysis of the effect of potassium orotate on the intensity of uridine-5 3H and proline-3H incorporation into wound fibroblasts]. The intensity of uridine-53H and proline-3H incorporation into fibroblast nuclei and cytoplasm as well as into intercellular spaces was studied in wound healing of mice given potassium orotate and in the control animals. It was shown that proline-3H incorporation was more intensive than that of uridine-53H. At the same time a significant increase in RNA synthesis in fibroblasts under the action of potassium orotate, and a less pronounced rise in proline-containing protein synthesis were noted in similar conditioelationships correlate with the fibroblast ultrastructural changes in the process of their differentiation."}
{"id": "PMID:465703", "title": "[Morphometric study of the blood-carrying capillaries during the administration of a liquid into the salivary gland].", "content": "The physiological saline was injected into the excretory duct of the cat parotid gland under the pressure of 30, 70 and 120 cm H2O. It was found with the aid of transmission electronic microscopy, morphometry and statistical analysis that the liquid injection into the gland produces compression of the blood capillaries weaving the acinus. The compression of the capillary tubes is shown by a significant reduction in space given to the lumen of the capillaries and their endothelial layer. The compression of the capillary tubes is combined with a two-fold lessening suggest that the additional blood volume entering the gland in response to its perfusion by the liquid does not reach the blood capillaries and is thrown off into the vein vessels through the shunt communications and that regulation of the blood volume getting into the cat parotid gland capillaries is likely to depend on the hydraulic and osmotic state in the interstitial space of glandular lobes.", "contents": "[Morphometric study of the blood-carrying capillaries during the administration of a liquid into the salivary gland]. The physiological saline was injected into the excretory duct of the cat parotid gland under the pressure of 30, 70 and 120 cm H2O. It was found with the aid of transmission electronic microscopy, morphometry and statistical analysis that the liquid injection into the gland produces compression of the blood capillaries weaving the acinus. The compression of the capillary tubes is shown by a significant reduction in space given to the lumen of the capillaries and their endothelial layer. The compression of the capillary tubes is combined with a two-fold lessening suggest that the additional blood volume entering the gland in response to its perfusion by the liquid does not reach the blood capillaries and is thrown off into the vein vessels through the shunt communications and that regulation of the blood volume getting into the cat parotid gland capillaries is likely to depend on the hydraulic and osmotic state in the interstitial space of glandular lobes."}
{"id": "PMID:465704", "title": "[Dynamics of the nuclear DNA content in the liver, skeletal muscles and myocardium of the rat in the postmortem period (a microspectrophotometric study)].", "content": "A comparative microspectrophotometric analysis of changes in nuclear DNA in the liver, skeletal muscle and myocardium of noninbred male albino rats which had died of cerebrocranial injury was conducted during three days following death. A decrease in the content of nuclear DNA within an interval of 30-72 h can be depicted mathematically by regression curves of the type M(t) = e-at+b. The data obtained allowed one to mathematically show the postmortem changes in DNA of the test material.", "contents": "[Dynamics of the nuclear DNA content in the liver, skeletal muscles and myocardium of the rat in the postmortem period (a microspectrophotometric study)]. A comparative microspectrophotometric analysis of changes in nuclear DNA in the liver, skeletal muscle and myocardium of noninbred male albino rats which had died of cerebrocranial injury was conducted during three days following death. A decrease in the content of nuclear DNA within an interval of 30-72 h can be depicted mathematically by regression curves of the type M(t) = e-at+b. The data obtained allowed one to mathematically show the postmortem changes in DNA of the test material."}
{"id": "PMID:465705", "title": "[Characteristics of the development of acute toxic hepatitis in rats stimulated with prodigiozan].", "content": "Acute toxic hepatitis was induced in Wistar rats by means of a single injection of 40% CCl4 on a peach-kernell oil base (0.2 ml/100 g body weight). Under conditions of stimulation with the bacterial polysaccharide prodigiosan, the resistance of hepatocytes to CCl4 sharply increased, which was shown by diminished severity of hepatic parenchyma destruction.", "contents": "[Characteristics of the development of acute toxic hepatitis in rats stimulated with prodigiozan]. Acute toxic hepatitis was induced in Wistar rats by means of a single injection of 40% CCl4 on a peach-kernell oil base (0.2 ml/100 g body weight). Under conditions of stimulation with the bacterial polysaccharide prodigiosan, the resistance of hepatocytes to CCl4 sharply increased, which was shown by diminished severity of hepatic parenchyma destruction."}
{"id": "PMID:465706", "title": "[Structural and immunomorphological characteristics of the human thymus in embryogenesis].", "content": "The thymus glands from one hundred of 4--34-week-old human fetuses were studied by the histologic, histochemical, immunomorphologic and electron microscopic methods. The development of the organ is described from the standpoint of systemogenesis. The laying of the gland is defined at the 5th week of the fetus development, and it reflects the features of the epithelium of the head intestine organs. The differentiation of the reticuloepithelium, the population of the gland by lymphocytes and emergence of antigenic specificity on their surface state at the age of 7--8 weeks. The growth zone of the thymus reticuloepithelium, the significance of Hassal's bodies, the appearance and quantitative dynamics of two subpopulations of T-lymphocytes are described. From 11--12 till 34 weeks of fetal development the percentage of T-lymphocytes forming rosettes with sheep red blood cells virtually does not change (70--90%), while the percentage of lymphocytes forming rosettes with their own red cell increases during the same period from 23 to 70%.", "contents": "[Structural and immunomorphological characteristics of the human thymus in embryogenesis]. The thymus glands from one hundred of 4--34-week-old human fetuses were studied by the histologic, histochemical, immunomorphologic and electron microscopic methods. The development of the organ is described from the standpoint of systemogenesis. The laying of the gland is defined at the 5th week of the fetus development, and it reflects the features of the epithelium of the head intestine organs. The differentiation of the reticuloepithelium, the population of the gland by lymphocytes and emergence of antigenic specificity on their surface state at the age of 7--8 weeks. The growth zone of the thymus reticuloepithelium, the significance of Hassal's bodies, the appearance and quantitative dynamics of two subpopulations of T-lymphocytes are described. From 11--12 till 34 weeks of fetal development the percentage of T-lymphocytes forming rosettes with sheep red blood cells virtually does not change (70--90%), while the percentage of lymphocytes forming rosettes with their own red cell increases during the same period from 23 to 70%."}
{"id": "PMID:465707", "title": "[Reaction of the ganglia to stress effects in the maxillodental area].", "content": "Neurohistologic and histochemical study has been performed of the trigeminal and upper cervical sympathetic ganglia as well as of the nodose ganglion in 35 dogs whose 4 lower teeth were prepared for full metal crowns. It was established that in the polishing of dental hard tissue the ganglia on the prepared side show changes in some neurons, intraganglionic nerve fibers, synaptic endings, in the content and distribution of RNA, glycogen and hyaluronic acid. The maximal evidence of the reaction was noted 1--3 days after operation. The ganglia under study did not show any marked changes following 21--28 days.", "contents": "[Reaction of the ganglia to stress effects in the maxillodental area]. Neurohistologic and histochemical study has been performed of the trigeminal and upper cervical sympathetic ganglia as well as of the nodose ganglion in 35 dogs whose 4 lower teeth were prepared for full metal crowns. It was established that in the polishing of dental hard tissue the ganglia on the prepared side show changes in some neurons, intraganglionic nerve fibers, synaptic endings, in the content and distribution of RNA, glycogen and hyaluronic acid. The maximal evidence of the reaction was noted 1--3 days after operation. The ganglia under study did not show any marked changes following 21--28 days."}
{"id": "PMID:465708", "title": "[Electron microscopic and histochemical study of the localization of adenylate cylase and acetylcholinesterase in the synapses of the rat cerebral cortex and basal ganglia].", "content": "Synapses of the brain cortex and basal ganglia of the rat were studied by means of electron histochemical reactions to adenylate cyclase and acetylcholinesterase. Three types of synapses, viz. cholinergic, adrenergic and mixed, were identified. Typification was carried out on the basis of presynaptic terminal vesicle characteristics and localization of the mentioned enzymes of the synapse receptor area.", "contents": "[Electron microscopic and histochemical study of the localization of adenylate cylase and acetylcholinesterase in the synapses of the rat cerebral cortex and basal ganglia]. Synapses of the brain cortex and basal ganglia of the rat were studied by means of electron histochemical reactions to adenylate cyclase and acetylcholinesterase. Three types of synapses, viz. cholinergic, adrenergic and mixed, were identified. Typification was carried out on the basis of presynaptic terminal vesicle characteristics and localization of the mentioned enzymes of the synapse receptor area."}
{"id": "PMID:465709", "title": "[Role of the catecholaminergic mechanisms and of the caudate nucleus in the development of generalized convulsions caused by the parenteral administration of penicillin].", "content": "In freely moving cats the behavioral and EEG-shifts, accompanied by myoclonic jerks with slow negative waves and spike-wave complexes in the cortexand caudate nucleus, were recorded following a single intramuscular injection of high penicillin doses. The stimulants of catecholaminergic transmission (L-DOPA and apomorphine) inhibited the development of such phenomena but facilitated origination of tonicoclonic cramps. The inhibitors of catecholaminergic synapses (aminazin and haloperidol) exerted reverse effects. The electrolytic injury to the caudate nucleus head also prevented formation of petit mal-like seizures while the threshold low-frequency stimulation of the nucleus increased penicillin effect.", "contents": "[Role of the catecholaminergic mechanisms and of the caudate nucleus in the development of generalized convulsions caused by the parenteral administration of penicillin]. In freely moving cats the behavioral and EEG-shifts, accompanied by myoclonic jerks with slow negative waves and spike-wave complexes in the cortexand caudate nucleus, were recorded following a single intramuscular injection of high penicillin doses. The stimulants of catecholaminergic transmission (L-DOPA and apomorphine) inhibited the development of such phenomena but facilitated origination of tonicoclonic cramps. The inhibitors of catecholaminergic synapses (aminazin and haloperidol) exerted reverse effects. The electrolytic injury to the caudate nucleus head also prevented formation of petit mal-like seizures while the threshold low-frequency stimulation of the nucleus increased penicillin effect."}
{"id": "PMID:465710", "title": "[Restoration of motor functions in disruption of the spinal cord or cauda equina].", "content": "Eight patients with a rupture of the spinal cord at the level of middle thoracic vertebrae or cauda equina at the lumbar level showed partial recovery of motor functions. The development of the activity was revealed by electromyography in body muscles and M. gluteus media in all the patients who attempted voluntary movements. Appearance and development of the activity was also observed in the muscles of the femur and crus. The level of rehabilitation shown by the patients was high enough. They could walk in fixation apparatuses, returned to their occupational activities and could practice self-service. The motor functions recovered more rapidly at the low levels of injury; however, the same and possibly complete rehabilitation was attained in 2 patients with a higher level of injury. The mechanism of the function recovery is underlain by the compensatory development of activity in the muscles of the body and capacity of the distal strip of the spinal cord for elaboration of new motor reactions in which the muscles of the extremities participate.", "contents": "[Restoration of motor functions in disruption of the spinal cord or cauda equina]. Eight patients with a rupture of the spinal cord at the level of middle thoracic vertebrae or cauda equina at the lumbar level showed partial recovery of motor functions. The development of the activity was revealed by electromyography in body muscles and M. gluteus media in all the patients who attempted voluntary movements. Appearance and development of the activity was also observed in the muscles of the femur and crus. The level of rehabilitation shown by the patients was high enough. They could walk in fixation apparatuses, returned to their occupational activities and could practice self-service. The motor functions recovered more rapidly at the low levels of injury; however, the same and possibly complete rehabilitation was attained in 2 patients with a higher level of injury. The mechanism of the function recovery is underlain by the compensatory development of activity in the muscles of the body and capacity of the distal strip of the spinal cord for elaboration of new motor reactions in which the muscles of the extremities participate."}
{"id": "PMID:465711", "title": "[Primary response mechanisms of the adrenal cortex in dogs to pain stress].", "content": "The adrenal cortex of dogs shows a drop in the glucocorticoid content 10--15 sec after the pain action in the presence of the free cholesterol level increase and its esterified form drop. The concentrations of hormones in the blood falls chiefly at the expense of protein bound hydrocortisone. The subsequent phase of the reaction (after 30--60 sec) is characterized by a considerable accumulation of the hormones by the gland. The level of free glucocorticoids significantly increases, the initial ratio of hydrocortisone and cortisone restores, and transcortin depot is filled up. The role of the adrenal and transcortine depot in realization of feedback mechanisms under stress is discussed.", "contents": "[Primary response mechanisms of the adrenal cortex in dogs to pain stress]. The adrenal cortex of dogs shows a drop in the glucocorticoid content 10--15 sec after the pain action in the presence of the free cholesterol level increase and its esterified form drop. The concentrations of hormones in the blood falls chiefly at the expense of protein bound hydrocortisone. The subsequent phase of the reaction (after 30--60 sec) is characterized by a considerable accumulation of the hormones by the gland. The level of free glucocorticoids significantly increases, the initial ratio of hydrocortisone and cortisone restores, and transcortin depot is filled up. The role of the adrenal and transcortine depot in realization of feedback mechanisms under stress is discussed."}
{"id": "PMID:465712", "title": "[Biogenic amine content and the pathomorphological shifts in different organs and tissues in a period of the hematogennic spread and fixation of botulin toxin].", "content": "In experiments on cats it was shown that 30 minutes after intravenous injection of botulinum toxin, type C, there was a fall in the catecholamine and histamine contents with a simultaneous increase of serotonin in various structures of the brain and spinal cord and in a number of inner organs as well. The metabolic changes in the biogenic amines were combined with certain pathomorphologic changes seen in the form of acute swelling, chromatolysis, destruction of some neurons of the spinal cord and brain, distrophic changes in inner organs, and an increased permeability of the blood-tissue barriers. Marked biochemical and pathomorphologic changes in the spinal cord and brain where the minimum concentration of toxin becomes manifest during its spreading allow a conclusion that botulinum neurotoxin shows its pathogenic action through a disturbed metabolism of biologically active substances.", "contents": "[Biogenic amine content and the pathomorphological shifts in different organs and tissues in a period of the hematogennic spread and fixation of botulin toxin]. In experiments on cats it was shown that 30 minutes after intravenous injection of botulinum toxin, type C, there was a fall in the catecholamine and histamine contents with a simultaneous increase of serotonin in various structures of the brain and spinal cord and in a number of inner organs as well. The metabolic changes in the biogenic amines were combined with certain pathomorphologic changes seen in the form of acute swelling, chromatolysis, destruction of some neurons of the spinal cord and brain, distrophic changes in inner organs, and an increased permeability of the blood-tissue barriers. Marked biochemical and pathomorphologic changes in the spinal cord and brain where the minimum concentration of toxin becomes manifest during its spreading allow a conclusion that botulinum neurotoxin shows its pathogenic action through a disturbed metabolism of biologically active substances."}
{"id": "PMID:465713", "title": "[Effects of injurying and restoring the body of rats with microwave (2400 MHz) irradiation].", "content": "In experiments on 2072 rats the thresholds of power density (PD) and the duration of microwave irradiation were obtained with the effect of destruction not more than 0.1%. The speed ratio of formation processes of destruction and regeneration depends on power density of microwave radiation and interpolates the exponential function. Previously obtained (from experiments on mice) and now published data allow the eifference to be established between mice and rats as species. Mice are more sensitive from the standpoint of time of achieving equal effects of destruction, half and full period of regeneration, the speed ratio between destruction and regeneration depending on PD of microwave radiation.", "contents": "[Effects of injurying and restoring the body of rats with microwave (2400 MHz) irradiation]. In experiments on 2072 rats the thresholds of power density (PD) and the duration of microwave irradiation were obtained with the effect of destruction not more than 0.1%. The speed ratio of formation processes of destruction and regeneration depends on power density of microwave radiation and interpolates the exponential function. Previously obtained (from experiments on mice) and now published data allow the eifference to be established between mice and rats as species. Mice are more sensitive from the standpoint of time of achieving equal effects of destruction, half and full period of regeneration, the speed ratio between destruction and regeneration depending on PD of microwave radiation."}
{"id": "PMID:465714", "title": "[Effect of serotonin and histamine on the cellular contractile activity of the internal carotid artery].", "content": "In the experiments on isolated segments of the canine internal carotid artery it was shown that serotonin (5.10(-11)--5.10(-5) g/ml) activated the contractions. Histamine (5.10(-9) g/ml) induced dilation of isolated segments and its higher concentrations produced the contractile responses. Serotonin and histamine were shown to induce the contractions of the depolarized smooth vascular muscle. It is suggested that serotonin activates the inflow of extracellular calcium ions and histamine activates both the inflow of extracellular and the cut-flow of the intracellular calcium ions.", "contents": "[Effect of serotonin and histamine on the cellular contractile activity of the internal carotid artery]. In the experiments on isolated segments of the canine internal carotid artery it was shown that serotonin (5.10(-11)--5.10(-5) g/ml) activated the contractions. Histamine (5.10(-9) g/ml) induced dilation of isolated segments and its higher concentrations produced the contractile responses. Serotonin and histamine were shown to induce the contractions of the depolarized smooth vascular muscle. It is suggested that serotonin activates the inflow of extracellular calcium ions and histamine activates both the inflow of extracellular and the cut-flow of the intracellular calcium ions."}
{"id": "PMID:465715", "title": "[Differences between dose-effect curves for ethanol on locomotion and rearings in mice of various strains].", "content": "Ethanol (1--5 g/kg, per os) dose-response curve for locomotion in male mice of 4 strains (SHR, BALB/c, C3HA and C57BL/6) is characterized by a gradual rise up to the top (at 3 g/kg for all strains except C3HA, in which it was attained at 5 g/kg) and by subsequent decline. The dose-response curve for rearings gradually and gently slopes down to zero. The stimulant effect of ethanol (1.5--3 g/kg) can therefore be measured by an increase in lovomoyion. However, the action of various drugs (or other factors) on this effect has to be evaluated simultaneously from locomotion and rearings since a decrease in locomotion as a result of the curve fracture may indicate both diminution and enhancement of the stimulant effect of ethanol. Simultaneous decrease or increase in the rate of rearings suggests potentiation or diminution of the effect of ethanol, respectively.", "contents": "[Differences between dose-effect curves for ethanol on locomotion and rearings in mice of various strains]. Ethanol (1--5 g/kg, per os) dose-response curve for locomotion in male mice of 4 strains (SHR, BALB/c, C3HA and C57BL/6) is characterized by a gradual rise up to the top (at 3 g/kg for all strains except C3HA, in which it was attained at 5 g/kg) and by subsequent decline. The dose-response curve for rearings gradually and gently slopes down to zero. The stimulant effect of ethanol (1.5--3 g/kg) can therefore be measured by an increase in lovomoyion. However, the action of various drugs (or other factors) on this effect has to be evaluated simultaneously from locomotion and rearings since a decrease in locomotion as a result of the curve fracture may indicate both diminution and enhancement of the stimulant effect of ethanol. Simultaneous decrease or increase in the rate of rearings suggests potentiation or diminution of the effect of ethanol, respectively."}
{"id": "PMID:465716", "title": "[Effect of adrenoblockaders on vascular reactions in sinocarotid baroreflexes].", "content": "Blood pressure in the common carotid artery, the tone of the cerebral and femoral vessels were recorded by resistography in acute experiments on nonanesthetized cats (local anesthetics, listenon) with artificial respiration. The increased pressure in isolated carotid sinuses was accompanied by a decrease in arterial pressure and in the tone of the cerebral and femoral vessels. In both cases the reaction of the cerebral vessels was less pronounced as compared to that shown by the femoral vessels. The blocking of alpha-adrenoreceptors with phentolamine reduced or abolished completely the reactions noted. After beta-adrenergic blockade with obsidan these reactions persisted. The role of carotid baroceptors in the regulation of cerebral circulation under orthostatic collapse is discussed.", "contents": "[Effect of adrenoblockaders on vascular reactions in sinocarotid baroreflexes]. Blood pressure in the common carotid artery, the tone of the cerebral and femoral vessels were recorded by resistography in acute experiments on nonanesthetized cats (local anesthetics, listenon) with artificial respiration. The increased pressure in isolated carotid sinuses was accompanied by a decrease in arterial pressure and in the tone of the cerebral and femoral vessels. In both cases the reaction of the cerebral vessels was less pronounced as compared to that shown by the femoral vessels. The blocking of alpha-adrenoreceptors with phentolamine reduced or abolished completely the reactions noted. After beta-adrenergic blockade with obsidan these reactions persisted. The role of carotid baroceptors in the regulation of cerebral circulation under orthostatic collapse is discussed."}
{"id": "PMID:465717", "title": "[Effect of leucine-enkephalin on the neuronal activity of the cerebral cortex].", "content": "In the experiments on non-anesthetised, flaxedilimmobilized rats it has been shown that the injection of leucin-enkephalin (150 micrograms) into the lateral ventricle of the rat brain induces different changes in the activity of 21 test neurons of the sensomotor zone of the brain cortex. Spike discharges of 5 neurons decreased, while those of 11 neurons increased. Naloxone (2 mg/kg, i. v.) completely prevented both inhibiting and activating effects of leucin-enkephalin after repeated injections of this pentapeptide. Leucin-enkephalin failed to change the activity of 5 neurons.", "contents": "[Effect of leucine-enkephalin on the neuronal activity of the cerebral cortex]. In the experiments on non-anesthetised, flaxedilimmobilized rats it has been shown that the injection of leucin-enkephalin (150 micrograms) into the lateral ventricle of the rat brain induces different changes in the activity of 21 test neurons of the sensomotor zone of the brain cortex. Spike discharges of 5 neurons decreased, while those of 11 neurons increased. Naloxone (2 mg/kg, i. v.) completely prevented both inhibiting and activating effects of leucin-enkephalin after repeated injections of this pentapeptide. Leucin-enkephalin failed to change the activity of 5 neurons."}
{"id": "PMID:465718", "title": "[Role of calcium in the mechanism of the change in vascular reactivity under the action of reserpine].", "content": "Reserpine induces an appreciable reduction of the calcium content in the segments of the distal aorta and rabbit femoral arteries incubated in a saline. These changes develop simultaneously to those in the reaction pattern of the vascular segment to direct electric stimulation (decline of constrictor responses and their subsequent replacement by relaxation). It is postulated that the decreased calcium content in the vascular wall may be one of the causes of altered vascular reactivity.", "contents": "[Role of calcium in the mechanism of the change in vascular reactivity under the action of reserpine]. Reserpine induces an appreciable reduction of the calcium content in the segments of the distal aorta and rabbit femoral arteries incubated in a saline. These changes develop simultaneously to those in the reaction pattern of the vascular segment to direct electric stimulation (decline of constrictor responses and their subsequent replacement by relaxation). It is postulated that the decreased calcium content in the vascular wall may be one of the causes of altered vascular reactivity."}
{"id": "PMID:465719", "title": "[Reversible endocytosis apparatus of the blood platelets].", "content": "The functioning of ganules of blood platelets participating in the reversible endocytosis reaction was investigated in rabbits. Eczocytosis was shown to be initiated by inducers of two types: proteolytic enzymes and compounds accumulated by the granules (biogenic amines, dyes, anesthetics and phenothiazines). Heparin inhibits thrombin-induced eczocytosis. During eczocytosis produced by accumulation of compounds by blood platelet granules, the cells lose their capacity for maintaining the aggregation state. The nature of blood platelet granules participating in the reversible endocytosis reaction is discussed.", "contents": "[Reversible endocytosis apparatus of the blood platelets]. The functioning of ganules of blood platelets participating in the reversible endocytosis reaction was investigated in rabbits. Eczocytosis was shown to be initiated by inducers of two types: proteolytic enzymes and compounds accumulated by the granules (biogenic amines, dyes, anesthetics and phenothiazines). Heparin inhibits thrombin-induced eczocytosis. During eczocytosis produced by accumulation of compounds by blood platelet granules, the cells lose their capacity for maintaining the aggregation state. The nature of blood platelet granules participating in the reversible endocytosis reaction is discussed."}
{"id": "PMID:465720", "title": "[Glycosaminoglycans of the aorta in avitaminosis].", "content": "It has been shown that pregnancy specific beta 1-globulin (SBG) is found in the rat blood serum on the 6--7th day of pregnancy. SBG reaches the maximum by the end of pregnancy and is not detected already at the 3d--4th day after delivery. The placenta is the site of the SBG snythesis. Other organs of the pregnant rat are not able to incorporate radioactive aminoacids into the protein in vitro.", "contents": "[Glycosaminoglycans of the aorta in avitaminosis]. It has been shown that pregnancy specific beta 1-globulin (SBG) is found in the rat blood serum on the 6--7th day of pregnancy. SBG reaches the maximum by the end of pregnancy and is not detected already at the 3d--4th day after delivery. The placenta is the site of the SBG snythesis. Other organs of the pregnant rat are not able to incorporate radioactive aminoacids into the protein in vitro."}
{"id": "PMID:465721", "title": "[Effects of injurying and restoring the body of mice with microwave (2400 MHz) irradiation].", "content": "In the experiments on 2200 mice the thresholds of powder density (PD) and time of irradiation were defined, when the death level did not exceed 0.1%. Dependence between the speed ratio of the destruction/regeneration and PD approached the exponential function. The functional dependences obtained quantitatively characterize the adaptive abilities of the mouse organism exposed to microwave irradiation.", "contents": "[Effects of injurying and restoring the body of mice with microwave (2400 MHz) irradiation]. In the experiments on 2200 mice the thresholds of powder density (PD) and time of irradiation were defined, when the death level did not exceed 0.1%. Dependence between the speed ratio of the destruction/regeneration and PD approached the exponential function. The functional dependences obtained quantitatively characterize the adaptive abilities of the mouse organism exposed to microwave irradiation."}
{"id": "PMID:465722", "title": "[Change in the activity of specific and nonspecific immunity mechanisms in vitamin B1 deficiency].", "content": "The effect of thiamin deficiency on the immune response and activity of some mechanisms of natural immunity was studied in experimental mature rats. Thiamin deficiency was simulated by a single injection of hydroxythiamin (thiamin antagonists). Hydroxythiamin markedly decreased the complement activity, phagocytic activity of peripheral blood leucocytes, serum bactericidal activity as well as antibody production in response to sheep red blood cells. On the contrary, lysozyme activity increased. Vitamin B1 deficiency reduced 14C-leucine incorporation activity in the liver proteins.", "contents": "[Change in the activity of specific and nonspecific immunity mechanisms in vitamin B1 deficiency]. The effect of thiamin deficiency on the immune response and activity of some mechanisms of natural immunity was studied in experimental mature rats. Thiamin deficiency was simulated by a single injection of hydroxythiamin (thiamin antagonists). Hydroxythiamin markedly decreased the complement activity, phagocytic activity of peripheral blood leucocytes, serum bactericidal activity as well as antibody production in response to sheep red blood cells. On the contrary, lysozyme activity increased. Vitamin B1 deficiency reduced 14C-leucine incorporation activity in the liver proteins."}
{"id": "PMID:465723", "title": "[Morphological study of the neurosecretory hypothalamo-hypophyseal system and thyroid gland in intestinal carcinogenesis].", "content": "Pathomorphology of the neurosecretory hypothalamo-hypophyseal system and the thyroid gland of 150 rats of both sexes was investigated in intestinal carcinogenesis induced by 1.2-dimethyl hydrazine. Inhibition of the neurosecretory activity of paraventricular and supraoptical nuclei as well as atrophic changes in th: thyroid gland were found to be associated with the latent period of carcinogenesis. The arising of the intestinal tumor is accompanied by hypertrophy of neurons and their nuclei, by a decrease in the neurosecretory substance content and the thyroid gland tendency to return to normal. The tumor spreading provoked neuron hypertrophy and the reduction of the neurosecretory substance as well and pronounced atrophic changes in the thyroid gland.", "contents": "[Morphological study of the neurosecretory hypothalamo-hypophyseal system and thyroid gland in intestinal carcinogenesis]. Pathomorphology of the neurosecretory hypothalamo-hypophyseal system and the thyroid gland of 150 rats of both sexes was investigated in intestinal carcinogenesis induced by 1.2-dimethyl hydrazine. Inhibition of the neurosecretory activity of paraventricular and supraoptical nuclei as well as atrophic changes in th: thyroid gland were found to be associated with the latent period of carcinogenesis. The arising of the intestinal tumor is accompanied by hypertrophy of neurons and their nuclei, by a decrease in the neurosecretory substance content and the thyroid gland tendency to return to normal. The tumor spreading provoked neuron hypertrophy and the reduction of the neurosecretory substance as well and pronounced atrophic changes in the thyroid gland."}
{"id": "PMID:465724", "title": "[Physiological and reparative regeneration of the mitochondria].", "content": "The ultrastructure of mitochondria of hepatocytes in normal and pathological conditions was studied. It was shown that the process of regeneration of the ultrastructure of swollen mitochondria with a lucent matrix up to the normal state was completed in hepatocytes of the rat and chick embryos within one day. It was established that one of the ways of intraorganoid regeneration of mitochondria in hepatocytes of chick embryos and of mice after injections of CCl4 twice a week for 5 months was clasmatosis of the destroyed mitochondria fragments and their removal through the partially disintegrated exterior membrane of mitochondria followed by the membrane restoration. The process of mitochondrial regeneration after clasmatosis of its fragments was shown to require two days in the chick embryo hepatocytes.", "contents": "[Physiological and reparative regeneration of the mitochondria]. The ultrastructure of mitochondria of hepatocytes in normal and pathological conditions was studied. It was shown that the process of regeneration of the ultrastructure of swollen mitochondria with a lucent matrix up to the normal state was completed in hepatocytes of the rat and chick embryos within one day. It was established that one of the ways of intraorganoid regeneration of mitochondria in hepatocytes of chick embryos and of mice after injections of CCl4 twice a week for 5 months was clasmatosis of the destroyed mitochondria fragments and their removal through the partially disintegrated exterior membrane of mitochondria followed by the membrane restoration. The process of mitochondrial regeneration after clasmatosis of its fragments was shown to require two days in the chick embryo hepatocytes."}
{"id": "PMID:465725", "title": "[Effect of repeated cooling on the state of the adrenals and corneal epithelial proliferation in white rats].", "content": "The effect of repeated cooling on the adrenal glands, on the mitosis and the number of DNA-synthesising cells in the corneal epithelium of albino rats was studied. The animals were cooled by the contact method to 28 degrees C and were exposed to this temperature for an hour daily within 5 days. Strong activation of the suprarenal glands was observed: 2-fold increase in the suprarenal weight, 3-fold decrease in cholesterol content, 4-fold growth of 11-hydroxy-corticosteroid level in the blood and enhanced adrenaline excretion. The average number of mitoses in the corneal decreases 2-fold. Depression was not associated with the change in mitosis rate but with the inhibition of the cell entrance into mitosis in interphase. The level of pathological mitoses did not change. Chronic stress was not accompanied by the change in the number of DNA-synthesising cells or intensity of DNA-symthesis.", "contents": "[Effect of repeated cooling on the state of the adrenals and corneal epithelial proliferation in white rats]. The effect of repeated cooling on the adrenal glands, on the mitosis and the number of DNA-synthesising cells in the corneal epithelium of albino rats was studied. The animals were cooled by the contact method to 28 degrees C and were exposed to this temperature for an hour daily within 5 days. Strong activation of the suprarenal glands was observed: 2-fold increase in the suprarenal weight, 3-fold decrease in cholesterol content, 4-fold growth of 11-hydroxy-corticosteroid level in the blood and enhanced adrenaline excretion. The average number of mitoses in the corneal decreases 2-fold. Depression was not associated with the change in mitosis rate but with the inhibition of the cell entrance into mitosis in interphase. The level of pathological mitoses did not change. Chronic stress was not accompanied by the change in the number of DNA-synthesising cells or intensity of DNA-symthesis."}
{"id": "PMID:465726", "title": "[Interaction of mitogenic lectins with lymphocyte plasma membranes].", "content": "It is shown that mitogenic stimuli from concanavalin A and phytohemagglutinin are summed up in the time. Under given experimental conditions each of the mitogens used did not induce mitogenesis when the interaction with lymphocytes continued for 14 and/or 20 hours. The results obtained are discussed from the point of view of the cell-to-cell interaction between the lymphocytes. It is assumed that the ability of the stimuli to sum up in the time underlies the nonspecific mechanism of defense from tolerance.", "contents": "[Interaction of mitogenic lectins with lymphocyte plasma membranes]. It is shown that mitogenic stimuli from concanavalin A and phytohemagglutinin are summed up in the time. Under given experimental conditions each of the mitogens used did not induce mitogenesis when the interaction with lymphocytes continued for 14 and/or 20 hours. The results obtained are discussed from the point of view of the cell-to-cell interaction between the lymphocytes. It is assumed that the ability of the stimuli to sum up in the time underlies the nonspecific mechanism of defense from tolerance."}
{"id": "PMID:465727", "title": "[Effect of short-term cerebral ischemia on the microcirculation in the rat mesentery].", "content": "The effect of brain ischemia, produced by constriction of the common carotid arteries, on microcirculation in the mesentery was studied in experiments on rats. The spreading and intensity of microcirculatory disturbances were dependent on duration of ischemia and post-ischemia. The state of systemic hemodynamics and microcirculation in the mesentery was compared. Possible mechanisms of the microcirculatory disturbances are discussed.", "contents": "[Effect of short-term cerebral ischemia on the microcirculation in the rat mesentery]. The effect of brain ischemia, produced by constriction of the common carotid arteries, on microcirculation in the mesentery was studied in experiments on rats. The spreading and intensity of microcirculatory disturbances were dependent on duration of ischemia and post-ischemia. The state of systemic hemodynamics and microcirculation in the mesentery was compared. Possible mechanisms of the microcirculatory disturbances are discussed."}
{"id": "PMID:465728", "title": "[Effect of armin on the ultrastructure of the neuromuscular synapse].", "content": "Alterations induced by the cholinesterase inhibitor armin (5.10(-7) g/ml) in the ultrastructure of motor nerve endings of the rat phrenic diaphragmal preparations at rest or electric stimulation of the nerve were studied. It was shown that armin at rest induced ultrastructural lesions in the endings similar to those in the control preparations during nerve stimulation. Electric stimulation did not produce additional changes in the ultrastructure of the neuromuscular junction under armin action. It is suggested that the disorder of the nerve ending function may be of importance in the mechanism of the blocking action of armin on the neuromuscular transmission.", "contents": "[Effect of armin on the ultrastructure of the neuromuscular synapse]. Alterations induced by the cholinesterase inhibitor armin (5.10(-7) g/ml) in the ultrastructure of motor nerve endings of the rat phrenic diaphragmal preparations at rest or electric stimulation of the nerve were studied. It was shown that armin at rest induced ultrastructural lesions in the endings similar to those in the control preparations during nerve stimulation. Electric stimulation did not produce additional changes in the ultrastructure of the neuromuscular junction under armin action. It is suggested that the disorder of the nerve ending function may be of importance in the mechanism of the blocking action of armin on the neuromuscular transmission."}
{"id": "PMID:465729", "title": "[Electron microscopic study of the process of the removal of autogenous blood erythocytes beyond the limits of the subdural space].", "content": "The internal plate of the dura mater and arachnoidea of the dob brain was studied by electron microscopy after introducing 0.3--0.5 ml of the autogenous blood into the subdural space. The arachnoidea of the excretory channels and structural elements of the internal plate of the dura mater (cells of the internal covering layer, the collagen fibril and microfibril layer, the wall of the blood capillaries of the internal capillary network) involve the morphological substratum of the liquor-blood barrier-I between the liquor and blood of the blood capillaries of the internal capillary network of the dura mater. It was established that red cells of the subdural space penetrate the thickness of the dura mater, where they concentrate around blood capillaries of the internal capillary network, rather than penetrate into the arachnoidea.", "contents": "[Electron microscopic study of the process of the removal of autogenous blood erythocytes beyond the limits of the subdural space]. The internal plate of the dura mater and arachnoidea of the dob brain was studied by electron microscopy after introducing 0.3--0.5 ml of the autogenous blood into the subdural space. The arachnoidea of the excretory channels and structural elements of the internal plate of the dura mater (cells of the internal covering layer, the collagen fibril and microfibril layer, the wall of the blood capillaries of the internal capillary network) involve the morphological substratum of the liquor-blood barrier-I between the liquor and blood of the blood capillaries of the internal capillary network of the dura mater. It was established that red cells of the subdural space penetrate the thickness of the dura mater, where they concentrate around blood capillaries of the internal capillary network, rather than penetrate into the arachnoidea."}
{"id": "PMID:465730", "title": "Neutrophil kinetics in chronic neutropenia.", "content": "Quantitative studies of bone marrow neutrophil pool sizes and production rates and of blood neutrophil kinetics were performed in 16 patients with chronic neutropenia without splenomegaly. Marrow netrophil cellularity was determined from a ferrokinetic estimate of marrow normoblasts and from neutrophil-erythroid ratios determined from marrow sections. Postmitotic pool turnover was derived from the postmitotic pool size and transit time, the latter determined from 3H-thymidine neutrophil emergence time. Blood neutrophil kinetics were studied with 32P-diisopropylfluoophosphate-labeled autologous neutrophils. Mitotic pool size was basal or below basal in 12 of the 16 patients. The turnover of the post-mitotic neutrophils was subbasal in 6, basal in 7, and above basal in 3 patients. Blood neutrophil turnover was within the normal range in 8 patients and decreased in 8. The degree of ineffective granulocytopoiesis was assessed by comparing the relative size of the mitotic pool, postmitotic pool turnover, and blood turnover. On this basis, 13 of the 16 patients showed appreciable degrees of ineffective granulocytopoiesis. Ineffective neutrophil production occurred both early and late in neutrophil development. These studies indicate that most patients with chronic neutropenia without splenomegaly lack a proliferative marrow response to the neutropenia and suggest that ineffective granulocytopoiesis is a common feature of this disorder.", "contents": "Neutrophil kinetics in chronic neutropenia. Quantitative studies of bone marrow neutrophil pool sizes and production rates and of blood neutrophil kinetics were performed in 16 patients with chronic neutropenia without splenomegaly. Marrow netrophil cellularity was determined from a ferrokinetic estimate of marrow normoblasts and from neutrophil-erythroid ratios determined from marrow sections. Postmitotic pool turnover was derived from the postmitotic pool size and transit time, the latter determined from 3H-thymidine neutrophil emergence time. Blood neutrophil kinetics were studied with 32P-diisopropylfluoophosphate-labeled autologous neutrophils. Mitotic pool size was basal or below basal in 12 of the 16 patients. The turnover of the post-mitotic neutrophils was subbasal in 6, basal in 7, and above basal in 3 patients. Blood neutrophil turnover was within the normal range in 8 patients and decreased in 8. The degree of ineffective granulocytopoiesis was assessed by comparing the relative size of the mitotic pool, postmitotic pool turnover, and blood turnover. On this basis, 13 of the 16 patients showed appreciable degrees of ineffective granulocytopoiesis. Ineffective neutrophil production occurred both early and late in neutrophil development. These studies indicate that most patients with chronic neutropenia without splenomegaly lack a proliferative marrow response to the neutropenia and suggest that ineffective granulocytopoiesis is a common feature of this disorder."}
{"id": "PMID:465731", "title": "ABO compatibility and platelet transfusions of alloimmunized thrombocytopenic patients.", "content": "With data on 91 alloimmunized thrombocytopenic patients and 389 donor-recipient pairs matched or selectively mismatched for HLA antigens, it was observed that ABO incompatibility significantly reduced the effectiveness of platelet transfusions. The mean 24-hr recovery of platelets from histocompatible donors and from donors selectively mismatched for cross-reactive HLA antigens was decreased by approximately 23% if the donor typed for blood group A and/or B not found in the recipient. Thus, the reduction in platelet recovery associated with ABO incompatibility is not of a magnitude that would contraindicate transfusion of ABO-mismatched platelets.", "contents": "ABO compatibility and platelet transfusions of alloimmunized thrombocytopenic patients. With data on 91 alloimmunized thrombocytopenic patients and 389 donor-recipient pairs matched or selectively mismatched for HLA antigens, it was observed that ABO incompatibility significantly reduced the effectiveness of platelet transfusions. The mean 24-hr recovery of platelets from histocompatible donors and from donors selectively mismatched for cross-reactive HLA antigens was decreased by approximately 23% if the donor typed for blood group A and/or B not found in the recipient. Thus, the reduction in platelet recovery associated with ABO incompatibility is not of a magnitude that would contraindicate transfusion of ABO-mismatched platelets."}
{"id": "PMID:465732", "title": "A case of generalized amyloidosis associated with cyclic neutropenia.", "content": "A case of generalized amyloidosis associated with cyclic neutropenia is presented. A 24-yr-old female with cyclic neutropenia died from intestinal obstruction caused by necrosis and perforation of the small intestine. Post-mortem examination revealed generalized amyloidosis involving almost all organs. Amyloid deposits were prominent, especially in the alimentary tract, kidneys, spleen, and small blood vessels. As has been suggested in gray collie dogs with congenital cyclic neutropenia known to develop secondary amyloidosis in adulthood, an increase of antigenic stimulation during the intermittent bouts of acute infections may be one of the factors responsible for the development of secondary amyloidosis in this case. Although the association of amyloidosis and cyclic neutropenia in man has rarely been described, it is probable that amyloidosis is not a rare complication of human cyclic neutropenia, considering that patients with this hematologic disorder are chronically exposed to excessive antigenic stimulation.", "contents": "A case of generalized amyloidosis associated with cyclic neutropenia. A case of generalized amyloidosis associated with cyclic neutropenia is presented. A 24-yr-old female with cyclic neutropenia died from intestinal obstruction caused by necrosis and perforation of the small intestine. Post-mortem examination revealed generalized amyloidosis involving almost all organs. Amyloid deposits were prominent, especially in the alimentary tract, kidneys, spleen, and small blood vessels. As has been suggested in gray collie dogs with congenital cyclic neutropenia known to develop secondary amyloidosis in adulthood, an increase of antigenic stimulation during the intermittent bouts of acute infections may be one of the factors responsible for the development of secondary amyloidosis in this case. Although the association of amyloidosis and cyclic neutropenia in man has rarely been described, it is probable that amyloidosis is not a rare complication of human cyclic neutropenia, considering that patients with this hematologic disorder are chronically exposed to excessive antigenic stimulation."}
{"id": "PMID:465733", "title": "Membrane receptors and their redistribution in lymphoproliferative disorders.", "content": "Lymphoid cells from 20 patients with lymphoproliferative disorders, including chronic lymphocytic leukemia, hairy cell leukemia, Sezary syndrome, lymphoma, and lymphadenitis, were studied for redistribution of surface membrane immunoglobulins (SmIg) and concanavalin A (Con-A) receptors. Fluorescein-labeled polyvalent goat anti-human immunoglobulin and fluoresceinated concanavalin A were used as ligands. Results were similar with both ligands. The highest percentage of capping of ligand-membrane receptors was noted in mononuclear cells from patients with \"hairy\" cell leukemia: from 24% to 90%. These cells showed moderate to marked fluorescein activity and were able to cap within 15 min at 4 degrees C. Chronic lymphocytic leukemia cells showed a weak fluorescein stain with a very low percentage of cells (0%--16%) capping. Lymph node cells from patients with lymphoma demonstrated moderate to strong fluorescein activity with only an average of 3% of the cells capping; while lymphoid cells from patients with lymphaedenitis showed an average of 27.5% capping and moderate fluorescein activity. Capping of Con-A receptors in mononuclear cells from patients with Sezary syndrome was poor (0%--14%) with moderate fluorescein intensity. This report demonstrates difference in density and mobility of binding sites for SmIg and Con-A on the surface membrane of lymphoid cells from various subclasses of lymphoproliferative disorders. These differences may assist in the differential diagnosis and classification of these conditions.", "contents": "Membrane receptors and their redistribution in lymphoproliferative disorders. Lymphoid cells from 20 patients with lymphoproliferative disorders, including chronic lymphocytic leukemia, hairy cell leukemia, Sezary syndrome, lymphoma, and lymphadenitis, were studied for redistribution of surface membrane immunoglobulins (SmIg) and concanavalin A (Con-A) receptors. Fluorescein-labeled polyvalent goat anti-human immunoglobulin and fluoresceinated concanavalin A were used as ligands. Results were similar with both ligands. The highest percentage of capping of ligand-membrane receptors was noted in mononuclear cells from patients with \"hairy\" cell leukemia: from 24% to 90%. These cells showed moderate to marked fluorescein activity and were able to cap within 15 min at 4 degrees C. Chronic lymphocytic leukemia cells showed a weak fluorescein stain with a very low percentage of cells (0%--16%) capping. Lymph node cells from patients with lymphoma demonstrated moderate to strong fluorescein activity with only an average of 3% of the cells capping; while lymphoid cells from patients with lymphaedenitis showed an average of 27.5% capping and moderate fluorescein activity. Capping of Con-A receptors in mononuclear cells from patients with Sezary syndrome was poor (0%--14%) with moderate fluorescein intensity. This report demonstrates difference in density and mobility of binding sites for SmIg and Con-A on the surface membrane of lymphoid cells from various subclasses of lymphoproliferative disorders. These differences may assist in the differential diagnosis and classification of these conditions."}
{"id": "PMID:465734", "title": "The effect of aggregation and release on platelet prothrombin-converting activity.", "content": "Platelets provide a procoagulant activity for the conversion of prothrombin to thrombin during normal hemostatis. This activity designated as platelet prothrombin-converting activity (PPCA) was monitored as rate of thrombin production in a two-stage assay using gel-filtered bovine platelets, factor Xa, and prothrombin. Expression of PPCA was not associated with ADP-induced release or platelet shape change but was associated with aggregation. Release of the contents of dense bodies, measured by release of 14C-5-hydroxytryptamine, was not required for expression of PPCA during platelet aggregation. During the PPCA assay, 5-hydroxytrypamine was released, but only after onset of thrombin production. Furthermore, the release of 5-hydroxytryptamine was retarded during the assay by the addition of 2 mM theophylline and 100 nM prostaglandin E1 without a comparable reduction in PPCA. In addition, 125I-factor-Xa was bound in greater amounts to platelets (aspirin-treated) after ADP-induced aggregation (without detectable release) than to unactivated control platelets. Finally, the PPCA of the ADP-activated platelets was saturated with respect to factors Xa and Va at less than 1 nM concentrations, indicating that the aggregation induced by ADP leads to the exposure of specific procoagulant sites by some process other than dense body secretion.", "contents": "The effect of aggregation and release on platelet prothrombin-converting activity. Platelets provide a procoagulant activity for the conversion of prothrombin to thrombin during normal hemostatis. This activity designated as platelet prothrombin-converting activity (PPCA) was monitored as rate of thrombin production in a two-stage assay using gel-filtered bovine platelets, factor Xa, and prothrombin. Expression of PPCA was not associated with ADP-induced release or platelet shape change but was associated with aggregation. Release of the contents of dense bodies, measured by release of 14C-5-hydroxytryptamine, was not required for expression of PPCA during platelet aggregation. During the PPCA assay, 5-hydroxytrypamine was released, but only after onset of thrombin production. Furthermore, the release of 5-hydroxytryptamine was retarded during the assay by the addition of 2 mM theophylline and 100 nM prostaglandin E1 without a comparable reduction in PPCA. In addition, 125I-factor-Xa was bound in greater amounts to platelets (aspirin-treated) after ADP-induced aggregation (without detectable release) than to unactivated control platelets. Finally, the PPCA of the ADP-activated platelets was saturated with respect to factors Xa and Va at less than 1 nM concentrations, indicating that the aggregation induced by ADP leads to the exposure of specific procoagulant sites by some process other than dense body secretion."}
{"id": "PMID:465735", "title": "Fetal erythropoiesis and hemoglobin ontogeny in tail-short (Ts/+) mutant mice.", "content": "Mutant Ts/+ fetuses are developmentally retarded as compared to normal +/+ littermates. Mutant fetuses have less total hemoglobin than do normal fetuses of the same gestational age. However, when compared to +/+ fetuses of similar body weight, Ts/+ fetuses have the appropriate amount of total hemoglobin, suggesting that the apparent anemia observed in mutant fetuses is most likely the result of delay in growth and development. Changes in proportions of embryonic hemoglobins during fetal development are similar in Ts/+ and +/+ fetuses at day 12 and later of gestation. Moreover, adult hemoglobin is detected in circulating primitive nucleated erythrocytes in the developmentally retarded Ts/+ mutant fetuses at about the same chronologic age as their +/+ normal littermates.", "contents": "Fetal erythropoiesis and hemoglobin ontogeny in tail-short (Ts/+) mutant mice. Mutant Ts/+ fetuses are developmentally retarded as compared to normal +/+ littermates. Mutant fetuses have less total hemoglobin than do normal fetuses of the same gestational age. However, when compared to +/+ fetuses of similar body weight, Ts/+ fetuses have the appropriate amount of total hemoglobin, suggesting that the apparent anemia observed in mutant fetuses is most likely the result of delay in growth and development. Changes in proportions of embryonic hemoglobins during fetal development are similar in Ts/+ and +/+ fetuses at day 12 and later of gestation. Moreover, adult hemoglobin is detected in circulating primitive nucleated erythrocytes in the developmentally retarded Ts/+ mutant fetuses at about the same chronologic age as their +/+ normal littermates."}
{"id": "PMID:465736", "title": "The hemostatic imbalance of plasma-exchange transfusion.", "content": "Plasma exchange has been proposed as a treatment for multiple disorders. Three patients with amyotropic lateral sclerosis, who were hemostatically normal, were studied through a total of 11 4-liter exchanges. Plasma was replaced by an equal volume of 5% albumin or 5% plasma protein fraction. Serial studies revealed that immediately after the exchange transfusion, there was significant prolongation of the prothrombin, partial thromboplastin, and thrombin times with reduction of the fibrinogen and antithrombin III levels. Factors V, VII-X, IX, and X were all significantly decreased, as were the factor VIII antigen, procoagulant, and the ristocetin cofactor activities. Platelet counts were obtained before and after exchanges and revealed significant decreases. Four hours after exchange, all parameters remained abnormal except the factor IX, ristocetin cofactor, and factor VIII procoagulant activities. By 24 hr, all hemostatic parameters had returned to normal. These studies indicate that plasma-exchange transfusion with material devoid of coagulation factors results in a coagulation defect that may be of clinical significance in a hemostatically compromised patient.", "contents": "The hemostatic imbalance of plasma-exchange transfusion. Plasma exchange has been proposed as a treatment for multiple disorders. Three patients with amyotropic lateral sclerosis, who were hemostatically normal, were studied through a total of 11 4-liter exchanges. Plasma was replaced by an equal volume of 5% albumin or 5% plasma protein fraction. Serial studies revealed that immediately after the exchange transfusion, there was significant prolongation of the prothrombin, partial thromboplastin, and thrombin times with reduction of the fibrinogen and antithrombin III levels. Factors V, VII-X, IX, and X were all significantly decreased, as were the factor VIII antigen, procoagulant, and the ristocetin cofactor activities. Platelet counts were obtained before and after exchanges and revealed significant decreases. Four hours after exchange, all parameters remained abnormal except the factor IX, ristocetin cofactor, and factor VIII procoagulant activities. By 24 hr, all hemostatic parameters had returned to normal. These studies indicate that plasma-exchange transfusion with material devoid of coagulation factors results in a coagulation defect that may be of clinical significance in a hemostatically compromised patient."}
{"id": "PMID:465737", "title": "Antibody-mediated acquired sideroblastic anemia: response to cytotoxic therapy.", "content": "A 5 1/2-yr-old child developed severe anemia with erythroid hypoplasia and 50% ringed sideroblasts in his bone marrow. A serum inhibitor of erythropoiesis was demonstrated, utilizing syngeneic and autologous bone marrow in a plasma-clot culture system. The IgG fraction of the patient's serum effected similar suppression of erythroid colony formation. Prednisone therapy was ineffective, but following treatment with cyclophosphamide, normal erythropoiesis was established, ringed sideroblasts disappeared, and his serum no longer inhibited erythropoiesis in vitro. Cyclophosphamide was discontinued, and the patient has remained hematologically normal. This patient is an example of antibody-mediated sideroblastic anemia successfully treated with a cytotoxic drug.", "contents": "Antibody-mediated acquired sideroblastic anemia: response to cytotoxic therapy. A 5 1/2-yr-old child developed severe anemia with erythroid hypoplasia and 50% ringed sideroblasts in his bone marrow. A serum inhibitor of erythropoiesis was demonstrated, utilizing syngeneic and autologous bone marrow in a plasma-clot culture system. The IgG fraction of the patient's serum effected similar suppression of erythroid colony formation. Prednisone therapy was ineffective, but following treatment with cyclophosphamide, normal erythropoiesis was established, ringed sideroblasts disappeared, and his serum no longer inhibited erythropoiesis in vitro. Cyclophosphamide was discontinued, and the patient has remained hematologically normal. This patient is an example of antibody-mediated sideroblastic anemia successfully treated with a cytotoxic drug."}
{"id": "PMID:465738", "title": "Immune suppression of erythropoiesis in transient erythroblastopenia of childhood.", "content": "Serum and IgG from four children with transient erythroblastopenia of childhood (TEC) was tested to see what effect it would have on development of erythroid colonies from bone marrow mononuclear cells. Serum and IgG specimens obtained at the time of diagnosis uniformly suppressed erythroid colony development from CFU-E. Washed bone marrow mononuclear cells from a child with TEC failed to grow in the presence of his own serum, but grew normally in the presence of isologous serum. Serum specimens obtained from patients after recovery from TEC had no effect on erythroid colony development. The anemia of TEC appears to be due to transient immune suppression of erythroid colony development.", "contents": "Immune suppression of erythropoiesis in transient erythroblastopenia of childhood. Serum and IgG from four children with transient erythroblastopenia of childhood (TEC) was tested to see what effect it would have on development of erythroid colonies from bone marrow mononuclear cells. Serum and IgG specimens obtained at the time of diagnosis uniformly suppressed erythroid colony development from CFU-E. Washed bone marrow mononuclear cells from a child with TEC failed to grow in the presence of his own serum, but grew normally in the presence of isologous serum. Serum specimens obtained from patients after recovery from TEC had no effect on erythroid colony development. The anemia of TEC appears to be due to transient immune suppression of erythroid colony development."}
{"id": "PMID:465739", "title": "Leukopheresis therapy of leukemic reticuloendotheliosis (hairy cell leukemia).", "content": "Intensive leukopheresis has been valuable in the short-term palliation of chronic lymphocytic and granulocytic leukemias. A 47-yr-old man with refractory leukemic reticuloendotheliosis (hairy cell leukemia) manifested by anemia, thrombocytopenia, elevated peripheral leukemia cell counts, generalized lymph node enlargement, and leukemic infiltrative skin disease was treated with serial leukopheresis. Removal of approximately 7 X 10(11) peripheral leukemia cells resulted in marked clinical and hematologic improvement with resolution of enlarged lymph nodes and clearing of skin infiltrates. At the time of this reporting, more than 400 wk since the last leukopheresis, the patient continues to do well. The improvement in all blood counts, reduction in lymph node size, and clearing of skin lesions paralleled the reduction of peripheral leukemia cell load by leukopheresis, suggesting mobilization of leukemia cells from marrow, lymph nodes, and skin. Removal of large numbers of leukemia cells in hairy cell leukemia has the potential of achieving sustained clinical improvement and may be a useful alternative therapy for these patients.", "contents": "Leukopheresis therapy of leukemic reticuloendotheliosis (hairy cell leukemia). Intensive leukopheresis has been valuable in the short-term palliation of chronic lymphocytic and granulocytic leukemias. A 47-yr-old man with refractory leukemic reticuloendotheliosis (hairy cell leukemia) manifested by anemia, thrombocytopenia, elevated peripheral leukemia cell counts, generalized lymph node enlargement, and leukemic infiltrative skin disease was treated with serial leukopheresis. Removal of approximately 7 X 10(11) peripheral leukemia cells resulted in marked clinical and hematologic improvement with resolution of enlarged lymph nodes and clearing of skin infiltrates. At the time of this reporting, more than 400 wk since the last leukopheresis, the patient continues to do well. The improvement in all blood counts, reduction in lymph node size, and clearing of skin lesions paralleled the reduction of peripheral leukemia cell load by leukopheresis, suggesting mobilization of leukemia cells from marrow, lymph nodes, and skin. Removal of large numbers of leukemia cells in hairy cell leukemia has the potential of achieving sustained clinical improvement and may be a useful alternative therapy for these patients."}
{"id": "PMID:465741", "title": "Improvement of the red blood cell O2 release capacity by lipid vesicle-mediated incorporation of inositol hexaphosphate.", "content": "Fusion of unilamellar lipid vesicles with red blood cells has been used to deliver entrapped inositol hexaphosphate (IHP) to the cells. Incorporated IHP tightly bound to hemoglobin produces a significant and lasting \"right-shift\" of the O2-binding curve of red blood cells. The O2 release capacity of red blood cells is increased by this method up to 270% of the normal value. The Bohr effect is increased so that carbon dioxide transport is also enhanced. The fine structure of the erythrocytes does not seem to be altered by the lipid vesicle incorporation.", "contents": "Improvement of the red blood cell O2 release capacity by lipid vesicle-mediated incorporation of inositol hexaphosphate. Fusion of unilamellar lipid vesicles with red blood cells has been used to deliver entrapped inositol hexaphosphate (IHP) to the cells. Incorporated IHP tightly bound to hemoglobin produces a significant and lasting \"right-shift\" of the O2-binding curve of red blood cells. The O2 release capacity of red blood cells is increased by this method up to 270% of the normal value. The Bohr effect is increased so that carbon dioxide transport is also enhanced. The fine structure of the erythrocytes does not seem to be altered by the lipid vesicle incorporation."}
{"id": "PMID:465742", "title": "Remission induction and remission maintenance in adult acute nonlymphocytic leukemia employing a modified cytostatic (COAP) regimen.", "content": "Thirty adult patients suffering from acute nonlymphocytic leukemia (ANLL) were treated according to a modified COAP regimen. Vincristine, cyclophosphamide, and prednisone were given by push injection, while cytosine arabinoside was infused over periods of 8 h. Nineteen patients (63%) achieved complete remission. Remission maintenance therapy consisted of 6-mercaptopurine daily and methotrexate twice weekly. Later in the study, COAP consolidation and reinduction was added, which improved the median duration of complete remission from 7 to 24 months. Comparison of the results with the literature shows that the modified COAP regimen is one of the most effective treatment schedules for adult ANLL.", "contents": "Remission induction and remission maintenance in adult acute nonlymphocytic leukemia employing a modified cytostatic (COAP) regimen. Thirty adult patients suffering from acute nonlymphocytic leukemia (ANLL) were treated according to a modified COAP regimen. Vincristine, cyclophosphamide, and prednisone were given by push injection, while cytosine arabinoside was infused over periods of 8 h. Nineteen patients (63%) achieved complete remission. Remission maintenance therapy consisted of 6-mercaptopurine daily and methotrexate twice weekly. Later in the study, COAP consolidation and reinduction was added, which improved the median duration of complete remission from 7 to 24 months. Comparison of the results with the literature shows that the modified COAP regimen is one of the most effective treatment schedules for adult ANLL."}
{"id": "PMID:465744", "title": "[Antibody screening of commercially available immunoglobulins. Erythrocyte-, HLA- and autoantibodies (author's transl)].", "content": "A great number of commercially available immunoglobulins was screened for erythrocyte-, HLA- and autoantibodies. Isoagglutinin titers were in general low, hemolysins were not detected. No preparation was found to contain detectable amounts of autoantibodies versus nuclei, mitochondria, smooth muscle or parietal cells. Yet 7 out of 22 immunoglobulin charges contained irregular red cell antibodies, aside of polyspecific albumin reactive types in 5 cases a distinct anti-D. Only the preparation examined in 1975 contained polyspecific HLA-antibodies. 1977/78 all preparations for intravenous use were free from undesired antibodies, while those for intramuscular application were contaminated with irregular red cell antibodies 1975 as well as 1978. Because of their low strength these antibodies imply no clinical risk. Nonetheless an improvement of the official rules for the production and control of immunoglobulin preparations is inevitable to avoid damage caused by exceptional charges with extremely strong antibodies of this type.", "contents": "[Antibody screening of commercially available immunoglobulins. Erythrocyte-, HLA- and autoantibodies (author's transl)]. A great number of commercially available immunoglobulins was screened for erythrocyte-, HLA- and autoantibodies. Isoagglutinin titers were in general low, hemolysins were not detected. No preparation was found to contain detectable amounts of autoantibodies versus nuclei, mitochondria, smooth muscle or parietal cells. Yet 7 out of 22 immunoglobulin charges contained irregular red cell antibodies, aside of polyspecific albumin reactive types in 5 cases a distinct anti-D. Only the preparation examined in 1975 contained polyspecific HLA-antibodies. 1977/78 all preparations for intravenous use were free from undesired antibodies, while those for intramuscular application were contaminated with irregular red cell antibodies 1975 as well as 1978. Because of their low strength these antibodies imply no clinical risk. Nonetheless an improvement of the official rules for the production and control of immunoglobulin preparations is inevitable to avoid damage caused by exceptional charges with extremely strong antibodies of this type."}
{"id": "PMID:465745", "title": "[Value and interest of fine needle aspiration cytology in the follow-up of irradiated breast cancers (author's transl)].", "content": "Between 1968 and 1975, the authors studied at the Institut Curie the value of fine needle aspiration cytology in the follow-up of breast cancers, which have been previously treated with definitive irradiation (182 cases) or lumpectomy and post-operative irradiation (20 cases). The cytologic results have been compared to the pathologic aspects of the surgical specimens. The cytologic procedure was made when the tumor decrease was incomplete or too slow, or when, after a free interval, was noted in the irradiated breast a tissue reaction clinically and radiologically indefinite. The post-therapeutic investigation interested 202 cases of breast carcinoma. Compared to the histologic result, the cytologic results proved to be: -- identical. . . . . . . in 170 cases (84%) -- false benign . . . . . in 4 cases (2%) -- false malignant . . . .  in 4 cases (2%) -- insufficient cell material in 24 cases (12%) The high rate of correct cytologic diagnosis and the very low rate of wrong results prove the value and interest of the fine needle aspiration cytology for residual tumor masses or for recurrences in irradiated breast cancers.", "contents": "[Value and interest of fine needle aspiration cytology in the follow-up of irradiated breast cancers (author's transl)]. Between 1968 and 1975, the authors studied at the Institut Curie the value of fine needle aspiration cytology in the follow-up of breast cancers, which have been previously treated with definitive irradiation (182 cases) or lumpectomy and post-operative irradiation (20 cases). The cytologic results have been compared to the pathologic aspects of the surgical specimens. The cytologic procedure was made when the tumor decrease was incomplete or too slow, or when, after a free interval, was noted in the irradiated breast a tissue reaction clinically and radiologically indefinite. The post-therapeutic investigation interested 202 cases of breast carcinoma. Compared to the histologic result, the cytologic results proved to be: -- identical. . . . . . . in 170 cases (84%) -- false benign . . . . . in 4 cases (2%) -- false malignant . . . .  in 4 cases (2%) -- insufficient cell material in 24 cases (12%) The high rate of correct cytologic diagnosis and the very low rate of wrong results prove the value and interest of the fine needle aspiration cytology for residual tumor masses or for recurrences in irradiated breast cancers."}
{"id": "PMID:465746", "title": "[Immunological aspects of interactions between mother and foetus (author's transl)].", "content": "The essential information about materno-foetal immunologic interactions is reviewed. After a brief description of placental tissue (decidual cells from uterine mucosa, trophoblast), evidence that immunisation can be achieved by introduction of antigen into normal uterus is presented, although grafts performed in uterus of pregnant females are prolonged. Throphoblast cells bear specific antigens but histocompatibility antigens are usually not detectable at their surface. Proteins and cells from the foetus enter the maternal circulation and vice-versa, resulting in the production of antibodies against cell-associated foetal antigens by the mother. Some regulatory process of the maternal immune response have been investigated including blocking antibodies, maternal and foetal suppressor T cells. The biological roles of trophoblast and that of sustained microchimerism are underlined. Immunologic damage to the foetus can be produced only by inducing a cytotoxic cellular response before pregnancy. Possible analogies between immune responses to foetal allografts and tumors are discussed.", "contents": "[Immunological aspects of interactions between mother and foetus (author's transl)]. The essential information about materno-foetal immunologic interactions is reviewed. After a brief description of placental tissue (decidual cells from uterine mucosa, trophoblast), evidence that immunisation can be achieved by introduction of antigen into normal uterus is presented, although grafts performed in uterus of pregnant females are prolonged. Throphoblast cells bear specific antigens but histocompatibility antigens are usually not detectable at their surface. Proteins and cells from the foetus enter the maternal circulation and vice-versa, resulting in the production of antibodies against cell-associated foetal antigens by the mother. Some regulatory process of the maternal immune response have been investigated including blocking antibodies, maternal and foetal suppressor T cells. The biological roles of trophoblast and that of sustained microchimerism are underlined. Immunologic damage to the foetus can be produced only by inducing a cytotoxic cellular response before pregnancy. Possible analogies between immune responses to foetal allografts and tumors are discussed."}
{"id": "PMID:465747", "title": "[Placental metastasis (author's transl)].", "content": "Rarity of placental metastasis is only apparent, for only few placentas of cancerous mothers have been examined histologically. However, it may show biological and immunological conditions which are characteristics of foeto-placental unit. During metastatic spread of solid tumors or hematologic malignancies in the mother, tumor emboli may be localized in intervillous spaces, without being real placental metastasis. Rarely tumor emboli are able to invade the struma of chorionic villi and produce true placental metastases: twelve such observations have been published, seven of which were malignant melanomas. It is even more exceptional that metastatic spread reaches the foetus. In most of the cases, it is thus protected against maternal cancer. This historical observation holds true. The fear of transplacental graft to the foetus is not an argument favorable of terminating a cancer associated pregnancy and foetal metastasis of maternal origin are not among the causes of congenital cancers in children.", "contents": "[Placental metastasis (author's transl)]. Rarity of placental metastasis is only apparent, for only few placentas of cancerous mothers have been examined histologically. However, it may show biological and immunological conditions which are characteristics of foeto-placental unit. During metastatic spread of solid tumors or hematologic malignancies in the mother, tumor emboli may be localized in intervillous spaces, without being real placental metastasis. Rarely tumor emboli are able to invade the struma of chorionic villi and produce true placental metastases: twelve such observations have been published, seven of which were malignant melanomas. It is even more exceptional that metastatic spread reaches the foetus. In most of the cases, it is thus protected against maternal cancer. This historical observation holds true. The fear of transplacental graft to the foetus is not an argument favorable of terminating a cancer associated pregnancy and foetal metastasis of maternal origin are not among the causes of congenital cancers in children."}
{"id": "PMID:465748", "title": "[Epithelial displasias of the uterine cervix during pregnancy and its evolution (author's transl)].", "content": "During the ten year routing study of smears in 12,300 pregnant women, 180 cases of atypias of the uterine cervix were detected (1.46%). However, only 106 of these had sufficient follow up to allow us to draw useful conclusions. The lesions detected included: 1 invasive carcinoma, 71 atypical epithelial lesions and 34 border lesions of which 20 (58%) showed improvement and 14 (41%) remained unchanged. A comparative sutdy of 11,090 sexually active women who were on high oestrogen combination pills, revealed a similar incidence of cervical lesions (1.27%). The lesions included: 2 invasive carcinomas, 100 epithelial atypias and 38 border lesions of which 44 per cent showed improvement and 55 per cent remained unchanged. Cervical lesions were detected in only 0.5 per cent of women on law oestrogen combination pills. These results indicate that it is almost impossible for the cytologist to arrive at a diagnosis of carcinoma in situ in the pregnant woman or in the woman on the pill. No morphologic guide exists to help establish a prognosis. Only follow up study of the cervical lesion is valuable in this regard. Any lesion remaining 6 to 12 months after the delivery should be removed.", "contents": "[Epithelial displasias of the uterine cervix during pregnancy and its evolution (author's transl)]. During the ten year routing study of smears in 12,300 pregnant women, 180 cases of atypias of the uterine cervix were detected (1.46%). However, only 106 of these had sufficient follow up to allow us to draw useful conclusions. The lesions detected included: 1 invasive carcinoma, 71 atypical epithelial lesions and 34 border lesions of which 20 (58%) showed improvement and 14 (41%) remained unchanged. A comparative sutdy of 11,090 sexually active women who were on high oestrogen combination pills, revealed a similar incidence of cervical lesions (1.27%). The lesions included: 2 invasive carcinomas, 100 epithelial atypias and 38 border lesions of which 44 per cent showed improvement and 55 per cent remained unchanged. Cervical lesions were detected in only 0.5 per cent of women on law oestrogen combination pills. These results indicate that it is almost impossible for the cytologist to arrive at a diagnosis of carcinoma in situ in the pregnant woman or in the woman on the pill. No morphologic guide exists to help establish a prognosis. Only follow up study of the cervical lesion is valuable in this regard. Any lesion remaining 6 to 12 months after the delivery should be removed."}
{"id": "PMID:465749", "title": "[Fetal risk of cancer chemotherapy (author's transl)].", "content": "The risks of embryonic, fetal, gondal damage of cancer chemotherapy are reviewed. Contrasting with the numerous malformations seen in laboratory animals, the teratogenic risk is low in man. Methotrexate is really dangerous during the first trimester of pregnancy. In malignant haematological diseases and solid tumours, the prognosis of the disease is the essential target but the use of immuno-suppressive drugs in non-malignant diseases is hazardous before 40 years of age. All the investigations show that alkylating agents injure the gonads. Young women should be avised to use contraceptives. The future of children born after administration of anti cancer drugs is uncertain. Sterility, carcinogenic risk, mutation, teratogenetic effects in future generations cannot be ruled out.", "contents": "[Fetal risk of cancer chemotherapy (author's transl)]. The risks of embryonic, fetal, gondal damage of cancer chemotherapy are reviewed. Contrasting with the numerous malformations seen in laboratory animals, the teratogenic risk is low in man. Methotrexate is really dangerous during the first trimester of pregnancy. In malignant haematological diseases and solid tumours, the prognosis of the disease is the essential target but the use of immuno-suppressive drugs in non-malignant diseases is hazardous before 40 years of age. All the investigations show that alkylating agents injure the gonads. Young women should be avised to use contraceptives. The future of children born after administration of anti cancer drugs is uncertain. Sterility, carcinogenic risk, mutation, teratogenetic effects in future generations cannot be ruled out."}
{"id": "PMID:465750", "title": "[Fertility and the progeny of children surviving cancer treatment (author's transl)].", "content": "Little is known about the late effects of damage to the gonads in children treated for cancer. This investigation requires a prolonged surveillance. Radiotherapy sterilizes the ovaries above a dose of about 500 rads in an adult. Therefore, to protect at least one ovary in partial irradiation of a child's abdomen in mandatory whenever possible, either by restriction of the irradiated volume, or transposition of the ovary. Combined chemotherapies do not seem to inhibit endocrine function but it is too early to appreciate the effects of different combinations of drugs on reproductive function in girls. In boys, alkylating drugs cause an oligo or azoospermia, either temporary or permanently. The genetic risks in the descendants of children treated for cancer are still unknown.", "contents": "[Fertility and the progeny of children surviving cancer treatment (author's transl)]. Little is known about the late effects of damage to the gonads in children treated for cancer. This investigation requires a prolonged surveillance. Radiotherapy sterilizes the ovaries above a dose of about 500 rads in an adult. Therefore, to protect at least one ovary in partial irradiation of a child's abdomen in mandatory whenever possible, either by restriction of the irradiated volume, or transposition of the ovary. Combined chemotherapies do not seem to inhibit endocrine function but it is too early to appreciate the effects of different combinations of drugs on reproductive function in girls. In boys, alkylating drugs cause an oligo or azoospermia, either temporary or permanently. The genetic risks in the descendants of children treated for cancer are still unknown."}
{"id": "PMID:465751", "title": "[Invasive carcinoma of the uterine cervix associated with pregnancy (author's transl)].", "content": "In 4,396 invasive cervix carcinomas treated between 1934 and 1973, 52 occurred during pregnancy or within 3 months post partum. Stages I were more frequent among pregnant women (24%) than without pregnancy (8%) and stages II were as frequent (50%). Stages I and II were more frequent among pregnant women treated during 1963-1973 than 1934-1962. The 3 years' survival rate, all stages combined, for pregnant women (42%) was lower than for matched control non-pregnant women (62%). This difference was significant at P = 0.05, but at 5 and 10 years, the difference was not significant for patients treated between 1963-1973. At an equal stage, survival rate seemed lower for stages IIb and III (P = 0.02 for these two stages combined.) The lymph node involvement was not more frequent; the decrease of global survival rate could be associated to some factors and especially incomplete therapy and an under-estimation of the tumor extension because of the pregnancy. Our results are compared to the data literature and therapeutic schedules are discussed.", "contents": "[Invasive carcinoma of the uterine cervix associated with pregnancy (author's transl)]. In 4,396 invasive cervix carcinomas treated between 1934 and 1973, 52 occurred during pregnancy or within 3 months post partum. Stages I were more frequent among pregnant women (24%) than without pregnancy (8%) and stages II were as frequent (50%). Stages I and II were more frequent among pregnant women treated during 1963-1973 than 1934-1962. The 3 years' survival rate, all stages combined, for pregnant women (42%) was lower than for matched control non-pregnant women (62%). This difference was significant at P = 0.05, but at 5 and 10 years, the difference was not significant for patients treated between 1963-1973. At an equal stage, survival rate seemed lower for stages IIb and III (P = 0.02 for these two stages combined.) The lymph node involvement was not more frequent; the decrease of global survival rate could be associated to some factors and especially incomplete therapy and an under-estimation of the tumor extension because of the pregnancy. Our results are compared to the data literature and therapeutic schedules are discussed."}
{"id": "PMID:465835", "title": "The development and evaluation of a small ready-reference library collection for a rural practice: a case study.", "content": "A demonstration core collection of twenty-four ready-reference sources and five journals was selected cooperatively by a solo practitioner in rural Menifee County, Kentucky, and the University of Kentucky Medical Center Library Field Librarian to fit the ready-reference and current awareness information needs of a primary care solo rural practice in eastern Kentucky. The collection selections were systematically assessed by the physician to determine their utility in filling the practitioner's information needs with regard to his particular situation in terms of medical experience and level of training, available library and educational resources, and the type of health problems seen in his practice. This assessment showed that the Rural Demonstration Library Collection completely filled the information needs of the physician 66% of the time materials were consulted and filled his immediate information needs either completely or partially 82% of the time the collection was searched. This demonstration has shown that, under a specific set of circumstances, a librarian and a solo rural practitioner can effectively work together in identifying health sciences materials which fit the information needs of a solo rural practitioner. It suggests that cooperation between a librarian and a physician is important in meeting information needs.", "contents": "The development and evaluation of a small ready-reference library collection for a rural practice: a case study. A demonstration core collection of twenty-four ready-reference sources and five journals was selected cooperatively by a solo practitioner in rural Menifee County, Kentucky, and the University of Kentucky Medical Center Library Field Librarian to fit the ready-reference and current awareness information needs of a primary care solo rural practice in eastern Kentucky. The collection selections were systematically assessed by the physician to determine their utility in filling the practitioner's information needs with regard to his particular situation in terms of medical experience and level of training, available library and educational resources, and the type of health problems seen in his practice. This assessment showed that the Rural Demonstration Library Collection completely filled the information needs of the physician 66% of the time materials were consulted and filled his immediate information needs either completely or partially 82% of the time the collection was searched. This demonstration has shown that, under a specific set of circumstances, a librarian and a solo rural practitioner can effectively work together in identifying health sciences materials which fit the information needs of a solo rural practitioner. It suggests that cooperation between a librarian and a physician is important in meeting information needs."}
{"id": "PMID:465836", "title": "Classification of support staff in a consortium medical library: a case study.", "content": "A representative committee of Houston Academy of Medicine-Texas Medical Center Library staff and faculty, under the direction of the library administration, successfully redesigned a job classification system for the library's nonprofessional staff. In the new system all nonprofessionals are assigned to one of five grade levels, each with a corresponding salary range. To determine its appropriate grade level each job is analyzed and assigned a numerical value using a point system based on a set of five factors, each of which is assigned a relative number of points. The factors used to measure jobs are: education and experience, complexity of work, administrative accountability, manual skill, and contact with users. Each factor is described according to degrees, so that a job can be given partial credit for a factor. An advisory staff classification committee now participates in the ongoing administration of the classification system.", "contents": "Classification of support staff in a consortium medical library: a case study. A representative committee of Houston Academy of Medicine-Texas Medical Center Library staff and faculty, under the direction of the library administration, successfully redesigned a job classification system for the library's nonprofessional staff. In the new system all nonprofessionals are assigned to one of five grade levels, each with a corresponding salary range. To determine its appropriate grade level each job is analyzed and assigned a numerical value using a point system based on a set of five factors, each of which is assigned a relative number of points. The factors used to measure jobs are: education and experience, complexity of work, administrative accountability, manual skill, and contact with users. Each factor is described according to degrees, so that a job can be given partial credit for a factor. An advisory staff classification committee now participates in the ongoing administration of the classification system."}
{"id": "PMID:465837", "title": "MLA Certification by examination under the 1964 code: a survey of 1975-1976 participants.", "content": "The increased attention being given to an examination in the Medical Library Association certification process prompted a study of a group taking the examination under the old (1964 revision) code. Completed questionnaires were received from fifty-three of the sixty-five persons who took the examination in 1975 and 1976. Backgrounds of individuals varied, especially in years of experience. The majority of those responding had worked in both health sciences and other libraries. Experience in health sciences libraries was reported by 92% of those replying. A number of reasons for seeking MLA certification were given. Problems in being able to take MLA-approved courses prompted the choice of the examination process. Preparation for the examination was chiefly by self-directed study. Continuing education courses were considered as of greater value for professional development than as help on the examination. The examination was rated \"moderately difficult\" by 57% of those responding. Opinions were expressed on the skills and areas of expertise which were considered by this group to be important in an examination for professional competence.", "contents": "MLA Certification by examination under the 1964 code: a survey of 1975-1976 participants. The increased attention being given to an examination in the Medical Library Association certification process prompted a study of a group taking the examination under the old (1964 revision) code. Completed questionnaires were received from fifty-three of the sixty-five persons who took the examination in 1975 and 1976. Backgrounds of individuals varied, especially in years of experience. The majority of those responding had worked in both health sciences and other libraries. Experience in health sciences libraries was reported by 92% of those replying. A number of reasons for seeking MLA certification were given. Problems in being able to take MLA-approved courses prompted the choice of the examination process. Preparation for the examination was chiefly by self-directed study. Continuing education courses were considered as of greater value for professional development than as help on the examination. The examination was rated \"moderately difficult\" by 57% of those responding. Opinions were expressed on the skills and areas of expertise which were considered by this group to be important in an examination for professional competence."}
{"id": "PMID:465865", "title": "Mechanism of inhibition of prostaglandin biosynthesis by hydrocortisone in rat leucocytes.", "content": "Hydrocortisone (10 microgram/ml) greatly inhibits the prostaglandin release by rat peritoneal leucocytes phagocytosing killed bacteria. The inhibition, which occurs after an initial latency of 30 min, is completely reversed by either actinomycin D (0.5 microgram/ml) or cycloheximide (1 microgram/ml). Since these antibiotics are known inhibitors of DNA-dependent RNA synthesis and protein synthesis respectively, it appears that the mechanism of inhibition of prostaglandin biosynthesis by hydrocortisone in rat leucocytes involves stimulation of transcription and induction of protein synthesis.", "contents": "Mechanism of inhibition of prostaglandin biosynthesis by hydrocortisone in rat leucocytes. Hydrocortisone (10 microgram/ml) greatly inhibits the prostaglandin release by rat peritoneal leucocytes phagocytosing killed bacteria. The inhibition, which occurs after an initial latency of 30 min, is completely reversed by either actinomycin D (0.5 microgram/ml) or cycloheximide (1 microgram/ml). Since these antibiotics are known inhibitors of DNA-dependent RNA synthesis and protein synthesis respectively, it appears that the mechanism of inhibition of prostaglandin biosynthesis by hydrocortisone in rat leucocytes involves stimulation of transcription and induction of protein synthesis."}
{"id": "PMID:465866", "title": "The effect of intracerebroventricular administration of methionine-enkephalin on the stress-induced secretion of corticosterone in mice.", "content": "Ether stress or intracerebroventricular (i.c.v.) administration of saline, met-enkephalin, or naltrexone raised plasma corticosterone levels in mice. The response to ether stress was abolished by a preceding ether stress or by pretreatment with i.c.v. saline or naltrexone. However, following i.c.v. met-enkephalin, plasma corticosterone was significantly elevated by ether stress, the effect being blocked by simultaneous injection of met-enkephalin and naltrexone. Met-enkephalin appears to prevent fast-feedback inhibition of the hypothalamus-pituitary-adrenal system.", "contents": "The effect of intracerebroventricular administration of methionine-enkephalin on the stress-induced secretion of corticosterone in mice. Ether stress or intracerebroventricular (i.c.v.) administration of saline, met-enkephalin, or naltrexone raised plasma corticosterone levels in mice. The response to ether stress was abolished by a preceding ether stress or by pretreatment with i.c.v. saline or naltrexone. However, following i.c.v. met-enkephalin, plasma corticosterone was significantly elevated by ether stress, the effect being blocked by simultaneous injection of met-enkephalin and naltrexone. Met-enkephalin appears to prevent fast-feedback inhibition of the hypothalamus-pituitary-adrenal system."}
{"id": "PMID:465867", "title": "Pharmacological effects of phosphatidylserine liposomes: regulation of gylcolysis and energy level in brain.", "content": "1 The accumulation of glucose in the brain produced by the administration of phosphatidylserine liposomes into mice has been studied by measurement of the cerebral contents of glycolytic intermediates and high-energy compounds. 2 With a normal supply of oxygen to the brain, inhibition of glycolysis is indicated mainly at the phosphofructokinase step. The ratio of glucose-6-phosphate to fructose-1,6-diphosphate increased, whereas the levels of pyruvate and especially lactate decreased. 3 Under conditions of cerebral ischaemia, the administration of phosphatidylserine delays glycogen mobilization and ATP use. As a consequence of decreased energy utilization, the brain adenylate energy charge remains at a high level. 4 It is concluded that the phosphatidylserine-induced glucose accumulation in the brain is due to reduced energy expenditure and therefore to a decrease in carbohydrate consumption. The inhibition of glycolysis by the high level of adenylate energy charge is probably the control mechanism explaining the decreased carbohydrate utilization.", "contents": "Pharmacological effects of phosphatidylserine liposomes: regulation of gylcolysis and energy level in brain. 1 The accumulation of glucose in the brain produced by the administration of phosphatidylserine liposomes into mice has been studied by measurement of the cerebral contents of glycolytic intermediates and high-energy compounds. 2 With a normal supply of oxygen to the brain, inhibition of glycolysis is indicated mainly at the phosphofructokinase step. The ratio of glucose-6-phosphate to fructose-1,6-diphosphate increased, whereas the levels of pyruvate and especially lactate decreased. 3 Under conditions of cerebral ischaemia, the administration of phosphatidylserine delays glycogen mobilization and ATP use. As a consequence of decreased energy utilization, the brain adenylate energy charge remains at a high level. 4 It is concluded that the phosphatidylserine-induced glucose accumulation in the brain is due to reduced energy expenditure and therefore to a decrease in carbohydrate consumption. The inhibition of glycolysis by the high level of adenylate energy charge is probably the control mechanism explaining the decreased carbohydrate utilization."}
{"id": "PMID:465868", "title": "Stimulation and inhibition of the sodium pump by cardioactive steroids in relation to their binding sites and their inotropic effect on guinea-pig isolated atria.", "content": "1 The actions of ouabain, ouabagenin and dihydroouabain on the contractility and on the ionic content have been investigated in left guinea-pig atria stimulated at 3.3 Hz. The specific binding of ouabain and its displacement by the other cardenolides have been determined. 2 The action of either ouabain or ouabagenin on Na and K content was qualitatively different according to the concentration employed. Low doses evoked a reduction of Nai whereas high doses produced an increase. Dihydroouabain evoked only a Nai gain. 3 The increase of KCl concentration from 2.7 to 12 mM decreased Nai in untreated atria and displaced ouabain dose-effect curves to the right. 4 ED50 values for the positive inotropic effect were lower than ED50 values for the inhibition of the pump and were not similarly affected by an increase in KCl concentration. 5 The specific binding of ouabain occurred at high and low affinity sites, related to Na pump stimulation and inhibition respectively. 6 The increase in KCl reduced the affinity of the two groups of sites for ouabain and increased the capacity of the high-affinity sites whereas the capacity of the other sites remained unchanged. 7 The results confirm the existence of two specific binding sites for ouabain in guinea-pig heart and suggest that the inhibition of the Na pump is not the only mechanism responsible for the positive inotropic effect of cardiac glycosides.", "contents": "Stimulation and inhibition of the sodium pump by cardioactive steroids in relation to their binding sites and their inotropic effect on guinea-pig isolated atria. 1 The actions of ouabain, ouabagenin and dihydroouabain on the contractility and on the ionic content have been investigated in left guinea-pig atria stimulated at 3.3 Hz. The specific binding of ouabain and its displacement by the other cardenolides have been determined. 2 The action of either ouabain or ouabagenin on Na and K content was qualitatively different according to the concentration employed. Low doses evoked a reduction of Nai whereas high doses produced an increase. Dihydroouabain evoked only a Nai gain. 3 The increase of KCl concentration from 2.7 to 12 mM decreased Nai in untreated atria and displaced ouabain dose-effect curves to the right. 4 ED50 values for the positive inotropic effect were lower than ED50 values for the inhibition of the pump and were not similarly affected by an increase in KCl concentration. 5 The specific binding of ouabain occurred at high and low affinity sites, related to Na pump stimulation and inhibition respectively. 6 The increase in KCl reduced the affinity of the two groups of sites for ouabain and increased the capacity of the high-affinity sites whereas the capacity of the other sites remained unchanged. 7 The results confirm the existence of two specific binding sites for ouabain in guinea-pig heart and suggest that the inhibition of the Na pump is not the only mechanism responsible for the positive inotropic effect of cardiac glycosides."}
{"id": "PMID:465869", "title": "Actions of prostaglandin precursors and other unsaturated fatty acids on conduction time and refractory period in the cat heart in situ.", "content": "1 The effect of arachidonic, dihomo-gamma-linolenic, linoleic, alpha-linolenic and oleic acid, given by intravenous infusion, on conduction time and functional refractory period have been studied in the cat heart in situ. 2 The prostaglandin precursors, arachidonic acid and dihomo-gamma-linolenic acid, prolonged the conduction time and the functional refractory period. Linoleic acid was also effective but to a lesser degree. alpha-Linolenic acid and oleic acid showed no or only a weak effect in thie respect. 3 Pretreatment was indomethacin diminished or abolished the actions of the three effective fatty acids but not those of prostaglandin E2. 4 The results suggest that the effects of prostaglandin prostaglandin precursors on conduction time and refractory period are responsible for their antiarrhythmic effectiveness and that these effects are attributable to their endogenous conversion into prostaglandins.", "contents": "Actions of prostaglandin precursors and other unsaturated fatty acids on conduction time and refractory period in the cat heart in situ. 1 The effect of arachidonic, dihomo-gamma-linolenic, linoleic, alpha-linolenic and oleic acid, given by intravenous infusion, on conduction time and functional refractory period have been studied in the cat heart in situ. 2 The prostaglandin precursors, arachidonic acid and dihomo-gamma-linolenic acid, prolonged the conduction time and the functional refractory period. Linoleic acid was also effective but to a lesser degree. alpha-Linolenic acid and oleic acid showed no or only a weak effect in thie respect. 3 Pretreatment was indomethacin diminished or abolished the actions of the three effective fatty acids but not those of prostaglandin E2. 4 The results suggest that the effects of prostaglandin prostaglandin precursors on conduction time and refractory period are responsible for their antiarrhythmic effectiveness and that these effects are attributable to their endogenous conversion into prostaglandins."}
{"id": "PMID:465870", "title": "Characterization of ultraviolet light-induced relaxation of the isolated duodenum of the rat.", "content": "1 Isolated duodenum of the rat, exposed to ultraviolet (u.v.) light in the presence of NO2 ions, responded with reversible relaxation. 2 The photorelaxation response did not seem to involve any known receptor mechanisms and was independent of any ganglionic or neuronal influences. 3 Changes in the ionic environment of the tissue showed that NA+ and Ca2+ were essential for the photorelaxation. K+ depolarized-tissue did not show the photoresponse. 4 The presence of the metabolic inhibitors, iodoacetic acid, 2,4-dinitrophenol, sodium fluoride, sodium azide or potassium cyanide, abolished the photorelaxation response. 5 It is proposed that the photorelaxation of the tissue resulted from the liberation of metabolic energy following NO2 ion-dependent absorption of u.v. light energy, which in turn, interfered with the Na+ ion movement across the cell membrane.", "contents": "Characterization of ultraviolet light-induced relaxation of the isolated duodenum of the rat. 1 Isolated duodenum of the rat, exposed to ultraviolet (u.v.) light in the presence of NO2 ions, responded with reversible relaxation. 2 The photorelaxation response did not seem to involve any known receptor mechanisms and was independent of any ganglionic or neuronal influences. 3 Changes in the ionic environment of the tissue showed that NA+ and Ca2+ were essential for the photorelaxation. K+ depolarized-tissue did not show the photoresponse. 4 The presence of the metabolic inhibitors, iodoacetic acid, 2,4-dinitrophenol, sodium fluoride, sodium azide or potassium cyanide, abolished the photorelaxation response. 5 It is proposed that the photorelaxation of the tissue resulted from the liberation of metabolic energy following NO2 ion-dependent absorption of u.v. light energy, which in turn, interfered with the Na+ ion movement across the cell membrane."}
{"id": "PMID:465871", "title": "The effects of H1- and H2-receptor agonists and antagonists on total lung resistance, dynamic lung compliance and irritant receptor discharge in the anaesthetized dog.", "content": "1 The effects of histamine and 4 methylhistamine (i.v.) alone, and in the presence of chlorpheniramine or cimetidine, on total lung resistance (RL), dynamic lung compliance (Cdyn) and irritant receptor activity have been studied in dogs anaesthetized with chloralose. 2 Histamine produced dose-related increases in RL and irritant receptor activity with associated falls in Cdyn which were blocked by chlorpheniramine but unaffected by cimetidine. 3 4 Methylhistamine produced small insignificant changes in RL and Cdyn and small significant increases in irritant receptor activity which were reduced with chlorpheniramine but unaffected by cimetidine. 4 The results suggest that histamine increases irritant receptor activity, either directly or indirectly, via H1-receptors.", "contents": "The effects of H1- and H2-receptor agonists and antagonists on total lung resistance, dynamic lung compliance and irritant receptor discharge in the anaesthetized dog. 1 The effects of histamine and 4 methylhistamine (i.v.) alone, and in the presence of chlorpheniramine or cimetidine, on total lung resistance (RL), dynamic lung compliance (Cdyn) and irritant receptor activity have been studied in dogs anaesthetized with chloralose. 2 Histamine produced dose-related increases in RL and irritant receptor activity with associated falls in Cdyn which were blocked by chlorpheniramine but unaffected by cimetidine. 3 4 Methylhistamine produced small insignificant changes in RL and Cdyn and small significant increases in irritant receptor activity which were reduced with chlorpheniramine but unaffected by cimetidine. 4 The results suggest that histamine increases irritant receptor activity, either directly or indirectly, via H1-receptors."}
{"id": "PMID:465872", "title": "Effect of 5-hydroxytryptamine on protein synthesis in gastrointestinal and other tissues and on serum gastrin concentrations in rats.", "content": "1 The effect of 5-hydroxytryptamine (5-HT) on protein synthesis in the gastrointestinal tissues as well as in the liver, heart and brain was studied by measuring [3H]-leucine incorporation into total tissue protein in vivo in rats. 2 A single injection of 5-HT (10 mg/kg) produced a marked inhibition (45 to 65%) in protein synthesis in the stomach (oxyntic gland area), intestine (jejunum + ileum), colon and brain, but not in the liver and heart. 3 Dose- and time-dependent studies of 5-HT-induced changes in protein synthesis in the stomach of fed rats revealed that the maximal inhibition of about 65% occurred 1 h after a dose of 12.5 mg/kg. 4 Animals deprived of food for 24 h differed from their fed counterparts only in the degree of responsiveness, in that a greater inhibition (75%) of [3H]-leucine incorporation into total protein of the stomach was observed 30 min after 5-HT injection. 5 Pretreatment of animals with methysergide (0.25 mg/kg) essentially abolished the 5-HT-mediated inhibition of protein synthesis in the stomach. 6 Serum gastrin concentration in fasted animals remained slightly elevated during the initial period of 5-HT treatment (up to 1 h). 7 The results demonstrate that in certain tissues, 5-HT markedly inhibits protein synthesis. The inhibition in protein synthesis in the gastrointestinal tract cannot be attributed to changes in the concentration of gastrin.", "contents": "Effect of 5-hydroxytryptamine on protein synthesis in gastrointestinal and other tissues and on serum gastrin concentrations in rats. 1 The effect of 5-hydroxytryptamine (5-HT) on protein synthesis in the gastrointestinal tissues as well as in the liver, heart and brain was studied by measuring [3H]-leucine incorporation into total tissue protein in vivo in rats. 2 A single injection of 5-HT (10 mg/kg) produced a marked inhibition (45 to 65%) in protein synthesis in the stomach (oxyntic gland area), intestine (jejunum + ileum), colon and brain, but not in the liver and heart. 3 Dose- and time-dependent studies of 5-HT-induced changes in protein synthesis in the stomach of fed rats revealed that the maximal inhibition of about 65% occurred 1 h after a dose of 12.5 mg/kg. 4 Animals deprived of food for 24 h differed from their fed counterparts only in the degree of responsiveness, in that a greater inhibition (75%) of [3H]-leucine incorporation into total protein of the stomach was observed 30 min after 5-HT injection. 5 Pretreatment of animals with methysergide (0.25 mg/kg) essentially abolished the 5-HT-mediated inhibition of protein synthesis in the stomach. 6 Serum gastrin concentration in fasted animals remained slightly elevated during the initial period of 5-HT treatment (up to 1 h). 7 The results demonstrate that in certain tissues, 5-HT markedly inhibits protein synthesis. The inhibition in protein synthesis in the gastrointestinal tract cannot be attributed to changes in the concentration of gastrin."}
{"id": "PMID:465873", "title": "Evidence for an indirect cholinergic regulation of blood flow in the hypothalamus of conscious rabbits.", "content": "1 The effects of methacholine, atropine and adrenoceptor blockade on hypothalamic blood flow (HBF) were measured in conscious rabbits. 2 A dose of 1 microgram methacholine increased HBF while smaller and larger doses had no significant effect. 3 The vasodilatation induced by methacholine was blocked by atropine and by chemical sympathectomy of the hypothalamus with 6-hydroxydopamine. 4 The vasodilatation was reversed by propranolol but was not affected by phenoxybenzamine. 5 These results suggest that the vasodilator action of muscarinic receptor agonists on hypothalamic resistance vessels depends upon the integrity of a noradrenergic system, and is mediated via beta-adrenoceptors.", "contents": "Evidence for an indirect cholinergic regulation of blood flow in the hypothalamus of conscious rabbits. 1 The effects of methacholine, atropine and adrenoceptor blockade on hypothalamic blood flow (HBF) were measured in conscious rabbits. 2 A dose of 1 microgram methacholine increased HBF while smaller and larger doses had no significant effect. 3 The vasodilatation induced by methacholine was blocked by atropine and by chemical sympathectomy of the hypothalamus with 6-hydroxydopamine. 4 The vasodilatation was reversed by propranolol but was not affected by phenoxybenzamine. 5 These results suggest that the vasodilator action of muscarinic receptor agonists on hypothalamic resistance vessels depends upon the integrity of a noradrenergic system, and is mediated via beta-adrenoceptors."}
{"id": "PMID:465874", "title": "Relationship between accumulation, storage and overflow of noradrenaline in the rat salivary gland after chronic treatment with guanethidine.", "content": "1 The effect of guanethidine on the endogenous noradrenaline (NA) content, accumulation and overflow of [3H]-noradrenaline ([3H]-NA) in the rat salivary gland was examined at various times after drug administration. 2 Twenty-four h after a single injection of guanethidine (1 or 10 mg/kg s.c.), the respective values for the endogenous NA content and for the accumulation and overflow of [3H]-NA were approximately 55, 85, 30%, and 15, 55, 10% of the controls. 3 Although [3H]-NA accumulation had returned to control levels within 48 h after the dose of 10 mg/kg guanethidine, the overflow of [3H]-NA evoked by electrical stimulation or excess potassium (K+) remained depressed. 4 After the low or the high dose of guanethidine, the NA content of the salivary gland was restored to about 50% of the normal value between 4 to 24 and 48 to 72 h, respectively. 5 The accumulation of [3H]-NA was inhibited by about 75% by cocaine. The same degree of inhibition was obtained 4 h after 10 mg/kg guanethidine. In these experiments phenoxybenzamine did not reduce the residual (25%) uptake. 6 The reasons for differential rates of recovery of the endogenous NA content and the storage of [3H]-NA after guanethidine are discussed.", "contents": "Relationship between accumulation, storage and overflow of noradrenaline in the rat salivary gland after chronic treatment with guanethidine. 1 The effect of guanethidine on the endogenous noradrenaline (NA) content, accumulation and overflow of [3H]-noradrenaline ([3H]-NA) in the rat salivary gland was examined at various times after drug administration. 2 Twenty-four h after a single injection of guanethidine (1 or 10 mg/kg s.c.), the respective values for the endogenous NA content and for the accumulation and overflow of [3H]-NA were approximately 55, 85, 30%, and 15, 55, 10% of the controls. 3 Although [3H]-NA accumulation had returned to control levels within 48 h after the dose of 10 mg/kg guanethidine, the overflow of [3H]-NA evoked by electrical stimulation or excess potassium (K+) remained depressed. 4 After the low or the high dose of guanethidine, the NA content of the salivary gland was restored to about 50% of the normal value between 4 to 24 and 48 to 72 h, respectively. 5 The accumulation of [3H]-NA was inhibited by about 75% by cocaine. The same degree of inhibition was obtained 4 h after 10 mg/kg guanethidine. In these experiments phenoxybenzamine did not reduce the residual (25%) uptake. 6 The reasons for differential rates of recovery of the endogenous NA content and the storage of [3H]-NA after guanethidine are discussed."}
{"id": "PMID:465875", "title": "Effects of beta 2-adrenoceptor stimulation on cardiac metabolism in the conscious dog.", "content": "1 The effects of the beta 2-adrenoceptor stimulant, salbutamol, on cardiac metabolism have been studied in conscious mongrel dogs. The potential effects of anaesthesia on the study of cardiac metabolism have been avoided by prior implantation of arterial (A) and coronary sinus (CS) catheters for blood sampling and a central venous catheter for infusion. Extraction of substrates for myocardial energy metabolism (CA-CS) was assessed 3 to 24 days post-operatively. A 100 micrograms bolus of salbutamol was given followed by an infusion of 3 micrograms/min for 1 h. 2 Although heart rate increased significantly from 106 to 165 beats/min, fractional extraction of oxygen tended to fall from 84% to 77%. Thus an increase in coronary blood flow rather than in oxygen extraction must have maintained an oxygen supply commensurate with the salbutamol-induced tachycardia. 3 Neither CA-CS glucose nor fractional glucose extraction altered significantly during salbutamol infusion despite increases in arterial concentration (CA) of glucose and arterial insulin immunoreactivity and a decrease in CA of free fatty acids (FFA). This suggests that an insulin-antagonistic action accompanies the infusion of salbutamol. 4 The fractional extraction of lactate increased during salbutamol infusion. In part, this may have been a reflection of a decreased myocardial extraction of FFA with salbutamol in this model.", "contents": "Effects of beta 2-adrenoceptor stimulation on cardiac metabolism in the conscious dog. 1 The effects of the beta 2-adrenoceptor stimulant, salbutamol, on cardiac metabolism have been studied in conscious mongrel dogs. The potential effects of anaesthesia on the study of cardiac metabolism have been avoided by prior implantation of arterial (A) and coronary sinus (CS) catheters for blood sampling and a central venous catheter for infusion. Extraction of substrates for myocardial energy metabolism (CA-CS) was assessed 3 to 24 days post-operatively. A 100 micrograms bolus of salbutamol was given followed by an infusion of 3 micrograms/min for 1 h. 2 Although heart rate increased significantly from 106 to 165 beats/min, fractional extraction of oxygen tended to fall from 84% to 77%. Thus an increase in coronary blood flow rather than in oxygen extraction must have maintained an oxygen supply commensurate with the salbutamol-induced tachycardia. 3 Neither CA-CS glucose nor fractional glucose extraction altered significantly during salbutamol infusion despite increases in arterial concentration (CA) of glucose and arterial insulin immunoreactivity and a decrease in CA of free fatty acids (FFA). This suggests that an insulin-antagonistic action accompanies the infusion of salbutamol. 4 The fractional extraction of lactate increased during salbutamol infusion. In part, this may have been a reflection of a decreased myocardial extraction of FFA with salbutamol in this model."}
{"id": "PMID:465876", "title": "Cardiovascular responses produced by the injection of dopamine into the cerebral ventricles of the unanaesthetized dog.", "content": "1 The injection of dopamine (100 to 500 microgram) into the cerebral ventricles (i.c.v.) of 10 unanaesthetized dogs produced a dose-dependent increase in arterial blood pressure and heart rate. The dogs licked, swallowed, sometimes vomited and became sedated. 2 Autonomic ganglion blockade with hexamethonium (10 mg/kg i.v.) abolished cardiovascular responses to i.c.v. dopamine, indicating that dopamine was exerting its effect within the central nervous system. 3 The dopamine receptor antagonists, haloperidol (500 microgram), chlorpromazine (200 micrograms) and ergometrine (500 micrograms), each given i.c.v., sugsequently abolished the cardiovascular responses to dopamine. 4 Pretreatment with either the beta-adrenoceptor antagonist, propranolol (600 microgram) or the alpha-adrenoceptor antagonist, phentolamine (1 mg) given i.c.v. had no significant effect on the response to dopamine. 5 It is suggested that dopamine injected into the cerebral ventricles of the unanesthetized dog causes hypertension and tachycardia by activating central dopamine receptors.", "contents": "Cardiovascular responses produced by the injection of dopamine into the cerebral ventricles of the unanaesthetized dog. 1 The injection of dopamine (100 to 500 microgram) into the cerebral ventricles (i.c.v.) of 10 unanaesthetized dogs produced a dose-dependent increase in arterial blood pressure and heart rate. The dogs licked, swallowed, sometimes vomited and became sedated. 2 Autonomic ganglion blockade with hexamethonium (10 mg/kg i.v.) abolished cardiovascular responses to i.c.v. dopamine, indicating that dopamine was exerting its effect within the central nervous system. 3 The dopamine receptor antagonists, haloperidol (500 microgram), chlorpromazine (200 micrograms) and ergometrine (500 micrograms), each given i.c.v., sugsequently abolished the cardiovascular responses to dopamine. 4 Pretreatment with either the beta-adrenoceptor antagonist, propranolol (600 microgram) or the alpha-adrenoceptor antagonist, phentolamine (1 mg) given i.c.v. had no significant effect on the response to dopamine. 5 It is suggested that dopamine injected into the cerebral ventricles of the unanesthetized dog causes hypertension and tachycardia by activating central dopamine receptors."}
{"id": "PMID:465877", "title": "The effects of disopyramide phosphate on early post-coronary artery ligation dysrhythmias and on epicardial ST-segment elevation in anaesthetized dogs.", "content": "1 The antidysrhythmic, haemodynamic and metabolic effects of intravenously administered disopyramide phosphate (1 to 5 mg/kg) have been studied in greyhounds, anaesthetized with trichloroethylene. 2 In doses of 2.5 and 5.0 mg/kg, disopyramide significantly reduced the ventricular dysrhythmias that occur in the initial 30-min period following acute coronary artery ligation. None of the disopyramide-treated animals developed ventricular fibrillations. 3 The metabolic consequences of coronary artery ligation, assessed by local coronary venous sampling from the ischaemic area, were not modified by disopyramide except that K+ egress was prevented. 4 There was evidence for substantial disopyramide-induced myocardial depression (decreased cardiac output and left ventricular dP/drmax with elevated ventricular filling pressure and pulmonary oedema and shunting) and it is suggested that great care be taken when the drug is administered intravenously in conditions where cardiac function is already compromised. Disopyramide also reduced myocardial blood flow. 5 In chloralose-anaesthetized mongrel dogs, disopyramide (2.5 mg/kg) significantly reduced the ST-segment elevation (assessed from epicardial recordings) that resulted from short (3 min) coronary artery occlusions. This could indicate a reduction in the extent and severity of myocardial injury or simply reflect decreased K+ efflux (since locally administered K+ itself increased ST-segment elevation).", "contents": "The effects of disopyramide phosphate on early post-coronary artery ligation dysrhythmias and on epicardial ST-segment elevation in anaesthetized dogs. 1 The antidysrhythmic, haemodynamic and metabolic effects of intravenously administered disopyramide phosphate (1 to 5 mg/kg) have been studied in greyhounds, anaesthetized with trichloroethylene. 2 In doses of 2.5 and 5.0 mg/kg, disopyramide significantly reduced the ventricular dysrhythmias that occur in the initial 30-min period following acute coronary artery ligation. None of the disopyramide-treated animals developed ventricular fibrillations. 3 The metabolic consequences of coronary artery ligation, assessed by local coronary venous sampling from the ischaemic area, were not modified by disopyramide except that K+ egress was prevented. 4 There was evidence for substantial disopyramide-induced myocardial depression (decreased cardiac output and left ventricular dP/drmax with elevated ventricular filling pressure and pulmonary oedema and shunting) and it is suggested that great care be taken when the drug is administered intravenously in conditions where cardiac function is already compromised. Disopyramide also reduced myocardial blood flow. 5 In chloralose-anaesthetized mongrel dogs, disopyramide (2.5 mg/kg) significantly reduced the ST-segment elevation (assessed from epicardial recordings) that resulted from short (3 min) coronary artery occlusions. This could indicate a reduction in the extent and severity of myocardial injury or simply reflect decreased K+ efflux (since locally administered K+ itself increased ST-segment elevation)."}
{"id": "PMID:465878", "title": "An analysis of the negative inotropic action of somatostatin.", "content": "1 Somatostatin (SS) was evaluated as a chronotropic and inotropic agent in isolated spontaneously beating auricles of rats, rabbits and guinea-pigs.2 SS was completely inactive in rat and rabbit auricles but exerted a dose-dependent, negative inotropic effect in guinea-pig auricles in concentrations between 1.5 x 10(-8) to 1.2 x 10(-6) M.3 The negative inotropic effect of SS (6.0 x 10(-8) and 3.0 x 10(-7) M) was not inhibited by a mixture of antagonists containing practolol (7.9 x 10(-6) M), phentolamine (3.5 x 10(-7) M), methysergide (2.8 x 10(-7) M), diphenhydramine (3.9 x 10(-5) M), cimetidine (4.0 x 10(-5) M) atropine (3.4 x 10(-7) M) and indomethacin (1.4 x 10(-5) M).4 The negative inotropic effect of SS was greatly potentiated by reduction in the Ca(2+) concentration of the medium from 5.0 to 1.25 mM.5 On a molar basis, SS was equipotent with acetylcholine (ACh) as a negative inotropic agent in the guinea-pig auricles.6 SS (6.0 x 10(-8) and 6.0 x 10(-7) M) was found to inhibit selectively the positive inotropic action of neurotensin (NT) in guinea-pig but not in rat auricles.7 The inhibitory action of SS against NT was independent of its negative inotropic action.8 These results suggest that SS produces its negative inotropic action by interacting with specific receptors presumably located in the cell membrane of guinea-pig atria. The interaction between SS and its receptor may cause a decreased Ca(2+) diffusion and/or transport into the atrial cells. The physiological and pharmacological significance of these results is discussed.", "contents": "An analysis of the negative inotropic action of somatostatin. 1 Somatostatin (SS) was evaluated as a chronotropic and inotropic agent in isolated spontaneously beating auricles of rats, rabbits and guinea-pigs.2 SS was completely inactive in rat and rabbit auricles but exerted a dose-dependent, negative inotropic effect in guinea-pig auricles in concentrations between 1.5 x 10(-8) to 1.2 x 10(-6) M.3 The negative inotropic effect of SS (6.0 x 10(-8) and 3.0 x 10(-7) M) was not inhibited by a mixture of antagonists containing practolol (7.9 x 10(-6) M), phentolamine (3.5 x 10(-7) M), methysergide (2.8 x 10(-7) M), diphenhydramine (3.9 x 10(-5) M), cimetidine (4.0 x 10(-5) M) atropine (3.4 x 10(-7) M) and indomethacin (1.4 x 10(-5) M).4 The negative inotropic effect of SS was greatly potentiated by reduction in the Ca(2+) concentration of the medium from 5.0 to 1.25 mM.5 On a molar basis, SS was equipotent with acetylcholine (ACh) as a negative inotropic agent in the guinea-pig auricles.6 SS (6.0 x 10(-8) and 6.0 x 10(-7) M) was found to inhibit selectively the positive inotropic action of neurotensin (NT) in guinea-pig but not in rat auricles.7 The inhibitory action of SS against NT was independent of its negative inotropic action.8 These results suggest that SS produces its negative inotropic action by interacting with specific receptors presumably located in the cell membrane of guinea-pig atria. The interaction between SS and its receptor may cause a decreased Ca(2+) diffusion and/or transport into the atrial cells. The physiological and pharmacological significance of these results is discussed."}
{"id": "PMID:465879", "title": "The effect of some general anaesthetics on the surface potential of lipid monolayers.", "content": "1 This study sought to investigate the report by Ginsberg (1978) that 0.7 M ethanol brought about a + 100 mV change (DeltaDeltaV) in the surface potential of glyceryl monooleate (GMO) monolayers formed on KCl, although he predicted that a DeltaDeltaV of -10 mV should have been found.2 The effect of general anaesthetics such as n-alkyl alcohols and pentobarbitone on surface potential (DeltaV) and surface tension (gamma) of lipid monolayers formed on 145 mM KCl from either glyceryl monooleate (GMO) or phosphatidyl choline (PC) was examined with an Americium-241 air electrode assembly (DeltaV) and a platinized platinum dipping plate and force balance (gamma).3 It was found that, as predicted by Ginsberg (1978), addition of 0.7 M ethanol to the aqueous phase bathing either PC or GMO monolayers brings about a negative-going change in interfacial potential (DeltaDeltaV).4 The magnitude of DeltaDeltaV is dependent in a linear fashion on ethanol concentration.5 Longer chain length alcohols up to n-decanol also bring about a negative going change in DeltaDeltaV, and the dependence of DeltaDeltaV on anaesthetic activity, with respect to increasing chain length of anaesthetic, is consistent with Traube's law.6 Pentobarbitone added to the aqueous phase bathing the monolayer also elicits a negative DeltaDeltaV, a finding which rules out the possibility of adsorption of the volatile alcohols to the measuring electrode.7 The findings are discussed in terms of the proposition that increasing disorder in an array of fixed dipoles, such as might occur in a bilayer exposed to anaesthetic, would result in a lowering of the electrostatic barrier to the predominantly impermeable cation.", "contents": "The effect of some general anaesthetics on the surface potential of lipid monolayers. 1 This study sought to investigate the report by Ginsberg (1978) that 0.7 M ethanol brought about a + 100 mV change (DeltaDeltaV) in the surface potential of glyceryl monooleate (GMO) monolayers formed on KCl, although he predicted that a DeltaDeltaV of -10 mV should have been found.2 The effect of general anaesthetics such as n-alkyl alcohols and pentobarbitone on surface potential (DeltaV) and surface tension (gamma) of lipid monolayers formed on 145 mM KCl from either glyceryl monooleate (GMO) or phosphatidyl choline (PC) was examined with an Americium-241 air electrode assembly (DeltaV) and a platinized platinum dipping plate and force balance (gamma).3 It was found that, as predicted by Ginsberg (1978), addition of 0.7 M ethanol to the aqueous phase bathing either PC or GMO monolayers brings about a negative-going change in interfacial potential (DeltaDeltaV).4 The magnitude of DeltaDeltaV is dependent in a linear fashion on ethanol concentration.5 Longer chain length alcohols up to n-decanol also bring about a negative going change in DeltaDeltaV, and the dependence of DeltaDeltaV on anaesthetic activity, with respect to increasing chain length of anaesthetic, is consistent with Traube's law.6 Pentobarbitone added to the aqueous phase bathing the monolayer also elicits a negative DeltaDeltaV, a finding which rules out the possibility of adsorption of the volatile alcohols to the measuring electrode.7 The findings are discussed in terms of the proposition that increasing disorder in an array of fixed dipoles, such as might occur in a bilayer exposed to anaesthetic, would result in a lowering of the electrostatic barrier to the predominantly impermeable cation."}
{"id": "PMID:465880", "title": "Responses to DL-ibotenic acid at locust glutamatergic neuromuscular junctions.", "content": "1 The responses of excitatory junctions on locust skeletal muscle fibres to iontophoretically applied L-glutamic acid and DL-ibotenic acid, a rigidly extended analogue of glutamate, were recorded by means of intracellular microelectrodes.2 Iontophoresis of L-glutamate to junctional sites produced transient depolarizations. Ibotenate applied iontophoretically to these sites usually evoked small hyperpolarizations which probably resulted from the activation of glutamate H-receptors on the extrajunctional membrane surrounding the junctions. However, at a minority ( approximately 20%) of junctions, ibotenate iontophoresis evoked transient depolarizations.3 Iontophoretically applied glutamate desensitized the ibotenate receptors, and vice versa. In experiments performed at junctional sites at which ibotenate depolarizations were absent, ibotenate had no effect on the responses to glutamate.4 Glutamate and ibotenate junctional currents had similar reversal potentials, measured under voltage-clamp, suggesting that the ionic bases for these currents are identical.5 It is proposed that the excitation caused by ibotenate results from the activation of receptors for extended L-glutamate and that these receptors co-exist on the post-junctional membranes of locust excitatory nerve-muscle synapses with ibotenate-insensitive glutamate receptors activated by glutamate in partially folded conformation.", "contents": "Responses to DL-ibotenic acid at locust glutamatergic neuromuscular junctions. 1 The responses of excitatory junctions on locust skeletal muscle fibres to iontophoretically applied L-glutamic acid and DL-ibotenic acid, a rigidly extended analogue of glutamate, were recorded by means of intracellular microelectrodes.2 Iontophoresis of L-glutamate to junctional sites produced transient depolarizations. Ibotenate applied iontophoretically to these sites usually evoked small hyperpolarizations which probably resulted from the activation of glutamate H-receptors on the extrajunctional membrane surrounding the junctions. However, at a minority ( approximately 20%) of junctions, ibotenate iontophoresis evoked transient depolarizations.3 Iontophoretically applied glutamate desensitized the ibotenate receptors, and vice versa. In experiments performed at junctional sites at which ibotenate depolarizations were absent, ibotenate had no effect on the responses to glutamate.4 Glutamate and ibotenate junctional currents had similar reversal potentials, measured under voltage-clamp, suggesting that the ionic bases for these currents are identical.5 It is proposed that the excitation caused by ibotenate results from the activation of receptors for extended L-glutamate and that these receptors co-exist on the post-junctional membranes of locust excitatory nerve-muscle synapses with ibotenate-insensitive glutamate receptors activated by glutamate in partially folded conformation."}
{"id": "PMID:465881", "title": "Decreased binding of drugs and dyes to plasma proteins from rats with acute renal failure: effects of ureter ligation and intramuscular injection of glycerol.", "content": "1 The decreased binding of drugs and dyes to plasma proteins from male and female rats with acute renal failure has been investigated using equilibrium dialysis at 37 degrees C. 2 Acute renal failure induced by bilateral ligation of the ureters produced a greater than threefold increase in the unbound fraction of o-methyl red relative to normal rat plasma. Unbound dye concentration in plasma from sham-operated control rats was also significantly increased but to a lesser extent. 3 Glycerol-induced acute renal failure produced a significant increase in the unbound fractions of o-methyl red, methyl orange, bromocresol green (BCG), 2-(4'-hydroxybenzeneazo) benzoic acid (HABA), phenytoin and salicylic acid. A marginally significant increase in unbound warfarin concentration was observed. 4 Glycerol-induced renal failure had no effect on total plasma protein concentration and experiments with o-methyl red and salicylic acid indicated that a direct effect of glycerol was not responsible for the diminution of binding. 5 Glycerol-induced acute renal failure, which produced decreases in drug and dye binding similar to those reported for human uraemic plasma, provides a convenient non-surgical animal model for the investigation of this phenomenon.", "contents": "Decreased binding of drugs and dyes to plasma proteins from rats with acute renal failure: effects of ureter ligation and intramuscular injection of glycerol. 1 The decreased binding of drugs and dyes to plasma proteins from male and female rats with acute renal failure has been investigated using equilibrium dialysis at 37 degrees C. 2 Acute renal failure induced by bilateral ligation of the ureters produced a greater than threefold increase in the unbound fraction of o-methyl red relative to normal rat plasma. Unbound dye concentration in plasma from sham-operated control rats was also significantly increased but to a lesser extent. 3 Glycerol-induced acute renal failure produced a significant increase in the unbound fractions of o-methyl red, methyl orange, bromocresol green (BCG), 2-(4'-hydroxybenzeneazo) benzoic acid (HABA), phenytoin and salicylic acid. A marginally significant increase in unbound warfarin concentration was observed. 4 Glycerol-induced renal failure had no effect on total plasma protein concentration and experiments with o-methyl red and salicylic acid indicated that a direct effect of glycerol was not responsible for the diminution of binding. 5 Glycerol-induced acute renal failure, which produced decreases in drug and dye binding similar to those reported for human uraemic plasma, provides a convenient non-surgical animal model for the investigation of this phenomenon."}
{"id": "PMID:465882", "title": "Rapid absorption from the urinary bladder of a series of n-alkyl carbamates: a route for the recirculation of drugs.", "content": "1 The rate of loss of a series of n-alkyl carbamates from the lumen of the urinary bladders of female rats has been studied. 2 The rate of loss obeys first order kinetics and was not affected by water flux across the bladder wall nor by binding of the carbamates to it. 3 The rate of loss of octyl carbamate was reduced by about 76% by the presence of 5% Tween 80. Histological evidence indicates that this may be due to the formation of a thin luminal lining which may be adsorbed Tween 80 or mucopolysaccharide material. 4 The absorption rate of the carbamates was limited by their hydrophilicity but reached a plateau for the more lipophilic homologues with a half life for loss of approximately 10 min. 5 The implications of these results with regard to the recirculation of unmetabolized drugs and hydrolysed conjugates of drugs, the systemic absorption of intravesically applied cancer chemotherapeutic agents and bladder wall permeability to carcinogens are discussed.", "contents": "Rapid absorption from the urinary bladder of a series of n-alkyl carbamates: a route for the recirculation of drugs. 1 The rate of loss of a series of n-alkyl carbamates from the lumen of the urinary bladders of female rats has been studied. 2 The rate of loss obeys first order kinetics and was not affected by water flux across the bladder wall nor by binding of the carbamates to it. 3 The rate of loss of octyl carbamate was reduced by about 76% by the presence of 5% Tween 80. Histological evidence indicates that this may be due to the formation of a thin luminal lining which may be adsorbed Tween 80 or mucopolysaccharide material. 4 The absorption rate of the carbamates was limited by their hydrophilicity but reached a plateau for the more lipophilic homologues with a half life for loss of approximately 10 min. 5 The implications of these results with regard to the recirculation of unmetabolized drugs and hydrolysed conjugates of drugs, the systemic absorption of intravesically applied cancer chemotherapeutic agents and bladder wall permeability to carcinogens are discussed."}
{"id": "PMID:465883", "title": "Glutamate as the neurotransmitter of cerebellar granule cells in the rat: electrophysiological evidence.", "content": "1 Glutamate and the excitatory aminoacid antagonist, alpha-aminoadipic acid (alphaAA), have been applied by microiontophoresis to Purkinje cells in the rat cerebellum. 2 Glutemate produced excitation of Purkinje cells and alpha AA selectively reduced that excitation without affecting responses to acetylcholine or hydrogen ions. 3 Monosynaptic spikes were evoked in Purkinje cells by stimulating the parallel fibres. alpha AA had little effect on these spikes when applied alone. 4 When the Purkinje cell excitability was reduced by the iontophoresis of gamma-aminobutyric acid, alpha AA then produced railure of the monosynaptic spike on 10 of 13 Purkinje cells, in doses shown to be selectively antagonistic towards aminoacids. 5 These results support neurochemical evidence that glutamic acid may be the neurotransmitter released by granule cell parallel fibres.", "contents": "Glutamate as the neurotransmitter of cerebellar granule cells in the rat: electrophysiological evidence. 1 Glutamate and the excitatory aminoacid antagonist, alpha-aminoadipic acid (alphaAA), have been applied by microiontophoresis to Purkinje cells in the rat cerebellum. 2 Glutemate produced excitation of Purkinje cells and alpha AA selectively reduced that excitation without affecting responses to acetylcholine or hydrogen ions. 3 Monosynaptic spikes were evoked in Purkinje cells by stimulating the parallel fibres. alpha AA had little effect on these spikes when applied alone. 4 When the Purkinje cell excitability was reduced by the iontophoresis of gamma-aminobutyric acid, alpha AA then produced railure of the monosynaptic spike on 10 of 13 Purkinje cells, in doses shown to be selectively antagonistic towards aminoacids. 5 These results support neurochemical evidence that glutamic acid may be the neurotransmitter released by granule cell parallel fibres."}
{"id": "PMID:465884", "title": "On the distribution of [met5]- and [leu5]-enkephalins in the brain of the rat, guinea-pig and calf.", "content": "1 By means of a highly sensitive radioimmunoassay, the content of [met5]-enkephalin (ME) and [leu5]-enkephalin (LE) was measured in various regions of the rat, guinea-pig and calf brain. Provisions were made to differentiate ME from LE by the use of cyanogen bromide (CNBr) to destroy methionine, the carboxy terminal amino acid in the ME sequence. This allows for correction of possible errors due to the cross-reactivity of the ME to the LE antiserum. Evidence is presented to demonstrate that the specific radioimmunoassay combined with the CNBr technique is a valid method to measure LE and ME content in crude tissue extracts. 2 In all the species studied, enkephalins appeared to be highly concentrated in the striatum and hypothalamus while very low amounts were found in the cerebe-lum and hippocampus. 3 Although the ratio between ME and LE content varied from area to area, the ME content in every region of the rat, guinea-pig and calf brain was more than 4 fold greater than that of LE.", "contents": "On the distribution of [met5]- and [leu5]-enkephalins in the brain of the rat, guinea-pig and calf. 1 By means of a highly sensitive radioimmunoassay, the content of [met5]-enkephalin (ME) and [leu5]-enkephalin (LE) was measured in various regions of the rat, guinea-pig and calf brain. Provisions were made to differentiate ME from LE by the use of cyanogen bromide (CNBr) to destroy methionine, the carboxy terminal amino acid in the ME sequence. This allows for correction of possible errors due to the cross-reactivity of the ME to the LE antiserum. Evidence is presented to demonstrate that the specific radioimmunoassay combined with the CNBr technique is a valid method to measure LE and ME content in crude tissue extracts. 2 In all the species studied, enkephalins appeared to be highly concentrated in the striatum and hypothalamus while very low amounts were found in the cerebe-lum and hippocampus. 3 Although the ratio between ME and LE content varied from area to area, the ME content in every region of the rat, guinea-pig and calf brain was more than 4 fold greater than that of LE."}
{"id": "PMID:465885", "title": "Effects of noradrenaline and carbachol on temperature regulation of cold-stressed and cold-acclimated rats.", "content": "1 Noradrenaline (20 micrograms) and carbachol (1 microgram) injected into the anterior hypothalamus of rats at an ambient temperature of 23 degrees C evoked significant falls in core temperature and increases in tail temperature. 2 When rats were cold-stressed (4 degrees C for 90 min) or cold-acclimated (4 degrees C for 4 weeks) and the above amine injections repeated, only carbachol evoked significant falls in core temperature and neither amine increased tail temperature. 3 Central injections of noradrenaline and carbachol also evoked increases in plasma glucose concentrations but not plasma non-esterified fatty acid (NEFA) concentrations in control, acutely cold-stressed and cold-acclimated rats. 4 Although concentrations of plasma glucose and blood lactate of rats were unaffected by cold exposure to 4 degrees C for 1 to 28 days, glucose oxidation rate of both cold-stressed and cold-acclimated rats was significantly greater than in rats at 23 degrees C. Concentrations of plasma NEFA were increased after 1 to 28 days of cold exposure.", "contents": "Effects of noradrenaline and carbachol on temperature regulation of cold-stressed and cold-acclimated rats. 1 Noradrenaline (20 micrograms) and carbachol (1 microgram) injected into the anterior hypothalamus of rats at an ambient temperature of 23 degrees C evoked significant falls in core temperature and increases in tail temperature. 2 When rats were cold-stressed (4 degrees C for 90 min) or cold-acclimated (4 degrees C for 4 weeks) and the above amine injections repeated, only carbachol evoked significant falls in core temperature and neither amine increased tail temperature. 3 Central injections of noradrenaline and carbachol also evoked increases in plasma glucose concentrations but not plasma non-esterified fatty acid (NEFA) concentrations in control, acutely cold-stressed and cold-acclimated rats. 4 Although concentrations of plasma glucose and blood lactate of rats were unaffected by cold exposure to 4 degrees C for 1 to 28 days, glucose oxidation rate of both cold-stressed and cold-acclimated rats was significantly greater than in rats at 23 degrees C. Concentrations of plasma NEFA were increased after 1 to 28 days of cold exposure."}
{"id": "PMID:465886", "title": "Improved insulin receptor assay: effects of an antidiabetic sulphonylurea on liver membrane insulin receptors from obese hyperglycaemic mice.", "content": "1 A simple assay of liver membrane insulin receptors is described, under conditions which apparently stabilized the insulin-receptor complex. The receptor population was measured in the presence of physiological doses of radioactive hormone under conditions which minimized negative co-operativity effects. 2 Equilibrium between receptor and hormone was attained in vitro within 60 min of incubation time at 13 degrees C with no apparent loss of binding sites after 3 h of incubation. 3 Liver membranes from lean mice possessed 8.8 times the receptor number per mg membrane protein measured in membranes from obese hyperglycaemic (ob/ob) littermates. After treatment of ob/ob mice with ARDF-26 (Gliquidon; Boehringer Ingelheim Ltd) the concentration of liver membrane insulin receptors was increased 5 fold.", "contents": "Improved insulin receptor assay: effects of an antidiabetic sulphonylurea on liver membrane insulin receptors from obese hyperglycaemic mice. 1 A simple assay of liver membrane insulin receptors is described, under conditions which apparently stabilized the insulin-receptor complex. The receptor population was measured in the presence of physiological doses of radioactive hormone under conditions which minimized negative co-operativity effects. 2 Equilibrium between receptor and hormone was attained in vitro within 60 min of incubation time at 13 degrees C with no apparent loss of binding sites after 3 h of incubation. 3 Liver membranes from lean mice possessed 8.8 times the receptor number per mg membrane protein measured in membranes from obese hyperglycaemic (ob/ob) littermates. After treatment of ob/ob mice with ARDF-26 (Gliquidon; Boehringer Ingelheim Ltd) the concentration of liver membrane insulin receptors was increased 5 fold."}
{"id": "PMID:465887", "title": "Relationship between inhibition of acetylcholinesterase and response of the rat phrenic nerve-diaphragm preparation to indirect stimulation at higher frequencies.", "content": "1 Rat isolated diaphragm preparations were stimulated indirectly either intermittently at 20, 50 or 100 Hz or continuously at 0.2 Hz.2 Addition of 1.8 muM paraoxon (which inhibits acetylcholinesterase by forming a phosphorylated enzyme which undergoes slow spontaneous reactivation) for 5 min to the organ bath produced a failure of the muscle to maintain tetanic tension (tetanic fade, Wedensky inhibition) and potentiated the neuromuscular blocking activity of exogenous acetylcholine. The rates of recovery from both these effects were recorded.3 In a series of experiments with dyflos (which inhibits acetylcholinesterase by forming a phosphorylated enzyme which does not undergo spontaneous reactivation) the relationship between functional acetylcholinesterase activity and neuromuscular blocking activity of exogenous acetylcholine was also determined.4 From the data obtained, the relationship between functional acetylcholinesterase activity and tetanic fade was calculated. These calculations show that (i) a considerable reduction in functional acetylcholinesterase activity is required before the diaphragm loses its ability to respond with a sustained tetanus to indirect stimulation at higher frequencies, (ii) the minimum (critical) level of functional acetylcholinesterase activity required for a normal tetanic response is directly related to the frequency of stimulation and (iii) once functional acetylcholinesterase activity has been reduced to the critical level, a very small further reduction leads to a complete tetanic fade.5 The meaning of functional acetylcholinesterase assays and of conclusions which can be drawn from them, is discussed.", "contents": "Relationship between inhibition of acetylcholinesterase and response of the rat phrenic nerve-diaphragm preparation to indirect stimulation at higher frequencies. 1 Rat isolated diaphragm preparations were stimulated indirectly either intermittently at 20, 50 or 100 Hz or continuously at 0.2 Hz.2 Addition of 1.8 muM paraoxon (which inhibits acetylcholinesterase by forming a phosphorylated enzyme which undergoes slow spontaneous reactivation) for 5 min to the organ bath produced a failure of the muscle to maintain tetanic tension (tetanic fade, Wedensky inhibition) and potentiated the neuromuscular blocking activity of exogenous acetylcholine. The rates of recovery from both these effects were recorded.3 In a series of experiments with dyflos (which inhibits acetylcholinesterase by forming a phosphorylated enzyme which does not undergo spontaneous reactivation) the relationship between functional acetylcholinesterase activity and neuromuscular blocking activity of exogenous acetylcholine was also determined.4 From the data obtained, the relationship between functional acetylcholinesterase activity and tetanic fade was calculated. These calculations show that (i) a considerable reduction in functional acetylcholinesterase activity is required before the diaphragm loses its ability to respond with a sustained tetanus to indirect stimulation at higher frequencies, (ii) the minimum (critical) level of functional acetylcholinesterase activity required for a normal tetanic response is directly related to the frequency of stimulation and (iii) once functional acetylcholinesterase activity has been reduced to the critical level, a very small further reduction leads to a complete tetanic fade.5 The meaning of functional acetylcholinesterase assays and of conclusions which can be drawn from them, is discussed."}
{"id": "PMID:465888", "title": "Relaxant response of goat trachea to 5-hydroxy-tryptamine mediated by D-tryptamine receptors.", "content": "1 Goat isolated trachea contracted in response to carbachol, histamine and 2-pyridylethylamine (an H(1)-receptor agonist) and relaxed after application of isoprenaline. 5-hydroxytryptamine (5-HT) and phenylephrine.2 Mepyramine, a selective H(1)-receptor antagonist, blocked histamine- and 2-pyridylethylamine-induced contractions. In high doses it also exhibited some nonspecific antagonism to carbachol. After H(1)-receptor blockade, 4-methylhistamine and dimaprit (specific H(2)-agonists) relaxed the carbachol-contracted trachea.3 Propranolol, a beta-adrenoceptor blocker, antagonized relaxation in response to isoprenaline and phenylephrine. In high doses, it produced a reversal of the phenylephrine response.4 Indomethacin enhanced contractions in response to carbachol and histamine.5 Relaxation to 5-HT was not affected by propranolol, indomethacin, metiamide or cimetidine (H(2)-blockers). These findings appear to exclude the involvement of adrenergic, prostaglandinergic and H(2)-histaminergic mechanisms in the mediation of this response.6 Atropine potentiated 5-HT-induced relaxations. This suggests the participation of a ;masked' excitatory cholinergic mechanism.7 Methysergide, dibenamine and dibenzyline selectively antagonized or reversed 5-HT-induced relaxation. Dibenamine and dibenzyline enhanced relaxations to isoprenaline.8 This investigation showed (i) a relaxant response of goat trachea to 5-HT, mediated via D-muscular tryptamine receptors; (ii) a small population of excitatory M-neuronal tryptamine and alpha-adrenoceptors; and (iii) predominance of H(1)-histamine receptors in the goat trachea.", "contents": "Relaxant response of goat trachea to 5-hydroxy-tryptamine mediated by D-tryptamine receptors. 1 Goat isolated trachea contracted in response to carbachol, histamine and 2-pyridylethylamine (an H(1)-receptor agonist) and relaxed after application of isoprenaline. 5-hydroxytryptamine (5-HT) and phenylephrine.2 Mepyramine, a selective H(1)-receptor antagonist, blocked histamine- and 2-pyridylethylamine-induced contractions. In high doses it also exhibited some nonspecific antagonism to carbachol. After H(1)-receptor blockade, 4-methylhistamine and dimaprit (specific H(2)-agonists) relaxed the carbachol-contracted trachea.3 Propranolol, a beta-adrenoceptor blocker, antagonized relaxation in response to isoprenaline and phenylephrine. In high doses, it produced a reversal of the phenylephrine response.4 Indomethacin enhanced contractions in response to carbachol and histamine.5 Relaxation to 5-HT was not affected by propranolol, indomethacin, metiamide or cimetidine (H(2)-blockers). These findings appear to exclude the involvement of adrenergic, prostaglandinergic and H(2)-histaminergic mechanisms in the mediation of this response.6 Atropine potentiated 5-HT-induced relaxations. This suggests the participation of a ;masked' excitatory cholinergic mechanism.7 Methysergide, dibenamine and dibenzyline selectively antagonized or reversed 5-HT-induced relaxation. Dibenamine and dibenzyline enhanced relaxations to isoprenaline.8 This investigation showed (i) a relaxant response of goat trachea to 5-HT, mediated via D-muscular tryptamine receptors; (ii) a small population of excitatory M-neuronal tryptamine and alpha-adrenoceptors; and (iii) predominance of H(1)-histamine receptors in the goat trachea."}
{"id": "PMID:465889", "title": "A study of the muscarinic receptor by gel electrophoresis.", "content": "1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.", "contents": "A study of the muscarinic receptor by gel electrophoresis. 1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000."}
{"id": "PMID:465890", "title": "Single pulse stimulation of guinea-pig vas deferens and the presynaptic receptor hypothesis.", "content": "1 The effect of phenoxybenzamine on the efflux of [3H]-noradrenaline and the mechanical response to single pulse excitation of superfused guinea-pig vas deferens was determined to examine the validity of the currently accepted hypothesis of a presynaptic negative feedback system on adrenergic nerve terminals. 2 The adrenoceptor antagonist enhanced both the outflow of tritium and the mechanical response to single pulse stimulation. The efflux of labelled material and the responses to 4 pulses were also enhanced, as expected. 3 Blockade of neuronal and extraneuronal uptake did not by itself increase nerve-induced outflow or the mechanical response nor dated within the framework of a hypothesis that proposes that the enhancement of response and tritium efflux by phenoxybenzamine results from blockade of a feedback system whereby noradrenaline released by previous impulses inhibits its own subsequent release.", "contents": "Single pulse stimulation of guinea-pig vas deferens and the presynaptic receptor hypothesis. 1 The effect of phenoxybenzamine on the efflux of [3H]-noradrenaline and the mechanical response to single pulse excitation of superfused guinea-pig vas deferens was determined to examine the validity of the currently accepted hypothesis of a presynaptic negative feedback system on adrenergic nerve terminals. 2 The adrenoceptor antagonist enhanced both the outflow of tritium and the mechanical response to single pulse stimulation. The efflux of labelled material and the responses to 4 pulses were also enhanced, as expected. 3 Blockade of neuronal and extraneuronal uptake did not by itself increase nerve-induced outflow or the mechanical response nor dated within the framework of a hypothesis that proposes that the enhancement of response and tritium efflux by phenoxybenzamine results from blockade of a feedback system whereby noradrenaline released by previous impulses inhibits its own subsequent release."}
{"id": "PMID:465891", "title": "Noradrenaline release in rats during prolonged cold-stress and repeated swim-stress.", "content": "1 Plasma noradrenaline concentration in rats was measured during prolonged cold-stress and repeated swim-stress. 2 Cold exposure for 6 h caused a rise in plasma noradrenaline which reached a peak at 4 h. 3 Administration of desmethylimipramine and normetanephrine to block neuronal and extra-neuronal uptake of noradrenaline raised plasma noradrenaline concentration without changing the pattern of the response to cold exposure. 4 Repeated cold exposure on subsequent days produced no change in the pattern of plasma noradrenaline concentration. 5 Five successive 1-min swims at 30-min intervals caused a rise in plasma noradrenaline concentration which was maximal after the third swim. 6 It is suggested that prolonged and repeated activation of sympathetic nerve terminals leads to a decline in noradrenaline release.", "contents": "Noradrenaline release in rats during prolonged cold-stress and repeated swim-stress. 1 Plasma noradrenaline concentration in rats was measured during prolonged cold-stress and repeated swim-stress. 2 Cold exposure for 6 h caused a rise in plasma noradrenaline which reached a peak at 4 h. 3 Administration of desmethylimipramine and normetanephrine to block neuronal and extra-neuronal uptake of noradrenaline raised plasma noradrenaline concentration without changing the pattern of the response to cold exposure. 4 Repeated cold exposure on subsequent days produced no change in the pattern of plasma noradrenaline concentration. 5 Five successive 1-min swims at 30-min intervals caused a rise in plasma noradrenaline concentration which was maximal after the third swim. 6 It is suggested that prolonged and repeated activation of sympathetic nerve terminals leads to a decline in noradrenaline release."}
{"id": "PMID:465892", "title": "Effect of guanidine on release of noradrenaline from the perfused spleen of the cat.", "content": "1 Guanidine increased noradrenaline (NA) output at 5 Hz by 3 to 6 fold, and doubled it at 30 Hz. Onset of maximum activity was slow, and reversal was also slow. Output of NA induced by potassium, sodium deprivation, or tyramine was not affected. 2 NA output was doubled at low concentrations (1 to 2 mM) of guanidine, but maximal effect was obtained at 4 mM. At 10 mM, spontaneous release was occasionally observed. 3 The effect of guanidine on NA release was related to the external calcium concentration. Outputs which previously have been shown to be insignificant at 5 Hz in 0.25 and 0.75 mM calcium-Krebs solution were markedly enhanced by guanidine. Guanidine enhanced release at all calcium concentrations up to 7.5 mM, but maximum output was obtained at 2.5 mM. 4 Guanidine had no effect on the recovery of intra-arterially infused NA. 5 The effects of guanidine and tetraethyl-ammonium (TEA) on NA release at 5 Hz were additive. 6 Guanidine reversed the inhibition of NA release by guanethidine during nerve stimulation at 5 and 10 Hz, and the NA output increased nearly 2.5 fold after repeated stimulation of the nerves. Guanidine was less effective in reversing the inhibitory effects of guanethidine on NA release at 30 Hz. 7 Guanidine did not affect release of catecholamines (CA) from the perfused cat adrenal gland by splanchnic nerve stimulation. 8 It is suggested that guanidine enhances NA release partly by increasing the influx of calcium into the neurone during an action potential, and also by interfering with intracellular binding of calcium.", "contents": "Effect of guanidine on release of noradrenaline from the perfused spleen of the cat. 1 Guanidine increased noradrenaline (NA) output at 5 Hz by 3 to 6 fold, and doubled it at 30 Hz. Onset of maximum activity was slow, and reversal was also slow. Output of NA induced by potassium, sodium deprivation, or tyramine was not affected. 2 NA output was doubled at low concentrations (1 to 2 mM) of guanidine, but maximal effect was obtained at 4 mM. At 10 mM, spontaneous release was occasionally observed. 3 The effect of guanidine on NA release was related to the external calcium concentration. Outputs which previously have been shown to be insignificant at 5 Hz in 0.25 and 0.75 mM calcium-Krebs solution were markedly enhanced by guanidine. Guanidine enhanced release at all calcium concentrations up to 7.5 mM, but maximum output was obtained at 2.5 mM. 4 Guanidine had no effect on the recovery of intra-arterially infused NA. 5 The effects of guanidine and tetraethyl-ammonium (TEA) on NA release at 5 Hz were additive. 6 Guanidine reversed the inhibition of NA release by guanethidine during nerve stimulation at 5 and 10 Hz, and the NA output increased nearly 2.5 fold after repeated stimulation of the nerves. Guanidine was less effective in reversing the inhibitory effects of guanethidine on NA release at 30 Hz. 7 Guanidine did not affect release of catecholamines (CA) from the perfused cat adrenal gland by splanchnic nerve stimulation. 8 It is suggested that guanidine enhances NA release partly by increasing the influx of calcium into the neurone during an action potential, and also by interfering with intracellular binding of calcium."}
{"id": "PMID:465893", "title": "The release of prostaglandin E2 from the skin of the plaice, Pleuronectes platessa L.", "content": "1 A fungal extract which produces a cutaneous hypersensitivity reaction in the plaice, Pleuronectes platessa L., was incubated in vitro with the skin of this teleost fish. Samples of incubation media were assayed for smooth muscle stimulating activity. 2 Prostaglandin E2 was identified by bioassay, thin-layer chromatography, ultraviolet absorption spectroscopy and gas chromatography--mass spectrometry. Release from challenged skin was maximum after 60 min incubation. 3 Analysis of the fatty acid composition of plaice skin showed that although arachidonic acid was present (3% of total fatty acids), the precursor of prostaglandin E3, eicosapentaenoic acid contributed 9% of total. 4 Indomethacin (50 mg/kg i.p) did not inhibit the erythema induced by the fungal extract, whilst a dose of 1 mg/kg maximally inhibited prostaglandin release from skin on incubation in vitro. 5 It is concluded that prostaglandins do not have an exclusive role in the mediation of the hypersensitivity reaction.", "contents": "The release of prostaglandin E2 from the skin of the plaice, Pleuronectes platessa L. 1 A fungal extract which produces a cutaneous hypersensitivity reaction in the plaice, Pleuronectes platessa L., was incubated in vitro with the skin of this teleost fish. Samples of incubation media were assayed for smooth muscle stimulating activity. 2 Prostaglandin E2 was identified by bioassay, thin-layer chromatography, ultraviolet absorption spectroscopy and gas chromatography--mass spectrometry. Release from challenged skin was maximum after 60 min incubation. 3 Analysis of the fatty acid composition of plaice skin showed that although arachidonic acid was present (3% of total fatty acids), the precursor of prostaglandin E3, eicosapentaenoic acid contributed 9% of total. 4 Indomethacin (50 mg/kg i.p) did not inhibit the erythema induced by the fungal extract, whilst a dose of 1 mg/kg maximally inhibited prostaglandin release from skin on incubation in vitro. 5 It is concluded that prostaglandins do not have an exclusive role in the mediation of the hypersensitivity reaction."}
{"id": "PMID:465894", "title": "Backward walking and circling: behavioural responses induced by drug treatments which cause simultaneous release of catecholamines and 5-hydroxytryptamine.", "content": "1 The roles of catecholamine and 5-hydroxytryptamine (5-HT) release in mediating backward walking and circling were studied in rats. 2 These behaviours occurred in animals given 15 mg/kg intraperitoneally of (+)-amphetamine (which predominantly releases catecholamines) or either p-chloroamphetamine or fenfluramine (which predominantly release 5-HT). They also occurred when smaller doses of (+)-amphetamine (5 mg/kg) and either p-chloroamphetamine (2--5 mg/kg) or fenfluramine (5 mg/kg) were given together. 3 Characteristic dopamine-dependent behaviours (rearing, licking, gnawing) resulting from (+)-amphetamine injection were greatly reduced by p-chloroamphetamine or fenfluramine. 4 Characteristic 5-HT-dependent behaviours (wet dog shake, hind limb abduction) resulting from injection of either p-chloroamphetamine or fenfluramine were unaffected by (+)-amphetamine. 5 Fragmentary backward walking and circling resulting from levallorphan injection (50 mg/kg s.c.) were decreased by (+)-amphetamine at low dosage. 6 Results in general strengthen previous evidence that backward walking and circling are mediated by simultaneous dopamine and 5-HT release. 7 The possible relevance of the above findings to hallucinogenic activity, amphetamine psychosis, schizophrenia and abnormal movements due to L-DOPA treatment is discussed.", "contents": "Backward walking and circling: behavioural responses induced by drug treatments which cause simultaneous release of catecholamines and 5-hydroxytryptamine. 1 The roles of catecholamine and 5-hydroxytryptamine (5-HT) release in mediating backward walking and circling were studied in rats. 2 These behaviours occurred in animals given 15 mg/kg intraperitoneally of (+)-amphetamine (which predominantly releases catecholamines) or either p-chloroamphetamine or fenfluramine (which predominantly release 5-HT). They also occurred when smaller doses of (+)-amphetamine (5 mg/kg) and either p-chloroamphetamine (2--5 mg/kg) or fenfluramine (5 mg/kg) were given together. 3 Characteristic dopamine-dependent behaviours (rearing, licking, gnawing) resulting from (+)-amphetamine injection were greatly reduced by p-chloroamphetamine or fenfluramine. 4 Characteristic 5-HT-dependent behaviours (wet dog shake, hind limb abduction) resulting from injection of either p-chloroamphetamine or fenfluramine were unaffected by (+)-amphetamine. 5 Fragmentary backward walking and circling resulting from levallorphan injection (50 mg/kg s.c.) were decreased by (+)-amphetamine at low dosage. 6 Results in general strengthen previous evidence that backward walking and circling are mediated by simultaneous dopamine and 5-HT release. 7 The possible relevance of the above findings to hallucinogenic activity, amphetamine psychosis, schizophrenia and abnormal movements due to L-DOPA treatment is discussed."}
{"id": "PMID:465895", "title": "Temperature coefficients of affinity and entropies of adsorption from enantiomeric pairs of compounds acting at muscarinic receptors in the guinea-pig ileum.", "content": "1 The affinities of a series of enantiomeric pairs of esters of phenylcyclohexylglycollic acid, of a pair of esters of alpha-methyltropic acid and of hyoscyamine methiodide have been measured for the muscarinic receptors of the guinea-pig ileum at 30 degrees and 37 degrees C and estimates have been made of their free energies, enthalpies and entropies of adsorption. 2 With (-)-S-hyoscyamine methiodide the enthalpy of adsorption is negative whereas with the (+)-R-enantiomer it is positive. 3 With the esters of phenylcyclohexylglycollic acid the size of the onium group appears to be as important as the stereochemical configuration in determining entropy. Large onium groups appear to be associated with an increase in entropy though this can only be measured approximately because of the narrow range of temperature which can be used.", "contents": "Temperature coefficients of affinity and entropies of adsorption from enantiomeric pairs of compounds acting at muscarinic receptors in the guinea-pig ileum. 1 The affinities of a series of enantiomeric pairs of esters of phenylcyclohexylglycollic acid, of a pair of esters of alpha-methyltropic acid and of hyoscyamine methiodide have been measured for the muscarinic receptors of the guinea-pig ileum at 30 degrees and 37 degrees C and estimates have been made of their free energies, enthalpies and entropies of adsorption. 2 With (-)-S-hyoscyamine methiodide the enthalpy of adsorption is negative whereas with the (+)-R-enantiomer it is positive. 3 With the esters of phenylcyclohexylglycollic acid the size of the onium group appears to be as important as the stereochemical configuration in determining entropy. Large onium groups appear to be associated with an increase in entropy though this can only be measured approximately because of the narrow range of temperature which can be used."}
{"id": "PMID:465896", "title": "Temperature coefficients of affinity constants for the binding of antagonists to muscarinic receptors in the rat cerebral cortex.", "content": "1 The temperature coefficients of binding of a series of muscarinic antagonists to their receptors in membrane preparations from the rat cerebral cortex has been examined. 2 At 37 degrees C the affinity constants agree with those determined by antagonism of acetylcholine-induced contractions of the guinea-pig ileum. 3 The temperature-dependence of the affinity constants is low; for the antagonists examined, the affinity constants at 0 degrees C differ by less than a factor of 3 from those measured at 37 degrees C. 4 There are qualitative similarities between the estimates of the temperature coefficient of binding of the antagonists to receptors in ileum strips and in the cerebral cortex.", "contents": "Temperature coefficients of affinity constants for the binding of antagonists to muscarinic receptors in the rat cerebral cortex. 1 The temperature coefficients of binding of a series of muscarinic antagonists to their receptors in membrane preparations from the rat cerebral cortex has been examined. 2 At 37 degrees C the affinity constants agree with those determined by antagonism of acetylcholine-induced contractions of the guinea-pig ileum. 3 The temperature-dependence of the affinity constants is low; for the antagonists examined, the affinity constants at 0 degrees C differ by less than a factor of 3 from those measured at 37 degrees C. 4 There are qualitative similarities between the estimates of the temperature coefficient of binding of the antagonists to receptors in ileum strips and in the cerebral cortex."}
{"id": "PMID:465897", "title": "Effects of methoxyverapamil on the stimulation by Ca2+, Sr2+ and Ba2+ and on the inhibition by Mg2+ of catecholamine release from the adrenal medulla.", "content": "1 Bovine adrenal glands were perfused with Ca2+-free Locke solution and catecholamine release was induced either by the introduction of Ca2+, Sr2+ or Ba2+ into the perfusion fluid or by the substitution of Na+ by an osmotically equivalent amount of sucrose. 2 Methoxyverapamil (D600) at a concentration of 3 X 10(-4) M blocked the release of catecholamines in response to Ca2+, Sr2+ or Ba2+ stimulation but failed to block the release evoked by the omission of Na+. 3 Mg2+ (10 to 20 mM) blocked the release induced by Na+-deprivation; however, this inhibitory effect of Mg2+ was not modified by D600. 4 D600 blocked the increase in the efflux of 45Ca from the perfused gland induced by the introduction of Ca2+ into the perfusion fluid and blocked the uptake of 45Ca into adrenal medullary slices induced by K+ depolarization. 5 The results suggest that Ca2+, Sr2+ and Ba2+ may enter the chromaffin cell through the same channel and that this channel is blocked by D600. Mg2+ may enter the cell through the same Ca2+ channel but with a high rate of permeation or it may enter through a channel which is resistant to D600. Alternatively, Mg2+ may exert this inhibitory effect at an extracellular site.", "contents": "Effects of methoxyverapamil on the stimulation by Ca2+, Sr2+ and Ba2+ and on the inhibition by Mg2+ of catecholamine release from the adrenal medulla. 1 Bovine adrenal glands were perfused with Ca2+-free Locke solution and catecholamine release was induced either by the introduction of Ca2+, Sr2+ or Ba2+ into the perfusion fluid or by the substitution of Na+ by an osmotically equivalent amount of sucrose. 2 Methoxyverapamil (D600) at a concentration of 3 X 10(-4) M blocked the release of catecholamines in response to Ca2+, Sr2+ or Ba2+ stimulation but failed to block the release evoked by the omission of Na+. 3 Mg2+ (10 to 20 mM) blocked the release induced by Na+-deprivation; however, this inhibitory effect of Mg2+ was not modified by D600. 4 D600 blocked the increase in the efflux of 45Ca from the perfused gland induced by the introduction of Ca2+ into the perfusion fluid and blocked the uptake of 45Ca into adrenal medullary slices induced by K+ depolarization. 5 The results suggest that Ca2+, Sr2+ and Ba2+ may enter the chromaffin cell through the same channel and that this channel is blocked by D600. Mg2+ may enter the cell through the same Ca2+ channel but with a high rate of permeation or it may enter through a channel which is resistant to D600. Alternatively, Mg2+ may exert this inhibitory effect at an extracellular site."}
{"id": "PMID:465898", "title": "The effect of aminosteroid, ORG 6001, on hypothermia induced ventricular fibrillation in the cat.", "content": "1 The effect of the antidysrhythmic aminosteroid, ORG 6001, on hypothermia-induced ventricular fibrillation was investigated in cats anaesthetized with pentobarbitone. 2 ORG 6001 (total dose, 10 mg/kg, by intravenous injection) reduced both the incidence of fibrillation and the temperature at which it occurred. The number of animals that survived to 16 degrees C was increased. 3 This protective effect of ORG 6001 could not be explained by changes in respiratory acidosis, plasma concentrations of sodium and potassium, or by changes in the action potential of excised hypothermic ventricular muscle. The hypothermia-induced elevation of blood lactate was less in cats treated with the aminosteroid. 4 Over a limited temperature range, ORG 6001 prolonged the P wave and QRS duration and shortened the QTc interval. ST segment elevation was slightly reduced in the drug-treated group. J deflections were observed but were not correlated with the development of fibrillation. 5 The onset of fibrillation was not considered to be due to temperature differences between the myocardium and arterial blood or between localized areas of the left ventricular wall.", "contents": "The effect of aminosteroid, ORG 6001, on hypothermia induced ventricular fibrillation in the cat. 1 The effect of the antidysrhythmic aminosteroid, ORG 6001, on hypothermia-induced ventricular fibrillation was investigated in cats anaesthetized with pentobarbitone. 2 ORG 6001 (total dose, 10 mg/kg, by intravenous injection) reduced both the incidence of fibrillation and the temperature at which it occurred. The number of animals that survived to 16 degrees C was increased. 3 This protective effect of ORG 6001 could not be explained by changes in respiratory acidosis, plasma concentrations of sodium and potassium, or by changes in the action potential of excised hypothermic ventricular muscle. The hypothermia-induced elevation of blood lactate was less in cats treated with the aminosteroid. 4 Over a limited temperature range, ORG 6001 prolonged the P wave and QRS duration and shortened the QTc interval. ST segment elevation was slightly reduced in the drug-treated group. J deflections were observed but were not correlated with the development of fibrillation. 5 The onset of fibrillation was not considered to be due to temperature differences between the myocardium and arterial blood or between localized areas of the left ventricular wall."}
{"id": "PMID:465899", "title": "The energy cost of an 80 km run.", "content": "Data was collected from two men who attempted an 80 km run. Measurements of aerobic power (VO2 max) and determinations of heart rate (HR) and submaximal oxygen consumption (VO2) during treadmill running were carried out one week before the run. Throughout the 80 km run, HR was recorded by telemetry and used together with the laboratory data to estimate VO2 as a percentage of VO2 max. One subject completed the 80 km distance at 58% of VO2 max, the other subject, operating at 74% of VO2 max, was obliged to retired after 55 km. The data in this and other studies indicate that the high energy costs reported for the marathon (70-85% of VO2 max) cannot be sustained over the 80 km distance but that about 60% of VO2 max can be continued for seven hours and longer.", "contents": "The energy cost of an 80 km run. Data was collected from two men who attempted an 80 km run. Measurements of aerobic power (VO2 max) and determinations of heart rate (HR) and submaximal oxygen consumption (VO2) during treadmill running were carried out one week before the run. Throughout the 80 km run, HR was recorded by telemetry and used together with the laboratory data to estimate VO2 as a percentage of VO2 max. One subject completed the 80 km distance at 58% of VO2 max, the other subject, operating at 74% of VO2 max, was obliged to retired after 55 km. The data in this and other studies indicate that the high energy costs reported for the marathon (70-85% of VO2 max) cannot be sustained over the 80 km distance but that about 60% of VO2 max can be continued for seven hours and longer."}
{"id": "PMID:465901", "title": "Body composition of rugby union football players.", "content": "The components of body composition were studied in 56 second class rugby union football players: 28 forwards and 28 backs. Fowards were found to have 19.5% TBF (TOTAL BODY FAT) and 80.5% LBM (LEAN BODY MASS); backs 12.2% TBF and 87.8% LBM. It was felt that greater attention should be given to the ratio of TBF to LBM in determining body weight and that there should be an increased emphasis of the LBM at the expense of TBF.", "contents": "Body composition of rugby union football players. The components of body composition were studied in 56 second class rugby union football players: 28 forwards and 28 backs. Fowards were found to have 19.5% TBF (TOTAL BODY FAT) and 80.5% LBM (LEAN BODY MASS); backs 12.2% TBF and 87.8% LBM. It was felt that greater attention should be given to the ratio of TBF to LBM in determining body weight and that there should be an increased emphasis of the LBM at the expense of TBF."}
{"id": "PMID:465902", "title": "A study of maximum oxygen uptake and heart rate during work and recovery as measured on cycle ergometer on national Indian sportsmen.", "content": "We have studied 73, top level Indian sportsmen for VO2 max and heart rates during work and recovery. High negative values of 'r' between VO2 max and heart rate during work and recovery have been observed. Absolute VO2 max, VO2 max/kg body wt/min and VO2 max/kg lean body mass/min are found to play an important role in influencing recovery of heart rate, more so in the initial phases. A similar trend has been observed while studying the influences of work heart rate on the recovery of heart rate after a standard exercise. Regression equations for the prediction of VO2 max from recovery pulse scores, have been worked out for Indian sportsmen.", "contents": "A study of maximum oxygen uptake and heart rate during work and recovery as measured on cycle ergometer on national Indian sportsmen. We have studied 73, top level Indian sportsmen for VO2 max and heart rates during work and recovery. High negative values of 'r' between VO2 max and heart rate during work and recovery have been observed. Absolute VO2 max, VO2 max/kg body wt/min and VO2 max/kg lean body mass/min are found to play an important role in influencing recovery of heart rate, more so in the initial phases. A similar trend has been observed while studying the influences of work heart rate on the recovery of heart rate after a standard exercise. Regression equations for the prediction of VO2 max from recovery pulse scores, have been worked out for Indian sportsmen."}
{"id": "PMID:465903", "title": "Equestrian injuries--a one year prospective study.", "content": "A one year prospective study of equestrian injuries was carried out in an area where horse riding is a popular pursuit. 115 persons suffered injury--eighty females and thirty-five males of whom sixty were under fifteen years of age. No fatalities were recorded and there were 0.2 injuries per 100 rides. Most injuries were minor and to the musculo-skeletal system. Visceral and cerebral injuries were not common but the former were life threatening in all cases. It is concluded that young riders should be supervised.", "contents": "Equestrian injuries--a one year prospective study. A one year prospective study of equestrian injuries was carried out in an area where horse riding is a popular pursuit. 115 persons suffered injury--eighty females and thirty-five males of whom sixty were under fifteen years of age. No fatalities were recorded and there were 0.2 injuries per 100 rides. Most injuries were minor and to the musculo-skeletal system. Visceral and cerebral injuries were not common but the former were life threatening in all cases. It is concluded that young riders should be supervised."}
{"id": "PMID:465907", "title": "Chronic respiratory failure and physical reconditioning: case study of an elderly obese woman.", "content": "A case is described of a 67 year old obese white woman who had a history of multiple medical problems and who was in chronic respiratory failure but responded poorly to intermittent positive pressure breathing, chest physiotherapy, and supplementary oxygen. She was treated successfully with a 600 k.cal diet and a 26-day physical reconditioning programme. Reconditioning techniques included free and treadmill walking, stair climbing, bench stepping, light calisthenics, and breathing retraining. Improvements were noticed in blood gases, spirometry, electrocardiogram, motor coordination, and physical working capacity.", "contents": "Chronic respiratory failure and physical reconditioning: case study of an elderly obese woman. A case is described of a 67 year old obese white woman who had a history of multiple medical problems and who was in chronic respiratory failure but responded poorly to intermittent positive pressure breathing, chest physiotherapy, and supplementary oxygen. She was treated successfully with a 600 k.cal diet and a 26-day physical reconditioning programme. Reconditioning techniques included free and treadmill walking, stair climbing, bench stepping, light calisthenics, and breathing retraining. Improvements were noticed in blood gases, spirometry, electrocardiogram, motor coordination, and physical working capacity."}
{"id": "PMID:465909", "title": "The treatment of iliotibial band friction syndrome.", "content": "Iliotibial band friction syndrome is a common cause of knee pain in long distance runners. The pain is caused by friction of the iliotibial band over the lateral epicondyle of the femur. Two hundred and twenty one cases were seen in a two year period. Tenderness over the lateral epicondyle associated with pain at 30 degrees of flexion on compressing the iliotibial band against the lateral epicondyle is diagnostic. Conservative treatment consisted of treating the cause, mostly training irregularities and the local inflammation mostly with steroid injections and rest. Nine cases failed to respond to conservative treatment and these were treated by surgical release of the posterior fibres of the iliotibial band.", "contents": "The treatment of iliotibial band friction syndrome. Iliotibial band friction syndrome is a common cause of knee pain in long distance runners. The pain is caused by friction of the iliotibial band over the lateral epicondyle of the femur. Two hundred and twenty one cases were seen in a two year period. Tenderness over the lateral epicondyle associated with pain at 30 degrees of flexion on compressing the iliotibial band against the lateral epicondyle is diagnostic. Conservative treatment consisted of treating the cause, mostly training irregularities and the local inflammation mostly with steroid injections and rest. Nine cases failed to respond to conservative treatment and these were treated by surgical release of the posterior fibres of the iliotibial band."}
{"id": "PMID:465911", "title": "Determinations of performance and mechanical efficiency in nordic skiing.", "content": "Determinants of performance and mechanical efficiency of effort have been made on a group of ten male nordic skiers, all participants in the University of Toronto ski-team. The oxygen intake at the maximum attainable speed of skiing on a level course averaged 89.6 percent of the maximum oxygen intake observed during uphill treadmill running; the latter (average 63.9 ml.kg-1 min-1) may be compared with values greater than 80 ml.kg1 min-1 for international competitors. Maximum heart rates and respiratory gas exchange ratios were generally lower during skiing than running, and it is suggested that the maximum oxygen intake attained during skiing is limited by the individual's skill. In support of this the more experienced skiers were able to reach close to 100 percent of the treadmill maximum oxygen intake during level skiing. A multiple regression analysis indicated that the skiing speed sustained over a one-hour period was related to experience of skiing, maximum oxygen intake, and the percentage of body fat. Assuming a dynamic friction coefficient of 0.075, a drag area of 0.7 m2 and a drag coefficient of 1.0, the gross mechanical efficiency of the university-class skier averaged a little under 20 percent, with a net efficiency of 21.3 percent.", "contents": "Determinations of performance and mechanical efficiency in nordic skiing. Determinants of performance and mechanical efficiency of effort have been made on a group of ten male nordic skiers, all participants in the University of Toronto ski-team. The oxygen intake at the maximum attainable speed of skiing on a level course averaged 89.6 percent of the maximum oxygen intake observed during uphill treadmill running; the latter (average 63.9 ml.kg-1 min-1) may be compared with values greater than 80 ml.kg1 min-1 for international competitors. Maximum heart rates and respiratory gas exchange ratios were generally lower during skiing than running, and it is suggested that the maximum oxygen intake attained during skiing is limited by the individual's skill. In support of this the more experienced skiers were able to reach close to 100 percent of the treadmill maximum oxygen intake during level skiing. A multiple regression analysis indicated that the skiing speed sustained over a one-hour period was related to experience of skiing, maximum oxygen intake, and the percentage of body fat. Assuming a dynamic friction coefficient of 0.075, a drag area of 0.7 m2 and a drag coefficient of 1.0, the gross mechanical efficiency of the university-class skier averaged a little under 20 percent, with a net efficiency of 21.3 percent."}
{"id": "PMID:465912", "title": "Physical efficiency tests in Indian urban adolescent boys and girls.", "content": "Simple anthropometric measurements and physical efficiency tests were performed on adolescent Indian urban boys between 10-14 years and girls between 11-14 years of age. The similar height and weight of the subjects of both sexes were observed and was suggestive of higher growth rate in girls than the boys during only the initial phase of adolescent growth spurt. The vital capacity (total, ml/cm of height, l/m2 of surface area), Peak Expiratory Flow Rate (total, l/cm of height, l/kg of weight, l/m2 of surface area, l/year of age) and grip strength were observed to be insignificantly different between the sexes.", "contents": "Physical efficiency tests in Indian urban adolescent boys and girls. Simple anthropometric measurements and physical efficiency tests were performed on adolescent Indian urban boys between 10-14 years and girls between 11-14 years of age. The similar height and weight of the subjects of both sexes were observed and was suggestive of higher growth rate in girls than the boys during only the initial phase of adolescent growth spurt. The vital capacity (total, ml/cm of height, l/m2 of surface area), Peak Expiratory Flow Rate (total, l/cm of height, l/kg of weight, l/m2 of surface area, l/year of age) and grip strength were observed to be insignificantly different between the sexes."}
{"id": "PMID:465913", "title": "Fitness test profiles and trainng intensities in skilled race-walkers.", "content": "A broad profile of national standard race-walkers was obtained. Subjects were taller and had more body fat than competitive runners of comparable distance as found in the literature. Pulmonary function, blood pressure and maximal heart rates were similar to normal sedentary values. The group's somatotype was 2.5 : 3 : 4, low mesomorphy being reflected in inferior strength measures. Haematological status corresponded to the runners of Brotherhood et al (1975). Predicted VO2 max (x = 70 ml kg min-1) was not related to performance. Time to exhaustion on a treadmill test correlated with 20 km race time (R = -.94; p less than .001). Multiple regression equations derived to predict race performance from combinations of 4 to 6 personality traits were non-significant. Mean heart rate in typical training regimes was 167 beats min-1 for interval training at 13 kmh-1 on the track and 134 beats min-1 over a 2.1 h road walk at 10.3 kmh-1. Physiological strain was greater in uphill than in level or downhill walking (P less than .001).", "contents": "Fitness test profiles and trainng intensities in skilled race-walkers. A broad profile of national standard race-walkers was obtained. Subjects were taller and had more body fat than competitive runners of comparable distance as found in the literature. Pulmonary function, blood pressure and maximal heart rates were similar to normal sedentary values. The group's somatotype was 2.5 : 3 : 4, low mesomorphy being reflected in inferior strength measures. Haematological status corresponded to the runners of Brotherhood et al (1975). Predicted VO2 max (x = 70 ml kg min-1) was not related to performance. Time to exhaustion on a treadmill test correlated with 20 km race time (R = -.94; p less than .001). Multiple regression equations derived to predict race performance from combinations of 4 to 6 personality traits were non-significant. Mean heart rate in typical training regimes was 167 beats min-1 for interval training at 13 kmh-1 on the track and 134 beats min-1 over a 2.1 h road walk at 10.3 kmh-1. Physiological strain was greater in uphill than in level or downhill walking (P less than .001)."}
{"id": "PMID:465917", "title": "The radiological manifestations of metaphyseal dysplasia (Pyle disease).", "content": "Pyle disease is a rare genetic skeletal disorder which is conventionally classified with craniotubular dysplasias. The radiographic manifestations in three affected adults included widening of the metaphyseal portions of the long bones which extended through a major portion of the diaphyses, with cortical thinning and mild cranial sclerosis. The femora presented the characteristic Erlenmeyer flask configuration. Pyle disease is clinically, radiographically and genetically distinct from craniometaphyseal dysplasia, a relatively common condition with which it has been confused.", "contents": "The radiological manifestations of metaphyseal dysplasia (Pyle disease). Pyle disease is a rare genetic skeletal disorder which is conventionally classified with craniotubular dysplasias. The radiographic manifestations in three affected adults included widening of the metaphyseal portions of the long bones which extended through a major portion of the diaphyses, with cortical thinning and mild cranial sclerosis. The femora presented the characteristic Erlenmeyer flask configuration. Pyle disease is clinically, radiographically and genetically distinct from craniometaphyseal dysplasia, a relatively common condition with which it has been confused."}
{"id": "PMID:465918", "title": "Metaphyseal fractures in osteogenesis imperfecta.", "content": "Forty-one children with osteogenesis imperfecta have been reviewed. A minority (7/41) showed small metaphyseal fractures, resembling those seen in non-accidental injury, but in all of these there was obvious generalized bone disease so that confusion with non-accidental injury did not occur.", "contents": "Metaphyseal fractures in osteogenesis imperfecta. Forty-one children with osteogenesis imperfecta have been reviewed. A minority (7/41) showed small metaphyseal fractures, resembling those seen in non-accidental injury, but in all of these there was obvious generalized bone disease so that confusion with non-accidental injury did not occur."}
{"id": "PMID:465919", "title": "A review of the radiological anatomy of the round window.", "content": "Experimental studies on dried temporal bones in various projections combined with studies of the anatomical details marked with metal wires and/or barium paste have been performed to define the round window and its anatomical relation to surrounding structures. The exact plane of the round window was not found to be tomographically demonstrable and the term to describe the dehiscence in the lateral wall of the promontory below and posterior to that of the oval window should be the niche of the round window. Previous radiologically described pathological changes at the round window should be reviewed.", "contents": "A review of the radiological anatomy of the round window. Experimental studies on dried temporal bones in various projections combined with studies of the anatomical details marked with metal wires and/or barium paste have been performed to define the round window and its anatomical relation to surrounding structures. The exact plane of the round window was not found to be tomographically demonstrable and the term to describe the dehiscence in the lateral wall of the promontory below and posterior to that of the oval window should be the niche of the round window. Previous radiologically described pathological changes at the round window should be reviewed."}
{"id": "PMID:465920", "title": "Intravenous cholangiography in childhood.", "content": "Intravenous cholangiography (IVC) was performed on 102 children aged between one and 13 years. Eighty of the patients who were non-icteric were divided into four groups and received contrast medium containing either 53, 75, 106 or 150 mg iodine/kg body weight by intravenous injection over ten minutes. Complete demonstration of the common bile duct (CBD) was achieved in 85% of patients and in a similar proportion of all age groups. There was some delay in opacification of the CBD in young children but this was not of practical significance. The two higher dosage regimen gave significantly better results than the two lower dosages (p less than 0.001) and this improvement was evident in the young and older age groups. Nausea and vomiting occurred as side effects in 6%. In eight patients in whom there was inadequate demonstration by the standard IVC, an infusion technique over 3--8 hours was carried out. This improved the demonstration in three children with diagnostic results in two.", "contents": "Intravenous cholangiography in childhood. Intravenous cholangiography (IVC) was performed on 102 children aged between one and 13 years. Eighty of the patients who were non-icteric were divided into four groups and received contrast medium containing either 53, 75, 106 or 150 mg iodine/kg body weight by intravenous injection over ten minutes. Complete demonstration of the common bile duct (CBD) was achieved in 85% of patients and in a similar proportion of all age groups. There was some delay in opacification of the CBD in young children but this was not of practical significance. The two higher dosage regimen gave significantly better results than the two lower dosages (p less than 0.001) and this improvement was evident in the young and older age groups. Nausea and vomiting occurred as side effects in 6%. In eight patients in whom there was inadequate demonstration by the standard IVC, an infusion technique over 3--8 hours was carried out. This improved the demonstration in three children with diagnostic results in two."}
{"id": "PMID:465921", "title": "Traumatic para-mediastinal air cysts.", "content": "Two cases of traumatic para-mediastinal air cyst are presented. The air and fluid collection develops between two layers of the pulmonary ligament following trauma to the lung and rupture of alveoli adjacent to the pulmonary ligament.", "contents": "Traumatic para-mediastinal air cysts. Two cases of traumatic para-mediastinal air cyst are presented. The air and fluid collection develops between two layers of the pulmonary ligament following trauma to the lung and rupture of alveoli adjacent to the pulmonary ligament."}
{"id": "PMID:465922", "title": "Kidney distension during IV urography in normal rats and in rats with artificial unilateral renal artery stenosis.", "content": "Acute changes in kidney size during IV urography were studied in normal rats and in rats with artificial unilateral renal artery stenosis. In normal rats, IV bolus injection of sodium iothalamate (400 mg I/ml), 1.5 ml/kg body weight, produced an increase in area of the renal silhouette by an average maximum of 12.7% after 60 seconds. In nine out of ten rats which developed hypertension after left sided renal artery clamping, renal distension was either small or absent on the stenosed side, while the contralateral right kidney showed normal distension during IV urography. Two out of three rats, which remained normotensive after renal arterial clamping, showed normal distension of both kidneys. These results confirm earlier observations in man that the acute changes in size of the kidney during IV urography can be used as an aid in the urographic screening of hypertensive patients for significant renal arterial stenosis. In all rats with arterial clamp and hypertension, the stenosed kidney was smaller than its contralateral mate. An index between the size of the non-stenosed and the stenosed kidneys greater than 1.15 was only seen in these particular rats.", "contents": "Kidney distension during IV urography in normal rats and in rats with artificial unilateral renal artery stenosis. Acute changes in kidney size during IV urography were studied in normal rats and in rats with artificial unilateral renal artery stenosis. In normal rats, IV bolus injection of sodium iothalamate (400 mg I/ml), 1.5 ml/kg body weight, produced an increase in area of the renal silhouette by an average maximum of 12.7% after 60 seconds. In nine out of ten rats which developed hypertension after left sided renal artery clamping, renal distension was either small or absent on the stenosed side, while the contralateral right kidney showed normal distension during IV urography. Two out of three rats, which remained normotensive after renal arterial clamping, showed normal distension of both kidneys. These results confirm earlier observations in man that the acute changes in size of the kidney during IV urography can be used as an aid in the urographic screening of hypertensive patients for significant renal arterial stenosis. In all rats with arterial clamp and hypertension, the stenosed kidney was smaller than its contralateral mate. An index between the size of the non-stenosed and the stenosed kidneys greater than 1.15 was only seen in these particular rats."}
{"id": "PMID:465923", "title": "Doppler ultrasound examination of pathologically enlarged lymph nodes.", "content": "Pathologically enlarged lymph nodes have been examined with a commercially available 10 MHz continuous-wave Doppler flowmeter. Many enlarged lymph nodes gave rise to significant Doppler-shift signals indicating increased blood flow. The signals have been spectrum analysed and the large diastolic flow components suggest that there is considerable arterio-venous shunting within lymph glands involved in leukemia, lymphoma and carcinoma. It also seems that the signals tend to diminish in response to treatment. The Doppler signals have been used in an imaging system to produce a vascular map of the region of the enlarged gland. It is suggested that these findings might be applicable to the detection of neoplastic tissues in less accessible sites.", "contents": "Doppler ultrasound examination of pathologically enlarged lymph nodes. Pathologically enlarged lymph nodes have been examined with a commercially available 10 MHz continuous-wave Doppler flowmeter. Many enlarged lymph nodes gave rise to significant Doppler-shift signals indicating increased blood flow. The signals have been spectrum analysed and the large diastolic flow components suggest that there is considerable arterio-venous shunting within lymph glands involved in leukemia, lymphoma and carcinoma. It also seems that the signals tend to diminish in response to treatment. The Doppler signals have been used in an imaging system to produce a vascular map of the region of the enlarged gland. It is suggested that these findings might be applicable to the detection of neoplastic tissues in less accessible sites."}
{"id": "PMID:465924", "title": "The effect on dosimetry of changing from the lithotomy to the supine position in treating uterine cancer with the Cathetron and external radiotherapy.", "content": "Uterine carcinoma can be treated by combined external and intracavitary irradiation. With an afterloading technique intracavitary irradiation is given with the patients in the lithotomy position. External irradiation is given with the patient in the supine position. A lead shield is used with the external fields to cover the tissues which are close to the intracavitary sources and receive high doses. The positions of the intracavitary sources seen on radiographs taken when the patient is in the lithotomy position are transferred to films taken with the patient in the supine position. From the latter the position and profile of the shield are calculated. It is shown that a reliable transfer of the positions of the sources and determination of the necessary shielding cannot be achieved in this way. It is therefore concluded that both intracavitary and external irradiation should be carried out with the patient in the supine position.", "contents": "The effect on dosimetry of changing from the lithotomy to the supine position in treating uterine cancer with the Cathetron and external radiotherapy. Uterine carcinoma can be treated by combined external and intracavitary irradiation. With an afterloading technique intracavitary irradiation is given with the patients in the lithotomy position. External irradiation is given with the patient in the supine position. A lead shield is used with the external fields to cover the tissues which are close to the intracavitary sources and receive high doses. The positions of the intracavitary sources seen on radiographs taken when the patient is in the lithotomy position are transferred to films taken with the patient in the supine position. From the latter the position and profile of the shield are calculated. It is shown that a reliable transfer of the positions of the sources and determination of the necessary shielding cannot be achieved in this way. It is therefore concluded that both intracavitary and external irradiation should be carried out with the patient in the supine position."}
{"id": "PMID:465925", "title": "A computerized three-dimensional treatment planning system utilizing interactive colour graphics.", "content": "A new computerized radiation treatment planning system has been developed to aid in three-dimensional treatment planning. Using interactive colour graphics in conjunction with a DPD 11/45 computer, the system can take multiple transverse contours and construct a perspective display of the treatment region showing organ surfaces as well as cross-sectional contours. With interactively selected orientations, the display allows easy perception of the relative positioning of the treatment volume and neighbouring anatomy. For external beam treatment planning, interactive computer simulation is used to select diaphragm sizes which best conform to the target area while avoiding sensitive structures. Dose calculations for the selected beams are carried out on multiple transverse planes. The calculational planes and surfaces are displayed in perspective with radiation dosage displayed in an interactively manipulated colour display. Altogether the system provides an easy assessment of the volume to be irradiated, interactive selection of optimal arrangements of treatment fields and a means for visualizing and evaluating the resulting dose distributions.", "contents": "A computerized three-dimensional treatment planning system utilizing interactive colour graphics. A new computerized radiation treatment planning system has been developed to aid in three-dimensional treatment planning. Using interactive colour graphics in conjunction with a DPD 11/45 computer, the system can take multiple transverse contours and construct a perspective display of the treatment region showing organ surfaces as well as cross-sectional contours. With interactively selected orientations, the display allows easy perception of the relative positioning of the treatment volume and neighbouring anatomy. For external beam treatment planning, interactive computer simulation is used to select diaphragm sizes which best conform to the target area while avoiding sensitive structures. Dose calculations for the selected beams are carried out on multiple transverse planes. The calculational planes and surfaces are displayed in perspective with radiation dosage displayed in an interactively manipulated colour display. Altogether the system provides an easy assessment of the volume to be irradiated, interactive selection of optimal arrangements of treatment fields and a means for visualizing and evaluating the resulting dose distributions."}
{"id": "PMID:465926", "title": "The dose in water surrounding point isotropic gamma-ray emitters.", "content": "A Monte Carlo method has been used to calculate the dose distribution around point gamma-ray emitters in water. Five gamma-ray emitting radionuclide, 60Co, 137Cs, 192Ir, 198Au and 226Ra, which are commonly used in radiotherapy interstitial implants have been considered. The results are in substantial agreement with those derived using a combination of experimental and theoretical data.", "contents": "The dose in water surrounding point isotropic gamma-ray emitters. A Monte Carlo method has been used to calculate the dose distribution around point gamma-ray emitters in water. Five gamma-ray emitting radionuclide, 60Co, 137Cs, 192Ir, 198Au and 226Ra, which are commonly used in radiotherapy interstitial implants have been considered. The results are in substantial agreement with those derived using a combination of experimental and theoretical data."}
{"id": "PMID:465927", "title": "Lack of correlation between villus and crypt damage in irradiated mouse intestine.", "content": "It has been observed that scanning electron microscopy is a more sensitive indicator of mucosal damage at low radiation dose levels than conventional quantitative crypt counting techniques. Three different fractionation schedules were subjected to investigation by both of these methods to try to elucidate some features of irradiation damage to the whole of the intestinal mucosa, at dose levels commonly used in clinical practice. Despite variations in the qualitative observations, there was a marked difference in two of the schedules between damage expressed as crypt counts and that described by the qualitative techniques. In the first case high crypt numbers were associated with severe mucosal damage, whereas the second schedule produced a reduced crypt count in association with low damage to the surface mucosa. A third schedule produced results in which there was a general agreement between low crypt numbers and considerable surface mucosal damage. However, observations were made of mucosal formations not previously seen on damaged mucosal surfaces. These resembled the appearance normally associated with the gut of patients suffering from coeliac disease. Variations in the qualitative observations were seen in all the schedules so that their interpretation in terms of perturbation of cellular kinetics is difficult.", "contents": "Lack of correlation between villus and crypt damage in irradiated mouse intestine. It has been observed that scanning electron microscopy is a more sensitive indicator of mucosal damage at low radiation dose levels than conventional quantitative crypt counting techniques. Three different fractionation schedules were subjected to investigation by both of these methods to try to elucidate some features of irradiation damage to the whole of the intestinal mucosa, at dose levels commonly used in clinical practice. Despite variations in the qualitative observations, there was a marked difference in two of the schedules between damage expressed as crypt counts and that described by the qualitative techniques. In the first case high crypt numbers were associated with severe mucosal damage, whereas the second schedule produced a reduced crypt count in association with low damage to the surface mucosa. A third schedule produced results in which there was a general agreement between low crypt numbers and considerable surface mucosal damage. However, observations were made of mucosal formations not previously seen on damaged mucosal surfaces. These resembled the appearance normally associated with the gut of patients suffering from coeliac disease. Variations in the qualitative observations were seen in all the schedules so that their interpretation in terms of perturbation of cellular kinetics is difficult."}
{"id": "PMID:465928", "title": "OER and RBE for negative pion beams of different peak widths.", "content": "Experimental data on survival curves for pion because of different peak widths under aerobic and hypoxic conditions are reported. Metabolic depletion of oxygen by the Chinese hamster cells (line V79) was used to obtain hypoxia. The results indicate that the RBE at the beam entrance (plateau) is approximately 1.0. When the Bragg peaks were broadened to widths of 1.3, 7.8, and 10.5 cm (at the 80% dose level), the RBE (50% cell survival) at the peak centres was 1.7, 1.6, and 1.2, respectively. The OER at the entrance was 2.4 compared with about 2.9 for X rays. The OER was independent of the survival level at which it was measured. The OER at the peak centres at widths of 1.3, 7.8, and 10.5 cm was 2.1, 2.4, and 2.2, respectively. These results indicate that, although the RBE at the centre of the 10.5 cm wide peak was significantly lower than at the centres of the 1.3 and 7.8 cm peaks, the OER values are similar for all peak widths used in this study.", "contents": "OER and RBE for negative pion beams of different peak widths. Experimental data on survival curves for pion because of different peak widths under aerobic and hypoxic conditions are reported. Metabolic depletion of oxygen by the Chinese hamster cells (line V79) was used to obtain hypoxia. The results indicate that the RBE at the beam entrance (plateau) is approximately 1.0. When the Bragg peaks were broadened to widths of 1.3, 7.8, and 10.5 cm (at the 80% dose level), the RBE (50% cell survival) at the peak centres was 1.7, 1.6, and 1.2, respectively. The OER at the entrance was 2.4 compared with about 2.9 for X rays. The OER was independent of the survival level at which it was measured. The OER at the peak centres at widths of 1.3, 7.8, and 10.5 cm was 2.1, 2.4, and 2.2, respectively. These results indicate that, although the RBE at the centre of the 10.5 cm wide peak was significantly lower than at the centres of the 1.3 and 7.8 cm peaks, the OER values are similar for all peak widths used in this study."}
{"id": "PMID:465939", "title": "Selective angiography in renal and peri-renal inflammatory lesions: correlation with histopathology.", "content": "We have correlated the angiographic and pathological findings in renal and peri-renal inflammatory disease in acute, sub-acute and chronic phases. In all stages of renal infection, the renal vasculature is attenuated. This corresponds in acute inflammation to vasospasm, acute vascular necrosis and post glomerular peritubular capillary congestion. In sub-acute inflammation, the reduction in vascularity is due to peri-vascular fibrosis and intimal and medial hyperplasia. In chronic infections, the renal vessels are completely destroyed and replaced by fibrotic tissue. In contrast to this, peri-renal inflammatory disease leads to vaso-dilatation and enlargement of the renal capsular arterial complex, and of retroperitoneal arteries. If both renal and peri-renal inflammation occurs simultaneously, a discordance between the renal cortical and capsular arterial filling results: this striking phenomenon is highly suggestive of inflammation, and extremely rare in tumours. Three types of inflammatory vascularity are described, two of which can be easily distinguished from tumour vascularity. An inflammatory blush, slow emptying of vessels and a mottled nephrogram with loss of cortical definition are highly suggestive signs of renal inflammation. It is concluded that angiography is a sensitive and accurate method for the early diagnoses of renal and peri-renal flammatory conditions.", "contents": "Selective angiography in renal and peri-renal inflammatory lesions: correlation with histopathology. We have correlated the angiographic and pathological findings in renal and peri-renal inflammatory disease in acute, sub-acute and chronic phases. In all stages of renal infection, the renal vasculature is attenuated. This corresponds in acute inflammation to vasospasm, acute vascular necrosis and post glomerular peritubular capillary congestion. In sub-acute inflammation, the reduction in vascularity is due to peri-vascular fibrosis and intimal and medial hyperplasia. In chronic infections, the renal vessels are completely destroyed and replaced by fibrotic tissue. In contrast to this, peri-renal inflammatory disease leads to vaso-dilatation and enlargement of the renal capsular arterial complex, and of retroperitoneal arteries. If both renal and peri-renal inflammation occurs simultaneously, a discordance between the renal cortical and capsular arterial filling results: this striking phenomenon is highly suggestive of inflammation, and extremely rare in tumours. Three types of inflammatory vascularity are described, two of which can be easily distinguished from tumour vascularity. An inflammatory blush, slow emptying of vessels and a mottled nephrogram with loss of cortical definition are highly suggestive signs of renal inflammation. It is concluded that angiography is a sensitive and accurate method for the early diagnoses of renal and peri-renal flammatory conditions."}
{"id": "PMID:465940", "title": "Bronchial artery embolization in the control of massive haemoptysis.", "content": "Bronchial artery embolization is a useful palliative treatment for life-threatening haemoptyses where surgery is contra-indicated. Patients presenting with haemoptyses complicating cystic fibrosis and aspergilloma have been embolized. Control of haemoptysis was achieved in the former, but late recurrence of haemoptysis in the latter led to asphyxiation.", "contents": "Bronchial artery embolization in the control of massive haemoptysis. Bronchial artery embolization is a useful palliative treatment for life-threatening haemoptyses where surgery is contra-indicated. Patients presenting with haemoptyses complicating cystic fibrosis and aspergilloma have been embolized. Control of haemoptysis was achieved in the former, but late recurrence of haemoptysis in the latter led to asphyxiation."}
{"id": "PMID:465941", "title": "Pancreatic ultrasound--a systematic approach to scanning technique.", "content": "The pancreas remains one of the more difficult organs to visualize but ultrasound can be successful in nearly 90% of patients. A systematic technique for identifying and delineating the pancreas is described and has been verified on over 500 patients to date. The success of the technique depends on an accurate knowledge of the vascular relations of the pancreas and on correct adjustment of the ultrasound equipment. This paper is intended to help ultrasound practitioners achieve both these ends.", "contents": "Pancreatic ultrasound--a systematic approach to scanning technique. The pancreas remains one of the more difficult organs to visualize but ultrasound can be successful in nearly 90% of patients. A systematic technique for identifying and delineating the pancreas is described and has been verified on over 500 patients to date. The success of the technique depends on an accurate knowledge of the vascular relations of the pancreas and on correct adjustment of the ultrasound equipment. This paper is intended to help ultrasound practitioners achieve both these ends."}
{"id": "PMID:465942", "title": "NSD and cell survival.", "content": "The relation between the NSD concept and cell survival in fractionated irradiation is established; the slope of Strandquist's lines is related to the shape of survival curves for the given type of cells and radiation. A new parameter, CBE, derived from TDF for determining cumulative biological effect is proposed.", "contents": "NSD and cell survival. The relation between the NSD concept and cell survival in fractionated irradiation is established; the slope of Strandquist's lines is related to the shape of survival curves for the given type of cells and radiation. A new parameter, CBE, derived from TDF for determining cumulative biological effect is proposed."}
{"id": "PMID:465954", "title": "Urinary inhibitors of calcium phosphate formation: the inhibitory activity of normal and artificial urines.", "content": "Eelven urine samples from normal subjects inhibited the formation of calcium phosphate. In all cases the activity of the urine was identical with that of an artificial urine having the same concentration of urea, creatinine, sodium, potassium, magnesium, calcium, ammonia, sulphate, chloride, inorganic phosphate, citrate, isocitrate and pyrophosphate, and the same pH.", "contents": "Urinary inhibitors of calcium phosphate formation: the inhibitory activity of normal and artificial urines. Eelven urine samples from normal subjects inhibited the formation of calcium phosphate. In all cases the activity of the urine was identical with that of an artificial urine having the same concentration of urea, creatinine, sodium, potassium, magnesium, calcium, ammonia, sulphate, chloride, inorganic phosphate, citrate, isocitrate and pyrophosphate, and the same pH."}
{"id": "PMID:465955", "title": "Diuresis renography and morphology in upper urinary tract obstruction.", "content": "The results of a combined study of 25 patients with idiopathic hydronephrosis are presented. Diuresis renography was performed pre-operatively and surgically removed specimens of the renal pelvis and ureter were examined by light and electron microscopy. There was good correlation between the results of these 2 methods of assessment which have defined 2 broad categories of patients with renal pelvic dilatation. These findings are discussed with particular reference to the clinical value of diuresis renography.", "contents": "Diuresis renography and morphology in upper urinary tract obstruction. The results of a combined study of 25 patients with idiopathic hydronephrosis are presented. Diuresis renography was performed pre-operatively and surgically removed specimens of the renal pelvis and ureter were examined by light and electron microscopy. There was good correlation between the results of these 2 methods of assessment which have defined 2 broad categories of patients with renal pelvic dilatation. These findings are discussed with particular reference to the clinical value of diuresis renography."}
{"id": "PMID:465956", "title": "The intravenous urodynamogram.", "content": "Modifications to the intravenous urogram are described. It is suggested that the time has come to rationalise the standard procedure. If an evaluation of the lower urinary tract is required, the voiding films may be modified to provide an intranvenous urodynamogram. If an evaluation of the upper urinary tract is directed at a specific question, then the number of spot-films can often be reduced.", "contents": "The intravenous urodynamogram. Modifications to the intravenous urogram are described. It is suggested that the time has come to rationalise the standard procedure. If an evaluation of the lower urinary tract is required, the voiding films may be modified to provide an intranvenous urodynamogram. If an evaluation of the upper urinary tract is directed at a specific question, then the number of spot-films can often be reduced."}
{"id": "PMID:465957", "title": "The role of internal urethrotomy in the prevention of urethral stricture following transurethral resection of prostate.", "content": "Urethral stricture is the commonest late complication of transurethral prostatectomy. Although internal urethrotomy is widely practised to prevent structures, there are no reports of any controlled trials of the procedure. A prospective trial of internal urethrotomy, using the Otis urethrotome, in 210 consecutive transurethral prostatectomies is presented. Patients were divided into 2 groups, those undergoing urethrotomy before TUR (\"Trial group\") and those undergoing TUR without urethrotomy (\"Control group\"), and they were followed for a minimum of 6 months. The incidence of stricture in the control group was significantly greater than in the trial group (P less than 0.01). Analysis of all other variables revealed no difference between the 2 groups and it is concluded that internal urethrotomy does prevent stricture formation and that it should be undertaken routinely before transurethral resection.", "contents": "The role of internal urethrotomy in the prevention of urethral stricture following transurethral resection of prostate. Urethral stricture is the commonest late complication of transurethral prostatectomy. Although internal urethrotomy is widely practised to prevent structures, there are no reports of any controlled trials of the procedure. A prospective trial of internal urethrotomy, using the Otis urethrotome, in 210 consecutive transurethral prostatectomies is presented. Patients were divided into 2 groups, those undergoing urethrotomy before TUR (\"Trial group\") and those undergoing TUR without urethrotomy (\"Control group\"), and they were followed for a minimum of 6 months. The incidence of stricture in the control group was significantly greater than in the trial group (P less than 0.01). Analysis of all other variables revealed no difference between the 2 groups and it is concluded that internal urethrotomy does prevent stricture formation and that it should be undertaken routinely before transurethral resection."}
{"id": "PMID:465958", "title": "Conservative treatment of carcinoma of the penis.", "content": "Sixty-five cases referred to one hospital between 1964 and 1977 were studied. Forty-one presented in Stages 1 or 2 and when treated by the Iridium mould technique gave a 12/13 (92.3%) crude 5-year survival rate, an improvement over the results of previous methods of treatment, including partial amputation. In Stage 3 radical amputation gave an 89% 5-year survival rate. Node dissection was reserved for inguinal nodes in Stages 1, 2 and 3 which failed to resolve after treating the primary tumour. Undifferentiated tumours and those with the microscopic \"cord\" pattern of growth did badly.", "contents": "Conservative treatment of carcinoma of the penis. Sixty-five cases referred to one hospital between 1964 and 1977 were studied. Forty-one presented in Stages 1 or 2 and when treated by the Iridium mould technique gave a 12/13 (92.3%) crude 5-year survival rate, an improvement over the results of previous methods of treatment, including partial amputation. In Stage 3 radical amputation gave an 89% 5-year survival rate. Node dissection was reserved for inguinal nodes in Stages 1, 2 and 3 which failed to resolve after treating the primary tumour. Undifferentiated tumours and those with the microscopic \"cord\" pattern of growth did badly."}
{"id": "PMID:465959", "title": "Experience wiht vasovasostomy: operative technique and results.", "content": "Between 1972 and 1977 vasovasostomy to reverse a previous vasectomy for contraception has been attempted in 27 cases. The procedure was abandoned as a technical impossiblity in only 1 case. The first 17 have been studied and in these there have been 11 pregnancies, 10 of which have already come to term with the birth of normal children, including one set of twins. Of the remainder, 2 are known to have oligozoospermia and 4 have been lost to follow-up although 2 of these were euspermic when last heard of. In spite of the encouraging results it is considered that there are no grounds for altering the present basis of vasectomy counselling in which the operation is described as likely to be irreversible.", "contents": "Experience wiht vasovasostomy: operative technique and results. Between 1972 and 1977 vasovasostomy to reverse a previous vasectomy for contraception has been attempted in 27 cases. The procedure was abandoned as a technical impossiblity in only 1 case. The first 17 have been studied and in these there have been 11 pregnancies, 10 of which have already come to term with the birth of normal children, including one set of twins. Of the remainder, 2 are known to have oligozoospermia and 4 have been lost to follow-up although 2 of these were euspermic when last heard of. In spite of the encouraging results it is considered that there are no grounds for altering the present basis of vasectomy counselling in which the operation is described as likely to be irreversible."}
{"id": "PMID:465965", "title": "Oestrogen-induced renal carcinoma.", "content": "Oestrogen may be implicated as an aetiological factor in the development of some renal carcinomas in man. The development of a renal carcinoma in 2 patients who had been treated with low-dose oestrogen for periods of 3 and 3 1/2 years because of prostatic carcinoma is described. It is suggested that patients receiving prolonged oestrogen therapy should be evaluated for the presence of a renal carcinoma at regular intervals.", "contents": "Oestrogen-induced renal carcinoma. Oestrogen may be implicated as an aetiological factor in the development of some renal carcinomas in man. The development of a renal carcinoma in 2 patients who had been treated with low-dose oestrogen for periods of 3 and 3 1/2 years because of prostatic carcinoma is described. It is suggested that patients receiving prolonged oestrogen therapy should be evaluated for the presence of a renal carcinoma at regular intervals."}
{"id": "PMID:465966", "title": "Vesicoureteric reflux with urethral valves.", "content": "A retrospective review was undertaken of 66 boys with posterior urethral valves with regard to incidence and prognostic significance of vesicoureteric reflux. The results indicate that: Regardless of the age of the patient, the existence of bilateral reflux at the time of presentation implies a worse prognosis than does unilateral or no reflux. Reflux which does not stop spontaneously after the valves have been destroyed is generally associated with a functionless and often dysplastic kidney; surgical cure of such reflux is therefore pointless. Reflux stops spontaneously after valve ablation when the involved kidney possesses useful function; surgical cure of reflux is therefore unnecessary. In some cases, however, it remains possible that reflux may continue to damage the kidney during the interval between relief of the urethral obstruction and spontaneous cure of the reflux. Nevertheless, the grossly dilated refluxing ureter is poor surgical material and it is concluded that the best chance of preserving renal function in the majority of boys with urethral valves and reflux lies in endoscopic fulguration of the valves, controlling infection and awaiting spontaneous reflux cure.", "contents": "Vesicoureteric reflux with urethral valves. A retrospective review was undertaken of 66 boys with posterior urethral valves with regard to incidence and prognostic significance of vesicoureteric reflux. The results indicate that: Regardless of the age of the patient, the existence of bilateral reflux at the time of presentation implies a worse prognosis than does unilateral or no reflux. Reflux which does not stop spontaneously after the valves have been destroyed is generally associated with a functionless and often dysplastic kidney; surgical cure of such reflux is therefore pointless. Reflux stops spontaneously after valve ablation when the involved kidney possesses useful function; surgical cure of reflux is therefore unnecessary. In some cases, however, it remains possible that reflux may continue to damage the kidney during the interval between relief of the urethral obstruction and spontaneous cure of the reflux. Nevertheless, the grossly dilated refluxing ureter is poor surgical material and it is concluded that the best chance of preserving renal function in the majority of boys with urethral valves and reflux lies in endoscopic fulguration of the valves, controlling infection and awaiting spontaneous reflux cure."}
{"id": "PMID:465967", "title": "End-to-end ureteroileal anastomosis for ileal conduits.", "content": "In the \"Y\" anastomosis, both ureters are spatulated and joined together to form a single tube which is anastomosed to the proximal end of the ileum. This technique has several theoretical advantages which appear to be borne out in practice when the results of its use in 101 patients followed for up to 12 years are reviewed. Only 2 anastomotic leaks needed operative treatment and only 4 patients developed ureteroileal stenosis. Renal function remained unchanged or improved in 84% of the patients, and definite hydronephorsis or pyelonephritis developed in only 4% of the kidneys. These results show that this method of ureteroileal anastomosis is at least as good as, and possibly better than, other techniques.", "contents": "End-to-end ureteroileal anastomosis for ileal conduits. In the \"Y\" anastomosis, both ureters are spatulated and joined together to form a single tube which is anastomosed to the proximal end of the ileum. This technique has several theoretical advantages which appear to be borne out in practice when the results of its use in 101 patients followed for up to 12 years are reviewed. Only 2 anastomotic leaks needed operative treatment and only 4 patients developed ureteroileal stenosis. Renal function remained unchanged or improved in 84% of the patients, and definite hydronephorsis or pyelonephritis developed in only 4% of the kidneys. These results show that this method of ureteroileal anastomosis is at least as good as, and possibly better than, other techniques."}
{"id": "PMID:465968", "title": "Urinary incontinence in the female. The value of detrusor reflex activation procedures.", "content": "One hundred consecutive female patients with urinary incontinence were investigated with CO2 cystometry including detrusor reflex activation procedures such as postural change and ability to suppress self-induced detrusor contractions. Detrusor hyperreflexia was seen in 20 patients during bladder filling in the supine position, and in an additional 35 patients after detrusor reflex activation procedures. Four different types of detrusor hyperreflexia are described based on the cystometric findings. In 38 patients treated with parasympatholytics, 66% showed a good result independent of the type of detrusor hyperreflexia.", "contents": "Urinary incontinence in the female. The value of detrusor reflex activation procedures. One hundred consecutive female patients with urinary incontinence were investigated with CO2 cystometry including detrusor reflex activation procedures such as postural change and ability to suppress self-induced detrusor contractions. Detrusor hyperreflexia was seen in 20 patients during bladder filling in the supine position, and in an additional 35 patients after detrusor reflex activation procedures. Four different types of detrusor hyperreflexia are described based on the cystometric findings. In 38 patients treated with parasympatholytics, 66% showed a good result independent of the type of detrusor hyperreflexia."}
{"id": "PMID:465969", "title": "The effect on erection and orgasm of cystectomy, prostatectomy and vesiculectomy for cancer of the bladder: a clinical and electromyographic study.", "content": "Forty-three men who had been subjected to cystectomy and concomitant prostatectomy, vesiculectomy and urethrectomy were interviewed about their pre-operative and post-operative sexual activities at a mean of 3 (range 1 to 8) years after operation. Twenty-eight of the 38 men (74%) who had been sexually active continued to have some form of sexual activity, 21 of them achieving orgasm. Only 3 men had penile erection; 2 of them had been subjected to prostatectomy and 1 to prostatic resection. One of these men treated by prostatectomy had also had urethrectomy. Electromyographic registration from the striated external urethral sphincter, the bulbocavernosus muscle and the levator ani muscle showed normal duration of muscular contractions and length of interval between contractions after operation. The pattern of impulses during organs did not differ from that of normal men.", "contents": "The effect on erection and orgasm of cystectomy, prostatectomy and vesiculectomy for cancer of the bladder: a clinical and electromyographic study. Forty-three men who had been subjected to cystectomy and concomitant prostatectomy, vesiculectomy and urethrectomy were interviewed about their pre-operative and post-operative sexual activities at a mean of 3 (range 1 to 8) years after operation. Twenty-eight of the 38 men (74%) who had been sexually active continued to have some form of sexual activity, 21 of them achieving orgasm. Only 3 men had penile erection; 2 of them had been subjected to prostatectomy and 1 to prostatic resection. One of these men treated by prostatectomy had also had urethrectomy. Electromyographic registration from the striated external urethral sphincter, the bulbocavernosus muscle and the levator ani muscle showed normal duration of muscular contractions and length of interval between contractions after operation. The pattern of impulses during organs did not differ from that of normal men."}
{"id": "PMID:465970", "title": "Gelatin foam and isobutyl-2-cyanoacrylate in the treatment of life-threatening bladder haemorrhage by selective transcatheter embolisation of the internal iliac arteries.", "content": "Three patients with severe haemorrhage from inoperable bladder cancer were treated by selective transcatheter embolisation of both internal iliac arteries. The technical details, results and a rare complication are described. Both gelatin foam and isobutyl-2-cyanoacrylate (IBC) are suitable embolic materials, but we prefer IBC when possible in patients with tumour.", "contents": "Gelatin foam and isobutyl-2-cyanoacrylate in the treatment of life-threatening bladder haemorrhage by selective transcatheter embolisation of the internal iliac arteries. Three patients with severe haemorrhage from inoperable bladder cancer were treated by selective transcatheter embolisation of both internal iliac arteries. The technical details, results and a rare complication are described. Both gelatin foam and isobutyl-2-cyanoacrylate (IBC) are suitable embolic materials, but we prefer IBC when possible in patients with tumour."}
{"id": "PMID:465971", "title": "The assessment of prostatic obstruction from urodynamic measurements and from residual urine.", "content": "One hundred and seventeen males over the age of 55 were investigated for possible prostatic obstruction. About half of the cases in this series could have been objectively classified as unobstructed or obstructed from the maximum flow rate alone. In about two-thirds of the cases obstruction could be satisfactorily assessed from the maximum flow rate together with the detrusor pressure at maximum flow. It was not helpful to combine these 2 measurements into a single urethral resistance factor. In the remaining one-third of the cases, obstruction could be objectively assessed only from a plot of detrusor pressure against flow rate throughout micturition. In many of these cases both the pressure and the flow rate were low and the main peculiarity was that the contractile power of the bladder was weak. Residual urine is a sign of an abnormality of bladder function rather than the direct result of urethral obstruction.", "contents": "The assessment of prostatic obstruction from urodynamic measurements and from residual urine. One hundred and seventeen males over the age of 55 were investigated for possible prostatic obstruction. About half of the cases in this series could have been objectively classified as unobstructed or obstructed from the maximum flow rate alone. In about two-thirds of the cases obstruction could be satisfactorily assessed from the maximum flow rate together with the detrusor pressure at maximum flow. It was not helpful to combine these 2 measurements into a single urethral resistance factor. In the remaining one-third of the cases, obstruction could be objectively assessed only from a plot of detrusor pressure against flow rate throughout micturition. In many of these cases both the pressure and the flow rate were low and the main peculiarity was that the contractile power of the bladder was weak. Residual urine is a sign of an abnormality of bladder function rather than the direct result of urethral obstruction."}
{"id": "PMID:465972", "title": "Lateral curvature of the erect phallus without chordee.", "content": "Three cases of lateral curvature of the penis during erection are described. In no case was there evidence of chordee. A corrective operation for this condition is described.", "contents": "Lateral curvature of the erect phallus without chordee. Three cases of lateral curvature of the penis during erection are described. In no case was there evidence of chordee. A corrective operation for this condition is described."}
{"id": "PMID:465973", "title": "Primary malignant melanoma of the penis. Two cases and a review of the literature.", "content": "Two cases of primary malignant melanoma of the penis are reported and 35 cases from the world literature are reviewed. The tumour most commonly started in the glans penis and less frequently from the prepuce. The microscopic appearances were identical with those of tumours arising elsewhere in the skin. A comparison is made between the incidence of melanoma of the penis and melanoma occurring elsewhere on the body surface. Evaluation of the best treatment was difficult because the total number of cases was small and the methods of treatment and results so variable. It would seem that the prognosis of malignant melanoma of the penis is generally poor, although there are sufficient examples of long-term survival after total amputation in Stage I disease to suggest that this radical treatment is justified.", "contents": "Primary malignant melanoma of the penis. Two cases and a review of the literature. Two cases of primary malignant melanoma of the penis are reported and 35 cases from the world literature are reviewed. The tumour most commonly started in the glans penis and less frequently from the prepuce. The microscopic appearances were identical with those of tumours arising elsewhere in the skin. A comparison is made between the incidence of melanoma of the penis and melanoma occurring elsewhere on the body surface. Evaluation of the best treatment was difficult because the total number of cases was small and the methods of treatment and results so variable. It would seem that the prognosis of malignant melanoma of the penis is generally poor, although there are sufficient examples of long-term survival after total amputation in Stage I disease to suggest that this radical treatment is justified."}
{"id": "PMID:465974", "title": "Tumours of the testis in Jamaica.", "content": "Malignant tumours of the testis are reported to be rare in negroes. All cases reported in the records of the University Hospital of the West Indies and the Jamaica Cancer Registry over the period June 1958 to December 1976 were studied. There were 26 cases, giving a world standardised incidence of 0.40 per 100,000 per year. This incidence is higher than that seen in African negroes but lower than that of negroes in North America. The peak age incidence is a decade later than that commonly seen in high incidence countries.", "contents": "Tumours of the testis in Jamaica. Malignant tumours of the testis are reported to be rare in negroes. All cases reported in the records of the University Hospital of the West Indies and the Jamaica Cancer Registry over the period June 1958 to December 1976 were studied. There were 26 cases, giving a world standardised incidence of 0.40 per 100,000 per year. This incidence is higher than that seen in African negroes but lower than that of negroes in North America. The peak age incidence is a decade later than that commonly seen in high incidence countries."}
{"id": "PMID:465975", "title": "The pattern of spread and treatment of metastases in testicular seminoma.", "content": "The results of treatment of 199 patients referred to the Royal Marsden Hospital with testiclar seminoma from 1963 to 1975 are described. Of 190 previously untreated patients 16 (8.4%) have died of seminoma, 3 (1.5%) of teratoma and 9 (4.9%) of intercurrent disease. Of 121 Stage I patients the only death was due to a second testicular tumour. In orchiectomy and volume of abdominal node metastases. This was not, however, prognostically significant. Death from seminoma occurred in 6 out of 38 patients (15.8%) with nodal metastases less than or equal to 5 cm in diameter and 3 out of 16 patients (18.7%) with metastases greater than 5 cm in diameter; 2 of 8 Stage III patients died of seminoma. Thus radiation controlled nodal metastases in more than 80% of Stage I, II and III patients. In Stage IV patients and patients referred with disease relapse, 12 out of 16 patients died of seminoma. Chemotherapy experience is briefly reviewed. Cyclophosphamide as a single agent has produced a 50% response rate. There is no evidence to suggest a radiation-related increase in mortality from the group of long-term tumour-free survivors.", "contents": "The pattern of spread and treatment of metastases in testicular seminoma. The results of treatment of 199 patients referred to the Royal Marsden Hospital with testiclar seminoma from 1963 to 1975 are described. Of 190 previously untreated patients 16 (8.4%) have died of seminoma, 3 (1.5%) of teratoma and 9 (4.9%) of intercurrent disease. Of 121 Stage I patients the only death was due to a second testicular tumour. In orchiectomy and volume of abdominal node metastases. This was not, however, prognostically significant. Death from seminoma occurred in 6 out of 38 patients (15.8%) with nodal metastases less than or equal to 5 cm in diameter and 3 out of 16 patients (18.7%) with metastases greater than 5 cm in diameter; 2 of 8 Stage III patients died of seminoma. Thus radiation controlled nodal metastases in more than 80% of Stage I, II and III patients. In Stage IV patients and patients referred with disease relapse, 12 out of 16 patients died of seminoma. Chemotherapy experience is briefly reviewed. Cyclophosphamide as a single agent has produced a 50% response rate. There is no evidence to suggest a radiation-related increase in mortality from the group of long-term tumour-free survivors."}
{"id": "PMID:465977", "title": "Genetic associations of transitional cell carcinoma.", "content": "A series of 101 cases of transitional cell carcinoma (TCC) was contrasted with a control series for several genetic parameters. Three genetic associations were demonstrated with the TCC patients having A gene frequencies, HLA B5 and HLA CW4 genes all higher than might be expected by chance. A classification of the natural history of the disease is used to show that the HLA frequencies vary with the more or the less severe forms of the disease.", "contents": "Genetic associations of transitional cell carcinoma. A series of 101 cases of transitional cell carcinoma (TCC) was contrasted with a control series for several genetic parameters. Three genetic associations were demonstrated with the TCC patients having A gene frequencies, HLA B5 and HLA CW4 genes all higher than might be expected by chance. A classification of the natural history of the disease is used to show that the HLA frequencies vary with the more or the less severe forms of the disease."}
{"id": "PMID:465978", "title": "The value of large volume selective arteriophlebography of the renal veins in the pre-operative assessment of renal carcinoma.", "content": "The accuracy of large volume selective arteriophlebography in demonstrating the main renal veins in 28 patients with renal carcinoma is presented. The diagnosis was shown to be correct at operation in 96% of examinations. It is suggested that the technique is valuable and safe and should form part of the routine pre-operative assessment of patients with renal carcinoma.", "contents": "The value of large volume selective arteriophlebography of the renal veins in the pre-operative assessment of renal carcinoma. The accuracy of large volume selective arteriophlebography in demonstrating the main renal veins in 28 patients with renal carcinoma is presented. The diagnosis was shown to be correct at operation in 96% of examinations. It is suggested that the technique is valuable and safe and should form part of the routine pre-operative assessment of patients with renal carcinoma."}
{"id": "PMID:465979", "title": "Familial renal hypouricaemia: two additional cases with uric acid lithiasis.", "content": "Two families are reported affected with hereditary renal hypouricaemia associated with uric acid lithiasis. The propositi of both families were found also to have hyperabsorptive hypercalciuria. Based on the study of the effect of pyrazinamide and probenecid on uric acid excretion in both propositi, it is suggested that the defect in uric acid reabsorption is most probably at the pre-secretory site.", "contents": "Familial renal hypouricaemia: two additional cases with uric acid lithiasis. Two families are reported affected with hereditary renal hypouricaemia associated with uric acid lithiasis. The propositi of both families were found also to have hyperabsorptive hypercalciuria. Based on the study of the effect of pyrazinamide and probenecid on uric acid excretion in both propositi, it is suggested that the defect in uric acid reabsorption is most probably at the pre-secretory site."}
{"id": "PMID:465980", "title": "False-negative saralasin responses in renovascular hypertension.", "content": "Blood pressure responses to the infusion of saralasin and plasma renin levels were measured in 31 hypertensive patients following preparation with frusemide. Five patients had unilateral renal artery stenosis, with renal vein ratios lateralising strongly to the affected side. Saralasin produced depressor responses in 3 of these but failed to evoke significant effects in the other 2, despite the fact that in both cases hypertension was subsequently alleviated by renal bypass surgery. A further period of more severe sodium depletion increased plasma renin levels and the depressor effect of saralasin, but did not help to differentiate renal artery stenosis from other forms of hypertension.", "contents": "False-negative saralasin responses in renovascular hypertension. Blood pressure responses to the infusion of saralasin and plasma renin levels were measured in 31 hypertensive patients following preparation with frusemide. Five patients had unilateral renal artery stenosis, with renal vein ratios lateralising strongly to the affected side. Saralasin produced depressor responses in 3 of these but failed to evoke significant effects in the other 2, despite the fact that in both cases hypertension was subsequently alleviated by renal bypass surgery. A further period of more severe sodium depletion increased plasma renin levels and the depressor effect of saralasin, but did not help to differentiate renal artery stenosis from other forms of hypertension."}
{"id": "PMID:465981", "title": "An ultrastructural study on the effects of warm ischaemia on the inosine-protected kidney.", "content": "Unperfused, saline-perfused and inosine-perfused rat kidneys were subjected to various times of warm ischaemia with and without resumption of blood flow. Tissues were examined with a transmission electron microscope. Inosine has a protective effect on the epithelial cells of the proximal convoluted tubules, where microvilli are less damaged by ischaemia than those of the proximal convoluted tubular cells of the control animals. Three is also a much higher ratio of autophagic vacuoles to lysosomes present in cells of inosine-treated kidneys, reflecting their ability to sequester and digest organelles damaged during ischaemia and thus protect themselves from degradation products. A major effect of inosine seems to be the preservation of the organelles and rush border of the cells of the proximal tubule during warm ischaemia.", "contents": "An ultrastructural study on the effects of warm ischaemia on the inosine-protected kidney. Unperfused, saline-perfused and inosine-perfused rat kidneys were subjected to various times of warm ischaemia with and without resumption of blood flow. Tissues were examined with a transmission electron microscope. Inosine has a protective effect on the epithelial cells of the proximal convoluted tubules, where microvilli are less damaged by ischaemia than those of the proximal convoluted tubular cells of the control animals. Three is also a much higher ratio of autophagic vacuoles to lysosomes present in cells of inosine-treated kidneys, reflecting their ability to sequester and digest organelles damaged during ischaemia and thus protect themselves from degradation products. A major effect of inosine seems to be the preservation of the organelles and rush border of the cells of the proximal tubule during warm ischaemia."}
{"id": "PMID:465982", "title": "Ectopic kidneys with and without fusion.", "content": "Sixty-one patients with renal ectopia have been reviewed. Most presented with a palpable mass, abdominal pain or microscopic haematuria. Thirteen patients were treated surgically, usually by nephrectomy; 17 required no treatment and 31 were treated symptomatically.", "contents": "Ectopic kidneys with and without fusion. Sixty-one patients with renal ectopia have been reviewed. Most presented with a palpable mass, abdominal pain or microscopic haematuria. Thirteen patients were treated surgically, usually by nephrectomy; 17 required no treatment and 31 were treated symptomatically."}
{"id": "PMID:465983", "title": "Effects of therapy with bendroflumethiazide in patients with recurrent renal calcium stones.", "content": "Forty-four patients with recurrent formation of calcium-containing renal stones were treated with bendroflumethiazide for at least 2 years. Prior to treatment each patient had formed, on average, one stone per year for 8 years; during treatment only 4 patients formed new stones. A reduction in urinary calcium excretion was seen in almost all patients irrespective of their initial urinary calcium level. The apparent clinical benefit was not related to pre-treatment urinary electrolyte levels. Side effects were slight: one patient developed symptomatic hyperuricaemia and in one case sustained hypercalcaemia was found. Long-term treatment with thiazides appears to be a safe and effective method for the prevention of recurrent calcium stones.", "contents": "Effects of therapy with bendroflumethiazide in patients with recurrent renal calcium stones. Forty-four patients with recurrent formation of calcium-containing renal stones were treated with bendroflumethiazide for at least 2 years. Prior to treatment each patient had formed, on average, one stone per year for 8 years; during treatment only 4 patients formed new stones. A reduction in urinary calcium excretion was seen in almost all patients irrespective of their initial urinary calcium level. The apparent clinical benefit was not related to pre-treatment urinary electrolyte levels. Side effects were slight: one patient developed symptomatic hyperuricaemia and in one case sustained hypercalcaemia was found. Long-term treatment with thiazides appears to be a safe and effective method for the prevention of recurrent calcium stones."}
{"id": "PMID:465984", "title": "Dietary treatment of idiopathic hypercalciuria.", "content": "Eighty-eight urinary tract stone formers (74 men) with idiopathic hypercalciuria were treated by dietary calcium restriction alone. Short-term control of hypercalciuria was achieved in only 27 patients and all but 12 eventually escaped control. Failure of control was twice as likely in patients with severe hypercalciuria. Almost all patients lived in a hard water area. In such areas at least, attempts to control hypercalciuria by diet alone are likely to fail and early introduction of additional treatment is advisable. In most severe hypercalciurics, such treatment should be introduced from the start.", "contents": "Dietary treatment of idiopathic hypercalciuria. Eighty-eight urinary tract stone formers (74 men) with idiopathic hypercalciuria were treated by dietary calcium restriction alone. Short-term control of hypercalciuria was achieved in only 27 patients and all but 12 eventually escaped control. Failure of control was twice as likely in patients with severe hypercalciuria. Almost all patients lived in a hard water area. In such areas at least, attempts to control hypercalciuria by diet alone are likely to fail and early introduction of additional treatment is advisable. In most severe hypercalciurics, such treatment should be introduced from the start."}
{"id": "PMID:465985", "title": "Transvaginal milking of lower ureteric stones into the bladder.", "content": "In a selected group of adult female patients, stones in the lower ureter were transvaginally milked into the bladder. The transvaginal milking of lower ureteric calculi into the bladder is easy and safe and is recommended in the poor surgical risk patient.", "contents": "Transvaginal milking of lower ureteric stones into the bladder. In a selected group of adult female patients, stones in the lower ureter were transvaginally milked into the bladder. The transvaginal milking of lower ureteric calculi into the bladder is easy and safe and is recommended in the poor surgical risk patient."}
{"id": "PMID:465986", "title": "A trial of prophylactic radiotherapy for non-infiltrating bladder tumours.", "content": "In series of 56 cases of non-invasive bladder tumours there was a 61.8% recurrence rate after endoscopic treatment. In 29 cases a trial of prophylactic, very low dose (750 rad) radiotherapy was given; this was administered within a few days of the primary treatment. Twenty-seven cases received no radiotherapy. Over follow-up periods of 2 to 5 years there were recurrences in 12 of the irradiated group compared with 11 out of the 27 controls.", "contents": "A trial of prophylactic radiotherapy for non-infiltrating bladder tumours. In series of 56 cases of non-invasive bladder tumours there was a 61.8% recurrence rate after endoscopic treatment. In 29 cases a trial of prophylactic, very low dose (750 rad) radiotherapy was given; this was administered within a few days of the primary treatment. Twenty-seven cases received no radiotherapy. Over follow-up periods of 2 to 5 years there were recurrences in 12 of the irradiated group compared with 11 out of the 27 controls."}
{"id": "PMID:465987", "title": "Detrusor instability following surgery for genuine stress incontinence.", "content": "Nnety-two female patients with genuine stress incontinence, and with otherwise normal bladder function, were treated by a colposuspension operation. Pre-operative and post-operative clinical and urodynamic assessments were performed on all patients. Following surgery 17 of them were found to have detrusor instability.", "contents": "Detrusor instability following surgery for genuine stress incontinence. Nnety-two female patients with genuine stress incontinence, and with otherwise normal bladder function, were treated by a colposuspension operation. Pre-operative and post-operative clinical and urodynamic assessments were performed on all patients. Following surgery 17 of them were found to have detrusor instability."}
{"id": "PMID:465988", "title": "Vesicovaginal fistulae.", "content": "The aetiology, methods of repair and results are described in 616 patients with vesicovaginal fistulae (26 also had rectovaginal fistulae). The cure rate was 81%.", "contents": "Vesicovaginal fistulae. The aetiology, methods of repair and results are described in 616 patients with vesicovaginal fistulae (26 also had rectovaginal fistulae). The cure rate was 81%."}
{"id": "PMID:465989", "title": "Complications of antifibrinolysis therapy after prostatectomy.", "content": "As part of a trial to compare the effectiveness of various agents which have been claimed to reduce blood loss after prostatectomy, tranexamic acid (AMCA) was given pre-operatively and post-operatively to 6 patients having routine transurethral resections. Three of these developed indissoluble intravesical blood clots which persisted until they were evacuated surgically 5 to 17 days after cessation of AMCA therapy. AMCA cannot be recommended for routine use after prostatectomy.", "contents": "Complications of antifibrinolysis therapy after prostatectomy. As part of a trial to compare the effectiveness of various agents which have been claimed to reduce blood loss after prostatectomy, tranexamic acid (AMCA) was given pre-operatively and post-operatively to 6 patients having routine transurethral resections. Three of these developed indissoluble intravesical blood clots which persisted until they were evacuated surgically 5 to 17 days after cessation of AMCA therapy. AMCA cannot be recommended for routine use after prostatectomy."}
{"id": "PMID:465990", "title": "Acid phosphatase after examination of the prostate.", "content": "We report the effect of rectal examination of the prostate and transurethral resection of the prostate (TURP) on the serum level of tartrate labile acid phosphatase (TLAP) in 18 normal cases, 31 with benign enlargement of the prostate and 20 with carcinoma. Rectal examination had no effect on TLAP in normal patients, those with benign enlargement and those with carcinoma, either after 5 min or the following morning. TURP for both benign and malignant disease raised the TLAP transiently with a rapid return to normal. We believe that there is no justification for postponing estimation of TLAP following rectal examination.", "contents": "Acid phosphatase after examination of the prostate. We report the effect of rectal examination of the prostate and transurethral resection of the prostate (TURP) on the serum level of tartrate labile acid phosphatase (TLAP) in 18 normal cases, 31 with benign enlargement of the prostate and 20 with carcinoma. Rectal examination had no effect on TLAP in normal patients, those with benign enlargement and those with carcinoma, either after 5 min or the following morning. TURP for both benign and malignant disease raised the TLAP transiently with a rapid return to normal. We believe that there is no justification for postponing estimation of TLAP following rectal examination."}
{"id": "PMID:465991", "title": "The early results of treatment of stricture of the male urethra using the Sachse optical urethrotome.", "content": "Thirty-nine male patients with urethral stricture have undergone urethrotomy using the Sachse optical urethrotome. Post-operatively a urethral catheter was used for 24 to 72 h. The technique is simple, the stay in hospital is short and complications are uncommon. Eighty-two per cent of the patients are now symptom-free and further 13% are sufficiently improved to be able to void satisfactorily at follow-up varying from 4 months to 2 1/2 years (mean 12 months). The 2 patients in whom unsatisfactory results were obtained had long strictures. We feel that direct vision urethrotomy should be considered as a treatment for urethral stricture.", "contents": "The early results of treatment of stricture of the male urethra using the Sachse optical urethrotome. Thirty-nine male patients with urethral stricture have undergone urethrotomy using the Sachse optical urethrotome. Post-operatively a urethral catheter was used for 24 to 72 h. The technique is simple, the stay in hospital is short and complications are uncommon. Eighty-two per cent of the patients are now symptom-free and further 13% are sufficiently improved to be able to void satisfactorily at follow-up varying from 4 months to 2 1/2 years (mean 12 months). The 2 patients in whom unsatisfactory results were obtained had long strictures. We feel that direct vision urethrotomy should be considered as a treatment for urethral stricture."}
{"id": "PMID:465992", "title": "Balanitis xerotica obliterans.", "content": "Twenty patients with histologically proven balantis xerotica obliterans have undergone different forms of treatment; their progress has been reviewed and discussed. Balanitis xerotica obliterans involving only the foreskin was best treated by circumcision. The patients in whom meatal stenosis was present responded well to regular meatal dilatation, meatotomy followed by regular dilatation and, in advanced cases, to meatoplasty. At present there is no evidence to indicate that associated urethral strictures, proximal to the fossa navicularis, are due to balanitis xerotica obliterans.", "contents": "Balanitis xerotica obliterans. Twenty patients with histologically proven balantis xerotica obliterans have undergone different forms of treatment; their progress has been reviewed and discussed. Balanitis xerotica obliterans involving only the foreskin was best treated by circumcision. The patients in whom meatal stenosis was present responded well to regular meatal dilatation, meatotomy followed by regular dilatation and, in advanced cases, to meatoplasty. At present there is no evidence to indicate that associated urethral strictures, proximal to the fossa navicularis, are due to balanitis xerotica obliterans."}
{"id": "PMID:465996", "title": "Genitourinary tuberculosis: a 7-year review.", "content": "Since 1970 short course regimes of chemotherapy have been adopted for the treatment of 87 patients suffering from genitourinary tuberculosis. The response to chemotherapy, the effect of surgery, the importance of hypertension and the relapse rate have been studied. It is concluded that all cases of genitourinary tuberculosis will respond to a short intensive course of chemotherapy, which need not be given for longer than 6 months.", "contents": "Genitourinary tuberculosis: a 7-year review. Since 1970 short course regimes of chemotherapy have been adopted for the treatment of 87 patients suffering from genitourinary tuberculosis. The response to chemotherapy, the effect of surgery, the importance of hypertension and the relapse rate have been studied. It is concluded that all cases of genitourinary tuberculosis will respond to a short intensive course of chemotherapy, which need not be given for longer than 6 months."}
{"id": "PMID:465997", "title": "Nephroplasty in the management of hydronephrosis.", "content": "Seventeen patients with hydronephrosis were treated by nephroplasty and pyeloplasty. This combination proved to be a simple and effective way of reducing intrarenal dilatation and encouraging dependent drainage from the lower pole calices.", "contents": "Nephroplasty in the management of hydronephrosis. Seventeen patients with hydronephrosis were treated by nephroplasty and pyeloplasty. This combination proved to be a simple and effective way of reducing intrarenal dilatation and encouraging dependent drainage from the lower pole calices."}
{"id": "PMID:465998", "title": "Family history of renal stones in a population study of stone-formers and health subjects.", "content": "Information on the family history of renal stone disease was obtained from over 2500 middle-aged men in a general health survey. A family history of stones was significantly more common among the stone-formers than amoung the controls, largely due to an increased frequency of stones among the fathers and brothers of the propositi. This study thus supports previous suggestions that genetic factors play a part in the predisposition to stone formation. An increased tendency was also noted, however, among the wives of those whose relatives had stones, which suggests that environmental factors are involved. Subjects with a family history of renal stones did not exhibit any particular characteristics in their laboratory profiles, but they appeared to be more prone to develop early and frequent recurrences.", "contents": "Family history of renal stones in a population study of stone-formers and health subjects. Information on the family history of renal stone disease was obtained from over 2500 middle-aged men in a general health survey. A family history of stones was significantly more common among the stone-formers than amoung the controls, largely due to an increased frequency of stones among the fathers and brothers of the propositi. This study thus supports previous suggestions that genetic factors play a part in the predisposition to stone formation. An increased tendency was also noted, however, among the wives of those whose relatives had stones, which suggests that environmental factors are involved. Subjects with a family history of renal stones did not exhibit any particular characteristics in their laboratory profiles, but they appeared to be more prone to develop early and frequent recurrences."}
{"id": "PMID:465999", "title": "The modified lumbotomy versus the oblique loin incision for renal surgery.", "content": "The time to full mobility and total analgesic requirements have been assessed in 40 patients undergoing renal surgery by the oblique loin incision or the posterior lumbotomy approach. By these criteria the lumbotomy group fared better than patients who had oblique incisions. We consider lumbotomy to be a valuable incision which should be in the repertoire of every urologist.", "contents": "The modified lumbotomy versus the oblique loin incision for renal surgery. The time to full mobility and total analgesic requirements have been assessed in 40 patients undergoing renal surgery by the oblique loin incision or the posterior lumbotomy approach. By these criteria the lumbotomy group fared better than patients who had oblique incisions. We consider lumbotomy to be a valuable incision which should be in the repertoire of every urologist."}
{"id": "PMID:466000", "title": "Benign tumours developing at the site of ureterosigmoidostomy.", "content": "Two cases of benign tumours complicating ureterosigmoidostomy are described. The latent period between diversion and the development of tumour is long and careful follow-up of patients with ureterosigmoidostomy is recommended. It would seem highly probable that adenomatous polyps developing at the site of anastomosis may, if undetected, undergo malignant change to adenocarcinoma. All of the tumours recorded following ureterosigmoid diversion have been associated with a nipple type ureterocolic anastomosis and mucosa-to-mucosa anastomosis may possibly prevent this complication.", "contents": "Benign tumours developing at the site of ureterosigmoidostomy. Two cases of benign tumours complicating ureterosigmoidostomy are described. The latent period between diversion and the development of tumour is long and careful follow-up of patients with ureterosigmoidostomy is recommended. It would seem highly probable that adenomatous polyps developing at the site of anastomosis may, if undetected, undergo malignant change to adenocarcinoma. All of the tumours recorded following ureterosigmoid diversion have been associated with a nipple type ureterocolic anastomosis and mucosa-to-mucosa anastomosis may possibly prevent this complication."}
{"id": "PMID:466001", "title": "Bladder augmentation--a long-term review.", "content": "Fifty-nine patients who had undergone augmentation cystoplasty were studied over a period of 18 years. The indications for the operation were a tuberculous contracted bladder in 51, interstitial cystitis in 7 and carcinoma in 1. The ileum was used in one patient, the colon in 16 and the caecum in 42. The results did not seem to be influenced by the segment of bowel and the long-term results of using the colon as bladder substitute were similar to those achieved by using the caecum. An excellent result has been achieved in 78% of the 49 surviving patients. Operative mortality was 5.1% (3 patients). Contraindications include progressive severe renal failure, enuresis and a history of previous psychiatric disturbance.", "contents": "Bladder augmentation--a long-term review. Fifty-nine patients who had undergone augmentation cystoplasty were studied over a period of 18 years. The indications for the operation were a tuberculous contracted bladder in 51, interstitial cystitis in 7 and carcinoma in 1. The ileum was used in one patient, the colon in 16 and the caecum in 42. The results did not seem to be influenced by the segment of bowel and the long-term results of using the colon as bladder substitute were similar to those achieved by using the caecum. An excellent result has been achieved in 78% of the 49 surviving patients. Operative mortality was 5.1% (3 patients). Contraindications include progressive severe renal failure, enuresis and a history of previous psychiatric disturbance."}
{"id": "PMID:466002", "title": "\"Undiversion\" with colocystoplasty for neurogenic bladder. A report of 2 cases.", "content": "The encouraging success of partial replacement of the bladder for interstitial cystitis and for contracted bladder due to tuberculosis, and the relative success of total bladder replacement for carcinoma, prompted an attempt to reconstruct a bladder in 2 patients with neuropathic bladders, who had problems with their ileal conduit and, thus, \"undivert\" them. Bladder reconstruction in these patients was performed using a modified technique of colocystoplasty. One of the patients has had 2 normal pregnancies since colocystoplasty.", "contents": "\"Undiversion\" with colocystoplasty for neurogenic bladder. A report of 2 cases. The encouraging success of partial replacement of the bladder for interstitial cystitis and for contracted bladder due to tuberculosis, and the relative success of total bladder replacement for carcinoma, prompted an attempt to reconstruct a bladder in 2 patients with neuropathic bladders, who had problems with their ileal conduit and, thus, \"undivert\" them. Bladder reconstruction in these patients was performed using a modified technique of colocystoplasty. One of the patients has had 2 normal pregnancies since colocystoplasty."}
{"id": "PMID:466003", "title": "Transection of the bladder as a method of treatment in adult enuresis nocturna.", "content": "Ten adults with enuresis were treated by bladder transection. Urodynamic assessment was carried out before and after the operation. No significant urodynamic changes were recorded following transection. However, good results were obtained in 5 cases and improvement in 2.", "contents": "Transection of the bladder as a method of treatment in adult enuresis nocturna. Ten adults with enuresis were treated by bladder transection. Urodynamic assessment was carried out before and after the operation. No significant urodynamic changes were recorded following transection. However, good results were obtained in 5 cases and improvement in 2."}
{"id": "PMID:466004", "title": "Acute phase reactant proteins in the clinical management of carcinoma of the bladder.", "content": "The levels of a number of acute phase reactant proteins have been measured in 250 patients with carcinoma of the bladder in all stages. Three of them, acid glycoprotein, antichymotrypsin, and C-reactive protein, frequently gave abnormal results in advanced disease. The levels of these 3 proteins in patients who were clinically tumour-free were not distinguishable from those from an age-matched control group. The levels in patients with T1 and T2 bladder tumours were sometimes elevated. In advanced disease, figures were commonly above the normal range.", "contents": "Acute phase reactant proteins in the clinical management of carcinoma of the bladder. The levels of a number of acute phase reactant proteins have been measured in 250 patients with carcinoma of the bladder in all stages. Three of them, acid glycoprotein, antichymotrypsin, and C-reactive protein, frequently gave abnormal results in advanced disease. The levels of these 3 proteins in patients who were clinically tumour-free were not distinguishable from those from an age-matched control group. The levels in patients with T1 and T2 bladder tumours were sometimes elevated. In advanced disease, figures were commonly above the normal range."}
{"id": "PMID:466005", "title": "Weight estimation of benign prostatic adenoma with urethral pressure profile.", "content": "An estimation of the weight of the benign prostatic adenoma by quantitative analysis of the urethral pressure profile has been correlated with the surgically removed specimen in 23 patients. It has been found that the length of the continence zone cane be used as an index of prostatic weight. After operation the length of the continence zone fell into the normal range of the age matched controls.", "contents": "Weight estimation of benign prostatic adenoma with urethral pressure profile. An estimation of the weight of the benign prostatic adenoma by quantitative analysis of the urethral pressure profile has been correlated with the surgically removed specimen in 23 patients. It has been found that the length of the continence zone cane be used as an index of prostatic weight. After operation the length of the continence zone fell into the normal range of the age matched controls."}
{"id": "PMID:466006", "title": "The effect of prostatectomy on urodynamic parameters.", "content": "Sixty per cent of men subjected to prostatectomy had unstable bladders. It was found that this was related to the presence of either an indwelling catheter or obstruction, but had little influence on the result of prostatectomy. The functional urethral pressure profile length did not correlate with the size of the prostate gland. Shortening of the prostatic plateau was always found, but the extent to which the plateau was reduced did not correlate with urine flow rates.", "contents": "The effect of prostatectomy on urodynamic parameters. Sixty per cent of men subjected to prostatectomy had unstable bladders. It was found that this was related to the presence of either an indwelling catheter or obstruction, but had little influence on the result of prostatectomy. The functional urethral pressure profile length did not correlate with the size of the prostate gland. Shortening of the prostatic plateau was always found, but the extent to which the plateau was reduced did not correlate with urine flow rates."}
{"id": "PMID:466007", "title": "Human prostatic lymphoscintigraphy. A preliminary report.", "content": "A simple method for demonstrating prostatic lymphatic drainage by transrectal injection of 99mTc labelled antimony sulphide colloid into the prostatic capsule has been developed. This technique may be readily applied in clinical practice with obvious potential in the assessment and follow-up of patients with carcinoma of the prostate.", "contents": "Human prostatic lymphoscintigraphy. A preliminary report. A simple method for demonstrating prostatic lymphatic drainage by transrectal injection of 99mTc labelled antimony sulphide colloid into the prostatic capsule has been developed. This technique may be readily applied in clinical practice with obvious potential in the assessment and follow-up of patients with carcinoma of the prostate."}
{"id": "PMID:466008", "title": "Fracture of the penis with laceration of the urethra.", "content": "Two patients are described with severe injury to the penis sustained during sexual intercourse. The literature is reviewed. A conservative approach to management with suprapubic urinary diversion led to acceptable results.", "contents": "Fracture of the penis with laceration of the urethra. Two patients are described with severe injury to the penis sustained during sexual intercourse. The literature is reviewed. A conservative approach to management with suprapubic urinary diversion led to acceptable results."}
{"id": "PMID:466009", "title": "Prophylactic vasectomy.", "content": "A prospective study of the value of vasectomy with prostatectomy is presented. Vasectomy significantly reduced the incidence of epididymitis after prostatectomy in the presence of pre-operative urinary tract infection and prolonged indwelling urethral catheterisation.", "contents": "Prophylactic vasectomy. A prospective study of the value of vasectomy with prostatectomy is presented. Vasectomy significantly reduced the incidence of epididymitis after prostatectomy in the presence of pre-operative urinary tract infection and prolonged indwelling urethral catheterisation."}
{"id": "PMID:466010", "title": "Fournier's gangrene.", "content": "Twenty-five cases of gangrene of the scrotum and penis have been analysed. The anterior scrotum or the whole scrotum were the areas most frequently involved. The results of seminal analyses showed marked oligozoospermia during the infective period. Haemolytic streptococcus was the most common organism. The aetiology of the disease is reviewed and \"infective gangrene of the scrotum and penis\" is considered to be a more suitable diagnostic label than Fournier's gangrene.", "contents": "Fournier's gangrene. Twenty-five cases of gangrene of the scrotum and penis have been analysed. The anterior scrotum or the whole scrotum were the areas most frequently involved. The results of seminal analyses showed marked oligozoospermia during the infective period. Haemolytic streptococcus was the most common organism. The aetiology of the disease is reviewed and \"infective gangrene of the scrotum and penis\" is considered to be a more suitable diagnostic label than Fournier's gangrene."}
{"id": "PMID:466012", "title": "Giant hydronephrosis.", "content": "A case of congenital, unilateral giant hydronephrosis containing 42 1/2 litres of urine in an adult male is reported. Diagnosis and management are discussed. Because of the possible effects of sudden abdominal decompression, a plea is made for a two-stage procedure. The hydronephrosis is first decompressed slowly by drainage and nephrectomy is then performed after the patient's cardiorespiratory and alimentary systems have been stabilised.", "contents": "Giant hydronephrosis. A case of congenital, unilateral giant hydronephrosis containing 42 1/2 litres of urine in an adult male is reported. Diagnosis and management are discussed. Because of the possible effects of sudden abdominal decompression, a plea is made for a two-stage procedure. The hydronephrosis is first decompressed slowly by drainage and nephrectomy is then performed after the patient's cardiorespiratory and alimentary systems have been stabilised."}
{"id": "PMID:466017", "title": "A new look at the arterial supply of the bile duct in man and its surgical implications.", "content": "The blood supply of the human bile duct has been re-evaluated using high resolution resin casts prepared from 24 fresh human cadavers. The refined technique used yielded casts of all vessels, including capillaries, and produced a clear picture of the blood supply of the human bile duct for the first time. The arterial supply of the supraduodenal duct was shown to be axial, with the main vessels, which have been named the 3 o'clock and 9 o'clock arteries, running along the lateral borders. The retroportal artery, which has not been described by previous workers, was present in all complete casts and was a major source of the axial blood supply to the supraduodenal duct in 32 per cent of them. The major importance of this new knowledge of bile duct blood supply may well lie in the understanding of the aetiology of postoperative bile duct strictures and in their prevention. An explanation is proposed for the long strictures sometimes seen after minimal surgical trauma to the bile duct, based on damage to the small vessels supplying the duct; guidelines to prevent such damage are presented. Ischaemia of the bile duct may also explain some of the biliary problems that have followed human liver transplantation and other procedures involving biliary anastomosis, such as Whipple's operation.", "contents": "A new look at the arterial supply of the bile duct in man and its surgical implications. The blood supply of the human bile duct has been re-evaluated using high resolution resin casts prepared from 24 fresh human cadavers. The refined technique used yielded casts of all vessels, including capillaries, and produced a clear picture of the blood supply of the human bile duct for the first time. The arterial supply of the supraduodenal duct was shown to be axial, with the main vessels, which have been named the 3 o'clock and 9 o'clock arteries, running along the lateral borders. The retroportal artery, which has not been described by previous workers, was present in all complete casts and was a major source of the axial blood supply to the supraduodenal duct in 32 per cent of them. The major importance of this new knowledge of bile duct blood supply may well lie in the understanding of the aetiology of postoperative bile duct strictures and in their prevention. An explanation is proposed for the long strictures sometimes seen after minimal surgical trauma to the bile duct, based on damage to the small vessels supplying the duct; guidelines to prevent such damage are presented. Ischaemia of the bile duct may also explain some of the biliary problems that have followed human liver transplantation and other procedures involving biliary anastomosis, such as Whipple's operation."}
{"id": "PMID:466018", "title": "Exploration of the common bile duct: a comparative study.", "content": "The results of a retrospective comparative study of 122 patients who underwent explorations of the common bile duct in a district general hospital over a 5-year period are presented. Seventy-eight patients had a supraduodenal choledochotomy, 32 had a transduodenal spincterotomy and 12 had a combination of both procedures. Particular attention was paid to postoperative complications in relation to the grade of the surgeon performing the operation and also to the age of the patient. A total of 78 (64 per cent) patients had definite stones in the common bile duct. Of these patients, 7 (9 per cent) were subsequently shown to have retained stones, all having been operated on by registrars. Registrars also did more negative explorations than consultants. Nine patients (7.4 per cent) died, all but 1 of them being over the age of 70 and 4 of them having had a combined procedure. We conclude that transduodenal exploration should be avoided in patients over 70, particularly when added to a supraduodenal exploration.", "contents": "Exploration of the common bile duct: a comparative study. The results of a retrospective comparative study of 122 patients who underwent explorations of the common bile duct in a district general hospital over a 5-year period are presented. Seventy-eight patients had a supraduodenal choledochotomy, 32 had a transduodenal spincterotomy and 12 had a combination of both procedures. Particular attention was paid to postoperative complications in relation to the grade of the surgeon performing the operation and also to the age of the patient. A total of 78 (64 per cent) patients had definite stones in the common bile duct. Of these patients, 7 (9 per cent) were subsequently shown to have retained stones, all having been operated on by registrars. Registrars also did more negative explorations than consultants. Nine patients (7.4 per cent) died, all but 1 of them being over the age of 70 and 4 of them having had a combined procedure. We conclude that transduodenal exploration should be avoided in patients over 70, particularly when added to a supraduodenal exploration."}
{"id": "PMID:466019", "title": "Renal function and other factors in obstructive jaundice.", "content": "Renal function and other factors that possibly affect the outcome of operation were measured in 24 patients with obstructive jaundice and in 15 non-jaundiced controls. The preoperative features that were associated with a poor postoperative recovery from obstructive jaundice were a raised serum fibrinogen/fibrin degradation product concentration, infection, hypoalbuminaemia and a low glomerular filtration rate. Preoperative serum fibrinogen/fibrin degradation product concentrations were raised in 4 of the 6 jaundiced patients who died after surgery but in none of the controls, in whom there was no mortality. In the jaundiced patients there was a greater incidence of postoperative renal impairment than in the controls. All patients were given mannitol during operation. Further mannitol was required after surgery in 13 of the 24 jaundiced patients in order to maintain urine flow rate despite adequate intravenous fluids being given. In contrast, only 1 of the 15 control patients required post operative mannitol. It is emphasized that repeated doses of mannitol can lead to a profound natriuresis and adequate intravenous saline should be given.", "contents": "Renal function and other factors in obstructive jaundice. Renal function and other factors that possibly affect the outcome of operation were measured in 24 patients with obstructive jaundice and in 15 non-jaundiced controls. The preoperative features that were associated with a poor postoperative recovery from obstructive jaundice were a raised serum fibrinogen/fibrin degradation product concentration, infection, hypoalbuminaemia and a low glomerular filtration rate. Preoperative serum fibrinogen/fibrin degradation product concentrations were raised in 4 of the 6 jaundiced patients who died after surgery but in none of the controls, in whom there was no mortality. In the jaundiced patients there was a greater incidence of postoperative renal impairment than in the controls. All patients were given mannitol during operation. Further mannitol was required after surgery in 13 of the 24 jaundiced patients in order to maintain urine flow rate despite adequate intravenous fluids being given. In contrast, only 1 of the 15 control patients required post operative mannitol. It is emphasized that repeated doses of mannitol can lead to a profound natriuresis and adequate intravenous saline should be given."}
{"id": "PMID:466020", "title": "Acute pancreatitis in Hong Kong.", "content": "A series of 311 Chinese patients with acute pancreatitis admitted to Queen Mary Hospital, Hong Kong, over a 10-year period is reviewed. Biliary tract disease was associated with pancreatitis in 52.4 per cent of patients and 77.9 per cent of them had stones, mud or parasites in the common bile duct. Fever and jaundice were present in 55 per cent and 41.2 per cent of patients respectively. Because of the prevalence of recurrent pyogenic cholangitis among the indigenous population, emergency operation, with the aim of common duct decompression, was conducted in 54.3 per cent of patients during the acute episode, with a mortality rate of 14.8 per cent. Five of 142 patients (3.5 per cent) died whilst on conservative treatment and all 5 had haemorrhagic pancreatitis. The overall mortality rate was 9.6 per cent. Exploration of the common bile duct, which was carried out in 57.4 per cent of patients in the acute phase, was not associated with a higher mortality than when laparotomy alone was performed, and 19 patients had sphincteroplasty without any death. Subtotal pancreatectomy was performed in 2 patients with haemorrhagic pancreatitis with 1 death.", "contents": "Acute pancreatitis in Hong Kong. A series of 311 Chinese patients with acute pancreatitis admitted to Queen Mary Hospital, Hong Kong, over a 10-year period is reviewed. Biliary tract disease was associated with pancreatitis in 52.4 per cent of patients and 77.9 per cent of them had stones, mud or parasites in the common bile duct. Fever and jaundice were present in 55 per cent and 41.2 per cent of patients respectively. Because of the prevalence of recurrent pyogenic cholangitis among the indigenous population, emergency operation, with the aim of common duct decompression, was conducted in 54.3 per cent of patients during the acute episode, with a mortality rate of 14.8 per cent. Five of 142 patients (3.5 per cent) died whilst on conservative treatment and all 5 had haemorrhagic pancreatitis. The overall mortality rate was 9.6 per cent. Exploration of the common bile duct, which was carried out in 57.4 per cent of patients in the acute phase, was not associated with a higher mortality than when laparotomy alone was performed, and 19 patients had sphincteroplasty without any death. Subtotal pancreatectomy was performed in 2 patients with haemorrhagic pancreatitis with 1 death."}
{"id": "PMID:466021", "title": "Haemorrhagic cholecystitis.", "content": "Two cases of haemobilia due to haemorrhagic cholecystitis are presented; only 28 other cases have been described. As there are so many more dramatic causes and treatment for haemobilia it is important for the surgeon to recognize that a common operation will suffice for this rare condition. In both cases bacteria were grown from the bile and it is to be hoped that bile culture will be recorded more commonly in future in cases of this interesting condition.", "contents": "Haemorrhagic cholecystitis. Two cases of haemobilia due to haemorrhagic cholecystitis are presented; only 28 other cases have been described. As there are so many more dramatic causes and treatment for haemobilia it is important for the surgeon to recognize that a common operation will suffice for this rare condition. In both cases bacteria were grown from the bile and it is to be hoped that bile culture will be recorded more commonly in future in cases of this interesting condition."}
{"id": "PMID:466022", "title": "Primary hydatid cyst of the gallbladder.", "content": "An unusual case of hydatid cyst of the gallbladder is presented. The difficulties in diagnosis are discussed. There is no other report of such a cyst in the world literature.", "contents": "Primary hydatid cyst of the gallbladder. An unusual case of hydatid cyst of the gallbladder is presented. The difficulties in diagnosis are discussed. There is no other report of such a cyst in the world literature."}
{"id": "PMID:466023", "title": "Splenic cysts: a review of 6 cases.", "content": "Benign cysts of the spleen are rare. Six cases have been managed in this hospital in the past 25 years. The history, physical findings and investigations are reviewed. It is suggested that splenectomy is the treatment of choice as it carries a low morbidity and mortality.", "contents": "Splenic cysts: a review of 6 cases. Benign cysts of the spleen are rare. Six cases have been managed in this hospital in the past 25 years. The history, physical findings and investigations are reviewed. It is suggested that splenectomy is the treatment of choice as it carries a low morbidity and mortality."}
{"id": "PMID:466024", "title": "H2-antagonists in the treatment of recurrent ulceration after vagotomy.", "content": "Twenty-two patients with recurrent ulceration following vagotomy with and without a drainage procedure have been treated with histamine H2-antagonists and followed for up to 3 years. Four of a group of 6 patients responded to a single therapeutic course. Ten of 16 patients assigned to long term maintenance treatment remain symptom-free. Cimetidine may be an alternative to further surgery in the treatment of some patients with recurrent ulceration after vagotomy.", "contents": "H2-antagonists in the treatment of recurrent ulceration after vagotomy. Twenty-two patients with recurrent ulceration following vagotomy with and without a drainage procedure have been treated with histamine H2-antagonists and followed for up to 3 years. Four of a group of 6 patients responded to a single therapeutic course. Ten of 16 patients assigned to long term maintenance treatment remain symptom-free. Cimetidine may be an alternative to further surgery in the treatment of some patients with recurrent ulceration after vagotomy."}
{"id": "PMID:466025", "title": "The excretion of alkaline ribonuclease by children undergoing surgery.", "content": "The excretion of alkaline ribonuclease has been measured in the urine of two groups of children after major operations. The excretion was significantly increased in all patients after open operations on the heart and in many of those who were operated on soon after birth for anomalies in the intestine. It is suggested that these elevations may indicate a disturbance of pancreatic function.", "contents": "The excretion of alkaline ribonuclease by children undergoing surgery. The excretion of alkaline ribonuclease has been measured in the urine of two groups of children after major operations. The excretion was significantly increased in all patients after open operations on the heart and in many of those who were operated on soon after birth for anomalies in the intestine. It is suggested that these elevations may indicate a disturbance of pancreatic function."}
{"id": "PMID:466028", "title": "The role of thrombolytic therapy in the management of phlegmasia caerulea dolens.", "content": "Two cases of phlegmasia caerulea dolens with peripheral venous gangrene treated with streptokinase are presented. Both patients had excellent results, beyond expectation. The literature is reviewed and the symptomatology, aetiology, pathogenesis and current thoughts on treatment are discussed.", "contents": "The role of thrombolytic therapy in the management of phlegmasia caerulea dolens. Two cases of phlegmasia caerulea dolens with peripheral venous gangrene treated with streptokinase are presented. Both patients had excellent results, beyond expectation. The literature is reviewed and the symptomatology, aetiology, pathogenesis and current thoughts on treatment are discussed."}
{"id": "PMID:466030", "title": "The morbidity of carotid endarterectomy.", "content": "The results of 72 carotid endarterectomy operations carried out by three consultant surgeons were analysed. Thirty-eight operations (32.8 per cent) were performed in patients with mild or resolving stroke and 33 operations (45.9 per cent) in patients with transient ischaemic attacks and/or amaurosis fugax. Five patients (7 per cent) died in the early postoperative period, 2 as a result of strokes and the others from cardiac causes. Seventy-six per cent of patients had a satisfactory long term result, remaining free from storke, blindness or recurrent transient ischaemic attacks over the follow-up period of 1--7 years. There were 10 patients (13.8 per cent), including 2 who died, who developed early postoperative neurological deficits; 4 (5.5 per cent) had transient defects and 6 (8.3 per cent) had permanent deficits. Of these patients, 4 had proved occlusions of the reconstructed artery. In 3 patients, cerebral embolization during operation was suspected as the cause. There were, in addition, 2 asymptomatic patients whose reconstruction was shown to be occluded on follow-up postoperative angiography. The continued absence of symptoms, therefore, is no guarantee that the reconstruction is patent and some more objective method of assessment is necessary if the results of carotid endarterectomy are to be critically analysed. The incidence of neurological deficits could, it is believed, be reduced by greater experience with the operation, the routine use of peroperative intra-arterial shunting and peroperative angiography to confirm the patency of the reconstruction.", "contents": "The morbidity of carotid endarterectomy. The results of 72 carotid endarterectomy operations carried out by three consultant surgeons were analysed. Thirty-eight operations (32.8 per cent) were performed in patients with mild or resolving stroke and 33 operations (45.9 per cent) in patients with transient ischaemic attacks and/or amaurosis fugax. Five patients (7 per cent) died in the early postoperative period, 2 as a result of strokes and the others from cardiac causes. Seventy-six per cent of patients had a satisfactory long term result, remaining free from storke, blindness or recurrent transient ischaemic attacks over the follow-up period of 1--7 years. There were 10 patients (13.8 per cent), including 2 who died, who developed early postoperative neurological deficits; 4 (5.5 per cent) had transient defects and 6 (8.3 per cent) had permanent deficits. Of these patients, 4 had proved occlusions of the reconstructed artery. In 3 patients, cerebral embolization during operation was suspected as the cause. There were, in addition, 2 asymptomatic patients whose reconstruction was shown to be occluded on follow-up postoperative angiography. The continued absence of symptoms, therefore, is no guarantee that the reconstruction is patent and some more objective method of assessment is necessary if the results of carotid endarterectomy are to be critically analysed. The incidence of neurological deficits could, it is believed, be reduced by greater experience with the operation, the routine use of peroperative intra-arterial shunting and peroperative angiography to confirm the patency of the reconstruction."}
{"id": "PMID:466032", "title": "The clinical relevance of radiologically revealed Paget's disease of bone (osteitis deformans).", "content": "Radiological and hospital records of 400 patients with Paget's disease that was shown radiologically have been examined in an effort to establish the clinical significance of the disease. Twenty-eight per cent of the patients were considered to have symptoms related to their disease, the most frequent symptom being bone pain, deformity and fracture. It appears that Paget's disease may predispose to the formation of urinary calculi. Malignant change and neurological and cardiovascular complications are rare, and osteitis deformans does not appear to predispose to osteoarthrosis.", "contents": "The clinical relevance of radiologically revealed Paget's disease of bone (osteitis deformans). Radiological and hospital records of 400 patients with Paget's disease that was shown radiologically have been examined in an effort to establish the clinical significance of the disease. Twenty-eight per cent of the patients were considered to have symptoms related to their disease, the most frequent symptom being bone pain, deformity and fracture. It appears that Paget's disease may predispose to the formation of urinary calculi. Malignant change and neurological and cardiovascular complications are rare, and osteitis deformans does not appear to predispose to osteoarthrosis."}
{"id": "PMID:466035", "title": "Distribution of plasma-bound hydroxyproline in breast cancer, benign breast disease and healthy females.", "content": "In normal adult females, patients with benign breast disease and patients with breast cancer, hydroxyproline (OHPro) was found in the plasma attached to the protein fractions which were separated by Sephadex gel filtration. In addition, a distinct moiety, probably a protein sub-unit, containing substantial amounts of this imino acid, occurred in between gel filtration fractions III and IV. Quantitative differences in the OHPro content of these various fractions were observed in the groups of patients studied. Significant elevations in the peptide-bound OHPro (fraction IV) were found only in patients with breast cancer and the level appeared to correlate with the degree of advancement of the disease. Similar findings relate to the OHPro content of the sub-protein moiety which is found between gel filtration fractions III and IV. A reduced level of high molecular weight hyproprotein (fraction I) was found in the breast cancer group.", "contents": "Distribution of plasma-bound hydroxyproline in breast cancer, benign breast disease and healthy females. In normal adult females, patients with benign breast disease and patients with breast cancer, hydroxyproline (OHPro) was found in the plasma attached to the protein fractions which were separated by Sephadex gel filtration. In addition, a distinct moiety, probably a protein sub-unit, containing substantial amounts of this imino acid, occurred in between gel filtration fractions III and IV. Quantitative differences in the OHPro content of these various fractions were observed in the groups of patients studied. Significant elevations in the peptide-bound OHPro (fraction IV) were found only in patients with breast cancer and the level appeared to correlate with the degree of advancement of the disease. Similar findings relate to the OHPro content of the sub-protein moiety which is found between gel filtration fractions III and IV. A reduced level of high molecular weight hyproprotein (fraction I) was found in the breast cancer group."}
{"id": "PMID:466036", "title": "Midline cancer of the floor of the mouth: role of suprahyoid dissection.", "content": "Of 37 patients with squamous cell carcinoma of the floor of the mouth seen during a 5-year period, 28 were treated primarily by surgery. Ten of the 28 patients presented with midline cancers measuring between 1.0 and 3 cm (T1 and T2). Three of these patients had local excision only. Metastases to the submandibular lymph nodes later occurred in 1 of these 3 patients. The remaining 7 patients with midline lesions had wide excision of the tumour, marginal resection of the mandible and bilateral suprahyoid node dissection. Of these 7 patients 4 remained free of disease for periods ranging between 2 and 5 years. Two of these had lymph node metastases. These results suggest that en bloc excision of relatively small midline cancers (i.e. not exceeding 2 cm (T1) in largest diameter) with bilateral suprahoid node dissection gives effective control of these lesions.", "contents": "Midline cancer of the floor of the mouth: role of suprahyoid dissection. Of 37 patients with squamous cell carcinoma of the floor of the mouth seen during a 5-year period, 28 were treated primarily by surgery. Ten of the 28 patients presented with midline cancers measuring between 1.0 and 3 cm (T1 and T2). Three of these patients had local excision only. Metastases to the submandibular lymph nodes later occurred in 1 of these 3 patients. The remaining 7 patients with midline lesions had wide excision of the tumour, marginal resection of the mandible and bilateral suprahyoid node dissection. Of these 7 patients 4 remained free of disease for periods ranging between 2 and 5 years. Two of these had lymph node metastases. These results suggest that en bloc excision of relatively small midline cancers (i.e. not exceeding 2 cm (T1) in largest diameter) with bilateral suprahoid node dissection gives effective control of these lesions."}
{"id": "PMID:466038", "title": "Pancreatogram changes in patients with calculous biliary disease.", "content": "A successful pancreatogram was obtained at endoscopic retrograde cholangiopancreatography (ERCP) in 53 patients with calculous biliary disease. Twenty-eight patients presented with jaundice and 25 with pain. In both groups there was a high incidence of pancreatogram abnormalities (47 and 48 per cent respectively). These findings demonstrate that the pancreas is often abnormal in the presence of complicated calculous biliary disease even though there may be no recent clinical evidence of pancreatitis and suggest that asymptomatic chronic pancreatitis may be common. The clinical significance of the abnormalities is discussed.", "contents": "Pancreatogram changes in patients with calculous biliary disease. A successful pancreatogram was obtained at endoscopic retrograde cholangiopancreatography (ERCP) in 53 patients with calculous biliary disease. Twenty-eight patients presented with jaundice and 25 with pain. In both groups there was a high incidence of pancreatogram abnormalities (47 and 48 per cent respectively). These findings demonstrate that the pancreas is often abnormal in the presence of complicated calculous biliary disease even though there may be no recent clinical evidence of pancreatitis and suggest that asymptomatic chronic pancreatitis may be common. The clinical significance of the abnormalities is discussed."}
{"id": "PMID:466039", "title": "Chronic relapsing pancreatitis: a review of 64 cases.", "content": "A collected series of 64 cases of chronic relapsing pancreatitis is reported. Analysis of this material reveals several points of interest. There is a difference in the aetiological spectrum in Britain when compared with reports from France and the United States. In particular, nearly half the British cases were idiopathic. The clinical presentation and the age and sex ratios also varied with aetiology. Endoscopic retrograde cannulation of the pancreatic duct was of little value in confirming a diagnosis of pancreatitis in the problem case. This investigation did, however, demonstrate that a widespread dilatation of the pancreatic duct was a minority finding. In those patients with alcoholic pancreatitis follow-up studies have shown that, if the addiction can be broken, there is a 75 per cent chance that pain will diminish or disappear with the passage of time. The main indication for surgical intervention was severe pain and this study has shown that if strict criteria are observed, a worthwhile relief of symptoms can be achieved. In particular, subtotal pancreatectomy produced good results in up to 85 per cent of cases, although with an appreciable short term postoperative morbidity.", "contents": "Chronic relapsing pancreatitis: a review of 64 cases. A collected series of 64 cases of chronic relapsing pancreatitis is reported. Analysis of this material reveals several points of interest. There is a difference in the aetiological spectrum in Britain when compared with reports from France and the United States. In particular, nearly half the British cases were idiopathic. The clinical presentation and the age and sex ratios also varied with aetiology. Endoscopic retrograde cannulation of the pancreatic duct was of little value in confirming a diagnosis of pancreatitis in the problem case. This investigation did, however, demonstrate that a widespread dilatation of the pancreatic duct was a minority finding. In those patients with alcoholic pancreatitis follow-up studies have shown that, if the addiction can be broken, there is a 75 per cent chance that pain will diminish or disappear with the passage of time. The main indication for surgical intervention was severe pain and this study has shown that if strict criteria are observed, a worthwhile relief of symptoms can be achieved. In particular, subtotal pancreatectomy produced good results in up to 85 per cent of cases, although with an appreciable short term postoperative morbidity."}
{"id": "PMID:466041", "title": "Does obstructive jaundice adversely affect wound healing?", "content": "The effect of obstructive jaundice on wound healing has been investigated in an experimental study of abdominal wounds in rats following ligation and division of the common bile duct. Animals were jaundiced for 2 weeks before a second operation at which the abdominal wounds were made. The wounds in jaundiced and control animals showed no significant differences in mechanical strength during a 21-day period of study but there was a significant delay in the accumulation of collagen in the wounds of jaundiced animals. The findings suggest that the biochemical changes in the wounds of jaundiced animals did not interfere with wound repair and cast doubt on the thesis that jaundice has an adverse effect on wound healing.", "contents": "Does obstructive jaundice adversely affect wound healing? The effect of obstructive jaundice on wound healing has been investigated in an experimental study of abdominal wounds in rats following ligation and division of the common bile duct. Animals were jaundiced for 2 weeks before a second operation at which the abdominal wounds were made. The wounds in jaundiced and control animals showed no significant differences in mechanical strength during a 21-day period of study but there was a significant delay in the accumulation of collagen in the wounds of jaundiced animals. The findings suggest that the biochemical changes in the wounds of jaundiced animals did not interfere with wound repair and cast doubt on the thesis that jaundice has an adverse effect on wound healing."}
{"id": "PMID:466042", "title": "The surgical treatment of axillary hyperhidrosis.", "content": "Axillary hyperhidrosis is a common condition and young people often suffer unnecessarily for many years in spite of the availability of good surgical methods for cure. We describe our own modification of Pettersson and Str\u00f6mb\u00e4ck's (1970) method of excision of the sweat glands. During the years 1972-6 218 axillary operations have been performed on 109 patients at the surgical clinic in V\u00e4ster\u00e5s for axillary hyperhidrosis. We reviewed 104 of these and found that 71 patients were completely satisfied and only 7 unsatisfied. Twelve patients wanted to be reoperated either because they still sweated or because they were unhappy with their scars. Thus, we found that overall the surgical methods are safe, simple and give good results.", "contents": "The surgical treatment of axillary hyperhidrosis. Axillary hyperhidrosis is a common condition and young people often suffer unnecessarily for many years in spite of the availability of good surgical methods for cure. We describe our own modification of Pettersson and Str\u00f6mb\u00e4ck's (1970) method of excision of the sweat glands. During the years 1972-6 218 axillary operations have been performed on 109 patients at the surgical clinic in V\u00e4ster\u00e5s for axillary hyperhidrosis. We reviewed 104 of these and found that 71 patients were completely satisfied and only 7 unsatisfied. Twelve patients wanted to be reoperated either because they still sweated or because they were unhappy with their scars. Thus, we found that overall the surgical methods are safe, simple and give good results."}
{"id": "PMID:466043", "title": "Congenital chylothorax: report of unique case associated with 'H' -type tracheo-oesophageal fistula.", "content": "A case of congenital chylothorax associated with a congenital 'H'-type tracheo-oesophageal fistula is reported. The development of the thoracic duct is described and the possible embryological association with tracheo-oesophageal fistula is discussed. The management of the case is reviewed.", "contents": "Congenital chylothorax: report of unique case associated with 'H' -type tracheo-oesophageal fistula. A case of congenital chylothorax associated with a congenital 'H'-type tracheo-oesophageal fistula is reported. The development of the thoracic duct is described and the possible embryological association with tracheo-oesophageal fistula is discussed. The management of the case is reviewed."}
{"id": "PMID:466045", "title": "Absorption from the jejunum is increased by forward and backward pacing.", "content": "In 4 dogs a 75-cm segment of jejunum was isolated from the intestinal stream. Stimulating electrodes were implanted at each end of the segment and recording electrodes at intervals between. The two ends of the segment were fashioned as stomas on the anterior abdominal wall. After recovery, the electrical activity of the segment was continuously recorded, whilst a solution of 100 mmol/l glucose and 90 mmol/l NaCl was infused into the proximal stoma at 2.9 ml/min. Effluent from the distal stoma was collected in 5-min aliquots for three consecutive 30-min periods. In the second period electrical stimuli were given via either the proximal or the distal bipolar electrodes to entrain pacesetter potentials. Both forward and backward electrical pacing decreased the output of water, glucose and sodium from the jejunal segment, but the decrease was significantly greater with backward pacing. We conclude that pacing a segment of jejunum backwards with electrical stimuli produces a greater increase in absorption from the segment than pacing it forwards, a finding which has therapeutic implications in patients with the short bowel syndrome.", "contents": "Absorption from the jejunum is increased by forward and backward pacing. In 4 dogs a 75-cm segment of jejunum was isolated from the intestinal stream. Stimulating electrodes were implanted at each end of the segment and recording electrodes at intervals between. The two ends of the segment were fashioned as stomas on the anterior abdominal wall. After recovery, the electrical activity of the segment was continuously recorded, whilst a solution of 100 mmol/l glucose and 90 mmol/l NaCl was infused into the proximal stoma at 2.9 ml/min. Effluent from the distal stoma was collected in 5-min aliquots for three consecutive 30-min periods. In the second period electrical stimuli were given via either the proximal or the distal bipolar electrodes to entrain pacesetter potentials. Both forward and backward electrical pacing decreased the output of water, glucose and sodium from the jejunal segment, but the decrease was significantly greater with backward pacing. We conclude that pacing a segment of jejunum backwards with electrical stimuli produces a greater increase in absorption from the segment than pacing it forwards, a finding which has therapeutic implications in patients with the short bowel syndrome."}
{"id": "PMID:466046", "title": "An experimental evaluation of segmental reversal after massive small bowel resection.", "content": "The technique of reversal of a distal segment of residual small intestine was applied to the management of massive resection. A controlled evaluation was carried out in dogs into the effects of segmental reversal on the proximal remnant after the distal 75 per cent of the small bowel had been resected. The variables studied were survival, body weight, glyceryl tri(palmitate-1-14C) absorption and mouth-to-caecum transit time. Animals subjected to resection alone had accelerated intestinal transit, poor absorption, serious weight loss and a high early mortality. It was clearly demonstrated that segmental reversal after resection produced marked and progressive delay in transit time from mouth to caecum, sustained improvement in fat absorption and body weight and enhanced survival.", "contents": "An experimental evaluation of segmental reversal after massive small bowel resection. The technique of reversal of a distal segment of residual small intestine was applied to the management of massive resection. A controlled evaluation was carried out in dogs into the effects of segmental reversal on the proximal remnant after the distal 75 per cent of the small bowel had been resected. The variables studied were survival, body weight, glyceryl tri(palmitate-1-14C) absorption and mouth-to-caecum transit time. Animals subjected to resection alone had accelerated intestinal transit, poor absorption, serious weight loss and a high early mortality. It was clearly demonstrated that segmental reversal after resection produced marked and progressive delay in transit time from mouth to caecum, sustained improvement in fat absorption and body weight and enhanced survival."}
{"id": "PMID:466047", "title": "Transcutaneous defunctioning colostomy.", "content": "The technique is described for constructing an occlusive tube colostomy which requires no second operative procedure for closure. The advantages of this method are discussed and it is recommended as a superior alternative to loop colostomy or caecostomy for defunctioning the unobstructed distal bowel.", "contents": "Transcutaneous defunctioning colostomy. The technique is described for constructing an occlusive tube colostomy which requires no second operative procedure for closure. The advantages of this method are discussed and it is recommended as a superior alternative to loop colostomy or caecostomy for defunctioning the unobstructed distal bowel."}
{"id": "PMID:466049", "title": "The effect of levamisole on postoperative immunosuppression.", "content": "Cell mediated immunity was studied before and after surgery in patients with colorectal cancer. Thymus-derived (T) lymphocytes were enumerated and their non-specific activity was measured by assessing phytohaemagglutinin (PHA)-induced blastogenesis. Specific T lymphocyte activity was measured by leucocyte migration inhibition in response to purified protein derivative of tuberculin (PPD) and autochthonous tumour extract. One group of patients received levamisole postoperatively and the other group consisted of controls. There was a more rapid return to preoperative levels of leucocyte migration inhibition to both antigens by the levamisole group. However, levamisole administration had only a marginal effect on the postoperative depression of cell counts and did not restore depressed lymphoblast transformation.", "contents": "The effect of levamisole on postoperative immunosuppression. Cell mediated immunity was studied before and after surgery in patients with colorectal cancer. Thymus-derived (T) lymphocytes were enumerated and their non-specific activity was measured by assessing phytohaemagglutinin (PHA)-induced blastogenesis. Specific T lymphocyte activity was measured by leucocyte migration inhibition in response to purified protein derivative of tuberculin (PPD) and autochthonous tumour extract. One group of patients received levamisole postoperatively and the other group consisted of controls. There was a more rapid return to preoperative levels of leucocyte migration inhibition to both antigens by the levamisole group. However, levamisole administration had only a marginal effect on the postoperative depression of cell counts and did not restore depressed lymphoblast transformation."}
{"id": "PMID:466050", "title": "Reduction in road fatalities and injuries after legislation for compulsory wearing of seat belts: experience in Victoria and the rest of Australia.", "content": "In 1970 the Government of the State of Victoria became the first in the 'western' world to introduce legislation for compulsory wearing of seat belts. Within 14 months the other Australian states followed. Seat belt wearing rates increased to attain 90 per cent in 1977. This paper presents an evaluation of changes during the period 1955--77 in the incidence of road crash fatalities and injuries for vehicle occupants in Victoria and the rest of Australia. The introduction of the legislation in Victoria and subsequently in the other Australian states coincided with significant and marked decreases in driver and passenger fatality and injury rates (numbers per 10 000 registered vehicles). The decreases were enough to reduce the total numbers of vehicle occupant fatalities and injuries for all post-legislation years in Victoria. The improvements have been maintained throughout the entire post-legislation period. The legislation did not apply to child passengers of less than 8 years of age and the frequency of seat belt or harness wearing among them remained low. These children did not share in the overall improvement for passengers. The Australian experience supports the view that legislation for compulsory wearing of seat belts is the single most effective method available for the protection of vehicle occupants in road crashes.", "contents": "Reduction in road fatalities and injuries after legislation for compulsory wearing of seat belts: experience in Victoria and the rest of Australia. In 1970 the Government of the State of Victoria became the first in the 'western' world to introduce legislation for compulsory wearing of seat belts. Within 14 months the other Australian states followed. Seat belt wearing rates increased to attain 90 per cent in 1977. This paper presents an evaluation of changes during the period 1955--77 in the incidence of road crash fatalities and injuries for vehicle occupants in Victoria and the rest of Australia. The introduction of the legislation in Victoria and subsequently in the other Australian states coincided with significant and marked decreases in driver and passenger fatality and injury rates (numbers per 10 000 registered vehicles). The decreases were enough to reduce the total numbers of vehicle occupant fatalities and injuries for all post-legislation years in Victoria. The improvements have been maintained throughout the entire post-legislation period. The legislation did not apply to child passengers of less than 8 years of age and the frequency of seat belt or harness wearing among them remained low. These children did not share in the overall improvement for passengers. The Australian experience supports the view that legislation for compulsory wearing of seat belts is the single most effective method available for the protection of vehicle occupants in road crashes."}
{"id": "PMID:466055", "title": "Vagotomy and pyloric dilatation in chronic duodenal ulceration.", "content": "A total of 101 patients suffering from duodenal ulcer underwent truncal vagotomy (TV) combined with pyloric dilatation (PD). They were followed up over six years, and the results were found to compare favourably with those in patients who underwent alternative surgical measures. Before any revisionary surgery 79 patients were classified as Visick grades I plus II. The incidence of recurrent ulceration was 4%. Side effects were noticeably less common than in patients in whom a drainage procedure had been performed, and overall results were compared with those reported for groups of patients treated by proximal gastric vagotomy. The combination of TV and PD is commended on account of its simplicity, safety, and effectiveness at a time when medical treatment for duodenal ulcer is becoming more specific and increasingly effective.", "contents": "Vagotomy and pyloric dilatation in chronic duodenal ulceration. A total of 101 patients suffering from duodenal ulcer underwent truncal vagotomy (TV) combined with pyloric dilatation (PD). They were followed up over six years, and the results were found to compare favourably with those in patients who underwent alternative surgical measures. Before any revisionary surgery 79 patients were classified as Visick grades I plus II. The incidence of recurrent ulceration was 4%. Side effects were noticeably less common than in patients in whom a drainage procedure had been performed, and overall results were compared with those reported for groups of patients treated by proximal gastric vagotomy. The combination of TV and PD is commended on account of its simplicity, safety, and effectiveness at a time when medical treatment for duodenal ulcer is becoming more specific and increasingly effective."}
{"id": "PMID:466056", "title": "Proximal gastric vagotomy, fundoplication, and lesser-curve necrosis.", "content": "Out of 400 patients who underwent proximal gastric vagotomy (PGV), three developed lesser-curve necrosis (LCN) leading to perforation within the first seven days. In each case diagnosis was delayed but the patient survived after a second operation. In each an associated Nissen fundoplication had been carried out; we used the combined operation in only 33 patients. Delayed LCN occurred in a patient who had undergone splenectomy at the time of the PGV and in a fifth patient treated elsewhere who had also undergone fundoplication. These findings indicate that early postoperative gastric distension with gas, not readily voided after fundoplication, may aggravate local vascular factors and predispose to LCN. We suggest that PGV combined with fundoplication may be dangerous.", "contents": "Proximal gastric vagotomy, fundoplication, and lesser-curve necrosis. Out of 400 patients who underwent proximal gastric vagotomy (PGV), three developed lesser-curve necrosis (LCN) leading to perforation within the first seven days. In each case diagnosis was delayed but the patient survived after a second operation. In each an associated Nissen fundoplication had been carried out; we used the combined operation in only 33 patients. Delayed LCN occurred in a patient who had undergone splenectomy at the time of the PGV and in a fifth patient treated elsewhere who had also undergone fundoplication. These findings indicate that early postoperative gastric distension with gas, not readily voided after fundoplication, may aggravate local vascular factors and predispose to LCN. We suggest that PGV combined with fundoplication may be dangerous."}
{"id": "PMID:466057", "title": "Renal function after long-term treatment with lithium.", "content": "Daily urine volumes, plasma creatinine concentrations, and creatinine clearance were measured in 106 patients with unipolar and bipolar affective disorders attending a \"lithium\" clinic. Urine volumes exceeded 3.51 in only six patients, plasma creatinine concentrations exceeded 150 mumol/1 (1.7 mg/100 ml) in only five, and creatinine clearance was below 50 ml/min in 16. Renal function was assessed by measuring creatinine clearance and renal tubular function, including response to 20 hours of water deprivation, in a representative sample of 30 patients from the lithium clinic and 30 psychiatric patients matched for age and sex who were taking other psychotropic drugs. Creatinine clearance and tubular function, including urine osmolality after water deprivation, were not significantly different between the two groups. Urinary excretion of arginine vasopressin (AVP), however, was much greater in the lithium-treated patients, who therefore had a diminished tubular responsiveness to AVP. The findings do not support suggestions that long-term lithium treatment results in seriously impaired renal function, renal damage, and polyuria. Compared with other series, however, the patients were being maintained with low serum lithium concentrations, which apparently area as effective prophylactically as higher concentrations.", "contents": "Renal function after long-term treatment with lithium. Daily urine volumes, plasma creatinine concentrations, and creatinine clearance were measured in 106 patients with unipolar and bipolar affective disorders attending a \"lithium\" clinic. Urine volumes exceeded 3.51 in only six patients, plasma creatinine concentrations exceeded 150 mumol/1 (1.7 mg/100 ml) in only five, and creatinine clearance was below 50 ml/min in 16. Renal function was assessed by measuring creatinine clearance and renal tubular function, including response to 20 hours of water deprivation, in a representative sample of 30 patients from the lithium clinic and 30 psychiatric patients matched for age and sex who were taking other psychotropic drugs. Creatinine clearance and tubular function, including urine osmolality after water deprivation, were not significantly different between the two groups. Urinary excretion of arginine vasopressin (AVP), however, was much greater in the lithium-treated patients, who therefore had a diminished tubular responsiveness to AVP. The findings do not support suggestions that long-term lithium treatment results in seriously impaired renal function, renal damage, and polyuria. Compared with other series, however, the patients were being maintained with low serum lithium concentrations, which apparently area as effective prophylactically as higher concentrations."}
{"id": "PMID:466067", "title": "Disposable syringes for insulin injection.", "content": "Results of a questionnaire on use of glass syringes among diabetic patients showed considerable variation in methods of keeping the syringes and in the duration of their useful life. Thirty patients took part in an investigation in which each patient used the same disposable syringe in place of the glass syringe for up to two months. No clinical or bacteriological evidence of infection was found. Used in this way, disposable syringes were less expensive than glass syringes. They are lighter than glass syringes, less susceptible to damage, and more easily carried on journeys.", "contents": "Disposable syringes for insulin injection. Results of a questionnaire on use of glass syringes among diabetic patients showed considerable variation in methods of keeping the syringes and in the duration of their useful life. Thirty patients took part in an investigation in which each patient used the same disposable syringe in place of the glass syringe for up to two months. No clinical or bacteriological evidence of infection was found. Used in this way, disposable syringes were less expensive than glass syringes. They are lighter than glass syringes, less susceptible to damage, and more easily carried on journeys."}
{"id": "PMID:466101", "title": "Survival of patients ventilated in an intensive therapy unit.", "content": "The survival of patients who were artificially ventilated in an intensive therapy unit (ITU) of a district general hospital was studied. The first 100 such patients admitted were followed up for at least four years. Of these, 67 survived treatment in the ITU, 47 were discharged from hospital, and 30 survived four years. (Survival in the ITU of patients who did not require ventilation was 89%.) The effects of age, duration of ventilation, and disease were studied, and a higher survival was found in children, in patients ventilated for under 24 hours, and in patients admitted with drug overdosage. Survival was poor in patients aged over 75 and in those who required ventilation after cardiac arrest or myocardial infarction or for chronic obstructive lung disease. The cost of a bed in an ITUs to be used to the greatest effect it is important to define those patients who are most likely to benefit from the facilities they offer.", "contents": "Survival of patients ventilated in an intensive therapy unit. The survival of patients who were artificially ventilated in an intensive therapy unit (ITU) of a district general hospital was studied. The first 100 such patients admitted were followed up for at least four years. Of these, 67 survived treatment in the ITU, 47 were discharged from hospital, and 30 survived four years. (Survival in the ITU of patients who did not require ventilation was 89%.) The effects of age, duration of ventilation, and disease were studied, and a higher survival was found in children, in patients ventilated for under 24 hours, and in patients admitted with drug overdosage. Survival was poor in patients aged over 75 and in those who required ventilation after cardiac arrest or myocardial infarction or for chronic obstructive lung disease. The cost of a bed in an ITUs to be used to the greatest effect it is important to define those patients who are most likely to benefit from the facilities they offer."}
{"id": "PMID:466102", "title": "Pneumoperitoneum associated with artificial ventilation.", "content": "Four adults injured in serious road-traffic accidents developed pneumoperitoneum after artificial ventilation. No case could be attributed to injury or to perforation of a hollow viscus in the belly, but doubt about this in one patient resulted in exploratory laparotomy. In three patients the origin of intraperitoneal air was considered to be leakage through minute ruptures in alveoli subjected to the stress of artificial ventilation, with air tracking to the mediastinum, pleural space, subcutaneous tissues of the neck and chest, and peritoneal cavity. The fourth patient later developed herniation of the stomach through a rupture in the diaphragm, the presence of a pneumothorax on the same side suggesting direct passage of air through the diaphragm. Pneumoperitoneum induced by artificial ventilation is probably more common than reports suggest and should be distinguished clinically and radiologically from that caused by rupture or perforation of a hollow viscus. This will reduce the number of needles laparotomies performed on patients who are already seriously ill.", "contents": "Pneumoperitoneum associated with artificial ventilation. Four adults injured in serious road-traffic accidents developed pneumoperitoneum after artificial ventilation. No case could be attributed to injury or to perforation of a hollow viscus in the belly, but doubt about this in one patient resulted in exploratory laparotomy. In three patients the origin of intraperitoneal air was considered to be leakage through minute ruptures in alveoli subjected to the stress of artificial ventilation, with air tracking to the mediastinum, pleural space, subcutaneous tissues of the neck and chest, and peritoneal cavity. The fourth patient later developed herniation of the stomach through a rupture in the diaphragm, the presence of a pneumothorax on the same side suggesting direct passage of air through the diaphragm. Pneumoperitoneum induced by artificial ventilation is probably more common than reports suggest and should be distinguished clinically and radiologically from that caused by rupture or perforation of a hollow viscus. This will reduce the number of needles laparotomies performed on patients who are already seriously ill."}
{"id": "PMID:466103", "title": "Unknown primary adenocarcinoma: incidence of overinvestigation and natural history.", "content": "Out of 1300 patients referred to a medical oncology unit, there were 87 with metastatic cancer in whom a primary tumour site was not evident from the history and after physical examination and chest radiography had been carried out. An analysis of the investigations performed in these patients and their results showed that in only eight of the 87 patients did non-surgical investigations at presentation determine the primary site. In two patients it was identified by diagnostic laparotomy, and in a further 13 clinical follow-up led to recognition of the primary tumour site before death. Few investigations should be performed in patients in whom the primary site is known since they have a low yield, and in our population identifying the primary tumour did not improve the outcome or alter management. Treatable tumours should be excluded, and this may be done in most cases by simple blood tests, particularly those measuring acid phosphatase activity and other tumour markers.", "contents": "Unknown primary adenocarcinoma: incidence of overinvestigation and natural history. Out of 1300 patients referred to a medical oncology unit, there were 87 with metastatic cancer in whom a primary tumour site was not evident from the history and after physical examination and chest radiography had been carried out. An analysis of the investigations performed in these patients and their results showed that in only eight of the 87 patients did non-surgical investigations at presentation determine the primary site. In two patients it was identified by diagnostic laparotomy, and in a further 13 clinical follow-up led to recognition of the primary tumour site before death. Few investigations should be performed in patients in whom the primary site is known since they have a low yield, and in our population identifying the primary tumour did not improve the outcome or alter management. Treatable tumours should be excluded, and this may be done in most cases by simple blood tests, particularly those measuring acid phosphatase activity and other tumour markers."}
{"id": "PMID:466104", "title": "Raised blood pressure and plasma noradrenaline concentrations in teenagers and young adults selected from an open population.", "content": "Plasma noradrenaline (PNA) concentrations were measured in 38 subjects aged 13-23, who were followed up for two to four years after an initial blood-pressure (BP) reading of 140/90 mm Hg or over was obtained, and in 39 age-matched controls from the same open population. Subjects who were hypertensive when the PNA concentration was measured had a significantly higher concentration (351 +/- SE 26 pg/ml) compared with their controls (248 +/- 29 pg/ml). Furthermore, in those subjects in whom the mean arterial pressure decreased by under 5% during the follow-up period the mean concentration was 363 +/- 27 pg/ml, compared with 271 +/- 29 pg/ml in their controls. PNA concentrations and systolic BP were positively correlated. A positive association between PNA concentrations and age was observed in the controls but not the subjects with hypertension, owing to the higher concentrations in younger hypertensive subjects.", "contents": "Raised blood pressure and plasma noradrenaline concentrations in teenagers and young adults selected from an open population. Plasma noradrenaline (PNA) concentrations were measured in 38 subjects aged 13-23, who were followed up for two to four years after an initial blood-pressure (BP) reading of 140/90 mm Hg or over was obtained, and in 39 age-matched controls from the same open population. Subjects who were hypertensive when the PNA concentration was measured had a significantly higher concentration (351 +/- SE 26 pg/ml) compared with their controls (248 +/- 29 pg/ml). Furthermore, in those subjects in whom the mean arterial pressure decreased by under 5% during the follow-up period the mean concentration was 363 +/- 27 pg/ml, compared with 271 +/- 29 pg/ml in their controls. PNA concentrations and systolic BP were positively correlated. A positive association between PNA concentrations and age was observed in the controls but not the subjects with hypertension, owing to the higher concentrations in younger hypertensive subjects."}
{"id": "PMID:466107", "title": "Spinal-cord compression in myeloma.", "content": "Clinical records of 47 patients in whom spinal-cord compression was the presenting feature of plasma-cell myeloma were analysed retrospectively. Patients were referred during 1954-78. Median survival was 30 months and prognosis was best for those in whom the site of cord compression was the thoracic region. Early laminectomy and decompression followed by adequate radiotherapy resulted in complete or good partial response in over a third of patients who presented with complete paraplegia. Improvements in supportive care and more effective chemotherapy allow spinal-cord compression in myeloma to be treated promptly and vigorously, thus improving duration and quality of survival in a substantial proportion of patients.", "contents": "Spinal-cord compression in myeloma. Clinical records of 47 patients in whom spinal-cord compression was the presenting feature of plasma-cell myeloma were analysed retrospectively. Patients were referred during 1954-78. Median survival was 30 months and prognosis was best for those in whom the site of cord compression was the thoracic region. Early laminectomy and decompression followed by adequate radiotherapy resulted in complete or good partial response in over a third of patients who presented with complete paraplegia. Improvements in supportive care and more effective chemotherapy allow spinal-cord compression in myeloma to be treated promptly and vigorously, thus improving duration and quality of survival in a substantial proportion of patients."}
{"id": "PMID:466138", "title": "Randomised comparison of methyldopa and oxprenolol for treatment of hypertension in pregnancy.", "content": "Fifty-three pregnant women with moderately severe hypertension were randomly allocated to treatment with methyldopa or oxprenolol. There were no significant differences between the groups in age, height, weight, parity, or stage of gestation at the start of treatment. The outcome of pregnancy was better in the group treated with oxprenolol, with greater maternal plasma volume expansion and placental and fetal growth. No intrauterine deaths occurred in either group, and antepartum fetal distress, detected by oxytocin challenge testing, was evident in only one patient, who received methyldopa. This infant, and one other in the methyldopa group, died in the neonatal period. No neonatal deaths occurred in the oxprenolol-treated group. Even in this small number of patients these results were considerably better than those in untreated women with hypertension of similar severity. Apgar scores in both groups were equivalent at birth, while blood sugar concentrations were higher in the oxprenolol group. Oxprenolol appears to be safe and effective in controlling hypertension during pregnancy. There was no evidence of harmful effects on the fetus, and oxprenolol may offer a selective advantage over methyldopa for fetal growth and wellbeing in utero.", "contents": "Randomised comparison of methyldopa and oxprenolol for treatment of hypertension in pregnancy. Fifty-three pregnant women with moderately severe hypertension were randomly allocated to treatment with methyldopa or oxprenolol. There were no significant differences between the groups in age, height, weight, parity, or stage of gestation at the start of treatment. The outcome of pregnancy was better in the group treated with oxprenolol, with greater maternal plasma volume expansion and placental and fetal growth. No intrauterine deaths occurred in either group, and antepartum fetal distress, detected by oxytocin challenge testing, was evident in only one patient, who received methyldopa. This infant, and one other in the methyldopa group, died in the neonatal period. No neonatal deaths occurred in the oxprenolol-treated group. Even in this small number of patients these results were considerably better than those in untreated women with hypertension of similar severity. Apgar scores in both groups were equivalent at birth, while blood sugar concentrations were higher in the oxprenolol group. Oxprenolol appears to be safe and effective in controlling hypertension during pregnancy. There was no evidence of harmful effects on the fetus, and oxprenolol may offer a selective advantage over methyldopa for fetal growth and wellbeing in utero."}
{"id": "PMID:466139", "title": "Reasons for requesting radiographs in an accident department.", "content": "A prospective study of 500 patients was performed to determine the reasons for requesting radiographs in an accident and emergency department. Most examinations were requested either to confirm a clinically suspected abnormality or because of difficulty in excluding a significant bone injury on clinical grounds alone. Several requests were also made to reassure the patient. Medicolegal reasons were relatively few, and those made purely because the doctor feared litigation probably accounted for only 5% of requests. Undue emphasis on the medicolegal aspects of accident and emergency radiography in the United Kingdom is unhelpful in that it directs attention away from the real reasons for x-ray referral. Although a reduction in the number of x-ray examinations is desirable on the grounds of expense and radiation exposure it is likely to be obtained only by improving experience and acumen in the clinical assessment of injuries.", "contents": "Reasons for requesting radiographs in an accident department. A prospective study of 500 patients was performed to determine the reasons for requesting radiographs in an accident and emergency department. Most examinations were requested either to confirm a clinically suspected abnormality or because of difficulty in excluding a significant bone injury on clinical grounds alone. Several requests were also made to reassure the patient. Medicolegal reasons were relatively few, and those made purely because the doctor feared litigation probably accounted for only 5% of requests. Undue emphasis on the medicolegal aspects of accident and emergency radiography in the United Kingdom is unhelpful in that it directs attention away from the real reasons for x-ray referral. Although a reduction in the number of x-ray examinations is desirable on the grounds of expense and radiation exposure it is likely to be obtained only by improving experience and acumen in the clinical assessment of injuries."}
{"id": "PMID:466140", "title": "General anaesthesia in sickle-cell disease.", "content": "General anaesthesia was administered on 284 occasions to 200 patients with sickle-cell disease at one hospital during July 1958 to June 1978. No intraoperative but six postoperative deaths occurred. The management of anaesthesia may have contributed to two of the postoperative deaths. Clinically uneventful anaesthesia did not appear to provoke severe sickling crises or to be responsible for mortality, but a contribution to postoperative morbidity could not be excluded. A simple, careful anaesthetic technique and selective but not routine blood transfusion appears to be associated with minimal anaesthetic morbidity and mortality in patients with sickle-cell disease.", "contents": "General anaesthesia in sickle-cell disease. General anaesthesia was administered on 284 occasions to 200 patients with sickle-cell disease at one hospital during July 1958 to June 1978. No intraoperative but six postoperative deaths occurred. The management of anaesthesia may have contributed to two of the postoperative deaths. Clinically uneventful anaesthesia did not appear to provoke severe sickling crises or to be responsible for mortality, but a contribution to postoperative morbidity could not be excluded. A simple, careful anaesthetic technique and selective but not routine blood transfusion appears to be associated with minimal anaesthetic morbidity and mortality in patients with sickle-cell disease."}
{"id": "PMID:466141", "title": "Whooping cough after stopping pertussis immunisation.", "content": "An epidemic of whooping cough occurred in a rural practice in Shetland, containing 144 children under 16. Before July 1974 all children were immunised against pertussis, but after that date immunisation was stopped. Of the 134 children studied, 93 had been immunised. Sixty-five of the children developed whooping cough. The incidence of infection was similar in those who had and had not been immunised. The incidence was also similar in those born before and after July 1974. There was no evidence to support the routine use of pertussis immunisation in rural Shetland.", "contents": "Whooping cough after stopping pertussis immunisation. An epidemic of whooping cough occurred in a rural practice in Shetland, containing 144 children under 16. Before July 1974 all children were immunised against pertussis, but after that date immunisation was stopped. Of the 134 children studied, 93 had been immunised. Sixty-five of the children developed whooping cough. The incidence of infection was similar in those who had and had not been immunised. The incidence was also similar in those born before and after July 1974. There was no evidence to support the routine use of pertussis immunisation in rural Shetland."}
{"id": "PMID:466144", "title": "Minor operations in general practice.", "content": "An analysis of minor operations performed in one general practice showed that one doctor doing an average of only four minor operations a week can save the area health authority over 15 000 pounds a year.", "contents": "Minor operations in general practice. An analysis of minor operations performed in one general practice showed that one doctor doing an average of only four minor operations a week can save the area health authority over 15 000 pounds a year."}
{"id": "PMID:466174", "title": "Raised serum human chorionic gonadotrophin concentrations in hyperemesis gravidarum.", "content": "Serum human chorionic gonadotrophin (HCG) concentrations were determined by radioimmunoassay with an antiserum specific to HCG beta-subunit in 42 patients with hyperemesis gravidarum and 115 women with normal pregnancies. Mean concentrations (+/- SE of mean) were higher in the women with hyperemesis gravidarum at 7-8 weeks (40.8 +/- 5.2 IU/ml v 22.1 +/- 1.4 IU/ml; P less than 0.001), 9-11 weeks (38.1 +/- 2.3 IU/ml v 27.1 +/- 2.1 IU/ml; P less than 0.0025), and 12-14 weeks of gestation (35.9 +/- 4.2 IU/ml v 25.1 +/- 1.7 IU/ml; P less than 0.005), but there was no difference between the two groups at 15-20 weeks of gestation. In the hyperemesis gravidarum group primigravid women had a higher (P less than 0.005) mean HCG concentration (41.8 +/- 4.0 IU/ml) than multigravid women (32.2 +/- 2.3 IU/ml). The results suggest a causal relation between a high serum HCG concentration and hyperemesis gravidarum.", "contents": "Raised serum human chorionic gonadotrophin concentrations in hyperemesis gravidarum. Serum human chorionic gonadotrophin (HCG) concentrations were determined by radioimmunoassay with an antiserum specific to HCG beta-subunit in 42 patients with hyperemesis gravidarum and 115 women with normal pregnancies. Mean concentrations (+/- SE of mean) were higher in the women with hyperemesis gravidarum at 7-8 weeks (40.8 +/- 5.2 IU/ml v 22.1 +/- 1.4 IU/ml; P less than 0.001), 9-11 weeks (38.1 +/- 2.3 IU/ml v 27.1 +/- 2.1 IU/ml; P less than 0.0025), and 12-14 weeks of gestation (35.9 +/- 4.2 IU/ml v 25.1 +/- 1.7 IU/ml; P less than 0.005), but there was no difference between the two groups at 15-20 weeks of gestation. In the hyperemesis gravidarum group primigravid women had a higher (P less than 0.005) mean HCG concentration (41.8 +/- 4.0 IU/ml) than multigravid women (32.2 +/- 2.3 IU/ml). The results suggest a causal relation between a high serum HCG concentration and hyperemesis gravidarum."}
{"id": "PMID:466180", "title": "Peer review of consultations in primary care: the use of audiovisual recordings.", "content": "A rating scale was designed to measure performance in interviewing techniques in primary care. Peer review of audiovisual recordings of their consultations showed that a group of experienced general practitioners achieved significantly higher scores on the rating scale compared with a group of similarly experienced general practitioners who did not observe their recordings. The higher scores were obtained at the expense of longer consultations. The traditional five-minute appointment system in general practice needs to be reconsidered if an improved interviewing technique results in a more favourable outcome.", "contents": "Peer review of consultations in primary care: the use of audiovisual recordings. A rating scale was designed to measure performance in interviewing techniques in primary care. Peer review of audiovisual recordings of their consultations showed that a group of experienced general practitioners achieved significantly higher scores on the rating scale compared with a group of similarly experienced general practitioners who did not observe their recordings. The higher scores were obtained at the expense of longer consultations. The traditional five-minute appointment system in general practice needs to be reconsidered if an improved interviewing technique results in a more favourable outcome."}
{"id": "PMID:466209", "title": "Spread of steroid-containing foam after intrarectal administration.", "content": "The spread of a steroid-containing foam from the rectum was studied in eight normal controls and eight patients with ulcerative colitis proved by biopsy. A standard dose of foam was labelled with technetium-99m adsorbed on to microspheres of Amberlite resin. Immediately after the foam was administered the extent of spread varied considerably, though in no instance did it extend beyond the rectosigmoid. Further scans at 120 and 240 minutes showed no further spread. It is concluded that a steroid-containing foam has a topical effect only on the rectal mucosa.", "contents": "Spread of steroid-containing foam after intrarectal administration. The spread of a steroid-containing foam from the rectum was studied in eight normal controls and eight patients with ulcerative colitis proved by biopsy. A standard dose of foam was labelled with technetium-99m adsorbed on to microspheres of Amberlite resin. Immediately after the foam was administered the extent of spread varied considerably, though in no instance did it extend beyond the rectosigmoid. Further scans at 120 and 240 minutes showed no further spread. It is concluded that a steroid-containing foam has a topical effect only on the rectal mucosa."}
{"id": "PMID:466210", "title": "Improved glucose control in maturity-onset diabetes treated with high-carbohydrate-modified fat diet.", "content": "Fourteen patients with established maturity-onset diabetes were treated as outpatients with a high-carbohydrate-(about 60% of total daily energy requirements)-modified fat diet (ratio of polyunsaturated fatty acids to other fatty acids greater than or equal to 1:1) for six weeks. Commercially available and acceptable cereal foods and tuberous vegetables high in both digestible and non-digestible carbohydrates were used. Simple sugars were restricted. Compared with their usual, low-carbohydrate diabetic diet this diet resulted in a fall in basal plasma glucose concentration (average of values measured at 0300, 0500, and 0700), mean preprandial plasma glucose concentration (average of values measured at 0800, 1230, and 1730), and percentage of glycosylated haemoglobin. Modifying dietary fat also decreased the fasting plasma cholesterol concentration. The findings suggest that it is no longer justifiable to prescribe a low-carbohydrate diet for maturity-onset diabetes.", "contents": "Improved glucose control in maturity-onset diabetes treated with high-carbohydrate-modified fat diet. Fourteen patients with established maturity-onset diabetes were treated as outpatients with a high-carbohydrate-(about 60% of total daily energy requirements)-modified fat diet (ratio of polyunsaturated fatty acids to other fatty acids greater than or equal to 1:1) for six weeks. Commercially available and acceptable cereal foods and tuberous vegetables high in both digestible and non-digestible carbohydrates were used. Simple sugars were restricted. Compared with their usual, low-carbohydrate diabetic diet this diet resulted in a fall in basal plasma glucose concentration (average of values measured at 0300, 0500, and 0700), mean preprandial plasma glucose concentration (average of values measured at 0800, 1230, and 1730), and percentage of glycosylated haemoglobin. Modifying dietary fat also decreased the fasting plasma cholesterol concentration. The findings suggest that it is no longer justifiable to prescribe a low-carbohydrate diet for maturity-onset diabetes."}
{"id": "PMID:466211", "title": "An epidemiological study of oral contraceptives and breast cancer.", "content": "During 1968-77, 707 women aged 16-50 years with newly diagnosed breast cancer and 707 matched controls were interviewed at eight teaching hospitals in London and Oxford about their use of oral contraceptives. Eighty-six of the patients with breast cancer were matched with controls with gall-bladder disease; these subjects were omitted from the main analyses, which thus related to 621 case-control pairs.The results were reassuring. A few statistically significant differences in oral contraceptive use were found between the breast cancer and control groups, but the data were subdivided in many ways, so that some \"significant\" differences would have been expected to occur by chance. The only subgroup in which the evidence for a positive association between pill use and breast cancer was at all convincing comprised women aged 46-50 years, but trends in those aged 41-45 were by and large in the opposite direction and results of combined analysis gave no cause for concern.Information on clinical stage was available for 487 patients with breast cancer treated before the end of 1975. Those who had never used oral contraceptives had appreciably more advanced tumours at presentation than those who had been using the pill during the year before detection of the lump, while past users of the pill occupied an intermediate position. This difference in staging was reflected in the pattern of survival. Oral contraceptives may have had a beneficial effect on tumour growth and spread, though diagnostic bias could not be definitely excluded.", "contents": "An epidemiological study of oral contraceptives and breast cancer. During 1968-77, 707 women aged 16-50 years with newly diagnosed breast cancer and 707 matched controls were interviewed at eight teaching hospitals in London and Oxford about their use of oral contraceptives. Eighty-six of the patients with breast cancer were matched with controls with gall-bladder disease; these subjects were omitted from the main analyses, which thus related to 621 case-control pairs.The results were reassuring. A few statistically significant differences in oral contraceptive use were found between the breast cancer and control groups, but the data were subdivided in many ways, so that some \"significant\" differences would have been expected to occur by chance. The only subgroup in which the evidence for a positive association between pill use and breast cancer was at all convincing comprised women aged 46-50 years, but trends in those aged 41-45 were by and large in the opposite direction and results of combined analysis gave no cause for concern.Information on clinical stage was available for 487 patients with breast cancer treated before the end of 1975. Those who had never used oral contraceptives had appreciably more advanced tumours at presentation than those who had been using the pill during the year before detection of the lump, while past users of the pill occupied an intermediate position. This difference in staging was reflected in the pattern of survival. Oral contraceptives may have had a beneficial effect on tumour growth and spread, though diagnostic bias could not be definitely excluded."}
{"id": "PMID:466212", "title": "Effects of manufacturing oral contraceptives on blood clotting.", "content": "In monitoring the effects of industrial exposure resulting from the pharmaceutical manufacture of oestrogen-progestogen combinations by coagulation studies acceleration of some clotting tests was found. The most pronounced changes were in workers most closely associated with the industrial process. Less pronounced changes were found in women employees not closely concerned with the processing and may have been secondary to the postmenopausal bleeding to which they were prone. A safer work procedure elaborated by the Employment Medical Advisory Service was monitored by clotting studies for over a year but the three most highly exposed subjects showed no substantial improvement.", "contents": "Effects of manufacturing oral contraceptives on blood clotting. In monitoring the effects of industrial exposure resulting from the pharmaceutical manufacture of oestrogen-progestogen combinations by coagulation studies acceleration of some clotting tests was found. The most pronounced changes were in workers most closely associated with the industrial process. Less pronounced changes were found in women employees not closely concerned with the processing and may have been secondary to the postmenopausal bleeding to which they were prone. A safer work procedure elaborated by the Employment Medical Advisory Service was monitored by clotting studies for over a year but the three most highly exposed subjects showed no substantial improvement."}
{"id": "PMID:466247", "title": "Asthma due to industrial use of chloramine.", "content": "Seven brewery workers developed asthmatic symptoms after using chloramine (chloramine-T) powder as a sterilising agent. They gave positive weal and flare reactions to skin-prick tests with solutions of chloramine at strengths that caused no reactions in unexposed controls. The symptoms did not recur once the men had been removed from areas in which chloramine was handled. As well as causing irritant effects, inhaling dry or liquid aerosols of chloramine may cause sensitisation, with workers being prone to allergic asthma on reexposure. In view of this, measures should be taken to ensure that chloramine is not inhaled.", "contents": "Asthma due to industrial use of chloramine. Seven brewery workers developed asthmatic symptoms after using chloramine (chloramine-T) powder as a sterilising agent. They gave positive weal and flare reactions to skin-prick tests with solutions of chloramine at strengths that caused no reactions in unexposed controls. The symptoms did not recur once the men had been removed from areas in which chloramine was handled. As well as causing irritant effects, inhaling dry or liquid aerosols of chloramine may cause sensitisation, with workers being prone to allergic asthma on reexposure. In view of this, measures should be taken to ensure that chloramine is not inhaled."}
{"id": "PMID:466248", "title": "Long-term parenteral exposure to mercury in patients with hypogammaglobulinaemia.", "content": "Patients with hypogammaglobulinaemia commonly receive regular long-term replacement therapy with a concentrate of pooled normal human immunoglobulin G (IgG) containing an organic mercury compound (thiomersal) as a preservative. In 26 such patients the total estimated mercury dosage received ranged from 4 to 734 mg (mean 157 mg) over treatment periods of six months to 17 years (mean 6.5 years). Nineteen patients (73%) had raised urine mercury concentrations, but no correlation was found between urine mercury and the age of the patient, the IgG dose, or the duration of treatment. Urine mercury concentrations are often used to control exposure and evaluate risks in exposed subjects. Hence most patients with hypogammaglobulinaemia are theoretically at risk from mercury exposure, although no clinical evidence of toxicity is yet apparent.", "contents": "Long-term parenteral exposure to mercury in patients with hypogammaglobulinaemia. Patients with hypogammaglobulinaemia commonly receive regular long-term replacement therapy with a concentrate of pooled normal human immunoglobulin G (IgG) containing an organic mercury compound (thiomersal) as a preservative. In 26 such patients the total estimated mercury dosage received ranged from 4 to 734 mg (mean 157 mg) over treatment periods of six months to 17 years (mean 6.5 years). Nineteen patients (73%) had raised urine mercury concentrations, but no correlation was found between urine mercury and the age of the patient, the IgG dose, or the duration of treatment. Urine mercury concentrations are often used to control exposure and evaluate risks in exposed subjects. Hence most patients with hypogammaglobulinaemia are theoretically at risk from mercury exposure, although no clinical evidence of toxicity is yet apparent."}
{"id": "PMID:466249", "title": "Relation between medicines sweetened with sucrose and dental disease.", "content": "The teeth of 44 children aged under 6 years who had been taking syrup medicines regularly for at least six months were compared with those of a control group of 47 children of similar ages. Dental disease was assessed by measuring dental caries, dental plaque, and gingivitis. The children who were receiving sucrose-based medicines had significantly more carious teeth and gingivitis. It is concluded that sucrose-based medicines continuously administered to children cause dental caries and gingivitis. Liquid medicines for children should be either unsweetened or sweetened with non-cariogenic substances.", "contents": "Relation between medicines sweetened with sucrose and dental disease. The teeth of 44 children aged under 6 years who had been taking syrup medicines regularly for at least six months were compared with those of a control group of 47 children of similar ages. Dental disease was assessed by measuring dental caries, dental plaque, and gingivitis. The children who were receiving sucrose-based medicines had significantly more carious teeth and gingivitis. It is concluded that sucrose-based medicines continuously administered to children cause dental caries and gingivitis. Liquid medicines for children should be either unsweetened or sweetened with non-cariogenic substances."}
{"id": "PMID:466256", "title": "Costs of unnecessary tests.", "content": "The relative importance of the history, examination, and routine and special investigations has been assessed in the diagnosis and management of 630 medical outpatients. The history was the most important factor in both diagnosis and management in cardiovascular, neurological, respiratory, urinary, and other miscellaneous problems, and decided 56% of all diagnoses and 46% of all management. Examination findings were of more limited value, accounting for only 17% of diagnostic and 17% of management decisions overall. Routine haematology and urine examinations were of minimal value, contributing to less than 1% of all diagnoses. Special investigations decided most of the diagnoses and management in alimentary and endocrine problems, but were less helpful in problems affecting all other systems. If a local survey in the Trent Region reflected the pattern of medical outpatient practice nationally, then pounds 1.25 million could be saved annually if routine investigation was discontinued on all patients whose problem had already been diagnosed on the basis of the history and the clinical examination.", "contents": "Costs of unnecessary tests. The relative importance of the history, examination, and routine and special investigations has been assessed in the diagnosis and management of 630 medical outpatients. The history was the most important factor in both diagnosis and management in cardiovascular, neurological, respiratory, urinary, and other miscellaneous problems, and decided 56% of all diagnoses and 46% of all management. Examination findings were of more limited value, accounting for only 17% of diagnostic and 17% of management decisions overall. Routine haematology and urine examinations were of minimal value, contributing to less than 1% of all diagnoses. Special investigations decided most of the diagnoses and management in alimentary and endocrine problems, but were less helpful in problems affecting all other systems. If a local survey in the Trent Region reflected the pattern of medical outpatient practice nationally, then pounds 1.25 million could be saved annually if routine investigation was discontinued on all patients whose problem had already been diagnosed on the basis of the history and the clinical examination."}
{"id": "PMID:466270", "title": "Anaerobic axillary abscess.", "content": "Fifty-two patients with axillary abscesses were seen during two years. Staphylococcus aureus was isolated from 34, anaerobic bacteria from 12, and skin flora from five; in one case the pus was sterile. Seven patients with hidradenitis suppurativa had recurrent infection with abscess formation, which was bilateral in three. Anaerobes were isolated in five of these cases and skin flora alone in two. Anaerobes are secondary invaders in this condition, and histological examination shows that the primary abnormality is obstruction of pilosebaceous follicles and apocrine glands, associated with keratin plugging of the follicles. Chemotherapy offers little hope of cure, although metronidazole removes the offensive smell of the discharge. Radical surgery is usually indicated.", "contents": "Anaerobic axillary abscess. Fifty-two patients with axillary abscesses were seen during two years. Staphylococcus aureus was isolated from 34, anaerobic bacteria from 12, and skin flora from five; in one case the pus was sterile. Seven patients with hidradenitis suppurativa had recurrent infection with abscess formation, which was bilateral in three. Anaerobes were isolated in five of these cases and skin flora alone in two. Anaerobes are secondary invaders in this condition, and histological examination shows that the primary abnormality is obstruction of pilosebaceous follicles and apocrine glands, associated with keratin plugging of the follicles. Chemotherapy offers little hope of cure, although metronidazole removes the offensive smell of the discharge. Radical surgery is usually indicated."}
{"id": "PMID:466286", "title": "Hospital outbreak of trimethoprim resistance in pathogenic coliform bacteria.", "content": "In an investigation of outbreak of infection caused by coliform bacilli with plasmid-mediated trimethoprim (TMP) resistance many patients were found to be asymptomatic carriers of TMP-resistant coliform bacilli. Analysis of factors predisposing to rectal carriage of these organisms showed that the most important was previous treatment with co-trimoxazole, a sulphonamide, or ampicillin. The outbreak was controlled by a policy restricting the antibiotics given. Geriatric units are an important source of hospital infection. When an outbreak occurs the logical sequence of steps to be taken is to monitor cases, identify the outbreaks, analyse the causative factors, plan corrective action jointly with laboratory staff, and monitor the outcome.", "contents": "Hospital outbreak of trimethoprim resistance in pathogenic coliform bacteria. In an investigation of outbreak of infection caused by coliform bacilli with plasmid-mediated trimethoprim (TMP) resistance many patients were found to be asymptomatic carriers of TMP-resistant coliform bacilli. Analysis of factors predisposing to rectal carriage of these organisms showed that the most important was previous treatment with co-trimoxazole, a sulphonamide, or ampicillin. The outbreak was controlled by a policy restricting the antibiotics given. Geriatric units are an important source of hospital infection. When an outbreak occurs the logical sequence of steps to be taken is to monitor cases, identify the outbreaks, analyse the causative factors, plan corrective action jointly with laboratory staff, and monitor the outcome."}
{"id": "PMID:466292", "title": "Induction of labour using prostaglandin E2 pessaries.", "content": "The routine method of induction at Queen Charlotte's Maternity Hospital is now by the use of prostaglandin E2 pessaries. The first 502 consecutive patients thus induced are presented: the caesarean section rate for a failed induction with an unfavourable cervix has fallen to 2%. The prostaglandin E2 pessary is highly efficient and acceptable for all cases in which a simple amniotomy will not suffice.", "contents": "Induction of labour using prostaglandin E2 pessaries. The routine method of induction at Queen Charlotte's Maternity Hospital is now by the use of prostaglandin E2 pessaries. The first 502 consecutive patients thus induced are presented: the caesarean section rate for a failed induction with an unfavourable cervix has fallen to 2%. The prostaglandin E2 pessary is highly efficient and acceptable for all cases in which a simple amniotomy will not suffice."}
{"id": "PMID:466329", "title": "Comparative trial of volunteer and professional treatments of dysphasia after stroke.", "content": "A study of the treatment of dysphasia after stroke compared the progress of two groups of disabled patients. One received conventional treatment from qualified speech therapists and the other from non-professional volunteers. Methods of assessing communication difficulties were also compared and the impact of aphasic illness on families examined. No important differences in the results of treatment were seen between the two groups. The volunteers, however, often had to assume some of the responsibilities of social workers, and transport to hospital created practical and economic problems. It is concluded that the two forms of treatment provide essentially the same benefit, although doubt must still remain because relatively few patients were studied.", "contents": "Comparative trial of volunteer and professional treatments of dysphasia after stroke. A study of the treatment of dysphasia after stroke compared the progress of two groups of disabled patients. One received conventional treatment from qualified speech therapists and the other from non-professional volunteers. Methods of assessing communication difficulties were also compared and the impact of aphasic illness on families examined. No important differences in the results of treatment were seen between the two groups. The volunteers, however, often had to assume some of the responsibilities of social workers, and transport to hospital created practical and economic problems. It is concluded that the two forms of treatment provide essentially the same benefit, although doubt must still remain because relatively few patients were studied."}
{"id": "PMID:466334", "title": "Methodology of monitored release of a new preparation: buprenorphine.", "content": "The analgesic agent Temgesic (buprenorphine) was made available under monitored release regulations for one year, which yielded data from 1736 doctors on 9123 patients. An analysis of 17 120 administrations of the drug confirmed the results of the pre-licensing clinical studies encompassing data from nine British hospitals on 483 patients. No important new adverse effects attributable to the product were observed, and apart from giving reassurance, the usefulness of monitored release is questioned in the light of the relatively small amount of additional information arising from it.", "contents": "Methodology of monitored release of a new preparation: buprenorphine. The analgesic agent Temgesic (buprenorphine) was made available under monitored release regulations for one year, which yielded data from 1736 doctors on 9123 patients. An analysis of 17 120 administrations of the drug confirmed the results of the pre-licensing clinical studies encompassing data from nine British hospitals on 483 patients. No important new adverse effects attributable to the product were observed, and apart from giving reassurance, the usefulness of monitored release is questioned in the light of the relatively small amount of additional information arising from it."}
{"id": "PMID:466335", "title": "Postoperative analgesia: a comparison of intravenous on-demand fentanyl with epidural bupivacaine.", "content": "In a randomised trial postoperative pain relief was provided by either epidural injections of bupivacaine or an infusion of fentanyl adjusted by the patient to achieve adequate pain relief. Both techniques produced satisfactory analgesia without respiratory depression after peripheral arterial surgery. The technique of infusing intravenously a potent analgesic in a dose adjusted by the patient appears to offer several advantages in postoperative care.", "contents": "Postoperative analgesia: a comparison of intravenous on-demand fentanyl with epidural bupivacaine. In a randomised trial postoperative pain relief was provided by either epidural injections of bupivacaine or an infusion of fentanyl adjusted by the patient to achieve adequate pain relief. Both techniques produced satisfactory analgesia without respiratory depression after peripheral arterial surgery. The technique of infusing intravenously a potent analgesic in a dose adjusted by the patient appears to offer several advantages in postoperative care."}
{"id": "PMID:466336", "title": "Gutter treatment for ingrowing toenails.", "content": "The gutter treatment for ingrowing toenails consists of introducing a small guard along the side of the toenail and requires only three outpatient attendances. The gutter is left in place for eight to 12 weeks and then removed by the patient. Two studies were carried out to establish the long-term results of the method. In one, a preliminary retrospective study, 13 out of 25 patients who had received the gutter treatment were cured after one year compared with five out of 15 patients in whom the toenail had been avulsed. In a randomised prospective study 20 out of 36 patients (56%) treated by the gutter method were cured after one year compared with 27 out of 32 (84%) in whom the wedge resection procedure had been used. The gutter treatment demands little skill and may be carried out in general practice. It gives an excellent cosmetic result with immediate pain relief and does not compromise further surgery should this be required, thus fulfilling the main requirements for the primary treatment of choice.", "contents": "Gutter treatment for ingrowing toenails. The gutter treatment for ingrowing toenails consists of introducing a small guard along the side of the toenail and requires only three outpatient attendances. The gutter is left in place for eight to 12 weeks and then removed by the patient. Two studies were carried out to establish the long-term results of the method. In one, a preliminary retrospective study, 13 out of 25 patients who had received the gutter treatment were cured after one year compared with five out of 15 patients in whom the toenail had been avulsed. In a randomised prospective study 20 out of 36 patients (56%) treated by the gutter method were cured after one year compared with 27 out of 32 (84%) in whom the wedge resection procedure had been used. The gutter treatment demands little skill and may be carried out in general practice. It gives an excellent cosmetic result with immediate pain relief and does not compromise further surgery should this be required, thus fulfilling the main requirements for the primary treatment of choice."}
{"id": "PMID:466337", "title": "Maternal smoking habits and congenital malformations: a population study.", "content": "Maternal smoking habits in 67 609 singleton pregnancies were examined. The overall incidence of congenital malformations was 2.8% in both non-smokers and smokers. On analysing congenital defects according to individual systems there was no significant difference in the incidence of malformations according to the number of cigarettes smoked, except for neural tube defects. Further analysis, taking social class into account, showed that neural tube defects were less common in non-smokers in social classes I and II. This study suggests that maternal smoking does not have teratogenic effects in the offspring, except in the case of neural tube defects, where the effect is at most modest.", "contents": "Maternal smoking habits and congenital malformations: a population study. Maternal smoking habits in 67 609 singleton pregnancies were examined. The overall incidence of congenital malformations was 2.8% in both non-smokers and smokers. On analysing congenital defects according to individual systems there was no significant difference in the incidence of malformations according to the number of cigarettes smoked, except for neural tube defects. Further analysis, taking social class into account, showed that neural tube defects were less common in non-smokers in social classes I and II. This study suggests that maternal smoking does not have teratogenic effects in the offspring, except in the case of neural tube defects, where the effect is at most modest."}
{"id": "PMID:466338", "title": "Autonomic neuropathy in rheumatoid arthritis.", "content": "Patients with seropositive and seronegative rheumatoid arthritis (RA) and age-matched controls were investigated for the presence of autonomic neuropathy. Significantly more patients with RA had abnormal autonomic function, suggesting that autonomic neuropathy occurs more commonly in RA than hitherto suspected. The existence of an autonomic neuropathy may be an important complicating factor in rheumatoid disease and may lead to increased morbidity and mortality.", "contents": "Autonomic neuropathy in rheumatoid arthritis. Patients with seropositive and seronegative rheumatoid arthritis (RA) and age-matched controls were investigated for the presence of autonomic neuropathy. Significantly more patients with RA had abnormal autonomic function, suggesting that autonomic neuropathy occurs more commonly in RA than hitherto suspected. The existence of an autonomic neuropathy may be an important complicating factor in rheumatoid disease and may lead to increased morbidity and mortality."}
{"id": "PMID:466344", "title": "Needle aspiration of the breast with immediate reporting of material.", "content": "Needle aspiration with immediate cytological reporting has been practised in a breast clinic for one year. Patients benefit by receiving immediately the provisional diagnosis and, when indicated, appointments for metastatic surveys. Close co-operation between surgeon and cytologist has resulted in increased skill in aspiration, better preparation of samples, and greater accuracy in interpretation of reports. Since 5% of clinically benign lesions have proved malignant, even on immediate reporting, we would recommend cytological examination of all breast lumps.", "contents": "Needle aspiration of the breast with immediate reporting of material. Needle aspiration with immediate cytological reporting has been practised in a breast clinic for one year. Patients benefit by receiving immediately the provisional diagnosis and, when indicated, appointments for metastatic surveys. Close co-operation between surgeon and cytologist has resulted in increased skill in aspiration, better preparation of samples, and greater accuracy in interpretation of reports. Since 5% of clinically benign lesions have proved malignant, even on immediate reporting, we would recommend cytological examination of all breast lumps."}
{"id": "PMID:466381", "title": "Ultrastructural study of satellite lymph nodes in syphilitic rabbits.", "content": "In an electron microscopy study of lymph nodes from syphilitic rabbits plasmablasts and plasma cells were unequivocally identified in germinal centres. Up to 20% of the plasma cells possessed unusual cytological features. Paracortical hypoplasia was shown to be associated with actively phagocytic histiocytes. These findings may reflect morphological correlates of the aberrant immune regulation which has been observed in infection with Treponema pallidum.", "contents": "Ultrastructural study of satellite lymph nodes in syphilitic rabbits. In an electron microscopy study of lymph nodes from syphilitic rabbits plasmablasts and plasma cells were unequivocally identified in germinal centres. Up to 20% of the plasma cells possessed unusual cytological features. Paracortical hypoplasia was shown to be associated with actively phagocytic histiocytes. These findings may reflect morphological correlates of the aberrant immune regulation which has been observed in infection with Treponema pallidum."}
{"id": "PMID:466382", "title": "Treatment monitoring in syphilis using the autoanalyser.", "content": "Treatment monitoring in syphilis using the automated Reiter protein complement fixation test and the automated reagin test was investigated over a two-year period. Clearly defined response patterns were demonstrated in primary and secondary syphilis and, to a lesser extent, in latent syphilis, thus facilitating the assessment of treatment and the identification of treatment failures and reinfection. No obvious treatment failures were detected in those patients receiving penicillin, but two failures were noted in a group of patients with secondary syphilis treated with doxycyline. The combination of two automated tests overcame some of the disadvantages inherent in the use of a single reagin test.", "contents": "Treatment monitoring in syphilis using the autoanalyser. Treatment monitoring in syphilis using the automated Reiter protein complement fixation test and the automated reagin test was investigated over a two-year period. Clearly defined response patterns were demonstrated in primary and secondary syphilis and, to a lesser extent, in latent syphilis, thus facilitating the assessment of treatment and the identification of treatment failures and reinfection. No obvious treatment failures were detected in those patients receiving penicillin, but two failures were noted in a group of patients with secondary syphilis treated with doxycyline. The combination of two automated tests overcame some of the disadvantages inherent in the use of a single reagin test."}
{"id": "PMID:466383", "title": "Rapid presumptive diagnosis of gonococcal urethritis in men by the limulus lysate test.", "content": "In an evaluation of the limulus assay as a method for detecting endotoxin in urethral exudates, positive results of urethral samples at a 1/200 dilution were obtained from 73 out of 73 patients with culture-positive gonococcal urethritis while negative results were obtained from 26 out of 27 patients with cuture-negative urethral specimens. A specimen from one patient, which gave negative results on Gram stain and culture, gave positive results to the limulus test. The overall accuracy of the limulus test for predicting culture results was 99% (p less than 0.001). Thus, in preliminary studies of otherwise healthy men, the results of the limulus assay correlated with those of biological methods for diagnosing urethral gonorrhoea; the test may, therefore, be of use in identifying cases of nongonococcal urethritis.", "contents": "Rapid presumptive diagnosis of gonococcal urethritis in men by the limulus lysate test. In an evaluation of the limulus assay as a method for detecting endotoxin in urethral exudates, positive results of urethral samples at a 1/200 dilution were obtained from 73 out of 73 patients with culture-positive gonococcal urethritis while negative results were obtained from 26 out of 27 patients with cuture-negative urethral specimens. A specimen from one patient, which gave negative results on Gram stain and culture, gave positive results to the limulus test. The overall accuracy of the limulus test for predicting culture results was 99% (p less than 0.001). Thus, in preliminary studies of otherwise healthy men, the results of the limulus assay correlated with those of biological methods for diagnosing urethral gonorrhoea; the test may, therefore, be of use in identifying cases of nongonococcal urethritis."}
{"id": "PMID:466384", "title": "Clinical pharmacological studies of amoxycillin: effect of probenecid.", "content": "In a study of eight, healthy adult volunteers given 3 g amoxycillin with or without 1g probenecid, significantly higher peak plasma levels of amoxycilin were recorded in the presence (34.96 microgram/ml) of probenecid than in its absence (22.72 microgram/ml). When plasma levels were plotted against time the mean area under the curve was significantly greater for subjects given probenecid than for those given amoxycillin alone. These findings suggest that 3g amoxycillin plus 1g probenecid provide better bioavailability than 3g amoxycillin alone. The plasma levels obtained were several times higher than the minimun inhibitory concentrations (MICs) of most strains of gonococci. Plasma levels in excess of the MICs for most strains were maintained for eight hours with both regimens, but the higher levels in the presence of probenecid support the better clinical results previously reported with this regimen.", "contents": "Clinical pharmacological studies of amoxycillin: effect of probenecid. In a study of eight, healthy adult volunteers given 3 g amoxycillin with or without 1g probenecid, significantly higher peak plasma levels of amoxycilin were recorded in the presence (34.96 microgram/ml) of probenecid than in its absence (22.72 microgram/ml). When plasma levels were plotted against time the mean area under the curve was significantly greater for subjects given probenecid than for those given amoxycillin alone. These findings suggest that 3g amoxycillin plus 1g probenecid provide better bioavailability than 3g amoxycillin alone. The plasma levels obtained were several times higher than the minimun inhibitory concentrations (MICs) of most strains of gonococci. Plasma levels in excess of the MICs for most strains were maintained for eight hours with both regimens, but the higher levels in the presence of probenecid support the better clinical results previously reported with this regimen."}
{"id": "PMID:466385", "title": "Reinfection with disseminated gonococcal disease.", "content": "A 20-year-old prostitute was admitted to hospital with joint pains and a macular and pustular rash on the fingers and feet on two occasions. Disseminated gonococcal infection was diagnosed and successfully treated.", "contents": "Reinfection with disseminated gonococcal disease. A 20-year-old prostitute was admitted to hospital with joint pains and a macular and pustular rash on the fingers and feet on two occasions. Disseminated gonococcal infection was diagnosed and successfully treated."}
{"id": "PMID:466396", "title": "A retrothalamic system of collateral fibers from the cerebral peduncle.", "content": "Ram\u00f3n y Cajal described a sizeable bundle of collateral fibers from the cerebral peduncle and called it 'faisceau de Forel'. This bundle has been consistently ignored by recent authors, possibly because of the difficulty to visualize it in transversal section. It is described here as the fasciculus prerubralis, or FPR. It originates at the level of the transition from the nucleus subthalamicus to the substantia nigra. In sagittal-stained sections of the mouse brain stained with the rapid Golgi method it can be seen following an ascending and nearly vertical course. It arches caudalwards, and appears to terminate in the rostral tegmentum. In the cat, Nauta-stained material proved that the corticofugal fibers which contribute to this system originate preponderantly, if not exclusively, in the pericruciate gyrus. Following lesions in this cortical area one could observe, in addition to the degenerating fibers of this bundle, another system of degenerating direct cortico-tegmental fibers which coursed toward the rostral tegmentum. It is not clear whether this second degenerating system resulted from the encroachment of passing fibers in the subjacent internal capsule. The tegmental prerubral collateral fibers from the cerebral peduncle were observed in mouse, rat and cat. Possibly, they could represent a general feature of the mammalian nervous system.", "contents": "A retrothalamic system of collateral fibers from the cerebral peduncle. Ram\u00f3n y Cajal described a sizeable bundle of collateral fibers from the cerebral peduncle and called it 'faisceau de Forel'. This bundle has been consistently ignored by recent authors, possibly because of the difficulty to visualize it in transversal section. It is described here as the fasciculus prerubralis, or FPR. It originates at the level of the transition from the nucleus subthalamicus to the substantia nigra. In sagittal-stained sections of the mouse brain stained with the rapid Golgi method it can be seen following an ascending and nearly vertical course. It arches caudalwards, and appears to terminate in the rostral tegmentum. In the cat, Nauta-stained material proved that the corticofugal fibers which contribute to this system originate preponderantly, if not exclusively, in the pericruciate gyrus. Following lesions in this cortical area one could observe, in addition to the degenerating fibers of this bundle, another system of degenerating direct cortico-tegmental fibers which coursed toward the rostral tegmentum. It is not clear whether this second degenerating system resulted from the encroachment of passing fibers in the subjacent internal capsule. The tegmental prerubral collateral fibers from the cerebral peduncle were observed in mouse, rat and cat. Possibly, they could represent a general feature of the mammalian nervous system."}
{"id": "PMID:466397", "title": "Neurochemical and histochemical studies of the effect of a lesion of the nucleus cuneiformis on the cholinergic innervation of discrete areas of the rat brain.", "content": "The innervation sites of the dorsal tegmental acetylcholinesterase (AChE)-containing pathway were examined in rats by combining histochemical and biochemical techniques. A lesion was placed in the nucleus cuneiformis (midbrain reticular formation) and brains were examined after 4 days survival for changes in AChE staining and choline acetyltransferase (ChAT) activity in discrete brain areas. An ipsilateral projection appears to exist to the anterior thalamic nuclei, lateral portion of the medial thalamic nucleus, parafascicular nucleus, pretectal nucleus, posterior thalamic nucleus, and deep layers of the superior colliculus. A possible bilateral innervation to the reticular nucleus of the thalamus and the dorsal and ventral lateral geniculates was found. The parallel use of AChE histochemistry and measurements of ChAT activity in discrete nuclei will be useful for future evaluation of cholinergic pathways.", "contents": "Neurochemical and histochemical studies of the effect of a lesion of the nucleus cuneiformis on the cholinergic innervation of discrete areas of the rat brain. The innervation sites of the dorsal tegmental acetylcholinesterase (AChE)-containing pathway were examined in rats by combining histochemical and biochemical techniques. A lesion was placed in the nucleus cuneiformis (midbrain reticular formation) and brains were examined after 4 days survival for changes in AChE staining and choline acetyltransferase (ChAT) activity in discrete brain areas. An ipsilateral projection appears to exist to the anterior thalamic nuclei, lateral portion of the medial thalamic nucleus, parafascicular nucleus, pretectal nucleus, posterior thalamic nucleus, and deep layers of the superior colliculus. A possible bilateral innervation to the reticular nucleus of the thalamus and the dorsal and ventral lateral geniculates was found. The parallel use of AChE histochemistry and measurements of ChAT activity in discrete nuclei will be useful for future evaluation of cholinergic pathways."}
{"id": "PMID:466398", "title": "The effect of penicillin on the release of gamma-aminobutyric acid from cerebral cortex slices.", "content": "Penicillin is known to block the depressant action of GABA on cortical and spinal neurons. In this study we examined the effect of penicillin on the release of endogenous amino acids from rat cerebral cortex slices. The principal finding was that penicillin blocked the potassium-stimulated release of GABA, a calcium-dependent process. It did not block the veratridine-stimulated release of GABA, a calcium-independent process. It did not affect the release of other putative transmitter amino acids. Inhibition of GABA release from nerve endings may explain in part the ability of penicillin to depress synaptic inhibitory mechanisms.", "contents": "The effect of penicillin on the release of gamma-aminobutyric acid from cerebral cortex slices. Penicillin is known to block the depressant action of GABA on cortical and spinal neurons. In this study we examined the effect of penicillin on the release of endogenous amino acids from rat cerebral cortex slices. The principal finding was that penicillin blocked the potassium-stimulated release of GABA, a calcium-dependent process. It did not block the veratridine-stimulated release of GABA, a calcium-independent process. It did not affect the release of other putative transmitter amino acids. Inhibition of GABA release from nerve endings may explain in part the ability of penicillin to depress synaptic inhibitory mechanisms."}
{"id": "PMID:466406", "title": "Localization of lumbar epaxial motoneurons in the rat.", "content": "In the rat, lateral longissimus and the lumbar transverso-spinalis muscles are lumbar trunk muscles, and participate in the lordosis reflex (female sexual posturing). Medial longissimus, the remaining major lumbar epaxial muscle, is a muscle of the proximal tail-tailbase. To allow an analysis of the motor control of lordosis, motoneurons for these muscles must be localized. Horseradish peroxidase (HRP) was injected into lumbar epaxial muscles of female rats. Following injection into medial longissimus, labeled cells were found posterior to the lumbar enlargement, ventrolaterally in the ventral horn. Following injections into lateral longissimus or into lumbar transverso-spinalis muscles, most labeled cells were found on the medial side of the ventral horn, extending through and anterior to the lumbar enlargement. Injections into lumbar transverso-spinalis muscles at more posterior levels led to more posterior locations of labeled cells. The distributions of the labeled cells agree with previous observations on innervation of the muscles. Additionally, the spinal cord was scanned for sites at which microstimulation produced visible twitches of medial longissimus or transverso-spinalis muscles. Locations of low threshold twitch sites were consistent with conclusions based on the HRP findings.", "contents": "Localization of lumbar epaxial motoneurons in the rat. In the rat, lateral longissimus and the lumbar transverso-spinalis muscles are lumbar trunk muscles, and participate in the lordosis reflex (female sexual posturing). Medial longissimus, the remaining major lumbar epaxial muscle, is a muscle of the proximal tail-tailbase. To allow an analysis of the motor control of lordosis, motoneurons for these muscles must be localized. Horseradish peroxidase (HRP) was injected into lumbar epaxial muscles of female rats. Following injection into medial longissimus, labeled cells were found posterior to the lumbar enlargement, ventrolaterally in the ventral horn. Following injections into lateral longissimus or into lumbar transverso-spinalis muscles, most labeled cells were found on the medial side of the ventral horn, extending through and anterior to the lumbar enlargement. Injections into lumbar transverso-spinalis muscles at more posterior levels led to more posterior locations of labeled cells. The distributions of the labeled cells agree with previous observations on innervation of the muscles. Additionally, the spinal cord was scanned for sites at which microstimulation produced visible twitches of medial longissimus or transverso-spinalis muscles. Locations of low threshold twitch sites were consistent with conclusions based on the HRP findings."}
{"id": "PMID:466408", "title": "A critical period in the development of tectal neurons in the chick, as revealed by early enucleation.", "content": "To further study the existence of a critical trophic period in the development of the chick optic tectum, during which the presence of retinal synapses is essential to the continued growth of tectal neurons, we have unilaterally enucleated embryos between stages 14--20 and allowed survival until stages 35--43. If the critical trophic period is between stages 40--44, as previously reported, then we reasoned that early removal of the eye might not have any effect on tectal development until the critical period. We assessed tectal neuron survival by staining for degeneration in the efferent projections of tectal neurons. In early enucleates, degeneration was present from stages 37--43, and the severity of the degeneration was much reduced in comparison to animals enucleated during the critical period. These findings substantiate the proposition that there is a critical period late in chick tectal development. However, because the degeneration in tectal projections is less intense than in animals enucleated during the critical period, we suggest that the early enucleation has permitted axons from the remaining eye to be routed to the deafferented tectum, where they may help to sustain a portion of the tectal neurons through the critical period. Moreover, the somewhat earlier appearance of degeneration in tectal efferent pathways of early enucleates suggests that a subtle trophic relationship between retina and tectum may exist prior to stage 40, even though this relationship is not revealed when enucleations are performed later, as between stages 35--40 (ref. 17).", "contents": "A critical period in the development of tectal neurons in the chick, as revealed by early enucleation. To further study the existence of a critical trophic period in the development of the chick optic tectum, during which the presence of retinal synapses is essential to the continued growth of tectal neurons, we have unilaterally enucleated embryos between stages 14--20 and allowed survival until stages 35--43. If the critical trophic period is between stages 40--44, as previously reported, then we reasoned that early removal of the eye might not have any effect on tectal development until the critical period. We assessed tectal neuron survival by staining for degeneration in the efferent projections of tectal neurons. In early enucleates, degeneration was present from stages 37--43, and the severity of the degeneration was much reduced in comparison to animals enucleated during the critical period. These findings substantiate the proposition that there is a critical period late in chick tectal development. However, because the degeneration in tectal projections is less intense than in animals enucleated during the critical period, we suggest that the early enucleation has permitted axons from the remaining eye to be routed to the deafferented tectum, where they may help to sustain a portion of the tectal neurons through the critical period. Moreover, the somewhat earlier appearance of degeneration in tectal efferent pathways of early enucleates suggests that a subtle trophic relationship between retina and tectum may exist prior to stage 40, even though this relationship is not revealed when enucleations are performed later, as between stages 35--40 (ref. 17)."}
{"id": "PMID:466409", "title": "Spike activity and histofluorescence correlated in the giant dopamine neurone of Planorbis corneus.", "content": "The relationship between catecholamine fluorescence and electrical activity of the nerve cell has been investigated in the giant dopamine neuron (GDN) of the left pedal ganglion of the European water snail, Planorbis corneus. Electrical recordings were performed in vitro with intracellular microelectrodes on 35 GDN. The ganglion was frozen to -195 degrees C with the electrode in situ and processed for histochemical microfluorimetry. The intensity of catecholamine fluorescence was measured over different places (42/cell) throughout the cytoplasm of the GDN. In order to investigate the temporal relation between histochemical and electrophysiological parameters, the activity of 21 GDN was changed by administration of nicotine to the bathing solution (10(-5)--10(-7) M). This treatment was followed by depolarization in most of the GDN, with increased firing in two thirds and decreased activity in one third of the spontaneously active cells, whereas hyperpolarization was seen in 4 GDN, accompanied by a decrease in firing. Acetylcholine (10(-5) M) tested on one GDN caused depolarization and increased firing. A signigicant positive correlation was found between the fluorescence intensity of individual GDN and the firing rate of these cells as observed during the last 60 sec or the last 10 sec before freezing. The correlation coefficient dropped markedly when the first rates of the 2nd, 3rd, or 4th and 5th min before freezing were correlated with cellular fluorescence intensity. Intensity was not correlated with the resting membrane potential recorded at the time of freezing. The intensity response to activation was not uniform throughout the cytoplasm of GDN. Neurons with increased mean fluorescence intensity regularly showed small clusters and cristae of intensely fluorescent material surrounded by less fluorescent parts of the cytoplasm. This morphological observation of increased intensity differences between cytoplasmic structures was confirmed by the statistics of the intensity values determined in individual cells. The present observations demonstrate the existence of a relation between cell firing and neuronal dopamine fluorescence at the level of the individual nerve cell. The activity immediately preceding the time of freezing of the cell appears to be most important. Certain components of the cytoplasm appear to react preferentially; their subcellular nature remains to be elucidated. The link between electrical activity and cellular dopamine fluorescene was found to be basically the same in the giatn invertebrate neuron and in the dopamine nerve cells of rat substantia nigra.", "contents": "Spike activity and histofluorescence correlated in the giant dopamine neurone of Planorbis corneus. The relationship between catecholamine fluorescence and electrical activity of the nerve cell has been investigated in the giant dopamine neuron (GDN) of the left pedal ganglion of the European water snail, Planorbis corneus. Electrical recordings were performed in vitro with intracellular microelectrodes on 35 GDN. The ganglion was frozen to -195 degrees C with the electrode in situ and processed for histochemical microfluorimetry. The intensity of catecholamine fluorescence was measured over different places (42/cell) throughout the cytoplasm of the GDN. In order to investigate the temporal relation between histochemical and electrophysiological parameters, the activity of 21 GDN was changed by administration of nicotine to the bathing solution (10(-5)--10(-7) M). This treatment was followed by depolarization in most of the GDN, with increased firing in two thirds and decreased activity in one third of the spontaneously active cells, whereas hyperpolarization was seen in 4 GDN, accompanied by a decrease in firing. Acetylcholine (10(-5) M) tested on one GDN caused depolarization and increased firing. A signigicant positive correlation was found between the fluorescence intensity of individual GDN and the firing rate of these cells as observed during the last 60 sec or the last 10 sec before freezing. The correlation coefficient dropped markedly when the first rates of the 2nd, 3rd, or 4th and 5th min before freezing were correlated with cellular fluorescence intensity. Intensity was not correlated with the resting membrane potential recorded at the time of freezing. The intensity response to activation was not uniform throughout the cytoplasm of GDN. Neurons with increased mean fluorescence intensity regularly showed small clusters and cristae of intensely fluorescent material surrounded by less fluorescent parts of the cytoplasm. This morphological observation of increased intensity differences between cytoplasmic structures was confirmed by the statistics of the intensity values determined in individual cells. The present observations demonstrate the existence of a relation between cell firing and neuronal dopamine fluorescence at the level of the individual nerve cell. The activity immediately preceding the time of freezing of the cell appears to be most important. Certain components of the cytoplasm appear to react preferentially; their subcellular nature remains to be elucidated. The link between electrical activity and cellular dopamine fluorescene was found to be basically the same in the giatn invertebrate neuron and in the dopamine nerve cells of rat substantia nigra."}
{"id": "PMID:466410", "title": "Three-dimensional organization of the endoplasmic reticulum in supraoptic neurons of the rat. A structural functional correlation.", "content": "Double impregnation staining of tissue was used to study on thick sections the three-dimensional configuration of the peripheral endoplasmic reticulum in neurosecretory neurons of the supraoptic nucleus in control and water-deprived rats. According to the spatial organization of the endoplasmic reticulum, two types of neurons are described in this report: Type I neurons which predominated in control rats (70%) exhibited characteristically large lamellar structures connected to loosely anastomosed tubules. In type II neurons, which prevailed in water-deprived rats (85%) the endoplasmic reticulum had the appearance of a highly-developed network of interconnected tubules, with no lamellar structures. Double impregnation staining combined with high resolution radioautography after [3H]leucine administration showed that the tubular configuration of the endoplasmic reticulum was the main active site of protein synthesis by contrast with the lamellar components, whose activity seemed poor. In terms of protein synthesis, the three-dimensional configuration of the peripheral endoplasmic reticulum of the supraoptic neurons appeared therefore to be closely connected with their functional state.", "contents": "Three-dimensional organization of the endoplasmic reticulum in supraoptic neurons of the rat. A structural functional correlation. Double impregnation staining of tissue was used to study on thick sections the three-dimensional configuration of the peripheral endoplasmic reticulum in neurosecretory neurons of the supraoptic nucleus in control and water-deprived rats. According to the spatial organization of the endoplasmic reticulum, two types of neurons are described in this report: Type I neurons which predominated in control rats (70%) exhibited characteristically large lamellar structures connected to loosely anastomosed tubules. In type II neurons, which prevailed in water-deprived rats (85%) the endoplasmic reticulum had the appearance of a highly-developed network of interconnected tubules, with no lamellar structures. Double impregnation staining combined with high resolution radioautography after [3H]leucine administration showed that the tubular configuration of the endoplasmic reticulum was the main active site of protein synthesis by contrast with the lamellar components, whose activity seemed poor. In terms of protein synthesis, the three-dimensional configuration of the peripheral endoplasmic reticulum of the supraoptic neurons appeared therefore to be closely connected with their functional state."}
{"id": "PMID:466411", "title": "Influence of carotid and aortic baroreceptors on neurosecretory neurons in supraoptic nuclei.", "content": "(1) The effects of arterial baroreceptor stimulation on the activity of 'identified' neurosecretory cells in supraoptic nuclei (SON) of the hypothalamus were investigated in anesthetized cats. (2) Stimulation of baroreceptors by distension of an 'isolated' carotid sinus greatly inhibited SON activity. A linear relationship was found to exist between stimulus intensity (an increase in the sinus pressure) and the degree of inhibition of SON neuron activity. This inhibitory effect was abolished by section of the sinus nerve. (3) The inhibitory effect of baroreceptors on SON neuron activity was most pronounced in the first 5 sec during stimulation, and the effect became less when the stimulus lasted for a long period. At the end of stimulation there was a transient reversal of the response. (4) The excitation of aortic baroreceptors by occluding descending aorta strongly inhibited SON neuron activity. (5) Occlusion of carotid arteries augmented the SON neuron activity. Electrical stimulation of the sinus nerve caused an excitation or an inhibition of SON neurons, depending on the stimulus intensities. Apparently these stimuli produced different degrees of excitation in baro- and chemoreceptors. (6) Combination of chemo- and baroreceptor stimulations revealed that the excitatory effect exerted by chemoreceptor stimulation on SON neurons was reversed or blocked by baroreceptor activation, indicating the powerful inhibitory influence of baroreceptors.", "contents": "Influence of carotid and aortic baroreceptors on neurosecretory neurons in supraoptic nuclei. (1) The effects of arterial baroreceptor stimulation on the activity of 'identified' neurosecretory cells in supraoptic nuclei (SON) of the hypothalamus were investigated in anesthetized cats. (2) Stimulation of baroreceptors by distension of an 'isolated' carotid sinus greatly inhibited SON activity. A linear relationship was found to exist between stimulus intensity (an increase in the sinus pressure) and the degree of inhibition of SON neuron activity. This inhibitory effect was abolished by section of the sinus nerve. (3) The inhibitory effect of baroreceptors on SON neuron activity was most pronounced in the first 5 sec during stimulation, and the effect became less when the stimulus lasted for a long period. At the end of stimulation there was a transient reversal of the response. (4) The excitation of aortic baroreceptors by occluding descending aorta strongly inhibited SON neuron activity. (5) Occlusion of carotid arteries augmented the SON neuron activity. Electrical stimulation of the sinus nerve caused an excitation or an inhibition of SON neurons, depending on the stimulus intensities. Apparently these stimuli produced different degrees of excitation in baro- and chemoreceptors. (6) Combination of chemo- and baroreceptor stimulations revealed that the excitatory effect exerted by chemoreceptor stimulation on SON neurons was reversed or blocked by baroreceptor activation, indicating the powerful inhibitory influence of baroreceptors."}
{"id": "PMID:466412", "title": "GABA-accumulating neurons in the nucleus raphe dorsalis and periaqueductal gray in the rat: a biochemical and radioautographic study.", "content": "The possibility of a GABAergic innervation of the nucleus raphe dorsalis (NRD) has been investigated by using the following approaches: (i) the identification of the principal neuronal groups afferent to the NDR by using horseradish peroxidase retrograde transport, (ii) the determination of glutamate decarboxylase activity (GAD) in the NRD after lesioning these groups or their putative pathways, and (iii) the radioautographic identification of terminals axons and nerve cells accumulating intraventricularly injected [3H]GABA. The hypothesis of a local GABAergic network is supported by the failure to obtain important changes in GAD after lesions of NRD afferents and the presence in this nucleus of terminals, fibers and nerve cell bodies  accumulating [3H]GABA. It appears that these GABA-accumulating neurons could represent a portion of aperiventricular GABAergic system in the periaqueductal gray and the pontine ventricular gray.", "contents": "GABA-accumulating neurons in the nucleus raphe dorsalis and periaqueductal gray in the rat: a biochemical and radioautographic study. The possibility of a GABAergic innervation of the nucleus raphe dorsalis (NRD) has been investigated by using the following approaches: (i) the identification of the principal neuronal groups afferent to the NDR by using horseradish peroxidase retrograde transport, (ii) the determination of glutamate decarboxylase activity (GAD) in the NRD after lesioning these groups or their putative pathways, and (iii) the radioautographic identification of terminals axons and nerve cells accumulating intraventricularly injected [3H]GABA. The hypothesis of a local GABAergic network is supported by the failure to obtain important changes in GAD after lesions of NRD afferents and the presence in this nucleus of terminals, fibers and nerve cell bodies  accumulating [3H]GABA. It appears that these GABA-accumulating neurons could represent a portion of aperiventricular GABAergic system in the periaqueductal gray and the pontine ventricular gray."}
{"id": "PMID:466413", "title": "Brain sodium uptake after choroid plexectomy.", "content": "When in a (control) series of rats brain sodium uptake (BSU) -- as calculated from estimated radioactivities of brain and blood following the injection of [24Na]sodium chloride 30 min and [22Na]sodium chloride one minute before killing -- was plotted against average arterial blood pressure of the animals during the 30 min survival period, a linear dependence of BSU on blood pressure was observed. In another series of animals, in which the choroid plexuses of the fourth and both lateral ventricles had been removed, the regression line showed the same slope, but a significantly lower intercept. On the other hand, a series of rats which had been subjected to a sham operation without removal of the choroid plexuses, showed a regression line not different from the control series. The results indicate that probably the blood pressure independent component of brain sodium uptake, as represented by the intercept of the regression line, for the greater part constitutes cerebrospinal fluid secretion by the choroid plexus.", "contents": "Brain sodium uptake after choroid plexectomy. When in a (control) series of rats brain sodium uptake (BSU) -- as calculated from estimated radioactivities of brain and blood following the injection of [24Na]sodium chloride 30 min and [22Na]sodium chloride one minute before killing -- was plotted against average arterial blood pressure of the animals during the 30 min survival period, a linear dependence of BSU on blood pressure was observed. In another series of animals, in which the choroid plexuses of the fourth and both lateral ventricles had been removed, the regression line showed the same slope, but a significantly lower intercept. On the other hand, a series of rats which had been subjected to a sham operation without removal of the choroid plexuses, showed a regression line not different from the control series. The results indicate that probably the blood pressure independent component of brain sodium uptake, as represented by the intercept of the regression line, for the greater part constitutes cerebrospinal fluid secretion by the choroid plexus."}
{"id": "PMID:466420", "title": "Projections to the neostriatum from the cat precruciate cortex. Anatomy and physiology.", "content": "The projections to the striatum from two cytoarchitectonically and functionally distinct subdivisions of the cat precruciate motor cortex were studied using anatomical and electrophysiological techniques. Our results indicate that the medial precruciate cortex (stimulation of which leads to movements of the axial and proximal musculature) has a widespread projection to the lateral half of the caudate nucleus. The lateral precruciate cortex (stimulation of which leads to movements of the distal musculature) has a localized projection within the caudate nucleus adjacent to the internal capsule. Both medial and lateral precruciate areas project to the putamen. These results are discussed in relation to recent studies suggesting that the basal ganglia are involved in the enabling and sequencing of movements.", "contents": "Projections to the neostriatum from the cat precruciate cortex. Anatomy and physiology. The projections to the striatum from two cytoarchitectonically and functionally distinct subdivisions of the cat precruciate motor cortex were studied using anatomical and electrophysiological techniques. Our results indicate that the medial precruciate cortex (stimulation of which leads to movements of the axial and proximal musculature) has a widespread projection to the lateral half of the caudate nucleus. The lateral precruciate cortex (stimulation of which leads to movements of the distal musculature) has a localized projection within the caudate nucleus adjacent to the internal capsule. Both medial and lateral precruciate areas project to the putamen. These results are discussed in relation to recent studies suggesting that the basal ganglia are involved in the enabling and sequencing of movements."}
{"id": "PMID:466421", "title": "Functional reinnervation of rat hippocampus by locus coeruleus implants.", "content": "Transplants of the embryonic locus coeruleus (LC) region were implanted into the circuity of the hippocampal formation in adult rats in which the normal adrenergic afferents to the hippocampus had been removed. The growth of new adrenergic axons from the implant in the denervated hippocampus was followed for 1-14 months after surgery by means of fluorescence histochemistry, and the function of the implant-hippocampal connections was tested electrophysiologically after 2-3 months survival. In the successful cases the entire hippocampal formation was reinnervated from the LC implant within 3-6 months after operation, and the newly formed innervation still persisted unchanged by 14 months. The reinnervation was equally effective irrespective of the route by which the axons entered the hippocampus, i.e. along the lesioned fornix-fimbria or along a retrosplenial route. The pattern formed by the ingrowing LC axons mimicked to a large extent that of the normal LC afferents. Little growth was seen into denervated terminal fields of the commissural, septal or entorhinal afferents, pointing to a preference of the ingrowing LC fibers for the areas normally innervated by adrenergic afferents. In the electrophysiological experiments, stimulation of the LC implants caused (in 20 out of 29 cells monitored) an inhibition of the spontaneous activity of neurons in the host hippocampus. This inhibition had a relatively long latency and a long duration, similar to that observed after stimulation of the innate LC in the intact rat. As in the normal rat, the inhibitory responses were blocked by systemic or local application of the beta-adrenergic receptor blockers propranolol or sotalol. It is concluded that the adult rat brain is capable of carrying out all steps involved in correct functional reinnervation of a denervated region. Moreover, the implant-hippocampal preparation should be a highly suitable model system for functional studies of a central noradrenergic connection.", "contents": "Functional reinnervation of rat hippocampus by locus coeruleus implants. Transplants of the embryonic locus coeruleus (LC) region were implanted into the circuity of the hippocampal formation in adult rats in which the normal adrenergic afferents to the hippocampus had been removed. The growth of new adrenergic axons from the implant in the denervated hippocampus was followed for 1-14 months after surgery by means of fluorescence histochemistry, and the function of the implant-hippocampal connections was tested electrophysiologically after 2-3 months survival. In the successful cases the entire hippocampal formation was reinnervated from the LC implant within 3-6 months after operation, and the newly formed innervation still persisted unchanged by 14 months. The reinnervation was equally effective irrespective of the route by which the axons entered the hippocampus, i.e. along the lesioned fornix-fimbria or along a retrosplenial route. The pattern formed by the ingrowing LC axons mimicked to a large extent that of the normal LC afferents. Little growth was seen into denervated terminal fields of the commissural, septal or entorhinal afferents, pointing to a preference of the ingrowing LC fibers for the areas normally innervated by adrenergic afferents. In the electrophysiological experiments, stimulation of the LC implants caused (in 20 out of 29 cells monitored) an inhibition of the spontaneous activity of neurons in the host hippocampus. This inhibition had a relatively long latency and a long duration, similar to that observed after stimulation of the innate LC in the intact rat. As in the normal rat, the inhibitory responses were blocked by systemic or local application of the beta-adrenergic receptor blockers propranolol or sotalol. It is concluded that the adult rat brain is capable of carrying out all steps involved in correct functional reinnervation of a denervated region. Moreover, the implant-hippocampal preparation should be a highly suitable model system for functional studies of a central noradrenergic connection."}
{"id": "PMID:466422", "title": "The specificity of reactive synaptogenesis: a comparative study in the adult rat hippocampal formation.", "content": "The CA1 region of the hippocampus in the mature rat is shown to possess the capacity to form new synapses following a lesion of either the commissural afferents, which removes 41% of the synaptic input to stratum radiatum, or commissural and associational afferents, which destroys 74% of the synaptic input. With both types of lesion, extensive reinnervation occurs without obvious changes in lamination of afferent fibers and without accompanying changes in the acetylcholinesterase-(AChE) staining pattern. This is in contrast to what is known to occur in the hippocampal dentate gyrus following an ipsilateral entorhinal lesion where afferent lamination is reordered and where AChE-staining intensifies. A comparison between the disparate patterns of reinnervation in these closely related structures affords the opportunity to examine some of the specific factors that may regulate synaptic readjustments in brain.", "contents": "The specificity of reactive synaptogenesis: a comparative study in the adult rat hippocampal formation. The CA1 region of the hippocampus in the mature rat is shown to possess the capacity to form new synapses following a lesion of either the commissural afferents, which removes 41% of the synaptic input to stratum radiatum, or commissural and associational afferents, which destroys 74% of the synaptic input. With both types of lesion, extensive reinnervation occurs without obvious changes in lamination of afferent fibers and without accompanying changes in the acetylcholinesterase-(AChE) staining pattern. This is in contrast to what is known to occur in the hippocampal dentate gyrus following an ipsilateral entorhinal lesion where afferent lamination is reordered and where AChE-staining intensifies. A comparison between the disparate patterns of reinnervation in these closely related structures affords the opportunity to examine some of the specific factors that may regulate synaptic readjustments in brain."}
{"id": "PMID:466423", "title": "Morphological identification and biochemical characterization of isolated brain cell nuclei from the developing rat cerebellum.", "content": "Cell nuclei from developing rat cerebellum were isolated and the various types of nuclei were characterized and quantified. Nuclear pellets appeared to be both quantitatively and qualitatively representative of the entire cerebellum, and of sufficient purity to perform biochemical studies as well as morphological comparison with histological sections. Isolated nuclei were classified into 6 groups based on nuclear size and shape, heterochromatin aggregations, and nucleoplasmic density. The total population of cerebellar cells primarily consisted of two types of nuclei after day 10. One group of nuclei, resembling those of internal granule neurons or external germinal cells, contributed at least 70% of the total isolated cell nuclei from day 1 to day 90, whereas another nuclear group that was identified as dark oligodendrocytes constituted 8-9% of the total population on days 45 and 90. Nuclear DNA, RNA, and protein content of the cerebellum also were determined throughout postnatal development. DNA concentration markedly declined after day 15, while the RNA/DNA ratio increased until day 3 and remained constant to day 90. The nuclear protein/DNA ratio increased from birth to day 3, decreased to its lowest value on day 10, and increased to day 90. Utilizing DNA values, the total cell population as well as contributions of different cell types were calculated. At birth the cerebellum was estimated to contain 5.9 million cells, increasing to 94 million by day 21. By day 90, 107 million cells were present, of which 8.6 million oligodendrocytes and 93.6 million internal granule cells were estimated.", "contents": "Morphological identification and biochemical characterization of isolated brain cell nuclei from the developing rat cerebellum. Cell nuclei from developing rat cerebellum were isolated and the various types of nuclei were characterized and quantified. Nuclear pellets appeared to be both quantitatively and qualitatively representative of the entire cerebellum, and of sufficient purity to perform biochemical studies as well as morphological comparison with histological sections. Isolated nuclei were classified into 6 groups based on nuclear size and shape, heterochromatin aggregations, and nucleoplasmic density. The total population of cerebellar cells primarily consisted of two types of nuclei after day 10. One group of nuclei, resembling those of internal granule neurons or external germinal cells, contributed at least 70% of the total isolated cell nuclei from day 1 to day 90, whereas another nuclear group that was identified as dark oligodendrocytes constituted 8-9% of the total population on days 45 and 90. Nuclear DNA, RNA, and protein content of the cerebellum also were determined throughout postnatal development. DNA concentration markedly declined after day 15, while the RNA/DNA ratio increased until day 3 and remained constant to day 90. The nuclear protein/DNA ratio increased from birth to day 3, decreased to its lowest value on day 10, and increased to day 90. Utilizing DNA values, the total cell population as well as contributions of different cell types were calculated. At birth the cerebellum was estimated to contain 5.9 million cells, increasing to 94 million by day 21. By day 90, 107 million cells were present, of which 8.6 million oligodendrocytes and 93.6 million internal granule cells were estimated."}
{"id": "PMID:466424", "title": "Central gray and medial hypothalamic stimulation: correlation between escape behavior and unit activity.", "content": "A chronic experiment and an acute one were carried out in the same rat in order to get information on the neuronal events correlated with the escape latency (EL) induced by an electrical stimulation in the mesencephalic central gray (CG) or in the medial hypothalamus (MH). Escape latencies as well as unitary MH and CG neuronal responses (104 units in 25 rats) were studied as functions of the intensity and the pulse duration of a 50 pulses/sec stimulation train. The CG and/or the MH neuronal firing rate was either synchronized or diffusely altered, either inhibited or activated, by the CG or the MH stimulations. Many neuronal firing rate alterations were highly correlated with the escape speed (ES = 1/EL) induced by the same stimulation. Intensity duration trade-off functions were computed from both the EL and the unit recordings. Chronaxie determinations were performed from these data: the behaviorally determined chronaxies did not differ from the unitary ones (limit values: 0.05-0.42 msec). The discussion bear on the possible role of the observed neuronal alterations induced by CG or MH stimulations in relation to their behavioral effects.", "contents": "Central gray and medial hypothalamic stimulation: correlation between escape behavior and unit activity. A chronic experiment and an acute one were carried out in the same rat in order to get information on the neuronal events correlated with the escape latency (EL) induced by an electrical stimulation in the mesencephalic central gray (CG) or in the medial hypothalamus (MH). Escape latencies as well as unitary MH and CG neuronal responses (104 units in 25 rats) were studied as functions of the intensity and the pulse duration of a 50 pulses/sec stimulation train. The CG and/or the MH neuronal firing rate was either synchronized or diffusely altered, either inhibited or activated, by the CG or the MH stimulations. Many neuronal firing rate alterations were highly correlated with the escape speed (ES = 1/EL) induced by the same stimulation. Intensity duration trade-off functions were computed from both the EL and the unit recordings. Chronaxie determinations were performed from these data: the behaviorally determined chronaxies did not differ from the unitary ones (limit values: 0.05-0.42 msec). The discussion bear on the possible role of the observed neuronal alterations induced by CG or MH stimulations in relation to their behavioral effects."}
{"id": "PMID:466425", "title": "Catecholamines and convulsions.", "content": "Severe depletion of brain noradrenaline and separately of brain dopamine was induced in rats by intracerebral injection of the selective neurotoxin 6-hydroxydopamine, and the susceptibility of the treated animals to various seizure-inducing manipulations was examined. A significant potentiation of the seizures induced both by Metrazol and by electroconvulsive shock was found in animals depleted of brain noradrenaline, but no alteration was seen after depletion of brain dopamine on either measure. The catecholaminergic drug cocaine also induced seizures, but these were found not to depend on either brain noradrenaline or dopamine as they continued to occur in the virtual absence of either catecholamine. It is concluded that cocaine induces seizures by a non-specific toxic mechanism and that noradrenaline, but not dopamine, is involved in reducing the suceptibility of the central nervous system to the several distinct forms of seizure induction examined.", "contents": "Catecholamines and convulsions. Severe depletion of brain noradrenaline and separately of brain dopamine was induced in rats by intracerebral injection of the selective neurotoxin 6-hydroxydopamine, and the susceptibility of the treated animals to various seizure-inducing manipulations was examined. A significant potentiation of the seizures induced both by Metrazol and by electroconvulsive shock was found in animals depleted of brain noradrenaline, but no alteration was seen after depletion of brain dopamine on either measure. The catecholaminergic drug cocaine also induced seizures, but these were found not to depend on either brain noradrenaline or dopamine as they continued to occur in the virtual absence of either catecholamine. It is concluded that cocaine induces seizures by a non-specific toxic mechanism and that noradrenaline, but not dopamine, is involved in reducing the suceptibility of the central nervous system to the several distinct forms of seizure induction examined."}
{"id": "PMID:466426", "title": "The effect of hyperglycemia on brain capillary permeability in the lizard, Anolis carolinensis. A freeze-fracture analysis of blood-brain barrier pathology.", "content": "The anatomical basis of the blood-brain barrier in the American chameleon, Anolis carolinensis, is the system of tight intercellular junctions that occurs between apposed endothelial cells of brain capillaries. Under normal physiological conditions, capillaries in the brain cortex of these animals remain sealed by interendothelial zonulae occludentes and, consequently, escape of exogenous tracer proteins such as horseradish peroxidase (HRP) into the extracellular compartment of the central nervous system is prevented. Systemic injection of 2.7 mg of D-glucose into chameleons results in increased brain capillary permeability, as evidenced by escape of HRP or Trypan blue into the intercellular spaces of central neuropil. Freeze-fracture analysis of brain capillary endothelia of glucose-hyperglycemic lizards revealed no alteration of the ridge and groove construction of endothelial tight junctions, indicating that although the blood-brain interface becomes leaky during severe hyperglycemia, the capillary zonulae occludentes are not affected. Evidence obtained in this study strongly supports the notion that the increased capillary permeability is the result of amplified transendothelial transport. The effect is manifest as and facilitated by the formation of chains of pinocytotic vesicles derived from the luminal surface of the endothelial cells, which fuse to create open trans-endothelial conduits. It is likely that formation of open channels that traverse brain capillary endothelial cells, as a response to hyperglycemia, could allow temporarily unrestricted passage of a wide range of molecules, some potentially toxic, into the CNS extracellular milieu. This is the first report to unequivocally document with freeze-cleave techniques, that abnormally elevated levels of blood sugar can affect blood-brain interface permeability. This finding suggests that similar consequences may be expected to result from diabetic hyperglycemia in humans.", "contents": "The effect of hyperglycemia on brain capillary permeability in the lizard, Anolis carolinensis. A freeze-fracture analysis of blood-brain barrier pathology. The anatomical basis of the blood-brain barrier in the American chameleon, Anolis carolinensis, is the system of tight intercellular junctions that occurs between apposed endothelial cells of brain capillaries. Under normal physiological conditions, capillaries in the brain cortex of these animals remain sealed by interendothelial zonulae occludentes and, consequently, escape of exogenous tracer proteins such as horseradish peroxidase (HRP) into the extracellular compartment of the central nervous system is prevented. Systemic injection of 2.7 mg of D-glucose into chameleons results in increased brain capillary permeability, as evidenced by escape of HRP or Trypan blue into the intercellular spaces of central neuropil. Freeze-fracture analysis of brain capillary endothelia of glucose-hyperglycemic lizards revealed no alteration of the ridge and groove construction of endothelial tight junctions, indicating that although the blood-brain interface becomes leaky during severe hyperglycemia, the capillary zonulae occludentes are not affected. Evidence obtained in this study strongly supports the notion that the increased capillary permeability is the result of amplified transendothelial transport. The effect is manifest as and facilitated by the formation of chains of pinocytotic vesicles derived from the luminal surface of the endothelial cells, which fuse to create open trans-endothelial conduits. It is likely that formation of open channels that traverse brain capillary endothelial cells, as a response to hyperglycemia, could allow temporarily unrestricted passage of a wide range of molecules, some potentially toxic, into the CNS extracellular milieu. This is the first report to unequivocally document with freeze-cleave techniques, that abnormally elevated levels of blood sugar can affect blood-brain interface permeability. This finding suggests that similar consequences may be expected to result from diabetic hyperglycemia in humans."}
{"id": "PMID:466427", "title": "Conditioned aversion to brain-stimulation reward: effects of electrode placement and prior experience.", "content": "A conditioned aversion to rewarding amygdaloid brain-stimulation was established by injecting rats with toxic doses of 0.15 M LiCl immediately after an initial self-stimulation session. The aversion had extinguished by the third self-stimulation session, 96 h after conditioning. This effect cannot be attributed to general depressant effects of LiCl on self-stimulation as treatment with LiCl 24 h before the second self-stimulation session was ineffective. Furthermore, this conditioned aversion was locus specific as LiCl injections immediately after the first test session had no disruptive effects on self-stimulation in the substantia nigra. The parallels between conditioned aversion to rewarding brain-stimulation in the amygdala and late aversion were strengthened by the fact that the novelty of brain-stimulation reward was an important factor in the conditioning effect. These data have important implications for understanding the sensory properties of brain-stimulation reward.", "contents": "Conditioned aversion to brain-stimulation reward: effects of electrode placement and prior experience. A conditioned aversion to rewarding amygdaloid brain-stimulation was established by injecting rats with toxic doses of 0.15 M LiCl immediately after an initial self-stimulation session. The aversion had extinguished by the third self-stimulation session, 96 h after conditioning. This effect cannot be attributed to general depressant effects of LiCl on self-stimulation as treatment with LiCl 24 h before the second self-stimulation session was ineffective. Furthermore, this conditioned aversion was locus specific as LiCl injections immediately after the first test session had no disruptive effects on self-stimulation in the substantia nigra. The parallels between conditioned aversion to rewarding brain-stimulation in the amygdala and late aversion were strengthened by the fact that the novelty of brain-stimulation reward was an important factor in the conditioning effect. These data have important implications for understanding the sensory properties of brain-stimulation reward."}
{"id": "PMID:466431", "title": "Experimental hypertension and catecholamine distribution in the rat brain.", "content": "Hypertension was induced in rats (Hebrew University strain) by three different procedures: (1) deoxycorticosterone acetate (DOCA)--salt treatment; (2) unilateral renal artery clip or (3) chronic salt-loading. Noradrenaline (NA) and dopamine (DA) distribution in different brain areas was assayed following induction of hypertension. NA content increased significantly in various areas: the increase of NA in the pons-medulla was common to all procedures inducing hypertension. NA content increased also in the mesencephalon, the hypothalamus and the rest of the forebrain (DOCA--salt hypertension), in the mesencephalon, the hypothalamus and the cortex (in renal clip hypertension). No significant changes in DA content were observed in any region of the brain following induction of hypertension by the three different methods. In two substrains, selected from the Hebrew University strain, for their respective sensitivity (H) or immunity (N) to hypertension induced by DOCA--salt treatment, there were no significant increases in NA or DA in any part of the brain following DOCA--salt treatment. Comparison of NA concentrations in these strains showed that NA was significantly higher in the pons-medulla of the untreated N strain rats than in the medulla of untreated H strain or in untreated rats of the original strain (Hebrew University). A model is presented suggesting that central NA-containing neurons plays a major role in controlling hypertension.", "contents": "Experimental hypertension and catecholamine distribution in the rat brain. Hypertension was induced in rats (Hebrew University strain) by three different procedures: (1) deoxycorticosterone acetate (DOCA)--salt treatment; (2) unilateral renal artery clip or (3) chronic salt-loading. Noradrenaline (NA) and dopamine (DA) distribution in different brain areas was assayed following induction of hypertension. NA content increased significantly in various areas: the increase of NA in the pons-medulla was common to all procedures inducing hypertension. NA content increased also in the mesencephalon, the hypothalamus and the rest of the forebrain (DOCA--salt hypertension), in the mesencephalon, the hypothalamus and the cortex (in renal clip hypertension). No significant changes in DA content were observed in any region of the brain following induction of hypertension by the three different methods. In two substrains, selected from the Hebrew University strain, for their respective sensitivity (H) or immunity (N) to hypertension induced by DOCA--salt treatment, there were no significant increases in NA or DA in any part of the brain following DOCA--salt treatment. Comparison of NA concentrations in these strains showed that NA was significantly higher in the pons-medulla of the untreated N strain rats than in the medulla of untreated H strain or in untreated rats of the original strain (Hebrew University). A model is presented suggesting that central NA-containing neurons plays a major role in controlling hypertension."}
{"id": "PMID:466432", "title": "Responses of substantia nigra neurones to noxious stimulation.", "content": "Experiments were performed to determine whether substantia nigra neurones respond to noxious stimulation. Some nigral neurones were found to respond to both noxious radiant heat and intra-arterially administered bradykinin. Most nociceptive neurones were excited by noxious stimulation although some exhibited a reduction in firing rate. Following the injection of bradykinin, responses to noxious heat were reduced in magnitude for periods of up to an hour. These results show that some nigral neurones are nociceptive and provide further support for the suggestion that dopamine is involved in nociceptive mechanisms. In addition it is suggested that bradykinin may represent a rather gross of non-specific form of noxious stimulation.", "contents": "Responses of substantia nigra neurones to noxious stimulation. Experiments were performed to determine whether substantia nigra neurones respond to noxious stimulation. Some nigral neurones were found to respond to both noxious radiant heat and intra-arterially administered bradykinin. Most nociceptive neurones were excited by noxious stimulation although some exhibited a reduction in firing rate. Following the injection of bradykinin, responses to noxious heat were reduced in magnitude for periods of up to an hour. These results show that some nigral neurones are nociceptive and provide further support for the suggestion that dopamine is involved in nociceptive mechanisms. In addition it is suggested that bradykinin may represent a rather gross of non-specific form of noxious stimulation."}
{"id": "PMID:466438", "title": "Restoration of corticosteroid periodicity in obese rats by limited a.m. food access.", "content": "Absence of circadian periodicity of plasma corticosteroid levels has previously been reported to occur in animals maintained under constant light and in animals with suprachiasmatic nuclear lesions. Such animals also exhibit aperiodic rather than normal nocturnal eating. Restricted a.m. access to food (09.30--11.30 h) in such animals results in appearance of circadian corticosteroid periodicity (peak at 08.00 h) similar to that seen in normal animals on a similar food restriction regimen. The present study investigated the nature of adrenal circadian periodicity in obese rats who are reported to exhibit aperiodic feeding. Such aperiodic feeding occurred in obese male, but not in obese female, animals. These obese male animals manifested absent corticosteroid circadian periodicity. Restricted a.m. access to food was associated with the appearance of such periodicity similar to that described above in the two other experimental situations. These studies indicate differences in eating behavior and corticosteroid periodicity between obese male and obese female rats. They provide additional confirmation, in animals under normal light--dark conditions and in the absence of CNS lesions, of the role of periodic feeding in the generation of adrenal circadian periodicity.", "contents": "Restoration of corticosteroid periodicity in obese rats by limited a.m. food access. Absence of circadian periodicity of plasma corticosteroid levels has previously been reported to occur in animals maintained under constant light and in animals with suprachiasmatic nuclear lesions. Such animals also exhibit aperiodic rather than normal nocturnal eating. Restricted a.m. access to food (09.30--11.30 h) in such animals results in appearance of circadian corticosteroid periodicity (peak at 08.00 h) similar to that seen in normal animals on a similar food restriction regimen. The present study investigated the nature of adrenal circadian periodicity in obese rats who are reported to exhibit aperiodic feeding. Such aperiodic feeding occurred in obese male, but not in obese female, animals. These obese male animals manifested absent corticosteroid circadian periodicity. Restricted a.m. access to food was associated with the appearance of such periodicity similar to that described above in the two other experimental situations. These studies indicate differences in eating behavior and corticosteroid periodicity between obese male and obese female rats. They provide additional confirmation, in animals under normal light--dark conditions and in the absence of CNS lesions, of the role of periodic feeding in the generation of adrenal circadian periodicity."}
{"id": "PMID:466439", "title": "The discrete anatomical localization of vagal aortic afferents within a catecholamine-containing cell group in the nucleus solitarius.", "content": "The centripetal transport of horseradish peroxidase (HRP) was used to visualize the central projections of afferent fibers of the aortic arch nerve to the nucleus solitarius in pigeons. Our results indicate that aortic nerve afferents have a restricted distribution within one subdivision of the nucleus solitarius, designated the subnucleus sulcalis dorsalis. Histofluorescence studies revealed that the aortic nerve projection area is extremely rich in catecholamine-containing neurons.", "contents": "The discrete anatomical localization of vagal aortic afferents within a catecholamine-containing cell group in the nucleus solitarius. The centripetal transport of horseradish peroxidase (HRP) was used to visualize the central projections of afferent fibers of the aortic arch nerve to the nucleus solitarius in pigeons. Our results indicate that aortic nerve afferents have a restricted distribution within one subdivision of the nucleus solitarius, designated the subnucleus sulcalis dorsalis. Histofluorescence studies revealed that the aortic nerve projection area is extremely rich in catecholamine-containing neurons."}
{"id": "PMID:466440", "title": "Retinal ganglion cell response to axotomy and nerve growth factor in the regenerating visual system of the newt (Notophthalmus viridescens): an ultrastructural morphometric analysis.", "content": "Nerve growth factor (NGF) treatment, given as a single 200 BU intraocular injection at the time of optic nerve transection, was found to significantly accelerate the retinal ganglion cell response to axotomy in the newt (Notophthalmus viridescens). In the control series the per cent of neurons in the retinal ganglion layer demonstrating nuclear reactivity (i.e. chromatin changes) reaches a peak by 14 days post axotomy (14 DPA), plateaus through 21 DPA and falls thereafter, returning to control levels by 90 DPA. NGF treatment is shown to significantly accelerate the entrance of responding retinal ganglion cells into the reactive nuclear phase between 1 and 7 DPA, and by 7 DPA nuclear reactivity has reached a peak, in contrast to 14 DPA for control values. Consequently, NGF treatment causes retinal ganglion cells to be in the nuclear reactive state a week longer than controls but reactivity diminishes after 21 DPA as in controls. Electron microscopic morphometric analysis further substantiates these observations by demonstrating that NGF treatment can elicit certain cellular organelle changes a week earlier (i.e. at 7 DPA) than they would normally occur (i.e. at 14 DPA) in response to axotomy. In addition to eliciting cellular hypertrophy at 7 DPA, NGF treatment significantly increases Golgi field densities in the neuronal perikaryal cytoplasm as well as a doubling of the number of nucleoli per nucleus and stimulating a significant increase in nucleolar cross-sectional areas. A dose-response relationship exists between the per cent of retinal ganglion cells demonstrating nuclear reactivity at 7 DPA and various NGF concentrations which compares favorably with the dose response study involving the number of regenerating axons per nerve cross-section at 14 DPA. Studies to determine if the NGF mediated responses were a specific effect elicited by this protein molecule or whether they are also produced by other peptides which share some properties in common with NGF demonstrate that only NGF is capable of eliciting these responses.", "contents": "Retinal ganglion cell response to axotomy and nerve growth factor in the regenerating visual system of the newt (Notophthalmus viridescens): an ultrastructural morphometric analysis. Nerve growth factor (NGF) treatment, given as a single 200 BU intraocular injection at the time of optic nerve transection, was found to significantly accelerate the retinal ganglion cell response to axotomy in the newt (Notophthalmus viridescens). In the control series the per cent of neurons in the retinal ganglion layer demonstrating nuclear reactivity (i.e. chromatin changes) reaches a peak by 14 days post axotomy (14 DPA), plateaus through 21 DPA and falls thereafter, returning to control levels by 90 DPA. NGF treatment is shown to significantly accelerate the entrance of responding retinal ganglion cells into the reactive nuclear phase between 1 and 7 DPA, and by 7 DPA nuclear reactivity has reached a peak, in contrast to 14 DPA for control values. Consequently, NGF treatment causes retinal ganglion cells to be in the nuclear reactive state a week longer than controls but reactivity diminishes after 21 DPA as in controls. Electron microscopic morphometric analysis further substantiates these observations by demonstrating that NGF treatment can elicit certain cellular organelle changes a week earlier (i.e. at 7 DPA) than they would normally occur (i.e. at 14 DPA) in response to axotomy. In addition to eliciting cellular hypertrophy at 7 DPA, NGF treatment significantly increases Golgi field densities in the neuronal perikaryal cytoplasm as well as a doubling of the number of nucleoli per nucleus and stimulating a significant increase in nucleolar cross-sectional areas. A dose-response relationship exists between the per cent of retinal ganglion cells demonstrating nuclear reactivity at 7 DPA and various NGF concentrations which compares favorably with the dose response study involving the number of regenerating axons per nerve cross-section at 14 DPA. Studies to determine if the NGF mediated responses were a specific effect elicited by this protein molecule or whether they are also produced by other peptides which share some properties in common with NGF demonstrate that only NGF is capable of eliciting these responses."}
{"id": "PMID:466441", "title": "Neurochemical and morphological studies of bulk isolated rat brain cells. II. Preparation of viable cerebral neurons which retain synaptic complexes.", "content": "The bulk isolation from rat cerebral cortex of viable neurons retaining synaptic complexes is described. The basis of this procedure is to dissociate the neurons in situ from the surrounding glial cells. The glial structures that are normally adjacent to the neuronal cell body and to the proximal parts of the neuronal processes are largely destroyed by perfusion of the brain under special conditions. The most important of these conditions was found to be a hyperosmolar concentration of hexoses in the perfusion medium. In addition, the presence of collagenase and hyaluronidase in the perfusion medium and specific perfusate flow characteristics were required to produce the structural changes throughout the brain tissue. When the perfused brain was further dissociated into a cell suspension by mincing and sieving, isolated neurons were obtained, the majority of which retained the proximal parts of their processes. A novel feature of these neurons was the retention of synaptic boutons on the plasma membrane. Presynaptic terminals with mitochondria and vesicles as well as pre- and postsynaptic membranes and densities were observed on the isolated neurons. The neurons were fractionated to 90--95% purity using discontinuous Ficoll density gradient centrifugation with a liquid fluorocarbon as cushion. Highly purified, viable cerebral neurons retaining synaptic complexes are thus available in bulk for neurobiological studies.", "contents": "Neurochemical and morphological studies of bulk isolated rat brain cells. II. Preparation of viable cerebral neurons which retain synaptic complexes. The bulk isolation from rat cerebral cortex of viable neurons retaining synaptic complexes is described. The basis of this procedure is to dissociate the neurons in situ from the surrounding glial cells. The glial structures that are normally adjacent to the neuronal cell body and to the proximal parts of the neuronal processes are largely destroyed by perfusion of the brain under special conditions. The most important of these conditions was found to be a hyperosmolar concentration of hexoses in the perfusion medium. In addition, the presence of collagenase and hyaluronidase in the perfusion medium and specific perfusate flow characteristics were required to produce the structural changes throughout the brain tissue. When the perfused brain was further dissociated into a cell suspension by mincing and sieving, isolated neurons were obtained, the majority of which retained the proximal parts of their processes. A novel feature of these neurons was the retention of synaptic boutons on the plasma membrane. Presynaptic terminals with mitochondria and vesicles as well as pre- and postsynaptic membranes and densities were observed on the isolated neurons. The neurons were fractionated to 90--95% purity using discontinuous Ficoll density gradient centrifugation with a liquid fluorocarbon as cushion. Highly purified, viable cerebral neurons retaining synaptic complexes are thus available in bulk for neurobiological studies."}
{"id": "PMID:466442", "title": "Neuritogenic and chemical properties of guinea pig anterior and posterior root myelin.", "content": "In relapsing experimental allergic encephalomyelitis, recurrent demyelination was found in the anterior roots and dorsal root ganglia with minimal involvement of the posterior roots. To determine whether this is an antigen-related phenomenon, the distribution, type and intensity of the lesions in the proximal PNS of guinea pigs immunized with anterior roots or myelin were compared to those of animals immunized with posterior roots or myelin. Homologous anterior roots were less neuritogenic than posterior roots or posterior root myelin. Thin layer chromatography of myelin samples from anterior and posterior roots, dorsal root ganglia and sciatic nerve revealed the presence of a sulfogalactoglycerolipid, tentatively identified as sulfated galactosylglyceride (SGG) in all but the posterior root myelin samples. Although the PNS lesions of relapsing experimental allergic encephalomyelitis appear to recapitulate the regional distribution of SGG, the reason why its presence in anterior roots myelin renders them less neuritogenic is at present not clear.", "contents": "Neuritogenic and chemical properties of guinea pig anterior and posterior root myelin. In relapsing experimental allergic encephalomyelitis, recurrent demyelination was found in the anterior roots and dorsal root ganglia with minimal involvement of the posterior roots. To determine whether this is an antigen-related phenomenon, the distribution, type and intensity of the lesions in the proximal PNS of guinea pigs immunized with anterior roots or myelin were compared to those of animals immunized with posterior roots or myelin. Homologous anterior roots were less neuritogenic than posterior roots or posterior root myelin. Thin layer chromatography of myelin samples from anterior and posterior roots, dorsal root ganglia and sciatic nerve revealed the presence of a sulfogalactoglycerolipid, tentatively identified as sulfated galactosylglyceride (SGG) in all but the posterior root myelin samples. Although the PNS lesions of relapsing experimental allergic encephalomyelitis appear to recapitulate the regional distribution of SGG, the reason why its presence in anterior roots myelin renders them less neuritogenic is at present not clear."}
{"id": "PMID:466443", "title": "Regional distribution of methionine-enkephalin- and beta-endorphin-like immunoreactivity in human brain and pituitary.", "content": "Concentrations of methionine-enkephalin- (Met-enkephalin) and beta-endorphin-like immunoreactivities were determined in 33 areas of human brain and pituitary using highly sensitive radioimmunoassays in combination with affinity chromatography for the purification of beta-endorphin. It was found that they have quite different distribution patterns, suggesting the existence of both endorphins in independent systems in the central nervous system. Determination of Met-enkephalin and beta-endorphin immunoreactivities in chronic alcoholics and opiate-dependent subjects revealed no gross changes in comparison to the normal subjects.", "contents": "Regional distribution of methionine-enkephalin- and beta-endorphin-like immunoreactivity in human brain and pituitary. Concentrations of methionine-enkephalin- (Met-enkephalin) and beta-endorphin-like immunoreactivities were determined in 33 areas of human brain and pituitary using highly sensitive radioimmunoassays in combination with affinity chromatography for the purification of beta-endorphin. It was found that they have quite different distribution patterns, suggesting the existence of both endorphins in independent systems in the central nervous system. Determination of Met-enkephalin and beta-endorphin immunoreactivities in chronic alcoholics and opiate-dependent subjects revealed no gross changes in comparison to the normal subjects."}
{"id": "PMID:466444", "title": "Regeneration of normal terminal innervation patterns by central noradrenergic neurons after 5,7-dihydroxytryptamine-induced axotomy in the adult rat.", "content": "The regeneration of central adrenergic axons has been followed between 5 days and 18 months after 5,7-dihydroxytryptamine(5,7-DHT)-induced axotomy in the adult rat, using fluorescence histochemistry in combination with noradrenaline (NA) determinations and [3H]NA uptake measurements. The axonal and terminal degeneration caused by the 5,7-DHT treatment (150 micrograms intraventricularly) was, by 1--2 weeks after injection, accompanied by a 70% reduction of NA in the forebrain and 30% reduction in the brain stem, and by 43--85% reductions in the [3H]NA uptake capacity in various regions of the brain and spinal cord. Signs of sprouting of the drug-lesioned axons were evident along the terminal axon segments at 5 days after treatment. The sprouts increased rapidly in length and number during the subsequent weeks and by 2--6 months after injection new NA terminal systems of relatively normal density and distribution had been re-established in many initially denervated regions. In parallel there was a recovery of endogenous NA and [3H]NA uptake to the pre-injection levels in the brain, and to levels 50--75% of normal in the cervical and thoracic spinal cord. Four successive phases of the regeneration process are distinguished: (1) primary sprouting from the lesioned NA axon stumps, occurring within the first week after treatment; (2) seemingly random growth and proliferation of the newly formed sprouts during the second and third weeks; (3) directed, forward growth of some of the sprouts leading to a partial restoration of the original fibre paths, branching patterns and terminal networks within 3--6 months; (4) a concomitant removal of at least part of the abnormally directed sprouts. Although the original fibre architecture was quite accurately restored in many areas the regeneration was not always correct. Hyperinnervation patterns and abnormal terminal arrangements were often formed, and in the spinal cord the down-growth of the regenerating axons occurred predominantly along a route that is inconspicuous in the normal rat. It is concluded that at least certain types of central neurons regenerate very efficiently provided the conditions are favourable, and that under such conditions axonal regeneration in the mammalian CNS is subjected to regulatory mechanisms that can be very precise. The results provide evidence that the adult mammalian CNS possesses mechanisms for axonal guidance which allow the accurate regeneration of lesioned axonal tracts and branching patterns, as well as mechanisms of recognition making possible the re-establishment of the original terminal connections.", "contents": "Regeneration of normal terminal innervation patterns by central noradrenergic neurons after 5,7-dihydroxytryptamine-induced axotomy in the adult rat. The regeneration of central adrenergic axons has been followed between 5 days and 18 months after 5,7-dihydroxytryptamine(5,7-DHT)-induced axotomy in the adult rat, using fluorescence histochemistry in combination with noradrenaline (NA) determinations and [3H]NA uptake measurements. The axonal and terminal degeneration caused by the 5,7-DHT treatment (150 micrograms intraventricularly) was, by 1--2 weeks after injection, accompanied by a 70% reduction of NA in the forebrain and 30% reduction in the brain stem, and by 43--85% reductions in the [3H]NA uptake capacity in various regions of the brain and spinal cord. Signs of sprouting of the drug-lesioned axons were evident along the terminal axon segments at 5 days after treatment. The sprouts increased rapidly in length and number during the subsequent weeks and by 2--6 months after injection new NA terminal systems of relatively normal density and distribution had been re-established in many initially denervated regions. In parallel there was a recovery of endogenous NA and [3H]NA uptake to the pre-injection levels in the brain, and to levels 50--75% of normal in the cervical and thoracic spinal cord. Four successive phases of the regeneration process are distinguished: (1) primary sprouting from the lesioned NA axon stumps, occurring within the first week after treatment; (2) seemingly random growth and proliferation of the newly formed sprouts during the second and third weeks; (3) directed, forward growth of some of the sprouts leading to a partial restoration of the original fibre paths, branching patterns and terminal networks within 3--6 months; (4) a concomitant removal of at least part of the abnormally directed sprouts. Although the original fibre architecture was quite accurately restored in many areas the regeneration was not always correct. Hyperinnervation patterns and abnormal terminal arrangements were often formed, and in the spinal cord the down-growth of the regenerating axons occurred predominantly along a route that is inconspicuous in the normal rat. It is concluded that at least certain types of central neurons regenerate very efficiently provided the conditions are favourable, and that under such conditions axonal regeneration in the mammalian CNS is subjected to regulatory mechanisms that can be very precise. The results provide evidence that the adult mammalian CNS possesses mechanisms for axonal guidance which allow the accurate regeneration of lesioned axonal tracts and branching patterns, as well as mechanisms of recognition making possible the re-establishment of the original terminal connections."}
{"id": "PMID:466445", "title": "The effects of acute morphine treatment on the incorporation of [3H]L-lysine by normal and regenerating facial nucleus neurons.", "content": "Although opiates significantly alter RNA and protein synthesis in a variety of neuronal cell types, their effect on the biosynthetic activity of regenerating neurons has not been investigated. In the present study, the effect of morphine on the incorporation of [3H]L-lysine into proteins of facial nucleus neurons was examined by light microscopic radioautography. Silver grains present within various compartments of normal and regenerating (3-, 7-, 14- and 21 days post-axotomy) neurons from saline-treated Wistar rats were compared with the amount present in similar cells from animals receiving 40 mg/kg morphine sulfate i.v. At 14- and 21-days post-axotomy, regenerating neurons were larger and the grain count in the emulsion over these cells was greater than that observed in normal (unoperated) neurons. In normal facial neurons, the accumulation of lysine into the nucleus and nucleolus was significantly lower 60 min after morphine administration. However, morphine's inhibition of lysine incorporation was even more pronounced in regenerating neurons. In these cells, nuclear lysine uptake was depressed at 3 and 7 days, while maximum inhibition of cytoplasmic incorporation occurred at 14-days post-axotomy. Morphine administration decreased nucleolar lysine incorporation at all survival intervals.", "contents": "The effects of acute morphine treatment on the incorporation of [3H]L-lysine by normal and regenerating facial nucleus neurons. Although opiates significantly alter RNA and protein synthesis in a variety of neuronal cell types, their effect on the biosynthetic activity of regenerating neurons has not been investigated. In the present study, the effect of morphine on the incorporation of [3H]L-lysine into proteins of facial nucleus neurons was examined by light microscopic radioautography. Silver grains present within various compartments of normal and regenerating (3-, 7-, 14- and 21 days post-axotomy) neurons from saline-treated Wistar rats were compared with the amount present in similar cells from animals receiving 40 mg/kg morphine sulfate i.v. At 14- and 21-days post-axotomy, regenerating neurons were larger and the grain count in the emulsion over these cells was greater than that observed in normal (unoperated) neurons. In normal facial neurons, the accumulation of lysine into the nucleus and nucleolus was significantly lower 60 min after morphine administration. However, morphine's inhibition of lysine incorporation was even more pronounced in regenerating neurons. In these cells, nuclear lysine uptake was depressed at 3 and 7 days, while maximum inhibition of cytoplasmic incorporation occurred at 14-days post-axotomy. Morphine administration decreased nucleolar lysine incorporation at all survival intervals."}
{"id": "PMID:466453", "title": "Regional variation and absence of large neurons in the cerebellum of the staggerer mouse.", "content": "Staggerer (sg) is a neurological mutant mouse in which the cerebellar granule cells degenerate after migration to the internal granule cell layer. In addition, the Purkinje cells are abnormal, being ectopic, smaller in size and without tertiary branchlet spines. In this study we report two new observations on this mutant: (1) the effects of the mutation show a regional variation in severity along the mediolateral axis. This variation is seen in the cross-sectional size of the tissue, the extent of cortical folding, as well as the density and cytological appearance of the medium-to-large cortical neurons (MLNs); (2) cell counts were done of 30-day-old mutants and of wild-type controls. The counts revealed that three-quarters of the MLNs of the cerebellar cortex are missing in staggerer. These findings cannot exclude the possibility that Golgi as well as Purkinje cells are absent since the two cannot be distinguished in staggerer. Depending on the number of Golgi cells present, between 60% and 90% of the wild-type number of Purkinje cells is missing in staggerer. The results are discussed in terms of their implications for other studies and for locating the site of staggerer gene action.", "contents": "Regional variation and absence of large neurons in the cerebellum of the staggerer mouse. Staggerer (sg) is a neurological mutant mouse in which the cerebellar granule cells degenerate after migration to the internal granule cell layer. In addition, the Purkinje cells are abnormal, being ectopic, smaller in size and without tertiary branchlet spines. In this study we report two new observations on this mutant: (1) the effects of the mutation show a regional variation in severity along the mediolateral axis. This variation is seen in the cross-sectional size of the tissue, the extent of cortical folding, as well as the density and cytological appearance of the medium-to-large cortical neurons (MLNs); (2) cell counts were done of 30-day-old mutants and of wild-type controls. The counts revealed that three-quarters of the MLNs of the cerebellar cortex are missing in staggerer. These findings cannot exclude the possibility that Golgi as well as Purkinje cells are absent since the two cannot be distinguished in staggerer. Depending on the number of Golgi cells present, between 60% and 90% of the wild-type number of Purkinje cells is missing in staggerer. The results are discussed in terms of their implications for other studies and for locating the site of staggerer gene action."}
{"id": "PMID:466454", "title": "Mapping study of brain dopamine- and epinephrine-sensitive sites which cause feeding suppression in the rat.", "content": "Central injections of dopamine (DA) or epinephrine (EPI) have been found to suppress feeding behavior in hungry rats. In the present study, 24 different brain areas, in 299 animals, were examined to localize the precise region of catecholamine (CA) sensitivity. Essentially all sites outside the hypothalamus, as well as in the medial portion of the hypothalamus, were relatively or totally unresponsive to DA or EPI. The area of greatest sensitivity for both agonists (where they yielded a 50--70% suppression of feeding) was found to be the perifornical region of the lateral hypothalamus, extending from the caudal aspect of the paraventricular nucleus to the caudal aspect of the ventromedial nucleus. Dorsal, lateral, or ventrolateral movement of the injection site away from the fornix and into the zona incerta or the lateral hypothalamic medial forebrain bundle area caused a dramatic reduction in the effectiveness of the CA. These findings are consistent with histochemical studies, which have shown the fornix to be surrounded by CA varicosities, and pharmacological studies, which have shown the perifornical region to be most sensitive to the anorexic effect of centrally injected amphetamine, which releases endogenous CA. It is suggested that the perifornical hypothalamus plays a role in the process of inhibiting food consumption in response to increased dopaminergic and adrenergic activity.", "contents": "Mapping study of brain dopamine- and epinephrine-sensitive sites which cause feeding suppression in the rat. Central injections of dopamine (DA) or epinephrine (EPI) have been found to suppress feeding behavior in hungry rats. In the present study, 24 different brain areas, in 299 animals, were examined to localize the precise region of catecholamine (CA) sensitivity. Essentially all sites outside the hypothalamus, as well as in the medial portion of the hypothalamus, were relatively or totally unresponsive to DA or EPI. The area of greatest sensitivity for both agonists (where they yielded a 50--70% suppression of feeding) was found to be the perifornical region of the lateral hypothalamus, extending from the caudal aspect of the paraventricular nucleus to the caudal aspect of the ventromedial nucleus. Dorsal, lateral, or ventrolateral movement of the injection site away from the fornix and into the zona incerta or the lateral hypothalamic medial forebrain bundle area caused a dramatic reduction in the effectiveness of the CA. These findings are consistent with histochemical studies, which have shown the fornix to be surrounded by CA varicosities, and pharmacological studies, which have shown the perifornical region to be most sensitive to the anorexic effect of centrally injected amphetamine, which releases endogenous CA. It is suggested that the perifornical hypothalamus plays a role in the process of inhibiting food consumption in response to increased dopaminergic and adrenergic activity."}
{"id": "PMID:466456", "title": "Taurine effects on 45Ca2+ transport in retinal subcellular fractions.", "content": "The effect of taurine on 45Ca2+ transport by subcellular fractions from the chick retina was examined. An inhibitory action of taurine on 45Ca2+ uptake was observed in retinal fractions incubated for 1--5 min in a Krebs--bicarbonate medium, pH 7.4. In the crude nuclear fraction, 25 mM taurine produced a decrease of 50% in 45Ca2+ uptake; in the crude synaptosomal fraction, taurine reduced 45Ca2+ accumulation by 70%; the maximum inhibitory effect of taurine on 45Ca2+ uptake (80%) was observed in a fraction containing outer segments and pigment epithelium cells. Taurine effect was specific, dose-dependent and related to osmotically sensitive particles. The results suggest a role for taurine in the regulation of calcium fluxes in the retina.", "contents": "Taurine effects on 45Ca2+ transport in retinal subcellular fractions. The effect of taurine on 45Ca2+ transport by subcellular fractions from the chick retina was examined. An inhibitory action of taurine on 45Ca2+ uptake was observed in retinal fractions incubated for 1--5 min in a Krebs--bicarbonate medium, pH 7.4. In the crude nuclear fraction, 25 mM taurine produced a decrease of 50% in 45Ca2+ uptake; in the crude synaptosomal fraction, taurine reduced 45Ca2+ accumulation by 70%; the maximum inhibitory effect of taurine on 45Ca2+ uptake (80%) was observed in a fraction containing outer segments and pigment epithelium cells. Taurine effect was specific, dose-dependent and related to osmotically sensitive particles. The results suggest a role for taurine in the regulation of calcium fluxes in the retina."}
{"id": "PMID:466465", "title": "Distribution of hypertrophied locus coeruleus projection to adult cerebellum after neonatal 6-hydroxydopamine.", "content": "Following treatment as neonates with a high subcutaneous dose of 6-hydroxydopamine (6-OHDA), the projections of the locus coeruleus were mapped in the brains of adult rats. This was done using the technique of unilateral lesions in the nucleus followed by simultaneous determinations of norepinephrine (NE) levels, dopamine-beta-hydroxylase (DBH) activity and synaptosomal [3H]NE uptake in various terminal areas. In particular the cerebellum was subdivided into 3 areas in order to assess any changes from normal in the distribution of the hypertrophied noradrenergic projections here. In vehicle-control rats the lesions resulted in an 80--85% loss of NE in the parietal cortex ipsilateral to the lesion and a 15--20% loss contralaterally. In the control cerebellum the locus coeruleus projection, based upon changes in all 3 markers, is distributed 2/3 ipsilaterally and 1/3 contralaterally with the same pattern present in all 3 subregions. The neonatal 6-OHDA treatment resulted in virtually complete loss of noradrenergic terminals in the cerebral cortex. Following neonatal 6-OHDA treatment cerebellar levels of NE, DBH and [3H]NE uptake increased by between 20--60%, with the smallest increases occurring in [3H]NE uptake. In these rats the locus coeruleus accounted for at least 75--80% of the cerebellar noradrenergic parameters. Unlike control rats however, the lesions in these rats produced only ipsilateral decreases in NE and DBH. On the other hand changes in [3H]NE uptake indicated a normal 2/3 ipsilateral, 1/3 contralateral pattern. It is suggested that two separable events occur in the noradrenergic projection to the cerebellum. The first is the regeneration of an increased number of nerve terminals, or sprouting, and the second is a build-up of synaptic vesicles, or collateral accumulation. The sprouting, judging from the [3H]NE uptake data, occurs with apparently normal distribution, but the accumulation of NE and DBH is confined predominantly to the ipsilaterally projecting axon terminals. This may be the consequence of collateral accumulation resulting from the degeneration of the largely ipsilateral coeruleocortical projection.", "contents": "Distribution of hypertrophied locus coeruleus projection to adult cerebellum after neonatal 6-hydroxydopamine. Following treatment as neonates with a high subcutaneous dose of 6-hydroxydopamine (6-OHDA), the projections of the locus coeruleus were mapped in the brains of adult rats. This was done using the technique of unilateral lesions in the nucleus followed by simultaneous determinations of norepinephrine (NE) levels, dopamine-beta-hydroxylase (DBH) activity and synaptosomal [3H]NE uptake in various terminal areas. In particular the cerebellum was subdivided into 3 areas in order to assess any changes from normal in the distribution of the hypertrophied noradrenergic projections here. In vehicle-control rats the lesions resulted in an 80--85% loss of NE in the parietal cortex ipsilateral to the lesion and a 15--20% loss contralaterally. In the control cerebellum the locus coeruleus projection, based upon changes in all 3 markers, is distributed 2/3 ipsilaterally and 1/3 contralaterally with the same pattern present in all 3 subregions. The neonatal 6-OHDA treatment resulted in virtually complete loss of noradrenergic terminals in the cerebral cortex. Following neonatal 6-OHDA treatment cerebellar levels of NE, DBH and [3H]NE uptake increased by between 20--60%, with the smallest increases occurring in [3H]NE uptake. In these rats the locus coeruleus accounted for at least 75--80% of the cerebellar noradrenergic parameters. Unlike control rats however, the lesions in these rats produced only ipsilateral decreases in NE and DBH. On the other hand changes in [3H]NE uptake indicated a normal 2/3 ipsilateral, 1/3 contralateral pattern. It is suggested that two separable events occur in the noradrenergic projection to the cerebellum. The first is the regeneration of an increased number of nerve terminals, or sprouting, and the second is a build-up of synaptic vesicles, or collateral accumulation. The sprouting, judging from the [3H]NE uptake data, occurs with apparently normal distribution, but the accumulation of NE and DBH is confined predominantly to the ipsilaterally projecting axon terminals. This may be the consequence of collateral accumulation resulting from the degeneration of the largely ipsilateral coeruleocortical projection."}
{"id": "PMID:466466", "title": "Autorhythmicity of spontaneous interictal spike discharge at hippocampal penicillin foci.", "content": "Penicillin-induced epileptogenic foci in the cat hippocampus show a marked tendency for brief but periodic seizure discharges known as 'interictal spikes' (IS). Here, each IS is shown to be followed by a marked elevation and subsequent slow fall-off of the focal seizure threshold. The time constant of this process approximates the spontaneous inter-IS interval and these two parameters appear to vary in concert. The timing of the IS train is always reset by interjected ISs but not by stimuli that are subthreshold for the IS. In sum, this modulation of focal excitability does not appear to be imposed by local or projected rhythmic activity other than that initiated by the IS itself. The firing patterns of the majority of observed hippocampal single units in the vicinity of the focus show a prolonged suppression of spontaneous firing for from 2 to 10 sec or more after each IS, independent of whether the IS was spontaneous or elicited. A smaller number of units show delayed, intense activation following each IS. Both of these forms of response appear to originate from large cells in and near the pyramidal cell body layer. Assuming that these single unit data represent a sampling of pyramidal cell discharge, then the prevalence of a prolonged post-IS pause suggests that the rhythmicity of spontaneous penicillin foci derives from an inhibitory phasing of the population based paroxysmal activity. The periodic spontaneous IS discharge can be viewed, therefore, as a locally regulated, autorhythmic process impressed upon the activity of the neuronal population by the development of a functional suppression of unit activity following each IS.", "contents": "Autorhythmicity of spontaneous interictal spike discharge at hippocampal penicillin foci. Penicillin-induced epileptogenic foci in the cat hippocampus show a marked tendency for brief but periodic seizure discharges known as 'interictal spikes' (IS). Here, each IS is shown to be followed by a marked elevation and subsequent slow fall-off of the focal seizure threshold. The time constant of this process approximates the spontaneous inter-IS interval and these two parameters appear to vary in concert. The timing of the IS train is always reset by interjected ISs but not by stimuli that are subthreshold for the IS. In sum, this modulation of focal excitability does not appear to be imposed by local or projected rhythmic activity other than that initiated by the IS itself. The firing patterns of the majority of observed hippocampal single units in the vicinity of the focus show a prolonged suppression of spontaneous firing for from 2 to 10 sec or more after each IS, independent of whether the IS was spontaneous or elicited. A smaller number of units show delayed, intense activation following each IS. Both of these forms of response appear to originate from large cells in and near the pyramidal cell body layer. Assuming that these single unit data represent a sampling of pyramidal cell discharge, then the prevalence of a prolonged post-IS pause suggests that the rhythmicity of spontaneous penicillin foci derives from an inhibitory phasing of the population based paroxysmal activity. The periodic spontaneous IS discharge can be viewed, therefore, as a locally regulated, autorhythmic process impressed upon the activity of the neuronal population by the development of a functional suppression of unit activity following each IS."}
{"id": "PMID:466467", "title": "Effects of thyroxine on postnatal cell acquisition in the rat brain.", "content": "The effects of treatment with L-thyroxine (3 micrograms by subcutaneous injection daily from birth) on cell acquisition in the rat brain were studied during the first 3 postnatal weeks. In the forebrain, thyroxine has no effect on cell proliferation in the first 6 days, but it causes decreased cell acquisition from 12 to 21 days so that cell number becomes significantly reduced. Estimates of cell proliferation kinetics and of cell death in the lateral ventricular subependymal layer show no apparent abnormality. In the cerebellum, treatment from birth leads to increased cell proliferation during the first week: in comparison with controls, the rate of [3H]thymidine incorporation into DNA, thymidine kinase activity, and the number of cells both in the major germinal site (external granular layer: EGL) and in the whole cerebellum are elevated. This initial effect of thyroxine appears by day 3 and is short-lived, being no longer evident after day 6. The build-up of cell numbers in the EGL at day 6 seems to be related to a preceding, transient retardation of cell migration from this layer rather than to an acceleration of cell replication, since cell cycle parameters are normal. From day 12 the rate of [3H]thymidine incorporation into DNA is severely reduced in treated rats. Advancement of cellular differentiation rather than increased cell death in the EGL appears to be involved in this phenomenon.", "contents": "Effects of thyroxine on postnatal cell acquisition in the rat brain. The effects of treatment with L-thyroxine (3 micrograms by subcutaneous injection daily from birth) on cell acquisition in the rat brain were studied during the first 3 postnatal weeks. In the forebrain, thyroxine has no effect on cell proliferation in the first 6 days, but it causes decreased cell acquisition from 12 to 21 days so that cell number becomes significantly reduced. Estimates of cell proliferation kinetics and of cell death in the lateral ventricular subependymal layer show no apparent abnormality. In the cerebellum, treatment from birth leads to increased cell proliferation during the first week: in comparison with controls, the rate of [3H]thymidine incorporation into DNA, thymidine kinase activity, and the number of cells both in the major germinal site (external granular layer: EGL) and in the whole cerebellum are elevated. This initial effect of thyroxine appears by day 3 and is short-lived, being no longer evident after day 6. The build-up of cell numbers in the EGL at day 6 seems to be related to a preceding, transient retardation of cell migration from this layer rather than to an acceleration of cell replication, since cell cycle parameters are normal. From day 12 the rate of [3H]thymidine incorporation into DNA is severely reduced in treated rats. Advancement of cellular differentiation rather than increased cell death in the EGL appears to be involved in this phenomenon."}
{"id": "PMID:466468", "title": "Facilitation of memory consolidation by vasopressin: mediation by terminals of the dorsal noradrenergic bundle?", "content": "Administration of arginine-vasopressin (AVP, 5 micrograms, s.c.) immediately after the learning trial results in a long-term facilitation of a one-trial learning passive avoidance response. This effect of AVP is absent in animals with prior destruction of the ascending dorsal noradrenergic bundle by bilateral microinjection of 6-hydroxydopamine (6-OHDA). Postlearning local microinjection of a minute amount of AVP via chronically implanted cannulae into the locus coeruleus did not influence passive avoidance behavior. Upon injection into the midbrain dorsal raphe nucleus, however AVP facilitated passive avoidance behavior. This effect, however, was absent in rats receiving previous microinjection of 5,6-dihydroxytryptamine (5,6-DHT) or of 6-OHDA into the dorsal raphe nucleus. Bilateral 6-OHDA-induced lesions of the nucleus accumbens or 5,6-DHT-induced destruction of the dorsal raphe nucleus did not prevent the effect of AVP administered subcutaneously. The data suggest that vasopressin facilitates memory consolidation processes by modulating noradrenergic neurotransmission in terminals of the dorsal noradrenergic bundle. The serotoninergic neuronal network originating from the dorsal raphe nucleus has a secondary--norepinephrine-mediated--influence upon these processes.", "contents": "Facilitation of memory consolidation by vasopressin: mediation by terminals of the dorsal noradrenergic bundle? Administration of arginine-vasopressin (AVP, 5 micrograms, s.c.) immediately after the learning trial results in a long-term facilitation of a one-trial learning passive avoidance response. This effect of AVP is absent in animals with prior destruction of the ascending dorsal noradrenergic bundle by bilateral microinjection of 6-hydroxydopamine (6-OHDA). Postlearning local microinjection of a minute amount of AVP via chronically implanted cannulae into the locus coeruleus did not influence passive avoidance behavior. Upon injection into the midbrain dorsal raphe nucleus, however AVP facilitated passive avoidance behavior. This effect, however, was absent in rats receiving previous microinjection of 5,6-dihydroxytryptamine (5,6-DHT) or of 6-OHDA into the dorsal raphe nucleus. Bilateral 6-OHDA-induced lesions of the nucleus accumbens or 5,6-DHT-induced destruction of the dorsal raphe nucleus did not prevent the effect of AVP administered subcutaneously. The data suggest that vasopressin facilitates memory consolidation processes by modulating noradrenergic neurotransmission in terminals of the dorsal noradrenergic bundle. The serotoninergic neuronal network originating from the dorsal raphe nucleus has a secondary--norepinephrine-mediated--influence upon these processes."}
{"id": "PMID:466469", "title": "Biochemical mapping of the noradrenergic ventral bundle projection sites: evidence for a noradrenergic--dopaminergic interaction.", "content": "Norepinephrine (NE) and dopamine (DA) concentration and dopamine turnover were measured 12 days after a unilateral or bilateral noradrenergic ventral bundle (VB) transection to determine the noradrenergic projection sites and possible interactions with dopaminergic systems. Both bilateral and unilateral VB transection resulted in a significant reduction of NE of the nucleus accumbens, lateral septal nucleus, medial forebrain bundle, ventromedial nucleus, dorsomedial nucleus and medial amygdaloid nucleus. Bilateral transection also decreased NE content of the median eminence and the periventricular and arcuate nuclei. In the medial preoptic nucleus, the nucleus interstitialis striae terminalis and the central gray catecholamine area, bilateral transection significantly decreased NE concentrations while unilateral lesions had no significant effect. The anterior hypothalamic, lateral preoptic, and paraventricular nuclei responded to bilateral VB transection with a decrease in NE concentration and to unilateral lesion with a bilateral increase in NE. In the dorsal hippocampus and the caudate nucleus, bilateral lesions had no effect on NE concentrations while unilateral transection significantly decreased NE concentrations. Regions in which neither bilateral nor unilateral VB transection produced a significant change in NE content are the olfactory tubercle, the nucleus tractus diagonalis, substantia nigra pars compacta and reticulata, ventral tegmental area, habenula, superior colliculus, and the cingulate and piriform cortices. Transection of the noradrenergic ventral bundle also produced changes in dopaminergic systems suggesting a noradrenergic--dopaminergic interaction. Bilateral VB transection decreased the dopamine concentration and turnover in the nucleus accumbens, increased steady-state levels and turnover in the nucleus tractus diagonalis and increased dopamine concentration in the lateral septum. Unilateral VB transection decreased DA concentration bilaterally in the caudate nucleus, olfactory tubercle, nucleus accumbens and the nucleus interstitialis striae terminalis but increased concentrations in the substantia nigra pars reticulata (ipsilateral) and in the ventral tegmental area (bilateral). These results indicate a broad projection field for the noradrenergic ventral bundle and suggest a noradrenergic--dopaminergic interaction.", "contents": "Biochemical mapping of the noradrenergic ventral bundle projection sites: evidence for a noradrenergic--dopaminergic interaction. Norepinephrine (NE) and dopamine (DA) concentration and dopamine turnover were measured 12 days after a unilateral or bilateral noradrenergic ventral bundle (VB) transection to determine the noradrenergic projection sites and possible interactions with dopaminergic systems. Both bilateral and unilateral VB transection resulted in a significant reduction of NE of the nucleus accumbens, lateral septal nucleus, medial forebrain bundle, ventromedial nucleus, dorsomedial nucleus and medial amygdaloid nucleus. Bilateral transection also decreased NE content of the median eminence and the periventricular and arcuate nuclei. In the medial preoptic nucleus, the nucleus interstitialis striae terminalis and the central gray catecholamine area, bilateral transection significantly decreased NE concentrations while unilateral lesions had no significant effect. The anterior hypothalamic, lateral preoptic, and paraventricular nuclei responded to bilateral VB transection with a decrease in NE concentration and to unilateral lesion with a bilateral increase in NE. In the dorsal hippocampus and the caudate nucleus, bilateral lesions had no effect on NE concentrations while unilateral transection significantly decreased NE concentrations. Regions in which neither bilateral nor unilateral VB transection produced a significant change in NE content are the olfactory tubercle, the nucleus tractus diagonalis, substantia nigra pars compacta and reticulata, ventral tegmental area, habenula, superior colliculus, and the cingulate and piriform cortices. Transection of the noradrenergic ventral bundle also produced changes in dopaminergic systems suggesting a noradrenergic--dopaminergic interaction. Bilateral VB transection decreased the dopamine concentration and turnover in the nucleus accumbens, increased steady-state levels and turnover in the nucleus tractus diagonalis and increased dopamine concentration in the lateral septum. Unilateral VB transection decreased DA concentration bilaterally in the caudate nucleus, olfactory tubercle, nucleus accumbens and the nucleus interstitialis striae terminalis but increased concentrations in the substantia nigra pars reticulata (ipsilateral) and in the ventral tegmental area (bilateral). These results indicate a broad projection field for the noradrenergic ventral bundle and suggest a noradrenergic--dopaminergic interaction."}
{"id": "PMID:466470", "title": "Peripheral input pathways projecting to the motor cortex in the cat.", "content": "The possibility that the motor cortex receives peripheral input directly from the thalamus was examined using the evoked potential method and the following results were obtained. Potentials in the motor cortex evoked by stimulation of superficial radial (SR) or group II deep radial (DR) nerve were neither abolished nor delayed by ablation of the sensory cortex. Potentials in the motor cortex evoked by stimulation of group II DR nerve were most severely reduced by interruption of the spinocervcial tract. Potentials evoked by stimulation of SR nerve were more severely reduced in the sensory cortex than in the motor cortex by section of the dorsal funiculus or cooling of the cuneate nucleus. The size of evoked potentials in the motor cortex increased rapidly when stimulus intensity to DR nerve exceeded the threshold to group II fibers. The results suggest that some inputs from the SR and group II DR nerves reach the motor cortex without a relay through the sensory cortex.", "contents": "Peripheral input pathways projecting to the motor cortex in the cat. The possibility that the motor cortex receives peripheral input directly from the thalamus was examined using the evoked potential method and the following results were obtained. Potentials in the motor cortex evoked by stimulation of superficial radial (SR) or group II deep radial (DR) nerve were neither abolished nor delayed by ablation of the sensory cortex. Potentials in the motor cortex evoked by stimulation of group II DR nerve were most severely reduced by interruption of the spinocervcial tract. Potentials evoked by stimulation of SR nerve were more severely reduced in the sensory cortex than in the motor cortex by section of the dorsal funiculus or cooling of the cuneate nucleus. The size of evoked potentials in the motor cortex increased rapidly when stimulus intensity to DR nerve exceeded the threshold to group II fibers. The results suggest that some inputs from the SR and group II DR nerves reach the motor cortex without a relay through the sensory cortex."}
{"id": "PMID:466471", "title": "Thalamic projections to the feline motor cortex studied with horseradish peroxidase.", "content": "Cells in the thalamus projecting to the distal forelimb regions of the motor cortex, area 3a, and the sensory cortex were identified with horseradish peroxidase (HRP). These cortical areas were defined by mapping evoked potentials from superficial and deep radial nerve stimulation. Following injections into the motor cortex, the labelled cells were distributed throughout a large region (greater than 2 mm wide) in the ventral portion of the ventral lateral nucleus (VL). The border area between VL and the ventral posterolateral nucleus (VPL) also contained labelled cells. The region containing labelled cells following injections into area 3a was in the rostral, dorsal VPL, and overlapped with the region containing cells labelled by injections into the motor cortex. Cells labelled by injections into the sensory cortex were near the center of VPL distinctly separated from those labelled after injections into either area 3a or the motor cortex.", "contents": "Thalamic projections to the feline motor cortex studied with horseradish peroxidase. Cells in the thalamus projecting to the distal forelimb regions of the motor cortex, area 3a, and the sensory cortex were identified with horseradish peroxidase (HRP). These cortical areas were defined by mapping evoked potentials from superficial and deep radial nerve stimulation. Following injections into the motor cortex, the labelled cells were distributed throughout a large region (greater than 2 mm wide) in the ventral portion of the ventral lateral nucleus (VL). The border area between VL and the ventral posterolateral nucleus (VPL) also contained labelled cells. The region containing labelled cells following injections into area 3a was in the rostral, dorsal VPL, and overlapped with the region containing cells labelled by injections into the motor cortex. Cells labelled by injections into the sensory cortex were near the center of VPL distinctly separated from those labelled after injections into either area 3a or the motor cortex."}
{"id": "PMID:466472", "title": "Receptive fields of thalamic neurons projecting to the motor cortex in the cat.", "content": "The locations and receptive fields of thalamic neurons projecting to the motor cortex were examined and the following results were obtained. (1) Neurons located at the border area between nucleus ventralis lateralis (VL) and nucleus ventralis posterolateralis (VPL) could be activated antidromically from the motor cortex. (2) These neurons received topographically organized somesthetic inputs arising from skin and deep receptors. (3) The receptive fields of neurons in the small area of the motor cortex where these thalamic neurons projected could be examined in 8 instances. In 6 instances, the cortical neurons and the thalamic projection neurons were activated by exactly the same stimuli in the periphery. (4) Removal of the sensory cortex did not significantly change the characteristics of afferent inputs from the periphery to the motor cortex. (5) It is concluded that the motor cortex receives somesthetic inputs directly from the thalamus. The functional role of these inputs was discussed in relation to the known cortical reflexes.", "contents": "Receptive fields of thalamic neurons projecting to the motor cortex in the cat. The locations and receptive fields of thalamic neurons projecting to the motor cortex were examined and the following results were obtained. (1) Neurons located at the border area between nucleus ventralis lateralis (VL) and nucleus ventralis posterolateralis (VPL) could be activated antidromically from the motor cortex. (2) These neurons received topographically organized somesthetic inputs arising from skin and deep receptors. (3) The receptive fields of neurons in the small area of the motor cortex where these thalamic neurons projected could be examined in 8 instances. In 6 instances, the cortical neurons and the thalamic projection neurons were activated by exactly the same stimuli in the periphery. (4) Removal of the sensory cortex did not significantly change the characteristics of afferent inputs from the periphery to the motor cortex. (5) It is concluded that the motor cortex receives somesthetic inputs directly from the thalamus. The functional role of these inputs was discussed in relation to the known cortical reflexes."}
{"id": "PMID:466473", "title": "Role of the paleostriatum in species-typical display behavior of the lizard (Anolis carolinensis).", "content": "In a comparative study of forebrain mechanisms of species-typical behavior, unilateral lesions were placed in the subpallial telencephalon of male green anolis lizards (Anolis carolinsis), in which there is an almost complete decussation of the optic nerve. Responses to a conspecific male antagonist in a territorial confrontation were then tested when the subject's vision was limited to an eye leading to either the normal or lesioned hemisphere. In lizards with lesions involving the paleostriatum and lateral forebrain bundle, there was an elimination of or marked deficit in the performance of the challenge display while signature displays and measures of general activity were not significantly affected. Lesions predominantly in anterior or posterior dorsal ventricular ridge resulted in no significant deficit in any behavioral measure.", "contents": "Role of the paleostriatum in species-typical display behavior of the lizard (Anolis carolinensis). In a comparative study of forebrain mechanisms of species-typical behavior, unilateral lesions were placed in the subpallial telencephalon of male green anolis lizards (Anolis carolinsis), in which there is an almost complete decussation of the optic nerve. Responses to a conspecific male antagonist in a territorial confrontation were then tested when the subject's vision was limited to an eye leading to either the normal or lesioned hemisphere. In lizards with lesions involving the paleostriatum and lateral forebrain bundle, there was an elimination of or marked deficit in the performance of the challenge display while signature displays and measures of general activity were not significantly affected. Lesions predominantly in anterior or posterior dorsal ventricular ridge resulted in no significant deficit in any behavioral measure."}
{"id": "PMID:466474", "title": "Inhibition of spinal cord interneurons by narcotic microinjection and focal electrical stimulation in the periaqueductal central gray matter.", "content": "Single cell evoked activity was recorded from spinal cord interneurons in rats prepared with microinjection cannulae or stimulating electrodes in the periaqueductal central gray matter (PAG). Morphine microinjections (4-16 microgram) inhibited the response evoked by a noxious stimulus in 55% of the wide dynamic range neurons tested. Microinjections of etorphine (0.25-0.5 microgram) inhibited 82% of the nociceptive neurons tested. Neither drug inhibited neurons which responded only to innocuous mechanical stimulation. The inhibition of wide dynamic range neurons produced by narcotic microinjection was antagonized by naloxone (1 mg/kg, i.p.) in 7 of 11 cases. Control experiments indicated that the effects obtained with microinjections could not be attributed to the drugs' diffusion to the spinal cord. Focal electrical stimulation of the PAG inhibited the responses to noxious stimuli of 60% of wide dynamic range neurons but was without effect on the responses of neurons that were activated only by innocuous stimuli. These experiments directly demonstrate that narcotic analgesics restricted to an intracerebral site of action activate a neural system which preferentially inhibits the responses of spinal cord wide dynamic range neurons to noxious stimuli. The system has a specificity for nociceptive input since non-nociceptive neurons were unaffected. Directly comparable results were produced by electrical stimulation of the PAG, supporting the concept that stimulation and narcotics modulate the transmission of nociceptive information by similar mechanisms.", "contents": "Inhibition of spinal cord interneurons by narcotic microinjection and focal electrical stimulation in the periaqueductal central gray matter. Single cell evoked activity was recorded from spinal cord interneurons in rats prepared with microinjection cannulae or stimulating electrodes in the periaqueductal central gray matter (PAG). Morphine microinjections (4-16 microgram) inhibited the response evoked by a noxious stimulus in 55% of the wide dynamic range neurons tested. Microinjections of etorphine (0.25-0.5 microgram) inhibited 82% of the nociceptive neurons tested. Neither drug inhibited neurons which responded only to innocuous mechanical stimulation. The inhibition of wide dynamic range neurons produced by narcotic microinjection was antagonized by naloxone (1 mg/kg, i.p.) in 7 of 11 cases. Control experiments indicated that the effects obtained with microinjections could not be attributed to the drugs' diffusion to the spinal cord. Focal electrical stimulation of the PAG inhibited the responses to noxious stimuli of 60% of wide dynamic range neurons but was without effect on the responses of neurons that were activated only by innocuous stimuli. These experiments directly demonstrate that narcotic analgesics restricted to an intracerebral site of action activate a neural system which preferentially inhibits the responses of spinal cord wide dynamic range neurons to noxious stimuli. The system has a specificity for nociceptive input since non-nociceptive neurons were unaffected. Directly comparable results were produced by electrical stimulation of the PAG, supporting the concept that stimulation and narcotics modulate the transmission of nociceptive information by similar mechanisms."}
{"id": "PMID:466476", "title": "Sodium dependence of the nerve growth factor--regulated hexose uptake in chick embryo ganglionic cells.", "content": "Embryonic dorsal root ganglionic cells, when incubated in vitro in the absence of nerve growth factor (NGF) undergo a general metabolic degeneration which is preceded by certain changes in permeation properties. Previous studies demonstrated that NGF can rapidly modulate permeation properties which regulate the availability to the cell of an important energy source, glucose. Hexose uptake was determined by measuring the ability of the cells to accumulate [3H]labeled 2-deoxy-D-glucose. The work reported here shows that the NGF-dependent portion (about one-third) of the total specific hexose uptake was also dependent on the presence of Na+, with the apparent uptake constant (Kt) for deoxyglucose varying inversely with an external Na+ concentration of 70-140 mM; Vmax was unaffected in this range. Preincubation of ganglionic cells with 10 mM ouabain for 15-60 min, followed by a pulse with [3H]-deoxyglucose, also resulted in 50-95% reduction of the NGF-sensitive uptake. A similar pretreatment of cells with veratridine gave a 25-50% reduction in uptake. The NGF-controlled hexose uptake was also energy dependent, being diminished 50-95% after a 30-90 min preincubation with 2 mM 2,4-dinitrophenol. Uptake activities for other substrates (alpha-aminoisobutyric acid, uridine) which exhibited NGF regulation were likewise Na+-sensitive. These results indicate that availability of major energy substrates to NGF-dependent dorsal root ganglionic neurons is controlled by sodium gradients across their membranes. It is conceivable that NGF provides for maintenance and development of its target neurons by acting on such sodium gradients and, consequently, regulating the intake of essential nutrients.", "contents": "Sodium dependence of the nerve growth factor--regulated hexose uptake in chick embryo ganglionic cells. Embryonic dorsal root ganglionic cells, when incubated in vitro in the absence of nerve growth factor (NGF) undergo a general metabolic degeneration which is preceded by certain changes in permeation properties. Previous studies demonstrated that NGF can rapidly modulate permeation properties which regulate the availability to the cell of an important energy source, glucose. Hexose uptake was determined by measuring the ability of the cells to accumulate [3H]labeled 2-deoxy-D-glucose. The work reported here shows that the NGF-dependent portion (about one-third) of the total specific hexose uptake was also dependent on the presence of Na+, with the apparent uptake constant (Kt) for deoxyglucose varying inversely with an external Na+ concentration of 70-140 mM; Vmax was unaffected in this range. Preincubation of ganglionic cells with 10 mM ouabain for 15-60 min, followed by a pulse with [3H]-deoxyglucose, also resulted in 50-95% reduction of the NGF-sensitive uptake. A similar pretreatment of cells with veratridine gave a 25-50% reduction in uptake. The NGF-controlled hexose uptake was also energy dependent, being diminished 50-95% after a 30-90 min preincubation with 2 mM 2,4-dinitrophenol. Uptake activities for other substrates (alpha-aminoisobutyric acid, uridine) which exhibited NGF regulation were likewise Na+-sensitive. These results indicate that availability of major energy substrates to NGF-dependent dorsal root ganglionic neurons is controlled by sodium gradients across their membranes. It is conceivable that NGF provides for maintenance and development of its target neurons by acting on such sodium gradients and, consequently, regulating the intake of essential nutrients."}
{"id": "PMID:466485", "title": "Modification of a Kowa RC-2 fundus camera for self-photography without the use of mydriatics.", "content": "Research on retinal circulation during space flight required the development of a simple technique to provide self monitoring of blood vessel changes in the fundus without the use of mydriatics. A Kowa RC-2 fundus camera was modified for self-photography by the use of a bite plate for positioning and cross hairs for focusing the subject's retina relative to the film plane. Dilation of the pupils without the use of mydriatics was accomplished by dark adaption of the subject. Pictures were obtained without pupil constriction by the use of a high speed strobe light. This method also has applications for clinical medicine.", "contents": "Modification of a Kowa RC-2 fundus camera for self-photography without the use of mydriatics. Research on retinal circulation during space flight required the development of a simple technique to provide self monitoring of blood vessel changes in the fundus without the use of mydriatics. A Kowa RC-2 fundus camera was modified for self-photography by the use of a bite plate for positioning and cross hairs for focusing the subject's retina relative to the film plane. Dilation of the pupils without the use of mydriatics was accomplished by dark adaption of the subject. Pictures were obtained without pupil constriction by the use of a high speed strobe light. This method also has applications for clinical medicine."}
{"id": "PMID:466487", "title": "Amplitude and fall-time discrimination of unit spike activity using TTL.", "content": "An inexpensive and reliable window discriminator simply constructed using TTL integrated circuits is described. The device will discriminate between unit spikes on the basis of amplitude alone or amplitude and fall-time, and simultaneous analysis of two separate spikes is possible. All pulse outputs and amplitude and fall-time windows may be displayed allowing the setting up of window criteria to proceed under direct visual control.", "contents": "Amplitude and fall-time discrimination of unit spike activity using TTL. An inexpensive and reliable window discriminator simply constructed using TTL integrated circuits is described. The device will discriminate between unit spikes on the basis of amplitude alone or amplitude and fall-time, and simultaneous analysis of two separate spikes is possible. All pulse outputs and amplitude and fall-time windows may be displayed allowing the setting up of window criteria to proceed under direct visual control."}
{"id": "PMID:466486", "title": "Penile reflexes and copulatory behavior in male rats following lesions in the bed nucleus of the stria terminalis.", "content": "Male rats with large lesions in the bed nucleus of the stria terminalis (BNST) had more intromissions preceding ejaculation (if they ejaculated at all), longer intervals between intromissions, and longer postejaculatory refractory periods than control animals. In tests for penile reflexes, BNST males were similar to control males in every respect. The copulatory deficits in BNST males are probably not mediated by a change in penile reflex potential.", "contents": "Penile reflexes and copulatory behavior in male rats following lesions in the bed nucleus of the stria terminalis. Male rats with large lesions in the bed nucleus of the stria terminalis (BNST) had more intromissions preceding ejaculation (if they ejaculated at all), longer intervals between intromissions, and longer postejaculatory refractory periods than control animals. In tests for penile reflexes, BNST males were similar to control males in every respect. The copulatory deficits in BNST males are probably not mediated by a change in penile reflex potential."}
{"id": "PMID:466488", "title": "Electrically quiet temperature controller.", "content": "A circuit for an electrically quiet temperature controller is described that is suitable for controlling the temperature of a neurophysiological preparation above ambient temperature. By not generating spurious electrical signals it facilitates the recording of low level biological potential changes when temperature control is required. The device, constructed of standard electronic components, uses a thermistor as a sensing element and an ordinary heat lamp or heating strip as a heating element.", "contents": "Electrically quiet temperature controller. A circuit for an electrically quiet temperature controller is described that is suitable for controlling the temperature of a neurophysiological preparation above ambient temperature. By not generating spurious electrical signals it facilitates the recording of low level biological potential changes when temperature control is required. The device, constructed of standard electronic components, uses a thermistor as a sensing element and an ordinary heat lamp or heating strip as a heating element."}
{"id": "PMID:466504", "title": "A note on the distribution of glial cells in the molecular layer of the dentate gyrus.", "content": "The distribution of the perikarya of astrocytes and other glial cells in the molecular layer of the dentate gyrus has been studied in gold chloride-sublimate preparations of rats and of normal and reeler mice, and in plastic embedded material from young adult rats. Contrary to previous reports (Rose et al. [7]), we have found no evidence for a distinct \"line\" or \"band\" of astrocyte cell bodies along the interface between the zones of termination of the entorhinal and hippocampal afferents to the dentate gyrus. Indeed, apart from a conspicuous accumulation of astrocytes immediately beneath the pial surface and hippocampal fissure, the distribution of glial cells across the extent of the molecular layer appears to be more or less random. In view of this it is difficult to ascribe a critical role to the astrocytes in either determining the normal distribution of afferent fibers to the dentate gyrus or in promoting their re-organization following its partial deafferentation.", "contents": "A note on the distribution of glial cells in the molecular layer of the dentate gyrus. The distribution of the perikarya of astrocytes and other glial cells in the molecular layer of the dentate gyrus has been studied in gold chloride-sublimate preparations of rats and of normal and reeler mice, and in plastic embedded material from young adult rats. Contrary to previous reports (Rose et al. [7]), we have found no evidence for a distinct \"line\" or \"band\" of astrocyte cell bodies along the interface between the zones of termination of the entorhinal and hippocampal afferents to the dentate gyrus. Indeed, apart from a conspicuous accumulation of astrocytes immediately beneath the pial surface and hippocampal fissure, the distribution of glial cells across the extent of the molecular layer appears to be more or less random. In view of this it is difficult to ascribe a critical role to the astrocytes in either determining the normal distribution of afferent fibers to the dentate gyrus or in promoting their re-organization following its partial deafferentation."}
{"id": "PMID:466506", "title": "Further characterization of fat diet-induced changes in responsiveness to estrogen and glucose.", "content": "Possible reasons for hyperresponsiveness to the anorexic influence of estrogen of rats fed a high-fat (F) diet were explored. It was found that (1) hyperresponsiveness appeared unrelated to body weight, (2) other estrogenic effects (upon vaginal epithelia and upon mating behavior) were normal in F-fed rats, (3) hyperresponsiveness to estrogen was not a result of alterations in brain dopamine or norepinephrine, (4) F-fed rats were also hyperresponsive to postabsorptive satiating effects of intragastric glucose loads. Thus, an altered glucose metabolism of F-fed rats may be related to an increased responsiveness to the anorexic effects of estrogen.", "contents": "Further characterization of fat diet-induced changes in responsiveness to estrogen and glucose. Possible reasons for hyperresponsiveness to the anorexic influence of estrogen of rats fed a high-fat (F) diet were explored. It was found that (1) hyperresponsiveness appeared unrelated to body weight, (2) other estrogenic effects (upon vaginal epithelia and upon mating behavior) were normal in F-fed rats, (3) hyperresponsiveness to estrogen was not a result of alterations in brain dopamine or norepinephrine, (4) F-fed rats were also hyperresponsive to postabsorptive satiating effects of intragastric glucose loads. Thus, an altered glucose metabolism of F-fed rats may be related to an increased responsiveness to the anorexic effects of estrogen."}
{"id": "PMID:466507", "title": "The effects of stimulation of trigeminal sensory afferents upon caudate units in cats.", "content": "This investigation assessed the influences of trigeminal primary sensory afferents upon caudate neuronal activity in locally anesthetized and chloralose anesthetized cats. Afferents from jaw elevator stretch receptors were stimulated via electrodes in the trigeminal mesencephalic nucleus (Mes 5). Afferents from dental and periodontal receptors were stimulated via electrodes in the inferior dental nerve (IDN). Low intensity electrical stimulation of either locus evoked caudate neuronal responses with Mes 5 being more effective. Higher intensity stimulation of IDN in chloralose anesthetized cats was used to determine if thresholds of trigeminal evoked caudate responses corresponded to thresholds of particular fiber groups in the sensory afferent. In all tested units, neuronal responses were only evoked when stimulation was suprathreshold for both A beta and A delta fibers. These data were discussed in relation to processing of oropharyngeal sensory information within the basal ganglia. Possible implications for bucco-lingual dyskinesias were noted.", "contents": "The effects of stimulation of trigeminal sensory afferents upon caudate units in cats. This investigation assessed the influences of trigeminal primary sensory afferents upon caudate neuronal activity in locally anesthetized and chloralose anesthetized cats. Afferents from jaw elevator stretch receptors were stimulated via electrodes in the trigeminal mesencephalic nucleus (Mes 5). Afferents from dental and periodontal receptors were stimulated via electrodes in the inferior dental nerve (IDN). Low intensity electrical stimulation of either locus evoked caudate neuronal responses with Mes 5 being more effective. Higher intensity stimulation of IDN in chloralose anesthetized cats was used to determine if thresholds of trigeminal evoked caudate responses corresponded to thresholds of particular fiber groups in the sensory afferent. In all tested units, neuronal responses were only evoked when stimulation was suprathreshold for both A beta and A delta fibers. These data were discussed in relation to processing of oropharyngeal sensory information within the basal ganglia. Possible implications for bucco-lingual dyskinesias were noted."}
{"id": "PMID:466508", "title": "Influence of thalamic cooling on sensory responses in association cortex.", "content": "Evoked responses to light flash, click, and paw stimuli were recorded in the four cortical association areas in the acutely prepared cat. Average evoked responses (AEP) for 100 trials were formed before, during, and after localized cooling in the midline thalamus. Cooling of the midline thalamus reduced the magnitude of responses to click and paw stimuli, and increased or did not change the responses to light flash. There was very little similarity in trial-to-trial fluctuations of EP magnitude across cortical areas, and cooling did not reduce the similarity that existed. Waveform similarity was reduced by cooling for responses across the cortex to a single stimulus modality, whereas similarity of responses in a single cortical area to all three stimuli was not changed. The temporal components of the AEP influenced by thalamic cooling were different for different stimuli and cortical locations. It was concluded that the midline thalamo-cortical projection through the centromedian area to association cortices is particularly well-differentiated for multisensory responses in a single cortical region, and that the system should not be thought of as nonspecific but as convergent or multisensory.", "contents": "Influence of thalamic cooling on sensory responses in association cortex. Evoked responses to light flash, click, and paw stimuli were recorded in the four cortical association areas in the acutely prepared cat. Average evoked responses (AEP) for 100 trials were formed before, during, and after localized cooling in the midline thalamus. Cooling of the midline thalamus reduced the magnitude of responses to click and paw stimuli, and increased or did not change the responses to light flash. There was very little similarity in trial-to-trial fluctuations of EP magnitude across cortical areas, and cooling did not reduce the similarity that existed. Waveform similarity was reduced by cooling for responses across the cortex to a single stimulus modality, whereas similarity of responses in a single cortical area to all three stimuli was not changed. The temporal components of the AEP influenced by thalamic cooling were different for different stimuli and cortical locations. It was concluded that the midline thalamo-cortical projection through the centromedian area to association cortices is particularly well-differentiated for multisensory responses in a single cortical region, and that the system should not be thought of as nonspecific but as convergent or multisensory."}
{"id": "PMID:466509", "title": "Plasma and cerebrospinal fluid alpha-MSH levels in the rat after hypophysectomy and stimulation of pituitary alpha-MSH secretion.", "content": "Immunoreactive alpha-MSH was measured in cerebrospinal fluid (CSF) and plasma of rats. While treatment with haloperidol increased alpha-MSH levels in the plasma concentration of alpha-MSH in the CSF showed little change. Hypophysectomy also had little effect on the concentration of alpha-MSH in the CSF despite the fall in plasma alpha-MSH levels. This lack of correlation between alpha-MSH levels in the CSF and plasma suggests that the systemic circulation does not deliver alpha-MSH to the CSF. The apparently normal levels of alpha-MSH in the hypothalamus after hypophysectomy suggests that this tissue is able to synthesize alpha-MSH and it is possible that the hypothalamus is a source of the alpha-MSH in the CSF.", "contents": "Plasma and cerebrospinal fluid alpha-MSH levels in the rat after hypophysectomy and stimulation of pituitary alpha-MSH secretion. Immunoreactive alpha-MSH was measured in cerebrospinal fluid (CSF) and plasma of rats. While treatment with haloperidol increased alpha-MSH levels in the plasma concentration of alpha-MSH in the CSF showed little change. Hypophysectomy also had little effect on the concentration of alpha-MSH in the CSF despite the fall in plasma alpha-MSH levels. This lack of correlation between alpha-MSH levels in the CSF and plasma suggests that the systemic circulation does not deliver alpha-MSH to the CSF. The apparently normal levels of alpha-MSH in the hypothalamus after hypophysectomy suggests that this tissue is able to synthesize alpha-MSH and it is possible that the hypothalamus is a source of the alpha-MSH in the CSF."}
{"id": "PMID:466510", "title": "Inhibitory pathway from the frontal cortex to the hypothalamic ventromedial nucleus in the rat.", "content": "Stimulation of the dorsal frontal cortex (area 10, FCtx) evoked two negative waves with short latencies (N1 and N2) followed by a large, longlasting positive wave (P) in the ventromedial nucleus (VMH) as well as in the more dorsal structures in the hypothalamus and the ventral thalamus. Since the N1 followed high frequency stimulation, it was concluded to be due to antidromic activity. Double or triple pulse stimulation summated the P, and reduced the amplitude of the negative waves of the second and the third responses. VMH neuronal discharges were also decreased during the time course of the P. Therefore, the P was concluded to be composed of the IPSPs. VMH neuronal discharges were frequently superimposed on the N2, indicating the origin of this wave to be EPSPs. Strychnine reduced or blocked the inhibition of the VMH neurons caused by not only FCtx stimulation but also glycine application. The results indicate that the VMH receives both excitatory and inhibitory projections from the FCtx, and the inhibition may be transmitted by glycine.", "contents": "Inhibitory pathway from the frontal cortex to the hypothalamic ventromedial nucleus in the rat. Stimulation of the dorsal frontal cortex (area 10, FCtx) evoked two negative waves with short latencies (N1 and N2) followed by a large, longlasting positive wave (P) in the ventromedial nucleus (VMH) as well as in the more dorsal structures in the hypothalamus and the ventral thalamus. Since the N1 followed high frequency stimulation, it was concluded to be due to antidromic activity. Double or triple pulse stimulation summated the P, and reduced the amplitude of the negative waves of the second and the third responses. VMH neuronal discharges were also decreased during the time course of the P. Therefore, the P was concluded to be composed of the IPSPs. VMH neuronal discharges were frequently superimposed on the N2, indicating the origin of this wave to be EPSPs. Strychnine reduced or blocked the inhibition of the VMH neurons caused by not only FCtx stimulation but also glycine application. The results indicate that the VMH receives both excitatory and inhibitory projections from the FCtx, and the inhibition may be transmitted by glycine."}
{"id": "PMID:466517", "title": "The effects of folate deficiency and oestradiol administration on the plasma free amino acid concentrations of the immature hen.", "content": "1. Free amino acid concentrations were measured in the blood plasma of immature pullets receiving a folate-deficient diet and injected with folate and/or oestradiol, with appropriate controls. 2. Folate deficiency caused a decrease and oestradiol administration an increase in the plasma concentration of most of the amino acids studied. 3. Plasma glycine concentration increased in foliate deficiency and decreased with oestradiol treatment. 4. All the observed effects could not be readily explained in terms of the known metabolic role of folate in amino acid metabolism. 5. The results did not indicate that the role of folate in amino acid metabolism was significant in the inhibition of oestradiol-stimulated oviduct growth.", "contents": "The effects of folate deficiency and oestradiol administration on the plasma free amino acid concentrations of the immature hen. 1. Free amino acid concentrations were measured in the blood plasma of immature pullets receiving a folate-deficient diet and injected with folate and/or oestradiol, with appropriate controls. 2. Folate deficiency caused a decrease and oestradiol administration an increase in the plasma concentration of most of the amino acids studied. 3. Plasma glycine concentration increased in foliate deficiency and decreased with oestradiol treatment. 4. All the observed effects could not be readily explained in terms of the known metabolic role of folate in amino acid metabolism. 5. The results did not indicate that the role of folate in amino acid metabolism was significant in the inhibition of oestradiol-stimulated oviduct growth."}
{"id": "PMID:466512", "title": "Hypothalamic unit responses to alimentary perfusions in the anesthetised rat.", "content": "Single unit discharges in the ventromedial hypothalamic nucleus (VMH) and lateral hypothalamic area (LH) were extracellularly recorded in urethane anesthetised female rats, while various solutions were perfused through the stomach or duodenum via implanted polythene tubes. Perfusates, which were maintained at 38 degrees C, were 0.9% (w/v) NaCl, 2.5% NaCl, 5.25% glucose, 30% glucose, 5.0% casein hydrolysate, and liquid food. Only units which did not respond to somatosensory stimuli were tested. One hundred and twenty six units were recorded for periods of up to 3 hr, occasionally longer, in 57 animals. Of these, 74 were recorded during gastric perfusions and 52 during duodenal perfusions. Distension of the stomach elicited changes in firing rate in 16 LH and 8 VMH units. Both increases and decreases in firing rate in response to gastric distension were observed in both the LH and VMH. There was no evidence that nutrient or osmotic properties of the perfusates exerted any modifying influence on hypothalamic unit discharges. Distention of the duodenum by the perfusions elicited changes in firing rate in 12 LH and 6 VMH units. In this case all LH units decreased firing and all VMH units increased firing during distension of the lumen. In addition 2 VMH units appeared to increase their firing rate in association with glucose perfusions. The results are discussed in terms of the role of gastrointestinal feedback to the hypothalamus in food intake regulation.", "contents": "Hypothalamic unit responses to alimentary perfusions in the anesthetised rat. Single unit discharges in the ventromedial hypothalamic nucleus (VMH) and lateral hypothalamic area (LH) were extracellularly recorded in urethane anesthetised female rats, while various solutions were perfused through the stomach or duodenum via implanted polythene tubes. Perfusates, which were maintained at 38 degrees C, were 0.9% (w/v) NaCl, 2.5% NaCl, 5.25% glucose, 30% glucose, 5.0% casein hydrolysate, and liquid food. Only units which did not respond to somatosensory stimuli were tested. One hundred and twenty six units were recorded for periods of up to 3 hr, occasionally longer, in 57 animals. Of these, 74 were recorded during gastric perfusions and 52 during duodenal perfusions. Distension of the stomach elicited changes in firing rate in 16 LH and 8 VMH units. Both increases and decreases in firing rate in response to gastric distension were observed in both the LH and VMH. There was no evidence that nutrient or osmotic properties of the perfusates exerted any modifying influence on hypothalamic unit discharges. Distention of the duodenum by the perfusions elicited changes in firing rate in 12 LH and 6 VMH units. In this case all LH units decreased firing and all VMH units increased firing during distension of the lumen. In addition 2 VMH units appeared to increase their firing rate in association with glucose perfusions. The results are discussed in terms of the role of gastrointestinal feedback to the hypothalamus in food intake regulation."}
{"id": "PMID:466511", "title": "Role of limbic system in the control of hamster growth.", "content": "Rostral septal lesions accelerate somatic growth in adult hamsters. This study tested the hypothesis that this effect results from damage to fibers of passage by observing the effects of transections of septohippocampal and septohypothalamic connections on growth. We attempted to identify these fibers further by (a) measuring spectrofluorometrically changes in the monoamine concentrations in hippocampus, cerebral cortex, corpus striatum, and diencephalon, (b) staining the degenerating axons after septal lesions and the two cuts, and (c) examining the correspondence between such damage and the acceleration of growth. Both knife cuts accelerated somatic growth and were associated (as well as septal lesions) with significant depletions of serotonin (-27 to -57%) and norepinephrine (-27 to -60%) in the hippocampus, with less consistent depletions of these monoamines in the cerebral cortex, and with no changes in regional dopamine content. All three procedures were associated with degeneration in the hippocampal formation and its fiber systems. Thus, fibers interconnecting hippocampus and brainstem, and passing through septum, exert tonic suppression over somatic growth in adult hamsters.", "contents": "Role of limbic system in the control of hamster growth. Rostral septal lesions accelerate somatic growth in adult hamsters. This study tested the hypothesis that this effect results from damage to fibers of passage by observing the effects of transections of septohippocampal and septohypothalamic connections on growth. We attempted to identify these fibers further by (a) measuring spectrofluorometrically changes in the monoamine concentrations in hippocampus, cerebral cortex, corpus striatum, and diencephalon, (b) staining the degenerating axons after septal lesions and the two cuts, and (c) examining the correspondence between such damage and the acceleration of growth. Both knife cuts accelerated somatic growth and were associated (as well as septal lesions) with significant depletions of serotonin (-27 to -57%) and norepinephrine (-27 to -60%) in the hippocampus, with less consistent depletions of these monoamines in the cerebral cortex, and with no changes in regional dopamine content. All three procedures were associated with degeneration in the hippocampal formation and its fiber systems. Thus, fibers interconnecting hippocampus and brainstem, and passing through septum, exert tonic suppression over somatic growth in adult hamsters."}
{"id": "PMID:466518", "title": "The localisation of beta-N-acetyl-glucosaminidase in the oviduct of the domestic fowl.", "content": "1. Beta-N-acetylglucosaminidase [EC 3.2.1.30] activity in the oviduct of the domestic fowl has been studied using biochemical and histochemical methods. 2. The principal source of the enzyme was the isthmus, where the enzyme was localised within the less dense region of the dual density granules which characterise this portion of the oviduct.", "contents": "The localisation of beta-N-acetyl-glucosaminidase in the oviduct of the domestic fowl. 1. Beta-N-acetylglucosaminidase [EC 3.2.1.30] activity in the oviduct of the domestic fowl has been studied using biochemical and histochemical methods. 2. The principal source of the enzyme was the isthmus, where the enzyme was localised within the less dense region of the dual density granules which characterise this portion of the oviduct."}
{"id": "PMID:466513", "title": "Apparatus for EEG recording and electrical stimulation of discrete brain areas in an unrestrained small animal.", "content": "An apparatus that permits the observation of behavior, recording of EEG and electrical stimulation of the brain can be built from readily available and inexpensive components. This type of apparatus has a variety of uses in biomedical research.", "contents": "Apparatus for EEG recording and electrical stimulation of discrete brain areas in an unrestrained small animal. An apparatus that permits the observation of behavior, recording of EEG and electrical stimulation of the brain can be built from readily available and inexpensive components. This type of apparatus has a variety of uses in biomedical research."}
{"id": "PMID:466519", "title": "Variations in plasma prolactin, thyroid hormones, gonadal steroids and growth hormone in turkeys during the induction of egg laying and moult by different photoperiods.", "content": "1. Concentrations of prolactin, growth hormone, testosterone, progesterone, thyroxine and triiodothyronine were measured in the blood plasma of female turkeys during successive periods of egg laying, a decline in lay, a moult induced by a short photoperiod (6 light: 18 dark) and a resumption of egg laying induced by a long photoperiod (16L:8D). 2. Concentrations of prolactin, growth hormone, testosterone and progesterone were higher in laying birds than in birds which were moulting or not laying. 3. The concentration of testosterone, but not of the other hormones studied, increased significantly during the period of profuse moult. 4. Concentrations of the thyroid hormones did not change with the varying physiological condition of the birds. However, the concentration of thyroxine was depressed by the long photoperiod.", "contents": "Variations in plasma prolactin, thyroid hormones, gonadal steroids and growth hormone in turkeys during the induction of egg laying and moult by different photoperiods. 1. Concentrations of prolactin, growth hormone, testosterone, progesterone, thyroxine and triiodothyronine were measured in the blood plasma of female turkeys during successive periods of egg laying, a decline in lay, a moult induced by a short photoperiod (6 light: 18 dark) and a resumption of egg laying induced by a long photoperiod (16L:8D). 2. Concentrations of prolactin, growth hormone, testosterone and progesterone were higher in laying birds than in birds which were moulting or not laying. 3. The concentration of testosterone, but not of the other hormones studied, increased significantly during the period of profuse moult. 4. Concentrations of the thyroid hormones did not change with the varying physiological condition of the birds. However, the concentration of thyroxine was depressed by the long photoperiod."}
{"id": "PMID:466514", "title": "An improved simple tungsten microelectrode.", "content": "An improvement to tungsten-in-glass microelectrode has been made which requires to special skills. It involves the introduction of a gel solution into the glass micropipette which surrounds and binds the tungsten wire along its full length. A significant damping of microphonic effects is produced and a good fit between tungsten and glass near their tips becomes less critical for success. The electrode was found to be very stable over long recording sessions.", "contents": "An improved simple tungsten microelectrode. An improvement to tungsten-in-glass microelectrode has been made which requires to special skills. It involves the introduction of a gel solution into the glass micropipette which surrounds and binds the tungsten wire along its full length. A significant damping of microphonic effects is produced and a good fit between tungsten and glass near their tips becomes less critical for success. The electrode was found to be very stable over long recording sessions."}
{"id": "PMID:466520", "title": "A study of the growth of the alimentary tract of the young cockerel.", "content": "1. The growth of the alimentary tract of male domestic fowl has been described from measurements of the dry weight of the whole and component parts of the tract from hatching to 10 weeks of age. 2. Quadratic regression equations were derived to express the growth of the birds and the growth of their alimentary tracts. 3. From hatching to 2 weeks there were marked alterations in the proportions which the different parts represented of the size of the whole tract.", "contents": "A study of the growth of the alimentary tract of the young cockerel. 1. The growth of the alimentary tract of male domestic fowl has been described from measurements of the dry weight of the whole and component parts of the tract from hatching to 10 weeks of age. 2. Quadratic regression equations were derived to express the growth of the birds and the growth of their alimentary tracts. 3. From hatching to 2 weeks there were marked alterations in the proportions which the different parts represented of the size of the whole tract."}
{"id": "PMID:466521", "title": "Variations in plasma growth hormone concentrations in laying hens.", "content": "1. Variations in plasma concentrations of growth hormone (GH) and luteinising hormone (LH) were measured by radioimmunoassay in blood samples taken during the ovulatory cycle of the domestic hen. 2. A peak in LH concentrationas observed 4 h prior to ovulation. 3. Plasma GH concentrations were quite variable but a significant increase was observed between 2 h before and 2 h after ovulation and the concentration remained elevated for about 4 h thereafter.", "contents": "Variations in plasma growth hormone concentrations in laying hens. 1. Variations in plasma concentrations of growth hormone (GH) and luteinising hormone (LH) were measured by radioimmunoassay in blood samples taken during the ovulatory cycle of the domestic hen. 2. A peak in LH concentrationas observed 4 h prior to ovulation. 3. Plasma GH concentrations were quite variable but a significant increase was observed between 2 h before and 2 h after ovulation and the concentration remained elevated for about 4 h thereafter."}
{"id": "PMID:466515", "title": "Sodium consumption following lesions surrounding the anteroventral third ventricle.", "content": "Consumption of sodium solution was measured in rats with lesions of the anteroventral third ventricle (AV3V) and maintained on sodium deficient chow. Rats with AV3V lesions consumed less sodium than control animals, and increased their sodium consumption following a subcutaneous injection of Formalin. These data indicate that the AV3V ablation alters the nature of the regulatory control of sodium but does not render animals insensitive to plasma sodium levels.", "contents": "Sodium consumption following lesions surrounding the anteroventral third ventricle. Consumption of sodium solution was measured in rats with lesions of the anteroventral third ventricle (AV3V) and maintained on sodium deficient chow. Rats with AV3V lesions consumed less sodium than control animals, and increased their sodium consumption following a subcutaneous injection of Formalin. These data indicate that the AV3V ablation alters the nature of the regulatory control of sodium but does not render animals insensitive to plasma sodium levels."}
{"id": "PMID:466516", "title": "A technique for the controlled fracture of micropipette electrode tips.", "content": "A method is described for the controlled fracture of electrolyte-filled micropipette electrodes, so that their tip diameter and DC resistance becomes suitable for extracellular recording from individual neurones. The technique is simple and has a low wastage rate. The apparatus used is that employed in conventional microelectrode recording.", "contents": "A technique for the controlled fracture of micropipette electrode tips. A method is described for the controlled fracture of electrolyte-filled micropipette electrodes, so that their tip diameter and DC resistance becomes suitable for extracellular recording from individual neurones. The technique is simple and has a low wastage rate. The apparatus used is that employed in conventional microelectrode recording."}
{"id": "PMID:466522", "title": "Effect of diurnal or nocturnal heat stress on egg formation.", "content": "1. Mature laying hens were subjected to ambient temperatures sufficient to maintain body temperature of 43 degrees C for periods of 6 to 7 h during the day (eight periods) or the night (five periods). This did not reduce total daily food consumption. 2. The effect of heat stress during the day was mostly on egg-shell quality, being adverse and significant. During the night heat stress caused a significant decrease in egg production, its effect during the day was less marked in this respect. 3. Heat stress thus seems mostly to affect the early stages of shell formation, while its effect on egg production seems to depend on the time at which it occurs in relation to ovulation. 4. These results suggest that egg production is directly affected by heat stress; prolonged heat stress probably also acts indirectly, by suppression of food intake.", "contents": "Effect of diurnal or nocturnal heat stress on egg formation. 1. Mature laying hens were subjected to ambient temperatures sufficient to maintain body temperature of 43 degrees C for periods of 6 to 7 h during the day (eight periods) or the night (five periods). This did not reduce total daily food consumption. 2. The effect of heat stress during the day was mostly on egg-shell quality, being adverse and significant. During the night heat stress caused a significant decrease in egg production, its effect during the day was less marked in this respect. 3. Heat stress thus seems mostly to affect the early stages of shell formation, while its effect on egg production seems to depend on the time at which it occurs in relation to ovulation. 4. These results suggest that egg production is directly affected by heat stress; prolonged heat stress probably also acts indirectly, by suppression of food intake."}
{"id": "PMID:466523", "title": "Energy and nitrogen metabolism of chickens infected with either Eimeria acervulina or Eimeria tenella.", "content": "1. The effects of sublethal infections of E. acervulina and E. tenella on the energy and nitrogen metabolism of groups of five broilers aged 16 d were studied for 16 d in respiration chambers. 2. The metabolisable energy content of the diet for chickens infected with E. acervulina was 0.689 of its gross energy content and N retention was 42.5 g/100 g N intake compared with 0.738 and 47.1 g respectively, in uninfected pair-fed controls. Chickens infected with E. tenella were similarly affected. 3. Efficiency of utilisation of ME by chickens infected with E. acervulina was 0.43 during the first 8 d after infection, and 0.52 during the second 8 d compared with an overall efficiency by non-infected chickens fed ad libitum of 0.73. Maintenance energy requirement of infected chickens was higher during the first 8 d after infection than during the second 8-d period. 4. Body composition measurements showed that of the total gain in weight of chickens infected with E. acervulina, only 7.5 g/kg gain was fat and 213 g/kg was protein compared with 45 g and 210 g respectively for non infected chickens fed ad libitum. 5. E. acervulina and E. tenella infections reduced the apparent digestibility of total mineral, calcium and phosphorus.", "contents": "Energy and nitrogen metabolism of chickens infected with either Eimeria acervulina or Eimeria tenella. 1. The effects of sublethal infections of E. acervulina and E. tenella on the energy and nitrogen metabolism of groups of five broilers aged 16 d were studied for 16 d in respiration chambers. 2. The metabolisable energy content of the diet for chickens infected with E. acervulina was 0.689 of its gross energy content and N retention was 42.5 g/100 g N intake compared with 0.738 and 47.1 g respectively, in uninfected pair-fed controls. Chickens infected with E. tenella were similarly affected. 3. Efficiency of utilisation of ME by chickens infected with E. acervulina was 0.43 during the first 8 d after infection, and 0.52 during the second 8 d compared with an overall efficiency by non-infected chickens fed ad libitum of 0.73. Maintenance energy requirement of infected chickens was higher during the first 8 d after infection than during the second 8-d period. 4. Body composition measurements showed that of the total gain in weight of chickens infected with E. acervulina, only 7.5 g/kg gain was fat and 213 g/kg was protein compared with 45 g and 210 g respectively for non infected chickens fed ad libitum. 5. E. acervulina and E. tenella infections reduced the apparent digestibility of total mineral, calcium and phosphorus."}
{"id": "PMID:466524", "title": "Energy and nitrogen metabolism of chickens subjected to infection and reinfection with Eimeria acervulina.", "content": "1. The effects of E. acervulina on energy and nitrogen metabolism were studied in respiration chambers on four groups of four broilers aged 16 d: group C was infected with 1.1 x 10(6) oocysts/bird on day 1 and fed ad libitum, while groups A, B and D were pair-fed to group C to day 16 of the experiment. On day 17, groups A and C were given 5.0 x 10(6) oocysts/bird and fed ad libitum, while group B was pair-fed to group A and group D was pair-fed to group C to the end of experiment on day 32. 2. Dietary ME as a proportion of gross energy (0.68) and N retention (42 g/100 g N intake) were reduced in the infected group C compared with pair-fed controls (0.75 and 49 g, respectively) for the period 1 to 16 d. Subsequent challenge of group C saw no effects on the two variables, but metabolisability (0.65) and N retention (33 g) were significantly reduced in group A when infected for the first time on day 17. 3. The daily heat production and food intake of group A infected for the first time with E. acervulina on day 17, and group B, their pair-fed controls, were reduced during days 20 to 25 but no such reductions were observed in group C, receiving a challenge dose of E. acervulina, and group D, non-infected pair-fed to group C. The efficiency of utilisation of ME for growth of groups C and D was 0.60 over the 17 to 32 d period. 4. Challenge of immune birds did not affect the apparent digestibility of total minerals, calcium and phosphorus.", "contents": "Energy and nitrogen metabolism of chickens subjected to infection and reinfection with Eimeria acervulina. 1. The effects of E. acervulina on energy and nitrogen metabolism were studied in respiration chambers on four groups of four broilers aged 16 d: group C was infected with 1.1 x 10(6) oocysts/bird on day 1 and fed ad libitum, while groups A, B and D were pair-fed to group C to day 16 of the experiment. On day 17, groups A and C were given 5.0 x 10(6) oocysts/bird and fed ad libitum, while group B was pair-fed to group A and group D was pair-fed to group C to the end of experiment on day 32. 2. Dietary ME as a proportion of gross energy (0.68) and N retention (42 g/100 g N intake) were reduced in the infected group C compared with pair-fed controls (0.75 and 49 g, respectively) for the period 1 to 16 d. Subsequent challenge of group C saw no effects on the two variables, but metabolisability (0.65) and N retention (33 g) were significantly reduced in group A when infected for the first time on day 17. 3. The daily heat production and food intake of group A infected for the first time with E. acervulina on day 17, and group B, their pair-fed controls, were reduced during days 20 to 25 but no such reductions were observed in group C, receiving a challenge dose of E. acervulina, and group D, non-infected pair-fed to group C. The efficiency of utilisation of ME for growth of groups C and D was 0.60 over the 17 to 32 d period. 4. Challenge of immune birds did not affect the apparent digestibility of total minerals, calcium and phosphorus."}
{"id": "PMID:466545", "title": "Phanton limb sensation under subarachnoid and epidural analgesia - a comparative clinical study of two hundred cases.", "content": "Two hundred patients scheduled for various surgical procedures, under subarachnoid and epidural anaesthesia were divided in two groups of 100 for each technique. Subarachnoid analgesia was obtained with five per cent lidocaine, while epidural analgesia was accomplished with 1.5 per cent or 2 per cent lidocaine with adrenaline. This study shows that phantom sensation is painless and self limiting and that it lasts only for the duration of motor and proprioceptive blockade. It does not require any special treatment except psychotherapy, supplemented if needed by tranquilizers. Patients should be positioned after motor blockade has been established.", "contents": "Phanton limb sensation under subarachnoid and epidural analgesia - a comparative clinical study of two hundred cases. Two hundred patients scheduled for various surgical procedures, under subarachnoid and epidural anaesthesia were divided in two groups of 100 for each technique. Subarachnoid analgesia was obtained with five per cent lidocaine, while epidural analgesia was accomplished with 1.5 per cent or 2 per cent lidocaine with adrenaline. This study shows that phantom sensation is painless and self limiting and that it lasts only for the duration of motor and proprioceptive blockade. It does not require any special treatment except psychotherapy, supplemented if needed by tranquilizers. Patients should be positioned after motor blockade has been established."}
{"id": "PMID:466546", "title": "In vivo neutron activation analysis for bone calcium (INVAA) in malignant hyperthermia susceptible patients.", "content": "Some non-rigid Malignant Hyperthermia Susceptible (MHS) patients have a greater than normal incidence of fractures. Radiologically their long bones are slightly thinner than normal. Excessive numbers of caries because of thin dental enamel may also be present. The Malignant Hyperthermic (MH) defects thus appears to involve cells of bone appendages as well as of muscle. We have measured the concentration of calcium in bone by neutron activation analysis and have found it to be reduced in both non-rigid and rigid malignant hyperthermia susceptible patients. The reductions were particularly marked for the non-rigid patients. Bone calcium concentrations were lower in males than in females and in those under 16 and over 60 years of age. This is additional evidence is favour of the hypothesis that malignant hyperthermia is a widespread defect affecting tissues and organs throughout the body.", "contents": "In vivo neutron activation analysis for bone calcium (INVAA) in malignant hyperthermia susceptible patients. Some non-rigid Malignant Hyperthermia Susceptible (MHS) patients have a greater than normal incidence of fractures. Radiologically their long bones are slightly thinner than normal. Excessive numbers of caries because of thin dental enamel may also be present. The Malignant Hyperthermic (MH) defects thus appears to involve cells of bone appendages as well as of muscle. We have measured the concentration of calcium in bone by neutron activation analysis and have found it to be reduced in both non-rigid and rigid malignant hyperthermia susceptible patients. The reductions were particularly marked for the non-rigid patients. Bone calcium concentrations were lower in males than in females and in those under 16 and over 60 years of age. This is additional evidence is favour of the hypothesis that malignant hyperthermia is a widespread defect affecting tissues and organs throughout the body."}
{"id": "PMID:466547", "title": "Comparison of droperidol, haloperidol and prochlorperazine as postoperative anti-emetics.", "content": "The results of this study demonstrate that prochlorperazine, haloperidol and droperidol are all effective post-operative anti-emetic compounds when compared to saline but vary in onset of activity and duration of action. Haloperidol has the shortest onset of action, being effective within 30 minutes of intravenous administration. Prochlorperazine has an intermediate onset of action and droperidol is the slowest of the three compounds but the only one to provide significant anti-emesis 4-24 hours following administration. Our data suggest that a combination of haloperidol and droperidol may be more effective as an anti-emetic than any one of the compounds used alone.", "contents": "Comparison of droperidol, haloperidol and prochlorperazine as postoperative anti-emetics. The results of this study demonstrate that prochlorperazine, haloperidol and droperidol are all effective post-operative anti-emetic compounds when compared to saline but vary in onset of activity and duration of action. Haloperidol has the shortest onset of action, being effective within 30 minutes of intravenous administration. Prochlorperazine has an intermediate onset of action and droperidol is the slowest of the three compounds but the only one to provide significant anti-emesis 4-24 hours following administration. Our data suggest that a combination of haloperidol and droperidol may be more effective as an anti-emetic than any one of the compounds used alone."}
{"id": "PMID:466548", "title": "Introduction of central venous pressure catheters through arm veins with a high success rate.", "content": "Studies were carried out to develop a more successful method of inserting central venous catheters through arm veins without using electrocardiographic or fluoroscopic monitoring. It was found that a running infusion attached to a Sorensen catheter gave a success rate of 48 per cent. The highest success rates (98 per cent) occurred when the basilic vein was used, with the patient positioned so that the upper part of the body was raised at 45-90 degrees to the horizontal and using a Bardic 16 gauge catheter with a special insertion technique, which is described. This represents a great improvement over the usual success rate of 70 to 80 per cent.", "contents": "Introduction of central venous pressure catheters through arm veins with a high success rate. Studies were carried out to develop a more successful method of inserting central venous catheters through arm veins without using electrocardiographic or fluoroscopic monitoring. It was found that a running infusion attached to a Sorensen catheter gave a success rate of 48 per cent. The highest success rates (98 per cent) occurred when the basilic vein was used, with the patient positioned so that the upper part of the body was raised at 45-90 degrees to the horizontal and using a Bardic 16 gauge catheter with a special insertion technique, which is described. This represents a great improvement over the usual success rate of 70 to 80 per cent."}
{"id": "PMID:466549", "title": "Prophylactic blood patch does not prevent post lumbar puncture headache.", "content": "Of 86 patients in whom accidental puncture of the dura occurred during insertion of a needle for induction of epidural analgeisa for labour and delivery, 11 received a prophylactic blood patch at the completion of delivery. Of the 75 patients with no prophylactic blood patch, 59 per cent developed post-lumbar-puncture headache, while 54 per cent of those who had a prophylactic blood patch developed headache. The prophylactic blood patch appears to have no value in preventing post-lumbar-puncture headache in this small group of patients.", "contents": "Prophylactic blood patch does not prevent post lumbar puncture headache. Of 86 patients in whom accidental puncture of the dura occurred during insertion of a needle for induction of epidural analgeisa for labour and delivery, 11 received a prophylactic blood patch at the completion of delivery. Of the 75 patients with no prophylactic blood patch, 59 per cent developed post-lumbar-puncture headache, while 54 per cent of those who had a prophylactic blood patch developed headache. The prophylactic blood patch appears to have no value in preventing post-lumbar-puncture headache in this small group of patients."}
{"id": "PMID:466550", "title": "Anaesthesia for tracheal resection. Modified use of the Carden Tube.", "content": "Surgical procedures involving sharing of the airway between the anaesthetist and surgeon, such as bronchoscopy, microlaryngeal surgery and tracheal resection impose special problems on the anaesthetist. The surgeon requires unrestricted surgical access, whereas the anaesthetist must ensure adequate and safe pulmonary ventilation and anaesthesia. This case report describes a two stage technique for management of the airway and ventilation during tracheal resection for stenosis. Initially a modified Carden tube with a ventilating injector was used through a tracheostomy stoma and subsequently an orotracheal tube was substituted. Anaesthesia was maintained entirely by the inravenous route. The surgical access was unrestricted and there was no pulmonary contamination.", "contents": "Anaesthesia for tracheal resection. Modified use of the Carden Tube. Surgical procedures involving sharing of the airway between the anaesthetist and surgeon, such as bronchoscopy, microlaryngeal surgery and tracheal resection impose special problems on the anaesthetist. The surgeon requires unrestricted surgical access, whereas the anaesthetist must ensure adequate and safe pulmonary ventilation and anaesthesia. This case report describes a two stage technique for management of the airway and ventilation during tracheal resection for stenosis. Initially a modified Carden tube with a ventilating injector was used through a tracheostomy stoma and subsequently an orotracheal tube was substituted. Anaesthesia was maintained entirely by the inravenous route. The surgical access was unrestricted and there was no pulmonary contamination."}
{"id": "PMID:466551", "title": "Acute airway obstruction: a complication of aluminum tape wrapping of tracheal tubes in laser surgery.", "content": "A case is reported of acute airway obstruction arising from the use of a tracheal tube wrapped with aluminum tape during laryngeal surgery with a carbon dioxide laser. Problems associated with aluminum tape wrapping of tracheal tubes are discussed.", "contents": "Acute airway obstruction: a complication of aluminum tape wrapping of tracheal tubes in laser surgery. A case is reported of acute airway obstruction arising from the use of a tracheal tube wrapped with aluminum tape during laryngeal surgery with a carbon dioxide laser. Problems associated with aluminum tape wrapping of tracheal tubes are discussed."}
{"id": "PMID:466552", "title": "Laryngocele--an anaesthetic hazard.", "content": "The authors report a case in which a previously unrecognized laryngocele in an eight-year-old child was accidentally intubated and inflated after induction of anaesthesia for tonsillectomy. The nature and diagnosis of laryngocele are discussed.", "contents": "Laryngocele--an anaesthetic hazard. The authors report a case in which a previously unrecognized laryngocele in an eight-year-old child was accidentally intubated and inflated after induction of anaesthesia for tonsillectomy. The nature and diagnosis of laryngocele are discussed."}
{"id": "PMID:466553", "title": "Knotting of an epidural catheter: a case report.", "content": "Difficulty with removal of an epidural catheter is described due to formation of a knot near the end of the catheter. It is stressed that in the lumbar area an epidural catheter should never be inserted more than 5 cm into the epidural space, as curling with knot formation may occur. Removal may be attempted by pulling on the catheter if testing of a similar catheter indicates that it will withstand the tension. The patient and the spouse should be kept informed and involved in the decision-making for medico-legal reasons.", "contents": "Knotting of an epidural catheter: a case report. Difficulty with removal of an epidural catheter is described due to formation of a knot near the end of the catheter. It is stressed that in the lumbar area an epidural catheter should never be inserted more than 5 cm into the epidural space, as curling with knot formation may occur. Removal may be attempted by pulling on the catheter if testing of a similar catheter indicates that it will withstand the tension. The patient and the spouse should be kept informed and involved in the decision-making for medico-legal reasons."}
{"id": "PMID:466557", "title": "Relation of precurarization to suxamethonium to provide ease of intubation and to prevent post-suxamethonium muscle pains.", "content": "Tubocurarine 0.05 and 0.07 mg.kg-1, gallamine 0.1 and 0.2 mg.kg-1 and pancuronium 0.01 and 0.02 mg.kg-1 given three minutes before suxamethonium 1.0, 1.5 and 2 mg.kg-1 in groups of 10 patients each (total 210 patients) to compare ease of tracheal intubation and incidence of post-suxamethonium muscle pain. These were compared with a control group of suxamethonium 1.0, 1.5 and 2 mg.kg-1 given alone after thiopentone 5 mg.kg-1. On analysis, tubocurarine 0.07 mg.kg-1 and suxamethonium 2 mg.kg-1 was the ideal combination with the best intubation conditions and the lowest incidence of post-suxamethonium muscle pains. The second best combination was gallamine 0.2 mg.kg-1 and suxamethonium 2 mg.kg-1. Pancuronium 0.01 mg.kg-1 and 0.02 mg.kg-1 in combination with suxamethonium 2 mg.kg-1 were satisfactory, although less efficient than the combination with either tubocurarine or gallamine.", "contents": "Relation of precurarization to suxamethonium to provide ease of intubation and to prevent post-suxamethonium muscle pains. Tubocurarine 0.05 and 0.07 mg.kg-1, gallamine 0.1 and 0.2 mg.kg-1 and pancuronium 0.01 and 0.02 mg.kg-1 given three minutes before suxamethonium 1.0, 1.5 and 2 mg.kg-1 in groups of 10 patients each (total 210 patients) to compare ease of tracheal intubation and incidence of post-suxamethonium muscle pain. These were compared with a control group of suxamethonium 1.0, 1.5 and 2 mg.kg-1 given alone after thiopentone 5 mg.kg-1. On analysis, tubocurarine 0.07 mg.kg-1 and suxamethonium 2 mg.kg-1 was the ideal combination with the best intubation conditions and the lowest incidence of post-suxamethonium muscle pains. The second best combination was gallamine 0.2 mg.kg-1 and suxamethonium 2 mg.kg-1. Pancuronium 0.01 mg.kg-1 and 0.02 mg.kg-1 in combination with suxamethonium 2 mg.kg-1 were satisfactory, although less efficient than the combination with either tubocurarine or gallamine."}
{"id": "PMID:466558", "title": "Abnormal enzyme phenotype (E1a E1f): normal response to succinylcholine.", "content": "The enzyme serum cholinesterase responsible for the hydrolysis of the muscle relaxant succinylcholine exists in the form of several variants. These may be identified in serum by using substances which inhibit their activity to different degrees. The heterozygote for the atypical and fluoride resistant enzymes (E1a E1f) is one of the phenotypes which has been reported to be sinsitive to succinylcholine. A case is described where succinylcholine given on two separate occasions did not induce apnoea in an individual phenotyped as E1a E1f by at least five methods of inhibition. This is the first reported example of such insensitivity to the drug in this phenotype. Temperature activities for the patient's serum over the range of 20 degrees C to 45 degrees C differed from that of an established E1a E1f phenotype used as a control. There was a progressive inactivation of the control serum at temperatures higher than 35 degrees C, as previously reported for this phenotype. Activity in the serum of the subject of this study did not exhibit the peak activity at 35 degrees C but continued to rise and probably reached a peak between 40 degrees C and 45 degrees C. The significance of these results in the context of current methods of phenotyping is discussed.", "contents": "Abnormal enzyme phenotype (E1a E1f): normal response to succinylcholine. The enzyme serum cholinesterase responsible for the hydrolysis of the muscle relaxant succinylcholine exists in the form of several variants. These may be identified in serum by using substances which inhibit their activity to different degrees. The heterozygote for the atypical and fluoride resistant enzymes (E1a E1f) is one of the phenotypes which has been reported to be sinsitive to succinylcholine. A case is described where succinylcholine given on two separate occasions did not induce apnoea in an individual phenotyped as E1a E1f by at least five methods of inhibition. This is the first reported example of such insensitivity to the drug in this phenotype. Temperature activities for the patient's serum over the range of 20 degrees C to 45 degrees C differed from that of an established E1a E1f phenotype used as a control. There was a progressive inactivation of the control serum at temperatures higher than 35 degrees C, as previously reported for this phenotype. Activity in the serum of the subject of this study did not exhibit the peak activity at 35 degrees C but continued to rise and probably reached a peak between 40 degrees C and 45 degrees C. The significance of these results in the context of current methods of phenotyping is discussed."}
{"id": "PMID:466559", "title": "Inline blood gas analysis by gas chromatography in patients during and after coronary artery surgery.", "content": "A system was evaluated of measuring Pao2 and Pao2 by an inline sensor in the brachial artery and gas chromatography. Eight patients having coronary artery vein grafts were studied during anaesthesia, operation, perfusion and for 24 hours afterward. Compared to conventional blood gas analysis by polarography (electrodes), the chromatographic method gave readings for Pao2 which were not significantly different during normothermia. During hypothermic perfusion, the chromatographic system read significantly higher than the bench electrode, due at least in part to a difference in temperature correction. For Pao2 the correlation between the two methods was close and differences were clinically insignificant. The Sentorr blood-gas analyser provides a sensitive, accurate indicator of changes in oxygenation, ventilation and circulation during anaesthesia and in the intensive care unit.", "contents": "Inline blood gas analysis by gas chromatography in patients during and after coronary artery surgery. A system was evaluated of measuring Pao2 and Pao2 by an inline sensor in the brachial artery and gas chromatography. Eight patients having coronary artery vein grafts were studied during anaesthesia, operation, perfusion and for 24 hours afterward. Compared to conventional blood gas analysis by polarography (electrodes), the chromatographic method gave readings for Pao2 which were not significantly different during normothermia. During hypothermic perfusion, the chromatographic system read significantly higher than the bench electrode, due at least in part to a difference in temperature correction. For Pao2 the correlation between the two methods was close and differences were clinically insignificant. The Sentorr blood-gas analyser provides a sensitive, accurate indicator of changes in oxygenation, ventilation and circulation during anaesthesia and in the intensive care unit."}
{"id": "PMID:466560", "title": "Halothane solubility in blood during cardiopulmonary bypass: the effect of haemodilution and hypothermia.", "content": "The influence of haemodilution and cooling on the solubility of halothane in blood during cardiopulmonary bypass was studied in six normothermic and six hypothermic patients undergoing open-heart operations. The solubility of halothane in blood was 2.17 at 30 degrees C and 1.34 at 37 degrees C. There was also an increase in the blood/gas partition coefficient of about 8.7 per cent for each degree celsius of temperature fall. These changes in haemodiluted blood explain the different anaesthetic requirements observed in patients undergoing cardiopulmonary bypass, either at normal temperature or at moderate hypothermia.", "contents": "Halothane solubility in blood during cardiopulmonary bypass: the effect of haemodilution and hypothermia. The influence of haemodilution and cooling on the solubility of halothane in blood during cardiopulmonary bypass was studied in six normothermic and six hypothermic patients undergoing open-heart operations. The solubility of halothane in blood was 2.17 at 30 degrees C and 1.34 at 37 degrees C. There was also an increase in the blood/gas partition coefficient of about 8.7 per cent for each degree celsius of temperature fall. These changes in haemodiluted blood explain the different anaesthetic requirements observed in patients undergoing cardiopulmonary bypass, either at normal temperature or at moderate hypothermia."}
{"id": "PMID:466561", "title": "Intubation and other experiences in cardiac surgery: the consumer's views.", "content": "Recently publications have advocated earlier weaning and early extubation of the trachea in patients after cardiac surgery. Greater comfort of the patients is one of the advantages claimed for this policy. One hundred consecutive adult patients were questioned on the fifth postoperative day to assess the relative comfort or discomfort of the patients during tracheal intubation and ventilation. Overnight tracheal intubation and ventilation were easily tolerated by over 90 per cent of patients who had received morphine and diazepam. There was a high incidence of amnesia. Some of the other claimed advantages appear inconclusive and further study is desirable to elucidate the effect of early removal of the tracheal tube upon the indicence of pulmonary complications.", "contents": "Intubation and other experiences in cardiac surgery: the consumer's views. Recently publications have advocated earlier weaning and early extubation of the trachea in patients after cardiac surgery. Greater comfort of the patients is one of the advantages claimed for this policy. One hundred consecutive adult patients were questioned on the fifth postoperative day to assess the relative comfort or discomfort of the patients during tracheal intubation and ventilation. Overnight tracheal intubation and ventilation were easily tolerated by over 90 per cent of patients who had received morphine and diazepam. There was a high incidence of amnesia. Some of the other claimed advantages appear inconclusive and further study is desirable to elucidate the effect of early removal of the tracheal tube upon the indicence of pulmonary complications."}
{"id": "PMID:466562", "title": "Cardiac effects of magnesium with special reference to anaesthesia: a review.", "content": "The role of magnesium in myocardial metabolism and the effects of magnesium deficiency on myocardial contraction and the electrocardiogram have been described. The causes of a magnesium deficiency have been discussed in some detail. Finally, the inter-relationship between magnesium and the mitochondrial enzyme systems concerned with oxidative phosphorylation is stressed. The early detection of magnesium deficiency by routine methods is imperative for the prevention of abnormal cardiac metabolism and the maintenance of the functional and structural integrity of cardiac muscle during anaesthesia.", "contents": "Cardiac effects of magnesium with special reference to anaesthesia: a review. The role of magnesium in myocardial metabolism and the effects of magnesium deficiency on myocardial contraction and the electrocardiogram have been described. The causes of a magnesium deficiency have been discussed in some detail. Finally, the inter-relationship between magnesium and the mitochondrial enzyme systems concerned with oxidative phosphorylation is stressed. The early detection of magnesium deficiency by routine methods is imperative for the prevention of abnormal cardiac metabolism and the maintenance of the functional and structural integrity of cardiac muscle during anaesthesia."}
{"id": "PMID:466563", "title": "Effect of doxapram on control of breathing in cats.", "content": "The effects of doxapram infusion (0.25 mg.kg-1. min-1) were studied in cats anaesthetized with pentobarbitone (35 mg . kg-1 intraperitoneally). Cats were studied breathing 50 per cent oxygen and the responses to two concentrations of inspired carbon dioxide were measured. Doxapram infusion increased pulmonary ventilation by increasing both tidal volume and respiratory frequency, and also caused increases in the volume inspired in the first 0.5 second after the onset of an inspiration (V0.5) and the pressure generated in the airway 0.5 second after the onset of an inspiration when the airway had been occluded (P degrees 0.5). V 0.5, P degrees 0.5 and the mean inspiratory flow rate (VT/VI) were essentially equivalent indices of inspiratory drive. Doxapram infusion did not alter the effective impedance of the respiratory system (P degrees 0.5/V 0.5). Doxapram infusion increased the ventilatory response to carbon dioxide. The slope of the ventilatory response to carbon dioxide was increased and the response line was shifted to the left. We conclude that the increase in pulmonary ventilation caused by doxapram infusion is due almost entirely to increased inspiratory neuromuscular drive (P degrees 0.5).", "contents": "Effect of doxapram on control of breathing in cats. The effects of doxapram infusion (0.25 mg.kg-1. min-1) were studied in cats anaesthetized with pentobarbitone (35 mg . kg-1 intraperitoneally). Cats were studied breathing 50 per cent oxygen and the responses to two concentrations of inspired carbon dioxide were measured. Doxapram infusion increased pulmonary ventilation by increasing both tidal volume and respiratory frequency, and also caused increases in the volume inspired in the first 0.5 second after the onset of an inspiration (V0.5) and the pressure generated in the airway 0.5 second after the onset of an inspiration when the airway had been occluded (P degrees 0.5). V 0.5, P degrees 0.5 and the mean inspiratory flow rate (VT/VI) were essentially equivalent indices of inspiratory drive. Doxapram infusion did not alter the effective impedance of the respiratory system (P degrees 0.5/V 0.5). Doxapram infusion increased the ventilatory response to carbon dioxide. The slope of the ventilatory response to carbon dioxide was increased and the response line was shifted to the left. We conclude that the increase in pulmonary ventilation caused by doxapram infusion is due almost entirely to increased inspiratory neuromuscular drive (P degrees 0.5)."}
{"id": "PMID:466564", "title": "Efficacy of helium--oxygen mixtures in the management of severe viral and post-intubation croup.", "content": "An appreciable number of children with viral or post-intubation croup progress to respiratory failure necessitating an artificial airway. We report seven such patients with critical airway narrowing in whom we reduced the work of breathing by developing helium rather than air as a carrier gas for oxygen. Assessment of patients by a croup-scoring system and blood gas analysis suggests helium-oxygen mixtures to be a useful alternative to intervention with tracheostomy or tracheal intubation. The rationale and limitations of this treatment are discussed.", "contents": "Efficacy of helium--oxygen mixtures in the management of severe viral and post-intubation croup. An appreciable number of children with viral or post-intubation croup progress to respiratory failure necessitating an artificial airway. We report seven such patients with critical airway narrowing in whom we reduced the work of breathing by developing helium rather than air as a carrier gas for oxygen. Assessment of patients by a croup-scoring system and blood gas analysis suggests helium-oxygen mixtures to be a useful alternative to intervention with tracheostomy or tracheal intubation. The rationale and limitations of this treatment are discussed."}
{"id": "PMID:466565", "title": "Rectal methohexitone for induction of anaesthesia in children.", "content": "Rectal induction of anaesthesia using ten per cent methohexitone is a safe and effective method for young children. It is particularly applicable to the child three months to four years of age. Twenty-five mg.kg-1 of methohexitone should be given and the child should be observed continuously by the anaesthetist until he falls asleep. Equipment to establish an artificial airway and to ventilate the lungs should be immediately at hand. The use of rectal methohexitone does not significantly delay immediate or late recovery after short (30-minute) surgical procedures.", "contents": "Rectal methohexitone for induction of anaesthesia in children. Rectal induction of anaesthesia using ten per cent methohexitone is a safe and effective method for young children. It is particularly applicable to the child three months to four years of age. Twenty-five mg.kg-1 of methohexitone should be given and the child should be observed continuously by the anaesthetist until he falls asleep. Equipment to establish an artificial airway and to ventilate the lungs should be immediately at hand. The use of rectal methohexitone does not significantly delay immediate or late recovery after short (30-minute) surgical procedures."}
{"id": "PMID:466566", "title": "Chronic pain syndromes and their treatment. II. Trigger points.", "content": "Trigger points are distinct areas of focal hyperirritability which give rise to areas of refered pain in well-defined areas of the musculo-skeletal system, sometimes remote from the point itself and not related to it by anatomically definable pathways. While the vast majority of pain manifestations from trigger points are related to the musculo-skeletal system, this need not be invariably so, as has been demonstrated in two of the cases cited, where injection of trigger points in the neck relieved chronic tinnitus. In all manifestations of chronic pain it is recommended that a diligent search be made for such trigger points.", "contents": "Chronic pain syndromes and their treatment. II. Trigger points. Trigger points are distinct areas of focal hyperirritability which give rise to areas of refered pain in well-defined areas of the musculo-skeletal system, sometimes remote from the point itself and not related to it by anatomically definable pathways. While the vast majority of pain manifestations from trigger points are related to the musculo-skeletal system, this need not be invariably so, as has been demonstrated in two of the cases cited, where injection of trigger points in the neck relieved chronic tinnitus. In all manifestations of chronic pain it is recommended that a diligent search be made for such trigger points."}
{"id": "PMID:466567", "title": "Foetal monitoring during general surgery: a case report.", "content": "A case is reported in which the foetal heart was monitored by a Doppler apparatus during and after an operation on the kidney in a patient who was 27 weeks pregnant. This monitoring proved useful in detecting alterations of foetal heart rate which occurred during positioning of the patient.", "contents": "Foetal monitoring during general surgery: a case report. A case is reported in which the foetal heart was monitored by a Doppler apparatus during and after an operation on the kidney in a patient who was 27 weeks pregnant. This monitoring proved useful in detecting alterations of foetal heart rate which occurred during positioning of the patient."}
{"id": "PMID:466568", "title": "Anaesthesia for facial rhytidectomy.", "content": "Observations on anaesthesia for facial rhytidectomy in 200 patients, without tracheal intubation or artificial ventilation, are reported in this survey.", "contents": "Anaesthesia for facial rhytidectomy. Observations on anaesthesia for facial rhytidectomy in 200 patients, without tracheal intubation or artificial ventilation, are reported in this survey."}
{"id": "PMID:466569", "title": "A \"last ditch\" airway.", "content": "In a patient whose airway is in jeopardy after unsuccessful attempts at conservative measures to clear it and after failed attempts at intubation, the intravenous tubing connector spike may be considered as an emergency percutaneous tracheostomy device. It is readily available wherever physicians have intravenous solutions at hand. It is inserted through the cricothyroid space with the help of a scalpel blade or other sharp instrument such as a pair of scissors. A severed spike can be as easily carried in a pocket, purse or medical bag as can an intravenous cannula. A number 11 scalpel blade fits neatly between two credit cards. The shape of the spike makes it fortuitously adaptable to intermittent positive pressure ventilation by mouth or standard resuscitation equipment. If the patient is otherwise well enough, it is of sufficient bore to allow spontaneous respiration. I have been fortunate not to have had the opportunity to use this device in an emergency situation as of the time of the submission of this paper.", "contents": "A \"last ditch\" airway. In a patient whose airway is in jeopardy after unsuccessful attempts at conservative measures to clear it and after failed attempts at intubation, the intravenous tubing connector spike may be considered as an emergency percutaneous tracheostomy device. It is readily available wherever physicians have intravenous solutions at hand. It is inserted through the cricothyroid space with the help of a scalpel blade or other sharp instrument such as a pair of scissors. A severed spike can be as easily carried in a pocket, purse or medical bag as can an intravenous cannula. A number 11 scalpel blade fits neatly between two credit cards. The shape of the spike makes it fortuitously adaptable to intermittent positive pressure ventilation by mouth or standard resuscitation equipment. If the patient is otherwise well enough, it is of sufficient bore to allow spontaneous respiration. I have been fortunate not to have had the opportunity to use this device in an emergency situation as of the time of the submission of this paper."}
{"id": "PMID:466574", "title": "Effects of apomorphine on thermoregulatory responses of rats to different ambient temperatures.", "content": "Either systemic or central administration of apomorphine produced dose-related decreases in rectal temperature at ambient temperatures (Ta) of 8 and 22 degrees C in rats. At Ta = 8 degrees C, the hypothermia was brought about by a decrease in metabolic rate (M). At Ta = 22 degrees C, the hypothermia was due to an increase in mean skin temperature, an increase in respiratory evaporative heat loss (Eres) and a decrease in M. This increased mean skin temperature was due to increased tail and foot skin temperatures. However, at Ta = 29 degrees C, apomorphine produced increased rectal temperatures due to increased M and decreased Eres. Moreover, the apomorphine-induced hypothermia or hyperthermia was antagonized by either haloperidol or 6-hydroxydopamine, but not by 5,6-dihydroxytryptamine. The data indicate that apomorphine acts on dopamine neurons within brain, with both pre- and post-synaptic sites of action, to influence body temperature.", "contents": "Effects of apomorphine on thermoregulatory responses of rats to different ambient temperatures. Either systemic or central administration of apomorphine produced dose-related decreases in rectal temperature at ambient temperatures (Ta) of 8 and 22 degrees C in rats. At Ta = 8 degrees C, the hypothermia was brought about by a decrease in metabolic rate (M). At Ta = 22 degrees C, the hypothermia was due to an increase in mean skin temperature, an increase in respiratory evaporative heat loss (Eres) and a decrease in M. This increased mean skin temperature was due to increased tail and foot skin temperatures. However, at Ta = 29 degrees C, apomorphine produced increased rectal temperatures due to increased M and decreased Eres. Moreover, the apomorphine-induced hypothermia or hyperthermia was antagonized by either haloperidol or 6-hydroxydopamine, but not by 5,6-dihydroxytryptamine. The data indicate that apomorphine acts on dopamine neurons within brain, with both pre- and post-synaptic sites of action, to influence body temperature."}
{"id": "PMID:466575", "title": "Effect of acute exercise on tissue free fatty acids in untrained rats.", "content": "The effects of a single bout of swimming on free fatty acids (FFA) in adipose tissue, heart, skeletal muscle, and serum were examined. Surprisingly, in previously untrained rats, FFA were elevated (P less than 0.001) in epididymal, inguinal, and retroperitoneal adipose depots 48 h after a 2-h swim. FFA in the three fat depots returned to resting levels 96 h after exercise. In heart, soleus, and fast-red fibers of the quadriceps, FFA remained elevated (P less than 0.01) for as long as 72 h after the 2-h swim. Serum FFA were still elevated (P less than 0.001) 96 h after swimming but not after 168 h. These results provide evidence that the rise in FFA is an acute effect of exercise and not a cellular adaptation resulting from daily episodes of lipolysis induced by exercise training. In a separate experiment, involving the adaptive response to endurance exercise, adipocytes from epididymal, inguinal, and retroperitoneal depots were reduced in size (P less than 0.001) to approximately the same degree. These results provide evidence that adipocytes from each depot contribute equally in meeting the energy needs of muscle during repeated bouts of endurance exercise.", "contents": "Effect of acute exercise on tissue free fatty acids in untrained rats. The effects of a single bout of swimming on free fatty acids (FFA) in adipose tissue, heart, skeletal muscle, and serum were examined. Surprisingly, in previously untrained rats, FFA were elevated (P less than 0.001) in epididymal, inguinal, and retroperitoneal adipose depots 48 h after a 2-h swim. FFA in the three fat depots returned to resting levels 96 h after exercise. In heart, soleus, and fast-red fibers of the quadriceps, FFA remained elevated (P less than 0.01) for as long as 72 h after the 2-h swim. Serum FFA were still elevated (P less than 0.001) 96 h after swimming but not after 168 h. These results provide evidence that the rise in FFA is an acute effect of exercise and not a cellular adaptation resulting from daily episodes of lipolysis induced by exercise training. In a separate experiment, involving the adaptive response to endurance exercise, adipocytes from epididymal, inguinal, and retroperitoneal depots were reduced in size (P less than 0.001) to approximately the same degree. These results provide evidence that adipocytes from each depot contribute equally in meeting the energy needs of muscle during repeated bouts of endurance exercise."}
{"id": "PMID:466576", "title": "Electrical coupling in rat myometrium during pregnancy.", "content": "Gap junctions were found in rat myometrium only during parturition of abortion. A comparison was made between the values of the length constants obtained from longitudinally cut strips of rat myometrium at the midterm stage of pregnancy and during parturition. No significant difference was found between these values. No significant difference was found between length constants measured in myometrium from rats near term (22 days) and those during parturition, or between midterm myometrium and myometrium taken from animals aborting at midterm following ovariectomy. It was concluded that the appearance of gap junctions in parturient myometrium does not alter the spread of passive current in the longitudinal axis of the cells in these tissues. It is suggested that gap junctions are not required for spread of passive current, and that other structures may provide an alternate path.", "contents": "Electrical coupling in rat myometrium during pregnancy. Gap junctions were found in rat myometrium only during parturition of abortion. A comparison was made between the values of the length constants obtained from longitudinally cut strips of rat myometrium at the midterm stage of pregnancy and during parturition. No significant difference was found between these values. No significant difference was found between length constants measured in myometrium from rats near term (22 days) and those during parturition, or between midterm myometrium and myometrium taken from animals aborting at midterm following ovariectomy. It was concluded that the appearance of gap junctions in parturient myometrium does not alter the spread of passive current in the longitudinal axis of the cells in these tissues. It is suggested that gap junctions are not required for spread of passive current, and that other structures may provide an alternate path."}
{"id": "PMID:466577", "title": "Effect of allyl isothiocyanate on plasma and urinary concentrations of some biochemical entities in the rat.", "content": "Allyl isothiocyanate (AITC) is a constituent of several plants of the family Cruciferae that are commonly used as food. This study investigated the effect of feeding AITC to male Sprague-Dawley rats on their plasma glucose and uric acid levels as well as on the urinary concentrations of glucose, 17-ketosteroids (17-KS), creatinine, and uric acid. Other test compounds included were thyroxine (T4) and thiouracil (TU). AITC caused a highly significant (P smaller than or equal to 0.01) depression in the plasma glucose and uric acid levels compared with the control. TU caused a significant depression only of the plasma glucose. T4, on the other hand, significantly increased the levels of both glucose and uric acid. The AITC-treated rats voided twice as much urine as the controls or those receiving TU or injected with T4. The 24-h excretion of glucose, uric acid, and creatinine was significantly (P smaller than or equal to 0.01) higher in animals fed AITC than in those consuming the control diet, while the excretion of 17-KS was significantly lower. Results on an equal urine volume basis showed that differences in the excretion of glucose and creatinine were related to differences in the urine volume. TU significantly depressed excretion of all the compounds but glucose. The effect of T4 on the excretion of 17-KS and uric acid resembled that of AITC and TU, thus showing that these compounds depressed the androgenic function of the animal.", "contents": "Effect of allyl isothiocyanate on plasma and urinary concentrations of some biochemical entities in the rat. Allyl isothiocyanate (AITC) is a constituent of several plants of the family Cruciferae that are commonly used as food. This study investigated the effect of feeding AITC to male Sprague-Dawley rats on their plasma glucose and uric acid levels as well as on the urinary concentrations of glucose, 17-ketosteroids (17-KS), creatinine, and uric acid. Other test compounds included were thyroxine (T4) and thiouracil (TU). AITC caused a highly significant (P smaller than or equal to 0.01) depression in the plasma glucose and uric acid levels compared with the control. TU caused a significant depression only of the plasma glucose. T4, on the other hand, significantly increased the levels of both glucose and uric acid. The AITC-treated rats voided twice as much urine as the controls or those receiving TU or injected with T4. The 24-h excretion of glucose, uric acid, and creatinine was significantly (P smaller than or equal to 0.01) higher in animals fed AITC than in those consuming the control diet, while the excretion of 17-KS was significantly lower. Results on an equal urine volume basis showed that differences in the excretion of glucose and creatinine were related to differences in the urine volume. TU significantly depressed excretion of all the compounds but glucose. The effect of T4 on the excretion of 17-KS and uric acid resembled that of AITC and TU, thus showing that these compounds depressed the androgenic function of the animal."}
{"id": "PMID:466579", "title": "Apparent volume of the biliary tree in the dog.", "content": "The apparent volume of the biliary tree (ABV) in the dog was determined by measuring the mean biliary transit time of injected [14C]taurocholate ([14C]TC). After bolus injection of [14C]TC, entry of bile salt into the lumen of the biliary tree is signaled by an increase in bile flow. The volume of bile collected at the common duct from onset of choleresis until maximal concentration of 14C radioactivity is reached in bile minus the calculated quantity of bile that contains radioactivity and the cannula volume yields a value for the volume of the biliary tree present just prior to injection of [14C]TC. The mean value for ABV in 19 dogs was 2.49 +/- 0.65 microL/g liver (mean +/- SD).", "contents": "Apparent volume of the biliary tree in the dog. The apparent volume of the biliary tree (ABV) in the dog was determined by measuring the mean biliary transit time of injected [14C]taurocholate ([14C]TC). After bolus injection of [14C]TC, entry of bile salt into the lumen of the biliary tree is signaled by an increase in bile flow. The volume of bile collected at the common duct from onset of choleresis until maximal concentration of 14C radioactivity is reached in bile minus the calculated quantity of bile that contains radioactivity and the cannula volume yields a value for the volume of the biliary tree present just prior to injection of [14C]TC. The mean value for ABV in 19 dogs was 2.49 +/- 0.65 microL/g liver (mean +/- SD)."}
{"id": "PMID:466580", "title": "Factors affecting the encapsulation of drugs within liposomes.", "content": "The reported efficiencies of drug encapsulation into liposomes range from less than 0.1% to more than 10% per micromole phospholipid, depending on the nature of the drug and of the liposome employed. We have sought to investigate some of the factors which control the efficiency of drug encapsulation. We have found that most polar drugs are sequestered within the internal aqueous compartment of the liposomes, while nonpolar drugs can bind to the liposome membrane in addition to being sequestered, thus accounting for their higher efficiencies of encapsulation. The encapsulation of nonpolar drugs, but not of polar drugs, is very sensitive to the physical characteristics of the liposome membrane; in particular, a fluid membrane favors the efficient encapsulation of nonpolar compounds. The drug cytosine arabinoside is anomalous in that this highly polar compound seems to interact with the liposome membrane at physiological conditions of pH and ionic strength, thus allowing it to be encapsulated with high efficiency.", "contents": "Factors affecting the encapsulation of drugs within liposomes. The reported efficiencies of drug encapsulation into liposomes range from less than 0.1% to more than 10% per micromole phospholipid, depending on the nature of the drug and of the liposome employed. We have sought to investigate some of the factors which control the efficiency of drug encapsulation. We have found that most polar drugs are sequestered within the internal aqueous compartment of the liposomes, while nonpolar drugs can bind to the liposome membrane in addition to being sequestered, thus accounting for their higher efficiencies of encapsulation. The encapsulation of nonpolar drugs, but not of polar drugs, is very sensitive to the physical characteristics of the liposome membrane; in particular, a fluid membrane favors the efficient encapsulation of nonpolar compounds. The drug cytosine arabinoside is anomalous in that this highly polar compound seems to interact with the liposome membrane at physiological conditions of pH and ionic strength, thus allowing it to be encapsulated with high efficiency."}
{"id": "PMID:466591", "title": "Clinical use of blood, blood components and blood products.", "content": "The goal of modern transfusion therapy is to provide appropriate replacement therapy with blood components as opposed to whole blood for patients with specific hematologic deficiencies. A prerequisite of component therapy is, therefore, correct identification of the deficiency. Appropriate use of components avoids many of the hazards associated with the use of whole blood, and at the same time makes maximal use of this valuable resource. Blood components separated from whole blood soon after collection and appropriately stored can, in combination, provide all the factors present in fresh whole blood. Red cell concentrates prepared from multiple packs have a hematocrit of approximately 70%. They may be stored for up to 3 weeks at 4 degrees C and are recommended for most situations requiring red cell transfusions. Platelet concentrates, which can be stored for up to 72 hours at 22 degrees C, may be used for thrombocytopenic patients. Fresh frozen plasma, stored plasma, cryoprecipitated factor VIII, factor VIII concentrate and factor IX complex concentrate are available for the proper treatment of patients with hemorrhagic disorders due to coagulation factor deficiencies. Similarly, albumin and immune serum globulin are available for their oncotic and antibody properties respectively. Thus, the availability and appropriate use of the various blood products allows not only optimal transfusion therapy for each patient, but also fuller utilization of national blood resources.", "contents": "Clinical use of blood, blood components and blood products. The goal of modern transfusion therapy is to provide appropriate replacement therapy with blood components as opposed to whole blood for patients with specific hematologic deficiencies. A prerequisite of component therapy is, therefore, correct identification of the deficiency. Appropriate use of components avoids many of the hazards associated with the use of whole blood, and at the same time makes maximal use of this valuable resource. Blood components separated from whole blood soon after collection and appropriately stored can, in combination, provide all the factors present in fresh whole blood. Red cell concentrates prepared from multiple packs have a hematocrit of approximately 70%. They may be stored for up to 3 weeks at 4 degrees C and are recommended for most situations requiring red cell transfusions. Platelet concentrates, which can be stored for up to 72 hours at 22 degrees C, may be used for thrombocytopenic patients. Fresh frozen plasma, stored plasma, cryoprecipitated factor VIII, factor VIII concentrate and factor IX complex concentrate are available for the proper treatment of patients with hemorrhagic disorders due to coagulation factor deficiencies. Similarly, albumin and immune serum globulin are available for their oncotic and antibody properties respectively. Thus, the availability and appropriate use of the various blood products allows not only optimal transfusion therapy for each patient, but also fuller utilization of national blood resources."}
{"id": "PMID:466592", "title": "Oral administration of BCG as an adjuvant to surgical treatment of carcinoma of the bronchus.", "content": "A controlled clinical trial of the value of bacille Calmette--Gu\u00e9rin (BCG) vaccine given orally to patients with resectable carcinoma of the lung was conducted in 18 centres across Canada. A total of 308 patients were included in the analysis, 155 in the BCG group and 153 in the control group. The two groups were similar at the time of admission to the trial. BCG (120 mg) was given orally at weekly intervals for 1 month, every 2 weeks up to 3 months and then every 3 months until the total duration of therapy was 18 months. Over a 3- to 5-year follow-up period after the operation there was no difference in survival between the two groups, the proportion alive at 2 years being 61% in the BCG group and 58% in the control group. There was also no evidence of differences in the time to the detection of recurrent or metastatic disease or in the distribution of such disease. An analysis of prognostic factors confirmed the poor survival associated with histologically confirmed lymph node involvement. It may be concluded that no favourable effect from the oral administration of BCG was demonstrated.", "contents": "Oral administration of BCG as an adjuvant to surgical treatment of carcinoma of the bronchus. A controlled clinical trial of the value of bacille Calmette--Gu\u00e9rin (BCG) vaccine given orally to patients with resectable carcinoma of the lung was conducted in 18 centres across Canada. A total of 308 patients were included in the analysis, 155 in the BCG group and 153 in the control group. The two groups were similar at the time of admission to the trial. BCG (120 mg) was given orally at weekly intervals for 1 month, every 2 weeks up to 3 months and then every 3 months until the total duration of therapy was 18 months. Over a 3- to 5-year follow-up period after the operation there was no difference in survival between the two groups, the proportion alive at 2 years being 61% in the BCG group and 58% in the control group. There was also no evidence of differences in the time to the detection of recurrent or metastatic disease or in the distribution of such disease. An analysis of prognostic factors confirmed the poor survival associated with histologically confirmed lymph node involvement. It may be concluded that no favourable effect from the oral administration of BCG was demonstrated."}
{"id": "PMID:466603", "title": "Dietary fibre and colonic neoplasia.", "content": "Dietary plant fibre, or plantix, is thought to play a significant role in the pathogenesis of colon cancer in humans. It is a complex polymeric substance that has several distinct components resistant to hydrolysis by the digestive enzymes of humans. These components include cellulose, hemicelluloses, pectins, lignin, gums, mucilages and, in certain instances, algal polysaccharides. These polymers have different physicochemical properties, and recent evidence from experimental studies in animals treated with carcinogens suggests that some may exert protective effects in the intestine and others may enhance colon carcinogenesis. This review synthesizes information on the chemical composition, methods of analysis and physicochemical properties of dietary plant fibre and reviews available studies examining the role of fibre in colonic neoplasia in animals and humans.", "contents": "Dietary fibre and colonic neoplasia. Dietary plant fibre, or plantix, is thought to play a significant role in the pathogenesis of colon cancer in humans. It is a complex polymeric substance that has several distinct components resistant to hydrolysis by the digestive enzymes of humans. These components include cellulose, hemicelluloses, pectins, lignin, gums, mucilages and, in certain instances, algal polysaccharides. These polymers have different physicochemical properties, and recent evidence from experimental studies in animals treated with carcinogens suggests that some may exert protective effects in the intestine and others may enhance colon carcinogenesis. This review synthesizes information on the chemical composition, methods of analysis and physicochemical properties of dietary plant fibre and reviews available studies examining the role of fibre in colonic neoplasia in animals and humans."}
{"id": "PMID:466616", "title": "Report of a clinical trial of a Canadian preparation of antihemophilic factor.", "content": "A clinical trial of a Canadian preparation of antihemophilic factor has been completed. The mean percentage increase in the factor VIII coagulant activity in the plasma was determined to be 2.1 +/- 0.8 per unit per kilogram dose, which compares very favourably with published data. The mean half-life of antihemophilic factor was found to be 14.7 hours. Side effects and reactions to the product were minimal and the product has been rated as clinically effective. No significant differences between lots were noted in the in vivo response or the half-life.", "contents": "Report of a clinical trial of a Canadian preparation of antihemophilic factor. A clinical trial of a Canadian preparation of antihemophilic factor has been completed. The mean percentage increase in the factor VIII coagulant activity in the plasma was determined to be 2.1 +/- 0.8 per unit per kilogram dose, which compares very favourably with published data. The mean half-life of antihemophilic factor was found to be 14.7 hours. Side effects and reactions to the product were minimal and the product has been rated as clinically effective. No significant differences between lots were noted in the in vivo response or the half-life."}
{"id": "PMID:466617", "title": "Familial hypogonadotropic hypogonadism with alopecia.", "content": "In one family several male and female members had hypogonadism and frontoparietal alopecia, whereas other members with normal sexual development had normal scalp hair. Clinical and laboratory evaluation of three affected young men (two brothers and their cousin) revealed that the hypogonadism was the result of decreased serum concentrations of follicle stimulating and luteinizing hormones. There was no evidence of a deficiency of any other pituitary hormone. Long-term treatment of the three patients with human chorionic gonadotropin resulted in an increase in the serum testosterone concentration, the appearance of male secondary sex characteristics and an increase in the size of the external genitalia.", "contents": "Familial hypogonadotropic hypogonadism with alopecia. In one family several male and female members had hypogonadism and frontoparietal alopecia, whereas other members with normal sexual development had normal scalp hair. Clinical and laboratory evaluation of three affected young men (two brothers and their cousin) revealed that the hypogonadism was the result of decreased serum concentrations of follicle stimulating and luteinizing hormones. There was no evidence of a deficiency of any other pituitary hormone. Long-term treatment of the three patients with human chorionic gonadotropin resulted in an increase in the serum testosterone concentration, the appearance of male secondary sex characteristics and an increase in the size of the external genitalia."}
{"id": "PMID:466623", "title": "The Canadian certification examination in psychiatry. II: Who passes and who fails.", "content": "No analysis of Canadian certification examinations in psychiatry has previously been published although analyses of the American Board of Psychiatry and Neurology and the British Membership examinations are available. Because candidates, directors of residency training, mental health planners and consumers are all interested in who passes and who fails the certification examinations, available examination data for English speaking candidates are analyzed. Successful candidates are more likely to be younger; to have attended medical schools in English speaking countries (in North America, the British Isles, or the \"old Dominions\"); to have placed in the upper two-thirds of their medical school class; to have entered psychiatric training soon after graduating from medical school and then to have completed their training without interruption. Some limitations of the examinations and the problem of candidates who fail are briefly discussed.", "contents": "The Canadian certification examination in psychiatry. II: Who passes and who fails. No analysis of Canadian certification examinations in psychiatry has previously been published although analyses of the American Board of Psychiatry and Neurology and the British Membership examinations are available. Because candidates, directors of residency training, mental health planners and consumers are all interested in who passes and who fails the certification examinations, available examination data for English speaking candidates are analyzed. Successful candidates are more likely to be younger; to have attended medical schools in English speaking countries (in North America, the British Isles, or the \"old Dominions\"); to have placed in the upper two-thirds of their medical school class; to have entered psychiatric training soon after graduating from medical school and then to have completed their training without interruption. Some limitations of the examinations and the problem of candidates who fail are briefly discussed."}
{"id": "PMID:466624", "title": "The Canadian certification examination in psychiatry. III. Towards better certification techniques.", "content": "Although there has been much criticism of specialty certification examinations there is general agreement that they are an important safeguard of competence in medical specialties where the consumer cannot judge this. The Canadian Royal College has made strenuous attempts to improve certification techniques but these have not been heretofore widely reported. This paper reports on the rationale for the replacement of the essay examination by multiple choice questionnaires, the problems associated with MCQ, and the efforts made to improve the fairness, validity and reliability of the clinical (oral) examination. These efforts have been in the areas of selection of examiners, training for examiners, standardization of marking and the conduct of the examination. A description is given of the conduct of the examination and what the examiners look for in the candidate's performance. The development and increasing importance of the In-Training Evaluation are discussed. The publication of A Resident's Guide to Psychiatric Education with multinational participation advances the possibility of future reciprocity in psychiatric examinations conducted in several English speaking countries.", "contents": "The Canadian certification examination in psychiatry. III. Towards better certification techniques. Although there has been much criticism of specialty certification examinations there is general agreement that they are an important safeguard of competence in medical specialties where the consumer cannot judge this. The Canadian Royal College has made strenuous attempts to improve certification techniques but these have not been heretofore widely reported. This paper reports on the rationale for the replacement of the essay examination by multiple choice questionnaires, the problems associated with MCQ, and the efforts made to improve the fairness, validity and reliability of the clinical (oral) examination. These efforts have been in the areas of selection of examiners, training for examiners, standardization of marking and the conduct of the examination. A description is given of the conduct of the examination and what the examiners look for in the candidate's performance. The development and increasing importance of the In-Training Evaluation are discussed. The publication of A Resident's Guide to Psychiatric Education with multinational participation advances the possibility of future reciprocity in psychiatric examinations conducted in several English speaking countries."}
{"id": "PMID:466626", "title": "The relationship between agoraphobia and primary affective disorders.", "content": "Detailed clinical records were kept on a series of 55 agoraphobic patients who presented to a general hospital psychiatric practice over a 3 year period. A review of the records revealed that 91% of these patients were diagnosed as suffering from a unipolar or bipolar primary affective disorder. The anxiety and phobic symptoms tended to mask the presence of the affective disorder. This observation is consistent with most of the published data on the agoraphobic syndrome. It could also explain the inconsistent effects of treatment of agoraphobia compared with simple phobias. The possible biological and psychological connections between primary affective disorders and the agoraphobic syndrome are discussed.", "contents": "The relationship between agoraphobia and primary affective disorders. Detailed clinical records were kept on a series of 55 agoraphobic patients who presented to a general hospital psychiatric practice over a 3 year period. A review of the records revealed that 91% of these patients were diagnosed as suffering from a unipolar or bipolar primary affective disorder. The anxiety and phobic symptoms tended to mask the presence of the affective disorder. This observation is consistent with most of the published data on the agoraphobic syndrome. It could also explain the inconsistent effects of treatment of agoraphobia compared with simple phobias. The possible biological and psychological connections between primary affective disorders and the agoraphobic syndrome are discussed."}
{"id": "PMID:466627", "title": "The limits of Munchausen's syndrome.", "content": "The authors review the literature on Munchausen's syndrome and speculate about possible underlying psychological mechanisms. The proposed DSM III classification of factitious illnesses suggests a continuum from hysteria on one end of the spectrum to malingering on the other. Two case studies are presented which represent variants of this syndrome. Both patients were given a sodium amytal interview, a procedure not previously reported in the Munchausen's literature. The procedure was helpful in eliciting a more accurate history and a clearer sense of the underlying dynamics. Some suggestions for further research are made.", "contents": "The limits of Munchausen's syndrome. The authors review the literature on Munchausen's syndrome and speculate about possible underlying psychological mechanisms. The proposed DSM III classification of factitious illnesses suggests a continuum from hysteria on one end of the spectrum to malingering on the other. Two case studies are presented which represent variants of this syndrome. Both patients were given a sodium amytal interview, a procedure not previously reported in the Munchausen's literature. The procedure was helpful in eliciting a more accurate history and a clearer sense of the underlying dynamics. Some suggestions for further research are made."}
{"id": "PMID:466628", "title": "Civil commitment practices in 1977: troubled semantics and/or troubled psychiatry.", "content": "This paper examines the practice of involuntary mental hospitalization through examination of criteria used for committment in a sample of 200 civil commitment certificates. Special reference is made, following previous research, to criteria used to describe the person deemed dangerous to himself or to others. The relevance of the findings of present practices in mental health is discussed. New procedures which facilitate presentation of factual evidence, and which eliminate gratuitous information, are required without delay.", "contents": "Civil commitment practices in 1977: troubled semantics and/or troubled psychiatry. This paper examines the practice of involuntary mental hospitalization through examination of criteria used for committment in a sample of 200 civil commitment certificates. Special reference is made, following previous research, to criteria used to describe the person deemed dangerous to himself or to others. The relevance of the findings of present practices in mental health is discussed. New procedures which facilitate presentation of factual evidence, and which eliminate gratuitous information, are required without delay."}
{"id": "PMID:466629", "title": "Length of hospitalization predicted by self assessment of social competence.", "content": "The purpose of the study was to assess the feasibility of using demographic and social competence information to predict length of stay in a psychiatric hospital. The results indicated that social competence variables were much better predictors of length of stay than demographic variables. Moreover, no combination of demographic and social competence variables led to more accurate predictors than social competence information alone.", "contents": "Length of hospitalization predicted by self assessment of social competence. The purpose of the study was to assess the feasibility of using demographic and social competence information to predict length of stay in a psychiatric hospital. The results indicated that social competence variables were much better predictors of length of stay than demographic variables. Moreover, no combination of demographic and social competence variables led to more accurate predictors than social competence information alone."}
{"id": "PMID:466630", "title": "The psychoanalytic theory of depression. Part I--The major contributors.", "content": "This article is the first of a two part review of the psychoanalytic theory of depression. In this first part of the review the work of the major contributors to the psychoanalytic theory of depression is discussed. The writings of Abraham, Freud, Rado, Klein, Jacobson, Benedek, Bibring, Spitz, Sandler and Bowlby, among other, are presented and critically reviewed by the author. The work of these authors has been selected for this review because they have made the most seminal contributions to the development of the psychoanalytic theory of depression. Necessarily those authors whose contributions have been largely clinical have not been included, the major focus of this review being theoretical. In reviewing the writings of the major contributions the major themes in the theory of depression can be discussed. These themes will form the subject of the next paper in this review.", "contents": "The psychoanalytic theory of depression. Part I--The major contributors. This article is the first of a two part review of the psychoanalytic theory of depression. In this first part of the review the work of the major contributors to the psychoanalytic theory of depression is discussed. The writings of Abraham, Freud, Rado, Klein, Jacobson, Benedek, Bibring, Spitz, Sandler and Bowlby, among other, are presented and critically reviewed by the author. The work of these authors has been selected for this review because they have made the most seminal contributions to the development of the psychoanalytic theory of depression. Necessarily those authors whose contributions have been largely clinical have not been included, the major focus of this review being theoretical. In reviewing the writings of the major contributions the major themes in the theory of depression can be discussed. These themes will form the subject of the next paper in this review."}
{"id": "PMID:466637", "title": "Induced parturition in swine herds.", "content": "Intramuscular injections of various levels of prostaglandin F(2alpha) were administered to 116 pregnant swine in three commercial herds and the University research unit on Day 111, 112 or 113 of gestation. Sixty-three percent of the treated animals farrowed during the working day immediately following treatment. The parturition time, birth weight, litter size, number of piglets weaned, growth of the piglets to weaning and subsequent rebreeding of the dams were comparable with the expected performance in the herds. The results indicated that prostaglandin F(2alpha) in doses ranging from 5 to 12.5 mg per animal were effective for induction of parturition in a substantial proportion of the treated animals. Successful induction of parturition could reduce the farrowing interval for batches, allow more supervision of farrowing, facilitate transfer of piglets from large to small litters and generally increase efficiency in the farrowing unit. This technique could have practical application in intensive swine production units.", "contents": "Induced parturition in swine herds. Intramuscular injections of various levels of prostaglandin F(2alpha) were administered to 116 pregnant swine in three commercial herds and the University research unit on Day 111, 112 or 113 of gestation. Sixty-three percent of the treated animals farrowed during the working day immediately following treatment. The parturition time, birth weight, litter size, number of piglets weaned, growth of the piglets to weaning and subsequent rebreeding of the dams were comparable with the expected performance in the herds. The results indicated that prostaglandin F(2alpha) in doses ranging from 5 to 12.5 mg per animal were effective for induction of parturition in a substantial proportion of the treated animals. Successful induction of parturition could reduce the farrowing interval for batches, allow more supervision of farrowing, facilitate transfer of piglets from large to small litters and generally increase efficiency in the farrowing unit. This technique could have practical application in intensive swine production units."}
{"id": "PMID:466638", "title": "Torsion of the uterus--a cause of colic in the mare.", "content": "A case of uterine torsion in a mare with colic is described in which an early diagnosis was made and the torsion successfully reduced through a standing laparotomy with survival of both the mare and the foal. This case is used to stress the importance of thorough examination of all pregnant mares with colic in order to differentiate uterine torsion from other causes of abdominal pain, thereby avoiding delay in surgical correction and reducing the risk of fetal and/or maternal death. The advantages of the standing laparotomy are presented in support of this method of treatment of torsion of the gravid uterus.", "contents": "Torsion of the uterus--a cause of colic in the mare. A case of uterine torsion in a mare with colic is described in which an early diagnosis was made and the torsion successfully reduced through a standing laparotomy with survival of both the mare and the foal. This case is used to stress the importance of thorough examination of all pregnant mares with colic in order to differentiate uterine torsion from other causes of abdominal pain, thereby avoiding delay in surgical correction and reducing the risk of fetal and/or maternal death. The advantages of the standing laparotomy are presented in support of this method of treatment of torsion of the gravid uterus."}
{"id": "PMID:466641", "title": "Amelioration of adriamycin skin necrosis: an experimental study.", "content": "Skin necrosis following subcutaneous Adriamycin extravasation is a significant clinical problem. In this study Adriamycin dilution and various drugs were tested for their ability to ameliorate this toxic effect. When Adriamycin was diluted to a concentration of less than or equal to 0.25 mg/ml, no skin ulceration was observed. Drugs such as hydrocortisone, dexamethasone, lidocaine, bupivacaine, phentolamine, and hyaluronidase were ineffective in modifying Adriamycin skin necrosis. Care in iv drug administration and dilution of Adriamycin are the best means of preventing the sequelae of subcutaneous Adriamycin extravasation.", "contents": "Amelioration of adriamycin skin necrosis: an experimental study. Skin necrosis following subcutaneous Adriamycin extravasation is a significant clinical problem. In this study Adriamycin dilution and various drugs were tested for their ability to ameliorate this toxic effect. When Adriamycin was diluted to a concentration of less than or equal to 0.25 mg/ml, no skin ulceration was observed. Drugs such as hydrocortisone, dexamethasone, lidocaine, bupivacaine, phentolamine, and hyaluronidase were ineffective in modifying Adriamycin skin necrosis. Care in iv drug administration and dilution of Adriamycin are the best means of preventing the sequelae of subcutaneous Adriamycin extravasation."}
{"id": "PMID:466644", "title": "Influence of L-tyrosine phenol-lyase on the growth and metabolism of B16 melanoma.", "content": "Administration of L-tyrosine phenol-lyase inhibited growth of established B16 melanomas in BDF1 mice. Under the conditions used, the enzyme exerted a cytostatic effect on the tumors. The addition of L-tyrosine phenol-lyase to growing melanoma cultures blocked cell division and caused an accumulation of cells in the G1 (G0) pultures was suppressed, the most pronounced effect of L-tyrosine phenol-lyase was a rapid and near-complete cessation of uridine uptake and incorporation. This block in RNA synthesis was also observed in human fibroblasts treated with L-tyrosine phenol-lyase.", "contents": "Influence of L-tyrosine phenol-lyase on the growth and metabolism of B16 melanoma. Administration of L-tyrosine phenol-lyase inhibited growth of established B16 melanomas in BDF1 mice. Under the conditions used, the enzyme exerted a cytostatic effect on the tumors. The addition of L-tyrosine phenol-lyase to growing melanoma cultures blocked cell division and caused an accumulation of cells in the G1 (G0) pultures was suppressed, the most pronounced effect of L-tyrosine phenol-lyase was a rapid and near-complete cessation of uridine uptake and incorporation. This block in RNA synthesis was also observed in human fibroblasts treated with L-tyrosine phenol-lyase."}
{"id": "PMID:466645", "title": "Biochemical properties and immunogenicity of L-phenylalanine ammonia-lyase: effects on tumor-bearing mice.", "content": "L-Phenylalanine ammonia-lyase (PAL) from yeast was used to deplete plasma L-phenylalanine and L-tyrosine in an attempt to achieve inhibition of tumor growth in mice. Plasma L-phenylalanine and L-tyrosine were reduced to nondetectable levels when circulating PAL activity was maintained at greater than or equal to 0.06 unit/ml. Repeated administration resulted in the appearance of anti-PAL antibodies. A radioimmunoassay based on the method of Farr was developed to determine quantitatively the presence of anti-PAL. Sublethal total-body irradiation temporarily suppressed the immunologic response of the host. Long-term specific immunosuppression to PAL was achieved with cyclophosphamide (CPA). A single dose of 180 mg/kg of CPA administered ip to mice 24 hours before, simultaneously with, or 24 hours after 100 units/kg of PAL induced tolerance for 450 days (20 injections of enzyme). The plasma half-life of PAL in CPA-treated mice remained essentially the same as that found after a single injection (25 hours), and anti-PAL probably will require specific immunosuppression of the host to repeated injections of the enzyme.", "contents": "Biochemical properties and immunogenicity of L-phenylalanine ammonia-lyase: effects on tumor-bearing mice. L-Phenylalanine ammonia-lyase (PAL) from yeast was used to deplete plasma L-phenylalanine and L-tyrosine in an attempt to achieve inhibition of tumor growth in mice. Plasma L-phenylalanine and L-tyrosine were reduced to nondetectable levels when circulating PAL activity was maintained at greater than or equal to 0.06 unit/ml. Repeated administration resulted in the appearance of anti-PAL antibodies. A radioimmunoassay based on the method of Farr was developed to determine quantitatively the presence of anti-PAL. Sublethal total-body irradiation temporarily suppressed the immunologic response of the host. Long-term specific immunosuppression to PAL was achieved with cyclophosphamide (CPA). A single dose of 180 mg/kg of CPA administered ip to mice 24 hours before, simultaneously with, or 24 hours after 100 units/kg of PAL induced tolerance for 450 days (20 injections of enzyme). The plasma half-life of PAL in CPA-treated mice remained essentially the same as that found after a single injection (25 hours), and anti-PAL probably will require specific immunosuppression of the host to repeated injections of the enzyme."}
{"id": "PMID:466646", "title": "Tumor therapy by deprivation of L-methionine: rationale and results.", "content": "Published reports indicate that normal rodent cells can grow in medium containing either L-methionine or L-homocysteine, whereas malignant rodent cells have an absolute requirement for L-methionine. Our studies with two normal human cell lines (fetal lung fibroblasts and bladder epithelial cells) exhibit equal growth in media containing either L-methionine or L-homocysteine. The same is true for five malignant human cell lines (carcinoma of the cervix [HeLa], adenocarcinoma of the breast [AlAb], acute lymphoblastic leukemia [MOLT-3], Wilms' tumor [SK-NEP-1], and reticulum cell sarcoma [T-77], whereas four other malignant cell lines (adenocarcinoma of the breast [SK-BR-2-III], the two lymphoblastic leukemias [CCRF-HSB-2 and CCRF-SB], and a neuroblastoma [SK-N-MC]) have absolute requirements for L-methionine. Two malignant cell lines, an adenocarcinoma of the lung (A549) and an adenocarcinoma of the pancreas (Capan-1), showed restricted growth under the experimental conditions used. L-Methionlinase (L-methionine-alpha-deamino-gamma-mercaptomethane-lyase, EC 4.4.1.11) at a concentration of 0.1 unit/ml leads to complete growth inhibition of cell cultures of both the normal human fetal lung fibroblasts (F-136-35-56) and the acute lymphoblastic leukemia (CCRF-HSB-2). L-Homocysteine-thiolactone in medium containing L-methioninase could partly \"rescue\" the normal but not the malignant cells.", "contents": "Tumor therapy by deprivation of L-methionine: rationale and results. Published reports indicate that normal rodent cells can grow in medium containing either L-methionine or L-homocysteine, whereas malignant rodent cells have an absolute requirement for L-methionine. Our studies with two normal human cell lines (fetal lung fibroblasts and bladder epithelial cells) exhibit equal growth in media containing either L-methionine or L-homocysteine. The same is true for five malignant human cell lines (carcinoma of the cervix [HeLa], adenocarcinoma of the breast [AlAb], acute lymphoblastic leukemia [MOLT-3], Wilms' tumor [SK-NEP-1], and reticulum cell sarcoma [T-77], whereas four other malignant cell lines (adenocarcinoma of the breast [SK-BR-2-III], the two lymphoblastic leukemias [CCRF-HSB-2 and CCRF-SB], and a neuroblastoma [SK-N-MC]) have absolute requirements for L-methionine. Two malignant cell lines, an adenocarcinoma of the lung (A549) and an adenocarcinoma of the pancreas (Capan-1), showed restricted growth under the experimental conditions used. L-Methionlinase (L-methionine-alpha-deamino-gamma-mercaptomethane-lyase, EC 4.4.1.11) at a concentration of 0.1 unit/ml leads to complete growth inhibition of cell cultures of both the normal human fetal lung fibroblasts (F-136-35-56) and the acute lymphoblastic leukemia (CCRF-HSB-2). L-Homocysteine-thiolactone in medium containing L-methioninase could partly \"rescue\" the normal but not the malignant cells."}
{"id": "PMID:466647", "title": "L-cyst(e)ine requirements of malignant cells and progress toward depletion therapy.", "content": "The L-cyst(e)ine requirements of normal and malignant cells are reviewed and expanded within the context of establishing whether the measurement of gamma-cystathionase levels constitutes a predictive test for tumor sensitivity to L-cyst(e)ine depletion. The ability of both purified L-cysteine desulfhydrase and gamma-cystathionase to inhibit the growth of the L-cystine-dependent L1210 leukemia in culture is presented, as well as approaches to circumvent the limitations of these enzymes for in vivo therapy. The ability of proparagylglycine to inhibit L-cysteine biosynthesis in vivo is reviewed for its possible use in combination therapy. In addition, the ability of poly D,L-alanine modification of Escherichia coli L-asparaginase to increase the plasma half-life in mice tenfold as well as to decrease the immunogenicity of the enzyme is presented.", "contents": "L-cyst(e)ine requirements of malignant cells and progress toward depletion therapy. The L-cyst(e)ine requirements of normal and malignant cells are reviewed and expanded within the context of establishing whether the measurement of gamma-cystathionase levels constitutes a predictive test for tumor sensitivity to L-cyst(e)ine depletion. The ability of both purified L-cysteine desulfhydrase and gamma-cystathionase to inhibit the growth of the L-cystine-dependent L1210 leukemia in culture is presented, as well as approaches to circumvent the limitations of these enzymes for in vivo therapy. The ability of proparagylglycine to inhibit L-cysteine biosynthesis in vivo is reviewed for its possible use in combination therapy. In addition, the ability of poly D,L-alanine modification of Escherichia coli L-asparaginase to increase the plasma half-life in mice tenfold as well as to decrease the immunogenicity of the enzyme is presented."}
{"id": "PMID:466649", "title": "L-Threonine deaminase as a possible antitumor agent.", "content": "In vitro studies with L-threonine deaminase purified from sheep liver demonstrated that the enzyme was cytotoxic toward two lines of murine leukemia cells but not toward L cells or normal human fibroblasts. When L-threonine deaminase was injected into mice, it was possible to lower the plasma L-threonine concentration by 90%. The enzyme had a half-life of 1.5 hours in the circulation.", "contents": "L-Threonine deaminase as a possible antitumor agent. In vitro studies with L-threonine deaminase purified from sheep liver demonstrated that the enzyme was cytotoxic toward two lines of murine leukemia cells but not toward L cells or normal human fibroblasts. When L-threonine deaminase was injected into mice, it was possible to lower the plasma L-threonine concentration by 90%. The enzyme had a half-life of 1.5 hours in the circulation."}
{"id": "PMID:466650", "title": "Enhanced effect of an L-glutamine antagonist, L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid, by Acinetobacter L-glutaminase-L-asparaginase.", "content": "The effect of L-glutamine and L-asparagine depletion by Acinetobacter L-glutaminase-L-asparaginase on the toxicity and antitumor activity of L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (NSC-163501) was tested in mice. The LD50 of six daily doses of NSC-163501 in BDF1 female mice decreased from 7.5 to 0.3 mg/kg/day by combination treatment with the enzyme. Enzyme therapy also decreased the dose of NSC-163501 needed for maximal prolongation of survival in these mice inoculated with L1210 leukemia. Nevertheless, the combination did not prolong survival in L1210-bearing mice beyond that of higher doses of NSC-163501 alone. In contrast, the combination of enzyme plus NSC-163501 inhibited the growth of established sc implanted Ehrlich ascites carcinoma in ICRf male mice much more than either agent alone. Treatment with Acinetobacter L-glutaminase-L-asparaginase decreased the L-asparagine and L-glutamine levels in acid extracts of the Ehrlich tumor. NSC-163501 did not affect the amide levels or alter the decrease produced by enzyme therapy.", "contents": "Enhanced effect of an L-glutamine antagonist, L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid, by Acinetobacter L-glutaminase-L-asparaginase. The effect of L-glutamine and L-asparagine depletion by Acinetobacter L-glutaminase-L-asparaginase on the toxicity and antitumor activity of L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (NSC-163501) was tested in mice. The LD50 of six daily doses of NSC-163501 in BDF1 female mice decreased from 7.5 to 0.3 mg/kg/day by combination treatment with the enzyme. Enzyme therapy also decreased the dose of NSC-163501 needed for maximal prolongation of survival in these mice inoculated with L1210 leukemia. Nevertheless, the combination did not prolong survival in L1210-bearing mice beyond that of higher doses of NSC-163501 alone. In contrast, the combination of enzyme plus NSC-163501 inhibited the growth of established sc implanted Ehrlich ascites carcinoma in ICRf male mice much more than either agent alone. Treatment with Acinetobacter L-glutaminase-L-asparaginase decreased the L-asparagine and L-glutamine levels in acid extracts of the Ehrlich tumor. NSC-163501 did not affect the amide levels or alter the decrease produced by enzyme therapy."}
{"id": "PMID:466651", "title": "Treatment of L5178Y tumor-bearing BDF1 mice with a nonimmunogenic L-glutaminase-L-asparaginase.", "content": "An L-glutaminase-L-asparaginase from Achromobacter has been rendered nonimmunogenic by the covalent attachment of polyethylene glycol (PEG) to nonessential amine groups of the enzyme. PEG-L-glutaminase-L-asparaginase exhibits a greatly enhanced half-life in the bloodstream compared to the unmodified enzyme in normal mice, and is effective in prolonging the survival of BDF1 mice inoculated ip with L5178Y cells. PEG-L-glutaminase-L-asparaginase appears rapidly in the blood following ip injection.", "contents": "Treatment of L5178Y tumor-bearing BDF1 mice with a nonimmunogenic L-glutaminase-L-asparaginase. An L-glutaminase-L-asparaginase from Achromobacter has been rendered nonimmunogenic by the covalent attachment of polyethylene glycol (PEG) to nonessential amine groups of the enzyme. PEG-L-glutaminase-L-asparaginase exhibits a greatly enhanced half-life in the bloodstream compared to the unmodified enzyme in normal mice, and is effective in prolonging the survival of BDF1 mice inoculated ip with L5178Y cells. PEG-L-glutaminase-L-asparaginase appears rapidly in the blood following ip injection."}
{"id": "PMID:466652", "title": "In vitro system for detection of antimetabolites of specific amino acids.", "content": "An in vitro system was developed to detect antimetabolites in fermentation liquors of soil microorganisms. This system effectively uncovers antimetabolites of purines and pyrimidines and of selected amino acids with established differences in their biosynthesis by normal cells versus certain malignant cells. Currently these amino acids include: L-asparagine, L-aspartic acid, L-glutamine, L-cysteine (cystine), L-methionine, L-arginine, L-histidine, L-tyrosine, L-phenylalanine, L-tryptophan, L-threonine, and L-serine. It is expected that these antimetabolites could be useful either alone or in combination with specific depleting enzymes in the treatment of malignancies where such an imbalance was established.", "contents": "In vitro system for detection of antimetabolites of specific amino acids. An in vitro system was developed to detect antimetabolites in fermentation liquors of soil microorganisms. This system effectively uncovers antimetabolites of purines and pyrimidines and of selected amino acids with established differences in their biosynthesis by normal cells versus certain malignant cells. Currently these amino acids include: L-asparagine, L-aspartic acid, L-glutamine, L-cysteine (cystine), L-methionine, L-arginine, L-histidine, L-tyrosine, L-phenylalanine, L-tryptophan, L-threonine, and L-serine. It is expected that these antimetabolites could be useful either alone or in combination with specific depleting enzymes in the treatment of malignancies where such an imbalance was established."}
{"id": "PMID:466654", "title": "Effect of a forced diuresis on the distribution and excretion (via urine and bile) of 195mplatinum when given as 195mplatinum cis-dichlorodiammineplatinum(II).", "content": "195mPlatinum cis-dichlorodiammineplatinum(II) (195mPt cis-DDP) was injected iv with furosemide or mannitol into male Sprague-Dawley rats. The effect of such a diuresis on the distribution, excretion, and retention of cis-DDP was measured. The mannitol-treated animals had a significant increase in the urinary excretion of 196mPt over control animals for the first five 15-minute collection times and for the 24-hour cumulative urine for the 0.01-mg/kg dose. At the 6-mg/kg dose, only the first three 15-minute and 24-hour cumulative urine collections were significant. This increased excretion, however, did not affect tissue or blood concentrations of this agent. Furosemide did not affect the excretion of 195mPt. A biliary excretion of 195mPt given as 195mPt cis-DDP is documented. The excretion is biphasic, with a rapid phase followed by a slower protracted phase. Forced diuresis, if it affords renal protection, does so during the first 90 minutes of urinary excretion.", "contents": "Effect of a forced diuresis on the distribution and excretion (via urine and bile) of 195mplatinum when given as 195mplatinum cis-dichlorodiammineplatinum(II). 195mPlatinum cis-dichlorodiammineplatinum(II) (195mPt cis-DDP) was injected iv with furosemide or mannitol into male Sprague-Dawley rats. The effect of such a diuresis on the distribution, excretion, and retention of cis-DDP was measured. The mannitol-treated animals had a significant increase in the urinary excretion of 196mPt over control animals for the first five 15-minute collection times and for the 24-hour cumulative urine for the 0.01-mg/kg dose. At the 6-mg/kg dose, only the first three 15-minute and 24-hour cumulative urine collections were significant. This increased excretion, however, did not affect tissue or blood concentrations of this agent. Furosemide did not affect the excretion of 195mPt. A biliary excretion of 195mPt given as 195mPt cis-DDP is documented. The excretion is biphasic, with a rapid phase followed by a slower protracted phase. Forced diuresis, if it affords renal protection, does so during the first 90 minutes of urinary excretion."}
{"id": "PMID:466655", "title": "Biologic activity of MCNU: a new antitumor agent.", "content": "A novel nitrosourea derivative, methyl-6-[[[(2-chloroethyl)nitrosoamino]carbonyl]-amino]-6-deoxy-alpha-D-glucopyranoside (MCNU), is a water-soluble compound in which a methoxyl group is attached to the C-1 position and an N-(2-chloroethyl)-N-nitrosoureido group is attached to the C-6 position of the glucose moiety. MCNU exhibited a marked life-prolongation or growth-inhibitory effect against mouse L1210 leukemia, adenocarcinoma 755, Nakahara-Fukuoka sarcoma, Lewis lung carcinoma, and B16 melanoma. Ip, oral, or iv administration of MCNU was markedly effective against L1210 leukemia, and the therapeutic ratio by ip administration was larger than that of chlorozotocin or CCNU. The life-prolongation effect of MCNU against established Lewis lung carcinoma was similar to that of methyl-CCNU. The bone marrow toxicity of MCNU was less than that of CCNU but considerably more than that of chlorozotocin.", "contents": "Biologic activity of MCNU: a new antitumor agent. A novel nitrosourea derivative, methyl-6-[[[(2-chloroethyl)nitrosoamino]carbonyl]-amino]-6-deoxy-alpha-D-glucopyranoside (MCNU), is a water-soluble compound in which a methoxyl group is attached to the C-1 position and an N-(2-chloroethyl)-N-nitrosoureido group is attached to the C-6 position of the glucose moiety. MCNU exhibited a marked life-prolongation or growth-inhibitory effect against mouse L1210 leukemia, adenocarcinoma 755, Nakahara-Fukuoka sarcoma, Lewis lung carcinoma, and B16 melanoma. Ip, oral, or iv administration of MCNU was markedly effective against L1210 leukemia, and the therapeutic ratio by ip administration was larger than that of chlorozotocin or CCNU. The life-prolongation effect of MCNU against established Lewis lung carcinoma was similar to that of methyl-CCNU. The bone marrow toxicity of MCNU was less than that of CCNU but considerably more than that of chlorozotocin."}
{"id": "PMID:466656", "title": "Levodopa and dopamine analogs: melanin precursors as antitumor agents in experimental human and murine leukemia.", "content": "L-Dopa methyl ester has been shown to be a novel antitumor agent. Furthermore, the L-dopa analogs, D-dopa, alpha-methyldopa, and dopamine, also exhibit significant antitumor activity in the L1210 and P388 lymphocytic leukemia systems. Structure-activity studies confirmed that the presence of a catechol moiety was essential for activity. Two analogs, 3,4-dihydroxybenzylamine and N-acetyldopamine, which were much less neurotoxic, exhibited the greatest antitumor activity. In vitro, at concentrations from 0.5 to 3.0 mM, there is a rapid and profound inhibition of radiolabeled thymidine incorporation as compared to uridine or leucine incorporation. Continuous exposure of exponentor up to 24 hours resulted in a block of traverse of cells through the cell cycle in G1 coupled with a depletion of cells with a G2 complement of DNA. In vivo, toxicity of these compounds appears to be mediated principally by conversion to dopamine. Similar effects upon thymidine incorporation were observed in human leukemia cells in vitro.", "contents": "Levodopa and dopamine analogs: melanin precursors as antitumor agents in experimental human and murine leukemia. L-Dopa methyl ester has been shown to be a novel antitumor agent. Furthermore, the L-dopa analogs, D-dopa, alpha-methyldopa, and dopamine, also exhibit significant antitumor activity in the L1210 and P388 lymphocytic leukemia systems. Structure-activity studies confirmed that the presence of a catechol moiety was essential for activity. Two analogs, 3,4-dihydroxybenzylamine and N-acetyldopamine, which were much less neurotoxic, exhibited the greatest antitumor activity. In vitro, at concentrations from 0.5 to 3.0 mM, there is a rapid and profound inhibition of radiolabeled thymidine incorporation as compared to uridine or leucine incorporation. Continuous exposure of exponentor up to 24 hours resulted in a block of traverse of cells through the cell cycle in G1 coupled with a depletion of cells with a G2 complement of DNA. In vivo, toxicity of these compounds appears to be mediated principally by conversion to dopamine. Similar effects upon thymidine incorporation were observed in human leukemia cells in vitro."}
{"id": "PMID:466657", "title": "An effective conscious animal model of atrial fibrillation.", "content": "In 11 fully conscious dogs with chronically implanted spicardial electrodes, 50 Hz sine-wave electrical stimulation of the left atrium reliably and repeatedly produced an arrhythmia which was indistinguishable from atrial fibrillation in terms of its ECG appearance, the statistical properties of the ventricular response and responses to a number of pharmacological agents. In five of the 11 preparations this arrhythmia consistently persisted for periods in excess of 10 min following the cessation of stimulation, indicating an intrinsic cardiac basis for the arrhythmia and suggesting that it is true atrial fibrillation. A close correlation between the ventricular response to this arrhythmia and the degree of atrioventricular conductivity, together with the observation that dramatic changes can occur in ventricular responses without corresponding changes in atrial activity, suggest that the ventricular response is mainly a function of the atrio-ventricular conducting system. The experimental model described is useful for the study of both short and long term drug action no atrial fibrillation and for the evaluation of methods used therapeutically in attempts to terminate episodes of paroxysmal atrial fibrillation.", "contents": "An effective conscious animal model of atrial fibrillation. In 11 fully conscious dogs with chronically implanted spicardial electrodes, 50 Hz sine-wave electrical stimulation of the left atrium reliably and repeatedly produced an arrhythmia which was indistinguishable from atrial fibrillation in terms of its ECG appearance, the statistical properties of the ventricular response and responses to a number of pharmacological agents. In five of the 11 preparations this arrhythmia consistently persisted for periods in excess of 10 min following the cessation of stimulation, indicating an intrinsic cardiac basis for the arrhythmia and suggesting that it is true atrial fibrillation. A close correlation between the ventricular response to this arrhythmia and the degree of atrioventricular conductivity, together with the observation that dramatic changes can occur in ventricular responses without corresponding changes in atrial activity, suggest that the ventricular response is mainly a function of the atrio-ventricular conducting system. The experimental model described is useful for the study of both short and long term drug action no atrial fibrillation and for the evaluation of methods used therapeutically in attempts to terminate episodes of paroxysmal atrial fibrillation."}
{"id": "PMID:466659", "title": "Opposing effects of deoxycorticosterone and spironolactone on isoprenaline-induced myocardial infarction.", "content": "Adult, male Sprague-Dawley rats were subjected to massive myocardial infarction with isoprenaline. Some of the animals were treated with the potent mineralocorticoid, deoxycorticosterone, or the anti-mineralocorticoid, spironolactone. Autopsies spaced at sequential time intervals demonstrated that circulating levels of enzymes, eg, CPK, SGOT, SGPT and LDH, lipids, eg, triglycerides, free fatty acids, and cholesterol, glucose, Bun, and corticosterone rose in conjunction with on-going necrosis and became lowered in conjunction with myocardial repair. Body and organ weights, ie, adrenal, thymus, heart, and kidney, underwent dynamic changes commensurate with necrosis and repair. Animals given isoprenaline alone died (52% survival) during the height of their massive infarct and congestive heart failure (Days 3 and 4). Animals treated with DOC showed the most severe congestion but died (36% survival) during the late repair phase (Days 7 and 8). Animals treated with spironoloactone showed the most effective clearance of thoracic fluid and also dies (18% survival) during the late repair phase (Days 7 and 8). These spironolactone-treated animals had large, left ventricular aneurysms with organised, adherent thrombi. It is believed that alterations in electrolyte balance induced by DOC or spironolactone caused changes in adrenocortical function which greatly modified the usual pathophysiological response to myocardial infarction.", "contents": "Opposing effects of deoxycorticosterone and spironolactone on isoprenaline-induced myocardial infarction. Adult, male Sprague-Dawley rats were subjected to massive myocardial infarction with isoprenaline. Some of the animals were treated with the potent mineralocorticoid, deoxycorticosterone, or the anti-mineralocorticoid, spironolactone. Autopsies spaced at sequential time intervals demonstrated that circulating levels of enzymes, eg, CPK, SGOT, SGPT and LDH, lipids, eg, triglycerides, free fatty acids, and cholesterol, glucose, Bun, and corticosterone rose in conjunction with on-going necrosis and became lowered in conjunction with myocardial repair. Body and organ weights, ie, adrenal, thymus, heart, and kidney, underwent dynamic changes commensurate with necrosis and repair. Animals given isoprenaline alone died (52% survival) during the height of their massive infarct and congestive heart failure (Days 3 and 4). Animals treated with DOC showed the most severe congestion but died (36% survival) during the late repair phase (Days 7 and 8). Animals treated with spironoloactone showed the most effective clearance of thoracic fluid and also dies (18% survival) during the late repair phase (Days 7 and 8). These spironolactone-treated animals had large, left ventricular aneurysms with organised, adherent thrombi. It is believed that alterations in electrolyte balance induced by DOC or spironolactone caused changes in adrenocortical function which greatly modified the usual pathophysiological response to myocardial infarction."}
{"id": "PMID:466661", "title": "Temporal changes in endocardial energy metabolism following propranolol and the metabolic basis for protection against isoprenaline cardiotoxicity.", "content": "Propranolol infusions in open-chested, anesthetised dogs increased endocardial levels of ATP and phosphocreatine. It reduced ADP, AMP, glucose-6-phosphate and lactate in this region. Endocardial glycogen was transiently increased. Metabolic perturbations in the endocardium produced by cardiotoxic doses of isoprenaline were prevented or reduced by propranolol pretreatment. Thus, propranolol appears to have a more selective action on endocardial versus midmyocardial or epicardial metabolism.", "contents": "Temporal changes in endocardial energy metabolism following propranolol and the metabolic basis for protection against isoprenaline cardiotoxicity. Propranolol infusions in open-chested, anesthetised dogs increased endocardial levels of ATP and phosphocreatine. It reduced ADP, AMP, glucose-6-phosphate and lactate in this region. Endocardial glycogen was transiently increased. Metabolic perturbations in the endocardium produced by cardiotoxic doses of isoprenaline were prevented or reduced by propranolol pretreatment. Thus, propranolol appears to have a more selective action on endocardial versus midmyocardial or epicardial metabolism."}
{"id": "PMID:466662", "title": "Resistance of contracting myocardium to swelling with hypoxia and glycolytic blockade.", "content": "The interrelationship of myocardial metabolism, performance and tissue hydration was examined in isolated contracting rat, guinea pig and dog myocardium. Myocardial metabolism was altered by blocking aerobic, and both aerobic and anaerobic metabolism. Myocardial water content and distribution were measured in rat myocardium using 3H-inulin and 51Cr-EDTA as extracellular markers. Myocardial hydration was also evaluated by light and electron microscopy. The relative susceptibility of non-contracting slices of rat and guinea pig myocardium and kidney to swelling secondary to these interventions was also explored. Hypoxia resulted in a partially reversible reduction in mechanical function; hypoxia plus glycolytic blockade led to irreversible severe contracture and total loss of tension development. Neither hypoxia nor hypoxia plus glycolytic blockade resulted in increased total tissue or extracellular water in previously contracting preparations or in non-contracting slices of myocardium. On the other hand, there were significant increases in cellular water in similarly treated kidney slices after each intervention. Thus, despite severe, irreversible derangements of mechanical function, myocardium did not swell under conditions which produced swelling in renal cortex.", "contents": "Resistance of contracting myocardium to swelling with hypoxia and glycolytic blockade. The interrelationship of myocardial metabolism, performance and tissue hydration was examined in isolated contracting rat, guinea pig and dog myocardium. Myocardial metabolism was altered by blocking aerobic, and both aerobic and anaerobic metabolism. Myocardial water content and distribution were measured in rat myocardium using 3H-inulin and 51Cr-EDTA as extracellular markers. Myocardial hydration was also evaluated by light and electron microscopy. The relative susceptibility of non-contracting slices of rat and guinea pig myocardium and kidney to swelling secondary to these interventions was also explored. Hypoxia resulted in a partially reversible reduction in mechanical function; hypoxia plus glycolytic blockade led to irreversible severe contracture and total loss of tension development. Neither hypoxia nor hypoxia plus glycolytic blockade resulted in increased total tissue or extracellular water in previously contracting preparations or in non-contracting slices of myocardium. On the other hand, there were significant increases in cellular water in similarly treated kidney slices after each intervention. Thus, despite severe, irreversible derangements of mechanical function, myocardium did not swell under conditions which produced swelling in renal cortex."}
{"id": "PMID:466664", "title": "A system oriented electrocardiographic amplifier.", "content": "A low noise, programmable gain amplifier has been developed for acquiring ECG data from a variety of recording environments. Features include: low noise (4muV peak-to-peak) differential inputs; digitally programmable gains (range 100 to 16000) and output DC offsets (range -3.5 to +4 V) controlled via a serial interface; externally selectable low frequency response (0.04 or 0.08 Hz); and an on-board output monitor multiplexer controlled via a second serial interface.", "contents": "A system oriented electrocardiographic amplifier. A low noise, programmable gain amplifier has been developed for acquiring ECG data from a variety of recording environments. Features include: low noise (4muV peak-to-peak) differential inputs; digitally programmable gains (range 100 to 16000) and output DC offsets (range -3.5 to +4 V) controlled via a serial interface; externally selectable low frequency response (0.04 or 0.08 Hz); and an on-board output monitor multiplexer controlled via a second serial interface."}
{"id": "PMID:466665", "title": "Morphological characteristics of the spermatogonial stem cells in man.", "content": "The present investigation is concerned with establishing morphological criteria of spermatogonial stem cells in man. Testicular biopsies from patients having undergone semicastration for malignant tumors and radio- and chemotherapy for one year following the operation were studied light and electron microscopically. Those spermatogonial types that survived the treatment were regarded as stem cells in view of the fact that the stem cells, in contrast to the more differentiated spermatogonia, are radiation resistant and less sensitive to various noxious agents. In 7 out of 28 cases examined, a small number of spermatogonia was found adjacent to the basement membrane. The majority of these cells show the characteristic features of pale type A spermatogonia, while a few cells may represent variants of this cell type. The dark type A spermatogonia are almost completely eliminated from the seminiferous tubules. A concept is proposed that the stem cells of the human testis may be derived from the pale type A spermatogonia or the variants of this cell type.", "contents": "Morphological characteristics of the spermatogonial stem cells in man. The present investigation is concerned with establishing morphological criteria of spermatogonial stem cells in man. Testicular biopsies from patients having undergone semicastration for malignant tumors and radio- and chemotherapy for one year following the operation were studied light and electron microscopically. Those spermatogonial types that survived the treatment were regarded as stem cells in view of the fact that the stem cells, in contrast to the more differentiated spermatogonia, are radiation resistant and less sensitive to various noxious agents. In 7 out of 28 cases examined, a small number of spermatogonia was found adjacent to the basement membrane. The majority of these cells show the characteristic features of pale type A spermatogonia, while a few cells may represent variants of this cell type. The dark type A spermatogonia are almost completely eliminated from the seminiferous tubules. A concept is proposed that the stem cells of the human testis may be derived from the pale type A spermatogonia or the variants of this cell type."}
{"id": "PMID:466666", "title": "Ultrastructure of the corpus cardiacum and corpus allatum of the house cricket Acheta domesticus.", "content": "The ultrastructure of the corpus cardiacum (CC) and corpus allatum (CA) of the house cricket, Acheta domesticus, is described. Axon profiles within the CC contain neurosecretory granules 160-350 nm in diameter which are indistinguishable from those found in type I neurosecretory cells of the pars intercerebralis and in the nervus corporis cardiaci I. The CC itself contains two cell types: intrinsic neurosecretory cells and glial cells. Intrinsic NSC cytoplasm contains Golgi bodies and electron dense neurosecretory granules 160-350 nm in diameter. Synaptoid configurations with 20-50 nm diameter electron lucent vesicles were observed within axon profiles of the CC. The structure of the CA is relatively uniform with one cell type predominating. Typical CA cells possess large nucleoli, active Golgi complexes, numerous mitochondria, and occassional microtubules. Groups of dark staining cells scattered throughout the CA of some animals were interpreted as evidence of cellular death.", "contents": "Ultrastructure of the corpus cardiacum and corpus allatum of the house cricket Acheta domesticus. The ultrastructure of the corpus cardiacum (CC) and corpus allatum (CA) of the house cricket, Acheta domesticus, is described. Axon profiles within the CC contain neurosecretory granules 160-350 nm in diameter which are indistinguishable from those found in type I neurosecretory cells of the pars intercerebralis and in the nervus corporis cardiaci I. The CC itself contains two cell types: intrinsic neurosecretory cells and glial cells. Intrinsic NSC cytoplasm contains Golgi bodies and electron dense neurosecretory granules 160-350 nm in diameter. Synaptoid configurations with 20-50 nm diameter electron lucent vesicles were observed within axon profiles of the CC. The structure of the CA is relatively uniform with one cell type predominating. Typical CA cells possess large nucleoli, active Golgi complexes, numerous mitochondria, and occassional microtubules. Groups of dark staining cells scattered throughout the CA of some animals were interpreted as evidence of cellular death."}
{"id": "PMID:466667", "title": "Long term increase of mucosal mast cells in the rat induced by administration of compound 48/80.", "content": "Administration of Compound 48/80 to rats for 5 days resulted in an increase of the specific type of mucosal mast cell, while connective tissue mast cells elsewhere were almost completely degranulated. The number of mucosal mast cells increased slowly for another 5 days and then returned to the control level, in an exponential manner. The half life of the newly formed mast cells was calculated to be about 40 days. This value may be taken as an estimate of the half life of mucosal mast cells. These cells, therefore, constitute a fraction of mast cells with rapid turnover. Available evidence indicates that the classical connective tissue mast cell has a very long life span, without significant turnover in terms of cell death and cell renewal. We suggest that the increase of mucosal mast cells is an indirect effect of Compound 48/80, related to its effect on other mast cells and mediated by material(s) released from these cells during the secretory process.", "contents": "Long term increase of mucosal mast cells in the rat induced by administration of compound 48/80. Administration of Compound 48/80 to rats for 5 days resulted in an increase of the specific type of mucosal mast cell, while connective tissue mast cells elsewhere were almost completely degranulated. The number of mucosal mast cells increased slowly for another 5 days and then returned to the control level, in an exponential manner. The half life of the newly formed mast cells was calculated to be about 40 days. This value may be taken as an estimate of the half life of mucosal mast cells. These cells, therefore, constitute a fraction of mast cells with rapid turnover. Available evidence indicates that the classical connective tissue mast cell has a very long life span, without significant turnover in terms of cell death and cell renewal. We suggest that the increase of mucosal mast cells is an indirect effect of Compound 48/80, related to its effect on other mast cells and mediated by material(s) released from these cells during the secretory process."}
{"id": "PMID:466669", "title": "Ultrastructure and function of the labial nephridia and the rectum of Orchesella cincta (L.) (Collembola).", "content": "The collembolan Orchesella cincta possesses a well-developed coelomoduct kidney. The presence of podocytes in the wall of the sacculus and the fact that the epithelium of the nephridial tubule has the ultrastructural characteristics of resorbing cells, indicate that this is an \"ultrafiltration-reabsorption\" kidney. Apparently also the rectum is lined by a reabsorptive epithelium; the cells possess an extensive system of apical and basal infoldings. This view is sustained by the fact that the stereology of the apical channel system varies in animals kept under different moisture conditions. During the intermoult period, both organs are subject to strong morphological changes, which are obviously related to the feeding rhythm.", "contents": "Ultrastructure and function of the labial nephridia and the rectum of Orchesella cincta (L.) (Collembola). The collembolan Orchesella cincta possesses a well-developed coelomoduct kidney. The presence of podocytes in the wall of the sacculus and the fact that the epithelium of the nephridial tubule has the ultrastructural characteristics of resorbing cells, indicate that this is an \"ultrafiltration-reabsorption\" kidney. Apparently also the rectum is lined by a reabsorptive epithelium; the cells possess an extensive system of apical and basal infoldings. This view is sustained by the fact that the stereology of the apical channel system varies in animals kept under different moisture conditions. During the intermoult period, both organs are subject to strong morphological changes, which are obviously related to the feeding rhythm."}
{"id": "PMID:466670", "title": "Morphogenesis of tight junctions in the peritoneal mesothelium of the mouse embryo.", "content": "The peritoneal mesothelium of mouse embryos (12 to 18 day of gestation) was studied by freeze-fracture and in sections in order to reveal the initial formation of the tight junctions. Freeze-fracture observations showed three types of tight junctions. Type I consists of belt-like meshworks of elevations on the P face and of shallow grooves on the E face. No tight junctional particle can be seen either on the elevations or in the grooves. Type II shows rows of discontinuous particles on the elevations on the P face. Type III consists of strands forming ridges on the P face. On the E face, the grooves of Type II and III appear to be narrower and sharper than those of Type I. Quantitatively, Type I junctions are most numerous during the early stages (day 12-13) of embryonic development, while Type III junctions become more common in the later stages, and are the only type seen by day 18. Observations on sections, however, fail to distinguish between the three types. The results suggest that an initial sign of tight junction formation is close apposition of the two cell membranes in the junctional domain, without tight junctional particles. Later, the particles appear to be incorporated in the tight junctions and the strands form by fusion of the particles.", "contents": "Morphogenesis of tight junctions in the peritoneal mesothelium of the mouse embryo. The peritoneal mesothelium of mouse embryos (12 to 18 day of gestation) was studied by freeze-fracture and in sections in order to reveal the initial formation of the tight junctions. Freeze-fracture observations showed three types of tight junctions. Type I consists of belt-like meshworks of elevations on the P face and of shallow grooves on the E face. No tight junctional particle can be seen either on the elevations or in the grooves. Type II shows rows of discontinuous particles on the elevations on the P face. Type III consists of strands forming ridges on the P face. On the E face, the grooves of Type II and III appear to be narrower and sharper than those of Type I. Quantitatively, Type I junctions are most numerous during the early stages (day 12-13) of embryonic development, while Type III junctions become more common in the later stages, and are the only type seen by day 18. Observations on sections, however, fail to distinguish between the three types. The results suggest that an initial sign of tight junction formation is close apposition of the two cell membranes in the junctional domain, without tight junctional particles. Later, the particles appear to be incorporated in the tight junctions and the strands form by fusion of the particles."}
{"id": "PMID:466672", "title": "A study of gonadal organogenesis, and the factors influencing regeneration following surgical castration in Deroceras reticulatum (Pulmonata:Limacidae).", "content": "At hatching, the hermaphrodite duct of Deroceras reticulatum consists of a single cell type designated the Gonadal Stem Cell (GSC). Proliferation of the GSC leads to the formation of numerous ductules each of which forms one of the acini of the gonad. The germinal and supporting cells are derived entirely from the GSC. The germ cells differentiate first, followed by the Sertoli and follicle cells. At the early sperm stage of gonadal development the hermaphrodite duct differentiates to function as a seminal vesicle. Once the GSC are committed to this change they lose their regenerative ability. The only remaining GSC are the cells of the acinar epithelium, and these retain their germinal potential until the death of the animal. Regeneration will occur from the hermaphrodite duct provided it is in the immature state, i.e., composed of GSC, and is exposed to the hormonal conditions of a young animal. Nervous connections and the presence of an artery are not necessary for this regeneration. The presence of a functional gonad does not inhibit regeneration.", "contents": "A study of gonadal organogenesis, and the factors influencing regeneration following surgical castration in Deroceras reticulatum (Pulmonata:Limacidae). At hatching, the hermaphrodite duct of Deroceras reticulatum consists of a single cell type designated the Gonadal Stem Cell (GSC). Proliferation of the GSC leads to the formation of numerous ductules each of which forms one of the acini of the gonad. The germinal and supporting cells are derived entirely from the GSC. The germ cells differentiate first, followed by the Sertoli and follicle cells. At the early sperm stage of gonadal development the hermaphrodite duct differentiates to function as a seminal vesicle. Once the GSC are committed to this change they lose their regenerative ability. The only remaining GSC are the cells of the acinar epithelium, and these retain their germinal potential until the death of the animal. Regeneration will occur from the hermaphrodite duct provided it is in the immature state, i.e., composed of GSC, and is exposed to the hormonal conditions of a young animal. Nervous connections and the presence of an artery are not necessary for this regeneration. The presence of a functional gonad does not inhibit regeneration."}
{"id": "PMID:466671", "title": "The air-blood barrier in the human lung. A freeze-fracture study.", "content": "The air-blood barrier was studied in replicas of freeze-fractured lung biopsies collected from healthy human subjects. Adjacent pneumocytes display a belt-like network composed of 3-7 superimposed ridges (\"fibrils\") on the P face and complementary grooves on the E face, i.e., a structure corresponding to a \"tight\" junction. On the other hand, adjacent capillary endothelial cells show a continuous system of 2-4 membrane foldings. These appear mainly as smooth surfaced crests on the P face; on the E face furrows are seen, at the bottom of which a row of particles is situated. This arrangement suggests a \"leaky\" type of junction. Discontinuous occluding junctions are located in the pericytic venular segment of the alveolar vessels. The present findings are in agreement with previous physiological and ultrastructural tracer studies locating the main part of the diffusion barrier for small polar solutes and proteins in the alveolar epithelium. Communicating junctions are demonstrated between type I and type II pneumocytes, indicating intercellular cooperation between these cells of common embryonic origin, but which fulfill different functions in the adult. In the endothelium of the non-muscular alveolar vessels communicating junctions are lacking. Desmosomes occur in the epithelium between type I and type II pneumocytes; square arrays of particles characterize the plasma membrane of type I pneumocytes.", "contents": "The air-blood barrier in the human lung. A freeze-fracture study. The air-blood barrier was studied in replicas of freeze-fractured lung biopsies collected from healthy human subjects. Adjacent pneumocytes display a belt-like network composed of 3-7 superimposed ridges (\"fibrils\") on the P face and complementary grooves on the E face, i.e., a structure corresponding to a \"tight\" junction. On the other hand, adjacent capillary endothelial cells show a continuous system of 2-4 membrane foldings. These appear mainly as smooth surfaced crests on the P face; on the E face furrows are seen, at the bottom of which a row of particles is situated. This arrangement suggests a \"leaky\" type of junction. Discontinuous occluding junctions are located in the pericytic venular segment of the alveolar vessels. The present findings are in agreement with previous physiological and ultrastructural tracer studies locating the main part of the diffusion barrier for small polar solutes and proteins in the alveolar epithelium. Communicating junctions are demonstrated between type I and type II pneumocytes, indicating intercellular cooperation between these cells of common embryonic origin, but which fulfill different functions in the adult. In the endothelium of the non-muscular alveolar vessels communicating junctions are lacking. Desmosomes occur in the epithelium between type I and type II pneumocytes; square arrays of particles characterize the plasma membrane of type I pneumocytes."}
{"id": "PMID:466673", "title": "Observations of the pineal region of non-eutherian mammals.", "content": "A survey has been made of the pineal region of the brain of 11 species of marsupials belonging to 5 families and a species from both families of monotremes. The results show that the pineal body of non-eutherian mammals, although well-defined in all species, has a very varied morphology. Three types of pineal recess occur: (i) a pineal recess in sensu stricto, (ii) an intercommissural pineal recess, and (iii) an infrapineal recess. The existence of nerve fibres which pass through the pineal body and form a spatial link between the habenular and posterior commissures, has been demonstrated in marsupials and monotremes. It is also likely that these animals as well as eutherian mammals possess a nervus conarii. Nerve cells are not a constant feature of the non-eutherian pineal body. The subcommissural organ (SCO) is present in all species. It does not exhibit the same degree of morphological variation as the pineal body. Horizontal sections available for 4 species within 3 families of marsupials show it to be composed of a median portion jointed to bilateral protuberances. Large nerve cells occur within the SCO in all marsupial species; they are absent from the monotreme SCO. Tentatively, the relationship of these neurons to the SCO is considered to be merely one of association. The importance of an extended comparative study of this region in non-eutherian mammals in order to add insight into its phylogeny and function is emphasized.", "contents": "Observations of the pineal region of non-eutherian mammals. A survey has been made of the pineal region of the brain of 11 species of marsupials belonging to 5 families and a species from both families of monotremes. The results show that the pineal body of non-eutherian mammals, although well-defined in all species, has a very varied morphology. Three types of pineal recess occur: (i) a pineal recess in sensu stricto, (ii) an intercommissural pineal recess, and (iii) an infrapineal recess. The existence of nerve fibres which pass through the pineal body and form a spatial link between the habenular and posterior commissures, has been demonstrated in marsupials and monotremes. It is also likely that these animals as well as eutherian mammals possess a nervus conarii. Nerve cells are not a constant feature of the non-eutherian pineal body. The subcommissural organ (SCO) is present in all species. It does not exhibit the same degree of morphological variation as the pineal body. Horizontal sections available for 4 species within 3 families of marsupials show it to be composed of a median portion jointed to bilateral protuberances. Large nerve cells occur within the SCO in all marsupial species; they are absent from the monotreme SCO. Tentatively, the relationship of these neurons to the SCO is considered to be merely one of association. The importance of an extended comparative study of this region in non-eutherian mammals in order to add insight into its phylogeny and function is emphasized."}
{"id": "PMID:466674", "title": "Quantitation of gastrin and somatostatin cell populations in the antral mucosa of the rat. Comparative distribution and evolution through different life stages.", "content": "Total antral gastrin and somatostatin cell populations as well as their relative distribution pattern throughout the antrum were studied in rats with advancing age from birth time to old age. Both endocrine cell populations were estimated, after staining by immunoperoxidase technique, with a quantitative method using serial parallel strips from entire stomachs. Gastrin cells were regularly found at less than 1 h of post-natal life, but were few in number (447 +/- 82) cells. Somatostatin cells, not seen at birth, were observed in all rats at seven post-natal days; then they increased in number less rapidly but more regularly than gastrin cells. During the normal adult period, corrected gastrin cell population corresponds to about 330,000-500,000 cells and corrected somatostatin cell populations to about 130,000-200,000 cells. For the whole antrum the ratio of gastrin cell to somatostatin cell populations decreases through the rat life from 6.5 at 7 days to 1.5 in old age with a stable value, 2.5, during adult period. Examination of the topographical distribution throughout the antrum of these two populations shows that, strip per strip, their numerical ratio varies. Homogeneous values for the latter occur in the middle part of antrum and, as rule, in each group they reflect the mean value calculated for the whole of the antrum.", "contents": "Quantitation of gastrin and somatostatin cell populations in the antral mucosa of the rat. Comparative distribution and evolution through different life stages. Total antral gastrin and somatostatin cell populations as well as their relative distribution pattern throughout the antrum were studied in rats with advancing age from birth time to old age. Both endocrine cell populations were estimated, after staining by immunoperoxidase technique, with a quantitative method using serial parallel strips from entire stomachs. Gastrin cells were regularly found at less than 1 h of post-natal life, but were few in number (447 +/- 82) cells. Somatostatin cells, not seen at birth, were observed in all rats at seven post-natal days; then they increased in number less rapidly but more regularly than gastrin cells. During the normal adult period, corrected gastrin cell population corresponds to about 330,000-500,000 cells and corrected somatostatin cell populations to about 130,000-200,000 cells. For the whole antrum the ratio of gastrin cell to somatostatin cell populations decreases through the rat life from 6.5 at 7 days to 1.5 in old age with a stable value, 2.5, during adult period. Examination of the topographical distribution throughout the antrum of these two populations shows that, strip per strip, their numerical ratio varies. Homogeneous values for the latter occur in the middle part of antrum and, as rule, in each group they reflect the mean value calculated for the whole of the antrum."}
{"id": "PMID:466675", "title": "A comparison of receptive and non-receptive plasma membrane areas of photoreceptor cells in the leech, Hirudo medicinalis.", "content": "Microvillar (receptive) and external (non-receptive) portions of the plasmalemma of photoreceptor cells of Hirudo were compared electron microscopically in thin sections and freeze-fracture replicas. A morphometric approximation showed that the surface area of the microvillar membrane is about 19 times larger than that of the external membrane. The microvillar membrane most probably undergoes extensive membrane turnover. In both segments of the membrane the particles associated with the P- and the E-fracture faces are randomly distributed except at some specific sites. The particles adhere predominantly to the P-faces. The particle densities on the fracture faces of the microvillar membrane differ from those of the external membrane. The P-face particles of the external membrane appear to be larger than those of the microvillar membrane. It is suggested that the P-face particles of the microvillar membrane represent sites where the photopigment is incorporated into the membrane. The distinguishing structural features correspond to the functional differences postulated for both portions of the plasma membrane.", "contents": "A comparison of receptive and non-receptive plasma membrane areas of photoreceptor cells in the leech, Hirudo medicinalis. Microvillar (receptive) and external (non-receptive) portions of the plasmalemma of photoreceptor cells of Hirudo were compared electron microscopically in thin sections and freeze-fracture replicas. A morphometric approximation showed that the surface area of the microvillar membrane is about 19 times larger than that of the external membrane. The microvillar membrane most probably undergoes extensive membrane turnover. In both segments of the membrane the particles associated with the P- and the E-fracture faces are randomly distributed except at some specific sites. The particles adhere predominantly to the P-faces. The particle densities on the fracture faces of the microvillar membrane differ from those of the external membrane. The P-face particles of the external membrane appear to be larger than those of the microvillar membrane. It is suggested that the P-face particles of the microvillar membrane represent sites where the photopigment is incorporated into the membrane. The distinguishing structural features correspond to the functional differences postulated for both portions of the plasma membrane."}
{"id": "PMID:466677", "title": "A new type of synapse in the ventral cochlear nucleus.", "content": "In this paper we report the appearance of flat vesicle-containing endings in aldehyde-fixed ventral cochlear nucleus of rats with qualitative and quantitative properties suggesting they should be identified as calyceal processes. Their synaptic vesicles are elongate and significantly smaller than the vesicles in the calyces of Lenn and Reese (1966). Therefore these endings are flat vesicular calyceal processes, possibly of inhibitory function.", "contents": "A new type of synapse in the ventral cochlear nucleus. In this paper we report the appearance of flat vesicle-containing endings in aldehyde-fixed ventral cochlear nucleus of rats with qualitative and quantitative properties suggesting they should be identified as calyceal processes. Their synaptic vesicles are elongate and significantly smaller than the vesicles in the calyces of Lenn and Reese (1966). Therefore these endings are flat vesicular calyceal processes, possibly of inhibitory function."}
{"id": "PMID:466678", "title": "Control of the pars intermedia of the lizard, Anolis carolinensis. II. Ultrastructure of the intact intermediate lobe.", "content": "The ultrastructure of the intermediate lobe of the hypophysis was studied in Anolis carolinensis with the use of a threefold aldehyde fixative. Lizards with a brown skin were selected. The possibility of two types of secretory cells is discussed; neither cell type is innervated. Type I cells are rarely found and contain dense granules approximately 0.3 micron in diameter; Type II cells vary widely in secretory activity. Most of the Type II cells contain a large number of dense secretory granules (up to about 1.3 micron in diameter) almost filling the cytoplasm. Rough endoplasmic reticulum (RER), Golgi apparatus and mitochondria are poorly developed. Only some of these cells show signs suggesting a high secretory activity, namely a well developed RER, Golgi apparatus and numerous mitochondria. In these cells the RER sometimes forms large intracisternal droplets (up to 7 micron in diameter). Two of the animals exhibited a more uniform, high secretory activity. Large (about 2 micron in diameter), pale vacuoles, probably of extracellular character, were found mostly in the vicinity of the perivascular septum. Their role in the release of MSH is discussed. The present data, which are discussed with reference to earlier findings (Forbes, 1972), form the morphological basis for an experimental study on regulation of MSH release (Larsson et al., 1979).", "contents": "Control of the pars intermedia of the lizard, Anolis carolinensis. II. Ultrastructure of the intact intermediate lobe. The ultrastructure of the intermediate lobe of the hypophysis was studied in Anolis carolinensis with the use of a threefold aldehyde fixative. Lizards with a brown skin were selected. The possibility of two types of secretory cells is discussed; neither cell type is innervated. Type I cells are rarely found and contain dense granules approximately 0.3 micron in diameter; Type II cells vary widely in secretory activity. Most of the Type II cells contain a large number of dense secretory granules (up to about 1.3 micron in diameter) almost filling the cytoplasm. Rough endoplasmic reticulum (RER), Golgi apparatus and mitochondria are poorly developed. Only some of these cells show signs suggesting a high secretory activity, namely a well developed RER, Golgi apparatus and numerous mitochondria. In these cells the RER sometimes forms large intracisternal droplets (up to 7 micron in diameter). Two of the animals exhibited a more uniform, high secretory activity. Large (about 2 micron in diameter), pale vacuoles, probably of extracellular character, were found mostly in the vicinity of the perivascular septum. Their role in the release of MSH is discussed. The present data, which are discussed with reference to earlier findings (Forbes, 1972), form the morphological basis for an experimental study on regulation of MSH release (Larsson et al., 1979)."}
{"id": "PMID:466679", "title": "Synaptogenic effect of estrogen on the hypothalamic arcuate nucleus of the adult female rat.", "content": "In order to examine the effect of estrogen on the synaptic structures in the hypothalamic arcuate nucleus (ARCN), semi-quantitative studies were performed by counting synapses in an 18,000 micron 2 area in the middle part of the ARCN in each brain. In ovariectomized female rats injected with 2 micrograms of estradiol benzoate (EB) for three weeks, the mean numbers of axodendritic and axosomatic synapses were not significantly different from those in the intact and ovariectomized controls. When the medial basal hypothalamus (MBH) including the ARCN was isolated by use of a Hal\u00e1sz knife (MBH island), the mean number of axodendritic synapses was decreased to about half of the controls. However, EB treatment for three weeks from the day of surgery effectively restored the axodendritic synaptic population of the deafferented ARCN. This may suggest that estrogen has a facilitatory effect on axodendritic synapse formation in the deafferented ARCN, presumably by stimulating axonal sprouting and synaptic regeneration of intact axons in the MBH island.", "contents": "Synaptogenic effect of estrogen on the hypothalamic arcuate nucleus of the adult female rat. In order to examine the effect of estrogen on the synaptic structures in the hypothalamic arcuate nucleus (ARCN), semi-quantitative studies were performed by counting synapses in an 18,000 micron 2 area in the middle part of the ARCN in each brain. In ovariectomized female rats injected with 2 micrograms of estradiol benzoate (EB) for three weeks, the mean numbers of axodendritic and axosomatic synapses were not significantly different from those in the intact and ovariectomized controls. When the medial basal hypothalamus (MBH) including the ARCN was isolated by use of a Hal\u00e1sz knife (MBH island), the mean number of axodendritic synapses was decreased to about half of the controls. However, EB treatment for three weeks from the day of surgery effectively restored the axodendritic synaptic population of the deafferented ARCN. This may suggest that estrogen has a facilitatory effect on axodendritic synapse formation in the deafferented ARCN, presumably by stimulating axonal sprouting and synaptic regeneration of intact axons in the MBH island."}
{"id": "PMID:466680", "title": "Estrogen target cells in the pituitary of platyfish, Xiphophorus maculatus.", "content": "The distribution of estradiol-concentrating cells in the pituitary of the platyfish, Xiphophorus maculatus, is studied after the injection of 3H estradiol-17 beta by thaw-mount autoradiography. Autoradiograms prepared 2-8 h after the injection show nuclear concentration of radioactivity in certain cells of the rostral pars distalis, pars intermedia and pars nervosa. Radioactively labeled cells are identified as gonadotropes by a combined technique of thaw-mount autoradiography and immunoperoxidase staining with antiserum to ovine LH. Approximately 80% of the immunoreactive LH cells show a concentration of radioactivity in their nuclei. These observations indicate that in teleosts, as in mammals, estradiol has a direct effect on pituitary gonadotropes.", "contents": "Estrogen target cells in the pituitary of platyfish, Xiphophorus maculatus. The distribution of estradiol-concentrating cells in the pituitary of the platyfish, Xiphophorus maculatus, is studied after the injection of 3H estradiol-17 beta by thaw-mount autoradiography. Autoradiograms prepared 2-8 h after the injection show nuclear concentration of radioactivity in certain cells of the rostral pars distalis, pars intermedia and pars nervosa. Radioactively labeled cells are identified as gonadotropes by a combined technique of thaw-mount autoradiography and immunoperoxidase staining with antiserum to ovine LH. Approximately 80% of the immunoreactive LH cells show a concentration of radioactivity in their nuclei. These observations indicate that in teleosts, as in mammals, estradiol has a direct effect on pituitary gonadotropes."}
{"id": "PMID:466681", "title": "Fine structure of the transitional zone of the rat seminiferous tubule.", "content": "An electron microscopic study was made on the structure of the testicular transitional zone (TZ) in the adult rat. The TZ proper consists of modified Sertoli cellss, with only a few spermatogonia and macrophages, surrounding distally a very narrow lumen. The TZ Sertoli cells have nuclei with a somewhat coarser matrix and more peripheral heterochromatin than Sertoli cell nuclei of the nearby seminiferous tubules, and the electron density of the cytoplasm varies from cell to cell. Smooth endoplasmic reticulum is abundant, but usually there are also scattered ribosomal rosettes and an occasional profile of rough endoplasmic reticulum. Microtubules are very numerous in the columnar portion of the cell, and laminar structures seemingly joining the cell surfaces are sometimes seen. Lipid droplets and lysosmal structures are frequent cellular components in proximal TZ Sertoli cells. Empty intracellular vacuoles are abundant, sometimes arranged around areas of smooth endoplasmic reticulum. Occasionally, membrane-limited fine granules and vacuoles are seen within Sertoli cells and also in the TZ lumen, suggesting a possible secretory activity by these cells. The apical processes of the Sertoli cells form large vacuolar structures, and in the basal parts of the epithelium vacuoles with capillary-like appearance are frequently seen. Phagocytosis of germinal cells by the Sertoli cells occurs in the proximal region of the TZ. Round waste bodies in contact with the Sertoli cell apices protruding into the tubulus rectus, are also common. The tunica propria of the TZ is thickened and somewhat wrinkled, and in the proximal region the myoid cell layer loses its continuity and is replaced by fibroblasts. The epithelium of the tubulus rectus adjacent to the TZ consists of several overlapping epithelial cells. The typical junctional complexes between TZ Sertoli cells appear to be impermeable to the lanthanum tracer.", "contents": "Fine structure of the transitional zone of the rat seminiferous tubule. An electron microscopic study was made on the structure of the testicular transitional zone (TZ) in the adult rat. The TZ proper consists of modified Sertoli cellss, with only a few spermatogonia and macrophages, surrounding distally a very narrow lumen. The TZ Sertoli cells have nuclei with a somewhat coarser matrix and more peripheral heterochromatin than Sertoli cell nuclei of the nearby seminiferous tubules, and the electron density of the cytoplasm varies from cell to cell. Smooth endoplasmic reticulum is abundant, but usually there are also scattered ribosomal rosettes and an occasional profile of rough endoplasmic reticulum. Microtubules are very numerous in the columnar portion of the cell, and laminar structures seemingly joining the cell surfaces are sometimes seen. Lipid droplets and lysosmal structures are frequent cellular components in proximal TZ Sertoli cells. Empty intracellular vacuoles are abundant, sometimes arranged around areas of smooth endoplasmic reticulum. Occasionally, membrane-limited fine granules and vacuoles are seen within Sertoli cells and also in the TZ lumen, suggesting a possible secretory activity by these cells. The apical processes of the Sertoli cells form large vacuolar structures, and in the basal parts of the epithelium vacuoles with capillary-like appearance are frequently seen. Phagocytosis of germinal cells by the Sertoli cells occurs in the proximal region of the TZ. Round waste bodies in contact with the Sertoli cell apices protruding into the tubulus rectus, are also common. The tunica propria of the TZ is thickened and somewhat wrinkled, and in the proximal region the myoid cell layer loses its continuity and is replaced by fibroblasts. The epithelium of the tubulus rectus adjacent to the TZ consists of several overlapping epithelial cells. The typical junctional complexes between TZ Sertoli cells appear to be impermeable to the lanthanum tracer."}
{"id": "PMID:466683", "title": "Embryonic development and secretory differentiation in the pars tuberalis of the mouse hypophysis.", "content": "The pars tuberalis (PT) of the mouse, like that of other mammals, consists mainly of glandular cells rich in glycogen and peculiar to this lobe. In the mouse, the glandular cells are characterized by large, dense secretory vesicles (up to 300 nm in diameter), the abundance of which indicates a marked secretory activity. The PT develops from a distinct antero-ventral area of Rathke's pouch. The border between the anlagen of the PT and the pars distalis is formed by Atwell's recessus which represents the access for the vessels afferent to the pars distalis. The pedicle of Rathke's pouch is incorporated into the PT anlage, thus contributing to its formation. The entire PT anlage is characterized by glycogen accumulation from the commencement of its formation and persisting in the adult tuberal lobe. Secretory differentiation of the glandular cells of the PT occurs at day 12 of gestation, preceding that of all other adenohypophysial cell types. The secretory features of these cells (development of ergastoplasm and Golgi apparatus, abundance of dense secretory vesicles) appear at an early stage of the enbryonic life (14 days) comparable to those of mature cells. These results confirm earlier observations in the foetal rat where hypophysial secretion also begins in the PT. The existence of peculiar glandular cells speaks in favour of a specific but still unknown function of the PT during foetal and adult life.", "contents": "Embryonic development and secretory differentiation in the pars tuberalis of the mouse hypophysis. The pars tuberalis (PT) of the mouse, like that of other mammals, consists mainly of glandular cells rich in glycogen and peculiar to this lobe. In the mouse, the glandular cells are characterized by large, dense secretory vesicles (up to 300 nm in diameter), the abundance of which indicates a marked secretory activity. The PT develops from a distinct antero-ventral area of Rathke's pouch. The border between the anlagen of the PT and the pars distalis is formed by Atwell's recessus which represents the access for the vessels afferent to the pars distalis. The pedicle of Rathke's pouch is incorporated into the PT anlage, thus contributing to its formation. The entire PT anlage is characterized by glycogen accumulation from the commencement of its formation and persisting in the adult tuberal lobe. Secretory differentiation of the glandular cells of the PT occurs at day 12 of gestation, preceding that of all other adenohypophysial cell types. The secretory features of these cells (development of ergastoplasm and Golgi apparatus, abundance of dense secretory vesicles) appear at an early stage of the enbryonic life (14 days) comparable to those of mature cells. These results confirm earlier observations in the foetal rat where hypophysial secretion also begins in the PT. The existence of peculiar glandular cells speaks in favour of a specific but still unknown function of the PT during foetal and adult life."}
{"id": "PMID:466684", "title": "Formation of sarcomeres in the embryonic heart of the lobster.", "content": "Cardiomyoblasts in the myocardium of embryonic lobsters at 3-4 weeks and 6 months of development were examined with the transmission electron microscope in order to describe the events in the formation of sarcomeres in a neurogenic cardiac system. Thick and thin myofilaments appear first in the cell periphery near the sarcolemma. They align in parallel in a sequential fashion to form consecutive sarcomeric units. Well-defined A and I bands appear before any semblance of a Z line is present. The initial sarcomere is anchored to the sarcolemma by the insertion of thin myofilaments into a region of electron dense material associated intimately with the sarcolemma. Myofibrils grow outward in several planes away from the electron-dense regions of membrane that serve as focal points for myofibril formation.", "contents": "Formation of sarcomeres in the embryonic heart of the lobster. Cardiomyoblasts in the myocardium of embryonic lobsters at 3-4 weeks and 6 months of development were examined with the transmission electron microscope in order to describe the events in the formation of sarcomeres in a neurogenic cardiac system. Thick and thin myofilaments appear first in the cell periphery near the sarcolemma. They align in parallel in a sequential fashion to form consecutive sarcomeric units. Well-defined A and I bands appear before any semblance of a Z line is present. The initial sarcomere is anchored to the sarcolemma by the insertion of thin myofilaments into a region of electron dense material associated intimately with the sarcolemma. Myofibrils grow outward in several planes away from the electron-dense regions of membrane that serve as focal points for myofibril formation."}
{"id": "PMID:466685", "title": "Vascular organization of the catfish gill filament.", "content": "Light and scanning electron microscopic observations were made on methyl-methacrylate corrosion casts of the blood vessels in the gills of channel catfish (Ictalurus punctatus). The vasculature of the gill filament can be divided into three distinct pathways: 1. the well-known respiratory circulation which includes the afferent filamental artery (AF), afferent lamellar arteriole (AL), lamella (L), efferent lamellar arteriole (EL) and efferent filamental artery (EF), 2. a nutritive pathway from the EF through small nutritive capillaries (NC) and into one of several filamental veins (FV), and 3. an interlamellar circulation in which small prelamellar arterio-venous anastomoses (PAVA) connect the AL into a series of organized vascular spaces (interlamellar vessels, ILV's) that underlie the interlamellar filamental epithelium. Several sinus like spaces associated with AF, EF and the filamental cartilagenous support were also observed. The physiological significance of these vascular pathways is discussed.", "contents": "Vascular organization of the catfish gill filament. Light and scanning electron microscopic observations were made on methyl-methacrylate corrosion casts of the blood vessels in the gills of channel catfish (Ictalurus punctatus). The vasculature of the gill filament can be divided into three distinct pathways: 1. the well-known respiratory circulation which includes the afferent filamental artery (AF), afferent lamellar arteriole (AL), lamella (L), efferent lamellar arteriole (EL) and efferent filamental artery (EF), 2. a nutritive pathway from the EF through small nutritive capillaries (NC) and into one of several filamental veins (FV), and 3. an interlamellar circulation in which small prelamellar arterio-venous anastomoses (PAVA) connect the AL into a series of organized vascular spaces (interlamellar vessels, ILV's) that underlie the interlamellar filamental epithelium. Several sinus like spaces associated with AF, EF and the filamental cartilagenous support were also observed. The physiological significance of these vascular pathways is discussed."}
{"id": "PMID:466686", "title": "Membrane specializations in the peripheral retina of the housefly Musca domestica L.", "content": "Membrane specializations of the peripheral retina of the housefly (Musca domestica) are revealed in thin sections and freeze fracture/etch replicas. Septate junctions are abundant in corner areas of the pseudocone enclosure bonding: between homologous corneal pigment cells (CPC); between homologous large pigment cells (LPC); between CPC-LPC; between Semper cells (SC); between SC-CPC. Spot desmosomes are present between Semper cells. It is likely that septate junctions function as strengthening adhesions in this area. A new membrane specialization similar to a continuous junction was observed between retinular cells of the same or adjacent ommatidium. This junction has indistinct septa in the 115 A intermembrane cleft and is intermittent in character. When this junction is absent, the apposed cells gape apart. In freeze fracture studies, this junction is characterized by bridges composed of fused membrane particles and randomly arranged particles on the P face, and noncorresponding grooves on the E face. The ridges are elongate and roughly parallel and sometimes they form enclosures. Mitochondria line up along these junctions, often within 90 A of the unit membrane. This membrane specialization has characteristics of tight and continuous junctions. In line with previous findings, we suggest that this junction assists in retinular cell orientation, possibly in enforcing the ommatidial twist and in maintaining localized ionic concentration gradients between retinular cells.", "contents": "Membrane specializations in the peripheral retina of the housefly Musca domestica L. Membrane specializations of the peripheral retina of the housefly (Musca domestica) are revealed in thin sections and freeze fracture/etch replicas. Septate junctions are abundant in corner areas of the pseudocone enclosure bonding: between homologous corneal pigment cells (CPC); between homologous large pigment cells (LPC); between CPC-LPC; between Semper cells (SC); between SC-CPC. Spot desmosomes are present between Semper cells. It is likely that septate junctions function as strengthening adhesions in this area. A new membrane specialization similar to a continuous junction was observed between retinular cells of the same or adjacent ommatidium. This junction has indistinct septa in the 115 A intermembrane cleft and is intermittent in character. When this junction is absent, the apposed cells gape apart. In freeze fracture studies, this junction is characterized by bridges composed of fused membrane particles and randomly arranged particles on the P face, and noncorresponding grooves on the E face. The ridges are elongate and roughly parallel and sometimes they form enclosures. Mitochondria line up along these junctions, often within 90 A of the unit membrane. This membrane specialization has characteristics of tight and continuous junctions. In line with previous findings, we suggest that this junction assists in retinular cell orientation, possibly in enforcing the ommatidial twist and in maintaining localized ionic concentration gradients between retinular cells."}
{"id": "PMID:466687", "title": "The effect of L-DOPA on the ultrastructure of the adenohypophysis of the Chinese quail, Excalfactoria chinensis.", "content": "Intraperitoneally injected L-DOPA was found to stimulate strongly the lysosomal system of all of the cell types of the adenohypophysis. We suggest that this amine may play a role in the regulation of the secretory activity of the gland by mediating the lysosomal activity, this role being of variable importance according to the fluctuations in hormonal activity. Utrastructural changes occur in some cell types following injection of L-DOPA. The question as to whether these changes are induced directly by L-DOPA or via the monoaminergic system of the hypothalamus and its hypophysiotropic hormones is discussed.", "contents": "The effect of L-DOPA on the ultrastructure of the adenohypophysis of the Chinese quail, Excalfactoria chinensis. Intraperitoneally injected L-DOPA was found to stimulate strongly the lysosomal system of all of the cell types of the adenohypophysis. We suggest that this amine may play a role in the regulation of the secretory activity of the gland by mediating the lysosomal activity, this role being of variable importance according to the fluctuations in hormonal activity. Utrastructural changes occur in some cell types following injection of L-DOPA. The question as to whether these changes are induced directly by L-DOPA or via the monoaminergic system of the hypothalamus and its hypophysiotropic hormones is discussed."}
{"id": "PMID:466688", "title": "Development of the basal lamina and extracellular materials in the early chick embryo.", "content": "Chick embryos at developmental stages up to primitive streak formation were fixed in a mixture of tannic acid and glutaraldehyde. A basal lamina was present in the unincubated embryo and consisted of a lucent lamina interna and a lamina densa. At the primitive streak stage the lamina densa showed a periodicity of stained elements. Densely stained materials were present on the cell surfaces lining the cavity between the epiblast and endoblast, and on the mesoderm cells within this cavity. Considerable amounts of extracellular material were observed in the cavity. Hyaluronidase treatment removed the cell surface and extracellular material, indicating that hyaluronic acid is a major component. This enzyme disrupted the basal lamina, leaving a fibrillar remnant with  no periodic structure. It is therefore suggested that the dense periodicities consist of glycosaminoglycan built on an enzyme-resistant framework which is probably collagen. Enzyme-resistant fibrils, presumably collagen precursors, are present elsewhere within the tissue spaces.", "contents": "Development of the basal lamina and extracellular materials in the early chick embryo. Chick embryos at developmental stages up to primitive streak formation were fixed in a mixture of tannic acid and glutaraldehyde. A basal lamina was present in the unincubated embryo and consisted of a lucent lamina interna and a lamina densa. At the primitive streak stage the lamina densa showed a periodicity of stained elements. Densely stained materials were present on the cell surfaces lining the cavity between the epiblast and endoblast, and on the mesoderm cells within this cavity. Considerable amounts of extracellular material were observed in the cavity. Hyaluronidase treatment removed the cell surface and extracellular material, indicating that hyaluronic acid is a major component. This enzyme disrupted the basal lamina, leaving a fibrillar remnant with  no periodic structure. It is therefore suggested that the dense periodicities consist of glycosaminoglycan built on an enzyme-resistant framework which is probably collagen. Enzyme-resistant fibrils, presumably collagen precursors, are present elsewhere within the tissue spaces."}
{"id": "PMID:466689", "title": "The hypothalamo-hypophysial system in the ground squirrel, Citellus erythrogenys Brandt. II. Seasonal changes in the classical neurosecretory system of a hibernator.", "content": "Monthly observations of the \"Gomori-positive\" hypothalamo-hypophysial neurosecretory system (HHNS) of the ground squirrel, Citellus erythrogenys Brandt, were carried out light microscopically using several quantitative methods. From the beginning of hibernation, formation of neurosecretory material (NSM) in the neurosecretory cells (NSC) progressively decreases and release of neurohormones from the HHNS is almost fully inhibited. A maximal accumulation of NSM in the perikarya of the NSC and in the posterior pituitary (PP) is found in December. By this time the volume of the cell nuclei and nucleoli is at a minimum. Signs of activation of the HHNS appear and become more conspicuous as the time of arousal from torpor approaches. The amount of NSM in the NSC and the PP decreases simultaneously with the increase in volume of the NSC. Hyperemia and activation of glial elements is visible throughout the HHNS. The morphological signs of activation reach their peak in March. After reproduction is completed (April to beginning of May), the NSC and the PP are almost devoid of NSM. Beginning with June and during the summer and autumn months a progressive accumulation of NSM in the NSC and the PP parallels gradual diminution in the volume of the NSC structures and the glial cells. Mechanisms and effector pathways by which the HHNS influences seasonal adaptation of the organism and reproduction are discussed.", "contents": "The hypothalamo-hypophysial system in the ground squirrel, Citellus erythrogenys Brandt. II. Seasonal changes in the classical neurosecretory system of a hibernator. Monthly observations of the \"Gomori-positive\" hypothalamo-hypophysial neurosecretory system (HHNS) of the ground squirrel, Citellus erythrogenys Brandt, were carried out light microscopically using several quantitative methods. From the beginning of hibernation, formation of neurosecretory material (NSM) in the neurosecretory cells (NSC) progressively decreases and release of neurohormones from the HHNS is almost fully inhibited. A maximal accumulation of NSM in the perikarya of the NSC and in the posterior pituitary (PP) is found in December. By this time the volume of the cell nuclei and nucleoli is at a minimum. Signs of activation of the HHNS appear and become more conspicuous as the time of arousal from torpor approaches. The amount of NSM in the NSC and the PP decreases simultaneously with the increase in volume of the NSC. Hyperemia and activation of glial elements is visible throughout the HHNS. The morphological signs of activation reach their peak in March. After reproduction is completed (April to beginning of May), the NSC and the PP are almost devoid of NSM. Beginning with June and during the summer and autumn months a progressive accumulation of NSM in the NSC and the PP parallels gradual diminution in the volume of the NSC structures and the glial cells. Mechanisms and effector pathways by which the HHNS influences seasonal adaptation of the organism and reproduction are discussed."}
{"id": "PMID:466690", "title": "Control of the pars intermedia of the lizard, Anolis carolinensis. III. Changes in the ultrastructure of the disconnected neuro-intermediate lobe.", "content": "Morphological changes in the disconnected neuro-intermediate lobe were studied in the lizard, Anolis carolinensis from the 2nd to the 14th post-operative day using a threefold aldehyde fixative (Rodr\u00edguez, 1969). Two phases of colour change capacity were exhibited: Phase I started immediately after the transection, lasted for 6 days (mean) and was characterised by an excessive MSH release (brown skin). This phase proceeded gradually into Phase II, designated by an interruption by the MSH release (green skin). The degenerative processes and final elimination of neurons in the disconnected neural lobe propagate in a rostro-caudal direction from the transected area. The aminergic fibres (Type II) disappear within 2 days postoperatively, whereas the degeneration continues for more than 10 days in the peptidergic fibres (Type III, IV and V). The glia cells (ependyma and pituicytes) serve as very active macrophages, engulfing fragments of axons already affected by autolysis and transferring them into glial lysosomes. No apparent morphological changes occur in the shift from Phase I to II. The great majority of the secretory cells of the intermediate lobe are not affected by degenerative processes and appear to be markedly activated by the stalk transection. They exhibit numerous mitochondria, well-developed Golgi complexes forming numerous Golgi vesicles and extensive parallel cisternae of the rough endoplasmic reticulum, sometimes forming large intracisternal droplets (7 micron in diameter). Numerous pale vacuoles are seen, especially toward the intact capillaries, suggesting their coupling to the MSH release by extension of the active membrane area toward the perivascular septum. The number of these vacuoles is very markedly reduced in Phase II (no release), whereas the formation of new granules seems to proceed in early stages. The interruption of the MSH release implies a successive refilling of gradually growing secretory granules and a concomitant reduction in the development of the synthetic apparatus. Mechanisms probably involved in the control of the synthesis and release of MSH are discussed.", "contents": "Control of the pars intermedia of the lizard, Anolis carolinensis. III. Changes in the ultrastructure of the disconnected neuro-intermediate lobe. Morphological changes in the disconnected neuro-intermediate lobe were studied in the lizard, Anolis carolinensis from the 2nd to the 14th post-operative day using a threefold aldehyde fixative (Rodr\u00edguez, 1969). Two phases of colour change capacity were exhibited: Phase I started immediately after the transection, lasted for 6 days (mean) and was characterised by an excessive MSH release (brown skin). This phase proceeded gradually into Phase II, designated by an interruption by the MSH release (green skin). The degenerative processes and final elimination of neurons in the disconnected neural lobe propagate in a rostro-caudal direction from the transected area. The aminergic fibres (Type II) disappear within 2 days postoperatively, whereas the degeneration continues for more than 10 days in the peptidergic fibres (Type III, IV and V). The glia cells (ependyma and pituicytes) serve as very active macrophages, engulfing fragments of axons already affected by autolysis and transferring them into glial lysosomes. No apparent morphological changes occur in the shift from Phase I to II. The great majority of the secretory cells of the intermediate lobe are not affected by degenerative processes and appear to be markedly activated by the stalk transection. They exhibit numerous mitochondria, well-developed Golgi complexes forming numerous Golgi vesicles and extensive parallel cisternae of the rough endoplasmic reticulum, sometimes forming large intracisternal droplets (7 micron in diameter). Numerous pale vacuoles are seen, especially toward the intact capillaries, suggesting their coupling to the MSH release by extension of the active membrane area toward the perivascular septum. The number of these vacuoles is very markedly reduced in Phase II (no release), whereas the formation of new granules seems to proceed in early stages. The interruption of the MSH release implies a successive refilling of gradually growing secretory granules and a concomitant reduction in the development of the synthetic apparatus. Mechanisms probably involved in the control of the synthesis and release of MSH are discussed."}
{"id": "PMID:466691", "title": "An ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth Galleria mellonella in vitro.", "content": "The plasmatocytes are the major phagocytic blood-cell type in the haemolymph of the wax-moth, Galleria mellonella. In the present study, these cells were allowed to attach to tissue culture dishes for 1 h, rinsed and then incubated with latex beads for up to 72 h. These cells were then fixed for routine transmission electron microscopy and acid phosphatase cytochemistry. Intracellular latex particles were found in tight, ill-defined phagosomes, which were often clearly associated with the Golgi complexes of the plasmatocytes. Fusion of both primary lysosomes and multivesicular bodies with the phagosomes occasionally occurred and this resulted in the accumulation of an acid phosphatase positive reaction product around the test particles. Subsequent experiments showed that this acid phosphatase activity was mainly associated with the primary lysosomes. The results of the lysosome/latex interactions are compared with those obtained from similar studies on the digestive mechanisms in other phagocytes.", "contents": "An ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth Galleria mellonella in vitro. The plasmatocytes are the major phagocytic blood-cell type in the haemolymph of the wax-moth, Galleria mellonella. In the present study, these cells were allowed to attach to tissue culture dishes for 1 h, rinsed and then incubated with latex beads for up to 72 h. These cells were then fixed for routine transmission electron microscopy and acid phosphatase cytochemistry. Intracellular latex particles were found in tight, ill-defined phagosomes, which were often clearly associated with the Golgi complexes of the plasmatocytes. Fusion of both primary lysosomes and multivesicular bodies with the phagosomes occasionally occurred and this resulted in the accumulation of an acid phosphatase positive reaction product around the test particles. Subsequent experiments showed that this acid phosphatase activity was mainly associated with the primary lysosomes. The results of the lysosome/latex interactions are compared with those obtained from similar studies on the digestive mechanisms in other phagocytes."}
{"id": "PMID:466692", "title": "A cytochemical and immunofluorescence study of endocrine cells in the gut of the ascidian Styela clava.", "content": "Strong secretin-like immunofluorescence has been demonstrated in endocrine-like cells from the gastric epithelium of Styela. These cells also stain with lead haematoxylin and exhibit a brilliant formaldehyde-induced fluorescence, but do not show any other cytochemical features characteristics of the mammalian APUD series. Tests with antisera to glucagon, gastrin and somatostatin all proved negative. In the oesophagus tests with all four antisera proved negative. The significance of these results is discussed in relation to the phylogeny of vertebrate gastro-intestinal hormones.", "contents": "A cytochemical and immunofluorescence study of endocrine cells in the gut of the ascidian Styela clava. Strong secretin-like immunofluorescence has been demonstrated in endocrine-like cells from the gastric epithelium of Styela. These cells also stain with lead haematoxylin and exhibit a brilliant formaldehyde-induced fluorescence, but do not show any other cytochemical features characteristics of the mammalian APUD series. Tests with antisera to glucagon, gastrin and somatostatin all proved negative. In the oesophagus tests with all four antisera proved negative. The significance of these results is discussed in relation to the phylogeny of vertebrate gastro-intestinal hormones."}
{"id": "PMID:466693", "title": "Calcium binding sites in the vesicles of the carotid and aortic body chief cells.", "content": "Chief cells of the carotid and aortic body chemoreceptors possess numerous cytoplasmic dense-core vesicles which are known to contain primarily dopamine. Following fixation in solutions containing 50 mM CaCl2, a 20--30 nm electron-dense particle (EDP) is often observed eccentrically located in many of the vesicles. Approximately 44% of the carotid body and 16% of the aortic body vesicles contain an EDP. The EDP probably represents the Ca++ binding site critical to the stimulus-secretion coupling events culminating in exocytosis of these vesicles. The presence of Ca++ in the cytoplasmic vesicles was verified by electron probe X-ray microanalysis.", "contents": "Calcium binding sites in the vesicles of the carotid and aortic body chief cells. Chief cells of the carotid and aortic body chemoreceptors possess numerous cytoplasmic dense-core vesicles which are known to contain primarily dopamine. Following fixation in solutions containing 50 mM CaCl2, a 20--30 nm electron-dense particle (EDP) is often observed eccentrically located in many of the vesicles. Approximately 44% of the carotid body and 16% of the aortic body vesicles contain an EDP. The EDP probably represents the Ca++ binding site critical to the stimulus-secretion coupling events culminating in exocytosis of these vesicles. The presence of Ca++ in the cytoplasmic vesicles was verified by electron probe X-ray microanalysis."}
{"id": "PMID:466694", "title": "Cell-to-cell contacts in primary cultures of dissociated chicken embryonic brain.", "content": "The fine structure of intercellular contacts was studied in primary cultures prepared from chicken embryonic brain. Desmosomes were frequently seen between the glial cells. Synaptic contacts were observed among neuronal cell bodies and neural processes after 8 days in vitro. Gap junctions were revealed between glial elements suggesting a functional role in direct cell communication and providing a morphological basis for previous observations on potassium transport in cultures of dissociated brain cells.", "contents": "Cell-to-cell contacts in primary cultures of dissociated chicken embryonic brain. The fine structure of intercellular contacts was studied in primary cultures prepared from chicken embryonic brain. Desmosomes were frequently seen between the glial cells. Synaptic contacts were observed among neuronal cell bodies and neural processes after 8 days in vitro. Gap junctions were revealed between glial elements suggesting a functional role in direct cell communication and providing a morphological basis for previous observations on potassium transport in cultures of dissociated brain cells."}
{"id": "PMID:466695", "title": "Neuro-epithelial bodies in organ cultures of fetal rabbit lungs. Ultrastructural characteristics and effects of drugs.", "content": "Lung explants from fetal rabbit at the late glandular stage of development (20 days' gestation) and near term (31 days' gestation) were maintained in organ culture for up to 22 days. They were studied by light and electron microscopy to determine whether neuro-epithelial bodies (NEB) of the lung retain structural integrity in vitro. Cultured NEB retained argyrophilia and specific amine fluorescence after formaldehyde condensation. Their ultrastructural morphology showed some differences from that of uncultured NEB: the terminal axons had degenerated and the secretory granules (dense-core vesicles, DCV) were slightly larger, more pleomorphic, more electron-dense, and redistributed throughout the cytoplasm rather than being confined chiefly to the basal regions. These changes, together with hypertrophy of Golgi zones, suggest increased synthesis and storage of secretory products in the DCV during culture. In NEB from near-term explants cultured for 7 days and incubated with reserpine, the core of DCV decreased in size and electron-density and became finely granular, a sign of amine release. Ca++ ionophore No. A-23187, also, induced changes in the ultrastructure of DCV, suggesting that the secretory process in lung neuro-endocrine cells, as in other secretory cells, is Ca++-dependent.", "contents": "Neuro-epithelial bodies in organ cultures of fetal rabbit lungs. Ultrastructural characteristics and effects of drugs. Lung explants from fetal rabbit at the late glandular stage of development (20 days' gestation) and near term (31 days' gestation) were maintained in organ culture for up to 22 days. They were studied by light and electron microscopy to determine whether neuro-epithelial bodies (NEB) of the lung retain structural integrity in vitro. Cultured NEB retained argyrophilia and specific amine fluorescence after formaldehyde condensation. Their ultrastructural morphology showed some differences from that of uncultured NEB: the terminal axons had degenerated and the secretory granules (dense-core vesicles, DCV) were slightly larger, more pleomorphic, more electron-dense, and redistributed throughout the cytoplasm rather than being confined chiefly to the basal regions. These changes, together with hypertrophy of Golgi zones, suggest increased synthesis and storage of secretory products in the DCV during culture. In NEB from near-term explants cultured for 7 days and incubated with reserpine, the core of DCV decreased in size and electron-density and became finely granular, a sign of amine release. Ca++ ionophore No. A-23187, also, induced changes in the ultrastructure of DCV, suggesting that the secretory process in lung neuro-endocrine cells, as in other secretory cells, is Ca++-dependent."}
{"id": "PMID:466696", "title": "Spatial distribution of excitatory innervation on a muscle fiber of the lobster (Homarus americanus).", "content": "The distribution of nerve terminals from a single excitatory motor axon has been followed along a fiber from the proximal accessory flexor muscle of the lobster by thin serial sectioning at periodic intervals. The excitatory motor axon provides small axonal branches (diameter 5--12 micron) of varying lengths that travel along the surface of the muscle fiber. Each of these branches gives rise to discrete synaptic terminals which are not uniformly distributed. Individual terminals vary in length from 10--60 micron and the majority possess neuromuscular synapses. The greatest distance between synapses is slightly over 1 mm which is well within the length constant (2.6 mm) of this muscle fiber. Thus the spatail distribution of synapses is such as to ensure adequate depolarization along the entire length of the muscle fiber.", "contents": "Spatial distribution of excitatory innervation on a muscle fiber of the lobster (Homarus americanus). The distribution of nerve terminals from a single excitatory motor axon has been followed along a fiber from the proximal accessory flexor muscle of the lobster by thin serial sectioning at periodic intervals. The excitatory motor axon provides small axonal branches (diameter 5--12 micron) of varying lengths that travel along the surface of the muscle fiber. Each of these branches gives rise to discrete synaptic terminals which are not uniformly distributed. Individual terminals vary in length from 10--60 micron and the majority possess neuromuscular synapses. The greatest distance between synapses is slightly over 1 mm which is well within the length constant (2.6 mm) of this muscle fiber. Thus the spatail distribution of synapses is such as to ensure adequate depolarization along the entire length of the muscle fiber."}
{"id": "PMID:466698", "title": "Ultrastructural studies on erythropoiesis in the avian thymus. II. A stereological analysis of the lymphoid and erythroid cells.", "content": "The cortex of enlarging thymic lobes from adult haemorrhaged Quelea quelea were found to be similar to those of wild birds where the thymic enlargement was occurring naturally. A detailed stereological analysis of cells broadly designated as lymphoid, and the construction of models to account for the results, indicates that the enlarging thymic lobe contains both large and small blast cells, a heterogenous group of medium lymphocytes, erythroid cells, and two types of very small lymphocytes. The distinction between early erythroid cells and some lymphocytes, despite this detailed analysis is very difficult, but it is possible in enlarging thymic lobes that up to 42% of the lymphoid cells may have erythroid characteristics.", "contents": "Ultrastructural studies on erythropoiesis in the avian thymus. II. A stereological analysis of the lymphoid and erythroid cells. The cortex of enlarging thymic lobes from adult haemorrhaged Quelea quelea were found to be similar to those of wild birds where the thymic enlargement was occurring naturally. A detailed stereological analysis of cells broadly designated as lymphoid, and the construction of models to account for the results, indicates that the enlarging thymic lobe contains both large and small blast cells, a heterogenous group of medium lymphocytes, erythroid cells, and two types of very small lymphocytes. The distinction between early erythroid cells and some lymphocytes, despite this detailed analysis is very difficult, but it is possible in enlarging thymic lobes that up to 42% of the lymphoid cells may have erythroid characteristics."}
{"id": "PMID:466697", "title": "Ultrastructural studies on erythropoiesis in the avian thymus. I. Description of cell types.", "content": "Thymus lobes from three species of birds, Quelea quelea, Passer domesticus and Sturnus vulgaris, have been examined ultrastructurally. The component cell types are compared with their counterparts in mammalian thymus glands, and found to be similar. Greater differences between small, intermediate and enlarged lobes of one species than exist between species. Developing erythroid cells are present in most enlarging and some enlarged glands. They appear to be developing at the expense of lymphoid cells in some birds. The origin of these cells is discussed. Cells that are possible candidates for the production of some thymic hormones are also described.", "contents": "Ultrastructural studies on erythropoiesis in the avian thymus. I. Description of cell types. Thymus lobes from three species of birds, Quelea quelea, Passer domesticus and Sturnus vulgaris, have been examined ultrastructurally. The component cell types are compared with their counterparts in mammalian thymus glands, and found to be similar. Greater differences between small, intermediate and enlarged lobes of one species than exist between species. Developing erythroid cells are present in most enlarging and some enlarged glands. They appear to be developing at the expense of lymphoid cells in some birds. The origin of these cells is discussed. Cells that are possible candidates for the production of some thymic hormones are also described."}
{"id": "PMID:466699", "title": "Ionic gradients within mantle-collar epithelial cells of the land snail Otala lactea.", "content": "Aestivating Otala lactea have been shown to reduce the rate of evaporative water loss from the cells of the mantle-collar epithelium to a level comparable to that of an insect. X-ray microanalyses of ultrathin frozen sections from aestivating and non-aestivating snails have shown gradients of chloride and potassium ions in the apical microvillus region of the regulating mantle collar epithelium. The greatest difference in osmotic concentration occurs in the apical 2 micron of the cell. There appears to be a barrier at that level that prevents water being mobilised from the underlying tissues. Methods of presenting data generated by X-ray microanalysis are also discussed.", "contents": "Ionic gradients within mantle-collar epithelial cells of the land snail Otala lactea. Aestivating Otala lactea have been shown to reduce the rate of evaporative water loss from the cells of the mantle-collar epithelium to a level comparable to that of an insect. X-ray microanalyses of ultrathin frozen sections from aestivating and non-aestivating snails have shown gradients of chloride and potassium ions in the apical microvillus region of the regulating mantle collar epithelium. The greatest difference in osmotic concentration occurs in the apical 2 micron of the cell. There appears to be a barrier at that level that prevents water being mobilised from the underlying tissues. Methods of presenting data generated by X-ray microanalysis are also discussed."}
{"id": "PMID:466700", "title": "Organization and development of the perivascular space system in the neurohypophysis of the laboratory mouse.", "content": "The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the shallow space around capillary loops of the primary plexus of the portal system, formed radiations into the adjacent neural tissue of the external zone. The tissue of the neural lobe was separable into non-vascular regions dominated by undilated portions of neurosecretory nerve fibres and pituicytes, and neurovascular regions with perivascular space extensions forming an extensive system of connections between neighbouring capillaries. In the median eminence, the system of extensions of the perivascular space was estimated to increase the neurovascular contact surface area by at least 50%, implying an increased efficiency of the organ without a notable increase of its volume. The possibility that the ramifications of the perivascular space imply an enhanced uptake rate into the bloodstream and a subsequent increased concentration of the neurohormones in the portal blood, was discussed. During development of the median eminence, differentiation of perivascular space extensions of the adult type started in the juvenile of about three weeks of age, when shallow capillary loops had been formed. In the neural lobe, perivascular space ramifications were already present when the internal capillaries were formed and were fairly frequent in ten-day young. At the age of three to four weeks the organization of the system was similar to that of the adult animal.", "contents": "Organization and development of the perivascular space system in the neurohypophysis of the laboratory mouse. The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the shallow space around capillary loops of the primary plexus of the portal system, formed radiations into the adjacent neural tissue of the external zone. The tissue of the neural lobe was separable into non-vascular regions dominated by undilated portions of neurosecretory nerve fibres and pituicytes, and neurovascular regions with perivascular space extensions forming an extensive system of connections between neighbouring capillaries. In the median eminence, the system of extensions of the perivascular space was estimated to increase the neurovascular contact surface area by at least 50%, implying an increased efficiency of the organ without a notable increase of its volume. The possibility that the ramifications of the perivascular space imply an enhanced uptake rate into the bloodstream and a subsequent increased concentration of the neurohormones in the portal blood, was discussed. During development of the median eminence, differentiation of perivascular space extensions of the adult type started in the juvenile of about three weeks of age, when shallow capillary loops had been formed. In the neural lobe, perivascular space ramifications were already present when the internal capillaries were formed and were fairly frequent in ten-day young. At the age of three to four weeks the organization of the system was similar to that of the adult animal."}
{"id": "PMID:466705", "title": "The sea urchin (Sphaerechinus granularis) codes different H2B histones to assemble sperm and embryo chromatin.", "content": "Histones from sperm and gastrula nuclei of the sea urchin Sphaerechinus granularis were analyzed by three different electrophoretic methods, stained with specific color reactions and compared with one another. The histones of the two cell types showed identical properties in none of the three analytical methods employed but the greatest differences involved the H2B type of histones. H2B from embryo chromatin had properties similar to those of the corresponding molecule from calf thymus. In the sperm chromatin no such molecule was observed but two additional ones were found that differed from embryo H2B in size, charge and specific colour reactions. The differences were such that different genes may be postulated for the synthesis of the H2B histones found in sperm and in embryo.", "contents": "The sea urchin (Sphaerechinus granularis) codes different H2B histones to assemble sperm and embryo chromatin. Histones from sperm and gastrula nuclei of the sea urchin Sphaerechinus granularis were analyzed by three different electrophoretic methods, stained with specific color reactions and compared with one another. The histones of the two cell types showed identical properties in none of the three analytical methods employed but the greatest differences involved the H2B type of histones. H2B from embryo chromatin had properties similar to those of the corresponding molecule from calf thymus. In the sperm chromatin no such molecule was observed but two additional ones were found that differed from embryo H2B in size, charge and specific colour reactions. The differences were such that different genes may be postulated for the synthesis of the H2B histones found in sperm and in embryo."}
{"id": "PMID:466706", "title": "Metabolic stability of nonhistone chromosomal proteins in two different classes of developing rat brain nuclei.", "content": "The metabolic behaviour of NHCP was studied in rat brain nuclei from fully differetiated cells and in nuclei from still differentiating cells. Eight-day-old rats were injected intracisternally with [14C]thymidine and [3H]tryptophan and the 3H/14C ratio of the chromatin was followed for a period of 33 days. It was found that in the fraction of differentiated cells this ratio remained unchanged, showing the presence of metabolically stable NHCP. At the same time in the fraction containing nondifferentiated cells a substantial part of the NHCP was turned over, which was indicated by a sharp decrease in their 3H/14C ratio with time.", "contents": "Metabolic stability of nonhistone chromosomal proteins in two different classes of developing rat brain nuclei. The metabolic behaviour of NHCP was studied in rat brain nuclei from fully differetiated cells and in nuclei from still differentiating cells. Eight-day-old rats were injected intracisternally with [14C]thymidine and [3H]tryptophan and the 3H/14C ratio of the chromatin was followed for a period of 33 days. It was found that in the fraction of differentiated cells this ratio remained unchanged, showing the presence of metabolically stable NHCP. At the same time in the fraction containing nondifferentiated cells a substantial part of the NHCP was turned over, which was indicated by a sharp decrease in their 3H/14C ratio with time."}
{"id": "PMID:466707", "title": "DNAase hydrolysis of chromatin DNA in intact sea urchin sperm cells. Effect of the ionic strength on the digestion parameters.", "content": "Chromatin DNA in intact functional Sphaerechinus granularis sperm cells has been digested with micrococcal nuclease at three different ionic strength conditions. The results show a highly-flexible chromatin organization similar to that found in sperm heads. The rate of digestion, the limit value of acid-soluble material and the fragmentation pattern show that the sensitivity of nucleosome and internucleosome DNA regions to nuclease hydrolysis depends on a delicate balance of polar and non polar interactions. At low ionic strength, both nucleosome and internucleosome regions are rapidly and completely hydrolysed at the same time and a transient subunit fragment of 120 b.p. average length is formed. At high ionic strength, internucleosome regions are preferentially hydrolysed; there is a limit digest value and a stable subunit fragment of 140 b.p. average length is formed. A supernucleosome organization in the high ionic strength environment of the sperm cells is suggested by the transient preferential formation of heptamers of nucleosome DNA fragments.", "contents": "DNAase hydrolysis of chromatin DNA in intact sea urchin sperm cells. Effect of the ionic strength on the digestion parameters. Chromatin DNA in intact functional Sphaerechinus granularis sperm cells has been digested with micrococcal nuclease at three different ionic strength conditions. The results show a highly-flexible chromatin organization similar to that found in sperm heads. The rate of digestion, the limit value of acid-soluble material and the fragmentation pattern show that the sensitivity of nucleosome and internucleosome DNA regions to nuclease hydrolysis depends on a delicate balance of polar and non polar interactions. At low ionic strength, both nucleosome and internucleosome regions are rapidly and completely hydrolysed at the same time and a transient subunit fragment of 120 b.p. average length is formed. At high ionic strength, internucleosome regions are preferentially hydrolysed; there is a limit digest value and a stable subunit fragment of 140 b.p. average length is formed. A supernucleosome organization in the high ionic strength environment of the sperm cells is suggested by the transient preferential formation of heptamers of nucleosome DNA fragments."}
{"id": "PMID:466708", "title": "Characterization of presumptive histone messenger RNA from a cell line of Aedes aegypti.", "content": "Four presumptive histone messenger RNAs were characterized from a cell line of Aedes aegypti, and their molecular weights were determined by electrophoresis. They were shown to be associated with polysomes during the peak of DNA synthesis, but not when DNA synthesis was inhibited by cytosine arabinoside or when DNA was not being synthesized. These mRNAs are associated with polysomes containing less than 8 ribosomes and having a high ratio of incorporation of lysine to tryptophan into their nascent peptides. The mRNAs released from these polysomes were translated in vitro and histone products were synthesized. Histones were not synthesized when the mRNAs were obtained from large polysomes or from small polysomes during the non-DNA synthetic period.", "contents": "Characterization of presumptive histone messenger RNA from a cell line of Aedes aegypti. Four presumptive histone messenger RNAs were characterized from a cell line of Aedes aegypti, and their molecular weights were determined by electrophoresis. They were shown to be associated with polysomes during the peak of DNA synthesis, but not when DNA synthesis was inhibited by cytosine arabinoside or when DNA was not being synthesized. These mRNAs are associated with polysomes containing less than 8 ribosomes and having a high ratio of incorporation of lysine to tryptophan into their nascent peptides. The mRNAs released from these polysomes were translated in vitro and histone products were synthesized. Histones were not synthesized when the mRNAs were obtained from large polysomes or from small polysomes during the non-DNA synthetic period."}
{"id": "PMID:466709", "title": "Differentiation without mitosis in Dictyostelium discoideum.", "content": "Dictyoselium discoideum Ax-2 amoebae incubated in the presence of the microtubule inhibitor nocodazole, irreversibly lost their ability to multiply. Nocodazole-treated cells remained viable and RNA and protein synthesis continued for at least 48 h. When nocodazole-treated amoebae were allowed to develop on Millipore filters or on agar slides they differentiated with some delay when compared with controls. These results show that mitosis, naturally present during the developmental cycle of Dictyostelium discoideum Ax-2, is not indispensible for differentiation.", "contents": "Differentiation without mitosis in Dictyostelium discoideum. Dictyoselium discoideum Ax-2 amoebae incubated in the presence of the microtubule inhibitor nocodazole, irreversibly lost their ability to multiply. Nocodazole-treated cells remained viable and RNA and protein synthesis continued for at least 48 h. When nocodazole-treated amoebae were allowed to develop on Millipore filters or on agar slides they differentiated with some delay when compared with controls. These results show that mitosis, naturally present during the developmental cycle of Dictyostelium discoideum Ax-2, is not indispensible for differentiation."}
{"id": "PMID:466722", "title": "Ethanol inhibition of vinyl chloride metabolism in isolated rat hepatocytes.", "content": "Isolated rat liver cells convert [14C]vinyl chloride into non-volatile metabolites. The metabolism is not increased by in vivo pretreatment with phenobarbital. It is sensitive to inhibition by ethanol, which at a concentration of 4 mM inhibits vinyl chloride metabolism to 50% in hepatocyte suspensions. The metabolic activity is NADPH-dependent and is localized in the microsomal fraction of the liver. The enzyme is also strongly inhibited by tetrahydrofuran, indicating that it could be identical to an ethanol-inducible cytochrome P-450 described in the literature [1].", "contents": "Ethanol inhibition of vinyl chloride metabolism in isolated rat hepatocytes. Isolated rat liver cells convert [14C]vinyl chloride into non-volatile metabolites. The metabolism is not increased by in vivo pretreatment with phenobarbital. It is sensitive to inhibition by ethanol, which at a concentration of 4 mM inhibits vinyl chloride metabolism to 50% in hepatocyte suspensions. The metabolic activity is NADPH-dependent and is localized in the microsomal fraction of the liver. The enzyme is also strongly inhibited by tetrahydrofuran, indicating that it could be identical to an ethanol-inducible cytochrome P-450 described in the literature [1]."}
{"id": "PMID:466724", "title": "The interaction of cephaloridine with model membrane systems and rat kidney lysosomes.", "content": "The antibiotic cephaloridine has been shown to interact with phospholipid structures, using the techniques of ultraviolet difference spectroscopy, surface pressure measurements and liposome models. The results indicate that this interaction is at least partly hydrophobic in nature and help explain the disruptive effects of high concentrations of cephaloridine on both artificial and natural phospholipid structures (lysosomes). Low concentrations of cephaloridine were shown to inhibit a lysosomal membrane-bound phospholipase 2 and it is suggested that such an inhibition may explain the cephaloridine-induced stabilization of rat-kidney lysosomes.", "contents": "The interaction of cephaloridine with model membrane systems and rat kidney lysosomes. The antibiotic cephaloridine has been shown to interact with phospholipid structures, using the techniques of ultraviolet difference spectroscopy, surface pressure measurements and liposome models. The results indicate that this interaction is at least partly hydrophobic in nature and help explain the disruptive effects of high concentrations of cephaloridine on both artificial and natural phospholipid structures (lysosomes). Low concentrations of cephaloridine were shown to inhibit a lysosomal membrane-bound phospholipase 2 and it is suggested that such an inhibition may explain the cephaloridine-induced stabilization of rat-kidney lysosomes."}
{"id": "PMID:466725", "title": "Mutagenicity and irreversible binding of the hepatocarcinogen, 2,4-diaminotoluene.", "content": "Mutagenicity of 2,4-diaminotoluene (DAT) in the Salmonella mutagenicity assay was increased with liver fractions from phenobarbital (PB) or beta-naphthoflavone (BNF) treated rats. Substitutions of the hydrogens in the methyl group of 2,4-DAT with deuterium resulted in a decrease in mutagenicity. Incubation of rat liver microsomes with tritiated 2,4-DAT in the presence of NADPH led to the formation of irreversibly bound products to microsomal protein. The rates of binding were not increased using microsomes from PB or BNF-treated rats and was not altered by deuterium substitution in the methyl group. Addition of superoxide dismutase, glutathione (GSH) or rat liver supernatant reduced 2,4-DAT irreversible binding, whereas 2,4-DAT mutagenicity was unaffected by superoxide dismutase addition. Injection of tritiated 2,4-DAT 100 mg/kg to rats lead to its irreversible binding to liver protein and ribosomal RNA and to kidney protein in vivo, again protein binding was not increased after prior treatment with PB or BNF. No irreversible interaction of tritiated 2,4-DAT with DNA either in vitro or in vivo could be demonstrated.", "contents": "Mutagenicity and irreversible binding of the hepatocarcinogen, 2,4-diaminotoluene. Mutagenicity of 2,4-diaminotoluene (DAT) in the Salmonella mutagenicity assay was increased with liver fractions from phenobarbital (PB) or beta-naphthoflavone (BNF) treated rats. Substitutions of the hydrogens in the methyl group of 2,4-DAT with deuterium resulted in a decrease in mutagenicity. Incubation of rat liver microsomes with tritiated 2,4-DAT in the presence of NADPH led to the formation of irreversibly bound products to microsomal protein. The rates of binding were not increased using microsomes from PB or BNF-treated rats and was not altered by deuterium substitution in the methyl group. Addition of superoxide dismutase, glutathione (GSH) or rat liver supernatant reduced 2,4-DAT irreversible binding, whereas 2,4-DAT mutagenicity was unaffected by superoxide dismutase addition. Injection of tritiated 2,4-DAT 100 mg/kg to rats lead to its irreversible binding to liver protein and ribosomal RNA and to kidney protein in vivo, again protein binding was not increased after prior treatment with PB or BNF. No irreversible interaction of tritiated 2,4-DAT with DNA either in vitro or in vivo could be demonstrated."}
{"id": "PMID:466726", "title": "Binding of 6-hydroxymethylbenzo[a]pyrene and 6-acetoxymethylbenzo[a]pyrene to DNA.", "content": "The carcinogenic hydrocarbons 6-hydroxymethylbenzo[a]pyrene (6-HOCH2-B[a]P) and 6-acetoxymethylbenzo[a]pyrene (6-AcOCH2-B[a]P) were examined for their ability to bind to rat and calf thymus DNA. The data indicate there are no appreciable differences in the amount of binding to the two types of DNA. Non-enzymatic binding of 6-HOCH2-B[a]P was low (5 mumol hydrocarbon/mol DNA P) but 6-AcOCH2-B[a]P was bound to a considerable extent (88.4--97.3 mumol hydrocarbon/mol DNA P). Non-enzymatic binding of 6-HOCH2-B[a]P was greatly increased in the presence of ATP. Binding of 6-HOCH2-B[a]P in the presence of liver microsomes from untreated rats or from rats pretreated with 3-methylcholanthrene (3-MC) never exceeded 5 mumol hydrocarbon/mol DNA P. Binding of 6-HOCH2-B[a]P in the presence of a PAPS generating system was less than non-enzymatic binding mediated by ATP and was dependent on the presence of ATP rather than ATP and sulfate. Binding was reduced by 50%  when ADP was employed in the non-enzymatic reaction and was negligible in the presence of AMP or adenosine, indicating that a diphosphate group is necessary. Incubation of 6-HOCH2-B[a]P with DNA in the presence of ATP, CTP, GTP, or UTP showed that ATP was the most effective mediator of the binding reaction. These observations suggest that 6-HOCH2-B[a]P is converted to a phosphate ester which, like 6-AcOCH2-B[a]P, is much more reactive than 6-HOCH2-B[a]P itself.", "contents": "Binding of 6-hydroxymethylbenzo[a]pyrene and 6-acetoxymethylbenzo[a]pyrene to DNA. The carcinogenic hydrocarbons 6-hydroxymethylbenzo[a]pyrene (6-HOCH2-B[a]P) and 6-acetoxymethylbenzo[a]pyrene (6-AcOCH2-B[a]P) were examined for their ability to bind to rat and calf thymus DNA. The data indicate there are no appreciable differences in the amount of binding to the two types of DNA. Non-enzymatic binding of 6-HOCH2-B[a]P was low (5 mumol hydrocarbon/mol DNA P) but 6-AcOCH2-B[a]P was bound to a considerable extent (88.4--97.3 mumol hydrocarbon/mol DNA P). Non-enzymatic binding of 6-HOCH2-B[a]P was greatly increased in the presence of ATP. Binding of 6-HOCH2-B[a]P in the presence of liver microsomes from untreated rats or from rats pretreated with 3-methylcholanthrene (3-MC) never exceeded 5 mumol hydrocarbon/mol DNA P. Binding of 6-HOCH2-B[a]P in the presence of a PAPS generating system was less than non-enzymatic binding mediated by ATP and was dependent on the presence of ATP rather than ATP and sulfate. Binding was reduced by 50%  when ADP was employed in the non-enzymatic reaction and was negligible in the presence of AMP or adenosine, indicating that a diphosphate group is necessary. Incubation of 6-HOCH2-B[a]P with DNA in the presence of ATP, CTP, GTP, or UTP showed that ATP was the most effective mediator of the binding reaction. These observations suggest that 6-HOCH2-B[a]P is converted to a phosphate ester which, like 6-AcOCH2-B[a]P, is much more reactive than 6-HOCH2-B[a]P itself."}
{"id": "PMID:466727", "title": "Comparative cytotoxic and antitumoral effects of ellipticine derivatives on mouse L 1210 leukemia.", "content": "Twelve derivatives of the antitumoral alkaloid ellipticine (E) and ellipticinium were assayed in vitro on cultured L 1210 cells. These drugs possess varying abilities to decrease the cell growth rate in a 1--1000-fold range. Some of them have a highly cytotoxic effect in the 10(-8)--10(-6) M range. Non-specific intracellular damages are produced: multilobation of nuclei, occurrence of numerous lipid granules, diminution of the size and increase in the number of mitochondrial profiles and several modifications of the internal architecture of mitochondria. 2-Methyl-9-hydroxyellipticinium (2-CH3-9-OHE) was submitted to a bioassay; it inactivates the tumorigenic potency of the cells exposed to it, when they are grafted back into mice in the same dose range which reduces in vitro the growth rate of the cells. A fairly good correlation holds between the in vitro and in vivo (antitumor effect) assays, offering a possible prescreening test for a cheaper and rapid evaluation of chemotherapeutic activity of these compounds. The results stress again the importance of the 9-hydroxy substitution in these series for improving the anticancer efficiency. The nature of the biochemical target of E and derivatives is discussed according to our data.", "contents": "Comparative cytotoxic and antitumoral effects of ellipticine derivatives on mouse L 1210 leukemia. Twelve derivatives of the antitumoral alkaloid ellipticine (E) and ellipticinium were assayed in vitro on cultured L 1210 cells. These drugs possess varying abilities to decrease the cell growth rate in a 1--1000-fold range. Some of them have a highly cytotoxic effect in the 10(-8)--10(-6) M range. Non-specific intracellular damages are produced: multilobation of nuclei, occurrence of numerous lipid granules, diminution of the size and increase in the number of mitochondrial profiles and several modifications of the internal architecture of mitochondria. 2-Methyl-9-hydroxyellipticinium (2-CH3-9-OHE) was submitted to a bioassay; it inactivates the tumorigenic potency of the cells exposed to it, when they are grafted back into mice in the same dose range which reduces in vitro the growth rate of the cells. A fairly good correlation holds between the in vitro and in vivo (antitumor effect) assays, offering a possible prescreening test for a cheaper and rapid evaluation of chemotherapeutic activity of these compounds. The results stress again the importance of the 9-hydroxy substitution in these series for improving the anticancer efficiency. The nature of the biochemical target of E and derivatives is discussed according to our data."}
{"id": "PMID:466728", "title": "Studies on the interaction between steffimycins and DNA.", "content": "The complexes formed between steffimycins and DNA were studied using various physicochemical techniques. The binding process has been followed spectrophotometrically or fluorimetrically. The binding parameters n and K, evaluated according to McGhee and Von Hippel, show a good affinity of these antibiotics for the macromolecule. Flow dichroism measurements showed that in the complex with DNA, the antracycline moiety of the steffimycins is intercalated between two base pairs of the macromolecule. The binding experiments with various polydeoxyribonucleotides and with various DNA samples, having different base pair compositions, suggest that an alternate sequence of A-T, such as that of poly[d(A-T)] . poly[d(A-T)], represents a good receptor site for the binding of steffimycins to DNA. The lack of in vivo activity of these antibiotics is discussed.", "contents": "Studies on the interaction between steffimycins and DNA. The complexes formed between steffimycins and DNA were studied using various physicochemical techniques. The binding process has been followed spectrophotometrically or fluorimetrically. The binding parameters n and K, evaluated according to McGhee and Von Hippel, show a good affinity of these antibiotics for the macromolecule. Flow dichroism measurements showed that in the complex with DNA, the antracycline moiety of the steffimycins is intercalated between two base pairs of the macromolecule. The binding experiments with various polydeoxyribonucleotides and with various DNA samples, having different base pair compositions, suggest that an alternate sequence of A-T, such as that of poly[d(A-T)] . poly[d(A-T)], represents a good receptor site for the binding of steffimycins to DNA. The lack of in vivo activity of these antibiotics is discussed."}
{"id": "PMID:466729", "title": "Interaction of DNA and purine nucleosides with cis-dichlorodiammineplatinum(II) and antimitotic activity of the complexes on meristematic root cells.", "content": "The appropriate experimental conditions for the preparation of complexes of cis-dichlorodiammineplatinum(II) with DNA and with purine nucleosides have been determined which leave negligible amounts of free drug in the solution. Important conformational changes of DNA upon binding to cis-Pt(NH3)2-Cl2 have been evidenced through viscosity, electric birefringence and thermal denaturation experiments. The antimitotic and antitumor activity of the drug was found to be totally inhibited by its binding to DNA and to the purine nucleosides. Enzymic degradation observations on the DNA-cis-Pt(NH3)2Cl2 complexes indicated an important inhibition of the degradation and the absence of release of free drug. The implications of the results in relation with the mode of binding of this compound to DNA, with the choice of carriers for drugs and the mechanism of action of lysosomotropic agents are discussed.", "contents": "Interaction of DNA and purine nucleosides with cis-dichlorodiammineplatinum(II) and antimitotic activity of the complexes on meristematic root cells. The appropriate experimental conditions for the preparation of complexes of cis-dichlorodiammineplatinum(II) with DNA and with purine nucleosides have been determined which leave negligible amounts of free drug in the solution. Important conformational changes of DNA upon binding to cis-Pt(NH3)2-Cl2 have been evidenced through viscosity, electric birefringence and thermal denaturation experiments. The antimitotic and antitumor activity of the drug was found to be totally inhibited by its binding to DNA and to the purine nucleosides. Enzymic degradation observations on the DNA-cis-Pt(NH3)2Cl2 complexes indicated an important inhibition of the degradation and the absence of release of free drug. The implications of the results in relation with the mode of binding of this compound to DNA, with the choice of carriers for drugs and the mechanism of action of lysosomotropic agents are discussed."}
{"id": "PMID:466730", "title": "Isolation and partial characterization of a cadmium-binding protein from the American oyster (Crassostrea virginica).", "content": "American oysters (Crassostrea virginica) were exposed to 0.1 ppm cadmium for 0--15 days in a flowing seawater system and then placed into clean flowing seawater for 24 h prior to sacrifice. Whole oysters were homogenized and a cadmium-binding protein isolated and purified by a process of centrifugation, heat-treatment, Sephadex G-75 chromatography, DEAE cellulose chromatography and disc gel electrophoresis. A highly anionic protein which is not present in control oysters was found to be present in cadmium-exposed animals after 3 days of treatment and to increase in concentration at succeeding time points. The protein does not extensively bind zinc or copper. Amino acid analysis of the purified protein disclosed an amino acid composition characterized by a high percentage of dicarboxylic amino acids and relatively little cysteine.", "contents": "Isolation and partial characterization of a cadmium-binding protein from the American oyster (Crassostrea virginica). American oysters (Crassostrea virginica) were exposed to 0.1 ppm cadmium for 0--15 days in a flowing seawater system and then placed into clean flowing seawater for 24 h prior to sacrifice. Whole oysters were homogenized and a cadmium-binding protein isolated and purified by a process of centrifugation, heat-treatment, Sephadex G-75 chromatography, DEAE cellulose chromatography and disc gel electrophoresis. A highly anionic protein which is not present in control oysters was found to be present in cadmium-exposed animals after 3 days of treatment and to increase in concentration at succeeding time points. The protein does not extensively bind zinc or copper. Amino acid analysis of the purified protein disclosed an amino acid composition characterized by a high percentage of dicarboxylic amino acids and relatively little cysteine."}
{"id": "PMID:466731", "title": "Metabolism of benzo[A]pyrene in hamster embryo cells. Effect of the concentration of benzo[A]pyrene on its metabolism.", "content": "The metabolism of benzo[a]pyrene (BP) by hamster embryo cells was studied. The production of water-soluble metabolites, shown to be conjugates with glucuronic acid, depended on BP concentration. With increased BP concentration the amount of glucuronic acid conjugates increased, but the proportion of conjugates in BP or its metabolites present in the medium decreased. The metabolites extracted with ethylacetate were trans-7,8-dihydrodiol-BP (7,8-dihydrodiol) and trans-9,10-dihydrodiol-BP (9,10-dihydrodiol), but large peaks of phenolic metabolites were found by high pressure liquid chromatography (HPLC) after digesting the medium with beta-glucuronidase. Therefore, BP is metabolized to oxygenated forms, and of these, most of the phenolic metabolites and parts of the dihydrodiols are conjugated with glucuronic acid. The proportions of dihydrodiols to phenols, estimated by HPLC after beta-glucuronidase digestion, decreased when the BP concentration was decreased. The results suggest that dihydrodiols are less readily glucuronidated than phenols and so may be metabolized further to metabolites other than glucuronic acid conjugates.", "contents": "Metabolism of benzo[A]pyrene in hamster embryo cells. Effect of the concentration of benzo[A]pyrene on its metabolism. The metabolism of benzo[a]pyrene (BP) by hamster embryo cells was studied. The production of water-soluble metabolites, shown to be conjugates with glucuronic acid, depended on BP concentration. With increased BP concentration the amount of glucuronic acid conjugates increased, but the proportion of conjugates in BP or its metabolites present in the medium decreased. The metabolites extracted with ethylacetate were trans-7,8-dihydrodiol-BP (7,8-dihydrodiol) and trans-9,10-dihydrodiol-BP (9,10-dihydrodiol), but large peaks of phenolic metabolites were found by high pressure liquid chromatography (HPLC) after digesting the medium with beta-glucuronidase. Therefore, BP is metabolized to oxygenated forms, and of these, most of the phenolic metabolites and parts of the dihydrodiols are conjugated with glucuronic acid. The proportions of dihydrodiols to phenols, estimated by HPLC after beta-glucuronidase digestion, decreased when the BP concentration was decreased. The results suggest that dihydrodiols are less readily glucuronidated than phenols and so may be metabolized further to metabolites other than glucuronic acid conjugates."}
{"id": "PMID:466733", "title": "Binding of products originating from the peroxidation of liver microsomal lipids to the non-lipid constituents of the microsomal membrane.", "content": "The binding of products derived from the peroxidation of liver microsomal lipids to the non-lipid constituents of the microsomes was studied. To this end arachidonic acid labelled with tritium at the positions of the double bonds was given to rats and allowed to incorporate into the membrane lipids of the liver cell. When liver microsomes containing labelled arachidonic acid were incubated aerobically in the NADPH-dependent system, a marked production of malonic dialdehyde (MDA) occurred and, concomitantly, there was a consistent release of radioactivity from the microsomes into the incubation medium. The addition of EDTA to the incubation medium prevented, to a large extent, both the MDA formation and the release of radioactivity. Chromatographic studies showed that the bulk of the radioactivity released from the incubated microsomes is not MDA. In the incubated microsomes, the radioactivity decreased in total lipids, while it increased by about 15 times in the non-lipoidal residue. A similar increase in radioactivity was seen in microsomal protein, while no increase was observed in microsomal RNA (the radioactivity was negligible in both the incubated and the non-incubated samples). It seems therefore that products originating from lipoperoxidation of arachidonic acid covalently bind to the microsomal protein. In order to investigate whether alterations similar to those observed in the in vitro peroxidation of liver microsomes could be detected in the in vivo intoxication with carbon tetrachloride, rats given labelled arachidonic acid as above, were poisoned with CCl4. Sixty minutes after poisoning, the radioactivity present in the microsomal lipids was generally lower in the intoxicated rats than in the controls, while the labelling of the non-lipoidal residue and of the protein was higher in the CCl4-poisoned rats.", "contents": "Binding of products originating from the peroxidation of liver microsomal lipids to the non-lipid constituents of the microsomal membrane. The binding of products derived from the peroxidation of liver microsomal lipids to the non-lipid constituents of the microsomes was studied. To this end arachidonic acid labelled with tritium at the positions of the double bonds was given to rats and allowed to incorporate into the membrane lipids of the liver cell. When liver microsomes containing labelled arachidonic acid were incubated aerobically in the NADPH-dependent system, a marked production of malonic dialdehyde (MDA) occurred and, concomitantly, there was a consistent release of radioactivity from the microsomes into the incubation medium. The addition of EDTA to the incubation medium prevented, to a large extent, both the MDA formation and the release of radioactivity. Chromatographic studies showed that the bulk of the radioactivity released from the incubated microsomes is not MDA. In the incubated microsomes, the radioactivity decreased in total lipids, while it increased by about 15 times in the non-lipoidal residue. A similar increase in radioactivity was seen in microsomal protein, while no increase was observed in microsomal RNA (the radioactivity was negligible in both the incubated and the non-incubated samples). It seems therefore that products originating from lipoperoxidation of arachidonic acid covalently bind to the microsomal protein. In order to investigate whether alterations similar to those observed in the in vitro peroxidation of liver microsomes could be detected in the in vivo intoxication with carbon tetrachloride, rats given labelled arachidonic acid as above, were poisoned with CCl4. Sixty minutes after poisoning, the radioactivity present in the microsomal lipids was generally lower in the intoxicated rats than in the controls, while the labelling of the non-lipoidal residue and of the protein was higher in the CCl4-poisoned rats."}
{"id": "PMID:466732", "title": "The effects of 1,1-di(p-chlorophenyl)-2-chloroethylene on plasma enzymes and blood constituents in the Japanese quail.", "content": "Glutamate oxaloacetate transminase (GOT), glutamate dehydrogenase (GDH), sorbitol dehydrogenase (SDH), pseudo-cholinesterase (ChE) and various blood constituents were measured in the plasma of Japanese quail fed 1,1-di(p-chlorophenyl)-2-chloroethylene (DDMU) at low levels for periods ranging from 2 to 32 days. Previous work has shown that DDMU is a potent inducer of hepatic microsomal enzymes causing marked structural changes in the liver. A rapid increase in plasma GOT was observed within 4 days accompanied by an increase in relative liver weight. Plasma GDH and SDH increased to a maximum between 16 and 24 dyas which seems to be associated with hepatic cell proliferation. Plasma ChE showed a steady increase over the time course of DDMU administration. The level of plasma lipid was reduced after 4 days whereas the hepatic lipid content was substantially increased suggesting that the fatty liver condition may be caused by decreased release of triglyceride from the liver. Plasma glucose was reduced at 8 days but there was no evidence of a hyperglycaemic state. The changes noted after 2 days of DDMU diet were confirmed by measurements on birds 18 h after oral dosing the DDMU. The study demonstrates the value of plasma enzyme measurements for the early detection of toxic effects and indicates that DDMU administration leads to extrahepatic effects in addition to those previously described in the liver.", "contents": "The effects of 1,1-di(p-chlorophenyl)-2-chloroethylene on plasma enzymes and blood constituents in the Japanese quail. Glutamate oxaloacetate transminase (GOT), glutamate dehydrogenase (GDH), sorbitol dehydrogenase (SDH), pseudo-cholinesterase (ChE) and various blood constituents were measured in the plasma of Japanese quail fed 1,1-di(p-chlorophenyl)-2-chloroethylene (DDMU) at low levels for periods ranging from 2 to 32 days. Previous work has shown that DDMU is a potent inducer of hepatic microsomal enzymes causing marked structural changes in the liver. A rapid increase in plasma GOT was observed within 4 days accompanied by an increase in relative liver weight. Plasma GDH and SDH increased to a maximum between 16 and 24 dyas which seems to be associated with hepatic cell proliferation. Plasma ChE showed a steady increase over the time course of DDMU administration. The level of plasma lipid was reduced after 4 days whereas the hepatic lipid content was substantially increased suggesting that the fatty liver condition may be caused by decreased release of triglyceride from the liver. Plasma glucose was reduced at 8 days but there was no evidence of a hyperglycaemic state. The changes noted after 2 days of DDMU diet were confirmed by measurements on birds 18 h after oral dosing the DDMU. The study demonstrates the value of plasma enzyme measurements for the early detection of toxic effects and indicates that DDMU administration leads to extrahepatic effects in addition to those previously described in the liver."}
{"id": "PMID:466734", "title": "Persistence and accumulation of (potential) single strand breaks in liver DNA of rats treated with diethylnitrosamine or dimethylnitrosamine: correlation with hepatocarcinogenicity.", "content": "Effects of diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) on the sedimentation pattern of [3H]thymidine-labelled Sprague-Dawley female rat liver DNA in alkaline sucrose gradients were studied with regard to time and dose dependency. In experiments at 1--56 days after a single injection it was observed that (potential) single strand breaks induced by DEN were repaired at a low rate. At 56 days the sedimentation pattern was still grossly abnormal. Half-life values of 27 and 46 days were observed after 134 mg/kg DEN (approx. 45% of the LD50) and 13.4 mg/kg DEN, respectively. Identical experiments after DMN (10 mg/kg, corresponding to about 35% of the LD50) showed return to (almost) completely control sedimentation patterns within 56 days after injection (t 1/2 = 8 days). Experiments at 6 or 56 days after the last of a series of 5 or 10 weekly injections of DEN (13.4 mg/kg) showed that a major part of DEN-induced damage (measured as single strand breaks) is of a persistent and accumulating character. No accumulation of DMN-induced rat liver lesions was observed. It is concluded that DNA fragmentation and lack of DNA repair is not a consequence of hepatotoxicity. Since at equimolar doses DEN gives appreciably less DNA alkylation (including O6-alkylguanine) but is much more effective both as an inducer of preneoplastic liver lesions and as a hepatocarcinogen when compared with DMN, we believe that the formation of persistent (and accumulating) DNA damage after DEN administration might be relevant in the process of liver tumour formation.", "contents": "Persistence and accumulation of (potential) single strand breaks in liver DNA of rats treated with diethylnitrosamine or dimethylnitrosamine: correlation with hepatocarcinogenicity. Effects of diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) on the sedimentation pattern of [3H]thymidine-labelled Sprague-Dawley female rat liver DNA in alkaline sucrose gradients were studied with regard to time and dose dependency. In experiments at 1--56 days after a single injection it was observed that (potential) single strand breaks induced by DEN were repaired at a low rate. At 56 days the sedimentation pattern was still grossly abnormal. Half-life values of 27 and 46 days were observed after 134 mg/kg DEN (approx. 45% of the LD50) and 13.4 mg/kg DEN, respectively. Identical experiments after DMN (10 mg/kg, corresponding to about 35% of the LD50) showed return to (almost) completely control sedimentation patterns within 56 days after injection (t 1/2 = 8 days). Experiments at 6 or 56 days after the last of a series of 5 or 10 weekly injections of DEN (13.4 mg/kg) showed that a major part of DEN-induced damage (measured as single strand breaks) is of a persistent and accumulating character. No accumulation of DMN-induced rat liver lesions was observed. It is concluded that DNA fragmentation and lack of DNA repair is not a consequence of hepatotoxicity. Since at equimolar doses DEN gives appreciably less DNA alkylation (including O6-alkylguanine) but is much more effective both as an inducer of preneoplastic liver lesions and as a hepatocarcinogen when compared with DMN, we believe that the formation of persistent (and accumulating) DNA damage after DEN administration might be relevant in the process of liver tumour formation."}
{"id": "PMID:466735", "title": "Studies on the distribution and metabolism of N-[14C]nitrosopyrrolidine in mice.", "content": "Pretreatments with pyrazole, ethanol, nialamide or diethyldithiocarbamate were found to strongly depress the exhalation of 14CO2 and the incorporation of radioactivity in the acid-insoluble fraction of the liver in mice injected with N-[14C]nitrosopyrrolidine. Whole-body autoradiography performed with hemisections of mice at -80 degrees C (to prevent evaporation of the volatile N-nitrosopyrrolidine) and with dry tape-sections (to localize the non-volatile metabolites), using pretreated and non-pretreated mice, indicated a uniform distribution of the non-metabolized N-nitrosopyrrolidine in the tissues. At the shortest survival intervals (1 and 5 min), a high level of metabolites were found in the liver, the tracheo-bronchial and nasal mucosa and Harder's gland, indicating a local formation of metabolits in these tissues. At later survival intervals (0.5--24 h) metabolites were in addition found in tissues with a rapid cell turnover and a high rate of protein synthesis and in brown fat, which probably reflects incorporation of metabolites via normal biosynthetic pathways. Autoradiography of N-[14C]nitrosopyrrolidine in mice given the substance orally resulted in distribution pictures similar to those obtained after i.v. injections.", "contents": "Studies on the distribution and metabolism of N-[14C]nitrosopyrrolidine in mice. Pretreatments with pyrazole, ethanol, nialamide or diethyldithiocarbamate were found to strongly depress the exhalation of 14CO2 and the incorporation of radioactivity in the acid-insoluble fraction of the liver in mice injected with N-[14C]nitrosopyrrolidine. Whole-body autoradiography performed with hemisections of mice at -80 degrees C (to prevent evaporation of the volatile N-nitrosopyrrolidine) and with dry tape-sections (to localize the non-volatile metabolites), using pretreated and non-pretreated mice, indicated a uniform distribution of the non-metabolized N-nitrosopyrrolidine in the tissues. At the shortest survival intervals (1 and 5 min), a high level of metabolites were found in the liver, the tracheo-bronchial and nasal mucosa and Harder's gland, indicating a local formation of metabolits in these tissues. At later survival intervals (0.5--24 h) metabolites were in addition found in tissues with a rapid cell turnover and a high rate of protein synthesis and in brown fat, which probably reflects incorporation of metabolites via normal biosynthetic pathways. Autoradiography of N-[14C]nitrosopyrrolidine in mice given the substance orally resulted in distribution pictures similar to those obtained after i.v. injections."}
{"id": "PMID:466736", "title": "Preferential S-sulfonate formation in lung and aorta.", "content": "S-sulfonate (S-SO-3) compounds have previously been identified as metabolites of sulfite in the plasma of several species of mammals [6--8]. We now report the formation of non-diffusible and relatively stable S-sulfonates in the aorta and lung lobes of rabbits exposed intravenously to constant arterial sulfite concentrations of approx. 550 microM. Under these conditions the kinetics of S-SO-3 formation were first order with coefficients in the range of 0.3--0.4 h-1 and asymptotic concentrations of approx. 900 and 9000 nmol S-SO-3/g dry wt. of lung and aorta respectively. The kinetics of this reaction in aorta tissue were closely approximated in vitro. Clearance of S-SO-3 from both lungs and aorta appeared to be first order with a half-life of 2--3 days.", "contents": "Preferential S-sulfonate formation in lung and aorta. S-sulfonate (S-SO-3) compounds have previously been identified as metabolites of sulfite in the plasma of several species of mammals [6--8]. We now report the formation of non-diffusible and relatively stable S-sulfonates in the aorta and lung lobes of rabbits exposed intravenously to constant arterial sulfite concentrations of approx. 550 microM. Under these conditions the kinetics of S-SO-3 formation were first order with coefficients in the range of 0.3--0.4 h-1 and asymptotic concentrations of approx. 900 and 9000 nmol S-SO-3/g dry wt. of lung and aorta respectively. The kinetics of this reaction in aorta tissue were closely approximated in vitro. Clearance of S-SO-3 from both lungs and aorta appeared to be first order with a half-life of 2--3 days."}
{"id": "PMID:466737", "title": "Epoxide hydrase and glutathione S-transferase activities with selected alkene and adrene oxides in several marine species.", "content": "Epoxide hydrase and glutathione (GSH) S-transferase activities were measured in subcellular fractions prepared from liver or hepatopancreas and some extrahepatic organs of a number of marine species common to Maine or Florida. These activities were easily detected in the species studied. In fish, hepatic GSH S-transferase activities were normally higher than hepatic epoxide hydrase activities for the alkene oxide (styrene oxide and octene oxide) and arene oxide (benzo[a]pyrene 4,5-oxide) substrates studied, whereas in crustacea, hepatopancreas epoxide hydrase activities were higher than hepatopancreas GSH S-transferase activities with the same substrates. Extrahepatic organs from fish and crustacea usually had higher GSH S-transferase activities than epoxide hydrase activities with the alkene and arene oxide substrates. GSH S-transferase activity was also found in liver or hepatopancreas of every aquatic species studied and in a number of extrahepatic organs, when 1,2-dichloro-4-nitrobenzene or 1-chloro-2,4-dinitrobenzene served as substrate.", "contents": "Epoxide hydrase and glutathione S-transferase activities with selected alkene and adrene oxides in several marine species. Epoxide hydrase and glutathione (GSH) S-transferase activities were measured in subcellular fractions prepared from liver or hepatopancreas and some extrahepatic organs of a number of marine species common to Maine or Florida. These activities were easily detected in the species studied. In fish, hepatic GSH S-transferase activities were normally higher than hepatic epoxide hydrase activities for the alkene oxide (styrene oxide and octene oxide) and arene oxide (benzo[a]pyrene 4,5-oxide) substrates studied, whereas in crustacea, hepatopancreas epoxide hydrase activities were higher than hepatopancreas GSH S-transferase activities with the same substrates. Extrahepatic organs from fish and crustacea usually had higher GSH S-transferase activities than epoxide hydrase activities with the alkene and arene oxide substrates. GSH S-transferase activity was also found in liver or hepatopancreas of every aquatic species studied and in a number of extrahepatic organs, when 1,2-dichloro-4-nitrobenzene or 1-chloro-2,4-dinitrobenzene served as substrate."}
{"id": "PMID:466738", "title": "In vivo alkylation of foetal, maternal and normal rat tissue nucleic acids by 3-methyl-1-phenyltriazene.", "content": "The carcinogen 3-methyl-1-phenyltriazene (MPT) was administered subcutaneously to normal or pregnant BD VI rats and DNA and RNA were isolated from various tissues after 8 h or 15 h, respectively. Sephadex G-10 chromatography of DNA hydrolysates showed the presence of 7-methylguanine in all tissues examined including that of the brain, one of the target organs for tumour induction. The amounts of the minor product, O6-methylguanine, were characteristic of an SN1 reaction mechanism. Dowex-50 chromatography of RNA hydrolysates showed the presence of 7-methylguanine and of the minor product, 3-methylcytosine. The relative amounts, both of the methylated bases in the individual nucleic acids and of 7-methylguanine in DNA and RNA, were similar to those found previously after administration of 3,3-dimethyl-1-phenyltriazene (DMPT). This suggests the involvment of a common alkylating intermediate. De novo incorporation of radioactivity into purine bases was detected in both DNA and RNA although the levels were not related to the amounts of methylation. The results show that MPT is sufficiently stable to alkylate nucleic acids in vivo and are consistent with the hypothesis that this reaction is a prerequisite for tumour induction. Futhermore, they support the proposal that MPT is the active intermediate in the induction of tumours by DMPT.", "contents": "In vivo alkylation of foetal, maternal and normal rat tissue nucleic acids by 3-methyl-1-phenyltriazene. The carcinogen 3-methyl-1-phenyltriazene (MPT) was administered subcutaneously to normal or pregnant BD VI rats and DNA and RNA were isolated from various tissues after 8 h or 15 h, respectively. Sephadex G-10 chromatography of DNA hydrolysates showed the presence of 7-methylguanine in all tissues examined including that of the brain, one of the target organs for tumour induction. The amounts of the minor product, O6-methylguanine, were characteristic of an SN1 reaction mechanism. Dowex-50 chromatography of RNA hydrolysates showed the presence of 7-methylguanine and of the minor product, 3-methylcytosine. The relative amounts, both of the methylated bases in the individual nucleic acids and of 7-methylguanine in DNA and RNA, were similar to those found previously after administration of 3,3-dimethyl-1-phenyltriazene (DMPT). This suggests the involvment of a common alkylating intermediate. De novo incorporation of radioactivity into purine bases was detected in both DNA and RNA although the levels were not related to the amounts of methylation. The results show that MPT is sufficiently stable to alkylate nucleic acids in vivo and are consistent with the hypothesis that this reaction is a prerequisite for tumour induction. Futhermore, they support the proposal that MPT is the active intermediate in the induction of tumours by DMPT."}
{"id": "PMID:466740", "title": "Interaction of benzo [A] pyrene and a hyperplastic agent in epidermal nuclear enlargement in the mouse. A dose response study.", "content": "Experiments were conducted on the effects of various dose levels of benzo [a]pyrene (BP) on nuclear size in mouse interfollicular epidermis over a 3-day period. Topical application of BP was made with or without croton oil (CO) (0.1 or 0.5%) in the vehicles acetone, toluene and methyl ethyl ketone (MEK). Nuclear size was measured on histological sections either manually or by Quantimet Image Analyser. Vehicle controls treated with 0.1 or 0.5% CO in acetone or MEK gave rise to epidermal hyperplasia with some nuclear enlargement and toluene without CO produced a similar response. It was found that when BP was applied in a vehicle capable of inducing hyperplasia, the nuclear enlargement produced was greater than that produced by either the vehicle control or BP in a non-irritant vehicle. The enhancement of response to BP when tested in the presence of a hyperplastic agent resulted in lower concentrations of BP being detectable. As the levels of BP detectable by nuclear enlargement under these conditions compared reasonably well with those detectable in long-term tests, this system might be usable as a basis for a short-term test for carcinogens.", "contents": "Interaction of benzo [A] pyrene and a hyperplastic agent in epidermal nuclear enlargement in the mouse. A dose response study. Experiments were conducted on the effects of various dose levels of benzo [a]pyrene (BP) on nuclear size in mouse interfollicular epidermis over a 3-day period. Topical application of BP was made with or without croton oil (CO) (0.1 or 0.5%) in the vehicles acetone, toluene and methyl ethyl ketone (MEK). Nuclear size was measured on histological sections either manually or by Quantimet Image Analyser. Vehicle controls treated with 0.1 or 0.5% CO in acetone or MEK gave rise to epidermal hyperplasia with some nuclear enlargement and toluene without CO produced a similar response. It was found that when BP was applied in a vehicle capable of inducing hyperplasia, the nuclear enlargement produced was greater than that produced by either the vehicle control or BP in a non-irritant vehicle. The enhancement of response to BP when tested in the presence of a hyperplastic agent resulted in lower concentrations of BP being detectable. As the levels of BP detectable by nuclear enlargement under these conditions compared reasonably well with those detectable in long-term tests, this system might be usable as a basis for a short-term test for carcinogens."}
{"id": "PMID:466741", "title": "Dose and effect of methyl-2-benzimidazolylcarbamate in the \"mammalian spot test\", an in vivo method for the detection of genetic alterations in somatic cells of mice.", "content": "In the spot test, mouse embryos which are heterozygous for four different recessive coat-colour genes are treated in utero by injection of a mutagen into the peritoneal cavity of the mother or by other appropriate routes of administration. If this treatment leads in a pigment precursor cell to an alteration of the wild type allele of one of the genes under study or to its loss, a colour spot in the adult coat may be seen. Peroral application of 100-300 mg methyl-2-benzimidazolylcarbamate (MBC)/kg to the mother during the tenth day postconception led to an increase in the frequency of colour spots in the coats of offspring. The data are consistent with the hypothesis that MBC is a point mutagen.", "contents": "Dose and effect of methyl-2-benzimidazolylcarbamate in the \"mammalian spot test\", an in vivo method for the detection of genetic alterations in somatic cells of mice. In the spot test, mouse embryos which are heterozygous for four different recessive coat-colour genes are treated in utero by injection of a mutagen into the peritoneal cavity of the mother or by other appropriate routes of administration. If this treatment leads in a pigment precursor cell to an alteration of the wild type allele of one of the genes under study or to its loss, a colour spot in the adult coat may be seen. Peroral application of 100-300 mg methyl-2-benzimidazolylcarbamate (MBC)/kg to the mother during the tenth day postconception led to an increase in the frequency of colour spots in the coats of offspring. The data are consistent with the hypothesis that MBC is a point mutagen."}
{"id": "PMID:466742", "title": "Reactions of the carcinogen N-acetoxy-4-acetamidostilbene with nucleosides.", "content": "The carcinogen N-acetoxy-4-acetamidostilbene (N-AcO-AAS) yields multiple products in reactions with guanosine, adenosine or cytidine in aqueous acetone. The major product from the reaction with cytidine is a deamination product, 1-(4-acetamidophenyl)-1-(3-uridyl)-2-hydrosy-2-phenylethane. Three minor products were unstable and were characterized only by their UV spectra and pK values. Adenosine yielded two major products, one of them 1-(4-acetamidophenyl)-1-(N6-adenoxyl)-2-hydroxy-2-phenylethane, and the second 3-(beta-D-ribosyl)-7-phenyl-8-(4-acetamidophenyl)-7,8 dihydroimidazo [2,1-i] purine. The major adduct with guanosine is 1-(4-acetamidophenyl)-1-(1-guanosyl)-2-hydroxy-2-phenylethane. One minor adduct also appears to be a guanosine-N-1 derivative, while two other minor adducts yield 1-(4-acetamidophenyl)-2-phenyl-1, 2-ethanediol on acid hydrolysis, and thus appear to be O6-derivatives. None of the guanine adducts isolated had the properties of N-7, C-8 or N2 adducts. In this respect, N-Aco-AAS appears to behave more like a classical alkylating agent than like previously studied N-acetoxy-N-arylacetamides, although the target organs of 4-acetamidostilbene are the same as those of other N-arylacetamides.", "contents": "Reactions of the carcinogen N-acetoxy-4-acetamidostilbene with nucleosides. The carcinogen N-acetoxy-4-acetamidostilbene (N-AcO-AAS) yields multiple products in reactions with guanosine, adenosine or cytidine in aqueous acetone. The major product from the reaction with cytidine is a deamination product, 1-(4-acetamidophenyl)-1-(3-uridyl)-2-hydrosy-2-phenylethane. Three minor products were unstable and were characterized only by their UV spectra and pK values. Adenosine yielded two major products, one of them 1-(4-acetamidophenyl)-1-(N6-adenoxyl)-2-hydroxy-2-phenylethane, and the second 3-(beta-D-ribosyl)-7-phenyl-8-(4-acetamidophenyl)-7,8 dihydroimidazo [2,1-i] purine. The major adduct with guanosine is 1-(4-acetamidophenyl)-1-(1-guanosyl)-2-hydroxy-2-phenylethane. One minor adduct also appears to be a guanosine-N-1 derivative, while two other minor adducts yield 1-(4-acetamidophenyl)-2-phenyl-1, 2-ethanediol on acid hydrolysis, and thus appear to be O6-derivatives. None of the guanine adducts isolated had the properties of N-7, C-8 or N2 adducts. In this respect, N-Aco-AAS appears to behave more like a classical alkylating agent than like previously studied N-acetoxy-N-arylacetamides, although the target organs of 4-acetamidostilbene are the same as those of other N-arylacetamides."}
{"id": "PMID:466743", "title": "Reactions of the carcinogen N-acetoxy-4-acetamidostilbene with polynucleotides in vitro.", "content": "N-Acetoxy-4-acetamidostilbene (N-AcO-AAS) has been shown to react with mononucleosides to give numerous alkylation products [1]. In this work, homopolynucleotides, RNA and DNA were treated with N-[beta-14 C]-AcO-AAS, washed, degraded with S1 nuclease and acid phosphatase, and chromatographed on Sephadex LH-20. RNA prepared in vitro with 14C on cytosine, adenine or guanine was treated with non-radioactive N-AcO-AAS, then digested and chromatographed similarly. By this means, many of the adducts rising from nucleoside reactions were shown to result from treatment of nucleic acids with the same carcinogen, as well as a number of products which have not been matched to products of monomer alkylation. Labeled 1-(4-acetamidophenyl)-2-phenyl-1, 2-ethanediol was detected in the digest of RNA treated with radioactive N-AcO-AAS, suggesting that phosphate alkylation had taken place.", "contents": "Reactions of the carcinogen N-acetoxy-4-acetamidostilbene with polynucleotides in vitro. N-Acetoxy-4-acetamidostilbene (N-AcO-AAS) has been shown to react with mononucleosides to give numerous alkylation products [1]. In this work, homopolynucleotides, RNA and DNA were treated with N-[beta-14 C]-AcO-AAS, washed, degraded with S1 nuclease and acid phosphatase, and chromatographed on Sephadex LH-20. RNA prepared in vitro with 14C on cytosine, adenine or guanine was treated with non-radioactive N-AcO-AAS, then digested and chromatographed similarly. By this means, many of the adducts rising from nucleoside reactions were shown to result from treatment of nucleic acids with the same carcinogen, as well as a number of products which have not been matched to products of monomer alkylation. Labeled 1-(4-acetamidophenyl)-2-phenyl-1, 2-ethanediol was detected in the digest of RNA treated with radioactive N-AcO-AAS, suggesting that phosphate alkylation had taken place."}
{"id": "PMID:466744", "title": "Comparison of aflatoxin B1 and aflatoxin G1 binding to cellular macromolecules in vitro, in vivo and after peracid oxidation; characterisation of the major nucleic acid adducts.", "content": "A comparison between [14C]aflatoxin B1 (AFB1) and [14C]aflatoxin G1 (AFG1) binding to rat liver and kidney cellular macromolecules has shown AFG1-DNA and-ribosomal RNA binding to be lower in both organs. For both mycotoxins more was bound to nucleic acids than to protein. Two hours after intraperitoneal injection (60 microgram/100 g) of [14C] AFB1, 40 ng, 151 ng/mg. Loss of radioactivity bound to liver DNA for both [14C]AFB1 and protein respectively and for [14C]AFG1 the respective figures were 10, 7 and 1 ng/mg. Loss of liver bound radioactivity to DNA for both [14C]AFG1 and [14C]AFG1 appeared to be biphasic indicating that an enzymic DNA repair process may be operating. In vitro binding studies also showed less AFG1 was bound to exogenous DNA after microsomal activation than AFB1. This difference was not a result of differences in the chemical reactivity of the \"ultimate\" electrophilic species, the respective expoxides, since chemical activation studies using 3-chloroperbenzoic acid showed similar amounts of AFG1 and AFB1 to be converted to the epoxides and to bind to DNA. Studies on the distribution coefficients of the two mycotoxins showed AFB1 to be more lipophilic than AFG1 and this may be an important factor in determining the weaker carcinogenicity of the latter compound. Characterisation of the major AFG1-DNA adduct formed in vitro, in vivo and after peracid oxidation showed it to have the structure trans-9,10-dihydro-9-(7-guanyl)-10-hydroxy-aflatoxin G1. This adduct is similar to that obtained from AFB1 by activation in vivo, in vitro and after peracid oxidation.", "contents": "Comparison of aflatoxin B1 and aflatoxin G1 binding to cellular macromolecules in vitro, in vivo and after peracid oxidation; characterisation of the major nucleic acid adducts. A comparison between [14C]aflatoxin B1 (AFB1) and [14C]aflatoxin G1 (AFG1) binding to rat liver and kidney cellular macromolecules has shown AFG1-DNA and-ribosomal RNA binding to be lower in both organs. For both mycotoxins more was bound to nucleic acids than to protein. Two hours after intraperitoneal injection (60 microgram/100 g) of [14C] AFB1, 40 ng, 151 ng/mg. Loss of radioactivity bound to liver DNA for both [14C]AFB1 and protein respectively and for [14C]AFG1 the respective figures were 10, 7 and 1 ng/mg. Loss of liver bound radioactivity to DNA for both [14C]AFG1 and [14C]AFG1 appeared to be biphasic indicating that an enzymic DNA repair process may be operating. In vitro binding studies also showed less AFG1 was bound to exogenous DNA after microsomal activation than AFB1. This difference was not a result of differences in the chemical reactivity of the \"ultimate\" electrophilic species, the respective expoxides, since chemical activation studies using 3-chloroperbenzoic acid showed similar amounts of AFG1 and AFB1 to be converted to the epoxides and to bind to DNA. Studies on the distribution coefficients of the two mycotoxins showed AFB1 to be more lipophilic than AFG1 and this may be an important factor in determining the weaker carcinogenicity of the latter compound. Characterisation of the major AFG1-DNA adduct formed in vitro, in vivo and after peracid oxidation showed it to have the structure trans-9,10-dihydro-9-(7-guanyl)-10-hydroxy-aflatoxin G1. This adduct is similar to that obtained from AFB1 by activation in vivo, in vitro and after peracid oxidation."}
{"id": "PMID:466745", "title": "Quantum chemical studies of polycyclic aromatic hydrocarbons and their metabolites: correlations to carcinogenicity.", "content": "In the context of the bay region hypothesis for polycyclic aromatic hydrocarbon (PAH) carcinogenesis, molecular properties were calculated for seventeen polycyclic aromatic hydrocarbons related to (1) intrinsic substrate reactivities towards activating and detoxifying metabolism and (2) the stabilities of the putative carbocation ultimate carcinogens. All-valence electron methods were used, avoiding the inherent difficulties found in the pi-electron methods. The calculated substrate reactivities were found to predict major metabolites successfully, supporting the validity of their use in attempted correlations with observed carcinogenic potencies. Positive correlations were found between observed carcinogenic potencies and (1) the reactivities of the parent polycyclic aromatic hydrocarbons towards the initial distal bay region epoxidation and (2) the stabilities of the diol epoxide carbocations. The reactivities of the distal bay region diol epoxides, were high for both carcinogenic and non-carcinogenic compounds, implying that the second epoxidation does not determine relative carcinogenic activity. Support for a possible alternative hypothesis, that polycyclic aromatic hydrocarbons are activated by one electron oxidation, was also found.", "contents": "Quantum chemical studies of polycyclic aromatic hydrocarbons and their metabolites: correlations to carcinogenicity. In the context of the bay region hypothesis for polycyclic aromatic hydrocarbon (PAH) carcinogenesis, molecular properties were calculated for seventeen polycyclic aromatic hydrocarbons related to (1) intrinsic substrate reactivities towards activating and detoxifying metabolism and (2) the stabilities of the putative carbocation ultimate carcinogens. All-valence electron methods were used, avoiding the inherent difficulties found in the pi-electron methods. The calculated substrate reactivities were found to predict major metabolites successfully, supporting the validity of their use in attempted correlations with observed carcinogenic potencies. Positive correlations were found between observed carcinogenic potencies and (1) the reactivities of the parent polycyclic aromatic hydrocarbons towards the initial distal bay region epoxidation and (2) the stabilities of the diol epoxide carbocations. The reactivities of the distal bay region diol epoxides, were high for both carcinogenic and non-carcinogenic compounds, implying that the second epoxidation does not determine relative carcinogenic activity. Support for a possible alternative hypothesis, that polycyclic aromatic hydrocarbons are activated by one electron oxidation, was also found."}
{"id": "PMID:466746", "title": "The action of rat cytosol enzymes on some methylated nucleic acid components produced by the carcinogenic N-nitroso compounds.", "content": "The stabilities of several alkylated nucleic acid components have been examined in the presence of cytosol extracts from a variety of rat tissues. An activity capable of demethylating O6-methyldeoxyguanosine was readily detectable in all tissues examined; arranged in approximate order of decreasing specific activity these are as follows: small intestine, spleen, kidney, lung, liver, skin, heart and brain. The in vitro requirements for the activity derived from liver and the observations that O6-methylguanine and its deoxynucleoside 5'-monophosphate are insensitive to the action of these extracts suggests that this activity may be due to an enzyme which resembles adenosine deaminase. In contrast to the ready degradation of O6-methyldeoxyguanosine the corresponding ethyl derivative was degraded very much more slowly but there was no evidence for other activities against the O4- and O2-methyldeoxythymidines. Similarly, no demethylation of the N-substituted deoxynucleosides, 3-methyldeoxycytidine 3-methldeoxythymidine, 1-methyldeoxyadenosine and 7-methyldeoxyguanosine, was detected.", "contents": "The action of rat cytosol enzymes on some methylated nucleic acid components produced by the carcinogenic N-nitroso compounds. The stabilities of several alkylated nucleic acid components have been examined in the presence of cytosol extracts from a variety of rat tissues. An activity capable of demethylating O6-methyldeoxyguanosine was readily detectable in all tissues examined; arranged in approximate order of decreasing specific activity these are as follows: small intestine, spleen, kidney, lung, liver, skin, heart and brain. The in vitro requirements for the activity derived from liver and the observations that O6-methylguanine and its deoxynucleoside 5'-monophosphate are insensitive to the action of these extracts suggests that this activity may be due to an enzyme which resembles adenosine deaminase. In contrast to the ready degradation of O6-methyldeoxyguanosine the corresponding ethyl derivative was degraded very much more slowly but there was no evidence for other activities against the O4- and O2-methyldeoxythymidines. Similarly, no demethylation of the N-substituted deoxynucleosides, 3-methyldeoxycytidine 3-methldeoxythymidine, 1-methyldeoxyadenosine and 7-methyldeoxyguanosine, was detected."}
{"id": "PMID:466750", "title": "Atopic disease in ulcerative colitis and Crohn's disease.", "content": "Three hundred patients with ulcerative colitis, 200 with Crohn's disease and matched control subjects completed questionnaires about atopic disease. They were asked whether they had ever suffered from asthma, hay fever, allergic rhinitis or eczema; in ulcerative colitis all of these features occurred with twice the frequency, but in Crohn's disease only eczema was more common than in controls.", "contents": "Atopic disease in ulcerative colitis and Crohn's disease. Three hundred patients with ulcerative colitis, 200 with Crohn's disease and matched control subjects completed questionnaires about atopic disease. They were asked whether they had ever suffered from asthma, hay fever, allergic rhinitis or eczema; in ulcerative colitis all of these features occurred with twice the frequency, but in Crohn's disease only eczema was more common than in controls."}
{"id": "PMID:466751", "title": "'Barn allergy': asthma and rhinitis due to storage mites.", "content": "The differential diagnosis of asthma in farm workers is between a type I hypersensitivity to a pollen or dust allergen and a type III hypersensitivity to mould antigens. We describe asthma and rhinitis, and not farmer's lung, in a farming population due to the non-pyroglyphid grain storage mite. Before a diagnosis of farmers' lung due to mouldy hay is made in any patient whether or not precipitins to Micropolyspora faeni are present, skin tests for storage mite should be made. If these are positive a diagnosis of 'barn allergy' should be considered and a trial of sodium cromoglycate be given.", "contents": "'Barn allergy': asthma and rhinitis due to storage mites. The differential diagnosis of asthma in farm workers is between a type I hypersensitivity to a pollen or dust allergen and a type III hypersensitivity to mould antigens. We describe asthma and rhinitis, and not farmer's lung, in a farming population due to the non-pyroglyphid grain storage mite. Before a diagnosis of farmers' lung due to mouldy hay is made in any patient whether or not precipitins to Micropolyspora faeni are present, skin tests for storage mite should be made. If these are positive a diagnosis of 'barn allergy' should be considered and a trial of sodium cromoglycate be given."}
{"id": "PMID:466747", "title": "What kind of thing is 'being clumsy'?", "content": "The term 'clumsy' is used both as a colloquial pejorative and as a specific diagnostic entity. The meaning of the word and the possible means by which a child may come to be regarded as clumsy are discussed. The implications for management are considered.", "contents": "What kind of thing is 'being clumsy'? The term 'clumsy' is used both as a colloquial pejorative and as a specific diagnostic entity. The meaning of the word and the possible means by which a child may come to be regarded as clumsy are discussed. The implications for management are considered."}
{"id": "PMID:466752", "title": "Mite allergen content in commercial extracts and in bed dust determined by radioallergosorbent tests.", "content": "Radioallergosorbent (RAST) direct binding and inhibition type assays were used to quantitate the mite (Dermatophagoides pteronyssinus) allergen content of four commercial mite extracts and a laboratory prepared extract from freeze-dried mites. The content of mite allergen in extracts prepared from twenty samples of dust vacuumed from bedding was measured by RAST inhibition assay. The four commercial mite extracts designated A, B, C and D, and the laboratory extract, designated L, contained 52, 265, 108, 1.5 and 581 arbitrary units of allergen/ml for the direct binding assay and 128, 111, 217, approximately 1 and 1083 arbitrary, but different, units of allergen/ml for the inhibition assay respectively. Qualitative differences between at least two extracts were suggested by the different slopes obtained when allergen binding of anti IgE was plotted against the volume of extract used in the direct binding assay. Differences in slope between the two extracts were also apparent when they were used in the inhibition assay. The quantities of mite allergen/gm of bed dust expressed in arbitrary units for the inhibition assay were 24 to 457 (mean 129) units. These quantities are similar to and sometimes greater than the quantity in 1 ml of mite extract and so confirm bed dust as a potent source of mite allergen. There was no significant correlation between the weight of dust, the numbers of dead and live mites and the allergen content of dust.", "contents": "Mite allergen content in commercial extracts and in bed dust determined by radioallergosorbent tests. Radioallergosorbent (RAST) direct binding and inhibition type assays were used to quantitate the mite (Dermatophagoides pteronyssinus) allergen content of four commercial mite extracts and a laboratory prepared extract from freeze-dried mites. The content of mite allergen in extracts prepared from twenty samples of dust vacuumed from bedding was measured by RAST inhibition assay. The four commercial mite extracts designated A, B, C and D, and the laboratory extract, designated L, contained 52, 265, 108, 1.5 and 581 arbitrary units of allergen/ml for the direct binding assay and 128, 111, 217, approximately 1 and 1083 arbitrary, but different, units of allergen/ml for the inhibition assay respectively. Qualitative differences between at least two extracts were suggested by the different slopes obtained when allergen binding of anti IgE was plotted against the volume of extract used in the direct binding assay. Differences in slope between the two extracts were also apparent when they were used in the inhibition assay. The quantities of mite allergen/gm of bed dust expressed in arbitrary units for the inhibition assay were 24 to 457 (mean 129) units. These quantities are similar to and sometimes greater than the quantity in 1 ml of mite extract and so confirm bed dust as a potent source of mite allergen. There was no significant correlation between the weight of dust, the numbers of dead and live mites and the allergen content of dust."}
{"id": "PMID:466749", "title": "Action research and evaluation: can research and practice be successfully combined?", "content": "Educative intervention with the young severely subnormal child and his family is envisaged as an example of 'action research' in which the dual roles of the practitioner-researcher and the client-subject are examined. A view of the effect of the interaction of these roles on the intervention and its evaluation is given, and conclusions drawn concerning the nature of evaluation appropriate in future projects of this kind.", "contents": "Action research and evaluation: can research and practice be successfully combined? Educative intervention with the young severely subnormal child and his family is envisaged as an example of 'action research' in which the dual roles of the practitioner-researcher and the client-subject are examined. A view of the effect of the interaction of these roles on the intervention and its evaluation is given, and conclusions drawn concerning the nature of evaluation appropriate in future projects of this kind."}
{"id": "PMID:466748", "title": "Living in one-parent families: attainment at school.", "content": "Data drawn from the National Child Development Study enabled an examination to be made of the school attainment of children who had spent any of their childhood in a one-parent family. Overall, children in one-parent families had lower scores on tests of attainment, but after adjusting for background factors this was no longer the case for the reading test, and the differences on the mathematics test were reduced to non-significance. An examination of particular aspects of these background factors showed that the low income of many of the one-parent families was a substantial part of their social disadvantage associated with low attainment, but this was not the case for their housing conditions. Comparisons within the group of one-parent families revealed little variation. This meant that there was no evidence that either the age at which the child's family broke up nor the reason for the break up (i.e. divorce or widowhood) was important in terms of school performance, although there was a very slight indication that children who had acquired a substitute parent figure had lower test results than those whose parents had remained alone.", "contents": "Living in one-parent families: attainment at school. Data drawn from the National Child Development Study enabled an examination to be made of the school attainment of children who had spent any of their childhood in a one-parent family. Overall, children in one-parent families had lower scores on tests of attainment, but after adjusting for background factors this was no longer the case for the reading test, and the differences on the mathematics test were reduced to non-significance. An examination of particular aspects of these background factors showed that the low income of many of the one-parent families was a substantial part of their social disadvantage associated with low attainment, but this was not the case for their housing conditions. Comparisons within the group of one-parent families revealed little variation. This meant that there was no evidence that either the age at which the child's family broke up nor the reason for the break up (i.e. divorce or widowhood) was important in terms of school performance, although there was a very slight indication that children who had acquired a substitute parent figure had lower test results than those whose parents had remained alone."}
{"id": "PMID:466753", "title": "Intravenous NAB 365 (clenbuterol) and terbutaline in exercise-induced bronchospasm (EIB).", "content": "In a single-blind, placebo-controlled, cross-over study, the effects of intravenous 20 micrograms NAB 365 (clenbuterol) and intravenous 250 microgram terbutaline were compared in nine asthmatic patients with bronchospasm induced by exercise. The exercise consisted of treadmill running. The two beta-adrenergic bronchodilator drugs effectively prevented post-exercise bronchospasm in eight of the nine patients. The effects were superior to those in the untreated group and in the placebo-treated group. Six of the patients treated with terbutaline complained of adverse reactions (tremor, palpitation, tachycardia). No adverse reactions were observed after clenbuterol.", "contents": "Intravenous NAB 365 (clenbuterol) and terbutaline in exercise-induced bronchospasm (EIB). In a single-blind, placebo-controlled, cross-over study, the effects of intravenous 20 micrograms NAB 365 (clenbuterol) and intravenous 250 microgram terbutaline were compared in nine asthmatic patients with bronchospasm induced by exercise. The exercise consisted of treadmill running. The two beta-adrenergic bronchodilator drugs effectively prevented post-exercise bronchospasm in eight of the nine patients. The effects were superior to those in the untreated group and in the placebo-treated group. Six of the patients treated with terbutaline complained of adverse reactions (tremor, palpitation, tachycardia). No adverse reactions were observed after clenbuterol."}
{"id": "PMID:466754", "title": "Analysis of the non-immunological activity of allergen extracts in cutaneous tests.", "content": "The technique proposed by the National Institute for Biological Standards and Control, London (NIBSC, 1977) for the control of allergenic extracts for non-immunological activity in skin tests has proved to be both sensitive and useful. We have observed 'false positive' results to substances such as merthiolate or glycerine and also to lyophilised preparations with sugar, notably glucose, as the carrier substance. Other factors studied in 'false positive' reactions to a given extract were pH, osmotic pressure and the presence of traces of endotoxins or histamine. Individually none of these provided satisfactory explanations for the false positive reactions and the use of antagonists or inhibitors of the release of chemical mediators only confirmed the complexity of the non-immunological reaction and the participation of factors other than histamine.", "contents": "Analysis of the non-immunological activity of allergen extracts in cutaneous tests. The technique proposed by the National Institute for Biological Standards and Control, London (NIBSC, 1977) for the control of allergenic extracts for non-immunological activity in skin tests has proved to be both sensitive and useful. We have observed 'false positive' results to substances such as merthiolate or glycerine and also to lyophilised preparations with sugar, notably glucose, as the carrier substance. Other factors studied in 'false positive' reactions to a given extract were pH, osmotic pressure and the presence of traces of endotoxins or histamine. Individually none of these provided satisfactory explanations for the false positive reactions and the use of antagonists or inhibitors of the release of chemical mediators only confirmed the complexity of the non-immunological reaction and the participation of factors other than histamine."}
{"id": "PMID:466756", "title": "Asthma, aspergilloma and anergy in a patient being treated for pulmonary tuberculosis.", "content": "A twenty-one-year old white girl developed cavitating pulmonary tuberculosis which was complicated by nocturnal asthma, aspergilloma and anergy. In addition she developed systemic symptoms suggestive of a hypersensitivity reaction to the aspergilloma.", "contents": "Asthma, aspergilloma and anergy in a patient being treated for pulmonary tuberculosis. A twenty-one-year old white girl developed cavitating pulmonary tuberculosis which was complicated by nocturnal asthma, aspergilloma and anergy. In addition she developed systemic symptoms suggestive of a hypersensitivity reaction to the aspergilloma."}
{"id": "PMID:466758", "title": "Increased concentrations of free fatty acids and glycerol and altered creatine kinase MM isoenzyme patterns in certain diabetic patients.", "content": "We report unusually high concentrations of free fatty acids and glycerol in sera of patients with adult-onset diabetes, and the accompanying alterations in creatine kinase isoenzyme MM patterns associated with such patients. The serum samples with increased free fatty acids also showed increased electrophoretic movement of the MM isoenzyme on cellulose acetate membranes. Fatty acid concentrations found in such samples averaged 9.88 +/- 5.65 (SD) meq/L and the average glycerol concentration was 153 +/- 115 (SD) mg/L. The serum glycerol concentrations correlated with those of the free fatty acids (r = 0.886, slope = 0.309, intercept = 4.76).", "contents": "Increased concentrations of free fatty acids and glycerol and altered creatine kinase MM isoenzyme patterns in certain diabetic patients. We report unusually high concentrations of free fatty acids and glycerol in sera of patients with adult-onset diabetes, and the accompanying alterations in creatine kinase isoenzyme MM patterns associated with such patients. The serum samples with increased free fatty acids also showed increased electrophoretic movement of the MM isoenzyme on cellulose acetate membranes. Fatty acid concentrations found in such samples averaged 9.88 +/- 5.65 (SD) meq/L and the average glycerol concentration was 153 +/- 115 (SD) mg/L. The serum glycerol concentrations correlated with those of the free fatty acids (r = 0.886, slope = 0.309, intercept = 4.76)."}
{"id": "PMID:466759", "title": "Concentrations of ionic, total, and bound fluoride in plasma.", "content": "We found no significant difference between the means for ionic, bound, and total fluoride concentrations in the plasma of male and female subjects of the same age, living in a community with fluoridated water. When results for the 264 fasting subjects were therefore combined according to age, they indicated that persons over 60 years of age have a significantly higher mean ionic (3.89 mumol/L) and total (6.58 mumol/L) fluoride concentration in plasma than do younger age groups. For younger age groups, means ranged from 2.74 to 3.05 mumol/L for ionic fluoride and from 4.74 to 5.58 mumol/L for total. The bound fluoride concentration was lower in individuals 21 to 30 years of age (1.89 mumol/L) than in older age groups (for whom means ranged from 2.42 to 2.68 mumol/L), but was not significantly different from that of individuals who were younger (2.21 mumol/L). A tendency for the mean ionic fluoride concentration to increase with age was noted, but the concentration was significantly higher than the preceding decade group only in those persons over 60 years of age.", "contents": "Concentrations of ionic, total, and bound fluoride in plasma. We found no significant difference between the means for ionic, bound, and total fluoride concentrations in the plasma of male and female subjects of the same age, living in a community with fluoridated water. When results for the 264 fasting subjects were therefore combined according to age, they indicated that persons over 60 years of age have a significantly higher mean ionic (3.89 mumol/L) and total (6.58 mumol/L) fluoride concentration in plasma than do younger age groups. For younger age groups, means ranged from 2.74 to 3.05 mumol/L for ionic fluoride and from 4.74 to 5.58 mumol/L for total. The bound fluoride concentration was lower in individuals 21 to 30 years of age (1.89 mumol/L) than in older age groups (for whom means ranged from 2.42 to 2.68 mumol/L), but was not significantly different from that of individuals who were younger (2.21 mumol/L). A tendency for the mean ionic fluoride concentration to increase with age was noted, but the concentration was significantly higher than the preceding decade group only in those persons over 60 years of age."}
{"id": "PMID:466760", "title": "Immunochemical determination of human immunoglobulins: use of kinetic turbidimetry and a 36-place centrifugal analyzer.", "content": "We describe the measurement of human immunoglobulins IgG, IgA, and IgM in diluted sera, with diluted commercial monospecific antisera, with use of a 36-place centrifugal analyzer (RotoChem IIa; American Instrument Co., Silver Spring, MD 20920). The assay involves a two-point kinetic turbidimetric technique. The changes in absorbance at 340 nm between 10 and 255 s are taken for standard curve construction with use of a computer-generated cubic least-squares fit. Patients' samples are quickly calculated from the stored curve. Multiple time-interval readings are taken to observe the reaction kinetics. The lowest detectable concentrations are: IgG, 5 mg/L; IgA, 20 mg/L; and IgM, 20 mg/L. Correlation with radial immunodiffusion was excellent. Precision, accuracy, linearity, and sensitivity were very acceptable. Antigen or antibody excess can be easily detected. The accuracy of the proposed method when measuring idiotypic monoclonal proteins is greater than radial immunodiffusion. Up to 30 patients' samples can be analyzed at one time, and calculation of test results by use of the computer program is efficient and rapid.", "contents": "Immunochemical determination of human immunoglobulins: use of kinetic turbidimetry and a 36-place centrifugal analyzer. We describe the measurement of human immunoglobulins IgG, IgA, and IgM in diluted sera, with diluted commercial monospecific antisera, with use of a 36-place centrifugal analyzer (RotoChem IIa; American Instrument Co., Silver Spring, MD 20920). The assay involves a two-point kinetic turbidimetric technique. The changes in absorbance at 340 nm between 10 and 255 s are taken for standard curve construction with use of a computer-generated cubic least-squares fit. Patients' samples are quickly calculated from the stored curve. Multiple time-interval readings are taken to observe the reaction kinetics. The lowest detectable concentrations are: IgG, 5 mg/L; IgA, 20 mg/L; and IgM, 20 mg/L. Correlation with radial immunodiffusion was excellent. Precision, accuracy, linearity, and sensitivity were very acceptable. Antigen or antibody excess can be easily detected. The accuracy of the proposed method when measuring idiotypic monoclonal proteins is greater than radial immunodiffusion. Up to 30 patients' samples can be analyzed at one time, and calculation of test results by use of the computer program is efficient and rapid."}
{"id": "PMID:466761", "title": "Preservation, distribution, and assay of glucose in blood, with special reference to the newborn.", "content": "Of several preservatives we tested, a mixture of \"isotonic\" sodium fluoride and sodium iodoacetate (17.7 and 5.0 g/L, respectively) best inhibits glucose loss from blood collected from the newborn. However, the inhibition is incomplete. Twenty microliters of blood is diluted with 100 microliter of preserving solution. After centrifugation, the glucose in 100 microliter of prewarmed supernate (diluted plasma) is measured in a Beckman Glucose Analyzer, based on the action of a glucose reagent and the rate of oxygen consumption. The method, in effect, determines the glucose in whole blood, except for about 5% of the total that remains in the erythrocytes. Glucose is distributed in blood according to the water content of cells and plasma. From concentrations found in cells and plasma, we can calculate the value for glucose in whole blood with an accuracy of 95.2%. The modified method we describe shows acceptable precision, as judged from within-run and day-to-day CV--generally well below 5% for each.", "contents": "Preservation, distribution, and assay of glucose in blood, with special reference to the newborn. Of several preservatives we tested, a mixture of \"isotonic\" sodium fluoride and sodium iodoacetate (17.7 and 5.0 g/L, respectively) best inhibits glucose loss from blood collected from the newborn. However, the inhibition is incomplete. Twenty microliters of blood is diluted with 100 microliter of preserving solution. After centrifugation, the glucose in 100 microliter of prewarmed supernate (diluted plasma) is measured in a Beckman Glucose Analyzer, based on the action of a glucose reagent and the rate of oxygen consumption. The method, in effect, determines the glucose in whole blood, except for about 5% of the total that remains in the erythrocytes. Glucose is distributed in blood according to the water content of cells and plasma. From concentrations found in cells and plasma, we can calculate the value for glucose in whole blood with an accuracy of 95.2%. The modified method we describe shows acceptable precision, as judged from within-run and day-to-day CV--generally well below 5% for each."}
{"id": "PMID:466762", "title": "Further observations on the incidence and nature of atypical creatine kinase activity.", "content": "The interference of atypical creatine kinase (CK; EC 2.7.3.2) with anion-exchange methods for the measurement of the CK-MB isoenzyme is now firmly established. False-positive results from this source are much more common than interferences caused by the BB isoenzyme. Atypical CK, at least in some patients, does not appear to be a genetic variant, nor could we relate it to a specific clinical diagnosis, to hypoxia, or to administration of a particular drug. Its behavior in an immuno-inhibition test for CK-B subunit indicates an immunological difference from the normal M subunit. Thus the change in immunological properties is associated with the altered electrophoretic properties. The variability of electrophoretic patterns suggests that atypical CK may represent multiple forms of creatine kinase rather than a unique entity.", "contents": "Further observations on the incidence and nature of atypical creatine kinase activity. The interference of atypical creatine kinase (CK; EC 2.7.3.2) with anion-exchange methods for the measurement of the CK-MB isoenzyme is now firmly established. False-positive results from this source are much more common than interferences caused by the BB isoenzyme. Atypical CK, at least in some patients, does not appear to be a genetic variant, nor could we relate it to a specific clinical diagnosis, to hypoxia, or to administration of a particular drug. Its behavior in an immuno-inhibition test for CK-B subunit indicates an immunological difference from the normal M subunit. Thus the change in immunological properties is associated with the altered electrophoretic properties. The variability of electrophoretic patterns suggests that atypical CK may represent multiple forms of creatine kinase rather than a unique entity."}
{"id": "PMID:466763", "title": "Radioimmunoassay of 17 alpha-hydroxyprogesterone in saliva, parotid fluid, and plasma of congenital adrenal hyperplasia patients.", "content": "We report a radioimmunoassay sensitive enough to determine 17 alpha-hydroxyprogesterone concentrations in 200 microliter of parotid fluid or mixed whole saliva. Because the correlation of concentrations in matched samples of parotid fluid and saliva was excellent (r = 0.98), we exclusively used saliva, which is easier to collect, in later studies. The assay is specific; saliva samples assayed with and without thin-layer chromatographic purification showed no significant difference. The assay is also precise, and has a lower limit of sensitivity of 4 pg per assay tube. In 14 patients having congenital adrenal hyperplasia from a C21-hydroxylase enzyme deficiency, all of whom were receiving cortisol replacement therapy, the range in 17 alpha-hydroxyprogesterone concentrations observed in saliva (67-26,300 pmol/L) was about 20-fold that seen in 32 healthy children (90-1520 pmol/L). The close correlation (r = 0.91) between 17 alpha-hydroxyprogesterone concentrations in matched samples of saliva and plasma from these patients indicates that determination of steroids in saliva could well replace determination in plasma. This concept is supported by 17 alpha-hydroxyprogesterone concentrations monitored throughout 24 h from one patient and following stimulation with synthetic corticotropin in another patient.", "contents": "Radioimmunoassay of 17 alpha-hydroxyprogesterone in saliva, parotid fluid, and plasma of congenital adrenal hyperplasia patients. We report a radioimmunoassay sensitive enough to determine 17 alpha-hydroxyprogesterone concentrations in 200 microliter of parotid fluid or mixed whole saliva. Because the correlation of concentrations in matched samples of parotid fluid and saliva was excellent (r = 0.98), we exclusively used saliva, which is easier to collect, in later studies. The assay is specific; saliva samples assayed with and without thin-layer chromatographic purification showed no significant difference. The assay is also precise, and has a lower limit of sensitivity of 4 pg per assay tube. In 14 patients having congenital adrenal hyperplasia from a C21-hydroxylase enzyme deficiency, all of whom were receiving cortisol replacement therapy, the range in 17 alpha-hydroxyprogesterone concentrations observed in saliva (67-26,300 pmol/L) was about 20-fold that seen in 32 healthy children (90-1520 pmol/L). The close correlation (r = 0.91) between 17 alpha-hydroxyprogesterone concentrations in matched samples of saliva and plasma from these patients indicates that determination of steroids in saliva could well replace determination in plasma. This concept is supported by 17 alpha-hydroxyprogesterone concentrations monitored throughout 24 h from one patient and following stimulation with synthetic corticotropin in another patient."}
{"id": "PMID:466764", "title": "A solid-phase enzyme-linked immunosorbent assay for the quantitation of human plasma alpha 1-acid glycoprotein.", "content": "We describe a solid-phase enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in human plasma. Plasma samples are incubated with alkaline phosphatase-linked, purified alpha 1-acid glycoprotein in alpha 1-acid glycoprotein-specific antibody-coated polystyrene tubes. The alkaline phosphatase that becomes attached to the tube via an immunological reaction between the alpha 1-acid glycoprotein and the specific antibody is measured spectrophotometrically. This assay is accurate reproducible, simple, and economical. As little as 4 microgram of alpha 1-acid glycoprotein per liter can be detected. The normal range for alpha 1-acid glycoprotein in the plasma of healthy adults, as measured by this method, is 0.48-1.27 g/L; the range is significantly different, 0.29-0.73 g/L, for women who are taking oral contraceptive pills.", "contents": "A solid-phase enzyme-linked immunosorbent assay for the quantitation of human plasma alpha 1-acid glycoprotein. We describe a solid-phase enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in human plasma. Plasma samples are incubated with alkaline phosphatase-linked, purified alpha 1-acid glycoprotein in alpha 1-acid glycoprotein-specific antibody-coated polystyrene tubes. The alkaline phosphatase that becomes attached to the tube via an immunological reaction between the alpha 1-acid glycoprotein and the specific antibody is measured spectrophotometrically. This assay is accurate reproducible, simple, and economical. As little as 4 microgram of alpha 1-acid glycoprotein per liter can be detected. The normal range for alpha 1-acid glycoprotein in the plasma of healthy adults, as measured by this method, is 0.48-1.27 g/L; the range is significantly different, 0.29-0.73 g/L, for women who are taking oral contraceptive pills."}
{"id": "PMID:466765", "title": "Measurement of aspartate aminotransferase activity: effects of oxamate.", "content": "Oxamate, a potent inhibitor of lactate dehydrogenase, is shown also to inhibit aspartate aminotransferase activity, both in human serum and in purified isoenzymes of human origin. The inhibition was competitive with respect to 2-oxoglutarate for both isoenzymes. The apparent Ki was 29 mmol/L for the cytoplasmic enzyme and 17 mmol/L for the mitochondrial enzyme. Noncompetitive inhibition was found between oxamate and aspartate. At saturating concentrations of substrate (2-oxoglutarate greater than or equal to 15 mmol/L, L-aspartate greater than or equal 150 mmol/L) oxamate inhibited the mitochondrial enzyme but had less effect on the cytoplasmic isoenzyme. Oxamate at 40 mmol/L inhibited the enzyme in serum by 11 and 9% in assays containing 2-oxoglutarate at 6.7 and 15 mmol/L, respectively. This concentration of oxamate inhibited enzyme activity in serum by 5% more than did the same concentration of Cl- (itself an inhibitor). Oxamate (less than or equal to 30 mmol/L) had no measurable effect on the stability or activity of porcine malate dehydrogenase. Until the effects of its inhibitory properties are considered, addition of oxamate to suppress lactate dehydrogenase-mediated side reactions in the assay of aspartate aminotransferase cannot be recommended.", "contents": "Measurement of aspartate aminotransferase activity: effects of oxamate. Oxamate, a potent inhibitor of lactate dehydrogenase, is shown also to inhibit aspartate aminotransferase activity, both in human serum and in purified isoenzymes of human origin. The inhibition was competitive with respect to 2-oxoglutarate for both isoenzymes. The apparent Ki was 29 mmol/L for the cytoplasmic enzyme and 17 mmol/L for the mitochondrial enzyme. Noncompetitive inhibition was found between oxamate and aspartate. At saturating concentrations of substrate (2-oxoglutarate greater than or equal to 15 mmol/L, L-aspartate greater than or equal 150 mmol/L) oxamate inhibited the mitochondrial enzyme but had less effect on the cytoplasmic isoenzyme. Oxamate at 40 mmol/L inhibited the enzyme in serum by 11 and 9% in assays containing 2-oxoglutarate at 6.7 and 15 mmol/L, respectively. This concentration of oxamate inhibited enzyme activity in serum by 5% more than did the same concentration of Cl- (itself an inhibitor). Oxamate (less than or equal to 30 mmol/L) had no measurable effect on the stability or activity of porcine malate dehydrogenase. Until the effects of its inhibitory properties are considered, addition of oxamate to suppress lactate dehydrogenase-mediated side reactions in the assay of aspartate aminotransferase cannot be recommended."}
{"id": "PMID:466766", "title": "Simplified determination of urinary delta-aminolevulinic acid in a wide range of concentrations.", "content": "We describe a simple method for measuring delta-aminole-vulinic acid in urine without the need for the seldom-used but time-consuming internal-standard addition step. It combines the analytical procedure described by Tomokuni and Ogata [Clin. Chem. 18, 1534 (1972)] and a correction based on urine density to obtain, more rapidly and less expensively, results as accurate as those given by the Davis and Andelman [Arch. Environ. Health 15, 53 (1967)] chromatographic method.", "contents": "Simplified determination of urinary delta-aminolevulinic acid in a wide range of concentrations. We describe a simple method for measuring delta-aminole-vulinic acid in urine without the need for the seldom-used but time-consuming internal-standard addition step. It combines the analytical procedure described by Tomokuni and Ogata [Clin. Chem. 18, 1534 (1972)] and a correction based on urine density to obtain, more rapidly and less expensively, results as accurate as those given by the Davis and Andelman [Arch. Environ. Health 15, 53 (1967)] chromatographic method."}
{"id": "PMID:466767", "title": "Assay of 1,25-dihydroxy vitamin D3 by isotope dilution--mass fragmentography.", "content": "We describe a specific assay of 1,25-dihydroxy vitamin D3 in human serum, based on isotope dilution-mass fragmentography. We added [26-2H3]-1,25-dihydroxy vitamin D3 to a fixed amount of serum. The steroids were extracted with chloroform/methanol and purified by liquid chromatography. The purified materal was converted into the trimethylsilyl ether and analyzed. Unlabeled 1,25-dihydroxy vitamin D3 was quantitated from the ratio between the tracings at m/e 452 and 455. The two ions used correspond to loss of two trimethylsilyloxo functions from the molecular ion. Essentially the same results, with some problems of interference, were obtained when we used the more intense ions at m/e 131 and 134 (corresponding to cleavage between C-24 and C-25). The detection limit was about 5 ng/L of serum; the coefficient of variation was about 6%. The accuracy of the method was assessed by recovery experiments. 1,25-Dihydroxy vitamin D3 in sera from 15 healthy subjects was found to average 55 +/- 10 ng/L(+/- SD). We believe this represents the first determination of 1,25-dihydroxy vitamin D3 in serum by use of a method not based on radioimmunoassay or receptor assay. Slightly lower values have been reported by those latter techniques.", "contents": "Assay of 1,25-dihydroxy vitamin D3 by isotope dilution--mass fragmentography. We describe a specific assay of 1,25-dihydroxy vitamin D3 in human serum, based on isotope dilution-mass fragmentography. We added [26-2H3]-1,25-dihydroxy vitamin D3 to a fixed amount of serum. The steroids were extracted with chloroform/methanol and purified by liquid chromatography. The purified materal was converted into the trimethylsilyl ether and analyzed. Unlabeled 1,25-dihydroxy vitamin D3 was quantitated from the ratio between the tracings at m/e 452 and 455. The two ions used correspond to loss of two trimethylsilyloxo functions from the molecular ion. Essentially the same results, with some problems of interference, were obtained when we used the more intense ions at m/e 131 and 134 (corresponding to cleavage between C-24 and C-25). The detection limit was about 5 ng/L of serum; the coefficient of variation was about 6%. The accuracy of the method was assessed by recovery experiments. 1,25-Dihydroxy vitamin D3 in sera from 15 healthy subjects was found to average 55 +/- 10 ng/L(+/- SD). We believe this represents the first determination of 1,25-dihydroxy vitamin D3 in serum by use of a method not based on radioimmunoassay or receptor assay. Slightly lower values have been reported by those latter techniques."}
{"id": "PMID:466768", "title": "New fluorometric analysis for mandelic and phenylglyoxylic acids in urine as an index to styrene exposure.", "content": "I describe a new fluorometric method for determination of mandelic and phenylglyoxylic acids in urine, the fluorometry being preceded by extraction into ether and thin-layer chromatography. The chromatographically separated acids were quantitated after conversion to stable highly fluorescent derivatives by treatment with concentrated sulfuric acid. This method proves to be more precise, accurate, and reproducible than the existing colorimetric method. The limit of detection is 2 microgram of either acid per milliliter of urine with a CV of less than 15%. The standard curve for either acid is essentially linear from 2 to 100 microgram/mL of urine, with a correlation coefficient (r) of 0.97. No satisfactory correlation was obtained between the concentrations of either acid metabolite as measured in rat urine by fluorometry and colorimetry. The present method is considered suitable for routine biological monitoring of persons exposed to both high and low concentrations of styrene.", "contents": "New fluorometric analysis for mandelic and phenylglyoxylic acids in urine as an index to styrene exposure. I describe a new fluorometric method for determination of mandelic and phenylglyoxylic acids in urine, the fluorometry being preceded by extraction into ether and thin-layer chromatography. The chromatographically separated acids were quantitated after conversion to stable highly fluorescent derivatives by treatment with concentrated sulfuric acid. This method proves to be more precise, accurate, and reproducible than the existing colorimetric method. The limit of detection is 2 microgram of either acid per milliliter of urine with a CV of less than 15%. The standard curve for either acid is essentially linear from 2 to 100 microgram/mL of urine, with a correlation coefficient (r) of 0.97. No satisfactory correlation was obtained between the concentrations of either acid metabolite as measured in rat urine by fluorometry and colorimetry. The present method is considered suitable for routine biological monitoring of persons exposed to both high and low concentrations of styrene."}
{"id": "PMID:466769", "title": "Separation of protoporphyrins and related compounds by reversed-phase liquid chromatography.", "content": "We describe the separation of protoporphyrin and related porphyrins by reversed-phase \"high-performance\" liquid chromatography, with fluorometric detection. We used the method to demonstrate that acid hydrolysis of the dimethyl ester of protoporphyrin IX is complete in 2 to 3 h and is followed by the acid-catalyzed conversion of protoporphyrin IX to a chemical species that chromatographic evidence indicates to be hematoporphyrin IX. In addition, the method was used to evaluate the purity of a commercial preparation of protoporphyrin IX and was also demonstrated to have the sensitivity and specificity needed for measuring the protoporphyrin IX content of whole-blood.", "contents": "Separation of protoporphyrins and related compounds by reversed-phase liquid chromatography. We describe the separation of protoporphyrin and related porphyrins by reversed-phase \"high-performance\" liquid chromatography, with fluorometric detection. We used the method to demonstrate that acid hydrolysis of the dimethyl ester of protoporphyrin IX is complete in 2 to 3 h and is followed by the acid-catalyzed conversion of protoporphyrin IX to a chemical species that chromatographic evidence indicates to be hematoporphyrin IX. In addition, the method was used to evaluate the purity of a commercial preparation of protoporphyrin IX and was also demonstrated to have the sensitivity and specificity needed for measuring the protoporphyrin IX content of whole-blood."}
{"id": "PMID:466770", "title": "Improved automated kinetic determination of uric acid in serum by use of uricase/catalase/aldehyde dehydrogenase.", "content": "The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydrogenase isoenzymes. Among other substances, a mixture of pyrazole and oxalate can be used to eliminate these interferences. This inhibitor system gives good results when used in the automated kinetic uric acid determination, as is shown by a comparison with the manual assay for uric acid according to Kageyama [Clin. Chim. Acta 31, 421 (1971)].", "contents": "Improved automated kinetic determination of uric acid in serum by use of uricase/catalase/aldehyde dehydrogenase. The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydrogenase isoenzymes. Among other substances, a mixture of pyrazole and oxalate can be used to eliminate these interferences. This inhibitor system gives good results when used in the automated kinetic uric acid determination, as is shown by a comparison with the manual assay for uric acid according to Kageyama [Clin. Chim. Acta 31, 421 (1971)]."}
{"id": "PMID:466771", "title": "Preparation of cytosol for steroid receptor determinations with the Beckman \"Airfuge\".", "content": "Preparation of cytosols from several estrogen target organs was compared, with use of the Beckman Airfuge and a preparative ultracentrifuge. The specific estrogen-binding capacities of cytosols prepared with these instruments were indistinguishable. Similarly, the sedimentation profiles of estrogen-receptor species as determined by sucrose gradient centrifugation were identical. We conclude that the Airfuge provides a rapid, accurate, and inexpensive means of preparing cytosols for measurements of steroid receptors in the clinical laboratory.", "contents": "Preparation of cytosol for steroid receptor determinations with the Beckman \"Airfuge\". Preparation of cytosols from several estrogen target organs was compared, with use of the Beckman Airfuge and a preparative ultracentrifuge. The specific estrogen-binding capacities of cytosols prepared with these instruments were indistinguishable. Similarly, the sedimentation profiles of estrogen-receptor species as determined by sucrose gradient centrifugation were identical. We conclude that the Airfuge provides a rapid, accurate, and inexpensive means of preparing cytosols for measurements of steroid receptors in the clinical laboratory."}
{"id": "PMID:466772", "title": "Effect of light and temperature on the stability of creatine kinase in human sera and controls.", "content": "Commercial control preparations were exposed to light at room temperature for as long as 24 h. Decreases in creatine kinase activity in seven of the nine controls ranged from 28.8 to 83.3%. When these controls were protected from light and stored at various temperatures, the decrease in activity was either eliminated or substantially reduced, indicating that the decreased activity in the presence of light was not due entirely to thermal inactivation. In the absence of oxygen, light had little effect on creatine kinase suggesting that light inactivation is a light-catalyzed oxidative process. The decreased activity observed when some of the controls were exposed to light was not completely restored by incubating with dithiothreitol before analysis. However, increases in creatine kinase activity ranging from about 37 to 176% were observed when freshly prepared controls were pre-incubated with dithiothreitol. Neither light nor dithiothreitol had any effect on the creatine kinase activity in two of the nine controls. When human sera were exposed to light for 24 h, the largest decrease in activity was about 15%. Incubation of fresh human sera with dithiothreitol before analysis caused an average increase in activity of approximately 10%.", "contents": "Effect of light and temperature on the stability of creatine kinase in human sera and controls. Commercial control preparations were exposed to light at room temperature for as long as 24 h. Decreases in creatine kinase activity in seven of the nine controls ranged from 28.8 to 83.3%. When these controls were protected from light and stored at various temperatures, the decrease in activity was either eliminated or substantially reduced, indicating that the decreased activity in the presence of light was not due entirely to thermal inactivation. In the absence of oxygen, light had little effect on creatine kinase suggesting that light inactivation is a light-catalyzed oxidative process. The decreased activity observed when some of the controls were exposed to light was not completely restored by incubating with dithiothreitol before analysis. However, increases in creatine kinase activity ranging from about 37 to 176% were observed when freshly prepared controls were pre-incubated with dithiothreitol. Neither light nor dithiothreitol had any effect on the creatine kinase activity in two of the nine controls. When human sera were exposed to light for 24 h, the largest decrease in activity was about 15%. Incubation of fresh human sera with dithiothreitol before analysis caused an average increase in activity of approximately 10%."}
{"id": "PMID:466781", "title": "Potential clinical applications of derivative and wavelength-modulation spectrometry.", "content": "Absorption spectrophotometers are now becoming available that can record the first and second derivatives of absorbance with respect to wavelength. The advantages of this technique and its potential application to clinical analysis are discussed.", "contents": "Potential clinical applications of derivative and wavelength-modulation spectrometry. Absorption spectrophotometers are now becoming available that can record the first and second derivatives of absorbance with respect to wavelength. The advantages of this technique and its potential application to clinical analysis are discussed."}
{"id": "PMID:466782", "title": "Microencapsulated antibodies in radioimmunoassay. II. Determination of free thyroxine.", "content": "We describe a method for directly measuring free thyroxine in human serum. Antibody to thyroxine was encapsulated within a semipermeable nylon membrane that excludes substances of relative molecular mass greater than 20,000. The microencapsulated antibody was then presaturated with [125I]thyroxine. Serum incubated with the microcapsules initiated a displacement reaction between free thyroxine and [125I]thyroxine bound to the antibody. The displaced thyroxine was separated from the bound thyroxine by centrifugation and the concentration of free thyroxine determined from a standard curve prepared by use of known amounts of free thyroxine. The within-day coefficient of variation for two control samples was 8.8 and 6.8%; day-to-day precision for the same material was 12.5 and 13.5%. Lipemia, icterus, or hemoglobin had no adverse effect. Interlaboratory evaluation of the procedure revealed no significant difference in mean values for 20 speciments (p greater than 0.05).", "contents": "Microencapsulated antibodies in radioimmunoassay. II. Determination of free thyroxine. We describe a method for directly measuring free thyroxine in human serum. Antibody to thyroxine was encapsulated within a semipermeable nylon membrane that excludes substances of relative molecular mass greater than 20,000. The microencapsulated antibody was then presaturated with [125I]thyroxine. Serum incubated with the microcapsules initiated a displacement reaction between free thyroxine and [125I]thyroxine bound to the antibody. The displaced thyroxine was separated from the bound thyroxine by centrifugation and the concentration of free thyroxine determined from a standard curve prepared by use of known amounts of free thyroxine. The within-day coefficient of variation for two control samples was 8.8 and 6.8%; day-to-day precision for the same material was 12.5 and 13.5%. Lipemia, icterus, or hemoglobin had no adverse effect. Interlaboratory evaluation of the procedure revealed no significant difference in mean values for 20 speciments (p greater than 0.05)."}
{"id": "PMID:466783", "title": "Plasma polyamines determined by negative-ion chemical ionization/mass spectrometry.", "content": "We have developed an accurate and highly sensitive gas-chromatographic/mass-spectrometric procedure for determining di- and polyamines (putrescine, spermidine, and spermine) in plasma and erythrocytes. Deuterium-labeled analogs of putrescine, spermidine, and spermine were synthesized for use as internal standards. Trifluoroacetyl derivatives of the polyamines were formed during the procedure and then detected with negative-ion chemical ionization/mass spectrometry in combination with multiple ion monitoring. Limits of sensitivity ranged from 0.25 to 1.0 pmol of analyte injected into the instrument.", "contents": "Plasma polyamines determined by negative-ion chemical ionization/mass spectrometry. We have developed an accurate and highly sensitive gas-chromatographic/mass-spectrometric procedure for determining di- and polyamines (putrescine, spermidine, and spermine) in plasma and erythrocytes. Deuterium-labeled analogs of putrescine, spermidine, and spermine were synthesized for use as internal standards. Trifluoroacetyl derivatives of the polyamines were formed during the procedure and then detected with negative-ion chemical ionization/mass spectrometry in combination with multiple ion monitoring. Limits of sensitivity ranged from 0.25 to 1.0 pmol of analyte injected into the instrument."}
{"id": "PMID:466784", "title": "A kinetic method for glucose that is insensitive to variations in temperature and enzyme activity.", "content": "We have adapted for glucose determination a new approach to kinetic analyses [Anal. Chem. 50, 1611 (1978)]; it is 50-fold less dependent upon some experimental variables than is a more conventional rate method. Modification of a commercially available hexokinase/glucose-6-phosphate dehydrogenase reagent system for glucose provides that the rate of production of NADH be first-order in total glucose concentration within about 30 s after sample and reagent are mixed. In the kinetic method, absorbance vs. time data recorded after 30 s and a multiple-linear-regression program are used to compute the absorbance change that would occur if the reaction were monitored to completion. Results demonstrate a linear relationship between glucose concentration and computed absorbance change. Application of the method to 51 human sera without rigorous control of either temperature or reagent composition yielded a regression equation of y = 1.01x -0.3 when kinetic results (y) were compared with equilibrium results (x) for the same samples analyzed in a hospital laboratory.", "contents": "A kinetic method for glucose that is insensitive to variations in temperature and enzyme activity. We have adapted for glucose determination a new approach to kinetic analyses [Anal. Chem. 50, 1611 (1978)]; it is 50-fold less dependent upon some experimental variables than is a more conventional rate method. Modification of a commercially available hexokinase/glucose-6-phosphate dehydrogenase reagent system for glucose provides that the rate of production of NADH be first-order in total glucose concentration within about 30 s after sample and reagent are mixed. In the kinetic method, absorbance vs. time data recorded after 30 s and a multiple-linear-regression program are used to compute the absorbance change that would occur if the reaction were monitored to completion. Results demonstrate a linear relationship between glucose concentration and computed absorbance change. Application of the method to 51 human sera without rigorous control of either temperature or reagent composition yielded a regression equation of y = 1.01x -0.3 when kinetic results (y) were compared with equilibrium results (x) for the same samples analyzed in a hospital laboratory."}
{"id": "PMID:466785", "title": "New developments in analysis of isoenzymes separated by \"high-performance\" liquid chromatography.", "content": "We have developed two enzyme analyzers for use in \"high-performance\" liquid chromatography. In both systems two detectors are used, placed after the column effluent has been combined with assay reagent. In one system, an absorbance detector is placed before and after a post-column reaction coil. Peaks observed at one detector are subtracted from those at the other, to produce a two-point measurement of enzyme activity. The linear dynamic range was 17--1700 U/L for lactate dehydrogenase (EC 1.1.1.27). In the other system, two reaction coils were used and a single fluorescence detector was placed at the end of each coil. These coils were kept at different temperatures, and an automated switching valve diverted equal amounts of column effluent and reagent into both coils. The fluorescence readings were then subtracted to produce a differential measurement of enzyme activity. The linear dynamic range was 20--1000 U/L. We used both systems to chromatographically analyze lactate dehydrogenase isoenzymes, and could separately determine both the distribution and activity of sample isoenzymes.", "contents": "New developments in analysis of isoenzymes separated by \"high-performance\" liquid chromatography. We have developed two enzyme analyzers for use in \"high-performance\" liquid chromatography. In both systems two detectors are used, placed after the column effluent has been combined with assay reagent. In one system, an absorbance detector is placed before and after a post-column reaction coil. Peaks observed at one detector are subtracted from those at the other, to produce a two-point measurement of enzyme activity. The linear dynamic range was 17--1700 U/L for lactate dehydrogenase (EC 1.1.1.27). In the other system, two reaction coils were used and a single fluorescence detector was placed at the end of each coil. These coils were kept at different temperatures, and an automated switching valve diverted equal amounts of column effluent and reagent into both coils. The fluorescence readings were then subtracted to produce a differential measurement of enzyme activity. The linear dynamic range was 20--1000 U/L. We used both systems to chromatographically analyze lactate dehydrogenase isoenzymes, and could separately determine both the distribution and activity of sample isoenzymes."}
{"id": "PMID:466786", "title": "Rapid analysis of bilirubin in neonatal serum. I. The binding of bilirubin to albumin.", "content": "Evaulation of the severity of jaundice in the neonate may be determined by measuring the reserve binding capacity of serum proteins for free bilirubin. Determination of protein-bound bilirubin has been labor intensive, necessitating multiple runs on gel-permeation chromatography columns or, more recently, enzyme assays or fluorescence quenching analysis. We present a method for quantitation of free bilirubin and of bilirubin-binding capacity of serum by liquid chromatography. A gel-permeation column binds free bilirubin while allowing passage and quantitation of protein-bound bilirubin. Subsequent injection of a desorbing agent releases the adsorbed bilirubin from the column, permitting quantitation of free bilirubin. Bound and free serum bilirubin may be determined directly in less than 15 min using 10 microL of serum. The binding of bilirubin to neonatal serum is seen to be quite different from the binding to adult serum. Ion-exchange chromatography of adult and neonatal serum samples shows that their protein profiles are radically different. This difference probably accounts for the binding characteristics.", "contents": "Rapid analysis of bilirubin in neonatal serum. I. The binding of bilirubin to albumin. Evaulation of the severity of jaundice in the neonate may be determined by measuring the reserve binding capacity of serum proteins for free bilirubin. Determination of protein-bound bilirubin has been labor intensive, necessitating multiple runs on gel-permeation chromatography columns or, more recently, enzyme assays or fluorescence quenching analysis. We present a method for quantitation of free bilirubin and of bilirubin-binding capacity of serum by liquid chromatography. A gel-permeation column binds free bilirubin while allowing passage and quantitation of protein-bound bilirubin. Subsequent injection of a desorbing agent releases the adsorbed bilirubin from the column, permitting quantitation of free bilirubin. Bound and free serum bilirubin may be determined directly in less than 15 min using 10 microL of serum. The binding of bilirubin to neonatal serum is seen to be quite different from the binding to adult serum. Ion-exchange chromatography of adult and neonatal serum samples shows that their protein profiles are radically different. This difference probably accounts for the binding characteristics."}
{"id": "PMID:466787", "title": "Improved method for detecting hemagglutination by centrifugal analysis.", "content": "Centrifugal analysis can be used to detect hepatitis B surface antigen, antibody to rubella virus, and fibrin-related antigen. The procedure is performed with the same reagents used in conventional hemagglutination studies. Positive and negative reactions are distinguished by the rates of erythrocyte clearance in the centrifugal field (delta A/delta time); positive cells move more rapidly than negative cells, and this difference varies directly with the concentration of detectable antigen or antibody. This phenomenon is thought to be a result of the greater adhesion of negative cells to the cuvette's surface. Sensitivity and specificity are greater in the centrifugal analysis technique than in the more conventional hemagglutination tests. False-positive reactions are eliminated and the quantitative data are accurate and reproducible.", "contents": "Improved method for detecting hemagglutination by centrifugal analysis. Centrifugal analysis can be used to detect hepatitis B surface antigen, antibody to rubella virus, and fibrin-related antigen. The procedure is performed with the same reagents used in conventional hemagglutination studies. Positive and negative reactions are distinguished by the rates of erythrocyte clearance in the centrifugal field (delta A/delta time); positive cells move more rapidly than negative cells, and this difference varies directly with the concentration of detectable antigen or antibody. This phenomenon is thought to be a result of the greater adhesion of negative cells to the cuvette's surface. Sensitivity and specificity are greater in the centrifugal analysis technique than in the more conventional hemagglutination tests. False-positive reactions are eliminated and the quantitative data are accurate and reproducible."}
{"id": "PMID:466788", "title": "High-performance liquid chromatography for ultraviolet-absorbing constituents in human urine.", "content": "Several liquid-chromatographic systems involving anion-exchange resins and linear acetate gradient elution have been reported previously for the analysis of ultraviolet-absorbing constituents in human urine. We describe a high-performance liquid-chromatographic system on which a macroreticular anion-exchange resin, and stepwise elution are used. Separations were more rapid with ammonium chloride/acetonitrile stepwise elution than with acetate stepwise elution, and urine constituents could be detected at 225 nm with the former system. Analytical conditions were convenient and the method has proven to be useful for routine clinical analysis.", "contents": "High-performance liquid chromatography for ultraviolet-absorbing constituents in human urine. Several liquid-chromatographic systems involving anion-exchange resins and linear acetate gradient elution have been reported previously for the analysis of ultraviolet-absorbing constituents in human urine. We describe a high-performance liquid-chromatographic system on which a macroreticular anion-exchange resin, and stepwise elution are used. Separations were more rapid with ammonium chloride/acetonitrile stepwise elution than with acetate stepwise elution, and urine constituents could be detected at 225 nm with the former system. Analytical conditions were convenient and the method has proven to be useful for routine clinical analysis."}
{"id": "PMID:466789", "title": "Instrumentation and techniques for analysis of hydrogen peroxide and peroxide-producing reactions involving earthworm (Diplocardia longa) bioluminescence.", "content": "Earthworm bioluminescence (species Diplocardia longa) is a sensitive tool for determination of peroxide titers in biological systems, with linearity of the standard curve for hydrogen peroxide extending from 10 nmol/L in the assay to greater than 1 mmol/L, and with sensitivity to 2 pmol of peroxide per assay. This assay's insensitivity to extraneous protein, turbidity, and solutes that are not redox active makes it particularly useful for automated or crude sample analysis and for analysis of oxidases and their substrates in coupled reactions. Examples of this latter application we discuss are analyses of glucose and putrescine oxidases and their substrates. In each case linear standard curves are obtained, covering several decades of concentration. The coupled reactions also allow in situ detection of oxidases in electrophoresis gels. We also describe a small, portable photometer system and an on-line computer-controlled instrument system that has facilitated development of these assays and will also allow automatic scanning of bioluminescence in gels. The data-collection and decision-making algorithms of this system are simple and easily adaptable for use in an inexpensive computer-based photometer system.", "contents": "Instrumentation and techniques for analysis of hydrogen peroxide and peroxide-producing reactions involving earthworm (Diplocardia longa) bioluminescence. Earthworm bioluminescence (species Diplocardia longa) is a sensitive tool for determination of peroxide titers in biological systems, with linearity of the standard curve for hydrogen peroxide extending from 10 nmol/L in the assay to greater than 1 mmol/L, and with sensitivity to 2 pmol of peroxide per assay. This assay's insensitivity to extraneous protein, turbidity, and solutes that are not redox active makes it particularly useful for automated or crude sample analysis and for analysis of oxidases and their substrates in coupled reactions. Examples of this latter application we discuss are analyses of glucose and putrescine oxidases and their substrates. In each case linear standard curves are obtained, covering several decades of concentration. The coupled reactions also allow in situ detection of oxidases in electrophoresis gels. We also describe a small, portable photometer system and an on-line computer-controlled instrument system that has facilitated development of these assays and will also allow automatic scanning of bioluminescence in gels. The data-collection and decision-making algorithms of this system are simple and easily adaptable for use in an inexpensive computer-based photometer system."}
{"id": "PMID:466790", "title": "Flow-injection analysis with chemiluminescence detection.", "content": "In \"flow-injection analysis,\" the sample is inserted into a stream of reagent by use of a sample-injection valve. Mixing occurs downstream from the valve in a coil of tubing. With chemiluminescence detection this coil is positioned in front of a photomultiplier. We have evaluated the system for detection of hydrogen peroxide, using the luminol reaction with cupric ion as a catalyst. The effects of flow rate, sample volume, and reaction kinetics on the magnitude, duration, and repeatability of the chemiluminescent response have been evaluated. Precisions of 1 to 2% relative standard deviation on replicate measurements are readily achievable. A sample throughput of six samples per minute is possible with very little peak overlap. This detection system can be coupled to any process in which peroxide is generated.", "contents": "Flow-injection analysis with chemiluminescence detection. In \"flow-injection analysis,\" the sample is inserted into a stream of reagent by use of a sample-injection valve. Mixing occurs downstream from the valve in a coil of tubing. With chemiluminescence detection this coil is positioned in front of a photomultiplier. We have evaluated the system for detection of hydrogen peroxide, using the luminol reaction with cupric ion as a catalyst. The effects of flow rate, sample volume, and reaction kinetics on the magnitude, duration, and repeatability of the chemiluminescent response have been evaluated. Precisions of 1 to 2% relative standard deviation on replicate measurements are readily achievable. A sample throughput of six samples per minute is possible with very little peak overlap. This detection system can be coupled to any process in which peroxide is generated."}
{"id": "PMID:466791", "title": "Bioluminescence assay of creatine kinase and its isoenzymes in serum and cerebrospinal fluid.", "content": "We examined the sensitivity of bioluminescence for the determination of very low concentrations of creatine kinase brain-type subunit (CK-BB) in serum and in cerebrospinal fluid. To optimize the sensitivity of CK-isoenzyme assays and eliminate possible sources of error, we separated the isoenzyme fractions by using inhibiting anti-MM and precipitating anti-MM and anti-BB antibodies. The results with the bioluminescence assay correlated with spectrophotometric values such that r = 0.97 for the total CK activity and r = 0.98 for the CK-B activity. The reproducibility of the present method was comparable with the spectrophotometric method and was even better at low enzyme activities. The within-series precision for assay of total CK activity at 2 U/L corresponded to a CV of 9%; at 13 U/L the CV was 5.8%. All the assays were carried out at 25 degrees C. Even at this low temperature, CK activities as low as 0.2 U/L could be determined. In eight patients without any evidence of cerebral cell damage, total CK activity in cerebrospinal fluid was x = 1.05 +/- 0.6 U/L, and CK-BB activity was x = 0.7 +/- 0.4 U/L. In sera of these patients CK-BB activity was x = 0.6 +/- 0.5 U/L. Differences in CK and CK-BB activities in four patients with transient or progressive brain-cell damage are discussed.", "contents": "Bioluminescence assay of creatine kinase and its isoenzymes in serum and cerebrospinal fluid. We examined the sensitivity of bioluminescence for the determination of very low concentrations of creatine kinase brain-type subunit (CK-BB) in serum and in cerebrospinal fluid. To optimize the sensitivity of CK-isoenzyme assays and eliminate possible sources of error, we separated the isoenzyme fractions by using inhibiting anti-MM and precipitating anti-MM and anti-BB antibodies. The results with the bioluminescence assay correlated with spectrophotometric values such that r = 0.97 for the total CK activity and r = 0.98 for the CK-B activity. The reproducibility of the present method was comparable with the spectrophotometric method and was even better at low enzyme activities. The within-series precision for assay of total CK activity at 2 U/L corresponded to a CV of 9%; at 13 U/L the CV was 5.8%. All the assays were carried out at 25 degrees C. Even at this low temperature, CK activities as low as 0.2 U/L could be determined. In eight patients without any evidence of cerebral cell damage, total CK activity in cerebrospinal fluid was x = 1.05 +/- 0.6 U/L, and CK-BB activity was x = 0.7 +/- 0.4 U/L. In sera of these patients CK-BB activity was x = 0.6 +/- 0.5 U/L. Differences in CK and CK-BB activities in four patients with transient or progressive brain-cell damage are discussed."}
{"id": "PMID:466792", "title": "A new screening system for nonsteroidal anti-inflammatory drugs based upon inhibition of chemiluminescence produced from human cells (granulocytes).", "content": "A new screening system for nonsteroidal anti-inflammatory drugs that involves use of human phagocytic cells has been developed in which chemiluminescence measurement is used. Luminol-dependent chemiluminescence is measured after the addition of opsonized (coated with antibodies and complement) zymosan particles to human granulocytic leukocytes in the presence or absence of drugs. Of all the compounds tested, indomethacin was the most potent in blocking chemiluminescence, with measurable inhibitory activity at 5 mumol/L. The order of inhibitory potency at 0.1 mmol/L and in the presence of Ca2+ and Mg2+ was indomethacin greater than sodium salicylate greater than fenoprofen Ca greater than tolmetin greater than naproxen greater than ibuprofen. It is likely that the active compound itself must be added to the system because aspirin did not inhibit chemiluminescence, whereas its metabolite, sodium salicylate, was markedly inhibitory. Dexamethasone and methylprednisolone also did not inhibit chemiluminescence. The drugs that inhibit chemiluminescence are also known inhibitors of prostaglandin synthase (cyclooxygenase portion).", "contents": "A new screening system for nonsteroidal anti-inflammatory drugs based upon inhibition of chemiluminescence produced from human cells (granulocytes). A new screening system for nonsteroidal anti-inflammatory drugs that involves use of human phagocytic cells has been developed in which chemiluminescence measurement is used. Luminol-dependent chemiluminescence is measured after the addition of opsonized (coated with antibodies and complement) zymosan particles to human granulocytic leukocytes in the presence or absence of drugs. Of all the compounds tested, indomethacin was the most potent in blocking chemiluminescence, with measurable inhibitory activity at 5 mumol/L. The order of inhibitory potency at 0.1 mmol/L and in the presence of Ca2+ and Mg2+ was indomethacin greater than sodium salicylate greater than fenoprofen Ca greater than tolmetin greater than naproxen greater than ibuprofen. It is likely that the active compound itself must be added to the system because aspirin did not inhibit chemiluminescence, whereas its metabolite, sodium salicylate, was markedly inhibitory. Dexamethasone and methylprednisolone also did not inhibit chemiluminescence. The drugs that inhibit chemiluminescence are also known inhibitors of prostaglandin synthase (cyclooxygenase portion)."}
{"id": "PMID:466808", "title": "Sulfonamide inhibition of human alkaline phosphatase.", "content": "Several sulfonamides used as antibacterial or diuretic drugs are potent alkaline phosphatase inhibitors. The mechanism of inhibition may involve binding of the zinc in the active site of the enzyme by the unbonded electron pair on the sulfonamide group nitrogen atom as well as binding of the drug to a second site. Addition of progressively larger groups to this nitrogen leads to an increasing loss of inhibitory capability. Isoenzymes from human liver, bone, kidney, granulation tissue and intestine are inhibited to a similar extent while the placenta isoenzyme is more resistant. It is suggested that some pharmacologic actions of sulfonamides may be due to inhibition of alkaline phosphatase, rather than carbonic anhydrase.", "contents": "Sulfonamide inhibition of human alkaline phosphatase. Several sulfonamides used as antibacterial or diuretic drugs are potent alkaline phosphatase inhibitors. The mechanism of inhibition may involve binding of the zinc in the active site of the enzyme by the unbonded electron pair on the sulfonamide group nitrogen atom as well as binding of the drug to a second site. Addition of progressively larger groups to this nitrogen leads to an increasing loss of inhibitory capability. Isoenzymes from human liver, bone, kidney, granulation tissue and intestine are inhibited to a similar extent while the placenta isoenzyme is more resistant. It is suggested that some pharmacologic actions of sulfonamides may be due to inhibition of alkaline phosphatase, rather than carbonic anhydrase."}
{"id": "PMID:466809", "title": "Gelatin derivatives in blood: interference with the determination of inorganic phosphate in serum.", "content": "The method of Goldenberg and Fernandez (1966) Clin. Chem. 12, 871--882) for the determination of inorganic phosphate in serum is significantly influenced by turbidity after intravenous application of gelatin derivatives, even at low quantities. This turbidity can be eliminated by the addition of sodium dodecyl sulphate to the reaction solution.", "contents": "Gelatin derivatives in blood: interference with the determination of inorganic phosphate in serum. The method of Goldenberg and Fernandez (1966) Clin. Chem. 12, 871--882) for the determination of inorganic phosphate in serum is significantly influenced by turbidity after intravenous application of gelatin derivatives, even at low quantities. This turbidity can be eliminated by the addition of sodium dodecyl sulphate to the reaction solution."}
{"id": "PMID:466810", "title": "The occurrence of gamma-glutamylphenylalanine in the urine of newborn phenylketonurics.", "content": "The urine of untreated phenylketonurics in the first weeks of life contains gamma-glutamylphenylalanine (0.07--0.69 mmol/g creatinine, 12 samples) visible on the normal 2-dimensional electrophoreto-chromatogram. This compound is less prominent or absent when urine from older untreated phenylketonurics is examined and is not seen in normal urine.", "contents": "The occurrence of gamma-glutamylphenylalanine in the urine of newborn phenylketonurics. The urine of untreated phenylketonurics in the first weeks of life contains gamma-glutamylphenylalanine (0.07--0.69 mmol/g creatinine, 12 samples) visible on the normal 2-dimensional electrophoreto-chromatogram. This compound is less prominent or absent when urine from older untreated phenylketonurics is examined and is not seen in normal urine."}
{"id": "PMID:466811", "title": "The rapid extraction of paraquat from plasma using an ion-pairing technique.", "content": "A method for the quantitative extraction of paraquat from plasma is described. The method relies on the use of an organic-soluble ion pair of paraquat with dodecyl sulphate. The use of the extraction procedure in conjunction with the dithionite colour reaction gives a method which is suitable for emergency estimations with a sensitivity limit of 50 micrograms/l. The extraction procedure alone offers a new first step in the clean up of paraquat for analysis by other methods.", "contents": "The rapid extraction of paraquat from plasma using an ion-pairing technique. A method for the quantitative extraction of paraquat from plasma is described. The method relies on the use of an organic-soluble ion pair of paraquat with dodecyl sulphate. The use of the extraction procedure in conjunction with the dithionite colour reaction gives a method which is suitable for emergency estimations with a sensitivity limit of 50 micrograms/l. The extraction procedure alone offers a new first step in the clean up of paraquat for analysis by other methods."}
{"id": "PMID:466812", "title": "Emergency estimations of paraquat in plasma. A comparison of the RIA and ion pair/colorimetric methods.", "content": "A comparison was made between the RIA method for paraquat in plasma, and a modified colorimetric method involving ion-pair extraction of paraquat. The comparison was carried out on plasma from patients admitted with paraquat poisoning. The correlation between the methods was good and both were found to be sufficiently rapid for emergency use.", "contents": "Emergency estimations of paraquat in plasma. A comparison of the RIA and ion pair/colorimetric methods. A comparison was made between the RIA method for paraquat in plasma, and a modified colorimetric method involving ion-pair extraction of paraquat. The comparison was carried out on plasma from patients admitted with paraquat poisoning. The correlation between the methods was good and both were found to be sufficiently rapid for emergency use."}
{"id": "PMID:466813", "title": "Osmotic stability of erythrocytes in human muscular dystrophy before and after phospholipase treatment.", "content": "The stability of washed erythrocytes from patients with muscular dystrophy was determined in hypotonic phosphate buffered sodium chloride. Control cells were more stable than cells from Duchenne and myotonic patients. After pretreatment of the cells with phospholipase from pancreas, snake venom or bee venom in the presence of 14 mmol/l Ca2+, the order of osmotic stability in the 3 groups was not changed. In isotonic phosphate buffered NaCl, however, the erythrocytes of the myotonic patients were much more stable than the cells of the Duchenne and the control group. The lytic process was further studied in control cells with pancreatic phospholipase. 21 +/- 3 (S.E.M.) % of the cells were lysed. This process was (partly) prevented by omitting the phospholipase, by replacement of Na+ by K+ or Li+, by lowering the Ca2+ concentration, by omitting phosphate, by ouabain, by glucose, by ribose, by sucrose, by tetrodotoxin, a Na+-transport inhibitor. Blocking of the Ca2+ transport by La3+ or mersalyl, greatly stimulated the lytic process.", "contents": "Osmotic stability of erythrocytes in human muscular dystrophy before and after phospholipase treatment. The stability of washed erythrocytes from patients with muscular dystrophy was determined in hypotonic phosphate buffered sodium chloride. Control cells were more stable than cells from Duchenne and myotonic patients. After pretreatment of the cells with phospholipase from pancreas, snake venom or bee venom in the presence of 14 mmol/l Ca2+, the order of osmotic stability in the 3 groups was not changed. In isotonic phosphate buffered NaCl, however, the erythrocytes of the myotonic patients were much more stable than the cells of the Duchenne and the control group. The lytic process was further studied in control cells with pancreatic phospholipase. 21 +/- 3 (S.E.M.) % of the cells were lysed. This process was (partly) prevented by omitting the phospholipase, by replacement of Na+ by K+ or Li+, by lowering the Ca2+ concentration, by omitting phosphate, by ouabain, by glucose, by ribose, by sucrose, by tetrodotoxin, a Na+-transport inhibitor. Blocking of the Ca2+ transport by La3+ or mersalyl, greatly stimulated the lytic process."}
{"id": "PMID:466814", "title": "Serum tetraiodothyroacetate (T4A) levels in normal healthy euthyroid individuals determined by gas chromatography-mass fragmentography (GC-MF).", "content": "A gas chromatography-mass fragmentography (GC-MF) assay for T4A in human serum is described. The assay involves a multistage extraction, followed by conversion of T4A to its dimethyl derivative prior to GC-MF. Using this assay, the concentration of T4A in 37 normal euthyroid subjects was, mean +/- S.D., 114.5 +/- 26.4 pmol/l, which is considerably lower than previous reports. Thyroxine binding prealbumin (TBPA) did not appear to be a major determinant of T4A concentration.", "contents": "Serum tetraiodothyroacetate (T4A) levels in normal healthy euthyroid individuals determined by gas chromatography-mass fragmentography (GC-MF). A gas chromatography-mass fragmentography (GC-MF) assay for T4A in human serum is described. The assay involves a multistage extraction, followed by conversion of T4A to its dimethyl derivative prior to GC-MF. Using this assay, the concentration of T4A in 37 normal euthyroid subjects was, mean +/- S.D., 114.5 +/- 26.4 pmol/l, which is considerably lower than previous reports. Thyroxine binding prealbumin (TBPA) did not appear to be a major determinant of T4A concentration."}
{"id": "PMID:466815", "title": "Determination of volatile fatty acids in urine by gas chromatography on a new stationary phase.", "content": "A rapid gas chromatographic method for determination of volatile fatty acids in urine is described. Acids up to five carbon atoms, including their isomers can be separated in less than six minutes on the new stationary phase Fluorad FC430. Good separation and sensitivity is obtained with isothermal conditions.", "contents": "Determination of volatile fatty acids in urine by gas chromatography on a new stationary phase. A rapid gas chromatographic method for determination of volatile fatty acids in urine is described. Acids up to five carbon atoms, including their isomers can be separated in less than six minutes on the new stationary phase Fluorad FC430. Good separation and sensitivity is obtained with isothermal conditions."}
{"id": "PMID:466816", "title": "Catalase, superoxide dismutase, glutathione reductase and thiobarbituric acid-reactive products in normal and dystrophic human muscle.", "content": "The level of thiobarbituric acid-reactive products and the specific activities of catalase and glutathione reductase were significantly higher in muscles from patients with major forms of muscular dystrophies over those of control subjects. Superoxide dismutase activity was not altered in dystrophic muscles. These findings indicate the occurrence in human dystrophic muscles of an increased level of lipid peroxidation and the possible activation of certain enzymes that could conceivably inhibit lipid peroxidation in vivo.", "contents": "Catalase, superoxide dismutase, glutathione reductase and thiobarbituric acid-reactive products in normal and dystrophic human muscle. The level of thiobarbituric acid-reactive products and the specific activities of catalase and glutathione reductase were significantly higher in muscles from patients with major forms of muscular dystrophies over those of control subjects. Superoxide dismutase activity was not altered in dystrophic muscles. These findings indicate the occurrence in human dystrophic muscles of an increased level of lipid peroxidation and the possible activation of certain enzymes that could conceivably inhibit lipid peroxidation in vivo."}
{"id": "PMID:466817", "title": "Relationship of thyroid status and serum N-acetyl-beta-glucosaminidase isoenzyme activities in humans.", "content": "Serum from 28 hyperthyroid, hypothyroid, and euthyroid pre- or early puberty females was examined for N-acetyl-beta-glucosaminidase (HEX) activity. Total, isoenzyme A (labile), and isoenzyme B (stable) levels were determined for this enzyme. A high degree of correlation (r = 0.76; p less than 0.001) exists between total hexosaminidase activity and thyroid hormone levels (as reflected by the Free Thyroxine Index). Examining each isoenzyme individually, A is selectively enhanced (r = 0.84; p less than 0.0005) whereas B displays no significant change regardless of thyroid activity. In hyperthyroid individuals, levels of total hexosaminidase (730 +/- 67 units) (mean +/- S.D.) and isoenzyme A (516 +/- 46) were significantly higher than levels found in either the hypothyroid (total: 547 +/- 30; isoenzyme A: 352 +/- 31) or euthyroid (total: 620 +/- 81; isoenzyme A: 423 +/- 45) groups. However, no change was observed in levels of isoenzyme B among hypothyroid (195 +/- 19), euthyroid (197 +/- 43) and hyperthyroid (215 +/- 32) groups. These data substantiate our earlier findings in the rat, wherein thyroxine administration evoked a similar response in the liver. They are of particular interest in light of the deficiency of HEX A in variants of GM2 gangliosidosis.", "contents": "Relationship of thyroid status and serum N-acetyl-beta-glucosaminidase isoenzyme activities in humans. Serum from 28 hyperthyroid, hypothyroid, and euthyroid pre- or early puberty females was examined for N-acetyl-beta-glucosaminidase (HEX) activity. Total, isoenzyme A (labile), and isoenzyme B (stable) levels were determined for this enzyme. A high degree of correlation (r = 0.76; p less than 0.001) exists between total hexosaminidase activity and thyroid hormone levels (as reflected by the Free Thyroxine Index). Examining each isoenzyme individually, A is selectively enhanced (r = 0.84; p less than 0.0005) whereas B displays no significant change regardless of thyroid activity. In hyperthyroid individuals, levels of total hexosaminidase (730 +/- 67 units) (mean +/- S.D.) and isoenzyme A (516 +/- 46) were significantly higher than levels found in either the hypothyroid (total: 547 +/- 30; isoenzyme A: 352 +/- 31) or euthyroid (total: 620 +/- 81; isoenzyme A: 423 +/- 45) groups. However, no change was observed in levels of isoenzyme B among hypothyroid (195 +/- 19), euthyroid (197 +/- 43) and hyperthyroid (215 +/- 32) groups. These data substantiate our earlier findings in the rat, wherein thyroxine administration evoked a similar response in the liver. They are of particular interest in light of the deficiency of HEX A in variants of GM2 gangliosidosis."}
{"id": "PMID:466818", "title": "Use of small samples of urine to monitor gonadotrophins in menopausal women.", "content": "The increased gonadotrophin secretion which occurs at the menopause was followed by measuring gonadotrophin to creatinine ratios in small samples of urine. For follicle stimulating hormone, values of the ratio corresponded closely with both plasma concentration (correlation coefficients: 0.93, 0.94 and 0.93 for first morning, mid-morning and mid-afternoon urine samples: all results expressed as logarithms) and 24-h urine output (0.98, 0.96 and 0.94). Comparable figures for luteinising hormone (LH) were 0.94, 0.94 and 0.90; and 0.95, 0.95 and 0.90. Multiplication of the gonadotrophin to creatinine ratios (I.U./mmol) by 10 gave a figure close to 24-h gonadotrophin output (I.U.).", "contents": "Use of small samples of urine to monitor gonadotrophins in menopausal women. The increased gonadotrophin secretion which occurs at the menopause was followed by measuring gonadotrophin to creatinine ratios in small samples of urine. For follicle stimulating hormone, values of the ratio corresponded closely with both plasma concentration (correlation coefficients: 0.93, 0.94 and 0.93 for first morning, mid-morning and mid-afternoon urine samples: all results expressed as logarithms) and 24-h urine output (0.98, 0.96 and 0.94). Comparable figures for luteinising hormone (LH) were 0.94, 0.94 and 0.90; and 0.95, 0.95 and 0.90. Multiplication of the gonadotrophin to creatinine ratios (I.U./mmol) by 10 gave a figure close to 24-h gonadotrophin output (I.U.)."}
{"id": "PMID:466819", "title": "Immobilized enzyme nylon tube reactors: creatininase and creatine kinase linked to the pyruvate kinase-lactate dehydrogenase indicator system in the detection of creatinine and creatine.", "content": "A four enzyme system catalyzing a sequence of reactions was immobilized onto the inside surfaces of nylon tubes and studied as a possible method of analysis for the first substrate, creatinine in the sequence of reactions. The system was first studied in solution as a guide to optimizing conditions for preparation and use of the immobilized enzyme nylon tube reactors. The scope and limitations of this approach to routine analysis is discussed.", "contents": "Immobilized enzyme nylon tube reactors: creatininase and creatine kinase linked to the pyruvate kinase-lactate dehydrogenase indicator system in the detection of creatinine and creatine. A four enzyme system catalyzing a sequence of reactions was immobilized onto the inside surfaces of nylon tubes and studied as a possible method of analysis for the first substrate, creatinine in the sequence of reactions. The system was first studied in solution as a guide to optimizing conditions for preparation and use of the immobilized enzyme nylon tube reactors. The scope and limitations of this approach to routine analysis is discussed."}
{"id": "PMID:466820", "title": "Immobilized Enzyme Pipette, Impette. Routine estimation of serum and urine urea.", "content": "This paper describes the preparation, properties, principles of operation and use of a new device, Immobilized Enzyme Pipette or Impette in routine determination of blood and urine urea. Clinical trials carried out in a medium sized hospital gave very reliable and reproducible results with a very high precision.", "contents": "Immobilized Enzyme Pipette, Impette. Routine estimation of serum and urine urea. This paper describes the preparation, properties, principles of operation and use of a new device, Immobilized Enzyme Pipette or Impette in routine determination of blood and urine urea. Clinical trials carried out in a medium sized hospital gave very reliable and reproducible results with a very high precision."}
{"id": "PMID:466821", "title": "Abnormalities of erythrocyte membranes in biliary atresia: ultrastructure and lipid composition.", "content": "Biochemical and ultrastructural studies of red blood cell membranes from seven patients with congenital biliary atresia have been performed. Scanning electron microscopic observations revealed that more than half of these cells were of abnormal shapes, such as target, spur and cup-formed cells. By freeze-fracture electron microscopy, membrane particle-free smooth areas were noted in the fracture faces. In addition, the number of membrane-associated particles was 20% less than those in control subjects. The lipid analysis of red blood cells showed a significant increase in phospholipid and a marked increase in cholesterol content. The increase of phospholipid content was primarily caused by the increase of lecithin. The acyl chain analysis of erythrocyte lecithin demonstrated an increase in palmitic, palmitoleic and oleic acid, and a decrease in stearic and linoleic acid. These observations are similar to those of acquired biliary obstruction. The fluidity of erythrocyte membrane lipid, studied by a fluorescence technique using fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), was found to be less in an individual with congenital biliary atresia than in the control subject.", "contents": "Abnormalities of erythrocyte membranes in biliary atresia: ultrastructure and lipid composition. Biochemical and ultrastructural studies of red blood cell membranes from seven patients with congenital biliary atresia have been performed. Scanning electron microscopic observations revealed that more than half of these cells were of abnormal shapes, such as target, spur and cup-formed cells. By freeze-fracture electron microscopy, membrane particle-free smooth areas were noted in the fracture faces. In addition, the number of membrane-associated particles was 20% less than those in control subjects. The lipid analysis of red blood cells showed a significant increase in phospholipid and a marked increase in cholesterol content. The increase of phospholipid content was primarily caused by the increase of lecithin. The acyl chain analysis of erythrocyte lecithin demonstrated an increase in palmitic, palmitoleic and oleic acid, and a decrease in stearic and linoleic acid. These observations are similar to those of acquired biliary obstruction. The fluidity of erythrocyte membrane lipid, studied by a fluorescence technique using fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), was found to be less in an individual with congenital biliary atresia than in the control subject."}
{"id": "PMID:466840", "title": "Orthostatic postural activity disorders recorded by statokinesimeter in post-concussional syndromes: oculomoter aspect.", "content": "The purpose of this paper is to emphasize the influence of the mesencephalic oculomotor system regulation on orthostatic postural regulation. The body at rest is never immobile but it swings continuosly. The amplitudes and the frequencies of body sway give an indication of the function of the different sensorimotor loops which are involved in body balance. An apparatus, the statokinesimeter, allows us to record these motions by means of displacement of the body's centre of gravity with respect to the centre of the basis. In the case of head or cervical injuries, the brain stem is often implicated giving a partial nuclear unilateral lesion of the III nucleus; double vision of a few degrees with picture separation of never more than 4 degrees occurs. A compensory deviation of the head follows. A conflict between afferents from the III, IV, VI, VII, XI and supraspinal nuclei occurs. The amplitude and the frequency of the body sway change. A form of treatment is proposed for the 'pseudo-vertigo' of post-concussional syndrome.", "contents": "Orthostatic postural activity disorders recorded by statokinesimeter in post-concussional syndromes: oculomoter aspect. The purpose of this paper is to emphasize the influence of the mesencephalic oculomotor system regulation on orthostatic postural regulation. The body at rest is never immobile but it swings continuosly. The amplitudes and the frequencies of body sway give an indication of the function of the different sensorimotor loops which are involved in body balance. An apparatus, the statokinesimeter, allows us to record these motions by means of displacement of the body's centre of gravity with respect to the centre of the basis. In the case of head or cervical injuries, the brain stem is often implicated giving a partial nuclear unilateral lesion of the III nucleus; double vision of a few degrees with picture separation of never more than 4 degrees occurs. A compensory deviation of the head follows. A conflict between afferents from the III, IV, VI, VII, XI and supraspinal nuclei occurs. The amplitude and the frequency of the body sway change. A form of treatment is proposed for the 'pseudo-vertigo' of post-concussional syndrome."}
{"id": "PMID:466841", "title": "Lateral cervical anomaliew and salivary heterotopia.", "content": "It is widely accepted that most lateral cervical fistulas with an external opening in the suprasternal region orginate from second branchial arch remnants. Lateral cervical cysts occur higher in the neck and according to current theories, may develop either from the branchial apparatus or from glandular inclusions in cervical lymph nodes. An histological study revealed salivary tissue in 9 of 24 fistulas, 5 of these representing a congenital cervical salivary sinus, while only one of 17 cysts showed salivary heterotopia. This investigation indicates that salivary tissue may occur more commonly in the lateral neck than is generally realised, and that lateral cervical cystis and fistulas probably differ in their histogenesis.", "contents": "Lateral cervical anomaliew and salivary heterotopia. It is widely accepted that most lateral cervical fistulas with an external opening in the suprasternal region orginate from second branchial arch remnants. Lateral cervical cysts occur higher in the neck and according to current theories, may develop either from the branchial apparatus or from glandular inclusions in cervical lymph nodes. An histological study revealed salivary tissue in 9 of 24 fistulas, 5 of these representing a congenital cervical salivary sinus, while only one of 17 cysts showed salivary heterotopia. This investigation indicates that salivary tissue may occur more commonly in the lateral neck than is generally realised, and that lateral cervical cystis and fistulas probably differ in their histogenesis."}
{"id": "PMID:466842", "title": "Bell's palsy; prognostic accuracy of case history, sialometry and taste impairment.", "content": "A prognostic test in cases of Bell's palsy must be accurate and applicable early in the course of the disease. The case history is examined in 239 patients with Bell's palsy. The prognostic value of the case history, taste impairment and sialometry is investigated in a series of untreated patients who were followed up for 12 months. Sialometry is advocated as a reliable progostic indicator in the individual patient if a strong stimulant is used. None of the other examined parameters discrimates between good and poor outcome with reasonable accuracy. A follow-up time of 12 months is advocated in determining the ultimate outcome of a Bell's palsy.", "contents": "Bell's palsy; prognostic accuracy of case history, sialometry and taste impairment. A prognostic test in cases of Bell's palsy must be accurate and applicable early in the course of the disease. The case history is examined in 239 patients with Bell's palsy. The prognostic value of the case history, taste impairment and sialometry is investigated in a series of untreated patients who were followed up for 12 months. Sialometry is advocated as a reliable progostic indicator in the individual patient if a strong stimulant is used. None of the other examined parameters discrimates between good and poor outcome with reasonable accuracy. A follow-up time of 12 months is advocated in determining the ultimate outcome of a Bell's palsy."}
{"id": "PMID:466843", "title": "Physiological changes in the treatment of acrophobia (fear of height).", "content": "In order to investigate the physiological changes produced by the treatment of acrophobic patients body movement and Microvibration were measured before and after treatment. Eighteen acrophobic patients were assigned at random to 1 of the 2 groups: a treatment group (n = 8) and a non-treatment group (n = 10). The control group consisted of 16 healthy volunteers. Body movement area while viewing a slide of a high place or imagining a high place in the treatment group decreased significantly after treatment. Body movement of the control group showed almost no changes, and that of the non-treatment group was situated between the above-mentioned 2 groups. Simple body movements of the acrophobic patients without any stimulation of height were bigger than those of the control subjects. MV pattern of the treatment group had a tendency to improve under psycho-therapy. Acrophobic patients had more abnormal MV patterns than the normal subjects.", "contents": "Physiological changes in the treatment of acrophobia (fear of height). In order to investigate the physiological changes produced by the treatment of acrophobic patients body movement and Microvibration were measured before and after treatment. Eighteen acrophobic patients were assigned at random to 1 of the 2 groups: a treatment group (n = 8) and a non-treatment group (n = 10). The control group consisted of 16 healthy volunteers. Body movement area while viewing a slide of a high place or imagining a high place in the treatment group decreased significantly after treatment. Body movement of the control group showed almost no changes, and that of the non-treatment group was situated between the above-mentioned 2 groups. Simple body movements of the acrophobic patients without any stimulation of height were bigger than those of the control subjects. MV pattern of the treatment group had a tendency to improve under psycho-therapy. Acrophobic patients had more abnormal MV patterns than the normal subjects."}
{"id": "PMID:466844", "title": "BIPP induced artefacts on computerized tomography.", "content": "A case of BIPP induced artefacts on computerized tomography (CT) is presented. Because of the value of CT as a non-invasive intra-cranial investigation, the use of radio-opaque materials in the site to be investigated is to be avoided. In cases likely to require the use of CT, a substitute for BIPP packs in ear surgery is now required.", "contents": "BIPP induced artefacts on computerized tomography. A case of BIPP induced artefacts on computerized tomography (CT) is presented. Because of the value of CT as a non-invasive intra-cranial investigation, the use of radio-opaque materials in the site to be investigated is to be avoided. In cases likely to require the use of CT, a substitute for BIPP packs in ear surgery is now required."}
{"id": "PMID:466846", "title": "Lyon phenomenon in ouabain-treated erythrocytes of Duchenne muscular dystrophy carriers as revealed by cell electrophoresis.", "content": "Using the cell-electrophoretic method, 0.1 mM ouabain/l Eagle MEM medium caused a moderate decrease in electrophoretic migration time (EMT) of erythrocytes in 19 out of 23 DMD-affected patients. In 8 out of 10 healthy boys, 0.1 mM ouabain induced an increase in erythrocyte EMT. Under the influence of 0.1 mM ouabain, the erythrocytes clearly showed a bimodal distribution of EMT in 9 out of 13 definite carriers and in 8 out of 17 possible carriers. In DMD patients, and in 10 healthy boys and 10 healthy women, no bimodal distribution of EMT was observed.", "contents": "Lyon phenomenon in ouabain-treated erythrocytes of Duchenne muscular dystrophy carriers as revealed by cell electrophoresis. Using the cell-electrophoretic method, 0.1 mM ouabain/l Eagle MEM medium caused a moderate decrease in electrophoretic migration time (EMT) of erythrocytes in 19 out of 23 DMD-affected patients. In 8 out of 10 healthy boys, 0.1 mM ouabain induced an increase in erythrocyte EMT. Under the influence of 0.1 mM ouabain, the erythrocytes clearly showed a bimodal distribution of EMT in 9 out of 13 definite carriers and in 8 out of 17 possible carriers. In DMD patients, and in 10 healthy boys and 10 healthy women, no bimodal distribution of EMT was observed."}
{"id": "PMID:466847", "title": "A pericentric inversion of chromosome 9 and a rearrangement involving chromosomes 9 and 10, observed in two generations. Clinical description of chromosome 9 (p12-p21) deletion syndrome.", "content": "The clinical picture associated with a deletion of a central part of the short arm of chromosome no. 9 is described in two siblings. The clinical signs differ from those described in deletion of the terminal part of the short arm. Pericentric inversion of chromosome no. 9, combined with a rearrangement involving chromosomes 9 and 10, was found in the mother and the maternal grandmother of the propositus.", "contents": "A pericentric inversion of chromosome 9 and a rearrangement involving chromosomes 9 and 10, observed in two generations. Clinical description of chromosome 9 (p12-p21) deletion syndrome. The clinical picture associated with a deletion of a central part of the short arm of chromosome no. 9 is described in two siblings. The clinical signs differ from those described in deletion of the terminal part of the short arm. Pericentric inversion of chromosome no. 9, combined with a rearrangement involving chromosomes 9 and 10, was found in the mother and the maternal grandmother of the propositus."}
{"id": "PMID:466848", "title": "High alkaline phosphatase activity in isoproterenol stimulated fibroblast cultures from patients with numerically unbalanced chromosomal aberrations.", "content": "Treatment of cultured fibroblasts from patients with unbalanced chromosomal aberrations with a mixture of isoproterenol, theophylline and ascorbic acid resulted after 48 hours in an at least three-fold increase of alkaline phosphatase activity on a per cell basis, whereas cells from normal healthy individuals did not show this dramatic response. Cells were studied from patients with trisomy 21 (14 cases), trisomy 18 (3 cases), trisomy 13 (1 case), pentasomy X (1 case), Turner syndrome (2 cases), and Klinefelter syndrome (1 case), and no exception was noted. The mechanism of this phenomenon is not clear, but it is speculated that increased cyclic-AMP levels caused by the action of isoproterenol on adenylcyclase may account for excessive reactions of unbalanced cells as compared to normal cells. This simple biochemical diagnostic procedure might become useful in screening programs for unbalanced chromosomal abberations.", "contents": "High alkaline phosphatase activity in isoproterenol stimulated fibroblast cultures from patients with numerically unbalanced chromosomal aberrations. Treatment of cultured fibroblasts from patients with unbalanced chromosomal aberrations with a mixture of isoproterenol, theophylline and ascorbic acid resulted after 48 hours in an at least three-fold increase of alkaline phosphatase activity on a per cell basis, whereas cells from normal healthy individuals did not show this dramatic response. Cells were studied from patients with trisomy 21 (14 cases), trisomy 18 (3 cases), trisomy 13 (1 case), pentasomy X (1 case), Turner syndrome (2 cases), and Klinefelter syndrome (1 case), and no exception was noted. The mechanism of this phenomenon is not clear, but it is speculated that increased cyclic-AMP levels caused by the action of isoproterenol on adenylcyclase may account for excessive reactions of unbalanced cells as compared to normal cells. This simple biochemical diagnostic procedure might become useful in screening programs for unbalanced chromosomal abberations."}
{"id": "PMID:466849", "title": "Prenatal diagnosis of thrombocytopenia with absent radii.", "content": "Six pregnancies at risk for thrombocytopenia and absent radii (TAR) have been studied between 16 and 20 menstrual weeks utilizing fetal radiography. Two affected and four unaffected fetuses have been correctly identified. Fetal radiography can provide reliable prenatal diagnosis in pregnancies at risk for TAR.", "contents": "Prenatal diagnosis of thrombocytopenia with absent radii. Six pregnancies at risk for thrombocytopenia and absent radii (TAR) have been studied between 16 and 20 menstrual weeks utilizing fetal radiography. Two affected and four unaffected fetuses have been correctly identified. Fetal radiography can provide reliable prenatal diagnosis in pregnancies at risk for TAR."}
{"id": "PMID:466850", "title": "Chromosomes in retinoblastoma patients.", "content": "In a series of eight patients with retinoblastoma, one was found to have a reciprocal translocation of chromosomes 1 and 13. The breakpoint on chromosome 13 is at band q12, which suggests that the retinoblastoma locus is less distal than previously thought.", "contents": "Chromosomes in retinoblastoma patients. In a series of eight patients with retinoblastoma, one was found to have a reciprocal translocation of chromosomes 1 and 13. The breakpoint on chromosome 13 is at band q12, which suggests that the retinoblastoma locus is less distal than previously thought."}
{"id": "PMID:466851", "title": "Additions to the myotonic dystrophy linkage group.", "content": "One hundred and thirty members of a family with 33 cases of Dystrophia myotonica (Dm) were tested for various genetic markers including Km, Jk, Lu and Se. All individuals tested were Lua negative, but many were heterozygous for the other markers. Except for two cases all patients with Dm were positive for Km3 and Jka and they were secretors. Assuming the genes are linked and that the order of the loci is Km, Jk, Lu, Se, Dm, then there were 22 meioses informative for Dm and in 10 of these a recombination must have occurred between two of the five loci. Looking at individual pairs of loci, there were no recombinations between Km and Jk in seven informative meioses, three recombinations out of 10 meioses informative for Jk/Se, no recombinations between Se and Dm in five informative meioses and three recombinations out of 12 meioses informative for Jk/Dm.", "contents": "Additions to the myotonic dystrophy linkage group. One hundred and thirty members of a family with 33 cases of Dystrophia myotonica (Dm) were tested for various genetic markers including Km, Jk, Lu and Se. All individuals tested were Lua negative, but many were heterozygous for the other markers. Except for two cases all patients with Dm were positive for Km3 and Jka and they were secretors. Assuming the genes are linked and that the order of the loci is Km, Jk, Lu, Se, Dm, then there were 22 meioses informative for Dm and in 10 of these a recombination must have occurred between two of the five loci. Looking at individual pairs of loci, there were no recombinations between Km and Jk in seven informative meioses, three recombinations out of 10 meioses informative for Jk/Se, no recombinations between Se and Dm in five informative meioses and three recombinations out of 12 meioses informative for Jk/Dm."}
{"id": "PMID:466852", "title": "Satellite DNA loss and nucleolar organiser activity in an individual with a de novo chromosome 13,14 translocation.", "content": "The distribution of satellite DNA and nucleolar organiser activity have been studied in a female with a new dicentric translocation chromosome derived from the maternal chromosomes 13 and 14. More than half the satellite DNA (60.5%) was lost in the translocation, together with both the nucleolar organiser regions (NOR'S). However, at least one NOR (chromosome 21) which was inactive in the mother (by the AgI reaction) is active in the subject, and this may be an example of functional compensation. The somatic cells of the mother of the subject, which do not have the translocation, show a high frequency of acrocentric associations, but these do not include any obvious excess of associations involving chromosomes 13 and 14, indicating that the high frequency of association in somatic cells is not in itself a predisposition to Robertsonian translocation in germ line cells. The father's chromosomes 9 both have more satellite DNA in the secondary constriction than normal, but this is not reflected in any obviously larger size of the C-band in this region.", "contents": "Satellite DNA loss and nucleolar organiser activity in an individual with a de novo chromosome 13,14 translocation. The distribution of satellite DNA and nucleolar organiser activity have been studied in a female with a new dicentric translocation chromosome derived from the maternal chromosomes 13 and 14. More than half the satellite DNA (60.5%) was lost in the translocation, together with both the nucleolar organiser regions (NOR'S). However, at least one NOR (chromosome 21) which was inactive in the mother (by the AgI reaction) is active in the subject, and this may be an example of functional compensation. The somatic cells of the mother of the subject, which do not have the translocation, show a high frequency of acrocentric associations, but these do not include any obvious excess of associations involving chromosomes 13 and 14, indicating that the high frequency of association in somatic cells is not in itself a predisposition to Robertsonian translocation in germ line cells. The father's chromosomes 9 both have more satellite DNA in the secondary constriction than normal, but this is not reflected in any obviously larger size of the C-band in this region."}
{"id": "PMID:466853", "title": "Partial trisomy 1q syndrome.", "content": "Six cases of partial trisomy 1q, including four cases from the literature and our own two observations are summarized with respect to their clinical symptoms. Distinct similarities of the external aspect and of internal malformations allow the delineation of a syndrome of partial trisomy 1q.", "contents": "Partial trisomy 1q syndrome. Six cases of partial trisomy 1q, including four cases from the literature and our own two observations are summarized with respect to their clinical symptoms. Distinct similarities of the external aspect and of internal malformations allow the delineation of a syndrome of partial trisomy 1q."}
{"id": "PMID:466856", "title": "Histochemical studies for 5'-nucleotidase and alpha-naphthyl (non-specific) esterase in lymphocytes from patients with primary immunoglobulin deficiencies.", "content": "Lymphocytes from patients with primary immunodeficiency were tested histochemically for ecto 5'-nucleotidase (5'N) and alpha-naphthyl (non-specific) esterase. More than half the patients with 'common variable' hypogammaglobulinaemia, all those with X-linked hypogammaglobulinaemia and some of those with selective IgA deficiency had a very low percentage of lymphocytes staining for 5'N as compared to controls. A lack of B cells probably explains the finding in X-linked hypogammaglobulinaemia, but does not fully explain the results in the other groups. Most patients with 'common variable' hypogammaglobulinaemia had a very low percentage of lymphocytes with granular staining for alpha-naphthyl (non-specific) esterase in contrast to normal numbers in those with X-linked hypogammaglobulinaemia and most of those with selective IgA deficiency. Granules containing non-specific esterase are characteristically found in 'mature' T lymphocytes. The enzyme abnormalities in the T and B cells of 'common variable' hypogammaglobulinaemic patients could be explained by 'immature' cell types.", "contents": "Histochemical studies for 5'-nucleotidase and alpha-naphthyl (non-specific) esterase in lymphocytes from patients with primary immunoglobulin deficiencies. Lymphocytes from patients with primary immunodeficiency were tested histochemically for ecto 5'-nucleotidase (5'N) and alpha-naphthyl (non-specific) esterase. More than half the patients with 'common variable' hypogammaglobulinaemia, all those with X-linked hypogammaglobulinaemia and some of those with selective IgA deficiency had a very low percentage of lymphocytes staining for 5'N as compared to controls. A lack of B cells probably explains the finding in X-linked hypogammaglobulinaemia, but does not fully explain the results in the other groups. Most patients with 'common variable' hypogammaglobulinaemia had a very low percentage of lymphocytes with granular staining for alpha-naphthyl (non-specific) esterase in contrast to normal numbers in those with X-linked hypogammaglobulinaemia and most of those with selective IgA deficiency. Granules containing non-specific esterase are characteristically found in 'mature' T lymphocytes. The enzyme abnormalities in the T and B cells of 'common variable' hypogammaglobulinaemic patients could be explained by 'immature' cell types."}
{"id": "PMID:466857", "title": "Quantitative and qualitative investigations of serum IgG subclasses in immunodeficiency diseases.", "content": "Determinations of IgG subclasses were made by electroimmunoassay and crossed immunoelectrophoresis, and Gm markers were typed in sera from seventeen patients with well-defined immunodeficiency diseases. Certain IgG subclass and Gm patterns were recognized in various diseases: IgG2 deficiency and homozygosity of Gm (4,5) in the cartilage-hair-hypoplasia syndrome, in the ataxia telangiectasia syndrome and in selective IgG subclass deficiency; and IgG3 deficiency and homozygosity of Gm(1,-5) in the Wiskott-Aldrich syndrome. The findings suggest a common structural or regulator gene defect in some immunodeficiency diseases. In IgA deficiencies, the levels of IgG1 were raised. In patients with IgG subclass deficiencies there was sometimes a compensatory increase of the remaining IgG subclasses, with a preponderance of IgG1 and IgG3. The increased Ig1 showed restricted heterogeneity with only an increase of the electrophoretically cathodal part. This part contained both kappa and lambda chaings. IgG subclass deficiency indicates treatment with gammaglobulin even if the serum levels of IgG are normal or increased.", "contents": "Quantitative and qualitative investigations of serum IgG subclasses in immunodeficiency diseases. Determinations of IgG subclasses were made by electroimmunoassay and crossed immunoelectrophoresis, and Gm markers were typed in sera from seventeen patients with well-defined immunodeficiency diseases. Certain IgG subclass and Gm patterns were recognized in various diseases: IgG2 deficiency and homozygosity of Gm (4,5) in the cartilage-hair-hypoplasia syndrome, in the ataxia telangiectasia syndrome and in selective IgG subclass deficiency; and IgG3 deficiency and homozygosity of Gm(1,-5) in the Wiskott-Aldrich syndrome. The findings suggest a common structural or regulator gene defect in some immunodeficiency diseases. In IgA deficiencies, the levels of IgG1 were raised. In patients with IgG subclass deficiencies there was sometimes a compensatory increase of the remaining IgG subclasses, with a preponderance of IgG1 and IgG3. The increased Ig1 showed restricted heterogeneity with only an increase of the electrophoretically cathodal part. This part contained both kappa and lambda chaings. IgG subclass deficiency indicates treatment with gammaglobulin even if the serum levels of IgG are normal or increased."}
{"id": "PMID:466858", "title": "Complement activation in asymptomatic patients with sickle cell anaemia.", "content": "Previous reports have suggested that a defect in serum complement may contribute to the increased susceptibility to infection shown by patients with sickle cell anaemia (SCA). In order to define the nature of any complement abnormality in SCA, we investigated the complement system in eighty-seven patients during asymptomatic periods, and analysed factor B turnover in a small sample. In these patients geometric mean serum concentrations of functionally active factor B and factor D, and of C3 and C4 protein (expressed as a percentage of normal reference serum) wer lower than in controls (78% vs 107%, P less than 0.001, 86% vs 103%, P less than 0.001, 91% vs 100%, P less than 0.01, 89% vs 105%, P less than 0.05 respectively). The ratio of the serum concentration of functionally active factor B to factor B protein was lower in patients than in controls (means 75% s.d. 16% vs mean 93%, s.d. 22% P less than 0.001), indicating a functional deficiency of factor B protein. In addition, the fractional catabolic rate of radiolabelled factor B was markedly increased in four out of seven asymptomatic patients studied, and was inversely related to the functional factor B concentration in serum (r = -0.59, P less than 0.05); factor B synthesis was uniformly increased. Complement activation was not related to the presence of circulating C1q binding material. We conclude that complement activation, rather than defective synthesis as previously suggested, contributes to the abnormalities in complement componenet concentration and function in asymptomatic subjects with sickle cell anaemia.", "contents": "Complement activation in asymptomatic patients with sickle cell anaemia. Previous reports have suggested that a defect in serum complement may contribute to the increased susceptibility to infection shown by patients with sickle cell anaemia (SCA). In order to define the nature of any complement abnormality in SCA, we investigated the complement system in eighty-seven patients during asymptomatic periods, and analysed factor B turnover in a small sample. In these patients geometric mean serum concentrations of functionally active factor B and factor D, and of C3 and C4 protein (expressed as a percentage of normal reference serum) wer lower than in controls (78% vs 107%, P less than 0.001, 86% vs 103%, P less than 0.001, 91% vs 100%, P less than 0.01, 89% vs 105%, P less than 0.05 respectively). The ratio of the serum concentration of functionally active factor B to factor B protein was lower in patients than in controls (means 75% s.d. 16% vs mean 93%, s.d. 22% P less than 0.001), indicating a functional deficiency of factor B protein. In addition, the fractional catabolic rate of radiolabelled factor B was markedly increased in four out of seven asymptomatic patients studied, and was inversely related to the functional factor B concentration in serum (r = -0.59, P less than 0.05); factor B synthesis was uniformly increased. Complement activation was not related to the presence of circulating C1q binding material. We conclude that complement activation, rather than defective synthesis as previously suggested, contributes to the abnormalities in complement componenet concentration and function in asymptomatic subjects with sickle cell anaemia."}
{"id": "PMID:466859", "title": "Effect of sex hormones on the immune system of guinea-pigs and on the development of toxoplasmic lesions in non-lymphoid organs.", "content": "The role of cell-mediated immunity in the pathogenesis of toxoplasmic lesions in non-lymphoid organs and its significance in resistance to infection with Toxoplasma gondii were studied in guinea-pigs. Alteration of the sex hormonal environment in both sexes has significant effects on the immunological status of the guinea-pig, changing its reaction to toxoplasmic infection. Thus, gonadectomy appears to enhance delayed hypersensitivity, leading to a greater prominence of lesions in the non-lymphoid organs of gonadectomized male and female animals than in controls. This observation supports the possibility that delayed hypersensitivity plays a major role in the pathogenesis of non-lymphoid, toxoplasmic lesions. Hexoestrol administration for a long period of time induces complete thymic atrophy and severe involution of the thymus-dependent areas of the lymph nodes and spleen, leading to the suppression of cell-mediated immunity and overwhelming disease in those animals infected with Toxoplasma gondii, suggesting that cell-mediated immunity is of importance in resistance to toxoplasmic infection. In view of these findings, the difficulties in the treatment of non-lymphoid toxoplasmic lesions, such as ocular ones, by depressing cellular immunity, are discussed.", "contents": "Effect of sex hormones on the immune system of guinea-pigs and on the development of toxoplasmic lesions in non-lymphoid organs. The role of cell-mediated immunity in the pathogenesis of toxoplasmic lesions in non-lymphoid organs and its significance in resistance to infection with Toxoplasma gondii were studied in guinea-pigs. Alteration of the sex hormonal environment in both sexes has significant effects on the immunological status of the guinea-pig, changing its reaction to toxoplasmic infection. Thus, gonadectomy appears to enhance delayed hypersensitivity, leading to a greater prominence of lesions in the non-lymphoid organs of gonadectomized male and female animals than in controls. This observation supports the possibility that delayed hypersensitivity plays a major role in the pathogenesis of non-lymphoid, toxoplasmic lesions. Hexoestrol administration for a long period of time induces complete thymic atrophy and severe involution of the thymus-dependent areas of the lymph nodes and spleen, leading to the suppression of cell-mediated immunity and overwhelming disease in those animals infected with Toxoplasma gondii, suggesting that cell-mediated immunity is of importance in resistance to toxoplasmic infection. In view of these findings, the difficulties in the treatment of non-lymphoid toxoplasmic lesions, such as ocular ones, by depressing cellular immunity, are discussed."}
{"id": "PMID:466860", "title": "Antibody-dependent and phytohaemagglutinin-induced lymphocyte cytotoxicity in systemic sclerosis.", "content": "Cell-mediated cytotoxicity was examined in thirty-seven patients with systemic sclerosis using both whole blood and purified peripheral blood mononuclear cells (PBM) to measure antibody-dependent (ADCC) and phytohaemagglutinin (PHA) induced lymphocyte cytotoxicity to 51Cr-labelled Chang liver cells. In twenty-three mildly affected patients, ADCC and PHA-induced cytotoxicity did not differ from that found in control populations. By contrast, fourteen patients severely affected by extensive visceral disease showed reductions in both ADCC and PHA-induced cytotoxicity which were more marked in whole blood assays (P less than 0.001) than in those performed with PBM (P less than 0.05). The addition of patient's sera to control cytotoxicity assays suggested that blocking or suppressive serum factors could only account for some of the disproportionate reduction in whole blood cytotoxicity which, in the main, must be due to a lack of circulating effector cells. These results are in agreement with previous findings of reduced numbers of circulating thymus-dependent lymphocytes in patients with severe disease, a defect of cell-mediated immunity that may result from the chronic antigenic stimulation of an autoimmune disease process.", "contents": "Antibody-dependent and phytohaemagglutinin-induced lymphocyte cytotoxicity in systemic sclerosis. Cell-mediated cytotoxicity was examined in thirty-seven patients with systemic sclerosis using both whole blood and purified peripheral blood mononuclear cells (PBM) to measure antibody-dependent (ADCC) and phytohaemagglutinin (PHA) induced lymphocyte cytotoxicity to 51Cr-labelled Chang liver cells. In twenty-three mildly affected patients, ADCC and PHA-induced cytotoxicity did not differ from that found in control populations. By contrast, fourteen patients severely affected by extensive visceral disease showed reductions in both ADCC and PHA-induced cytotoxicity which were more marked in whole blood assays (P less than 0.001) than in those performed with PBM (P less than 0.05). The addition of patient's sera to control cytotoxicity assays suggested that blocking or suppressive serum factors could only account for some of the disproportionate reduction in whole blood cytotoxicity which, in the main, must be due to a lack of circulating effector cells. These results are in agreement with previous findings of reduced numbers of circulating thymus-dependent lymphocytes in patients with severe disease, a defect of cell-mediated immunity that may result from the chronic antigenic stimulation of an autoimmune disease process."}
{"id": "PMID:466861", "title": "The quantification of viable Leishmania enriettii from infected guinea-pig tissues.", "content": "Results are presented demonstrating that in vitro cultivation of Leishmania enrietti can be used to determine the level of viability in suspensions of L. enrietti used for infection and also to quantify the number of viable organisms in infected tissues.", "contents": "The quantification of viable Leishmania enriettii from infected guinea-pig tissues. Results are presented demonstrating that in vitro cultivation of Leishmania enrietti can be used to determine the level of viability in suspensions of L. enrietti used for infection and also to quantify the number of viable organisms in infected tissues."}
{"id": "PMID:466862", "title": "Immunological aspects of cryoprecipitates from the sera of chronic HBsAg carriers.", "content": "The sera of chronic hepatitis B surface antigen (HBsAg) carriers and seropositive controls were examined for the presence of immune complexes by cryoprecipitation. Cryoprecipitates (CP) were tested for HBsAg, antibody to HBsAg (anti-HBs), major classes of immunoglobulins, components of the complement system, rheumatoid factor and the ability to activate the alternative pathway of the complement system. For this analysis the methods employed included: radioimmunoassay, reverse passive haemagglutination, immunofluorescence, sucrose density gradient ultracentrifugation, agar-gel diffusion, immunoelectrophoresis, counterimmunoelectrophoresis, latex agglutination, and a haemolytic method for the detection of the activation of the alternative pathway of the complement system. HBsAg was frequently observed in the CP from chronic HBsAg carriers. No anti-HBs activity was detected in the serum of chronic HBsAg carriers. However, the CP from a number of chronic HBsAg carriers contained immunoglobulins and components of the complement system in the absence of rheumatoid factor, anti-HBs activity and were able to activate the alternative pathway of the complement system. On immunoelectrophoresis, a component of the CP reacting with anti-IgG, ANTI-IgA and anti-HBs antisera and demonstrating an altered (faster) electrophoretic mobility was observed. The nature of the CP strongly suggests the presence of circulating immune complexes in asymptomatic chronic HBaAg carriers. These immune complexes may be important in the eventual expression and outcome of clinical disease in apparently healthy carriers of HBsAg.", "contents": "Immunological aspects of cryoprecipitates from the sera of chronic HBsAg carriers. The sera of chronic hepatitis B surface antigen (HBsAg) carriers and seropositive controls were examined for the presence of immune complexes by cryoprecipitation. Cryoprecipitates (CP) were tested for HBsAg, antibody to HBsAg (anti-HBs), major classes of immunoglobulins, components of the complement system, rheumatoid factor and the ability to activate the alternative pathway of the complement system. For this analysis the methods employed included: radioimmunoassay, reverse passive haemagglutination, immunofluorescence, sucrose density gradient ultracentrifugation, agar-gel diffusion, immunoelectrophoresis, counterimmunoelectrophoresis, latex agglutination, and a haemolytic method for the detection of the activation of the alternative pathway of the complement system. HBsAg was frequently observed in the CP from chronic HBsAg carriers. No anti-HBs activity was detected in the serum of chronic HBsAg carriers. However, the CP from a number of chronic HBsAg carriers contained immunoglobulins and components of the complement system in the absence of rheumatoid factor, anti-HBs activity and were able to activate the alternative pathway of the complement system. On immunoelectrophoresis, a component of the CP reacting with anti-IgG, ANTI-IgA and anti-HBs antisera and demonstrating an altered (faster) electrophoretic mobility was observed. The nature of the CP strongly suggests the presence of circulating immune complexes in asymptomatic chronic HBaAg carriers. These immune complexes may be important in the eventual expression and outcome of clinical disease in apparently healthy carriers of HBsAg."}
{"id": "PMID:466865", "title": "Temporal relationship between cardiac performance and myocardial uptake of dl-propranolol in man.", "content": "1. The temporal relationship between the myocardial arteriovenous difference of plasma propranolol concentration and heart rate and left ventricular dp/dt maximum was examined for 30 min after intravenous administration of 10mg (0.04 mmol) dl-propranolol in nine patients with ischaemic heart disease. 2. Very large positive myocardial a.v. differences of propranolol within the first 5 min became negative after 5 min, indicating a rapid, avid uptake with subsequent release of propranolol from the heart. 3. The heart rate and left ventricular dp/dt maximum increased 1 min after propranolol administration and did not change after the fifth min of the observation period. 4. The data suggest that a large number of unspecific binding sites for propranolol are present in the heart in addition to the specific beta-receptors.", "contents": "Temporal relationship between cardiac performance and myocardial uptake of dl-propranolol in man. 1. The temporal relationship between the myocardial arteriovenous difference of plasma propranolol concentration and heart rate and left ventricular dp/dt maximum was examined for 30 min after intravenous administration of 10mg (0.04 mmol) dl-propranolol in nine patients with ischaemic heart disease. 2. Very large positive myocardial a.v. differences of propranolol within the first 5 min became negative after 5 min, indicating a rapid, avid uptake with subsequent release of propranolol from the heart. 3. The heart rate and left ventricular dp/dt maximum increased 1 min after propranolol administration and did not change after the fifth min of the observation period. 4. The data suggest that a large number of unspecific binding sites for propranolol are present in the heart in addition to the specific beta-receptors."}
{"id": "PMID:466866", "title": "Drug interactions in cat isolated tracheal smooth muscle.", "content": "1. The interactions between some drugs that contract and relax airways smooth muscle have been investigated in the cat isolated trachea. 2. Isoprenaline and theophylline inhibited serotonin-elicited contractions more than acetylcholine-mediated responses. This was observed both in terms of the degree of inhibition and the concentration of the relaxant drug producing this inhibition. 3. The acetylcholine- and serotonin-induced contractions were inhibited more by theophylline than by isoprenaline displaced acetylcholine and serotonin response curves to the right whereas theophylline caused a flattening of the curves. 4. Isoprenaline was more effective in hibiting serotonin contractions than acetylcholine contractions when the tracheas were bathed in K+ depolarizing solution, suggesting that the difference in the suscepbibility of serotonin and acetylcholine contractions to isoprenaline was not dependent on the electrical membrane potential. 5. Isoprenaline inhibited the tonic component of acetylcholine contractions more than the phasic component. 6. The differences in the pharmacological responses to the contractile and relaxant drugs in cat tracheal preparations provide further examples in smooth muscle of different mechanisms by which acetylcholine and serotonin induce contraction and isoprenaline and theophylline relaxation.", "contents": "Drug interactions in cat isolated tracheal smooth muscle. 1. The interactions between some drugs that contract and relax airways smooth muscle have been investigated in the cat isolated trachea. 2. Isoprenaline and theophylline inhibited serotonin-elicited contractions more than acetylcholine-mediated responses. This was observed both in terms of the degree of inhibition and the concentration of the relaxant drug producing this inhibition. 3. The acetylcholine- and serotonin-induced contractions were inhibited more by theophylline than by isoprenaline displaced acetylcholine and serotonin response curves to the right whereas theophylline caused a flattening of the curves. 4. Isoprenaline was more effective in hibiting serotonin contractions than acetylcholine contractions when the tracheas were bathed in K+ depolarizing solution, suggesting that the difference in the suscepbibility of serotonin and acetylcholine contractions to isoprenaline was not dependent on the electrical membrane potential. 5. Isoprenaline inhibited the tonic component of acetylcholine contractions more than the phasic component. 6. The differences in the pharmacological responses to the contractile and relaxant drugs in cat tracheal preparations provide further examples in smooth muscle of different mechanisms by which acetylcholine and serotonin induce contraction and isoprenaline and theophylline relaxation."}
{"id": "PMID:466868", "title": "Measurement of clofibric acid (CPIB) metabolites in plasma of patients on clofibrate therapy.", "content": "1. The metabolites of clofibric acid [CPIB;2-(chlorophenoxy)-2-methylpropionic acid] are present in the plasma of patients on clofibrate therapy. The highest plasma concentrations of CPIB in metabolite form (up to 51 micrograms/ml) were generally found in patients with renal disease. Negligible concentrations (less than or equal to 2 micrograms/ml) were found in only seven patients out of thirty-six studied. 2. The two conjugates of CPIB found in urine were present in plasma. 3. When measuring conjugated CPIB in plasma it is essential to take care in the handling and storage of specimens, and to select an assay method known to be specific for unmetabolized CPIB.", "contents": "Measurement of clofibric acid (CPIB) metabolites in plasma of patients on clofibrate therapy. 1. The metabolites of clofibric acid [CPIB;2-(chlorophenoxy)-2-methylpropionic acid] are present in the plasma of patients on clofibrate therapy. The highest plasma concentrations of CPIB in metabolite form (up to 51 micrograms/ml) were generally found in patients with renal disease. Negligible concentrations (less than or equal to 2 micrograms/ml) were found in only seven patients out of thirty-six studied. 2. The two conjugates of CPIB found in urine were present in plasma. 3. When measuring conjugated CPIB in plasma it is essential to take care in the handling and storage of specimens, and to select an assay method known to be specific for unmetabolized CPIB."}
{"id": "PMID:466869", "title": "Occurrence of excitatory dopaminoceptors in the rat and guinea-pig vas deferens.", "content": "1. Noradrenaline and dopamine produce contractions of isolated preparations of vasa deferentia from the rat or the guinea-pig. 2. Dopamine was three to five times as potent as noradrenaline on the rat vas deferens, but was considerably less potent than noradrenaline on the guinea-pig vas deferens. 3. Phentolamine antagonized responses to noradrenaline and dopamine of vasa deferentia of both species. On the rat vas deferens the pA2 values against noradrenaline and dopamine were 7.6 and 7.4, respectively. 4. Pimozide, a dopamine antagonist, antagonized responses to dopamine on vasa deferentia of both species, but was not significantly active in antagonizing responses to noradrenaline. On the rat vas deferens, the pA2 value against dopamine was 7.9. t. The beta-adrenoceptor blocking agents, propranolol and oxprenolol slightly enhanced dopamine-induced contractions on vasa deferentia of both species. 6. It is suggested that dopamine might act on both a-adrenoceptors and dopaminoceptors in the vas deferens, and that the component due to activation of dopaminoceptors is greater in the rat than the guinea-pig.", "contents": "Occurrence of excitatory dopaminoceptors in the rat and guinea-pig vas deferens. 1. Noradrenaline and dopamine produce contractions of isolated preparations of vasa deferentia from the rat or the guinea-pig. 2. Dopamine was three to five times as potent as noradrenaline on the rat vas deferens, but was considerably less potent than noradrenaline on the guinea-pig vas deferens. 3. Phentolamine antagonized responses to noradrenaline and dopamine of vasa deferentia of both species. On the rat vas deferens the pA2 values against noradrenaline and dopamine were 7.6 and 7.4, respectively. 4. Pimozide, a dopamine antagonist, antagonized responses to dopamine on vasa deferentia of both species, but was not significantly active in antagonizing responses to noradrenaline. On the rat vas deferens, the pA2 value against dopamine was 7.9. t. The beta-adrenoceptor blocking agents, propranolol and oxprenolol slightly enhanced dopamine-induced contractions on vasa deferentia of both species. 6. It is suggested that dopamine might act on both a-adrenoceptors and dopaminoceptors in the vas deferens, and that the component due to activation of dopaminoceptors is greater in the rat than the guinea-pig."}
{"id": "PMID:466870", "title": "Liver alcohol and aldehyde dehydrogenase: inhibition and potentiation by histamine agonists and antagonists.", "content": "1. The in vitro effects of histamine, some other H1- and H2-receptor agonists and some antagonists were studied on the specific activities and kinetics of rat liver alcohol dehydrogenase (ADH), and cytoplasmic and mitochondrial liver aldehyde dehydrogenase (ALDH). 2. Histamine (H1- and H2-agonist) non-competitively inhibited ADH. There were no changes of cytoplasmic and mitochondrial liver ALDH activities in the presence of 2-(2-aminoethyl) pyridine. 3. Betazole (H2-receptor agonist) produced a competitive inhibition of mitochondrial ALDH but not of ADH or cytoplasmic ALDH. 4. Diphenhydramine (H1-receptor antagonist) non-competitively inhibited ADH at a lower concentration. It stimulated mitochondrial ALDH activity without changes in cytoplasmic ALDH from control values. 5. Burimamide (H2-receptor antagonist) produced a biphasic and dose-dependent stimulation and non-competitive inhibition of ADH and it non-competitively inhibited ALDH in both cytoplasmic and mitochondrial fractions. Metiamide (H2-receptor antagonist) non-competitively inhibited all ADH and ALDH of both liver fraction studied. 6. It is concluded that liver ADH and ALDH activity can be altered by compounds which affect both H1- and H2-histamine receptors and that these compounds may cause an in vivo potentiation and/or reduction of the toxic effect of ethanol.", "contents": "Liver alcohol and aldehyde dehydrogenase: inhibition and potentiation by histamine agonists and antagonists. 1. The in vitro effects of histamine, some other H1- and H2-receptor agonists and some antagonists were studied on the specific activities and kinetics of rat liver alcohol dehydrogenase (ADH), and cytoplasmic and mitochondrial liver aldehyde dehydrogenase (ALDH). 2. Histamine (H1- and H2-agonist) non-competitively inhibited ADH. There were no changes of cytoplasmic and mitochondrial liver ALDH activities in the presence of 2-(2-aminoethyl) pyridine. 3. Betazole (H2-receptor agonist) produced a competitive inhibition of mitochondrial ALDH but not of ADH or cytoplasmic ALDH. 4. Diphenhydramine (H1-receptor antagonist) non-competitively inhibited ADH at a lower concentration. It stimulated mitochondrial ALDH activity without changes in cytoplasmic ALDH from control values. 5. Burimamide (H2-receptor antagonist) produced a biphasic and dose-dependent stimulation and non-competitive inhibition of ADH and it non-competitively inhibited ALDH in both cytoplasmic and mitochondrial fractions. Metiamide (H2-receptor antagonist) non-competitively inhibited all ADH and ALDH of both liver fraction studied. 6. It is concluded that liver ADH and ALDH activity can be altered by compounds which affect both H1- and H2-histamine receptors and that these compounds may cause an in vivo potentiation and/or reduction of the toxic effect of ethanol."}
{"id": "PMID:466871", "title": "The effect of delta 9-tetrahydrocannabinol (delta 9-THC) on the turnover rate of brain serotonin of the rat.", "content": "1. One isotopic and three non-isotopic methods were used to determine the effect of an acute intravenous dose of delta 9-tetrahydrocannabinol (delta 9-THC, 2 mg/kg) on the rat brain turnover rate of serotonin. 2. In control animals the turnover rate of serotonin was about 2 nmol/g per h. This rate was not altered by delta 9-THC when it was calculated from the rise of 5-hydroxyindoleacetic acid following probenecid from the rise of serotonin following pargyline. 3. delta 9-THC did not alter serotonin turnover rate when it was calculated from the conversion of 3H-tryptophan to 3H-serotonin. 4. The serotonin turnover rate was significantly increased by delta 9-THC when the rate was calculated from the decline of 5-hydroxyindoleacetic acid following pargyline. 5. These results suggest that delta 9-THC does not alter the turnover of rat brain serotonin. The previously reported delta 9-THC-induced changes in body temperature and increased brain levels of 5-hydroxyindoleacetic acid may be mediated by some other mechanism such as interference by delta 9-THC of the vesicular binding of serotonin.", "contents": "The effect of delta 9-tetrahydrocannabinol (delta 9-THC) on the turnover rate of brain serotonin of the rat. 1. One isotopic and three non-isotopic methods were used to determine the effect of an acute intravenous dose of delta 9-tetrahydrocannabinol (delta 9-THC, 2 mg/kg) on the rat brain turnover rate of serotonin. 2. In control animals the turnover rate of serotonin was about 2 nmol/g per h. This rate was not altered by delta 9-THC when it was calculated from the rise of 5-hydroxyindoleacetic acid following probenecid from the rise of serotonin following pargyline. 3. delta 9-THC did not alter serotonin turnover rate when it was calculated from the conversion of 3H-tryptophan to 3H-serotonin. 4. The serotonin turnover rate was significantly increased by delta 9-THC when the rate was calculated from the decline of 5-hydroxyindoleacetic acid following pargyline. 5. These results suggest that delta 9-THC does not alter the turnover of rat brain serotonin. The previously reported delta 9-THC-induced changes in body temperature and increased brain levels of 5-hydroxyindoleacetic acid may be mediated by some other mechanism such as interference by delta 9-THC of the vesicular binding of serotonin."}
{"id": "PMID:466879", "title": "Adult bacteremia caused by gram-negative bacilli.", "content": "Because gram-negative bacteremia is increasing in frequency, it is important for physicians to suspect, carefully evaluate, and initiate early appropriate therapy. Identification of the probable source of bacteremia and a knowledge of the hospital microflora are helpful in guiding antibiotic selection. The mortality of gram-negative bacteremia tends to vary with the severity of the underlying disease and, accordingly, tends to be low in obstetrical patients. Prevention is the most effective method of controlling these infections and, therefore, care and discretion when employing invasive devices is essential. With the increasing incidence of these infections and the increased threat of antibiotic plasmid resistance, future infection control in high-risk patients by immunoprophylaxis may be necessary.", "contents": "Adult bacteremia caused by gram-negative bacilli. Because gram-negative bacteremia is increasing in frequency, it is important for physicians to suspect, carefully evaluate, and initiate early appropriate therapy. Identification of the probable source of bacteremia and a knowledge of the hospital microflora are helpful in guiding antibiotic selection. The mortality of gram-negative bacteremia tends to vary with the severity of the underlying disease and, accordingly, tends to be low in obstetrical patients. Prevention is the most effective method of controlling these infections and, therefore, care and discretion when employing invasive devices is essential. With the increasing incidence of these infections and the increased threat of antibiotic plasmid resistance, future infection control in high-risk patients by immunoprophylaxis may be necessary."}
{"id": "PMID:466888", "title": "Ambulatory evaluation and treatment of infertile couples.", "content": "With the exception of major surgical procedures, all investigative and therapeutic regimes regarding infertility can be instituted and carried to completion via the ambulatory office and hospital route if a foundation for understanding and cooperation between the physician and the couple is established during the initial stages.", "contents": "Ambulatory evaluation and treatment of infertile couples. With the exception of major surgical procedures, all investigative and therapeutic regimes regarding infertility can be instituted and carried to completion via the ambulatory office and hospital route if a foundation for understanding and cooperation between the physician and the couple is established during the initial stages."}
{"id": "PMID:466899", "title": "The scintigraphic visualization of a lingual thyroid after excision of associated ectopic thyroid tissue.", "content": "The scintigraphic findings in a 2-year-old boy with both a lingual thyroid and ectopic thyroid tissue in the neck are presented. One day following excision of the neck mass, no functioning thyroid tissue was seen. However, one month later, the lingual gland was well visualized. The possible reason for this is discussed.", "contents": "The scintigraphic visualization of a lingual thyroid after excision of associated ectopic thyroid tissue. The scintigraphic findings in a 2-year-old boy with both a lingual thyroid and ectopic thyroid tissue in the neck are presented. One day following excision of the neck mass, no functioning thyroid tissue was seen. However, one month later, the lingual gland was well visualized. The possible reason for this is discussed."}
{"id": "PMID:466900", "title": "Assessment of left ventricular function in aortic insufficiency using echocardiography, gated scintigraphy and contrast angiography.", "content": "Echocardiography, gated scintigraphy and contrast angiography were used to measure left ventricular ejection fractions in 20 patients with varying degrees of aortic insufficiency. There was good correlation between the radionuclide and the angiographic ejection fractions. Echocardiographic ejection fraction correlated less well with the angiographic ejection fraction. The radionuclide left ventricular ejection fraction may prove valuable in the noninvasive serial evaluation of left ventricular function in patients with chronic aortic insufficiency.", "contents": "Assessment of left ventricular function in aortic insufficiency using echocardiography, gated scintigraphy and contrast angiography. Echocardiography, gated scintigraphy and contrast angiography were used to measure left ventricular ejection fractions in 20 patients with varying degrees of aortic insufficiency. There was good correlation between the radionuclide and the angiographic ejection fractions. Echocardiographic ejection fraction correlated less well with the angiographic ejection fraction. The radionuclide left ventricular ejection fraction may prove valuable in the noninvasive serial evaluation of left ventricular function in patients with chronic aortic insufficiency."}
{"id": "PMID:466901", "title": "Rapid redistribution of thallium-201 post stress testing in a patient with variant angina.", "content": "Reperfusion of an ischemic area after stress scanning with 201TI can occur rapidly and early scanning post stress testing is emphasized.", "contents": "Rapid redistribution of thallium-201 post stress testing in a patient with variant angina. Reperfusion of an ischemic area after stress scanning with 201TI can occur rapidly and early scanning post stress testing is emphasized."}
{"id": "PMID:466902", "title": "Radionuclide Meckelogram with particular reference to false-positive results.", "content": "Abdominal imaging 99mTc-pertechnetate is a most sensitive and noninvasive procedure for detecting Meckels diverticulum. However, false-positive results are unavoidable. We report two in which false-positive results were obtained and present a summary of reported false-positive results.", "contents": "Radionuclide Meckelogram with particular reference to false-positive results. Abdominal imaging 99mTc-pertechnetate is a most sensitive and noninvasive procedure for detecting Meckels diverticulum. However, false-positive results are unavoidable. We report two in which false-positive results were obtained and present a summary of reported false-positive results."}
{"id": "PMID:466903", "title": "Diagnostic features of a unilateral fused kidney with a lower-pole inflamed, hemorrhagic cyst.", "content": "Numerous imaging procedures contributed to the diagnosis of unilateral fused kidney with an associated lower-pole mass in a 68-year old man with back and right lower quadrant pain. 99mTc-DMSA renal scintigraphy provided the best global picture of the renal anomaly and a lower-pole contour defect suggesting a mass.", "contents": "Diagnostic features of a unilateral fused kidney with a lower-pole inflamed, hemorrhagic cyst. Numerous imaging procedures contributed to the diagnosis of unilateral fused kidney with an associated lower-pole mass in a 68-year old man with back and right lower quadrant pain. 99mTc-DMSA renal scintigraphy provided the best global picture of the renal anomaly and a lower-pole contour defect suggesting a mass."}
{"id": "PMID:466904", "title": "False-positive 111In-labeled leukocyte scan in cystic fibrosis.", "content": "A false-positive 111In-labeled leukocyte scan was obtained in a girl with cystic fibrosis and infection. There was focal accumulation of 111In activity in the abdomen and marked pulmonary uptake. Surgical exploration and postmortem examination revealed no abdominal abnormality. The abnormal activity was apparently due to swallowed sputum which contained labeled leukocytes. Broader clinical implications are discussed.", "contents": "False-positive 111In-labeled leukocyte scan in cystic fibrosis. A false-positive 111In-labeled leukocyte scan was obtained in a girl with cystic fibrosis and infection. There was focal accumulation of 111In activity in the abdomen and marked pulmonary uptake. Surgical exploration and postmortem examination revealed no abdominal abnormality. The abnormal activity was apparently due to swallowed sputum which contained labeled leukocytes. Broader clinical implications are discussed."}
{"id": "PMID:466905", "title": "99mTc-sodium pertechnetate permeation into cerebrospinal fluid.", "content": "The integrity of the blood-brain barrier may be reflected by the blood and cerebrospinal fluid (CSF) if these two compartments are sampled at an appropriate interval after the administration of certain substances. After reading through the controversial literature, this study was undertaken to determine the frequency with which 99mTc-sodium pertechnetate entered the CSF to an abnormal extent, and to see whether this was related to any particular pathology. The plasma-to-CSF ratios were determined in 51 patients 4 hours following the intravenous administration of 0.5-10 mCi 99mTc-sodium pertechnetate. In 23 patients with active CNS tuberculosis, the mean value of the pertechnetate plasma-to-CSF ration was 32.12. In contradistinction, the plasma-to-CSF pertechnetate in 28 nontuberculous subjects was considerably higher (144.63). For the purpose of correlation, a 82Br partition test was also done on each subject 48 hours following the oral administration of NH82Br. The 48-hour study in each of these cases was generally in agreement with the pertechnetate studies: the plasma-to-CSF ratio mean value was 1.25 in patients with active CNS tuberculosis, while for the nontuberculous patients it was 3.32.", "contents": "99mTc-sodium pertechnetate permeation into cerebrospinal fluid. The integrity of the blood-brain barrier may be reflected by the blood and cerebrospinal fluid (CSF) if these two compartments are sampled at an appropriate interval after the administration of certain substances. After reading through the controversial literature, this study was undertaken to determine the frequency with which 99mTc-sodium pertechnetate entered the CSF to an abnormal extent, and to see whether this was related to any particular pathology. The plasma-to-CSF ratios were determined in 51 patients 4 hours following the intravenous administration of 0.5-10 mCi 99mTc-sodium pertechnetate. In 23 patients with active CNS tuberculosis, the mean value of the pertechnetate plasma-to-CSF ration was 32.12. In contradistinction, the plasma-to-CSF pertechnetate in 28 nontuberculous subjects was considerably higher (144.63). For the purpose of correlation, a 82Br partition test was also done on each subject 48 hours following the oral administration of NH82Br. The 48-hour study in each of these cases was generally in agreement with the pertechnetate studies: the plasma-to-CSF ratio mean value was 1.25 in patients with active CNS tuberculosis, while for the nontuberculous patients it was 3.32."}
{"id": "PMID:466906", "title": "Bone marrow scintigraphy in the evaluation of acute nonlymphocytic leukemia.", "content": "The clinical usefulness of bone marrow scintigraphy in evaluating adult acute nonlymphocytic leukemia (ANLL) was assessed through 39 studies of 23 adult patients. Nineteen patients were in various stages of relapse and remission of ANLL and four patients were preleukemic. Results of scintigraphic studies were correlated with information concerning bone marrow status, therapy, and subsequent clinical response. When 99mTc-sulfur colloid was utilized, eight of 34 studies were normal, but in the majority of patients there was an abnormal distribution of bone marrow reticuloendothial activity. Three of the patients with normal studies were in complete remission at the time of the normal scan. The duration of these remissions tended to be long. In four patients, 67Ga-citrate scintigraphy showed a wide variation of bone marrow uptake despite a marked increase in bone marrow myeloblasts. There was no significant correlation between scintigraphic distribution and bone marrow morphological status nor with subsequent ability to induce a complete remission with chemotherapy. Nevertheless, these studies raise the possibility that bone marrow scintigraphy may be useful in predicting the stability of an induced complete remission in ANLL. Confirmation of this possibility must await further studies.", "contents": "Bone marrow scintigraphy in the evaluation of acute nonlymphocytic leukemia. The clinical usefulness of bone marrow scintigraphy in evaluating adult acute nonlymphocytic leukemia (ANLL) was assessed through 39 studies of 23 adult patients. Nineteen patients were in various stages of relapse and remission of ANLL and four patients were preleukemic. Results of scintigraphic studies were correlated with information concerning bone marrow status, therapy, and subsequent clinical response. When 99mTc-sulfur colloid was utilized, eight of 34 studies were normal, but in the majority of patients there was an abnormal distribution of bone marrow reticuloendothial activity. Three of the patients with normal studies were in complete remission at the time of the normal scan. The duration of these remissions tended to be long. In four patients, 67Ga-citrate scintigraphy showed a wide variation of bone marrow uptake despite a marked increase in bone marrow myeloblasts. There was no significant correlation between scintigraphic distribution and bone marrow morphological status nor with subsequent ability to induce a complete remission with chemotherapy. Nevertheless, these studies raise the possibility that bone marrow scintigraphy may be useful in predicting the stability of an induced complete remission in ANLL. Confirmation of this possibility must await further studies."}
{"id": "PMID:466912", "title": "Plasmacytoma simulating meningioma on brain scan.", "content": "The flow study and static images of the brain scans of two patients with plasmacytoma of the skull were identical to the pattern most commonly seen with meningioma. There was a prominent blush on dynamic imaging which did not wash out combined with an intense uptake on static views adjacent to a meningeal surface. In patients with known multiple myeloma, the diagnosis of plasmacytoma of the skull or cranial cavity should be considered when this characteristic pattern is seen.", "contents": "Plasmacytoma simulating meningioma on brain scan. The flow study and static images of the brain scans of two patients with plasmacytoma of the skull were identical to the pattern most commonly seen with meningioma. There was a prominent blush on dynamic imaging which did not wash out combined with an intense uptake on static views adjacent to a meningeal surface. In patients with known multiple myeloma, the diagnosis of plasmacytoma of the skull or cranial cavity should be considered when this characteristic pattern is seen."}
{"id": "PMID:466913", "title": "Radiophosphate imaging of regional migratory osteoporosis.", "content": "A patient with regional migratory osteoporosis had somewhat unusual features in that there was involvement of the small joints of the foot demonstrable by 99mTc-methylene diphosphonate joint imaging.", "contents": "Radiophosphate imaging of regional migratory osteoporosis. A patient with regional migratory osteoporosis had somewhat unusual features in that there was involvement of the small joints of the foot demonstrable by 99mTc-methylene diphosphonate joint imaging."}
{"id": "PMID:466914", "title": "Usefulness of 99mTc-methylene diphosphonate scintigraphy in nephrocalcinosis.", "content": "Abnormally increased uptake of 99mTc-methylene diphosphonate in the kidney parenchyma was found in a patient with acutely exacerbated sarcoidosis. The findings could be related to active sites of nephrocalcinosis.", "contents": "Usefulness of 99mTc-methylene diphosphonate scintigraphy in nephrocalcinosis. Abnormally increased uptake of 99mTc-methylene diphosphonate in the kidney parenchyma was found in a patient with acutely exacerbated sarcoidosis. The findings could be related to active sites of nephrocalcinosis."}
{"id": "PMID:466918", "title": "Radionuclide computerized ventilation studies.", "content": "Studies of regional lung function have developed rapidly in the last few years. Xenon -133 is the most frequently used isotope because of its physiocochemical and biologic properties. Fixed counters are being replaced by the scintillation camera interfaced to a computer allowing easy acquisition and interpretation of results and numerical data. The use of isotopes other than 133Xe, such as 81mKr, is less widespread. In this article, a method is described for studying regional ventilation in which the isotope used is 133Xe, the radioactivity is recorded by a scintillation camera, interfaced to a computer andsix (or 12) regions for both lungs are selected and investigated. The regional ventilation is expressed as an absolute value in ml/min/ml of ventilated volume as well as the relative contribution in percent of each zone to the total ventilation and volume. To illustrate this method, the results are reported for healthy subjects and for patients with chronic bronchitis and emphysema. Scintigraphs of individual cases are presented. Certain theoretic problems related to calculation of regional ventilation are discussed.", "contents": "Radionuclide computerized ventilation studies. Studies of regional lung function have developed rapidly in the last few years. Xenon -133 is the most frequently used isotope because of its physiocochemical and biologic properties. Fixed counters are being replaced by the scintillation camera interfaced to a computer allowing easy acquisition and interpretation of results and numerical data. The use of isotopes other than 133Xe, such as 81mKr, is less widespread. In this article, a method is described for studying regional ventilation in which the isotope used is 133Xe, the radioactivity is recorded by a scintillation camera, interfaced to a computer andsix (or 12) regions for both lungs are selected and investigated. The regional ventilation is expressed as an absolute value in ml/min/ml of ventilated volume as well as the relative contribution in percent of each zone to the total ventilation and volume. To illustrate this method, the results are reported for healthy subjects and for patients with chronic bronchitis and emphysema. Scintigraphs of individual cases are presented. Certain theoretic problems related to calculation of regional ventilation are discussed."}
{"id": "PMID:466921", "title": "Behavior modification therapy in hyperactive children. Research and clinical implications.", "content": "One hundred fifty-seven studies employing behavior modification in the management of hyperactive and disruptive children were reviewed. The studies were analyzed against standards of scientific validity. The review found: (1) behavior modification was effective in alleviating problem behaviors; (2) token programs were the most commonly used; (3) both positive reinforcement and punishment were effective; positive reinforcement, however, had the advantage of improving self-esteem; (4) behavioral problems occurring in the home most likely require a home-based program; (5) behavior modification and stimulant medication can be used simultaneously, often with additive effects; and (6) long-term benefits beyond one year have not been assessed.", "contents": "Behavior modification therapy in hyperactive children. Research and clinical implications. One hundred fifty-seven studies employing behavior modification in the management of hyperactive and disruptive children were reviewed. The studies were analyzed against standards of scientific validity. The review found: (1) behavior modification was effective in alleviating problem behaviors; (2) token programs were the most commonly used; (3) both positive reinforcement and punishment were effective; positive reinforcement, however, had the advantage of improving self-esteem; (4) behavioral problems occurring in the home most likely require a home-based program; (5) behavior modification and stimulant medication can be used simultaneously, often with additive effects; and (6) long-term benefits beyond one year have not been assessed."}
{"id": "PMID:466923", "title": "Forecasting individual pharmacokinetics.", "content": "Often drug dosage may be chosen rationally by use of plasma concentration (CP) as the \"therapeutic\" end point. The ability to accurately forecast the CP resulting from a dosage regimen is central to choosing that regimen. Tradionally forecasting has been attempted only by accounting for known influences on pharmacokinetics, such as sex, age, and renal disease. One must also adjust for previously observed CPs. Herein, we discuss and explain an approach to both of these tasks, mainly focusing on the latter. The approach balances observed outcomes against prior expectations taking account of observation CP error. For digoxin, use of 1 measured CP, as opposed to none, improves forecast precision for future CPs by 40% (decrement in variance of forecast error), and 2 CPs improve it by 67%. There is also an increase in forecast accuracy (decrement in mean of forecast error) as the number of CPs used increases. After only 2, forecast accuracy and precision are as good as theoretically possible. Moreover, information from CPs is far more valuable for forecasting than that from observable patient features-sex, age, and the like; use of all the latter information does not improve accuracy and precision as much as only 1 CP.", "contents": "Forecasting individual pharmacokinetics. Often drug dosage may be chosen rationally by use of plasma concentration (CP) as the \"therapeutic\" end point. The ability to accurately forecast the CP resulting from a dosage regimen is central to choosing that regimen. Tradionally forecasting has been attempted only by accounting for known influences on pharmacokinetics, such as sex, age, and renal disease. One must also adjust for previously observed CPs. Herein, we discuss and explain an approach to both of these tasks, mainly focusing on the latter. The approach balances observed outcomes against prior expectations taking account of observation CP error. For digoxin, use of 1 measured CP, as opposed to none, improves forecast precision for future CPs by 40% (decrement in variance of forecast error), and 2 CPs improve it by 67%. There is also an increase in forecast accuracy (decrement in mean of forecast error) as the number of CPs used increases. After only 2, forecast accuracy and precision are as good as theoretically possible. Moreover, information from CPs is far more valuable for forecasting than that from observable patient features-sex, age, and the like; use of all the latter information does not improve accuracy and precision as much as only 1 CP."}
{"id": "PMID:466924", "title": "Plasma levels and renal excretion of phenytoin and its metabolites in patients with renal failure.", "content": "Phenytoin (DPH) and its two major metabolites, conjugated and unconjugated 5-(4-hydroxyphenyl)-5-phenylhydantoin (4-OH-DPH), have been studied in plasma and urine to 4 healthy subjects and 3 uremic patients during two weeks on DPH, 0.1 gm daily. Only 0.4% to 1.2% of the dose was excreted as unchanged DPH. The DPH concentrations in urine were in the same range as calculated unbound levels of DPH in plasma in the normal subjects; 1% to 2% of the dose was excreted as unconjugated 4-OH-DPH in the normal subjects. In the uremic patients, renal clearance of this metabolite was reduced to one-sixth that percentage. Plasma concentrations rose to values twice as high as normal, indicating increased rate of glucuronidation. Urinary recovery of conjugated 4-OH-DPH in healthy subjects was 52% to 94%. Its renal clearance was close to glomerular filtration rate when corrected for protein binding, suggesting elimination by glomerular filtration rate when corrected for protein binding, suggesting elimination by glomerular filtration only. Plasma concentrations of conjugated 4-OH-DPH reached plateau levels around day 4 in normal subjects. In the uremic patients, plasma concentrations of this metabolite accumulated to levels 10 times normal, and after 15 days of medication plateau levels did not seem to have been reached.", "contents": "Plasma levels and renal excretion of phenytoin and its metabolites in patients with renal failure. Phenytoin (DPH) and its two major metabolites, conjugated and unconjugated 5-(4-hydroxyphenyl)-5-phenylhydantoin (4-OH-DPH), have been studied in plasma and urine to 4 healthy subjects and 3 uremic patients during two weeks on DPH, 0.1 gm daily. Only 0.4% to 1.2% of the dose was excreted as unchanged DPH. The DPH concentrations in urine were in the same range as calculated unbound levels of DPH in plasma in the normal subjects; 1% to 2% of the dose was excreted as unconjugated 4-OH-DPH in the normal subjects. In the uremic patients, renal clearance of this metabolite was reduced to one-sixth that percentage. Plasma concentrations rose to values twice as high as normal, indicating increased rate of glucuronidation. Urinary recovery of conjugated 4-OH-DPH in healthy subjects was 52% to 94%. Its renal clearance was close to glomerular filtration rate when corrected for protein binding, suggesting elimination by glomerular filtration rate when corrected for protein binding, suggesting elimination by glomerular filtration only. Plasma concentrations of conjugated 4-OH-DPH reached plateau levels around day 4 in normal subjects. In the uremic patients, plasma concentrations of this metabolite accumulated to levels 10 times normal, and after 15 days of medication plateau levels did not seem to have been reached."}
{"id": "PMID:466926", "title": "Influence of pentobarbital on metoprolol plasma levels.", "content": "Induction of microsomal enzymes with barbiturates in rats has little effect on the metabolism of metoprolol, compared with propranolol and alprenolol, which undergo extensive hepatic extraction in animals and man. Our study was designed to examine whether the metabolism of metoprolol is inducable by barbiturate in man. In 8 healthy subjects the area under the plasma concentration/time curve after 0.1 gm metoprolol was reduced by a mean of 32% after treatment with 0.1 gm pentobarbital at bedtime for 10 days. There was considerable interindividual variability in the reduction after pentobarbital treatment (2% to 46%).", "contents": "Influence of pentobarbital on metoprolol plasma levels. Induction of microsomal enzymes with barbiturates in rats has little effect on the metabolism of metoprolol, compared with propranolol and alprenolol, which undergo extensive hepatic extraction in animals and man. Our study was designed to examine whether the metabolism of metoprolol is inducable by barbiturate in man. In 8 healthy subjects the area under the plasma concentration/time curve after 0.1 gm metoprolol was reduced by a mean of 32% after treatment with 0.1 gm pentobarbital at bedtime for 10 days. There was considerable interindividual variability in the reduction after pentobarbital treatment (2% to 46%)."}
{"id": "PMID:466927", "title": "Hemodynamic effects of intravenous timolol in coronary artery disease.", "content": "The hemodynamic effects of intravenous timolol were evaluated in 20 patients with coronary artery disease during diagnostic cardiac catheterization. The threshold dose of 0.25 mg reduced heart rate and cardiac index by 15% (p less than 0.05), left ventricular work index by 21% (p less than 0.05), and left ventricular dp/dt by 16% (p less than 0.05) while increasing left ventricular end-diastolic pressure by 49% (p less than 0.01), mean pulmonary arterial pressure by 17% (p less than 0.01), and systemic vascular resistance by 16% (NS). Larger doses (0.5 mg and 1.0 mg) induced similar responses with a greater effect on systemic vascular resistance (+22%, p less than 0.01, and +31%, p less than 0.001). The mean arterial pressure and stroke volumes were not affected by timolol. Peak effects, occurring at about 10 min after drug injection, did not correlate with plasma levels. The overall hemodynamic effects of timolol were similar to those reported for equipotent doses of propranolol and could be accounted for by the beta-adrenoceptor blocking activity.", "contents": "Hemodynamic effects of intravenous timolol in coronary artery disease. The hemodynamic effects of intravenous timolol were evaluated in 20 patients with coronary artery disease during diagnostic cardiac catheterization. The threshold dose of 0.25 mg reduced heart rate and cardiac index by 15% (p less than 0.05), left ventricular work index by 21% (p less than 0.05), and left ventricular dp/dt by 16% (p less than 0.05) while increasing left ventricular end-diastolic pressure by 49% (p less than 0.01), mean pulmonary arterial pressure by 17% (p less than 0.01), and systemic vascular resistance by 16% (NS). Larger doses (0.5 mg and 1.0 mg) induced similar responses with a greater effect on systemic vascular resistance (+22%, p less than 0.01, and +31%, p less than 0.001). The mean arterial pressure and stroke volumes were not affected by timolol. Peak effects, occurring at about 10 min after drug injection, did not correlate with plasma levels. The overall hemodynamic effects of timolol were similar to those reported for equipotent doses of propranolol and could be accounted for by the beta-adrenoceptor blocking activity."}
{"id": "PMID:466928", "title": "Morphine kinetics in children.", "content": "The kinetics of morphine in the plasma of children (0 to 15 yr) can in most cases be adequately described by a triexponential function. When the dosage in children receiving morphine as premedication before surgery is based on kilograms of body weight, there are only minor differences in the kinetic patterns of morphine at different ages (0 to 1, 1 to 7, and 7 to 15 yr). No significant difference in the morphine kinetics was observed between patients (7 to 15 yr) receiving the drug only as premedication and those who also received doses during surgery. The minimum morphine concentration in plasma necessary to suppress the clinical signs of pain during surgery was found to be 65 ng/ml (95% confidence limits of 46 to 83 ng/ml). Based on these minimum effective plasma levels of morphine, there does not seem to be any difference between children of different ages in their sensitivity to morphine. No difference was found in the minimum effective level of morphine when clinical signs of pain were observed by different anesthesiologists.", "contents": "Morphine kinetics in children. The kinetics of morphine in the plasma of children (0 to 15 yr) can in most cases be adequately described by a triexponential function. When the dosage in children receiving morphine as premedication before surgery is based on kilograms of body weight, there are only minor differences in the kinetic patterns of morphine at different ages (0 to 1, 1 to 7, and 7 to 15 yr). No significant difference in the morphine kinetics was observed between patients (7 to 15 yr) receiving the drug only as premedication and those who also received doses during surgery. The minimum morphine concentration in plasma necessary to suppress the clinical signs of pain during surgery was found to be 65 ng/ml (95% confidence limits of 46 to 83 ng/ml). Based on these minimum effective plasma levels of morphine, there does not seem to be any difference between children of different ages in their sensitivity to morphine. No difference was found in the minimum effective level of morphine when clinical signs of pain were observed by different anesthesiologists."}
{"id": "PMID:466929", "title": "Kinetics and metabolism of carbamazepine during combined antiepileptic drug therapy.", "content": "The kinetics of carbamazepine using 15N-carbamazepine were investigated in epileptic patients during combined anticonvulsant therapy. The 15N-carbamazepine plasma half-lives ranged from 5.0 to 13.6 hr with a mean of 8.2 hr. These half-lives are appreciably shorter than reported during chronic carbamazepine monotherapy. Predicted steady-state plasma levels and observed plasma levels of carbamazepine were in excellent agreement. Between 32% and 61% of the dose administered is excreted in the urine as carbamazepine-trans-diol, 5.2% to 8.8% as 9-hydroxymethyl-10-carbamoyl acridane, 1% to 1.4% as 10,-11-carbamazepine epoxide, and 0.5% as carbamazepine. The data indicate that it is the epoxide-diol pathway which is induced during long-term treatment. Concomitant therapy with primidone, phenytoin, phenobarbital, ethosuximide, or methsuximide further induces carbamazepine metabolism.", "contents": "Kinetics and metabolism of carbamazepine during combined antiepileptic drug therapy. The kinetics of carbamazepine using 15N-carbamazepine were investigated in epileptic patients during combined anticonvulsant therapy. The 15N-carbamazepine plasma half-lives ranged from 5.0 to 13.6 hr with a mean of 8.2 hr. These half-lives are appreciably shorter than reported during chronic carbamazepine monotherapy. Predicted steady-state plasma levels and observed plasma levels of carbamazepine were in excellent agreement. Between 32% and 61% of the dose administered is excreted in the urine as carbamazepine-trans-diol, 5.2% to 8.8% as 9-hydroxymethyl-10-carbamoyl acridane, 1% to 1.4% as 10,-11-carbamazepine epoxide, and 0.5% as carbamazepine. The data indicate that it is the epoxide-diol pathway which is induced during long-term treatment. Concomitant therapy with primidone, phenytoin, phenobarbital, ethosuximide, or methsuximide further induces carbamazepine metabolism."}
{"id": "PMID:466931", "title": "Salicylate clearance, the resultant of protein binding and metabolism.", "content": "Steady-state plasma salicylate concentrations and protein binding were examined in 9 normal subjects to determine relationships among daily dose, total and unbound salicylate concentrations, and total and unbound clearances. Aspirin doses ranging from 0.66 to 4.0 mg/kg/hr were given to steady state. Free and total salicylate concentrations were measured with spectrophotometric, fluorimetric, and equilibrium dialysis techniques. Although unbound clearance decreased over the therapeutic range, total clearance was unchanged. The former is a consequence of saturable metabolism; the latter, of saturable plasma protein binding as well as saturable metabolism. The fraction unbound increased linearly with unbound concentration. Clearance determined at 1.8 mg/kg/hr was used to predict levels obtained at higher aspirin doses. Analysis of residuals was used to ascertain the accuracy of the prediction. The coefficient of variation from prediction among subjects was found to be +/- 14%. It is concluded that, in normal subjects, salicylate clearance changes relatively little over the therapeutic range because the increasing fraction unbound compensates for decreasing clearance of unbound drug.", "contents": "Salicylate clearance, the resultant of protein binding and metabolism. Steady-state plasma salicylate concentrations and protein binding were examined in 9 normal subjects to determine relationships among daily dose, total and unbound salicylate concentrations, and total and unbound clearances. Aspirin doses ranging from 0.66 to 4.0 mg/kg/hr were given to steady state. Free and total salicylate concentrations were measured with spectrophotometric, fluorimetric, and equilibrium dialysis techniques. Although unbound clearance decreased over the therapeutic range, total clearance was unchanged. The former is a consequence of saturable metabolism; the latter, of saturable plasma protein binding as well as saturable metabolism. The fraction unbound increased linearly with unbound concentration. Clearance determined at 1.8 mg/kg/hr was used to predict levels obtained at higher aspirin doses. Analysis of residuals was used to ascertain the accuracy of the prediction. The coefficient of variation from prediction among subjects was found to be +/- 14%. It is concluded that, in normal subjects, salicylate clearance changes relatively little over the therapeutic range because the increasing fraction unbound compensates for decreasing clearance of unbound drug."}
{"id": "PMID:466932", "title": "Effect of thiazides on serum calcium.", "content": "A retrospective study of 22 hypertensive patients who were treated with thiazide diuretics for 2 to 12 yr revealed that 36% developed transient, self-limited asymptomatic elevations of serum calcium which occurred at varying periods of therapy and returned to normal within 2 to 4 wk despite continued administration of thiazides. These episodes of hypercalcemia correlated positively with increases in total protein, albumin, and globulin. The same phenomenon of intermittent hypercalcemia occurred in a prospective study of 11 patients but not in control subjects. The mean serum total calcium of the prospectively studied hydrochlorothiazide-treated patients was found to be higher than the nonthiazide control group. This difference was due to increased protein-bound calcium. The total proteins, serum albumin, and serum beta globulins of the treated group were higher, probably due to depletion of extracellular fluid. The presence of slightly elevated serum calcium in a patient treated with thiazides appears to be a common phenomenon and, unless it is marked, should not necessarily be construed as indicating covert hyperparathyroidism.", "contents": "Effect of thiazides on serum calcium. A retrospective study of 22 hypertensive patients who were treated with thiazide diuretics for 2 to 12 yr revealed that 36% developed transient, self-limited asymptomatic elevations of serum calcium which occurred at varying periods of therapy and returned to normal within 2 to 4 wk despite continued administration of thiazides. These episodes of hypercalcemia correlated positively with increases in total protein, albumin, and globulin. The same phenomenon of intermittent hypercalcemia occurred in a prospective study of 11 patients but not in control subjects. The mean serum total calcium of the prospectively studied hydrochlorothiazide-treated patients was found to be higher than the nonthiazide control group. This difference was due to increased protein-bound calcium. The total proteins, serum albumin, and serum beta globulins of the treated group were higher, probably due to depletion of extracellular fluid. The presence of slightly elevated serum calcium in a patient treated with thiazides appears to be a common phenomenon and, unless it is marked, should not necessarily be construed as indicating covert hyperparathyroidism."}
{"id": "PMID:466933", "title": "Diazepam prevents some adverse effects of succinylcholine.", "content": "Neuromuscular, circulatory, and adverse effects of intravenous succinylcholine (SCh), mg/kg, were compared in 3 groups of 40 patients each. Group I served as control; group II received diazepam, 0.05 mg/kg, 5 min before SCh; and group III was given d-tubocurarine (d-Tc), 0.05 mg/kg, for pretreatment. Diazepam pretreatment prevented muscle fasciculations, increases in serum potassium (K+) and creatinine phosphokinase (CPK) levels, increased heart rate and arterial pressure, and postoperative myalgia associated with SCh administration. The neuromuscular blocking action of SCh was not affected. Pretreatment with d-Tc did not abolish increases in serum K+ and CPK levels, was associated with a higher incidence of postoperative myalgia, and decreased the onset and magnitude of SCh-induced muscle paralysis. Our data demonstrate that diazepam, a predominant centrally acting muscle relaxant, is more effective than d-Tc in preventing the adverse effects of SCh, a peripherally acting muscle relaxant.", "contents": "Diazepam prevents some adverse effects of succinylcholine. Neuromuscular, circulatory, and adverse effects of intravenous succinylcholine (SCh), mg/kg, were compared in 3 groups of 40 patients each. Group I served as control; group II received diazepam, 0.05 mg/kg, 5 min before SCh; and group III was given d-tubocurarine (d-Tc), 0.05 mg/kg, for pretreatment. Diazepam pretreatment prevented muscle fasciculations, increases in serum potassium (K+) and creatinine phosphokinase (CPK) levels, increased heart rate and arterial pressure, and postoperative myalgia associated with SCh administration. The neuromuscular blocking action of SCh was not affected. Pretreatment with d-Tc did not abolish increases in serum K+ and CPK levels, was associated with a higher incidence of postoperative myalgia, and decreased the onset and magnitude of SCh-induced muscle paralysis. Our data demonstrate that diazepam, a predominant centrally acting muscle relaxant, is more effective than d-Tc in preventing the adverse effects of SCh, a peripherally acting muscle relaxant."}
{"id": "PMID:466937", "title": "Clinical trial design in cancer.", "content": "The design of clinical trials in cancer is a subject which features reasonably often among FRCR (Part 1) examination questions, and as such should be of more than passing interest to oncologists. It is also a subject which is gaining in relevance since the number of trials is increasing annually due in part to the many chemotherapeutic regimes which are being proposed. This paper which is based on a lecture given in Cambridge at the Hospital Physicists' Association Annual Conference in September 1978, is intended to act as an introduction to clinical trial design. References for further reading are given and, in particular, the extensive report on randomised clinical trials to the Medical Research Council's Leukaemia Steering Committee (Peto et al., 1977, 1978) is recommended.", "contents": "Clinical trial design in cancer. The design of clinical trials in cancer is a subject which features reasonably often among FRCR (Part 1) examination questions, and as such should be of more than passing interest to oncologists. It is also a subject which is gaining in relevance since the number of trials is increasing annually due in part to the many chemotherapeutic regimes which are being proposed. This paper which is based on a lecture given in Cambridge at the Hospital Physicists' Association Annual Conference in September 1978, is intended to act as an introduction to clinical trial design. References for further reading are given and, in particular, the extensive report on randomised clinical trials to the Medical Research Council's Leukaemia Steering Committee (Peto et al., 1977, 1978) is recommended."}
{"id": "PMID:466938", "title": "The role of computed tomography in the staging of bladder cancer.", "content": "Computed tomography of the pelvis has been performed in 60 patients with epithelial bladder tumours. The CT findings have been compared with the clinical staging (T-stage), lymphography (N-stage) and wherever possible the surgical staging (P-stage). Although the intraluminal tumour was visualised in a high proportion of examinations, the greatest value of CT is in the accurate delineation of the extravesical extension of the growth. This is likely to be the primary role of CT in the staging of bladder cancer. Difficulties in detecting invasion of contiguous organs, particularly the prostate, and the failure to demonstrate nodal involvement within the pelvis were noted. The technique has clear advantages over more invasive investigations and the additional information provided over and above clinical staging is seen as a major advance in the assessment of these tumours.", "contents": "The role of computed tomography in the staging of bladder cancer. Computed tomography of the pelvis has been performed in 60 patients with epithelial bladder tumours. The CT findings have been compared with the clinical staging (T-stage), lymphography (N-stage) and wherever possible the surgical staging (P-stage). Although the intraluminal tumour was visualised in a high proportion of examinations, the greatest value of CT is in the accurate delineation of the extravesical extension of the growth. This is likely to be the primary role of CT in the staging of bladder cancer. Difficulties in detecting invasion of contiguous organs, particularly the prostate, and the failure to demonstrate nodal involvement within the pelvis were noted. The technique has clear advantages over more invasive investigations and the additional information provided over and above clinical staging is seen as a major advance in the assessment of these tumours."}
{"id": "PMID:466939", "title": "Vacuum contoured, liquid crystal, dynamic breast thermoangiography as an aid to mammography in the detection of breast cancer.", "content": "New thermographic equipment is introduced in the form of liquid crystals embedded in flexible, transparent, elastomeric sheaths which may be vacuum contoured to any breast. Dynamic thermoangiography, a new physiological method of performing thermography with these liquid crystal sheaths, is presented. Thermographic changes are recorded during active skin cooling and rewarming with liquid crystals in situ. The new system eliminates skin preparation previously required. Preliminary results appear to show good correlation with conventional telethermography. Vascular, as well as thermal pathology, is accentuated with rich, high contrast, thermographically calibrated colour patterns. The new system is relatively inexpensive, simple to operate, and may be suited to a programme for the detection of breast cancer.", "contents": "Vacuum contoured, liquid crystal, dynamic breast thermoangiography as an aid to mammography in the detection of breast cancer. New thermographic equipment is introduced in the form of liquid crystals embedded in flexible, transparent, elastomeric sheaths which may be vacuum contoured to any breast. Dynamic thermoangiography, a new physiological method of performing thermography with these liquid crystal sheaths, is presented. Thermographic changes are recorded during active skin cooling and rewarming with liquid crystals in situ. The new system eliminates skin preparation previously required. Preliminary results appear to show good correlation with conventional telethermography. Vascular, as well as thermal pathology, is accentuated with rich, high contrast, thermographically calibrated colour patterns. The new system is relatively inexpensive, simple to operate, and may be suited to a programme for the detection of breast cancer."}
{"id": "PMID:466940", "title": "The chest X-ray in pulmonary capillary haemorrhage: correlation with carbon monoxide uptake.", "content": "Serial changes in carbon monoxide uptake (KCO or DLCO/VA) were used to monitor episodes of pulmonary capillary haemorrhage in Goodpasture's syndrome (13 cases), immune complex nephritis (three cases) and idiopathic haemosiderosis (one case). Air-space shadowing on the chest X-ray (on a scoring system 0 to 12) was assessed in the light of the changes of KCO. In 14 out of 27 episodes of bleeding, the rise and fall of KCO was matched in time by the appearance and disappearance of air-space shadowing on the chest X-ray. In six episodes the chest X-ray remained normal despite a rise of KCO. In two cases air-space shadowing appeared up to 48 h after the rise of KCO. On five occasions chest X-ray abnormalities preceded the rise of KCO but chest infection or fluid overload accounted for three of these. In cases with suspected pulmonary capillary haemorrhage, measurements of carbon monoxide uptake will provide additional information and will assist in the interpretation of the chest X-ray.", "contents": "The chest X-ray in pulmonary capillary haemorrhage: correlation with carbon monoxide uptake. Serial changes in carbon monoxide uptake (KCO or DLCO/VA) were used to monitor episodes of pulmonary capillary haemorrhage in Goodpasture's syndrome (13 cases), immune complex nephritis (three cases) and idiopathic haemosiderosis (one case). Air-space shadowing on the chest X-ray (on a scoring system 0 to 12) was assessed in the light of the changes of KCO. In 14 out of 27 episodes of bleeding, the rise and fall of KCO was matched in time by the appearance and disappearance of air-space shadowing on the chest X-ray. In six episodes the chest X-ray remained normal despite a rise of KCO. In two cases air-space shadowing appeared up to 48 h after the rise of KCO. On five occasions chest X-ray abnormalities preceded the rise of KCO but chest infection or fluid overload accounted for three of these. In cases with suspected pulmonary capillary haemorrhage, measurements of carbon monoxide uptake will provide additional information and will assist in the interpretation of the chest X-ray."}
{"id": "PMID:466941", "title": "The chest X-ray in antiglomerular basement membrane antibody disease (Goodpasture's syndrome).", "content": "The chest radiographs of 25 patients with proven antiglomerular basement membrane antibody disease (Goodpasture's syndrome) were analysed. All except two of the patients had pulmonary haemorrhage at some stage of their disease. Altogether there were 39 episodes of pulmonary haemorrhage, 25 being relapses. During seven episodes the chest radiograph was normal. Relapses of pulmonary haemorrhage never occurred in isolation but were usually associated with infection (not necessarily a chest infection) or occasionally fluid overload. Conversely fluid overload or infection were always associated with pulmonary haemorrhage provided there were high or rising titres of circulating antibodies at the time. Therefore in a patient with antiglomerular basement membrane antibody disease, the presence of shadowing in the lung fields on the chest radiograph almost invariably means the patient has pulmonary haemorrhage whether or not pulmonary oedema or a chest infection are present. Limitation of shadowing by a fissure, loss of major portions of the diaphragmatic or cardiac silhouette, involvement of the lung apex or costophrenic angles suggest an underlying chest infection. Septal lines suggest fluid overload. Pleural effusions are seen with chest infections and fluid overload. The carbon monoxide uptake (KCO) was invariably high in the presence of pulmonary haemorrhage even if the chest radiograph was normal. A combined use of KCO and chest radiographs is the best method of monitoring lung disease in these patients.", "contents": "The chest X-ray in antiglomerular basement membrane antibody disease (Goodpasture's syndrome). The chest radiographs of 25 patients with proven antiglomerular basement membrane antibody disease (Goodpasture's syndrome) were analysed. All except two of the patients had pulmonary haemorrhage at some stage of their disease. Altogether there were 39 episodes of pulmonary haemorrhage, 25 being relapses. During seven episodes the chest radiograph was normal. Relapses of pulmonary haemorrhage never occurred in isolation but were usually associated with infection (not necessarily a chest infection) or occasionally fluid overload. Conversely fluid overload or infection were always associated with pulmonary haemorrhage provided there were high or rising titres of circulating antibodies at the time. Therefore in a patient with antiglomerular basement membrane antibody disease, the presence of shadowing in the lung fields on the chest radiograph almost invariably means the patient has pulmonary haemorrhage whether or not pulmonary oedema or a chest infection are present. Limitation of shadowing by a fissure, loss of major portions of the diaphragmatic or cardiac silhouette, involvement of the lung apex or costophrenic angles suggest an underlying chest infection. Septal lines suggest fluid overload. Pleural effusions are seen with chest infections and fluid overload. The carbon monoxide uptake (KCO) was invariably high in the presence of pulmonary haemorrhage even if the chest radiograph was normal. A combined use of KCO and chest radiographs is the best method of monitoring lung disease in these patients."}
{"id": "PMID:466942", "title": "The computerised tomographic findings in paediatric orbital tumours.", "content": "Forty-two patients with histologically proven orbital tumours are presented. CT is of value in the pretreatment assessment and in delineating the extent of the orbital tumour. The surrounding bony destruction and intracranial extension are easily assessed. Post-treatment follow-up can be closely monitored using CT.", "contents": "The computerised tomographic findings in paediatric orbital tumours. Forty-two patients with histologically proven orbital tumours are presented. CT is of value in the pretreatment assessment and in delineating the extent of the orbital tumour. The surrounding bony destruction and intracranial extension are easily assessed. Post-treatment follow-up can be closely monitored using CT."}
{"id": "PMID:466944", "title": "The role of endoscopic retrograde cholangiography in the diagnosis and management of patients with primary sclerosing cholangitis.", "content": "The clinical and radiological findings in four patients with primary sclerosing cholangitis one of whom had coexistent cholangiocarcinoma, are reported. The need for surgical exploration to make the diagnosis was averted by the use of endoscopic retrograde cholangiography in one patient who was managed initially with medical treatment alone. Endoscopic cholangiography may be used to monitor the progress of the sclerosing lesions; but failure to fill the intrahepatic ducts is associated with a poor prognosis due either to the severity of the sclerosing process or the presence of coexistent cholangiocarcinoma.", "contents": "The role of endoscopic retrograde cholangiography in the diagnosis and management of patients with primary sclerosing cholangitis. The clinical and radiological findings in four patients with primary sclerosing cholangitis one of whom had coexistent cholangiocarcinoma, are reported. The need for surgical exploration to make the diagnosis was averted by the use of endoscopic retrograde cholangiography in one patient who was managed initially with medical treatment alone. Endoscopic cholangiography may be used to monitor the progress of the sclerosing lesions; but failure to fill the intrahepatic ducts is associated with a poor prognosis due either to the severity of the sclerosing process or the presence of coexistent cholangiocarcinoma."}
{"id": "PMID:466945", "title": "Diagnostic value of percutaneous transhepatic cholangiography judged by personal experience of 58 patients.", "content": "During the last seven years percutaneous transhepatic cholangiography (PTC) using a fine needle was performed in 58 patients. The biliary tract was visualised and opacified in the 52 patients with dilated biliary ducts. The correct diagnoses were made in 51 of them. In six patients with a non-dilated biliary system, successful PTC was carried out with the correct diagnoses in four of them. There were four non-fatal complications; two septic reactions, one haemobilia, and one subcapsular haematoma, but all were controlled by conservative treatment alone. The PTC with a fine needle is an accurate, useful and safe procedure of choice for the diagnosis of obstructive jaundice and jaundice of obscure origin. It is useful in distinguishing hepatocellular jaundice from obstructive jaundice thus preventing an unnecessary operation. Failure to visualise the biliary tract by an experienced hand strongly indicates that the jaundice is non-obstructive.", "contents": "Diagnostic value of percutaneous transhepatic cholangiography judged by personal experience of 58 patients. During the last seven years percutaneous transhepatic cholangiography (PTC) using a fine needle was performed in 58 patients. The biliary tract was visualised and opacified in the 52 patients with dilated biliary ducts. The correct diagnoses were made in 51 of them. In six patients with a non-dilated biliary system, successful PTC was carried out with the correct diagnoses in four of them. There were four non-fatal complications; two septic reactions, one haemobilia, and one subcapsular haematoma, but all were controlled by conservative treatment alone. The PTC with a fine needle is an accurate, useful and safe procedure of choice for the diagnosis of obstructive jaundice and jaundice of obscure origin. It is useful in distinguishing hepatocellular jaundice from obstructive jaundice thus preventing an unnecessary operation. Failure to visualise the biliary tract by an experienced hand strongly indicates that the jaundice is non-obstructive."}
{"id": "PMID:466947", "title": "Radiological findings of the urinary tract in hypospadias: a report of 110 cases.", "content": "One hundred and ten patients with hypospadias underwent excretory urography and micturating cysto-urethrography before surgical correction. Urographic studies revealed abnormalities in 50 patients (45%), mainly anomalies of ascent and rotation of the kidneys. None of these patients required corrective surgery. Voiding cysto-urethrography disclosed disease of the urinary tract in 65 of 110 patients (59%). Meatal stenosis was found in 38 patients and a rudimentary vagina or a prostatic utricle was revealed in 11 others. Vesico-ureteral reflux was present in 14 patients, seven of whom had meatal stenosis as well. Cystitis was found in eight and a stricture of the membraneous urethra in one patient. The voiding cysto-urethrogram is able to demonstrate functionally significant meatal stenosis, genital abnormalities and vesico-ureteral reflux. It can also serve as a useful baseline for comparison with postoperative studies. We therefore consider that it should be included in the preoperative work-up of every patient with hypospadias. Excretory urography, however, is perhaps indicated only in those with urinary tract infection, or in patients otherwise symptomatic.", "contents": "Radiological findings of the urinary tract in hypospadias: a report of 110 cases. One hundred and ten patients with hypospadias underwent excretory urography and micturating cysto-urethrography before surgical correction. Urographic studies revealed abnormalities in 50 patients (45%), mainly anomalies of ascent and rotation of the kidneys. None of these patients required corrective surgery. Voiding cysto-urethrography disclosed disease of the urinary tract in 65 of 110 patients (59%). Meatal stenosis was found in 38 patients and a rudimentary vagina or a prostatic utricle was revealed in 11 others. Vesico-ureteral reflux was present in 14 patients, seven of whom had meatal stenosis as well. Cystitis was found in eight and a stricture of the membraneous urethra in one patient. The voiding cysto-urethrogram is able to demonstrate functionally significant meatal stenosis, genital abnormalities and vesico-ureteral reflux. It can also serve as a useful baseline for comparison with postoperative studies. We therefore consider that it should be included in the preoperative work-up of every patient with hypospadias. Excretory urography, however, is perhaps indicated only in those with urinary tract infection, or in patients otherwise symptomatic."}
{"id": "PMID:466948", "title": "The radiological assessment of body fat.", "content": "A radiological method for assessment of obesity is described. A radiograph of the soft tissues of the upper arm is obtained and the subcutaneous fat layer measured. The results obtained by this method have been compared with those using a skinfold caliper and with the ponderal index, calculated from the height and weight of the subject. A highly significant correlation was found. The radiological method has several advantages: it leaves a permanent record, it is accurate in obese persons when the skinfold caliper cannot be used, and unlike the ponderal index it is not influenced by weight increases due to causes other than fat accumulation.", "contents": "The radiological assessment of body fat. A radiological method for assessment of obesity is described. A radiograph of the soft tissues of the upper arm is obtained and the subcutaneous fat layer measured. The results obtained by this method have been compared with those using a skinfold caliper and with the ponderal index, calculated from the height and weight of the subject. A highly significant correlation was found. The radiological method has several advantages: it leaves a permanent record, it is accurate in obese persons when the skinfold caliper cannot be used, and unlike the ponderal index it is not influenced by weight increases due to causes other than fat accumulation."}
{"id": "PMID:466950", "title": "Absence of oesophageal mucosal folds in systemic sclerosis.", "content": "In a controlled study of barium swallow radiographs in systemic sclerosis, longitudinal oesophageal mucosal folds were absent in eight of 30 patients and in one of 30 control subjects (P less than 0.03). Patients without mucosal folds developed Raynaud's phenomenon at an earlier age than those who retained their mucosal folds (P less than 0.05). A loss of oesophageal mucosal folds did not necessarily signify more severe visceral or cutaneous disease.", "contents": "Absence of oesophageal mucosal folds in systemic sclerosis. In a controlled study of barium swallow radiographs in systemic sclerosis, longitudinal oesophageal mucosal folds were absent in eight of 30 patients and in one of 30 control subjects (P less than 0.03). Patients without mucosal folds developed Raynaud's phenomenon at an earlier age than those who retained their mucosal folds (P less than 0.05). A loss of oesophageal mucosal folds did not necessarily signify more severe visceral or cutaneous disease."}
{"id": "PMID:466951", "title": "Perforated oesophagus: review of twenty-eight consecutive cases.", "content": "Oesophageal perforation is a potentially lethal condition which requires early diagnosis and prompt surgical management. The radiological features in 28 patients with oesophageal perforation have been reviewed and correlated with the aetiology and site of perforation, underlying oesophageal abnormality and the time interval between occurrence and diagnosis. Twenty-five patients (89%) had detectable abnormalities on the chest radiograph. The commonest abnormalities were air in the cervical or mediastinal soft tissues and air or fluid in the pleural cavity. The factors influencing the different radiographic signs are discussed. Studies with a suitable contrast medium are necessary to show the site and extent of the perforation, the extent of any associated abscess cavity and the position of the perforation relative to any primary oesophageal disease. Of 26 patients who had a barium swallow a leak was demonstrated in 21 (81%). In the five patients in whom no leak was demonstrated the examination was performed more than 24 h after the perforation.", "contents": "Perforated oesophagus: review of twenty-eight consecutive cases. Oesophageal perforation is a potentially lethal condition which requires early diagnosis and prompt surgical management. The radiological features in 28 patients with oesophageal perforation have been reviewed and correlated with the aetiology and site of perforation, underlying oesophageal abnormality and the time interval between occurrence and diagnosis. Twenty-five patients (89%) had detectable abnormalities on the chest radiograph. The commonest abnormalities were air in the cervical or mediastinal soft tissues and air or fluid in the pleural cavity. The factors influencing the different radiographic signs are discussed. Studies with a suitable contrast medium are necessary to show the site and extent of the perforation, the extent of any associated abscess cavity and the position of the perforation relative to any primary oesophageal disease. Of 26 patients who had a barium swallow a leak was demonstrated in 21 (81%). In the five patients in whom no leak was demonstrated the examination was performed more than 24 h after the perforation."}
{"id": "PMID:466952", "title": "The effect of glucagon on infusion cholangiography.", "content": "The study was undertaken to assess the effects of glucagon on biliary tract opacification during intravenous cholangiography. Two series of infusion cholangiograms were obtained at two investigating centres designated A and B. In series A, 41 patients had ioglycamide infusions at a rate of 0.2833 g min-1 over 1 h. In series B, 31 patients had ioglycamide infusions at a rate of 0.3886 g min-1 over 30 min. Radiographs were taken in both series, immediately at the end of the infusion, 10 min later and 30 min after the infusion. Two mg of intravenous glucagon was injected into alternate cases in both series A and B immediately after the first radiograph was taken at the completion of the ioglycamide infusion. Two observers in each series than assessed the radiographic opacification of the biliary system without prior knowledge of which patients had received the glucagon. Delineation of the biliary system was considered better in both series in those patients who received glucagon when compared with the controls. Gallbladder opacification was definitely increased in series A in those receiving glucagon, and a similar tendency was shown in series B. The amount of contrast in the upper intestine was increased in series A in the glucagon group, but not in series B. It is concluded that glucagon improves visualisation of the biliary tract, especially the gallbladder at infusion cholangiography.", "contents": "The effect of glucagon on infusion cholangiography. The study was undertaken to assess the effects of glucagon on biliary tract opacification during intravenous cholangiography. Two series of infusion cholangiograms were obtained at two investigating centres designated A and B. In series A, 41 patients had ioglycamide infusions at a rate of 0.2833 g min-1 over 1 h. In series B, 31 patients had ioglycamide infusions at a rate of 0.3886 g min-1 over 30 min. Radiographs were taken in both series, immediately at the end of the infusion, 10 min later and 30 min after the infusion. Two mg of intravenous glucagon was injected into alternate cases in both series A and B immediately after the first radiograph was taken at the completion of the ioglycamide infusion. Two observers in each series than assessed the radiographic opacification of the biliary system without prior knowledge of which patients had received the glucagon. Delineation of the biliary system was considered better in both series in those patients who received glucagon when compared with the controls. Gallbladder opacification was definitely increased in series A in those receiving glucagon, and a similar tendency was shown in series B. The amount of contrast in the upper intestine was increased in series A in the glucagon group, but not in series B. It is concluded that glucagon improves visualisation of the biliary tract, especially the gallbladder at infusion cholangiography."}
{"id": "PMID:466953", "title": "Combined fine needle percutaneous transhepatic cholangiography and hypotonic duodenography in obstructive jaundice.", "content": "The information obtained at percutaneous transhepatic cholangiography in patients with obstructive jaundice is not always conclusive about the nature of the obstructing lesion. Hypotonic duodenography performed immediately after transhepatic cholangiography may assist in such cases by demonstrating the presence or absence of duodenal involvement. This ensures that the appropriate surgical procedure can be planned before operation. Our experience using the combined techniques in nine patients is reported.", "contents": "Combined fine needle percutaneous transhepatic cholangiography and hypotonic duodenography in obstructive jaundice. The information obtained at percutaneous transhepatic cholangiography in patients with obstructive jaundice is not always conclusive about the nature of the obstructing lesion. Hypotonic duodenography performed immediately after transhepatic cholangiography may assist in such cases by demonstrating the presence or absence of duodenal involvement. This ensures that the appropriate surgical procedure can be planned before operation. Our experience using the combined techniques in nine patients is reported."}
{"id": "PMID:466954", "title": "Soft-tissue measurements at the index finger: age and sex characteristics.", "content": "Measurements of the soft-tissue width and phalanx width of the index finger were made in 402 normal individuals (270 women and 132 men, age range 20--89 years) in order to establish age-sex characteristics. Men have a significantly larger total finger and phalanx width than women in all age groups. In both sexes a statistically significant increase with age in phalanx width and total finger width is found. The soft-tissue index and soft-tissue thickness is not influenced by age.", "contents": "Soft-tissue measurements at the index finger: age and sex characteristics. Measurements of the soft-tissue width and phalanx width of the index finger were made in 402 normal individuals (270 women and 132 men, age range 20--89 years) in order to establish age-sex characteristics. Men have a significantly larger total finger and phalanx width than women in all age groups. In both sexes a statistically significant increase with age in phalanx width and total finger width is found. The soft-tissue index and soft-tissue thickness is not influenced by age."}
{"id": "PMID:466955", "title": "Radiogrammetry of the soft tissue at the index finger in acromegaly.", "content": "Measurements of the soft-tissue width and phalanx width at the index finger were made in 30 acromegalic patients and compared with values obtained in a large control population. The width of the phalanx, total finger width and soft-tissue thickness are significantly larger than expected and the soft-tissue index values are significantly lower than expected. An important overlap between normal and acromegalic values is found for the soft-tissue index and width of the proximal phalanx. The measurement which best discriminates acromegalics from normal is the total finger width. The soft-tissue thickness parameter is also very sensitive for the detection of acromegalic changes and has the advantage of not being sex and age dependent.", "contents": "Radiogrammetry of the soft tissue at the index finger in acromegaly. Measurements of the soft-tissue width and phalanx width at the index finger were made in 30 acromegalic patients and compared with values obtained in a large control population. The width of the phalanx, total finger width and soft-tissue thickness are significantly larger than expected and the soft-tissue index values are significantly lower than expected. An important overlap between normal and acromegalic values is found for the soft-tissue index and width of the proximal phalanx. The measurement which best discriminates acromegalics from normal is the total finger width. The soft-tissue thickness parameter is also very sensitive for the detection of acromegalic changes and has the advantage of not being sex and age dependent."}
{"id": "PMID:466956", "title": "The anterior costal margin mass in patients with thalassaemia major.", "content": "In thalassaemia major, extramedullary haematopoiesis in the form of tumour-like masses, usually occurs in the posterior mediastinum. We present three cases in which such masses were found at the anterior part of the ribs. The radiological signs of this latter location, which is the second most common after the mediastinum, are described.", "contents": "The anterior costal margin mass in patients with thalassaemia major. In thalassaemia major, extramedullary haematopoiesis in the form of tumour-like masses, usually occurs in the posterior mediastinum. We present three cases in which such masses were found at the anterior part of the ribs. The radiological signs of this latter location, which is the second most common after the mediastinum, are described."}
{"id": "PMID:466957", "title": "Inferior caval venography in the assessment of renal carcinoma.", "content": "Inferior caval venography by a non-catheter technique was carried out in 30 cases of renal cell carcinoma. Occlusion of the renal vein was shown in 15 of these and occlusion of the inferior vena cava in a further three. Deformity of the vena cava by adjacent growth was found in nine instances. Renal arteriography was performed in 27 of these patients and the demonstration of the renal vein by this method has been compared with that of caval venography. The incidence of collateral veins on the arteriogram and the finding of impaired renal function as an index of renal vein occlusion has been discussed.", "contents": "Inferior caval venography in the assessment of renal carcinoma. Inferior caval venography by a non-catheter technique was carried out in 30 cases of renal cell carcinoma. Occlusion of the renal vein was shown in 15 of these and occlusion of the inferior vena cava in a further three. Deformity of the vena cava by adjacent growth was found in nine instances. Renal arteriography was performed in 27 of these patients and the demonstration of the renal vein by this method has been compared with that of caval venography. The incidence of collateral veins on the arteriogram and the finding of impaired renal function as an index of renal vein occlusion has been discussed."}
{"id": "PMID:466958", "title": "The urinary tract in haemophilia.", "content": "Scant attention has been paid to changes in the urinary tract in haemophilia, although haematuria, renal colic, the passage of clots and clot retention have long been known. Twenty-eight haemophiliac subjects were investigated with a total of 59 radiological examinations of the urinary tract including 41 intravenous urograms (IVU). In 10 patients (36%) abnormalities were demonstrated in the urinary tract, six patients showed changes of hydronephrosis, one a non-functioning kidney and in two patients blood clots were shown in the collecting system. These abnormalities were more common in clinically severe haematuria, while normal urographic appearances were common in clinically mild cases. The cause of hydroephrosis was extrinsic from compression, either renal or ureteric, or instrinsic from clot retention. In some cases the cause was not known. It is suggested that in these the hydronephrosis may result from organisation of the haematoma with peri-ureteric fibrosis.", "contents": "The urinary tract in haemophilia. Scant attention has been paid to changes in the urinary tract in haemophilia, although haematuria, renal colic, the passage of clots and clot retention have long been known. Twenty-eight haemophiliac subjects were investigated with a total of 59 radiological examinations of the urinary tract including 41 intravenous urograms (IVU). In 10 patients (36%) abnormalities were demonstrated in the urinary tract, six patients showed changes of hydronephrosis, one a non-functioning kidney and in two patients blood clots were shown in the collecting system. These abnormalities were more common in clinically severe haematuria, while normal urographic appearances were common in clinically mild cases. The cause of hydroephrosis was extrinsic from compression, either renal or ureteric, or instrinsic from clot retention. In some cases the cause was not known. It is suggested that in these the hydronephrosis may result from organisation of the haematoma with peri-ureteric fibrosis."}
{"id": "PMID:466959", "title": "Grey scale ultrasonography and arthrography in evaluation of popliteal cysts.", "content": "Ultrasonography will reliably detect popliteal cysts of clinically significant size and improvements in ultrasound imaging have enhanced the value of ultrasound in relation to arthrography in the assessment of cyst rupture. Forty-eight knees in 25 patients suspected of having a popliteal cyst were examined by ultrasonography followed immediately by arthrography. Popliteal cysts were demonstrated in 40% (19/48) by ultrasound and in 46% (22/48) by arthrogram. Arthrography detected small cysts not seen with ultrasound, but altrasonic scanning showed cysts which did not fill on arthrography. A ruptured cyst or deep venous thrombosis was suspected in 10 patients. Rupture was confirmed in two patients by arthrography, in both of whom soft tissue changes and attenuation of the distal margin of the cysts were shown by ultrasound.", "contents": "Grey scale ultrasonography and arthrography in evaluation of popliteal cysts. Ultrasonography will reliably detect popliteal cysts of clinically significant size and improvements in ultrasound imaging have enhanced the value of ultrasound in relation to arthrography in the assessment of cyst rupture. Forty-eight knees in 25 patients suspected of having a popliteal cyst were examined by ultrasonography followed immediately by arthrography. Popliteal cysts were demonstrated in 40% (19/48) by ultrasound and in 46% (22/48) by arthrogram. Arthrography detected small cysts not seen with ultrasound, but altrasonic scanning showed cysts which did not fill on arthrography. A ruptured cyst or deep venous thrombosis was suspected in 10 patients. Rupture was confirmed in two patients by arthrography, in both of whom soft tissue changes and attenuation of the distal margin of the cysts were shown by ultrasound."}
{"id": "PMID:466960", "title": "The radiology of external carotid-internal carotid anastomosis.", "content": "Surgical anastomosis between branches of the external and internal carotid arteries may be beneficial in selected cases of transient ischaemic attacks and reversible ischaemic neurological deficit. It is the responsibility of the radiologist, at the time of initial angiographic evaluation, to recognise patients who might benefit from this procedure. Postoperatively, angiography is valuable in assessing the surgical results. This report describes the investigations necessary for the adequate evaluation of these patients in both the pre- and post-operative state.", "contents": "The radiology of external carotid-internal carotid anastomosis. Surgical anastomosis between branches of the external and internal carotid arteries may be beneficial in selected cases of transient ischaemic attacks and reversible ischaemic neurological deficit. It is the responsibility of the radiologist, at the time of initial angiographic evaluation, to recognise patients who might benefit from this procedure. Postoperatively, angiography is valuable in assessing the surgical results. This report describes the investigations necessary for the adequate evaluation of these patients in both the pre- and post-operative state."}
{"id": "PMID:466961", "title": "Computerised tomography of intracranial tuberculomata.", "content": "The CT appearances of 14 tuberculomata are described. In 10 of these there is a similarity of appearance which may permit a definitive or high probability diagnosis. The features are: a mass lesion approximately isodense with brain substance surrounded by an intense unbroken ring of contrast enhancement. Calcification may be present within the ring giving a 'target sign'. Two of the other cases were 'tuberculomata en plaque' which had features similar to each other.", "contents": "Computerised tomography of intracranial tuberculomata. The CT appearances of 14 tuberculomata are described. In 10 of these there is a similarity of appearance which may permit a definitive or high probability diagnosis. The features are: a mass lesion approximately isodense with brain substance surrounded by an intense unbroken ring of contrast enhancement. Calcification may be present within the ring giving a 'target sign'. Two of the other cases were 'tuberculomata en plaque' which had features similar to each other."}
{"id": "PMID:466962", "title": "The acute radiation syndrome. A memorial to William Michael Court-Brown.", "content": "The pattern of symptoms in 189 patients irradiated with a large single dose to one half of the body are described. Radiation sickness developed in 83 and 39% of upper half-body irradiation and lower half-body irradiation treatments respectively and the incidence is not reduced by sedatives or anti-emetics. Following treatment there is a latent period up to 90 min before a phase of acute upset and nausea and vomiting starts. This phase persists for about 6h with an average of six bouts of vomiting and is then followed by recovery.", "contents": "The acute radiation syndrome. A memorial to William Michael Court-Brown. The pattern of symptoms in 189 patients irradiated with a large single dose to one half of the body are described. Radiation sickness developed in 83 and 39% of upper half-body irradiation and lower half-body irradiation treatments respectively and the incidence is not reduced by sedatives or anti-emetics. Following treatment there is a latent period up to 90 min before a phase of acute upset and nausea and vomiting starts. This phase persists for about 6h with an average of six bouts of vomiting and is then followed by recovery."}
{"id": "PMID:466963", "title": "A review of thirty-three cases of ovarian dysgerminoma emphasising the role of radiotherapy.", "content": "The records of 33 cases of ovarian dysgerminoma seen in Manchester between 1943 and 1977 are reviewed, including details of presenting features, age distribution, treatment and survival. The five-year survival rate is 85%. All the Stage I cases and all patients under the age of 20 years at presentation have survived. The cases of the five patients who died are analysed in detail. From this series and review of other series it is concluded that young patients with unilateral Stage I desease who have had a total resection should be carefully followed and radiotherapy reserved for recurrent disease. This does not appear to affect their high chance of cure and enable patients who remain disease-free to retain one functioning ovary. All other patients should have surgery followed by radiotherapy to the whole abdomen. Analysis of the five deaths suggested that, even in Stage III disease a surgical attempt should be made to remove the bulk of the tumour and that the abdominal radiotherapy dose should not be less than 3000 rad in 20 treatments in 28 days.", "contents": "A review of thirty-three cases of ovarian dysgerminoma emphasising the role of radiotherapy. The records of 33 cases of ovarian dysgerminoma seen in Manchester between 1943 and 1977 are reviewed, including details of presenting features, age distribution, treatment and survival. The five-year survival rate is 85%. All the Stage I cases and all patients under the age of 20 years at presentation have survived. The cases of the five patients who died are analysed in detail. From this series and review of other series it is concluded that young patients with unilateral Stage I desease who have had a total resection should be carefully followed and radiotherapy reserved for recurrent disease. This does not appear to affect their high chance of cure and enable patients who remain disease-free to retain one functioning ovary. All other patients should have surgery followed by radiotherapy to the whole abdomen. Analysis of the five deaths suggested that, even in Stage III disease a surgical attempt should be made to remove the bulk of the tumour and that the abdominal radiotherapy dose should not be less than 3000 rad in 20 treatments in 28 days."}
{"id": "PMID:466964", "title": "The Leeds results for radiotherapy in HBO for carcinoma of the head and neck.", "content": "Follow-up data at five years are reported for 24 patients with squamous cell carcinoma of the head and neck, included in a randomised prospective MRC study of radiotherapy in hyperbaric oxygen (10 fractions) or air (15 or 20 fractions). Although few, the data show a significant gain in local control (P less than 0.001) and survival (P less than 0.05) with the use of hyperbaric oxygen but no increased tissue reactions or morbidity.", "contents": "The Leeds results for radiotherapy in HBO for carcinoma of the head and neck. Follow-up data at five years are reported for 24 patients with squamous cell carcinoma of the head and neck, included in a randomised prospective MRC study of radiotherapy in hyperbaric oxygen (10 fractions) or air (15 or 20 fractions). Although few, the data show a significant gain in local control (P less than 0.001) and survival (P less than 0.05) with the use of hyperbaric oxygen but no increased tissue reactions or morbidity."}
{"id": "PMID:466965", "title": "Some radiological abnormalities of the cervical carotid artery and their clinical significance.", "content": "Two hundred and fifty-four patients who underwent carotid angiography for common neurological and neurosurgical disorders were reviewed. The incidence and angiographic features of loops, coils, kinks and atheroma are analysed. Loops are common (34%), coils and kinks are not uncommon (7 and 6.6% respectively). The aetiology of these conditions and their clinical importance are discussed. Loops and coils have a developmental basis and do not by themselves cause cerebrovascular ischaemic attacks or influence the patchy development or atheroma. Kinks are acquired and are frequently seen in association with loops but their cause has not been established.", "contents": "Some radiological abnormalities of the cervical carotid artery and their clinical significance. Two hundred and fifty-four patients who underwent carotid angiography for common neurological and neurosurgical disorders were reviewed. The incidence and angiographic features of loops, coils, kinks and atheroma are analysed. Loops are common (34%), coils and kinks are not uncommon (7 and 6.6% respectively). The aetiology of these conditions and their clinical importance are discussed. Loops and coils have a developmental basis and do not by themselves cause cerebrovascular ischaemic attacks or influence the patchy development or atheroma. Kinks are acquired and are frequently seen in association with loops but their cause has not been established."}
{"id": "PMID:466977", "title": "Verapamil acute self-poisoning.", "content": "A previously healthy girl, without heart disease, who ingested 2400 mg of verapamil developed hypotension, trifasicular block pattern, mental confusion, mild metabolic acidosis, and hyperglycemia. She recovered with symptomatic and supportive therapy. A discussion is presented about the action mechanism of the drug.", "contents": "Verapamil acute self-poisoning. A previously healthy girl, without heart disease, who ingested 2400 mg of verapamil developed hypotension, trifasicular block pattern, mental confusion, mild metabolic acidosis, and hyperglycemia. She recovered with symptomatic and supportive therapy. A discussion is presented about the action mechanism of the drug."}
{"id": "PMID:466978", "title": "Severe poisoning in a child caused by a small dose of clonidine.", "content": "A girl aged 1 year 9 months ingested a single tablet of 0.3 mg of clonidine. She became soon drowsy and unconscious with bradycardia and severe hypotension. Bradycardia was antagonized promptly by atropine, and blood pressure was corrected by giving intravenous fluids. The level of consciousness fluctuated for 6 to 8 hr from alertness to unconsciousness. Serum concentrations of clonidine were only about two times higher than the mean therapeutic values in adults in the treatment of hypertension. Thus small doses of clonidine seem to be potentially dangerous for small children.", "contents": "Severe poisoning in a child caused by a small dose of clonidine. A girl aged 1 year 9 months ingested a single tablet of 0.3 mg of clonidine. She became soon drowsy and unconscious with bradycardia and severe hypotension. Bradycardia was antagonized promptly by atropine, and blood pressure was corrected by giving intravenous fluids. The level of consciousness fluctuated for 6 to 8 hr from alertness to unconsciousness. Serum concentrations of clonidine were only about two times higher than the mean therapeutic values in adults in the treatment of hypertension. Thus small doses of clonidine seem to be potentially dangerous for small children."}
{"id": "PMID:466979", "title": "Estimation of colchicine in a poisoned patient by using high performance liquid chromatography.", "content": "A rapid method was developed for the estimation of colchicine in plasma. The method was used to determine colchicine levels in a fatal case of colchicine self-poisoning.", "contents": "Estimation of colchicine in a poisoned patient by using high performance liquid chromatography. A rapid method was developed for the estimation of colchicine in plasma. The method was used to determine colchicine levels in a fatal case of colchicine self-poisoning."}
{"id": "PMID:466981", "title": "Arsenic exposure in multiple environmental media in children near a smelter.", "content": "A nationwide survey of heavy-metal exposure in children living near primary nonferrous metal smelters demonstrated high urine arsenic levels in children living near a copper smelter in Ajo, Arizona. Airborne smelter emissions and drinking water were the apparent sources of exposure. To determine whether increased arsenic absorption had produced adverse health effects, we conducted an evaluation of 132 Ajo children 5 to 18 years old and compared results with those of 47 children from a comparison town with low arsenic exposure. Environmental testing showed that Ajo's municipal water supply contained arsenic in concentrations of 0.09 mg/l (the EPA standard is 0.05 mg/l); arsenic concentrations in dust averaged 342.2 microgram/g. Urine arsenic levels in Ajo children correlated positively with amount of tap-water consumed (r = .32, p less than. 0002) and with distance of residence from the smelter (r = .20, p less than .02). Tap-water drinkers had significantly higher urine arsenic levels than bottled water drinkers (t = 4.21 p less than .001). Mean urine arsenic levels were significantly higher for children in Ajo (4.75 microgram/100 ml) than for children in the comparison town (1.17 microgram/100 ml). Hair arsenic levels correlated poorly with arsenic exposure. Despite the study population's chronic exposure to elevated environmental levels of arsenic, no clinical or hematologic abnormalities attributable to arsenic were found.", "contents": "Arsenic exposure in multiple environmental media in children near a smelter. A nationwide survey of heavy-metal exposure in children living near primary nonferrous metal smelters demonstrated high urine arsenic levels in children living near a copper smelter in Ajo, Arizona. Airborne smelter emissions and drinking water were the apparent sources of exposure. To determine whether increased arsenic absorption had produced adverse health effects, we conducted an evaluation of 132 Ajo children 5 to 18 years old and compared results with those of 47 children from a comparison town with low arsenic exposure. Environmental testing showed that Ajo's municipal water supply contained arsenic in concentrations of 0.09 mg/l (the EPA standard is 0.05 mg/l); arsenic concentrations in dust averaged 342.2 microgram/g. Urine arsenic levels in Ajo children correlated positively with amount of tap-water consumed (r = .32, p less than. 0002) and with distance of residence from the smelter (r = .20, p less than .02). Tap-water drinkers had significantly higher urine arsenic levels than bottled water drinkers (t = 4.21 p less than .001). Mean urine arsenic levels were significantly higher for children in Ajo (4.75 microgram/100 ml) than for children in the comparison town (1.17 microgram/100 ml). Hair arsenic levels correlated poorly with arsenic exposure. Despite the study population's chronic exposure to elevated environmental levels of arsenic, no clinical or hematologic abnormalities attributable to arsenic were found."}
{"id": "PMID:466982", "title": "Effects on the offspring of chronic low exposure carbon monoxide during mice pregnancy.", "content": "This research is primarily concerned with the effects of chronic low doses of carbon monoxide on fetal development. Carbon monoxide was administered daily by inhalation to female Swiss Webster mice from the beginning of gestation until term. Daily weights were recorded and carbon monoxide blood levels determined every 4 days. The number of offspring in each litter was recorded. At weaning, two males and two females from each litter were randomly picked for maze running studies. When the mice were 6 weeks old, they were tested daily, ten trials per day, in the maze until learning had occurred. The number of days required to learn the maze and the number of incorrect trials were recorded. While there was no significant increase in the number of days needed to learn the maze, there was a significant increase in the number of errors made by the experimental group during this time. This indicated that an increased effort was needed to learn the maze.", "contents": "Effects on the offspring of chronic low exposure carbon monoxide during mice pregnancy. This research is primarily concerned with the effects of chronic low doses of carbon monoxide on fetal development. Carbon monoxide was administered daily by inhalation to female Swiss Webster mice from the beginning of gestation until term. Daily weights were recorded and carbon monoxide blood levels determined every 4 days. The number of offspring in each litter was recorded. At weaning, two males and two females from each litter were randomly picked for maze running studies. When the mice were 6 weeks old, they were tested daily, ten trials per day, in the maze until learning had occurred. The number of days required to learn the maze and the number of incorrect trials were recorded. While there was no significant increase in the number of days needed to learn the maze, there was a significant increase in the number of errors made by the experimental group during this time. This indicated that an increased effort was needed to learn the maze."}
{"id": "PMID:466984", "title": "Myocardial infarction and marijuana.", "content": "Myocardial infarction in the virtual absence of risk factors occurred in a 25-year old man shortly after smoking a cigarette containing marijuana. Subsequent coronary arteriography was normal.", "contents": "Myocardial infarction and marijuana. Myocardial infarction in the virtual absence of risk factors occurred in a 25-year old man shortly after smoking a cigarette containing marijuana. Subsequent coronary arteriography was normal."}
{"id": "PMID:466985", "title": "Additional conclusions on diazepam injectable precipitate: GC-MS confirmation.", "content": "The precipitate which forms upon dilution of diazepam injection with aqueous vehicles is shown conclusively to be diazepam and not benzoates. The results are based on GC-MS analysis of the precipitate as compared with a synthetic mixture of benzoic acid-diazepam.", "contents": "Additional conclusions on diazepam injectable precipitate: GC-MS confirmation. The precipitate which forms upon dilution of diazepam injection with aqueous vehicles is shown conclusively to be diazepam and not benzoates. The results are based on GC-MS analysis of the precipitate as compared with a synthetic mixture of benzoic acid-diazepam."}
{"id": "PMID:466989", "title": "Special classroom placements for EMR children: guidelines for community mental health personnel.", "content": "Community mental health personnel have become increasingly involved in public school interventions for mildly retarded children. Previous research, legal findings, and ethical principles regarding the most common intervention, special class placement, are reviewed. Advantages and disadvantages of special placement are presented. Finally, a process model is proposed as offering guidelines to the mental health professional who is involved in establishing treatment programs for EMR children in the public school.", "contents": "Special classroom placements for EMR children: guidelines for community mental health personnel. Community mental health personnel have become increasingly involved in public school interventions for mildly retarded children. Previous research, legal findings, and ethical principles regarding the most common intervention, special class placement, are reviewed. Advantages and disadvantages of special placement are presented. Finally, a process model is proposed as offering guidelines to the mental health professional who is involved in establishing treatment programs for EMR children in the public school."}
{"id": "PMID:466991", "title": "Social workers in primary prevention: action and ideology in mental health.", "content": "Primary prevention and a public health model have been among the distinguishing innovations of the community mental health movement. Social work practice, however, has historically been involved in community intervention and environmental manipulation to offset social and psychological jeopardy. Given a long tradition of commitment to activity and techniques which are now hailed as mental health's \"third revolution,\" this study explores the role of social work in primary prevention. Data are from a survey of three community mental health centers in which professional staff completed the Gottesfeld Critical Issues of Community Mental Health questionnaire, a time-distribution form, and a prevention questionnaire. In addition, all staff working in primary prevention were interviewed in depth. The total sample of this study of mental health professionals was 84. This study points to some interesting contradictions found between social work action and ideology in primary prevention. Also, social workers are compared with other professionals in order to isolate some primary prevention tasks and attitudes that appear unique to each.", "contents": "Social workers in primary prevention: action and ideology in mental health. Primary prevention and a public health model have been among the distinguishing innovations of the community mental health movement. Social work practice, however, has historically been involved in community intervention and environmental manipulation to offset social and psychological jeopardy. Given a long tradition of commitment to activity and techniques which are now hailed as mental health's \"third revolution,\" this study explores the role of social work in primary prevention. Data are from a survey of three community mental health centers in which professional staff completed the Gottesfeld Critical Issues of Community Mental Health questionnaire, a time-distribution form, and a prevention questionnaire. In addition, all staff working in primary prevention were interviewed in depth. The total sample of this study of mental health professionals was 84. This study points to some interesting contradictions found between social work action and ideology in primary prevention. Also, social workers are compared with other professionals in order to isolate some primary prevention tasks and attitudes that appear unique to each."}
{"id": "PMID:466992", "title": "Family psychotherapy with the homosexual family: a community psychiatry approach to homosexuality.", "content": "The author points out that homosexual families are not being treated by family psychotherapy despite an indicated need. This situation is inconsistent with good community psychiatry and behooves the mental health workers to become aware of and to work through his unrecognized negative feelings which are interfering with provision of services. Among the main resistances are the lack of familiarity and comfort with the family psychotherapy technique, inability to resist a greater gratification that comes from focusing on sex rather than on a less titillating relationship issue, and judgmental and condemning attitudes toward homosexuality that come from intrapsychic and societal sources.", "contents": "Family psychotherapy with the homosexual family: a community psychiatry approach to homosexuality. The author points out that homosexual families are not being treated by family psychotherapy despite an indicated need. This situation is inconsistent with good community psychiatry and behooves the mental health workers to become aware of and to work through his unrecognized negative feelings which are interfering with provision of services. Among the main resistances are the lack of familiarity and comfort with the family psychotherapy technique, inability to resist a greater gratification that comes from focusing on sex rather than on a less titillating relationship issue, and judgmental and condemning attitudes toward homosexuality that come from intrapsychic and societal sources."}
{"id": "PMID:466993", "title": "Relationship of sociodemographic and drinking variables to differentiated subgroups of alcoholics.", "content": "The results suggest that the sociodemographic variables of age, living arrangements, number of dependents, the drinking variable, and the client's perception of the type of drinker he is are the most strongly related to the derived typology. The exact relationship of these variables, as well as the variables that were moderately related to the typology, is not apparent. There is a pattern, however, that appears to run through the categories of the classification. The evidence suggests that for alcoholics to be in their prime earning years, living with their spouses, perceiving themselves as alcoholic, possessing marketable skills, and a recent history of abstinence seems to increase the likelihood of psychological need satisfaction. The antithesis--to be alcoholic, less than 30 or older than 50 years old, living by oneself or with parents, perceiving oneself as a social or problem drinker, and possessing a recent history of drinking--seems to decrease the likelihood of psychological need satisfaction. There seems to be a relationship between drinking behavior and psychological need satisfaction. As a group, the members of Clusters Two, Three, and Four are unable to abstain for longer than a week; Clusters Two and Three both possess physiological problems of adjustment. The members of Clusters One and Five tend to be able to abstain for longer periods. Cluster One is devoid of physiological difficulties, whereas Five has borderline problems. The physiological subscale of the Human Service Scale assesses the absence of symptoms associated with poor health (Realges & Butler, 1976). It appears that aberrant drinking does not necessarily result in self-perceived physiological-health complaints. These results are at variance with the belief that ethanol ingestion by an alcoholic and the resulting pathological complications are the predominant difficulties encountered by these persons. Thus it seems to us that a distraught life situation (that is, the lack of social and demographic assets--marriage, education, and so on) contributes to self-perceived severity of psychological need deprivation and not solely aberrant drinking. Therefore, in terms of recovery from alcoholism, these results argue against a unidimensional approach toward the treatment of alcoholism; that is, an approach that focuses solely on the drinking as \"the\" problem. A more strategic approach would include a thorough medical, psychiatric, psychological, social and vocational evaluation of the alcoholic's life situation, which in turn would permit mobilizing the efforts of a multidisciplinary team whose goal would be to maximize the resources and enhance the life situation of the alcoholic. It is hoped that the results of this study serve to increase our understanding of those factors that influence aberrant drinking.", "contents": "Relationship of sociodemographic and drinking variables to differentiated subgroups of alcoholics. The results suggest that the sociodemographic variables of age, living arrangements, number of dependents, the drinking variable, and the client's perception of the type of drinker he is are the most strongly related to the derived typology. The exact relationship of these variables, as well as the variables that were moderately related to the typology, is not apparent. There is a pattern, however, that appears to run through the categories of the classification. The evidence suggests that for alcoholics to be in their prime earning years, living with their spouses, perceiving themselves as alcoholic, possessing marketable skills, and a recent history of abstinence seems to increase the likelihood of psychological need satisfaction. The antithesis--to be alcoholic, less than 30 or older than 50 years old, living by oneself or with parents, perceiving oneself as a social or problem drinker, and possessing a recent history of drinking--seems to decrease the likelihood of psychological need satisfaction. There seems to be a relationship between drinking behavior and psychological need satisfaction. As a group, the members of Clusters Two, Three, and Four are unable to abstain for longer than a week; Clusters Two and Three both possess physiological problems of adjustment. The members of Clusters One and Five tend to be able to abstain for longer periods. Cluster One is devoid of physiological difficulties, whereas Five has borderline problems. The physiological subscale of the Human Service Scale assesses the absence of symptoms associated with poor health (Realges & Butler, 1976). It appears that aberrant drinking does not necessarily result in self-perceived physiological-health complaints. These results are at variance with the belief that ethanol ingestion by an alcoholic and the resulting pathological complications are the predominant difficulties encountered by these persons. Thus it seems to us that a distraught life situation (that is, the lack of social and demographic assets--marriage, education, and so on) contributes to self-perceived severity of psychological need deprivation and not solely aberrant drinking. Therefore, in terms of recovery from alcoholism, these results argue against a unidimensional approach toward the treatment of alcoholism; that is, an approach that focuses solely on the drinking as \"the\" problem. A more strategic approach would include a thorough medical, psychiatric, psychological, social and vocational evaluation of the alcoholic's life situation, which in turn would permit mobilizing the efforts of a multidisciplinary team whose goal would be to maximize the resources and enhance the life situation of the alcoholic. It is hoped that the results of this study serve to increase our understanding of those factors that influence aberrant drinking."}
{"id": "PMID:466994", "title": "Consultation and education in rural community mental health centers.", "content": "This paper reports on the status of consultation and education in the rural community mental health center: First, it describes the rural programs on several organizational dimensions as compared to nonrural programs. Second, it identifies several distinctive characteristics of rural programs. Third, it presents a case illustration of a successful consultation-education service. Finally, it discusses strategies for strengthening consultation and education in the rural setting. The paper is based on the findings of a study of prevention programs in community mental health centers in Region III (Pensylvania, Delaware, Maryland, Washington, D.C., Virginia, and West Virginia).", "contents": "Consultation and education in rural community mental health centers. This paper reports on the status of consultation and education in the rural community mental health center: First, it describes the rural programs on several organizational dimensions as compared to nonrural programs. Second, it identifies several distinctive characteristics of rural programs. Third, it presents a case illustration of a successful consultation-education service. Finally, it discusses strategies for strengthening consultation and education in the rural setting. The paper is based on the findings of a study of prevention programs in community mental health centers in Region III (Pensylvania, Delaware, Maryland, Washington, D.C., Virginia, and West Virginia)."}
{"id": "PMID:466995", "title": "Awareness, use, and consequences of evaluation data in a community mental health center.", "content": "A major aspect of the effort of the Mental Health Systems Evaluation Project of the Northwest Denver Community Health Center's has involved studying the utilization of program evaluation data by community mental health center managers and clinicians. The intent of this effort was twofold: to assess the impact of program evaluation feedback in the community mental health center per se; and to investigate factors that influenced this impact. The impact of such input was assessed initially at the level of \"subjective utility.\" Later attempts involved more extended or interactive forms of input and impact assessment. Basic findings to date suggest that program evaluation data are most likely to have impact when directed at the managers most closely involved with the service at issue, and when presented, preferably simply and personally, in ways that insure an understanding of the data and its implications by those receiving it.", "contents": "Awareness, use, and consequences of evaluation data in a community mental health center. A major aspect of the effort of the Mental Health Systems Evaluation Project of the Northwest Denver Community Health Center's has involved studying the utilization of program evaluation data by community mental health center managers and clinicians. The intent of this effort was twofold: to assess the impact of program evaluation feedback in the community mental health center per se; and to investigate factors that influenced this impact. The impact of such input was assessed initially at the level of \"subjective utility.\" Later attempts involved more extended or interactive forms of input and impact assessment. Basic findings to date suggest that program evaluation data are most likely to have impact when directed at the managers most closely involved with the service at issue, and when presented, preferably simply and personally, in ways that insure an understanding of the data and its implications by those receiving it."}
{"id": "PMID:467005", "title": "The efficient numerical solution of biological simulation problems.", "content": "A subroutine called DESOL for the numerical solution of ordinary differential equations of the type arising in biological simulation problems is described. DESOL is about as efficient as current high quality integrators, but because of its compactness it can be easily used on small computers. The subroutine has excellent stability properties and functions with very little required input from the user. In addition, it has features which aid in debugging associated programs. Several test and example problems are given, as is the derivation of the major formulae used in the package.", "contents": "The efficient numerical solution of biological simulation problems. A subroutine called DESOL for the numerical solution of ordinary differential equations of the type arising in biological simulation problems is described. DESOL is about as efficient as current high quality integrators, but because of its compactness it can be easily used on small computers. The subroutine has excellent stability properties and functions with very little required input from the user. In addition, it has features which aid in debugging associated programs. Several test and example problems are given, as is the derivation of the major formulae used in the package."}
{"id": "PMID:467006", "title": "Computer programs for nucleic acids studies. Atomic coordinates of helices and their graphic display.", "content": "For the purpose of calculation of NMR and other physiocochemical properties of nucleic acids, a computer program in FORTRAN language has been written. This program provides the printout of the Cartesian and cylindrical coordinates of all atoms of a double-stranded helix of nucleic acid in either A, A' or B conformation with any specified base sequence up to 50 nucleotides or longer. In addition, the interatomic distances between any two atoms or distances (with both perpendicular and parallel components) from the centers of the base rings to any atom in the helix can be calculated. This information has been used for the calculation of the ring current effects of the 1H chemical shift of two short helices. Satisfactory agreement has been found in the comparison between the present data and that obtained from model construction and from the table prepared by Arter and Schmidt. The structure of the helix can also be illustrated in graphic form on a Tektronix 4006 CRT terminal. The presentation can be manipulated, such as selection, enlargement, translation and rotation.", "contents": "Computer programs for nucleic acids studies. Atomic coordinates of helices and their graphic display. For the purpose of calculation of NMR and other physiocochemical properties of nucleic acids, a computer program in FORTRAN language has been written. This program provides the printout of the Cartesian and cylindrical coordinates of all atoms of a double-stranded helix of nucleic acid in either A, A' or B conformation with any specified base sequence up to 50 nucleotides or longer. In addition, the interatomic distances between any two atoms or distances (with both perpendicular and parallel components) from the centers of the base rings to any atom in the helix can be calculated. This information has been used for the calculation of the ring current effects of the 1H chemical shift of two short helices. Satisfactory agreement has been found in the comparison between the present data and that obtained from model construction and from the table prepared by Arter and Schmidt. The structure of the helix can also be illustrated in graphic form on a Tektronix 4006 CRT terminal. The presentation can be manipulated, such as selection, enlargement, translation and rotation."}
{"id": "PMID:467007", "title": "Computer simulated modeling of biomolecular systems.", "content": "This paper describes the algorithm of a program used to simulate three dimensional models of molecules. In addition to open ended molecules the program also enables simulation of structures with constraints in the form of cyclic regions or fixed location of particular atoms. Several molecules can be handled in a single run and each molecule can have any number of contraints. Further, any number of conformations can be obtained for each constrained region. The program can be used for research in several areas of molecular biology, e.g., structure determination, conformational analysis and topographic comparisons.", "contents": "Computer simulated modeling of biomolecular systems. This paper describes the algorithm of a program used to simulate three dimensional models of molecules. In addition to open ended molecules the program also enables simulation of structures with constraints in the form of cyclic regions or fixed location of particular atoms. Several molecules can be handled in a single run and each molecule can have any number of contraints. Further, any number of conformations can be obtained for each constrained region. The program can be used for research in several areas of molecular biology, e.g., structure determination, conformational analysis and topographic comparisons."}
{"id": "PMID:467008", "title": "A computer program for topographic analysis of biomolecular systems.", "content": "This program essentially generates a picture of a space filling molecular model. By precise simulation of the intersections between atoms, leaving out the 'hidden line', a good perspective is produced. Additional three dimensional information is provided by simulating reflection spots on the surface. This program has been used to visualise three dimensional surface features of biologically important molecules (e.g., valinomycin). With slight modification the program can be used to visualise enzyme active sites and receptor surfaces. It can also be used to create animated motion pictures of molecular dynamics.", "contents": "A computer program for topographic analysis of biomolecular systems. This program essentially generates a picture of a space filling molecular model. By precise simulation of the intersections between atoms, leaving out the 'hidden line', a good perspective is produced. Additional three dimensional information is provided by simulating reflection spots on the surface. This program has been used to visualise three dimensional surface features of biologically important molecules (e.g., valinomycin). With slight modification the program can be used to visualise enzyme active sites and receptor surfaces. It can also be used to create animated motion pictures of molecular dynamics."}
{"id": "PMID:467009", "title": "Discriminant analysis: methods and program.", "content": "Because of the disparity in the results obtained using different methods of discriminant analysis, we have written and used a unique program to test them. These methods can be divided into two groups corresponding to a classical probabilistic approach (i.e., Bayesian methods) or a topological approach (i.e., Sebestyen method).", "contents": "Discriminant analysis: methods and program. Because of the disparity in the results obtained using different methods of discriminant analysis, we have written and used a unique program to test them. These methods can be divided into two groups corresponding to a classical probabilistic approach (i.e., Bayesian methods) or a topological approach (i.e., Sebestyen method)."}
{"id": "PMID:467010", "title": "A general program for estimation of haplotype frequencies from population diploid data.", "content": "The program which is written in FORTRAN estimates haplotype frequencies in two-locus and three-locus genetic systems from population diploid data. It is based on the gene counting method which leads to maximum likelihood estimates, and can be used whenever the possible antigens (one or more) on each chromosome can be specified for each person and for each locus, i.e., ABO-like systems and inclusions are permitted. The number of alleles per locus may be rather large, and both grouped and ungrouped data can be used. Log likelihoods are calculated on the basis of various assumptions, so that likelihood ratio tests can be carried out.", "contents": "A general program for estimation of haplotype frequencies from population diploid data. The program which is written in FORTRAN estimates haplotype frequencies in two-locus and three-locus genetic systems from population diploid data. It is based on the gene counting method which leads to maximum likelihood estimates, and can be used whenever the possible antigens (one or more) on each chromosome can be specified for each person and for each locus, i.e., ABO-like systems and inclusions are permitted. The number of alleles per locus may be rather large, and both grouped and ungrouped data can be used. Log likelihoods are calculated on the basis of various assumptions, so that likelihood ratio tests can be carried out."}
{"id": "PMID:467011", "title": "Density estimation applications for outlier detection.", "content": "Nonparametric estimates of joint, conditional and marginal probability densities can be used to estimate the relative probability of a data point's recurrence. Outlier, unusual or abnormal values of a random variate tend to be those which are unlikely to recur. As part of an interactive graphical system, a procedure has been implemented which enables a biomedical researcher to view both the estimated probability and the numerical value of a data point's coordinates. This display circumvents the problem of interpreting a normal range in two or more dimensions and can thus be more easily generalized than most alternative outlier detection procedures.", "contents": "Density estimation applications for outlier detection. Nonparametric estimates of joint, conditional and marginal probability densities can be used to estimate the relative probability of a data point's recurrence. Outlier, unusual or abnormal values of a random variate tend to be those which are unlikely to recur. As part of an interactive graphical system, a procedure has been implemented which enables a biomedical researcher to view both the estimated probability and the numerical value of a data point's coordinates. This display circumvents the problem of interpreting a normal range in two or more dimensions and can thus be more easily generalized than most alternative outlier detection procedures."}
{"id": "PMID:467012", "title": "LOGIT: a program for dose-response analysis.", "content": "We describe a FORTRAN computer program for fitting the logistic distribution function: (formula: see text) Where x represents dose or time, to dose-response data. The program determines both weighted least squares and maximum likelihood estimates for the parameters alpha and beta. It also calculates the standard errors of alpha and beta under both estimation methods, as well as the median lethal dose (LD50) and its standard error. Dose--response curves found by both fitting methods can be plotted as well as the 95% confidence bands for these lines.", "contents": "LOGIT: a program for dose-response analysis. We describe a FORTRAN computer program for fitting the logistic distribution function: (formula: see text) Where x represents dose or time, to dose-response data. The program determines both weighted least squares and maximum likelihood estimates for the parameters alpha and beta. It also calculates the standard errors of alpha and beta under both estimation methods, as well as the median lethal dose (LD50) and its standard error. Dose--response curves found by both fitting methods can be plotted as well as the 95% confidence bands for these lines."}
{"id": "PMID:467013", "title": "TFLUX: a general purpose program for the interpretation of radioactive tracer experiments.", "content": "TFLUX is a computer program which simulates radioactive tracer experiments using intact cells under steady-state conditions. It is designed for the naive computer user to use, providing English-like input. An example is described in which compartmentation and flux through the citric acid cycle in Dictyostelium discoideum are simulated.", "contents": "TFLUX: a general purpose program for the interpretation of radioactive tracer experiments. TFLUX is a computer program which simulates radioactive tracer experiments using intact cells under steady-state conditions. It is designed for the naive computer user to use, providing English-like input. An example is described in which compartmentation and flux through the citric acid cycle in Dictyostelium discoideum are simulated."}
{"id": "PMID:467014", "title": "An interactive toxicological data handling system for a PDP-12 computer.", "content": "This paper describes a program developed for the cataloguing, storage and retrieval of toxicological data. The user can, through dialogue with the program, enter and update data, and request reports with statistical analyses. The system is written in the FOCAL-12 language for a PDP-12 laboratory computer, and is suitable for a larger number of moderately-sized experiments.", "contents": "An interactive toxicological data handling system for a PDP-12 computer. This paper describes a program developed for the cataloguing, storage and retrieval of toxicological data. The user can, through dialogue with the program, enter and update data, and request reports with statistical analyses. The system is written in the FOCAL-12 language for a PDP-12 laboratory computer, and is suitable for a larger number of moderately-sized experiments."}
{"id": "PMID:467015", "title": "A computer system for neurosurgical patient monitoring.", "content": "The variables monitored in intensive care units are generally late indicators of neurologic deterioration. A system based on a LINC-8 computer was therefore developed for on-line monitoring of evoked potentials, electroencephalography (EEG), and transcranial and transthoracic impedances as well as conventional parameters. Somatosensory evoked potentials are recorded at either 30 min or 1 h intervals. One minute epochs of EEG are analyzed every 10 min using a peak-detection algorithm. Impedances and conventional parameters are also monitored at 10 min intervals. In a study of 50 patients, the technical feasibility of this type of monitoring with a small laboratory computer has been demonstrated. In some instances, evoked potentials and EEG show changes prior to detectable neurologic changes. The study suggests that this type of monitoring can provide a valuable adjunct for evaluation of physiologic function in neurosurgical intensive care.", "contents": "A computer system for neurosurgical patient monitoring. The variables monitored in intensive care units are generally late indicators of neurologic deterioration. A system based on a LINC-8 computer was therefore developed for on-line monitoring of evoked potentials, electroencephalography (EEG), and transcranial and transthoracic impedances as well as conventional parameters. Somatosensory evoked potentials are recorded at either 30 min or 1 h intervals. One minute epochs of EEG are analyzed every 10 min using a peak-detection algorithm. Impedances and conventional parameters are also monitored at 10 min intervals. In a study of 50 patients, the technical feasibility of this type of monitoring with a small laboratory computer has been demonstrated. In some instances, evoked potentials and EEG show changes prior to detectable neurologic changes. The study suggests that this type of monitoring can provide a valuable adjunct for evaluation of physiologic function in neurosurgical intensive care."}
{"id": "PMID:467016", "title": "Localization in interstitial dosimetry utilizing the CT scanner.", "content": "A simple method utilizing CT scanners which were readily available is described in which alignment of the isodose curves can be made directly on the printout of the CT scan. This has obvious practical and theoretical advantages. In particular, the dose to vital structures, such as spinal cord and other critical viscera, can be well described.", "contents": "Localization in interstitial dosimetry utilizing the CT scanner. A simple method utilizing CT scanners which were readily available is described in which alignment of the isodose curves can be made directly on the printout of the CT scan. This has obvious practical and theoretical advantages. In particular, the dose to vital structures, such as spinal cord and other critical viscera, can be well described."}
{"id": "PMID:467017", "title": "Pre-enhancement ring density in resolving intracerebral hematomas.", "content": "Pathologic material is presented demonstrating a pre-enhancement ring density in later stages of hematoma resolution on CT. This pre-enhancement ring density is due to aggregates of hemosiderinladen macrophages.", "contents": "Pre-enhancement ring density in resolving intracerebral hematomas. Pathologic material is presented demonstrating a pre-enhancement ring density in later stages of hematoma resolution on CT. This pre-enhancement ring density is due to aggregates of hemosiderinladen macrophages."}
{"id": "PMID:467018", "title": "Computed tomography in the diagnosis of intracranial meningioma.", "content": "The CT findings in 53 meningiomas are reported. Based upon the plain and post-contrast CT pattern and density characteristics, the diagnosis of meningioma was accurately predicted in 28 of 35 convexity, parasagittal, subfrontal, middle fossa and sphenoid wing lesions (80%). In 2 calcified nonenhancing meningioma, the diagnosis was suspected by CT findings. CT was extremely accurate in detecting 16 juxtasellar, intraventricular, tentorial and posterior fossa meningiomas, but the CT pattern was less specific such that differentiation from other neoplastic or vascular lesions was not always possible. There were only 2 false negative cases; these occurred when contrast infusion had not been performed.", "contents": "Computed tomography in the diagnosis of intracranial meningioma. The CT findings in 53 meningiomas are reported. Based upon the plain and post-contrast CT pattern and density characteristics, the diagnosis of meningioma was accurately predicted in 28 of 35 convexity, parasagittal, subfrontal, middle fossa and sphenoid wing lesions (80%). In 2 calcified nonenhancing meningioma, the diagnosis was suspected by CT findings. CT was extremely accurate in detecting 16 juxtasellar, intraventricular, tentorial and posterior fossa meningiomas, but the CT pattern was less specific such that differentiation from other neoplastic or vascular lesions was not always possible. There were only 2 false negative cases; these occurred when contrast infusion had not been performed."}
{"id": "PMID:467019", "title": "Computed tomography in the diagnosis of intracranial vascular malformations.", "content": "Thirty-one patients iwth intracranial vascular malformations were studied by computed tomography (CT), including arteriovenous malformations (AVM) (24), Sturge-Weber syndrome (3), vein of Galen malformation (2) and venous angioma (2). Non-contrast CT showed abnormalities in 27 cases consistent with hemorrhage, calcification, low-density cystic spaces, and post-contrast scan showed abnormal enhancement in 25 of 31 cases. In 14 of 25 cases, the enhancement pattern showed evidence of abnormal vessles to indicate the presence of vascular malformation prior to angiography, whereas in 11 cases the enhancement pattern was not sufficiently characteristic.", "contents": "Computed tomography in the diagnosis of intracranial vascular malformations. Thirty-one patients iwth intracranial vascular malformations were studied by computed tomography (CT), including arteriovenous malformations (AVM) (24), Sturge-Weber syndrome (3), vein of Galen malformation (2) and venous angioma (2). Non-contrast CT showed abnormalities in 27 cases consistent with hemorrhage, calcification, low-density cystic spaces, and post-contrast scan showed abnormal enhancement in 25 of 31 cases. In 14 of 25 cases, the enhancement pattern showed evidence of abnormal vessles to indicate the presence of vascular malformation prior to angiography, whereas in 11 cases the enhancement pattern was not sufficiently characteristic."}
{"id": "PMID:467020", "title": "Computerized tomography of retroperitoneal lymphadenopathy: benign or malignant.", "content": "A distinguishing feature has been noted to help differentiate between malignant retroperitoneal nodes and nodes which contain reactive hyperplasia. This sign is the presence of a cleavage plane between the mass of nodes and the great vessels which are easily visible in benign disease. This distinction is in reference to malignant nodal disease where the contours of the vessels are obscured. The ability to make a diagnosis of benign disease on CAT scan has enabled us to choose the appropriate course of therapy and, in some patients, allowed us to avoid exploratory laparotomy for diagnosis.", "contents": "Computerized tomography of retroperitoneal lymphadenopathy: benign or malignant. A distinguishing feature has been noted to help differentiate between malignant retroperitoneal nodes and nodes which contain reactive hyperplasia. This sign is the presence of a cleavage plane between the mass of nodes and the great vessels which are easily visible in benign disease. This distinction is in reference to malignant nodal disease where the contours of the vessels are obscured. The ability to make a diagnosis of benign disease on CAT scan has enabled us to choose the appropriate course of therapy and, in some patients, allowed us to avoid exploratory laparotomy for diagnosis."}
{"id": "PMID:467021", "title": "Double-exposure technique in computerized tomography of the spine.", "content": "The authors present a technique for recording both soft tissue and bone on the same reproduction of CT images of the spine. Enhancement around the edge of osseous structures is a valuable aspect of this technique.", "contents": "Double-exposure technique in computerized tomography of the spine. The authors present a technique for recording both soft tissue and bone on the same reproduction of CT images of the spine. Enhancement around the edge of osseous structures is a valuable aspect of this technique."}
{"id": "PMID:467022", "title": "Chrome sensitivity in Israel.", "content": "Fifty-two Israeli Jewish patients suffering from chrome sensitivity (C.S.) were investigated with regard to their ethnic origin, age of onset of the dermatitis, occupational history and socio-economic level. Forty-eight patients suffering from various skin conditions and drawn from the outpatient dermatological clinic were used as controls. The socio-economic status of C.S. patients was found to be very low, regardless of ethnic origin. Ninety-four percent of the patients were non-Ashkenazi Jews and a significantly increased proportion of those (24%) were of Kurdish descent. There were no major differences in their occupations. Patients of Kurdish origin manifested the disease at a significantly earlier age than other non-Ashkenazi patients, although the socio-economic conditions of the different ethnic groups of patients were similar.", "contents": "Chrome sensitivity in Israel. Fifty-two Israeli Jewish patients suffering from chrome sensitivity (C.S.) were investigated with regard to their ethnic origin, age of onset of the dermatitis, occupational history and socio-economic level. Forty-eight patients suffering from various skin conditions and drawn from the outpatient dermatological clinic were used as controls. The socio-economic status of C.S. patients was found to be very low, regardless of ethnic origin. Ninety-four percent of the patients were non-Ashkenazi Jews and a significantly increased proportion of those (24%) were of Kurdish descent. There were no major differences in their occupations. Patients of Kurdish origin manifested the disease at a significantly earlier age than other non-Ashkenazi patients, although the socio-economic conditions of the different ethnic groups of patients were similar."}
{"id": "PMID:467023", "title": "Nickel allergy and osteomyelitis in a patient with metal osteosynthesis of a jaw fracture.", "content": "A 27-year-old female, after a fracture of the mandible treated with osteosynthesis and after a difficult extraction, developed osteomyelitis with fistulation and sequestration. Sequestrectomy and extraction of the teeth in the osteomyelitic area did not clear the patient of symptoms. A patch test to nickel turned out to be positive. After removal of the stainless steel cerclage in the mandible, clinical and radiographic changes returned to normal within 2 and 8 weeks, respectively. It is concluded that, whenever acute treatment does not take priority, patch tests comprising nickel, cobalt and chromium should be performed in patients who are candidates for metal osteosynthesis.", "contents": "Nickel allergy and osteomyelitis in a patient with metal osteosynthesis of a jaw fracture. A 27-year-old female, after a fracture of the mandible treated with osteosynthesis and after a difficult extraction, developed osteomyelitis with fistulation and sequestration. Sequestrectomy and extraction of the teeth in the osteomyelitic area did not clear the patient of symptoms. A patch test to nickel turned out to be positive. After removal of the stainless steel cerclage in the mandible, clinical and radiographic changes returned to normal within 2 and 8 weeks, respectively. It is concluded that, whenever acute treatment does not take priority, patch tests comprising nickel, cobalt and chromium should be performed in patients who are candidates for metal osteosynthesis."}
{"id": "PMID:467028", "title": "Lanolin and derivatives chemistry: relationship to allergic contact dermatitis.", "content": "This paper summarizes lanolin, the composition of its esters, acids and alcohols, its chemical and physical modifications, and refining. It discusses lanolin, its constituents and derivatives, and their relationship to allergic contant dermatitis.", "contents": "Lanolin and derivatives chemistry: relationship to allergic contact dermatitis. This paper summarizes lanolin, the composition of its esters, acids and alcohols, its chemical and physical modifications, and refining. It discusses lanolin, its constituents and derivatives, and their relationship to allergic contant dermatitis."}
{"id": "PMID:467029", "title": "The Duhring chamber. An improved technique for epicutaneous testing of irritant and allergic reactions.", "content": "For assessing skin reactions to irritants and allergens, chambers which confine the test substance to the site of application are much superior to conventional patch tests. Because the concentration and volume do not change appreciably, the method allows for quantification and yields more reproducible results. A new aluminum chamber has been designed which is more useful than its predecessor, the Finn chamber, mainly because of its increased capacity. Data are given to show that the intensity of irritant and allergic reactions is dependent on the quantities applied.", "contents": "The Duhring chamber. An improved technique for epicutaneous testing of irritant and allergic reactions. For assessing skin reactions to irritants and allergens, chambers which confine the test substance to the site of application are much superior to conventional patch tests. Because the concentration and volume do not change appreciably, the method allows for quantification and yields more reproducible results. A new aluminum chamber has been designed which is more useful than its predecessor, the Finn chamber, mainly because of its increased capacity. Data are given to show that the intensity of irritant and allergic reactions is dependent on the quantities applied."}
{"id": "PMID:467030", "title": "Temperature-dependent nickel release from nickel alloys.", "content": "Nickel release from Danish one krone coins and metal buttons from jeans has been measured at 20 degrees C in distilled water and at 35 degrees C in distilled water and synthetic sweat. The temperature elevation increased the nickel release from the coins and two of the nine metal buttons investigated. Obviously the sensitivity to temperature elevation differs from one alloy to another.", "contents": "Temperature-dependent nickel release from nickel alloys. Nickel release from Danish one krone coins and metal buttons from jeans has been measured at 20 degrees C in distilled water and at 35 degrees C in distilled water and synthetic sweat. The temperature elevation increased the nickel release from the coins and two of the nine metal buttons investigated. Obviously the sensitivity to temperature elevation differs from one alloy to another."}
{"id": "PMID:467031", "title": "Isomorphic skin reaction with DNCB in SLE and DLE.", "content": "DNCB sensitization was studied in 76 cases of systemic and 44 of discoid lupus erythematosus and in 101 immunosuppressed and tumor patients as controls, as well as in 40 contact-sensitized eczematous and 115 unselected patients. Sensitization of patients with discoid LE and that of mixed patients are of the same order, while it is lower in the systemic LE group. The highest incidence was found in the polysensitized positive control group; the lowest in the immunosuppressed and tumor patients. DNCB sensitization showed clear age dependency in the different groups. At the site of the DNCB exposure, isomorphic reactions were found in 43% of discoid and 25% of systemic LE patients. Histological and immunofluorescence studies supported the findings of the clinical picture, i. e. the appearance of isomorphic reactions at the site of the exposure cannot be related either to the intensity of the early reaction or with the developing intensity.", "contents": "Isomorphic skin reaction with DNCB in SLE and DLE. DNCB sensitization was studied in 76 cases of systemic and 44 of discoid lupus erythematosus and in 101 immunosuppressed and tumor patients as controls, as well as in 40 contact-sensitized eczematous and 115 unselected patients. Sensitization of patients with discoid LE and that of mixed patients are of the same order, while it is lower in the systemic LE group. The highest incidence was found in the polysensitized positive control group; the lowest in the immunosuppressed and tumor patients. DNCB sensitization showed clear age dependency in the different groups. At the site of the DNCB exposure, isomorphic reactions were found in 43% of discoid and 25% of systemic LE patients. Histological and immunofluorescence studies supported the findings of the clinical picture, i. e. the appearance of isomorphic reactions at the site of the exposure cannot be related either to the intensity of the early reaction or with the developing intensity."}
{"id": "PMID:467032", "title": "Skin sensitivity to denture base materials in the burning mouth syndrome.", "content": "The significance of sensitizing compounds in the denture base for the etiology of the burning mouth syndrome (BMS) has been studied in 53 denture-wearing persons, seven males and 46 females. Epicutaneous patch tests were performed with standard concentrations of benzoyl peroxide, dibutylphthalate, dimethyl-p-toluidine, formaldehyde, hydroquinone, methylmethacrylate, p-phenylendiamine and with cadmium sulfate, potassium dichromate, cobalt chloride and nickel sulfate. Furthermore, patch testing was performed with filings from the denture mixed with the patient's own saliva. In cases with an inflamed oral mucosa, the presence of hyphae of Candida albicans was assessed by a smear technique. Positive skin reactions were observed in 15 persons to dimethyl-p-toluidine, hydroquinone, formaldehyde, methylmethacrylate, p-phenylendiamine, potassium dichromate, cobalt chloride and nickel sulfate, including three cases with reactions to filings from their dentures, and one patient who after subsequent testing showed skin sensitivity to balsam of Peru. In 12 cases an etiological connection could be traced between the oral symptoms and the denture base, indicating that contact sensitivity to base materials or to allergens and microbial antigens on the denture plate plays a greater role in the pathogenesis of BMS in edentulous persons than previously suggested.", "contents": "Skin sensitivity to denture base materials in the burning mouth syndrome. The significance of sensitizing compounds in the denture base for the etiology of the burning mouth syndrome (BMS) has been studied in 53 denture-wearing persons, seven males and 46 females. Epicutaneous patch tests were performed with standard concentrations of benzoyl peroxide, dibutylphthalate, dimethyl-p-toluidine, formaldehyde, hydroquinone, methylmethacrylate, p-phenylendiamine and with cadmium sulfate, potassium dichromate, cobalt chloride and nickel sulfate. Furthermore, patch testing was performed with filings from the denture mixed with the patient's own saliva. In cases with an inflamed oral mucosa, the presence of hyphae of Candida albicans was assessed by a smear technique. Positive skin reactions were observed in 15 persons to dimethyl-p-toluidine, hydroquinone, formaldehyde, methylmethacrylate, p-phenylendiamine, potassium dichromate, cobalt chloride and nickel sulfate, including three cases with reactions to filings from their dentures, and one patient who after subsequent testing showed skin sensitivity to balsam of Peru. In 12 cases an etiological connection could be traced between the oral symptoms and the denture base, indicating that contact sensitivity to base materials or to allergens and microbial antigens on the denture plate plays a greater role in the pathogenesis of BMS in edentulous persons than previously suggested."}
{"id": "PMID:467033", "title": "Allergy to various components of topical preparations in stasis dermatitis and leg ulcer.", "content": "Patients suffering from stasis dermatitis and leg ulcers (192) were found to give an allergic reaction in epicutaneous tests to one or more components of topical preparations in 69.2% of cases. The most common reactions were obtained with neomycin 34.0% and its component framycetin 31.2%, wool alcohols 22.9%, balsam of Peru 21.4%, chlorquinaldon 16.7%, bacitracin 13.1%, chinoform 10.9%, dequalin 9.9%, formaldehyde 4.2% and parabens 3.6%. The incidence of allergic reactions reflects local use of preparations containing various allergens as well as their capacity to produce alllergy. Allergy may in some cases interfere with effective topical therapy. Debrisan dextran polymer beads were found to be suitable for treatment of ulcers of multiallergic patients without risk of contact allergy.", "contents": "Allergy to various components of topical preparations in stasis dermatitis and leg ulcer. Patients suffering from stasis dermatitis and leg ulcers (192) were found to give an allergic reaction in epicutaneous tests to one or more components of topical preparations in 69.2% of cases. The most common reactions were obtained with neomycin 34.0% and its component framycetin 31.2%, wool alcohols 22.9%, balsam of Peru 21.4%, chlorquinaldon 16.7%, bacitracin 13.1%, chinoform 10.9%, dequalin 9.9%, formaldehyde 4.2% and parabens 3.6%. The incidence of allergic reactions reflects local use of preparations containing various allergens as well as their capacity to produce alllergy. Allergy may in some cases interfere with effective topical therapy. Debrisan dextran polymer beads were found to be suitable for treatment of ulcers of multiallergic patients without risk of contact allergy."}
{"id": "PMID:467052", "title": "Hot water as a tubal occluding agent.", "content": "Experimental studies in living rabbits and autopsy human oviducts are presented which demonstrate a reasonable possibility that one could introduce hot water into the proximal portion of the human oviduct with a hysteroscope or balloon catheter, to destroy the epithelium of the first portion of that structure, leading to its occlusion, while still maintaining safety for the woman because the water would cool to tolerable temperatures before it entered the peritoneal cavity. Before trying the procedure in human beings, research on non-human primates is clearly needed.", "contents": "Hot water as a tubal occluding agent. Experimental studies in living rabbits and autopsy human oviducts are presented which demonstrate a reasonable possibility that one could introduce hot water into the proximal portion of the human oviduct with a hysteroscope or balloon catheter, to destroy the epithelium of the first portion of that structure, leading to its occlusion, while still maintaining safety for the woman because the water would cool to tolerable temperatures before it entered the peritoneal cavity. Before trying the procedure in human beings, research on non-human primates is clearly needed."}
{"id": "PMID:467053", "title": "Differences in effect of copper and ProgestasertR IUDS on fibrinolytic activity of the endometrium in the rabbit.", "content": "Rabbits, which have two separate uteri, were used to assess intraindividual differences in the endometrial fibrinolytic activity in the two uteri, one containing a copper IUD and the other a progesterone-releasing IUD (ProgestasertR). A higher activity was demonstrated in the uterus containing the copper-IUD. Enhanced fibrinolytic activity in the endometrium has been held responsible for the increased menstrual blood loss in women using different IUDs. Thus, the present experimental study supports the clinical observation that women using ProgestasertR-IUD have less bleeding complaints than those using copper-IUD.", "contents": "Differences in effect of copper and ProgestasertR IUDS on fibrinolytic activity of the endometrium in the rabbit. Rabbits, which have two separate uteri, were used to assess intraindividual differences in the endometrial fibrinolytic activity in the two uteri, one containing a copper IUD and the other a progesterone-releasing IUD (ProgestasertR). A higher activity was demonstrated in the uterus containing the copper-IUD. Enhanced fibrinolytic activity in the endometrium has been held responsible for the increased menstrual blood loss in women using different IUDs. Thus, the present experimental study supports the clinical observation that women using ProgestasertR-IUD have less bleeding complaints than those using copper-IUD."}
{"id": "PMID:467054", "title": "Timing of the IUD insertion.", "content": "The relationship between the time in the menstrual cycle when a TCu-200 or Cu-7-200 is inserted and subsequent IUD-related events was evaluated. For women who had either TCus or Cu-7s inserted, the pregnancy, expulsion and medical removal rates were similar for insertions performed at any time of the menstrual cycle.", "contents": "Timing of the IUD insertion. The relationship between the time in the menstrual cycle when a TCu-200 or Cu-7-200 is inserted and subsequent IUD-related events was evaluated. For women who had either TCus or Cu-7s inserted, the pregnancy, expulsion and medical removal rates were similar for insertions performed at any time of the menstrual cycle."}
{"id": "PMID:467055", "title": "Termination of pregnancy in the rat by RMI 12,936.", "content": "Administration of a steroid RMI 12,936 (2 MG) ON DAY 8 OF PREGNANCY RESULted in an interruption of gestation within 5 days. No significant changes in progesterone (delta 4P), 20 alpha-dihydroprogesterone (20 alpha-DHP) and pregnenolone (delta 5P) were recorded during the course of termination of pregnancy by RMI 12,936. However, a significant rise in serum delta 4P and hypertropic corpora lutea were evident on day 13 of pregnancy when all treated females showed dead conceptus. The placental tissue was still present at the time of pregnancy termination. On the other hand, serum LH levels was significantly suppressed 24 hr after the treatment and maintained at a very low level. The reduction of LH might be due to a lowered hypophyseal sensitivity towards LH-RH. The occurrence of in utero dead fetus without interference of delta 4P production suggests that in the rat RMI 12,936-induced termination of pregnancy is due to its embryotoxic rather than luteolytic effect. Although the treated females did not return to normal estrous cycles for a period of 2 weeks after pregnancy termination, they were sexually receptive. Successful mating resulted in normal pregnancy which occurred 30-35 days after RMI 12,936 treatment.", "contents": "Termination of pregnancy in the rat by RMI 12,936. Administration of a steroid RMI 12,936 (2 MG) ON DAY 8 OF PREGNANCY RESULted in an interruption of gestation within 5 days. No significant changes in progesterone (delta 4P), 20 alpha-dihydroprogesterone (20 alpha-DHP) and pregnenolone (delta 5P) were recorded during the course of termination of pregnancy by RMI 12,936. However, a significant rise in serum delta 4P and hypertropic corpora lutea were evident on day 13 of pregnancy when all treated females showed dead conceptus. The placental tissue was still present at the time of pregnancy termination. On the other hand, serum LH levels was significantly suppressed 24 hr after the treatment and maintained at a very low level. The reduction of LH might be due to a lowered hypophyseal sensitivity towards LH-RH. The occurrence of in utero dead fetus without interference of delta 4P production suggests that in the rat RMI 12,936-induced termination of pregnancy is due to its embryotoxic rather than luteolytic effect. Although the treated females did not return to normal estrous cycles for a period of 2 weeks after pregnancy termination, they were sexually receptive. Successful mating resulted in normal pregnancy which occurred 30-35 days after RMI 12,936 treatment."}
{"id": "PMID:467056", "title": "Use and acceptance of the \"Paper Pill\" among an urban clinic population.", "content": "A randomized, crossover study comparing the acceptability of two presentations of the same oral contraceptive, a paper formulation and the conventional tablet, was carried out among two-hundred-and-seventy women attending two urban family planning clinics in Mexico City. During the first three cycles of use, the percentage of women discontinuing the medication for side effects was almost identical in the two groups. Although the paper formulation failed to demonstrate any major advantage in terms of continuation or acceptability, it was preferred by a majority of the women who began oral contraception with its use.", "contents": "Use and acceptance of the \"Paper Pill\" among an urban clinic population. A randomized, crossover study comparing the acceptability of two presentations of the same oral contraceptive, a paper formulation and the conventional tablet, was carried out among two-hundred-and-seventy women attending two urban family planning clinics in Mexico City. During the first three cycles of use, the percentage of women discontinuing the medication for side effects was almost identical in the two groups. Although the paper formulation failed to demonstrate any major advantage in terms of continuation or acceptability, it was preferred by a majority of the women who began oral contraception with its use."}
{"id": "PMID:467057", "title": "Low-level, progestogen-releasing vaginal contraceptive devices.", "content": "Silicone rubber vaginal contraceptive devices of four different formulations, which release predetermined, controlled doses of three progestogens at four distinct levels, were designed and fabricated, and tested in 90-day clinical trials. Data obtained with 70 of the devices indicated that in vivo release rates (microgram/day +/- S.D.) for the formulations were: progesterone, 1400 +/- 30; norethindrone (two levels), 49.4 +/- 2.4, 196 +/- 21; d-norgestrel, 21.6 +/- 1.4. Clinical studies with these devices indicate that the women usually ovulate (with the exception of the high-dose norethindrone-releasing devices) while sperm penetration of the cervix was inhibited by all four fromulations, most consistently by the norgestrel-releasing devices.", "contents": "Low-level, progestogen-releasing vaginal contraceptive devices. Silicone rubber vaginal contraceptive devices of four different formulations, which release predetermined, controlled doses of three progestogens at four distinct levels, were designed and fabricated, and tested in 90-day clinical trials. Data obtained with 70 of the devices indicated that in vivo release rates (microgram/day +/- S.D.) for the formulations were: progesterone, 1400 +/- 30; norethindrone (two levels), 49.4 +/- 2.4, 196 +/- 21; d-norgestrel, 21.6 +/- 1.4. Clinical studies with these devices indicate that the women usually ovulate (with the exception of the high-dose norethindrone-releasing devices) while sperm penetration of the cervix was inhibited by all four fromulations, most consistently by the norgestrel-releasing devices."}
{"id": "PMID:467058", "title": "The antifertility activity and toxicity of alpha-chlorohydrin derivatives in male rats.", "content": "The antifertility activity and toxicity of alpha-chlorohydrin, six derivatives and glycidol were investigated in male rats. At a dose of 5 mg/kg injected intraperitoneally each day for 14 days, only alpha-chlorohydrin produced complete infertility. When rats were injected with the derivatives at a dose equivalent to 5 mg/kg of alpha-chlorohydrin on a molar basis, the phosphorylated and amino acid derivatives produced complete infertility within 7 days; however, there were some signs of toxicity. Compound II also produced complete infertility and appeared less toxic. AY - 22,352, compounds III and IV and glycidol had no antifertility activity, although spermatozoan motility was reduced by injection of glycidol.", "contents": "The antifertility activity and toxicity of alpha-chlorohydrin derivatives in male rats. The antifertility activity and toxicity of alpha-chlorohydrin, six derivatives and glycidol were investigated in male rats. At a dose of 5 mg/kg injected intraperitoneally each day for 14 days, only alpha-chlorohydrin produced complete infertility. When rats were injected with the derivatives at a dose equivalent to 5 mg/kg of alpha-chlorohydrin on a molar basis, the phosphorylated and amino acid derivatives produced complete infertility within 7 days; however, there were some signs of toxicity. Compound II also produced complete infertility and appeared less toxic. AY - 22,352, compounds III and IV and glycidol had no antifertility activity, although spermatozoan motility was reduced by injection of glycidol."}
{"id": "PMID:467060", "title": "The role of the interstitium of the renal cortex in renal disease.", "content": "Morphometrical investigations (point-counting method) showed that in different inflammatory (endocapillary -- acute -- GN, mesangioproliferative GN, membranoproliferative GN) glomerulonephritides and in non-inflammatory glomerular diseases (perireticular amyloidosis), there are statistically significant correlation between serum creatine concentrations at the time of biopsy and the enlargement of the cortical interstitium by fibrosis. Similar results were obtained in investigating different grades of benign nephrosclerosis with transition into secondary malignant nephrosclerosis conditioned by hypertension and in chronic diffuse sclerosing interstitial nephritides of different etiologies. As hypothesis, we assume that a narrowing of the postglomerular vessel network by interstitial fibrosis take place. This could lead to an increase resistance of the renal cortical blood flow. In spite of an elevated effective filtration pressure, the slowing of the glomerular blood flow may lead to the reduction of GRF and to an increase of the serum creatinine concentration. Additionally, in the case of interstitial fibrosis the tubules look atrophied. This could be the consequence of the reduced GFR as a sign of inactivity. On the other hand, tubular atrophy could result from malnutrition in the case of interstitial fibrosis. The resorptive capacity of these atrophied-looking tubules could be lowered and the GFR could be diminished by the so-called Thurau mechanism.", "contents": "The role of the interstitium of the renal cortex in renal disease. Morphometrical investigations (point-counting method) showed that in different inflammatory (endocapillary -- acute -- GN, mesangioproliferative GN, membranoproliferative GN) glomerulonephritides and in non-inflammatory glomerular diseases (perireticular amyloidosis), there are statistically significant correlation between serum creatine concentrations at the time of biopsy and the enlargement of the cortical interstitium by fibrosis. Similar results were obtained in investigating different grades of benign nephrosclerosis with transition into secondary malignant nephrosclerosis conditioned by hypertension and in chronic diffuse sclerosing interstitial nephritides of different etiologies. As hypothesis, we assume that a narrowing of the postglomerular vessel network by interstitial fibrosis take place. This could lead to an increase resistance of the renal cortical blood flow. In spite of an elevated effective filtration pressure, the slowing of the glomerular blood flow may lead to the reduction of GRF and to an increase of the serum creatinine concentration. Additionally, in the case of interstitial fibrosis the tubules look atrophied. This could be the consequence of the reduced GFR as a sign of inactivity. On the other hand, tubular atrophy could result from malnutrition in the case of interstitial fibrosis. The resorptive capacity of these atrophied-looking tubules could be lowered and the GFR could be diminished by the so-called Thurau mechanism."}
{"id": "PMID:467061", "title": "Angiographic results in interstitial, vascular and glomerular kidney diseases.", "content": "In earlier stages of parenchymal kidney diseases different changes are to be seen in angiography. No single sign appeared to be pathognomonic for glomerular nephritis, vascular disease or interstitial nephropathy. But the disproportionate frequency of some angiographic features assumed discriminatory value. Morphometry of the renal arteriograms discriminated statistically significant interstitial, vascular and glomerular diseases. The correct angiographical diagnosis was made in 76%.", "contents": "Angiographic results in interstitial, vascular and glomerular kidney diseases. In earlier stages of parenchymal kidney diseases different changes are to be seen in angiography. No single sign appeared to be pathognomonic for glomerular nephritis, vascular disease or interstitial nephropathy. But the disproportionate frequency of some angiographic features assumed discriminatory value. Morphometry of the renal arteriograms discriminated statistically significant interstitial, vascular and glomerular diseases. The correct angiographical diagnosis was made in 76%."}
{"id": "PMID:467065", "title": "Balkan (endemic) nephropathy.", "content": "The clinical and pathological features of Balkan (endemic) nephropathy are discussed and correlations of incidence with excess late summer and autumn rainfall outlined. Cultures of a strain of Penicillium verrucosum var. cyclopium isolated from maize collected in an endemic area were fed to rats and lesions were produced in the straight third segment of the proximal kidney tubules. Extensive degeneration and nuclear changes were seen and on prolonged feeding further nuclear enlargement (to greater than 6n) and the formation of multinucleate cells occurred. The relevance of these findings to the clinical disease in man, especially the occurrence of urinary tract tumours, and the evidence supporting mycotoxin involvement, are discussed.", "contents": "Balkan (endemic) nephropathy. The clinical and pathological features of Balkan (endemic) nephropathy are discussed and correlations of incidence with excess late summer and autumn rainfall outlined. Cultures of a strain of Penicillium verrucosum var. cyclopium isolated from maize collected in an endemic area were fed to rats and lesions were produced in the straight third segment of the proximal kidney tubules. Extensive degeneration and nuclear changes were seen and on prolonged feeding further nuclear enlargement (to greater than 6n) and the formation of multinucleate cells occurred. The relevance of these findings to the clinical disease in man, especially the occurrence of urinary tract tumours, and the evidence supporting mycotoxin involvement, are discussed."}
{"id": "PMID:467074", "title": "The pathogenesis of reflux nephropathy.", "content": "In an experimental study, the scars of chronic pyelonephritis have been shown to be caused by infected intrarenal reflux. Intrarenal reflux occurs in the presence of vesicoureteric reflux and a renal papilla having special morphological characteristics predisposing to this phenomenon. Such papillae occur predominantly in the polar regions of the kidney where large pyelonephritic scars are most commonly seen. The clinical implications of this pathogenic mechanism are discussed.", "contents": "The pathogenesis of reflux nephropathy. In an experimental study, the scars of chronic pyelonephritis have been shown to be caused by infected intrarenal reflux. Intrarenal reflux occurs in the presence of vesicoureteric reflux and a renal papilla having special morphological characteristics predisposing to this phenomenon. Such papillae occur predominantly in the polar regions of the kidney where large pyelonephritic scars are most commonly seen. The clinical implications of this pathogenic mechanism are discussed."}
{"id": "PMID:467076", "title": "Canine viral enteritis. II. Morphologic lesions in naturally occurring parvovirus infection.", "content": "The morphologic lesions associated with naturally occurring canine parvovirus infection are described. The disease is characterized by necrosis of crypt epithelium in the small intestine and of lymphoid tissue in Peyers patches, lymph nodes, spleen and thymus.", "contents": "Canine viral enteritis. II. Morphologic lesions in naturally occurring parvovirus infection. The morphologic lesions associated with naturally occurring canine parvovirus infection are described. The disease is characterized by necrosis of crypt epithelium in the small intestine and of lymphoid tissue in Peyers patches, lymph nodes, spleen and thymus."}
{"id": "PMID:467077", "title": "Dermatohistopathologic changes in bovine congenital porphyria.", "content": "Congenital porphyria was diagnosed in 4 crossbred cattle. All had pink-brown discoloration of the teeth and amber colored urine, which fluoresced bright orange with exposure to ultraviolet light. Elevated levels of uroporphyrin and coproporphyrin were found in the urine of all 4 cattle. Skin biopsies taken from 2 of the cattle revealed subepidermal blister formation and thickening of upper dermal blood vessel walls with deposition of a PAS-positive, hyaline-like material.", "contents": "Dermatohistopathologic changes in bovine congenital porphyria. Congenital porphyria was diagnosed in 4 crossbred cattle. All had pink-brown discoloration of the teeth and amber colored urine, which fluoresced bright orange with exposure to ultraviolet light. Elevated levels of uroporphyrin and coproporphyrin were found in the urine of all 4 cattle. Skin biopsies taken from 2 of the cattle revealed subepidermal blister formation and thickening of upper dermal blood vessel walls with deposition of a PAS-positive, hyaline-like material."}
{"id": "PMID:467078", "title": "Besnoitiosis in wild and domestic goats in Iran.", "content": "Besnoitia cysts and lesions were observed in the skin, blood vessels, epididymis and testes of two wild goats (Capra aegagrus) and in the subcutaneous tissues of two domestic goats (Capra hircus) in the Fars Province of Iran. The seminiferous tubules in the wild goats showed aspermatogenesis, degeneration and atrophy of germinal epithelium.", "contents": "Besnoitiosis in wild and domestic goats in Iran. Besnoitia cysts and lesions were observed in the skin, blood vessels, epididymis and testes of two wild goats (Capra aegagrus) and in the subcutaneous tissues of two domestic goats (Capra hircus) in the Fars Province of Iran. The seminiferous tubules in the wild goats showed aspermatogenesis, degeneration and atrophy of germinal epithelium."}
{"id": "PMID:467079", "title": "Embryotoxic effects of pine needles and pine needle extracts.", "content": "A heat stable toxin present in needles of ponderosa pine was found to be soluble in methanol, ethanol, chloroform hexanes and 1-butanol. The embryotoxic effects of fresh green pine needles and a chloroform/methanol extract were determined by measuring embryo resorption in pregnant mice. Autoclaving the needles and extract for 1 hour prior to feeding enhanced the embryoresorptive effect by 28% and 32%, respectively. The results of this study revealed that the embryo resorptive dose (ERD50) of heat stable toxin for 1 mouse was 8.95 gms. for fresh green pine needles and 6.46 gms. for autoclaved green pine needles. In addition to embryocidal effects, feeding of the toxin resulted in significant weight loss in adult mice.", "contents": "Embryotoxic effects of pine needles and pine needle extracts. A heat stable toxin present in needles of ponderosa pine was found to be soluble in methanol, ethanol, chloroform hexanes and 1-butanol. The embryotoxic effects of fresh green pine needles and a chloroform/methanol extract were determined by measuring embryo resorption in pregnant mice. Autoclaving the needles and extract for 1 hour prior to feeding enhanced the embryoresorptive effect by 28% and 32%, respectively. The results of this study revealed that the embryo resorptive dose (ERD50) of heat stable toxin for 1 mouse was 8.95 gms. for fresh green pine needles and 6.46 gms. for autoclaved green pine needles. In addition to embryocidal effects, feeding of the toxin resulted in significant weight loss in adult mice."}
{"id": "PMID:467080", "title": "Lymphoreticular neoplasia in a dog resembling malignant histiocytosis (histiocytic medullary reticulosis) in man.", "content": "Malignant histiocytosis was diagnosed in a 4-year-old-dog. Major clinicopathologic findings included multiple cutaneous tumors, peripheral lymphadenopathy, hepatomegaly, lytic bone lesions, anemia, thrombocytopenia, and monoclonal IgA gammopathy. Pathologic findings included multi-systemic infiltration of erythrophagocytic malignant histiocytes. Chemotherapy was unsuccessful.", "contents": "Lymphoreticular neoplasia in a dog resembling malignant histiocytosis (histiocytic medullary reticulosis) in man. Malignant histiocytosis was diagnosed in a 4-year-old-dog. Major clinicopathologic findings included multiple cutaneous tumors, peripheral lymphadenopathy, hepatomegaly, lytic bone lesions, anemia, thrombocytopenia, and monoclonal IgA gammopathy. Pathologic findings included multi-systemic infiltration of erythrophagocytic malignant histiocytes. Chemotherapy was unsuccessful."}
{"id": "PMID:467082", "title": "Industrial fluoride pollution. Chronic fluoride poisoning in Cornwall Island cattle.", "content": "An aluminum plant on the south bank of the St. Lawrence river, southwest of Cornwall Island, Ontario, Canada, has emitted 0.816 metric tons of fluoride daily since 1973; considerably higher amounts were emitted from 1959 to 1973. The plant has been designated as the \"major source of fluoride emissions impacting on Cornwall Island.\" Chronic fluoride poisoning in Cornwall island cattle was manifested clinically by stunted growth and dental fluorosis to a degree of severe interference with drinking and mastication. Cows died at or were slaughtered after the third pregnancy. The deterioration of cows did not allow further pregnancies. Fluoride concentrations in ash of biopsied coccygeal vertebrae increased significantly with age and were dependent on distance from and direction to the aluminum plant. Fluoride in bone ash of a 7-month old-fetus exceeded 500 ppm; fluoride thus was passed transplacentally. Analyses of fluoride in ash of bones obtained at necropsy of cattle from 4 months of age to 4 to 5 years of age showed increased amounts with age. Cancellous bone retained far higher amounts than cortical bone, a reflection of the normally higher metabolic rate of cancellous bone. Concentrations exceeding 10,000 ppm fluoride were recorded in cancellous bone of a 4-to 5-year-old cow. The target cells for fluoride in chronic fluorosis were shown to be the ameloblasts, the dental pulp cells and the odontoblasts and, in bone, primarily the resorbing osteocytes and also the osteoblasts. Atrophy and necrosis of the ameloblasts were responsible for enamel defects. The existing enamel showed brown discoloration from fluoride deposits. The pulp cells underwent fibrous and osseous metaplasia and necrosis of the ectopic bone occurred. The odontoblasts were atrophic and the dentin showed brown discoloration. The resorbing osteocytes were inactive and osteosclerosis resulted. This was especially pronounced in areas of normally great apposition, i.e. in the metaphyses. The epiphyseal plate became squeezed between petrotic bone and growth was stunted. Resorption of alveolar bone surrounding the deciduous teeth was severely retarded or arrested. A delay in eruption of permanent teeth occurred; it was up to 3.5 years in incisor teeth. Interference with the resorbing osteocytes in fluorotic bone was also demonstrated by loss of collagen birefringency in such bone. Failure of bone resorption also caused retention of trabecular bone in the cortices; this was observed even in a 4-t0-5-year-old cow. In areas where modeling into osteonic bone had begun, fluoride deposits were extremely heavy but this bone showed numerous soft osteons in microradiographs. The toxic effect of fluoride on osteocytes also resulted in the death of the cells. Such osteonecrosis occurred mainly in gnathic bone. There was atrophy of the osteoblasts. Osteopenia thus resulted from osteonecrosis and osteoporosis. Subperiosteal exostoses were not observed in long bones. The degree of fluorosis in Cornwall Island cattle was severe...", "contents": "Industrial fluoride pollution. Chronic fluoride poisoning in Cornwall Island cattle. An aluminum plant on the south bank of the St. Lawrence river, southwest of Cornwall Island, Ontario, Canada, has emitted 0.816 metric tons of fluoride daily since 1973; considerably higher amounts were emitted from 1959 to 1973. The plant has been designated as the \"major source of fluoride emissions impacting on Cornwall Island.\" Chronic fluoride poisoning in Cornwall island cattle was manifested clinically by stunted growth and dental fluorosis to a degree of severe interference with drinking and mastication. Cows died at or were slaughtered after the third pregnancy. The deterioration of cows did not allow further pregnancies. Fluoride concentrations in ash of biopsied coccygeal vertebrae increased significantly with age and were dependent on distance from and direction to the aluminum plant. Fluoride in bone ash of a 7-month old-fetus exceeded 500 ppm; fluoride thus was passed transplacentally. Analyses of fluoride in ash of bones obtained at necropsy of cattle from 4 months of age to 4 to 5 years of age showed increased amounts with age. Cancellous bone retained far higher amounts than cortical bone, a reflection of the normally higher metabolic rate of cancellous bone. Concentrations exceeding 10,000 ppm fluoride were recorded in cancellous bone of a 4-to 5-year-old cow. The target cells for fluoride in chronic fluorosis were shown to be the ameloblasts, the dental pulp cells and the odontoblasts and, in bone, primarily the resorbing osteocytes and also the osteoblasts. Atrophy and necrosis of the ameloblasts were responsible for enamel defects. The existing enamel showed brown discoloration from fluoride deposits. The pulp cells underwent fibrous and osseous metaplasia and necrosis of the ectopic bone occurred. The odontoblasts were atrophic and the dentin showed brown discoloration. The resorbing osteocytes were inactive and osteosclerosis resulted. This was especially pronounced in areas of normally great apposition, i.e. in the metaphyses. The epiphyseal plate became squeezed between petrotic bone and growth was stunted. Resorption of alveolar bone surrounding the deciduous teeth was severely retarded or arrested. A delay in eruption of permanent teeth occurred; it was up to 3.5 years in incisor teeth. Interference with the resorbing osteocytes in fluorotic bone was also demonstrated by loss of collagen birefringency in such bone. Failure of bone resorption also caused retention of trabecular bone in the cortices; this was observed even in a 4-t0-5-year-old cow. In areas where modeling into osteonic bone had begun, fluoride deposits were extremely heavy but this bone showed numerous soft osteons in microradiographs. The toxic effect of fluoride on osteocytes also resulted in the death of the cells. Such osteonecrosis occurred mainly in gnathic bone. There was atrophy of the osteoblasts. Osteopenia thus resulted from osteonecrosis and osteoporosis. Subperiosteal exostoses were not observed in long bones. The degree of fluorosis in Cornwall Island cattle was severe..."}
{"id": "PMID:467083", "title": "The Trendelenburg position: hemodynamic effects in hypotensive and normotensive patients.", "content": "The effect of the Trendelenburg position on systemic and pulmonary hemodynamics in critically ill patients is not generally appreciated. This study examined the hemodynamoc effect of 15-20 degrees head-down tilt in 61 normotensive and 15 hypotensive patients with acute cardiac illness or sepsis. In normotensive patients, the head-down tilt increased the preload of both right and left ventricles, increased cardiac output slightly, decreased systemic vascular resistance, and did not change the mean arterial pressure. This effect was probably mediated by baroreceptor stimulation. In hypotensive patients, the Trendelenburg position did not increase preload, slightly increased afterload, and decreased cardiac output. This study failed to document any beneficial hemodynamic effect of the Trendelenburg position in critically ill normo- or hypotensive patients.", "contents": "The Trendelenburg position: hemodynamic effects in hypotensive and normotensive patients. The effect of the Trendelenburg position on systemic and pulmonary hemodynamics in critically ill patients is not generally appreciated. This study examined the hemodynamoc effect of 15-20 degrees head-down tilt in 61 normotensive and 15 hypotensive patients with acute cardiac illness or sepsis. In normotensive patients, the head-down tilt increased the preload of both right and left ventricles, increased cardiac output slightly, decreased systemic vascular resistance, and did not change the mean arterial pressure. This effect was probably mediated by baroreceptor stimulation. In hypotensive patients, the Trendelenburg position did not increase preload, slightly increased afterload, and decreased cardiac output. This study failed to document any beneficial hemodynamic effect of the Trendelenburg position in critically ill normo- or hypotensive patients."}
{"id": "PMID:467085", "title": "Partial obstruction of endotracheal tubes in children: incidence, etiology, significance.", "content": "We examined 99 endotracheal tubes removed from 81 pediatric patients to determine the incidence of partial endotracheal tube obstruction and predisposing factors. The overall incidence of obstruction was 20.2% (20/99); 14 of the 20 obstructed tubes were occluded less than 50%. General anesthesia during the period of intubation was the only factor significantly associated with an increased incidence of obstruction (p less than 0.05). There was no correlation of obstruction with duration of intubation, the presence of a Murphy-eye side hole, or small tube size. Among the 71 tubes from patients who received general anesthesia, a tube size of 4 mm or less was associated with an increased incidence of obstruction (p less than 0.05). Pressure-flow curves for three different sizes of endotracheal tubes were constructed to determine the increase in resistance produced by different degrees of obstruction. At flow rates that approximated those found in children during quiet breathing, the resistance of tubes that were 50% obstructed was 4 times greater than that found in unobstructed tubes.", "contents": "Partial obstruction of endotracheal tubes in children: incidence, etiology, significance. We examined 99 endotracheal tubes removed from 81 pediatric patients to determine the incidence of partial endotracheal tube obstruction and predisposing factors. The overall incidence of obstruction was 20.2% (20/99); 14 of the 20 obstructed tubes were occluded less than 50%. General anesthesia during the period of intubation was the only factor significantly associated with an increased incidence of obstruction (p less than 0.05). There was no correlation of obstruction with duration of intubation, the presence of a Murphy-eye side hole, or small tube size. Among the 71 tubes from patients who received general anesthesia, a tube size of 4 mm or less was associated with an increased incidence of obstruction (p less than 0.05). Pressure-flow curves for three different sizes of endotracheal tubes were constructed to determine the increase in resistance produced by different degrees of obstruction. At flow rates that approximated those found in children during quiet breathing, the resistance of tubes that were 50% obstructed was 4 times greater than that found in unobstructed tubes."}
{"id": "PMID:467087", "title": "Physiological significance of oxygen dissociation curve shifts.", "content": "The oxygen delivery system consists of multiple components balanced to provide optimal oxygen supply to all tissues and arranged such that an abnormality in one component is compensated by adjustments in others. Analysis of pertinent data indicates that leftward shifts of the oxygen dissociation curve (ODC) in normal animals and persons elicit adaptive changes in other components which sustain normal oxygen supply. By contrast, several recent studied indicate that leftward ODC shifts, when occurring in combination with other abnormalities of oxygen delivery, produce or aggravate hypoxia. These data suggest that the brain and heart are the organs particularly at risk. It is concluded that leftward ODC shifts, due to massive transfusion of stored blood and to hyperventilation of unconscious patients, are most likely to be hazardous in the presence of poor perfusion, coronary artery disease, or cerebrovascular disease.", "contents": "Physiological significance of oxygen dissociation curve shifts. The oxygen delivery system consists of multiple components balanced to provide optimal oxygen supply to all tissues and arranged such that an abnormality in one component is compensated by adjustments in others. Analysis of pertinent data indicates that leftward shifts of the oxygen dissociation curve (ODC) in normal animals and persons elicit adaptive changes in other components which sustain normal oxygen supply. By contrast, several recent studied indicate that leftward ODC shifts, when occurring in combination with other abnormalities of oxygen delivery, produce or aggravate hypoxia. These data suggest that the brain and heart are the organs particularly at risk. It is concluded that leftward ODC shifts, due to massive transfusion of stored blood and to hyperventilation of unconscious patients, are most likely to be hazardous in the presence of poor perfusion, coronary artery disease, or cerebrovascular disease."}
{"id": "PMID:467089", "title": "Plasma and saliva concentrations for a new formulation of erythromycin stearate.", "content": "Erythromycin saliva and plasma concentrations were determined in 10 subjects after the first and fourth doses of a new formulation of erythromycin stearate ('Erythrocin'), 500 mg 8-hourly, taken immediately before food. Consistent absorption occurred and the steady state plasma levels compared favourably with the minimal inhibitory concentrations of erythromycin for common respiratory pathogens. There was a significant positive correlation between steady state saliva and plasma levels.", "contents": "Plasma and saliva concentrations for a new formulation of erythromycin stearate. Erythromycin saliva and plasma concentrations were determined in 10 subjects after the first and fourth doses of a new formulation of erythromycin stearate ('Erythrocin'), 500 mg 8-hourly, taken immediately before food. Consistent absorption occurred and the steady state plasma levels compared favourably with the minimal inhibitory concentrations of erythromycin for common respiratory pathogens. There was a significant positive correlation between steady state saliva and plasma levels."}
{"id": "PMID:467090", "title": "Indoprofen, a new non-steroidal anti-inflammatory drug, in the treatment of osteoarthrosis: report on a multi-centre study in 1629 patients.", "content": "A multi-centre trial was carried out in 1629 patients with osteoarthrosis in 233 clinical centres to investigate the therapeutic efficacy and tolerability of indoprofen in this clinical condition. The results from assessments of both subjective and objective variables were comparable to those obtained in Phase 3 studies. Moreover, 87.2% of patients expressed a 'good' or 'very good' opinion on the product's efficacy after treatment, usually with 3 tablets (600 mg) daily for 4 weeks. The benefit/risk relationship was confirmed as very favourable on account of the marked activity of the drug and the acceptable frequency of adverse reactions, which were mainly subjective and slight and confined to the gastro-intestinal tract.", "contents": "Indoprofen, a new non-steroidal anti-inflammatory drug, in the treatment of osteoarthrosis: report on a multi-centre study in 1629 patients. A multi-centre trial was carried out in 1629 patients with osteoarthrosis in 233 clinical centres to investigate the therapeutic efficacy and tolerability of indoprofen in this clinical condition. The results from assessments of both subjective and objective variables were comparable to those obtained in Phase 3 studies. Moreover, 87.2% of patients expressed a 'good' or 'very good' opinion on the product's efficacy after treatment, usually with 3 tablets (600 mg) daily for 4 weeks. The benefit/risk relationship was confirmed as very favourable on account of the marked activity of the drug and the acceptable frequency of adverse reactions, which were mainly subjective and slight and confined to the gastro-intestinal tract."}
{"id": "PMID:467091", "title": "An evaluation of a post-operative vacuum drainage system.", "content": "The effectiveness of a plastic bottle vacuum drainage system ('Steritex vacu-drain') was evaluated in 50 randomly selected patients undergoing major surgery. The incidence of post-operative wound infection was 6%. The drainage system was found to be effective and acceptable to both patients and personnel involved in its management.", "contents": "An evaluation of a post-operative vacuum drainage system. The effectiveness of a plastic bottle vacuum drainage system ('Steritex vacu-drain') was evaluated in 50 randomly selected patients undergoing major surgery. The incidence of post-operative wound infection was 6%. The drainage system was found to be effective and acceptable to both patients and personnel involved in its management."}
{"id": "PMID:467092", "title": "An evaluation of the bile acid binding and antacid properties of hydrotalcite in hiatus hernia and peptic ulceration.", "content": "The bile acid binding and antacid properties of hydrotalcite were studied in 25 patients with hiatus hernia or peptic ulceration. Hydrotalcite was found to have significant bile acid binding properties in the presence of free acid in the stomach. There was a definite antacid effect in both pathological entities. These results suggest that hydrotalcite may be an effective therapeutic agent in upper gastro-intestinal ulceration, warranting further investigation in lesions which are thought to involve both hydrochloric acid and bile acids in their pathogenesis.", "contents": "An evaluation of the bile acid binding and antacid properties of hydrotalcite in hiatus hernia and peptic ulceration. The bile acid binding and antacid properties of hydrotalcite were studied in 25 patients with hiatus hernia or peptic ulceration. Hydrotalcite was found to have significant bile acid binding properties in the presence of free acid in the stomach. There was a definite antacid effect in both pathological entities. These results suggest that hydrotalcite may be an effective therapeutic agent in upper gastro-intestinal ulceration, warranting further investigation in lesions which are thought to involve both hydrochloric acid and bile acids in their pathogenesis."}
{"id": "PMID:467093", "title": "Short-term topical treatment of vulvovaginal candidiasis with the combination of 5-fluorocytosine and candicidin.", "content": "A combination of 5-fluorocytosine (1000 mg) and candicidin (5 mg) was compared with clotrimazole (100 mg) in the treatment of 112 non-pregnant volunteers with vulvovaginal candidiasis. Seventy-two (75%) of the 96 patients treated with 1 or 2 vaginal tablets of the combination product and 10 (62%) of the 16 women treated with clotrimazole for 6 days were cured. The efficacy of the treatments was assessed mycologically and clinically 1 week and 1 month after the therapy. Both treatments were well tolerated and no side-effects could be detected. It is concluded that a short-term vaginal treatment with a 5-fluorocytosine and candicidin combination is effective in vaginal moniliasis, and the brevity of the treatment is an advantage compared with others now available.", "contents": "Short-term topical treatment of vulvovaginal candidiasis with the combination of 5-fluorocytosine and candicidin. A combination of 5-fluorocytosine (1000 mg) and candicidin (5 mg) was compared with clotrimazole (100 mg) in the treatment of 112 non-pregnant volunteers with vulvovaginal candidiasis. Seventy-two (75%) of the 96 patients treated with 1 or 2 vaginal tablets of the combination product and 10 (62%) of the 16 women treated with clotrimazole for 6 days were cured. The efficacy of the treatments was assessed mycologically and clinically 1 week and 1 month after the therapy. Both treatments were well tolerated and no side-effects could be detected. It is concluded that a short-term vaginal treatment with a 5-fluorocytosine and candicidin combination is effective in vaginal moniliasis, and the brevity of the treatment is an advantage compared with others now available."}
{"id": "PMID:467095", "title": "Hormone relationships in breast cancer: the role of receptor-binding proteins.", "content": "The introduction of receptor protein studies as markers for hormone dependence of breast carcinomas has had a profound effect on the management of advanced disease. All breast carcinomas, regardless of stage, should have receptor binding protein studies for estrogen and progesterone by the sucrose gradient method or by the dextran-coated charcoal method as a good alternative. A simpler test is needed. In cases of primary advanced disease or in recurrent disease, receptor binding protein studies effectively identify the subgroup of hormone-dependent tumors that should be managed by surgical hormone ablation or endocrine manipulation. These tests are even more effective in identifying patients that should not receive hormone therapy and respond better to chemotherapy. In primary, potentially surgically curable disease, receptor studies may serve as a therapeutic guide to adjuvant hormone or chemotherapy. In all breast cancers, ER and PgR receptor studies should be performed and the information should be stored for possible future use. If possible, tisssue should be stored in a freezer in anticipation of future tests and for confirmation of hormone dependence in the event of recurrence.", "contents": "Hormone relationships in breast cancer: the role of receptor-binding proteins. The introduction of receptor protein studies as markers for hormone dependence of breast carcinomas has had a profound effect on the management of advanced disease. All breast carcinomas, regardless of stage, should have receptor binding protein studies for estrogen and progesterone by the sucrose gradient method or by the dextran-coated charcoal method as a good alternative. A simpler test is needed. In cases of primary advanced disease or in recurrent disease, receptor binding protein studies effectively identify the subgroup of hormone-dependent tumors that should be managed by surgical hormone ablation or endocrine manipulation. These tests are even more effective in identifying patients that should not receive hormone therapy and respond better to chemotherapy. In primary, potentially surgically curable disease, receptor studies may serve as a therapeutic guide to adjuvant hormone or chemotherapy. In all breast cancers, ER and PgR receptor studies should be performed and the information should be stored for possible future use. If possible, tisssue should be stored in a freezer in anticipation of future tests and for confirmation of hormone dependence in the event of recurrence."}
{"id": "PMID:467098", "title": "Lichen sclerosus et atrophicus generalisata, alopecia areata, and polymyalgia rheumatica found in the same patient.", "content": "A sixty-eight year old woman with lichen sclerosus et atrophicus since the age of fity-two, alopecia totalis since the age of thirty-six, and polymyalgia rheumatica since the age of fifty-eight is presented. Besides alopecia totalis, the patient has typical lichen sclerosus et atrophicus changes on the trunk, upper arms, and thighs, and at the first visit an ulcer on her left breast. An immunologic link between her three diseases is suspected. As shown in this case Wood's light is of great value in depigmented skin changes.", "contents": "Lichen sclerosus et atrophicus generalisata, alopecia areata, and polymyalgia rheumatica found in the same patient. A sixty-eight year old woman with lichen sclerosus et atrophicus since the age of fity-two, alopecia totalis since the age of thirty-six, and polymyalgia rheumatica since the age of fifty-eight is presented. Besides alopecia totalis, the patient has typical lichen sclerosus et atrophicus changes on the trunk, upper arms, and thighs, and at the first visit an ulcer on her left breast. An immunologic link between her three diseases is suspected. As shown in this case Wood's light is of great value in depigmented skin changes."}
{"id": "PMID:467099", "title": "Solar urticaria. Report of an unusual case.", "content": "Solar urticaria is a relatively rare dermatosis. In reported cases, skin lesions appeared soon after sunlight irradiation, usually within a few minutes, and consisted of a wheal localized to the exposed area with a surrounding flare. The case presented herein is quite different from the others in its clinical manifestation. The time required for the formation of the wheal was far longer and there was neither surrounding flare nor pseudopodia. Also, the degree of local edema was directly proportional to the length of exposure time, up to three hours. A diagnosis of solar urticaria had not previously been made in this patient because he did not show wheal formation except after unusually long exposure.", "contents": "Solar urticaria. Report of an unusual case. Solar urticaria is a relatively rare dermatosis. In reported cases, skin lesions appeared soon after sunlight irradiation, usually within a few minutes, and consisted of a wheal localized to the exposed area with a surrounding flare. The case presented herein is quite different from the others in its clinical manifestation. The time required for the formation of the wheal was far longer and there was neither surrounding flare nor pseudopodia. Also, the degree of local edema was directly proportional to the length of exposure time, up to three hours. A diagnosis of solar urticaria had not previously been made in this patient because he did not show wheal formation except after unusually long exposure."}
{"id": "PMID:467100", "title": "Pseudofolliculitis--revised concepts of diagnosis and treatment. Report of three cases in women.", "content": "Three cases of pseudofolliculitis are described in black American women, bringing the total number of cases described in the literature to five. Pseudofolliculitis pubis, pseudofolliculitis axillae, and pseudofolliculitis faciale (for women with facial involvement) are new diagnoses we propose to add to the nomenclature. Topical retinoic acid may be the treatment of choice. It appears that this is not a rare disease in black women.", "contents": "Pseudofolliculitis--revised concepts of diagnosis and treatment. Report of three cases in women. Three cases of pseudofolliculitis are described in black American women, bringing the total number of cases described in the literature to five. Pseudofolliculitis pubis, pseudofolliculitis axillae, and pseudofolliculitis faciale (for women with facial involvement) are new diagnoses we propose to add to the nomenclature. Topical retinoic acid may be the treatment of choice. It appears that this is not a rare disease in black women."}
{"id": "PMID:467101", "title": "Bowen's disease of the plantar arch.", "content": "Bowen's disease has previously been associated with the development of other malignancies particularly in patients with a history of arsenic exposure. A review of the literature is combined with a discussion of current theories regarding arsenic exposure, lesion differentiation, and prognostic implications. This report of a bowenoid lesion of the plantar arch emphasizes the importance of close follow-up of patients with Bowen's disease, especially on unexposed surfaces.", "contents": "Bowen's disease of the plantar arch. Bowen's disease has previously been associated with the development of other malignancies particularly in patients with a history of arsenic exposure. A review of the literature is combined with a discussion of current theories regarding arsenic exposure, lesion differentiation, and prognostic implications. This report of a bowenoid lesion of the plantar arch emphasizes the importance of close follow-up of patients with Bowen's disease, especially on unexposed surfaces."}
{"id": "PMID:467102", "title": "A cure for recurrent furunculosis.", "content": "A therapy for recurrent boils using antibiotic ointment over the entire integument is described herein. Rather than eradicating the individual nidus of infection, the skin is allowed to muster its own defenses, resulting in fewer, and eventually no, eruptions.", "contents": "A cure for recurrent furunculosis. A therapy for recurrent boils using antibiotic ointment over the entire integument is described herein. Rather than eradicating the individual nidus of infection, the skin is allowed to muster its own defenses, resulting in fewer, and eventually no, eruptions."}
{"id": "PMID:467103", "title": "Human cutaneous topography a new photographic technique: observations on normal skin.", "content": "We describe herein a convenient and inexpensive technique for photographic examination of the surface of the skin. With this method, the fine lines of the skin appear as furrows or folds. The furrows demarcate units of epidermal structure which we have designated polyangular units. These, in turn, are arranged in a mosaic pattern. This technique was used to define the topography of normal young adults. Surface features fall into four major patterns: 1) dermatoglyphic, 2) hair bearing, 3) frictional, and 4) flexural.", "contents": "Human cutaneous topography a new photographic technique: observations on normal skin. We describe herein a convenient and inexpensive technique for photographic examination of the surface of the skin. With this method, the fine lines of the skin appear as furrows or folds. The furrows demarcate units of epidermal structure which we have designated polyangular units. These, in turn, are arranged in a mosaic pattern. This technique was used to define the topography of normal young adults. Surface features fall into four major patterns: 1) dermatoglyphic, 2) hair bearing, 3) frictional, and 4) flexural."}
{"id": "PMID:467104", "title": "Minocycline therapy of aquarium granuloma. Case reports and literature review.", "content": "Two patients with Mycobacterium marinum infections from home tropical fish aquariums are presented herein. Both patients showed dramatic improvement with minocycline therapy. In vitro susceptibility results also confirmed minocycline, doxycycline, and tetracycline efficacy using disc diffusion and dilution methods.", "contents": "Minocycline therapy of aquarium granuloma. Case reports and literature review. Two patients with Mycobacterium marinum infections from home tropical fish aquariums are presented herein. Both patients showed dramatic improvement with minocycline therapy. In vitro susceptibility results also confirmed minocycline, doxycycline, and tetracycline efficacy using disc diffusion and dilution methods."}
{"id": "PMID:467105", "title": "Paper jumpsuit: an adjunct for psoriasis therapy.", "content": "An inexpensive, disposable, durable paper jumpsuit is described as an adjunct to inpatient or outpatient psoriasis therapy.", "contents": "Paper jumpsuit: an adjunct for psoriasis therapy. An inexpensive, disposable, durable paper jumpsuit is described as an adjunct to inpatient or outpatient psoriasis therapy."}
{"id": "PMID:467109", "title": "Segmental pulmonary angiography in the critically-ill patient using a flow-directed catheter.", "content": "Selective pulmonary angiography was performed using a flow-directed, balloon-tipped catheter in 20 consecutive intensive care unit patients requiring heart catheterizations on the right side for hemodynamic monitoring. No morbidity was encountered from this procedure. Adequate quality balloon-occlusion angiographs were obtained with a portable chest roentgenogram in 17 (85 percent) but appeared normal in only 12 (60 percent), seriously limiting the usefulness of this technique in the diagnosis of occult pulmonary embolisms. With this technique, the catheter tip was found to be in a segmental pulmonary artery anterior to the left atrium (zone I) in 30 percent of the patients. From these findings, we conclude that not infrequently the catheter tip was situated in zone I anterior to the left atriums. Slective pulmonary angiography using a flow-directed, balloon-tipped catheter proved useful in demonstrating the precise location of the catheter tip. When this is the case, the catheter tip should be relocated to a segment below the left atrium (zone III) in patients requiring positive end-expiratory pressure because the \"wedge\" pressure measured in zone I may not accurately reflect left atrial pressure.", "contents": "Segmental pulmonary angiography in the critically-ill patient using a flow-directed catheter. Selective pulmonary angiography was performed using a flow-directed, balloon-tipped catheter in 20 consecutive intensive care unit patients requiring heart catheterizations on the right side for hemodynamic monitoring. No morbidity was encountered from this procedure. Adequate quality balloon-occlusion angiographs were obtained with a portable chest roentgenogram in 17 (85 percent) but appeared normal in only 12 (60 percent), seriously limiting the usefulness of this technique in the diagnosis of occult pulmonary embolisms. With this technique, the catheter tip was found to be in a segmental pulmonary artery anterior to the left atrium (zone I) in 30 percent of the patients. From these findings, we conclude that not infrequently the catheter tip was situated in zone I anterior to the left atriums. Slective pulmonary angiography using a flow-directed, balloon-tipped catheter proved useful in demonstrating the precise location of the catheter tip. When this is the case, the catheter tip should be relocated to a segment below the left atrium (zone III) in patients requiring positive end-expiratory pressure because the \"wedge\" pressure measured in zone I may not accurately reflect left atrial pressure."}
{"id": "PMID:467110", "title": "Radionuclide scintiphotography in defining postoperative pulmonary vasoconstriction. Succesful results after tolazoline administration in a four-month-old infant with congenital heart disease.", "content": "99mTechnetium macroaggregated albumin has successfully been used to define severe postoperative pulmonary vasoconstriction in a four-month-old boy with D-transposition of the great vessels who had undergone a Blalock-Hanlon surgical atrial septectomy. Radionuclide imaging documented clinically suspected pulmonary vasoconstriction and led to the successful use of tolazoline (Priscoline) to reverse the vasoconstriction with improved pulmonary blood flow patterns.", "contents": "Radionuclide scintiphotography in defining postoperative pulmonary vasoconstriction. Succesful results after tolazoline administration in a four-month-old infant with congenital heart disease. 99mTechnetium macroaggregated albumin has successfully been used to define severe postoperative pulmonary vasoconstriction in a four-month-old boy with D-transposition of the great vessels who had undergone a Blalock-Hanlon surgical atrial septectomy. Radionuclide imaging documented clinically suspected pulmonary vasoconstriction and led to the successful use of tolazoline (Priscoline) to reverse the vasoconstriction with improved pulmonary blood flow patterns."}
{"id": "PMID:467113", "title": "Does abdominal breathing affect regional gas exchange?", "content": "Does the use of the abdomen during inspiration alter gas exchange to the bases of the lungs? Most previous investigators have concluded that this technique does not affect regional ventilation. However, if gas concentrations are used as an index of gas exchange, if the subject has normal lungs, is seated and performs the maneuver with the assistance of a physiotherapist, the answer is an unequivocal yes. This study does not pinpoint the exact mechanism by which this alteration is accomplished, but suggests that changes in thoracoabdominal shape influence regional blood flow. It does not indicate whether this effect is beneficial or detrimental. Whether the large number of patients with obstructive lung disease who attempt this maneuver in an unsupervised manner alter gas exchange in any way is uncertain. However, the data do suggest that some patients might be affecting their lung function, and we should learn more about the precise mechanisms.", "contents": "Does abdominal breathing affect regional gas exchange? Does the use of the abdomen during inspiration alter gas exchange to the bases of the lungs? Most previous investigators have concluded that this technique does not affect regional ventilation. However, if gas concentrations are used as an index of gas exchange, if the subject has normal lungs, is seated and performs the maneuver with the assistance of a physiotherapist, the answer is an unequivocal yes. This study does not pinpoint the exact mechanism by which this alteration is accomplished, but suggests that changes in thoracoabdominal shape influence regional blood flow. It does not indicate whether this effect is beneficial or detrimental. Whether the large number of patients with obstructive lung disease who attempt this maneuver in an unsupervised manner alter gas exchange in any way is uncertain. However, the data do suggest that some patients might be affecting their lung function, and we should learn more about the precise mechanisms."}
{"id": "PMID:467114", "title": "Ventilation response and drive during hypoxia in adult patients with asthma.", "content": "We studied ventilation and inspiratory muscle activity during progressive isocapnic hypoxia in adult asthmatic patients to determine whether the decreased hypoxic ventilatory response previously identified is due to the mechanical abnormalities of the respiratory system or to low respiratory center output. The mouth pressure produced by inspiratory muscle activity, a reflection of respiratory center output, was measured at 100 msec of inspiration against an occluded airway at functional residual capacity. At end-tidal oxygen tension (PETO2) of 80 mm Hg, inspiratory muscle activity was greater in asthmatic patients than in normal subjects for the same level of ventilation, but at PETO2 of 40 mm Hg, both inspiratory muscle activity and ventilation were lower in asthmatic patients. Consequently, the changes in inspiratory muscle activity and ventilation per mm Hg change in PETO2 were lower in the asthmatic patients. To generate the same ventilation during progressive hypoxia, more inspiratory muscle activity was needed by asthmatic patients. We concluded that the decreased hypoxic ventilation in asthmatic patients resulted from both decreased respiratory center output and from mechanical abnormalities of the respiratory system.", "contents": "Ventilation response and drive during hypoxia in adult patients with asthma. We studied ventilation and inspiratory muscle activity during progressive isocapnic hypoxia in adult asthmatic patients to determine whether the decreased hypoxic ventilatory response previously identified is due to the mechanical abnormalities of the respiratory system or to low respiratory center output. The mouth pressure produced by inspiratory muscle activity, a reflection of respiratory center output, was measured at 100 msec of inspiration against an occluded airway at functional residual capacity. At end-tidal oxygen tension (PETO2) of 80 mm Hg, inspiratory muscle activity was greater in asthmatic patients than in normal subjects for the same level of ventilation, but at PETO2 of 40 mm Hg, both inspiratory muscle activity and ventilation were lower in asthmatic patients. Consequently, the changes in inspiratory muscle activity and ventilation per mm Hg change in PETO2 were lower in the asthmatic patients. To generate the same ventilation during progressive hypoxia, more inspiratory muscle activity was needed by asthmatic patients. We concluded that the decreased hypoxic ventilation in asthmatic patients resulted from both decreased respiratory center output and from mechanical abnormalities of the respiratory system."}
{"id": "PMID:467115", "title": "Falsely high refractometric readings for the specific gravity of pleural fluid.", "content": "For 128 pleural fluids, the relationship between the protein content and the reading for the specific gravity obtained from a refractometer calibrated for urinary specific gravity was analyzed. The refractometer gave falsely high levels for the specific gravity of the pleural fluid. A reading for 1.019 (rather than 1.016) corresponded to a concentration of protein of 3.0 gm/100 ml, and each deviation of 0.005 (rather than 0.003) coresponded to a concentration of 1 gm/100 ml; however, determinations of the concentration of protein in the pleural fluid directly from the refractometer's scale for protein (calibrated for serum) was rapid and accurate. Calculation of the protein content of the pleural fluid from the reading for specific gravity on the refractometer is erroneous and sometimes misleading.", "contents": "Falsely high refractometric readings for the specific gravity of pleural fluid. For 128 pleural fluids, the relationship between the protein content and the reading for the specific gravity obtained from a refractometer calibrated for urinary specific gravity was analyzed. The refractometer gave falsely high levels for the specific gravity of the pleural fluid. A reading for 1.019 (rather than 1.016) corresponded to a concentration of protein of 3.0 gm/100 ml, and each deviation of 0.005 (rather than 0.003) coresponded to a concentration of 1 gm/100 ml; however, determinations of the concentration of protein in the pleural fluid directly from the refractometer's scale for protein (calibrated for serum) was rapid and accurate. Calculation of the protein content of the pleural fluid from the reading for specific gravity on the refractometer is erroneous and sometimes misleading."}
{"id": "PMID:467116", "title": "Electrophysiologic properties of methyldopa in man.", "content": "There is little information on the effects of methyldopa on the human conduction system. His bundle ECGs were obtained in 11 patients before and after the intravenous infusion of 100 mg of methyldopa. Antegrade refractory periods were obtained with the extrastimulus method. The significant results were as follows: the sinus rate was 71 +/- 4 beats per minute before, and 65 +/- 3 beats per minute after methyldopa (P less than 0.01). The mean A-H interval at a paced rate of 120 beats per minute was 113 +/- 14 msec before, and 135 +/- 18 msec after, methyldopa (P less than 0.05). The mean atrioventricular nodal functional refractory period was 430 +/- 23 msec before and 452 +/- 24 msec after methyldopa administration (P less than 0.001). The mean effective refractory period was 385 +/- 29 msec before, and 388 +/- 27 msec after methyldopa (P less than 0.01). The sinus node recovery time in the control state was 989 +/- 55 msec and 1102 +/- 66 msec after methyldopa infusion (P less than 0.05). Thus, methyldopa can impair conduction through the atrioventricular node and depress the sinus node.", "contents": "Electrophysiologic properties of methyldopa in man. There is little information on the effects of methyldopa on the human conduction system. His bundle ECGs were obtained in 11 patients before and after the intravenous infusion of 100 mg of methyldopa. Antegrade refractory periods were obtained with the extrastimulus method. The significant results were as follows: the sinus rate was 71 +/- 4 beats per minute before, and 65 +/- 3 beats per minute after methyldopa (P less than 0.01). The mean A-H interval at a paced rate of 120 beats per minute was 113 +/- 14 msec before, and 135 +/- 18 msec after, methyldopa (P less than 0.05). The mean atrioventricular nodal functional refractory period was 430 +/- 23 msec before and 452 +/- 24 msec after methyldopa administration (P less than 0.001). The mean effective refractory period was 385 +/- 29 msec before, and 388 +/- 27 msec after methyldopa (P less than 0.01). The sinus node recovery time in the control state was 989 +/- 55 msec and 1102 +/- 66 msec after methyldopa infusion (P less than 0.05). Thus, methyldopa can impair conduction through the atrioventricular node and depress the sinus node."}
{"id": "PMID:467117", "title": "Unusual combination of pulmonary sequestration and funnel chest.", "content": "Cases of pulmonary sequestration and cases of funnel chest are frequently accompanied by other anomalies, although the combination of pulmonary sequestration and funnel chest is rare. In this study, we report ten cases of pulmonary sequestration, nine of which had the combination of pulmonary sequestration and other anomalies; four evidenced the unusual combination of pulmonary sequestration and funnel chest.", "contents": "Unusual combination of pulmonary sequestration and funnel chest. Cases of pulmonary sequestration and cases of funnel chest are frequently accompanied by other anomalies, although the combination of pulmonary sequestration and funnel chest is rare. In this study, we report ten cases of pulmonary sequestration, nine of which had the combination of pulmonary sequestration and other anomalies; four evidenced the unusual combination of pulmonary sequestration and funnel chest."}
{"id": "PMID:467119", "title": "Aneurysm of the left sinus of Valsalva draining into the right atrium.", "content": "We report the case of an arteriovenous fistula connecting the aorta to the right atrium, due to a ruptured aneurysm of the left sinus of Valsalva. Clinical, echocardiographic, and angiographic aspects of this unusual lesion are discussed and correlated. Emphasis is put on the diagnostic value of the echocardiogram; a characteristic dense echo parallel to the posterior aortic wall is seen, along with signs of right ventricular volume overload. Combined with the case history and auscultatory finding (a continuous murmur on the right sternal edge), this may lead to the correct diagnosis by noninvasive means.", "contents": "Aneurysm of the left sinus of Valsalva draining into the right atrium. We report the case of an arteriovenous fistula connecting the aorta to the right atrium, due to a ruptured aneurysm of the left sinus of Valsalva. Clinical, echocardiographic, and angiographic aspects of this unusual lesion are discussed and correlated. Emphasis is put on the diagnostic value of the echocardiogram; a characteristic dense echo parallel to the posterior aortic wall is seen, along with signs of right ventricular volume overload. Combined with the case history and auscultatory finding (a continuous murmur on the right sternal edge), this may lead to the correct diagnosis by noninvasive means."}
{"id": "PMID:467120", "title": "Gastric rupture complicating post-extubation laryngeal edema and the use of a manual resuscitation bag.", "content": "This report describes a patient who developed gastric rupture as a result of post-extubation laryngeal edema and the use of a manual resuscitation bag. The association between oxygen administraton and gastric rupture is reviewed.", "contents": "Gastric rupture complicating post-extubation laryngeal edema and the use of a manual resuscitation bag. This report describes a patient who developed gastric rupture as a result of post-extubation laryngeal edema and the use of a manual resuscitation bag. The association between oxygen administraton and gastric rupture is reviewed."}
{"id": "PMID:467121", "title": "Recurrent miliary tuberculosis secondary to infected ventriculoatrial shunt.", "content": "The placing of a ventriculoatrial anastomosis because of elevated CSF pressure secondary to tuberculous meningitis resulted in repeated dissemination of Mycobacterium tuberculosis from the anastomosis. The consequent clinical picture showed recurrent appearance and clearing of miliary tuberculosis of the lung in spite of antituberculosis chemotherapy. While this possibility was considered early, the diagnosis was not established until the shunt was replaced shortly before the patient's death.", "contents": "Recurrent miliary tuberculosis secondary to infected ventriculoatrial shunt. The placing of a ventriculoatrial anastomosis because of elevated CSF pressure secondary to tuberculous meningitis resulted in repeated dissemination of Mycobacterium tuberculosis from the anastomosis. The consequent clinical picture showed recurrent appearance and clearing of miliary tuberculosis of the lung in spite of antituberculosis chemotherapy. While this possibility was considered early, the diagnosis was not established until the shunt was replaced shortly before the patient's death."}
{"id": "PMID:467122", "title": "Prostaglandin E1 infusion in the hypoplastic left heart syndrome.", "content": "Aortic infusion of prostaglandin E1 in a moribund infant resulted in marked clinical improvement. Appropriate studies were conducted and the diagnosis of hypoplastic left heart syndrome was made. Although the outcome was unaltered, the improved clinical condition allowed us to exclude potentially curable defects such as aortic coarctation and interruption.", "contents": "Prostaglandin E1 infusion in the hypoplastic left heart syndrome. Aortic infusion of prostaglandin E1 in a moribund infant resulted in marked clinical improvement. Appropriate studies were conducted and the diagnosis of hypoplastic left heart syndrome was made. Although the outcome was unaltered, the improved clinical condition allowed us to exclude potentially curable defects such as aortic coarctation and interruption."}
{"id": "PMID:467123", "title": "Echocardiographic features of mitral obstruction due to bacterial endocarditis.", "content": "We describe a patient with mitral valvular obstruction due to vegetative endocarditis. The diagnosis was made before surgery by M-mode and two-dimensional echo-cardiograms, which revealed a mass of echoes obstructing the mitral orifice. This was confirmed subsequently at surgery. Both modes of echocardiography are of value in the noninvasive diagnosis of mitral valvular obstruction due to vegetative endocarditis, a condition which may be amenable to surgery for valvular replacement.", "contents": "Echocardiographic features of mitral obstruction due to bacterial endocarditis. We describe a patient with mitral valvular obstruction due to vegetative endocarditis. The diagnosis was made before surgery by M-mode and two-dimensional echo-cardiograms, which revealed a mass of echoes obstructing the mitral orifice. This was confirmed subsequently at surgery. Both modes of echocardiography are of value in the noninvasive diagnosis of mitral valvular obstruction due to vegetative endocarditis, a condition which may be amenable to surgery for valvular replacement."}
{"id": "PMID:467128", "title": "Corrective socialization in foster care of children.", "content": "While the phrase, \"interminable foster care\" is becoming a damning clich\u00e9 in the dialogues of juvenile courts and child welfare organizations, many children are growing up in foster homes. Human love must move us to approve and enrich these foster families; childhood is too brief and important to be knowingly allowed disapproved or impoverished experience. This paper describes the foster situation: families, children, and the involved professionals. It then presents a teachable developmental theory weaved from threads of numerous known theories, and describes a process whereby \"interminable\" foster experience was used therapeutically for a group of handicapped homeless children.", "contents": "Corrective socialization in foster care of children. While the phrase, \"interminable foster care\" is becoming a damning clich\u00e9 in the dialogues of juvenile courts and child welfare organizations, many children are growing up in foster homes. Human love must move us to approve and enrich these foster families; childhood is too brief and important to be knowingly allowed disapproved or impoverished experience. This paper describes the foster situation: families, children, and the involved professionals. It then presents a teachable developmental theory weaved from threads of numerous known theories, and describes a process whereby \"interminable\" foster experience was used therapeutically for a group of handicapped homeless children."}
{"id": "PMID:467129", "title": "The awesome burden upon the child who must keep a family secret.", "content": "This paper describes a series of young patients hospitalized in a psychiatric facility because they presented symptoms indicative of a psychotic disorder when, in fact, the youngsters were dealing with the strain of keeping a family secret hidden. Case presentation oriented to how the burden prohibited proper psychological development and the manner of how these secrets came to light are offered. The commonality of patients exhibiting this phenomenon is discussed.", "contents": "The awesome burden upon the child who must keep a family secret. This paper describes a series of young patients hospitalized in a psychiatric facility because they presented symptoms indicative of a psychotic disorder when, in fact, the youngsters were dealing with the strain of keeping a family secret hidden. Case presentation oriented to how the burden prohibited proper psychological development and the manner of how these secrets came to light are offered. The commonality of patients exhibiting this phenomenon is discussed."}
{"id": "PMID:467130", "title": "Games parents play with normal and high-risk infants.", "content": "Game playing during early infant-parent interactions provides a context for learning conversational turntaking and contingent responsivity. The types and frequency of \"universally\" recognized infant games were observed during face-to-face interactions of normal and high risk infant-parent dyads when the infants were four months old. Approximately six different games were played for approximately one-third of the interaction time. Except during interactions with normal male infants mothers and fathers played games equally as often. The high-risk infant-parent dyads engaged in game playing less frequently than the normal infant-parent dyads. These differences are discussed in the context of the high-risk infants being \"difficult\" babies and relatively less responsive.", "contents": "Games parents play with normal and high-risk infants. Game playing during early infant-parent interactions provides a context for learning conversational turntaking and contingent responsivity. The types and frequency of \"universally\" recognized infant games were observed during face-to-face interactions of normal and high risk infant-parent dyads when the infants were four months old. Approximately six different games were played for approximately one-third of the interaction time. Except during interactions with normal male infants mothers and fathers played games equally as often. The high-risk infant-parent dyads engaged in game playing less frequently than the normal infant-parent dyads. These differences are discussed in the context of the high-risk infants being \"difficult\" babies and relatively less responsive."}
{"id": "PMID:467131", "title": "A retrospective analysis of the TATs of children at risk who subsequently broke down.", "content": "A retrospective evaluation of stories told to three Thematic Apperception Test (TAT) cards by children at risk isolated six characteristics that were associated with functioning six to 10 years later. The characteristics included lack of individual initiative, denial of mother-child relationships, denial of negative outcomes, and lack of autonomy. The TATs of children at risk (due to one parent's hospitalization for a psychotic illness) who subsequently suffered major decompensation themselves (requiring hospitalization or intensive outpatient therapy) could be discriminated from those of children at risk with apparently normal development. The TAT characteristics were independent of the level of adjustment at the time the TATs were administered, not related to the child's IQ, socioeconomic status, race, or other family characteristics, and moderately negatively correlated with story length.", "contents": "A retrospective analysis of the TATs of children at risk who subsequently broke down. A retrospective evaluation of stories told to three Thematic Apperception Test (TAT) cards by children at risk isolated six characteristics that were associated with functioning six to 10 years later. The characteristics included lack of individual initiative, denial of mother-child relationships, denial of negative outcomes, and lack of autonomy. The TATs of children at risk (due to one parent's hospitalization for a psychotic illness) who subsequently suffered major decompensation themselves (requiring hospitalization or intensive outpatient therapy) could be discriminated from those of children at risk with apparently normal development. The TAT characteristics were independent of the level of adjustment at the time the TATs were administered, not related to the child's IQ, socioeconomic status, race, or other family characteristics, and moderately negatively correlated with story length."}
{"id": "PMID:467132", "title": "Consulting to children in crisis.", "content": "Although community consultation is common for psychiatrists, such activity is usually carried out on an elective rather than emergency basis. In a world troubled by community disaster situations--children are often at risk. Psychiatrists, through the use of skillful crisis consultation, can be of great help to these young people. This report describes the effort of a mental health consultation team to meet the needs of a large population of children under acute stress.", "contents": "Consulting to children in crisis. Although community consultation is common for psychiatrists, such activity is usually carried out on an elective rather than emergency basis. In a world troubled by community disaster situations--children are often at risk. Psychiatrists, through the use of skillful crisis consultation, can be of great help to these young people. This report describes the effort of a mental health consultation team to meet the needs of a large population of children under acute stress."}
{"id": "PMID:467167", "title": "Conservation of repeated DNA sequences in aneuploid human tumor cells.", "content": "A series of human neuroectodermal tumors, all containing more than the normal diploid DNA, and each with its own distinct chromosome mode, were studied using restriction enzyme cleavage and specific DNA sequence hybridization. Methods described were quite sensitive and quantitative and as few as 40 molecules with a given restriction site were reproducibly detected in total nuclear DNA. Analysis of several fluorescent gel bands associated with different chromosomal domains revealed no changes between any of the tumor and normal cells. Specific probe hybridization, using purified complex repeating sequences, indicated fidelity of base sequence, as well as preservation of the relative amounts of each of a number of minor related multimers in both the tumor and normal cells. Centromeric regions containing arrays of such sequences may be maintained in these tumor cells and furthermore it is possible that some of these cells are polyploid with respect to DNA sequences, rather than aneuploid as their chromosome profiles suggest.", "contents": "Conservation of repeated DNA sequences in aneuploid human tumor cells. A series of human neuroectodermal tumors, all containing more than the normal diploid DNA, and each with its own distinct chromosome mode, were studied using restriction enzyme cleavage and specific DNA sequence hybridization. Methods described were quite sensitive and quantitative and as few as 40 molecules with a given restriction site were reproducibly detected in total nuclear DNA. Analysis of several fluorescent gel bands associated with different chromosomal domains revealed no changes between any of the tumor and normal cells. Specific probe hybridization, using purified complex repeating sequences, indicated fidelity of base sequence, as well as preservation of the relative amounts of each of a number of minor related multimers in both the tumor and normal cells. Centromeric regions containing arrays of such sequences may be maintained in these tumor cells and furthermore it is possible that some of these cells are polyploid with respect to DNA sequences, rather than aneuploid as their chromosome profiles suggest."}
{"id": "PMID:467168", "title": "The nucleolus organizers of Plethodon and Aneides located by in situ nucleic acid hybridization with Xenopus 3H-ribosomal RNA.", "content": "The main clusters of ribosomal genes, or nucleolus organizers, have been located by in situ nucleic acid hybridization of Xenopus laevis 3H labelled ribosomal RNA to mitotic chromosomes in squash preparations of intestinal epithelium from 7 species of Plethodon and 3 species of Aneides. The species used were chosen on account of having well known karyotypes and genome sizes. The Plethodon species covered a range of genome size of 20--69.4 pg. The locations of those nucleolus organizers that could be detected by autoradiography after in situ hybridization varied from species to species, and in Aneides there were differences between two populations of the same species. On the other hand, some distantly related species of Plethodon, with widely different genome sizes, had nucleolus organizers at corresponding positions. The results are discussed in relation to ideas on karyotype stability, homosequentiality and chromosome repatterning.", "contents": "The nucleolus organizers of Plethodon and Aneides located by in situ nucleic acid hybridization with Xenopus 3H-ribosomal RNA. The main clusters of ribosomal genes, or nucleolus organizers, have been located by in situ nucleic acid hybridization of Xenopus laevis 3H labelled ribosomal RNA to mitotic chromosomes in squash preparations of intestinal epithelium from 7 species of Plethodon and 3 species of Aneides. The species used were chosen on account of having well known karyotypes and genome sizes. The Plethodon species covered a range of genome size of 20--69.4 pg. The locations of those nucleolus organizers that could be detected by autoradiography after in situ hybridization varied from species to species, and in Aneides there were differences between two populations of the same species. On the other hand, some distantly related species of Plethodon, with widely different genome sizes, had nucleolus organizers at corresponding positions. The results are discussed in relation to ideas on karyotype stability, homosequentiality and chromosome repatterning."}
{"id": "PMID:467169", "title": "The activity of two heat shock loci of Drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complementary DNA.", "content": "Complementary DNA was made to poly A+ nuclear or polysomal RNA isolated from heat shock tissue culture cells of Drosophila hydei. A number of loci other than the four major heat shock loci are labelled after in situ hybridization of these cDNA preparations, while solution hybridization indicated that only about 10% of the cDNA was specific for heat shocked cells. Removal of the fraction of cDNA which could react with 25 degrees C RNA and subsequent in situ hybridization of heat shock specific cDNA indicated that locus 4-81 B, a major salivary gland heat shock locus, is also active at 25 degrees C in tissue culture cells, while locus 4-85 B is specifically activated by heat shock in tissue culture cells. This latter locus is not seen to be clearly puffed in salivary glands, but was shown to be active in that tissue both by direct autoradiography of salivary gland chromosomes after 3H-uridine labeling and by hybridization of cDNA to chromosomal RNA.", "contents": "The activity of two heat shock loci of Drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complementary DNA. Complementary DNA was made to poly A+ nuclear or polysomal RNA isolated from heat shock tissue culture cells of Drosophila hydei. A number of loci other than the four major heat shock loci are labelled after in situ hybridization of these cDNA preparations, while solution hybridization indicated that only about 10% of the cDNA was specific for heat shocked cells. Removal of the fraction of cDNA which could react with 25 degrees C RNA and subsequent in situ hybridization of heat shock specific cDNA indicated that locus 4-81 B, a major salivary gland heat shock locus, is also active at 25 degrees C in tissue culture cells, while locus 4-85 B is specifically activated by heat shock in tissue culture cells. This latter locus is not seen to be clearly puffed in salivary glands, but was shown to be active in that tissue both by direct autoradiography of salivary gland chromosomes after 3H-uridine labeling and by hybridization of cDNA to chromosomal RNA."}
{"id": "PMID:467171", "title": "Circadian patterns of growth hormone and cortisol secretions in narcoleptic patients.", "content": "Narcolepsy, a disorder which is clinically characterized by recurring episodes of sleep during the day, frequently associated with nocturnal disrupted sleep and polygraphically by sleep onset REM periods, has been studied in regard to the relationships between GH, cortisol secretion and sleep. Ten narcoleptics were polygraphically recorded during 24 h after one night's adaptation. Blood samples were taken every 20 min for GH and cortisol immuno-assays. Three narcoleptics were recorded twice 2 months later and 2 normal subjects served as controls. The following results were obtained: GH secretory pattern was different in narcoleptics and controls; two groups were identified: the first one showed a very low basal GH secretion with rare and small secretory peaks not clearly linked with sleep. One subject of this group recorded twice showed a similar pattern. The second group exhibited a higher basal secretion with many peaks without any well-defined relationship to sleep stages. The pattern was again consistent in two recordings of 2 subjects in this group. Furthermore GH secretion rose significantly (p less than 0.01) between 2100 and 0000 with no apparent relationship to sleep stages. The cortisol secretory pattern showed a significant rise (p less than 0.01) between 0400 and 1000 and was not different in narcoleptics and controls. In narcolepsy the monophasic sleep-wakefulness cycle is disrupted. The GH secretion pattern is modified whereas the circadian pattern of cortisol secretion is normal and independent of the sleep-wakefulness cycle of the narcoleptic patient.", "contents": "Circadian patterns of growth hormone and cortisol secretions in narcoleptic patients. Narcolepsy, a disorder which is clinically characterized by recurring episodes of sleep during the day, frequently associated with nocturnal disrupted sleep and polygraphically by sleep onset REM periods, has been studied in regard to the relationships between GH, cortisol secretion and sleep. Ten narcoleptics were polygraphically recorded during 24 h after one night's adaptation. Blood samples were taken every 20 min for GH and cortisol immuno-assays. Three narcoleptics were recorded twice 2 months later and 2 normal subjects served as controls. The following results were obtained: GH secretory pattern was different in narcoleptics and controls; two groups were identified: the first one showed a very low basal GH secretion with rare and small secretory peaks not clearly linked with sleep. One subject of this group recorded twice showed a similar pattern. The second group exhibited a higher basal secretion with many peaks without any well-defined relationship to sleep stages. The pattern was again consistent in two recordings of 2 subjects in this group. Furthermore GH secretion rose significantly (p less than 0.01) between 2100 and 0000 with no apparent relationship to sleep stages. The cortisol secretory pattern showed a significant rise (p less than 0.01) between 0400 and 1000 and was not different in narcoleptics and controls. In narcolepsy the monophasic sleep-wakefulness cycle is disrupted. The GH secretion pattern is modified whereas the circadian pattern of cortisol secretion is normal and independent of the sleep-wakefulness cycle of the narcoleptic patient."}
{"id": "PMID:467170", "title": "Circadian changes in adrenal dopamine-beta-hydroxylase activity: dependency of change at darkness onset, and the effect of pinealectomy, on animal strain and age.", "content": "In a previous study we found a daily darkness- and pineal-dependent rise in adrenal dopamine-beta-hydroxylase (DBH) activity in 4-month-old S1 rats. The present investigation shows that aged (12 to 14-month-old) S1 rats do not have such a change in DBH activity. Similarly in the relatively younger (100-day-old) rats of a different (Holtzman) strain such a change was not found. Also pinealectomy did not seem to have any effect on adrenal DBH in either of these two strains of rats, young or old at times just before and after the onset of darkness. In both S1 and Holtzman rats, the concentration of copper-sensitive endogenous inhibitors of DBH remained unaltered following the surgical extirpation of pineal. Photoperiodic change from light to dark also had no significant effect on the inhibitor content in these two strains of rats. It is concluded that there are age and strain dependencies in the behavior of adrenal DBH activity at the daily onset of darkness.", "contents": "Circadian changes in adrenal dopamine-beta-hydroxylase activity: dependency of change at darkness onset, and the effect of pinealectomy, on animal strain and age. In a previous study we found a daily darkness- and pineal-dependent rise in adrenal dopamine-beta-hydroxylase (DBH) activity in 4-month-old S1 rats. The present investigation shows that aged (12 to 14-month-old) S1 rats do not have such a change in DBH activity. Similarly in the relatively younger (100-day-old) rats of a different (Holtzman) strain such a change was not found. Also pinealectomy did not seem to have any effect on adrenal DBH in either of these two strains of rats, young or old at times just before and after the onset of darkness. In both S1 and Holtzman rats, the concentration of copper-sensitive endogenous inhibitors of DBH remained unaltered following the surgical extirpation of pineal. Photoperiodic change from light to dark also had no significant effect on the inhibitor content in these two strains of rats. It is concluded that there are age and strain dependencies in the behavior of adrenal DBH activity at the daily onset of darkness."}
{"id": "PMID:467172", "title": "Oscillatory nature of DNA, RNA, protein and lipid metabolisms in antigen-stimulated lymphocytes.", "content": "The primary immune response in inbred strain C3H/He to SRBC, or BSA is induced. The DNA, RNA, lipid precursor uptakes and total proteins are studied for 14 successive days following immunization, in splenic lymphocytes. Significant variations are noticed in the course of immune response of stimulated lymphocytes concerning their DNA, RNA, lipid and protein metabolisms respectively. The results reveal that there are several wave-metabolisms. The kinetic studies further indicate that course of different metabolisms would be of oscillatory nature. The activity peaks of synthesis may vary according to different antigens. Oscillatory systems would be 'excited' by antigenic stimulation. These results and hypotheses are discussed here.", "contents": "Oscillatory nature of DNA, RNA, protein and lipid metabolisms in antigen-stimulated lymphocytes. The primary immune response in inbred strain C3H/He to SRBC, or BSA is induced. The DNA, RNA, lipid precursor uptakes and total proteins are studied for 14 successive days following immunization, in splenic lymphocytes. Significant variations are noticed in the course of immune response of stimulated lymphocytes concerning their DNA, RNA, lipid and protein metabolisms respectively. The results reveal that there are several wave-metabolisms. The kinetic studies further indicate that course of different metabolisms would be of oscillatory nature. The activity peaks of synthesis may vary according to different antigens. Oscillatory systems would be 'excited' by antigenic stimulation. These results and hypotheses are discussed here."}
{"id": "PMID:467178", "title": "Treatment of pneumatosis cystoides intestinalis with high FIO2: report of two cases.", "content": "We have successfully treated two patients with symptomatic pneumatosis cystoides intestinalis using a high FIO2 delivered by face mask, with administration interrupted at meal times. While we advocate oxygen therapy for this disease, caution must be taken to avoid the complications of high PaO2, namely proliferative and fibrotic lung changes. Close attention should be paid to the development of increasing cough, dyspnea and shortness of breath. As the frequency of the diagnosis of pneumatosis cystoides intestinalis increases, the success of high inspired oxygen as therapy should be recognized.", "contents": "Treatment of pneumatosis cystoides intestinalis with high FIO2: report of two cases. We have successfully treated two patients with symptomatic pneumatosis cystoides intestinalis using a high FIO2 delivered by face mask, with administration interrupted at meal times. While we advocate oxygen therapy for this disease, caution must be taken to avoid the complications of high PaO2, namely proliferative and fibrotic lung changes. Close attention should be paid to the development of increasing cough, dyspnea and shortness of breath. As the frequency of the diagnosis of pneumatosis cystoides intestinalis increases, the success of high inspired oxygen as therapy should be recognized."}
{"id": "PMID:467177", "title": "Squamous-cell carcinoma of the proximal colon: report of a case and review of the literature.", "content": "A case of an undoubted squamous-cell carcinoma arising at the hepatic flexure in the colon of a middle-aged man is reported. Review of the literature and exclusion of cases not strictly comparable suggest that pure squamous-cell carcinoma of the colon is rare, this being only the fifteenth such tumor reported, and only the second recorded case of such a tumor of the hepatic flexure. Possible pathogenesis is suggested.", "contents": "Squamous-cell carcinoma of the proximal colon: report of a case and review of the literature. A case of an undoubted squamous-cell carcinoma arising at the hepatic flexure in the colon of a middle-aged man is reported. Review of the literature and exclusion of cases not strictly comparable suggest that pure squamous-cell carcinoma of the colon is rare, this being only the fifteenth such tumor reported, and only the second recorded case of such a tumor of the hepatic flexure. Possible pathogenesis is suggested."}
{"id": "PMID:467180", "title": "Rectal carcinoma with solitary cerebral metastasis: report of a case and review of the literature.", "content": "A 54-year-old man underwent abdominoperineal resection for carcinoma of the rectum. Three months later, neurologic signs and symptoms developed. A brain scan, as well as angiographic studies, demonstrated a space-occupying lesion in the right parietal area. A solitary tumor was removed at craniotomy. Histologic examination revealed a metastatic adenocarcinoma with a rectal primary tumor. For two years the patient remained well, but then signs of local perineal recurrence developed. Treatment with cobalt irradiation and chemotherapy was unsuccessful. Autopsy revealed local recurrence with numerous distant metastases; however, examination of the brain failed to show a recurrent metastatic focus.", "contents": "Rectal carcinoma with solitary cerebral metastasis: report of a case and review of the literature. A 54-year-old man underwent abdominoperineal resection for carcinoma of the rectum. Three months later, neurologic signs and symptoms developed. A brain scan, as well as angiographic studies, demonstrated a space-occupying lesion in the right parietal area. A solitary tumor was removed at craniotomy. Histologic examination revealed a metastatic adenocarcinoma with a rectal primary tumor. For two years the patient remained well, but then signs of local perineal recurrence developed. Treatment with cobalt irradiation and chemotherapy was unsuccessful. Autopsy revealed local recurrence with numerous distant metastases; however, examination of the brain failed to show a recurrent metastatic focus."}
{"id": "PMID:467183", "title": "An unusual malformation of the vermiform appendix simulating endometriosis: a case report with discussion of differential diagnosis.", "content": "A 48-year-old nulliparous woman with a long history of pelvic endometriosis underwent a gynecologic operation, and a swollen and indurated appendix was removed because of suspected endometriosis. Pathologic examination revealed no endometriosis, but examination of the distal appendix showed structural disorganization of its entire wall, with lack of proper differentiation of its normal coats and irregular overgrowth of fibroadipose, fibromuscular, and neural elements. Differential diagnoses, including endometriosis, carcinoid tumor and neurofibromatosis, are discussed.", "contents": "An unusual malformation of the vermiform appendix simulating endometriosis: a case report with discussion of differential diagnosis. A 48-year-old nulliparous woman with a long history of pelvic endometriosis underwent a gynecologic operation, and a swollen and indurated appendix was removed because of suspected endometriosis. Pathologic examination revealed no endometriosis, but examination of the distal appendix showed structural disorganization of its entire wall, with lack of proper differentiation of its normal coats and irregular overgrowth of fibroadipose, fibromuscular, and neural elements. Differential diagnoses, including endometriosis, carcinoid tumor and neurofibromatosis, are discussed."}
{"id": "PMID:467184", "title": "Mucocele of the appendix: diagnosis and surgical management.", "content": "Cystadenomas of the appendix are an unusual clinical entity. Despite a recognized constellation of findings, the diagnosis is not usually made prior to surgical exploration. A 65-year-old man is reported in whom preoperative evaluation, including sonography and angiography, resulted in the correct diagnosis confirmed at surgery. The appropriate use of newer diagnostic modalities will establish the preoperative diagnosis and facilitate surgical intervention. Appendectomy is curative for this condition, as neither regional nor distant lymph node metastases or hematogenous spread have been reported. When symptoms due to pseudomyxoma peritoneai occur, a continued aggressive surgical approach is justified.", "contents": "Mucocele of the appendix: diagnosis and surgical management. Cystadenomas of the appendix are an unusual clinical entity. Despite a recognized constellation of findings, the diagnosis is not usually made prior to surgical exploration. A 65-year-old man is reported in whom preoperative evaluation, including sonography and angiography, resulted in the correct diagnosis confirmed at surgery. The appropriate use of newer diagnostic modalities will establish the preoperative diagnosis and facilitate surgical intervention. Appendectomy is curative for this condition, as neither regional nor distant lymph node metastases or hematogenous spread have been reported. When symptoms due to pseudomyxoma peritoneai occur, a continued aggressive surgical approach is justified."}
{"id": "PMID:467186", "title": "Prevention of intussusception in Peutz-Jeghers syndrome.", "content": "Peutz-Jeghers syndrome is a familial disease characterized by mucocutaneous pigmentation and intestinal polyposis. Small-intestinal intussusception is a common complication. Preservation otion technique to prevent intussusception is described.", "contents": "Prevention of intussusception in Peutz-Jeghers syndrome. Peutz-Jeghers syndrome is a familial disease characterized by mucocutaneous pigmentation and intestinal polyposis. Small-intestinal intussusception is a common complication. Preservation otion technique to prevent intussusception is described."}
{"id": "PMID:467192", "title": "Guidelines for use of flexible fiberoptic colonoscopy in management of patients with colorectal neoplasia.", "content": "Flexible fiberoptic colonoscopy, to have the maximum effect upon the management of patients having, suspected of, or at risk of having colorectal neoplastic disease, must be applied in accordance with substantial guidelines. Schemes for the application of colonoscopy have been designed for the early and accurate diagnosis of benign and malignant polypoid lesions. Colonoscopy has proven effective in confirming radiographic findings and the removal of pre-malignant and superficially malignant lesions. When applied in accordance with appropriate guidelines, flexible fiberoptic colonoscopy should provide not only secure surveillance in those individuals at high risk, but also should reduce appreciably the morbidity and mortality of colorectal neoplasia.", "contents": "Guidelines for use of flexible fiberoptic colonoscopy in management of patients with colorectal neoplasia. Flexible fiberoptic colonoscopy, to have the maximum effect upon the management of patients having, suspected of, or at risk of having colorectal neoplastic disease, must be applied in accordance with substantial guidelines. Schemes for the application of colonoscopy have been designed for the early and accurate diagnosis of benign and malignant polypoid lesions. Colonoscopy has proven effective in confirming radiographic findings and the removal of pre-malignant and superficially malignant lesions. When applied in accordance with appropriate guidelines, flexible fiberoptic colonoscopy should provide not only secure surveillance in those individuals at high risk, but also should reduce appreciably the morbidity and mortality of colorectal neoplasia."}
{"id": "PMID:467193", "title": "Surgery plus freezing as a technique for treating pilonidal disease.", "content": "Pilonidal disease in 12 patients was treated by a technique of incision and cryosurgery. All patients had complete healing and no recurrence after the 12 months of follow up. The advantage of abbreviated hospitalization is obvious and the early return to activities of daily living greatly appreciated by the patient. This procedure is recommended as a means of thoroughly treating disease with little morbidity.", "contents": "Surgery plus freezing as a technique for treating pilonidal disease. Pilonidal disease in 12 patients was treated by a technique of incision and cryosurgery. All patients had complete healing and no recurrence after the 12 months of follow up. The advantage of abbreviated hospitalization is obvious and the early return to activities of daily living greatly appreciated by the patient. This procedure is recommended as a means of thoroughly treating disease with little morbidity."}
{"id": "PMID:467194", "title": "A randomized controlled trial to compare anal dilatation with lateral subcutaneous sphincterotomy for anal fissure.", "content": "A prospective randomized trial has compared manual dilatation of the anus (MDA)during general anesthesia with lateral subcutaneous spincterotomy (LSS) during local anesthesia for the management of anal fissure in 156 patients. The two groups were similar with respect to age, sex and symptoms. There was no difference in the duration of time off work or early complications of treatment but, four months after operation, 93 per cent claimed to have been improved by MDA compared with 78 per cent after LSS (P less than 0.05). Recurrent fissure was recorded in 13 patients after LSS (29 per cent) compared with four (10 per cent) after MDA (P less than 0.02). There was a significant reduction in anal pressure at four months (P less than 0.02) after MDA, (123 +/- 31 to 97 +/-33) and LSS (127 +/- 36 to 104 +/- 32), but the anal pressure remained unchanged by operation in all patients where pressures were measured with recurrent fissure. These data indicate that MDA gives better results than LSS for treatment of anal fissure and that successful treatment is associated with a reduction in anal pressure.", "contents": "A randomized controlled trial to compare anal dilatation with lateral subcutaneous sphincterotomy for anal fissure. A prospective randomized trial has compared manual dilatation of the anus (MDA)during general anesthesia with lateral subcutaneous spincterotomy (LSS) during local anesthesia for the management of anal fissure in 156 patients. The two groups were similar with respect to age, sex and symptoms. There was no difference in the duration of time off work or early complications of treatment but, four months after operation, 93 per cent claimed to have been improved by MDA compared with 78 per cent after LSS (P less than 0.05). Recurrent fissure was recorded in 13 patients after LSS (29 per cent) compared with four (10 per cent) after MDA (P less than 0.02). There was a significant reduction in anal pressure at four months (P less than 0.02) after MDA, (123 +/- 31 to 97 +/-33) and LSS (127 +/- 36 to 104 +/- 32), but the anal pressure remained unchanged by operation in all patients where pressures were measured with recurrent fissure. These data indicate that MDA gives better results than LSS for treatment of anal fissure and that successful treatment is associated with a reduction in anal pressure."}
{"id": "PMID:467195", "title": "The morbidity and mortality of colostomy closure.", "content": "Restrospective analysis was done of 304 patients who underwent colostomy closure at Henry Ford Hospital between 1967 and 1977. A mortality of less than 0.3 per cent and a morbidity rate of 14 per cent, with an average hospital stay of 15 days, is hereby reported. Wound infection was the most common complication with an incidence of 9.5 per cent. Late complications during the study period were less than 3 per cent. In our experience, if and intraperitoneal closure technique with resectiona and anastomosis is used, colostomy closure can be a safe procedure with minimal mortality and morbidity. We believe colostomy closure should be considered as nothing less than a major colonic resection.", "contents": "The morbidity and mortality of colostomy closure. Restrospective analysis was done of 304 patients who underwent colostomy closure at Henry Ford Hospital between 1967 and 1977. A mortality of less than 0.3 per cent and a morbidity rate of 14 per cent, with an average hospital stay of 15 days, is hereby reported. Wound infection was the most common complication with an incidence of 9.5 per cent. Late complications during the study period were less than 3 per cent. In our experience, if and intraperitoneal closure technique with resectiona and anastomosis is used, colostomy closure can be a safe procedure with minimal mortality and morbidity. We believe colostomy closure should be considered as nothing less than a major colonic resection."}
{"id": "PMID:467196", "title": "The outpatient management of acute hemorrhoidal disease.", "content": "A procedure for the outpatient management of acute hemorrhoidal disease is presented. Treatment, using local anesthesia with hyaluronidase, is directed to (1) relieve pain, (2) decrease edema, (3) treat appropriately the internal hemorrhoidal disease, and (4) treat the external thrombosis if present. Adequate treatment of the acute disease will, in a significant proportion of patients, avoid further surgical intervention. Eperience with results of this modality of treatment in patients is presented.", "contents": "The outpatient management of acute hemorrhoidal disease. A procedure for the outpatient management of acute hemorrhoidal disease is presented. Treatment, using local anesthesia with hyaluronidase, is directed to (1) relieve pain, (2) decrease edema, (3) treat appropriately the internal hemorrhoidal disease, and (4) treat the external thrombosis if present. Adequate treatment of the acute disease will, in a significant proportion of patients, avoid further surgical intervention. Eperience with results of this modality of treatment in patients is presented."}
{"id": "PMID:467197", "title": "Duodenocolic fistula: an unusual complication of duodenal diverticulum.", "content": "The management of two patients with benign duodenocolic fistula is reported. In both patients, laparotomy clearly demonstrated the origin of a fistulous tract arising from a duodenal diverticulum. Microscopic examination of the diverticula failed to disclose the presence of either gastric or pancreatic tissue.", "contents": "Duodenocolic fistula: an unusual complication of duodenal diverticulum. The management of two patients with benign duodenocolic fistula is reported. In both patients, laparotomy clearly demonstrated the origin of a fistulous tract arising from a duodenal diverticulum. Microscopic examination of the diverticula failed to disclose the presence of either gastric or pancreatic tissue."}
{"id": "PMID:467198", "title": "An intra-abdominal foreign body presenting as a colonic tumor: review of literature and report of a case.", "content": "A case is reported in which a patient seemed to show the clinical picture of a colonic tumor, but was found to have a retained laparotomy pad from an exploratory operation which had been performed nine years earlier. This case of an intra-abdominal foreign body demonstrated many interesting and instructive clinical, radiologic, and surgical features with approaches to the diagnosis and management of this condition.", "contents": "An intra-abdominal foreign body presenting as a colonic tumor: review of literature and report of a case. A case is reported in which a patient seemed to show the clinical picture of a colonic tumor, but was found to have a retained laparotomy pad from an exploratory operation which had been performed nine years earlier. This case of an intra-abdominal foreign body demonstrated many interesting and instructive clinical, radiologic, and surgical features with approaches to the diagnosis and management of this condition."}
{"id": "PMID:467201", "title": "Rectal anatomy following Ripstein's operation for prolapse studied by cineradiography.", "content": "Eleven patients operated on for rectal prolapse, according to the method described by Ripstein, were examined pre- and postoperatively with cineradiography of the rectum. The patients were asked to fill in a questionnaire to evaluate their histories of constipation. There is no apparent anatomic explanation for postoperative constipation. One patient had a rectal stricture and another, a recurrence in the shape of an intussusception.", "contents": "Rectal anatomy following Ripstein's operation for prolapse studied by cineradiography. Eleven patients operated on for rectal prolapse, according to the method described by Ripstein, were examined pre- and postoperatively with cineradiography of the rectum. The patients were asked to fill in a questionnaire to evaluate their histories of constipation. There is no apparent anatomic explanation for postoperative constipation. One patient had a rectal stricture and another, a recurrence in the shape of an intussusception."}
{"id": "PMID:467202", "title": "A new concept of the anatomy of the anal sphincter mechanism and the physiology of defecation. VI. The central abscess: a new clinicopathologic entity in the genesis of anorectal suppuration.", "content": "The central abscess is a new clinicopathologic entity which plays an important role in the pathogenesis of anorectal abscess and fistula. Eight cases are presented. The abscess occupies the central space, which lies between the longitudinal muscle and the base loop of the external sphincter. The anatomic pattern and the clinical manifestations of the abscess are discussed. The abscess extended, in some cases, to the subcutaneous space and perianal skin, to the anal canal proper, or to the intersphincteric spaces. This is due to the fact that the central space communicates with all of the other perianal spaces along the central septa. A new theory in the pathogenesis of anorectal suppuration and fistula is put forward. It is believed that all anorectal abscesses or fistulas originate as central space infection which spreads to the other perianal spaces. Proper diagnosis and early drainage of the central abscess prevent further spread of suppuration to other spaces.", "contents": "A new concept of the anatomy of the anal sphincter mechanism and the physiology of defecation. VI. The central abscess: a new clinicopathologic entity in the genesis of anorectal suppuration. The central abscess is a new clinicopathologic entity which plays an important role in the pathogenesis of anorectal abscess and fistula. Eight cases are presented. The abscess occupies the central space, which lies between the longitudinal muscle and the base loop of the external sphincter. The anatomic pattern and the clinical manifestations of the abscess are discussed. The abscess extended, in some cases, to the subcutaneous space and perianal skin, to the anal canal proper, or to the intersphincteric spaces. This is due to the fact that the central space communicates with all of the other perianal spaces along the central septa. A new theory in the pathogenesis of anorectal suppuration and fistula is put forward. It is believed that all anorectal abscesses or fistulas originate as central space infection which spreads to the other perianal spaces. Proper diagnosis and early drainage of the central abscess prevent further spread of suppuration to other spaces."}
{"id": "PMID:467208", "title": "Does motilin regulate canine interdigestive gastric motility?", "content": "Our objective was to determine whether motilin increases the frequency of cyclic interdigestive motor activity in the canine proximal stomach. In 4 conscious dogs with autotransplanted proximal gastric pouches and gastrointestinal electrodes, intravenous infusions of motilin (0.6 microgram/kg body wt/hr) increased the frequency of the interdigestive cycles by 30% both in the pouch and in the main gastrointestinal tract. However, the 3-min interval between the end of a cycle in the pouch and the end of a cycle in the duodenum was unchanged by motilin. In control experiments, without motilin infusion, the concentration of endogenous motilin in the plasma during the intense contractile phase of the cycles (overall mean 385 pg/ml) was greater in each dog (P less than 0.01) than the concentration during the quiescent phase (overall mean, 256 pg/ml). The concentration of plasma motilin was also greater in the contractile phase (mean, 717 pg/ml) than in the quiescent phase (mean, 587 pg/ml) during exogenous infusions of motilin in two of three dogs (P less than 0.01). We concluded that motilin increased the frequency of cyclic interdigestive motor activity in the canine proximal stomach and that the increase was not dependent on intact proximal gastric extrinsic innervation.", "contents": "Does motilin regulate canine interdigestive gastric motility? Our objective was to determine whether motilin increases the frequency of cyclic interdigestive motor activity in the canine proximal stomach. In 4 conscious dogs with autotransplanted proximal gastric pouches and gastrointestinal electrodes, intravenous infusions of motilin (0.6 microgram/kg body wt/hr) increased the frequency of the interdigestive cycles by 30% both in the pouch and in the main gastrointestinal tract. However, the 3-min interval between the end of a cycle in the pouch and the end of a cycle in the duodenum was unchanged by motilin. In control experiments, without motilin infusion, the concentration of endogenous motilin in the plasma during the intense contractile phase of the cycles (overall mean 385 pg/ml) was greater in each dog (P less than 0.01) than the concentration during the quiescent phase (overall mean, 256 pg/ml). The concentration of plasma motilin was also greater in the contractile phase (mean, 717 pg/ml) than in the quiescent phase (mean, 587 pg/ml) during exogenous infusions of motilin in two of three dogs (P less than 0.01). We concluded that motilin increased the frequency of cyclic interdigestive motor activity in the canine proximal stomach and that the increase was not dependent on intact proximal gastric extrinsic innervation."}
{"id": "PMID:467203", "title": "Anal sphincteric pressure studies in complete rectal prolapse.", "content": "Anal sphincteric pressure studies of patients with complete rectal prolapse demonstrated that: 1) The resting yield pressure of the internal anal sphincter in patients with complete rectal prolapse was not significantly different from that of control subjects. Thus, it seems to have no role in the etiology of prolapse. 2) The maximal sphincteric pressure of the external anal sphincter was significantly less in patients with prolapse than in controls. This suggested weakness in the effective functional power of the external anal sphincter in complete prolapse. 3) Anal continence could be achieved by the ability to maintain full contraction of the external anal sphincter for more than 40 seconds, which was the minimum for control subjects. Incontinent patients could not do this.", "contents": "Anal sphincteric pressure studies in complete rectal prolapse. Anal sphincteric pressure studies of patients with complete rectal prolapse demonstrated that: 1) The resting yield pressure of the internal anal sphincter in patients with complete rectal prolapse was not significantly different from that of control subjects. Thus, it seems to have no role in the etiology of prolapse. 2) The maximal sphincteric pressure of the external anal sphincter was significantly less in patients with prolapse than in controls. This suggested weakness in the effective functional power of the external anal sphincter in complete prolapse. 3) Anal continence could be achieved by the ability to maintain full contraction of the external anal sphincter for more than 40 seconds, which was the minimum for control subjects. Incontinent patients could not do this."}
{"id": "PMID:467204", "title": "Intestinal gas in ulcerative colitis.", "content": "Intestinal gas was measured planimetrically on plain abdominal films from 25 healthy subjects and 47 patients with active ulcerative colitis (mild, moderate, severe and toxic megacolon). Compared with controls, significant colonic distention was found in toxic megacolon. Gas in the small bowel was significantly increased in toxic megacolon and in severe colitis but was within the normal range in the mild and moderate forms. Two groups of patients with severe colitis were identified, showing either normal or increased gas content in the small bowel. Two of seven patients in the latter group developed toxic megacolon in spite of intensive medical treatment. Intestinal gas values showed a linear correlation with arterial blood pH, ESR and body temperature. No correlation was found between intestinal gas and other features of severity. It is suggested that an abnormal collection of gas in the small bowel loops associated with metabolic alkalosis corresponds to an early stage of a toxic complication (\"impending megacolon\").", "contents": "Intestinal gas in ulcerative colitis. Intestinal gas was measured planimetrically on plain abdominal films from 25 healthy subjects and 47 patients with active ulcerative colitis (mild, moderate, severe and toxic megacolon). Compared with controls, significant colonic distention was found in toxic megacolon. Gas in the small bowel was significantly increased in toxic megacolon and in severe colitis but was within the normal range in the mild and moderate forms. Two groups of patients with severe colitis were identified, showing either normal or increased gas content in the small bowel. Two of seven patients in the latter group developed toxic megacolon in spite of intensive medical treatment. Intestinal gas values showed a linear correlation with arterial blood pH, ESR and body temperature. No correlation was found between intestinal gas and other features of severity. It is suggested that an abnormal collection of gas in the small bowel loops associated with metabolic alkalosis corresponds to an early stage of a toxic complication (\"impending megacolon\")."}
{"id": "PMID:467205", "title": "Idiopathic ulcerative colitis in Istanbul: clinical review of 204 cases.", "content": "A clinical review of 204 cases of idiopathic ulcerative colitis (IUC) seen in an insurance hospital in Istanbul during a period of nine years is presented. The criteria of diagnosis were based on history and results of rectosigmoidoscopic, biopsy, and barium-enema examinations. In the majority of cases (74 per cent), the disease manifested in the mild form, despite a history of one to five years' duration at the time of diagnosis. These patients usually had single attacks, with recovery in reponse to treatment. The mortality rate for the whole series was 3.9 per cent. Death occurred in eight cases of patients who had the severe form of the disease involving the entire colon, with fulminating courses. One additional death was due to an accident. Treatment consisted of a high-protein, low residue diet, vitamins, rest, sulfa drugs and steroids. Azathioprine, in addition, was used in fulminating cases. Surgery supplemented medical therapy in 19 cases. Indications for surgical treatment were resistance to medical management, cancer, and polyposis. Only two patients (1.0 per cent) were found to have cancer during the follow-up period. These two were operated upon. Idiopathic ulcerative colitis is not a rare entity; with awareness and use of appropriate diagnosis facilities, more cases are being discovered.", "contents": "Idiopathic ulcerative colitis in Istanbul: clinical review of 204 cases. A clinical review of 204 cases of idiopathic ulcerative colitis (IUC) seen in an insurance hospital in Istanbul during a period of nine years is presented. The criteria of diagnosis were based on history and results of rectosigmoidoscopic, biopsy, and barium-enema examinations. In the majority of cases (74 per cent), the disease manifested in the mild form, despite a history of one to five years' duration at the time of diagnosis. These patients usually had single attacks, with recovery in reponse to treatment. The mortality rate for the whole series was 3.9 per cent. Death occurred in eight cases of patients who had the severe form of the disease involving the entire colon, with fulminating courses. One additional death was due to an accident. Treatment consisted of a high-protein, low residue diet, vitamins, rest, sulfa drugs and steroids. Azathioprine, in addition, was used in fulminating cases. Surgery supplemented medical therapy in 19 cases. Indications for surgical treatment were resistance to medical management, cancer, and polyposis. Only two patients (1.0 per cent) were found to have cancer during the follow-up period. These two were operated upon. Idiopathic ulcerative colitis is not a rare entity; with awareness and use of appropriate diagnosis facilities, more cases are being discovered."}
{"id": "PMID:467210", "title": "Insulin and glucagon levels in liver cirrhosis. Relationship with plasma amino acid imbalance of chronic hepatic encephalopathy.", "content": "Alterations in insulin and glucagon levels might account for the plasma amino acid imbalance of cirrhotics. In order to verify this hypothesis we evaluated basal insulin, glucagon, branched-chain amino acids, aromatic amino acids, and free tryptophan in 13 controls and 37 cirrhotics divided on the basis of their mental state; in 4 patients the hormonal and amino acid patterns were sequentially studied during various stages of encephalopathy. Glucagon is high in cirrhotics and progressively increases with the worsening of the mental state. Free tryptophan and aromatic amino acids show a similar behavior and significantly correlate with glucagon levels (r = 0.67 and r = 0.81, respectively). On the other hand insulin levels, which are high in cirrhotics without encephalopathy, fall in the presence of deep coma. Insulin did not correlate with any of the plasma amino acids considered. Our data suggest that the catabolic state associated with increased glucagon levels may account for some of the alterations in the plasma amino acid profiles of cirrhotics. Portal-systemic shunting does not seem to be the common cause of both hyperglucagonemia and hyperaminoacidemia. Decreased branched-chain amino acid levels may be related to factors different from those involved in the alterations of carbohydrate homeostasis.", "contents": "Insulin and glucagon levels in liver cirrhosis. Relationship with plasma amino acid imbalance of chronic hepatic encephalopathy. Alterations in insulin and glucagon levels might account for the plasma amino acid imbalance of cirrhotics. In order to verify this hypothesis we evaluated basal insulin, glucagon, branched-chain amino acids, aromatic amino acids, and free tryptophan in 13 controls and 37 cirrhotics divided on the basis of their mental state; in 4 patients the hormonal and amino acid patterns were sequentially studied during various stages of encephalopathy. Glucagon is high in cirrhotics and progressively increases with the worsening of the mental state. Free tryptophan and aromatic amino acids show a similar behavior and significantly correlate with glucagon levels (r = 0.67 and r = 0.81, respectively). On the other hand insulin levels, which are high in cirrhotics without encephalopathy, fall in the presence of deep coma. Insulin did not correlate with any of the plasma amino acids considered. Our data suggest that the catabolic state associated with increased glucagon levels may account for some of the alterations in the plasma amino acid profiles of cirrhotics. Portal-systemic shunting does not seem to be the common cause of both hyperglucagonemia and hyperaminoacidemia. Decreased branched-chain amino acid levels may be related to factors different from those involved in the alterations of carbohydrate homeostasis."}
{"id": "PMID:467206", "title": "Comparative study of a standardized senna liquid and castor oil in preparing patients for radiographic examination of the colon.", "content": "X-Prep Liquid, used as a radiologic bowel evacuant, was found to be more effective, better tolerated, and more readily accepted than castor oil. The efficacy of a single full dose of this peristaltic stimulant was confirmed, and a divided dose of X-Prep Liquid was practically as effective and produced less griping and cramping. This less intensive divided-dose approach appears to be appropriate, especially in aged or debilitated patients.", "contents": "Comparative study of a standardized senna liquid and castor oil in preparing patients for radiographic examination of the colon. X-Prep Liquid, used as a radiologic bowel evacuant, was found to be more effective, better tolerated, and more readily accepted than castor oil. The efficacy of a single full dose of this peristaltic stimulant was confirmed, and a divided dose of X-Prep Liquid was practically as effective and produced less griping and cramping. This less intensive divided-dose approach appears to be appropriate, especially in aged or debilitated patients."}
{"id": "PMID:467211", "title": "Inhibition of rat basal pancreatic secretion by intraduodenal bile.", "content": "The effect on basal exocrine pancreatic secretion of diversion from and reintroduction into the duodenum of bile has been studied on conscious rats provided with pancreatic, biliary and duodenal fistulae. Diversion of bile from the intestine augmented protein output by 30%. After an eight-hour diversion recirculation of bile into the duodenum reduced pancreatic protein output by 30%; volume being not significantly modified. When either bile was diverted or the main bile duct was ligated, a similar inhibition of protein secretion was observed after intraduodenal injections of 20 mM solutions of taurocholate, taurochenodeoxycholate, chenodeoxycholate, and cholate, and of synthetic mixed micelles (bile salts, lecithin). Inhibitory action of bile salts on pancreatic secretion was seen equally whether or not the bile salts were in free or conjugated form or pancreatic juice returned to the intestine. We conclude that unlike man and the dog, bile as well as pancreatic juice inhibits the basal pancreatic exocrine secretion of the rat.", "contents": "Inhibition of rat basal pancreatic secretion by intraduodenal bile. The effect on basal exocrine pancreatic secretion of diversion from and reintroduction into the duodenum of bile has been studied on conscious rats provided with pancreatic, biliary and duodenal fistulae. Diversion of bile from the intestine augmented protein output by 30%. After an eight-hour diversion recirculation of bile into the duodenum reduced pancreatic protein output by 30%; volume being not significantly modified. When either bile was diverted or the main bile duct was ligated, a similar inhibition of protein secretion was observed after intraduodenal injections of 20 mM solutions of taurocholate, taurochenodeoxycholate, chenodeoxycholate, and cholate, and of synthetic mixed micelles (bile salts, lecithin). Inhibitory action of bile salts on pancreatic secretion was seen equally whether or not the bile salts were in free or conjugated form or pancreatic juice returned to the intestine. We conclude that unlike man and the dog, bile as well as pancreatic juice inhibits the basal pancreatic exocrine secretion of the rat."}
{"id": "PMID:467213", "title": "A clinical scoring system for chronic inflammatory bowel disease in children.", "content": "A clinical scoring system for the assessment of children with chronic inflammatory bowel disease has been devised. A close correlation is demonstrated between severity of disease and the level of serum albumin. The clinical score is simple to perform, sensitive to changes in clinical status, reproducible by different observers, and specifically designed to evaluate inflammatory bowel disease in children and adolescents. The clinical score is a useful adjunct in the management of children with chronic inflammatory bowel disease and can be used in prospective studies of various therapeutic modalities.", "contents": "A clinical scoring system for chronic inflammatory bowel disease in children. A clinical scoring system for the assessment of children with chronic inflammatory bowel disease has been devised. A close correlation is demonstrated between severity of disease and the level of serum albumin. The clinical score is simple to perform, sensitive to changes in clinical status, reproducible by different observers, and specifically designed to evaluate inflammatory bowel disease in children and adolescents. The clinical score is a useful adjunct in the management of children with chronic inflammatory bowel disease and can be used in prospective studies of various therapeutic modalities."}
{"id": "PMID:467214", "title": "Malignant histiocytosis of the intestine.", "content": "Four cases of malignant histiocytosis of the intestine (MHI) are described in detail, with emphasis on the clinical features and laboratory findings that may lead to a diagnosis. MHI is a rapidly progressive, usually fatal condition, which may occur in a setting of adult celiac disease or apparently spontaneously; all patients, however, have villous atrophy and crypt hyperplasia of the jejunum. Characteristic symptoms are malaise, weight loss, abdominal pain, and diarrhea followed, after a period of weeks or months, by acute small intestinal obstruction, perforation, or bleeding. Because of the high mortality associated with MHI, suggestive symptomatology and investigations should, in the absence of a definitive diagnosis, lead to an urgent laparotomy with a view to chemotherapy if the diagnosis is made.", "contents": "Malignant histiocytosis of the intestine. Four cases of malignant histiocytosis of the intestine (MHI) are described in detail, with emphasis on the clinical features and laboratory findings that may lead to a diagnosis. MHI is a rapidly progressive, usually fatal condition, which may occur in a setting of adult celiac disease or apparently spontaneously; all patients, however, have villous atrophy and crypt hyperplasia of the jejunum. Characteristic symptoms are malaise, weight loss, abdominal pain, and diarrhea followed, after a period of weeks or months, by acute small intestinal obstruction, perforation, or bleeding. Because of the high mortality associated with MHI, suggestive symptomatology and investigations should, in the absence of a definitive diagnosis, lead to an urgent laparotomy with a view to chemotherapy if the diagnosis is made."}
{"id": "PMID:467215", "title": "Esophageal motility in progressive systemic sclerosis (PSS). Comparison of cine-radiographic and manometric evaluation.", "content": "The evaluation of structural and functional abnormalities of the esophagus by manometry (using perfused tubes) and cine-radiography were compared in 25 subjects with progressive systemic sclerosis (PSS). Motility by both procedures was definitely abnormal in 19 subjects and normal in 3. The remaining 3 subjects had abnormal motility on manometry, coupled with cine-esophagrams interpreted as normal. Many structural abnormalities not demonstrated by manometry were identified by cine-radiography. Therefore, the initial diagnostic examination of motility in PSS should be the cine-esophagram.", "contents": "Esophageal motility in progressive systemic sclerosis (PSS). Comparison of cine-radiographic and manometric evaluation. The evaluation of structural and functional abnormalities of the esophagus by manometry (using perfused tubes) and cine-radiography were compared in 25 subjects with progressive systemic sclerosis (PSS). Motility by both procedures was definitely abnormal in 19 subjects and normal in 3. The remaining 3 subjects had abnormal motility on manometry, coupled with cine-esophagrams interpreted as normal. Many structural abnormalities not demonstrated by manometry were identified by cine-radiography. Therefore, the initial diagnostic examination of motility in PSS should be the cine-esophagram."}
{"id": "PMID:467218", "title": "Follow-up of a flatulent patient.", "content": "This paper describes a low-flatulence diet developed by an extremely flatulent patient. Based on meticulous recording of each passage of flatus, the patient employed an elimination diet to determine what foods were responsible for his gas production. The diet reduced the frequency of his gas passage from 34 +/- 7 to 17 +/- 5 times per day (normal: 14 +/- 6) and similar reductions were observed by two other flatulent patients during adherence to the diet. The rectal gas of each of these subjects largely consisted of two gases (CO2 and H2) which result from bacterial fermentation of carbohydrates. The diet, which is low in lactose and wheat products, presumably minimizes the quantity of carbohydrates delivered to the colonic bacteria.", "contents": "Follow-up of a flatulent patient. This paper describes a low-flatulence diet developed by an extremely flatulent patient. Based on meticulous recording of each passage of flatus, the patient employed an elimination diet to determine what foods were responsible for his gas production. The diet reduced the frequency of his gas passage from 34 +/- 7 to 17 +/- 5 times per day (normal: 14 +/- 6) and similar reductions were observed by two other flatulent patients during adherence to the diet. The rectal gas of each of these subjects largely consisted of two gases (CO2 and H2) which result from bacterial fermentation of carbohydrates. The diet, which is low in lactose and wheat products, presumably minimizes the quantity of carbohydrates delivered to the colonic bacteria."}
{"id": "PMID:467246", "title": "[Diuretic resistant ascites in hepatic cirrhosis and renal insufficiency (author's transl)].", "content": "In decompensated hepatic cirrhosis the glomerular filtration rate is reduced. Use of diuretics frequently leads to hyponatraemia, hypotension, hypovolaemia and oliguria. The ensuing renal insufficiency is reversible when the peritoneal fluid is redirected into the vascular system. For this purpose a subcutaneously implantable pump developed by Agishi was used in a 37-year-old patient permitting drainage of the ascites from the peritoneal cavity into the superior vena cava using an actively operated pumping mechanism. Use of the pump in the patient abolished the ascites, considerably improved renal function, equilibrated electrolyte metabolism and improved renal response to diuretics.", "contents": "[Diuretic resistant ascites in hepatic cirrhosis and renal insufficiency (author's transl)]. In decompensated hepatic cirrhosis the glomerular filtration rate is reduced. Use of diuretics frequently leads to hyponatraemia, hypotension, hypovolaemia and oliguria. The ensuing renal insufficiency is reversible when the peritoneal fluid is redirected into the vascular system. For this purpose a subcutaneously implantable pump developed by Agishi was used in a 37-year-old patient permitting drainage of the ascites from the peritoneal cavity into the superior vena cava using an actively operated pumping mechanism. Use of the pump in the patient abolished the ascites, considerably improved renal function, equilibrated electrolyte metabolism and improved renal response to diuretics."}
{"id": "PMID:467253", "title": "[Immunity against poliomyelitis in the German Federal Republic (author's transl)].", "content": "The immunity of the populations of several countries of the GFR against poliovirus 1, 2 and 3 was investigated in 11 laboratories. Sera of 4707 persons aged 0 to 30 years were assessed for neutralising antibodies (serum dilution 1:4) against the 3 types of poliovirus. 34.5% of the 4707 investigated sera had no neutralising antibodies at least against one type of the poliovirus, 4.7% showed no antibodies against all 3 types of the virus. Especially children aged 0 to 4 years were protected incompletely against the 3 types of poliovirus. In comparison with similar investigations of 1969 and 1972 no decisive change of immunity of the population of the GFR against poliomyelitis has occurred.", "contents": "[Immunity against poliomyelitis in the German Federal Republic (author's transl)]. The immunity of the populations of several countries of the GFR against poliovirus 1, 2 and 3 was investigated in 11 laboratories. Sera of 4707 persons aged 0 to 30 years were assessed for neutralising antibodies (serum dilution 1:4) against the 3 types of poliovirus. 34.5% of the 4707 investigated sera had no neutralising antibodies at least against one type of the poliovirus, 4.7% showed no antibodies against all 3 types of the virus. Especially children aged 0 to 4 years were protected incompletely against the 3 types of poliovirus. In comparison with similar investigations of 1969 and 1972 no decisive change of immunity of the population of the GFR against poliomyelitis has occurred."}
{"id": "PMID:467254", "title": "[Serum thyroglobulin estimation for follow-up of patients with thyroid carcinoma (author's transl)].", "content": "Thyroglobulin concentrations were determined radioimmunologically in the sera of 53 patients operated for thyroid carcinoma. 37 patients without metastases or recurrence had either very low (up to maximally 15 ng/ml) or non-demonstrable thyroglobulin concentrations, whereas 16 patients with metastases showed values of more than 40 ng/ml up to maximal values of more than 1000 ng/ml. In the majority of patients follow-up controls, in part up to 2 years, were performed. A far-reaching conformity of thyroglobulin concentrations with scintigraphic or clinical findings of metastases was shown. Thyroglobulin estimation in operated thyroid cancer patients is thus an important contribution to the aftercare.", "contents": "[Serum thyroglobulin estimation for follow-up of patients with thyroid carcinoma (author's transl)]. Thyroglobulin concentrations were determined radioimmunologically in the sera of 53 patients operated for thyroid carcinoma. 37 patients without metastases or recurrence had either very low (up to maximally 15 ng/ml) or non-demonstrable thyroglobulin concentrations, whereas 16 patients with metastases showed values of more than 40 ng/ml up to maximal values of more than 1000 ng/ml. In the majority of patients follow-up controls, in part up to 2 years, were performed. A far-reaching conformity of thyroglobulin concentrations with scintigraphic or clinical findings of metastases was shown. Thyroglobulin estimation in operated thyroid cancer patients is thus an important contribution to the aftercare."}
{"id": "PMID:467255", "title": "[Sorbitol and xylitol in post-operative infusion therapy (author's transl)].", "content": "The effect of a 24-hour continuous infusion of sorbitol in the recommended maximum dosage of 0.25 g . kg-1 . h-1 or of xylitol in the recommended maximum dosage of 0.125 g . kg-1 . h-1 on metabolic parameters was investigated in seven healthy adult men. The results demonstrate the continued applicability for longer than the recommended time as well as the acceptability of continuous infusion of polyols used clinically with combined solutions.", "contents": "[Sorbitol and xylitol in post-operative infusion therapy (author's transl)]. The effect of a 24-hour continuous infusion of sorbitol in the recommended maximum dosage of 0.25 g . kg-1 . h-1 or of xylitol in the recommended maximum dosage of 0.125 g . kg-1 . h-1 on metabolic parameters was investigated in seven healthy adult men. The results demonstrate the continued applicability for longer than the recommended time as well as the acceptability of continuous infusion of polyols used clinically with combined solutions."}
{"id": "PMID:467264", "title": "[Nosocomial infections with Providencia stuartii (Proteus inconstans) (author's transl)].", "content": "48% of all isolates of Providencia stuartii obtained within a period of one year, originated from the urine of catheterised patients, whereas another 37% were found in the respiratory tract of patients with intubation. The incidence of Providenica stuartii correlated with the period during which iatrogenic measures had been applied, as well as with a preceding course with antibiotics.", "contents": "[Nosocomial infections with Providencia stuartii (Proteus inconstans) (author's transl)]. 48% of all isolates of Providencia stuartii obtained within a period of one year, originated from the urine of catheterised patients, whereas another 37% were found in the respiratory tract of patients with intubation. The incidence of Providenica stuartii correlated with the period during which iatrogenic measures had been applied, as well as with a preceding course with antibiotics."}
{"id": "PMID:467265", "title": "[Treatment with clomiphene of endocrine primary and secondary infertility in women (author's transl)].", "content": "Clomiphene (Dyneric) in a daily dosage of 100--300 mg, partially combined with HCG (n = 12), was used in the treatment of 86 women aged 19 to 39 years with primary or secondary infertility of endocrine origin in 296 menstrual cycles. In 67 cases (77.9%) ovulation could be induced and in 38 cases (44.2%) pregnancy occurred. In addition, in 4 cases pregnancy occurred two months after cessation of treatment. Combination therapy with HCG was successful in some of the cases resistant to clomiphene.", "contents": "[Treatment with clomiphene of endocrine primary and secondary infertility in women (author's transl)]. Clomiphene (Dyneric) in a daily dosage of 100--300 mg, partially combined with HCG (n = 12), was used in the treatment of 86 women aged 19 to 39 years with primary or secondary infertility of endocrine origin in 296 menstrual cycles. In 67 cases (77.9%) ovulation could be induced and in 38 cases (44.2%) pregnancy occurred. In addition, in 4 cases pregnancy occurred two months after cessation of treatment. Combination therapy with HCG was successful in some of the cases resistant to clomiphene."}
{"id": "PMID:467275", "title": "[Frequency of life-threatening complications associated with exercise testing (author's transl)].", "content": "Most of the statistics on complications of ergometric exercise tests come from the United States and are largely related to treadmill ergometry. A questionnaire was sent in the summer of 1978 to 198 investigative units in the German-speaking regions. The results of 1065 923 person-tests were made available. Exercise testing of 353 638 sports-persons revealed no serious complications. On the other hand, testing of 712 285 patients, predominantly with coronary heart disease, lead to 17 deaths and a total of 96 life-threatening complications. The result of this survey indicates that one must expect one such complication for every 7500 ergometry tests. The danger of pulmonary oedema on exercise in recumbency is about five times higher than that on sitting or standing. The most frequent complication was ventricular fibrillation. A defibrillator should therefore always be immediately available during exercise tests.", "contents": "[Frequency of life-threatening complications associated with exercise testing (author's transl)]. Most of the statistics on complications of ergometric exercise tests come from the United States and are largely related to treadmill ergometry. A questionnaire was sent in the summer of 1978 to 198 investigative units in the German-speaking regions. The results of 1065 923 person-tests were made available. Exercise testing of 353 638 sports-persons revealed no serious complications. On the other hand, testing of 712 285 patients, predominantly with coronary heart disease, lead to 17 deaths and a total of 96 life-threatening complications. The result of this survey indicates that one must expect one such complication for every 7500 ergometry tests. The danger of pulmonary oedema on exercise in recumbency is about five times higher than that on sitting or standing. The most frequent complication was ventricular fibrillation. A defibrillator should therefore always be immediately available during exercise tests."}
{"id": "PMID:467276", "title": "[Localised aspergillosis and oxalosis of the lung caused by aspergillus niger: soil of ornamental plants as a reservoir of aspergilli (case report) (author's transl)].", "content": "Haemoptysis occurred in a 64-year-old man with a history of pulmonary tuberculosis, negative for M. tuberculosis. Precipitating antibodies to Aspergillus niger were found in serum and the fungus found in sputum. Autopsy revealed aspergilloma, colonization of the pulmonary cavity by A. niger and local oxalosis if the cavity wall. Ornamental plant soil containing A. niger conidia, as well as the air in the patient's living-room, was the probable source of infection.", "contents": "[Localised aspergillosis and oxalosis of the lung caused by aspergillus niger: soil of ornamental plants as a reservoir of aspergilli (case report) (author's transl)]. Haemoptysis occurred in a 64-year-old man with a history of pulmonary tuberculosis, negative for M. tuberculosis. Precipitating antibodies to Aspergillus niger were found in serum and the fungus found in sputum. Autopsy revealed aspergilloma, colonization of the pulmonary cavity by A. niger and local oxalosis if the cavity wall. Ornamental plant soil containing A. niger conidia, as well as the air in the patient's living-room, was the probable source of infection."}
{"id": "PMID:467303", "title": "[Scientific work in the training of health personnel].", "content": "This document makes a series of statements about the limitations to scientific and technological development in Latin America and about recent trends in efforts to overcome them. The writer reviews the situation regarding health research in the Region and lists the respective shortcomings: \"it is meager and disjointed, does not spring from the health problems of Latin American countries, and--like the training of health manpower, and of physicians in particular--is seen as entirely divorced from social reality, responding to outside influences that are sometimes entirely extraneous to the countries' own needs.\" She emphasizes that, while many health manpower training centers are striving to make their instruction more relevant to real life in their countries, no attempt is made to develop a scientific understanding of the health problems to which the educational process must be geared. She analyzes the role of research in student training and regards it as important to keep in mind that both research and instruction must aim for the same goal: development of science and technology in the service of the community. In closing, she asserts that the Latin American university must be committed to science in the service of society, which means that it must pass tha knowledge on to the population and join with it in the solution of its problems.", "contents": "[Scientific work in the training of health personnel]. This document makes a series of statements about the limitations to scientific and technological development in Latin America and about recent trends in efforts to overcome them. The writer reviews the situation regarding health research in the Region and lists the respective shortcomings: \"it is meager and disjointed, does not spring from the health problems of Latin American countries, and--like the training of health manpower, and of physicians in particular--is seen as entirely divorced from social reality, responding to outside influences that are sometimes entirely extraneous to the countries' own needs.\" She emphasizes that, while many health manpower training centers are striving to make their instruction more relevant to real life in their countries, no attempt is made to develop a scientific understanding of the health problems to which the educational process must be geared. She analyzes the role of research in student training and regards it as important to keep in mind that both research and instruction must aim for the same goal: development of science and technology in the service of the community. In closing, she asserts that the Latin American university must be committed to science in the service of society, which means that it must pass tha knowledge on to the population and join with it in the solution of its problems."}
{"id": "PMID:467304", "title": "[Social medicine and medical education].", "content": "The author reviews the establishment of social security in Brazil from its origins in 1923 to the establishment of the National Institute of Medical Care and Social Welfare in 1977. He highlights the salient developments in the history of medical education in his country and analyzes the contributions of the welfare system to the training of the physician, particularly through student training in hospitals. The author is of the view that the introduction of social medicine into medical education, including the articulation of social welfare with the university hospitals, would be most salutary for the programs of the national health system.", "contents": "[Social medicine and medical education]. The author reviews the establishment of social security in Brazil from its origins in 1923 to the establishment of the National Institute of Medical Care and Social Welfare in 1977. He highlights the salient developments in the history of medical education in his country and analyzes the contributions of the welfare system to the training of the physician, particularly through student training in hospitals. The author is of the view that the introduction of social medicine into medical education, including the articulation of social welfare with the university hospitals, would be most salutary for the programs of the national health system."}
{"id": "PMID:467305", "title": "[The community health technician and alternative in the training of health manpower].", "content": "This paper describes the general conditions of the social and economic dynamics of the rural area, with emphasis on the development of the factors that affect health in Mexico, and briefly analyzes the system for the education and training of manpower for health care. The author presents arguments in support of an alternative proposal for the training of the \"community health technician\" as a professional produced by four years of study following graduation from secondary school, and trained to take measures that contribute to the integral development of the community by acting upon the factors involved in the occurrence of pathological states, and to be the first contact for persons with medical problems. The course of training is described in terms of its ultimate purpose, which is improvement of the health status of a rural community, and of the general objectives in the \"community development\" and \"medical care\" areas. The course is administered in 25 modules as follows: four introductory modules to acquaint the student with the requirements for dealing with the areas, 11 modules on community development, eight modules on first-contact medical care, and the last two for practical exercises and community work, in which the student is required to bring all his knowledge to bear on a given community. Lastly, the course content of each semester is described together with the distribution of the theoretical and practical work in each module, and the outlook for its success, which points to the possibility of incorporating this resource into programs for extending coverage to rural areas.", "contents": "[The community health technician and alternative in the training of health manpower]. This paper describes the general conditions of the social and economic dynamics of the rural area, with emphasis on the development of the factors that affect health in Mexico, and briefly analyzes the system for the education and training of manpower for health care. The author presents arguments in support of an alternative proposal for the training of the \"community health technician\" as a professional produced by four years of study following graduation from secondary school, and trained to take measures that contribute to the integral development of the community by acting upon the factors involved in the occurrence of pathological states, and to be the first contact for persons with medical problems. The course of training is described in terms of its ultimate purpose, which is improvement of the health status of a rural community, and of the general objectives in the \"community development\" and \"medical care\" areas. The course is administered in 25 modules as follows: four introductory modules to acquaint the student with the requirements for dealing with the areas, 11 modules on community development, eight modules on first-contact medical care, and the last two for practical exercises and community work, in which the student is required to bring all his knowledge to bear on a given community. Lastly, the course content of each semester is described together with the distribution of the theoretical and practical work in each module, and the outlook for its success, which points to the possibility of incorporating this resource into programs for extending coverage to rural areas."}
{"id": "PMID:467306", "title": "[Minimum requirements for the establishment of medical schools. Division of human resources and investigation. PAHO/WHO].", "content": "This paper was presented as a reference document for the Working Group on the subject that met at Salvador, Bahia, Brazil, in December 1977, for the purpose of analyzing the recommendations presented in the report on Basic Principles for the Development of Medical Education in Latin America and the Caribbean Area, and to consider the proposed alternative ways of implementing them. It provides an introduction to each of the main topics that were to be considered by the Working Group, namely: socioeconomic aspects, health practices and the training of medical manpower; the inter-relations between the educational process and health services; institutional and program characteristics of institutions that produce physicians. It also refers to the efforts that have been going forward in the last few years in some physician-training centers, in the form of innovative projects for the teaching of medicine, including early participation of the student in the health practice and his incorporation into the various levels of care; the development of processes for the integration of teaching and service, and, in some cases, of teaching, service, and research; interdisciplinary coordination; the striking of a balance between the biological and social sciences; and further emphasis on study of the social sciences in their applications to the health-illness process.", "contents": "[Minimum requirements for the establishment of medical schools. Division of human resources and investigation. PAHO/WHO]. This paper was presented as a reference document for the Working Group on the subject that met at Salvador, Bahia, Brazil, in December 1977, for the purpose of analyzing the recommendations presented in the report on Basic Principles for the Development of Medical Education in Latin America and the Caribbean Area, and to consider the proposed alternative ways of implementing them. It provides an introduction to each of the main topics that were to be considered by the Working Group, namely: socioeconomic aspects, health practices and the training of medical manpower; the inter-relations between the educational process and health services; institutional and program characteristics of institutions that produce physicians. It also refers to the efforts that have been going forward in the last few years in some physician-training centers, in the form of innovative projects for the teaching of medicine, including early participation of the student in the health practice and his incorporation into the various levels of care; the development of processes for the integration of teaching and service, and, in some cases, of teaching, service, and research; interdisciplinary coordination; the striking of a balance between the biological and social sciences; and further emphasis on study of the social sciences in their applications to the health-illness process."}
{"id": "PMID:467308", "title": "[Effect of halothane (narcothane) on intrauterine fetal development].", "content": "The authors examined the effect of chalotane (narcotann ne-Spofa) on the development of a rat fetus. The rats were divided into groups and were narcotised for a period of 4 hours as well as 4.5 days. One of the group was narcotised from the 8 to the 13 day of gestation. Narcosis was made in chambers with dynamic inhalatory regimen at concentration of narcotane of 10 mg/m3 and 250 mg/m3. There was blastopathogenic effect of narcotane, manifested by an early resorption of nidaded ova, by nonspecific action on the fetus, which inhibited growth and development of the fetus and raised the mortality of the fetuses. The amount of estriol was diminished in mothers, treated with 250 mg/m3 of the drug.", "contents": "[Effect of halothane (narcothane) on intrauterine fetal development]. The authors examined the effect of chalotane (narcotann ne-Spofa) on the development of a rat fetus. The rats were divided into groups and were narcotised for a period of 4 hours as well as 4.5 days. One of the group was narcotised from the 8 to the 13 day of gestation. Narcosis was made in chambers with dynamic inhalatory regimen at concentration of narcotane of 10 mg/m3 and 250 mg/m3. There was blastopathogenic effect of narcotane, manifested by an early resorption of nidaded ova, by nonspecific action on the fetus, which inhibited growth and development of the fetus and raised the mortality of the fetuses. The amount of estriol was diminished in mothers, treated with 250 mg/m3 of the drug."}
{"id": "PMID:467309", "title": "[Changes in the perfusion of the internal organs of rabbits with experimental atherosclerosis treated with a protein hydrolysate].", "content": "The authors examined the vascularization of the internal organs of rabbits with experimental atherosclerosis, induced by the method of Sapirstein with 86-Rubidium. The experiments were carried out on male rabbits of the strain Cincilla, fed with cholesterol in a dose of 0.2 gm/kg of body weight daily for a period of 90 days. Part of the animals were treated with protein hydrolysate in a dose of 5 ml/kg of body weight subcutaneously, but the remaining part of the animals-with saline. There was a reduced vascularization in heart, kidney, intestine, liver, adrenals, pancreas, and other internal organs in rabbits fed with cholesterol and treated with saline. Administration of protein hydrolysate had protective effect on the vascularization of the organs accumulation of 86-Rubidium in a large part of the animals presented values higher than rhose of the control group. The authors suggested that under the influence of amino-acids from the protein hydrolysate the state of the endothelial cells was stabilized and the local, feactions of vascularization were stimulated as well.", "contents": "[Changes in the perfusion of the internal organs of rabbits with experimental atherosclerosis treated with a protein hydrolysate]. The authors examined the vascularization of the internal organs of rabbits with experimental atherosclerosis, induced by the method of Sapirstein with 86-Rubidium. The experiments were carried out on male rabbits of the strain Cincilla, fed with cholesterol in a dose of 0.2 gm/kg of body weight daily for a period of 90 days. Part of the animals were treated with protein hydrolysate in a dose of 5 ml/kg of body weight subcutaneously, but the remaining part of the animals-with saline. There was a reduced vascularization in heart, kidney, intestine, liver, adrenals, pancreas, and other internal organs in rabbits fed with cholesterol and treated with saline. Administration of protein hydrolysate had protective effect on the vascularization of the organs accumulation of 86-Rubidium in a large part of the animals presented values higher than rhose of the control group. The authors suggested that under the influence of amino-acids from the protein hydrolysate the state of the endothelial cells was stabilized and the local, feactions of vascularization were stimulated as well."}
{"id": "PMID:467310", "title": "[Effect of thyrotropic hormone on the iron content and iron-binding capacity in the milk of rabbits].", "content": "The authors administered thyreotropic hormone in a dose of 2 international units/kg of body weight on lactating rabbits intramuscularly. Concentrations of iron and iron-binding capacity in milk were examined at different days (from 7 to 21 days after delivery) of the lactation period of the animals. The obtained results suggested an inhibiting effect of thyreotropin on the content of iron and iron-binding capacity in milk after the first day of treatment. Later there was a gradual recovery of values almost to the initial values of the control day, which indicated transitory and comparatively quickly disappearing hormonal effect. The presumed mechanisms for this effect of thyreotropic hormone were discussed.", "contents": "[Effect of thyrotropic hormone on the iron content and iron-binding capacity in the milk of rabbits]. The authors administered thyreotropic hormone in a dose of 2 international units/kg of body weight on lactating rabbits intramuscularly. Concentrations of iron and iron-binding capacity in milk were examined at different days (from 7 to 21 days after delivery) of the lactation period of the animals. The obtained results suggested an inhibiting effect of thyreotropin on the content of iron and iron-binding capacity in milk after the first day of treatment. Later there was a gradual recovery of values almost to the initial values of the control day, which indicated transitory and comparatively quickly disappearing hormonal effect. The presumed mechanisms for this effect of thyreotropic hormone were discussed."}
{"id": "PMID:467311", "title": "[Effect of laser irradiation on the serological and morphological indices of experimental immune reactivity (a preliminary report)].", "content": "The authors examined guinea pigs, irradiated for a period of six days with helium-neon lazer according to a scheme, proposed by them. They determined the changes in the complement serum activity, blood level of heparine and histamine as well as histologic changes in adrenals, liver and spleen. When they compared the control animals (nonirradiated) with the experimental animals, they established a statisticaly significant elevation of the complement activity in serum, a tendency to lowering of histamine with an increase of heparine and hyperplastic reaction in zona glomerulosa of the adrenals. The obtained results suggested stimulation of immune mechanisms with antiallergic and antinflammatory effect.", "contents": "[Effect of laser irradiation on the serological and morphological indices of experimental immune reactivity (a preliminary report)]. The authors examined guinea pigs, irradiated for a period of six days with helium-neon lazer according to a scheme, proposed by them. They determined the changes in the complement serum activity, blood level of heparine and histamine as well as histologic changes in adrenals, liver and spleen. When they compared the control animals (nonirradiated) with the experimental animals, they established a statisticaly significant elevation of the complement activity in serum, a tendency to lowering of histamine with an increase of heparine and hyperplastic reaction in zona glomerulosa of the adrenals. The obtained results suggested stimulation of immune mechanisms with antiallergic and antinflammatory effect."}
{"id": "PMID:467312", "title": "[Biomechanical studies of rabbit tendons after repeated injections of corticosteroids].", "content": "The purpose of investigation was to determine and compare the mechanical properties of rabbit tendons, injected with celestone as well as noninjected. The authors accepted the dynamic module during cyclic loading and the damaging tension reduced to an unit of body weight of the rabbit. As a result of the experimental biochemical studies they found that tendons, injected with corticosteroids, presented reduced strength and increased deformity in comparison with the noninjected tendons. Histologic examinations, performed additionaly, revealed that the functional properties of the tendons, injected with corticosteroids, were altered before the occurrence of structural changes in the tendinous tissue.", "contents": "[Biomechanical studies of rabbit tendons after repeated injections of corticosteroids]. The purpose of investigation was to determine and compare the mechanical properties of rabbit tendons, injected with celestone as well as noninjected. The authors accepted the dynamic module during cyclic loading and the damaging tension reduced to an unit of body weight of the rabbit. As a result of the experimental biochemical studies they found that tendons, injected with corticosteroids, presented reduced strength and increased deformity in comparison with the noninjected tendons. Histologic examinations, performed additionaly, revealed that the functional properties of the tendons, injected with corticosteroids, were altered before the occurrence of structural changes in the tendinous tissue."}
{"id": "PMID:467313", "title": "[Comparison of the decurarizing and median lethal doses of 2-aminopyridine salts].", "content": "The authors determined LD50 of 2-aminopyridine hydrochloride, 2-aminopyridine jodmethylate, 2-aminopyridine methyl-para-toluolsulfonat and pymadine (4-aminopyridine hydrochloride) in mice and rats after subcutaneous administration. They found that doses of the same compounds during indirect stimulation of m. gastrocknemius removed myorelaxating action of (+)-tubocurarine. LD50 and decurarizing doses of jodmethylates and methyl-para-toluosulfonat were higher than LD50 of hydrochloride of 2-aminopyridine. When LD50 was compared with the decurarizing dose of 2-aminopyridine methyl-para-toluolslufonat, its lower toxicity and potential perspectivity for practical application as an anticurare agent was indicated.", "contents": "[Comparison of the decurarizing and median lethal doses of 2-aminopyridine salts]. The authors determined LD50 of 2-aminopyridine hydrochloride, 2-aminopyridine jodmethylate, 2-aminopyridine methyl-para-toluolsulfonat and pymadine (4-aminopyridine hydrochloride) in mice and rats after subcutaneous administration. They found that doses of the same compounds during indirect stimulation of m. gastrocknemius removed myorelaxating action of (+)-tubocurarine. LD50 and decurarizing doses of jodmethylates and methyl-para-toluosulfonat were higher than LD50 of hydrochloride of 2-aminopyridine. When LD50 was compared with the decurarizing dose of 2-aminopyridine methyl-para-toluolslufonat, its lower toxicity and potential perspectivity for practical application as an anticurare agent was indicated."}
{"id": "PMID:467325", "title": "Central nervous system action of bombesin: mechanism to induce hyperglycemia.", "content": "Bombesin acts within the brain to produce a prompt and sustained hyperglycemia, hyperglucagonemia, and relative or absolute hypoinsulinemia. Bombesin does not decrease plasma glucose turnover. Acute adrenalectomy but not hypophysectomy prevents hyperglycemia and hyperglucagonemia after intracisternal administration of bombesin. Administration of bombesin into the lateral ventricle of awake, unrestrained animals results in elevation of plasma glucose, preceded by a significant increase in plasma epinephrine and no increase in plasma norepinephrine or dopamine. Systemic administration of somatostatin prevents bombesin-induced hyperglycemia and hyperglucagonemia. These data support the conclusion that bombesin acts within the brain to increase sympathetic outflow resulting in increased adrenalmedullary epinephrine secretion, followed by depression of plasma insulin and elevation of plasma glucagon and glucose.", "contents": "Central nervous system action of bombesin: mechanism to induce hyperglycemia. Bombesin acts within the brain to produce a prompt and sustained hyperglycemia, hyperglucagonemia, and relative or absolute hypoinsulinemia. Bombesin does not decrease plasma glucose turnover. Acute adrenalectomy but not hypophysectomy prevents hyperglycemia and hyperglucagonemia after intracisternal administration of bombesin. Administration of bombesin into the lateral ventricle of awake, unrestrained animals results in elevation of plasma glucose, preceded by a significant increase in plasma epinephrine and no increase in plasma norepinephrine or dopamine. Systemic administration of somatostatin prevents bombesin-induced hyperglycemia and hyperglucagonemia. These data support the conclusion that bombesin acts within the brain to increase sympathetic outflow resulting in increased adrenalmedullary epinephrine secretion, followed by depression of plasma insulin and elevation of plasma glucagon and glucose."}
{"id": "PMID:467326", "title": "The hypothalamic-pituitary-gonadal axis during hyperthyroidism in the rat.", "content": "The rat, an animal without testosterone-estrogen-binding-globulin, was treated with L-T4 to the point of hyperthyroidism in order to study the hypothalamic-pituitary-testicular axis during this condition. Hyperthyroidism led to a significant decline in serum FSH, a fall in serum LH which was not satistically significant, and no change in serum levels of testosterone or estradiol. Testes of hyperthyroid rats produced significantly more testosterone during in vitro incubations than did the testes of control animals. We conclude that hyperthyroidism in the rat leads to a fall in FSH levels either via direct pituitary suppression or via accelerated FSH metabolism. In addition, in vitro studies suggest that excess thyroid hormone may stimulate intratesticular 17 beta-hydroxysteroid dehydrogenase.", "contents": "The hypothalamic-pituitary-gonadal axis during hyperthyroidism in the rat. The rat, an animal without testosterone-estrogen-binding-globulin, was treated with L-T4 to the point of hyperthyroidism in order to study the hypothalamic-pituitary-testicular axis during this condition. Hyperthyroidism led to a significant decline in serum FSH, a fall in serum LH which was not satistically significant, and no change in serum levels of testosterone or estradiol. Testes of hyperthyroid rats produced significantly more testosterone during in vitro incubations than did the testes of control animals. We conclude that hyperthyroidism in the rat leads to a fall in FSH levels either via direct pituitary suppression or via accelerated FSH metabolism. In addition, in vitro studies suggest that excess thyroid hormone may stimulate intratesticular 17 beta-hydroxysteroid dehydrogenase."}
{"id": "PMID:467329", "title": "Properties of nuclear and cytoplasmic estrogen receptor in the rabbit corpus luteum: evidence for translocation.", "content": "Nuclear and cytoplasmic estrogen receptors have been identified and characterized in the rabbit corpus luteum, and validated methods are described for the measurement of both unoccupied and total estrogen receptor. Binding was specific for the biologically active estrogens. Equilibrium binding analysis of cytoplasmic estrogen receptor yielded saturable, high affinity binding sites with a Kd of 5.4 x 10(-11) M. Two types of binding sites were found in the nuclear fraction: one with high affinity (Kd = 8.9 x 10(-11) M) and low capacity, the other with low affinity (Kd = 2.7 x 10(-8) M) and high capacity. In the presence of 0.4 M KCl, the sedimentation coefficients of nuclear and cytoplasmic estrogen receptors are 3.4S, while the value is 6.8S for cytoplasmic receptor in buffer without KCl. Twenty minutes after an iv injection of 2 micrograms 17 beta-estradiol, the available and total cytoplasmic estrogen receptors were depleted. This depletion was accompanied by concomitant and stoichiometric accumulation of receptor in the nucleus, indicating an apparent translocation of the receptor. In corpora lutea of normal rabbits, approximately 80% of the total nuclear receptor is unoccupied. Evidence is presented to suggest that nuclear receptor sites might be ordinarily occupied with estradiol, but during isolation of the nuclear fraction these sites become available or unoccupied. The identification of nuclear estrogen receptor and the phenomenon of translocation of cytoplasmic receptor to the nucleus suggest a similarity of estrogen action in the rabbit corpus luteum and other estrogen target tissues.", "contents": "Properties of nuclear and cytoplasmic estrogen receptor in the rabbit corpus luteum: evidence for translocation. Nuclear and cytoplasmic estrogen receptors have been identified and characterized in the rabbit corpus luteum, and validated methods are described for the measurement of both unoccupied and total estrogen receptor. Binding was specific for the biologically active estrogens. Equilibrium binding analysis of cytoplasmic estrogen receptor yielded saturable, high affinity binding sites with a Kd of 5.4 x 10(-11) M. Two types of binding sites were found in the nuclear fraction: one with high affinity (Kd = 8.9 x 10(-11) M) and low capacity, the other with low affinity (Kd = 2.7 x 10(-8) M) and high capacity. In the presence of 0.4 M KCl, the sedimentation coefficients of nuclear and cytoplasmic estrogen receptors are 3.4S, while the value is 6.8S for cytoplasmic receptor in buffer without KCl. Twenty minutes after an iv injection of 2 micrograms 17 beta-estradiol, the available and total cytoplasmic estrogen receptors were depleted. This depletion was accompanied by concomitant and stoichiometric accumulation of receptor in the nucleus, indicating an apparent translocation of the receptor. In corpora lutea of normal rabbits, approximately 80% of the total nuclear receptor is unoccupied. Evidence is presented to suggest that nuclear receptor sites might be ordinarily occupied with estradiol, but during isolation of the nuclear fraction these sites become available or unoccupied. The identification of nuclear estrogen receptor and the phenomenon of translocation of cytoplasmic receptor to the nucleus suggest a similarity of estrogen action in the rabbit corpus luteum and other estrogen target tissues."}
{"id": "PMID:467331", "title": "Effect of anterior hypothalamic lesions on neurohypophysial and peripheral tissue concentrations of somatostatin in the rat.", "content": "Somatostatin (SRIF) is present in nerve endings in the median eminence (ME) and posterior pituitary. Hypothalamic SRIF containing neuronal perikarya are predominantly located in the anterior hypothalamic area (AHA), a region implicated in the inhibitory control of GH secretion. The effect of AHA lesions on SRIF in the ME, posterior pituitary, pancreas, stomach, and small intestine was studied in the rat in order to elucidate the source of ME and posterior pituitary SRIF and to determine if depletion of hypothalamic SRIF affects peripheral organ concentrations of the peptide. Lesioned animals showed a highly significant (P less than 0.01) 83% and 82% reduction in ME and posterior pituitary SRIF and to determine if depletion of hypothalamic SRIF affects peripheral organ concentrations of the peptide. Lesioned animals showed a highly significant (P less than SRIF concentrations in the pancreas, stomach, and small intestine of the lesioned animals. Plasma and pancreatic insulin and pancreatic glucagon were likewise unchanged. These data suggest that the hypothalamic SRIF pathway begins in the AHA, from where axons of somatostatinergic neurosecretory neurons project to both ME and posterior pituitary. AHA lesions have no effect on gut and pancreatic SRIF or pancreatic insulin and glucagon.", "contents": "Effect of anterior hypothalamic lesions on neurohypophysial and peripheral tissue concentrations of somatostatin in the rat. Somatostatin (SRIF) is present in nerve endings in the median eminence (ME) and posterior pituitary. Hypothalamic SRIF containing neuronal perikarya are predominantly located in the anterior hypothalamic area (AHA), a region implicated in the inhibitory control of GH secretion. The effect of AHA lesions on SRIF in the ME, posterior pituitary, pancreas, stomach, and small intestine was studied in the rat in order to elucidate the source of ME and posterior pituitary SRIF and to determine if depletion of hypothalamic SRIF affects peripheral organ concentrations of the peptide. Lesioned animals showed a highly significant (P less than 0.01) 83% and 82% reduction in ME and posterior pituitary SRIF and to determine if depletion of hypothalamic SRIF affects peripheral organ concentrations of the peptide. Lesioned animals showed a highly significant (P less than SRIF concentrations in the pancreas, stomach, and small intestine of the lesioned animals. Plasma and pancreatic insulin and pancreatic glucagon were likewise unchanged. These data suggest that the hypothalamic SRIF pathway begins in the AHA, from where axons of somatostatinergic neurosecretory neurons project to both ME and posterior pituitary. AHA lesions have no effect on gut and pancreatic SRIF or pancreatic insulin and glucagon."}
{"id": "PMID:467334", "title": "Growth of pancreas and gastrointestinal mucosa in antrectomized and gastrin-treated rats.", "content": "Growth responses to endogenous and exogenous gastrin were examined in the pancreas and gastrointestinal tract mucosa. Rats were either antrectomized to remove the primary source of endogenous gastrin or subjected to a sham operation. Three weeks after surgery, half of the antrectomized animals were injected ip with pentagastrin (250 microgram/day) four times per day. Injections were carried out for a week. Antrectomy resulted in serum gastrin levels approximately one third of normal. DNA synthesis and DNA and RNA content of the pancreas and oxyntic gland, duodenal, and colonic mucosa were significantly reduced by antrectomy. In each case, pentagastrin treatment restored DNA synthesis and RNA and DNA levels to normal. Significant decreases in pancreatic and colonic weights in antrectomized animals were also completely prevented by pentagastrin injection. These results indicate that endogenous gastrin has an important role in the regulation of pancreatic and colonic mucosal growth, in addition to its already established similar function in oxyntic gland mucosa.", "contents": "Growth of pancreas and gastrointestinal mucosa in antrectomized and gastrin-treated rats. Growth responses to endogenous and exogenous gastrin were examined in the pancreas and gastrointestinal tract mucosa. Rats were either antrectomized to remove the primary source of endogenous gastrin or subjected to a sham operation. Three weeks after surgery, half of the antrectomized animals were injected ip with pentagastrin (250 microgram/day) four times per day. Injections were carried out for a week. Antrectomy resulted in serum gastrin levels approximately one third of normal. DNA synthesis and DNA and RNA content of the pancreas and oxyntic gland, duodenal, and colonic mucosa were significantly reduced by antrectomy. In each case, pentagastrin treatment restored DNA synthesis and RNA and DNA levels to normal. Significant decreases in pancreatic and colonic weights in antrectomized animals were also completely prevented by pentagastrin injection. These results indicate that endogenous gastrin has an important role in the regulation of pancreatic and colonic mucosal growth, in addition to its already established similar function in oxyntic gland mucosa."}
{"id": "PMID:467335", "title": "Dexamethasone effect on the phospholipid content of isolated fat cell ghosts from adrenalectomized rats.", "content": "The lipid composition of sonicated ghosts from isolated fat cells has been investigated. Epididymal fat cells from adrenalectomized rats were incubated with and without 8 x 10(-8) M dexamethasone for 3 h. Phospholipids and cholesterol were isolated from membrane ghosts prepared from these cells. There was a 61% increase in membrane sphingomyelin from cells incubated with dexamethasone (P less than 0.025). No significant change was observed in other phospholipids, cholesterol, or fatty acid components of the phospholipids.", "contents": "Dexamethasone effect on the phospholipid content of isolated fat cell ghosts from adrenalectomized rats. The lipid composition of sonicated ghosts from isolated fat cells has been investigated. Epididymal fat cells from adrenalectomized rats were incubated with and without 8 x 10(-8) M dexamethasone for 3 h. Phospholipids and cholesterol were isolated from membrane ghosts prepared from these cells. There was a 61% increase in membrane sphingomyelin from cells incubated with dexamethasone (P less than 0.025). No significant change was observed in other phospholipids, cholesterol, or fatty acid components of the phospholipids."}
{"id": "PMID:467337", "title": "Actions of insulin and hydrocortisone on macromolecular synthesis in primary epithelial cell cultures from mouse mammary glands.", "content": "Monolayer cultures of mammary gland epithelial cells were prepared from the abdominal glands of midpregnancy mice. After collagenase digestion of mammary tissue and separation by differential centrifugation, the isolated epithelial cells were cultured in Eagle's Minimal Essential Medium supplemented with 10% fetal bovine serum and insulin (6 micrograms/ml). Six days later, when the cultures were in log growth and nearly confluent, the effects of insulin and/or hydrocortisone on the rates of RNA, DNA, and protein synthesis were determined in a serum-free medium. At physiological concentrations, insulin enhanced the rates of uptake and incorporation of [3H]uridine into RNA, of [3H]thymidine into DNA, and of [3H]leucine into protein. Hydrocortisone was shown to be biphasic with regard to concentration in attenuating or augmenting insulin's effects on macromolecular synthesis.", "contents": "Actions of insulin and hydrocortisone on macromolecular synthesis in primary epithelial cell cultures from mouse mammary glands. Monolayer cultures of mammary gland epithelial cells were prepared from the abdominal glands of midpregnancy mice. After collagenase digestion of mammary tissue and separation by differential centrifugation, the isolated epithelial cells were cultured in Eagle's Minimal Essential Medium supplemented with 10% fetal bovine serum and insulin (6 micrograms/ml). Six days later, when the cultures were in log growth and nearly confluent, the effects of insulin and/or hydrocortisone on the rates of RNA, DNA, and protein synthesis were determined in a serum-free medium. At physiological concentrations, insulin enhanced the rates of uptake and incorporation of [3H]uridine into RNA, of [3H]thymidine into DNA, and of [3H]leucine into protein. Hydrocortisone was shown to be biphasic with regard to concentration in attenuating or augmenting insulin's effects on macromolecular synthesis."}
{"id": "PMID:467338", "title": "No evidence for the existence of a species-specific renin substrate in plasma from normal and bilaterally nephrectomized rats.", "content": "Renin substrate in plasma from normal and biolaterally nephrectomized rats was measured using an excess of rat or rabbit renin which had the same pressor activity when directly assayed in the rat. The amounts of angiotensin I generated with an excess of rat renin were similar to those generated with an excess of rabbit renin in plasma from normal and bilaterally nephrectomized rats. Further addition of an excess of homologous renin to the incubation mixture did not generate more angiotensin I from normal and bilaterally nephrectomized rat plasma which had been incubated before with an excess of rabbit renin. The isoelectric focusing profiles of plasma renin substrate from normal and bilaterally nephrectomized rats were almost identical using an excess of either rat or rabbit renin. It is concluded that there is no species-specific renin substrate for homologous renin in normal or bilaterally nephrectomized rats.", "contents": "No evidence for the existence of a species-specific renin substrate in plasma from normal and bilaterally nephrectomized rats. Renin substrate in plasma from normal and biolaterally nephrectomized rats was measured using an excess of rat or rabbit renin which had the same pressor activity when directly assayed in the rat. The amounts of angiotensin I generated with an excess of rat renin were similar to those generated with an excess of rabbit renin in plasma from normal and bilaterally nephrectomized rats. Further addition of an excess of homologous renin to the incubation mixture did not generate more angiotensin I from normal and bilaterally nephrectomized rat plasma which had been incubated before with an excess of rabbit renin. The isoelectric focusing profiles of plasma renin substrate from normal and bilaterally nephrectomized rats were almost identical using an excess of either rat or rabbit renin. It is concluded that there is no species-specific renin substrate for homologous renin in normal or bilaterally nephrectomized rats."}
{"id": "PMID:467339", "title": "Independent inhibition of prolactin secretion by dopamine and gamma-aminobutyric acid in vitro.", "content": "gamma-Aminobutyric acid (GABA) inhibits PRL release from incubated hemipituitaries in a dose-dependent manner. The maximum inhibition obtained with GABA is less than that obtained with dopamine. Its affinity is 100 times lower. The effect is blocked by picrotoxin but not by a dopamine inhibitor; alpha-flupentixol but not picrotoxin antagonizes dopamine inhibition. This indicates that dopamine and GABA inhibit PRL release through independent receptors. The hypothalamic extract contains sufficient GABA to inhibit PRL release in our in vitro conditions. Picrotoxin, however, does not significantly inhibit the nondopaminergic PRL-inhibiting activity of mediobasal hypothalamic extracts. Another nondopaminergic PRL-inhibiting factor, therefore, seems to be present in the hypothalamus.", "contents": "Independent inhibition of prolactin secretion by dopamine and gamma-aminobutyric acid in vitro. gamma-Aminobutyric acid (GABA) inhibits PRL release from incubated hemipituitaries in a dose-dependent manner. The maximum inhibition obtained with GABA is less than that obtained with dopamine. Its affinity is 100 times lower. The effect is blocked by picrotoxin but not by a dopamine inhibitor; alpha-flupentixol but not picrotoxin antagonizes dopamine inhibition. This indicates that dopamine and GABA inhibit PRL release through independent receptors. The hypothalamic extract contains sufficient GABA to inhibit PRL release in our in vitro conditions. Picrotoxin, however, does not significantly inhibit the nondopaminergic PRL-inhibiting activity of mediobasal hypothalamic extracts. Another nondopaminergic PRL-inhibiting factor, therefore, seems to be present in the hypothalamus."}
{"id": "PMID:467343", "title": "Serum somatomedin activity and cartilage metabolism in acutely fasted, chronically malnourished, and refed rats.", "content": "Serum somatomedin activity (SM-act) and cartilage metabolism were compared in acutely fasted, marasmic (M), and marasmic kwashiorkor (MK) rats. SM-act was estimated in the porcine bioassay. In vitro uptake of [35S]sulfate and [3H]methylthymidine in costal cartilage of the experimental animals during an incubation in medium immediately after sacrifice, called endogenous activity, and the effect of incubation in 20% normal human plasma after a preincubation of 22 h in medium only, called plasma responsiveness\", were determined. Acutely fasted rats had lowered SM-act and a circulating heat-labile inhibitor. Endogenous activity and responsiveness of cartilage were depressed. MK rats (fed ad libitum a 0.5% casein, isocaloric food) showed a profound depression of growth and cartilage endogenous activity despite only partially reduced SM-act and increased responsiveness. M rats received normal food and were pair-fed with MK rats, consuming approximately 0.08 g/g BW . day. They showed very depressed SM-act and low endogenous activity, and responsiveness was increased, though less than in the MK rats. On refeeding M rats, SM-act and cartilage responsiveness increased, followed by an increase of endogenous activity. Catch-up growth was best related to [3H]methylthymidine incorporation by cartilage (endogenous activity). In conclusion, these two types of experimental chronic malnutrition induce a more diversified pattern than does acute fasting. During malnutrition, cartilage metabolism does not reflect bioassayable SM-act of serum but rather the other effects of the nutritional insult. On refeeding, the expected relationship of SM-act and cartilage metabolism is rapidly restored.", "contents": "Serum somatomedin activity and cartilage metabolism in acutely fasted, chronically malnourished, and refed rats. Serum somatomedin activity (SM-act) and cartilage metabolism were compared in acutely fasted, marasmic (M), and marasmic kwashiorkor (MK) rats. SM-act was estimated in the porcine bioassay. In vitro uptake of [35S]sulfate and [3H]methylthymidine in costal cartilage of the experimental animals during an incubation in medium immediately after sacrifice, called endogenous activity, and the effect of incubation in 20% normal human plasma after a preincubation of 22 h in medium only, called plasma responsiveness\", were determined. Acutely fasted rats had lowered SM-act and a circulating heat-labile inhibitor. Endogenous activity and responsiveness of cartilage were depressed. MK rats (fed ad libitum a 0.5% casein, isocaloric food) showed a profound depression of growth and cartilage endogenous activity despite only partially reduced SM-act and increased responsiveness. M rats received normal food and were pair-fed with MK rats, consuming approximately 0.08 g/g BW . day. They showed very depressed SM-act and low endogenous activity, and responsiveness was increased, though less than in the MK rats. On refeeding M rats, SM-act and cartilage responsiveness increased, followed by an increase of endogenous activity. Catch-up growth was best related to [3H]methylthymidine incorporation by cartilage (endogenous activity). In conclusion, these two types of experimental chronic malnutrition induce a more diversified pattern than does acute fasting. During malnutrition, cartilage metabolism does not reflect bioassayable SM-act of serum but rather the other effects of the nutritional insult. On refeeding, the expected relationship of SM-act and cartilage metabolism is rapidly restored."}
{"id": "PMID:467345", "title": "Changes in sex hormones and calcium regulating hormones with reference to bone mass associated with aging.", "content": "Measurement of bone mineral content (BMC), intestinal 47Ca absorption, and calcium regulating hormones and sex steroids in serum were performed on 32 healthy aged subjects and 26 control young subjects. In BMC, there was a progressive fall after age 40, with the rate of decrease being greater in women than in men. A significant correlation was observed between BMC and testosterone in the men and between estrogens and BMC in the women, suggesting the possible importance of testosterone in men and estrogens in women in maintaining bone mass. Plasma PTH showed no change with age. However, the reserve capacity of the parathyroid was significantly reduced in the aged women. Serum levels of ionized calcium were low in aged subjects, indicating a possible alteration with age in the feedback control between ionized calcium levels and parathyroid hormone secretion. C-cell funtion was also decreased with age. Plasma 1,25-(OH)2D and 47Ca absorption tended to decrease with age. Age-related bone loss could be a reflection of the interaction of these hormonal imbalance occurring with age.", "contents": "Changes in sex hormones and calcium regulating hormones with reference to bone mass associated with aging. Measurement of bone mineral content (BMC), intestinal 47Ca absorption, and calcium regulating hormones and sex steroids in serum were performed on 32 healthy aged subjects and 26 control young subjects. In BMC, there was a progressive fall after age 40, with the rate of decrease being greater in women than in men. A significant correlation was observed between BMC and testosterone in the men and between estrogens and BMC in the women, suggesting the possible importance of testosterone in men and estrogens in women in maintaining bone mass. Plasma PTH showed no change with age. However, the reserve capacity of the parathyroid was significantly reduced in the aged women. Serum levels of ionized calcium were low in aged subjects, indicating a possible alteration with age in the feedback control between ionized calcium levels and parathyroid hormone secretion. C-cell funtion was also decreased with age. Plasma 1,25-(OH)2D and 47Ca absorption tended to decrease with age. Age-related bone loss could be a reflection of the interaction of these hormonal imbalance occurring with age."}
{"id": "PMID:467347", "title": "Postmenopausal and senile osteoporosis: current concepts of etiology and treatment.", "content": "Postmenopausal osteoporosis and senile osteoporosis are heterogeneous disorders that appear to be caused by several nonhormonal and hormonal factors. Of nonhormonal factors, age-related bone loss, the degree of bone density achieved in young adult life, and dietary intake and absorption of calcium appear to be important. Hormones that may be important in pathogenesis are parathyroid hormone (PTH), estrogen, 1, 25(OH)2D, and possibly calcitonin. Postmenopausal estrogen deficiency accelerates bone loss by increasing responsiveness of bone to endogenous PTH. The resultant increase in release of calcium from bone is associated with normal or low values for serum immunoreactive PTH (iPTH) (except for a subset of 15% of the total which have high values and appear to represent a separate population). Some evidence suggests that subnormal calcium absorption, which is a common finding in postmenopausal osteoporosis and which may contribute to negative calcium balance, is caused by decreased conversion of 25-OH-D to 1,25(OH)2D. Treatment of osteoporosis with either sex steroids (by antagonizing the effect of PTH on bone resorption) or orally administered calcium with or without vitamin D (by decreasing PTH secretion) decreases bone resorption. Long-term therapy with these agents, however, decreases bone formation; thus, bone loss is only arrested or slowed. Although combined therapy with fluoride and calcium stimulates bone formation and appears to be capable of increasing bone mass, its long-term safety and efficacy in decreasing the the occurrence of fractures remain to be demonstrated.", "contents": "Postmenopausal and senile osteoporosis: current concepts of etiology and treatment. Postmenopausal osteoporosis and senile osteoporosis are heterogeneous disorders that appear to be caused by several nonhormonal and hormonal factors. Of nonhormonal factors, age-related bone loss, the degree of bone density achieved in young adult life, and dietary intake and absorption of calcium appear to be important. Hormones that may be important in pathogenesis are parathyroid hormone (PTH), estrogen, 1, 25(OH)2D, and possibly calcitonin. Postmenopausal estrogen deficiency accelerates bone loss by increasing responsiveness of bone to endogenous PTH. The resultant increase in release of calcium from bone is associated with normal or low values for serum immunoreactive PTH (iPTH) (except for a subset of 15% of the total which have high values and appear to represent a separate population). Some evidence suggests that subnormal calcium absorption, which is a common finding in postmenopausal osteoporosis and which may contribute to negative calcium balance, is caused by decreased conversion of 25-OH-D to 1,25(OH)2D. Treatment of osteoporosis with either sex steroids (by antagonizing the effect of PTH on bone resorption) or orally administered calcium with or without vitamin D (by decreasing PTH secretion) decreases bone resorption. Long-term therapy with these agents, however, decreases bone formation; thus, bone loss is only arrested or slowed. Although combined therapy with fluoride and calcium stimulates bone formation and appears to be capable of increasing bone mass, its long-term safety and efficacy in decreasing the the occurrence of fractures remain to be demonstrated."}
{"id": "PMID:467348", "title": "Treatment of osteoporosis--with special reference to the coexistence of osteoporosis and osteomalacia.", "content": "1. The current status of treatment for osteoporosis at the osteoporosis clinic of the Department of Orthopaedic Surgery, Kobe University School of Medicine is reported. A study was made in these osteoporotic patients to investigate the relationships between pain and x-rays and also between pain and the clinical laboratory findings in this particular disease. 2. When pains in osteoporosis were classified into 4 grades, severity I through severity IV, the more severe pain was found to be associated more frequently with elevated blood levels of alkaline phosphatase. Many of the patients with severe pain tended to show indistinct trabeculation and sclerosis of the upper and lower margins of vertebral bodies on x-rays while some demonstrated looser's zones notably in the ribs. 3. A considerable portion of those patients who were receiving treatment under the diagnosis of osteoporosis were found to have evidence of osteomalacia, a finding pointing to the likelihood of coexistence of osteoporosis and osteomalacia. This possibility should therefore always be kept in mind when dealing with osteoporotic patients.", "contents": "Treatment of osteoporosis--with special reference to the coexistence of osteoporosis and osteomalacia. 1. The current status of treatment for osteoporosis at the osteoporosis clinic of the Department of Orthopaedic Surgery, Kobe University School of Medicine is reported. A study was made in these osteoporotic patients to investigate the relationships between pain and x-rays and also between pain and the clinical laboratory findings in this particular disease. 2. When pains in osteoporosis were classified into 4 grades, severity I through severity IV, the more severe pain was found to be associated more frequently with elevated blood levels of alkaline phosphatase. Many of the patients with severe pain tended to show indistinct trabeculation and sclerosis of the upper and lower margins of vertebral bodies on x-rays while some demonstrated looser's zones notably in the ribs. 3. A considerable portion of those patients who were receiving treatment under the diagnosis of osteoporosis were found to have evidence of osteomalacia, a finding pointing to the likelihood of coexistence of osteoporosis and osteomalacia. This possibility should therefore always be kept in mind when dealing with osteoporotic patients."}
{"id": "PMID:467349", "title": "Biological activity of 1-alpha-hydroxy vitamin D3 in vitamin D dependency.", "content": "The biological activity of 1(OH) vitamin D3 was evaluated in 2 cases of vitamin D dependency. For the improvement of serum chemistry and increment of urinary excretion of calcium 1(OH) vitamin D3 was 500--1000 times more active than vitamin D2. The administration of 0.05 microg/kg/day and 0.1 microg/kg/day of 1(OH) vitamin D3 were equally effective to keep the serum chemistry within normal limits. However, urinary excretion of calcium increased to an abnormal height on 0.1 microg/kg/day of 1(OH) vitamin D3. On the other hand, administration of 0.05 microg/kg/day of 1(OH) vitamin D3 kept both serum level and urinary excretion of calcium normal. It is suggested that 0.05 microg/kg/day is close to the optimum requirement of 1(OH) vitamin D3 in vitamin D dependency and this should correspond to the essential requirement of 1(OH) vitamin D3 in man.", "contents": "Biological activity of 1-alpha-hydroxy vitamin D3 in vitamin D dependency. The biological activity of 1(OH) vitamin D3 was evaluated in 2 cases of vitamin D dependency. For the improvement of serum chemistry and increment of urinary excretion of calcium 1(OH) vitamin D3 was 500--1000 times more active than vitamin D2. The administration of 0.05 microg/kg/day and 0.1 microg/kg/day of 1(OH) vitamin D3 were equally effective to keep the serum chemistry within normal limits. However, urinary excretion of calcium increased to an abnormal height on 0.1 microg/kg/day of 1(OH) vitamin D3. On the other hand, administration of 0.05 microg/kg/day of 1(OH) vitamin D3 kept both serum level and urinary excretion of calcium normal. It is suggested that 0.05 microg/kg/day is close to the optimum requirement of 1(OH) vitamin D3 in vitamin D dependency and this should correspond to the essential requirement of 1(OH) vitamin D3 in man."}
{"id": "PMID:467350", "title": "Osteomalacic myopathy.", "content": "Muscles from two cases of osteomalacia were studied histochemically and electron-microscopically. Histopathological finding were common in these two cases. There are (1) myopathic changes such as scattered muscle fiber atrophy, necrosis, and internal uuclei, (2) derangement of intermyofibrillar network. and (3) type II fiber atrophy. Electron-microscopical finding corresponded well with light microscopical findings. These are distinct pathological features and deserves to be called osteomalacic myopathy. As the pathogenetic mechanism of this myopathy, phosphate depletion in the muscle cells resulting in disturbed glycolysis, and decreased vitamin D effects on muscle cells resulting in diminished calcium uptake by sarcoplasmic reticulum, are considered to be the most important two factors.", "contents": "Osteomalacic myopathy. Muscles from two cases of osteomalacia were studied histochemically and electron-microscopically. Histopathological finding were common in these two cases. There are (1) myopathic changes such as scattered muscle fiber atrophy, necrosis, and internal uuclei, (2) derangement of intermyofibrillar network. and (3) type II fiber atrophy. Electron-microscopical finding corresponded well with light microscopical findings. These are distinct pathological features and deserves to be called osteomalacic myopathy. As the pathogenetic mechanism of this myopathy, phosphate depletion in the muscle cells resulting in disturbed glycolysis, and decreased vitamin D effects on muscle cells resulting in diminished calcium uptake by sarcoplasmic reticulum, are considered to be the most important two factors."}
{"id": "PMID:467351", "title": "Vitamin D and osteoporosis.", "content": "In elderly people with marginal exposure to the sunlight, males showed higher serum 25-hydroxycalciferol than females, whereas in those with ample or poor sunlight exposure, serum 25-hydroxycalciferol was higher or very low, respectively, exhibiting no sex difference in the vitamin D metabolite levels. The male predominance in serum 25-hydroxycalciferol levels seen among some aged population would be explained, at least in part, by the result of animal experiment suggesting the stimulatory effect of testosterone on vitamin D biosynthesis induced by ultaviolet irradiation. Testosterone was, furthermore, shown to have hypocalcemic action, probably through suppression of bone resortopton in vitamin D depleted but not in replete rats. Clinical implication of these two-fold effects of testosterone observed in rats was discussed in relevance to male predominance in serum 25-hydroxycalciferol level and bone mineral content in the aged population.", "contents": "Vitamin D and osteoporosis. In elderly people with marginal exposure to the sunlight, males showed higher serum 25-hydroxycalciferol than females, whereas in those with ample or poor sunlight exposure, serum 25-hydroxycalciferol was higher or very low, respectively, exhibiting no sex difference in the vitamin D metabolite levels. The male predominance in serum 25-hydroxycalciferol levels seen among some aged population would be explained, at least in part, by the result of animal experiment suggesting the stimulatory effect of testosterone on vitamin D biosynthesis induced by ultaviolet irradiation. Testosterone was, furthermore, shown to have hypocalcemic action, probably through suppression of bone resortopton in vitamin D depleted but not in replete rats. Clinical implication of these two-fold effects of testosterone observed in rats was discussed in relevance to male predominance in serum 25-hydroxycalciferol level and bone mineral content in the aged population."}
{"id": "PMID:467352", "title": "Effect of 1 alpha-hydroxyvitamin D3 in rats with experimental renal osteodystrophy.", "content": "A model of experimental renal osteodystrophy was established in the rats with chronic renal failure induced by partial nephrectomy and therapeutic effects of 1 alpha-hydroxyvitamin D3 (1 alpha-OH-D3) were studied. Male Wistar rats weighing 180 g were 5/6 nephrectomized and fed a normal diet (Ca and P : 1%) for 6 months. After the surgery, serum creatinine levels increased 60% and thereafter continued to rise gradually with their growth for 4 to 5 months, followed by rapid increase. The serum phosphorus levels were also elevated concomitantly and the serum calcium concentrations were normal. Marked bone resorption accompanied with hypertrophy of parathyroid glands was observed by histological examinations (Tetrachrome-Fuchsin stain, contact microradiography and H-E stain). The bone resorption seemed to be due to secondary hyperparathyroidism. Treatment with 0.25 micrograms/kg/day p.o. of 1 alpha-OH-D3 for 10 days in the uremic state resulted in remarkable new bone formation which was confirmed by histological examinations. These results clearly demonstrated that the reduction of nephron mass play a critical clue of renal osteodystrophy and 1 alpha-OH-D3 appears to have a good potential for clinical use in patients with renal failure and metabolic bone diseases.", "contents": "Effect of 1 alpha-hydroxyvitamin D3 in rats with experimental renal osteodystrophy. A model of experimental renal osteodystrophy was established in the rats with chronic renal failure induced by partial nephrectomy and therapeutic effects of 1 alpha-hydroxyvitamin D3 (1 alpha-OH-D3) were studied. Male Wistar rats weighing 180 g were 5/6 nephrectomized and fed a normal diet (Ca and P : 1%) for 6 months. After the surgery, serum creatinine levels increased 60% and thereafter continued to rise gradually with their growth for 4 to 5 months, followed by rapid increase. The serum phosphorus levels were also elevated concomitantly and the serum calcium concentrations were normal. Marked bone resorption accompanied with hypertrophy of parathyroid glands was observed by histological examinations (Tetrachrome-Fuchsin stain, contact microradiography and H-E stain). The bone resorption seemed to be due to secondary hyperparathyroidism. Treatment with 0.25 micrograms/kg/day p.o. of 1 alpha-OH-D3 for 10 days in the uremic state resulted in remarkable new bone formation which was confirmed by histological examinations. These results clearly demonstrated that the reduction of nephron mass play a critical clue of renal osteodystrophy and 1 alpha-OH-D3 appears to have a good potential for clinical use in patients with renal failure and metabolic bone diseases."}
{"id": "PMID:467353", "title": "Age-related changes of renal osteodystrophy.", "content": "Bone changes in 138 patients under hemodialysis were evaluated by radiological methods. Bone mass as was assessed by clavicular score was reduced in patients with chronic renal failure compared with control subjects, especially in male patients, and showed the gradual decrease with aging in both sexes. The incidence of disappearance of lamina dura and that of vascular calcification increased with aging in chronic renal failure. Although serum PTH did not show much difference among various age groups, serum CT and calcium were significantly elevated in 20--24 years of age group compared with other age groups. The etiology of decrease of bone mass in male subjects was discussed from the standpoint of decreased gonadal activity.", "contents": "Age-related changes of renal osteodystrophy. Bone changes in 138 patients under hemodialysis were evaluated by radiological methods. Bone mass as was assessed by clavicular score was reduced in patients with chronic renal failure compared with control subjects, especially in male patients, and showed the gradual decrease with aging in both sexes. The incidence of disappearance of lamina dura and that of vascular calcification increased with aging in chronic renal failure. Although serum PTH did not show much difference among various age groups, serum CT and calcium were significantly elevated in 20--24 years of age group compared with other age groups. The etiology of decrease of bone mass in male subjects was discussed from the standpoint of decreased gonadal activity."}
{"id": "PMID:467354", "title": "Azotemic renal osteodystrophy; clinical features and bone pathology.", "content": "During the hemodialysis treatment of 543 uremic patients for 10 years, significant complications concerning metabolic calcium disturbances, subperiosteal resorption, calcification of soft tissue or peripheral vessels, and fractures were noted. Significant elevation of alkaline phosphatase was induced for 5 years under hemodialysis using 5.0--6.0 mg/dl dialysate calcium, but not under 7.5 mg/dl dialysate calcium. Plasma immunoreactive parathyroid hormone (iPTH) values were abnormally high with a few exceptions, without relationship to serum calcium levels. Among the patients with chronic renal failure on dietary control, osteomalacic changes were predominant, but their iPTH values were not always elevated. When the patients were treated for a long time with hemodialysis, the mixed type of osteomalacia and osteites fibrosa appeared. Administration of dihydrotachysterol and 1 alpha-hydroxycholecalciferol to the patients on hemodialysis changed the mixed type of osteomalacia and osteitis fibrosa to the osteomalacic type with the marked reduction of osteoid seam thickness.", "contents": "Azotemic renal osteodystrophy; clinical features and bone pathology. During the hemodialysis treatment of 543 uremic patients for 10 years, significant complications concerning metabolic calcium disturbances, subperiosteal resorption, calcification of soft tissue or peripheral vessels, and fractures were noted. Significant elevation of alkaline phosphatase was induced for 5 years under hemodialysis using 5.0--6.0 mg/dl dialysate calcium, but not under 7.5 mg/dl dialysate calcium. Plasma immunoreactive parathyroid hormone (iPTH) values were abnormally high with a few exceptions, without relationship to serum calcium levels. Among the patients with chronic renal failure on dietary control, osteomalacic changes were predominant, but their iPTH values were not always elevated. When the patients were treated for a long time with hemodialysis, the mixed type of osteomalacia and osteites fibrosa appeared. Administration of dihydrotachysterol and 1 alpha-hydroxycholecalciferol to the patients on hemodialysis changed the mixed type of osteomalacia and osteitis fibrosa to the osteomalacic type with the marked reduction of osteoid seam thickness."}
{"id": "PMID:467355", "title": "Influence of thyroid hormones on collagen content in tissues of guinea pigs.", "content": "Collagen fractions content and level of collagen catabolites in body fluids were determined in normal, hypo- and hyperthyroid guinea pigs. An increase of urinary excretion of hydroxyproline and hydroxylysine as well as concentration of these amino acids in blood serum was found in hyperthyroidism, and a decrease was shown in hyperthyroid guinea pigs. Hyperthyroidism stimulated an increase of neutral-salt-soluble and acid-soluble collagen in skin and liver, and a decrease of insoluble collagen in skin as well as increase of all collagen fractions in bone samples. Hypothyroidism induced a decrease of all collagen fractions in skin and liver, and an increase of acid-soluble and insoluble collagen in bone samples.", "contents": "Influence of thyroid hormones on collagen content in tissues of guinea pigs. Collagen fractions content and level of collagen catabolites in body fluids were determined in normal, hypo- and hyperthyroid guinea pigs. An increase of urinary excretion of hydroxyproline and hydroxylysine as well as concentration of these amino acids in blood serum was found in hyperthyroidism, and a decrease was shown in hyperthyroid guinea pigs. Hyperthyroidism stimulated an increase of neutral-salt-soluble and acid-soluble collagen in skin and liver, and a decrease of insoluble collagen in skin as well as increase of all collagen fractions in bone samples. Hypothyroidism induced a decrease of all collagen fractions in skin and liver, and an increase of acid-soluble and insoluble collagen in bone samples."}
{"id": "PMID:467356", "title": "[Secretion of growth hormone in pigs].", "content": "Spontaneous rhythmic changes of growth hormone concentration were observed in plasma of pigs, ranging between 30 and more than 100% as compared to the initial values. These rhythmic changes should be considered when plasma GH is determined.", "contents": "[Secretion of growth hormone in pigs]. Spontaneous rhythmic changes of growth hormone concentration were observed in plasma of pigs, ranging between 30 and more than 100% as compared to the initial values. These rhythmic changes should be considered when plasma GH is determined."}
{"id": "PMID:467357", "title": "Complex cytophotometric analysis of hormone-induced alterations in the glucose utilisation of Tetrahymena.", "content": "Insulin and adrenaline were both found to stimulate the glucose utilization of Tetrahymena, although somewhat differently. With exogenous sugar added, adrenaline always drops the glucose level in all regions of the unicellular, while the effect of insulin was less unequivocal.", "contents": "Complex cytophotometric analysis of hormone-induced alterations in the glucose utilisation of Tetrahymena. Insulin and adrenaline were both found to stimulate the glucose utilization of Tetrahymena, although somewhat differently. With exogenous sugar added, adrenaline always drops the glucose level in all regions of the unicellular, while the effect of insulin was less unequivocal."}
{"id": "PMID:467358", "title": "[Inhibition of ovulation with 17-alpha-ethinyl-18-methyl-11-methylene-4-estren-17-ol plus ethinylestradiol].", "content": "A combined oral application of 30 micrograms ethinylestradiol with 50 micrograms 17 alpha-ethinyl-18-methyl-11-methylene-4-estren-17-ol (a newly developed progestagen) -- for 21 days resulted in a distinct antigonadotrophic effect, shown by hormone analysis (LH, FSH, estradiol and progesterone) in serum samples of three healthy and fertile women. A final recommendation on the daily dosage, however, depends on the results of clinical experimental investigation still in progress.", "contents": "[Inhibition of ovulation with 17-alpha-ethinyl-18-methyl-11-methylene-4-estren-17-ol plus ethinylestradiol]. A combined oral application of 30 micrograms ethinylestradiol with 50 micrograms 17 alpha-ethinyl-18-methyl-11-methylene-4-estren-17-ol (a newly developed progestagen) -- for 21 days resulted in a distinct antigonadotrophic effect, shown by hormone analysis (LH, FSH, estradiol and progesterone) in serum samples of three healthy and fertile women. A final recommendation on the daily dosage, however, depends on the results of clinical experimental investigation still in progress."}
{"id": "PMID:467359", "title": "[Effect of 17-alpha-hydroxyprogesteronecapronate on sodium excretion and the renin-angiotensin-aldosterone-system in humans].", "content": "In seven healthy male subjects, a natriuretic effect of 17 alpha-hydroxyprogesterone caproate (17 alpha-OHPC) was demonstrated. Three of these subjects were kept on an uncontrolled diet and were examined over a period of 12 days. To the remaining four subjects, a single dose of 250 mg 17 alpha-OHPC was given intramuscularly after four days of intake of a chemically defined diet (Vivasorb). In this second test procedure, blood samples were taken in the recumbent position every two hours throughout a period of 12 h after the injection. For two more days and during the days before the administration of 17 alpha-OHPC, blood was taken at 8 a.m. before getting up from bed in same intervals, urine was collected for analysis of sodium and potassium excretion. During the first 12 h after the injection of 17 alpha-OHPC, the urinary sodium/potassium ratio significantly increased in all subjects. Plasma renin activity showed no characteristic changes at this time, whereas the plasma concentrations of aldosterone and cortisol decreased. The decrease of cortisol concentration started immediately after the injection and was more pronounced than that of plasma aldosterone. During the following 36 h, renin activity as well as aldosterone and cortisol concentrations in plasma showed an increase; in contrast, the sodium/potassium ratio decreased. On the basis of these results, the following effects of 17 alpha-OHPC are discussed: (1) an acute natriuresis which may be due to a competitive inhibition of aldosterone at the renal tubules, and (2) an inhibition of pituitary ACTH secretion or of adrenal steroid biosynthesis.", "contents": "[Effect of 17-alpha-hydroxyprogesteronecapronate on sodium excretion and the renin-angiotensin-aldosterone-system in humans]. In seven healthy male subjects, a natriuretic effect of 17 alpha-hydroxyprogesterone caproate (17 alpha-OHPC) was demonstrated. Three of these subjects were kept on an uncontrolled diet and were examined over a period of 12 days. To the remaining four subjects, a single dose of 250 mg 17 alpha-OHPC was given intramuscularly after four days of intake of a chemically defined diet (Vivasorb). In this second test procedure, blood samples were taken in the recumbent position every two hours throughout a period of 12 h after the injection. For two more days and during the days before the administration of 17 alpha-OHPC, blood was taken at 8 a.m. before getting up from bed in same intervals, urine was collected for analysis of sodium and potassium excretion. During the first 12 h after the injection of 17 alpha-OHPC, the urinary sodium/potassium ratio significantly increased in all subjects. Plasma renin activity showed no characteristic changes at this time, whereas the plasma concentrations of aldosterone and cortisol decreased. The decrease of cortisol concentration started immediately after the injection and was more pronounced than that of plasma aldosterone. During the following 36 h, renin activity as well as aldosterone and cortisol concentrations in plasma showed an increase; in contrast, the sodium/potassium ratio decreased. On the basis of these results, the following effects of 17 alpha-OHPC are discussed: (1) an acute natriuresis which may be due to a competitive inhibition of aldosterone at the renal tubules, and (2) an inhibition of pituitary ACTH secretion or of adrenal steroid biosynthesis."}
{"id": "PMID:467360", "title": "Seasonal fluctuation in lipid and cholesterol content of ovary, liver and blood serum in relation to annual sexual cycle in Heteropneustes fossilis (Bloch).", "content": "Seasonal fluctuation in lipid content of ovary and liver and cholesterol content of ovary, liver and serum in relation to annual reproductive cycle in H. fossilis has been worked out. Reduction in lipid content of liver was associated with decrease in hepatosomatic index (HSI) in prespawning and spawning phases. But there was an obvious increase in the lipid level of ovary in the above phases which coincided with enhanced gonadosomatic index (GSI) records. Serum and ovary showed a subsidence in their cholesterol contents in prespawning followed by increased level during spawning period. Substraction in liver cholesterol started in prespawning and continued till spawning phase and thereafter regained upward trend. Lipid and cholesterol levels in all organs studied along with GSI and HSI returned to initial values by the end of postspawning stage. Administration of clomid, sexovid, PGE1 and PGF2 alpha for a week did not induce any change in HSI, GSI, lipid and cholesterol values of liver and serum. However, these drugs were effective in suppressing the ovarian cholesterol within a week. The above drugs seem to affect cholesterol level indirectly since they trigger gonadotrophin secretion which in turn probably accelerated cholesterol utilization in ovary for increased steroid hormone output.", "contents": "Seasonal fluctuation in lipid and cholesterol content of ovary, liver and blood serum in relation to annual sexual cycle in Heteropneustes fossilis (Bloch). Seasonal fluctuation in lipid content of ovary and liver and cholesterol content of ovary, liver and serum in relation to annual reproductive cycle in H. fossilis has been worked out. Reduction in lipid content of liver was associated with decrease in hepatosomatic index (HSI) in prespawning and spawning phases. But there was an obvious increase in the lipid level of ovary in the above phases which coincided with enhanced gonadosomatic index (GSI) records. Serum and ovary showed a subsidence in their cholesterol contents in prespawning followed by increased level during spawning period. Substraction in liver cholesterol started in prespawning and continued till spawning phase and thereafter regained upward trend. Lipid and cholesterol levels in all organs studied along with GSI and HSI returned to initial values by the end of postspawning stage. Administration of clomid, sexovid, PGE1 and PGF2 alpha for a week did not induce any change in HSI, GSI, lipid and cholesterol values of liver and serum. However, these drugs were effective in suppressing the ovarian cholesterol within a week. The above drugs seem to affect cholesterol level indirectly since they trigger gonadotrophin secretion which in turn probably accelerated cholesterol utilization in ovary for increased steroid hormone output."}
{"id": "PMID:467361", "title": "Influence of histamine on estrogen-induced inhibition of compensatory ovarian hypertrophy in hemiovariectomized rats.", "content": "The administration of histamine dihydrochloride (5 micrograms) into the lateral brain ventricle, H1-agonist IEM-813 (0.25 mg/kg) intraperitoneally, tavegil (0.05 mg/kg) intraperitoneally and dimedrol (50 mg/kg) subcutaneously did not change the rate of compensatory ovarian hypertrophy (COH) in hemiovariectomized rats. The treatment of rats with histamine or IEM-813 prevented the suppressive action of diethystilbestrol on COH, tavegil did not change and dimedrol enhanced the estrogen effect. Central histaminergic structures are suggested not be involved in the mechanism of PSH secretion. However, their stimulation inhibits the realisation of the negative feedback of estrogen.", "contents": "Influence of histamine on estrogen-induced inhibition of compensatory ovarian hypertrophy in hemiovariectomized rats. The administration of histamine dihydrochloride (5 micrograms) into the lateral brain ventricle, H1-agonist IEM-813 (0.25 mg/kg) intraperitoneally, tavegil (0.05 mg/kg) intraperitoneally and dimedrol (50 mg/kg) subcutaneously did not change the rate of compensatory ovarian hypertrophy (COH) in hemiovariectomized rats. The treatment of rats with histamine or IEM-813 prevented the suppressive action of diethystilbestrol on COH, tavegil did not change and dimedrol enhanced the estrogen effect. Central histaminergic structures are suggested not be involved in the mechanism of PSH secretion. However, their stimulation inhibits the realisation of the negative feedback of estrogen."}
{"id": "PMID:467362", "title": "Reactivity to estradiol of thymic cells from female mice before and after puberty.", "content": "The reactivity to estradiol of thymic cells from female mice representing different stages of sexual maturation was investigated. It was found that estradiol administered in pharmacological doses can evoke in thymuses of ovariectomized mice changes imitating those observed during puberty with the reactivity to estradiol changing along with the process of sexual maturation. It is most likely that the changes of reactivity are due to extragonadal factors.", "contents": "Reactivity to estradiol of thymic cells from female mice before and after puberty. The reactivity to estradiol of thymic cells from female mice representing different stages of sexual maturation was investigated. It was found that estradiol administered in pharmacological doses can evoke in thymuses of ovariectomized mice changes imitating those observed during puberty with the reactivity to estradiol changing along with the process of sexual maturation. It is most likely that the changes of reactivity are due to extragonadal factors."}
{"id": "PMID:467363", "title": "Studies on the effects of ethinyl estradiol and norethisterone acetate on the adrenal cortex and some other tissues in the rat.", "content": "Alkaline phosphatase activity (AP) of the adrenal cortex of rats were determined under the effect of ethinyl estradiol (EE) and/or norethisterone acetate (NA), the two components of the contraceptive pill gyn-anovlar (Schering AG Berlin). A pathological study was also carried out to examine the effects of EE and NA on other tissues mainly the liver, lungs, spleen and ovaries. EE in a dose of 10 micrograms/day for 2 weeks caused a significant increase in the weight of the adrenal but no significant increase in the AP/g cortical tissue. The 25 and 50 micrograms doses for the same period caused a significant increase in both adrenal weight and AP. When treatment was prolonged to 6 weeks no effect on adrenal weight or AP was detected. The same finding was obtained with NA in a dose of 7 mg/rat/day for 2 weeks. The 14 mg dose of NA for the same period caused a significant increase in adrenal weight but no effect on AP. The 21 mg dose caused a significant increase in adrenal weight accompanied by significant decrease in AP/g cortical tissue. Treatment with NA for 6 weeks caused a rise in adrenal weight and AP with the 7 mg dose, then a decline in AP with the 14 mg dose, and a decline in both adrenal weight and AP with the 21 mg dose. As regards the effects of EE and NA on other tissues, EE was found to have a powerful stimulatory effect on the reticuloendothelial system (RES) as well as toxic effects on the liver. NA did not produce such lesions except for the large doses and prolonged periods of treatment. In addition NA produced cholestasis in the liver associated with staining of the liver cells with bile. Combination of EE and NA in the form of gyn-anovlar produced more powerful stimulation of RES and decreased the toxic manifestations of either component. As regards the ovaries, administration of 50 micrograms EE for 6 weeks produced profound hyperplasia of the granulosa cells of the Graafian follicles and inhibited ovulation, however, NA did not inhibit ovulation. With gyn-anovlar, the effect of EE on the ovaries seemed to predominate and ovulation was inhibited.", "contents": "Studies on the effects of ethinyl estradiol and norethisterone acetate on the adrenal cortex and some other tissues in the rat. Alkaline phosphatase activity (AP) of the adrenal cortex of rats were determined under the effect of ethinyl estradiol (EE) and/or norethisterone acetate (NA), the two components of the contraceptive pill gyn-anovlar (Schering AG Berlin). A pathological study was also carried out to examine the effects of EE and NA on other tissues mainly the liver, lungs, spleen and ovaries. EE in a dose of 10 micrograms/day for 2 weeks caused a significant increase in the weight of the adrenal but no significant increase in the AP/g cortical tissue. The 25 and 50 micrograms doses for the same period caused a significant increase in both adrenal weight and AP. When treatment was prolonged to 6 weeks no effect on adrenal weight or AP was detected. The same finding was obtained with NA in a dose of 7 mg/rat/day for 2 weeks. The 14 mg dose of NA for the same period caused a significant increase in adrenal weight but no effect on AP. The 21 mg dose caused a significant increase in adrenal weight accompanied by significant decrease in AP/g cortical tissue. Treatment with NA for 6 weeks caused a rise in adrenal weight and AP with the 7 mg dose, then a decline in AP with the 14 mg dose, and a decline in both adrenal weight and AP with the 21 mg dose. As regards the effects of EE and NA on other tissues, EE was found to have a powerful stimulatory effect on the reticuloendothelial system (RES) as well as toxic effects on the liver. NA did not produce such lesions except for the large doses and prolonged periods of treatment. In addition NA produced cholestasis in the liver associated with staining of the liver cells with bile. Combination of EE and NA in the form of gyn-anovlar produced more powerful stimulation of RES and decreased the toxic manifestations of either component. As regards the ovaries, administration of 50 micrograms EE for 6 weeks produced profound hyperplasia of the granulosa cells of the Graafian follicles and inhibited ovulation, however, NA did not inhibit ovulation. With gyn-anovlar, the effect of EE on the ovaries seemed to predominate and ovulation was inhibited."}
{"id": "PMID:467364", "title": "Indications for a brain uptake of labelled vasopressin and ocytocin and the problem of the blood-brain barrier.", "content": "Fifteen seconds after intracarotid injection of [125J]-lysine vasopressin, [3H]-ocytocin, tritiated water or [3H]-inulin, the distribution of radioactivity in 18 regions of the rat brain and in the anterior pituitary was determined. Comparing the concentration of the different tracers (in % of injected radioactivity per g brain tissue), it is assumed that a small amount of the labelled neurohormones is taken up by the brain, indicating a penetration of the blood-brain barrier and/or an accumulation within the structures of the barrier system.", "contents": "Indications for a brain uptake of labelled vasopressin and ocytocin and the problem of the blood-brain barrier. Fifteen seconds after intracarotid injection of [125J]-lysine vasopressin, [3H]-ocytocin, tritiated water or [3H]-inulin, the distribution of radioactivity in 18 regions of the rat brain and in the anterior pituitary was determined. Comparing the concentration of the different tracers (in % of injected radioactivity per g brain tissue), it is assumed that a small amount of the labelled neurohormones is taken up by the brain, indicating a penetration of the blood-brain barrier and/or an accumulation within the structures of the barrier system."}
{"id": "PMID:467365", "title": "Free fatty acid release from human adipose tissue in relation to obesity -- effect of isoprenaline.", "content": "The capacity to release non-esterified acids and glycerol from the abdominal subcutaneous adipose tissue into a medium containing different concentrations of isoprenaline was studied in 21 adults with different body weight (14 obese, 7 controls). The obese had a significantly lower maximum adipokinetic capacity (expressed as pD2) than controls. The significant decrease in pD2 in the obese was found to depend on the stabilization of the weight while the dynamic pD2 stage values of the obese did not differ from those of controls. The hypothesis is expressed that lower pD2 values may be related to the smaller decrease of body fat during weight reduction.", "contents": "Free fatty acid release from human adipose tissue in relation to obesity -- effect of isoprenaline. The capacity to release non-esterified acids and glycerol from the abdominal subcutaneous adipose tissue into a medium containing different concentrations of isoprenaline was studied in 21 adults with different body weight (14 obese, 7 controls). The obese had a significantly lower maximum adipokinetic capacity (expressed as pD2) than controls. The significant decrease in pD2 in the obese was found to depend on the stabilization of the weight while the dynamic pD2 stage values of the obese did not differ from those of controls. The hypothesis is expressed that lower pD2 values may be related to the smaller decrease of body fat during weight reduction."}
{"id": "PMID:467366", "title": "Radioimmunoassay of porcine growth hormone -- methodological studies.", "content": "A double antibody radioimmunoassay for immunoreactive growth hormone in porcine plasma has been evaluated on the basis of dilution and recovery experiments as well as by examination of accuracy and reproducibility. The sensitivity was better than 0.10 ng/ml. The cumulated within-assay coefficient of variation was between 2--4% and the cumulated between-assay coefficient of variation was 9.6%. The normal fasting values correspond well to those of other investigators.", "contents": "Radioimmunoassay of porcine growth hormone -- methodological studies. A double antibody radioimmunoassay for immunoreactive growth hormone in porcine plasma has been evaluated on the basis of dilution and recovery experiments as well as by examination of accuracy and reproducibility. The sensitivity was better than 0.10 ng/ml. The cumulated within-assay coefficient of variation was between 2--4% and the cumulated between-assay coefficient of variation was 9.6%. The normal fasting values correspond well to those of other investigators."}
{"id": "PMID:467367", "title": "Inhibition of the 3 beta-hydroxysteroid oxidoreductase of Pseudomonas testosteroni by steroids.", "content": "55 Steroids of the estratriene and androstane type with substituents in pos. 16 alpha, 17 alpha or 17 beta were tested for inhibition of the 3beta-hydroxysteroid oxidoreductase of Pseudomonas testosteroni. Estratrien-3-ols were strong and competitive inhibitors (Ki less than 1 micron). Substituents in pos. 16 alpha of estradiol influenced the inhibitory activity distinctly. Substituents in 17 alpha- or 17 beta-position were of slight influence. 3-Methoxy estratrienes gave no inhibition of the enzymic 3 beta-OH-dehydrogenation. The 4-unsaturated 3-oxo-steroids tested were moderate inhibitors (Ki 2.4-70 micron). The activity was slightly influenced by 17 alpha-substituents. It was increased by 10 beta-substituents in the order H less than CH3 less than N3. The inhibition test can be used to select and eliminate very strong synthetic inhibitors, which are known to disturb the metabolism of steroid hormones.", "contents": "Inhibition of the 3 beta-hydroxysteroid oxidoreductase of Pseudomonas testosteroni by steroids. 55 Steroids of the estratriene and androstane type with substituents in pos. 16 alpha, 17 alpha or 17 beta were tested for inhibition of the 3beta-hydroxysteroid oxidoreductase of Pseudomonas testosteroni. Estratrien-3-ols were strong and competitive inhibitors (Ki less than 1 micron). Substituents in pos. 16 alpha of estradiol influenced the inhibitory activity distinctly. Substituents in 17 alpha- or 17 beta-position were of slight influence. 3-Methoxy estratrienes gave no inhibition of the enzymic 3 beta-OH-dehydrogenation. The 4-unsaturated 3-oxo-steroids tested were moderate inhibitors (Ki 2.4-70 micron). The activity was slightly influenced by 17 alpha-substituents. It was increased by 10 beta-substituents in the order H less than CH3 less than N3. The inhibition test can be used to select and eliminate very strong synthetic inhibitors, which are known to disturb the metabolism of steroid hormones."}
{"id": "PMID:467368", "title": "Effects of methallibure (I.C.I. 33828) on the female reproductive tract of the Indian Desert gerbil (Meriones hurrianae Jerdon).", "content": "Effects of methallibure (I.C.I. 33828) have been studied on the reproductive organs of female gerbils, Meriones hurrianae. Methallibure administration caused reduction of gonadal weight indicating suppression of pituitary gonadotrophins. In the ovaries of the treated females extensive atresia of follicles and of corpora lutea was observed. In addition methallibure interrupted the sex cycle, and a dioestrous vaginal pattern was retained throughout medication. A significant decrease in the level of RNA, protein and sialic acid in the uterus and vagina and of glycogen in the uterus was found. Histological and biochemical changes in the ovaries and uteri suggest a possible direct antioestrogenic action of the compound.", "contents": "Effects of methallibure (I.C.I. 33828) on the female reproductive tract of the Indian Desert gerbil (Meriones hurrianae Jerdon). Effects of methallibure (I.C.I. 33828) have been studied on the reproductive organs of female gerbils, Meriones hurrianae. Methallibure administration caused reduction of gonadal weight indicating suppression of pituitary gonadotrophins. In the ovaries of the treated females extensive atresia of follicles and of corpora lutea was observed. In addition methallibure interrupted the sex cycle, and a dioestrous vaginal pattern was retained throughout medication. A significant decrease in the level of RNA, protein and sialic acid in the uterus and vagina and of glycogen in the uterus was found. Histological and biochemical changes in the ovaries and uteri suggest a possible direct antioestrogenic action of the compound."}
{"id": "PMID:467369", "title": "Urinary androgen- and estrogen excretion in men with pachydermia laryngis and cancer of the larynx.", "content": "Considering the larynx as a hormone dependent secondary sex characteristic has previously led to successful antiandrogentherapy of pachydermia of the vocal cords, which may constitute a precancerous state. As a first step to further evaluate the endocrine state of patients with precancerous lesions or cancer of the larynx, the urinary excretion of 17-hydroxysteroids, 17-ketosteroids, testosterone and estrogens has been determined in male patients with pachydermia laryngis (n = 15) or cancer of the larynx (n = 20) as compared to controls with different other otorhino-laryngological affections (n = 20). No difference between groups was found in 17-hydroxysteroids and no significant difference in 17-ketosteroid excretion. The pachydermia group as a whole showed significantly increased levels of testosterone (p = 0.01) and estrogen (p = 0.04) of 64.6 +/- 39.9 microgram/24 hr testosterone versus 34.7 +/- 19.3 microgram/24 hr in controls and  31.7 +/- 16 microgram/24 hr in laryngeal cancer and 277 +/- 14.8 microgram/24 hr total estrogens versus 19.1 +/- 12 microgram/24 hr and 17.8 +/- 8.1 microgram/24 hr respectively. These data further support the idea of hormonal factors playing an important role in the pathogenesis of pachydermia and thus possibly cancer of the larynx. So far, however, they do not permit definite conclusions on the pathogenetic mechanisms involved.", "contents": "Urinary androgen- and estrogen excretion in men with pachydermia laryngis and cancer of the larynx. Considering the larynx as a hormone dependent secondary sex characteristic has previously led to successful antiandrogentherapy of pachydermia of the vocal cords, which may constitute a precancerous state. As a first step to further evaluate the endocrine state of patients with precancerous lesions or cancer of the larynx, the urinary excretion of 17-hydroxysteroids, 17-ketosteroids, testosterone and estrogens has been determined in male patients with pachydermia laryngis (n = 15) or cancer of the larynx (n = 20) as compared to controls with different other otorhino-laryngological affections (n = 20). No difference between groups was found in 17-hydroxysteroids and no significant difference in 17-ketosteroid excretion. The pachydermia group as a whole showed significantly increased levels of testosterone (p = 0.01) and estrogen (p = 0.04) of 64.6 +/- 39.9 microgram/24 hr testosterone versus 34.7 +/- 19.3 microgram/24 hr in controls and  31.7 +/- 16 microgram/24 hr in laryngeal cancer and 277 +/- 14.8 microgram/24 hr total estrogens versus 19.1 +/- 12 microgram/24 hr and 17.8 +/- 8.1 microgram/24 hr respectively. These data further support the idea of hormonal factors playing an important role in the pathogenesis of pachydermia and thus possibly cancer of the larynx. So far, however, they do not permit definite conclusions on the pathogenetic mechanisms involved."}
{"id": "PMID:467370", "title": "Gonadotrophin determination in 24-h-urine by sensitive LH and FSH radioimmunoassays.", "content": "Urinary Lutropin (LH) and Follitropin (FSH) were precipitated with acetone and measured by specific radioimmunoassays. Recovery experiments with special regard to urinary pH values were done by adding 125I-labelled LH and FSH to unprocessed urine. At pH 4.5 68.6% of LH and 66.1% of FSH were recovered. Increasing pH values up to 6.0 resulted in a significant recovery loss of 125-I-LH, but not of 125I-FSH. Immunoreactive FSH concentrations decreased significantly 6 and 48 hours after storage began at room temperature. In healthy males and females we found mean LH and FSH concentrations in 24-h-urine which were similar to those reported by others: LH: males: 22.6 IU/24-h; cycling females: 17.8 IU/24-h; postmenopausal females: 49.1 IU/24-h; FSH: males 6.0 IU/24-h: cycling females: 15.0 IU/24-h; postmenopausal females: 48.5 IU/24-h. Because of their high sensitivity and practicability, we believe that these radioimmunoassays are clinically useful approaches in assessment of pituitary and gonadal disorders in human subjects.", "contents": "Gonadotrophin determination in 24-h-urine by sensitive LH and FSH radioimmunoassays. Urinary Lutropin (LH) and Follitropin (FSH) were precipitated with acetone and measured by specific radioimmunoassays. Recovery experiments with special regard to urinary pH values were done by adding 125I-labelled LH and FSH to unprocessed urine. At pH 4.5 68.6% of LH and 66.1% of FSH were recovered. Increasing pH values up to 6.0 resulted in a significant recovery loss of 125-I-LH, but not of 125I-FSH. Immunoreactive FSH concentrations decreased significantly 6 and 48 hours after storage began at room temperature. In healthy males and females we found mean LH and FSH concentrations in 24-h-urine which were similar to those reported by others: LH: males: 22.6 IU/24-h; cycling females: 17.8 IU/24-h; postmenopausal females: 49.1 IU/24-h; FSH: males 6.0 IU/24-h: cycling females: 15.0 IU/24-h; postmenopausal females: 48.5 IU/24-h. Because of their high sensitivity and practicability, we believe that these radioimmunoassays are clinically useful approaches in assessment of pituitary and gonadal disorders in human subjects."}
{"id": "PMID:467371", "title": "Study of plasma aldosterone in normal pregnancy, in pre-eclamptic women and in cord plasma of newborns.", "content": "A radioimmunoassay without chromatography was used for the determination of plasma aldosterone in pregnancy. The mean values (+/- S.D.) of aldosterone concentration increased consistently from 23.2 +/- 5.3 ng/100 ml (n = 14) during the first trimester to 37.2 +/- 10.6 ng/100 ml (n = 17) during the second trimester and 64.0 +/- 18.8 ng/100 ml (n = 29) during the third trimester of pregnancy. The highest values were found at delivery (71.9 +/- 14.2 ng/100 ml; n = 21) and in the cord plasma of newborns (83.4 +/- 14.9 ng/100 ml; n = 21). Significantly lower plasma aldosterone values were found in the plasma of pre-eclamptic women during the third trimester of pregnancy (41.9 +/- 21.3 ng/100 ml; n = 11).", "contents": "Study of plasma aldosterone in normal pregnancy, in pre-eclamptic women and in cord plasma of newborns. A radioimmunoassay without chromatography was used for the determination of plasma aldosterone in pregnancy. The mean values (+/- S.D.) of aldosterone concentration increased consistently from 23.2 +/- 5.3 ng/100 ml (n = 14) during the first trimester to 37.2 +/- 10.6 ng/100 ml (n = 17) during the second trimester and 64.0 +/- 18.8 ng/100 ml (n = 29) during the third trimester of pregnancy. The highest values were found at delivery (71.9 +/- 14.2 ng/100 ml; n = 21) and in the cord plasma of newborns (83.4 +/- 14.9 ng/100 ml; n = 21). Significantly lower plasma aldosterone values were found in the plasma of pre-eclamptic women during the third trimester of pregnancy (41.9 +/- 21.3 ng/100 ml; n = 11)."}
{"id": "PMID:467372", "title": "Study on the inactivation mechanism of androgens in rheumatoid arthritis: excretory rate of free and conjugated 17-ketosteroids.", "content": "Unconjugated, sulpho- and glucurono-conjugated androgen hormone metabolites have been determined in the urine of patients with rheumatoid arthritis. An increase in the excretory rate of unconjugated 5 beta-reduced 17-ketosteroids and a decrease in that of 17-ketosteroid conjugates, especially in dehydroepiandrosterone sulphate and in the sum of dehydroepiandrosterone, etiocholanolone and androsterone glucuronoside were observed. In contrast to unconjugated metabolites, there was less significant change in the 5 beta-metabolite conjugates in urine. Corticosteroid treatment resulted in an additional decrease of metabolite excretion by patients. Further study is necessary to determine the causative factors in the altered steroid pattern observed in this severe, non-endocrine disease.", "contents": "Study on the inactivation mechanism of androgens in rheumatoid arthritis: excretory rate of free and conjugated 17-ketosteroids. Unconjugated, sulpho- and glucurono-conjugated androgen hormone metabolites have been determined in the urine of patients with rheumatoid arthritis. An increase in the excretory rate of unconjugated 5 beta-reduced 17-ketosteroids and a decrease in that of 17-ketosteroid conjugates, especially in dehydroepiandrosterone sulphate and in the sum of dehydroepiandrosterone, etiocholanolone and androsterone glucuronoside were observed. In contrast to unconjugated metabolites, there was less significant change in the 5 beta-metabolite conjugates in urine. Corticosteroid treatment resulted in an additional decrease of metabolite excretion by patients. Further study is necessary to determine the causative factors in the altered steroid pattern observed in this severe, non-endocrine disease."}
{"id": "PMID:467373", "title": "Regulations of plasma aldosterone in young hyperkalemic patients with stable chronic renal failure.", "content": "In a group of four young patients with stable chronic renal failure and hyperkalemia sodium restriction induced a remarkable increase in plasma renin activity (PRA) and plasma aldosterone (PA), a decrease in the elevated serum potassium (SK) and a rise in potassium excretion. During high sodium intake the levels of PRA and PA were lower than those found in the healthy control group suggesting that enhanced suppressibility of the renin-angiotensin-aldosterone system (RAAS) was the main cause of hyperkalemia. During sodium restriction despite a marked increase in PRA and PA levels poor correlations were found between these variables indicating disorganisation within the RAAS and probably a diminished role for renin-angiotensin in the regulation of aldosterone production in three hyperkalemic patients with chronic glomerulonephritis. On the other hand, in the same patients significant correlations were found between fluctuations of SK and PA on constant normal and low sodium diets supporting the concept of an (at least) equal role of potassium and RAAS in the acute regulation of PA. A prominent role for SK was found in an unusual hyperkalemic patient with interstitial nephritis when PRA was suppressed and the elevated SK showed a definite postural rise inducing dramatic increases in PA in the upright posture. Reversion of the postural SK rise masked again the governing role of SK.", "contents": "Regulations of plasma aldosterone in young hyperkalemic patients with stable chronic renal failure. In a group of four young patients with stable chronic renal failure and hyperkalemia sodium restriction induced a remarkable increase in plasma renin activity (PRA) and plasma aldosterone (PA), a decrease in the elevated serum potassium (SK) and a rise in potassium excretion. During high sodium intake the levels of PRA and PA were lower than those found in the healthy control group suggesting that enhanced suppressibility of the renin-angiotensin-aldosterone system (RAAS) was the main cause of hyperkalemia. During sodium restriction despite a marked increase in PRA and PA levels poor correlations were found between these variables indicating disorganisation within the RAAS and probably a diminished role for renin-angiotensin in the regulation of aldosterone production in three hyperkalemic patients with chronic glomerulonephritis. On the other hand, in the same patients significant correlations were found between fluctuations of SK and PA on constant normal and low sodium diets supporting the concept of an (at least) equal role of potassium and RAAS in the acute regulation of PA. A prominent role for SK was found in an unusual hyperkalemic patient with interstitial nephritis when PRA was suppressed and the elevated SK showed a definite postural rise inducing dramatic increases in PA in the upright posture. Reversion of the postural SK rise masked again the governing role of SK."}
{"id": "PMID:467374", "title": "Relationship between insulin and somatotropin in obesity.", "content": "The authors have estimated a correlation between the total insulin areas after glucose loading and total somatotropin areas during insulin-induced hypoglycaemia in 30 patients with obesity, as well as in a selected group of 16 patients with simple (essential) obesity. A significant negative correlation was found in both investigated groups. A new hypothesis assuming hyposecretion of somatotropin in obese subjects as a result of hyperinsulinaemia and subsequent increased somatomedin generation is suggested.", "contents": "Relationship between insulin and somatotropin in obesity. The authors have estimated a correlation between the total insulin areas after glucose loading and total somatotropin areas during insulin-induced hypoglycaemia in 30 patients with obesity, as well as in a selected group of 16 patients with simple (essential) obesity. A significant negative correlation was found in both investigated groups. A new hypothesis assuming hyposecretion of somatotropin in obese subjects as a result of hyperinsulinaemia and subsequent increased somatomedin generation is suggested."}
{"id": "PMID:467375", "title": "Basal and stimulated hyperinsulinemia in obesity: relationship to adipose-cell size.", "content": "In 17 non-selected, non-hyperlipoproteinemic subjects without overt diabetes both adipose tissue biopsy and an oral glucose tolerance test (50 g) were performed. All persons were weight-stable at the time of investigation. A significant correlation between fasting insulin concentration and mean adipocyte volume was observed, whereas no correlation existed between ideal body weight index and fasting insulin level. Persons with larger adipocytes had elevated basal insulin levels as well as higher and longer lasting increments following the glucose challenge. They also exhibited significantly higher mean glucose levels during the OGTT. When these patients were matched for glucose tolerance with the subgroup having smaller mean adipocyte volumes, the difference in insulin levels was still demonstrable. This study underlines the importance of adipose-cell enlargement regulating basal and stimulated insulin output.", "contents": "Basal and stimulated hyperinsulinemia in obesity: relationship to adipose-cell size. In 17 non-selected, non-hyperlipoproteinemic subjects without overt diabetes both adipose tissue biopsy and an oral glucose tolerance test (50 g) were performed. All persons were weight-stable at the time of investigation. A significant correlation between fasting insulin concentration and mean adipocyte volume was observed, whereas no correlation existed between ideal body weight index and fasting insulin level. Persons with larger adipocytes had elevated basal insulin levels as well as higher and longer lasting increments following the glucose challenge. They also exhibited significantly higher mean glucose levels during the OGTT. When these patients were matched for glucose tolerance with the subgroup having smaller mean adipocyte volumes, the difference in insulin levels was still demonstrable. This study underlines the importance of adipose-cell enlargement regulating basal and stimulated insulin output."}
{"id": "PMID:467378", "title": "Changes in plasma oestradiol-17beta, progesterone, testosterone, LH and fertility after treatment with I.C.I. 80, 996.", "content": "Fifty heifers were twice injected with I.C.I. 80, 996 and inseminated 72 h and 96 h after the second administration. Twenty eight of them (56%) became pregnant. Changes in plasma oestradiol-17 beta, progesterone and LH concentrations around the oestrus following the second injection were similar to those occurring in spontaneous oestrus. The pattern of testosterone secretion resembled that of oestradiol;-17 beta. The highest testosterone concentration (135 +/- 24 pg/ml) was measured on the third day after treatment with I.C.I. 80, 996.", "contents": "Changes in plasma oestradiol-17beta, progesterone, testosterone, LH and fertility after treatment with I.C.I. 80, 996. Fifty heifers were twice injected with I.C.I. 80, 996 and inseminated 72 h and 96 h after the second administration. Twenty eight of them (56%) became pregnant. Changes in plasma oestradiol-17 beta, progesterone and LH concentrations around the oestrus following the second injection were similar to those occurring in spontaneous oestrus. The pattern of testosterone secretion resembled that of oestradiol;-17 beta. The highest testosterone concentration (135 +/- 24 pg/ml) was measured on the third day after treatment with I.C.I. 80, 996."}
{"id": "PMID:467379", "title": "Studies on the erythropoietic effect of plasma from anemic toads both with and without testis.", "content": "Injection of plasma from experimentally induced anemic toad with intact testis increases erythropoiesis in starved toads evidenced by the increase of red blood cell, hemoglobin and hematocrit, whereas the plasma of castrated and phenylhydrazine-HCl treated anemic toad failed to do so. It can be suggested that the erythropoiesis stimulating factor (ESF) is produced from the testis of toad and the production of this factor (ESF) was found to increase during anemia in an attempt to correct the anemic condition.", "contents": "Studies on the erythropoietic effect of plasma from anemic toads both with and without testis. Injection of plasma from experimentally induced anemic toad with intact testis increases erythropoiesis in starved toads evidenced by the increase of red blood cell, hemoglobin and hematocrit, whereas the plasma of castrated and phenylhydrazine-HCl treated anemic toad failed to do so. It can be suggested that the erythropoiesis stimulating factor (ESF) is produced from the testis of toad and the production of this factor (ESF) was found to increase during anemia in an attempt to correct the anemic condition."}
{"id": "PMID:467395", "title": "A comparison of two proxy measures for morbidity.", "content": "The use of standardised mortality ratios (SMRs) as a proxy measure of morbidity for resource allocation purposes in the National Health Service (NHS) has been widely criticised. The small number of deaths from infectious diseases makes it necessary to consider whether there are more appropriate measures. The standardised notification rate (SNR) was compared with the SMR for respiratory tuberculosis and it appears to be a better proxy for morbidity.", "contents": "A comparison of two proxy measures for morbidity. The use of standardised mortality ratios (SMRs) as a proxy measure of morbidity for resource allocation purposes in the National Health Service (NHS) has been widely criticised. The small number of deaths from infectious diseases makes it necessary to consider whether there are more appropriate measures. The standardised notification rate (SNR) was compared with the SMR for respiratory tuberculosis and it appears to be a better proxy for morbidity."}
{"id": "PMID:467396", "title": "Some insights into Miettinen's multivariate confounder score approach to case-control study analysis.", "content": "We have studied Miettinen's multivariate confounder score method of controlling confounding in case-control studies both theoretically and by simulation. The main conclusion to be drawn from out results is that the method will in many practical situations seriously exaggerate the statistical significance achieved, and its use is not to be recommended.", "contents": "Some insights into Miettinen's multivariate confounder score approach to case-control study analysis. We have studied Miettinen's multivariate confounder score method of controlling confounding in case-control studies both theoretically and by simulation. The main conclusion to be drawn from out results is that the method will in many practical situations seriously exaggerate the statistical significance achieved, and its use is not to be recommended."}
{"id": "PMID:467400", "title": "The sex differential in ischaemic heart disease: trends by social class 1931 to 1971.", "content": "The comparison of trends in ischaemic heart disease (IHD) mortality in different social classes is confounded by historical changes in diagnostic techniques and statistical classification, and possibly by different standards of diagnostic accuracy in the different social classes. The problems can be circumvented by taking advantage of the fact that in middle age (45 to 64) the IHD death rate is much higher in men than in women. This large sex differential is not present in any of the other causes of death with which IHD might easily be confused and it is therefore relatively unaffected by diagnostic errors and variation in classification. The changes that have occurred in the sex differential in Social Classes I and V in England and Wales between 1931 and 1971 confirm anecdotal clinical reports that the male vulnerability to IHD appeared first in Social Class I (professionsl). By 1971 Social Class V (unskilled) had caught up, and the men in these two social classes now experience an almost identical excess in cardiovascular death rate compared with their wives.", "contents": "The sex differential in ischaemic heart disease: trends by social class 1931 to 1971. The comparison of trends in ischaemic heart disease (IHD) mortality in different social classes is confounded by historical changes in diagnostic techniques and statistical classification, and possibly by different standards of diagnostic accuracy in the different social classes. The problems can be circumvented by taking advantage of the fact that in middle age (45 to 64) the IHD death rate is much higher in men than in women. This large sex differential is not present in any of the other causes of death with which IHD might easily be confused and it is therefore relatively unaffected by diagnostic errors and variation in classification. The changes that have occurred in the sex differential in Social Classes I and V in England and Wales between 1931 and 1971 confirm anecdotal clinical reports that the male vulnerability to IHD appeared first in Social Class I (professionsl). By 1971 Social Class V (unskilled) had caught up, and the men in these two social classes now experience an almost identical excess in cardiovascular death rate compared with their wives."}
{"id": "PMID:467402", "title": "The influence of a premarital conception and various obstetric complications on subsequent prenatal health behaviour.", "content": "This paper is a companion to an earlier report on prenatal visiting patterns in Aberdeen, Scotland (McKinlay, 1970). It examines the following three main questions: (1) Is the emerging trend towards later maternity care among young nulliparae largely due to those who delay because of premarital conception? (2) If premarital conception is primarily responsible for this trend, is this pattern of visiting behaviour continued in subsequent pregnancies, and what variation is there in such behaviour--between, for example, different socioeconomic groups and age groups? (3) Does the presence of one or more obstetric complications associated with a first pregnancy or birth have an effect on subsequent prenatal health behaviour, and does this effect, if present, interact with, say, socioeconomic status, or the legal status of the first pregnancy? The paper concludes with a discussion of some policy implications of the findings.", "contents": "The influence of a premarital conception and various obstetric complications on subsequent prenatal health behaviour. This paper is a companion to an earlier report on prenatal visiting patterns in Aberdeen, Scotland (McKinlay, 1970). It examines the following three main questions: (1) Is the emerging trend towards later maternity care among young nulliparae largely due to those who delay because of premarital conception? (2) If premarital conception is primarily responsible for this trend, is this pattern of visiting behaviour continued in subsequent pregnancies, and what variation is there in such behaviour--between, for example, different socioeconomic groups and age groups? (3) Does the presence of one or more obstetric complications associated with a first pregnancy or birth have an effect on subsequent prenatal health behaviour, and does this effect, if present, interact with, say, socioeconomic status, or the legal status of the first pregnancy? The paper concludes with a discussion of some policy implications of the findings."}
{"id": "PMID:467403", "title": "Distance as an influence on demand in general practice.", "content": "A survey of general practice in the London borough of Lambeth was analysed to discover how the distance from a patient's home to a medical facility affects the utilisation of that facility. It was shown that distance has a negative effect on utilisation for all population groups except men aged 15 to 64. The effect was especially marked for women, for the elderly, and for Social Classes III, IV, and V, which are the groups with highest utilisation rates. Some diagnostic groups singled out for analysis showed the same effect. These results are related to other studies and to an economic theory seeking to explain patterns of demand.", "contents": "Distance as an influence on demand in general practice. A survey of general practice in the London borough of Lambeth was analysed to discover how the distance from a patient's home to a medical facility affects the utilisation of that facility. It was shown that distance has a negative effect on utilisation for all population groups except men aged 15 to 64. The effect was especially marked for women, for the elderly, and for Social Classes III, IV, and V, which are the groups with highest utilisation rates. Some diagnostic groups singled out for analysis showed the same effect. These results are related to other studies and to an economic theory seeking to explain patterns of demand."}
{"id": "PMID:467410", "title": "Metabolic adaptation to prolonged exercise.", "content": "A study was undertaken to evaluate and to examine the role of substrate supply in 50 healthy subjects after long distance events, such as 10 km, 25 km, and marathon races. The metabolic, variables of carbohydrate metabolism were greatest in 10-km runners, with the highest increase in glucose, lactate, and pyruvate, while in marathon runners only moderate changes were observed. Marathon competitors gave the greatest decrease in insulin concentration whereas glucagon and cortisol showed a contrary tendency. As for lipid concentrations, the most remarkable point was that after the marathon competition the best runners had the highest increase in free fatty acids; the longer the race, the higher were the beta-hydroxybutyrate and acetoacetate levels after the competition. It is important to emphasize that the limiting factor up to 90 min duration is the competitor's ability to deplete the stores of glycogen. Beyond 90 min (or 25 km) the decrease in insulin, the rise in cortisol and the higher concentration of ketnne bodies found indicate a change in metabnlic response.", "contents": "Metabolic adaptation to prolonged exercise. A study was undertaken to evaluate and to examine the role of substrate supply in 50 healthy subjects after long distance events, such as 10 km, 25 km, and marathon races. The metabolic, variables of carbohydrate metabolism were greatest in 10-km runners, with the highest increase in glucose, lactate, and pyruvate, while in marathon runners only moderate changes were observed. Marathon competitors gave the greatest decrease in insulin concentration whereas glucagon and cortisol showed a contrary tendency. As for lipid concentrations, the most remarkable point was that after the marathon competition the best runners had the highest increase in free fatty acids; the longer the race, the higher were the beta-hydroxybutyrate and acetoacetate levels after the competition. It is important to emphasize that the limiting factor up to 90 min duration is the competitor's ability to deplete the stores of glycogen. Beyond 90 min (or 25 km) the decrease in insulin, the rise in cortisol and the higher concentration of ketnne bodies found indicate a change in metabnlic response."}
{"id": "PMID:467411", "title": "Factors influencing quantified surface EMGs.", "content": "Isometric flexions of the elbow are studied in man. For the three joint angles studied, the torque, the surface EMG of the biceps brachii muscle, and the quantified EMG are recorded. The EMGs are picked up by means of bipolar electrodes located in such a way as to vary the interelectrode orientation, the interelectrode distance and the position on the muscle. Longitudinal placements collect stronger signals that transverse ones. Under certain conditions, the magnitude of the quantified EMG depends on the distance between the electrodes, and on their location on the muscle. The results are discussed in terms of conduction volume and detection volume.", "contents": "Factors influencing quantified surface EMGs. Isometric flexions of the elbow are studied in man. For the three joint angles studied, the torque, the surface EMG of the biceps brachii muscle, and the quantified EMG are recorded. The EMGs are picked up by means of bipolar electrodes located in such a way as to vary the interelectrode orientation, the interelectrode distance and the position on the muscle. Longitudinal placements collect stronger signals that transverse ones. Under certain conditions, the magnitude of the quantified EMG depends on the distance between the electrodes, and on their location on the muscle. The results are discussed in terms of conduction volume and detection volume."}
{"id": "PMID:467412", "title": "Intra- and extravascular volume changes in the human forearm after static hand grip exercise.", "content": "Studies have been conducted to evaluate intra- and extravascular volume changes and blood flow in the exercising human forearm by means of (1) combining plethysmographic and scintigraphic methods, (2) an indirect procedure using the relationship of blood flow and volume change from reactive hyperemia. A static hand grip exercise of 60% maximal voluntary contraction and 30 s duration increased the forearm volume by 3.03 +/- 0.65 ml/100 ml soft tissue, involving both the intra- and extravascular volume components. There is a quantitative and qualitative difference in the time course of change in these components, showing an extravascular part of about 50% for the 2-min post-exercise value and a substantially slower rate of recovery. Experiments involving muscle work at intervals (50% maximal voluntary contraction, 30 s duration, 2-min intervals) caused a further increase in extravascular volume. Furthermore, the study suggests that the flow-volume relationship from reactive hyperemia may be considered to be available for the determination of local blood volume changes in exercise hyperemia. The results are discussed in connection with the influence of anaerobic muscle metabolism and conclusions referring to this are drawn about the use of plethysmographic methods.", "contents": "Intra- and extravascular volume changes in the human forearm after static hand grip exercise. Studies have been conducted to evaluate intra- and extravascular volume changes and blood flow in the exercising human forearm by means of (1) combining plethysmographic and scintigraphic methods, (2) an indirect procedure using the relationship of blood flow and volume change from reactive hyperemia. A static hand grip exercise of 60% maximal voluntary contraction and 30 s duration increased the forearm volume by 3.03 +/- 0.65 ml/100 ml soft tissue, involving both the intra- and extravascular volume components. There is a quantitative and qualitative difference in the time course of change in these components, showing an extravascular part of about 50% for the 2-min post-exercise value and a substantially slower rate of recovery. Experiments involving muscle work at intervals (50% maximal voluntary contraction, 30 s duration, 2-min intervals) caused a further increase in extravascular volume. Furthermore, the study suggests that the flow-volume relationship from reactive hyperemia may be considered to be available for the determination of local blood volume changes in exercise hyperemia. The results are discussed in connection with the influence of anaerobic muscle metabolism and conclusions referring to this are drawn about the use of plethysmographic methods."}
{"id": "PMID:467413", "title": "The perception of effort: an inductive approach.", "content": "A theoretical model has been proposed relating physical effort to perceived exertion. The model has been applied to a comparative investigation of the perception of various forces exerted by the adductor pollicis muscle and the quadriceps in five male subjects. The increase in perception of effort with increasing applied force in both muscle groups has been shown to increase exponentially. A force constant, defined in the paper, as the applied force at which perception of effort approximates two-thirds of maximum seems to discriminate effectively between the muscle groups concerned. The description of psychophysical data in this concise quantifiable manner may offer better insight into physiological processes contributing to the appreciation of effort.", "contents": "The perception of effort: an inductive approach. A theoretical model has been proposed relating physical effort to perceived exertion. The model has been applied to a comparative investigation of the perception of various forces exerted by the adductor pollicis muscle and the quadriceps in five male subjects. The increase in perception of effort with increasing applied force in both muscle groups has been shown to increase exponentially. A force constant, defined in the paper, as the applied force at which perception of effort approximates two-thirds of maximum seems to discriminate effectively between the muscle groups concerned. The description of psychophysical data in this concise quantifiable manner may offer better insight into physiological processes contributing to the appreciation of effort."}
{"id": "PMID:467414", "title": "Serial isometric contractions under imposed myotatic stretch conditions in high-strength and low-strength men.", "content": "The immediate effects of an imposed myotatic stretch on knee extensor force were studied in 12 high-strength and 12 low-strength men. Under nonfatigued pre-exercise conditions, significant tension increases of 6.5% for the high-strength group and 11.0% for low-strength subjects were observed as a result of the imposed stretch. An exercise treatment involving 28 serial isometic contractions, each of 5 s duration, with an intertrial rest period of 10 s was administered. This fatiguing exercise resulted in significant decrements in strength on the order of 28.0% and 18.5% for the high-strength and low-strength groups, respectively. A Similar treatment which included a 1 s imposed myotatic stretch during each trial resulted in a greater strength decrement for the low-strength group (26.4%) than for the high-strength subjects (15.0%). A neural factor involving the stretch reflex is tentatively suggested as a plausible explanation accounting for the observation that high-strength subjects fatigue faster than low-strength subjects under conditions of isometric exercise, while low-strength subjects fatigue faster than high-strength individuals in isometric exercise which is performed with an imposed stretch.", "contents": "Serial isometric contractions under imposed myotatic stretch conditions in high-strength and low-strength men. The immediate effects of an imposed myotatic stretch on knee extensor force were studied in 12 high-strength and 12 low-strength men. Under nonfatigued pre-exercise conditions, significant tension increases of 6.5% for the high-strength group and 11.0% for low-strength subjects were observed as a result of the imposed stretch. An exercise treatment involving 28 serial isometic contractions, each of 5 s duration, with an intertrial rest period of 10 s was administered. This fatiguing exercise resulted in significant decrements in strength on the order of 28.0% and 18.5% for the high-strength and low-strength groups, respectively. A Similar treatment which included a 1 s imposed myotatic stretch during each trial resulted in a greater strength decrement for the low-strength group (26.4%) than for the high-strength subjects (15.0%). A neural factor involving the stretch reflex is tentatively suggested as a plausible explanation accounting for the observation that high-strength subjects fatigue faster than low-strength subjects under conditions of isometric exercise, while low-strength subjects fatigue faster than high-strength individuals in isometric exercise which is performed with an imposed stretch."}
{"id": "PMID:467415", "title": "Influence of running pace upon performance: effects upon treadmill endurance time and oxygen cost.", "content": "Three possible patterns of pacing (type 1, fast/slow; type 2, slow/fast; and type 3, steady rate) were compared over a 1400 m, 4 min run. The subsequent running time to exhaustion at 370 m . min-1 was significantly longer with a type 1 than with a type 2 protocol (P less than 0.05). The steady rate pattern gave results intermediate between type 1 and type 2 pacing. Data for oxygen debt and recovery heart rate confirmed the superiority of type 1 pacing. Possible explanations included (1) a reduction of inefficient anaerobic work, and (2) a greater mechanical efficiency associated with the better matching of required effort to a tapering physiological power. Runners should aim at a steady physiological rather than a steady physical load.", "contents": "Influence of running pace upon performance: effects upon treadmill endurance time and oxygen cost. Three possible patterns of pacing (type 1, fast/slow; type 2, slow/fast; and type 3, steady rate) were compared over a 1400 m, 4 min run. The subsequent running time to exhaustion at 370 m . min-1 was significantly longer with a type 1 than with a type 2 protocol (P less than 0.05). The steady rate pattern gave results intermediate between type 1 and type 2 pacing. Data for oxygen debt and recovery heart rate confirmed the superiority of type 1 pacing. Possible explanations included (1) a reduction of inefficient anaerobic work, and (2) a greater mechanical efficiency associated with the better matching of required effort to a tapering physiological power. Runners should aim at a steady physiological rather than a steady physical load."}
{"id": "PMID:467416", "title": "Post-competition blood lactate concentrations in collegiate swimmers.", "content": "The purpose of this investigation was to quantitate post-competition lactate (LA) concentrations of swimmers during a competitive collegiate meet. Blood LA was measured by an enzymatic method on 23 subjects 5 min after each race event. The largest mean LA concentration of 25.7 mM/L was observed in swimmers after competing in the 200-yd individual medley. Swimmers in the 200-yd butterfly, back, breast and freestyle races had similar mean blood LA concentrations (ranging from 16.4 to 20.6 mM/L). Swimmers in the two longest events, the 500-yd and 1,000-yd free style races, had mean LA concentrations of 15.6 and 10.0 mM/L, respectively. To account for the effects of motivation, LA concentrations were measured following maximal effort noncompetitive 100 and 200-yd swims. LA concentrations were slightly greater in conjunction with faster performances for the competitive as compared to the noncompetitive 100 and 200-yd swims.", "contents": "Post-competition blood lactate concentrations in collegiate swimmers. The purpose of this investigation was to quantitate post-competition lactate (LA) concentrations of swimmers during a competitive collegiate meet. Blood LA was measured by an enzymatic method on 23 subjects 5 min after each race event. The largest mean LA concentration of 25.7 mM/L was observed in swimmers after competing in the 200-yd individual medley. Swimmers in the 200-yd butterfly, back, breast and freestyle races had similar mean blood LA concentrations (ranging from 16.4 to 20.6 mM/L). Swimmers in the two longest events, the 500-yd and 1,000-yd free style races, had mean LA concentrations of 15.6 and 10.0 mM/L, respectively. To account for the effects of motivation, LA concentrations were measured following maximal effort noncompetitive 100 and 200-yd swims. LA concentrations were slightly greater in conjunction with faster performances for the competitive as compared to the noncompetitive 100 and 200-yd swims."}
{"id": "PMID:467418", "title": "Anticooperative binding of L-tryptophan to tryptophanyl-tRNA synthetase from beef pancreas. Study at equilibrium by dialysis and changes in spectroscopic properties.", "content": "Equilibrium dialysis and gel filtration studies show that tryptophanyl-tRNA synthetase from beef pancreas binds two molecules of L-tryptophan per dimer in an anticooperative way. The binding of tryptophan ellicits a series of spectroscopic changes in the protein as seen by absorbance, fluorescence and circular dichroism. The molar absorption change of the protein-tryptophan system upon formation of the complex is delta epsilon292 = 10 400 +/- 1000 M(-1) cm(-1) per dimer. Taking an initial symmetrical dimeric protein the two dissociation constants for tryptophan at pH 8, 25 degrees C are respectively K1 = 2.0 +/- 0.5 muM and K2 = 10 +/- 4 muM. They are respectively K1 = 1 +/- 0.25 muM and K2 = 20 +/- 8 muM if one considers a sequenced binding of the two tryptophan molecules. The dichroic band at 290 nm of the free protein disappears when tryptophan is bound. All observed changes are characteristic of tryptophan perturbation and none of tyrosine perturbation. They all exceed the effect that can be expected from the change in environment of the bound tryptophan molecules and modifications of the tertiary structure of the protein have to be taken into account to explain the observed spectroscopic data.", "contents": "Anticooperative binding of L-tryptophan to tryptophanyl-tRNA synthetase from beef pancreas. Study at equilibrium by dialysis and changes in spectroscopic properties. Equilibrium dialysis and gel filtration studies show that tryptophanyl-tRNA synthetase from beef pancreas binds two molecules of L-tryptophan per dimer in an anticooperative way. The binding of tryptophan ellicits a series of spectroscopic changes in the protein as seen by absorbance, fluorescence and circular dichroism. The molar absorption change of the protein-tryptophan system upon formation of the complex is delta epsilon292 = 10 400 +/- 1000 M(-1) cm(-1) per dimer. Taking an initial symmetrical dimeric protein the two dissociation constants for tryptophan at pH 8, 25 degrees C are respectively K1 = 2.0 +/- 0.5 muM and K2 = 10 +/- 4 muM. They are respectively K1 = 1 +/- 0.25 muM and K2 = 20 +/- 8 muM if one considers a sequenced binding of the two tryptophan molecules. The dichroic band at 290 nm of the free protein disappears when tryptophan is bound. All observed changes are characteristic of tryptophan perturbation and none of tyrosine perturbation. They all exceed the effect that can be expected from the change in environment of the bound tryptophan molecules and modifications of the tertiary structure of the protein have to be taken into account to explain the observed spectroscopic data."}
{"id": "PMID:467420", "title": "Changes in the frequency and diversity of messenger RNA populations in the course of myogenic differentiation.", "content": "Complementary DNAs (cDNAs) were synthesized from polyadenylated RNAs of myoblasts and myotubes and used to analyze changes in the sequence complexity and frequency distribution of messenger RNAs during myogenesis in vitro. cDNA . polyadenylated-RNA hybridization kinetics show the presence of messenger RNA sequences specific for myotubes in fully differentiated muscle cultures. These sequences are accumulated just prior to fusion, as was shown by hybridizations of myotube cDNA and total cytoplasmic RNAs from cells at different stages of differentiation. The myotube cDNA can be enriched 10-fold in myotube-specific RNA species by a hybridization with cytoplasmic RNAs from myoblasts and subsequent removal of these hybridized sequences by hydroxyapatite.", "contents": "Changes in the frequency and diversity of messenger RNA populations in the course of myogenic differentiation. Complementary DNAs (cDNAs) were synthesized from polyadenylated RNAs of myoblasts and myotubes and used to analyze changes in the sequence complexity and frequency distribution of messenger RNAs during myogenesis in vitro. cDNA . polyadenylated-RNA hybridization kinetics show the presence of messenger RNA sequences specific for myotubes in fully differentiated muscle cultures. These sequences are accumulated just prior to fusion, as was shown by hybridizations of myotube cDNA and total cytoplasmic RNAs from cells at different stages of differentiation. The myotube cDNA can be enriched 10-fold in myotube-specific RNA species by a hybridization with cytoplasmic RNAs from myoblasts and subsequent removal of these hybridized sequences by hydroxyapatite."}
{"id": "PMID:467421", "title": "Relationship between single-stranded DNA isolated from cultured muscular cells during differentiation and the transcription of messenger RNA.", "content": "Single-stranded DNA (ssDNA), equivalent to about 2% of the total nuclear DNA, was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA of rat myoblast cells and myotubes of the L6 line. Small quantities of 125I-labelled ssDNA were annealed with a large excess of unlabelled DNA, cytoplasmic RNA and mRNA from myoblasts or myotubes. The results indicated that ssDNA belongs to the non-repetitious portion of the cell genome and is formed of two distinct molecular fractions. The major ssDNA fractions (75%) consist of non-self-reassociating DNA sequences and the minor fraction (25%) consists of self-reassociating DNA sequences. About 30--32% and 25--26% of ssDNA from myoblast represent DNA sequences complementary to total cytplasmic RNAs and polyadenylated RNAs respectively. Hybridizations of ssDNA with an excess of RNA from myoblasts and/or myotubes show differences in the abundance and the diversity of mRNA during mascular differentiation. These differences were confirmed by DNA-driven reactions between 125I-labelled polyadenylated RNA and ssDNA in great excess.", "contents": "Relationship between single-stranded DNA isolated from cultured muscular cells during differentiation and the transcription of messenger RNA. Single-stranded DNA (ssDNA), equivalent to about 2% of the total nuclear DNA, was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA of rat myoblast cells and myotubes of the L6 line. Small quantities of 125I-labelled ssDNA were annealed with a large excess of unlabelled DNA, cytoplasmic RNA and mRNA from myoblasts or myotubes. The results indicated that ssDNA belongs to the non-repetitious portion of the cell genome and is formed of two distinct molecular fractions. The major ssDNA fractions (75%) consist of non-self-reassociating DNA sequences and the minor fraction (25%) consists of self-reassociating DNA sequences. About 30--32% and 25--26% of ssDNA from myoblast represent DNA sequences complementary to total cytplasmic RNAs and polyadenylated RNAs respectively. Hybridizations of ssDNA with an excess of RNA from myoblasts and/or myotubes show differences in the abundance and the diversity of mRNA during mascular differentiation. These differences were confirmed by DNA-driven reactions between 125I-labelled polyadenylated RNA and ssDNA in great excess."}
{"id": "PMID:467422", "title": "Photo-induced protein-RNA cross-linking in mammalian 60-S ribosomal subunits.", "content": "Rat liver 60-S ribosomal subunits were submitted to increasing doses of radiation (253.7 nm), at 4 degrees C and 25 degrees C, as previously reported fro 40-S subunits. The existence of protein-RNA cross-linking was demonstrated by two methods. The first consisted in the separation of protein-RNA complex; the second was indirect, and took into account alteration either in the electrophoretic mobility of cross-linked proteins or the separability of 28-S RNA in a 4 M urea/3 M LiCl buffer. The peptide synthetase activity and the sedimentation characteristics of the particles irradiated at 4 degrees C were well preserved, but at 25 degrees C the large subunits were progressively inactivated and unfolded for doses higher than 2 x 10(18) quanta. The dose-dependent variations of protein cross-linkage determined by two-dimensional gel electrophoresis allowed us to distinguish those proteins which reacted at the lowest doses with a first-order reaction from those which cross-linked to RNA after a subtle modification of the subunit structure. At 25 degrees C, all proteins became low-dose reactive. The curve obtained for 28-S RNA cross-linkage was similar to that of the total protein moiety, while those obtained fro the 5-S and 5.8-S RNA (which were parallel) suggest a lower reactivity of these RNAs. As a general rule, proteins from the large subunits were more reactive to RNA than those from the small subunits. This could indicate differences in the organisation of the two subunits.", "contents": "Photo-induced protein-RNA cross-linking in mammalian 60-S ribosomal subunits. Rat liver 60-S ribosomal subunits were submitted to increasing doses of radiation (253.7 nm), at 4 degrees C and 25 degrees C, as previously reported fro 40-S subunits. The existence of protein-RNA cross-linking was demonstrated by two methods. The first consisted in the separation of protein-RNA complex; the second was indirect, and took into account alteration either in the electrophoretic mobility of cross-linked proteins or the separability of 28-S RNA in a 4 M urea/3 M LiCl buffer. The peptide synthetase activity and the sedimentation characteristics of the particles irradiated at 4 degrees C were well preserved, but at 25 degrees C the large subunits were progressively inactivated and unfolded for doses higher than 2 x 10(18) quanta. The dose-dependent variations of protein cross-linkage determined by two-dimensional gel electrophoresis allowed us to distinguish those proteins which reacted at the lowest doses with a first-order reaction from those which cross-linked to RNA after a subtle modification of the subunit structure. At 25 degrees C, all proteins became low-dose reactive. The curve obtained for 28-S RNA cross-linkage was similar to that of the total protein moiety, while those obtained fro the 5-S and 5.8-S RNA (which were parallel) suggest a lower reactivity of these RNAs. As a general rule, proteins from the large subunits were more reactive to RNA than those from the small subunits. This could indicate differences in the organisation of the two subunits."}
{"id": "PMID:467423", "title": "Binding of antibodies against histone H1 to unfolded and folded nucleofilaments.", "content": "Rabbit antibodies to calf thymus histone H1 were purified by affinity chromatography on histone-H1--Sepharose and used as a probe for detecting histone H1 in the nucleofilaments prepared from rat liver nuclei. Binding of the antibodies to the unfolded form of nucleofilaments in 5 mM NaC1 and to the folded form in 80 mM NaC1 Was compared. Sucrose density gradient analyses clearly show that the antibodies can preferentially bind to nucleofilaments in 5 mM NaC1 but not in 80 mM NaC1. The antibodies, however, can bind to mononucleosomes in 80 mM NaC1. These results suggest that antigenic determinants of histone H1 in unfolded nucleofilaments and in mononucleosomes are accessible to the antibodies, while those in folded nucleofilaments are not. This is consistent with the view that histone H1 is used for folding and packing of a nucleosomal chain.", "contents": "Binding of antibodies against histone H1 to unfolded and folded nucleofilaments. Rabbit antibodies to calf thymus histone H1 were purified by affinity chromatography on histone-H1--Sepharose and used as a probe for detecting histone H1 in the nucleofilaments prepared from rat liver nuclei. Binding of the antibodies to the unfolded form of nucleofilaments in 5 mM NaC1 and to the folded form in 80 mM NaC1 Was compared. Sucrose density gradient analyses clearly show that the antibodies can preferentially bind to nucleofilaments in 5 mM NaC1 but not in 80 mM NaC1. The antibodies, however, can bind to mononucleosomes in 80 mM NaC1. These results suggest that antigenic determinants of histone H1 in unfolded nucleofilaments and in mononucleosomes are accessible to the antibodies, while those in folded nucleofilaments are not. This is consistent with the view that histone H1 is used for folding and packing of a nucleosomal chain."}
{"id": "PMID:467424", "title": "DNA polymerase beta from brain neurons is a repair enzyme.", "content": "DNA polymerase beta was isolated from rat cortex neurons and characterised. Its properties were strikingly similar to those of other mammalian beta-polymerases. In adult rats, this was the major DNA polymerase occurring in neuronal nuclei, which contained no alpha-polymerase, 99.2% beta-polymerase and only 0.8% gamma-polymerase. Isolated neuronal nuclei of this developmental stage were shown to perform ultraviolet-induced repair DNA synthesis in vitro. Since beta-polymerase was virtually the exclusive DNA polymerase in these nuclei it was concluded that the beta enzyme was responsible for the observed DNA repair. This was further substantiated by demonstrating a virtually complete suppression of DNA repair in irradiated nuclei by 2',3'-dideoxyribosylthymine 5'-triphosphate (d2TTP), a potent beta-polymerase inhibitor. However, the presence of minute amounts of gamma-polymerase in neuronal nuclei and its susceptibility to d2TTP did not allow one to rule out an ancillary role of DNA polymerase gamma in DNA repair. In view of the similarity of the neuronal DNA polymerase beta with all other mammalian beta-polymerases it may be speculated that the ability to perform repair DNA synthesis is not unique to the neuronal enzyme but is a general function of all beta-polymerases.", "contents": "DNA polymerase beta from brain neurons is a repair enzyme. DNA polymerase beta was isolated from rat cortex neurons and characterised. Its properties were strikingly similar to those of other mammalian beta-polymerases. In adult rats, this was the major DNA polymerase occurring in neuronal nuclei, which contained no alpha-polymerase, 99.2% beta-polymerase and only 0.8% gamma-polymerase. Isolated neuronal nuclei of this developmental stage were shown to perform ultraviolet-induced repair DNA synthesis in vitro. Since beta-polymerase was virtually the exclusive DNA polymerase in these nuclei it was concluded that the beta enzyme was responsible for the observed DNA repair. This was further substantiated by demonstrating a virtually complete suppression of DNA repair in irradiated nuclei by 2',3'-dideoxyribosylthymine 5'-triphosphate (d2TTP), a potent beta-polymerase inhibitor. However, the presence of minute amounts of gamma-polymerase in neuronal nuclei and its susceptibility to d2TTP did not allow one to rule out an ancillary role of DNA polymerase gamma in DNA repair. In view of the similarity of the neuronal DNA polymerase beta with all other mammalian beta-polymerases it may be speculated that the ability to perform repair DNA synthesis is not unique to the neuronal enzyme but is a general function of all beta-polymerases."}
{"id": "PMID:467425", "title": "The effect of octanoate and palmitate on the metabolism of valine in perfused hindquarter of rat.", "content": "The effect of octanoate and palmitate on the oxidation of 14C-labelled valine has been studied in perfused hindquarter of rat. 1. The oxidation rate of valine increases 30 times when the concentration of valine is increased from 0.1 mM to 5 mM. 2. Octanoate at a 5 mM concentration effected a 10-fold increase in the flux through the alpha-ketoisovalerate dehydrogenase step and a 5-fold increase at 0.5 mM concentration. 3. Palmitate (1 mM) effects only a moderate increase in the valine oxidation. 4. With no octanoate there was a great accumulation of alpha-ketoisovalerate in both the muscle and the perfusion medium. 5. With octanoate little alpha-ketoisovalerate accumulated whereas 3-hydroxyisobutyrate was found in high concentration both in the muscle and in the medium. 6. Octanoate stimulated the production of citric-acid-cycle intermediates and lactate. 7. The results are discussed in relation to valine metabolism in the body.", "contents": "The effect of octanoate and palmitate on the metabolism of valine in perfused hindquarter of rat. The effect of octanoate and palmitate on the oxidation of 14C-labelled valine has been studied in perfused hindquarter of rat. 1. The oxidation rate of valine increases 30 times when the concentration of valine is increased from 0.1 mM to 5 mM. 2. Octanoate at a 5 mM concentration effected a 10-fold increase in the flux through the alpha-ketoisovalerate dehydrogenase step and a 5-fold increase at 0.5 mM concentration. 3. Palmitate (1 mM) effects only a moderate increase in the valine oxidation. 4. With no octanoate there was a great accumulation of alpha-ketoisovalerate in both the muscle and the perfusion medium. 5. With octanoate little alpha-ketoisovalerate accumulated whereas 3-hydroxyisobutyrate was found in high concentration both in the muscle and in the medium. 6. Octanoate stimulated the production of citric-acid-cycle intermediates and lactate. 7. The results are discussed in relation to valine metabolism in the body."}
{"id": "PMID:467426", "title": "The excision of N-methyl-N-nitrosourea-induced lesions from the DNA of Chinese hamster cells as measured by the loss of sites sensitive to an enzyme extract that excises 3-methylpurines but not O6-methylguanine.", "content": "An enzyme extract from Micrococcus luteus excises 3-methyladenine and 3-methylguanine but not O6-methylguanine, 7-methylguanine, 1-methyladenine or 7-methyladenine from DNA reacted with N-methyl-N-nitrosourea. The extract was used to detect lesions in the DNA of Chinese hamster cells treated in culture with N-methyl-N-nitrosourea. It was concluded that 3-methyladenine is excised from these cells with a half-life of about 2.3 h.", "contents": "The excision of N-methyl-N-nitrosourea-induced lesions from the DNA of Chinese hamster cells as measured by the loss of sites sensitive to an enzyme extract that excises 3-methylpurines but not O6-methylguanine. An enzyme extract from Micrococcus luteus excises 3-methyladenine and 3-methylguanine but not O6-methylguanine, 7-methylguanine, 1-methyladenine or 7-methyladenine from DNA reacted with N-methyl-N-nitrosourea. The extract was used to detect lesions in the DNA of Chinese hamster cells treated in culture with N-methyl-N-nitrosourea. It was concluded that 3-methyladenine is excised from these cells with a half-life of about 2.3 h."}
{"id": "PMID:467429", "title": "Polymerization of the bacterial elongation factor for protein synthesis, EF-Tu.", "content": "The bacterial elongation factor for protein synthesis, EF-Tu, polymerizes into fibrils at pH 6.0. These fibrils are 0.7 microM in diameter, at least 200 microns in length, and are positively birefringent. Electron microscopic observations of negatively stained images demonstrates that the EF-Tu fibrils consist of bundles of individual filaments, approximately 5nm in diameter, aligned parallel to the long axis of the fibril. Polymerized EF-Tu exchanges nucleotide rapidly and interacts with the other elongation factor, EF-Ts. The antibiotic kirromycin induces the polymerization of EF-Tu into fibrils and even larger structures under nonpolymerizing conditions.", "contents": "Polymerization of the bacterial elongation factor for protein synthesis, EF-Tu. The bacterial elongation factor for protein synthesis, EF-Tu, polymerizes into fibrils at pH 6.0. These fibrils are 0.7 microM in diameter, at least 200 microns in length, and are positively birefringent. Electron microscopic observations of negatively stained images demonstrates that the EF-Tu fibrils consist of bundles of individual filaments, approximately 5nm in diameter, aligned parallel to the long axis of the fibril. Polymerized EF-Tu exchanges nucleotide rapidly and interacts with the other elongation factor, EF-Ts. The antibiotic kirromycin induces the polymerization of EF-Tu into fibrils and even larger structures under nonpolymerizing conditions."}
{"id": "PMID:467431", "title": "Monomer-micelle transition of the ganglioside GM1 and the hydrolysis by Clostridium perfringens neuraminidase.", "content": "The action of Clostridium perfringens neuraminidase on the ganglioside Gm1 tritiated in the ceramide moiety was studied. The rates of hydrolysis of the Gm1 ganglioside were determined from radioactivity in the neutral glycolipid product, which was separated from the substrate on DEAE-Sephadex columns. In order to study the physical state of the substrate in the conditions used in the neuraminidase treatment, the critical micelle concentrations of the Gm1 ganglioside were determined using formation of the triiodide anion in aqueous iodine solution as an indicator. The critical micelle concentrations were also obtained by determining the non-sedimenting radioactivity at different concentrations of the labeled ganglioside per total volume used in ultracentrifugation experiments. In addition, the concentrations of the monomeric ganglioside were concluded from the results of the ultra-centrifugation studies. The increase in the reaction rate of the Gm1 hydrolysis as the function of the substrate concentration was leveled off at 25-28 microM ganglioside. The abrupt change at this concentration is interpreted as reflecting the monomer-micelle transition of the ganglioside in the conditions used (50mM sodium acetate buffer, pH 4.6). The critical micelle concentration was 29 microM on the basis of the triiodide test, and ultracentrifugation revealed the critical micelle concentration 28 microM. The reaction velocity of the hydrolysis was decreased immediately above the critical micelle concentration, and became constant at higher concentrations of the ganglioside. A close correlation to these changes in the reaction rate is suggested to exist in the concentrations of the monomeric Gm1 ganglioside. Saturation of the buffer used in the neuraminidase assays with butanol effected a striking change in the plot of reaction rate versus ganglioside concentration. The reaction rate increased up to 100-110 microM Gm1 ganglioside. The shift of the inflexion point in the rate plot from 25-28 microM to 100-110 microM ganglioside concentration is suggested to be due to a respective change in the critical micelle concentration effected by butanol. N-Acetylneuraminyllactosyl ceramide, lactosyl ceramide and asialo-Gm1 ganglioside had an inhibitory effect on the reaction. In contrast, N-acetylneuraminyllactose, lactose and some other free saccharides were not inhibitory. The results demonstrate that factors other than the saccharide structure must be taken into account when substrate specificity of a glycosidase is studied using competition experiments. It is suggested that the inhibition effected by the glycolipids is due to an increase in the micellar state of the Gm1 ganglioside.", "contents": "Monomer-micelle transition of the ganglioside GM1 and the hydrolysis by Clostridium perfringens neuraminidase. The action of Clostridium perfringens neuraminidase on the ganglioside Gm1 tritiated in the ceramide moiety was studied. The rates of hydrolysis of the Gm1 ganglioside were determined from radioactivity in the neutral glycolipid product, which was separated from the substrate on DEAE-Sephadex columns. In order to study the physical state of the substrate in the conditions used in the neuraminidase treatment, the critical micelle concentrations of the Gm1 ganglioside were determined using formation of the triiodide anion in aqueous iodine solution as an indicator. The critical micelle concentrations were also obtained by determining the non-sedimenting radioactivity at different concentrations of the labeled ganglioside per total volume used in ultracentrifugation experiments. In addition, the concentrations of the monomeric ganglioside were concluded from the results of the ultra-centrifugation studies. The increase in the reaction rate of the Gm1 hydrolysis as the function of the substrate concentration was leveled off at 25-28 microM ganglioside. The abrupt change at this concentration is interpreted as reflecting the monomer-micelle transition of the ganglioside in the conditions used (50mM sodium acetate buffer, pH 4.6). The critical micelle concentration was 29 microM on the basis of the triiodide test, and ultracentrifugation revealed the critical micelle concentration 28 microM. The reaction velocity of the hydrolysis was decreased immediately above the critical micelle concentration, and became constant at higher concentrations of the ganglioside. A close correlation to these changes in the reaction rate is suggested to exist in the concentrations of the monomeric Gm1 ganglioside. Saturation of the buffer used in the neuraminidase assays with butanol effected a striking change in the plot of reaction rate versus ganglioside concentration. The reaction rate increased up to 100-110 microM Gm1 ganglioside. The shift of the inflexion point in the rate plot from 25-28 microM to 100-110 microM ganglioside concentration is suggested to be due to a respective change in the critical micelle concentration effected by butanol. N-Acetylneuraminyllactosyl ceramide, lactosyl ceramide and asialo-Gm1 ganglioside had an inhibitory effect on the reaction. In contrast, N-acetylneuraminyllactose, lactose and some other free saccharides were not inhibitory. The results demonstrate that factors other than the saccharide structure must be taken into account when substrate specificity of a glycosidase is studied using competition experiments. It is suggested that the inhibition effected by the glycolipids is due to an increase in the micellar state of the Gm1 ganglioside."}
{"id": "PMID:467432", "title": "Enzyme memory. Effect of glucose 6-phosphate and temperature on the molecular transition of wheat-germ hexokinase LI.", "content": "A theory is presented that associates burst (orlag) kinetics with the respective concentrations of enzyme initial states X1 and X6 and with the cooperation of a mnemonical enzyme. The theory predicts that for an enzyme with a negative cooperation, decreasing the initial concentration of X1 (or increasing that of X6) tends to increase the induction time. This increase may correspond to a reversal of a burst in a lag. Similarly, if the enzyme has a positive cooperation, decreasing the initial concentration of X6 (or increasing that of X1) increases the induction time. The first case above is expected to apply to wheat germ hexokinase LI, X1 being the form that binds glucose preferentially, and X6 the one that binds glucose 6-phosphate. By changing solely the respective concentrations of the two initial forms, one may expect to modify the pre-steady-state phase but not the steady-state kinetics of the reaction. By jumping the temperature of the enzyme solution from 4 degrees C to 30 degrees C and letting the transconformation ewuilibrium relax for various periods of time before mixing enzyme with the substrates, one can analyse the effect of the relative concentrations of X1 and X6 on the induction time. One can estimate in that way one of the rate constants of the transconformation between the two free enzyme forms. The shorter the incubation time at 30 degrees C then the smaller is the negative induction time (in absolute values). Another possibility of controlling the ratio between the two initial concentrations of the enzyme, is to pre-mix hexokinase with glucose 6-phosphate and to arrange that glucose-6-phosphate concentration, after mixing enzyme and substrates, is held constant whatever the pre-mixing conditions. When wheat germ hexokinase LI is pre-mixed 30 min at 30 degrees C with glucose 6-phosphate before the reaction starts, the burst does not disappear. If, on the other hand, pre-mixing is effected at 4 degrees C the burst is reversed into lag. This result is taken to mean that the equilibrium constant between the two free enzyme forms (the 'circle' and the 'rhombus') is strongly dependent on temperature. A direct study of the effect of glucose 6-phosphate on the conformational equilibrium of wheat germ hexokinase, gives support to this interpretation. If hexokinase is mixed at 4 degrees C with glucose 6-phosphate a slow increase in fluorescence of tryptophanyl residues is observed, which indicates that the 'rhombus' conformation accumulates under these conditions. On the other hand, at 30 degrees C, glucose 6-phosphate does not produce any significant change in the fluorescence of the protein. As expected, these results imply that the equilibrium between the two free enzymes species is freely reversible a 4 degrees C and nearly irreversible at 30 degrees C. The equations derived from the mnemonical model allow fitting or simulation of the experimental results.", "contents": "Enzyme memory. Effect of glucose 6-phosphate and temperature on the molecular transition of wheat-germ hexokinase LI. A theory is presented that associates burst (orlag) kinetics with the respective concentrations of enzyme initial states X1 and X6 and with the cooperation of a mnemonical enzyme. The theory predicts that for an enzyme with a negative cooperation, decreasing the initial concentration of X1 (or increasing that of X6) tends to increase the induction time. This increase may correspond to a reversal of a burst in a lag. Similarly, if the enzyme has a positive cooperation, decreasing the initial concentration of X6 (or increasing that of X1) increases the induction time. The first case above is expected to apply to wheat germ hexokinase LI, X1 being the form that binds glucose preferentially, and X6 the one that binds glucose 6-phosphate. By changing solely the respective concentrations of the two initial forms, one may expect to modify the pre-steady-state phase but not the steady-state kinetics of the reaction. By jumping the temperature of the enzyme solution from 4 degrees C to 30 degrees C and letting the transconformation ewuilibrium relax for various periods of time before mixing enzyme with the substrates, one can analyse the effect of the relative concentrations of X1 and X6 on the induction time. One can estimate in that way one of the rate constants of the transconformation between the two free enzyme forms. The shorter the incubation time at 30 degrees C then the smaller is the negative induction time (in absolute values). Another possibility of controlling the ratio between the two initial concentrations of the enzyme, is to pre-mix hexokinase with glucose 6-phosphate and to arrange that glucose-6-phosphate concentration, after mixing enzyme and substrates, is held constant whatever the pre-mixing conditions. When wheat germ hexokinase LI is pre-mixed 30 min at 30 degrees C with glucose 6-phosphate before the reaction starts, the burst does not disappear. If, on the other hand, pre-mixing is effected at 4 degrees C the burst is reversed into lag. This result is taken to mean that the equilibrium constant between the two free enzyme forms (the 'circle' and the 'rhombus') is strongly dependent on temperature. A direct study of the effect of glucose 6-phosphate on the conformational equilibrium of wheat germ hexokinase, gives support to this interpretation. If hexokinase is mixed at 4 degrees C with glucose 6-phosphate a slow increase in fluorescence of tryptophanyl residues is observed, which indicates that the 'rhombus' conformation accumulates under these conditions. On the other hand, at 30 degrees C, glucose 6-phosphate does not produce any significant change in the fluorescence of the protein. As expected, these results imply that the equilibrium between the two free enzymes species is freely reversible a 4 degrees C and nearly irreversible at 30 degrees C. The equations derived from the mnemonical model allow fitting or simulation of the experimental results."}
{"id": "PMID:467433", "title": "Higher-order structures of chromatin in solution.", "content": "Neutron scatter studies have been made on gently prepared chicken erythrocyte chromatin over a range of ionic strength. At low ionic strength the mass per unit length of the '10 nm nucleofilament corresponds to one nucleosome per 8--12 nm and a DNA packing ratio of between 6 and 9. From the contrast dependence of the cross-section radius of gyration of the nucleofilament the following parameters have been obtained; RgDNA' the cross-section radius of gyration (Rg) when DNA dominates the scatter; RgP, the cross-section Rg when protein dominates the scatter; Rc, the cross-section Rg at infinite contrast and alpha, the constant which describes the dependence of the cross-section Rg on contrast variation. From our understanding of the structure of the core particle, various arrangement of core particles in the nucleofilament have been tested. In models consistent with the above parameters the core particles are arranged edge-to-edge or with the faces of the core particles inclined to within 20 degrees to the axis of the nucleofilament. With increase of ionic strength the transition to the second-order chromatin structure has been followed. This gave the interesting result that above 20 microM NaCL or 0.4 mM MgCL2 the cross-section Rg increases abruptly to about 9 nm with a packing ratio of 0.2 nucleosome/mn and with further increase of ionic strength the Rg increases to 9.5 nm while the packing ratio increases threefold to 0.6 nucleosome/nm. This suggests a family of supercoils of nucleosomes which contract with increasing ionic strength. In its most contracted form the diameter of the hydrated supercoil has been found from the radial distribution function to be 34 nm. Models for the arrangements of core particles in the 34-nm supercoil are discussed.", "contents": "Higher-order structures of chromatin in solution. Neutron scatter studies have been made on gently prepared chicken erythrocyte chromatin over a range of ionic strength. At low ionic strength the mass per unit length of the '10 nm nucleofilament corresponds to one nucleosome per 8--12 nm and a DNA packing ratio of between 6 and 9. From the contrast dependence of the cross-section radius of gyration of the nucleofilament the following parameters have been obtained; RgDNA' the cross-section radius of gyration (Rg) when DNA dominates the scatter; RgP, the cross-section Rg when protein dominates the scatter; Rc, the cross-section Rg at infinite contrast and alpha, the constant which describes the dependence of the cross-section Rg on contrast variation. From our understanding of the structure of the core particle, various arrangement of core particles in the nucleofilament have been tested. In models consistent with the above parameters the core particles are arranged edge-to-edge or with the faces of the core particles inclined to within 20 degrees to the axis of the nucleofilament. With increase of ionic strength the transition to the second-order chromatin structure has been followed. This gave the interesting result that above 20 microM NaCL or 0.4 mM MgCL2 the cross-section Rg increases abruptly to about 9 nm with a packing ratio of 0.2 nucleosome/mn and with further increase of ionic strength the Rg increases to 9.5 nm while the packing ratio increases threefold to 0.6 nucleosome/nm. This suggests a family of supercoils of nucleosomes which contract with increasing ionic strength. In its most contracted form the diameter of the hydrated supercoil has been found from the radial distribution function to be 34 nm. Models for the arrangements of core particles in the 34-nm supercoil are discussed."}
{"id": "PMID:467434", "title": "The mode of inhibitory action by aphidicolin on eukaryotic DNA polymerase alpha.", "content": "The mode of action by aphidicolin on DNA polymerase alpha from the nuclear fraction of sea-urchin blastulae was studied. The inhibition of DNA polymerase alpha by aphidicolin was uncompetive with activated DNA and competitive with the four deoxynucleoside triphosphates using activated DNA as a template-primer. For truncated (residual or limited) DNA synthesis with only three deoxynucleoside triphosphates, aphidicolin inhibited the residual synthesis more strongly in the absence of dCTP than in the absence of each of the other three deoxynucleoside triphosphates. The inhibition was reversed with excess dCTP but not with the other three deoxynucleoside triphosphates. That is, aphidicolin inhibited DNA polymerase alpha by competing with dCTP with a Ki value of 0.5 microgram/ml and by not competing with the other three deoxynucleoside triphosphates. dTMP incorporation with the activated DNA was more sensitive to aphidicolin than dGMP or dTMP incorporation with poly(dC). (dG)12-18 or poly(dA) . (dT)12-18. Similar results were obtained for DNA polymerase alpha (B form) from mouse myeloma MOPC 104E.", "contents": "The mode of inhibitory action by aphidicolin on eukaryotic DNA polymerase alpha. The mode of action by aphidicolin on DNA polymerase alpha from the nuclear fraction of sea-urchin blastulae was studied. The inhibition of DNA polymerase alpha by aphidicolin was uncompetive with activated DNA and competitive with the four deoxynucleoside triphosphates using activated DNA as a template-primer. For truncated (residual or limited) DNA synthesis with only three deoxynucleoside triphosphates, aphidicolin inhibited the residual synthesis more strongly in the absence of dCTP than in the absence of each of the other three deoxynucleoside triphosphates. The inhibition was reversed with excess dCTP but not with the other three deoxynucleoside triphosphates. That is, aphidicolin inhibited DNA polymerase alpha by competing with dCTP with a Ki value of 0.5 microgram/ml and by not competing with the other three deoxynucleoside triphosphates. dTMP incorporation with the activated DNA was more sensitive to aphidicolin than dGMP or dTMP incorporation with poly(dC). (dG)12-18 or poly(dA) . (dT)12-18. Similar results were obtained for DNA polymerase alpha (B form) from mouse myeloma MOPC 104E."}
{"id": "PMID:467435", "title": "A new purification scheme for elongation factor 1 from rabbit reticulocytes and investigation of the homology of the subunits with those of initiation factor 2.", "content": "The aim of this work was to compare the subunits of the elongation factor EF-1 and the initiation factor eIF-2 from rabbit reticulocytes. We devised a simple procedure for the purification of EF-1: stepwise chromatography on heparin-Sepharose, separation of the heavy form by sucrose gradient centrifugation, and a final step of stepwise chromatography on RNA-Sepharose. The heparin-Sepharose column also clearly separated EF-1 and EF-2 within one chromatographic step. The EF-1 was 350-fold puried and the yield was 10%. This preparation showed after electrophoresis on polyacylamide gels in the presence of sodium dodecyl sulfate three bands corresponding to those described by others as the subunits, with Mr of 54000, 49000 and 29200. An additional band of Mr 34000 was present but no others. The 49000-Mr and 34000-Mr bands corresponded exactly in molecular weight to two of three subunits of eIF-2. A more detailed comparison was therefore made of all subunits of EF-1 and eIF-2. This was done by examination of chymotryptic fingerprints on polyacrylamide gel electrophoresis. No evidence for homology between EF-1 and eIF-2 was found. However, the two larger subunits of eIF-2 had a majority of chymotryptic fragments in common, thus indicating some homology between these polypeptides.", "contents": "A new purification scheme for elongation factor 1 from rabbit reticulocytes and investigation of the homology of the subunits with those of initiation factor 2. The aim of this work was to compare the subunits of the elongation factor EF-1 and the initiation factor eIF-2 from rabbit reticulocytes. We devised a simple procedure for the purification of EF-1: stepwise chromatography on heparin-Sepharose, separation of the heavy form by sucrose gradient centrifugation, and a final step of stepwise chromatography on RNA-Sepharose. The heparin-Sepharose column also clearly separated EF-1 and EF-2 within one chromatographic step. The EF-1 was 350-fold puried and the yield was 10%. This preparation showed after electrophoresis on polyacylamide gels in the presence of sodium dodecyl sulfate three bands corresponding to those described by others as the subunits, with Mr of 54000, 49000 and 29200. An additional band of Mr 34000 was present but no others. The 49000-Mr and 34000-Mr bands corresponded exactly in molecular weight to two of three subunits of eIF-2. A more detailed comparison was therefore made of all subunits of EF-1 and eIF-2. This was done by examination of chymotryptic fingerprints on polyacrylamide gel electrophoresis. No evidence for homology between EF-1 and eIF-2 was found. However, the two larger subunits of eIF-2 had a majority of chymotryptic fragments in common, thus indicating some homology between these polypeptides."}
{"id": "PMID:467436", "title": "Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates.", "content": "Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon-producer line, after induction with synthetic double-stranded RNA. The mRNA was translated in a cell-free protein-synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000-Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA.", "contents": "Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates. Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon-producer line, after induction with synthetic double-stranded RNA. The mRNA was translated in a cell-free protein-synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000-Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA."}
{"id": "PMID:467437", "title": "Phosphorylation in vivo of a vaccinia-virus structural protein found associated with the ribosomes from infected cells.", "content": "When vaccinia-virus-infected cells were labeled with radioactive phosphate in the absence of viral gene expression an additional phosphoprotein, containing phosphoserine, was found specifically associated with the ribosomes. The phosphoprotein was removed from the ribosomes following a 0.5 M KCl washing or after EDTA treatment. This additional phosphoprotein was found in infected cells after either a long (3-4 h) or a short (30 min) labeling period; it was detected when the infected cells were incubated in the presence or absence of an inhibitor of RNA or protein synthesis. This phosphoprotein originated from the phosphorylation of vaccinia virion structural protein VP11b (Mr 11,000) at a specific site since only a single major phosphopeptide was obtained after trypsin digestion. This phosphoprotein was also present in purified vaccinia virions labeled with radioactive phosphate. VP11b protein was phosphorylated in vitro by the protein kinase associated with the cores. When the reaction was carried out at an alkaline pH the phosphorylation in vitro occurred at different sites in the protein; at neutral pH the phosphorylation of VP11b was more specific and, as judged by tryptic peptide analysis, occurred mainly at the same site as in the phosphorylation in vivo. A role for the involvement of phosphoprotein VP11b in the establishment of the shut off of host protein synthesis by vaccinia virus is suggested.", "contents": "Phosphorylation in vivo of a vaccinia-virus structural protein found associated with the ribosomes from infected cells. When vaccinia-virus-infected cells were labeled with radioactive phosphate in the absence of viral gene expression an additional phosphoprotein, containing phosphoserine, was found specifically associated with the ribosomes. The phosphoprotein was removed from the ribosomes following a 0.5 M KCl washing or after EDTA treatment. This additional phosphoprotein was found in infected cells after either a long (3-4 h) or a short (30 min) labeling period; it was detected when the infected cells were incubated in the presence or absence of an inhibitor of RNA or protein synthesis. This phosphoprotein originated from the phosphorylation of vaccinia virion structural protein VP11b (Mr 11,000) at a specific site since only a single major phosphopeptide was obtained after trypsin digestion. This phosphoprotein was also present in purified vaccinia virions labeled with radioactive phosphate. VP11b protein was phosphorylated in vitro by the protein kinase associated with the cores. When the reaction was carried out at an alkaline pH the phosphorylation in vitro occurred at different sites in the protein; at neutral pH the phosphorylation of VP11b was more specific and, as judged by tryptic peptide analysis, occurred mainly at the same site as in the phosphorylation in vivo. A role for the involvement of phosphoprotein VP11b in the establishment of the shut off of host protein synthesis by vaccinia virus is suggested."}
{"id": "PMID:467439", "title": "Catalytic activity of aspartate aminotransferase in the crystal. Equilibrium and kinetic analysis.", "content": "Natural substrates and analogs rapidly diffuse through crystals of pig heart mitochondrial aspartate aminotransferase and react at the active sites causing spectral changes that can be measured by single-crystal microspectrophotometry. Dissociation constants for natural substrates and rate constants of transamination for slowly reacting substrates have been determined. A comparison between the data obtained in the crystal and in solution shows that the crystalline enzyme is catalytically competent and that events occurring in the crystal essentially parallel those occurring in solution, even though minor differences have been detected.", "contents": "Catalytic activity of aspartate aminotransferase in the crystal. Equilibrium and kinetic analysis. Natural substrates and analogs rapidly diffuse through crystals of pig heart mitochondrial aspartate aminotransferase and react at the active sites causing spectral changes that can be measured by single-crystal microspectrophotometry. Dissociation constants for natural substrates and rate constants of transamination for slowly reacting substrates have been determined. A comparison between the data obtained in the crystal and in solution shows that the crystalline enzyme is catalytically competent and that events occurring in the crystal essentially parallel those occurring in solution, even though minor differences have been detected."}
{"id": "PMID:467440", "title": "The role of chloroplast-membrane-protein synthesis in the circadian clock. Purification and partial characterization of a polypeptide which is suggested to be involved in the clock.", "content": "A polypeptide (polypeptide P39), which is presumed to involved in the photosynthetic circadian rhythm in the green alga Acetabularia, was purified from the EDTA-insoluble chloroplast membrane fraction by means of preparative dodecylsulfate gel electrophoresis and then partially characterized. The purity of the isolated polypeptide P39 was confirmed by a further electrophoresis on an analytical dodecylsulfate gel and further elucidated by amino-terminal analysis which shows that glycine is the only amino-terminal amino acid of the purified polypeptide material. The molecular weight of the polypeptide P39 was found to be about 39,000 on analytical gel electrophoresis and the value was further supported by those obtained from amino acid composition and peptide mapping. The amino acid composition of polypeptide P39 showed that the proportion of intermediate amino acid groups is high while the proportion of hydrophilic amino acid groups is well balanced by that of hydrophobic amino acid groups, a property characteristic of membrane proteins.", "contents": "The role of chloroplast-membrane-protein synthesis in the circadian clock. Purification and partial characterization of a polypeptide which is suggested to be involved in the clock. A polypeptide (polypeptide P39), which is presumed to involved in the photosynthetic circadian rhythm in the green alga Acetabularia, was purified from the EDTA-insoluble chloroplast membrane fraction by means of preparative dodecylsulfate gel electrophoresis and then partially characterized. The purity of the isolated polypeptide P39 was confirmed by a further electrophoresis on an analytical dodecylsulfate gel and further elucidated by amino-terminal analysis which shows that glycine is the only amino-terminal amino acid of the purified polypeptide material. The molecular weight of the polypeptide P39 was found to be about 39,000 on analytical gel electrophoresis and the value was further supported by those obtained from amino acid composition and peptide mapping. The amino acid composition of polypeptide P39 showed that the proportion of intermediate amino acid groups is high while the proportion of hydrophilic amino acid groups is well balanced by that of hydrophobic amino acid groups, a property characteristic of membrane proteins."}
{"id": "PMID:467444", "title": "Transcription activity of a DNA-protein complex isolated from spinach plastids.", "content": "A DNA . protein complex of about 150 S is isolated from purified spinach chloroplasts by Sepharose 4B gel filtration. A DNA-dependent RNA polymerase activity is found associated with the complex. This DNA protein complex is able to initiate RNA chains in vitro. The RNA synthesis is more dependent on CTP than other nucleoside triphosphates. 50% of the activity is still present with 0.6 M KCl. The temperature optimum occurs between 30 degrees C and 35 degrees C. Rifampicin and rifamycin SV have no inhibitory effect. TNA products have been characterized by gel filtration and by hybridization with chloroplast DNA (ctDNA). At the beginning of transcription DNA products are linked to the transcription complex and are later detached. The molecular weight of the product ranges between 0.07 X 10(6) and 2 X 10(6). A part of the product (3--4%) has a molecular weight higher than 2 X 10(6). No endogenous RNase activity was present during the molecular weight determinations experiments. Hybridization experiments show that at least 75% of the RNA products are hybridizable with ctDNA and that 40% of these products are composed of chloroplast ribosomal RNA, showing that rDNA is preferentially transcribed.", "contents": "Transcription activity of a DNA-protein complex isolated from spinach plastids. A DNA . protein complex of about 150 S is isolated from purified spinach chloroplasts by Sepharose 4B gel filtration. A DNA-dependent RNA polymerase activity is found associated with the complex. This DNA protein complex is able to initiate RNA chains in vitro. The RNA synthesis is more dependent on CTP than other nucleoside triphosphates. 50% of the activity is still present with 0.6 M KCl. The temperature optimum occurs between 30 degrees C and 35 degrees C. Rifampicin and rifamycin SV have no inhibitory effect. TNA products have been characterized by gel filtration and by hybridization with chloroplast DNA (ctDNA). At the beginning of transcription DNA products are linked to the transcription complex and are later detached. The molecular weight of the product ranges between 0.07 X 10(6) and 2 X 10(6). A part of the product (3--4%) has a molecular weight higher than 2 X 10(6). No endogenous RNase activity was present during the molecular weight determinations experiments. Hybridization experiments show that at least 75% of the RNA products are hybridizable with ctDNA and that 40% of these products are composed of chloroplast ribosomal RNA, showing that rDNA is preferentially transcribed."}
{"id": "PMID:467445", "title": "Distribution of polypyrimidine . polypurine segments in DNA from diverse organisms.", "content": "Polypyrimidine . polypurine segments are regions of duplex DNA which contain a highly asymmetric distribution of pyrimidine and purine nucleotides. A polypyrimidine in single-stranded DNA can be detected by its ability to form a complex and poly(A, G) which will bind to hydroxyapatite. We tested DNA from a variety of organisms and found that most contained polypyrimidines. From the shape of the curve relating DNA size to percentage bound to hydroxyapatite, we conclude that polypyrimidine . polypurine segments occur widely in DNA from higher organisms, at intervals of 6000--8000 base pairs throughout the majority of the genome. Lower levels occur in DNA from yeast and Drosophila.", "contents": "Distribution of polypyrimidine . polypurine segments in DNA from diverse organisms. Polypyrimidine . polypurine segments are regions of duplex DNA which contain a highly asymmetric distribution of pyrimidine and purine nucleotides. A polypyrimidine in single-stranded DNA can be detected by its ability to form a complex and poly(A, G) which will bind to hydroxyapatite. We tested DNA from a variety of organisms and found that most contained polypyrimidines. From the shape of the curve relating DNA size to percentage bound to hydroxyapatite, we conclude that polypyrimidine . polypurine segments occur widely in DNA from higher organisms, at intervals of 6000--8000 base pairs throughout the majority of the genome. Lower levels occur in DNA from yeast and Drosophila."}
{"id": "PMID:467446", "title": "Properties of succinylated wheat-germ agglutinin.", "content": "The physicochemical and binding properties of succinylated wheat germ agglutinin are described in comparison with these of unmodified wheat germ agglutinin. Succinylated wheat germ agglutinin is an acidic protein with a pI of 4.0 +/- 0.2 while the native lectin is basic, pI of 8.5. The solubility of succinylated wheat germ agglutinin is about 100 times higher than that of the unmodified lectin at neutral pH. Both lectins are dimeric at pH down to 5, and the dissociation occurs at pH lower than 4.5. The binding of oligosaccharides of N-acetylglucosamine to both lectins is very similar on the basis of fluorescence and phosphorescence studies. The minimal concentration required to agglutinate rabbit red blood cells is about 2 microgram/ml with both lectins and the concentrations of N-acetylglucosamine and di-N-acetylchitobiose which inhibit agglutination are similar with both lectins. The number of succinylated wheat germ agglutinin molecules bound to the surface of mouse thymocytes was ten times lower than that of the unmodified lectin although the apparent binding constant was only slightly different between the two lectins. The dramatic decrease of the apparent number of cell surface receptors upon succinylation of  the lectin is discussed on the basis of the decrease of the isoelectric point and of the acidic properties of the cell surface.", "contents": "Properties of succinylated wheat-germ agglutinin. The physicochemical and binding properties of succinylated wheat germ agglutinin are described in comparison with these of unmodified wheat germ agglutinin. Succinylated wheat germ agglutinin is an acidic protein with a pI of 4.0 +/- 0.2 while the native lectin is basic, pI of 8.5. The solubility of succinylated wheat germ agglutinin is about 100 times higher than that of the unmodified lectin at neutral pH. Both lectins are dimeric at pH down to 5, and the dissociation occurs at pH lower than 4.5. The binding of oligosaccharides of N-acetylglucosamine to both lectins is very similar on the basis of fluorescence and phosphorescence studies. The minimal concentration required to agglutinate rabbit red blood cells is about 2 microgram/ml with both lectins and the concentrations of N-acetylglucosamine and di-N-acetylchitobiose which inhibit agglutination are similar with both lectins. The number of succinylated wheat germ agglutinin molecules bound to the surface of mouse thymocytes was ten times lower than that of the unmodified lectin although the apparent binding constant was only slightly different between the two lectins. The dramatic decrease of the apparent number of cell surface receptors upon succinylation of  the lectin is discussed on the basis of the decrease of the isoelectric point and of the acidic properties of the cell surface."}
{"id": "PMID:467447", "title": "Isolation and characterization of a sulfated glyceroglucolipid from alveolar lavage of rabbit.", "content": "A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE-Sephadex and silicic acid, and thin-layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono-ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H-6Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 3-1,(3)-O-alkyl-2-0-acylglycerol.", "contents": "Isolation and characterization of a sulfated glyceroglucolipid from alveolar lavage of rabbit. A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE-Sephadex and silicic acid, and thin-layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono-ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H-6Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 3-1,(3)-O-alkyl-2-0-acylglycerol."}
{"id": "PMID:467448", "title": "The use of equilibrium density-gradient ultracentrifugation in the isolation and characterisation of glycoproteins with blood group P1 activity from sheep hydatid-cyst fluid.", "content": "Equilibrium density-gradient ultracentrifugation in caesium choride and caesium sulphate has been used in the isolation and fractionation of the glycoproteins specific for blood-group P1 from hydatid cyst fluids. The fractions obtained have distinct and systematic differences specifically related to their buoyant densities, chemical compositions and specific-activities for group P1. High levels of specific-activity were maintained over a large range of chemical compositions. The peptide content varied systematically from 2.5% for the densest fraction to 37% for the least dense fraction. The amino acid composition was essentially constant over all fractions. The proportion of glucosamine decreased and the proportions of galactosamine, mannose and glucose increased with increasing peptide content of the fractions. The data presented suggest the present of oligosaccharide side-chains of various lengths and compositions and/or the presence of oligosaccharide side-chains with very different chemical compositions, of which only some are associated with the specificity for group P1. The properties of the glycoproteins from hydatid cyst fluids have been compared with those of the glycoproteins from human ovarian cysts. Although some similarities have been demonstrated there are significant differences.", "contents": "The use of equilibrium density-gradient ultracentrifugation in the isolation and characterisation of glycoproteins with blood group P1 activity from sheep hydatid-cyst fluid. Equilibrium density-gradient ultracentrifugation in caesium choride and caesium sulphate has been used in the isolation and fractionation of the glycoproteins specific for blood-group P1 from hydatid cyst fluids. The fractions obtained have distinct and systematic differences specifically related to their buoyant densities, chemical compositions and specific-activities for group P1. High levels of specific-activity were maintained over a large range of chemical compositions. The peptide content varied systematically from 2.5% for the densest fraction to 37% for the least dense fraction. The amino acid composition was essentially constant over all fractions. The proportion of glucosamine decreased and the proportions of galactosamine, mannose and glucose increased with increasing peptide content of the fractions. The data presented suggest the present of oligosaccharide side-chains of various lengths and compositions and/or the presence of oligosaccharide side-chains with very different chemical compositions, of which only some are associated with the specificity for group P1. The properties of the glycoproteins from hydatid cyst fluids have been compared with those of the glycoproteins from human ovarian cysts. Although some similarities have been demonstrated there are significant differences."}
{"id": "PMID:467449", "title": "Biochemical research on oogenesis. Transfer RNA is fully charged in the 42-S storage particles of Xenopus laevis oocytes.", "content": "1. Transfer RNA makes up 30-40% of total RNA in previtellogenic oocytes of Xenopus laevis. The bulk of tRNA is associated with 5-S RNA and two proteins in a high-molecular-weight complex sedimenting at 42S. 2. We show here that all kinds of tRNA are present in the 42-S particles and all of them sediment coincidently. Particle tRNA is fully charged in vivo. During purification of the 42-S particles tRNA becomes partially uncharged. When purified particles are incubated in vitro with amino acids and ATP a charging reaction occurs without disruption of the nucleoprotein complex. Many aminoacyl-tRNA synthetases can be shown to co-sediment with the 42-S particles. We conclude that complete aminoacylation of tRNA within the storage particles results from the activity of particle-bound aminoacyl-tRNA synthetases.", "contents": "Biochemical research on oogenesis. Transfer RNA is fully charged in the 42-S storage particles of Xenopus laevis oocytes. 1. Transfer RNA makes up 30-40% of total RNA in previtellogenic oocytes of Xenopus laevis. The bulk of tRNA is associated with 5-S RNA and two proteins in a high-molecular-weight complex sedimenting at 42S. 2. We show here that all kinds of tRNA are present in the 42-S particles and all of them sediment coincidently. Particle tRNA is fully charged in vivo. During purification of the 42-S particles tRNA becomes partially uncharged. When purified particles are incubated in vitro with amino acids and ATP a charging reaction occurs without disruption of the nucleoprotein complex. Many aminoacyl-tRNA synthetases can be shown to co-sediment with the 42-S particles. We conclude that complete aminoacylation of tRNA within the storage particles results from the activity of particle-bound aminoacyl-tRNA synthetases."}
{"id": "PMID:467450", "title": "Hormonal regulation of glucokinase in primary cultures of rat hepatocytes.", "content": "Glucokinase activity in rat hepatocyte cultures declined with a half-time, t1/2, of 32 h during 3 days under serum-free conditions. Addition of insulin and triamcinolone to the culture medium prevented this decay. Glucokinase levels in hepatocytes derived from fasted rats could be elevated from 7.4 to 16.4 mU/mg protein in the presence of insulin and triamcinolone. In 2-day-old cultures glucokinase was induced in the presence of both hormones with a half time, t1/2, of 5.1 h. In cultures treated for 2 days with triamcinolone, insulin induced a 80% increase of glucokinase even in the absence of glucocorticoids. Insulin induction was dependent on protein synthesis but occurred in the absence of RNA synthesis. Glucocorticoid action, however, depended on RNA synthesis suggesting that glucocorticoids control transcription. Insulin evoked half-maximal effects at 3 nM and dexamethasone and triamcinolone at 0.1 and 1 nM respectively. Degradation of glucokinase was initiated in 2-day-old hepatocytes after removal of triamcinolone and insulin. Protein synthesis was essential for the onset of degradation and glucagon did not affect the rate of glucokinase degradation.", "contents": "Hormonal regulation of glucokinase in primary cultures of rat hepatocytes. Glucokinase activity in rat hepatocyte cultures declined with a half-time, t1/2, of 32 h during 3 days under serum-free conditions. Addition of insulin and triamcinolone to the culture medium prevented this decay. Glucokinase levels in hepatocytes derived from fasted rats could be elevated from 7.4 to 16.4 mU/mg protein in the presence of insulin and triamcinolone. In 2-day-old cultures glucokinase was induced in the presence of both hormones with a half time, t1/2, of 5.1 h. In cultures treated for 2 days with triamcinolone, insulin induced a 80% increase of glucokinase even in the absence of glucocorticoids. Insulin induction was dependent on protein synthesis but occurred in the absence of RNA synthesis. Glucocorticoid action, however, depended on RNA synthesis suggesting that glucocorticoids control transcription. Insulin evoked half-maximal effects at 3 nM and dexamethasone and triamcinolone at 0.1 and 1 nM respectively. Degradation of glucokinase was initiated in 2-day-old hepatocytes after removal of triamcinolone and insulin. Protein synthesis was essential for the onset of degradation and glucagon did not affect the rate of glucokinase degradation."}
{"id": "PMID:467451", "title": "Synthesis of cytochrome f by isolated pea chloroplasts.", "content": "Chloroplasts, isolated from the leaves of 7-day-old pea seedlings, were incubated in the light with [35S]methionine or [3H]leucine. After extraction from the washed chloroplast membranes using a mixture of ethyl acetate, ethanol and ammonia, cytochrome f was precipitated with a monospecific antiserum and resolved by gradient polyacrylamide gel electrophoresis in sodium dodecylsulphate. The cytochrome f band was identified by its intrinsic fluorescence in ultraviolet light and was shown to be radioactive by autoradiography or fluorography of dried polyacrylamide gel. One-dimensional peptide mapping of the products of papain hydrolysis confirmed that the radioactivity was an integral part of cytochrome f. The incorporation of [35S]methionine into cytochrome f was inhibited by D(-)threo-chloramphenicol but not by cycloheximide and did not occur in the dark. The synthesis was resistant to ribonuclease. It is concluded that cytochrome f is synthesised in intact isolated pea chloroplasts.", "contents": "Synthesis of cytochrome f by isolated pea chloroplasts. Chloroplasts, isolated from the leaves of 7-day-old pea seedlings, were incubated in the light with [35S]methionine or [3H]leucine. After extraction from the washed chloroplast membranes using a mixture of ethyl acetate, ethanol and ammonia, cytochrome f was precipitated with a monospecific antiserum and resolved by gradient polyacrylamide gel electrophoresis in sodium dodecylsulphate. The cytochrome f band was identified by its intrinsic fluorescence in ultraviolet light and was shown to be radioactive by autoradiography or fluorography of dried polyacrylamide gel. One-dimensional peptide mapping of the products of papain hydrolysis confirmed that the radioactivity was an integral part of cytochrome f. The incorporation of [35S]methionine into cytochrome f was inhibited by D(-)threo-chloramphenicol but not by cycloheximide and did not occur in the dark. The synthesis was resistant to ribonuclease. It is concluded that cytochrome f is synthesised in intact isolated pea chloroplasts."}
{"id": "PMID:467452", "title": "Determination of the kinetic constants of glucosephosphate isomerase by non-linear optimization. Isomerization and anomerization function.", "content": "The rate constants for the anomerization and isomerization reactions catalysed by glucosephosphate isomerase (EC 5.3.1.9) are determined from kinetic measurements by non-linear optimization technique, employing the simplest reaction pathway with only one enzyme-substrate complex.", "contents": "Determination of the kinetic constants of glucosephosphate isomerase by non-linear optimization. Isomerization and anomerization function. The rate constants for the anomerization and isomerization reactions catalysed by glucosephosphate isomerase (EC 5.3.1.9) are determined from kinetic measurements by non-linear optimization technique, employing the simplest reaction pathway with only one enzyme-substrate complex."}
{"id": "PMID:467453", "title": "The karyosphere during late oogenesis in Rana ridibunda.", "content": "The organization of the nucleus in the oocytes of Rana ridibunda was examined during late diplotene at the light and electron microscopic level. At this stage the chromosomes are relatively condensed and assembled in the centre of the nucleus, constituting a karyosphere. The chromosomes here are associated with the central \"protein sphere\" (15--20 microns in diameter), obviously at their telomeres. Numerous nucleoli are accumulated around the chromosomes, forming a karyosphere capsule and contain segregated fibrillar and granular components; structures resembling perinucleolar chromatin and fibrillar bodies (spherules) are associated with the nucleoli. Granules 30 to 40 nm in diameter are seen to surround the fibrillar spherules. \"Nucleolus-like bodies\" consisting of granules 10 to 15 nm in diameter which are embedded in finely fibrillar material are often associated in contact with the chromosomes. The central sphere is an accumulation of annular structures similar to those of the pore complexes of the nuclear envelope. These structures are bound to the chromosome material, the \"nucleolus-like bodies\" and the fibrillar bodies. A participation of \"nucleolus-like bodies\" in the formation of the central sphere is suggested. A possible role of the nuclear protein matrix in the construction of the karyosphere elements is discussed.", "contents": "The karyosphere during late oogenesis in Rana ridibunda. The organization of the nucleus in the oocytes of Rana ridibunda was examined during late diplotene at the light and electron microscopic level. At this stage the chromosomes are relatively condensed and assembled in the centre of the nucleus, constituting a karyosphere. The chromosomes here are associated with the central \"protein sphere\" (15--20 microns in diameter), obviously at their telomeres. Numerous nucleoli are accumulated around the chromosomes, forming a karyosphere capsule and contain segregated fibrillar and granular components; structures resembling perinucleolar chromatin and fibrillar bodies (spherules) are associated with the nucleoli. Granules 30 to 40 nm in diameter are seen to surround the fibrillar spherules. \"Nucleolus-like bodies\" consisting of granules 10 to 15 nm in diameter which are embedded in finely fibrillar material are often associated in contact with the chromosomes. The central sphere is an accumulation of annular structures similar to those of the pore complexes of the nuclear envelope. These structures are bound to the chromosome material, the \"nucleolus-like bodies\" and the fibrillar bodies. A participation of \"nucleolus-like bodies\" in the formation of the central sphere is suggested. A possible role of the nuclear protein matrix in the construction of the karyosphere elements is discussed."}
{"id": "PMID:467454", "title": "On the association of centrioles with the interphase nucleus.", "content": "Preparations of nuclei from rat liver and bovine spleen purified by centrifugation through dense sucrose solutions are shown to contain centrioles. These centrioles retain their in situ ultrastructure and are surrounded by a network of filaments adjacent to the nucleus and probably attached to it. The number of centrioles in isolated nuclei depends on the conditions of cell homogenization. Under certain conditions of homogenization, the fraction of purified nuclei contains almost all centrioles of the original tissue. The number of centrioles in isolated nuclei sharply decreases if the nuclei are rehomogenized under conditions that do not cause damage to nuclei. The number of nucleus-associated centrioles does not decrease after solubilization of nuclear membranes by Triton X-100. Nuclei retain the associated centrioles after treatmentwith RNase-free DNase I. It is concluded that in interphase the centrioles are associated with the nucleus and that this association which is probably mediated by filaments involves nuclear structures other than nuclear membranes or whole chromatin.", "contents": "On the association of centrioles with the interphase nucleus. Preparations of nuclei from rat liver and bovine spleen purified by centrifugation through dense sucrose solutions are shown to contain centrioles. These centrioles retain their in situ ultrastructure and are surrounded by a network of filaments adjacent to the nucleus and probably attached to it. The number of centrioles in isolated nuclei depends on the conditions of cell homogenization. Under certain conditions of homogenization, the fraction of purified nuclei contains almost all centrioles of the original tissue. The number of centrioles in isolated nuclei sharply decreases if the nuclei are rehomogenized under conditions that do not cause damage to nuclei. The number of nucleus-associated centrioles does not decrease after solubilization of nuclear membranes by Triton X-100. Nuclei retain the associated centrioles after treatmentwith RNase-free DNase I. It is concluded that in interphase the centrioles are associated with the nucleus and that this association which is probably mediated by filaments involves nuclear structures other than nuclear membranes or whole chromatin."}
{"id": "PMID:467455", "title": "Cell cycle dependent changes of chromosomes in mouse fibroblasts.", "content": "Mouse fibroblast interphase nuclei stained with quinacrine dihydrochloride show distinctive differences in their fluorescent characteristics analogous to those which we have already observed in human and Syrian hamster cells. These patterns reflect the position of any given nucleus within the cell cycle. The brightly fluorescent chromocenters in the mouse nuclei were found to be in absolute aggreement with those stained by the C-banding technique, indicating that they represent centromeric heterochromatin. Furthermore, their number and size per nucleus were shown to vary in relation to the progress of the cell cycle.", "contents": "Cell cycle dependent changes of chromosomes in mouse fibroblasts. Mouse fibroblast interphase nuclei stained with quinacrine dihydrochloride show distinctive differences in their fluorescent characteristics analogous to those which we have already observed in human and Syrian hamster cells. These patterns reflect the position of any given nucleus within the cell cycle. The brightly fluorescent chromocenters in the mouse nuclei were found to be in absolute aggreement with those stained by the C-banding technique, indicating that they represent centromeric heterochromatin. Furthermore, their number and size per nucleus were shown to vary in relation to the progress of the cell cycle."}
{"id": "PMID:467456", "title": "Intermitochondrial bridge junctions in fat body cells of the pre-hatch larvae of the forest tent caterpillar Malacosoma disstria HBN.", "content": "An electron microscopic feature of fat body cells of diapausing Malacosoma disstria pre-hatch caterpillars, experimentally held at certain controlled time-and-temperature conditions, is an elaborate bridge junction, between mitochondria, strikingly similar in appearance to the septate junction which has frequently been described as occurring between cells of many invertebrate species. It is suggested that the intermitochondrial unctions may well represent elaborate forms of interaction between organelles in order to enhance metabolic efficiency in times of stress.", "contents": "Intermitochondrial bridge junctions in fat body cells of the pre-hatch larvae of the forest tent caterpillar Malacosoma disstria HBN. An electron microscopic feature of fat body cells of diapausing Malacosoma disstria pre-hatch caterpillars, experimentally held at certain controlled time-and-temperature conditions, is an elaborate bridge junction, between mitochondria, strikingly similar in appearance to the septate junction which has frequently been described as occurring between cells of many invertebrate species. It is suggested that the intermitochondrial unctions may well represent elaborate forms of interaction between organelles in order to enhance metabolic efficiency in times of stress."}
{"id": "PMID:467457", "title": "Effects of 5-bromo-deoxyuridine on chloroplast structure in gametophytes of Anemia phyllitidis L. Sw.", "content": "Application of 5-bromo-2-deoxyuridine causes servere changes in the ultrastructure of chloroplasts. These modifications include an increase of thylakoid layers (\"giant\"-grana), coiling of thylakoids, and lastly vacuolization of the entire membrane system of the chloroplast. Such observations can be correlated with types of membrane aberrations reported for natural mutants.", "contents": "Effects of 5-bromo-deoxyuridine on chloroplast structure in gametophytes of Anemia phyllitidis L. Sw. Application of 5-bromo-2-deoxyuridine causes servere changes in the ultrastructure of chloroplasts. These modifications include an increase of thylakoid layers (\"giant\"-grana), coiling of thylakoids, and lastly vacuolization of the entire membrane system of the chloroplast. Such observations can be correlated with types of membrane aberrations reported for natural mutants."}
{"id": "PMID:467458", "title": "Apparent heterogeneity of hepatic lysosomes due to membrane-bound acid phosphatase.", "content": "Hepatic lysosomes have been fractionated by rate sedimentation and by isopycnic banding. In all experiments, the distribution of acid phosphatase differed from that of the other lysosomal enzymes. Evidence is presented that this difference is due not to the separation of lysosomes from different cell types, but simply reflects the membrane location of a part of the acid phosphatase.", "contents": "Apparent heterogeneity of hepatic lysosomes due to membrane-bound acid phosphatase. Hepatic lysosomes have been fractionated by rate sedimentation and by isopycnic banding. In all experiments, the distribution of acid phosphatase differed from that of the other lysosomal enzymes. Evidence is presented that this difference is due not to the separation of lysosomes from different cell types, but simply reflects the membrane location of a part of the acid phosphatase."}
{"id": "PMID:467459", "title": "[Identification of neurosecretory reservoirs in the mayfly Ephemera danica M\u00fcll. (ephemeroptera: ephemeridae) (author's transl)].", "content": "Neurosecretory reservoirs were found in the head of Ephemera danica. In the region where the nervus corporis cardiaci 1 is leaving the brain, the diameter of this nerve increases considerably. Different types of axons were observed, filled with great amounts of electron opaque secretory granules with mean diameters of 170 nm and 110 nm, respectively. Another neurosecretory reservoir is situated in the rostral part of the corpus cardiacum containing a secretory material of a different kind. Most of the granules have diameters of about 90 nm, and are electron opaque. A relatively small number of axons contains nearly electron lucent vesicles with the same mean diameter.", "contents": "[Identification of neurosecretory reservoirs in the mayfly Ephemera danica M\u00fcll. (ephemeroptera: ephemeridae) (author's transl)]. Neurosecretory reservoirs were found in the head of Ephemera danica. In the region where the nervus corporis cardiaci 1 is leaving the brain, the diameter of this nerve increases considerably. Different types of axons were observed, filled with great amounts of electron opaque secretory granules with mean diameters of 170 nm and 110 nm, respectively. Another neurosecretory reservoir is situated in the rostral part of the corpus cardiacum containing a secretory material of a different kind. Most of the granules have diameters of about 90 nm, and are electron opaque. A relatively small number of axons contains nearly electron lucent vesicles with the same mean diameter."}
{"id": "PMID:467460", "title": "Fine structure of a spider joint receptor and associated synapses.", "content": "The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one branching dendrite into the hypodermis underlying the joint membrane. All dendritic branches together form a sheet-like meshwork 50 microns wide and 1 microns thick, which is traversed obliquely by hypodermis cells. When the joint is stretched shearing forces are apparently transmitted to the receptive dendritic branches via microtubular bundles inside the hypodermis cells. The soma and dendrites of the sensory cells receive numerous synaptic input from presumably efferent fibres. The fine structure of these synapses is described and compared with other peripheral and central spider synapses. All R10 synapses contain small synaptic vesicles (32 nm diameter), whereas motor endplates possess large vesicles (38 nm). Central synapses have two significantly different vesicle populations which are either of the small or large variety. Since synapses with small vesicles are supposedly inhibitory, receptor cells in spiders might be under efferent control. Such a system is unknown in insects or crustaceans, but may be typical for arachnids.", "contents": "Fine structure of a spider joint receptor and associated synapses. The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one branching dendrite into the hypodermis underlying the joint membrane. All dendritic branches together form a sheet-like meshwork 50 microns wide and 1 microns thick, which is traversed obliquely by hypodermis cells. When the joint is stretched shearing forces are apparently transmitted to the receptive dendritic branches via microtubular bundles inside the hypodermis cells. The soma and dendrites of the sensory cells receive numerous synaptic input from presumably efferent fibres. The fine structure of these synapses is described and compared with other peripheral and central spider synapses. All R10 synapses contain small synaptic vesicles (32 nm diameter), whereas motor endplates possess large vesicles (38 nm). Central synapses have two significantly different vesicle populations which are either of the small or large variety. Since synapses with small vesicles are supposedly inhibitory, receptor cells in spiders might be under efferent control. Such a system is unknown in insects or crustaceans, but may be typical for arachnids."}
{"id": "PMID:467461", "title": "Normal versus alpha-amanitin induced cellular dynamics of the midgut epithelium in female Aedes aegypti L. (Insecta, Diptera) in response to blood feeding.", "content": "In midgut epithelial cells (stomach) of untreated female A. aegypti an increase in the surface area of the rough endoplasmic reticulum (rer) and in the ratio of membrane-bound to free ribosomes is morphometrically measured during digestion of the first blood meal. This can be correlated with the synthesis and release of digestive proteases. The dynamics of the ribosomes in A. aegypti are similar to those in A. stephensi. 3 ng alpha-amanitin per mosquito prevent normal blood digestion, the proliferation of the rer and the increase in the ratio of bound to free ribosomes. On the other hand, some synthesis of new ribosomes takes place.", "contents": "Normal versus alpha-amanitin induced cellular dynamics of the midgut epithelium in female Aedes aegypti L. (Insecta, Diptera) in response to blood feeding. In midgut epithelial cells (stomach) of untreated female A. aegypti an increase in the surface area of the rough endoplasmic reticulum (rer) and in the ratio of membrane-bound to free ribosomes is morphometrically measured during digestion of the first blood meal. This can be correlated with the synthesis and release of digestive proteases. The dynamics of the ribosomes in A. aegypti are similar to those in A. stephensi. 3 ng alpha-amanitin per mosquito prevent normal blood digestion, the proliferation of the rer and the increase in the ratio of bound to free ribosomes. On the other hand, some synthesis of new ribosomes takes place."}
{"id": "PMID:467462", "title": "Differences in surface morphology of microtubules reconstituted from pure brain tubulin using two different microtubule-associated proteins: the high molecular weight MAP 2 proteins and tau proteins.", "content": "Microtubules were reconstituted from homogeneous brain tubulin and homogeneous preparations of two different microtubule associated proteins, the high molecular weight MAP 2 proteins or the tau proteins. The resulting microtubules were characterized by three electron microscopical procedures: Thin sectional analysis of embeded material, negative staining analysis using a STEM microscope and high resolution metal-shadowing analysis. By all three procedures MAP 2 microtubules have a much rougher surface morphology than tau microtubules, in agreement with the much higher molecular weight of the MAP 2 proteins. Tau microtubules, however, do not show the very smooth surface of microtubules assembled from pure tubulin in the absence of any microtubule associated proteins. In the case of MAP 2 microtubules thin sectional analysis as well as metal shadowing reveals that the globular protrusions seen in negative staining analysis appear as linear side arms which may extend by as much as 30 nm on both sides from the microtubular wall proper, giving rise to an overall structure with a diameter close to 100 nm. The possible implication of such structures for in vivo situations is briefly discussed as is the possibility that the \"halo-effect\" around microtubules seen in vivo may be due to a structural organization similar to that of MAP 2 tubules in vitro.", "contents": "Differences in surface morphology of microtubules reconstituted from pure brain tubulin using two different microtubule-associated proteins: the high molecular weight MAP 2 proteins and tau proteins. Microtubules were reconstituted from homogeneous brain tubulin and homogeneous preparations of two different microtubule associated proteins, the high molecular weight MAP 2 proteins or the tau proteins. The resulting microtubules were characterized by three electron microscopical procedures: Thin sectional analysis of embeded material, negative staining analysis using a STEM microscope and high resolution metal-shadowing analysis. By all three procedures MAP 2 microtubules have a much rougher surface morphology than tau microtubules, in agreement with the much higher molecular weight of the MAP 2 proteins. Tau microtubules, however, do not show the very smooth surface of microtubules assembled from pure tubulin in the absence of any microtubule associated proteins. In the case of MAP 2 microtubules thin sectional analysis as well as metal shadowing reveals that the globular protrusions seen in negative staining analysis appear as linear side arms which may extend by as much as 30 nm on both sides from the microtubular wall proper, giving rise to an overall structure with a diameter close to 100 nm. The possible implication of such structures for in vivo situations is briefly discussed as is the possibility that the \"halo-effect\" around microtubules seen in vivo may be due to a structural organization similar to that of MAP 2 tubules in vitro."}
{"id": "PMID:467463", "title": "The estimation of velocity distribution profile of Paramecium cytoplasmic streaming.", "content": "Cytoplasmic streaming of Paramecium bursaria and Paramecium tetraurelia was investigated by cinematographic techniques. Analysis of the records reveals the paraboidal character of the velocity distribution profiles in all arbitrarily chosen zones along the whole route of moving cytoplasm in the cell. According to the date obtained from cytoplasmic streaming analysis and food vacuole path, the geometry of the \"channel\" was described in terms of ellipsoid axes. Total volume changes of cytoplasm flowing through a given cross section in a given time unit were computed and no significant differences were found. The participation of a pressure gradient in motive force generation in cytoplasmic streaming is discussed.", "contents": "The estimation of velocity distribution profile of Paramecium cytoplasmic streaming. Cytoplasmic streaming of Paramecium bursaria and Paramecium tetraurelia was investigated by cinematographic techniques. Analysis of the records reveals the paraboidal character of the velocity distribution profiles in all arbitrarily chosen zones along the whole route of moving cytoplasm in the cell. According to the date obtained from cytoplasmic streaming analysis and food vacuole path, the geometry of the \"channel\" was described in terms of ellipsoid axes. Total volume changes of cytoplasm flowing through a given cross section in a given time unit were computed and no significant differences were found. The participation of a pressure gradient in motive force generation in cytoplasmic streaming is discussed."}
{"id": "PMID:467464", "title": "Alterations of crossbridge angle induced by beta, gamma-imido-adenosine-triphosphate. Electron microscope and optical diffraction studies on myofibrillar fragments of abdominal muscles of the crayfish Orconectes limosus.", "content": "The molecular basis of muscle contraction is thought to consist of cyclic movements of parts of the myosin molecules (crossbridges). Unitl now different states of the proposed crossbridge cycle could be stablilized and demonstrated by electron microscopy only in the case of highly specialized insect flight muscles. In this paper evidence is presented that it is also possible to induce crossbridge positions corresponding to the rigor [16] and the pseudorelaxed state [3] in non-insect muscles. Homogenization of myofibrils of the abdominal flexors of the crayfish Orconectes limosus in rigor or AMP.PNP-containing solutions brings about two different crossbridge patterns: The formation of crossbridges attached to the actin filaments in a mainly acute (rigor) or in a mainly perpendicular angle (pseudo-relaxed). Optical diffraction patterns taken from electron micrographs of sarcomere fragments are likewise compatible with those taken from sarcomeres of insect flight muscles fixed in comparable conditions [2,3].", "contents": "Alterations of crossbridge angle induced by beta, gamma-imido-adenosine-triphosphate. Electron microscope and optical diffraction studies on myofibrillar fragments of abdominal muscles of the crayfish Orconectes limosus. The molecular basis of muscle contraction is thought to consist of cyclic movements of parts of the myosin molecules (crossbridges). Unitl now different states of the proposed crossbridge cycle could be stablilized and demonstrated by electron microscopy only in the case of highly specialized insect flight muscles. In this paper evidence is presented that it is also possible to induce crossbridge positions corresponding to the rigor [16] and the pseudorelaxed state [3] in non-insect muscles. Homogenization of myofibrils of the abdominal flexors of the crayfish Orconectes limosus in rigor or AMP.PNP-containing solutions brings about two different crossbridge patterns: The formation of crossbridges attached to the actin filaments in a mainly acute (rigor) or in a mainly perpendicular angle (pseudo-relaxed). Optical diffraction patterns taken from electron micrographs of sarcomere fragments are likewise compatible with those taken from sarcomeres of insect flight muscles fixed in comparable conditions [2,3]."}
{"id": "PMID:467465", "title": "Phenytoin binding to human albumin.", "content": "Binding of phenytoin to human plasma proteins and to human serum albumin is studied using equilibrium dialysis method at pH 7.4 and 37 degrees C. Phenytoin is mainly bound to albumin, the percentage of bound drug being constant over a wide range of total drug concentrations. Calculation of the drug binding parameters show a low affinity, k = 745 M-1, and a high number of binding sites, n = 8. Palmitic acid and some acidic drugs, warfarin and phenylbutazone added to human serum albumin, decreased phenytoin binding in a non competitive way. Basic and non-ionizable drugs, on the other hand, did not modify phenytoin binding.", "contents": "Phenytoin binding to human albumin. Binding of phenytoin to human plasma proteins and to human serum albumin is studied using equilibrium dialysis method at pH 7.4 and 37 degrees C. Phenytoin is mainly bound to albumin, the percentage of bound drug being constant over a wide range of total drug concentrations. Calculation of the drug binding parameters show a low affinity, k = 745 M-1, and a high number of binding sites, n = 8. Palmitic acid and some acidic drugs, warfarin and phenylbutazone added to human serum albumin, decreased phenytoin binding in a non competitive way. Basic and non-ionizable drugs, on the other hand, did not modify phenytoin binding."}
{"id": "PMID:467466", "title": "In vitro studies on the metabolism of dibenzo[c,f]-[1,2]diazepine.", "content": "Dibenzo [c,f]-[1,2] diazepine is a new compound with a tricyclic structure and a double bond between two nitrogen atoms in positions 1 and 2 of the molecule. Its in vitro metabolism has been studied, incubating the compound with rat liver microsomes. Two metabolites have been identified: dibenzo [c,f] - [1,2]diazepine-1-N-oxide and a monohydroxyderivative in the aromatic ring.", "contents": "In vitro studies on the metabolism of dibenzo[c,f]-[1,2]diazepine. Dibenzo [c,f]-[1,2] diazepine is a new compound with a tricyclic structure and a double bond between two nitrogen atoms in positions 1 and 2 of the molecule. Its in vitro metabolism has been studied, incubating the compound with rat liver microsomes. Two metabolites have been identified: dibenzo [c,f] - [1,2]diazepine-1-N-oxide and a monohydroxyderivative in the aromatic ring."}
{"id": "PMID:467467", "title": "Pharmacokinetics of intravenous amikacin after rapid and slow infusion with special reference to hemodialysis.", "content": "The pharmacokinetics of amikacin, a recently introduced aminoglycoside structurally related to kanamycin, were determined in healthy volunteers after rapid and slow constant-rate intravenous administration of a 7.5 mg/kg dose. The elimination profile of amikacin can be described by two compartment open model kinetics. Peripheral distribution of the drug is extremely rapid, and beta-phase concentrations decay with a half-life averaging about 2 hours, while inter-compartmental equilibrium is achieved in a little over 30 minutes. The volume of distribution averages about 25% of body weight. During hemodialysis, amikacin extraction from the blood reaches 97% +/- 17% (mean +/- 95% confidence interval) that of creatinine and 89% +/- 20% that of blood urea nitrogen. A method of administration adapted to the kinetic properties of the antibiotic is proposed.", "contents": "Pharmacokinetics of intravenous amikacin after rapid and slow infusion with special reference to hemodialysis. The pharmacokinetics of amikacin, a recently introduced aminoglycoside structurally related to kanamycin, were determined in healthy volunteers after rapid and slow constant-rate intravenous administration of a 7.5 mg/kg dose. The elimination profile of amikacin can be described by two compartment open model kinetics. Peripheral distribution of the drug is extremely rapid, and beta-phase concentrations decay with a half-life averaging about 2 hours, while inter-compartmental equilibrium is achieved in a little over 30 minutes. The volume of distribution averages about 25% of body weight. During hemodialysis, amikacin extraction from the blood reaches 97% +/- 17% (mean +/- 95% confidence interval) that of creatinine and 89% +/- 20% that of blood urea nitrogen. A method of administration adapted to the kinetic properties of the antibiotic is proposed."}
{"id": "PMID:467468", "title": "Comparative distribution of two antidepressant drugs (imipramine and indalpine) in the rat as determined by analog computer simulation.", "content": "A ten-compartment analog computer model is presented to determine the precise distribution and excretion of two antidepressant drugs, LM 5008 AND Imipramine. Eight patterns were simulated using experimental data and drug distribution in the two undetermined compartments were obtained by the analog model. Close agreement with existing experimental data lends confidence in the model as a valuable tool for predictions in a variety of therapeutic situations.", "contents": "Comparative distribution of two antidepressant drugs (imipramine and indalpine) in the rat as determined by analog computer simulation. A ten-compartment analog computer model is presented to determine the precise distribution and excretion of two antidepressant drugs, LM 5008 AND Imipramine. Eight patterns were simulated using experimental data and drug distribution in the two undetermined compartments were obtained by the analog model. Close agreement with existing experimental data lends confidence in the model as a valuable tool for predictions in a variety of therapeutic situations."}
{"id": "PMID:467469", "title": "Lesions of the dorsal renal artery in surgery for renal pelvic calculus. A potential cause of renovascular hypertension.", "content": "2 cases of lesions of the dorsal renal artery in surgery for renal pelvic calculus are described. In 1 of the cases hypertension developed. Anatomic factors and previous operations predispose to this complication. An arterial complication should be suspected when hypertension develops postoperatively. The blood pressure of patients with known renal vascular complications should be checked repeatedly for several months postoperatively. The diagnosis is radiological, renal angiography being the definite procedure although a reduction of kidney size observed at urography may indicate the vascular lesion.", "contents": "Lesions of the dorsal renal artery in surgery for renal pelvic calculus. A potential cause of renovascular hypertension. 2 cases of lesions of the dorsal renal artery in surgery for renal pelvic calculus are described. In 1 of the cases hypertension developed. Anatomic factors and previous operations predispose to this complication. An arterial complication should be suspected when hypertension develops postoperatively. The blood pressure of patients with known renal vascular complications should be checked repeatedly for several months postoperatively. The diagnosis is radiological, renal angiography being the definite procedure although a reduction of kidney size observed at urography may indicate the vascular lesion."}
{"id": "PMID:467470", "title": "Ureteroureterostomy in complete ureteral duplication.", "content": "Homolateral pelvic ureteroureterostomies were performed in 25 patients presenting with complete duplication of the pyeloureteric tract. The procedure was performed in 16 cases of ureteroceles, in 7 cases of reflux in duplicated ureters, in 1 case of ectopic ureter and in 1 postoperative stenosis of one segment of a double ureter. According to the authors' experience this is the surgical treatment of choice in conservative surgical treatment.", "contents": "Ureteroureterostomy in complete ureteral duplication. Homolateral pelvic ureteroureterostomies were performed in 25 patients presenting with complete duplication of the pyeloureteric tract. The procedure was performed in 16 cases of ureteroceles, in 7 cases of reflux in duplicated ureters, in 1 case of ectopic ureter and in 1 postoperative stenosis of one segment of a double ureter. According to the authors' experience this is the surgical treatment of choice in conservative surgical treatment."}
{"id": "PMID:467471", "title": "Regeneration of the ureter and the renal pelvis in dogs from transversely transected and anastomosed strips of the ureteral wall. Influence of long administration of azathioprine and prednisone on the newly formed wall. I. Experimental.", "content": "Regeneration of the ureter and renal pelvis from a transected and anastomosed strip of the ureteral wall was examined in 20 dogs. The latter were divided into two groups: group A, dogs operated without receiving any drug, and group B, dogs which received 1.5 mg prednisone and 1.5 azathioprine/kg body weight/day before the operation and throughout the postoperative period. Provided the ureteral splint was maintained for at least 28 days, good results were obtained in both groups. Long-term administration of the drugs thus had no adverse effects on the regeneration. Microscopic examination of the newly formed wall, showed more numerous and better arranged muscular fibers and less connective tissues in group B.", "contents": "Regeneration of the ureter and the renal pelvis in dogs from transversely transected and anastomosed strips of the ureteral wall. Influence of long administration of azathioprine and prednisone on the newly formed wall. I. Experimental. Regeneration of the ureter and renal pelvis from a transected and anastomosed strip of the ureteral wall was examined in 20 dogs. The latter were divided into two groups: group A, dogs operated without receiving any drug, and group B, dogs which received 1.5 mg prednisone and 1.5 azathioprine/kg body weight/day before the operation and throughout the postoperative period. Provided the ureteral splint was maintained for at least 28 days, good results were obtained in both groups. Long-term administration of the drugs thus had no adverse effects on the regeneration. Microscopic examination of the newly formed wall, showed more numerous and better arranged muscular fibers and less connective tissues in group B."}
{"id": "PMID:467472", "title": "Modulatory effects of oestrogen on immunologic responsiveness. I. Inhibition of DNA synthesis in peripheral blood lymphocytes from patients with Peyronie's disease, prostatic cancer and transsexuals and a commentary.", "content": "A reduction in the phytohaemagglutinin-induced transformation of peripheral blood lymphocytes from patients with Peyronie's disease and prostate cancer and transsexuals cultured in autologous and homologous serum following the receipt of oestrogen therapy has been  observed. Reduction of lymphocyte transformation in patients with prostatic cancer and without malignancy receiving oestrogen, i.e., Peyronie's disease and transsexuals, suggests that this reduction is related to the mode of therapy rather than to malignancy or a particular pathologic state. No direct evidence exists that the observed in vitro aberrations of lymphocytic responsiveness are reflective of host compromise, but these observations are of potential relevance in terms of their implications in the therapeutic management of patients with prostatic cancer and other hormonally-dependent tumours, e.g., of the breast, as well as responsive diseases, through their effect on cellular immunocompetence and suitability of hormonally treated patients as prospective candidates for adjuvant immunotherapy. The possible relevance of the present observations to the suggested association between uterine cancer and prolonged administration of diethylstilboesterol, as well as that between development of vaginal tumours in offspring and maternal ingestion during pregnancy, also remains of potential concern, pending further investigation.", "contents": "Modulatory effects of oestrogen on immunologic responsiveness. I. Inhibition of DNA synthesis in peripheral blood lymphocytes from patients with Peyronie's disease, prostatic cancer and transsexuals and a commentary. A reduction in the phytohaemagglutinin-induced transformation of peripheral blood lymphocytes from patients with Peyronie's disease and prostate cancer and transsexuals cultured in autologous and homologous serum following the receipt of oestrogen therapy has been  observed. Reduction of lymphocyte transformation in patients with prostatic cancer and without malignancy receiving oestrogen, i.e., Peyronie's disease and transsexuals, suggests that this reduction is related to the mode of therapy rather than to malignancy or a particular pathologic state. No direct evidence exists that the observed in vitro aberrations of lymphocytic responsiveness are reflective of host compromise, but these observations are of potential relevance in terms of their implications in the therapeutic management of patients with prostatic cancer and other hormonally-dependent tumours, e.g., of the breast, as well as responsive diseases, through their effect on cellular immunocompetence and suitability of hormonally treated patients as prospective candidates for adjuvant immunotherapy. The possible relevance of the present observations to the suggested association between uterine cancer and prolonged administration of diethylstilboesterol, as well as that between development of vaginal tumours in offspring and maternal ingestion during pregnancy, also remains of potential concern, pending further investigation."}
{"id": "PMID:467473", "title": "Pelvic lipomatosis: a nonsurgical diagnosis.", "content": "Pelvic lipomatosis is rare in Europe. Preoperative diagnosis is seldom reached and subsequent explorative procedures are necessary. Typical roentgenographic findings are presented. The possibility of an early presurgical diagnosis is emphasized.", "contents": "Pelvic lipomatosis: a nonsurgical diagnosis. Pelvic lipomatosis is rare in Europe. Preoperative diagnosis is seldom reached and subsequent explorative procedures are necessary. Typical roentgenographic findings are presented. The possibility of an early presurgical diagnosis is emphasized."}
{"id": "PMID:467474", "title": "Hypertensive renal rhabdomyosarcoma.", "content": "A case of renal rhabdomyosarcoma is presented. Besides the rarity of this tumor, the case is unique because an increased level of renin and hypertension was found, which was not due to compression of the main vessels.", "contents": "Hypertensive renal rhabdomyosarcoma. A case of renal rhabdomyosarcoma is presented. Besides the rarity of this tumor, the case is unique because an increased level of renin and hypertension was found, which was not due to compression of the main vessels."}
{"id": "PMID:467481", "title": "Relationships between hemodynamic profiles and topography of acute myocardial infarction.", "content": "Initial hemodynamics were studied in 101 patients with acute myocardial infarction complicated by shock or left heart failure. 59 had anterior myocardial infarction (AMI); 42 had inferior myocardial infarction (IMI). Data were processed by univariate analysis and correspondence analysis. AMIs and IMIs were significantly different on conduction disturbances, heart rate, left ventricular filling pressure, mean pulmonary artery pressure and right ventricular function indices. Both patients and parameters were projected on the most meaningful factorial plane generated by correspondence analysis. This two-dimensional graphical representation showed that all the information was roughly distributed along the 2 orthogonal axes defining this plane. Survivors and nonsurvivors were fairly well separated along the first factorial axis (prognostic axis) which was highly correlated with both outcome and left ventricular function parameters. AMIs and IMIs were grossly separated along the second factorial axis (topographical axis) which was rather well correlated with location and right ventricular function parameters. These studies suggest that AMI and IMI hemodynamic profiles are modulated by the presence or absence of right ventricular dysfunction. Moreover right ventricular dysfunction may be held responsible of some lack of information about left ventricular function status.", "contents": "Relationships between hemodynamic profiles and topography of acute myocardial infarction. Initial hemodynamics were studied in 101 patients with acute myocardial infarction complicated by shock or left heart failure. 59 had anterior myocardial infarction (AMI); 42 had inferior myocardial infarction (IMI). Data were processed by univariate analysis and correspondence analysis. AMIs and IMIs were significantly different on conduction disturbances, heart rate, left ventricular filling pressure, mean pulmonary artery pressure and right ventricular function indices. Both patients and parameters were projected on the most meaningful factorial plane generated by correspondence analysis. This two-dimensional graphical representation showed that all the information was roughly distributed along the 2 orthogonal axes defining this plane. Survivors and nonsurvivors were fairly well separated along the first factorial axis (prognostic axis) which was highly correlated with both outcome and left ventricular function parameters. AMIs and IMIs were grossly separated along the second factorial axis (topographical axis) which was rather well correlated with location and right ventricular function parameters. These studies suggest that AMI and IMI hemodynamic profiles are modulated by the presence or absence of right ventricular dysfunction. Moreover right ventricular dysfunction may be held responsible of some lack of information about left ventricular function status."}
{"id": "PMID:467482", "title": "Comparative effects of nifedipine, verapamil, isosorbide dinitrate and propranolol on exercise-induced angina pectoris.", "content": "According to the experimental model of a 5 X 5 Latin square, 5 treatments were studied single blind in 5 patients, affected by stable-effort angina, by means of exercise tests. In the period of maximal supposed effect the following treatments were investigated: placebo (P), 1 tablet, orally; isosorbide dinitrate (ISDN), 5 mg, sublingually; propranolol (Pr), 40 mg, orally; nifedipine (N), 10 mg, orally; verapamil (V), 160 mg, orally. Placebo, compared with its own control tests, did not change any of the examined parameters. Comparison of the 'active' treatments with P showed the following results. All the treatments increased the duration of work before ECG positivity appearances. An increase in duration of work and total work performed before angina was seen after administration of ISIDN, N and V; the improvement observed after treatment with Pr was not significant. Comparison of treatments showed that work performance before angina was the same after administration of ISDN, N and V; these treatments were more effective than that with 40 mg Pr. Duration of work before ECG positivity was significantly longer after ISDN and N than after Pr. The changes in heart rate, maximal arterial pressure, ejection time index and triple product confirmed the activity of the administered doses. According to the observed effects on exercise tolerance, in comparison with P the same level of work was performed with the same triple product after Pr, and with lower triple products after ISDN, N and V.", "contents": "Comparative effects of nifedipine, verapamil, isosorbide dinitrate and propranolol on exercise-induced angina pectoris. According to the experimental model of a 5 X 5 Latin square, 5 treatments were studied single blind in 5 patients, affected by stable-effort angina, by means of exercise tests. In the period of maximal supposed effect the following treatments were investigated: placebo (P), 1 tablet, orally; isosorbide dinitrate (ISDN), 5 mg, sublingually; propranolol (Pr), 40 mg, orally; nifedipine (N), 10 mg, orally; verapamil (V), 160 mg, orally. Placebo, compared with its own control tests, did not change any of the examined parameters. Comparison of the 'active' treatments with P showed the following results. All the treatments increased the duration of work before ECG positivity appearances. An increase in duration of work and total work performed before angina was seen after administration of ISIDN, N and V; the improvement observed after treatment with Pr was not significant. Comparison of treatments showed that work performance before angina was the same after administration of ISDN, N and V; these treatments were more effective than that with 40 mg Pr. Duration of work before ECG positivity was significantly longer after ISDN and N than after Pr. The changes in heart rate, maximal arterial pressure, ejection time index and triple product confirmed the activity of the administered doses. According to the observed effects on exercise tolerance, in comparison with P the same level of work was performed with the same triple product after Pr, and with lower triple products after ISDN, N and V."}
{"id": "PMID:467483", "title": "Myocardial ischemia. Relationship between local flow, function and ST-segment elevation.", "content": "The significance of reductions in local myocardial flow on mechanical function and intramural electrocardiograms (ECG) was studied in anesthetized open-chest dogs. Local dimensional changes in the anterior wall of the left ventricle were recorded by ultrasonic technique. By platinum electrodes in the same region, both intramural ECG and hydrogen tension were recorded. Local flow was calculated from hydrogen desaturation curves. At approx. 25% flow reduction (constriction of the left anterior descending coronary artery (LAD)) local enddiastolic dilation and reduced systolic shortening appeared. Significant ST-segment elevation first appeared with flow reduction of 50%. After complete LAD-occlusions, enddiastolic dilation appeared within 20 sec, ST-segment elevation 40 sec later. It is concluded that mechanical and electrical events can be dissociated during acute myocardial ischemia: enddiastolic dilation and reduced systolic shortening are more sensitive indicators of moderate reductions in myocardial tissue flow than ST-segment elevation.", "contents": "Myocardial ischemia. Relationship between local flow, function and ST-segment elevation. The significance of reductions in local myocardial flow on mechanical function and intramural electrocardiograms (ECG) was studied in anesthetized open-chest dogs. Local dimensional changes in the anterior wall of the left ventricle were recorded by ultrasonic technique. By platinum electrodes in the same region, both intramural ECG and hydrogen tension were recorded. Local flow was calculated from hydrogen desaturation curves. At approx. 25% flow reduction (constriction of the left anterior descending coronary artery (LAD)) local enddiastolic dilation and reduced systolic shortening appeared. Significant ST-segment elevation first appeared with flow reduction of 50%. After complete LAD-occlusions, enddiastolic dilation appeared within 20 sec, ST-segment elevation 40 sec later. It is concluded that mechanical and electrical events can be dissociated during acute myocardial ischemia: enddiastolic dilation and reduced systolic shortening are more sensitive indicators of moderate reductions in myocardial tissue flow than ST-segment elevation."}
{"id": "PMID:467484", "title": "Failure to recognize a His bundle potential in complete atrioventricular block.", "content": "This report concerns a patient with complete heart block, in whom electrophysiological studies showed at times an escape rhythm with narrow QRS complexes preceded by His potentials with normal HV intervals (35--40 msec) and at other times an escape rhythm of similar rate, having wide QRS complexes of left bundle branch block configuration with no preceding His bundle activity. Complexes intermediate in width and configuration and preceded by His potentials with an HV interval inversely proportional to QRS width were also recorded. These observations are explained by a site of block proximal to the His bundle and competition between two pacemaker foci having similar discharge rates, one situated in the junctional region below the site of block and the other more distally in the right bundle branch or right ventricle. It is proposed that the combination of a proximal site of block and a distally situated dominant pacemaker may be a common reason for failure to record a His potential in patients with complete heart block.", "contents": "Failure to recognize a His bundle potential in complete atrioventricular block. This report concerns a patient with complete heart block, in whom electrophysiological studies showed at times an escape rhythm with narrow QRS complexes preceded by His potentials with normal HV intervals (35--40 msec) and at other times an escape rhythm of similar rate, having wide QRS complexes of left bundle branch block configuration with no preceding His bundle activity. Complexes intermediate in width and configuration and preceded by His potentials with an HV interval inversely proportional to QRS width were also recorded. These observations are explained by a site of block proximal to the His bundle and competition between two pacemaker foci having similar discharge rates, one situated in the junctional region below the site of block and the other more distally in the right bundle branch or right ventricle. It is proposed that the combination of a proximal site of block and a distally situated dominant pacemaker may be a common reason for failure to record a His potential in patients with complete heart block."}
{"id": "PMID:467486", "title": "Ectopic ventricular tachycardia in association with a concealed accessory pathway.", "content": "Ventricular tachycardia may result in retrograde atrial depolarization. In all previous examples studied by intracardiac recording techniques retrograde conduction to the atria has been thought to occur via the normal conduction system. In the patient reported here, 1 : 1 retrograde conduction with atrial preexcitation during ventricular tachycardia indicated the presence of a concealed accessory pathway. This was confirmed by the phenomenon of exact atrial capture. The achievement of atrioventricular dissociation during tachycardia by atrial overdrive pacing excluded a reentrant tachycardia involving such a pathway. The widely variable H--V interval in the presence of a constant V--A interval during tachycardia suggested anterograde His bundle depolarization following atrial activation over the accessory pathway. This hypothesis was supported by the long V--H interval during tachycardia and the relationship of the ventricular, atrial and His bundle electrograms of the first and least beat of the tachycardia. This report illustrates the value of careful electrophysiological investigation in evaluating the relationship of an accessory pathway to the tachycardia mechanism.", "contents": "Ectopic ventricular tachycardia in association with a concealed accessory pathway. Ventricular tachycardia may result in retrograde atrial depolarization. In all previous examples studied by intracardiac recording techniques retrograde conduction to the atria has been thought to occur via the normal conduction system. In the patient reported here, 1 : 1 retrograde conduction with atrial preexcitation during ventricular tachycardia indicated the presence of a concealed accessory pathway. This was confirmed by the phenomenon of exact atrial capture. The achievement of atrioventricular dissociation during tachycardia by atrial overdrive pacing excluded a reentrant tachycardia involving such a pathway. The widely variable H--V interval in the presence of a constant V--A interval during tachycardia suggested anterograde His bundle depolarization following atrial activation over the accessory pathway. This hypothesis was supported by the long V--H interval during tachycardia and the relationship of the ventricular, atrial and His bundle electrograms of the first and least beat of the tachycardia. This report illustrates the value of careful electrophysiological investigation in evaluating the relationship of an accessory pathway to the tachycardia mechanism."}
{"id": "PMID:467488", "title": "Echocardiographic diagnosis of congenital left ventricular - right atrial communication.", "content": "2 patients, aged 8 mth and 48 yr, referred for a clinical diagnosis of ventricular septal defect, were studied by ultrasound. In both a fluttering and an anterior bowing of the systolic segment of the tricuspid valve was found, consistent with a left ventricular--right atrial communication. Diagnosis was confirmed by angiography and open-heart surgery, showing in 1 patient a supravalvular defect, and an infravalvular one in the other. The postoperative echocardiograms of the tricuspid valve were normal. Ultrasound appears to be a safe and simple method in detecting this rare cardiac abnormality.", "contents": "Echocardiographic diagnosis of congenital left ventricular - right atrial communication. 2 patients, aged 8 mth and 48 yr, referred for a clinical diagnosis of ventricular septal defect, were studied by ultrasound. In both a fluttering and an anterior bowing of the systolic segment of the tricuspid valve was found, consistent with a left ventricular--right atrial communication. Diagnosis was confirmed by angiography and open-heart surgery, showing in 1 patient a supravalvular defect, and an infravalvular one in the other. The postoperative echocardiograms of the tricuspid valve were normal. Ultrasound appears to be a safe and simple method in detecting this rare cardiac abnormality."}
{"id": "PMID:467489", "title": "Antibody-dependent cellular cytotoxicity against tumor cells. I. Cultivated bone marrow-derived macrophages kill tumor targets.", "content": "Mouse bone marrow cells are cultivated in a liquid culture system in the presence of fibroblast conditioned medium. Under these conditions, proliferation of macrophage and granulocyte precursor cells is induced. Cells of a 5-day-old culture are shown to act as cytotoxic effector cells against tumor targets such as P815, E14, YAC and L5178Y. The effector cell is of macrophage origin since it is susceptible to treatment with the alloantiserum Mph-1.2 plus complement. The kinetics of the reaction resembles the kinetics for killer (K) lymphocyte lysis. In contrast to bone marrow cells, peritoneal macrophages do not show cytotoxic activity against antibody-coated tumor targets although they are susceptible to activation to cytotoxicity by lymphokines. The possible relationship of bone marrow effector cells and K lymphocytes is discussed.", "contents": "Antibody-dependent cellular cytotoxicity against tumor cells. I. Cultivated bone marrow-derived macrophages kill tumor targets. Mouse bone marrow cells are cultivated in a liquid culture system in the presence of fibroblast conditioned medium. Under these conditions, proliferation of macrophage and granulocyte precursor cells is induced. Cells of a 5-day-old culture are shown to act as cytotoxic effector cells against tumor targets such as P815, E14, YAC and L5178Y. The effector cell is of macrophage origin since it is susceptible to treatment with the alloantiserum Mph-1.2 plus complement. The kinetics of the reaction resembles the kinetics for killer (K) lymphocyte lysis. In contrast to bone marrow cells, peritoneal macrophages do not show cytotoxic activity against antibody-coated tumor targets although they are susceptible to activation to cytotoxicity by lymphokines. The possible relationship of bone marrow effector cells and K lymphocytes is discussed."}
{"id": "PMID:467490", "title": "Antibody-dependent cellular cytotoxicity against tumor cells. II. The promonocyte identified as effector cell.", "content": "Macrophage precursor cells were cultured from the bone marrow of mice in a liquid culture system in the presence of conditioned medium. To separate their different maturation stages, they were passed through a discontinuous Ficoll density gradient, treated with iron plus magnet or passed through glass bead columns. The different maturation stages have been tested for their function in antibody-dependent cellular cytotoxicity (ADCC) against tumor target cells and in lymphokine-induced macrophage-mediated cytotoxicity. It is shown that the promonocyte, a nonadherent, nonphagocytic precursor cell, is a highly potent cytotoxic effector cell against antibody-coated tumor targets but is totally inactive as an effector cell in lymphokine-induced macrophage-mediated cytotoxicity. In contrast, in the lymphokine-induced macrophage-mediated cytotoxicity the cytotoxic effector cell is a mature macrophage. Thus, ADCC seems to be a function of the macrophage precursor promonocytes, whereas lymphokine-induced cytotoxicity is performed by mature macrophages. The relationship of promonocytes and killer (k) cells in ADCC against tumor targets is discussed.", "contents": "Antibody-dependent cellular cytotoxicity against tumor cells. II. The promonocyte identified as effector cell. Macrophage precursor cells were cultured from the bone marrow of mice in a liquid culture system in the presence of conditioned medium. To separate their different maturation stages, they were passed through a discontinuous Ficoll density gradient, treated with iron plus magnet or passed through glass bead columns. The different maturation stages have been tested for their function in antibody-dependent cellular cytotoxicity (ADCC) against tumor target cells and in lymphokine-induced macrophage-mediated cytotoxicity. It is shown that the promonocyte, a nonadherent, nonphagocytic precursor cell, is a highly potent cytotoxic effector cell against antibody-coated tumor targets but is totally inactive as an effector cell in lymphokine-induced macrophage-mediated cytotoxicity. In contrast, in the lymphokine-induced macrophage-mediated cytotoxicity the cytotoxic effector cell is a mature macrophage. Thus, ADCC seems to be a function of the macrophage precursor promonocytes, whereas lymphokine-induced cytotoxicity is performed by mature macrophages. The relationship of promonocytes and killer (k) cells in ADCC against tumor targets is discussed."}
{"id": "PMID:467491", "title": "The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells.", "content": "The cellular distribution and the differential expression of HLA on cell suspensions and tissue sections has been investigated using the monoclonal antibody W6-32, which reacts with the high molecular weight chain of the major histocompatibility antigen. Lymphocytes and platelets, as assessed by autoradiographic and immunoperoxidase labeling, were the most densely labeled cells. Myeloid precursors showed more labeling than mature neutrophils. Electron microscopic immunoperoxidase labeling showed a continuous distribution of HLA antigen on lymphoid and myeloid cell membranes. Erythroid precursors (including reticulocytes), although very weakly labeled, were clearly positive, in comparison with mature erythrocytes. In the thymus, HLA-negative, thymocyte antigen-positive cells (85%) can be distinguished from HLA-positive, thymocyte antigen-negative cells (15%). By using immunofluorescence techniques on tissue sections, the former cells were shown to be cortical thymocytes and the latter medullary cells.", "contents": "The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells. The cellular distribution and the differential expression of HLA on cell suspensions and tissue sections has been investigated using the monoclonal antibody W6-32, which reacts with the high molecular weight chain of the major histocompatibility antigen. Lymphocytes and platelets, as assessed by autoradiographic and immunoperoxidase labeling, were the most densely labeled cells. Myeloid precursors showed more labeling than mature neutrophils. Electron microscopic immunoperoxidase labeling showed a continuous distribution of HLA antigen on lymphoid and myeloid cell membranes. Erythroid precursors (including reticulocytes), although very weakly labeled, were clearly positive, in comparison with mature erythrocytes. In the thymus, HLA-negative, thymocyte antigen-positive cells (85%) can be distinguished from HLA-positive, thymocyte antigen-negative cells (15%). By using immunofluorescence techniques on tissue sections, the former cells were shown to be cortical thymocytes and the latter medullary cells."}
{"id": "PMID:467492", "title": "A functional comparison of tumor cell killing by activated macrophages and natural killer cells.", "content": "This report compares the sensitivity of 17 tumor cell lines to cytolysis mediated by natural killer (NK) cells or by activated, bone marrow-derived macrophages (AM) from 15 inbred mouse strains. Some tumor cell lines, notably P815, were highly sensitive to AM-mediated lysis but almost completely insensitive to NK cells, whereas other cell lines were lysed by NK cells but not AM. In a genotype survey, some low-responder strains in the NK system, such as A/Sn, were high responders in the AM system, and conversely, one intermediate to high-responder strain (C3H/HeJ) in the NK system was a low responder in AM-mediated cytolysis. In addition, macrophage cytotoxicity factor was necessary to activate macrophages, but this lymphokine did not augment NK activity. Furthermore, the NK population did not contain pre-activated macrophages since pre-activated cells were removed on glass bead columns or by iron carbonyl and a magnet; treatments which have been previously shown not to affect NK cells. These results suggest that NK cells are distinct from AM in physical characteristics, target selectivity, genotype distribution and the mechanism of cytolysis.", "contents": "A functional comparison of tumor cell killing by activated macrophages and natural killer cells. This report compares the sensitivity of 17 tumor cell lines to cytolysis mediated by natural killer (NK) cells or by activated, bone marrow-derived macrophages (AM) from 15 inbred mouse strains. Some tumor cell lines, notably P815, were highly sensitive to AM-mediated lysis but almost completely insensitive to NK cells, whereas other cell lines were lysed by NK cells but not AM. In a genotype survey, some low-responder strains in the NK system, such as A/Sn, were high responders in the AM system, and conversely, one intermediate to high-responder strain (C3H/HeJ) in the NK system was a low responder in AM-mediated cytolysis. In addition, macrophage cytotoxicity factor was necessary to activate macrophages, but this lymphokine did not augment NK activity. Furthermore, the NK population did not contain pre-activated macrophages since pre-activated cells were removed on glass bead columns or by iron carbonyl and a magnet; treatments which have been previously shown not to affect NK cells. These results suggest that NK cells are distinct from AM in physical characteristics, target selectivity, genotype distribution and the mechanism of cytolysis."}
{"id": "PMID:467493", "title": "Studies on the inhibitory action of somatostatin in the electrically stimulated rat vas deferens.", "content": "Somatostatin (SS) inhibits in a dose-dependent manner electrically evoked contractions in the rat vas deferens. This action was not modified by yohimbine, naloxone or a mixture of antagonists containing atropine, phentolamine, methysergide, burimamide, propranolol and indomethacin, but was markedly potentiated by reducing the Ca2+ concentration of the medium from 2.5 to 1.25 mM and greatly inhibited when increasing the Ca2+ concentration of the medium from 2.5 to 5.0 mM. Clonidine (CLO), but not beta-endorphin (ENDO) was affected similarly to SS by changing the Ca2+ concentration of the medium. The contractile effect of norepinephrine in unstimulated rat vas deferens was not altered by SS. These results were taken as an indication that SS produces its inhibitory action in the rat vas deferens by interacting with specific SS and its receptors presumably located in the cell membranes of adrenergic nerve terminals. The interaction between SS and its receptors may provoke a decreased diffusion of Ca2+ ions into the nerve terminals and/or a decreased mobilisation of Ca2+ ions from intraneuronal stores thus leading to a reduction in electrically evoked release of norepinephrine.", "contents": "Studies on the inhibitory action of somatostatin in the electrically stimulated rat vas deferens. Somatostatin (SS) inhibits in a dose-dependent manner electrically evoked contractions in the rat vas deferens. This action was not modified by yohimbine, naloxone or a mixture of antagonists containing atropine, phentolamine, methysergide, burimamide, propranolol and indomethacin, but was markedly potentiated by reducing the Ca2+ concentration of the medium from 2.5 to 1.25 mM and greatly inhibited when increasing the Ca2+ concentration of the medium from 2.5 to 5.0 mM. Clonidine (CLO), but not beta-endorphin (ENDO) was affected similarly to SS by changing the Ca2+ concentration of the medium. The contractile effect of norepinephrine in unstimulated rat vas deferens was not altered by SS. These results were taken as an indication that SS produces its inhibitory action in the rat vas deferens by interacting with specific SS and its receptors presumably located in the cell membranes of adrenergic nerve terminals. The interaction between SS and its receptors may provoke a decreased diffusion of Ca2+ ions into the nerve terminals and/or a decreased mobilisation of Ca2+ ions from intraneuronal stores thus leading to a reduction in electrically evoked release of norepinephrine."}
{"id": "PMID:467494", "title": "Effect of estradiol and progesterone on the isometric tension developed by longitudinal and circular isthmus of rabbit fallopian tube.", "content": "The effect of estradiol and progesterone on the isometric tension developed by longitudinal and circular muscle of isthmus of rabbit fallopian tube to various agonists was studied. The isometric tension developed was reduced by about 50% under progesterone dominance as compared to estrogen dominance. This inhibition was of non-specific nature and did not involve adrenergic receptors. The non-specific effect of progesterone may have a role in regulating the entry of ova into the isthmus after their initial retention at the ampullary--isthmic junction.", "contents": "Effect of estradiol and progesterone on the isometric tension developed by longitudinal and circular isthmus of rabbit fallopian tube. The effect of estradiol and progesterone on the isometric tension developed by longitudinal and circular muscle of isthmus of rabbit fallopian tube to various agonists was studied. The isometric tension developed was reduced by about 50% under progesterone dominance as compared to estrogen dominance. This inhibition was of non-specific nature and did not involve adrenergic receptors. The non-specific effect of progesterone may have a role in regulating the entry of ova into the isthmus after their initial retention at the ampullary--isthmic junction."}
{"id": "PMID:467495", "title": "The effect of neurotensin on the plasma cholesterol levels in the rat.", "content": "The effect of the hypothalamic tridecapeptide neurotensin (NT) on the plasma cholesterol levels was studied in the rat. 15 min after the intravenous administration of 1.25 pmol/100 g body weight NT, the concentration of the total plasma cholesterol was increased about 25% above the control values (P less than 0.001) and remained at about the same level 30 min after the injection. The hypercholesterolemic response to the intravenous administration of NT increased dose dependently (12.5 and 125 pmol). Adrenalectomy and hypophysectomy did not modify the hypercholesterolemic effect of NT. The data suggest that NT may have a role in cholesterol transport.", "contents": "The effect of neurotensin on the plasma cholesterol levels in the rat. The effect of the hypothalamic tridecapeptide neurotensin (NT) on the plasma cholesterol levels was studied in the rat. 15 min after the intravenous administration of 1.25 pmol/100 g body weight NT, the concentration of the total plasma cholesterol was increased about 25% above the control values (P less than 0.001) and remained at about the same level 30 min after the injection. The hypercholesterolemic response to the intravenous administration of NT increased dose dependently (12.5 and 125 pmol). Adrenalectomy and hypophysectomy did not modify the hypercholesterolemic effect of NT. The data suggest that NT may have a role in cholesterol transport."}
{"id": "PMID:467496", "title": "Evaluation of the anticholinesterase activity of metronidazole and misonidazole.", "content": "Contrary to an earlier report that metronidazole exhibited anticholinesterase activity on isolated organ preparations, a direct spectrophotometric assay was unable to demonstrate any significant inhibition of either true or pseudocholinesterase activity in the presence of this drug. The related nitroimidazole, misonidazole, was also found to be inactive when tested in this  system. This report demonstrates that the clinical neurotoxicity of these two compounds cannot be attributed to any direct effect on the cholinesterase enzymes.", "contents": "Evaluation of the anticholinesterase activity of metronidazole and misonidazole. Contrary to an earlier report that metronidazole exhibited anticholinesterase activity on isolated organ preparations, a direct spectrophotometric assay was unable to demonstrate any significant inhibition of either true or pseudocholinesterase activity in the presence of this drug. The related nitroimidazole, misonidazole, was also found to be inactive when tested in this  system. This report demonstrates that the clinical neurotoxicity of these two compounds cannot be attributed to any direct effect on the cholinesterase enzymes."}
{"id": "PMID:467497", "title": "An analysis of the stimulant effects of 5-hydroxytryptamine on isolated, blood-perfused rat heart.", "content": "In rat isolated hearts perfused at a fixed low rate, single injections of 5-hydroxytryptamine (5-HT) (0.1--3 microgram) into the coronary perfusion produced dose-dependent increases in left ventricular (LV) dP/dt max and perfusion pressure (PP), but no clear changes in heart rate. The increases in the LVdP/dt max and PP were not significantly affected by treatment with propranolol, while they were abolished by methysergide. The present study indicates that the positive inotropic and vasoconstrictor responses of the rat heart to 5-HT are not mediated by endogenous catecholamine release, but are induced by a direct action on 5-HT receptors.", "contents": "An analysis of the stimulant effects of 5-hydroxytryptamine on isolated, blood-perfused rat heart. In rat isolated hearts perfused at a fixed low rate, single injections of 5-hydroxytryptamine (5-HT) (0.1--3 microgram) into the coronary perfusion produced dose-dependent increases in left ventricular (LV) dP/dt max and perfusion pressure (PP), but no clear changes in heart rate. The increases in the LVdP/dt max and PP were not significantly affected by treatment with propranolol, while they were abolished by methysergide. The present study indicates that the positive inotropic and vasoconstrictor responses of the rat heart to 5-HT are not mediated by endogenous catecholamine release, but are induced by a direct action on 5-HT receptors."}
{"id": "PMID:467498", "title": "Release of beta-endorphin from rat hypothalamus in vitro.", "content": "The rate of release of beta-endorphin-like immunoreactivity (beta-EI) from rat hypothalamic slices was increased 3- to 4-fold over the spontaneous release during exposure to a depolarizing concentration of potassium ions. This augmented outflow was abolished in the absence of calcium. The amount of beta-EI released from slices exposed to a second pulse of potassium was reduced by approximately 50% compared to that in the first. 70% of the potassium-induced released immunoreactive material migrated on a calibrated Sephadex G-50 column like synthetic human beta-endorphin. These findings are discussed in terms of a neuronal release of beta-endorphin and may be taken as supportive evidence for a neurotransmitter and/or neurohormonal role of beta-endorphin in the hypothalamus.", "contents": "Release of beta-endorphin from rat hypothalamus in vitro. The rate of release of beta-endorphin-like immunoreactivity (beta-EI) from rat hypothalamic slices was increased 3- to 4-fold over the spontaneous release during exposure to a depolarizing concentration of potassium ions. This augmented outflow was abolished in the absence of calcium. The amount of beta-EI released from slices exposed to a second pulse of potassium was reduced by approximately 50% compared to that in the first. 70% of the potassium-induced released immunoreactive material migrated on a calibrated Sephadex G-50 column like synthetic human beta-endorphin. These findings are discussed in terms of a neuronal release of beta-endorphin and may be taken as supportive evidence for a neurotransmitter and/or neurohormonal role of beta-endorphin in the hypothalamus."}
{"id": "PMID:467501", "title": "K-stimulated release of leu- and met-enkephalin from rat striatal slices: lack of effect of morphine and naloxone.", "content": "The release of leu- and met-enkephalin from rat striatal slices was determined by superfusing the slices in vitro and running the superfusates directly over columns of Amberlite XAD-2 from which the peptides were eluted with methanol and measured by radioimmunoassay. Depolarization by high K concentrations increased the release of both pentapeptides many fold; the degree of increase, however, depended in part on the length of time chosen for the stimulation period, suggesting that the stimulation effect was very short lived. The stimulated release of both peptides (but not the resting release) was at least partially dependent on Ca in the medium and was totally inhibited by high Mg concentrations. Selected concentrations of naloxone and morphine in the superfusing medium had no effect on the resting or stimulated release of the peptides. The results support the hypothesis that these peptides serve as neurotransmitters in the striatum, but autoregulation of their release by morphine and naloxone could not be demonstrated.", "contents": "K-stimulated release of leu- and met-enkephalin from rat striatal slices: lack of effect of morphine and naloxone. The release of leu- and met-enkephalin from rat striatal slices was determined by superfusing the slices in vitro and running the superfusates directly over columns of Amberlite XAD-2 from which the peptides were eluted with methanol and measured by radioimmunoassay. Depolarization by high K concentrations increased the release of both pentapeptides many fold; the degree of increase, however, depended in part on the length of time chosen for the stimulation period, suggesting that the stimulation effect was very short lived. The stimulated release of both peptides (but not the resting release) was at least partially dependent on Ca in the medium and was totally inhibited by high Mg concentrations. Selected concentrations of naloxone and morphine in the superfusing medium had no effect on the resting or stimulated release of the peptides. The results support the hypothesis that these peptides serve as neurotransmitters in the striatum, but autoregulation of their release by morphine and naloxone could not be demonstrated."}
{"id": "PMID:467502", "title": "Effect of prostaglandin E1 and indomethacin on responses of longitudinal muscle of guinea-pig ileum to cholecystokinin.", "content": "The effect of prostaglandin E1 (PGE1) and indomethacin (IND) cholecystokinin (CCK)-induced contractions of guinea-pig isolated ileum longitudinal muscle were studied. PGE1 (2.8--28 nM) consistently and dose dependently increased the contractions evoked by CCK (indirect muscle stimulation) or by ACh. IND (2.7 microM) decreased the contractions to both compounds and this was reversed with 2.8--7 nM PGE1. Pretreatment of the preparations with phentolamine (2.6 microM) or pretreatment of the animals with reserpine (2 mg/kg i.p. 24 h before killing) did not affect PGE1 potentiation or IND inhibition of CCK-induced contractions. The results indicated that PGE1 potentiated CCK-induced contractions of the longitudinal muscle of guinea-pig ileum by increasing the response to released ACh. Experiments with IND suggested that endogenous PGs may modulate the effect of CCK or related gastrointestinal hormones.", "contents": "Effect of prostaglandin E1 and indomethacin on responses of longitudinal muscle of guinea-pig ileum to cholecystokinin. The effect of prostaglandin E1 (PGE1) and indomethacin (IND) cholecystokinin (CCK)-induced contractions of guinea-pig isolated ileum longitudinal muscle were studied. PGE1 (2.8--28 nM) consistently and dose dependently increased the contractions evoked by CCK (indirect muscle stimulation) or by ACh. IND (2.7 microM) decreased the contractions to both compounds and this was reversed with 2.8--7 nM PGE1. Pretreatment of the preparations with phentolamine (2.6 microM) or pretreatment of the animals with reserpine (2 mg/kg i.p. 24 h before killing) did not affect PGE1 potentiation or IND inhibition of CCK-induced contractions. The results indicated that PGE1 potentiated CCK-induced contractions of the longitudinal muscle of guinea-pig ileum by increasing the response to released ACh. Experiments with IND suggested that endogenous PGs may modulate the effect of CCK or related gastrointestinal hormones."}
{"id": "PMID:467503", "title": "Interaction of sulfpride and ergot derivatives on rat brain DOPAC concentration and prolactin secretion in vivo.", "content": "Sulpiride, which differs from classical neuroleptics by not producing major extrapyramidal side effects, is a potent antiemetic agent and stimulates prolactin secretion in both laboratory animals and man. In parallel it increases dopamine synthesis in both striatum and nucleus accumbens. Bromocriptine and metergoline are two effective agents in suppressing prolactin release and postulated to stimulate dopamine receptors. The interactions of these two ergot derivatives with sulpiride have been investigated on prolactin release and on striatal and limbic DOPAC accumulation. Bromocriptine at all doses tested was able to suppress the increased in vivo prolactin secretion observed after sulpiride administration. Metergoline antagonized the sulpiride-induced prolactin increase only at low doses; on the contrary higher doses potentiated it. High concentrations of bromocriptine suppressed the sulpiride-induced increased of DOPAC levels in striatum and n. accumbens, while metergoline potentiated the sulpiride-induced accumulation of brain DOPAC.", "contents": "Interaction of sulfpride and ergot derivatives on rat brain DOPAC concentration and prolactin secretion in vivo. Sulpiride, which differs from classical neuroleptics by not producing major extrapyramidal side effects, is a potent antiemetic agent and stimulates prolactin secretion in both laboratory animals and man. In parallel it increases dopamine synthesis in both striatum and nucleus accumbens. Bromocriptine and metergoline are two effective agents in suppressing prolactin release and postulated to stimulate dopamine receptors. The interactions of these two ergot derivatives with sulpiride have been investigated on prolactin release and on striatal and limbic DOPAC accumulation. Bromocriptine at all doses tested was able to suppress the increased in vivo prolactin secretion observed after sulpiride administration. Metergoline antagonized the sulpiride-induced prolactin increase only at low doses; on the contrary higher doses potentiated it. High concentrations of bromocriptine suppressed the sulpiride-induced increased of DOPAC levels in striatum and n. accumbens, while metergoline potentiated the sulpiride-induced accumulation of brain DOPAC."}
{"id": "PMID:467504", "title": "Effect of 6-hydroxydopamine treatment on TSH secretion in basal and cold-stimulated conditions in the rat.", "content": "6-Hydroxydopamine (6-OHDA) (two doses of 200 micrograms each, administered intraventricularly at a 48 h interval) caused a marked decrease of hypothalamic noradrenaline content and blocked the TSH rise elicited by cold exposure. Clonidine (0.4 mg/kg i.p.), a noradrenaline receptor agonist, was able to reverse the 6-OHDA of cold-induced TSH surge. The plasma TSH levels after cold stress in rats treated with 6-OHDA + clonidine were significantly higher than in vehicle + clonidine-injected animals, thus suggesting the presence of noradrenaline receptor supersensitivity in 6-OHDA-pretreated rats. 6-OHDA did not modify the basal concentrations of TSH but the administration of clonidine to 6-OHDA-injected animals caused a significant increase in thyrotropin secretion when compared with the vehicle + clonidine group.", "contents": "Effect of 6-hydroxydopamine treatment on TSH secretion in basal and cold-stimulated conditions in the rat. 6-Hydroxydopamine (6-OHDA) (two doses of 200 micrograms each, administered intraventricularly at a 48 h interval) caused a marked decrease of hypothalamic noradrenaline content and blocked the TSH rise elicited by cold exposure. Clonidine (0.4 mg/kg i.p.), a noradrenaline receptor agonist, was able to reverse the 6-OHDA of cold-induced TSH surge. The plasma TSH levels after cold stress in rats treated with 6-OHDA + clonidine were significantly higher than in vehicle + clonidine-injected animals, thus suggesting the presence of noradrenaline receptor supersensitivity in 6-OHDA-pretreated rats. 6-OHDA did not modify the basal concentrations of TSH but the administration of clonidine to 6-OHDA-injected animals caused a significant increase in thyrotropin secretion when compared with the vehicle + clonidine group."}
{"id": "PMID:467505", "title": "Effects of diethylcholine in two animal models of Parkinsonism tremors.", "content": "(2-Hydroxyethyl) methyldiethylammonium iodide (diethylcholine; DEC) was tested against trihexyphenidyl for its ability to block tremors in two animal models of Parkinsonism tremors. Both DEC (75 mg/kg) and trihexyphenidyl (10 mg/kg) antagonized physostigmine tremors in mice. Both drugs also blocked tremors in rats which received intracaudate injections of carbachol. DEC was more efficacious than trihexyphenidyl in the rat model. No dose-related inhibition of tremors was seen for trihexyphenidyl (5--20 mg/kg) but inhibition by DEC was dose-related (25--50 mg/kg). The ED50 for tremor inhibition in the rat model by DEC was 33 mg/kg. DEC was also shown to cross the blood-brain barrier in mice. The probable mechanism of action of DEC is discussed.", "contents": "Effects of diethylcholine in two animal models of Parkinsonism tremors. (2-Hydroxyethyl) methyldiethylammonium iodide (diethylcholine; DEC) was tested against trihexyphenidyl for its ability to block tremors in two animal models of Parkinsonism tremors. Both DEC (75 mg/kg) and trihexyphenidyl (10 mg/kg) antagonized physostigmine tremors in mice. Both drugs also blocked tremors in rats which received intracaudate injections of carbachol. DEC was more efficacious than trihexyphenidyl in the rat model. No dose-related inhibition of tremors was seen for trihexyphenidyl (5--20 mg/kg) but inhibition by DEC was dose-related (25--50 mg/kg). The ED50 for tremor inhibition in the rat model by DEC was 33 mg/kg. DEC was also shown to cross the blood-brain barrier in mice. The probable mechanism of action of DEC is discussed."}
{"id": "PMID:467506", "title": "Angiotensin II facilitates the potassium-evoked release of 3H-noradrenaline from the rabbit hypothalamus.", "content": "Antiotensin II facilitated in a concentration-dependent manner the potassium-evoked 3H-noradrenaline over-flow from the rabbit hypothalamus. This effect which is probably mediated through presynaptic angiotensin facilitatory receptors on noradrenergic nerve terminals was blocked by the specific angiotensin receptor antagonist, saralasin. These results demonstrate that angiotensin II also facilitates the stimulation-evoked release of noradrenaline in the central nervous system.", "contents": "Angiotensin II facilitates the potassium-evoked release of 3H-noradrenaline from the rabbit hypothalamus. Antiotensin II facilitated in a concentration-dependent manner the potassium-evoked 3H-noradrenaline over-flow from the rabbit hypothalamus. This effect which is probably mediated through presynaptic angiotensin facilitatory receptors on noradrenergic nerve terminals was blocked by the specific angiotensin receptor antagonist, saralasin. These results demonstrate that angiotensin II also facilitates the stimulation-evoked release of noradrenaline in the central nervous system."}
{"id": "PMID:467511", "title": "An attempt to differentiate the effects of angiotensin II, substance P and bradykinin on the field-stimulated guinea-pig vas deferens.", "content": "The ability of the angiotensin antagonists 1-Sar,8-Ala-angiotensin II (saralasin) and 1-Sar,8-Leu-angiotensin II (sarleusin) and the bradykinin-potentiating peptide B (BPP) to modify the twitch-enhancing effect of angiotensin II, bradykinin, and substance P, was studied in the isolated field-stimulated guinea-pig vas deferens. The twitch-enhancing effect of angiotensin, bradykinin and substance P underwent tachyphylaxis which was strongest after angiotensin. Saralasin and sarleusin were without influence on the twitch response and antagonized the effect of angiotensin, but not that of bradykinin or substance P, respectively. The features of the antagonism to angiotensin were compatible with the notion that saralasin is a competitive and sarleusin a dual antagonist. BPP did modify either the twitch response or the effects of angiotensin, bradykinin, and substance P. In the non-stimulate vas, the contracting effect of noradrenaline did not undergo tachyphylaxis and was not mofified by angiotensin, bradykinin, or substance P. It is concluded that there exist in the guinea-pig vas specific peptide receptors, one of them having the properties of a \"typical\" angiotensin receptor.", "contents": "An attempt to differentiate the effects of angiotensin II, substance P and bradykinin on the field-stimulated guinea-pig vas deferens. The ability of the angiotensin antagonists 1-Sar,8-Ala-angiotensin II (saralasin) and 1-Sar,8-Leu-angiotensin II (sarleusin) and the bradykinin-potentiating peptide B (BPP) to modify the twitch-enhancing effect of angiotensin II, bradykinin, and substance P, was studied in the isolated field-stimulated guinea-pig vas deferens. The twitch-enhancing effect of angiotensin, bradykinin and substance P underwent tachyphylaxis which was strongest after angiotensin. Saralasin and sarleusin were without influence on the twitch response and antagonized the effect of angiotensin, but not that of bradykinin or substance P, respectively. The features of the antagonism to angiotensin were compatible with the notion that saralasin is a competitive and sarleusin a dual antagonist. BPP did modify either the twitch response or the effects of angiotensin, bradykinin, and substance P. In the non-stimulate vas, the contracting effect of noradrenaline did not undergo tachyphylaxis and was not mofified by angiotensin, bradykinin, or substance P. It is concluded that there exist in the guinea-pig vas specific peptide receptors, one of them having the properties of a \"typical\" angiotensin receptor."}
{"id": "PMID:467512", "title": "Effect of ergotamine on cranial arteriovenous shunting in experiments with constant flow perfusion.", "content": "The effect of the antimigraine drug, ergotamine, was studied on the distribution of flow throughout the cranial microcirculation of the cat, using microspheres suitable in size to separate capillary flow from flow through the arteriovenous anastomoses. A constant flow perfusion experiment was used in order to eliminate the secondary effect of ergotamine on the microcirculation through a reduction in carotid blood flow per se. Under the experimental conditions, ergotamine decreased in a dose-dependent way the fraction of perfusion flow shunted through the arteriovenous anastomoses. This mode of action of ergotamine sheds new light on the so-called migraine shunt theory.", "contents": "Effect of ergotamine on cranial arteriovenous shunting in experiments with constant flow perfusion. The effect of the antimigraine drug, ergotamine, was studied on the distribution of flow throughout the cranial microcirculation of the cat, using microspheres suitable in size to separate capillary flow from flow through the arteriovenous anastomoses. A constant flow perfusion experiment was used in order to eliminate the secondary effect of ergotamine on the microcirculation through a reduction in carotid blood flow per se. Under the experimental conditions, ergotamine decreased in a dose-dependent way the fraction of perfusion flow shunted through the arteriovenous anastomoses. This mode of action of ergotamine sheds new light on the so-called migraine shunt theory."}
{"id": "PMID:467513", "title": "Dopamine vascular actions of N-substituted derivatives of 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (ADTN).", "content": "N-ethyl, N-n-propyl, N-n-butyl, N,N-di-n-propyl and N-n-propyl-N-n-butyl derivatives of 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (ADTN) were screened for dopamine vascular agonist activity. Effects of intraarterial injections of the drugs on renal and femoral blood flow were measured in pentobarbital-anesthetized dogs before and after phenoxybenzamine (POB), 5--10 mg/kg. All DDTN derivatives were more potent vasoconstrictors than dopamine (DA) before POB. The di-substituted analogs were approximately 1/4 and the mono-substituted derivatives were about 1/16 as active as norepinephrine as vasoconstrictors. After POB the two di-substituted analogs were about 1/4 as active as DA in causing renal vasodilation. The three mono-substituted analogs produced relatively minor and inconsistent effects on renal blood flow, and were more than 100 times less effective than DA as vasodilators. The two di-substituted analogs markedly increased blood pressure with little effect on cardiac contractility in doses up to 32 micrograms/kg; whereas, the threshold dose of DA is 4--8 micrograms/kg. This and our previous results show that structure activity relationships of DA and ADTN, a semi-rigid analog of DA, are similar. However, the rigid analogs tend to be more potent vasoconstrictors than their flexible chain homologs.", "contents": "Dopamine vascular actions of N-substituted derivatives of 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (ADTN). N-ethyl, N-n-propyl, N-n-butyl, N,N-di-n-propyl and N-n-propyl-N-n-butyl derivatives of 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (ADTN) were screened for dopamine vascular agonist activity. Effects of intraarterial injections of the drugs on renal and femoral blood flow were measured in pentobarbital-anesthetized dogs before and after phenoxybenzamine (POB), 5--10 mg/kg. All DDTN derivatives were more potent vasoconstrictors than dopamine (DA) before POB. The di-substituted analogs were approximately 1/4 and the mono-substituted derivatives were about 1/16 as active as norepinephrine as vasoconstrictors. After POB the two di-substituted analogs were about 1/4 as active as DA in causing renal vasodilation. The three mono-substituted analogs produced relatively minor and inconsistent effects on renal blood flow, and were more than 100 times less effective than DA as vasodilators. The two di-substituted analogs markedly increased blood pressure with little effect on cardiac contractility in doses up to 32 micrograms/kg; whereas, the threshold dose of DA is 4--8 micrograms/kg. This and our previous results show that structure activity relationships of DA and ADTN, a semi-rigid analog of DA, are similar. However, the rigid analogs tend to be more potent vasoconstrictors than their flexible chain homologs."}
{"id": "PMID:467514", "title": "Actions of somatostatin on excitatory and inhibitory nerves in the intestine.", "content": "Isolated segments from the guinea-pig ileum and colon were used to investigate the sites of action of somatostatin on intestinal nerves and muscle. Somatostatin reduced the amplitude of the contraction caused by stimulation of cholinergic nerves in the ileum, without antagonizing receptors for acetylcholine on the muscle, which indicates that somatostatin inhibits the release of transmitter from the cholinergic nerves. Somatostatin caused the intestinal muscle to relax. The relaxation was blocked by tetrodotoxin but not by guanethidine, indicating that somatostatin stimulates enteric non-adrenergic inhibitory nerves. The threshold of concentration for these actions of somatostatin was low (2 to 6 X 10(-10) M). At higher concentrations, somatostatin caused a transient inhibition of peristaltic reflexes and it also stimulated cholinergic nerves. No evidence was obtained for a direct action of somatostatin on intestinal muscle. It is concluded that somatostatin is possibly a neurotransmitter in the intestine that is released at neuro-neuronal junctions and causes an inhibition of intestinal movements.", "contents": "Actions of somatostatin on excitatory and inhibitory nerves in the intestine. Isolated segments from the guinea-pig ileum and colon were used to investigate the sites of action of somatostatin on intestinal nerves and muscle. Somatostatin reduced the amplitude of the contraction caused by stimulation of cholinergic nerves in the ileum, without antagonizing receptors for acetylcholine on the muscle, which indicates that somatostatin inhibits the release of transmitter from the cholinergic nerves. Somatostatin caused the intestinal muscle to relax. The relaxation was blocked by tetrodotoxin but not by guanethidine, indicating that somatostatin stimulates enteric non-adrenergic inhibitory nerves. The threshold of concentration for these actions of somatostatin was low (2 to 6 X 10(-10) M). At higher concentrations, somatostatin caused a transient inhibition of peristaltic reflexes and it also stimulated cholinergic nerves. No evidence was obtained for a direct action of somatostatin on intestinal muscle. It is concluded that somatostatin is possibly a neurotransmitter in the intestine that is released at neuro-neuronal junctions and causes an inhibition of intestinal movements."}
{"id": "PMID:467515", "title": "Effect of cardiotonic polypeptide anthopleurin-A on canine Purkinje and ventricular muscle fibers.", "content": "Effects of Anthopleurin-A (AP-A, polypeptide from sea anemone) were studied on electrophysiological properties of isolated canine Purkinje and ventricular muscle fibers. Ap-A (in concentrations above 20 micrograms/l) produced a dose-dependent increase in action potential duration (APD) and the refractory period (RFP) in electrically driven Purkinje fibers, but had no effect on other parameters. Similar but less prominent change in APD and RFP was observed in ventricular muscle fibers. AP-A in high concentrations (200 micrograms/l or higher) did alter the spontaneous firing rate of Purkinje fibers. Since AP-A in low concentrations will increase the refractory period of conducting fibers without affecting a conduction velocity, it may abolish some re-entrant arrhythmias.", "contents": "Effect of cardiotonic polypeptide anthopleurin-A on canine Purkinje and ventricular muscle fibers. Effects of Anthopleurin-A (AP-A, polypeptide from sea anemone) were studied on electrophysiological properties of isolated canine Purkinje and ventricular muscle fibers. Ap-A (in concentrations above 20 micrograms/l) produced a dose-dependent increase in action potential duration (APD) and the refractory period (RFP) in electrically driven Purkinje fibers, but had no effect on other parameters. Similar but less prominent change in APD and RFP was observed in ventricular muscle fibers. AP-A in high concentrations (200 micrograms/l or higher) did alter the spontaneous firing rate of Purkinje fibers. Since AP-A in low concentrations will increase the refractory period of conducting fibers without affecting a conduction velocity, it may abolish some re-entrant arrhythmias."}
{"id": "PMID:467516", "title": "Tetrahydronorharmane modulates the depolarisation-induced efflux of 5-hydroxytryptamine and dopamine and is released by high potassium concentration from rat brain slices.", "content": "Tetrahydronorharmane (THN), a compound which occurs in man and in rats is supposed to act as a modulator of 5-hydroxytryptaminergic neurones. It is reported here that THN stimulated the potassium-induced release of the neurotransmitter from brain slices. This suggests enhancement of the efflux as a mechanism for the 5-hydroxytryptamine-like action of THN in pharmacological experiments. Furthermore, THN decreased the potassium-evoked efflux of (3H)-dopamine. This is consistent with the inhibition of dopaminergic neurones by THN as seen previously in various in vivo experiments. Finally it is demonstrated that a high concentration of potassium evokes the efflux of (14C)-THN from brain slices. This finding suggests that THN is taken up into neurones and exerts its modulating action on serotonergic neurones either inside or outside the cell.", "contents": "Tetrahydronorharmane modulates the depolarisation-induced efflux of 5-hydroxytryptamine and dopamine and is released by high potassium concentration from rat brain slices. Tetrahydronorharmane (THN), a compound which occurs in man and in rats is supposed to act as a modulator of 5-hydroxytryptaminergic neurones. It is reported here that THN stimulated the potassium-induced release of the neurotransmitter from brain slices. This suggests enhancement of the efflux as a mechanism for the 5-hydroxytryptamine-like action of THN in pharmacological experiments. Furthermore, THN decreased the potassium-evoked efflux of (3H)-dopamine. This is consistent with the inhibition of dopaminergic neurones by THN as seen previously in various in vivo experiments. Finally it is demonstrated that a high concentration of potassium evokes the efflux of (14C)-THN from brain slices. This finding suggests that THN is taken up into neurones and exerts its modulating action on serotonergic neurones either inside or outside the cell."}
{"id": "PMID:467517", "title": "Electrophysiological study of the action of neurotensin on the smooth muscle of the guinea-pig taenia coli.", "content": "The effects of neurotensin on the membrane potential and conductance, and on the tension of the guinea-pig taenia coli were investigated using the sucrose gap method. In preparations spontaneously active at 37 degrees C, neurotensin (0.5--10 nM) increased the frequency of spikes and induced a smooth muscle membrane depolarization which led to a block of spike discharges. These electrical events were accompanied by phasic and tonic contractions of the smooth muscle. At 20 degrees C the guinea-pig taenia coli exhibited little or no spontaneous activity. Neurotensin (50 nM) rapidly depolarized the smooth muscle membrane and increased its conductance. Concentration--response curves of neurotensin-induced depolarization and contraction gave an EC50 of 1.7 nM and 4.5 nM respectively, with a maximally effective concentration of peptide around 50 nM for both effects. Studies in modified or substituted salt solutions indicated that the neurotensin-induced increase in smooth muscle membrane conductance was primarily due to an increase in Na+ and Ca2+ conductances.", "contents": "Electrophysiological study of the action of neurotensin on the smooth muscle of the guinea-pig taenia coli. The effects of neurotensin on the membrane potential and conductance, and on the tension of the guinea-pig taenia coli were investigated using the sucrose gap method. In preparations spontaneously active at 37 degrees C, neurotensin (0.5--10 nM) increased the frequency of spikes and induced a smooth muscle membrane depolarization which led to a block of spike discharges. These electrical events were accompanied by phasic and tonic contractions of the smooth muscle. At 20 degrees C the guinea-pig taenia coli exhibited little or no spontaneous activity. Neurotensin (50 nM) rapidly depolarized the smooth muscle membrane and increased its conductance. Concentration--response curves of neurotensin-induced depolarization and contraction gave an EC50 of 1.7 nM and 4.5 nM respectively, with a maximally effective concentration of peptide around 50 nM for both effects. Studies in modified or substituted salt solutions indicated that the neurotensin-induced increase in smooth muscle membrane conductance was primarily due to an increase in Na+ and Ca2+ conductances."}
{"id": "PMID:467518", "title": "Studies on the protective effect of prostacyclin in acute myocardial ischemia.", "content": "The ability of prostacyclin (PGI2) to alter responses to acute myocardial ischemia was studied in open-chest, anesthetized cats. PGI2 was infused intravenously at 0.5 nmoles kg-1 min-1 in cats subjected to 5 h of myocardial ischemia by occlusion of the LAD coronary artery, and in sham-operated controls. GI2 infusion resulted in significantly decreased arterial blood pressure and inhibition of platelet aggregation. Coronary ligation resulted in significant S-T segment elevations lasting 5 h in vehicle-treated animals but only 1 h in cats with myocardial ischemia and receiving PGI2. At 5 h, cats with ischemia and given the vehicle showed S-T segment elevations significantly greater than the other two groups. Ischemic myocardium from vehicle-treated animals exhibited significantly less creatine phosphokinase (CPK) specific activity than normal tissue from the same hearts or myocardial tissue from the other two groups. This loss of CPK from ischemic myocardium of the cats given vehicle was reflected in plasma CPK specific activities which were significantly greater than those of sham-operated cats. The cats with ischemia and treated with PGI2 exhibited lower plasma CPK activities. These changes were moderated by PGI2 infusion during myocardial ischemia. PGI2 infusion may protect the ischemic myocardium by reducing oxygen demand, primarily through reductions in cardiac work, and by perhaps inhibiting platelet aggregation and preserving myocardial cell integrity.", "contents": "Studies on the protective effect of prostacyclin in acute myocardial ischemia. The ability of prostacyclin (PGI2) to alter responses to acute myocardial ischemia was studied in open-chest, anesthetized cats. PGI2 was infused intravenously at 0.5 nmoles kg-1 min-1 in cats subjected to 5 h of myocardial ischemia by occlusion of the LAD coronary artery, and in sham-operated controls. GI2 infusion resulted in significantly decreased arterial blood pressure and inhibition of platelet aggregation. Coronary ligation resulted in significant S-T segment elevations lasting 5 h in vehicle-treated animals but only 1 h in cats with myocardial ischemia and receiving PGI2. At 5 h, cats with ischemia and given the vehicle showed S-T segment elevations significantly greater than the other two groups. Ischemic myocardium from vehicle-treated animals exhibited significantly less creatine phosphokinase (CPK) specific activity than normal tissue from the same hearts or myocardial tissue from the other two groups. This loss of CPK from ischemic myocardium of the cats given vehicle was reflected in plasma CPK specific activities which were significantly greater than those of sham-operated cats. The cats with ischemia and treated with PGI2 exhibited lower plasma CPK activities. These changes were moderated by PGI2 infusion during myocardial ischemia. PGI2 infusion may protect the ischemic myocardium by reducing oxygen demand, primarily through reductions in cardiac work, and by perhaps inhibiting platelet aggregation and preserving myocardial cell integrity."}
{"id": "PMID:467530", "title": "Reticulospinal connections with limb and axial motoneurons.", "content": "Responses of motoneurons supplying muscles of the forelimbs, hindlimbs, back, and neck to stimulation of the medial pontomedullary reticular formation were studied with intracellular recording in cerebellectomized cats under chloralose anesthesia. Stimulation of the midline or of a reticular region consisting of nucleus reticularis (n.r.) pontis caudalis and the dorsorostral part of n.r. gigantocellularis produced monosynaptic excitation of ipsilateral motoneurons supplying axial muscles and flexor and extensor muscles in both proximal and distal parts of the limbs. This widespread excitation appears to have been produced by rapidly conducting medial reticulospinal fibers. Stimulation of a second region consisting of n.r. ventralis and the ventrocaudal part of n.r. gigantocellularis produced monosynaptic excitation of ipsilateral neck and back motoneurons but only longer latency, apparently multisynaptic excitation of limb motoneurons. Collision tests indicated that this monosynaptic excitation did not involve fibers descending along the midline. It therefore appears to have been produced by lateral reticulospinal fibers. Reticular stimulation also produced short latency, monosynaptic inhibition of neck motoneurons, long latency, apparently polysynaptic inhibition of limb motoneurons and intermediate latency inhibition of back motoneurons. The latencies and properties of inhibitory responses of back motoneurons indicated that they were produced either disynaptically by fast fibers or monosynaptically by slower fibers. The data indicate that the medial pontomedullary reticular formation can be divided into a number of different zones each with a distinct pattern of connections with somatic motoneurons. These include the dorsorostrally located medial reticulospinal projection area, from which direct excitation of a wide variety of motoneurons can be evoked, the ventrocaudally located lateral reticulospinal projection area from which direct excitation of neck and back and direct inhibition of neck motoneurons can be evoked and the dorsal strip of n.r. gigantocellularis which has direct excitatory and inhibitory actions only on neck motoneurons.", "contents": "Reticulospinal connections with limb and axial motoneurons. Responses of motoneurons supplying muscles of the forelimbs, hindlimbs, back, and neck to stimulation of the medial pontomedullary reticular formation were studied with intracellular recording in cerebellectomized cats under chloralose anesthesia. Stimulation of the midline or of a reticular region consisting of nucleus reticularis (n.r.) pontis caudalis and the dorsorostral part of n.r. gigantocellularis produced monosynaptic excitation of ipsilateral motoneurons supplying axial muscles and flexor and extensor muscles in both proximal and distal parts of the limbs. This widespread excitation appears to have been produced by rapidly conducting medial reticulospinal fibers. Stimulation of a second region consisting of n.r. ventralis and the ventrocaudal part of n.r. gigantocellularis produced monosynaptic excitation of ipsilateral neck and back motoneurons but only longer latency, apparently multisynaptic excitation of limb motoneurons. Collision tests indicated that this monosynaptic excitation did not involve fibers descending along the midline. It therefore appears to have been produced by lateral reticulospinal fibers. Reticular stimulation also produced short latency, monosynaptic inhibition of neck motoneurons, long latency, apparently polysynaptic inhibition of limb motoneurons and intermediate latency inhibition of back motoneurons. The latencies and properties of inhibitory responses of back motoneurons indicated that they were produced either disynaptically by fast fibers or monosynaptically by slower fibers. The data indicate that the medial pontomedullary reticular formation can be divided into a number of different zones each with a distinct pattern of connections with somatic motoneurons. These include the dorsorostrally located medial reticulospinal projection area, from which direct excitation of a wide variety of motoneurons can be evoked, the ventrocaudally located lateral reticulospinal projection area from which direct excitation of neck and back and direct inhibition of neck motoneurons can be evoked and the dorsal strip of n.r. gigantocellularis which has direct excitatory and inhibitory actions only on neck motoneurons."}
{"id": "PMID:467531", "title": "Ultrastructural data, with special reference to bouton/glial relationships, from the hypoglossal nucleus after a second axotomy of the hypoglossal nerve.", "content": "The left hypoglossal nerve of adult male albino rats was prevented from regenerating to the tongue after a distal axotomy by implanting the proximal stump into normally innervated left sternomasoid muscle. Eighty-four days after implantation, the hypoglossal nerve was transected again and its regeneration to the tongue unimpeded. From 8 to 70 days after this second axotomy the left hypoglossal nuclei were processed for quantitative ultrastructural analysis. The first aim of this study was to compare regeneration success in the hypoglossal nucleus after second axotomy with that accompanying outgrowth of the hypoglossal nerve into denervated sternomastoid muscle. During quantitative analysis a second aim developed, of elucidating bouton/glial relationships. The second axotomy induced loss and return of subsurface cisterns, dispersal and reassembly of Nissl substance, increase and decrease of microglial numbers, slight further loss and partial return of boutons with clear spherical vesicles and symmetrical synapses, slight increase and decrease of boutons with clear flat vesicles and symmetrical synapses, regrowth of retracted dendrites and restoration of their synapses, and gradual diminution of numbers of electron-dense neurones and dendrites. Astrocytes remained hypertrophied throughout. When compared with events in the hypoglossal nucleus accompanying innervation of denervated sternomastoid muscle by the hypoglossal nerve, the results suggest (1) that regeneration of the hypoglossal nerve to its own tongue muscle instead of to a foreign muscle caused no acceleration of recovery in the hypoglossal nucleus, and (2) that the microglial response is dependent on nerve integrity and not on bouton behaviour.", "contents": "Ultrastructural data, with special reference to bouton/glial relationships, from the hypoglossal nucleus after a second axotomy of the hypoglossal nerve. The left hypoglossal nerve of adult male albino rats was prevented from regenerating to the tongue after a distal axotomy by implanting the proximal stump into normally innervated left sternomasoid muscle. Eighty-four days after implantation, the hypoglossal nerve was transected again and its regeneration to the tongue unimpeded. From 8 to 70 days after this second axotomy the left hypoglossal nuclei were processed for quantitative ultrastructural analysis. The first aim of this study was to compare regeneration success in the hypoglossal nucleus after second axotomy with that accompanying outgrowth of the hypoglossal nerve into denervated sternomastoid muscle. During quantitative analysis a second aim developed, of elucidating bouton/glial relationships. The second axotomy induced loss and return of subsurface cisterns, dispersal and reassembly of Nissl substance, increase and decrease of microglial numbers, slight further loss and partial return of boutons with clear spherical vesicles and symmetrical synapses, slight increase and decrease of boutons with clear flat vesicles and symmetrical synapses, regrowth of retracted dendrites and restoration of their synapses, and gradual diminution of numbers of electron-dense neurones and dendrites. Astrocytes remained hypertrophied throughout. When compared with events in the hypoglossal nucleus accompanying innervation of denervated sternomastoid muscle by the hypoglossal nerve, the results suggest (1) that regeneration of the hypoglossal nerve to its own tongue muscle instead of to a foreign muscle caused no acceleration of recovery in the hypoglossal nucleus, and (2) that the microglial response is dependent on nerve integrity and not on bouton behaviour."}
{"id": "PMID:467532", "title": "Hypercomplex cell types in area 18 of the cat.", "content": "Single unit recording has revealed the same orientation sensitive cell classes in cat area 18 as are to be found in area 17. These include particularly the various types of hypercomplex cell belonging to the S, C, and B cell families.", "contents": "Hypercomplex cell types in area 18 of the cat. Single unit recording has revealed the same orientation sensitive cell classes in cat area 18 as are to be found in area 17. These include particularly the various types of hypercomplex cell belonging to the S, C, and B cell families."}
{"id": "PMID:467533", "title": "Electrophysiological evidence for axonal sprouting of cerebellothalamic neurons in kittens after neonatal hemicerebellectomy.", "content": "Changes in cerebello-cerebral responses after hemicerebellectomy were investigated in 19 kittens by laminar field potential analysis in the cerebral cortex. In all of 11 kittens operated on before 11 days of age and kept for more than 16 days after surgery, marked cerebello-cerebral responses were evoked not only contralaterally as in intact animals but also ipsilaterally. In none of 16 intact kittens ranging in age from 2 to 14 days was there a detectable response in the cerebral cortex to stimulation of the ipsilateral cerebellar nucleus. Pathways responsible for the ipsilateral cerebello-cerebral responses were investigated by destruction of the thalamic VL nucleus and by unitary recordings from cerebellar nuclear neurons with antidromic activation on stimulation of the thalamus. From the latter investigation, a remarkable increase in the number of neurons with bilateral projections upon the thalamus was seen in the surgically treated kittens. Stimulation of the VL thalamic nucleus contralateral to the remaining hemicerebellum induced a marked response in the frontal cortex not only ipsilaterally as in intact animals but also contralaterally. The latter response was considered to be mediated by an axon reflex of the bilateral projection neurons. Destruction of that VL nucleus abolished the contralateral cerebello-cerebral response whereas the ipsilateral cerebello-cerebral response remained unchanged. It is concluded, that in kittens, hemicerebellectomized in the early postnatal period, nuclear neurons in the remaining cerebellum sprout axon collaterals growing into the thalamus ipsilateral to the spared hemicerebellum and that these sprouting axon collaterals make synpases on the thalamic neurons projecting upon the cerebral cortex.", "contents": "Electrophysiological evidence for axonal sprouting of cerebellothalamic neurons in kittens after neonatal hemicerebellectomy. Changes in cerebello-cerebral responses after hemicerebellectomy were investigated in 19 kittens by laminar field potential analysis in the cerebral cortex. In all of 11 kittens operated on before 11 days of age and kept for more than 16 days after surgery, marked cerebello-cerebral responses were evoked not only contralaterally as in intact animals but also ipsilaterally. In none of 16 intact kittens ranging in age from 2 to 14 days was there a detectable response in the cerebral cortex to stimulation of the ipsilateral cerebellar nucleus. Pathways responsible for the ipsilateral cerebello-cerebral responses were investigated by destruction of the thalamic VL nucleus and by unitary recordings from cerebellar nuclear neurons with antidromic activation on stimulation of the thalamus. From the latter investigation, a remarkable increase in the number of neurons with bilateral projections upon the thalamus was seen in the surgically treated kittens. Stimulation of the VL thalamic nucleus contralateral to the remaining hemicerebellum induced a marked response in the frontal cortex not only ipsilaterally as in intact animals but also contralaterally. The latter response was considered to be mediated by an axon reflex of the bilateral projection neurons. Destruction of that VL nucleus abolished the contralateral cerebello-cerebral response whereas the ipsilateral cerebello-cerebral response remained unchanged. It is concluded, that in kittens, hemicerebellectomized in the early postnatal period, nuclear neurons in the remaining cerebellum sprout axon collaterals growing into the thalamus ipsilateral to the spared hemicerebellum and that these sprouting axon collaterals make synpases on the thalamic neurons projecting upon the cerebral cortex."}
{"id": "PMID:467534", "title": "Pineal response types in the frog's brain under white light exposure.", "content": "Responses to white light stimulation can be recorded with electrophysiological methods at the level of (1) the pineal system (2) different diencephalic nuclei and (3) the mesencephalic tegmentum. The neurons are classified in six groups according to their discharge characteristics. As some responses present a higher complexity than the classical messages, an integration of the pineal informations during their course toward the brain is suggested in order to support phototactic behavior.", "contents": "Pineal response types in the frog's brain under white light exposure. Responses to white light stimulation can be recorded with electrophysiological methods at the level of (1) the pineal system (2) different diencephalic nuclei and (3) the mesencephalic tegmentum. The neurons are classified in six groups according to their discharge characteristics. As some responses present a higher complexity than the classical messages, an integration of the pineal informations during their course toward the brain is suggested in order to support phototactic behavior."}
{"id": "PMID:467535", "title": "Hemisphere contributions to the composition of the pattern-evoked potential waveform.", "content": "The transverse distribution of scalp-recorded potentials evoked by pattern reversal stimulation was studied in 50 healthy subjects. In most individuals the full-field responses were symmetrical over the occipital scalp, but important variations in distribution, symmetry and waveform were recorded in some cases. Asymmetrical responses were similar for each eye (i.e., they were \"uncrossed\" or homonymous asymmetries). Full-field peak latencies and amplitudes in the lateral channels were more variable than those at midline electrodes. Half-field responses were markedly asymmetric with well-lateralised components widespread over occipital-parietal scalp. In contrast to the full-field responses, component values measured near the midline were less consistent than those from lateral channels due to waveform distortions in this area (\"transitional zone\"). Upper field stimulation is particularly likely to produce such midline waveform distortions. Activity recorded from the scalp contralateral to the half-field stimulated shows more inter-individual and inter-hemispheric variation than that recorded from ipsilateral electrodes. Variants in the full-field waveform can be accounted for by relative differences in amplitude and distribution of the ipsilateral and contralateral components from each half field. The algebraic sum of these half-field components does not differ significantly from the components of the separately recorded full-field response. Furthermore, responses from the surviving half-field in patients after total hemispherectomy contain all the ipsilateral and contralateral half-field components seen in healthy subjects.", "contents": "Hemisphere contributions to the composition of the pattern-evoked potential waveform. The transverse distribution of scalp-recorded potentials evoked by pattern reversal stimulation was studied in 50 healthy subjects. In most individuals the full-field responses were symmetrical over the occipital scalp, but important variations in distribution, symmetry and waveform were recorded in some cases. Asymmetrical responses were similar for each eye (i.e., they were \"uncrossed\" or homonymous asymmetries). Full-field peak latencies and amplitudes in the lateral channels were more variable than those at midline electrodes. Half-field responses were markedly asymmetric with well-lateralised components widespread over occipital-parietal scalp. In contrast to the full-field responses, component values measured near the midline were less consistent than those from lateral channels due to waveform distortions in this area (\"transitional zone\"). Upper field stimulation is particularly likely to produce such midline waveform distortions. Activity recorded from the scalp contralateral to the half-field stimulated shows more inter-individual and inter-hemispheric variation than that recorded from ipsilateral electrodes. Variants in the full-field waveform can be accounted for by relative differences in amplitude and distribution of the ipsilateral and contralateral components from each half field. The algebraic sum of these half-field components does not differ significantly from the components of the separately recorded full-field response. Furthermore, responses from the surviving half-field in patients after total hemispherectomy contain all the ipsilateral and contralateral half-field components seen in healthy subjects."}
{"id": "PMID:467536", "title": "The role of the corpus callosum in the development of interocular eye alignment and the organization of the visual field in the cat.", "content": "In young cats, the posterior portion of the corpus callosum was sectioned 13--29 days after birth. The animal's eyes were photographed at weekly intervals for six months using the pupil-reflex method. From the corneal reflection evident in the photographs the degree of alignment for the optical axes of each cat was estimated (Sherman, 1972). The 17 experimental cats all showed a significant tendency toward permanent divergent strabismus, as compared to six normal cats. The limits of the visual field were determined for both groups of cats using a perimetry technique similar to that of Sprague and Meikle (1965) and Sherman (1973). With one eye open normal cats responsed from 90 degrees ipsilateral to 45 degrees past the vertical midline into the contralateral visual field. With either eye the experimental cats responsed from 90 degrees ipsilateral to approximately the vertical midline. The loss of visual responsiveness is within the contralateral region of the normally binocular zone. Three cats received the same operation at 9, 13, or 20 months old. Eye alignment and visual field perimetry were unaffected by the surgery. It is not known whether the observed abnormalities result from arrested development, or disruption of intrinsically determined ocular alignment.", "contents": "The role of the corpus callosum in the development of interocular eye alignment and the organization of the visual field in the cat. In young cats, the posterior portion of the corpus callosum was sectioned 13--29 days after birth. The animal's eyes were photographed at weekly intervals for six months using the pupil-reflex method. From the corneal reflection evident in the photographs the degree of alignment for the optical axes of each cat was estimated (Sherman, 1972). The 17 experimental cats all showed a significant tendency toward permanent divergent strabismus, as compared to six normal cats. The limits of the visual field were determined for both groups of cats using a perimetry technique similar to that of Sprague and Meikle (1965) and Sherman (1973). With one eye open normal cats responsed from 90 degrees ipsilateral to 45 degrees past the vertical midline into the contralateral visual field. With either eye the experimental cats responsed from 90 degrees ipsilateral to approximately the vertical midline. The loss of visual responsiveness is within the contralateral region of the normally binocular zone. Three cats received the same operation at 9, 13, or 20 months old. Eye alignment and visual field perimetry were unaffected by the surgery. It is not known whether the observed abnormalities result from arrested development, or disruption of intrinsically determined ocular alignment."}
{"id": "PMID:467537", "title": "The effect of raising the scala tympani potassium concentration on the tone-induced cochlear responses of the guinea pig.", "content": "Scala tympani (ST) in guinea pig was perfused with modified Ringer's solutions containing 5--50 mM potassium; tone-induced cochlear responses from the basal turn of ST were compared before, during and after perfusions. The compound nerve action potential (N1) and afterpotential (a/p) amplitudes were reduced, especially above 20 mM; the summating potential (SP) was variable, but its onset shape changed consistently with 13--20 mM levels. However, the cochlear microphonic amplitude (CM) remained substantially unchanged even at the 35 mM level. K+ concentration was monitored in ST with ion-sensitive pipettes. Stable levels were reached within 2 min, but N1 responses continued to fall beyond this time. Recovery to normal K+ levels took place spontaneously and the concentration curve which resulted showed a 2-slope characteristic. These experiments question whether elevated potassium concentration in scala tympani depolarizes the hair cells, and if it does, whether the hear cell resting potential is involved in the generation of the CM.", "contents": "The effect of raising the scala tympani potassium concentration on the tone-induced cochlear responses of the guinea pig. Scala tympani (ST) in guinea pig was perfused with modified Ringer's solutions containing 5--50 mM potassium; tone-induced cochlear responses from the basal turn of ST were compared before, during and after perfusions. The compound nerve action potential (N1) and afterpotential (a/p) amplitudes were reduced, especially above 20 mM; the summating potential (SP) was variable, but its onset shape changed consistently with 13--20 mM levels. However, the cochlear microphonic amplitude (CM) remained substantially unchanged even at the 35 mM level. K+ concentration was monitored in ST with ion-sensitive pipettes. Stable levels were reached within 2 min, but N1 responses continued to fall beyond this time. Recovery to normal K+ levels took place spontaneously and the concentration curve which resulted showed a 2-slope characteristic. These experiments question whether elevated potassium concentration in scala tympani depolarizes the hair cells, and if it does, whether the hear cell resting potential is involved in the generation of the CM."}
{"id": "PMID:467538", "title": "Exchange and efflux of tryptophan from superfused cerebral cortex slices.", "content": "The efflux and exchange of L-tryptophan (Trp) from rat cerebral cortex slices were studied in a superfusion system. The substrate specificity of Trp exchange was assessed by measuring the stimulation of [3H]Trp exit provoked by other extracellular amino acids. Large neutral amino acids were the most potent, but also glutamic acid, lysine and glycine had some effect. The stimulation caused by extracellular Trp and phenylalanine persisted also at 0 degrees C though severalfold attenuated. Only intracellular histidine provoked slight inhibition of [3H]Trp efflux and intracellular Trp, phenylalanine and lysine had a small stimulatory effect. The results suggest an involvement of carrier-mediated processes in the exchange and efflux of Trp. The substrate specificities of the exchange and efflux are not apparently identical.", "contents": "Exchange and efflux of tryptophan from superfused cerebral cortex slices. The efflux and exchange of L-tryptophan (Trp) from rat cerebral cortex slices were studied in a superfusion system. The substrate specificity of Trp exchange was assessed by measuring the stimulation of [3H]Trp exit provoked by other extracellular amino acids. Large neutral amino acids were the most potent, but also glutamic acid, lysine and glycine had some effect. The stimulation caused by extracellular Trp and phenylalanine persisted also at 0 degrees C though severalfold attenuated. Only intracellular histidine provoked slight inhibition of [3H]Trp efflux and intracellular Trp, phenylalanine and lysine had a small stimulatory effect. The results suggest an involvement of carrier-mediated processes in the exchange and efflux of Trp. The substrate specificities of the exchange and efflux are not apparently identical."}
{"id": "PMID:467568", "title": "Tubular explant culture of fetal Syrian golden hamster tracheae.", "content": "Two types of organ culture technique (stationary and rocking) were compared for their ability to maintain the epithelial structures in the tubular explants of fetal Syrian golden hamster tracheae. A simple new technique has been devised for the organ culture of small tubular explants of fetal tracheae. A large pore-sized membrane filter is introduced into the rocking culture, which improves unfavourable in vitro effects of the stationary technique.", "contents": "Tubular explant culture of fetal Syrian golden hamster tracheae. Two types of organ culture technique (stationary and rocking) were compared for their ability to maintain the epithelial structures in the tubular explants of fetal Syrian golden hamster tracheae. A simple new technique has been devised for the organ culture of small tubular explants of fetal tracheae. A large pore-sized membrane filter is introduced into the rocking culture, which improves unfavourable in vitro effects of the stationary technique."}
{"id": "PMID:467569", "title": "The occurrence of lipid droplets in the proximal and distal tubules of the rat kidney after folic acid treatment.", "content": "Folic acid in high doses gives rise to an accumulation of lipid droplets in the kidney in addition to other changes in the epithelial of both proximal and distal tubules. With the administration of methionine a decrease of lipid droplets and an improvement of the structures of most of the membranes and mitochrondria are observed. These findings have been discussed in regard to the theory of the \"chemically induced hyperplasia of the kidneys\" related to folic acid.", "contents": "The occurrence of lipid droplets in the proximal and distal tubules of the rat kidney after folic acid treatment. Folic acid in high doses gives rise to an accumulation of lipid droplets in the kidney in addition to other changes in the epithelial of both proximal and distal tubules. With the administration of methionine a decrease of lipid droplets and an improvement of the structures of most of the membranes and mitochrondria are observed. These findings have been discussed in regard to the theory of the \"chemically induced hyperplasia of the kidneys\" related to folic acid."}
{"id": "PMID:467570", "title": "The glutathione status of the rat liver.", "content": "For the determination of cellular total glutathione, a new method is presented based on a fluorometric procedure. The relation between reduced glutathione, mixed glutathione disulfides and disulfide glutathione will be designated the glutathione status.", "contents": "The glutathione status of the rat liver. For the determination of cellular total glutathione, a new method is presented based on a fluorometric procedure. The relation between reduced glutathione, mixed glutathione disulfides and disulfide glutathione will be designated the glutathione status."}
{"id": "PMID:467571", "title": "Effect of temperature on the activity of AMP-deaminase from rat heart.", "content": "In the presence of 1 mM ATP, the plots of Michaelis dependence of the reaction catalysed by AMP-deaminase from rat heart were hyperbolic at all temperatures between 10 and 40 degrees C. Calculation of the energy of activation for ATP-activated enzyme and of the enzyme-substrate complex formation is presented.", "contents": "Effect of temperature on the activity of AMP-deaminase from rat heart. In the presence of 1 mM ATP, the plots of Michaelis dependence of the reaction catalysed by AMP-deaminase from rat heart were hyperbolic at all temperatures between 10 and 40 degrees C. Calculation of the energy of activation for ATP-activated enzyme and of the enzyme-substrate complex formation is presented."}
{"id": "PMID:467572", "title": "Recovery of renal lactate dehydrogenase (LDH) isoenzyme pattern after obstruction relief in experimental hydronephrosis.", "content": "The release of ureteral occlusion leads to a progressive recovery in LDH isoenzyme pattern with gradual increase of anodic fractions and decrease of middle and cathodic ones. Our findings demonstrate that the recovery is accomplished on the 10-14th day, in agreement with morphological and metabolic observation.", "contents": "Recovery of renal lactate dehydrogenase (LDH) isoenzyme pattern after obstruction relief in experimental hydronephrosis. The release of ureteral occlusion leads to a progressive recovery in LDH isoenzyme pattern with gradual increase of anodic fractions and decrease of middle and cathodic ones. Our findings demonstrate that the recovery is accomplished on the 10-14th day, in agreement with morphological and metabolic observation."}
{"id": "PMID:467573", "title": "Development of oxidative metabolism in the non-innervated optic lobe of the chick.", "content": "Early removal of the optic cup of the chick embryo prevents innervation of the contralateral optic lobe. This reduces the rate of development od citrate synthetase. The posthatch increase of the level of this enzyme related to oxidative metabolism is not impaired by denervation of the chick optic lobe.", "contents": "Development of oxidative metabolism in the non-innervated optic lobe of the chick. Early removal of the optic cup of the chick embryo prevents innervation of the contralateral optic lobe. This reduces the rate of development od citrate synthetase. The posthatch increase of the level of this enzyme related to oxidative metabolism is not impaired by denervation of the chick optic lobe."}
{"id": "PMID:467574", "title": "Isolation of di-N-2-propylpentyl phthalate from human urine.", "content": "A higher percentage of di-N-2 propylpentyl phthalate isolated from the urine of the normals and a lower quantity in the patients suffering from Eales' disease accompanied with the occurrence in patients of another compound which is also most likely a phthalate ester has been correlated with the Eales' disease.", "contents": "Isolation of di-N-2-propylpentyl phthalate from human urine. A higher percentage of di-N-2 propylpentyl phthalate isolated from the urine of the normals and a lower quantity in the patients suffering from Eales' disease accompanied with the occurrence in patients of another compound which is also most likely a phthalate ester has been correlated with the Eales' disease."}
{"id": "PMID:467575", "title": "2/8 translocation in a Japanese Burkitt's lymphoma.", "content": "A new translocation between chromosomes 2 and 8, t(2p-; 8q+), was found in fresh lymphoma cells from a Japanese patient with Epstein-Barr virus-carrying Burkitt's lymphoma, and in a lymphoma cell line derived from this patient. There was no 14q+ translocation, as has been previously described in African and North American Burkitt's lymphomas.", "contents": "2/8 translocation in a Japanese Burkitt's lymphoma. A new translocation between chromosomes 2 and 8, t(2p-; 8q+), was found in fresh lymphoma cells from a Japanese patient with Epstein-Barr virus-carrying Burkitt's lymphoma, and in a lymphoma cell line derived from this patient. There was no 14q+ translocation, as has been previously described in African and North American Burkitt's lymphomas."}
{"id": "PMID:467576", "title": "Differential scanning calorimetry of chromaffin granule membranes.", "content": "Differential scanning calorimetry thermograms of native chromaffin granule membranes exhibit several peaks in the 15-35 degrees C region. Extraction of cholesterol increases the size of the melting peaks. Addition of Ca2+ ions does not seem to influence the lipid transitions.", "contents": "Differential scanning calorimetry of chromaffin granule membranes. Differential scanning calorimetry thermograms of native chromaffin granule membranes exhibit several peaks in the 15-35 degrees C region. Extraction of cholesterol increases the size of the melting peaks. Addition of Ca2+ ions does not seem to influence the lipid transitions."}
{"id": "PMID:467577", "title": "The invasion and growth of Babesia bovis in tick tissue culture.", "content": "Erythrocytic forms of Babesia bovis inoculated into cell cultures of the tick Boophilus microplus invaded the tick cells and showed multiplication for up to 48 h after inoculation.", "contents": "The invasion and growth of Babesia bovis in tick tissue culture. Erythrocytic forms of Babesia bovis inoculated into cell cultures of the tick Boophilus microplus invaded the tick cells and showed multiplication for up to 48 h after inoculation."}
{"id": "PMID:467578", "title": "Histochemical and cytochemical observations on the acetylcholinesterase (AChE) localization in the superior cervical ganglion (SCG) after preganglionic sympathectomy of the neonatal rat.", "content": "Results of histochemical and cytochemical reactions show that after the section of preganglionic nerve trunk performed in newborn animals, the appearance and the individual increase in AChE activity in non-innervated nerve cells follow the pattern of normally innervated ganglion cell.", "contents": "Histochemical and cytochemical observations on the acetylcholinesterase (AChE) localization in the superior cervical ganglion (SCG) after preganglionic sympathectomy of the neonatal rat. Results of histochemical and cytochemical reactions show that after the section of preganglionic nerve trunk performed in newborn animals, the appearance and the individual increase in AChE activity in non-innervated nerve cells follow the pattern of normally innervated ganglion cell."}
{"id": "PMID:467579", "title": "Hageman factor activation and tight junction disruption in mice challenged with attenuated endotoxin.", "content": "Endotoxin treated with chromium chloride is less toxic to mice than the parent molecule, but can disrupt intestinal permeability barriers and has an enhanced ability to activate Hageman factor.", "contents": "Hageman factor activation and tight junction disruption in mice challenged with attenuated endotoxin. Endotoxin treated with chromium chloride is less toxic to mice than the parent molecule, but can disrupt intestinal permeability barriers and has an enhanced ability to activate Hageman factor."}
{"id": "PMID:467580", "title": "Binding of cytophilic rabbit IgG to homologous hepatocytes.", "content": "Rabbit liver cells were able to bind cytophilic monomeric and polymeric homologous IgG via their Fc receptor binding sites (FcR). On the other hand, non-cytophilic rabbit IgG did not bind to hepatocytes, even after its aggregation. The present findings suggest that FcR on rabbit liver cells are specific for cytophilic monomeric IgG but do not significantly bind non-cytophilic, polymeric IgG.", "contents": "Binding of cytophilic rabbit IgG to homologous hepatocytes. Rabbit liver cells were able to bind cytophilic monomeric and polymeric homologous IgG via their Fc receptor binding sites (FcR). On the other hand, non-cytophilic rabbit IgG did not bind to hepatocytes, even after its aggregation. The present findings suggest that FcR on rabbit liver cells are specific for cytophilic monomeric IgG but do not significantly bind non-cytophilic, polymeric IgG."}
{"id": "PMID:467581", "title": "Effects of increased potassium in scala tympani on auditory nerve sensitivity.", "content": "Raising the K+ concentration in scala tympani of the guinea-pig cochlea generally caused a substantial increase in the spontaneous firing rate of single auditory nerve fibres. This effect was not accompanied by any observed reduction in the threshold sensitivity of these fibres. These findings cast doubt on current theories of cochlear transduction.", "contents": "Effects of increased potassium in scala tympani on auditory nerve sensitivity. Raising the K+ concentration in scala tympani of the guinea-pig cochlea generally caused a substantial increase in the spontaneous firing rate of single auditory nerve fibres. This effect was not accompanied by any observed reduction in the threshold sensitivity of these fibres. These findings cast doubt on current theories of cochlear transduction."}
{"id": "PMID:467582", "title": "Why do patients with respiratory muscle paralysis require artificial hyperventilation?", "content": "The primary reason for the need of hyperventilation in patients with respiratory paralysis is the insufficiency of the inhibitory Hering-Breuer reflex. The artificial distension of the lungs with normal tidal volume cannot inhibit the respiratory centre in contrast to the normal state.", "contents": "Why do patients with respiratory muscle paralysis require artificial hyperventilation? The primary reason for the need of hyperventilation in patients with respiratory paralysis is the insufficiency of the inhibitory Hering-Breuer reflex. The artificial distension of the lungs with normal tidal volume cannot inhibit the respiratory centre in contrast to the normal state."}
{"id": "PMID:467583", "title": "Hyperventilation and inhibitory synapses.", "content": "Injection of a subconvulsive dose of strychnine (which blocked the inhibitory synapses) increases respiratory muscle activity evoked by stimulation of a sciatic nerve as well as by inhalation of hypercapnic gas mixture. Thus the inhibitory synapses prevent an excessive hyperventilation.", "contents": "Hyperventilation and inhibitory synapses. Injection of a subconvulsive dose of strychnine (which blocked the inhibitory synapses) increases respiratory muscle activity evoked by stimulation of a sciatic nerve as well as by inhalation of hypercapnic gas mixture. Thus the inhibitory synapses prevent an excessive hyperventilation."}
{"id": "PMID:467584", "title": "Frog's tongue receptive areas: neural organization and gustatory function.", "content": "Lateral and medial branch of the frog's IXth nerve innervates rostral third and caudal two-thirds of the tongue surface, respectively. The amounts of gustatory signals in these branches differ in proportion to the area they supply.", "contents": "Frog's tongue receptive areas: neural organization and gustatory function. Lateral and medial branch of the frog's IXth nerve innervates rostral third and caudal two-thirds of the tongue surface, respectively. The amounts of gustatory signals in these branches differ in proportion to the area they supply."}
{"id": "PMID:467586", "title": "Daily variation in the eye's 5-HT stores.", "content": "Eyes from mice have been assayed for 5-HT content at various times during the day. 5-HT levels are highest midway in the light period and lowest during the dark period. In general this daily variation conforms with other published reports for variation of 5-HT stores in brain and pineal.", "contents": "Daily variation in the eye's 5-HT stores. Eyes from mice have been assayed for 5-HT content at various times during the day. 5-HT levels are highest midway in the light period and lowest during the dark period. In general this daily variation conforms with other published reports for variation of 5-HT stores in brain and pineal."}
{"id": "PMID:467587", "title": "Pentagastrin activation of adenylate cyclase in human gastric biopsy specimens.", "content": "Adenylate cyclase activity of human fundic mucosa is log-normally distributed and equally stimulated by pentagastrin and histamine. Cimetidine inhibits the histamine, but not the pentagastrin effect, which is even intensified by H2-receptor blockade. The results indicate that pentagastrin and histamine activate adenylate cyclase via distinct receptors.", "contents": "Pentagastrin activation of adenylate cyclase in human gastric biopsy specimens. Adenylate cyclase activity of human fundic mucosa is log-normally distributed and equally stimulated by pentagastrin and histamine. Cimetidine inhibits the histamine, but not the pentagastrin effect, which is even intensified by H2-receptor blockade. The results indicate that pentagastrin and histamine activate adenylate cyclase via distinct receptors."}
{"id": "PMID:467588", "title": "Brain stem projections to the cerebral cortex in the rat.", "content": "By means of the HRP method it was shown that the entire cerebral cortex, but in greater proportion the frontal and posterior temporo-parietal regions, receive fibres from the dorsal and medial raphe nuclei and from the locus coeruleus. In contrast, the pars compacta substantiae nigrae and the tegmental area send projections to the motor and cingular areas respectively.", "contents": "Brain stem projections to the cerebral cortex in the rat. By means of the HRP method it was shown that the entire cerebral cortex, but in greater proportion the frontal and posterior temporo-parietal regions, receive fibres from the dorsal and medial raphe nuclei and from the locus coeruleus. In contrast, the pars compacta substantiae nigrae and the tegmental area send projections to the motor and cingular areas respectively."}
{"id": "PMID:467589", "title": "The human urinary gastric inhibitor displays blood-group activity.", "content": "The human urinary glycoprotein with gastric antisecretory activity displays a marked blood-group activity corresponding to the blood-group of the subject examined. Several studies exclude the possibility that the activity is due to contaminants.", "contents": "The human urinary gastric inhibitor displays blood-group activity. The human urinary glycoprotein with gastric antisecretory activity displays a marked blood-group activity corresponding to the blood-group of the subject examined. Several studies exclude the possibility that the activity is due to contaminants."}
{"id": "PMID:467590", "title": "Maternal behavior in two rat lines selected for differences in the acquisition of two-way avoidance.", "content": "The maternal behavior of Roman high- and low-avoidance (RHA/Verh and RLA/Verh) rats was studied, using  a time-sampling method. It was concluded that: a)RLA/Verh mothers spent more time with their young, b) RHA/Verh mothers were more active, and c) the mothers of both lines mostly \"blanketed' their young during nursing, although the RHA/Verh mothers assumed the side-nursing position more often than their counterparts.", "contents": "Maternal behavior in two rat lines selected for differences in the acquisition of two-way avoidance. The maternal behavior of Roman high- and low-avoidance (RHA/Verh and RLA/Verh) rats was studied, using  a time-sampling method. It was concluded that: a)RLA/Verh mothers spent more time with their young, b) RHA/Verh mothers were more active, and c) the mothers of both lines mostly \"blanketed' their young during nursing, although the RHA/Verh mothers assumed the side-nursing position more often than their counterparts."}
{"id": "PMID:467592", "title": "Velocity gradient and contraction frequency of the pyeloureteral system.", "content": "An optical detection method, using video imaging, is used to quantitatively record the frequency and velocity profile of the renal pelvis of the rabbit. It is demonstrated that concentric waves originating at the periphery of the pelvis have an initial velocity of 3.2 cm/sec, accelerating toward the pelviureteral junction reaching a final velocity of 6.4 cm/sec.", "contents": "Velocity gradient and contraction frequency of the pyeloureteral system. An optical detection method, using video imaging, is used to quantitatively record the frequency and velocity profile of the renal pelvis of the rabbit. It is demonstrated that concentric waves originating at the periphery of the pelvis have an initial velocity of 3.2 cm/sec, accelerating toward the pelviureteral junction reaching a final velocity of 6.4 cm/sec."}
{"id": "PMID:467593", "title": "Blocking by picrotoxin of nigra-evoked inhibition of neurons of ventromedial nucleus of the thalamus.", "content": "The transmitter substance realeased by nigro-thalamic fibres is proposed to be gamma-aminobutyric acid, since picrotoxin blocked nigra-evoked monosynaptic inhibition of thalamic neurons.", "contents": "Blocking by picrotoxin of nigra-evoked inhibition of neurons of ventromedial nucleus of the thalamus. The transmitter substance realeased by nigro-thalamic fibres is proposed to be gamma-aminobutyric acid, since picrotoxin blocked nigra-evoked monosynaptic inhibition of thalamic neurons."}
{"id": "PMID:467594", "title": "Antibiotic sensitivity testing by flow microcalorimetry.", "content": "The proposed flow microcalorimetric method for the diagnosis of bacteriuria has been extended to include antibiotic sensitivity testing. Sensitive organisms rapidly (4-8 min) show thermal responses to the added antibiotics over the normal range of concentrations (1 x, 2x, MIC value).", "contents": "Antibiotic sensitivity testing by flow microcalorimetry. The proposed flow microcalorimetric method for the diagnosis of bacteriuria has been extended to include antibiotic sensitivity testing. Sensitive organisms rapidly (4-8 min) show thermal responses to the added antibiotics over the normal range of concentrations (1 x, 2x, MIC value)."}
{"id": "PMID:467595", "title": "The effect of piroxicam on platelet aggregation.", "content": "Piroxicam inhibited aggregation of human and dog platelets caused by collagen, but not by adenosine diphosphate (ADP). Release of platelet ADP was inhibited by piroxicam.", "contents": "The effect of piroxicam on platelet aggregation. Piroxicam inhibited aggregation of human and dog platelets caused by collagen, but not by adenosine diphosphate (ADP). Release of platelet ADP was inhibited by piroxicam."}
{"id": "PMID:467596", "title": "Percutaneous penetration of indomethacin.", "content": "14C-Indomethacin (ID) was applied to the skin of guinea-pigs as an ointment. After several applications, the concentration of ID in the skin and muscle under the applied site reached a constant level. The concentration of ID in the muscle as well as the skin may be enough to exert an anti-inflammatory effect.", "contents": "Percutaneous penetration of indomethacin. 14C-Indomethacin (ID) was applied to the skin of guinea-pigs as an ointment. After several applications, the concentration of ID in the skin and muscle under the applied site reached a constant level. The concentration of ID in the muscle as well as the skin may be enough to exert an anti-inflammatory effect."}
{"id": "PMID:467597", "title": "Antihypertensive activity of some novel pyridinylidene arylurea derivatives in spontaneously hypertensive rats.", "content": "3 novel pyridinylidene arylurea derivatives were found to lower arterial pressure in spontaneously hypertensive rats. Their relative oral potency ranged from 6 to 32 times that of guanethidine. The onset of antihypertensive action following their oral administration was less than 1 h and the duration of action ranged from 8 to over 24 h. The antihypertensive activity of the pyridinylidene arylureas was found to be assoicated with depletion of tissue catecholamines. Compound C depleted cardiac norepinephrine with little or no effect on total brain norepinephrine levels. It is suggested that compound C may have useful antihypertensive properties without CNS depressant activity.", "contents": "Antihypertensive activity of some novel pyridinylidene arylurea derivatives in spontaneously hypertensive rats. 3 novel pyridinylidene arylurea derivatives were found to lower arterial pressure in spontaneously hypertensive rats. Their relative oral potency ranged from 6 to 32 times that of guanethidine. The onset of antihypertensive action following their oral administration was less than 1 h and the duration of action ranged from 8 to over 24 h. The antihypertensive activity of the pyridinylidene arylureas was found to be assoicated with depletion of tissue catecholamines. Compound C depleted cardiac norepinephrine with little or no effect on total brain norepinephrine levels. It is suggested that compound C may have useful antihypertensive properties without CNS depressant activity."}
{"id": "PMID:467598", "title": "Inhibition of human leucocytes locomotion by anti-inflammatory drugs.", "content": "In this study we have demonstrated that acidic non-steroidal anti-inflammatory drugs in low concentrations inhibited human PMN locomotion in vitro. A speculative mechanism of action is proposed.", "contents": "Inhibition of human leucocytes locomotion by anti-inflammatory drugs. In this study we have demonstrated that acidic non-steroidal anti-inflammatory drugs in low concentrations inhibited human PMN locomotion in vitro. A speculative mechanism of action is proposed."}
{"id": "PMID:467600", "title": "Renal injury after muscle extract infusion in rats: absence of toxicity with myoglobin.", "content": "A crude muscle extract infused into rats produced oliguria, a precipitous drop in total hemolytic complement, and in circulating white cell and platelets counts. A mild vaso-depressor effects was noted. These changes were not produced by myoglobin or saline infusion. Muscle constituents other than myoglobin are responsible for the systemic and renal nephrotoxic effects observed.", "contents": "Renal injury after muscle extract infusion in rats: absence of toxicity with myoglobin. A crude muscle extract infused into rats produced oliguria, a precipitous drop in total hemolytic complement, and in circulating white cell and platelets counts. A mild vaso-depressor effects was noted. These changes were not produced by myoglobin or saline infusion. Muscle constituents other than myoglobin are responsible for the systemic and renal nephrotoxic effects observed."}
{"id": "PMID:467601", "title": "Choleretic and cholestatic effects of infused bile salts in the rat.", "content": "In rats, at low infusion rates taurocholate (TC), taurochenodeoxycholate (TCDC) and taurodeoxycholate (TCD) each produced an increase in bile flow of 20-50%. However, at high infusion rates (5-20 mumoles min-1kg-1) the cholestatic effects of the bile salts were revealed and the relative toxicity of the bile salts was seen to be TDC greater than TCDC greater than TC.", "contents": "Choleretic and cholestatic effects of infused bile salts in the rat. In rats, at low infusion rates taurocholate (TC), taurochenodeoxycholate (TCDC) and taurodeoxycholate (TCD) each produced an increase in bile flow of 20-50%. However, at high infusion rates (5-20 mumoles min-1kg-1) the cholestatic effects of the bile salts were revealed and the relative toxicity of the bile salts was seen to be TDC greater than TCDC greater than TC."}
{"id": "PMID:467602", "title": "Changes in molecular species of pepsinogens in the development of the chick.", "content": "The potential peptic activity of the chick forestomach increases rapidly at the time of hatching. Together with this, the electrophoretic pattern of the pepsinogens shifts to the adult type. These changes take place even under the complete starvation of a newly hatch chick.", "contents": "Changes in molecular species of pepsinogens in the development of the chick. The potential peptic activity of the chick forestomach increases rapidly at the time of hatching. Together with this, the electrophoretic pattern of the pepsinogens shifts to the adult type. These changes take place even under the complete starvation of a newly hatch chick."}
{"id": "PMID:467603", "title": "Density gradient centrifugation of cells separated from multicellular tumor spheroids.", "content": "Cells from a murine fibrosarcome (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Following selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells were separated from small spheriods (400 mum diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 mum in diameter).", "contents": "Density gradient centrifugation of cells separated from multicellular tumor spheroids. Cells from a murine fibrosarcome (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Following selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells were separated from small spheriods (400 mum diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 mum in diameter)."}
{"id": "PMID:467604", "title": "Release of plasminogen activator from normal and neoplastic endometrium.", "content": "In the medium of endometrial carcinoma cultures, anti-urokinase-reacting plasminogen activator was released in contrast to cultures of normal or hyperplastic endometrium.", "contents": "Release of plasminogen activator from normal and neoplastic endometrium. In the medium of endometrial carcinoma cultures, anti-urokinase-reacting plasminogen activator was released in contrast to cultures of normal or hyperplastic endometrium."}
{"id": "PMID:467605", "title": "Immunological profile of breast cancer patients in early or advanced disease.", "content": "Immune reactivity of patients with early or advanced breast cancer, compared with healthy controls, has been measured using in vivo and in vitro tests. The results of our study show that impairment of cellular responsiveness occurred in women with advanced disease.", "contents": "Immunological profile of breast cancer patients in early or advanced disease. Immune reactivity of patients with early or advanced breast cancer, compared with healthy controls, has been measured using in vivo and in vitro tests. The results of our study show that impairment of cellular responsiveness occurred in women with advanced disease."}
{"id": "PMID:467606", "title": "Lymphatic metastasis of tumour; persistent transport of cells.", "content": "A model of lymphatic metastasis established by injecting Walker rat carcinoma cells into the rat footpad was used to study the output of tumour cells from the footpad. The lymphatic efferent from the footpad was cannulated in a group of rats with advanced neoplasm; it was shown that the output of tumour cells was continuous over periods up to 90 min and ranged from 10(2)-10(5) cells/min.", "contents": "Lymphatic metastasis of tumour; persistent transport of cells. A model of lymphatic metastasis established by injecting Walker rat carcinoma cells into the rat footpad was used to study the output of tumour cells from the footpad. The lymphatic efferent from the footpad was cannulated in a group of rats with advanced neoplasm; it was shown that the output of tumour cells was continuous over periods up to 90 min and ranged from 10(2)-10(5) cells/min."}
{"id": "PMID:467607", "title": "Copper-induced heart malformations in hamsters.", "content": "The injection of copper citrate into pregnant golden hamsters induces a specific pattern of cardiovascular malformations in their embroys. The syndrome consists of double-outlet right ventricle, pulmonary hypoplasia and a ventricular spetal defect.", "contents": "Copper-induced heart malformations in hamsters. The injection of copper citrate into pregnant golden hamsters induces a specific pattern of cardiovascular malformations in their embroys. The syndrome consists of double-outlet right ventricle, pulmonary hypoplasia and a ventricular spetal defect."}
{"id": "PMID:467608", "title": "Studies on the activity of phorbol myrystate acetate on the human polymorphonuclear leukocytes.", "content": "Phorbol myrystate acetate (PMA) activates nitroblue tetrazolium reduction in human polymorphs. The activation is inhibited by dibutyryl cyclic AMP, theophylline and phenylbutazone, but is not influenced by hydrocortisone in vitro, nor is it inhibited by leukocytes from patients treated with prednisone. Peptide analogues of Tuftsin also had no effect on this stimulatory activity. We conclude that the action of PMA on the nitroblue tetrazolium reduction is mediated through cyclic nucleotides.", "contents": "Studies on the activity of phorbol myrystate acetate on the human polymorphonuclear leukocytes. Phorbol myrystate acetate (PMA) activates nitroblue tetrazolium reduction in human polymorphs. The activation is inhibited by dibutyryl cyclic AMP, theophylline and phenylbutazone, but is not influenced by hydrocortisone in vitro, nor is it inhibited by leukocytes from patients treated with prednisone. Peptide analogues of Tuftsin also had no effect on this stimulatory activity. We conclude that the action of PMA on the nitroblue tetrazolium reduction is mediated through cyclic nucleotides."}
{"id": "PMID:467609", "title": "Preparation of lymphocytes from small volumes of peripheral mouse blood.", "content": "A method was developed which allowed for the isolation of lymphocytes (95% of leukocytes in the final suspensions) from small volumes of peripheral mouse blood (38% recovery). The method proved of value for studies of murine lymphocytes which required biochemical analysis of samples from individual animals on a serial basis.", "contents": "Preparation of lymphocytes from small volumes of peripheral mouse blood. A method was developed which allowed for the isolation of lymphocytes (95% of leukocytes in the final suspensions) from small volumes of peripheral mouse blood (38% recovery). The method proved of value for studies of murine lymphocytes which required biochemical analysis of samples from individual animals on a serial basis."}
{"id": "PMID:467610", "title": "Decrease of cholesterol and free fatty acids in cortisone-resistant lymphoid cells incubated with allogeneic tumor cells.", "content": "A marked decrease of cholesterol and free fatty acids was found in the cortisone-resistant lymphoid cells from thymus or spleen of mice immunized with Ehrlich carcinoma cells when incubated with the tumor cells.", "contents": "Decrease of cholesterol and free fatty acids in cortisone-resistant lymphoid cells incubated with allogeneic tumor cells. A marked decrease of cholesterol and free fatty acids was found in the cortisone-resistant lymphoid cells from thymus or spleen of mice immunized with Ehrlich carcinoma cells when incubated with the tumor cells."}
{"id": "PMID:467611", "title": "Shape change reaction of platelets in protein-free medium: ultramorphology.", "content": "Transmission and scanning electron microscopy indicate that rabbit platelets incubated in protein-poor medium retain their reactivity to shape change-inducing agents such as 5-hydroxytryptamine, adenosine-5'-diphosphate and chlorpromazine. Such platelets may be used as models for drug-membrane interactions, e.g. in neuronal cells.", "contents": "Shape change reaction of platelets in protein-free medium: ultramorphology. Transmission and scanning electron microscopy indicate that rabbit platelets incubated in protein-poor medium retain their reactivity to shape change-inducing agents such as 5-hydroxytryptamine, adenosine-5'-diphosphate and chlorpromazine. Such platelets may be used as models for drug-membrane interactions, e.g. in neuronal cells."}
{"id": "PMID:467612", "title": "Thymectomy modifies androgenizing effects of a testis transplant during critical period for neuroprogramming.", "content": "Thymectomy simultaneous with transplantation of a syngeneic testis from a littermate to Fischer 344 rats ameliorated the androgenizing effects of the testis transplant on ovarian morphology at 90 days of age.", "contents": "Thymectomy modifies androgenizing effects of a testis transplant during critical period for neuroprogramming. Thymectomy simultaneous with transplantation of a syngeneic testis from a littermate to Fischer 344 rats ameliorated the androgenizing effects of the testis transplant on ovarian morphology at 90 days of age."}
{"id": "PMID:467613", "title": "A new stimulation technique of the crista ampullaris of the lateral canal in the adult cat: study of the action potential of the vestibular nerve.", "content": "The stimulation of the crista ampullaris of the lateral canal by a short ampullopetal liquid flux produces on the pre-ganglionic vestibular fibres the appearance of a bimodal action potential. The amplitude of this action potential increases with the intensity of the stimulation. This stimulation also provokes the birth of an evoked potential at the level  of the vestibular nuclei as well as an ocular jerk.", "contents": "A new stimulation technique of the crista ampullaris of the lateral canal in the adult cat: study of the action potential of the vestibular nerve. The stimulation of the crista ampullaris of the lateral canal by a short ampullopetal liquid flux produces on the pre-ganglionic vestibular fibres the appearance of a bimodal action potential. The amplitude of this action potential increases with the intensity of the stimulation. This stimulation also provokes the birth of an evoked potential at the level  of the vestibular nuclei as well as an ocular jerk."}
{"id": "PMID:467614", "title": "A method for the determination of glutamate oxaloacetate transaminase in sperm plasma from boars and bulls.", "content": "A method is described for the determination of glutamate oxaloacetate transaminase (GOT) activity in sperm plasma from boars and bulls as a test for fertilizing ability.", "contents": "A method for the determination of glutamate oxaloacetate transaminase in sperm plasma from boars and bulls. A method is described for the determination of glutamate oxaloacetate transaminase (GOT) activity in sperm plasma from boars and bulls as a test for fertilizing ability."}
{"id": "PMID:467627", "title": "[Chemical and pharmacological research on pyran derivatives. XIII. Derivatives of 2H-pyrano/3,2-c/quinoline].", "content": "Treatment of N-alkylanilines or diphenylamine with N,N-dialkylethoxycarbonylacetamides in the presence of phosphorus oxychloride afforded 6-alkyl(phenyl)-4-dialkylaminopyrano [3,2-c] quinoline-2,5-(6H)-diones, two molecules of amide being involved in the reaction. In some instances 6-alkyl(phenyl)-4-alkylaminopyrano [3,2-c] quinoline-2,5(6H)-diones were obtained through a partial dealkylation of the amino group. Pharmacological evaluation of some compounds showed no activity on the CNS.", "contents": "[Chemical and pharmacological research on pyran derivatives. XIII. Derivatives of 2H-pyrano/3,2-c/quinoline]. Treatment of N-alkylanilines or diphenylamine with N,N-dialkylethoxycarbonylacetamides in the presence of phosphorus oxychloride afforded 6-alkyl(phenyl)-4-dialkylaminopyrano [3,2-c] quinoline-2,5-(6H)-diones, two molecules of amide being involved in the reaction. In some instances 6-alkyl(phenyl)-4-alkylaminopyrano [3,2-c] quinoline-2,5(6H)-diones were obtained through a partial dealkylation of the amino group. Pharmacological evaluation of some compounds showed no activity on the CNS."}
{"id": "PMID:467628", "title": "[Synthesis of O-(beta-diethylaminoethyl)cinchoninaldoxime with local anesthetic activity].", "content": "The synthesis and the pharmacological evaluation of a new series of O-(beta-diethylaminoethyl)aldoxines derived from cinchonine-aldehydes substituted at position 2 by alkyl and alkoxyl groups are described.", "contents": "[Synthesis of O-(beta-diethylaminoethyl)cinchoninaldoxime with local anesthetic activity]. The synthesis and the pharmacological evaluation of a new series of O-(beta-diethylaminoethyl)aldoxines derived from cinchonine-aldehydes substituted at position 2 by alkyl and alkoxyl groups are described."}
{"id": "PMID:467629", "title": "Amide, 1-adamantylamide of leu-enkephalin and leu-[D Ala2]enkephalin.", "content": "The synthesis of Leu-enkephalinamide, Leu-enkephalin-1-adamantylamide, Leu-[D Ala2]enkephalinamide and Leu-[D Ala2]enkephalin-1-adamantylamide are described. Preliminary results of pharmacological tests in vitro are reported.", "contents": "Amide, 1-adamantylamide of leu-enkephalin and leu-[D Ala2]enkephalin. The synthesis of Leu-enkephalinamide, Leu-enkephalin-1-adamantylamide, Leu-[D Ala2]enkephalinamide and Leu-[D Ala2]enkephalin-1-adamantylamide are described. Preliminary results of pharmacological tests in vitro are reported."}
{"id": "PMID:467630", "title": "Psychopharmacological study with K 8409, a 1H-naphtho[2,1-b]pyran 3-dialkylamino substituted derivative.", "content": "A 1H-naphtho[2,1-b]pyran derivative (K 8409) has undergone pharmacological investigation for psychotropic activity. The results show potential antidepressant action quantitatively similar to that of imipramine and amitriptyline, but due rather to MAO inhibition than to imipramine-like activity; interestingly enough, anti-MAO activity was particularly selective for the serotonin-converting enzyme, both centrally and peripherally. The outcome of the other tests suggests that the incidence of untoward effects is likely to be limited.", "contents": "Psychopharmacological study with K 8409, a 1H-naphtho[2,1-b]pyran 3-dialkylamino substituted derivative. A 1H-naphtho[2,1-b]pyran derivative (K 8409) has undergone pharmacological investigation for psychotropic activity. The results show potential antidepressant action quantitatively similar to that of imipramine and amitriptyline, but due rather to MAO inhibition than to imipramine-like activity; interestingly enough, anti-MAO activity was particularly selective for the serotonin-converting enzyme, both centrally and peripherally. The outcome of the other tests suggests that the incidence of untoward effects is likely to be limited."}
{"id": "PMID:467631", "title": "On the interpretability of quantitative structure-activity relationships (QSAR).", "content": "QSAR are a powerful tool to obtain information on the molecular mechanism of drug action as well as on properties of receptors. They can be evaluated at different levels of sophistication using different parameters to describe physical chemical properties of the drugs and different mathematical methods. In this presentation only QSAR at the level of extrathermodynamic parameters will be considered. Interpretation of QSAR is always connected with two basic questions: 1) Which requirements must be met in order to get meaningful (interpretable) QSAR and what can be done to satisfy these requirements? 2) What information can be gained (and in which way) from QSAR? Some of the most important aspects of these questions to be discussed in this paper are: 1) Interrelationships between parameters and the separability of hydrophobic, electronic and steric effects. 2) Description of hydrophobic interactions. 3) Separation of hydrophobic effects connected with transport and hydrophobic bonding. 4) Estimation of the biological point of attack. 5) Selection and informational content of biological response data. Factor analysis will be shown to be a useful data preprocessing step allowing one to systematize data and to recognize and to eliminate collinearities. Another multivariate method, principal component analysis, may be used to separate pharmacokinetic and pharmacodynamic effects.", "contents": "On the interpretability of quantitative structure-activity relationships (QSAR). QSAR are a powerful tool to obtain information on the molecular mechanism of drug action as well as on properties of receptors. They can be evaluated at different levels of sophistication using different parameters to describe physical chemical properties of the drugs and different mathematical methods. In this presentation only QSAR at the level of extrathermodynamic parameters will be considered. Interpretation of QSAR is always connected with two basic questions: 1) Which requirements must be met in order to get meaningful (interpretable) QSAR and what can be done to satisfy these requirements? 2) What information can be gained (and in which way) from QSAR? Some of the most important aspects of these questions to be discussed in this paper are: 1) Interrelationships between parameters and the separability of hydrophobic, electronic and steric effects. 2) Description of hydrophobic interactions. 3) Separation of hydrophobic effects connected with transport and hydrophobic bonding. 4) Estimation of the biological point of attack. 5) Selection and informational content of biological response data. Factor analysis will be shown to be a useful data preprocessing step allowing one to systematize data and to recognize and to eliminate collinearities. Another multivariate method, principal component analysis, may be used to separate pharmacokinetic and pharmacodynamic effects."}
{"id": "PMID:467632", "title": "QSAR in a series of muscarinic agents.", "content": "The results of a pharmacological investigation on a series of 3-substituted benzyltrimethylammonium salts possessing muscarinic activity are reported. Correlative analysis shows that the pharmacodynamic activity is a function of the hydrophobic-lipophilic and steric parameters associated with the substituents. In particular, it is revealed that a lipophilic pocket of limited size is present in the muscarinic receptor at an optimal distance from the electron--rich site with which the cationic termination interacts.", "contents": "QSAR in a series of muscarinic agents. The results of a pharmacological investigation on a series of 3-substituted benzyltrimethylammonium salts possessing muscarinic activity are reported. Correlative analysis shows that the pharmacodynamic activity is a function of the hydrophobic-lipophilic and steric parameters associated with the substituents. In particular, it is revealed that a lipophilic pocket of limited size is present in the muscarinic receptor at an optimal distance from the electron--rich site with which the cationic termination interacts."}
{"id": "PMID:467633", "title": "[Quinolozidinylalkylamines with antihypertensive activity].", "content": "Since lupinylamine [(I); R = H] exhibits hypotensive activity, mainly due to ganglionic blocking properties, and it is known that a high degree of steric hindrance around the basic function of other ganglioplegic amines is of paramount importance for optimal activity, several guinolizidine derivatives were prepared. They differ in the length of the alkyl chain connected to the ring and in the position of the amino group along the chain. Some N-substituted derivatives of 2-quinolizidin-1'alpha-yl-ethylamine (II) together with O-lupinylhydroxylamine and 2-quinolizidin-1'beta-yl-ethylamine, respectively isosteric and epimeric to it, were also prepared. When administered orally to spontaneously hypertensive rats, the amines (II), (III) and (XI) produced high and long-lasting antihypertensive activity, while the remaining compounds so far tested were inactive or had only modest effect on blood pressure. Compared with alpha-methyl-DOPA, amine (II) appears to be approximately two to three times as potent. The antihypertensive activity of (II) appears to be linked to glanglionic blocking properties, since this amine proved 1,2 times as potent as mecamylamine in the inhibition of cat nictitating membrane response to stimulation of the preganglionic sympathetic nerve.", "contents": "[Quinolozidinylalkylamines with antihypertensive activity]. Since lupinylamine [(I); R = H] exhibits hypotensive activity, mainly due to ganglionic blocking properties, and it is known that a high degree of steric hindrance around the basic function of other ganglioplegic amines is of paramount importance for optimal activity, several guinolizidine derivatives were prepared. They differ in the length of the alkyl chain connected to the ring and in the position of the amino group along the chain. Some N-substituted derivatives of 2-quinolizidin-1'alpha-yl-ethylamine (II) together with O-lupinylhydroxylamine and 2-quinolizidin-1'beta-yl-ethylamine, respectively isosteric and epimeric to it, were also prepared. When administered orally to spontaneously hypertensive rats, the amines (II), (III) and (XI) produced high and long-lasting antihypertensive activity, while the remaining compounds so far tested were inactive or had only modest effect on blood pressure. Compared with alpha-methyl-DOPA, amine (II) appears to be approximately two to three times as potent. The antihypertensive activity of (II) appears to be linked to glanglionic blocking properties, since this amine proved 1,2 times as potent as mecamylamine in the inhibition of cat nictitating membrane response to stimulation of the preganglionic sympathetic nerve."}
{"id": "PMID:467634", "title": "Use of iminodiacetic acid for the synthesis of piperazinyl peptides related to leu-enkephalin. II.", "content": "Piperazinyl peptides related to Leu-enkephalin were obtained using as intermediates condensation products with iminodiacetic acid. The products are devoid of analgesic activity.", "contents": "Use of iminodiacetic acid for the synthesis of piperazinyl peptides related to leu-enkephalin. II. Piperazinyl peptides related to Leu-enkephalin were obtained using as intermediates condensation products with iminodiacetic acid. The products are devoid of analgesic activity."}
{"id": "PMID:467636", "title": "Relationships between growth hormone secretion and microangiophaty in subjects with prediabetes.", "content": "This study was aimed at evaluating the relationship between growth hormone secretion and the presence of functional microangiopathy in prediabetes. The results here in presented clearly indicate that an exaggerated and abnormal secretion of this hormone was a constant feature of those prediabetics with functional angiopathy.", "contents": "Relationships between growth hormone secretion and microangiophaty in subjects with prediabetes. This study was aimed at evaluating the relationship between growth hormone secretion and the presence of functional microangiopathy in prediabetes. The results here in presented clearly indicate that an exaggerated and abnormal secretion of this hormone was a constant feature of those prediabetics with functional angiopathy."}
{"id": "PMID:467637", "title": "Drug-protein interaction: plasma protein binding of furocoumarins.", "content": "The binding of six furocoumarins (angelicin, psoralen, 8-methoxypsoralen, 5-methoxypsoralen, 8-methylpsoralen, 4,5'8-trimethylpsoralen) to human serum and human serum albumin was studied by equilibrium dialysis using tritium labelled compounds. The results indicated that in serum all furocoumarins are bound mostly by albumin, the extent of binding being related to the structure of the furocoumarins; at any rate, high values of the bound drug, ranging from 84 to 97% were observed. The percentage of binding is strictly related to the water solubility of the compounds. A limited number of binding sites, n = 1-2.4, were detected in the albumin molecule, indicating a high specificity in the binding process. The association constants of the furocoumarins to albumin. Ka, ranged from 1.2 X 10(4) M-1 (8-methoxypsoralen) to 1.9 X 10(5) M-1 (4,5'8-trimethylpsoralen).", "contents": "Drug-protein interaction: plasma protein binding of furocoumarins. The binding of six furocoumarins (angelicin, psoralen, 8-methoxypsoralen, 5-methoxypsoralen, 8-methylpsoralen, 4,5'8-trimethylpsoralen) to human serum and human serum albumin was studied by equilibrium dialysis using tritium labelled compounds. The results indicated that in serum all furocoumarins are bound mostly by albumin, the extent of binding being related to the structure of the furocoumarins; at any rate, high values of the bound drug, ranging from 84 to 97% were observed. The percentage of binding is strictly related to the water solubility of the compounds. A limited number of binding sites, n = 1-2.4, were detected in the albumin molecule, indicating a high specificity in the binding process. The association constants of the furocoumarins to albumin. Ka, ranged from 1.2 X 10(4) M-1 (8-methoxypsoralen) to 1.9 X 10(5) M-1 (4,5'8-trimethylpsoralen)."}
{"id": "PMID:467695", "title": "Menstrual history of young women exposed in utero to diethylstilbestrol.", "content": "The menstrual histories of 218 patients exposed in utero to diethylstilbestrol (DES) were compared with those of 158 control subjects. No significant differences were revealed in the complaint of menstrual irregularities at either the initial or follow-up examination. Age at menarche was the same in both groups, and was the same as found in the United States as a whole. These findings differ from the report of a controlled trial in Chicago which suggests that there is a specific pattern of oligomenorrhea associated with DES exposure.", "contents": "Menstrual history of young women exposed in utero to diethylstilbestrol. The menstrual histories of 218 patients exposed in utero to diethylstilbestrol (DES) were compared with those of 158 control subjects. No significant differences were revealed in the complaint of menstrual irregularities at either the initial or follow-up examination. Age at menarche was the same in both groups, and was the same as found in the United States as a whole. These findings differ from the report of a controlled trial in Chicago which suggests that there is a specific pattern of oligomenorrhea associated with DES exposure."}
{"id": "PMID:467696", "title": "The role of counseling in infertility.", "content": "Sixty-two couples with infertility received infertility counseling along with the infertility investigation and treatment. At the end of the study a questionnaire was sent to all patients in order to evaluate the incidence of psychologic symptoms associated with their infertility and their response to counseling. The study confirmed the presence of a high incidence of emotional symptomatology in the self-selected patients. It showed that male partners are also affected, but less so. Finally, it has demonstrated that infertility counseling serves to enhance the quality of life in many patients who have become the victims of their \"infertility crises.\"", "contents": "The role of counseling in infertility. Sixty-two couples with infertility received infertility counseling along with the infertility investigation and treatment. At the end of the study a questionnaire was sent to all patients in order to evaluate the incidence of psychologic symptoms associated with their infertility and their response to counseling. The study confirmed the presence of a high incidence of emotional symptomatology in the self-selected patients. It showed that male partners are also affected, but less so. Finally, it has demonstrated that infertility counseling serves to enhance the quality of life in many patients who have become the victims of their \"infertility crises.\""}
{"id": "PMID:467697", "title": "Therapeutic donor insemination: results and causes of nonfertilization.", "content": "The results of therapeutic donor insemination (AID) in 108 women are presented. Of the 108 women, 65 (60%) achieved a pregnancy. Eighty-five per cent of the pregnancies occurred with six AIDs or less, and 80% resulted in live births. The reasons why 43 women did not conceive are analyzed. The most common cause of failure was discontinuing the AID program. Of the original 108 women, 34 (32%) had dropped out of the program by the time they had received 6 AIDs or less. Reproductive abnormalities in the wife, and irregular ovulatory patterns, were the next most common causes of failure, and were often associated with discontinuation of the program. Advice regarding 2 days of sexual abstinence prior to AID was considered to improve the pregnancy rate. Fresh semen was shown to be superior to frozen samples in achieving pregnancies.", "contents": "Therapeutic donor insemination: results and causes of nonfertilization. The results of therapeutic donor insemination (AID) in 108 women are presented. Of the 108 women, 65 (60%) achieved a pregnancy. Eighty-five per cent of the pregnancies occurred with six AIDs or less, and 80% resulted in live births. The reasons why 43 women did not conceive are analyzed. The most common cause of failure was discontinuing the AID program. Of the original 108 women, 34 (32%) had dropped out of the program by the time they had received 6 AIDs or less. Reproductive abnormalities in the wife, and irregular ovulatory patterns, were the next most common causes of failure, and were often associated with discontinuation of the program. Advice regarding 2 days of sexual abstinence prior to AID was considered to improve the pregnancy rate. Fresh semen was shown to be superior to frozen samples in achieving pregnancies."}
{"id": "PMID:467699", "title": "Induction of ovulation in women with hyperprolactinemic amenorrhea using clomiphene and human chorionic gonadotropin of bromocriptine.", "content": "Clomiphene citrate (Clomid), when given alone, is generally considered ineffective in inducing ovulation in women with hyperprolactinemia. This study reports the treatment of 29 infertile women with hyperprolactinemic amenorrhea. Twenty-one patients (eighteen of whom had previously had no ovulation response to Clomid alone) were treated with a combined regimen of Clomid (100 to 200 mg/day for 5 days) and two injections of 5000 IU of human chorionic gonadotropin (HCG), the first 8 to 10 days after Clomid withdrawal and a second injection 1 week later. Basal body temperature charts, conception, and/or plasma progesterone measurements showed that 19 patients ovulated (90%). There were 17 pregnancies in 12 of 21 patients (57% pregnancy rate) with 15 single live births and two abortions. When bromocriptine (Parlodel) became available, a total of 22 patients (including 14 patients previously treated with Clomid/HCG, six of them successfully) with amenorrhea associated with hyperprolactinemia were treated with this drug with dosages varying from 2.5 mg to 15 mg/day. Ovulation was confirmed in 20 patients (90%). There were 17 pregnancies in 15 patients (68% pregnancy rate) with 15 single live births and two first-trimester abortions. In all, 21 of 29 patients (73%) achieved one or more pregnancies resulting in live births with one or both of the above treatments. It is concluded that a combined Clomid/HCG regimen can often be used as an effective alternative to bromocriptine therapy in the treatment of infertility associated with hyperprolactinemic amenorrhea.", "contents": "Induction of ovulation in women with hyperprolactinemic amenorrhea using clomiphene and human chorionic gonadotropin of bromocriptine. Clomiphene citrate (Clomid), when given alone, is generally considered ineffective in inducing ovulation in women with hyperprolactinemia. This study reports the treatment of 29 infertile women with hyperprolactinemic amenorrhea. Twenty-one patients (eighteen of whom had previously had no ovulation response to Clomid alone) were treated with a combined regimen of Clomid (100 to 200 mg/day for 5 days) and two injections of 5000 IU of human chorionic gonadotropin (HCG), the first 8 to 10 days after Clomid withdrawal and a second injection 1 week later. Basal body temperature charts, conception, and/or plasma progesterone measurements showed that 19 patients ovulated (90%). There were 17 pregnancies in 12 of 21 patients (57% pregnancy rate) with 15 single live births and two abortions. When bromocriptine (Parlodel) became available, a total of 22 patients (including 14 patients previously treated with Clomid/HCG, six of them successfully) with amenorrhea associated with hyperprolactinemia were treated with this drug with dosages varying from 2.5 mg to 15 mg/day. Ovulation was confirmed in 20 patients (90%). There were 17 pregnancies in 15 patients (68% pregnancy rate) with 15 single live births and two first-trimester abortions. In all, 21 of 29 patients (73%) achieved one or more pregnancies resulting in live births with one or both of the above treatments. It is concluded that a combined Clomid/HCG regimen can often be used as an effective alternative to bromocriptine therapy in the treatment of infertility associated with hyperprolactinemic amenorrhea."}
{"id": "PMID:467700", "title": "Blood carbon dioxide tension changes during hysteroscopy.", "content": "During hysteroscopy the uterus may be distended with carbon dioxide (CO2), nitrous oxide (N2O), or Hyskon (a high molecular weight dextran). An initial study in 27 patients (group 1) using arterialized venous blood samples demonstrated rises in carbon dioxide tension (PCO2) when N2O was insufflated by using a laparoscopy insufflating device--a constant-pressure, variable-volume gas source. Cardiovascular collapse occurred in one patient in this group, most probably as a result of macropulmonary emboli of N2O. The rise in PCO2 is accounted for by an increase in physiologic dead space. In another 24 patients (group 2) the gaseous media were introduced by using a constant-volume, variable-pressure gas source; this resulted in minimal changes in arterial PCO2. The choice of whether a gaseous or liquid distending medium is used for hysteroscopy is governed by the state of the endometrium. If a gaseous medium is indicated, then CO2 is preferable to N2O and should be introduced with a constant-volume, variable-pressure gas source.", "contents": "Blood carbon dioxide tension changes during hysteroscopy. During hysteroscopy the uterus may be distended with carbon dioxide (CO2), nitrous oxide (N2O), or Hyskon (a high molecular weight dextran). An initial study in 27 patients (group 1) using arterialized venous blood samples demonstrated rises in carbon dioxide tension (PCO2) when N2O was insufflated by using a laparoscopy insufflating device--a constant-pressure, variable-volume gas source. Cardiovascular collapse occurred in one patient in this group, most probably as a result of macropulmonary emboli of N2O. The rise in PCO2 is accounted for by an increase in physiologic dead space. In another 24 patients (group 2) the gaseous media were introduced by using a constant-volume, variable-pressure gas source; this resulted in minimal changes in arterial PCO2. The choice of whether a gaseous or liquid distending medium is used for hysteroscopy is governed by the state of the endometrium. If a gaseous medium is indicated, then CO2 is preferable to N2O and should be introduced with a constant-volume, variable-pressure gas source."}
{"id": "PMID:467701", "title": "End-to-end tubal anastomosis using an absorbable stent.", "content": "Using absorbable suture as a stent, end-to-end anastomosis of previously ligated or cauterized fallopian tubes was performed in 15 cases. Luminal patency of at least one fallopian tube has been achieved in all cases, and thus far seven pregnancies have occurred in six cases. The technique for inserting the absorbable suture into the distal and proximal portions of the tube is fully described, and the importance of repeated hydrotubations to maintain tubal patency is emphasized.", "contents": "End-to-end tubal anastomosis using an absorbable stent. Using absorbable suture as a stent, end-to-end anastomosis of previously ligated or cauterized fallopian tubes was performed in 15 cases. Luminal patency of at least one fallopian tube has been achieved in all cases, and thus far seven pregnancies have occurred in six cases. The technique for inserting the absorbable suture into the distal and proximal portions of the tube is fully described, and the importance of repeated hydrotubations to maintain tubal patency is emphasized."}
{"id": "PMID:467702", "title": "An estimate of the fertility potential of the fractions of the split ejaculate in terms of the motile sperm count.", "content": "The motile sperm count is a simple index which is equivalent to the product of sperm density (millions of sperm per milliliter) and percentage of motility (motile sperm per 100 sperm x 0.01). Since other studies have demonstrated a positive correlation between this index and the pregnancy rate, it has been hypothesized that the motile sperm count could be useful for expressing the potential fertility of individual semen specimens. Collection of semen by the split ejaculate technique and subsequent artificial insemination homologous (AIH) with the best split fraction has been used for the treatment of oligospermia. We calculated the motile sperm count for whole semen and the first fraction of split ejaculates from 45 men who were studied for infertility. Subsequently, 15 couples agreed to AIH with the first fraction of the split ejaculate. The data indicate that the motile sperm count may be a reasonable index for comparing the potential fertility of the semen specimens and thereby estimating the chances of successful AIH.", "contents": "An estimate of the fertility potential of the fractions of the split ejaculate in terms of the motile sperm count. The motile sperm count is a simple index which is equivalent to the product of sperm density (millions of sperm per milliliter) and percentage of motility (motile sperm per 100 sperm x 0.01). Since other studies have demonstrated a positive correlation between this index and the pregnancy rate, it has been hypothesized that the motile sperm count could be useful for expressing the potential fertility of individual semen specimens. Collection of semen by the split ejaculate technique and subsequent artificial insemination homologous (AIH) with the best split fraction has been used for the treatment of oligospermia. We calculated the motile sperm count for whole semen and the first fraction of split ejaculates from 45 men who were studied for infertility. Subsequently, 15 couples agreed to AIH with the first fraction of the split ejaculate. The data indicate that the motile sperm count may be a reasonable index for comparing the potential fertility of the semen specimens and thereby estimating the chances of successful AIH."}
{"id": "PMID:467703", "title": "Prolactin, fructose, and zinc levels found in human seminal plasma.", "content": "Fructose, zinc, and prolactin determinations were performed on the seminal plasma of normospermic (n = 30), oligospermic (n = 30), and azoospermic (n = 30) men to determine whether there was a correlation between any of these substances and seminal quality. None of these azoospermic men had congenital bilateral absence of the vasa, but in a separate group of such men (n = 10) these same studies were performed. Fructose was determined photometrically, zinc by atomic absorption, and prolactin by radioimmunoassay. Zinc and fructose levels in all three groups showed no statistical differences. Prolactin was found to be significantly higher in the seminal plasma of normospermic men than was found in the seminal plasma of oligospermic or azoospermic men. In men with congenital bilateral absence of the vasa and seminal vesicles, zinc was elevated, fructose was absent, and prolactin was markedly depressed.", "contents": "Prolactin, fructose, and zinc levels found in human seminal plasma. Fructose, zinc, and prolactin determinations were performed on the seminal plasma of normospermic (n = 30), oligospermic (n = 30), and azoospermic (n = 30) men to determine whether there was a correlation between any of these substances and seminal quality. None of these azoospermic men had congenital bilateral absence of the vasa, but in a separate group of such men (n = 10) these same studies were performed. Fructose was determined photometrically, zinc by atomic absorption, and prolactin by radioimmunoassay. Zinc and fructose levels in all three groups showed no statistical differences. Prolactin was found to be significantly higher in the seminal plasma of normospermic men than was found in the seminal plasma of oligospermic or azoospermic men. In men with congenital bilateral absence of the vasa and seminal vesicles, zinc was elevated, fructose was absent, and prolactin was markedly depressed."}
{"id": "PMID:467704", "title": "Effects of antisera on human sperm penetration of zona-free hamster ova.", "content": "Human sperm, after treatment with a 10% concentration of rabbit or rhesus monkey normal sera and antisera, were evaluated for fertilizing potential by incidence of zona-free hamster ova penetrated by the sperm as evidenced by the presence of swollen sperm heads and male pronuclei. Compared with the basic medium alone, treating sperm with normal sera tended to increase the percentage of ova penetrated whereas antisera against sperm, sperm extract, and testis caused significant decreases in ova penetrated. A Fab preparation of these antisera exhibited similar inhibitory effects. Antisera Fab treatment of the zona-free ova prior to exposure to sperm had no effect on penetration rate.", "contents": "Effects of antisera on human sperm penetration of zona-free hamster ova. Human sperm, after treatment with a 10% concentration of rabbit or rhesus monkey normal sera and antisera, were evaluated for fertilizing potential by incidence of zona-free hamster ova penetrated by the sperm as evidenced by the presence of swollen sperm heads and male pronuclei. Compared with the basic medium alone, treating sperm with normal sera tended to increase the percentage of ova penetrated whereas antisera against sperm, sperm extract, and testis caused significant decreases in ova penetrated. A Fab preparation of these antisera exhibited similar inhibitory effects. Antisera Fab treatment of the zona-free ova prior to exposure to sperm had no effect on penetration rate."}
{"id": "PMID:467722", "title": "[Characteristics of cat sensomotor cortex neuron responses to monaural and binaural stimulation].", "content": "In cats, the technique of localization parameter of dichotic sounds showed the binaural stimulation to be more effective than the monaural one. The interaural difference in intensity and time modified characteristics of neuronal responses in the sensorymotor cortex (SMC), the responses being most obvious in 67--88% of cases at the minor, approximating zero, temporal and amplitude interaural shifts. The SMC neurons altered their spontaneous activity in response to signals simulating movement of the sound source in horizontal plane. 74% of these neurons seemed to prefer the velocity up to 48 degrees/sec. These features of the SMC neuronal responses can be of major importance for formation and regulation of the cortical programs for willed motor acts of the animals in the course of acoustic orientation.", "contents": "[Characteristics of cat sensomotor cortex neuron responses to monaural and binaural stimulation]. In cats, the technique of localization parameter of dichotic sounds showed the binaural stimulation to be more effective than the monaural one. The interaural difference in intensity and time modified characteristics of neuronal responses in the sensorymotor cortex (SMC), the responses being most obvious in 67--88% of cases at the minor, approximating zero, temporal and amplitude interaural shifts. The SMC neurons altered their spontaneous activity in response to signals simulating movement of the sound source in horizontal plane. 74% of these neurons seemed to prefer the velocity up to 48 degrees/sec. These features of the SMC neuronal responses can be of major importance for formation and regulation of the cortical programs for willed motor acts of the animals in the course of acoustic orientation."}
{"id": "PMID:467723", "title": "[Common pathway principle in the organization of callosal connections].", "content": "The direct cortical responses and transcallosal potentials were recorded in 170 symmetrical points of the cat motor cortex during the electrical stimulation of one hemisphere. The area of the direct responses was larger than that of transcallosal responses. The latter was concentrated in the center of the motor area, while the direct responses were located in peripheral areas. Thresholds for the direct responses were lower than those for the transcallosal responses. Variation of the strength of the stimulation led to the changes of localization of the EPs in both hemispheres. Callosotomy abolished or decreased the transcallosal potentials and increased or decreased the direct cortical potentials. This suggests the principle of the common pathway for the callosal system activity.", "contents": "[Common pathway principle in the organization of callosal connections]. The direct cortical responses and transcallosal potentials were recorded in 170 symmetrical points of the cat motor cortex during the electrical stimulation of one hemisphere. The area of the direct responses was larger than that of transcallosal responses. The latter was concentrated in the center of the motor area, while the direct responses were located in peripheral areas. Thresholds for the direct responses were lower than those for the transcallosal responses. Variation of the strength of the stimulation led to the changes of localization of the EPs in both hemispheres. Callosotomy abolished or decreased the transcallosal potentials and increased or decreased the direct cortical potentials. This suggests the principle of the common pathway for the callosal system activity."}
{"id": "PMID:467726", "title": "[Characteristics of habituation in visceral systems].", "content": "In anesthetized and immobilized cats, habituation of evoked responses to repeated stimulation of the splanchnic nerve occurred in the cerebral cortex. Upon cessation of the stimulation, the habituation waned spontaneously (spontaneous recovery). The rate of spontaneous recovery depended on number of habituating stimuli (effect of \"below-zero\"). Repeated series of habituation training produced a more rapid habituation of EPs (potentiation of habituation). An extrastimulus to cutaneous or splanchnic nerve leads to recovery of the evoked response (dehabituation or sensitization). Repeated application of the dehabituating stimulus produced a progressively lesser sensitization (habituation of dehabituation).", "contents": "[Characteristics of habituation in visceral systems]. In anesthetized and immobilized cats, habituation of evoked responses to repeated stimulation of the splanchnic nerve occurred in the cerebral cortex. Upon cessation of the stimulation, the habituation waned spontaneously (spontaneous recovery). The rate of spontaneous recovery depended on number of habituating stimuli (effect of \"below-zero\"). Repeated series of habituation training produced a more rapid habituation of EPs (potentiation of habituation). An extrastimulus to cutaneous or splanchnic nerve leads to recovery of the evoked response (dehabituation or sensitization). Repeated application of the dehabituating stimulus produced a progressively lesser sensitization (habituation of dehabituation)."}
{"id": "PMID:467727", "title": "[Sympathoactivating and sympathoinhibitory afferent fibers of the vagus and splanchnic nerves].", "content": "Changes in frequency of tonic impulses in efferent sympathetic fibers of the left splanchnic nerve on stimulation (10 V, 10--20 Hz, 1 ms) of afferent fibers of the right splanchnic nerve and of both trunks of the vagus nerve at the cervical and abdominal levels have been studied in experiments on cats. It is established that there are sympatho-inhibitory and sympatho-activatory fibers whose quantitative correlation is different in various trunks. Sympatho-inhibitory influencies are most marked on stimulation of vagus nerve ventral branch and sympatho-activatory ones on stimulation of right splanchnic nerve. At the cervical level, in vagal left trunk sympatho-inhibitory fibers preponderate but in the right one another correlation occurs. In abdominal cavity the distribution of both types of fibers appears to have organ principle. Fibers innervating stomach have sympatho-inhibitory action but those innervating small intestine--sympatho-activatory. This suggests the hypothesis about the role of these fibers in digestion regulation. The central cholinolytic amysil abolishes sympatho-inhibitory effects but not sympatho-activatory ones.", "contents": "[Sympathoactivating and sympathoinhibitory afferent fibers of the vagus and splanchnic nerves]. Changes in frequency of tonic impulses in efferent sympathetic fibers of the left splanchnic nerve on stimulation (10 V, 10--20 Hz, 1 ms) of afferent fibers of the right splanchnic nerve and of both trunks of the vagus nerve at the cervical and abdominal levels have been studied in experiments on cats. It is established that there are sympatho-inhibitory and sympatho-activatory fibers whose quantitative correlation is different in various trunks. Sympatho-inhibitory influencies are most marked on stimulation of vagus nerve ventral branch and sympatho-activatory ones on stimulation of right splanchnic nerve. At the cervical level, in vagal left trunk sympatho-inhibitory fibers preponderate but in the right one another correlation occurs. In abdominal cavity the distribution of both types of fibers appears to have organ principle. Fibers innervating stomach have sympatho-inhibitory action but those innervating small intestine--sympatho-activatory. This suggests the hypothesis about the role of these fibers in digestion regulation. The central cholinolytic amysil abolishes sympatho-inhibitory effects but not sympatho-activatory ones."}
{"id": "PMID:467729", "title": "[Comparative characteristics of blood and lymph clotting and fibrinolytic activity].", "content": "In dogs, the lymph coagulation occurs much more slowly than that of the blood. This is due to low concentration of the coagulation factors (I, II, V, VII, VIII, X, XI+XII) and to an increased level of heparin in the lymph. The fibrinolytic activity of lymph is somewhat higher than that of the blood which may be due to the low concentration of antiplasmines. The lymphocytes (thymocytes) prove to be able to substitute the blood platelets during coagulation of blood and lymph.", "contents": "[Comparative characteristics of blood and lymph clotting and fibrinolytic activity]. In dogs, the lymph coagulation occurs much more slowly than that of the blood. This is due to low concentration of the coagulation factors (I, II, V, VII, VIII, X, XI+XII) and to an increased level of heparin in the lymph. The fibrinolytic activity of lymph is somewhat higher than that of the blood which may be due to the low concentration of antiplasmines. The lymphocytes (thymocytes) prove to be able to substitute the blood platelets during coagulation of blood and lymph."}
{"id": "PMID:467730", "title": "[Sequence of the spread of excitation in bird heart ventricles].", "content": "Intramural multipolar technique revealed primary sites of excitation in the subendocardium of central areas of border line of the apical and middle thirds of the bird heart septum. From the zone of primary depolarization the excitation wave spread on the subendocardium of ventricles. A number of sites of negativity were observed over the epicardium. The main mass of myocardium of free walls and the lower two thirds of the septum are activated by means of myocardium of free walls and the lower two thirds of the septum are activated by means of multifocal depolarization. The base third of the septum is the last to be activated. These features of the excitation of bird myocardium can be accounted for by the special character of distribution of the Purkinje fibers in the muscles of myocardium ventricles.", "contents": "[Sequence of the spread of excitation in bird heart ventricles]. Intramural multipolar technique revealed primary sites of excitation in the subendocardium of central areas of border line of the apical and middle thirds of the bird heart septum. From the zone of primary depolarization the excitation wave spread on the subendocardium of ventricles. A number of sites of negativity were observed over the epicardium. The main mass of myocardium of free walls and the lower two thirds of the septum are activated by means of myocardium of free walls and the lower two thirds of the septum are activated by means of multifocal depolarization. The base third of the septum is the last to be activated. These features of the excitation of bird myocardium can be accounted for by the special character of distribution of the Purkinje fibers in the muscles of myocardium ventricles."}
{"id": "PMID:467731", "title": "[Role of changes in the hemodynamics of the pulmonary circulation in pulmonary gas exchange].", "content": "High pressure in the lung artery was maintained with the aid of i. v. serotonin administration (0.5 mg/ml) in dogs during 10 min. The simultaneous increase of the intravascular pressure in minor circulation and of the lung volume circulation was followed by an increasing blood volume in lungs, diffusion capacity of lungs, oxygen tension in the arterial blood, and satiation of the latter with oxygen. The combined action of the above two hemodynamic factors aids to opening of latent vascular areas, to a more regular distribution of perfusion over the lung areas according to the ventilation level. This mechanism seems to be one of the first compensatory responses to disturbances in the lung gas exchange.", "contents": "[Role of changes in the hemodynamics of the pulmonary circulation in pulmonary gas exchange]. High pressure in the lung artery was maintained with the aid of i. v. serotonin administration (0.5 mg/ml) in dogs during 10 min. The simultaneous increase of the intravascular pressure in minor circulation and of the lung volume circulation was followed by an increasing blood volume in lungs, diffusion capacity of lungs, oxygen tension in the arterial blood, and satiation of the latter with oxygen. The combined action of the above two hemodynamic factors aids to opening of latent vascular areas, to a more regular distribution of perfusion over the lung areas according to the ventilation level. This mechanism seems to be one of the first compensatory responses to disturbances in the lung gas exchange."}
{"id": "PMID:467733", "title": "[Regulation of respiration in the presence of a combination of hypercapnia and an inspiratory resistance load].", "content": "The resistant load (an increased aerodynamic resistance to the gas flow) augmented both the intrathoracic pressure (ITP) and activity of the diaphragm neurons (ADN) in anesthetized cats. The increase in these parameters after alteration of CO2 tension in the alveolar gas (PACO2) by 1 mm Hg was also augmented whereas the increase of respiratory volumes (VT) was reduced. After bilateral vagotomy the inspiratory resistant load somewhat inhibited the ADN, still more so at increasing PACO2, whereas the ITP absolute values and its increase grew up. The analysis of ITP and ADN relationships suggests a significant part of intercostal and auxiliary muscles in the inspiratory increase occurring on resistant load. This becomes clearer after shunting the afferent system of lungs.", "contents": "[Regulation of respiration in the presence of a combination of hypercapnia and an inspiratory resistance load]. The resistant load (an increased aerodynamic resistance to the gas flow) augmented both the intrathoracic pressure (ITP) and activity of the diaphragm neurons (ADN) in anesthetized cats. The increase in these parameters after alteration of CO2 tension in the alveolar gas (PACO2) by 1 mm Hg was also augmented whereas the increase of respiratory volumes (VT) was reduced. After bilateral vagotomy the inspiratory resistant load somewhat inhibited the ADN, still more so at increasing PACO2, whereas the ITP absolute values and its increase grew up. The analysis of ITP and ADN relationships suggests a significant part of intercostal and auxiliary muscles in the inspiratory increase occurring on resistant load. This becomes clearer after shunting the afferent system of lungs."}
{"id": "PMID:467734", "title": "[Analysis of afferent impulsation and muscle potentials of the small intestine in response to sucrose].", "content": "Changes of potentials in the longitudinal and circular muscles of the small intestine and impulse frequency in mesenteric afferent fibers during intraluminal administration of sucrose solution were studied in cats before and after effects of adreno- and cholinolytic agents. Reserpine, dihydroergotamine, and N-cholinoblocking agents (hexonium or benzohexonium) increased the excitatory effect of sucrose whereas atropine abolished the effect. Atropine in combination with dihydroergotamine did not abolish the activating effect of sucrose. The latter seems to be unrelated to stimulation of either cholinergic or adrenergic intramural neurons. Involvement of substance P is suggested.", "contents": "[Analysis of afferent impulsation and muscle potentials of the small intestine in response to sucrose]. Changes of potentials in the longitudinal and circular muscles of the small intestine and impulse frequency in mesenteric afferent fibers during intraluminal administration of sucrose solution were studied in cats before and after effects of adreno- and cholinolytic agents. Reserpine, dihydroergotamine, and N-cholinoblocking agents (hexonium or benzohexonium) increased the excitatory effect of sucrose whereas atropine abolished the effect. Atropine in combination with dihydroergotamine did not abolish the activating effect of sucrose. The latter seems to be unrelated to stimulation of either cholinergic or adrenergic intramural neurons. Involvement of substance P is suggested."}
{"id": "PMID:467735", "title": "[Responses of individual receptor units of rat mammary gland parenchyma to tactile stimulation].", "content": "Two types of slowly adapting mechanosensitive units were revealed in the parenchyma of the rat mammary gland (SAI and SAII). At constant mechanical displacement of the parenchyma surface SAI exhibited irregular and SAII--regular firing. The diameter of the receptive fields of the SAI and SAII was 1.0--1.5 mm with the central point of this area being the most sensitive. Trapezoidal stimuli elicited both dynamic and static responses proportional to the velocity of the movement and its displacement amplitude; the threshold of the SAI and SAII increased as stimuli of increasing velocity were used.", "contents": "[Responses of individual receptor units of rat mammary gland parenchyma to tactile stimulation]. Two types of slowly adapting mechanosensitive units were revealed in the parenchyma of the rat mammary gland (SAI and SAII). At constant mechanical displacement of the parenchyma surface SAI exhibited irregular and SAII--regular firing. The diameter of the receptive fields of the SAI and SAII was 1.0--1.5 mm with the central point of this area being the most sensitive. Trapezoidal stimuli elicited both dynamic and static responses proportional to the velocity of the movement and its displacement amplitude; the threshold of the SAI and SAII increased as stimuli of increasing velocity were used."}
{"id": "PMID:467758", "title": "The S\u00e9zary syndrome.", "content": "Symptoms of the S\u00e9zary syndrome are described in a case history. S\u00e9zary's syndrome is a lymphoproliferative process of the skin, which eventually develops into an erythrodermia. Strange lymphocytes with a cerebriform chromatin structure, often with T-cell characteristics, circulate in the blood stream. In few instances they are also found in lymphnodes and bone marrow. This primarily benign disease can become malignant, possibly due to an overaggressive cytostatic therapy. Besides the hitherto unknown endogenous causes, exogenous factors cannot be excluded.", "contents": "The S\u00e9zary syndrome. Symptoms of the S\u00e9zary syndrome are described in a case history. S\u00e9zary's syndrome is a lymphoproliferative process of the skin, which eventually develops into an erythrodermia. Strange lymphocytes with a cerebriform chromatin structure, often with T-cell characteristics, circulate in the blood stream. In few instances they are also found in lymphnodes and bone marrow. This primarily benign disease can become malignant, possibly due to an overaggressive cytostatic therapy. Besides the hitherto unknown endogenous causes, exogenous factors cannot be excluded."}
{"id": "PMID:467759", "title": "Ichthyosis linearis circumflexa Com\u00e8l and Netherton's syndrome; an ultrastructural study.", "content": "An electron microscopic study of 3 cases of ichthyosis linearis circumflexa Com\u00e8l (ILC) reveals the presence of round bodies in the prickle cells: a characteristic feature of ILC. The horny and the granular layers are absent and are replaced by parakeratotic cells with condensed cytoplasm, which an amorphous material dissociates. The early formation of a vesiculopustule is detected. The importance of the dermal inflammation is considered and it is stated that ILC is not a purely epidermal disorder of keratinization.", "contents": "Ichthyosis linearis circumflexa Com\u00e8l and Netherton's syndrome; an ultrastructural study. An electron microscopic study of 3 cases of ichthyosis linearis circumflexa Com\u00e8l (ILC) reveals the presence of round bodies in the prickle cells: a characteristic feature of ILC. The horny and the granular layers are absent and are replaced by parakeratotic cells with condensed cytoplasm, which an amorphous material dissociates. The early formation of a vesiculopustule is detected. The importance of the dermal inflammation is considered and it is stated that ILC is not a purely epidermal disorder of keratinization."}
{"id": "PMID:467760", "title": "Intranuclear particles in keratoacanthoma: possible association with malignant degeneration.", "content": "Electron microscopic investigation of 12 patients with keratoacanthoma is reported. Intranuclear virus-like particles, as described by Zelickson and Lynch, were seen in 3 cases; 2 of these subsequently underwent malignant degeneration. These findings suggest that it is particularly important to exclude the possibility of malignant change in those tumours when intranuclear particles are found.", "contents": "Intranuclear particles in keratoacanthoma: possible association with malignant degeneration. Electron microscopic investigation of 12 patients with keratoacanthoma is reported. Intranuclear virus-like particles, as described by Zelickson and Lynch, were seen in 3 cases; 2 of these subsequently underwent malignant degeneration. These findings suggest that it is particularly important to exclude the possibility of malignant change in those tumours when intranuclear particles are found."}
{"id": "PMID:467761", "title": "Diffuse biphasic cutaneous amyloidosis.", "content": "2 patients with primary localized cutaneous amyloidosis were seen to have widespread macular and lichenoid types of lesions. Diffuse areas of pigmentation appeared to transform gradually over years into lichen amyloidosus as a result of chronic irritation of the skin from scratching. There was no evidence of systemic amyloidosis.", "contents": "Diffuse biphasic cutaneous amyloidosis. 2 patients with primary localized cutaneous amyloidosis were seen to have widespread macular and lichenoid types of lesions. Diffuse areas of pigmentation appeared to transform gradually over years into lichen amyloidosus as a result of chronic irritation of the skin from scratching. There was no evidence of systemic amyloidosis."}
{"id": "PMID:467762", "title": "Morphaea-like cutaneous changes in a patient with systemic scleroderma.", "content": "A case of systemic scleroderma with peculiar morphaea-like lesions is reported. The patient at first started with typical acrosclerosis, some parts of which later became softened and apparently resumed the appearance of normal skin, so that morphaea-like cutaneous plaques which remained hard, were soft. These changes were surrounded by erythema resembling the 'lilac ring' as noted in characteristic active lesions of morphaea.", "contents": "Morphaea-like cutaneous changes in a patient with systemic scleroderma. A case of systemic scleroderma with peculiar morphaea-like lesions is reported. The patient at first started with typical acrosclerosis, some parts of which later became softened and apparently resumed the appearance of normal skin, so that morphaea-like cutaneous plaques which remained hard, were soft. These changes were surrounded by erythema resembling the 'lilac ring' as noted in characteristic active lesions of morphaea."}
{"id": "PMID:467763", "title": "Low-dose chlorambucil in the treatment of generalized granuloma annulare.", "content": "5 of 6 adults with generalized granuloma annulare responded favorably to therapy with low-dose chlorambucil. 5 of the patients had lesions that were recurrent and long-standing and that resisted conventional modalities of therapy. Low-dose chlorambucil may be effective in a short-term therapy program but should be used only in patients with persistent, widespread, or unusual forms of generalized granuloma annulare. The program requires close patient and hematologic supervision.", "contents": "Low-dose chlorambucil in the treatment of generalized granuloma annulare. 5 of 6 adults with generalized granuloma annulare responded favorably to therapy with low-dose chlorambucil. 5 of the patients had lesions that were recurrent and long-standing and that resisted conventional modalities of therapy. Low-dose chlorambucil may be effective in a short-term therapy program but should be used only in patients with persistent, widespread, or unusual forms of generalized granuloma annulare. The program requires close patient and hematologic supervision."}
{"id": "PMID:467764", "title": "Skin petechiae and ecchymoses (vasculitis) due to anticoagulant therapy.", "content": "The appearance of petechiae and ecchymoses following anticoagulant and fibrinolytic therapy is an unusual and rare complication. The mechanism of the vasculitis is obscure but may be related to a chemical vasotoxic reaction. During anticoagulant and fibrinolytic therapy, 6 patients developed petechiae and ecchymoses which was accompanied by pain and fever. There were no disturbances of blood clotting factors. In spite of the varied action of the different anticoagulants and fibrinolytic drugs, the clinical course and the histological examination demonstrated a similar identical vasculitis.", "contents": "Skin petechiae and ecchymoses (vasculitis) due to anticoagulant therapy. The appearance of petechiae and ecchymoses following anticoagulant and fibrinolytic therapy is an unusual and rare complication. The mechanism of the vasculitis is obscure but may be related to a chemical vasotoxic reaction. During anticoagulant and fibrinolytic therapy, 6 patients developed petechiae and ecchymoses which was accompanied by pain and fever. There were no disturbances of blood clotting factors. In spite of the varied action of the different anticoagulants and fibrinolytic drugs, the clinical course and the histological examination demonstrated a similar identical vasculitis."}
{"id": "PMID:467765", "title": "PUVA treatment in mycosis fungoides.", "content": "2 patients with mycosis fungoides at the plaque stage were treated with oral methoxalen and UVA irradiation. 3 months later both developed the skin tumours with highly malignant histological picture. In 1 patient at the tumour stage the skin tumours were spreading during the PUVA treatment. 3 patients at the first or eczematoid stage cleared completely or partially by PUVA treatment.", "contents": "PUVA treatment in mycosis fungoides. 2 patients with mycosis fungoides at the plaque stage were treated with oral methoxalen and UVA irradiation. 3 months later both developed the skin tumours with highly malignant histological picture. In 1 patient at the tumour stage the skin tumours were spreading during the PUVA treatment. 3 patients at the first or eczematoid stage cleared completely or partially by PUVA treatment."}
{"id": "PMID:467766", "title": "Single cilia in the articular cartilage of the cat.", "content": "Single cilia (SC) in the articular cartilage of the cat are described with regard to structure, frequency and intracellular location. Comparison is made with their occurrence in the cartilage of other species or in other tissues; special consideration is given to their developmental stages. In the 1-year-old cat examined, about 20% of the cells showed SC with a predominantly intracellular course and some of them did not reach the extracellular space at all. Cross-sections of the proximal region of SC reveal the familiary 9+0 pattern; towards the distal end, the number of doublets and the diameter of the shaft are decreasing. It is concluded that most of the SC encountered are undergoing involution.", "contents": "Single cilia in the articular cartilage of the cat. Single cilia (SC) in the articular cartilage of the cat are described with regard to structure, frequency and intracellular location. Comparison is made with their occurrence in the cartilage of other species or in other tissues; special consideration is given to their developmental stages. In the 1-year-old cat examined, about 20% of the cells showed SC with a predominantly intracellular course and some of them did not reach the extracellular space at all. Cross-sections of the proximal region of SC reveal the familiary 9+0 pattern; towards the distal end, the number of doublets and the diameter of the shaft are decreasing. It is concluded that most of the SC encountered are undergoing involution."}
{"id": "PMID:467767", "title": "Nonrandom correlation between selective distribution of virogenic 5-bromodeoxyuridine and DNA-binding nonhistone proteins in rat DNA.", "content": "In order to more accurately determine a mechanism for the bromodeoxyuridine (BrdU)-mediated activation of endogenous type C virus from normal rat embryo cells, nonhistone nuclear protein-DNA interactions were analyzed in vitro. Native, as well as kinetically fractioned DNA samples previously labeled with either [3H]-thymidine or [3H]-BrdU were combined with DNA-binding nonhistones and characterized according to distribution of the isotopes, and extent and localization of protein-binding sites. As before, [3H]-BrdU was relatively more concentrated in repetitive DNA as compared to [3H]-thymidine. Using a membrane filter retention assay, nearly 60% of complete, 37% of repetitive, and 12% of nonrepeated DNA-protein reconstituted complexes were retained on the filters regardless of isotopic precursor. However, a proportionately greater amount of [3H]-BrdU than [3H]-thymidine was recovered following extensive digestion of renatured complexes with DNase I, even though comparable amounts of DNA were acid-insoluble. The disproportionate binding of nonhistones to repetitive DNA, especially BrdU-substituted regions, may be related to the highly specific, well-characterized modifications in eukaryotic transcription attributed to the analog.", "contents": "Nonrandom correlation between selective distribution of virogenic 5-bromodeoxyuridine and DNA-binding nonhistone proteins in rat DNA. In order to more accurately determine a mechanism for the bromodeoxyuridine (BrdU)-mediated activation of endogenous type C virus from normal rat embryo cells, nonhistone nuclear protein-DNA interactions were analyzed in vitro. Native, as well as kinetically fractioned DNA samples previously labeled with either [3H]-thymidine or [3H]-BrdU were combined with DNA-binding nonhistones and characterized according to distribution of the isotopes, and extent and localization of protein-binding sites. As before, [3H]-BrdU was relatively more concentrated in repetitive DNA as compared to [3H]-thymidine. Using a membrane filter retention assay, nearly 60% of complete, 37% of repetitive, and 12% of nonrepeated DNA-protein reconstituted complexes were retained on the filters regardless of isotopic precursor. However, a proportionately greater amount of [3H]-BrdU than [3H]-thymidine was recovered following extensive digestion of renatured complexes with DNase I, even though comparable amounts of DNA were acid-insoluble. The disproportionate binding of nonhistones to repetitive DNA, especially BrdU-substituted regions, may be related to the highly specific, well-characterized modifications in eukaryotic transcription attributed to the analog."}
{"id": "PMID:467768", "title": "Inhibition by cerulenin of RNA and lipid synthesis in Limnaea (mollusc) embryos.", "content": "Cerulenin, a new antibiotic known to inhibit lipid synthesis in yeast, fungi and bacterial systems, has been tested with Limnaea embryos. In the presence of 40--50 microgram/ml cerulenin, lipid synthesis is inhibited by about 65% while RNA synthesis is inhibited by about 35% as is evident from the incorporation of 14C-acetate and 14C-uracil, respectively. In embryos treated with cerulenin, washed and returned to normal medium to which 14C-uracil was added, RNA synthesis continued normally. Such pretreatment with actinomycin, however, inhibits lipid synthesis by about 50%. Cerulenin treatment of eggs at the early morula or uncleaved stage for 30 min leads to a marked abnormality at the late veliger stage, and the embryos die before hatching. These results have been discussed in comparison with the known effects of actinomycin. As, unlike actinomycin, cerulenin has no delayed action, the present experiment offers a system for exploring the delayed effects of early transcription.", "contents": "Inhibition by cerulenin of RNA and lipid synthesis in Limnaea (mollusc) embryos. Cerulenin, a new antibiotic known to inhibit lipid synthesis in yeast, fungi and bacterial systems, has been tested with Limnaea embryos. In the presence of 40--50 microgram/ml cerulenin, lipid synthesis is inhibited by about 65% while RNA synthesis is inhibited by about 35% as is evident from the incorporation of 14C-acetate and 14C-uracil, respectively. In embryos treated with cerulenin, washed and returned to normal medium to which 14C-uracil was added, RNA synthesis continued normally. Such pretreatment with actinomycin, however, inhibits lipid synthesis by about 50%. Cerulenin treatment of eggs at the early morula or uncleaved stage for 30 min leads to a marked abnormality at the late veliger stage, and the embryos die before hatching. These results have been discussed in comparison with the known effects of actinomycin. As, unlike actinomycin, cerulenin has no delayed action, the present experiment offers a system for exploring the delayed effects of early transcription."}
{"id": "PMID:467769", "title": "Inhibiting effect of vitamins C and B12 on the mitotic activity of ascites tumors.", "content": "The mitotic activity of the transplantable mouse tumors, Sarcoma 37, Krebs-2, and Ehrlich carcinomas, in the ascites form, were inhibited after treatment with a mixture of vitamins C and B12 with no apparent toxic side effects. These vitamins when administered alone, at the same dosage, did not seem to have any apparent effect on mitosis or the morphology of the cells studied. Microscopic examination of the stained ascites fluid taken from the mice treated with the vitamin mixture showed few tumor cells, and these in various stages of disintegration. Also, an increase in lymphocytes, monocytes and neutrophils were noticed; however, later in the experiment, no tumor cells could be found and monocytes and macrophages were abundant.", "contents": "Inhibiting effect of vitamins C and B12 on the mitotic activity of ascites tumors. The mitotic activity of the transplantable mouse tumors, Sarcoma 37, Krebs-2, and Ehrlich carcinomas, in the ascites form, were inhibited after treatment with a mixture of vitamins C and B12 with no apparent toxic side effects. These vitamins when administered alone, at the same dosage, did not seem to have any apparent effect on mitosis or the morphology of the cells studied. Microscopic examination of the stained ascites fluid taken from the mice treated with the vitamin mixture showed few tumor cells, and these in various stages of disintegration. Also, an increase in lymphocytes, monocytes and neutrophils were noticed; however, later in the experiment, no tumor cells could be found and monocytes and macrophages were abundant."}
{"id": "PMID:467770", "title": "On the role of calcium in adhesion of cells to solid substrates.", "content": "Experiments are described which show that while the presence of calcium in the medium is required for the cells to maintain their adhesion, it is not necessary for the initial attachment of 3T3 cells to solid substrates. Cells are detached by treatment with urea at 4 degrees C suggesting that adhesion may involve hydrogen bonding between the cell surface and the substratum. Although most of the cell-bound calcium is removed by trypsin, the detaching effect of trypsinisation can be inhibited at low temperature suggesting that ionic calcium bridges are probably not directly involved in retaining the cells on the surface. Cells are made totally insensitive to removal by trypsin by prior washing with lanthanum. Our findings suggest that the external role of calcium in cell adhesion is exerted indirectly. We conclude that the cell presents to the exterior at least two physiochemical classes of molecule. One class composed of hydrogen bond-forming adhesive material (possible proteins) and another class of anti-adhesive molecules (possibly glycoproteins). These two components are somehow separated in the formation of adhesive 'plaques' and this process is process is apparently uninfluenced by the calcium concentration in the medium. However, the maintenance of the localised zones of adhesion is aided by factors which prevent their disruption by the intrusion into them of anti-adhesive molecules diffusing from adjacent regions of the cell membrane. These factors include cooling below the transition temperature of the membrane lipids and lateral cross-linking of non-adhesive elements by calcium. By contrast, conditions which reduce the stability of the separation of adhesive and non-adhesive surface components would be expected to diminish the overall adhesiveness of cells to the substratum.", "contents": "On the role of calcium in adhesion of cells to solid substrates. Experiments are described which show that while the presence of calcium in the medium is required for the cells to maintain their adhesion, it is not necessary for the initial attachment of 3T3 cells to solid substrates. Cells are detached by treatment with urea at 4 degrees C suggesting that adhesion may involve hydrogen bonding between the cell surface and the substratum. Although most of the cell-bound calcium is removed by trypsin, the detaching effect of trypsinisation can be inhibited at low temperature suggesting that ionic calcium bridges are probably not directly involved in retaining the cells on the surface. Cells are made totally insensitive to removal by trypsin by prior washing with lanthanum. Our findings suggest that the external role of calcium in cell adhesion is exerted indirectly. We conclude that the cell presents to the exterior at least two physiochemical classes of molecule. One class composed of hydrogen bond-forming adhesive material (possible proteins) and another class of anti-adhesive molecules (possibly glycoproteins). These two components are somehow separated in the formation of adhesive 'plaques' and this process is process is apparently uninfluenced by the calcium concentration in the medium. However, the maintenance of the localised zones of adhesion is aided by factors which prevent their disruption by the intrusion into them of anti-adhesive molecules diffusing from adjacent regions of the cell membrane. These factors include cooling below the transition temperature of the membrane lipids and lateral cross-linking of non-adhesive elements by calcium. By contrast, conditions which reduce the stability of the separation of adhesive and non-adhesive surface components would be expected to diminish the overall adhesiveness of cells to the substratum."}
{"id": "PMID:467771", "title": "Effects of cadmium on polyribosome sedimentation pattern in mouse liver.", "content": "Cadmium injected to mice provokes an acute disaggregation of hepatic polyribosomes. The effect appears within 15 min, it is maximal after 1 h and then gradually disappears. The reaggregation of polyribosomes takes place 6--12 h after injection. The disaggregation of polyribosomes is linear with log doses in a range of 7.25--20 mumol/kg CdCl2. Cycloheximide pretreatment prevents the disaggregation of polyribosomes in the livers of cadmium-treated mice.", "contents": "Effects of cadmium on polyribosome sedimentation pattern in mouse liver. Cadmium injected to mice provokes an acute disaggregation of hepatic polyribosomes. The effect appears within 15 min, it is maximal after 1 h and then gradually disappears. The reaggregation of polyribosomes takes place 6--12 h after injection. The disaggregation of polyribosomes is linear with log doses in a range of 7.25--20 mumol/kg CdCl2. Cycloheximide pretreatment prevents the disaggregation of polyribosomes in the livers of cadmium-treated mice."}
{"id": "PMID:467772", "title": "In vitro expression of cytotoxicity by macrophages is independent of the actual serum concentration.", "content": "In utilizing the same effector and target cells, similar arrangement and population density of cells but varying the actual serum concentration in the range between 1 and 50% fetal calf serum, it was attempted to accurately assess the dependence of in vitro cytolysis expressed by adherent, predominantly phagocytic mononuclear cells ('macrophages') on the actual serum concentration. The results show that the extent to which cytotoxicity by activated macrophages against a given target cell type was expressed, was within a comparable range independent of the actual serum concentration. This holds true for each of the 9 target cell lines examined although with some of the cell lines there was a certain tendency towards high cytotoxicity in the range between 5 and 20% serum.", "contents": "In vitro expression of cytotoxicity by macrophages is independent of the actual serum concentration. In utilizing the same effector and target cells, similar arrangement and population density of cells but varying the actual serum concentration in the range between 1 and 50% fetal calf serum, it was attempted to accurately assess the dependence of in vitro cytolysis expressed by adherent, predominantly phagocytic mononuclear cells ('macrophages') on the actual serum concentration. The results show that the extent to which cytotoxicity by activated macrophages against a given target cell type was expressed, was within a comparable range independent of the actual serum concentration. This holds true for each of the 9 target cell lines examined although with some of the cell lines there was a certain tendency towards high cytotoxicity in the range between 5 and 20% serum."}
{"id": "PMID:467773", "title": "Growth of human tumor xenografts implanted under the renal capsule of normal immunocompetent mice.", "content": "The subrenal capsule technique proved effective in demonstrating that the growth of human tumors in normal, immunocompetent animals for 6 days was quantifiable in ocular micrometer units. Positive growth was demonstrable not only with human tumors that had been established in serial transplantation in athymic nude mouse hosts, but also with primary surgical explants. Growth rates of transplantation-established xenograft systems were similar whether implanted in athymic nude or in normal immunocompetent animals indicating that the 6-day time-frame successfully evades growth inhibitory effects of immunologic origin. Immunosuppression with a single dose of cyclophosphamide did not appear to affect growth rate, but permitted the tumors to grow larger extending the time to reach peak size. Significantly, xenografts of primary surgical explants showed positive growth more frequently in 6 days (82%) in the immunocompetent animal than in 11 days (30%) in the immunodeficient athymic nude mouse.", "contents": "Growth of human tumor xenografts implanted under the renal capsule of normal immunocompetent mice. The subrenal capsule technique proved effective in demonstrating that the growth of human tumors in normal, immunocompetent animals for 6 days was quantifiable in ocular micrometer units. Positive growth was demonstrable not only with human tumors that had been established in serial transplantation in athymic nude mouse hosts, but also with primary surgical explants. Growth rates of transplantation-established xenograft systems were similar whether implanted in athymic nude or in normal immunocompetent animals indicating that the 6-day time-frame successfully evades growth inhibitory effects of immunologic origin. Immunosuppression with a single dose of cyclophosphamide did not appear to affect growth rate, but permitted the tumors to grow larger extending the time to reach peak size. Significantly, xenografts of primary surgical explants showed positive growth more frequently in 6 days (82%) in the immunocompetent animal than in 11 days (30%) in the immunodeficient athymic nude mouse."}
{"id": "PMID:467774", "title": "Phagocytosis and resorption during the reassembly of dissociated embryonic cells of sea urchins.", "content": "Time-lapse and electron microscopic observations were made on both epithelial and mesenchymal cells during the reassembly of embryos from dissociated cells of Strongylocentrotus purpuratus. In epithelial cells, where lysosomes are produced through the fusion of saccules formed from Golgi bodies, both phagocytosis of cell debris and resorption of differentiated cell structures were observed. In these cells, the lysosomes migrate and fuse with both autosomes and phagosomes. On the other hand, in the mesenchyme cells, where lysosomes are produced through the direct enlargement of the Golgi body's cisterna, neither phagocytosis nor resorption was observed. The migration of the lysosomes to the epithelial cell margins is the first indication of a re-establishment of cellular polarity after dissociation.", "contents": "Phagocytosis and resorption during the reassembly of dissociated embryonic cells of sea urchins. Time-lapse and electron microscopic observations were made on both epithelial and mesenchymal cells during the reassembly of embryos from dissociated cells of Strongylocentrotus purpuratus. In epithelial cells, where lysosomes are produced through the fusion of saccules formed from Golgi bodies, both phagocytosis of cell debris and resorption of differentiated cell structures were observed. In these cells, the lysosomes migrate and fuse with both autosomes and phagosomes. On the other hand, in the mesenchyme cells, where lysosomes are produced through the direct enlargement of the Golgi body's cisterna, neither phagocytosis nor resorption was observed. The migration of the lysosomes to the epithelial cell margins is the first indication of a re-establishment of cellular polarity after dissociation."}
{"id": "PMID:467779", "title": "Insulin binding by a cell line (HT 29) derived from human colonic cancer.", "content": "Insulin binding was demonstrated in cultured HT 29 cells originating from a human colon carcinoma. At 37 degrees and in complete medium, the binding of [125I]insulin (1-4x10-10M) reaches a maximum in 40 min and the cell associated radioactivity remains constant for at least 4 h. No degradation of the hormone is observed under these conditions. The binding is proportional to the number of cells and its pH optimum is 7.8. In the presence of excess insulin 50% of the [125I]insulin is dissociated from the complex after 10 min. At equilibrium, insulin binding is specific: proinsulin is 25 times less potent than native insulin in competing with [125I]insulin and related polypeptide hormones are inactive. Scatchard analysis indicates two classes of binding sites (1400 sites/cell of \"high affinity\" e.g. 4.7 x 108 M-1, and 20 000 sites of \"low affinity\" e.g. 4 x 107 M-1). The binding of insulin to this non-target cell shows the same kinetic characteristics and specificity as found for insulin in its target cells, except that HT 29 cells do not degrade the hormone. The problem of the correlation between insulin binding and a biological effect in these cells remains to be elucidated.", "contents": "Insulin binding by a cell line (HT 29) derived from human colonic cancer. Insulin binding was demonstrated in cultured HT 29 cells originating from a human colon carcinoma. At 37 degrees and in complete medium, the binding of [125I]insulin (1-4x10-10M) reaches a maximum in 40 min and the cell associated radioactivity remains constant for at least 4 h. No degradation of the hormone is observed under these conditions. The binding is proportional to the number of cells and its pH optimum is 7.8. In the presence of excess insulin 50% of the [125I]insulin is dissociated from the complex after 10 min. At equilibrium, insulin binding is specific: proinsulin is 25 times less potent than native insulin in competing with [125I]insulin and related polypeptide hormones are inactive. Scatchard analysis indicates two classes of binding sites (1400 sites/cell of \"high affinity\" e.g. 4.7 x 108 M-1, and 20 000 sites of \"low affinity\" e.g. 4 x 107 M-1). The binding of insulin to this non-target cell shows the same kinetic characteristics and specificity as found for insulin in its target cells, except that HT 29 cells do not degrade the hormone. The problem of the correlation between insulin binding and a biological effect in these cells remains to be elucidated."}
{"id": "PMID:467780", "title": "Melatonin-and serotonin-stimulated release of vasopressin from rat neurohypophysis in vitro.", "content": "In order to study the control of vasopressin-release, the effect of a series of potential agents was studied in an in vitro perifusion system of rat neurohypophysis after in vivo treatment with nialamide, a monoamine oxidase inhibitor. In this system, metlatonin stimulated vasopressin-release in a dose-dependent manner (1 x 10-8 to 1 x 10-3 M). Serotonin (1 x 10-3 M) also led to a significant increase of vasopressin-release whereas quipazine (1 x 10-3 M), a putative serotonin agonist and monoamine oxidase inhibitor, caused a 3-fold stimulation of the release of the neurohormone. The stimulatory effects of melatonin and serotonin were prevented by omission of Ca2+ combined to an excess of Mg2+ (12mM) in the perifusion medium. 1 x 10-6 M somatostatin did not affect basal or melatonin-stimulated vasopressin-release. These results show that melatonin and serotonin can have a direct stimulatory effect on vasopressin release at the neurohypophyseal level.", "contents": "Melatonin-and serotonin-stimulated release of vasopressin from rat neurohypophysis in vitro. In order to study the control of vasopressin-release, the effect of a series of potential agents was studied in an in vitro perifusion system of rat neurohypophysis after in vivo treatment with nialamide, a monoamine oxidase inhibitor. In this system, metlatonin stimulated vasopressin-release in a dose-dependent manner (1 x 10-8 to 1 x 10-3 M). Serotonin (1 x 10-3 M) also led to a significant increase of vasopressin-release whereas quipazine (1 x 10-3 M), a putative serotonin agonist and monoamine oxidase inhibitor, caused a 3-fold stimulation of the release of the neurohormone. The stimulatory effects of melatonin and serotonin were prevented by omission of Ca2+ combined to an excess of Mg2+ (12mM) in the perifusion medium. 1 x 10-6 M somatostatin did not affect basal or melatonin-stimulated vasopressin-release. These results show that melatonin and serotonin can have a direct stimulatory effect on vasopressin release at the neurohypophyseal level."}
{"id": "PMID:467781", "title": "Antidopaminergic activity of estrogens on prolactin release at the pituitary level in vivo.", "content": "Treatment of female or male rats with estradiol benzoate led to an almost complete reversal of the inhibitory effect of low doses of dopamine on prolactin secretion. These data indicate that estrogens which have previously been shown to exert a potent antidopaminergic activity on prolactin secretion in anterior pituitary cells in primary culture have similar effects in vivo.", "contents": "Antidopaminergic activity of estrogens on prolactin release at the pituitary level in vivo. Treatment of female or male rats with estradiol benzoate led to an almost complete reversal of the inhibitory effect of low doses of dopamine on prolactin secretion. These data indicate that estrogens which have previously been shown to exert a potent antidopaminergic activity on prolactin secretion in anterior pituitary cells in primary culture have similar effects in vivo."}
{"id": "PMID:467794", "title": "Communicating vessel systems for mass cell culture of anchorage dependent cells.", "content": "Simultaneous filling of a number of vessels with identical input dosage is possible through an operation which uses the principle of communicating vessels with or without air pressure equilibration (APE). Sets of Roux flaska without APE and the NUNC Multitray Unit system with APE are presented. The latter system is used for large scale propagation of human diploid fibroblasts for production of large quantities of interferon.", "contents": "Communicating vessel systems for mass cell culture of anchorage dependent cells. Simultaneous filling of a number of vessels with identical input dosage is possible through an operation which uses the principle of communicating vessels with or without air pressure equilibration (APE). Sets of Roux flaska without APE and the NUNC Multitray Unit system with APE are presented. The latter system is used for large scale propagation of human diploid fibroblasts for production of large quantities of interferon."}
{"id": "PMID:467795", "title": "Serially-propagated human diploid cells: a synopsis of the present position concerning their use or producing viral vaccines and interferon.", "content": "Diploid cells of human origin subjected to serial propagation are being used on a wide and increasing scale as substrates for producing viral vaccines for use in humans. To a lesser extent they are also being used for the production of human interferon. A brief outline of the present position on the use of these cells for producing human vaccines together with information concerning their use for the production of human interferon is presented.", "contents": "Serially-propagated human diploid cells: a synopsis of the present position concerning their use or producing viral vaccines and interferon. Diploid cells of human origin subjected to serial propagation are being used on a wide and increasing scale as substrates for producing viral vaccines for use in humans. To a lesser extent they are also being used for the production of human interferon. A brief outline of the present position on the use of these cells for producing human vaccines together with information concerning their use for the production of human interferon is presented."}
{"id": "PMID:467798", "title": "Use of a new bead microcarrier for the culture of anchorage dependent cells in pseudo suspension.", "content": "The use of cross linked dextran beads having a surface charge produced using the method of Levine et al. (1976) is described. The new microcarriers require no pretreatment, and may be used at greater concentrations than those originally described by van Wezel (1967). Microcarrier cultures are initiated by inoculating cells into a stirred suspension of beads in culture medium. The cells spread over the surfaces of the beads, and follow typical growth curves. They may be harvested by conventional trypsinisation, and separated from denuded beads using a sintered glass filter. The beads may be regenerated and re-used. The beads have been found to be suitable for the growth of a variety of fibroblastoic cell types. It is suggested that the microcarriers are suited for bulk virus or metabolitic production from anchorage dependent cells.", "contents": "Use of a new bead microcarrier for the culture of anchorage dependent cells in pseudo suspension. The use of cross linked dextran beads having a surface charge produced using the method of Levine et al. (1976) is described. The new microcarriers require no pretreatment, and may be used at greater concentrations than those originally described by van Wezel (1967). Microcarrier cultures are initiated by inoculating cells into a stirred suspension of beads in culture medium. The cells spread over the surfaces of the beads, and follow typical growth curves. They may be harvested by conventional trypsinisation, and separated from denuded beads using a sintered glass filter. The beads may be regenerated and re-used. The beads have been found to be suitable for the growth of a variety of fibroblastoic cell types. It is suggested that the microcarriers are suited for bulk virus or metabolitic production from anchorage dependent cells."}
{"id": "PMID:467799", "title": "Parameters affecting cell growth on reduced charge microcarriers.", "content": "The development of the reduced charge microcarrier and cell behavior on carriers of different degrees of DEAE substitution, and under different growth conditions are described. Parameters other than the microcarrier which limit productivity are discussed, and microcarrier growth data for certain cell types are presented.", "contents": "Parameters affecting cell growth on reduced charge microcarriers. The development of the reduced charge microcarrier and cell behavior on carriers of different degrees of DEAE substitution, and under different growth conditions are described. Parameters other than the microcarrier which limit productivity are discussed, and microcarrier growth data for certain cell types are presented."}
{"id": "PMID:467801", "title": "Biological and biochemical criteria for the characterization of the transformed stage of BHK21 cells.", "content": "Some quantitative criteria for spontaneous and virus-induced transformation of BHK21 cells are described. The resistance to acid polysaccharides such as dextran sulphate in agarose medium is a characteristic shared by all tumorigenic clones. The increase in a high molecular weight integral membrane protein seems to be a biochemical feature correlated with this property.", "contents": "Biological and biochemical criteria for the characterization of the transformed stage of BHK21 cells. Some quantitative criteria for spontaneous and virus-induced transformation of BHK21 cells are described. The resistance to acid polysaccharides such as dextran sulphate in agarose medium is a characteristic shared by all tumorigenic clones. The increase in a high molecular weight integral membrane protein seems to be a biochemical feature correlated with this property."}
{"id": "PMID:467802", "title": "Biochemical aspects of muscle differentiation as analyzed by in vitro cultivation techniques.", "content": "Myoblasts cultivated in vitro will undergo terminal differentiation to form muscle fibres. Teratoma derived mouse cell lines, a pluripotent primitive line, and a muscle cell line, provide a possibility for comparing RNA populations in an early precursor cell with those in committed myoblasts and differentiated myotubes. Molecular hybridization analyses led to the conclusion that new RNA sequences appear in the cytoplasm during muscle differentiation. Such muscle specific sequences are not detectable in the nuclear RNA of myoblasts or primitive cells. Studies of protein synthesis during terminal myogenesis indicate co-ordinate expression of the muscle contractile proteins. These represent distinct isozymes, distinguishable from the contractile proteins of other cell types. In the case of myosin light chains isozymic transitions between different muscle forms have been identified during early development.", "contents": "Biochemical aspects of muscle differentiation as analyzed by in vitro cultivation techniques. Myoblasts cultivated in vitro will undergo terminal differentiation to form muscle fibres. Teratoma derived mouse cell lines, a pluripotent primitive line, and a muscle cell line, provide a possibility for comparing RNA populations in an early precursor cell with those in committed myoblasts and differentiated myotubes. Molecular hybridization analyses led to the conclusion that new RNA sequences appear in the cytoplasm during muscle differentiation. Such muscle specific sequences are not detectable in the nuclear RNA of myoblasts or primitive cells. Studies of protein synthesis during terminal myogenesis indicate co-ordinate expression of the muscle contractile proteins. These represent distinct isozymes, distinguishable from the contractile proteins of other cell types. In the case of myosin light chains isozymic transitions between different muscle forms have been identified during early development."}
{"id": "PMID:467803", "title": "Isolation of biologically active components from rabies and other envelope viruses.", "content": "Most human virus vaccines contain complete virus particles, either inactivated or attenuated. Besides components responsible for induction of neutralizing antibodies, other virus components (e.g. nucleic acids, lipids) are also administered upon vaccination. For envelope viruses the (glyco) proteins of the viral envelope are generally involved in the induction of neutralizing antibodies. Our investigations are focussed on the large scale preparation of these components from several viruses or virus vaccines, such as rabies and influenza. For virus disintegration we have tested several ionic anc nonionic detergents. Triton X-100 gave good results. Separation of solubilized components from the remainder of the virus has been carried out on a small scale by ultracentrifugation. For the purification of influenza hemagglutinin and neuraminidase we also used gelfiltration with success. The latter process can be scaled up easily. The main problem in the process of virus subunit preparation is the removal of detergent.", "contents": "Isolation of biologically active components from rabies and other envelope viruses. Most human virus vaccines contain complete virus particles, either inactivated or attenuated. Besides components responsible for induction of neutralizing antibodies, other virus components (e.g. nucleic acids, lipids) are also administered upon vaccination. For envelope viruses the (glyco) proteins of the viral envelope are generally involved in the induction of neutralizing antibodies. Our investigations are focussed on the large scale preparation of these components from several viruses or virus vaccines, such as rabies and influenza. For virus disintegration we have tested several ionic anc nonionic detergents. Triton X-100 gave good results. Separation of solubilized components from the remainder of the virus has been carried out on a small scale by ultracentrifugation. For the purification of influenza hemagglutinin and neuraminidase we also used gelfiltration with success. The latter process can be scaled up easily. The main problem in the process of virus subunit preparation is the removal of detergent."}
{"id": "PMID:467807", "title": "Training adaptations in skeletal muscle of juvenile diabetics.", "content": "Skeletal muscles from 12 male, juvenile-onset diabetics (JD) and 13 nondiabetics (ND) were studied to determine the effects of endurance training on mitochondrial enzyme activities, lipoprotein lipase (LPL) activity, and the oxidation of lipids (14C-palmityl CoA) in vitro. Ten weeks of endurance running (30 min/day, 5 days/wk) resulted in 11.0 and 12.9% gains in aerobic capacity for the JD and ND groups (P greater than 0.05), respectively. Both groups showed significant (P less than 0.05) increases in muscle LPL, carnitine palmityl transferase, succinate dehydrogenase, and hexokinase activities with training. Though the pretraining capacities for 14C-palmityl CoA oxidation were similar for both ND and JD groups, the diabetics showed a 41% greater improvement in the measurement of muscle lipid oxidation after training than did the ND group. The principal finding of this research was that skeletal muscle of juvenile diabetics who are in moderate insulin balance shows adaptations to endurance training that are similar to those of nondiabetic men.", "contents": "Training adaptations in skeletal muscle of juvenile diabetics. Skeletal muscles from 12 male, juvenile-onset diabetics (JD) and 13 nondiabetics (ND) were studied to determine the effects of endurance training on mitochondrial enzyme activities, lipoprotein lipase (LPL) activity, and the oxidation of lipids (14C-palmityl CoA) in vitro. Ten weeks of endurance running (30 min/day, 5 days/wk) resulted in 11.0 and 12.9% gains in aerobic capacity for the JD and ND groups (P greater than 0.05), respectively. Both groups showed significant (P less than 0.05) increases in muscle LPL, carnitine palmityl transferase, succinate dehydrogenase, and hexokinase activities with training. Though the pretraining capacities for 14C-palmityl CoA oxidation were similar for both ND and JD groups, the diabetics showed a 41% greater improvement in the measurement of muscle lipid oxidation after training than did the ND group. The principal finding of this research was that skeletal muscle of juvenile diabetics who are in moderate insulin balance shows adaptations to endurance training that are similar to those of nondiabetic men."}
{"id": "PMID:467808", "title": "Opposite effects of insulin and glucagon in acute hormonal control of hepatic lipogenesis.", "content": "Conditions for the isolation of rat hepatocytes that are responsive to insulin with regard to fatty acid synthesis were explored. Cells prepared according to the procedure of Ingebretsen and Wagle require the presence of fetal calf serum for insulin expression. Cells isolated by the Seglen method are the preparation of choice, since they respond to insulin in a simple, well-defined medium and, moreover, show much higher basal rates of fatty acid synthesis. In the latter cells isolated from fed male rats, the rate of fatty acid synthesis, as determined by tritium incorporation from [3H]H2O at 37 degrees C, is enhanced within 30 min after addition of insulin to the incubation medium; with glucagon, it is depressed. In the presence of insulin, the cellular content of malonyl coenzyme A is noticeably increased, whereas the concentrations of pyruvate, lactate, and citrate are not markedly affected. Glucagon, on the other hand, decreases the concentrations of all four intermediates. The activity of acetyl-CoA carboxylase is stimulated and depressed after addition of insulin and glucagon, respectively. In all conditions tested, the activity of acetyl-CoA carboxylase correlates with the rate of fatty acid synthesis, which in turn correlates with the cellular level of malonyl-CoA.", "contents": "Opposite effects of insulin and glucagon in acute hormonal control of hepatic lipogenesis. Conditions for the isolation of rat hepatocytes that are responsive to insulin with regard to fatty acid synthesis were explored. Cells prepared according to the procedure of Ingebretsen and Wagle require the presence of fetal calf serum for insulin expression. Cells isolated by the Seglen method are the preparation of choice, since they respond to insulin in a simple, well-defined medium and, moreover, show much higher basal rates of fatty acid synthesis. In the latter cells isolated from fed male rats, the rate of fatty acid synthesis, as determined by tritium incorporation from [3H]H2O at 37 degrees C, is enhanced within 30 min after addition of insulin to the incubation medium; with glucagon, it is depressed. In the presence of insulin, the cellular content of malonyl coenzyme A is noticeably increased, whereas the concentrations of pyruvate, lactate, and citrate are not markedly affected. Glucagon, on the other hand, decreases the concentrations of all four intermediates. The activity of acetyl-CoA carboxylase is stimulated and depressed after addition of insulin and glucagon, respectively. In all conditions tested, the activity of acetyl-CoA carboxylase correlates with the rate of fatty acid synthesis, which in turn correlates with the cellular level of malonyl-CoA."}
{"id": "PMID:467810", "title": "Acetylsalicyclic acid restores acute insulin response reduced by furosemide in man.", "content": "Prostaglandin E (PGE) infusion in normal man inhibits the acute insulin response to glucose. In order to determine whether endogenously released PGE might also inhibit insulin secretion, glucose-stimulated insulin responses were investigated in normal volunteers after furosemide (40 mg i.v.), a stimulator of endogenous PGE synthesis. Acute insulin response to glucose (20 g i.v.) was significantly reduced by furosemide (response before furosemide: 36 +/- 5 muU/ml; after furosemide: 26 +/- 5 muU/ml, m +/- SE, mean change 3--10 min, N = 8, P less than 0.01), whereas glucose disappearance rates were not modified after furosemide. Infusion of lysine acetylsalicylate (LAS), an inhibitor of endogenous PGE synthesis, completely reversed the inhibitory effect of furosemide on insulin secretion and also augmented acute insulin response to glucose (response before furosemide + LAS: 41 +/- 6 muU/ml; during furosemide + LAS: 50 +/- 7 muU/ml, N = 10, P less than 0.02). This effect was associated with an increase in glucose disappearance rates (P less than 0.05). These findings demonstrate that (1) furosemide inhibits glucose-induced acute insulin responses and (2) LAS completely reverses the inhibitory effect of furosemide and also accelerates glucose disposal. It is suggested that furosemide acts via the release of endogenous PGEs, which are known to inhibit insulin responses in man.", "contents": "Acetylsalicyclic acid restores acute insulin response reduced by furosemide in man. Prostaglandin E (PGE) infusion in normal man inhibits the acute insulin response to glucose. In order to determine whether endogenously released PGE might also inhibit insulin secretion, glucose-stimulated insulin responses were investigated in normal volunteers after furosemide (40 mg i.v.), a stimulator of endogenous PGE synthesis. Acute insulin response to glucose (20 g i.v.) was significantly reduced by furosemide (response before furosemide: 36 +/- 5 muU/ml; after furosemide: 26 +/- 5 muU/ml, m +/- SE, mean change 3--10 min, N = 8, P less than 0.01), whereas glucose disappearance rates were not modified after furosemide. Infusion of lysine acetylsalicylate (LAS), an inhibitor of endogenous PGE synthesis, completely reversed the inhibitory effect of furosemide on insulin secretion and also augmented acute insulin response to glucose (response before furosemide + LAS: 41 +/- 6 muU/ml; during furosemide + LAS: 50 +/- 7 muU/ml, N = 10, P less than 0.02). This effect was associated with an increase in glucose disappearance rates (P less than 0.05). These findings demonstrate that (1) furosemide inhibits glucose-induced acute insulin responses and (2) LAS completely reverses the inhibitory effect of furosemide and also accelerates glucose disposal. It is suggested that furosemide acts via the release of endogenous PGEs, which are known to inhibit insulin responses in man."}
{"id": "PMID:467811", "title": "Variation in the disappearance of unlabeled insulin from plasma: studies with portal and peripheral infusions.", "content": "One hundred and thirty brief infusions of unlabeled insulin were given to 75 unanesthetized nondiabetic human beings. The patients were examined (1) by different doses of insulin (5--50 muU/kg), (2) by intraportal and peripheral infusion, (3) in the morning and in the afternoon, and (4) during normoglycemia and moderate, steady hyperglycemia. After intraportal infusion of insulin, the non-steady state plasma clearance rate (ml min(-1)kg(-1) was lower the larger the dose (decreased about 50% by a 10-fold increase in insulin dose) and lower at hyper- than at normoglycemia (decreased about 30% after small insulin doses). It was also lower the higher the relative body weight, but this correlation was weak. After peripheral insulin infusion, none of the above relationships was demonstrated. Regardless of the route of insulin infusion, plasma clearance rate did not vary with fasting plasma insulin concentration, time of day, insulin sensitivity, or glucose tolerance. We conclude that the liver modifies the distribution and removal of pancreatically released insulin in response to acute increases in insulin or glucose. Otherwise the plasma clearance rate of insulin showed a notable lack of relationship with common indexes of metabolism and with insulin action.", "contents": "Variation in the disappearance of unlabeled insulin from plasma: studies with portal and peripheral infusions. One hundred and thirty brief infusions of unlabeled insulin were given to 75 unanesthetized nondiabetic human beings. The patients were examined (1) by different doses of insulin (5--50 muU/kg), (2) by intraportal and peripheral infusion, (3) in the morning and in the afternoon, and (4) during normoglycemia and moderate, steady hyperglycemia. After intraportal infusion of insulin, the non-steady state plasma clearance rate (ml min(-1)kg(-1) was lower the larger the dose (decreased about 50% by a 10-fold increase in insulin dose) and lower at hyper- than at normoglycemia (decreased about 30% after small insulin doses). It was also lower the higher the relative body weight, but this correlation was weak. After peripheral insulin infusion, none of the above relationships was demonstrated. Regardless of the route of insulin infusion, plasma clearance rate did not vary with fasting plasma insulin concentration, time of day, insulin sensitivity, or glucose tolerance. We conclude that the liver modifies the distribution and removal of pancreatically released insulin in response to acute increases in insulin or glucose. Otherwise the plasma clearance rate of insulin showed a notable lack of relationship with common indexes of metabolism and with insulin action."}
{"id": "PMID:467812", "title": "The association between diabetic retinopathy and skeletal muscle capillary basal lamina thickening corrected for the influence of age and duration of diabetes.", "content": "The association between an objective measure of diabetic retinopathy and skeletal muscle capillary basal lamina thickness was examined in a group of 30 male insulin-treated diabetic subjects, mean age (+/- SD) 44.6 +/- 13.2 yr, duration of diabetes 21.2 +/- 11.2 yr, % ideal body weight (% IBW) 106 +/- 11%. In addition, muscle capillary basal lamina width was measured in a group of 18 nondiabeitc men, mean age 40.7 +/- 16.3 yr and % IBW 118 +/- 23%. The muscle capillary width of the diabetic subjects was significantly greater than that of the nondiabetic group (P less than 0.01), but the values of the two overlapped considerably. In the diabetic group, there was a significant association of basal lamina width with age (P less than 0.01) but not with duration of diabetes. The association between extent of retinopathy and muscle capillary basal lamina width was not strong. The findings of the study do not therefore support the use of an estimate of muscle capillary basal lamina thickness as a single representative measure of diabetic microangiopathy.", "contents": "The association between diabetic retinopathy and skeletal muscle capillary basal lamina thickening corrected for the influence of age and duration of diabetes. The association between an objective measure of diabetic retinopathy and skeletal muscle capillary basal lamina thickness was examined in a group of 30 male insulin-treated diabetic subjects, mean age (+/- SD) 44.6 +/- 13.2 yr, duration of diabetes 21.2 +/- 11.2 yr, % ideal body weight (% IBW) 106 +/- 11%. In addition, muscle capillary basal lamina width was measured in a group of 18 nondiabeitc men, mean age 40.7 +/- 16.3 yr and % IBW 118 +/- 23%. The muscle capillary width of the diabetic subjects was significantly greater than that of the nondiabetic group (P less than 0.01), but the values of the two overlapped considerably. In the diabetic group, there was a significant association of basal lamina width with age (P less than 0.01) but not with duration of diabetes. The association between extent of retinopathy and muscle capillary basal lamina width was not strong. The findings of the study do not therefore support the use of an estimate of muscle capillary basal lamina thickness as a single representative measure of diabetic microangiopathy."}
{"id": "PMID:467814", "title": "Developmental progression in play behavior of children between nine and thirty months. I: Spontaneous play and imitation.", "content": "The developmental progression of play behavior was studied cross-sectionally in 85 children at eight age-levels between nine and 30 months. In a structured play situation, three major play patterns were distinguished: (1) exploratory play (at the end of the first year); (2) functional play (beginning of second year); and (3) representational play (after 15 months). Sequential play first occurred at 21 months. Play behavior with spatial characteristics was also observed: relational and container play between nine and 15 months, and understanding of spatial relationships, stacking and grouping between 18 and 24 months. There was a significant relationship between spontaneous and imitative play behavior; the greatest affect was displayed when the demonstrated behaviors matched the child's developmental level of play.", "contents": "Developmental progression in play behavior of children between nine and thirty months. I: Spontaneous play and imitation. The developmental progression of play behavior was studied cross-sectionally in 85 children at eight age-levels between nine and 30 months. In a structured play situation, three major play patterns were distinguished: (1) exploratory play (at the end of the first year); (2) functional play (beginning of second year); and (3) representational play (after 15 months). Sequential play first occurred at 21 months. Play behavior with spatial characteristics was also observed: relational and container play between nine and 15 months, and understanding of spatial relationships, stacking and grouping between 18 and 24 months. There was a significant relationship between spontaneous and imitative play behavior; the greatest affect was displayed when the demonstrated behaviors matched the child's developmental level of play."}
{"id": "PMID:467815", "title": "Intellectual development and academic achievement of children treated early for phenylketonuria.", "content": "Twenty early-treated children with classical phenylketonuria (PKU), five early-treated children with variant PKU and seven untreated children with hyperphenylalinemia were compared with non-PKU family members in terms of intellectual development, and 14 school-age PKU children were also compared for academic achievement. For the early-treated children with classical PKU, mean IQ (98) was within the normal range, but nine of these 20 children had IQ scores more than 1SD below those of family members. There was a significant negative correlation between phenylalanine concentrations at one to four years of age and later measured intelligence in these early-treated children, but this was probably a consequence of poor dietary control in the early years. The early-treated children with variant PKU and those with hyperphenylalaninemia had IQ scores consistent with those of unaffected family members, but untreated children with variant PKU had scores significantly lower than their own early-treated siblings. Achievement scores of the early-treated PKU children were consistent with their intellectual ability: they and their non-PKU siblings had similar standard scores for reading and spelling, but arithmetic scores were significantly lower for the PKU children. Early-treated children whose diet had been discontinued had achievement scores in all subjects below those predicted from their IQS.", "contents": "Intellectual development and academic achievement of children treated early for phenylketonuria. Twenty early-treated children with classical phenylketonuria (PKU), five early-treated children with variant PKU and seven untreated children with hyperphenylalinemia were compared with non-PKU family members in terms of intellectual development, and 14 school-age PKU children were also compared for academic achievement. For the early-treated children with classical PKU, mean IQ (98) was within the normal range, but nine of these 20 children had IQ scores more than 1SD below those of family members. There was a significant negative correlation between phenylalanine concentrations at one to four years of age and later measured intelligence in these early-treated children, but this was probably a consequence of poor dietary control in the early years. The early-treated children with variant PKU and those with hyperphenylalaninemia had IQ scores consistent with those of unaffected family members, but untreated children with variant PKU had scores significantly lower than their own early-treated siblings. Achievement scores of the early-treated PKU children were consistent with their intellectual ability: they and their non-PKU siblings had similar standard scores for reading and spelling, but arithmetic scores were significantly lower for the PKU children. Early-treated children whose diet had been discontinued had achievement scores in all subjects below those predicted from their IQS."}
{"id": "PMID:467816", "title": "Incidence and aetiology of infantile spasms from 1960 to 1976: a population study in Finland.", "content": "This series of cases of infantile spasms comprised 107 children born between 1960 and 1976 in the county of Uusimaa, Finland. The incidence of the syndrome was 0.38 to 0.42/1000 live births and has remained almost unchanged during those years, although there have been changes in the size of different aetiological groups. The importance of prenatal and perinatal factors, and especially of neonatal hypoglycaemia, as causal factors has decreased, whereas the numbers of cases due to infections and malformations has increased. Compared with investigations in other countries, this Finnish series differs in the incidence of familial cases and congenital cytomegalovirus infection, and especially in the higher incidence of neonatal hypoglycaemia and the lower incidence of tuberous sclerosis.", "contents": "Incidence and aetiology of infantile spasms from 1960 to 1976: a population study in Finland. This series of cases of infantile spasms comprised 107 children born between 1960 and 1976 in the county of Uusimaa, Finland. The incidence of the syndrome was 0.38 to 0.42/1000 live births and has remained almost unchanged during those years, although there have been changes in the size of different aetiological groups. The importance of prenatal and perinatal factors, and especially of neonatal hypoglycaemia, as causal factors has decreased, whereas the numbers of cases due to infections and malformations has increased. Compared with investigations in other countries, this Finnish series differs in the incidence of familial cases and congenital cytomegalovirus infection, and especially in the higher incidence of neonatal hypoglycaemia and the lower incidence of tuberous sclerosis."}
{"id": "PMID:467817", "title": "Disturbance in parent-child relationship following preterm delivery.", "content": "Increasing concern is expressed that the psychosocial development of preterm infants may be hindered by a disturbance of parental attitudes following the initial period of specialised care. Attitudes of parents of 17 preterm infants were compared with those of parents of 17 full-term infants at a single semi-structured interview six to 20 months after the birth. The groups of parents were matched for parity and did not differ in their social, ethnic and educational backgrounds. There was evidence of some disturbance in parent-child relationships in the preterm group, consisting of delays in maternal attachment to the child, negative maternal perception of the child compared with expectation of an 'average' baby, and persistent parental anxiety about leaving the child with a baby-sitter. In addition, two preterm children had been abused or neglected. Parental reaction to a preterm birth is discussed and the need for adequate support to be given to parents in the early period following the birth is stressed.", "contents": "Disturbance in parent-child relationship following preterm delivery. Increasing concern is expressed that the psychosocial development of preterm infants may be hindered by a disturbance of parental attitudes following the initial period of specialised care. Attitudes of parents of 17 preterm infants were compared with those of parents of 17 full-term infants at a single semi-structured interview six to 20 months after the birth. The groups of parents were matched for parity and did not differ in their social, ethnic and educational backgrounds. There was evidence of some disturbance in parent-child relationships in the preterm group, consisting of delays in maternal attachment to the child, negative maternal perception of the child compared with expectation of an 'average' baby, and persistent parental anxiety about leaving the child with a baby-sitter. In addition, two preterm children had been abused or neglected. Parental reaction to a preterm birth is discussed and the need for adequate support to be given to parents in the early period following the birth is stressed."}
{"id": "PMID:467818", "title": "Standardisation of the Denver Developmental Screening Test for Cardiff children.", "content": "The items in the Denver Developmental Screening Test (DDST) have been restandardised on a random sample of 1547 Cardiff children aged up to six years. Some differences were found between the ages at which the Cardiff and Denver children achieved certain test items. Cardiff children were consistently slightly slower on gross motor items throughout the age-range, with the exception of three items, but were slightly more advanced on early language items below the age of 18 months and on some personal and social items. The implications of the findings for the use of the DDST in Britain are discussed.", "contents": "Standardisation of the Denver Developmental Screening Test for Cardiff children. The items in the Denver Developmental Screening Test (DDST) have been restandardised on a random sample of 1547 Cardiff children aged up to six years. Some differences were found between the ages at which the Cardiff and Denver children achieved certain test items. Cardiff children were consistently slightly slower on gross motor items throughout the age-range, with the exception of three items, but were slightly more advanced on early language items below the age of 18 months and on some personal and social items. The implications of the findings for the use of the DDST in Britain are discussed."}
{"id": "PMID:467819", "title": "Teaching intermittent self-catheterization to young children with myelodysplasia.", "content": "A new technique is described which enables children with neurogenic bladder secondary to myelodysplasia to learn self-catheterization prior to their school years. Inexpensive dolls, complete with male or female genitalia, and a specially designed mirror to magnify the female children's genitalia, were used with four children (three girls, one boy) with mental ages of five years. Each child successfully demonstrated self-catheterization within two days, and gradual progress toward complete independence has been promoted.", "contents": "Teaching intermittent self-catheterization to young children with myelodysplasia. A new technique is described which enables children with neurogenic bladder secondary to myelodysplasia to learn self-catheterization prior to their school years. Inexpensive dolls, complete with male or female genitalia, and a specially designed mirror to magnify the female children's genitalia, were used with four children (three girls, one boy) with mental ages of five years. Each child successfully demonstrated self-catheterization within two days, and gradual progress toward complete independence has been promoted."}
{"id": "PMID:467820", "title": "A social score for kwashiorkor: explaining the look in the child's eyes.", "content": "Following the observation that many children with kwashiorkor had a deprived look in their eyes, the guardians of 107 children with kwashiorkor and 111 controls were interviewed about their home life, in a survey made at New Mulago Hospital, Kampala, between 1969 and 1972. A child's age, sex and tribe were found to have an influence on the nutritional state, therefore 50 of each group were matched for these variables. Ten significant associations were found which distinguished kwashiorkor patients from the control group. Children with kwashiorkor were more likely to be attended by someone other than the mother; to have changed attendants when ill; to have a pregnant mother; and to have separated parents. Further to these, singletons of split partnerships were at special risk; breast-feeding had stopped; weaning was begun for bad reasons; a child was more often living away from the parents and had been sent away coincident with weaning. The fathers tended to be poor. It is suggested that these 10 factors could be used as a social scoring system in assessing the risk of incipient kwashiorkor.", "contents": "A social score for kwashiorkor: explaining the look in the child's eyes. Following the observation that many children with kwashiorkor had a deprived look in their eyes, the guardians of 107 children with kwashiorkor and 111 controls were interviewed about their home life, in a survey made at New Mulago Hospital, Kampala, between 1969 and 1972. A child's age, sex and tribe were found to have an influence on the nutritional state, therefore 50 of each group were matched for these variables. Ten significant associations were found which distinguished kwashiorkor patients from the control group. Children with kwashiorkor were more likely to be attended by someone other than the mother; to have changed attendants when ill; to have a pregnant mother; and to have separated parents. Further to these, singletons of split partnerships were at special risk; breast-feeding had stopped; weaning was begun for bad reasons; a child was more often living away from the parents and had been sent away coincident with weaning. The fathers tended to be poor. It is suggested that these 10 factors could be used as a social scoring system in assessing the risk of incipient kwashiorkor."}
{"id": "PMID:467844", "title": "Insulin secretion and pancreatic exocrine function in patients with chronic pancreatitis.", "content": "The relationship between insulin responses to oral glucose and pancreatic exocrine function were examined in 15 patients with chronic pancreatitis. Good correlations were found between the insulin responses and exocrine pancreatic function measured as the concentrations of pancreatic enzymes in duodenal juice after intravenous cholecystokinin-pancreazymin (CCK-PZ). There appears to be a roughly parallel loss of endocrine and exocrine function in the course of chronic pancreatitis.", "contents": "Insulin secretion and pancreatic exocrine function in patients with chronic pancreatitis. The relationship between insulin responses to oral glucose and pancreatic exocrine function were examined in 15 patients with chronic pancreatitis. Good correlations were found between the insulin responses and exocrine pancreatic function measured as the concentrations of pancreatic enzymes in duodenal juice after intravenous cholecystokinin-pancreazymin (CCK-PZ). There appears to be a roughly parallel loss of endocrine and exocrine function in the course of chronic pancreatitis."}
{"id": "PMID:467846", "title": "Interrelationships of glucose and insulin uptake by muscle of normal and diabetic man. Evidence of a difference in metabolism of endogenous and exogenous insulin.", "content": "A forearm perfusion technique was used to study glucose and insulin uptake by muscle. In normal subjects at glycaemic levels above 130 mg/100 ml, glucose uptake was independent of glucose concentration;it was directly related in insulin concentration but not to insulin uptake. In non-obese maturity-onset diabetic subjects, glucose uptake was dependent on glucose concentration and insulin uptake, but not on insulin concentration. In both groups there was a strong correlation between insulin concentration and insulin uptake; diabetics had a normal insulin uptake in relation to concentration. For a given change in insulin concentration the increase in glucose uptake was as great in diabetics as in controls, but the effect of insulin was mediated through a mechanism involving its uptake. Thus in the non-obese maturity-onset diabetic, forearm muscle is not insulin resistant. The apparent uptake of insulin measured by a radioimmunoassay in relation to its arterial concentration was lower and more variable for heterologous than for endogenous insulin. With a receptor assay the venous insulin concentrations were lower than with the immunoassay and differences in uptake between endogenous and exogenous insulin disappeared. It is concluded that in muscle exogenous insulin was less severely degraded than endogenous insulin.", "contents": "Interrelationships of glucose and insulin uptake by muscle of normal and diabetic man. Evidence of a difference in metabolism of endogenous and exogenous insulin. A forearm perfusion technique was used to study glucose and insulin uptake by muscle. In normal subjects at glycaemic levels above 130 mg/100 ml, glucose uptake was independent of glucose concentration;it was directly related in insulin concentration but not to insulin uptake. In non-obese maturity-onset diabetic subjects, glucose uptake was dependent on glucose concentration and insulin uptake, but not on insulin concentration. In both groups there was a strong correlation between insulin concentration and insulin uptake; diabetics had a normal insulin uptake in relation to concentration. For a given change in insulin concentration the increase in glucose uptake was as great in diabetics as in controls, but the effect of insulin was mediated through a mechanism involving its uptake. Thus in the non-obese maturity-onset diabetic, forearm muscle is not insulin resistant. The apparent uptake of insulin measured by a radioimmunoassay in relation to its arterial concentration was lower and more variable for heterologous than for endogenous insulin. With a receptor assay the venous insulin concentrations were lower than with the immunoassay and differences in uptake between endogenous and exogenous insulin disappeared. It is concluded that in muscle exogenous insulin was less severely degraded than endogenous insulin."}
{"id": "PMID:467847", "title": "Fasting and two-hour post-load glucose levels for the diagnosis of diabetes. The relationship between glucose levels and complications of diabetes in the Pima Indians.", "content": "The frequency distributions of both the fasting and two-hour post-load plasma glucose levels were bimodal in the Pima Indian population aged 25 years and over. The hyperglycaemic component of this distribution represents those with diabetes mellitus, as some 30 percent of this group had evidence of the specific vascular complications of the disease, whereas these abnormalities were virtually absent in those with lower glucose levels. The bimodal characteristics of the frequency distributions were utilized to define optimal criteria to separate those with and without diabetes. The sensitivity and specificity of these criteria for fasting and two-hour glucose levels were compared and were found to be similar. The fasting glucose determination, however, was more reproducible and stable, as well as being easier to obtain, indicating that it is the better measurement for diagnostic purposes. The optimal level for diagnosis of 7.5 mmol/l (136 mg/dl) for the fasting glucose and the equivalent two-hour value of 14 mmol/l (250 mg/dl), were higher than many previously recommended diagnostic levels. Nevertheless, there was no evidence that subjects with lower levels were at appreciable risk of developing the specific complications of diabetes. Subjects with impaired glucose tolerance (IGT), but without fasting hyperglycaemia, should not be diagnosed as having diabetes mellitus.", "contents": "Fasting and two-hour post-load glucose levels for the diagnosis of diabetes. The relationship between glucose levels and complications of diabetes in the Pima Indians. The frequency distributions of both the fasting and two-hour post-load plasma glucose levels were bimodal in the Pima Indian population aged 25 years and over. The hyperglycaemic component of this distribution represents those with diabetes mellitus, as some 30 percent of this group had evidence of the specific vascular complications of the disease, whereas these abnormalities were virtually absent in those with lower glucose levels. The bimodal characteristics of the frequency distributions were utilized to define optimal criteria to separate those with and without diabetes. The sensitivity and specificity of these criteria for fasting and two-hour glucose levels were compared and were found to be similar. The fasting glucose determination, however, was more reproducible and stable, as well as being easier to obtain, indicating that it is the better measurement for diagnostic purposes. The optimal level for diagnosis of 7.5 mmol/l (136 mg/dl) for the fasting glucose and the equivalent two-hour value of 14 mmol/l (250 mg/dl), were higher than many previously recommended diagnostic levels. Nevertheless, there was no evidence that subjects with lower levels were at appreciable risk of developing the specific complications of diabetes. Subjects with impaired glucose tolerance (IGT), but without fasting hyperglycaemia, should not be diagnosed as having diabetes mellitus."}
{"id": "PMID:467848", "title": "Continuous subcutaneous insulin infusion:good blood glucose control for up to 4 days.", "content": "Six insulin-dependent diabetics were studied on their conventional insulin treatment and during continuous, dual-rate, subcutaneous insulin infusion for periods of up to 4 days. Dabetic control, as assessed by mean plasma glucose, range of plasma glucose values, M-value or range of M-values was improved significantly in 5 patients (mean +/- SD plasma glucose concentration on final infusion day 6.9 +/- 1.3 mmol/l, versus 11.3 +/- 3.2 mmol/l on conventional treatment). Once a suitable insulin dose was established blood glucose control could be maintained by continuous subcutaneous insulin infusion using the same daily infusion rate without frequent adjustment. In some case this was less than the daily dose on the conventional treatment. However, glycaemic control in one \"brittle\" diabetic, with unpredictable swings in blood glucose on her normal regimen, was not improved by continuous subcutaneous insulin infusion. During the period tested there was no sepsis at the cannula implantation site and patients did not find the system uncomfortable or unduly inconvenient.", "contents": "Continuous subcutaneous insulin infusion:good blood glucose control for up to 4 days. Six insulin-dependent diabetics were studied on their conventional insulin treatment and during continuous, dual-rate, subcutaneous insulin infusion for periods of up to 4 days. Dabetic control, as assessed by mean plasma glucose, range of plasma glucose values, M-value or range of M-values was improved significantly in 5 patients (mean +/- SD plasma glucose concentration on final infusion day 6.9 +/- 1.3 mmol/l, versus 11.3 +/- 3.2 mmol/l on conventional treatment). Once a suitable insulin dose was established blood glucose control could be maintained by continuous subcutaneous insulin infusion using the same daily infusion rate without frequent adjustment. In some case this was less than the daily dose on the conventional treatment. However, glycaemic control in one \"brittle\" diabetic, with unpredictable swings in blood glucose on her normal regimen, was not improved by continuous subcutaneous insulin infusion. During the period tested there was no sepsis at the cannula implantation site and patients did not find the system uncomfortable or unduly inconvenient."}
{"id": "PMID:467849", "title": "Adrenal gland involvement in mice with hereditary obesity and diabetes mellitus. Morphological studies.", "content": "The contribution of the adrenal gland to the development of the spontaneous syndrome of obesity and diabetes in Yellow-KK (Y-KK) mice was studied. Six-month old Y-KK mice exhibited hyperadrenocorticism and adrenal cortex enlargement. Light microscopic morphometric studies of Y-KK adrenals revealed an expanded volume of the adrenal cortex resulting from hyperplasia of zona fasciculata and reticularis cells. Ultrastructural studies revealed fewer lipid droplets, increased numbers of mitochondria and a more extensively developed Golgi system with zona fasciculata and reticularis cells. This cytological evidence of enhanced steroid biosynthetic and secretory activity was consistent with increased levels of plasma immunoreactive corticosterone. Structural and functional abnormalities of Y-KK adrenals were preceded by the development of obesity, hyperglycaemia and hyperinsulinaemia. It is unlikely, therefore, that the adrenal plays a casual role in the syndrome's pathogenesis, although, hyperadrenocorticism may be in part responsible for an exacerbation of the observed phenomena.", "contents": "Adrenal gland involvement in mice with hereditary obesity and diabetes mellitus. Morphological studies. The contribution of the adrenal gland to the development of the spontaneous syndrome of obesity and diabetes in Yellow-KK (Y-KK) mice was studied. Six-month old Y-KK mice exhibited hyperadrenocorticism and adrenal cortex enlargement. Light microscopic morphometric studies of Y-KK adrenals revealed an expanded volume of the adrenal cortex resulting from hyperplasia of zona fasciculata and reticularis cells. Ultrastructural studies revealed fewer lipid droplets, increased numbers of mitochondria and a more extensively developed Golgi system with zona fasciculata and reticularis cells. This cytological evidence of enhanced steroid biosynthetic and secretory activity was consistent with increased levels of plasma immunoreactive corticosterone. Structural and functional abnormalities of Y-KK adrenals were preceded by the development of obesity, hyperglycaemia and hyperinsulinaemia. It is unlikely, therefore, that the adrenal plays a casual role in the syndrome's pathogenesis, although, hyperadrenocorticism may be in part responsible for an exacerbation of the observed phenomena."}
{"id": "PMID:467852", "title": "Seasonal onset of insulin dependent diabetes in relation to sex and age at onset.", "content": "All newly diagnosed insulin dependent diabetics presenting consecutively to the Diabetic Department of the Royal Infirmary from the City of Edinburgh or its environs, between the years 1964 and 1977, were analysed for sex (297 males, 205 females), age at diagnosis (range 10--75 years), month of diagnosis, duration of symptoms and month of symptomatic onset. Males aged 10--19 years showed a significant seasonal variation in diagnosis (p less than 0.025) with an increase in autumn and winter months which was not seen in females aged 10--19 years nor in patients of either sex aged more than 19 years at diagnosis. The duration of diabetic symptoms increased with increasing age at diagnosis in both males and females and was consistently greater in females than in males for each age group at diagnosis. When the month of symptomatic onset was considered in relation to sex and age at diagnosis, having excluded patients with duration of symptoms of more than three months, only males aged 10--19 years at diagnosis showed a significant seasonal variation in incidence (p less than 0.005).", "contents": "Seasonal onset of insulin dependent diabetes in relation to sex and age at onset. All newly diagnosed insulin dependent diabetics presenting consecutively to the Diabetic Department of the Royal Infirmary from the City of Edinburgh or its environs, between the years 1964 and 1977, were analysed for sex (297 males, 205 females), age at diagnosis (range 10--75 years), month of diagnosis, duration of symptoms and month of symptomatic onset. Males aged 10--19 years showed a significant seasonal variation in diagnosis (p less than 0.025) with an increase in autumn and winter months which was not seen in females aged 10--19 years nor in patients of either sex aged more than 19 years at diagnosis. The duration of diabetic symptoms increased with increasing age at diagnosis in both males and females and was consistently greater in females than in males for each age group at diagnosis. When the month of symptomatic onset was considered in relation to sex and age at diagnosis, having excluded patients with duration of symptoms of more than three months, only males aged 10--19 years at diagnosis showed a significant seasonal variation in incidence (p less than 0.005)."}
{"id": "PMID:467853", "title": "Blood glucose regulation using closed- and open-loop insulin delivery systems. II. Peripheral primed square wave infusions.", "content": "Glucose was infused into anaesthetized dogs before and after pancreatectomy. In the diabetics blood glucose was regulated first by closed-loop and then by open-loop insulin delivery schemes. Insulin requirements for the latter were determined by resolving the former into a sequence of 3 different infusion rates: during the baseline and recovery periods, basal insulin was delivered at 0.37 +/- 0.02 mU/kg/min, while during the 60 min glucose infusion (10 mg/kg/min) there was an 8 min infusion at 4.96 +/- 0.37 mU/kg/min and a 52 min component at 1.85 +/- 0.08 mU/kg/min. With the open-loop method under these highly standardized conditions glycaemia was similar to normal controls but IRI levels were significantly higher, 13.5 vs 8.0 microU/ml (p less than 0.05) in the baseline and recovery periods and 74 vs 25 microU/ml (p less than 0.05) during the glucose infusion. It was concluded that: constant normoglycaemia can be maintained in the basal state by a constant rate of peripheral insulin delivery but at rates resulting in peripheral hyperinsulinaemia; the glycaemic response to glucose infusion can be normalized by a two component waveform of insulin delivery; and the closed-loop method can serve as a useful guide in determining insulin requirements.", "contents": "Blood glucose regulation using closed- and open-loop insulin delivery systems. II. Peripheral primed square wave infusions. Glucose was infused into anaesthetized dogs before and after pancreatectomy. In the diabetics blood glucose was regulated first by closed-loop and then by open-loop insulin delivery schemes. Insulin requirements for the latter were determined by resolving the former into a sequence of 3 different infusion rates: during the baseline and recovery periods, basal insulin was delivered at 0.37 +/- 0.02 mU/kg/min, while during the 60 min glucose infusion (10 mg/kg/min) there was an 8 min infusion at 4.96 +/- 0.37 mU/kg/min and a 52 min component at 1.85 +/- 0.08 mU/kg/min. With the open-loop method under these highly standardized conditions glycaemia was similar to normal controls but IRI levels were significantly higher, 13.5 vs 8.0 microU/ml (p less than 0.05) in the baseline and recovery periods and 74 vs 25 microU/ml (p less than 0.05) during the glucose infusion. It was concluded that: constant normoglycaemia can be maintained in the basal state by a constant rate of peripheral insulin delivery but at rates resulting in peripheral hyperinsulinaemia; the glycaemic response to glucose infusion can be normalized by a two component waveform of insulin delivery; and the closed-loop method can serve as a useful guide in determining insulin requirements."}
{"id": "PMID:467854", "title": "Effect of a long acting glucagon selective somatostatin analogue on plasma glucose, insulin and glucagon levels in the anaesthetized rat during arginine infusion.", "content": "The effects of somatostatin and a long acting, glucagon selective somatostatin analog (des-Ala1Gly2[His4,5-D-TrP8]-somatostatin) used studied during arginine tolerance tests in normal anaesthetized rats. Arginine infusion in control animals resulted in a rapid increase in plasma insulin and glucagon, and an increase of 15 +/- 5 mg/dl in plasma glucose. Somatostatin infusion (1 mg/kg/h) resulted in suppression of basal insulin secretion and a decrease in arginine-induced insulin and glucagon release. Glucose levels increased rapidly during the combined arginine-somatostatin infusion reaching a peak of 72 +/- 10 mg/dl above basal levels. Similar results were obtained when somatostatin was injected SC (1 mg/kg) at times 0, 15, 30, and 45 minutes (arginine infused from 30-60 minutes). A single injection (1 mg/kg) of the long-acting somatostatin analogue resulted in significant inhibition of basal insulin and glucagon release; during arginine infusion glucagon levels rose only slightly, the insulin response was, however, nearly normal, and only a small arginine-induced increase in glucose levels was observed. Carbohydrate absorption was not influenced by either somatostatin or the analogue.", "contents": "Effect of a long acting glucagon selective somatostatin analogue on plasma glucose, insulin and glucagon levels in the anaesthetized rat during arginine infusion. The effects of somatostatin and a long acting, glucagon selective somatostatin analog (des-Ala1Gly2[His4,5-D-TrP8]-somatostatin) used studied during arginine tolerance tests in normal anaesthetized rats. Arginine infusion in control animals resulted in a rapid increase in plasma insulin and glucagon, and an increase of 15 +/- 5 mg/dl in plasma glucose. Somatostatin infusion (1 mg/kg/h) resulted in suppression of basal insulin secretion and a decrease in arginine-induced insulin and glucagon release. Glucose levels increased rapidly during the combined arginine-somatostatin infusion reaching a peak of 72 +/- 10 mg/dl above basal levels. Similar results were obtained when somatostatin was injected SC (1 mg/kg) at times 0, 15, 30, and 45 minutes (arginine infused from 30-60 minutes). A single injection (1 mg/kg) of the long-acting somatostatin analogue resulted in significant inhibition of basal insulin and glucagon release; during arginine infusion glucagon levels rose only slightly, the insulin response was, however, nearly normal, and only a small arginine-induced increase in glucose levels was observed. Carbohydrate absorption was not influenced by either somatostatin or the analogue."}
{"id": "PMID:467855", "title": "The relative contributions of newly synthesized and stored messages to Hl histone synthesis in interspecies hybrid echinoid embryos.", "content": "We have measured the ratio of incorporation of 3H-lysine into the maternal and paternal forms of Hl histones synthesized by the interordinal hybrid embryo which results from the fertilization of sand dollar eggs with sea urchin sperm. This ratio has been used to calculate the relative contributions of newly transcribed and stored Hl histones mRNA to the synthesis of Hl histone at five different stages of development. These calculations are based on the assumption that histone mRNA of both parental types is transcribed with equal efficiency from the genome and that these RNAs are translated with equal efficiency in the cytoplasm of the hybrid embryos. On this basis, we have estimated that the contribution of new mRNA represents 80% of total Hl histone synthesis at the 16--32 cell stage, 54% at the hatching blastula stage, 40% at the mesenchyme blastula stage, and 100% after gastrulation. These data are discussed in the light of presently known parameters of histone and histone mRNA synthesis in echinoderm embryos.", "contents": "The relative contributions of newly synthesized and stored messages to Hl histone synthesis in interspecies hybrid echinoid embryos. We have measured the ratio of incorporation of 3H-lysine into the maternal and paternal forms of Hl histones synthesized by the interordinal hybrid embryo which results from the fertilization of sand dollar eggs with sea urchin sperm. This ratio has been used to calculate the relative contributions of newly transcribed and stored Hl histones mRNA to the synthesis of Hl histone at five different stages of development. These calculations are based on the assumption that histone mRNA of both parental types is transcribed with equal efficiency from the genome and that these RNAs are translated with equal efficiency in the cytoplasm of the hybrid embryos. On this basis, we have estimated that the contribution of new mRNA represents 80% of total Hl histone synthesis at the 16--32 cell stage, 54% at the hatching blastula stage, 40% at the mesenchyme blastula stage, and 100% after gastrulation. These data are discussed in the light of presently known parameters of histone and histone mRNA synthesis in echinoderm embryos."}
{"id": "PMID:467856", "title": "Gel electrophoretic analysis of histones in lens epithelium, lens fiber, liver, brain, and erythrocytes of late chick embryos.", "content": "Histones from 19-day-old chick embryo lens epithelium, lens fibers, liver, brain, and erythrocytes were electrophoresed in polyacrylamide gels using buffers containing sodium dodecylsulfate, acetic acid urea, or mixtures of Triton X-100 acetic acid urea. In the last two buffer systems, histone bands were characterized by their apparent molecular weights determined by electrophoresis in the second dimension in sodium dodecylsulfate containing polyacrylamide gels. From the densitograms of the stained gels, the relative proportion of protein in different histone bands was estimated. With the exception of the erythrocyte-specific histone H5, all histones from different tissues examined at any of the gel systems migrated with the same mobilities. In lens epithelium and lens fibers, all histones were present in identical proportions. As compared to liver and brain, the total amount of histone Hl was significantly lower in lens cells and erythrocytes, possibly reflecting differences between the differentiated states. However, no tissue-specific differences were found in the relative distribution of histone Hl I and Hl II among lens epithelium, lens fiber, liver and, brain, but a threefold higher Hl I : Hl II ratio (0.5--0.7) was found in erythrocytes.", "contents": "Gel electrophoretic analysis of histones in lens epithelium, lens fiber, liver, brain, and erythrocytes of late chick embryos. Histones from 19-day-old chick embryo lens epithelium, lens fibers, liver, brain, and erythrocytes were electrophoresed in polyacrylamide gels using buffers containing sodium dodecylsulfate, acetic acid urea, or mixtures of Triton X-100 acetic acid urea. In the last two buffer systems, histone bands were characterized by their apparent molecular weights determined by electrophoresis in the second dimension in sodium dodecylsulfate containing polyacrylamide gels. From the densitograms of the stained gels, the relative proportion of protein in different histone bands was estimated. With the exception of the erythrocyte-specific histone H5, all histones from different tissues examined at any of the gel systems migrated with the same mobilities. In lens epithelium and lens fibers, all histones were present in identical proportions. As compared to liver and brain, the total amount of histone Hl was significantly lower in lens cells and erythrocytes, possibly reflecting differences between the differentiated states. However, no tissue-specific differences were found in the relative distribution of histone Hl I and Hl II among lens epithelium, lens fiber, liver and, brain, but a threefold higher Hl I : Hl II ratio (0.5--0.7) was found in erythrocytes."}
{"id": "PMID:467857", "title": "Sub-cellular distribution of the cytoplasmic myosin heavy chain mRNA during myogenesis.", "content": "In the light of earlier work [1] which demonstrated the presence of a large number of myosin heavy chain (MHC) transcripts in chick myoblasts prior to cell fusion and the burst of MHC synthesis it was of great interest to determine the subcellular localization of the still inactive transcripts. It has been determined in differentiating muscle cells in culture. Two populations of cells were examined -- monucleated myoblasts just prior to cell fusion and myotubes where at least 80% of the cells were fused. Utilizing a myosin complementary DNA (cDNA) probe [2] it is observed that just prior to cell fusion, when the \"burst\" of myosin synthesis has not yet occurred, the vast majority of cytoplasmic myosin mRNA transcripts are found in a stored messenger RNA protein complex with a minimal amount found in the heavy polysome fraction. In differentiated myotube cultures, when myosin synthesis is progressing at a high rate, the reverse is found, i.e, the amount of stored myosin messenger RNA (mRNA) is minimal while the largest amount of myosin mRNA transcripts are localized in the polysome fraction. The number of total cytoplasmic myosin transcripts is found to decrease after cell fusion at a time when myosin synthesis is maximal suggesting that the efficiency of translation of myosin mRNA increases during terminal differentiation.", "contents": "Sub-cellular distribution of the cytoplasmic myosin heavy chain mRNA during myogenesis. In the light of earlier work [1] which demonstrated the presence of a large number of myosin heavy chain (MHC) transcripts in chick myoblasts prior to cell fusion and the burst of MHC synthesis it was of great interest to determine the subcellular localization of the still inactive transcripts. It has been determined in differentiating muscle cells in culture. Two populations of cells were examined -- monucleated myoblasts just prior to cell fusion and myotubes where at least 80% of the cells were fused. Utilizing a myosin complementary DNA (cDNA) probe [2] it is observed that just prior to cell fusion, when the \"burst\" of myosin synthesis has not yet occurred, the vast majority of cytoplasmic myosin mRNA transcripts are found in a stored messenger RNA protein complex with a minimal amount found in the heavy polysome fraction. In differentiated myotube cultures, when myosin synthesis is progressing at a high rate, the reverse is found, i.e, the amount of stored myosin messenger RNA (mRNA) is minimal while the largest amount of myosin mRNA transcripts are localized in the polysome fraction. The number of total cytoplasmic myosin transcripts is found to decrease after cell fusion at a time when myosin synthesis is maximal suggesting that the efficiency of translation of myosin mRNA increases during terminal differentiation."}
{"id": "PMID:467858", "title": "Proteins of BrdU-dependent hamster cell lines as characterized by two-dimensional gel electrophoresis.", "content": "Cell lines which exhibit the BrdU-dependent phenotype (B4 and HAB) were studied with respect to BrdU-induced alterations in genetic expression by two-dimensional gel electrophoresis. A comparison of the proteins from the HAB cells, in which the DNA is 100% substituted by BrdU, to those of the unsubstituted parent line (3460) showed 55 protein alterations; the synthesis of 15 increased while that of the other 40 decreased. When 3460 cells were grown in BrdU such that their DNA was greater than 50% substituted, 27 protein changes could be detected; of these, the synthesis of 10 increased while that of 17 decreased. A comparison of all these changes in the various cell lines showed six which were common to the BrdU-substituted cell lines. The proteins from another Syrian hamster cell line, BHK-21 (C-13) and those of HAB cells grown in thymidine or BrdC were also examined on two-dimensional gels. Although BrdU has a dramatic effect on many cellular functions, relatively few changes in the pattern of protein synthesis could be detected in these cell lines, perhaps reflecting the specialized action of this analogue on particular cellular functions.", "contents": "Proteins of BrdU-dependent hamster cell lines as characterized by two-dimensional gel electrophoresis. Cell lines which exhibit the BrdU-dependent phenotype (B4 and HAB) were studied with respect to BrdU-induced alterations in genetic expression by two-dimensional gel electrophoresis. A comparison of the proteins from the HAB cells, in which the DNA is 100% substituted by BrdU, to those of the unsubstituted parent line (3460) showed 55 protein alterations; the synthesis of 15 increased while that of the other 40 decreased. When 3460 cells were grown in BrdU such that their DNA was greater than 50% substituted, 27 protein changes could be detected; of these, the synthesis of 10 increased while that of 17 decreased. A comparison of all these changes in the various cell lines showed six which were common to the BrdU-substituted cell lines. The proteins from another Syrian hamster cell line, BHK-21 (C-13) and those of HAB cells grown in thymidine or BrdC were also examined on two-dimensional gels. Although BrdU has a dramatic effect on many cellular functions, relatively few changes in the pattern of protein synthesis could be detected in these cell lines, perhaps reflecting the specialized action of this analogue on particular cellular functions."}
{"id": "PMID:467859", "title": "Telolysomes in cultured iris epithelial cells and in the TVI cell-line.", "content": "Iris epithelial cells of adult newts, which are fully differntiated melanocytes and non-dividing, become dedifferentiated and converted into lens cells when put in culture. A recent study shows that this dedifferentiation is based on an autophagic process which is associated with proliferation and mainly affects melanosomes. The present report shows that in primary culture of iris epithelial cells after the majority of melanosomes have disappeared, myelinoid bodies, which are interpreted to be telolysosomes of autophagic nature, appear in high frequencies. This suggest that in these cells autophagy persists after the loss of melanosomes. A possible connection of this type of autophagy with the differentation of lens fiber which occurs in this culture is discussed. In the TVI cell line which is believed to be derived from the same cell type, but devoid of melanosomes, similar myelinoid bodies are a characteristic cell component, suggesting that the tendency for autophagy is inherited in theis cell line.", "contents": "Telolysomes in cultured iris epithelial cells and in the TVI cell-line. Iris epithelial cells of adult newts, which are fully differntiated melanocytes and non-dividing, become dedifferentiated and converted into lens cells when put in culture. A recent study shows that this dedifferentiation is based on an autophagic process which is associated with proliferation and mainly affects melanosomes. The present report shows that in primary culture of iris epithelial cells after the majority of melanosomes have disappeared, myelinoid bodies, which are interpreted to be telolysosomes of autophagic nature, appear in high frequencies. This suggest that in these cells autophagy persists after the loss of melanosomes. A possible connection of this type of autophagy with the differentation of lens fiber which occurs in this culture is discussed. In the TVI cell line which is believed to be derived from the same cell type, but devoid of melanosomes, similar myelinoid bodies are a characteristic cell component, suggesting that the tendency for autophagy is inherited in theis cell line."}
{"id": "PMID:467870", "title": "Classes of proteins synthesized in oocytes, eggs, embryos, and differentiated tissues of Xenopus laevis.", "content": "Two-dimensional gel electrophoresis has been used to analyse protein synthesis in embryonic stages and in three differentiated tissues of Xenopus laevis. The patterns found in oocyte, unfertilized eggs, embryos shortly after fertilization and at progressively later stages of development have been characterized and compared with the patterns found in the brain, heart and liver of tadpoles. The results suggest that at least four classes of proteins can be recognized among the proteins synthesized, although other categories may exist. They also suggest that some proteins synthesized rapidly in the oocyte are likely to be synthesized in differentiated tissues as well, while proteins synthesized for the first time only after fertilization are much less likely to be synthesized in differentiated tissues.", "contents": "Classes of proteins synthesized in oocytes, eggs, embryos, and differentiated tissues of Xenopus laevis. Two-dimensional gel electrophoresis has been used to analyse protein synthesis in embryonic stages and in three differentiated tissues of Xenopus laevis. The patterns found in oocyte, unfertilized eggs, embryos shortly after fertilization and at progressively later stages of development have been characterized and compared with the patterns found in the brain, heart and liver of tadpoles. The results suggest that at least four classes of proteins can be recognized among the proteins synthesized, although other categories may exist. They also suggest that some proteins synthesized rapidly in the oocyte are likely to be synthesized in differentiated tissues as well, while proteins synthesized for the first time only after fertilization are much less likely to be synthesized in differentiated tissues."}
{"id": "PMID:467871", "title": "Cell-free cytoplasmic polyadenylation of oogenic RNA.", "content": "The products of cell-free ATP incorporation mediated by cytoplasmic fractions prepared from unfertilized sea urchin eggs, anucleate egg halves, nucleate egg halves, emetine-treated fertilized eggs, and four-cell embryos have been characterized to determine to what extent the polymers synthesized are poly(A) and to assess the size distribution of the primers adenylated. As judged by alkaline lability, ribonuclease resistance, and retention on poly(U)-impregnated filters, greater than 92% of the label recovered after RNA extraction is present in poly(A). LiCl fractionation indicates that little, if any, free poly(A) is synthesized or cleaved from RNA primers during the reaction, and that 4S RNA is not an effective initiator. In excess of 85% of the poly(A) is associated with RNA having S-values greater than or equal to 18S. Sedimentation profiles of RNA adenylated in the unfertilized egg and anucleate egg half reactions are identical. Suppression of in vivo protein synthesis by emetine alters the profile of RNA subsequently adenylated in vitro. It is proposed that the apparent constraints on the utilization of cytoplasmic RNA or ribonucleoprotein primers of oogenic origin may be effected by RNA-associated proteins capable of regulating the selection and/or extent of their polyadenylation during early embryogenesis.", "contents": "Cell-free cytoplasmic polyadenylation of oogenic RNA. The products of cell-free ATP incorporation mediated by cytoplasmic fractions prepared from unfertilized sea urchin eggs, anucleate egg halves, nucleate egg halves, emetine-treated fertilized eggs, and four-cell embryos have been characterized to determine to what extent the polymers synthesized are poly(A) and to assess the size distribution of the primers adenylated. As judged by alkaline lability, ribonuclease resistance, and retention on poly(U)-impregnated filters, greater than 92% of the label recovered after RNA extraction is present in poly(A). LiCl fractionation indicates that little, if any, free poly(A) is synthesized or cleaved from RNA primers during the reaction, and that 4S RNA is not an effective initiator. In excess of 85% of the poly(A) is associated with RNA having S-values greater than or equal to 18S. Sedimentation profiles of RNA adenylated in the unfertilized egg and anucleate egg half reactions are identical. Suppression of in vivo protein synthesis by emetine alters the profile of RNA subsequently adenylated in vitro. It is proposed that the apparent constraints on the utilization of cytoplasmic RNA or ribonucleoprotein primers of oogenic origin may be effected by RNA-associated proteins capable of regulating the selection and/or extent of their polyadenylation during early embryogenesis."}
{"id": "PMID:467872", "title": "Mitosis in milk secreting epithelial cells of mammary gland: an ultrastructural study.", "content": "In all stages of lactation mitotic configurations were observed in mammary gland epithelial cells of rats. An electron microscopic study is presented which shows that ultrastructure of such mitotic stages is normal and that mitotic cells contain typical products of milk secreting cells such as casein micelle-containing vesicles and milk fat droplets. Such secretory products can even be observed in the immediate vicinity of the chromosomes and microtubules of the spindle apparatus. The endoplasmic reticulum of mitotic cells appeared altered in that it did not show typical cisternal stacks characteristic of interphase cells. While the numbers of such mitotic cells were very low, especially from the second week of lactation on (always less than 0.1% of the milk secreting epithelial cells encountered), the observations clearly demonstrate that differentiation for milk secretory activity and cells division are not mutually exclusive. We conclude that postpartum growth of mammary gland epithelium and replacement of epithelial cells lost during desquamation into the milk liquids can occur by division of existing differentiated milk secreting cells and does not require mitotic activity of non-lactating 'stem cells' which are not observed in lactating alveoli.", "contents": "Mitosis in milk secreting epithelial cells of mammary gland: an ultrastructural study. In all stages of lactation mitotic configurations were observed in mammary gland epithelial cells of rats. An electron microscopic study is presented which shows that ultrastructure of such mitotic stages is normal and that mitotic cells contain typical products of milk secreting cells such as casein micelle-containing vesicles and milk fat droplets. Such secretory products can even be observed in the immediate vicinity of the chromosomes and microtubules of the spindle apparatus. The endoplasmic reticulum of mitotic cells appeared altered in that it did not show typical cisternal stacks characteristic of interphase cells. While the numbers of such mitotic cells were very low, especially from the second week of lactation on (always less than 0.1% of the milk secreting epithelial cells encountered), the observations clearly demonstrate that differentiation for milk secretory activity and cells division are not mutually exclusive. We conclude that postpartum growth of mammary gland epithelium and replacement of epithelial cells lost during desquamation into the milk liquids can occur by division of existing differentiated milk secreting cells and does not require mitotic activity of non-lactating 'stem cells' which are not observed in lactating alveoli."}
{"id": "PMID:467883", "title": "Induction of intestinal metaplasia and carcinoma in the glandular stomach of rats by N-alkyl-N'-nitro-N-nitrosoguanidines.", "content": "Induction of intestinal metaplasia and carcinoma of the stomach in Wistar rats by four homologs of N-alkyl-N'-nitro-N-nitrosoguanidine (N-alkyl-NNG) was investigated. N-Propyl-, N-butyl, N-isobutyl, and N-pentyl-NNG's were administered to rats as 0.34mM solutions in the drinking water for 12 months and the rats were killed about 6 months later. Intestinal metaplasia was found in the glandular stomach of 100%, 50%, 44%, and 17% of the rats treated with N-propyl-, N-butyl-, N-isobutyl-, and N-pentyl-NNG's, respectively. Adenocarcinoma of the glandular stomach was found in 29% of the rats treated with N-propyl-NNG, but not in those treated with other N-alkyl-NNG's. In the control group, 11% of the rats had intestinal metaplasia but none had tumors.", "contents": "Induction of intestinal metaplasia and carcinoma in the glandular stomach of rats by N-alkyl-N'-nitro-N-nitrosoguanidines. Induction of intestinal metaplasia and carcinoma of the stomach in Wistar rats by four homologs of N-alkyl-N'-nitro-N-nitrosoguanidine (N-alkyl-NNG) was investigated. N-Propyl-, N-butyl, N-isobutyl, and N-pentyl-NNG's were administered to rats as 0.34mM solutions in the drinking water for 12 months and the rats were killed about 6 months later. Intestinal metaplasia was found in the glandular stomach of 100%, 50%, 44%, and 17% of the rats treated with N-propyl-, N-butyl-, N-isobutyl-, and N-pentyl-NNG's, respectively. Adenocarcinoma of the glandular stomach was found in 29% of the rats treated with N-propyl-NNG, but not in those treated with other N-alkyl-NNG's. In the control group, 11% of the rats had intestinal metaplasia but none had tumors."}
{"id": "PMID:467884", "title": "A new parameter for discriminating malignantly transformed cell lines from nontransformed counterparts: culture in liquid medium containing neutral protease.", "content": "Growth properties of malignantly transformed cell lines and their nontransformed counterparts were studied in the presence of a bacterial neutral protease (Dispase I). Among 10 transformed cell lines, 7 lines could proliferate in the presence of the protease in a floating state. Nontransformed cell lines and non-established cell strains failed to grow in this culture condition. The ability to grow in the presence of the protease was well correlated with their ability for anchorage-independent growth and tumorigenicity, and this provides a convenient primary test for evaluating cellular tumorigenicity.", "contents": "A new parameter for discriminating malignantly transformed cell lines from nontransformed counterparts: culture in liquid medium containing neutral protease. Growth properties of malignantly transformed cell lines and their nontransformed counterparts were studied in the presence of a bacterial neutral protease (Dispase I). Among 10 transformed cell lines, 7 lines could proliferate in the presence of the protease in a floating state. Nontransformed cell lines and non-established cell strains failed to grow in this culture condition. The ability to grow in the presence of the protease was well correlated with their ability for anchorage-independent growth and tumorigenicity, and this provides a convenient primary test for evaluating cellular tumorigenicity."}
{"id": "PMID:467885", "title": "Chronic toxicity of sodium nitrite in mice, with reference to its tumorigenicity.", "content": "Sodium nitrite has been widely used as one of the most effective food additives to tinge color on cured meat. However, it has been elucidated that this chemical is not merely a precursor of N-nitroso compounds, many of which are strongly carcinogenic, but also a mutagenic substance in biological tests. In order to ascertain the possible tumorigenicity of sodium nitrite itself, chronic toxicity of the agent in mice, by means of daily oral administration as drinking water for more than 18 months, in the concentration of 0.5 (maximum tolerated dose), 0.25, and 0.125%, was tested. As a result, development of various tumors, including thymic lymphoma, nonthymic lymphoid leukemia, pulmonary adenoma and carcinoma, and benign and malignant tumors in soft tissue, was seen in these mice. However, as to the incidence of tumors as well as the developmental time of each histologically classified tumor, no apparent difference was detected between those in the experimental groups and the control group.", "contents": "Chronic toxicity of sodium nitrite in mice, with reference to its tumorigenicity. Sodium nitrite has been widely used as one of the most effective food additives to tinge color on cured meat. However, it has been elucidated that this chemical is not merely a precursor of N-nitroso compounds, many of which are strongly carcinogenic, but also a mutagenic substance in biological tests. In order to ascertain the possible tumorigenicity of sodium nitrite itself, chronic toxicity of the agent in mice, by means of daily oral administration as drinking water for more than 18 months, in the concentration of 0.5 (maximum tolerated dose), 0.25, and 0.125%, was tested. As a result, development of various tumors, including thymic lymphoma, nonthymic lymphoid leukemia, pulmonary adenoma and carcinoma, and benign and malignant tumors in soft tissue, was seen in these mice. However, as to the incidence of tumors as well as the developmental time of each histologically classified tumor, no apparent difference was detected between those in the experimental groups and the control group."}
{"id": "PMID:467886", "title": "Effect of BCG on cytostatic activity of peritoneal macrophages from normal and tumor-bearing rats.", "content": "In tumor-bearing rats inoculated intramuscularly with 5 x 10(4) SLC cells, the cytostatic activity of peritoneal macrophages was elevated in the early stage (7 days after transplantation) and decreased in the advanced stage (21 days after transplantation). When BCG was intraperitoneally administered into normal and tumor-bearing rats, peritoneal macrophages showed higher cytostatic activity than the untreated macrophages. This elevated cytostatic activity of the marcophages obtained from BCG-treated tumor-bearing rats was maintained even at an advanced stage.", "contents": "Effect of BCG on cytostatic activity of peritoneal macrophages from normal and tumor-bearing rats. In tumor-bearing rats inoculated intramuscularly with 5 x 10(4) SLC cells, the cytostatic activity of peritoneal macrophages was elevated in the early stage (7 days after transplantation) and decreased in the advanced stage (21 days after transplantation). When BCG was intraperitoneally administered into normal and tumor-bearing rats, peritoneal macrophages showed higher cytostatic activity than the untreated macrophages. This elevated cytostatic activity of the marcophages obtained from BCG-treated tumor-bearing rats was maintained even at an advanced stage."}
{"id": "PMID:467887", "title": "A rapid screening for the specific DNA sequence: analysis of transforming DNA segments in adenovirus-transformed cells.", "content": "The viral DNA sequences in cells transformed by adenovirus type-12 (Ad12) DNA fragments were investigated by spot hybridization, a detection by autoradiography of nucleic acid hybrids formed between cell DNAs spotted on a membrane filter and various nick-translated Ad12 DNA fragments. In CY1 cell line, a rat cell line transformed by the EcoRI-C fragment (left hand 16%), all of the HindIII fragments included in the EcoRI-C fragment are shown to be present. In GY1 cell line, a rat cell line transformed by the HindIII-G fragment (left hand 7%), both of the BpaI-H and a part of the BpaI-J, two components consisting of the HindIII-G fragment, were found. A dominant presence of the Ad12 BpaI-H fragment (left hand end 4.5%) of the Ad12 DNA molecule, approximately 60% of the Ad12 transforming DNA sequences (Ad12 HindIII-G), was also shown in GY1. Spot hybridization used in the experiment will be of general use for detecting viral nucleic acid sequences in cells and provides a simple and useful screening method for investigating viral etiology of tumors and transformed cells.", "contents": "A rapid screening for the specific DNA sequence: analysis of transforming DNA segments in adenovirus-transformed cells. The viral DNA sequences in cells transformed by adenovirus type-12 (Ad12) DNA fragments were investigated by spot hybridization, a detection by autoradiography of nucleic acid hybrids formed between cell DNAs spotted on a membrane filter and various nick-translated Ad12 DNA fragments. In CY1 cell line, a rat cell line transformed by the EcoRI-C fragment (left hand 16%), all of the HindIII fragments included in the EcoRI-C fragment are shown to be present. In GY1 cell line, a rat cell line transformed by the HindIII-G fragment (left hand 7%), both of the BpaI-H and a part of the BpaI-J, two components consisting of the HindIII-G fragment, were found. A dominant presence of the Ad12 BpaI-H fragment (left hand end 4.5%) of the Ad12 DNA molecule, approximately 60% of the Ad12 transforming DNA sequences (Ad12 HindIII-G), was also shown in GY1. Spot hybridization used in the experiment will be of general use for detecting viral nucleic acid sequences in cells and provides a simple and useful screening method for investigating viral etiology of tumors and transformed cells."}
{"id": "PMID:467888", "title": "Lymphocyte responsiveness to phytohemagglutinin and its modification by serum inhibitor in breast cancer patients.", "content": "Lymphocyte responsiveness in stimulation with phytohemagglutinin (PHA) and the inhibitory effect of autologous serum on lymphocyte responsiveness were investigated in 55 patients with breast cancer. Lymphocytes from patients, even in the late stage of the disease, as well as from normal healthy control, showed an ability to respond to the stimulation with PHA in the presence of pooled normal serum, while lymphocyte responsiveness in the presence of autologous serum was markedly reduced in stage III and IV patients. Similar result was found in the examination of lymphocyte responsiveness with relation to tumor size, where lymphocytes from patients with a large tumor of more than 5 cm in diameter were less responsive when cultured with autologous serum. From these results, it is reasonable to conclude that in patients with breast cancer, the apparent reduction of lymphocyte reactivity may be due to serum inhibitor that may adhere to the lymphocytes, rather than a primary lymphocyte impairment.", "contents": "Lymphocyte responsiveness to phytohemagglutinin and its modification by serum inhibitor in breast cancer patients. Lymphocyte responsiveness in stimulation with phytohemagglutinin (PHA) and the inhibitory effect of autologous serum on lymphocyte responsiveness were investigated in 55 patients with breast cancer. Lymphocytes from patients, even in the late stage of the disease, as well as from normal healthy control, showed an ability to respond to the stimulation with PHA in the presence of pooled normal serum, while lymphocyte responsiveness in the presence of autologous serum was markedly reduced in stage III and IV patients. Similar result was found in the examination of lymphocyte responsiveness with relation to tumor size, where lymphocytes from patients with a large tumor of more than 5 cm in diameter were less responsive when cultured with autologous serum. From these results, it is reasonable to conclude that in patients with breast cancer, the apparent reduction of lymphocyte reactivity may be due to serum inhibitor that may adhere to the lymphocytes, rather than a primary lymphocyte impairment."}
{"id": "PMID:467889", "title": "Potentiation of anticancer effect of carboquone in vivo by glucose pretreatment.", "content": "Anticancer effect of carboquone was notably potentiated in tumor-bearing mice when they were pretreated with 5% glucose solution in the system of blood flow-interrupting hyperthermic chemotherapy. It was further found by quantitative analysis that the potentiation was much greater than increase in the general toxicity of the drug by administration of glucose. Mitomycin-C, on the other hand, revealed no such marked effect though it is considerably similar to carboquone in chemical structure.", "contents": "Potentiation of anticancer effect of carboquone in vivo by glucose pretreatment. Anticancer effect of carboquone was notably potentiated in tumor-bearing mice when they were pretreated with 5% glucose solution in the system of blood flow-interrupting hyperthermic chemotherapy. It was further found by quantitative analysis that the potentiation was much greater than increase in the general toxicity of the drug by administration of glucose. Mitomycin-C, on the other hand, revealed no such marked effect though it is considerably similar to carboquone in chemical structure."}
{"id": "PMID:467891", "title": "Suppression of naturally occurring anti-tissue antibodies during growth and after removal of tumours.", "content": "The sera of normal rats were found to be active in the complement fixation test against isogeneic tumour homogenates. This activity was shown to be heat labile and to sediment with IgM globulins on zone ultracentrifugation in sucrose density gradients. Sera from rats bearing progressively growing tumours and sera from rats, which had such tumours excised, were found to be less active than normal rat sera in the complement fixation test against isogeneic tumour and liver homogenates. It was considered that this suppression of naturally occurring anti-tissue antibodies may be a specific process related to the host immune response to the tumour.", "contents": "Suppression of naturally occurring anti-tissue antibodies during growth and after removal of tumours. The sera of normal rats were found to be active in the complement fixation test against isogeneic tumour homogenates. This activity was shown to be heat labile and to sediment with IgM globulins on zone ultracentrifugation in sucrose density gradients. Sera from rats bearing progressively growing tumours and sera from rats, which had such tumours excised, were found to be less active than normal rat sera in the complement fixation test against isogeneic tumour and liver homogenates. It was considered that this suppression of naturally occurring anti-tissue antibodies may be a specific process related to the host immune response to the tumour."}
{"id": "PMID:467892", "title": "Mutagenicity of quercetin and kaempferol on cultured mammalian cells.", "content": "Mutagenic activity of quercetin and kaempferol on V79 Chinese hamster cells was investigated. Quercetin was proved to have mutagenicity not only by metabolic activation system using rat liver microsomes, but also by direct application method. Kaempferol was mutagenic by metabolic activation system, but its activity by direct method seemed very low. These data suggest that the tested flavonoids seem to be metabolized for exerting their mutagenic activity and V79 cells themselves have an enzymic activation system which metabolizes at a very slow rate but in a cumulative way.", "contents": "Mutagenicity of quercetin and kaempferol on cultured mammalian cells. Mutagenic activity of quercetin and kaempferol on V79 Chinese hamster cells was investigated. Quercetin was proved to have mutagenicity not only by metabolic activation system using rat liver microsomes, but also by direct application method. Kaempferol was mutagenic by metabolic activation system, but its activity by direct method seemed very low. These data suggest that the tested flavonoids seem to be metabolized for exerting their mutagenic activity and V79 cells themselves have an enzymic activation system which metabolizes at a very slow rate but in a cumulative way."}
{"id": "PMID:467893", "title": "Behavior pattern of rat ascites tumor cells arrested in liver sinusoids: an electron microscopic study.", "content": "The behavior pattern of tumor cells in the development of hematogenous liver metastasis by Yoshida sarcoma and two strains of rat ascites hepatoma, AH7974F and AH13, in Donryu strain rats was investigated by electron microscopy. Tumor cells of every strain in liver sinusoids stretched their cytoplasmic protrusions toward the vascular wall and were actually in contact with the endothelial cells with their tips to form various types of junction-like structures. The most distinct one was characterized by parallel and straight arrangement of the opposing cell membranes across a gap of about 100 A. The endothelial discontinuity was easily produced by the projection of cytoplasmic processes and the compression of tumor cells. Through the endothelial defect, tumor cells of Yoshida sarcoma and AH13 migrated locomotively into the dilated spaces of Disse or directly toward the hepatic cells, while AH7974F cells were frequently covered by elongated endothelial cytoplasm. Destruction of the hepatic cells by AH7974F and AH13 cells was found to begin with the insertion of their cytoplasmic processes into the coated vesicles in the hepatic cells. From these investigations, the particular interaction between tumor and normal cells seems to play an important role in the development of liver metastasis by these tumors.", "contents": "Behavior pattern of rat ascites tumor cells arrested in liver sinusoids: an electron microscopic study. The behavior pattern of tumor cells in the development of hematogenous liver metastasis by Yoshida sarcoma and two strains of rat ascites hepatoma, AH7974F and AH13, in Donryu strain rats was investigated by electron microscopy. Tumor cells of every strain in liver sinusoids stretched their cytoplasmic protrusions toward the vascular wall and were actually in contact with the endothelial cells with their tips to form various types of junction-like structures. The most distinct one was characterized by parallel and straight arrangement of the opposing cell membranes across a gap of about 100 A. The endothelial discontinuity was easily produced by the projection of cytoplasmic processes and the compression of tumor cells. Through the endothelial defect, tumor cells of Yoshida sarcoma and AH13 migrated locomotively into the dilated spaces of Disse or directly toward the hepatic cells, while AH7974F cells were frequently covered by elongated endothelial cytoplasm. Destruction of the hepatic cells by AH7974F and AH13 cells was found to begin with the insertion of their cytoplasmic processes into the coated vesicles in the hepatic cells. From these investigations, the particular interaction between tumor and normal cells seems to play an important role in the development of liver metastasis by these tumors."}
{"id": "PMID:467894", "title": "A molecular orbital study on the chemical reactivity and biological activity of polycyclic aromatic hydrocarbon diol-epoxides in connection with bay region theory.", "content": "Electronic structures of naphthalene-, anthracene-, phenanthrene-, and benzo-[a]pyrene-diol-epoxides were calculated by using CNDO/2 method together with the energy minimization method. By using these methods, the most stable geometry of molecules was automatically obtained. By comparing the total energy of phenanthrene-diol-epoxide with that of anthracene-diol-epoxide, the role of the bay region was studied in connection with the chemical reactivity. It is concluded that the bay region plays an important role when the diol-epoxide reacts with the component of the biological system via SNl mechanism. Moreover, benzo[a]pyrene-diol-epoxide was shown to be very reactive because fo the presence of the bay region as well as the large size of the molecule. When reaction proceeds via SN2 mechanism, the bay region is found to have little effect on the chemical reactivity of the molecules.", "contents": "A molecular orbital study on the chemical reactivity and biological activity of polycyclic aromatic hydrocarbon diol-epoxides in connection with bay region theory. Electronic structures of naphthalene-, anthracene-, phenanthrene-, and benzo-[a]pyrene-diol-epoxides were calculated by using CNDO/2 method together with the energy minimization method. By using these methods, the most stable geometry of molecules was automatically obtained. By comparing the total energy of phenanthrene-diol-epoxide with that of anthracene-diol-epoxide, the role of the bay region was studied in connection with the chemical reactivity. It is concluded that the bay region plays an important role when the diol-epoxide reacts with the component of the biological system via SNl mechanism. Moreover, benzo[a]pyrene-diol-epoxide was shown to be very reactive because fo the presence of the bay region as well as the large size of the molecule. When reaction proceeds via SN2 mechanism, the bay region is found to have little effect on the chemical reactivity of the molecules."}
{"id": "PMID:467895", "title": "Tumor formation of rat ascites hepatoma cells in the traumatized brain.", "content": "The effect of brain trauma on the formation of metastatic growth was examined by iv injection of 6 strains of rat ascites hepatoma. Three of the tumors (AH7974F, AH66F, and AH13) were in the single-cell state and three (AH272, AH7974, and AH601) contained cell aggregates. None of the tumors showed any tumor growth in the intact brain after injection into the tail vein. However, after traumatization of the brain tissue it was possible to observe tumor formation in the lesion, mainly by injection of the single-cell type tumors. The brain tissue was most susceptive to the tumor cells 10 min and 7 days after trauma. On the other hand, after the injection into the carotid artery of traumatized animals, the tumor formation in the brain was found in both tumor groups.", "contents": "Tumor formation of rat ascites hepatoma cells in the traumatized brain. The effect of brain trauma on the formation of metastatic growth was examined by iv injection of 6 strains of rat ascites hepatoma. Three of the tumors (AH7974F, AH66F, and AH13) were in the single-cell state and three (AH272, AH7974, and AH601) contained cell aggregates. None of the tumors showed any tumor growth in the intact brain after injection into the tail vein. However, after traumatization of the brain tissue it was possible to observe tumor formation in the lesion, mainly by injection of the single-cell type tumors. The brain tissue was most susceptive to the tumor cells 10 min and 7 days after trauma. On the other hand, after the injection into the carotid artery of traumatized animals, the tumor formation in the brain was found in both tumor groups."}
{"id": "PMID:467896", "title": "Production of proliferation lesions in gastric mucosa of albino mice by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "It was possible to produce gastric adenocarcinoma in five of 69, healthy, young albino mice of both sexes given N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in their drinking water at a concentration of 100 microgram/ml. The tumoral lesions were most clearly defined in the group that received MNNG for the longest period of time, that is, 68 weeks. On the other hand, in the rest of the groups, which received MNNG for fewer weeks, there were lesions found in a total of nine animals which consisted in foci of typical and atypical hyperplasia, erosion of the mucosa, and, in one animal, an adenomatous polyp. Contrary to findings in humans, intestinal metaplasia preceding or accompanying the neoplasia was found to be an inconsistent alteration and a not very frequent one.", "contents": "Production of proliferation lesions in gastric mucosa of albino mice by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine. It was possible to produce gastric adenocarcinoma in five of 69, healthy, young albino mice of both sexes given N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in their drinking water at a concentration of 100 microgram/ml. The tumoral lesions were most clearly defined in the group that received MNNG for the longest period of time, that is, 68 weeks. On the other hand, in the rest of the groups, which received MNNG for fewer weeks, there were lesions found in a total of nine animals which consisted in foci of typical and atypical hyperplasia, erosion of the mucosa, and, in one animal, an adenomatous polyp. Contrary to findings in humans, intestinal metaplasia preceding or accompanying the neoplasia was found to be an inconsistent alteration and a not very frequent one."}
{"id": "PMID:467897", "title": "Effect of uracil on metabolism of 5-fluorouracil in vitro.", "content": "The effect of uracil on the metabolism of 5-fluorouracil (5-FU) in vitro was studied. 5-FU was mainly phosphorylated in intact Yoshida sarcoma cells, whereas it was mainly degraded in liver slices. Uracil inhibited degradation of 5-FU much more than its phosphorylation; incubation of 2,500 microM of uracil with 2.5 microM of 5-FU (molar ratio, 1,000:1) inhibited the degradation of 5-FU by 70%, but did not affect its phosphorylation. With homogenates of Yoshida sarcoma or liver uracil inhibited degradation of 5-FU greatly, phosphorylation of 5-FU by alpha-D-ribose 1-phosphate (RiblP) and ATP to some extent, and phosphorylation by 5-phospho-alpha-D-ribosyl diphosphate (PPRibP) very little. The activities of the enzymes involved in the metabolism of 5-FU in various tissues were also determined. Degradation of 5-FU was much faster in liver than in other tissues and was very slow in tumor tissue. Phosphorylation of 5-FU with RiblP and ATP was rapid in Yoshida sarcoma and bone marrow. Phosphoribosyltransferase activity was high in Yoshida sarcoma and thymus, but low in bone marrow.", "contents": "Effect of uracil on metabolism of 5-fluorouracil in vitro. The effect of uracil on the metabolism of 5-fluorouracil (5-FU) in vitro was studied. 5-FU was mainly phosphorylated in intact Yoshida sarcoma cells, whereas it was mainly degraded in liver slices. Uracil inhibited degradation of 5-FU much more than its phosphorylation; incubation of 2,500 microM of uracil with 2.5 microM of 5-FU (molar ratio, 1,000:1) inhibited the degradation of 5-FU by 70%, but did not affect its phosphorylation. With homogenates of Yoshida sarcoma or liver uracil inhibited degradation of 5-FU greatly, phosphorylation of 5-FU by alpha-D-ribose 1-phosphate (RiblP) and ATP to some extent, and phosphorylation by 5-phospho-alpha-D-ribosyl diphosphate (PPRibP) very little. The activities of the enzymes involved in the metabolism of 5-FU in various tissues were also determined. Degradation of 5-FU was much faster in liver than in other tissues and was very slow in tumor tissue. Phosphorylation of 5-FU with RiblP and ATP was rapid in Yoshida sarcoma and bone marrow. Phosphoribosyltransferase activity was high in Yoshida sarcoma and thymus, but low in bone marrow."}
{"id": "PMID:467898", "title": "Transplantable ileal adenocarcinomas of ACI rats.", "content": "Transplantation of 9 kinds of bracken-induced ileal adenocarcinomas subcutaneously in ACI rats established two transplantable strains of adenocarcinoma (73-357, 77-238). Ascitic conversion succeeded in the strain 73-357. Biological and morphological, particularly electron microscopic, characteristics of these strains are reported.", "contents": "Transplantable ileal adenocarcinomas of ACI rats. Transplantation of 9 kinds of bracken-induced ileal adenocarcinomas subcutaneously in ACI rats established two transplantable strains of adenocarcinoma (73-357, 77-238). Ascitic conversion succeeded in the strain 73-357. Biological and morphological, particularly electron microscopic, characteristics of these strains are reported."}
{"id": "PMID:467899", "title": "Tumor development in lung of ddY mice following transplacental exposure to 1-ethyl-1-nitrosourea.", "content": "Transplacental induction of lung tumor by 1-ethyl-1-nitrosourea (ENU) was studied in pregnant ddY mice which were given a single intraperitoneal injection of 58.5 mg/kg of ENU in water between day 13 and 19 of gestation. Within 4 approximately 6 weeks after birth, pulmonary tumor nodules were found in all offsprings exposed to ENU, and they were histopathologically adenoma. Number of tumor nodules could be counted under the stereomicroscope from approximately day 40 after birth. The size of tumor increased with the lapse of time but the number of tumor nodules did not increase markedly. Weekly injections of urethan or ENU into mice pretreated with ENU in their fetal age enhanced the number of pulmonary adenoma. The development of other tumor was not seen except a few cases of lymphoma. Tumor development in the lung by injection of ENU in ddY mice during gestation is reproducible, relatively simple, and rapid. Therefore, it is considered that this may be a useful method for screening of antitumor agent.", "contents": "Tumor development in lung of ddY mice following transplacental exposure to 1-ethyl-1-nitrosourea. Transplacental induction of lung tumor by 1-ethyl-1-nitrosourea (ENU) was studied in pregnant ddY mice which were given a single intraperitoneal injection of 58.5 mg/kg of ENU in water between day 13 and 19 of gestation. Within 4 approximately 6 weeks after birth, pulmonary tumor nodules were found in all offsprings exposed to ENU, and they were histopathologically adenoma. Number of tumor nodules could be counted under the stereomicroscope from approximately day 40 after birth. The size of tumor increased with the lapse of time but the number of tumor nodules did not increase markedly. Weekly injections of urethan or ENU into mice pretreated with ENU in their fetal age enhanced the number of pulmonary adenoma. The development of other tumor was not seen except a few cases of lymphoma. Tumor development in the lung by injection of ENU in ddY mice during gestation is reproducible, relatively simple, and rapid. Therefore, it is considered that this may be a useful method for screening of antitumor agent."}
{"id": "PMID:467918", "title": "Nucleation time: a key factor in the pathogenesis of cholesterol gallstone disease.", "content": "In earlier studies, we concluded that biliary cholesterol supersaturation may be a necessary but not sufficient cause for gallstone formation. In the present studies, we calculated cholesterol saturation indices (CSI) for 120 bile specimens of cholesterol gallstone patients and controls, using the solubility boundaries of Carey and Small (J Clin Invest 61:998-1026, 1978) for artificial biles. The mean CSI + SD was 1.42 +/- 0.68 for controls and 1.80 +/- 1.02 for cholesterol gallstone patients. Of the control bile samples, 68% were supersaturated. Since the two groups could not be sharply distinguished by saturation index, we studied another property of bile samples: nucleation time for cholesterol crystal formation. The mean nucleation time for 16 control bile samples was 15 days, and a strong correlation (r = 0.84) was found between CSI and nucleation time. Twelve bile samples of cholesterol gallstone patients had a mean nucleation time of 3 days and no correlation between CSI and nucleation time. A discriminant analysis of the data, which took into account both the CSI and the nucleation time for each sample, allowed a sharp distinction between bile samples of cholesterol gallstone patients and controls. This distinction could not have been drawn from studies with artificial biles, since these differ only in their CSI.", "contents": "Nucleation time: a key factor in the pathogenesis of cholesterol gallstone disease. In earlier studies, we concluded that biliary cholesterol supersaturation may be a necessary but not sufficient cause for gallstone formation. In the present studies, we calculated cholesterol saturation indices (CSI) for 120 bile specimens of cholesterol gallstone patients and controls, using the solubility boundaries of Carey and Small (J Clin Invest 61:998-1026, 1978) for artificial biles. The mean CSI + SD was 1.42 +/- 0.68 for controls and 1.80 +/- 1.02 for cholesterol gallstone patients. Of the control bile samples, 68% were supersaturated. Since the two groups could not be sharply distinguished by saturation index, we studied another property of bile samples: nucleation time for cholesterol crystal formation. The mean nucleation time for 16 control bile samples was 15 days, and a strong correlation (r = 0.84) was found between CSI and nucleation time. Twelve bile samples of cholesterol gallstone patients had a mean nucleation time of 3 days and no correlation between CSI and nucleation time. A discriminant analysis of the data, which took into account both the CSI and the nucleation time for each sample, allowed a sharp distinction between bile samples of cholesterol gallstone patients and controls. This distinction could not have been drawn from studies with artificial biles, since these differ only in their CSI."}
{"id": "PMID:467919", "title": "Successful medical treatment of pyogenic liver abscess.", "content": "Between 1976 and 1978, 6 patients with proven or probable pyogenic liver abscess were successfully treated at Los Angeles County-University of Southern California Medical Center without surgical drainage. Although surgical therapy is necessary in patients with coexisting intraabdominal pathology, antibiotic therapy without surgical intervention is an option to be considered in patients with pyogenic liver abscess and no evidence of other intraabdominal disease.", "contents": "Successful medical treatment of pyogenic liver abscess. Between 1976 and 1978, 6 patients with proven or probable pyogenic liver abscess were successfully treated at Los Angeles County-University of Southern California Medical Center without surgical drainage. Although surgical therapy is necessary in patients with coexisting intraabdominal pathology, antibiotic therapy without surgical intervention is an option to be considered in patients with pyogenic liver abscess and no evidence of other intraabdominal disease."}
{"id": "PMID:467921", "title": "Control of upper gastrointestinal hemorrhage by endoscopic spraying of clotting factors.", "content": "A new technique for the endoscopic control of upper gastrointestinal hemorrhage has been developed. It consists of a spraying device which delivers atomized clotting factors (thrombin and fibrinogen) directly to a focal bleeding site. In vivo studies in a standardized dog model of acute gastric ulcer bleeding, demonstrated a significant reduction in bleeding time by spraying thrombin and cryoprecipitate (mean +/- SE bleeding time = 223 +/- 14 sec) as compared to a saline spray (mean 705 +/- 60 sec) or to the untreated controls (mean 498 +/- 43 sec), P less than 0.001. Similar results were obtained in the heparinized dog model. The mean bleeding time was 226 +/- 11 sec in the sprayed animals, while the control ulcers bled continuously. The four lumen spray tube fits the biopsy lumen of commercial gastroscopes. The feasibility of the technique for treatment of oozing venous bleeding sites in the upper gastrointestinal tract has been successfully tried in a clinical setting. The authors feel that the technique in its present form is ready for clinical testing in a controlled manner.", "contents": "Control of upper gastrointestinal hemorrhage by endoscopic spraying of clotting factors. A new technique for the endoscopic control of upper gastrointestinal hemorrhage has been developed. It consists of a spraying device which delivers atomized clotting factors (thrombin and fibrinogen) directly to a focal bleeding site. In vivo studies in a standardized dog model of acute gastric ulcer bleeding, demonstrated a significant reduction in bleeding time by spraying thrombin and cryoprecipitate (mean +/- SE bleeding time = 223 +/- 14 sec) as compared to a saline spray (mean 705 +/- 60 sec) or to the untreated controls (mean 498 +/- 43 sec), P less than 0.001. Similar results were obtained in the heparinized dog model. The mean bleeding time was 226 +/- 11 sec in the sprayed animals, while the control ulcers bled continuously. The four lumen spray tube fits the biopsy lumen of commercial gastroscopes. The feasibility of the technique for treatment of oozing venous bleeding sites in the upper gastrointestinal tract has been successfully tried in a clinical setting. The authors feel that the technique in its present form is ready for clinical testing in a controlled manner."}
{"id": "PMID:467920", "title": "Cholesterol gallstone formation and prevention by chenodeoxycholic and ursodeoxycholic acids. A new hamster model.", "content": "Prior animal models of cholesterol gallstone formation have been criticized for their dissimilarity to the conditions of humans with gallstones. We present a new hamster model of cholesterol cholelithiasis that more closely approximates the human situation. Sixty female Golden Syrian hamsters (average weight 83.2 +/- 3.4 g) were allocated to six groups of 10 animals each. Groups were fed standard diet (containing 0.8 gm cholesterol/g of food) or increased cholesterol diet (containing 2.4 mg cholesterol/g of food), with or without ethinyl estradiol, 15 micrograms/kg/d. Two groups receiving both increased cholesterol and ethinyl estradiol also received either chenodeoxycholic acid or ursodeoxycholic acid, 20 mg/kg/d. The animsl were sacrificed at 12 wk. Cholesterol gallstones (78.3 +/- 5.0% cholesterol by weight) formed in 30% of the animals fed ethinyl estradiol, 50% of those fed increased cholesterol, and 90% of those fed the combination of both. Bile was saturated in all three groups, with the saturation index of the combination group (2.08 +/- 0.17) being the highest. In both groups receiving bile acid therapy, no gallstones were found, and the bile remained unsaturated. For the bile acid-fed groups, both hepatic HMG-CoAR and hepatic cholesterol 7 alpha-hydroxylase activities were reduced (P less than 0.01) when compared to the group fed standard diet and to the grou fed the combination. Thus, a new animal model of cholesterol gallstone formation has been developed in which chenodeoxycholic acid and ursodeoxycholic acid therapy prevented gallstone formation through mechanisms similar to those reported in cholesterol gallstone patients.", "contents": "Cholesterol gallstone formation and prevention by chenodeoxycholic and ursodeoxycholic acids. A new hamster model. Prior animal models of cholesterol gallstone formation have been criticized for their dissimilarity to the conditions of humans with gallstones. We present a new hamster model of cholesterol cholelithiasis that more closely approximates the human situation. Sixty female Golden Syrian hamsters (average weight 83.2 +/- 3.4 g) were allocated to six groups of 10 animals each. Groups were fed standard diet (containing 0.8 gm cholesterol/g of food) or increased cholesterol diet (containing 2.4 mg cholesterol/g of food), with or without ethinyl estradiol, 15 micrograms/kg/d. Two groups receiving both increased cholesterol and ethinyl estradiol also received either chenodeoxycholic acid or ursodeoxycholic acid, 20 mg/kg/d. The animsl were sacrificed at 12 wk. Cholesterol gallstones (78.3 +/- 5.0% cholesterol by weight) formed in 30% of the animals fed ethinyl estradiol, 50% of those fed increased cholesterol, and 90% of those fed the combination of both. Bile was saturated in all three groups, with the saturation index of the combination group (2.08 +/- 0.17) being the highest. In both groups receiving bile acid therapy, no gallstones were found, and the bile remained unsaturated. For the bile acid-fed groups, both hepatic HMG-CoAR and hepatic cholesterol 7 alpha-hydroxylase activities were reduced (P less than 0.01) when compared to the group fed standard diet and to the grou fed the combination. Thus, a new animal model of cholesterol gallstone formation has been developed in which chenodeoxycholic acid and ursodeoxycholic acid therapy prevented gallstone formation through mechanisms similar to those reported in cholesterol gallstone patients."}
{"id": "PMID:467922", "title": "Progressive systemic sclerosis of the gastrointestinal tract and hereditary hollow visceral myopathy: two distinguishable disorders of intestinal smooth muscle.", "content": "The purpose of this study was to determine whether progressive systemic sclerosis (PSS) of the gastrointestinal tract and hereditary hollow visceral myopathy are two distinguishable disorders of intestinal smooth muscle. We coded and blindly reviewed 50 specimens of tissue from the small intestine of 15 normal controls, 4 patients with visceral myopathy, 5 patients with PSS and intestinal pseudoobstruction, and 5 patients with PSS but no pseudoobstruction. We determined that there is a fundamental difference between the pathology of these two disorders. In visceral myopathy, the smooth muscle is characterized by vacuolar degeneration and fibrosis. In PSS, smooth muscle fibrosis is present but vacuolar degeneration is absent. Although smooth muscle cells are decreased in number in both disorders, those that are present in PSS are morphologically normal by light microscopy, whereas most of those present in visceral myopathy are degenerated. Visceral myopathy and gastrointestinal PSS are two distinct disorders of smooth muscle which are easily distinguished by conventional light microscopy. Their dissimilar appearances and the familial nature of visceral myopathy suggest that they result from quite different causes.", "contents": "Progressive systemic sclerosis of the gastrointestinal tract and hereditary hollow visceral myopathy: two distinguishable disorders of intestinal smooth muscle. The purpose of this study was to determine whether progressive systemic sclerosis (PSS) of the gastrointestinal tract and hereditary hollow visceral myopathy are two distinguishable disorders of intestinal smooth muscle. We coded and blindly reviewed 50 specimens of tissue from the small intestine of 15 normal controls, 4 patients with visceral myopathy, 5 patients with PSS and intestinal pseudoobstruction, and 5 patients with PSS but no pseudoobstruction. We determined that there is a fundamental difference between the pathology of these two disorders. In visceral myopathy, the smooth muscle is characterized by vacuolar degeneration and fibrosis. In PSS, smooth muscle fibrosis is present but vacuolar degeneration is absent. Although smooth muscle cells are decreased in number in both disorders, those that are present in PSS are morphologically normal by light microscopy, whereas most of those present in visceral myopathy are degenerated. Visceral myopathy and gastrointestinal PSS are two distinct disorders of smooth muscle which are easily distinguished by conventional light microscopy. Their dissimilar appearances and the familial nature of visceral myopathy suggest that they result from quite different causes."}
{"id": "PMID:467924", "title": "Effect of vagotomy in Zollinger-Ellison syndrome.", "content": "We evaluated the effect of vagotomy on gastric acid secretion and the clinical course in 3 patients with Zollinger-Ellison syndrome. Basal acid hypersecretion was reduced by 49, 86, and 96%, and peak acid output in response to pentagastrin was reduced by 36, 39, and 71% in the 3 patients. In one patient, 300 mg cimetidine reduced basal acid secretion from 65 to 20 meq/hr before vagotomy; whereas after vagotomy basal acid secretion was reduced from 36 to 0.6 meq/hr by the same dose of cimetidine. One patient has required no antisecretory therapy for 14 yr, whereas 2 patients have also been treated with cimetidine with excellent results. We conclude that vagotomy facilitates control of acid secretion in Zollinger-Ellison syndrome, and we recommend vagotomy and cimetidine rather than total gastrectomy or cimetidine alone for the management of these patients. This combined surgical and medical approach should also allow discovery and removal of isolated tumors in about 10% of patients.", "contents": "Effect of vagotomy in Zollinger-Ellison syndrome. We evaluated the effect of vagotomy on gastric acid secretion and the clinical course in 3 patients with Zollinger-Ellison syndrome. Basal acid hypersecretion was reduced by 49, 86, and 96%, and peak acid output in response to pentagastrin was reduced by 36, 39, and 71% in the 3 patients. In one patient, 300 mg cimetidine reduced basal acid secretion from 65 to 20 meq/hr before vagotomy; whereas after vagotomy basal acid secretion was reduced from 36 to 0.6 meq/hr by the same dose of cimetidine. One patient has required no antisecretory therapy for 14 yr, whereas 2 patients have also been treated with cimetidine with excellent results. We conclude that vagotomy facilitates control of acid secretion in Zollinger-Ellison syndrome, and we recommend vagotomy and cimetidine rather than total gastrectomy or cimetidine alone for the management of these patients. This combined surgical and medical approach should also allow discovery and removal of isolated tumors in about 10% of patients."}
{"id": "PMID:467926", "title": "Release of somatostatin-like immunoreactivity from the lower gut.", "content": "The present study describes the effect of the instillation of a nutrient-containing hyperosmolar volume load into the lower ileum upon plasma levels of somatostatin-like immunoreactivity. The baseline levels of SLI in the mesenteric vein were significantly higher than those in the inferior vena cava and rose significantly in both veins in response to the nutrient load. SLI levels in the venous effluent of the pancreas and stomach did not rise. Mesenteric vein SLI was similar in molecular size to the SLI in the gastric and pancreatic veins and to synthetic somatostatin. The results suggest that the lower gut may contribute to the basal circulating SLI levels and that it is released from the lower gut in response to a nutrient-containing hyperosmolar volume load.", "contents": "Release of somatostatin-like immunoreactivity from the lower gut. The present study describes the effect of the instillation of a nutrient-containing hyperosmolar volume load into the lower ileum upon plasma levels of somatostatin-like immunoreactivity. The baseline levels of SLI in the mesenteric vein were significantly higher than those in the inferior vena cava and rose significantly in both veins in response to the nutrient load. SLI levels in the venous effluent of the pancreas and stomach did not rise. Mesenteric vein SLI was similar in molecular size to the SLI in the gastric and pancreatic veins and to synthetic somatostatin. The results suggest that the lower gut may contribute to the basal circulating SLI levels and that it is released from the lower gut in response to a nutrient-containing hyperosmolar volume load."}
{"id": "PMID:467928", "title": "Mechanisms of mucus release in exposed canine gastric mucosa.", "content": "Mucus release was studied in the exposed gastric mucosa of anesthetized fasted dogs using scanning and transmission microscopy as well as histochemical and autoradiographic techniques. Under unstimulated conditions, the gastric epithelium was composed of both nonsecreting and mucus-secreting epithelial cells, with the former being predominant. Nonsecreting cells were characterized by an intact apical mucus package of granules and a continuous plasma membrane. The secreting mucus cell population was found in the foveolar (pit region) as well as interfoveolar areas. Three mechanisms of mucus release were observed: (a) exocytosis, (b) apical expulsion, and (c) cell exfoliation. Evidence for exocytosis was found in all mucus cells, especially in the sulfated glycoprotein-rich foveolar cells. Exocytosis involved only a few granules at a time; this mode of secretion is likely slow and continuous. In contrast, apical expulsion resulted in an explosive release of the entire apical mucus package followed by in situ degeneration of the cell itself. This occurred in the oldest cells forming mucosal crests in the interfoveolar area, whose mucus predominantly stains for neutral glycoproteins. Cell exfoliation, in which the entire cell was extruded into the lumen, was rarely observed and may provide, in addition to apical expulsion, a second mechanism to rid the mucosa of senescent epithelial cells. Mucus secretion is a complex function of the gastric epithelium. The mechanism of secretion and the histochemically defined type of mucus secreted are variables which are dependent on the age of the cell, its position on the foveolae, and the microenvironment within the gastric lumen. The mucus-containing surface and pit cells of gastric epithelium have been described morphologically and ultrastructurally in a number of studies. These cells are highly differentiated, forming a layer which is dynamic and responsive to conditions present in the gastric lumen. Mucus cells arise from multipotent progenitor cells which differentiate in the course of migration up the gastric pits and are involved in the complex macromolecular synthesis of glycoproteins. Although mucus release occurs throughout cell life, very little attention has been given to the cellular ultrastructural changes that deal with mucus secretion. As a result, the mucus-containing surface and pit cells have been described primarily in terms of their nonsecreting functional state. Exceptions to this are a few transmission and scanning electron microscopy studies which describe loss of intact mucus granules, cell extrusion, and in situ degeneration. These previous ultrastructural studies describe cellular patterns, in both normal and injured mucosa, which we fell are related to mucus release. Because of a paucity of knowledge concerning the normal mechanisms of mucus secretion, it was the purpose of this study to define the ultrastructural changes which result in and accompany the production of mucus in canine gastric epithelium.", "contents": "Mechanisms of mucus release in exposed canine gastric mucosa. Mucus release was studied in the exposed gastric mucosa of anesthetized fasted dogs using scanning and transmission microscopy as well as histochemical and autoradiographic techniques. Under unstimulated conditions, the gastric epithelium was composed of both nonsecreting and mucus-secreting epithelial cells, with the former being predominant. Nonsecreting cells were characterized by an intact apical mucus package of granules and a continuous plasma membrane. The secreting mucus cell population was found in the foveolar (pit region) as well as interfoveolar areas. Three mechanisms of mucus release were observed: (a) exocytosis, (b) apical expulsion, and (c) cell exfoliation. Evidence for exocytosis was found in all mucus cells, especially in the sulfated glycoprotein-rich foveolar cells. Exocytosis involved only a few granules at a time; this mode of secretion is likely slow and continuous. In contrast, apical expulsion resulted in an explosive release of the entire apical mucus package followed by in situ degeneration of the cell itself. This occurred in the oldest cells forming mucosal crests in the interfoveolar area, whose mucus predominantly stains for neutral glycoproteins. Cell exfoliation, in which the entire cell was extruded into the lumen, was rarely observed and may provide, in addition to apical expulsion, a second mechanism to rid the mucosa of senescent epithelial cells. Mucus secretion is a complex function of the gastric epithelium. The mechanism of secretion and the histochemically defined type of mucus secreted are variables which are dependent on the age of the cell, its position on the foveolae, and the microenvironment within the gastric lumen. The mucus-containing surface and pit cells of gastric epithelium have been described morphologically and ultrastructurally in a number of studies. These cells are highly differentiated, forming a layer which is dynamic and responsive to conditions present in the gastric lumen. Mucus cells arise from multipotent progenitor cells which differentiate in the course of migration up the gastric pits and are involved in the complex macromolecular synthesis of glycoproteins. Although mucus release occurs throughout cell life, very little attention has been given to the cellular ultrastructural changes that deal with mucus secretion. As a result, the mucus-containing surface and pit cells have been described primarily in terms of their nonsecreting functional state. Exceptions to this are a few transmission and scanning electron microscopy studies which describe loss of intact mucus granules, cell extrusion, and in situ degeneration. These previous ultrastructural studies describe cellular patterns, in both normal and injured mucosa, which we fell are related to mucus release. Because of a paucity of knowledge concerning the normal mechanisms of mucus secretion, it was the purpose of this study to define the ultrastructural changes which result in and accompany the production of mucus in canine gastric epithelium."}
{"id": "PMID:467930", "title": "A case of familial visceral myopathy with atrophy and fibrosis of the longitudinal muscle layer of the entire small bowel.", "content": "The case of a 21-yr-old man, who died in cachexia after 2 yr of intermittent abdominal pain, bouts of diarrhea, and anorexia, is reported. Laboratory tests performed shortly before death disclosed signs of malabsorption. Radiologic examination of the gastrointestinal tract showed a coarse mucosal relief in the upper jejunum and a tubular aspect in the rest of the small bowel. There was no dilatation of the loops. Autopsy revealed severe to complete atrophy with fibrosis of the outer muscle layer of the entire small bowel, extending from the pylorus to the ileocecal valve. The only other lesion discovered was a moderate portoportal fibrosis of the liver. The patient's brother had died a few months earlier after 2.5 yr of similar symptoms. An upper gastrointestinal series had shown dilatation of the stomach with fluid retention and a tubular aspect of the small bowel with generalized widening of the loops. No autopsy was performed. There was a high degree of consanguinity on the mother's side. Family history revealed no other evidence of possible genetic factors in the disease.", "contents": "A case of familial visceral myopathy with atrophy and fibrosis of the longitudinal muscle layer of the entire small bowel. The case of a 21-yr-old man, who died in cachexia after 2 yr of intermittent abdominal pain, bouts of diarrhea, and anorexia, is reported. Laboratory tests performed shortly before death disclosed signs of malabsorption. Radiologic examination of the gastrointestinal tract showed a coarse mucosal relief in the upper jejunum and a tubular aspect in the rest of the small bowel. There was no dilatation of the loops. Autopsy revealed severe to complete atrophy with fibrosis of the outer muscle layer of the entire small bowel, extending from the pylorus to the ileocecal valve. The only other lesion discovered was a moderate portoportal fibrosis of the liver. The patient's brother had died a few months earlier after 2.5 yr of similar symptoms. An upper gastrointestinal series had shown dilatation of the stomach with fluid retention and a tubular aspect of the small bowel with generalized widening of the loops. No autopsy was performed. There was a high degree of consanguinity on the mother's side. Family history revealed no other evidence of possible genetic factors in the disease."}
{"id": "PMID:467931", "title": "Fecal incontinence in chronic diarrhea. Report of a case with improvement after training with rectally infused saline.", "content": "Patients with chronic diarrhea and fecal in continence are unable to retain as much rectally infused saline as patients without incontinence. We explored the effect of training such a patient to retain rectally infused saline. The patient was a 31-yr-old female with chronic diarrhea of obscure etiology who had daily episodes of fecal incontinence which markedly restricted her lifestyle. Training was accomplished by urging the patient to retain as much of a 25-min rectal infusion of 1500 ml saline as possible. After 10 training sessions, the patient increased her ability to hold rectally infused saline almost sevenfold. This increase was well maintained over 10 wk. In spite of continued diarrhea, the patient's incontinence did not recur after the first week of training, and she was able to resume a normal life. Anal sphincter pressure and a test of continence for a solid sphere did not change during or after training. This simple training technique has potential as a treatment for disabling fecal incontinence in patients with chronic diarrhea.", "contents": "Fecal incontinence in chronic diarrhea. Report of a case with improvement after training with rectally infused saline. Patients with chronic diarrhea and fecal in continence are unable to retain as much rectally infused saline as patients without incontinence. We explored the effect of training such a patient to retain rectally infused saline. The patient was a 31-yr-old female with chronic diarrhea of obscure etiology who had daily episodes of fecal incontinence which markedly restricted her lifestyle. Training was accomplished by urging the patient to retain as much of a 25-min rectal infusion of 1500 ml saline as possible. After 10 training sessions, the patient increased her ability to hold rectally infused saline almost sevenfold. This increase was well maintained over 10 wk. In spite of continued diarrhea, the patient's incontinence did not recur after the first week of training, and she was able to resume a normal life. Anal sphincter pressure and a test of continence for a solid sphere did not change during or after training. This simple training technique has potential as a treatment for disabling fecal incontinence in patients with chronic diarrhea."}
{"id": "PMID:467932", "title": "Primary shunt hyperbilirubinemia with secondary iron overload: a case report.", "content": "A patient with familial primary shunt hyperbilirubinemia is described. Splenomegaly, arthritis, and iron overload were striking features of the illness.", "contents": "Primary shunt hyperbilirubinemia with secondary iron overload: a case report. A patient with familial primary shunt hyperbilirubinemia is described. Splenomegaly, arthritis, and iron overload were striking features of the illness."}
{"id": "PMID:467933", "title": "Acute eosinophilic cholecystitis in association with hepatic echinococcosis.", "content": "A patient with acute eosinophilic cholecystitis is presented. This was in association with hepatic hydatid disease and was the presenting feature of that disease.", "contents": "Acute eosinophilic cholecystitis in association with hepatic echinococcosis. A patient with acute eosinophilic cholecystitis is presented. This was in association with hepatic hydatid disease and was the presenting feature of that disease."}
{"id": "PMID:467941", "title": "Rederived values of the eight coefficients of the Crohn's Disease Activity Index (CDAI).", "content": "A previous paper reported derivation of the Crohn's Disease Activity Index (CDAI) from data on 112 patients. We have now rederived the values of the eight coefficients of the CDAI using data from 1058 visits of patients enrolled in the National Cooperative Crohn's Disease Study and the Trial of Adjunctive Sulfasalazine in Crohn's Disease. The rederived coefficients are similar to the original ones. New and original index values calculated on the same data from patient visits correlated very highly. Because there is so little difference between the original and the rederived versions, continued use of the original version is suggested in order to maintain comparability of new studies employing this tool with those previously accomplished.", "contents": "Rederived values of the eight coefficients of the Crohn's Disease Activity Index (CDAI). A previous paper reported derivation of the Crohn's Disease Activity Index (CDAI) from data on 112 patients. We have now rederived the values of the eight coefficients of the CDAI using data from 1058 visits of patients enrolled in the National Cooperative Crohn's Disease Study and the Trial of Adjunctive Sulfasalazine in Crohn's Disease. The rederived coefficients are similar to the original ones. New and original index values calculated on the same data from patient visits correlated very highly. Because there is so little difference between the original and the rederived versions, continued use of the original version is suggested in order to maintain comparability of new studies employing this tool with those previously accomplished."}
{"id": "PMID:467943", "title": "National Cooperative Crohn's Disease Study: extraintestinal manifestations and perianal complications.", "content": "Among 569 patients with Crohn's disease, 24% had a history of at least one extraintestinal manifestation and 36% had a history of perianal disease before randomization. Multiple extraintestinal manifestations occurred in the same patient more frequently than would be expected by chance. Seventy-six percent of patients with ileocolitis had perianal disease, extraintestinal manifestations, or both. This was significantly greater than the 58% incidence in patients with disease confined to the small bowel. Perianal complications alone were significantly more common in patients with colitis or ileocolitis than in those with disease of only the small bowel. This was true also of internal fistulization. There was a significant positive association between perianal disease and the presence of extraintestinal features. Perianal abscess appeared to respond to sulfasalazine and anal fissure to prednisone or azathioprine. These results require confirmation in larger series of patients.", "contents": "National Cooperative Crohn's Disease Study: extraintestinal manifestations and perianal complications. Among 569 patients with Crohn's disease, 24% had a history of at least one extraintestinal manifestation and 36% had a history of perianal disease before randomization. Multiple extraintestinal manifestations occurred in the same patient more frequently than would be expected by chance. Seventy-six percent of patients with ileocolitis had perianal disease, extraintestinal manifestations, or both. This was significantly greater than the 58% incidence in patients with disease confined to the small bowel. Perianal complications alone were significantly more common in patients with colitis or ileocolitis than in those with disease of only the small bowel. This was true also of internal fistulization. There was a significant positive association between perianal disease and the presence of extraintestinal features. Perianal abscess appeared to respond to sulfasalazine and anal fissure to prednisone or azathioprine. These results require confirmation in larger series of patients."}
{"id": "PMID:467944", "title": "Clinical usefulness of rectal biopsy in Crohn's disease.", "content": "Initial rectal biopsies from 349 patients judged on clinical, radiologic, or pathologic grounds to have Crohn's disease were studied, and findings were compared with a variety of clinical varibles. Biopsies from only 54 patients (15% of total) contained histologic changes characteristic of Crohn's disease, and all but one of these were previously known to have colonic involvement. Yield of diagnostic findings was directly associated with the presence of sigmoidoscopic or radiologic abnormality in the distal colon or rectum. Minor, nondiagnostic abnormalities were more common. It is concluded that routine single rectal biopsy in unselected cases of Crohn's disease has limited diagnostic value under usual conditions of practice, and that histologic abnormalities are most likely to be found in patients with known colonic, and especially sigmoid and rectal involvement.", "contents": "Clinical usefulness of rectal biopsy in Crohn's disease. Initial rectal biopsies from 349 patients judged on clinical, radiologic, or pathologic grounds to have Crohn's disease were studied, and findings were compared with a variety of clinical varibles. Biopsies from only 54 patients (15% of total) contained histologic changes characteristic of Crohn's disease, and all but one of these were previously known to have colonic involvement. Yield of diagnostic findings was directly associated with the presence of sigmoidoscopic or radiologic abnormality in the distal colon or rectum. Minor, nondiagnostic abnormalities were more common. It is concluded that routine single rectal biopsy in unselected cases of Crohn's disease has limited diagnostic value under usual conditions of practice, and that histologic abnormalities are most likely to be found in patients with known colonic, and especially sigmoid and rectal involvement."}
{"id": "PMID:467945", "title": "[The value of microsurgical techniques in tubal surgery (author's transl)].", "content": "Tubal surgery by help of microsurgical techniques was performed in 86 women from January 1976 until December 1978. 70 patients suffered from tubal sterility due to pelvic inflammatory disease. 16 women asked for reversal of sterilization. As result of 83 operations 17 life births respectively undisturbed intrauterine pregnancies were achieved. Two patients had an abortion. Three more pregnancies could not be added to the success rate, because in these women we found only one blocked tube intraoperatively, while the contralateral one seemed to be intact. Up to now we haven't had an ectopic pregnancy. In 39 patients the operation was done within the last half year. The value of microsurgery in the field of tubal reconstructive surgery is discussed in regard to the literature and to our own experiences and operative results.", "contents": "[The value of microsurgical techniques in tubal surgery (author's transl)]. Tubal surgery by help of microsurgical techniques was performed in 86 women from January 1976 until December 1978. 70 patients suffered from tubal sterility due to pelvic inflammatory disease. 16 women asked for reversal of sterilization. As result of 83 operations 17 life births respectively undisturbed intrauterine pregnancies were achieved. Two patients had an abortion. Three more pregnancies could not be added to the success rate, because in these women we found only one blocked tube intraoperatively, while the contralateral one seemed to be intact. Up to now we haven't had an ectopic pregnancy. In 39 patients the operation was done within the last half year. The value of microsurgery in the field of tubal reconstructive surgery is discussed in regard to the literature and to our own experiences and operative results."}
{"id": "PMID:467946", "title": "[Comparative clinical investigation of oral contraceptives with different doses (author's transl)].", "content": "The investigation of four oral contraceptives with different doses in adolescent girls showed that bleeding disturbances occur more frequently with low-dose preparations at the beginning of treatment. Nevertheless, these disturbances should be considered indicative of lesser central suppression. For this reason adolescent girls -- especially those with still immature cycles -- should be treated chiefly with preparations having only a low inhibitory effect on the central endocrine system in order not to disturb the maturation of the cycle.", "contents": "[Comparative clinical investigation of oral contraceptives with different doses (author's transl)]. The investigation of four oral contraceptives with different doses in adolescent girls showed that bleeding disturbances occur more frequently with low-dose preparations at the beginning of treatment. Nevertheless, these disturbances should be considered indicative of lesser central suppression. For this reason adolescent girls -- especially those with still immature cycles -- should be treated chiefly with preparations having only a low inhibitory effect on the central endocrine system in order not to disturb the maturation of the cycle."}
{"id": "PMID:467947", "title": "[Comparative biochemical investigations with contraceptives at different dose levels (author's transl)].", "content": "In adolescent girls the administration of hormonal contraception raises special problems because of the still unstable menstrual cycle of this group. The influence of four oral contraceptives with different high doses on the levels of the two gonadotropins, estradiol and progesterone has been studied with the aid of the GnRH-test before, during and after treatment. According to the results obtained low-dose combination preparations should be administered to adolescents with stable cycles as in this group normal physiological conditions reappeared without delay after discontinuation of a one-year treatment. If the cycle pattern is still irregular a low-dose gestagen (\"minipill\") preparation may be chosen. This type of treatment showed the least central inhibition, probably advantageous with regard to the still immature cycle.", "contents": "[Comparative biochemical investigations with contraceptives at different dose levels (author's transl)]. In adolescent girls the administration of hormonal contraception raises special problems because of the still unstable menstrual cycle of this group. The influence of four oral contraceptives with different high doses on the levels of the two gonadotropins, estradiol and progesterone has been studied with the aid of the GnRH-test before, during and after treatment. According to the results obtained low-dose combination preparations should be administered to adolescents with stable cycles as in this group normal physiological conditions reappeared without delay after discontinuation of a one-year treatment. If the cycle pattern is still irregular a low-dose gestagen (\"minipill\") preparation may be chosen. This type of treatment showed the least central inhibition, probably advantageous with regard to the still immature cycle."}
{"id": "PMID:467948", "title": "[Neonatal hypoglycemia (author's transl)].", "content": "The paper describes the symptomatology, pathophysiology, diagnostics and therapy of neonatal hypoglycemia. Early diagnosis is required to prevent brain damage due to hypoglycemia. In addition to conventional enzymatic blood glucose determination a new test strip sensitive for lower concentrations enables the diagnosis exactly.", "contents": "[Neonatal hypoglycemia (author's transl)]. The paper describes the symptomatology, pathophysiology, diagnostics and therapy of neonatal hypoglycemia. Early diagnosis is required to prevent brain damage due to hypoglycemia. In addition to conventional enzymatic blood glucose determination a new test strip sensitive for lower concentrations enables the diagnosis exactly."}
{"id": "PMID:467949", "title": "[Vaginoscopic investigation of the cervical ectopy in the neonate (author's transl)].", "content": "The percentage of the physiologic ectopy of the endocervical epithelium onto the portio vaginalis of the cervix in the neonate was unknown. Vaginoscopy of 124 new born females in our Obstetric unit showed that the ectopy of the cervix is not found in 36% but in almost all cases (90%). Colposcopy and cytology excluded pathology of the portio. The cervical ectopy retracts in the physiologic involution process of the neonate. Vaginoscopy of the newborn was technically quite feasible and in some cases medically necessary.", "contents": "[Vaginoscopic investigation of the cervical ectopy in the neonate (author's transl)]. The percentage of the physiologic ectopy of the endocervical epithelium onto the portio vaginalis of the cervix in the neonate was unknown. Vaginoscopy of 124 new born females in our Obstetric unit showed that the ectopy of the cervix is not found in 36% but in almost all cases (90%). Colposcopy and cytology excluded pathology of the portio. The cervical ectopy retracts in the physiologic involution process of the neonate. Vaginoscopy of the newborn was technically quite feasible and in some cases medically necessary."}
{"id": "PMID:467951", "title": "[Microscopic, micro-chemical and fluorescent optical findings in endometrium with a copper-T intrauterine device in situ (author's transl)].", "content": "The uteri of ten patients who wore a copper-T for several months up to two years were examined immediately following hysterectomy by conventional microscopic techniques, fluorescent optical techniques after Acridin-Orange-Fluorochromization and by histo-chemical techniques for copper. With none of these methods changes in the endometrium could be found which explained the effect of the copper-T to inhibit implantation. Since practically any morphological change from the normal was absent it is suggested that the inhibiting effect on pregnancy of the copper intra-uterine devices is due to biochemical changes of the intra-uterine environment.", "contents": "[Microscopic, micro-chemical and fluorescent optical findings in endometrium with a copper-T intrauterine device in situ (author's transl)]. The uteri of ten patients who wore a copper-T for several months up to two years were examined immediately following hysterectomy by conventional microscopic techniques, fluorescent optical techniques after Acridin-Orange-Fluorochromization and by histo-chemical techniques for copper. With none of these methods changes in the endometrium could be found which explained the effect of the copper-T to inhibit implantation. Since practically any morphological change from the normal was absent it is suggested that the inhibiting effect on pregnancy of the copper intra-uterine devices is due to biochemical changes of the intra-uterine environment."}
{"id": "PMID:467953", "title": "[Prenatal diagnosis of triploidy (69, XXX) (author's transl)].", "content": "By amniocentesis we succeeded in finding a case of triploidy (69,XXX). Because of an extreme fetal dystrophy and missing fetal movements in the 33rd week a severe malformation was suspected. The clinical, ultrasonographical, cytogenetital, hormonal and autopsy findings are reported and discussed.", "contents": "[Prenatal diagnosis of triploidy (69, XXX) (author's transl)]. By amniocentesis we succeeded in finding a case of triploidy (69,XXX). Because of an extreme fetal dystrophy and missing fetal movements in the 33rd week a severe malformation was suspected. The clinical, ultrasonographical, cytogenetital, hormonal and autopsy findings are reported and discussed."}
{"id": "PMID:467955", "title": "[The haemodynamic principle of action of diazoxide (hypertonalum) in the treatment of acute hypertensive crises in late pregnant women (author's transl)].", "content": "In ten hypertensive late pregnant women with passing crises of hypertension cardiovascular analysis with the method of quantitative sphygmometry, the unbloody recording of the blood pressure and the direct electronic measuring of the pulse wave velocity were made before and after the intravenous injection of 300 mg Diazoxid (Hypertonalum). This drug is recommended to the treatment of severe preeclampsia and eclampsia because of lessening the arterial blood pressure and the total peripheral resistance, increasing the heart rate, stroke volume and cardiac output.", "contents": "[The haemodynamic principle of action of diazoxide (hypertonalum) in the treatment of acute hypertensive crises in late pregnant women (author's transl)]. In ten hypertensive late pregnant women with passing crises of hypertension cardiovascular analysis with the method of quantitative sphygmometry, the unbloody recording of the blood pressure and the direct electronic measuring of the pulse wave velocity were made before and after the intravenous injection of 300 mg Diazoxid (Hypertonalum). This drug is recommended to the treatment of severe preeclampsia and eclampsia because of lessening the arterial blood pressure and the total peripheral resistance, increasing the heart rate, stroke volume and cardiac output."}
{"id": "PMID:467956", "title": "[The value of magnesium sulfate in intensive care of severe toxemias of pregnancy (author's transl)].", "content": "A view on the employment of magnesium sulfate in pregnancy is given and the statement of the cardiovascular changes before, during and after the intravenous infusion of 4,0 g magnesium sulfate in ten hypertensive late pregnant women. The drug induces a mild decrease of arterial blood pressure with a distinct increase of stroke volume, cardiac output, heart rate, heart work and the heart's power. The pathologically increased total peripheral resistance is reduced. These hemodynamic effects are very favourable concerning intensive care of severe toxemias of pregnancy.", "contents": "[The value of magnesium sulfate in intensive care of severe toxemias of pregnancy (author's transl)]. A view on the employment of magnesium sulfate in pregnancy is given and the statement of the cardiovascular changes before, during and after the intravenous infusion of 4,0 g magnesium sulfate in ten hypertensive late pregnant women. The drug induces a mild decrease of arterial blood pressure with a distinct increase of stroke volume, cardiac output, heart rate, heart work and the heart's power. The pathologically increased total peripheral resistance is reduced. These hemodynamic effects are very favourable concerning intensive care of severe toxemias of pregnancy."}
{"id": "PMID:467975", "title": "[Adaptive association of inversions in a natural population of the malaria mosquito Anopheles messeae Fall].", "content": "The dependent combination of chromosomal variants by five paracentric inversions (IL1, IL2, IIR1, IIIR1, IIIL1) in Anopheles messeae population from the West Siberia was discovered. It is found that the inversion interactions interactions are the same at both larval and imaginal parts of the population, and they are stable during four years of studying. The inversion of hetero- and homozygotes demonstrates identical properties, that is, phylogenetic secondary sequencies dominate in heterozygotes. Two associative groups of chromosome variants were revealed. The association correlates with geographic distribution and temporal dynamics of inversions and on the whole, it reflects the evolutionary history of inversion polymorphism in A. messeae. It is supposed that the adaptive association of inversions found is the high degree polymorphism formed and functioned on the basis of the groups of inversion chromosome blocks.", "contents": "[Adaptive association of inversions in a natural population of the malaria mosquito Anopheles messeae Fall]. The dependent combination of chromosomal variants by five paracentric inversions (IL1, IL2, IIR1, IIIR1, IIIL1) in Anopheles messeae population from the West Siberia was discovered. It is found that the inversion interactions interactions are the same at both larval and imaginal parts of the population, and they are stable during four years of studying. The inversion of hetero- and homozygotes demonstrates identical properties, that is, phylogenetic secondary sequencies dominate in heterozygotes. Two associative groups of chromosome variants were revealed. The association correlates with geographic distribution and temporal dynamics of inversions and on the whole, it reflects the evolutionary history of inversion polymorphism in A. messeae. It is supposed that the adaptive association of inversions found is the high degree polymorphism formed and functioned on the basis of the groups of inversion chromosome blocks."}
{"id": "PMID:467976", "title": "[Cytogenetic effects induced by neutrons in human peripheral blood lymphocytes in vitro. I. Dose-effect relationship for different types of chromosome aberrations when exposed to neutrons with different energies].", "content": "Human lymphocytes were irradiated in vitro during G0 stage by graded doses of thermal neutrons and of neutrons with mean energy of 0.04; 0.09; 0.35; 0.85 and 14.7 MeV as well as by 60Co gamma-rays. The data were fitted to the linear and linear-quadratic relations. The neutrons of low and intermediate energies showed the linear dependence on the dose, 14.7 MeV neutrons and gamma-rays--a linear-quadratic one, whereas the data obtained with 0.85 MeV neutrons fitted well the both models. Terminal and interstitial deletions produced by both gamma-rays and neutrons showed different dependencies upon the dose. Some qualitative pecularities of aberration spectra were found in the experiments with neutrons as compared with the data on gamma-irradiation: the ratio of exchanges to fragments was greater, and aberrations of chromatid type were produced. The specially designed experiments and calculations showed that the last effect was not connected with induced radioactivity.", "contents": "[Cytogenetic effects induced by neutrons in human peripheral blood lymphocytes in vitro. I. Dose-effect relationship for different types of chromosome aberrations when exposed to neutrons with different energies]. Human lymphocytes were irradiated in vitro during G0 stage by graded doses of thermal neutrons and of neutrons with mean energy of 0.04; 0.09; 0.35; 0.85 and 14.7 MeV as well as by 60Co gamma-rays. The data were fitted to the linear and linear-quadratic relations. The neutrons of low and intermediate energies showed the linear dependence on the dose, 14.7 MeV neutrons and gamma-rays--a linear-quadratic one, whereas the data obtained with 0.85 MeV neutrons fitted well the both models. Terminal and interstitial deletions produced by both gamma-rays and neutrons showed different dependencies upon the dose. Some qualitative pecularities of aberration spectra were found in the experiments with neutrons as compared with the data on gamma-irradiation: the ratio of exchanges to fragments was greater, and aberrations of chromatid type were produced. The specially designed experiments and calculations showed that the last effect was not connected with induced radioactivity."}
{"id": "PMID:467977", "title": "[Spontaneous level of sister chromosome exchanges and their distribution in human cells].", "content": "Blood of practically healthy donors of both sexes (27 females and 23 males) was cultured under the standard conditions during 96 hours. Bromodeoxyuridine (BUdR) was added at the final concentration of 10 mkg/ml 28 hours before harvesting. The slides were stained with acridine orange and Giemsa for differential staining of chromatids. In each culture sister chromatid exchanges (SCE) were analysed in 50 cells, and the part of cells undergoing the first, second and third mitoses at the time of harvesting, was calculated. According to the mean number of SCE per cell, the distribution of individuals was consistent with the normal law, the mean being 6.525 and standard deviation--0.956. A significant heterogeneity in the speed of cell cycle of cultures was observed. The coefficient of variation for the part of cells undergoing the first mitosis was 50%, for the cells in the second mitosis--15%, and for the cells in the third mitosis--154%. Correlation analysis showed a positive dependence of the mean level of SCF upon the age of a donor and upon the part of cells in the second mitosis in this individual. No reliable correlation of the SCE level with the donor's sex was observed. The distribution of cells, obtained from the culture of one individual, was best approximated by beta-distribution, and the distribution of cells obtained from the cultures of different individuals--by gamma-distribution. In both there was obtained a satisfactory approximation by Pearson's distribution of the 1 type, and significant deviations were found from the normal, Poison's and the negative binomial distribution. The conditions were found of similarity of empirical distribution of SCE in cells to the normal one. For that, it is not the value of SCE for a separate cell that should be used as a unit of measurement, but the mean from the values of frequencies for 5-10 cells. Hence, it was shown that for the evaluation of the mean frequency of SCE with the precision of 1 exchange in separate individuals it is necessary to analyse 40 cells, and to observe the 15% increase of spontaneous SCE level under the action of deleterious factors--8 individuals are enough to analyse.", "contents": "[Spontaneous level of sister chromosome exchanges and their distribution in human cells]. Blood of practically healthy donors of both sexes (27 females and 23 males) was cultured under the standard conditions during 96 hours. Bromodeoxyuridine (BUdR) was added at the final concentration of 10 mkg/ml 28 hours before harvesting. The slides were stained with acridine orange and Giemsa for differential staining of chromatids. In each culture sister chromatid exchanges (SCE) were analysed in 50 cells, and the part of cells undergoing the first, second and third mitoses at the time of harvesting, was calculated. According to the mean number of SCE per cell, the distribution of individuals was consistent with the normal law, the mean being 6.525 and standard deviation--0.956. A significant heterogeneity in the speed of cell cycle of cultures was observed. The coefficient of variation for the part of cells undergoing the first mitosis was 50%, for the cells in the second mitosis--15%, and for the cells in the third mitosis--154%. Correlation analysis showed a positive dependence of the mean level of SCF upon the age of a donor and upon the part of cells in the second mitosis in this individual. No reliable correlation of the SCE level with the donor's sex was observed. The distribution of cells, obtained from the culture of one individual, was best approximated by beta-distribution, and the distribution of cells obtained from the cultures of different individuals--by gamma-distribution. In both there was obtained a satisfactory approximation by Pearson's distribution of the 1 type, and significant deviations were found from the normal, Poison's and the negative binomial distribution. The conditions were found of similarity of empirical distribution of SCE in cells to the normal one. For that, it is not the value of SCE for a separate cell that should be used as a unit of measurement, but the mean from the values of frequencies for 5-10 cells. Hence, it was shown that for the evaluation of the mean frequency of SCE with the precision of 1 exchange in separate individuals it is necessary to analyse 40 cells, and to observe the 15% increase of spontaneous SCE level under the action of deleterious factors--8 individuals are enough to analyse."}
{"id": "PMID:467978", "title": "Summary and critique of the new NIH guidelines for recombinant DNA research.", "content": "New NIH Guidelines for research involving recombinant DNA (R-DNA) molecules were issued on December 15, 1978. These are composed of four main parts, the first defining R-DNA and specifying prohibitions and exemptions, the second describing physical and biological containment, the third assigning the containment levels for many R-DNA experiments, and the fourth detailing the roles and responsibilities of the investigator, research institutions and NIH. Although the new Guidelines reduce restrictions, principally on those R-DNA experiments that use Escherichia coli K-12 host-vector systems, and exempt from the Guidelines several classes of experiments on prokaryotes that naturally exchange their DNA, most of their provisions are unjustified by the present assessment of the absence of any practical risks; many totally innocuous experiments are unnecessarily restricted and even virtually prohibited mainly because no host-vector systems were officially certified. The term Guidelines is a misnomer since they are mandatory regulations, even without any statutory basis. They impose large but unnecessary bureaucratic burdens on scientists, research institutions, research committees and NIH, and represent unwarranted censorship of basic research, which is antithetical to the creativity of human thought, thus posing serious dangers to the traditional freedom of inquiry.", "contents": "Summary and critique of the new NIH guidelines for recombinant DNA research. New NIH Guidelines for research involving recombinant DNA (R-DNA) molecules were issued on December 15, 1978. These are composed of four main parts, the first defining R-DNA and specifying prohibitions and exemptions, the second describing physical and biological containment, the third assigning the containment levels for many R-DNA experiments, and the fourth detailing the roles and responsibilities of the investigator, research institutions and NIH. Although the new Guidelines reduce restrictions, principally on those R-DNA experiments that use Escherichia coli K-12 host-vector systems, and exempt from the Guidelines several classes of experiments on prokaryotes that naturally exchange their DNA, most of their provisions are unjustified by the present assessment of the absence of any practical risks; many totally innocuous experiments are unnecessarily restricted and even virtually prohibited mainly because no host-vector systems were officially certified. The term Guidelines is a misnomer since they are mandatory regulations, even without any statutory basis. They impose large but unnecessary bureaucratic burdens on scientists, research institutions, research committees and NIH, and represent unwarranted censorship of basic research, which is antithetical to the creativity of human thought, thus posing serious dangers to the traditional freedom of inquiry."}
{"id": "PMID:467979", "title": "Nomenclature of transposable elements in prokaryotes.", "content": "Transposable elements are defined as specific DNA segments that can repeatedly insert into a few or many sites in a genome. They are classified as simple IS elements, more complex Tn transposons and self-replicating episomes. Definitions and nomenclature rules for these three classes of prokaryotic transposable elements are specified.", "contents": "Nomenclature of transposable elements in prokaryotes. Transposable elements are defined as specific DNA segments that can repeatedly insert into a few or many sites in a genome. They are classified as simple IS elements, more complex Tn transposons and self-replicating episomes. Definitions and nomenclature rules for these three classes of prokaryotic transposable elements are specified."}
{"id": "PMID:467980", "title": "Office management of cardiac disease in the diabetic.", "content": "Atherosclerotic vascular disease is very common in diabetic patients. It often occurs at an earlier age and is more severe than in nondiabetic individuals. The medical management of cardiac disease in diabetics is much the same as in nondiabetics. Risk factors such as obesity, hypertension, and hyperlipidemia must be vigorously treated, and smoking should be restricted.", "contents": "Office management of cardiac disease in the diabetic. Atherosclerotic vascular disease is very common in diabetic patients. It often occurs at an earlier age and is more severe than in nondiabetic individuals. The medical management of cardiac disease in diabetics is much the same as in nondiabetics. Risk factors such as obesity, hypertension, and hyperlipidemia must be vigorously treated, and smoking should be restricted."}
{"id": "PMID:467981", "title": "Herpes zoster: a geriatric disease.", "content": "Both the incidence and the severity of herpes zoster increase with advancing age. Postherpetic neuralgia may persist for more than a year. Treatment is usually symptomatic, and early corticosteroid therapy should be considered in patients over age 50. Psychotropic drugs also may be indicated to overcome depression.", "contents": "Herpes zoster: a geriatric disease. Both the incidence and the severity of herpes zoster increase with advancing age. Postherpetic neuralgia may persist for more than a year. Treatment is usually symptomatic, and early corticosteroid therapy should be considered in patients over age 50. Psychotropic drugs also may be indicated to overcome depression."}
{"id": "PMID:467982", "title": "Appendicitis in the elderly: learn to discern the untypical picture.", "content": "Symptoms and signs of appendicitis in the elderly may be vague and referable to other abdominal diseases. Diagnosis is often delayed or missed, leading to a prolonged preoperative period and increased morbidity and mortality. In this study of elderly appendicitis patients, we noted a high incidence of ruptured appendixes. At least 80% of these patients could have been operated on earlier.", "contents": "Appendicitis in the elderly: learn to discern the untypical picture. Symptoms and signs of appendicitis in the elderly may be vague and referable to other abdominal diseases. Diagnosis is often delayed or missed, leading to a prolonged preoperative period and increased morbidity and mortality. In this study of elderly appendicitis patients, we noted a high incidence of ruptured appendixes. At least 80% of these patients could have been operated on earlier."}
{"id": "PMID:467983", "title": "When to suspect spinal cord lesions in the elderly.", "content": "Motor or sensory disturbance in the lower extremities--sometimes with gait disturbance--is the most common symptom of spinal cord disease. History and examination may localize the lesion, but spine x-rays and myelography usually are necessary to determine its cause. The most common causes of cord compression in the elderly are spondylosis and neoplasm. Compressive lesions are usually treated by surgery. Once compression has been excluded and diagnosis established, treatment should be directed toward the underlying disorder.", "contents": "When to suspect spinal cord lesions in the elderly. Motor or sensory disturbance in the lower extremities--sometimes with gait disturbance--is the most common symptom of spinal cord disease. History and examination may localize the lesion, but spine x-rays and myelography usually are necessary to determine its cause. The most common causes of cord compression in the elderly are spondylosis and neoplasm. Compressive lesions are usually treated by surgery. Once compression has been excluded and diagnosis established, treatment should be directed toward the underlying disorder."}
{"id": "PMID:467984", "title": "Rehabilitating the geriatric patient: potential and limitations.", "content": "Rehabilitation of the elderly can reverse the trend toward greater dependence and escalating costs if the staff is specially trained.", "contents": "Rehabilitating the geriatric patient: potential and limitations. Rehabilitation of the elderly can reverse the trend toward greater dependence and escalating costs if the staff is specially trained."}
{"id": "PMID:468066", "title": "Variations in the incidence and the spatial distribution of patients with primary acute pancreatitis in Nottingham 1969-76.", "content": "During the eight year period 1969-76, 214 patients with primary acute pancreatitis were admitted to Nottingham hospitals from the Nottingham defined population area (the City of Nottingham and the four adjacent urban districts, which had a population of 469,720 in 1971). There was considerable variation in both the incidence and distribution of the disease within the study area and the crude average incidence rates ranged between 31.8 and 388.7 per million. Six of the eight wards with rates in excess of 200 per million were contiguous with similar wards; together these formed a U-shaped area extending from the city centre to the eastern boundary of the study area. These findings could not be accounted for by the age structure of the population or its social class structure. Although the Nottingham defined population area is relatively small (147 km2 or 57 sq. miles), it is subdivided into distinct and fixed domestic water supply areas. The distribution of patients among the six water supply areas showed that the Burton Joyce supply area, which is a particularly 'hard' water, contained significantly more patients than could have occurred by chance (P less than 0.002). This investigation of some social and geographical factors suggests that the chemical composition of the domestic water supply may affect the distribution of this disease within this particular urban area. This concentration of patients in the Burton Joyce water supply area suggests that some, as yet unidentified, property of the water supply may be an aetiological factor.", "contents": "Variations in the incidence and the spatial distribution of patients with primary acute pancreatitis in Nottingham 1969-76. During the eight year period 1969-76, 214 patients with primary acute pancreatitis were admitted to Nottingham hospitals from the Nottingham defined population area (the City of Nottingham and the four adjacent urban districts, which had a population of 469,720 in 1971). There was considerable variation in both the incidence and distribution of the disease within the study area and the crude average incidence rates ranged between 31.8 and 388.7 per million. Six of the eight wards with rates in excess of 200 per million were contiguous with similar wards; together these formed a U-shaped area extending from the city centre to the eastern boundary of the study area. These findings could not be accounted for by the age structure of the population or its social class structure. Although the Nottingham defined population area is relatively small (147 km2 or 57 sq. miles), it is subdivided into distinct and fixed domestic water supply areas. The distribution of patients among the six water supply areas showed that the Burton Joyce supply area, which is a particularly 'hard' water, contained significantly more patients than could have occurred by chance (P less than 0.002). This investigation of some social and geographical factors suggests that the chemical composition of the domestic water supply may affect the distribution of this disease within this particular urban area. This concentration of patients in the Burton Joyce water supply area suggests that some, as yet unidentified, property of the water supply may be an aetiological factor."}
{"id": "PMID:468067", "title": "Chronic persistent hepatitis: hepatitis B virus markers and histological follow-up.", "content": "Twenty-six untreated patients with chronic persistent hepatitis were followed prospectively for one to 17 years (mean 5.6 years). The patients developed no clinical features of chronic liver disease. Raised serum transaminase levels were usually, but not consistently, the only biochemical abnormality; gamma globulin values were normal. Serum markers of past or present hepatitis B infection were found initially in 14 patients: another two developed markers during their follow-up. Nine patients progressed to a mild or moderate chronic active hepatitis as shown by serial needle liver biopsies but there was no evidence of cirrhosis. This progression was not associated with any clinical or biochemical deterioration. Seven of these patients had presented with insidious symptoms, seven had serum markers of hepatitis B infection, and the four who were HBsAg positive had relatively lower serum HBsAg concentrations than did those patients who continued with chronic persistent hepatitis.", "contents": "Chronic persistent hepatitis: hepatitis B virus markers and histological follow-up. Twenty-six untreated patients with chronic persistent hepatitis were followed prospectively for one to 17 years (mean 5.6 years). The patients developed no clinical features of chronic liver disease. Raised serum transaminase levels were usually, but not consistently, the only biochemical abnormality; gamma globulin values were normal. Serum markers of past or present hepatitis B infection were found initially in 14 patients: another two developed markers during their follow-up. Nine patients progressed to a mild or moderate chronic active hepatitis as shown by serial needle liver biopsies but there was no evidence of cirrhosis. This progression was not associated with any clinical or biochemical deterioration. Seven of these patients had presented with insidious symptoms, seven had serum markers of hepatitis B infection, and the four who were HBsAg positive had relatively lower serum HBsAg concentrations than did those patients who continued with chronic persistent hepatitis."}
{"id": "PMID:468068", "title": "Chronic oral arsenic intoxication as a possible aetiological factor in idiopathic portal hypertension (non-cirrhotic portal fibrosis) in India.", "content": "Estimates were made of the arsenic concentration in liver specimens from nine patients having idiopathic portal hypertension (IP), and in four livers these were found to be significantly higher than those in patients with cirrhosis and in control subjects. The splenovenogram revealed extensive portosystemic collateral circulation. Corrected sinusoidal pressure and blood flow studies showed higher levels in four patients than in normal subjects. Microscopic examination of liver tissues revealed periportal fibrosis. The higher hepatic arsenic levels that were found were due to the inadvertent drinking of water contaminated with arsenic, adulterated opium, and indigenous medicines. A history of opium intake was not forthcoming but two patients had drunk water contaminated with arsenic and two others had taken bhasams (Ayurvedic medicines prepared by repeated oxidation of ores). Though the aetiology of idiopathic portal hypertension is not known, it is possible that arsenic intake may be one of the factors.", "contents": "Chronic oral arsenic intoxication as a possible aetiological factor in idiopathic portal hypertension (non-cirrhotic portal fibrosis) in India. Estimates were made of the arsenic concentration in liver specimens from nine patients having idiopathic portal hypertension (IP), and in four livers these were found to be significantly higher than those in patients with cirrhosis and in control subjects. The splenovenogram revealed extensive portosystemic collateral circulation. Corrected sinusoidal pressure and blood flow studies showed higher levels in four patients than in normal subjects. Microscopic examination of liver tissues revealed periportal fibrosis. The higher hepatic arsenic levels that were found were due to the inadvertent drinking of water contaminated with arsenic, adulterated opium, and indigenous medicines. A history of opium intake was not forthcoming but two patients had drunk water contaminated with arsenic and two others had taken bhasams (Ayurvedic medicines prepared by repeated oxidation of ores). Though the aetiology of idiopathic portal hypertension is not known, it is possible that arsenic intake may be one of the factors."}
{"id": "PMID:468069", "title": "Endogenous prostaglandins in peptic ulcer disease.", "content": "Plasma concentrations of prostaglandins E and F have been measured by radioimmunoassay in patients undergoing diagnostic upper intestinal endoscopy. The results fail to support a previously reported deficiency of plasma PGE in duodenal ulcer patients. Plasma prostaglandin concentrations failed to correlate with the parameters of gastric secretion studied; and were unaffected by histamine H2-receptor blockade or the activity of duodenal ulceration. During combined pentagastrin and insulin secretory studies there was a significant correlation between the outputs of PGE and acid into gastric juice.", "contents": "Endogenous prostaglandins in peptic ulcer disease. Plasma concentrations of prostaglandins E and F have been measured by radioimmunoassay in patients undergoing diagnostic upper intestinal endoscopy. The results fail to support a previously reported deficiency of plasma PGE in duodenal ulcer patients. Plasma prostaglandin concentrations failed to correlate with the parameters of gastric secretion studied; and were unaffected by histamine H2-receptor blockade or the activity of duodenal ulceration. During combined pentagastrin and insulin secretory studies there was a significant correlation between the outputs of PGE and acid into gastric juice."}
{"id": "PMID:468070", "title": "Rectal absorption of short chain fatty acids in the absence of chloride.", "content": "The absorption of short chain fatty acids by the human rectum in the absence of chloride has been studied using a dialysis bag technique. Absorption rates of sodium, water, and short chain fatty acids were unchanged on replacing chloride with sulphate. Final concentrations of bicarbonate were 35.4 +/- 3.4 mmol/l in the presence of chloride and 37.1 +/- 5.2 mmol/l in the absence of chloride. The rates of movement of bicarbonate into the dialysates were similar. If bicarbonate is secreted in exchange for chloride then it also appears to be secreted in exchange for absorbed short chain fatty acids.", "contents": "Rectal absorption of short chain fatty acids in the absence of chloride. The absorption of short chain fatty acids by the human rectum in the absence of chloride has been studied using a dialysis bag technique. Absorption rates of sodium, water, and short chain fatty acids were unchanged on replacing chloride with sulphate. Final concentrations of bicarbonate were 35.4 +/- 3.4 mmol/l in the presence of chloride and 37.1 +/- 5.2 mmol/l in the absence of chloride. The rates of movement of bicarbonate into the dialysates were similar. If bicarbonate is secreted in exchange for chloride then it also appears to be secreted in exchange for absorbed short chain fatty acids."}
{"id": "PMID:468071", "title": "Effect of long-term cimetidine on gastric acid secretion, serum gastrin, and gastric emptying.", "content": "Gastric acid secretion, gastric emptying, fasting serum gastrin and the serum gastrin response to a meal were measured in duodenal ulcer patients before, and at least five days after completing prolonged treatment with cimetidine (1 or 2 g/day for four or eight weeks followed by 600 mg twice daily for six months). Fasting serum gastrin and the gastrin response were also measured during treatment. Compared with pretreatment values, fasting serum gastrin concentrations were raised both during and five to 31 days after stopping treatment (P less than or equal to 0.02). The integrated gastrin response and the rate of gastric emptying of the solid component of the meal were increased during treatment (P less than 0.001 and P less than 0.002 respectively) but returned to pretreatment levels after stopping therapy. No significant changes were observed in the basal or maximal acid outputs or the rate of emptying of the liquid component of the meal. The results imply that the hypergastrinaemia associated with long-term cimetidine therapy does not result in increased gastric acid secretion.", "contents": "Effect of long-term cimetidine on gastric acid secretion, serum gastrin, and gastric emptying. Gastric acid secretion, gastric emptying, fasting serum gastrin and the serum gastrin response to a meal were measured in duodenal ulcer patients before, and at least five days after completing prolonged treatment with cimetidine (1 or 2 g/day for four or eight weeks followed by 600 mg twice daily for six months). Fasting serum gastrin and the gastrin response were also measured during treatment. Compared with pretreatment values, fasting serum gastrin concentrations were raised both during and five to 31 days after stopping treatment (P less than or equal to 0.02). The integrated gastrin response and the rate of gastric emptying of the solid component of the meal were increased during treatment (P less than 0.001 and P less than 0.002 respectively) but returned to pretreatment levels after stopping therapy. No significant changes were observed in the basal or maximal acid outputs or the rate of emptying of the liquid component of the meal. The results imply that the hypergastrinaemia associated with long-term cimetidine therapy does not result in increased gastric acid secretion."}
{"id": "PMID:468072", "title": "Morphology of experimental antibiotic-associated enterocolitis in the hamster: a model for human pseudomembranous colitis and antibiotic-associated diarrhoea.", "content": "The morphology of antibiotic-associated enterocolitis in the hamster is described and compared with human antibiotic-associated pseudomembranous colitis. It is shown to be a caecal disease with proliferative mucosal changes and in this respect unlike the human counterpart. The bacteriology and toxicology, however, are identical. In addition, mucosal changes are described in animals on antibiotics but without established enterocolitis. As a result we suggest that there may be a spectrum of human disease ranging from mild antibiotic-associated diarrhoea to established pseudomembranous colitis. Therefore, despite the morphological variation, the hamster remains a good model for investigating the pathogenesis of pseudomembranous colitis and antibiotic-associated enteropathy in general.", "contents": "Morphology of experimental antibiotic-associated enterocolitis in the hamster: a model for human pseudomembranous colitis and antibiotic-associated diarrhoea. The morphology of antibiotic-associated enterocolitis in the hamster is described and compared with human antibiotic-associated pseudomembranous colitis. It is shown to be a caecal disease with proliferative mucosal changes and in this respect unlike the human counterpart. The bacteriology and toxicology, however, are identical. In addition, mucosal changes are described in animals on antibiotics but without established enterocolitis. As a result we suggest that there may be a spectrum of human disease ranging from mild antibiotic-associated diarrhoea to established pseudomembranous colitis. Therefore, despite the morphological variation, the hamster remains a good model for investigating the pathogenesis of pseudomembranous colitis and antibiotic-associated enteropathy in general."}
{"id": "PMID:468073", "title": "Colonic myoelectrical activity in man: comparison of recording techniques and methods of analysis.", "content": "Both monopolar and bipolar recordings of human colonic myoelectrical activity have been made on 29 occasions from implanted serosal electrodes attached to either the ascending colon or transverse colon. Visual and computer analysis of the signals were made and the incidences of the two electrical rhythms present in the colon were compared for the two recording techniques, recording sites, and methods of analysis. The percentage incidence of both rhythms was similar for the mono and bipolar recording techniques in both parts of the colon. The incidence of the two rhythms in the transverse colon was approximately twice that recorded in the ascending colon, this being true for both recording techniques and methods of analysis, but neither was detected for more than 50% of the time. It is concluded that the methods of data analysis probably account for the differing incidences of electrical activity in the human colon that have been reported previously.", "contents": "Colonic myoelectrical activity in man: comparison of recording techniques and methods of analysis. Both monopolar and bipolar recordings of human colonic myoelectrical activity have been made on 29 occasions from implanted serosal electrodes attached to either the ascending colon or transverse colon. Visual and computer analysis of the signals were made and the incidences of the two electrical rhythms present in the colon were compared for the two recording techniques, recording sites, and methods of analysis. The percentage incidence of both rhythms was similar for the mono and bipolar recording techniques in both parts of the colon. The incidence of the two rhythms in the transverse colon was approximately twice that recorded in the ascending colon, this being true for both recording techniques and methods of analysis, but neither was detected for more than 50% of the time. It is concluded that the methods of data analysis probably account for the differing incidences of electrical activity in the human colon that have been reported previously."}
{"id": "PMID:468074", "title": "Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies.", "content": "Suspensions of isolated colonic epithelial cells (colonocytes) have been obtained from rats by incubating everted lengths of colon with EDTA at 37 degrees C in Krebs-Henseleit saline from which calcium was omitted and containing 0.25% (w/v) bovine serum albumin. Measurements of oxygen consumption and lactate production by cell suspensions indicate that they are metabolically active for at least one hour. The method has been modified for the preparation of isolated epithelial cells from the human colon by including an enzyme digestion step and by increasing the concentration of EDTA to 10 mM. Human colonocytes have been obtained either from normal mucosa (ascending and descending colon) or from the mucosa in ulcerative colitis (descending colon). Oxygen consumption of human cell suspensions is lower than in the rat but in colonocytes from both species the rate is increased by glucose and by n-butyrate, a normal constituent of the colonic lumen. The method yields metabolically active cell suspensions from diseased colonic mucosa and promises to be of value for biochemical studies of ulcerative colitis.", "contents": "Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies. Suspensions of isolated colonic epithelial cells (colonocytes) have been obtained from rats by incubating everted lengths of colon with EDTA at 37 degrees C in Krebs-Henseleit saline from which calcium was omitted and containing 0.25% (w/v) bovine serum albumin. Measurements of oxygen consumption and lactate production by cell suspensions indicate that they are metabolically active for at least one hour. The method has been modified for the preparation of isolated epithelial cells from the human colon by including an enzyme digestion step and by increasing the concentration of EDTA to 10 mM. Human colonocytes have been obtained either from normal mucosa (ascending and descending colon) or from the mucosa in ulcerative colitis (descending colon). Oxygen consumption of human cell suspensions is lower than in the rat but in colonocytes from both species the rate is increased by glucose and by n-butyrate, a normal constituent of the colonic lumen. The method yields metabolically active cell suspensions from diseased colonic mucosa and promises to be of value for biochemical studies of ulcerative colitis."}
{"id": "PMID:468075", "title": "High flow oxygen therapy for pneumatosis coli.", "content": "Symptomatic and radiological resolution of pneumatosis coli was achieved by intermittent high flow oxygen therapy in five patients. In each case the extent of the disease was defined by colonoscopy and contrast radiography before treatment. Despite the confirmation of pneumocyst resolution, recurrence of colonic gas cysts was noted in two patients at six months and one year after treatment. Bacteriological studies indicated that resolution of the disease, induced by oxygen therapy, was not associated with eradication of anerobic bacteria from stool and colonic mucosa. The clinical features and response to treatment of this group of patients are discussed, with particular reference to previously reported methods of oxygen administration.", "contents": "High flow oxygen therapy for pneumatosis coli. Symptomatic and radiological resolution of pneumatosis coli was achieved by intermittent high flow oxygen therapy in five patients. In each case the extent of the disease was defined by colonoscopy and contrast radiography before treatment. Despite the confirmation of pneumocyst resolution, recurrence of colonic gas cysts was noted in two patients at six months and one year after treatment. Bacteriological studies indicated that resolution of the disease, induced by oxygen therapy, was not associated with eradication of anerobic bacteria from stool and colonic mucosa. The clinical features and response to treatment of this group of patients are discussed, with particular reference to previously reported methods of oxygen administration."}
{"id": "PMID:468076", "title": "Absorption studies in patients six to 10 years after construction of ileostomy reservoirs.", "content": "The absorption of fat, bile salts, and vitamin B12 was studied in 14 proctocolectomised patients six to 10 years after construction of a continent ileostomy. All patients were in excellent health and displayed no signs of malnutrition. Schilling test disclosed subnormal absorption of vitamin B12 in one patient and borderline values in five. Faecal loss of bile acid was increased in 12 patients and fat malabsorption occurred in two. Except for slightly pathological liver tests in two patients, routine laboratory tests, including plasma concentration of vitamin B12 and folic acid, were normal. There were no signs of sodium or potassium depletion.", "contents": "Absorption studies in patients six to 10 years after construction of ileostomy reservoirs. The absorption of fat, bile salts, and vitamin B12 was studied in 14 proctocolectomised patients six to 10 years after construction of a continent ileostomy. All patients were in excellent health and displayed no signs of malnutrition. Schilling test disclosed subnormal absorption of vitamin B12 in one patient and borderline values in five. Faecal loss of bile acid was increased in 12 patients and fat malabsorption occurred in two. Except for slightly pathological liver tests in two patients, routine laboratory tests, including plasma concentration of vitamin B12 and folic acid, were normal. There were no signs of sodium or potassium depletion."}
{"id": "PMID:468077", "title": "Ultrastructural analysis of plasma cells in coeliac patients.", "content": "By accurate morphometry the length of the rough endoplasmic reticulum (RER) was estimated in electron micrographs of a population of jejunal plasma cells in biopsies obtained from normal volunteers, untreated coeliac disease patients, and coeliac disease patients treated for one year on a gluten free diet. Increase in length or hypertrophy of the endoplasmic reticulum is an indication of increased protein synthesis of the cell (Ghadially, 1977). An increase compared with normal length was demonstrated in the mean length of the plasma cell rough endoplasmic reticulum in plasma cells obtained from both groups of coeliac patients. This estimate of increased protein production--in this case immunoglobulin--indicates an increase in the immunological activity of plasma cells even after treatment and suggests not only a reaction to dietary gluten but probably to other antigens also.", "contents": "Ultrastructural analysis of plasma cells in coeliac patients. By accurate morphometry the length of the rough endoplasmic reticulum (RER) was estimated in electron micrographs of a population of jejunal plasma cells in biopsies obtained from normal volunteers, untreated coeliac disease patients, and coeliac disease patients treated for one year on a gluten free diet. Increase in length or hypertrophy of the endoplasmic reticulum is an indication of increased protein synthesis of the cell (Ghadially, 1977). An increase compared with normal length was demonstrated in the mean length of the plasma cell rough endoplasmic reticulum in plasma cells obtained from both groups of coeliac patients. This estimate of increased protein production--in this case immunoglobulin--indicates an increase in the immunological activity of plasma cells even after treatment and suggests not only a reaction to dietary gluten but probably to other antigens also."}
{"id": "PMID:468078", "title": "Effect of 16,16-dimethyl prostaglandin E2 on the gastric mucosal barrier.", "content": "16,16-dimethyl prostaglandin E2 (16DM) can protect the gastric mucosa from injury and yet apparently damages the gastric mucosal barrier. The effect on the gastric mucosal barrier of 16DM (26.2 micromol/l), a dose 50 times the ED50 for inhibition of acid secretion, was investigated in Heidenhain pouches in four dogs by measuring plasma shedding from the pouches after the topical application of histamine (2.7 mmol/l) and ionic fluxes. The results were compared with those using 30% ethanol, a known barrier breaker. The topical application of histamine after three hours' perfusion with 30% ethanol led to plasma shedding at a rate of 7.5 (+/-2.6) ml/h, which was significantly greater than the rate of 1.2 (+/-1.4) ml/h after three hours' perfusion with 16DM and of 1.5 (+/-1.7) ml/h in the control group. Ethanol also caused an increase in the flux of H+, Na+, and Cl-, indicating an increase in mucosal permeability, whereas 16DM increased the flux of Na+ and Cl- but not of H+. It is concluded that 16DM does not damage the gastric mucosal barrier but stimulates the secretion of fluid containing Na+ and Cl-.", "contents": "Effect of 16,16-dimethyl prostaglandin E2 on the gastric mucosal barrier. 16,16-dimethyl prostaglandin E2 (16DM) can protect the gastric mucosa from injury and yet apparently damages the gastric mucosal barrier. The effect on the gastric mucosal barrier of 16DM (26.2 micromol/l), a dose 50 times the ED50 for inhibition of acid secretion, was investigated in Heidenhain pouches in four dogs by measuring plasma shedding from the pouches after the topical application of histamine (2.7 mmol/l) and ionic fluxes. The results were compared with those using 30% ethanol, a known barrier breaker. The topical application of histamine after three hours' perfusion with 30% ethanol led to plasma shedding at a rate of 7.5 (+/-2.6) ml/h, which was significantly greater than the rate of 1.2 (+/-1.4) ml/h after three hours' perfusion with 16DM and of 1.5 (+/-1.7) ml/h in the control group. Ethanol also caused an increase in the flux of H+, Na+, and Cl-, indicating an increase in mucosal permeability, whereas 16DM increased the flux of Na+ and Cl- but not of H+. It is concluded that 16DM does not damage the gastric mucosal barrier but stimulates the secretion of fluid containing Na+ and Cl-."}
{"id": "PMID:468079", "title": "Lipid composition of bile in diabetics and obesity-matched controls.", "content": "Duodenal bile from 27 diabetes was compared with samples from healthy subjects matched for age, sex, and body mass index. Cholesterol saturation and the molar percentages of bile acids, phospholipids, and cholesterol were not significantly different. Most bile samples were supersaturated in both groups. The maturity onset diabetics who were almost all obese had more saturated bile than the slimmer juvenile onset patients. Body fatness and plasma triglyceride levels were both positively correlated with the cholesterol saturation of bile in the controls but not in the diabetics. Bile was less concentrated in female diabetics than in controls, which is consistent with impaired gallbladder emptying. It is possible that the increased prevalence of gallstones in diabetics is due not so much to diabetes itself as to the frequently associated obesity.", "contents": "Lipid composition of bile in diabetics and obesity-matched controls. Duodenal bile from 27 diabetes was compared with samples from healthy subjects matched for age, sex, and body mass index. Cholesterol saturation and the molar percentages of bile acids, phospholipids, and cholesterol were not significantly different. Most bile samples were supersaturated in both groups. The maturity onset diabetics who were almost all obese had more saturated bile than the slimmer juvenile onset patients. Body fatness and plasma triglyceride levels were both positively correlated with the cholesterol saturation of bile in the controls but not in the diabetics. Bile was less concentrated in female diabetics than in controls, which is consistent with impaired gallbladder emptying. It is possible that the increased prevalence of gallstones in diabetics is due not so much to diabetes itself as to the frequently associated obesity."}
{"id": "PMID:468080", "title": "Amino acid control mechanism for bacitracin formation by Bacillus licheniformis.", "content": "Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino acids. This indicate that they may have differences in their amino acid control mechanism.", "contents": "Amino acid control mechanism for bacitracin formation by Bacillus licheniformis. Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino acids. This indicate that they may have differences in their amino acid control mechanism."}
{"id": "PMID:468081", "title": "Effect of bacterial polysaccharides on the growth of Gaeumannomyces graminis var. tritici and wheat roots.", "content": "Agrobacterium sp. and related species which in the soil and in the rhizosphere of wheat accompany the fungus Gaemannomyces graminis var. tritici and cause take-all of the wheat roots produced polysaccharides in pure cultures (glucans, mannoglucans and galactomannoglucans). These polysaccharides were utilized better by the mycelium of G. graminis than glucose and polysaccharides of plant origin that occurred on the surface of wheat roots (the so-called mucigel). At lower concentrations these bacterial polysaccharides stimulated growth of wheat roots, higher concentrations (more than 0.1%) were inhibitory. Bacteria inoculated on the surface of wheat first inhibited and then stimulated the development of the plants and their growth. Changes in the growth rate of wheat, the rhizosphere of which was colonized by bacteria simultaneously with the fungus G. graminis and also some changes in the course of the disease of wheat roots caused by the fungus can be explained by the inhibitory or stimulatory effect of polysaccharides of accompanying bacteria.", "contents": "Effect of bacterial polysaccharides on the growth of Gaeumannomyces graminis var. tritici and wheat roots. Agrobacterium sp. and related species which in the soil and in the rhizosphere of wheat accompany the fungus Gaemannomyces graminis var. tritici and cause take-all of the wheat roots produced polysaccharides in pure cultures (glucans, mannoglucans and galactomannoglucans). These polysaccharides were utilized better by the mycelium of G. graminis than glucose and polysaccharides of plant origin that occurred on the surface of wheat roots (the so-called mucigel). At lower concentrations these bacterial polysaccharides stimulated growth of wheat roots, higher concentrations (more than 0.1%) were inhibitory. Bacteria inoculated on the surface of wheat first inhibited and then stimulated the development of the plants and their growth. Changes in the growth rate of wheat, the rhizosphere of which was colonized by bacteria simultaneously with the fungus G. graminis and also some changes in the course of the disease of wheat roots caused by the fungus can be explained by the inhibitory or stimulatory effect of polysaccharides of accompanying bacteria."}
{"id": "PMID:468082", "title": "Studies on the frequencies of PGM1, PGM3 and Es-D types from hair roots in Japanese subjects and the determination of these types from old hair roots.", "content": "The frequencies in Japanese subjects are reported of hair roots type PGM1, PGM3, and Es-D, and the determination of these types from old hair roots. The gene frequencies were: PGM11, 0.762; PGM21, 0.230; PGM 71, 0.008; PGM13, 0.621; PGM23, 0.379; Es-D1, 0.625; and Es-D2, 0.375. The old hair roots were analysed after storage at 25 degrees C, 4 degrees C and -80 degrees C; the enzyme activities were detected and typed at 25 degrees C within PGM1 10 days, PGM3 4 days, and Es-D 4 days.", "contents": "Studies on the frequencies of PGM1, PGM3 and Es-D types from hair roots in Japanese subjects and the determination of these types from old hair roots. The frequencies in Japanese subjects are reported of hair roots type PGM1, PGM3, and Es-D, and the determination of these types from old hair roots. The gene frequencies were: PGM11, 0.762; PGM21, 0.230; PGM 71, 0.008; PGM13, 0.621; PGM23, 0.379; Es-D1, 0.625; and Es-D2, 0.375. The old hair roots were analysed after storage at 25 degrees C, 4 degrees C and -80 degrees C; the enzyme activities were detected and typed at 25 degrees C within PGM1 10 days, PGM3 4 days, and Es-D 4 days."}
{"id": "PMID:468084", "title": "Frequencies of salivary genetic marker systems in the Japanese population and their application to forensic medicine.", "content": "Seven salivary polymorphic systems were studied using whole and parotid saliva from random Japanese individuals. The gene frequencies obtained were: Pa+ = 0.212, Pb1 = 1.000, Pb2 = 0, Pr1 = 0.763, Pr2 = 0.237, Db+ = 0.051, Pm+ = 0.409, Ph+ = 0.026 and Amy1v = 0.013, respectively. Based on these gene frequencies, the chances for exclusion of falsely alleged fathers were calculated. The chance of exclusion on the basis of five salivary polymorphic systems was 0.305. The combined chance of exclusion utilizing only blood, serum and red-cell enzyme polymorphic systems among the Japanese population was 0.919; however, by applying salivary polymorphic systems to the calculation, the total exclusion rose to 0.944.", "contents": "Frequencies of salivary genetic marker systems in the Japanese population and their application to forensic medicine. Seven salivary polymorphic systems were studied using whole and parotid saliva from random Japanese individuals. The gene frequencies obtained were: Pa+ = 0.212, Pb1 = 1.000, Pb2 = 0, Pr1 = 0.763, Pr2 = 0.237, Db+ = 0.051, Pm+ = 0.409, Ph+ = 0.026 and Amy1v = 0.013, respectively. Based on these gene frequencies, the chances for exclusion of falsely alleged fathers were calculated. The chance of exclusion on the basis of five salivary polymorphic systems was 0.305. The combined chance of exclusion utilizing only blood, serum and red-cell enzyme polymorphic systems among the Japanese population was 0.919; however, by applying salivary polymorphic systems to the calculation, the total exclusion rose to 0.944."}
{"id": "PMID:468086", "title": "Determination of phenotypes of esterases (Set) in fresh saliva and saliva stains by disc electrophoresis and the distribution of Set phenotypes in the Japanese population.", "content": "A rapid and simple disc electrophoretic technique for the determination of saliva esterase (Set) types is described. The frequency of Set types was F 16.4%, FS 49.8% and S 33.8%. The estimated gene frequency of Set-1F was 0.413 and of Set-1S was 0.587. An accurate determination of Set types was possible in 5--7 week-old saliva stains. This system may be useful in medicolegal applications.", "contents": "Determination of phenotypes of esterases (Set) in fresh saliva and saliva stains by disc electrophoresis and the distribution of Set phenotypes in the Japanese population. A rapid and simple disc electrophoretic technique for the determination of saliva esterase (Set) types is described. The frequency of Set types was F 16.4%, FS 49.8% and S 33.8%. The estimated gene frequency of Set-1F was 0.413 and of Set-1S was 0.587. An accurate determination of Set types was possible in 5--7 week-old saliva stains. This system may be useful in medicolegal applications."}
{"id": "PMID:468083", "title": "The significance of medicolegal autopsy in determining mode and cause of death.", "content": "A comparative examination has been made to evaluate decisions as to causes and modes of death among 436 consecutive medicolegal deaths before and after coming to autopsy. All available information was used. including police reports, in evaluating mode and cause of death prior to the autopsy. Among the women, the mode of death evaluation differed in 23.4% of the cases and the cause of death evaluation differed in 77.5% of the cases. Among the men, the mode of death evaluation differed in 23.4% of the cases and the cause of death evaluation differed in 46.9% of the cases. These figures were achieved, however, on the basis of the fact that in most cases the mode of death and cause of death were reported as unknown. After autopsy, the cause of death remained unknown in 3.3% of the females over the age of 2 years and in 4.2% of the male over the age of 2 years. No characteristic relationship was established between the differences found and the age groups. The investigation shows the importance of the medicolegal autopsy for correctly recording cases where the death is obscure.", "contents": "The significance of medicolegal autopsy in determining mode and cause of death. A comparative examination has been made to evaluate decisions as to causes and modes of death among 436 consecutive medicolegal deaths before and after coming to autopsy. All available information was used. including police reports, in evaluating mode and cause of death prior to the autopsy. Among the women, the mode of death evaluation differed in 23.4% of the cases and the cause of death evaluation differed in 77.5% of the cases. Among the men, the mode of death evaluation differed in 23.4% of the cases and the cause of death evaluation differed in 46.9% of the cases. These figures were achieved, however, on the basis of the fact that in most cases the mode of death and cause of death were reported as unknown. After autopsy, the cause of death remained unknown in 3.3% of the females over the age of 2 years and in 4.2% of the male over the age of 2 years. No characteristic relationship was established between the differences found and the age groups. The investigation shows the importance of the medicolegal autopsy for correctly recording cases where the death is obscure."}
{"id": "PMID:468092", "title": "[3 vaccines against influenza--which to choose].", "content": "Three types of a killed influenza vaccine are available in the Federal Republic of Germany: Whole-virus-vaccine, split-vaccine, subunit-vaccine. It is not only necessary to enforce the vaccination against influenza virus infection, but additionally to differentiate between the different types of vaccine in vaccinating children and adults with special risks.", "contents": "[3 vaccines against influenza--which to choose]. Three types of a killed influenza vaccine are available in the Federal Republic of Germany: Whole-virus-vaccine, split-vaccine, subunit-vaccine. It is not only necessary to enforce the vaccination against influenza virus infection, but additionally to differentiate between the different types of vaccine in vaccinating children and adults with special risks."}
{"id": "PMID:468087", "title": "Separation and identification of 9-chloro-10-methoxy (9-methoxy-10-chloro)hexadecanoic and octadecanoic acids in adipocere.", "content": "Two homologs of 9-chloro-10-methoxy(9-methoxy-10-chloro) fatty acids were found in adipocere from the human neonate, and identified as 9-chloro-10-methoxy(9-methoxy-10-chloro)hexadecanoic acid and 9-chloro-10-methoxy(9-methoxy-10-chloro)-octadecanoic acid. The chemical structures of these compounds were confirmed by thin-layer chromatography, gas-liquid chromatography, gas chromatography--mass spectrometry, nuclear magnetic resonance spectrometry and elemental analysis. The adipocere contained approximately 7.2% 9-chloro-10-methoxy(9-methoxy-10-chloro) fatty acids in the total fatty acids.", "contents": "Separation and identification of 9-chloro-10-methoxy (9-methoxy-10-chloro)hexadecanoic and octadecanoic acids in adipocere. Two homologs of 9-chloro-10-methoxy(9-methoxy-10-chloro) fatty acids were found in adipocere from the human neonate, and identified as 9-chloro-10-methoxy(9-methoxy-10-chloro)hexadecanoic acid and 9-chloro-10-methoxy(9-methoxy-10-chloro)-octadecanoic acid. The chemical structures of these compounds were confirmed by thin-layer chromatography, gas-liquid chromatography, gas chromatography--mass spectrometry, nuclear magnetic resonance spectrometry and elemental analysis. The adipocere contained approximately 7.2% 9-chloro-10-methoxy(9-methoxy-10-chloro) fatty acids in the total fatty acids."}
{"id": "PMID:468088", "title": "False positive reaction for carboxyhemoglobin in blood from liver tissue.", "content": "On spectrophotometric determination of carboxyhemoglobin in blood collected from the liver of three bodies at three days post-mortem, false positive results were found (5--15 per cent saturation), since samples of heart blood collected a few hours after death did not contain carboxyhemoglobin.", "contents": "False positive reaction for carboxyhemoglobin in blood from liver tissue. On spectrophotometric determination of carboxyhemoglobin in blood collected from the liver of three bodies at three days post-mortem, false positive results were found (5--15 per cent saturation), since samples of heart blood collected a few hours after death did not contain carboxyhemoglobin."}
{"id": "PMID:468094", "title": "[Pathogenesis of essential hypertension. Plasma noradrenaline, plasma renin and pressor effects of noradrenaline and angiotensin in normotensive patients and patients with essential hypertension].", "content": "Plasma noradrenaline, plasma renin and pressor action of exogenous noradrenaline and angiotensin in normotensive subjects and patients with essential hypertension. In normotensive subjects an inverse correlation was observed between the index of sympathetic nervous activity, the plasma noradrenaline concentration during physical exercise, and reactivity to exogenous noradrenaline. The relationship between the index of sympathetic nervous activity and reactivity to noradrenaline was invariably disturbed in age-matched patients with essential hypertension. A multiple regression analysis revealed a highly significant correlation between the combination of both factors and the height of mean arterial blood pressure (r = 0.91). The data suggest that both factors combined, sympathetic nervous activity and pressor response to noradrenaline, are an important determinant of the arterial blood pressure level. An inverse relationship could also be demonstrated between plasma renin concentration and pressor response to angiotensin II in normotensives and hypertensives. However, this relationship was unaltered in the hypertensives. Therefore angiotensin II does not appear to contribute directly to the high blood pressure.", "contents": "[Pathogenesis of essential hypertension. Plasma noradrenaline, plasma renin and pressor effects of noradrenaline and angiotensin in normotensive patients and patients with essential hypertension]. Plasma noradrenaline, plasma renin and pressor action of exogenous noradrenaline and angiotensin in normotensive subjects and patients with essential hypertension. In normotensive subjects an inverse correlation was observed between the index of sympathetic nervous activity, the plasma noradrenaline concentration during physical exercise, and reactivity to exogenous noradrenaline. The relationship between the index of sympathetic nervous activity and reactivity to noradrenaline was invariably disturbed in age-matched patients with essential hypertension. A multiple regression analysis revealed a highly significant correlation between the combination of both factors and the height of mean arterial blood pressure (r = 0.91). The data suggest that both factors combined, sympathetic nervous activity and pressor response to noradrenaline, are an important determinant of the arterial blood pressure level. An inverse relationship could also be demonstrated between plasma renin concentration and pressor response to angiotensin II in normotensives and hypertensives. However, this relationship was unaltered in the hypertensives. Therefore angiotensin II does not appear to contribute directly to the high blood pressure."}
{"id": "PMID:468095", "title": "[The animal experiment in toxicology. Current situation and further development].", "content": "In view of recent public reproaches against medical animal experimentation this article describes the present status of animal experimentation in the field of toxicology. Possible perspectives and the limitations of \"alternative\" methods are reviewed. Toxicological methods which are based on cell culture and metabolic in vitro-systems, as well as \"short-term-tests\" for mutagenicity and/or carcinogenicity are invaluable for screening purposes. However, a risk assessment, i.e., evaluation of hazard of chemical compounds to man, must be based on animal experiments. Further efforts must be directed towards improvement of extrapolation of toxicological results from one species to the other.", "contents": "[The animal experiment in toxicology. Current situation and further development]. In view of recent public reproaches against medical animal experimentation this article describes the present status of animal experimentation in the field of toxicology. Possible perspectives and the limitations of \"alternative\" methods are reviewed. Toxicological methods which are based on cell culture and metabolic in vitro-systems, as well as \"short-term-tests\" for mutagenicity and/or carcinogenicity are invaluable for screening purposes. However, a risk assessment, i.e., evaluation of hazard of chemical compounds to man, must be based on animal experiments. Further efforts must be directed towards improvement of extrapolation of toxicological results from one species to the other."}
{"id": "PMID:468089", "title": "The human distribution of some barbiturate sedatives in combination with miscellaneous CNS-active drugs.", "content": "This paper presents 14 cases in which the distribution of barbiturates and the hydroxylated metabolites in combination with miscellaneous CNS-active drugs was studied. In 7 of these cases the other drug present was methadone, and in the remainder dihydrocodeine, morphine, propoxyphene, amitriptyline, meprobamate, cyclizine and dipipanone, diphenhydramine and methaqualine. Amitriptyline, methadone, cyclizine and dipipanone, methaqualone and diphenhydramine appeared to modify the distribution of amylorbarbitone and quinalbarbitone. Likewise, the barbituates seemed to alter the distribution of amitriptyline, propoxyphene and, in one instance, methadone.", "contents": "The human distribution of some barbiturate sedatives in combination with miscellaneous CNS-active drugs. This paper presents 14 cases in which the distribution of barbiturates and the hydroxylated metabolites in combination with miscellaneous CNS-active drugs was studied. In 7 of these cases the other drug present was methadone, and in the remainder dihydrocodeine, morphine, propoxyphene, amitriptyline, meprobamate, cyclizine and dipipanone, diphenhydramine and methaqualine. Amitriptyline, methadone, cyclizine and dipipanone, methaqualone and diphenhydramine appeared to modify the distribution of amylorbarbitone and quinalbarbitone. Likewise, the barbituates seemed to alter the distribution of amitriptyline, propoxyphene and, in one instance, methadone."}
{"id": "PMID:468096", "title": "[Acetlysalicylic acid, protective antacid effect and lesions of the gastric mucosa. Effect of acetylsalicylic acid and an antacid drug (Gastropulgit Tabs) on the transmural electric potential in the human stomach].", "content": "Antacids are able to prevent acetylsalicylic acid-induced functional and morphological changes of the gastric mucosa. In order to test whether a new antacid in chewing-tablet form (Gastropulgit Tabs) might be able to protect the gastric mucosa from acetylsalicylic acid-induced functional changes similarly to liquid antacids the effect of this antacid on acetylsalicylic acid-induced changes of transmural gastric potential difference was measured in healthy volunteers. The decrease of transmural potential differences induced by 640 mg acetylsalicylic acid could be prevented by simultaneous addition of 2 tablets of Gastropulgit Tabs. Thus the antacid in tablet form is able-like liquid antacids-to protect the gastric mucosa against acetylsalicylic acid-induced functional changes.", "contents": "[Acetlysalicylic acid, protective antacid effect and lesions of the gastric mucosa. Effect of acetylsalicylic acid and an antacid drug (Gastropulgit Tabs) on the transmural electric potential in the human stomach]. Antacids are able to prevent acetylsalicylic acid-induced functional and morphological changes of the gastric mucosa. In order to test whether a new antacid in chewing-tablet form (Gastropulgit Tabs) might be able to protect the gastric mucosa from acetylsalicylic acid-induced functional changes similarly to liquid antacids the effect of this antacid on acetylsalicylic acid-induced changes of transmural gastric potential difference was measured in healthy volunteers. The decrease of transmural potential differences induced by 640 mg acetylsalicylic acid could be prevented by simultaneous addition of 2 tablets of Gastropulgit Tabs. Thus the antacid in tablet form is able-like liquid antacids-to protect the gastric mucosa against acetylsalicylic acid-induced functional changes."}
{"id": "PMID:468097", "title": "[Studies on the estimation of the postmortem interval. 9. Synthetic judgment of all sorts of cadaveric findings (author's transl)].", "content": "The authors have investigated the relationship between the postmortem interval and all sorts of cadaveric findings, summarizing the results of the previous reports, and have devised a cadaveric-phenomena diagram and a chart with which the postmortem interval can be estimated easily. The validity and utility of the diagram and the chart have been demonstrated by applying them to other autopsy cases than those from which they have been derived.", "contents": "[Studies on the estimation of the postmortem interval. 9. Synthetic judgment of all sorts of cadaveric findings (author's transl)]. The authors have investigated the relationship between the postmortem interval and all sorts of cadaveric findings, summarizing the results of the previous reports, and have devised a cadaveric-phenomena diagram and a chart with which the postmortem interval can be estimated easily. The validity and utility of the diagram and the chart have been demonstrated by applying them to other autopsy cases than those from which they have been derived."}
{"id": "PMID:468098", "title": "[Chronic hepatitis--clinicopathological studies of 687 cases (author's transl)].", "content": "Chronic hepatitis is one of liver diseases with arguments from the clinical and histopathological aspects. Histopathological examinations were made on 687 biopsy cases clinically diagnosed as chronic hepatitis. Histopathological classification was based on our own criteria by referring to discussions in the series of Inuyama symposia on hepattis and others. The correlation between histological diagnosis and clinical data was also examined. Histopathological diagnoses made of the 687 cases were classified as follows; normal liver or liver with no pathognomonic changes of 77 cases (11.2%), non-specific reactive hepatitis of 56 cases (8.0%), viral hepatitis of 488 cases (71.0%), alcoholic hepatitis of 25 cases (3.6%), fatty liver of 23 cases (3.3%), massive liver necrosis of 3 cases, liver fibrosis of 2 cases, congestive liver of 1 case, and unclassified 12 cases due to inadequate specimens or other reasons. Among 488 viral hepatitis cases, histological stages were as follows; acute hepatitis (38 cases, 7.8%), persistent hepatitis (23 cases, 4.7%), chronic inactive hepatitis (142 cases, 29.1%), chronic active hepatitis (165 cases, 33.8%), chronic hepatitis with subloblar necrosis (33 cases, 6.8%), precirrhosis (51 cases, 10.5%), cirrhosis (27 cases, 5.5%). The relationship between histological aspects and clinical features was discussed by sex, age, and others. Of 41 follow up cases, significant values of histological type, presence of HB ag., or alcoholic were discussed as for the causative factors evolving liver cirrhosis.", "contents": "[Chronic hepatitis--clinicopathological studies of 687 cases (author's transl)]. Chronic hepatitis is one of liver diseases with arguments from the clinical and histopathological aspects. Histopathological examinations were made on 687 biopsy cases clinically diagnosed as chronic hepatitis. Histopathological classification was based on our own criteria by referring to discussions in the series of Inuyama symposia on hepattis and others. The correlation between histological diagnosis and clinical data was also examined. Histopathological diagnoses made of the 687 cases were classified as follows; normal liver or liver with no pathognomonic changes of 77 cases (11.2%), non-specific reactive hepatitis of 56 cases (8.0%), viral hepatitis of 488 cases (71.0%), alcoholic hepatitis of 25 cases (3.6%), fatty liver of 23 cases (3.3%), massive liver necrosis of 3 cases, liver fibrosis of 2 cases, congestive liver of 1 case, and unclassified 12 cases due to inadequate specimens or other reasons. Among 488 viral hepatitis cases, histological stages were as follows; acute hepatitis (38 cases, 7.8%), persistent hepatitis (23 cases, 4.7%), chronic inactive hepatitis (142 cases, 29.1%), chronic active hepatitis (165 cases, 33.8%), chronic hepatitis with subloblar necrosis (33 cases, 6.8%), precirrhosis (51 cases, 10.5%), cirrhosis (27 cases, 5.5%). The relationship between histological aspects and clinical features was discussed by sex, age, and others. Of 41 follow up cases, significant values of histological type, presence of HB ag., or alcoholic were discussed as for the causative factors evolving liver cirrhosis."}
{"id": "PMID:468099", "title": "[Studies on the diagnosis of schistosomiasis japonica. (1) Circumoval precipitin (COP) test as a method of screening of the subjects for fecal examination (author's transl)].", "content": "Examinations were performed on 351 individuals of Shikishima village, Yamanashi Prefecture. Three fecal samples were collected at one-week intervals and examined by MIFC method without iodine. Reactions with a diameter of 9 mm or more in wheal and/or 20 mm or more in erythema were regarded as the positive for the intradermal reaction using Melcher's antigen (1 : 1,000). For the COP test, each one drop of serum and a saline suspension of eggs were mixed on a microscope slide and sealed a coverslip with vaseline. The number of eggs showing precipitates were counted after 48 hrs at 37 degrees C. The results showed that all the precipitates were small in size in the COP titer less than 40%. Medium precipitates appear first in the cases of a 40.% class and the percentage increased thereafter as the COP titer increased. Large precipitate was found in 10% of the eggs affected in a 50% class and found in 40% in the titer of a 80% class. Of 351 tested, a significant higher rate of egg-passers (42.7%, 41/96) was obtained in the COP reactors, as compared with other two groups; 19.4% (41/211) in the dermal reactors, 11.7% (41/351) in the total subjects. Moreover, the egg-passers were recovered in 60--70% of the cases in the COP titer of more than 70%. It was considered that the COP test was a useful diagnostic method for schistosomiasis japonica and the sensitivity and efficiency of this test were superior to those of intradermal test with regarded to the screening of the subjects for fecal examination.", "contents": "[Studies on the diagnosis of schistosomiasis japonica. (1) Circumoval precipitin (COP) test as a method of screening of the subjects for fecal examination (author's transl)]. Examinations were performed on 351 individuals of Shikishima village, Yamanashi Prefecture. Three fecal samples were collected at one-week intervals and examined by MIFC method without iodine. Reactions with a diameter of 9 mm or more in wheal and/or 20 mm or more in erythema were regarded as the positive for the intradermal reaction using Melcher's antigen (1 : 1,000). For the COP test, each one drop of serum and a saline suspension of eggs were mixed on a microscope slide and sealed a coverslip with vaseline. The number of eggs showing precipitates were counted after 48 hrs at 37 degrees C. The results showed that all the precipitates were small in size in the COP titer less than 40%. Medium precipitates appear first in the cases of a 40.% class and the percentage increased thereafter as the COP titer increased. Large precipitate was found in 10% of the eggs affected in a 50% class and found in 40% in the titer of a 80% class. Of 351 tested, a significant higher rate of egg-passers (42.7%, 41/96) was obtained in the COP reactors, as compared with other two groups; 19.4% (41/211) in the dermal reactors, 11.7% (41/351) in the total subjects. Moreover, the egg-passers were recovered in 60--70% of the cases in the COP titer of more than 70%. It was considered that the COP test was a useful diagnostic method for schistosomiasis japonica and the sensitivity and efficiency of this test were superior to those of intradermal test with regarded to the screening of the subjects for fecal examination."}
{"id": "PMID:468100", "title": "[Halothane-pentazocine interaction in dogs (author's transl)].", "content": "It is known that narcotics reduce the alveolar concentration of inhalation anesthetics in man and animals. However the magnitude and duration of narcotic effect on inhalation anesthesia is not known. Accordingly, I determined in dogs the temporal effect of various doses of pentazocine, a commonly used anesthetic adjuvant, on the minimum alveolar concentration (MAC) of halothane. In addition, I compared plasma pentazocine concentrations in dogs both awake and during halothane anesthesia using an identical intramuscular dose of pentazocine. Intramuscular injection of pentazocine, group II (2.5 mg/kg), group III (5 mg/kg), group IV (10 mg/kg) reduced MAC of halothane required for anesthesia. The magnitude of MAC depression were 19.3% of control halothane MAC in group II, 36.4% of control in group III and 41.7% of control in group IV. Postinjection plasma concentration was fitted by computer with a 2-compartment open pharmacokinetic model. Plasma pentazocine concentration for awake (group I) and anesthetized (group II) dogs given the same dose (2.5 mg/kg) did not differ significantly on biological half-life, total apparent volume of distribution and body clearance. Halothane minimum alveolar concentration (MAC) was correlated to plasma pentazocine concentration (r= -0.60) and cerebrospinal fluid pentazocine concentration (r= -0.74).", "contents": "[Halothane-pentazocine interaction in dogs (author's transl)]. It is known that narcotics reduce the alveolar concentration of inhalation anesthetics in man and animals. However the magnitude and duration of narcotic effect on inhalation anesthesia is not known. Accordingly, I determined in dogs the temporal effect of various doses of pentazocine, a commonly used anesthetic adjuvant, on the minimum alveolar concentration (MAC) of halothane. In addition, I compared plasma pentazocine concentrations in dogs both awake and during halothane anesthesia using an identical intramuscular dose of pentazocine. Intramuscular injection of pentazocine, group II (2.5 mg/kg), group III (5 mg/kg), group IV (10 mg/kg) reduced MAC of halothane required for anesthesia. The magnitude of MAC depression were 19.3% of control halothane MAC in group II, 36.4% of control in group III and 41.7% of control in group IV. Postinjection plasma concentration was fitted by computer with a 2-compartment open pharmacokinetic model. Plasma pentazocine concentration for awake (group I) and anesthetized (group II) dogs given the same dose (2.5 mg/kg) did not differ significantly on biological half-life, total apparent volume of distribution and body clearance. Halothane minimum alveolar concentration (MAC) was correlated to plasma pentazocine concentration (r= -0.60) and cerebrospinal fluid pentazocine concentration (r= -0.74)."}
{"id": "PMID:468101", "title": "Sodium sparing effect of alanine in fasting obese females.", "content": "In obese females, 50 g of alanine given orally on the fifth day of fasting, produced a decrease in urinary sodium that persisted the following day. Plasma renin did not increase the day following alanine intake.", "contents": "Sodium sparing effect of alanine in fasting obese females. In obese females, 50 g of alanine given orally on the fifth day of fasting, produced a decrease in urinary sodium that persisted the following day. Plasma renin did not increase the day following alanine intake."}
{"id": "PMID:468102", "title": "Effect of experimental diabetes on the incorporation of amino acids into protein in rat aorta.", "content": "The incorporation of 14C-labelled leucine or phenylalanine into alkali-soluble protein was determined under in vitro conditions in aortic intima-media of normal and streptozotocin-diabetic rats. Two weeks after the induction of diabetes the incorporation of the amino acids into aortic protein was reduced. When determined after diabetes of one week's duration the leucine-14C incorporation was not significantly reduced, while after 5 weeks of diabetes it was severely impaired. After administration of insulin to diabetic rats in vivo for 2 weeks there was no difference in leucine-14C incorporation between normal and diabetic rats. Addition of insulin (0.1 U/ml) in vitro had no effect on the leucine-14C incorporation in either normal or diabetic aorta during incubation times of 3 or 6 h. Elevation of the glucose concentration in vitro from 5.6 to 22.2 mmol/l did not influence the leucine incorporation in diabetic aorta. Both the aortic wet weight and the aortic content of alkali-soluble protein were decreased after 5 weeks of diabetes. The decrease in the protein content of aorta of diabetic animals suggest that the protein synthesis is impaired in vivo.", "contents": "Effect of experimental diabetes on the incorporation of amino acids into protein in rat aorta. The incorporation of 14C-labelled leucine or phenylalanine into alkali-soluble protein was determined under in vitro conditions in aortic intima-media of normal and streptozotocin-diabetic rats. Two weeks after the induction of diabetes the incorporation of the amino acids into aortic protein was reduced. When determined after diabetes of one week's duration the leucine-14C incorporation was not significantly reduced, while after 5 weeks of diabetes it was severely impaired. After administration of insulin to diabetic rats in vivo for 2 weeks there was no difference in leucine-14C incorporation between normal and diabetic rats. Addition of insulin (0.1 U/ml) in vitro had no effect on the leucine-14C incorporation in either normal or diabetic aorta during incubation times of 3 or 6 h. Elevation of the glucose concentration in vitro from 5.6 to 22.2 mmol/l did not influence the leucine incorporation in diabetic aorta. Both the aortic wet weight and the aortic content of alkali-soluble protein were decreased after 5 weeks of diabetes. The decrease in the protein content of aorta of diabetic animals suggest that the protein synthesis is impaired in vivo."}
{"id": "PMID:468103", "title": "Indomethacin fails to alter basal or phenothiazine-induced prolactin concentrations in man.", "content": "In order to evaluate the possible role of prostaglandins in pituitary prolactin (PRL) secretion, PRL was serially measured following perphenazine (Trilafon) ingestion in 8 men before and after 5 days of indomethacin administration. Since estrogens have been shown to modulate prolactin secretion in man, serum steroids including estrone (E1), estradiol (E2), progesterone (P) and testosterone (T) were measured before and after indomethacin ingestion. Serum E1, P and T levels were similar during the pre- and post-indomethacin study periods: 56 +/- 4 (1 SEM) vs 48 +/- 5 pg/ml, 298 +/- 28 vs 315 +/- 32 pg/ml, and 8.1 +/- 0.7 vs 8.6 +/- 0.7 ng/ml, respectively. Serum E2 levels were slightly, but significantly, lower following indomethacin treatment at 30 +/- 3 vs 37 +/- 3 pg/ml (p less than .01). Basal serum PRL concentrations were unaffected by indomethacin administration (9 +/- 3 pre- vs 8 +/- 2 ng/ml post-drug treatment). Integrated perphenazine-induced PRL responses were likewise similar during the 2 study periods: 101 +/- 16 ng . hr/ml during the control period and 104 +/- 14 ng . hr/ml following indomethacin. Thus, short-term indomethacin treatment had no effect on basal or perphenazine-stimulated PRL secretion in men.", "contents": "Indomethacin fails to alter basal or phenothiazine-induced prolactin concentrations in man. In order to evaluate the possible role of prostaglandins in pituitary prolactin (PRL) secretion, PRL was serially measured following perphenazine (Trilafon) ingestion in 8 men before and after 5 days of indomethacin administration. Since estrogens have been shown to modulate prolactin secretion in man, serum steroids including estrone (E1), estradiol (E2), progesterone (P) and testosterone (T) were measured before and after indomethacin ingestion. Serum E1, P and T levels were similar during the pre- and post-indomethacin study periods: 56 +/- 4 (1 SEM) vs 48 +/- 5 pg/ml, 298 +/- 28 vs 315 +/- 32 pg/ml, and 8.1 +/- 0.7 vs 8.6 +/- 0.7 ng/ml, respectively. Serum E2 levels were slightly, but significantly, lower following indomethacin treatment at 30 +/- 3 vs 37 +/- 3 pg/ml (p less than .01). Basal serum PRL concentrations were unaffected by indomethacin administration (9 +/- 3 pre- vs 8 +/- 2 ng/ml post-drug treatment). Integrated perphenazine-induced PRL responses were likewise similar during the 2 study periods: 101 +/- 16 ng . hr/ml during the control period and 104 +/- 14 ng . hr/ml following indomethacin. Thus, short-term indomethacin treatment had no effect on basal or perphenazine-stimulated PRL secretion in men."}
{"id": "PMID:468104", "title": "The blocking effect of magnesium on the secretion of adrenal catecholamines induced by the omission of sodium from the extracellular medium.", "content": "The blocking action of Mg++ on catecholamine release induced by the substitution of extracellular Na+ by an osmotic equivalent amount of sucrose was studied in isolated, perfused bovine adrenal glands. Perfusing glands with 10 mM Mg++ produced at 51.1% inhibition on catecholamine release evoked by Na+ omission. Increasing the concentration of Mg++ to 20 mM this inhibitory effect was enhanced to 90.3%. D-600 (0.3 mM) promoted a marked blockade of acetylcholine-induced release of catechol hormones that was partially and significantly reverted increasing the concentration of Ca++ in the perfusion medium. D-600 (0.3 mM) failed to inhibit the catecholamine-releasing effect of Na+ deprivation. In adrenal glands previously perfused with D-600 (0.3 mM) and then exposed to a Locke solution containing D-600 (0.3 mM) + Mg++ (10 or 20 mM) the inhibition of the secretory responses evoked by the omission of Na+ was of the same magnitude as that obtained when the glands were perfused with Mg++ (10 or 20 mM) in the absence of D-600. These results are compatible with the view that the blocking effect of Mg++ may involve an intracellular site of action and that the access of Mg++ into the chromaffin cell may not be mediated through the Ca++ channels.", "contents": "The blocking effect of magnesium on the secretion of adrenal catecholamines induced by the omission of sodium from the extracellular medium. The blocking action of Mg++ on catecholamine release induced by the substitution of extracellular Na+ by an osmotic equivalent amount of sucrose was studied in isolated, perfused bovine adrenal glands. Perfusing glands with 10 mM Mg++ produced at 51.1% inhibition on catecholamine release evoked by Na+ omission. Increasing the concentration of Mg++ to 20 mM this inhibitory effect was enhanced to 90.3%. D-600 (0.3 mM) promoted a marked blockade of acetylcholine-induced release of catechol hormones that was partially and significantly reverted increasing the concentration of Ca++ in the perfusion medium. D-600 (0.3 mM) failed to inhibit the catecholamine-releasing effect of Na+ deprivation. In adrenal glands previously perfused with D-600 (0.3 mM) and then exposed to a Locke solution containing D-600 (0.3 mM) + Mg++ (10 or 20 mM) the inhibition of the secretory responses evoked by the omission of Na+ was of the same magnitude as that obtained when the glands were perfused with Mg++ (10 or 20 mM) in the absence of D-600. These results are compatible with the view that the blocking effect of Mg++ may involve an intracellular site of action and that the access of Mg++ into the chromaffin cell may not be mediated through the Ca++ channels."}
{"id": "PMID:468106", "title": "Plasma vasopressin variation and renin activity in normal active humans.", "content": "Plasma concentrations of vasopressin and plasma renin activity were measured every 30 min for 24 h in 5 normal active humans, in 1 normal woman confined to bed (except for brief periods up to the bathroom), in 2 active patients with primary aldosteronism and in 1 patient with low-renin hypertension. Plasma vasopressin varied markedly over the day and night in a pattern suggesting episodic secretion of the hormone in the normal subjects. Assumption of upright posture was accompanied by a rise in plasma levels from undetectable to 20--50 pg/ml. Episodic secretion, however, also occurred during bed rest and sleep. In contrast, patients with primary aldosteronism and low-renin hypertension had plasma vasopressin levels considerably lower than the normals, and their profiles of plasma concentration lacked the peaks seen in normals. In the normals, although vasopressin and renin secretion often coincided, only 2 of 6 studies showed a significant correlation between the plasma levels of the two hormones. This study, therefore, shows that vasopressin is secreted periodically in normal humans, that upright posture is an important modulator of secretory activity and that the renin-angiotensin system may or may not influence the pattern of secretion. In addition, it underlines the necessity of recumbency in establishing the existence of a circadian rhythm of plasma vasopressin levels.", "contents": "Plasma vasopressin variation and renin activity in normal active humans. Plasma concentrations of vasopressin and plasma renin activity were measured every 30 min for 24 h in 5 normal active humans, in 1 normal woman confined to bed (except for brief periods up to the bathroom), in 2 active patients with primary aldosteronism and in 1 patient with low-renin hypertension. Plasma vasopressin varied markedly over the day and night in a pattern suggesting episodic secretion of the hormone in the normal subjects. Assumption of upright posture was accompanied by a rise in plasma levels from undetectable to 20--50 pg/ml. Episodic secretion, however, also occurred during bed rest and sleep. In contrast, patients with primary aldosteronism and low-renin hypertension had plasma vasopressin levels considerably lower than the normals, and their profiles of plasma concentration lacked the peaks seen in normals. In the normals, although vasopressin and renin secretion often coincided, only 2 of 6 studies showed a significant correlation between the plasma levels of the two hormones. This study, therefore, shows that vasopressin is secreted periodically in normal humans, that upright posture is an important modulator of secretory activity and that the renin-angiotensin system may or may not influence the pattern of secretion. In addition, it underlines the necessity of recumbency in establishing the existence of a circadian rhythm of plasma vasopressin levels."}
{"id": "PMID:468107", "title": "Comparative study of ethynyloestradiol metabolism in the rabbit, guinea pig and rat.", "content": "Ethynyloestradiol was administered to rabbits, guinea pigs and rats, and the concentration of the steroid in blood was measured by radioimmunoassay. In both rabbits and guinea pigs, levels of conjugated steroid were much higher than those of the freely extractable form. Whereas considerable amounts of steroid were present in a congugated form in plasma 24 h after injection, none was present at this time in a freely extractable form. There were significant differences between young and adult rabbits and guinea pigs in the rate at which ethynyloestradiol was metabolized. The amounts present in the freely extractable form in rats were higher than in the other two species but no steroid was detected in the conjugated fraction. The results are compared with previous findings in humans.", "contents": "Comparative study of ethynyloestradiol metabolism in the rabbit, guinea pig and rat. Ethynyloestradiol was administered to rabbits, guinea pigs and rats, and the concentration of the steroid in blood was measured by radioimmunoassay. In both rabbits and guinea pigs, levels of conjugated steroid were much higher than those of the freely extractable form. Whereas considerable amounts of steroid were present in a congugated form in plasma 24 h after injection, none was present at this time in a freely extractable form. There were significant differences between young and adult rabbits and guinea pigs in the rate at which ethynyloestradiol was metabolized. The amounts present in the freely extractable form in rats were higher than in the other two species but no steroid was detected in the conjugated fraction. The results are compared with previous findings in humans."}
{"id": "PMID:468108", "title": "[Microbial degradation of papaverine (author's transl)].", "content": "A bacterium growing on papaverine as sole carbon and nitrogen source was isolated by incubation of soil with papaverine. The bacterium could be identified as a Nocardia strain by morphological and physiological tests. When growing on papaverine, this strain excretes metabolites into the medium. Based on the structure of the metabolites 1--9 a degradation pathway is proposed. 1 = 1-(3,4-Dimethoxybenzyl)-3,4-dihydro-6,7-dimethoxy-3,4-isoquinolinediol; 2 = 1-(3,4-dimethoxybenzyl)-6,7-dimethoxy-3,4-isoquinolinediol; 3 = 2-(3,4-dimethoxyphenyl)-1-[2-(2-hydroxyethyl)-4,5-dimethoxyphenly]ethanone; 4 = 2-hydroxy-4,5-dimethoxybenzeneethanol; 5 = 3,4-dimethoxybenzeneacetic acid; 6 = 2-hydroxy-4,5-dimethyoxybenzeneacetic acid; 7 = 4-hydroxy-3-methoxybenzeneacetic acid; 8 = 3,4-dimethoxybenzaldehyde; 9 = 2-(hydroxymethyl)-4,5-dimethoxybenzeneethanol.", "contents": "[Microbial degradation of papaverine (author's transl)]. A bacterium growing on papaverine as sole carbon and nitrogen source was isolated by incubation of soil with papaverine. The bacterium could be identified as a Nocardia strain by morphological and physiological tests. When growing on papaverine, this strain excretes metabolites into the medium. Based on the structure of the metabolites 1--9 a degradation pathway is proposed. 1 = 1-(3,4-Dimethoxybenzyl)-3,4-dihydro-6,7-dimethoxy-3,4-isoquinolinediol; 2 = 1-(3,4-dimethoxybenzyl)-6,7-dimethoxy-3,4-isoquinolinediol; 3 = 2-(3,4-dimethoxyphenyl)-1-[2-(2-hydroxyethyl)-4,5-dimethoxyphenly]ethanone; 4 = 2-hydroxy-4,5-dimethoxybenzeneethanol; 5 = 3,4-dimethoxybenzeneacetic acid; 6 = 2-hydroxy-4,5-dimethyoxybenzeneacetic acid; 7 = 4-hydroxy-3-methoxybenzeneacetic acid; 8 = 3,4-dimethoxybenzaldehyde; 9 = 2-(hydroxymethyl)-4,5-dimethoxybenzeneethanol."}
{"id": "PMID:468109", "title": "A novel method for the rapid purification of human and rat fibrin(ogen) degradation products in high yields.", "content": "A novel method is described for the preparation and purification of fibrin(ogen) degradation products in high yields. The high yields are due to two factors: a) an improved preparation method in which the heterogeneity in the size of the degradation products D is greatly reduced by performing the digestion with plasmin at well-controlled calcium concentrations (see ref.[22]). b) a new purification method, which includes Sephadex G-200 filtration and separation of D and E fragments by preparative isoelectric focusing. The latter step gives a complete separation of D and E fragments, without any overlap, and with a nearly 100% recovery in a short period of time. The properties of human and rat fibrin(ogen) degradation products are very similar.", "contents": "A novel method for the rapid purification of human and rat fibrin(ogen) degradation products in high yields. A novel method is described for the preparation and purification of fibrin(ogen) degradation products in high yields. The high yields are due to two factors: a) an improved preparation method in which the heterogeneity in the size of the degradation products D is greatly reduced by performing the digestion with plasmin at well-controlled calcium concentrations (see ref.[22]). b) a new purification method, which includes Sephadex G-200 filtration and separation of D and E fragments by preparative isoelectric focusing. The latter step gives a complete separation of D and E fragments, without any overlap, and with a nearly 100% recovery in a short period of time. The properties of human and rat fibrin(ogen) degradation products are very similar."}
{"id": "PMID:468110", "title": "Hemocyanins in spiders, VI[1]. Comparison of the polypeptide chains of Eurypelma californicum hemocyanin.", "content": "The subunits of the hemocyanin from the tarantula, Eurypelma californicum, were isolated, following dissociation at pH 9.6, by a sequence of chromatographic and electrophoretic steps. Fraction 2 (containing two chains, a and c2) and the constituent polypeptide chains of the dimeric subunit 4D (b and c4) were resolved by anion exchange chromatography at pH 8.9 and 6.5, respectively. Since c2 and c4 have different electrophoretic mobilities in polyacrylamide gradient gels, the total number of different polypeptide chains is seven. The amino acid compositions of the seven chains are reported. There are major differences for at least half of the amino acids, while more consistent proportions become evident, if the amino acids are grouped by types of side chains. The N-terminal amino acid is proline in the case of chains b and e,, while no end group called be detected in any of the other chains by different methods. The C-terminal end group was found to be valine in both chains d and e. Cleavage by 70% formic acid, and by cyanogen bromide in formic acid results in fragmentation patterns distinct for each chain. After cyanogen bromide cleavage, the two largest peptides of each chain are of molecular weight near 2400. Tryptic fingerprints also reveal significant differences between all chains. Subunit heterogeneity of Eurypelma hemocyanin is clearly not the consequence of secondary modifications, but resides in major differences of the amino acid sequences.", "contents": "Hemocyanins in spiders, VI[1]. Comparison of the polypeptide chains of Eurypelma californicum hemocyanin. The subunits of the hemocyanin from the tarantula, Eurypelma californicum, were isolated, following dissociation at pH 9.6, by a sequence of chromatographic and electrophoretic steps. Fraction 2 (containing two chains, a and c2) and the constituent polypeptide chains of the dimeric subunit 4D (b and c4) were resolved by anion exchange chromatography at pH 8.9 and 6.5, respectively. Since c2 and c4 have different electrophoretic mobilities in polyacrylamide gradient gels, the total number of different polypeptide chains is seven. The amino acid compositions of the seven chains are reported. There are major differences for at least half of the amino acids, while more consistent proportions become evident, if the amino acids are grouped by types of side chains. The N-terminal amino acid is proline in the case of chains b and e,, while no end group called be detected in any of the other chains by different methods. The C-terminal end group was found to be valine in both chains d and e. Cleavage by 70% formic acid, and by cyanogen bromide in formic acid results in fragmentation patterns distinct for each chain. After cyanogen bromide cleavage, the two largest peptides of each chain are of molecular weight near 2400. Tryptic fingerprints also reveal significant differences between all chains. Subunit heterogeneity of Eurypelma hemocyanin is clearly not the consequence of secondary modifications, but resides in major differences of the amino acid sequences."}
{"id": "PMID:468111", "title": "Purification and N-terminal amino acid sequence determination of anionic and cationic canine trypsinogens.", "content": "Anionic and cationic canine trypsinogens were purified from pancreatic juice by affinity chromatography with Trasylol coupled to Sepharose 4B followed by ion exchange chromatography with SP-Sephadex C-50. Automatic N-terminal amino acid sequence determination showed the following structures for the activation peptides: Thr-Pro-Thr-Asp-Asp-Asp-Asp-Lys for anionic trypsinogen, and Phe-Pro-Ile-Asp-Asp-Asp-Asp-Lys for cationic trypsinogen.", "contents": "Purification and N-terminal amino acid sequence determination of anionic and cationic canine trypsinogens. Anionic and cationic canine trypsinogens were purified from pancreatic juice by affinity chromatography with Trasylol coupled to Sepharose 4B followed by ion exchange chromatography with SP-Sephadex C-50. Automatic N-terminal amino acid sequence determination showed the following structures for the activation peptides: Thr-Pro-Thr-Asp-Asp-Asp-Asp-Lys for anionic trypsinogen, and Phe-Pro-Ile-Asp-Asp-Asp-Asp-Lys for cationic trypsinogen."}
{"id": "PMID:468112", "title": "[The primary structure of the hemoglobin of the greylag goose (Answer anser) and the unequal evolution of the beta-chains (an experimental approach to a biochemical analysis of behaviour (author's transl)].", "content": "The primary structures of the alpha- and beta-chains from greylag goose (Anser anser) hemoglobin are given. The sequence was deduced automatically in the sequenator. They differ from chicken alpha-chains in the exchange of 30, from beta-chains in the exchange of only 8 amino acid residues, respectively. The contact points of inositol pentaphosphate with the beta-chains are identical in chicken and greylag goose. Unequal evolution of the beta-chains was found, which is published here for the first time. By comparing the sequences of chicken and greylag goose and considering paleontological data, we found the mutation rate of the alpha-chains to be normal, i.e. 6 million years/mutation. This corresponds to the values for other species. The mutation rate of beta-chains is reduced and was calculated at 25 million years/mutation. This is possibly due to a specific function of beta-chains. This paper is the basis of our attempt to explain on a molecular basis the ability of bar-headed goose (Anser indicus) to fly and breathe at high altitudes.", "contents": "[The primary structure of the hemoglobin of the greylag goose (Answer anser) and the unequal evolution of the beta-chains (an experimental approach to a biochemical analysis of behaviour (author's transl)]. The primary structures of the alpha- and beta-chains from greylag goose (Anser anser) hemoglobin are given. The sequence was deduced automatically in the sequenator. They differ from chicken alpha-chains in the exchange of 30, from beta-chains in the exchange of only 8 amino acid residues, respectively. The contact points of inositol pentaphosphate with the beta-chains are identical in chicken and greylag goose. Unequal evolution of the beta-chains was found, which is published here for the first time. By comparing the sequences of chicken and greylag goose and considering paleontological data, we found the mutation rate of the alpha-chains to be normal, i.e. 6 million years/mutation. This corresponds to the values for other species. The mutation rate of beta-chains is reduced and was calculated at 25 million years/mutation. This is possibly due to a specific function of beta-chains. This paper is the basis of our attempt to explain on a molecular basis the ability of bar-headed goose (Anser indicus) to fly and breathe at high altitudes."}
{"id": "PMID:468113", "title": "Hepatic membrane proteins involved in ribosome binding: identification by three procedures.", "content": "Rat liver ribosomes, isolated from rough-surfaced endoplasmic reticulum using non-ionic detergent in the presence of 25 mM KCl, were associated with non-ribosomal proteins, presumably of membranous origin. These proteins could be isolated by extracting such ribosome fractions with either deoxycholate or non-ionic detergents at higher concentrations of KCl. Analysis of the extracts by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate revealed the presence of a number of discrete polypeptides having the following approximate molecular weights: 166,000, 107,000, 100,000, 65,000 and 36,000. Ribosomes associated with the membrane-derived proteins reattached to degranulated membranes in vitro less well than did ribosomes prepared in ways which removed the proteins. Extraction of a set of similar proteins from degranulated endoplasmic reticulum by treatment with buffered 1 M urea, also interfered with ribosome reattachment. A third approach to the identification of proteins associated with ribosome attachment sites involved the labelling with radioactive succinic anhydride of apparently similar proteins in degranulated membranes, after prior treatment of the latter, before removal of bound ribosomes, with unlabelled reagent. The results indicate that certain membrane proteins may be part of the receptor sites for binding of ribosomes to the endoplasmic reticulum in rat liver.", "contents": "Hepatic membrane proteins involved in ribosome binding: identification by three procedures. Rat liver ribosomes, isolated from rough-surfaced endoplasmic reticulum using non-ionic detergent in the presence of 25 mM KCl, were associated with non-ribosomal proteins, presumably of membranous origin. These proteins could be isolated by extracting such ribosome fractions with either deoxycholate or non-ionic detergents at higher concentrations of KCl. Analysis of the extracts by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate revealed the presence of a number of discrete polypeptides having the following approximate molecular weights: 166,000, 107,000, 100,000, 65,000 and 36,000. Ribosomes associated with the membrane-derived proteins reattached to degranulated membranes in vitro less well than did ribosomes prepared in ways which removed the proteins. Extraction of a set of similar proteins from degranulated endoplasmic reticulum by treatment with buffered 1 M urea, also interfered with ribosome reattachment. A third approach to the identification of proteins associated with ribosome attachment sites involved the labelling with radioactive succinic anhydride of apparently similar proteins in degranulated membranes, after prior treatment of the latter, before removal of bound ribosomes, with unlabelled reagent. The results indicate that certain membrane proteins may be part of the receptor sites for binding of ribosomes to the endoplasmic reticulum in rat liver."}
{"id": "PMID:468114", "title": "[Synthesis of the human proinsulin sequence 71-86, I: Synthesis of sequence 71-86 as a monomeric cyclic biscystine peptide derivative and as its tetra-S-trityl derivative (author's transl)].", "content": "The synthesis of sequence 71-86 of human proinsulin (sequence 6-21 of human insulin A-chain) via its monomeric cyclic dicystine peptide derivative (XXII) and the corresponding tetra-S-trityl-peptide derivative (XVIII) is described. The good solubility of the derivatives (XXII, XVIII) in orgranic solvents makes it possible to purify both the derivatives in their protected form. The S-trityl derivative (XVIII) enables the synthesis of the fragment 71-86 in a scale needed for the planned synthesis of human proinsulin.", "contents": "[Synthesis of the human proinsulin sequence 71-86, I: Synthesis of sequence 71-86 as a monomeric cyclic biscystine peptide derivative and as its tetra-S-trityl derivative (author's transl)]. The synthesis of sequence 71-86 of human proinsulin (sequence 6-21 of human insulin A-chain) via its monomeric cyclic dicystine peptide derivative (XXII) and the corresponding tetra-S-trityl-peptide derivative (XVIII) is described. The good solubility of the derivatives (XXII, XVIII) in orgranic solvents makes it possible to purify both the derivatives in their protected form. The S-trityl derivative (XVIII) enables the synthesis of the fragment 71-86 in a scale needed for the planned synthesis of human proinsulin."}
{"id": "PMID:468115", "title": "Synthesis of human proinsulin sequence 71-86, II: Improved synthesis of the fragment.", "content": "An improved synthesis of a fully protected hexadecapeptide Bpoc-Cys(Trt)-Cys-(Trt)-Thr(But)-Ser(But)-Ile-Cys(Trt)-Ser(But)-Leu-Tyr(But)-Gln-Leu-Glu(OBut)-Asn-Tyr(But)-Cys(Trt)-Asn-OBut, which corresponds to the amino acid sequence 71-86 of human proinsulin, is described. The final product was obtained by the condensation of three fragments 71-74, 75-77 and 78-86.", "contents": "Synthesis of human proinsulin sequence 71-86, II: Improved synthesis of the fragment. An improved synthesis of a fully protected hexadecapeptide Bpoc-Cys(Trt)-Cys-(Trt)-Thr(But)-Ser(But)-Ile-Cys(Trt)-Ser(But)-Leu-Tyr(But)-Gln-Leu-Glu(OBut)-Asn-Tyr(But)-Cys(Trt)-Asn-OBut, which corresponds to the amino acid sequence 71-86 of human proinsulin, is described. The final product was obtained by the condensation of three fragments 71-74, 75-77 and 78-86."}
{"id": "PMID:468116", "title": "[Synthesis of the human proinsulin sequence 71-86, III: Synthesis via the fragments 71-78 and 79-86 (author's transl)].", "content": "The synthesis of the sequence 71-86 (XIII) of human proinsulin (sequence 6-21 of human insulin-A-chain) via the fragments 71-78 (XIy1,3) and 79-86 (XII) is described. The good solubility of the protected peptide derivatives belonging to the sequences 75-78 (IXx1,2), 79-86 (X), and 71-78 (XIy1,2), and of the fragment 71-86 (XIII) itself in organic solvents allows a quick and efficient purification of these derivatives by liquid chromatography on silica-gel columns.", "contents": "[Synthesis of the human proinsulin sequence 71-86, III: Synthesis via the fragments 71-78 and 79-86 (author's transl)]. The synthesis of the sequence 71-86 (XIII) of human proinsulin (sequence 6-21 of human insulin-A-chain) via the fragments 71-78 (XIy1,3) and 79-86 (XII) is described. The good solubility of the protected peptide derivatives belonging to the sequences 75-78 (IXx1,2), 79-86 (X), and 71-78 (XIy1,2), and of the fragment 71-86 (XIII) itself in organic solvents allows a quick and efficient purification of these derivatives by liquid chromatography on silica-gel columns."}
{"id": "PMID:468117", "title": "Localization of the charge differences in the actins of rabbit skeletal muscle and chicken gizzard by two-dimensional gel electrophoretic analysis of tryptic fragments.", "content": "Partial tryptic cleavage products of pure actin from rabbit skeletal muscle and chicken gizzard are compared by two-dimensional electrophoresis in polyacrylamide gels with respect to isoelectric point and molecular weight. While the intact polypeptides (Mr 42,000) have different isoelectric points, two large cleavage products (Mr 35,000) generated from both both actin species have identical isoelectric points and identical molecular weights. These relatively trypsin-resistant cleavage products are presumably identical to the known \"core actin\" fragments which lack the aminoterminal region of the polypeptide chain. Therefore the differences that are responsible for the different isoelectric points of rabbit skeletal muscle actin and chicken gizzard actin seem to be restricted to the aminoterminal part of the actin polypeptide chains as was proposed on the basis of partial amino acid sequence data.", "contents": "Localization of the charge differences in the actins of rabbit skeletal muscle and chicken gizzard by two-dimensional gel electrophoretic analysis of tryptic fragments. Partial tryptic cleavage products of pure actin from rabbit skeletal muscle and chicken gizzard are compared by two-dimensional electrophoresis in polyacrylamide gels with respect to isoelectric point and molecular weight. While the intact polypeptides (Mr 42,000) have different isoelectric points, two large cleavage products (Mr 35,000) generated from both both actin species have identical isoelectric points and identical molecular weights. These relatively trypsin-resistant cleavage products are presumably identical to the known \"core actin\" fragments which lack the aminoterminal region of the polypeptide chain. Therefore the differences that are responsible for the different isoelectric points of rabbit skeletal muscle actin and chicken gizzard actin seem to be restricted to the aminoterminal part of the actin polypeptide chains as was proposed on the basis of partial amino acid sequence data."}
{"id": "PMID:468119", "title": "[Synthesis of tyrosin O-sulfate-containing peptides (author's transl)].", "content": "A new synthetic route to tyrosine-O-sulfate-containing peptides based on the direct use of N alpha-acyltyrosine-O-sulfate is described and exemplified by the synthesis of the [Thr28,Leu31]cholecystokinin-pankreozymin-(25-33)-nonapeptide.", "contents": "[Synthesis of tyrosin O-sulfate-containing peptides (author's transl)]. A new synthetic route to tyrosine-O-sulfate-containing peptides based on the direct use of N alpha-acyltyrosine-O-sulfate is described and exemplified by the synthesis of the [Thr28,Leu31]cholecystokinin-pankreozymin-(25-33)-nonapeptide."}
{"id": "PMID:468120", "title": "M\u00fcllerian adenosarcoma of the uterine body: a report of nine cases.", "content": "Nine cases of Mullerian adenosarcoma of the uterine body, first defined in 1974 by Clement & Scully, are described. The tumour usually occurs in elderly women and appears macroscopically as a polypoid mass filling the uterine cavity. Histologically these neoplasms are characterized by having a benign epithelial component set in a sarcomatous stroma. The epithelial element consists of tubular glands, cystically dilated glands and a surface epithelium. The glandular and surface epithelium usually resembles that of proliferative endometrium but may be of mucinous type and can undergo squamous metaplasia. Occasional mitotic figures, multilayering and intraluminal tufting are seen in the actively growing but benign glands which are considered to be an integral component of the tumour. The sarcomatous stroma contains spindle cells, round cells and, less frequently, multinucleated giant cells and rhabdomyoblasts. The Mullerian adenosarcoma represents a relatively benign variant of the mixed mesenchymal sarcoma of the uterus and is probably more common than is generally realized. Myometrial invasion is rarely deep and the majority of patients with this neoplasm survive. Vaginal recurrence occurs only in a minority of patients and distant metastasis is exceptional.", "contents": "M\u00fcllerian adenosarcoma of the uterine body: a report of nine cases. Nine cases of Mullerian adenosarcoma of the uterine body, first defined in 1974 by Clement & Scully, are described. The tumour usually occurs in elderly women and appears macroscopically as a polypoid mass filling the uterine cavity. Histologically these neoplasms are characterized by having a benign epithelial component set in a sarcomatous stroma. The epithelial element consists of tubular glands, cystically dilated glands and a surface epithelium. The glandular and surface epithelium usually resembles that of proliferative endometrium but may be of mucinous type and can undergo squamous metaplasia. Occasional mitotic figures, multilayering and intraluminal tufting are seen in the actively growing but benign glands which are considered to be an integral component of the tumour. The sarcomatous stroma contains spindle cells, round cells and, less frequently, multinucleated giant cells and rhabdomyoblasts. The Mullerian adenosarcoma represents a relatively benign variant of the mixed mesenchymal sarcoma of the uterus and is probably more common than is generally realized. Myometrial invasion is rarely deep and the majority of patients with this neoplasm survive. Vaginal recurrence occurs only in a minority of patients and distant metastasis is exceptional."}
{"id": "PMID:468121", "title": "Morphological findings in idiopathic calcification of the ascending aorta and aortic valve affecting a young woman.", "content": "The pathology of a case of idiopathic calcification affecting the ascending aorta in a young woman is presented. A varying width of media throughout the aorta and extending into its proximaques of calcium, found in the acellular media, were confined to the ascending aorta. No inflammatory or reparative reaction was seen in the vessel wall. Electron microscopically, the calcium seemed to have an affinity for elastic tissue elements of all sizes and the mode of deposition appeared to be by 'avenues' of the microfibrillar component. Possible pathogenetic mechanisms are discussed.", "contents": "Morphological findings in idiopathic calcification of the ascending aorta and aortic valve affecting a young woman. The pathology of a case of idiopathic calcification affecting the ascending aorta in a young woman is presented. A varying width of media throughout the aorta and extending into its proximaques of calcium, found in the acellular media, were confined to the ascending aorta. No inflammatory or reparative reaction was seen in the vessel wall. Electron microscopically, the calcium seemed to have an affinity for elastic tissue elements of all sizes and the mode of deposition appeared to be by 'avenues' of the microfibrillar component. Possible pathogenetic mechanisms are discussed."}
{"id": "PMID:468122", "title": "A variant of intestinal metaplasia associated with gastric carcinoma: a histochemical study.", "content": "Gastrectomy specimens including 32 cases of carcinoma and 35 cases of peptic ulcer were examined in detail histologically and histochemically to show the distribution of intestinal metaplasia (IM) and types of mucin secreted. A variant of IM, with extensive involvement of both antrum and body and excessive secretion of sulphomucin, was found to be associated with carcinomas showing appreciable extracellular sulphomucin secretion. The identification of such a variant of IM may help the interpretation of gastric biopsies.", "contents": "A variant of intestinal metaplasia associated with gastric carcinoma: a histochemical study. Gastrectomy specimens including 32 cases of carcinoma and 35 cases of peptic ulcer were examined in detail histologically and histochemically to show the distribution of intestinal metaplasia (IM) and types of mucin secreted. A variant of IM, with extensive involvement of both antrum and body and excessive secretion of sulphomucin, was found to be associated with carcinomas showing appreciable extracellular sulphomucin secretion. The identification of such a variant of IM may help the interpretation of gastric biopsies."}
{"id": "PMID:468123", "title": "Temporal (granulomatous) arteritis: a histopathological study of 32 cases.", "content": "Thirty-two cases of temporal arteritis were reviewed. All patients were adults with a mean age of 69.6 years, and no sex predominance. The erythrocyte sedimentation rate was raised and there was a good response to steroid therapy in the 29 patients who were adequately documented and followed up. Detailed histopathological study of the temporal artery biopsies showed three main histopathological variants; 12 cases (37.5%) had predominantly intimal proliferative changes, four cases (12.5%) had granulomatous inflammation without giant cells and 16 (50%) had granulomatous inflammation with giant cells. The predominantly intimal change variant, consisting of a cellular proliferation of fibroblasts and myointimal cells with little or no changes in the media represent an active pathological process and not a healed disease as has been previously suggested. The internal elastic lamina showed abnormal features in all three morphological variants. The need to recognize this variant, the temporal relationship between these variants and the need to study multiple sections are discussed. Biopsy of clinically normal temporal arteries in patients suspected of having temporal arteritis is recommended as some of these vessels may show disease. No correlation was observed between the clinical picture and the histopathological findings.", "contents": "Temporal (granulomatous) arteritis: a histopathological study of 32 cases. Thirty-two cases of temporal arteritis were reviewed. All patients were adults with a mean age of 69.6 years, and no sex predominance. The erythrocyte sedimentation rate was raised and there was a good response to steroid therapy in the 29 patients who were adequately documented and followed up. Detailed histopathological study of the temporal artery biopsies showed three main histopathological variants; 12 cases (37.5%) had predominantly intimal proliferative changes, four cases (12.5%) had granulomatous inflammation without giant cells and 16 (50%) had granulomatous inflammation with giant cells. The predominantly intimal change variant, consisting of a cellular proliferation of fibroblasts and myointimal cells with little or no changes in the media represent an active pathological process and not a healed disease as has been previously suggested. The internal elastic lamina showed abnormal features in all three morphological variants. The need to recognize this variant, the temporal relationship between these variants and the need to study multiple sections are discussed. Biopsy of clinically normal temporal arteries in patients suspected of having temporal arteritis is recommended as some of these vessels may show disease. No correlation was observed between the clinical picture and the histopathological findings."}
{"id": "PMID:468124", "title": "The pathology of a cricopharyngeal dysphagia.", "content": "The paper describes the changes in the cricopharyngeal muscle in seven cases of dysphagia resulting from obstruction at this level which was relieved by myotomy. Histological features included degeneration and regeneration in the muscle fibres with interstitial fibrosis which was severe in some of the cases. It is considered that this restrictive fibrosis is the cause of the dysphagia and that it is secondary to muscle fibre damage, the cause of which is at present obscure. Minor degrees of muscle damage and regeneration were seen rarely in controls and fibrosis was never present. There was no evidence of underlying vascular or neurological disease in six cases; the seventh had a previous history of scleroderma but this was not thought to be the cause of the cricopharyngeal lesion. The age incidence ranged from 1 to 5 years at the onset of dysphagia; in six of the seven it was 50 years or more.", "contents": "The pathology of a cricopharyngeal dysphagia. The paper describes the changes in the cricopharyngeal muscle in seven cases of dysphagia resulting from obstruction at this level which was relieved by myotomy. Histological features included degeneration and regeneration in the muscle fibres with interstitial fibrosis which was severe in some of the cases. It is considered that this restrictive fibrosis is the cause of the dysphagia and that it is secondary to muscle fibre damage, the cause of which is at present obscure. Minor degrees of muscle damage and regeneration were seen rarely in controls and fibrosis was never present. There was no evidence of underlying vascular or neurological disease in six cases; the seventh had a previous history of scleroderma but this was not thought to be the cause of the cricopharyngeal lesion. The age incidence ranged from 1 to 5 years at the onset of dysphagia; in six of the seven it was 50 years or more."}
{"id": "PMID:468125", "title": "Case report: ultrastructure of mature neurogenic implants from ovarian immature teratoma.", "content": "An ultrastructural study of mature (grade O) neurogenic peritoneal implants from an ovarian immature teratoma grade I is presented. The presence of different types of neuroectodermal cells challenges the current terminology of this lesion, which implies a single proliferating cell type. The ultrastructural features were indicative of maturity in all cell lines and even the neurons exhibited well formed synapses. Marked cytoplasmic fibrillary change, similar to that found in central nervous system gliosis, was observed in the astrocytes. The ependymal cells had abnormal cilia with an altered configuration and number of microtubules.", "contents": "Case report: ultrastructure of mature neurogenic implants from ovarian immature teratoma. An ultrastructural study of mature (grade O) neurogenic peritoneal implants from an ovarian immature teratoma grade I is presented. The presence of different types of neuroectodermal cells challenges the current terminology of this lesion, which implies a single proliferating cell type. The ultrastructural features were indicative of maturity in all cell lines and even the neurons exhibited well formed synapses. Marked cytoplasmic fibrillary change, similar to that found in central nervous system gliosis, was observed in the astrocytes. The ependymal cells had abnormal cilia with an altered configuration and number of microtubules."}
{"id": "PMID:468126", "title": "The non-specific nature of the myocardial wavy fibre.", "content": "Wavy myocardial fibres were found in about half each of a series of 28 normal and 31 infarcted human hearts, as well as in the normal heart of an infant. Such fibres were also seen in rather more than half of a series of normal rat hearts. Thus, the wavy fibre is not a specific feature of acute ischaemic heart disease. Some experimental evidence was obtained that patchy loss or preservation of ATP promotes the formation of wavy fibres.", "contents": "The non-specific nature of the myocardial wavy fibre. Wavy myocardial fibres were found in about half each of a series of 28 normal and 31 infarcted human hearts, as well as in the normal heart of an infant. Such fibres were also seen in rather more than half of a series of normal rat hearts. Thus, the wavy fibre is not a specific feature of acute ischaemic heart disease. Some experimental evidence was obtained that patchy loss or preservation of ATP promotes the formation of wavy fibres."}
{"id": "PMID:468129", "title": "Sudden unexpected cardiac death--a practical approach to the forensic problem.", "content": "Sudden cardiac death can usually be resolved by the pathologist into ischaemic heart disease, non-vascular cardiac disease such as aortic stenosis or hypertrophic obstructive cardiomyopathy and infrequently a morphologically normal heart on naked eye examination. When ischaemic heart disease is present one third of cases have a recent occlusive coronary artery thrombosis. Two thirds of patients have coronary stenosis only; the minimum degree of disease reasonably associated with sudden death is one area of 85% stenosis. The majority of patients, however, have multiple areas of stenosis. The predominant causes of non-ischaemic sudden death are severe LV hypertrophy, hypertrophic obstructive cardiomyopathy and the prolapsing mitral valve syndrome. Where the heart and coronary arteries are morphologically normal, review of any previous ECG's, a family history and histological examination of the myocardium and conduction system may reveal a cause or at least allow a reasonable assumption of cardiac arrhythmia to be made. Sudden unexpected death where the circumstances strongly suggest a cardiac cause may pose problems for the pathologist. Ischaemic heart disease (coronary atherosclerosis) is undoubtedly the most frequent cause but even when this is so the detailed pathology is controversial. It is when coronary artery disease is conspicuously absent, often in young individuals previously in good health, that a problem exists. Sudden death in infancy (cot death) is a different entity with its own problems and is not here discussed further.", "contents": "Sudden unexpected cardiac death--a practical approach to the forensic problem. Sudden cardiac death can usually be resolved by the pathologist into ischaemic heart disease, non-vascular cardiac disease such as aortic stenosis or hypertrophic obstructive cardiomyopathy and infrequently a morphologically normal heart on naked eye examination. When ischaemic heart disease is present one third of cases have a recent occlusive coronary artery thrombosis. Two thirds of patients have coronary stenosis only; the minimum degree of disease reasonably associated with sudden death is one area of 85% stenosis. The majority of patients, however, have multiple areas of stenosis. The predominant causes of non-ischaemic sudden death are severe LV hypertrophy, hypertrophic obstructive cardiomyopathy and the prolapsing mitral valve syndrome. Where the heart and coronary arteries are morphologically normal, review of any previous ECG's, a family history and histological examination of the myocardium and conduction system may reveal a cause or at least allow a reasonable assumption of cardiac arrhythmia to be made. Sudden unexpected death where the circumstances strongly suggest a cardiac cause may pose problems for the pathologist. Ischaemic heart disease (coronary atherosclerosis) is undoubtedly the most frequent cause but even when this is so the detailed pathology is controversial. It is when coronary artery disease is conspicuously absent, often in young individuals previously in good health, that a problem exists. Sudden death in infancy (cot death) is a different entity with its own problems and is not here discussed further."}
{"id": "PMID:468130", "title": "New findings of a lamina densa in relation to plasma cells.", "content": "In an ultrastructural study of oral lesions in lichen planus, it was found that some plasma cells have an associated lamina densa. Subsequently, similar appearances have been found in pemphigus vulgaris, in the inflammatory cell infiltrates in dental granulomas and cysts and in a patient with Ehlers-Danlos syndrome. The lamina densa was mainly present adjacent to those parts of the membrane of the plasma cell that were invested with fibrillar connective tissue. The lamina was similar in thickness and electron density to that surrounding Schwann cells and endothelial cells. It is believed that lamina densa has not previously been reported in relation to plasma cells. It is of interest that in all other situations in which lamina densa has been described, the cells are considered to be part of the normal cell population of the tissue, whereas plasma cells are not regarded as part of the resident population of connective tissue.", "contents": "New findings of a lamina densa in relation to plasma cells. In an ultrastructural study of oral lesions in lichen planus, it was found that some plasma cells have an associated lamina densa. Subsequently, similar appearances have been found in pemphigus vulgaris, in the inflammatory cell infiltrates in dental granulomas and cysts and in a patient with Ehlers-Danlos syndrome. The lamina densa was mainly present adjacent to those parts of the membrane of the plasma cell that were invested with fibrillar connective tissue. The lamina was similar in thickness and electron density to that surrounding Schwann cells and endothelial cells. It is believed that lamina densa has not previously been reported in relation to plasma cells. It is of interest that in all other situations in which lamina densa has been described, the cells are considered to be part of the normal cell population of the tissue, whereas plasma cells are not regarded as part of the resident population of connective tissue."}
{"id": "PMID:468131", "title": "Hodgkin's disease with lymphocytic predominance, nodular type (nodular paragranuloma) and progressively transformed germinal centres--a cytohistological study.", "content": "The histology, cytology, and enzyme cytochemistry of a nodular variant of Hodgkin's disease with lymphocytic predominance, called 'nodular paragranuloma', are presented. The histological features of nodular paragranuloma are compared with those of progressively transformed germinal centres, which are enlarged follicles showing a predominance of small lymphocytes and some residual germinal centre cells. Progressively transformed germinal centres are sometimes found in nonspecific lymphadenitis (reactive hyperplasia). The histological similarity and the association between lymph nodes with nodular paragranuloma and lymph nodes with progressively transformed germinal centres in the same patient at different moments or at the same time, suggest that progressively transformed germinal centres are the origin of nodular paragranuloma. Hence, it must be concluded that nodular paragranuloma takes place in B-cell areas of the lymph node, unlike the other, or at least most of the other, types of Hodgkin's disease.", "contents": "Hodgkin's disease with lymphocytic predominance, nodular type (nodular paragranuloma) and progressively transformed germinal centres--a cytohistological study. The histology, cytology, and enzyme cytochemistry of a nodular variant of Hodgkin's disease with lymphocytic predominance, called 'nodular paragranuloma', are presented. The histological features of nodular paragranuloma are compared with those of progressively transformed germinal centres, which are enlarged follicles showing a predominance of small lymphocytes and some residual germinal centre cells. Progressively transformed germinal centres are sometimes found in nonspecific lymphadenitis (reactive hyperplasia). The histological similarity and the association between lymph nodes with nodular paragranuloma and lymph nodes with progressively transformed germinal centres in the same patient at different moments or at the same time, suggest that progressively transformed germinal centres are the origin of nodular paragranuloma. Hence, it must be concluded that nodular paragranuloma takes place in B-cell areas of the lymph node, unlike the other, or at least most of the other, types of Hodgkin's disease."}
{"id": "PMID:468132", "title": "Benign and malignant lymphoid lesions of the stomach. A histological reappraisal in the light of the Kiel classification for non-Hodgkin's lymphomas.", "content": "Twenty-two cases of lymphoid tumours of the stomach were reviewed by application of the Kiel classification for non-Hodgkin's malignant lymphomas. Four cases of pseudolymphomas were found, one of which had been previously misdiagnosed as malignant lymphoma. The remaining cases were all malignant tumours with B-cell lymphoma features. These were divided into seven low grade lymphomas (three immunocytomas and four centroblastic/centrocytic) and II high grade lymphomas (six centroblastic and five immunoblastic lymphomas). No cases of Hodgkin's disease or lymphoblastic lymphoma were observed. The Kiel nomenclature was not only easy to apply, but also helped to differentiate pseudolymphoma from malignant lymphoma. Both the pseudolymphomas and the malignant lymphomas were consistently associated with follicular gastritis. This lesion, while intrinsically non specific, was sometimes accompanied by suggestions of transition between itself and the lymphoma, a fact which at least raises the possibility of a transformation of the former into the latter.", "contents": "Benign and malignant lymphoid lesions of the stomach. A histological reappraisal in the light of the Kiel classification for non-Hodgkin's lymphomas. Twenty-two cases of lymphoid tumours of the stomach were reviewed by application of the Kiel classification for non-Hodgkin's malignant lymphomas. Four cases of pseudolymphomas were found, one of which had been previously misdiagnosed as malignant lymphoma. The remaining cases were all malignant tumours with B-cell lymphoma features. These were divided into seven low grade lymphomas (three immunocytomas and four centroblastic/centrocytic) and II high grade lymphomas (six centroblastic and five immunoblastic lymphomas). No cases of Hodgkin's disease or lymphoblastic lymphoma were observed. The Kiel nomenclature was not only easy to apply, but also helped to differentiate pseudolymphoma from malignant lymphoma. Both the pseudolymphomas and the malignant lymphomas were consistently associated with follicular gastritis. This lesion, while intrinsically non specific, was sometimes accompanied by suggestions of transition between itself and the lymphoma, a fact which at least raises the possibility of a transformation of the former into the latter."}
{"id": "PMID:468133", "title": "The trachea in children with tracheo-oesophageal fistula.", "content": "Forty tracheas from children with tracheo-oesophageal fistulae were histologically analysed for structural deformity. Thirty (75%) had a deficiency of cartilage; 24 (60%) showed an increase in the length of the transverse muscle, and 26 (65%) had a longer than average internal perimeter. Only six tracheas were entirely normal. The position of the fistula bore little relationship to the abnormalities found. Since in only nine patients were abnormalities restricted to the site of the fistula, we believe that careful examination of the whole length of the trachea is needed at the time of surgery to determine the extent of the anomaly and to anticipate tracheal problems. This is also relevant to autopsy studies and to elucidate the cause of death.", "contents": "The trachea in children with tracheo-oesophageal fistula. Forty tracheas from children with tracheo-oesophageal fistulae were histologically analysed for structural deformity. Thirty (75%) had a deficiency of cartilage; 24 (60%) showed an increase in the length of the transverse muscle, and 26 (65%) had a longer than average internal perimeter. Only six tracheas were entirely normal. The position of the fistula bore little relationship to the abnormalities found. Since in only nine patients were abnormalities restricted to the site of the fistula, we believe that careful examination of the whole length of the trachea is needed at the time of surgery to determine the extent of the anomaly and to anticipate tracheal problems. This is also relevant to autopsy studies and to elucidate the cause of death."}
{"id": "PMID:468134", "title": "Foetal remnants in the uterus and their relation to other uterine heterotopia.", "content": "Foetal remnants were found in uterine curettings obtained from three women complaining of irregular vaginal bleeding. These findings are discussed in relation to other cases in the literature in which heterotopic tissues have been found in curettings and also for the light they throw on the origin of the so-called uterine teratomas.", "contents": "Foetal remnants in the uterus and their relation to other uterine heterotopia. Foetal remnants were found in uterine curettings obtained from three women complaining of irregular vaginal bleeding. These findings are discussed in relation to other cases in the literature in which heterotopic tissues have been found in curettings and also for the light they throw on the origin of the so-called uterine teratomas."}
{"id": "PMID:468135", "title": "The effect of deinstitutionalization on the state hospital.", "content": "Recalling the important statement about the bankruptcy of the state hospital system and the need for a multitude of treatment settings made by Harry Solomon, M.D., in 1958, the authors acknowledge that the changes he prophesized have largely come about. Using the Massachusetts Mental Health Center as the model, they describe how an inpatient unit operates as part of a deinstitutionalized network of services for seriously ill patients. They discuss the five general types of patients admitted and the problems of providing adequate care to such a diverse population. Finally, they make a strong plea for the establishment of regional facilities to care for patients with unusual, complicated problems.", "contents": "The effect of deinstitutionalization on the state hospital. Recalling the important statement about the bankruptcy of the state hospital system and the need for a multitude of treatment settings made by Harry Solomon, M.D., in 1958, the authors acknowledge that the changes he prophesized have largely come about. Using the Massachusetts Mental Health Center as the model, they describe how an inpatient unit operates as part of a deinstitutionalized network of services for seriously ill patients. They discuss the five general types of patients admitted and the problems of providing adequate care to such a diverse population. Finally, they make a strong plea for the establishment of regional facilities to care for patients with unusual, complicated problems."}
{"id": "PMID:468136", "title": "The effect of deinstitutionalization on a general hospital's inpatient psychiatric service.", "content": "As a result of the deinstitutionalization movement, increasing numbers of former state hospital patients are being rehospitalized in general hospital psychiatric units. Because of this change in patient population, the general hospital has had to adjust its treatment strategy to emphasize meticulous review of previous psychiatric history, including medications; plans for meeting the patients' posthospital housing, vocational, and social needs; and development of plans with community care-givers for continuing care. General hospital staff members have to be educated to develop competence in working with the new patient population and a tolerance for chronicity; that can be done through several forums ranging from large staff meetings to individual supervision.", "contents": "The effect of deinstitutionalization on a general hospital's inpatient psychiatric service. As a result of the deinstitutionalization movement, increasing numbers of former state hospital patients are being rehospitalized in general hospital psychiatric units. Because of this change in patient population, the general hospital has had to adjust its treatment strategy to emphasize meticulous review of previous psychiatric history, including medications; plans for meeting the patients' posthospital housing, vocational, and social needs; and development of plans with community care-givers for continuing care. General hospital staff members have to be educated to develop competence in working with the new patient population and a tolerance for chronicity; that can be done through several forums ranging from large staff meetings to individual supervision."}
{"id": "PMID:468137", "title": "Paradoxes in programming for chronic patients in a community clinic.", "content": "In attempting to build a successful program for chronic patients at the Somerville (Mass.) Mental Health Clinic, the authors first found it necessary to uncover, address, and resolve six fundamental paradoxes engendered by deinstitutionalization that were stressful to mental health clinic staff and inhibited effective programming. The paradoxes involve issues relating to community mental health ideology, clinicians' sources of self-esteem and professional ability, and clinicians' views of chronicity and the deinstitutionalization movement in general. Resolution of the paradoxes requires major value changes, which can be brought about by effective clinical leaders who serve as role models and teachers, and who set the tone for patient care.", "contents": "Paradoxes in programming for chronic patients in a community clinic. In attempting to build a successful program for chronic patients at the Somerville (Mass.) Mental Health Clinic, the authors first found it necessary to uncover, address, and resolve six fundamental paradoxes engendered by deinstitutionalization that were stressful to mental health clinic staff and inhibited effective programming. The paradoxes involve issues relating to community mental health ideology, clinicians' sources of self-esteem and professional ability, and clinicians' views of chronicity and the deinstitutionalization movement in general. Resolution of the paradoxes requires major value changes, which can be brought about by effective clinical leaders who serve as role models and teachers, and who set the tone for patient care."}
{"id": "PMID:468138", "title": "From the hospital to the prison: a step forward in deinstitutionalization?", "content": "In Massachusetts there is a growing trend to transfer both direct and indirect mental health service delivery from civil mental hospitals to prison facilities. Three factors associated with deinstitutionalization and a community-based delivery system appear to have contributed to the trend. Those factors are the over-all compromising of programming caused by unitization of state hospitals and the requirement that a full range of psychiatric services be available in every community, the decrease in morale and training of state hospital employees not involved in community treatment, and the lack of outreach to patients in the community who are dangerous or difficult to deal with.", "contents": "From the hospital to the prison: a step forward in deinstitutionalization? In Massachusetts there is a growing trend to transfer both direct and indirect mental health service delivery from civil mental hospitals to prison facilities. Three factors associated with deinstitutionalization and a community-based delivery system appear to have contributed to the trend. Those factors are the over-all compromising of programming caused by unitization of state hospitals and the requirement that a full range of psychiatric services be available in every community, the decrease in morale and training of state hospital employees not involved in community treatment, and the lack of outreach to patients in the community who are dangerous or difficult to deal with."}
{"id": "PMID:468139", "title": "Deinstitutionalization: avoiding the disasters of the past.", "content": "The reasons for the problems created by deinstitutionalization have only recently become clear; they include a lack of consensus about the movement, no real testing of its philosophic bases, the lack of planning for alternative facilities and services (especially for a population with notable social and cognitive deficits), and the inadequacies of the mental health delivery system in general. Providing care for the chronically ill and preparing for future deinstitutionalization means that the issue must be reconceptualized not as one of where people should be housed but as the need to provide the full range of treatments and services that are available in a total institution. Attitudinal and institutional biases and discriminatory practices must be combated, planning for community facilities and services must be improved, and funding for both institutional and community services must be provided during the phasing down of institutional services. The author proposes a set of ten commandments or basic rules to guide future deinstitutionalization activities.", "contents": "Deinstitutionalization: avoiding the disasters of the past. The reasons for the problems created by deinstitutionalization have only recently become clear; they include a lack of consensus about the movement, no real testing of its philosophic bases, the lack of planning for alternative facilities and services (especially for a population with notable social and cognitive deficits), and the inadequacies of the mental health delivery system in general. Providing care for the chronically ill and preparing for future deinstitutionalization means that the issue must be reconceptualized not as one of where people should be housed but as the need to provide the full range of treatments and services that are available in a total institution. Attitudinal and institutional biases and discriminatory practices must be combated, planning for community facilities and services must be improved, and funding for both institutional and community services must be provided during the phasing down of institutional services. The author proposes a set of ten commandments or basic rules to guide future deinstitutionalization activities."}
{"id": "PMID:468140", "title": "An assessment of after-hours visits to a community mental health center.", "content": "Walk-in clinics, originally established in community mental health centers to provide primary and secondary prevention through around-the-clock emergency services, may be serving many people other than those in crisis. The authors conducted an 11-week study of utilization patterns at one center's after-hours walk-in clinc and found that most of the patients were already enrolled in psychotherapy sessions at the center. Some might have been acting out resistance in therapy, the authors theorize. Few patients seen for the first time at the clinic later enrolled in psychotherapy, and few inpatient admissions were made. Although the authors assert that walk-in clinics provide a valuable service, they question whether their original purpose has been distorted and whether the services they provide are always therapeutic.", "contents": "An assessment of after-hours visits to a community mental health center. Walk-in clinics, originally established in community mental health centers to provide primary and secondary prevention through around-the-clock emergency services, may be serving many people other than those in crisis. The authors conducted an 11-week study of utilization patterns at one center's after-hours walk-in clinc and found that most of the patients were already enrolled in psychotherapy sessions at the center. Some might have been acting out resistance in therapy, the authors theorize. Few patients seen for the first time at the clinic later enrolled in psychotherapy, and few inpatient admissions were made. Although the authors assert that walk-in clinics provide a valuable service, they question whether their original purpose has been distorted and whether the services they provide are always therapeutic."}
{"id": "PMID:468146", "title": "Famous works of art create a therapeutic patient environment.", "content": "Works of art by famous American artists hang in patient rooms, in hospital corridors, and in outpatient clinic waiting rooms. An art library allows patients to choose paintings for their rooms, giving them some control over their hospital environment.", "contents": "Famous works of art create a therapeutic patient environment. Works of art by famous American artists hang in patient rooms, in hospital corridors, and in outpatient clinic waiting rooms. An art library allows patients to choose paintings for their rooms, giving them some control over their hospital environment."}
{"id": "PMID:468147", "title": "Health care in the shadow of Old Faithful.", "content": "Yellowstone National Park is served by a unique hospital and clinic system that includes primary care, inpatient stabilization, and professional education for health care professionals.", "contents": "Health care in the shadow of Old Faithful. Yellowstone National Park is served by a unique hospital and clinic system that includes primary care, inpatient stabilization, and professional education for health care professionals."}
{"id": "PMID:468148", "title": "AHA surveys major source of hospital data.", "content": "The Annual Survey of Hospitals and the National Hospital Panel Survey work together to provide a thorough picture of hospital activities.", "contents": "AHA surveys major source of hospital data. The Annual Survey of Hospitals and the National Hospital Panel Survey work together to provide a thorough picture of hospital activities."}
{"id": "PMID:468149", "title": "New life for the country doctor.", "content": "Health Systems Research Institute, a Utah-based hospital and clinic system, is successfully recruiting physicians for rural areas through creation of 'a dispersed group practice'.", "contents": "New life for the country doctor. Health Systems Research Institute, a Utah-based hospital and clinic system, is successfully recruiting physicians for rural areas through creation of 'a dispersed group practice'."}
{"id": "PMID:468150", "title": "Survey shows who is sharing which services.", "content": "An AHA survey shows overall growth in shared services from 1975 to 1978 and participation according to type of hospital, bed-size category, type of service, and other specifics.", "contents": "Survey shows who is sharing which services. An AHA survey shows overall growth in shared services from 1975 to 1978 and participation according to type of hospital, bed-size category, type of service, and other specifics."}
{"id": "PMID:468151", "title": "Insurance plan raises benefits, lowers costs.", "content": "The Direct Service Contract encourages hospital employees to use their own hospital for medical services. The plan benefits the hospital, cuts costs, and raises employee morale.", "contents": "Insurance plan raises benefits, lowers costs. The Direct Service Contract encourages hospital employees to use their own hospital for medical services. The plan benefits the hospital, cuts costs, and raises employee morale."}
{"id": "PMID:468152", "title": "MBO clears way for humanistic approach.", "content": "Social worker became patient service counselor, classification clerk became patient service interviewer, and together they became a team with a more humanistic philosophy.", "contents": "MBO clears way for humanistic approach. Social worker became patient service counselor, classification clerk became patient service interviewer, and together they became a team with a more humanistic philosophy."}
{"id": "PMID:468154", "title": "What's going on in the community?", "content": "Hospitals that are innovators in community health programming were surveyed in order to uncover trends and gain insight into this developing field.", "contents": "What's going on in the community? Hospitals that are innovators in community health programming were surveyed in order to uncover trends and gain insight into this developing field."}
{"id": "PMID:468155", "title": "Marketing: what it is, what it isn't.", "content": "If the marketing concept is to be successfully applied to the health care field, it must be viewed in its entirety. It must be seen as a business function that is tied to the goals of the hospital.", "contents": "Marketing: what it is, what it isn't. If the marketing concept is to be successfully applied to the health care field, it must be viewed in its entirety. It must be seen as a business function that is tied to the goals of the hospital."}
{"id": "PMID:468156", "title": "A new identity can create a new image.", "content": "An image study may show that a hospital is failing to project its strengths to area physicians or may indicate that certain departments need to be redefined.", "contents": "A new identity can create a new image. An image study may show that a hospital is failing to project its strengths to area physicians or may indicate that certain departments need to be redefined."}
{"id": "PMID:468166", "title": "Environmental assessment helps in planning process.", "content": "The AHA's planning document, which identifies factors that affect the hospital industry on a nationwide basis, can be a useful reference for individual hospitals in identifying similar factors that affect them on the local level.", "contents": "Environmental assessment helps in planning process. The AHA's planning document, which identifies factors that affect the hospital industry on a nationwide basis, can be a useful reference for individual hospitals in identifying similar factors that affect them on the local level."}
{"id": "PMID:468168", "title": "Who's going to pay the bill? Health promotion.", "content": "Most people believe that health promotion activities are a good thing, but they see an important need to answer the question of how to finance them.", "contents": "Who's going to pay the bill? Health promotion. Most people believe that health promotion activities are a good thing, but they see an important need to answer the question of how to finance them."}
{"id": "PMID:468169", "title": "Hospital's role expanded with wellness effort. Health promotion.", "content": "A community hospital's \"wellness\" center provides programs in health assessment, health education, and preventive health care.", "contents": "Hospital's role expanded with wellness effort. Health promotion. A community hospital's \"wellness\" center provides programs in health assessment, health education, and preventive health care."}
{"id": "PMID:468170", "title": "Clinic expands adolescents' access to care: Health promotion.", "content": "A Minneapolis health center opened a mini-clinic in a local high school to provide adolescents with better access to care, appropriate referrals, and health education.", "contents": "Clinic expands adolescents' access to care: Health promotion. A Minneapolis health center opened a mini-clinic in a local high school to provide adolescents with better access to care, appropriate referrals, and health education."}
{"id": "PMID:468179", "title": "hospitals adopt new role: Health promotion.", "content": "The American hospital system has made outstanding contributions to the care of the stick. It now faces the challenge of promoting the concept of \"wellness\".", "contents": "hospitals adopt new role: Health promotion. The American hospital system has made outstanding contributions to the care of the stick. It now faces the challenge of promoting the concept of \"wellness\"."}
{"id": "PMID:468180", "title": "Is it a change for the better? Health promotion.", "content": "The public's expectations of hospitals are changing in that they want to know more about taking care of themselves. It is therefore inevitable that hospitals will change.", "contents": "Is it a change for the better? Health promotion. The public's expectations of hospitals are changing in that they want to know more about taking care of themselves. It is therefore inevitable that hospitals will change."}
{"id": "PMID:468181", "title": "Climate ripe for marketing strategies: Health promotion.", "content": "Now is the ideal time for hospitals to promote wellness in the community as part of their marketing strategies.", "contents": "Climate ripe for marketing strategies: Health promotion. Now is the ideal time for hospitals to promote wellness in the community as part of their marketing strategies."}
{"id": "PMID:468191", "title": "Patient relations program offers listening and follow-up.", "content": "Patient relations programs give patients another avenue for communication and complement the services of direct care personnel. At St. Francis Medical Center, Lynwood, CA, administrators give high marks to their two-year-old program staffed by volunteers.", "contents": "Patient relations program offers listening and follow-up. Patient relations programs give patients another avenue for communication and complement the services of direct care personnel. At St. Francis Medical Center, Lynwood, CA, administrators give high marks to their two-year-old program staffed by volunteers."}
{"id": "PMID:468193", "title": "The basis of medicine and religion: respect for persons.", "content": "The nature of the physician-patient relationship underlies the professional's obligation to respect each person. Religion moves those involved in caring for the sick beyond professionalism to a profound sense of common humanity under the Father, of healing as a work of God, and of love as the primary bond with patients.", "contents": "The basis of medicine and religion: respect for persons. The nature of the physician-patient relationship underlies the professional's obligation to respect each person. Religion moves those involved in caring for the sick beyond professionalism to a profound sense of common humanity under the Father, of healing as a work of God, and of love as the primary bond with patients."}
{"id": "PMID:468194", "title": "The self in mission and hospitality.", "content": "The Catholic health care provider's self-definition must be \"servant.\" Individuals and institutions are called upon to avoid selfism and to accept exhilarating tensions between \"going-out\" (mission) and \"welcoming-in\" (hospitality).", "contents": "The self in mission and hospitality. The Catholic health care provider's self-definition must be \"servant.\" Individuals and institutions are called upon to avoid selfism and to accept exhilarating tensions between \"going-out\" (mission) and \"welcoming-in\" (hospitality)."}
{"id": "PMID:468196", "title": "Creation, the eschaton, and bioethics.", "content": "The Christian's role in renewing the universe is to discover the true name of God's creation and to use technology as a serious enterprise of co-creation. Guided by a bioethics based on Gospel values, the Christian healer brings the eschaton closer to realization.", "contents": "Creation, the eschaton, and bioethics. The Christian's role in renewing the universe is to discover the true name of God's creation and to use technology as a serious enterprise of co-creation. Guided by a bioethics based on Gospel values, the Christian healer brings the eschaton closer to realization."}
{"id": "PMID:468197", "title": "MBO works for managers who understand, apply system.", "content": "Where MBO has suceeded, managers thoroughly understood, planned, and adapted the system to their organization's needs. Informed managers in nonprofit organizations can avoid the common mistakes made in business and industry in the 25 years since MBO was first adopted.", "contents": "MBO works for managers who understand, apply system. Where MBO has suceeded, managers thoroughly understood, planned, and adapted the system to their organization's needs. Informed managers in nonprofit organizations can avoid the common mistakes made in business and industry in the 25 years since MBO was first adopted."}
{"id": "PMID:468198", "title": "Handling grievances: guidelines for RNs.", "content": "Top management depends on nurses--even though they are not titled \"supervisors\"--to handle day-to-day grievances and questions arising on the nursing unit.", "contents": "Handling grievances: guidelines for RNs. Top management depends on nurses--even though they are not titled \"supervisors\"--to handle day-to-day grievances and questions arising on the nursing unit."}
{"id": "PMID:468214", "title": "The effect of Public Law 94--484 on pathology residency programs.", "content": "The Health Professions Educational Assistance Act of 1976 (Public Law 94--484) will affect graduate medical programs. The author surveyed directors of medical education in one major metropolitan area to study what the effects might be. With regard to pathology residency programs there are a number of important influences. For example, there will be fewer foreign medical graduates available to study in United States pathology programs. However, those who do come may be of higher quality. Also, owing to financial incentives to increase primary care, medical schools and hospitals may show less interest in the development of speciality programs such as pathology. The changing environment poses challenges to pathology directors to maintain or improve their programs.", "contents": "The effect of Public Law 94--484 on pathology residency programs. The Health Professions Educational Assistance Act of 1976 (Public Law 94--484) will affect graduate medical programs. The author surveyed directors of medical education in one major metropolitan area to study what the effects might be. With regard to pathology residency programs there are a number of important influences. For example, there will be fewer foreign medical graduates available to study in United States pathology programs. However, those who do come may be of higher quality. Also, owing to financial incentives to increase primary care, medical schools and hospitals may show less interest in the development of speciality programs such as pathology. The changing environment poses challenges to pathology directors to maintain or improve their programs."}
{"id": "PMID:468217", "title": "Drug related vasculitis. Clinicopathologic correlations in 30 patients.", "content": "Drug related vasculitis has variously been described as necrotizing hypersensitivity or allergic angiitis or microscopic panarteritis nodosa. We reviewed tissue sections from 30 patients with validated drug hypersensitivity and vasculitis in order to precisely define this entity. No evidence of necrotizing vascular lesions or of fibrinoid associated with necrosis was found. The vascular lesions in all 30 patients involved small arteries, arterioles, capillaries, and venules. The inflammatory infiltrate consisted primarily of mononuclear cells and prominent numbers of eosinophils and was present in all three layers of the involved vessel walls. Clinically the patients developed either localized or systemic vasculitis, which could not be predicted on the basis of the associated drug. The findings of a skin rash, fever, or eosinophilia and the development of symptoms consistent with a hypersensitivity reaction while medication was being taken were all suggestive of the diagnosis of drug related vasculitis.", "contents": "Drug related vasculitis. Clinicopathologic correlations in 30 patients. Drug related vasculitis has variously been described as necrotizing hypersensitivity or allergic angiitis or microscopic panarteritis nodosa. We reviewed tissue sections from 30 patients with validated drug hypersensitivity and vasculitis in order to precisely define this entity. No evidence of necrotizing vascular lesions or of fibrinoid associated with necrosis was found. The vascular lesions in all 30 patients involved small arteries, arterioles, capillaries, and venules. The inflammatory infiltrate consisted primarily of mononuclear cells and prominent numbers of eosinophils and was present in all three layers of the involved vessel walls. Clinically the patients developed either localized or systemic vasculitis, which could not be predicted on the basis of the associated drug. The findings of a skin rash, fever, or eosinophilia and the development of symptoms consistent with a hypersensitivity reaction while medication was being taken were all suggestive of the diagnosis of drug related vasculitis."}
{"id": "PMID:468218", "title": "Juxtaposition of the atrial appendages.", "content": "Juxtaposition of the atrial appendages is a rare congenital cardiac anomaly almost universally associated with severe conotruncal abnormalities, especially transposition of the aorta. This presentation describes a case of right sided juxtaposition of the atrial appendages without concomitant conotruncal malformations. This finding contradicts previous hypotheses concerning the genesis of juxtaposition.", "contents": "Juxtaposition of the atrial appendages. Juxtaposition of the atrial appendages is a rare congenital cardiac anomaly almost universally associated with severe conotruncal abnormalities, especially transposition of the aorta. This presentation describes a case of right sided juxtaposition of the atrial appendages without concomitant conotruncal malformations. This finding contradicts previous hypotheses concerning the genesis of juxtaposition."}
{"id": "PMID:468219", "title": "Focal myositis of the neck.", "content": "Focal myositis is a recently defined, rapidly growing, inflammatory pseudotumor of skeletal muscle. It is characterized by marked myopathic changes of muscle fibers, hypocellular fibrosis of perimysium and endomysium, and an intense inflammatory reaction in the fibrous septa. This report describes the first instance involving the neck muscle of a 40 year old man. A brief discussion of other histologically similar subcutaneous lesions is also presented.", "contents": "Focal myositis of the neck. Focal myositis is a recently defined, rapidly growing, inflammatory pseudotumor of skeletal muscle. It is characterized by marked myopathic changes of muscle fibers, hypocellular fibrosis of perimysium and endomysium, and an intense inflammatory reaction in the fibrous septa. This report describes the first instance involving the neck muscle of a 40 year old man. A brief discussion of other histologically similar subcutaneous lesions is also presented."}
{"id": "PMID:468223", "title": "Pleomorphic pulmonary hamartoma: an apparently unique variant of pulmonary hamartoma.", "content": "Pulmonary hamartomas of limited variety have been described. Most present as asymptomatic coin lesions in adults and consist of mesenchymal tissue, usually cartilage, in combination with irregular spaces lined by epithelium. Another form is found in the neonate and involves large portions or all of a lung. This is associated with a developmental aberration and is best described as a \"congenital adenomatoid malformation\". An apparently unique noninvasive tumor mass was resected from the lung of a middle aged man where it was associated with anomalous lung segmentation and the bronchial and blood supply to the lung. The tumor appeared to be undifferentiated by light microscopic criteria. Neurilemoma, leiomyoma, chordoid tumor, mixed tumor, and neurogenic sarcoma all entered the differential diagnosis. Ultrastructural examination demonstrated a highly complex and unique organization. Such a lesion, apparently developmental in this case, should be recognized and carefully distinguished from the malignant mesenchymal, neurogenic, and teratomatous lesions with which it may be confused. Electron microscopy may be helpful in this regard.", "contents": "Pleomorphic pulmonary hamartoma: an apparently unique variant of pulmonary hamartoma. Pulmonary hamartomas of limited variety have been described. Most present as asymptomatic coin lesions in adults and consist of mesenchymal tissue, usually cartilage, in combination with irregular spaces lined by epithelium. Another form is found in the neonate and involves large portions or all of a lung. This is associated with a developmental aberration and is best described as a \"congenital adenomatoid malformation\". An apparently unique noninvasive tumor mass was resected from the lung of a middle aged man where it was associated with anomalous lung segmentation and the bronchial and blood supply to the lung. The tumor appeared to be undifferentiated by light microscopic criteria. Neurilemoma, leiomyoma, chordoid tumor, mixed tumor, and neurogenic sarcoma all entered the differential diagnosis. Ultrastructural examination demonstrated a highly complex and unique organization. Such a lesion, apparently developmental in this case, should be recognized and carefully distinguished from the malignant mesenchymal, neurogenic, and teratomatous lesions with which it may be confused. Electron microscopy may be helpful in this regard."}
{"id": "PMID:468224", "title": "The pathology of noncirrhotic portal fibrosis: a review of 32 autopsy cases.", "content": "A wide spectrum of clinical and morphologic changes in 32 autopsy cases of noncirrhotic portal fibrosis have been described. The disease frequently occurs in younger patients with a long history of splenomegaly, usually with a history of hematemesis. Females are affected almost equally as often as males in contrast to cirrhosis. The patients tolerate the bleeding episodes well. Death is usually due to massive hemorrhage. The diagnosis is achieved through a process of exclusion. A critical analysis of hemodynamic data, a splenoportogram, liver function tests (particularly Bromsulphalein retention) and angiographic data is mandatory. Needle biopsy of the liver appears to have limited value in making the diagnosis. The gross anatomic findings vary from a nearly normal liver to gross nodularity, seen particularly on the posteroinferior surface. In some cases these nodules are seen to physically impede the portal blood flow and contribute to portal hypertension. Phlebosclerosis of the smaller radicles of the portal vein and irregular scarring are the outstanding morphologic features of the disease. These changes are usually associated with irregular dilatation of some of the larger intrahepatic branches of the portal vein as well as fibroelastosis with or without occluding or organizing thrombi in both intra- and extrahepatic branches of the portal vein. The changes in hepatic venous radicles are characterized by irregular sclerosis, which seems to contribute significantly toward postsinusoidal block in advanced cases. The probable mode of evolution is discussed.", "contents": "The pathology of noncirrhotic portal fibrosis: a review of 32 autopsy cases. A wide spectrum of clinical and morphologic changes in 32 autopsy cases of noncirrhotic portal fibrosis have been described. The disease frequently occurs in younger patients with a long history of splenomegaly, usually with a history of hematemesis. Females are affected almost equally as often as males in contrast to cirrhosis. The patients tolerate the bleeding episodes well. Death is usually due to massive hemorrhage. The diagnosis is achieved through a process of exclusion. A critical analysis of hemodynamic data, a splenoportogram, liver function tests (particularly Bromsulphalein retention) and angiographic data is mandatory. Needle biopsy of the liver appears to have limited value in making the diagnosis. The gross anatomic findings vary from a nearly normal liver to gross nodularity, seen particularly on the posteroinferior surface. In some cases these nodules are seen to physically impede the portal blood flow and contribute to portal hypertension. Phlebosclerosis of the smaller radicles of the portal vein and irregular scarring are the outstanding morphologic features of the disease. These changes are usually associated with irregular dilatation of some of the larger intrahepatic branches of the portal vein as well as fibroelastosis with or without occluding or organizing thrombi in both intra- and extrahepatic branches of the portal vein. The changes in hepatic venous radicles are characterized by irregular sclerosis, which seems to contribute significantly toward postsinusoidal block in advanced cases. The probable mode of evolution is discussed."}
{"id": "PMID:468225", "title": "Chronic glomerular microangiopathy and metastatic carcinoma.", "content": "Two patients with metastatic colonic adenocarcinoma developed deterioration of renal function six and nine months after the diagnosis of malignant disease. A renal biopsy specimen in one case and both postmortem specimens revealed thickening of glomerular capillary loops with focal reduplication of basement membrane-like material. Ultrastructural examination of all three specimens demonstrated a lucent subendothelial zone and no evidence of electron dense deposits. Antifibrinogen staining outlined most capillary loops in one case. It appears that chronic intravascular coagulation induced by the neoplasm was the major pathogenetic process involved in the production of the glomerular lesion in each case.", "contents": "Chronic glomerular microangiopathy and metastatic carcinoma. Two patients with metastatic colonic adenocarcinoma developed deterioration of renal function six and nine months after the diagnosis of malignant disease. A renal biopsy specimen in one case and both postmortem specimens revealed thickening of glomerular capillary loops with focal reduplication of basement membrane-like material. Ultrastructural examination of all three specimens demonstrated a lucent subendothelial zone and no evidence of electron dense deposits. Antifibrinogen staining outlined most capillary loops in one case. It appears that chronic intravascular coagulation induced by the neoplasm was the major pathogenetic process involved in the production of the glomerular lesion in each case."}
{"id": "PMID:468226", "title": "Primary and secondary synovial chondrometaplasia: histopathologic and clinicoradiologic differences.", "content": "One hundred thirty-six cases diagnosed clinically as either synovial chondrometaplasia or osteocartilaginous loose bodies were reviewed. On the basis of the histopathologic peculiarities, the cases were grouped into two distinct categories, primary and secondary synovial chondrometaplasia, comprising 10 and 126 cases, respectively. In each group there was synovial chondrometaplasia but the histologic patterns were dissimilar. In cases of secondary synovial chondrometaplasia the initiating factors were usually obvious and the lesions were nonaggressive. Primary synovial chondrometaplasia, on the other hand, was aggressive and was associated with a high incidence of recurrence.", "contents": "Primary and secondary synovial chondrometaplasia: histopathologic and clinicoradiologic differences. One hundred thirty-six cases diagnosed clinically as either synovial chondrometaplasia or osteocartilaginous loose bodies were reviewed. On the basis of the histopathologic peculiarities, the cases were grouped into two distinct categories, primary and secondary synovial chondrometaplasia, comprising 10 and 126 cases, respectively. In each group there was synovial chondrometaplasia but the histologic patterns were dissimilar. In cases of secondary synovial chondrometaplasia the initiating factors were usually obvious and the lesions were nonaggressive. Primary synovial chondrometaplasia, on the other hand, was aggressive and was associated with a high incidence of recurrence."}
{"id": "PMID:468227", "title": "Papillary tumor of the heart: incidental finding at surgery.", "content": "A large papillary endocardial \"tumor\" of the heart was resected during surgery for closure of an interventricular septal defect in an adult. The theories of pathogenesis of papillary \"tumors\" of the heart are briefly reviewed. To our knowledge, this is the first report of a papillary tumor resected at surgery.", "contents": "Papillary tumor of the heart: incidental finding at surgery. A large papillary endocardial \"tumor\" of the heart was resected during surgery for closure of an interventricular septal defect in an adult. The theories of pathogenesis of papillary \"tumors\" of the heart are briefly reviewed. To our knowledge, this is the first report of a papillary tumor resected at surgery."}
{"id": "PMID:468228", "title": "Villous polyps of the urethra: a report of two cases.", "content": "Two patients with villous polyps of the prostatic urethra are described. The article supports previous observations that the lesion has the morphologic appearance of prostatic tissue. A review of the literature reveals that this rare lesion appears to be specific for the prostatic urethra and behaves as a benign tumor.", "contents": "Villous polyps of the urethra: a report of two cases. Two patients with villous polyps of the prostatic urethra are described. The article supports previous observations that the lesion has the morphologic appearance of prostatic tissue. A review of the literature reveals that this rare lesion appears to be specific for the prostatic urethra and behaves as a benign tumor."}
{"id": "PMID:468231", "title": "Partial trisomy 3q.", "content": "A new case of partial trisomy 3q is reported in a 5-year-old female with severe congenital malformations and psychomotor retardation. A review of the literature, with a total of 11 patients, allows us to conclude that the clinical picture reminiscent of the Cornelia de Lange syndrome is caused by the trisomic state.", "contents": "Partial trisomy 3q. A new case of partial trisomy 3q is reported in a 5-year-old female with severe congenital malformations and psychomotor retardation. A review of the literature, with a total of 11 patients, allows us to conclude that the clinical picture reminiscent of the Cornelia de Lange syndrome is caused by the trisomic state."}
{"id": "PMID:468232", "title": "A case of 21q--syndrome with normal SOD-1 activity.", "content": "A female infant with del(21)(pter leads to q22.1--2:) constitution identified by G, Q, and R banding is reported. She had marked mental and physical retardation, generalized hypertonia, microphthalmia with persistent hypoplastic primary vitreous, blepharochalasia, high nasal bridge, micrognathia, malformed ears with preauricular pits, and overlying fingers. The assay for superoxide dismutase-1 (SOD-1) activity in the patient revealed a normal value and it was suggested that the gene locus for SOD-1 in 21q22.2 leads to qter is not compatible with the presence of the gene dosage effect in the monosomic state.", "contents": "A case of 21q--syndrome with normal SOD-1 activity. A female infant with del(21)(pter leads to q22.1--2:) constitution identified by G, Q, and R banding is reported. She had marked mental and physical retardation, generalized hypertonia, microphthalmia with persistent hypoplastic primary vitreous, blepharochalasia, high nasal bridge, micrognathia, malformed ears with preauricular pits, and overlying fingers. The assay for superoxide dismutase-1 (SOD-1) activity in the patient revealed a normal value and it was suggested that the gene locus for SOD-1 in 21q22.2 leads to qter is not compatible with the presence of the gene dosage effect in the monosomic state."}
{"id": "PMID:468233", "title": "Properties of residual catalase in the erythrocytes of Japanese-type acatalasemia.", "content": "In Japanese-type acatalasemia erythrocytes, the presence and properties of residual catalase were determined and compared with those of normal erythrocyte catalase. Residual catalase activity was proved by titration, active staining after polyacrylamide gel electrophoresis, and measurement of oxygen evolution. Residual catalase protein, demonstrated by double immunodiffusion, was similar to that of normal catalase. The properties of residual catalase activity were identical with those of normal catalase activity. It occurred as three fractions of equal specific activity by DEAE column chromatography. These observations suggest that Japanese-type acatalasemia contains residual catalase with properties similar to those of normal catalase.", "contents": "Properties of residual catalase in the erythrocytes of Japanese-type acatalasemia. In Japanese-type acatalasemia erythrocytes, the presence and properties of residual catalase were determined and compared with those of normal erythrocyte catalase. Residual catalase activity was proved by titration, active staining after polyacrylamide gel electrophoresis, and measurement of oxygen evolution. Residual catalase protein, demonstrated by double immunodiffusion, was similar to that of normal catalase. The properties of residual catalase activity were identical with those of normal catalase activity. It occurred as three fractions of equal specific activity by DEAE column chromatography. These observations suggest that Japanese-type acatalasemia contains residual catalase with properties similar to those of normal catalase."}
{"id": "PMID:468234", "title": "Familial transmission of preauricular fistula in a seven generation Indian pedigree.", "content": "Familial occurrence of fistula auris congenita (ear pits) is described in a Muslin kindred of Indian origin. The pedigree was traced through seven generations. The abnormality appeared in sixty individuals comparising equal number of males and females. No sexual dimorphism was indicated in the expression of the trait, which occurred bilaterally as a small pit just anterior to the crus at the root of the ascending helix. The abnormality was found to be inherited through an autosomal dominant gene with incomplete penetrance. The expression of the trait does not show any dosage effect.", "contents": "Familial transmission of preauricular fistula in a seven generation Indian pedigree. Familial occurrence of fistula auris congenita (ear pits) is described in a Muslin kindred of Indian origin. The pedigree was traced through seven generations. The abnormality appeared in sixty individuals comparising equal number of males and females. No sexual dimorphism was indicated in the expression of the trait, which occurred bilaterally as a small pit just anterior to the crus at the root of the ascending helix. The abnormality was found to be inherited through an autosomal dominant gene with incomplete penetrance. The expression of the trait does not show any dosage effect."}
{"id": "PMID:468235", "title": "Population genetic studies of the Aka pygmies (Central Africa): a survey of red cell and serum enzymes.", "content": "Blood samples collected in a single Pygmy tribe, the Aka, living in Bokoka district (Central African Empire) were investigated with respect to the phenotype and gene frequencies of the following 12 enzyme systems: acid phosphatase, adenosine deaminase, adenylate kinase, carbonic anhydrase, esterase D, glucose-6-phosphate dehydrogenase, malate dehydrogenase, phosphoglucomutase 1, phosphoglucomutase 2, phosphogluconate dehydrogenase, superoxide dismutase and serum cholinesterase variants (locus E1 and E2). The data obtained in the study of genetic polymorphisms of this isolated and inbred population show a specific pattern with the following characteristics: the very low frequency of PGDB and pa alleles; the existence of two rare PGM variants at the PGM2 locus, typical PGM26Pyg (4.2%) and PGM29 (0.2%); the high frequency of the pr allele (10.8%) and CAII2 (8.22%) and ESD2 genes (18.4%). Furthermore, at the G6PD locus four distinct alleles have been found: the negroid GdA- (4%) and GdA+ (16%), the common GdB+ (79.2%)--, and the rare Gd+Ibadan Austin (0.7%). Cholinesterase typings disclosed the presence of the uncommon E1f and E1s genes distributed within a single breeding unit. The results are compared with other data previously reported on South African Khoisan and some Negroid populations; the particular genetic background of Pygmies is discussed.", "contents": "Population genetic studies of the Aka pygmies (Central Africa): a survey of red cell and serum enzymes. Blood samples collected in a single Pygmy tribe, the Aka, living in Bokoka district (Central African Empire) were investigated with respect to the phenotype and gene frequencies of the following 12 enzyme systems: acid phosphatase, adenosine deaminase, adenylate kinase, carbonic anhydrase, esterase D, glucose-6-phosphate dehydrogenase, malate dehydrogenase, phosphoglucomutase 1, phosphoglucomutase 2, phosphogluconate dehydrogenase, superoxide dismutase and serum cholinesterase variants (locus E1 and E2). The data obtained in the study of genetic polymorphisms of this isolated and inbred population show a specific pattern with the following characteristics: the very low frequency of PGDB and pa alleles; the existence of two rare PGM variants at the PGM2 locus, typical PGM26Pyg (4.2%) and PGM29 (0.2%); the high frequency of the pr allele (10.8%) and CAII2 (8.22%) and ESD2 genes (18.4%). Furthermore, at the G6PD locus four distinct alleles have been found: the negroid GdA- (4%) and GdA+ (16%), the common GdB+ (79.2%)--, and the rare Gd+Ibadan Austin (0.7%). Cholinesterase typings disclosed the presence of the uncommon E1f and E1s genes distributed within a single breeding unit. The results are compared with other data previously reported on South African Khoisan and some Negroid populations; the particular genetic background of Pygmies is discussed."}
{"id": "PMID:468236", "title": "'De novo' trisomy 16q11 to pter.", "content": "A de novo 16q- trisomy was found in a patient with severe mental retardation and mild physical abnormalities. A preliminary delineation the clinical features characteristic of trisomy 16q- is proposed, based upon comparison of the two cases identified so far. The mild phenotypical effects of this chromosome imbalance suggest that the alleged lethality of partial chromosome 16 aneuploidies should be reconsidered.", "contents": "'De novo' trisomy 16q11 to pter. A de novo 16q- trisomy was found in a patient with severe mental retardation and mild physical abnormalities. A preliminary delineation the clinical features characteristic of trisomy 16q- is proposed, based upon comparison of the two cases identified so far. The mild phenotypical effects of this chromosome imbalance suggest that the alleged lethality of partial chromosome 16 aneuploidies should be reconsidered."}
{"id": "PMID:468237", "title": "Distribution of glyoxalase I (GLO) variants in Western Europe and the Indian subcontinent.", "content": "English, Italian (including Sardinian), and Spanish populations from Europe and Muslim, Hindu, Sikh, Punjabi, and other populations from the Indian subcontinent currently living either in Birmingham or in India were screened for electrophoretically detectable genetic variants of red cell glyoxalase I (GLO), and their frequencies were reported. All the western European populations investigated, including those reported, exhibited an incidence of close to 44% for the GLO1 gene. The frequency distribution of the GLO1 gene in various populations from the Indian subcontinent, in contrast, was found to range between 0.15 and 0.33. These observations suggest that the European populations in general are genetically more homogeneous than are the populations of the Indian subcontinent.", "contents": "Distribution of glyoxalase I (GLO) variants in Western Europe and the Indian subcontinent. English, Italian (including Sardinian), and Spanish populations from Europe and Muslim, Hindu, Sikh, Punjabi, and other populations from the Indian subcontinent currently living either in Birmingham or in India were screened for electrophoretically detectable genetic variants of red cell glyoxalase I (GLO), and their frequencies were reported. All the western European populations investigated, including those reported, exhibited an incidence of close to 44% for the GLO1 gene. The frequency distribution of the GLO1 gene in various populations from the Indian subcontinent, in contrast, was found to range between 0.15 and 0.33. These observations suggest that the European populations in general are genetically more homogeneous than are the populations of the Indian subcontinent."}
{"id": "PMID:468238", "title": "Sister chromatid exchanges in human leukocyte chromosomes: spontaneous and induced frequencies in early- and late-proliferating cells in vitro.", "content": "Human leukocyte cultures were pulse-treated with the trifunctional alkylating mutagen trenimon in a final concentration of 10(-7) M for 15--20 h after culture start, i.e., in the G1 phase of the cell cycle. At 24 h after culture start bromodeoxyuridine (BUdR) was added to the trenimon-treated cultures and to several untreated cultures running in parallel. The series treated with BUdR only and the series treated with BUdR+trenimon were each used to prepare two cultures at different culture times. Mitoses were collected during consecutive intervals of 12 h from 30 h up to 102 h after culture initiation by colcemid. For all preparation times (42 h, 54 h, 66 h, 78 h, 90 h, and 102 h) the frequencies of first, second, and third and further mitoses were determined in the BUdR- and in the BUdR+trenimon-treated series. In the trenimon-treated series a clear cell cycle delay was detected as compared with the normal distribution of different types of mitoses found in series treated with BUdR only. Spontaneous and trenimon-induced sister chromatid exchange (SCE) frequencies were determined in second mitoses occurring at 66 h, 78 h, 90 h, and 102 h after culture start. For all these preparation times about six SCE per metaphase were consistently found in BUdR-treated, and about 19 SCE per metaphase in BUdR+-trenimon-treated series, indicating a homogeneous sensitivity of early- and late-proliferating cells with respect to the induction of SCE.", "contents": "Sister chromatid exchanges in human leukocyte chromosomes: spontaneous and induced frequencies in early- and late-proliferating cells in vitro. Human leukocyte cultures were pulse-treated with the trifunctional alkylating mutagen trenimon in a final concentration of 10(-7) M for 15--20 h after culture start, i.e., in the G1 phase of the cell cycle. At 24 h after culture start bromodeoxyuridine (BUdR) was added to the trenimon-treated cultures and to several untreated cultures running in parallel. The series treated with BUdR only and the series treated with BUdR+trenimon were each used to prepare two cultures at different culture times. Mitoses were collected during consecutive intervals of 12 h from 30 h up to 102 h after culture initiation by colcemid. For all preparation times (42 h, 54 h, 66 h, 78 h, 90 h, and 102 h) the frequencies of first, second, and third and further mitoses were determined in the BUdR- and in the BUdR+trenimon-treated series. In the trenimon-treated series a clear cell cycle delay was detected as compared with the normal distribution of different types of mitoses found in series treated with BUdR only. Spontaneous and trenimon-induced sister chromatid exchange (SCE) frequencies were determined in second mitoses occurring at 66 h, 78 h, 90 h, and 102 h after culture start. For all these preparation times about six SCE per metaphase were consistently found in BUdR-treated, and about 19 SCE per metaphase in BUdR+-trenimon-treated series, indicating a homogeneous sensitivity of early- and late-proliferating cells with respect to the induction of SCE."}
{"id": "PMID:468239", "title": "Further analysis of the replication bypass model for sister chromatid exchange.", "content": "The replication bypass model for sister chromatid exchange (SCE) proposed by Shafer is examined in detail. When applied through two cell cycles, the model predicts that potentially observable SCEs induced during one S phase will then be cancelled and rendered undetectable during the subsequent S phase. This aspect of replication bypass is inconsistent with the observation of twin SCEs in tetraploid cells. Furthermore, the model cannot account for the efficient induction of SCEs by non-cross-linking chemical agents.", "contents": "Further analysis of the replication bypass model for sister chromatid exchange. The replication bypass model for sister chromatid exchange (SCE) proposed by Shafer is examined in detail. When applied through two cell cycles, the model predicts that potentially observable SCEs induced during one S phase will then be cancelled and rendered undetectable during the subsequent S phase. This aspect of replication bypass is inconsistent with the observation of twin SCEs in tetraploid cells. Furthermore, the model cannot account for the efficient induction of SCEs by non-cross-linking chemical agents."}
{"id": "PMID:468240", "title": "An estimate of genetic risk from 8-methoxypsoralen photochemotherapy.", "content": "A method based on a combination of cell culture and pharmacokinetic data is explored as a way of estimating the possible genetic risk to man from a mutagenic chemical. 8-methoxypsoralen, which is given to psoriasis patients as part of a photochemotherapy regime, is used, since it represents a 'real-life' situation and possesses properties that make it particularly amenable to this approach. It is concluded that treatment of males for a mean period of 10 years before their spermatozoa were involved in conception would increase the gene mutation rate in the male germ line by a factor of 0.00125. The implications of this for the incidence of sex-linked and dominant disease in man are elaborated and the assumptions and limitations are set out. The fragility of the estimates is emphasized.", "contents": "An estimate of genetic risk from 8-methoxypsoralen photochemotherapy. A method based on a combination of cell culture and pharmacokinetic data is explored as a way of estimating the possible genetic risk to man from a mutagenic chemical. 8-methoxypsoralen, which is given to psoriasis patients as part of a photochemotherapy regime, is used, since it represents a 'real-life' situation and possesses properties that make it particularly amenable to this approach. It is concluded that treatment of males for a mean period of 10 years before their spermatozoa were involved in conception would increase the gene mutation rate in the male germ line by a factor of 0.00125. The implications of this for the incidence of sex-linked and dominant disease in man are elaborated and the assumptions and limitations are set out. The fragility of the estimates is emphasized."}
{"id": "PMID:468241", "title": "Cytogenetic effects of replicating and nonreplicating strains of influenza virus on male germ cells of mice.", "content": "Evidence is presented to demonstrate that replicating (A2 and B) and nonreplicating (C) strains of influenza virus were capable of inducing higher percentages of chromosomes anomalies in spermatocytes of mice inoculated either IP or IN than were seen in controls.", "contents": "Cytogenetic effects of replicating and nonreplicating strains of influenza virus on male germ cells of mice. Evidence is presented to demonstrate that replicating (A2 and B) and nonreplicating (C) strains of influenza virus were capable of inducing higher percentages of chromosomes anomalies in spermatocytes of mice inoculated either IP or IN than were seen in controls."}
{"id": "PMID:468242", "title": "Paracentric inversion in the short arm of chromosome 1.", "content": "A paracentric inversion of the short arm of chromosome 1 (inv(1)(p22p36)) is reported in a deeply mentally retarded 19-year-old girl and in her normal father.", "contents": "Paracentric inversion in the short arm of chromosome 1. A paracentric inversion of the short arm of chromosome 1 (inv(1)(p22p36)) is reported in a deeply mentally retarded 19-year-old girl and in her normal father."}
{"id": "PMID:468243", "title": "Partial duplication of 17p. A new chromosomal syndrome.", "content": "An inherited partial duplication syndrome of 17p is described. A comparison of the symptoms of a de novo partial duplication of 17p (Latta and Hoo, 1974) and those of our own case seems to indicate a characteristic syndrome. The main features include a small-for-date baby born at full term, small stature, microcephaly, typical facial changes, a heart defect, contractures of different joints, and deformities of the feet. The patients show severe motor and mental retardation.", "contents": "Partial duplication of 17p. A new chromosomal syndrome. An inherited partial duplication syndrome of 17p is described. A comparison of the symptoms of a de novo partial duplication of 17p (Latta and Hoo, 1974) and those of our own case seems to indicate a characteristic syndrome. The main features include a small-for-date baby born at full term, small stature, microcephaly, typical facial changes, a heart defect, contractures of different joints, and deformities of the feet. The patients show severe motor and mental retardation."}
{"id": "PMID:468244", "title": "Computer-aided analysis of chromosomal aberrations occurring in an abnormal human karyotype.", "content": "A semi-automatic method for recognition of chromosomal changes is presented: from a coded description of an abnormal chromosome (formulated with the aid of an R-banding technique), a computer program makes simulations to reconstitute this chromosomal image from a group of normal chromosomes. Nine types of aberrations (see text) can thus be recognized and the aggregate structure of a group of abnormal chromosomes occurring in a human karyotype can also be rediscovered.", "contents": "Computer-aided analysis of chromosomal aberrations occurring in an abnormal human karyotype. A semi-automatic method for recognition of chromosomal changes is presented: from a coded description of an abnormal chromosome (formulated with the aid of an R-banding technique), a computer program makes simulations to reconstitute this chromosomal image from a group of normal chromosomes. Nine types of aberrations (see text) can thus be recognized and the aggregate structure of a group of abnormal chromosomes occurring in a human karyotype can also be rediscovered."}
{"id": "PMID:468245", "title": "Chromosome distribution studies after inorganic lead exposure.", "content": "We studied the chromosome distribution in persons professionally exposed to inorganic lead. The degree of lead exposure was evaluated by biochemical measurements and cytogenetic analysis. The chromosome distribution was analyzed from trypsin banded karyotypes; in particular we studied centromere distances (delta2) and centromere-metaphase-center distances (d2) which were obtained by computer-aided mathematical transformation of the individual metaphase coordinates. Higher concentrations of blood lead and urine delta-ALA and a statistically significant increase in aneuploidy, hypoploidy, and type-B chromosome aberrations revealed appreciable exposure although none of the subjects showed signs of excessive lead absorption. However study of the chromosome distribution showed no major differences with that of the controls indicating that lead acts preferentially (directly or indirectly) on the chromosomes rather than on the spindle apparatus. A dissociation of the acrocentric chromosomes was observed in the lead group when compared with the controls. This is thought to reflect a secondary action of lead on the nucleolar organiser regions.", "contents": "Chromosome distribution studies after inorganic lead exposure. We studied the chromosome distribution in persons professionally exposed to inorganic lead. The degree of lead exposure was evaluated by biochemical measurements and cytogenetic analysis. The chromosome distribution was analyzed from trypsin banded karyotypes; in particular we studied centromere distances (delta2) and centromere-metaphase-center distances (d2) which were obtained by computer-aided mathematical transformation of the individual metaphase coordinates. Higher concentrations of blood lead and urine delta-ALA and a statistically significant increase in aneuploidy, hypoploidy, and type-B chromosome aberrations revealed appreciable exposure although none of the subjects showed signs of excessive lead absorption. However study of the chromosome distribution showed no major differences with that of the controls indicating that lead acts preferentially (directly or indirectly) on the chromosomes rather than on the spindle apparatus. A dissociation of the acrocentric chromosomes was observed in the lead group when compared with the controls. This is thought to reflect a secondary action of lead on the nucleolar organiser regions."}
{"id": "PMID:468246", "title": "Possible trisomy 1q25 leads to 1q32 in a malformed girl with a de novo insertion in 1q.", "content": "A newborn female is described who exhibited a characteristic facial dysmorphology including deep-set eyes, broad nasal bridge, small mouth, high-arched and narrow palate, severly receding mandible and misshapen ears; constant flexion of the proximal interphalangeal joints, and short distal phalanges and nails of fingers; a congenital heart defect; marked muscular hypotonia, motor and growth retardation. She died at 4 months of age. Her karyotype revealed an additional band in 1q. Banding patterns and clinical picture suggest duplication of the segment 1q25 leads to 1q32.", "contents": "Possible trisomy 1q25 leads to 1q32 in a malformed girl with a de novo insertion in 1q. A newborn female is described who exhibited a characteristic facial dysmorphology including deep-set eyes, broad nasal bridge, small mouth, high-arched and narrow palate, severly receding mandible and misshapen ears; constant flexion of the proximal interphalangeal joints, and short distal phalanges and nails of fingers; a congenital heart defect; marked muscular hypotonia, motor and growth retardation. She died at 4 months of age. Her karyotype revealed an additional band in 1q. Banding patterns and clinical picture suggest duplication of the segment 1q25 leads to 1q32."}
{"id": "PMID:468247", "title": "Tertiary trisomy 14q--, due to paternal balanced translocation 46,XY,t(1;14)(q44;q22).", "content": "Retardation of growth and mental development, craniofacial dysmorphy, limb anomalies, cryptorchidism and repeated infections are observed in a child with 47,XY,+der(14),t(1;14)(q44;q22)pat.", "contents": "Tertiary trisomy 14q--, due to paternal balanced translocation 46,XY,t(1;14)(q44;q22). Retardation of growth and mental development, craniofacial dysmorphy, limb anomalies, cryptorchidism and repeated infections are observed in a child with 47,XY,+der(14),t(1;14)(q44;q22)pat."}
{"id": "PMID:468248", "title": "Partial trisomy 4q in two unrelated cases.", "content": "Two unrelated cases of 4q trisomy are described with trisomic segment 4q25 leads to 4qter. The most conspicuous symptoms are psychomotor retardation, microcephaly, malformed ears, retrognathia, finger and toe malformations and cryptorchism in a male. Both cases are compared with 19 previously reported ones.", "contents": "Partial trisomy 4q in two unrelated cases. Two unrelated cases of 4q trisomy are described with trisomic segment 4q25 leads to 4qter. The most conspicuous symptoms are psychomotor retardation, microcephaly, malformed ears, retrognathia, finger and toe malformations and cryptorchism in a male. Both cases are compared with 19 previously reported ones."}
{"id": "PMID:468249", "title": "Quantitative studies on the arrangement of human metaphase chromosomes. VI. The association pattern of acrocentric chromosomes in patients with trisomy 13.", "content": "The association pattern was studied in 1182 mitoses of 21 patients with trisomy 13 and in a control group. In addition, 173 trisomic mitoses were compared with the same number of diploid mitoses in a case of mosaicism. The number of mitoses with associations was no higher in the trisomic cells than in cells with normal karyotypes. Some differences were observed in the frequency of associations per cell and of the types of associations in the patient group and in the trisomic cells of the mosaic case. The number of associations in which more than two acrocentric chromosomes were involved was unexpectedly low in the cells with a supernumerary chromosome 13. The results are interpreted as suggesting the existence of a compensatory mechanism activated by the additional acrocentric chromosome.", "contents": "Quantitative studies on the arrangement of human metaphase chromosomes. VI. The association pattern of acrocentric chromosomes in patients with trisomy 13. The association pattern was studied in 1182 mitoses of 21 patients with trisomy 13 and in a control group. In addition, 173 trisomic mitoses were compared with the same number of diploid mitoses in a case of mosaicism. The number of mitoses with associations was no higher in the trisomic cells than in cells with normal karyotypes. Some differences were observed in the frequency of associations per cell and of the types of associations in the patient group and in the trisomic cells of the mosaic case. The number of associations in which more than two acrocentric chromosomes were involved was unexpectedly low in the cells with a supernumerary chromosome 13. The results are interpreted as suggesting the existence of a compensatory mechanism activated by the additional acrocentric chromosome."}
{"id": "PMID:468251", "title": "Karyotype instability with multiple 7/14 and 7/7 rearrangements.", "content": "Chromosomes were studied in a mentally retarded boy with microcephaly, growth retardation, facial erythema, caf\u00e9-au-lait spots, and IgA deficiency. In the lymphocytes there was a remarkable tendency to exchange parts of the chromosomes Nos. 7 and 14, the translocations almost exclusively taking place in bands 7p13, 7q32 and 14q11. Seven different types of rearrangements between Nos. 7 and 14, and some other chromosomal aberrations were found. No abnormalities could be detected in the bone marrow. The patient somewhat resembles those affected with ataxia-telangiectasia or with Bloom's syndrome, but on clinical and cytogenetic grounds these disorders could be excluded. 7/14 Translocations similar to those found in our patient's lymphocytes have been reported to occur very rarely in the lymphocyte cultures of individuals with apparently normal chromosome constitution. A relationship between these phenomena may exist.", "contents": "Karyotype instability with multiple 7/14 and 7/7 rearrangements. Chromosomes were studied in a mentally retarded boy with microcephaly, growth retardation, facial erythema, caf\u00e9-au-lait spots, and IgA deficiency. In the lymphocytes there was a remarkable tendency to exchange parts of the chromosomes Nos. 7 and 14, the translocations almost exclusively taking place in bands 7p13, 7q32 and 14q11. Seven different types of rearrangements between Nos. 7 and 14, and some other chromosomal aberrations were found. No abnormalities could be detected in the bone marrow. The patient somewhat resembles those affected with ataxia-telangiectasia or with Bloom's syndrome, but on clinical and cytogenetic grounds these disorders could be excluded. 7/14 Translocations similar to those found in our patient's lymphocytes have been reported to occur very rarely in the lymphocyte cultures of individuals with apparently normal chromosome constitution. A relationship between these phenomena may exist."}
{"id": "PMID:468252", "title": "Human lymphocyte cell cycle: studies with the use of BrUdR.", "content": "The chronological distributions of human blood lymphocytes in first, second and third mitosis following PHA stimulation in vitro are presented. The first G1 phase is shown to be of variable length resulting in some first mitoses appearing only about 150 h after stimulation. The serum or plasma used in the culture medium influences cell cycle time. A total cell cycle time of 10.6 h was estimated for cultures with autologous donor plasma and of 14.7 h for cultures with fetal calf serum. It was further calculated that following PHA stimulation 90% of the lymphocytes divide once, about 65% divide for a second and about 40% divide for a third time.", "contents": "Human lymphocyte cell cycle: studies with the use of BrUdR. The chronological distributions of human blood lymphocytes in first, second and third mitosis following PHA stimulation in vitro are presented. The first G1 phase is shown to be of variable length resulting in some first mitoses appearing only about 150 h after stimulation. The serum or plasma used in the culture medium influences cell cycle time. A total cell cycle time of 10.6 h was estimated for cultures with autologous donor plasma and of 14.7 h for cultures with fetal calf serum. It was further calculated that following PHA stimulation 90% of the lymphocytes divide once, about 65% divide for a second and about 40% divide for a third time."}
{"id": "PMID:468253", "title": "Congenital scalp defects associated with postaxial polydactyly.", "content": "A family is reported, several members of which had congenital scalp defects and postaxial polydactyly type A, with wide variability of expression. The hypothesis is formulated that this association is not fortuitous, but is a distinct malformation complex in which congenital scalp defects are associated with distinct malformation of the limbs.", "contents": "Congenital scalp defects associated with postaxial polydactyly. A family is reported, several members of which had congenital scalp defects and postaxial polydactyly type A, with wide variability of expression. The hypothesis is formulated that this association is not fortuitous, but is a distinct malformation complex in which congenital scalp defects are associated with distinct malformation of the limbs."}
{"id": "PMID:468254", "title": "Partial trisomy 16q resulting from maternal translocation.", "content": "A 3 1/2-year-old male with partial trisomy of the long arm of chromosome 16 resulting from a maternal balanced translocation is described. Karyotype: 46,XY,--22,der(22),t(16;22)(q21;p12)mat.", "contents": "Partial trisomy 16q resulting from maternal translocation. A 3 1/2-year-old male with partial trisomy of the long arm of chromosome 16 resulting from a maternal balanced translocation is described. Karyotype: 46,XY,--22,der(22),t(16;22)(q21;p12)mat."}
{"id": "PMID:468255", "title": "Isodicentric X chromosome in a woman with characteristics of gonadal dysgenesis.", "content": "This paper presents a female patient with primary amenorrhea in whose karyotype an aberrant X chromosome was present. The chromosome resulted from the fusion of two X chromosomes at distal parts of the long arm and from the loss of the segment q24 leads to qter. The clinical and cytogenetic picture is compared with that of patients with the same aberration reported by other authors.", "contents": "Isodicentric X chromosome in a woman with characteristics of gonadal dysgenesis. This paper presents a female patient with primary amenorrhea in whose karyotype an aberrant X chromosome was present. The chromosome resulted from the fusion of two X chromosomes at distal parts of the long arm and from the loss of the segment q24 leads to qter. The clinical and cytogenetic picture is compared with that of patients with the same aberration reported by other authors."}
{"id": "PMID:468256", "title": "A chromosomal abnormality (21q-) in primary thrombocytosis.", "content": "A patient with thrombocytosis was found to present an acquired deletion of the long arm of chrosome 21 (21q-). A similar observation reported in the literature is hereby confirmed.", "contents": "A chromosomal abnormality (21q-) in primary thrombocytosis. A patient with thrombocytosis was found to present an acquired deletion of the long arm of chrosome 21 (21q-). A similar observation reported in the literature is hereby confirmed."}
{"id": "PMID:468257", "title": "Moderate mental retardation and nonspecific dysmorphic syndrome associated with ring chromosome 9.", "content": "A ring chromosome 9 is reported in a 12-year-old, moderately mentally retarded boy. As in other ring chromosome 9 patients, the clinical stigmata are nonspecific and their expressivity is mild. The finding of two normal cells of a total of 121 cells examined favors the hypothesis of a postzygotic, mitotic origin of the ring chromosome formation.", "contents": "Moderate mental retardation and nonspecific dysmorphic syndrome associated with ring chromosome 9. A ring chromosome 9 is reported in a 12-year-old, moderately mentally retarded boy. As in other ring chromosome 9 patients, the clinical stigmata are nonspecific and their expressivity is mild. The finding of two normal cells of a total of 121 cells examined favors the hypothesis of a postzygotic, mitotic origin of the ring chromosome formation."}
{"id": "PMID:468258", "title": "Dicentric Robertsonian translocation in man. 17 cases studied by R,C, and N banding.", "content": "The authors studied 17 cases of Robertsonian translocation. In all cases but one C banding showed that a dicentric translocation was involved. Silver staining demonstrated the presence of an NOR between the two centromeres in only one case.", "contents": "Dicentric Robertsonian translocation in man. 17 cases studied by R,C, and N banding. The authors studied 17 cases of Robertsonian translocation. In all cases but one C banding showed that a dicentric translocation was involved. Silver staining demonstrated the presence of an NOR between the two centromeres in only one case."}
{"id": "PMID:468260", "title": "Increased HK1 activity levels in the red cells of a patient with a de novo trisomy 10p: t(Y;10)(p11;p12).", "content": "A male patient with mental retardation and typical clinical features of 10p trisomy syndrome was found to have a duplication of the short arm of chromosome 10 attached to the short arm of the Y chromosome. Quantitative evaluation of nine red cell enzymes showed significantly increased activity levels of HK1 and, to a lesser extent, of PK, PGI, 6PGD, and G6PD. It is suggested that the HK1 locus may be in the 10pter leads to p12 region. The increased levels of HK1 could affect other erythrocyte metabolic pathways slowing down the physiological rate of cellular senescence and result in increased activity levels of other cell-age-dependent enzymes.", "contents": "Increased HK1 activity levels in the red cells of a patient with a de novo trisomy 10p: t(Y;10)(p11;p12). A male patient with mental retardation and typical clinical features of 10p trisomy syndrome was found to have a duplication of the short arm of chromosome 10 attached to the short arm of the Y chromosome. Quantitative evaluation of nine red cell enzymes showed significantly increased activity levels of HK1 and, to a lesser extent, of PK, PGI, 6PGD, and G6PD. It is suggested that the HK1 locus may be in the 10pter leads to p12 region. The increased levels of HK1 could affect other erythrocyte metabolic pathways slowing down the physiological rate of cellular senescence and result in increased activity levels of other cell-age-dependent enzymes."}
{"id": "PMID:468261", "title": "Position of the human X inactivation center on Xq.", "content": "In three women with a 46,XXq- chromosome constitution, the length of the deletion was expressed as the ratio of the remaining part of Xq to Xp c' over a + b. In one of them (KH) this ratio was 0.33, in another (GE) 0.59, and in the third (AP) the ratio fell between these values. The break in KH is more or less on the border of the Q-dark proximal region. A comparison with relevant X-autosomal translocations indicates that the X inactivation center lies near, but not at the border of, the Q-dark and the adjoining bright region (c and d).", "contents": "Position of the human X inactivation center on Xq. In three women with a 46,XXq- chromosome constitution, the length of the deletion was expressed as the ratio of the remaining part of Xq to Xp c' over a + b. In one of them (KH) this ratio was 0.33, in another (GE) 0.59, and in the third (AP) the ratio fell between these values. The break in KH is more or less on the border of the Q-dark proximal region. A comparison with relevant X-autosomal translocations indicates that the X inactivation center lies near, but not at the border of, the Q-dark and the adjoining bright region (c and d)."}
{"id": "PMID:468262", "title": "Satellite association frequency and number of nucleoli depend on cell cycle duration and NOR-activity. Studies on first, second, and third mitoses of lymphocyte cultures.", "content": "In human lymphocyte cultures the frequencies of satellite associations in first, second, and third mitoses were investigated using the BUDR-method. A marked decrease of the association frequency with increasing numbers of cell cycles was found. The number of nucleoli seen in interphase is correlated with the satellite association frequency in the respective metaphase. Satellite association is positively correlated to Ag-staining intensity of the NORs. Individual differences in satellite association are due to differences in NOR activity and in lymphocyte activation. BUDR diminishes somewhat the Ag-staining intensity of the NORs but has no effect on satellite association frequencies. The main reason for the decrease of satellite association frequency in second and third lymphocyte mitoses is presumably a certain dislocation of the original chromosome position during mitosis and a decreased possibility of association during the short interphases. The high association frequency in first mitosis resembles the chromosome position in the long interphase of G0-lymphocytes.", "contents": "Satellite association frequency and number of nucleoli depend on cell cycle duration and NOR-activity. Studies on first, second, and third mitoses of lymphocyte cultures. In human lymphocyte cultures the frequencies of satellite associations in first, second, and third mitoses were investigated using the BUDR-method. A marked decrease of the association frequency with increasing numbers of cell cycles was found. The number of nucleoli seen in interphase is correlated with the satellite association frequency in the respective metaphase. Satellite association is positively correlated to Ag-staining intensity of the NORs. Individual differences in satellite association are due to differences in NOR activity and in lymphocyte activation. BUDR diminishes somewhat the Ag-staining intensity of the NORs but has no effect on satellite association frequencies. The main reason for the decrease of satellite association frequency in second and third lymphocyte mitoses is presumably a certain dislocation of the original chromosome position during mitosis and a decreased possibility of association during the short interphases. The high association frequency in first mitosis resembles the chromosome position in the long interphase of G0-lymphocytes."}
{"id": "PMID:468263", "title": "Differential function of the phosphoglucomutase isozymes PGM1 and PGM2.", "content": "A total of 13 metabolites thought to be possibly inhibitory were tested for their influence on PGM isozyme activities, each at several different concentrations. The analysis of statistical significance was based on enzyme activities obtained by densitometric measurements of starch gels. Five of the substances were found to inhibit PGM activity, three of which definitely and a further one probably led to a significantly stronger inhibition of the isozymes of the PGM2 locus than of PGM1 isozymes. They are (1) fructose-1,6-diphosphate, (2) adenosine triphosphate, (3) citrate, and (4) possibly 2,3-diphosphoglycerate. Thus, PGM1 isozymes proved to function better in hard or perhaps marginal metabolic conditions. Related evolutionary aspects are discussed.", "contents": "Differential function of the phosphoglucomutase isozymes PGM1 and PGM2. A total of 13 metabolites thought to be possibly inhibitory were tested for their influence on PGM isozyme activities, each at several different concentrations. The analysis of statistical significance was based on enzyme activities obtained by densitometric measurements of starch gels. Five of the substances were found to inhibit PGM activity, three of which definitely and a further one probably led to a significantly stronger inhibition of the isozymes of the PGM2 locus than of PGM1 isozymes. They are (1) fructose-1,6-diphosphate, (2) adenosine triphosphate, (3) citrate, and (4) possibly 2,3-diphosphoglycerate. Thus, PGM1 isozymes proved to function better in hard or perhaps marginal metabolic conditions. Related evolutionary aspects are discussed."}
{"id": "PMID:468270", "title": "Human saliva as a source of biochemical genetic markers. II. Genetic interpretations and possible utilization.", "content": "Human saliva enzymes are compared to analogous blood enzymes. The genetic interpretations for variants of several saliva proteins are reviewed. The possible use of human saliva enzymes and proteins in population genetic studies and disease diagnosis is discussed.", "contents": "Human saliva as a source of biochemical genetic markers. II. Genetic interpretations and possible utilization. Human saliva enzymes are compared to analogous blood enzymes. The genetic interpretations for variants of several saliva proteins are reviewed. The possible use of human saliva enzymes and proteins in population genetic studies and disease diagnosis is discussed."}
{"id": "PMID:468271", "title": "Genetic structure of the Queckchi Indians. Dental microdifferentiation.", "content": "Nine oral morphologic characters were investigated. Their frequencies are compared with those published for other populations. The possibility of using such characters to estimate genetic distance between populations is discussed and the conclusion is reached that, although previous studies have suggested this to be a valid approach, further studies testing this subject are needed.", "contents": "Genetic structure of the Queckchi Indians. Dental microdifferentiation. Nine oral morphologic characters were investigated. Their frequencies are compared with those published for other populations. The possibility of using such characters to estimate genetic distance between populations is discussed and the conclusion is reached that, although previous studies have suggested this to be a valid approach, further studies testing this subject are needed."}
{"id": "PMID:468272", "title": "Sex ratio in man: an analysis of the relationship with ABO blood groups and placental alkaline phosphatase phenotype.", "content": "Secondary sex ratio (SR) in man is influenced by various genetic and environmental factors. It has been observed that SR in subjects of blood group B compatible with their mothers is higher than in other subjects. The analysis of 676 newborns of the Rome population and 1,684 newborns of the New Haven (Connecticut) population have confirmed a higher SR in B group subjects compatible with their mothers. The data also indicate that placental alkaline phosphatase is another genetic factor influencing SR in man and that there is a strong interaction among ABO phenotype, fetomaternal ABO compatible status and PAP phenotype concerning their effects on SR.", "contents": "Sex ratio in man: an analysis of the relationship with ABO blood groups and placental alkaline phosphatase phenotype. Secondary sex ratio (SR) in man is influenced by various genetic and environmental factors. It has been observed that SR in subjects of blood group B compatible with their mothers is higher than in other subjects. The analysis of 676 newborns of the Rome population and 1,684 newborns of the New Haven (Connecticut) population have confirmed a higher SR in B group subjects compatible with their mothers. The data also indicate that placental alkaline phosphatase is another genetic factor influencing SR in man and that there is a strong interaction among ABO phenotype, fetomaternal ABO compatible status and PAP phenotype concerning their effects on SR."}
{"id": "PMID:468273", "title": "Genetic studies on the Kolams of Andhra Pradesh, India.", "content": "A total of 220 persons belonging to the Kolam tribe in the Adilabad District of northwest Andhra Pradesh have been tested for 18 red cell eyzyme systems and for haemoglobin. Generally, the gene frequencies for the systems which showed electrophoretic variation were within the range for Indian populations; the gene frequency for AK1 is high by Indian standards and Hb AS is present. LDH Calcutta 1 was not detected in this population and a single example of PHI 2-1 was observed.", "contents": "Genetic studies on the Kolams of Andhra Pradesh, India. A total of 220 persons belonging to the Kolam tribe in the Adilabad District of northwest Andhra Pradesh have been tested for 18 red cell eyzyme systems and for haemoglobin. Generally, the gene frequencies for the systems which showed electrophoretic variation were within the range for Indian populations; the gene frequency for AK1 is high by Indian standards and Hb AS is present. LDH Calcutta 1 was not detected in this population and a single example of PHI 2-1 was observed."}
{"id": "PMID:468274", "title": "A new method for the analysis of age trends in CPK levels with application to Duchenne muscular dystrophy.", "content": "Measurement of serum creatine phosphokinase (CPK) is the most commonly applied test for carrier detection in Duchenne muscular dystrophy. About two thirds of all carriers have markedly elevated CPK levels. Age correction of CPK measurements would be straightforward if carriers of all ages could be unambiguously identified. Since such identification is impossible, we elaborate an indirect statistical method which is based on Haldane's theory of the balance between selection and mutation for X-linked lethals. We also apply this method to a large body of data gathered on female relatives of Duchenne muscular dystrophy patients and on controls. The results are compared with earlier partial findings.", "contents": "A new method for the analysis of age trends in CPK levels with application to Duchenne muscular dystrophy. Measurement of serum creatine phosphokinase (CPK) is the most commonly applied test for carrier detection in Duchenne muscular dystrophy. About two thirds of all carriers have markedly elevated CPK levels. Age correction of CPK measurements would be straightforward if carriers of all ages could be unambiguously identified. Since such identification is impossible, we elaborate an indirect statistical method which is based on Haldane's theory of the balance between selection and mutation for X-linked lethals. We also apply this method to a large body of data gathered on female relatives of Duchenne muscular dystrophy patients and on controls. The results are compared with earlier partial findings."}
{"id": "PMID:468275", "title": "Aetiological studies of hypospadias in Hungary.", "content": "A complex aetiological (epidemiological, teratological and genetic) study was made in 294 index patients with simple isolated hypospadias. The epidemiological study conducted by the help of personal interviews of the mothers revealed a higher frequency of index patients among children born between August and December, among twins, mainly monozygotic, and finally in the lower birth weight groups and among first-borns. The teratological study showed a higher frequency in mothers of index patients who had had sex hormone treatment, mainly progestogen, before the 16th gestational week. This seems to indicate the aetiological role of fetal androgen deficit caused by the regulation disturbances of human choriogonadotropin. The genetic family study completed by personal examination of first-degree male relatives showed a familial clustering (4.0% versus a point prevalence of 0.44% in live-births). Both the pattern of affected relatives (h2 values 0.62 +/- 0.21 and 0.72 +/- 0.33 in fathers and brothers, respectively, not differing significantly from each other) and further characteristics (other malformations were not frequent in relatives) correspond to the multifactorial-threshold model.", "contents": "Aetiological studies of hypospadias in Hungary. A complex aetiological (epidemiological, teratological and genetic) study was made in 294 index patients with simple isolated hypospadias. The epidemiological study conducted by the help of personal interviews of the mothers revealed a higher frequency of index patients among children born between August and December, among twins, mainly monozygotic, and finally in the lower birth weight groups and among first-borns. The teratological study showed a higher frequency in mothers of index patients who had had sex hormone treatment, mainly progestogen, before the 16th gestational week. This seems to indicate the aetiological role of fetal androgen deficit caused by the regulation disturbances of human choriogonadotropin. The genetic family study completed by personal examination of first-degree male relatives showed a familial clustering (4.0% versus a point prevalence of 0.44% in live-births). Both the pattern of affected relatives (h2 values 0.62 +/- 0.21 and 0.72 +/- 0.33 in fathers and brothers, respectively, not differing significantly from each other) and further characteristics (other malformations were not frequent in relatives) correspond to the multifactorial-threshold model."}
{"id": "PMID:468276", "title": "The inherited blood factors of some Northern Nigerians.", "content": "Results are presented on 147 individuals from northern Nigeria who were tested for the red cell antigens A, A1, B, H, M, N, S, s, He, P1, C, D, Du, E, c, e, Ce, v, Lua, Jka (some for Jkb), Lua, K, Jsa (some for Jsb), Kpa, Rd, Fya and Fyb, and for variants of the serum proteins haptoglobin and transferrin and of the red cell enzymes acid phosphatase, phosphoglucomutase, glucose-6-phosphate dehydrogenase, adenylate kinase, adenosine deaminase, phosphohexose isomerase and lactate dehydrogenase. The results found are of interest as they are among the very few published for this area of Nigeria, but they show little that is unexpected for people living in this region.", "contents": "The inherited blood factors of some Northern Nigerians. Results are presented on 147 individuals from northern Nigeria who were tested for the red cell antigens A, A1, B, H, M, N, S, s, He, P1, C, D, Du, E, c, e, Ce, v, Lua, Jka (some for Jkb), Lua, K, Jsa (some for Jsb), Kpa, Rd, Fya and Fyb, and for variants of the serum proteins haptoglobin and transferrin and of the red cell enzymes acid phosphatase, phosphoglucomutase, glucose-6-phosphate dehydrogenase, adenylate kinase, adenosine deaminase, phosphohexose isomerase and lactate dehydrogenase. The results found are of interest as they are among the very few published for this area of Nigeria, but they show little that is unexpected for people living in this region."}
{"id": "PMID:468278", "title": "New genetic variants of parotid salivary amylase.", "content": "Isoelectric focusing can reveal at least two heterozygotes of the Amy1R class of variants. Family data on these variants show co-dominant inheritance for both alleles with respect to the normal Amy1A allele. The frequency of the alleles Amy1R1 and Amy1R2 in the Dutch population (n = 144) amounts to 0.006 and 0.03, respectively. Electrophoretic analysis of whole parotid saliva from different Amy1R phenotypes and of purified normal and Amy1R1 gene products indicates that the variant proteins differ from the normal protein by enhanced deamidation of asparagine and/or glutamine residues.", "contents": "New genetic variants of parotid salivary amylase. Isoelectric focusing can reveal at least two heterozygotes of the Amy1R class of variants. Family data on these variants show co-dominant inheritance for both alleles with respect to the normal Amy1A allele. The frequency of the alleles Amy1R1 and Amy1R2 in the Dutch population (n = 144) amounts to 0.006 and 0.03, respectively. Electrophoretic analysis of whole parotid saliva from different Amy1R phenotypes and of purified normal and Amy1R1 gene products indicates that the variant proteins differ from the normal protein by enhanced deamidation of asparagine and/or glutamine residues."}
{"id": "PMID:468279", "title": "Lack of enhanced purine biosynthesis in HGPRT- and Lesch-Nyhan cells.", "content": "The rate of de novo purine biosynthesis was measured in a series of hypoxanthine guanine phosphoribosyl transferase deficient (HGPRT-) cells from a variety of sources, including human Lesch-Nyhan cells. Under optimum growth conditions, no enhanced purine biosynthesis was detected (in contrast to previous reports). An 'elevated' level of de novo purine biosynthesis could be detected in mutants following starvation for glutamine. However, this was the result of depression of purine biosynthesis in normal cells, with a resulting artifactual overproduction in mutants.", "contents": "Lack of enhanced purine biosynthesis in HGPRT- and Lesch-Nyhan cells. The rate of de novo purine biosynthesis was measured in a series of hypoxanthine guanine phosphoribosyl transferase deficient (HGPRT-) cells from a variety of sources, including human Lesch-Nyhan cells. Under optimum growth conditions, no enhanced purine biosynthesis was detected (in contrast to previous reports). An 'elevated' level of de novo purine biosynthesis could be detected in mutants following starvation for glutamine. However, this was the result of depression of purine biosynthesis in normal cells, with a resulting artifactual overproduction in mutants."}
{"id": "PMID:468298", "title": "Subcellular localization of the eosinophil chemotactic factor (ECF) and its inactivator in human polymorphonuclear leucocytes (PMN).", "content": "An eosinophil chemotactic factor (ECF) of low MW can be released from human polymorphonuclear leucocytes (PMN) on stimulation with the Ca-ionophore, arachidonic acid and during phagocytosis. After a rapid rise of ECF activity in the supernatant a steep fall of in its activity occurred at the later times of secretion suggesting a mechanism of ECF inactivation. ECF obtained at the later times of secretion represents a stable biological activity and does not decrease on further incubation. In addition, intact PMN and ECF combined do not lead to its inactivation, while incubation of homogenized PMN with ECF decreased its activity. These data suggest the presence of an inactivator for ECF within human PMN. The purpose of the study was to localize ECF and its inactivator within human PMN. After cell disruption, differential and equilibrium gradient centrifugation, subcellular components of human PMN can be obtained which reveal eosinophilotactic (ECF) or ECF-inactivating activity. ECF activity can be recovered (in a structurally bound state) from the microsomal fraction of unstimulated and stimulated PMNs, while another portion is obtainable as a soluble, low mol. wt ECF. The PMN-derived ECF inactivator can be recovered from the peroxidase positive (azurophilic) granules and has a mol. wt of 60,000 and less. We suggest that low mol. wt ECF is derived from the plasma membrane of PMN which can be inactivated by components of the azurophilic granules. The mechanism of inactivation is still unresolved.", "contents": "Subcellular localization of the eosinophil chemotactic factor (ECF) and its inactivator in human polymorphonuclear leucocytes (PMN). An eosinophil chemotactic factor (ECF) of low MW can be released from human polymorphonuclear leucocytes (PMN) on stimulation with the Ca-ionophore, arachidonic acid and during phagocytosis. After a rapid rise of ECF activity in the supernatant a steep fall of in its activity occurred at the later times of secretion suggesting a mechanism of ECF inactivation. ECF obtained at the later times of secretion represents a stable biological activity and does not decrease on further incubation. In addition, intact PMN and ECF combined do not lead to its inactivation, while incubation of homogenized PMN with ECF decreased its activity. These data suggest the presence of an inactivator for ECF within human PMN. The purpose of the study was to localize ECF and its inactivator within human PMN. After cell disruption, differential and equilibrium gradient centrifugation, subcellular components of human PMN can be obtained which reveal eosinophilotactic (ECF) or ECF-inactivating activity. ECF activity can be recovered (in a structurally bound state) from the microsomal fraction of unstimulated and stimulated PMNs, while another portion is obtainable as a soluble, low mol. wt ECF. The PMN-derived ECF inactivator can be recovered from the peroxidase positive (azurophilic) granules and has a mol. wt of 60,000 and less. We suggest that low mol. wt ECF is derived from the plasma membrane of PMN which can be inactivated by components of the azurophilic granules. The mechanism of inactivation is still unresolved."}
{"id": "PMID:468299", "title": "Immune mechanisms against canine distemper. I. Identification of K cell against canine distemper virus infected target cells in vitro.", "content": "Canine peripheral blood lymphocytes, polymorphonuclear leucocytes (PMN) and monocytes (macrophages) were obtained by various cell separation techniques and were tested for their cytotoxic capacity against antibody-sensitized canine distemper virus (CDV) infected Vero cells by an in vitro chromium release assay. Canine lymphocytes were found to destroy CDV infected target cells effectively, while neither PMN nor monocytes (macrophages) could do so. The active lymphocyte was characterized by various rosetting techniques to be a non-T and a non-B lymphocyte. These cells bear no surface immunoglobulin (SIg-) but possessed both Fc receptors (Fc+) and complement receptors (EAC+) suggesting that these cells are neither classical T nor B cells. The possible roles of this K cell in the resistance against canine distemper are discussed.", "contents": "Immune mechanisms against canine distemper. I. Identification of K cell against canine distemper virus infected target cells in vitro. Canine peripheral blood lymphocytes, polymorphonuclear leucocytes (PMN) and monocytes (macrophages) were obtained by various cell separation techniques and were tested for their cytotoxic capacity against antibody-sensitized canine distemper virus (CDV) infected Vero cells by an in vitro chromium release assay. Canine lymphocytes were found to destroy CDV infected target cells effectively, while neither PMN nor monocytes (macrophages) could do so. The active lymphocyte was characterized by various rosetting techniques to be a non-T and a non-B lymphocyte. These cells bear no surface immunoglobulin (SIg-) but possessed both Fc receptors (Fc+) and complement receptors (EAC+) suggesting that these cells are neither classical T nor B cells. The possible roles of this K cell in the resistance against canine distemper are discussed."}
{"id": "PMID:468300", "title": "The production of contact sensitivity by the injection into the footpads of recipients of the lymph node cells from mice 1 day after painting the skin with contact sensitizing agent: requirement for matching at the major histocompatibility complex between donor and recipient mice.", "content": "Donor mice were painted on the skin of the abdomen with the contact sensitizing agent, oxazolone. One day later 2-5 x 10(6) cells from the regional lymph nodes were injected into the footpads of recipient mice. Contact sensitivity was detected 6 days later by challenging the ears of the recipients and measuring the increase of thickness at 24 h. Good contact sensitivity was obtained when CBA cells were injected into CBA mice and BALB/c cells injected into BALB/c mice; the injection of BALB/c (H-2d) cells into CBA (H-2k) mice and vice versa failed to give rise to contact sensitivity. Hybrid F1 cells gave intermediate responses. The contact sensitivity caused by the injection of small numbers of lymph node cells into the footpad is interpreted as a mode of active immunization and the present results show that this only occurs when there is genetic matching at the major histocompatibility complex between the donor and the recipient mouse.", "contents": "The production of contact sensitivity by the injection into the footpads of recipients of the lymph node cells from mice 1 day after painting the skin with contact sensitizing agent: requirement for matching at the major histocompatibility complex between donor and recipient mice. Donor mice were painted on the skin of the abdomen with the contact sensitizing agent, oxazolone. One day later 2-5 x 10(6) cells from the regional lymph nodes were injected into the footpads of recipient mice. Contact sensitivity was detected 6 days later by challenging the ears of the recipients and measuring the increase of thickness at 24 h. Good contact sensitivity was obtained when CBA cells were injected into CBA mice and BALB/c cells injected into BALB/c mice; the injection of BALB/c (H-2d) cells into CBA (H-2k) mice and vice versa failed to give rise to contact sensitivity. Hybrid F1 cells gave intermediate responses. The contact sensitivity caused by the injection of small numbers of lymph node cells into the footpad is interpreted as a mode of active immunization and the present results show that this only occurs when there is genetic matching at the major histocompatibility complex between the donor and the recipient mouse."}
{"id": "PMID:468301", "title": "Growth enhancing property of human monocytes from normal donors and cancer patients.", "content": "Peripheral blood monocytes, but not other leucocytes, from healthy donors, were shown to enhance the DNA synthesis of three cell lines. The effect was very marked on an epithelial (Chang) and lymphoid (CLA-4) cell line derived from normal tissues, and less marked on a carcinoma-derived line (HT-29). The enhancement was demonstrable over a wide range of monocyte:cell line ratios, and some activity was present in supernates from monocytes cultured alone or with a cell line. Furthermore, monocytes from gastric carcinoma patients did not enhance Chang and CLA-4 cells to the same extent, relative to the healthy donors.", "contents": "Growth enhancing property of human monocytes from normal donors and cancer patients. Peripheral blood monocytes, but not other leucocytes, from healthy donors, were shown to enhance the DNA synthesis of three cell lines. The effect was very marked on an epithelial (Chang) and lymphoid (CLA-4) cell line derived from normal tissues, and less marked on a carcinoma-derived line (HT-29). The enhancement was demonstrable over a wide range of monocyte:cell line ratios, and some activity was present in supernates from monocytes cultured alone or with a cell line. Furthermore, monocytes from gastric carcinoma patients did not enhance Chang and CLA-4 cells to the same extent, relative to the healthy donors."}
{"id": "PMID:468302", "title": "High radiosensitivity of induction in contrast to radioresistance of expression of cells mediating delayed-type hypersensitivity during response to sheep red blood cells in mice.", "content": "The delayed-type hypersensitivity DTH) reaction observed in mice primed i.v. with low doses of sheep red blood cells was greatly decreased when mice were irradiated with 300 rad before priming. An estimation of the radiosensitivity of DTH-mediating cells (DTH-C) was performed using a titration assay after local adoptive transfer of these cells mixed with antigen into the footpad of unprimed mice. A high radiosensitivity of the induction of DTH-C was observed with a D37 of approximately 50 rad. In contrast, the expression of DTH-C appeared radioresistant, as the D37 was approximately 2000 rad.", "contents": "High radiosensitivity of induction in contrast to radioresistance of expression of cells mediating delayed-type hypersensitivity during response to sheep red blood cells in mice. The delayed-type hypersensitivity DTH) reaction observed in mice primed i.v. with low doses of sheep red blood cells was greatly decreased when mice were irradiated with 300 rad before priming. An estimation of the radiosensitivity of DTH-mediating cells (DTH-C) was performed using a titration assay after local adoptive transfer of these cells mixed with antigen into the footpad of unprimed mice. A high radiosensitivity of the induction of DTH-C was observed with a D37 of approximately 50 rad. In contrast, the expression of DTH-C appeared radioresistant, as the D37 was approximately 2000 rad."}
{"id": "PMID:468303", "title": "Non-specific immunosuppressive effects of mouse spleen extracts in vitro.", "content": "Cell-free extracts were prepared from the spleens of keyhole limpet haemocyanin (KLH)-primed, ovalbumin (OVA)-primed or unprimed mice, and were shown to suppress non-specifically the secondary antibody response to TNP1000KLH in vitro. Non-specific activity was retained on storage in liquid nitrogen and was not removed by dialysis. These findings are in contrast to those reported elsewhere where it was found that cell-free extracts from the spleens of KLH-primed mice specifically suppressed the IgG response to TNP1000KLH; this discrepancy may well lie in the initial immunological status of the mice used. Nevertheless it is concluded that proper account should be taken of non-specific suppressive effects when studying the activity of cell-free extracts in vitro.", "contents": "Non-specific immunosuppressive effects of mouse spleen extracts in vitro. Cell-free extracts were prepared from the spleens of keyhole limpet haemocyanin (KLH)-primed, ovalbumin (OVA)-primed or unprimed mice, and were shown to suppress non-specifically the secondary antibody response to TNP1000KLH in vitro. Non-specific activity was retained on storage in liquid nitrogen and was not removed by dialysis. These findings are in contrast to those reported elsewhere where it was found that cell-free extracts from the spleens of KLH-primed mice specifically suppressed the IgG response to TNP1000KLH; this discrepancy may well lie in the initial immunological status of the mice used. Nevertheless it is concluded that proper account should be taken of non-specific suppressive effects when studying the activity of cell-free extracts in vitro."}
{"id": "PMID:468306", "title": "Suppression by radioactive strontium of the spontaneous cytotoxicity expressed by adherent, predominantly phagocytic cells from various mouse tissues.", "content": "Adherent, predominantly phagocytic effector cells which have previously been shown to occur in a large number of tissues of mice and rats have the spontaneous capacity to kill a variety of syngeneic, allogeneic and xenogeneic target cells in vitro. The present work shows that 89Sr treatment of mice suppressed this spontaneous cytotoxicity, thus indicating that the effector cells pass a substantial part of their life in the bone marrow. In contrast, the capacity to mobilize mononuclear phagocytes and to enhance cytotoxicity in response to an appropriate stimulus were not consistently impaired.", "contents": "Suppression by radioactive strontium of the spontaneous cytotoxicity expressed by adherent, predominantly phagocytic cells from various mouse tissues. Adherent, predominantly phagocytic effector cells which have previously been shown to occur in a large number of tissues of mice and rats have the spontaneous capacity to kill a variety of syngeneic, allogeneic and xenogeneic target cells in vitro. The present work shows that 89Sr treatment of mice suppressed this spontaneous cytotoxicity, thus indicating that the effector cells pass a substantial part of their life in the bone marrow. In contrast, the capacity to mobilize mononuclear phagocytes and to enhance cytotoxicity in response to an appropriate stimulus were not consistently impaired."}
{"id": "PMID:468308", "title": "Alveolar macrophages. II. Inhibition of lymphocyte proliferation by purified macrophages from rat lung.", "content": "Macrophages were prepared from the lung, peritoneal cavity and blood of normal, unstimulated rats from a number of strains. The macrophages were purified by adherence, and characterized via surface markers, enzyme activity and phagocytic capacity, and subsequently tested for activity in cultures of mitogen-stimulated syngeneic lymphocytes. Peritoneal macrophages and blood monocytes were mildly stimulatory, or ineffective in modulating mitogen-induced DNA synthesis; peritoneal macrophages reconstituted the blastogenic responses of macrophage-depleted lymph node cell cultures to normal limits. In contrast, alveolar macrophages were markedly inhibitory to lymphocyte proliferation; in some instances inhibitory activity was demonstrable when added alveolar macrophages comprised only 0.04% of the total cells in culture. Lymphocyte proliferation induced by T-cell mitogens was more susceptible to this inhibition than was proliferation induced by the B-cell mitogen LPS. Alveolar macrophages recovered from SPF rats, while less in number, exhibited comparable inhibitory activity. These results form part of an emerging picture picture of the normal alveolar macrophage as a potential 'suppressor' of T-cell activity in the lung.", "contents": "Alveolar macrophages. II. Inhibition of lymphocyte proliferation by purified macrophages from rat lung. Macrophages were prepared from the lung, peritoneal cavity and blood of normal, unstimulated rats from a number of strains. The macrophages were purified by adherence, and characterized via surface markers, enzyme activity and phagocytic capacity, and subsequently tested for activity in cultures of mitogen-stimulated syngeneic lymphocytes. Peritoneal macrophages and blood monocytes were mildly stimulatory, or ineffective in modulating mitogen-induced DNA synthesis; peritoneal macrophages reconstituted the blastogenic responses of macrophage-depleted lymph node cell cultures to normal limits. In contrast, alveolar macrophages were markedly inhibitory to lymphocyte proliferation; in some instances inhibitory activity was demonstrable when added alveolar macrophages comprised only 0.04% of the total cells in culture. Lymphocyte proliferation induced by T-cell mitogens was more susceptible to this inhibition than was proliferation induced by the B-cell mitogen LPS. Alveolar macrophages recovered from SPF rats, while less in number, exhibited comparable inhibitory activity. These results form part of an emerging picture picture of the normal alveolar macrophage as a potential 'suppressor' of T-cell activity in the lung."}
{"id": "PMID:468307", "title": "Simultaneous occurrence of hereditary C6 and C2 deficiency in a French-Canadian family.", "content": "The sera of four sisters were found to lack the sixth component of complement (C6) and the serum of one was also partially deficient in the second component (C2). Two other blood relatives were found to be heterozygous for both deficiencies, while only one sibling had normal values. The father of these eight siblings was heterozygous for C2D and C6D and in the third generation, six children were heterozygous for C6 deficiency was treated for chronic active brucel-transmitted; the C6 deficiency was not linked to the HLA system, while the C2-deficiency segregated with the haplotype A10,B18. The proband, homozygous for C6 deficiency was treated for chronic active Brucellosis and in another sibling with C6 deficiency, toxoplasmosis was diagnosed. Neither bleeding disorders nor a tendency to collagen diseases have been observed and the opsonic activity was normal in the sera of all family members.", "contents": "Simultaneous occurrence of hereditary C6 and C2 deficiency in a French-Canadian family. The sera of four sisters were found to lack the sixth component of complement (C6) and the serum of one was also partially deficient in the second component (C2). Two other blood relatives were found to be heterozygous for both deficiencies, while only one sibling had normal values. The father of these eight siblings was heterozygous for C2D and C6D and in the third generation, six children were heterozygous for C6 deficiency was treated for chronic active brucel-transmitted; the C6 deficiency was not linked to the HLA system, while the C2-deficiency segregated with the haplotype A10,B18. The proband, homozygous for C6 deficiency was treated for chronic active Brucellosis and in another sibling with C6 deficiency, toxoplasmosis was diagnosed. Neither bleeding disorders nor a tendency to collagen diseases have been observed and the opsonic activity was normal in the sera of all family members."}
{"id": "PMID:468309", "title": "Differences in [3H]-thymidine uptake of lymph node cells stimulated by Con A and PHA in H-2 congenic mouse strains.", "content": "The genetic control of the lymphocyte responsiveness to Con A and PHA-P has been studied by using inbred, H-2 congenic mouse strains. Segregation studies were carried out on F1 and backcross mice of a high and a low responder strain. According to the results, there is a strong correlation between the responsiveness of lymph node cells to mitogens and H-2 haplotypes in different H-2 congenic strains of mice and in different backcross generations. High responsiveness to PHA-P is associated with H-2b, while low responsiveness with H-2a or H-2k haplotypes, but the correlation is the inverse in response to Con A, in the parental strains and backcross mice as well. The magnitude of the responses to both Con A and PHA-P was found to be intermediate in F1 heterozygotes of a low and high responder parental strain. The effect of other non H-2 genes on the responsiveness to these mitogens has also been demonstrated.", "contents": "Differences in [3H]-thymidine uptake of lymph node cells stimulated by Con A and PHA in H-2 congenic mouse strains. The genetic control of the lymphocyte responsiveness to Con A and PHA-P has been studied by using inbred, H-2 congenic mouse strains. Segregation studies were carried out on F1 and backcross mice of a high and a low responder strain. According to the results, there is a strong correlation between the responsiveness of lymph node cells to mitogens and H-2 haplotypes in different H-2 congenic strains of mice and in different backcross generations. High responsiveness to PHA-P is associated with H-2b, while low responsiveness with H-2a or H-2k haplotypes, but the correlation is the inverse in response to Con A, in the parental strains and backcross mice as well. The magnitude of the responses to both Con A and PHA-P was found to be intermediate in F1 heterozygotes of a low and high responder parental strain. The effect of other non H-2 genes on the responsiveness to these mitogens has also been demonstrated."}
{"id": "PMID:468310", "title": "Inhibition of antibody-dependent cellular cytotoxicity by artificial immune complexes and pathological sera.", "content": "Detection of immune complexes by inhibition of antibody-dependent cellular cytotoxicity (ADCC) is based on the principle that soluble complexes can compete with target cell-bound antibody for receptors (FcR) on cytotoxic lymphocytes. The objective of this study was to define a cytotoxicity system for the determination of soluble immune complexes in the sera of patients with inflammatory bowel disease (IBD). For this purpose, the conditions under which soluble complexes of rat serum albumin (RSA) and rabbit anti-RSA inhibited human K-cell mediated lysis of sensitized Chang cells were examined, on the assumption that the behaviour in the system of circulating immune complexes putatively present in inflammatory bowel disease, is similar to that of artificial immune complexes. Inhibition of ADCC by a standard amount of artificial complex in different normal human sera was relatively uniform provided that the final concentration of the latter did not exceed 10% of the culture medium. In the absence of extraneous complexes, the effect of both normal and IBD sera on ADCC varied widely. Differential inhibition of ADCC by sera from patients with IBD and normal subjects was thus expressed as a function of ADCC in a standard batch of foetal bovine serum (FBS). Under these conditions differences between pathological (n = 51) and normal (n = 52) sera were highly significant (P less than 0.001), which could not be explained by the presence in the patients' sera of HL-A antibodies reactive with the effector cells, nor by a deficit in nutritional support of ADCC. The absence of a correlation between inhibition of ADCC and total serum IgG or IgM inferred that inhibition was attributable to immune complexes in the IBD sera. The limitations of this assay for assessment of the incidence of immune complexes in pathological sera are discussed.", "contents": "Inhibition of antibody-dependent cellular cytotoxicity by artificial immune complexes and pathological sera. Detection of immune complexes by inhibition of antibody-dependent cellular cytotoxicity (ADCC) is based on the principle that soluble complexes can compete with target cell-bound antibody for receptors (FcR) on cytotoxic lymphocytes. The objective of this study was to define a cytotoxicity system for the determination of soluble immune complexes in the sera of patients with inflammatory bowel disease (IBD). For this purpose, the conditions under which soluble complexes of rat serum albumin (RSA) and rabbit anti-RSA inhibited human K-cell mediated lysis of sensitized Chang cells were examined, on the assumption that the behaviour in the system of circulating immune complexes putatively present in inflammatory bowel disease, is similar to that of artificial immune complexes. Inhibition of ADCC by a standard amount of artificial complex in different normal human sera was relatively uniform provided that the final concentration of the latter did not exceed 10% of the culture medium. In the absence of extraneous complexes, the effect of both normal and IBD sera on ADCC varied widely. Differential inhibition of ADCC by sera from patients with IBD and normal subjects was thus expressed as a function of ADCC in a standard batch of foetal bovine serum (FBS). Under these conditions differences between pathological (n = 51) and normal (n = 52) sera were highly significant (P less than 0.001), which could not be explained by the presence in the patients' sera of HL-A antibodies reactive with the effector cells, nor by a deficit in nutritional support of ADCC. The absence of a correlation between inhibition of ADCC and total serum IgG or IgM inferred that inhibition was attributable to immune complexes in the IBD sera. The limitations of this assay for assessment of the incidence of immune complexes in pathological sera are discussed."}
{"id": "PMID:468311", "title": "The transport by hepatocytes of immunoglobulin A from blood to bile visualized by autoradiography and electron microscopy.", "content": "Polymeric myeloma IgA, labelled with 125I, was injected intravenously into rats that were killed 5, 30, or 60 min later and the livers removed, fixed and sectioned. Autoradiographs of ultra-thin sections examined in the electron microscope showed that the IgA first became bound to the plasma membrane of the hepatocytes but after 30 min much of it was transported across their cytoplasm and became localized around the bile canaliculi. At this time, autoradiographs of 1 micrometer sections examined in the light microscope showed the contents of the bile ducts in the portal tracts to be labelled heavily. These results confirm the previous finding of rapid transport of IgA across the liver and show directly that the hepatocytes are the cells that carry it out. No intracellular organelle or vesicular structure, discernible within the resolving power of the techniques used, could be implicated in the transport mechanism.", "contents": "The transport by hepatocytes of immunoglobulin A from blood to bile visualized by autoradiography and electron microscopy. Polymeric myeloma IgA, labelled with 125I, was injected intravenously into rats that were killed 5, 30, or 60 min later and the livers removed, fixed and sectioned. Autoradiographs of ultra-thin sections examined in the electron microscope showed that the IgA first became bound to the plasma membrane of the hepatocytes but after 30 min much of it was transported across their cytoplasm and became localized around the bile canaliculi. At this time, autoradiographs of 1 micrometer sections examined in the light microscope showed the contents of the bile ducts in the portal tracts to be labelled heavily. These results confirm the previous finding of rapid transport of IgA across the liver and show directly that the hepatocytes are the cells that carry it out. No intracellular organelle or vesicular structure, discernible within the resolving power of the techniques used, could be implicated in the transport mechanism."}
{"id": "PMID:468312", "title": "Soluble oligovalent antigen-antibody complexes. I. The effect of antigen valence and combining ratio on the composition of fluorescein-carrier anti-fluorescein complexes.", "content": "Soluble oligovalent antigen--antibody complexes were prepared and analysed by ultracentrifugation in order to study the effect of the combining ratio, antigen valence and concentration upon the size and molecular composition of the composition of the complexes. Fluorescein (F) conjugates of rabbit serum albumin (RSA) and thyroglobulin (RTg) were combined with high affinity rabbit anti-F antibodies to form soluble complexes. The effect of the combining ratio paralleled findings in precipitating systems in that the largest soluble complexes were found at equimolarity and mild molar antibody excess. Tetravalent antigen formed precipitates at combining ratios near equimolarity, whereas trivalent antigens failed to precipitate at similar concentrations. Complexes prepared near equimolarity were most sensitive to changes in concentration, higher concentrations leading to larger complexes. The Ab/Ag ratios of different-size complexes in the same preparation were remarkably similar. This ratio was dependent on the antibody--antigen combining ratio, was limited by antigen valence and was not affected by concentration differences. The data support the hypothesis that soluble complexes are formed in two steps. First, antigen and antibody combine to form subunits whose Ab/Ag ratio is determined by the combining ratio and antigen valence. These subunits then combine to form larger complexes in a manner analogous to polymerization.", "contents": "Soluble oligovalent antigen-antibody complexes. I. The effect of antigen valence and combining ratio on the composition of fluorescein-carrier anti-fluorescein complexes. Soluble oligovalent antigen--antibody complexes were prepared and analysed by ultracentrifugation in order to study the effect of the combining ratio, antigen valence and concentration upon the size and molecular composition of the composition of the complexes. Fluorescein (F) conjugates of rabbit serum albumin (RSA) and thyroglobulin (RTg) were combined with high affinity rabbit anti-F antibodies to form soluble complexes. The effect of the combining ratio paralleled findings in precipitating systems in that the largest soluble complexes were found at equimolarity and mild molar antibody excess. Tetravalent antigen formed precipitates at combining ratios near equimolarity, whereas trivalent antigens failed to precipitate at similar concentrations. Complexes prepared near equimolarity were most sensitive to changes in concentration, higher concentrations leading to larger complexes. The Ab/Ag ratios of different-size complexes in the same preparation were remarkably similar. This ratio was dependent on the antibody--antigen combining ratio, was limited by antigen valence and was not affected by concentration differences. The data support the hypothesis that soluble complexes are formed in two steps. First, antigen and antibody combine to form subunits whose Ab/Ag ratio is determined by the combining ratio and antigen valence. These subunits then combine to form larger complexes in a manner analogous to polymerization."}
{"id": "PMID:468313", "title": "Soluble oligovalent antigen--antibody complexes. II. The effect of various selective forces upon relative stability of isolated complexes.", "content": "Soluble oligovalent antigen--antibody complexes were isolated and analysed by ultracentrifugation to assess the effect of several forces upon the composition and stability of soluble complexes. Complexes were prepared with fluorescein (F) conjugates of rabbit serum albumin (RSA) or thyroglobulin (RTg) and high affinity rabbit anti-F antibodies. Isolated complexes containing two antigen molecules (Ag2 complexes) tended to dissociate and form an equilibrium with complexes containing one antigen molecule (Ag1 complexes). This equilibrium was thermolabile, concentration dependent and affected by the original combining ratio and the area in the gradient from which complexes were harvested. Small amounts of free antibody dissociated from soluble complexes also to form a dynamic equilibrium; this equilibrium was much less affected by the above parameters. The data support the concept that complexes grow in size by a process analogous to polymerization of simple subunits and that the driving forces for polymerization are of a lower order of magnitude and more affected by physical variables than the primary reaction between antibody and its antigen.", "contents": "Soluble oligovalent antigen--antibody complexes. II. The effect of various selective forces upon relative stability of isolated complexes. Soluble oligovalent antigen--antibody complexes were isolated and analysed by ultracentrifugation to assess the effect of several forces upon the composition and stability of soluble complexes. Complexes were prepared with fluorescein (F) conjugates of rabbit serum albumin (RSA) or thyroglobulin (RTg) and high affinity rabbit anti-F antibodies. Isolated complexes containing two antigen molecules (Ag2 complexes) tended to dissociate and form an equilibrium with complexes containing one antigen molecule (Ag1 complexes). This equilibrium was thermolabile, concentration dependent and affected by the original combining ratio and the area in the gradient from which complexes were harvested. Small amounts of free antibody dissociated from soluble complexes also to form a dynamic equilibrium; this equilibrium was much less affected by the above parameters. The data support the concept that complexes grow in size by a process analogous to polymerization of simple subunits and that the driving forces for polymerization are of a lower order of magnitude and more affected by physical variables than the primary reaction between antibody and its antigen."}
{"id": "PMID:468369", "title": "Kinetics of different specific immunological parameters after rabies vaccination in mice.", "content": "A significant protection to an intracerebral challenge of 70 mean lethal doses of a standard live rabies virus strain was obtained in BCG-pretreated mice or in normal mice which had been immunized with a single subcutaneous injection of a beta-propiolactone-inactivated rabies vaccine. Concomitantly, levels of delayed-type hypersensitivity (measured in vivo by the footpad test) and serum-neutralizing activity were evaluated at various times after immunization. All immune criteria were significantly augmented in the BCG-pretreated, rabies-immune mice as compared to normal, rabies-immune mice. However, peak levels of protection, delayed-type hypersensitivity, and serum-neutralizing activity did not occur at the same times. For instance, in the BCG-pretreated, rabies-immune mice, delayed-type hypersensitivity peaked on day 7, protection peaked on day 21, and serum-neutralizing activity peaked on day 60. In BCG-pretreated mice, which did not receive the rabies vaccine, positive delayed-type hypersensitivity, some protection, and serum neutralizing activity were observed 4 to 5 weeks after BCG pretreatment. The possible relationships between specific and nonspecific immunity provoked by rabies virus antigens, tissue culture cell-associated antigens (derived from the bovine fetal kidney cells in which the rabies virus was grown, and BCG are discussed.", "contents": "Kinetics of different specific immunological parameters after rabies vaccination in mice. A significant protection to an intracerebral challenge of 70 mean lethal doses of a standard live rabies virus strain was obtained in BCG-pretreated mice or in normal mice which had been immunized with a single subcutaneous injection of a beta-propiolactone-inactivated rabies vaccine. Concomitantly, levels of delayed-type hypersensitivity (measured in vivo by the footpad test) and serum-neutralizing activity were evaluated at various times after immunization. All immune criteria were significantly augmented in the BCG-pretreated, rabies-immune mice as compared to normal, rabies-immune mice. However, peak levels of protection, delayed-type hypersensitivity, and serum-neutralizing activity did not occur at the same times. For instance, in the BCG-pretreated, rabies-immune mice, delayed-type hypersensitivity peaked on day 7, protection peaked on day 21, and serum-neutralizing activity peaked on day 60. In BCG-pretreated mice, which did not receive the rabies vaccine, positive delayed-type hypersensitivity, some protection, and serum neutralizing activity were observed 4 to 5 weeks after BCG pretreatment. The possible relationships between specific and nonspecific immunity provoked by rabies virus antigens, tissue culture cell-associated antigens (derived from the bovine fetal kidney cells in which the rabies virus was grown, and BCG are discussed."}
{"id": "PMID:468370", "title": "Inactivation or elimination of potentially trypanolytic, complement-activating immune complexes by pathogenic trypanosomes.", "content": "Trypanosoma brucei subsp. brucei and T. congolense incubated in homologous antibody at 0 degrees C for 30 min were lysed by subsequent addition of guinea pig complement. Trypanosomes incubated in antibody at 37 degrees C before complement treatment remained intact. Parasites bearing adsorbed antibody also remained intact when incubated at 37 degrees C before complement treatment. The proportion of cells which survived complement treatment decreased with increasing antibody concentration. Parasites which survived complement treatment continued to express antigens which could bind complement-activating antibody, but did not bear complement-activating immune complexes. Virtually all cells in T. brucei populations exposed to antibody at 37 degrees C, but only 10(-3) to 10(-5) of the cells in populations exposed to antibody at 0 degrees C before complement treatment, remained infective for X-irradiated mice. Only 10(-6) to 10(-7) of T. brucei populations exposed to antibody at 0 or 37 degrees C before complement treatment infected mice immunized with homologous antigens. Serotype analysis of substrains and of T. brucei populations isolated from mice infected with antibody and complement-treated parasites suggested that variant-specific antigens participated in trypanolysis and that T. brucei which survived complement treatment could undergo antigenic variation. Mechanisms by which trypanosomes may inactivate or eliminate surface immune complexes and the possible significance of this phenomenon in trypanosomiasis are discussed.", "contents": "Inactivation or elimination of potentially trypanolytic, complement-activating immune complexes by pathogenic trypanosomes. Trypanosoma brucei subsp. brucei and T. congolense incubated in homologous antibody at 0 degrees C for 30 min were lysed by subsequent addition of guinea pig complement. Trypanosomes incubated in antibody at 37 degrees C before complement treatment remained intact. Parasites bearing adsorbed antibody also remained intact when incubated at 37 degrees C before complement treatment. The proportion of cells which survived complement treatment decreased with increasing antibody concentration. Parasites which survived complement treatment continued to express antigens which could bind complement-activating antibody, but did not bear complement-activating immune complexes. Virtually all cells in T. brucei populations exposed to antibody at 37 degrees C, but only 10(-3) to 10(-5) of the cells in populations exposed to antibody at 0 degrees C before complement treatment, remained infective for X-irradiated mice. Only 10(-6) to 10(-7) of T. brucei populations exposed to antibody at 0 or 37 degrees C before complement treatment infected mice immunized with homologous antigens. Serotype analysis of substrains and of T. brucei populations isolated from mice infected with antibody and complement-treated parasites suggested that variant-specific antigens participated in trypanolysis and that T. brucei which survived complement treatment could undergo antigenic variation. Mechanisms by which trypanosomes may inactivate or eliminate surface immune complexes and the possible significance of this phenomenon in trypanosomiasis are discussed."}
{"id": "PMID:468371", "title": "Interaction of alphaviruses with human peripheral leukocytes: in vitro replication of Venezuelan equine encephalomyelitis virus in monocyte cultures.", "content": "Human peripheral blood leukocytes (PBL) were examined for their ability to support growth of several group A arboviruses in vitro. Cells were refractory to infection with eastern (EEE) and western (WEE) equine encephalitis viruses, whereas Venezuelan equine encephalomyelitis (VEE) virus was shown to infect and replicate to a substantially high titer. When PBL were fractionated into purified subpopulations, only the monocytes were susceptible to predictive VEE virus infection. Lymphocytes treated 24 h before virus inoculation with phytohemagglutinin (10 microgram/ml) were capable of propagating significant amounts of VEE virus. A monocytic cell line, J-111, was also susceptible to infection with VEE, EEE, and WEE viruses, whereas a lymphocytic cell line, Raji, was refractory. Additional information on the participation of PBL during human infection with these viruses may add considerably to our understanding of their differing pathogenicities and clinical pictures.", "contents": "Interaction of alphaviruses with human peripheral leukocytes: in vitro replication of Venezuelan equine encephalomyelitis virus in monocyte cultures. Human peripheral blood leukocytes (PBL) were examined for their ability to support growth of several group A arboviruses in vitro. Cells were refractory to infection with eastern (EEE) and western (WEE) equine encephalitis viruses, whereas Venezuelan equine encephalomyelitis (VEE) virus was shown to infect and replicate to a substantially high titer. When PBL were fractionated into purified subpopulations, only the monocytes were susceptible to predictive VEE virus infection. Lymphocytes treated 24 h before virus inoculation with phytohemagglutinin (10 microgram/ml) were capable of propagating significant amounts of VEE virus. A monocytic cell line, J-111, was also susceptible to infection with VEE, EEE, and WEE viruses, whereas a lymphocytic cell line, Raji, was refractory. Additional information on the participation of PBL during human infection with these viruses may add considerably to our understanding of their differing pathogenicities and clinical pictures."}
{"id": "PMID:468372", "title": "Depression of Rauscher leukemia virus envelope glycoprotein gp71 binding by lymphoid cells during leukemogenesis in mice.", "content": "The availability of membrane receptors for the 71,000-dalton envelope glycoprotein (gp71) of Rauscher murine leukemia virus on splenic and thymic cells from BALB/c mice during Rauscher murine leukemia virus-induced leukemogenesis was determined utilizing a radiolabeled gp71 binding assay. Shortly after infection, the relative cellular [125I]gp71 binding level decreased, first with splenic cells (at day 7 to 10 after infection) and later with thymic cells (at day 10 to 20 after infection). The dependency of the reduction of binding on the replication of the inoculated virus was demonstrated by regression analyses using cellular gp71 binding level as the dependent variable and infectious virus titer, as well as viral gp71 and p30 levels, of spleens and thymuses from infected mice as independent variables. With each independent variable, the reduction of gp71 binding for both cell types was highly dependent (P less than 0.01) on the level of virus detected in their respective organ. In the early stages of leukemogenesis, the [125I]gp71 binding level declined to approximately 20 to 30% of control values. During this period the rate of reduction of binding was very rapid and, in general was similar for both splenic and thymic cells. Further progression of the disease resulted in little or no further reduction in binding. The application of this technique to monitor host ecotropic virus synthesis and to study cell surface virus receptor control mechanisms in vivo is discussed.", "contents": "Depression of Rauscher leukemia virus envelope glycoprotein gp71 binding by lymphoid cells during leukemogenesis in mice. The availability of membrane receptors for the 71,000-dalton envelope glycoprotein (gp71) of Rauscher murine leukemia virus on splenic and thymic cells from BALB/c mice during Rauscher murine leukemia virus-induced leukemogenesis was determined utilizing a radiolabeled gp71 binding assay. Shortly after infection, the relative cellular [125I]gp71 binding level decreased, first with splenic cells (at day 7 to 10 after infection) and later with thymic cells (at day 10 to 20 after infection). The dependency of the reduction of binding on the replication of the inoculated virus was demonstrated by regression analyses using cellular gp71 binding level as the dependent variable and infectious virus titer, as well as viral gp71 and p30 levels, of spleens and thymuses from infected mice as independent variables. With each independent variable, the reduction of gp71 binding for both cell types was highly dependent (P less than 0.01) on the level of virus detected in their respective organ. In the early stages of leukemogenesis, the [125I]gp71 binding level declined to approximately 20 to 30% of control values. During this period the rate of reduction of binding was very rapid and, in general was similar for both splenic and thymic cells. Further progression of the disease resulted in little or no further reduction in binding. The application of this technique to monitor host ecotropic virus synthesis and to study cell surface virus receptor control mechanisms in vivo is discussed."}
{"id": "PMID:468373", "title": "Purification and characterization of different types of exfoliative toxin from Staphylococcus aureus.", "content": "Exfoliative toxin was isolated from strain DI of Staphylococcus aureus using carboxymethyl cellulose and hydroxylapatite chromatography. This purified toxin was compared with that produced by strain TA. The specific biological activity of the two toxins was the same, but they were serologically distinct. These strains have different loci (plasmid or chromosomal) for toxin production; differences were seen in molecular weight and amino acid composition. N-terminal amino acid sequences from the two strains showed significant homology using a single unit alignment shift.", "contents": "Purification and characterization of different types of exfoliative toxin from Staphylococcus aureus. Exfoliative toxin was isolated from strain DI of Staphylococcus aureus using carboxymethyl cellulose and hydroxylapatite chromatography. This purified toxin was compared with that produced by strain TA. The specific biological activity of the two toxins was the same, but they were serologically distinct. These strains have different loci (plasmid or chromosomal) for toxin production; differences were seen in molecular weight and amino acid composition. N-terminal amino acid sequences from the two strains showed significant homology using a single unit alignment shift."}
{"id": "PMID:468374", "title": "Interaction of Chlamydia psittaci reticulate bodies with mouse peritoneal macrophages.", "content": "Noninfectious reticulate bodies of Chlamydia psittaci are readily phagocytized by thioglycolate-elicited mouse peritoneal macrophages in monolayer culture. The internalized reticulate bodies are rapidly destroyed as indicated by a 60 to 70% decrease in trichloroacetic acid-precipitable radioisotopic counts in the macrophage pellet by 10 h and a concomitant increase of the trichloroacetic acid-soluble radiolabeled chlamydial nucleic acid in the cytoplasm. This intracellular destruction of reticulate bodies in macrophages is independent of the multiplicity of infection. Reticulate bodies at a high multiplicity of infection, up to 1,000:1, are also incapable of inducing immediate cytotoxicity in macrophages as evidenced by the lack of early release of the host cell-soluble cytoplasmic enzyme lactic dehydrogenase. Thus, it appears that the virulence factors for (i) initiation or maintenance of intracellular survival via circumvention of phagolysosome formation and (ii) host cell damage are either missing or not expressed by the RB form of this bacterium.", "contents": "Interaction of Chlamydia psittaci reticulate bodies with mouse peritoneal macrophages. Noninfectious reticulate bodies of Chlamydia psittaci are readily phagocytized by thioglycolate-elicited mouse peritoneal macrophages in monolayer culture. The internalized reticulate bodies are rapidly destroyed as indicated by a 60 to 70% decrease in trichloroacetic acid-precipitable radioisotopic counts in the macrophage pellet by 10 h and a concomitant increase of the trichloroacetic acid-soluble radiolabeled chlamydial nucleic acid in the cytoplasm. This intracellular destruction of reticulate bodies in macrophages is independent of the multiplicity of infection. Reticulate bodies at a high multiplicity of infection, up to 1,000:1, are also incapable of inducing immediate cytotoxicity in macrophages as evidenced by the lack of early release of the host cell-soluble cytoplasmic enzyme lactic dehydrogenase. Thus, it appears that the virulence factors for (i) initiation or maintenance of intracellular survival via circumvention of phagolysosome formation and (ii) host cell damage are either missing or not expressed by the RB form of this bacterium."}
{"id": "PMID:468375", "title": "Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis.", "content": "A solid-phase radioimmunoassay (RIA) was used for the detection and quantitation of immunoglobulin class-specific antibodies to Mycoplasma pulmonis in mouse sera. The RIA was found to be more sensitive than the single radial haemolysis (SRH) test for detecting antibodies in sera from mice at intervals after inoculation with M. pulmonis, since antibody was detected in 26 out of 29 serum samples by RIA but in only 8 out of 29 samples by SRH. A method is described for quantitating immunoglobulin class-specific antibody in test sera by reference to a standard serum. The RIA should be useful for the study of the relative importance of various immunoglobulin classes in resistance to mycoplasma infections.", "contents": "Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis. A solid-phase radioimmunoassay (RIA) was used for the detection and quantitation of immunoglobulin class-specific antibodies to Mycoplasma pulmonis in mouse sera. The RIA was found to be more sensitive than the single radial haemolysis (SRH) test for detecting antibodies in sera from mice at intervals after inoculation with M. pulmonis, since antibody was detected in 26 out of 29 serum samples by RIA but in only 8 out of 29 samples by SRH. A method is described for quantitating immunoglobulin class-specific antibody in test sera by reference to a standard serum. The RIA should be useful for the study of the relative importance of various immunoglobulin classes in resistance to mycoplasma infections."}
{"id": "PMID:468376", "title": "Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii.", "content": "Coaggregation reactions between actinomycete and streptococcal cells occurred frequently when human strains of Actinomyces viscosus or A. naeslundii were mixed with human isolates of Streptococcus sanguis or S. mitis, but were infrequent with other oral actinomycetes and streptococci. Two groups of actinomycetes and four groups of streptococci were defined by the patterns of their coaggregation reactions and by the ability of beta-linked galactosides (i.e., lactose) to reverse these reactions. Coaggregations occurred by one of the following three kinds to cell-cell interactions: (i) coaggregation that was blocked by heating the streptococcus but not the actinomycete and was not reversed by lactose; (ii) coaggregation that was blocked by heating the actinomycete but not the streptococcus and was reversed by lactose; and (iii) coaggregation that was blocked only by heating both cell types. The latter reaction was a combination of the first two since lactose reversed coaggregation between heated streptococci and unheated actinomycetes but did not reverse coaggregations between unheated streptococci and heated actinomycetes. Cells that could be heat inactivated also were inactivated by amino group acetylation or protease digestion, whereas cells that were unaffected by heat were not inactivated by these treatments. Coaggregation reactions of each kind were Ca2+ dependent and insensitive to dextranase treatment. These findings are consistent with the hypothesis that human strains of A. viscosus and A. naeslundii coaggregate with strains of S. sanguis and S. mitis by a system of specific cell surface interactions between protein or glycoprotein receptors on one cell type and carbohydrates on the other type.", "contents": "Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Coaggregation reactions between actinomycete and streptococcal cells occurred frequently when human strains of Actinomyces viscosus or A. naeslundii were mixed with human isolates of Streptococcus sanguis or S. mitis, but were infrequent with other oral actinomycetes and streptococci. Two groups of actinomycetes and four groups of streptococci were defined by the patterns of their coaggregation reactions and by the ability of beta-linked galactosides (i.e., lactose) to reverse these reactions. Coaggregations occurred by one of the following three kinds to cell-cell interactions: (i) coaggregation that was blocked by heating the streptococcus but not the actinomycete and was not reversed by lactose; (ii) coaggregation that was blocked by heating the actinomycete but not the streptococcus and was reversed by lactose; and (iii) coaggregation that was blocked only by heating both cell types. The latter reaction was a combination of the first two since lactose reversed coaggregation between heated streptococci and unheated actinomycetes but did not reverse coaggregations between unheated streptococci and heated actinomycetes. Cells that could be heat inactivated also were inactivated by amino group acetylation or protease digestion, whereas cells that were unaffected by heat were not inactivated by these treatments. Coaggregation reactions of each kind were Ca2+ dependent and insensitive to dextranase treatment. These findings are consistent with the hypothesis that human strains of A. viscosus and A. naeslundii coaggregate with strains of S. sanguis and S. mitis by a system of specific cell surface interactions between protein or glycoprotein receptors on one cell type and carbohydrates on the other type."}
{"id": "PMID:468377", "title": "Phosphoenolpyruvate-dependent sucrose phosphotransferase activity in Streptococcus mutans NCTC 10449.", "content": "A phosphoenolpyruvate-dependent sucrose phosphotransferase system (PTS) has been demonstrated, by an enzyme-coupled reaction and product isolation, in decryptified cell suspensions of the cariogenic microorganism Streptococcus mutans NCTC 10449. The apparent sucrose PTS reaction for sucrose-adapted, sucrose-challenged cells displayed saturation kinetics with an apparent Km of 7.14 x 10(-5) M, which was distinct from the Km of the glucose PTS activity of glucose-adapted, glucose-challenged cells. Both the sucrose and the glucose PTS activities appear to be inducible and under separate genetic control. The sucrose PTS reaction demonstrated in decryptified cells had an absolute requirement for phosphoenolpyruvate. Only 2-phosphoglycerate, the immediate glycolytic precursor of phosphoenolpyruvate, was found to substitute for phosphoenolpyruvate in this reaction in the absence of fluoride. The sucrose PTS activity of sucrose-adapted cells was competitively inhibited by raffinose and lactose; these same sugars had no effect on the apparent glucose PTS activity. Fructose was the only carbohydrate tested other than sucrose which elicited an apparent PTS reaction in sucrose-adapted cells. The product of the sucrose PTS reaction was isolated and behaved chromatographically on a Dowex-1-X8 column like a monophosphate ester. Alkaline phosphatase treatment of the presumptive sucrose monophosphate liberated a component which behaved chromatographically like free sucrose. Subsequent acid hydrolysis of this component produced moieties which behaved chromatographically like glucose and fructose.", "contents": "Phosphoenolpyruvate-dependent sucrose phosphotransferase activity in Streptococcus mutans NCTC 10449. A phosphoenolpyruvate-dependent sucrose phosphotransferase system (PTS) has been demonstrated, by an enzyme-coupled reaction and product isolation, in decryptified cell suspensions of the cariogenic microorganism Streptococcus mutans NCTC 10449. The apparent sucrose PTS reaction for sucrose-adapted, sucrose-challenged cells displayed saturation kinetics with an apparent Km of 7.14 x 10(-5) M, which was distinct from the Km of the glucose PTS activity of glucose-adapted, glucose-challenged cells. Both the sucrose and the glucose PTS activities appear to be inducible and under separate genetic control. The sucrose PTS reaction demonstrated in decryptified cells had an absolute requirement for phosphoenolpyruvate. Only 2-phosphoglycerate, the immediate glycolytic precursor of phosphoenolpyruvate, was found to substitute for phosphoenolpyruvate in this reaction in the absence of fluoride. The sucrose PTS activity of sucrose-adapted cells was competitively inhibited by raffinose and lactose; these same sugars had no effect on the apparent glucose PTS activity. Fructose was the only carbohydrate tested other than sucrose which elicited an apparent PTS reaction in sucrose-adapted cells. The product of the sucrose PTS reaction was isolated and behaved chromatographically on a Dowex-1-X8 column like a monophosphate ester. Alkaline phosphatase treatment of the presumptive sucrose monophosphate liberated a component which behaved chromatographically like free sucrose. Subsequent acid hydrolysis of this component produced moieties which behaved chromatographically like glucose and fructose."}
{"id": "PMID:468378", "title": "Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans.", "content": "A phosphoenolpyruvate-dependent sucrose phosphotransferase system has been identified in Streptococcus mutans. Sucrose phosphotransferase activity was inducible by sucrose and had an apparent Km for sucrose of 70 microM. The product of the sucrose phosphotransferase reaction was isolated and identified as sucrose phosphate. Additional analysis revealed that the phosphate group was on the glucose moiety. Mutants unable to grow in media containing low concentrations of sucrose were isolated and found to be missing either sucrose phosphotransferase activity or the ability to hydrolyze sucrose phosphate.", "contents": "Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans. A phosphoenolpyruvate-dependent sucrose phosphotransferase system has been identified in Streptococcus mutans. Sucrose phosphotransferase activity was inducible by sucrose and had an apparent Km for sucrose of 70 microM. The product of the sucrose phosphotransferase reaction was isolated and identified as sucrose phosphate. Additional analysis revealed that the phosphate group was on the glucose moiety. Mutants unable to grow in media containing low concentrations of sucrose were isolated and found to be missing either sucrose phosphotransferase activity or the ability to hydrolyze sucrose phosphate."}
{"id": "PMID:468379", "title": "Electron microscopic studies on the attachment of Mycoplasma pneumoniae to guinea pig erythrocytes.", "content": "A mechanism of pathogenicity of Mycoplasma pneumoniae is its ability to attach to the surface of mammalian cells. It has previously been demonstrated by others that M. pneumoniae adheres with a specialized terminal structure, the \"tip,\" to ciliated epithelial cells of the respiratory tract. In this report we show by electron microscopy that M. pneumoniae adsorbs with membrane sites other than the tip to guinea pig erythrocytes.", "contents": "Electron microscopic studies on the attachment of Mycoplasma pneumoniae to guinea pig erythrocytes. A mechanism of pathogenicity of Mycoplasma pneumoniae is its ability to attach to the surface of mammalian cells. It has previously been demonstrated by others that M. pneumoniae adheres with a specialized terminal structure, the \"tip,\" to ciliated epithelial cells of the respiratory tract. In this report we show by electron microscopy that M. pneumoniae adsorbs with membrane sites other than the tip to guinea pig erythrocytes."}
{"id": "PMID:468380", "title": "Choriogonadotropin-like antigen in an anaerobic bacterium, Eubacterium lentum, isolated from a rectal tumor.", "content": "Using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of Eubacterium lenthum that was originally isolated from a rectal tumor. In contrast, both immunohistochemical reactions were negative when applied to a strain of Corynebacterium parvum and to pathogenic and nonpathogenic strains of Agrobacterium tumefaciens. Our results demonstrate for the first time the expression of the choriogonadotropin-like antigen in an obligate anaerobe and support our previous findings that the choriogonadotropin like material appears to be expressed only in \"cancer-associated bacteria\" but that not all bacteria associated with the malignant neoplasms have the capacity to express the antigen, at least in amounts detectable by immunohistochemistry.", "contents": "Choriogonadotropin-like antigen in an anaerobic bacterium, Eubacterium lentum, isolated from a rectal tumor. Using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of Eubacterium lenthum that was originally isolated from a rectal tumor. In contrast, both immunohistochemical reactions were negative when applied to a strain of Corynebacterium parvum and to pathogenic and nonpathogenic strains of Agrobacterium tumefaciens. Our results demonstrate for the first time the expression of the choriogonadotropin-like antigen in an obligate anaerobe and support our previous findings that the choriogonadotropin like material appears to be expressed only in \"cancer-associated bacteria\" but that not all bacteria associated with the malignant neoplasms have the capacity to express the antigen, at least in amounts detectable by immunohistochemistry."}
{"id": "PMID:468381", "title": "Deoxyribonucleic acid synthesis, cell cycle progression, and division of Chlamydia-infected HeLa 229 cells.", "content": "The fate of lymphogranuloma venereum strain Chlamydia-infected HeLa 229 cells was examined by determining the rate of deoxyribonucleic acid synthesis and the kinetics of entry into and progression through S phase and by time-lapse cinemicrography. At an input multiplicity of 5 or less, Chlamydia-infected cells showed no inhibition of host deoxyribonucleic acid synthesis or cell cycle progression. Cinemicrography showed division of inclusion-containing cells, with one or both daughters receiving chlamydial inclusions. Analysis of the family trees indicated that the generation times of infected HeLa 229 were not altered relative to those of the uninfected cells.", "contents": "Deoxyribonucleic acid synthesis, cell cycle progression, and division of Chlamydia-infected HeLa 229 cells. The fate of lymphogranuloma venereum strain Chlamydia-infected HeLa 229 cells was examined by determining the rate of deoxyribonucleic acid synthesis and the kinetics of entry into and progression through S phase and by time-lapse cinemicrography. At an input multiplicity of 5 or less, Chlamydia-infected cells showed no inhibition of host deoxyribonucleic acid synthesis or cell cycle progression. Cinemicrography showed division of inclusion-containing cells, with one or both daughters receiving chlamydial inclusions. Analysis of the family trees indicated that the generation times of infected HeLa 229 were not altered relative to those of the uninfected cells."}
{"id": "PMID:468382", "title": "Toxin-neutralizing effect of antibody against subtilisin-digested tetanus toxin.", "content": "A form of systemic tetanus with atypical symptoms was observed in mice injected in the left thigh with a mixture of tetanus toxin and antibody produced in guinea pigs against a fragment of toxin obtained from a subtilisin digest of the crystallized toxin. The mice did not show typical symptoms of the local tetanus such as convulsions or spastic paralysis of the injected limb.", "contents": "Toxin-neutralizing effect of antibody against subtilisin-digested tetanus toxin. A form of systemic tetanus with atypical symptoms was observed in mice injected in the left thigh with a mixture of tetanus toxin and antibody produced in guinea pigs against a fragment of toxin obtained from a subtilisin digest of the crystallized toxin. The mice did not show typical symptoms of the local tetanus such as convulsions or spastic paralysis of the injected limb."}
{"id": "PMID:468383", "title": "Antibody to Rickettsia mooseri erythrocyte-sensitizing substance.", "content": "Erythrocyte-sensitizing antigens from Rickettsia mooseri (R. typhi) were found to bind to rabbit erythrocytes. Upon reintroduction into the donor, such antigen-sensitized cells caused the production of antibodies of restricted serological reactivity.", "contents": "Antibody to Rickettsia mooseri erythrocyte-sensitizing substance. Erythrocyte-sensitizing antigens from Rickettsia mooseri (R. typhi) were found to bind to rabbit erythrocytes. Upon reintroduction into the donor, such antigen-sensitized cells caused the production of antibodies of restricted serological reactivity."}
{"id": "PMID:468384", "title": "Enhancement and suppression by actinomycin D of a Vero cell nontransmissible measles infection.", "content": "The effect of low doses of actinomycin D on Vero cell nontransmissible measles infection produced after cocultivation with a HeLa subline with persistent defective infection by Edmonston measles virus was examined by pretreatment of Vero cells with the drug and treatment at 1, 4, and 7 days after Vero cell infection. Pretreatment enhanced focal formation of viral immunofluorescent Vero cell syncytia but did not induce synthesis of detectable amounts od cocultures suppressed syncytial formation, and treatment of 4- and 7-day-old cocultures had little, if any, effect on syncytial formation. Pretreatment also eliminated a transient resistance to homologous superinfection. A possible relation of these findings to the presence of a cell-associated viral inhibitor in the persistently infected HeLa cells is discussed.", "contents": "Enhancement and suppression by actinomycin D of a Vero cell nontransmissible measles infection. The effect of low doses of actinomycin D on Vero cell nontransmissible measles infection produced after cocultivation with a HeLa subline with persistent defective infection by Edmonston measles virus was examined by pretreatment of Vero cells with the drug and treatment at 1, 4, and 7 days after Vero cell infection. Pretreatment enhanced focal formation of viral immunofluorescent Vero cell syncytia but did not induce synthesis of detectable amounts od cocultures suppressed syncytial formation, and treatment of 4- and 7-day-old cocultures had little, if any, effect on syncytial formation. Pretreatment also eliminated a transient resistance to homologous superinfection. A possible relation of these findings to the presence of a cell-associated viral inhibitor in the persistently infected HeLa cells is discussed."}
{"id": "PMID:468385", "title": "Protection of mice against Giardia muris infection.", "content": "Strains of mice showing relatively rapid (BALB/c) and defective (C3H/He) spontaneous elimination of Giardia muris displayed marked differences in the degree of resistance to infection induced by prior injection of trophozoites in Freund complete adjuvant.", "contents": "Protection of mice against Giardia muris infection. Strains of mice showing relatively rapid (BALB/c) and defective (C3H/He) spontaneous elimination of Giardia muris displayed marked differences in the degree of resistance to infection induced by prior injection of trophozoites in Freund complete adjuvant."}
{"id": "PMID:468386", "title": "Antigenic changes in Vibrio cholerae biotype eltor serotype Ogawa after bacteriophage infection.", "content": "Vibrio cholerae biotype eltor serotype Ogawa cultures infected with temperate bacteriophage from lysogens of biotype eltor serotype Hikojima yielded types with altered agglutination reactions.", "contents": "Antigenic changes in Vibrio cholerae biotype eltor serotype Ogawa after bacteriophage infection. Vibrio cholerae biotype eltor serotype Ogawa cultures infected with temperate bacteriophage from lysogens of biotype eltor serotype Hikojima yielded types with altered agglutination reactions."}
{"id": "PMID:468387", "title": "Isolation of plasmids from mycobacteria.", "content": "A procedure for preparing sodium dodecyl sulfate-salt-cleared lysates of mycobacteria was developed. Standard techniques were employed to demonstrate covalently closed circular deoxyribonucleic acid in lysates of nine strains of Mycobacterium avium-intracellulare.", "contents": "Isolation of plasmids from mycobacteria. A procedure for preparing sodium dodecyl sulfate-salt-cleared lysates of mycobacteria was developed. Standard techniques were employed to demonstrate covalently closed circular deoxyribonucleic acid in lysates of nine strains of Mycobacterium avium-intracellulare."}
{"id": "PMID:468388", "title": "Immunological and physicochemical properties of a highly purified allergen from Dirofilaria immitis.", "content": "Allergen in crude extract of Dirofilaria immitis was purified and separated from IgG-inducing antigens by a combination of DEAE-Sephadex A-50 chromatography, Sephadex G-200 gel filtration and starch gel zone electrophoresis. The purified preparation was proved to be one protein band by sodium dodecyl sulfate polyacrylamide gel (SDS-gel) electrophoresis and one precipitin arc by immunodiffusion. The molecular weight of the purified allergen was estimated to be approximately 20,000 by gel filtration and 15,000 by SDS-gel electrophoresis. The carbohydrate content of the preparation was apparently low, about 2%. The allergen was positively charged, and its determinant group was protein in nature. It was resistant to tryptic, pepsic and chymotryptic digestion, periodate oxidation and DNase and RNase digestion but very sensitive to pronase digestion. Allergen was inclined to aggregate each other in the buffered solution. It was also very resistant to vibration, heat (80 degrees C for 1 h) and acid (pH 2.5) and alkali (pH 11.0) treatments. Rats as well as mice immunized with allergen developed only a reaginic antibody and no hemagglutination antibody.", "contents": "Immunological and physicochemical properties of a highly purified allergen from Dirofilaria immitis. Allergen in crude extract of Dirofilaria immitis was purified and separated from IgG-inducing antigens by a combination of DEAE-Sephadex A-50 chromatography, Sephadex G-200 gel filtration and starch gel zone electrophoresis. The purified preparation was proved to be one protein band by sodium dodecyl sulfate polyacrylamide gel (SDS-gel) electrophoresis and one precipitin arc by immunodiffusion. The molecular weight of the purified allergen was estimated to be approximately 20,000 by gel filtration and 15,000 by SDS-gel electrophoresis. The carbohydrate content of the preparation was apparently low, about 2%. The allergen was positively charged, and its determinant group was protein in nature. It was resistant to tryptic, pepsic and chymotryptic digestion, periodate oxidation and DNase and RNase digestion but very sensitive to pronase digestion. Allergen was inclined to aggregate each other in the buffered solution. It was also very resistant to vibration, heat (80 degrees C for 1 h) and acid (pH 2.5) and alkali (pH 11.0) treatments. Rats as well as mice immunized with allergen developed only a reaginic antibody and no hemagglutination antibody."}
{"id": "PMID:468389", "title": "Evaluation of bee sting allergy by skin tests and serum antibody assays.", "content": "We studied 55 subjects who had had anaphylactic reactions to bee stings within the previous 3 years. 38 out of 54 tested had IgE antibody to honey bee venom (HBV) as measured by radioallergosorbent test (RAST). On skin testing, 30 out of 34 had a positive test to HBV. Of these, 26 had a positive RAST. A positive skin test to HBV at high dilution or else a high anti-HBV RAST score appeared to identify those who, in a 6-month follow-up period, were at risk of developing further anaphylaxis following bee stings or immunotherapy. Of the two tests, RAST appeared to be the less sensitive. Measurements of IgG antibody to phospholipase A were seldom available for the period immediately preceding an anaphylactic episode and proved to be a poor means of predicting the liability to bee sting anaphylaxis in subsequent months.", "contents": "Evaluation of bee sting allergy by skin tests and serum antibody assays. We studied 55 subjects who had had anaphylactic reactions to bee stings within the previous 3 years. 38 out of 54 tested had IgE antibody to honey bee venom (HBV) as measured by radioallergosorbent test (RAST). On skin testing, 30 out of 34 had a positive test to HBV. Of these, 26 had a positive RAST. A positive skin test to HBV at high dilution or else a high anti-HBV RAST score appeared to identify those who, in a 6-month follow-up period, were at risk of developing further anaphylaxis following bee stings or immunotherapy. Of the two tests, RAST appeared to be the less sensitive. Measurements of IgG antibody to phospholipase A were seldom available for the period immediately preceding an anaphylactic episode and proved to be a poor means of predicting the liability to bee sting anaphylaxis in subsequent months."}
{"id": "PMID:468390", "title": "Characterization of bovine leukocytes involved in antibody-dependent cell cytotoxicity.", "content": "By the use of cell surface markers combined with physical separation techniques, bovine mononuclear leukocytes active as K cells in antibody-dependent cell cytotoxicity were characterized. The most active cells were glass wool adherent and were presumed to be macrophages. In the glass wool nonadherent population, heterogeneity was detected with respect to the complement (C) receptor but all cells expressed the Fc receptor. The C receptor-positive population adhered to nylon wool but was not a B cell and was assumed to be monocyte. The C receptor-negative population, the true lymphoid K cell, was nonadherent to nylon and was suggested to be an Fc receptor-positive null lymphocyte. The results were compared to those obtained with other species.", "contents": "Characterization of bovine leukocytes involved in antibody-dependent cell cytotoxicity. By the use of cell surface markers combined with physical separation techniques, bovine mononuclear leukocytes active as K cells in antibody-dependent cell cytotoxicity were characterized. The most active cells were glass wool adherent and were presumed to be macrophages. In the glass wool nonadherent population, heterogeneity was detected with respect to the complement (C) receptor but all cells expressed the Fc receptor. The C receptor-positive population adhered to nylon wool but was not a B cell and was assumed to be monocyte. The C receptor-negative population, the true lymphoid K cell, was nonadherent to nylon and was suggested to be an Fc receptor-positive null lymphocyte. The results were compared to those obtained with other species."}
{"id": "PMID:468391", "title": "Delayed-type hypersensitivity measured as an increase of ear thickness in guinea pigs.", "content": "Determination of swelling at an intracutaneous test site in the pinna of the ear of guinea pigs immunized with protein antigens in complete Freund's adjuvant was found to be a more sensitive assay of delayed-type hypersensitivity than the measurement of flank skin erythema and induration. In fact, 100 times less specific antigen was needed to detect 24-hour reactivity in the pinna of the ear. Reactivity early after sensitization (cutaneous basophil hypersensitivity), however, was best revealed as an erythematous lesion of the flank skin 24 h after testing.", "contents": "Delayed-type hypersensitivity measured as an increase of ear thickness in guinea pigs. Determination of swelling at an intracutaneous test site in the pinna of the ear of guinea pigs immunized with protein antigens in complete Freund's adjuvant was found to be a more sensitive assay of delayed-type hypersensitivity than the measurement of flank skin erythema and induration. In fact, 100 times less specific antigen was needed to detect 24-hour reactivity in the pinna of the ear. Reactivity early after sensitization (cutaneous basophil hypersensitivity), however, was best revealed as an erythematous lesion of the flank skin 24 h after testing."}
{"id": "PMID:468396", "title": "Serum complement and protein metabolism in chronic dialysis patients.", "content": "1. Patients receiving regular hemodialysis treatment who are permitted to select their own diets are in danger of a protein deficiency manifested by a significant reduction in serum transferrin and in complement C3c. 2. Correlation analysis within the dialysis group revealed no secure connexion between the complement C3c and total hemolytic activity respectively, and transferrin levels. 3. Analysis of diets showed that protein intake and the serum transferrin level correlate. No such correlation was found for the complement C3c or total hemolytic activity. 4. Low transferrin levels in the serum appear to result in more severe anemia among dialysis patients. 5. Knowledge of the serum transferrin level permits much more exact assessment of the protein metabolism in regular hemodialysis patients than knowledge of the total hemolytic activity or the level of the complement C3c.", "contents": "Serum complement and protein metabolism in chronic dialysis patients. 1. Patients receiving regular hemodialysis treatment who are permitted to select their own diets are in danger of a protein deficiency manifested by a significant reduction in serum transferrin and in complement C3c. 2. Correlation analysis within the dialysis group revealed no secure connexion between the complement C3c and total hemolytic activity respectively, and transferrin levels. 3. Analysis of diets showed that protein intake and the serum transferrin level correlate. No such correlation was found for the complement C3c or total hemolytic activity. 4. Low transferrin levels in the serum appear to result in more severe anemia among dialysis patients. 5. Knowledge of the serum transferrin level permits much more exact assessment of the protein metabolism in regular hemodialysis patients than knowledge of the total hemolytic activity or the level of the complement C3c."}
{"id": "PMID:468398", "title": "Agarose-encapsulated adsorbents.", "content": "A method is described for the encapsulation of ion-exchange and adsorbent resins of native particle diameter 0.2-1.0 mm into agarose spheres of diameter 5-10 mm. Plasma components diffuse rapidly through the agarose coating, but blood corpuscles have no direct access to the resins. At least 0.3 g of resin can be incorporated into 1 g of the agarose beads, so that the effective surface area with regard to erythrocytes, thrombocytes, and leucocytes is reduced by a factor of at least 5, and up to 20, depending on the native resin particle size. Pulverised active charcoal, or resins in powder form can also be encapsulated in this manner. The haemocompatibility of the agarose coating seems to be considerably more acceptable than that of the adsorbents in their native state.", "contents": "Agarose-encapsulated adsorbents. A method is described for the encapsulation of ion-exchange and adsorbent resins of native particle diameter 0.2-1.0 mm into agarose spheres of diameter 5-10 mm. Plasma components diffuse rapidly through the agarose coating, but blood corpuscles have no direct access to the resins. At least 0.3 g of resin can be incorporated into 1 g of the agarose beads, so that the effective surface area with regard to erythrocytes, thrombocytes, and leucocytes is reduced by a factor of at least 5, and up to 20, depending on the native resin particle size. Pulverised active charcoal, or resins in powder form can also be encapsulated in this manner. The haemocompatibility of the agarose coating seems to be considerably more acceptable than that of the adsorbents in their native state."}
{"id": "PMID:468404", "title": "An in vitro comparison of capillary flow dialyzer performances on a single needle system (double headpump).", "content": "The ultrafiltration, clearance and priming characteristics of six capillary dialyzers (C-DAK 2.5 D, C-DAK 1.8 D, Tri-Ex 1, CF M 1500, N16F160 and HF 150) were studied in single needle dialysis by way of the double headpump (BL 760, Bellco Ltd). A good control of the ultrafiltration by an appropriate choice of the mean bloodcompartment pressures (Pbo) is present in all dialyzers. After correction of the clearance for ultrafiltration the clearances of urea, creatinine, sodiumiothalamate-l125 and cyanocobalamine-Co58 (vit. B 12) rise with increasing mean pressures in the bloodcompartment of all dialyzers. We assume that a better use of available surface area due to the characteristics of the double headpump such as TMP mainly influenced by Pbo pressures and other such as tidal flow are responsible for the excellent in vitro clearances of these dialyzers with this type of single needle system.", "contents": "An in vitro comparison of capillary flow dialyzer performances on a single needle system (double headpump). The ultrafiltration, clearance and priming characteristics of six capillary dialyzers (C-DAK 2.5 D, C-DAK 1.8 D, Tri-Ex 1, CF M 1500, N16F160 and HF 150) were studied in single needle dialysis by way of the double headpump (BL 760, Bellco Ltd). A good control of the ultrafiltration by an appropriate choice of the mean bloodcompartment pressures (Pbo) is present in all dialyzers. After correction of the clearance for ultrafiltration the clearances of urea, creatinine, sodiumiothalamate-l125 and cyanocobalamine-Co58 (vit. B 12) rise with increasing mean pressures in the bloodcompartment of all dialyzers. We assume that a better use of available surface area due to the characteristics of the double headpump such as TMP mainly influenced by Pbo pressures and other such as tidal flow are responsible for the excellent in vitro clearances of these dialyzers with this type of single needle system."}
{"id": "PMID:468405", "title": "Hemodialysis with \"adequate\" sodium concentration in dialysate.", "content": "This investigation was undertaken to define the \"adequate\" sodium concentration in the dialytic fluid allowing to maintain a stable plasma effective osmolality during dialysis. Isonatric dialysate is shown to miss this aim by inducing a predictable postdialytic hypernatremia. To avoid this effect a new approach was made. 17 clinically stabilized patients, previously dialyzed over a period of at least 2 years with a dialysate sodium concentration of 133 mEq/l, underwent dialysis with the \"adequate\" sodium concentration in the dialysate for over 3 years. During dialysis cramps, headache, hypotension, hypertensive crises and postdialytic weakness were reduced in frequency and nearly disappeared. No deterioration in blood pressure control occurred and improvement in some general parameters (hematocrit, glucose and insulin metabolism, well-being) was reported after prolonged treatment.", "contents": "Hemodialysis with \"adequate\" sodium concentration in dialysate. This investigation was undertaken to define the \"adequate\" sodium concentration in the dialytic fluid allowing to maintain a stable plasma effective osmolality during dialysis. Isonatric dialysate is shown to miss this aim by inducing a predictable postdialytic hypernatremia. To avoid this effect a new approach was made. 17 clinically stabilized patients, previously dialyzed over a period of at least 2 years with a dialysate sodium concentration of 133 mEq/l, underwent dialysis with the \"adequate\" sodium concentration in the dialysate for over 3 years. During dialysis cramps, headache, hypotension, hypertensive crises and postdialytic weakness were reduced in frequency and nearly disappeared. No deterioration in blood pressure control occurred and improvement in some general parameters (hematocrit, glucose and insulin metabolism, well-being) was reported after prolonged treatment."}
{"id": "PMID:468407", "title": "Monitoring of metabolites applying fibre-entrapped enzymes in a calorimetric system. I -- Glucose and urea determination.", "content": "A simple device, consisting of a heat sensor placed in close contact to fibres containing enzymes and connected to a temperature measuring unit has been developed. The system monitors the temperature variations due to the enzymatic reaction when substrate solutions flow through the measuring cell. Fibres containing the enzymes glucose oxidase and catalase for the determination of glucose and fibres containing urease for the determination of urea were tested. A linear relationship between the substrate concentration and the deltaT recorded was obtained in both cases.", "contents": "Monitoring of metabolites applying fibre-entrapped enzymes in a calorimetric system. I -- Glucose and urea determination. A simple device, consisting of a heat sensor placed in close contact to fibres containing enzymes and connected to a temperature measuring unit has been developed. The system monitors the temperature variations due to the enzymatic reaction when substrate solutions flow through the measuring cell. Fibres containing the enzymes glucose oxidase and catalase for the determination of glucose and fibres containing urease for the determination of urea were tested. A linear relationship between the substrate concentration and the deltaT recorded was obtained in both cases."}
{"id": "PMID:468410", "title": "Increased monocyte-mediated cytostasis of lymphoid cell lines in breast and lung cancer patients.", "content": "Peripheral blood mononuclear cells of most normal adults and patients with breast or lung cancer were found to inhibit [3H] thymidine uptake by lymphoid cell lines in a growth inhibition assay. At effector:target cell ratios between 5:1 and 20:1, lung cancer patients and breast cancer patients, when compared to normal individuals, demonstrated significantly greater inhibition of [3H] thymidine uptake by a human lymphoid cell line (F-265). The effector cells were adherent and were probably monocytes. Sephadex G-10 column passage or adherence to plastic removed most growth-inhibitory activity. Adherent cells recovered from plastic flasks (88-94% monocytes) were strongly growth-inhibitory. Lung cancer patients receiving BCG immunotherapy were found to have an apparently increased activity compared to patients not treated with BCG. The possible mediation of the growth inhibition by release of prostaglandins was suggested by the reduced cytostatic effects in the presence of indomethacin. Growth-inhibitory activity was not species-restricted, since human effector cells and also effector cells from tumor-bearing mice were reactive against the human target cell and against a murine lymphoma line (RBL-5). Natural killer (NK) cells did not appear to contribute appreciably to the observed cytostasis, since the levels of their activities did not correlate, and human NK cells are non-adherent and have little reactivity against F-265 or RBL-5. The inhibition of [3H]thymidine uptake by target cells was demonstrated to be a good reflection of actual inhibition of proliferation, since incubation of adherent cells from cancer patients with F-265 resulted in similar degrees of reduction in the number of target cells and in [3H] thymidine uptake.", "contents": "Increased monocyte-mediated cytostasis of lymphoid cell lines in breast and lung cancer patients. Peripheral blood mononuclear cells of most normal adults and patients with breast or lung cancer were found to inhibit [3H] thymidine uptake by lymphoid cell lines in a growth inhibition assay. At effector:target cell ratios between 5:1 and 20:1, lung cancer patients and breast cancer patients, when compared to normal individuals, demonstrated significantly greater inhibition of [3H] thymidine uptake by a human lymphoid cell line (F-265). The effector cells were adherent and were probably monocytes. Sephadex G-10 column passage or adherence to plastic removed most growth-inhibitory activity. Adherent cells recovered from plastic flasks (88-94% monocytes) were strongly growth-inhibitory. Lung cancer patients receiving BCG immunotherapy were found to have an apparently increased activity compared to patients not treated with BCG. The possible mediation of the growth inhibition by release of prostaglandins was suggested by the reduced cytostatic effects in the presence of indomethacin. Growth-inhibitory activity was not species-restricted, since human effector cells and also effector cells from tumor-bearing mice were reactive against the human target cell and against a murine lymphoma line (RBL-5). Natural killer (NK) cells did not appear to contribute appreciably to the observed cytostasis, since the levels of their activities did not correlate, and human NK cells are non-adherent and have little reactivity against F-265 or RBL-5. The inhibition of [3H]thymidine uptake by target cells was demonstrated to be a good reflection of actual inhibition of proliferation, since incubation of adherent cells from cancer patients with F-265 resulted in similar degrees of reduction in the number of target cells and in [3H] thymidine uptake."}
{"id": "PMID:468411", "title": "HLA antigens in familial Hodgkin's disease.", "content": "In a study of 13 families prone to Hodgkin's disease, the probands showed significant excesses of the HLA antigens Bw35 (7 cases) and Bw37 (3 cases). Although based on a small number of patients, the results suggest that immunogenetic mechanisms account at least partly for the familial predisposition to Hodgkin's disease.", "contents": "HLA antigens in familial Hodgkin's disease. In a study of 13 families prone to Hodgkin's disease, the probands showed significant excesses of the HLA antigens Bw35 (7 cases) and Bw37 (3 cases). Although based on a small number of patients, the results suggest that immunogenetic mechanisms account at least partly for the familial predisposition to Hodgkin's disease."}
{"id": "PMID:468412", "title": "Demonstration of placental alkaline phosphatase in human breast cancer.", "content": "The screening of a series of 11 metastatic breast tumors for the presence of the placental isoenzyme of alkaline phosphatase (EC3.1.3.1) by RIA revealed one strong producer. The alkaline phosphatase of this tumor was characterized with respect to its immunochemical cross-reactivity, inhibition by L-phenylalanine and levamisole, subunit molecular weight (Mr) and isoelectric point (pl) in two-dimensional electrophoresis, and one-dimensional peptide map. In all parameters of the characterization, the tumor alkaline phosphatase, except for subunit molecular weight which was slightly lower (60,000 versus 64,000 for the placental isoenzyme). No strong placental alkaline phosphatase producers were found among 16 primary tumors examined by RIA. The screening of patients' sera for the placental alkaline phosphatase using RIA indicated elevated levels over post-menopausal controls in 20% of the metastatic patients. Only 3% of the primary patients had elevated serum levels. These results suggest that the placental isoenzyme of alkaline phosphatase may be a useful tumor marker for recurrent breast cancer.", "contents": "Demonstration of placental alkaline phosphatase in human breast cancer. The screening of a series of 11 metastatic breast tumors for the presence of the placental isoenzyme of alkaline phosphatase (EC3.1.3.1) by RIA revealed one strong producer. The alkaline phosphatase of this tumor was characterized with respect to its immunochemical cross-reactivity, inhibition by L-phenylalanine and levamisole, subunit molecular weight (Mr) and isoelectric point (pl) in two-dimensional electrophoresis, and one-dimensional peptide map. In all parameters of the characterization, the tumor alkaline phosphatase, except for subunit molecular weight which was slightly lower (60,000 versus 64,000 for the placental isoenzyme). No strong placental alkaline phosphatase producers were found among 16 primary tumors examined by RIA. The screening of patients' sera for the placental alkaline phosphatase using RIA indicated elevated levels over post-menopausal controls in 20% of the metastatic patients. Only 3% of the primary patients had elevated serum levels. These results suggest that the placental isoenzyme of alkaline phosphatase may be a useful tumor marker for recurrent breast cancer."}
{"id": "PMID:468413", "title": "The effect of long-term food restriction on tumours in rodents.", "content": "The effect of restricting food intake by approximately 20% was studied in rats and mice. Both species were fed as similar \"natural\" diet composed chiefly of cereals and bone meal. The duration of the rat study was 24 months, but mice were studied for their whole lifespan of 36 months. In the mice, restriction increased longevity, and in both species the incidence of tumours was reduced and the onset delayed. The effect upon the different types of tumour varied; some types appeared to be unaffected by restriction, but the most common types in each species, liver tumours in mice, pituitary, mammary and skin tumours in rats, were significantly reduced.", "contents": "The effect of long-term food restriction on tumours in rodents. The effect of restricting food intake by approximately 20% was studied in rats and mice. Both species were fed as similar \"natural\" diet composed chiefly of cereals and bone meal. The duration of the rat study was 24 months, but mice were studied for their whole lifespan of 36 months. In the mice, restriction increased longevity, and in both species the incidence of tumours was reduced and the onset delayed. The effect upon the different types of tumour varied; some types appeared to be unaffected by restriction, but the most common types in each species, liver tumours in mice, pituitary, mammary and skin tumours in rats, were significantly reduced."}
{"id": "PMID:468414", "title": "Liver-colonizing melanoma cells selected from B-16 melanoma.", "content": "Liver-colonizing tumor cells were selected from B-16 melanoma by a stepwise procedure: (1) injection of the original cells through the portal circulation: (2) removal of tumor cells from the hepatic tumor nodules for culture in vitro; (3) reinjection of such cells through the portal circulation and repetition of steps 2 and 3 eight times. The predilection of these cells to form tumors in the liver was clearly demonstrated following injection of the cells intra-arterially. The accuracy of the injection technique was controlled by monitoring amonts of injected radioactively labelled Sephadex microspheres. This liver-colonizing preference was not exclusive, but relative.", "contents": "Liver-colonizing melanoma cells selected from B-16 melanoma. Liver-colonizing tumor cells were selected from B-16 melanoma by a stepwise procedure: (1) injection of the original cells through the portal circulation: (2) removal of tumor cells from the hepatic tumor nodules for culture in vitro; (3) reinjection of such cells through the portal circulation and repetition of steps 2 and 3 eight times. The predilection of these cells to form tumors in the liver was clearly demonstrated following injection of the cells intra-arterially. The accuracy of the injection technique was controlled by monitoring amonts of injected radioactively labelled Sephadex microspheres. This liver-colonizing preference was not exclusive, but relative."}
{"id": "PMID:468415", "title": "Milky spots (Taches laiteuses) as structures which trap asbestos in mesothelial layers and their significance in the pathogenesis of mesothelial neoplasia.", "content": "The UICC standard sample of asbestos, crocidolite, was injected subcutaneously into the flanks of CBA/lac mice. Asbestos fibres were found in milky spots in the serosal cavities more than 442 days after the injection, in the form of either naked fibres or asbestos bodies. Milky spots were the only structure in which asbestos fibres were observed in the mesothelial layer. The fact that asbestos fibres were found in the liver, spleen, kidney, brain, etc., in addition to milky spots, suggested that they were transported by the blood stream throughout the body. This assumption was confirmed by the similar distribution of asbestos after intravenous administration. The vascular and cellular structure of milky spots is such that they are particularly likely to trap blood-borne asbestos fibres. Hyperplastic changes were observed in milky spots after both subcutaneous and intravenous administration of asbestos in mice. The possible involvement of milky spots in the genesis of tumors of the mesothelium following exposure of animals to asbestos is discussed.", "contents": "Milky spots (Taches laiteuses) as structures which trap asbestos in mesothelial layers and their significance in the pathogenesis of mesothelial neoplasia. The UICC standard sample of asbestos, crocidolite, was injected subcutaneously into the flanks of CBA/lac mice. Asbestos fibres were found in milky spots in the serosal cavities more than 442 days after the injection, in the form of either naked fibres or asbestos bodies. Milky spots were the only structure in which asbestos fibres were observed in the mesothelial layer. The fact that asbestos fibres were found in the liver, spleen, kidney, brain, etc., in addition to milky spots, suggested that they were transported by the blood stream throughout the body. This assumption was confirmed by the similar distribution of asbestos after intravenous administration. The vascular and cellular structure of milky spots is such that they are particularly likely to trap blood-borne asbestos fibres. Hyperplastic changes were observed in milky spots after both subcutaneous and intravenous administration of asbestos in mice. The possible involvement of milky spots in the genesis of tumors of the mesothelium following exposure of animals to asbestos is discussed."}
{"id": "PMID:468416", "title": "A heroin addiction scale for the MMPI: effectiveness in differential diagnosis in a psychiatric setting.", "content": "This study examined the discriminative utility of the Cavior Heroin Addiction Scale (HE) in separating samples of male heroin addict, alcoholic, and polydrug abuser samples from psychiatric control samples matched on sex, age, race, and education level. Heroin addicts were the only substance abuser samples that did not differentiate from its control sample. Heroin addicts obtained higher mean HE values than did the alcoholics although the polydrug abusers obtained higher mean HE values than both alcoholics and heroin addicts. HE was found to be less successful in separating these samples than was the MacAndrew Alcoholism Scale. This poorer performance is related to HE's confounding systematic relation to subject age and race, or to differences in scale construction methodology.", "contents": "A heroin addiction scale for the MMPI: effectiveness in differential diagnosis in a psychiatric setting. This study examined the discriminative utility of the Cavior Heroin Addiction Scale (HE) in separating samples of male heroin addict, alcoholic, and polydrug abuser samples from psychiatric control samples matched on sex, age, race, and education level. Heroin addicts were the only substance abuser samples that did not differentiate from its control sample. Heroin addicts obtained higher mean HE values than did the alcoholics although the polydrug abusers obtained higher mean HE values than both alcoholics and heroin addicts. HE was found to be less successful in separating these samples than was the MacAndrew Alcoholism Scale. This poorer performance is related to HE's confounding systematic relation to subject age and race, or to differences in scale construction methodology."}
{"id": "PMID:468417", "title": "Alcohol and narcotic abuse: variations on an addictive theme.", "content": "A treatment system developed to treat individuals abusing both narcotics and alcohol is described. The primary goal of treatment is change in the alcohol/drug abuse life-style which is seen as the major obstacle to successful treatment.", "contents": "Alcohol and narcotic abuse: variations on an addictive theme. A treatment system developed to treat individuals abusing both narcotics and alcohol is described. The primary goal of treatment is change in the alcohol/drug abuse life-style which is seen as the major obstacle to successful treatment."}
{"id": "PMID:468418", "title": "The influence of perceived speaker characteristics on student assessments of a drug education talk.", "content": "The relationship between student perceptions of the presenters of a drug talk and their assessments of its impact was examined with data gathered in a drug education experiment. In support of attribution theory, the results showed aspects of the speakers' appearance and demeanor to be associated with the youths' evaluation of the presentation.", "contents": "The influence of perceived speaker characteristics on student assessments of a drug education talk. The relationship between student perceptions of the presenters of a drug talk and their assessments of its impact was examined with data gathered in a drug education experiment. In support of attribution theory, the results showed aspects of the speakers' appearance and demeanor to be associated with the youths' evaluation of the presentation."}
{"id": "PMID:468419", "title": "Caffeine intake and potential effect on health of a segment of northern Canadian indigenous people.", "content": "There are strong indications that the caffeine intake of Canadian Northern Indigenous People is very substantial. Tea is the main contributor. Preliminary calculations show that daily ingestion of caffeine among these groups is much higher than ingestion levels known to have adverse medical and behavioral effects. Of particular concern is the interaction of caffeine and carbohydrate metabolism, already known to be under stress due to increases in sugar and carbohydrate intake. Symptoms characteristic of unduly high intake of caffeine are also found to a large extent in hypoglycemia, mercury intoxication, and various nutritional deficiencies. There is a need for a substantial research effort to further delineate the caffeine problem and to develop alternate beverages containing reduced amounts of caffeine.", "contents": "Caffeine intake and potential effect on health of a segment of northern Canadian indigenous people. There are strong indications that the caffeine intake of Canadian Northern Indigenous People is very substantial. Tea is the main contributor. Preliminary calculations show that daily ingestion of caffeine among these groups is much higher than ingestion levels known to have adverse medical and behavioral effects. Of particular concern is the interaction of caffeine and carbohydrate metabolism, already known to be under stress due to increases in sugar and carbohydrate intake. Symptoms characteristic of unduly high intake of caffeine are also found to a large extent in hypoglycemia, mercury intoxication, and various nutritional deficiencies. There is a need for a substantial research effort to further delineate the caffeine problem and to develop alternate beverages containing reduced amounts of caffeine."}
{"id": "PMID:468420", "title": "Drug treatment outcomes: is sex a factor?", "content": "A small random sample of data from the National CODAP system is used to determine and explain differences in treatment outcomes for men and women. Only small percentage differences, discerned through examination of categorical relationships between males and females, are found to exist. The authors conclude that this finding may suggest more positive outcomes for women in treatment since women experience more social prejudices than their male counterparts, and encounter greater barriers in completing treatment.", "contents": "Drug treatment outcomes: is sex a factor? A small random sample of data from the National CODAP system is used to determine and explain differences in treatment outcomes for men and women. Only small percentage differences, discerned through examination of categorical relationships between males and females, are found to exist. The authors conclude that this finding may suggest more positive outcomes for women in treatment since women experience more social prejudices than their male counterparts, and encounter greater barriers in completing treatment."}
{"id": "PMID:468421", "title": "Dimensions of the subjective marijuana experience.", "content": "A Drug History Questionnaire and a Marihuana Effects Questionnaire were completed by 91 male volunteers who were experienced marijuana smokers. A factor analysis was performed on the frequency of occurrence data for the Marihuana Effects Questionnaire. The resultant factors were similar to those reported previously in the literature supporting the existence of a stable, verbally definable marijuana experience. In comparison to the drug history variables of marijuana smokers in the late 1960s, our population showed increased multiple drug use, an earlier age of introduction to cannabis, and heavier use of cannabis. An analysis of the interaction of drug history variables with experienced marijuana effects suggested that the more frequently one uses cannabis, the less pronounced the experienced effects tend to be.", "contents": "Dimensions of the subjective marijuana experience. A Drug History Questionnaire and a Marihuana Effects Questionnaire were completed by 91 male volunteers who were experienced marijuana smokers. A factor analysis was performed on the frequency of occurrence data for the Marihuana Effects Questionnaire. The resultant factors were similar to those reported previously in the literature supporting the existence of a stable, verbally definable marijuana experience. In comparison to the drug history variables of marijuana smokers in the late 1960s, our population showed increased multiple drug use, an earlier age of introduction to cannabis, and heavier use of cannabis. An analysis of the interaction of drug history variables with experienced marijuana effects suggested that the more frequently one uses cannabis, the less pronounced the experienced effects tend to be."}
{"id": "PMID:468423", "title": "Attitudinal issues in methadone maintenance programs.", "content": "When the addict fails to meet treatment goals, staff may split into \"help\" vs \"shape up or be expelled\" groups. To diminish this conflict, team meeting aid the staff to better understand the addict and their own intrapsychic and interpersonal attitudes, set reasonable treatment goals, and provide concrete help. General staff meetings clarify policy and increase awareness of various role stresses. Nondegree counselor subgroups help to define stresses in this unique role.", "contents": "Attitudinal issues in methadone maintenance programs. When the addict fails to meet treatment goals, staff may split into \"help\" vs \"shape up or be expelled\" groups. To diminish this conflict, team meeting aid the staff to better understand the addict and their own intrapsychic and interpersonal attitudes, set reasonable treatment goals, and provide concrete help. General staff meetings clarify policy and increase awareness of various role stresses. Nondegree counselor subgroups help to define stresses in this unique role."}
{"id": "PMID:468424", "title": "The therapeutic community and methadone: a way of achieving abstinence.", "content": "The combination of a therapeutic community (TC) and gradual methadone detoxification of 3 to 4 months to treat long-term methadone patients is described. Most patients have done poorly on methadone and come only after serious drug and alcohol abuse or criminal behavior. An individualized treatment plan is implemented which includes individual, group, and family therapy; psychotropic drugs; blind methadone detoxification, and the techniques used in contemporary TC's. Of 215 admissions, 44% have been fully detoxified from methadone over an average of 3 1/2 months (94 patients). Of 94 patients detoxified, 20 (21%) returned to methadone treatment elsewhere and 15 (16%) stayed on such treatment.", "contents": "The therapeutic community and methadone: a way of achieving abstinence. The combination of a therapeutic community (TC) and gradual methadone detoxification of 3 to 4 months to treat long-term methadone patients is described. Most patients have done poorly on methadone and come only after serious drug and alcohol abuse or criminal behavior. An individualized treatment plan is implemented which includes individual, group, and family therapy; psychotropic drugs; blind methadone detoxification, and the techniques used in contemporary TC's. Of 215 admissions, 44% have been fully detoxified from methadone over an average of 3 1/2 months (94 patients). Of 94 patients detoxified, 20 (21%) returned to methadone treatment elsewhere and 15 (16%) stayed on such treatment."}
{"id": "PMID:468425", "title": "The relationship between treatment modality, demographic characteristics, and staff perceptions concerning their jobs in 26 Philadelphia drug treatment centers.", "content": "The staffs of 26 Philadelphia drug treatment centers, representing four major treatment modalities, were interviewed concerning their attitudes and perceptions of treatment and the treatment milieu. By employing canonical correlation, two basic modality contrasts were found that significantly differentiated these attitudes and perceptions: (1) staff in small therapeutic communities tended to manifest more positive attitudes than their counterparts in large methadone maintenance settings, and (2) outpatient drug-free staff tended to be more satisfied and optimistic than detoxification staff.", "contents": "The relationship between treatment modality, demographic characteristics, and staff perceptions concerning their jobs in 26 Philadelphia drug treatment centers. The staffs of 26 Philadelphia drug treatment centers, representing four major treatment modalities, were interviewed concerning their attitudes and perceptions of treatment and the treatment milieu. By employing canonical correlation, two basic modality contrasts were found that significantly differentiated these attitudes and perceptions: (1) staff in small therapeutic communities tended to manifest more positive attitudes than their counterparts in large methadone maintenance settings, and (2) outpatient drug-free staff tended to be more satisfied and optimistic than detoxification staff."}
{"id": "PMID:468426", "title": "Two methods of predicting drug-taking.", "content": "A behavioral analysis technique was compared to detoxified heroin addicts in their ability to predict drug behavior. It was found that the behavioral analysis technique was more successful than the heroin addicts in predicting drug-taking. Moreover, this difference was more pronounced when only transition drug usage (a drug-use week preceded by one or more drug-free weeks) was considered. The use of the behavioral analysis technique, as part of an intervention system, was discussed.", "contents": "Two methods of predicting drug-taking. A behavioral analysis technique was compared to detoxified heroin addicts in their ability to predict drug behavior. It was found that the behavioral analysis technique was more successful than the heroin addicts in predicting drug-taking. Moreover, this difference was more pronounced when only transition drug usage (a drug-use week preceded by one or more drug-free weeks) was considered. The use of the behavioral analysis technique, as part of an intervention system, was discussed."}
{"id": "PMID:468427", "title": "A survey of students' awareness of and attitudes toward drug abuse prevention programs in New York State, winter 1974/75.", "content": "The present paper reports some results from a winter 1974-1975, New York State survey of junior and senior public high school youths' awareness of and attitudes toward the drug prevention efforts their schools had undertaken. The findings indicate that most youths are not aware of community resources available for assistance with a drug problem. Substantial discrepancies were found between student ratings of the effectiveness of prevention programs and the programs which they were most frequently exposed. Finally, the data highlight the peer context and drug culture aspect of the youths' substance taking, emphasizing that the sociocultural backgrounds of students need to be taken into account in prevention program development.", "contents": "A survey of students' awareness of and attitudes toward drug abuse prevention programs in New York State, winter 1974/75. The present paper reports some results from a winter 1974-1975, New York State survey of junior and senior public high school youths' awareness of and attitudes toward the drug prevention efforts their schools had undertaken. The findings indicate that most youths are not aware of community resources available for assistance with a drug problem. Substantial discrepancies were found between student ratings of the effectiveness of prevention programs and the programs which they were most frequently exposed. Finally, the data highlight the peer context and drug culture aspect of the youths' substance taking, emphasizing that the sociocultural backgrounds of students need to be taken into account in prevention program development."}
{"id": "PMID:468428", "title": "Weight gain and edema on methadone maintenance therapy.", "content": "Methadone HCl is currently legally dispensed to approximately 80,000 heroin addicts in the United States. It is widely heralded as being virtually free from other than narcotic side effects. This report describes in detail three patients with massive fluid gain in a population of 420 addicts treated at the Hope Center methadone program in Tucson, Arizona. In no patient was there a prior history of edema, and an intensive search failed to reveal a cause for the swelling. Weight gain uniformly began 3 to 6 months after initiation of methadone or a sharp increase in the dosage, and in all three patients during periods when they sharply reduced or discontinued methadone, edema was resolved associated with a concomitant loss of weight.", "contents": "Weight gain and edema on methadone maintenance therapy. Methadone HCl is currently legally dispensed to approximately 80,000 heroin addicts in the United States. It is widely heralded as being virtually free from other than narcotic side effects. This report describes in detail three patients with massive fluid gain in a population of 420 addicts treated at the Hope Center methadone program in Tucson, Arizona. In no patient was there a prior history of edema, and an intensive search failed to reveal a cause for the swelling. Weight gain uniformly began 3 to 6 months after initiation of methadone or a sharp increase in the dosage, and in all three patients during periods when they sharply reduced or discontinued methadone, edema was resolved associated with a concomitant loss of weight."}
{"id": "PMID:468429", "title": "The epidemiology of drug use during pregnancy.", "content": "It is posited that drug-taking behaviors related to pregnancy are socially conditioned patterns and are subject to reeducation. Drug-taking behaviors of pregnant women are reviewed. It is uniformly found that multiple drug agents are consumed by the average pregnant woman during the gestation period. The unique vulnerability of the fetus and the teratogenic potential of numerous drugs are discussed. Recommendations are offered for the judicious use of drugs during pregnancy.", "contents": "The epidemiology of drug use during pregnancy. It is posited that drug-taking behaviors related to pregnancy are socially conditioned patterns and are subject to reeducation. Drug-taking behaviors of pregnant women are reviewed. It is uniformly found that multiple drug agents are consumed by the average pregnant woman during the gestation period. The unique vulnerability of the fetus and the teratogenic potential of numerous drugs are discussed. Recommendations are offered for the judicious use of drugs during pregnancy."}
{"id": "PMID:468430", "title": "Drug overdoses: a comparison of drug-dependent and nondependent individuals attending London casualty departments.", "content": "During a 1-month prospective study of patients presenting to 62 London Casualty Departments with drug-related problems, 395 drug-dependent individuals were identified. A comparison of these drug-dependent and nondependent patients attending casualty during the same period with the same presenting problem of drug overdose showed statistically significant differences in demographic characteristics and patterns of drug use between the two groups. The importance of identifying previously unrecognized drug-dependent individuals during their casualty attendance is discussed.", "contents": "Drug overdoses: a comparison of drug-dependent and nondependent individuals attending London casualty departments. During a 1-month prospective study of patients presenting to 62 London Casualty Departments with drug-related problems, 395 drug-dependent individuals were identified. A comparison of these drug-dependent and nondependent patients attending casualty during the same period with the same presenting problem of drug overdose showed statistically significant differences in demographic characteristics and patterns of drug use between the two groups. The importance of identifying previously unrecognized drug-dependent individuals during their casualty attendance is discussed."}
{"id": "PMID:468431", "title": "Methadone programs and crime reduction: a comparison of New York and California addicts.", "content": "Methadone patients in Santa Clara County (California) and Brooklyn (New York)--Addiction Research and Treatment Corporation (ARTC)--were evaluated to determine the extent of reductions in individual and community-wide criminal activity. Findings showed an overall decline in official (recorded) arrests for Brooklyn patients in a 3-year follow-up period. California patients showed similar declines in a 2-year follow-up period. Assault rates for both populations showed less of a decline. It was found that Brooklyn patients were arrested less frequently but for more severe offenses across preaddiction, addiction, and post-program entry periods. Criminal complaints to the police in the ARTC area were not reduced as compared to surrounding precincts. Individual and program performance characteristics were examined for Brooklyn patients in relation to reductions in criminal activity. A better preprogram drug history and decreased drug use while on the program were related significantly to decreased criminal activity for male and female patients, and older patients had slightly better outcomes. It was concluded that future programs should utilize more aggressive outreach techniques, including epidemiologic methods, to reach younger addicts, and that stable programs relying on central screening and a range of services would be required for success with them.", "contents": "Methadone programs and crime reduction: a comparison of New York and California addicts. Methadone patients in Santa Clara County (California) and Brooklyn (New York)--Addiction Research and Treatment Corporation (ARTC)--were evaluated to determine the extent of reductions in individual and community-wide criminal activity. Findings showed an overall decline in official (recorded) arrests for Brooklyn patients in a 3-year follow-up period. California patients showed similar declines in a 2-year follow-up period. Assault rates for both populations showed less of a decline. It was found that Brooklyn patients were arrested less frequently but for more severe offenses across preaddiction, addiction, and post-program entry periods. Criminal complaints to the police in the ARTC area were not reduced as compared to surrounding precincts. Individual and program performance characteristics were examined for Brooklyn patients in relation to reductions in criminal activity. A better preprogram drug history and decreased drug use while on the program were related significantly to decreased criminal activity for male and female patients, and older patients had slightly better outcomes. It was concluded that future programs should utilize more aggressive outreach techniques, including epidemiologic methods, to reach younger addicts, and that stable programs relying on central screening and a range of services would be required for success with them."}
{"id": "PMID:468432", "title": "Drug contagion in Jerusalem: a pilot investigation of the Israeli drug use scene.", "content": "Thirty-nine male and female adult Jewish and Arab street drug users from Jerusalem, currently in treatment, were interviewed to explore various factors associated with their own initiation into drug use and their initiation of others. These factors included the source and context of initial drug use, first drug used, physical and psychological drug reactions, and the types of gains derived from turning others on. The most significant findings from this ministudy are that the majority of drug users did not initiate anyone else into drug use, and that those who did reported a variety of nonmonetary gains.", "contents": "Drug contagion in Jerusalem: a pilot investigation of the Israeli drug use scene. Thirty-nine male and female adult Jewish and Arab street drug users from Jerusalem, currently in treatment, were interviewed to explore various factors associated with their own initiation into drug use and their initiation of others. These factors included the source and context of initial drug use, first drug used, physical and psychological drug reactions, and the types of gains derived from turning others on. The most significant findings from this ministudy are that the majority of drug users did not initiate anyone else into drug use, and that those who did reported a variety of nonmonetary gains."}
{"id": "PMID:468433", "title": "Group psychotherapy for substance abuse patients: development of a technique.", "content": "A voluntary open psychotherapy group in a Veterans Drug Dependence Treatment Center was conducted along psychodynamic-integrative lines. Interpretations were first made as with psychiatric patients. These patients regressed to drug use or dropped out. The style was changed to emphasize pertinent general questions. The derivatives of personality problems emerged in the discussions. \"Samplers\" (patients attending less than five times) and \"regulars\" (patients attending more than five times) of 104 sessions are compared with one another and the total Center population. Reviewing the literature confirmed our impression that this group treatment was worthwhile.", "contents": "Group psychotherapy for substance abuse patients: development of a technique. A voluntary open psychotherapy group in a Veterans Drug Dependence Treatment Center was conducted along psychodynamic-integrative lines. Interpretations were first made as with psychiatric patients. These patients regressed to drug use or dropped out. The style was changed to emphasize pertinent general questions. The derivatives of personality problems emerged in the discussions. \"Samplers\" (patients attending less than five times) and \"regulars\" (patients attending more than five times) of 104 sessions are compared with one another and the total Center population. Reviewing the literature confirmed our impression that this group treatment was worthwhile."}
{"id": "PMID:468434", "title": "Deterrence of methaqualone abuse through mass urinalysis.", "content": "A temporary court-ordered cessation of urine testing in Europe and the widespread abuse of a detectable methaqualone-containing drug provided an opportunity with the resumption of testing for the evaluation of the possible effects of urinalysis on usage patterns. Evidence is presented suggesting that in this instance urinalysis acted to deter usage of this particular drug.", "contents": "Deterrence of methaqualone abuse through mass urinalysis. A temporary court-ordered cessation of urine testing in Europe and the widespread abuse of a detectable methaqualone-containing drug provided an opportunity with the resumption of testing for the evaluation of the possible effects of urinalysis on usage patterns. Evidence is presented suggesting that in this instance urinalysis acted to deter usage of this particular drug."}
{"id": "PMID:468435", "title": "Swedish socialism and big business.", "content": "It is argued that during 44 years of Social Democratic government in Sweden intimate cooperation has developed between this government and multinational corporations. This cooperation was based upon the development of common interests, and was the result of an economic policy of structural rationalization and state intervention. A strong tendency of concentration of capital can be observed. Data are presented to support the theses developed.", "contents": "Swedish socialism and big business. It is argued that during 44 years of Social Democratic government in Sweden intimate cooperation has developed between this government and multinational corporations. This cooperation was based upon the development of common interests, and was the result of an economic policy of structural rationalization and state intervention. A strong tendency of concentration of capital can be observed. Data are presented to support the theses developed."}
{"id": "PMID:468436", "title": "The public hospital and its local ecology in the United States: some relationships between the \"plight of the public hospital\" and the \"plight of the cities\".", "content": "The \"plight\" of the public hospital in the United States is examined from the point of view of its relationship to the \"plight\" of the cities. Fifty-five large cities are examined and for 38 of these cities that have such hospitals the relationships of levels of public hospital services to local demographic, government fiscal, and tax structure conditions are analyzed. The principal findings point to the existence of some strong relationships and the implications for public policy of these associations are discussed.", "contents": "The public hospital and its local ecology in the United States: some relationships between the \"plight of the public hospital\" and the \"plight of the cities\". The \"plight\" of the public hospital in the United States is examined from the point of view of its relationship to the \"plight\" of the cities. Fifty-five large cities are examined and for 38 of these cities that have such hospitals the relationships of levels of public hospital services to local demographic, government fiscal, and tax structure conditions are analyzed. The principal findings point to the existence of some strong relationships and the implications for public policy of these associations are discussed."}
{"id": "PMID:468437", "title": "Homes or hospitals? Contradictions of the urban crisis.", "content": "Medical expansion is threatening to eliminate many urban residential areas, despite criticisim that argues for comprehensive planning, reduced costs, less concentrated power in the health sector, and a reversal of \"medicalization.\" Our research on expansion, which grew partly from personal participation in a local struggle against expanding institutions in Boston, revealed certain tensions in combining sociomedical research with concrete political practice. From events in Boston and from an exploratory review of periodicals, we recognized that medical expansion and community conflict occur frequently in cities throughtout the United States. Based on general theoretical perspectives from organizational analysis and political economy, we made several hypotheses that we tested through a questionnaire sent to all hospitals in the 20 largest cities of the United States and through other data available on the same hospitals. In large part, this empirical study confirmed our theoretical expectations that (a) larger medical centers show a greater tendency toward territorial growth than smaller hospitals; (b) bureaucratic and administrative dynamics lead to facilities that do not necessarily enhance patient care; (c) despite short-term cycles of expansion and contraction in public hospital growth, expansion projects are widespread and generate considerable political conflict; (d) because of the state's contradictory roles in regulation and social capital expenditures, opposition to medical expansion comes more from community organizations than from governmental monitoring or planning bodies; and (e) the needs of capital determine that medical expansion has a more detrimental impact on housing than on commercial or industrial facilities. Future expansion of private medical facilities is more likely than that of public facilities, although much private expansion may receive public subsidization. As ideologic patterns are demystified, the contradictions between medical expansion and housing needs can provide a focus for successful community organizing.", "contents": "Homes or hospitals? Contradictions of the urban crisis. Medical expansion is threatening to eliminate many urban residential areas, despite criticisim that argues for comprehensive planning, reduced costs, less concentrated power in the health sector, and a reversal of \"medicalization.\" Our research on expansion, which grew partly from personal participation in a local struggle against expanding institutions in Boston, revealed certain tensions in combining sociomedical research with concrete political practice. From events in Boston and from an exploratory review of periodicals, we recognized that medical expansion and community conflict occur frequently in cities throughtout the United States. Based on general theoretical perspectives from organizational analysis and political economy, we made several hypotheses that we tested through a questionnaire sent to all hospitals in the 20 largest cities of the United States and through other data available on the same hospitals. In large part, this empirical study confirmed our theoretical expectations that (a) larger medical centers show a greater tendency toward territorial growth than smaller hospitals; (b) bureaucratic and administrative dynamics lead to facilities that do not necessarily enhance patient care; (c) despite short-term cycles of expansion and contraction in public hospital growth, expansion projects are widespread and generate considerable political conflict; (d) because of the state's contradictory roles in regulation and social capital expenditures, opposition to medical expansion comes more from community organizations than from governmental monitoring or planning bodies; and (e) the needs of capital determine that medical expansion has a more detrimental impact on housing than on commercial or industrial facilities. Future expansion of private medical facilities is more likely than that of public facilities, although much private expansion may receive public subsidization. As ideologic patterns are demystified, the contradictions between medical expansion and housing needs can provide a focus for successful community organizing."}
{"id": "PMID:468438", "title": "Rethinking suicide: notes toward a critical epidemiology.", "content": "Where the genesis of \"disease\" owes much to causes that are social and economic in nature, epidemiology holds unrealized potential as a tool of social criticism. A particularly interesting example is provided by suicide and suicide research. Methodological difficulties are explored in detail, major findings reviewed, and the dominant interpretations of such findings criticized. Research has consistently pointed to the risks of marginal or minority status, unemployment, weak community supports, situational crises, and the pressures people are subjected to during periods of economic depression. It is argued that the sociostructural implications of such research have been systematically ignored, attention being devoted instead to more efficient management of the suicidal individual--this in spite of the lack of success of suicide prevention centers. Initial steps toward an alternative framework are outlined, with emphasis laid on the need to disaggregate the suicide act. It is further suggested that self-destruction is a far commoner--indeed, integral--part of our social environment than the bare rack of suicide statistics would suggest.", "contents": "Rethinking suicide: notes toward a critical epidemiology. Where the genesis of \"disease\" owes much to causes that are social and economic in nature, epidemiology holds unrealized potential as a tool of social criticism. A particularly interesting example is provided by suicide and suicide research. Methodological difficulties are explored in detail, major findings reviewed, and the dominant interpretations of such findings criticized. Research has consistently pointed to the risks of marginal or minority status, unemployment, weak community supports, situational crises, and the pressures people are subjected to during periods of economic depression. It is argued that the sociostructural implications of such research have been systematically ignored, attention being devoted instead to more efficient management of the suicidal individual--this in spite of the lack of success of suicide prevention centers. Initial steps toward an alternative framework are outlined, with emphasis laid on the need to disaggregate the suicide act. It is further suggested that self-destruction is a far commoner--indeed, integral--part of our social environment than the bare rack of suicide statistics would suggest."}
{"id": "PMID:468439", "title": "Convenience and the occurrence of births: induction of labor in the United States and Canada.", "content": "This paper, using data for the United States and Canada on number of births by day of the week, presents indirect evidence for the widespread incidence of the practice of elective induction. For both the United States and Canada, it is found that substantially fewer births occur on Saturdays, Sundays, and holidays than on weekdays. Controlling for such factors as prenatal care, race, education, legitimacy, birth weight, and time trend strongly suggests that the induction of labor is responsible for the patterns found. The paper concludes by discussing the framework within which the practice of elective induction of labor should be evaluated and justified.", "contents": "Convenience and the occurrence of births: induction of labor in the United States and Canada. This paper, using data for the United States and Canada on number of births by day of the week, presents indirect evidence for the widespread incidence of the practice of elective induction. For both the United States and Canada, it is found that substantially fewer births occur on Saturdays, Sundays, and holidays than on weekdays. Controlling for such factors as prenatal care, race, education, legitimacy, birth weight, and time trend strongly suggests that the induction of labor is responsible for the patterns found. The paper concludes by discussing the framework within which the practice of elective induction of labor should be evaluated and justified."}
{"id": "PMID:468440", "title": "Medicine and patriarchal violence: the social construction of a \"private\" event.", "content": "Our objectives are to describe the pattern of abuse associated with battering and to evaluate the contribution of the medical system and of broader social forces to its emergence. A pilot study of 481 women who used the emergency service of a large metropolitan hospital in the U.S. shows that battering includes a history of self-abuse and psychosocial problems, as well as repeated and escalating physical injury. In addition, although the number of battered women using the service is 10 times higher than medical personnel identify, the pattern of abuse that constitutes battering emerges only after its initial effects are presented and in conjunction with specific medical intervnetions and referrals. Examination of intervention and referral patterns suggests a staging process by which battering is socially constructed. At first, the physical trauma associated with abuse is medicated symptomatically. But the patient's persistence, the failure of the cure, and the incongruity between her problems and available medical explanations lead the provider to label the abused woman in ways that suggest she is personally responsible for her victimization. Although secondary problems such as depression, drug abuse, suicide attempts, or alcoholism derive as much from the intervention strategy adopted as from physical assault or psychopathology, they are treated as the primary problems at psychiatric and social service referral points where family maintenance is often the therapeutic goal. One consequence of this referral strategy is the stabilization of \"violent families\" in ways that virtually insure women will be abused in systematic and arbitrary ways. The use of patriarchal logic by medical providers ostensibly responding to physical trauma has less to do with individual \"sexism\" than with the political and economic constraints under which medicine operates as part of an \"extended patriarchy.\" Medicine's role in battering suggests that the services function to reconstitute the \"private\" world of patriarchal authority, with violence if necessary, against demands to socialize the labors of love.", "contents": "Medicine and patriarchal violence: the social construction of a \"private\" event. Our objectives are to describe the pattern of abuse associated with battering and to evaluate the contribution of the medical system and of broader social forces to its emergence. A pilot study of 481 women who used the emergency service of a large metropolitan hospital in the U.S. shows that battering includes a history of self-abuse and psychosocial problems, as well as repeated and escalating physical injury. In addition, although the number of battered women using the service is 10 times higher than medical personnel identify, the pattern of abuse that constitutes battering emerges only after its initial effects are presented and in conjunction with specific medical intervnetions and referrals. Examination of intervention and referral patterns suggests a staging process by which battering is socially constructed. At first, the physical trauma associated with abuse is medicated symptomatically. But the patient's persistence, the failure of the cure, and the incongruity between her problems and available medical explanations lead the provider to label the abused woman in ways that suggest she is personally responsible for her victimization. Although secondary problems such as depression, drug abuse, suicide attempts, or alcoholism derive as much from the intervention strategy adopted as from physical assault or psychopathology, they are treated as the primary problems at psychiatric and social service referral points where family maintenance is often the therapeutic goal. One consequence of this referral strategy is the stabilization of \"violent families\" in ways that virtually insure women will be abused in systematic and arbitrary ways. The use of patriarchal logic by medical providers ostensibly responding to physical trauma has less to do with individual \"sexism\" than with the political and economic constraints under which medicine operates as part of an \"extended patriarchy.\" Medicine's role in battering suggests that the services function to reconstitute the \"private\" world of patriarchal authority, with violence if necessary, against demands to socialize the labors of love."}
{"id": "PMID:468441", "title": "Nutrition and national development: the case of Chile.", "content": "This study is an historical analysis of food consumption and nutrition in Chile emphasizing the influence of political and economic factors on nutritional standards. It attempts to document and explain the persistence of malnutrition as a widespread social problem in Chile even as the country achieved a relatively advanced state of economic development and boasted an unusually progressive record of social legislation. The major findings of the study were: (a) Chile's pattern of development, social reform efforts notwithstanding, consistently discriminated against low-income groups, and (b) this discrimination perpetuated low standards of nutrition and low levels of food consumption among the country's poor and undermined the effectiveness of specific measures to alleviate malnutrition.", "contents": "Nutrition and national development: the case of Chile. This study is an historical analysis of food consumption and nutrition in Chile emphasizing the influence of political and economic factors on nutritional standards. It attempts to document and explain the persistence of malnutrition as a widespread social problem in Chile even as the country achieved a relatively advanced state of economic development and boasted an unusually progressive record of social legislation. The major findings of the study were: (a) Chile's pattern of development, social reform efforts notwithstanding, consistently discriminated against low-income groups, and (b) this discrimination perpetuated low standards of nutrition and low levels of food consumption among the country's poor and undermined the effectiveness of specific measures to alleviate malnutrition."}
{"id": "PMID:468442", "title": "Place of the indigenous and the western systems of medicine in the health services of India.", "content": "The interrelationships of the indigenous (traditional and western (modern) systems of medicine are a function of the interplay of social, economic, and political forces in the community. In India, western medicine was used as a political weapon by the colonialists to strengthen the oppressing classes and to weaken the oppressed. Not only were the masses denied access to the western system of medicine, but this system contributed to the decay and degeneration of the preexisting indigenous systems. This western and privileged-class orientation of the health services has been actively perpetuated and promoted by the postcolonial leadership of India. The issue in formulating an alternative health care system for India is essentially that of rectifying the distortions which have been brought about by various forces. The basic premise for such an alternative will be to start with the people. Action in this field will lead to a more harmonious mix between the indigenous and western systems of medicine.", "contents": "Place of the indigenous and the western systems of medicine in the health services of India. The interrelationships of the indigenous (traditional and western (modern) systems of medicine are a function of the interplay of social, economic, and political forces in the community. In India, western medicine was used as a political weapon by the colonialists to strengthen the oppressing classes and to weaken the oppressed. Not only were the masses denied access to the western system of medicine, but this system contributed to the decay and degeneration of the preexisting indigenous systems. This western and privileged-class orientation of the health services has been actively perpetuated and promoted by the postcolonial leadership of India. The issue in formulating an alternative health care system for India is essentially that of rectifying the distortions which have been brought about by various forces. The basic premise for such an alternative will be to start with the people. Action in this field will lead to a more harmonious mix between the indigenous and western systems of medicine."}
{"id": "PMID:468443", "title": "Health care and workers' self-management in Yugoslavia.", "content": "The form of communism developed in Yugoslavia since the early 1950s--called workers' self-management--has been of interest to many westerners concerned with the ideas of participatory democracy and workers' self-management. Yugoslavia's economic growth and general openness toward the West have encouraged many people to visit and to investigate the health care system as well as other aspects of the society. It is generally said that self-management is responsible for the remarkable successes that Yugoslavia has achieved. This paper suggests, on the other hand, that many of the successes achieved in the health care system are attributable largely to the fact that self-management is not allowed to work as freely as it does in other sectors of the economy.", "contents": "Health care and workers' self-management in Yugoslavia. The form of communism developed in Yugoslavia since the early 1950s--called workers' self-management--has been of interest to many westerners concerned with the ideas of participatory democracy and workers' self-management. Yugoslavia's economic growth and general openness toward the West have encouraged many people to visit and to investigate the health care system as well as other aspects of the society. It is generally said that self-management is responsible for the remarkable successes that Yugoslavia has achieved. This paper suggests, on the other hand, that many of the successes achieved in the health care system are attributable largely to the fact that self-management is not allowed to work as freely as it does in other sectors of the economy."}
{"id": "PMID:468444", "title": "Effects of tetrahydrocannabinol on arterial and intraocular hypertension.", "content": "Clinical studies were conducted to determine the relationship between simultaneous changes in heart rate, blood pressure, and intraocular pressure in systemic normotensive (N=8) and hypertensive (N=8) open-angle glaucoma patients (N=16) after inhalation of tetrahydrocannabinol (THC). Insignificant changes occurred in arterial and ocular pressures after placebo pertubations. The functional responses after 2.8% THC inhalation in sitting normotensive and hypertensive patients included invariable increases in heart rate (range 20-62 beats/min greater than control) followed by substantial decreases in systolic pressure (range 12-52 mmHg less than control), diastolic pressure (range 6-28 mmHg less than control), and intraocular pressure (range 6-21 mmHg less than control). The intensity and duration (3-4 hours) of the arterial and ocular pressure responses to THC were greater in hypertensives than in normotensive patients. The salient observation after THC inhalation was that the changes in ocular pressure paralleled the changes in blood pressure in each glaucoma patient. These findings suggest that the positive chronotropic response to THC tends to maintain cardiac output which limits further decreases in blood pressure and the capillary filtration of aqueous humor decreases or the reabsorption of aqueous humor increases because of the systemic hypotensive effect attending THC inhalation.", "contents": "Effects of tetrahydrocannabinol on arterial and intraocular hypertension. Clinical studies were conducted to determine the relationship between simultaneous changes in heart rate, blood pressure, and intraocular pressure in systemic normotensive (N=8) and hypertensive (N=8) open-angle glaucoma patients (N=16) after inhalation of tetrahydrocannabinol (THC). Insignificant changes occurred in arterial and ocular pressures after placebo pertubations. The functional responses after 2.8% THC inhalation in sitting normotensive and hypertensive patients included invariable increases in heart rate (range 20-62 beats/min greater than control) followed by substantial decreases in systolic pressure (range 12-52 mmHg less than control), diastolic pressure (range 6-28 mmHg less than control), and intraocular pressure (range 6-21 mmHg less than control). The intensity and duration (3-4 hours) of the arterial and ocular pressure responses to THC were greater in hypertensives than in normotensive patients. The salient observation after THC inhalation was that the changes in ocular pressure paralleled the changes in blood pressure in each glaucoma patient. These findings suggest that the positive chronotropic response to THC tends to maintain cardiac output which limits further decreases in blood pressure and the capillary filtration of aqueous humor decreases or the reabsorption of aqueous humor increases because of the systemic hypotensive effect attending THC inhalation."}
{"id": "PMID:468445", "title": "Bioavailability study of gitoxin in a solid dosage form.", "content": "Although the cardiotonic activity of gitoxin is known for almost half a century, this digitalis glycoside has never been used in therapy, due to its apparent lack of resorption after administration by oral route. Recent studies have demonstrated that the bioavailability of gitoxin could be upraised to 100% provided it be given as a hydroalcoholic solution. The present paper deals with the development of a solid dosage form (tablets) using a physical association of gitoxin and sodium escinate.", "contents": "Bioavailability study of gitoxin in a solid dosage form. Although the cardiotonic activity of gitoxin is known for almost half a century, this digitalis glycoside has never been used in therapy, due to its apparent lack of resorption after administration by oral route. Recent studies have demonstrated that the bioavailability of gitoxin could be upraised to 100% provided it be given as a hydroalcoholic solution. The present paper deals with the development of a solid dosage form (tablets) using a physical association of gitoxin and sodium escinate."}
{"id": "PMID:468446", "title": "Chronopharmacokinetics of dipyridamole.", "content": "The experiment carried out in 6 volunteers shows a better availability of a 75 mg single oral dose of dipyridamole administered at 600 than at 2200. The availability of dipyridamole administered at 600. The dependence of kel and t0.5(elim) on the time of drug administration is less distinct.", "contents": "Chronopharmacokinetics of dipyridamole. The experiment carried out in 6 volunteers shows a better availability of a 75 mg single oral dose of dipyridamole administered at 600 than at 2200. The availability of dipyridamole administered at 600. The dependence of kel and t0.5(elim) on the time of drug administration is less distinct."}
{"id": "PMID:468447", "title": "Gentamicin in the treatment of lower genital tract infections: level of the drug in menses, cervical mucus and vaginal fluid.", "content": "The concentration of gentamicin in serum, menses, cervical mucus and vaginal fluid of 39 women under treatment with this antibiotic (80 mg every 8 hr i. m.) was tested by the agar diffusion method. The levels of the drug in the serum varied from 1.88-5.41 micrograms/ml with a peak one hour after the last dosage. In the menses the levels ranged from 2.06-5.87 micrograms/ml with a peak also one hour after the last dosage. The concentration of gentamicin in the cervical mucus and vaginal fluid varied from 0.67-5.14 micrograms/ml and 0.63-6.43 micrograms/ml with a peak three hours after the last administration of the drug. Results are briefly discussed.", "contents": "Gentamicin in the treatment of lower genital tract infections: level of the drug in menses, cervical mucus and vaginal fluid. The concentration of gentamicin in serum, menses, cervical mucus and vaginal fluid of 39 women under treatment with this antibiotic (80 mg every 8 hr i. m.) was tested by the agar diffusion method. The levels of the drug in the serum varied from 1.88-5.41 micrograms/ml with a peak one hour after the last dosage. In the menses the levels ranged from 2.06-5.87 micrograms/ml with a peak also one hour after the last dosage. The concentration of gentamicin in the cervical mucus and vaginal fluid varied from 0.67-5.14 micrograms/ml and 0.63-6.43 micrograms/ml with a peak three hours after the last administration of the drug. Results are briefly discussed."}
{"id": "PMID:468448", "title": "Time course of clinical-chemical parameters under long-term lithium treatment.", "content": "In 40 manic-depressive patients under lithium prophylaxis the time course of the following variables has been studied over a period of 3 years (retrospective investigation): Diff. blood smear, hemoglobine, leucocytes, electrolytes in serum, electrophoresis, GPT, GOT, alcaline phosphatase, creatinine and urea in serum. Appr. 1000 blood samples were evaluated. The issue of additional psychotropic medication has been given special consideration.", "contents": "Time course of clinical-chemical parameters under long-term lithium treatment. In 40 manic-depressive patients under lithium prophylaxis the time course of the following variables has been studied over a period of 3 years (retrospective investigation): Diff. blood smear, hemoglobine, leucocytes, electrolytes in serum, electrophoresis, GPT, GOT, alcaline phosphatase, creatinine and urea in serum. Appr. 1000 blood samples were evaluated. The issue of additional psychotropic medication has been given special consideration."}
{"id": "PMID:468449", "title": "Kinetics of a rifampicin-trimethoprim combination.", "content": "Two different regimens of a rifampicin-trimethoprim combination were studied in two groups of healthy volunteers. Each regimen was administered for seven consecutive days. In the first, the treatment consisted of 300 mg of rifampicin and 80 mg of trimethoprim administered t.i.d. In the second 450 mg of rifampicin and 120 mg of trimethoprim were administered b.i.d. Data on serum levels and urinary excretion are discussed.", "contents": "Kinetics of a rifampicin-trimethoprim combination. Two different regimens of a rifampicin-trimethoprim combination were studied in two groups of healthy volunteers. Each regimen was administered for seven consecutive days. In the first, the treatment consisted of 300 mg of rifampicin and 80 mg of trimethoprim administered t.i.d. In the second 450 mg of rifampicin and 120 mg of trimethoprim were administered b.i.d. Data on serum levels and urinary excretion are discussed."}
{"id": "PMID:468450", "title": "The effect of the composition of food on the absorption of sulfameter.", "content": "The present investigation is concerned with the influence of the composition of food on the absorption of sulfameter. Six physically healthy patients each were given 2 g of sulfameter simultaneously with a high lipid, high protein and high carbohydrate test meal. This experiment was designed as a threefold crossover study, and there was a randomized assignment of patients to the different conditions. The results show that sulfameter is significantly better absorbed when administered with a high lipid meal than when given with a high protein or high carbohydrate meal, demonstrated by the areas under the serum concentration curves (AUC), by the peak serum concentration and by the cumulative renal excretion.", "contents": "The effect of the composition of food on the absorption of sulfameter. The present investigation is concerned with the influence of the composition of food on the absorption of sulfameter. Six physically healthy patients each were given 2 g of sulfameter simultaneously with a high lipid, high protein and high carbohydrate test meal. This experiment was designed as a threefold crossover study, and there was a randomized assignment of patients to the different conditions. The results show that sulfameter is significantly better absorbed when administered with a high lipid meal than when given with a high protein or high carbohydrate meal, demonstrated by the areas under the serum concentration curves (AUC), by the peak serum concentration and by the cumulative renal excretion."}
{"id": "PMID:468451", "title": "Pharmacokinetics of furagin, a new nitrofurantoin congener, on human volunteers.", "content": "The human pharmacokinetics of a nitrofurantoin congener furagin was studied after a single oral dose of 200 mg and during a 9-day continuous treatment with a dose of 100 mg t.i.d. The same dose of nitrofurantoin served as a reference medication. In the acute cross-over phase food greatly speeded up and atropine somewhat retarded the absorption of furagin, but the total absorption remained virtually unchanged as judged from the unchanged AUC values. The furagin concentrations in serum remain several hours above the MIC concentrations of many pathogenic bacteria. Despite the high concentrations in serum, the urine levels of furagin were generally lower than those of nitrofurantoin. The 24 hr recoveries in urine were 8--13% for furagin and about 36% for nitrofurantoin. In the prolonged trial furagin was absorbed and excreted in the same way as in the acute trial. On the 9th day the concentrations in serum and urine were higher than on the first day. The urinary concentrations of both furagin and nitrofurantoin always remained well above the MIC values of the most susceptible bacteria. Several volunteers complained of nightly cramps in their calves after taking furagin for some days, otherwise the side effects were minimal.", "contents": "Pharmacokinetics of furagin, a new nitrofurantoin congener, on human volunteers. The human pharmacokinetics of a nitrofurantoin congener furagin was studied after a single oral dose of 200 mg and during a 9-day continuous treatment with a dose of 100 mg t.i.d. The same dose of nitrofurantoin served as a reference medication. In the acute cross-over phase food greatly speeded up and atropine somewhat retarded the absorption of furagin, but the total absorption remained virtually unchanged as judged from the unchanged AUC values. The furagin concentrations in serum remain several hours above the MIC concentrations of many pathogenic bacteria. Despite the high concentrations in serum, the urine levels of furagin were generally lower than those of nitrofurantoin. The 24 hr recoveries in urine were 8--13% for furagin and about 36% for nitrofurantoin. In the prolonged trial furagin was absorbed and excreted in the same way as in the acute trial. On the 9th day the concentrations in serum and urine were higher than on the first day. The urinary concentrations of both furagin and nitrofurantoin always remained well above the MIC values of the most susceptible bacteria. Several volunteers complained of nightly cramps in their calves after taking furagin for some days, otherwise the side effects were minimal."}
{"id": "PMID:468452", "title": "Liver size in evaluating drug metabolizing capacity in man.", "content": "Liver size, cytochrome P-450 (P-450) concentration in liver biopsy specimens and antipyrine kinetics were studied in 112 consecutive patients undergoing diagnostic liver biopsy. Compared to subjects with normal parenchyma, those with slight or severe parenchymal alterations had enlarged livers with low P-450 concentration and slow antipyrine elimination. In the normal group, the mean P-450 concentration (+/- 1 SD) was 11.10 +/- 2.14 nmol/g liver tissue and the total amount (estimated liver weight g X P-450 concentration nmol/g) 16.06 +/- 3.29 mumol. Previous therapy with enzyme inducing drugs was associated with enlarged liver in subjects with normal histological findings and with fast antipyrine elimination and high P-450 in all groups. Antipyrine elimination rate correlated with liver weight only in subjects with normal parenchyma. The total amount of P-450 was generally more closely related to its concentration than to the estimated liver weight, although in many individual cases a large liver was able to compensate a low P-450 concentration so that the total P-450 was at the normal level. Despite normal or high total P-450, in vivo drug metabolism was impaired in subjects with altered parenchyma, suggesting that other factors were limiting antipyrine elimination in liver disease.", "contents": "Liver size in evaluating drug metabolizing capacity in man. Liver size, cytochrome P-450 (P-450) concentration in liver biopsy specimens and antipyrine kinetics were studied in 112 consecutive patients undergoing diagnostic liver biopsy. Compared to subjects with normal parenchyma, those with slight or severe parenchymal alterations had enlarged livers with low P-450 concentration and slow antipyrine elimination. In the normal group, the mean P-450 concentration (+/- 1 SD) was 11.10 +/- 2.14 nmol/g liver tissue and the total amount (estimated liver weight g X P-450 concentration nmol/g) 16.06 +/- 3.29 mumol. Previous therapy with enzyme inducing drugs was associated with enlarged liver in subjects with normal histological findings and with fast antipyrine elimination and high P-450 in all groups. Antipyrine elimination rate correlated with liver weight only in subjects with normal parenchyma. The total amount of P-450 was generally more closely related to its concentration than to the estimated liver weight, although in many individual cases a large liver was able to compensate a low P-450 concentration so that the total P-450 was at the normal level. Despite normal or high total P-450, in vivo drug metabolism was impaired in subjects with altered parenchyma, suggesting that other factors were limiting antipyrine elimination in liver disease."}
{"id": "PMID:468460", "title": "Topical mechlorethamine for psoriasis. An attempt to avoid the development of sensitization by the use of a topical tolerogenic schedule.", "content": "The topical application of mechlorethamine is associated with a high incidence of irritant contact dermatitis. An attempt was made to avoid this complication by the use of a topical tolerogenic schedule. Seven of ten patients developed contact dermatitis to the medication after they had completed the schedule, The use of this medication in its present form is unlikely to be practical value in the treatment of psoriasis.", "contents": "Topical mechlorethamine for psoriasis. An attempt to avoid the development of sensitization by the use of a topical tolerogenic schedule. The topical application of mechlorethamine is associated with a high incidence of irritant contact dermatitis. An attempt was made to avoid this complication by the use of a topical tolerogenic schedule. Seven of ten patients developed contact dermatitis to the medication after they had completed the schedule, The use of this medication in its present form is unlikely to be practical value in the treatment of psoriasis."}
{"id": "PMID:468461", "title": "Basal cell nevus syndrome and adenocarcinoma of the colon. Response to systemic fluorouracil.", "content": "A patient with basal nervus syndrome was found to have an adenocarcinoma of the colon. This association, probably fortuitous, made it possible to study the effect of intravenous 5-fluorouracil on the cutaneous basal cell tumors. There was no reaction to this treatment. Subsequently, all visible basal cell tumors were satisfactorily eradicated with topical 5-fluorouracil.", "contents": "Basal cell nevus syndrome and adenocarcinoma of the colon. Response to systemic fluorouracil. A patient with basal nervus syndrome was found to have an adenocarcinoma of the colon. This association, probably fortuitous, made it possible to study the effect of intravenous 5-fluorouracil on the cutaneous basal cell tumors. There was no reaction to this treatment. Subsequently, all visible basal cell tumors were satisfactorily eradicated with topical 5-fluorouracil."}
{"id": "PMID:468462", "title": "A fractional charge model for empirical calculations of peptide-water interactions.", "content": "The properties of an empirical model of interaction between a water molecule and polar groups of peptides or small peptides are explored. The H2O molecule is represented by a four-point charges distribution. In electron donor groups, a point charge is located on the axis of the lone pairs orbitals in order to introduce some directionality in hydrogen bonds. The effective potential is approximated by the sum of the coulombic interactions between point charges distribution and of a 6--12 atom-atom potential. The coefficients of this last potential are first adjusted by simulating the geometry of the water dimer. Equilibrium configurations of associated polar molecules and H2O predicted by the model are found to be in good agreement with those resulting from more sophisticated ab initio SCF calculations. Interactions between H2O and the side-chains of the cyclic dipeptide C(L-Thr-L-His) are then calculated. It is shown that internal bridging by water is an essential effect of the solvent. The experimental position of the H2O molecule is reproduced, stability of which depends also on intermolecular interactions.", "contents": "A fractional charge model for empirical calculations of peptide-water interactions. The properties of an empirical model of interaction between a water molecule and polar groups of peptides or small peptides are explored. The H2O molecule is represented by a four-point charges distribution. In electron donor groups, a point charge is located on the axis of the lone pairs orbitals in order to introduce some directionality in hydrogen bonds. The effective potential is approximated by the sum of the coulombic interactions between point charges distribution and of a 6--12 atom-atom potential. The coefficients of this last potential are first adjusted by simulating the geometry of the water dimer. Equilibrium configurations of associated polar molecules and H2O predicted by the model are found to be in good agreement with those resulting from more sophisticated ab initio SCF calculations. Interactions between H2O and the side-chains of the cyclic dipeptide C(L-Thr-L-His) are then calculated. It is shown that internal bridging by water is an essential effect of the solvent. The experimental position of the H2O molecule is reproduced, stability of which depends also on intermolecular interactions."}
{"id": "PMID:468463", "title": "Studies on protein folding, unfolding and fluctuations by computer simulation. IV. Hydrophobic interactions.", "content": "The theoretical model of proteins on the two-dimensional square lattice, introduced previously, is extended to include the hydrophobic interactions. Two proteins, whose native conformations have different folded patterns, are studied. Units in the protein chains are classified into polar units and nonpolar units. If there is a vacant lattice point next to a nonpolar unit, it is interpreted as being occupied by solvent water and the entropy of the system is assumed to decrease by a certain amount. Besides these hydrophobic free energies, the specific long-range interactions studied in previous papers are assumed to be operative in a protein chain. Equilibrium properties of the folding and unfolding transitions of the two proteins are found to be similar, even though one of them was predicted, based on the one globule model of the transitions, to unfold through a significant intermediate state (or at least to show a tendency toward such a behavior), when the hydrophobic interactions are strongly weighted. The failure of this prediction led to the development of a more refined model of transitions; a non-interacting local structure model. The hydrophobic interactions assumed here have a character of non-specific long-range interactions. Because of this character the hydrophobic interactions have the effect of decelerating the folding kinetics. The deceleration effect is less pronounced in one of the two proteins, whose native conformation is stabilized by many pairs of medium-range interactions. It is therefore inferred that the medium-range interactions have the power to cope with the decelerating effect of the non-specific hydrophobic interactions.", "contents": "Studies on protein folding, unfolding and fluctuations by computer simulation. IV. Hydrophobic interactions. The theoretical model of proteins on the two-dimensional square lattice, introduced previously, is extended to include the hydrophobic interactions. Two proteins, whose native conformations have different folded patterns, are studied. Units in the protein chains are classified into polar units and nonpolar units. If there is a vacant lattice point next to a nonpolar unit, it is interpreted as being occupied by solvent water and the entropy of the system is assumed to decrease by a certain amount. Besides these hydrophobic free energies, the specific long-range interactions studied in previous papers are assumed to be operative in a protein chain. Equilibrium properties of the folding and unfolding transitions of the two proteins are found to be similar, even though one of them was predicted, based on the one globule model of the transitions, to unfold through a significant intermediate state (or at least to show a tendency toward such a behavior), when the hydrophobic interactions are strongly weighted. The failure of this prediction led to the development of a more refined model of transitions; a non-interacting local structure model. The hydrophobic interactions assumed here have a character of non-specific long-range interactions. Because of this character the hydrophobic interactions have the effect of decelerating the folding kinetics. The deceleration effect is less pronounced in one of the two proteins, whose native conformation is stabilized by many pairs of medium-range interactions. It is therefore inferred that the medium-range interactions have the power to cope with the decelerating effect of the non-specific hydrophobic interactions."}
{"id": "PMID:468464", "title": "Syntheses and characterization of tetra-gamma-benzyl-L-glutamates.", "content": "Monodisperse tetra-gamma-benzyl-L-glutamates are prepared by coupling two kinds of dimers by means of dicyclohexylcarbodiimide and then converting a terminal group into the other. An o-nitrophenylthio group is used for blocking the amino terminal, and a p-nitrophenyl ester or an ethylamide substitutes the carboxyl end. The amino-terminal-deblocked tetramer, HCl,H-[Glu(OBzl)] 4-NHEt, is alos prepared. Infrared spectra in nujol mull indicate that all the tetramers are in the beta-structure of antiparallel chains in the solid state. Circular dichroism spectra show that the amino-terminal deblocked tetramer keeps the beta-conformation in ethylene dichloride, while it has the solvated sigma-conformation in trifluoroethanol and in dioxane.", "contents": "Syntheses and characterization of tetra-gamma-benzyl-L-glutamates. Monodisperse tetra-gamma-benzyl-L-glutamates are prepared by coupling two kinds of dimers by means of dicyclohexylcarbodiimide and then converting a terminal group into the other. An o-nitrophenylthio group is used for blocking the amino terminal, and a p-nitrophenyl ester or an ethylamide substitutes the carboxyl end. The amino-terminal-deblocked tetramer, HCl,H-[Glu(OBzl)] 4-NHEt, is alos prepared. Infrared spectra in nujol mull indicate that all the tetramers are in the beta-structure of antiparallel chains in the solid state. Circular dichroism spectra show that the amino-terminal deblocked tetramer keeps the beta-conformation in ethylene dichloride, while it has the solvated sigma-conformation in trifluoroethanol and in dioxane."}
{"id": "PMID:468466", "title": "Quantitation of some amino-terminal residues in proteins using 3H-labelled dansyl chloride and 14C-labelled amino acids.", "content": "A method for quantitation of amino-terminal residues in proteins is presented. The method is a modification of a double isotope-labelling technique, using 3H-labelled dansyl chloride and 14C-labelled amino acids as internal standards. The method is demonstrated on human fibrinogen, horse myoglobin and on mouse myoloma IgA. A linear relationship between the ratio 3H/14C in the separated amino-terminal amino acid of the protein and the amount of protein added in the labelling mixture was obtained with standard deviations of +/- 7.4% +/- 3.4% and +/- 10.3%, respectively. An application of the method is demonstrated by measuring the increase in amino-terminal glycine in fibrinogen following the proteolytic action of thrombin. The method seems to be useful when 0.1 nmol or more of protein is used.", "contents": "Quantitation of some amino-terminal residues in proteins using 3H-labelled dansyl chloride and 14C-labelled amino acids. A method for quantitation of amino-terminal residues in proteins is presented. The method is a modification of a double isotope-labelling technique, using 3H-labelled dansyl chloride and 14C-labelled amino acids as internal standards. The method is demonstrated on human fibrinogen, horse myoglobin and on mouse myoloma IgA. A linear relationship between the ratio 3H/14C in the separated amino-terminal amino acid of the protein and the amount of protein added in the labelling mixture was obtained with standard deviations of +/- 7.4% +/- 3.4% and +/- 10.3%, respectively. An application of the method is demonstrated by measuring the increase in amino-terminal glycine in fibrinogen following the proteolytic action of thrombin. The method seems to be useful when 0.1 nmol or more of protein is used."}
{"id": "PMID:468467", "title": "The avoidance of sucrose by thiamine-deficient rats.", "content": "Rats were fed carbohydrate-free diets without thiamine. Sucrose was offered separately, either before the thiamine was removed, or when it was removed, or at varying periods after it was removed. In the absence of dietary thiamine, sucrose consumption began at a high level, but was then reduced. Thereafter, death due to thiamine deficiency followed at intervals that varied from 4 weeks to 12 months. Survival was shortest in those rats that ate sucrose in largest amounts or at shortest intervals; survival was longest in those rats that avoided sucrose altogether for long periods. Sex or age did not appear to affect survival. The results are interpreted as demonstrating a conflict between the avoidance of sucrose so as to avoid the unpleasant symptoms of thiamine deficiency, and the consumption of sucrose so as to obtain the pleasure of its high palatability.", "contents": "The avoidance of sucrose by thiamine-deficient rats. Rats were fed carbohydrate-free diets without thiamine. Sucrose was offered separately, either before the thiamine was removed, or when it was removed, or at varying periods after it was removed. In the absence of dietary thiamine, sucrose consumption began at a high level, but was then reduced. Thereafter, death due to thiamine deficiency followed at intervals that varied from 4 weeks to 12 months. Survival was shortest in those rats that ate sucrose in largest amounts or at shortest intervals; survival was longest in those rats that avoided sucrose altogether for long periods. Sex or age did not appear to affect survival. The results are interpreted as demonstrating a conflict between the avoidance of sucrose so as to avoid the unpleasant symptoms of thiamine deficiency, and the consumption of sucrose so as to obtain the pleasure of its high palatability."}
{"id": "PMID:468468", "title": "The effects of riboflavin administration on iron metabolism parameters in a school-going population.", "content": "The effect of riboflavin administration on iron metabolism was studied in a group of riboflavin-deficient children with Erythrocyte Glutathione Reductase (EC 1.6.4.2) reactivation above 1.20. The results have shown that the administration of riboflavin has resulted in a decrease of serum iron as well as in transferrin saturation which was accompanied by an increase in blood hemoglobin in subjects with initially lower hemoglobin values (less than or equal to 13.5 g/100 ml). There was, however, no change in blood hemoglobin in the group with initially higher hemoglobin values (greater than or equal to 14.0 g/100 ml). The results suggest that inadequate riboflavin intake may affect the iron utilization. The possible mechanism of riboflavin action on iron utilization is discussed.", "contents": "The effects of riboflavin administration on iron metabolism parameters in a school-going population. The effect of riboflavin administration on iron metabolism was studied in a group of riboflavin-deficient children with Erythrocyte Glutathione Reductase (EC 1.6.4.2) reactivation above 1.20. The results have shown that the administration of riboflavin has resulted in a decrease of serum iron as well as in transferrin saturation which was accompanied by an increase in blood hemoglobin in subjects with initially lower hemoglobin values (less than or equal to 13.5 g/100 ml). There was, however, no change in blood hemoglobin in the group with initially higher hemoglobin values (greater than or equal to 14.0 g/100 ml). The results suggest that inadequate riboflavin intake may affect the iron utilization. The possible mechanism of riboflavin action on iron utilization is discussed."}
{"id": "PMID:468469", "title": "Sectrofluorimetric micromethod for determining riboflavin in the blood of new-born babies and their mothers.", "content": "A microdetermination of riboflavin on the blood of new-born babies and their mothers was developed on the basis of hydrolysis of the blood in a trichloroacetic acid medium, separation of riboflavin and FMN on a Florisil column and measurement of the content spectrofluorimetrically by the standard addition method after elution with collidine buffer. The sensitivity of the method is 0.01 microgram/ml, the blood sample size 0.5--1.0 ml. The normal level in new-born babies in the first day of life was found to have an average value of 17.1+/-2.4 microgram/100 ml, in women 14.2+/-2.5 microgram/100 ml; the contents in cord blood and maternal vein blood were also determined.", "contents": "Sectrofluorimetric micromethod for determining riboflavin in the blood of new-born babies and their mothers. A microdetermination of riboflavin on the blood of new-born babies and their mothers was developed on the basis of hydrolysis of the blood in a trichloroacetic acid medium, separation of riboflavin and FMN on a Florisil column and measurement of the content spectrofluorimetrically by the standard addition method after elution with collidine buffer. The sensitivity of the method is 0.01 microgram/ml, the blood sample size 0.5--1.0 ml. The normal level in new-born babies in the first day of life was found to have an average value of 17.1+/-2.4 microgram/100 ml, in women 14.2+/-2.5 microgram/100 ml; the contents in cord blood and maternal vein blood were also determined."}
{"id": "PMID:468470", "title": "Vitamin C deficiency in guinea pigs: changes in urinary excretion of proline, hydroxyproline and total amino nitrogen.", "content": "1. The age-related decrease in hydroxyproline : creatinine ratio in young guinea pigs was significantly smaller in vitamin C-deficient animals than in pair-fed controls. The same was true for proline : creatinine and total amino nitrogen : creatinine ratios, but hydroxyproline : total amino nitrogen and proline : total amino nitrogen ratios were not significantly affected by deficiency. 2. Although the proline : hydroxyproline ratio was unaffected in unfractionated urine, acute or chronic deficiency produced a small but significant increase in this ratio in collagenase digests of the acetone-insoluble fraction. 3. In scorbutic animals, therefore, collagen probably turns over more rapidly than in animals matched for inanition. Some at least, of this increase could represent the rapid turnover of underhydroxylated nascent collagen. Because it contains the degradation products from collagen from many tissues, differing widely in sensitivity to vitamin C status, the urine is unlikely, however, to provide a specific and sensitive functional index of vitamin C status.", "contents": "Vitamin C deficiency in guinea pigs: changes in urinary excretion of proline, hydroxyproline and total amino nitrogen. 1. The age-related decrease in hydroxyproline : creatinine ratio in young guinea pigs was significantly smaller in vitamin C-deficient animals than in pair-fed controls. The same was true for proline : creatinine and total amino nitrogen : creatinine ratios, but hydroxyproline : total amino nitrogen and proline : total amino nitrogen ratios were not significantly affected by deficiency. 2. Although the proline : hydroxyproline ratio was unaffected in unfractionated urine, acute or chronic deficiency produced a small but significant increase in this ratio in collagenase digests of the acetone-insoluble fraction. 3. In scorbutic animals, therefore, collagen probably turns over more rapidly than in animals matched for inanition. Some at least, of this increase could represent the rapid turnover of underhydroxylated nascent collagen. Because it contains the degradation products from collagen from many tissues, differing widely in sensitivity to vitamin C status, the urine is unlikely, however, to provide a specific and sensitive functional index of vitamin C status."}
{"id": "PMID:468471", "title": "Effect of retinol on the molecular species of hepatic phosphatidyl choline in young rats.", "content": "Effect of feeding 1.5 and 12 mg retinol respectively for two days to young rats on the distribution and synthesis of molecular species of PC in liver was studied. A significant decrease was observed in the amounts of trienoic and tetraeonic PC in rats fed 12 mg retinol. Feeding of retinol did not affect the synthesis of PC via CDP - choline pathway but enhanced the synthesis of hexaenoic PC via N-methylation of PE.", "contents": "Effect of retinol on the molecular species of hepatic phosphatidyl choline in young rats. Effect of feeding 1.5 and 12 mg retinol respectively for two days to young rats on the distribution and synthesis of molecular species of PC in liver was studied. A significant decrease was observed in the amounts of trienoic and tetraeonic PC in rats fed 12 mg retinol. Feeding of retinol did not affect the synthesis of PC via CDP - choline pathway but enhanced the synthesis of hexaenoic PC via N-methylation of PE."}
{"id": "PMID:468472", "title": "The nutritional value of irradiated casein-fat mixtures-effect of anaerobic conditions, storage time, added vitamin E and degree of unsaturation of lipids.", "content": "The effect of oxygen elimination during irradiation (5 Mrad) and subsequent storage, storage time (12 weeks), degree of lipid-unsaturation and added antioxidant (vitamin E) in protein-lipid mixtures (casein : fat - 11.1 : 4) on the nutritive value of protein was studied during 8-day nitrogen balance trials in 12 groups of 6 weanling rats each. When casein-cocofat-cornoil mixtures were irradiated and stored under aerobic conditions a reduction of NPU appeared upon prolonged storage time, which was prevented by excluding oxygen during irradiation and storage. When the lipid component consisted entirely of cocofat no loss in NPU occurred under aerobic processing and storage conditions throughout the 12 week storage period. Replacement of cocofat through sunfloweroil resulted in a drastic reduction of NPU and in growth inhibition, which was not prevented by the addition of vitamin E (0.05 g/100 g lipid). Irradiation of casein-lipid mixtures resulted in a significant increase of carbonyl compounds. A further increase was effectively prevented by elimination of oxygen during irradiation and storage. A drastic increase of carbonyl compounds as well as a significant reduction of available lysine occurred in casein-sunfloweroil mixtures. Added vitamin E afforded only limited protection against these changes.", "contents": "The nutritional value of irradiated casein-fat mixtures-effect of anaerobic conditions, storage time, added vitamin E and degree of unsaturation of lipids. The effect of oxygen elimination during irradiation (5 Mrad) and subsequent storage, storage time (12 weeks), degree of lipid-unsaturation and added antioxidant (vitamin E) in protein-lipid mixtures (casein : fat - 11.1 : 4) on the nutritive value of protein was studied during 8-day nitrogen balance trials in 12 groups of 6 weanling rats each. When casein-cocofat-cornoil mixtures were irradiated and stored under aerobic conditions a reduction of NPU appeared upon prolonged storage time, which was prevented by excluding oxygen during irradiation and storage. When the lipid component consisted entirely of cocofat no loss in NPU occurred under aerobic processing and storage conditions throughout the 12 week storage period. Replacement of cocofat through sunfloweroil resulted in a drastic reduction of NPU and in growth inhibition, which was not prevented by the addition of vitamin E (0.05 g/100 g lipid). Irradiation of casein-lipid mixtures resulted in a significant increase of carbonyl compounds. A further increase was effectively prevented by elimination of oxygen during irradiation and storage. A drastic increase of carbonyl compounds as well as a significant reduction of available lysine occurred in casein-sunfloweroil mixtures. Added vitamin E afforded only limited protection against these changes."}
{"id": "PMID:468473", "title": "Effect of dietary selenium and vitamin E on the antioxiant defense systems of rat erythrocytes.", "content": "The effect of dietary selenium and vitamin E on the important cellular antioxidant defense systems was studied in rat erythrocytes. Weanling male Sprague-Dawley rats were fed a basal selenium and vitamin E deficient diet and supplemented with either none or 0.5 ppm selenium and either none or 45 ppm vitamin E for 35 or 40 days. Depletion of dietary selenium resulted in marked decrease of glutathione (GSH) peroxidase in the red cells, but the levels of GSH, catalase and superoxide dismutase were not significantly altered. The red cells of rats fed the basal diet deficient in both selenium and vitamin E had significantly lower levels of GSH and GSH peroxidase, but not of catalase and superoxide dismutase, than in those fed the basal diet and supplemented with either selenium, vitamin E or both. The results suggest that depletion of dietary selenium and vitamin may have a precipitate effect on lowering the levels of GSH and GSH peroxidase in rat erytyrocytes.", "contents": "Effect of dietary selenium and vitamin E on the antioxiant defense systems of rat erythrocytes. The effect of dietary selenium and vitamin E on the important cellular antioxidant defense systems was studied in rat erythrocytes. Weanling male Sprague-Dawley rats were fed a basal selenium and vitamin E deficient diet and supplemented with either none or 0.5 ppm selenium and either none or 45 ppm vitamin E for 35 or 40 days. Depletion of dietary selenium resulted in marked decrease of glutathione (GSH) peroxidase in the red cells, but the levels of GSH, catalase and superoxide dismutase were not significantly altered. The red cells of rats fed the basal diet deficient in both selenium and vitamin E had significantly lower levels of GSH and GSH peroxidase, but not of catalase and superoxide dismutase, than in those fed the basal diet and supplemented with either selenium, vitamin E or both. The results suggest that depletion of dietary selenium and vitamin may have a precipitate effect on lowering the levels of GSH and GSH peroxidase in rat erytyrocytes."}
{"id": "PMID:468474", "title": "The ubiquitousity of labelled tocopherol in the cockerel body.", "content": "A recovery experiment with a young cock weighing 1 kg showed that after 3 days, radioactivity from the labelled vitamin E was distributed to tissues of all of the 11 organs being investigated. The liver had the highest concentration of the label, but gut, fat tissue and muscle had a total content each comparable with that of the liver. The air expired during the 3 days also exposed radioactivity.", "contents": "The ubiquitousity of labelled tocopherol in the cockerel body. A recovery experiment with a young cock weighing 1 kg showed that after 3 days, radioactivity from the labelled vitamin E was distributed to tissues of all of the 11 organs being investigated. The liver had the highest concentration of the label, but gut, fat tissue and muscle had a total content each comparable with that of the liver. The air expired during the 3 days also exposed radioactivity."}
{"id": "PMID:468475", "title": "Food intake patterns of infants with high serum cholesterol level at six months.", "content": "In our longitudinal study on the influence of nutrition upon health during infancy we found in a cohort of 556 infants in good health, 6.4% of infants at six months with a total serum cholesterol level above 200 mg/100 ml. Because of the known importance of hypercholesterolemia for health we studied the role of the food intake patterns during the first six months in relation to the serum cholesterol. We analysed the 24 hours dietary recalls, the dietary habits and a set of biochemical determinations used for evaluation of nutritional status: 35 infants, 21 girls and 14 boys, were compared with a control group matched for sex and birth date. There were no differences in mean body weight and socio-demographic characteristics. Daily energy and protein intake was higher in the study group. Analysing milk consumption we found that the daily intake was always higher in the high cholesterol group. The solid foods were introduced earlier in the study group, i.e. for 46% of infants in the second week of life. The serum levels of vitamins A, C, and E, and of triglyceride were higher in the high cholesterol groups. At twelve months 33% of infants with the cholesterol level above 200 mg/100 ml we included in the studied group remain still with a high level. This tendency underlines the need of further research.", "contents": "Food intake patterns of infants with high serum cholesterol level at six months. In our longitudinal study on the influence of nutrition upon health during infancy we found in a cohort of 556 infants in good health, 6.4% of infants at six months with a total serum cholesterol level above 200 mg/100 ml. Because of the known importance of hypercholesterolemia for health we studied the role of the food intake patterns during the first six months in relation to the serum cholesterol. We analysed the 24 hours dietary recalls, the dietary habits and a set of biochemical determinations used for evaluation of nutritional status: 35 infants, 21 girls and 14 boys, were compared with a control group matched for sex and birth date. There were no differences in mean body weight and socio-demographic characteristics. Daily energy and protein intake was higher in the study group. Analysing milk consumption we found that the daily intake was always higher in the high cholesterol group. The solid foods were introduced earlier in the study group, i.e. for 46% of infants in the second week of life. The serum levels of vitamins A, C, and E, and of triglyceride were higher in the high cholesterol groups. At twelve months 33% of infants with the cholesterol level above 200 mg/100 ml we included in the studied group remain still with a high level. This tendency underlines the need of further research."}
{"id": "PMID:468476", "title": "The effects of antioxidants on the content of polyunsaturated fatty acids in the hen's egg.", "content": "In experiments to see whether, in the possible interests of human health, the polyunsaturated fatty acid (PUFA) content of the chicken's egg can be increased by nutritional means, three strains of hen, light, medium, and heavy, each at the peak of lay, were first fed a basal, commercial, low-fat diet. The hens were then transferred to one of the following diets: basal + safflower oil (SO); basal + SO + butylated hydroxytoluene; or basal + SO + dl-a-toco-pheryl acetate. The diets were designated \"Blank\", \"BHT\", and \"Vitamin E\", respectively, the second and third containing the added antioxidants. The eggs produced were weighed, and their yolks weighed and analysed for lipid components. Additional of SO (7.5%) to the basal diet led to the PUFA content of the yolk lipids rising by 15.4% (linoleic acid, 14.1%), the magnitude of the increases being unaffected by the antioxidants. Diet \"BHT\" produced larger eggs and yolks than the other diets, but the proportion of yolk was the same on the three types of feed. The total cholesterol content of egg yolks was significantly affected neither by diet, nor by strain or age of hen. The implications of these results are discussed.", "contents": "The effects of antioxidants on the content of polyunsaturated fatty acids in the hen's egg. In experiments to see whether, in the possible interests of human health, the polyunsaturated fatty acid (PUFA) content of the chicken's egg can be increased by nutritional means, three strains of hen, light, medium, and heavy, each at the peak of lay, were first fed a basal, commercial, low-fat diet. The hens were then transferred to one of the following diets: basal + safflower oil (SO); basal + SO + butylated hydroxytoluene; or basal + SO + dl-a-toco-pheryl acetate. The diets were designated \"Blank\", \"BHT\", and \"Vitamin E\", respectively, the second and third containing the added antioxidants. The eggs produced were weighed, and their yolks weighed and analysed for lipid components. Additional of SO (7.5%) to the basal diet led to the PUFA content of the yolk lipids rising by 15.4% (linoleic acid, 14.1%), the magnitude of the increases being unaffected by the antioxidants. Diet \"BHT\" produced larger eggs and yolks than the other diets, but the proportion of yolk was the same on the three types of feed. The total cholesterol content of egg yolks was significantly affected neither by diet, nor by strain or age of hen. The implications of these results are discussed."}
{"id": "PMID:468477", "title": "Iron intake by teenage girls and by pregnant women.", "content": "A dietary study among 110 teenage girls and 87 pregnant women revealed that their mean iron intake was lower than the Recommended Dietary Allowances. The explanation is assumed to be a relatively low energy intake. Considering the composition of Scandinavian food, in which about 40% of the energy calories are derived from animal products, it is suggested that about 90% of all pregnant women can cover their iron requirement without further supplements.", "contents": "Iron intake by teenage girls and by pregnant women. A dietary study among 110 teenage girls and 87 pregnant women revealed that their mean iron intake was lower than the Recommended Dietary Allowances. The explanation is assumed to be a relatively low energy intake. Considering the composition of Scandinavian food, in which about 40% of the energy calories are derived from animal products, it is suggested that about 90% of all pregnant women can cover their iron requirement without further supplements."}
{"id": "PMID:468478", "title": "Ratio of hemoglobin iron to liver in rats fed different amounts of iron.", "content": "Groups of young rats were fed diets containing amounts of iron designed to provide 0.1, 0.3, 1, 3, 10 or 30-times the iron requirement for the rat, and containing two different levels of protein. Iron deficiency anemia was most pronounced in rats receiving the larger amount of protein. In the case of most normal diets, the ratio of iron in the hemoglobin to iron stored in the liver was 26 +/- 6. This is in marked contrast with the ratio of 4.3 found in rats fed a common breakfast cereal.", "contents": "Ratio of hemoglobin iron to liver in rats fed different amounts of iron. Groups of young rats were fed diets containing amounts of iron designed to provide 0.1, 0.3, 1, 3, 10 or 30-times the iron requirement for the rat, and containing two different levels of protein. Iron deficiency anemia was most pronounced in rats receiving the larger amount of protein. In the case of most normal diets, the ratio of iron in the hemoglobin to iron stored in the liver was 26 +/- 6. This is in marked contrast with the ratio of 4.3 found in rats fed a common breakfast cereal."}
{"id": "PMID:468479", "title": "How fair is the culture I.Q. test?", "content": "If a measure of intelligence is culturally biased it is assumed that disadvantaged individuals will score lower on it than on a less biased instrument. The Cattell purports to be \"culture fair\" and it was compared to the WISC with a delinquent juvenile population, controlling for both order effects and individual administration. The results reveal consistently lower mean I.Q. scores for the Cattell under all conditions. The Cattell appears to be a more difficult test for this population. It is concluded that considerable caution should be exercised inusing the Cattell as a culture fair method of I.Q. evaluation with underprivileged delinquent youth.", "contents": "How fair is the culture I.Q. test? If a measure of intelligence is culturally biased it is assumed that disadvantaged individuals will score lower on it than on a less biased instrument. The Cattell purports to be \"culture fair\" and it was compared to the WISC with a delinquent juvenile population, controlling for both order effects and individual administration. The results reveal consistently lower mean I.Q. scores for the Cattell under all conditions. The Cattell appears to be a more difficult test for this population. It is concluded that considerable caution should be exercised inusing the Cattell as a culture fair method of I.Q. evaluation with underprivileged delinquent youth."}
{"id": "PMID:468481", "title": "Occupational therapy and psychiatry.", "content": "The origin of occupational therapy is deeply rooted in psychiatry, and in psychiatry occupational therapy often found the support and guidance necessary for its deveolpment and recognition. Today this close association no longer exists. Some factors leading to this professional distance have been outlined and changes conducive to a greater awareness of the role and function of occupational therapy in psychiatry have been suggested. An attempt has also been made to clarify the concept of occupational therapy and to discuss the contribution of the occupational therapist in certain specific areas of psychiatric treatment.", "contents": "Occupational therapy and psychiatry. The origin of occupational therapy is deeply rooted in psychiatry, and in psychiatry occupational therapy often found the support and guidance necessary for its deveolpment and recognition. Today this close association no longer exists. Some factors leading to this professional distance have been outlined and changes conducive to a greater awareness of the role and function of occupational therapy in psychiatry have been suggested. An attempt has also been made to clarify the concept of occupational therapy and to discuss the contribution of the occupational therapist in certain specific areas of psychiatric treatment."}
{"id": "PMID:468482", "title": "The long-distance psychiatric patient in the emergency room. Insights regarding travel and mental illness.", "content": "This paper deals with the motivational, demographic and clinical characteristics of individuals who seek psychiatric help at facilities located a great distance from their places of residence. The study identifies two subgroups within this category. The first (Intentional or Type A) group is constituted by persons who, owing to their concerns with the social stigma of mental illness, or driven by the desire for a magical solution to their problems, purposely undertake the journey to a distantly located psychiatric center. The second (Incidental or Type B) group consists of vacationers, hitch-hikers and skid-row individuals. While these \"long distance patients\" (LDP) did not show any differences when compared with the remainder of clinic attendance on demographic variables, distinct clinical features differentiated the two populations. Most significantly the LDP had a higher incidence of schizophrenia and alcohol related problems. The difference was entirely accounted for by the Type B subgroup ((LDP less than 0.01; Type A, NS; Type B less than 0.001). Implications of this finding have been discussed in view of the suggested relationships between travel and mental illness. Administrative problems of the LDP have been briefly commented upon.", "contents": "The long-distance psychiatric patient in the emergency room. Insights regarding travel and mental illness. This paper deals with the motivational, demographic and clinical characteristics of individuals who seek psychiatric help at facilities located a great distance from their places of residence. The study identifies two subgroups within this category. The first (Intentional or Type A) group is constituted by persons who, owing to their concerns with the social stigma of mental illness, or driven by the desire for a magical solution to their problems, purposely undertake the journey to a distantly located psychiatric center. The second (Incidental or Type B) group consists of vacationers, hitch-hikers and skid-row individuals. While these \"long distance patients\" (LDP) did not show any differences when compared with the remainder of clinic attendance on demographic variables, distinct clinical features differentiated the two populations. Most significantly the LDP had a higher incidence of schizophrenia and alcohol related problems. The difference was entirely accounted for by the Type B subgroup ((LDP less than 0.01; Type A, NS; Type B less than 0.001). Implications of this finding have been discussed in view of the suggested relationships between travel and mental illness. Administrative problems of the LDP have been briefly commented upon."}
{"id": "PMID:468483", "title": "The Oedipus complex or hey, Mom. Where is Daddy?", "content": "After investigating as much literature as I could on the Oedipus Complex or whatever name is interspersed, such as sex-role differentiation, identification, etc. the following conclusions were significant. Possible disturbance in sex-role identification, child develops avoidance systems and rejects parents, homosexuality, emotional maladjustment, shyness, resentfulness, dependency, harder to train, not \"manly enough\", apprehension, immaturity, compensatory masculinity, anti-social behaviour such as truancy, damaging or destroying private or public property, having premarital and extra matital sex relations and theft of property. Father's presence then, is of the utmost importance in the rearing of their children, particularly sons. They are necessary in the sharing with mothers of all phases of child.", "contents": "The Oedipus complex or hey, Mom. Where is Daddy? After investigating as much literature as I could on the Oedipus Complex or whatever name is interspersed, such as sex-role differentiation, identification, etc. the following conclusions were significant. Possible disturbance in sex-role identification, child develops avoidance systems and rejects parents, homosexuality, emotional maladjustment, shyness, resentfulness, dependency, harder to train, not \"manly enough\", apprehension, immaturity, compensatory masculinity, anti-social behaviour such as truancy, damaging or destroying private or public property, having premarital and extra matital sex relations and theft of property. Father's presence then, is of the utmost importance in the rearing of their children, particularly sons. They are necessary in the sharing with mothers of all phases of child."}
{"id": "PMID:468484", "title": "Scanning electron microscopic investigations on the morphology and phase conversions of uroliths.", "content": "The most frequent structures of the majority of possible urolith components are assessed by scanning electron microscopic investigations. Typical examples of the conversion of uric acid dihydrate into uric acid, weddellite into whewellite and MgHN4PO4 . 6 H20 into MgNH4PO4 . H2O are given.", "contents": "Scanning electron microscopic investigations on the morphology and phase conversions of uroliths. The most frequent structures of the majority of possible urolith components are assessed by scanning electron microscopic investigations. Typical examples of the conversion of uric acid dihydrate into uric acid, weddellite into whewellite and MgHN4PO4 . 6 H20 into MgNH4PO4 . H2O are given."}
{"id": "PMID:468485", "title": "Analysis of urinary tract infections in patients undergoing prostatectomy.", "content": "Analysis is presented of 260 patients on whom in the years 1974--1976 an operation for adenoma removal was performed. 115 patients (44.2%) had a permanently placed catheter or were repeatedly catheterized before operation, and 145 (55.8%) were able to urinate without any need for catheterization. In the group of preoperatively catheterized patients infection of the urinary system was demonstrated in 84 cases, which amounts to 75%. Among patients not catheterized before operation, infection already existed in 29 cases (20%). Operation by Millin's method was carried out in 181 patients; non-infected urine was postoperatively found in 40 cases, which amounts to 22.3%. 79 patients were operated on by the transvesical method; in this group non-infected urine was found in four cases, i.e. 5.1%. Calculated x2 indicated a significant connection between the investigated items, consisting in a much more frequent occurrence of urinary system infection in patients operated on by the transvesical method. The duration of catheter reposition in the urinary bladder after prostatectomy considerably influences the number of postoperative infections of the urinary system.", "contents": "Analysis of urinary tract infections in patients undergoing prostatectomy. Analysis is presented of 260 patients on whom in the years 1974--1976 an operation for adenoma removal was performed. 115 patients (44.2%) had a permanently placed catheter or were repeatedly catheterized before operation, and 145 (55.8%) were able to urinate without any need for catheterization. In the group of preoperatively catheterized patients infection of the urinary system was demonstrated in 84 cases, which amounts to 75%. Among patients not catheterized before operation, infection already existed in 29 cases (20%). Operation by Millin's method was carried out in 181 patients; non-infected urine was postoperatively found in 40 cases, which amounts to 22.3%. 79 patients were operated on by the transvesical method; in this group non-infected urine was found in four cases, i.e. 5.1%. Calculated x2 indicated a significant connection between the investigated items, consisting in a much more frequent occurrence of urinary system infection in patients operated on by the transvesical method. The duration of catheter reposition in the urinary bladder after prostatectomy considerably influences the number of postoperative infections of the urinary system."}
{"id": "PMID:468486", "title": "Polyps of the urethra in children.", "content": "The authors present two cases of polyps of the urethra occurring exclusively in boys: one in a 12-month-old infant, the other in a 15-year-old boy. In both patients the polyp caused dysuria or urinary retention. Diagnosis was based on mictional cystography. This examination usually gives a picture of a filling defect spotted in the area of the bladder neck. During miction the defect may move deeper into the urethra. An endoscopic examination may be also helpful. The authors believe that treatment should consist in total excision of the polyp together with its base through the open urinary bladder. Polyps of the urethra have to be differentiated from a valve of the posterior urethra or a shadowless calculus. After total excision of the polyp prognosis is good.", "contents": "Polyps of the urethra in children. The authors present two cases of polyps of the urethra occurring exclusively in boys: one in a 12-month-old infant, the other in a 15-year-old boy. In both patients the polyp caused dysuria or urinary retention. Diagnosis was based on mictional cystography. This examination usually gives a picture of a filling defect spotted in the area of the bladder neck. During miction the defect may move deeper into the urethra. An endoscopic examination may be also helpful. The authors believe that treatment should consist in total excision of the polyp together with its base through the open urinary bladder. Polyps of the urethra have to be differentiated from a valve of the posterior urethra or a shadowless calculus. After total excision of the polyp prognosis is good."}
{"id": "PMID:468487", "title": "Roentgenological investigations of the urethra in the dianosis of prostatic cancer.", "content": "The reliability of roentgenological demonstration of the urethra in prostatic cancer has been examined and the advantages of double contrast retrograde urethrocystography and resistance miction-cystourethrography are accentuated. These investigations are indicated in preparation for radiotherapy, operative therapy, transurethral resection and after reduced uroflow.", "contents": "Roentgenological investigations of the urethra in the dianosis of prostatic cancer. The reliability of roentgenological demonstration of the urethra in prostatic cancer has been examined and the advantages of double contrast retrograde urethrocystography and resistance miction-cystourethrography are accentuated. These investigations are indicated in preparation for radiotherapy, operative therapy, transurethral resection and after reduced uroflow."}
{"id": "PMID:468488", "title": "Operative blood loss during common urological operations.", "content": "The knowledge of mean blood loss during common urological procedures may be of some help for the surgeon in the appraisal of the magnitude of bleeding and planning blood transfusion. In 9 types of common urological operations (160 patients) the operative blood loss was determined. Results were mathematically evaluated.", "contents": "Operative blood loss during common urological operations. The knowledge of mean blood loss during common urological procedures may be of some help for the surgeon in the appraisal of the magnitude of bleeding and planning blood transfusion. In 9 types of common urological operations (160 patients) the operative blood loss was determined. Results were mathematically evaluated."}
{"id": "PMID:468489", "title": "Replacement of the ureter by means of a narrowed terminal ileal segment in one stage.", "content": "A proximally hypoplastic ureter causing hydronephrosis was replaced by a tightened ileal segment. The operation was performed in one stage and proved to be successful 10 years later.", "contents": "Replacement of the ureter by means of a narrowed terminal ileal segment in one stage. A proximally hypoplastic ureter causing hydronephrosis was replaced by a tightened ileal segment. The operation was performed in one stage and proved to be successful 10 years later."}
{"id": "PMID:468490", "title": "Measurement of total-body 40potassium in patients with urate calculi.", "content": "Fifty-eight patients with urate calculi and 31 patients with other (non-urate) calculi were examined for total-body potassium by means of a total-body counter. The patients with urate calculosis were found to differ significantly in their total-body K from patients with other calculi as well as from normal subjects. The results indicate that total-body potassium deficiency is one of the factors causing urate calculosis.", "contents": "Measurement of total-body 40potassium in patients with urate calculi. Fifty-eight patients with urate calculi and 31 patients with other (non-urate) calculi were examined for total-body potassium by means of a total-body counter. The patients with urate calculosis were found to differ significantly in their total-body K from patients with other calculi as well as from normal subjects. The results indicate that total-body potassium deficiency is one of the factors causing urate calculosis."}
{"id": "PMID:468491", "title": "Effects of experimental embolization of superior mesenteric artery branch on the intestine.", "content": "A primary mesenteric branch of the superior mesenteric artery was embolized with Amicar-mixed clots in seven dogs and with gelatin sponges in five dogs. All of the dogs embolized with Amicar-mixed clots survived the experimental period and no mesenteric damage was found with postmortem studies. Three of the five dogs embolized with gelatin sponges showed a local stricture within the embolized segment of intestine. Collateral blood supply to the strictured segment of bowel in these three dogs developed via arterial arcades, mural branches of the vasa recta and omental arteries.", "contents": "Effects of experimental embolization of superior mesenteric artery branch on the intestine. A primary mesenteric branch of the superior mesenteric artery was embolized with Amicar-mixed clots in seven dogs and with gelatin sponges in five dogs. All of the dogs embolized with Amicar-mixed clots survived the experimental period and no mesenteric damage was found with postmortem studies. Three of the five dogs embolized with gelatin sponges showed a local stricture within the embolized segment of intestine. Collateral blood supply to the strictured segment of bowel in these three dogs developed via arterial arcades, mural branches of the vasa recta and omental arteries."}
{"id": "PMID:468492", "title": "The radiologic assessment of vessel wall thickness.", "content": "A method allowing accurate and repeated measurements of vessel wall thickness has been developed. It consists of permanent opacification of the outer wall with tantalum in conjunction with magnification angiography. The technique was tested in a model, in human saphenous vein specimens, and in long-term experiments in rabbit blood vessels. Eighteen rabbits underwent exploratory surgery, after which the abdominal aorta, inferior vena cave, and the femoral and carotid arteries were coated with tantalum paste. Subsequently, magnification angiograms were performed from one to 14 months later. The wall thickness ranging between 100 and 300 micron was clearly visualized. While no gross inflammatory or foreign body reaction was seen, small granulomata adjacent to the blood vessels were frequently identified. The method described can be valuable in studying the natural history of the atherosclerotic process, particularly the behavior of vascular grafts.", "contents": "The radiologic assessment of vessel wall thickness. A method allowing accurate and repeated measurements of vessel wall thickness has been developed. It consists of permanent opacification of the outer wall with tantalum in conjunction with magnification angiography. The technique was tested in a model, in human saphenous vein specimens, and in long-term experiments in rabbit blood vessels. Eighteen rabbits underwent exploratory surgery, after which the abdominal aorta, inferior vena cave, and the femoral and carotid arteries were coated with tantalum paste. Subsequently, magnification angiograms were performed from one to 14 months later. The wall thickness ranging between 100 and 300 micron was clearly visualized. While no gross inflammatory or foreign body reaction was seen, small granulomata adjacent to the blood vessels were frequently identified. The method described can be valuable in studying the natural history of the atherosclerotic process, particularly the behavior of vascular grafts."}
{"id": "PMID:468493", "title": "A comparison of the cardiovascular responses to carotid injections of ionic and nonionic contrast media.", "content": "Selective injection of contrast media into the canine common carotid artery results in alterations in carotid flow, heart rate, and systemic arterial pressure. In this study the responses to injections of the nonionic agents metrizamide and iopamidol were compared to the responses to the ionic agents meglumine iothalamate, meglumine sodium diatrizoate, mannitol, and saline solution. Heart rate and pressure responses were smallest for metrizamide, greater for iopamidol, and greater still for the hypertonic ionic agents: responses were roughly correlated to agent osmolality. Carotid flow increased with all agents and was roughly proportional to the agent's osmolality. The exception was metrizamide which despite its lowest osmolality, produced a large increase in flow which was maintained much longer than for other agents. The total response to the nonionic agents was judged less severe than to the ionic agents; however, the responses to all agents were transient and not life threatening.", "contents": "A comparison of the cardiovascular responses to carotid injections of ionic and nonionic contrast media. Selective injection of contrast media into the canine common carotid artery results in alterations in carotid flow, heart rate, and systemic arterial pressure. In this study the responses to injections of the nonionic agents metrizamide and iopamidol were compared to the responses to the ionic agents meglumine iothalamate, meglumine sodium diatrizoate, mannitol, and saline solution. Heart rate and pressure responses were smallest for metrizamide, greater for iopamidol, and greater still for the hypertonic ionic agents: responses were roughly correlated to agent osmolality. Carotid flow increased with all agents and was roughly proportional to the agent's osmolality. The exception was metrizamide which despite its lowest osmolality, produced a large increase in flow which was maintained much longer than for other agents. The total response to the nonionic agents was judged less severe than to the ionic agents; however, the responses to all agents were transient and not life threatening."}
{"id": "PMID:468494", "title": "Renal excretion mechanism of a nonionic contrast agent.", "content": "The renal excretion mechanism of a nonionic experimental contrast agent, C-29, was investigated in rabbits in comparison with the ionic contrast agent sodium diatrizoate under conditions of free urine flow and ureteral stasis. The two contrast agents were injected as a mixture of 125I-C-29 and 131I-sodium diatrizoate, in doses of 25 and 250 mg I/kg. The results indicate that, during the period relevant for urographic examinations, C-29 may have a renal clearance as low as 0.7 times that of sodium diatrizoate under conditions of both free urine flow and ureteral stasis. The biologic half-life of C-29 is 41-50 min.", "contents": "Renal excretion mechanism of a nonionic contrast agent. The renal excretion mechanism of a nonionic experimental contrast agent, C-29, was investigated in rabbits in comparison with the ionic contrast agent sodium diatrizoate under conditions of free urine flow and ureteral stasis. The two contrast agents were injected as a mixture of 125I-C-29 and 131I-sodium diatrizoate, in doses of 25 and 250 mg I/kg. The results indicate that, during the period relevant for urographic examinations, C-29 may have a renal clearance as low as 0.7 times that of sodium diatrizoate under conditions of both free urine flow and ureteral stasis. The biologic half-life of C-29 is 41-50 min."}
{"id": "PMID:468495", "title": "The influence of fasting on opacification of the gallbladder during oral cholecystography using two consecutive doses in a canine model.", "content": "Oral cholecystography was performed in eight adult mongrel dogs. Contrast agents (Telepaque and/or Bilopaque) were administered in six different combinations, given as two consecutive doses with and without food. A combination of Bilopaque alone on both days and a combination of Telepaque with food on the first day and Bilopaque alone on the second day each proved to be the best method in 37.5% of the dogs. Telepaque with food on both consecutive days was the best method in 25% of the dogs. Among the eight combinations, two consecutive doses of Bilopaque alone or a combination of Telepaque with food on the first day and Bilopaque alone on the second day resulted in the most dense opacification of the gallbladder. Clinical implications of these results are discussed.", "contents": "The influence of fasting on opacification of the gallbladder during oral cholecystography using two consecutive doses in a canine model. Oral cholecystography was performed in eight adult mongrel dogs. Contrast agents (Telepaque and/or Bilopaque) were administered in six different combinations, given as two consecutive doses with and without food. A combination of Bilopaque alone on both days and a combination of Telepaque with food on the first day and Bilopaque alone on the second day each proved to be the best method in 37.5% of the dogs. Telepaque with food on both consecutive days was the best method in 25% of the dogs. Among the eight combinations, two consecutive doses of Bilopaque alone or a combination of Telepaque with food on the first day and Bilopaque alone on the second day resulted in the most dense opacification of the gallbladder. Clinical implications of these results are discussed."}
{"id": "PMID:468496", "title": "Effects of intracoronary administration of contrast materials on left ventricular dimensions and rate of relaxation.", "content": "The effects of intracoronary administration of ionic and nonionic contrast materials on (LV)left ventricular contractile state, relaxation rate (peak -dp/dt and [-dp/dt]50), and dimensions were determined in eight morphine-chloralose anesthetized dogs. Meglumine sodium diatrizoate caused decreases in peak dp/dt and -dp/dt and increases in left ventricular end systolic dimension (LVESD'), end diastolic dimension (LVEDD'), and end diastolic pressure(LVEDP). The decreases in peak -dp/dt and(-dp/dt)50 persisted after peak dp/dt returned to and exceeded control levels. Calcium meglumine sodium metrizoate caused increases in peak dp/dt but decreases in peak -dp/dt and(-dp/dt)50 and no significant changes in LVEDP, LVESD', or LVED'D. Metrizamide caused no significant changes. Ionic contrast materials induce important changes in LV dimensions and rate of relaxation in addition to producing a depression in contractile state. The decreases in  relaxation rate are not reversed by addition of calcium ions.", "contents": "Effects of intracoronary administration of contrast materials on left ventricular dimensions and rate of relaxation. The effects of intracoronary administration of ionic and nonionic contrast materials on (LV)left ventricular contractile state, relaxation rate (peak -dp/dt and [-dp/dt]50), and dimensions were determined in eight morphine-chloralose anesthetized dogs. Meglumine sodium diatrizoate caused decreases in peak dp/dt and -dp/dt and increases in left ventricular end systolic dimension (LVESD'), end diastolic dimension (LVEDD'), and end diastolic pressure(LVEDP). The decreases in peak -dp/dt and(-dp/dt)50 persisted after peak dp/dt returned to and exceeded control levels. Calcium meglumine sodium metrizoate caused increases in peak dp/dt but decreases in peak -dp/dt and(-dp/dt)50 and no significant changes in LVEDP, LVESD', or LVED'D. Metrizamide caused no significant changes. Ionic contrast materials induce important changes in LV dimensions and rate of relaxation in addition to producing a depression in contractile state. The decreases in  relaxation rate are not reversed by addition of calcium ions."}
{"id": "PMID:468498", "title": "In vivo measurement of absolute tissue iodine content by fluorescence excitation.", "content": "A method based on the measurement of fluorescent and transmitted photons has been developed for the quantitative determination of stable iodine concentrations in tissues. The results are independent of the thickness of the material surrounding the measuring site. The method yields an accuracy of better than 3% for both Ge semiconductor and NaI(TI)detector arrangements.", "contents": "In vivo measurement of absolute tissue iodine content by fluorescence excitation. A method based on the measurement of fluorescent and transmitted photons has been developed for the quantitative determination of stable iodine concentrations in tissues. The results are independent of the thickness of the material surrounding the measuring site. The method yields an accuracy of better than 3% for both Ge semiconductor and NaI(TI)detector arrangements."}
{"id": "PMID:468497", "title": "Sequential changes in the CT numbers of the normal canine kidney following intravenous contrast administration. II: The renal medulla.", "content": "Following the intravenous administration of sodium and meglumine salts of both diatrizoate and iothalamate (at doses of 310-880 mg I/kg body weight) sequential changes in computed tomographic (CT) numbers of the inner medulla were determined in three dogs, using a 30-second CT scanner. Peak medullary enhancement was greater than, and usually occurred 1-2 minutes after, peak cortical enhancement. The CT number of the medulla increased linearly with increasingly large doses of the contrast agent. At peak enhancement, the meglumine salts produced a significantly lower (p less than 0.025) mean CT attenuation value of the medulla than did the sodium salts. The difference in mean attenuation values between the sodium salts and meglumine salts was maximum (50-60 HU)at peak enhancement. Our data indicate the known difference in renal handling of the meglumine and sodium salts is readily detectable by CT.", "contents": "Sequential changes in the CT numbers of the normal canine kidney following intravenous contrast administration. II: The renal medulla. Following the intravenous administration of sodium and meglumine salts of both diatrizoate and iothalamate (at doses of 310-880 mg I/kg body weight) sequential changes in computed tomographic (CT) numbers of the inner medulla were determined in three dogs, using a 30-second CT scanner. Peak medullary enhancement was greater than, and usually occurred 1-2 minutes after, peak cortical enhancement. The CT number of the medulla increased linearly with increasingly large doses of the contrast agent. At peak enhancement, the meglumine salts produced a significantly lower (p less than 0.025) mean CT attenuation value of the medulla than did the sodium salts. The difference in mean attenuation values between the sodium salts and meglumine salts was maximum (50-60 HU)at peak enhancement. Our data indicate the known difference in renal handling of the meglumine and sodium salts is readily detectable by CT."}
{"id": "PMID:468499", "title": "Regional cerebral mode transit time and the derivative of the regional cerebral function recorded for intravenous administration of a non-diffusible tracer.", "content": "The meaning of the time from origin to first peak and from peak to peak of the derivative of the regional cerebral functions recorded after intravenous administration of a non-diffusible tracer is analyzed. Data obtained by cerebral and cardiac monitoring and evaluation of both times in patients with carotid thrombosis, indicates that the time from origin to first peak may be a better estimate of regional cerebral mode transit time than the time from peak to peak.", "contents": "Regional cerebral mode transit time and the derivative of the regional cerebral function recorded for intravenous administration of a non-diffusible tracer. The meaning of the time from origin to first peak and from peak to peak of the derivative of the regional cerebral functions recorded after intravenous administration of a non-diffusible tracer is analyzed. Data obtained by cerebral and cardiac monitoring and evaluation of both times in patients with carotid thrombosis, indicates that the time from origin to first peak may be a better estimate of regional cerebral mode transit time than the time from peak to peak."}
{"id": "PMID:468509", "title": "Assessment of pyeloureteral function using a flow velocity and cross-sectional diameter probe.", "content": "The response of the healthy pyeloureteral system of the pig to stepwise increments of urine flow was measured using a flow-velocity, diameter probe. The following urodynamic parameters were measured: renal pelvic pressure, ureteral flow-velocity and diameter, and associated bolus volume in the range of urine flow rates of 0.3 to 10 ml per min. The results show that the diameter sensoring portion of this probe can reliably sense ureteral peristalsis over the entire range of urine flow rates; small alterations in ureteral diameter occurring in dilated ureters after high diuretic loads can also be detected. Data obtained using the flow-velocity portion of this probe indicate that at urine flow rates less than 0.5 ml per min flow-velocity changes of a bolus can be reliably identified. At these flow rates the maximum flow-velocity occurs at the leading edge of the bolus and is invariant with the size of the bolus. At urine flow rates more than 1 ml per min the flow-velocity curve is polyphasic, indicative of an alteration in the mechanism of urine transport by the ureter at these flows. Collectively, data obtained using this probe provide dynamic information concerning the physiologic properties of the upper urinary tract not currently attainable in existing pressure/flow methodologies.", "contents": "Assessment of pyeloureteral function using a flow velocity and cross-sectional diameter probe. The response of the healthy pyeloureteral system of the pig to stepwise increments of urine flow was measured using a flow-velocity, diameter probe. The following urodynamic parameters were measured: renal pelvic pressure, ureteral flow-velocity and diameter, and associated bolus volume in the range of urine flow rates of 0.3 to 10 ml per min. The results show that the diameter sensoring portion of this probe can reliably sense ureteral peristalsis over the entire range of urine flow rates; small alterations in ureteral diameter occurring in dilated ureters after high diuretic loads can also be detected. Data obtained using the flow-velocity portion of this probe indicate that at urine flow rates less than 0.5 ml per min flow-velocity changes of a bolus can be reliably identified. At these flow rates the maximum flow-velocity occurs at the leading edge of the bolus and is invariant with the size of the bolus. At urine flow rates more than 1 ml per min the flow-velocity curve is polyphasic, indicative of an alteration in the mechanism of urine transport by the ureter at these flows. Collectively, data obtained using this probe provide dynamic information concerning the physiologic properties of the upper urinary tract not currently attainable in existing pressure/flow methodologies."}
{"id": "PMID:468511", "title": "Sacral root stimulation in controlled micturition. Peripheral somatic neurotomy and stimulated voiding.", "content": "Stimulation of the sacral ventral roots with the largest detrusor representation was found to be moderately successful for bladder emptying in acute canine experiments. However, removal of spinal reflexes arising from sacral root stimulation did not eliminate sphincteric resistance. For that reason ventral roots were stimulated after peripheral somatic neurotomy. Unilateral pudendal nerve sectioning was compared with selective sectioning of the somatic fibers of S2, the root with the greatest detrusor representation in canines. Both were equally effective in significantly reducing outlet resistance. Selective nerve section has one advantage: it preserves more of the nerve pathways involved in reflexogenic erection.", "contents": "Sacral root stimulation in controlled micturition. Peripheral somatic neurotomy and stimulated voiding. Stimulation of the sacral ventral roots with the largest detrusor representation was found to be moderately successful for bladder emptying in acute canine experiments. However, removal of spinal reflexes arising from sacral root stimulation did not eliminate sphincteric resistance. For that reason ventral roots were stimulated after peripheral somatic neurotomy. Unilateral pudendal nerve sectioning was compared with selective sectioning of the somatic fibers of S2, the root with the greatest detrusor representation in canines. Both were equally effective in significantly reducing outlet resistance. Selective nerve section has one advantage: it preserves more of the nerve pathways involved in reflexogenic erection."}
{"id": "PMID:468510", "title": "Studies on lactic dehydrogenase of patients with urinary bladder tumors. I. Urinary lactic dehydrogenase.", "content": "Urinary lactic dehydrogenase (LDH) activity and isozyme pattern were investigated in 38 patients with bladder tumors of various stages and grades and in 10 healthy persons. Urinary LDH activity was markedly elevated and directly correlated with histologic stage and grade of tumors. The urinary LDH isozyme pattern differed significantly from that of normal urine even in early and well-differentiated tumor cases. LDH4 and LDH5 became more prominent as the degree of the histologic stage and grade of the tumor increased. In the patients with tumors more advanced than stage T2, LDH4 and LDH5 were greater than LDH1 plus LDH2, the reverse of what is found in normal urine. As analogous result was obtained in patients with tumors of grade II or higher. No appreciable difference was found in the LDH isozyme pattern of patients with single or multiple tumors although the total activity was greater with multiple tumors. A comparison between LDH isozyme pattern in urine and in tumor tissue was evaluated in 26 patients with a good correlation (r = 0.40, P less than 0.05). Comparison of the results with urine LDH and urine cytology was attempted for each stage and grade. In 5 of 16 cases having negative urine cytology, a positive isozyme index was present and 10 of 13 cases having positive urine cytology had a positive isozyme index. These data suggest that evaluation of urinary LDH combined with urinary cytology might be of value in the diagnosis of bladder tumors and for following patients who have had bladder tumors.", "contents": "Studies on lactic dehydrogenase of patients with urinary bladder tumors. I. Urinary lactic dehydrogenase. Urinary lactic dehydrogenase (LDH) activity and isozyme pattern were investigated in 38 patients with bladder tumors of various stages and grades and in 10 healthy persons. Urinary LDH activity was markedly elevated and directly correlated with histologic stage and grade of tumors. The urinary LDH isozyme pattern differed significantly from that of normal urine even in early and well-differentiated tumor cases. LDH4 and LDH5 became more prominent as the degree of the histologic stage and grade of the tumor increased. In the patients with tumors more advanced than stage T2, LDH4 and LDH5 were greater than LDH1 plus LDH2, the reverse of what is found in normal urine. As analogous result was obtained in patients with tumors of grade II or higher. No appreciable difference was found in the LDH isozyme pattern of patients with single or multiple tumors although the total activity was greater with multiple tumors. A comparison between LDH isozyme pattern in urine and in tumor tissue was evaluated in 26 patients with a good correlation (r = 0.40, P less than 0.05). Comparison of the results with urine LDH and urine cytology was attempted for each stage and grade. In 5 of 16 cases having negative urine cytology, a positive isozyme index was present and 10 of 13 cases having positive urine cytology had a positive isozyme index. These data suggest that evaluation of urinary LDH combined with urinary cytology might be of value in the diagnosis of bladder tumors and for following patients who have had bladder tumors."}
{"id": "PMID:468512", "title": "Partial inhibition of castration-induced involution in rat prostate by chloroquine. A preliminary observation.", "content": "The effect of chloroquine phosphate, a membrane stabilizing agent, on castration-induced involution in the prostate was investigated in adult Sprague-Dawley rats. Chloroquine phosphate (75 mg per kg of body weight) was administered daily by gastric tube on 4 consecutive days beginning 1 day before castration. Control rats received water. All animals were sacrificed 7 days after castration and the ventral prostates were analyzed. The chloroquine group had a mean prostatic weight 17 per cent greater than that of the water-fed control group (P less than 0.01) despite a modest loss in body weight. The activity of cathepsin D, a lysosomal enzyme, in the prostate of treated rats was double that measured in control rats. Histologically, prostates from chloroquine treated rats contained more lysosomal particles that were larger than those from control rats. Serum testosterone reached castrate levels in both groups of animals within 24 hr of castration. These results indicate that it is possible to reduce the rate of prostatic regression by chloroquine, although at a small magnitude, probably through the action of membrane stabilization.", "contents": "Partial inhibition of castration-induced involution in rat prostate by chloroquine. A preliminary observation. The effect of chloroquine phosphate, a membrane stabilizing agent, on castration-induced involution in the prostate was investigated in adult Sprague-Dawley rats. Chloroquine phosphate (75 mg per kg of body weight) was administered daily by gastric tube on 4 consecutive days beginning 1 day before castration. Control rats received water. All animals were sacrificed 7 days after castration and the ventral prostates were analyzed. The chloroquine group had a mean prostatic weight 17 per cent greater than that of the water-fed control group (P less than 0.01) despite a modest loss in body weight. The activity of cathepsin D, a lysosomal enzyme, in the prostate of treated rats was double that measured in control rats. Histologically, prostates from chloroquine treated rats contained more lysosomal particles that were larger than those from control rats. Serum testosterone reached castrate levels in both groups of animals within 24 hr of castration. These results indicate that it is possible to reduce the rate of prostatic regression by chloroquine, although at a small magnitude, probably through the action of membrane stabilization."}
{"id": "PMID:468513", "title": "Attenuation by monosodium urate of the inhibitory effect of glycosaminoglycans on calcium oxalate nucleation.", "content": "The interaction between mucopolysaccharide (glycosaminoglycans) and monosodium urate, with respect to their effects on spontaneous precipitation of calcium oxalate, was explored in vitro. Heparin and, to a lesser extent, chondroitin sulfate were found to inhibit calcium oxalate precipitation. The formation product ratio (FPR, limit of metastability or the minimum supersaturation required for spontaneous nucleation) of calcium oxalate was increased 29 per cent by 0.05 mg of heparin per liter, and by 70 per cent with 1 mg of heparin per liter. The increment produced by heparin was much less pronounced when the solution containing the mucopolysaccharide was preincubated with crystalline monosodium urate. Pretreatment of the solution containing heparin (0.05 mg per liter) by monosodium urate (0.5 mg per ml) virtually eliminated the inhibitory action of heparin. Studies with radioactive heparin indicated that monosodium urate binds heparin. In contrast, the inhibitory effect of chondroitin sulfate was unaltered by prior exposure to monosodium urate. The results indicated that monosodium urate may attenuate the inhibitor activity of certain mucopolysaccharides such as heparin and facilitate the calcium oxalate precipitation. The findings provide further support for the important etiologic role of hyperuricosuria in the formation of calcium oxalate stones.", "contents": "Attenuation by monosodium urate of the inhibitory effect of glycosaminoglycans on calcium oxalate nucleation. The interaction between mucopolysaccharide (glycosaminoglycans) and monosodium urate, with respect to their effects on spontaneous precipitation of calcium oxalate, was explored in vitro. Heparin and, to a lesser extent, chondroitin sulfate were found to inhibit calcium oxalate precipitation. The formation product ratio (FPR, limit of metastability or the minimum supersaturation required for spontaneous nucleation) of calcium oxalate was increased 29 per cent by 0.05 mg of heparin per liter, and by 70 per cent with 1 mg of heparin per liter. The increment produced by heparin was much less pronounced when the solution containing the mucopolysaccharide was preincubated with crystalline monosodium urate. Pretreatment of the solution containing heparin (0.05 mg per liter) by monosodium urate (0.5 mg per ml) virtually eliminated the inhibitory action of heparin. Studies with radioactive heparin indicated that monosodium urate binds heparin. In contrast, the inhibitory effect of chondroitin sulfate was unaltered by prior exposure to monosodium urate. The results indicated that monosodium urate may attenuate the inhibitor activity of certain mucopolysaccharides such as heparin and facilitate the calcium oxalate precipitation. The findings provide further support for the important etiologic role of hyperuricosuria in the formation of calcium oxalate stones."}
{"id": "PMID:468514", "title": "Effect of parathyroidectomy on crystallization of calcium salts in urine of patients with primary hyperparathyroidism.", "content": "The effect of parathyroidectomy on the crystallization of calcium salts in urine was examined in seven stone-forming patients with primary hyperparathyroidism. After parathyroidectomy, urinary calcium decreased significantly from 205 +/- 30 to 67 +/- 11 mg per day (P less than 0.01); there were no significant changes in urinary phosphorus, oxalate, magnesium, sodium, potassium, uric acid, pH, or total volume. The urinary activity product ratio (state of saturation) of brushite (CaHPO4.2H2O) and calcium oxalate decreased significantly from 1.34 +/- 0.14 to 0.75 +/- 0.18 and from 3.20 +/- 0.56 to 1.53 +/- 0.21 respectively (P less than 0.05), owing principally to the decline in urinary calcium. Moreover, the urinary formation product ratio of calcium oxalate, which reflects the minimum supersaturation required for spontaneous nucleation, increased significantly after parathyroidectomy, from 7.19 +/- 1.19 to 12.99 +/- 1.69 (P less than 0.001). The results indicate that parathyroidectomy restores the normal urinary environment with respect to saturation and inhibitor and/or promoter activity.", "contents": "Effect of parathyroidectomy on crystallization of calcium salts in urine of patients with primary hyperparathyroidism. The effect of parathyroidectomy on the crystallization of calcium salts in urine was examined in seven stone-forming patients with primary hyperparathyroidism. After parathyroidectomy, urinary calcium decreased significantly from 205 +/- 30 to 67 +/- 11 mg per day (P less than 0.01); there were no significant changes in urinary phosphorus, oxalate, magnesium, sodium, potassium, uric acid, pH, or total volume. The urinary activity product ratio (state of saturation) of brushite (CaHPO4.2H2O) and calcium oxalate decreased significantly from 1.34 +/- 0.14 to 0.75 +/- 0.18 and from 3.20 +/- 0.56 to 1.53 +/- 0.21 respectively (P less than 0.05), owing principally to the decline in urinary calcium. Moreover, the urinary formation product ratio of calcium oxalate, which reflects the minimum supersaturation required for spontaneous nucleation, increased significantly after parathyroidectomy, from 7.19 +/- 1.19 to 12.99 +/- 1.69 (P less than 0.001). The results indicate that parathyroidectomy restores the normal urinary environment with respect to saturation and inhibitor and/or promoter activity."}
{"id": "PMID:468515", "title": "Rosoxacin and cinoxacin distribution in prostate, vagina, and female urethra. An experimental study in dogs.", "content": "Rosoxacin and cinoxacin, two new compounds structurally related to nalidixic acid, were investigated in dogs. Our primary interest was in the beneficial effects of the two drugs when used in the treatment of urinary tract infections. The distribution of rosoxacin and cinoxacin was investigated in prostatic tissue, prostatic interstitial fluid, and prostatic secretion, and in vaginal and female urethral secretions. Concentrations of both antibiotics were also measured in plasma, various tissues, and spinal fluid. The amounts of rosoxacin and cinoxacin in all tested tissues, fluids, and secretions were in the range of the minimal inhibitory concentrations for most Gram negative organisms found in urinary tract infections. This suggested possible beneficial effects of these drugs when the infection involves the above tissues. Rosoxacin concentration ratios were higher than those of cinoxacin relative to plasma concentration in prostatic, vaginal, and urethral secretions, as well as in prostatic and other tissue. These ratio differences were explained by the different physiochemical properties of the two drugs.", "contents": "Rosoxacin and cinoxacin distribution in prostate, vagina, and female urethra. An experimental study in dogs. Rosoxacin and cinoxacin, two new compounds structurally related to nalidixic acid, were investigated in dogs. Our primary interest was in the beneficial effects of the two drugs when used in the treatment of urinary tract infections. The distribution of rosoxacin and cinoxacin was investigated in prostatic tissue, prostatic interstitial fluid, and prostatic secretion, and in vaginal and female urethral secretions. Concentrations of both antibiotics were also measured in plasma, various tissues, and spinal fluid. The amounts of rosoxacin and cinoxacin in all tested tissues, fluids, and secretions were in the range of the minimal inhibitory concentrations for most Gram negative organisms found in urinary tract infections. This suggested possible beneficial effects of these drugs when the infection involves the above tissues. Rosoxacin concentration ratios were higher than those of cinoxacin relative to plasma concentration in prostatic, vaginal, and urethral secretions, as well as in prostatic and other tissue. These ratio differences were explained by the different physiochemical properties of the two drugs."}
{"id": "PMID:468516", "title": "Ureteral plication. A new concept in ureteral tailoring for megaureter.", "content": "Currently, the most favored surgical procedure used to restore normal peristalsis to massively dilated adynamic ureters is ureteral tailoring. This procedure, however, seriously jeopardizes the ureter's blood supply and frequently results in ischemia with ureteral stenosis and further renal deterioration. To preserve the distal ureter's vasculature a new technique of reducing ureteral caliber and restoring normal peristalsis with minimal disruption of the blood supply was developed and performed in 14 dogs with excellent results.", "contents": "Ureteral plication. A new concept in ureteral tailoring for megaureter. Currently, the most favored surgical procedure used to restore normal peristalsis to massively dilated adynamic ureters is ureteral tailoring. This procedure, however, seriously jeopardizes the ureter's blood supply and frequently results in ischemia with ureteral stenosis and further renal deterioration. To preserve the distal ureter's vasculature a new technique of reducing ureteral caliber and restoring normal peristalsis with minimal disruption of the blood supply was developed and performed in 14 dogs with excellent results."}
{"id": "PMID:468517", "title": "Analysis of renal calculi from a marine mollusc (Marcocallista nimbosa). Implications for the study of urolithiasis.", "content": "An X-ray diffraction analysis of kidney stones from the bivalved mollusc Macrocallista nimbosa has revealed the calculi composition to be amorhpous calcium phosphate. The use of this animal for the study of urolithiasis is suggested because of the spatial and temporal ubiquity of its renal calculi.", "contents": "Analysis of renal calculi from a marine mollusc (Marcocallista nimbosa). Implications for the study of urolithiasis. An X-ray diffraction analysis of kidney stones from the bivalved mollusc Macrocallista nimbosa has revealed the calculi composition to be amorhpous calcium phosphate. The use of this animal for the study of urolithiasis is suggested because of the spatial and temporal ubiquity of its renal calculi."}
{"id": "PMID:468518", "title": "Netilmicin and tobramycin. Comparison of nephrotoxicity in dogs.", "content": "We compared the effects of netilmicin and tobramycin on renal function and histology in dogs. Separate groups, each containing 5 dogs, received control injections, or either netilmicin or tobramycin at doses of 25, 50, or 75 mg per kg of body weight per day for 14 days. Renal function decreased markedly only in the group receiving the 75-mg tobramycin dosage; the serum creatinine levels rose from 1.0 +/- 0.1 to 3.6 +/- 0.9 mg per 100 ml (P less than 0.05) and the endogenous creatinine clearance fell from 42.5 +/- 9.4 to 7.8 +/- 2.2 ml per min (P less than 0.05). Dogs in this group developed glycosuria, proteinuria, and polyuria, and three died before the end of the study, probably from neuromuscular toxicity. Peak drug levels were stable when renal function was normal, but increased when renal failure occurred. Light microscopic changes occurred in all groups receiving either drug, but were most severe in the high-dose tobramycin group. Ultrastructural changes were similar in all groups and identical to changes produced by gentamicin. These results show that, on a weight basis, netilmicin is less nephrotoxic than tobramycin in dogs.", "contents": "Netilmicin and tobramycin. Comparison of nephrotoxicity in dogs. We compared the effects of netilmicin and tobramycin on renal function and histology in dogs. Separate groups, each containing 5 dogs, received control injections, or either netilmicin or tobramycin at doses of 25, 50, or 75 mg per kg of body weight per day for 14 days. Renal function decreased markedly only in the group receiving the 75-mg tobramycin dosage; the serum creatinine levels rose from 1.0 +/- 0.1 to 3.6 +/- 0.9 mg per 100 ml (P less than 0.05) and the endogenous creatinine clearance fell from 42.5 +/- 9.4 to 7.8 +/- 2.2 ml per min (P less than 0.05). Dogs in this group developed glycosuria, proteinuria, and polyuria, and three died before the end of the study, probably from neuromuscular toxicity. Peak drug levels were stable when renal function was normal, but increased when renal failure occurred. Light microscopic changes occurred in all groups receiving either drug, but were most severe in the high-dose tobramycin group. Ultrastructural changes were similar in all groups and identical to changes produced by gentamicin. These results show that, on a weight basis, netilmicin is less nephrotoxic than tobramycin in dogs."}
{"id": "PMID:468519", "title": "Absence of association between HLA antigens and primary open angle glaucoma in Israel.", "content": "HLA antigens of the A and B loci were determined in 57 Israeli patients with primary open angle glaucoma (POAG) presenting visual field loss and in 715 normal control subjects. The glaucoma patients and control subjects were non-related and randomly selected from the Jewish Israeli population. Frequencies of HLA antigens among the POAG patients showed no differences from those observed in the control subjects. Similar results were observed for all patients as well as within subgroups of the patient population as defined by origin. Thus, the association reported between B7 and/or B12 and POAG in white and black Americans could not be confirmed in an Israeli population.", "contents": "Absence of association between HLA antigens and primary open angle glaucoma in Israel. HLA antigens of the A and B loci were determined in 57 Israeli patients with primary open angle glaucoma (POAG) presenting visual field loss and in 715 normal control subjects. The glaucoma patients and control subjects were non-related and randomly selected from the Jewish Israeli population. Frequencies of HLA antigens among the POAG patients showed no differences from those observed in the control subjects. Similar results were observed for all patients as well as within subgroups of the patient population as defined by origin. Thus, the association reported between B7 and/or B12 and POAG in white and black Americans could not be confirmed in an Israeli population."}
{"id": "PMID:468520", "title": "Glucose production by the perfused liver of the spiny mouse (Acomys cahirinus): sensitivity to glucagon and insulin.", "content": "Hepatic glucose production and its responses to glucagon and insulin were studied in perfused livers obtained from spiny mice (Acomys cahirinus)--rodents characterized by normoglycemia, decreased glucose tolerance and decreased insulin secretion--and from Swiss albino mice. The dose-response characteristics of glucagon-induced hepatic glucose output were similar in spiny and albino mice. However, maximally stimulated glucose production were significantly lower in the spiny mouse. Furthermore, the liver of the spiny mouse was more sensitive to the action of insulin: 0.5 X 10(-6) M insulin significantly inhibited the action of glucagon in spiny mice but not in albino mice. It is proposed that the increased sensitivity of the hepatic glucose output to insulin and the decreased maximal stimulation by glucagon may be factors that limit the negative effects of the impaired insulin secretion in spiny mice, thereby reducing the severity of the glucose intolerance. Analogous mechanisms may be present in the early stages of the diabetic syndrome in man.", "contents": "Glucose production by the perfused liver of the spiny mouse (Acomys cahirinus): sensitivity to glucagon and insulin. Hepatic glucose production and its responses to glucagon and insulin were studied in perfused livers obtained from spiny mice (Acomys cahirinus)--rodents characterized by normoglycemia, decreased glucose tolerance and decreased insulin secretion--and from Swiss albino mice. The dose-response characteristics of glucagon-induced hepatic glucose output were similar in spiny and albino mice. However, maximally stimulated glucose production were significantly lower in the spiny mouse. Furthermore, the liver of the spiny mouse was more sensitive to the action of insulin: 0.5 X 10(-6) M insulin significantly inhibited the action of glucagon in spiny mice but not in albino mice. It is proposed that the increased sensitivity of the hepatic glucose output to insulin and the decreased maximal stimulation by glucagon may be factors that limit the negative effects of the impaired insulin secretion in spiny mice, thereby reducing the severity of the glucose intolerance. Analogous mechanisms may be present in the early stages of the diabetic syndrome in man."}
{"id": "PMID:468521", "title": "Effect of estradiol on erythropoiesis and megakaryocytopoiesis in mice.", "content": "The effect of four 1.0-mg estradiol benzoate injections on hemopoiesis in the spleen and bone marrow in mice was studied. The number, concentration and mitotic activity of megakaryocytes (MKC) and number of MKC colonies in the spleen were found to be increased one and two weeks after cessation of the estradiol injections. MKC concentration in the bone marrow rose during the experimental period. The MKC increased in number during the first week after estradiol, but dropped to normal after the second week. The number and mitotic activity of erythroid precursors increased in the bone marrow and spleen three days after the cessation of estradiol injections. These findings suggest that estrogen enhances megakaryocytopoiesis and erythropoiesis in the bone marrow and spleen in mice.", "contents": "Effect of estradiol on erythropoiesis and megakaryocytopoiesis in mice. The effect of four 1.0-mg estradiol benzoate injections on hemopoiesis in the spleen and bone marrow in mice was studied. The number, concentration and mitotic activity of megakaryocytes (MKC) and number of MKC colonies in the spleen were found to be increased one and two weeks after cessation of the estradiol injections. MKC concentration in the bone marrow rose during the experimental period. The MKC increased in number during the first week after estradiol, but dropped to normal after the second week. The number and mitotic activity of erythroid precursors increased in the bone marrow and spleen three days after the cessation of estradiol injections. These findings suggest that estrogen enhances megakaryocytopoiesis and erythropoiesis in the bone marrow and spleen in mice."}
{"id": "PMID:468522", "title": "Age-related changes in antibody-dependent cell-mediated cytotoxicity in mouse spleen.", "content": "The age-related variation in antibody-dependent cell-mediated cytotoxicity (ADCC) in spleen was examined in four different mouse strains (DBA/2, Balb/c, NZB and NZB/NZW). The ADCC effector activity against antibody-coated chicken red blood cells in untreated spleen from all four strains was roughly comparable. An initial rise in activity with increasing age until two to three months was followed by a sharp decline in effector activity. Treatment of the spleen cells with poly-l-lysine-coated carbonyl iron to remove phagocytes resulted in a decrease in ADCC activity in NZB, NZB/NZW and DBA/2 strains at all ages examined. The same general pattern of age-dependent decline was still seen in these depleted spleens. However, the same treatment essentially abolished ADCC in Balb/c spleen at all ages studied.", "contents": "Age-related changes in antibody-dependent cell-mediated cytotoxicity in mouse spleen. The age-related variation in antibody-dependent cell-mediated cytotoxicity (ADCC) in spleen was examined in four different mouse strains (DBA/2, Balb/c, NZB and NZB/NZW). The ADCC effector activity against antibody-coated chicken red blood cells in untreated spleen from all four strains was roughly comparable. An initial rise in activity with increasing age until two to three months was followed by a sharp decline in effector activity. Treatment of the spleen cells with poly-l-lysine-coated carbonyl iron to remove phagocytes resulted in a decrease in ADCC activity in NZB, NZB/NZW and DBA/2 strains at all ages examined. The same general pattern of age-dependent decline was still seen in these depleted spleens. However, the same treatment essentially abolished ADCC in Balb/c spleen at all ages studied."}
{"id": "PMID:468523", "title": "Medullary carcinoma of the thyroid.", "content": "Three patients with medullary carcinoma of the thyroid gland, one sporadic and two familial, are described. One of the patients presented at a late stage of the disease with evidence of wide dissemination and severe diarrhea, which was controlled by indomethacin. The value of venous catheterization and plasma thyrocalcitonin measurements in evaluating the extent of the disease was demonstrated in this case.", "contents": "Medullary carcinoma of the thyroid. Three patients with medullary carcinoma of the thyroid gland, one sporadic and two familial, are described. One of the patients presented at a late stage of the disease with evidence of wide dissemination and severe diarrhea, which was controlled by indomethacin. The value of venous catheterization and plasma thyrocalcitonin measurements in evaluating the extent of the disease was demonstrated in this case."}
{"id": "PMID:468524", "title": "Traumatic labyrinthine fistulas.", "content": "During the last 10 years, the rupture of the inner ear windows, as an entity, has been introduced in the differential diagnosis of the acute vestibulocochlear syndrome. Head trauma, Valsalva-like maneuvers and barotrauma due to diving accidents are now described as etiologic factors in the pathogenesis of labyrinthine fistula. Four cases were explored and repaired in the last four years in our department. Ataxia was the prominent symptom in three, while positional nystagmus and vertigo were consistent findings in all four cases. The definitive evidence of the fistula can be proved only by exploratory tympanotomy. In this report, the importance of the consideration of this entity in the differential diagnosis of vestibulocochlear syndrome in victims of traffic and diving accidents is emphasized.", "contents": "Traumatic labyrinthine fistulas. During the last 10 years, the rupture of the inner ear windows, as an entity, has been introduced in the differential diagnosis of the acute vestibulocochlear syndrome. Head trauma, Valsalva-like maneuvers and barotrauma due to diving accidents are now described as etiologic factors in the pathogenesis of labyrinthine fistula. Four cases were explored and repaired in the last four years in our department. Ataxia was the prominent symptom in three, while positional nystagmus and vertigo were consistent findings in all four cases. The definitive evidence of the fistula can be proved only by exploratory tympanotomy. In this report, the importance of the consideration of this entity in the differential diagnosis of vestibulocochlear syndrome in victims of traffic and diving accidents is emphasized."}
{"id": "PMID:468525", "title": "Acute myocardial infarction in a hemophiliac.", "content": "An acute myocardial infarction was observed in a 62-year-old patient with hemophilia A, as well as myxedema, hypertension, obesity, hypercholesterolemia and angina pectoris. The occurrence of myocardial infarction in hemophiliacs is rare, and, to the best of our knowledge, this patient represents the fourth documented case in the literature.", "contents": "Acute myocardial infarction in a hemophiliac. An acute myocardial infarction was observed in a 62-year-old patient with hemophilia A, as well as myxedema, hypertension, obesity, hypercholesterolemia and angina pectoris. The occurrence of myocardial infarction in hemophiliacs is rare, and, to the best of our knowledge, this patient represents the fourth documented case in the literature."}
{"id": "PMID:468526", "title": "Cat-scratch disease presenting as status epilepticus. A case report.", "content": "A 12-year-old Bedouin boy presented with sudden asymmetrical status epilepticus. The presence of a scalp wound, rubbery cervical lymphadenopathy and a history of contact with cats suggested the diagnosis of cat-scratch disease complicated by encephalopathy. The diagnosis was supported by histological examination of a cervical lymph node and a positive skin test with cat-scratch antigen. The rapid establishment of the diagnosis avoided the necessity of elaborate and complicated neuroradiological investigations.", "contents": "Cat-scratch disease presenting as status epilepticus. A case report. A 12-year-old Bedouin boy presented with sudden asymmetrical status epilepticus. The presence of a scalp wound, rubbery cervical lymphadenopathy and a history of contact with cats suggested the diagnosis of cat-scratch disease complicated by encephalopathy. The diagnosis was supported by histological examination of a cervical lymph node and a positive skin test with cat-scratch antigen. The rapid establishment of the diagnosis avoided the necessity of elaborate and complicated neuroradiological investigations."}
{"id": "PMID:468551", "title": "Estimates of preventive versus nonpreventive medical care demand in an HMO.", "content": "Multiple regression analysis is used to investigate whether medical services in a large HMO are distributed primarily on the basis of need and predisposing factors (such as health status, age and sex) or according to enabling characteristics (such as coinsurance and income) of the population. Equations are formulated to estimate the likelihood and volume of preventive visit demand, nonpreventive visit demand and hospital admissions for a sample of 3,892 individuals enrolled in the Kaiser Foundation Health Plan of Portland, Oregon. The results indicate that predisposing and need factors are the main determinants of nonpreventive visits and hospital utilization, while enabling characteristics are important determinants (along with age and education) of preventive utilization. There are marked differences in the impact of explanatory factors on utilization by dependents (children) versus nondependents (adults).", "contents": "Estimates of preventive versus nonpreventive medical care demand in an HMO. Multiple regression analysis is used to investigate whether medical services in a large HMO are distributed primarily on the basis of need and predisposing factors (such as health status, age and sex) or according to enabling characteristics (such as coinsurance and income) of the population. Equations are formulated to estimate the likelihood and volume of preventive visit demand, nonpreventive visit demand and hospital admissions for a sample of 3,892 individuals enrolled in the Kaiser Foundation Health Plan of Portland, Oregon. The results indicate that predisposing and need factors are the main determinants of nonpreventive visits and hospital utilization, while enabling characteristics are important determinants (along with age and education) of preventive utilization. There are marked differences in the impact of explanatory factors on utilization by dependents (children) versus nondependents (adults)."}
{"id": "PMID:468552", "title": "The effect of personal health care services on longevity in an economically advanced population.", "content": "This article is an attempt to assess the effect of personal health care on longevity. It is assumed throughout that personal health care has a differential impact on the various causes of death. Historical and cross-sectional comparisons of the relative incidence of certain causes of death can, under this assumption, provide some insight into the effectiveness of personal health care generally and may provide a gauge of the relative benefits of different personal health care delivery systems. Using the U.S. male population of 1964 as a reference point, it is estimated that the withdrawal of personal health services would result at the most in a decrease in average length of life from 66.9 to 60.5 years.", "contents": "The effect of personal health care services on longevity in an economically advanced population. This article is an attempt to assess the effect of personal health care on longevity. It is assumed throughout that personal health care has a differential impact on the various causes of death. Historical and cross-sectional comparisons of the relative incidence of certain causes of death can, under this assumption, provide some insight into the effectiveness of personal health care generally and may provide a gauge of the relative benefits of different personal health care delivery systems. Using the U.S. male population of 1964 as a reference point, it is estimated that the withdrawal of personal health services would result at the most in a decrease in average length of life from 66.9 to 60.5 years."}
{"id": "PMID:468553", "title": "Indexes of severity: conceptual development.", "content": "A discussion of severity index development is presented in relation to conceptual issues in index definition, analytic issues in index formulation and validation issues in index application. The CHOP index is discussed along with six severity indexes described in an earlier paper dealing with underlying concepts to illustrate the material presented. Replies are provided to specific questions raised in an accompanying paper discussing the Injury Severity Score. This conceptual material is presented to provide a foundation for severity index development, to suggest criteria to be used in their formulation and testing, and to identify analyses that can lead to the successful selection and application of an index for a defined purpose.", "contents": "Indexes of severity: conceptual development. A discussion of severity index development is presented in relation to conceptual issues in index definition, analytic issues in index formulation and validation issues in index application. The CHOP index is discussed along with six severity indexes described in an earlier paper dealing with underlying concepts to illustrate the material presented. Replies are provided to specific questions raised in an accompanying paper discussing the Injury Severity Score. This conceptual material is presented to provide a foundation for severity index development, to suggest criteria to be used in their formulation and testing, and to identify analyses that can lead to the successful selection and application of an index for a defined purpose."}
{"id": "PMID:468554", "title": "Indexes of severity: underlying concepts--a reply.", "content": "A recent paper on the concepts underlying six indexes of severity that have been proposed for health services research claimed to identify deficiencies in each. A close examination reveals that the criticisms are generally without substance, and that the claim that the indexes \"violate some of the principles implied by their formulation\" is in error. Most of the objections apparently stem from fundamental confusion concerning the use of such indexes. Two of the indexes in question, the Abbreviated Injury Scale AIS) and the Injury Severity Score (ISS), have been validated and are being used widely both in the United States and elsewhere as the principal descriptors of trauma in motor vehicle crashes. The use of these indexes has contributed significantly to motor vehicle crash research and to the improvements in vehicles and highways that are reducing the trauma resulting from such crashes.", "contents": "Indexes of severity: underlying concepts--a reply. A recent paper on the concepts underlying six indexes of severity that have been proposed for health services research claimed to identify deficiencies in each. A close examination reveals that the criticisms are generally without substance, and that the claim that the indexes \"violate some of the principles implied by their formulation\" is in error. Most of the objections apparently stem from fundamental confusion concerning the use of such indexes. Two of the indexes in question, the Abbreviated Injury Scale AIS) and the Injury Severity Score (ISS), have been validated and are being used widely both in the United States and elsewhere as the principal descriptors of trauma in motor vehicle crashes. The use of these indexes has contributed significantly to motor vehicle crash research and to the improvements in vehicles and highways that are reducing the trauma resulting from such crashes."}
{"id": "PMID:468555", "title": "An ambulatory care classification system: design, development and evaluation.", "content": "A medical classification system for coding a patient's reason for visit has been developed for use in the National Ambulatory Medical Care Survey and for ambulatory care settings. The code is developed in a modular structure and includes modules for Symptoms; Diseases; Diagnostic, Screening and Preventive Procedures; Therapeutic Procedures, Process Problems and Counseling; Injuries and Adverse Effects; Follow-ups for Test Results; and Administrative Reasons for Visits. The system was evaluated in coding tests, through review by medical societies representing the major specialties, and by other health care providers. An extensive coding index is available.", "contents": "An ambulatory care classification system: design, development and evaluation. A medical classification system for coding a patient's reason for visit has been developed for use in the National Ambulatory Medical Care Survey and for ambulatory care settings. The code is developed in a modular structure and includes modules for Symptoms; Diseases; Diagnostic, Screening and Preventive Procedures; Therapeutic Procedures, Process Problems and Counseling; Injuries and Adverse Effects; Follow-ups for Test Results; and Administrative Reasons for Visits. The system was evaluated in coding tests, through review by medical societies representing the major specialties, and by other health care providers. An extensive coding index is available."}
{"id": "PMID:468556", "title": "[Scanning electron microscopy study of autologous vein and fascia lata before and one year after implantation into the arterial blood stream].", "content": "The present communication deals with scanning electronmicroscopical changes of autologous vein and autologous fascia lata, which were used as arterial implants. We examined material, which could be removed during reoperation for vascular occlusion. After one year, the surface exposed to the blood stream shows a homogenous layer of fibrin. Whilst the elastic fibers of fascia lata-transplants have nearly completely disappeared, we can still find them in the vein. This might be the essential reason for the longer survival of venous implants into the arterial blood stream.", "contents": "[Scanning electron microscopy study of autologous vein and fascia lata before and one year after implantation into the arterial blood stream]. The present communication deals with scanning electronmicroscopical changes of autologous vein and autologous fascia lata, which were used as arterial implants. We examined material, which could be removed during reoperation for vascular occlusion. After one year, the surface exposed to the blood stream shows a homogenous layer of fibrin. Whilst the elastic fibers of fascia lata-transplants have nearly completely disappeared, we can still find them in the vein. This might be the essential reason for the longer survival of venous implants into the arterial blood stream."}
{"id": "PMID:468558", "title": "[Therapy of pleural empyema after pneumonectomy with open-window drainage and continuous rinsing].", "content": "From 1961 to 1977 24 thoracic empyemas (=4,75%) after 507 pneumonectomies were observed at the Surgical Clinic A of the Zurich University Hospital. Two methods for the management of this condition are discussed: open-window drainage (CLAGETT) and continuous rinsing of the pleural cavity (LUIZY). The first mentioned method proved to be a palliative one in our patients: no thoracostomy could be re-closed operatively. Two thoracic windows healed up spontaneously without recurrence of an empyema; one patient died shortly after the operation from respiratory insufficiency. Of the five patients treated by continuous rinsing, four were cured as for their empyema, but one of the latter died from renal insufficiency. In one case an open-window drainage finally had to be accomplished.", "contents": "[Therapy of pleural empyema after pneumonectomy with open-window drainage and continuous rinsing]. From 1961 to 1977 24 thoracic empyemas (=4,75%) after 507 pneumonectomies were observed at the Surgical Clinic A of the Zurich University Hospital. Two methods for the management of this condition are discussed: open-window drainage (CLAGETT) and continuous rinsing of the pleural cavity (LUIZY). The first mentioned method proved to be a palliative one in our patients: no thoracostomy could be re-closed operatively. Two thoracic windows healed up spontaneously without recurrence of an empyema; one patient died shortly after the operation from respiratory insufficiency. Of the five patients treated by continuous rinsing, four were cured as for their empyema, but one of the latter died from renal insufficiency. In one case an open-window drainage finally had to be accomplished."}
{"id": "PMID:468559", "title": "[Pacemaker implantation under local anesthesia].", "content": "102 pacemakers have been implanted under local anesthesia. The electrodes have been introduced through the left vena cephalica and the pacemakers placed in the infraclavicular groove. Exchanged pacemakers have been put in the already existing cavity. The wounds have been closed by sutures consisting solely of polyglycolic acid. One hematoma and one infection have been observed. In 12% of the cases the electrode was introduced through the vena jugularis externa, owing to obstruction or unsuitable size of the vena cephalica. Reoperation was necessary in 3 cases due to dislocation of the electrode, and in one case due to the perforation of the ventricle caused by the electrode.", "contents": "[Pacemaker implantation under local anesthesia]. 102 pacemakers have been implanted under local anesthesia. The electrodes have been introduced through the left vena cephalica and the pacemakers placed in the infraclavicular groove. Exchanged pacemakers have been put in the already existing cavity. The wounds have been closed by sutures consisting solely of polyglycolic acid. One hematoma and one infection have been observed. In 12% of the cases the electrode was introduced through the vena jugularis externa, owing to obstruction or unsuitable size of the vena cephalica. Reoperation was necessary in 3 cases due to dislocation of the electrode, and in one case due to the perforation of the ventricle caused by the electrode."}
{"id": "PMID:468560", "title": "[Experiences with the Sarmiento-cast].", "content": "The patella-tendon-bearing cast described by Sarmiento was used in the treatment of 96 tibial fractures. Full weight-bearing was permitted even if the fractures were completely unstable and had a marked tendency to shorten. Only in one case weight-bearing led to a dislocation of the fragments. Specific complications were not seen. It was evident that the PTB-cast was most advantageous to the patient in comparison with a conventional cast and sometimes even in comparison with an osteosynthesis, which does not tolerate weight-bearing. To integrate the Sarmiento-cast in the routine of a small or middle sized hospital, we recommend to observe the following rules: 1. The cast is not applied immediately after the accident, but after one to three weeks of conventional conservative treatment. 2. It is made by two specially trained doctors. 3. The controls of the out-patients are performed in the hospital and not delegated to the family doctor.", "contents": "[Experiences with the Sarmiento-cast]. The patella-tendon-bearing cast described by Sarmiento was used in the treatment of 96 tibial fractures. Full weight-bearing was permitted even if the fractures were completely unstable and had a marked tendency to shorten. Only in one case weight-bearing led to a dislocation of the fragments. Specific complications were not seen. It was evident that the PTB-cast was most advantageous to the patient in comparison with a conventional cast and sometimes even in comparison with an osteosynthesis, which does not tolerate weight-bearing. To integrate the Sarmiento-cast in the routine of a small or middle sized hospital, we recommend to observe the following rules: 1. The cast is not applied immediately after the accident, but after one to three weeks of conventional conservative treatment. 2. It is made by two specially trained doctors. 3. The controls of the out-patients are performed in the hospital and not delegated to the family doctor."}
{"id": "PMID:468561", "title": "[Management of traumatic rupture of the aorta].", "content": "Traumatic rupture of the thoracic aorta is a common injury in automobile accidents. It is the result of a crushing chest injury, most frequently associated with sudden deceleration. Approximately 10 to 20% of the individuals with traumatic rupture of the aorta survive temporarily. Diagnosis may be difficult, but if the lesion is promptly diagnosed, appropriate surgical treatment may be life-saving. 9 patients with traumatic aortic rupture have been operated on at our unit since 1969. 2 died postoperatively from acute respiratory distress syndrome. 7 survived the immediate postoperative period without major complications. 2 of them died later after four weeks because of complications not related to the operation. Based on the literature and on our own experience some directions in the management of the traumatic rupture of the thoracic aorta are presented.", "contents": "[Management of traumatic rupture of the aorta]. Traumatic rupture of the thoracic aorta is a common injury in automobile accidents. It is the result of a crushing chest injury, most frequently associated with sudden deceleration. Approximately 10 to 20% of the individuals with traumatic rupture of the aorta survive temporarily. Diagnosis may be difficult, but if the lesion is promptly diagnosed, appropriate surgical treatment may be life-saving. 9 patients with traumatic aortic rupture have been operated on at our unit since 1969. 2 died postoperatively from acute respiratory distress syndrome. 7 survived the immediate postoperative period without major complications. 2 of them died later after four weeks because of complications not related to the operation. Based on the literature and on our own experience some directions in the management of the traumatic rupture of the thoracic aorta are presented."}
{"id": "PMID:468562", "title": "[Functional and oncologic results after bronchial or blood vessel anastomosis in the resection therapy of bronchogenic carcinoma].", "content": "In treatment of bronchogenic carcinoma twelve selected patients had economical resections. To avoid a pneumonectomy, lobectomies or bilobectomies were associated twice with bronchial excision, 8 times with bronchial resection (sleeve resection) and twice with resection and anastomosis of pulmonary artery. Nine patients underwent clinical, radiologic and szintigraphic control. Except one case all patients showed good functional results. The anastomosed lobes were perfused and ventilated proportionally to the number of segments. The quality of life after partial pulmonary resections had deteriorated only to a small extent compared with the preoperative state. These results were emphasized by the only poor result which (after atelectasis of the anastomosed lung) corresponded functionally to a pneumonectomy. --The two cases of anastomosis of pulmonary artery showed a short survival. Both corresponded to a stage II of tumor classification. Of the other patients (all stage I) three are alive more than five years.", "contents": "[Functional and oncologic results after bronchial or blood vessel anastomosis in the resection therapy of bronchogenic carcinoma]. In treatment of bronchogenic carcinoma twelve selected patients had economical resections. To avoid a pneumonectomy, lobectomies or bilobectomies were associated twice with bronchial excision, 8 times with bronchial resection (sleeve resection) and twice with resection and anastomosis of pulmonary artery. Nine patients underwent clinical, radiologic and szintigraphic control. Except one case all patients showed good functional results. The anastomosed lobes were perfused and ventilated proportionally to the number of segments. The quality of life after partial pulmonary resections had deteriorated only to a small extent compared with the preoperative state. These results were emphasized by the only poor result which (after atelectasis of the anastomosed lung) corresponded functionally to a pneumonectomy. --The two cases of anastomosis of pulmonary artery showed a short survival. Both corresponded to a stage II of tumor classification. Of the other patients (all stage I) three are alive more than five years."}
{"id": "PMID:468563", "title": "[Breast cancer in males].", "content": "Until 1971 13 cases of breast cancer in men were observed in our service. In the world literature it accounts for 1% of all cancer of the breast; in our series we have found 4% of all breast cancer. Men present themselves for treatment later and with more advanced local signs than women. In our series, the greatest number of cases occurs near the seventies in men, which is about twenty years later than in women. The etiology is remained unknown, but Klinefelter's disease seems to be a risk for breast cancer. If there are no metastasis, we prefer in our service the surgical treatment as unique treatment. If possible the operation of Patey is realised, but sometimes we have to do a simple mastectomy only as a safe alternative for poor risk patient. In case of metastatic breast cancer the adjuvant treatment was X-ray therapy, chemotherapy and in some of our cases hormonotherapy. We do not have any experience with suppressive hormonal therapy such as orchiectomy or adrenalectomy. Most of the authors think that prognosis of breast cancer in males is worse than in women; the constant central localization of the tumour may be an explanation for that. The fate of women with central lesions is as poor as in males.", "contents": "[Breast cancer in males]. Until 1971 13 cases of breast cancer in men were observed in our service. In the world literature it accounts for 1% of all cancer of the breast; in our series we have found 4% of all breast cancer. Men present themselves for treatment later and with more advanced local signs than women. In our series, the greatest number of cases occurs near the seventies in men, which is about twenty years later than in women. The etiology is remained unknown, but Klinefelter's disease seems to be a risk for breast cancer. If there are no metastasis, we prefer in our service the surgical treatment as unique treatment. If possible the operation of Patey is realised, but sometimes we have to do a simple mastectomy only as a safe alternative for poor risk patient. In case of metastatic breast cancer the adjuvant treatment was X-ray therapy, chemotherapy and in some of our cases hormonotherapy. We do not have any experience with suppressive hormonal therapy such as orchiectomy or adrenalectomy. Most of the authors think that prognosis of breast cancer in males is worse than in women; the constant central localization of the tumour may be an explanation for that. The fate of women with central lesions is as poor as in males."}
{"id": "PMID:468564", "title": "[Vaccination against staphylococcal infections in animal experiments].", "content": "Based on favour and clinical observations we have undertaken to observe the effect of vaccination against staphylococcic infection in the mouse. A standard amount of staphylococcus was used together with a metal implant. After two weeks in the control group always an infection could be observed, and germs could be cultivated. In the groups with staphypan and in the groups with vaccinated specific vaccinia a less strong infection was observed, while a vaccinia made from salmonella typhimurium and diphtheria-toxoid showed the strongest effect. The use of unspecific vaccinia deserves further attention in relation to operations which can be made after vaccination of the patient.", "contents": "[Vaccination against staphylococcal infections in animal experiments]. Based on favour and clinical observations we have undertaken to observe the effect of vaccination against staphylococcic infection in the mouse. A standard amount of staphylococcus was used together with a metal implant. After two weeks in the control group always an infection could be observed, and germs could be cultivated. In the groups with staphypan and in the groups with vaccinated specific vaccinia a less strong infection was observed, while a vaccinia made from salmonella typhimurium and diphtheria-toxoid showed the strongest effect. The use of unspecific vaccinia deserves further attention in relation to operations which can be made after vaccination of the patient."}
{"id": "PMID:468565", "title": "[Inclined plate screws - in vitro measurement of the stability of transverse osteotomy of tibia].", "content": "A compression plate which is exactly contoured to the bone surface produces asymmetric compression: only part of the cortex near the plate is compressed. Therefore a compression plate is not meant to be used selfstanding, it will rather be combined with additional means. Two of these have been studied: the increased stability provided by the use of lagging, inclined plate screws was compared to the one provided by the spring effect of a plate prebent for central elevation. 7-hole dynamic compression plates (DCP) and 4.5 mm cortex screws were used to stabilize transverse osteotomies in human tibiae in vitro. Flexural and torsional testing revealed that the use of an inclined lag screw or a prebent plate increases stability compared to the one achieved with an exactly contoured plate alone. Preliminary tests suggest, that a combination of plate lag-screw and prebending offers stability up to high loads.", "contents": "[Inclined plate screws - in vitro measurement of the stability of transverse osteotomy of tibia]. A compression plate which is exactly contoured to the bone surface produces asymmetric compression: only part of the cortex near the plate is compressed. Therefore a compression plate is not meant to be used selfstanding, it will rather be combined with additional means. Two of these have been studied: the increased stability provided by the use of lagging, inclined plate screws was compared to the one provided by the spring effect of a plate prebent for central elevation. 7-hole dynamic compression plates (DCP) and 4.5 mm cortex screws were used to stabilize transverse osteotomies in human tibiae in vitro. Flexural and torsional testing revealed that the use of an inclined lag screw or a prebent plate increases stability compared to the one achieved with an exactly contoured plate alone. Preliminary tests suggest, that a combination of plate lag-screw and prebending offers stability up to high loads."}
{"id": "PMID:468566", "title": "[Importance of the friction between plate and bone in the anchoring of plates for osteosynthesis. Determination of the coefficient of metal-bone friction in animal in vivo].", "content": "In mechanical devices in general the friction plays an important role in force transmission. To find out about the magnitude of frictional forces between internal fixation plate and bone in vivo, simultaneous strain recording in plated bone and instrumented plate were performed. After 3 weeks of implantation the torque applied to a single plate-screw was set to definite levels. Thus it could be determined at what level of screw compression and at what tangential load of the related surface bone-plate, gliding occurred. The coefficient of friction was found to be 0.2 +/- 0.09 (x +/- Sx). It can be concluded that in vivo friction produced at the plate-bone contact surface is able to guarantee stability of fixation.", "contents": "[Importance of the friction between plate and bone in the anchoring of plates for osteosynthesis. Determination of the coefficient of metal-bone friction in animal in vivo]. In mechanical devices in general the friction plays an important role in force transmission. To find out about the magnitude of frictional forces between internal fixation plate and bone in vivo, simultaneous strain recording in plated bone and instrumented plate were performed. After 3 weeks of implantation the torque applied to a single plate-screw was set to definite levels. Thus it could be determined at what level of screw compression and at what tangential load of the related surface bone-plate, gliding occurred. The coefficient of friction was found to be 0.2 +/- 0.09 (x +/- Sx). It can be concluded that in vivo friction produced at the plate-bone contact surface is able to guarantee stability of fixation."}
{"id": "PMID:468567", "title": "[A method of quantitative determination of tissue tolerance in decreased relative movement between implant and tissue].", "content": "A method of testing in vivo tissue tolerance to different implant materials is presented. It aims at reduction of variance due to mechanical irritation at the tissue-implant interface through the use of cylindrical implants with grooves. The special type of histological preparation which is necessary and problems encountered when using these techniques are described. The numerical evaluation of round cells and foreign body giant cells seems to give uniform results with low variance, which could be of interest for standard testing of material.", "contents": "[A method of quantitative determination of tissue tolerance in decreased relative movement between implant and tissue]. A method of testing in vivo tissue tolerance to different implant materials is presented. It aims at reduction of variance due to mechanical irritation at the tissue-implant interface through the use of cylindrical implants with grooves. The special type of histological preparation which is necessary and problems encountered when using these techniques are described. The numerical evaluation of round cells and foreign body giant cells seems to give uniform results with low variance, which could be of interest for standard testing of material."}
{"id": "PMID:468569", "title": "[Regional gastric blood flow in hypovolemic shock in rats].", "content": "The effect of hypovolemia on the mucosal blood flow of gastric corpus and antrum were tested with the microspheres method 30 and 60 min after induction of shock in the rat. The results demonstrate a marked reduction after 30 min, with return to control values after 60 min, without any significant differences between corpus and antrum. It is concluded that the differences in energy metabolism between these two regions of gastric mucosa, cannot be attributed to different degrees of ischemia.", "contents": "[Regional gastric blood flow in hypovolemic shock in rats]. The effect of hypovolemia on the mucosal blood flow of gastric corpus and antrum were tested with the microspheres method 30 and 60 min after induction of shock in the rat. The results demonstrate a marked reduction after 30 min, with return to control values after 60 min, without any significant differences between corpus and antrum. It is concluded that the differences in energy metabolism between these two regions of gastric mucosa, cannot be attributed to different degrees of ischemia."}
{"id": "PMID:468570", "title": "[Acute pancreatitis in animal experiments - a new method].", "content": "Acute pancreatitis was induced in 30 minipigs (G\u00f6ttingen) by implantation of a T-drain into the Ductus Wirsungianus. The distal part of the T-drain, which led into the duodenum was stenosed to 1/3 of the original diameter. The inflammation was caused by stenosis of the Ductus Wirsungianus additionally by application of contrast medium. The course of the inflammation was demonstrated by biochemical, roentgenological and histological examinations.", "contents": "[Acute pancreatitis in animal experiments - a new method]. Acute pancreatitis was induced in 30 minipigs (G\u00f6ttingen) by implantation of a T-drain into the Ductus Wirsungianus. The distal part of the T-drain, which led into the duodenum was stenosed to 1/3 of the original diameter. The inflammation was caused by stenosis of the Ductus Wirsungianus additionally by application of contrast medium. The course of the inflammation was demonstrated by biochemical, roentgenological and histological examinations."}
{"id": "PMID:468571", "title": "[In vitro study of isolated chondrocytes concerning the prognosis of transplanted cartilage].", "content": "In order to find out reliable criteria to conclude on the prognosis of transplanted cartilage the quality of isolated rabbit chondrocytes was assayed in vitro. Using a semisolid medium a culture system is introduced, which is able to detect colony forming units of chondrocytes. Furthermore their proliferative and differentiating capacity was investigated. Colony formation in vitro follows a strict dose response relationship. An inverse relationship was observed between the age of the donor and the colony incidence in vitro. Cells from neonatal cartilage could be induced to grow as fibroblastic colonies in the presence of conditioned medium from fibroblastic monolayer cultures. No effect, however, could be observed with chondrocyte derived conditioned medium.", "contents": "[In vitro study of isolated chondrocytes concerning the prognosis of transplanted cartilage]. In order to find out reliable criteria to conclude on the prognosis of transplanted cartilage the quality of isolated rabbit chondrocytes was assayed in vitro. Using a semisolid medium a culture system is introduced, which is able to detect colony forming units of chondrocytes. Furthermore their proliferative and differentiating capacity was investigated. Colony formation in vitro follows a strict dose response relationship. An inverse relationship was observed between the age of the donor and the colony incidence in vitro. Cells from neonatal cartilage could be induced to grow as fibroblastic colonies in the presence of conditioned medium from fibroblastic monolayer cultures. No effect, however, could be observed with chondrocyte derived conditioned medium."}
{"id": "PMID:468572", "title": "[Morphological and biochemical examinations of different suture materials in colon anastomosis in the rat].", "content": "Histological, mechanical and biochemical examinations in the one layer colon anastomosis of the rat have shown that the synthetic resorbable suture material as Dexon and Vicryl are as good or even better than silk or catgut chromic. The most favourable histological reaction is observed with Dexon. Collagenolytic activity and collagen content are not influenced by the suture material during the first 14 postoperative days.", "contents": "[Morphological and biochemical examinations of different suture materials in colon anastomosis in the rat]. Histological, mechanical and biochemical examinations in the one layer colon anastomosis of the rat have shown that the synthetic resorbable suture material as Dexon and Vicryl are as good or even better than silk or catgut chromic. The most favourable histological reaction is observed with Dexon. Collagenolytic activity and collagen content are not influenced by the suture material during the first 14 postoperative days."}
{"id": "PMID:468573", "title": "[Autoimplantation of splenic tissue into the peritoneal cavity of irradiated rats].", "content": "A simple technique is described for the autoimplantation of splenic tissue in the peritoneal cavity of rats. The effect of splenic implants on irradiated splenectomised rats was investigated, by following the evolution of the implants both microscopically and macroscopically. The effect on haemopoiesis was also observed. The dose of irradiation given was sublethal, do not think it necessary to put in, and non lethal.", "contents": "[Autoimplantation of splenic tissue into the peritoneal cavity of irradiated rats]. A simple technique is described for the autoimplantation of splenic tissue in the peritoneal cavity of rats. The effect of splenic implants on irradiated splenectomised rats was investigated, by following the evolution of the implants both microscopically and macroscopically. The effect on haemopoiesis was also observed. The dose of irradiation given was sublethal, do not think it necessary to put in, and non lethal."}
{"id": "PMID:468574", "title": "[Morphological liver changes after portacaval and splenocaval anastomosis in rats].", "content": "Body weight, liver weight and liver histology in rats 3 weeks after portacaval and distal splenocaval anastomosis were analyzed. Rats with portacaval shunt showed a reduction of body and liver weight and histological signs of liver atrophy. There was no difference between the results in rats with splenocaval shunt and controls.", "contents": "[Morphological liver changes after portacaval and splenocaval anastomosis in rats]. Body weight, liver weight and liver histology in rats 3 weeks after portacaval and distal splenocaval anastomosis were analyzed. Rats with portacaval shunt showed a reduction of body and liver weight and histological signs of liver atrophy. There was no difference between the results in rats with splenocaval shunt and controls."}
{"id": "PMID:468575", "title": "[Compression of the celiac trunk by the median arcuate ligament].", "content": "A relatively large series of 20 operated patients, aged 28 to 66 years, with median arcuate ligament syndrome is presented. There has been a change in the pattern of presentation of this condition in recent years. Early diagnosis can now be made with greater frequency than previously. The origin and the symptoms due to the intermittent, chronic abdominal ischaemia are discussed. The preliminary results suggest that surgical decompression is effective in inducing favourable changes in 60% of patients.", "contents": "[Compression of the celiac trunk by the median arcuate ligament]. A relatively large series of 20 operated patients, aged 28 to 66 years, with median arcuate ligament syndrome is presented. There has been a change in the pattern of presentation of this condition in recent years. Early diagnosis can now be made with greater frequency than previously. The origin and the symptoms due to the intermittent, chronic abdominal ischaemia are discussed. The preliminary results suggest that surgical decompression is effective in inducing favourable changes in 60% of patients."}
{"id": "PMID:468576", "title": "[2 years experience with the Octopus universal retractor holder].", "content": "On 978 operations of all fields of surgery, the new automatic retractor holder has been successfully used. The holder arms are provided with three joints and can be secured in any desired position to keep the edges of the incision and organs in the necessary situation for operating. All joints are locked by operating the central pivot. The advantages of this new automatic retractor holder was described.", "contents": "[2 years experience with the Octopus universal retractor holder]. On 978 operations of all fields of surgery, the new automatic retractor holder has been successfully used. The holder arms are provided with three joints and can be secured in any desired position to keep the edges of the incision and organs in the necessary situation for operating. All joints are locked by operating the central pivot. The advantages of this new automatic retractor holder was described."}
{"id": "PMID:468577", "title": "[Diagnostic arthroscopy and arthroscopic surgery: experiences with 500 knee arthroscopies].", "content": "A diagnosis by clinical examination and arthrography was not possible in 160 out of 500 arthroscopically examined patients, most of them with post-traumatic knee disorders. In 157 cases the clinical diagnosis was wrong, and in another 58 cases incomplete. Only in 89 patients (18%) arthroscopy proved the clinical diagnosis to be correct. At arthroscopy, a meniscal injury was found in 156 patients (medial meniscus 57, lateral meniscus 64, both menisci 8). With regard to the menisci a previous arthrography was found correct only in 103 out of 213 cases, i.e. in 48%. Lesions of the articular cartilage were present in 210 patients, although they were expected clinically in only one third of these cases. Normal intraarticular structures were found in 95 examinations. The arthroscopic examination was insufficient three times because of a protruding fat pad, and wrong in 2 patients in whom an arthroscopically diagnosed meniscal tear could not be found at arthrotomy. The morbidity of arthroscopy is small. Only complications: A local allergic reaction because of a wound spray in four cases, bronchial asthma following general anesthesia in two patients. No infection occurred. Several therapeutic procedures may be carried out through the arthroscope. So loose bodies were removed from the joint in 39 and partial meniscectomy performed in 13 patients, all of them being treated as out-patients.", "contents": "[Diagnostic arthroscopy and arthroscopic surgery: experiences with 500 knee arthroscopies]. A diagnosis by clinical examination and arthrography was not possible in 160 out of 500 arthroscopically examined patients, most of them with post-traumatic knee disorders. In 157 cases the clinical diagnosis was wrong, and in another 58 cases incomplete. Only in 89 patients (18%) arthroscopy proved the clinical diagnosis to be correct. At arthroscopy, a meniscal injury was found in 156 patients (medial meniscus 57, lateral meniscus 64, both menisci 8). With regard to the menisci a previous arthrography was found correct only in 103 out of 213 cases, i.e. in 48%. Lesions of the articular cartilage were present in 210 patients, although they were expected clinically in only one third of these cases. Normal intraarticular structures were found in 95 examinations. The arthroscopic examination was insufficient three times because of a protruding fat pad, and wrong in 2 patients in whom an arthroscopically diagnosed meniscal tear could not be found at arthrotomy. The morbidity of arthroscopy is small. Only complications: A local allergic reaction because of a wound spray in four cases, bronchial asthma following general anesthesia in two patients. No infection occurred. Several therapeutic procedures may be carried out through the arthroscope. So loose bodies were removed from the joint in 39 and partial meniscectomy performed in 13 patients, all of them being treated as out-patients."}
{"id": "PMID:468578", "title": "[Surgical intervention in traumatic orbital syndromes].", "content": "Craniofacial traumata may be accompanied by retroorbital fractures with severe ophthalmologic complications. Each of the clinical syndromes is characterized by a combination of ophthalmoplegia, paralyzed pupillary function and visual disorder. These deficiencies are caused by injury of muscles, nerves and vessels and compressive retroorbital hematoma. In the case of such damage, the poor prognosis can be improved by surgical intervention consisting in the evacuation of the retroorbital hematoma.", "contents": "[Surgical intervention in traumatic orbital syndromes]. Craniofacial traumata may be accompanied by retroorbital fractures with severe ophthalmologic complications. Each of the clinical syndromes is characterized by a combination of ophthalmoplegia, paralyzed pupillary function and visual disorder. These deficiencies are caused by injury of muscles, nerves and vessels and compressive retroorbital hematoma. In the case of such damage, the poor prognosis can be improved by surgical intervention consisting in the evacuation of the retroorbital hematoma."}
{"id": "PMID:468579", "title": "[Principles of treating facial fractures].", "content": "The therapy of facial fractures is aimed at an early definitive treatment. Exact repositioning of the fragments is first achieved by setting the right occlusion. Intermaxillary fixation is then performed by interdental arch bars reenforced with acrylic material and intermaxillary rubber bands or wire. The middle third of the face is mainly subjected to static forces. Fractures in this area are therefore set and fixated with interosseous wire ligatures. Craneofacial suspension is carried out by means of a craneofixateur extern. Fractures of the mandible are treated differently. In order to counteract the strong dynamic forces internal fixation is necessary using compression plates in combination with tension bands. Another modality of treatment by a specially designed plate with excentric gliding holes renders the tension bands superfluous since it is capable of applying compression forces also at the dental area. In fractures with osseous defects long plates are used which stabilize the mandibular arch. If the articular prominence is missing these plates may be applied with a capitular end in order to reconstruct the articulation.", "contents": "[Principles of treating facial fractures]. The therapy of facial fractures is aimed at an early definitive treatment. Exact repositioning of the fragments is first achieved by setting the right occlusion. Intermaxillary fixation is then performed by interdental arch bars reenforced with acrylic material and intermaxillary rubber bands or wire. The middle third of the face is mainly subjected to static forces. Fractures in this area are therefore set and fixated with interosseous wire ligatures. Craneofacial suspension is carried out by means of a craneofixateur extern. Fractures of the mandible are treated differently. In order to counteract the strong dynamic forces internal fixation is necessary using compression plates in combination with tension bands. Another modality of treatment by a specially designed plate with excentric gliding holes renders the tension bands superfluous since it is capable of applying compression forces also at the dental area. In fractures with osseous defects long plates are used which stabilize the mandibular arch. If the articular prominence is missing these plates may be applied with a capitular end in order to reconstruct the articulation."}
{"id": "PMID:468580", "title": "[Prognosis of iatrogenic and accidental vascular trauma].", "content": "The prognostic of vascular trauma does not only depend on the associated lesions and time before treatment. Without obvious symptoms, serious vascular lesions can be overlooked. A preexisting arteriosclerosis explains the different outcome in geriatric patients. 84 cases of vascular trauma were observed between 1969 and 1977. 33 of these cases were iatrogenic. The patients involved are examined clinically and undergo ultrasonic velocity detection before and after effort (test of Strandness). The outcome showed 5 deaths, 7 amputations and 8 vascular sequela. Only 2 deaths and 3 amputations are directly related to the vascular trauma.", "contents": "[Prognosis of iatrogenic and accidental vascular trauma]. The prognostic of vascular trauma does not only depend on the associated lesions and time before treatment. Without obvious symptoms, serious vascular lesions can be overlooked. A preexisting arteriosclerosis explains the different outcome in geriatric patients. 84 cases of vascular trauma were observed between 1969 and 1977. 33 of these cases were iatrogenic. The patients involved are examined clinically and undergo ultrasonic velocity detection before and after effort (test of Strandness). The outcome showed 5 deaths, 7 amputations and 8 vascular sequela. Only 2 deaths and 3 amputations are directly related to the vascular trauma."}
{"id": "PMID:468581", "title": "[Determination of the conditions of extra-anatomical arterial cross-over-shunts].", "content": "Extra anatomical cross-over-shunts are feasible even when the lending extremity is itself afflicted by obliterative arterial disease provided that 1. global irrigation of the lending extremity exceeds perfusion of the borrowing extremity, 2. pulse-volume-recording (PVR) corresponds to categories 1--3 as defined by Raines, 3. ankle pressure of the lending extremity is not less than 70 mm of mercury or 4. ankle pressure of the leading extremity corresponds to 60% or more than the systemic blood pressure.", "contents": "[Determination of the conditions of extra-anatomical arterial cross-over-shunts]. Extra anatomical cross-over-shunts are feasible even when the lending extremity is itself afflicted by obliterative arterial disease provided that 1. global irrigation of the lending extremity exceeds perfusion of the borrowing extremity, 2. pulse-volume-recording (PVR) corresponds to categories 1--3 as defined by Raines, 3. ankle pressure of the lending extremity is not less than 70 mm of mercury or 4. ankle pressure of the leading extremity corresponds to 60% or more than the systemic blood pressure."}
{"id": "PMID:468582", "title": "[Infection after vascular surgery].", "content": "Infection after vascular surgery is a feared complication whose mortality rate remains high, inspite of an aggressive surgical approach, actually admitted by most of the authors. From 1970 to 1977 we have observed 6 infections after 1049 arterial operations: 5 out of 228 synthetic by-passes and 1 out of 296 venous by-passes. The global infection rate is 0.5%. This complication occurred in 2.1% of the cases after synthetic by-passes. Two out of these 6 infected patients had to be amputated and they died. We think that the general accepted treatment remains valuable in most of the cases, but that a more simple and less aggressive surgical approach can be of great interest in particular situations.", "contents": "[Infection after vascular surgery]. Infection after vascular surgery is a feared complication whose mortality rate remains high, inspite of an aggressive surgical approach, actually admitted by most of the authors. From 1970 to 1977 we have observed 6 infections after 1049 arterial operations: 5 out of 228 synthetic by-passes and 1 out of 296 venous by-passes. The global infection rate is 0.5%. This complication occurred in 2.1% of the cases after synthetic by-passes. Two out of these 6 infected patients had to be amputated and they died. We think that the general accepted treatment remains valuable in most of the cases, but that a more simple and less aggressive surgical approach can be of great interest in particular situations."}
{"id": "PMID:468584", "title": "[Deep venous thrombosis of the lower limb: experiences of thrombectomy within and outside the iliacofemoral segment].", "content": "A series of 23 venous thrombectomies of the lower limb is presented. In 19 instances indirect thrombectomy of the iliacofemoral segment was performed. 1 direct thrombectomy of the v. cava inferior led to full lumen restoration. Direct thrombectomy of the popliteal vein was attempted in 3 cases, resulting in phlebographic and clinical normalization in 2 instances. In no instance did pulmonary embolism occur during or after venous thrombectomy.", "contents": "[Deep venous thrombosis of the lower limb: experiences of thrombectomy within and outside the iliacofemoral segment]. A series of 23 venous thrombectomies of the lower limb is presented. In 19 instances indirect thrombectomy of the iliacofemoral segment was performed. 1 direct thrombectomy of the v. cava inferior led to full lumen restoration. Direct thrombectomy of the popliteal vein was attempted in 3 cases, resulting in phlebographic and clinical normalization in 2 instances. In no instance did pulmonary embolism occur during or after venous thrombectomy."}
{"id": "PMID:468585", "title": "[Registration of fibular movements in vivo in relation to the tibia at the level of the ankle joint].", "content": "Fibular movements in relation to the tibia are known on extension and dorsiflexion of the ankle-joint and have been proved by experiences on cadaveric preparates using outstanding points of relation. To registrate fibular movements in vivo a special trigonometric system has been developed permitting calculation of definite movement in frontal and sagittal plane as well as a rotation around axis. Twelve healthy volunteers were examined in this way during extension and dorsiflexion of the ankle, with and without weight-bearing. The results showed that we should not consider isolated fibular movement but analyse simultaneous complexity of movements in a graphic comparison. Under these conditions we observe a very high significance of fibular movements depending each of other. The emphasis can be designed in a field covering all the points reached by fibular movement in frontal and sagittal plane of the twelve healthy volunteers during movement of the ankle; it shows that the fibula functions like a guide-stick for ankle movement. Reflecting on the designed fields of healthy persons, some patients with posttraumatic pathological alterations of the ankle-joint have been analysed.", "contents": "[Registration of fibular movements in vivo in relation to the tibia at the level of the ankle joint]. Fibular movements in relation to the tibia are known on extension and dorsiflexion of the ankle-joint and have been proved by experiences on cadaveric preparates using outstanding points of relation. To registrate fibular movements in vivo a special trigonometric system has been developed permitting calculation of definite movement in frontal and sagittal plane as well as a rotation around axis. Twelve healthy volunteers were examined in this way during extension and dorsiflexion of the ankle, with and without weight-bearing. The results showed that we should not consider isolated fibular movement but analyse simultaneous complexity of movements in a graphic comparison. Under these conditions we observe a very high significance of fibular movements depending each of other. The emphasis can be designed in a field covering all the points reached by fibular movement in frontal and sagittal plane of the twelve healthy volunteers during movement of the ankle; it shows that the fibula functions like a guide-stick for ankle movement. Reflecting on the designed fields of healthy persons, some patients with posttraumatic pathological alterations of the ankle-joint have been analysed."}
{"id": "PMID:468587", "title": "[Early scanning electron microscopy changes of the surface of the leaflets of orthotopic transplanted glyceraldehyd-conditioned conus-pulmonalis xenografts. An experimental study in dogs].", "content": "The surface of the leaflets of orthotopic transplanted glutaraldehyd-conditioned conus-pulmonalis xenografts show when analysed by a scanning microscope the following constant changes. Within seconds the surface of the leaflets is covered by a grainy layer of proteins followed by fibrin threads. Cellular elements of the blood--like platelets, white blood cells, macrophages and microthrombi--are incorporated in the fibrin network. This leads, after several weeks to a fibrous layer especially over the stiff parts of the valve. No endothelium was seen even after 6 months.", "contents": "[Early scanning electron microscopy changes of the surface of the leaflets of orthotopic transplanted glyceraldehyd-conditioned conus-pulmonalis xenografts. An experimental study in dogs]. The surface of the leaflets of orthotopic transplanted glutaraldehyd-conditioned conus-pulmonalis xenografts show when analysed by a scanning microscope the following constant changes. Within seconds the surface of the leaflets is covered by a grainy layer of proteins followed by fibrin threads. Cellular elements of the blood--like platelets, white blood cells, macrophages and microthrombi--are incorporated in the fibrin network. This leads, after several weeks to a fibrous layer especially over the stiff parts of the valve. No endothelium was seen even after 6 months."}
{"id": "PMID:468588", "title": "[Scanning electron microscopy study of autologous fascia lata and bovine xenograft as arterial prosthesis. An experimental study].", "content": "The present communication deals with the decomposition of thrombi and the formation of an endothelium in two different vascular prothesis. Devitalized bovine arteries which were enzymatically reduced to a collagen reticulum, and tubes made of autologous fascia lata were implanted into the arterial bloodstream of dogs. The implants were left in place for a time ranging between 30 min and four months. They were examined by scanning electronmicroscope with the cortical point method. This enabled us to reach the following conclusions: unprepared autologous fascia lata implanted into the arterial bloodstream has much more thrombogenous qualities than solcograft. The decomposition of thrombi and the formation of an endothelium is much faster accomplished in fascia lata than in the devitalized bovine artery. It is not possible to distinguish solcograft and fascia lata by the formation of endothelium only.", "contents": "[Scanning electron microscopy study of autologous fascia lata and bovine xenograft as arterial prosthesis. An experimental study]. The present communication deals with the decomposition of thrombi and the formation of an endothelium in two different vascular prothesis. Devitalized bovine arteries which were enzymatically reduced to a collagen reticulum, and tubes made of autologous fascia lata were implanted into the arterial bloodstream of dogs. The implants were left in place for a time ranging between 30 min and four months. They were examined by scanning electronmicroscope with the cortical point method. This enabled us to reach the following conclusions: unprepared autologous fascia lata implanted into the arterial bloodstream has much more thrombogenous qualities than solcograft. The decomposition of thrombi and the formation of an endothelium is much faster accomplished in fascia lata than in the devitalized bovine artery. It is not possible to distinguish solcograft and fascia lata by the formation of endothelium only."}
{"id": "PMID:468589", "title": "Localization of lactate dehydrogenase isozymes in human muscle tissues by the mixed aggregation immunocytochemical technique.", "content": "Lactate dehydrogenase (LDH) isozyme composition and localization was determined in sections of skeletal, heart and smooth muscle by the mixed aggregation immunocytochemical method using first antibody directed against purified human LDH-A4 (M4) or LDH-B4 (H4) followed by the enzymes LDH-A4 and LDH-B4, respectively. An even distribution of the two monomers in all fibres was seen with heart muscle and smooth muscle. Heart muscle had a low concentration of A-monomers and a high concentration of B-monomers, whereas the smooth muscle had equal concentrations of the two monomers. In contrast, skeletal muscle from m. quadriceps femoris was found to be composed of two muscle fibre types, one containing mainly A-, the other mainly B-monomers. On the basis of succinate dehydrogenase activity it was shown that the red (type 1) fibres contain mainly B-monomers and the white (type 2) fibres mainly A-monomers of LDH.", "contents": "Localization of lactate dehydrogenase isozymes in human muscle tissues by the mixed aggregation immunocytochemical technique. Lactate dehydrogenase (LDH) isozyme composition and localization was determined in sections of skeletal, heart and smooth muscle by the mixed aggregation immunocytochemical method using first antibody directed against purified human LDH-A4 (M4) or LDH-B4 (H4) followed by the enzymes LDH-A4 and LDH-B4, respectively. An even distribution of the two monomers in all fibres was seen with heart muscle and smooth muscle. Heart muscle had a low concentration of A-monomers and a high concentration of B-monomers, whereas the smooth muscle had equal concentrations of the two monomers. In contrast, skeletal muscle from m. quadriceps femoris was found to be composed of two muscle fibre types, one containing mainly A-, the other mainly B-monomers. On the basis of succinate dehydrogenase activity it was shown that the red (type 1) fibres contain mainly B-monomers and the white (type 2) fibres mainly A-monomers of LDH."}
{"id": "PMID:468590", "title": "A gel-sandwich technique for the qualitative and quantitative determination of dehydrogenases in the enzyme histochemistry. I. Development of the new methods on the example of LDH (E.C. 1.1.1.27).", "content": "A gel-sandwich technique for the histochemical demonstration of dehydrogenase is introduced with LDH set up as an example. Especially suitable, of the gels examined, for this technique is 1.5% W/V agar-agar low gel strength. In it several reaction ingredients for the histochemical reaction are dissolved. Considering LDH the following gel composition showed good results: 1.5% W/V agar-agar low gel strength, 5 mM TNBT in 150 microliter DMF, 120 mM L-lactate, 3--5 mM NAD+, 10 mM amytal, 22,4--32 X 10(-5) M Meldola Blue, 160 mM soldium phosphate buffer pH 7.6 (total solution of 1 ml). After the solidification of the gel, gel-bars were frozen with CO2-snow. The 40--80 micrometer thick gel slices were gained in the cryostat. Of the three different arrangement possibilities of the gel slices and the tissue-sections a sandwich arrangement (cover-gel slice--tissue section--ground-gel slice) produced the best results. The enzyme reaction is started by thawing of the gel slices (together with the tissue sections) and by putting them between the hotplate and the evaporator-head-piece, especially developed for this technique. The gel slices also remain in combination with the tissue sections after the reaction. The influence of the gel in combination with the electron carrier Meldola Blue on the spontaneous reduction rates of ditetrazolium salts in day light, were examined as well as the diffusion rates of TNBT and NADH out of gel slices and the influence of DMF and DMSO on the LDH activity. This technique prevents both, the loss of enzymes and the loss of reduction equivalents. There are given presuppositions for qualitative and quantitative histochemical investigations as well. The advantages of the new gel technique are discussed.", "contents": "A gel-sandwich technique for the qualitative and quantitative determination of dehydrogenases in the enzyme histochemistry. I. Development of the new methods on the example of LDH (E.C. 1.1.1.27). A gel-sandwich technique for the histochemical demonstration of dehydrogenase is introduced with LDH set up as an example. Especially suitable, of the gels examined, for this technique is 1.5% W/V agar-agar low gel strength. In it several reaction ingredients for the histochemical reaction are dissolved. Considering LDH the following gel composition showed good results: 1.5% W/V agar-agar low gel strength, 5 mM TNBT in 150 microliter DMF, 120 mM L-lactate, 3--5 mM NAD+, 10 mM amytal, 22,4--32 X 10(-5) M Meldola Blue, 160 mM soldium phosphate buffer pH 7.6 (total solution of 1 ml). After the solidification of the gel, gel-bars were frozen with CO2-snow. The 40--80 micrometer thick gel slices were gained in the cryostat. Of the three different arrangement possibilities of the gel slices and the tissue-sections a sandwich arrangement (cover-gel slice--tissue section--ground-gel slice) produced the best results. The enzyme reaction is started by thawing of the gel slices (together with the tissue sections) and by putting them between the hotplate and the evaporator-head-piece, especially developed for this technique. The gel slices also remain in combination with the tissue sections after the reaction. The influence of the gel in combination with the electron carrier Meldola Blue on the spontaneous reduction rates of ditetrazolium salts in day light, were examined as well as the diffusion rates of TNBT and NADH out of gel slices and the influence of DMF and DMSO on the LDH activity. This technique prevents both, the loss of enzymes and the loss of reduction equivalents. There are given presuppositions for qualitative and quantitative histochemical investigations as well. The advantages of the new gel technique are discussed."}
{"id": "PMID:468591", "title": "Cytochemical reaction for cationic proteins as a marker of primary granules during development in chick heterophilis.", "content": "The ammoniacal silver reaction (ASR) for cationic proteins was used as a cytochemical marker for the primary or A granules in the cytoplasm of developing heterophils of chick bone marrow. The presence of the electron-dense particulate reaction product of silver, which is localized in the fully formed rod-shaped A granules, provides a marker by which the A granules could be distinguished from the B granules of similar size and by which the formation and maturation of both granule types could be followed through the developmental stages. Progressive developmental stages were ascertained on the basis of decreasing cell size, increasing condensation and margination of the chromatin, and the number and morphology of the granules; the stages were divided into promyelocyte, myelocyte, metamyelocyte and heterophil. During the promyelocyte stage, the first appearance of the electron-dense, membrane-bound, spherical granules (0.3--1.0 micrometer in diameter) is observed in the vicinity of an extensive Golgi complex. They occur in a cytoplasm containing rough-surfaced endoplasmic reticulum, ribosomal clusters, centrioles, mitochondria, microtubules, as well as the membranes, saccules, vesicles and vacuoles of the Golgi complex. These granules are considered as primary but their presence as the only granule type appears very brief. The ASR reaction product is first detected on the surface of these primary granules in late promyelocytes or myelocytes. The secondary or B granule, devoid of reaction for cationic protein at all stages, appears as a condensing vacuole in promyelocytes, but after some A granules are already present. The vacuole contents condense to form the B granules which are 0.1--0.6 micrometer in diameter, often oval-shaped, and contain a loose filamentous material surrounded by a membrane. Tertiary C granules or lysosomes appear during the myelocyte stage as dense core vesicles (0.1--0.2 micrometer in diameter) negative for cationic protein.", "contents": "Cytochemical reaction for cationic proteins as a marker of primary granules during development in chick heterophilis. The ammoniacal silver reaction (ASR) for cationic proteins was used as a cytochemical marker for the primary or A granules in the cytoplasm of developing heterophils of chick bone marrow. The presence of the electron-dense particulate reaction product of silver, which is localized in the fully formed rod-shaped A granules, provides a marker by which the A granules could be distinguished from the B granules of similar size and by which the formation and maturation of both granule types could be followed through the developmental stages. Progressive developmental stages were ascertained on the basis of decreasing cell size, increasing condensation and margination of the chromatin, and the number and morphology of the granules; the stages were divided into promyelocyte, myelocyte, metamyelocyte and heterophil. During the promyelocyte stage, the first appearance of the electron-dense, membrane-bound, spherical granules (0.3--1.0 micrometer in diameter) is observed in the vicinity of an extensive Golgi complex. They occur in a cytoplasm containing rough-surfaced endoplasmic reticulum, ribosomal clusters, centrioles, mitochondria, microtubules, as well as the membranes, saccules, vesicles and vacuoles of the Golgi complex. These granules are considered as primary but their presence as the only granule type appears very brief. The ASR reaction product is first detected on the surface of these primary granules in late promyelocytes or myelocytes. The secondary or B granule, devoid of reaction for cationic protein at all stages, appears as a condensing vacuole in promyelocytes, but after some A granules are already present. The vacuole contents condense to form the B granules which are 0.1--0.6 micrometer in diameter, often oval-shaped, and contain a loose filamentous material surrounded by a membrane. Tertiary C granules or lysosomes appear during the myelocyte stage as dense core vesicles (0.1--0.2 micrometer in diameter) negative for cationic protein."}
{"id": "PMID:468592", "title": "Histochemical demonstration of mouse submandibular esterproteases with a new chromogenic substrate.", "content": "A new method for the histochemical demonstration of mouse submandibular esterproteases has been developed. The substrate is N-acetyl-L-methionine alpha-naphthyl ester. The main enzyme reaction was found in the apical region of the secretory tubules with a marked sex difference as expected. First attempts were made to differentiate histochemically between the various esterproteolytic enzymes.", "contents": "Histochemical demonstration of mouse submandibular esterproteases with a new chromogenic substrate. A new method for the histochemical demonstration of mouse submandibular esterproteases has been developed. The substrate is N-acetyl-L-methionine alpha-naphthyl ester. The main enzyme reaction was found in the apical region of the secretory tubules with a marked sex difference as expected. First attempts were made to differentiate histochemically between the various esterproteolytic enzymes."}
{"id": "PMID:468599", "title": "Influence of expiratory flow rate on \"closing volume\" measurement.", "content": "The influence of expiratory flow rate (VE) on the onset of phase IV was studied in 15 normal subjects. VE was controlled voluntarily and varied between 0.2 and 2.5 l/s. All subjects showed significantly higher values for CV/VC, % at expiratory flow rate of 1.0 and 1.5 l/s, compared to those estimated at 0.2 l/s. The correlation between CV and volume of flow limitation (VFL) was also studied. For most of the subjects a considerable disagreement between the two values at very low VE was found. At higher flows, however, CV and VFL agreed well. Our results indicate that CV measurement is markedly influenced by VE in the range 0.2--1.5 l/s. This finding gives further support to the hypothesis that CV is determined in part by dynamic flow-limiting properties of the lung as well as by true airway closure.", "contents": "Influence of expiratory flow rate on \"closing volume\" measurement. The influence of expiratory flow rate (VE) on the onset of phase IV was studied in 15 normal subjects. VE was controlled voluntarily and varied between 0.2 and 2.5 l/s. All subjects showed significantly higher values for CV/VC, % at expiratory flow rate of 1.0 and 1.5 l/s, compared to those estimated at 0.2 l/s. The correlation between CV and volume of flow limitation (VFL) was also studied. For most of the subjects a considerable disagreement between the two values at very low VE was found. At higher flows, however, CV and VFL agreed well. Our results indicate that CV measurement is markedly influenced by VE in the range 0.2--1.5 l/s. This finding gives further support to the hypothesis that CV is determined in part by dynamic flow-limiting properties of the lung as well as by true airway closure."}
{"id": "PMID:468600", "title": "Tonometer for calibration and evaluation of oxygen microelectrodes.", "content": "A temperature-controlled tonometer for the calibration of oxygen microelectrodes is described. The tonometer equilibrated a fluid with a known gas tension within 30 s and has eliminated many problems previously associated with microelectrode evaluation.", "contents": "Tonometer for calibration and evaluation of oxygen microelectrodes. A temperature-controlled tonometer for the calibration of oxygen microelectrodes is described. The tonometer equilibrated a fluid with a known gas tension within 30 s and has eliminated many problems previously associated with microelectrode evaluation."}
{"id": "PMID:468601", "title": "Dynamic mechanisms determine functional residual capacity in mice, Mus musculus.", "content": "Awake mice (22.6--32.6 g) were anesthetized intravenously during head-out body plethysmography. One minute after pentobarbital sodium anesthesia, tidal volume had fallen from 0.28 +/- 0.04 to 0.14 +/- 0.02 ml and frequency from 181 +/- 20 to 142 +/- 8. Functional residual capacity (FRC) decreased by 0.10 +/- 0.02 ml. Expiratory flow-volume curves were linear, highly repeatable, and submaximal over substantial portions of expiration in awake and anesthetized mice; and expiration was interrupted at substantial flows that abruptly fell to and crossed zero as inspiration interrupted relaxed expiration. FRC is maintained at a higher level in awake mice due to a higher tidal volume and frequency coupled with expiratory braking (persistent inspiratory muscle activity or increased glottal resistance). In anesthetized mice, the absence of braking, coupled with reductions in tidal volume and frequency and a prolonged expiratory period, leads to FRCs that approach relaxation volume (Vr). An equation in derived to express the difference between FRC and Vr in terms of the portion of tidal volume expired without braking, the slope of the linear portion of the expiratory flow-volume curve expressed as V/V, the time fraction of one respiratory cycle spent in unbraked expiration, and respiratory frequency.", "contents": "Dynamic mechanisms determine functional residual capacity in mice, Mus musculus. Awake mice (22.6--32.6 g) were anesthetized intravenously during head-out body plethysmography. One minute after pentobarbital sodium anesthesia, tidal volume had fallen from 0.28 +/- 0.04 to 0.14 +/- 0.02 ml and frequency from 181 +/- 20 to 142 +/- 8. Functional residual capacity (FRC) decreased by 0.10 +/- 0.02 ml. Expiratory flow-volume curves were linear, highly repeatable, and submaximal over substantial portions of expiration in awake and anesthetized mice; and expiration was interrupted at substantial flows that abruptly fell to and crossed zero as inspiration interrupted relaxed expiration. FRC is maintained at a higher level in awake mice due to a higher tidal volume and frequency coupled with expiratory braking (persistent inspiratory muscle activity or increased glottal resistance). In anesthetized mice, the absence of braking, coupled with reductions in tidal volume and frequency and a prolonged expiratory period, leads to FRCs that approach relaxation volume (Vr). An equation in derived to express the difference between FRC and Vr in terms of the portion of tidal volume expired without braking, the slope of the linear portion of the expiratory flow-volume curve expressed as V/V, the time fraction of one respiratory cycle spent in unbraked expiration, and respiratory frequency."}
{"id": "PMID:468603", "title": "Ventilation and gas exchange during phasic hindlimb exercise in the dog.", "content": "To investigate the importance of the major neural afferent component from the exercising extremities in exercise hyperpnea, rhythmic contraction of hindlimb muscles was produced in the dog, by electrically stimulating the peripheral cut ends of the sciatic and femoral nerves, bilaterally, for 4- to 5-min periods. VE, VCO2, and VO2 were computed breath-by-breath and PaCO2 was monitored continuously with an indwelling arterial electrode. During exercise, VO2 and VCO2 were approximately doubled in the steady state, rising with t1/2 of 25 +/- 2 and 35 +/- 4 s, respectively. VE increased within five breaths after exercise onset, and thereafter rose to a steady state with a t1/2 of 37 +/- 5 s. Mean PaCO2 increased transiently within the 1st min of stimulation but was not significantly different from control in the steady state. We conclude that the major neural afferent component from the contracting muscles is not an obligatory requirement for normal ventilatory response in the steady state of phasic exercise.", "contents": "Ventilation and gas exchange during phasic hindlimb exercise in the dog. To investigate the importance of the major neural afferent component from the exercising extremities in exercise hyperpnea, rhythmic contraction of hindlimb muscles was produced in the dog, by electrically stimulating the peripheral cut ends of the sciatic and femoral nerves, bilaterally, for 4- to 5-min periods. VE, VCO2, and VO2 were computed breath-by-breath and PaCO2 was monitored continuously with an indwelling arterial electrode. During exercise, VO2 and VCO2 were approximately doubled in the steady state, rising with t1/2 of 25 +/- 2 and 35 +/- 4 s, respectively. VE increased within five breaths after exercise onset, and thereafter rose to a steady state with a t1/2 of 37 +/- 5 s. Mean PaCO2 increased transiently within the 1st min of stimulation but was not significantly different from control in the steady state. We conclude that the major neural afferent component from the contracting muscles is not an obligatory requirement for normal ventilatory response in the steady state of phasic exercise."}
{"id": "PMID:468604", "title": "Physical performance and peak aerobic power at different body temperatures.", "content": "In eight male subjects we studied the effect of different core (esophageal, (Tes 34.9--38.4 degrees C) and muscle (Tm 35.1--39.3 degrees C) temperature on 1) physical performance (time to exhaustion at a standard maximal rate of work, WT), 2) aerobic power (VO2), 3) heart rate (HR), and 4) blood lactate (LA) concentration during exhaustive combined arm and leg exercise. In three subjects the effects at different mean skin temperatures (Tsk 27 and 31 degrees C, respectively) were also studied. Peak VO2 was positively correlated to both Tes (r = 0.88) and Tm (r = 0.91). None of the subjects attained control VO2max at Tes and Tm lower than 37.5 and 38.0 degrees C, respectively. HR was correlated to both Tes (r = 0.97) and Tm (r = 0.95). Different Tsk did not affect peak VO2 and HR at subnormal body temperatures. Pulmonary ventilation was independent of Tes and Tm in all experimental situations. LA was significantly higher at Tes 37.5 degrees C compared to both Tes 34.9 and 38.5 degrees C, respectively. At Tes less than 37.5 degrees C and Tm less than 38.0 degrees C, there was a linear reduction in WT (20%.degrees C-1), peak VO2 (5--6%.degrees C-1), and HR (8 beats.min-1.degrees C-1) with lowered Tes and Tm.", "contents": "Physical performance and peak aerobic power at different body temperatures. In eight male subjects we studied the effect of different core (esophageal, (Tes 34.9--38.4 degrees C) and muscle (Tm 35.1--39.3 degrees C) temperature on 1) physical performance (time to exhaustion at a standard maximal rate of work, WT), 2) aerobic power (VO2), 3) heart rate (HR), and 4) blood lactate (LA) concentration during exhaustive combined arm and leg exercise. In three subjects the effects at different mean skin temperatures (Tsk 27 and 31 degrees C, respectively) were also studied. Peak VO2 was positively correlated to both Tes (r = 0.88) and Tm (r = 0.91). None of the subjects attained control VO2max at Tes and Tm lower than 37.5 and 38.0 degrees C, respectively. HR was correlated to both Tes (r = 0.97) and Tm (r = 0.95). Different Tsk did not affect peak VO2 and HR at subnormal body temperatures. Pulmonary ventilation was independent of Tes and Tm in all experimental situations. LA was significantly higher at Tes 37.5 degrees C compared to both Tes 34.9 and 38.5 degrees C, respectively. At Tes less than 37.5 degrees C and Tm less than 38.0 degrees C, there was a linear reduction in WT (20%.degrees C-1), peak VO2 (5--6%.degrees C-1), and HR (8 beats.min-1.degrees C-1) with lowered Tes and Tm."}
{"id": "PMID:468605", "title": "Effects of extrapulmonary airway distension on breathing in anesthetized dogs.", "content": "Anesthetized dogs with open chests were perfused with a pump-oxygenator. We first observed the effects of intermittent periods of sustained inflation of both lungs, of only one lobe, and of an intra-airway balloon on the diaphragm electromyogram (EMG). Lung or lobe inflation with low pressures depressed respiratory frequency (fr) by prolonging expiratory time (TE) in proportion to inflating pressure, but balloon inflation was without effect. We next intermittently inflated one or two lobes after all others were ligated and thereafter inflated all the extrapulmonary airways following total lung excision. Lobe inflation, by varying TE, decreased fr at low pressures and often increased fr at higher pressures. Extrapulmonary airway distension slightly increased fr and the mean rate of rise of the integrated EMG, but decreased inspiratory time and peak EMG amplitude. TE was slightly prolonged by low pressures when using sustained airway pressurization but was shortened by higher pressures when distending cyclically. In comparison to lobe inflation, the effects from isolated airways were small, and the inhibitory effect of lung inflation could not be attributed to them.", "contents": "Effects of extrapulmonary airway distension on breathing in anesthetized dogs. Anesthetized dogs with open chests were perfused with a pump-oxygenator. We first observed the effects of intermittent periods of sustained inflation of both lungs, of only one lobe, and of an intra-airway balloon on the diaphragm electromyogram (EMG). Lung or lobe inflation with low pressures depressed respiratory frequency (fr) by prolonging expiratory time (TE) in proportion to inflating pressure, but balloon inflation was without effect. We next intermittently inflated one or two lobes after all others were ligated and thereafter inflated all the extrapulmonary airways following total lung excision. Lobe inflation, by varying TE, decreased fr at low pressures and often increased fr at higher pressures. Extrapulmonary airway distension slightly increased fr and the mean rate of rise of the integrated EMG, but decreased inspiratory time and peak EMG amplitude. TE was slightly prolonged by low pressures when using sustained airway pressurization but was shortened by higher pressures when distending cyclically. In comparison to lobe inflation, the effects from isolated airways were small, and the inhibitory effect of lung inflation could not be attributed to them."}
{"id": "PMID:468606", "title": "Fatigue of inspiratory muscles and their synergic behavior.", "content": "The time (tlim) required to produce inspiratory muscle fatigue was measured in five normal subjects breathing at functional residual capacity (FRC) against a variety of high inspiratory resistive loads. In every breathing test the subjects generated with each inspiration a mouth pressure (Pm) that was a predetermined fraction of maximum Pm (Pmmax). They continued breathing until they were unable to generate this Pm. The Pm/Pmmax that could be generated indefinitely (Pmcrit) was around 60%. The inspiratory power output at that level of breathing was 6.6 kg.m/min (Wcrit). In three of those subjects the same experiment was conducted at an end-expiratory volume of FRC + one-half inspiratory capacity (1/2IC). The higher lung volume was actively maintained by the subjects watching end-expiratory transpulmonary pressure on an oscilloscope. For any fraction of the maximum mouth pressure at FRC + 1/2IC (Pm'max), tlim was shorter than FRC. Pmcrit decreased to 30% Pm'max and Wcrit to 2.6 kg.m/min. Monitoring the abdominal pressure revealed that the contribution of the diaphragm and intercostal accessory muscles alternated in time, possibly postponing the onset of fatigue.", "contents": "Fatigue of inspiratory muscles and their synergic behavior. The time (tlim) required to produce inspiratory muscle fatigue was measured in five normal subjects breathing at functional residual capacity (FRC) against a variety of high inspiratory resistive loads. In every breathing test the subjects generated with each inspiration a mouth pressure (Pm) that was a predetermined fraction of maximum Pm (Pmmax). They continued breathing until they were unable to generate this Pm. The Pm/Pmmax that could be generated indefinitely (Pmcrit) was around 60%. The inspiratory power output at that level of breathing was 6.6 kg.m/min (Wcrit). In three of those subjects the same experiment was conducted at an end-expiratory volume of FRC + one-half inspiratory capacity (1/2IC). The higher lung volume was actively maintained by the subjects watching end-expiratory transpulmonary pressure on an oscilloscope. For any fraction of the maximum mouth pressure at FRC + 1/2IC (Pm'max), tlim was shorter than FRC. Pmcrit decreased to 30% Pm'max and Wcrit to 2.6 kg.m/min. Monitoring the abdominal pressure revealed that the contribution of the diaphragm and intercostal accessory muscles alternated in time, possibly postponing the onset of fatigue."}
{"id": "PMID:468607", "title": "Bradycardia during face cooling in man may be produced by selective brain cooling.", "content": "In human subjects, bradycardia was produced by immersing the subjects' faces in water at 15 degrees C when they were hyperthermic. When they were hypothermic, the same face cooling produced tachycardia. It is suggested that the difference in cardiac response originates in selective brain cooling during hyperthermia, by venous return from the face to the brain, via ophthalmic veins.", "contents": "Bradycardia during face cooling in man may be produced by selective brain cooling. In human subjects, bradycardia was produced by immersing the subjects' faces in water at 15 degrees C when they were hyperthermic. When they were hypothermic, the same face cooling produced tachycardia. It is suggested that the difference in cardiac response originates in selective brain cooling during hyperthermia, by venous return from the face to the brain, via ophthalmic veins."}
{"id": "PMID:468608", "title": "Decreased exercise hyperpnea in patients with bilateral carotid chemoreceptor resection.", "content": "Exercise hyperpnea was compared in 5 asthmatics 25 yr after bilateral carotid body resection (BR), 4 others 19 yr after unilateral resection (UR), and 12 controls (C) matched for age and pulmonary flow limitation. In the BR group, ventilation rose less with exercise, mostly because BR experienced less tachypnea. End-tidal PCO2 rose 5.8 +/- 3.2 (P less than 0.05) to 46 Torr at 50 W. In UR and C the same load did not increase PETCO2 significantly (+2.1 and +1.4 Torr, respectively). Arterial-end-tidal PCO2 differences before and 15--45 s postexercise were insignificant in all three groups. Heart rate and blood pressure rose equally in the three groups, suggesting that the ventilatory effects were not secondary to blood flow differences and disclosing no evidence of baroreceptor denervation during glomectomy.", "contents": "Decreased exercise hyperpnea in patients with bilateral carotid chemoreceptor resection. Exercise hyperpnea was compared in 5 asthmatics 25 yr after bilateral carotid body resection (BR), 4 others 19 yr after unilateral resection (UR), and 12 controls (C) matched for age and pulmonary flow limitation. In the BR group, ventilation rose less with exercise, mostly because BR experienced less tachypnea. End-tidal PCO2 rose 5.8 +/- 3.2 (P less than 0.05) to 46 Torr at 50 W. In UR and C the same load did not increase PETCO2 significantly (+2.1 and +1.4 Torr, respectively). Arterial-end-tidal PCO2 differences before and 15--45 s postexercise were insignificant in all three groups. Heart rate and blood pressure rose equally in the three groups, suggesting that the ventilatory effects were not secondary to blood flow differences and disclosing no evidence of baroreceptor denervation during glomectomy."}
{"id": "PMID:468609", "title": "Lung volume and pleural pressure in the anesthetized hamster.", "content": "Lung volumes and respiratory pressures were measured in anesthetized male hamsters weighing an average 117 g. In 16 supine animals functional residual capacity (FRC) determined by body plethysmograph was 1.12 +/- 0.23 (SD) ml (about 20% total lung capacity, TLC) slightly and significantly larger than the FRC measured by saline displacement, 1.01 +/- 0.15 ml. Similar results were found in six prone animals. Paralysis did not significantly alter supine FRC. Contrary to published reports, pleural pressure (Ppl) estimated from esophageal pressure was negative at FRC. The fact that lung volume decreased by 0.2 ml (about 4% TLC) when the chest was opened at FRC provided additional evidence of negative Ppl at FRC. No consistent changes in the lung pressure-volume curve were found after the chest was opened. Deflation chest wall compliance just above FRC was about twice lung compliance. The vital capacity and reserve volumes in this study agreed with values reported in the literature. However, absolute lung volumes (TLC, FRC, and residual volume) were lower by about 1.4 ml, possibly because of earlier overestimates of box FRC.", "contents": "Lung volume and pleural pressure in the anesthetized hamster. Lung volumes and respiratory pressures were measured in anesthetized male hamsters weighing an average 117 g. In 16 supine animals functional residual capacity (FRC) determined by body plethysmograph was 1.12 +/- 0.23 (SD) ml (about 20% total lung capacity, TLC) slightly and significantly larger than the FRC measured by saline displacement, 1.01 +/- 0.15 ml. Similar results were found in six prone animals. Paralysis did not significantly alter supine FRC. Contrary to published reports, pleural pressure (Ppl) estimated from esophageal pressure was negative at FRC. The fact that lung volume decreased by 0.2 ml (about 4% TLC) when the chest was opened at FRC provided additional evidence of negative Ppl at FRC. No consistent changes in the lung pressure-volume curve were found after the chest was opened. Deflation chest wall compliance just above FRC was about twice lung compliance. The vital capacity and reserve volumes in this study agreed with values reported in the literature. However, absolute lung volumes (TLC, FRC, and residual volume) were lower by about 1.4 ml, possibly because of earlier overestimates of box FRC."}
{"id": "PMID:468610", "title": "Regional extravascular lung water in normal sheep.", "content": "We measured the regional distribution of pulmonary extravascular water to test our prediction that, because of higher vascular hydrostatic pressure in more dependent zones, the bottom of the lung would tend to be wetter than the top. We injected eight normal sheep under halothane anesthesia with 125I-labeled albumin and killed them 5 min later. We suspended the sheep in the prone position and froze them solid in dry ice. We sawed the thorax into horizontal slices, chipped the frozen lung from each, and determined extravascular lung water and hematocrit. Hematocrit was calculated from separately measured red blood cell (tissue hemoglobin) and plasma (125I-albumin) masses. We found regional extravascular water was constant throughout the lung. Regional hematocrit was significantly higher at the lung base than at the apex in these slowly frozen sheep after death. Calculation of extravascular water using a single blood mass marker (hemoglobin) underestimated lung water, more so at the base than at the apex, because blood mass was overestimated. Accurate measurement of blood mass is critical in the calculation of regional lung water.", "contents": "Regional extravascular lung water in normal sheep. We measured the regional distribution of pulmonary extravascular water to test our prediction that, because of higher vascular hydrostatic pressure in more dependent zones, the bottom of the lung would tend to be wetter than the top. We injected eight normal sheep under halothane anesthesia with 125I-labeled albumin and killed them 5 min later. We suspended the sheep in the prone position and froze them solid in dry ice. We sawed the thorax into horizontal slices, chipped the frozen lung from each, and determined extravascular lung water and hematocrit. Hematocrit was calculated from separately measured red blood cell (tissue hemoglobin) and plasma (125I-albumin) masses. We found regional extravascular water was constant throughout the lung. Regional hematocrit was significantly higher at the lung base than at the apex in these slowly frozen sheep after death. Calculation of extravascular water using a single blood mass marker (hemoglobin) underestimated lung water, more so at the base than at the apex, because blood mass was overestimated. Accurate measurement of blood mass is critical in the calculation of regional lung water."}
{"id": "PMID:468612", "title": "Glucose metabolism in rat lung during exposure to hyperbaric O2.", "content": "Lung metabolism on exposure to hyperbaric oxygen was studied in rat lungs perfused with artificial media and ventilated with O2 at 0.2, 1, or 5 ATA. During the first 80 min of exposure to O2 at 5 ATA, glucose utilization increased 55%, lactate plus pyruvate production increased 45%, total CO2 production increased 47%, tissue ATP decreased 17%, and the ATP/ADP decreased 29% compared with 0.2 ATA O2. The increased CO2 production was due to a nearly twofold stimulation of pentose cycle activity whereas mitochondrial CO2 production did not change significantly. There were no significant differences in metabolism between lungs studied at 0.2 and 1 ATA O2. During the next hour of perfusion, there was a marked increase in mitochondrial CO2 production of control lungs but tissue ATP/ADP did not change significantly. With oxygen at 5 ATA, mitochondrial CO2 production increased only slightly, tissue ATP/ADP decreased further, and lungs demonstrated accumulation of edema fluid. The results indicate that exposure of lungs to hyperbaric oxygen results in stimulation of NADPH turnover through the pentose cycle and increased ATP generation, although the increased rate was not sufficient to maintain normal ATP/ADP.", "contents": "Glucose metabolism in rat lung during exposure to hyperbaric O2. Lung metabolism on exposure to hyperbaric oxygen was studied in rat lungs perfused with artificial media and ventilated with O2 at 0.2, 1, or 5 ATA. During the first 80 min of exposure to O2 at 5 ATA, glucose utilization increased 55%, lactate plus pyruvate production increased 45%, total CO2 production increased 47%, tissue ATP decreased 17%, and the ATP/ADP decreased 29% compared with 0.2 ATA O2. The increased CO2 production was due to a nearly twofold stimulation of pentose cycle activity whereas mitochondrial CO2 production did not change significantly. There were no significant differences in metabolism between lungs studied at 0.2 and 1 ATA O2. During the next hour of perfusion, there was a marked increase in mitochondrial CO2 production of control lungs but tissue ATP/ADP did not change significantly. With oxygen at 5 ATA, mitochondrial CO2 production increased only slightly, tissue ATP/ADP decreased further, and lungs demonstrated accumulation of edema fluid. The results indicate that exposure of lungs to hyperbaric oxygen results in stimulation of NADPH turnover through the pentose cycle and increased ATP generation, although the increased rate was not sufficient to maintain normal ATP/ADP."}
{"id": "PMID:468613", "title": "Perivascular pressure measurements by wick-catheter technique in isolated dog lobes.", "content": "Saline-filled wick catheters were inserted intraparenchymally in the loose connective tissue separating the bronchus and pulmonary artery of unperfused isolated dog lobes. Px, the difference between perivascular pressure and pleural surface pressure (which was atmospheric pressure), was measured by the wick for arterial pressures (Pa) of 10 and 25 cmH2O at lobe transpulmonary pressures (Ptp) of 25, 15, 10, 5, and 2 cmH2O during a deflation pressure-volume maneuver. The response time of Px to step changes in Pa was relatively short, a pressure plateau always occurring in less than 3 min. For Pa of 25 cmH2O, mean Px in five lobes was -2 cmH2O at Ptp of 2 cmH2O and decreased almost linearly to -9 cmH2O at Ptp of 25 cmH2O. Reducing Pa from 25 to 10 cmH2O resulted in a mean decrease in Px of 1 cmH2O at Ptp of 2 cmH2O and 2 cmH2O at Ptp of 25 cmH2O. These results are generally consistent with predictions from a continuum-mechanics analysis of pulmonary vascular interdependence and do not support the concept that perivascular interstitial fluid pressure is different from surface pressure.", "contents": "Perivascular pressure measurements by wick-catheter technique in isolated dog lobes. Saline-filled wick catheters were inserted intraparenchymally in the loose connective tissue separating the bronchus and pulmonary artery of unperfused isolated dog lobes. Px, the difference between perivascular pressure and pleural surface pressure (which was atmospheric pressure), was measured by the wick for arterial pressures (Pa) of 10 and 25 cmH2O at lobe transpulmonary pressures (Ptp) of 25, 15, 10, 5, and 2 cmH2O during a deflation pressure-volume maneuver. The response time of Px to step changes in Pa was relatively short, a pressure plateau always occurring in less than 3 min. For Pa of 25 cmH2O, mean Px in five lobes was -2 cmH2O at Ptp of 2 cmH2O and decreased almost linearly to -9 cmH2O at Ptp of 25 cmH2O. Reducing Pa from 25 to 10 cmH2O resulted in a mean decrease in Px of 1 cmH2O at Ptp of 2 cmH2O and 2 cmH2O at Ptp of 25 cmH2O. These results are generally consistent with predictions from a continuum-mechanics analysis of pulmonary vascular interdependence and do not support the concept that perivascular interstitial fluid pressure is different from surface pressure."}
{"id": "PMID:468614", "title": "A direct-display oscillation method for measurement of respiratory impedance.", "content": "The basic principle of the method described here is derived from a variant of the oscillation method. A reference impedance is connected to the mouth; between these two an oscillating flow is imposed. As a reference impedance we use a flexible tube, which acts as a virtually pure inductance or inertance. Respiration is hardly impeded. The only measured parameter is the alternating pressure in front of the mouth and this is easily picked up by a simple microphone. In contrast to former direct-display methods, the inertia and elasticity of the respiratory gas and the respiratory tract, i.e., airways including lungs and thorax, are also taken into account for the evaluation. The respiratory resistance is studied as a complex parameters, i.e., as an impedance. With the aid of diagrams or via electronic computation circuitry, the direct and continuous display of all impedance components such as its magnitude and phase, resistance, and reactance is possible. They can be read out as a function of time, respiratory flow, or volume.", "contents": "A direct-display oscillation method for measurement of respiratory impedance. The basic principle of the method described here is derived from a variant of the oscillation method. A reference impedance is connected to the mouth; between these two an oscillating flow is imposed. As a reference impedance we use a flexible tube, which acts as a virtually pure inductance or inertance. Respiration is hardly impeded. The only measured parameter is the alternating pressure in front of the mouth and this is easily picked up by a simple microphone. In contrast to former direct-display methods, the inertia and elasticity of the respiratory gas and the respiratory tract, i.e., airways including lungs and thorax, are also taken into account for the evaluation. The respiratory resistance is studied as a complex parameters, i.e., as an impedance. With the aid of diagrams or via electronic computation circuitry, the direct and continuous display of all impedance components such as its magnitude and phase, resistance, and reactance is possible. They can be read out as a function of time, respiratory flow, or volume."}
{"id": "PMID:468615", "title": "Effects of methacholine and hypocapnia on airways and collateral ventilation in dogs.", "content": "We studied the effects of hypocapnia and methacholine on small airways resistance (Rsaw) and collateral ventilation in anesthetized paralyzed dogs. The animals were ventilated with air while either 10% CO2 or air (hypocapnia) was infused through a segment obstructed with a fiber-optic bronchoscope. Measurements were made before and after instillation of methacholine into the obstructed segment. Collateral resistance (Rcoll) and Rsaw increased with hypocapnia and methacholine. The time constant for collateral ventilation increased with hypocapnia, but did not change with methacholine because of decreases in the compliance of the obstructed segment. We conclude that collateral channels respond to methacholine and hypocapnia in a manner similar to small airways and that local parasympathomimetic stimulation, unlike lung deflation does not increase the time constant for collateral ventilation.", "contents": "Effects of methacholine and hypocapnia on airways and collateral ventilation in dogs. We studied the effects of hypocapnia and methacholine on small airways resistance (Rsaw) and collateral ventilation in anesthetized paralyzed dogs. The animals were ventilated with air while either 10% CO2 or air (hypocapnia) was infused through a segment obstructed with a fiber-optic bronchoscope. Measurements were made before and after instillation of methacholine into the obstructed segment. Collateral resistance (Rcoll) and Rsaw increased with hypocapnia and methacholine. The time constant for collateral ventilation increased with hypocapnia, but did not change with methacholine because of decreases in the compliance of the obstructed segment. We conclude that collateral channels respond to methacholine and hypocapnia in a manner similar to small airways and that local parasympathomimetic stimulation, unlike lung deflation does not increase the time constant for collateral ventilation."}
{"id": "PMID:468616", "title": "Circulatory response to arterial hyperoxia.", "content": "We have studied the circulatory response to 100% O2 at 1 and 3 atm, using unanesthetized rabbits in which a systemic artery and the right heart had been cannulated previously. One group of animals served as controls; the other was infused with a flurocarbon emulsion that boosted blood O2 solubility to approximately 5 ml.100 ml-1.atm-1. Exposure to hyperoxia caused an identical sustained rise in arterial PO2 in both groups. O2 uptake was measured during normobaric exposure to 100% O2 and was found to be the same as in control conditions. There was an immediate rise in right heart PO2, more marked in infused animals, but this increase was only temporary, and PO2 dropped, while the right heart-arterial PCO2 difference rose, indicating a gradual fall in cardiac output. This readjustment occurred at a faster rate in the infused animals, a difference that led us to conclude that the peripheral response to hyperoxia is influenced by factors other than arterial PO2. The pronounced decrease in cardiac output seen in infused rabbits was accompanied by lactic acidosis, implying that some of the animals' tissues were becoming hypoxic in the presence of arterial hyperoxia.", "contents": "Circulatory response to arterial hyperoxia. We have studied the circulatory response to 100% O2 at 1 and 3 atm, using unanesthetized rabbits in which a systemic artery and the right heart had been cannulated previously. One group of animals served as controls; the other was infused with a flurocarbon emulsion that boosted blood O2 solubility to approximately 5 ml.100 ml-1.atm-1. Exposure to hyperoxia caused an identical sustained rise in arterial PO2 in both groups. O2 uptake was measured during normobaric exposure to 100% O2 and was found to be the same as in control conditions. There was an immediate rise in right heart PO2, more marked in infused animals, but this increase was only temporary, and PO2 dropped, while the right heart-arterial PCO2 difference rose, indicating a gradual fall in cardiac output. This readjustment occurred at a faster rate in the infused animals, a difference that led us to conclude that the peripheral response to hyperoxia is influenced by factors other than arterial PO2. The pronounced decrease in cardiac output seen in infused rabbits was accompanied by lactic acidosis, implying that some of the animals' tissues were becoming hypoxic in the presence of arterial hyperoxia."}
{"id": "PMID:468617", "title": "Control of respiratory evaporative heat loss in exercising goats.", "content": "Investigations were carried out to determine whether a nonthermal input is involved in the control of respiratory evaporative heat loss (REHL) in exercising goats. Two goats were implanted with hypothalamic perfusion thermodes and three goats were implanted with intravascular heat exchangers to clamp hypothalamic temperature and total body core temperature, respectively. At 30 degrees C air temperature REHL was measured while the animals were resting or walking on a treadmill (3 km.h-1, 5 degrees gradient). When the hypothalamic temperature was clamped between 33.0 and 43.0 degrees C the slopes of the responses relating increased REHL to hypothalamic temperature were similar during rest and exercise. However, the threshold hypothalamic temperatures for the increased REHL responses were lower during exercise than at rest, presumably due to higher extrahypothalamic temperatures. When the body core temperature was clamped between 37.0 and 40.4 degrees C the slopes of the responses relating increased REHL to total body core temperature during exercise showed only minor differences compared to those at rest, none of them conclusively indicating nonthermal influences.", "contents": "Control of respiratory evaporative heat loss in exercising goats. Investigations were carried out to determine whether a nonthermal input is involved in the control of respiratory evaporative heat loss (REHL) in exercising goats. Two goats were implanted with hypothalamic perfusion thermodes and three goats were implanted with intravascular heat exchangers to clamp hypothalamic temperature and total body core temperature, respectively. At 30 degrees C air temperature REHL was measured while the animals were resting or walking on a treadmill (3 km.h-1, 5 degrees gradient). When the hypothalamic temperature was clamped between 33.0 and 43.0 degrees C the slopes of the responses relating increased REHL to hypothalamic temperature were similar during rest and exercise. However, the threshold hypothalamic temperatures for the increased REHL responses were lower during exercise than at rest, presumably due to higher extrahypothalamic temperatures. When the body core temperature was clamped between 37.0 and 40.4 degrees C the slopes of the responses relating increased REHL to total body core temperature during exercise showed only minor differences compared to those at rest, none of them conclusively indicating nonthermal influences."}
{"id": "PMID:468618", "title": "Effect of static or slowly flowing blood on carbon monoxide diffusion in dog lungs.", "content": "During temporary left pulmonary artery occlusion (TLPAO) in dogs, blood in pulmonary capillaries downstream from the occlusion is static or flowing slowly. In such areas, the uptake of carbon monoxide (CO) and diffusing capacity (DLCO) should decrease with time as carboxyhemoglobin concentration increases. We measured DLCO during exhalation of five sequential breaths in anesthetized dogs using a modification of a technique recently described in our laboratory (J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 43: 617--625, 1977). During TLPAO, the decrease in DLCO was greatest at low lung volumes, suggesting that the occluded lung was emptying later in exhalation, a conclusion supported by measurements of DLCO during TLPAO with the right mainstem bronchus occluded. In addition. DLCO decreased with each breath as the backpressure to diffusion increased in static capillary blood. Inhalation of 4% CO accelerated the rate of decrease in DLCO. Measurement of DLCO during exhalation over multiple breaths may help detect pulmonary vascular obstruction.", "contents": "Effect of static or slowly flowing blood on carbon monoxide diffusion in dog lungs. During temporary left pulmonary artery occlusion (TLPAO) in dogs, blood in pulmonary capillaries downstream from the occlusion is static or flowing slowly. In such areas, the uptake of carbon monoxide (CO) and diffusing capacity (DLCO) should decrease with time as carboxyhemoglobin concentration increases. We measured DLCO during exhalation of five sequential breaths in anesthetized dogs using a modification of a technique recently described in our laboratory (J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 43: 617--625, 1977). During TLPAO, the decrease in DLCO was greatest at low lung volumes, suggesting that the occluded lung was emptying later in exhalation, a conclusion supported by measurements of DLCO during TLPAO with the right mainstem bronchus occluded. In addition. DLCO decreased with each breath as the backpressure to diffusion increased in static capillary blood. Inhalation of 4% CO accelerated the rate of decrease in DLCO. Measurement of DLCO during exhalation over multiple breaths may help detect pulmonary vascular obstruction."}
{"id": "PMID:468619", "title": "Stimulation of phrenic nerve activity by salicylate.", "content": "To assess the possibility that salicylate stimulates VE by direct excitation of phrenic motoneurons, we compared two groups of anesthetized vagotomized dogs with respect to increases in phrenic nerve activity elicited by a large dose of sodium salicylate (225 mg/kg). The sole difference between the two groups of animals was the condition of the spinal cord (SC); SC remained intact in one group of animals (i.e., intact animals) whereas the other group of animals (i.e., T1 spinal-transected animals) underwent complete transection of the SC at the first thoracic level. Both groups of animals were ventilated by a respirator and arterial PCO2 was maintained constant throughout all experiments. Following salicylate infusion, intact animals exhibited two- to threefold increases in the frequency of phrenic nerve bursts and three- to fivefold increases in moving average minute phrenic activity (i.e., the summation of peak integrated burst activity per minute). In contrast, salicylate infusion into T1 spinal-transected animals elicited no statistically significant increase in the frequency of phrenic nerve bursts while increases in minute phrenic activity were limited to 32 +/- 8%. Since T1 spinal transection markedly diminishes increases in phrenic nerve activity elicited by salicylate, we conclude that salicylate stimulates VE by a reflex mechanism whose afferent pathways originate in metameres below T1.", "contents": "Stimulation of phrenic nerve activity by salicylate. To assess the possibility that salicylate stimulates VE by direct excitation of phrenic motoneurons, we compared two groups of anesthetized vagotomized dogs with respect to increases in phrenic nerve activity elicited by a large dose of sodium salicylate (225 mg/kg). The sole difference between the two groups of animals was the condition of the spinal cord (SC); SC remained intact in one group of animals (i.e., intact animals) whereas the other group of animals (i.e., T1 spinal-transected animals) underwent complete transection of the SC at the first thoracic level. Both groups of animals were ventilated by a respirator and arterial PCO2 was maintained constant throughout all experiments. Following salicylate infusion, intact animals exhibited two- to threefold increases in the frequency of phrenic nerve bursts and three- to fivefold increases in moving average minute phrenic activity (i.e., the summation of peak integrated burst activity per minute). In contrast, salicylate infusion into T1 spinal-transected animals elicited no statistically significant increase in the frequency of phrenic nerve bursts while increases in minute phrenic activity were limited to 32 +/- 8%. Since T1 spinal transection markedly diminishes increases in phrenic nerve activity elicited by salicylate, we conclude that salicylate stimulates VE by a reflex mechanism whose afferent pathways originate in metameres below T1."}
{"id": "PMID:468620", "title": "Anaerobic threshold alterations caused by endurance training in middle-aged men.", "content": "Nine previously sedentary middle-aged males underwent cycle endurance training 45 min/day for 9 wk with an average attendance of 4.1 days/wk. Seven males served as controls. Before and after the training period, the subjects performed three cycle ergometer tests. Work rate was incremented by 15 W/min, to the limit of the subjects' tolerance, in the first two tests; the third test consisted of contant-load cycling at an O2 uptake (VO2) just below the pretraining anaerobic threshold (AT). After training, the AT increased significantly by 44%, expressed as absolute VO2, and by 15%, expressed relative to VO2 max. Significant increases were also noted in VO2max (25%), maximal minute ventilation (19%), and maximal work rate (28%). The test-retest correlation coefficients for the AT (%VO2max) were 0.91, pre- and posttraining. Training did not alter steady-state VO2 during the submaximal exercise test whereas significant decreases occurred in CO2 output, VE, respiratory quotient, and VE/VO2. No changes occurred in the control subjects during this period. These results demonstrate that the AT is profoundly influenced by endurance training in previously sedentary middle-aged males.", "contents": "Anaerobic threshold alterations caused by endurance training in middle-aged men. Nine previously sedentary middle-aged males underwent cycle endurance training 45 min/day for 9 wk with an average attendance of 4.1 days/wk. Seven males served as controls. Before and after the training period, the subjects performed three cycle ergometer tests. Work rate was incremented by 15 W/min, to the limit of the subjects' tolerance, in the first two tests; the third test consisted of contant-load cycling at an O2 uptake (VO2) just below the pretraining anaerobic threshold (AT). After training, the AT increased significantly by 44%, expressed as absolute VO2, and by 15%, expressed relative to VO2 max. Significant increases were also noted in VO2max (25%), maximal minute ventilation (19%), and maximal work rate (28%). The test-retest correlation coefficients for the AT (%VO2max) were 0.91, pre- and posttraining. Training did not alter steady-state VO2 during the submaximal exercise test whereas significant decreases occurred in CO2 output, VE, respiratory quotient, and VE/VO2. No changes occurred in the control subjects during this period. These results demonstrate that the AT is profoundly influenced by endurance training in previously sedentary middle-aged males."}
{"id": "PMID:468621", "title": "Mechanism of decreased blood flow to atelectatic lung.", "content": "This study examined the relative contribution of passive mechanical forces vs. hypoxic pulmonary vasoconstriction as mechanisms of blood flow reduction through atelectatic canine lung. Selective atelectasis of the left lower lobe caused the electromagnetically measured lobar blood flow to decrease 59% from control levels. Reexpansion and ventilation of the left lower lobe with 95% N2-5% CO2, which should terminate any passive mechanical contribution to the decreased test lobe blood flow, did not cause any significant increase in left lower lobe blood flow. Ventilation of the left lower lobe with 100% O2, which should terminate any hypoxic pulmonary vasoconstriction contribution to the decreased test lobe blood flow, increased blood flow back to levels not significantly different from control. Differences between degree of hypoxia, magnitude of transpulmonary pressure, and absolute pulmonary vascular pressure during left lower lobe atelectasis and ventilation with N2 were considered to be minor influences. I conclude that the mechanism of decreased blood flow to an atelectatic lobe is hypoxic pulmonary vasoconstriction.", "contents": "Mechanism of decreased blood flow to atelectatic lung. This study examined the relative contribution of passive mechanical forces vs. hypoxic pulmonary vasoconstriction as mechanisms of blood flow reduction through atelectatic canine lung. Selective atelectasis of the left lower lobe caused the electromagnetically measured lobar blood flow to decrease 59% from control levels. Reexpansion and ventilation of the left lower lobe with 95% N2-5% CO2, which should terminate any passive mechanical contribution to the decreased test lobe blood flow, did not cause any significant increase in left lower lobe blood flow. Ventilation of the left lower lobe with 100% O2, which should terminate any hypoxic pulmonary vasoconstriction contribution to the decreased test lobe blood flow, increased blood flow back to levels not significantly different from control. Differences between degree of hypoxia, magnitude of transpulmonary pressure, and absolute pulmonary vascular pressure during left lower lobe atelectasis and ventilation with N2 were considered to be minor influences. I conclude that the mechanism of decreased blood flow to an atelectatic lobe is hypoxic pulmonary vasoconstriction."}
{"id": "PMID:468622", "title": "Thermoregulation in unrestrained rats during and after exposure to 1.5--4 G.", "content": "Unrestrained rats were exposed to cold for 1 h during and immediately after exposure to hypergravic fields (1.5--4 G) to determine if they recover their ability to thermoregulate on reentry to 1-G conditions. In contrast to the decreased body temperatures observed when cold exposure occurred concurrently with acceleration, hypothalamic, carotid, and brown fat temperatures did not fall when rats were exposed to cold immediately after return to 1 G. These results support the hypothesis that the thermoregulatory alterations seen under hypergravic conditions are manifestations of an effect of ongoing exposure to hypergravity and can be reversed on termination of acceleration. The reversibility of the thermoregulatory impairment is apparently unaffected by the magnitude of the acceleration field over a range of 1.5--4 G.", "contents": "Thermoregulation in unrestrained rats during and after exposure to 1.5--4 G. Unrestrained rats were exposed to cold for 1 h during and immediately after exposure to hypergravic fields (1.5--4 G) to determine if they recover their ability to thermoregulate on reentry to 1-G conditions. In contrast to the decreased body temperatures observed when cold exposure occurred concurrently with acceleration, hypothalamic, carotid, and brown fat temperatures did not fall when rats were exposed to cold immediately after return to 1 G. These results support the hypothesis that the thermoregulatory alterations seen under hypergravic conditions are manifestations of an effect of ongoing exposure to hypergravity and can be reversed on termination of acceleration. The reversibility of the thermoregulatory impairment is apparently unaffected by the magnitude of the acceleration field over a range of 1.5--4 G."}
{"id": "PMID:468623", "title": "Determinants of gas exchange kinetics during exercise in the dog.", "content": "Following exercise onset, CO2 output (VCO2) and O2 uptake (VO2) increase exponentially, but with appreciably different time constants. To determine the sensitivity of the time courses of these variables to altered ventilatory kinetics, rhythmic exercise was induced abruptly in anesthetized dogs by bilateral stimulation of the peripheral ends of the cut sciatic and femoral nerves. This increased the metabolic rate by 83 +/- 25 (SD) %. The dogs were ventilated with a constant-volume pump, the frequency of which was changed exponentially from the start of the exercise up to the ventilation that returned arterial CO2 and O2 pressure (PCO2 and PO2) in the steady state to resting levels. The time constant (tau) of  the increase in ventilation (VE) was varied among trials. VCO2, VO2, end-tidal PCO2 and PO2, and arterial PCO2 were measured breath by breath. tauVO2 was constant at approximately 18 s regardless of alterations in tauVE. In contrast, tauVCO2 was strongly dependent on tauVE, apparently due to the larger body stores for CO2; the transitions were isocapnic when tau VE was approximately 40 s. We conclude that ventilatory dynamics can markedly influence the dynamics of CO2 exchange during exercise, but has no appreciable effect on O2 uptake dynamics.", "contents": "Determinants of gas exchange kinetics during exercise in the dog. Following exercise onset, CO2 output (VCO2) and O2 uptake (VO2) increase exponentially, but with appreciably different time constants. To determine the sensitivity of the time courses of these variables to altered ventilatory kinetics, rhythmic exercise was induced abruptly in anesthetized dogs by bilateral stimulation of the peripheral ends of the cut sciatic and femoral nerves. This increased the metabolic rate by 83 +/- 25 (SD) %. The dogs were ventilated with a constant-volume pump, the frequency of which was changed exponentially from the start of the exercise up to the ventilation that returned arterial CO2 and O2 pressure (PCO2 and PO2) in the steady state to resting levels. The time constant (tau) of  the increase in ventilation (VE) was varied among trials. VCO2, VO2, end-tidal PCO2 and PO2, and arterial PCO2 were measured breath by breath. tauVO2 was constant at approximately 18 s regardless of alterations in tauVE. In contrast, tauVCO2 was strongly dependent on tauVE, apparently due to the larger body stores for CO2; the transitions were isocapnic when tau VE was approximately 40 s. We conclude that ventilatory dynamics can markedly influence the dynamics of CO2 exchange during exercise, but has no appreciable effect on O2 uptake dynamics."}
{"id": "PMID:468624", "title": "Thermal increment provided by inhalation rewarming from hypothermia.", "content": "To quantify the core temperature gain derived from inhalation rewarming, 10 subjects were immersed in seawater (mean temperature 12 degrees C) until a 2 degree C drop in rectal temperature occurred, and were then rewarmed by breathing hot saturated air at 45 degrees C for 30 min. Each subject was rewarmed once breathing air and once rebreathing a controlled fraction of expired air adjusted to produce a hyperventilation of 50 l/min. After 30 min of rewarming mean rectal temperature had increased 0.39 degrees C in subjects breathing air compared with 0.77 degrees C in those hyperventilating (P less than 0.01). Corresponding gains in tympanic temperatures were 1.1 and 1.5 degrees C, respectively. Calculations indicate that the additional heat input with hyperventilation yielded a core (rectal) temperature gain of 5.1 X 10(-4) degrees C/l. It is concluded that each additional 10 l/min of ventilation of hot saturated air will increase the rate of core rewarming from hypothermia by approximately 0.3 degrees C/h.", "contents": "Thermal increment provided by inhalation rewarming from hypothermia. To quantify the core temperature gain derived from inhalation rewarming, 10 subjects were immersed in seawater (mean temperature 12 degrees C) until a 2 degree C drop in rectal temperature occurred, and were then rewarmed by breathing hot saturated air at 45 degrees C for 30 min. Each subject was rewarmed once breathing air and once rebreathing a controlled fraction of expired air adjusted to produce a hyperventilation of 50 l/min. After 30 min of rewarming mean rectal temperature had increased 0.39 degrees C in subjects breathing air compared with 0.77 degrees C in those hyperventilating (P less than 0.01). Corresponding gains in tympanic temperatures were 1.1 and 1.5 degrees C, respectively. Calculations indicate that the additional heat input with hyperventilation yielded a core (rectal) temperature gain of 5.1 X 10(-4) degrees C/l. It is concluded that each additional 10 l/min of ventilation of hot saturated air will increase the rate of core rewarming from hypothermia by approximately 0.3 degrees C/h."}
{"id": "PMID:468625", "title": "Metabolic and cardiopulmonary responses to wheelchair and bicycle ergometry.", "content": "To evaluate wheelchair activity in reference to a more familiar mode of locomotion, metabolic and cardiopulmonary responses to wheelchair ergometer (WERG) and bicycle ergometer (BERG) exercise were compared. Eighteen able-bodies subjects were tested on a combination wheelchair-bicycle ergometer. Oxygen uptake (VO2), respiratory exchange ratio (R), pulmonary ventilation (VE), ventilatory equivalent (VE/VO2), percent net mechanical efficiency (ME), and heart rate (HR) were determined at power output (PO) levels of 30, 90, and 150 kpm/min on each ergometer. For WERG and BERG exercise, VO2, VE, and HR increased linearly with PO. Generally, VO2, R, VE, VE/VO2, and HR responses were higher (P less than 0.05) during WERG than BERG exercise at each PO. Blood lactate was determined after 150 kpm/min, and found to be higher (P less than 0.05) during WERG than BERG exercise. ME increased with PO and was lower (P less than 0.05) for WERG than BERG exercise at each PO level. The greater metabolic and cardiopulmonary responses observed during WERG exercise may be due to inefficient biomechanics and the relatively small upper body musculature used for propulsion.", "contents": "Metabolic and cardiopulmonary responses to wheelchair and bicycle ergometry. To evaluate wheelchair activity in reference to a more familiar mode of locomotion, metabolic and cardiopulmonary responses to wheelchair ergometer (WERG) and bicycle ergometer (BERG) exercise were compared. Eighteen able-bodies subjects were tested on a combination wheelchair-bicycle ergometer. Oxygen uptake (VO2), respiratory exchange ratio (R), pulmonary ventilation (VE), ventilatory equivalent (VE/VO2), percent net mechanical efficiency (ME), and heart rate (HR) were determined at power output (PO) levels of 30, 90, and 150 kpm/min on each ergometer. For WERG and BERG exercise, VO2, VE, and HR increased linearly with PO. Generally, VO2, R, VE, VE/VO2, and HR responses were higher (P less than 0.05) during WERG than BERG exercise at each PO. Blood lactate was determined after 150 kpm/min, and found to be higher (P less than 0.05) during WERG than BERG exercise. ME increased with PO and was lower (P less than 0.05) for WERG than BERG exercise at each PO level. The greater metabolic and cardiopulmonary responses observed during WERG exercise may be due to inefficient biomechanics and the relatively small upper body musculature used for propulsion."}
{"id": "PMID:468626", "title": "Rib cage deformation during static inspiratory efforts.", "content": "Patterns of rib cage (RC) deformation were studied in six normal subjects during moderate static inspiratory efforts such that esophageal pressure (Pes) as an index of transthoracic pressure fell to between -30 and -60 cmH2O during each maneuver. At lung volumes below 50% inspiratory capacity (IC), static inspiratory efforts deformed RC to a more elliptical shape; RC lateral diameter became smaller and RC lateral diameter became larger. However, at high lung volumes (greater than 50% IC) the opposite change in RC dimensions occurred despite similar changes in Pes, i.e., the RC became more circular. These differences in RC deformation did not appear to be a possive consequence of increased lung volume because the RC could be voluntarily deformed to a more circular shape at low lung volume when a) subjects performed static inspiratory efforts mainly with their intercostal and accessory muscles rather than their diaphragm as judged by a smaller change in transdiaphragmatic pressure for the same Pes; or b) subjects statically contracted their diaphragm with it held in a relatively flattened configuration as assessed by a large abdominal AP dimension. We suggest that deformation of the RC during static inspiratory efforts is not as predictable as has previously been suggested but depends on the pattern of contraction and configuration of the respiratory muscles.", "contents": "Rib cage deformation during static inspiratory efforts. Patterns of rib cage (RC) deformation were studied in six normal subjects during moderate static inspiratory efforts such that esophageal pressure (Pes) as an index of transthoracic pressure fell to between -30 and -60 cmH2O during each maneuver. At lung volumes below 50% inspiratory capacity (IC), static inspiratory efforts deformed RC to a more elliptical shape; RC lateral diameter became smaller and RC lateral diameter became larger. However, at high lung volumes (greater than 50% IC) the opposite change in RC dimensions occurred despite similar changes in Pes, i.e., the RC became more circular. These differences in RC deformation did not appear to be a possive consequence of increased lung volume because the RC could be voluntarily deformed to a more circular shape at low lung volume when a) subjects performed static inspiratory efforts mainly with their intercostal and accessory muscles rather than their diaphragm as judged by a smaller change in transdiaphragmatic pressure for the same Pes; or b) subjects statically contracted their diaphragm with it held in a relatively flattened configuration as assessed by a large abdominal AP dimension. We suggest that deformation of the RC during static inspiratory efforts is not as predictable as has previously been suggested but depends on the pattern of contraction and configuration of the respiratory muscles."}
{"id": "PMID:468627", "title": "Breathing pattern and ventilatory response to carbon dioxide in divers.", "content": "The breathing pattern and ventilatory response to carbon dioxide of 10 experienced divers was compared with that of 10 nondivers of similar age and build. Breathing pattern was described by the equation VE = M (VT - K) and the response to carbon dioxide by VE = S(PCO2 - B). The divers exhibited a value form 27% lower than the nondivers; S was 33% lower. The difference was significant (P less than 0.05) in both cases. B was significantly higher (P less than 0.05) in the divers than nondivers. These differences are not attributable to age, build, or vital capacity. S was well correlated with M when all subjects were considered a single group. Within the diving group no correlation of S and M with diving experience was found.", "contents": "Breathing pattern and ventilatory response to carbon dioxide in divers. The breathing pattern and ventilatory response to carbon dioxide of 10 experienced divers was compared with that of 10 nondivers of similar age and build. Breathing pattern was described by the equation VE = M (VT - K) and the response to carbon dioxide by VE = S(PCO2 - B). The divers exhibited a value form 27% lower than the nondivers; S was 33% lower. The difference was significant (P less than 0.05) in both cases. B was significantly higher (P less than 0.05) in the divers than nondivers. These differences are not attributable to age, build, or vital capacity. S was well correlated with M when all subjects were considered a single group. Within the diving group no correlation of S and M with diving experience was found."}
{"id": "PMID:468628", "title": "Thermoregulation in swimmers and runners.", "content": "Thermoregulatory responses of six trained swimmers and five runners to cold and heat were evaluated during 30 min of exercise (60% VO2max) while immersed to the neck in 20, 25, 30, and 35 degrees C water. Mean oxygen uptake was similar for both groups during all four trials. Changes in metabolic rate during the 8th to 28th min were significantly greater for the runners in 20 degrees C water, and swimmers in 30 and 35 degrees C water. Heart rates, Tsk, delta Tre, Tb, body heat content, and heat storage were dependent on water temperature. Runners were able to attain higher sweat rates than swimmers in 35 degrees C water. Swimmers had significantly greater tissue conductance values in the 35 degrees C exposure. Swimmers thermoregulated better in 20 degrees C water than runners, possibly due to a larger surface area-to-volume ratio, percentage body fat, subcutaneous fat, or improved vasomotor control. Exercise in the heat was better tolerated by runners. Physical training in water does not improve heat acclimatization to the extent of training in air, but does improve cold tolerance.", "contents": "Thermoregulation in swimmers and runners. Thermoregulatory responses of six trained swimmers and five runners to cold and heat were evaluated during 30 min of exercise (60% VO2max) while immersed to the neck in 20, 25, 30, and 35 degrees C water. Mean oxygen uptake was similar for both groups during all four trials. Changes in metabolic rate during the 8th to 28th min were significantly greater for the runners in 20 degrees C water, and swimmers in 30 and 35 degrees C water. Heart rates, Tsk, delta Tre, Tb, body heat content, and heat storage were dependent on water temperature. Runners were able to attain higher sweat rates than swimmers in 35 degrees C water. Swimmers had significantly greater tissue conductance values in the 35 degrees C exposure. Swimmers thermoregulated better in 20 degrees C water than runners, possibly due to a larger surface area-to-volume ratio, percentage body fat, subcutaneous fat, or improved vasomotor control. Exercise in the heat was better tolerated by runners. Physical training in water does not improve heat acclimatization to the extent of training in air, but does improve cold tolerance."}
{"id": "PMID:468629", "title": "Effects of training on postexercise limb muscle EMG synchronous to respiration.", "content": "Twenty university students (10 trained and 10 untrained) participated in 60 sessions testing the effect of training on postexercise electromyogram synchronous to respiration. The latter was recorded for m. biceps brachii dextra, m. quadriceps femoris dextra, and m. gastrocnemium dextra. The respiration rate was taken before and after three levels of functional effort performed on a bicycle ergometer. The first (2 X 30 s with a 20-s interval at 100 rpm and 245 W) and the second (5 X 1 min with a 30-s interval after each min, at 90 rpm and 200 W) were of speed-developing nature; the third (20 min at 60 rpm and 117 W with 1-min interval every 5 min) was to develop endurance. Our observations show that the greatest percentage of a synchronous-to-respiration muscle bioelectric activity can be observed after the first two types of effort. In the third type the percentage is smaller and the duration of the activity is shorter. On the other hand, the percentage of biopotential occurrence is greater for untrained persons, with a considerable longer disappearance time. The phenomenon can be used as a criterion in the athlete's functional diagnosis.", "contents": "Effects of training on postexercise limb muscle EMG synchronous to respiration. Twenty university students (10 trained and 10 untrained) participated in 60 sessions testing the effect of training on postexercise electromyogram synchronous to respiration. The latter was recorded for m. biceps brachii dextra, m. quadriceps femoris dextra, and m. gastrocnemium dextra. The respiration rate was taken before and after three levels of functional effort performed on a bicycle ergometer. The first (2 X 30 s with a 20-s interval at 100 rpm and 245 W) and the second (5 X 1 min with a 30-s interval after each min, at 90 rpm and 200 W) were of speed-developing nature; the third (20 min at 60 rpm and 117 W with 1-min interval every 5 min) was to develop endurance. Our observations show that the greatest percentage of a synchronous-to-respiration muscle bioelectric activity can be observed after the first two types of effort. In the third type the percentage is smaller and the duration of the activity is shorter. On the other hand, the percentage of biopotential occurrence is greater for untrained persons, with a considerable longer disappearance time. The phenomenon can be used as a criterion in the athlete's functional diagnosis."}
{"id": "PMID:468630", "title": "Comparison of phrenic motoneuron responses to hypercapnia and isocapnic hypoxia.", "content": "This study was undertaken to compare phrenic motoneuron responses to hypercapnia and isocapnic hypoxia. Efferent activity of single phrenic nerve fibers was recorded with that of the contralateral phrenic nerve in decerebrate cats which were vagotomized, paralyzed, and artificially ventilated. At normocapnia in hyperoxia, single phrenic fibers were distributed into approximately equal \"early\" and \"late\" populations according to their onset of activity relative to the period of the phrenic burst. Elevations of PACO2 or diminutions of PAO2 resulted in progressive increases in the number of spikes per respiratory cycle and decreases in the modal interspike interval for both early and late units. Moreover, either stimulus caused an onset of late unit activity at progressively earlier portions of inspiration. At equivalent levels of peak integrated phrenic discharge achieved at normocapnia or hypercapnia as compared to normoxia or hypoxia, there were no differences in activity patterns for either early or late units. It is concluded that hypoxia-induced alterations in the activity of single phrenic motoneurons are identical to those changes resulting from hypercapnia.", "contents": "Comparison of phrenic motoneuron responses to hypercapnia and isocapnic hypoxia. This study was undertaken to compare phrenic motoneuron responses to hypercapnia and isocapnic hypoxia. Efferent activity of single phrenic nerve fibers was recorded with that of the contralateral phrenic nerve in decerebrate cats which were vagotomized, paralyzed, and artificially ventilated. At normocapnia in hyperoxia, single phrenic fibers were distributed into approximately equal \"early\" and \"late\" populations according to their onset of activity relative to the period of the phrenic burst. Elevations of PACO2 or diminutions of PAO2 resulted in progressive increases in the number of spikes per respiratory cycle and decreases in the modal interspike interval for both early and late units. Moreover, either stimulus caused an onset of late unit activity at progressively earlier portions of inspiration. At equivalent levels of peak integrated phrenic discharge achieved at normocapnia or hypercapnia as compared to normoxia or hypoxia, there were no differences in activity patterns for either early or late units. It is concluded that hypoxia-induced alterations in the activity of single phrenic motoneurons are identical to those changes resulting from hypercapnia."}
{"id": "PMID:468631", "title": "Effects of inspiratory flow pattern on gas exchange in normal and abnormal lungs.", "content": "To study the influence of inspiratory flow pattern on gas exchange in normal and abnormal lungs, 15 dogs were mechanically ventilated with the ascending or descending half of a rectified sine-wave flow pattern during pentobarbital anesthesia. Blood-gas status and cardiac output were monitored during each pattern. Pulmonary injury was induced in 9 animals by prior injection of oleic acid into the right atrium. Ventilation was with room air and zero end-expiratory pressure. Although no significant difference was seen between the patterns in animals with normal lung function (PaO2 greater than 85 Torr on the ascending pattern), the descending pattern resulted in a rise in PaO2 of about 10% (P less than 0.01) in animals with abnormal lung function (PaO2 less than 70 Torr on the ascending pattern). No significant difference in PaCO2 or cardiac output was detected when flow pattern was changed in any of the animals. We conclude that inspiratory flow pattern is not a significant factor when ventilating normal lungs, but may improve gas exchange significantly when severe ventilation-perfusion maldistributions exist.", "contents": "Effects of inspiratory flow pattern on gas exchange in normal and abnormal lungs. To study the influence of inspiratory flow pattern on gas exchange in normal and abnormal lungs, 15 dogs were mechanically ventilated with the ascending or descending half of a rectified sine-wave flow pattern during pentobarbital anesthesia. Blood-gas status and cardiac output were monitored during each pattern. Pulmonary injury was induced in 9 animals by prior injection of oleic acid into the right atrium. Ventilation was with room air and zero end-expiratory pressure. Although no significant difference was seen between the patterns in animals with normal lung function (PaO2 greater than 85 Torr on the ascending pattern), the descending pattern resulted in a rise in PaO2 of about 10% (P less than 0.01) in animals with abnormal lung function (PaO2 less than 70 Torr on the ascending pattern). No significant difference in PaCO2 or cardiac output was detected when flow pattern was changed in any of the animals. We conclude that inspiratory flow pattern is not a significant factor when ventilating normal lungs, but may improve gas exchange significantly when severe ventilation-perfusion maldistributions exist."}
{"id": "PMID:468632", "title": "Mechanism of rapid, shallow breathing after ozone exposure in conscious dogs.", "content": "In 10 experiments on 3 conscious dogs exercising on a treadmill, we studied the effect of ozone on base-line ventilation and on ventilatory responses to inhaled bronchoconstrictor drugs. Prior to ozone exposure, inhalation of histamine diphosphate aerosol (1%; 5 breaths) increased respiratory frequency (f) by 86 +/- 11% (mean +/- SE), and inhalation of prostaglandin F2 alpha (PGF2 alpha) aerosol (0.1%; 5 breaths) increased f by 74 +/- 16%. Immediately after ozone exposure %0.65 ppm; 2 h), steady-state base line f was increased by 120 +/- 18% and tidal volume (VT) was decreased by 43 +/- 5%. When conduction in the cervical vagus nerves (that were exteriorized permanently in skin loops) was blocked by cooling, these changes caused by ozone were abolished (P greater than 0.05). The increased responses to both histamine and PGF2 alpha aerosols after ozone were unaffected by pretreatment of isoproterenol aerosol (0.5%; 15 breaths), but were completely abolished by vagal cooling. Our studies indicate that ozone-induced rapid, shallow breathing and the increased ventilatory responses to inhaled histamine and PGF2 alpha aerosols are mediated through vagal afferent pathways.", "contents": "Mechanism of rapid, shallow breathing after ozone exposure in conscious dogs. In 10 experiments on 3 conscious dogs exercising on a treadmill, we studied the effect of ozone on base-line ventilation and on ventilatory responses to inhaled bronchoconstrictor drugs. Prior to ozone exposure, inhalation of histamine diphosphate aerosol (1%; 5 breaths) increased respiratory frequency (f) by 86 +/- 11% (mean +/- SE), and inhalation of prostaglandin F2 alpha (PGF2 alpha) aerosol (0.1%; 5 breaths) increased f by 74 +/- 16%. Immediately after ozone exposure %0.65 ppm; 2 h), steady-state base line f was increased by 120 +/- 18% and tidal volume (VT) was decreased by 43 +/- 5%. When conduction in the cervical vagus nerves (that were exteriorized permanently in skin loops) was blocked by cooling, these changes caused by ozone were abolished (P greater than 0.05). The increased responses to both histamine and PGF2 alpha aerosols after ozone were unaffected by pretreatment of isoproterenol aerosol (0.5%; 15 breaths), but were completely abolished by vagal cooling. Our studies indicate that ozone-induced rapid, shallow breathing and the increased ventilatory responses to inhaled histamine and PGF2 alpha aerosols are mediated through vagal afferent pathways."}
{"id": "PMID:468633", "title": "Effect of hyperoxia on oxygen consumption in exercising ponies.", "content": "Published reports of oxygen consumption (VO2) during exercise in hyperoxia are equivocal. By and large, when measured at the lung using respiratory gas equations, VO2 is elevated in hyperoxia and, when measured at the blood-tissue level using the cardiovascular Fick (CVF) equation, it is unchanged. We sought to provide some insight into this problem by making through the use of both equations simultaneous determinations of VO2 during hyperoxia in exercising ponies. In normoxia, during treadmill exercise (115 m/min, 10% grade) of seven ponies, there was no difference in exercise VO2, whether it was measured by the Haldane transformation (HT) or CVF equations (P greater than 0.05). In hyperoxia, the exercise VO2 was significantly increased from the normoxia condition (P less than 0.05) when measured by the HT equation but not when measured by the CVF equation (P greater than 0.05). By use of the CVF equation as the method of choice for VO2 determinations in hyperoxia, the present data show no change in exercise VO2 in the hyperoxic condition.", "contents": "Effect of hyperoxia on oxygen consumption in exercising ponies. Published reports of oxygen consumption (VO2) during exercise in hyperoxia are equivocal. By and large, when measured at the lung using respiratory gas equations, VO2 is elevated in hyperoxia and, when measured at the blood-tissue level using the cardiovascular Fick (CVF) equation, it is unchanged. We sought to provide some insight into this problem by making through the use of both equations simultaneous determinations of VO2 during hyperoxia in exercising ponies. In normoxia, during treadmill exercise (115 m/min, 10% grade) of seven ponies, there was no difference in exercise VO2, whether it was measured by the Haldane transformation (HT) or CVF equations (P greater than 0.05). In hyperoxia, the exercise VO2 was significantly increased from the normoxia condition (P less than 0.05) when measured by the HT equation but not when measured by the CVF equation (P greater than 0.05). By use of the CVF equation as the method of choice for VO2 determinations in hyperoxia, the present data show no change in exercise VO2 in the hyperoxic condition."}
{"id": "PMID:468634", "title": "Acceleration forces at eye level experienced with rotation on the horizontal bar.", "content": "Negative acceleration forces (-Gz) experienced at eye level have been associated with preretinal hemorrhage and headache. These signs and symptoms were found in individuals who experienced negative (toward the head) force while rotating on a horizontal bar or hanging from a trapeze. Lightweight accelerometers were used to measure -Gz experienced at eye level in children and adult gymnasts performing a single-knee backswing on a horizontal bar. Rate of onset of -Gz, peak -Gz, time experiencing -Gz, area of curve (G.second), and mean force (area/time) were calculated. There was no significant difference between the children and the adult gymnasts in any of the above parameters. The best gymnast had a maximum rate of onset of 38.15 G/s and the maximum negative force experienced was 5.52 G. The maximum rate of onset for a child was 41.56 G/s and the maximum negative force experienced was 5.73 G. Compared with -Gz tolerance curves generated on a centrifuge the best gymnast would have become symptomatic while performing this maneuver in 6 s. The best child would have become symptomatic in 25 s. These tolerance limits can be easily exceeded by gymnasts and by the monkey-bar enthusiast.", "contents": "Acceleration forces at eye level experienced with rotation on the horizontal bar. Negative acceleration forces (-Gz) experienced at eye level have been associated with preretinal hemorrhage and headache. These signs and symptoms were found in individuals who experienced negative (toward the head) force while rotating on a horizontal bar or hanging from a trapeze. Lightweight accelerometers were used to measure -Gz experienced at eye level in children and adult gymnasts performing a single-knee backswing on a horizontal bar. Rate of onset of -Gz, peak -Gz, time experiencing -Gz, area of curve (G.second), and mean force (area/time) were calculated. There was no significant difference between the children and the adult gymnasts in any of the above parameters. The best gymnast had a maximum rate of onset of 38.15 G/s and the maximum negative force experienced was 5.52 G. The maximum rate of onset for a child was 41.56 G/s and the maximum negative force experienced was 5.73 G. Compared with -Gz tolerance curves generated on a centrifuge the best gymnast would have become symptomatic while performing this maneuver in 6 s. The best child would have become symptomatic in 25 s. These tolerance limits can be easily exceeded by gymnasts and by the monkey-bar enthusiast."}
{"id": "PMID:468635", "title": "Breath-by-breath variation of FRC: effect on VO2 and VCO2 measured at the mouth.", "content": "We examined breath-by-breath (B-B) variations of FRC (delta FRC) and their effect on measured O2 and CO2 gas exchange in 52 2- to 4-min segments of continuous air breathing obtained in 29 patients (age range 6--50 yr). Respiratory frequency ranged from 13 to 43 breaths/min, VE from 6.7 to 22.5 l/min (BTPS), and expired VT from 234 to 1,370 ml (BTPS). Computer analysis was based on the following source data measured at the mouth: inspired (VI) and expired (VE) gas flow, FN2, FO2 and FCO2. The analysis provides B-B evaluation of VI, VE, delta FRC in terms of VN2, and VO2 and VCO2 at the mouth and at the alveolar level, i.e., after correction for delta FRC. Significant B-B variations of FRC were found in all studies. delta FRC ranged from +360 to -360 ml (BTPS). For single respiratory cycles VI - VE is primarily a function of N2 exchange at the mouth (VMN2). VO2 and VCO2, uncorrected for delta FRC, are significantly more dispersed about mean values than the corrected gas uptakes (P less than 0.0005). The data support the view that the assumption of VIN2 = VEN2 is invalid for single respiratory cycles. Determination of breath-by-breath VO2 and VCO2 should therefore, not be based on steady-state gas uptake equations. It requires measurement of both inspired and expired breath volumes and evaluation of N2 gas exchange.", "contents": "Breath-by-breath variation of FRC: effect on VO2 and VCO2 measured at the mouth. We examined breath-by-breath (B-B) variations of FRC (delta FRC) and their effect on measured O2 and CO2 gas exchange in 52 2- to 4-min segments of continuous air breathing obtained in 29 patients (age range 6--50 yr). Respiratory frequency ranged from 13 to 43 breaths/min, VE from 6.7 to 22.5 l/min (BTPS), and expired VT from 234 to 1,370 ml (BTPS). Computer analysis was based on the following source data measured at the mouth: inspired (VI) and expired (VE) gas flow, FN2, FO2 and FCO2. The analysis provides B-B evaluation of VI, VE, delta FRC in terms of VN2, and VO2 and VCO2 at the mouth and at the alveolar level, i.e., after correction for delta FRC. Significant B-B variations of FRC were found in all studies. delta FRC ranged from +360 to -360 ml (BTPS). For single respiratory cycles VI - VE is primarily a function of N2 exchange at the mouth (VMN2). VO2 and VCO2, uncorrected for delta FRC, are significantly more dispersed about mean values than the corrected gas uptakes (P less than 0.0005). The data support the view that the assumption of VIN2 = VEN2 is invalid for single respiratory cycles. Determination of breath-by-breath VO2 and VCO2 should therefore, not be based on steady-state gas uptake equations. It requires measurement of both inspired and expired breath volumes and evaluation of N2 gas exchange."}
{"id": "PMID:468636", "title": "Effects of carotid body denervation on respiratory pattern of awake cats.", "content": "Eight awake cats have been studied before and after carotid denervation during air and oxygen breathing, and during hypercapnia. Analysis of the variables that characterize the spirogram shows that carotid denervation consistently results in a decrease of the mean inspiratory flow (VT/TI), causing a decrease in tidal volume (VT) and ventilation with a relative alveolar hypercapnia. In carotid-denervated animals, inhalation of oxygen results in an increase in ventilation due to an augmentation of VT/TI and VT and a relative hypocapnia. TI does not significantly change in the different conditions whereas TE is significantly affected. TE seems therefore to be more closely related to the rate of rise of inspiratory activity than to inspiratory duration.", "contents": "Effects of carotid body denervation on respiratory pattern of awake cats. Eight awake cats have been studied before and after carotid denervation during air and oxygen breathing, and during hypercapnia. Analysis of the variables that characterize the spirogram shows that carotid denervation consistently results in a decrease of the mean inspiratory flow (VT/TI), causing a decrease in tidal volume (VT) and ventilation with a relative alveolar hypercapnia. In carotid-denervated animals, inhalation of oxygen results in an increase in ventilation due to an augmentation of VT/TI and VT and a relative hypocapnia. TI does not significantly change in the different conditions whereas TE is significantly affected. TE seems therefore to be more closely related to the rate of rise of inspiratory activity than to inspiratory duration."}
{"id": "PMID:468637", "title": "One- and three-minute exercise response in coronary artery disease.", "content": "Treadmill exercise responses of oxygen uptake (VO2), CO2 production (VCO2), and venoarterial CO2 difference (Cv-Ca), measured by CO2 rebreathing (Defares technique) at 1 and 3 min of exercise, were studied in two separate series of control (apparently healthy) and coronary artery disease (CAD) subjects. In the 1-min series there were 21 controls and 15 CAD subjects. In the 3-min series there were 21 controls and 18 CAD subjects. All were males, ages 30--60 yr, and the CAD subjects had histories of myocardial infarction. In both series VO2 at 1 min and power requirement (P ) = 1,000 (VO2-1-1,000) was estimated where P =weight (kg) X grade (fractional) X walking speed (m/min). In the 1-min series (P = 1,000) all measurements were made at 45--60 s in separate test runs. In the 3-min series P was 750 kg.m/min, VCO2 and VO2 were measured at 2.5--3 min exercise, and Cv-Ca was measured at 3--3.25 min in the same test run. Data indicate that the average cardiac output (Q) for control subjects was 15 l/min in both series with lower values for CAD subjects. At 1 min reduced Q was accompanied by reduced VO2 or VCO2 and increased Cv-Ca, whereas at 3 min reduced Q was accompanied by increased Cv-Ca.", "contents": "One- and three-minute exercise response in coronary artery disease. Treadmill exercise responses of oxygen uptake (VO2), CO2 production (VCO2), and venoarterial CO2 difference (Cv-Ca), measured by CO2 rebreathing (Defares technique) at 1 and 3 min of exercise, were studied in two separate series of control (apparently healthy) and coronary artery disease (CAD) subjects. In the 1-min series there were 21 controls and 15 CAD subjects. In the 3-min series there were 21 controls and 18 CAD subjects. All were males, ages 30--60 yr, and the CAD subjects had histories of myocardial infarction. In both series VO2 at 1 min and power requirement (P ) = 1,000 (VO2-1-1,000) was estimated where P =weight (kg) X grade (fractional) X walking speed (m/min). In the 1-min series (P = 1,000) all measurements were made at 45--60 s in separate test runs. In the 3-min series P was 750 kg.m/min, VCO2 and VO2 were measured at 2.5--3 min exercise, and Cv-Ca was measured at 3--3.25 min in the same test run. Data indicate that the average cardiac output (Q) for control subjects was 15 l/min in both series with lower values for CAD subjects. At 1 min reduced Q was accompanied by reduced VO2 or VCO2 and increased Cv-Ca, whereas at 3 min reduced Q was accompanied by increased Cv-Ca."}
{"id": "PMID:468638", "title": "Respiratory response to HCl acidosis in dogs after carotid body denervation.", "content": "To test the participation of the carotid bodies in the respiratory response to metabolic acidosis, six dogs were infused with HCl for 2 h followed by HCl feeding to prolong the acidosis to 48 h. This protocol was repeated after carotid body denervation (CBD). Mean control PCO2 rose by 7.3 Torr after CBD. PCO2 fell comparably during acidosis before and after CBD at all time periods from 30 to 48 h and returned to control levels by 72 h. The pH ranged from 7.10 to 7.30 during acidosis pre- and post-CBD. The decreases in pH and bicarbonate concentration did not differ significantly at any time interval between the pre- and post-CBD studies. This study indicates that in dogs moderately severe HCl acidosis stimulates ventilation acutely and chronically through a central mechanism in the absence of the carotid bodies.", "contents": "Respiratory response to HCl acidosis in dogs after carotid body denervation. To test the participation of the carotid bodies in the respiratory response to metabolic acidosis, six dogs were infused with HCl for 2 h followed by HCl feeding to prolong the acidosis to 48 h. This protocol was repeated after carotid body denervation (CBD). Mean control PCO2 rose by 7.3 Torr after CBD. PCO2 fell comparably during acidosis before and after CBD at all time periods from 30 to 48 h and returned to control levels by 72 h. The pH ranged from 7.10 to 7.30 during acidosis pre- and post-CBD. The decreases in pH and bicarbonate concentration did not differ significantly at any time interval between the pre- and post-CBD studies. This study indicates that in dogs moderately severe HCl acidosis stimulates ventilation acutely and chronically through a central mechanism in the absence of the carotid bodies."}
{"id": "PMID:468639", "title": "Abolition of a viscerosomatic reflex during oxygen deprivation.", "content": "Effect of hypoxia was studied on the J reflex, a term used for the reflex inhibition of muscular exercise by activation of type J pulmonary endings. Hypoxia was induced by ventilating the animal with gas mixtures varying from 18 to 9% oxygen in nitrogen. The blood gas tensions of the arterial blood (PaO2) were measured before, during, and after hypoxia. It was found that the J reflex is sensitive to a reduction in oxygen. A fall in PaO2 between 67 and 47 Torr abolished this reflex. Studies were also undertaken to exclude the possibility that the effect of hypoxia was predominantly exerted on monosynaptic reflex itself. A similar fall in PaO2 also depressed the monosynaptic reflex. Minimum time required for the significant depression was 32 s. On the contrary the J reflex was abolished within this period suggesting that the abolition of J reflex is independent of the changes in monosynaptic reflex during hypoxia.", "contents": "Abolition of a viscerosomatic reflex during oxygen deprivation. Effect of hypoxia was studied on the J reflex, a term used for the reflex inhibition of muscular exercise by activation of type J pulmonary endings. Hypoxia was induced by ventilating the animal with gas mixtures varying from 18 to 9% oxygen in nitrogen. The blood gas tensions of the arterial blood (PaO2) were measured before, during, and after hypoxia. It was found that the J reflex is sensitive to a reduction in oxygen. A fall in PaO2 between 67 and 47 Torr abolished this reflex. Studies were also undertaken to exclude the possibility that the effect of hypoxia was predominantly exerted on monosynaptic reflex itself. A similar fall in PaO2 also depressed the monosynaptic reflex. Minimum time required for the significant depression was 32 s. On the contrary the J reflex was abolished within this period suggesting that the abolition of J reflex is independent of the changes in monosynaptic reflex during hypoxia."}
{"id": "PMID:468641", "title": "Cerebral gas exchange: perfusion and diffusion limitations.", "content": "The simultaneous washout of argon and methane from brain tissue stores was studied in anesthetized dogs that were rendered either hypocapnic or hypercapnic. The washout pattern can be analyzed in terms of two compartments with different rate constants. The two tracers were eliminated at the same rate from any one compartment in either experimental condition. Because argon and methane have the same blood-brain partition coefficient, and a ratio of molecular weights of 2.5, we conclude that their transport into or from the brain is not limited by diffusion over a wide range of cerebral blood flows.", "contents": "Cerebral gas exchange: perfusion and diffusion limitations. The simultaneous washout of argon and methane from brain tissue stores was studied in anesthetized dogs that were rendered either hypocapnic or hypercapnic. The washout pattern can be analyzed in terms of two compartments with different rate constants. The two tracers were eliminated at the same rate from any one compartment in either experimental condition. Because argon and methane have the same blood-brain partition coefficient, and a ratio of molecular weights of 2.5, we conclude that their transport into or from the brain is not limited by diffusion over a wide range of cerebral blood flows."}
{"id": "PMID:468642", "title": "Hypopnea consequent to reduced pulmonary blood flow in the dog.", "content": "The ventilatory responses to diminished pulmonary blood flow (Qc), as a result of partial cardiopulmonary bypass (PCB), were studied in chloralose-urethan-anesthetized dogs. Qc was reduced by diverting vena caval blood through a membrane gas exchanger and returning it to the ascending aorta. PCB flows of 400--1,600 ml/min were utilized for durations of 2--3 min. Decreasing Qc, while maintaining systemic arterial blood gases and perfusion, results in a significant (P less than 0.05) decrease in expiratory ventilation (VE) (15.9%) and alveolar ventilation (VA) (31.0%). The ventilatory decreases demonstrated for this intact group persist after bilateral cervical vagotomy (Vx), carotid body and carotid sinus denervation (Cx), and combined Vx and Cx. The changes in VE and VA were significantly (P less than 0.001) correlated with VCO2 changes, r = 0.80 and r = 0.93, respectively. These ventilatory changes were associated with an overall average decrease in left ventricular PCO2 of 2.1 Torr; this decrease was significant (P less than 0.05) only in the intact and Cx groups. Decreasing pulmonary blood flow results in a decrease in ventilation that may be CO2 related; however, the exact mechanism remains obscure but must have a component that is independent of vagally mediated cardiac and pulmonary afferents and peripheral baroreceptor and chemoreceptor afferents.", "contents": "Hypopnea consequent to reduced pulmonary blood flow in the dog. The ventilatory responses to diminished pulmonary blood flow (Qc), as a result of partial cardiopulmonary bypass (PCB), were studied in chloralose-urethan-anesthetized dogs. Qc was reduced by diverting vena caval blood through a membrane gas exchanger and returning it to the ascending aorta. PCB flows of 400--1,600 ml/min were utilized for durations of 2--3 min. Decreasing Qc, while maintaining systemic arterial blood gases and perfusion, results in a significant (P less than 0.05) decrease in expiratory ventilation (VE) (15.9%) and alveolar ventilation (VA) (31.0%). The ventilatory decreases demonstrated for this intact group persist after bilateral cervical vagotomy (Vx), carotid body and carotid sinus denervation (Cx), and combined Vx and Cx. The changes in VE and VA were significantly (P less than 0.001) correlated with VCO2 changes, r = 0.80 and r = 0.93, respectively. These ventilatory changes were associated with an overall average decrease in left ventricular PCO2 of 2.1 Torr; this decrease was significant (P less than 0.05) only in the intact and Cx groups. Decreasing pulmonary blood flow results in a decrease in ventilation that may be CO2 related; however, the exact mechanism remains obscure but must have a component that is independent of vagally mediated cardiac and pulmonary afferents and peripheral baroreceptor and chemoreceptor afferents."}
{"id": "PMID:468643", "title": "Real-time moment analysis of pulmonary nitrogen washout.", "content": "A real-time moment analysis was applied to multibreath nitrogen-washout dynamics of the lung. Because the analysis accounts for breathing pattern variations, subjects could perform the washout with spontaneous breathing. The real-time digital processing of the nitrogen and flow signals incorporates filtering, delay compensation, and corrections for the effects of gas composition and temperature changes. In our study, moment analysis of the multibreath washout was evaluated for 37 subjects: 6 normal nonsmokers, 4 \"normal\" smokers, 6 asthmatics, 12 with diffuse interstitial disease, and 9 with chronic obstructive pulmonary disease. Moments were computed from end-tidal and breath-averaged nitrogen fractions. The ratio of 1st-to-0th moments was found to yield distinctions among subjects with different degrees of ventilation inhomogeneity, even between normal nonsmokers and \"normal\" smokers. With minimal computational facilities, this moment analysis not only provides a sensitive index of ventilatory dysfunction, but also a cost-effective tool.", "contents": "Real-time moment analysis of pulmonary nitrogen washout. A real-time moment analysis was applied to multibreath nitrogen-washout dynamics of the lung. Because the analysis accounts for breathing pattern variations, subjects could perform the washout with spontaneous breathing. The real-time digital processing of the nitrogen and flow signals incorporates filtering, delay compensation, and corrections for the effects of gas composition and temperature changes. In our study, moment analysis of the multibreath washout was evaluated for 37 subjects: 6 normal nonsmokers, 4 \"normal\" smokers, 6 asthmatics, 12 with diffuse interstitial disease, and 9 with chronic obstructive pulmonary disease. Moments were computed from end-tidal and breath-averaged nitrogen fractions. The ratio of 1st-to-0th moments was found to yield distinctions among subjects with different degrees of ventilation inhomogeneity, even between normal nonsmokers and \"normal\" smokers. With minimal computational facilities, this moment analysis not only provides a sensitive index of ventilatory dysfunction, but also a cost-effective tool."}
{"id": "PMID:468644", "title": "Transient response of the Geman-Miller respiratory oscillator model.", "content": "Recent evidence suggests that there is a persistence of elevated respiratory center activity for many respiratory cycles after the cessation of a neural stimulus. To explore the theoretical possibility that this behavior may be a consequence of the neural oscillator that dictates respiratory rhythm, the transient response of a mathematical model of a medullary respiratory oscillator recently described by Geman and Miller was examined using computer simulation. This concept was motivated by the presence of a persistent transient response behavior inherent in oscillators from mathematical physics. The results indicate that a transient persistence effect is evident in the model behavior under some conditions, and this effect as well as the steady-state amplitude is markedly sensitive to the shape of the saturation function that interconnects the neuron populations. Furthermore, this behavior is initiated by either an abrupt decrease in a tonic input or an abrupt decrease in the synaptic weights connecting subpopulations of neurons.", "contents": "Transient response of the Geman-Miller respiratory oscillator model. Recent evidence suggests that there is a persistence of elevated respiratory center activity for many respiratory cycles after the cessation of a neural stimulus. To explore the theoretical possibility that this behavior may be a consequence of the neural oscillator that dictates respiratory rhythm, the transient response of a mathematical model of a medullary respiratory oscillator recently described by Geman and Miller was examined using computer simulation. This concept was motivated by the presence of a persistent transient response behavior inherent in oscillators from mathematical physics. The results indicate that a transient persistence effect is evident in the model behavior under some conditions, and this effect as well as the steady-state amplitude is markedly sensitive to the shape of the saturation function that interconnects the neuron populations. Furthermore, this behavior is initiated by either an abrupt decrease in a tonic input or an abrupt decrease in the synaptic weights connecting subpopulations of neurons."}
{"id": "PMID:468645", "title": "A dryer for rapid response on-line expired gas measurements.", "content": "A dryer is described for use in on-line breath-by-breath gas analysis systems. The dryer continuously removes water vapor by condensation and controls the sample gas at 2 degrees C dew-point temperature or 5 Torr water vapor partial pressure. It is designed to operate at gas sampling flow rates from 0.5 to 1 1.min-1. The step-response time for the described system including a Beckman LB-2 CO2 analyzer, sampling tubing, and dryer is 120 ms at 1 l.min-1. The time required for gas samples to transport through the dryer is 105 ms at a gas sampling-flow rate of 1 l.min=1.", "contents": "A dryer for rapid response on-line expired gas measurements. A dryer is described for use in on-line breath-by-breath gas analysis systems. The dryer continuously removes water vapor by condensation and controls the sample gas at 2 degrees C dew-point temperature or 5 Torr water vapor partial pressure. It is designed to operate at gas sampling flow rates from 0.5 to 1 1.min-1. The step-response time for the described system including a Beckman LB-2 CO2 analyzer, sampling tubing, and dryer is 120 ms at 1 l.min-1. The time required for gas samples to transport through the dryer is 105 ms at a gas sampling-flow rate of 1 l.min=1."}
{"id": "PMID:468646", "title": "A method for measurement of oxygen uptake in neonates.", "content": "The frequent use of continuous positive airway pressure and positive end-expiratory pressure in newborn infants with pulmonary disease has prevented the use of conventional methods for measuring oxygen uptake (VO2) in intensive-care units. A feed-back replenishment technique for the determination of the oxygen uptake of these newborn infants has been developed. An instrument utilizing this method has been designed and built permitting continuous VO2 monitoring without interfering in the routine ventilatory therapy of the critically ill infant. Theoretical, bench, and animal experiments using room air as an inspired gas indicate instrument accuracies as a percentage of full scale of 2.4, 2.8, and 7.3, respectively. Preliminary trials on infants demonstrate that the instrument functions satisfactorily in the newborn intensive-care unit.", "contents": "A method for measurement of oxygen uptake in neonates. The frequent use of continuous positive airway pressure and positive end-expiratory pressure in newborn infants with pulmonary disease has prevented the use of conventional methods for measuring oxygen uptake (VO2) in intensive-care units. A feed-back replenishment technique for the determination of the oxygen uptake of these newborn infants has been developed. An instrument utilizing this method has been designed and built permitting continuous VO2 monitoring without interfering in the routine ventilatory therapy of the critically ill infant. Theoretical, bench, and animal experiments using room air as an inspired gas indicate instrument accuracies as a percentage of full scale of 2.4, 2.8, and 7.3, respectively. Preliminary trials on infants demonstrate that the instrument functions satisfactorily in the newborn intensive-care unit."}
{"id": "PMID:468647", "title": "Concurrent exercise of dogs using carousel-type treadmill.", "content": "To exercise several animals at the same time, a carousel-type treadmill was constructed. Horizontal extensions secured to a motorized central shaft allow six dogs to be run concurrently. To counter the effect of centrifugal force, a rubberized banked running track was built. The track was also designed so that it can be hosed down for easy maintenance. During the past 2 yr, the treadmill has not required any maintenance, and only minimal attention has been necessary on the track.", "contents": "Concurrent exercise of dogs using carousel-type treadmill. To exercise several animals at the same time, a carousel-type treadmill was constructed. Horizontal extensions secured to a motorized central shaft allow six dogs to be run concurrently. To counter the effect of centrifugal force, a rubberized banked running track was built. The track was also designed so that it can be hosed down for easy maintenance. During the past 2 yr, the treadmill has not required any maintenance, and only minimal attention has been necessary on the track."}
{"id": "PMID:468648", "title": "Temperature regulation and hypohydration: a singular view.", "content": "Body temperatures of exercising humans who have been denied water are elevated when compared to hydrated controls. The simplest \"explanation\" for the elevated temperature is a decrease in sensitivity of the sweating mechanism. This and similar \"explanations\" do not direct attention to basic causes but only the result(s) of more fundamental aspects of regulatory physiology. Among the items considered in this speculative presentation are influences of changes in osmolarity, specific ions, peptide hormones, fluid shifts, and muscular contractions during exercise. A hypothesis is offered for consideration in explaining elevations of body temperature in exercise with and without water replacement. In general, the hypothesis relates changes in hypothalamic osmotic pressure and/or ionic constituents with fluid and ionic events in muscle during exercise. The fluid and ionic shifts are probably proportional to the amount of lean body mass engaged in dynamic exercise. Since blood volume has also been shown to be related to lean body mass, similar relative work loads should lead to similar changes in the osmotic and/or ionic environment of the hypothalamus, thus resulting in similar increases in body temperature during exercise. Hypohydration is superimposed on this basic response. Increases in body temperature of resting hypohydrated subjects appear to be due to increases in osmotic pressure and/or specific ion concentrations. During exercise, these changes are added to those induced by muscle contraction. The focal point of all such ionic and osmotic changes is thought to be neural processes within the hypothalamus.", "contents": "Temperature regulation and hypohydration: a singular view. Body temperatures of exercising humans who have been denied water are elevated when compared to hydrated controls. The simplest \"explanation\" for the elevated temperature is a decrease in sensitivity of the sweating mechanism. This and similar \"explanations\" do not direct attention to basic causes but only the result(s) of more fundamental aspects of regulatory physiology. Among the items considered in this speculative presentation are influences of changes in osmolarity, specific ions, peptide hormones, fluid shifts, and muscular contractions during exercise. A hypothesis is offered for consideration in explaining elevations of body temperature in exercise with and without water replacement. In general, the hypothesis relates changes in hypothalamic osmotic pressure and/or ionic constituents with fluid and ionic events in muscle during exercise. The fluid and ionic shifts are probably proportional to the amount of lean body mass engaged in dynamic exercise. Since blood volume has also been shown to be related to lean body mass, similar relative work loads should lead to similar changes in the osmotic and/or ionic environment of the hypothalamus, thus resulting in similar increases in body temperature during exercise. Hypohydration is superimposed on this basic response. Increases in body temperature of resting hypohydrated subjects appear to be due to increases in osmotic pressure and/or specific ion concentrations. During exercise, these changes are added to those induced by muscle contraction. The focal point of all such ionic and osmotic changes is thought to be neural processes within the hypothalamus."}
{"id": "PMID:468649", "title": "Effects of graded reduction of brain blood flow on ventilation in unanesthetized goats.", "content": "The ventilatory effects of graded reductions in brain bloow flow (BBF) were studied in unanesthetized goats. At a BBF of 85% of control (PVO2 = 29.2 Torr, PVCO2 = 47.3 Torr) there were no clear ventilatory effects. At BBF of 70% of control (PVO2 = 25.2, PVCO2 = 50.5) and 50% of control (PVCO2 = 22.3, PVCO2 = 53.0) there was hyperpnea, due primarily to an increase of tidal volume. Further reduction of BBF (avg of 42% of control) first produced intense tachypnea and then (30--40% of control) caused apnea that was reversible. At 50% BBF there was a reduction of brain O2 consumption, (4.67--4.00 ml/min) and an increase in systemic O2 consumption. beta-Adrenergic blockade prevented the increase in systemic O2 consumption and reduced the hyperpnea by two-thirds at 50% BBF; the residual hyperpnea was associated with hypocapnia in contrast to the hyperpnea prior to beta-adrenergic blockade, which was virtually isocapnic. The data suggest that hyperpnea due to brain ischemia is a result of both brain acidosis and systemic hypermetabolism. The similarity of the pattern of responses to that previously reported for progressive carboxyhemoglobinemia suggests that brain hypoxia is a determinant of the ventilatory responses to brain ischemia.", "contents": "Effects of graded reduction of brain blood flow on ventilation in unanesthetized goats. The ventilatory effects of graded reductions in brain bloow flow (BBF) were studied in unanesthetized goats. At a BBF of 85% of control (PVO2 = 29.2 Torr, PVCO2 = 47.3 Torr) there were no clear ventilatory effects. At BBF of 70% of control (PVO2 = 25.2, PVCO2 = 50.5) and 50% of control (PVCO2 = 22.3, PVCO2 = 53.0) there was hyperpnea, due primarily to an increase of tidal volume. Further reduction of BBF (avg of 42% of control) first produced intense tachypnea and then (30--40% of control) caused apnea that was reversible. At 50% BBF there was a reduction of brain O2 consumption, (4.67--4.00 ml/min) and an increase in systemic O2 consumption. beta-Adrenergic blockade prevented the increase in systemic O2 consumption and reduced the hyperpnea by two-thirds at 50% BBF; the residual hyperpnea was associated with hypocapnia in contrast to the hyperpnea prior to beta-adrenergic blockade, which was virtually isocapnic. The data suggest that hyperpnea due to brain ischemia is a result of both brain acidosis and systemic hypermetabolism. The similarity of the pattern of responses to that previously reported for progressive carboxyhemoglobinemia suggests that brain hypoxia is a determinant of the ventilatory responses to brain ischemia."}
{"id": "PMID:468650", "title": "Effects of morphine on ventilatory response to exercise.", "content": "The effects of analgesic doses of morphine on ventilation, arterial blood gas tensions, chemical control of breathing, and the ventilatory response to exercise were studied in six normal subjects. After administration of 0.2 mg/kg morphine, resting ventilation decreased primarily because of a reduction of tidal volume. Ventilatory responses to carbon dioxide and hypoxia were significantly reduced to one-half and one-third of control, respectively. Ventilatory responses at any given level of exercise were significantly reduced after morphine. However, since oxygen consumption during exercise was similarly reduced after morphine, the relationship between ventilation and metabolic rate during steady-state exercise was not altered by the drug. In addition, morphine prolonged the attainment of steady-state ventilation in four of the six subjects, similar to that reported for chemodenervated subjects. The findings suggest that blunting of chemoreception for hypoxia and hypercapnia has no effect upon the link between metabolic rate and ventilation during steady-state exercise, but the hypoxia chemoreflex may be involved in determining the dynamic characteristics of the response.", "contents": "Effects of morphine on ventilatory response to exercise. The effects of analgesic doses of morphine on ventilation, arterial blood gas tensions, chemical control of breathing, and the ventilatory response to exercise were studied in six normal subjects. After administration of 0.2 mg/kg morphine, resting ventilation decreased primarily because of a reduction of tidal volume. Ventilatory responses to carbon dioxide and hypoxia were significantly reduced to one-half and one-third of control, respectively. Ventilatory responses at any given level of exercise were significantly reduced after morphine. However, since oxygen consumption during exercise was similarly reduced after morphine, the relationship between ventilation and metabolic rate during steady-state exercise was not altered by the drug. In addition, morphine prolonged the attainment of steady-state ventilation in four of the six subjects, similar to that reported for chemodenervated subjects. The findings suggest that blunting of chemoreception for hypoxia and hypercapnia has no effect upon the link between metabolic rate and ventilation during steady-state exercise, but the hypoxia chemoreflex may be involved in determining the dynamic characteristics of the response."}
{"id": "PMID:468651", "title": "Ventilatory response to muscular exercise: observations regarding a humoral pathway.", "content": "To assess the role of humoral mechanisms in eliciting the hyperpnea of muscular exercise, anesthetized dogs underwent complete spinal transection at the second lumbar level (L2). Muscular exercise of the denervated hindlimbs was then induced by electrical stimulation. Coincident with hindlimb muscle contraction, oxygen consumption (VO2) increased 173% and ventilation (VE) increased 163%; no statistically significant changes occurred in arterial carbon dioxide tension (PCO2), arterial pH, or arterial oxygen saturation. Similar results were obtained after peripheral chemodenervation and vagotomy plus spinal transection. In order to evaluate the possibility that extracranial receptors mediate the increases in VE elicited by exercise-induced humoral factors, heads of vagotomized L2 spinal-transected dogs were perfused entirely by a support dog with blood of unchanging gas composition via both carotid and both vertebral arteries. The carotid bodies lay within the region of the perfused head; the aortic bodies were denervated. These L2 spinal-transected head-perfused animals still responded to hindlimb exercise with a 156% increase in VO2 and a 122% increase in VE. We conclude that muscular exercise can stimulate VE via humoral factors other than usual chemical stimuli in arterial blood (i.e., PCO2, pH, or O2 saturation). Extracranial receptors (other than conventional peripheral arterial chemoreceptors) appear to mediate a major portion of the increase in VE elicited by exercise-induced humoral factors.", "contents": "Ventilatory response to muscular exercise: observations regarding a humoral pathway. To assess the role of humoral mechanisms in eliciting the hyperpnea of muscular exercise, anesthetized dogs underwent complete spinal transection at the second lumbar level (L2). Muscular exercise of the denervated hindlimbs was then induced by electrical stimulation. Coincident with hindlimb muscle contraction, oxygen consumption (VO2) increased 173% and ventilation (VE) increased 163%; no statistically significant changes occurred in arterial carbon dioxide tension (PCO2), arterial pH, or arterial oxygen saturation. Similar results were obtained after peripheral chemodenervation and vagotomy plus spinal transection. In order to evaluate the possibility that extracranial receptors mediate the increases in VE elicited by exercise-induced humoral factors, heads of vagotomized L2 spinal-transected dogs were perfused entirely by a support dog with blood of unchanging gas composition via both carotid and both vertebral arteries. The carotid bodies lay within the region of the perfused head; the aortic bodies were denervated. These L2 spinal-transected head-perfused animals still responded to hindlimb exercise with a 156% increase in VO2 and a 122% increase in VE. We conclude that muscular exercise can stimulate VE via humoral factors other than usual chemical stimuli in arterial blood (i.e., PCO2, pH, or O2 saturation). Extracranial receptors (other than conventional peripheral arterial chemoreceptors) appear to mediate a major portion of the increase in VE elicited by exercise-induced humoral factors."}
{"id": "PMID:468652", "title": "Vagal effects on histamine, carbachol, and prostaglandin F2 alpha responsiveness in the dog.", "content": "To investigate whether an individual dog's responsiveness to histamine correlates with its responsiveness to other bronchoconstrictor agents and to investigate whether varying vagal effects account for the previously described range of histamine responsiveness, we compared dose-effect relationships of histamine to those of two pharmacological dissimilar agents, carbachol and prostaglandin F2 alpha before and after vagal blockade. There was a highly significant correlation between histamine and both carbachol (P less than 0.001) and prostaglandin F2 alpha (P less than 0.001) responsiveness. The range of responsiveness to prostaglandin F2 alpha was greater than that for histamine or carbachol. When histamine and carbachol were given simultaneously, a purely additive effect was found. Vagal blockade had no significant effect on histamine or carbachol responsiveness, but significantly diminished the responsiveness to prostaglandin F2 alpha; however, it neither narrowed the range nor changed the rank order of responsiveness. We conclude that the range of responsiveness is not specific for any one agent and that vagal mechanisms do not play a role in producing this range.", "contents": "Vagal effects on histamine, carbachol, and prostaglandin F2 alpha responsiveness in the dog. To investigate whether an individual dog's responsiveness to histamine correlates with its responsiveness to other bronchoconstrictor agents and to investigate whether varying vagal effects account for the previously described range of histamine responsiveness, we compared dose-effect relationships of histamine to those of two pharmacological dissimilar agents, carbachol and prostaglandin F2 alpha before and after vagal blockade. There was a highly significant correlation between histamine and both carbachol (P less than 0.001) and prostaglandin F2 alpha (P less than 0.001) responsiveness. The range of responsiveness to prostaglandin F2 alpha was greater than that for histamine or carbachol. When histamine and carbachol were given simultaneously, a purely additive effect was found. Vagal blockade had no significant effect on histamine or carbachol responsiveness, but significantly diminished the responsiveness to prostaglandin F2 alpha; however, it neither narrowed the range nor changed the rank order of responsiveness. We conclude that the range of responsiveness is not specific for any one agent and that vagal mechanisms do not play a role in producing this range."}
{"id": "PMID:468653", "title": "Hemodynamic responses of dog lung lobe to lobar venous occlusion.", "content": "We perfused the left lower lobe of the dog lung with constant flow. When the lobar venous outflow was occluded, the lobar venous pressure rose suddenly to a level somewhere below the arterial pressure, and then the arterial and venous pressures began to rise more slowly. A possible explanation for this response is that, when the outflow was occluded, flow through some downstream segment of the bed ceased. Because flow into the lung continued, the arteriovenous pressure difference after occlusion represents the pressure drop across some upstream segment through which the flow continued. We designated the arteriovenous pressure difference just after outflow occlusion as the upstream pressure drop. The arteriovenous pressure difference before occlusion minus the upstream pressure drop was designated the downstream pressure drop. In an attempt to better understand the meaning of the upstream and downstream pressure drops, we examined the influence of pulmonary vasoconstriction and flow direction on the size of the upstream and downstream pressure drops. We also compared these pressure drops with the pressure drops occurring upstream and downstream from the midpoint of the lobar vascular volume, using the low-viscosity bolus technique. The results indicate that changes in the upstream and downstream pressure drops, as evaluated by outflow occlusion, reflect changes in the lobar arterial and venous resistances.", "contents": "Hemodynamic responses of dog lung lobe to lobar venous occlusion. We perfused the left lower lobe of the dog lung with constant flow. When the lobar venous outflow was occluded, the lobar venous pressure rose suddenly to a level somewhere below the arterial pressure, and then the arterial and venous pressures began to rise more slowly. A possible explanation for this response is that, when the outflow was occluded, flow through some downstream segment of the bed ceased. Because flow into the lung continued, the arteriovenous pressure difference after occlusion represents the pressure drop across some upstream segment through which the flow continued. We designated the arteriovenous pressure difference just after outflow occlusion as the upstream pressure drop. The arteriovenous pressure difference before occlusion minus the upstream pressure drop was designated the downstream pressure drop. In an attempt to better understand the meaning of the upstream and downstream pressure drops, we examined the influence of pulmonary vasoconstriction and flow direction on the size of the upstream and downstream pressure drops. We also compared these pressure drops with the pressure drops occurring upstream and downstream from the midpoint of the lobar vascular volume, using the low-viscosity bolus technique. The results indicate that changes in the upstream and downstream pressure drops, as evaluated by outflow occlusion, reflect changes in the lobar arterial and venous resistances."}
{"id": "PMID:468654", "title": "Adrenal component to pulmonary hypertension induced by elevated cerebrospinal fluid pressure.", "content": "In this study we investigated the possibility that a circulating vasoactive agent contributes to the pulmonary hypertension elicited by elevated cerebrospinal fluid pressure (PCSF) using a denervated canine left lower lung lobe (LLL) preparation that was pump perfused with mixed venous blood at constant flow and venous pressure. Raising the PCSF to an average 190 Torr resulted in a 34.3% increase in LLL inflow pressure. This response was eliminated by adrenal venous occlusion and also by alpha-blockade of the LLL. The results indicated that adrenal catecholamines were responsible for the LLL response. The passively induced elevation of left atrial pressure (Pla) that occurs in the intact animal during elevated PCSF was stimulated in the LLL by raising the outflow pressure. This maneuver attenuated the increase in LLL vascular resistance and suggested that the elevation in Pla seen in the intact animal could mask humorally mediated responses of the magnitude we observed.", "contents": "Adrenal component to pulmonary hypertension induced by elevated cerebrospinal fluid pressure. In this study we investigated the possibility that a circulating vasoactive agent contributes to the pulmonary hypertension elicited by elevated cerebrospinal fluid pressure (PCSF) using a denervated canine left lower lung lobe (LLL) preparation that was pump perfused with mixed venous blood at constant flow and venous pressure. Raising the PCSF to an average 190 Torr resulted in a 34.3% increase in LLL inflow pressure. This response was eliminated by adrenal venous occlusion and also by alpha-blockade of the LLL. The results indicated that adrenal catecholamines were responsible for the LLL response. The passively induced elevation of left atrial pressure (Pla) that occurs in the intact animal during elevated PCSF was stimulated in the LLL by raising the outflow pressure. This maneuver attenuated the increase in LLL vascular resistance and suggested that the elevation in Pla seen in the intact animal could mask humorally mediated responses of the magnitude we observed."}
{"id": "PMID:468655", "title": "Human pulmonary resistance: effect of frequency and gas physical properties.", "content": "We have investigated a new technique that could be specific for detecting small airway diseases. We measured resistance in nine subjects, evaluating the effect of breathing 80% He-20%O2, air, and 80% SF6-20% O2 at different flow rates (0.25--1.01 l/s) and frequencies (4--12 Hz). To test the sensitivity and specificity of this new technique, we used intravenous histamine infusion to cause peripheral constriction. During histamine infusion six of nine subjects showed the following changes: 1) dynamic compliance decreased; 2) subjects developed frequency dependence of compliance, and 3) there was no significant change in pulmonary resistance or Rrs (respiratory resistance) at 4 Hz. These data suggested a time-constant discrepancy in the periphery due to histamine infusion. These six subjects also developed frequency dependence of resistance (delta Rrs from 4 to 12 Hz), which was significant when breathing air (-22 +/- 6%) and maximal when we used He-O2 (-32 +/- 3%). We conclude that He-O2 can improve the specificity of frequency dependence of Rrs for detecting events occurring in the peripheral airways.", "contents": "Human pulmonary resistance: effect of frequency and gas physical properties. We have investigated a new technique that could be specific for detecting small airway diseases. We measured resistance in nine subjects, evaluating the effect of breathing 80% He-20%O2, air, and 80% SF6-20% O2 at different flow rates (0.25--1.01 l/s) and frequencies (4--12 Hz). To test the sensitivity and specificity of this new technique, we used intravenous histamine infusion to cause peripheral constriction. During histamine infusion six of nine subjects showed the following changes: 1) dynamic compliance decreased; 2) subjects developed frequency dependence of compliance, and 3) there was no significant change in pulmonary resistance or Rrs (respiratory resistance) at 4 Hz. These data suggested a time-constant discrepancy in the periphery due to histamine infusion. These six subjects also developed frequency dependence of resistance (delta Rrs from 4 to 12 Hz), which was significant when breathing air (-22 +/- 6%) and maximal when we used He-O2 (-32 +/- 3%). We conclude that He-O2 can improve the specificity of frequency dependence of Rrs for detecting events occurring in the peripheral airways."}
{"id": "PMID:468656", "title": "Respiratory impedance and derived parameters in young children by forced random noise.", "content": "The frequency dependence of total respiratory impedance during spontaneous breathing was measured repeatedly in 16 children (3--5 yr old) over a 3-mo period using forced random noise and spectral analysis. Total respiratory resistance, compliance, and inertance, which were calculated fromthe impedance data using regression analysis with a second-order model, had overall mean values +/- SD of 5.61 +/- 0.49 cmH2O.l-1.s, 403 +/- 1.04 ml.cmH2O-1, and 0.0120 +/- 0.0024 cmH2O.l-1.s2, respectively. Linear regression analysis showed that resistance and compliance correlated with either height or forced vital capacity with r values in the range 0.77--0.89. Analysis of measurement variability suggested that resistance measurements had, on the average, a coefficient of variation of 15%; corresponding values for compliance and inertance measurements were 25%.", "contents": "Respiratory impedance and derived parameters in young children by forced random noise. The frequency dependence of total respiratory impedance during spontaneous breathing was measured repeatedly in 16 children (3--5 yr old) over a 3-mo period using forced random noise and spectral analysis. Total respiratory resistance, compliance, and inertance, which were calculated fromthe impedance data using regression analysis with a second-order model, had overall mean values +/- SD of 5.61 +/- 0.49 cmH2O.l-1.s, 403 +/- 1.04 ml.cmH2O-1, and 0.0120 +/- 0.0024 cmH2O.l-1.s2, respectively. Linear regression analysis showed that resistance and compliance correlated with either height or forced vital capacity with r values in the range 0.77--0.89. Analysis of measurement variability suggested that resistance measurements had, on the average, a coefficient of variation of 15%; corresponding values for compliance and inertance measurements were 25%."}
{"id": "PMID:468657", "title": "Improved measurements of shear modulus and pleural membrane tension of the lung.", "content": "The continuum solution for the deformation of an elastic half space covered by a membrane is used to interpret measurements of the indentation of lung lobes under a column of fluid. The shear modulus mu of the underlying parenchyma is found to be approximately 0.7 times transpulmonary pressure, independent of species size. The tension in the pleural membrane T increases rapidly with increasing membrane area. For dog lungs, the value of T is 10(3) to 10(4) dyn/cm. For the larger species tested, pigs and horses, T is larger. The continuum solution shows that a concentrated force applied to the pleural surface is distributed over a distance T/mu as it is transmitted across the pleural membrane. The membrane is important in determining the displacement produced by forces that act within a region that is small compared to this distance, approximately 2 cm for dog lungs. By comparing the tension-area curve of the pleural membrane with the pressure-volume curve of the lobe, it is found that the pleural membrane contributes about 20% of the work done by the lung during deflation.", "contents": "Improved measurements of shear modulus and pleural membrane tension of the lung. The continuum solution for the deformation of an elastic half space covered by a membrane is used to interpret measurements of the indentation of lung lobes under a column of fluid. The shear modulus mu of the underlying parenchyma is found to be approximately 0.7 times transpulmonary pressure, independent of species size. The tension in the pleural membrane T increases rapidly with increasing membrane area. For dog lungs, the value of T is 10(3) to 10(4) dyn/cm. For the larger species tested, pigs and horses, T is larger. The continuum solution shows that a concentrated force applied to the pleural surface is distributed over a distance T/mu as it is transmitted across the pleural membrane. The membrane is important in determining the displacement produced by forces that act within a region that is small compared to this distance, approximately 2 cm for dog lungs. By comparing the tension-area curve of the pleural membrane with the pressure-volume curve of the lobe, it is found that the pleural membrane contributes about 20% of the work done by the lung during deflation."}
{"id": "PMID:468658", "title": "Distribution of regional volumes and ventilation in excised canine lobes.", "content": "A linear elasticity solution for the gravitational deformation of excised lungs was obtained. The accuracy of our solution was examined by comparing predicted and measured displacements of markers glued to the surface of canine lower lobes. The equations describing the strains in a lobe were used to predict the distribution of regional volumes and the slope of phase III (S3) of a single-breath oxygen (SBO2) test. The analysis predicted a negative S3. However, S3 was found to be positive in the five lobes tested, suggesting that factors other than gravity were responsible for the observed pattern of ventilation. In SBO2 tests repeated with increasing delays at end inflation, S3 progressively decreased, became negative, and was eventually abolished. Our equations predicted the most negative observed S3 well. We conclude that continuum mechanics can be used to describe the gravitational deformation of lungs and the resulting effect on ventilation distribution.", "contents": "Distribution of regional volumes and ventilation in excised canine lobes. A linear elasticity solution for the gravitational deformation of excised lungs was obtained. The accuracy of our solution was examined by comparing predicted and measured displacements of markers glued to the surface of canine lower lobes. The equations describing the strains in a lobe were used to predict the distribution of regional volumes and the slope of phase III (S3) of a single-breath oxygen (SBO2) test. The analysis predicted a negative S3. However, S3 was found to be positive in the five lobes tested, suggesting that factors other than gravity were responsible for the observed pattern of ventilation. In SBO2 tests repeated with increasing delays at end inflation, S3 progressively decreased, became negative, and was eventually abolished. Our equations predicted the most negative observed S3 well. We conclude that continuum mechanics can be used to describe the gravitational deformation of lungs and the resulting effect on ventilation distribution."}
{"id": "PMID:468659", "title": "Influence of exercise and CO2 on breathing pattern of normal man.", "content": "Ventilatory patterns during rest, CO2 inhalation (2, 3, and 4%) and three levels of exercise were analyzed in supine men using a canopy system for noninvasive measurements. Changes in tidal volume (VT) and breathing frequency (f) with equal increases in minute ventilation (VE) differed significantly during exercise and CO2 inhalation. Increases in VE during exercise was accompanied by increases in VT and f. During CO2 inhalation, the change in frequency was less than during exercise. However, when analyzed in terms of inspiratory flow (VT/TI) and inspiratory duty cycle (TI/Ttot), the response to both stimuli was similar. With increases to twice control VE both TI/Ttot and VT/VI increased. Thereafter only VTTI increased with increasing VE. At rest, inspiratory time on a breath by breath basis increased minimally with VT, while changes in inspiratory flow accounted for the variability in VT. These two respiratory stimulants appear to increase ventilation through different mechanisms when analyzed in terms of VT and f. However, changes in inspiratory flow and duty cycle are similar in both.", "contents": "Influence of exercise and CO2 on breathing pattern of normal man. Ventilatory patterns during rest, CO2 inhalation (2, 3, and 4%) and three levels of exercise were analyzed in supine men using a canopy system for noninvasive measurements. Changes in tidal volume (VT) and breathing frequency (f) with equal increases in minute ventilation (VE) differed significantly during exercise and CO2 inhalation. Increases in VE during exercise was accompanied by increases in VT and f. During CO2 inhalation, the change in frequency was less than during exercise. However, when analyzed in terms of inspiratory flow (VT/TI) and inspiratory duty cycle (TI/Ttot), the response to both stimuli was similar. With increases to twice control VE both TI/Ttot and VT/VI increased. Thereafter only VTTI increased with increasing VE. At rest, inspiratory time on a breath by breath basis increased minimally with VT, while changes in inspiratory flow accounted for the variability in VT. These two respiratory stimulants appear to increase ventilation through different mechanisms when analyzed in terms of VT and f. However, changes in inspiratory flow and duty cycle are similar in both."}
{"id": "PMID:468660", "title": "Heat and acute dehydration effects on acceleration response in man.", "content": "Though heat and dehydration each impair acceleration tolerance, interactions among these stresses have not previously been studied. Seven men were dehydrated in heat by 0, 1, and 3% of body weight before a series of +Gz, gradual-onset centrifuge runs with the capsule first 38 degrees C, then 20 degrees C. Heat alone raised heart rate by 6.5 beats/min independent of other stresses. Dehydration and acceleration appeared to act synergistically in raising HR. Heat lowered relaxed G tolerance by 0.3 G; dehydration tended to lower G tolerance and increased the variability of response to heat. A high-tolerance subgroup (n = 4) could normally sustain +7 Gz for 60 s with anti-G suit and straining, but 3% dehydration reduced mean time to 35 s. Dehydration was associated with a decrease in the loss of plasma volume at 7 G. Heat-induced tolerance loss appears similar for both gradual- and rapid-onset centrifuge profiles. In contrast, dehydration effects are greater in rapid-onset runs, evidence that normal anti-G protective mechanisms can partly counteract the effect of fluid deficit. The results are relevant for crew members of high-performance aircraft, where unexpected diminution of their normally high G tolerance can have disastrous consequences.", "contents": "Heat and acute dehydration effects on acceleration response in man. Though heat and dehydration each impair acceleration tolerance, interactions among these stresses have not previously been studied. Seven men were dehydrated in heat by 0, 1, and 3% of body weight before a series of +Gz, gradual-onset centrifuge runs with the capsule first 38 degrees C, then 20 degrees C. Heat alone raised heart rate by 6.5 beats/min independent of other stresses. Dehydration and acceleration appeared to act synergistically in raising HR. Heat lowered relaxed G tolerance by 0.3 G; dehydration tended to lower G tolerance and increased the variability of response to heat. A high-tolerance subgroup (n = 4) could normally sustain +7 Gz for 60 s with anti-G suit and straining, but 3% dehydration reduced mean time to 35 s. Dehydration was associated with a decrease in the loss of plasma volume at 7 G. Heat-induced tolerance loss appears similar for both gradual- and rapid-onset centrifuge profiles. In contrast, dehydration effects are greater in rapid-onset runs, evidence that normal anti-G protective mechanisms can partly counteract the effect of fluid deficit. The results are relevant for crew members of high-performance aircraft, where unexpected diminution of their normally high G tolerance can have disastrous consequences."}
{"id": "PMID:468661", "title": "Equation of motion of a cyclist.", "content": "Tractional resistance (RT, N) was determined by towing two cyclists on a racing bike in \"fully dropped\" posture in calm air on a flat track at constant speed (5--16.5 m/s). RT increased with the air velocity (v, m/s): RT = 3.2 + 0.19 V2. The constant 3.2 N is interpreted as the rolling resistance and the term increasing with v2 as the air resistance. For a given posture this is a function of the body surface (SA, m2), the air temperature (T, degree K), and barometric pressure (PB, Torr). The mechanical power output (W, W) can then be described as a function of the air (v) and ground (s) speed: W = 4.5.10(-2) Ps + 4.1.10(-2) SA (PB/T)v2 s, where P is the overall weight in kg. With a mechanical efficiency of 0.25, the energy expenditure rate (VO2, ml/s) is given by: VO2 = 8.6.10(-3) Ps + 7.8.10(-3) SA (PB/T)v2 s (1 ml O2 = 20.9 J). As the decrease of VO2max with altitude is known from the literature, this last equation allows the calculation of the optimal altitude for top aerobic performance. The prediction derived from this equation is consistent with the present 1-h world record.", "contents": "Equation of motion of a cyclist. Tractional resistance (RT, N) was determined by towing two cyclists on a racing bike in \"fully dropped\" posture in calm air on a flat track at constant speed (5--16.5 m/s). RT increased with the air velocity (v, m/s): RT = 3.2 + 0.19 V2. The constant 3.2 N is interpreted as the rolling resistance and the term increasing with v2 as the air resistance. For a given posture this is a function of the body surface (SA, m2), the air temperature (T, degree K), and barometric pressure (PB, Torr). The mechanical power output (W, W) can then be described as a function of the air (v) and ground (s) speed: W = 4.5.10(-2) Ps + 4.1.10(-2) SA (PB/T)v2 s, where P is the overall weight in kg. With a mechanical efficiency of 0.25, the energy expenditure rate (VO2, ml/s) is given by: VO2 = 8.6.10(-3) Ps + 7.8.10(-3) SA (PB/T)v2 s (1 ml O2 = 20.9 J). As the decrease of VO2max with altitude is known from the literature, this last equation allows the calculation of the optimal altitude for top aerobic performance. The prediction derived from this equation is consistent with the present 1-h world record."}
{"id": "PMID:468662", "title": "Effects of endurance training on left ventricular dimensions in healthy men.", "content": "Echocardiography was employed to measure the serial effects of jogging on resting left ventricular dimensions and function. Twelve men were exercised (mean age 36.8 yr) and 10 served as controls (mean age 34.8 yr). Increases of 14 and 18% were observed for predicted aerobic capacity in the training group (TG) after 3 and 6 mo of training, respectively; the control group (CG) displayed a small detraining effect. Echocardiographic findings included a significant (P less than 0.05) reduction in resting heart rate and a moderate increase in stroke volume (SV) in the TG compared to the CG. The increased SV appeared to be due to increased end-diastolic dimensions (LVIDd, LVEDV), secondary to greater ventricular filling rather than a more vigorous ventricular contraction. Posterior wall thickness, septal thickness, and calculated left ventricular muscle mass were not significantly increased in the TG compared to CG after either 3 or 6 mo training. It was concluded that left ventricular structure and resting contractile status are not altered by 6 mo of jogging training in healthy, previously sedentary men.", "contents": "Effects of endurance training on left ventricular dimensions in healthy men. Echocardiography was employed to measure the serial effects of jogging on resting left ventricular dimensions and function. Twelve men were exercised (mean age 36.8 yr) and 10 served as controls (mean age 34.8 yr). Increases of 14 and 18% were observed for predicted aerobic capacity in the training group (TG) after 3 and 6 mo of training, respectively; the control group (CG) displayed a small detraining effect. Echocardiographic findings included a significant (P less than 0.05) reduction in resting heart rate and a moderate increase in stroke volume (SV) in the TG compared to the CG. The increased SV appeared to be due to increased end-diastolic dimensions (LVIDd, LVEDV), secondary to greater ventricular filling rather than a more vigorous ventricular contraction. Posterior wall thickness, septal thickness, and calculated left ventricular muscle mass were not significantly increased in the TG compared to CG after either 3 or 6 mo training. It was concluded that left ventricular structure and resting contractile status are not altered by 6 mo of jogging training in healthy, previously sedentary men."}
{"id": "PMID:468663", "title": "Effect of heparin or fibrinogen depletion on lung fluid balance in sheep after emboli.", "content": "Investigators have proposed that fibrinogen, fibrin, or their degradation products are essential for the increased lung vascular permeability to fluid and protein that may occur after microemboli. To test this hypothesis, we used 20 anesthetized ventilated sheep in which we measured lung lymph flow, pulmonary artery and left atrial pressures, thermodilution cardiac output, and lymph/plasma protein concentrations. We injected glass bead microemboli (200 micrometers diam) sufficient to raise pulmonary vascular resistance to three times base-line values and cause increased lung lymph flow with a parallel increase in protein clearance, which is characteristic of increased lung vascular permeability. Neither large doses of heparin (3,000 U/kg) nor fibrinogen depletion with viper venom (ancrod, 2 U/kg), by themselves, affected steady-state pulmonary hemodynamics or lung fluid balance. These treatments prior to giving sufficient amounts of emboli to triple the pulmonary vascular resistance did not prevent the increased lung vascular permeability. We conclude that neither fibrin deposition nor degradation are essential to microembolic lung vascular injury in sheep.", "contents": "Effect of heparin or fibrinogen depletion on lung fluid balance in sheep after emboli. Investigators have proposed that fibrinogen, fibrin, or their degradation products are essential for the increased lung vascular permeability to fluid and protein that may occur after microemboli. To test this hypothesis, we used 20 anesthetized ventilated sheep in which we measured lung lymph flow, pulmonary artery and left atrial pressures, thermodilution cardiac output, and lymph/plasma protein concentrations. We injected glass bead microemboli (200 micrometers diam) sufficient to raise pulmonary vascular resistance to three times base-line values and cause increased lung lymph flow with a parallel increase in protein clearance, which is characteristic of increased lung vascular permeability. Neither large doses of heparin (3,000 U/kg) nor fibrinogen depletion with viper venom (ancrod, 2 U/kg), by themselves, affected steady-state pulmonary hemodynamics or lung fluid balance. These treatments prior to giving sufficient amounts of emboli to triple the pulmonary vascular resistance did not prevent the increased lung vascular permeability. We conclude that neither fibrin deposition nor degradation are essential to microembolic lung vascular injury in sheep."}
{"id": "PMID:468664", "title": "Isobaric gas counterdiffusion in rabbit eye.", "content": "The superficial isobaric gas counterdiffusion phenomenon, which produces dermal lesions and lethal gas embolism, was investigated at sea level and 2 ATA for effects on the eye of the New Zealand White rabbit. The animals breathed an N2O-O2 mixture by mask and were surrounded by a He environment. There was no gas lesion formation in the conjunctiva or cornea and no gas bubble formation in the anterior compartment of the eye in any of the experimental animals, even at the maximal 8-h exposure. In contrast to the eye, the skin of these animals showed progressive gas-filled lesions after 3 h at 1 ATA. Reasons for the relative insensitivity of the structures of the eye to the counter-diffusion process are discussed.", "contents": "Isobaric gas counterdiffusion in rabbit eye. The superficial isobaric gas counterdiffusion phenomenon, which produces dermal lesions and lethal gas embolism, was investigated at sea level and 2 ATA for effects on the eye of the New Zealand White rabbit. The animals breathed an N2O-O2 mixture by mask and were surrounded by a He environment. There was no gas lesion formation in the conjunctiva or cornea and no gas bubble formation in the anterior compartment of the eye in any of the experimental animals, even at the maximal 8-h exposure. In contrast to the eye, the skin of these animals showed progressive gas-filled lesions after 3 h at 1 ATA. Reasons for the relative insensitivity of the structures of the eye to the counter-diffusion process are discussed."}
{"id": "PMID:468665", "title": "Subcutaneous tissue gas space pressure during superficial isobaric counterdiffusion.", "content": "Changes in subcutaneous tissue pressure caused by N2O-He, 1-ATA isobaric counterdiffusion gas phase development were measured. Only the ears of New Zealand White rabbits were subjected to counterdiffusion. The rabbits breathed a mixture of 80% N2O-20% O2 while their ears alone were surrounded by He and the rest of their bodies continued to be surrounded by air. Subcutaneous pressure changes were transmitted to the transducer-recorded system via a fluid-filled subcutaneous needle. When the gas phase developed in subcutaneous tissue, pressure rose and a maximum pressure (Pmax) was reached. Pmax in the counterdiffused ear was 48 +/- 10 (SD) Torr, and mean time to reach Pmax was 75 +/- 10 (SD) min. The findings are discussed in relation to the pathological processes of isobaric inert gas counterdiffusion.", "contents": "Subcutaneous tissue gas space pressure during superficial isobaric counterdiffusion. Changes in subcutaneous tissue pressure caused by N2O-He, 1-ATA isobaric counterdiffusion gas phase development were measured. Only the ears of New Zealand White rabbits were subjected to counterdiffusion. The rabbits breathed a mixture of 80% N2O-20% O2 while their ears alone were surrounded by He and the rest of their bodies continued to be surrounded by air. Subcutaneous pressure changes were transmitted to the transducer-recorded system via a fluid-filled subcutaneous needle. When the gas phase developed in subcutaneous tissue, pressure rose and a maximum pressure (Pmax) was reached. Pmax in the counterdiffused ear was 48 +/- 10 (SD) Torr, and mean time to reach Pmax was 75 +/- 10 (SD) min. The findings are discussed in relation to the pathological processes of isobaric inert gas counterdiffusion."}
{"id": "PMID:468666", "title": "Effect of inhalate thermal conductivity and high O2 in producing hypothermia.", "content": "The effect of an increase in inhalate thermal conductivity and the fraction of inspiratory O2 (FIO2) on the rate of cooling and rewarming using a surface-inhalate heat exchange method was evaluated. Male New Zealand White rabbits were divided into three groups: those ventilated with air, those with 20% O2 + 80% He, and those with 100% O2. All animals were cooled to an esophageal temperature of 22.5 degrees C (or for 180 min maximum). Following a 15-min exposure to room air, the animals were connected to the humidifying and warming system. He-O2 had the highest thermal conductivity and the animals ventilated with it had the fastest cooling rate. One hundred percent O2 and room air had similar thermal conductivities, but the animals ventilated with 100% O2 had significantly lower cooling rates. These data indicate that, while maintaining a constant surface heart exchange, the rate of heat exchange across the lung can be modified by altering the thermal conductivity of the inhalate gas mixture. Total heat exchange can also be modified by hyperoxemia-induced hemodynamic changes.", "contents": "Effect of inhalate thermal conductivity and high O2 in producing hypothermia. The effect of an increase in inhalate thermal conductivity and the fraction of inspiratory O2 (FIO2) on the rate of cooling and rewarming using a surface-inhalate heat exchange method was evaluated. Male New Zealand White rabbits were divided into three groups: those ventilated with air, those with 20% O2 + 80% He, and those with 100% O2. All animals were cooled to an esophageal temperature of 22.5 degrees C (or for 180 min maximum). Following a 15-min exposure to room air, the animals were connected to the humidifying and warming system. He-O2 had the highest thermal conductivity and the animals ventilated with it had the fastest cooling rate. One hundred percent O2 and room air had similar thermal conductivities, but the animals ventilated with 100% O2 had significantly lower cooling rates. These data indicate that, while maintaining a constant surface heart exchange, the rate of heat exchange across the lung can be modified by altering the thermal conductivity of the inhalate gas mixture. Total heat exchange can also be modified by hyperoxemia-induced hemodynamic changes."}
{"id": "PMID:468667", "title": "Initiation of each avian inspiration by a CO2 threshold mechanism.", "content": "The avian respiratory oscillator has been investigated in a unidirectionally ventilated chicken by changing the dynamic pattern of inflow CO2 concentration (FCO2). Stimulation with periodic FCO2 results in a one-to-one synchronization of the respiratory movements that we have called pacing (Respir. Physiol. 22: 167--177, 1974). A two-parameter CO2 threshold model is proposed to explain this behavior. The model states that when FCO2 reaches a threshold level (L), it initiates the beginning of inspiration a constant time interval (LB) later. According to this model, when a triangular FCO2 concentration is used to synchronize the breathing pattern, the time from the minimum of the wave form to the beginning of inspiration (C-B interval) is dependent on the mean value and the rate of rise of FCO2 as determined by period and amplitude of the triangle. Particularly interesting is the prediction that the direction of the relationship (increasing or decreasing) between FCO2 amplitude and the C-B interval is dependent on whether the mean value of FCO2 is above or below the threshold level. Experimental data obtained during amplitude changes support the above prediction.", "contents": "Initiation of each avian inspiration by a CO2 threshold mechanism. The avian respiratory oscillator has been investigated in a unidirectionally ventilated chicken by changing the dynamic pattern of inflow CO2 concentration (FCO2). Stimulation with periodic FCO2 results in a one-to-one synchronization of the respiratory movements that we have called pacing (Respir. Physiol. 22: 167--177, 1974). A two-parameter CO2 threshold model is proposed to explain this behavior. The model states that when FCO2 reaches a threshold level (L), it initiates the beginning of inspiration a constant time interval (LB) later. According to this model, when a triangular FCO2 concentration is used to synchronize the breathing pattern, the time from the minimum of the wave form to the beginning of inspiration (C-B interval) is dependent on the mean value and the rate of rise of FCO2 as determined by period and amplitude of the triangle. Particularly interesting is the prediction that the direction of the relationship (increasing or decreasing) between FCO2 amplitude and the C-B interval is dependent on whether the mean value of FCO2 is above or below the threshold level. Experimental data obtained during amplitude changes support the above prediction."}
{"id": "PMID:468668", "title": "A closed lung system study of inert gas absorption.", "content": "The demonstration that the rate of rise of the alveolar fraction of nitrous oxide is enhanced when the inspired N2O concentration is high is termed the \"concentration effect.\" A similar effect on a second gas has been termed the \"second gas effect\". These effects have been observed in open systems and attributed to differential changes between inspired and expired ventilation. The purpose of this investigation is to study these effects in a closed system. A breath-holding maneuver was utilized with a high and a low N2O concentration in argon and oxygen. The results indicate that breath holding with a high N2O concentration \"concentrates\" both the alveolar fraction of N2O and argon. These results are attributable to alveolar volume shrinkage as a consequence of the large absorption of N2O by the pulmonary blood. A mathematical model verifies this interpretation and suggests that volume shrinkage can be important in breath-holding maneuvers designed for noninvasive measurement of cardiac output and lung tissue volume.", "contents": "A closed lung system study of inert gas absorption. The demonstration that the rate of rise of the alveolar fraction of nitrous oxide is enhanced when the inspired N2O concentration is high is termed the \"concentration effect.\" A similar effect on a second gas has been termed the \"second gas effect\". These effects have been observed in open systems and attributed to differential changes between inspired and expired ventilation. The purpose of this investigation is to study these effects in a closed system. A breath-holding maneuver was utilized with a high and a low N2O concentration in argon and oxygen. The results indicate that breath holding with a high N2O concentration \"concentrates\" both the alveolar fraction of N2O and argon. These results are attributable to alveolar volume shrinkage as a consequence of the large absorption of N2O by the pulmonary blood. A mathematical model verifies this interpretation and suggests that volume shrinkage can be important in breath-holding maneuvers designed for noninvasive measurement of cardiac output and lung tissue volume."}
{"id": "PMID:468669", "title": "Effect of methacholine chloride on rheology and transport of canine tracheal mucus.", "content": "The effect of methacholine chloride (M) on tracheal mucus was investigated in three conscious tracheostomized dogs. Aerosols of M in concentrations of 2--32 mg/ml were delivered intratracheally for 1 min. Mucus was sampled with a cytology brush at 2 min postchallenge and at irregular intervals thereafter. The mechanical properties of each sample were determined in the magnetic microrheometer, and correlated with mucociliary transportability as assayed by the frog palate technique. With high doses of M, there was an increase in volume of secretion collected per unit time. The elastic modulus (G') at 2 min postchallenge went up (to 1.5 x 2.3 x control for 16 and 32 mg/ml, respectively) then fell below control before returning to base line after 30 or 45 min. With low doses of M (2--8 mg/ml) the secretion rate was also above control, but only a decrease in G' (to 0.54 x control) was observed. The decrease in G' at low doses did not significantly alter the frog palate transport rate; however, the increase at higher doses did impede mucociliary transport.", "contents": "Effect of methacholine chloride on rheology and transport of canine tracheal mucus. The effect of methacholine chloride (M) on tracheal mucus was investigated in three conscious tracheostomized dogs. Aerosols of M in concentrations of 2--32 mg/ml were delivered intratracheally for 1 min. Mucus was sampled with a cytology brush at 2 min postchallenge and at irregular intervals thereafter. The mechanical properties of each sample were determined in the magnetic microrheometer, and correlated with mucociliary transportability as assayed by the frog palate technique. With high doses of M, there was an increase in volume of secretion collected per unit time. The elastic modulus (G') at 2 min postchallenge went up (to 1.5 x 2.3 x control for 16 and 32 mg/ml, respectively) then fell below control before returning to base line after 30 or 45 min. With low doses of M (2--8 mg/ml) the secretion rate was also above control, but only a decrease in G' (to 0.54 x control) was observed. The decrease in G' at low doses did not significantly alter the frog palate transport rate; however, the increase at higher doses did impede mucociliary transport."}
{"id": "PMID:468670", "title": "Diffusing capacity at different lung volumes during breath holding and rebreathing.", "content": "Single-breath diffusing capacity of the lung for carbon monoxide (DLCO) increases as lung volume increases above functional residual capacity (FRC). However, the physiological mechanism responsible for this increase remains controversial. This volume dependence of diffusing capacity could reflect changing regional distribution of inspired air as lung volume increases rather than a change in capillary blood volume or surface area for gas exchange. We measured DLCO during breath holding and during rebreathing with a technique employed to mix respired gases throughout the lung thereby minimizing regional distribution differences. Measurements were made 1,500 ml above FRC and near total lung capacity (TLC). Breath holding DLCO was 18% higher near TLC than at 1,500 ml above FRC (P less than 0.05). Rebreathing DLCO was 16% higher near TCL than at 1,500 ml above FRC (P less than 0.01). Equality of results by the two techniques indicates that changes in DLCO with lung volume are not a consequence of the changing distribution of inspired air. Our results are compatible with the hypothesis that effective surface area of the lung increases as lung volume expands.", "contents": "Diffusing capacity at different lung volumes during breath holding and rebreathing. Single-breath diffusing capacity of the lung for carbon monoxide (DLCO) increases as lung volume increases above functional residual capacity (FRC). However, the physiological mechanism responsible for this increase remains controversial. This volume dependence of diffusing capacity could reflect changing regional distribution of inspired air as lung volume increases rather than a change in capillary blood volume or surface area for gas exchange. We measured DLCO during breath holding and during rebreathing with a technique employed to mix respired gases throughout the lung thereby minimizing regional distribution differences. Measurements were made 1,500 ml above FRC and near total lung capacity (TLC). Breath holding DLCO was 18% higher near TLC than at 1,500 ml above FRC (P less than 0.05). Rebreathing DLCO was 16% higher near TCL than at 1,500 ml above FRC (P less than 0.01). Equality of results by the two techniques indicates that changes in DLCO with lung volume are not a consequence of the changing distribution of inspired air. Our results are compatible with the hypothesis that effective surface area of the lung increases as lung volume expands."}
{"id": "PMID:468671", "title": "Thoracoabdominal mechanics during relaxed and forced vital capacity.", "content": "Thoracoabdominal configuration, intrathoracic (esophageal), intra-abdominal (gastric), and transdiaphragmatic pressures were studied in six normal upright subjects during relaxed (RVC) and forced vital capacity (FVC). Chest wall configuration showed substantial departure from its relaxation characteristics during FVC. Paradoxical (outward) movement was recorded for a low lateral diameter of the rib cage (on the costal margin) at high volume during RVC and during most of FVC, while the other rib cage dimensions were decreasing. Transdiaphragmatic pressure was positive during most of the FVC, particularly toward RV, reflecting active contraction of the diaphragm. We conclude that diaphragmatic activity modulates forced expiration and that the chest wall may influence the FVC maneuver.", "contents": "Thoracoabdominal mechanics during relaxed and forced vital capacity. Thoracoabdominal configuration, intrathoracic (esophageal), intra-abdominal (gastric), and transdiaphragmatic pressures were studied in six normal upright subjects during relaxed (RVC) and forced vital capacity (FVC). Chest wall configuration showed substantial departure from its relaxation characteristics during FVC. Paradoxical (outward) movement was recorded for a low lateral diameter of the rib cage (on the costal margin) at high volume during RVC and during most of FVC, while the other rib cage dimensions were decreasing. Transdiaphragmatic pressure was positive during most of the FVC, particularly toward RV, reflecting active contraction of the diaphragm. We conclude that diaphragmatic activity modulates forced expiration and that the chest wall may influence the FVC maneuver."}
{"id": "PMID:468672", "title": "Variability of airway responses to inhaled histamine in normal subjects.", "content": "Dose-response curves to inhaled histamine were studied in 12 normal subjects. Pulmonary resistance (RL) and dynamic compliance (Cdyn) were measured during tidal breathing, and maximum expiratory flow rates, at an absolute lung volume corresponding to 40% of control vital capacity, were obtained during forced expiration from tidal end inspiration (Vmax40p) and from total lung capacity (Vmax40c). Threshold was defined as the histamine dose at which a departure from the range of normal measurements was observed. RL and Vmax40p indicated lowest threshold values, which varied by a factor of 32 and 38, respectively. There was no correlation between reactivity, which reflects the slope of the dose-response curve beyond the threshold dose, and threshold doses, nor between the initial RL (normalized for lung volume) and either threshold or reactivity. In eight subjects, restudied on two occasions after 10 mg propranolol or after saline, injected in a double-blind manner, there was no change in the dose-response curves. These results indicate that different indices of bronchoconstriction may yield different dose-response curves and hence different sensitivities. In addition, a wide variation of airway responses to inhaled histamine exists in the normal population and beta-blockade does not influence this variability.", "contents": "Variability of airway responses to inhaled histamine in normal subjects. Dose-response curves to inhaled histamine were studied in 12 normal subjects. Pulmonary resistance (RL) and dynamic compliance (Cdyn) were measured during tidal breathing, and maximum expiratory flow rates, at an absolute lung volume corresponding to 40% of control vital capacity, were obtained during forced expiration from tidal end inspiration (Vmax40p) and from total lung capacity (Vmax40c). Threshold was defined as the histamine dose at which a departure from the range of normal measurements was observed. RL and Vmax40p indicated lowest threshold values, which varied by a factor of 32 and 38, respectively. There was no correlation between reactivity, which reflects the slope of the dose-response curve beyond the threshold dose, and threshold doses, nor between the initial RL (normalized for lung volume) and either threshold or reactivity. In eight subjects, restudied on two occasions after 10 mg propranolol or after saline, injected in a double-blind manner, there was no change in the dose-response curves. These results indicate that different indices of bronchoconstriction may yield different dose-response curves and hence different sensitivities. In addition, a wide variation of airway responses to inhaled histamine exists in the normal population and beta-blockade does not influence this variability."}
{"id": "PMID:468673", "title": "Tolerance of estrogen-treated rats to acute cold exposure.", "content": "Female rats treated chronically with ethynylestradiol (36 micrograms/kg per day) alone, and in combination with the progestational agent, norethynodrel (253 micrograms/kg per day), cooled significantly faster than controls when lightly restrained and exposed to air at 5 degrees C. Rate of cooling of rats given only norethynodrel was similar to that of the control group. In other studies, rate of oxygen consumption was determined for all groups during acute exposure to cold (14 degrees C). All estrogen-treated groups achieved the same maximal rate of oxygen consumption as control and norethynodrel-treated groups during cold exposure, but cooled significantly faster. Two groups of female rats were treated chronically with ethynylestradiol at two separate doses (36 and 61 micrograms/kg per day). An untreated group served as controls. Rate of oxygen consumption of all animals were measured during restraint and exposure to cold (18 degrees C). The estrogen-treated groups again achieved the same maximal rate of oxygen consumption as the control group, but also cooled significantly faster despite the fact that the cold stress was less severe than in the previous experiment. That estrogen-treated rats cooled faster than controls in both studies despite achieving a maximal rate of heat production which did not differ from controls suggests that reduced cold tolerance of estrogen-treated rats may be related to increased heat loss.", "contents": "Tolerance of estrogen-treated rats to acute cold exposure. Female rats treated chronically with ethynylestradiol (36 micrograms/kg per day) alone, and in combination with the progestational agent, norethynodrel (253 micrograms/kg per day), cooled significantly faster than controls when lightly restrained and exposed to air at 5 degrees C. Rate of cooling of rats given only norethynodrel was similar to that of the control group. In other studies, rate of oxygen consumption was determined for all groups during acute exposure to cold (14 degrees C). All estrogen-treated groups achieved the same maximal rate of oxygen consumption as control and norethynodrel-treated groups during cold exposure, but cooled significantly faster. Two groups of female rats were treated chronically with ethynylestradiol at two separate doses (36 and 61 micrograms/kg per day). An untreated group served as controls. Rate of oxygen consumption of all animals were measured during restraint and exposure to cold (18 degrees C). The estrogen-treated groups again achieved the same maximal rate of oxygen consumption as the control group, but also cooled significantly faster despite the fact that the cold stress was less severe than in the previous experiment. That estrogen-treated rats cooled faster than controls in both studies despite achieving a maximal rate of heat production which did not differ from controls suggests that reduced cold tolerance of estrogen-treated rats may be related to increased heat loss."}
{"id": "PMID:468674", "title": "Gas-to-blood PCO2 differences during severe hypercapnia.", "content": "Five anesthetized dogs were made severely hypercapnic by stepwise addition of CO2 to their inspired air. Blood PCO2 levels greater than 400 Torr were reached. During hypercapnia, the steady-state end-tidal PCO2 (PaCO2) was always higher than the simultaneous measured arterial PCO2 (PaCO2). The mean ratio PaCO2/PACO2 was 0.861 +/- 0.01. These results are consistent with the predictions of the Charged Membrane Hypothesis, that gas-to-blood PCO2 differences should be directly proportional to the blood H+ activity. The results cannot be explained by delayed equilibration of CO2 between plasma and red blood cells. The latter hypothesis predicts that, under the conditions of these experiments, the PCO2 of arterial blood should be higher than the PCO2 of end-tibal gas. The blood HCO3- during hypercapnia did not increase as much as would be predicted if the blood were exposed to CO2 in vitro. This may reflect movement of blood HCO3- generated by the buffering of carbonic acid into intracellular compartments during hypercapnia.", "contents": "Gas-to-blood PCO2 differences during severe hypercapnia. Five anesthetized dogs were made severely hypercapnic by stepwise addition of CO2 to their inspired air. Blood PCO2 levels greater than 400 Torr were reached. During hypercapnia, the steady-state end-tidal PCO2 (PaCO2) was always higher than the simultaneous measured arterial PCO2 (PaCO2). The mean ratio PaCO2/PACO2 was 0.861 +/- 0.01. These results are consistent with the predictions of the Charged Membrane Hypothesis, that gas-to-blood PCO2 differences should be directly proportional to the blood H+ activity. The results cannot be explained by delayed equilibration of CO2 between plasma and red blood cells. The latter hypothesis predicts that, under the conditions of these experiments, the PCO2 of arterial blood should be higher than the PCO2 of end-tibal gas. The blood HCO3- during hypercapnia did not increase as much as would be predicted if the blood were exposed to CO2 in vitro. This may reflect movement of blood HCO3- generated by the buffering of carbonic acid into intracellular compartments during hypercapnia."}
{"id": "PMID:468675", "title": "Protein metabolism in lung: use of isolated perfused lung to study protein degradation.", "content": "This study investigates the use of the isolated perfused lung to study protein degradation. Proteins were labeled in vivo for 10 min or for 5 h using L-[U-14C]phenylalanine. When prelabeled lungs were perfused in vitro virtually all of the acid-soluble and acid-insoluble radioactivity in the tissue and perfusate remained as phenylalanine. Protein degradation was measured as the accumulation of free [14C]phenylalanine in ther perfusate; during the time this accumulated the amount of intracellular free phenylalanine and the free phenylalanine space remained constant. Proteins labeled during 10 min had a constant rate of degradation between 45 and 90 min of perfusion (about 11%.h-1); those labeled during 5 h had a constant rate of degradation for 90 (about 3%.h-1). The percent dry lung weight did not change during the perfusion. We conclude that measurable rates of proteolysis of \"rapid\" and \"slowly\" turning over proteins can be obtained while the lung is virtually free of edema. This system should allow studies on the modulation of proteolysis in intact lung under defined conditions.", "contents": "Protein metabolism in lung: use of isolated perfused lung to study protein degradation. This study investigates the use of the isolated perfused lung to study protein degradation. Proteins were labeled in vivo for 10 min or for 5 h using L-[U-14C]phenylalanine. When prelabeled lungs were perfused in vitro virtually all of the acid-soluble and acid-insoluble radioactivity in the tissue and perfusate remained as phenylalanine. Protein degradation was measured as the accumulation of free [14C]phenylalanine in ther perfusate; during the time this accumulated the amount of intracellular free phenylalanine and the free phenylalanine space remained constant. Proteins labeled during 10 min had a constant rate of degradation between 45 and 90 min of perfusion (about 11%.h-1); those labeled during 5 h had a constant rate of degradation for 90 (about 3%.h-1). The percent dry lung weight did not change during the perfusion. We conclude that measurable rates of proteolysis of \"rapid\" and \"slowly\" turning over proteins can be obtained while the lung is virtually free of edema. This system should allow studies on the modulation of proteolysis in intact lung under defined conditions."}
{"id": "PMID:468676", "title": "Degradation of endogenous protein by rabbit pulmonary macrophages.", "content": "Pulmonary macrophages were preincubated for 1 or 20 h with L-[U-14C]phenylalanine and the degradation of labeled proteins studied by reincubating these cells in the presence of 4 mM  L-phenylalanine and measuring rates of [14C]phenylalanine released from the cells into the medium. We found that proteins prelabeled in 1 or 20 h were degraded 8.0 and 3.0%.h-1, respectively. Decreases in cell viability reduced the rate of protein degradation. Lack of exogenous glucose slowed the rate of degradation of proteins labeled in 1 h, but not of those labeled in 20 h. Varying amino acids in the medium from normal to 5 times normal rabbit plasma levels had no effect on the degradation of either group of proteins. Rates of degradation of both fast and slowly turning over proteins were inhibited during phagocytosis of polystyrene latex particles by about 62 and 33%, respectively. The time course of the changes in protein degradation suggests they are related to intracellular events in the phagocytic process, rather than particle attachment or uptake.", "contents": "Degradation of endogenous protein by rabbit pulmonary macrophages. Pulmonary macrophages were preincubated for 1 or 20 h with L-[U-14C]phenylalanine and the degradation of labeled proteins studied by reincubating these cells in the presence of 4 mM  L-phenylalanine and measuring rates of [14C]phenylalanine released from the cells into the medium. We found that proteins prelabeled in 1 or 20 h were degraded 8.0 and 3.0%.h-1, respectively. Decreases in cell viability reduced the rate of protein degradation. Lack of exogenous glucose slowed the rate of degradation of proteins labeled in 1 h, but not of those labeled in 20 h. Varying amino acids in the medium from normal to 5 times normal rabbit plasma levels had no effect on the degradation of either group of proteins. Rates of degradation of both fast and slowly turning over proteins were inhibited during phagocytosis of polystyrene latex particles by about 62 and 33%, respectively. The time course of the changes in protein degradation suggests they are related to intracellular events in the phagocytic process, rather than particle attachment or uptake."}
{"id": "PMID:468677", "title": "Pattern and mechanism of airway response to hypocapnia in normal subjects.", "content": "We examined the bronchoconstriction produced by airway hypocapnia in normal subjects. Maximal expiratory flow at 25% vital capacity on partial expiratory flow-volume (PEFV) curves fell during hypocapnia both on air and on an 80% helium- 20% oxygen mixture. Density dependence also fell, suggesting predominantly small airway constriction. The changes seen on PEFV curves were not found on maximal expiratory flow-volume curves, indicating the inhalation to total lung capacity substantially reversed the constriction. Pretreatment with a beta-sympathomimetic agent blocked the response, whereas atropine pretreatment did not, suggesting that hypocapnia affects airway smooth muscle directly, not via cholinergic efferents.", "contents": "Pattern and mechanism of airway response to hypocapnia in normal subjects. We examined the bronchoconstriction produced by airway hypocapnia in normal subjects. Maximal expiratory flow at 25% vital capacity on partial expiratory flow-volume (PEFV) curves fell during hypocapnia both on air and on an 80% helium- 20% oxygen mixture. Density dependence also fell, suggesting predominantly small airway constriction. The changes seen on PEFV curves were not found on maximal expiratory flow-volume curves, indicating the inhalation to total lung capacity substantially reversed the constriction. Pretreatment with a beta-sympathomimetic agent blocked the response, whereas atropine pretreatment did not, suggesting that hypocapnia affects airway smooth muscle directly, not via cholinergic efferents."}
{"id": "PMID:468678", "title": "Intestinal transport of hexoses in the rat following chronic heat exposure.", "content": "The effect of 3 wk heat exposure (Ta 34 degrees C) on intestinal weight and intestinal absorption of D-glucose and D-galactose in vitro was examined in the rat. Intestinal dry weight was reduced with heat exposure compared to both ad libitum and pair-fed animals at Ta 22 degrees C. Intestinal tissue water was elevated after pair feeding but not heat exposure; extracellular (inulin) space was similar in the three groups. Mucosal uptake of glucose per gram wet weight in an everted sac preparation was unchanged compared to pair-fed animals, but serosal transfer was increased. Intestinal metabolism of glucose was decreased with heat exposure. Galactose accumulation with 30 min incubation was increased in intestinal rings from both heat-exposed and pair-fed animals. This increase is likely to be related to the reduction in ring size present in the groups with reduced food intake. Vmax and apparent Km for galactose transport were unchanged. Our results indicate that despite a reduction in intestinal weight following heat exposure, the ability of the intestine to transport hexoses per gram remains relatively stable. Alterations of hexose transport appear to be related to altered glucose metabolism and not altered transport capacity. Differences in intestinal weight and glucose utilization between pair-fed and heat-exposed animals suggest that the intestinal response to chronic heat exposure is not solely a function at the amount of food consumed. However, the alteration of more than one variable in pair feeding makes interpretation complex.", "contents": "Intestinal transport of hexoses in the rat following chronic heat exposure. The effect of 3 wk heat exposure (Ta 34 degrees C) on intestinal weight and intestinal absorption of D-glucose and D-galactose in vitro was examined in the rat. Intestinal dry weight was reduced with heat exposure compared to both ad libitum and pair-fed animals at Ta 22 degrees C. Intestinal tissue water was elevated after pair feeding but not heat exposure; extracellular (inulin) space was similar in the three groups. Mucosal uptake of glucose per gram wet weight in an everted sac preparation was unchanged compared to pair-fed animals, but serosal transfer was increased. Intestinal metabolism of glucose was decreased with heat exposure. Galactose accumulation with 30 min incubation was increased in intestinal rings from both heat-exposed and pair-fed animals. This increase is likely to be related to the reduction in ring size present in the groups with reduced food intake. Vmax and apparent Km for galactose transport were unchanged. Our results indicate that despite a reduction in intestinal weight following heat exposure, the ability of the intestine to transport hexoses per gram remains relatively stable. Alterations of hexose transport appear to be related to altered glucose metabolism and not altered transport capacity. Differences in intestinal weight and glucose utilization between pair-fed and heat-exposed animals suggest that the intestinal response to chronic heat exposure is not solely a function at the amount of food consumed. However, the alteration of more than one variable in pair feeding makes interpretation complex."}
{"id": "PMID:468679", "title": "Phrenic activity during severe hypercapnia in vagotomized rabbits.", "content": "The central control mechanism of respiratory frequency under varied alveolar carbon dioxide pressure (PACO2 20--200 Torr) was investigated in anesthetized, vagotomized, immobilized, and artificially ventilated rabbits. Central inspiratory activity indicated by phrenic motor discharge was tolerant of the extensive hypercapnia. Under light anesthesia the respiratory frequency (f) decreased in a hyperbolic fashion with increasing PACO2. Under deeper anesthesia or after mesencephalic decerebration the hyperbolic f response to PACO2 was abolished or changed to a hill-type f response (initial increase and subsequent decrease in f) and, on the average, the changes in frequency were much less. We conclude that in the absence of vagal control the respiratory frequency is primarily determined by 1) the periodicity of the bulbopontine inspiratory activity, which is little dependent on PACO2, and 2) a suprapontine acceleratory mechanism, which is depressed by increased PACO2 and highly sensitive to anesthetics. The mechanism of changes in the type of f response to CO2 is discussed.", "contents": "Phrenic activity during severe hypercapnia in vagotomized rabbits. The central control mechanism of respiratory frequency under varied alveolar carbon dioxide pressure (PACO2 20--200 Torr) was investigated in anesthetized, vagotomized, immobilized, and artificially ventilated rabbits. Central inspiratory activity indicated by phrenic motor discharge was tolerant of the extensive hypercapnia. Under light anesthesia the respiratory frequency (f) decreased in a hyperbolic fashion with increasing PACO2. Under deeper anesthesia or after mesencephalic decerebration the hyperbolic f response to PACO2 was abolished or changed to a hill-type f response (initial increase and subsequent decrease in f) and, on the average, the changes in frequency were much less. We conclude that in the absence of vagal control the respiratory frequency is primarily determined by 1) the periodicity of the bulbopontine inspiratory activity, which is little dependent on PACO2, and 2) a suprapontine acceleratory mechanism, which is depressed by increased PACO2 and highly sensitive to anesthetics. The mechanism of changes in the type of f response to CO2 is discussed."}
{"id": "PMID:468680", "title": "Absence of effects of hypoxia on small airway function in humans.", "content": "We measured the effects of sustained isocapnic hypoxia (PAO2 = 40--50 Torr; PACO2 = 38--42 Torr) on tests sensitive to small airway function in healthy human subjects. Maximum expiratory flow-volume curves on air, nitrogen-hypoxic, helium-normoxic, and helium-hypoxic mixtures as well as closing volumes while subjects breathed air and a hypoxic mixture were obtained. We then measured total lung capacity (TLC), both plethysmographically and by inert gas dilution, and used the nonplethysmographic method to measure the effects of hypoxia on TLC. In none of these tests were there any statistically significant changes when values obtained during hypoxia were compared with those during normoxia. It is suggested that previous reports that indicated that TLC was increased by hypoxia might have arisen from a plethysmographic artifact.", "contents": "Absence of effects of hypoxia on small airway function in humans. We measured the effects of sustained isocapnic hypoxia (PAO2 = 40--50 Torr; PACO2 = 38--42 Torr) on tests sensitive to small airway function in healthy human subjects. Maximum expiratory flow-volume curves on air, nitrogen-hypoxic, helium-normoxic, and helium-hypoxic mixtures as well as closing volumes while subjects breathed air and a hypoxic mixture were obtained. We then measured total lung capacity (TLC), both plethysmographically and by inert gas dilution, and used the nonplethysmographic method to measure the effects of hypoxia on TLC. In none of these tests were there any statistically significant changes when values obtained during hypoxia were compared with those during normoxia. It is suggested that previous reports that indicated that TLC was increased by hypoxia might have arisen from a plethysmographic artifact."}
{"id": "PMID:468681", "title": "Cardiac effects of increased lung volume and decreased pleural pressure in man.", "content": "The cardiac effects of increased lung volume and/or decreased intrathoracic pressure were assessed by radionuclide angiography in normal male subjects. Increased lung volume alone produced no change in left ventricular (LV) or right ventricular (RV) end-diastolic size. Decreasing intrathoracic pressure to -30 Torr with a Mueller maneuver led to increases in LV end-diastolic and end-systolic volumes, and to increases in RV diastolic area. LV ejection fraction did not change significantly but RV ejection fraction decreased with the Mueller maneuver. Increases in transmural central venous pressure were also noted with the Mueller maneuver. The effects of combining increased lung volume with the Mueller maneuver were similar to those with the Mueller maneuver alone. These effects are consistent with the hypothesis that producing large negative pleural pressures acts to impede left ventricular outflow (i.e., afterloading) and raises the possibility of similar changes during acute attacks of bronchospasm.", "contents": "Cardiac effects of increased lung volume and decreased pleural pressure in man. The cardiac effects of increased lung volume and/or decreased intrathoracic pressure were assessed by radionuclide angiography in normal male subjects. Increased lung volume alone produced no change in left ventricular (LV) or right ventricular (RV) end-diastolic size. Decreasing intrathoracic pressure to -30 Torr with a Mueller maneuver led to increases in LV end-diastolic and end-systolic volumes, and to increases in RV diastolic area. LV ejection fraction did not change significantly but RV ejection fraction decreased with the Mueller maneuver. Increases in transmural central venous pressure were also noted with the Mueller maneuver. The effects of combining increased lung volume with the Mueller maneuver were similar to those with the Mueller maneuver alone. These effects are consistent with the hypothesis that producing large negative pleural pressures acts to impede left ventricular outflow (i.e., afterloading) and raises the possibility of similar changes during acute attacks of bronchospasm."}
{"id": "PMID:468682", "title": "Mechanical efficiency of fast- and slow-twitch muscle fibers in man during cycling.", "content": "The influence of different percentages of slow-twitch (ST) and fast-twitch (FT) fibers in vastus lateralis on delta efficiency expressed by delta work (x)/delta energy liberation (y) in y = a + bx was studied in six subjects during cycling on an ergometer at 60 or 100 rpm at work loads below 80% of VO2max. Three subjects had an average of 78% ST fibers (ST group) and the other subjects had an average of 76% FT fibers (FT group). There was no difference between the two groups in delta efficiency at 60 rpm, but at 100 rpm the efficiency of the ST group was significantly lower than that of the FT group (19.6 vs. 28.8%, P less than 0.01). In the ST group respiratory exchange ratio (R) was higher at 100 rpm than at 60 rpm, but the FT group had similar R values at both pedal revolution rates. The most important finding was the reduced efficiency when pedaling frequency was increased from 60 to 100 rpm in the ST group (23.3 to 19.6%). Predominant use of ST fibers at rapid pedal rates may require a substantial increase in energy expenditure.", "contents": "Mechanical efficiency of fast- and slow-twitch muscle fibers in man during cycling. The influence of different percentages of slow-twitch (ST) and fast-twitch (FT) fibers in vastus lateralis on delta efficiency expressed by delta work (x)/delta energy liberation (y) in y = a + bx was studied in six subjects during cycling on an ergometer at 60 or 100 rpm at work loads below 80% of VO2max. Three subjects had an average of 78% ST fibers (ST group) and the other subjects had an average of 76% FT fibers (FT group). There was no difference between the two groups in delta efficiency at 60 rpm, but at 100 rpm the efficiency of the ST group was significantly lower than that of the FT group (19.6 vs. 28.8%, P less than 0.01). In the ST group respiratory exchange ratio (R) was higher at 100 rpm than at 60 rpm, but the FT group had similar R values at both pedal revolution rates. The most important finding was the reduced efficiency when pedaling frequency was increased from 60 to 100 rpm in the ST group (23.3 to 19.6%). Predominant use of ST fibers at rapid pedal rates may require a substantial increase in energy expenditure."}
{"id": "PMID:468683", "title": "Frequency dependence of respiratory resistance in healthy children.", "content": "We measured in 130 (61 girls) children aged 3--14 yr respiratory resistance (Rrs), with the oscillation technique, between 4 and 9 Hz. Rrs, at both 4 and 9 Hz, decreased as a function of height (r = 0.74, P less than 0.001). No statistical difference was found between boys and girls. Frequency dependence of resistance (Rrs 4 Hz-Rrs 9 Hz) was found in children at all ages, and decreased with increasing height (r = 0.50, P less than 0.001). We suggest that frequency dependence of resistance in children can be explained on the basis of an increased peripheral resistance, which produces an asynchronous distribution of tidal volume between dead space and lung parenchyma. During growth peripheral resistance decreases and Rrs bcome less frequency dependent to reach at about 15--16 yr independency of frequence.", "contents": "Frequency dependence of respiratory resistance in healthy children. We measured in 130 (61 girls) children aged 3--14 yr respiratory resistance (Rrs), with the oscillation technique, between 4 and 9 Hz. Rrs, at both 4 and 9 Hz, decreased as a function of height (r = 0.74, P less than 0.001). No statistical difference was found between boys and girls. Frequency dependence of resistance (Rrs 4 Hz-Rrs 9 Hz) was found in children at all ages, and decreased with increasing height (r = 0.50, P less than 0.001). We suggest that frequency dependence of resistance in children can be explained on the basis of an increased peripheral resistance, which produces an asynchronous distribution of tidal volume between dead space and lung parenchyma. During growth peripheral resistance decreases and Rrs bcome less frequency dependent to reach at about 15--16 yr independency of frequence."}
{"id": "PMID:468684", "title": "Effect of somatostatin on plasma glucagon and insulin, and glucose turnover in exercising sheep.", "content": "To examine the roles of glucagon and insulin in exercise, four sheep were run on a treadmill with and without simultaneous infusion of somatostatin (SRIF), a peptide that suppresses glucagon and insulin secretion. SRIF infusion suppressed the exercise-induced rise in plasma glucagon during both moderate (5--5.5 km/h) and strenuous exercise (7.0 km/h). In addition, SRIF prevented the rise insulin concentrations during moderate exercise. During strenuous exercise, insulin concentrations were depressed in both groups. The infusion of SRIF was associated with a reduction in exercise-induced glucose production, as determined by infusion of [6-3H]glucose, during the first 15 min of both moderate and strenuous exercise compared to controls. Beyond 15 min glucose production was not significantly altered by SRIF infusions. These data are consistent with glucagon having an immediate, but only transient, stimulatory effect on the exercise-induced hepatic glucose production.", "contents": "Effect of somatostatin on plasma glucagon and insulin, and glucose turnover in exercising sheep. To examine the roles of glucagon and insulin in exercise, four sheep were run on a treadmill with and without simultaneous infusion of somatostatin (SRIF), a peptide that suppresses glucagon and insulin secretion. SRIF infusion suppressed the exercise-induced rise in plasma glucagon during both moderate (5--5.5 km/h) and strenuous exercise (7.0 km/h). In addition, SRIF prevented the rise insulin concentrations during moderate exercise. During strenuous exercise, insulin concentrations were depressed in both groups. The infusion of SRIF was associated with a reduction in exercise-induced glucose production, as determined by infusion of [6-3H]glucose, during the first 15 min of both moderate and strenuous exercise compared to controls. Beyond 15 min glucose production was not significantly altered by SRIF infusions. These data are consistent with glucagon having an immediate, but only transient, stimulatory effect on the exercise-induced hepatic glucose production."}
{"id": "PMID:468685", "title": "Effect of rate of change in skin temperature on local sweating rate.", "content": "To evaluate the relative contributions of positive and negative variations of mean skin temperature (+/- dTsk/dt) on thermoregulatory responses, male resting nude subjects were exposed to rapid or slow alterations in air and wall temperatures (28--45 degrees C; Pa = 20.0 mbar). Rates of heating-cooling cycles were equal to dTa/dt = +/- 3.40, 1.13, 0.57, 0.38, or 0.19 degrees C/min. Continuous measurements were made of rectal, oral, ear, and mean skin temperatures and of arm sweating (dew-point hygrometer method). During all exposures the local skin temperature was kept constant (Tsl = 39 degrees C). The results showed that peripheral inputs are a major factor in thermoregulatory processes. Cutaneous receptors produce a positive and a negative rate component within the central thermal integrator. A higher rate threshold was observed for the positive rate component than for the negative one.", "contents": "Effect of rate of change in skin temperature on local sweating rate. To evaluate the relative contributions of positive and negative variations of mean skin temperature (+/- dTsk/dt) on thermoregulatory responses, male resting nude subjects were exposed to rapid or slow alterations in air and wall temperatures (28--45 degrees C; Pa = 20.0 mbar). Rates of heating-cooling cycles were equal to dTa/dt = +/- 3.40, 1.13, 0.57, 0.38, or 0.19 degrees C/min. Continuous measurements were made of rectal, oral, ear, and mean skin temperatures and of arm sweating (dew-point hygrometer method). During all exposures the local skin temperature was kept constant (Tsl = 39 degrees C). The results showed that peripheral inputs are a major factor in thermoregulatory processes. Cutaneous receptors produce a positive and a negative rate component within the central thermal integrator. A higher rate threshold was observed for the positive rate component than for the negative one."}
{"id": "PMID:468686", "title": "Apnea, tachypnea, and hypotension elicited by cardiac vagal afferents.", "content": "The reflex effects of right and left ventricular distension, mediated by vagal afferents, were studied in mongrel dogs anesthetized with halothane or pentobarbital sodium on heart-lung bypass. Diaphragm electromyogram (D-EMG), systemic blood pressure, and left ventricular cardiogram were all measured during ventricular distension. After bilateral section of the stellate ganglia, distension of the left ventricle produced an apnea, or slowing of respiration and systemic hypotension, without a change in heart rate. A reflex decrease in the amplitude of the D-EMG occurred if the initial breathing rate was high; a decrease in frequency of the D-EMG bursts occurred if the initial rate was low. The left ventricular vagal afferents altering respiration had conduction velocities between 22 and 70 m/s, whereas those causing hypotension had conduction velocities less than 22 m/s. Distension of the right ventricle resulted in a significant tachypnea and systemic hypotension without a change in heart rate. The conduction velocities of the right ventricular vagal afferents causing both tachypnea and hypotension were less than 9 m/s. These reflex changes in respiration and blood pressure elicited by both right and left ventricular distension were eliminated with vagotomy.", "contents": "Apnea, tachypnea, and hypotension elicited by cardiac vagal afferents. The reflex effects of right and left ventricular distension, mediated by vagal afferents, were studied in mongrel dogs anesthetized with halothane or pentobarbital sodium on heart-lung bypass. Diaphragm electromyogram (D-EMG), systemic blood pressure, and left ventricular cardiogram were all measured during ventricular distension. After bilateral section of the stellate ganglia, distension of the left ventricle produced an apnea, or slowing of respiration and systemic hypotension, without a change in heart rate. A reflex decrease in the amplitude of the D-EMG occurred if the initial breathing rate was high; a decrease in frequency of the D-EMG bursts occurred if the initial rate was low. The left ventricular vagal afferents altering respiration had conduction velocities between 22 and 70 m/s, whereas those causing hypotension had conduction velocities less than 22 m/s. Distension of the right ventricle resulted in a significant tachypnea and systemic hypotension without a change in heart rate. The conduction velocities of the right ventricular vagal afferents causing both tachypnea and hypotension were less than 9 m/s. These reflex changes in respiration and blood pressure elicited by both right and left ventricular distension were eliminated with vagotomy."}
{"id": "PMID:468687", "title": "Measurements of the dead space volume.", "content": "The \"anatomical\" dead space is commonly measured by sampling an inert gas (N2) and volume in the exhalation following a large breath of oxygen (VD(F)). It may also be measured from an inert gas washout (VD(O)) that describes both volume and the delivery of VD(O) throughout the expiration. VD(O) is known to increase with age and is enlarged in some obstructive syndromes. VD(O) was appreciably larger than VD(F) in our normal subjects. Both measures increased with lung volume, the increase being entirely due to an increase in the volume of phase I. Physiological dead space (VD(p)) however, did not change significantly with lung volume, showing \"alveolar\" dead space to diminish as a result. An increase in VD(O) occurred with increasing respiratory frequency that was explained by the increase in volume of phase I. Although an increase in VD(F) occurred with frequency, this was significantly less than that seen by VD(O), i.e., VD(F) did not see the progressive increase in phase I volume with frequency. No lung volume or frequency changes, parasympatholytic or sympathomimetic drugs, or altered patterns of breathing simulated the late delivery of dead space seen in age and some obstructive syndromes.", "contents": "Measurements of the dead space volume. The \"anatomical\" dead space is commonly measured by sampling an inert gas (N2) and volume in the exhalation following a large breath of oxygen (VD(F)). It may also be measured from an inert gas washout (VD(O)) that describes both volume and the delivery of VD(O) throughout the expiration. VD(O) is known to increase with age and is enlarged in some obstructive syndromes. VD(O) was appreciably larger than VD(F) in our normal subjects. Both measures increased with lung volume, the increase being entirely due to an increase in the volume of phase I. Physiological dead space (VD(p)) however, did not change significantly with lung volume, showing \"alveolar\" dead space to diminish as a result. An increase in VD(O) occurred with increasing respiratory frequency that was explained by the increase in volume of phase I. Although an increase in VD(F) occurred with frequency, this was significantly less than that seen by VD(O), i.e., VD(F) did not see the progressive increase in phase I volume with frequency. No lung volume or frequency changes, parasympatholytic or sympathomimetic drugs, or altered patterns of breathing simulated the late delivery of dead space seen in age and some obstructive syndromes."}
{"id": "PMID:468688", "title": "In situ perfusion of rat lungs: stability and effects of oxygen tension.", "content": "A new method for perfusion of rat lungs in situ was developed for metabolic studies. The pulmonary circulation was cannulated without contacting the lungs, which remained in the thoracic cage. Perfusion was continued for up to 4 h with Krebs-Henseleit bicarbonate buffer, equilibrated with 95% O2- 5% CO2 and containing 4.5% bovine serum albumin, 5.6 mM glucose, and levels of amino acids normally found in rat plasma. At an arterial pressure of 20 cmH2O flow remained constant (10.9 ml/min.100 g body wt) and appeared evenly distributed among the lobes. Tidal volume was 1 ml/100 g body wt (72/min); positive end-expiratory pressure was 2 cmH2O. The preparation remained stable and metabolically active for 4 h, as evidenced by a minimal decline in dry-to-wet weight ratio, constant levels of ATP and glycogen, a high ratio of glucose uptake to lactate production, and a linear rate of incorporation of [14C]phenylalanine into protein. The lungs were unaffected when perfusate oxygen was reduced to a more physiological level (20% O2-75% N2-5% CO2). In the presence of 95% N2-5% CO2 dry-to-wet weight ratio, ATP, glycogen, and amino acid incorporation decreased, while lactate production doubled.", "contents": "In situ perfusion of rat lungs: stability and effects of oxygen tension. A new method for perfusion of rat lungs in situ was developed for metabolic studies. The pulmonary circulation was cannulated without contacting the lungs, which remained in the thoracic cage. Perfusion was continued for up to 4 h with Krebs-Henseleit bicarbonate buffer, equilibrated with 95% O2- 5% CO2 and containing 4.5% bovine serum albumin, 5.6 mM glucose, and levels of amino acids normally found in rat plasma. At an arterial pressure of 20 cmH2O flow remained constant (10.9 ml/min.100 g body wt) and appeared evenly distributed among the lobes. Tidal volume was 1 ml/100 g body wt (72/min); positive end-expiratory pressure was 2 cmH2O. The preparation remained stable and metabolically active for 4 h, as evidenced by a minimal decline in dry-to-wet weight ratio, constant levels of ATP and glycogen, a high ratio of glucose uptake to lactate production, and a linear rate of incorporation of [14C]phenylalanine into protein. The lungs were unaffected when perfusate oxygen was reduced to a more physiological level (20% O2-75% N2-5% CO2). In the presence of 95% N2-5% CO2 dry-to-wet weight ratio, ATP, glycogen, and amino acid incorporation decreased, while lactate production doubled."}
{"id": "PMID:468689", "title": "Mechanisms of airway smooth muscle response to isoproterenol and theophylline.", "content": "Airway smooth muscle preparations from various sites and species exhibit a range of sensitivities to the same beta-adrenergic agonist. This variability has been attributed to beta-receptor function but the exact mechanism determining the response has not been identified. After first inducing contraction with acetylcholine, we measured isoproterenol and theophylline relaxation responses in five separate airway smooth muscle preparations in vitro. The order of sensitivity was identical for both drugs: guinea pig trachea greater than dog lung strip greater than dog bronchiole greater than rat trachea greater than dog trachea. Because of evidence that both drugs act by increasing adenosine 3',5'-cyclic monophosphate (cAMP) concentrations, we utilized a kinetic model of cAMP metabolism to investigate the possibility that the identical order of sensitivity to both drugs could be explained by a common mechanism. Relaxation responses to both drugs are in accord with known kinetic data. Small differences in the velocity constants of enzymes affecting cAMP metabolism or differences in the relaxation response to the same concentrations of cAMP can fully explain the variable muscle responses to both drugs.", "contents": "Mechanisms of airway smooth muscle response to isoproterenol and theophylline. Airway smooth muscle preparations from various sites and species exhibit a range of sensitivities to the same beta-adrenergic agonist. This variability has been attributed to beta-receptor function but the exact mechanism determining the response has not been identified. After first inducing contraction with acetylcholine, we measured isoproterenol and theophylline relaxation responses in five separate airway smooth muscle preparations in vitro. The order of sensitivity was identical for both drugs: guinea pig trachea greater than dog lung strip greater than dog bronchiole greater than rat trachea greater than dog trachea. Because of evidence that both drugs act by increasing adenosine 3',5'-cyclic monophosphate (cAMP) concentrations, we utilized a kinetic model of cAMP metabolism to investigate the possibility that the identical order of sensitivity to both drugs could be explained by a common mechanism. Relaxation responses to both drugs are in accord with known kinetic data. Small differences in the velocity constants of enzymes affecting cAMP metabolism or differences in the relaxation response to the same concentrations of cAMP can fully explain the variable muscle responses to both drugs."}
{"id": "PMID:468690", "title": "Oxygen affinity of blood in altitude Sherpas.", "content": "Oxygen equilibrium curves on blood within 6 h from sampling have been estimated from polarographic measurements of oxyhemoglobin concentration, in 13 male 14- to 50-yr old Sherpas residing at 3,850 m above sea level (Kumjung, Nepal). In samples with red blood cell counts = 4.7 +/- 0.8 (SD) x 10(6)/mm3, total hemoglobin concentration [Hb] = 17.0 +/- 1.9 g/dl, and hematocrit = 53.3 +/- 5.0, the mean oxygen half-saturation of hemoglobin (P50) (pH = 7.4 and PCO2 = 40 Torr) was 27.3 +/- 1.8 Torr. The P50 of altitude Sherpas was not significantly different from that of acclimatized lowlanders (28.2 +/- 1.3; n = 7), sea-level Caucasian residents (26.5 +/- 1.0; n = 17), and Sherpas at sea level (27.1; n = 3). The 2,3-diphosphoglyceric acid-to-hemoglobin concentration ratio ([2,3-DPG]/[Hb]) in altitude Sherpas was 1.22 +/- 0.03, the same as that of acclimatized Caucasians (1.22 +/- 0.10). The Bohr effect measured for the blood of one altitude Sherpas by the ratio deltalog P50/deltapH was -0.32 and -0.45 at PCO2 levels of 40 and 20 Torr, respectively. These values are not significantly different from those found in Caucasians at sea level where deltalog P50/deltalpH was -0.35 and -0.42, respectively. It is concluded that the P50 in native highlanders is not significantly different from that observed in sea-level dwellers. [2,3-DPG]/[Hb] at altitude, both in natives and in newcomers, is 20% higher than in sea-level residents.", "contents": "Oxygen affinity of blood in altitude Sherpas. Oxygen equilibrium curves on blood within 6 h from sampling have been estimated from polarographic measurements of oxyhemoglobin concentration, in 13 male 14- to 50-yr old Sherpas residing at 3,850 m above sea level (Kumjung, Nepal). In samples with red blood cell counts = 4.7 +/- 0.8 (SD) x 10(6)/mm3, total hemoglobin concentration [Hb] = 17.0 +/- 1.9 g/dl, and hematocrit = 53.3 +/- 5.0, the mean oxygen half-saturation of hemoglobin (P50) (pH = 7.4 and PCO2 = 40 Torr) was 27.3 +/- 1.8 Torr. The P50 of altitude Sherpas was not significantly different from that of acclimatized lowlanders (28.2 +/- 1.3; n = 7), sea-level Caucasian residents (26.5 +/- 1.0; n = 17), and Sherpas at sea level (27.1; n = 3). The 2,3-diphosphoglyceric acid-to-hemoglobin concentration ratio ([2,3-DPG]/[Hb]) in altitude Sherpas was 1.22 +/- 0.03, the same as that of acclimatized Caucasians (1.22 +/- 0.10). The Bohr effect measured for the blood of one altitude Sherpas by the ratio deltalog P50/deltapH was -0.32 and -0.45 at PCO2 levels of 40 and 20 Torr, respectively. These values are not significantly different from those found in Caucasians at sea level where deltalog P50/deltalpH was -0.35 and -0.42, respectively. It is concluded that the P50 in native highlanders is not significantly different from that observed in sea-level dwellers. [2,3-DPG]/[Hb] at altitude, both in natives and in newcomers, is 20% higher than in sea-level residents."}
{"id": "PMID:468691", "title": "Calorigenic effect of insulin in hypothermic dogs.", "content": "In dogs acutely immersed in cold water (8--13 degrees C), oxygen uptake increased approximately sevenfold and colonic temperature rapidly began to decrease. Fifteen minutes after the start of immersion a high level of hyperglycemia was found but no increase in immunoreactive plasma insulin level was observed. Under these conditions exogenous insulin (0.3 U.kg-1) induced a further increase in oxygen uptake and in shivering intensity whereas a decrease in the fall of colonic temperature was observed. It can be concluded that insulin may have a calorigenic effect and improve the resistance to cold of dogs exposed to an acute cold stress.", "contents": "Calorigenic effect of insulin in hypothermic dogs. In dogs acutely immersed in cold water (8--13 degrees C), oxygen uptake increased approximately sevenfold and colonic temperature rapidly began to decrease. Fifteen minutes after the start of immersion a high level of hyperglycemia was found but no increase in immunoreactive plasma insulin level was observed. Under these conditions exogenous insulin (0.3 U.kg-1) induced a further increase in oxygen uptake and in shivering intensity whereas a decrease in the fall of colonic temperature was observed. It can be concluded that insulin may have a calorigenic effect and improve the resistance to cold of dogs exposed to an acute cold stress."}
{"id": "PMID:468692", "title": "Impedance of intrathoracic airway models during low-frequency periodic flow.", "content": "The total pulmonary and lower airway impedances of the normal adult lung were simulated from 0.5 to 10 Hz using a distributed parameter model of the complete tracheobronchial tree. The model includes branching asymmetry; distributed representation of gas compliance, inertance, viscous effects, and inertial distortion of velocity profiles; and nonrigid airway walls. The model predicts closely similar resistance and frequency dependence of resistance but substantially greater reactances than observed by Finucane et al. (J. Appl. Physiol. 38: 517--530, 1975). Increases in resistance with frequency could be explained by changes in the distribution of flow among parallel inhomogeneities (47%), inertial distortion of velocity profiles (35%), changes in the serial distribution of flow due to gas compliance (11%), and airway wall compliance (7%). The disparity between measured and simulated reactance is attirbutable to artifact in the previously reported reactance measurement.", "contents": "Impedance of intrathoracic airway models during low-frequency periodic flow. The total pulmonary and lower airway impedances of the normal adult lung were simulated from 0.5 to 10 Hz using a distributed parameter model of the complete tracheobronchial tree. The model includes branching asymmetry; distributed representation of gas compliance, inertance, viscous effects, and inertial distortion of velocity profiles; and nonrigid airway walls. The model predicts closely similar resistance and frequency dependence of resistance but substantially greater reactances than observed by Finucane et al. (J. Appl. Physiol. 38: 517--530, 1975). Increases in resistance with frequency could be explained by changes in the distribution of flow among parallel inhomogeneities (47%), inertial distortion of velocity profiles (35%), changes in the serial distribution of flow due to gas compliance (11%), and airway wall compliance (7%). The disparity between measured and simulated reactance is attirbutable to artifact in the previously reported reactance measurement."}
{"id": "PMID:468693", "title": "Cardiorespiratory responses following isoproterenol injection in rabbits.", "content": "Receptor sites for the ventilatory response to isoproterenol were investigated in anesthetized rabbits with bolus injections in the common carotid artery (ia) and in the vena cava (iv). The delay from injection to the increase in ventilation (TVE) was significantly shorter following ia (1.5 s) compared to iv injections (about 5 s). The delay to the increase in heart rate (THR) was significantly shorter after iv (about 4.5 s) than after ia injections (12.5 s). When isoproterenol and NaCN injections were compared, there was no difference in TVE. Following carotid body resection, the VE response to isoproterenol was greatly reduced after iv and ia injections; however, THR was unaffected. In intact animals breathing 100% O2 the VE response to isoproterenol was significantly reduced with no change in TVE or in the heart rate response. We conclude that the ventilatory increase following the injection of isoproterenol is due primarily to direct stimulation of the carotid bodies.", "contents": "Cardiorespiratory responses following isoproterenol injection in rabbits. Receptor sites for the ventilatory response to isoproterenol were investigated in anesthetized rabbits with bolus injections in the common carotid artery (ia) and in the vena cava (iv). The delay from injection to the increase in ventilation (TVE) was significantly shorter following ia (1.5 s) compared to iv injections (about 5 s). The delay to the increase in heart rate (THR) was significantly shorter after iv (about 4.5 s) than after ia injections (12.5 s). When isoproterenol and NaCN injections were compared, there was no difference in TVE. Following carotid body resection, the VE response to isoproterenol was greatly reduced after iv and ia injections; however, THR was unaffected. In intact animals breathing 100% O2 the VE response to isoproterenol was significantly reduced with no change in TVE or in the heart rate response. We conclude that the ventilatory increase following the injection of isoproterenol is due primarily to direct stimulation of the carotid bodies."}
{"id": "PMID:468694", "title": "Rheological properties of excised rabbit lung stiffened by repeated hyperinflation.", "content": "Two rheological properties, stress adpatation (S) and hysteresis loop area (H), were studied in normal fresh rabbit lungs and in lungs stiffened by 3 hr repeated inflation at zero end-expiratory pressure. S was measured on the deflation limb at various volume levels ranging from 100 to 25% total lung capacity (TLC). H was obtained in another group of lungs before and after stiffening, for tidal volumes ranging from 5 to 30% TLC, at end-expiratory levels from 25 to 70% TLC. In stiff lung, S after deflation from TLC was biphasic (initial pressure rise, then a fall) whenever the starting pressure exceeded approximately 10 cmH2O. At low lung volumes (25% TLC) stress rose monotonically toward 10 cmH2O. Only monontonic adaptation has previously been reported for normal lung. Tidal H in stiff lungs was increased at all volume levels, occupying roughly 20% of a bounding pressure-volume rectangle, resembling that of normal lungs near TLC but twice that of normal loops at lower volumes. The results suggest that above an equilibrium or transition pressure around 10 cmH2O the alveolar lining in stiff lung may have film properties more resembling those of a viscous liquid, becoming solidlike below this pressure. Similar viscous liquid properties appear to exist on the inflation limb of normal lungs, and near TLC on their deflation limb.", "contents": "Rheological properties of excised rabbit lung stiffened by repeated hyperinflation. Two rheological properties, stress adpatation (S) and hysteresis loop area (H), were studied in normal fresh rabbit lungs and in lungs stiffened by 3 hr repeated inflation at zero end-expiratory pressure. S was measured on the deflation limb at various volume levels ranging from 100 to 25% total lung capacity (TLC). H was obtained in another group of lungs before and after stiffening, for tidal volumes ranging from 5 to 30% TLC, at end-expiratory levels from 25 to 70% TLC. In stiff lung, S after deflation from TLC was biphasic (initial pressure rise, then a fall) whenever the starting pressure exceeded approximately 10 cmH2O. At low lung volumes (25% TLC) stress rose monotonically toward 10 cmH2O. Only monontonic adaptation has previously been reported for normal lung. Tidal H in stiff lungs was increased at all volume levels, occupying roughly 20% of a bounding pressure-volume rectangle, resembling that of normal lungs near TLC but twice that of normal loops at lower volumes. The results suggest that above an equilibrium or transition pressure around 10 cmH2O the alveolar lining in stiff lung may have film properties more resembling those of a viscous liquid, becoming solidlike below this pressure. Similar viscous liquid properties appear to exist on the inflation limb of normal lungs, and near TLC on their deflation limb."}
{"id": "PMID:468695", "title": "Capillary recruitment during airway hypoxia: role of pulmonary artery pressure.", "content": "Hypoxia has been shown to cause an increased number of pulmonary capillaries to be perfused. Changes in cardiac output and left atrial pressure have been previously ruled out as causes of this capillary recruitment. Increased pulmonary vein pressure and increased pulmonary artery pressure remain as two potential mechanisms. To differentiate between these two possible causes, we measured pulmonary artery and vein pressures with directly placed catheters and capillary recruitment with in vivo microscopy. During isocapnic hypoxia pulmonary artery pressure doubled, observed capillary recruitment increased fivefold, and pulmonary vein pressure remained constant. When the vasodilator prostaglandin E1 was infused during hypoxia, pulmonary artery pressure and capillary recruitment fell to control values and pulmonary vein pressure remained constant. Since capillary recruitment correlated with pulmonary artery pressure in each dog, but not with pulmonary vein pressure, we conclude that arterial, not venous, constriction is the probable cause of this recruitment.", "contents": "Capillary recruitment during airway hypoxia: role of pulmonary artery pressure. Hypoxia has been shown to cause an increased number of pulmonary capillaries to be perfused. Changes in cardiac output and left atrial pressure have been previously ruled out as causes of this capillary recruitment. Increased pulmonary vein pressure and increased pulmonary artery pressure remain as two potential mechanisms. To differentiate between these two possible causes, we measured pulmonary artery and vein pressures with directly placed catheters and capillary recruitment with in vivo microscopy. During isocapnic hypoxia pulmonary artery pressure doubled, observed capillary recruitment increased fivefold, and pulmonary vein pressure remained constant. When the vasodilator prostaglandin E1 was infused during hypoxia, pulmonary artery pressure and capillary recruitment fell to control values and pulmonary vein pressure remained constant. Since capillary recruitment correlated with pulmonary artery pressure in each dog, but not with pulmonary vein pressure, we conclude that arterial, not venous, constriction is the probable cause of this recruitment."}
{"id": "PMID:468696", "title": "Torque-velocity relationships and muscle fiber composition in elite female athletes.", "content": "The relationship between the predominance of fast and slow muscle fibers of the vastus lateralis and \"in vivo\" torque velocity properties in 22 female athletes was studied. Fiber types were classified according to the histochemical myofibrillar adenosine triphosphatase technique at a basic pH. Maximal extensor troques were recorded at 30 degrees from full extension at four selected velocities. While results confirm earlier reports on muscle fiber type and performance, an additional finding was that as knee extension velocities increased from 0 to 95 degrees/s angle specific extensor torque production did not decline as seen in in vitro muscle preparations. The difference in extensor torque between 0 and 96 degrees/s appeared far more critical than the differences observed between 96 and 288 degrees/s. Significant differences in torque were seen at 96, 192, and 288 degrees/s in thos with greater than 50% and less than 50% slow-twitch fibers. When expressed per kilogram of body weight the subjects with greater than 50% fast-twitch fiber produced the greatest torque at 192 degrees/s. These results suggest that the velocity at which torque begins to decline in vivo is related to the proportion of slow-twitch fibers in the vastus lateralismuscle.", "contents": "Torque-velocity relationships and muscle fiber composition in elite female athletes. The relationship between the predominance of fast and slow muscle fibers of the vastus lateralis and \"in vivo\" torque velocity properties in 22 female athletes was studied. Fiber types were classified according to the histochemical myofibrillar adenosine triphosphatase technique at a basic pH. Maximal extensor troques were recorded at 30 degrees from full extension at four selected velocities. While results confirm earlier reports on muscle fiber type and performance, an additional finding was that as knee extension velocities increased from 0 to 95 degrees/s angle specific extensor torque production did not decline as seen in in vitro muscle preparations. The difference in extensor torque between 0 and 96 degrees/s appeared far more critical than the differences observed between 96 and 288 degrees/s. Significant differences in torque were seen at 96, 192, and 288 degrees/s in thos with greater than 50% and less than 50% slow-twitch fibers. When expressed per kilogram of body weight the subjects with greater than 50% fast-twitch fiber produced the greatest torque at 192 degrees/s. These results suggest that the velocity at which torque begins to decline in vivo is related to the proportion of slow-twitch fibers in the vastus lateralismuscle."}
{"id": "PMID:468697", "title": "Cage size and exercise affects infarct size in rat after coronary artery cauterization.", "content": "Left coronary occlusion in the rat was performed by cornary artery cauterization. A small amount of myocardial damage at the site of occlusion was noted, and myocardial infarction occurred in the distal distribution of the obstructed coronary. The effects of cage size and level of physical activity on estimated infarct size (as measured by creatine kinase depletion) 48 h after occlusion were determined. Isolation in small cages and moderate treadmill exercise resulted in an approximate doubling of the amount of infarction when compared to grouping of rats in large cages. Mild exercise did not increase infarct size. Total urinary catecholamines in normal rats placed in small cages for 48 h were elevated when compared to unconfined rats in larger cages. Cornary artery occlusion by cauterization is an easily performed technique. Cage size and level of activity (and their effects on sympathoadrenal function) are important independent determinations of infarct extent after coronary occlusion in the rat.", "contents": "Cage size and exercise affects infarct size in rat after coronary artery cauterization. Left coronary occlusion in the rat was performed by cornary artery cauterization. A small amount of myocardial damage at the site of occlusion was noted, and myocardial infarction occurred in the distal distribution of the obstructed coronary. The effects of cage size and level of physical activity on estimated infarct size (as measured by creatine kinase depletion) 48 h after occlusion were determined. Isolation in small cages and moderate treadmill exercise resulted in an approximate doubling of the amount of infarction when compared to grouping of rats in large cages. Mild exercise did not increase infarct size. Total urinary catecholamines in normal rats placed in small cages for 48 h were elevated when compared to unconfined rats in larger cages. Cornary artery occlusion by cauterization is an easily performed technique. Cage size and level of activity (and their effects on sympathoadrenal function) are important independent determinations of infarct extent after coronary occlusion in the rat."}
{"id": "PMID:468698", "title": "Effect of catecholamines on ion transport in dog tracheal epithelium.", "content": "The action of adrenergic agonists on ion fluxes across the epithelium of the canine trachea was determined. Isolated sheets of tracheal mucosal membranes were mounted in Ussing-type chambers, bathed with Krebs-Henseleit solution at 37 degrees C, pH 7.4, and gassed with 5% CO2 Iin oxygen. Various adrenergic agents, when added to the bathing medium elevated short-circuit current (SCC) (isoproterenol greater than epinephrine greater than norepinephrine greater than phenylephrine). Propranolol (10(-6) M) decreased SCC response to epinephrine. Epinephrine (1mM) increased both unidirectional 36Cl fluxes; net 36Cl secretion toward the lumen increased from 2.01 +/- 0.52 to 3.20 +/- 0.46 mueg/cm2.h (P less than 0.05); 22Na flux did not change. In epinephrine-stimulated tissues, propranolol (1mM) abolished net 36Cl secretion. The SCC response to epinephrine was blunted in the absence of Na from the submucosal reservoir; this blunting suggests that Cl entry across the basal membrane is coupled with Na. Both the relative sensitivities of SCC to various adrenergic agonists and the effect of propranolol suggest that Cl-secretory process is highly sensitive to beta-adrenergic receptor stimulation. Epinephrine increased cell membrane permeability to Cl and probably stimulated a specific Cl pump.", "contents": "Effect of catecholamines on ion transport in dog tracheal epithelium. The action of adrenergic agonists on ion fluxes across the epithelium of the canine trachea was determined. Isolated sheets of tracheal mucosal membranes were mounted in Ussing-type chambers, bathed with Krebs-Henseleit solution at 37 degrees C, pH 7.4, and gassed with 5% CO2 Iin oxygen. Various adrenergic agents, when added to the bathing medium elevated short-circuit current (SCC) (isoproterenol greater than epinephrine greater than norepinephrine greater than phenylephrine). Propranolol (10(-6) M) decreased SCC response to epinephrine. Epinephrine (1mM) increased both unidirectional 36Cl fluxes; net 36Cl secretion toward the lumen increased from 2.01 +/- 0.52 to 3.20 +/- 0.46 mueg/cm2.h (P less than 0.05); 22Na flux did not change. In epinephrine-stimulated tissues, propranolol (1mM) abolished net 36Cl secretion. The SCC response to epinephrine was blunted in the absence of Na from the submucosal reservoir; this blunting suggests that Cl entry across the basal membrane is coupled with Na. Both the relative sensitivities of SCC to various adrenergic agonists and the effect of propranolol suggest that Cl-secretory process is highly sensitive to beta-adrenergic receptor stimulation. Epinephrine increased cell membrane permeability to Cl and probably stimulated a specific Cl pump."}
{"id": "PMID:468699", "title": "Cross-adaptive effects of cold, hypoxia, or physical training on decompression sickness in mice.", "content": "The effects of adaptation to cold, hypoxia, or exercise on hyperbaric decompression tolerance were investigated in two factorial experiments. For either 14 or 28 days, groups of mice were handled (control); exposed discontinuously for 4 h to cold (4 degrees C) or hypoxia (P approximately 379 or 320 Torr); or exercised by swimming (15 min at 31 degrees C) or treadmill excursion (8.1 m/min for 1 or 1.5 h). The animals were divided into subgroups, exposed to one of three hydrostatic pressures (7.6--11.1 ATA) for 30 min, decompressed, and observed to determine survival rate or bends incidence (type II decompression sickness). Decompression sickness was significantly reduced (P less than 0.05) in the treadmill-trained animals, was unchanged in cold-exposed and swim-exercised mice, and tended to increase in animals adapted to hypoxia. Enhanced tolerance by treadmill training is presumably due to lean body conformation, which could reduce nitrogen saturation of tissues, and greater muscle capillarization and cardiovascular fitness, which may improve nitrogen elimination. Reduced tolerance with adaptation to hypoxia may be attributed to rheological changes associated with polycythemia, which facilitate bubble production.", "contents": "Cross-adaptive effects of cold, hypoxia, or physical training on decompression sickness in mice. The effects of adaptation to cold, hypoxia, or exercise on hyperbaric decompression tolerance were investigated in two factorial experiments. For either 14 or 28 days, groups of mice were handled (control); exposed discontinuously for 4 h to cold (4 degrees C) or hypoxia (P approximately 379 or 320 Torr); or exercised by swimming (15 min at 31 degrees C) or treadmill excursion (8.1 m/min for 1 or 1.5 h). The animals were divided into subgroups, exposed to one of three hydrostatic pressures (7.6--11.1 ATA) for 30 min, decompressed, and observed to determine survival rate or bends incidence (type II decompression sickness). Decompression sickness was significantly reduced (P less than 0.05) in the treadmill-trained animals, was unchanged in cold-exposed and swim-exercised mice, and tended to increase in animals adapted to hypoxia. Enhanced tolerance by treadmill training is presumably due to lean body conformation, which could reduce nitrogen saturation of tissues, and greater muscle capillarization and cardiovascular fitness, which may improve nitrogen elimination. Reduced tolerance with adaptation to hypoxia may be attributed to rheological changes associated with polycythemia, which facilitate bubble production."}
{"id": "PMID:468700", "title": "Prolonged methacholine-induced bronchoconstriction in dogs.", "content": "We studied the effect of prolonged airways obstruction induced by extended cholinergic stimulation in five anesthetized, mechanically ventilated dogs. A continuous intravenous metacholine infusion was utilized to maintain pulmonary resistance (RL) at 200--1500% preinfusion levels for 13--23 h. At maximum RL (18.86 +/- 7.74 vs. 2.09 +/- 0.18 (mean +/- SD) cmH2O/ (L/S) PREINfusion; P less than 0.01), dynamic lung compliance (Cdyn) fell from 67.5 +/- 14.6 to 32.7 +/- 11.6 ml/cmH2O (P less than 0.005) and arterial partial pressure of oxygen (PaO2) fell modestly from 95.8 +/- 6.1 Torr preinfusion to 83.2 +/- 12.7 Torr (P less than 0.05). Tachyphylaxis to methacholine developed, requiring increases in infusion rates to maintain elevated RL. Abnormalities in lung function resolved promptly upon termination of the infusion. Two similarly instrumented control animals ventilated for 19 and 25 h without metacholine infusion had no change in RL, Cdyn, or PaO2. Histological examination of the lungs revealed no differences between infused and control animals. In spite of marked increases in RL, prolonged cholinergic stimulation produced only mild changes in gas exchange and no sustained changes in lung function or structure.", "contents": "Prolonged methacholine-induced bronchoconstriction in dogs. We studied the effect of prolonged airways obstruction induced by extended cholinergic stimulation in five anesthetized, mechanically ventilated dogs. A continuous intravenous metacholine infusion was utilized to maintain pulmonary resistance (RL) at 200--1500% preinfusion levels for 13--23 h. At maximum RL (18.86 +/- 7.74 vs. 2.09 +/- 0.18 (mean +/- SD) cmH2O/ (L/S) PREINfusion; P less than 0.01), dynamic lung compliance (Cdyn) fell from 67.5 +/- 14.6 to 32.7 +/- 11.6 ml/cmH2O (P less than 0.005) and arterial partial pressure of oxygen (PaO2) fell modestly from 95.8 +/- 6.1 Torr preinfusion to 83.2 +/- 12.7 Torr (P less than 0.05). Tachyphylaxis to methacholine developed, requiring increases in infusion rates to maintain elevated RL. Abnormalities in lung function resolved promptly upon termination of the infusion. Two similarly instrumented control animals ventilated for 19 and 25 h without metacholine infusion had no change in RL, Cdyn, or PaO2. Histological examination of the lungs revealed no differences between infused and control animals. In spite of marked increases in RL, prolonged cholinergic stimulation produced only mild changes in gas exchange and no sustained changes in lung function or structure."}
{"id": "PMID:468701", "title": "Cardiac function in goats exposed to carbon monoxide.", "content": "To investigate the possibility that prolonged carbon monoxide (CO) exposure would depress myocardial function, six chronically instrumented, unsedated goats were exposed to 160--200 ppm CO for 2 wk, resulting in a mean carboxyhemoglobin saturation of 20%. Cardiac index and stroke volume remained unchanged during and after exposure. Hematocrit and hemoglobin concentration started increasing on the 10th day of exposure, this increase reached statistical significance (P less than 0.05) on the 6th postexposure day. Contractility (Vmax) of the left ventricular myocardium and heart rate were unchanged during exposure to CO, but both were significantly (P less than 0.05) decreased at some time during the 1st wk after removal from CO. If there was a decrease in intrinsic myocardial function during CO exposure, it may have been masked by increased sympathetic activity. The mechanism(s) that might produce the decrease in heart rate and contractility after removal from CO are not obvious. Possible explanations are discussed.", "contents": "Cardiac function in goats exposed to carbon monoxide. To investigate the possibility that prolonged carbon monoxide (CO) exposure would depress myocardial function, six chronically instrumented, unsedated goats were exposed to 160--200 ppm CO for 2 wk, resulting in a mean carboxyhemoglobin saturation of 20%. Cardiac index and stroke volume remained unchanged during and after exposure. Hematocrit and hemoglobin concentration started increasing on the 10th day of exposure, this increase reached statistical significance (P less than 0.05) on the 6th postexposure day. Contractility (Vmax) of the left ventricular myocardium and heart rate were unchanged during exposure to CO, but both were significantly (P less than 0.05) decreased at some time during the 1st wk after removal from CO. If there was a decrease in intrinsic myocardial function during CO exposure, it may have been masked by increased sympathetic activity. The mechanism(s) that might produce the decrease in heart rate and contractility after removal from CO are not obvious. Possible explanations are discussed."}
{"id": "PMID:468702", "title": "Recovery of skeletal muscle after 3 mo of hindlimb immobilization in rats.", "content": "During immobilization, skeletal muscle undergoes decreases in size and strength with concomitant atrophic and degenerative changes in slow-twitch muscle fibers. Currently there are no objective data in slow-twitch muscle demonstrating recovery of biochemical or physiological indices following termination of immobilization. The purpose of this study was to determine whether the soleus, a slow-twitch muscle, could recover normal biochemical or physiological levels following termination of immobliization. Adenosine triphosphate, glycogen, and protein concentration (mg/g wet wt) all significantly decreased following 90 days of hindlimb immobilization, but these three values returned to control levels by the 60th recovery day. Similarly, soleus muscle wet weight and protein content (mg protein/muscle) returned to control levels by the 14th recovery day. In contrast, maximal isometric tension did not return to normal until the 120th day. These results indicate that following muscular atrophy, which was achieved through 90 days of hindlimb immoblization, several biochemical and physiological values in skeletal muscle are recovered at various times after the end of immobilization.", "contents": "Recovery of skeletal muscle after 3 mo of hindlimb immobilization in rats. During immobilization, skeletal muscle undergoes decreases in size and strength with concomitant atrophic and degenerative changes in slow-twitch muscle fibers. Currently there are no objective data in slow-twitch muscle demonstrating recovery of biochemical or physiological indices following termination of immobilization. The purpose of this study was to determine whether the soleus, a slow-twitch muscle, could recover normal biochemical or physiological levels following termination of immobliization. Adenosine triphosphate, glycogen, and protein concentration (mg/g wet wt) all significantly decreased following 90 days of hindlimb immobilization, but these three values returned to control levels by the 60th recovery day. Similarly, soleus muscle wet weight and protein content (mg protein/muscle) returned to control levels by the 14th recovery day. In contrast, maximal isometric tension did not return to normal until the 120th day. These results indicate that following muscular atrophy, which was achieved through 90 days of hindlimb immoblization, several biochemical and physiological values in skeletal muscle are recovered at various times after the end of immobilization."}
{"id": "PMID:468703", "title": "Isocapnic hyperpnea in awake ponies during inspiration of 4% CO2.", "content": "Unanesthetized ponies were given 4% CO2 (inspired CO2 pressure = 28 Torr) to breathe at two levels of arterial O2 pressure (PaO2): 1) near 75 Torr and 2) near 200 Torr. During 4% CO2 breathing, at either level of PaO2, the mean arterial CO2 pressure (PaCO2) was unchanged from control measurements (control measurements were made at the same PaO2, but with no CO2 in inspired air), suggesting that awake ponies can \"clear\" 4% CO2. The ability of individual ponies to clear 4% CO2 was quite variable: some ponies did not clear 4% CO2 and others cleared 4% CO2 on one day but not on the following day. Based on the average of 20 experiments, however, PaCO2 was unchanged from 40 Torr during inspiration of 4% CO2. Direct measurement of chemical stimuli to breathing in arterial blood and cisternal cerebrospinal fluid indicate that ventilation increased during CO2 breathing even though PaCO2, pHa, PaO2, and CSF pH were not changed in a direction that might explain the accompanying change in ventilation. The authors suggest that stimuli to receptors other than peripheral or medullary chemoreceptors may be responsible for the reported \"isocapnic hyperpnea.\"", "contents": "Isocapnic hyperpnea in awake ponies during inspiration of 4% CO2. Unanesthetized ponies were given 4% CO2 (inspired CO2 pressure = 28 Torr) to breathe at two levels of arterial O2 pressure (PaO2): 1) near 75 Torr and 2) near 200 Torr. During 4% CO2 breathing, at either level of PaO2, the mean arterial CO2 pressure (PaCO2) was unchanged from control measurements (control measurements were made at the same PaO2, but with no CO2 in inspired air), suggesting that awake ponies can \"clear\" 4% CO2. The ability of individual ponies to clear 4% CO2 was quite variable: some ponies did not clear 4% CO2 and others cleared 4% CO2 on one day but not on the following day. Based on the average of 20 experiments, however, PaCO2 was unchanged from 40 Torr during inspiration of 4% CO2. Direct measurement of chemical stimuli to breathing in arterial blood and cisternal cerebrospinal fluid indicate that ventilation increased during CO2 breathing even though PaCO2, pHa, PaO2, and CSF pH were not changed in a direction that might explain the accompanying change in ventilation. The authors suggest that stimuli to receptors other than peripheral or medullary chemoreceptors may be responsible for the reported \"isocapnic hyperpnea.\""}
{"id": "PMID:468704", "title": "A technique for measuring frequency response of pressure, volume, and flow transducers.", "content": "A device and methodology is presented for testing the frequency response of pressure, volume, or flow transducers. Also reported are responses of selected transducers of all three types over the range of 2--120 Hz. Several pressure transducers tested had good frequency response when connected to the test system with a minimum of interconnecting fittings; others did not. Use of additional connectors degraded the response as did the addition of air-filled catheters. The frequency response of the pneumotachometers tested were influenced largely by the response characteristics of the associated pressure transducer and interconnecting fittings. These results emphasize the need to test the response characteristics of any transducer with specific connectors and fittings that are to be used to make the actual measurements of pressure, volume, or flow.", "contents": "A technique for measuring frequency response of pressure, volume, and flow transducers. A device and methodology is presented for testing the frequency response of pressure, volume, or flow transducers. Also reported are responses of selected transducers of all three types over the range of 2--120 Hz. Several pressure transducers tested had good frequency response when connected to the test system with a minimum of interconnecting fittings; others did not. Use of additional connectors degraded the response as did the addition of air-filled catheters. The frequency response of the pneumotachometers tested were influenced largely by the response characteristics of the associated pressure transducer and interconnecting fittings. These results emphasize the need to test the response characteristics of any transducer with specific connectors and fittings that are to be used to make the actual measurements of pressure, volume, or flow."}
{"id": "PMID:468705", "title": "Light and electron microscopic observations on the development of the blood vascular system of the human brain.", "content": "The development of the blood vascular system of the human fetal brain was examined by both light and electron microscopy. By light microscopy the brains of human embryos and fetuses ranging in size from 8.5 to 70 mm crown-rump length have been studied in serial sections, usually of the whole embryo or fetus. In the smallest specimens the whole brain was encapsulated by a very dense vascular plexus. From this, perforating offshoots passed to or from the substance of the brain, while other connexions were effected with neighbouring arterial and venous channels. These latter vessels were never more mature than capillaries, and their status only recognizable by their location and ultimately by their connexions with the heart. The neopallial part of the cerebral hemisphere was later than all other parts of the brain in receiving vessels perforating its substances. There is some evidence, however, that the cellular basis of a blood vascular supply was present in this part of the brain before lumina and blood cells appeared. The cerebral cortex of fetuses ranging from 50 to 100 mm crown-rump length was examined. Both the extrinsic and intrinsic vessels of the cortex were never more mature than capillaries; occasionally a capillary was seen to penetrate the cortex from the surrounding pial investment. Within the developing cerebral cortex blind ending solid endothelial sprouts were identified, as well as 'seamless' capillaries.", "contents": "Light and electron microscopic observations on the development of the blood vascular system of the human brain. The development of the blood vascular system of the human fetal brain was examined by both light and electron microscopy. By light microscopy the brains of human embryos and fetuses ranging in size from 8.5 to 70 mm crown-rump length have been studied in serial sections, usually of the whole embryo or fetus. In the smallest specimens the whole brain was encapsulated by a very dense vascular plexus. From this, perforating offshoots passed to or from the substance of the brain, while other connexions were effected with neighbouring arterial and venous channels. These latter vessels were never more mature than capillaries, and their status only recognizable by their location and ultimately by their connexions with the heart. The neopallial part of the cerebral hemisphere was later than all other parts of the brain in receiving vessels perforating its substances. There is some evidence, however, that the cellular basis of a blood vascular supply was present in this part of the brain before lumina and blood cells appeared. The cerebral cortex of fetuses ranging from 50 to 100 mm crown-rump length was examined. Both the extrinsic and intrinsic vessels of the cortex were never more mature than capillaries; occasionally a capillary was seen to penetrate the cortex from the surrounding pial investment. Within the developing cerebral cortex blind ending solid endothelial sprouts were identified, as well as 'seamless' capillaries."}
{"id": "PMID:468706", "title": "Development of mucosa of the human ileum.", "content": "Normal histology, glycogen and alkaline phosphatase have been studied in the mucosa of human fetal ileum between 28 and 140 days of gestation. From 112 days onwards, the perinuclear, supranuclear and infranuclear vacuolation, striate border, and basement membrane of the columnar epithelial cells of the villi, and the very close proximity of the latter to blood vessels, all support the idea that the cells are actively engaged in transport of materials. Alkaline phosphatase and glycogen become conspicuous in the cells at this stage. It is argued that by 112 days the epithelial cells of the ileum are absorbing glucose amniotic fluid as well as secreting mucus.", "contents": "Development of mucosa of the human ileum. Normal histology, glycogen and alkaline phosphatase have been studied in the mucosa of human fetal ileum between 28 and 140 days of gestation. From 112 days onwards, the perinuclear, supranuclear and infranuclear vacuolation, striate border, and basement membrane of the columnar epithelial cells of the villi, and the very close proximity of the latter to blood vessels, all support the idea that the cells are actively engaged in transport of materials. Alkaline phosphatase and glycogen become conspicuous in the cells at this stage. It is argued that by 112 days the epithelial cells of the ileum are absorbing glucose amniotic fluid as well as secreting mucus."}
{"id": "PMID:468707", "title": "Myotube formation in skeletal muscle regeneration.", "content": "Muscle fibre regeneration in the latissimus dorsi of adult rats has been studied by light and electron microscopy. Both 'continuous' and 'discontinuous' modes of regeneration occur. In 'continuous' regeneration sarcoplasmic buds grow from the damaged muscle fibres, their nuclei apparently originating from the healthy portion of the muscle fibre. In 'discontinuous' regeneration mononucleated cells accumulate inside intact but vacated, sarcolemmal tubes. They differentiate into myofibril-containing myoblasts which later fuse. 'Continuous' regeneration occurs when the sarcolemmal sheaths are disrupted; 'discontinuous' regeneration when the sarcolemmal sheaths remain intact.", "contents": "Myotube formation in skeletal muscle regeneration. Muscle fibre regeneration in the latissimus dorsi of adult rats has been studied by light and electron microscopy. Both 'continuous' and 'discontinuous' modes of regeneration occur. In 'continuous' regeneration sarcoplasmic buds grow from the damaged muscle fibres, their nuclei apparently originating from the healthy portion of the muscle fibre. In 'discontinuous' regeneration mononucleated cells accumulate inside intact but vacated, sarcolemmal tubes. They differentiate into myofibril-containing myoblasts which later fuse. 'Continuous' regeneration occurs when the sarcolemmal sheaths are disrupted; 'discontinuous' regeneration when the sarcolemmal sheaths remain intact."}
{"id": "PMID:468708", "title": "The anterior fibres of the levator ani muscle in man.", "content": "The anatomy of the anterior portion of the levator ani muscle in studied in 26 adult human cadavers of both sexes. This portion of the muscle is found to consist of three layers of muscle fibres. The three layers are: 1. The pelvic layer. Its fibres (1) are attached to the capsule of the prostate or adventitia of the lateral wall of vagina, (2) intermingle with and supplement the longitudinal muscle layer of the anal canal, and (3) are continuous with the fibres of the opposite side behind the recto-anal junction. 2. The middle layer. The most anterior fibres are twisted on themselves to form the round free border of the muscle that bounds the levator hiatus. The majority of the muscle fibres of this layer proceed backwards to cover and blend with the deep part of the external anal shincter, partly joining the anococcygeal ligament. 3. The perineal layer. These fibres surround the superficial part of the external anal sphincter. A respectable bundle of muscles fibres unites with that of the opposite side in from  of the lower part of the anal canal. Remaining fibres terminate in perianal skin or anococcygeal ligament. The role of the anterior portion of the levator ani in fixation and prevention of prolapse of the pelvic viscera is stressed.", "contents": "The anterior fibres of the levator ani muscle in man. The anatomy of the anterior portion of the levator ani muscle in studied in 26 adult human cadavers of both sexes. This portion of the muscle is found to consist of three layers of muscle fibres. The three layers are: 1. The pelvic layer. Its fibres (1) are attached to the capsule of the prostate or adventitia of the lateral wall of vagina, (2) intermingle with and supplement the longitudinal muscle layer of the anal canal, and (3) are continuous with the fibres of the opposite side behind the recto-anal junction. 2. The middle layer. The most anterior fibres are twisted on themselves to form the round free border of the muscle that bounds the levator hiatus. The majority of the muscle fibres of this layer proceed backwards to cover and blend with the deep part of the external anal shincter, partly joining the anococcygeal ligament. 3. The perineal layer. These fibres surround the superficial part of the external anal sphincter. A respectable bundle of muscles fibres unites with that of the opposite side in from  of the lower part of the anal canal. Remaining fibres terminate in perianal skin or anococcygeal ligament. The role of the anterior portion of the levator ani in fixation and prevention of prolapse of the pelvic viscera is stressed."}
{"id": "PMID:468709", "title": "Inguinal anatomy.", "content": "The flat muscles of the anterior abdominal wall pass down well in front of the pectineal ligament of Astley Cooper on the summit of the superior pubic rumus, thus providing space for the spermatic cord and the great vessels of the lower limb to leave the abdomen. The abdominal wall layers turn back lower down to close the gap on the medial side of the external iliac vein. The external oblique aponeurosis turns back to form a strong inguinal ligament, and the attached fascia lata turns back as the lacunar ligament to meet and fuse with the pectineus muscle fascia 1-1.5 cm below Cooper's ligament. This leaves a deep trough, between Cooper's ligament behind and the posterior inguinal wall in front, which houses the transversely placed femoral canal. The transversalis fascia of the floor of the femoral canal turns down to form the medial wall of the venous compartment of the femoral sheath, and has the support of the curved edge of the lacunar ligament which effectively bars the femoral canal from entering the thigh. It is usually taught, however, that the lower end of the femoral canal passes unprotected into the thigh as the medial or third compartment of the femoral sheath, to lie between the femoral vein in its sheath and the curved edge of the lacunar ligament. If this were the case there would be no hindrance to the descent of a process of parietal peritoneum containing omentum or gut into and distending the femoral canal in the thigh. Would such a swelling, before the process of peritoneum had broken through the walls of the canal into the subcutaneous tissues of the groin, pass for a femoral hernia? Fortunately this problem does not arise. A study of the posterior aspect of the anterior abdominal wall, removed with the attached anterior half of the bony pelvis, reveals many details of the design and attachments of the deep inguinal ring, the femoral trough and canals and the femoral sheath.", "contents": "Inguinal anatomy. The flat muscles of the anterior abdominal wall pass down well in front of the pectineal ligament of Astley Cooper on the summit of the superior pubic rumus, thus providing space for the spermatic cord and the great vessels of the lower limb to leave the abdomen. The abdominal wall layers turn back lower down to close the gap on the medial side of the external iliac vein. The external oblique aponeurosis turns back to form a strong inguinal ligament, and the attached fascia lata turns back as the lacunar ligament to meet and fuse with the pectineus muscle fascia 1-1.5 cm below Cooper's ligament. This leaves a deep trough, between Cooper's ligament behind and the posterior inguinal wall in front, which houses the transversely placed femoral canal. The transversalis fascia of the floor of the femoral canal turns down to form the medial wall of the venous compartment of the femoral sheath, and has the support of the curved edge of the lacunar ligament which effectively bars the femoral canal from entering the thigh. It is usually taught, however, that the lower end of the femoral canal passes unprotected into the thigh as the medial or third compartment of the femoral sheath, to lie between the femoral vein in its sheath and the curved edge of the lacunar ligament. If this were the case there would be no hindrance to the descent of a process of parietal peritoneum containing omentum or gut into and distending the femoral canal in the thigh. Would such a swelling, before the process of peritoneum had broken through the walls of the canal into the subcutaneous tissues of the groin, pass for a femoral hernia? Fortunately this problem does not arise. A study of the posterior aspect of the anterior abdominal wall, removed with the attached anterior half of the bony pelvis, reveals many details of the design and attachments of the deep inguinal ring, the femoral trough and canals and the femoral sheath."}
{"id": "PMID:468710", "title": "Radiographic profile of the first cervical vertebra.", "content": "Lateral cephalographs from 220 normal adolescent white orthodontic patients, and of individuals with craniofacial dysostosis and oculodento-osseous dysplasia were studied. Variations were observed in the morphology of the posterior margins of the superior articular processes, vertebral artery canals being present in 8%. Posterior arch dehiscence was found in eight individuals, including seven cases in the midline. Three patients had accessory ossicles above the posterior arch of the atlas, and two showed evidence of fusion of the second and third cervical vertebrae. The profile of the atlas was well outside the normal range in both syndromes studied.", "contents": "Radiographic profile of the first cervical vertebra. Lateral cephalographs from 220 normal adolescent white orthodontic patients, and of individuals with craniofacial dysostosis and oculodento-osseous dysplasia were studied. Variations were observed in the morphology of the posterior margins of the superior articular processes, vertebral artery canals being present in 8%. Posterior arch dehiscence was found in eight individuals, including seven cases in the midline. Three patients had accessory ossicles above the posterior arch of the atlas, and two showed evidence of fusion of the second and third cervical vertebrae. The profile of the atlas was well outside the normal range in both syndromes studied."}
{"id": "PMID:468711", "title": "The seminiferous tubules of the testis before, around and after birth.", "content": "A study has been carried out on the width of the seminiferous tubules, and the proportion of pre-germinal cells, in 174 stillborn children between 24 and 40 weeks of gestation, and in 146 children dying between the ages of birth and six years. There was a general diminution in the diameter of the seminiferous tubule throughout the last trimester and immediately before birth, followed by a slow and steady increase in the diameter of the tubule throughout childhood. The germinal cells within the seminiferous tubules appear to be under some slightly increased stimulatory influence during the last few weeks prior to delivery, and under considerable stimulation in the first 4 or 5 weeks after birth. Following this these cells appear to diminish in size and become hidden among the Sertoli cells, to be re-stimulated around the time of puberty.", "contents": "The seminiferous tubules of the testis before, around and after birth. A study has been carried out on the width of the seminiferous tubules, and the proportion of pre-germinal cells, in 174 stillborn children between 24 and 40 weeks of gestation, and in 146 children dying between the ages of birth and six years. There was a general diminution in the diameter of the seminiferous tubule throughout the last trimester and immediately before birth, followed by a slow and steady increase in the diameter of the tubule throughout childhood. The germinal cells within the seminiferous tubules appear to be under some slightly increased stimulatory influence during the last few weeks prior to delivery, and under considerable stimulation in the first 4 or 5 weeks after birth. Following this these cells appear to diminish in size and become hidden among the Sertoli cells, to be re-stimulated around the time of puberty."}
{"id": "PMID:468712", "title": "Observations on the primordial oocyte of the bandicoot Isoodon macrourus (Peramelidae, Marsupialia).", "content": "An ultrastructural study of bandicoot primordial follicles and oocytes was undertaken, as information on this subject is lacking in marsupials. Conspiculous features of the ooplasm are a paranuclear complex (PNC), a vesicle-microtubule complex (VMC) and an aggregate of tubular cisternae (ATC). The PNCappears as one or, more rarely, several homogeneous eosinophil bodies at the light microscope level. Ultrastructurally it is particulate, consisting of five distinct types of bodies, most of which are composed of concentric fibrillar whorls, but others appear homogeneous, granular or crystalline. Embedded among the particles is a group of Golgi-like vesicles. The bandicoot PNC-unlike similar structures found in the ooplasm of a variety of vertebrates, and known variously as \"Balbiani body\", \"yolk nucleus\", etc.-totally lacks nitochondria. The VMC consists of vesicle-like organelles which may be drawn out into tubular extensions, while the bounding membrane may be decorated with granules. Bundles of microtubules ramify between the vesicles, from which they appear to originate. The vesicles contain a matrix similar to the ooplasm. The ATC contains a homogeneous substance more electron-dense than the surrounding ooplasm. 'Dense bodies' occur in the cytoplasm of both the follicle cells and the oocytes. These are elongate membrane-bound organelles, circular in cross section. An electron-dense core is separated from the membrane by a narrow, less dense zone. The genesis and morphogenetic significance of these various organelles is unknown.", "contents": "Observations on the primordial oocyte of the bandicoot Isoodon macrourus (Peramelidae, Marsupialia). An ultrastructural study of bandicoot primordial follicles and oocytes was undertaken, as information on this subject is lacking in marsupials. Conspiculous features of the ooplasm are a paranuclear complex (PNC), a vesicle-microtubule complex (VMC) and an aggregate of tubular cisternae (ATC). The PNCappears as one or, more rarely, several homogeneous eosinophil bodies at the light microscope level. Ultrastructurally it is particulate, consisting of five distinct types of bodies, most of which are composed of concentric fibrillar whorls, but others appear homogeneous, granular or crystalline. Embedded among the particles is a group of Golgi-like vesicles. The bandicoot PNC-unlike similar structures found in the ooplasm of a variety of vertebrates, and known variously as \"Balbiani body\", \"yolk nucleus\", etc.-totally lacks nitochondria. The VMC consists of vesicle-like organelles which may be drawn out into tubular extensions, while the bounding membrane may be decorated with granules. Bundles of microtubules ramify between the vesicles, from which they appear to originate. The vesicles contain a matrix similar to the ooplasm. The ATC contains a homogeneous substance more electron-dense than the surrounding ooplasm. 'Dense bodies' occur in the cytoplasm of both the follicle cells and the oocytes. These are elongate membrane-bound organelles, circular in cross section. An electron-dense core is separated from the membrane by a narrow, less dense zone. The genesis and morphogenetic significance of these various organelles is unknown."}
{"id": "PMID:468713", "title": "Herbimycin, a new antibiotic produced by a strain of Streptomyces.", "content": "Herbimycin, a new antibiotic, was isolated from the fermentation broth of Streptomyces hygroscopicus strain No. AM-3672, a soil isolate. The molecular formula of herbimycin was determined to be C30H42N2O9. Herbimycin was found to have potent herbicidal activity against most mono- and di-cotyledonous plants, especially against Cyperus microiria STEUD. However, Oryza sativa showed strong resistance to herbimycin.", "contents": "Herbimycin, a new antibiotic produced by a strain of Streptomyces. Herbimycin, a new antibiotic, was isolated from the fermentation broth of Streptomyces hygroscopicus strain No. AM-3672, a soil isolate. The molecular formula of herbimycin was determined to be C30H42N2O9. Herbimycin was found to have potent herbicidal activity against most mono- and di-cotyledonous plants, especially against Cyperus microiria STEUD. However, Oryza sativa showed strong resistance to herbimycin."}
{"id": "PMID:468714", "title": "PS-5, a new beta-lactam antibiotic. I. Taxonomy of the producing organism, isolation and physico-chemical properties.", "content": "Antibiotic PS-5 is a new beta-lactam antibiotic isolated from fermentation broths of Streptomyces sp. strain A271. The strain was considered to be a new subspecies of Streptomyces cremeus and the name, Streptomyces cremeus subsp. auratilis, was proposed. Fermentative production, isolation and physico-chemical properties of PS-5 are described.", "contents": "PS-5, a new beta-lactam antibiotic. I. Taxonomy of the producing organism, isolation and physico-chemical properties. Antibiotic PS-5 is a new beta-lactam antibiotic isolated from fermentation broths of Streptomyces sp. strain A271. The strain was considered to be a new subspecies of Streptomyces cremeus and the name, Streptomyces cremeus subsp. auratilis, was proposed. Fermentative production, isolation and physico-chemical properties of PS-5 are described."}
{"id": "PMID:468715", "title": "Olivanic acids, a family of beta-lactam antibiotics with beta-lactamase inhibitory properties produced by Streptomyces species. I. Detection, properties and fermentation studies.", "content": "The screening of soil actinomyces for beta-lactamase inhibitors is described. Using a plate test a number of strains of Streptomyces were found to produce beta-lactamase inhibitory activity designated olivanic acid complex. Factors affecting the production of this complex by Streptomyces olivaceus ATCC 21379 are reported. The complex showed antibacterial activity and also inhibited a number of different types of beta-lactamase in a progressive manner. Certain ampicillin-resistant bacteria are rendered sensitive to ampicillin in the presence of olivanic acid complex at a concentration which alone did not inhibit growth.", "contents": "Olivanic acids, a family of beta-lactam antibiotics with beta-lactamase inhibitory properties produced by Streptomyces species. I. Detection, properties and fermentation studies. The screening of soil actinomyces for beta-lactamase inhibitors is described. Using a plate test a number of strains of Streptomyces were found to produce beta-lactamase inhibitory activity designated olivanic acid complex. Factors affecting the production of this complex by Streptomyces olivaceus ATCC 21379 are reported. The complex showed antibacterial activity and also inhibited a number of different types of beta-lactamase in a progressive manner. Certain ampicillin-resistant bacteria are rendered sensitive to ampicillin in the presence of olivanic acid complex at a concentration which alone did not inhibit growth."}
{"id": "PMID:468716", "title": "Olivanic acids, a family of beta-lactam antibiotics with beta-lactamase inhibitory properties produced by Streptomyces species. II. Isolation and characterisation of the olivanic acids MM 4550, MM 13902 and MM 17880 from Streptomyces olivaceus.", "content": "The olivanic acids MM 4550, MM 13902 and MM 17880 are members of a new family of beta-lactam antibiotics. An isolation and purification process utilising ion-par extraction and ion-exchange chromatography is described and the metabolites are characterised by physico-chemical and biological properties.", "contents": "Olivanic acids, a family of beta-lactam antibiotics with beta-lactamase inhibitory properties produced by Streptomyces species. II. Isolation and characterisation of the olivanic acids MM 4550, MM 13902 and MM 17880 from Streptomyces olivaceus. The olivanic acids MM 4550, MM 13902 and MM 17880 are members of a new family of beta-lactam antibiotics. An isolation and purification process utilising ion-par extraction and ion-exchange chromatography is described and the metabolites are characterised by physico-chemical and biological properties."}
{"id": "PMID:468717", "title": "The structures of tridecaptins B and C (studies on antibiotics from the genus Bacillus. XXV).", "content": "On examining the structures of the antibiotics tridecaptins B and C, the constituent amino acids were separated and their chiralities were determined. The constituent fatty acids were identified by gas chromatography and mass spectrometry. Cleavage with N-bromosuccinimide and sequential analysis by EDMAN degradation demonstrates the sequence of the C-terminal side of tridecaptins B and C. Deacylation with polymyxin acylase of tridecaptin B and successive EDMAN degradation revealed the sequence of the N-terminal side of tridecaptin B. Finally partial acid hydrolysis on tridecaptin C clarified the sequence of the N-terminal side of tridecaptin C.", "contents": "The structures of tridecaptins B and C (studies on antibiotics from the genus Bacillus. XXV). On examining the structures of the antibiotics tridecaptins B and C, the constituent amino acids were separated and their chiralities were determined. The constituent fatty acids were identified by gas chromatography and mass spectrometry. Cleavage with N-bromosuccinimide and sequential analysis by EDMAN degradation demonstrates the sequence of the C-terminal side of tridecaptins B and C. Deacylation with polymyxin acylase of tridecaptin B and successive EDMAN degradation revealed the sequence of the N-terminal side of tridecaptin B. Finally partial acid hydrolysis on tridecaptin C clarified the sequence of the N-terminal side of tridecaptin C."}
{"id": "PMID:468718", "title": "Resolution of peptide antibiotics, cerexins and tridecaptins, by high performance liquid chromatography (studies on antibiotics from the genus Bacillus. XXVI).", "content": "By high performance liquid chromatography, cerexin B was separated into four components (B1, B2, B3 and B4), cerexin D into four components (D1, D2, D3 and D4), tridecaptin A into components (A alpha and A beta), tridecaptin B into four components (B alpha, B beta, B gamma and B delta) and tridecaptin C into three components (C alpha 1, C alpha 2 and C beta 1). All components were preparatively isolated, and their fatty acid and amino acid compositions determined for structural elucidation.", "contents": "Resolution of peptide antibiotics, cerexins and tridecaptins, by high performance liquid chromatography (studies on antibiotics from the genus Bacillus. XXVI). By high performance liquid chromatography, cerexin B was separated into four components (B1, B2, B3 and B4), cerexin D into four components (D1, D2, D3 and D4), tridecaptin A into components (A alpha and A beta), tridecaptin B into four components (B alpha, B beta, B gamma and B delta) and tridecaptin C into three components (C alpha 1, C alpha 2 and C beta 1). All components were preparatively isolated, and their fatty acid and amino acid compositions determined for structural elucidation."}
{"id": "PMID:468720", "title": "Studies on auromomycin.", "content": "A new antitumor antibiotic, named auromomycin, was isolated from the culture broth of Streptomyces macromomyceticus, a macromomycin-producing strain. The antibiotic was recovered from the culture filtrate by salting out with ammonium sulfate and further purified by successive application of ion-exchange chromatography on Amberlite IRA-93 (Cl form) and DEAE-Sephadex (OH form), Gel filtration on Sephadex G-50 and hydrophobic chromatography on Octyl-Sepharose CL-4B. The antibiotic is an acidic polypeptide with a molecular weitht of 12,500 and an isoelectric point of pH 5.4 and consists of 16 different amino acids. It has characteristic absorption maxima at 273 nm and 357 nm in the ultraviolet spectrum and two minima at 280 nm and 350 nm in the optical rotatory dispersion spectrum. Auromomycin exhibits antibacterial activity not only against Gram-positive bacteria, but also Gram-negative bacteria. Antitumor activities of auromomycin were revealed against EHRLICH ascites carcinoma, ascites sarcoma 180, L1210 leukemia and LEWIS lung carcinoma. Auromomycin was found to be converted into macromomycin by adsorption chromatography on Amberlite XAD.", "contents": "Studies on auromomycin. A new antitumor antibiotic, named auromomycin, was isolated from the culture broth of Streptomyces macromomyceticus, a macromomycin-producing strain. The antibiotic was recovered from the culture filtrate by salting out with ammonium sulfate and further purified by successive application of ion-exchange chromatography on Amberlite IRA-93 (Cl form) and DEAE-Sephadex (OH form), Gel filtration on Sephadex G-50 and hydrophobic chromatography on Octyl-Sepharose CL-4B. The antibiotic is an acidic polypeptide with a molecular weitht of 12,500 and an isoelectric point of pH 5.4 and consists of 16 different amino acids. It has characteristic absorption maxima at 273 nm and 357 nm in the ultraviolet spectrum and two minima at 280 nm and 350 nm in the optical rotatory dispersion spectrum. Auromomycin exhibits antibacterial activity not only against Gram-positive bacteria, but also Gram-negative bacteria. Antitumor activities of auromomycin were revealed against EHRLICH ascites carcinoma, ascites sarcoma 180, L1210 leukemia and LEWIS lung carcinoma. Auromomycin was found to be converted into macromomycin by adsorption chromatography on Amberlite XAD."}
{"id": "PMID:468721", "title": "Biological activity of macromomycin.", "content": "Macromomycin (MCR) is a polypeptide antimuor antibiotic isolated from the culture broth of Streptomyces macromomyceticus. Antitumor activities of MCR were examined against three different tumor system, i.e., EHRLICH ascites carcinoma, L1210 leukemia and LEWIS lung carcinoma. Daily intraperitoneal treatment with MCR for 5 days showed a strong inhibition against EHRLICH ascites carcinoma. Both single and repeated intraperitoneal injections of MCR were effective over a wide dose range against intraperitoneally inoculated L1210 leukemia and MCR intravenously administered was also active against intravenously inoculated L1210 leukemia. Daily local subcutaneous injections of MCR produced the prolongation of life span of mice to which LEWIS lung carcinoma was subcutaneously inoculated with some cured mice, but daily intraperitoneal injections of MCR showed no activity. Single intravenous administration of MCR inhibited early LEWIS lung carcinoma, but not advanced LEWIS lung carcinoma. The combination of MCR with aracytidine, or cyclophosphamide showed a synergistic activity against L1210 leukemia. MCR was not inactivated by treatment with serum, although neocarzinostatin was markedly inactivated by the same treatment.", "contents": "Biological activity of macromomycin. Macromomycin (MCR) is a polypeptide antimuor antibiotic isolated from the culture broth of Streptomyces macromomyceticus. Antitumor activities of MCR were examined against three different tumor system, i.e., EHRLICH ascites carcinoma, L1210 leukemia and LEWIS lung carcinoma. Daily intraperitoneal treatment with MCR for 5 days showed a strong inhibition against EHRLICH ascites carcinoma. Both single and repeated intraperitoneal injections of MCR were effective over a wide dose range against intraperitoneally inoculated L1210 leukemia and MCR intravenously administered was also active against intravenously inoculated L1210 leukemia. Daily local subcutaneous injections of MCR produced the prolongation of life span of mice to which LEWIS lung carcinoma was subcutaneously inoculated with some cured mice, but daily intraperitoneal injections of MCR showed no activity. Single intravenous administration of MCR inhibited early LEWIS lung carcinoma, but not advanced LEWIS lung carcinoma. The combination of MCR with aracytidine, or cyclophosphamide showed a synergistic activity against L1210 leukemia. MCR was not inactivated by treatment with serum, although neocarzinostatin was markedly inactivated by the same treatment."}
{"id": "PMID:468722", "title": "Antitumor activity of prumycin.", "content": "The antifungal antibiotic, prumycin, was studied for antitumor activity against several tumor systems. It was found to possess potential antitumor activity against a well-established mouse mammary adenocarcinoma in C3H/He mice. It was also active in prolongation of the lifespan of mice bearing P-388 lymphocytic leukemia. Moreover, prumycin did not depress the white blood cell counts in the mouse peripheral blood. However, severe alopecia was observed in mice treated with this agent at dosage level near the LD50.", "contents": "Antitumor activity of prumycin. The antifungal antibiotic, prumycin, was studied for antitumor activity against several tumor systems. It was found to possess potential antitumor activity against a well-established mouse mammary adenocarcinoma in C3H/He mice. It was also active in prolongation of the lifespan of mice bearing P-388 lymphocytic leukemia. Moreover, prumycin did not depress the white blood cell counts in the mouse peripheral blood. However, severe alopecia was observed in mice treated with this agent at dosage level near the LD50."}
{"id": "PMID:468723", "title": "13C-NMR studies on 1-N-[(S)-4-amino-2-hydroxybutyl]kanamycin A (butikacin) and related aminoglycosides.", "content": "The 13C-NMR spectra of 1-N-[(S)-4-amino-2-hydroxybutyl]kanamycin A (butikacin) (Fig. 1, 1b) and some of its related compounds have been recorded and are tabulated, assigned and discussed. The chemical shifts of many of the carbon nuclei are shown to be reasonably invariant amongst this series of compounds. A procedure is described for the determination of the (R,S)-epimer ratio of kanamycin A derivatives which have N-substituted groups containing a chiral centre.", "contents": "13C-NMR studies on 1-N-[(S)-4-amino-2-hydroxybutyl]kanamycin A (butikacin) and related aminoglycosides. The 13C-NMR spectra of 1-N-[(S)-4-amino-2-hydroxybutyl]kanamycin A (butikacin) (Fig. 1, 1b) and some of its related compounds have been recorded and are tabulated, assigned and discussed. The chemical shifts of many of the carbon nuclei are shown to be reasonably invariant amongst this series of compounds. A procedure is described for the determination of the (R,S)-epimer ratio of kanamycin A derivatives which have N-substituted groups containing a chiral centre."}
{"id": "PMID:468724", "title": "Chemical modification of fortimicins: preparation of 4-N-substituted fortimicin B.", "content": "Among the new aminoglycoside antibiotic family for fortimicins, components A, C and D have higher activity compared to their 4-N-deacylated components B and KE. Synthesis and antibacterial activities of 4-N-acyl- and 4-N-alkyl-fortimicin B derivatives are described. 4-N-Acylfortimicin B's, which are relatively unstable in alkaline conditions, were converted to stable 4-N-alkyl derivatives with diborane. The activity is greatly affected by the 4-N-substituents, and the presence of hydrophilic group(s) is necessary to confer activity on the derivatives. 4-N-(2-Aminoethyl)-, 4-N-(4-amino-2-hydroxybutyl)- and 4-N-(2-hydroxy-4-methylaminobutyl)-fortimicin B are the most potent compounds among them.", "contents": "Chemical modification of fortimicins: preparation of 4-N-substituted fortimicin B. Among the new aminoglycoside antibiotic family for fortimicins, components A, C and D have higher activity compared to their 4-N-deacylated components B and KE. Synthesis and antibacterial activities of 4-N-acyl- and 4-N-alkyl-fortimicin B derivatives are described. 4-N-Acylfortimicin B's, which are relatively unstable in alkaline conditions, were converted to stable 4-N-alkyl derivatives with diborane. The activity is greatly affected by the 4-N-substituents, and the presence of hydrophilic group(s) is necessary to confer activity on the derivatives. 4-N-(2-Aminoethyl)-, 4-N-(4-amino-2-hydroxybutyl)- and 4-N-(2-hydroxy-4-methylaminobutyl)-fortimicin B are the most potent compounds among them."}
{"id": "PMID:468725", "title": "Congeners of etamycin produced by Streptomyces griseoviridus.", "content": "Streptomyces griseoviridus produces in addition to etamycin several related compounds which can be separated by partition chromatography. One of these has been characterized by amino acid analysis and mass spectrometry and shown to have the same structure as etamycin except for replacement of the hydroxyproline residue by proline. Evidence was obtained for additional congeners similarly related to etamycin by amino acid exchange. The relative proportions of such congeners produced by S. griseoviridus depends upon the medium in which the culture is grown. Certain amino acids support good yields of the metabolites and the culture appears to be steered towards the synthesis of congeners containing such amino acids.", "contents": "Congeners of etamycin produced by Streptomyces griseoviridus. Streptomyces griseoviridus produces in addition to etamycin several related compounds which can be separated by partition chromatography. One of these has been characterized by amino acid analysis and mass spectrometry and shown to have the same structure as etamycin except for replacement of the hydroxyproline residue by proline. Evidence was obtained for additional congeners similarly related to etamycin by amino acid exchange. The relative proportions of such congeners produced by S. griseoviridus depends upon the medium in which the culture is grown. Certain amino acids support good yields of the metabolites and the culture appears to be steered towards the synthesis of congeners containing such amino acids."}
{"id": "PMID:468726", "title": "Effect of primycin on monovalent cation transport of erythrocyte membrane and lipid bilayer.", "content": "The effects of primycin were investigated on the alkali-cation transport of human erythrocytes and on the electric conduction of bimolecular lipid membranes. In the concentration range of 3.10(-6) approximately 10(-5) M primycin increased the permeability of erythrocytes to alkali-cations according to the sequences Cs+ greater than Rb+ approximately K+ greater than Na+, while the conductance of the negatively charged phosphatidylserine bimolecular lipid membrane increased by 2 approximately 3 orders of magnitude. The resistance-lowering effect of primycin strongly depended on the cationic species applied and a selectivity order Na+ greater than K+ greater than Rb+ greater than Cs+ was found. A possible mechanism of the primycin-membrane interaction is suggested on the basis of experimental data.", "contents": "Effect of primycin on monovalent cation transport of erythrocyte membrane and lipid bilayer. The effects of primycin were investigated on the alkali-cation transport of human erythrocytes and on the electric conduction of bimolecular lipid membranes. In the concentration range of 3.10(-6) approximately 10(-5) M primycin increased the permeability of erythrocytes to alkali-cations according to the sequences Cs+ greater than Rb+ approximately K+ greater than Na+, while the conductance of the negatively charged phosphatidylserine bimolecular lipid membrane increased by 2 approximately 3 orders of magnitude. The resistance-lowering effect of primycin strongly depended on the cationic species applied and a selectivity order Na+ greater than K+ greater than Rb+ greater than Cs+ was found. A possible mechanism of the primycin-membrane interaction is suggested on the basis of experimental data."}
{"id": "PMID:468729", "title": "Holomycin and an antibiotic (MM 19290) related to tunicamycin, metabolites of Streptomyces clavuligerus.", "content": "Streptomyces clavuligerus produces penicillin N, several cephalosporins and the beta-lactamase inhibitor clavulanic acid. The detection, isolation and properties of further metabolites of this culture, MM 21801 and MM 19290, are described. MM 21801 was identified as the antibiotic holomycin. MM 19290 was shown to be related to tunicamycin, an antibiotic complex obtained from cultures of Streptomyces lysosuperificus.", "contents": "Holomycin and an antibiotic (MM 19290) related to tunicamycin, metabolites of Streptomyces clavuligerus. Streptomyces clavuligerus produces penicillin N, several cephalosporins and the beta-lactamase inhibitor clavulanic acid. The detection, isolation and properties of further metabolites of this culture, MM 21801 and MM 19290, are described. MM 21801 was identified as the antibiotic holomycin. MM 19290 was shown to be related to tunicamycin, an antibiotic complex obtained from cultures of Streptomyces lysosuperificus."}
{"id": "PMID:468730", "title": "The structure of nocamycin, a new antitumor antibiotic.", "content": "The structure of nocamycin, a new antitumor antibiotic, has been elucidated with the aid of mass- and PMR-spectroscopic investigation of the antibiotic and its various chemical transformation products. Nocamycin is structurally related to tirandamycins.", "contents": "The structure of nocamycin, a new antitumor antibiotic. The structure of nocamycin, a new antitumor antibiotic, has been elucidated with the aid of mass- and PMR-spectroscopic investigation of the antibiotic and its various chemical transformation products. Nocamycin is structurally related to tirandamycins."}
{"id": "PMID:468731", "title": "3-Hydroxyterphenyllin, a new metabolite of Aspergillus candidus. Structure elucidation by H and C nuclear magnetic resonance spectroscopy.", "content": "A new compound isolated from cultures of Aspergillus candidus Link is shown to be 3-hydroxyterphenyllin. The structure was assigned by comparing the 1H and 13C nmr spectra of the metabolite and its acetate derivative with those of terphenyllin and terphenyllin triacetate.", "contents": "3-Hydroxyterphenyllin, a new metabolite of Aspergillus candidus. Structure elucidation by H and C nuclear magnetic resonance spectroscopy. A new compound isolated from cultures of Aspergillus candidus Link is shown to be 3-hydroxyterphenyllin. The structure was assigned by comparing the 1H and 13C nmr spectra of the metabolite and its acetate derivative with those of terphenyllin and terphenyllin triacetate."}
{"id": "PMID:468732", "title": "The structure of a novel sugar component of polyene macrolide antibiotics: 2,6-dideoxy-L-ribohexopyranose.", "content": "A novel carbohydrate has been isolated after acidic hydrolysis of nystatin A3, candidinin and polyfungin B and its structure established as 2,6-dideoxy-L-ribohexopyranose.", "contents": "The structure of a novel sugar component of polyene macrolide antibiotics: 2,6-dideoxy-L-ribohexopyranose. A novel carbohydrate has been isolated after acidic hydrolysis of nystatin A3, candidinin and polyfungin B and its structure established as 2,6-dideoxy-L-ribohexopyranose."}
{"id": "PMID:468733", "title": "The identification of depsipeptides by chemical ionisation mass spectroscopy.", "content": "The chemical ionisation mass spectra of seven naturally occurring depsipeptides and some of their permethylated derivatives have been measured. The primary ionisation process involves an ester group and not an amide or other functionality. It probably occurs randomly when the molecule contains more than one ester link. Unlike electron impact mass spectra, those obtained under chemical ionisation conditions gave sequence information for all of the depsipeptides examined or their permethylated derivatives. A mechanism for the primary fragmentation is proposed.", "contents": "The identification of depsipeptides by chemical ionisation mass spectroscopy. The chemical ionisation mass spectra of seven naturally occurring depsipeptides and some of their permethylated derivatives have been measured. The primary ionisation process involves an ester group and not an amide or other functionality. It probably occurs randomly when the molecule contains more than one ester link. Unlike electron impact mass spectra, those obtained under chemical ionisation conditions gave sequence information for all of the depsipeptides examined or their permethylated derivatives. A mechanism for the primary fragmentation is proposed."}
{"id": "PMID:468734", "title": "Controlled biosynthesis of neoviridogriseins, new homologues of viridogrisein II. Production, biological properties and structure of neoviridogrisein II.", "content": "Our isolate of Streptomyces griseoviridus, which produced three minor factors in addition to viridogrisein and griseoviridus, which produced three minor factors in addition to viridogrisein and griseoviridin, was more sensitive to the addition of L-proline than the type culture of Streptomyces griseoviridus (NRRL2427). Yield improvement of these minor factors was attempted by the so-called controlled biosynthesis with L-proline. Addition of L-proline increased the production of neoviridogrisein II in which allo-hydroxy-D-proline in viridogrisein was replaced by D-proline.", "contents": "Controlled biosynthesis of neoviridogriseins, new homologues of viridogrisein II. Production, biological properties and structure of neoviridogrisein II. Our isolate of Streptomyces griseoviridus, which produced three minor factors in addition to viridogrisein and griseoviridus, which produced three minor factors in addition to viridogrisein and griseoviridin, was more sensitive to the addition of L-proline than the type culture of Streptomyces griseoviridus (NRRL2427). Yield improvement of these minor factors was attempted by the so-called controlled biosynthesis with L-proline. Addition of L-proline increased the production of neoviridogrisein II in which allo-hydroxy-D-proline in viridogrisein was replaced by D-proline."}
{"id": "PMID:468735", "title": "Bioconversion and biosynthesis of 16-membered macrolide antibiotics. XIII. Regulation of spiramycin I 3-hydroxyl acylase formation by glucose, butyrate, and cerulenin.", "content": "The effects of glucose, butyrate, and cerulenin on the formation of spiramycin I 3-hydroxyl acylase were investigated by using the cell-free extract prepared from the spiramycin-producing strain of Streptomyces ambofaciens KA-1028. Glucose induced the formation of the acylase, and this induction was remarkably repressed by butyrate. Cerulenin, on the other hand, not only cancelled the repression by butyrate but also stimulated the formation of the acylase. The unsuccessful trapping of spiramycin I as an intermediate during the bioconversion from neospiramycin I to spiramycin III in the presence of cerulenin was due to the rapid acylation of spiramycin I caused by the acylase induced by cerulenin.", "contents": "Bioconversion and biosynthesis of 16-membered macrolide antibiotics. XIII. Regulation of spiramycin I 3-hydroxyl acylase formation by glucose, butyrate, and cerulenin. The effects of glucose, butyrate, and cerulenin on the formation of spiramycin I 3-hydroxyl acylase were investigated by using the cell-free extract prepared from the spiramycin-producing strain of Streptomyces ambofaciens KA-1028. Glucose induced the formation of the acylase, and this induction was remarkably repressed by butyrate. Cerulenin, on the other hand, not only cancelled the repression by butyrate but also stimulated the formation of the acylase. The unsuccessful trapping of spiramycin I as an intermediate during the bioconversion from neospiramycin I to spiramycin III in the presence of cerulenin was due to the rapid acylation of spiramycin I caused by the acylase induced by cerulenin."}
{"id": "PMID:468736", "title": "Genetics and biochemical studies of chloramphenicol-nonproducing mutants of Streptomyces venezuelae carrying plasmid.", "content": "Chloramphenicol-nonproducing and plasmid-less mutants obtained previously by treatment with acriflavine still produced a small amount of chloramphenicol in a medium. To study the role of plasmid in chloramphenicol production, 70 chloramphenicol-nonproducing mutants were isolated by acriflavine treatment, high-temperature incubation, UV-irradiation or nitrosoguanidine treatment, starting from a producer (SVM2). Most of them did not produce any amount of chloramphenicol. One mutant, SVM2-2A7 was found to produce 1-deoxychloramphenicol instead of chloramphenicol. The mutations (cpp) affecting chloramphenicol production were analyzed by crosses with a producing strain carrying the complementing auxotrophic markers. Except for the plasmid-less strains, all Cpp mutations including the 1-deoxychloramphenicol-producing mutation were mapped between met and ilv on the chromosome. Additional crosses indicated that these chromosomal cpp mutants still carried the plasmids which had a role in increasing chloramphenicol production. Therefore, it can be concluded that the structural genes for all or most steps of chloramphenicol biosynthesis including the 3-hydroxylation of p-aminophenylalanine are located between met and ilv on the chromosome of S. venezuelae and that the plasmid plays an important role in increasing the chloramphenicol production. The activity of arylamine synthetase involved in the initial step of the chloramphenicol biosynthesis was unrelated to the presence or absence of plasmid. Moreover, the presence of plasmids was not required for host resistance to chloramphenicol.", "contents": "Genetics and biochemical studies of chloramphenicol-nonproducing mutants of Streptomyces venezuelae carrying plasmid. Chloramphenicol-nonproducing and plasmid-less mutants obtained previously by treatment with acriflavine still produced a small amount of chloramphenicol in a medium. To study the role of plasmid in chloramphenicol production, 70 chloramphenicol-nonproducing mutants were isolated by acriflavine treatment, high-temperature incubation, UV-irradiation or nitrosoguanidine treatment, starting from a producer (SVM2). Most of them did not produce any amount of chloramphenicol. One mutant, SVM2-2A7 was found to produce 1-deoxychloramphenicol instead of chloramphenicol. The mutations (cpp) affecting chloramphenicol production were analyzed by crosses with a producing strain carrying the complementing auxotrophic markers. Except for the plasmid-less strains, all Cpp mutations including the 1-deoxychloramphenicol-producing mutation were mapped between met and ilv on the chromosome. Additional crosses indicated that these chromosomal cpp mutants still carried the plasmids which had a role in increasing chloramphenicol production. Therefore, it can be concluded that the structural genes for all or most steps of chloramphenicol biosynthesis including the 3-hydroxylation of p-aminophenylalanine are located between met and ilv on the chromosome of S. venezuelae and that the plasmid plays an important role in increasing the chloramphenicol production. The activity of arylamine synthetase involved in the initial step of the chloramphenicol biosynthesis was unrelated to the presence or absence of plasmid. Moreover, the presence of plasmids was not required for host resistance to chloramphenicol."}
{"id": "PMID:468737", "title": "CI-867, a new broad-spectrum semisynthetic penicillin.", "content": "The synthesis and antimicrobial activity of a new semisynthetic penicillin are described. Both in vitro and in vivo, the compound shows promising antibacterial activity when compared with piperacillin and ticarcillin. High activity is shown against Pseudomonas and other Gram-negative bacteria.", "contents": "CI-867, a new broad-spectrum semisynthetic penicillin. The synthesis and antimicrobial activity of a new semisynthetic penicillin are described. Both in vitro and in vivo, the compound shows promising antibacterial activity when compared with piperacillin and ticarcillin. High activity is shown against Pseudomonas and other Gram-negative bacteria."}
{"id": "PMID:468738", "title": "Antibacterial activity of heneicomycin.", "content": "Heneicomycin is structurally similar to efrotomycin, mocimycin (kirromycin) and X-5108 (goldinomycin). Comparisons were limited because of the small supplies available. All antibiotics show the same in vitro antibacterial spectrum although some test cultures were less sensitive to efrotomycin. Heneicomycin compared favorably with efrotomycin when given subcutaneously or per os against infections with Moraxella bovis and Streptococcus pyogenes. The raid elimination of heneicomycin observed following oral administration may account for its poor activity against a Bordetella bronchiseptica infection where efrotomycin is effective. It appears more like X-5108 than efrotomycin biologically. The disaccharide on efrotomycin may account for the difference observed.", "contents": "Antibacterial activity of heneicomycin. Heneicomycin is structurally similar to efrotomycin, mocimycin (kirromycin) and X-5108 (goldinomycin). Comparisons were limited because of the small supplies available. All antibiotics show the same in vitro antibacterial spectrum although some test cultures were less sensitive to efrotomycin. Heneicomycin compared favorably with efrotomycin when given subcutaneously or per os against infections with Moraxella bovis and Streptococcus pyogenes. The raid elimination of heneicomycin observed following oral administration may account for its poor activity against a Bordetella bronchiseptica infection where efrotomycin is effective. It appears more like X-5108 than efrotomycin biologically. The disaccharide on efrotomycin may account for the difference observed."}
{"id": "PMID:468739", "title": "Studies on inhibition of adenosine deaminase by isocoformycin in vitro and in vivo.", "content": "Isocoformycin is a structural isomer of coformycin which has been demonstrated to be a potent inhibitor of adenosine deaminase. Isocoformycin showed a weaker inhibition of this enzyme than coformycin; the binding of coformycin to enzyme was irreversible, but isocoformycin inhibition was competitive with substrate. The Ki value of isocoformycin was 4.5 approximately 10 X 10(-8) M. Following intraperitoneal injection of isocoformycin in mice, the adenosine deaminase activity of homogenates of several organs was determined and the following ED50 values (50% inhibition doses) were observed: 29 mg/kg for thymus, 13 mg/kg for spleen, 80 mg/kg for liver and 20 mg/kg for kidney. The inhibition of adenosine deaminase in rabbit blood in vitro was also tested in comparison with coformycin.", "contents": "Studies on inhibition of adenosine deaminase by isocoformycin in vitro and in vivo. Isocoformycin is a structural isomer of coformycin which has been demonstrated to be a potent inhibitor of adenosine deaminase. Isocoformycin showed a weaker inhibition of this enzyme than coformycin; the binding of coformycin to enzyme was irreversible, but isocoformycin inhibition was competitive with substrate. The Ki value of isocoformycin was 4.5 approximately 10 X 10(-8) M. Following intraperitoneal injection of isocoformycin in mice, the adenosine deaminase activity of homogenates of several organs was determined and the following ED50 values (50% inhibition doses) were observed: 29 mg/kg for thymus, 13 mg/kg for spleen, 80 mg/kg for liver and 20 mg/kg for kidney. The inhibition of adenosine deaminase in rabbit blood in vitro was also tested in comparison with coformycin."}
{"id": "PMID:468744", "title": "Nicotine fading and self-monitoring for cigarette abstinence or controlled smoking.", "content": "This study compared four treatment approaches to cigarette smoking: (1) a nicotine fading procedure, in which subjects changed their cigarette brands each week to ones containing progressively less nicotine and tar; (2) a self-monitoring procedure in which subjects plotted their daily intake of nicotine and tar; (3) a combined nicotine fading/self-monitoring procedure; and (4) a slightly modified American Cancer Society Stop Smoking Program. Thirty-eight habitual smokers were assigned to one of the treatment groups. The study had two goals: (1) to achieve a clinically significant percentage of abstinence, and (2) to reduce the nonabstainers' smoking to a \"safer\" level by having them smoke low tar and nicotine cigarettes. The 18-month followup results showed that the nicotine fading/self-monitoring group was the most successful: 40% were abstinent and all who had not quit were smoking cigarettes lower in tar and nicotine than their baseline brands. Half the nonabstainers had decreased their rate of smoking relative to baseline while the other half had increased. Furthermore, the fading/self-monitoring group achieved the largest reductions from baseline in daily nicotine and tar intake (61% and 70% respectively). The results suggest that the study's goals were achieved and that the nonaversive combined procedure could be used to treat not only habitual smokers but also smokers with severe cardiovascular and respiratory problems, because it does not have some of the inherent limitations of the successful aversive smoking cessation procedures.", "contents": "Nicotine fading and self-monitoring for cigarette abstinence or controlled smoking. This study compared four treatment approaches to cigarette smoking: (1) a nicotine fading procedure, in which subjects changed their cigarette brands each week to ones containing progressively less nicotine and tar; (2) a self-monitoring procedure in which subjects plotted their daily intake of nicotine and tar; (3) a combined nicotine fading/self-monitoring procedure; and (4) a slightly modified American Cancer Society Stop Smoking Program. Thirty-eight habitual smokers were assigned to one of the treatment groups. The study had two goals: (1) to achieve a clinically significant percentage of abstinence, and (2) to reduce the nonabstainers' smoking to a \"safer\" level by having them smoke low tar and nicotine cigarettes. The 18-month followup results showed that the nicotine fading/self-monitoring group was the most successful: 40% were abstinent and all who had not quit were smoking cigarettes lower in tar and nicotine than their baseline brands. Half the nonabstainers had decreased their rate of smoking relative to baseline while the other half had increased. Furthermore, the fading/self-monitoring group achieved the largest reductions from baseline in daily nicotine and tar intake (61% and 70% respectively). The results suggest that the study's goals were achieved and that the nonaversive combined procedure could be used to treat not only habitual smokers but also smokers with severe cardiovascular and respiratory problems, because it does not have some of the inherent limitations of the successful aversive smoking cessation procedures."}
{"id": "PMID:468745", "title": "Biofeedback and rational-emotive therapy in the management of migraine headache.", "content": "Twenty-four migraine patients were randomly assigned to one of four conditions: (a) self-monitoring of headache activity (waiting list), (b) frontalis EMG biofeedback, (c) digit temperature biofeedback, and (d) digit temperature biofeedback plus Rational-Emotive Therapy (RET). Bidirectional control over the target physiological response was assessed through a reversal design in each session. Following at least a four-week baseline, the three biofeedback groups received 8 to 10, 30-minute sessions of bidirectional biofeedback training, scheduled twice a week. Subjects in the combined digit temperature biofeedback plus RET group received three 40-minute sessions of RET as an addition to the third, fifth, and seventh biofeedback sessions. Records of daily home practice were kept throughout treatment and three-month followup. Subjects on the waiting list monitored headaches for at least five months, corresponding to \"baseline\", \"treatment\", and three-month followup. Digit temperature biofeedback alone and in conjunction with RET did not prove to be more effective than the control conditions. All the EMG subjects reduced headache activity to two-thirds or less of the baseline level by the third month of followup. Bidirectional digit temperature performance did not improve with training, was demonstrated in only 33% of the biofeedback sessions, was not maintained over time, and was unrelated to improvement in headache activity. EMG subjects reported biofeedback performance to be an easier task and met the performance criterion on 85% of the sessions. The frequency of home practice contributed over 55% of the variance in retrospective estimates of headache improvement but was not related to changes in daily records of headache activity.", "contents": "Biofeedback and rational-emotive therapy in the management of migraine headache. Twenty-four migraine patients were randomly assigned to one of four conditions: (a) self-monitoring of headache activity (waiting list), (b) frontalis EMG biofeedback, (c) digit temperature biofeedback, and (d) digit temperature biofeedback plus Rational-Emotive Therapy (RET). Bidirectional control over the target physiological response was assessed through a reversal design in each session. Following at least a four-week baseline, the three biofeedback groups received 8 to 10, 30-minute sessions of bidirectional biofeedback training, scheduled twice a week. Subjects in the combined digit temperature biofeedback plus RET group received three 40-minute sessions of RET as an addition to the third, fifth, and seventh biofeedback sessions. Records of daily home practice were kept throughout treatment and three-month followup. Subjects on the waiting list monitored headaches for at least five months, corresponding to \"baseline\", \"treatment\", and three-month followup. Digit temperature biofeedback alone and in conjunction with RET did not prove to be more effective than the control conditions. All the EMG subjects reduced headache activity to two-thirds or less of the baseline level by the third month of followup. Bidirectional digit temperature performance did not improve with training, was demonstrated in only 33% of the biofeedback sessions, was not maintained over time, and was unrelated to improvement in headache activity. EMG subjects reported biofeedback performance to be an easier task and met the performance criterion on 85% of the sessions. The frequency of home practice contributed over 55% of the variance in retrospective estimates of headache improvement but was not related to changes in daily records of headache activity."}
{"id": "PMID:468746", "title": "Comparison of established and innovative weight-reduction treatment procedures.", "content": "A central component of a commonly used weight-loss procedure developed by Stuart (1967), which emphasizes situational engineering (SE), was compared to eating behavior control (EBC), a treatment employing reinforcement of changes in eating habits. A third treatment encouraged development of individuals' own applications of a model of self-control and attempted to provide effective feedback regarding progress toward weight-loss goals. This self-initiated treatment (SI) was designed to be less directive than many behavioral treatment packages and to be more realistic about the expectations of subjects and the actual eating habits of overweight and normalweight people. Following baseline, treatment lasted six weeks and followup lasted 16 weeks. While all treatments produced weight losses, EBC surpassed SE during treatment. SI losses during treatment did not differ from SE or EBC, but SI was the only group that lost weight throughout followup, indicating the possible utility of more subtle, less directive interventions than those that have often been associated with behavioral approaches. The results of SE question the applicability to clinical procedures of the external cue hypersensitivity theory of obesity (Schachter and Rodin, 1974), to which SE is closely related.", "contents": "Comparison of established and innovative weight-reduction treatment procedures. A central component of a commonly used weight-loss procedure developed by Stuart (1967), which emphasizes situational engineering (SE), was compared to eating behavior control (EBC), a treatment employing reinforcement of changes in eating habits. A third treatment encouraged development of individuals' own applications of a model of self-control and attempted to provide effective feedback regarding progress toward weight-loss goals. This self-initiated treatment (SI) was designed to be less directive than many behavioral treatment packages and to be more realistic about the expectations of subjects and the actual eating habits of overweight and normalweight people. Following baseline, treatment lasted six weeks and followup lasted 16 weeks. While all treatments produced weight losses, EBC surpassed SE during treatment. SI losses during treatment did not differ from SE or EBC, but SI was the only group that lost weight throughout followup, indicating the possible utility of more subtle, less directive interventions than those that have often been associated with behavioral approaches. The results of SE question the applicability to clinical procedures of the external cue hypersensitivity theory of obesity (Schachter and Rodin, 1974), to which SE is closely related."}
{"id": "PMID:468747", "title": "Theoretical, practical, and social issues in behavioral treatments of obesity.", "content": "Although showing superior maintenance, behavioral treatments of obesity typically produce small weight losses at a decelerating rate. Rather than reflecting poor compliance with treatment, these findings are consistent with known compensatory metabolic changes that operate to slow weight loss and promote regain. Other problems associated with dieting include failure of caloric regulation, hyper-responsivity to food palatability, and hunger, which is greater under conditions of moderate restriction and unpredictability of access to food. The inevitability of treatment failure in many instances must be faced and efforts made to prevent further worsening of the obese patient's self-esteem. Prognosis and treatment planning may be aided by consideration of the historical difficulties of weight loss, the degree of hunger experienced on diets, which may reflect important physiological differences among individuals, and the use of food to optimize arousal level. Full involvement of the patient in setting goals and planning treatment is recommended.", "contents": "Theoretical, practical, and social issues in behavioral treatments of obesity. Although showing superior maintenance, behavioral treatments of obesity typically produce small weight losses at a decelerating rate. Rather than reflecting poor compliance with treatment, these findings are consistent with known compensatory metabolic changes that operate to slow weight loss and promote regain. Other problems associated with dieting include failure of caloric regulation, hyper-responsivity to food palatability, and hunger, which is greater under conditions of moderate restriction and unpredictability of access to food. The inevitability of treatment failure in many instances must be faced and efforts made to prevent further worsening of the obese patient's self-esteem. Prognosis and treatment planning may be aided by consideration of the historical difficulties of weight loss, the degree of hunger experienced on diets, which may reflect important physiological differences among individuals, and the use of food to optimize arousal level. Full involvement of the patient in setting goals and planning treatment is recommended."}
{"id": "PMID:468748", "title": "Behavioral management of exercise: contracting for aerobic points.", "content": "Behavioral contracting was used to encourage physical exercise among college students in a multiple-baseline design. Subjects deposited items of personal value with the experimenters, which they could earn back on fulfillment of two types of contract contingencies. Subjects selected weekly aerobic point criteria, which they could fulfill by exercising in the presence of other subjects. In addition, subjects contracted to observe and record the exercise of other subjects and to perform an independent reliability observation once each week, with both of these activities monitored by the experimenters. Results indicated that the contract contingencies produced increases in the number of aerobic points earned per week for seven of eight subjects, that the aerobic point system possesses several advantages as a dependent variable for behavioral research on exercise, and that inexperienced observers could be quickly trained to observe exercise behavior and to translate those observations into their aerobic point equivalents. Finally, in a followup questionnaire completed 12 months after the end of the study, seven of the eight subjects reported that they were earning more aerobic points per week than had been the case during the baseline condition of this experiment.", "contents": "Behavioral management of exercise: contracting for aerobic points. Behavioral contracting was used to encourage physical exercise among college students in a multiple-baseline design. Subjects deposited items of personal value with the experimenters, which they could earn back on fulfillment of two types of contract contingencies. Subjects selected weekly aerobic point criteria, which they could fulfill by exercising in the presence of other subjects. In addition, subjects contracted to observe and record the exercise of other subjects and to perform an independent reliability observation once each week, with both of these activities monitored by the experimenters. Results indicated that the contract contingencies produced increases in the number of aerobic points earned per week for seven of eight subjects, that the aerobic point system possesses several advantages as a dependent variable for behavioral research on exercise, and that inexperienced observers could be quickly trained to observe exercise behavior and to translate those observations into their aerobic point equivalents. Finally, in a followup questionnaire completed 12 months after the end of the study, seven of the eight subjects reported that they were earning more aerobic points per week than had been the case during the baseline condition of this experiment."}
{"id": "PMID:468749", "title": "A social ecology of hyperactive boys: medication effects in structured classroom environments.", "content": "Hyperactive boys on methylphenidate (Ritalin), hyperactive boys on placebo, and comparison boys were observed in quasi-naturalistic classroom settings. Ambient stimulation (quiet versus noisy conditions) and source of regulation (self-paced versus other-paced activities) were varied in a 2 x 2 design. Compared to their peers, hyperactive boys on placebo showed lower rates of task attention and higher  rates of gross motor movement, regular and negative verbalization, noise-making, physical contact, social initiation, disruption, and acts that were perceived as energetic, inappropriate, or unexpected. Self-paced activities resulted in increased rates of verbalization, social initiation, and high-energy episodes. High ambient noise levels reduced task attention and increased the rates of many other behaviors including verbalization, physical contact, gross motor movement, and high-energy acts. Medication-by-situation interactions emerged for both classroom dimensions, with hyperactive boys on placebo being readily distinguishable from their peers under some classroom conditions and indistinguishable under other conditions. Moderate relationships were found between teacher ratings and many individual behavior categories. Discussion focused on (a) the merits and limitations of a social ecological research perspective, and (b) the implications of these findings for the design of intervention strategies.", "contents": "A social ecology of hyperactive boys: medication effects in structured classroom environments. Hyperactive boys on methylphenidate (Ritalin), hyperactive boys on placebo, and comparison boys were observed in quasi-naturalistic classroom settings. Ambient stimulation (quiet versus noisy conditions) and source of regulation (self-paced versus other-paced activities) were varied in a 2 x 2 design. Compared to their peers, hyperactive boys on placebo showed lower rates of task attention and higher  rates of gross motor movement, regular and negative verbalization, noise-making, physical contact, social initiation, disruption, and acts that were perceived as energetic, inappropriate, or unexpected. Self-paced activities resulted in increased rates of verbalization, social initiation, and high-energy episodes. High ambient noise levels reduced task attention and increased the rates of many other behaviors including verbalization, physical contact, gross motor movement, and high-energy acts. Medication-by-situation interactions emerged for both classroom dimensions, with hyperactive boys on placebo being readily distinguishable from their peers under some classroom conditions and indistinguishable under other conditions. Moderate relationships were found between teacher ratings and many individual behavior categories. Discussion focused on (a) the merits and limitations of a social ecological research perspective, and (b) the implications of these findings for the design of intervention strategies."}
{"id": "PMID:468750", "title": "Behavioral assessment for pediatric intensive care units.", "content": "Two studies were conducted to analyze behaviors of staff and patients on a Pediatric Intensive Care Unit (PICU). In the first study, behavioral observation procedures were employed to assess patient state, physical position, affect, verbal behaviors, visual attention and activity engagement, and staff verbal behavior. On the average, one-third of the patients were judged to be conscious and alert but markedly nonengaged with their environment. In the second study, a member of the hospital staff provided alert patients with individual activities to determine whether a simple environmental manipulation could positively affect behavior of children in intensive care. Employing a reversal design, the activity intervention was found to increase attention and engagement and positive affect, and to decrease inappropriate behavior. Both studies demonstrate that behavioral assessment procedures can provide an empirical basis for designing PICU routines affecting children's psychosocial status, and, thus, complement current procedures designed to provide quality medical care.", "contents": "Behavioral assessment for pediatric intensive care units. Two studies were conducted to analyze behaviors of staff and patients on a Pediatric Intensive Care Unit (PICU). In the first study, behavioral observation procedures were employed to assess patient state, physical position, affect, verbal behaviors, visual attention and activity engagement, and staff verbal behavior. On the average, one-third of the patients were judged to be conscious and alert but markedly nonengaged with their environment. In the second study, a member of the hospital staff provided alert patients with individual activities to determine whether a simple environmental manipulation could positively affect behavior of children in intensive care. Employing a reversal design, the activity intervention was found to increase attention and engagement and positive affect, and to decrease inappropriate behavior. Both studies demonstrate that behavioral assessment procedures can provide an empirical basis for designing PICU routines affecting children's psychosocial status, and, thus, complement current procedures designed to provide quality medical care."}
{"id": "PMID:468751", "title": "Evaluation of biofeedback in the treatment of borderline essential hypertension.", "content": "Direct biofeedback of blood pressure was compared with frontal EMG biofeedback and with self-instructed relaxation for the treatment of essential hypertension in a controlled group outcome study. Patients were followed up for four months after the end of treatment. Generalization of treatment effects was assessed through pre- and posttreatment measurements of blood pressure under clinical conditions in a physician's office. There were no significant reductions in diastolic blood pressure. The systolic blood pressure (SBP) of the patients receiving blood pressure biofeedback decreased 8.1 mm mercury (p = 0.07) and the SBP of the patients in the relaxation condition decreased 9.5 mm mercury (p = 0.05). In the generalization measures, there were significant reductions in SBP for the relaxation group. The results are discussed in terms of the general lack of replicability within the area of biofeedback treatment of hypertension.", "contents": "Evaluation of biofeedback in the treatment of borderline essential hypertension. Direct biofeedback of blood pressure was compared with frontal EMG biofeedback and with self-instructed relaxation for the treatment of essential hypertension in a controlled group outcome study. Patients were followed up for four months after the end of treatment. Generalization of treatment effects was assessed through pre- and posttreatment measurements of blood pressure under clinical conditions in a physician's office. There were no significant reductions in diastolic blood pressure. The systolic blood pressure (SBP) of the patients receiving blood pressure biofeedback decreased 8.1 mm mercury (p = 0.07) and the SBP of the patients in the relaxation condition decreased 9.5 mm mercury (p = 0.05). In the generalization measures, there were significant reductions in SBP for the relaxation group. The results are discussed in terms of the general lack of replicability within the area of biofeedback treatment of hypertension."}
{"id": "PMID:468756", "title": "Personality, body weight and ultimate outcome in anorexia nervosa.", "content": "The Eysenck Personality Inventory, a highly standardized self-rating questionnaire, allows 2 measures of psychosocial functioning on independent scales entitled \"emotionally\" and \"extroversion/introversion.\" A population of severely ill, low body weight anorectics revealed a high degree of \"introversion\" and variable amounts of \"emotionality\" on these measures. Low amounts of \"emotionality\" were associated with abnormally high \"lie\" scores. Following restoration of body weight to full matched population mean levels within an intensive treatment program, significant changes occurred on these measures--increased extroversion scores, decreased emotionality and lie scores. However, possibly qualitative changes had occurred in the state of emotionality since high levels were now related to good clinical outcome many years later. The nature of this phenomenon is discussed.", "contents": "Personality, body weight and ultimate outcome in anorexia nervosa. The Eysenck Personality Inventory, a highly standardized self-rating questionnaire, allows 2 measures of psychosocial functioning on independent scales entitled \"emotionally\" and \"extroversion/introversion.\" A population of severely ill, low body weight anorectics revealed a high degree of \"introversion\" and variable amounts of \"emotionality\" on these measures. Low amounts of \"emotionality\" were associated with abnormally high \"lie\" scores. Following restoration of body weight to full matched population mean levels within an intensive treatment program, significant changes occurred on these measures--increased extroversion scores, decreased emotionality and lie scores. However, possibly qualitative changes had occurred in the state of emotionality since high levels were now related to good clinical outcome many years later. The nature of this phenomenon is discussed."}
{"id": "PMID:468757", "title": "Racing thoughts in depressed patients.", "content": "Patients with racing thoughts and depression may have atypical features that suggest a schizophrenic or borderline state and make diagnosis problematic; clinical management may be difficult because of failure to respond to standard treatments. These patients may have variants of affective illness; and may respond favorably to lithium carbonate.", "contents": "Racing thoughts in depressed patients. Patients with racing thoughts and depression may have atypical features that suggest a schizophrenic or borderline state and make diagnosis problematic; clinical management may be difficult because of failure to respond to standard treatments. These patients may have variants of affective illness; and may respond favorably to lithium carbonate."}
{"id": "PMID:468758", "title": "Psychiatric illness in a general urban emergency room: daytime versus nighttime population.", "content": "Two hundred adults presenting to the emergency room of an urban general hospital were interviewed by a standardized technique to evaluate the existence of a current or previous psychiatric illness. Half of the subjects presented between 8:00 a.m. and 4:00 p.m. (daytime group), and 100 presented between 12:00 a.m. and 8:00 a.m. (nighttime group). In the nighttime population 65% were judged to have current or past psychiatric illnesses. In the daytime population only 36% had current or past psychiatric illnesses. The differences between the 2 populations was highly significant. Fewer than 10% of the 200 patients presented with neuropsychiatric symptoms in their chief complaints.", "contents": "Psychiatric illness in a general urban emergency room: daytime versus nighttime population. Two hundred adults presenting to the emergency room of an urban general hospital were interviewed by a standardized technique to evaluate the existence of a current or previous psychiatric illness. Half of the subjects presented between 8:00 a.m. and 4:00 p.m. (daytime group), and 100 presented between 12:00 a.m. and 8:00 a.m. (nighttime group). In the nighttime population 65% were judged to have current or past psychiatric illnesses. In the daytime population only 36% had current or past psychiatric illnesses. The differences between the 2 populations was highly significant. Fewer than 10% of the 200 patients presented with neuropsychiatric symptoms in their chief complaints."}
{"id": "PMID:468759", "title": "Correlates of clinicians' perceptions of patients in alcoholism treatment.", "content": "Previous research has shown that clinicians' perceptions of alcoholic patients' interpersonal attractiveness exert an influence on clinical decision processes. The present study investigated the relationship between clinicians' perceptions of alcoholic patients, as measured by Byrne's Interpersonal Judgment Scale, and patients' personality, cognitive, and demographic characteristics. The results suggest that the male alcoholic patient is more likely to be perceived favorably if he is young and exhibits behaviors that correspond to clinicians' treatment expectations. Overall, the measured personality characteristics of patients accounted for only small proportions of the variance in clinician's ratings of patients attractiveness. Alternative interpretations of these data are discussed with reference to subsequent research efforts.", "contents": "Correlates of clinicians' perceptions of patients in alcoholism treatment. Previous research has shown that clinicians' perceptions of alcoholic patients' interpersonal attractiveness exert an influence on clinical decision processes. The present study investigated the relationship between clinicians' perceptions of alcoholic patients, as measured by Byrne's Interpersonal Judgment Scale, and patients' personality, cognitive, and demographic characteristics. The results suggest that the male alcoholic patient is more likely to be perceived favorably if he is young and exhibits behaviors that correspond to clinicians' treatment expectations. Overall, the measured personality characteristics of patients accounted for only small proportions of the variance in clinician's ratings of patients attractiveness. Alternative interpretations of these data are discussed with reference to subsequent research efforts."}
{"id": "PMID:468760", "title": "Female orgasm: role of pubococcygeus muscle.", "content": "An examination is made of the role of the pubococcygeus muscle in relation to female orgasm in 281 women. A statistically significant difference is reported between orgasmic and anorgasmic women and the physiological state of the pubococcygeus muscle as measured using a pressure sensitive device inserted in the vagina. These data suggest the pubococcygeus muscle plays an important part in the pathophysiology of female orgasm.", "contents": "Female orgasm: role of pubococcygeus muscle. An examination is made of the role of the pubococcygeus muscle in relation to female orgasm in 281 women. A statistically significant difference is reported between orgasmic and anorgasmic women and the physiological state of the pubococcygeus muscle as measured using a pressure sensitive device inserted in the vagina. These data suggest the pubococcygeus muscle plays an important part in the pathophysiology of female orgasm."}
{"id": "PMID:468761", "title": "Post-parathyroidectomy psychosis: clinical and research implications.", "content": "The case of a 52-year-old female who suffered a post-parathyroidectomy pyschosis is presented and similar cases from the literature are reviewed. Studies of calcium and mangnesium metabolism in mental illness are reviewed, as fluctuations of these ions are felt to be involved in the pathogenesis of this condition. A definable syndrome of post-parathyroidectomy psychosis may exist and the implications of such a syndrome are discussed.", "contents": "Post-parathyroidectomy psychosis: clinical and research implications. The case of a 52-year-old female who suffered a post-parathyroidectomy pyschosis is presented and similar cases from the literature are reviewed. Studies of calcium and mangnesium metabolism in mental illness are reviewed, as fluctuations of these ions are felt to be involved in the pathogenesis of this condition. A definable syndrome of post-parathyroidectomy psychosis may exist and the implications of such a syndrome are discussed."}
{"id": "PMID:468762", "title": "Hyperaldosteronism (Conn's disease) presenting as depression.", "content": "A case of muscle weakness with hypertension is presented. The patient had symptoms of depression. Diagnosis of hyperaldosteronism was suspected because of a low serum potassium and confirmed by discovery of an adrenal adenoma. The role of hypokalemia in mental disturbances is reviewed. Emphasis is placed on possible metabolic etiologies when mood changes, muscle paresis and hypertension coexist.", "contents": "Hyperaldosteronism (Conn's disease) presenting as depression. A case of muscle weakness with hypertension is presented. The patient had symptoms of depression. Diagnosis of hyperaldosteronism was suspected because of a low serum potassium and confirmed by discovery of an adrenal adenoma. The role of hypokalemia in mental disturbances is reviewed. Emphasis is placed on possible metabolic etiologies when mood changes, muscle paresis and hypertension coexist."}
{"id": "PMID:468763", "title": "MMPI and drug treatment in alcohol withdrawal.", "content": "Thirty-four inpatients in a mild phase of alcohol withdrawal, exhibiting anxious and depressive symptoms, were administered an MMPI before and immediately after a 4-week drug treatment period. A double-blind design was used; 17 patients were treated with chlordiazepoxide and 17, with thioridazine. The pretreatment MMPI profile was typical of alcoholic patients; namely, peaks on scales 4 and 2. After the fourth week, the MMPI indicated that thioridazine was significantly more effective than chlordizaepoxide. Decreases in 7 of the 10 clinical scales averaged 7.29 T-score points, whereas only scale 6 was significantly decreased for the patients treated with chlordiazepoxide.", "contents": "MMPI and drug treatment in alcohol withdrawal. Thirty-four inpatients in a mild phase of alcohol withdrawal, exhibiting anxious and depressive symptoms, were administered an MMPI before and immediately after a 4-week drug treatment period. A double-blind design was used; 17 patients were treated with chlordiazepoxide and 17, with thioridazine. The pretreatment MMPI profile was typical of alcoholic patients; namely, peaks on scales 4 and 2. After the fourth week, the MMPI indicated that thioridazine was significantly more effective than chlordizaepoxide. Decreases in 7 of the 10 clinical scales averaged 7.29 T-score points, whereas only scale 6 was significantly decreased for the patients treated with chlordiazepoxide."}
{"id": "PMID:468766", "title": "Detection of gastrin-specific mRNA using oligodeoxynucleotide probes of defined sequence.", "content": "Three oligodeoxynucleotides have been chemically synthesized and used as hybridization probes to detect gastrin-specific mRNA within an heterogeneous population of RNA molecules. Using these oligonucleotides whose nucleotide sequences were deduced from the amino acid sequence of the hormone, 0.2 fmol of gastrin mRNA can be detected/microgram of poly(A)-RNA from hog antrums. The 32P-labeled oligonucleotides hybridize specifically to RNA covalently coupled to DBM-paper (Alwine, J.C., Kemp, D.J., and Stark, G.R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5350-5354). Labeled oligonucleotides also hybridize specifically to RNA separated by gel electrophoresis in the presence of CH3HgOH and covalently coupled to DBM-paper. Using this procedure, we have found that the mRNA coding for gastrin contains about 620 nucleotides, which is in agreement with results obtained using gastrin cDNA to determine mRNA size.", "contents": "Detection of gastrin-specific mRNA using oligodeoxynucleotide probes of defined sequence. Three oligodeoxynucleotides have been chemically synthesized and used as hybridization probes to detect gastrin-specific mRNA within an heterogeneous population of RNA molecules. Using these oligonucleotides whose nucleotide sequences were deduced from the amino acid sequence of the hormone, 0.2 fmol of gastrin mRNA can be detected/microgram of poly(A)-RNA from hog antrums. The 32P-labeled oligonucleotides hybridize specifically to RNA covalently coupled to DBM-paper (Alwine, J.C., Kemp, D.J., and Stark, G.R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5350-5354). Labeled oligonucleotides also hybridize specifically to RNA separated by gel electrophoresis in the presence of CH3HgOH and covalently coupled to DBM-paper. Using this procedure, we have found that the mRNA coding for gastrin contains about 620 nucleotides, which is in agreement with results obtained using gastrin cDNA to determine mRNA size."}
{"id": "PMID:468767", "title": "A receptor-like binding macromolecule for 1 alpha, 25-dihydroxycholecalciferol in cultured mouse bone cells.", "content": "1alpha, 25-Dihydroxycholecalciferol (1,25-(OH)2D3), the active form of vitamin D, like other steroid hormones, initiates its action by binding to cytoplasmic receptors in target cells. Although the 1,25-(OH)2D3 receptor has been well studied in intestine, little information beyond sucrose gradient analyses is presently available from mammalian bone. We, therefore, employed primary cultures of mouse calvarial cells to characterize the mammalian receptor in bone. A hypertonic molybdate-containing buffer was found to protect receptor binding. On hypertonic sucrose gradients, the 1,25-(OH)2-[3H]D3 binder sedimented at 3.2 S. Scatchard analysis of specific 1,25-(OH)2[3H]D3 binding sites at 0 degrees C yielded an apparent Kd of 0.26 nM and an Nmax of 75 fmol/mg of cytosol protein. Competitive binding experiments revealed the receptor to prefer 1,25-(OH)2D3 greater than 25-(OH)-D3 = 1 alpha-(OH)-D3 greater than 24R,25-(OH)2D3; vitamin D3, dihydrotachysterol, sex steroids, and glucocorticoids exhibited negligible binding. As shown in other systems, the receptor could be distinguished from a 25-(OH)-[3H]D3 binder which sedimented at approximately 6 S. In summary, cultured mouse calvarial cells possess a macromolecule with receptor-like properties. This system appears to be an ideal model for the investigation of 1,25-(OH)2D3 receptor binding and action in mammalian bone.", "contents": "A receptor-like binding macromolecule for 1 alpha, 25-dihydroxycholecalciferol in cultured mouse bone cells. 1alpha, 25-Dihydroxycholecalciferol (1,25-(OH)2D3), the active form of vitamin D, like other steroid hormones, initiates its action by binding to cytoplasmic receptors in target cells. Although the 1,25-(OH)2D3 receptor has been well studied in intestine, little information beyond sucrose gradient analyses is presently available from mammalian bone. We, therefore, employed primary cultures of mouse calvarial cells to characterize the mammalian receptor in bone. A hypertonic molybdate-containing buffer was found to protect receptor binding. On hypertonic sucrose gradients, the 1,25-(OH)2-[3H]D3 binder sedimented at 3.2 S. Scatchard analysis of specific 1,25-(OH)2[3H]D3 binding sites at 0 degrees C yielded an apparent Kd of 0.26 nM and an Nmax of 75 fmol/mg of cytosol protein. Competitive binding experiments revealed the receptor to prefer 1,25-(OH)2D3 greater than 25-(OH)-D3 = 1 alpha-(OH)-D3 greater than 24R,25-(OH)2D3; vitamin D3, dihydrotachysterol, sex steroids, and glucocorticoids exhibited negligible binding. As shown in other systems, the receptor could be distinguished from a 25-(OH)-[3H]D3 binder which sedimented at approximately 6 S. In summary, cultured mouse calvarial cells possess a macromolecule with receptor-like properties. This system appears to be an ideal model for the investigation of 1,25-(OH)2D3 receptor binding and action in mammalian bone."}
{"id": "PMID:468768", "title": "The reduction of methemerythrin by e-aq and CO2- from pulse radiolysis studies.", "content": "The rate constants for reduction of methemerythrin from Phascolopsis gouldii and Themiste pyroides by hydrated electrons are 2.0 and 3.9 x 10(9) M(-1)s(-1), respectively, at pH 8.2, I = 0.03 M, and 25 degrees C. There is only a small increase in rate when the pH is lowered to 6.3 and a very small decrease when the ionic strength is raised to 0.1 M. Adding azide ion (to form the met-azide adduct) has little effect on the reactivity towards e-aq. For the monomer form, metmyohemerythrin from T. pyroides, the reaction rate constant is 4.5 x 10(9) M(-1)s(-1). Methemerythrin from T. pyroides reacts with CO2- with a rate constant 6.8 x 10(7) M(-1)s(-1). The reactivity sequence e-aq greater than CO2- greater than SO2- (from dithionite reduction) towards methemerythrin is the same as that observed with reduction of heme proteins but the rate constants are some 10 to 100 times smaller for the former. Only 10 to 20% of the e-aq or CO2- radicals generated effect reduction of the iron centers in methemerythrin.", "contents": "The reduction of methemerythrin by e-aq and CO2- from pulse radiolysis studies. The rate constants for reduction of methemerythrin from Phascolopsis gouldii and Themiste pyroides by hydrated electrons are 2.0 and 3.9 x 10(9) M(-1)s(-1), respectively, at pH 8.2, I = 0.03 M, and 25 degrees C. There is only a small increase in rate when the pH is lowered to 6.3 and a very small decrease when the ionic strength is raised to 0.1 M. Adding azide ion (to form the met-azide adduct) has little effect on the reactivity towards e-aq. For the monomer form, metmyohemerythrin from T. pyroides, the reaction rate constant is 4.5 x 10(9) M(-1)s(-1). Methemerythrin from T. pyroides reacts with CO2- with a rate constant 6.8 x 10(7) M(-1)s(-1). The reactivity sequence e-aq greater than CO2- greater than SO2- (from dithionite reduction) towards methemerythrin is the same as that observed with reduction of heme proteins but the rate constants are some 10 to 100 times smaller for the former. Only 10 to 20% of the e-aq or CO2- radicals generated effect reduction of the iron centers in methemerythrin."}
{"id": "PMID:468769", "title": "Separation and properties of five glycosaminoglycan sulfatases from rat skin.", "content": "Chondroitin sulfates, dermatan sulfate, heparan sulfate, heparin, keratan sulfate, and oligosaccharides derived from these sulfated glycosaminoglycans have been used for the measurement of sulfatase activity of rat skin extracts. Chromatographic fractionation of the extracts followed by specificity studies demonstrated the existence of five different sulfatases, specific for 1) the nonreducing N-acetylglucosamine 6-sulfate end groups of heparin sulfate and keratan sulfate, 2) the nonreducing N-acetylgalactosamine (or galactose) 6-sulfate end groups of chondroitin sulfate (or keratan sulfate), 3) the nonreducing N-acetylgalactosamine 4-sulfate end groups of chondroitin sulfate and dermatan sulfate, 4) certain suitably located glucosamine N-sulfate groups of heparin and heparan sulfate, or 5) certain suitably located iduronate sulfate groups of heparan sulfate and dermatan sulfate. Two arylsulfatases, one of which was identical in its chromatographic behaviors with the third enzyme described above, were also demonstrated in the extracts. These results taken together with those previously obtained from studies on human fibroblast cultures suggest that normal skin fibroblasts contain at least five specific sulfatases and diminished activity of any one may result in a specific storage disease.", "contents": "Separation and properties of five glycosaminoglycan sulfatases from rat skin. Chondroitin sulfates, dermatan sulfate, heparan sulfate, heparin, keratan sulfate, and oligosaccharides derived from these sulfated glycosaminoglycans have been used for the measurement of sulfatase activity of rat skin extracts. Chromatographic fractionation of the extracts followed by specificity studies demonstrated the existence of five different sulfatases, specific for 1) the nonreducing N-acetylglucosamine 6-sulfate end groups of heparin sulfate and keratan sulfate, 2) the nonreducing N-acetylgalactosamine (or galactose) 6-sulfate end groups of chondroitin sulfate (or keratan sulfate), 3) the nonreducing N-acetylgalactosamine 4-sulfate end groups of chondroitin sulfate and dermatan sulfate, 4) certain suitably located glucosamine N-sulfate groups of heparin and heparan sulfate, or 5) certain suitably located iduronate sulfate groups of heparan sulfate and dermatan sulfate. Two arylsulfatases, one of which was identical in its chromatographic behaviors with the third enzyme described above, were also demonstrated in the extracts. These results taken together with those previously obtained from studies on human fibroblast cultures suggest that normal skin fibroblasts contain at least five specific sulfatases and diminished activity of any one may result in a specific storage disease."}
{"id": "PMID:468772", "title": "The structure of mouse testicular lactate dehydrogenase isoenzyme C4 at 2.9 A resolution.", "content": "The structure of lactate dehydrogenase isoenzyme C4 from mouse testes was solved at 2.9 A resolution using the technique of molecular replacement. The electron density map revealed a ternary-like configuration of the flexible loop peptide although density corresponding to the coenzyme and substrate molecules was not present. Apparently the apo-lactate dehydrogenase molecule in solution is in a dynamic equilibrium between the O (loop open as found in dogfish apo-lactate dehydrogenase M4) and C (loop closed as found in a variety of ternary complexes) conformations. During crystallization of the apoenzyme one or the other conformers is selected. The apparent stability of the closed conformation for the apo-lactate dehydrogenase C4 molecule may in part explain the low catalytic turnover number of the C isoenzyme. A possible substitution of an arginine residue at position 30 may also be a contributing factor as well as allowing NADP to act as coenzyme.", "contents": "The structure of mouse testicular lactate dehydrogenase isoenzyme C4 at 2.9 A resolution. The structure of lactate dehydrogenase isoenzyme C4 from mouse testes was solved at 2.9 A resolution using the technique of molecular replacement. The electron density map revealed a ternary-like configuration of the flexible loop peptide although density corresponding to the coenzyme and substrate molecules was not present. Apparently the apo-lactate dehydrogenase molecule in solution is in a dynamic equilibrium between the O (loop open as found in dogfish apo-lactate dehydrogenase M4) and C (loop closed as found in a variety of ternary complexes) conformations. During crystallization of the apoenzyme one or the other conformers is selected. The apparent stability of the closed conformation for the apo-lactate dehydrogenase C4 molecule may in part explain the low catalytic turnover number of the C isoenzyme. A possible substitution of an arginine residue at position 30 may also be a contributing factor as well as allowing NADP to act as coenzyme."}
{"id": "PMID:468774", "title": "The tentative amino acid sequencing of lactate dehydrogenase C4 by X-ray diffraction analysis.", "content": "A tentative amino acid sequence of mouse testicular lactate dehydrogenase C4 was deduced from an electron density map and comparison with five other known lactate dehydrogenase sequences. The amino acid composition determined by chemical analysis agrees reasonably well with the present results. Necessary changes in amino acids were largely conservative and confined to the external portions of the molecule. Residues in the Q and P subunit contact regions were particularly well conserved as were most internal residues. The minimum base change/codon was similar between the C and H isoenzymes and between the C and M isoenzymes.", "contents": "The tentative amino acid sequencing of lactate dehydrogenase C4 by X-ray diffraction analysis. A tentative amino acid sequence of mouse testicular lactate dehydrogenase C4 was deduced from an electron density map and comparison with five other known lactate dehydrogenase sequences. The amino acid composition determined by chemical analysis agrees reasonably well with the present results. Necessary changes in amino acids were largely conservative and confined to the external portions of the molecule. Residues in the Q and P subunit contact regions were particularly well conserved as were most internal residues. The minimum base change/codon was similar between the C and H isoenzymes and between the C and M isoenzymes."}
{"id": "PMID:468776", "title": "Lima bean proteinase inhibitor. Origins of circular dichroism bands and modification by Br2- and (CNS)2-.", "content": "Origins of CD bands in lima bean proteinase inhibitor were deduced from an acetylation-deacetylation study of the sole tyrosyl residue in the protein (Tyr 69), and by analogy with Bowman-Birk soybean proteinase inhibitor, a homologous protein with similar spectral properties. Tyr 69 is relatively inaccessible to N-acetylimidazole; 100-fold molar excess of the reagent in the presence of 6 M guanidine hydrochloride elicited about 70 to 80% O-acetylation. A broad negative CD band centered around 280 nm arises mainly from the longest wavelength transition of cystinyl side chains (epsilon L--epsilon R approximately equal to -0.8 M-1 cm-1 per disulfide). The second cystinyl transition gives rise to a positive CD band of a comparable intensity at 247 nm. The Lb vibronic transition of Tyr 69 has negative CD around 280 nm, contributing approximately 10% of the total CD intensity at 278 nm (epsilon L--epsilon R approximately equal to -0.5 M-1 cm-1). The 232 nm positive shoulder is from the La vibronic transition of Tyr 69. Radical anions, Br2- and (CNS)2-, generated by the irradiation of N2O-saturated inhibitor solutions containing KBr or KCNS, reduced tyrosyl CD without affecting disulfide CD bands, indicating that the radical anions damaged Tyr 69 without altering protein conformation. The inhibitor modified at Tyr 69 by Br2- and (CNS)2- retained full activity toward trypsin and chymotrypsin. The irradiation of the inhibitor in the air-saturated solution led to loss in tyrosyl as well as cystinyl CD bands and decline in both antiproteinase activities.", "contents": "Lima bean proteinase inhibitor. Origins of circular dichroism bands and modification by Br2- and (CNS)2-. Origins of CD bands in lima bean proteinase inhibitor were deduced from an acetylation-deacetylation study of the sole tyrosyl residue in the protein (Tyr 69), and by analogy with Bowman-Birk soybean proteinase inhibitor, a homologous protein with similar spectral properties. Tyr 69 is relatively inaccessible to N-acetylimidazole; 100-fold molar excess of the reagent in the presence of 6 M guanidine hydrochloride elicited about 70 to 80% O-acetylation. A broad negative CD band centered around 280 nm arises mainly from the longest wavelength transition of cystinyl side chains (epsilon L--epsilon R approximately equal to -0.8 M-1 cm-1 per disulfide). The second cystinyl transition gives rise to a positive CD band of a comparable intensity at 247 nm. The Lb vibronic transition of Tyr 69 has negative CD around 280 nm, contributing approximately 10% of the total CD intensity at 278 nm (epsilon L--epsilon R approximately equal to -0.5 M-1 cm-1). The 232 nm positive shoulder is from the La vibronic transition of Tyr 69. Radical anions, Br2- and (CNS)2-, generated by the irradiation of N2O-saturated inhibitor solutions containing KBr or KCNS, reduced tyrosyl CD without affecting disulfide CD bands, indicating that the radical anions damaged Tyr 69 without altering protein conformation. The inhibitor modified at Tyr 69 by Br2- and (CNS)2- retained full activity toward trypsin and chymotrypsin. The irradiation of the inhibitor in the air-saturated solution led to loss in tyrosyl as well as cystinyl CD bands and decline in both antiproteinase activities."}
{"id": "PMID:468778", "title": "Purification and chemical characterization of papain-solubilized histocompatibility-2 antigens from mouse liver.", "content": "A large scale purification of histocompatibility-2 (H-2) antigens from mouse liver is described. The antigens were solubilized by a limited papain digestion of a crude preparation of liver membranes (strain A/J) and purified by ion exchange chromatography, gel filtration, affinity chromatography, and isoelectric focusing. The overall degree of purification of H-2Kk was 1,300-fold and that of H-2Dd was 1,500-fold; approximately 8 mg of purified H-2a antigens were obtained from 1 kg of liver. The purification was followed by a sensitive radioimmunoassay in which H-2a-containing fractions were used to inhibit the binding of 125I-labeled H-2a to appropriate antisera. H-2Dd and H-2Kk co-purified through all the steps but the concentration of H-2Kk was 2- to 3-fold higher than that of H-2Dd in the liver homogenate as well as in the purified H-2 preparation. beta 2-microglobulin was initially present in a 3- to 10-fold excess over H-2 in the liver homogenate, but the purified H-2 preparation contained approximately 2 mol of alloantigenic heavy chain/mol of beta 2-microglobulin. Isoelectric focusing and disc-gel electrophoresis showed a charge heterogeneity of H-2, with a mean isoelectric point of pH 4.9. Electrophoresis on sodium dodecyl sulfate gels showed one band. Denaturing conditions were required to remove beta 2-microglobulin and small amounts of impurities from H-2. The amino acid sequence of the first 27 residues of the isolated heavy chains was determined.", "contents": "Purification and chemical characterization of papain-solubilized histocompatibility-2 antigens from mouse liver. A large scale purification of histocompatibility-2 (H-2) antigens from mouse liver is described. The antigens were solubilized by a limited papain digestion of a crude preparation of liver membranes (strain A/J) and purified by ion exchange chromatography, gel filtration, affinity chromatography, and isoelectric focusing. The overall degree of purification of H-2Kk was 1,300-fold and that of H-2Dd was 1,500-fold; approximately 8 mg of purified H-2a antigens were obtained from 1 kg of liver. The purification was followed by a sensitive radioimmunoassay in which H-2a-containing fractions were used to inhibit the binding of 125I-labeled H-2a to appropriate antisera. H-2Dd and H-2Kk co-purified through all the steps but the concentration of H-2Kk was 2- to 3-fold higher than that of H-2Dd in the liver homogenate as well as in the purified H-2 preparation. beta 2-microglobulin was initially present in a 3- to 10-fold excess over H-2 in the liver homogenate, but the purified H-2 preparation contained approximately 2 mol of alloantigenic heavy chain/mol of beta 2-microglobulin. Isoelectric focusing and disc-gel electrophoresis showed a charge heterogeneity of H-2, with a mean isoelectric point of pH 4.9. Electrophoresis on sodium dodecyl sulfate gels showed one band. Denaturing conditions were required to remove beta 2-microglobulin and small amounts of impurities from H-2. The amino acid sequence of the first 27 residues of the isolated heavy chains was determined."}
{"id": "PMID:468782", "title": "Role of eukaryotic initiation factor 5 in the formation of 80 S initiation complexes.", "content": "The function of eukaryotic initiation factor 5 (eIF-5) from rabbit reticulocyte lysate has been studied by sucrose gradient preparation of 40 S and 80 S initiation complexes. eIF-5 is required for transfer of initiator tRNA from 40 S preinitiation complexes to puromycin-reactive 80 S complexes. The transfer is dependent upon GTP hydrolysis and is associated with release of eIF-2 and eIF-3 from the 40 S subunit. The GTP-dependent loss of eIF-2 and eIF-3 is catalyzed by eIF-5 in the absence of 60 S subunits or when subunit joining is prevented by edeine, but not when GTP is replaced by GuoPP(NH)P. Unstable 40 S subunit . Met-tRNAf complexes generated by eIF-5 can form puromycin-reactive 80 S complexes when 60 S subunits are added in the absence of added GTP. In addition, kinetic evidence is presented that indicates GTP hydrolysis occurs prior to 80 S complex formation.", "contents": "Role of eukaryotic initiation factor 5 in the formation of 80 S initiation complexes. The function of eukaryotic initiation factor 5 (eIF-5) from rabbit reticulocyte lysate has been studied by sucrose gradient preparation of 40 S and 80 S initiation complexes. eIF-5 is required for transfer of initiator tRNA from 40 S preinitiation complexes to puromycin-reactive 80 S complexes. The transfer is dependent upon GTP hydrolysis and is associated with release of eIF-2 and eIF-3 from the 40 S subunit. The GTP-dependent loss of eIF-2 and eIF-3 is catalyzed by eIF-5 in the absence of 60 S subunits or when subunit joining is prevented by edeine, but not when GTP is replaced by GuoPP(NH)P. Unstable 40 S subunit . Met-tRNAf complexes generated by eIF-5 can form puromycin-reactive 80 S complexes when 60 S subunits are added in the absence of added GTP. In addition, kinetic evidence is presented that indicates GTP hydrolysis occurs prior to 80 S complex formation."}
{"id": "PMID:468783", "title": "Thermodynamics of the interaction of insulin with its receptor.", "content": "Insulin binding to its cellular receptors is markedly dependent on the temperature. The thermodynamic parameters for the reaction of insulin with the high affinity state of its receptor have been evaluated with equilibrium studies at multiple temperatures between 5 degrees and 37 degrees C. The thermodynamics of the insulin-receptor interaction is not classical. The van't Hoff plot is not linear. Both the enthalpy and entropy changes, due to the formation of the hormone . receptor complex, decrease markedly with temperature, corresponding to a large heat capacity change of -766 cal/(mol deg) at 25 degrees C. The reaction is endothermic and entropically driven at low temperature and exothermic and enthalpically driven at higher temperature. This thermodynamic behavior is suggestive of a hydrophobic reaction and supports Blundell's concept that the loss of non-polar surface residues in the formation of the hormone . receptor complex is an important driving force of the reaction. Alternatively, this nonclassical behavior may indicate that the reaction of insulin with its receptor involves more than one step.", "contents": "Thermodynamics of the interaction of insulin with its receptor. Insulin binding to its cellular receptors is markedly dependent on the temperature. The thermodynamic parameters for the reaction of insulin with the high affinity state of its receptor have been evaluated with equilibrium studies at multiple temperatures between 5 degrees and 37 degrees C. The thermodynamics of the insulin-receptor interaction is not classical. The van't Hoff plot is not linear. Both the enthalpy and entropy changes, due to the formation of the hormone . receptor complex, decrease markedly with temperature, corresponding to a large heat capacity change of -766 cal/(mol deg) at 25 degrees C. The reaction is endothermic and entropically driven at low temperature and exothermic and enthalpically driven at higher temperature. This thermodynamic behavior is suggestive of a hydrophobic reaction and supports Blundell's concept that the loss of non-polar surface residues in the formation of the hormone . receptor complex is an important driving force of the reaction. Alternatively, this nonclassical behavior may indicate that the reaction of insulin with its receptor involves more than one step."}
{"id": "PMID:468784", "title": "Interaction of membranous enzymes with membranous lipid substrates. Hydrolysis of diacylglycerol by lipase in rat brain microsomes.", "content": "The phospholipids in rat brain microsomes were labeled with tritium by intracerebral administration of radioactive fatty acids and converted to diacylglycerol with phospholipase C. The latter lipid was hydrolyzed in situ at pH 4.8, to monoacylglycerol and fatty acid by the endogenous microsomal lipase. This paper provides an experimental approach to determine whether the lipid was degraded by enzyme molecules residing in its own membrane (intramembrane interaction) or an adjacent membrane (intermembrane interaction). Direct interaction between separate membranes containing enzyme or substrate showed the existence of the inter-membrane route while dilution experiments provided evidence for the presence of the intramembrane interaction as well. A probable difference in the mechanisms of these two interactions is suggested by different shapes of the curves that describe the reaction rate as a function of the endogenous substrate. The curve resulting from the intermembrane interaction was hyperbolic while that representing the intramembrane route was of a parabola-like shape. Competition experiments suggested that when given a choice between the two, the enzyme utilized preferentially the substrate molecules in its own membrane.", "contents": "Interaction of membranous enzymes with membranous lipid substrates. Hydrolysis of diacylglycerol by lipase in rat brain microsomes. The phospholipids in rat brain microsomes were labeled with tritium by intracerebral administration of radioactive fatty acids and converted to diacylglycerol with phospholipase C. The latter lipid was hydrolyzed in situ at pH 4.8, to monoacylglycerol and fatty acid by the endogenous microsomal lipase. This paper provides an experimental approach to determine whether the lipid was degraded by enzyme molecules residing in its own membrane (intramembrane interaction) or an adjacent membrane (intermembrane interaction). Direct interaction between separate membranes containing enzyme or substrate showed the existence of the inter-membrane route while dilution experiments provided evidence for the presence of the intramembrane interaction as well. A probable difference in the mechanisms of these two interactions is suggested by different shapes of the curves that describe the reaction rate as a function of the endogenous substrate. The curve resulting from the intermembrane interaction was hyperbolic while that representing the intramembrane route was of a parabola-like shape. Competition experiments suggested that when given a choice between the two, the enzyme utilized preferentially the substrate molecules in its own membrane."}
{"id": "PMID:468788", "title": "Stimulation and inhibition of cellular functions by glucocorticoids. Correlations with rapid influences on chromatin structure.", "content": "The effect of media conditions on the glucocorticoid response has been examined in three types of cultured cells. In rat pituitary tumor cells (GC cells) growth hormone production was stimulated by glucocorticoids provided fresh culture media was present (enriched media conditions). In contrast, dexamethasone either failed to induce or deinduce growth hormone synthesis if added to cultures which had not received fresh media for 3 days (depleted media condition). With human skin fibroblasts, cortisol stimulated [3H]thymidine incorporation in the enriched condition but inhibited this response in the depleted condition. In mouse lymphoma (S49) cells the enriched media conditions significantly delayed the killing response to glucocorticoids (20% killing after 24 h versus 90% killing after 24 h for the depleted condition). Thus, the magnitude and in some cases, the direction of the glucocorticoid response are sensitive to the conditions to which the cells are exposed. In all three cell types the steroid also rapidly (detectable by 15 min, maximal by 2 h) altered chromatin structure as detected by a change in the number of initiation sites for Escherichia coli RNA polymerase assayed under cell-free conditions. This early nuclear response could be in a positive or negative direction and was also affected by the culture conditions; enriched media favored a positive or less negative effect on the initiation sites by the steroid, while depleted media favored a steroid-induced inhibition of this chromatin function. In S49 and GC cells the kinetics and magnitude of the change in chromatin closely followed receptor . glucocorticoid complex binding to nuclei while removal of dexamethasone from the culture media resulted in a rapid (t 1/2 = approximately 20 min) disappearance of the effect which paralleled loss of bound hormone from the nucleus. The glucocorticoid effect on chromatin was not observed in two lines of glucocorticoid-resistant mutant S49 cells. One line (R-) lacks detectable glucocorticoid receptors; the other line (Nti) has receptors that bind the hormone normally, but the receptor . glucocorticoid complexes bind more avidly to the nucleus. These results suggest that the receptor is involved in both the stimulatory and the inhibitory effects on chromatin. The findings in the Nti cells and of a slight lag between nuclear binding of receptors and initiation site alteration implies that some receptor property, in addition to nuclear binding per se, is responsible for the influence on chromatin. These results are discussed in terms of a model in which steroid hormones initiate their actions by influencing a reaction that modifies chromatin structure. The direction and magnitude of the reaction, and its effect on the expression of specific genes, are dictated by the metabolic state and differentiation of the cell.", "contents": "Stimulation and inhibition of cellular functions by glucocorticoids. Correlations with rapid influences on chromatin structure. The effect of media conditions on the glucocorticoid response has been examined in three types of cultured cells. In rat pituitary tumor cells (GC cells) growth hormone production was stimulated by glucocorticoids provided fresh culture media was present (enriched media conditions). In contrast, dexamethasone either failed to induce or deinduce growth hormone synthesis if added to cultures which had not received fresh media for 3 days (depleted media condition). With human skin fibroblasts, cortisol stimulated [3H]thymidine incorporation in the enriched condition but inhibited this response in the depleted condition. In mouse lymphoma (S49) cells the enriched media conditions significantly delayed the killing response to glucocorticoids (20% killing after 24 h versus 90% killing after 24 h for the depleted condition). Thus, the magnitude and in some cases, the direction of the glucocorticoid response are sensitive to the conditions to which the cells are exposed. In all three cell types the steroid also rapidly (detectable by 15 min, maximal by 2 h) altered chromatin structure as detected by a change in the number of initiation sites for Escherichia coli RNA polymerase assayed under cell-free conditions. This early nuclear response could be in a positive or negative direction and was also affected by the culture conditions; enriched media favored a positive or less negative effect on the initiation sites by the steroid, while depleted media favored a steroid-induced inhibition of this chromatin function. In S49 and GC cells the kinetics and magnitude of the change in chromatin closely followed receptor . glucocorticoid complex binding to nuclei while removal of dexamethasone from the culture media resulted in a rapid (t 1/2 = approximately 20 min) disappearance of the effect which paralleled loss of bound hormone from the nucleus. The glucocorticoid effect on chromatin was not observed in two lines of glucocorticoid-resistant mutant S49 cells. One line (R-) lacks detectable glucocorticoid receptors; the other line (Nti) has receptors that bind the hormone normally, but the receptor . glucocorticoid complexes bind more avidly to the nucleus. These results suggest that the receptor is involved in both the stimulatory and the inhibitory effects on chromatin. The findings in the Nti cells and of a slight lag between nuclear binding of receptors and initiation site alteration implies that some receptor property, in addition to nuclear binding per se, is responsible for the influence on chromatin. These results are discussed in terms of a model in which steroid hormones initiate their actions by influencing a reaction that modifies chromatin structure. The direction and magnitude of the reaction, and its effect on the expression of specific genes, are dictated by the metabolic state and differentiation of the cell."}
{"id": "PMID:468789", "title": "Purification and characterization of two phospholipid exchange proteins from bovine heart.", "content": "Two phospholipid exchange proteins from bovine heart have been purified approximately 2000-fold and judged greater than 90% pure. The proteins are similar in molecular weight (both 33,400 by polyacrylamide gel electrophoresis and 23,500 by gel filtration), in amino acid composition, and in specificity, although they differ in isoelectric points, 5.3 and 5.6. The transfer of phospholipids between artificial membranes is catalyzed by these proteins at the following relative rates: 100 for phosphatidylinositol, 35 for phosphatidylcholine, 5 for sphingomyelin, and 0.1 for phosphatidylethanolamine. The use of these exchange proteins in the study of mixed phospholipid vesicle structure is demonstrated. The purified proteins catalyze the substitution of one membrane phospholipid species for another at a rate comparable to true exchange. The phospholipid exchange activity is inhibited by the presence of sphingomyelin, and also by reagents which react with sulfhydryl groups. Evidence is presented for two sites of N-ethylmaleimide binding on these exchange proteins. Reaction with one site has little effect on activity and occurs in the absence of membranes. Reaction with the second site occurs in the presence of phospholipid vesicles and leads to complete, irreversible inhibition of exchange activity.", "contents": "Purification and characterization of two phospholipid exchange proteins from bovine heart. Two phospholipid exchange proteins from bovine heart have been purified approximately 2000-fold and judged greater than 90% pure. The proteins are similar in molecular weight (both 33,400 by polyacrylamide gel electrophoresis and 23,500 by gel filtration), in amino acid composition, and in specificity, although they differ in isoelectric points, 5.3 and 5.6. The transfer of phospholipids between artificial membranes is catalyzed by these proteins at the following relative rates: 100 for phosphatidylinositol, 35 for phosphatidylcholine, 5 for sphingomyelin, and 0.1 for phosphatidylethanolamine. The use of these exchange proteins in the study of mixed phospholipid vesicle structure is demonstrated. The purified proteins catalyze the substitution of one membrane phospholipid species for another at a rate comparable to true exchange. The phospholipid exchange activity is inhibited by the presence of sphingomyelin, and also by reagents which react with sulfhydryl groups. Evidence is presented for two sites of N-ethylmaleimide binding on these exchange proteins. Reaction with one site has little effect on activity and occurs in the absence of membranes. Reaction with the second site occurs in the presence of phospholipid vesicles and leads to complete, irreversible inhibition of exchange activity."}
{"id": "PMID:468791", "title": "Purification and properties of the deoxyribonucleic acid polymerase induced by vaccinia virus.", "content": "The vaccinia virus-induced DNA polymerase has been purified about 500-fold from a cytoplasmic extract of vaccinia-infected HeLa cells. Analysis of the purified fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals a single polypeptide of 110,000 daltons, which is greater than 95% pure. This polypeptide co-sediments with polymerase activity through a glycerol gradient. The sedimentation coefficient of the enzyme is 6.3 S, and its Stokes radius is 4.6 nm. The molecular weight of the native enzyme derived from these values is 115,000. Vaccinia polymerase is therefore a single large polypeptide of 110,000 to 115,000 daltons. The purified fraction has no significant endonuclease activity, but a strong exonuclease activity co-purifies with polymerase activity through every step in the isolation. The polymerase and exonuclease activities are inactivated at 45 degrees C at the same rate. It is likely, therefore, that both activities are catalyzed by the same polypeptide. The exonuclease hydrolyzes DNA predominantly in the 3' leads to 5' direction, to produce 5' mononucleotides. The exonuclease degrades single-stranded DNA more rapidly than duplex DNA, and the rate of digestion of both single-stranded and double-stranded DNA increases as the size of the substrate decreases. Single-stranded circular DNA is a potent inhibitor of the exonuclease activity, but duplex circular DNA has no significant effect on its activity.", "contents": "Purification and properties of the deoxyribonucleic acid polymerase induced by vaccinia virus. The vaccinia virus-induced DNA polymerase has been purified about 500-fold from a cytoplasmic extract of vaccinia-infected HeLa cells. Analysis of the purified fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals a single polypeptide of 110,000 daltons, which is greater than 95% pure. This polypeptide co-sediments with polymerase activity through a glycerol gradient. The sedimentation coefficient of the enzyme is 6.3 S, and its Stokes radius is 4.6 nm. The molecular weight of the native enzyme derived from these values is 115,000. Vaccinia polymerase is therefore a single large polypeptide of 110,000 to 115,000 daltons. The purified fraction has no significant endonuclease activity, but a strong exonuclease activity co-purifies with polymerase activity through every step in the isolation. The polymerase and exonuclease activities are inactivated at 45 degrees C at the same rate. It is likely, therefore, that both activities are catalyzed by the same polypeptide. The exonuclease hydrolyzes DNA predominantly in the 3' leads to 5' direction, to produce 5' mononucleotides. The exonuclease degrades single-stranded DNA more rapidly than duplex DNA, and the rate of digestion of both single-stranded and double-stranded DNA increases as the size of the substrate decreases. Single-stranded circular DNA is a potent inhibitor of the exonuclease activity, but duplex circular DNA has no significant effect on its activity."}
{"id": "PMID:468792", "title": "Action of Arthrobacter ureafaciens sialidase on sialoglycolipid substrates. Mode of action and highly specific recognition of the oligosaccharide moiety of ganglioside GM1.", "content": "A new bacterial sialidase (N-acetylneuraminate glycohydrolase, EC 3.2.1.18) isolated from the culture filtrate of Arthrobacter ureafaciens was characterized in detail with respect to its action on sialoglycolipids. Strong electrolytes had a reversible inhibitory effect on the action of the enzyme on brain gangliosides in accordance with Debye-H\u00fcckel effect of ionic environment on ionic activity, and resulted in an acidic shift and a broadening of the pH optimum. Both ionic and non-ionic detergents markedly enhanced the enzymic activity on the gangliosides, and caused an acidic shift on the pH optimum of this enzyme. Sulfhydryl groups seemed to be involved in its active site. This enzyme had a highly specific action on sialidase-resistant ganglioside GM1, showing about 100-fold higher activity on GM1 than Clostridium perfringens sialidase, the only sialidase so far reported to cleave the lipid substrate in the presence of bile salts. In the absence of detergents, the activity of A. ureafaciens sialidase on GM1 was very low. Ganglioside GM1 in either the monomeric or micelar form was hydrolyzed to asialo-GM1 by A. ureafaciens sialidase most efficiently in the presence of sodium cholate of about three times the GM1 molar concentration. The presence of detergents increased both the Km and Vmax values for ganglioside GM1. The oligosaccharide prepared from GM1 by ozonolysis was cleaved well by this sialidase in the absence of detergents, and no detergent was found to affect the hydrolysis. The Km value for the sugar substrate was about two orders of magnitude greater than that for the corresponding lipid substrate. It is suggested that the hydrophobic ceramide moiety increases affinity of the lipid substrate to the enzyme, but inhibits hydrolysis of the substrate, possibly due to its hydrophobic interaction with hydrophobic portions of the enzyme molecule (resulting in lower Km and Vmax for lipid substrates). This inhibition may be released by detergent due to formation of mixed micelles of sialoglycolipid and detergent molecules. It is also indicated that recognition of the specific saccharide structure of GM1 by individual sialidases is essential for release of the resistant sialyl residue, and that A. ureafaciens sialidase seemed to have an isoenzymic or oligomeric structure.", "contents": "Action of Arthrobacter ureafaciens sialidase on sialoglycolipid substrates. Mode of action and highly specific recognition of the oligosaccharide moiety of ganglioside GM1. A new bacterial sialidase (N-acetylneuraminate glycohydrolase, EC 3.2.1.18) isolated from the culture filtrate of Arthrobacter ureafaciens was characterized in detail with respect to its action on sialoglycolipids. Strong electrolytes had a reversible inhibitory effect on the action of the enzyme on brain gangliosides in accordance with Debye-H\u00fcckel effect of ionic environment on ionic activity, and resulted in an acidic shift and a broadening of the pH optimum. Both ionic and non-ionic detergents markedly enhanced the enzymic activity on the gangliosides, and caused an acidic shift on the pH optimum of this enzyme. Sulfhydryl groups seemed to be involved in its active site. This enzyme had a highly specific action on sialidase-resistant ganglioside GM1, showing about 100-fold higher activity on GM1 than Clostridium perfringens sialidase, the only sialidase so far reported to cleave the lipid substrate in the presence of bile salts. In the absence of detergents, the activity of A. ureafaciens sialidase on GM1 was very low. Ganglioside GM1 in either the monomeric or micelar form was hydrolyzed to asialo-GM1 by A. ureafaciens sialidase most efficiently in the presence of sodium cholate of about three times the GM1 molar concentration. The presence of detergents increased both the Km and Vmax values for ganglioside GM1. The oligosaccharide prepared from GM1 by ozonolysis was cleaved well by this sialidase in the absence of detergents, and no detergent was found to affect the hydrolysis. The Km value for the sugar substrate was about two orders of magnitude greater than that for the corresponding lipid substrate. It is suggested that the hydrophobic ceramide moiety increases affinity of the lipid substrate to the enzyme, but inhibits hydrolysis of the substrate, possibly due to its hydrophobic interaction with hydrophobic portions of the enzyme molecule (resulting in lower Km and Vmax for lipid substrates). This inhibition may be released by detergent due to formation of mixed micelles of sialoglycolipid and detergent molecules. It is also indicated that recognition of the specific saccharide structure of GM1 by individual sialidases is essential for release of the resistant sialyl residue, and that A. ureafaciens sialidase seemed to have an isoenzymic or oligomeric structure."}
{"id": "PMID:468793", "title": "Modulation of the phosphorylation state of rat liver pyruvate kinase by allosteric effectors and insulin.", "content": "The regulation of pyruvate kinase in isolated hepatocytes from fasted rats was studied where the intracellular level of fructose 1,6-bisphosphate was elevated 5-fold by the addition of 5 mM dihydroxyacetone. In this case, flux through pyruvate kinase was increased. The increase in flux correlated with an elevation in fructose bisphosphate levels but not with P-enolpyruvate levels which were unchanged. Pyruvate kinase was activated and its affinity for P-enolpyruvate was increased 7-fold in hepatocyte homogenates. Precipitation of the enzyme from homogenates with ammonium sulfate removed fructose 1,6-bisphosphate and activation was no longer observed. These results indicate that flux through and activity of pyruvate kinase can be controlled by the intracellular level of fructose 1,6-bisphosphate. The effect of elevated fructose 1,6-bisphosphate levels on the ability of glucagon to inactivate pyruvate kinase was also studied where only covalent enzyme modification is observed. Inactivation by maximally effective hormone concentrations was unaffected by elevated levels of fructose 1,6-bisphosphate, but the half-maximally effective concentration was increased from 0.3 to 0.8 nM. Activation of the cyclic AMP-dependent protein kinase by 0.3 nM glucagon was unaffected, but the initial rate of pyruvate kinase inactivation was suppressed. These results suggest that alterations in the level of fructose 1,6-bisphosphate can affect the ability of physiological concentrations of glucagon to inactivate pyruvate kinase by opposing phosphorylation of the enzyme. Consistent with this view was the finding that physiological concentrations of fructose 1,6-bisphosphate inhibited in vitro phosphorylation of purified pyruvate kinase. Inactivation of pyruvate kinase by 0.3 nM glucagon or 1 microM phenylephrine was also suppressed by 10 nM insulin. Insulin did not act by increasing fructose 1,6-bisphosphate levels. The antagonism to glucagon correlated well with the ability of insulin to suppress activation of the cyclic AMP-dependent protein kinase. However, no such correlation was observed with phenylephrine in the absence or presence of insulin. Thus, insulin can enhance pyruvate kinase activity by both cyclic AMP-dependent and independent mechanisms.", "contents": "Modulation of the phosphorylation state of rat liver pyruvate kinase by allosteric effectors and insulin. The regulation of pyruvate kinase in isolated hepatocytes from fasted rats was studied where the intracellular level of fructose 1,6-bisphosphate was elevated 5-fold by the addition of 5 mM dihydroxyacetone. In this case, flux through pyruvate kinase was increased. The increase in flux correlated with an elevation in fructose bisphosphate levels but not with P-enolpyruvate levels which were unchanged. Pyruvate kinase was activated and its affinity for P-enolpyruvate was increased 7-fold in hepatocyte homogenates. Precipitation of the enzyme from homogenates with ammonium sulfate removed fructose 1,6-bisphosphate and activation was no longer observed. These results indicate that flux through and activity of pyruvate kinase can be controlled by the intracellular level of fructose 1,6-bisphosphate. The effect of elevated fructose 1,6-bisphosphate levels on the ability of glucagon to inactivate pyruvate kinase was also studied where only covalent enzyme modification is observed. Inactivation by maximally effective hormone concentrations was unaffected by elevated levels of fructose 1,6-bisphosphate, but the half-maximally effective concentration was increased from 0.3 to 0.8 nM. Activation of the cyclic AMP-dependent protein kinase by 0.3 nM glucagon was unaffected, but the initial rate of pyruvate kinase inactivation was suppressed. These results suggest that alterations in the level of fructose 1,6-bisphosphate can affect the ability of physiological concentrations of glucagon to inactivate pyruvate kinase by opposing phosphorylation of the enzyme. Consistent with this view was the finding that physiological concentrations of fructose 1,6-bisphosphate inhibited in vitro phosphorylation of purified pyruvate kinase. Inactivation of pyruvate kinase by 0.3 nM glucagon or 1 microM phenylephrine was also suppressed by 10 nM insulin. Insulin did not act by increasing fructose 1,6-bisphosphate levels. The antagonism to glucagon correlated well with the ability of insulin to suppress activation of the cyclic AMP-dependent protein kinase. However, no such correlation was observed with phenylephrine in the absence or presence of insulin. Thus, insulin can enhance pyruvate kinase activity by both cyclic AMP-dependent and independent mechanisms."}
{"id": "PMID:468794", "title": "Metabolism of arachidonic acid in polymorphonuclear leukocytes. Structural analysis of novel hydroxylated compounds.", "content": "Arachidonic acid was incubated with rabbit peritoneal polymorphonuclear leukocytes (glycogen-induced) and compounds obtained from ether extractions were fractionated by silicic acid column chromatography. A fraction containing several unidentified metabolites of arachidonic acid was analyzed by reversed phase-high pressure liquid chromatography. The metabolites were esterified and further purified by silicic acid high pressure liquid chromatography. The structures of the pure compounds were elucidated by infrared and ultraviolet spectrometry, ozonolysis, and gas chromatography-mass spectrometry. The following novel compounds were identified: Compound 1, 5S, 12R-dihydroxy-(E,E,E,Z)-6,8,10,14-eicosatetraenoic acid; Compound 2, 5S, 12S-dihydroxy-(E,E,E,Z)-6,8,10,14-eicosatetraenoic acid; Compound 3, 5, 6-dihydroxy-7,9,11,14-eicosatetraenoic acid; Compound 4, a diastereoisomer of the latter. Evidence for the occurrence of the delta-lactone forms of the 5,12-dihydroxy acids is also presented.", "contents": "Metabolism of arachidonic acid in polymorphonuclear leukocytes. Structural analysis of novel hydroxylated compounds. Arachidonic acid was incubated with rabbit peritoneal polymorphonuclear leukocytes (glycogen-induced) and compounds obtained from ether extractions were fractionated by silicic acid column chromatography. A fraction containing several unidentified metabolites of arachidonic acid was analyzed by reversed phase-high pressure liquid chromatography. The metabolites were esterified and further purified by silicic acid high pressure liquid chromatography. The structures of the pure compounds were elucidated by infrared and ultraviolet spectrometry, ozonolysis, and gas chromatography-mass spectrometry. The following novel compounds were identified: Compound 1, 5S, 12R-dihydroxy-(E,E,E,Z)-6,8,10,14-eicosatetraenoic acid; Compound 2, 5S, 12S-dihydroxy-(E,E,E,Z)-6,8,10,14-eicosatetraenoic acid; Compound 3, 5, 6-dihydroxy-7,9,11,14-eicosatetraenoic acid; Compound 4, a diastereoisomer of the latter. Evidence for the occurrence of the delta-lactone forms of the 5,12-dihydroxy acids is also presented."}
{"id": "PMID:468795", "title": "Determination of the ADP concentration available to participate in energy metabolism in an actin-rich cell, the platelet.", "content": "Almost all cells contain actin, which in its polymerized form, F-actin, binds 1 molecule of ADP/monomer. Little is known about the availability to metabolism of this bound ADP. A comparison was therefore made between perchloric acid and EDTA/ethanol extracts of human blood platelets. When the cells were extracted under conditions where the ATPase activity was negligible, the ethanol extracts had a 75% higher ATP/ADP ratio and a higher adenylate energy charge than perchloric acid extracts. The methods differed in that a considerable portion of protein-bound ADP was not extracted by ethanol. This bound ADP behaved as though it were unavailable to energy metabolism and should thus be considered as a compartment separate from the bulk metabolic pool of extragranular platelet adenine nucleotides. These results suggest that the level of ADP obtained with the common acid extraction overestimates the level available to participation in metabolism.", "contents": "Determination of the ADP concentration available to participate in energy metabolism in an actin-rich cell, the platelet. Almost all cells contain actin, which in its polymerized form, F-actin, binds 1 molecule of ADP/monomer. Little is known about the availability to metabolism of this bound ADP. A comparison was therefore made between perchloric acid and EDTA/ethanol extracts of human blood platelets. When the cells were extracted under conditions where the ATPase activity was negligible, the ethanol extracts had a 75% higher ATP/ADP ratio and a higher adenylate energy charge than perchloric acid extracts. The methods differed in that a considerable portion of protein-bound ADP was not extracted by ethanol. This bound ADP behaved as though it were unavailable to energy metabolism and should thus be considered as a compartment separate from the bulk metabolic pool of extragranular platelet adenine nucleotides. These results suggest that the level of ADP obtained with the common acid extraction overestimates the level available to participation in metabolism."}
{"id": "PMID:468799", "title": "Characterization of a somatomedin (insulin-like growth factor) synthesized by fetal rat liver organ cultures.", "content": "Explants of 19- to 20-day fetal rat liver synthesize polypeptides biochemically and immunologically related to the well characterized somatomedin (insulin-like growth factor) BRL-MSA, multiplication-stimulating activity. Fetal MSA was purified from media conditioned by fetal liver explants by chromatography on Sephadex G-75 under acid conditions. Partially purified fetal MSA: 1) inhibited the binding of BRL-MSA to the MSA receptor of rat liver plasma membranes, to somatomedin-binding proteins from rat serum, and to rabbit anti-BRL-MSA serum; 2) had a molecular weight of 4,500 to 12,500 determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate; 3) stimulated the incorporation of [3H]thymidine into the DNA of chick embryo fibroblasts and induced cell multiplication; 4) stimulated glucose oxidation in rat adipocytes and weakly inhibited the binding of insulin to the insulin receptors of IM-9 lymphocytes; and 5) stimulated sulfate uptake in costal cartilage from hypophysectomized rats. These activities were associated with the same molecular species in fetal MSA preparations following disc acrylamide electrophoresis and co-migrated with active BRL-MSA peptides.", "contents": "Characterization of a somatomedin (insulin-like growth factor) synthesized by fetal rat liver organ cultures. Explants of 19- to 20-day fetal rat liver synthesize polypeptides biochemically and immunologically related to the well characterized somatomedin (insulin-like growth factor) BRL-MSA, multiplication-stimulating activity. Fetal MSA was purified from media conditioned by fetal liver explants by chromatography on Sephadex G-75 under acid conditions. Partially purified fetal MSA: 1) inhibited the binding of BRL-MSA to the MSA receptor of rat liver plasma membranes, to somatomedin-binding proteins from rat serum, and to rabbit anti-BRL-MSA serum; 2) had a molecular weight of 4,500 to 12,500 determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate; 3) stimulated the incorporation of [3H]thymidine into the DNA of chick embryo fibroblasts and induced cell multiplication; 4) stimulated glucose oxidation in rat adipocytes and weakly inhibited the binding of insulin to the insulin receptors of IM-9 lymphocytes; and 5) stimulated sulfate uptake in costal cartilage from hypophysectomized rats. These activities were associated with the same molecular species in fetal MSA preparations following disc acrylamide electrophoresis and co-migrated with active BRL-MSA peptides."}
{"id": "PMID:468800", "title": "Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis.", "content": "Two dimensional gel electrophoresis has been used to measure the degree of genetic polymorphism among the proteins of normal human fibroblasts. Autoradiographic analysis of the gel protein profiles from radioactively labeled cells allowed comparison of as many as 300 discrete polypeptides at a time. In addition, a newly developed technique for double label autoradiography was used to increase the sensitivity of the system for detection of small differences in the protein profiles of different cell lines. Only about 1.2% of the proteins of different cell lines were found to differ in their electrophoretic mobility. This corresponds to an average heterozygosity of approximately 0.6%. Previous studies of genetic polymorphism using different methods of one-dimensional electrophoretic analysis have estimated the average heterozygosity of the human population at about 6.7%. Detailed mathematical analysis shows the variation of the observed from the expected number of differences to be statistically highly significant. While the reasons for this difference are not clear, the observation of low levels of genetic polymorphism on two-dimensional gels should enhance the usefulness of this technique for detection of altered proteins in inherited disease.", "contents": "Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis. Two dimensional gel electrophoresis has been used to measure the degree of genetic polymorphism among the proteins of normal human fibroblasts. Autoradiographic analysis of the gel protein profiles from radioactively labeled cells allowed comparison of as many as 300 discrete polypeptides at a time. In addition, a newly developed technique for double label autoradiography was used to increase the sensitivity of the system for detection of small differences in the protein profiles of different cell lines. Only about 1.2% of the proteins of different cell lines were found to differ in their electrophoretic mobility. This corresponds to an average heterozygosity of approximately 0.6%. Previous studies of genetic polymorphism using different methods of one-dimensional electrophoretic analysis have estimated the average heterozygosity of the human population at about 6.7%. Detailed mathematical analysis shows the variation of the observed from the expected number of differences to be statistically highly significant. While the reasons for this difference are not clear, the observation of low levels of genetic polymorphism on two-dimensional gels should enhance the usefulness of this technique for detection of altered proteins in inherited disease."}
{"id": "PMID:468801", "title": "Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines.", "content": "An improved method of two-dimensional gel electrophoresis has been developed which produces high resolution, reproducible images suitable for computer analysis. In the images that are presented, more than 800 proteins have been resolved without significant overlap, and many more proteins can be detected after longer exposures. To establish the usefulness of such methods for detailed quantitative comparisons of cultured cells, extensive controls have been carried out to test the reproducibility of the electrophoretic procedures, the sample preparation procedures, and the cell culture conditions. A computerized scanning system has been developed which can automatically detect and integrate the densities of the spots on a two-dimensional fluorogram or autoradiogram. The corresponding proteins from two or more samples can then be matched and their intensities compared. Several types of graphic output have been used to display the number and magnitude of the differences between the compared samples. These methods were used to study the patterns of protein synthesis in the nerve cell line B103 and the glial cell line B9. Both exponentially dividing and stationary cultures were analyzed and the relative rates of synthesis of approximately 300 proteins were compared by computer. For each cell line, no major qualitative differences were found between dividing and stationary phase cells although numerous quantitative differences of up to 15-fold were detected. The proteins that were increased or decreased in rate of synthesis as B103 cells became confluent were in general not the same proteins that were increased or decreased in rate of synthesis as B9 cells reached confluence, indicating that most of the changes do not reflect growth control responses common to all cells. When the two cell lines were analyzed in the same state of growth and compared by computer, qualitative differences were found in approximately 5% of the proteins analyzed, and at least 40% of the shared proteins were involved in quantitative differences of 2-fold or more. The rates of synthesis of the shared proteins were more divergent between the two cell lines than between dividing and stationary phase cells of either line. These studies show, therefore, that these cell lines can be distinguished, regardless of growth state, by their cell-specific proteins and by their characteristic rates of synthesis of many of the shared proteins.", "contents": "Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. An improved method of two-dimensional gel electrophoresis has been developed which produces high resolution, reproducible images suitable for computer analysis. In the images that are presented, more than 800 proteins have been resolved without significant overlap, and many more proteins can be detected after longer exposures. To establish the usefulness of such methods for detailed quantitative comparisons of cultured cells, extensive controls have been carried out to test the reproducibility of the electrophoretic procedures, the sample preparation procedures, and the cell culture conditions. A computerized scanning system has been developed which can automatically detect and integrate the densities of the spots on a two-dimensional fluorogram or autoradiogram. The corresponding proteins from two or more samples can then be matched and their intensities compared. Several types of graphic output have been used to display the number and magnitude of the differences between the compared samples. These methods were used to study the patterns of protein synthesis in the nerve cell line B103 and the glial cell line B9. Both exponentially dividing and stationary cultures were analyzed and the relative rates of synthesis of approximately 300 proteins were compared by computer. For each cell line, no major qualitative differences were found between dividing and stationary phase cells although numerous quantitative differences of up to 15-fold were detected. The proteins that were increased or decreased in rate of synthesis as B103 cells became confluent were in general not the same proteins that were increased or decreased in rate of synthesis as B9 cells reached confluence, indicating that most of the changes do not reflect growth control responses common to all cells. When the two cell lines were analyzed in the same state of growth and compared by computer, qualitative differences were found in approximately 5% of the proteins analyzed, and at least 40% of the shared proteins were involved in quantitative differences of 2-fold or more. The rates of synthesis of the shared proteins were more divergent between the two cell lines than between dividing and stationary phase cells of either line. These studies show, therefore, that these cell lines can be distinguished, regardless of growth state, by their cell-specific proteins and by their characteristic rates of synthesis of many of the shared proteins."}
{"id": "PMID:468802", "title": "Purification of RNA-DNA hybrids by exclusion chromatography.", "content": "A simple method for selection of RNA-DNA hybrids has been developed and applied to the purification of adenovirus-specific messenger RNA. Cytoplasmic RNA prepared from adenovirus type 2 (ad2)-infected HeLa cells or from an ad2-transformed rat cell line was hybridized in solution to the complementary strands of ad2 DNA. The hybridization mixture was subsequently fractionated by chromatography on a Sepharose 2B column. The intact probe DNA as well as the RNA-DNA hybrids are excluded from the gel matrix and elute with the void volume. Nonhybridized RNA, in contrast, is included into the gel matrix and elutes as a broad peak well separated from the excluded fractions. Fractions corresponding to the void volume, were collected and the RNA-DNA hybrids were denatured in 90% formamide. The selected RNA was separated from the DNA by affinity chromatography on poly(U)-Sepharose. Restriction endonuclease fragments of DNA with a large enough size to make them excluded from the agarose column were also used for hybridization. In these experiments hybridizations were carried out under conditions which would allow R-loop formation (Thomas, M., White, R.L., and Davis, R.W. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 2294-2298) and the hybridized RNA was separated from unhybridized RNA by Sepharose chromatography. The validity of the method was demonstrated by programming an in vitro protein-synthesizing system with selected RNA.", "contents": "Purification of RNA-DNA hybrids by exclusion chromatography. A simple method for selection of RNA-DNA hybrids has been developed and applied to the purification of adenovirus-specific messenger RNA. Cytoplasmic RNA prepared from adenovirus type 2 (ad2)-infected HeLa cells or from an ad2-transformed rat cell line was hybridized in solution to the complementary strands of ad2 DNA. The hybridization mixture was subsequently fractionated by chromatography on a Sepharose 2B column. The intact probe DNA as well as the RNA-DNA hybrids are excluded from the gel matrix and elute with the void volume. Nonhybridized RNA, in contrast, is included into the gel matrix and elutes as a broad peak well separated from the excluded fractions. Fractions corresponding to the void volume, were collected and the RNA-DNA hybrids were denatured in 90% formamide. The selected RNA was separated from the DNA by affinity chromatography on poly(U)-Sepharose. Restriction endonuclease fragments of DNA with a large enough size to make them excluded from the agarose column were also used for hybridization. In these experiments hybridizations were carried out under conditions which would allow R-loop formation (Thomas, M., White, R.L., and Davis, R.W. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 2294-2298) and the hybridized RNA was separated from unhybridized RNA by Sepharose chromatography. The validity of the method was demonstrated by programming an in vitro protein-synthesizing system with selected RNA."}
{"id": "PMID:468803", "title": "Differential dependence of reovirus-associated enzyme activities on genome RNA as determined by psoralen photosensitivity.", "content": "Irradiation with long wavelength UV light in the presence of 4'-substituted psoralens abolished the in fectivity of reovirus and reovirus subviral particles Photoreaction of intact virions, cores, or genome RNA resulted in the formation of psoralen monoadducts and cross-links, demonstrating that the viral genome RNA in situ is double-stranded. Virus-associated RNA polymerase activity was lost following psoralen photoreaction, consistent with dependence on the genome RNA as template for viral mRNA synthesis. By contrast, reovirus guanylyl transferase and methyl transferase activities that catalyze the formation of mRNA 5'-terminal cap, m GpppG, were relatively unaffected by photoreaction. Cations that inhibit psoralen binding to nucleic acids also protected against loss of RNA polymerase activity. Partially photoinactivated viral cores produced decreased amounts of full length viral mRNAs. The absence of prematurely terminated transcripts indicates that template RNA modification by psoralen adducts has an all-or-none effect on initiation of mRNA synthesis by the core-associated RNA polymerase.", "contents": "Differential dependence of reovirus-associated enzyme activities on genome RNA as determined by psoralen photosensitivity. Irradiation with long wavelength UV light in the presence of 4'-substituted psoralens abolished the in fectivity of reovirus and reovirus subviral particles Photoreaction of intact virions, cores, or genome RNA resulted in the formation of psoralen monoadducts and cross-links, demonstrating that the viral genome RNA in situ is double-stranded. Virus-associated RNA polymerase activity was lost following psoralen photoreaction, consistent with dependence on the genome RNA as template for viral mRNA synthesis. By contrast, reovirus guanylyl transferase and methyl transferase activities that catalyze the formation of mRNA 5'-terminal cap, m GpppG, were relatively unaffected by photoreaction. Cations that inhibit psoralen binding to nucleic acids also protected against loss of RNA polymerase activity. Partially photoinactivated viral cores produced decreased amounts of full length viral mRNAs. The absence of prematurely terminated transcripts indicates that template RNA modification by psoralen adducts has an all-or-none effect on initiation of mRNA synthesis by the core-associated RNA polymerase."}
{"id": "PMID:468806", "title": "Radioiodination of chicken erythrocyte histones H4 and H5 in chromatin.", "content": "The conformational state of histones in isolated chicken erythrocyte chromatin was studied using procedures developed for probing surface proteins on membranes. Under controlled conditions, only exposed tyrosyl residues react with iodide radicals, generated either by the oxidant, chloramine-T (paratoluenesulfonyl chloramide), or the enzyme lactoperoxidase, giving monoidotyrosine. Using 125-iodine, this study compared the reactive tyrosines in free and bound histones H4, and H5. The relative extent of iodination of these histones within (H4) and outside (H5) of the nucleosomes was measured after extraction and gel electrophoresis. Each of the histones was further analyzed for the extent of specific tyrosine iodination by separating the tryptic peptides by high voltage electrophoresis. The identity of the labeled peptide was determined by dansylation of the amino acids present in each hydrolyzed peptide. The results show that there is a difference in the conformational arrangement of these histones on chromatin and in the free forms, since in chromatin not all tyrosine residues are as accessible for iodination as in the denatured state. Residue 53 of histone H5 for instance is more reactive than residues 28 and 58, indicating that the segments containing the latter residues are involved in either protein-DNA or protein-protein interactions. In histone H4, preferential labeling of 2 of the 4 tyrosines present was also observed.", "contents": "Radioiodination of chicken erythrocyte histones H4 and H5 in chromatin. The conformational state of histones in isolated chicken erythrocyte chromatin was studied using procedures developed for probing surface proteins on membranes. Under controlled conditions, only exposed tyrosyl residues react with iodide radicals, generated either by the oxidant, chloramine-T (paratoluenesulfonyl chloramide), or the enzyme lactoperoxidase, giving monoidotyrosine. Using 125-iodine, this study compared the reactive tyrosines in free and bound histones H4, and H5. The relative extent of iodination of these histones within (H4) and outside (H5) of the nucleosomes was measured after extraction and gel electrophoresis. Each of the histones was further analyzed for the extent of specific tyrosine iodination by separating the tryptic peptides by high voltage electrophoresis. The identity of the labeled peptide was determined by dansylation of the amino acids present in each hydrolyzed peptide. The results show that there is a difference in the conformational arrangement of these histones on chromatin and in the free forms, since in chromatin not all tyrosine residues are as accessible for iodination as in the denatured state. Residue 53 of histone H5 for instance is more reactive than residues 28 and 58, indicating that the segments containing the latter residues are involved in either protein-DNA or protein-protein interactions. In histone H4, preferential labeling of 2 of the 4 tyrosines present was also observed."}
{"id": "PMID:468807", "title": "The isolation and amino acid/sugar composition of human fibroblastoid interferon.", "content": "Human fibroblastoid interferon produced from an established human cell line was purified by controlled-pore glass and concanavalin A-Sepharose column chromatography followed by preparative two-dimensional gel electrophoresis. The purification procedure provided a 10% recovery of pure interferon with good reproducibility. The purified protein was homogeneous with respect to its molecular weight of 20,000 and net electrical charge at pH 2.5. Interferon of high specific activity of 5 x 10(8) units/mg of protein was directly demonstrated in the polyacrylamide gel before staining with Coomassie brilliant blue. Parallel purification of a sham-induced interferon preparation did not yield an equivalent product indicating the purified interferon is not derived from uninduced cells or from the fetal calf serum of the tissue culture growth medium. Pure interferon was radioiodinated by Bolton-Hunter reagent. Amino acid analysis of the pure preparation shows interferon to be a leucine-rich glycoprotein containing a high percentage of glutamic/glutamine residues and that disulfide bridges(s) are important for its biological activity.", "contents": "The isolation and amino acid/sugar composition of human fibroblastoid interferon. Human fibroblastoid interferon produced from an established human cell line was purified by controlled-pore glass and concanavalin A-Sepharose column chromatography followed by preparative two-dimensional gel electrophoresis. The purification procedure provided a 10% recovery of pure interferon with good reproducibility. The purified protein was homogeneous with respect to its molecular weight of 20,000 and net electrical charge at pH 2.5. Interferon of high specific activity of 5 x 10(8) units/mg of protein was directly demonstrated in the polyacrylamide gel before staining with Coomassie brilliant blue. Parallel purification of a sham-induced interferon preparation did not yield an equivalent product indicating the purified interferon is not derived from uninduced cells or from the fetal calf serum of the tissue culture growth medium. Pure interferon was radioiodinated by Bolton-Hunter reagent. Amino acid analysis of the pure preparation shows interferon to be a leucine-rich glycoprotein containing a high percentage of glutamic/glutamine residues and that disulfide bridges(s) are important for its biological activity."}
{"id": "PMID:468808", "title": "Nonidentity of the 48,000-dalton protein of mRNA-protein particles and the beta subunit of eukaryotic initiation factor 2.", "content": "The beta subunits of the GTP-dependent initiator methionyl-tRNA-binding protein and the 48,000-dalton protein, which is one of the two major proteins present in isolated mRNA . protein particles, have been shown to share a number of similar structural and mRNA-binding properties. The nonidentity of these two proteins is now established by documentation of differing molecular weights, isoelectric focusing points, and products of protease or cyanogen bromide cleavage.", "contents": "Nonidentity of the 48,000-dalton protein of mRNA-protein particles and the beta subunit of eukaryotic initiation factor 2. The beta subunits of the GTP-dependent initiator methionyl-tRNA-binding protein and the 48,000-dalton protein, which is one of the two major proteins present in isolated mRNA . protein particles, have been shown to share a number of similar structural and mRNA-binding properties. The nonidentity of these two proteins is now established by documentation of differing molecular weights, isoelectric focusing points, and products of protease or cyanogen bromide cleavage."}
{"id": "PMID:468810", "title": "An alternating conformation characterizes the phosphodiester backbone of poly(dA-dT) in solution.", "content": "A highly homogeneous 145-base-pair fragment of double helical poly(dA-dT) . poly(dA-dT) was obtained by micrococcal nuclease digestion of a semisynthetic chromatin prepared from the nucleosome core histones (H2A, H2B, H3, H4) and the synthetic polydeoxyribonucleotide. In contrast to higher molecular weight alternating copolymers, this fragment displayed two resolved 31P NMR signals, separated by 24 Hz at 10.93 MHz. The two signals were of equal intensity at all temperatures less than the Tm for the fragment. Analyses of the possible origins for the two reasonances leads to the conclusion that the phosphodiester backbone of this DNA contains two distinct phosphorus environments, probably in an alternating array. We suggest that this may indicate the presence of sequence-dependent local variation in the helical structure of DNA in general.", "contents": "An alternating conformation characterizes the phosphodiester backbone of poly(dA-dT) in solution. A highly homogeneous 145-base-pair fragment of double helical poly(dA-dT) . poly(dA-dT) was obtained by micrococcal nuclease digestion of a semisynthetic chromatin prepared from the nucleosome core histones (H2A, H2B, H3, H4) and the synthetic polydeoxyribonucleotide. In contrast to higher molecular weight alternating copolymers, this fragment displayed two resolved 31P NMR signals, separated by 24 Hz at 10.93 MHz. The two signals were of equal intensity at all temperatures less than the Tm for the fragment. Analyses of the possible origins for the two reasonances leads to the conclusion that the phosphodiester backbone of this DNA contains two distinct phosphorus environments, probably in an alternating array. We suggest that this may indicate the presence of sequence-dependent local variation in the helical structure of DNA in general."}
{"id": "PMID:468811", "title": "Active transport of thallous ions by Streptococcus lactis.", "content": "Tl+ ions have been shown to mimic or compete with K+ in a number of membrane systems. We confirmed that in starved, valinomycin-treated cells of Streptococcus lactis 7962, Tl+ ions distributed themselves across the bacterial membrane in response to the potassium diffusion potential. In glucose-energized cells, however, Tl+ was taken up by a system specifically stimulated by sodium salts. The intracellular levels of Tl+ exceeded those attained by [3H]triphenylmethylphosphonium ion, a lipophilic cation which accumulates in response to the membrane potential. The uptake of Tl+ by (Na+ and glucose)-stimulated cells was strongly inhibited by potassium salts. These experiments suggest that metabolic energy is coupled to Tl+ transport by means of a high energy phosphate compound and that Tl+ ions are actively transported by a membrane carrier whose normal substrate is K+. The uptake of Tl+ is not a valid method for determining the streptococcal membrane potential.", "contents": "Active transport of thallous ions by Streptococcus lactis. Tl+ ions have been shown to mimic or compete with K+ in a number of membrane systems. We confirmed that in starved, valinomycin-treated cells of Streptococcus lactis 7962, Tl+ ions distributed themselves across the bacterial membrane in response to the potassium diffusion potential. In glucose-energized cells, however, Tl+ was taken up by a system specifically stimulated by sodium salts. The intracellular levels of Tl+ exceeded those attained by [3H]triphenylmethylphosphonium ion, a lipophilic cation which accumulates in response to the membrane potential. The uptake of Tl+ by (Na+ and glucose)-stimulated cells was strongly inhibited by potassium salts. These experiments suggest that metabolic energy is coupled to Tl+ transport by means of a high energy phosphate compound and that Tl+ ions are actively transported by a membrane carrier whose normal substrate is K+. The uptake of Tl+ is not a valid method for determining the streptococcal membrane potential."}
{"id": "PMID:468812", "title": "Similarity of the structure of ferritin and iron . dextran (imferon) determined by extended X-ray absorption fine structure analysis.", "content": "Ferritin, a natural complex of iron oxide encased in protein, and iron . dextran, a synthetic complex of iron oxide coated with dextran, have the similar properties of maintaining high concentrations of iron in solution at physiological pH and releasing iron relatively slowly in vivo. Extended x-ray absorption fine structure (EX-AFS) analysis was performed on each complex and compared to see if the structures of the iron cores were similar. The results obtained from the extended x-ray absorption fine structure technique show that the near-neighbor environment around the average iron atom in ferritin and iron . dextran is identical, within experimental uncertainty, for the first three shells. The similarity of the iron cores in both complexes may explain the similarity of iron release in vivo. Ferritin has a protein coat which is composed of 24 subunits arranged in a hollow sphere with six channels through which the iron may move during deposition and release. However, little is known about the requirements of the protein structure in ferritin for the maintenance of high concentrations of iron in a soluble, nontoxic form or about the role of the protein in the release of iron from ferritin. The results suggest that iron . dextran will be a useful model compound in studies of the relation of the iron core and protein in ferritin to function.", "contents": "Similarity of the structure of ferritin and iron . dextran (imferon) determined by extended X-ray absorption fine structure analysis. Ferritin, a natural complex of iron oxide encased in protein, and iron . dextran, a synthetic complex of iron oxide coated with dextran, have the similar properties of maintaining high concentrations of iron in solution at physiological pH and releasing iron relatively slowly in vivo. Extended x-ray absorption fine structure (EX-AFS) analysis was performed on each complex and compared to see if the structures of the iron cores were similar. The results obtained from the extended x-ray absorption fine structure technique show that the near-neighbor environment around the average iron atom in ferritin and iron . dextran is identical, within experimental uncertainty, for the first three shells. The similarity of the iron cores in both complexes may explain the similarity of iron release in vivo. Ferritin has a protein coat which is composed of 24 subunits arranged in a hollow sphere with six channels through which the iron may move during deposition and release. However, little is known about the requirements of the protein structure in ferritin for the maintenance of high concentrations of iron in a soluble, nontoxic form or about the role of the protein in the release of iron from ferritin. The results suggest that iron . dextran will be a useful model compound in studies of the relation of the iron core and protein in ferritin to function."}
{"id": "PMID:468813", "title": "A high molecular weight protease in liver cytosol.", "content": "A high molecular weight (greater than 400,000) protease active with [3H]leucine-labeled globin has been found in the postmicrosomal fraction of mouse kidney, brain, heart, spleen, and tumor cells and is most active in liver. The presence in liver was unexpected because liver cytosol is very ineffective in the breakdown of endogenous, labeled proteins. The enzyme has a number of properties that distinguish it from known cathepsins in addition to its high molecular weight. It is most active at pH approximately 7.5. When purified, it is unstable above 20 degrees C and is stabilized by metal chelating agents such as citrate, creatine-P, and glycerate-3-P. It is an -SH protease, but its thermal instability is not affected by 1 mM dithiothreitol. The enzyme is not lysosomal.", "contents": "A high molecular weight protease in liver cytosol. A high molecular weight (greater than 400,000) protease active with [3H]leucine-labeled globin has been found in the postmicrosomal fraction of mouse kidney, brain, heart, spleen, and tumor cells and is most active in liver. The presence in liver was unexpected because liver cytosol is very ineffective in the breakdown of endogenous, labeled proteins. The enzyme has a number of properties that distinguish it from known cathepsins in addition to its high molecular weight. It is most active at pH approximately 7.5. When purified, it is unstable above 20 degrees C and is stabilized by metal chelating agents such as citrate, creatine-P, and glycerate-3-P. It is an -SH protease, but its thermal instability is not affected by 1 mM dithiothreitol. The enzyme is not lysosomal."}
{"id": "PMID:468814", "title": "One-electron reduction of flavodoxin. A fast kinetic study.", "content": "The reaction kinetics of fully oxidized flavodoxin from Clostridium MP with the hydrated electron have been investigated by the pulse radiolysis method. Four spectrally distinct processes have been observed with the ultimate formation of the singly reduced flavin form of the protein. The last two species obtained in the reaction sequence are spectrally similar, and are connected through a reaction which is first order. It is proposed that this reaction involves a protein conformational alteration.", "contents": "One-electron reduction of flavodoxin. A fast kinetic study. The reaction kinetics of fully oxidized flavodoxin from Clostridium MP with the hydrated electron have been investigated by the pulse radiolysis method. Four spectrally distinct processes have been observed with the ultimate formation of the singly reduced flavin form of the protein. The last two species obtained in the reaction sequence are spectrally similar, and are connected through a reaction which is first order. It is proposed that this reaction involves a protein conformational alteration."}
{"id": "PMID:468815", "title": "Enoyl coenzyme A reduction by bovine mammary fatty acid synthetase. Specificity and other characteristics.", "content": "The NADPH-dependent enoyl coenzyme A reductase activity of bovine mammary fatty acid synthetase has been characterized with regard to substrate specificity and the product formed. A relatively high specificity for an unsubstituted, four-carbon, 2,3-enoyl chain in trans configuration is obtained. Reduction of trans-crotonyl-CoA results in butyrate, 50% of which is coenzyme A-bound. The reaction is subject to product inhibition, specifically by butyryl-CoA and NADP. Free coenzyme A, on the other hand, is an activator. The pH profile, susceptibility to inhibition by -SH reagents, the results of the relative activities obtained with substrate analogues and homologues, and the ready use of crotonyl-CoA as a primer in fatty acid synthesis are consistent with a mechanism in which the crotonyl group is transferred to an -SH group, is reduced, and then is either transferred back to CoA or hydrolyzed.", "contents": "Enoyl coenzyme A reduction by bovine mammary fatty acid synthetase. Specificity and other characteristics. The NADPH-dependent enoyl coenzyme A reductase activity of bovine mammary fatty acid synthetase has been characterized with regard to substrate specificity and the product formed. A relatively high specificity for an unsubstituted, four-carbon, 2,3-enoyl chain in trans configuration is obtained. Reduction of trans-crotonyl-CoA results in butyrate, 50% of which is coenzyme A-bound. The reaction is subject to product inhibition, specifically by butyryl-CoA and NADP. Free coenzyme A, on the other hand, is an activator. The pH profile, susceptibility to inhibition by -SH reagents, the results of the relative activities obtained with substrate analogues and homologues, and the ready use of crotonyl-CoA as a primer in fatty acid synthesis are consistent with a mechanism in which the crotonyl group is transferred to an -SH group, is reduced, and then is either transferred back to CoA or hydrolyzed."}
{"id": "PMID:468817", "title": "alpha Chain mutations with opposite effects on the gelation of hemoglobin S.", "content": "The preparation of three hemoglobin tetramers containing the hemoglobin S mutation at beta 6 and an additional one at alpha 6, alpha 47, and alpha 75 is described. The effect of the substitutions in the alpha chains on polymerization was investigated by the equilibrium solubility of the gels as well as the abrupt change in oxygen affinity associated with the onset of gelation. Substitution of a histidine for aspartic acid at alpha 47 causes a marked inhibition of polymerization. This inhibition probably results from tetramers which carry the two substitutions on the same alpha beta dimer. By contrast, the introduction of a tyrosine at alpha 75 and an alanine at alpha 6 have the opposite effect and are the first examples of alpha chain mutations which potentiate the gelation of Hb S. The molecular mechanisms responsible for the effects of the mutations on the self-association of Hb S are discussed.", "contents": "alpha Chain mutations with opposite effects on the gelation of hemoglobin S. The preparation of three hemoglobin tetramers containing the hemoglobin S mutation at beta 6 and an additional one at alpha 6, alpha 47, and alpha 75 is described. The effect of the substitutions in the alpha chains on polymerization was investigated by the equilibrium solubility of the gels as well as the abrupt change in oxygen affinity associated with the onset of gelation. Substitution of a histidine for aspartic acid at alpha 47 causes a marked inhibition of polymerization. This inhibition probably results from tetramers which carry the two substitutions on the same alpha beta dimer. By contrast, the introduction of a tyrosine at alpha 75 and an alanine at alpha 6 have the opposite effect and are the first examples of alpha chain mutations which potentiate the gelation of Hb S. The molecular mechanisms responsible for the effects of the mutations on the self-association of Hb S are discussed."}
{"id": "PMID:468822", "title": "Isolation and characterization of gangliosides with a new sialosyl linkage and core structures. II. Gangliosides of human erythrocyte membranes.", "content": "Eight monosialosylgangliosides, G1 to G8, have been isolated from human erythrocyte membranes and their structures have been determined. Gangliosides G4 and G7 have been characterized by having 2 leads to 6-linked sialic acid to galactose at their termini. Ganglioside G5 was a positional isomer of a brain ganglioside GM1 as to the linkage of sialic acid. Ganglioside G8 was characterized as a branched chain ganglioside similar to a fucoganglioside previously isolated but devoid of fucose, and it showed a strong blood group I activity. Structures of these four new gangliosides are shown below: (formula: see text).", "contents": "Isolation and characterization of gangliosides with a new sialosyl linkage and core structures. II. Gangliosides of human erythrocyte membranes. Eight monosialosylgangliosides, G1 to G8, have been isolated from human erythrocyte membranes and their structures have been determined. Gangliosides G4 and G7 have been characterized by having 2 leads to 6-linked sialic acid to galactose at their termini. Ganglioside G5 was a positional isomer of a brain ganglioside GM1 as to the linkage of sialic acid. Ganglioside G8 was characterized as a branched chain ganglioside similar to a fucoganglioside previously isolated but devoid of fucose, and it showed a strong blood group I activity. Structures of these four new gangliosides are shown below: (formula: see text)."}
{"id": "PMID:468825", "title": "Threshold effects on the concanavalin A-mediated agglutination of modified erythrocytes.", "content": "Bovine erythrocytes, which are not concanavalin A (ConA)-agglutinable, can be rendered so by attaching alpha-D-mannose residues to their outer membrane. The sugars are incorporated by mildly oxidizing the cells with periodate followed by coupling the liberated aldehyde groups with an alpha-thiomannosyl containing hydrazide (I). The rate and extent of ConA-mediated aggregation of the modified cells are not linearly dependent on the amount of sugar incorporated. For example, treatment of the erythrocytes with 0.075 mM periodate for 5 min followed by I led to the introduction of 1.05 x 10(6) mannosyl residues/erythrocyte. Binding studies with 125I-ConA demonstrated the presence of 66,525 ConA receptors/cell with an average KA = 4.9 X 10(6) M-1 yet the cells failed to aggregate with ConA at concentrations up to 500 microgram ml-1. Treating the cells with 0.1 mM periodate followed by I led to the introduction of 1.42 x 10(6) mannosyl residues/erythrocyte. Binding studies with 125I-ConA indicated the presence of 78,780 binding sites/cell (KA = 5.9 X 10(6) M-1). These cells were readily aggregated by ConA at concentrations greater than or equal to 64 microgram ml-1. We show here that the sugar incorporation technique is random and that no functional differences were detected in the receptors introduced at the different periodate concentrations. Therefore, the ConA-mediated aggregation of these modified erythrocytes is exquisitely sensitive to small changes in functionally identical receptor densities.", "contents": "Threshold effects on the concanavalin A-mediated agglutination of modified erythrocytes. Bovine erythrocytes, which are not concanavalin A (ConA)-agglutinable, can be rendered so by attaching alpha-D-mannose residues to their outer membrane. The sugars are incorporated by mildly oxidizing the cells with periodate followed by coupling the liberated aldehyde groups with an alpha-thiomannosyl containing hydrazide (I). The rate and extent of ConA-mediated aggregation of the modified cells are not linearly dependent on the amount of sugar incorporated. For example, treatment of the erythrocytes with 0.075 mM periodate for 5 min followed by I led to the introduction of 1.05 x 10(6) mannosyl residues/erythrocyte. Binding studies with 125I-ConA demonstrated the presence of 66,525 ConA receptors/cell with an average KA = 4.9 X 10(6) M-1 yet the cells failed to aggregate with ConA at concentrations up to 500 microgram ml-1. Treating the cells with 0.1 mM periodate followed by I led to the introduction of 1.42 x 10(6) mannosyl residues/erythrocyte. Binding studies with 125I-ConA indicated the presence of 78,780 binding sites/cell (KA = 5.9 X 10(6) M-1). These cells were readily aggregated by ConA at concentrations greater than or equal to 64 microgram ml-1. We show here that the sugar incorporation technique is random and that no functional differences were detected in the receptors introduced at the different periodate concentrations. Therefore, the ConA-mediated aggregation of these modified erythrocytes is exquisitely sensitive to small changes in functionally identical receptor densities."}
{"id": "PMID:468827", "title": "Partial and incomplete oxidation of palmitate by cultured beating cardiac cells from neonatal rats.", "content": "The discrepancy in the rate of [14C]O2 formation from either [1-14C]- or [16-14C]palmitate is demonstrated and could be explained by the preferential formation of L-(+)-3-hydroxybutyrate from the four carbon atoms at the omega terminus. The identity of this product as L(+)-3-hydroxybutyrate was established and shown to be the major component of the radioactive products in the extracellular medium from palmitate based on (a) ion-exchange chromatographical properties, (b) gas-liquid chromatography, (c) mass spectrometric analysis, (d) stereoisomeric separation, and (e) its very low rate of utilization by the cells. We therefore propose a shunt to the oxidation of palmitate in these cells occurring at the stage of L(+)-hydroxybutyryl-CoA which undergoes deacylation causing the product to be transported outside the cell.", "contents": "Partial and incomplete oxidation of palmitate by cultured beating cardiac cells from neonatal rats. The discrepancy in the rate of [14C]O2 formation from either [1-14C]- or [16-14C]palmitate is demonstrated and could be explained by the preferential formation of L-(+)-3-hydroxybutyrate from the four carbon atoms at the omega terminus. The identity of this product as L(+)-3-hydroxybutyrate was established and shown to be the major component of the radioactive products in the extracellular medium from palmitate based on (a) ion-exchange chromatographical properties, (b) gas-liquid chromatography, (c) mass spectrometric analysis, (d) stereoisomeric separation, and (e) its very low rate of utilization by the cells. We therefore propose a shunt to the oxidation of palmitate in these cells occurring at the stage of L(+)-hydroxybutyryl-CoA which undergoes deacylation causing the product to be transported outside the cell."}
{"id": "PMID:468828", "title": "Resonance Raman scattering from uteroferrin, the purple glycoprotein of the porcine uterus.", "content": "Uteroferrin, a purple-colored, iron-containing glycoprotein, purified from the uterine fluid of progesterone-stimulated pigs, owes its natural purple color to a broad absorption band centered at 545 nm. Laser excitation within the visible absorption band of uteroferrin results in an intense resonance Raman spectrum which bears a striking resemblance to that reported for Fe(III)-transferrin, the iron transport protein of serum. Excitation profiles for the four resonance-enhanced bands of uteroferrin were obtained from 4579 A to 6741 A, using lines from Ar+ and Kr+ lasers. Each of the profiles have maxima near 545 nm. The spectral similarities of uteroferrin and Fe(III)-transferrin lead to the belief that the Fe(III) binding sites of the two proteins must be, at least in some respects, quite similar. In particular, it is concluded that, as in Fe(III)-transferrin, the metal binding site of uteroferrin contains a tyrosine ligand and that the visible absorption spectrum of uteroferrin results from a phenolate to Fe(III) charge transfer.", "contents": "Resonance Raman scattering from uteroferrin, the purple glycoprotein of the porcine uterus. Uteroferrin, a purple-colored, iron-containing glycoprotein, purified from the uterine fluid of progesterone-stimulated pigs, owes its natural purple color to a broad absorption band centered at 545 nm. Laser excitation within the visible absorption band of uteroferrin results in an intense resonance Raman spectrum which bears a striking resemblance to that reported for Fe(III)-transferrin, the iron transport protein of serum. Excitation profiles for the four resonance-enhanced bands of uteroferrin were obtained from 4579 A to 6741 A, using lines from Ar+ and Kr+ lasers. Each of the profiles have maxima near 545 nm. The spectral similarities of uteroferrin and Fe(III)-transferrin lead to the belief that the Fe(III) binding sites of the two proteins must be, at least in some respects, quite similar. In particular, it is concluded that, as in Fe(III)-transferrin, the metal binding site of uteroferrin contains a tyrosine ligand and that the visible absorption spectrum of uteroferrin results from a phenolate to Fe(III) charge transfer."}
{"id": "PMID:468829", "title": "Mechanism of phosphoacetylglucosamine mutase.", "content": "Kinetic studies of phosphoacetylglucosamine mutase (EC 2.7.5.2) for the following reactions: 1) Glc-1-P in equilibrium Glc-6-P and 2) GlcNAc-1-P in equilibrium GlcNAc-6-P have been conducted in the presence of Glc-1,6-P2 and GlcNAc-1,6-P2, respectively. In the first reaction, the initial velocity studies at various concentrations of one substrate showed a series of parallel lines in the Line-weaver-Burk plot when the concentrations of the other substrate were changed at several fixed levels. For both reactions, the initial velocity studies performed at fixed ratios of both substrates showed linear lines in the double reciprocal plot. The competitive substrate inhibition pattern was observed in the second reaction. A ping-pong mechanism is proposed for phosphoacetyl-glucosamine mutase. In addition, phosphoacetylglucosamine mutase can be phosphorylated by the addition of Glc-1-[32P]P probably via the reaction of Glc-1-[32P]P with the phosphoenzyme followed by the release of glucose-monophosphate leaving the 32P with the phosphoenzyme. The linkage between the phosphoryl residue and enzyme is stable in acid, but labile in alkali, suggesting phosphoserine (or phosphothreonine) as the phosphorylated amino acid. Biphasic heat denaturation curves suggest the existence of heat-stable and heat-labile forms of this enzyme.", "contents": "Mechanism of phosphoacetylglucosamine mutase. Kinetic studies of phosphoacetylglucosamine mutase (EC 2.7.5.2) for the following reactions: 1) Glc-1-P in equilibrium Glc-6-P and 2) GlcNAc-1-P in equilibrium GlcNAc-6-P have been conducted in the presence of Glc-1,6-P2 and GlcNAc-1,6-P2, respectively. In the first reaction, the initial velocity studies at various concentrations of one substrate showed a series of parallel lines in the Line-weaver-Burk plot when the concentrations of the other substrate were changed at several fixed levels. For both reactions, the initial velocity studies performed at fixed ratios of both substrates showed linear lines in the double reciprocal plot. The competitive substrate inhibition pattern was observed in the second reaction. A ping-pong mechanism is proposed for phosphoacetyl-glucosamine mutase. In addition, phosphoacetylglucosamine mutase can be phosphorylated by the addition of Glc-1-[32P]P probably via the reaction of Glc-1-[32P]P with the phosphoenzyme followed by the release of glucose-monophosphate leaving the 32P with the phosphoenzyme. The linkage between the phosphoryl residue and enzyme is stable in acid, but labile in alkali, suggesting phosphoserine (or phosphothreonine) as the phosphorylated amino acid. Biphasic heat denaturation curves suggest the existence of heat-stable and heat-labile forms of this enzyme."}
{"id": "PMID:468830", "title": "Effect of leucine and metabolites of branched chain amino acids on protein turnover in heart.", "content": "Leucine, but not isoleucine or valine, inhibited protein degradation and accelerated protein synthesis in hearts perfused with buffer that contained glucose (15 mM) and normal plasma levels of other amino acids, except for the branched chain compounds. Products of leucine, isoleucine, and valine metabolism also inhibited protein degradation and stimulated protein synthesis. These compounds included the transamination and decarboxylation products, as well as acetate, acetoacetate, and propionate. In some, but not all instances, inhibition of degradation and acceleration of synthesis were accompanied by an increase in intracellular leucine. When insulin was added to the perfusate, the rate of degradation was reduced by 40%, but addition of leucine was ineffective in the presence of the hormone. Insulin, leucine (2 mM) and a mixture of branched chain amino acids at normal plasma levels increased latency of cathepsin D in hearts that were perfused with buffer containing glucose. A combination of leucine and insulin increased latency more than either substance alone. These studies indicate that leucine as well as a variety of substrates that are oxidized in the citric acid cycle are involved in regulation of protein turnover in heart muscle.", "contents": "Effect of leucine and metabolites of branched chain amino acids on protein turnover in heart. Leucine, but not isoleucine or valine, inhibited protein degradation and accelerated protein synthesis in hearts perfused with buffer that contained glucose (15 mM) and normal plasma levels of other amino acids, except for the branched chain compounds. Products of leucine, isoleucine, and valine metabolism also inhibited protein degradation and stimulated protein synthesis. These compounds included the transamination and decarboxylation products, as well as acetate, acetoacetate, and propionate. In some, but not all instances, inhibition of degradation and acceleration of synthesis were accompanied by an increase in intracellular leucine. When insulin was added to the perfusate, the rate of degradation was reduced by 40%, but addition of leucine was ineffective in the presence of the hormone. Insulin, leucine (2 mM) and a mixture of branched chain amino acids at normal plasma levels increased latency of cathepsin D in hearts that were perfused with buffer containing glucose. A combination of leucine and insulin increased latency more than either substance alone. These studies indicate that leucine as well as a variety of substrates that are oxidized in the citric acid cycle are involved in regulation of protein turnover in heart muscle."}
{"id": "PMID:468839", "title": "The covalent differences between bovine alpha- and beta-thrombin. A structural explanation for the changes in catalytic activity.", "content": "The partial covalent structure of bovine beta-thrombin has been determined by the use of automated Edman degradation and carboxypeptidase digestion of the component polypeptide chains separated by gel filtration following either reduction and carboxymethylation or performic acid oxidation. beta-Thrombin has been found to contain three peptide chains derived by proteolysis of the parent alpha-thrombin molecule. The A chain of alpha-thrombin has been cleaved at two points yielding a peptide (A1 chain) which contains 17 amino acids, beginning with threonine 14 and ending with lysine 30. The B chain of alpha-thrombin has been cleaved at two positions to yield a B1 chain which begins with the NH2-terminal isoleucine and terminates with lysine 65 and a B2 chain which begins with lysine 74 and continues through COOH-terminal serine 259. The A1 chain and B2 chain are linked by a disulfide bridge. Although there is no evidence for a covalent bond between the B1 chain and the B2-A1 chains, the B1 chain is tightly bound to the remainder of the molecule, for separation is achieved only under denaturing conditions.", "contents": "The covalent differences between bovine alpha- and beta-thrombin. A structural explanation for the changes in catalytic activity. The partial covalent structure of bovine beta-thrombin has been determined by the use of automated Edman degradation and carboxypeptidase digestion of the component polypeptide chains separated by gel filtration following either reduction and carboxymethylation or performic acid oxidation. beta-Thrombin has been found to contain three peptide chains derived by proteolysis of the parent alpha-thrombin molecule. The A chain of alpha-thrombin has been cleaved at two points yielding a peptide (A1 chain) which contains 17 amino acids, beginning with threonine 14 and ending with lysine 30. The B chain of alpha-thrombin has been cleaved at two positions to yield a B1 chain which begins with the NH2-terminal isoleucine and terminates with lysine 65 and a B2 chain which begins with lysine 74 and continues through COOH-terminal serine 259. The A1 chain and B2 chain are linked by a disulfide bridge. Although there is no evidence for a covalent bond between the B1 chain and the B2-A1 chains, the B1 chain is tightly bound to the remainder of the molecule, for separation is achieved only under denaturing conditions."}
{"id": "PMID:468840", "title": "11-cis-Retinal-binding protein from bovine retina. Isolation and partial characterization.", "content": "11-cis-Retinal-binding protein from bovine retina has been purified to apparent homogeneity by gel filtration ion exchange, and hydroxylapatite chromatography. The molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 33,000. A value of 36,000 was obtained by gel filtration. With 11-cis-retinal bound to the protein a bleachable spectral peak with maximum absorption at 425 nm was observed. When isolated without addition of exogenous retinal the purified binding protein displayed absorbance maxima at 340 and 425 nm, indicating the occurrence of two ligands in addition to the typical protein absorbance. The endogenous ligands responsible for these maxima were tentatively identified as 11-cis-retinol and 11-cis-retinal, respectively. The binding protein did not cross-react with antibodies prepared against bovine opsin, and its amino acid composition was distinct from that of opsin. The amount of retinal-binding protein extracted was approximately 1 nmol/retina or 1 mol of binding protein/20 mol of opsin. Its specificity and relative abundance suggest that the 11-cis-retinal-binding protein may play a major role in the visual cycle.", "contents": "11-cis-Retinal-binding protein from bovine retina. Isolation and partial characterization. 11-cis-Retinal-binding protein from bovine retina has been purified to apparent homogeneity by gel filtration ion exchange, and hydroxylapatite chromatography. The molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 33,000. A value of 36,000 was obtained by gel filtration. With 11-cis-retinal bound to the protein a bleachable spectral peak with maximum absorption at 425 nm was observed. When isolated without addition of exogenous retinal the purified binding protein displayed absorbance maxima at 340 and 425 nm, indicating the occurrence of two ligands in addition to the typical protein absorbance. The endogenous ligands responsible for these maxima were tentatively identified as 11-cis-retinol and 11-cis-retinal, respectively. The binding protein did not cross-react with antibodies prepared against bovine opsin, and its amino acid composition was distinct from that of opsin. The amount of retinal-binding protein extracted was approximately 1 nmol/retina or 1 mol of binding protein/20 mol of opsin. Its specificity and relative abundance suggest that the 11-cis-retinal-binding protein may play a major role in the visual cycle."}
{"id": "PMID:468841", "title": "The sugar chains of cold-insoluble globulin. A protein related to fibronectin.", "content": "Cold-insoluble globulin isolated from bovine plasma contains six asparagine-linked sugar chains in 1 molecule (a dimeric form). These sugar chains were released from the polypeptide backbone by hydrazinolysis and labeled by reduction with NaB[3H]4. Most of these sugar chains contain N-acetylneuraminic acid and can be separated by paper electrophoresis. By combination of sequential exoglycosidase digestion and methylation study, their structures were elucidated as Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc, NeuAc alpha 2 leads to 6 or 4Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 4 or 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc, NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]-Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc and NeuAc alpha 2 leads to 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc.", "contents": "The sugar chains of cold-insoluble globulin. A protein related to fibronectin. Cold-insoluble globulin isolated from bovine plasma contains six asparagine-linked sugar chains in 1 molecule (a dimeric form). These sugar chains were released from the polypeptide backbone by hydrazinolysis and labeled by reduction with NaB[3H]4. Most of these sugar chains contain N-acetylneuraminic acid and can be separated by paper electrophoresis. By combination of sequential exoglycosidase digestion and methylation study, their structures were elucidated as Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc, NeuAc alpha 2 leads to 6 or 4Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 4 or 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc, NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]-Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc and NeuAc alpha 2 leads to 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc."}
{"id": "PMID:468842", "title": "The biosynthesis of cyanogenic glucosides in higher plants. N-Hydroxytyrosine as an intermediate in the biosynthesis of dhurrin by Sorghum bicolor (Linn) Moench.", "content": "The following compounds were tested as early intermediates in the conversion of tyrosine to p-hydroxymandelonitrile by a microsomal preparation from dark grown sorghum seedlings: p-hydroxyphenylacetamide, 1-nitro-2-p-hydroxyphenylethane, p-hydroxyphenyl-pyruvic acid oxime, tyramine, N-hydroxytyramine, and N-hydroxytyrosine. Of these, only N-hydroxytyrosine was metabolized to p-hydroxymandelonitrile. N-Hydroxytyrosine was produced from L-[U-14C]tyrosine in tracer experiments when unlabeled N-hydroxytyrosine was added as a trap. These data indicate N-hydroxytyrosine as the first intermediate in the biosynthesis of dhurrin, the cyanogenic glucoside of sorghum, and represent the first demonstration of the formation of an alpha-N-hydroxy-amino acid in a biological system. The enzyme system involved in this reaction was partially characterized with respect to substrate specificity and the effect of various inhibitors. The enzyme was shown to have properties different than those reported for the mammalian enzyme system(s) involved in the N-hydroxylation of amine drugs. The possible involvement of N-hydroxyamino acids in the biosynthesis of other secondary plant products is discussed.", "contents": "The biosynthesis of cyanogenic glucosides in higher plants. N-Hydroxytyrosine as an intermediate in the biosynthesis of dhurrin by Sorghum bicolor (Linn) Moench. The following compounds were tested as early intermediates in the conversion of tyrosine to p-hydroxymandelonitrile by a microsomal preparation from dark grown sorghum seedlings: p-hydroxyphenylacetamide, 1-nitro-2-p-hydroxyphenylethane, p-hydroxyphenyl-pyruvic acid oxime, tyramine, N-hydroxytyramine, and N-hydroxytyrosine. Of these, only N-hydroxytyrosine was metabolized to p-hydroxymandelonitrile. N-Hydroxytyrosine was produced from L-[U-14C]tyrosine in tracer experiments when unlabeled N-hydroxytyrosine was added as a trap. These data indicate N-hydroxytyrosine as the first intermediate in the biosynthesis of dhurrin, the cyanogenic glucoside of sorghum, and represent the first demonstration of the formation of an alpha-N-hydroxy-amino acid in a biological system. The enzyme system involved in this reaction was partially characterized with respect to substrate specificity and the effect of various inhibitors. The enzyme was shown to have properties different than those reported for the mammalian enzyme system(s) involved in the N-hydroxylation of amine drugs. The possible involvement of N-hydroxyamino acids in the biosynthesis of other secondary plant products is discussed."}
{"id": "PMID:468844", "title": "Subcellular site and mechanism of synthesis of disaturated phosphatidylcholine in alveolar type II cell adenomas.", "content": "The site of synthesis of 1,2-disaturated-(diacyl)-sn-glycero-3-phosphocholine (Sat2PC) in mouse alveolar type II cell adenomas has been studied by conducting pulse-chase experiments. Isolation of microsomal and lamellar body fractions from adenomas after a 20-min pulse with [methyl-3H]choline demonstrates that Sat2PC first appears in the microsomal fraction, and after a short lag subsequently appears in the lamellar body fraction. The kinetics of labeling of Sat2PC are consistent with the microsomal membranes functioning as the subcellular site of synthesis for this pulmonary surfactant phospholipid. Short term labeling experiments with [9,10-3H]palmitate demonstrate that this fatty acid is incorporated into the sn-2 position of Sat2PC at a faster rate than its incorporation into the sn-1 position. This finding indicates that the synthesis of Sat2PC occurs by a deacylation-reacylation mechanism.", "contents": "Subcellular site and mechanism of synthesis of disaturated phosphatidylcholine in alveolar type II cell adenomas. The site of synthesis of 1,2-disaturated-(diacyl)-sn-glycero-3-phosphocholine (Sat2PC) in mouse alveolar type II cell adenomas has been studied by conducting pulse-chase experiments. Isolation of microsomal and lamellar body fractions from adenomas after a 20-min pulse with [methyl-3H]choline demonstrates that Sat2PC first appears in the microsomal fraction, and after a short lag subsequently appears in the lamellar body fraction. The kinetics of labeling of Sat2PC are consistent with the microsomal membranes functioning as the subcellular site of synthesis for this pulmonary surfactant phospholipid. Short term labeling experiments with [9,10-3H]palmitate demonstrate that this fatty acid is incorporated into the sn-2 position of Sat2PC at a faster rate than its incorporation into the sn-1 position. This finding indicates that the synthesis of Sat2PC occurs by a deacylation-reacylation mechanism."}
{"id": "PMID:468845", "title": "Purification of a high molecular weight human terminal deoxynucleotidyl transferase.", "content": "Terminal deoxynucleotidyltransferase has been purified from lymphoblasts of leukemic patients. The enzyme has a molecular weight of approximately 62,000 as determined by gel filtration and nondenaturing gel electrophoresis and is not dissociated into subunits by sodium dodecyl sulfate. In contrast, the terminal transferase enzyme from calf thymus has a molecular weight of 42,000 as determined by gel filtration, and is dissociated into 2 subunits of Mr 30,000 and 8,000 by sodium dodecyl sulfate. The enzyme has an isoelectric point of 8.2 and kinetic characteristics which are similar to those of calf thymus terminal transferase. The apparent Km for purine nucleotide polymerization at saturating initiator concentration with Mg2+ is 0.2 mM and with Mn2+ is 0.05 mM. Like calf terminal transferase, the reaction velocity is higher in the presence of Mg2+ than Mn2+. ATP inhibits the reaction catalyzed by terminal transferase isolated from human lymphoblasts due to mutual recognition of ATP and dATP by a common site on the enzyme. Preliminary experiments indicate that human terminal transferase may contain a small amount of carbohydrate. This report represents the first purification to near homogeneity of terminal transferase from a tissue source other than calf thymus.", "contents": "Purification of a high molecular weight human terminal deoxynucleotidyl transferase. Terminal deoxynucleotidyltransferase has been purified from lymphoblasts of leukemic patients. The enzyme has a molecular weight of approximately 62,000 as determined by gel filtration and nondenaturing gel electrophoresis and is not dissociated into subunits by sodium dodecyl sulfate. In contrast, the terminal transferase enzyme from calf thymus has a molecular weight of 42,000 as determined by gel filtration, and is dissociated into 2 subunits of Mr 30,000 and 8,000 by sodium dodecyl sulfate. The enzyme has an isoelectric point of 8.2 and kinetic characteristics which are similar to those of calf thymus terminal transferase. The apparent Km for purine nucleotide polymerization at saturating initiator concentration with Mg2+ is 0.2 mM and with Mn2+ is 0.05 mM. Like calf terminal transferase, the reaction velocity is higher in the presence of Mg2+ than Mn2+. ATP inhibits the reaction catalyzed by terminal transferase isolated from human lymphoblasts due to mutual recognition of ATP and dATP by a common site on the enzyme. Preliminary experiments indicate that human terminal transferase may contain a small amount of carbohydrate. This report represents the first purification to near homogeneity of terminal transferase from a tissue source other than calf thymus."}
{"id": "PMID:468846", "title": "Identification by affinity chromatography of the eukaryotic ribosomal proteins that bind to 5.8 S ribosomal ribonucleic acid.", "content": "The proteins that bind to rat liver 5.8 S ribosomal ribonucleic acid were identified by affinity chromatography. The nucleic acid was oxidized with periodate and coupled by its 3'-terminus to Sepharose 4B through and adipic acid dihydrazide spacer. The ribosomal proteins that associate with the immobilized 5.8 S rRNA were identified by polyacrylamide gel electrophoresiss: they were L19, L8, and L6 from the 60 S subunit; and S13 and S9 from the small subparticle. Small amounts of L14, L17', L18, L27/L27', and L35', and of S11, S15, S23/S24, and S26 also were bound to the affinity column, but whether they associate directly and specifically with 5.8 S rRNA is not known. Escherichia coli ribosomal proteins did not bind to the rat liver 5.8 S rRNA affinity column.", "contents": "Identification by affinity chromatography of the eukaryotic ribosomal proteins that bind to 5.8 S ribosomal ribonucleic acid. The proteins that bind to rat liver 5.8 S ribosomal ribonucleic acid were identified by affinity chromatography. The nucleic acid was oxidized with periodate and coupled by its 3'-terminus to Sepharose 4B through and adipic acid dihydrazide spacer. The ribosomal proteins that associate with the immobilized 5.8 S rRNA were identified by polyacrylamide gel electrophoresiss: they were L19, L8, and L6 from the 60 S subunit; and S13 and S9 from the small subparticle. Small amounts of L14, L17', L18, L27/L27', and L35', and of S11, S15, S23/S24, and S26 also were bound to the affinity column, but whether they associate directly and specifically with 5.8 S rRNA is not known. Escherichia coli ribosomal proteins did not bind to the rat liver 5.8 S rRNA affinity column."}
{"id": "PMID:468868", "title": "Abdominal biopsy with ultrasound guidance.", "content": "The value of aspiration biopsy under ultrasound guidance has been demonstrated in 51 patients. The confirmation of malignancy, particularly, has allowed many patients the value of chemotherapy or a quiet demise, without the necessity for diagnostic laparotomy.", "contents": "Abdominal biopsy with ultrasound guidance. The value of aspiration biopsy under ultrasound guidance has been demonstrated in 51 patients. The confirmation of malignancy, particularly, has allowed many patients the value of chemotherapy or a quiet demise, without the necessity for diagnostic laparotomy."}
{"id": "PMID:468869", "title": "Percutaneous puncture of abdominal cystic masses in children.", "content": "A technique of percutaneous puncture and opacification of cystic abdominal masses is outlined, and its diagnostic and therapeutic potential demonstrated in a series of 16 masses in 15 children. It is suggested as an alternative to ultrasound and computed tomography in certain situations.", "contents": "Percutaneous puncture of abdominal cystic masses in children. A technique of percutaneous puncture and opacification of cystic abdominal masses is outlined, and its diagnostic and therapeutic potential demonstrated in a series of 16 masses in 15 children. It is suggested as an alternative to ultrasound and computed tomography in certain situations."}
{"id": "PMID:468870", "title": "Percutaneous pulmonary aspiration biopsy using the Chiba needle.", "content": "The Chiba needle was used for percutaneous needle aspiration of lung lesions in 35 patients. Two separate needles were used for each patient at the same sitting. The aspirate was true positive for malignancy in 23 patients and true negative for malignancy in eight patients. Two aspirates were false negative for malignancy and two aspirates, negative for malignancy, were classified as inconclusive due to inadequate patient follow-up. The overall accuracy rate was 94%. Six patients had small pneumothoraces but only one required a chest tube. Appreciable hemoptysis did not occur. The Chiba needle provided an adequate aspirate for cytologic diagnosis. The complication rate using two needles appears to be entirely acceptable.", "contents": "Percutaneous pulmonary aspiration biopsy using the Chiba needle. The Chiba needle was used for percutaneous needle aspiration of lung lesions in 35 patients. Two separate needles were used for each patient at the same sitting. The aspirate was true positive for malignancy in 23 patients and true negative for malignancy in eight patients. Two aspirates were false negative for malignancy and two aspirates, negative for malignancy, were classified as inconclusive due to inadequate patient follow-up. The overall accuracy rate was 94%. Six patients had small pneumothoraces but only one required a chest tube. Appreciable hemoptysis did not occur. The Chiba needle provided an adequate aspirate for cytologic diagnosis. The complication rate using two needles appears to be entirely acceptable."}
{"id": "PMID:468871", "title": "[Transhepatic percutaneous cholangiography: A comparative study].", "content": "A retrospective study of 75 cases (19 with the conventional method and 56 with the Chiba method) of percutaneous transhepatic cholangiography was done. The utilization of the Chiba method is associated with a higher success rate of opacification of the biliary tree: 97% versus 80% for dilated biliary ducts and 36% versus 0% for non-dilated biliary ducts. No serious complications were encountered with the Chiba method. With the utilization of the \"skinny\" Chiba needle, percutaneous transhepatic cholangiography has become a simple, safe, and efficient method in the investigation of hepatobiliary disorders.", "contents": "[Transhepatic percutaneous cholangiography: A comparative study]. A retrospective study of 75 cases (19 with the conventional method and 56 with the Chiba method) of percutaneous transhepatic cholangiography was done. The utilization of the Chiba method is associated with a higher success rate of opacification of the biliary tree: 97% versus 80% for dilated biliary ducts and 36% versus 0% for non-dilated biliary ducts. No serious complications were encountered with the Chiba method. With the utilization of the \"skinny\" Chiba needle, percutaneous transhepatic cholangiography has become a simple, safe, and efficient method in the investigation of hepatobiliary disorders."}
{"id": "PMID:468872", "title": "Hydatidiform mole with co-existent fetus.", "content": "Co-existence of hydatidiform mole and fetus is a rare condition, but one in which the radiologist, through ultrasound, can play an important role in diagnosis. The typical patient, with no pre-existing hypertension or renal disease, develops severe pre-eclampsia in the second trimester with elevated gonadotropin levels. Ultrasound shows a large placenta containing varying numbers of sonolucent spaces, associated with a live or dead fetus. This condition appears to be a separate entity from classical hydatidiform mole both pathologically and prognostically. It should also be differentiated from simple hydatidiform swelling which does not have the associated clinical picture.", "contents": "Hydatidiform mole with co-existent fetus. Co-existence of hydatidiform mole and fetus is a rare condition, but one in which the radiologist, through ultrasound, can play an important role in diagnosis. The typical patient, with no pre-existing hypertension or renal disease, develops severe pre-eclampsia in the second trimester with elevated gonadotropin levels. Ultrasound shows a large placenta containing varying numbers of sonolucent spaces, associated with a live or dead fetus. This condition appears to be a separate entity from classical hydatidiform mole both pathologically and prognostically. It should also be differentiated from simple hydatidiform swelling which does not have the associated clinical picture."}
{"id": "PMID:468873", "title": "Misleading skeletal surveys of prostatic carcinoma.", "content": "Bone sclerosis can be a manifestation of regressing metastatic bone disease of carcinoma of the prostate and should not be misconstrued as further dissemination. In eight of our patients the skeletal survey radiographs were misleading, whereas the bone scan provided an accurate assessment of metastatic activity.", "contents": "Misleading skeletal surveys of prostatic carcinoma. Bone sclerosis can be a manifestation of regressing metastatic bone disease of carcinoma of the prostate and should not be misconstrued as further dissemination. In eight of our patients the skeletal survey radiographs were misleading, whereas the bone scan provided an accurate assessment of metastatic activity."}
{"id": "PMID:468874", "title": "Pulmonary embolism with unilateral lung scan defects and matching infiltrates.", "content": "Ten patients with matching ventilation-perfusion lung scan defects and corresponding pulmonary infiltrates were evaluated with segmental pulmonary angiography. All ten patients presented with sudden onset of pleuritic chest pain and fever. Pulmonary emboli were documented in three of the ten patients (30%). The remaining seven patients had pneumonia or atelectasis. The findings emphasize the non-diagnostic nature of lung scans which show only matching ventilation and perfusion defects in regions of pulmonary infiltrates. Segmental pulmonary angiography is recommended for differentiating pulmonary embolism from atelectasis or pneumonia in these patients.", "contents": "Pulmonary embolism with unilateral lung scan defects and matching infiltrates. Ten patients with matching ventilation-perfusion lung scan defects and corresponding pulmonary infiltrates were evaluated with segmental pulmonary angiography. All ten patients presented with sudden onset of pleuritic chest pain and fever. Pulmonary emboli were documented in three of the ten patients (30%). The remaining seven patients had pneumonia or atelectasis. The findings emphasize the non-diagnostic nature of lung scans which show only matching ventilation and perfusion defects in regions of pulmonary infiltrates. Segmental pulmonary angiography is recommended for differentiating pulmonary embolism from atelectasis or pneumonia in these patients."}
{"id": "PMID:468875", "title": "Gallium-67 citrate in detecting recurrent rectal carcinoma after abdomino-perineal resection.", "content": "In a retrospective study we have found gallium-67 citrate imaging of the abdomen to be a value in detecting pelvic recurrence of carcinoma of the rectum after abdomino-perineal excision. In this context the technique proved to have an accuracy of 83%, sensitivity of 82% and specificity of 83%.", "contents": "Gallium-67 citrate in detecting recurrent rectal carcinoma after abdomino-perineal resection. In a retrospective study we have found gallium-67 citrate imaging of the abdomen to be a value in detecting pelvic recurrence of carcinoma of the rectum after abdomino-perineal excision. In this context the technique proved to have an accuracy of 83%, sensitivity of 82% and specificity of 83%."}
{"id": "PMID:468876", "title": "Astrocytoma in children. A review of 79 cases--1960-1976.", "content": "Seventy-nine children with histologically proven astrocytomas, 32 cerebral, 30 cerebellar, 9 thalamic, 6 optic nerve, and 2 in the 4th ventricular floor were reviewed retrospectively. Seventy-one per cent of the astrocytomas were well differentiated. Unlike adult astrocytomas, there was slight female predominance in our group. Cerebral astrocytomas were more frequent beyond nine years of age. The overall five-year survival rate of cerebral astrocytomas was 95% (19/20) for well-differentiated, and 19% (2/12) for poorly-differentiated tumors. In cerebellar astrocytomas the survival rates were 75% (18/24) and 33% (2/6) respectively.", "contents": "Astrocytoma in children. A review of 79 cases--1960-1976. Seventy-nine children with histologically proven astrocytomas, 32 cerebral, 30 cerebellar, 9 thalamic, 6 optic nerve, and 2 in the 4th ventricular floor were reviewed retrospectively. Seventy-one per cent of the astrocytomas were well differentiated. Unlike adult astrocytomas, there was slight female predominance in our group. Cerebral astrocytomas were more frequent beyond nine years of age. The overall five-year survival rate of cerebral astrocytomas was 95% (19/20) for well-differentiated, and 19% (2/12) for poorly-differentiated tumors. In cerebellar astrocytomas the survival rates were 75% (18/24) and 33% (2/6) respectively."}
{"id": "PMID:468877", "title": "Pitfalls to avoid: spasm in ulcerative colitis masquerading as carcinoma.", "content": "A barium enema on a patient with chronic ulcerative colitis demonstrated a stricture with the radiographic characteristics of an annular carcinoma. The \"lesion\" disappeared following administration of glucagon.", "contents": "Pitfalls to avoid: spasm in ulcerative colitis masquerading as carcinoma. A barium enema on a patient with chronic ulcerative colitis demonstrated a stricture with the radiographic characteristics of an annular carcinoma. The \"lesion\" disappeared following administration of glucagon."}
{"id": "PMID:468878", "title": "Technical note: oral water and intravenous glucagon--to aid ultrasonic visualization of the common bile duct.", "content": "Gas in the stomach, duodenum and hepatic flexure frequently prevents ultrasonic visualization of the distal common bile duct. By filling the stomach and duodenum with water, enhanced by temporary paralysis with glucagon, the gas is displaced and improved visualization of the distal common bile duct is obtained.", "contents": "Technical note: oral water and intravenous glucagon--to aid ultrasonic visualization of the common bile duct. Gas in the stomach, duodenum and hepatic flexure frequently prevents ultrasonic visualization of the distal common bile duct. By filling the stomach and duodenum with water, enhanced by temporary paralysis with glucagon, the gas is displaced and improved visualization of the distal common bile duct is obtained."}
{"id": "PMID:468879", "title": "Congenital lipoid nephrosis with left renal vein thrombosis and Chiari's syndrome.", "content": "An infant with primary congenital lipoid nephrosis then developed left renal vein thrombosis and secondary hepatic vein obstruction. This was shown by inferior venacavography. The thrombus detached subsequently, and the child died from massive pulmonary embolism.", "contents": "Congenital lipoid nephrosis with left renal vein thrombosis and Chiari's syndrome. An infant with primary congenital lipoid nephrosis then developed left renal vein thrombosis and secondary hepatic vein obstruction. This was shown by inferior venacavography. The thrombus detached subsequently, and the child died from massive pulmonary embolism."}
{"id": "PMID:468880", "title": "Arteriographic demonstration of extension of renal carcinoma into renal vein and vena cava.", "content": "On rare occasions, arteriography of a renal cell carcinoma shows tumor vessels following the course of the renal vein and vena cava. In our cases, surgical exploration and microscopic examination showed that this appearance  was due to tumor vessels arising from within the renal tumor and extending intraluminally within the venous system to supply the tumor.", "contents": "Arteriographic demonstration of extension of renal carcinoma into renal vein and vena cava. On rare occasions, arteriography of a renal cell carcinoma shows tumor vessels following the course of the renal vein and vena cava. In our cases, surgical exploration and microscopic examination showed that this appearance  was due to tumor vessels arising from within the renal tumor and extending intraluminally within the venous system to supply the tumor."}
{"id": "PMID:468881", "title": "Legionnaires' disease in Vancouver.", "content": "A female patient presented at the end of a holiday cruise with the pneumonitis of Legionnaires' disease. The radiographic appearance was indistinguishable from any other cause of air-space consolidation.", "contents": "Legionnaires' disease in Vancouver. A female patient presented at the end of a holiday cruise with the pneumonitis of Legionnaires' disease. The radiographic appearance was indistinguishable from any other cause of air-space consolidation."}
{"id": "PMID:468882", "title": "Ultrasound findings in small bowel infarction.", "content": "A patient with small bowel infarction was studied using grey-scale ultrasound. The appearance was that of a lobulated abdominal mass consisting of smaller masses with a central sonolucent area and a peripheral echogenic rind. This is the reverse of what has been described in intramural intestinal hematoma, intussusception, and inflammatory and neoplastic bowel masses.", "contents": "Ultrasound findings in small bowel infarction. A patient with small bowel infarction was studied using grey-scale ultrasound. The appearance was that of a lobulated abdominal mass consisting of smaller masses with a central sonolucent area and a peripheral echogenic rind. This is the reverse of what has been described in intramural intestinal hematoma, intussusception, and inflammatory and neoplastic bowel masses."}
{"id": "PMID:468883", "title": "Abdominal hematomas in unusual locations--contribution of ultrasound.", "content": "The ultrasonic findings in two patients with abdominal hematomas in unusual locations, gallbladder and mesocolon, are described. When integrated with radiologic imaging methods, ultrasound played a vital role.", "contents": "Abdominal hematomas in unusual locations--contribution of ultrasound. The ultrasonic findings in two patients with abdominal hematomas in unusual locations, gallbladder and mesocolon, are described. When integrated with radiologic imaging methods, ultrasound played a vital role."}
{"id": "PMID:468884", "title": "Microsomal mediated metabolism of dialkylaryltriazenes. I. Demethylation of ring halogenated 3,3-dimethyl-1-phenyltriazenes.", "content": "The oxidative N-demethylation was investigated for a series of 3,3-dimethyl-l-phenyl-triazenes. Triazenes, deactivated with halogene atoms in the phenylring, were expected to be better demethylated. The results do indicate a good trend that substitution of the ring with deactivating atoms and extent of demethylation compare well. The percentages of demethylation were: For 3,3-dimethyl-l-phenyltriazene, 45%; for 3,3-dimethyl-l (4-chlor-phenyl)-triazene, 92%; for 3,3-dimethyl-l(4-bromophenyl)triazene, 89%; for 3,3-dimethyl-l-(2,4,6-trichlorophenyl)triazene, 122%; and for 3,3-dimethyl-l-l-(2,4,6-tribromophenyl)triazene, 85%.", "contents": "Microsomal mediated metabolism of dialkylaryltriazenes. I. Demethylation of ring halogenated 3,3-dimethyl-1-phenyltriazenes. The oxidative N-demethylation was investigated for a series of 3,3-dimethyl-l-phenyl-triazenes. Triazenes, deactivated with halogene atoms in the phenylring, were expected to be better demethylated. The results do indicate a good trend that substitution of the ring with deactivating atoms and extent of demethylation compare well. The percentages of demethylation were: For 3,3-dimethyl-l-phenyltriazene, 45%; for 3,3-dimethyl-l (4-chlor-phenyl)-triazene, 92%; for 3,3-dimethyl-l(4-bromophenyl)triazene, 89%; for 3,3-dimethyl-l-(2,4,6-trichlorophenyl)triazene, 122%; and for 3,3-dimethyl-l-l-(2,4,6-tribromophenyl)triazene, 85%."}
{"id": "PMID:468885", "title": "Microsomal mediated metabolism of dialkylaryltriazenes. II. Isolation and identification of metabolites of 3,3-dimethyl-1-phenyltriazene.", "content": "After incubating 3,3-dimethyl-1-phenyltriazene with rat liver microsomes, acetanilid and derivatives of aniline and possibly of 3-methyl-1-phenyltriazene were found as metabolites and identified by mass spectrometry. This is the first time that directly formed metabolites (other than formaldehyde) of a dialkyltriazene were identified. The isolation of a derivative of 3-methyl-1-phenyltriazene as 3-acetyl-3-methyl-1-phenyltriazene supports other evidence that the triazenes are enzymically demethylated. Indication for the formation of phenylhydrazine was also obtained. In addition, hydrolysates of the polar fractions of the incubation mixture contained aniline and 4-hydroxy-aniline as a aglycones.", "contents": "Microsomal mediated metabolism of dialkylaryltriazenes. II. Isolation and identification of metabolites of 3,3-dimethyl-1-phenyltriazene. After incubating 3,3-dimethyl-1-phenyltriazene with rat liver microsomes, acetanilid and derivatives of aniline and possibly of 3-methyl-1-phenyltriazene were found as metabolites and identified by mass spectrometry. This is the first time that directly formed metabolites (other than formaldehyde) of a dialkyltriazene were identified. The isolation of a derivative of 3-methyl-1-phenyltriazene as 3-acetyl-3-methyl-1-phenyltriazene supports other evidence that the triazenes are enzymically demethylated. Indication for the formation of phenylhydrazine was also obtained. In addition, hydrolysates of the polar fractions of the incubation mixture contained aniline and 4-hydroxy-aniline as a aglycones."}
{"id": "PMID:468886", "title": "[Fluorometric determination of \"activated\" cyclophosphamide and ifosfamide in blood (author's transl)].", "content": "\"Activated\" N-(2-Chloroethyl)amido-oxazaphosphorines like 4-hydroxycyclophosphamide, 4-hydroperoxycyclophosphamide, 4-hydroxyifosfamide, and 4-hydroperoxyifosfamide can be determined fluorometrically by condensation of liberated acrolein with m-aminophenol yielding 7-hydroxychinolin. The method permits determination of 10(-10) mol and is specific for \"activated\" N-(2-Chloroethyl)amido-oxazaphosphorine metabolites which liberate acrolein under conditions of the test. Neither cyclophosphamide nor ifosfamide or other metabolites of this cytostatics interfere with the test. Blood levels of free 4-hydroxycyclophosphamide and 4-hydroxyifosfamide were determined after injection of cyclophosphamide and ifosfamide into mice.", "contents": "[Fluorometric determination of \"activated\" cyclophosphamide and ifosfamide in blood (author's transl)]. \"Activated\" N-(2-Chloroethyl)amido-oxazaphosphorines like 4-hydroxycyclophosphamide, 4-hydroperoxycyclophosphamide, 4-hydroxyifosfamide, and 4-hydroperoxyifosfamide can be determined fluorometrically by condensation of liberated acrolein with m-aminophenol yielding 7-hydroxychinolin. The method permits determination of 10(-10) mol and is specific for \"activated\" N-(2-Chloroethyl)amido-oxazaphosphorine metabolites which liberate acrolein under conditions of the test. Neither cyclophosphamide nor ifosfamide or other metabolites of this cytostatics interfere with the test. Blood levels of free 4-hydroxycyclophosphamide and 4-hydroxyifosfamide were determined after injection of cyclophosphamide and ifosfamide into mice."}
{"id": "PMID:468887", "title": "Malignant progression of angioimmunoblastic lymphadenopathy.", "content": "In a 79-year-old woman, the progression of angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) to malignant lymphoma was observed within one year after diagnosis. Three biopsy specimens from lymph nodes and one tonsil, obtained at intervals of several months, showed an increasing destruction of the tissue architecture and the development of histological criteria for a lymphoid neoplasm, which at autopsy was confirmed as a malignant non-Hodgkin's lymphoma. The demonstration of a chromosomally abnormal clone in lymph node derived and the laboratory findings were in agreement with the histological changes and the sequential clinical deterioation. Initially, a symptom-free interval of eight months was achieved with prednisone therapy. However, this treatment failed after the malignant transformation had become evident.", "contents": "Malignant progression of angioimmunoblastic lymphadenopathy. In a 79-year-old woman, the progression of angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) to malignant lymphoma was observed within one year after diagnosis. Three biopsy specimens from lymph nodes and one tonsil, obtained at intervals of several months, showed an increasing destruction of the tissue architecture and the development of histological criteria for a lymphoid neoplasm, which at autopsy was confirmed as a malignant non-Hodgkin's lymphoma. The demonstration of a chromosomally abnormal clone in lymph node derived and the laboratory findings were in agreement with the histological changes and the sequential clinical deterioation. Initially, a symptom-free interval of eight months was achieved with prednisone therapy. However, this treatment failed after the malignant transformation had become evident."}
{"id": "PMID:468888", "title": "Morphometric and cytophotometric investigations of lobular neoplasia of the breast with ductal involvement.", "content": "The two different types, A and B, of in situ lobular neoplasia of the breast can be distinguished by cytomorphological means. DNA-measurements of type A are preponderantly diploid while those of type B vary from diploidy to hyperteraploidy with a maximum in the hyperdiploid range. The results indicate a higher potential of malignancy in type B. Homogenous ductular cell proliferations in the immediate neighbourhood of a lobular neoplasia display virtually the same distribution of DNA-values. Such evidence suggests that ductular changes and concomitant intralobular lesions have a common genesis, showing also a similar tendency towards malignant changes.", "contents": "Morphometric and cytophotometric investigations of lobular neoplasia of the breast with ductal involvement. The two different types, A and B, of in situ lobular neoplasia of the breast can be distinguished by cytomorphological means. DNA-measurements of type A are preponderantly diploid while those of type B vary from diploidy to hyperteraploidy with a maximum in the hyperdiploid range. The results indicate a higher potential of malignancy in type B. Homogenous ductular cell proliferations in the immediate neighbourhood of a lobular neoplasia display virtually the same distribution of DNA-values. Such evidence suggests that ductular changes and concomitant intralobular lesions have a common genesis, showing also a similar tendency towards malignant changes."}
{"id": "PMID:468889", "title": "Tumor host relations. I. Increased plasma cortisol in tumor-bearing humans compared with patients with benign surgical diseases.", "content": "Plasma cortisol was measured by a protein binding technique in 81 patients with malignant tumors of different extent and various sites and in 82 patients with benign surgical diseases. The mean value of the tumor patients (x +/- s = 165 +/- 69 micrograms cortisol/l plasma) was increased significantly compared with the benign surgical disorders (100 +/- 45 micrograms/l). Within the group of patients with benign surgical disorders there was little variation by the type of disease (cortisol mean values given in brackets): benign breast tumors (95), gall stones (107), ulcer of the stomach and duodenum (96), hernia (78), appendicitis acuta (112), and struma (90). The results are in accordance with the hypothesis that glucocorticoids are involved in the increased protein catabolism of skeletal muscles and other signs of cachectic tumor patients.", "contents": "Tumor host relations. I. Increased plasma cortisol in tumor-bearing humans compared with patients with benign surgical diseases. Plasma cortisol was measured by a protein binding technique in 81 patients with malignant tumors of different extent and various sites and in 82 patients with benign surgical diseases. The mean value of the tumor patients (x +/- s = 165 +/- 69 micrograms cortisol/l plasma) was increased significantly compared with the benign surgical disorders (100 +/- 45 micrograms/l). Within the group of patients with benign surgical disorders there was little variation by the type of disease (cortisol mean values given in brackets): benign breast tumors (95), gall stones (107), ulcer of the stomach and duodenum (96), hernia (78), appendicitis acuta (112), and struma (90). The results are in accordance with the hypothesis that glucocorticoids are involved in the increased protein catabolism of skeletal muscles and other signs of cachectic tumor patients."}
{"id": "PMID:468890", "title": "Tumor host relations. II. Influence of tumor extent and tumor site on plasma cortisol of patients with malignant diseases.", "content": "The increase of plasma cortisol in patients with tumors of five different sites compared with a control group of patients with benign surgical diseases amounted to: +39% (breast), +34% (stomach), +86% (intestine), +60% (skin) and +194% (gall bladder). The first detectable increase of cortisol occurred in patients with tumors classified T 2 according to the TNM scheme (+27% above the control). Highly significant increases were observed for T 3 (+82%) and T 4 (+77%) patients. Patients with palpable lymph nodes showed a most significantly increased cortisol mean value compared with patients without palpable lymph nodes. Similarly, the cortisol mean value of patients with distant metastases was significantly higher than the corresponding value of tumor patients without distant metastases. The question remains open, whether the primary site, the extent of the tumor or the occurrence of metastases is the main determinant for the cortisol increase.", "contents": "Tumor host relations. II. Influence of tumor extent and tumor site on plasma cortisol of patients with malignant diseases. The increase of plasma cortisol in patients with tumors of five different sites compared with a control group of patients with benign surgical diseases amounted to: +39% (breast), +34% (stomach), +86% (intestine), +60% (skin) and +194% (gall bladder). The first detectable increase of cortisol occurred in patients with tumors classified T 2 according to the TNM scheme (+27% above the control). Highly significant increases were observed for T 3 (+82%) and T 4 (+77%) patients. Patients with palpable lymph nodes showed a most significantly increased cortisol mean value compared with patients without palpable lymph nodes. Similarly, the cortisol mean value of patients with distant metastases was significantly higher than the corresponding value of tumor patients without distant metastases. The question remains open, whether the primary site, the extent of the tumor or the occurrence of metastases is the main determinant for the cortisol increase."}
{"id": "PMID:468892", "title": "The incidence of gastro-intestinal cancer in North Baden (West Germany) 1971--1977.", "content": "The incidences of the cancer of the oesophagus, stomach, small intestines, colon, and rectum for the years 1971--1977 in the regional district of North Baden, Federal Republic of Germany are presented and their relation to environmental factors are discussed briefly. The age specific incidence as well as the age standardized incidence and the sex ratios show remarkable differences due to the specific tumor localizations. The colon cancer incidence in the female population increases steeply during the period considered. The risk of developing rectal cancer also increases. The results of the pathoanatomic registry are well comparable with the data from clinical registries.", "contents": "The incidence of gastro-intestinal cancer in North Baden (West Germany) 1971--1977. The incidences of the cancer of the oesophagus, stomach, small intestines, colon, and rectum for the years 1971--1977 in the regional district of North Baden, Federal Republic of Germany are presented and their relation to environmental factors are discussed briefly. The age specific incidence as well as the age standardized incidence and the sex ratios show remarkable differences due to the specific tumor localizations. The colon cancer incidence in the female population increases steeply during the period considered. The risk of developing rectal cancer also increases. The results of the pathoanatomic registry are well comparable with the data from clinical registries."}
{"id": "PMID:468893", "title": "Promoting action of croton oil on gastrocarcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine in rats.", "content": "The promoting effect of croton oil on gastrocarcinogenesis by MNNG was examined in male Wistar rats. Gastric carcinomas were found in five of 10 rats given 83 micrograms/ml MNNG for three months and then 0.02% croton oil with 0.5% Tween 60 as solvent for nine months. No gastric carcinomas were found in rats given MNNG for three months and then Tween 60 only for nine months. The incidence of gastric carcinomas in these two groups was significantly different (p less than 0.05). No tumors were found in rats given only croton oil with Tween 60.", "contents": "Promoting action of croton oil on gastrocarcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine in rats. The promoting effect of croton oil on gastrocarcinogenesis by MNNG was examined in male Wistar rats. Gastric carcinomas were found in five of 10 rats given 83 micrograms/ml MNNG for three months and then 0.02% croton oil with 0.5% Tween 60 as solvent for nine months. No gastric carcinomas were found in rats given MNNG for three months and then Tween 60 only for nine months. The incidence of gastric carcinomas in these two groups was significantly different (p less than 0.05). No tumors were found in rats given only croton oil with Tween 60."}
{"id": "PMID:468894", "title": "Comparative studies of neoplastic response to a single dose of nitroso compounds. 6. The effect of diethylnitrosamine in Syrian golden hamsters.", "content": "The effect of a single subcutaneous injection of different DEN doses was examined in adult Syrian hamsters observed for life. The minimal effective dose (threshold dose) for neoplastic response, reflected by induction of papillary polyps in the larynx and/or trachea, was 1.03 mg DEN/kg body weight. No tumors were found which could be related to treatment in other segments of the respiratory epithelium nor in other tissues.", "contents": "Comparative studies of neoplastic response to a single dose of nitroso compounds. 6. The effect of diethylnitrosamine in Syrian golden hamsters. The effect of a single subcutaneous injection of different DEN doses was examined in adult Syrian hamsters observed for life. The minimal effective dose (threshold dose) for neoplastic response, reflected by induction of papillary polyps in the larynx and/or trachea, was 1.03 mg DEN/kg body weight. No tumors were found which could be related to treatment in other segments of the respiratory epithelium nor in other tissues."}
{"id": "PMID:468895", "title": "Implantation of cigarette smoke condensate in the lungs of Syrian golden hamsters.", "content": "Different doses of benzo(a)pyrene, cigarette smoke condensate (CSC) and its nitromethane fraction (NMF), in wax pellets, were directly implanted into the lungs of Syrian golden hamsters. Pleomorphic sarcomas were induced in the B(a)P treated animals. Several epithelial proliferative lesions and a few bronchiogenic adenomas were induced in CSC and NMF treated hamsters. A species-specific effect has been observed in this investigation.", "contents": "Implantation of cigarette smoke condensate in the lungs of Syrian golden hamsters. Different doses of benzo(a)pyrene, cigarette smoke condensate (CSC) and its nitromethane fraction (NMF), in wax pellets, were directly implanted into the lungs of Syrian golden hamsters. Pleomorphic sarcomas were induced in the B(a)P treated animals. Several epithelial proliferative lesions and a few bronchiogenic adenomas were induced in CSC and NMF treated hamsters. A species-specific effect has been observed in this investigation."}
{"id": "PMID:468896", "title": "Effect of liposome-entrapped methotrexate on Ehrlich ascites tumor cells and uptake in primary liver cell tumor.", "content": "A therapeutically useful concentration (0,5 mg/ml) of Methotrexate was prepared in negatively charged artificial liposomes (lecithine: cholesterol: dicetylphosphate=5:5:1 molar ratios). Entrapment yield after separation on Sepharose 6B is nearly 100%. In contrast to free Methotrexate the liposome entrapped folic acid antagonist is eliminated only slowly by the kidneys and after intravenous application it is unevenly distributed in the body of mice, the highest concentrations being found in liver and spleen. Daily injections (7.5 mg/kg/day) of entrapped Methotrexate for 5 days into the tail vein of Ehrlich ascites tumor bearing mice reduced both tumor cell count and the production of ascites fluid about fourfold as compared to mice receiving the same dose of Methotrexate in the free form. Six hours after intravenous application of liposome entrapped Methotrexate the tissue concentration in normal liver is ca. 20-fold higher than when the same amount is applied in the free state. On the other hand, only a little uptake of liposome entrapped Methotrexate was detected in the tissue of nitrosamine-induced primary liver tumor when compared to normal liver tissue of rats.", "contents": "Effect of liposome-entrapped methotrexate on Ehrlich ascites tumor cells and uptake in primary liver cell tumor. A therapeutically useful concentration (0,5 mg/ml) of Methotrexate was prepared in negatively charged artificial liposomes (lecithine: cholesterol: dicetylphosphate=5:5:1 molar ratios). Entrapment yield after separation on Sepharose 6B is nearly 100%. In contrast to free Methotrexate the liposome entrapped folic acid antagonist is eliminated only slowly by the kidneys and after intravenous application it is unevenly distributed in the body of mice, the highest concentrations being found in liver and spleen. Daily injections (7.5 mg/kg/day) of entrapped Methotrexate for 5 days into the tail vein of Ehrlich ascites tumor bearing mice reduced both tumor cell count and the production of ascites fluid about fourfold as compared to mice receiving the same dose of Methotrexate in the free form. Six hours after intravenous application of liposome entrapped Methotrexate the tissue concentration in normal liver is ca. 20-fold higher than when the same amount is applied in the free state. On the other hand, only a little uptake of liposome entrapped Methotrexate was detected in the tissue of nitrosamine-induced primary liver tumor when compared to normal liver tissue of rats."}
{"id": "PMID:468897", "title": "Age-dependent change of the effect of a cytostatic drug on the proliferation kinetics of a solid tumor of the mouse.", "content": "The aim of the present investigations was to study the effectiveness of a cytostatic drug (VCR) during different phases of tumor growth (1st, 7th, 14th days a.t.) and at the periphery and at the centre of the tumor (on the 14th day a.t.). Furthermore the inducement of a partial synchronization in the proliferation of tumor cells was attempted. It was found that the intensity of the cytostatic effect significantly decreased both with the passage of time after transplantation and within the tumor from the periphery to the centre. The changing cytostatic sensitivity probably is due to the diminishing vascular supply and the decreased rate of cell proliferation; especially the decline of the growth fraction. A partial synchronization of the proliferation of tumor cells could not be demonstrated.", "contents": "Age-dependent change of the effect of a cytostatic drug on the proliferation kinetics of a solid tumor of the mouse. The aim of the present investigations was to study the effectiveness of a cytostatic drug (VCR) during different phases of tumor growth (1st, 7th, 14th days a.t.) and at the periphery and at the centre of the tumor (on the 14th day a.t.). Furthermore the inducement of a partial synchronization in the proliferation of tumor cells was attempted. It was found that the intensity of the cytostatic effect significantly decreased both with the passage of time after transplantation and within the tumor from the periphery to the centre. The changing cytostatic sensitivity probably is due to the diminishing vascular supply and the decreased rate of cell proliferation; especially the decline of the growth fraction. A partial synchronization of the proliferation of tumor cells could not be demonstrated."}
{"id": "PMID:468898", "title": "Brain tumor chemotherapy using a rat glioma model.", "content": "The antitumor agent chloroethylcyclohexyl-nitrosourea (CCNU) was examined using a rat glioma model. A i.p. administration of three times 40 mg/kg CCNU was highly effective and increased the life-span of tumor-bearing rats from 53 to 86%. A total quantity of CCNU amounting to less than 30 mg/kg was not effective. On the other hand, large dosages exceeding LD10 proved to be toxic. The depression of platelets and white blood cells was mild after a single dosage of 40 mg/kg CCNU which recovered on about the 6th day. In the CCNU-treated animals there was an increase of the extent of necrobiosis. Ballooning of tumor cells with nuclear pyknoses and a lack of mitotic features occurred. Microcystic changes appeared sometimes to be more frequent than in control groups.", "contents": "Brain tumor chemotherapy using a rat glioma model. The antitumor agent chloroethylcyclohexyl-nitrosourea (CCNU) was examined using a rat glioma model. A i.p. administration of three times 40 mg/kg CCNU was highly effective and increased the life-span of tumor-bearing rats from 53 to 86%. A total quantity of CCNU amounting to less than 30 mg/kg was not effective. On the other hand, large dosages exceeding LD10 proved to be toxic. The depression of platelets and white blood cells was mild after a single dosage of 40 mg/kg CCNU which recovered on about the 6th day. In the CCNU-treated animals there was an increase of the extent of necrobiosis. Ballooning of tumor cells with nuclear pyknoses and a lack of mitotic features occurred. Microcystic changes appeared sometimes to be more frequent than in control groups."}
{"id": "PMID:468899", "title": "Influences of inducers and inhibitors of the microsomal monooxygenase system on the alkylating intensity of dimethylnitrosamine in mice.", "content": "Male mice were pretreated with various drugs, which are known either induce or to inhibit cytochrome P 450 function. Five hours after the administration of 14C-dimethylnitrosamine (10 mg/kg) in pretreated and control mice, total and specific radioactivities of cellular macromolecules of the liver were determined. Various types of nucleic acids were extracted and hydrolyzed, and the bases and nucleotides separated by ion-exchange-chromatography. Radioactivity resulting from incorporation and alkylation was measured. Pretreatment of mice with the inducers phenobarbital and 3-Methylcholanthrene decreased the alkylation rates, while administration of the inhibitors SKF 525 A and CFT 1201 caused an increase. These results appear to contradict the accepted activation mechanism of nitrosamines if DMN demethylation as catalyzed by cyt. P 450 is influenced in the same way as other cyt. P 450 dependent reactions.", "contents": "Influences of inducers and inhibitors of the microsomal monooxygenase system on the alkylating intensity of dimethylnitrosamine in mice. Male mice were pretreated with various drugs, which are known either induce or to inhibit cytochrome P 450 function. Five hours after the administration of 14C-dimethylnitrosamine (10 mg/kg) in pretreated and control mice, total and specific radioactivities of cellular macromolecules of the liver were determined. Various types of nucleic acids were extracted and hydrolyzed, and the bases and nucleotides separated by ion-exchange-chromatography. Radioactivity resulting from incorporation and alkylation was measured. Pretreatment of mice with the inducers phenobarbital and 3-Methylcholanthrene decreased the alkylation rates, while administration of the inhibitors SKF 525 A and CFT 1201 caused an increase. These results appear to contradict the accepted activation mechanism of nitrosamines if DMN demethylation as catalyzed by cyt. P 450 is influenced in the same way as other cyt. P 450 dependent reactions."}
{"id": "PMID:468900", "title": "Antitumor activity of 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) and 1-(2-chloroethyl)-3-(4-methyl-cyclohexyl)-1-nitrosourea (MeCCNU) on Yoshida sarcoma ascites cells implanted into the colon wall of rats.", "content": "Three thousand Yoshida sarcoma cells were inoculated into the wall of the descending colon of each of 120 male Sprague-Dawley rats. On day 8 after the tumor implantation, the animals were at random divided into four groups of 30 rats each. The effect of cyclophosphamide (70 mg/kg), BCNU (25 mg/kg), and methyl-CCNU (45 mg/kg) after single i.p. application was investigated. The Yoshida sarcoma transplanted into the colon is sensitive to all three chemotherapeutic drugs. At the doses given cyclophosphamide showed the best results. The two nitrosoureas had a comparable antitumor activity but methyl-CCNU showed a more distinct toxic effect. The introduction of this model for testing new cytostatics in animal experiments is discussed.", "contents": "Antitumor activity of 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) and 1-(2-chloroethyl)-3-(4-methyl-cyclohexyl)-1-nitrosourea (MeCCNU) on Yoshida sarcoma ascites cells implanted into the colon wall of rats. Three thousand Yoshida sarcoma cells were inoculated into the wall of the descending colon of each of 120 male Sprague-Dawley rats. On day 8 after the tumor implantation, the animals were at random divided into four groups of 30 rats each. The effect of cyclophosphamide (70 mg/kg), BCNU (25 mg/kg), and methyl-CCNU (45 mg/kg) after single i.p. application was investigated. The Yoshida sarcoma transplanted into the colon is sensitive to all three chemotherapeutic drugs. At the doses given cyclophosphamide showed the best results. The two nitrosoureas had a comparable antitumor activity but methyl-CCNU showed a more distinct toxic effect. The introduction of this model for testing new cytostatics in animal experiments is discussed."}
{"id": "PMID:468901", "title": "DNA damage and repair induced by diazoacetyl derivatives of amino acids with different mechanism of cytotoxicity. Correlations with mutagenicity and carcinogenicity.", "content": "Eight synthetic N-diazoacetyl amino acids, prepared by inserting a diazoacetyl group onto the alpha-nitrogen of a natural amino acid, and two natural diazoazetyl amino acids, azaserine (9-diazoacetyl-L-serine) and DON (6-diazo-5-oxo-L-norleucine), have been studied by autoradiography for their capacity to induce DNA repair synthesis in mouse cells cultivated \"in vitro\". Dose-dependent unscheduled DNA synthesis was present in cells treated with the eight N-diazoacetyl derivatives, and was absent in cells exposed to approximately equitoxic concentrations of azaserine and DON. Azaserine and DON, unlike N-diazoacetyl derivatives, did not alkylate gamma-(4-nitrobenzyl) pyridine at an appreciable extent. When DNA damage (single stranded breaks or weak points in alkali) was measured by the sensitive technique of alkaline elution, DGA was found about 4 times as potent as azaserine and about 12 times as DON on a molar basis, but about 800 and 17,000 times as potent as azaserine and DON respectively by extrapolating to equitoxic concentrations. Carcinogenicity and mutagenicity seem to follow mainly the capability of inducing DNA damage.", "contents": "DNA damage and repair induced by diazoacetyl derivatives of amino acids with different mechanism of cytotoxicity. Correlations with mutagenicity and carcinogenicity. Eight synthetic N-diazoacetyl amino acids, prepared by inserting a diazoacetyl group onto the alpha-nitrogen of a natural amino acid, and two natural diazoazetyl amino acids, azaserine (9-diazoacetyl-L-serine) and DON (6-diazo-5-oxo-L-norleucine), have been studied by autoradiography for their capacity to induce DNA repair synthesis in mouse cells cultivated \"in vitro\". Dose-dependent unscheduled DNA synthesis was present in cells treated with the eight N-diazoacetyl derivatives, and was absent in cells exposed to approximately equitoxic concentrations of azaserine and DON. Azaserine and DON, unlike N-diazoacetyl derivatives, did not alkylate gamma-(4-nitrobenzyl) pyridine at an appreciable extent. When DNA damage (single stranded breaks or weak points in alkali) was measured by the sensitive technique of alkaline elution, DGA was found about 4 times as potent as azaserine and about 12 times as DON on a molar basis, but about 800 and 17,000 times as potent as azaserine and DON respectively by extrapolating to equitoxic concentrations. Carcinogenicity and mutagenicity seem to follow mainly the capability of inducing DNA damage."}
{"id": "PMID:468902", "title": "Use of radioactive gold in the treatment of pleural effusions caused by metastatic cancer.", "content": "This paper presents a series of 12 patients suffering from advanced gynecological cancer with the occurrence of pleural effusions. All patients received at least one intrapleural dose of radioactive gold (100 m Ci Au198) when the necessity for thoracentesis became more than once every three weeks. Three showed no recurrence of fluid, five a significant slowing down of effusion, and four did not show any response. Severe complications were minimal, and were comparable to those resulting from thoracentesis in general.", "contents": "Use of radioactive gold in the treatment of pleural effusions caused by metastatic cancer. This paper presents a series of 12 patients suffering from advanced gynecological cancer with the occurrence of pleural effusions. All patients received at least one intrapleural dose of radioactive gold (100 m Ci Au198) when the necessity for thoracentesis became more than once every three weeks. Three showed no recurrence of fluid, five a significant slowing down of effusion, and four did not show any response. Severe complications were minimal, and were comparable to those resulting from thoracentesis in general."}
{"id": "PMID:468903", "title": "Methylcholanthrene-induced tumors in resistant and sensitive Jensen sarcoma rats. i. effect of fresh prepared MC emulsion.", "content": "By two subcutaneous or intramuscular injections of 20-methylcholanthrene (MC), 5 mg/animal in vegetal oil, followed after 74 days by 10 mg of MC emulsion, sarcomata were obtained in 63.6% of Jensen sarcoma-resistant rats (R) and in 36.8% of Jensen sarcoma-sensitive rats (S). One of the induces sarcomas, transplanted from R to R and then to S rats, presented a very high antigenicity: it strongly immunised receptive rats against the induced sarcoma and also, in a lower degree, against the Jensen sarcoma. Antigenicity was maintained up to the tenth passage.", "contents": "Methylcholanthrene-induced tumors in resistant and sensitive Jensen sarcoma rats. i. effect of fresh prepared MC emulsion. By two subcutaneous or intramuscular injections of 20-methylcholanthrene (MC), 5 mg/animal in vegetal oil, followed after 74 days by 10 mg of MC emulsion, sarcomata were obtained in 63.6% of Jensen sarcoma-resistant rats (R) and in 36.8% of Jensen sarcoma-sensitive rats (S). One of the induces sarcomas, transplanted from R to R and then to S rats, presented a very high antigenicity: it strongly immunised receptive rats against the induced sarcoma and also, in a lower degree, against the Jensen sarcoma. Antigenicity was maintained up to the tenth passage."}
{"id": "PMID:468904", "title": "Carcinogenicity of chlorinated nitrosotrialkylureas in rats.", "content": "Four chlorinated derivatives of nitrosotrialkylureas were tested for carcinogenicity by oral administration by gavage to Sprague-Dawley rats. All of the compounds were very toxic, and all were carcinogenic, inducing tumors of the non-glandular stomach and of the lungs. The commonest tumors were carcinomas and papillomas of the non-glandular stomach and alveolar cell adenomas of the lungs. Nitrosochloroethyldimethylurea induced most tumors, but it was possible to administer higher doses of this compound than of the others, which were nitrosochloroethydiethylurea, nitrosomethylbis-(chloroethyl)-urea and nitrosotris-(chloroethyl)-urea. The last of these was a particularly toxic compound so that the maximum total dose given was only 0.07 millimole per rat.", "contents": "Carcinogenicity of chlorinated nitrosotrialkylureas in rats. Four chlorinated derivatives of nitrosotrialkylureas were tested for carcinogenicity by oral administration by gavage to Sprague-Dawley rats. All of the compounds were very toxic, and all were carcinogenic, inducing tumors of the non-glandular stomach and of the lungs. The commonest tumors were carcinomas and papillomas of the non-glandular stomach and alveolar cell adenomas of the lungs. Nitrosochloroethyldimethylurea induced most tumors, but it was possible to administer higher doses of this compound than of the others, which were nitrosochloroethydiethylurea, nitrosomethylbis-(chloroethyl)-urea and nitrosotris-(chloroethyl)-urea. The last of these was a particularly toxic compound so that the maximum total dose given was only 0.07 millimole per rat."}
{"id": "PMID:468905", "title": "Ascites tumors in CBA mice. Characterization of two new tumors, a carcinoma and a sarcoma in solid and ascites form, with regard to cell surface properties and transplantability.", "content": "Two ascites tumors in syngeneic CBA mice are described, viz., MCB 21-AA and MCB 31-AA, with their solid progenitors: A sarcoma (MCB 21-SS) and a squamous cell carcinoma (MCB 31-SC), induced by gastric feeding of 20-methylcholanthrene. The ascites tumor cells have certain characteristics in common, which they do not share with either cells from the solid tumors or even with cells from solid ascites tumors (-21-AS and -31-AS=ascites tumor transplanted s.c.). Presumably some of these differences, for instance, in PAS stainability, electrophoretic mobility and lectin agglutinability, are due to enzyme treatment required to bring solid tumors into suspension. Between the two ascites tumors there are certain differences in cell size, aggregability, and growth rate. They are similar, however, in requiring large cell doses for transplantation in syngeneic animals, which is also true for the solid  (SS and SC) tumors. MCB 21 and -AA even required fewer cells for transplantation in allogeneic A mice than in syngeneic CBA mice. MCB 31-AA is also allotransplantable. The pattern of spread, after i.v. cell injection, is almost exclusively to the lungs for all tumor lines.", "contents": "Ascites tumors in CBA mice. Characterization of two new tumors, a carcinoma and a sarcoma in solid and ascites form, with regard to cell surface properties and transplantability. Two ascites tumors in syngeneic CBA mice are described, viz., MCB 21-AA and MCB 31-AA, with their solid progenitors: A sarcoma (MCB 21-SS) and a squamous cell carcinoma (MCB 31-SC), induced by gastric feeding of 20-methylcholanthrene. The ascites tumor cells have certain characteristics in common, which they do not share with either cells from the solid tumors or even with cells from solid ascites tumors (-21-AS and -31-AS=ascites tumor transplanted s.c.). Presumably some of these differences, for instance, in PAS stainability, electrophoretic mobility and lectin agglutinability, are due to enzyme treatment required to bring solid tumors into suspension. Between the two ascites tumors there are certain differences in cell size, aggregability, and growth rate. They are similar, however, in requiring large cell doses for transplantation in syngeneic animals, which is also true for the solid  (SS and SC) tumors. MCB 21 and -AA even required fewer cells for transplantation in allogeneic A mice than in syngeneic CBA mice. MCB 31-AA is also allotransplantable. The pattern of spread, after i.v. cell injection, is almost exclusively to the lungs for all tumor lines."}
{"id": "PMID:468906", "title": "Intestinal metaplasia and adenocarcinoma induced in the stomach of rats by N-propyl-N'-nitro-N-nitrosoguanidine.", "content": "PNNG, the propyl derivative of MNNG, was administered to Wistar rats by a concentration of 59.5 micrograms/ml in the drinking water for 4, 8, and 12 months and the rats were killed in the 15th month. Intestinal metaplasia was induced in the glandular stomachs of 25%, 75%, and 83% of the rats treated with PNNG for 4, 8, and 12 months, respectively. Metaplastic glands were found in the pyloric region, especially near the pyloric ring. These glands contained goblet cells and columnar cells with striated borders. No tumors were found in the stomach of rats after 4-months treatment, but adenomas were found after 8-months treatment, and both adenomas and adenocarcinomas after 12-months treatment.", "contents": "Intestinal metaplasia and adenocarcinoma induced in the stomach of rats by N-propyl-N'-nitro-N-nitrosoguanidine. PNNG, the propyl derivative of MNNG, was administered to Wistar rats by a concentration of 59.5 micrograms/ml in the drinking water for 4, 8, and 12 months and the rats were killed in the 15th month. Intestinal metaplasia was induced in the glandular stomachs of 25%, 75%, and 83% of the rats treated with PNNG for 4, 8, and 12 months, respectively. Metaplastic glands were found in the pyloric region, especially near the pyloric ring. These glands contained goblet cells and columnar cells with striated borders. No tumors were found in the stomach of rats after 4-months treatment, but adenomas were found after 8-months treatment, and both adenomas and adenocarcinomas after 12-months treatment."}
{"id": "PMID:468907", "title": "Electron probe analysis of vascular smooth muscle. Composition of mitochondria, nuclei, and cytoplasm.", "content": "Electron probe analysis of dry cryosections was used to determine the composition of the cytoplasm and organelles of rabbit portal-anterior mesenteric vein (PAMV) smooth muscle. All analytical values given are in mmol/kg wt +/- SEM. Cytoplasmic concentrations in normal, resting muscles were: K, 611 +/- 1.7; Na, 167 +/- 2.7; Cl, 278 +/- 1.0; Mg, 36 +/- 1.1; Ca, 1.9 +/- 0.5; and P, 247 +/- 1.1. Hence, the sum of intracellular Na + K exceeded cytoplasmic Cl by 500 mmol/kg dry wt, while the calculated total, nondiffusible solute was approximately 50 mmol/kg. Cytoplasmic K and Cl were increased in smooth muscles incubated in solutions containing an excess (80 mM) of KCl. Nuclear and cytoplasmic Na and Ca concentrations were not significantly different. The mitochondrial Ca content in normal fibers was low, 0.8 +/- 0.5, and there was no evidence of mitochondrial Ca sequestration in muscles frozen after a K contracture lasint 30 min. Transmitochondrial gradients of K, Na, and Cl were small (0.9--1.2). In damaged fibers, massive mitochondrial Ca accumulation of up to 2 mol/kg dry wt in granule form and associated with P could be demonstrated. Our findings suggest (a) that the nonDonnan distribution of Cl in smooth muscle is not caused by sequestration in organelles, and that considerations of osmotic equilibrium and electroneutrality suggest the existence of unidentified nondiffusible anions in smooth muscle, (b) that nuclei do not contain concentrations of Na or Ca in excess of cytoplasmic levels, (c) that mitochondria in PAMV smooth muscle do not play a major role in regulating cytoplasmic Ca during physiological levels of contraction but can be massively Ca loaded in damaged cells, and (d) that the in situ transmitochondrial gradients of K, Na, and Cl do not show these ions to be distributed according to a large electromotive Donnan force.", "contents": "Electron probe analysis of vascular smooth muscle. Composition of mitochondria, nuclei, and cytoplasm. Electron probe analysis of dry cryosections was used to determine the composition of the cytoplasm and organelles of rabbit portal-anterior mesenteric vein (PAMV) smooth muscle. All analytical values given are in mmol/kg wt +/- SEM. Cytoplasmic concentrations in normal, resting muscles were: K, 611 +/- 1.7; Na, 167 +/- 2.7; Cl, 278 +/- 1.0; Mg, 36 +/- 1.1; Ca, 1.9 +/- 0.5; and P, 247 +/- 1.1. Hence, the sum of intracellular Na + K exceeded cytoplasmic Cl by 500 mmol/kg dry wt, while the calculated total, nondiffusible solute was approximately 50 mmol/kg. Cytoplasmic K and Cl were increased in smooth muscles incubated in solutions containing an excess (80 mM) of KCl. Nuclear and cytoplasmic Na and Ca concentrations were not significantly different. The mitochondrial Ca content in normal fibers was low, 0.8 +/- 0.5, and there was no evidence of mitochondrial Ca sequestration in muscles frozen after a K contracture lasint 30 min. Transmitochondrial gradients of K, Na, and Cl were small (0.9--1.2). In damaged fibers, massive mitochondrial Ca accumulation of up to 2 mol/kg dry wt in granule form and associated with P could be demonstrated. Our findings suggest (a) that the nonDonnan distribution of Cl in smooth muscle is not caused by sequestration in organelles, and that considerations of osmotic equilibrium and electroneutrality suggest the existence of unidentified nondiffusible anions in smooth muscle, (b) that nuclei do not contain concentrations of Na or Ca in excess of cytoplasmic levels, (c) that mitochondria in PAMV smooth muscle do not play a major role in regulating cytoplasmic Ca during physiological levels of contraction but can be massively Ca loaded in damaged cells, and (d) that the in situ transmitochondrial gradients of K, Na, and Cl do not show these ions to be distributed according to a large electromotive Donnan force."}
{"id": "PMID:468908", "title": "Cytochalasin B inhibition of endothelial proliferation at wound edges in vitro.", "content": "Cytochalasin B prevents both migration and wound-associated replication when applied to wounded monolayers of bovine endothelium in tissue culture. The normal low background rate of replication in undisturbed areas of the cultures is not inhibited by cytochalasin B. These results suggest that some form of movement may be required for initiation of wound-associated replication by endothelial cells.", "contents": "Cytochalasin B inhibition of endothelial proliferation at wound edges in vitro. Cytochalasin B prevents both migration and wound-associated replication when applied to wounded monolayers of bovine endothelium in tissue culture. The normal low background rate of replication in undisturbed areas of the cultures is not inhibited by cytochalasin B. These results suggest that some form of movement may be required for initiation of wound-associated replication by endothelial cells."}
{"id": "PMID:468909", "title": "Sodium uptake and membrane excitation in Paramecium.", "content": "Although the phenotypes of many membrane-excitation mutants of Paramecium are best expressed in Na+-containing solutions, little is known about the role of Na+ in membrane excitation in Paramecium. By measuring 22Na fluxes, we have shown that: (a) The total cellular Na+ content is equivalent to a cytoplasmic concentration of 3--4 mM, if the Na+ concentration is uniform throughout the cell. (b) The kinetics of Na+ uptake can be divided into a saturable Na+ uptake with an apparent Km = 0.15 mM and a nonsaturable Na+ uptake seen at higher Na+ concentrations up to 20 mM. (c) The rate of Na+ uptake in high Na+ solutions is correlated with the duration of backward swimming and membrane excitation in wild type Paramecium and the mutants fast-2 and paranoiac. (d) Na+ uptake is inhibited at 4 degrees C. From these results, we postulate that Na+ uptake is faster when the membrane is depolarized than when it is at the resting potential level.", "contents": "Sodium uptake and membrane excitation in Paramecium. Although the phenotypes of many membrane-excitation mutants of Paramecium are best expressed in Na+-containing solutions, little is known about the role of Na+ in membrane excitation in Paramecium. By measuring 22Na fluxes, we have shown that: (a) The total cellular Na+ content is equivalent to a cytoplasmic concentration of 3--4 mM, if the Na+ concentration is uniform throughout the cell. (b) The kinetics of Na+ uptake can be divided into a saturable Na+ uptake with an apparent Km = 0.15 mM and a nonsaturable Na+ uptake seen at higher Na+ concentrations up to 20 mM. (c) The rate of Na+ uptake in high Na+ solutions is correlated with the duration of backward swimming and membrane excitation in wild type Paramecium and the mutants fast-2 and paranoiac. (d) Na+ uptake is inhibited at 4 degrees C. From these results, we postulate that Na+ uptake is faster when the membrane is depolarized than when it is at the resting potential level."}
{"id": "PMID:468910", "title": "Spermatogenesis in the mouse. I. Autoradiographic studies of nuclear incorporation and loss of 3H-amino acids.", "content": "Autoradiographic and electron microscope methods were used to correlate changes in nucleoproteins with nuclear fine structure during spermatogenesis in the mouse. Testes were fixed at daily intervals after intratesticular injectionwith labeled amino acid. [3H]Arginine, lysine, valine, and proline were rapidly incorporated into primary spermatocyte nuclei, retained through subsequent spermatocyte divisions and through spermatid differentiation to step 12 of spermiogenesis, but were lost with spermatid differentiation beyond step 12. Arginine and lysine (not valine or proline) also were rapidly incorporated into certain elongated spermatid nuclei but differed strikingly in their distribution and fate. Nuclei of late step-12 through step-15 spermatids were initially labeled with arginine. This label was retained through subsequent spermatid differentiation and sperm maturation in the epididymis. By contrast, lysine was initially incorporated only into late step-12 and step-13 spermatid nuclei, and was retained only to early step 14 of spermiogenesis. Spermatid incorporation of lysine coincided with the initiation of chromatin condensation in late step-12 nuclei, and loss of lysine coincided with the completion of condensation in step-14 nuclei.", "contents": "Spermatogenesis in the mouse. I. Autoradiographic studies of nuclear incorporation and loss of 3H-amino acids. Autoradiographic and electron microscope methods were used to correlate changes in nucleoproteins with nuclear fine structure during spermatogenesis in the mouse. Testes were fixed at daily intervals after intratesticular injectionwith labeled amino acid. [3H]Arginine, lysine, valine, and proline were rapidly incorporated into primary spermatocyte nuclei, retained through subsequent spermatocyte divisions and through spermatid differentiation to step 12 of spermiogenesis, but were lost with spermatid differentiation beyond step 12. Arginine and lysine (not valine or proline) also were rapidly incorporated into certain elongated spermatid nuclei but differed strikingly in their distribution and fate. Nuclei of late step-12 through step-15 spermatids were initially labeled with arginine. This label was retained through subsequent spermatid differentiation and sperm maturation in the epididymis. By contrast, lysine was initially incorporated only into late step-12 and step-13 spermatid nuclei, and was retained only to early step 14 of spermiogenesis. Spermatid incorporation of lysine coincided with the initiation of chromatin condensation in late step-12 nuclei, and loss of lysine coincided with the completion of condensation in step-14 nuclei."}
{"id": "PMID:468911", "title": "Membrane events involved in myoblast fusion.", "content": "Myoblast fusion has been studied in cultures of chick embryonic muscle utilizing ultrastructural techniques. The multinucleated muscle cells (myotubes) are generated by the fusion of two plasma membranes from adjacent cells, apparently by forming a single bilayer that is particle-free in freeze-fracture replicas. This single bilayer subsequently collapses, and cytoplasmic continuity is established between the cells. The fusion between the two plasma membranes appears to take place primarily within particle-free domains (probably phospholipid enriched), and cytoplasmic unilamellar, particle-free vesicles are occasionally associated with these regions. These vesicles structurally resemble phospholipid vesicles (liposomes). They are present in normal myoblasts, but they are absent in certain fusion-arrested myoblast popluations, such as those treated with either 5-bromo-deoxyuridine (BUdR), cycloheximide (CHX), or pospholipase C (PLC). The unilamellar, particle-free vesicles are present in close proximity to the plasma membranes, and physical contact is observed frequently between the vesicle membrane and the plasma membrane. The regions of vesicle membrane-plasma membrane interaction are characteristically free of intramembrane particles. A model for myoblast fusion is presented that is based onan interpretation of these observations. This model suggests that the cytoplasmic vesicles initiate the generation of particle-depleted membrane domains, both being essential components in the fusion process.", "contents": "Membrane events involved in myoblast fusion. Myoblast fusion has been studied in cultures of chick embryonic muscle utilizing ultrastructural techniques. The multinucleated muscle cells (myotubes) are generated by the fusion of two plasma membranes from adjacent cells, apparently by forming a single bilayer that is particle-free in freeze-fracture replicas. This single bilayer subsequently collapses, and cytoplasmic continuity is established between the cells. The fusion between the two plasma membranes appears to take place primarily within particle-free domains (probably phospholipid enriched), and cytoplasmic unilamellar, particle-free vesicles are occasionally associated with these regions. These vesicles structurally resemble phospholipid vesicles (liposomes). They are present in normal myoblasts, but they are absent in certain fusion-arrested myoblast popluations, such as those treated with either 5-bromo-deoxyuridine (BUdR), cycloheximide (CHX), or pospholipase C (PLC). The unilamellar, particle-free vesicles are present in close proximity to the plasma membranes, and physical contact is observed frequently between the vesicle membrane and the plasma membrane. The regions of vesicle membrane-plasma membrane interaction are characteristically free of intramembrane particles. A model for myoblast fusion is presented that is based onan interpretation of these observations. This model suggests that the cytoplasmic vesicles initiate the generation of particle-depleted membrane domains, both being essential components in the fusion process."}
{"id": "PMID:468912", "title": "Effects of colchicine on endocytosis and cellular inactivation of horseradish peroxidase in cultured chondrocytes.", "content": "Horseradish peroxidase (HRP) was used as a marker to study the effects of microtubule-disruptive drugs on uptake and cellular inactivation of exogenous material in cultures of embryonic chick chondrocytes. HRP was ingested by fluid endocytosis, and intracellular enzyme activity subsequently diminished exponentially with time. Cytochemically, reaction product for HRP was found in vesicles often located close to the dictyosomes of the Golgi complex. Colchicine and vinblastine caused disappearance of cytoplasmic microtubules and disorganization of the Golgi complex with concomitant reduction in the cellular uptake of HRP to about half of that in the controls. Lumicolchicine, on the other hand, left cell fine structure and HRP uptake unaffected. These results indicate that microtubules are of considerable importance in the process of fluid endocytosis in cultured chondrocytes although the exact mechanism remains to be elucidated. The rate of intracellular inactivation of ingested HRP was not affected by colchicine or vinblastine. Double-labeling experiments with colloidal thorium dioxide and HRP likewise indicated that fusion of endocytic vesicles and lysosomes is not dependent on intact microtubules. The total specific activities of the three lysosomal enzymes examined were weakly or not at all changed by treatment of the cultures with colchicine or vinblastine. It therefore seems unlikely that microtubular organization plays an important role in the production or degradation of lysosomal enzymes in cultured chondrocytes.", "contents": "Effects of colchicine on endocytosis and cellular inactivation of horseradish peroxidase in cultured chondrocytes. Horseradish peroxidase (HRP) was used as a marker to study the effects of microtubule-disruptive drugs on uptake and cellular inactivation of exogenous material in cultures of embryonic chick chondrocytes. HRP was ingested by fluid endocytosis, and intracellular enzyme activity subsequently diminished exponentially with time. Cytochemically, reaction product for HRP was found in vesicles often located close to the dictyosomes of the Golgi complex. Colchicine and vinblastine caused disappearance of cytoplasmic microtubules and disorganization of the Golgi complex with concomitant reduction in the cellular uptake of HRP to about half of that in the controls. Lumicolchicine, on the other hand, left cell fine structure and HRP uptake unaffected. These results indicate that microtubules are of considerable importance in the process of fluid endocytosis in cultured chondrocytes although the exact mechanism remains to be elucidated. The rate of intracellular inactivation of ingested HRP was not affected by colchicine or vinblastine. Double-labeling experiments with colloidal thorium dioxide and HRP likewise indicated that fusion of endocytic vesicles and lysosomes is not dependent on intact microtubules. The total specific activities of the three lysosomal enzymes examined were weakly or not at all changed by treatment of the cultures with colchicine or vinblastine. It therefore seems unlikely that microtubular organization plays an important role in the production or degradation of lysosomal enzymes in cultured chondrocytes."}
{"id": "PMID:468913", "title": "Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones.", "content": "Isolated renal cortical collecting tubules obtained from rabbits treated chronically with desoxycorticosterone acetate (DOCA) have been found to possess elevated transepithelial potential differences and a greatly increased capacity for ion transport. Structural exmination of tubules from rabbits exposed to either DOCA or dexamethasone for 11--18 d reveals a marked increase in basolateral cell membrane area in these tubules. Morphometric analysis shows that this effect is specifically on the basolateral membrane area of only one of the two cell types found in this nephron segment. Increases of greater than 140% and 90% are found for the basolateral membrane area of the principal cells for DOCA and dexamethasone, respectively, but no change could be detected in the basolateral membrane area of the intercalated cells found in this nephron segment. No siginificant changes were found in luminal membrane area, cell number, or cell volume for either cell type. These observations demonstrate that significant changes in membrane area can occur in differentiated epithelia and suggest that this may be an important mechanism for modulating epithelial transport capacity.", "contents": "Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones. Isolated renal cortical collecting tubules obtained from rabbits treated chronically with desoxycorticosterone acetate (DOCA) have been found to possess elevated transepithelial potential differences and a greatly increased capacity for ion transport. Structural exmination of tubules from rabbits exposed to either DOCA or dexamethasone for 11--18 d reveals a marked increase in basolateral cell membrane area in these tubules. Morphometric analysis shows that this effect is specifically on the basolateral membrane area of only one of the two cell types found in this nephron segment. Increases of greater than 140% and 90% are found for the basolateral membrane area of the principal cells for DOCA and dexamethasone, respectively, but no change could be detected in the basolateral membrane area of the intercalated cells found in this nephron segment. No siginificant changes were found in luminal membrane area, cell number, or cell volume for either cell type. These observations demonstrate that significant changes in membrane area can occur in differentiated epithelia and suggest that this may be an important mechanism for modulating epithelial transport capacity."}
{"id": "PMID:468914", "title": "Incorporation of membrane proteins into large single bilayer vesicles. Application to rhodopsin.", "content": "A general procedure to incorporate membrane proteins in a native state into large single bilayer vesicles is described. The results obtained with rhodopsin from vertebrate and invertebrate retinas are presented. The technique involves: (a) the direct transfer of rhodopsin-lipid complexes from native membranes into ether or pentane, and (b) the sonication of the complex in apolar solvent with aqueous buffer followed by solvent evaporation under reduced pressure. The spectral properties of rhodopsin in the large vesicles are similar to those of rhodopsin in photoreceptors; furthermore, bleached bovine rhodopsin is chemically regenerable with 9-cis retinal. These results establish the presence of photochemically functional rhodopsin in the large vesicles. Freeze-fracture replicas of the vesicles reveal that both internal and external leaflets contain numerous particles approximately 80 A in diameter, indicating that rhodopsin is symmetrically distributed within the bilayer. More than 75% of the membrane area is incorporated into vesicles larger than 0.5 micron and approximately 40% into vesicles larger than 1 micron.", "contents": "Incorporation of membrane proteins into large single bilayer vesicles. Application to rhodopsin. A general procedure to incorporate membrane proteins in a native state into large single bilayer vesicles is described. The results obtained with rhodopsin from vertebrate and invertebrate retinas are presented. The technique involves: (a) the direct transfer of rhodopsin-lipid complexes from native membranes into ether or pentane, and (b) the sonication of the complex in apolar solvent with aqueous buffer followed by solvent evaporation under reduced pressure. The spectral properties of rhodopsin in the large vesicles are similar to those of rhodopsin in photoreceptors; furthermore, bleached bovine rhodopsin is chemically regenerable with 9-cis retinal. These results establish the presence of photochemically functional rhodopsin in the large vesicles. Freeze-fracture replicas of the vesicles reveal that both internal and external leaflets contain numerous particles approximately 80 A in diameter, indicating that rhodopsin is symmetrically distributed within the bilayer. More than 75% of the membrane area is incorporated into vesicles larger than 0.5 micron and approximately 40% into vesicles larger than 1 micron."}
{"id": "PMID:468915", "title": "Nucleosome packing in interphase chromatin.", "content": "Higher-order chromatin fibers (200--300 A in diameter) are reproducibly released from nuclei after lysis in the absence of formalin and/or detergent. Electron microscope analysis of these fibers shows that they are composed of a continuous array of closely apposed nucleosomes which display several distinct packing patterns. Analysis of the organization of nucleosomes within these arrays and their distribution along long stretches of chromatin suggest that the basic 100-A chromatin fiber is not packed into discrete superbeads and is not folded into a uniform solenoid within the native 250-A fiber. Furthermore, because similar higher-order fibers have been visualized in metaphase chromosomes, the existence of this fiber class appears to be independent of the degree of in vivo chromatin condensation.", "contents": "Nucleosome packing in interphase chromatin. Higher-order chromatin fibers (200--300 A in diameter) are reproducibly released from nuclei after lysis in the absence of formalin and/or detergent. Electron microscope analysis of these fibers shows that they are composed of a continuous array of closely apposed nucleosomes which display several distinct packing patterns. Analysis of the organization of nucleosomes within these arrays and their distribution along long stretches of chromatin suggest that the basic 100-A chromatin fiber is not packed into discrete superbeads and is not folded into a uniform solenoid within the native 250-A fiber. Furthermore, because similar higher-order fibers have been visualized in metaphase chromosomes, the existence of this fiber class appears to be independent of the degree of in vivo chromatin condensation."}
{"id": "PMID:468916", "title": "Enhancement of chemotactic response and microtubule assembly in human leukocytes by ascorbic acid.", "content": "The incubation of human leukocytes with ascorbic acid increased chemotaxis of the cells. In addition, ascorbic acid promoted the assembly of intracellular polymorphonuclear leukocyte (PMN) with colchicine blocked the effect of ascorbic acid on promoting microtubule assembly. Not only did ascorbic acid promote the assembly of microtubules in vivo, but it enhanced the assembly of bovine brain tubulin into microtubules in vitro as quantitated by a glass-fiber filtration assay and by promotion of viscosity changes. The enhancement in leukocyte mobility by ascorbate at concentrations achievable in normal tissues correlates with its ability to assemble microtubule organelles.", "contents": "Enhancement of chemotactic response and microtubule assembly in human leukocytes by ascorbic acid. The incubation of human leukocytes with ascorbic acid increased chemotaxis of the cells. In addition, ascorbic acid promoted the assembly of intracellular polymorphonuclear leukocyte (PMN) with colchicine blocked the effect of ascorbic acid on promoting microtubule assembly. Not only did ascorbic acid promote the assembly of microtubules in vivo, but it enhanced the assembly of bovine brain tubulin into microtubules in vitro as quantitated by a glass-fiber filtration assay and by promotion of viscosity changes. The enhancement in leukocyte mobility by ascorbate at concentrations achievable in normal tissues correlates with its ability to assemble microtubule organelles."}
{"id": "PMID:468917", "title": "Transformed cells have lost control of ribosome number through their growth cycle.", "content": "Previous studies on the synthesis and function of the protein synthetic machinery through the growth cycle of normal cultured hamster embryo fibroblasts (HA) were extended here to a series of four different clonal lines of polyoma virus-transformed HA cells. Under our culture conditions, these transformed cells could enter a stationary phase characterized by no mitotic cells, very low rates of DNA synthesis, and arrest in a post-mitotic pre-DNA synthetic state. Cellular viability was initially high in stationary phase but, unlike normal cells, transformed cells slowly lost viability. The rate of protein synthesis in the stationary phase of the transformed cells fell to 25-30% of the exponential rate. Though this reduction was similar to that seen in normal cells, it was accomplished by different means. The specific reduction in the ribosome complement per cell to values below that of any cycling cell seen in normal cells, was not seen in any of the transformed lines. This observation, which implies a loss of normal control of ribisome synthesis through the growth cycle after transformation, was confirmed in normal Chinese hamster embryo fibroblasts and transformed CHO cell lines. Normal control of ribosome synthesis was restored in L-73 and LR-73, growth control revertants of one of the transformed CHO lines. The transformed lines reduced their protein synthetic rates in stationary phase either by a greater reduction in the proportion of functioning ribosomes than that seen in normal cells or by a decrease in the elongation rate of functioning ribosomes; the latter effect was not seen in the normal cells. A model for growth control of normal cells and its derangement in transformed cells is presented.", "contents": "Transformed cells have lost control of ribosome number through their growth cycle. Previous studies on the synthesis and function of the protein synthetic machinery through the growth cycle of normal cultured hamster embryo fibroblasts (HA) were extended here to a series of four different clonal lines of polyoma virus-transformed HA cells. Under our culture conditions, these transformed cells could enter a stationary phase characterized by no mitotic cells, very low rates of DNA synthesis, and arrest in a post-mitotic pre-DNA synthetic state. Cellular viability was initially high in stationary phase but, unlike normal cells, transformed cells slowly lost viability. The rate of protein synthesis in the stationary phase of the transformed cells fell to 25-30% of the exponential rate. Though this reduction was similar to that seen in normal cells, it was accomplished by different means. The specific reduction in the ribosome complement per cell to values below that of any cycling cell seen in normal cells, was not seen in any of the transformed lines. This observation, which implies a loss of normal control of ribisome synthesis through the growth cycle after transformation, was confirmed in normal Chinese hamster embryo fibroblasts and transformed CHO cell lines. Normal control of ribosome synthesis was restored in L-73 and LR-73, growth control revertants of one of the transformed CHO lines. The transformed lines reduced their protein synthetic rates in stationary phase either by a greater reduction in the proportion of functioning ribosomes than that seen in normal cells or by a decrease in the elongation rate of functioning ribosomes; the latter effect was not seen in the normal cells. A model for growth control of normal cells and its derangement in transformed cells is presented."}
{"id": "PMID:468918", "title": "Enhanced sterol synthesis in concanavalin A-stimulated lymphocytes: correlation with phospholipid synthesis and DNA synthesis.", "content": "Incorporation of (14C)choline and (3H)myo-inositol into the total lipid fraction, incorporation of (14C)acetate into the sterol fraction and incorporation of (3H)thymidine into DNA were studied in human lymphocyte cultures. Concanavalin A induced an increase in the incorporation of these labels with the following features: (a) Phospholipid synthesis was increased promptly. The lag time for the increase in sterol synthesis and DNA synthesis were 5 hours and 27 hours respectively; (b) The increase in phospholipid synthesis and sterol synthesis was proportional to ConA concentration initially. Cells treated with a high concentration of ConA showed very low levels of DNA synthesis; (c) The increase in phospholipid synthesis could be abolished immediately by alpha-Methyl-Mannoside. alpha-Methyl-Mannoside blunted but did not abolish the increase in sterol synthesis. alpha-Methyl-Mannoside enhanced DNA synthesis of those cells which had been treated by a high concentration of ConA; and (d) Selective inhibition of sterol synthesis with 25-hydroxycholesterol did not prevent the increase in phospholipid synthesis, but it blocked the increase in DNA synthesis. Supplement of LDL, HDL or total lipoproteins to lymphocyte cultures was effective in preventing the inhibition of DNA synthesis by 25-hydroxy-cholesterol. These results suggest that in lymphocyte activation by ConA phospholipid synthesis, sterol synthesis and DNA synthesis were sequentially increased. The rate of cellular commitment to mitogenesis was proportional to ConA concentrations. High concentrations of ConA arrested the cell growth at a postcommitment point in the G1 phase. Enhanced phospholipid synthesis was a precommitment event. Enhanced sterol synthesis was a postcommitment event and reflected the requirement of an increased cholesterol supply for the passage of cell growth through G1.", "contents": "Enhanced sterol synthesis in concanavalin A-stimulated lymphocytes: correlation with phospholipid synthesis and DNA synthesis. Incorporation of (14C)choline and (3H)myo-inositol into the total lipid fraction, incorporation of (14C)acetate into the sterol fraction and incorporation of (3H)thymidine into DNA were studied in human lymphocyte cultures. Concanavalin A induced an increase in the incorporation of these labels with the following features: (a) Phospholipid synthesis was increased promptly. The lag time for the increase in sterol synthesis and DNA synthesis were 5 hours and 27 hours respectively; (b) The increase in phospholipid synthesis and sterol synthesis was proportional to ConA concentration initially. Cells treated with a high concentration of ConA showed very low levels of DNA synthesis; (c) The increase in phospholipid synthesis could be abolished immediately by alpha-Methyl-Mannoside. alpha-Methyl-Mannoside blunted but did not abolish the increase in sterol synthesis. alpha-Methyl-Mannoside enhanced DNA synthesis of those cells which had been treated by a high concentration of ConA; and (d) Selective inhibition of sterol synthesis with 25-hydroxycholesterol did not prevent the increase in phospholipid synthesis, but it blocked the increase in DNA synthesis. Supplement of LDL, HDL or total lipoproteins to lymphocyte cultures was effective in preventing the inhibition of DNA synthesis by 25-hydroxy-cholesterol. These results suggest that in lymphocyte activation by ConA phospholipid synthesis, sterol synthesis and DNA synthesis were sequentially increased. The rate of cellular commitment to mitogenesis was proportional to ConA concentrations. High concentrations of ConA arrested the cell growth at a postcommitment point in the G1 phase. Enhanced phospholipid synthesis was a precommitment event. Enhanced sterol synthesis was a postcommitment event and reflected the requirement of an increased cholesterol supply for the passage of cell growth through G1."}
{"id": "PMID:468919", "title": "Stimulation of chondroitin sulfate synthesis by beta-D-xyloside in chondrocytes of the proteoglycan deficient mutant nanomelia.", "content": "The potential of nanomelic chondrocytes to synthesize chondroitin sulfate was investigated by providing the mutant cells with p-nitrophenyl-beta-D-xyloside, a compound which acts as an artificial acceptor for glycosaminoglycan synthesis. Under these conditions the synthesis of chondroitin sulfate in nanomelic and normal chondrocytes is comparable. The chondroitin sulfate synthesized by the mutant is indistinguishable in molecular size and composition from that synthesized by similarly treated normal chondrocytes.", "contents": "Stimulation of chondroitin sulfate synthesis by beta-D-xyloside in chondrocytes of the proteoglycan deficient mutant nanomelia. The potential of nanomelic chondrocytes to synthesize chondroitin sulfate was investigated by providing the mutant cells with p-nitrophenyl-beta-D-xyloside, a compound which acts as an artificial acceptor for glycosaminoglycan synthesis. Under these conditions the synthesis of chondroitin sulfate in nanomelic and normal chondrocytes is comparable. The chondroitin sulfate synthesized by the mutant is indistinguishable in molecular size and composition from that synthesized by similarly treated normal chondrocytes."}
{"id": "PMID:468920", "title": "Characteristics of concanavalin A-resistant Chinese hamster ovary cells and certain revertants.", "content": "Clones of Chinese hamster ovary (CHO) cells were isolated by single-step selection for resistance to killing Concanavalin A (ConA) and certain cellular and membrane properties were examined. The ConA-resistant isolates were only about 2-fold more resistant than wild type cells to the selecting lectin, but exhibited pleiotropic temperature-sensitivity for growth, markedly altered morphology and adherence, and significant difference in susceptibility to other agents such as colchicine. Two revertants to full temperature-resistance were isolated from different ConA-resistant mutants. One revertant clone had reacquired wild type sensitivity to ConA while the other revertant remained ConA-resistant. The two series of wild typed, ConA-resistant, and temperature revertant clones were analyzed for altered mobility of cell surface glycoproteins using lactoperoxidase/125I and galactose oxidase/(3H) borohydride labelling procedures. The ConA-resistant clones showed increased mobility on polyacrylamide gels of three classes of labelled proteins, in the molecular weight ranges 225,000, 200,000, and 130,000 daltons. These changes persisted in the temperature-revertant that remained ConA-resistant, while two of the altered protein closses were restored to wild type mobility in the revertant that regained ConA-sensitivity. Cell hybridization experiments indicated that the temperature-sensitivity phenotypes of different ConA-resistant isolates are recessive and noncomplementing, implying that the same gene is affected in each case. The reversions to temperature resistance appear to be recessive suppressor mutation in different genes.", "contents": "Characteristics of concanavalin A-resistant Chinese hamster ovary cells and certain revertants. Clones of Chinese hamster ovary (CHO) cells were isolated by single-step selection for resistance to killing Concanavalin A (ConA) and certain cellular and membrane properties were examined. The ConA-resistant isolates were only about 2-fold more resistant than wild type cells to the selecting lectin, but exhibited pleiotropic temperature-sensitivity for growth, markedly altered morphology and adherence, and significant difference in susceptibility to other agents such as colchicine. Two revertants to full temperature-resistance were isolated from different ConA-resistant mutants. One revertant clone had reacquired wild type sensitivity to ConA while the other revertant remained ConA-resistant. The two series of wild typed, ConA-resistant, and temperature revertant clones were analyzed for altered mobility of cell surface glycoproteins using lactoperoxidase/125I and galactose oxidase/(3H) borohydride labelling procedures. The ConA-resistant clones showed increased mobility on polyacrylamide gels of three classes of labelled proteins, in the molecular weight ranges 225,000, 200,000, and 130,000 daltons. These changes persisted in the temperature-revertant that remained ConA-resistant, while two of the altered protein closses were restored to wild type mobility in the revertant that regained ConA-sensitivity. Cell hybridization experiments indicated that the temperature-sensitivity phenotypes of different ConA-resistant isolates are recessive and noncomplementing, implying that the same gene is affected in each case. The reversions to temperature resistance appear to be recessive suppressor mutation in different genes."}
{"id": "PMID:468921", "title": "Erythropoietin responses and physical characterization of erythroid progenitor cells in Rauscher virus infected BALB/c mice.", "content": "Infection of BALB/c mice with Rauscher leukemia virus (RLV) gives rise to pronounced erythrocytopoiesis manifesting in splenomegaly and is associated with progressive development of anemia. In the spleen erythroid colony forming units (CFU-E) increase exponentially up to 800-fold that of normal levels by the third week of infection. In vitro these CFU-E are dependent on erythropoietin for colony formation, their erythropoietin requirements being higher than that of CFU-E from normal mice. Numbers of CFU-E in spleen and degree of splenomegaly in anemic RLV infected mice were also shown to be modified by red blood cell transfusion, but progression of the disease was not stopped. Erythroid burst forming units (BFU-E) were also responsive to erythropoietin. However, a small proportion of cells also formed BFU-E colonies at concentrations which did not support growth of normal marrow BFU-E. When compared to normal, CFU-E found in RLV-infected spleen have similar velocity sedimentation rates. However, buoyant density separation of leukemic spleen cells indicated that CFU-E were more homogeneous (modal density 1.0695 g/cm3) than CFU-E from normal spleen. Analysis of physical properties of CFU-E and the nonhemoglobinized erythroblast-like cells, which accumulate in the spleen showed that they differed mainly in their distribution of cell diameter. Our findings show that erythroid progenitor cells in RLV infected mice are responsive to erythropoietin in vitro. Also in vivo erythropoiesis appears to be under control of erythropoietin but other factors which lead to progression of RLV disease apparently exist. Most proerythroblast-like cells, which are characteristic of this disease, apparently lack the potential to form colonies and may be more mature than CFU-E.", "contents": "Erythropoietin responses and physical characterization of erythroid progenitor cells in Rauscher virus infected BALB/c mice. Infection of BALB/c mice with Rauscher leukemia virus (RLV) gives rise to pronounced erythrocytopoiesis manifesting in splenomegaly and is associated with progressive development of anemia. In the spleen erythroid colony forming units (CFU-E) increase exponentially up to 800-fold that of normal levels by the third week of infection. In vitro these CFU-E are dependent on erythropoietin for colony formation, their erythropoietin requirements being higher than that of CFU-E from normal mice. Numbers of CFU-E in spleen and degree of splenomegaly in anemic RLV infected mice were also shown to be modified by red blood cell transfusion, but progression of the disease was not stopped. Erythroid burst forming units (BFU-E) were also responsive to erythropoietin. However, a small proportion of cells also formed BFU-E colonies at concentrations which did not support growth of normal marrow BFU-E. When compared to normal, CFU-E found in RLV-infected spleen have similar velocity sedimentation rates. However, buoyant density separation of leukemic spleen cells indicated that CFU-E were more homogeneous (modal density 1.0695 g/cm3) than CFU-E from normal spleen. Analysis of physical properties of CFU-E and the nonhemoglobinized erythroblast-like cells, which accumulate in the spleen showed that they differed mainly in their distribution of cell diameter. Our findings show that erythroid progenitor cells in RLV infected mice are responsive to erythropoietin in vitro. Also in vivo erythropoiesis appears to be under control of erythropoietin but other factors which lead to progression of RLV disease apparently exist. Most proerythroblast-like cells, which are characteristic of this disease, apparently lack the potential to form colonies and may be more mature than CFU-E."}
{"id": "PMID:468922", "title": "Bromodeoxyuridine-induced reversion of transformed characteristics in BHK21 cells: changes at the plasma membrane level.", "content": "When BHK21 cells transformed by hamster sarcoma virus are grown in the presence of 5-Bromedeoxyuridine (BUdr), several in vitro properties of the transformed cells such as morphology, adhesiveness, and alignment, revert towards a state close to that of untransformed cells. We have studied plasma membrane changes associated with this phenotypic reversion by several different biochemical methods. Reversion is accompanied by a reappearance of Fibronectin, an increase in a membrane-associated protein of M.W. 100,000 which is increased in transformed cells and a decrease in Con A-agglutinability. On the other hand, several membrane changes associated with malignant transformation namely, the increase in an integral membrane protein M.W. 177,000, the higher rate of hexose uptake, the increase in high molecular weight surface glycopeptides and, to some extent, the increase in the density of intramembranous particles, did not revert under BUdr treatment. Thus, membrane properties of transformed cells may be dissociated into two main groups by BUdr treatment. In addition, the exposure and glycosylation of a growth-regulated membrane protein M.W. 160,000 was highly sensitive to BUdr.", "contents": "Bromodeoxyuridine-induced reversion of transformed characteristics in BHK21 cells: changes at the plasma membrane level. When BHK21 cells transformed by hamster sarcoma virus are grown in the presence of 5-Bromedeoxyuridine (BUdr), several in vitro properties of the transformed cells such as morphology, adhesiveness, and alignment, revert towards a state close to that of untransformed cells. We have studied plasma membrane changes associated with this phenotypic reversion by several different biochemical methods. Reversion is accompanied by a reappearance of Fibronectin, an increase in a membrane-associated protein of M.W. 100,000 which is increased in transformed cells and a decrease in Con A-agglutinability. On the other hand, several membrane changes associated with malignant transformation namely, the increase in an integral membrane protein M.W. 177,000, the higher rate of hexose uptake, the increase in high molecular weight surface glycopeptides and, to some extent, the increase in the density of intramembranous particles, did not revert under BUdr treatment. Thus, membrane properties of transformed cells may be dissociated into two main groups by BUdr treatment. In addition, the exposure and glycosylation of a growth-regulated membrane protein M.W. 160,000 was highly sensitive to BUdr."}
{"id": "PMID:468923", "title": "A problem-oriented analysis of database models.", "content": "Which is the most convenient database model considering specific applications? The goal of this paper is to try to answer this question by the use of a chemical example. Examples of requests describe the problems of insertion, deletion, and updating; these requests are analyzed for the hierarchical model and are expressed in a relational language defined by the authors and in Socrate for the network model.", "contents": "A problem-oriented analysis of database models. Which is the most convenient database model considering specific applications? The goal of this paper is to try to answer this question by the use of a chemical example. Examples of requests describe the problems of insertion, deletion, and updating; these requests are analyzed for the hierarchical model and are expressed in a relational language defined by the authors and in Socrate for the network model."}
{"id": "PMID:468924", "title": "[Sarcoma of the body of the uterus. Report of 8 cases (author's transl)].", "content": "The authors report 8 cases of sarcoma of the uterus and discuss the special characteristics of this type of lesion. It seems that the frequency of sarcoma of the uterus is more importnat than is usually thought : one case out of 100 fibromas in the authors' series. The pre-operative diagnosis is difficult, in particular the radiological signs described by Musset were only found in 2 cases out of 8. On the other hand, the pre-operative diagnosis was made in 5 cases out of 8. The histological diagnosis is difficult and may only be made later. The only treatment is surgical, according to most authors. Chemotherapy may be of interest in the future. A uterine sarcoma should certainly be considered more often, especially in cases of fibroma which undergo rapid change.", "contents": "[Sarcoma of the body of the uterus. Report of 8 cases (author's transl)]. The authors report 8 cases of sarcoma of the uterus and discuss the special characteristics of this type of lesion. It seems that the frequency of sarcoma of the uterus is more importnat than is usually thought : one case out of 100 fibromas in the authors' series. The pre-operative diagnosis is difficult, in particular the radiological signs described by Musset were only found in 2 cases out of 8. On the other hand, the pre-operative diagnosis was made in 5 cases out of 8. The histological diagnosis is difficult and may only be made later. The only treatment is surgical, according to most authors. Chemotherapy may be of interest in the future. A uterine sarcoma should certainly be considered more often, especially in cases of fibroma which undergo rapid change."}
{"id": "PMID:468925", "title": "[A case of papillary disinsertion during gastro-duo-denectomy for ulcer of the second part of the duodenum and its repair by a \"physiological\" reconstruction (author's transl)].", "content": "The authors report a personal case of repair of papillary disinsertion. They emphasise the risk of retrograde dissection of an old stenosing duodenal ulcer, the importance of peroperative recognition of this accident by cholangiography, the interest of sphincterotomy to attempt to prevent secondary stenosis of the papilla, the absolute necessity of an anastomosis preventing anastomotic ulcer by contact between the mouth of the gastro-enterostomy and the alkaline secretions. Vagotomy seems to them a useful complementary procedure but, associated with a drainage procedure, they believe it might have avoided this severe complication.", "contents": "[A case of papillary disinsertion during gastro-duo-denectomy for ulcer of the second part of the duodenum and its repair by a \"physiological\" reconstruction (author's transl)]. The authors report a personal case of repair of papillary disinsertion. They emphasise the risk of retrograde dissection of an old stenosing duodenal ulcer, the importance of peroperative recognition of this accident by cholangiography, the interest of sphincterotomy to attempt to prevent secondary stenosis of the papilla, the absolute necessity of an anastomosis preventing anastomotic ulcer by contact between the mouth of the gastro-enterostomy and the alkaline secretions. Vagotomy seems to them a useful complementary procedure but, associated with a drainage procedure, they believe it might have avoided this severe complication."}
{"id": "PMID:468926", "title": "[A case of thymolipoma in a child (author's transl)].", "content": "A new case of asymptomatic thymolipoma in a 4.5 years old girl is reported. It was discovered during systematic medical investigation. Its radiological characteristics and the lack of evolutivity made the authors suspect the diagnosis before operating. A total surgical extraction of the 300 g weighing tumor was performed through a right thoracotomy. No complication occurred in the post-operative time. Others authors already indicated the scarcity of this affection (less than 10 cases in children) as well as the special radiological characteristics of this tumor and the noticeable clinical tolerance often despite a large bulk. Histologically the tumor is made of an infiltrating of the normal thymic tissue by proliferating adipose adulte cells, the benignity of which is demonstrated by the evolution. In this study a differential diagnosis between thymolipoma and other mediastinal tumors is made and also some pathogenic assessments. Surgery always is required for treatment.", "contents": "[A case of thymolipoma in a child (author's transl)]. A new case of asymptomatic thymolipoma in a 4.5 years old girl is reported. It was discovered during systematic medical investigation. Its radiological characteristics and the lack of evolutivity made the authors suspect the diagnosis before operating. A total surgical extraction of the 300 g weighing tumor was performed through a right thoracotomy. No complication occurred in the post-operative time. Others authors already indicated the scarcity of this affection (less than 10 cases in children) as well as the special radiological characteristics of this tumor and the noticeable clinical tolerance often despite a large bulk. Histologically the tumor is made of an infiltrating of the normal thymic tissue by proliferating adipose adulte cells, the benignity of which is demonstrated by the evolution. In this study a differential diagnosis between thymolipoma and other mediastinal tumors is made and also some pathogenic assessments. Surgery always is required for treatment."}
{"id": "PMID:468927", "title": "[Use of electrolyte solutions in surgery (author's transl)].", "content": "The use of isotonic saline and Ringer's lactate in surgery has become widespread following the work of Shires on the sequestration of extra-cellular fluid in hemorrhagic shock. Their use both in military and civilian practice and during hemodilutions in cardiac surgery have shown their efficacy. During intensive care, the risk of pulmonary edema is insignificant when less than 4 liters are given daily. Apart from cases of severe renal failure, they increase diuresis and reduce post-operative renal failure. Their use in appropriate cases permits economies in blood transfusion.", "contents": "[Use of electrolyte solutions in surgery (author's transl)]. The use of isotonic saline and Ringer's lactate in surgery has become widespread following the work of Shires on the sequestration of extra-cellular fluid in hemorrhagic shock. Their use both in military and civilian practice and during hemodilutions in cardiac surgery have shown their efficacy. During intensive care, the risk of pulmonary edema is insignificant when less than 4 liters are given daily. Apart from cases of severe renal failure, they increase diuresis and reduce post-operative renal failure. Their use in appropriate cases permits economies in blood transfusion."}
{"id": "PMID:468928", "title": "[Surgery of acute aortic dissection by use of a biological glue. Experimental study (author's transl)].", "content": "The improvement in surgical technic in the treatment of dissecting aneurysm of the aorta should permit a reduction in mortality and secondary complications. With this object, an attempt to stick together the two parts of the aneurysm with biological glue, was submitted to an experimental study. The operative protocol included on the one hand, creation of a dissecting aneurysm in the thorax of the dog, and secondly, repair of the latter with biological glue of variable composition. 27 dogs were thus operated on : -- 4 dissections were treated by simple suture (control group); -- in 23 cases, the two parts of the dissecting aneurysm were stuck together with gelatin-resorcin glue which was polymerised either by formaldehyde alone, or by a mixture of glutaraldehyde-glycerinaldehyde or by a mixture formaldehyde-glutaraldehyde. The best results of adhesiveness and tissue tolerance were obtained with the latter mixture.", "contents": "[Surgery of acute aortic dissection by use of a biological glue. Experimental study (author's transl)]. The improvement in surgical technic in the treatment of dissecting aneurysm of the aorta should permit a reduction in mortality and secondary complications. With this object, an attempt to stick together the two parts of the aneurysm with biological glue, was submitted to an experimental study. The operative protocol included on the one hand, creation of a dissecting aneurysm in the thorax of the dog, and secondly, repair of the latter with biological glue of variable composition. 27 dogs were thus operated on : -- 4 dissections were treated by simple suture (control group); -- in 23 cases, the two parts of the dissecting aneurysm were stuck together with gelatin-resorcin glue which was polymerised either by formaldehyde alone, or by a mixture of glutaraldehyde-glycerinaldehyde or by a mixture formaldehyde-glutaraldehyde. The best results of adhesiveness and tissue tolerance were obtained with the latter mixture."}
{"id": "PMID:468929", "title": "[G2 technic : a simplified procedure of reconstitution of the angle of His, applied to the treatment of hiatus hernia and malposition of the greater tuberosity (242 cases) (author's transl)].", "content": "A simple procedure of reconstitution of the angle of His applied to the treatment of hiatus hernia and malposition of the greater tuberosity is reported. Applied to 242 cases, it presents compared with the classical procedure several advantages : easy to carry out, including no complex manoevre, it does not endanger the vagus nerve and maintains firmly the anastomosis. Radiological control and, above all, the clinical results were excellent or good in 96% of cases, suggesting that this new procedure is perfectly valid.", "contents": "[G2 technic : a simplified procedure of reconstitution of the angle of His, applied to the treatment of hiatus hernia and malposition of the greater tuberosity (242 cases) (author's transl)]. A simple procedure of reconstitution of the angle of His applied to the treatment of hiatus hernia and malposition of the greater tuberosity is reported. Applied to 242 cases, it presents compared with the classical procedure several advantages : easy to carry out, including no complex manoevre, it does not endanger the vagus nerve and maintains firmly the anastomosis. Radiological control and, above all, the clinical results were excellent or good in 96% of cases, suggesting that this new procedure is perfectly valid."}
{"id": "PMID:468930", "title": "[Surgery of urethral stenosis, 162 cases (author's transl)].", "content": "The authors report a series of 194 operations for urethral stenosis, carried out in 162 patients between 1971 and 1977, and give the indications for various operative procedures. In the absence of chronic prostatitis and changes in the corpora spongiosa, limited stenosis should now be treated by endoscopic urethrotomy. Extensive stenosis or penoscrotal stenosis should be treated by vaginal urethroplasty, the other cases by urethroplasty in two stages. The quality of the results which may and ought to be satisfactory in 8 cases out of 10 depends on the judicious choice of the operative procedure, care in execution and the quality of the postoperative supervision.", "contents": "[Surgery of urethral stenosis, 162 cases (author's transl)]. The authors report a series of 194 operations for urethral stenosis, carried out in 162 patients between 1971 and 1977, and give the indications for various operative procedures. In the absence of chronic prostatitis and changes in the corpora spongiosa, limited stenosis should now be treated by endoscopic urethrotomy. Extensive stenosis or penoscrotal stenosis should be treated by vaginal urethroplasty, the other cases by urethroplasty in two stages. The quality of the results which may and ought to be satisfactory in 8 cases out of 10 depends on the judicious choice of the operative procedure, care in execution and the quality of the postoperative supervision."}
{"id": "PMID:468931", "title": "[Acute pancreatitis and post-traumatic pseudocysts of the pancreas. Report of 12 cases (author's transl)].", "content": "The authors report 12 personal cases and a bibliographic study showing the authenticity of acute pancreatitis and pancreatic pseudo-cyst following trauma. The clinico-pathological aspects, the course and the treatment are not specific. The medico-legal aspect is the most important for the secondary or late manifestations raise the problem of the imputability to the accident. Surgeons and judicial experts should be aware of the post-traumatic risk.", "contents": "[Acute pancreatitis and post-traumatic pseudocysts of the pancreas. Report of 12 cases (author's transl)]. The authors report 12 personal cases and a bibliographic study showing the authenticity of acute pancreatitis and pancreatic pseudo-cyst following trauma. The clinico-pathological aspects, the course and the treatment are not specific. The medico-legal aspect is the most important for the secondary or late manifestations raise the problem of the imputability to the accident. Surgeons and judicial experts should be aware of the post-traumatic risk."}
{"id": "PMID:468955", "title": "A novel procedure for the determination of tricyclic antidepressants in plasma by use of high performance liquid chromatography.", "content": "A novel method for the determination of the antidepressant 3-(1-chloro-5-H-dibenzo [a,d] cycloheptene-5-ylidene)-N,N-dimethylpropylamine-N-oxide hydrochloride and its metabolites by use of high performance liquid chromatography was developed. The procedure is applicable to the assay of other similar drugs in biological samples. The method involves extraction of the unchanged drug and its metabolites from plasma, back-extraction into diluted phosphoric acid and re-extraction into an organic phase. Separation is performed on a silica gel column with an acidic mobile phase, containing sodium dodecyl sulfate as ion-pairing agent. The quantitation is carried out by UV detection. The procedure allows the determination of plasma levels down to about 5 ng/ml of the unchanged drug and its metabolites, respectively, when 1 ml of plasma is used. The plasma levels of two volunteers were determined after a single oral dose of the drug.", "contents": "A novel procedure for the determination of tricyclic antidepressants in plasma by use of high performance liquid chromatography. A novel method for the determination of the antidepressant 3-(1-chloro-5-H-dibenzo [a,d] cycloheptene-5-ylidene)-N,N-dimethylpropylamine-N-oxide hydrochloride and its metabolites by use of high performance liquid chromatography was developed. The procedure is applicable to the assay of other similar drugs in biological samples. The method involves extraction of the unchanged drug and its metabolites from plasma, back-extraction into diluted phosphoric acid and re-extraction into an organic phase. Separation is performed on a silica gel column with an acidic mobile phase, containing sodium dodecyl sulfate as ion-pairing agent. The quantitation is carried out by UV detection. The procedure allows the determination of plasma levels down to about 5 ng/ml of the unchanged drug and its metabolites, respectively, when 1 ml of plasma is used. The plasma levels of two volunteers were determined after a single oral dose of the drug."}
{"id": "PMID:468972", "title": "Short term growth in boys with delayed puberty after diagnostic human chorionic gonadotropin administration.", "content": "Using a sensitive measuring device, 3-day hCG administration (Pregnyl; 1500 IU daily) was shown to temporarily increase ulnar growth velocity from prepubertal (0.40 +/- 0.35 mm/3 weeks to pubertal values (1.1 +/- 0.64 mm/3 weeks) in 10 boys with delayed puberty. This growth-promoting effect of diagnostic hCG administration, which was demonstrable for 3--9 weeks, was associated with an overt rise in plasma testosterone from 129 +/- 126 to 818 +/- 419 ng/100 ml and an approximate doubling of the serum alkaline phosphatase activities from 193 +/- 46 to 376 +/- 115 U/liter, suggesting an initiated growth spurt.", "contents": "Short term growth in boys with delayed puberty after diagnostic human chorionic gonadotropin administration. Using a sensitive measuring device, 3-day hCG administration (Pregnyl; 1500 IU daily) was shown to temporarily increase ulnar growth velocity from prepubertal (0.40 +/- 0.35 mm/3 weeks to pubertal values (1.1 +/- 0.64 mm/3 weeks) in 10 boys with delayed puberty. This growth-promoting effect of diagnostic hCG administration, which was demonstrable for 3--9 weeks, was associated with an overt rise in plasma testosterone from 129 +/- 126 to 818 +/- 419 ng/100 ml and an approximate doubling of the serum alkaline phosphatase activities from 193 +/- 46 to 376 +/- 115 U/liter, suggesting an initiated growth spurt."}
{"id": "PMID:468973", "title": "Male pseudohermaphroditism secondary to 17 beta-hydroxysteroid dehydrogenase deficiency: gender role change with puberty.", "content": "A 31-yr-old male pseudohermaphrodite is reported with 17 beta-hydroxysteroid dehydrogenase deficiency. Laboratory data revealed a plasma testosterone of 228 ng/100 ml, a plasma androstenedione of 620 ng/100 ml, and an abnormal androstenedione to testosterone ratio. Plasma estradiol was 4.6 ng/100 ml and plasma estrone was 22 ng/100 ml. This subject was born in a hospital, incontrovertibly declared to be a female, and unambiguously raised as a girl by his parents for the first 17 yr of his life. At age 14 yr, he was able to change to a male gender role with ease. As an adult, he is a well adjusted, happily married man with a successful professional career. Surgical correction of bilateral cryptorchidism and hypospadias was carried out at age 14 yr. At age 30 yr, he developed a teratocarcinoma-seminoma of the right testis with retroperitoneal node metastases. After orchiectomy and retroperitoneal node dissection, he was placed on chemotherapy and is presently free of metastases.", "contents": "Male pseudohermaphroditism secondary to 17 beta-hydroxysteroid dehydrogenase deficiency: gender role change with puberty. A 31-yr-old male pseudohermaphrodite is reported with 17 beta-hydroxysteroid dehydrogenase deficiency. Laboratory data revealed a plasma testosterone of 228 ng/100 ml, a plasma androstenedione of 620 ng/100 ml, and an abnormal androstenedione to testosterone ratio. Plasma estradiol was 4.6 ng/100 ml and plasma estrone was 22 ng/100 ml. This subject was born in a hospital, incontrovertibly declared to be a female, and unambiguously raised as a girl by his parents for the first 17 yr of his life. At age 14 yr, he was able to change to a male gender role with ease. As an adult, he is a well adjusted, happily married man with a successful professional career. Surgical correction of bilateral cryptorchidism and hypospadias was carried out at age 14 yr. At age 30 yr, he developed a teratocarcinoma-seminoma of the right testis with retroperitoneal node metastases. After orchiectomy and retroperitoneal node dissection, he was placed on chemotherapy and is presently free of metastases."}
{"id": "PMID:468974", "title": "Evidence that testosterone can suppress pituitary gonadotropin secretion independently of peripheral aromatization.", "content": "Testosterone (T) was given to normal men with and without the concomitant administration of the aromatase inhibitor, delta 1-testolactone (Teslac), to examine the role of peripheral aromatization of T in gonadotropin regulation. When T was administered alone by continuous iv infusion (15 mg/day for 4 days), serum T increased 3-fold (P less than 0.01) and estradiol (E) increased by 50% (P less than 0.01). These changes were associated with a 50% decrease in serum LH and FSH concentrations (P less than 0.01). When T was infused into men taking Teslac (2000 mg/day), serum T levels doubled (P less than 0.01), but E levels did not change (13.4 +/- 1.5 vs. 13.5 +/- 1.0 pg/ml; P = NS). This pattern of plasma steroids, increased T and unchanged E, was also associated with significantly decreased serum LH and FSH concentrations (14.5 +/- 0.4 vs. 8.0 + 0.4 mIU/ml and 9.9 +/- 2.5 vs. 5.8 +/- 0.1 mIU/ml, respectively; P less than 0.01). These data support the hypothesis that T or one of its metabolites can modulate LH and FSH secretion independently of peripheral aromatization to E.", "contents": "Evidence that testosterone can suppress pituitary gonadotropin secretion independently of peripheral aromatization. Testosterone (T) was given to normal men with and without the concomitant administration of the aromatase inhibitor, delta 1-testolactone (Teslac), to examine the role of peripheral aromatization of T in gonadotropin regulation. When T was administered alone by continuous iv infusion (15 mg/day for 4 days), serum T increased 3-fold (P less than 0.01) and estradiol (E) increased by 50% (P less than 0.01). These changes were associated with a 50% decrease in serum LH and FSH concentrations (P less than 0.01). When T was infused into men taking Teslac (2000 mg/day), serum T levels doubled (P less than 0.01), but E levels did not change (13.4 +/- 1.5 vs. 13.5 +/- 1.0 pg/ml; P = NS). This pattern of plasma steroids, increased T and unchanged E, was also associated with significantly decreased serum LH and FSH concentrations (14.5 +/- 0.4 vs. 8.0 + 0.4 mIU/ml and 9.9 +/- 2.5 vs. 5.8 +/- 0.1 mIU/ml, respectively; P less than 0.01). These data support the hypothesis that T or one of its metabolites can modulate LH and FSH secretion independently of peripheral aromatization to E."}
{"id": "PMID:468975", "title": "Alterations in serum cortisol and its binding characteristics in anorexia nervosa.", "content": "The binding capacity and apparent binding affinity of corticosteroid-binding globulin (CBG) for cortisol was studied in 20 women hospitalized for anorexia nervosa. The binding capacity for cortisol, as determined kinetically at 0 C by the method of Scatchard, was similar in anorectic patients and control subjects, with mean values of 26.9 and 21.9 microgram/100 ml, respectively. The mean apparent affinity constant of CBG for cortisol was found to be significantly (P less than 0.01) lower (14.1 X 10(8) M-1) than that in control subjects (22.3 X 10(8) M-1) and was similar in blood drawn at 0830 and 2000 h. Mean morning total serum cortisol concentrations were higher in anorectic women (23.3 microgram/100 ml) than in controls (10.0 microgram/100 ml; P less than 0.001) and frequently exceeded the binding capacity of CBG. Diurnal variation was present. Mean serum 17 beta-estradiol levels were low (40.0 pg/ml) and serum progesterone was essentially absent, but mean serum testosterone levels were within the normal range. Eight patients who were retested after weight gain, when they were between 76--100% of ideal body weight, exhibited no significant changes in serum steroids or in the binding capacity or apparent affinity of CBG for cortisol.", "contents": "Alterations in serum cortisol and its binding characteristics in anorexia nervosa. The binding capacity and apparent binding affinity of corticosteroid-binding globulin (CBG) for cortisol was studied in 20 women hospitalized for anorexia nervosa. The binding capacity for cortisol, as determined kinetically at 0 C by the method of Scatchard, was similar in anorectic patients and control subjects, with mean values of 26.9 and 21.9 microgram/100 ml, respectively. The mean apparent affinity constant of CBG for cortisol was found to be significantly (P less than 0.01) lower (14.1 X 10(8) M-1) than that in control subjects (22.3 X 10(8) M-1) and was similar in blood drawn at 0830 and 2000 h. Mean morning total serum cortisol concentrations were higher in anorectic women (23.3 microgram/100 ml) than in controls (10.0 microgram/100 ml; P less than 0.001) and frequently exceeded the binding capacity of CBG. Diurnal variation was present. Mean serum 17 beta-estradiol levels were low (40.0 pg/ml) and serum progesterone was essentially absent, but mean serum testosterone levels were within the normal range. Eight patients who were retested after weight gain, when they were between 76--100% of ideal body weight, exhibited no significant changes in serum steroids or in the binding capacity or apparent affinity of CBG for cortisol."}
{"id": "PMID:468976", "title": "Oxytocin in maternal circulation and amniotic fluid during pregnancy.", "content": "Oxytocin (OT) was measured by a specific and sensitive RIA in plasma and amniotic fluid throughout pregnancy. OT was detectable in 84.5% of 362 maternal plasma samples and showed a slow and fluctuating increase towards term with a significant sharp peak at 39 weeks of gestation. There was a highly significant correlation between mean plasma OT and the week of gestation (r = 0.5419, P less than 0.005). The minute to minute variability in plasma OT during pregnancy and labor in 7 subjects showed episodic release of OT with two or three spurts per 10 min, with the amplitude of the spurts being greater during labor. Serial maternal plasma OT throughout pregnancy in 10 patients revealed good concentrations of OT (greater than 10 pg/ml) in patients who subsequently had spontaneous labor and no intrapartum uterine dysfunction. Poor (less than 10 pg/ml) or undetectable OT levels were found in patients who subsequently developed intrapartum uterine dysfunction which necessitated cesarean section. OT was detectable in 89.7% or 87 amniotic fluid samples, with a mean +/- SE of 7.8 +/- 3.6 pg/ml at 14--15 weeks, 43.9 +/- 14.7 pg/ml at 40 weeks, and 30.8 +/- 10.5 pg/ml at 41--42 weeks. The significance of these findings is discussed in relation to the role and origin of OT in pregnancy and parturition.", "contents": "Oxytocin in maternal circulation and amniotic fluid during pregnancy. Oxytocin (OT) was measured by a specific and sensitive RIA in plasma and amniotic fluid throughout pregnancy. OT was detectable in 84.5% of 362 maternal plasma samples and showed a slow and fluctuating increase towards term with a significant sharp peak at 39 weeks of gestation. There was a highly significant correlation between mean plasma OT and the week of gestation (r = 0.5419, P less than 0.005). The minute to minute variability in plasma OT during pregnancy and labor in 7 subjects showed episodic release of OT with two or three spurts per 10 min, with the amplitude of the spurts being greater during labor. Serial maternal plasma OT throughout pregnancy in 10 patients revealed good concentrations of OT (greater than 10 pg/ml) in patients who subsequently had spontaneous labor and no intrapartum uterine dysfunction. Poor (less than 10 pg/ml) or undetectable OT levels were found in patients who subsequently developed intrapartum uterine dysfunction which necessitated cesarean section. OT was detectable in 89.7% or 87 amniotic fluid samples, with a mean +/- SE of 7.8 +/- 3.6 pg/ml at 14--15 weeks, 43.9 +/- 14.7 pg/ml at 40 weeks, and 30.8 +/- 10.5 pg/ml at 41--42 weeks. The significance of these findings is discussed in relation to the role and origin of OT in pregnancy and parturition."}
{"id": "PMID:468977", "title": "Calcitonin in nonthyroidal cancer.", "content": "A blind prospective study was undertaken to determine the use of calcitonin (CT) as a tumor marker. After final diagnosis, results revealed elevated plasma CT (greater than 150 pg/ml) in common cancers as follows: lung, 38%; colon, 24%; breast, 38%; pancreas, 42%; and gastric, 30%. Fifty-eight percent of oat cell carcinomas were associated with elevated plasma CT. CT immunoreactivity was detected in 14% of tumor extracts and was not detectable in normal tissue other than thyroid. Hypercalcemia was not the cause of hypercalcitonemia. Incubation studies of [125I]human CT in cancer plasma and tumor extracts demonstrated that measurements were not an artifact of label degradation. In a survey of control patients with nonneoplastic disease, elevated CT was noted in renal failure, acute gastrointestinal bleeding, and in some patients with chronic obstructive lung disease. In conclusion, plasma CT is elevated in a substantial proportion of common neoplasms and is useful as a tumor marker.", "contents": "Calcitonin in nonthyroidal cancer. A blind prospective study was undertaken to determine the use of calcitonin (CT) as a tumor marker. After final diagnosis, results revealed elevated plasma CT (greater than 150 pg/ml) in common cancers as follows: lung, 38%; colon, 24%; breast, 38%; pancreas, 42%; and gastric, 30%. Fifty-eight percent of oat cell carcinomas were associated with elevated plasma CT. CT immunoreactivity was detected in 14% of tumor extracts and was not detectable in normal tissue other than thyroid. Hypercalcemia was not the cause of hypercalcitonemia. Incubation studies of [125I]human CT in cancer plasma and tumor extracts demonstrated that measurements were not an artifact of label degradation. In a survey of control patients with nonneoplastic disease, elevated CT was noted in renal failure, acute gastrointestinal bleeding, and in some patients with chronic obstructive lung disease. In conclusion, plasma CT is elevated in a substantial proportion of common neoplasms and is useful as a tumor marker."}
{"id": "PMID:468980", "title": "Calcium homeostasis in diabetes mellitus.", "content": "Experimentally diabetic rats have low serum 1,25-dihydroxyvitamin D, intestinal malabsorption of calcium, secondary hyperparathyroidism, and bone loss. To examine the hypothesis that abnormalities similar to those in the diabetic rat might explain human diabetic osteopenia, we studied calcium metabolism in 40 healthy control and 82 diabetic patients aged 18--75 yr [47 untreated: fasting plasma glucose (mean +/- SE), 267 +/- 8 mg/dl; 19 treated but hyperglycemic: glucose 305 +/- 24 mg/dl; 16 treated and in better control: glucose, 146 +/- 8 mg/dl]. Serum total calcium, ionic calcium, immunoreactive parathyroid hormone (Arnaud method, GP-1M and CH-12M antisera), 25-hydroxyvitamin D (Haddad method), and 1,25-dihydroxyvitamin D (Lambert method) concentrations were normal in all 3 groups of diabetics and were not significantly different from values in the control group. We determined absorption of calcium from the intestine by a double isotope method (100 mg Ca carrier; normal range, 40--80%) in 11 control and 13 untreated, uncontrolled diabetics (mean plasma glucose, 285 +/- 17 mg/dl). Absorption of calcium in controls was 60 +/- 3% and in diabetics was 56 +/- 3% (not significantly different). We have found no derangement of calcium metabolism in adults with insulin-requiring juvenile- and adult-onset diabetes regardless of treatment status. The experimental diabetic rat model does not appear to be useful for determining the pathogenesis of adult human diabetic osteopenia.", "contents": "Calcium homeostasis in diabetes mellitus. Experimentally diabetic rats have low serum 1,25-dihydroxyvitamin D, intestinal malabsorption of calcium, secondary hyperparathyroidism, and bone loss. To examine the hypothesis that abnormalities similar to those in the diabetic rat might explain human diabetic osteopenia, we studied calcium metabolism in 40 healthy control and 82 diabetic patients aged 18--75 yr [47 untreated: fasting plasma glucose (mean +/- SE), 267 +/- 8 mg/dl; 19 treated but hyperglycemic: glucose 305 +/- 24 mg/dl; 16 treated and in better control: glucose, 146 +/- 8 mg/dl]. Serum total calcium, ionic calcium, immunoreactive parathyroid hormone (Arnaud method, GP-1M and CH-12M antisera), 25-hydroxyvitamin D (Haddad method), and 1,25-dihydroxyvitamin D (Lambert method) concentrations were normal in all 3 groups of diabetics and were not significantly different from values in the control group. We determined absorption of calcium from the intestine by a double isotope method (100 mg Ca carrier; normal range, 40--80%) in 11 control and 13 untreated, uncontrolled diabetics (mean plasma glucose, 285 +/- 17 mg/dl). Absorption of calcium in controls was 60 +/- 3% and in diabetics was 56 +/- 3% (not significantly different). We have found no derangement of calcium metabolism in adults with insulin-requiring juvenile- and adult-onset diabetes regardless of treatment status. The experimental diabetic rat model does not appear to be useful for determining the pathogenesis of adult human diabetic osteopenia."}
{"id": "PMID:468981", "title": "Effect of flutamide on estradiol metabolism.", "content": "The effect of flutamide, a potent nonsteroidal antiandrogen, on the metabolism of iv tracers of [3H]estradiol was studied in five patients with advanced prostate cancer. The drug produced no change in the percentage of the injected radioactivity recovered in urine or in the glucuronide or nonglucuronide conjugate fractions. Of the five individual metabolites that were quantitated, estrone, estradiol, and estriol were unaffected by flutamide, but the drug caused striking decreases in conversion of estradiol to 2-hydroxyestrone (4.0% vs. 7.4%) (P less than 0.005) and 2-methoxyestrone (1.1% vs. 2.6%; P less than 0.05); every one of the patients showed a marked fall in recovery of both of these compounds. This depression of the formation of 2-oxygenated metabolites is reminiscent of the findings in liver disease; the same abnormality occurs regularly in cirrhosis and frequently in extrahepatic biliary obstruction. Taken together with our previous studies of the effects of flutamide on testosterone and cortisol metabolism, this study demonstrates that flutamide produces multiple functional, reversible, cirrhosis-like disturbances of steroid metabolism. Because these disturbances are universal in the patients studied regardless of whether they had clinical responses to flutamide, we doubt that the steroid metabolic changes play a role in the therapeutic effect of the drug.", "contents": "Effect of flutamide on estradiol metabolism. The effect of flutamide, a potent nonsteroidal antiandrogen, on the metabolism of iv tracers of [3H]estradiol was studied in five patients with advanced prostate cancer. The drug produced no change in the percentage of the injected radioactivity recovered in urine or in the glucuronide or nonglucuronide conjugate fractions. Of the five individual metabolites that were quantitated, estrone, estradiol, and estriol were unaffected by flutamide, but the drug caused striking decreases in conversion of estradiol to 2-hydroxyestrone (4.0% vs. 7.4%) (P less than 0.005) and 2-methoxyestrone (1.1% vs. 2.6%; P less than 0.05); every one of the patients showed a marked fall in recovery of both of these compounds. This depression of the formation of 2-oxygenated metabolites is reminiscent of the findings in liver disease; the same abnormality occurs regularly in cirrhosis and frequently in extrahepatic biliary obstruction. Taken together with our previous studies of the effects of flutamide on testosterone and cortisol metabolism, this study demonstrates that flutamide produces multiple functional, reversible, cirrhosis-like disturbances of steroid metabolism. Because these disturbances are universal in the patients studied regardless of whether they had clinical responses to flutamide, we doubt that the steroid metabolic changes play a role in the therapeutic effect of the drug."}
{"id": "PMID:468982", "title": "Corpus luteum--conceptus--follicle relationships during the fertile cycle in rhesus monkeys: pregnancy maintenance despite early luteal removal.", "content": "To determine how early in pregnancy cyclic follicle growth is interrupted, 6 pregnant monkeys were luteectomized (CLX) on day 29 or 30 of the cycle (approximately 1 week after implantation) to abbreviate the fertile cycle to approximately the length of nonfertile cycles. Unexpectedly, 3 of 6 monkeys remained pregnant despite early removal of luteal support. In the remainder, which aborted, the next ovulation was delayed beyond the interval typically observed after CLX in nonfertile cycles. Two of 4 other monkeys ovariectomized on day 29 also maintained their pregnancies. Our findings demonstrate that: 1) in 5 of 10 monkeys, the conceptus could survive without luteal support within a week after luteal rescue, and 2) secretion(s) of the conceptus also contribute to an arrest of cyclic follicle growth early in pregnancy.", "contents": "Corpus luteum--conceptus--follicle relationships during the fertile cycle in rhesus monkeys: pregnancy maintenance despite early luteal removal. To determine how early in pregnancy cyclic follicle growth is interrupted, 6 pregnant monkeys were luteectomized (CLX) on day 29 or 30 of the cycle (approximately 1 week after implantation) to abbreviate the fertile cycle to approximately the length of nonfertile cycles. Unexpectedly, 3 of 6 monkeys remained pregnant despite early removal of luteal support. In the remainder, which aborted, the next ovulation was delayed beyond the interval typically observed after CLX in nonfertile cycles. Two of 4 other monkeys ovariectomized on day 29 also maintained their pregnancies. Our findings demonstrate that: 1) in 5 of 10 monkeys, the conceptus could survive without luteal support within a week after luteal rescue, and 2) secretion(s) of the conceptus also contribute to an arrest of cyclic follicle growth early in pregnancy."}
{"id": "PMID:468983", "title": "Specific radioimmunoassay of human beta-endorphin in unextracted plasma.", "content": "With an antiserum against human beta-endorphin (beta-EP) crossreacting less than 2% with human beta-lipotropin (beta-LPH) by weight we have developed a radioimmunoassay that can detect 1 pg beta-EP in diluted raw plasma. In a.m. fasting plasma of 14 normal subjects beta-EP ranged from less than 5 to 45 pg/ml. beta-EP was elevated in untreated, but normal in successfully treated Cushing's disease; undetectable in a patient with adrenal adenoma; extremely high in Nelson's syndrome; and elevated in a patient with bronchogenic carcinoma before, but undetectable after tumor resection. In subjects with intact hypothalamic-pituitary-adrenal axis, beta-EP was undetectable after dexamethasone and increased after metyrapone administration and insulin-induced hypoglycemia. beta-EP concentration was considerably lower in serum than in simultaneously collected plasma, but increased in serum left unfrozen for several hours after clot removal. Thus, beta-EP behaves like a hormone responding to the same stimuli as ACTH and beta-LPH and blood appears to contain enzymes both generating and destroying immunoreactive beta-EP.", "contents": "Specific radioimmunoassay of human beta-endorphin in unextracted plasma. With an antiserum against human beta-endorphin (beta-EP) crossreacting less than 2% with human beta-lipotropin (beta-LPH) by weight we have developed a radioimmunoassay that can detect 1 pg beta-EP in diluted raw plasma. In a.m. fasting plasma of 14 normal subjects beta-EP ranged from less than 5 to 45 pg/ml. beta-EP was elevated in untreated, but normal in successfully treated Cushing's disease; undetectable in a patient with adrenal adenoma; extremely high in Nelson's syndrome; and elevated in a patient with bronchogenic carcinoma before, but undetectable after tumor resection. In subjects with intact hypothalamic-pituitary-adrenal axis, beta-EP was undetectable after dexamethasone and increased after metyrapone administration and insulin-induced hypoglycemia. beta-EP concentration was considerably lower in serum than in simultaneously collected plasma, but increased in serum left unfrozen for several hours after clot removal. Thus, beta-EP behaves like a hormone responding to the same stimuli as ACTH and beta-LPH and blood appears to contain enzymes both generating and destroying immunoreactive beta-EP."}
{"id": "PMID:468984", "title": "Acromegaly and vitamin D metabolism: effect of bromocriptine treatment.", "content": "Fifteen acromegalic subjects were found to have elevated plasma levels of both 1,25(OH)2-vitamin D, 65 +/- 23 (SD) pg/ml [normal 33 + 15 pg/ml (SD)] and 24,25(OH)2-vitamin D, 6.8 +/- 1.6 (SD) ng/ml [normal 3.4 + 1.2 ng/ml (SD)]. Treatment with bromocriptine for 6 months reduced the plasma 1,25(OH)2-vitamin D3 level to 40 +/- 13 (SD) pg/ml, p less than 0.01 and the 24,25(OH)2-vitamin D level to 5.4 +/- 1.7 (SD) ng/ml, p less than 0.05.", "contents": "Acromegaly and vitamin D metabolism: effect of bromocriptine treatment. Fifteen acromegalic subjects were found to have elevated plasma levels of both 1,25(OH)2-vitamin D, 65 +/- 23 (SD) pg/ml [normal 33 + 15 pg/ml (SD)] and 24,25(OH)2-vitamin D, 6.8 +/- 1.6 (SD) ng/ml [normal 3.4 + 1.2 ng/ml (SD)]. Treatment with bromocriptine for 6 months reduced the plasma 1,25(OH)2-vitamin D3 level to 40 +/- 13 (SD) pg/ml, p less than 0.01 and the 24,25(OH)2-vitamin D level to 5.4 +/- 1.7 (SD) ng/ml, p less than 0.05."}
{"id": "PMID:468985", "title": "Plasma acetate turnover and oxidation.", "content": "Plasma acetate turnover and oxidation were determined in 11 healthy subjects by the constant infusion of a trace amount of [1-14C]acetate for 6 h. The subjects ages ranged from 22 to 57 yr. There was a positive correlation (P less than 0.001) between plasma acetate concentration and turnover rate, and a negative correlation (P less than 0.001) between turnover and age. The plasma acetate concentration in the subjects 22--28 yr old was 0.17 vs. 0.13 mM (P less than 0.02) in subjects 40--57 yr old. The plasma acetate turnover rate was also greater in the younger age group (8.23 +/- 0.66 vs. 4.98 +/- 0.64 mumol/min . kg, P less than 0.01). Approximately 90% of the plasma acetate turnover was immediately oxidized to CO2 in both age groups, however, 13.2 +/- 0.89% of the CO2 output in the younger group was derived from plasma acetate oxidation compared to 7.9 +/- 0.94% in the older group (P less than 0.01). The mean plasma acetate concentration, turnover, and oxidation in six cancer patients 47--63 yr old were similar to the values observed in the age-matched healthy subjects. Uptake or output of acetate by various tissues was measured by arterial-venous plasma acetate concentration differences. In seven of eight subjects undergoing elective surgery, the arterial-portal venous concentration difference was negative, which indicated that the gastrointestinal tract can contribute to plasma acetate production. Uptake of plasma acetate by both the leg and liver appeared to be dictated by the arterial acetate concentration. Net production of acetate by both the leg and liver was most often observed at arterial plasma acetate concentrations less than 0.08 mM.", "contents": "Plasma acetate turnover and oxidation. Plasma acetate turnover and oxidation were determined in 11 healthy subjects by the constant infusion of a trace amount of [1-14C]acetate for 6 h. The subjects ages ranged from 22 to 57 yr. There was a positive correlation (P less than 0.001) between plasma acetate concentration and turnover rate, and a negative correlation (P less than 0.001) between turnover and age. The plasma acetate concentration in the subjects 22--28 yr old was 0.17 vs. 0.13 mM (P less than 0.02) in subjects 40--57 yr old. The plasma acetate turnover rate was also greater in the younger age group (8.23 +/- 0.66 vs. 4.98 +/- 0.64 mumol/min . kg, P less than 0.01). Approximately 90% of the plasma acetate turnover was immediately oxidized to CO2 in both age groups, however, 13.2 +/- 0.89% of the CO2 output in the younger group was derived from plasma acetate oxidation compared to 7.9 +/- 0.94% in the older group (P less than 0.01). The mean plasma acetate concentration, turnover, and oxidation in six cancer patients 47--63 yr old were similar to the values observed in the age-matched healthy subjects. Uptake or output of acetate by various tissues was measured by arterial-venous plasma acetate concentration differences. In seven of eight subjects undergoing elective surgery, the arterial-portal venous concentration difference was negative, which indicated that the gastrointestinal tract can contribute to plasma acetate production. Uptake of plasma acetate by both the leg and liver appeared to be dictated by the arterial acetate concentration. Net production of acetate by both the leg and liver was most often observed at arterial plasma acetate concentrations less than 0.08 mM."}
{"id": "PMID:468986", "title": "Properdin factor D: effects on thrombin-induced platelet aggregation.", "content": "Factor D, when preincubated with platelet suspensions, at concentrations as low as 1.2 micrograms/ml, inhibited thrombin-induced platelet aggregation. No inhibition of collagen or arachidonic acid-induced platelet aggregation was found. Inhibition occurred, but to a lesser extent, when thrombin and factor D were added to platelets at the same time. No inhibition occurred when factor D was added after thrombin. Thrombin was able to overcome inhibition by factor D by increasing its concentration. Diisopropyl-phosphorofluoridate-inactivated factor D also inhibited thrombin-induced platelet aggregation so that enzymatic activity of factor D was not required for inhibition. Factor D absorbed with hirudin coupled to Sepharose 6B showed no decrease in inhibitory capacity. 125I-Factor D bound to platelets in a manner suggesting an equilibrium reaction similar to thrombin. At low factor D input, binding was linear, whereas at higher input, binding began to approach saturation. Binding of 125I-labeled thrombin to platelets was inhibited by factor D. Analysis of these data show that factor D does not alter the total number of thrombin molecules which bind to the platelet surface at saturation. However, the dissociation constant for thrombin is altered from 2.78 to 6.90 nM in the presence of factor D (20 micrograms/ml). Factor D is thus a competitive inhibitor of thrombin binding, although the affinity of factor D for the platelet thrombin receptor is much less than that of thrombin. These phenomena occur at physiologic concentrations of factor D. Therefore, factor D may function in vivo as an inhibitor of platelet aggregation.", "contents": "Properdin factor D: effects on thrombin-induced platelet aggregation. Factor D, when preincubated with platelet suspensions, at concentrations as low as 1.2 micrograms/ml, inhibited thrombin-induced platelet aggregation. No inhibition of collagen or arachidonic acid-induced platelet aggregation was found. Inhibition occurred, but to a lesser extent, when thrombin and factor D were added to platelets at the same time. No inhibition occurred when factor D was added after thrombin. Thrombin was able to overcome inhibition by factor D by increasing its concentration. Diisopropyl-phosphorofluoridate-inactivated factor D also inhibited thrombin-induced platelet aggregation so that enzymatic activity of factor D was not required for inhibition. Factor D absorbed with hirudin coupled to Sepharose 6B showed no decrease in inhibitory capacity. 125I-Factor D bound to platelets in a manner suggesting an equilibrium reaction similar to thrombin. At low factor D input, binding was linear, whereas at higher input, binding began to approach saturation. Binding of 125I-labeled thrombin to platelets was inhibited by factor D. Analysis of these data show that factor D does not alter the total number of thrombin molecules which bind to the platelet surface at saturation. However, the dissociation constant for thrombin is altered from 2.78 to 6.90 nM in the presence of factor D (20 micrograms/ml). Factor D is thus a competitive inhibitor of thrombin binding, although the affinity of factor D for the platelet thrombin receptor is much less than that of thrombin. These phenomena occur at physiologic concentrations of factor D. Therefore, factor D may function in vivo as an inhibitor of platelet aggregation."}
{"id": "PMID:468987", "title": "Intestinal calcium absorption and serum vitamin D metabolites in normal subjects and osteoporotic patients: effect of age and dietary calcium.", "content": "Intestinal calcium absorption assessed by a double-isotope method, decreased significantly with aging in 94 normal subjects (r = -0.22, P < 0.025). In 52 untreated patients with postmenopausal osteoporosis, calcium absorption was significantly lower than normal when either age or habitual calcium intake was used as a covariable (P < 0.001). Serum 25-hydroxyvitamin D (25-OH-D) and 1,25-dihydroxyvitamin D (1,25(OH)(2)D) were measured in 44 normal subjects and 27 osteoporotic patients. For all normals, calcium absorption and serum 1,25(OH)(2)D were positively correlated (r = 0.50, P < 0.001). In nonelderly normal subjects (ages 30-65 yr), dietary calcium intake correlated inversely with both calcium absorption (r = -0.39, P < 0.01) and with serum 1,25(OH)(2)D (r = -0.50, P < 0.01). Both osteoporotic patients and elderly normal subjects (ages 65-90 yr) differed from nonelderly normals in that these correlations were not present. In addition although serum 25-OH-D was normal, serum 1,25(OH)(2)D was significantly decreased in both osteoporotic patients and elderly normals (P < 0.001). In osteoporotic patients, calcium absorption increased significantly (P < 0.001) after 7 d administration of a small dose (0.4 mug/d) of synthetic 1,25(OH)(2)D(3). In osteoporotics mean serum immunoreactive parathyroid hormone was either normal (COOH-terminal assay) or low (NH(2)-terminal assay) relative to age-matched controls, and mean serum phosphate was increased. The data suggest that inadequate metabolism of 25-OH-D to 1,25(OH)(2)D contributes significantly to decreased calcium absorption and adaptation in both osteoporotics and elderly normal subjects. In patients with osteoporosis this abnormality could result from a decrease in factors that normally stimulate 1,25(OH)(2)D production, such as the decreased parathyroid hormone secretion and increased serum phosphate demonstrated in this group. In elderly subjects a primary abnormality in metabolism of 25-OH-D to 1,25(OH)(2)D, analagous to that seen in aging rats, cannot be excluded.", "contents": "Intestinal calcium absorption and serum vitamin D metabolites in normal subjects and osteoporotic patients: effect of age and dietary calcium. Intestinal calcium absorption assessed by a double-isotope method, decreased significantly with aging in 94 normal subjects (r = -0.22, P < 0.025). In 52 untreated patients with postmenopausal osteoporosis, calcium absorption was significantly lower than normal when either age or habitual calcium intake was used as a covariable (P < 0.001). Serum 25-hydroxyvitamin D (25-OH-D) and 1,25-dihydroxyvitamin D (1,25(OH)(2)D) were measured in 44 normal subjects and 27 osteoporotic patients. For all normals, calcium absorption and serum 1,25(OH)(2)D were positively correlated (r = 0.50, P < 0.001). In nonelderly normal subjects (ages 30-65 yr), dietary calcium intake correlated inversely with both calcium absorption (r = -0.39, P < 0.01) and with serum 1,25(OH)(2)D (r = -0.50, P < 0.01). Both osteoporotic patients and elderly normal subjects (ages 65-90 yr) differed from nonelderly normals in that these correlations were not present. In addition although serum 25-OH-D was normal, serum 1,25(OH)(2)D was significantly decreased in both osteoporotic patients and elderly normals (P < 0.001). In osteoporotic patients, calcium absorption increased significantly (P < 0.001) after 7 d administration of a small dose (0.4 mug/d) of synthetic 1,25(OH)(2)D(3). In osteoporotics mean serum immunoreactive parathyroid hormone was either normal (COOH-terminal assay) or low (NH(2)-terminal assay) relative to age-matched controls, and mean serum phosphate was increased. The data suggest that inadequate metabolism of 25-OH-D to 1,25(OH)(2)D contributes significantly to decreased calcium absorption and adaptation in both osteoporotics and elderly normal subjects. In patients with osteoporosis this abnormality could result from a decrease in factors that normally stimulate 1,25(OH)(2)D production, such as the decreased parathyroid hormone secretion and increased serum phosphate demonstrated in this group. In elderly subjects a primary abnormality in metabolism of 25-OH-D to 1,25(OH)(2)D, analagous to that seen in aging rats, cannot be excluded."}
{"id": "PMID:468988", "title": "Measurement, characterization, and source of somatostatin-like immunoreactivity in human amniotic fluid.", "content": "Somatostatin-like immunoreactivity (SLI) is widely distributed in tissues and biological fluids. To determine whether SLI is also present in amniotic fluid, samples obtained by amniocentesis from 30 normal and 27 abnormal pregnancies were studied by radioimmunoassay. Direct incubation of [(125)I-Tyr(1)]tetradecapeptide somatostatin (SRIF) with amniotic fluid resulted in 89% tracer degradation. Damage was reduced to <5% when samples were acidified and boiled before the assay. With this technique, SLI was detectable in all normal amniotic fluid samples; the mean level at 15-20 wk of gestation (320+/-55 pg/ml, n = 15) being 4.5 times higher than the mean at 32-43 wk (70+/-12 pg/ml, n = 15) (P < 0.001). In cases of preeclampsia (n = 6), gestational diabetes (n = 5), anencephaly (n = 1), and meningomyelocele (n = 1), SLI values were in the normal range, but in one juvenile diabetic and one patient with chronic renal failure, SLI was undetectable (<10 pg/ml). In a pair of monochorionic diamniotic twins, SLI levels were very different (33 and 197 pg/ml), which suggests that fetal factors are more important than materno-placental ones in determining amniotic fluid SLI. Serial dilutions of amniotic fluid showed parallelism with standard SRIF. When concentrates of pooled amniotic fluid were chromatographed on Sephadex G-25 columns, all SLI eluted in the void volume ahead of SRIF even after treatment with 8 M urea and dithiothreitol. This \"big\" SLI incubated in amniotic fluid showed 100% stability over 24 h at 37 degrees C, whereas SRIF was rapidly inactivated (t((1/2)) congruent with 7 min). Extracts of placenta and fetal membranes contained no SLI, but small amounts (6-20% of total amniotic fluid SLI) were found in cells from fresh fluid. Radioimmunoassay of SLI in extracts of seven paired cord arterial and venous plasma samples showed no arteriovenous gradient consistent with fetal origin of cord blood SLI. It is concluded that (a) amniotic fluid contains SLI which is of fetal origin and (b) normal levels vary with gestational age. The SLI has a higher molecular weight (>/=5,000) and is more stable in amniotic fluid than SRIF.", "contents": "Measurement, characterization, and source of somatostatin-like immunoreactivity in human amniotic fluid. Somatostatin-like immunoreactivity (SLI) is widely distributed in tissues and biological fluids. To determine whether SLI is also present in amniotic fluid, samples obtained by amniocentesis from 30 normal and 27 abnormal pregnancies were studied by radioimmunoassay. Direct incubation of [(125)I-Tyr(1)]tetradecapeptide somatostatin (SRIF) with amniotic fluid resulted in 89% tracer degradation. Damage was reduced to <5% when samples were acidified and boiled before the assay. With this technique, SLI was detectable in all normal amniotic fluid samples; the mean level at 15-20 wk of gestation (320+/-55 pg/ml, n = 15) being 4.5 times higher than the mean at 32-43 wk (70+/-12 pg/ml, n = 15) (P < 0.001). In cases of preeclampsia (n = 6), gestational diabetes (n = 5), anencephaly (n = 1), and meningomyelocele (n = 1), SLI values were in the normal range, but in one juvenile diabetic and one patient with chronic renal failure, SLI was undetectable (<10 pg/ml). In a pair of monochorionic diamniotic twins, SLI levels were very different (33 and 197 pg/ml), which suggests that fetal factors are more important than materno-placental ones in determining amniotic fluid SLI. Serial dilutions of amniotic fluid showed parallelism with standard SRIF. When concentrates of pooled amniotic fluid were chromatographed on Sephadex G-25 columns, all SLI eluted in the void volume ahead of SRIF even after treatment with 8 M urea and dithiothreitol. This \"big\" SLI incubated in amniotic fluid showed 100% stability over 24 h at 37 degrees C, whereas SRIF was rapidly inactivated (t((1/2)) congruent with 7 min). Extracts of placenta and fetal membranes contained no SLI, but small amounts (6-20% of total amniotic fluid SLI) were found in cells from fresh fluid. Radioimmunoassay of SLI in extracts of seven paired cord arterial and venous plasma samples showed no arteriovenous gradient consistent with fetal origin of cord blood SLI. It is concluded that (a) amniotic fluid contains SLI which is of fetal origin and (b) normal levels vary with gestational age. The SLI has a higher molecular weight (>/=5,000) and is more stable in amniotic fluid than SRIF."}
{"id": "PMID:468989", "title": "Heterogeneity of DNA fragments associated with the sickle-globin gene.", "content": "We have examined the genetic polymorphism previously reported to be associated with the sickle-cell (beta s) gene. The polymorphism involves an alteration of the DNA sequence 3' to the beta-globin gene as detected with the restriction endonuclease, Hpa I. In normal individuals, the beta-globin gene is contained within a DNA fragment of 7.6 kilobases (kb), whereas 87% of individuals with sickle-cell anemia have been reported to have the beta s-gene associated with a 13.0-kb Hpa I fragment. We have studied this polymorphism in 31 New York Black individuals homozygous for sickle-cell anemia to ascertain its genetic and biochemical significance and to evaluate its potential use in the prenatal diagnosis of sickle-cell disease. Our results show only a 58% association of the beta s-gene and the 13.0-kb Hpa I fragment, as well as the presence of additional variants involving the Hpa I site. In addition, the 13.0-kb fragment is also found associated with the beta c- and beta A-genes. Thus, the Hpa I polymorphism probably represents a change in DNA not specifically associated with the beta s-gene, and appears to antedate the beta s-and beta c-mutations.", "contents": "Heterogeneity of DNA fragments associated with the sickle-globin gene. We have examined the genetic polymorphism previously reported to be associated with the sickle-cell (beta s) gene. The polymorphism involves an alteration of the DNA sequence 3' to the beta-globin gene as detected with the restriction endonuclease, Hpa I. In normal individuals, the beta-globin gene is contained within a DNA fragment of 7.6 kilobases (kb), whereas 87% of individuals with sickle-cell anemia have been reported to have the beta s-gene associated with a 13.0-kb Hpa I fragment. We have studied this polymorphism in 31 New York Black individuals homozygous for sickle-cell anemia to ascertain its genetic and biochemical significance and to evaluate its potential use in the prenatal diagnosis of sickle-cell disease. Our results show only a 58% association of the beta s-gene and the 13.0-kb Hpa I fragment, as well as the presence of additional variants involving the Hpa I site. In addition, the 13.0-kb fragment is also found associated with the beta c- and beta A-genes. Thus, the Hpa I polymorphism probably represents a change in DNA not specifically associated with the beta s-gene, and appears to antedate the beta s-and beta c-mutations."}
{"id": "PMID:468990", "title": "Elevated cholesterol and bile acid synthesis in a young patient with homozygous familial hypercholesterolemia.", "content": "Cholesterol balance studies were carried out twice in a young male patient with homozygous familial hypercholesterolemia. At 13 mo, cholesterol balance in this patient averaged 31.3 mg/kg per d, and bile acid excretion was 12.0 mg/kg per d; at 3 yr, results were similar, 27.3 and 15.5 mg/kg per d for cholesterol balance and bile acids, respectively. A normal boy of 3 yr was also studied for comparison with the second study in our patient. Cholesterol balance and bile acid outputs in the normal child were 11.5 and 3.3 mg/kg per d, respectively. Thus, in comparison with the normal child, the patient with homozygous familial hypercholesterolemia had a marked increase in synthesis of cholesterol and bile acids. Although synthesis of bile acids was high in this patient, the fraction of newly synthesized cholesterol converted into bile acids (40-56%) was in the normal range; this suggests that the enhanced output of bile acids was secondary to an increased synthesis of cholesterol and not to malabsorption of bile acids, which likely would have produced a higher fractional conversion. Although our patient has been studied at a younger age than any reported in the literature, two similar children 5 and 6 yr of age were also observed to have elevated cholesterol synthesis. This finding contrasts with those in older children with the homozygous as well as heterozygous forms of this disease who appear to have normal synthesis of cholesterol and bile acids. Therefore, increased synthesis of cholesterol seems to be characteristic of early homozygous familial hypercholesterolemia, and may be a manifestation of a loss of feedback inhibition of cholesterol synthesis secondary to an absence of specific cell-surface receptors for low density lipoproteins. However, as children with this disease grow older, other mechanisms may come into play to restore cholesterol synthesis to normal levels.", "contents": "Elevated cholesterol and bile acid synthesis in a young patient with homozygous familial hypercholesterolemia. Cholesterol balance studies were carried out twice in a young male patient with homozygous familial hypercholesterolemia. At 13 mo, cholesterol balance in this patient averaged 31.3 mg/kg per d, and bile acid excretion was 12.0 mg/kg per d; at 3 yr, results were similar, 27.3 and 15.5 mg/kg per d for cholesterol balance and bile acids, respectively. A normal boy of 3 yr was also studied for comparison with the second study in our patient. Cholesterol balance and bile acid outputs in the normal child were 11.5 and 3.3 mg/kg per d, respectively. Thus, in comparison with the normal child, the patient with homozygous familial hypercholesterolemia had a marked increase in synthesis of cholesterol and bile acids. Although synthesis of bile acids was high in this patient, the fraction of newly synthesized cholesterol converted into bile acids (40-56%) was in the normal range; this suggests that the enhanced output of bile acids was secondary to an increased synthesis of cholesterol and not to malabsorption of bile acids, which likely would have produced a higher fractional conversion. Although our patient has been studied at a younger age than any reported in the literature, two similar children 5 and 6 yr of age were also observed to have elevated cholesterol synthesis. This finding contrasts with those in older children with the homozygous as well as heterozygous forms of this disease who appear to have normal synthesis of cholesterol and bile acids. Therefore, increased synthesis of cholesterol seems to be characteristic of early homozygous familial hypercholesterolemia, and may be a manifestation of a loss of feedback inhibition of cholesterol synthesis secondary to an absence of specific cell-surface receptors for low density lipoproteins. However, as children with this disease grow older, other mechanisms may come into play to restore cholesterol synthesis to normal levels."}
{"id": "PMID:468991", "title": "Human plasma protein C: isolation, characterization, and mechanism of activation by alpha-thrombin.", "content": "Protein C is a vitamin K-dependent protein, which exists in bovine plasma as a precursor of a serine protease. In this study, protein C was isolated to homogeneity from human plasma by barium citrate adsorption and elution, ammonium sulfate fractionation, DEAE-Sephadex chromatography, dextran sulfate agarose chromatography, and preparative polyacrylamide gel electrophoresis. Human protein C (M(r) = 62,000) contains 23% carbohydrate and is composed of a light chain (M(r) = 21,000) and a heavy chain (M(r) = 41,000) held together by a disulfide bond(s). The light chain has an amino-terminal sequence of Ala-Asn-Ser-Phe-Leu- and the heavy chain has an aminoterminal sequence of Asp-Pro-Glu-Asp-Gln. The residues that are identical to bovine protein C are underlined. Incubation of human protein C with human alpha-thrombin at an enzyme to substrate weight ratio of 1:50 resulted in the formation of activated protein C, an enzyme with serine amidase activity. In the activation reaction, the apparent molecular weight of the heavy chain decreased from 41,000 to 40,000 as determined by gel electrophoresis in the presence of sodium dodecyl sulfate. No apparent change in the molecular weight of the light chain was observed in the activation process. The heavy chain of human activated protein C also contains the active-site serine residue as evidenced by its ability to react with radiolabeled diisopropyl fluorophosphate. Human activated protein C markedly prolongs the kaolin-cephalin clotting time of human plasma, but not that of bovine plasma. The amidolytic and anticoagulant activities of human activated protein C were completely obviated by prior incubation of the enzyme with diisopropyl fluorophosphate. These results indicate that human protein C, like its bovine counterpart, exists in plasma as a zymogen and is converted to a serine protease by limited proteolysis with attendant anticoagulant activity.", "contents": "Human plasma protein C: isolation, characterization, and mechanism of activation by alpha-thrombin. Protein C is a vitamin K-dependent protein, which exists in bovine plasma as a precursor of a serine protease. In this study, protein C was isolated to homogeneity from human plasma by barium citrate adsorption and elution, ammonium sulfate fractionation, DEAE-Sephadex chromatography, dextran sulfate agarose chromatography, and preparative polyacrylamide gel electrophoresis. Human protein C (M(r) = 62,000) contains 23% carbohydrate and is composed of a light chain (M(r) = 21,000) and a heavy chain (M(r) = 41,000) held together by a disulfide bond(s). The light chain has an amino-terminal sequence of Ala-Asn-Ser-Phe-Leu- and the heavy chain has an aminoterminal sequence of Asp-Pro-Glu-Asp-Gln. The residues that are identical to bovine protein C are underlined. Incubation of human protein C with human alpha-thrombin at an enzyme to substrate weight ratio of 1:50 resulted in the formation of activated protein C, an enzyme with serine amidase activity. In the activation reaction, the apparent molecular weight of the heavy chain decreased from 41,000 to 40,000 as determined by gel electrophoresis in the presence of sodium dodecyl sulfate. No apparent change in the molecular weight of the light chain was observed in the activation process. The heavy chain of human activated protein C also contains the active-site serine residue as evidenced by its ability to react with radiolabeled diisopropyl fluorophosphate. Human activated protein C markedly prolongs the kaolin-cephalin clotting time of human plasma, but not that of bovine plasma. The amidolytic and anticoagulant activities of human activated protein C were completely obviated by prior incubation of the enzyme with diisopropyl fluorophosphate. These results indicate that human protein C, like its bovine counterpart, exists in plasma as a zymogen and is converted to a serine protease by limited proteolysis with attendant anticoagulant activity."}
{"id": "PMID:468992", "title": "Relationship between alveolar PO2 and the rate of p-nitroanisole O-demethylation by the cytochrome P-450 pathway in isolated rabbit lungs.", "content": "The relationship between alveolar PO2 and the rate of O-demethylation of p-nitroanisole, a model substrate for cytochrome P-450 -linked mixed-function oxidation, was evaluated in the isolated rabbit lung perfused with Krebs-Ringer bicarbonate buffer. The appearance of the product, p-nitrophenol, in the pulmonary perfusate was measured spectrophotometrically, The PO2 of the ventilating gas was varied with an accurate gas mixing pump and measured with an electrochemical O2 analyzer. In control lungs ventilated with 5% CO2 in air, the rate of p-nitrophenol production was approximately equal to 3.1 +/- 0.04 (mean +/- SE; n = 9) mumol/h per g dry wt. p-Nitrophenol production was unaltered when O2 in the ventilating gas was decreased to 1%, but it was depressed reversibly when alveolar O2 WAS 0.1% OR LESS AND WAS ABOLISHED DURING VENTILATION WITH 0.005% O2. The rate of the reaction was inhibited by 50% when alveolar PO2 was 0.3 mm Hg representing and intracellular [O2] OF approximately equal to muM. In the presence of metyrapone (0.1--1 mM), an inhibitor of cytochrome P-450-dependent reactions, p-nitrophenol production was 0.07--0.17 mumol/h per g dry wt. Ventilation of lungs with varying CO concentration in 20% O2 resulted in 50% inhibition of p-nitrophenol production when CO concentration was 10% (CO/O2 = 0.5). These results indicated that O-demethylation of p-nitroanisole by the lung is a cytochrome P-450-dependent reaction and that its rate is not affected until alveolar PO2 is less than 1 mm Hg.", "contents": "Relationship between alveolar PO2 and the rate of p-nitroanisole O-demethylation by the cytochrome P-450 pathway in isolated rabbit lungs. The relationship between alveolar PO2 and the rate of O-demethylation of p-nitroanisole, a model substrate for cytochrome P-450 -linked mixed-function oxidation, was evaluated in the isolated rabbit lung perfused with Krebs-Ringer bicarbonate buffer. The appearance of the product, p-nitrophenol, in the pulmonary perfusate was measured spectrophotometrically, The PO2 of the ventilating gas was varied with an accurate gas mixing pump and measured with an electrochemical O2 analyzer. In control lungs ventilated with 5% CO2 in air, the rate of p-nitrophenol production was approximately equal to 3.1 +/- 0.04 (mean +/- SE; n = 9) mumol/h per g dry wt. p-Nitrophenol production was unaltered when O2 in the ventilating gas was decreased to 1%, but it was depressed reversibly when alveolar O2 WAS 0.1% OR LESS AND WAS ABOLISHED DURING VENTILATION WITH 0.005% O2. The rate of the reaction was inhibited by 50% when alveolar PO2 was 0.3 mm Hg representing and intracellular [O2] OF approximately equal to muM. In the presence of metyrapone (0.1--1 mM), an inhibitor of cytochrome P-450-dependent reactions, p-nitrophenol production was 0.07--0.17 mumol/h per g dry wt. Ventilation of lungs with varying CO concentration in 20% O2 resulted in 50% inhibition of p-nitrophenol production when CO concentration was 10% (CO/O2 = 0.5). These results indicated that O-demethylation of p-nitroanisole by the lung is a cytochrome P-450-dependent reaction and that its rate is not affected until alveolar PO2 is less than 1 mm Hg."}
{"id": "PMID:468993", "title": "Mechanism of action of colchicine in acute urate crystal-induced arthritis.", "content": "Phagocytosis of urate crystals by human or rabbit neutrophils induces the synthesis and release of a glycoprotein, the crystal-induced chemotactic factor (CCF), which is chemotactically active both in vitro and in vivo. It has been proposed that CCF is a prime mediator of the acute gouty attack. Colchicine has been shown to decrease the production and release of this factor in vitro. In these studies, colchicine, at nonleukopenic doses, is shown to abrogate the acute arthritis induced by monosodium urate crystals in rabbits, but to have no effect upon the arthritis induced by the injection of the purified cell-derived chemotactic factor. Serum colchicine levels were 0.48-0.58 muM at 30 min and 0.12-0.3 muM at 90 min after intravenous injection of 0.2 mg/kg colchicine. Peripheral blood polymorphonuclear leukocytes obtained from colchicine-treated animals migrated normally towards a chemotactic stimulus but failed to produce CCF after phagocytosis of monosodium urate crystals. The dialyzed synovial fluid from rabbits injected with microcrystalline sodium urate contained chemotactic activity that was not present when animals were also given intravenous colchicine or injected intra-articularly with the chemotactic factor formyl-methionyl-leucyl-phenylalanine. Furthermore, the synovial fluid from rabbits injected with microcrystalline sodium urate significantly decreased (125)I-CCF binding to neutrophils. The binding of (125)I-CCF to its neutrophil receptor was not significantly reduced by the synovial fluid of colchicine-treated rabbits nor by the synovial fluid of control rabbits injected with the chemotactic factor formyl-methionyl-leucyl-phenylalanine. Colchicine (10 and 0.1 muM) was shown to have no effect upon the binding of (125)I-CCF to its cell receptor.", "contents": "Mechanism of action of colchicine in acute urate crystal-induced arthritis. Phagocytosis of urate crystals by human or rabbit neutrophils induces the synthesis and release of a glycoprotein, the crystal-induced chemotactic factor (CCF), which is chemotactically active both in vitro and in vivo. It has been proposed that CCF is a prime mediator of the acute gouty attack. Colchicine has been shown to decrease the production and release of this factor in vitro. In these studies, colchicine, at nonleukopenic doses, is shown to abrogate the acute arthritis induced by monosodium urate crystals in rabbits, but to have no effect upon the arthritis induced by the injection of the purified cell-derived chemotactic factor. Serum colchicine levels were 0.48-0.58 muM at 30 min and 0.12-0.3 muM at 90 min after intravenous injection of 0.2 mg/kg colchicine. Peripheral blood polymorphonuclear leukocytes obtained from colchicine-treated animals migrated normally towards a chemotactic stimulus but failed to produce CCF after phagocytosis of monosodium urate crystals. The dialyzed synovial fluid from rabbits injected with microcrystalline sodium urate contained chemotactic activity that was not present when animals were also given intravenous colchicine or injected intra-articularly with the chemotactic factor formyl-methionyl-leucyl-phenylalanine. Furthermore, the synovial fluid from rabbits injected with microcrystalline sodium urate significantly decreased (125)I-CCF binding to neutrophils. The binding of (125)I-CCF to its neutrophil receptor was not significantly reduced by the synovial fluid of colchicine-treated rabbits nor by the synovial fluid of control rabbits injected with the chemotactic factor formyl-methionyl-leucyl-phenylalanine. Colchicine (10 and 0.1 muM) was shown to have no effect upon the binding of (125)I-CCF to its cell receptor."}
{"id": "PMID:468994", "title": "Radioimmunochemical quantitation of human adenosine deaminase.", "content": "Markedly reduced or absent adenosine deaminase activity in man is associated with an autosomal recesive form of severe conbined immunodeficiency disease. To further define the genetic nature of this enzyme defect, we have quantitated immunologically active adenosine deaminase (CRM) in the hemolysate of homozygous deficient patients and their heterozygous parents. A highly specific radioimmunoassay was developed capable of detecting 0.05% of normal erythrocyte adenosine deaminase. Hemolysates from nine heterozygotes (five families) showed a wide range in CRM (32--100% of normal) and variable absolute specific activities with several being at least 1 SD BELOW THE NORMAL MEAN. Hemolysates from four unrelated patients showed less than 0.09% adenosine deaminase activity with CRM ranging from less than 0.06 to 5.6% of the normal mean. In conclusion, heterozygote and homozygote hemolysates from five of the eight families analyzed revealed variable levels of CRM suggesting heterogeneous genetic alteration or expression of the silent or defective allele(s) of adenosine deaminase.", "contents": "Radioimmunochemical quantitation of human adenosine deaminase. Markedly reduced or absent adenosine deaminase activity in man is associated with an autosomal recesive form of severe conbined immunodeficiency disease. To further define the genetic nature of this enzyme defect, we have quantitated immunologically active adenosine deaminase (CRM) in the hemolysate of homozygous deficient patients and their heterozygous parents. A highly specific radioimmunoassay was developed capable of detecting 0.05% of normal erythrocyte adenosine deaminase. Hemolysates from nine heterozygotes (five families) showed a wide range in CRM (32--100% of normal) and variable absolute specific activities with several being at least 1 SD BELOW THE NORMAL MEAN. Hemolysates from four unrelated patients showed less than 0.09% adenosine deaminase activity with CRM ranging from less than 0.06 to 5.6% of the normal mean. In conclusion, heterozygote and homozygote hemolysates from five of the eight families analyzed revealed variable levels of CRM suggesting heterogeneous genetic alteration or expression of the silent or defective allele(s) of adenosine deaminase."}
{"id": "PMID:468995", "title": "Excretion and metabolism of catecholamines by the isolated perfused rat kidney.", "content": "The excretion and metabolism of labeled epinephrine and norepinephrine by the isolated, perfused rat kidney were studied. The excretion of both catecholamines significantly exceeded the amount filtered, thus providing direct evidence of net tubular secretion. Renal clearance of epinephrine was significantly greater than that of norepinephrine. Tubular secretion was a linear function of the concentration of unbound catecholamine in the medium with no demonstrable tubular maximum at the concentrations studied. The isolated kidney removed catecholamines from the medium by metabolism as well as excretion in the urine. O-Methylation was the major metabolic route and O-methylated metabolites were rapidly excreted and concentrated in urine. Preferential excretion and metabolism of epinephrine were confirmed in double-label experiments in which [14C]epinephrine and [3H]norepinephrine were perfused together. The ratio of 14C:3H in urine exceeded that in perfusion medium for total radioactivity as well as for catecholamines and O-methylated amines. The present study thus provides direct evidence for (a) net tubular secretion of epinephrine and norepinephrine with a direct relationship between secretion and medium concentration; (b) significant renal metabolism of both epinephrine and norepinephrine with O-methylation as the major metabolic route; and (c) preferential excretion and metabolism of epinephrine.", "contents": "Excretion and metabolism of catecholamines by the isolated perfused rat kidney. The excretion and metabolism of labeled epinephrine and norepinephrine by the isolated, perfused rat kidney were studied. The excretion of both catecholamines significantly exceeded the amount filtered, thus providing direct evidence of net tubular secretion. Renal clearance of epinephrine was significantly greater than that of norepinephrine. Tubular secretion was a linear function of the concentration of unbound catecholamine in the medium with no demonstrable tubular maximum at the concentrations studied. The isolated kidney removed catecholamines from the medium by metabolism as well as excretion in the urine. O-Methylation was the major metabolic route and O-methylated metabolites were rapidly excreted and concentrated in urine. Preferential excretion and metabolism of epinephrine were confirmed in double-label experiments in which [14C]epinephrine and [3H]norepinephrine were perfused together. The ratio of 14C:3H in urine exceeded that in perfusion medium for total radioactivity as well as for catecholamines and O-methylated amines. The present study thus provides direct evidence for (a) net tubular secretion of epinephrine and norepinephrine with a direct relationship between secretion and medium concentration; (b) significant renal metabolism of both epinephrine and norepinephrine with O-methylation as the major metabolic route; and (c) preferential excretion and metabolism of epinephrine."}
{"id": "PMID:468996", "title": "Genetic control of the eighth component of complement.", "content": "Using isoelectric focusing in polyacrylamide gel and a hemolytic assay for development of patterns, extensive, structural polymorphism in human C8 has been delineated. Two alleles, C8A and C8B, have been identified in orientals, with gene frequencies of 0.655 and 0.345. In blacks, what appears to be a third common allele was found, so that frequencies were 0.692, 0.259, and 0.049 for C8A, C8B, and C8A1. In whites, C8A1 was rare with a frequency of 0.003, and frequencies for C8A and C8B were 0.649 and 0.349. Inheritance was autosomal codominant in family studies and the distribution of types in random unrelated populations fit the Hardy-Weinberg equilibrium in all groups. C8 allotypes have been determined for two previously studied families, each with a homozygous C8-deficient propositus. This study suggests that C8 deficiency is a silent or null allele of the C8 structural locus, and that half normal levels of C8 cannot be used as a single criterion for the establishment of heterozygous C8 deficiency. C8 allotypes, as well as 18 other autosomal markers, were also determined for 24 families. The C8 structural locus is not closely linked to these markers, including the human histocompatibility loci complex.", "contents": "Genetic control of the eighth component of complement. Using isoelectric focusing in polyacrylamide gel and a hemolytic assay for development of patterns, extensive, structural polymorphism in human C8 has been delineated. Two alleles, C8A and C8B, have been identified in orientals, with gene frequencies of 0.655 and 0.345. In blacks, what appears to be a third common allele was found, so that frequencies were 0.692, 0.259, and 0.049 for C8A, C8B, and C8A1. In whites, C8A1 was rare with a frequency of 0.003, and frequencies for C8A and C8B were 0.649 and 0.349. Inheritance was autosomal codominant in family studies and the distribution of types in random unrelated populations fit the Hardy-Weinberg equilibrium in all groups. C8 allotypes have been determined for two previously studied families, each with a homozygous C8-deficient propositus. This study suggests that C8 deficiency is a silent or null allele of the C8 structural locus, and that half normal levels of C8 cannot be used as a single criterion for the establishment of heterozygous C8 deficiency. C8 allotypes, as well as 18 other autosomal markers, were also determined for 24 families. The C8 structural locus is not closely linked to these markers, including the human histocompatibility loci complex."}
{"id": "PMID:468997", "title": "Deletion of the A gamma-globin gene in G gamma-delta beta-thalassemia.", "content": "In an individual homozygous for G gamma-delta beta-thalassemia, a physical alteration in gamma-globin gene organization was detected by restriction enzyme mapping. The data indicated that the absence of A gamma-globin chains resulted from extension of the DNA deletion from the delta beta-globin gene region into the gamma-globin gene region rather than a functional disturbance of gamma-gene expression.", "contents": "Deletion of the A gamma-globin gene in G gamma-delta beta-thalassemia. In an individual homozygous for G gamma-delta beta-thalassemia, a physical alteration in gamma-globin gene organization was detected by restriction enzyme mapping. The data indicated that the absence of A gamma-globin chains resulted from extension of the DNA deletion from the delta beta-globin gene region into the gamma-globin gene region rather than a functional disturbance of gamma-gene expression."}
{"id": "PMID:468999", "title": "Platelet function in beta-thalassaemia major.", "content": "Abnormal platelet aggregation was found in eight (44%) of 18 patients with beta-thalassaemia major and transfusional iron overload. The aggregation defect bore no correlation with the degree of hepatic fibrosis, liver function tests, whether or not splenectomy had been performed, the degree of iron overload, haematocrit, platelet count, serum vitamin E level, or leucocyte ascorbate concentration. Only three of the 18 patients showed prolonged bleeding times as well as abnormal platelet aggregation, and only one of these suffered clinically significant haemorrhage. The results show that a proportion of patients with beta-thalassaemia major have abnormal platelet function. It is possible, however, that the in vitro abnormality might be due partly to artefacts induced by manipulations required to remove the abnormal thalassaemic red cells, and this may explain the much lower incidence of significant haemorrhage.", "contents": "Platelet function in beta-thalassaemia major. Abnormal platelet aggregation was found in eight (44%) of 18 patients with beta-thalassaemia major and transfusional iron overload. The aggregation defect bore no correlation with the degree of hepatic fibrosis, liver function tests, whether or not splenectomy had been performed, the degree of iron overload, haematocrit, platelet count, serum vitamin E level, or leucocyte ascorbate concentration. Only three of the 18 patients showed prolonged bleeding times as well as abnormal platelet aggregation, and only one of these suffered clinically significant haemorrhage. The results show that a proportion of patients with beta-thalassaemia major have abnormal platelet function. It is possible, however, that the in vitro abnormality might be due partly to artefacts induced by manipulations required to remove the abnormal thalassaemic red cells, and this may explain the much lower incidence of significant haemorrhage."}
{"id": "PMID:469000", "title": "Platelet aggregation studies during transient hypoglycaemia: a potential method for evaluating platelet function.", "content": "The effect of acute hypoglycaemia on platelet function was examined in patients undergoing an insulin stress test. Enhanced platelet aggregation was observed in all cases but platelet count, platelet adenine nucleotides, and the plasma level of von Willebrand factor were unchanged overall. The onset of the hypoglycaemia-induced increase in platelet aggregation coincided with the lowest blood glucose levels recorded and with the clinical signs of adrenaline release. Increased platelet aggregation was maintained thereafter for the two-hour test period. There was no apparent correlation with changes in cortisol, growth hormone, and prolactin. No change in platelet function was observed after the administration of L-dopa. We suggest that the measurement of platelet aggregation during a standard insulin stress test may provide a means of evaluating platelet function in vivo and the influence of drugs thereon.", "contents": "Platelet aggregation studies during transient hypoglycaemia: a potential method for evaluating platelet function. The effect of acute hypoglycaemia on platelet function was examined in patients undergoing an insulin stress test. Enhanced platelet aggregation was observed in all cases but platelet count, platelet adenine nucleotides, and the plasma level of von Willebrand factor were unchanged overall. The onset of the hypoglycaemia-induced increase in platelet aggregation coincided with the lowest blood glucose levels recorded and with the clinical signs of adrenaline release. Increased platelet aggregation was maintained thereafter for the two-hour test period. There was no apparent correlation with changes in cortisol, growth hormone, and prolactin. No change in platelet function was observed after the administration of L-dopa. We suggest that the measurement of platelet aggregation during a standard insulin stress test may provide a means of evaluating platelet function in vivo and the influence of drugs thereon."}
{"id": "PMID:469001", "title": "Effect of dextran sulphates on thrombin activity.", "content": "To clarify the action of dextran sulphate, a heparin analogue, in the clotting of fibrinogen by thrombin, determinations were carried out on the clotting activity, the release of fibrinopeptides from fibrinogen, and the hydrolytic activity of thrombin against a peptide chromogenic substrate in the absence or presence of antithrombin III (heparin cofactor). It was shown that dextran sulphate itself inhibited thrombin activity, and its inhibition was dependent on the molecular weight and the sulphur content of the dextran sulphate. Although heparin markedly enhanced the antithrombin activity of antithrombin III, dextran sulphate did not activate antithrombin III.", "contents": "Effect of dextran sulphates on thrombin activity. To clarify the action of dextran sulphate, a heparin analogue, in the clotting of fibrinogen by thrombin, determinations were carried out on the clotting activity, the release of fibrinopeptides from fibrinogen, and the hydrolytic activity of thrombin against a peptide chromogenic substrate in the absence or presence of antithrombin III (heparin cofactor). It was shown that dextran sulphate itself inhibited thrombin activity, and its inhibition was dependent on the molecular weight and the sulphur content of the dextran sulphate. Although heparin markedly enhanced the antithrombin activity of antithrombin III, dextran sulphate did not activate antithrombin III."}
{"id": "PMID:469002", "title": "Increased interaction of vascular endothelium and leucocytes after administration of antiplatelet serum: role in the developing vascular defect.", "content": "Antiplatelet serum (APS) induced an increase in the stickiness of white cells which manifests itself in the increase in number of granulocytes rolling along or sticking to the venous endothelium. Lidocaine treatment prevented the increased stickiness of white cells and, at the same time, the microvascular haemorrhage developing after APS. It can be assumed that increased stickiness of white cells after APS may contribute to endothelial damage.", "contents": "Increased interaction of vascular endothelium and leucocytes after administration of antiplatelet serum: role in the developing vascular defect. Antiplatelet serum (APS) induced an increase in the stickiness of white cells which manifests itself in the increase in number of granulocytes rolling along or sticking to the venous endothelium. Lidocaine treatment prevented the increased stickiness of white cells and, at the same time, the microvascular haemorrhage developing after APS. It can be assumed that increased stickiness of white cells after APS may contribute to endothelial damage."}
{"id": "PMID:469003", "title": "Cytokinetic studies of crypts in convoluted human small-intestinal mucosa.", "content": "Forty-seven peroral biopsy specimens of duodenojejunal mucosa showing convolutions were obtained from patients with a variety of clinical disorders. These mucosal samples were divided into three groups according to the extent of the convolutions and the severity of the accompanying histopathological changes; the cytokinetic status of the crypts in the three groups was then analysed and compared. Those in which the mucosae were predominantly or totally convoluted (group 3) showed the most notable cytokinetic changes: crypts were hyperplastic and crypt cell production rate was markedly increased compared with the other two groups and with morphologically normal control mucosae. In the case of one patient with mucosal changes of group 3 severity, additional studies were carried out using vincristine to produce metaphase arrest. The cell cycle time of 27 hours was greatly shortened compared with a control value of 45 hours.We find that the presence of convolutions in small-intestinal mucosal biopsy specimens is accompanied by an increase in the rate of cell production from the crypts, which is presumably related directly or indirectly to the rate of loss of mature enterocytes from the surface of the mucosa. Furthermore, an increase in the proportion of convolutions may reflect an increase in the rates of cell production and cell loss. In the group 3 convoluted mucosae the cytokinetic profile of the crypts resembled that of some of the flat avillous coeliac mucosae previously studied although the rates of cell production did not reach the levels attained by the most productive of the flat coeliac mucosae.", "contents": "Cytokinetic studies of crypts in convoluted human small-intestinal mucosa. Forty-seven peroral biopsy specimens of duodenojejunal mucosa showing convolutions were obtained from patients with a variety of clinical disorders. These mucosal samples were divided into three groups according to the extent of the convolutions and the severity of the accompanying histopathological changes; the cytokinetic status of the crypts in the three groups was then analysed and compared. Those in which the mucosae were predominantly or totally convoluted (group 3) showed the most notable cytokinetic changes: crypts were hyperplastic and crypt cell production rate was markedly increased compared with the other two groups and with morphologically normal control mucosae. In the case of one patient with mucosal changes of group 3 severity, additional studies were carried out using vincristine to produce metaphase arrest. The cell cycle time of 27 hours was greatly shortened compared with a control value of 45 hours.We find that the presence of convolutions in small-intestinal mucosal biopsy specimens is accompanied by an increase in the rate of cell production from the crypts, which is presumably related directly or indirectly to the rate of loss of mature enterocytes from the surface of the mucosa. Furthermore, an increase in the proportion of convolutions may reflect an increase in the rates of cell production and cell loss. In the group 3 convoluted mucosae the cytokinetic profile of the crypts resembled that of some of the flat avillous coeliac mucosae previously studied although the rates of cell production did not reach the levels attained by the most productive of the flat coeliac mucosae."}
{"id": "PMID:469004", "title": "Adipose cell size in obese Africans: evidence against the existence of insulin resistance in some patients.", "content": "Aspects of adipose tissue cellularity were examined in 15 non-diabetic premenopausal African women with simple obesity living in Johannesburg. A smaller group of six non-obese Black women served as controls. Adipose tissue was obtained by biopsy from the deltoid, gluteal, and abdominal regions, and the mean fat cell size for each site was determined. Fasting plasma glucose, insulin, and lipid levels, and the glucose and insulin responses to a 100 g oral glucose load, in these subjects provided metabolic data for correlative analyses. As expected, the overall mean and regional adipocyte sizes were significantly larger in the overweight subjects. Significant regional variations in fat cell size were also seen, the gluteal region adipocytes being larger than those of other sites in both obese and non-obese women. A significant positive correlation was found between fat cell size and the percentage of ideal body weight. There was no significant relationship between adipocyte size, however, and any of the metabolic variables measured--notably basal or stimulated plasma insulin. Nearly half of the overweight women showed large adipocytes with normal plasma insulin concentrations. A proportion of African women with hypertrophic obesity do not appear to demonstrate any classical metabolic features of insulin resistance; this may be related partly to their high carbohydrate intake and unusual degree of physical activity. Our results do not, however, indicate that hyperinsulinaemia is completely absent in obese Black women.", "contents": "Adipose cell size in obese Africans: evidence against the existence of insulin resistance in some patients. Aspects of adipose tissue cellularity were examined in 15 non-diabetic premenopausal African women with simple obesity living in Johannesburg. A smaller group of six non-obese Black women served as controls. Adipose tissue was obtained by biopsy from the deltoid, gluteal, and abdominal regions, and the mean fat cell size for each site was determined. Fasting plasma glucose, insulin, and lipid levels, and the glucose and insulin responses to a 100 g oral glucose load, in these subjects provided metabolic data for correlative analyses. As expected, the overall mean and regional adipocyte sizes were significantly larger in the overweight subjects. Significant regional variations in fat cell size were also seen, the gluteal region adipocytes being larger than those of other sites in both obese and non-obese women. A significant positive correlation was found between fat cell size and the percentage of ideal body weight. There was no significant relationship between adipocyte size, however, and any of the metabolic variables measured--notably basal or stimulated plasma insulin. Nearly half of the overweight women showed large adipocytes with normal plasma insulin concentrations. A proportion of African women with hypertrophic obesity do not appear to demonstrate any classical metabolic features of insulin resistance; this may be related partly to their high carbohydrate intake and unusual degree of physical activity. Our results do not, however, indicate that hyperinsulinaemia is completely absent in obese Black women."}
{"id": "PMID:469005", "title": "An ultrastructural study of a nasal haemangioendothelioma.", "content": "A malignant haemangioendothelioma of the nose has been examined with the electron microscope. The tumour is predominantly poorly differentiated with only little evidence of its vascular ancestry identified. The cells are polygonal with a wide spectrum of organelles, including microfilaments. A conspicuous feature was the presence of cytoplasmic fenestrae developing into intracytoplasmic vacuolated spaces. The satisfactory progress of this patient is noted, and the value of electron microscopy in enabling an early and accurate diagnosis of this tumour to be made is emphasised.", "contents": "An ultrastructural study of a nasal haemangioendothelioma. A malignant haemangioendothelioma of the nose has been examined with the electron microscope. The tumour is predominantly poorly differentiated with only little evidence of its vascular ancestry identified. The cells are polygonal with a wide spectrum of organelles, including microfilaments. A conspicuous feature was the presence of cytoplasmic fenestrae developing into intracytoplasmic vacuolated spaces. The satisfactory progress of this patient is noted, and the value of electron microscopy in enabling an early and accurate diagnosis of this tumour to be made is emphasised."}
{"id": "PMID:469006", "title": "Adjusted calcium conflict resolved? Differing effects on plasma total calcium of changes in plasma albumin after venous stasis or myocardial infarction.", "content": "Others have challenged the concept of adjusting total plasma calcium for albumin concentration on the grounds that after the application of a tourniquet the increase in calcium for a given increase in albumin differs significantly between normal individuals. We have confirmed this finding. In contrast, we have found that after myocardial infarction the fall in calcium for a given fall in albumin does not differ significantly between patients. Thus the adjustment of calcium for albumin using a single equation remains valid in patients with changes in albumin due to disease. We recommend that for consistent results blood should be taken with the minimum of venous stasis even though the patient's calcium is to be adjusted for albumin.", "contents": "Adjusted calcium conflict resolved? Differing effects on plasma total calcium of changes in plasma albumin after venous stasis or myocardial infarction. Others have challenged the concept of adjusting total plasma calcium for albumin concentration on the grounds that after the application of a tourniquet the increase in calcium for a given increase in albumin differs significantly between normal individuals. We have confirmed this finding. In contrast, we have found that after myocardial infarction the fall in calcium for a given fall in albumin does not differ significantly between patients. Thus the adjustment of calcium for albumin using a single equation remains valid in patients with changes in albumin due to disease. We recommend that for consistent results blood should be taken with the minimum of venous stasis even though the patient's calcium is to be adjusted for albumin."}
{"id": "PMID:469007", "title": "The skin as the source of Acinetobacter and Moraxella species occurring in blood cultures.", "content": "A study was made of the flora of the skin in hospital inpatients and healthy people to demonstrate the presence of non-fermenting Gram-negative rods of the Acinetobacter and Moraxella group. These organisms were found to be present on the skin of 34.3% of inpatients and occurred even more commonly in those patients with kidney disease. It was also present on the skin of 20% of a group of healthy members of staff. This rather high rate of skin carriage is thought to account for the not infrequent occurrence of this organism in blood cultures.", "contents": "The skin as the source of Acinetobacter and Moraxella species occurring in blood cultures. A study was made of the flora of the skin in hospital inpatients and healthy people to demonstrate the presence of non-fermenting Gram-negative rods of the Acinetobacter and Moraxella group. These organisms were found to be present on the skin of 34.3% of inpatients and occurred even more commonly in those patients with kidney disease. It was also present on the skin of 20% of a group of healthy members of staff. This rather high rate of skin carriage is thought to account for the not infrequent occurrence of this organism in blood cultures."}
{"id": "PMID:469008", "title": "Class II (laminar flow) biological safety cabinet.", "content": "A microbiological survey of the effectiveness of class II (laminar flow) 'safety' cabinets found in the UK in the last six years is recorded. Only two of the nine units tested approached the containment of aerosols achieved by a good class I (exhaust protective) cabinet. The others were potentially hazardous if used with pathogenic material. The National Sanitation Foundation and the British Standards Institute have now laid down adequate specifications based on biological containment, and hopefully the conforming cabinets will be much better; even so, the purchase of a cabinet must be undertaken with care, and the cabinet requires frequent monitoring during use and after servicing.", "contents": "Class II (laminar flow) biological safety cabinet. A microbiological survey of the effectiveness of class II (laminar flow) 'safety' cabinets found in the UK in the last six years is recorded. Only two of the nine units tested approached the containment of aerosols achieved by a good class I (exhaust protective) cabinet. The others were potentially hazardous if used with pathogenic material. The National Sanitation Foundation and the British Standards Institute have now laid down adequate specifications based on biological containment, and hopefully the conforming cabinets will be much better; even so, the purchase of a cabinet must be undertaken with care, and the cabinet requires frequent monitoring during use and after servicing."}
{"id": "PMID:469009", "title": "HLA-linked C2 deficiency in a Dutch patient with systemic lupus erythematosus.", "content": "A patient with systemic lupus erythematosus was studied whose blood serum on repeated occasions showed undetectable levels of haemolytic omplement (C). A detailed investigation of individual C components in the serum of the proposita and her family revealed the absence of functional C2 in the patient and half-normal values in the relatives. C4 levels in the family, but not in the patient, were above normal, whereas the levels of factor B were low in all cases. No abnormalities were noted in C3, C9, or C1INH. Tissue typing showed linkage of the C2-deficiency gene with the HLA-A10/B18 and A9/B18 haplotypes. No linkage with red cell antigens and no relationship with plasma kallikrein levels was found.", "contents": "HLA-linked C2 deficiency in a Dutch patient with systemic lupus erythematosus. A patient with systemic lupus erythematosus was studied whose blood serum on repeated occasions showed undetectable levels of haemolytic omplement (C). A detailed investigation of individual C components in the serum of the proposita and her family revealed the absence of functional C2 in the patient and half-normal values in the relatives. C4 levels in the family, but not in the patient, were above normal, whereas the levels of factor B were low in all cases. No abnormalities were noted in C3, C9, or C1INH. Tissue typing showed linkage of the C2-deficiency gene with the HLA-A10/B18 and A9/B18 haplotypes. No linkage with red cell antigens and no relationship with plasma kallikrein levels was found."}
{"id": "PMID:469010", "title": "Reactogenicity and immunogenicity of a surface-antigen-adsorbed influenza virus vaccine in children.", "content": "An influenza virus vaccine containing the purified surface haemagglutinin and neuraminidase antigens of A/Victoria/75 and B/Hong Kong/73 viruses adsorbed to an aluminium hydroxide gel was assessed for reactogenicity and immunogenicity in children aged 4 to 11 years, since there is no influenza virus vaccine available for this age group. Significant serum haemagglutination-inhibiting antibody responses to the A/Victoria/75 and B/Hong Kong/73 haemagglutinin antigens present in the vaccine were observed in 47% and 35%, respectively, of the children vaccinated, with a single dose. The vaccine induced no significant local or systemic reactions.", "contents": "Reactogenicity and immunogenicity of a surface-antigen-adsorbed influenza virus vaccine in children. An influenza virus vaccine containing the purified surface haemagglutinin and neuraminidase antigens of A/Victoria/75 and B/Hong Kong/73 viruses adsorbed to an aluminium hydroxide gel was assessed for reactogenicity and immunogenicity in children aged 4 to 11 years, since there is no influenza virus vaccine available for this age group. Significant serum haemagglutination-inhibiting antibody responses to the A/Victoria/75 and B/Hong Kong/73 haemagglutinin antigens present in the vaccine were observed in 47% and 35%, respectively, of the children vaccinated, with a single dose. The vaccine induced no significant local or systemic reactions."}
{"id": "PMID:469011", "title": "Bordetella bronchicanis (bronchiseptica) infection in man: review and a case report.", "content": "Bordetella bronchicanis is a common respiratory tract commensal of mammals. Rarely it causes whooping cough in children. Compromised adults in hospitals may be colonised, and one terminal pneumonia is on record. The fatal pneumonia of a malnourished alcoholic described here was contracted at home.", "contents": "Bordetella bronchicanis (bronchiseptica) infection in man: review and a case report. Bordetella bronchicanis is a common respiratory tract commensal of mammals. Rarely it causes whooping cough in children. Compromised adults in hospitals may be colonised, and one terminal pneumonia is on record. The fatal pneumonia of a malnourished alcoholic described here was contracted at home."}
{"id": "PMID:469012", "title": "Radioimmunoassay of primary bile salts in serum.", "content": "Rapid, sensitive radioimmunoassays have been developed for the conjugated primary bile salts, cholate and chenodeoxycholate, using immunogens prepared by the mixed anhydride procedure. Antibodies produced showed equal specificity for glycine and taurine conjugates. Cross-reactivities were comparable with those from other published radioimmunoassays. The assays were routinely performed on unextracted sera and the concentrations correlated well with concentrations determined by gas-liquid chromatography. Accuracy, determined by the addition of bile salt to charcoal-extracted serum, and percision, determined by replicate analysis of a normal sample, were both less than +/- 10%. These figures are comparable with those obtained by both gas-liquid chromatography and other radioimmunoassays for bile salts. Normal sera were found to contain 0.49-1.32 mumol/l of cholate and 0.55-2.02 mumol/l of chenodeoxycholate. Serum concentrations in patients with liver disease were higher than this normal range. Three patients with mild liver distrubance were found to have one bile salt in the upper limit of normal, but in each case the other primary bile salt was outwith the normal range.", "contents": "Radioimmunoassay of primary bile salts in serum. Rapid, sensitive radioimmunoassays have been developed for the conjugated primary bile salts, cholate and chenodeoxycholate, using immunogens prepared by the mixed anhydride procedure. Antibodies produced showed equal specificity for glycine and taurine conjugates. Cross-reactivities were comparable with those from other published radioimmunoassays. The assays were routinely performed on unextracted sera and the concentrations correlated well with concentrations determined by gas-liquid chromatography. Accuracy, determined by the addition of bile salt to charcoal-extracted serum, and percision, determined by replicate analysis of a normal sample, were both less than +/- 10%. These figures are comparable with those obtained by both gas-liquid chromatography and other radioimmunoassays for bile salts. Normal sera were found to contain 0.49-1.32 mumol/l of cholate and 0.55-2.02 mumol/l of chenodeoxycholate. Serum concentrations in patients with liver disease were higher than this normal range. Three patients with mild liver distrubance were found to have one bile salt in the upper limit of normal, but in each case the other primary bile salt was outwith the normal range."}
{"id": "PMID:469013", "title": "Measurement of serum primary bile acid ratio by gas liquid chromatography and radioimmunoassay.", "content": "The application of a sensitive gas liquid chromatography (GLC) assay has enabled patients in different diagnostic groups to be differentiated on the basis of the ratio of the primary bile acids, cholic: chenodeoxycholic acid. Because this method is unsuitable for a routine clinical laboratory, the primary bile acid ratio (PBR) is determined by radioimmunoassay techniques (RIA), and the results were compared with GLC analysis. RIA provided comparable results in control subjects and patients with large-duct obstruction, but significantly higher PBR values were found in cirrhotic subjects.", "contents": "Measurement of serum primary bile acid ratio by gas liquid chromatography and radioimmunoassay. The application of a sensitive gas liquid chromatography (GLC) assay has enabled patients in different diagnostic groups to be differentiated on the basis of the ratio of the primary bile acids, cholic: chenodeoxycholic acid. Because this method is unsuitable for a routine clinical laboratory, the primary bile acid ratio (PBR) is determined by radioimmunoassay techniques (RIA), and the results were compared with GLC analysis. RIA provided comparable results in control subjects and patients with large-duct obstruction, but significantly higher PBR values were found in cirrhotic subjects."}
{"id": "PMID:469014", "title": "Performance of exhaust-protective (class I) biological 'safety' cabinets.", "content": "Nineteen open-fronted (class I) safety cabinets were subjected to spore containment and airflow tests, which suggested that a flow of 0.75 m/s was the minimum required for safe operation; further tests on three of the cabinets were repeated at different air speeds and confirmed this. The airflow is required to overcome the effect of laboratory or external air currents. Contamination of surfaces (including the operator's hands) by aerosols liberated inside the cabinets was investigated and found to depend more on turbulence within the cabinet (as from a restricted front opening) than on the number of air changes. The findings suggest that the product protection provided by a well-designed class I cabinet might equal that of a class II (laminar flow) unit.", "contents": "Performance of exhaust-protective (class I) biological 'safety' cabinets. Nineteen open-fronted (class I) safety cabinets were subjected to spore containment and airflow tests, which suggested that a flow of 0.75 m/s was the minimum required for safe operation; further tests on three of the cabinets were repeated at different air speeds and confirmed this. The airflow is required to overcome the effect of laboratory or external air currents. Contamination of surfaces (including the operator's hands) by aerosols liberated inside the cabinets was investigated and found to depend more on turbulence within the cabinet (as from a restricted front opening) than on the number of air changes. The findings suggest that the product protection provided by a well-designed class I cabinet might equal that of a class II (laminar flow) unit."}
{"id": "PMID:469015", "title": "Automated determination of red cell methaemoglobin reductase activity by a continuous-flow system for screening hereditary methaemoglobinaemia.", "content": "A flow diagram for the automated determination of ferricyanide reductase activity in red blood cells was prepared in the modules from AutoAnalyzer AA I (Technicon Instruments Inc). Ferricyanide reductase assay can be substituted for assay of cytochrome b5 reductase (EC 1.6.2.2), which plays a major role in reducing methaemoglobin in erythrocytes, and is defective specifically in the erythrocytes of patients with hereditary methaemoglobinaemia. The effective sampling rate of the analysis is 30/h, and less than 0.05 ml of whole blood is required. Interference of haemoglobin with absorption by potassium ferricyanide at 420 nm is effectively exculded by dialysis. This automated method was compared with the accepted diaphorase method, and it distinguished clearly the ferricyanide reductase activity of cord bloods from that of adult bloods. The activity of the blood from a patient with hereditary methaemoglobinaemia was only residual. It is suggested that the method is useful as a mass screening test for hereditary methaemoglobinaemia.", "contents": "Automated determination of red cell methaemoglobin reductase activity by a continuous-flow system for screening hereditary methaemoglobinaemia. A flow diagram for the automated determination of ferricyanide reductase activity in red blood cells was prepared in the modules from AutoAnalyzer AA I (Technicon Instruments Inc). Ferricyanide reductase assay can be substituted for assay of cytochrome b5 reductase (EC 1.6.2.2), which plays a major role in reducing methaemoglobin in erythrocytes, and is defective specifically in the erythrocytes of patients with hereditary methaemoglobinaemia. The effective sampling rate of the analysis is 30/h, and less than 0.05 ml of whole blood is required. Interference of haemoglobin with absorption by potassium ferricyanide at 420 nm is effectively exculded by dialysis. This automated method was compared with the accepted diaphorase method, and it distinguished clearly the ferricyanide reductase activity of cord bloods from that of adult bloods. The activity of the blood from a patient with hereditary methaemoglobinaemia was only residual. It is suggested that the method is useful as a mass screening test for hereditary methaemoglobinaemia."}
{"id": "PMID:469016", "title": "Electron microscopy of hepatitis B virus components in chronic active liver disease.", "content": "Eighteen liver biopsy specimens from patients with hepatitis B surface antigen (HBsAg) positive chronic aggressive hepatitis were studied by electron microscopy. All cases were selected on the basis of positive liver cell membrane fluorescence for HBsAg on immunohistochemical investigation. Striking changes in the morphology of the liver cell membrane were observed in nearly all cases. Furthermore, a dual aspect of hepatitis B core antigen (HBcAg) is described. HBcAg particles may occur as either 'naked' or 'cloudy' particles surrounded by semi electron dense material. The nature of the 'cloud' remains to be identified.", "contents": "Electron microscopy of hepatitis B virus components in chronic active liver disease. Eighteen liver biopsy specimens from patients with hepatitis B surface antigen (HBsAg) positive chronic aggressive hepatitis were studied by electron microscopy. All cases were selected on the basis of positive liver cell membrane fluorescence for HBsAg on immunohistochemical investigation. Striking changes in the morphology of the liver cell membrane were observed in nearly all cases. Furthermore, a dual aspect of hepatitis B core antigen (HBcAg) is described. HBcAg particles may occur as either 'naked' or 'cloudy' particles surrounded by semi electron dense material. The nature of the 'cloud' remains to be identified."}
{"id": "PMID:469017", "title": "Female adnexal tumour of probable Wolffian origin.", "content": "Two further cases of 'female adnexal tumour of probable Wolffian origin' are described Both were retroperitoneal and presented a unique histology of uniformly close-packed bland spaces. solid islands, cords, and diffuse areas. A small hamartoma in a female fetus, part of which resembled the tumours, was traced to an area of near apposition with some paroophoron canals, providing further evidence of a Wolffian origin. The literature of this and some other putative Wolffian tumours is briefly reviewed, and the relation to rete adenomas is discussed.", "contents": "Female adnexal tumour of probable Wolffian origin. Two further cases of 'female adnexal tumour of probable Wolffian origin' are described Both were retroperitoneal and presented a unique histology of uniformly close-packed bland spaces. solid islands, cords, and diffuse areas. A small hamartoma in a female fetus, part of which resembled the tumours, was traced to an area of near apposition with some paroophoron canals, providing further evidence of a Wolffian origin. The literature of this and some other putative Wolffian tumours is briefly reviewed, and the relation to rete adenomas is discussed."}
{"id": "PMID:469027", "title": "The loci of stuttering: grammar or prosody?", "content": "Much of the research dealing with linguistic dimensions in stuttering has emphasized the various aspects of grammar, particularly as these aspects contribute to the meaning of utterances. However, data from several sources indicate that meaning and grammatical features are not likely to be the important language variables related to stuttering, but that stuttering occurrence is linked more closely to the prosodic dimension. The published literature in this area reports work done with English-speaking subjects; comparable material from other languages would be of considerable value to our understanding of linguistic determinants in stuttering.", "contents": "The loci of stuttering: grammar or prosody? Much of the research dealing with linguistic dimensions in stuttering has emphasized the various aspects of grammar, particularly as these aspects contribute to the meaning of utterances. However, data from several sources indicate that meaning and grammatical features are not likely to be the important language variables related to stuttering, but that stuttering occurrence is linked more closely to the prosodic dimension. The published literature in this area reports work done with English-speaking subjects; comparable material from other languages would be of considerable value to our understanding of linguistic determinants in stuttering."}
{"id": "PMID:469022", "title": "Relative bioavailability of quinidine gluconate and quinidine sulfate in healthy volunteers.", "content": "A comparison of the bioavailability of quinidine sulfate to quinidine gluconate tablets in a single-dose randomized cross-over design with 20 healthy volunteers shows that the sulfate salt is more rapidly absorbed and provides significantly greater peak concentrations 1 hour after administration as compared to the peak levels achieved with the gluconate salt at approximately 5 hours after administration. When adjusted for the actual amount of quinidine contained in each tablet, there was no significant difference in the amount of quinidine bioavailable. Since quinidine gluconate absorption is significantly slower than quinidine sulfate, a combination of the two dosage forms may be utilized in providing the loading dose. Based on the computer modeling and the clinical data accumulated by this laboratory (unpublished) over the past four years, quinidine gluconate, in the dosage form utilized in this study, provides more constant blood levels with smaller differences between the Cpmax and Cpmin than the sulfate when administered every 6 or 8 hours. Further controlled clinical studies are needed to confirm these observations in patients.", "contents": "Relative bioavailability of quinidine gluconate and quinidine sulfate in healthy volunteers. A comparison of the bioavailability of quinidine sulfate to quinidine gluconate tablets in a single-dose randomized cross-over design with 20 healthy volunteers shows that the sulfate salt is more rapidly absorbed and provides significantly greater peak concentrations 1 hour after administration as compared to the peak levels achieved with the gluconate salt at approximately 5 hours after administration. When adjusted for the actual amount of quinidine contained in each tablet, there was no significant difference in the amount of quinidine bioavailable. Since quinidine gluconate absorption is significantly slower than quinidine sulfate, a combination of the two dosage forms may be utilized in providing the loading dose. Based on the computer modeling and the clinical data accumulated by this laboratory (unpublished) over the past four years, quinidine gluconate, in the dosage form utilized in this study, provides more constant blood levels with smaller differences between the Cpmax and Cpmin than the sulfate when administered every 6 or 8 hours. Further controlled clinical studies are needed to confirm these observations in patients."}
{"id": "PMID:469023", "title": "Pharmacokinetics of phenobarbital following single and repeated doses.", "content": "Serum levels of phenobarbital, and also urinary excretion of phenobarbital and p-hydroxyphenobarbital, were examined after single and repeated oral doses of phenobarbital to three male subjects. Serum levels of phenobarbital at steady state were approximately ten times as high as those after a single dose. The overall elimination rate constant for loss of phenobarbital from serum, Kel, was significantly reduced after repeated doses, and Cmax infinity values calculated from single-dose data poorly predicted observed Cmax infinity values. Five-day urinary excretion of phenobarbital and p-hydroxyphenobarbital accounted for 16 and 21 per cent, respectively, of the initial dose. Due to extensive drug accumulation, 83 per cent of the final dose was excreted in five-day urine as phenobarbital and 85 per cent, as p-hydroxyphenobarbital. Comparison of plasma and renal clearances indicated that the rate of phenobarbital metabolism was reduced owing to repeated dosing, while the rate of urinary excretion of parent drug was unchanged.", "contents": "Pharmacokinetics of phenobarbital following single and repeated doses. Serum levels of phenobarbital, and also urinary excretion of phenobarbital and p-hydroxyphenobarbital, were examined after single and repeated oral doses of phenobarbital to three male subjects. Serum levels of phenobarbital at steady state were approximately ten times as high as those after a single dose. The overall elimination rate constant for loss of phenobarbital from serum, Kel, was significantly reduced after repeated doses, and Cmax infinity values calculated from single-dose data poorly predicted observed Cmax infinity values. Five-day urinary excretion of phenobarbital and p-hydroxyphenobarbital accounted for 16 and 21 per cent, respectively, of the initial dose. Due to extensive drug accumulation, 83 per cent of the final dose was excreted in five-day urine as phenobarbital and 85 per cent, as p-hydroxyphenobarbital. Comparison of plasma and renal clearances indicated that the rate of phenobarbital metabolism was reduced owing to repeated dosing, while the rate of urinary excretion of parent drug was unchanged."}
{"id": "PMID:469028", "title": "The use of grammatical morphemes by normal and language-impaired children.", "content": "An examination was made of the grammatical morpheme usage of normal children and language-impaired children matched at two different levels of mean utterance length. The language-impaired children displayed less grammatical morpheme usage than the normal children with equivalent mean utterance length. However, no use of alternative features of semantic importance by the language-impaired children was noted, suggesting that no unique patterns for acquiring language were in operation. Instead, it appears that the language-impaired children may have attached less communicative significance to grammatical morphemes.", "contents": "The use of grammatical morphemes by normal and language-impaired children. An examination was made of the grammatical morpheme usage of normal children and language-impaired children matched at two different levels of mean utterance length. The language-impaired children displayed less grammatical morpheme usage than the normal children with equivalent mean utterance length. However, no use of alternative features of semantic importance by the language-impaired children was noted, suggesting that no unique patterns for acquiring language were in operation. Instead, it appears that the language-impaired children may have attached less communicative significance to grammatical morphemes."}
{"id": "PMID:469029", "title": "Developmental apraxia: diagnostic considerations.", "content": "This study compares the performance of developmentally apraxic children with that of children who have functional articulation disorders on a battery of diagnostic tests. Based on preliminary data, tests which assess auditory attention, auditory discrimination, auditory memory, oral-tactile feedback, and isolated and sequenced volitional oral movements appear to be useful in a differential diagnosis of articulatory defective children and, thus, warrant further study. Implications of these findings are discussed.", "contents": "Developmental apraxia: diagnostic considerations. This study compares the performance of developmentally apraxic children with that of children who have functional articulation disorders on a battery of diagnostic tests. Based on preliminary data, tests which assess auditory attention, auditory discrimination, auditory memory, oral-tactile feedback, and isolated and sequenced volitional oral movements appear to be useful in a differential diagnosis of articulatory defective children and, thus, warrant further study. Implications of these findings are discussed."}
{"id": "PMID:469024", "title": "Interpretation of hematological and biochemical laboratory data in large-scale, multicenter clinical trials.", "content": "The laboratory assessment of drug tolerability is central to a long-term trial. But because of its volume, its multicenter origin, and the importance of nondrug factors, the analysis of these data is complicated. Various methods, i.e., collection of investigators' opinion, comparison of before- and after-treatment means, and analysis of transitions, were found to be unsatisfactory. A fourth method, described in this paper, seems to be more promising. An initial computer screening of the laboratory data is conducted to identify all patients with the potentially clinically relevant laboratory abnormalities. Each laboratory abnormally is then examined by the pharmaceutical physician with regard to the patient's sex and age, the trial diagnosis, concomitant and intercurrent illnesses, concurrent medication, unwanted effects, and other laboratory results, and each result is assigned a probable etiology according to a pre-defined classification system. By this method it is then possible to compare the frequency and severity of possible or probable drug-related laboratory abnormalities occurring with the various trial drugs. Our opinion regarding the importance of using this method would appear justified by the fact that a possible or probable drug effect was considered to have been responsible for only 15 per cent (112/760) of the potentially clinically relevant abnormal tests reported.", "contents": "Interpretation of hematological and biochemical laboratory data in large-scale, multicenter clinical trials. The laboratory assessment of drug tolerability is central to a long-term trial. But because of its volume, its multicenter origin, and the importance of nondrug factors, the analysis of these data is complicated. Various methods, i.e., collection of investigators' opinion, comparison of before- and after-treatment means, and analysis of transitions, were found to be unsatisfactory. A fourth method, described in this paper, seems to be more promising. An initial computer screening of the laboratory data is conducted to identify all patients with the potentially clinically relevant laboratory abnormalities. Each laboratory abnormally is then examined by the pharmaceutical physician with regard to the patient's sex and age, the trial diagnosis, concomitant and intercurrent illnesses, concurrent medication, unwanted effects, and other laboratory results, and each result is assigned a probable etiology according to a pre-defined classification system. By this method it is then possible to compare the frequency and severity of possible or probable drug-related laboratory abnormalities occurring with the various trial drugs. Our opinion regarding the importance of using this method would appear justified by the fact that a possible or probable drug effect was considered to have been responsible for only 15 per cent (112/760) of the potentially clinically relevant abnormal tests reported."}
{"id": "PMID:469030", "title": "Communicative disorders at a health-care center.", "content": "The purpose of this study was to identify the prevalence of communicative disorders in a Children and Youth Program during the 5-yr period 1973-1977. It appears that the numerous speech and hearing surveys recorded fail to do the following: (a) identify speech, language, and hearing pathologie from a comprehensive health-care center and/or hospital; (b) study types of clientele longitudinally; (c) study prevalence and needs of minority groups specifically; and (d) study prevalence of communicative disorders in preshool children. For the Black community under study, it appears that the prevalence of communicative disorders is greater than reported in other surveys. In addition, the current study identifies a community not receiving services. In reviewing the facilities available to this socieconomic community, one finds inadequacies. These inadequacies may be reflected in manpower and equipment rather than programs and resources. The data indicate a tremendous need to expand available services.", "contents": "Communicative disorders at a health-care center. The purpose of this study was to identify the prevalence of communicative disorders in a Children and Youth Program during the 5-yr period 1973-1977. It appears that the numerous speech and hearing surveys recorded fail to do the following: (a) identify speech, language, and hearing pathologie from a comprehensive health-care center and/or hospital; (b) study types of clientele longitudinally; (c) study prevalence and needs of minority groups specifically; and (d) study prevalence of communicative disorders in preshool children. For the Black community under study, it appears that the prevalence of communicative disorders is greater than reported in other surveys. In addition, the current study identifies a community not receiving services. In reviewing the facilities available to this socieconomic community, one finds inadequacies. These inadequacies may be reflected in manpower and equipment rather than programs and resources. The data indicate a tremendous need to expand available services."}
{"id": "PMID:469025", "title": "Bulk laxative efficacy of a psyllium seed hydrocolloid and of a mixture of cellulose and pectin.", "content": "The cellulose/pectin combination (C/P) investigated in this study is as efficacious as a psyllium seed product widely used as a bulk laxative. Because of its physical characteristics (whiteness, lack of flavor and odor, lack of gelling upon standing), it is easy to use as a component of baked foods, sauces, drinks, stews, and in other recipes. Given its fecal bulking equivalence to the popular psyllium-based products, C/P offers advantages to the patient who takes a bulking agent regularly because of the wide choice of methods of consumption, ensuring better compliance over long periods of time. This cellulose/pectin combination appears to be a viable alternative to the limited choice presently available to the bulk laxative user and could also be used as a plant fiber supplement whenever this is desirable.", "contents": "Bulk laxative efficacy of a psyllium seed hydrocolloid and of a mixture of cellulose and pectin. The cellulose/pectin combination (C/P) investigated in this study is as efficacious as a psyllium seed product widely used as a bulk laxative. Because of its physical characteristics (whiteness, lack of flavor and odor, lack of gelling upon standing), it is easy to use as a component of baked foods, sauces, drinks, stews, and in other recipes. Given its fecal bulking equivalence to the popular psyllium-based products, C/P offers advantages to the patient who takes a bulking agent regularly because of the wide choice of methods of consumption, ensuring better compliance over long periods of time. This cellulose/pectin combination appears to be a viable alternative to the limited choice presently available to the bulk laxative user and could also be used as a plant fiber supplement whenever this is desirable."}
{"id": "PMID:469031", "title": "Color perception and deafness: college-level comparisons.", "content": "The performance of three groups of college-level subjects, deaf students, normal-hearing students, and Art students, were compared on nine tests involving color perception and color-verbal materials including (a) Color-Form Sorting, (b) Color-Form Pointing, (c) Color-Word Meaning, (d) Color-Pair Preferences (N=3), and (e) Color-Word Interference (N=3). Color perception differences between the deaf and the hearing groups were not substantiated: deaf and hearing groups differed only on tests involving verbal materials. The Deaf differed more from the Art students than from the Hearing.", "contents": "Color perception and deafness: college-level comparisons. The performance of three groups of college-level subjects, deaf students, normal-hearing students, and Art students, were compared on nine tests involving color perception and color-verbal materials including (a) Color-Form Sorting, (b) Color-Form Pointing, (c) Color-Word Meaning, (d) Color-Pair Preferences (N=3), and (e) Color-Word Interference (N=3). Color perception differences between the deaf and the hearing groups were not substantiated: deaf and hearing groups differed only on tests involving verbal materials. The Deaf differed more from the Art students than from the Hearing."}
{"id": "PMID:469032", "title": "Effects of clinician behaviors on acceptability of patients' responses in aphasia treatment sessions.", "content": "Records of the content of 40 videotaped samples of aphasia treatment sessions were prepared. These records were analyzed to determine (a) whether certain clinician behaviors and task characteristics are related to the occurrence of patient error responses in speech and language treatment sessions for aphasic individuals, and (b) whether \"errors generate errors\"; that is, whether error responses tend to occur in clusters rather than being distributed uniformly throughout the treatment session. The results of these analyses indicated that a number of event categories were significantly related to the occurrence of unacceptable patient responses. These results suggest that certain clinician behaviors generate patient error responses and also that clinicians tend to response to patient errors in characteristic ways. An analysis of these results also confirmed that unacceptable patient responses tended to occur in clusters.", "contents": "Effects of clinician behaviors on acceptability of patients' responses in aphasia treatment sessions. Records of the content of 40 videotaped samples of aphasia treatment sessions were prepared. These records were analyzed to determine (a) whether certain clinician behaviors and task characteristics are related to the occurrence of patient error responses in speech and language treatment sessions for aphasic individuals, and (b) whether \"errors generate errors\"; that is, whether error responses tend to occur in clusters rather than being distributed uniformly throughout the treatment session. The results of these analyses indicated that a number of event categories were significantly related to the occurrence of unacceptable patient responses. These results suggest that certain clinician behaviors generate patient error responses and also that clinicians tend to response to patient errors in characteristic ways. An analysis of these results also confirmed that unacceptable patient responses tended to occur in clusters."}
{"id": "PMID:469033", "title": "An end to anxiety treating speech problems with rhetoritherapy.", "content": "The controversy over the connection between anxiety and nonproductive speech behavior can be resolved by taking note that alteration in behavior usually alters anxiety level. While many theorists recommend that treatment of anxiety will be beneficial, there is little contention that modification of anxiety alone alters skillful behavior. Rhetoritherapy is a mode of changing communication behavior through application of heuristics of problem solving and behavior change. Research studies tend to indicate a high level of success and selected case histories illustrate the process.", "contents": "An end to anxiety treating speech problems with rhetoritherapy. The controversy over the connection between anxiety and nonproductive speech behavior can be resolved by taking note that alteration in behavior usually alters anxiety level. While many theorists recommend that treatment of anxiety will be beneficial, there is little contention that modification of anxiety alone alters skillful behavior. Rhetoritherapy is a mode of changing communication behavior through application of heuristics of problem solving and behavior change. Research studies tend to indicate a high level of success and selected case histories illustrate the process."}
{"id": "PMID:469034", "title": "Wives' evaluation of problems related to laryngectomy.", "content": "Previous questionnaire studies of problems after laryngectomy have been directed toward the patients. In this study, 45 wives of laryngectomees were surveyed regarding their prior understanding of laryngeal surgery. The wives were also asked questions pertaining to health and physical care, and financial and social problems encountered after the operation. The survey results indicate a need for improved family counseling both before and after surgery.", "contents": "Wives' evaluation of problems related to laryngectomy. Previous questionnaire studies of problems after laryngectomy have been directed toward the patients. In this study, 45 wives of laryngectomees were surveyed regarding their prior understanding of laryngeal surgery. The wives were also asked questions pertaining to health and physical care, and financial and social problems encountered after the operation. The survey results indicate a need for improved family counseling both before and after surgery."}
{"id": "PMID:469035", "title": "Residual hypernasality in repaired cleft palate.", "content": "This article reviews selected components of the speech process in children with a repaired cleft palate in order to provide the speech-language pathologist with a framework for decision-making in the selection of appropriate identification and management strategies. Discussion is offered which intends to clarify the phenomena of velopharyngeal valving, nasality, and hypernasality. With an understanding of these fundamental phenomena, suggestions are proposed for management approaches which may be applied to the more common bases of hypernasality in children with repaired clefts.", "contents": "Residual hypernasality in repaired cleft palate. This article reviews selected components of the speech process in children with a repaired cleft palate in order to provide the speech-language pathologist with a framework for decision-making in the selection of appropriate identification and management strategies. Discussion is offered which intends to clarify the phenomena of velopharyngeal valving, nasality, and hypernasality. With an understanding of these fundamental phenomena, suggestions are proposed for management approaches which may be applied to the more common bases of hypernasality in children with repaired clefts."}
{"id": "PMID:469037", "title": "The impact of a maternal and child health care program on the quality of prenatal care: an analysis by risk group.", "content": "A typical maternal and child health care program was implemented in an American Indian reservation community, and its impact on the effectiveness of prenatal care was assessed. Evaluation included examination from both the provider perspective (care provided to program users) and the population perspective (care received by a sample of all prenatal patients in the community), as well as examination of outcomes of prenatal care. The study cohorts were sorted into relatively high and average risk as a function of age, gravidity, and history of abortion or miscarriage. The study results suggest that such a program will improve the quality of prenatal care, although the program has a greater impact on the average risk group. Standard methods of assessing program effectiveness, which examine the care provided by the program as opposed to the care received by the community, will not detect this phenomenon.", "contents": "The impact of a maternal and child health care program on the quality of prenatal care: an analysis by risk group. A typical maternal and child health care program was implemented in an American Indian reservation community, and its impact on the effectiveness of prenatal care was assessed. Evaluation included examination from both the provider perspective (care provided to program users) and the population perspective (care received by a sample of all prenatal patients in the community), as well as examination of outcomes of prenatal care. The study cohorts were sorted into relatively high and average risk as a function of age, gravidity, and history of abortion or miscarriage. The study results suggest that such a program will improve the quality of prenatal care, although the program has a greater impact on the average risk group. Standard methods of assessing program effectiveness, which examine the care provided by the program as opposed to the care received by the community, will not detect this phenomenon."}
{"id": "PMID:469038", "title": "Patients' percentions of physicians' behavior: determinants of patient commitment to the therapeutic relationship.", "content": "This study examines the hypothesis that patients decide to remain committed to their primary care physicians (or to leave them and \"doctor shop\") primarily on the basis of the \"socioemotional\" aspects of the physicians' behavior. It was hypothesized that physicians' demonstrations of caring for patients as people and their openness of communication would be predictive of patient commitment to the therapeutic relationship, and that the physicians' competence and role performance (including thoroughness of information gathering) as perceived by patients would be less important. Interviews were conducted with 342 inpatients and ambulatory patients in a large urban community teaching hospital after a visit with one of 38 house officers in internal medicine. Each patient was asked to (a) rate his or her physician on nine performance variables, (b) indicate his or her intention to return to that physician in the future or seek a different one, and (c) state his or her education, occupation, age, and present illness condition. The physicians' socioemotional behavior (caring and openness to communication), as well as their accessibility, tended to weight heavily in patients' decisions to continue the physician-patient relationship. In addition, the importance of some specific physician behaviors (for example, the physician's willingness to explain the medical condition) varied among subpopulations of patients.", "contents": "Patients' percentions of physicians' behavior: determinants of patient commitment to the therapeutic relationship. This study examines the hypothesis that patients decide to remain committed to their primary care physicians (or to leave them and \"doctor shop\") primarily on the basis of the \"socioemotional\" aspects of the physicians' behavior. It was hypothesized that physicians' demonstrations of caring for patients as people and their openness of communication would be predictive of patient commitment to the therapeutic relationship, and that the physicians' competence and role performance (including thoroughness of information gathering) as perceived by patients would be less important. Interviews were conducted with 342 inpatients and ambulatory patients in a large urban community teaching hospital after a visit with one of 38 house officers in internal medicine. Each patient was asked to (a) rate his or her physician on nine performance variables, (b) indicate his or her intention to return to that physician in the future or seek a different one, and (c) state his or her education, occupation, age, and present illness condition. The physicians' socioemotional behavior (caring and openness to communication), as well as their accessibility, tended to weight heavily in patients' decisions to continue the physician-patient relationship. In addition, the importance of some specific physician behaviors (for example, the physician's willingness to explain the medical condition) varied among subpopulations of patients."}
{"id": "PMID:469039", "title": "The relative emphasis upon physician practice and organizational affairs of a consumer council in a prepaid group practice health plan.", "content": "This paper examines the topics of interest to a consumer advisory council in the Health Insurance Plan of Greater New York, a prepaid group practice. Data are derived from observations of consumer council meetings. Topics considered by the council dealt with (a) health plan services, (b) health plan structure, and (c) consumer council structure, process, and organizational role. The council was primarily interested in retaining and expanding existing services, facilitating utilization of services by enrollees, and achieving broader enrollee representation on the board of directors. These interests are directed toward the organizational context within which care is provided and do not relate to physician-patient encounters. Individual enrollees in prepaid group practice may be concerned with the physician-client relationship but consumer representatives are concerned only with the organization-client relationship.", "contents": "The relative emphasis upon physician practice and organizational affairs of a consumer council in a prepaid group practice health plan. This paper examines the topics of interest to a consumer advisory council in the Health Insurance Plan of Greater New York, a prepaid group practice. Data are derived from observations of consumer council meetings. Topics considered by the council dealt with (a) health plan services, (b) health plan structure, and (c) consumer council structure, process, and organizational role. The council was primarily interested in retaining and expanding existing services, facilitating utilization of services by enrollees, and achieving broader enrollee representation on the board of directors. These interests are directed toward the organizational context within which care is provided and do not relate to physician-patient encounters. Individual enrollees in prepaid group practice may be concerned with the physician-client relationship but consumer representatives are concerned only with the organization-client relationship."}
{"id": "PMID:469040", "title": "Study of the precursors of ovine lactoproteins: primary structures of the 'signals' and enzymic processing of prelactoproteins by mammary microsomal membranes.", "content": "The radiolabelled primary translation products of ovine mammary mRNAs synthesized in a wheat germ cell-free system were isolated by immunoprecipitation and analysed by automated Edman degradation. The 3 'Ca-sensitive' caseins (alpha S1, alpha S2 and beta), kappa-casein, beta-lactoglobulin and alpha-lactalbumin were found to be synthesized as precursors with amino terminal extensions of 15, 21, 18 and 19 amino acid residues respectively. The extra pieces of these various lactoproteins are similar to 'signal' peptides of other secretory proteins in their length and hydrophobicity. The occurrence of an alanyl residue at the C-termini of the extra pieces of the 6 ovine prelactoproteins suggests that the mammary proteinase responsible for the cleavage of the signal peptides may have an elastase-like specificity. When mammary mRNAs were translated in a wheat germ cell-free system in the presence of mammary microsomal membranes, pre-beta-casein was converted into authentic beta-casein as demonstrated by amino terminal sequence analyses. Additionally, pre-beta-casein was post-translationally converted into authentic beta-casein by a specific proteinase(s) extracted from rough microsomes with Na deoxycholate.", "contents": "Study of the precursors of ovine lactoproteins: primary structures of the 'signals' and enzymic processing of prelactoproteins by mammary microsomal membranes. The radiolabelled primary translation products of ovine mammary mRNAs synthesized in a wheat germ cell-free system were isolated by immunoprecipitation and analysed by automated Edman degradation. The 3 'Ca-sensitive' caseins (alpha S1, alpha S2 and beta), kappa-casein, beta-lactoglobulin and alpha-lactalbumin were found to be synthesized as precursors with amino terminal extensions of 15, 21, 18 and 19 amino acid residues respectively. The extra pieces of these various lactoproteins are similar to 'signal' peptides of other secretory proteins in their length and hydrophobicity. The occurrence of an alanyl residue at the C-termini of the extra pieces of the 6 ovine prelactoproteins suggests that the mammary proteinase responsible for the cleavage of the signal peptides may have an elastase-like specificity. When mammary mRNAs were translated in a wheat germ cell-free system in the presence of mammary microsomal membranes, pre-beta-casein was converted into authentic beta-casein as demonstrated by amino terminal sequence analyses. Additionally, pre-beta-casein was post-translationally converted into authentic beta-casein by a specific proteinase(s) extracted from rough microsomes with Na deoxycholate."}
{"id": "PMID:469041", "title": "Role of mammary casein kinase in the phosphorylation of milk proteins.", "content": "Casein kinase from lactating bovine mammary gland catalyses the transfer of the terminal phosphoryl group of ATP to specific serine residues in dephosphorylated caseins. Best substrates for casein kinase are the dephosphorylated proteins (bovine alpha S1- and beta-caseins and pepsin), unphosphorylated human beta-casein and the dephosphorylated peptide (residues 1-25) from bovine beta-casein. Results obtained with bovine and human beta-caseins indicate that the two serines underlined in the cluster Ser-Leu-Ser-Ser-Ser are particularly susceptible to the action of casein kinase. Since a similar sequence is found in dephosphorylated alpha S1-casein, it is probable that serines in this region of alpha S1-casein are also phosphorylated. The results support the concept that certain serines in casein are particularly susceptible to phosphorylation by casein kinase.", "contents": "Role of mammary casein kinase in the phosphorylation of milk proteins. Casein kinase from lactating bovine mammary gland catalyses the transfer of the terminal phosphoryl group of ATP to specific serine residues in dephosphorylated caseins. Best substrates for casein kinase are the dephosphorylated proteins (bovine alpha S1- and beta-caseins and pepsin), unphosphorylated human beta-casein and the dephosphorylated peptide (residues 1-25) from bovine beta-casein. Results obtained with bovine and human beta-caseins indicate that the two serines underlined in the cluster Ser-Leu-Ser-Ser-Ser are particularly susceptible to the action of casein kinase. Since a similar sequence is found in dephosphorylated alpha S1-casein, it is probable that serines in this region of alpha S1-casein are also phosphorylated. The results support the concept that certain serines in casein are particularly susceptible to phosphorylation by casein kinase."}
{"id": "PMID:469042", "title": "The carbohydrate portions of milk glycoproteins.", "content": "kappa-Casein is the main glycoprotein of cow's milk. Its polysaccharide part is O-glycosidically linked to threonine residue 133. It contains only 3 different sugars (Gal, GalNAc, NeuNAc), but a microheterogeneity has been detected at the sugar level. Two main polysaccharides have so far been characterized. The structure of the trisaccharide is NeuNAc alpha 2 leads to 3 Gal beta 1 leads to GalNAc; the tetrasaccharide contains one additional sialic acid. The polysaccharide part of ovine kappa-casein resembles that of bovine kappa-casein, but contains also N-glycolyl neuraminic acid. Human kappa-casein contains 3 times more carbohydrate than bovine kappa-casein with 2 additional sugars, GlcNAc and Fuc. The various polysaccharide parts isolated from bovine colostrum kappa-caseinoglycopeptide are much more complex than those obtained from the normal glycopeptide, indicating an evolution of the sugar part as a function of time after parturition. Some aspects of the secondary structure of kappa-casein and the role of the sugar part are discussed. The carbohydrate moiety of another milk protein, human lactotransferrin, is also discussed briefly. it is comprised of 2 identical glycan groups, N-glycosidically linked to the protein, and quite different from the kappa-casein carbohydrate moiety.", "contents": "The carbohydrate portions of milk glycoproteins. kappa-Casein is the main glycoprotein of cow's milk. Its polysaccharide part is O-glycosidically linked to threonine residue 133. It contains only 3 different sugars (Gal, GalNAc, NeuNAc), but a microheterogeneity has been detected at the sugar level. Two main polysaccharides have so far been characterized. The structure of the trisaccharide is NeuNAc alpha 2 leads to 3 Gal beta 1 leads to GalNAc; the tetrasaccharide contains one additional sialic acid. The polysaccharide part of ovine kappa-casein resembles that of bovine kappa-casein, but contains also N-glycolyl neuraminic acid. Human kappa-casein contains 3 times more carbohydrate than bovine kappa-casein with 2 additional sugars, GlcNAc and Fuc. The various polysaccharide parts isolated from bovine colostrum kappa-caseinoglycopeptide are much more complex than those obtained from the normal glycopeptide, indicating an evolution of the sugar part as a function of time after parturition. Some aspects of the secondary structure of kappa-casein and the role of the sugar part are discussed. The carbohydrate moiety of another milk protein, human lactotransferrin, is also discussed briefly. it is comprised of 2 identical glycan groups, N-glycosidically linked to the protein, and quite different from the kappa-casein carbohydrate moiety."}
{"id": "PMID:469043", "title": "Comparative aspects of milk proteins.", "content": "The current status of knowledge of the major proteins of milks of various species is evaluated. Most of the non-bovine milk proteins are homologous with the recognized families of those of Bos taurus, alpha S1-caseins, alpha S2-caseins, beta-caseins, kappa-caseins, beta-lactoglobulins, and alpha-lactalbumins, each family representing a separate genetic locus specific to the mammary gland. No prominent milk protein not homologous to one of these families has yet been discovered in milk of any species. Genetic polymorphism resulting from substitutions in the polypeptide chains and various degrees of post-translational phosphorylation, glycosylation, and proteolysis have been identified in milk proteins of several species. Total protein production ranges among species from about 0.5 to 10 g/d per kg0.75 maternal weight. Proportions of the several proteins vary greatly among species, but few accurate analytical data are available except for total casein and total whey protein contents.", "contents": "Comparative aspects of milk proteins. The current status of knowledge of the major proteins of milks of various species is evaluated. Most of the non-bovine milk proteins are homologous with the recognized families of those of Bos taurus, alpha S1-caseins, alpha S2-caseins, beta-caseins, kappa-caseins, beta-lactoglobulins, and alpha-lactalbumins, each family representing a separate genetic locus specific to the mammary gland. No prominent milk protein not homologous to one of these families has yet been discovered in milk of any species. Genetic polymorphism resulting from substitutions in the polypeptide chains and various degrees of post-translational phosphorylation, glycosylation, and proteolysis have been identified in milk proteins of several species. Total protein production ranges among species from about 0.5 to 10 g/d per kg0.75 maternal weight. Proportions of the several proteins vary greatly among species, but few accurate analytical data are available except for total casein and total whey protein contents."}
{"id": "PMID:469044", "title": "A genetic and biochemical analysis of a polymorphism of bovine alpha S2-casein.", "content": "Using gel electrophoresis a genetic polymorphism of alpha S2-casein (Cn) was discovered in individual milk samples from 2 bovine breeds of the eastern part of France (Vosgienne and Montb\u00e9liarde). The 3 observed phenotypes (Plate 1) are determined by 2 co-dominant alleles at an autosomal locus. The alpha S2-Cn A variant was the only one known up to now in European breeds (reference variant) and alpha S2-Cn D is a new variant, whose bands overlap the beta-casein A band at pH 8.6, and migrate faster than alpha S2-Cn A at pH 3.0. The sequence of the polypeptide chain alpha S2-Cn D differs from that of alpha S2-Cn A by the deletion of a very acidic nonapeptide, which includes a cluster of 3 phosphoseryl residues. Due to the characteristics of the reference sequence, this deletion cannot be exactly located but it involves residues 50-58, or 51-59, or 52-60. A genetic analysis shows that locus alpha S2-Cn is closely linked to the cluster alpha S1-Cn--beta-Cn--kappa-Cn. The 4 casein species are thus synthesized by 4 closely linked loci.", "contents": "A genetic and biochemical analysis of a polymorphism of bovine alpha S2-casein. Using gel electrophoresis a genetic polymorphism of alpha S2-casein (Cn) was discovered in individual milk samples from 2 bovine breeds of the eastern part of France (Vosgienne and Montb\u00e9liarde). The 3 observed phenotypes (Plate 1) are determined by 2 co-dominant alleles at an autosomal locus. The alpha S2-Cn A variant was the only one known up to now in European breeds (reference variant) and alpha S2-Cn D is a new variant, whose bands overlap the beta-casein A band at pH 8.6, and migrate faster than alpha S2-Cn A at pH 3.0. The sequence of the polypeptide chain alpha S2-Cn D differs from that of alpha S2-Cn A by the deletion of a very acidic nonapeptide, which includes a cluster of 3 phosphoseryl residues. Due to the characteristics of the reference sequence, this deletion cannot be exactly located but it involves residues 50-58, or 51-59, or 52-60. A genetic analysis shows that locus alpha S2-Cn is closely linked to the cluster alpha S1-Cn--beta-Cn--kappa-Cn. The 4 casein species are thus synthesized by 4 closely linked loci."}
{"id": "PMID:469045", "title": "The formation and structure of some proteose-peptone components.", "content": "Two constituents of the proteose-peptone fraction of bovine milk have been isolated and characterized. Component 5 (PP5) has been shown to represent residues 1-105 and 1-107 of the beta-casein amino acid sequence, while component 8-fast (PP8F) corresponds to residues 1-28 of beta-casein. Thus, these proteose-peptones represent the N-terminal portions of the beta-casein molecule, produced by proteolytic cleavages which form the gamma 1, gamma 2 and gamma 3-caseins from the C-terminal part. The continuing formation of the total proteose-peptone fraction, PP5, PP8F and the gamma-caseins during storage of raw milk at 18 or 37 degrees C has been also been demonstrated.", "contents": "The formation and structure of some proteose-peptone components. Two constituents of the proteose-peptone fraction of bovine milk have been isolated and characterized. Component 5 (PP5) has been shown to represent residues 1-105 and 1-107 of the beta-casein amino acid sequence, while component 8-fast (PP8F) corresponds to residues 1-28 of beta-casein. Thus, these proteose-peptones represent the N-terminal portions of the beta-casein molecule, produced by proteolytic cleavages which form the gamma 1, gamma 2 and gamma 3-caseins from the C-terminal part. The continuing formation of the total proteose-peptone fraction, PP5, PP8F and the gamma-caseins during storage of raw milk at 18 or 37 degrees C has been also been demonstrated."}
{"id": "PMID:469046", "title": "The formation of gamma-caseins during cooling of raw milk.", "content": "It has been shown that there is a time-dependent transfer of beta-casein and the milk serine proteinase system from micelles to milk serum with change of temperature from 38 to 4 degrees C. It has been established that the gamma-caseins can be formed by proteolytic degradation of beta-casein. By a simple extraction technique, the very hydrophobic gamma-casein fraction was separated from stored milks (26 and 4 degrees C) and estimated quantitatively. The results showed that the proteolytic degradation of beta-casein is faster at 4 degrees C than at room temperature and this can be explained by the immobilization of enzyme and substrate at the micelle surface at higher temperatures (26 degrees C). The results indicate that irreversible changes during cooling for short periods do not cause problems in milk processing, but the formation of gamma-caseins and phosphopeptides may influence the technological properties of raw milk stored for more than 48 h.", "contents": "The formation of gamma-caseins during cooling of raw milk. It has been shown that there is a time-dependent transfer of beta-casein and the milk serine proteinase system from micelles to milk serum with change of temperature from 38 to 4 degrees C. It has been established that the gamma-caseins can be formed by proteolytic degradation of beta-casein. By a simple extraction technique, the very hydrophobic gamma-casein fraction was separated from stored milks (26 and 4 degrees C) and estimated quantitatively. The results showed that the proteolytic degradation of beta-casein is faster at 4 degrees C than at room temperature and this can be explained by the immobilization of enzyme and substrate at the micelle surface at higher temperatures (26 degrees C). The results indicate that irreversible changes during cooling for short periods do not cause problems in milk processing, but the formation of gamma-caseins and phosphopeptides may influence the technological properties of raw milk stored for more than 48 h."}
{"id": "PMID:469047", "title": "Model proteolysis of beta-casein by immobilized trypsin.", "content": "The enzymic degradation of beta-casein by immobilized trypsin is described and its use as a model system to study proteolytic reactions which occur in milk during processing is examined. Quantitative determination of proteolysis can be obtained by densitometric transmission measurement of patterns obtained by polyacrylamide-gel electrophoresis. Staining properties of some of the individual components in the electrophoretic patterns were determined by calibration with pure protein and peptide preparations. The isolation of pure beta- and gamma-caseins and the preparation of several forms of immobilized trypsin are outlined.", "contents": "Model proteolysis of beta-casein by immobilized trypsin. The enzymic degradation of beta-casein by immobilized trypsin is described and its use as a model system to study proteolytic reactions which occur in milk during processing is examined. Quantitative determination of proteolysis can be obtained by densitometric transmission measurement of patterns obtained by polyacrylamide-gel electrophoresis. Staining properties of some of the individual components in the electrophoretic patterns were determined by calibration with pure protein and peptide preparations. The isolation of pure beta- and gamma-caseins and the preparation of several forms of immobilized trypsin are outlined."}
{"id": "PMID:469048", "title": "Proteolytic activity in UHT-sterilized milk.", "content": "The proteolytic activity in UHT-sterilized milk of a proteinase-containing fraction, prepared from the milk, is reported. The proteinase fraction was isolated from casein micelles as described by Reimerdes & Klostermeyer (1974). The fraction was added to UHT milks and the proteolysis in the samples, measured as the liberation of non-protein nitrogen, compared with that in control samples without added proteinases. The increase in proteolytic activity and deterioration in samples containing added proteinases indicates that an enzyme process occurred in UHT-sterilized milk which could influence gel formation.", "contents": "Proteolytic activity in UHT-sterilized milk. The proteolytic activity in UHT-sterilized milk of a proteinase-containing fraction, prepared from the milk, is reported. The proteinase fraction was isolated from casein micelles as described by Reimerdes & Klostermeyer (1974). The fraction was added to UHT milks and the proteolysis in the samples, measured as the liberation of non-protein nitrogen, compared with that in control samples without added proteinases. The increase in proteolytic activity and deterioration in samples containing added proteinases indicates that an enzyme process occurred in UHT-sterilized milk which could influence gel formation."}
{"id": "PMID:469049", "title": "Buffalo alpha S0-casein: isolation and characterization.", "content": "A method for the preparation of alpha S0-casein, and for the preparation of alpha S1-casein fractions 1 and 2 from buffalo casein are described. The amino acid composition and phosphorus content of alpha S0-casein are given and compared with those of other alphaS-caseins.", "contents": "Buffalo alpha S0-casein: isolation and characterization. A method for the preparation of alpha S0-casein, and for the preparation of alpha S1-casein fractions 1 and 2 from buffalo casein are described. The amino acid composition and phosphorus content of alpha S0-casein are given and compared with those of other alphaS-caseins."}
{"id": "PMID:469050", "title": "Human beta-casein.", "content": "Human beta-casein occurs in multiphosphorylated forms having the same amino acid composition but with 0-5 phosphate groups/molecule. Sequence analysis was used to determine whether each of the phosphorylated forms is a mixture of species having a certain number of phosphate groups randomly distributed or whether each form contains phosphate groups on specific seryl or threonyl residues. It was found that forms containing 2, 4 and 5 phosphate groups/molecule are homogeneous with respect to their phosphorylation sites. The monophosphorylated form, however, is a mixture of equal amounts of species phosphorylated at residues 9 or 10.", "contents": "Human beta-casein. Human beta-casein occurs in multiphosphorylated forms having the same amino acid composition but with 0-5 phosphate groups/molecule. Sequence analysis was used to determine whether each of the phosphorylated forms is a mixture of species having a certain number of phosphate groups randomly distributed or whether each form contains phosphate groups on specific seryl or threonyl residues. It was found that forms containing 2, 4 and 5 phosphate groups/molecule are homogeneous with respect to their phosphorylation sites. The monophosphorylated form, however, is a mixture of equal amounts of species phosphorylated at residues 9 or 10."}
{"id": "PMID:469051", "title": "Association of proteins.", "content": "A review is presented of the various intermolecular forces important in stabilizing protein structure and association. Emphasis is put on entropic as well as enthalpic contributions to the free energy. The factors affecting size and size distribution in protein aggregates are discussed. A theory is presented for the size distribution of casein micelles, taking into account the spherical nature of the micelles and the importance of surface repulsive forces in limiting size.", "contents": "Association of proteins. A review is presented of the various intermolecular forces important in stabilizing protein structure and association. Emphasis is put on entropic as well as enthalpic contributions to the free energy. The factors affecting size and size distribution in protein aggregates are discussed. A theory is presented for the size distribution of casein micelles, taking into account the spherical nature of the micelles and the importance of surface repulsive forces in limiting size."}
{"id": "PMID:469053", "title": "Quantitation of alpha S1-casein aggregation by the use of polyfunctional models.", "content": "A model for the coagulation of Ca2+ alpha S1- caseinate is described wherein the casein molecules are considered to aggregate as polyfunctional units. The functionalities on the protein molecules are produced as a consequence of the binding of Ca2+, and the multiple equilibria which are established during this binding produce a distribution in which not all casein molecules have the same functionality. The theory is compared with experimental data of the precipitation reaction, and is shown to provide an adequate quantitative description of the precipitation.", "contents": "Quantitation of alpha S1-casein aggregation by the use of polyfunctional models. A model for the coagulation of Ca2+ alpha S1- caseinate is described wherein the casein molecules are considered to aggregate as polyfunctional units. The functionalities on the protein molecules are produced as a consequence of the binding of Ca2+, and the multiple equilibria which are established during this binding produce a distribution in which not all casein molecules have the same functionality. The theory is compared with experimental data of the precipitation reaction, and is shown to provide an adequate quantitative description of the precipitation."}
{"id": "PMID:469054", "title": "The kinetics of the precipitation of chemically modified alpha S1-casein by calcium.", "content": "The aggregation of alpha S1-casein by Ca is viewed as resulting from a reduction in the net negative charge by the binding of positively charged Ca2+. This reduction in charge leads to the equivalent of isoelectric precipitation. A quantitative relationship between the rate of aggregation of the casein and the monomer net charge is predicted in this isoelectric precipitation model. Precipitation  results are presented for alpha S1-casein labelled with dansyl chloride. Providing the changes in charge brought about by the modification are taken into account, the precipitation behaviour of these caseins can be reconciled with that of native alpha S1-casein. This emphasises the role of electrostatic repulsion in this process and lend further support to the isoelectric precipitation model.", "contents": "The kinetics of the precipitation of chemically modified alpha S1-casein by calcium. The aggregation of alpha S1-casein by Ca is viewed as resulting from a reduction in the net negative charge by the binding of positively charged Ca2+. This reduction in charge leads to the equivalent of isoelectric precipitation. A quantitative relationship between the rate of aggregation of the casein and the monomer net charge is predicted in this isoelectric precipitation model. Precipitation  results are presented for alpha S1-casein labelled with dansyl chloride. Providing the changes in charge brought about by the modification are taken into account, the precipitation behaviour of these caseins can be reconciled with that of native alpha S1-casein. This emphasises the role of electrostatic repulsion in this process and lend further support to the isoelectric precipitation model."}
{"id": "PMID:469055", "title": "On the size of monomers and polymers of beta-casein.", "content": "The size and number average molecular weight have been determined for beta-casein monomers and polymers from electron micrographs using the freeze-etching procedure with spray-frozen specimens. For the spherical beta-casein monomers we found a mol. wt of 22600 and a diam. about 10 nm, which compared quite well with data obtained from ultracentrifugation, light scattering and viscosity measurements. Polymer sizes were in agreement with molecular weight determinations from ultracentrifugation and light scattering, assuming that the volume and weight of the particles are proportional.", "contents": "On the size of monomers and polymers of beta-casein. The size and number average molecular weight have been determined for beta-casein monomers and polymers from electron micrographs using the freeze-etching procedure with spray-frozen specimens. For the spherical beta-casein monomers we found a mol. wt of 22600 and a diam. about 10 nm, which compared quite well with data obtained from ultracentrifugation, light scattering and viscosity measurements. Polymer sizes were in agreement with molecular weight determinations from ultracentrifugation and light scattering, assuming that the volume and weight of the particles are proportional."}
{"id": "PMID:469056", "title": "Structure of beta-casein.", "content": "The temperature and concentration dependent association of beta-casein was studied by means of viscometry, gel filtration chromatography, electron microscopy, analytical ultracentrifugation and UV difference spectrophotometry. Degrees of polymerization of 12, 22 and 49 and free energies of association of -21, -23 and -25kJ/mole monomer were found at temperatures of 10, 15 and 20 degrees C respectively in 0.2 M Na phosphate buffer pH 6.7. Monomeric beta-casein was not a completely random coil but became more compact with increasing temperature, due to hydrophobic interactions.", "contents": "Structure of beta-casein. The temperature and concentration dependent association of beta-casein was studied by means of viscometry, gel filtration chromatography, electron microscopy, analytical ultracentrifugation and UV difference spectrophotometry. Degrees of polymerization of 12, 22 and 49 and free energies of association of -21, -23 and -25kJ/mole monomer were found at temperatures of 10, 15 and 20 degrees C respectively in 0.2 M Na phosphate buffer pH 6.7. Monomeric beta-casein was not a completely random coil but became more compact with increasing temperature, due to hydrophobic interactions."}
{"id": "PMID:469057", "title": "Casein micelles: the colloid-chemical approach.", "content": "The colloidal properties of micellar casein are reviewed. It is shown that behaviour of intact micelles is much at variance with the predictions from the Schulze-Hardy rule, and that therefore their stability cannot be explained by the principles of the DLVO theory. Towards electrolyte, micelles behave as a protein rather than a lyophobic colloid. Casein is a strong protective colloid. In the micelle, however, it does not completely cover the inorganic constituent which remains sensitive to changes in the ionic environment. The rate theory of the enzyme-induced clotting of casein micelles is summarized. It is shown that the lag phase in the clotting is due to the second order of the co-agulation reaction. Flocculation rate constants of micelles have been deduced from clotting times. Their relatively low values can be attributed to an orientational constraint. Practical consequences of the theory with respect to clot structure, gelation of sterilized products and cheese manufacture are discussed.", "contents": "Casein micelles: the colloid-chemical approach. The colloidal properties of micellar casein are reviewed. It is shown that behaviour of intact micelles is much at variance with the predictions from the Schulze-Hardy rule, and that therefore their stability cannot be explained by the principles of the DLVO theory. Towards electrolyte, micelles behave as a protein rather than a lyophobic colloid. Casein is a strong protective colloid. In the micelle, however, it does not completely cover the inorganic constituent which remains sensitive to changes in the ionic environment. The rate theory of the enzyme-induced clotting of casein micelles is summarized. It is shown that the lag phase in the clotting is due to the second order of the co-agulation reaction. Flocculation rate constants of micelles have been deduced from clotting times. Their relatively low values can be attributed to an orientational constraint. Practical consequences of the theory with respect to clot structure, gelation of sterilized products and cheese manufacture are discussed."}
{"id": "PMID:469058", "title": "Developments in column chromatography for the separation and characterization of casein micelles.", "content": "Skim-milk was fractionated by permeation chromatography on CPG-10 (300 nm) at 20 degrees C with synthetic milk serum. The elution profile, which was highly reproducible, showed 3 partly resolved peaks. On treatment with 0.1% glutaraldehyde before chromatography, a similar profile was obtained indicating no appreciable micellar dissociation during chromatography of the milk. The casein composition of the eluent fractions, determined by quantitative chromatography on hydroxyapatite, showed that 12.1% of total casein was associated with the low molecular weight peak. The micelle content of beta- and kappa-casein increased with increasing elution volume, and the alphas/kappa ratio showed a progressive diminution from 4.3 to 2.3. Large differences were found in the colloidal phosphate contents and the weight average diameters of the micelle fractions which clearly show that CPG-10 fractionates on the basis of molecular size.", "contents": "Developments in column chromatography for the separation and characterization of casein micelles. Skim-milk was fractionated by permeation chromatography on CPG-10 (300 nm) at 20 degrees C with synthetic milk serum. The elution profile, which was highly reproducible, showed 3 partly resolved peaks. On treatment with 0.1% glutaraldehyde before chromatography, a similar profile was obtained indicating no appreciable micellar dissociation during chromatography of the milk. The casein composition of the eluent fractions, determined by quantitative chromatography on hydroxyapatite, showed that 12.1% of total casein was associated with the low molecular weight peak. The micelle content of beta- and kappa-casein increased with increasing elution volume, and the alphas/kappa ratio showed a progressive diminution from 4.3 to 2.3. Large differences were found in the colloidal phosphate contents and the weight average diameters of the micelle fractions which clearly show that CPG-10 fractionates on the basis of molecular size."}
{"id": "PMID:469059", "title": "CPG-chromatography studies of the stability of casein micelles.", "content": "Casein micelles fractionated on controlled pore glass (CPG-10/3000) were shown to be stable by recycling experiments. Only minor effects on the size distribution of the casein micelles were found after heating skim-milk to 100 degrees C for 10 min, or freeze-drying skim-milk at -70 degrees C followed by resuspension in the synthetic milk serum of Jenness & Koops (1962). The heating caused some whey proteins (beta-lactoglobulin) to enter the micelle fractions while the freeze-drying caused some of the largest micelles to disrupt. In colloidal calcium phosphate-free skim-milk prepared according to Pyne & McGann (1960) all the micelles appeared to dissociate into monomeric caseins.", "contents": "CPG-chromatography studies of the stability of casein micelles. Casein micelles fractionated on controlled pore glass (CPG-10/3000) were shown to be stable by recycling experiments. Only minor effects on the size distribution of the casein micelles were found after heating skim-milk to 100 degrees C for 10 min, or freeze-drying skim-milk at -70 degrees C followed by resuspension in the synthetic milk serum of Jenness & Koops (1962). The heating caused some whey proteins (beta-lactoglobulin) to enter the micelle fractions while the freeze-drying caused some of the largest micelles to disrupt. In colloidal calcium phosphate-free skim-milk prepared according to Pyne & McGann (1960) all the micelles appeared to dissociate into monomeric caseins."}
{"id": "PMID:469060", "title": "The voluminosity of bovine casein micelles and some of its implications.", "content": "A comparison of literature results on the voluminosity of casein micelles yields large differences, largely related to the method of determination. After applying some corrections, methods based on hydrodynamic radius yield a value of roughly 3.9 ml/g dry casein, other methods (microscopy, sediment volume) about 2.2. Roughly half of the discrepancy can be explained by the micelles being not perfectly spherical. To explain the remaining difference, it is assumed that the micelles are hairy, i.e. they have molecular chains protruding into the milk serum. The hairyness would increase with decreasing temperature and be largely removed by the action of rennet. It would cause some entropic repulsion between micelles at close approach.", "contents": "The voluminosity of bovine casein micelles and some of its implications. A comparison of literature results on the voluminosity of casein micelles yields large differences, largely related to the method of determination. After applying some corrections, methods based on hydrodynamic radius yield a value of roughly 3.9 ml/g dry casein, other methods (microscopy, sediment volume) about 2.2. Roughly half of the discrepancy can be explained by the micelles being not perfectly spherical. To explain the remaining difference, it is assumed that the micelles are hairy, i.e. they have molecular chains protruding into the milk serum. The hairyness would increase with decreasing temperature and be largely removed by the action of rennet. It would cause some entropic repulsion between micelles at close approach."}
{"id": "PMID:469061", "title": "Hydration of casein micelles: kinetics and isotherms of water sorption of micellar casein isolated from fresh and heat-treated milk.", "content": "Water vapour sorption isotherms of casein micelles prepared from raw milk and various heat-treated milks were determined. The equilibrium water contents of the heated preparations were markedly lower than that of the raw-milk casein over the whole range of vapour pressures studied. An analysis of the sorption isotherms in the relative vapour pressure range 0.1--0.45, according to the Brunauer, Emmett & Teller (1938) equation, showed that there were significant differences between preparations in the computed monolayer contents. Differences in the rates of water sorption were also observed between the different preparations. As judged from the amount of absorbed water, the influence of the heating methods could be ranked in the order: HTST (92 degrees C) approximately UHT (direct) less than UHT (indirect) less than HTST (72 degrees C).", "contents": "Hydration of casein micelles: kinetics and isotherms of water sorption of micellar casein isolated from fresh and heat-treated milk. Water vapour sorption isotherms of casein micelles prepared from raw milk and various heat-treated milks were determined. The equilibrium water contents of the heated preparations were markedly lower than that of the raw-milk casein over the whole range of vapour pressures studied. An analysis of the sorption isotherms in the relative vapour pressure range 0.1--0.45, according to the Brunauer, Emmett & Teller (1938) equation, showed that there were significant differences between preparations in the computed monolayer contents. Differences in the rates of water sorption were also observed between the different preparations. As judged from the amount of absorbed water, the influence of the heating methods could be ranked in the order: HTST (92 degrees C) approximately UHT (direct) less than UHT (indirect) less than HTST (72 degrees C)."}
{"id": "PMID:469062", "title": "The role of calcium, phosphate and citrate ions in the stabilization of casein micelles.", "content": "To obtain greater insight into the interaction of Ca, citrate and phosphate ions with casein, 31PNMR measurements were performed on combinations of these ions with alphas- and kappa-caseins. It was found that addition of alphas-casein to a Ca phosphate solution in D2O at 27 degrees C and pD 6.4 resulted in a downfield shift of the 31P singlet. An almost identical shift was observed with kappa-casein, but no shift was found when only phosphate ions were present or when Ca2+ were added to phosphate ions in the absence of casein. Separate experiments with poly-L-lysine, mol. wt approx. 35,000, resulted in similar downfield 31P chemical shifts of Ca phosphate as with both caseins, whereas no shift was observed when poly-glycine was added. From these results it can be concluded that Ca and phosphate ions associate with casein in a co-operative manner, probably in the way described by ter Horst (1963) as a complex with the NH3+-groups of lysine or arginine in a structure such as: (casein--NH3+)--PO43---Ca2+. The formation of this complex may be enhanced by citrate ions, since preliminary results have shown that addition of Na citrate to a solution of alphas-casein with added Ca phosphate produces a broadening of the 31P signal as well as a chemical shift.", "contents": "The role of calcium, phosphate and citrate ions in the stabilization of casein micelles. To obtain greater insight into the interaction of Ca, citrate and phosphate ions with casein, 31PNMR measurements were performed on combinations of these ions with alphas- and kappa-caseins. It was found that addition of alphas-casein to a Ca phosphate solution in D2O at 27 degrees C and pD 6.4 resulted in a downfield shift of the 31P singlet. An almost identical shift was observed with kappa-casein, but no shift was found when only phosphate ions were present or when Ca2+ were added to phosphate ions in the absence of casein. Separate experiments with poly-L-lysine, mol. wt approx. 35,000, resulted in similar downfield 31P chemical shifts of Ca phosphate as with both caseins, whereas no shift was observed when poly-glycine was added. From these results it can be concluded that Ca and phosphate ions associate with casein in a co-operative manner, probably in the way described by ter Horst (1963) as a complex with the NH3+-groups of lysine or arginine in a structure such as: (casein--NH3+)--PO43---Ca2+. The formation of this complex may be enhanced by citrate ions, since preliminary results have shown that addition of Na citrate to a solution of alphas-casein with added Ca phosphate produces a broadening of the 31P signal as well as a chemical shift."}
{"id": "PMID:469063", "title": "The binding of beta-casein to hydroxyapatite: the effect of phosphate content and location.", "content": "The elution behaviour of beta-casein from columns of hydroxyapatite has been studied and the effect examined of the enzymic addition of an extra phosphate residue. When the additional phosphate is located near pre-existing phosphate residues stronger binding to hydroxyapatite is observed. When the additional phosphate is remote from the pre-existing phosphates no increase in strength of binding is observed. It is suggested that the clustering of phosphate residues which characterizes alphas- and beta-caseins can be rationalized on the basis that it facilitates co-operative interactions between these phosphates and the Ca phosphate of the casein micelle and/or basic residues within casein polypeptide chains.", "contents": "The binding of beta-casein to hydroxyapatite: the effect of phosphate content and location. The elution behaviour of beta-casein from columns of hydroxyapatite has been studied and the effect examined of the enzymic addition of an extra phosphate residue. When the additional phosphate is located near pre-existing phosphate residues stronger binding to hydroxyapatite is observed. When the additional phosphate is remote from the pre-existing phosphates no increase in strength of binding is observed. It is suggested that the clustering of phosphate residues which characterizes alphas- and beta-caseins can be rationalized on the basis that it facilitates co-operative interactions between these phosphates and the Ca phosphate of the casein micelle and/or basic residues within casein polypeptide chains."}
{"id": "PMID:469064", "title": "Sub-structure of synthetic casein micelles.", "content": "Casein micelles of different composition were synthesized in various ways and their sub-structure was investigated with the electron microscope by means of thin sections. Earlier studies of Schmidt et al. (1974) using the freeze-fracturing technique had shown no differences in the sub-structure of natural micelles and artificial micelles containing Ca and casein only. Contrary to these results our present studies showed that for the production of synthetic casein micelles with the same sub-structure as the natural ones it is necessary to add at least 2 other ions to the casein solution besides Ca2+: these are phosphate and citrate. The citrate ions play an important role in forming the material of the dark framework of the micelles visible in electron micrographs of unstained thin sections. This supports the hypothesis of Pyne & McGann (1960) that casein micelles contain a citrate apatite.", "contents": "Sub-structure of synthetic casein micelles. Casein micelles of different composition were synthesized in various ways and their sub-structure was investigated with the electron microscope by means of thin sections. Earlier studies of Schmidt et al. (1974) using the freeze-fracturing technique had shown no differences in the sub-structure of natural micelles and artificial micelles containing Ca and casein only. Contrary to these results our present studies showed that for the production of synthetic casein micelles with the same sub-structure as the natural ones it is necessary to add at least 2 other ions to the casein solution besides Ca2+: these are phosphate and citrate. The citrate ions play an important role in forming the material of the dark framework of the micelles visible in electron micrographs of unstained thin sections. This supports the hypothesis of Pyne & McGann (1960) that casein micelles contain a citrate apatite."}
{"id": "PMID:469065", "title": "Properties of artificial casein micelles.", "content": "A survey is given of the relationships between various properties of artificial casein micelle systems and their composition with respect to alphas1-, beta and kappa-casein, colloidal phosphate and citrate. Properties investigated were: the amount of colloidal phosphate, the micellar size, and the stability of the micelle towards dialysis, pressure, ethanol and heat.", "contents": "Properties of artificial casein micelles. A survey is given of the relationships between various properties of artificial casein micelle systems and their composition with respect to alphas1-, beta and kappa-casein, colloidal phosphate and citrate. Properties investigated were: the amount of colloidal phosphate, the micellar size, and the stability of the micelle towards dialysis, pressure, ethanol and heat."}
{"id": "PMID:469066", "title": "Heat stability in concentrated and non-concentrated milks--the effect of urea and beta-lactoglobulin levels and the influence of preheating.", "content": "Heat stability as a function of pH has been studied in concentrated and non-concentrated milk systems. The influence of preheating has also been examined. The concentration of beta-lactoglobulin has been shown to affect markedly the heat stability behaviour in both systems but with different characteristics. Increasing the level of urea resulted in increased heat stability in non-concentrated milks, but corresponding concentrated milks showed a small decrease in maximum heat stability. It has not been possible to extrapolate heat stability data from non-concentrated to concentrated milks.", "contents": "Heat stability in concentrated and non-concentrated milks--the effect of urea and beta-lactoglobulin levels and the influence of preheating. Heat stability as a function of pH has been studied in concentrated and non-concentrated milk systems. The influence of preheating has also been examined. The concentration of beta-lactoglobulin has been shown to affect markedly the heat stability behaviour in both systems but with different characteristics. Increasing the level of urea resulted in increased heat stability in non-concentrated milks, but corresponding concentrated milks showed a small decrease in maximum heat stability. It has not been possible to extrapolate heat stability data from non-concentrated to concentrated milks."}
{"id": "PMID:469067", "title": "Stability of buffalo casein micelles.", "content": "Buffalo skim-milk is less heat stable than cow skim-milk. Interchanging ultracentrifugal whey (UCW) and milk diffusate with micellar casein caused significant changes in the heat stability of buffalo casein micelles (BCM) and cow casein micelles (CCM). Buffalo UCW dramatically destabilized CCM, whereas buffalo diffusate with CCM exhibited the highest heat stability. Cow kappa-casein stabilizes alphas-casein against precipitation by Ca better than buffalo kappa-casein. About 90% of alphas-casein could be stabilized by kappa:alphas ratios of 0.20 and 0.231 for cow and buffalo, respectively. Sialic acid release from micellar kappa-casein by rennet was higher than from acid kappa-casein in both buffalo and cow caseins, the release being slower in buffalo. The released macropeptide from buffalo kappa-casein was smaller than that from cow kappa-casein as revealed by Sephadex gel filtration. Sub-units of BCM have less sialic acid (1.57 mg/g) than whole micelles (2.70 mg/g). On rennet action, 47% of bound sialic acid was released from sub-units as against 85% from whole micelles. The sub-micelles are less heat stable than whole micelles. Among ions tested, added Ca reduced heat stability more dramatically in whole micelles, whereas added phosphate improved the stability of micelles and, more strikingly, of sub-micelles. Citrate also improved the heat stability of sub-micelles but not of whole micelles.", "contents": "Stability of buffalo casein micelles. Buffalo skim-milk is less heat stable than cow skim-milk. Interchanging ultracentrifugal whey (UCW) and milk diffusate with micellar casein caused significant changes in the heat stability of buffalo casein micelles (BCM) and cow casein micelles (CCM). Buffalo UCW dramatically destabilized CCM, whereas buffalo diffusate with CCM exhibited the highest heat stability. Cow kappa-casein stabilizes alphas-casein against precipitation by Ca better than buffalo kappa-casein. About 90% of alphas-casein could be stabilized by kappa:alphas ratios of 0.20 and 0.231 for cow and buffalo, respectively. Sialic acid release from micellar kappa-casein by rennet was higher than from acid kappa-casein in both buffalo and cow caseins, the release being slower in buffalo. The released macropeptide from buffalo kappa-casein was smaller than that from cow kappa-casein as revealed by Sephadex gel filtration. Sub-units of BCM have less sialic acid (1.57 mg/g) than whole micelles (2.70 mg/g). On rennet action, 47% of bound sialic acid was released from sub-units as against 85% from whole micelles. The sub-micelles are less heat stable than whole micelles. Among ions tested, added Ca reduced heat stability more dramatically in whole micelles, whereas added phosphate improved the stability of micelles and, more strikingly, of sub-micelles. Citrate also improved the heat stability of sub-micelles but not of whole micelles."}
{"id": "PMID:469101", "title": "Peritoneal dialysis clearance of endogenous lactate.", "content": "Four patients with severe lactic acidosis were treated with peritoneal dialysis using a bicarbonate-buffered dialysate. Peritoneal dialysis clearance of lactate averaged 9.1 ml minute, and the total lactate removed during dialysis ranged between 88 and 352 mEq. Removal of excess lactate by dialysis contributes to the control of acidosis and prevention of alkalosis once the metabolic defect is reversed.", "contents": "Peritoneal dialysis clearance of endogenous lactate. Four patients with severe lactic acidosis were treated with peritoneal dialysis using a bicarbonate-buffered dialysate. Peritoneal dialysis clearance of lactate averaged 9.1 ml minute, and the total lactate removed during dialysis ranged between 88 and 352 mEq. Removal of excess lactate by dialysis contributes to the control of acidosis and prevention of alkalosis once the metabolic defect is reversed."}
{"id": "PMID:469102", "title": "Standards for accurate in vitro characterization of dialyzers: test case the Vivacell.", "content": "A standard format is proposed for cataloging dialyzer transport data in order to improve communication and understanding of published results. The need for such a format is obvious from the ambiguity and lack of uniformity in published data. This report for in vitro characterization also describes an adequate experimental set-up for proper dialyzer characterization and an improved means for reporting uncertainty where curve fitting techniques are employed. The Vivacell dialyzer has been evaluated by these means as the first test case.", "contents": "Standards for accurate in vitro characterization of dialyzers: test case the Vivacell. A standard format is proposed for cataloging dialyzer transport data in order to improve communication and understanding of published results. The need for such a format is obvious from the ambiguity and lack of uniformity in published data. This report for in vitro characterization also describes an adequate experimental set-up for proper dialyzer characterization and an improved means for reporting uncertainty where curve fitting techniques are employed. The Vivacell dialyzer has been evaluated by these means as the first test case."}
{"id": "PMID:469103", "title": "The use of behavior modification techniques for the treatment of hemodialysis patient non-compliance: a case study.", "content": "This study concerns a renal patient who was non-compliant to staff requests to monitor his blood clotting times. The non-compliant behavior was resolved within six treatment sessions through the combined use of contracting, shaping, stimulus control, discrimination training and social and material reinforcement. Follow-ups at 30, 60 and 90 days revealed a high degree of patient compliance and accuracy in taking and recording his blood clotting times. Implications for behavior modification techniques in the field of hemodialysis are briefly discussed.", "contents": "The use of behavior modification techniques for the treatment of hemodialysis patient non-compliance: a case study. This study concerns a renal patient who was non-compliant to staff requests to monitor his blood clotting times. The non-compliant behavior was resolved within six treatment sessions through the combined use of contracting, shaping, stimulus control, discrimination training and social and material reinforcement. Follow-ups at 30, 60 and 90 days revealed a high degree of patient compliance and accuracy in taking and recording his blood clotting times. Implications for behavior modification techniques in the field of hemodialysis are briefly discussed."}
{"id": "PMID:469104", "title": "Dialysis of middle molecules at pulsatile flow.", "content": "Pulsatile flow was used in a continuous flow flat-plate dialyzer in order to achieve the more efficient removal of middle molecules. The solutes ranged in the molecular weights from 342 (sucrose) to 1355 (vitamin B-12). The permeability of Cuprophane membrane to sucrose, raffinose and vitamin B-12 were calculated both for pulseless and pulsatile flows. It was found that pulsatile flow is more favorable than steady flow with regard to increase of membrane permeability. However, as the solute molecular weight increases, fluid-phase resistances become relatively less important and the transport rate becomes dominated by the membrane properties.", "contents": "Dialysis of middle molecules at pulsatile flow. Pulsatile flow was used in a continuous flow flat-plate dialyzer in order to achieve the more efficient removal of middle molecules. The solutes ranged in the molecular weights from 342 (sucrose) to 1355 (vitamin B-12). The permeability of Cuprophane membrane to sucrose, raffinose and vitamin B-12 were calculated both for pulseless and pulsatile flows. It was found that pulsatile flow is more favorable than steady flow with regard to increase of membrane permeability. However, as the solute molecular weight increases, fluid-phase resistances become relatively less important and the transport rate becomes dominated by the membrane properties."}
{"id": "PMID:469105", "title": "Hemodialysis for methanol intoxication.", "content": "A patient with toxic levels of methanol was hemodialyzed while receiving an ethanol infusion. It was demonstrated that during the dialysis significant quantities of both ethanol (17.70 gms) and methanol (26.69 gms) were extracted. Also, it was shown that the ethanol did not competetively inhibit the extraction of the methanol. Since ethanol is removed in significant quantities during dialysis, one should increase its infusion rate during hemodialysis and decrease the rate when the patient is not being dialyzed. This would achieve adequate and constant blood levels of ethanol and would satisfactorily inhibit the metabolism of methanol by the liver.", "contents": "Hemodialysis for methanol intoxication. A patient with toxic levels of methanol was hemodialyzed while receiving an ethanol infusion. It was demonstrated that during the dialysis significant quantities of both ethanol (17.70 gms) and methanol (26.69 gms) were extracted. Also, it was shown that the ethanol did not competetively inhibit the extraction of the methanol. Since ethanol is removed in significant quantities during dialysis, one should increase its infusion rate during hemodialysis and decrease the rate when the patient is not being dialyzed. This would achieve adequate and constant blood levels of ethanol and would satisfactorily inhibit the metabolism of methanol by the liver."}
{"id": "PMID:469106", "title": "The work of worrying in children undergoing surgery.", "content": "This study examined the relationship involving children's level of anxiety, defensiveness, and play patterns 1 week prior to, during, and 1 week after hospitalization for minor surgery. A 7-month follow-up was also conducted in order to assess the children's recall for hospital events and coping style. The results suggested that two classes of children could be identified. The children in one group distinguished themselves in terms of their disposition to engage in the \"work of worrying\" (i.e., were low defensive prior to hospitalization, actively played with stress-related toys prior to hospitalization, and reported minimal distress and anxiety following surgery). Those in the second group were highly defensive, avoided playing with stress-related toys, and reported most anxiety following surgery. The parallel between children and adults patterns of dealing with surgery was considered in terms of Janis's (1978) model of coping with psychological stress.", "contents": "The work of worrying in children undergoing surgery. This study examined the relationship involving children's level of anxiety, defensiveness, and play patterns 1 week prior to, during, and 1 week after hospitalization for minor surgery. A 7-month follow-up was also conducted in order to assess the children's recall for hospital events and coping style. The results suggested that two classes of children could be identified. The children in one group distinguished themselves in terms of their disposition to engage in the \"work of worrying\" (i.e., were low defensive prior to hospitalization, actively played with stress-related toys prior to hospitalization, and reported minimal distress and anxiety following surgery). Those in the second group were highly defensive, avoided playing with stress-related toys, and reported most anxiety following surgery. The parallel between children and adults patterns of dealing with surgery was considered in terms of Janis's (1978) model of coping with psychological stress."}
{"id": "PMID:469107", "title": "Comparing psychological and pharmacological treatments for hyperkinetic boys and their classmates.", "content": "This study compares the short-term effects of methylphenidate and of teacher consultation on the on-task behavior of diagnosed hyperkinetic outpatient boys and selected classmates. Statistically significant treatment effects were found for both drug-treated and behaviorally treated hyperkinetic boys; the size of these effects did not differ between the two types of treatment. Within the behavioral group, the treatment effect spilled over, so that there was also a significant treatment effect on overactive classmates of the behaviorally treated hyperkinetic children and a trend toward a significant treatment effect on their average classmates. Some implications of the findings are discussed.", "contents": "Comparing psychological and pharmacological treatments for hyperkinetic boys and their classmates. This study compares the short-term effects of methylphenidate and of teacher consultation on the on-task behavior of diagnosed hyperkinetic outpatient boys and selected classmates. Statistically significant treatment effects were found for both drug-treated and behaviorally treated hyperkinetic boys; the size of these effects did not differ between the two types of treatment. Within the behavioral group, the treatment effect spilled over, so that there was also a significant treatment effect on overactive classmates of the behaviorally treated hyperkinetic children and a trend toward a significant treatment effect on their average classmates. Some implications of the findings are discussed."}
{"id": "PMID:469108", "title": "The acute effects of caffeine on evoked response, vigilance, and activity level in hyperkinetic children.", "content": "Seventeen hyperkinetic children who had previously responded to sympathomimetic amines were given three different dosages of caffeine in counterbalanced order (placebo, and low and high doses equivalent to one and three cups of coffee). One hour following ingestions they were tested, double-blind on measures of visual evoked response, alpha time, vigilance, and activity level. There was a significant effect on evoked response. The behavioral measures tended to be affected in a dose-related manner but not to a statistically significant degree. It is concluded that although centrally active, caffeine does not show the congruence between behavioral and central effects that other stimulants useful in behavioral management have shown.", "contents": "The acute effects of caffeine on evoked response, vigilance, and activity level in hyperkinetic children. Seventeen hyperkinetic children who had previously responded to sympathomimetic amines were given three different dosages of caffeine in counterbalanced order (placebo, and low and high doses equivalent to one and three cups of coffee). One hour following ingestions they were tested, double-blind on measures of visual evoked response, alpha time, vigilance, and activity level. There was a significant effect on evoked response. The behavioral measures tended to be affected in a dose-related manner but not to a statistically significant degree. It is concluded that although centrally active, caffeine does not show the congruence between behavioral and central effects that other stimulants useful in behavioral management have shown."}
{"id": "PMID:469109", "title": "Cognitive, social, and other correlates of specific reading retardation.", "content": "Children with specific reading retardation were matched with controls on the basis of age, sex, intelligence, and (less successfully) parental education. All subjects were tested on a variety of automated performance tests including the Matching Familiar Figures (MFF) Task, Auditory-Visual Integration, Short-Term Memory, the Continuous Performance Task (CPT), and Motor Performance. All children were assessed socially using the Conners Teacher Questionnaire, and reading-retarded children were rated on the Behavior Problem Checklist. Finally, all children were screened with the Finger Twitch Test and were assessed for left-right awareness. Statistical analysis suggested that the following performance tests discriminated between the groups in this respective order of importance: MFF (Accuracy), Auditory-Visual Integration, and the CPT. The Short-Term Memory Task, MFF Decision Time, and Motor Performance did not distinguish between the groups. Multiple regression equations suggested that scores on the performance tests could account for about 40% of the variance in reading attainment. Several factors from the rating scales (notably those pertaining to attention and anxiety) and left-right awareness also discriminated between the groups.", "contents": "Cognitive, social, and other correlates of specific reading retardation. Children with specific reading retardation were matched with controls on the basis of age, sex, intelligence, and (less successfully) parental education. All subjects were tested on a variety of automated performance tests including the Matching Familiar Figures (MFF) Task, Auditory-Visual Integration, Short-Term Memory, the Continuous Performance Task (CPT), and Motor Performance. All children were assessed socially using the Conners Teacher Questionnaire, and reading-retarded children were rated on the Behavior Problem Checklist. Finally, all children were screened with the Finger Twitch Test and were assessed for left-right awareness. Statistical analysis suggested that the following performance tests discriminated between the groups in this respective order of importance: MFF (Accuracy), Auditory-Visual Integration, and the CPT. The Short-Term Memory Task, MFF Decision Time, and Motor Performance did not distinguish between the groups. Multiple regression equations suggested that scores on the performance tests could account for about 40% of the variance in reading attainment. Several factors from the rating scales (notably those pertaining to attention and anxiety) and left-right awareness also discriminated between the groups."}
{"id": "PMID:469110", "title": "Types of private speech produced by hyperactive and nonhyperactive boys.", "content": "Types and amount of private speech (audible talking that is not addressed to another person) were assessed during the free play of 16 hyperactive and 16 nonhyperactive boys. Verbalizations were coded into nine categories that denoted the boys' level of use of verbal control of their own behavior (Luria, 1961; Kohlberg, Yeager, & Hjertholm; 1968). Differences in amount and type of private speech between hyperactive and nonhyperactive boys were found to indicate that hyperactive boys may be presenting a specific or general cognitive lag in development. Treatment ramifications are discussed.", "contents": "Types of private speech produced by hyperactive and nonhyperactive boys. Types and amount of private speech (audible talking that is not addressed to another person) were assessed during the free play of 16 hyperactive and 16 nonhyperactive boys. Verbalizations were coded into nine categories that denoted the boys' level of use of verbal control of their own behavior (Luria, 1961; Kohlberg, Yeager, & Hjertholm; 1968). Differences in amount and type of private speech between hyperactive and nonhyperactive boys were found to indicate that hyperactive boys may be presenting a specific or general cognitive lag in development. Treatment ramifications are discussed."}
{"id": "PMID:469111", "title": "Rating scales for hyperactivity: concurrent validity, reliability, and decisions to label for the Conners and Davids Abbreviated Scales.", "content": "Rating scales have become the instrument of choice in labeling and assessing change in behavior of hyperactive children. However, several criticisms have recently have levied against their use. The present investigation examined the concurrent validity, and inter- and intrarater reliability for the Abbreviated Teacer Questionnaire (ATQ, Conners, 1973) and the Rating Scales for Hyperkinesis (Davids, 1971). Sixteen teachers from two special and two regular schools (grades 1-4) rated 211 normal and 49 special children using both scales. High correlations were found suggesting excellent predictability between scales and considerable stability across time and rater. Lower scores on a subsequent rating relative to an initial rating were demonstrated, dependent on time between ratings but independent of (a) teacher expectation of treatment gains, (b) bias produced by rating selected children, and (c) whether children were hyperactive or normal. Use of initial and infrequent rating scores versus subsequent, closely spaced ratings was related to the rater's objective (e.g., diagnosis, treatment, or assessment).", "contents": "Rating scales for hyperactivity: concurrent validity, reliability, and decisions to label for the Conners and Davids Abbreviated Scales. Rating scales have become the instrument of choice in labeling and assessing change in behavior of hyperactive children. However, several criticisms have recently have levied against their use. The present investigation examined the concurrent validity, and inter- and intrarater reliability for the Abbreviated Teacer Questionnaire (ATQ, Conners, 1973) and the Rating Scales for Hyperkinesis (Davids, 1971). Sixteen teachers from two special and two regular schools (grades 1-4) rated 211 normal and 49 special children using both scales. High correlations were found suggesting excellent predictability between scales and considerable stability across time and rater. Lower scores on a subsequent rating relative to an initial rating were demonstrated, dependent on time between ratings but independent of (a) teacher expectation of treatment gains, (b) bias produced by rating selected children, and (c) whether children were hyperactive or normal. Use of initial and infrequent rating scores versus subsequent, closely spaced ratings was related to the rater's objective (e.g., diagnosis, treatment, or assessment)."}
{"id": "PMID:469112", "title": "Personality patterns of adolescent poor and good sleepers.", "content": "To determine whether poor sleepers have a unique personality constellation significantly different from that of good sleepers, a sample of 162 emotionally disturbed adolescent poor sleepers was compared to a sample of 153 emotionally disturbed adolescent good sleepers on standardized personality instruments. Poor sleepers were found to have a high incidence of neurotic psychopathology with personality patterns characterized by depression, fearfulness, inhibition, anxiety, and rumination. In contrast, good sleepers showed quasi-healthy and/or characterological patterns. While this study does not resolve cause and effect relationships, it does establish a highly significant relationship between neuroticism and sleep disturbance for adolescent boys and girls, and also demonstrates a similarity of personality dynamics and patterns between adult and adolescent samples of patients with sleep disturbances.", "contents": "Personality patterns of adolescent poor and good sleepers. To determine whether poor sleepers have a unique personality constellation significantly different from that of good sleepers, a sample of 162 emotionally disturbed adolescent poor sleepers was compared to a sample of 153 emotionally disturbed adolescent good sleepers on standardized personality instruments. Poor sleepers were found to have a high incidence of neurotic psychopathology with personality patterns characterized by depression, fearfulness, inhibition, anxiety, and rumination. In contrast, good sleepers showed quasi-healthy and/or characterological patterns. While this study does not resolve cause and effect relationships, it does establish a highly significant relationship between neuroticism and sleep disturbance for adolescent boys and girls, and also demonstrates a similarity of personality dynamics and patterns between adult and adolescent samples of patients with sleep disturbances."}
{"id": "PMID:469113", "title": "Developmental recall lag in learning-disabled children: perceptual deficit or verbal mediation deficiency?", "content": "To investigate the development of mediational deficiencies in verbal and nonverbal visual short-term memory of learning-disabled children, the recall task of Atkinson, Hansen, and Bernback was administered to learning-disabled children in two experimental conditions. In Experiment 1 no significant differences on nonverbal short-term memory recall between normal and learning-disabled children were found. Similar recall responses (e.g., middle response bias, primacy effects, and recency effects) were found for both groups. Nonverbal recall was comparable for disabled and normal children as suggested by stimulus content and association scores. Experiment 2 found that while the effects of overt rehearsal on pretrained labels on learning-disabled children's recall was negligible, labels provided superior recall for normal children. Results suggested that learning-disabled children suffer from a verbal mediational deficiency consistent with Flavell's (1970) mediation deficiency hypothesis.", "contents": "Developmental recall lag in learning-disabled children: perceptual deficit or verbal mediation deficiency? To investigate the development of mediational deficiencies in verbal and nonverbal visual short-term memory of learning-disabled children, the recall task of Atkinson, Hansen, and Bernback was administered to learning-disabled children in two experimental conditions. In Experiment 1 no significant differences on nonverbal short-term memory recall between normal and learning-disabled children were found. Similar recall responses (e.g., middle response bias, primacy effects, and recency effects) were found for both groups. Nonverbal recall was comparable for disabled and normal children as suggested by stimulus content and association scores. Experiment 2 found that while the effects of overt rehearsal on pretrained labels on learning-disabled children's recall was negligible, labels provided superior recall for normal children. Results suggested that learning-disabled children suffer from a verbal mediational deficiency consistent with Flavell's (1970) mediation deficiency hypothesis."}
{"id": "PMID:469114", "title": "Behavior change in children at a therapeutic summer camp as a function of feedback and individual versus group contingencies.", "content": "Two contigency systems, individual and group, were evaluated for their effects on children's behavior during small group meetings. Forty-eight children, ages 8 through 14, attended a 7-week residential camp program operated on a token economy system. Ratings of the campers' behavior were made by the counselors on a 5-point rating scale at the daily meetings. Each camper received both contingency programs, in counterbalanced order. Data were gathered during baseline, two feedback and two treatment phases. An analysis of variance for repeated measures was performed on the mean weekly camper scores. The two systems were equally effective in significantly increasing the amount of desirable behavior, and there was no effect on behavior due to feedback alone. The results are discussed in terms of advantages and disadvantages of each type of contingency system, with some suggestions made regarding areas of further investigation.", "contents": "Behavior change in children at a therapeutic summer camp as a function of feedback and individual versus group contingencies. Two contigency systems, individual and group, were evaluated for their effects on children's behavior during small group meetings. Forty-eight children, ages 8 through 14, attended a 7-week residential camp program operated on a token economy system. Ratings of the campers' behavior were made by the counselors on a 5-point rating scale at the daily meetings. Each camper received both contingency programs, in counterbalanced order. Data were gathered during baseline, two feedback and two treatment phases. An analysis of variance for repeated measures was performed on the mean weekly camper scores. The two systems were equally effective in significantly increasing the amount of desirable behavior, and there was no effect on behavior due to feedback alone. The results are discussed in terms of advantages and disadvantages of each type of contingency system, with some suggestions made regarding areas of further investigation."}
{"id": "PMID:469115", "title": "Dimensions of problem behavior in deaf children.", "content": "The Behavior Problem Checklist was completed by the teachers of 192 deaf students who attended a special day school for the deaf. Three separate factor analyses were performed. Four factors that correspond to the dimensions found in earlier research and previously labeled conduct disorder, personality problems, immaturity-inadequacy, and socialized delinquency were found. An additional factor labeled passive inferiority was also extracted.", "contents": "Dimensions of problem behavior in deaf children. The Behavior Problem Checklist was completed by the teachers of 192 deaf students who attended a special day school for the deaf. Three separate factor analyses were performed. Four factors that correspond to the dimensions found in earlier research and previously labeled conduct disorder, personality problems, immaturity-inadequacy, and socialized delinquency were found. An additional factor labeled passive inferiority was also extracted."}
{"id": "PMID:469117", "title": "Measurement of the potency of pollen extract by radioimmunoprecipitation.", "content": "We describe a novel procedure for standardization of allergen extracts using radioiodinated crude extracts. Radiolabeled extracts were reacted with immunoglogulin E (IgE) antibody, and the resulting complexes precipitated by anti-IgE. Experiments with ragweed pollen, grass pollen, honeybee venom, and Alternaria extracts revealed that 1% to 14% of the radioactivity was precipitated, these percentages ranged from 2 to 20 times blank values. Binding of labeled ragweed extracts to IgE antibody could be inhibited by unlabeled extracts and linear inhibition responses could be derived. The potencies of 11 short ragweed extracts were ranked by their inhibitory capacities in such an IgE radioimmunoprecipitation (RIP) test and compared to potencies determined by radioallergosorbent test (RAST) inhibition; these were related, rs = +0.97 (p less than 0.001). Labeled extracts were also reacted with IgG antibodies, and the resulting complexes were precipitated by anti-IgG. From 1% to 34% of the radioactivity associated with the various extracts was precipitated by anti-IgG, and these percentages were from 2 to 20 times blank values. The reaction of IgG antibody with radiolabeled pollen extracts could be inhibited by unlabeled extract, and in the case of short ragweed the measurements of potencies using IgG and IgE antibodies were correlated, rs = +0.96 (p less than 0.001), suggesting that similar determinants were recognized by both IgG and IgE classes. The RIP procedure provides an alternative to the RAST for the standardization of allergy extracts and eliminates the need for affinity chromatography-purified antibody to IgE.", "contents": "Measurement of the potency of pollen extract by radioimmunoprecipitation. We describe a novel procedure for standardization of allergen extracts using radioiodinated crude extracts. Radiolabeled extracts were reacted with immunoglogulin E (IgE) antibody, and the resulting complexes precipitated by anti-IgE. Experiments with ragweed pollen, grass pollen, honeybee venom, and Alternaria extracts revealed that 1% to 14% of the radioactivity was precipitated, these percentages ranged from 2 to 20 times blank values. Binding of labeled ragweed extracts to IgE antibody could be inhibited by unlabeled extracts and linear inhibition responses could be derived. The potencies of 11 short ragweed extracts were ranked by their inhibitory capacities in such an IgE radioimmunoprecipitation (RIP) test and compared to potencies determined by radioallergosorbent test (RAST) inhibition; these were related, rs = +0.97 (p less than 0.001). Labeled extracts were also reacted with IgG antibodies, and the resulting complexes were precipitated by anti-IgG. From 1% to 34% of the radioactivity associated with the various extracts was precipitated by anti-IgG, and these percentages were from 2 to 20 times blank values. The reaction of IgG antibody with radiolabeled pollen extracts could be inhibited by unlabeled extract, and in the case of short ragweed the measurements of potencies using IgG and IgE antibodies were correlated, rs = +0.96 (p less than 0.001), suggesting that similar determinants were recognized by both IgG and IgE classes. The RIP procedure provides an alternative to the RAST for the standardization of allergy extracts and eliminates the need for affinity chromatography-purified antibody to IgE."}
{"id": "PMID:469119", "title": "Nasal challenge testing in grass pollen hay fever.", "content": "Nasal sensitivity to rye grass pollen allergens was evaluated by provocation testing in patients with hay fever due to grass pollen using measurements of nasal airways resistance (NAR), a reproducible system for delivery of allergen, and stringent criteria for allergen storage. Reproducibility was assessed in 24 subjects with hay fever by nasal provocation with serial dilutions of Lolium perenne allergens on 3 occasions: during the grass pollen season, immediately after the season, and in early winter. Threshold doses of allergen required to double the saline control NAR or to provoke persistent sneezing and rhinorrhea were slightly higher 1 mo after the pollen season, but there was no significant differences between threshold doses during the pollen season and 8 mo later. When the threshold doses during challenges were exceeded, there were late reactions in 4 of 24 patients. Normal subjects and patients with perennial rhinitis and with negative skin tests to L. perenne extract were unresponsive in nasal challenge tests.", "contents": "Nasal challenge testing in grass pollen hay fever. Nasal sensitivity to rye grass pollen allergens was evaluated by provocation testing in patients with hay fever due to grass pollen using measurements of nasal airways resistance (NAR), a reproducible system for delivery of allergen, and stringent criteria for allergen storage. Reproducibility was assessed in 24 subjects with hay fever by nasal provocation with serial dilutions of Lolium perenne allergens on 3 occasions: during the grass pollen season, immediately after the season, and in early winter. Threshold doses of allergen required to double the saline control NAR or to provoke persistent sneezing and rhinorrhea were slightly higher 1 mo after the pollen season, but there was no significant differences between threshold doses during the pollen season and 8 mo later. When the threshold doses during challenges were exceeded, there were late reactions in 4 of 24 patients. Normal subjects and patients with perennial rhinitis and with negative skin tests to L. perenne extract were unresponsive in nasal challenge tests."}
{"id": "PMID:469133", "title": "Streamlining and implementing nutritional assessment: the dietary approach.", "content": "Early methods of assessing food intake were developed to provide nutrition services for individuals in health care. Dietary methodologies increased in number and complexity as they were used in nutrition surveys of populations and as tools for nutrition research. Recent expansion of community health programs with nutrition components, and greater emphasis on nutritional assessment in health care require the use of streamlined methods of dietary inquiry and screening. The specialized knowledge and skills of the nutritionist/dietitian are best reserved for persons identified through screening as being at high risk for malnutrition.", "contents": "Streamlining and implementing nutritional assessment: the dietary approach. Early methods of assessing food intake were developed to provide nutrition services for individuals in health care. Dietary methodologies increased in number and complexity as they were used in nutrition surveys of populations and as tools for nutrition research. Recent expansion of community health programs with nutrition components, and greater emphasis on nutritional assessment in health care require the use of streamlined methods of dietary inquiry and screening. The specialized knowledge and skills of the nutritionist/dietitian are best reserved for persons identified through screening as being at high risk for malnutrition."}
{"id": "PMID:469134", "title": "Nutrient analysis of Exchange Lists for Meal Planning. I. Variation in nutrient levels.", "content": "Using available food composition data, individual Exchange Lists within the revised Exchange Lists for Meal Planning were evaluated for calories; protein; fat; calcium; phosphorus; iron; sodium; potassium; vitamin A; thiamin; riboflavin; niacin; ascorbic acid; saturated, oleic, and linoleic fatty acids; cholesterol; and crude fiber. Mean values, standard deviations, and ranges are reported for the above nutrients. Mean values agreed well with those suggested in the booklet for grams of carbohydrates, protein, and fat and for calories for all lists except milk. However, a wide range in values exists for food within each Exchange List.", "contents": "Nutrient analysis of Exchange Lists for Meal Planning. I. Variation in nutrient levels. Using available food composition data, individual Exchange Lists within the revised Exchange Lists for Meal Planning were evaluated for calories; protein; fat; calcium; phosphorus; iron; sodium; potassium; vitamin A; thiamin; riboflavin; niacin; ascorbic acid; saturated, oleic, and linoleic fatty acids; cholesterol; and crude fiber. Mean values, standard deviations, and ranges are reported for the above nutrients. Mean values agreed well with those suggested in the booklet for grams of carbohydrates, protein, and fat and for calories for all lists except milk. However, a wide range in values exists for food within each Exchange List."}
{"id": "PMID:469135", "title": "Nutrient analysis of Exchange Lists for Meal Planning. II. Nutrient density food profiles.", "content": "Nutrietn density profiles, generated for the individual subgroups within the revised Exchange Lists for Meal Planning, are presented. The authors suggest that the profiles would be useful for educational purposes.", "contents": "Nutrient analysis of Exchange Lists for Meal Planning. II. Nutrient density food profiles. Nutrietn density profiles, generated for the individual subgroups within the revised Exchange Lists for Meal Planning, are presented. The authors suggest that the profiles would be useful for educational purposes."}
{"id": "PMID:469136", "title": "Weight loss over time. Concomitants and consequences of a decreasing rate.", "content": "A decelerating rate of weight loss occurred among women participating in a community-based weight-control program. The rate of change may be due to greater food intake or to change in the level of fluid loss, in metabolic rate, and/or in the amount of physical activity. As these changes may affect motivation to persevere, they should be explained at the start of treatment, and program participants should be encouraged to maintain a gradually increasing level of physical activity. Disillusionment with the failure to sustain the initial rapid weight loss may be a factor that better predicts attrition than the demographic, personal history, or psychologic factors that have previously been studies.", "contents": "Weight loss over time. Concomitants and consequences of a decreasing rate. A decelerating rate of weight loss occurred among women participating in a community-based weight-control program. The rate of change may be due to greater food intake or to change in the level of fluid loss, in metabolic rate, and/or in the amount of physical activity. As these changes may affect motivation to persevere, they should be explained at the start of treatment, and program participants should be encouraged to maintain a gradually increasing level of physical activity. Disillusionment with the failure to sustain the initial rapid weight loss may be a factor that better predicts attrition than the demographic, personal history, or psychologic factors that have previously been studies."}
{"id": "PMID:469137", "title": "Protein quality of several multi-component, commercially prepared foods.", "content": "Eleven commercial food products that may be considered by consumers to be major sources of protein were evaluated. Most of these items contained significant amounts of carbohydrate in the form of cereal products or purified sugars. Seven of the products were at least equal to casein in protein efficiency ratio (PER). Canned spaghetti and meat balls, the TV dinner, canned chicken-and-vegetable baby food, and food bars had PER values between 91 and 73 per cent of that of casein, while PER values for ground beef and high-protein cereal were significantly higher than casein.", "contents": "Protein quality of several multi-component, commercially prepared foods. Eleven commercial food products that may be considered by consumers to be major sources of protein were evaluated. Most of these items contained significant amounts of carbohydrate in the form of cereal products or purified sugars. Seven of the products were at least equal to casein in protein efficiency ratio (PER). Canned spaghetti and meat balls, the TV dinner, canned chicken-and-vegetable baby food, and food bars had PER values between 91 and 73 per cent of that of casein, while PER values for ground beef and high-protein cereal were significantly higher than casein."}
{"id": "PMID:469138", "title": "Dietary management of patients with diabetes treated by hemodialysis.", "content": "Early experience with the treatment of patients with insulin-dependent diabetes and renal failure by chronic hemodialysis indicated a high mortality and increased incidence of medical complications. Since 1972, a marked improvement in survival and reduction in incidence of complications has been attributed to more rigorous control of fluid overload, hypertension, and blood sugar levels by insulin therapy and careful dietary management. A diet has been developed which combines the diet used by dialysis patients with suitable modifications for the insulin-dependent patient with diabetes. The importance of patient education is stressed in an attempt to improve patient compliance.", "contents": "Dietary management of patients with diabetes treated by hemodialysis. Early experience with the treatment of patients with insulin-dependent diabetes and renal failure by chronic hemodialysis indicated a high mortality and increased incidence of medical complications. Since 1972, a marked improvement in survival and reduction in incidence of complications has been attributed to more rigorous control of fluid overload, hypertension, and blood sugar levels by insulin therapy and careful dietary management. A diet has been developed which combines the diet used by dialysis patients with suitable modifications for the insulin-dependent patient with diabetes. The importance of patient education is stressed in an attempt to improve patient compliance."}
{"id": "PMID:469143", "title": "Comparison of mortality rates for various surgical operations according to age groups, 1951-1977.", "content": "A comparison was made of the mortality rates for various surgical operations, by age groups and by time periods. The age groups were: under 60, 60-69, 70-79, and 80 or over. The three time periods were: 1951-1955, 1956-1967, and 1967-1977. Only slight improvement was noted in these mortality rates despite the fact that during the 1951-1977 period many improvements were made in anesthesia, surgical techniques, antibiotic therapy, intensive-care units, and presumably the better understanding of disease. Further improvements will depend almost exclusively upon studying the basic problems of old age, chiefly atherosclerosis with its effects on the heart, blood vessels, brain, respiratory system and urinary tract. Unless this is done, there is no prospect of great improvement in operative results, and no basis for believing that the operative risk in the aged is the same as in the young. Unless the physiologic reserves of the aged can be improved, the surgical mortality rates likely will remain static.", "contents": "Comparison of mortality rates for various surgical operations according to age groups, 1951-1977. A comparison was made of the mortality rates for various surgical operations, by age groups and by time periods. The age groups were: under 60, 60-69, 70-79, and 80 or over. The three time periods were: 1951-1955, 1956-1967, and 1967-1977. Only slight improvement was noted in these mortality rates despite the fact that during the 1951-1977 period many improvements were made in anesthesia, surgical techniques, antibiotic therapy, intensive-care units, and presumably the better understanding of disease. Further improvements will depend almost exclusively upon studying the basic problems of old age, chiefly atherosclerosis with its effects on the heart, blood vessels, brain, respiratory system and urinary tract. Unless this is done, there is no prospect of great improvement in operative results, and no basis for believing that the operative risk in the aged is the same as in the young. Unless the physiologic reserves of the aged can be improved, the surgical mortality rates likely will remain static."}
{"id": "PMID:469146", "title": "Fluphenazine enanthate in the outpatient treatment of late paraphrenia.", "content": "Fluphenazine enanthate parenterally was compared to an antipsychotic drug (haloperidol) given orally for the \"crisis intervention\" treatment of elderly outpatients with late paraphrenia. In the fluphenazine group, 11 of 13 patients improved, compared to only 3 of 13 in the oral drug group (p less than 0.01). The superiority of fluphenazine probably was attributable to improved compliance with the prescribed regimen by patients receiving parenteral depot medication. Despite the advanced age of the subjects, significant adverse drug effects were uncommon.", "contents": "Fluphenazine enanthate in the outpatient treatment of late paraphrenia. Fluphenazine enanthate parenterally was compared to an antipsychotic drug (haloperidol) given orally for the \"crisis intervention\" treatment of elderly outpatients with late paraphrenia. In the fluphenazine group, 11 of 13 patients improved, compared to only 3 of 13 in the oral drug group (p less than 0.01). The superiority of fluphenazine probably was attributable to improved compliance with the prescribed regimen by patients receiving parenteral depot medication. Despite the advanced age of the subjects, significant adverse drug effects were uncommon."}
{"id": "PMID:469147", "title": "Rehabilitation of a patient with lower-extremity quadriprostheses.", "content": "A case report is presented that demonstrates the successful rehabilitation of an 84-year-old woman who had undergone successive surgical procedures resulting in bilateral total hip replacement and bilateral prostheses for below-knee amputations, i.e., quadriprostheses. It is emphasized that age per se should not be a deterrent to energetic rehabilitation efforts.", "contents": "Rehabilitation of a patient with lower-extremity quadriprostheses. A case report is presented that demonstrates the successful rehabilitation of an 84-year-old woman who had undergone successive surgical procedures resulting in bilateral total hip replacement and bilateral prostheses for below-knee amputations, i.e., quadriprostheses. It is emphasized that age per se should not be a deterrent to energetic rehabilitation efforts."}
{"id": "PMID:469148", "title": "Identification of normal aging and disease-related processes by health care professionals.", "content": "A 40-item quiz on aging was given to 46 health care professionals, before they participated in a workshop dealing with the care of the elderly patient. In the quiz, they were asked to identify physiologic and functional conditions either as normal aging processes or disease-related processes. In general the results indicated that disease processes were the easier entities to identify, and that the workshop experience enhanced this effect. Ability to identify functional disorders correctly did not differ significantly from the ability to identify physiologic symptoms correctly. Implications for the general health and the mental health of older adults are discussed.", "contents": "Identification of normal aging and disease-related processes by health care professionals. A 40-item quiz on aging was given to 46 health care professionals, before they participated in a workshop dealing with the care of the elderly patient. In the quiz, they were asked to identify physiologic and functional conditions either as normal aging processes or disease-related processes. In general the results indicated that disease processes were the easier entities to identify, and that the workshop experience enhanced this effect. Ability to identify functional disorders correctly did not differ significantly from the ability to identify physiologic symptoms correctly. Implications for the general health and the mental health of older adults are discussed."}
{"id": "PMID:469149", "title": "Central-nervous-system stimulants: appraisal of use in geropsychiatric patients.", "content": "CNS stimulants such as methylphenidate and pentylenetetrazol are currently prescribed for the treatment of aged persons with symptoms of senility. A review of clinical studies conducted with these compounds indicates that firm evidence of efficacy is lacking. Because of the risks associated with high doses of such stimulants in the aged, it would seem that these compounds are of limited utility in geriatric psychiatry.", "contents": "Central-nervous-system stimulants: appraisal of use in geropsychiatric patients. CNS stimulants such as methylphenidate and pentylenetetrazol are currently prescribed for the treatment of aged persons with symptoms of senility. A review of clinical studies conducted with these compounds indicates that firm evidence of efficacy is lacking. Because of the risks associated with high doses of such stimulants in the aged, it would seem that these compounds are of limited utility in geriatric psychiatry."}
{"id": "PMID:469151", "title": "The vaginal flora after natural or surgical menopause.", "content": "A study was made of the cervical and upper vaginal flora in menopausal women, in an attempt to determine whether such women are predisposed to infections when undergoing gynecologic operations. The series comprised 72 women (age range, 44-80 years) classified as follows: a) 35 with a natural menopause, b) 18 with a surgical menopause induced by abdominal panhysterectomy, and c) 19 postmenopausal women after vaginal hysterectomy performed for genital prolapse. The vaginal flora of these menopausal women consisted predominantly of Gram-positive bacteria which, together with the Gram-negative bacteria, conformed to the normal pattern of microorganisms in the vagina. Such bacteria also are found in women of child-bearing age, but occasionally they can be pathogenic. Despite the inherent biohormonal changes of the memopause, expressed especially by the low estrogen level, 26.4 percent of the cultures in our study were sterile, even after major vaginal operations. Thus, the pattern of the vaginal flora, even though occasionally pathogen, should not be a contraindication to gynecologic surgical procedures in postmenopausal women. Even when vaginal cultures show the presence of these bacteria, it does not seem necessary to use prophylactic antibiotic and hormonal therapy routinely. Rather, it should be given selectively, depending upon the local state of the tissues and upon the postoperative course.", "contents": "The vaginal flora after natural or surgical menopause. A study was made of the cervical and upper vaginal flora in menopausal women, in an attempt to determine whether such women are predisposed to infections when undergoing gynecologic operations. The series comprised 72 women (age range, 44-80 years) classified as follows: a) 35 with a natural menopause, b) 18 with a surgical menopause induced by abdominal panhysterectomy, and c) 19 postmenopausal women after vaginal hysterectomy performed for genital prolapse. The vaginal flora of these menopausal women consisted predominantly of Gram-positive bacteria which, together with the Gram-negative bacteria, conformed to the normal pattern of microorganisms in the vagina. Such bacteria also are found in women of child-bearing age, but occasionally they can be pathogenic. Despite the inherent biohormonal changes of the memopause, expressed especially by the low estrogen level, 26.4 percent of the cultures in our study were sterile, even after major vaginal operations. Thus, the pattern of the vaginal flora, even though occasionally pathogen, should not be a contraindication to gynecologic surgical procedures in postmenopausal women. Even when vaginal cultures show the presence of these bacteria, it does not seem necessary to use prophylactic antibiotic and hormonal therapy routinely. Rather, it should be given selectively, depending upon the local state of the tissues and upon the postoperative course."}
{"id": "PMID:469152", "title": "Age-related changes in endocrine and renal function in patients with essential hypertension.", "content": "Endocrine and renal functions were studied in 149 patients with essential hypertension by measuring plasma electrolytes, renin activity, creatinine and aldoserone, as well as the urinary excretion of creatinine and sodium chloride, before and during treatment for hypertension. Half of the patients responded to trichlormethiazide (thiazide-responsive group) but the other half did not (thiazide-unresponsive group). Systolic and diastolic blood pressures increased progressively uith age in the thiazide-unresponsive group, but were lower and did not progress with age in the thiazide-responsive group. There was no consistent difference in plasma renin activity between the thiazide-responsive and the thiazide-unresponsive groups. The fluctuation of plasma renin activity in response to an excess of sodium chloride or to thiazide treatment was reduced progressively with age. Creatinine clearance decreased and the blood urea nitrogen level increased with age. The age-related decrease of plasma renin activity is discussed on the light of the age-related impairment in the ability of the kidney to excrete sodium and water.", "contents": "Age-related changes in endocrine and renal function in patients with essential hypertension. Endocrine and renal functions were studied in 149 patients with essential hypertension by measuring plasma electrolytes, renin activity, creatinine and aldoserone, as well as the urinary excretion of creatinine and sodium chloride, before and during treatment for hypertension. Half of the patients responded to trichlormethiazide (thiazide-responsive group) but the other half did not (thiazide-unresponsive group). Systolic and diastolic blood pressures increased progressively uith age in the thiazide-unresponsive group, but were lower and did not progress with age in the thiazide-responsive group. There was no consistent difference in plasma renin activity between the thiazide-responsive and the thiazide-unresponsive groups. The fluctuation of plasma renin activity in response to an excess of sodium chloride or to thiazide treatment was reduced progressively with age. Creatinine clearance decreased and the blood urea nitrogen level increased with age. The age-related decrease of plasma renin activity is discussed on the light of the age-related impairment in the ability of the kidney to excrete sodium and water."}
{"id": "PMID:469153", "title": "\"Spontaneous\" atheroembolic disease as a cause of renal failure in the elderly.", "content": "An 86-year-old man with previous normal renal function was hospitalized because of renal insufficiency. He had a long history of atherosclerotic heart disease, mild hypertension and pulmonary embolism, requiring anticoagulant therapy. In view of the normal-sized kidneys and absence of casts in the urinary sediment, a diagnosis of atheroembolic renal disease was made. The patient's renal function deteriorated, but he refused hemodialysis. Death occurred within a few weeks. At autopsy, severe aortic atherosclerosis was observed and atheroembolic renal disease was confirmed as the cause of renal failure. Occasionally, renal failure can be the sole manifestation of spontaneous atheroembolic disease. This possibility should be considered if the physician is called upon to establish the diagnosis when renal insufficiency develops in atherosclerotic patients.", "contents": "\"Spontaneous\" atheroembolic disease as a cause of renal failure in the elderly. An 86-year-old man with previous normal renal function was hospitalized because of renal insufficiency. He had a long history of atherosclerotic heart disease, mild hypertension and pulmonary embolism, requiring anticoagulant therapy. In view of the normal-sized kidneys and absence of casts in the urinary sediment, a diagnosis of atheroembolic renal disease was made. The patient's renal function deteriorated, but he refused hemodialysis. Death occurred within a few weeks. At autopsy, severe aortic atherosclerosis was observed and atheroembolic renal disease was confirmed as the cause of renal failure. Occasionally, renal failure can be the sole manifestation of spontaneous atheroembolic disease. This possibility should be considered if the physician is called upon to establish the diagnosis when renal insufficiency develops in atherosclerotic patients."}
{"id": "PMID:469154", "title": "The Life Satisfaction Index-Form A as applied to older adults: technical note on scoring patterns.", "content": "The value of standardized scales for determining life satisfaction among older adults is being questioned, but use of the scales continues. The Life Satisfaction Index-Form A (LSIA) in its many variations continues to be among the most widely used scales for this purpose. In a comparison of two scoring methods (36-point vs. 18-point) for the LSIA, the lack of a significant difference indicated that a 36-point scale is not necessary. Caution in the use and interpretation of the variances between the two scoring methods is suggested.", "contents": "The Life Satisfaction Index-Form A as applied to older adults: technical note on scoring patterns. The value of standardized scales for determining life satisfaction among older adults is being questioned, but use of the scales continues. The Life Satisfaction Index-Form A (LSIA) in its many variations continues to be among the most widely used scales for this purpose. In a comparison of two scoring methods (36-point vs. 18-point) for the LSIA, the lack of a significant difference indicated that a 36-point scale is not necessary. Caution in the use and interpretation of the variances between the two scoring methods is suggested."}
{"id": "PMID:469155", "title": "Mitral valve prolapse in the elderly.", "content": "This study included 40 patients over 60 years of age with echocardiographic findings of mitral valve prolapse (MVP). Most of these patients were unaware of any cardiac disorder until the time of echocardiography. In the majority, the clinical manifestations were benign, and the duration of symptoms variable. Congestive heart failure (CHF) was noted in 10 patients (25 percent) who were unaware of having any cardiac disorders until the onset of their symptoms. In 5 patients (4 with CHF and 1 with endocarditis), surgical replacement of the prolapsed mitral valve was necessary. Endocarditis was present in 4 patients (10 percent), none of whom had been instructed in the prophylactic use of antibiotics. The physician's awareness of mitral valve prolapse in the elderly patient is important, since the disorder may not be as benign in aged patients as in younger ones, and life-threatening complications may occur.", "contents": "Mitral valve prolapse in the elderly. This study included 40 patients over 60 years of age with echocardiographic findings of mitral valve prolapse (MVP). Most of these patients were unaware of any cardiac disorder until the time of echocardiography. In the majority, the clinical manifestations were benign, and the duration of symptoms variable. Congestive heart failure (CHF) was noted in 10 patients (25 percent) who were unaware of having any cardiac disorders until the onset of their symptoms. In 5 patients (4 with CHF and 1 with endocarditis), surgical replacement of the prolapsed mitral valve was necessary. Endocarditis was present in 4 patients (10 percent), none of whom had been instructed in the prophylactic use of antibiotics. The physician's awareness of mitral valve prolapse in the elderly patient is important, since the disorder may not be as benign in aged patients as in younger ones, and life-threatening complications may occur."}
{"id": "PMID:469174", "title": "Behavior of dialifor, dimethoate, and methidathion in artificially fortified grape juice processed into wine.", "content": "Dialifor and methidathion were added to diluted \"Zinfandel\" grape concentrate at 25 ppm and dimethoate at 1.0 and 25 ppm prior to fermentation with Saccharomyces cerevisiae. The finished wine 56 days later contained 10% (2.5 ppm) of the dialifor, 46% (12 ppm) of the methidathion and 85% (21 and 0.98 ppm) of the dimethoate added to the grape must. Residues in wine stored at 24 degrees C dissipated by hydrolysis; half-lives in wine were 7 days for dialifor and methidathion and 30 days for dimethoate. Residues were unchanged in wine in frozen storage for one year. Analysis of seven commercial wines for dimethoate indicated less than 0.03 ppm dimethoate was present; identity could not be confirmed by thin-layer chromatography at this level.", "contents": "Behavior of dialifor, dimethoate, and methidathion in artificially fortified grape juice processed into wine. Dialifor and methidathion were added to diluted \"Zinfandel\" grape concentrate at 25 ppm and dimethoate at 1.0 and 25 ppm prior to fermentation with Saccharomyces cerevisiae. The finished wine 56 days later contained 10% (2.5 ppm) of the dialifor, 46% (12 ppm) of the methidathion and 85% (21 and 0.98 ppm) of the dimethoate added to the grape must. Residues in wine stored at 24 degrees C dissipated by hydrolysis; half-lives in wine were 7 days for dialifor and methidathion and 30 days for dimethoate. Residues were unchanged in wine in frozen storage for one year. Analysis of seven commercial wines for dimethoate indicated less than 0.03 ppm dimethoate was present; identity could not be confirmed by thin-layer chromatography at this level."}
{"id": "PMID:469175", "title": "Recovery studies of selenium using dry ashing procedure with ashing aid.", "content": "A previously reported dry ashing procedure for the decomposition of marine samples prior to arsenic determination is demonstrated to be equally applicable to the analysis of selenium. Quantitative recovery of selenium as inorganic or organic compounds in a variety of plants and animal tissues is demonstrated using 75Se tracer and X-ray fluorescence techniques. The dry ashing procedure is simpler than the official AOAC method, which uses a mixture of H2SO4, HNO3 and HC10(4).", "contents": "Recovery studies of selenium using dry ashing procedure with ashing aid. A previously reported dry ashing procedure for the decomposition of marine samples prior to arsenic determination is demonstrated to be equally applicable to the analysis of selenium. Quantitative recovery of selenium as inorganic or organic compounds in a variety of plants and animal tissues is demonstrated using 75Se tracer and X-ray fluorescence techniques. The dry ashing procedure is simpler than the official AOAC method, which uses a mixture of H2SO4, HNO3 and HC10(4)."}
{"id": "PMID:469176", "title": "Volatilization of 1,2-dibromo-3-chloropropane (DBCP) from soils.", "content": "The volatilization of DBCP from soils, as affected by the soil characteristics and application techniques, was studied in a laboratory experiment. The volatilization rate of DBCP applied in water was higher from sandy and silty loam soils than from clay soil. Water added after DBCP application acted as a soil cover, decreasing the volatilization rate. The results obtained with DBCP application in hexane to air-dry soils, indicate that adsorption could be an important factor in reducing the volatilization losses. Diffusion coefficients were calculated from the volatilization parameters, by using a simplified relationship between volatilization losses and diffusion through soil.", "contents": "Volatilization of 1,2-dibromo-3-chloropropane (DBCP) from soils. The volatilization of DBCP from soils, as affected by the soil characteristics and application techniques, was studied in a laboratory experiment. The volatilization rate of DBCP applied in water was higher from sandy and silty loam soils than from clay soil. Water added after DBCP application acted as a soil cover, decreasing the volatilization rate. The results obtained with DBCP application in hexane to air-dry soils, indicate that adsorption could be an important factor in reducing the volatilization losses. Diffusion coefficients were calculated from the volatilization parameters, by using a simplified relationship between volatilization losses and diffusion through soil."}
{"id": "PMID:469177", "title": "Effect of aldicarb on the growth, respiration and (14C) - glucose metabolism of Axotobacter chroococcum BEIJ.", "content": "Aldicarb [2-methyl-2(methyl thio) propionaldehyde-O-methyl carbamoyl oxime], which forms the active ingredient of a systemic oximic carbamate insecticide, at 2 ppm level did not affect the in vitro growth and respiration of Azotobacter chroococcum Beij, while concentrations at 5 ppm and 10 ppm levels were inhibitory. The insecticide treatment at 10 ppm level suppressed the assimilation of (14C) - glucose in the whole cells and in the cellular constitutents viz., cold - TCA soluble, hot TCA soluble fractions and insoluble residue. However, the 14C - incorporation in the alcohol soluble and alcohol-ether soluble fractions was enhanced indicating that aldicarb considerably altered the glucose metabolism of the organism.", "contents": "Effect of aldicarb on the growth, respiration and (14C) - glucose metabolism of Axotobacter chroococcum BEIJ. Aldicarb [2-methyl-2(methyl thio) propionaldehyde-O-methyl carbamoyl oxime], which forms the active ingredient of a systemic oximic carbamate insecticide, at 2 ppm level did not affect the in vitro growth and respiration of Azotobacter chroococcum Beij, while concentrations at 5 ppm and 10 ppm levels were inhibitory. The insecticide treatment at 10 ppm level suppressed the assimilation of (14C) - glucose in the whole cells and in the cellular constitutents viz., cold - TCA soluble, hot TCA soluble fractions and insoluble residue. However, the 14C - incorporation in the alcohol soluble and alcohol-ether soluble fractions was enhanced indicating that aldicarb considerably altered the glucose metabolism of the organism."}
{"id": "PMID:469178", "title": "A monitoring study of workers handling pesticides in warehouses and godowns.", "content": "Monitoring observations made on 60 operators involved in pesticide application work in godowns and warehouses and 60 matched control workers are reported. Occupational exposure history and medical history are noted. Biochemical investigations, plasma and RBC cholinesterase estimations are included along with medical examination of the workers. Workers were found to be mostly exposed to Celphos, DDVP, Malathion, Pyrethrum, etc, and the use of protective devices were very limited. Cases of significant reduction in plasma and RBC cholinesterase activity were found. Frequency of symptoms like dizziness, headache, lachrymation, burning sensation in eyes, nausea and anorexia, etc, were much more in the exposed workers. No cases of clinical poisoning attributable to occupational exposure to pesticides were reported by the workers.", "contents": "A monitoring study of workers handling pesticides in warehouses and godowns. Monitoring observations made on 60 operators involved in pesticide application work in godowns and warehouses and 60 matched control workers are reported. Occupational exposure history and medical history are noted. Biochemical investigations, plasma and RBC cholinesterase estimations are included along with medical examination of the workers. Workers were found to be mostly exposed to Celphos, DDVP, Malathion, Pyrethrum, etc, and the use of protective devices were very limited. Cases of significant reduction in plasma and RBC cholinesterase activity were found. Frequency of symptoms like dizziness, headache, lachrymation, burning sensation in eyes, nausea and anorexia, etc, were much more in the exposed workers. No cases of clinical poisoning attributable to occupational exposure to pesticides were reported by the workers."}
{"id": "PMID:469179", "title": "Metabolism of 2-chloro-N-isopropylacetanilide (propachlor) in the rat.", "content": "Eleven urinary metabolites from [14C]propachlor were either identified or characterized by mass spectrometry. Those identified were 2-[S-(N-acetyl)cysteinyl]-N-isopropylacetanilide, 2-(methylsulfonyl)-acetanilide, 4'-hydroxy-2-(methylsulfonyl)-acetanilide, and 4'-hydroxyacetanilide. Those characterized were N-(1-hydroxyisopropyl)-2-(methylsulfonyl) acetanilide and its glucuronide, the glucuronides of 4'-hydroxy-N-isopropyl-2-(methylsulfonyl)acetanilide, N-(1-hydroxyisopropyl) aniline, 4'-hydroxy-2-(methylsulfonyl)acetanilide, and either N-(1-hydroxy-isopropyl) acetanilide or 2-hydroxy-N-isopropylacetanilide.", "contents": "Metabolism of 2-chloro-N-isopropylacetanilide (propachlor) in the rat. Eleven urinary metabolites from [14C]propachlor were either identified or characterized by mass spectrometry. Those identified were 2-[S-(N-acetyl)cysteinyl]-N-isopropylacetanilide, 2-(methylsulfonyl)-acetanilide, 4'-hydroxy-2-(methylsulfonyl)-acetanilide, and 4'-hydroxyacetanilide. Those characterized were N-(1-hydroxyisopropyl)-2-(methylsulfonyl) acetanilide and its glucuronide, the glucuronides of 4'-hydroxy-N-isopropyl-2-(methylsulfonyl)acetanilide, N-(1-hydroxyisopropyl) aniline, 4'-hydroxy-2-(methylsulfonyl)acetanilide, and either N-(1-hydroxy-isopropyl) acetanilide or 2-hydroxy-N-isopropylacetanilide."}
{"id": "PMID:469180", "title": "Metabolic fate of dichloromethyl-O,O-diphenyl phosphonate (I) and dichloromethyl-O,O-Di-(p-nitrophenyl) phosphonate (II) in/on rice plants.", "content": "Metabolic fate of two dichloromethyl diaryl phosphonates (32P labelled) in/on rice plants was investigated. The test compounds were found to be less persistent on the surface of rice leaves with half lives 7.4 and 6.7 days respectively. Main degradation product from both the phosphonates were dichloromethyl phosphonic acid with trace of dichloromethyl-O-aryl phosphonate as a transitory intermediate product.", "contents": "Metabolic fate of dichloromethyl-O,O-diphenyl phosphonate (I) and dichloromethyl-O,O-Di-(p-nitrophenyl) phosphonate (II) in/on rice plants. Metabolic fate of two dichloromethyl diaryl phosphonates (32P labelled) in/on rice plants was investigated. The test compounds were found to be less persistent on the surface of rice leaves with half lives 7.4 and 6.7 days respectively. Main degradation product from both the phosphonates were dichloromethyl phosphonic acid with trace of dichloromethyl-O-aryl phosphonate as a transitory intermediate product."}
{"id": "PMID:469181", "title": "Prenatal carbon monoxide and adult evoked potentials in rats.", "content": "Hooded rats prenatally exposed to mild carbon monoxide hypoxia were studied as adults. Flash evoked potentials were recorded from the visual cortex of unanesthetized exposed and control rats at four different flash intensities. The amplitude of bothe the P1-N1 and N1-P2 components on the flash evoked potential were significantly greater in CO-exposed female rats than in control females. The experiments thus demonstrate long term alterations in CNS function from exposure to relatively mild levels of CO.", "contents": "Prenatal carbon monoxide and adult evoked potentials in rats. Hooded rats prenatally exposed to mild carbon monoxide hypoxia were studied as adults. Flash evoked potentials were recorded from the visual cortex of unanesthetized exposed and control rats at four different flash intensities. The amplitude of bothe the P1-N1 and N1-P2 components on the flash evoked potential were significantly greater in CO-exposed female rats than in control females. The experiments thus demonstrate long term alterations in CNS function from exposure to relatively mild levels of CO."}
{"id": "PMID:469182", "title": "Development of rat telencephalic neurons after prenatal X-irradiation.", "content": "Telencephalic neurons of rats, irradiated at day 15 of gestation with 125 R, develop synaptic connections on dendrites during maturation which appear to be normal spines in Golgi-stained light microscope preparations. At six weeks of postnatal age both control and irradiated rats have spiny dendritic processes on cortical pyramidal cells and caudate Golgi type II neurons. However, when the rats are 6 months old the irradiated rats have more neurons with beaded dendritic processes that lack spines or have only a few spines. These neurons resemble the neonatal neurons and are likely to be degenerating neurons. The apparently normal development of the neurons followed by degeneration in the irradiated rat has a parallel in previous reports of the delayed hyperactivity which develops in rats irradiated on the fifteenth gestational day.", "contents": "Development of rat telencephalic neurons after prenatal X-irradiation. Telencephalic neurons of rats, irradiated at day 15 of gestation with 125 R, develop synaptic connections on dendrites during maturation which appear to be normal spines in Golgi-stained light microscope preparations. At six weeks of postnatal age both control and irradiated rats have spiny dendritic processes on cortical pyramidal cells and caudate Golgi type II neurons. However, when the rats are 6 months old the irradiated rats have more neurons with beaded dendritic processes that lack spines or have only a few spines. These neurons resemble the neonatal neurons and are likely to be degenerating neurons. The apparently normal development of the neurons followed by degeneration in the irradiated rat has a parallel in previous reports of the delayed hyperactivity which develops in rats irradiated on the fifteenth gestational day."}
{"id": "PMID:469183", "title": "Tissue distribution of inhaled mercury vapor in acatalasemic mice.", "content": "Substantial differences were observed in the pattern of tissue deposition in normal versus acatalasemic mice after inhalation of radioactive mercury vapor. Liver deposition was approximately double and lung deposition approximately one-half the corresponding deposition in normal animals. These results lend further support to the idea that the hydrogen-peroxide catalase peroxidative pathway plays a role in the metabolism of inhaled mercury vapor.", "contents": "Tissue distribution of inhaled mercury vapor in acatalasemic mice. Substantial differences were observed in the pattern of tissue deposition in normal versus acatalasemic mice after inhalation of radioactive mercury vapor. Liver deposition was approximately double and lung deposition approximately one-half the corresponding deposition in normal animals. These results lend further support to the idea that the hydrogen-peroxide catalase peroxidative pathway plays a role in the metabolism of inhaled mercury vapor."}
{"id": "PMID:469184", "title": "Early onset and topographical distribution of hypertrophied astrocytes in hippocampus of aging rats: a quantitative study.", "content": "Our results indicate that the density of astrocytes exhibiting hypertrophy in the hippocampus increases dramatically and progressively with age, being significantly elevated even in mid-life (13 month) animals. However, the total population of astrocytic cells (hypertrophied and nonhypertrophied) in the quantified region is not significantly elevated. Further, the relative distributions of both hypertrophied and total glial cells remained remarkably constant at the three ages measured. Reactive hypertrophy of astrocytes therefore appears to occur \"in place\" and migration of astrocytes does not appear to be a major factor in the hippocampus during aging. Of particular note was the finding that astrocyte hypertrophy is far more prominent in synaptic terminal fields than in myelinated fibers of the same system.", "contents": "Early onset and topographical distribution of hypertrophied astrocytes in hippocampus of aging rats: a quantitative study. Our results indicate that the density of astrocytes exhibiting hypertrophy in the hippocampus increases dramatically and progressively with age, being significantly elevated even in mid-life (13 month) animals. However, the total population of astrocytic cells (hypertrophied and nonhypertrophied) in the quantified region is not significantly elevated. Further, the relative distributions of both hypertrophied and total glial cells remained remarkably constant at the three ages measured. Reactive hypertrophy of astrocytes therefore appears to occur \"in place\" and migration of astrocytes does not appear to be a major factor in the hippocampus during aging. Of particular note was the finding that astrocyte hypertrophy is far more prominent in synaptic terminal fields than in myelinated fibers of the same system."}
{"id": "PMID:469185", "title": "Nuclease digestion studies of mouse chromatin as a function of age.", "content": "Chromatin is organized into a repeating structure (nucleosome) made up of proteins and DNA. Micrococcal nuclease and DNAase I have been used to probe this structure in nuclear populations from three tissues (liver, brain and heart) of the inbred mouse strain C57BL at different ages. For those parameters examined in each tissue, chromatin contained essentially the same features of nucleosomal organization, regardless of the age of the mouse. Thus, the rate and extent of nuclease digestion, the size of the DNA repeat unit and nucleosome core are not significantly different as a function of age. However, the accessibility of internucleosomal DNA to micrococcal nuclease, as determined by measuring the DNA size distribution after nuclease cutting, may be partially limited in brain chromatin (but not liver or heart) of older animals. These results indicate that there are no gross, age-related changes in the conformational state or organization of chromatin in these tissues. The results do not exclude smaller alterations in chromatin which might occur with age and which the methodology employed might not be sensitive enough to detect.", "contents": "Nuclease digestion studies of mouse chromatin as a function of age. Chromatin is organized into a repeating structure (nucleosome) made up of proteins and DNA. Micrococcal nuclease and DNAase I have been used to probe this structure in nuclear populations from three tissues (liver, brain and heart) of the inbred mouse strain C57BL at different ages. For those parameters examined in each tissue, chromatin contained essentially the same features of nucleosomal organization, regardless of the age of the mouse. Thus, the rate and extent of nuclease digestion, the size of the DNA repeat unit and nucleosome core are not significantly different as a function of age. However, the accessibility of internucleosomal DNA to micrococcal nuclease, as determined by measuring the DNA size distribution after nuclease cutting, may be partially limited in brain chromatin (but not liver or heart) of older animals. These results indicate that there are no gross, age-related changes in the conformational state or organization of chromatin in these tissues. The results do not exclude smaller alterations in chromatin which might occur with age and which the methodology employed might not be sensitive enough to detect."}
{"id": "PMID:469186", "title": "Mental aging and its medico-psycho-social background in the very old Japanese.", "content": "By clinical observations of 115 centenarians and 742 nonagenarians, the actual state of mental aging of the very old Japanese and some factors relating to it are shown in this paper. A great difference in psychophysical functions was found between the centenarians and the nonagenarians. Most centenarians manifested a distinct decline in these functions, while a decline was less prominent in the 90-year-olds. There was a significant relationship between mental and physical functions. Those manifesting a prominent decline in intellectual ability showed more frequent reduction in physical ability as well, and vice versa. The degree of their intellectual ability was also related to the following factors: their sex, academic and occupational careers, some types of personality traits and personality changes. The mental decline was less prominent in males, in the educated and in those with a syntonic personality than in others.", "contents": "Mental aging and its medico-psycho-social background in the very old Japanese. By clinical observations of 115 centenarians and 742 nonagenarians, the actual state of mental aging of the very old Japanese and some factors relating to it are shown in this paper. A great difference in psychophysical functions was found between the centenarians and the nonagenarians. Most centenarians manifested a distinct decline in these functions, while a decline was less prominent in the 90-year-olds. There was a significant relationship between mental and physical functions. Those manifesting a prominent decline in intellectual ability showed more frequent reduction in physical ability as well, and vice versa. The degree of their intellectual ability was also related to the following factors: their sex, academic and occupational careers, some types of personality traits and personality changes. The mental decline was less prominent in males, in the educated and in those with a syntonic personality than in others."}
{"id": "PMID:469187", "title": "Environment and face-hand test performance in the institutionalized elderly.", "content": "Long term institutionalized elderly people were tested over nine months on the Face Hand (FH) test. Orinally other neuropsychological tests also were to be given, but most patients could only respond to FH at first. Experimental subjects were transferred either to an enriched stimulation environment or a token economy program while control subjects remained in custodial care settings. Analysis of variance interaction between experimental condition and time of testing indicated significant improvement in performance in the enriched environment compared to the token economy and control groups. In supplementary analyses on a subsample capable of other tests at the end of nine months, FH correlated significantly with some neuropsychological measures and not with social skills.", "contents": "Environment and face-hand test performance in the institutionalized elderly. Long term institutionalized elderly people were tested over nine months on the Face Hand (FH) test. Orinally other neuropsychological tests also were to be given, but most patients could only respond to FH at first. Experimental subjects were transferred either to an enriched stimulation environment or a token economy program while control subjects remained in custodial care settings. Analysis of variance interaction between experimental condition and time of testing indicated significant improvement in performance in the enriched environment compared to the token economy and control groups. In supplementary analyses on a subsample capable of other tests at the end of nine months, FH correlated significantly with some neuropsychological measures and not with social skills."}
{"id": "PMID:469188", "title": "Attitude change in older age: an experimental study.", "content": "The role of age in the attitude change process was experimentally studied by exposing older (Mean age: 77.5 years) and younger (Mean age: 19.7 years) women to persuasive information presented at different speeds. In general, the higher speed levels which were employed to decrease information reception resulted in less attitude change, but no overall age differences in the amount of attitude change were observed. In fact, the older women changed their attitudes somewhat more than the younger ones in the normal speed condition. Two mediating processes--positive evaluation and information reception--seemed to counteract each other in producing attitude change in the two age groups: the older women reacted more positively toward the presentation than the younger women, but they understood less of the presented information.", "contents": "Attitude change in older age: an experimental study. The role of age in the attitude change process was experimentally studied by exposing older (Mean age: 77.5 years) and younger (Mean age: 19.7 years) women to persuasive information presented at different speeds. In general, the higher speed levels which were employed to decrease information reception resulted in less attitude change, but no overall age differences in the amount of attitude change were observed. In fact, the older women changed their attitudes somewhat more than the younger ones in the normal speed condition. Two mediating processes--positive evaluation and information reception--seemed to counteract each other in producing attitude change in the two age groups: the older women reacted more positively toward the presentation than the younger women, but they understood less of the presented information."}
{"id": "PMID:469189", "title": "Age differences in the effects of perceptual noise.", "content": "Elderly (60 to 82 years) and young (18 to 25 years) subjects were tested on a visual task in which noise items were present but did not require processing to test the hypotheses that elderly persons have more difficulty ignoring irrelevent stimuli and suppressing response competition than do younger persons. Tachistoscopically presented displays contained a precued central target letter either alone or flanked by noise items that required (1) a response the same as, or (2) opposite to that required by the target letter, or (3) did not require a response in this task. No evidence was found to support differentially greater response slowing by the elderly due to the presence of irrelevant stimuli. Elderly persons showed significantly less effects of response competition, suggesting that the two groups processed the task information differently. The results suggest that age differences in effects of perceptual noise and response competition are not general phenomena, but depend upon the demand characteristics of the specific task.", "contents": "Age differences in the effects of perceptual noise. Elderly (60 to 82 years) and young (18 to 25 years) subjects were tested on a visual task in which noise items were present but did not require processing to test the hypotheses that elderly persons have more difficulty ignoring irrelevent stimuli and suppressing response competition than do younger persons. Tachistoscopically presented displays contained a precued central target letter either alone or flanked by noise items that required (1) a response the same as, or (2) opposite to that required by the target letter, or (3) did not require a response in this task. No evidence was found to support differentially greater response slowing by the elderly due to the presence of irrelevant stimuli. Elderly persons showed significantly less effects of response competition, suggesting that the two groups processed the task information differently. The results suggest that age differences in effects of perceptual noise and response competition are not general phenomena, but depend upon the demand characteristics of the specific task."}
{"id": "PMID:469190", "title": "Attribution for success and failure as a function of perceived age.", "content": "Based on an attributional model of achievement-related behavior, it was predicted that success on the part of a young person and failure on the part of an old person, being expected outcomes, would be attributed to stable causes, whereas success on the part of an old person and failure on the part of a young person, being unexpected outcomes, would be attributed to unstable causes. Using a between-subjects experimental design, subjects were asked to estimate the probability that a young vs an old stimulus person would succeed in college and to judge the causes for his success or failure. Although there occurred no differences as a function of age in expectancy of success or attributions for success, failure on the part of the old stimulus person was attributed more to the stable causes of lack of ability and task difficulty, while failure on the part of the young stimulus person was attributed more to the unstable cause of lack of effort. The results were discussed in terms of those conditions under which negative stereotypes concerning the competency of the elderly will be considered appropriate and thereby exert an influence on evaluations.", "contents": "Attribution for success and failure as a function of perceived age. Based on an attributional model of achievement-related behavior, it was predicted that success on the part of a young person and failure on the part of an old person, being expected outcomes, would be attributed to stable causes, whereas success on the part of an old person and failure on the part of a young person, being unexpected outcomes, would be attributed to unstable causes. Using a between-subjects experimental design, subjects were asked to estimate the probability that a young vs an old stimulus person would succeed in college and to judge the causes for his success or failure. Although there occurred no differences as a function of age in expectancy of success or attributions for success, failure on the part of the old stimulus person was attributed more to the stable causes of lack of ability and task difficulty, while failure on the part of the young stimulus person was attributed more to the unstable cause of lack of effort. The results were discussed in terms of those conditions under which negative stereotypes concerning the competency of the elderly will be considered appropriate and thereby exert an influence on evaluations."}
{"id": "PMID:469191", "title": "Psychic and somatic symptoms of depression among young adults, institutionalized aged and noninstitutionalized aged.", "content": "Beck Depression Inventory scores were obtained from 48 elderly who had been residing in homes for the aged for more than one year, 31 elderly residing in the community and waiting to enter an old-age home, and 424 young adults enrolled in a fist-year psychology course. The residents of old-age homes reported no more symptoms of depression than the waiting-list controls, a finding that provides no support for the hypothesis that the institional nature of old-age homes increases depression in the elderly. Both the institutionalized and noninstitutionalized aged reported more somatic symptoms of depression than the young adults, but no greater cognitive or affective symptoms of depression. These results were interpreted as providing no support for the widely belief that the aged are more depressed than any other age group. Finally, it was argued that somatic complaints can be valid indicators of depression in the elderly if normative differences between young and old are taken into account.", "contents": "Psychic and somatic symptoms of depression among young adults, institutionalized aged and noninstitutionalized aged. Beck Depression Inventory scores were obtained from 48 elderly who had been residing in homes for the aged for more than one year, 31 elderly residing in the community and waiting to enter an old-age home, and 424 young adults enrolled in a fist-year psychology course. The residents of old-age homes reported no more symptoms of depression than the waiting-list controls, a finding that provides no support for the hypothesis that the institional nature of old-age homes increases depression in the elderly. Both the institutionalized and noninstitutionalized aged reported more somatic symptoms of depression than the young adults, but no greater cognitive or affective symptoms of depression. These results were interpreted as providing no support for the widely belief that the aged are more depressed than any other age group. Finally, it was argued that somatic complaints can be valid indicators of depression in the elderly if normative differences between young and old are taken into account."}
{"id": "PMID:469192", "title": "Factors related to attitude towards retirement among French preretired managers and top executives.", "content": "This study aimed to investigate the determinants of a favorable attitude towards retirement among 175 male executives aged 63 or 64 and still professionally active. Findings were based on a questionnaire for attitude as well as a variety of other examinations designed to reveal the determinants involved. Multiple regression analysis indicated that four possible determinants are significantly related to a favorable attitude toward retirement: a good cardiovascular status, a poor state of vision, a high degree of life satisfaction and an important amount of time devoted to leisure activities. The relationship between commitment to work and attitude towards retirement was not found to be significant.", "contents": "Factors related to attitude towards retirement among French preretired managers and top executives. This study aimed to investigate the determinants of a favorable attitude towards retirement among 175 male executives aged 63 or 64 and still professionally active. Findings were based on a questionnaire for attitude as well as a variety of other examinations designed to reveal the determinants involved. Multiple regression analysis indicated that four possible determinants are significantly related to a favorable attitude toward retirement: a good cardiovascular status, a poor state of vision, a high degree of life satisfaction and an important amount of time devoted to leisure activities. The relationship between commitment to work and attitude towards retirement was not found to be significant."}
{"id": "PMID:469193", "title": "Wage determination and discrimination among older workers.", "content": "In this study, the determinants of the wage rates of a large sample of individuals aged 58 to 63 are first analyzed. Second, an explanation for the large discrepancies existing between the average wage rates earned by whites and nonwhites and by men and women is attempted. Human capital and geographic variables were found to be important wage determinants. Education, vocational training, years of job tenure, health, region of residence and local cost of living were significant explanators, especially for whites. Differences in these variables, however, cannot completely explain the wage differentials that exist by race and sex. There is a large unexplained component (especially in the male-female comparison) offering evidence of race and sex discrimination among older workers. In the case of sex discrimination, much of the problem appears to be occupational segregation--the crowding of women into low paying industries and occupations.", "contents": "Wage determination and discrimination among older workers. In this study, the determinants of the wage rates of a large sample of individuals aged 58 to 63 are first analyzed. Second, an explanation for the large discrepancies existing between the average wage rates earned by whites and nonwhites and by men and women is attempted. Human capital and geographic variables were found to be important wage determinants. Education, vocational training, years of job tenure, health, region of residence and local cost of living were significant explanators, especially for whites. Differences in these variables, however, cannot completely explain the wage differentials that exist by race and sex. There is a large unexplained component (especially in the male-female comparison) offering evidence of race and sex discrimination among older workers. In the case of sex discrimination, much of the problem appears to be occupational segregation--the crowding of women into low paying industries and occupations."}
{"id": "PMID:469194", "title": "Going home: aged return migration in the United States 1965-1970.", "content": "Two related issues concerning aged return migration are explored in this paper. They are: (1) the common features of states that strongly attract their migration-prone native sons and daughters, and (2) what might account for the differences, if any, in the demographic and socioeconomic characteristics of return and non-return migrants. Individual characteristics of persons, age 60 and over, from the 1970 1-in-100 Public Use Sample of Basic Records were analyzed. A cluster of Sunbelt states, and Utah, strongly attract return migrants; otherwise they tend to be attracted by states which are popular destinations for aged migration in general. Using discriminant function analysis and tests for mean differences of migrant characteristics, it was found that return migrants tend to be negatively selected on socioeconomic characteristics relative to other interstate migrants. Comparisons of migrant characteristics in streams from New York and Ohio to Florida with counterstreams from Florida to New York and Ohio were consistent with a model of secondary post-retirement moves to state of birth when service and social support needs increase.", "contents": "Going home: aged return migration in the United States 1965-1970. Two related issues concerning aged return migration are explored in this paper. They are: (1) the common features of states that strongly attract their migration-prone native sons and daughters, and (2) what might account for the differences, if any, in the demographic and socioeconomic characteristics of return and non-return migrants. Individual characteristics of persons, age 60 and over, from the 1970 1-in-100 Public Use Sample of Basic Records were analyzed. A cluster of Sunbelt states, and Utah, strongly attract return migrants; otherwise they tend to be attracted by states which are popular destinations for aged migration in general. Using discriminant function analysis and tests for mean differences of migrant characteristics, it was found that return migrants tend to be negatively selected on socioeconomic characteristics relative to other interstate migrants. Comparisons of migrant characteristics in streams from New York and Ohio to Florida with counterstreams from Florida to New York and Ohio were consistent with a model of secondary post-retirement moves to state of birth when service and social support needs increase."}
{"id": "PMID:469195", "title": "Relative deprivation and perception of financial adequacy among the aged.", "content": "This paper presents a conceptual framework that incorporates the notion of relative deprivation to explain the perceived financial adequacy among the elderly. The model not only provides a more heuristic interpretation of older people's financial satisfaction but also further clarifies the role of actual income. The relative deprivation model was evaluated by using data from the General Social Survey conducted by the National Opinion Research Ctr. from 1972 through 1977. The findings support the predictions derived from the model and were replicated with high consistency across these six data sets. According to the findings, financial satisfaction is directly related to relative deprivation while income only indirectly affects one's sense of financial well-being. Social status, income, and labor force participation have been demonstrated to be either directly or indirectly related to feelings of deprivation.", "contents": "Relative deprivation and perception of financial adequacy among the aged. This paper presents a conceptual framework that incorporates the notion of relative deprivation to explain the perceived financial adequacy among the elderly. The model not only provides a more heuristic interpretation of older people's financial satisfaction but also further clarifies the role of actual income. The relative deprivation model was evaluated by using data from the General Social Survey conducted by the National Opinion Research Ctr. from 1972 through 1977. The findings support the predictions derived from the model and were replicated with high consistency across these six data sets. According to the findings, financial satisfaction is directly related to relative deprivation while income only indirectly affects one's sense of financial well-being. Social status, income, and labor force participation have been demonstrated to be either directly or indirectly related to feelings of deprivation."}
{"id": "PMID:469196", "title": "[A histological and hormonal study of 399 women near the menopause or with confirmed menopause (author's transl)].", "content": "This work is concerned with 399 women who were either near the menopause or had had the menopause and on whom 476 hormone levels and 169 examinations of the endometrium had been carried out. It is possible to put the women into three groups. 13 p. 100 showed a diphasic cycle, with more or less normal ovarian function. Among the group of women whose cycle was not diphasic an appreciable number had normal oestrogenic activity, or were indeed hyperoestrogenic. The others were hypo-oestrogenic. Several facts can be derived from this study. Raised levels of E.2 can be found even when there is no cyclical activity and even in women who have raised levels of FSH. Normal ovarian function may be resumed several months after the menopause, although when the levels of FSH are raised the occurrence of the postmenopausal state would seem to be confirmed. The presence of hot flushes does not seem to correspond to a particular hormonal state. Marked differences seem to exist between the levels of the plasma hormones and the endometrial biopsy results. It is possible to have an atrophic endometrium with a raised level of oestradiol-17 beta, or on the other hand a polypoidal endometrium in women who are hypo-oestrogenic. The presence of levels of progesterone such as are found in a normal luteal phase does not indicate that the endometrium will necessarily be secretory. These contradictions cannot be resolved by studying the intra-cellular concentrations of the hormone receptors. The approach of the menopause is not always characterized by a progressive and continuous state of oestrogen deficiency or by hypophysial overactivity. This classical picture can be heavily shaded. All kinds of hormone profiles and responses by the receptor organs may be met and the clinician should be aware of these facts when he chooses therapy.", "contents": "[A histological and hormonal study of 399 women near the menopause or with confirmed menopause (author's transl)]. This work is concerned with 399 women who were either near the menopause or had had the menopause and on whom 476 hormone levels and 169 examinations of the endometrium had been carried out. It is possible to put the women into three groups. 13 p. 100 showed a diphasic cycle, with more or less normal ovarian function. Among the group of women whose cycle was not diphasic an appreciable number had normal oestrogenic activity, or were indeed hyperoestrogenic. The others were hypo-oestrogenic. Several facts can be derived from this study. Raised levels of E.2 can be found even when there is no cyclical activity and even in women who have raised levels of FSH. Normal ovarian function may be resumed several months after the menopause, although when the levels of FSH are raised the occurrence of the postmenopausal state would seem to be confirmed. The presence of hot flushes does not seem to correspond to a particular hormonal state. Marked differences seem to exist between the levels of the plasma hormones and the endometrial biopsy results. It is possible to have an atrophic endometrium with a raised level of oestradiol-17 beta, or on the other hand a polypoidal endometrium in women who are hypo-oestrogenic. The presence of levels of progesterone such as are found in a normal luteal phase does not indicate that the endometrium will necessarily be secretory. These contradictions cannot be resolved by studying the intra-cellular concentrations of the hormone receptors. The approach of the menopause is not always characterized by a progressive and continuous state of oestrogen deficiency or by hypophysial overactivity. This classical picture can be heavily shaded. All kinds of hormone profiles and responses by the receptor organs may be met and the clinician should be aware of these facts when he chooses therapy."}
{"id": "PMID:469197", "title": "[Myocardial necrosis due to tocolysis with fenoterol (author's transl)].", "content": "95 women had undergone treatment with beta-adrenergic Fenoterol for periods lasting as long as 9 weeks in order to try to avoid premature labour. Electrocardiographic studies and regular controls of the kinase isoenzymes of creatin which is specific for cardiac muscle were carried out in order to avoid myocardial damage, as was MB creatinkinase. In no case was a pathological rise in the activity of the enzyme shown even after full doses of the substance had been given for a long time.", "contents": "[Myocardial necrosis due to tocolysis with fenoterol (author's transl)]. 95 women had undergone treatment with beta-adrenergic Fenoterol for periods lasting as long as 9 weeks in order to try to avoid premature labour. Electrocardiographic studies and regular controls of the kinase isoenzymes of creatin which is specific for cardiac muscle were carried out in order to avoid myocardial damage, as was MB creatinkinase. In no case was a pathological rise in the activity of the enzyme shown even after full doses of the substance had been given for a long time."}
{"id": "PMID:469198", "title": "[Early repeated abortions and karyotypes. A cytogenic study of 80 consecutive couples (author's transl)].", "content": "The authors report the results of a cystogenic study of 80 couples (160 individuals) who had early repeated abortions. A significant karyotype abnormality was found in 5 per cent of the partners. Where a translocation of 2 heterological chromosomes is found in a partner, the recommendations is to attempt an antenatal diagnostic. Once a couple have had 2 abortions karyotype investigation is absolutely indicated.", "contents": "[Early repeated abortions and karyotypes. A cytogenic study of 80 consecutive couples (author's transl)]. The authors report the results of a cystogenic study of 80 couples (160 individuals) who had early repeated abortions. A significant karyotype abnormality was found in 5 per cent of the partners. Where a translocation of 2 heterological chromosomes is found in a partner, the recommendations is to attempt an antenatal diagnostic. Once a couple have had 2 abortions karyotype investigation is absolutely indicated."}
{"id": "PMID:469205", "title": "Congenital hypoplasia of the thumb with absence of the extrinsic extensors, abductor pollicis longus, and thenar muscles.", "content": "An unusual congenital anomaly of the thumb has been seen in 10 hands of eight patients. It consists of skeletal hypoplasia, instability of the metacarpophalangeal joint, absence of the extrinsic extensors and abductor, and adduction contracture of the first web space. Surgical reconstruction consisted of transfer of the extensor indicis proprius, the palmaris longus, and the abductor digiti minimi, as well as ankylosis of the metacarpophalangeal joint and release of the web space contracture. Useful grasp and pinch were provided for all patients. The patients were followed from 2 to 5 years.", "contents": "Congenital hypoplasia of the thumb with absence of the extrinsic extensors, abductor pollicis longus, and thenar muscles. An unusual congenital anomaly of the thumb has been seen in 10 hands of eight patients. It consists of skeletal hypoplasia, instability of the metacarpophalangeal joint, absence of the extrinsic extensors and abductor, and adduction contracture of the first web space. Surgical reconstruction consisted of transfer of the extensor indicis proprius, the palmaris longus, and the abductor digiti minimi, as well as ankylosis of the metacarpophalangeal joint and release of the web space contracture. Useful grasp and pinch were provided for all patients. The patients were followed from 2 to 5 years."}
{"id": "PMID:469199", "title": "[Contact embryoscopy (author's transl)].", "content": "Prenatal diagnosis based on anatomic definition of the fetus in utero is an difficult task, in the first trimester of pregnancy. The authors have recently introduced a new method : contact embryoscopy which consist in applying the optic piece of a contact hysteroscope (diameter of six millemeters) to the intact and translucent ovular membranes by the cervix. They have performed fifty embryoscopy prior to termination of pregnancy at 8 to 12 weeks. In 41 cases, they have seen the hands of fetus with the fingers. If this technic fails, it is possible, later, to use Echography and fetoscopy. The side-effects are discussed. Contact embryoscopy would be particularly useful in the detection of malformations of the extremities, for instance : chondro-ectodermal dysplasia (Ellis Van Creveld Syndrome) or ectrodactily.", "contents": "[Contact embryoscopy (author's transl)]. Prenatal diagnosis based on anatomic definition of the fetus in utero is an difficult task, in the first trimester of pregnancy. The authors have recently introduced a new method : contact embryoscopy which consist in applying the optic piece of a contact hysteroscope (diameter of six millemeters) to the intact and translucent ovular membranes by the cervix. They have performed fifty embryoscopy prior to termination of pregnancy at 8 to 12 weeks. In 41 cases, they have seen the hands of fetus with the fingers. If this technic fails, it is possible, later, to use Echography and fetoscopy. The side-effects are discussed. Contact embryoscopy would be particularly useful in the detection of malformations of the extremities, for instance : chondro-ectodermal dysplasia (Ellis Van Creveld Syndrome) or ectrodactily."}
{"id": "PMID:469200", "title": "[Teratospermia consisting of the absence of the head of the spermatozoa because of a fault in the joint between the head and the neck of the sperm in man (author's transl)].", "content": "An ultrastructural sperm study was conducted in a sterile man whose sperm count showed 100 percent headless spermatozoa. Testicular biopsy showed abnormal spermatogenesis : within the spermatid, the sperm head developed separately from the flagellum, which alone was ejected during spermiation.", "contents": "[Teratospermia consisting of the absence of the head of the spermatozoa because of a fault in the joint between the head and the neck of the sperm in man (author's transl)]. An ultrastructural sperm study was conducted in a sterile man whose sperm count showed 100 percent headless spermatozoa. Testicular biopsy showed abnormal spermatogenesis : within the spermatid, the sperm head developed separately from the flagellum, which alone was ejected during spermiation."}
{"id": "PMID:469206", "title": "Congenital radial head dislocation.", "content": "Fifty patients with 77 congenitally dislocated radial heads were reviewed to assess the diagnostic features, functional limitations, symptomatology, and results of treatment. Twenty-three patients had associated anomalies of the affected upper extremity, and 35 had anomalies in other parts of the body. In six patients no other anomaly was found, all six had bilateral involvement. No patient had congenital radial head dislocation as a unilateral isolated anomaly, and therefore we doubt its existence. Functional limitation with congenital radial head dislocation is minimal and pain is uncommon. Radial head excision relieves pain and improves appearance: however, it does not increase motion.", "contents": "Congenital radial head dislocation. Fifty patients with 77 congenitally dislocated radial heads were reviewed to assess the diagnostic features, functional limitations, symptomatology, and results of treatment. Twenty-three patients had associated anomalies of the affected upper extremity, and 35 had anomalies in other parts of the body. In six patients no other anomaly was found, all six had bilateral involvement. No patient had congenital radial head dislocation as a unilateral isolated anomaly, and therefore we doubt its existence. Functional limitation with congenital radial head dislocation is minimal and pain is uncommon. Radial head excision relieves pain and improves appearance: however, it does not increase motion."}
{"id": "PMID:469207", "title": "Vascular anatomy of flexor tendons. I. Vincular system and blood supply of the profundus tendon in the digital sheath.", "content": "The detailed blood supply of the flexor tendons in the digital canal was studied in 35 fresh human hands by means of an India ink-latex vascular injection technique. The specimens were examined by first exposing the pulley system, followed by examination of the intact tendon which had been rendered transparent by immersion in a solution of tributyl and tricresyl phosphate. Five annular and three cruciform pulleys were shown. There were five types of long vincula to the profundus (VLP) and three types of long vincula to the superficialis (VLS). The vincular systems of index and little fingers were symmetrical. Occasionally, neither the VLP nor the VLS was found in either the long or the ring fingers. The vincula received blood supply from four transverse branches of the digital arteries. A volar avascular area of the profundus tendon was seen in the cleared cross-sections, and the cross-over zone of its intrinsic vessels was found to be at the midproximal phalanx.", "contents": "Vascular anatomy of flexor tendons. I. Vincular system and blood supply of the profundus tendon in the digital sheath. The detailed blood supply of the flexor tendons in the digital canal was studied in 35 fresh human hands by means of an India ink-latex vascular injection technique. The specimens were examined by first exposing the pulley system, followed by examination of the intact tendon which had been rendered transparent by immersion in a solution of tributyl and tricresyl phosphate. Five annular and three cruciform pulleys were shown. There were five types of long vincula to the profundus (VLP) and three types of long vincula to the superficialis (VLS). The vincular systems of index and little fingers were symmetrical. Occasionally, neither the VLP nor the VLS was found in either the long or the ring fingers. The vincula received blood supply from four transverse branches of the digital arteries. A volar avascular area of the profundus tendon was seen in the cleared cross-sections, and the cross-over zone of its intrinsic vessels was found to be at the midproximal phalanx."}
{"id": "PMID:469201", "title": "[Lack of the central pair of axial filaments in a case of short-tailed spermatozoa human teratospermia (author's transl)].", "content": "A case of short-tailed human spermatozoa is presented in a patient with severe teratospermia. The middle piece was generally short, showing abundant cytoplasmic droplets, disruption of the mitochondrial sheath and absence of some coarsed fibers. The majority of spermatozoa showed enlargement of the principal piece, reduplication of proliferation of the fibrous sheath, and disorganization as well of the axial filament complex as of the coarsed fibers. An abnormal great number of microtubules and coarsed fibers were frequently randomly scattered throughout the cytoplasmic droplet. A further abnormality, consisting in the lack of the central pair of axial filaments was relevant in a 70 p. 100 of spermatozoa. This latter abnormality could be or not associated with the former alterations.", "contents": "[Lack of the central pair of axial filaments in a case of short-tailed spermatozoa human teratospermia (author's transl)]. A case of short-tailed human spermatozoa is presented in a patient with severe teratospermia. The middle piece was generally short, showing abundant cytoplasmic droplets, disruption of the mitochondrial sheath and absence of some coarsed fibers. The majority of spermatozoa showed enlargement of the principal piece, reduplication of proliferation of the fibrous sheath, and disorganization as well of the axial filament complex as of the coarsed fibers. An abnormal great number of microtubules and coarsed fibers were frequently randomly scattered throughout the cytoplasmic droplet. A further abnormality, consisting in the lack of the central pair of axial filaments was relevant in a 70 p. 100 of spermatozoa. This latter abnormality could be or not associated with the former alterations."}
{"id": "PMID:469208", "title": "Determination of muscle-tendon unit properties during tendon transfer.", "content": "Data on passive lengthening and active shortening from electrical stimulation to give a total functional excursion are presented. Length-tension characteristics of certain muscles used for transfer are given. Electrical stimulation of the newly transferred tendon gives useful information that is reproducible. This new knowledge obtained at operation is an important adjunct to the traditional techniques and provides helpful information in performing better procedures.", "contents": "Determination of muscle-tendon unit properties during tendon transfer. Data on passive lengthening and active shortening from electrical stimulation to give a total functional excursion are presented. Length-tension characteristics of certain muscles used for transfer are given. Electrical stimulation of the newly transferred tendon gives useful information that is reproducible. This new knowledge obtained at operation is an important adjunct to the traditional techniques and provides helpful information in performing better procedures."}
{"id": "PMID:469203", "title": "[The value of cis-diamminedichloroplatinum (cis-platinum) CDDP in the treatment of stage III and IV cancers of the ovary (author's transl)].", "content": "Cis-diamminedichloroplatinum is remarkably useful in stage III and IV cancers of the ovary. It should therefore be added to the armamentarium of chemotherapeutic substances used up till now. This effectiveness adds extra indication to the use of chemotherapy as a first treatment for stage III cancers of the ovary after the lesions have been reduced as far as possible by surgery.", "contents": "[The value of cis-diamminedichloroplatinum (cis-platinum) CDDP in the treatment of stage III and IV cancers of the ovary (author's transl)]. Cis-diamminedichloroplatinum is remarkably useful in stage III and IV cancers of the ovary. It should therefore be added to the armamentarium of chemotherapeutic substances used up till now. This effectiveness adds extra indication to the use of chemotherapy as a first treatment for stage III cancers of the ovary after the lesions have been reduced as far as possible by surgery."}
{"id": "PMID:469202", "title": "[Comparative action of an impeded estrogen, zeranol, and estradiol on some receptors in the female rat (author's transl)].", "content": "We have studied the estrogenic action of zeranol and have compared it to the action of estradiol on the uterus--the vagina, the mammary gland and the pituitary gland of the pre-pubescent, castrated female before the opening of the vagina. The weakest dose at which estradiol is active is 0.1 microgram injected for a period of three days : it is capable of opening the vagina on the fourth or fifth day and of developping the uterine glands to the maximum : injected over a period of ten days it provokes a hypertrophy of the pituitary gland : only the mammary gland reacts exclusively to doses other than physiological ones. The estrogenic action of zeranol is weak : 1 mg administered orally for three days is necessary in order to reproduce the same effects of 0,1 microgram of estradiol on the opening and the keratinisation of the vagina and on the stimulation of the uterine epithelium ; its efficacy is greater on the uterine glands (250 micrograms is equivalent to 0.1 microgram) and on the pituitary gland (500 microgram reproduce the hypertrophy provoked by 0,1 microgram). The doses administered are by 100 g of body weight. The uterine glands are more sensitive to stimulation by the estrogens employed (estradiol, zeranol) than is the uterine epithelium. The study of the relation between the estrogenic dose and the response of the uterine weight shows that the gradient of the curve is weaker with zeranol than with estradiol. This observation shows that zeranol behaves as an impeded estrogen.", "contents": "[Comparative action of an impeded estrogen, zeranol, and estradiol on some receptors in the female rat (author's transl)]. We have studied the estrogenic action of zeranol and have compared it to the action of estradiol on the uterus--the vagina, the mammary gland and the pituitary gland of the pre-pubescent, castrated female before the opening of the vagina. The weakest dose at which estradiol is active is 0.1 microgram injected for a period of three days : it is capable of opening the vagina on the fourth or fifth day and of developping the uterine glands to the maximum : injected over a period of ten days it provokes a hypertrophy of the pituitary gland : only the mammary gland reacts exclusively to doses other than physiological ones. The estrogenic action of zeranol is weak : 1 mg administered orally for three days is necessary in order to reproduce the same effects of 0,1 microgram of estradiol on the opening and the keratinisation of the vagina and on the stimulation of the uterine epithelium ; its efficacy is greater on the uterine glands (250 micrograms is equivalent to 0.1 microgram) and on the pituitary gland (500 microgram reproduce the hypertrophy provoked by 0,1 microgram). The doses administered are by 100 g of body weight. The uterine glands are more sensitive to stimulation by the estrogens employed (estradiol, zeranol) than is the uterine epithelium. The study of the relation between the estrogenic dose and the response of the uterine weight shows that the gradient of the curve is weaker with zeranol than with estradiol. This observation shows that zeranol behaves as an impeded estrogen."}
{"id": "PMID:469209", "title": "The mechanism of dorsal fracture dislocation of the fifth carpometacarpal joint.", "content": "Fracture dislocation at the base of the fifth metacarpal is a rare injury. Fewer than 25 cases have been published in the English-language literature. A force acting along the longitudinal axis of the fifth metacarpal causes a fracture dislocation at the base of the bone in a dorsal-ulnar direction, because of the anatomy of the joint and the mechanical properties of the bone and the intermetacarpal ligament. The mechanism of other types of injury to this joint, namely dorsal-radial or palmar-radial dislocation of the base of the fifth metacarpal is different. A case is presented which demonstrates the mechanism of the fracture dislocation.", "contents": "The mechanism of dorsal fracture dislocation of the fifth carpometacarpal joint. Fracture dislocation at the base of the fifth metacarpal is a rare injury. Fewer than 25 cases have been published in the English-language literature. A force acting along the longitudinal axis of the fifth metacarpal causes a fracture dislocation at the base of the bone in a dorsal-ulnar direction, because of the anatomy of the joint and the mechanical properties of the bone and the intermetacarpal ligament. The mechanism of other types of injury to this joint, namely dorsal-radial or palmar-radial dislocation of the base of the fifth metacarpal is different. A case is presented which demonstrates the mechanism of the fracture dislocation."}
{"id": "PMID:469204", "title": "[Tobacco and pregnancy (author's transl)].", "content": "The evil effects of tobacco on the health of those who smoke it have been emphasized broadly in the past few years, both in the public and in the medical press. The possible damage that can be done to those who are \"passive\" smokers has also been pointed out. Now the fetus is in the front row of these. That is why it is important to point out this fact. After a brief statement about the physio-pathology of the condition the authors review the literature and extract the following points which can be ascribed to the use of tobacco: -- lowering of the birth weight; -- considerable increase in risk of premature separation of the placenta; -- a negative correlation with the frequency of pre-eclamptic toxaemia. The authors point out the additional feature that the effect of tobacco can potentiate the effects of an associated pathological condition. Finally, they emphasize the methodological difficulties which account for the numerous discrepancies and uncertainties that still exist about the action of tobacco on pregnancy.", "contents": "[Tobacco and pregnancy (author's transl)]. The evil effects of tobacco on the health of those who smoke it have been emphasized broadly in the past few years, both in the public and in the medical press. The possible damage that can be done to those who are \"passive\" smokers has also been pointed out. Now the fetus is in the front row of these. That is why it is important to point out this fact. After a brief statement about the physio-pathology of the condition the authors review the literature and extract the following points which can be ascribed to the use of tobacco: -- lowering of the birth weight; -- considerable increase in risk of premature separation of the placenta; -- a negative correlation with the frequency of pre-eclamptic toxaemia. The authors point out the additional feature that the effect of tobacco can potentiate the effects of an associated pathological condition. Finally, they emphasize the methodological difficulties which account for the numerous discrepancies and uncertainties that still exist about the action of tobacco on pregnancy."}
{"id": "PMID:469211", "title": "Rupture of the radial collateral ligament of a metacarpophalangeal joint to one of the ulnar three fingers.", "content": "In six patients rupture of the radial collateral ligament of the metacarpophalangeal joint of one of the three ulnar fingers, surgical reconstruction was successful using local tissues in five and a tendon graft in one. Operative treatment of this injury is indicated only when significant instability is present.", "contents": "Rupture of the radial collateral ligament of a metacarpophalangeal joint to one of the ulnar three fingers. In six patients rupture of the radial collateral ligament of the metacarpophalangeal joint of one of the three ulnar fingers, surgical reconstruction was successful using local tissues in five and a tendon graft in one. Operative treatment of this injury is indicated only when significant instability is present."}
{"id": "PMID:469212", "title": "Mechanical analysis of Kirschner wire fixation in a phalangeal model.", "content": "Transverse divisions of the mid-shaft of freshly frozen pig metacarpals were fixed with Kirschner wires of two sizes and using four different configurations. Compared to the usual cross-pin fixation using wires of 0.889 mm. four wires (0.712 mm) eccentrically placed and with their ends hooked provided a 300% improvement in bending rigidity and 170% in bending movement.", "contents": "Mechanical analysis of Kirschner wire fixation in a phalangeal model. Transverse divisions of the mid-shaft of freshly frozen pig metacarpals were fixed with Kirschner wires of two sizes and using four different configurations. Compared to the usual cross-pin fixation using wires of 0.889 mm. four wires (0.712 mm) eccentrically placed and with their ends hooked provided a 300% improvement in bending rigidity and 170% in bending movement."}
{"id": "PMID:469213", "title": "External fixator use in comminuted gunshot fractures of the proximal phalanx.", "content": "Comminuted low velocity gunshot fractures of the proximal phalanx were stabilized with an external fixator in 15 patients. This device was found to be useful. It allowed healing of the fracture in good alignment in 13 patients. And motion of the metacarpophalangeal joint was preserved.", "contents": "External fixator use in comminuted gunshot fractures of the proximal phalanx. Comminuted low velocity gunshot fractures of the proximal phalanx were stabilized with an external fixator in 15 patients. This device was found to be useful. It allowed healing of the fracture in good alignment in 13 patients. And motion of the metacarpophalangeal joint was preserved."}
{"id": "PMID:469215", "title": "Epitheloid sarcoma: a case report.", "content": "Epitheloid sarcoma of the pulp of the thumb followed repeated penetrating injuries to the digit. It was treated aggressively and successfully to date, nearly 5 years later. In some respects this case report parallels those animal experiments in which foreign body tumorigenesis has been demonstrated, and one might entertain a correlation.", "contents": "Epitheloid sarcoma: a case report. Epitheloid sarcoma of the pulp of the thumb followed repeated penetrating injuries to the digit. It was treated aggressively and successfully to date, nearly 5 years later. In some respects this case report parallels those animal experiments in which foreign body tumorigenesis has been demonstrated, and one might entertain a correlation."}
{"id": "PMID:469218", "title": "Heparin-induced thromboembolism: a case report.", "content": "A case of heparin-induced thrombosis of the brachial artery with thrombocytopenia is described. With discontinuation of the heparin, the circulating platelet volumes returned to normal. The role of pre-existing arterial disease and sepsis in patients exhibiting this phenomenon is unresolved.", "contents": "Heparin-induced thromboembolism: a case report. A case of heparin-induced thrombosis of the brachial artery with thrombocytopenia is described. With discontinuation of the heparin, the circulating platelet volumes returned to normal. The role of pre-existing arterial disease and sepsis in patients exhibiting this phenomenon is unresolved."}
{"id": "PMID:469220", "title": "Cataract--webbed Peromyscus. I. Genetics of cataract in P. maniculatus.", "content": "Cataract--webbed (cw) in the deer mouse (Peromyscus maniculatus) is a recessive mutant that is manifested by syndactyly (involving soft tissues) and eye defects including cataract. Within cw/cw mice, the age at which cataract first appears is genetically controlled. Evidence for the activity of genetic modifiers is the early appearance of cataracts in the offspring of parents that developed cataracts at early ages.", "contents": "Cataract--webbed Peromyscus. I. Genetics of cataract in P. maniculatus. Cataract--webbed (cw) in the deer mouse (Peromyscus maniculatus) is a recessive mutant that is manifested by syndactyly (involving soft tissues) and eye defects including cataract. Within cw/cw mice, the age at which cataract first appears is genetically controlled. Evidence for the activity of genetic modifiers is the early appearance of cataracts in the offspring of parents that developed cataracts at early ages."}
{"id": "PMID:469222", "title": "Allozyme variability and relatedness in six crayfish species.", "content": "A total of twenty-six allozyme loci were assayed in six crayfish species. Estimated mean heterozygosity was low (5.5 percent) for all species in comparison with the invertebrate average. The specific values, however, ranged from 2.9 percent to 8.3 percent. These crayfish estimates are consistent with estimates for other decapods where low heterozygosity is the rule. The consistency of low heterozygosity estimates in the order Decapoda suggests that its explanation may include both order specific differences such as low mutation or intracistronic recombination rates and species specific differences. A dendrogram, constructed using Nei's D, is in agreement with the morphological clustering.", "contents": "Allozyme variability and relatedness in six crayfish species. A total of twenty-six allozyme loci were assayed in six crayfish species. Estimated mean heterozygosity was low (5.5 percent) for all species in comparison with the invertebrate average. The specific values, however, ranged from 2.9 percent to 8.3 percent. These crayfish estimates are consistent with estimates for other decapods where low heterozygosity is the rule. The consistency of low heterozygosity estimates in the order Decapoda suggests that its explanation may include both order specific differences such as low mutation or intracistronic recombination rates and species specific differences. A dendrogram, constructed using Nei's D, is in agreement with the morphological clustering."}
{"id": "PMID:469223", "title": "Green shanks and adult mortality in chickens.", "content": "A mutation of recent origin in Cornell randombred Leghorn chickens causes the shanks to be colored green due to melanin-like deposition in the dermis, in contrast to normal yellow. Some pigment is often present in the mandibles and the mature feathers display an ashen cast. This mutant is determined by an allele, idc, on the sex chromosome. Excessive adult mortality with a high incidence of tumors, mainly hemangiomas, is associated with this gene--at least extending over a two-generation observation interval. Pullets receiving this mutant allele (idc) from their sire die at 2 to 3 times the rate of sisters, with yellow shanks, that carry the Id allele.", "contents": "Green shanks and adult mortality in chickens. A mutation of recent origin in Cornell randombred Leghorn chickens causes the shanks to be colored green due to melanin-like deposition in the dermis, in contrast to normal yellow. Some pigment is often present in the mandibles and the mature feathers display an ashen cast. This mutant is determined by an allele, idc, on the sex chromosome. Excessive adult mortality with a high incidence of tumors, mainly hemangiomas, is associated with this gene--at least extending over a two-generation observation interval. Pullets receiving this mutant allele (idc) from their sire die at 2 to 3 times the rate of sisters, with yellow shanks, that carry the Id allele."}
{"id": "PMID:469224", "title": "Esterase variation at three loci in meat ants.", "content": "The meat ant (Iridomyrmex purpureus) occurs in a number of color forms, with uncertain taxonomic status. Gel electrophresis of meat ant extracts, followed by nonspecific esterase staining, reveals several zones of activity. Allelic variation at three loci is proposed to account for variation in some of these zones. Two of the loci (Es-1, Es-2) appear to have recessive null alleles, whose frequencies have been estimated by the method of maximum likelihood. Geographic variation in allele frequency is attributed to behavioral and geographic subdivision of the population. Apparent disturbances in segregation ratios and deviations from Hardy-Weinberg equilibrium can be accounted for if it is argued that some nests contain more than one queen. Differences in gene frequency between sympatric populations of the red and blue forms of I. purpureus are observed, confirming their reproductive is isolation and sibling species status.", "contents": "Esterase variation at three loci in meat ants. The meat ant (Iridomyrmex purpureus) occurs in a number of color forms, with uncertain taxonomic status. Gel electrophresis of meat ant extracts, followed by nonspecific esterase staining, reveals several zones of activity. Allelic variation at three loci is proposed to account for variation in some of these zones. Two of the loci (Es-1, Es-2) appear to have recessive null alleles, whose frequencies have been estimated by the method of maximum likelihood. Geographic variation in allele frequency is attributed to behavioral and geographic subdivision of the population. Apparent disturbances in segregation ratios and deviations from Hardy-Weinberg equilibrium can be accounted for if it is argued that some nests contain more than one queen. Differences in gene frequency between sympatric populations of the red and blue forms of I. purpureus are observed, confirming their reproductive is isolation and sibling species status."}
{"id": "PMID:469225", "title": "Identification of a 16--17-day lethal factor in a chondrodystrophic stock of turkeys.", "content": "Chondrodystrophy in turkeys is a lethal condition produced by an autosomal allele, ch. Mutants are characterized by very short and thick legs, shortened wings, globular head with parrot beak, and a protruding abdomen. Classification of ch/ch embryos may be accurately made at 12 days incubation, prior to which limited appendicular development precludes distinction from normal or heterozygous birds. Death caused by the mutant allele occurs between 24 and 28 days; some embryos are alive but unpipped at time of hatch. Analysis of the pattern of embryonic mortality for the stock carrying the ch allele revealed at 16--17-day lethal factor that appears to be independent of chondrodystrophy. Of 52 females mated to Ch/ch males, 41 were determined to be Ch/ch; 32 of these heterozygous females exhibited normal embryonic mortality patterns. Nine hens, in addition to being Ch/ch, displayed a significant increase in embryo death between 15 and 18 days, peaking sharply at 17 days. Of the 11 females determined to be Ch/Ch, five showed the same 15 to 18 day pattern of mortality observed for the nine Ch/ch females.", "contents": "Identification of a 16--17-day lethal factor in a chondrodystrophic stock of turkeys. Chondrodystrophy in turkeys is a lethal condition produced by an autosomal allele, ch. Mutants are characterized by very short and thick legs, shortened wings, globular head with parrot beak, and a protruding abdomen. Classification of ch/ch embryos may be accurately made at 12 days incubation, prior to which limited appendicular development precludes distinction from normal or heterozygous birds. Death caused by the mutant allele occurs between 24 and 28 days; some embryos are alive but unpipped at time of hatch. Analysis of the pattern of embryonic mortality for the stock carrying the ch allele revealed at 16--17-day lethal factor that appears to be independent of chondrodystrophy. Of 52 females mated to Ch/ch males, 41 were determined to be Ch/ch; 32 of these heterozygous females exhibited normal embryonic mortality patterns. Nine hens, in addition to being Ch/ch, displayed a significant increase in embryo death between 15 and 18 days, peaking sharply at 17 days. Of the 11 females determined to be Ch/Ch, five showed the same 15 to 18 day pattern of mortality observed for the nine Ch/ch females."}
{"id": "PMID:469231", "title": "Intracellular labeling with ferritin conjugates. A specificity problem due to the affinity of unconjugated ferritin for selected intracellular sites.", "content": "Nonspecific binding of ferritin to chromatin and the cytoplasmic aspect of the nuclear envelope was observed when nonantigenic, serum-washed hepatocyte nuclei were incubated in ferritin-antibody conjugates. This labeling was duplicated when nuclei from a wide range of species and cell types were exposed to unconjugated ferritin. Unconjugated ferritin binding to nuclei did not depend on a subpopulation of denatured molecules or on the ferritin purification procedure. Binding occurred equally on unfixed and formaldehyde-fixed nuclei, but no ferritin bound to glutaraldehyde-fixed nuclei. Inconjugated ferritin also bound to the cytoplasmic aspects of the rough endoplasmic reticulum and the plasma membrane. The tracer did not bind to lysosomes, mitochondria, Golgi vesicles, the extracellular surface of plasma membranes, or the intracisternal surfaces of ruptured nuclear envelopes. The addition of 0.4 M KCl or 0.7 M NaCl to ferritin solutions and washing media at neutral pH reduced the binding of conjugated and unconjugated ferritin to nuclei to about 3% of that seen in 0.10 M phosphate buffer alone. The added salts caused little extraction of nuclear contents from formaldehyde-fixed nuclei. The use of one of these salts in ferritin conjugates should considerably improve the specificity of intracellular labeling.", "contents": "Intracellular labeling with ferritin conjugates. A specificity problem due to the affinity of unconjugated ferritin for selected intracellular sites. Nonspecific binding of ferritin to chromatin and the cytoplasmic aspect of the nuclear envelope was observed when nonantigenic, serum-washed hepatocyte nuclei were incubated in ferritin-antibody conjugates. This labeling was duplicated when nuclei from a wide range of species and cell types were exposed to unconjugated ferritin. Unconjugated ferritin binding to nuclei did not depend on a subpopulation of denatured molecules or on the ferritin purification procedure. Binding occurred equally on unfixed and formaldehyde-fixed nuclei, but no ferritin bound to glutaraldehyde-fixed nuclei. Inconjugated ferritin also bound to the cytoplasmic aspects of the rough endoplasmic reticulum and the plasma membrane. The tracer did not bind to lysosomes, mitochondria, Golgi vesicles, the extracellular surface of plasma membranes, or the intracisternal surfaces of ruptured nuclear envelopes. The addition of 0.4 M KCl or 0.7 M NaCl to ferritin solutions and washing media at neutral pH reduced the binding of conjugated and unconjugated ferritin to nuclei to about 3% of that seen in 0.10 M phosphate buffer alone. The added salts caused little extraction of nuclear contents from formaldehyde-fixed nuclei. The use of one of these salts in ferritin conjugates should considerably improve the specificity of intracellular labeling."}
{"id": "PMID:469227", "title": "Desired family size and sex of children in Botucatu, Brazil.", "content": "During 1977--78, 715 unmarried college students at the Universidade Estadual Paulista, Botucatu, Brazil, were surveyed for their preferences for family sizes and combinations and permutations of sexes of children. The most preferred family size by both male and female respondents was two children. A slightly lower proportion of respondents preferred a family size of three children. Average size of the desired families was approximately 2.76 children. The most preferred combinations of sexes were one male, one female; one female, two males; and two females, two males, respectively, in families of two, three, and four children. Preferred permutations of sexes were those in which the first child was male and subsequent children resulted in an alternation of sexes.", "contents": "Desired family size and sex of children in Botucatu, Brazil. During 1977--78, 715 unmarried college students at the Universidade Estadual Paulista, Botucatu, Brazil, were surveyed for their preferences for family sizes and combinations and permutations of sexes of children. The most preferred family size by both male and female respondents was two children. A slightly lower proportion of respondents preferred a family size of three children. Average size of the desired families was approximately 2.76 children. The most preferred combinations of sexes were one male, one female; one female, two males; and two females, two males, respectively, in families of two, three, and four children. Preferred permutations of sexes were those in which the first child was male and subsequent children resulted in an alternation of sexes."}
{"id": "PMID:469233", "title": "A technique for assessing nutritional 'at-risk' factors in residential homes for the elderly.", "content": "A technique for assessing the degree of nutritional risk to residents in old people's homes is outlined. Administrative, psychological and social factors are considered as well as food. Twenty-six risk factors are identified. In one short visit, using questionnaires to the matron, cook and the residents and a check-list of general observations, the degree of risk, high, moderate or low, can be assessed. The technique has been used in a pilot study for comparisons between homes, and for advice to individual homes.", "contents": "A technique for assessing nutritional 'at-risk' factors in residential homes for the elderly. A technique for assessing the degree of nutritional risk to residents in old people's homes is outlined. Administrative, psychological and social factors are considered as well as food. Twenty-six risk factors are identified. In one short visit, using questionnaires to the matron, cook and the residents and a check-list of general observations, the degree of risk, high, moderate or low, can be assessed. The technique has been used in a pilot study for comparisons between homes, and for advice to individual homes."}
{"id": "PMID:469228", "title": "Assignment of the H-Y antigen gene to the short arm of chromosome Y.", "content": "We have presented two cases strongly suporting a Y chromosome short-arm location for the H-Y antigen gene. The first case was HY antigen-positive with an isochromosome for the short arm of the Y with no long arm of the Y being present. The other case was H-Y antigen-negative in fibroblasts from an individual with a 46,X,i(Yq) karyotype with no short arm of the Y present. The two cases presented also confirmed previous reports that the testicular forming gene is also located on the short arm of chromosome Y.", "contents": "Assignment of the H-Y antigen gene to the short arm of chromosome Y. We have presented two cases strongly suporting a Y chromosome short-arm location for the H-Y antigen gene. The first case was HY antigen-positive with an isochromosome for the short arm of the Y with no long arm of the Y being present. The other case was H-Y antigen-negative in fibroblasts from an individual with a 46,X,i(Yq) karyotype with no short arm of the Y present. The two cases presented also confirmed previous reports that the testicular forming gene is also located on the short arm of chromosome Y."}
{"id": "PMID:469234", "title": "Portion sizes, preparation and cooking yields, and plate waste in school meals.", "content": "Available portion sizes for a wide range of menu items were measured in seven junior schools and three senior schools in one local authority area. Serving waste was also measured and used as a criterion of acceptability. Portion sizes for entrees, desserts and potatoes were generally acceptable except for a few unpopular items. Custard was the most acceptable milky sauce. A small range of vegetables was acceptable in small quantities, but excess was rejected while others were almost totally rejected. Peeling, trimming and cooking yields for vegatables, trimming and cooking yields for meat, and plate waste were measured over one year in 180 schools in eight local authority areas. Results for vegetables compared favourably with previously published data  except for new potatoes where the yield after machine peeling was lower. Cooking yields for pork, beef and lamb were slightly lower than previously published values. Trimming waste from meat for stewing was substantially greater than the 5 per cent visible fat which is the catering specification for ordering. The mean plate waste was 33 g per child meal (excluding bones).", "contents": "Portion sizes, preparation and cooking yields, and plate waste in school meals. Available portion sizes for a wide range of menu items were measured in seven junior schools and three senior schools in one local authority area. Serving waste was also measured and used as a criterion of acceptability. Portion sizes for entrees, desserts and potatoes were generally acceptable except for a few unpopular items. Custard was the most acceptable milky sauce. A small range of vegetables was acceptable in small quantities, but excess was rejected while others were almost totally rejected. Peeling, trimming and cooking yields for vegatables, trimming and cooking yields for meat, and plate waste were measured over one year in 180 schools in eight local authority areas. Results for vegetables compared favourably with previously published data  except for new potatoes where the yield after machine peeling was lower. Cooking yields for pork, beef and lamb were slightly lower than previously published values. Trimming waste from meat for stewing was substantially greater than the 5 per cent visible fat which is the catering specification for ordering. The mean plate waste was 33 g per child meal (excluding bones)."}
{"id": "PMID:469235", "title": "Food preferences of some United Kingdom residents.", "content": "A food preference questionnaire was completed by 1304 people. There were considerable differences in food preferences related to age, sex, and area of residence of the respondents. In spite of these differences, five distinct clusters of foods showing similar preference patterns were indentified. These clusters were of sweet fruits, meats, vegetables, alcoholic beverages, and some high carbohydrate foods. The cause of the clustering was uncertain.", "contents": "Food preferences of some United Kingdom residents. A food preference questionnaire was completed by 1304 people. There were considerable differences in food preferences related to age, sex, and area of residence of the respondents. In spite of these differences, five distinct clusters of foods showing similar preference patterns were indentified. These clusters were of sweet fruits, meats, vegetables, alcoholic beverages, and some high carbohydrate foods. The cause of the clustering was uncertain."}
{"id": "PMID:469229", "title": "Fertile dominant spotting in the house mouse.", "content": "Recognition of a new allele (Wf) at the W locus of the mouse is based on genetic analysis and demonstration of defective hair pigmentation, macrocytic anemia, and defects in pluripotent hemopoietic stem cells. Unlike other alleles of the W series, this one does not result in sterility.", "contents": "Fertile dominant spotting in the house mouse. Recognition of a new allele (Wf) at the W locus of the mouse is based on genetic analysis and demonstration of defective hair pigmentation, macrocytic anemia, and defects in pluripotent hemopoietic stem cells. Unlike other alleles of the W series, this one does not result in sterility."}
{"id": "PMID:469236", "title": "An interview model for use in dietetic training.", "content": "Student dietitians commonly encounter difficulties with interviewing patients. These difficulties may arise through ignorance of either the function of the interview or the interview technique. Some major problems are described and an interview model is proposed as one method of helping the student to cope.", "contents": "An interview model for use in dietetic training. Student dietitians commonly encounter difficulties with interviewing patients. These difficulties may arise through ignorance of either the function of the interview or the interview technique. Some major problems are described and an interview model is proposed as one method of helping the student to cope."}
{"id": "PMID:469237", "title": "A national nutrition policy: can we devise one?", "content": "The reasons for the success of the British food and nutritional policy of the second world war are discussed. Subsequent trends in Britain and elsewhere towards the affluent pattern of diet are described and the current views about diet and heart disease and diet and cancer are summarised. Conclusions are drawn about the need for public education on the modification of diet towards amore prudent pattern. The terms food policy and nutritional policy are distinguished.", "contents": "A national nutrition policy: can we devise one? The reasons for the success of the British food and nutritional policy of the second world war are discussed. Subsequent trends in Britain and elsewhere towards the affluent pattern of diet are described and the current views about diet and heart disease and diet and cancer are summarised. Conclusions are drawn about the need for public education on the modification of diet towards amore prudent pattern. The terms food policy and nutritional policy are distinguished."}
{"id": "PMID:469240", "title": "The impact of OSHA inspections on manufacturing injury rates.", "content": "This paper estimates the effects of OSHA inspections on injury rates at the plant level. The methodology involves comparing injury rates of plants inspected 'early'in a given year with those inspected \"late\" in the year, after controlling for prior injury rates and other factors. The results suggest that OSHA inspections in 1973 reduced injury rates by about 16 percent, but that 1974 inspections had no statistically significant impact.", "contents": "The impact of OSHA inspections on manufacturing injury rates. This paper estimates the effects of OSHA inspections on injury rates at the plant level. The methodology involves comparing injury rates of plants inspected 'early'in a given year with those inspected \"late\" in the year, after controlling for prior injury rates and other factors. The results suggest that OSHA inspections in 1973 reduced injury rates by about 16 percent, but that 1974 inspections had no statistically significant impact."}
{"id": "PMID:469243", "title": "The ability of the murine placenta to absorb monoclonal anti-fetal H-2K antibody from the maternal circulation.", "content": "In previous work we have found that partially purified 125I-labeled anti H-2 antibody is localized in the placenta when injected i.v. into females pregnant by males bearing the target haplotype. This led to the concept that the placenta is an H-2 antigen-bearing immunoabsorbent barrier between mother and fetus. In this report we have used an anti-H-2Kk monoclonal antibody of the IgG2a subclass, also labeled with 125I, to verify this concept, as well as to improve the genetic definition of the immunoabsorbent antigen. In addition we have prepared F(ab')2 fragments of this antibody, and these also show the immunoabsorbent effect. This indicates that transport into fetally derived tissues via Fc binding is not a prerequisite for reaction of the antibody with paternal strain H-2 K antigens.", "contents": "The ability of the murine placenta to absorb monoclonal anti-fetal H-2K antibody from the maternal circulation. In previous work we have found that partially purified 125I-labeled anti H-2 antibody is localized in the placenta when injected i.v. into females pregnant by males bearing the target haplotype. This led to the concept that the placenta is an H-2 antigen-bearing immunoabsorbent barrier between mother and fetus. In this report we have used an anti-H-2Kk monoclonal antibody of the IgG2a subclass, also labeled with 125I, to verify this concept, as well as to improve the genetic definition of the immunoabsorbent antigen. In addition we have prepared F(ab')2 fragments of this antibody, and these also show the immunoabsorbent effect. This indicates that transport into fetally derived tissues via Fc binding is not a prerequisite for reaction of the antibody with paternal strain H-2 K antigens."}
{"id": "PMID:469244", "title": "Binding constants of NZB myeloma antidextrans for dextrans and isomaltose oligosaccharides determined by affinity electrophoresis.", "content": "Association constants of dextrans (Ka) and oligosaccharides (Kia) from NZB myeloma antidextrans (PC3858 and PC3936) were studied by affinity electrophoresis. With linear dextrans or with those with a low degree of branching, Ka ranged from 2.7 X 10(3) to 5.4 X 10(4) ml/g for PC3858 and from 1.3 X 10(4) to 2.6 X 10(5) ml/g for PC3936. Completely linear alpha-(1 leads to 6)-linked dextrans, LD7 and D3, showed relatively high affinities for the two NZB antidextrans. With oligosaccharides, the Kia value increased as the number oa alpha-(1 leads to 6)-linked glycosyl residues increased. Isomaltoheptaose (IM7) showed the highest Kia (1.9 X 10(4) M-1 for PC3858 and 1.63 X 10(4) M-1 for PC3936), whereas isomaltose (IM2) had the lowest Kia (2.36 X 10(2)M-1 for PC3858 and 1.32 X 10(2)M-1 for PC3936). Pullulan and glycogen showed very weak affinity for PC3936, but they did not react at all with PC3858. These findings indicate that NZB myeloma antidextrans, PC3858 and PC3936, are specific for internal chains of alpha-(1 leads to 6)-linked dextrans. Data on the precision with which Ka and Kia can be determined are presented.", "contents": "Binding constants of NZB myeloma antidextrans for dextrans and isomaltose oligosaccharides determined by affinity electrophoresis. Association constants of dextrans (Ka) and oligosaccharides (Kia) from NZB myeloma antidextrans (PC3858 and PC3936) were studied by affinity electrophoresis. With linear dextrans or with those with a low degree of branching, Ka ranged from 2.7 X 10(3) to 5.4 X 10(4) ml/g for PC3858 and from 1.3 X 10(4) to 2.6 X 10(5) ml/g for PC3936. Completely linear alpha-(1 leads to 6)-linked dextrans, LD7 and D3, showed relatively high affinities for the two NZB antidextrans. With oligosaccharides, the Kia value increased as the number oa alpha-(1 leads to 6)-linked glycosyl residues increased. Isomaltoheptaose (IM7) showed the highest Kia (1.9 X 10(4) M-1 for PC3858 and 1.63 X 10(4) M-1 for PC3936), whereas isomaltose (IM2) had the lowest Kia (2.36 X 10(2)M-1 for PC3858 and 1.32 X 10(2)M-1 for PC3936). Pullulan and glycogen showed very weak affinity for PC3936, but they did not react at all with PC3858. These findings indicate that NZB myeloma antidextrans, PC3858 and PC3936, are specific for internal chains of alpha-(1 leads to 6)-linked dextrans. Data on the precision with which Ka and Kia can be determined are presented."}
{"id": "PMID:469247", "title": "The release of a platelet-activating factor by stimulated rabbit neutrophils.", "content": "Normal rabbit peripheral blood neutrophils released a platelet-activating factor upon stimulation by opsonized zymosan. The liberation was Ca++ dependent and the time course of release was closely associated with phagocytosis. The material extracted into chloroform and exhibited an identical mobility by thin layer chromatography to basophil-derived, IgE-stimulated, platelet-activating factor (PAFb). It was similar to PAFb in its effect on platelets in both aggregation and release but was distinguished from ADP, thrombin, arachidonic acid, and thromboxanes. This factor appears to be responsible for some previously reported neutrophil-platelet interactions.", "contents": "The release of a platelet-activating factor by stimulated rabbit neutrophils. Normal rabbit peripheral blood neutrophils released a platelet-activating factor upon stimulation by opsonized zymosan. The liberation was Ca++ dependent and the time course of release was closely associated with phagocytosis. The material extracted into chloroform and exhibited an identical mobility by thin layer chromatography to basophil-derived, IgE-stimulated, platelet-activating factor (PAFb). It was similar to PAFb in its effect on platelets in both aggregation and release but was distinguished from ADP, thrombin, arachidonic acid, and thromboxanes. This factor appears to be responsible for some previously reported neutrophil-platelet interactions."}
{"id": "PMID:469250", "title": "Suppression of cell-mediated tumor cell lysis and complement-induced cytotoxicity by trypan blue.", "content": "Different forms of cell-mediated cytotoxicity were suppressed in the presence of trypan blue. The systems affected included lysis of antibody-coated tumor cells by normal and C. parvum-stimulated mouse peritoneal cells and lysis of allogeneic targets by immune effector cells. The inhibition, measured in a 4-hr 51Cr release assay, was reversible and did not occur in the presence of 30% fetal calf serum or albumin. Binding between effector and target cells through Fc receptors was not affected, and lysis of allogeneic cells was inhibited at the lytic step rather than at the binding step. In contrast, lysis of sensitized erythrocytes was not inhibited by trypan blue, suggesting that lysis of these targets may not involve the steps required in tumor cell lysis. Trypan blue blocked the function of antibody before binding to target cells and also suppressed complement-induced cytolysis. Most individual complement components were susceptible to the inhibitory action of trypan blue. These results reveal an affinity of trypan blue for proteins in general that may be responsible for many of its biologic actions.", "contents": "Suppression of cell-mediated tumor cell lysis and complement-induced cytotoxicity by trypan blue. Different forms of cell-mediated cytotoxicity were suppressed in the presence of trypan blue. The systems affected included lysis of antibody-coated tumor cells by normal and C. parvum-stimulated mouse peritoneal cells and lysis of allogeneic targets by immune effector cells. The inhibition, measured in a 4-hr 51Cr release assay, was reversible and did not occur in the presence of 30% fetal calf serum or albumin. Binding between effector and target cells through Fc receptors was not affected, and lysis of allogeneic cells was inhibited at the lytic step rather than at the binding step. In contrast, lysis of sensitized erythrocytes was not inhibited by trypan blue, suggesting that lysis of these targets may not involve the steps required in tumor cell lysis. Trypan blue blocked the function of antibody before binding to target cells and also suppressed complement-induced cytolysis. Most individual complement components were susceptible to the inhibitory action of trypan blue. These results reveal an affinity of trypan blue for proteins in general that may be responsible for many of its biologic actions."}
{"id": "PMID:469251", "title": "Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b.", "content": "Antibody-dependent cytolytic effector lymphocytes are known to possess, in part, receptors for activated C3. Employing a model system consisting of 51Cr-labeled chicken erythrocytes and purified human peripheral lymphocytes, we investigated the effect of target cell bound C3b on antibody-dependent cellular cytotoxicity (ADCC). At concentrations of anti-target cell antibody too low to cause effective ADCC, target cell bound C3b cooperated with antibody to produce marked target cell lysis. In the presence of a 1/6.25 X 10(6) dilution of anti-chicken erythrocyte rabbit IgG, cell lysis increased from 20% to 65% by the attachment of 18,000 C3b molecules per cell. C3b-dependent enhancement of ADCC was dose dependent. It was augmented by attachment of activated properdin (P) to the C3b-bearing target cells.", "contents": "Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b. Antibody-dependent cytolytic effector lymphocytes are known to possess, in part, receptors for activated C3. Employing a model system consisting of 51Cr-labeled chicken erythrocytes and purified human peripheral lymphocytes, we investigated the effect of target cell bound C3b on antibody-dependent cellular cytotoxicity (ADCC). At concentrations of anti-target cell antibody too low to cause effective ADCC, target cell bound C3b cooperated with antibody to produce marked target cell lysis. In the presence of a 1/6.25 X 10(6) dilution of anti-chicken erythrocyte rabbit IgG, cell lysis increased from 20% to 65% by the attachment of 18,000 C3b molecules per cell. C3b-dependent enhancement of ADCC was dose dependent. It was augmented by attachment of activated properdin (P) to the C3b-bearing target cells."}
{"id": "PMID:469252", "title": "Cyclophosphamide and cortisone acetate inhibit complement biosynthesis by guinea pig bronchoalveolar macrophages.", "content": "To explore mechanisms of drug-induced alterations in local host defenses in the lung, the capacity of guinea pig alveolar macrophages to synthesize the second (C2) and fourth (C4) components of complement was studied following treatment with cyclophosphamide and cortisone acetate. Administration of either drug significantly inhibited biosynthesis of C2 and C4 within 1 day. After 1 week of treatment, local complement synthesis was inhibited approximately 80%, although serum levels of the corresponding proteins were normal.", "contents": "Cyclophosphamide and cortisone acetate inhibit complement biosynthesis by guinea pig bronchoalveolar macrophages. To explore mechanisms of drug-induced alterations in local host defenses in the lung, the capacity of guinea pig alveolar macrophages to synthesize the second (C2) and fourth (C4) components of complement was studied following treatment with cyclophosphamide and cortisone acetate. Administration of either drug significantly inhibited biosynthesis of C2 and C4 within 1 day. After 1 week of treatment, local complement synthesis was inhibited approximately 80%, although serum levels of the corresponding proteins were normal."}
{"id": "PMID:469254", "title": "Passive enhancement of mouse tumor allografts by alloantibodies is Fc-dependent.", "content": "Enhancement of growth of a B10.D2 fibrosarcoma in B6AF1 recipients could be induced by administration of B6AF1 anti-B10.D2 lymphocyte serum. The role of the Fc part in this phenomenon was studied by treatment of the B6AF1 recipients with F(ab')2 fragments of enhancing alloantibodies. A highly purified F(ab')2 preparation was used to exclude any effects of undigested IgG. Administration of F(ab')2 did not lead to enhancement of the tumor allografts, not even when given in a dose that was 22 times the molar amount of the lowest enhancing dose of undigested IgG. We therefore conclude that passive enhancement of mouse tumor allografts by alloantibodies is Fc dependent.", "contents": "Passive enhancement of mouse tumor allografts by alloantibodies is Fc-dependent. Enhancement of growth of a B10.D2 fibrosarcoma in B6AF1 recipients could be induced by administration of B6AF1 anti-B10.D2 lymphocyte serum. The role of the Fc part in this phenomenon was studied by treatment of the B6AF1 recipients with F(ab')2 fragments of enhancing alloantibodies. A highly purified F(ab')2 preparation was used to exclude any effects of undigested IgG. Administration of F(ab')2 did not lead to enhancement of the tumor allografts, not even when given in a dose that was 22 times the molar amount of the lowest enhancing dose of undigested IgG. We therefore conclude that passive enhancement of mouse tumor allografts by alloantibodies is Fc dependent."}
{"id": "PMID:469256", "title": "The enzymatic nature of human c1r: a subcomponent of the first component of complement.", "content": "The esterase activity of the C1r subcomponent of the first component of complement has been investigated. C1r was found to hydrolyze two amino acid methyl esters; N-acetyl-L-arginine methyl ester and N-acetyl-glycyl-L-lysine methyl ester, and two amino acid p-nitrophenyl esters, N-carbobenzyloxy-L-tyrosine-p-nitrophenyl ester and N alpha-carbobenzyloxy-L-lysine-p-nitrophenyl ester. A detailed kinetic analysis of the hydrolysis of N-Z-L-Tyr-ONp by C1r revealed that the enzymatic activity per microgram of protein decreased as the C1r concentration was increased. The loss of activity suggested that above 0.5 micron C1r was undergoing aggregation with a loss of active sites. Similarly, when C1r was titrated with the active site titrant p-nitrophenyl-P'-guanidinobenzoate the number of titratable sites per milligram of protein decreased with increasing protein concentration. The hydrolysis of N-Z-L-Tyr-ONp by C1r was inhibited by several synthetic inhibitors including phenylmethanesulfonylfluoride, p-amidinophenylmethanesulfonylfluoride, diisopropylfluorophosphate, and p-tosyl-L-lysine-chloromethyl ketone. However, the peptide esterase inhibitors Trasylol, hirudin, leupeptin, and C1 esterase inhibitor had no effect on the esterase activity of C1r.", "contents": "The enzymatic nature of human c1r: a subcomponent of the first component of complement. The esterase activity of the C1r subcomponent of the first component of complement has been investigated. C1r was found to hydrolyze two amino acid methyl esters; N-acetyl-L-arginine methyl ester and N-acetyl-glycyl-L-lysine methyl ester, and two amino acid p-nitrophenyl esters, N-carbobenzyloxy-L-tyrosine-p-nitrophenyl ester and N alpha-carbobenzyloxy-L-lysine-p-nitrophenyl ester. A detailed kinetic analysis of the hydrolysis of N-Z-L-Tyr-ONp by C1r revealed that the enzymatic activity per microgram of protein decreased as the C1r concentration was increased. The loss of activity suggested that above 0.5 micron C1r was undergoing aggregation with a loss of active sites. Similarly, when C1r was titrated with the active site titrant p-nitrophenyl-P'-guanidinobenzoate the number of titratable sites per milligram of protein decreased with increasing protein concentration. The hydrolysis of N-Z-L-Tyr-ONp by C1r was inhibited by several synthetic inhibitors including phenylmethanesulfonylfluoride, p-amidinophenylmethanesulfonylfluoride, diisopropylfluorophosphate, and p-tosyl-L-lysine-chloromethyl ketone. However, the peptide esterase inhibitors Trasylol, hirudin, leupeptin, and C1 esterase inhibitor had no effect on the esterase activity of C1r."}
{"id": "PMID:469259", "title": "A semi-automatic method of measuring leucocyte movement.", "content": "A semi-automatic system has been developed for measuring the migration of leucocytes within a micropore filter. The distance in micrometers is displayed digitally or analysed on-line by a digital computer. These improvements have resulted in considerable saving in time, permitting the full potential of the micropore method to be utilised. To illustrate this technique we report results from experiments to measure dose-response relationships of leucocytes to casein and the variation in response to Staphylococcus aureus and casein of cells from normal human subjects.", "contents": "A semi-automatic method of measuring leucocyte movement. A semi-automatic system has been developed for measuring the migration of leucocytes within a micropore filter. The distance in micrometers is displayed digitally or analysed on-line by a digital computer. These improvements have resulted in considerable saving in time, permitting the full potential of the micropore method to be utilised. To illustrate this technique we report results from experiments to measure dose-response relationships of leucocytes to casein and the variation in response to Staphylococcus aureus and casein of cells from normal human subjects."}
{"id": "PMID:469260", "title": "A capillary diffusion method for determining hemolytic complement activity in undiluted serum .", "content": "A method is presented for measuring the hemolytic activity of undiluted guinea pig serum. Undiluted serum is placed in contact with a column of sensitized sheep red blood cells in an agar matrix in a capillary tube. After incubation, the length of the zone of lysis is measured. Comparison with a standard curve prepared from dilutions of a pool of normal serum allows relative quantitation. Because the serum is not diluted, this method allows detection of reversible inhibitors of the complement system.", "contents": "A capillary diffusion method for determining hemolytic complement activity in undiluted serum . A method is presented for measuring the hemolytic activity of undiluted guinea pig serum. Undiluted serum is placed in contact with a column of sensitized sheep red blood cells in an agar matrix in a capillary tube. After incubation, the length of the zone of lysis is measured. Comparison with a standard curve prepared from dilutions of a pool of normal serum allows relative quantitation. Because the serum is not diluted, this method allows detection of reversible inhibitors of the complement system."}
{"id": "PMID:469261", "title": "The effect of label affinity on the sensitivity and specificity of a hapten radioimmunoassay: a comparison of three [125I]diphenylhydantoin radioligands with the 14C-labelled drug.", "content": "The effect on the sensitivity and specificity of a radioimmunoassay for diphenylhydantoin (DPH)has been investigated using three 125I-labelled tyrosine ester derivatives of DPH having different bridge lengths between the tyrosine moiety and the DPH moiety and 14C-labelled DPH. The results demonstrate that for a hapten which does not completely fill the antibody-binding sites, greatest sensitivity is achieved when the bridge of the iodine label is most dissimilar to that present in the original immunogen, when the hapten and label affinities are nearly equivalent. Greatest specificity is achieved with the label which most resembles the original immunogen. These results illustrate the difficulty of designing satisfactory labels for assays of both high specificity and sensitivity since minimal changes in label structure may produce greatly amplified changes in the subsequent affinity of the label for the antiserum.", "contents": "The effect of label affinity on the sensitivity and specificity of a hapten radioimmunoassay: a comparison of three [125I]diphenylhydantoin radioligands with the 14C-labelled drug. The effect on the sensitivity and specificity of a radioimmunoassay for diphenylhydantoin (DPH)has been investigated using three 125I-labelled tyrosine ester derivatives of DPH having different bridge lengths between the tyrosine moiety and the DPH moiety and 14C-labelled DPH. The results demonstrate that for a hapten which does not completely fill the antibody-binding sites, greatest sensitivity is achieved when the bridge of the iodine label is most dissimilar to that present in the original immunogen, when the hapten and label affinities are nearly equivalent. Greatest specificity is achieved with the label which most resembles the original immunogen. These results illustrate the difficulty of designing satisfactory labels for assays of both high specificity and sensitivity since minimal changes in label structure may produce greatly amplified changes in the subsequent affinity of the label for the antiserum."}
{"id": "PMID:469262", "title": "Purification of rodent eosinophils on discontinuous metrizamide gradients.", "content": "Methods are described for the purification of rat and mouse eosinophils. Peritoneal exudate cells obtained from Schistosoma mansoni- or Trichinella spiralis-infected mice were plated on plastic dishes, and the nonadherent cells were centrifuged over discontinuous hypertonic metrizamide gradients. Approximately 41% of those eosinophils present in the original preparations were recovered from the interfaces between 20% and 22% metrizamide at an average of 88% purity. Peritoneal cells obtained from either uninfected or S. mansoni-infected rats  were placed directly onto metrizamide gradients. After centrifugation, essentially all eosinophils present in the original preparations were recovered at the interface between 18.5% and 22.5% metrizamide, with an average of 80% purity. These methods for eosinophil purification have proven to be reproducible and to yield cells which are morphologically preserved and functional, as demonstrated by their ability to respond to a chemotactic stimulus.", "contents": "Purification of rodent eosinophils on discontinuous metrizamide gradients. Methods are described for the purification of rat and mouse eosinophils. Peritoneal exudate cells obtained from Schistosoma mansoni- or Trichinella spiralis-infected mice were plated on plastic dishes, and the nonadherent cells were centrifuged over discontinuous hypertonic metrizamide gradients. Approximately 41% of those eosinophils present in the original preparations were recovered from the interfaces between 20% and 22% metrizamide at an average of 88% purity. Peritoneal cells obtained from either uninfected or S. mansoni-infected rats  were placed directly onto metrizamide gradients. After centrifugation, essentially all eosinophils present in the original preparations were recovered at the interface between 18.5% and 22.5% metrizamide, with an average of 80% purity. These methods for eosinophil purification have proven to be reproducible and to yield cells which are morphologically preserved and functional, as demonstrated by their ability to respond to a chemotactic stimulus."}
{"id": "PMID:469263", "title": "Receptors for IgM on human lymphocytes. I. Detection of receptors on freshly drawn lymphocytes and at physiological temperature.", "content": "Receptors for the Fc portion of IgM (RFcmu) have previously been found to be primarily associated with the T lymphocyte subpopulation in humans, and their detection has frequently required overnight incubation of the cells before assay. In the present study, using highly sensitized indicator cells (EAmu), lymphocytes with receptor for IgM (EAmu-RFC) were detected immediately after isolation from human peripheral blood. Identification of EAmu-RFC on freshly isolated lymphocytes was greatly facilitated by assay at room temperature (RT) or 37 degrees C, implying that analogous interactions between IgM-antigen complexes and lymphocytes with receptor for IgM normally occur in vivo. These results seem to indicate that some RFcmu on lymphocytes may be occupied by IgM and/or IgM-antigen complexes in vivo and that immediately after isolation many RFcmu are still occupied and unavailable for interaction with EAmu. It is suggested that the successful competition of IgM-antigen complexes for RFcmu on T lymphocytes is the signal for and/or mechanism of expression of help by the lymphocyte. The sensitivity of the assay for EAmu-RFC also permitted the identification of a small subpopulation of RFcmu+ lymphocytes in the non-T cell fraction of the peripheral blood of some individuals.", "contents": "Receptors for IgM on human lymphocytes. I. Detection of receptors on freshly drawn lymphocytes and at physiological temperature. Receptors for the Fc portion of IgM (RFcmu) have previously been found to be primarily associated with the T lymphocyte subpopulation in humans, and their detection has frequently required overnight incubation of the cells before assay. In the present study, using highly sensitized indicator cells (EAmu), lymphocytes with receptor for IgM (EAmu-RFC) were detected immediately after isolation from human peripheral blood. Identification of EAmu-RFC on freshly isolated lymphocytes was greatly facilitated by assay at room temperature (RT) or 37 degrees C, implying that analogous interactions between IgM-antigen complexes and lymphocytes with receptor for IgM normally occur in vivo. These results seem to indicate that some RFcmu on lymphocytes may be occupied by IgM and/or IgM-antigen complexes in vivo and that immediately after isolation many RFcmu are still occupied and unavailable for interaction with EAmu. It is suggested that the successful competition of IgM-antigen complexes for RFcmu on T lymphocytes is the signal for and/or mechanism of expression of help by the lymphocyte. The sensitivity of the assay for EAmu-RFC also permitted the identification of a small subpopulation of RFcmu+ lymphocytes in the non-T cell fraction of the peripheral blood of some individuals."}
{"id": "PMID:469264", "title": "Activity of mouse macrophages purified by adherence to, and removal from, a plastic surface.", "content": "Mouse peritoneal exudate macrophages were allowed to adhere to plastic Petri dishes and, after washing, were removed by means of EDTA. Cells with the morphology of macrophages were recovered in a fair degree of purity (90-98%) but in low yield (31-34%). The macrophages recovered were fully active in the following ways: re-adherence to glass; pinocytosis of colloidal gold; phagocytosis of opsonized sheep erythrocytes; binding cytophilic antibody; production of lymphocyte activating factor; cytotoxic and cytostatic effects on tumour cells.", "contents": "Activity of mouse macrophages purified by adherence to, and removal from, a plastic surface. Mouse peritoneal exudate macrophages were allowed to adhere to plastic Petri dishes and, after washing, were removed by means of EDTA. Cells with the morphology of macrophages were recovered in a fair degree of purity (90-98%) but in low yield (31-34%). The macrophages recovered were fully active in the following ways: re-adherence to glass; pinocytosis of colloidal gold; phagocytosis of opsonized sheep erythrocytes; binding cytophilic antibody; production of lymphocyte activating factor; cytotoxic and cytostatic effects on tumour cells."}
{"id": "PMID:469265", "title": "Particle counting immunoassay (PACIA). III. Automated determination of circulating immune complexes by inhibition of an agglutinating factor of mouse serum.", "content": "A factor capable of agglutinating human IgG coated particles (latex) has been found in mouse serum. This factor (MAG) was used in an unpurified form to detect circulating immune complexes in the particle counting immunoassay (PACIA) system, which allows measurement of agglutination with great precision. MAG did not react with monomeric IgG, nor with reduced and alkylated aggregated IgG. It was inhibited by immune complexes in small antigen excess. Among the various subclasses of IgG, IgA and IgM, only IgG1 and IgM when coupled to Sepharose beads displayed an inhibitory activity towards MAG. That the inhibitory factors detected in serum were immune complexes or aggregated Ig was suggested by the correlation obtained with the amounts of 'heavy' IgG found in the serum samples by Ultrogel chromatography and by the polydisperse distribution of the inhibitory factors in the heavy fraction of gradient ultracentrifugation.", "contents": "Particle counting immunoassay (PACIA). III. Automated determination of circulating immune complexes by inhibition of an agglutinating factor of mouse serum. A factor capable of agglutinating human IgG coated particles (latex) has been found in mouse serum. This factor (MAG) was used in an unpurified form to detect circulating immune complexes in the particle counting immunoassay (PACIA) system, which allows measurement of agglutination with great precision. MAG did not react with monomeric IgG, nor with reduced and alkylated aggregated IgG. It was inhibited by immune complexes in small antigen excess. Among the various subclasses of IgG, IgA and IgM, only IgG1 and IgM when coupled to Sepharose beads displayed an inhibitory activity towards MAG. That the inhibitory factors detected in serum were immune complexes or aggregated Ig was suggested by the correlation obtained with the amounts of 'heavy' IgG found in the serum samples by Ultrogel chromatography and by the polydisperse distribution of the inhibitory factors in the heavy fraction of gradient ultracentrifugation."}
{"id": "PMID:469266", "title": "The occurrence and treatment of false positive reactions in enzyme-linked immunosorbent assays (ELISA) for the presence of fungal antigens in clinical samples.", "content": "Non-specific positive reactions have been revealed in enzyme-linked immunosorbent assays (ELISA) of sera for the presence of fungal antigen. These false positives were recognized by their occurrence in tests for both Candida albicans and Aspergillus fumigatus antigens and by their response to dithiothreitol, combined with their reaction with non-immune rabbit globulin. A scheme is proposed which differentiates between true and false positive reactions. Use of fractionated anti-fungal globulin in conjugates reduced the incidence of false positive results in sera from hospitalized patients and eliminated them from sera of healthy subjects. The test scheme was applied to two panels of sera containing samples from patients with (a) invasive candidosis and (b) invasive aspergillosis. The relevance of ELISA tests for the detection of fungal antigen in human serum is discussed.", "contents": "The occurrence and treatment of false positive reactions in enzyme-linked immunosorbent assays (ELISA) for the presence of fungal antigens in clinical samples. Non-specific positive reactions have been revealed in enzyme-linked immunosorbent assays (ELISA) of sera for the presence of fungal antigen. These false positives were recognized by their occurrence in tests for both Candida albicans and Aspergillus fumigatus antigens and by their response to dithiothreitol, combined with their reaction with non-immune rabbit globulin. A scheme is proposed which differentiates between true and false positive reactions. Use of fractionated anti-fungal globulin in conjugates reduced the incidence of false positive results in sera from hospitalized patients and eliminated them from sera of healthy subjects. The test scheme was applied to two panels of sera containing samples from patients with (a) invasive candidosis and (b) invasive aspergillosis. The relevance of ELISA tests for the detection of fungal antigen in human serum is discussed."}
{"id": "PMID:469267", "title": "A single-step immunization by sustained antigen release.", "content": "An inert polymer pellet less than 1 mm in diameter, implanted subcutaneously in mice, releases free antigen continuously and enhances antibody formation for over 6 months. The immune response stimulated by sustained antigen delivery is comparable to the secondary response induced by the same total dose of antigen emulsified in complete Freund's adjuvant. The sustained release polymer implants, using antigens over a wide molecular weight range, proved effective in eliciting prolonged antibody formation. The antigens tested included bovine serum albumin, gamma-globulin, and ribonuclease. Superior biocompatibility and release characteristics make the ethylene-vinyl acetate copolymer a promising method for the single-step induction of immunity.", "contents": "A single-step immunization by sustained antigen release. An inert polymer pellet less than 1 mm in diameter, implanted subcutaneously in mice, releases free antigen continuously and enhances antibody formation for over 6 months. The immune response stimulated by sustained antigen delivery is comparable to the secondary response induced by the same total dose of antigen emulsified in complete Freund's adjuvant. The sustained release polymer implants, using antigens over a wide molecular weight range, proved effective in eliciting prolonged antibody formation. The antigens tested included bovine serum albumin, gamma-globulin, and ribonuclease. Superior biocompatibility and release characteristics make the ethylene-vinyl acetate copolymer a promising method for the single-step induction of immunity."}
{"id": "PMID:469268", "title": "Particle counting immunoassay. IV. The use of F(ab')2 fragments and N epsilon-chloroacetyl lysine N-carboxyanhydride for their coupling to polystyrene latex particles.", "content": "Improved performance with the latex agglutination immunoassay, PACIA, is possible when the F(ab')2 fragments of antibody are chemically coupled at their hinge region to a protein-coated latex using a new coupling reagent, N epsilon-chloroacetyl lysine N-carboxyanhydride. This reagent, whether by orientating the complete antibody molecule or its F(ab')2 fragment or by preventing antibody denaturation, increased the sensitivity of the PACIA method. The use of F(ab')2 fragments eliminated most serum interference with the test.", "contents": "Particle counting immunoassay. IV. The use of F(ab')2 fragments and N epsilon-chloroacetyl lysine N-carboxyanhydride for their coupling to polystyrene latex particles. Improved performance with the latex agglutination immunoassay, PACIA, is possible when the F(ab')2 fragments of antibody are chemically coupled at their hinge region to a protein-coated latex using a new coupling reagent, N epsilon-chloroacetyl lysine N-carboxyanhydride. This reagent, whether by orientating the complete antibody molecule or its F(ab')2 fragment or by preventing antibody denaturation, increased the sensitivity of the PACIA method. The use of F(ab')2 fragments eliminated most serum interference with the test."}
{"id": "PMID:469269", "title": "A new method for measuring antibody using radiolabeled protein A1 in a solid-phase radioimmunoassay.", "content": "A micro solid-phase radioimmunoassay was developed which utilizes radiolabeled staphylococcal Protein A ([125I]Protein A) in place of radiolabeled anti-immunoglobulin ([125I]anti-IgG) for the measurement of antibody. For the assay, antigen is adsorbed to the wells of a microtiter plate followed by dilutions of serum and [125I]-Protein A in subsequent steps. We found that this assay can be used to measure antibody (Ab) against a variety of antigens in human and rabbit but not goat immune serum. Binding of [125I]Protein A and [125I]anti-IgG to human and rabbit IgG was comparable. It was possible to quantify this amount of Ab in human serum by reference to immune rabbit serum. The sensitivity of this assay for rabbit antibody was 1 ng/ml.", "contents": "A new method for measuring antibody using radiolabeled protein A1 in a solid-phase radioimmunoassay. A micro solid-phase radioimmunoassay was developed which utilizes radiolabeled staphylococcal Protein A ([125I]Protein A) in place of radiolabeled anti-immunoglobulin ([125I]anti-IgG) for the measurement of antibody. For the assay, antigen is adsorbed to the wells of a microtiter plate followed by dilutions of serum and [125I]-Protein A in subsequent steps. We found that this assay can be used to measure antibody (Ab) against a variety of antigens in human and rabbit but not goat immune serum. Binding of [125I]Protein A and [125I]anti-IgG to human and rabbit IgG was comparable. It was possible to quantify this amount of Ab in human serum by reference to immune rabbit serum. The sensitivity of this assay for rabbit antibody was 1 ng/ml."}
{"id": "PMID:469270", "title": "Determination of phenobarbital by radioimmunoassay.", "content": "A radioimmunoassay for phenobarbital has been studied. Antiphenobarbital antisera were obtained by repeated immunization of rabbits with p-succinamidophenobarbital conjugated to bovine serum albumin. Less than 0.2 pmol of phenobarbital could be measured by this procedure. The specificity of the antibodies was directed to substituents on the nitrogen atoms of the barbituric ring as well as to substituents at the carbon 5-position of the ring.", "contents": "Determination of phenobarbital by radioimmunoassay. A radioimmunoassay for phenobarbital has been studied. Antiphenobarbital antisera were obtained by repeated immunization of rabbits with p-succinamidophenobarbital conjugated to bovine serum albumin. Less than 0.2 pmol of phenobarbital could be measured by this procedure. The specificity of the antibodies was directed to substituents on the nitrogen atoms of the barbituric ring as well as to substituents at the carbon 5-position of the ring."}
{"id": "PMID:469271", "title": "Radioimmunoassay of glycosphingolipids: application for the detection of forssman glycolipid in tissue extracts and cell membranes.", "content": "Development of a radioimmunoassay for detecting glycosphingolipids has been difficult, primarily because of transfer of radiolabeled glycolipid antigen to the unlabeled antigen pool. This difficulty has been overcome by the use of a radiolabeled glycolipid-polymer. Thus, an assay system has been developed for measuring picomolar quantities of Forssman hapten glycolipid. This assay is based on competition for rabbit anti-Forssman antibodies between Forssman glycolipid and a radiolabeled Forssman polyacrylic hydrazide polymer. Antigen-antibody complexes are removed quickly and efficiently by binding to formalin-fixed Staphylococcus aureus and subsequent centrifugation. One nanogram of Forssman glycolipid can be readily detected both in plasma membrane preparations and in purified glycolipid fractions. The isoantigenic expression of Forssman glycolipid in human gastrointestinal tissues has been reported previously (Hakomori et al., 1977). Using the radioimmunoassay, the Forssman status of several additional cases has been determined quantitatively.", "contents": "Radioimmunoassay of glycosphingolipids: application for the detection of forssman glycolipid in tissue extracts and cell membranes. Development of a radioimmunoassay for detecting glycosphingolipids has been difficult, primarily because of transfer of radiolabeled glycolipid antigen to the unlabeled antigen pool. This difficulty has been overcome by the use of a radiolabeled glycolipid-polymer. Thus, an assay system has been developed for measuring picomolar quantities of Forssman hapten glycolipid. This assay is based on competition for rabbit anti-Forssman antibodies between Forssman glycolipid and a radiolabeled Forssman polyacrylic hydrazide polymer. Antigen-antibody complexes are removed quickly and efficiently by binding to formalin-fixed Staphylococcus aureus and subsequent centrifugation. One nanogram of Forssman glycolipid can be readily detected both in plasma membrane preparations and in purified glycolipid fractions. The isoantigenic expression of Forssman glycolipid in human gastrointestinal tissues has been reported previously (Hakomori et al., 1977). Using the radioimmunoassay, the Forssman status of several additional cases has been determined quantitatively."}
{"id": "PMID:469273", "title": "Dithiothreitol separation of newborn rodent dermis and epidermis.", "content": "Incubation of newborn mouse skin in media containing 0.01 M dithiothreitol at 4 degrees C for 2 hr reduces dermal-epidermal cohesion, and the 2 layers may be separated readily. Unlike other methods of separation, this technique does not require heating, nonphysiologic pH or ionic strength, or addition of proteolytic enzymes. The split occurs beneath the basal lamina. The separated epidermal sheet retains the periodic acid Schiff-positive \"basement membrane,\" and the basal cells can incorporate 3H-thymidine.", "contents": "Dithiothreitol separation of newborn rodent dermis and epidermis. Incubation of newborn mouse skin in media containing 0.01 M dithiothreitol at 4 degrees C for 2 hr reduces dermal-epidermal cohesion, and the 2 layers may be separated readily. Unlike other methods of separation, this technique does not require heating, nonphysiologic pH or ionic strength, or addition of proteolytic enzymes. The split occurs beneath the basal lamina. The separated epidermal sheet retains the periodic acid Schiff-positive \"basement membrane,\" and the basal cells can incorporate 3H-thymidine."}
{"id": "PMID:469274", "title": "Repair of ultraviolet light damage to the DNA of cultured human epidermal keratinocytes and fibroblasts.", "content": "Pure cultures of dermal fibroblasts and epidermal keratinocytes have been obtained from a single biopsy of newborn foreskin. The cells were labeled, exposed to several doses of UV light, and allowed to repair in the dark for 16 hr. The number of pyrimidine dimers before and after repair was assessed by measuring the numbers of sites in the DNA sensitive to a specific UV endonuclease. At all doses used, the extent of repair was similar in the cultured keratinocytes and cultured fibroblasts.", "contents": "Repair of ultraviolet light damage to the DNA of cultured human epidermal keratinocytes and fibroblasts. Pure cultures of dermal fibroblasts and epidermal keratinocytes have been obtained from a single biopsy of newborn foreskin. The cells were labeled, exposed to several doses of UV light, and allowed to repair in the dark for 16 hr. The number of pyrimidine dimers before and after repair was assessed by measuring the numbers of sites in the DNA sensitive to a specific UV endonuclease. At all doses used, the extent of repair was similar in the cultured keratinocytes and cultured fibroblasts."}
{"id": "PMID:469275", "title": "Comparison of lymphocyte function after isolation by ficoll-hypaque flotation or elutriation.", "content": "Mononuclear leukocytes were separated from whole blood by ficoll-hypaque flotation and by elutriation (counterflow centrifugation). Lymphocytes isolated from 6 control subjects by elutriation showed a 30% greater response to stimulation with phytohemagglutinin and 130% greater response to streptokinase-streptodornase stimulation than did autologous lymphocytes obtained by ficoll-hypaque separation. Cell yields of major mononuclear cell subpopulations and cell viability were comparable after separation of leukocytes by both techniques. These results indicate that ficoll-hypaque flotation may diminish lymphocyte responses, and that elutriation offers a useful alternative to ficoll-hypaque separation. In addition, elutriation may be the preferable method for evaluation of lymphocytes from patients with suspected immunologic dysfunction and may be valuable in the isolation of mononuclear cells from infiltrated skin lesions.", "contents": "Comparison of lymphocyte function after isolation by ficoll-hypaque flotation or elutriation. Mononuclear leukocytes were separated from whole blood by ficoll-hypaque flotation and by elutriation (counterflow centrifugation). Lymphocytes isolated from 6 control subjects by elutriation showed a 30% greater response to stimulation with phytohemagglutinin and 130% greater response to streptokinase-streptodornase stimulation than did autologous lymphocytes obtained by ficoll-hypaque separation. Cell yields of major mononuclear cell subpopulations and cell viability were comparable after separation of leukocytes by both techniques. These results indicate that ficoll-hypaque flotation may diminish lymphocyte responses, and that elutriation offers a useful alternative to ficoll-hypaque separation. In addition, elutriation may be the preferable method for evaluation of lymphocytes from patients with suspected immunologic dysfunction and may be valuable in the isolation of mononuclear cells from infiltrated skin lesions."}
{"id": "PMID:469276", "title": "Interstrand crosslinks in DNA of phage lambda after exposure to 8-methoxypsoralen and trimethylpsoralen in the presence of light.", "content": "Two medically useful photosensitizing furocoumarins, 8-methoxypsoralen (8-MOP) and 4,5'8-trimethylpsoralen (TMP) were compared with respect to their abilities to produce interstrand crosslinks in DNA. DNA from bacteriophage lambda, labeled with 32P, was subjected to sedimentation in alkaline sucrose gradients following exposure to several concentrations of 1 of the 2 psoralens and irradiation (UV-A, 360 nm) for various times. In alkaline sucrose gradients, crosslinked DNA molecules sediment about 1.4 times faster than undamaged DNA strands and the proportion of molecules carrying crosslinks can be estimated with reasonable accuracy. At equimolar psoralen concentrations (2 x 10(-7)M, or 4 x 10(-5)M) and with increasing irradiation times, the rate of production of crosslinked DNA was 4 to 30 times greater for TMP than for 8-MOP. Differences in the therqpeutic efficacy of 8-MOP and TMP in various clinical situations may be accounted for by the types of photoadducts formed by each drug as well as by their solubilities, rates of absorption and rates of metabolic degradation.", "contents": "Interstrand crosslinks in DNA of phage lambda after exposure to 8-methoxypsoralen and trimethylpsoralen in the presence of light. Two medically useful photosensitizing furocoumarins, 8-methoxypsoralen (8-MOP) and 4,5'8-trimethylpsoralen (TMP) were compared with respect to their abilities to produce interstrand crosslinks in DNA. DNA from bacteriophage lambda, labeled with 32P, was subjected to sedimentation in alkaline sucrose gradients following exposure to several concentrations of 1 of the 2 psoralens and irradiation (UV-A, 360 nm) for various times. In alkaline sucrose gradients, crosslinked DNA molecules sediment about 1.4 times faster than undamaged DNA strands and the proportion of molecules carrying crosslinks can be estimated with reasonable accuracy. At equimolar psoralen concentrations (2 x 10(-7)M, or 4 x 10(-5)M) and with increasing irradiation times, the rate of production of crosslinked DNA was 4 to 30 times greater for TMP than for 8-MOP. Differences in the therqpeutic efficacy of 8-MOP and TMP in various clinical situations may be accounted for by the types of photoadducts formed by each drug as well as by their solubilities, rates of absorption and rates of metabolic degradation."}
{"id": "PMID:469277", "title": "Cholinesterases isoenzymes: a comparative study in the skin and plasma.", "content": "Acetylcholinesterase (Ache) and pseudocholinesterase (BUche) activities were studied quantitatively in healthy skin by spectrophotometric methods and qualitatively by polyacrylamide gel electrophoresis. The results were compared to those obtained in plasma. The substrates used to reveal enzyme activities were acetylthiocholine (ATC) iodide and butyrylthiocholine (BTC) iodide, respectively. A linear relationship exists between the values of BUche and Ache activities in plasma and those in skin. Six isoenzymes of different electrophoretic mobility were observed in the skin. One of them, which is never found in plasma extracts, appears to be specific to the skin. On gradient gel electrophoresis, with both substrates (ATC and BTC), a single band of enzyme activity, corresponding to a molecular weight of 600,000 was observed. These results suggest that in the skin there is only one enzyme, most probably butyryl cholinesterase, which cleaves BTC somewhat faster than ATC. This methodology, when applied to the study of dermatoses in which abnormalities of cutaneous nerve terminals are suspected, should furnish precise functional pathophysiological details.", "contents": "Cholinesterases isoenzymes: a comparative study in the skin and plasma. Acetylcholinesterase (Ache) and pseudocholinesterase (BUche) activities were studied quantitatively in healthy skin by spectrophotometric methods and qualitatively by polyacrylamide gel electrophoresis. The results were compared to those obtained in plasma. The substrates used to reveal enzyme activities were acetylthiocholine (ATC) iodide and butyrylthiocholine (BTC) iodide, respectively. A linear relationship exists between the values of BUche and Ache activities in plasma and those in skin. Six isoenzymes of different electrophoretic mobility were observed in the skin. One of them, which is never found in plasma extracts, appears to be specific to the skin. On gradient gel electrophoresis, with both substrates (ATC and BTC), a single band of enzyme activity, corresponding to a molecular weight of 600,000 was observed. These results suggest that in the skin there is only one enzyme, most probably butyryl cholinesterase, which cleaves BTC somewhat faster than ATC. This methodology, when applied to the study of dermatoses in which abnormalities of cutaneous nerve terminals are suspected, should furnish precise functional pathophysiological details."}
{"id": "PMID:469278", "title": "Dermatological and ocular examinations in rabbits chronically photosensitized with methoxsalen.", "content": "Four groups of female Dutch-belted rabbits (Oryctulagus cuniculus) were given methoxsalen (12 mg/kg) or placebo by oral intubation and 1 hr later were exposed to UVA for either 2 or 8 hr. This procedure was repeated 5 days each week for 18 mo. A fifth group received no drug and no UVA exposure. The skin of the animals given methoxsalen and UVA showed signs of acute and chronic phototoxicity. Multiple peripheral blood parameters of hepatic, renal and hematologic function were normal and were not different between groups. Complete ophthalmoscopic examinations were performed periodically. No cataracts were seen in any of the animals. This data provides the perspective that in one species the daily dose of methoxsalen and UVA required to induce chronic cutaneous photosensitization is lower than the daily dose required to induce cataracts. It is inadvisable to interpret this data as suggesting that no risk exists for patients being treated with oral methoxsalen photochemotherapy. The experimental evidence supporting photosensitization as a cause of cataracts and implicating a role of lens DNA in this cataractogenesis is reviewed. Because methoxsalen-UVA alterations of lens DNA or protein could lead to delayed onset of cataracts, and because of the serious nature and potential preventability of phototoxic lens opacification, appropriate protective eye wear is recommended for all patients receiving oral psoralen photochemotherapy.", "contents": "Dermatological and ocular examinations in rabbits chronically photosensitized with methoxsalen. Four groups of female Dutch-belted rabbits (Oryctulagus cuniculus) were given methoxsalen (12 mg/kg) or placebo by oral intubation and 1 hr later were exposed to UVA for either 2 or 8 hr. This procedure was repeated 5 days each week for 18 mo. A fifth group received no drug and no UVA exposure. The skin of the animals given methoxsalen and UVA showed signs of acute and chronic phototoxicity. Multiple peripheral blood parameters of hepatic, renal and hematologic function were normal and were not different between groups. Complete ophthalmoscopic examinations were performed periodically. No cataracts were seen in any of the animals. This data provides the perspective that in one species the daily dose of methoxsalen and UVA required to induce chronic cutaneous photosensitization is lower than the daily dose required to induce cataracts. It is inadvisable to interpret this data as suggesting that no risk exists for patients being treated with oral methoxsalen photochemotherapy. The experimental evidence supporting photosensitization as a cause of cataracts and implicating a role of lens DNA in this cataractogenesis is reviewed. Because methoxsalen-UVA alterations of lens DNA or protein could lead to delayed onset of cataracts, and because of the serious nature and potential preventability of phototoxic lens opacification, appropriate protective eye wear is recommended for all patients receiving oral psoralen photochemotherapy."}
{"id": "PMID:469280", "title": "The influence of longwave ultraviolet radiation on sunburn cell production by UVB.", "content": "The potentiation of sunburn by longwave ultraviolet radiation (photoaugmentation) has been examined with regard to the influence of these waves on sunburn cell production in human skin. It was found that UVA did not enhance sunburn cell production. Photoaugmentation is limited to the erythemal component of the sunburn reaction.", "contents": "The influence of longwave ultraviolet radiation on sunburn cell production by UVB. The potentiation of sunburn by longwave ultraviolet radiation (photoaugmentation) has been examined with regard to the influence of these waves on sunburn cell production in human skin. It was found that UVA did not enhance sunburn cell production. Photoaugmentation is limited to the erythemal component of the sunburn reaction."}
{"id": "PMID:469366", "title": "Antibody method for measurement of dihydrotestosterone receptors in cultured human skin fibroblasts.", "content": "We have adapted the method of Casta\u00f1eda and Liao to the assay of DHT receptors in cultured fibroblasts arising from human skin. An antitestosterone antibody (100% crossreactivity for DHT) was coupled to CNBr-activated Sepharose. Confluent monolayers of fibroblasts were incubated with 3H-DHT (2 nM) at 37 degrees C for 30 min. Fibroblasts were then collected, sonicated, and centrifuged at 1200 x g for 15 min. The receptor assay was carried out on the supernatant; the antibody-sepharose was used to remove both unbound and nonspecifically bound DHT. Experience showed that the antibody did not entirely remove the nonspecifically bound and free DHT. A blank (sample heated at 60 degrees C for 3 min) was therefore subtracted to obtain an accurate value of specifically bound DHT. In spite of this, the antibody method, when compared to the gel filtration method, was more rapid and more convenient. Its reproducibility was similar to that of the gel filtration method, and its sensitivity was somewhat greater in patients with low levels of DHT-receptor complex. Improved sensitivity could be particularly useful when dealing with partial AIS.", "contents": "Antibody method for measurement of dihydrotestosterone receptors in cultured human skin fibroblasts. We have adapted the method of Casta\u00f1eda and Liao to the assay of DHT receptors in cultured fibroblasts arising from human skin. An antitestosterone antibody (100% crossreactivity for DHT) was coupled to CNBr-activated Sepharose. Confluent monolayers of fibroblasts were incubated with 3H-DHT (2 nM) at 37 degrees C for 30 min. Fibroblasts were then collected, sonicated, and centrifuged at 1200 x g for 15 min. The receptor assay was carried out on the supernatant; the antibody-sepharose was used to remove both unbound and nonspecifically bound DHT. Experience showed that the antibody did not entirely remove the nonspecifically bound and free DHT. A blank (sample heated at 60 degrees C for 3 min) was therefore subtracted to obtain an accurate value of specifically bound DHT. In spite of this, the antibody method, when compared to the gel filtration method, was more rapid and more convenient. Its reproducibility was similar to that of the gel filtration method, and its sensitivity was somewhat greater in patients with low levels of DHT-receptor complex. Improved sensitivity could be particularly useful when dealing with partial AIS."}
{"id": "PMID:469367", "title": "In vitro effects of single alkylating agents on normal peripheral blood lymphocytes.", "content": "The separate in vitro effects of HN2 and L-PAM on resting and stimulated peripheral blood lymphocytes were evaluated with biochemical and morphologic experimental endpoints. Both alkylating agents caused dose-dependent reduction of protein, RNA, and DNA synthesis, but the patterns of diminution differed. The number of cells staining with Erythrosin B, as a toxicity indicator, also rose with higher drug concentrations, but a large proportion of lymphocytes remained unstained even at the maximum drug dose. Stimulation with PHA partially nullified the suppression caused by HN2 but did not influence the effects of L-PAM. Exposure to PWM rendered the in vitro HN2 innocuous but L-PAM remained cytotoxic. Simultaneous lectin-induced blastogenesis proceeded unaltered. Finally, comparisons between 72 and 4 hr drug exposures imply that interference with intracellular synthesis occurs promptly, continues after drug removal, and is related quantitatively to drug concentration rather than to duration of contact.", "contents": "In vitro effects of single alkylating agents on normal peripheral blood lymphocytes. The separate in vitro effects of HN2 and L-PAM on resting and stimulated peripheral blood lymphocytes were evaluated with biochemical and morphologic experimental endpoints. Both alkylating agents caused dose-dependent reduction of protein, RNA, and DNA synthesis, but the patterns of diminution differed. The number of cells staining with Erythrosin B, as a toxicity indicator, also rose with higher drug concentrations, but a large proportion of lymphocytes remained unstained even at the maximum drug dose. Stimulation with PHA partially nullified the suppression caused by HN2 but did not influence the effects of L-PAM. Exposure to PWM rendered the in vitro HN2 innocuous but L-PAM remained cytotoxic. Simultaneous lectin-induced blastogenesis proceeded unaltered. Finally, comparisons between 72 and 4 hr drug exposures imply that interference with intracellular synthesis occurs promptly, continues after drug removal, and is related quantitatively to drug concentration rather than to duration of contact."}
{"id": "PMID:469370", "title": "The measurement of erythrocyte deformability using micropore membranes. A sensitive technique with clinical applications.", "content": "This article describes a positive-pressure filtration technique using low pore-density, 3 microns pore-diameter polycarbonate membranes employed to evaluate erythrocyte deformability in a clinically oriented hematology laboratory. Mean erythrocyte resistance to filtration was expressed by a numerical index which takes into account both the initial resistance of dilute red cell suspensions to passage across the membrane and the relative pressure rise observed after filtration of 30 ml of the same suspension (3 . 10(8) cells). The resistance index of 99 blood samples obtained from 88 healthy adult volunteers ranged between 1.4 and 2.9, with normal Gaussian distribution and mean of 2.04. Values obtained on similarly prepared samples from 20 patients with various hemolytic anemias always fell outside this range, indicating a reduced deformability in every case. The existence of a strong positive correlation was found between the resistance index and the degree of reticulocytosis in these patients. This method appears more sensitive than previously described filtration techniques in detecting the presence of small numbers of poorly deformable erythrocytes in vivo, while being more practical and statistically more significant than the micropipette elastimetry technique. Our results, however, raise a new question concerning the role of reticulocytes in the evaluation of red cell deformability on blood samples from patients with hemolytic anemia.", "contents": "The measurement of erythrocyte deformability using micropore membranes. A sensitive technique with clinical applications. This article describes a positive-pressure filtration technique using low pore-density, 3 microns pore-diameter polycarbonate membranes employed to evaluate erythrocyte deformability in a clinically oriented hematology laboratory. Mean erythrocyte resistance to filtration was expressed by a numerical index which takes into account both the initial resistance of dilute red cell suspensions to passage across the membrane and the relative pressure rise observed after filtration of 30 ml of the same suspension (3 . 10(8) cells). The resistance index of 99 blood samples obtained from 88 healthy adult volunteers ranged between 1.4 and 2.9, with normal Gaussian distribution and mean of 2.04. Values obtained on similarly prepared samples from 20 patients with various hemolytic anemias always fell outside this range, indicating a reduced deformability in every case. The existence of a strong positive correlation was found between the resistance index and the degree of reticulocytosis in these patients. This method appears more sensitive than previously described filtration techniques in detecting the presence of small numbers of poorly deformable erythrocytes in vivo, while being more practical and statistically more significant than the micropipette elastimetry technique. Our results, however, raise a new question concerning the role of reticulocytes in the evaluation of red cell deformability on blood samples from patients with hemolytic anemia."}
{"id": "PMID:469371", "title": "Alterations of intestinal and renal functions in rats after intraperitoneal injections of lead acetate.", "content": "When lead acetate was administered intraperitoneally to young rats at a dose of 20 mg/kg (five times a week for 6 weeks), their growth rate was retarded when compared with controls injected with sodium acetate. Only a small amount of the heavy metal reached the circulation and exerted limited effects on typical target organs. However, large, electron-dense inclusion bodies were found in the abdominal cavity. The in vivo intestinal absorption of glucose was reduced. When perfused at 40 mM concentration, the experimental animals had a mean absorption rate of 152.1 nmol/min . cm vs. 230.6 in the controls (p less than 0.01). Also, sodium and potassium transport was reduced. No effects were observed on amino acid transport and (Na+-K+)-ATPase. Mg++-ATPase, glucose-6-phosphatase, fructose-1, 6-diphosphatase, pyruvate kinase, succinic dehydrogenase, and tryptophan hydroxylase in the small intestinal mucosa and the kidney were unaltered. Renal alkaline phosphatase was decreased. These studies confirm the greater susceptibility of some active transport mechanisms of the small intestinal mucosa to lead toxicity, compared to those of the kidney.", "contents": "Alterations of intestinal and renal functions in rats after intraperitoneal injections of lead acetate. When lead acetate was administered intraperitoneally to young rats at a dose of 20 mg/kg (five times a week for 6 weeks), their growth rate was retarded when compared with controls injected with sodium acetate. Only a small amount of the heavy metal reached the circulation and exerted limited effects on typical target organs. However, large, electron-dense inclusion bodies were found in the abdominal cavity. The in vivo intestinal absorption of glucose was reduced. When perfused at 40 mM concentration, the experimental animals had a mean absorption rate of 152.1 nmol/min . cm vs. 230.6 in the controls (p less than 0.01). Also, sodium and potassium transport was reduced. No effects were observed on amino acid transport and (Na+-K+)-ATPase. Mg++-ATPase, glucose-6-phosphatase, fructose-1, 6-diphosphatase, pyruvate kinase, succinic dehydrogenase, and tryptophan hydroxylase in the small intestinal mucosa and the kidney were unaltered. Renal alkaline phosphatase was decreased. These studies confirm the greater susceptibility of some active transport mechanisms of the small intestinal mucosa to lead toxicity, compared to those of the kidney."}
{"id": "PMID:469372", "title": "Reversal of skeletal resistance to parathyroid hormone in uremia by vitamin D metabolites: evidence for the requirement of 1,25(OH)2D3 and 24,25(OH)2D3.", "content": "This study evaluates the role of vitamin D metabolites in the genesis of the skeletal resistance to the calcemic action of PTH in uremia. The changes in serum calcium after infusion of 2 U of PTE per kilogram per hour for 8 hr were evaluated in thyroparathyroidectomized dogs before and after 1 and 3 days of acute uremia produced by bilateral nephrectomy. The animals received vitamin D metabolites during the 3 days of uremia. Supplementation of 0.68 microgram/day 1,25(OH)2D3 and 24R,25(OH)2D3 restored the calcemic response to PTE to normal. This is in contrast to only partial correction of the response to PTE by 1,25(OH)2D3 alone. Administration of 1.36 microgram/day 24R,25(OH)2D3 did not improve the calcemic response to PTE. The results indicate that (1) both 1,25(OH)2D3 and 24R,25(OH)2D3 are necessary for the complete reversal of the impaired calcemic response to PTE, (2) this effect is not due to the increase in the amount of the dihydroxylated compounds of vitamin D, since equivalent amounts of these compounds in the form of 24R,25(OH)2D3 alone had no effect, and (3) the better effect of the combination of 1,25(OH)2D3 and 24R,25(OH)2D3 is most probably due to an interaction between these two metabolites of vitamin D permitting an intact calcemic action of PTH.", "contents": "Reversal of skeletal resistance to parathyroid hormone in uremia by vitamin D metabolites: evidence for the requirement of 1,25(OH)2D3 and 24,25(OH)2D3. This study evaluates the role of vitamin D metabolites in the genesis of the skeletal resistance to the calcemic action of PTH in uremia. The changes in serum calcium after infusion of 2 U of PTE per kilogram per hour for 8 hr were evaluated in thyroparathyroidectomized dogs before and after 1 and 3 days of acute uremia produced by bilateral nephrectomy. The animals received vitamin D metabolites during the 3 days of uremia. Supplementation of 0.68 microgram/day 1,25(OH)2D3 and 24R,25(OH)2D3 restored the calcemic response to PTE to normal. This is in contrast to only partial correction of the response to PTE by 1,25(OH)2D3 alone. Administration of 1.36 microgram/day 24R,25(OH)2D3 did not improve the calcemic response to PTE. The results indicate that (1) both 1,25(OH)2D3 and 24R,25(OH)2D3 are necessary for the complete reversal of the impaired calcemic response to PTE, (2) this effect is not due to the increase in the amount of the dihydroxylated compounds of vitamin D, since equivalent amounts of these compounds in the form of 24R,25(OH)2D3 alone had no effect, and (3) the better effect of the combination of 1,25(OH)2D3 and 24R,25(OH)2D3 is most probably due to an interaction between these two metabolites of vitamin D permitting an intact calcemic action of PTH."}
{"id": "PMID:469373", "title": "Phenolsulphotransferase: enzyme activity and endogenous inhibitors in the human erythrocyte.", "content": "PST (E.C. 2.8.2.1) plays an important role in the metabolism of many drugs, catecholamine metabolites, and catecholamines. PST activity was detected in each of 178 human RBC lysates. When MHPG was used as a substrate, the activity ranged from 28 to 1385 U/ml of RBC, with an average value of 217.7 +/- 13.1 (mean +/- S.E.M.). However, there was not a direct relationship between quantity of RBC lysate and enzyme activity, an observation that raised the possibility of endogenous enzyme inhibitors. Therefore human RBC PST was partially purified (415-fold) to use in the study of tissue enzyme inhibitors. The pH optimum of the partially purified enzyme was 7.5, with an apparent Km value for [35S]PAPS of 0.46 microM and an apparent Km value for MHPG of 260 microM. When the partially purified enzyme was added to each of 20 human RBC lysates, its activity was inhibited an average of 91% +/- 0.6 (mean +/- S.E.M.). Endogenous inhibitors were also present in homogenates of human renal cortex and in homogenates of a variety of rat tissues. RBC lysates contained at least two PST inhibitors: a low-molecular-weight inhibitor (less than 2000) that was heat-, acid-, and base-stable, dialyzable, and resistant to digestion by chymotrypsin; and a high-molecular-weight inhibitor (greater than 65,000) that was heat-labile and nondialyzable. Whether the RBC enzyme activity may serve as an indicator of PST activity in less accessible tissues remains to be determined. However, the first step toward testing this hypothesis will require the accurate measurement of PST activity in tissue preparations by a procedure that removes or inactivates enzyme inhibitors.", "contents": "Phenolsulphotransferase: enzyme activity and endogenous inhibitors in the human erythrocyte. PST (E.C. 2.8.2.1) plays an important role in the metabolism of many drugs, catecholamine metabolites, and catecholamines. PST activity was detected in each of 178 human RBC lysates. When MHPG was used as a substrate, the activity ranged from 28 to 1385 U/ml of RBC, with an average value of 217.7 +/- 13.1 (mean +/- S.E.M.). However, there was not a direct relationship between quantity of RBC lysate and enzyme activity, an observation that raised the possibility of endogenous enzyme inhibitors. Therefore human RBC PST was partially purified (415-fold) to use in the study of tissue enzyme inhibitors. The pH optimum of the partially purified enzyme was 7.5, with an apparent Km value for [35S]PAPS of 0.46 microM and an apparent Km value for MHPG of 260 microM. When the partially purified enzyme was added to each of 20 human RBC lysates, its activity was inhibited an average of 91% +/- 0.6 (mean +/- S.E.M.). Endogenous inhibitors were also present in homogenates of human renal cortex and in homogenates of a variety of rat tissues. RBC lysates contained at least two PST inhibitors: a low-molecular-weight inhibitor (less than 2000) that was heat-, acid-, and base-stable, dialyzable, and resistant to digestion by chymotrypsin; and a high-molecular-weight inhibitor (greater than 65,000) that was heat-labile and nondialyzable. Whether the RBC enzyme activity may serve as an indicator of PST activity in less accessible tissues remains to be determined. However, the first step toward testing this hypothesis will require the accurate measurement of PST activity in tissue preparations by a procedure that removes or inactivates enzyme inhibitors."}
{"id": "PMID:469374", "title": "A direct radioimmunoassay of rat urinary kallikrein and comparison with other measures of urinary kallikrein activity.", "content": "A highly sensitive radioimmunoassay for rat urinary kallikrein (minimal detectable amount, 40 pg/tube) has been developed. The assay uses a sheep antibody (Keq = 3.25 X 10(10)M-1) against purified Sprague-Dawley rat urinary kallikrein in a final dilution of 1 : 1,200,000. The assay incorporates a convenient and inexpensive PEG technique for separation of free from bound antigen. Parallel standard curves with rat urine or kidney homogenates were obtained. No cross-reactivity with human or dog urine samples or purified human urinary kallikrein was seen. Correlations among this assay, an esterolytic method, and a kininogenase radioimmunoassay for kallikrein were highly significant, with only the esterolytic assay demonstrating any significant nonspecificity. The radioimmunoassay can detect changes in urinary kallikrein levels produced by a maneuver known to alter urinary kallikrein excretion.", "contents": "A direct radioimmunoassay of rat urinary kallikrein and comparison with other measures of urinary kallikrein activity. A highly sensitive radioimmunoassay for rat urinary kallikrein (minimal detectable amount, 40 pg/tube) has been developed. The assay uses a sheep antibody (Keq = 3.25 X 10(10)M-1) against purified Sprague-Dawley rat urinary kallikrein in a final dilution of 1 : 1,200,000. The assay incorporates a convenient and inexpensive PEG technique for separation of free from bound antigen. Parallel standard curves with rat urine or kidney homogenates were obtained. No cross-reactivity with human or dog urine samples or purified human urinary kallikrein was seen. Correlations among this assay, an esterolytic method, and a kininogenase radioimmunoassay for kallikrein were highly significant, with only the esterolytic assay demonstrating any significant nonspecificity. The radioimmunoassay can detect changes in urinary kallikrein levels produced by a maneuver known to alter urinary kallikrein excretion."}
{"id": "PMID:469375", "title": "Postfeeding radioimmunoassay artefact in heparinized pig plasma: studies with intestinal calcium-binding protein.", "content": "Samples of pig blood were taken in relation to feeding and assayed for intestinal CaBP by radioimmunoassay. When heparinized plasma was assayed by a double-antibody technique, significant postprandial increases of plasma immunoreactivity between twofold and 10-fold were noted. These apparent feeding responses were seen only with heparinized plasma and not with EDTA plasma, ACD plasma, or serum. Nor were such responses seen when heparinized plasma was assayed with talc or charcoal methods for phase seperation. The height of the artefactual response in immunoreactivity following feeding was related to the amount of carrier serum added during the incubation with second antibody and with the duration of this incubation. The artefact was seen in the presence of 0.01M EDTA. These results demonstrate a striking postfeeding radioimmunoassay artefact localized to the second-antibody precipitation step. The artefact can easily be avoided by several methodological changes. Such an artefact could conceivably affect other double-antibody radioimmunoassays of hormones or other substances. Caution is advised when double-antibody radioimmunoassays are used to assay samples taken in relation to feeding.", "contents": "Postfeeding radioimmunoassay artefact in heparinized pig plasma: studies with intestinal calcium-binding protein. Samples of pig blood were taken in relation to feeding and assayed for intestinal CaBP by radioimmunoassay. When heparinized plasma was assayed by a double-antibody technique, significant postprandial increases of plasma immunoreactivity between twofold and 10-fold were noted. These apparent feeding responses were seen only with heparinized plasma and not with EDTA plasma, ACD plasma, or serum. Nor were such responses seen when heparinized plasma was assayed with talc or charcoal methods for phase seperation. The height of the artefactual response in immunoreactivity following feeding was related to the amount of carrier serum added during the incubation with second antibody and with the duration of this incubation. The artefact was seen in the presence of 0.01M EDTA. These results demonstrate a striking postfeeding radioimmunoassay artefact localized to the second-antibody precipitation step. The artefact can easily be avoided by several methodological changes. Such an artefact could conceivably affect other double-antibody radioimmunoassays of hormones or other substances. Caution is advised when double-antibody radioimmunoassays are used to assay samples taken in relation to feeding."}
{"id": "PMID:469376", "title": "Abnormalities of hepatic mitochondrial urea-cycle enzyme activities and hepatic ultrastructure in acute fatty liver of pregnancy.", "content": "Two patients presenting with acute fatty liver of pregnancy were studied. Because of similarities between acute fatty liver of pregnancy and Reye's syndrome, we investigated hepatic ultrastructure, urea-cycle enzyme activities, and plasma amino acids. Initial liver biopsies obtained 12 and 21 days after the onset of illness demonstrated microvesicular fat deposition and mitochondrial ultrastructural changes, including pleomorphism and abundant crystalline inclusions. In both biopsies, activity of the mitochondrial urea-cycle enzyme OTC was markedly below normal limits. Activity of the other mitochondrial urea-cycle enzyme, CPS, was low in one patient. Abnormalities of these enzymes persisted in second biopsies obtained at 9 and 28 weeks, respectively. By 44 weeks all urea-cycle enzyme activities had returned to normal in one patient. However, in the other patient OTC activity was still reduced at 52 weeks, although it had doubled in comparison to previous biopsies. Morphological changes of the mitochondria generally improved in parallel with the urea-cycle enzymes. Plasma amino acids, obtained at the time of the initial biopsies, demonstrated a generalized hypoaminoacidemia with the exception of glutamate. Serial observations in patients with this rare disease indicate that there are similarities with Reye's syndrome, in particular, reduced activity of the mitochondrial urea-cycle enzymes. But there are important differences. (1) Enzymatic and ultrastructural abnormalities of mitochondria persist for a longer period of time than in Reye's syndrome. (2) Mitochondrial ultrastructure is different. (3) Plasma amino acid profiles are different.", "contents": "Abnormalities of hepatic mitochondrial urea-cycle enzyme activities and hepatic ultrastructure in acute fatty liver of pregnancy. Two patients presenting with acute fatty liver of pregnancy were studied. Because of similarities between acute fatty liver of pregnancy and Reye's syndrome, we investigated hepatic ultrastructure, urea-cycle enzyme activities, and plasma amino acids. Initial liver biopsies obtained 12 and 21 days after the onset of illness demonstrated microvesicular fat deposition and mitochondrial ultrastructural changes, including pleomorphism and abundant crystalline inclusions. In both biopsies, activity of the mitochondrial urea-cycle enzyme OTC was markedly below normal limits. Activity of the other mitochondrial urea-cycle enzyme, CPS, was low in one patient. Abnormalities of these enzymes persisted in second biopsies obtained at 9 and 28 weeks, respectively. By 44 weeks all urea-cycle enzyme activities had returned to normal in one patient. However, in the other patient OTC activity was still reduced at 52 weeks, although it had doubled in comparison to previous biopsies. Morphological changes of the mitochondria generally improved in parallel with the urea-cycle enzymes. Plasma amino acids, obtained at the time of the initial biopsies, demonstrated a generalized hypoaminoacidemia with the exception of glutamate. Serial observations in patients with this rare disease indicate that there are similarities with Reye's syndrome, in particular, reduced activity of the mitochondrial urea-cycle enzymes. But there are important differences. (1) Enzymatic and ultrastructural abnormalities of mitochondria persist for a longer period of time than in Reye's syndrome. (2) Mitochondrial ultrastructure is different. (3) Plasma amino acid profiles are different."}
{"id": "PMID:469378", "title": "Metabolic fate of exogenous delta-aminolevulinic acid in Dubin-Johnson syndrome.", "content": "The underlying mechanism of abnormal urinary distribution of CP isomers in DJS is unknown. We administered ALA to DJS patients and carriers as well as to normal controls, and urinary and biliary porphyrins and plasma bilirubin were studied. In sharp contrast to the remarkable increase in urinary CP-III excretion in normal controls (2234 nmol/gm creatinine during the first 2 hr) after ALA, very small increase was observed in DJS patients (19 nmol). In DJS carriers the increase took intermediate values (1122 nmol). The increments in urinary CP-I were smallest in DJS patients, and the peak of its increase was delayed. These data are compatible with the hypothesis that a carrier-mediated mechanism in transporting CP-gen isomers I and III from the liver cells to the plasma may be anomalous in DJS hepatocytes and that the increased urinary CP-I characteristic of DJS may be the result of a disturbance in the uptake process of CP-gen I, derived mainly from the erythropoietic tissues, by DJS liver cells. Although the biliary percent of CP-I in controls decreased after ALA, it remained unchanged in DJS, suggesting the existence of a bile canalicular barrier against CP-gen-III in DJS. The elevated biliary porphyrins and plasma bilirubin after ALA in DJS patients favor an idea that there may be no major enzymatic derangements in the metabolic sequences from ALA to bilirubin. The increased excretion of PP in DJS bile after ALA remained to be explained.", "contents": "Metabolic fate of exogenous delta-aminolevulinic acid in Dubin-Johnson syndrome. The underlying mechanism of abnormal urinary distribution of CP isomers in DJS is unknown. We administered ALA to DJS patients and carriers as well as to normal controls, and urinary and biliary porphyrins and plasma bilirubin were studied. In sharp contrast to the remarkable increase in urinary CP-III excretion in normal controls (2234 nmol/gm creatinine during the first 2 hr) after ALA, very small increase was observed in DJS patients (19 nmol). In DJS carriers the increase took intermediate values (1122 nmol). The increments in urinary CP-I were smallest in DJS patients, and the peak of its increase was delayed. These data are compatible with the hypothesis that a carrier-mediated mechanism in transporting CP-gen isomers I and III from the liver cells to the plasma may be anomalous in DJS hepatocytes and that the increased urinary CP-I characteristic of DJS may be the result of a disturbance in the uptake process of CP-gen I, derived mainly from the erythropoietic tissues, by DJS liver cells. Although the biliary percent of CP-I in controls decreased after ALA, it remained unchanged in DJS, suggesting the existence of a bile canalicular barrier against CP-gen-III in DJS. The elevated biliary porphyrins and plasma bilirubin after ALA in DJS patients favor an idea that there may be no major enzymatic derangements in the metabolic sequences from ALA to bilirubin. The increased excretion of PP in DJS bile after ALA remained to be explained."}
{"id": "PMID:469379", "title": "Vascular compartmentalization of plasma norepinephrine in normal man: the relationships between venous and arterial norepinephrine concentration and the urinary excretion of norepinephrine.", "content": "To examine whether the concentration of NE in human plasma is dependent on the vascular source of the sample and to examine the contribution of the kidney to urinary NE, 14 normal men were studied. Plasma samples were obtained from a superficial forearm vein, and radial artery and urine samples were obtained during 1 hr of recumbency and 1 hr of upright posture. The Vne was greater than Ane during both recumbency and upright posture in black males. Such differences were not seen in age-matched white subjects. Stimulation of the sympathetic nervous system by upright posture increased both Vne and Ane in all subjects. NE concentrations in simultaneously obtained arterial and venous samples were different during the time of cardiovascular adjustments to upright posture. The urinary Xne increased after standing. Endogenous CCr decreased, whereas apparent NE clearance, calculated from the Ane, increased after standing, suggesting that a major portion of the augmented urinary Xne induced by upright posture was from an intrarenal source. We conclude that the concentration of NE in human blood is related to the specific vascular bed from which the sample is obtained, and that urinary NE is not solely derived from plasma by glomerular filtration but also arises from an unidentified renal source.", "contents": "Vascular compartmentalization of plasma norepinephrine in normal man: the relationships between venous and arterial norepinephrine concentration and the urinary excretion of norepinephrine. To examine whether the concentration of NE in human plasma is dependent on the vascular source of the sample and to examine the contribution of the kidney to urinary NE, 14 normal men were studied. Plasma samples were obtained from a superficial forearm vein, and radial artery and urine samples were obtained during 1 hr of recumbency and 1 hr of upright posture. The Vne was greater than Ane during both recumbency and upright posture in black males. Such differences were not seen in age-matched white subjects. Stimulation of the sympathetic nervous system by upright posture increased both Vne and Ane in all subjects. NE concentrations in simultaneously obtained arterial and venous samples were different during the time of cardiovascular adjustments to upright posture. The urinary Xne increased after standing. Endogenous CCr decreased, whereas apparent NE clearance, calculated from the Ane, increased after standing, suggesting that a major portion of the augmented urinary Xne induced by upright posture was from an intrarenal source. We conclude that the concentration of NE in human blood is related to the specific vascular bed from which the sample is obtained, and that urinary NE is not solely derived from plasma by glomerular filtration but also arises from an unidentified renal source."}
{"id": "PMID:469380", "title": "Specific and quantitative method for estimation of platelet adhesion to fibrillar collagen.", "content": "A quantitative method (Sepharose test) was devised to measure the adhesion of blood platelets to fibrillar collagen. [14C]5HT-labeled platelets were isolated from plasma, resuspended in EDTA buffer, and incubated with buffer (control) or with fibrillar collagen for 150 sec at 33 degrees C. The mixtures were then filtered through Sepharose 2B columns. In controls the platelets were rapidly eluted, and this was confirmed after 51Cr labeling. [C]5HT was recovered in two stages: 60% with the platelets and 40% retarded, as free 5HT. After incubation with fibrillar collagen (50 micrograms), platelets were retained with the fibrils on the top of the column, and only free [14C]5HT (released from the platelets) was eluted. The percentage of adhesion depended on the number of platelets, the amount of collagen, its degree of polymerization, and the time of incubation at 33 degrees C. [14C]5HT release was markedly diminished when both incubation and filtration were performed at low temperature. ASA, used either in vitro or in vivo in rabbits, did not change the percentage of adhesion but significantly diminished the total amount of [14C]5HT eluted. This method offers a quantitative and reproducible system for the differentiation of adhesion and release, independent of platelet aggregation.", "contents": "Specific and quantitative method for estimation of platelet adhesion to fibrillar collagen. A quantitative method (Sepharose test) was devised to measure the adhesion of blood platelets to fibrillar collagen. [14C]5HT-labeled platelets were isolated from plasma, resuspended in EDTA buffer, and incubated with buffer (control) or with fibrillar collagen for 150 sec at 33 degrees C. The mixtures were then filtered through Sepharose 2B columns. In controls the platelets were rapidly eluted, and this was confirmed after 51Cr labeling. [C]5HT was recovered in two stages: 60% with the platelets and 40% retarded, as free 5HT. After incubation with fibrillar collagen (50 micrograms), platelets were retained with the fibrils on the top of the column, and only free [14C]5HT (released from the platelets) was eluted. The percentage of adhesion depended on the number of platelets, the amount of collagen, its degree of polymerization, and the time of incubation at 33 degrees C. [14C]5HT release was markedly diminished when both incubation and filtration were performed at low temperature. ASA, used either in vitro or in vivo in rabbits, did not change the percentage of adhesion but significantly diminished the total amount of [14C]5HT eluted. This method offers a quantitative and reproducible system for the differentiation of adhesion and release, independent of platelet aggregation."}
{"id": "PMID:469381", "title": "Class- and subclass-specific antibody response to hemocyanin in nonmalignant paraproteinemia.", "content": "IgM, IgG, and IgA class-specific, as well as IgG subclass-specific antibody titers against the primary immunogen HPH were measured with ELISA in 19 patients with nonmalignant paraproteinemia (eight with IgG1, two with IgG2, two with IgG4, four with IgM, and three with IgA) and in a simultaneously studied age- and sex-matched control group. After primary immunization only IgM and IgA anti-HPH titers were significantly lower in the patient group. Four patients with relatively high IgG or IgA serum paraprotein levels did not produce antibodies in some Ig classes or IgG subclasses, whereas all other patients and all controls developed antibody titers in all classes and IgG subclasses. Low or absent antibody titers did not occur preferentially in the Ig (sub)classes to which the paraproteins belonged. After secondary immunization the patients could not increase or maintain their antibody titers as well as the controls, and this was most clear in the IgM and IgA antibody class. A direct correlation between polyclonal serum IgM levels and IgM anti-HPH titers was present in the patients. Such a correlation was absent for IgA in the patients and for all classes in the controls. It is concluded that humoral immunosuppression as measured with a newly encountered antigen in patients with nonmalignant paraproteinemia is most clearly expressed in the IgM and IgA antibody class and that the paraprotein (sub)class is not preferentially involved.", "contents": "Class- and subclass-specific antibody response to hemocyanin in nonmalignant paraproteinemia. IgM, IgG, and IgA class-specific, as well as IgG subclass-specific antibody titers against the primary immunogen HPH were measured with ELISA in 19 patients with nonmalignant paraproteinemia (eight with IgG1, two with IgG2, two with IgG4, four with IgM, and three with IgA) and in a simultaneously studied age- and sex-matched control group. After primary immunization only IgM and IgA anti-HPH titers were significantly lower in the patient group. Four patients with relatively high IgG or IgA serum paraprotein levels did not produce antibodies in some Ig classes or IgG subclasses, whereas all other patients and all controls developed antibody titers in all classes and IgG subclasses. Low or absent antibody titers did not occur preferentially in the Ig (sub)classes to which the paraproteins belonged. After secondary immunization the patients could not increase or maintain their antibody titers as well as the controls, and this was most clear in the IgM and IgA antibody class. A direct correlation between polyclonal serum IgM levels and IgM anti-HPH titers was present in the patients. Such a correlation was absent for IgA in the patients and for all classes in the controls. It is concluded that humoral immunosuppression as measured with a newly encountered antigen in patients with nonmalignant paraproteinemia is most clearly expressed in the IgM and IgA antibody class and that the paraprotein (sub)class is not preferentially involved."}
{"id": "PMID:469382", "title": "The antithrombotic effects of warfarin and heparin following infusions of tissue thromboplastin in rabbits: clinical implications.", "content": "An animal model for the production of stasis thrombi was employed to obtain the data reported in this study. Rabbits treated with warfarin (1.5 mg/kg/day) exhibited a maximal increase in prothrombin time and decreases in factor VII, factor X, and prothrombin within 48 hr with no additional changes occurring after 10 days of drug administration. In contrast, Xa inhibitory activity was unchanged after 48 hr of warfarin treatment but was significantly increased by the tenth day. When thrombosis was induced by infusions of 60 micrograms of tissue thromboplastin, the warfarin regimen produced an antithrombotic effect by the sixth hour, which increased to significance by day 2 and was further significantly increased by day 10. These three stages correspond to the initial depletion of the vitamin K-dependent clotting factors, the maximal depletion of these proteins, and the maximal increase in Xa inhibitory activity, respectively. Thus these experiments separate the antithrombotic potential of warfarin into two components: an early effect related to the decrease in factor VII and a delayed augmentation of Xa inhibitory acticity. Intravenous heparin alone (5 U/kg) did not protect against infusions of 60 micrograms of tissue thromboplastin but did provide an antithrombotic effect against 45 micrograms of the same infusate. Higher doses of heparin, however, did protect against infusion of 60 micrograms of tissue thromboplastin. After 48 hr of warfarin treatment, 5 U/kg heparin increased protection against 60 micrograms of tissue thromboplastin to a degree equivalent to that provided after 10 days of warfarin therapy alone.", "contents": "The antithrombotic effects of warfarin and heparin following infusions of tissue thromboplastin in rabbits: clinical implications. An animal model for the production of stasis thrombi was employed to obtain the data reported in this study. Rabbits treated with warfarin (1.5 mg/kg/day) exhibited a maximal increase in prothrombin time and decreases in factor VII, factor X, and prothrombin within 48 hr with no additional changes occurring after 10 days of drug administration. In contrast, Xa inhibitory activity was unchanged after 48 hr of warfarin treatment but was significantly increased by the tenth day. When thrombosis was induced by infusions of 60 micrograms of tissue thromboplastin, the warfarin regimen produced an antithrombotic effect by the sixth hour, which increased to significance by day 2 and was further significantly increased by day 10. These three stages correspond to the initial depletion of the vitamin K-dependent clotting factors, the maximal depletion of these proteins, and the maximal increase in Xa inhibitory activity, respectively. Thus these experiments separate the antithrombotic potential of warfarin into two components: an early effect related to the decrease in factor VII and a delayed augmentation of Xa inhibitory acticity. Intravenous heparin alone (5 U/kg) did not protect against infusions of 60 micrograms of tissue thromboplastin but did provide an antithrombotic effect against 45 micrograms of the same infusate. Higher doses of heparin, however, did protect against infusion of 60 micrograms of tissue thromboplastin. After 48 hr of warfarin treatment, 5 U/kg heparin increased protection against 60 micrograms of tissue thromboplastin to a degree equivalent to that provided after 10 days of warfarin therapy alone."}
{"id": "PMID:469383", "title": "Effects of cigarette smoke on elastase secretion by murine macrophages.", "content": "Mice were chronically exposed to cigarette smoke for various time periods up to 4 weeks. As a consequence of the exposure, there was an increase in the number of alveolar macrophages obtained from the lungs of these mice. Light microscopic examination of cultured cells revealed increased numbers of highly pleomorphic cells filled with pigmented residues of cigarette smoke. These cells were more mitotically active, with a five fold increase in the number of alveolar colony-forming cells compared to the controls. When macrophages derived from mice exposed to cigarette smoke were cultured at high density in the absence of serum, they secreted significantly greater amounts of elastase than did the same number of control macrophages. At concentrations as low as 0.50 micrograms/ml, cycloheximide reversibly inhibited elastase secretion from both the control and experimental cultures. The effects of cigarette smoke inhalation on elastase secretion by alveolar macrophages do not appear to be a direct effect of cigarette smoke on these cells. Exposure of normal mouse macrophages in vitro to pulses of aqueous extracts of cigarette smoke, while significantly increasing secretion of elastase by peritoneal exudative macrophages, did not augment that of resident or exudative alveolar macrophages. These results suggest that the increased elastase secretion observed with the use of cultured macrophages derived from mice exposed to cigarette smoke is the result of either indirect activation of resident macrophages or the recruitment of a highly activated exudative population into the lungs of the exposed animals.", "contents": "Effects of cigarette smoke on elastase secretion by murine macrophages. Mice were chronically exposed to cigarette smoke for various time periods up to 4 weeks. As a consequence of the exposure, there was an increase in the number of alveolar macrophages obtained from the lungs of these mice. Light microscopic examination of cultured cells revealed increased numbers of highly pleomorphic cells filled with pigmented residues of cigarette smoke. These cells were more mitotically active, with a five fold increase in the number of alveolar colony-forming cells compared to the controls. When macrophages derived from mice exposed to cigarette smoke were cultured at high density in the absence of serum, they secreted significantly greater amounts of elastase than did the same number of control macrophages. At concentrations as low as 0.50 micrograms/ml, cycloheximide reversibly inhibited elastase secretion from both the control and experimental cultures. The effects of cigarette smoke inhalation on elastase secretion by alveolar macrophages do not appear to be a direct effect of cigarette smoke on these cells. Exposure of normal mouse macrophages in vitro to pulses of aqueous extracts of cigarette smoke, while significantly increasing secretion of elastase by peritoneal exudative macrophages, did not augment that of resident or exudative alveolar macrophages. These results suggest that the increased elastase secretion observed with the use of cultured macrophages derived from mice exposed to cigarette smoke is the result of either indirect activation of resident macrophages or the recruitment of a highly activated exudative population into the lungs of the exposed animals."}
{"id": "PMID:469384", "title": "Relationship between packed cell volume, platelets, and platelet survival in red blood cell-hypertransfused mice.", "content": "In this study we have measured platelet and megakaryocyte concentration, blood volume, and platelet survival of mice after RBC hypertransfusion to PCVs of 62% to 90%. The platelet concentration of mice with PCVs up to 75% was decreased by up to one half. At higher PCVs a more severe thrombocytopenia developed, with platelet concentrations decreased to less than 10% of baseline in approximately one half of the mice. Blood volumes of the hypertransfused mice were increased up to twofold. Megakaryocyte concentrations were normal or increased. Platelet survival in mice with PCVs less than 75% was normal but was sharply decreased for mice with higher PCVs. The decrease in platelet concentration at moderately elevated PCVs may be explained by hemodilution in the larger blood volume. However, hemodilution alone cannot explain the severe thrombocytopenia at higher PCVs. The presence of decreased platelet survival with normal or increased megakaryocyte concentrations in this latter group suggests that the severe thrombocytopenia is the result of more rapid platelet destruction. In summary, elevation of the PCV by RBC hypertransfusion produces thrombocytopenia. The severity of the thrombocytopenia and the mechanisms involved in producing it change abruptly when the PCV exceeds 75%. These findings should be considered in interpretations of the influence of RBC hypertransfusion on hematopoiesis and in clinical and experimental studies of thrombopoiesis in polycythemic subjects.", "contents": "Relationship between packed cell volume, platelets, and platelet survival in red blood cell-hypertransfused mice. In this study we have measured platelet and megakaryocyte concentration, blood volume, and platelet survival of mice after RBC hypertransfusion to PCVs of 62% to 90%. The platelet concentration of mice with PCVs up to 75% was decreased by up to one half. At higher PCVs a more severe thrombocytopenia developed, with platelet concentrations decreased to less than 10% of baseline in approximately one half of the mice. Blood volumes of the hypertransfused mice were increased up to twofold. Megakaryocyte concentrations were normal or increased. Platelet survival in mice with PCVs less than 75% was normal but was sharply decreased for mice with higher PCVs. The decrease in platelet concentration at moderately elevated PCVs may be explained by hemodilution in the larger blood volume. However, hemodilution alone cannot explain the severe thrombocytopenia at higher PCVs. The presence of decreased platelet survival with normal or increased megakaryocyte concentrations in this latter group suggests that the severe thrombocytopenia is the result of more rapid platelet destruction. In summary, elevation of the PCV by RBC hypertransfusion produces thrombocytopenia. The severity of the thrombocytopenia and the mechanisms involved in producing it change abruptly when the PCV exceeds 75%. These findings should be considered in interpretations of the influence of RBC hypertransfusion on hematopoiesis and in clinical and experimental studies of thrombopoiesis in polycythemic subjects."}
{"id": "PMID:469388", "title": "Metabolic investigations in Meni\u00e8re's disease. Preliminary findings.", "content": "The aetiology of Meni\u00e8re's disease is unknown but in recent years many theories have been advanced to explain the observed pathological changes. These include abnormal metabolic states, allergy and personality type. In an attempt to throw more light on this and to confirm or refute the earlier work we studied twenty-seven (fifteen male:twelve female) patients with Meni\u00e8re's disease who conformed to the criteria set out by the Committee of Hearing and Equilibration of the American Academy of Ophthalmology and Otolaryngology (Alford, 1972). In this series the incidence of abnormal metabolic states was no higher than that of a comparable group in the general population. There was no significant difference in response to pin prick skin tests compared with a control group. Klockhoff and Lindblom's (1966) glycerol dehydration test was applied to twenty-one patients. The response to this test was measured by changes in the osmolality of the serum, the pure tone threshold of hearing and the maximum speech discrimination score. Continuous transtympanic electrocochleographic recordings during glycerol dehydration were carried out and in 10 patients there was a definite decrease in the negative summating potential. An electrophysiological explanation for this has been put forward and the possible use of this measurement in the diagnosis and management of Meni\u00e8re's disease has been discussed. The intercorrelation between these measures and their predictive value will become apparent in the future.", "contents": "Metabolic investigations in Meni\u00e8re's disease. Preliminary findings. The aetiology of Meni\u00e8re's disease is unknown but in recent years many theories have been advanced to explain the observed pathological changes. These include abnormal metabolic states, allergy and personality type. In an attempt to throw more light on this and to confirm or refute the earlier work we studied twenty-seven (fifteen male:twelve female) patients with Meni\u00e8re's disease who conformed to the criteria set out by the Committee of Hearing and Equilibration of the American Academy of Ophthalmology and Otolaryngology (Alford, 1972). In this series the incidence of abnormal metabolic states was no higher than that of a comparable group in the general population. There was no significant difference in response to pin prick skin tests compared with a control group. Klockhoff and Lindblom's (1966) glycerol dehydration test was applied to twenty-one patients. The response to this test was measured by changes in the osmolality of the serum, the pure tone threshold of hearing and the maximum speech discrimination score. Continuous transtympanic electrocochleographic recordings during glycerol dehydration were carried out and in 10 patients there was a definite decrease in the negative summating potential. An electrophysiological explanation for this has been put forward and the possible use of this measurement in the diagnosis and management of Meni\u00e8re's disease has been discussed. The intercorrelation between these measures and their predictive value will become apparent in the future."}
{"id": "PMID:469389", "title": "Obliterative otitis media.", "content": "The clinical history, pathology and results of tympanoplasty in 12 patients with obliterative otitis are described. The condition is characterized by an intact, not retracted, thick drum and a middle ear totally filled with fibrous tissue and small cholesterol granulomas. The tube is anatomically obstructed in most cases. The results are poor and it is concluded that tympanoplasty is not indicated in this disease.", "contents": "Obliterative otitis media. The clinical history, pathology and results of tympanoplasty in 12 patients with obliterative otitis are described. The condition is characterized by an intact, not retracted, thick drum and a middle ear totally filled with fibrous tissue and small cholesterol granulomas. The tube is anatomically obstructed in most cases. The results are poor and it is concluded that tympanoplasty is not indicated in this disease."}
{"id": "PMID:469391", "title": "Amniotic graft in the management of severe epistaxis due to hereditary haemorrhagic telangiectasia.", "content": "In selected cases of Hereditary Haemorrhagic Telangiectesia with severe, life-threatening epistaxis, a new surgical procedure has been performed. It consists of a thorough, near total excision of the diseased nasal mucosa and its replacement by an amniotic memebrane graft. The surgical details dealing with preparation of the amniotic graft are described. Surgery has been performed by this technique in six cases. During 9 to 30 min months of follow-up, no nasal bleeding has occurred and the haemoglobin level has remained normal. The aminiotic membrane has served as an adequate grafting material for the lining of the nasal chambers.", "contents": "Amniotic graft in the management of severe epistaxis due to hereditary haemorrhagic telangiectasia. In selected cases of Hereditary Haemorrhagic Telangiectesia with severe, life-threatening epistaxis, a new surgical procedure has been performed. It consists of a thorough, near total excision of the diseased nasal mucosa and its replacement by an amniotic memebrane graft. The surgical details dealing with preparation of the amniotic graft are described. Surgery has been performed by this technique in six cases. During 9 to 30 min months of follow-up, no nasal bleeding has occurred and the haemoglobin level has remained normal. The aminiotic membrane has served as an adequate grafting material for the lining of the nasal chambers."}
{"id": "PMID:469393", "title": "Changes in the lower respiratory epithelia induced by oestrogen intake. An experimental histopathological study.", "content": "Fifty-eight male and non-pregnant female guinea pigs with an average weight of 400 gm were used in this work, ten animals as a control group, the remaineder receiving oestrogen in the form of ethanyl oestradiol in a dose of 10 microgram/animal/day. Histopathological studies were carried out after periods of one, two, three, four and eight weeks, and three, six and twelve months respectively. The respiratory mucosa, at the different levels mentioned, showed definite changes. The surface epithelium showed metaplastic changes with mitotic figures, until complete and definite stratifications were reached. The cilia decreased and then disappeared completely, while the mucus-secreting goblet cells showed an initial augmentation in number and increased activity, followed by a gradual decrease in their number, until total disappearance occurred at the stage of stratification in the surface epithelium.", "contents": "Changes in the lower respiratory epithelia induced by oestrogen intake. An experimental histopathological study. Fifty-eight male and non-pregnant female guinea pigs with an average weight of 400 gm were used in this work, ten animals as a control group, the remaineder receiving oestrogen in the form of ethanyl oestradiol in a dose of 10 microgram/animal/day. Histopathological studies were carried out after periods of one, two, three, four and eight weeks, and three, six and twelve months respectively. The respiratory mucosa, at the different levels mentioned, showed definite changes. The surface epithelium showed metaplastic changes with mitotic figures, until complete and definite stratifications were reached. The cilia decreased and then disappeared completely, while the mucus-secreting goblet cells showed an initial augmentation in number and increased activity, followed by a gradual decrease in their number, until total disappearance occurred at the stage of stratification in the surface epithelium."}
{"id": "PMID:469394", "title": "Pituitary calcospherites: an aid to highly selective hypophysectomy.", "content": "A case of a prolactin-secreting pituitary adenoma containing calcospherites is reported. The difficulties in achieving a highly selective microdissection of large pituitary tumours are discussed and the value of calcification within a prolactinoma emphasized.", "contents": "Pituitary calcospherites: an aid to highly selective hypophysectomy. A case of a prolactin-secreting pituitary adenoma containing calcospherites is reported. The difficulties in achieving a highly selective microdissection of large pituitary tumours are discussed and the value of calcification within a prolactinoma emphasized."}
{"id": "PMID:469396", "title": "Cervical ranula.", "content": "Ranulas are mucous extravasation cysts, and usually originate from the sublingual salivary gland. They may occasionally infiltrate the tissue planes of the neck and present as a cervical tumour. The literature is reviewed, and four personal cases of cervical ranula presented. Successful treatment may be achieved in the vast majority of cases by removal of the sublingual salivary gland.", "contents": "Cervical ranula. Ranulas are mucous extravasation cysts, and usually originate from the sublingual salivary gland. They may occasionally infiltrate the tissue planes of the neck and present as a cervical tumour. The literature is reviewed, and four personal cases of cervical ranula presented. Successful treatment may be achieved in the vast majority of cases by removal of the sublingual salivary gland."}
{"id": "PMID:469399", "title": "Bone mineral content in osteogenesis imperfecta tarda and in otosclerosis.", "content": "In 22 patients with osteogenesis imperfecta and in 63 patients with otosclerosis the bone mineral content in peripheral bones was determined by photon absorptiometry. The bone mineral content proved significantly reduced in patients with osteogenesis imperfecta as compared with normals and with patients with otosclerosis. In the latter patients the bone mineral content was normal. These findings support the assumption that stapedial fixation in otosclerosis and in osteogenesis imperfecta is of different aetiology.", "contents": "Bone mineral content in osteogenesis imperfecta tarda and in otosclerosis. In 22 patients with osteogenesis imperfecta and in 63 patients with otosclerosis the bone mineral content in peripheral bones was determined by photon absorptiometry. The bone mineral content proved significantly reduced in patients with osteogenesis imperfecta as compared with normals and with patients with otosclerosis. In the latter patients the bone mineral content was normal. These findings support the assumption that stapedial fixation in otosclerosis and in osteogenesis imperfecta is of different aetiology."}
{"id": "PMID:469400", "title": "Histopathological changes in experimental free thyroid transplants.", "content": "Free thyroid autotransplants into new receptor sites have been tried in 28 dogs, by two different techniques. Good but irregular histopathological survival of thyroid acini has been found after free autotransplantation of bisected and everted thyroid lobes into the external genitalia of dogs. The results have been very encouraging and the functional assessment of these implants is essential; such work is already in progress in our laboratory. Our findings and their significance have been discussed.", "contents": "Histopathological changes in experimental free thyroid transplants. Free thyroid autotransplants into new receptor sites have been tried in 28 dogs, by two different techniques. Good but irregular histopathological survival of thyroid acini has been found after free autotransplantation of bisected and everted thyroid lobes into the external genitalia of dogs. The results have been very encouraging and the functional assessment of these implants is essential; such work is already in progress in our laboratory. Our findings and their significance have been discussed."}
{"id": "PMID:469401", "title": "Malignant oncocytoma of the nasal cavity.", "content": "Malignant oncocytoma of the major salivary glands is uncommon. Those of the endocrine, minor salivary and mucous glands are certainly rare. A case of a slowly-growing, low-grade malignant oncocytoma of the nasal cavity in a 50-year-old male, with no lymph node or distant metastases or local invasion of vital adjacent structures, and treated with combined radiotherapy and radical surgery, has been presented. The literature has been briefly reviewed, and the histological types; the electro-microscopic, histochemical and clinical pictures; and the outlines of treatment of oncocytomas have been discussed.", "contents": "Malignant oncocytoma of the nasal cavity. Malignant oncocytoma of the major salivary glands is uncommon. Those of the endocrine, minor salivary and mucous glands are certainly rare. A case of a slowly-growing, low-grade malignant oncocytoma of the nasal cavity in a 50-year-old male, with no lymph node or distant metastases or local invasion of vital adjacent structures, and treated with combined radiotherapy and radical surgery, has been presented. The literature has been briefly reviewed, and the histological types; the electro-microscopic, histochemical and clinical pictures; and the outlines of treatment of oncocytomas have been discussed."}
{"id": "PMID:469403", "title": "Parotid gland calculus. Report of a case.", "content": "A patient, was admitted with multiple calculi in the left parotid gland. Plain radiography and sialography confirmed the diagnosis. A partial parotidectomy was done and the calculi were removed.", "contents": "Parotid gland calculus. Report of a case. A patient, was admitted with multiple calculi in the left parotid gland. Plain radiography and sialography confirmed the diagnosis. A partial parotidectomy was done and the calculi were removed."}
{"id": "PMID:469404", "title": "A rare 'parotid tumour'.", "content": "A ganglion of the temporo-mandibular joint presenting as a tumour of the deep lobe of the parotid gland is described. This appears to be the first description of such a case in the literature. Simple excision of the lesion after mobilization of the superficial lobe was performed. This form of management gives a very good cosmetic result for the occasional simple cyst of the deep parotid lobe. The ganglion reported in this paper presented as a parotid tumour. It was managed by mobilization of the superficial part of the gland from the facial nerve until the nature of the lesion was determined. The fact that the superficial lobe of the gland was not excised has caused no ill effect to the patient but has given a better cosmetic result by preventing the usual pre-tragal depression. Conservative surgery has previously been suggested in similar cases (Brennan et al., 1970).", "contents": "A rare 'parotid tumour'. A ganglion of the temporo-mandibular joint presenting as a tumour of the deep lobe of the parotid gland is described. This appears to be the first description of such a case in the literature. Simple excision of the lesion after mobilization of the superficial lobe was performed. This form of management gives a very good cosmetic result for the occasional simple cyst of the deep parotid lobe. The ganglion reported in this paper presented as a parotid tumour. It was managed by mobilization of the superficial part of the gland from the facial nerve until the nature of the lesion was determined. The fact that the superficial lobe of the gland was not excised has caused no ill effect to the patient but has given a better cosmetic result by preventing the usual pre-tragal depression. Conservative surgery has previously been suggested in similar cases (Brennan et al., 1970)."}
{"id": "PMID:469411", "title": "Stochastic models for X chromosome inactivation.", "content": "A multivariate Gaussian model for mammalian development is presented with the associated biological and mathematical assumptions. Many biological investigations use the female mammal X chromosome to test hypotheses and to estimate parameters of the developmental system. In particular, Lyon's (1961) hypotheses are used as a basis of the mathematical model. Experimental mouse data and three sets of human experimental data are analyzed using the hypothesized Gaussian model. The estimated biological parameters are consistent with some current biological theories.", "contents": "Stochastic models for X chromosome inactivation. A multivariate Gaussian model for mammalian development is presented with the associated biological and mathematical assumptions. Many biological investigations use the female mammal X chromosome to test hypotheses and to estimate parameters of the developmental system. In particular, Lyon's (1961) hypotheses are used as a basis of the mathematical model. Experimental mouse data and three sets of human experimental data are analyzed using the hypothesized Gaussian model. The estimated biological parameters are consistent with some current biological theories."}
{"id": "PMID:469412", "title": "Spatial patterns for an interaction-diffusion equation in morphogenesis.", "content": "A certain interaction-diffusion equation occurring in morphogenesis is considered. This equation is proposed by Gierer and Meinhardt, which is introduced by Child's gradient theory and Turing's idea about diffusion driven instability. It is shown that slightly asymmetric gradients in the tissue produce stable striking patterns depending on its asymmetry, starting from uniform distribution of morphogens. The tool is the perturbed bifurcation theory. Moreover, from a mathematical point of view, the global existence of steady state solutions with respect to some parameters is discussed.", "contents": "Spatial patterns for an interaction-diffusion equation in morphogenesis. A certain interaction-diffusion equation occurring in morphogenesis is considered. This equation is proposed by Gierer and Meinhardt, which is introduced by Child's gradient theory and Turing's idea about diffusion driven instability. It is shown that slightly asymmetric gradients in the tissue produce stable striking patterns depending on its asymmetry, starting from uniform distribution of morphogens. The tool is the perturbed bifurcation theory. Moreover, from a mathematical point of view, the global existence of steady state solutions with respect to some parameters is discussed."}
{"id": "PMID:469413", "title": "A simple model for phase locking of biological oscillators.", "content": "A mathematical model is presented for phase locking of a biological oscillator to a sinusoidal stimulus. Analytical, numerical and topological considerations are used to discuss the patterns of phase locking as a function of amplitude of the sinusoidal stimulus and the relative frequencies of the osillator and the sinusoidal stimulus. The sorts of experimental data which are needed to make comparisons between theory and experiment are discussed.", "contents": "A simple model for phase locking of biological oscillators. A mathematical model is presented for phase locking of a biological oscillator to a sinusoidal stimulus. Analytical, numerical and topological considerations are used to discuss the patterns of phase locking as a function of amplitude of the sinusoidal stimulus and the relative frequencies of the osillator and the sinusoidal stimulus. The sorts of experimental data which are needed to make comparisons between theory and experiment are discussed."}
{"id": "PMID:469414", "title": "Representation of Nonepistatic selection models and analysis of multilocus Hardy-Weinberg Equilibrium configurations.", "content": "The paper develops conditions for the existence and the stability of central equilibria emanating from selection recombination interaction with generalized nonepistatic selection forms operating in multilocus multiallele systems. The selection structure admits a natural representation as simple sums of Kronecker products based on a common set of marginal selection components. A flexible parametrization of the recombination process is introduced leading to a canonical derivation of the transformation equations connecting gamete frequency states over successive generations. Conditions for the existence and stability of multilocus Hardy-Weinberg (H.W.) type equilibria are elaborated for the classical nonepistatic models (multiplicative and additive viability effects across loci) as well as for generalized nonepistatic selection expressions. It is established that the range of recombination distributions maintaining a stable H.W. polymorphic equilibrium is confined to loose linkage in the pure multiplicative case, but is not restricted in the additive model. In the bisexual case we ascertain for the generalized nonepistatic model the stability conditions of a common H.W polymorphism.", "contents": "Representation of Nonepistatic selection models and analysis of multilocus Hardy-Weinberg Equilibrium configurations. The paper develops conditions for the existence and the stability of central equilibria emanating from selection recombination interaction with generalized nonepistatic selection forms operating in multilocus multiallele systems. The selection structure admits a natural representation as simple sums of Kronecker products based on a common set of marginal selection components. A flexible parametrization of the recombination process is introduced leading to a canonical derivation of the transformation equations connecting gamete frequency states over successive generations. Conditions for the existence and stability of multilocus Hardy-Weinberg (H.W.) type equilibria are elaborated for the classical nonepistatic models (multiplicative and additive viability effects across loci) as well as for generalized nonepistatic selection expressions. It is established that the range of recombination distributions maintaining a stable H.W. polymorphic equilibrium is confined to loose linkage in the pure multiplicative case, but is not restricted in the additive model. In the bisexual case we ascertain for the generalized nonepistatic model the stability conditions of a common H.W polymorphism."}
{"id": "PMID:469415", "title": "Some analytical results about a simple reaction-diffusion system for morphogenesis.", "content": "The reaction-diffusion system considered involves only one nonlinear term and is a gradient system. In a bifurcation analysis for the equilibrium states, the global existence of infinitely many solution branches can be shown by the method of Ljusternik-Schnirelmann. Their stability is studied. Using a Ljapunov functional it can be shown that the solutions of the time-dependent system converge to the equilibrium states.", "contents": "Some analytical results about a simple reaction-diffusion system for morphogenesis. The reaction-diffusion system considered involves only one nonlinear term and is a gradient system. In a bifurcation analysis for the equilibrium states, the global existence of infinitely many solution branches can be shown by the method of Ljusternik-Schnirelmann. Their stability is studied. Using a Ljapunov functional it can be shown that the solutions of the time-dependent system converge to the equilibrium states."}
{"id": "PMID:469416", "title": "A simple model of delay, block and one way conduction in Purkinje fibers.", "content": "A simple qualitative model of the cardiac Purkinje fiber is introduced for the purpose of numerical simulation of experiments on conduction of the cardiac impulse. Well known approximation techniques are used to illustrate the behavior of the model membrane in the cases of space clamp and propagation of traveling pulses in a uniform infinite fiber. The results of the numerical simulations are then presented, and shown to be comparable to experiment.", "contents": "A simple model of delay, block and one way conduction in Purkinje fibers. A simple qualitative model of the cardiac Purkinje fiber is introduced for the purpose of numerical simulation of experiments on conduction of the cardiac impulse. Well known approximation techniques are used to illustrate the behavior of the model membrane in the cases of space clamp and propagation of traveling pulses in a uniform infinite fiber. The results of the numerical simulations are then presented, and shown to be comparable to experiment."}
{"id": "PMID:469417", "title": "A new mathematical approach for solving carrier-facilitated steady-state diffusion problems.", "content": "A new mathematical treatment is presented which simplifies the solution of carrier-diffusion problems. The method is generally applicable and is illustrated and tested for a specific, commonly occurring situation: facilitated diffusion of a single substrate through flat layers. Results predicted for total substrate flux are in excellent agreement with control computer calculations. The method also can be used to obtain concentration profiles for each species; here the results are good only if conditions at the boundaries are predicted correctly.", "contents": "A new mathematical approach for solving carrier-facilitated steady-state diffusion problems. A new mathematical treatment is presented which simplifies the solution of carrier-diffusion problems. The method is generally applicable and is illustrated and tested for a specific, commonly occurring situation: facilitated diffusion of a single substrate through flat layers. Results predicted for total substrate flux are in excellent agreement with control computer calculations. The method also can be used to obtain concentration profiles for each species; here the results are good only if conditions at the boundaries are predicted correctly."}
{"id": "PMID:469418", "title": "On the kinetics of lead in the human body.", "content": "A compartment model is derived for the kinetics of lead in the human body. The parameters are estimated from field data. Numerical solutions of the equations are obtained. They are used to discuss recent theories on lead intake, in particular from the atmosphere.", "contents": "On the kinetics of lead in the human body. A compartment model is derived for the kinetics of lead in the human body. The parameters are estimated from field data. Numerical solutions of the equations are obtained. They are used to discuss recent theories on lead intake, in particular from the atmosphere."}
{"id": "PMID:469440", "title": "Limitations of exercise testing in critical left coronary artery disease.", "content": "Exercise testing was evaluated in 51 patients with critical left coronary artery disease (LCA) documented by coronary arteriography within two weeks of their bicycle ergometer (26 patients) or treadmill (25 patients) electrocardiographic study. Adequate tests, as defined by the patient having reached 85% of predicted maximum heart rate, were achieved in only 16 patients on the ergometer (62%) and in 21 (84%) on the treadmill. Nevertheless mean maximum double product (220 vs 232) and mean exercise time (4.6 min vs 5.0 min.) were similar (p:NS). The sensitivity for ergometry and treadmill testing was 75 and 62% respectively. If however we include those negative studies in which for various reasons patients were unable to achieve 85% of their predicted maximal heart rate (i.e., inadequate studies), sensitivity was only 46 and 52% respectively. Thus a sizable group of patients with critical LCA disease cannot adequately perform exercise tests especially on the ergometer; and over half of all such patients studied will be found for one reason or another to respond negatively, regardless of exercise protocol.", "contents": "Limitations of exercise testing in critical left coronary artery disease. Exercise testing was evaluated in 51 patients with critical left coronary artery disease (LCA) documented by coronary arteriography within two weeks of their bicycle ergometer (26 patients) or treadmill (25 patients) electrocardiographic study. Adequate tests, as defined by the patient having reached 85% of predicted maximum heart rate, were achieved in only 16 patients on the ergometer (62%) and in 21 (84%) on the treadmill. Nevertheless mean maximum double product (220 vs 232) and mean exercise time (4.6 min vs 5.0 min.) were similar (p:NS). The sensitivity for ergometry and treadmill testing was 75 and 62% respectively. If however we include those negative studies in which for various reasons patients were unable to achieve 85% of their predicted maximal heart rate (i.e., inadequate studies), sensitivity was only 46 and 52% respectively. Thus a sizable group of patients with critical LCA disease cannot adequately perform exercise tests especially on the ergometer; and over half of all such patients studied will be found for one reason or another to respond negatively, regardless of exercise protocol."}
{"id": "PMID:469441", "title": "Electrocardiographic changes in thyrotoxic periodic paralysis.", "content": "The electrocardiograms (ECGs) of 30 patients with hypokalaemic thyrotoxic periodic paralysis during and after paralysis were studied. During paralysis, typical features of hypokalaemia were seen in all patients with serum potassium levels of 2.8 mmol/l or less; above this level, the ECGs varied from non-diagnostic to those showing typical features of hypokalaemia. It was not possible to accurately predict the serum potassium level from the ECG except when either sinus arrest or heart block was present. Although extrasystoles have been reported to be common in hypokalaemia, none of the patients in this study had extrasystoles. Sinus arrest occurred in two patients and second degree atrio-ventricular block occurred in three patients, a finding which has not been reported in hypokalaemia.", "contents": "Electrocardiographic changes in thyrotoxic periodic paralysis. The electrocardiograms (ECGs) of 30 patients with hypokalaemic thyrotoxic periodic paralysis during and after paralysis were studied. During paralysis, typical features of hypokalaemia were seen in all patients with serum potassium levels of 2.8 mmol/l or less; above this level, the ECGs varied from non-diagnostic to those showing typical features of hypokalaemia. It was not possible to accurately predict the serum potassium level from the ECG except when either sinus arrest or heart block was present. Although extrasystoles have been reported to be common in hypokalaemia, none of the patients in this study had extrasystoles. Sinus arrest occurred in two patients and second degree atrio-ventricular block occurred in three patients, a finding which has not been reported in hypokalaemia."}
{"id": "PMID:469442", "title": "The effect of pregnancy on the frontal plane QRS axis.", "content": "The electrical axis of the heart is an important parameter in electrocardiographic assessment. Slight degrees of left axis deviation have been attributed to a horizontal heart position resulting from the mechanical effects of abdominal distension, such as the physiological event of pregnancy. This study was undertaken to establish the validity of this concept.", "contents": "The effect of pregnancy on the frontal plane QRS axis. The electrical axis of the heart is an important parameter in electrocardiographic assessment. Slight degrees of left axis deviation have been attributed to a horizontal heart position resulting from the mechanical effects of abdominal distension, such as the physiological event of pregnancy. This study was undertaken to establish the validity of this concept."}
{"id": "PMID:469443", "title": "Cardiac electrophysiologic effects of etafenone hydrochloride (Dialicor), a new antiarrhythmic agent.", "content": "The electrophysiologic effects of etafenone hydrochloride (Dialicor) were studied in isolated rabbit hearts and canine or rabbit cardiac tissues with intracellular microelectrode techniques. The concentration range used was 0.25-10 mg/L, except in experiments with direct addition of etafenone into the tissue bath. Etafenone depressed automaticity in the sinus node, atrioventricular junction and Purkinje fibers at concentrations not affecting the action potential contour. In both atrial and ventricular fibers, etafenone prolonged the action potential duration and total refractory period. The action potential upstroke was slowed and conduction time increased. Higher concentrations reduced the resting potential, causing incomplete depolarization. Atrial action potentials were affected at lower concentrations than ventricular and Purkinje action potentials. Occasi-nally, etafenone produced localized conduction block within the atria, usually sparing internodal conduction. In electrically driven hearts, direct recording of His bundle potential as well as surface electrograms revealed marked prolongation of intraatrial and intranodal conduction times, with some increase in His-Purkinje and ventricular conduction times. Depression of atrioventricular conduction was insignificant under sinus rhythm. Possible therapeutic implications of these electrophysiologic effects are discussed.", "contents": "Cardiac electrophysiologic effects of etafenone hydrochloride (Dialicor), a new antiarrhythmic agent. The electrophysiologic effects of etafenone hydrochloride (Dialicor) were studied in isolated rabbit hearts and canine or rabbit cardiac tissues with intracellular microelectrode techniques. The concentration range used was 0.25-10 mg/L, except in experiments with direct addition of etafenone into the tissue bath. Etafenone depressed automaticity in the sinus node, atrioventricular junction and Purkinje fibers at concentrations not affecting the action potential contour. In both atrial and ventricular fibers, etafenone prolonged the action potential duration and total refractory period. The action potential upstroke was slowed and conduction time increased. Higher concentrations reduced the resting potential, causing incomplete depolarization. Atrial action potentials were affected at lower concentrations than ventricular and Purkinje action potentials. Occasi-nally, etafenone produced localized conduction block within the atria, usually sparing internodal conduction. In electrically driven hearts, direct recording of His bundle potential as well as surface electrograms revealed marked prolongation of intraatrial and intranodal conduction times, with some increase in His-Purkinje and ventricular conduction times. Depression of atrioventricular conduction was insignificant under sinus rhythm. Possible therapeutic implications of these electrophysiologic effects are discussed."}
{"id": "PMID:469445", "title": "Increasing pre-excitation during exercise and isoproterenol infusion. Evidence for a catecholamine sensitive bypass tract.", "content": "A patient with atypical chest pain developed pre-excitation during exercise and isoproterenol infusion, with \"ischemic\" ST depression only during the pre-excited beats. Coronary angiography and myocardial lactate extraction showed no evidence of abnormal coronary vessels. Electrophysiologic study and pacing-induced tachycardia did not induce pre-excitation, whereas exercise induced progressive increase in pre-excitation. The data are consistent with an unusual form of pre-excitation, perhaps related to responsiveness of an accessory bypass tract to catecholamine stimulation. The data also show that the false positive exercise test in this syndrome is due to progressively increasing pre-excitation and therefore more abnormal repolarization.", "contents": "Increasing pre-excitation during exercise and isoproterenol infusion. Evidence for a catecholamine sensitive bypass tract. A patient with atypical chest pain developed pre-excitation during exercise and isoproterenol infusion, with \"ischemic\" ST depression only during the pre-excited beats. Coronary angiography and myocardial lactate extraction showed no evidence of abnormal coronary vessels. Electrophysiologic study and pacing-induced tachycardia did not induce pre-excitation, whereas exercise induced progressive increase in pre-excitation. The data are consistent with an unusual form of pre-excitation, perhaps related to responsiveness of an accessory bypass tract to catecholamine stimulation. The data also show that the false positive exercise test in this syndrome is due to progressively increasing pre-excitation and therefore more abnormal repolarization."}
{"id": "PMID:469446", "title": "Treatment of heart block due to sarcoid heart disease.", "content": "The role of glucocorticosteroid therapy for myocardial sarcoidosis is not well defined. This report shows the effect of prednisone therapy on atrioventricular (AV) conduction in a patient with myocardial sarcoidosis and AV block. On three separate occasions AV block was documented prior to prednisone therapy. On the first two occasions the patient had first and second degree AV block which by His bundle electrogram initially was shown to be in the AV node. On the third occasion the patient developed complete heart block. On each occasion treatment with prednisone resulted in improved AV conduction. The results indicate that prednisone therapy can be beneficial in the treatment of AV block due to myocardial sarcoidosis.", "contents": "Treatment of heart block due to sarcoid heart disease. The role of glucocorticosteroid therapy for myocardial sarcoidosis is not well defined. This report shows the effect of prednisone therapy on atrioventricular (AV) conduction in a patient with myocardial sarcoidosis and AV block. On three separate occasions AV block was documented prior to prednisone therapy. On the first two occasions the patient had first and second degree AV block which by His bundle electrogram initially was shown to be in the AV node. On the third occasion the patient developed complete heart block. On each occasion treatment with prednisone resulted in improved AV conduction. The results indicate that prednisone therapy can be beneficial in the treatment of AV block due to myocardial sarcoidosis."}
{"id": "PMID:469447", "title": "Congenital complete heart block and long Q-T syndrome requiring ventricular pacing for control of refractory ventricular tachycardia and fibrillation.", "content": "A three-year-old girl with congenital complete heart block presented with repeated bouts of ventricular tachycardia and ventricular fibrillation. The ECG was remarkable for both complete heart block and a long Q-T interval, when corrected for rate. The Q-T interval was longer than the Q-T interval of children with congenital complete heart block and of children without heart disease. Overdrive ventricular pacing was necessary to control the arrhythmias. A prolonged Q-T interval in patients with complete heart block, even in the presence of a normal QRS duration, may predispose the patient to sudden death. Permanent pacing can suppress these arrhythmias by overdriving.", "contents": "Congenital complete heart block and long Q-T syndrome requiring ventricular pacing for control of refractory ventricular tachycardia and fibrillation. A three-year-old girl with congenital complete heart block presented with repeated bouts of ventricular tachycardia and ventricular fibrillation. The ECG was remarkable for both complete heart block and a long Q-T interval, when corrected for rate. The Q-T interval was longer than the Q-T interval of children with congenital complete heart block and of children without heart disease. Overdrive ventricular pacing was necessary to control the arrhythmias. A prolonged Q-T interval in patients with complete heart block, even in the presence of a normal QRS duration, may predispose the patient to sudden death. Permanent pacing can suppress these arrhythmias by overdriving."}
{"id": "PMID:469452", "title": "Comparison of prolactin levels in human semen and seminal plasma.", "content": "Semen samples were collected from 35 men and the levels of prolactin in semen and seminal plasma were measured. There was no significant difference in prolactin concentrations between the two fluids (t = 0.333, P greater than 0.7). There was also no correlation between the prolactin concentration and the kinematic viscosity of the semen (r = 0.065, P greater than 0.7).", "contents": "Comparison of prolactin levels in human semen and seminal plasma. Semen samples were collected from 35 men and the levels of prolactin in semen and seminal plasma were measured. There was no significant difference in prolactin concentrations between the two fluids (t = 0.333, P greater than 0.7). There was also no correlation between the prolactin concentration and the kinematic viscosity of the semen (r = 0.065, P greater than 0.7)."}
{"id": "PMID:469453", "title": "Ultradian and circadian rhythms in the plasma concentration of cortisol in sheep.", "content": "Three Merino ewes, adapted for about 3 weeks to their environment, were bled at 10 min intervals through a jugular venous cannula. Radioimmunoassay of plasma samples for cortisol revealed marked diurnal variations with peak levels just after midnight and lowest values in the afternoon. This rhythm appeared to result from a changing amplitude associated with a distinct ultradian rhythm (frequency 0.8-1.2 cycles/h) in the plasma level of cortisol. Calculation of the daily rate of secretion of cortisol from the hormone profiles gave a mean value of 8.49 mg. Arguments are put forward in favour of this method for obtaining the true rate of secretion of cortisol.", "contents": "Ultradian and circadian rhythms in the plasma concentration of cortisol in sheep. Three Merino ewes, adapted for about 3 weeks to their environment, were bled at 10 min intervals through a jugular venous cannula. Radioimmunoassay of plasma samples for cortisol revealed marked diurnal variations with peak levels just after midnight and lowest values in the afternoon. This rhythm appeared to result from a changing amplitude associated with a distinct ultradian rhythm (frequency 0.8-1.2 cycles/h) in the plasma level of cortisol. Calculation of the daily rate of secretion of cortisol from the hormone profiles gave a mean value of 8.49 mg. Arguments are put forward in favour of this method for obtaining the true rate of secretion of cortisol."}
{"id": "PMID:469455", "title": "Effect of storage on the bioactivity of two international reference preparations of human pituitary luteinizing hormone in vitro.", "content": "There was no loss of biological activity in vitro when the 1st International Reference Preparation (IRP) for human pituitary gonadotrophins [FSH and LH/interstitial cell stimulating hormone (ICSH) for bioassay] code no. 69/104 and the 1st IRP for human pituitary LH/ICSH [for immunoassay] code no. 68/40 were stored for 1 year at -70 degrees C in a buffered 0.8% saline solution containing 1% bovine plasma albumin (BPA). However, storage of the 69/104 preparation at -20 degrees C in either 0.1 or 1% BPA, or at -70 degrees C in the presence of 0.1% BPA showed a small but significant decrease (approximately 10%) in activity over the same period. It is, therefore, advantageous to store these reference preparations at -70 degrees C in the presence of 1% BPA.", "contents": "Effect of storage on the bioactivity of two international reference preparations of human pituitary luteinizing hormone in vitro. There was no loss of biological activity in vitro when the 1st International Reference Preparation (IRP) for human pituitary gonadotrophins [FSH and LH/interstitial cell stimulating hormone (ICSH) for bioassay] code no. 69/104 and the 1st IRP for human pituitary LH/ICSH [for immunoassay] code no. 68/40 were stored for 1 year at -70 degrees C in a buffered 0.8% saline solution containing 1% bovine plasma albumin (BPA). However, storage of the 69/104 preparation at -20 degrees C in either 0.1 or 1% BPA, or at -70 degrees C in the presence of 0.1% BPA showed a small but significant decrease (approximately 10%) in activity over the same period. It is, therefore, advantageous to store these reference preparations at -70 degrees C in the presence of 1% BPA."}
{"id": "PMID:469456", "title": "Functional luteolysis in the pseudopregnant rat: effects of prostaglandin F2 alpha and 16-aryloxy prostaglandin F2 alpha in vitro.", "content": "The present work concerns the luteolytic effects of prostaglandin (PG) F2 alpha and its analogue, 16-aryloxy PGF2 alpha, upon isolated luteal cells. Varying doses of these two prostaglandins were incubated with cells in the presence or absence of an optimum stimulatory dose of LH (1 microgram/ml). The total contents of progesterone and 20 alpha-dihydroprogesterone in flasks were determined after the incubation periods by radioimmunoassay. Both prostaglandins inhibited basal synthesis of progesterone and 20 alpha-dihydroprogesterone, maximum inhibition occurring at concentrations of either PG of between 250 and 500 ng/ml. In this dose range both prostaglandins were found to abolish LH-stimulated progestogen synthesis completely. These effects were discernible within 5 min of incubation. The studies demonstrated that the onset of PG-induced luteolysis in vitro is characterized by an inhibition of the biosynthesis of both progesterone and its weakly progestogenic metabolite, 20 alpha-dihydroprogesterone; induction of 20 alpha-hydroxysteroid dehydrogenase activity by either PG was not found in incubations extending up to 60 min. In contrast to their relative potencies in vivo, PGF2 alpha and 16-aryloxy PGF2 alpha were essentially equipotent in this in-vitro system.", "contents": "Functional luteolysis in the pseudopregnant rat: effects of prostaglandin F2 alpha and 16-aryloxy prostaglandin F2 alpha in vitro. The present work concerns the luteolytic effects of prostaglandin (PG) F2 alpha and its analogue, 16-aryloxy PGF2 alpha, upon isolated luteal cells. Varying doses of these two prostaglandins were incubated with cells in the presence or absence of an optimum stimulatory dose of LH (1 microgram/ml). The total contents of progesterone and 20 alpha-dihydroprogesterone in flasks were determined after the incubation periods by radioimmunoassay. Both prostaglandins inhibited basal synthesis of progesterone and 20 alpha-dihydroprogesterone, maximum inhibition occurring at concentrations of either PG of between 250 and 500 ng/ml. In this dose range both prostaglandins were found to abolish LH-stimulated progestogen synthesis completely. These effects were discernible within 5 min of incubation. The studies demonstrated that the onset of PG-induced luteolysis in vitro is characterized by an inhibition of the biosynthesis of both progesterone and its weakly progestogenic metabolite, 20 alpha-dihydroprogesterone; induction of 20 alpha-hydroxysteroid dehydrogenase activity by either PG was not found in incubations extending up to 60 min. In contrast to their relative potencies in vivo, PGF2 alpha and 16-aryloxy PGF2 alpha were essentially equipotent in this in-vitro system."}
{"id": "PMID:469457", "title": "Androgen receptors in the rat brain, anterior pituitary gland and ventral prostate gland: effects of orchidectomy and ageing.", "content": "Available high-affinity binding sites for 5 alpha-dihydrotestosterone (DHT) were measured in cytosols obtained from the amygdala, hypothalamus, anterior pituitary gland and ventral prostate gland of 12-week-old rats at various times after orchidectomy, and in the corresponding tissues of 18-month-old male rats. It is suggested that the lower affinity of the DHT binding reaction in brain and ventral prostatic cytosols after orchidectomy or ageing respectively, may explain, at least in part, the changes in the responsiveness of the tissues to androgens.", "contents": "Androgen receptors in the rat brain, anterior pituitary gland and ventral prostate gland: effects of orchidectomy and ageing. Available high-affinity binding sites for 5 alpha-dihydrotestosterone (DHT) were measured in cytosols obtained from the amygdala, hypothalamus, anterior pituitary gland and ventral prostate gland of 12-week-old rats at various times after orchidectomy, and in the corresponding tissues of 18-month-old male rats. It is suggested that the lower affinity of the DHT binding reaction in brain and ventral prostatic cytosols after orchidectomy or ageing respectively, may explain, at least in part, the changes in the responsiveness of the tissues to androgens."}
{"id": "PMID:469458", "title": "Testosterone 5 alpha-reductase activity as related to proliferative status in mouse accessory sex glands.", "content": "Testosterone 5 alpha-reductase activity has been measured during the proliferative response of the seminal vesicle and coagulating gland of the castrated mouse after continuous treatment with tesotsterone. Daily subcutaneous injections were begun either 3 or 14 days after castration. At 3 days after castration, a pre-replicative period of 50-60 h occurred before the onset of DNA synthesis, and a continuous [3H]thymidine labelling procedure revealed that only a minority of epithelial cells were subsequently involved in the transitory proliferative response. At 14 days after castration, cells were able to prepare more quickly for DNA synthesis (20-30 h), and the subsequent proliferative response involved a higher proportion of the epithelial cells present. At 14 days after castration, testosterone treatment resulted in the activity of the enzyme/mg homogenate increasing fourfold in the seminal vesicle and tenfold in the coagulating gland, to values almost double those of control (intact animals. At 3 days after castration, testosterone treatment resulted in much slower increases in enzyme activity, and at the completion of day 5 of treatment, levels were close to control values. It is concluded that a relationship may exist between the initial increase in 5 alpha-reductase activity and the duration of the pre-replicative period before DNA synthesis begins. Although testosterone and its resultant effects upon 5 alpha-reductase activity are clearly implicated in the genesis of the proliferative response, the ultimate control of cell proliferation must be independent of androgenic hormones. When cell division was effectively curtailed at the completion of day 4 of testosterone treatment, abnormally high levels of enzyme activity were still present.", "contents": "Testosterone 5 alpha-reductase activity as related to proliferative status in mouse accessory sex glands. Testosterone 5 alpha-reductase activity has been measured during the proliferative response of the seminal vesicle and coagulating gland of the castrated mouse after continuous treatment with tesotsterone. Daily subcutaneous injections were begun either 3 or 14 days after castration. At 3 days after castration, a pre-replicative period of 50-60 h occurred before the onset of DNA synthesis, and a continuous [3H]thymidine labelling procedure revealed that only a minority of epithelial cells were subsequently involved in the transitory proliferative response. At 14 days after castration, cells were able to prepare more quickly for DNA synthesis (20-30 h), and the subsequent proliferative response involved a higher proportion of the epithelial cells present. At 14 days after castration, testosterone treatment resulted in the activity of the enzyme/mg homogenate increasing fourfold in the seminal vesicle and tenfold in the coagulating gland, to values almost double those of control (intact animals. At 3 days after castration, testosterone treatment resulted in much slower increases in enzyme activity, and at the completion of day 5 of treatment, levels were close to control values. It is concluded that a relationship may exist between the initial increase in 5 alpha-reductase activity and the duration of the pre-replicative period before DNA synthesis begins. Although testosterone and its resultant effects upon 5 alpha-reductase activity are clearly implicated in the genesis of the proliferative response, the ultimate control of cell proliferation must be independent of androgenic hormones. When cell division was effectively curtailed at the completion of day 4 of testosterone treatment, abnormally high levels of enzyme activity were still present."}
{"id": "PMID:469459", "title": "Retention of plasma somatomedin activity in the foetal rabbit following decapitation in utero.", "content": "One rabbit foetus within each litter was decapitated in utero on day 24 of gestation. Plasma somatomedin activity and costal cartilage metabolism were studied 5 days later in the experimental foetuses and control litter-mates. Somatomedin was assayed by the uptake of [35S]sulphate in vitro into costal cartilage from intact foetuses. Uptake was proportional to logarithmic increases in the concentration of both foetal and maternal rabbit plasma. The mean (+/- 1 S.D.) somatomedin activity of four plasma pools, each pool being derived from the intact foetuses within each of four litters, was 1.3 +/- 0.3 compared with a potency of unity for the reference pool of maternal plasma. The plasma somatomedin activity of decapitated foetuses did not differ significantly from that of control litter-mates when analysed by rank test, but the costal cartilage of decapitated foetuses took up less [35S]-sulphate in basal medium when compared with that of intact litter-mates. The headless body weight of the decapitated foetueses did not rank in a position significantly different from the one expected. The concentration of plasma growth hormone in the decapitated foetuses was less than 5 ng/ml and that of the intact foetuses was more than 157 ng/ml. It is concluded that plasma somatomedin in the rabbit foetus is not dependent on foetal growth hormone.", "contents": "Retention of plasma somatomedin activity in the foetal rabbit following decapitation in utero. One rabbit foetus within each litter was decapitated in utero on day 24 of gestation. Plasma somatomedin activity and costal cartilage metabolism were studied 5 days later in the experimental foetuses and control litter-mates. Somatomedin was assayed by the uptake of [35S]sulphate in vitro into costal cartilage from intact foetuses. Uptake was proportional to logarithmic increases in the concentration of both foetal and maternal rabbit plasma. The mean (+/- 1 S.D.) somatomedin activity of four plasma pools, each pool being derived from the intact foetuses within each of four litters, was 1.3 +/- 0.3 compared with a potency of unity for the reference pool of maternal plasma. The plasma somatomedin activity of decapitated foetuses did not differ significantly from that of control litter-mates when analysed by rank test, but the costal cartilage of decapitated foetuses took up less [35S]-sulphate in basal medium when compared with that of intact litter-mates. The headless body weight of the decapitated foetueses did not rank in a position significantly different from the one expected. The concentration of plasma growth hormone in the decapitated foetuses was less than 5 ng/ml and that of the intact foetuses was more than 157 ng/ml. It is concluded that plasma somatomedin in the rabbit foetus is not dependent on foetal growth hormone."}
{"id": "PMID:469460", "title": "Specificity of radioimmunoassays for relaxin.", "content": "The specificities of two radioimmunoassays (RIA) for relaxin, based upon crude porcine relaxin (NIH-R-P1; RIA I) and a highly purified porcine relaxin (RIA II) have been studied concurrently using purified hormones and plasma samples. A labelled fraction, selected from radio-iodinated NIH-R-P1 and used in that RIA, was also bound to antiserum raised to the highly purified relaxin. Hence a third RIA was possible in which both the crude and the purified relaxins inhibited in the ng/ml range. Porcine insulin and the connecting peptide of porcine proinsulin did not inhibit any of the assay systems whereas porcine proinsulin did inhibit in each assay at the microgram/ml range. Concurrent measurements by assays I and II have been made in sheep plasma obtained during both delivery of the lamb and suckling. The peak values obtained by assays I and II are 3 and 6 min out of phase during suckling and delivery respectively; the NIH-R-P1 relaxin immunoactivity appearing first. The plasma inhibition curves of both appear to be the sum of individual contributions from relaxin and relaxin-like peptides, such as prorelaxin and its fragments, as seen by different antisera. Both assays, however, give qualitatively similar indices of relaxin immunoactivity. The RIA developed for the more purified peptide would be expected to yield a better quantitative estimate of relaxin secretion but this, like specificity, cannot be shown absolutely.", "contents": "Specificity of radioimmunoassays for relaxin. The specificities of two radioimmunoassays (RIA) for relaxin, based upon crude porcine relaxin (NIH-R-P1; RIA I) and a highly purified porcine relaxin (RIA II) have been studied concurrently using purified hormones and plasma samples. A labelled fraction, selected from radio-iodinated NIH-R-P1 and used in that RIA, was also bound to antiserum raised to the highly purified relaxin. Hence a third RIA was possible in which both the crude and the purified relaxins inhibited in the ng/ml range. Porcine insulin and the connecting peptide of porcine proinsulin did not inhibit any of the assay systems whereas porcine proinsulin did inhibit in each assay at the microgram/ml range. Concurrent measurements by assays I and II have been made in sheep plasma obtained during both delivery of the lamb and suckling. The peak values obtained by assays I and II are 3 and 6 min out of phase during suckling and delivery respectively; the NIH-R-P1 relaxin immunoactivity appearing first. The plasma inhibition curves of both appear to be the sum of individual contributions from relaxin and relaxin-like peptides, such as prorelaxin and its fragments, as seen by different antisera. Both assays, however, give qualitatively similar indices of relaxin immunoactivity. The RIA developed for the more purified peptide would be expected to yield a better quantitative estimate of relaxin secretion but this, like specificity, cannot be shown absolutely."}
{"id": "PMID:469461", "title": "Metabolism of prolactin in mice with a high incidence of mammary tumours: evidence for greater conversion into a non-immunoassayable form.", "content": "Monomeric mouse prolactin containing small amounts of 125I-labelled prolactin was administered to adult female mice of a high (C3H/St) and low (C57BL/St) mammary tumour strain. Their endogenous prolactin had been suppressed with 2-bromo-alpha-ergocryptine. The chromatographic profile, on Sephadex G-100, of prolactin in the serum of mice injected with mouse prolactin was compared by direct measurement (radioactivity count) and by radioimmunoassay (RIA) at several intervals after injection. With both methods, the injected hormone was found in the serum in predominantly two molecular sizes, the so-called 'big' and 'little' forms. Although 'little' prolactin in both strains constituted a constant 80% of the total hormone at most intervals by direct measurement, it comprised a comparatively smaller proportion by RIA. In addition, the RIA-determined 'little' prolactin, after reaching maximum levels at 15 min, progressively decreased with time, the decrease being greater in the C3H/St than in the C57BL/St strain. Similar experiments with mouse growth hormone revealed no such discrepancies between the radioactivity counts and the RIA measurements. A fraction of both 'big' and 'little' forms in the C3H/St strain failed to precipitate completely after the material had been incubated with an antiserum to mouse prolactin. These results demonstrate that the prolactin injected into mice is metabolized in serum into two non-immunoreactive forms, one that elutes with the same elution volume on Sephadex G-100 column as the monomer and the other that elutes as the 'big' form. Furthermore, the loss of immunoreactivity of monomeric mouse prolactin is greater in the high-tumour C3H/St strain than in the low-tumour C57BL/St strain. Endogenous immunoreactive prolactin, on the other hand, was found mainly in the 'big' form in the serum of female mice of the C3H/St strain under basal conditions, whereas it was present only in the 'little' form in comparable mice of the C57BL/St strain, even though pituitary extracts of both strains contained mainly the 'little' form. These results support the concept that monomeric prolactin in the systemic circulation of the tumour-prone C3H/St strain is largely in a non-immunoreactive form.", "contents": "Metabolism of prolactin in mice with a high incidence of mammary tumours: evidence for greater conversion into a non-immunoassayable form. Monomeric mouse prolactin containing small amounts of 125I-labelled prolactin was administered to adult female mice of a high (C3H/St) and low (C57BL/St) mammary tumour strain. Their endogenous prolactin had been suppressed with 2-bromo-alpha-ergocryptine. The chromatographic profile, on Sephadex G-100, of prolactin in the serum of mice injected with mouse prolactin was compared by direct measurement (radioactivity count) and by radioimmunoassay (RIA) at several intervals after injection. With both methods, the injected hormone was found in the serum in predominantly two molecular sizes, the so-called 'big' and 'little' forms. Although 'little' prolactin in both strains constituted a constant 80% of the total hormone at most intervals by direct measurement, it comprised a comparatively smaller proportion by RIA. In addition, the RIA-determined 'little' prolactin, after reaching maximum levels at 15 min, progressively decreased with time, the decrease being greater in the C3H/St than in the C57BL/St strain. Similar experiments with mouse growth hormone revealed no such discrepancies between the radioactivity counts and the RIA measurements. A fraction of both 'big' and 'little' forms in the C3H/St strain failed to precipitate completely after the material had been incubated with an antiserum to mouse prolactin. These results demonstrate that the prolactin injected into mice is metabolized in serum into two non-immunoreactive forms, one that elutes with the same elution volume on Sephadex G-100 column as the monomer and the other that elutes as the 'big' form. Furthermore, the loss of immunoreactivity of monomeric mouse prolactin is greater in the high-tumour C3H/St strain than in the low-tumour C57BL/St strain. Endogenous immunoreactive prolactin, on the other hand, was found mainly in the 'big' form in the serum of female mice of the C3H/St strain under basal conditions, whereas it was present only in the 'little' form in comparable mice of the C57BL/St strain, even though pituitary extracts of both strains contained mainly the 'little' form. These results support the concept that monomeric prolactin in the systemic circulation of the tumour-prone C3H/St strain is largely in a non-immunoreactive form."}
{"id": "PMID:469462", "title": "Non-selective inhibition of basal glucagon release by [D-Cys14]-analogues of somatostatin in the rat.", "content": "The effects of two [D-Cys14]-analogues of somatostatin on basal plasma levels of glucagon, insulin and glucose were determined in unanaesthetized rats to re-examine a glucagon-selective action of these peptides which has been claimed by others. Somatostatin, [D-Cys14]-somatostatin and [D-Trp8, D-Cys14]-somatostatin caused a short-lasting, dose-dependent decrease of plasma glucagon and insulin but they had no significant influence on plasma glucose. Glucagon and insulin reached the nadir 2 min after intravenous injection of the peptides (dose range 1--10 micrograms/kg) or 5 min after subcutaneous administration (30 and 300 micrograms/kg). At the nadir, insulin was decreased to a greater extent than glucagon and the effecer the nadir and at high doses, the time-course of some effects of the analogues on either glucagon or insulin differed from that of somatostatin. Thus, these [D-Cys14]-analogues may show partial kinetic dissociation of effects on glucagon and insulin but they are not truly selective inhibitors of glucagon release.", "contents": "Non-selective inhibition of basal glucagon release by [D-Cys14]-analogues of somatostatin in the rat. The effects of two [D-Cys14]-analogues of somatostatin on basal plasma levels of glucagon, insulin and glucose were determined in unanaesthetized rats to re-examine a glucagon-selective action of these peptides which has been claimed by others. Somatostatin, [D-Cys14]-somatostatin and [D-Trp8, D-Cys14]-somatostatin caused a short-lasting, dose-dependent decrease of plasma glucagon and insulin but they had no significant influence on plasma glucose. Glucagon and insulin reached the nadir 2 min after intravenous injection of the peptides (dose range 1--10 micrograms/kg) or 5 min after subcutaneous administration (30 and 300 micrograms/kg). At the nadir, insulin was decreased to a greater extent than glucagon and the effecer the nadir and at high doses, the time-course of some effects of the analogues on either glucagon or insulin differed from that of somatostatin. Thus, these [D-Cys14]-analogues may show partial kinetic dissociation of effects on glucagon and insulin but they are not truly selective inhibitors of glucagon release."}
{"id": "PMID:469463", "title": "Serum levels of the acid-ethanol soluble component of non-suppressible insulin-like activity in untreated and treated streptozotocin-diabetic rats.", "content": "Streptozotocin-diabetic rats suffered growth failure and had reduced serum levels of the acid--ethanol soluble component of non-suppressible insulin-like activity (NSILA-S) compared with normal rats. Chronic insulin substitution (6 weeks) resulted in a normalization of serum levels of NSILA-S; this was accompanied by a normal increase in weight. Insulin therapy for 3 days resulted in a partial recovery of serum levels of NSILA-S and a slight but significant accompanying gain in weight. Short-term administration of GH also resulted in a partial recovery of the serum level of NSILA-S, in spite of continued uncontrolled diabetes. These results demonstrate that, in the rat, insulin as well as GH contributes to the regulation of serum levels of NSILA-S.", "contents": "Serum levels of the acid-ethanol soluble component of non-suppressible insulin-like activity in untreated and treated streptozotocin-diabetic rats. Streptozotocin-diabetic rats suffered growth failure and had reduced serum levels of the acid--ethanol soluble component of non-suppressible insulin-like activity (NSILA-S) compared with normal rats. Chronic insulin substitution (6 weeks) resulted in a normalization of serum levels of NSILA-S; this was accompanied by a normal increase in weight. Insulin therapy for 3 days resulted in a partial recovery of serum levels of NSILA-S and a slight but significant accompanying gain in weight. Short-term administration of GH also resulted in a partial recovery of the serum level of NSILA-S, in spite of continued uncontrolled diabetes. These results demonstrate that, in the rat, insulin as well as GH contributes to the regulation of serum levels of NSILA-S."}
{"id": "PMID:469464", "title": "Conditional discrimination after errorless and trial-and-error training.", "content": "Children were trained on a visual discrimination by stimulus shaping, stimulus fading, or trial-and-error. Those who did not acquire the conditional discrimination received a second, different training. More children initially trained by stimulus shaping acquired the conditional discrimination than did those initially trained with stimulus fading or trial-and-error. After a history of fading or trial-and-error training, children were less likely to acquire the conditional discrimination even after the more successful procedure of shaping was later used.", "contents": "Conditional discrimination after errorless and trial-and-error training. Children were trained on a visual discrimination by stimulus shaping, stimulus fading, or trial-and-error. Those who did not acquire the conditional discrimination received a second, different training. More children initially trained by stimulus shaping acquired the conditional discrimination than did those initially trained with stimulus fading or trial-and-error. After a history of fading or trial-and-error training, children were less likely to acquire the conditional discrimination even after the more successful procedure of shaping was later used."}
{"id": "PMID:469465", "title": "Attention in the pigeon: testing for excitatory and inhibitory control by the weak elements.", "content": "In two experiments, pigeons were trained on a successive discrimination between a color and either a compound S+ or a compound S- consisting of a form superimposed on a second color. Two stimulus control tests followed discrimination training: an attention test in which the form and colors used in training were presented singly and in combination, and then a resistance-to-reinforcement test using the form element of S+ or S- and a novel form. In the attention test, the birds trained with a compound S+ responded most to the S+ compound, less to the S+ color alone, and still less to the S+ form on a dark key. Few responses were made to the negative stimulus, either alone or with the S+ form added. The birds trained with a compound S- pecked most at the S+ color and to a compound of the S+ color with the S- form added. The resistance-to-reinforcement test showed that the birds trained with a compound S+ responded more to the S+ form than to a novel form. However, the birds trained with a compound S- did not reliably respond more to a novel form than to the S- form. These findings suggested that the form element of a compound S+ gains some excitatory control, but the form element of a compound S- does not acquire inhibitory control. The possibility existed that low levels of responding to the S+ form on a dark background in the first experiment were due to use of a darkened key to separate S+ and S- periods during discrimination training. However, the essential findings were the same in a second experiment in which darkening of the chamber separated S+ and S- periods.", "contents": "Attention in the pigeon: testing for excitatory and inhibitory control by the weak elements. In two experiments, pigeons were trained on a successive discrimination between a color and either a compound S+ or a compound S- consisting of a form superimposed on a second color. Two stimulus control tests followed discrimination training: an attention test in which the form and colors used in training were presented singly and in combination, and then a resistance-to-reinforcement test using the form element of S+ or S- and a novel form. In the attention test, the birds trained with a compound S+ responded most to the S+ compound, less to the S+ color alone, and still less to the S+ form on a dark key. Few responses were made to the negative stimulus, either alone or with the S+ form added. The birds trained with a compound S- pecked most at the S+ color and to a compound of the S+ color with the S- form added. The resistance-to-reinforcement test showed that the birds trained with a compound S+ responded more to the S+ form than to a novel form. However, the birds trained with a compound S- did not reliably respond more to a novel form than to the S- form. These findings suggested that the form element of a compound S+ gains some excitatory control, but the form element of a compound S- does not acquire inhibitory control. The possibility existed that low levels of responding to the S+ form on a dark background in the first experiment were due to use of a darkened key to separate S+ and S- periods during discrimination training. However, the essential findings were the same in a second experiment in which darkening of the chamber separated S+ and S- periods."}
{"id": "PMID:469466", "title": "Reference materials and reference methods in clinical chemistry.", "content": "In clinical chemistry today there are often several different methods and instruments in use for the analysis of a particular constituent. When these different methods and instruments are used for careful analysis of the same control specimen, as in assigned value determinations, the assigned values obtained may be significantly different, and these differences may be of clinical importance. In order to determine the reasons for such differences, reference systems or reference points are needed. The reference systems for calibration (calibration materials, standard reference materials) and control (control materials), where the matrices of these different materials must have quite different characteristics, are referred to jointly as reference materials. In order to compare methods, reference methods are needed with known, high reliability. Because of the amount of specimen material needed, the time needed for analysis and the facilities required, reference methods are not suitable for routine analysis. IDeally, standard reference materials are developed first, followed by a definitive method, which is then used to evaluate a candidate reference method. This is currently the case for only a small number of the constituents analyzed in the clinical chemistry laboratory. In this paper the characteristics of the different kinds of reference materials and of reference methods are described. The differences in terminological usage found in the literature are discussed. Limitations of knowledge and technique may necessitate certain compromises, with respect to the ideal characteristics of reference materials and reference methods. Possible compromises are discussed, and the various sources of error associated with them are pointed out. However, these compromises can also lead to improvement in the reliability and comparability of analytical results from different laboratories. As an example of such improvement, the results are presented for assigned value determinations by highly qualified reference laboratories on control specimens for interlaboratory surveys; the decision limits are included and the results are compared with those of the survey participants. Thus various ways are indicated for the use of reference materials and reference methods to improve the reliability and comparability of analytical results, suited to the current state of the art.", "contents": "Reference materials and reference methods in clinical chemistry. In clinical chemistry today there are often several different methods and instruments in use for the analysis of a particular constituent. When these different methods and instruments are used for careful analysis of the same control specimen, as in assigned value determinations, the assigned values obtained may be significantly different, and these differences may be of clinical importance. In order to determine the reasons for such differences, reference systems or reference points are needed. The reference systems for calibration (calibration materials, standard reference materials) and control (control materials), where the matrices of these different materials must have quite different characteristics, are referred to jointly as reference materials. In order to compare methods, reference methods are needed with known, high reliability. Because of the amount of specimen material needed, the time needed for analysis and the facilities required, reference methods are not suitable for routine analysis. IDeally, standard reference materials are developed first, followed by a definitive method, which is then used to evaluate a candidate reference method. This is currently the case for only a small number of the constituents analyzed in the clinical chemistry laboratory. In this paper the characteristics of the different kinds of reference materials and of reference methods are described. The differences in terminological usage found in the literature are discussed. Limitations of knowledge and technique may necessitate certain compromises, with respect to the ideal characteristics of reference materials and reference methods. Possible compromises are discussed, and the various sources of error associated with them are pointed out. However, these compromises can also lead to improvement in the reliability and comparability of analytical results from different laboratories. As an example of such improvement, the results are presented for assigned value determinations by highly qualified reference laboratories on control specimens for interlaboratory surveys; the decision limits are included and the results are compared with those of the survey participants. Thus various ways are indicated for the use of reference materials and reference methods to improve the reliability and comparability of analytical results, suited to the current state of the art."}
{"id": "PMID:469467", "title": "A spectrometric version of the total bilirubin determination with the Du Pont ACA with respect to neonatal sera.", "content": "A spectrometric modification of the determination of total bilirubin with the Du Pont ACA is described. Accuracy, precision and standardization problems are discussed in comparison with the earlier published diazo modification, as well as other techniques well known in the field of neonatal chemistry.", "contents": "A spectrometric version of the total bilirubin determination with the Du Pont ACA with respect to neonatal sera. A spectrometric modification of the determination of total bilirubin with the Du Pont ACA is described. Accuracy, precision and standardization problems are discussed in comparison with the earlier published diazo modification, as well as other techniques well known in the field of neonatal chemistry."}
{"id": "PMID:469468", "title": "Determination of oxalate in urine using oxalate oxidase: comparison with oxalate decarboxylase.", "content": "The oxalate content of urine is determined by means of oxalate oxidase and simple pH measurement. The enzyme specifically decarboxylates oxalate, producing two moles CO2 per mole oxalate. The CO2 diffuses into an alkaline buffer solution (Hallson, P. C. & Rose, G. A. (1974), Clin. Chim. Acta 55, 29--39) in the closed reaction vessel, and reduces the pH value, which is measured with an electrode. Only 125 microliter native urine is required to measure oxalate concentrations in the range of 80 mumol/l to 1.6 mmol/l (corresponding to 7 to 144 mg anhydrous oxalic acid per liter). The limit of detection is 10 nmol oxalate, and the accuracy is 101% with a coefficient of variation of 6%. The method described is insensitive to various interfering factors, such as reducing and oxidizing substances, cloudy or colored samples. It is therefore also suitable for oxalate determination in food technology and plant breeding.", "contents": "Determination of oxalate in urine using oxalate oxidase: comparison with oxalate decarboxylase. The oxalate content of urine is determined by means of oxalate oxidase and simple pH measurement. The enzyme specifically decarboxylates oxalate, producing two moles CO2 per mole oxalate. The CO2 diffuses into an alkaline buffer solution (Hallson, P. C. & Rose, G. A. (1974), Clin. Chim. Acta 55, 29--39) in the closed reaction vessel, and reduces the pH value, which is measured with an electrode. Only 125 microliter native urine is required to measure oxalate concentrations in the range of 80 mumol/l to 1.6 mmol/l (corresponding to 7 to 144 mg anhydrous oxalic acid per liter). The limit of detection is 10 nmol oxalate, and the accuracy is 101% with a coefficient of variation of 6%. The method described is insensitive to various interfering factors, such as reducing and oxidizing substances, cloudy or colored samples. It is therefore also suitable for oxalate determination in food technology and plant breeding."}
{"id": "PMID:469469", "title": "Esterolytic activity of blood serum in infants with hypertrophic pyloric stenosis.", "content": "Esterolytic activity of blood serum was determined in infants with hypertrophic pyloric stenosis and in healthy control children. The substrates hydrolyzed mainly by arylesterase (EC. 3.1.1.2), i.e. p-nitrophenyl esters of acetic, propionic and butyric acid were used. It was found that in infants with hypertrophic pyloric stenosis the rate of p-nitrophenyl propionate hydrolysis was significantly higher than that of the acetate or butyrate. In control group children all three substrates were hydrolysed at similar rates, and these were significantly lower than in infants with hypertrophic pyloric stenosis.", "contents": "Esterolytic activity of blood serum in infants with hypertrophic pyloric stenosis. Esterolytic activity of blood serum was determined in infants with hypertrophic pyloric stenosis and in healthy control children. The substrates hydrolyzed mainly by arylesterase (EC. 3.1.1.2), i.e. p-nitrophenyl esters of acetic, propionic and butyric acid were used. It was found that in infants with hypertrophic pyloric stenosis the rate of p-nitrophenyl propionate hydrolysis was significantly higher than that of the acetate or butyrate. In control group children all three substrates were hydrolysed at similar rates, and these were significantly lower than in infants with hypertrophic pyloric stenosis."}
{"id": "PMID:469470", "title": "Glucose-6-phosphate dehydrogenase deficiency: biochemical and histochemical studies on hair roots for carrier detection.", "content": "Kinetic properties of human hair root glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were studied in order to optimize the assay of these enzymes in lysates from single hair roots. In contrast to previously reported methods, an excess of purified 6-phosphogluconate dehydrogenase was added to the glucose-6-phosphate dehydrogenase reaction mixtures, thus allowing a more exact quantification of glucose-6-phosphate dehydrogenase activity. Although enzyme histochemical techniques suggest a similar distribution of hair root glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, enzyme assays on hair root segments after microdissection nevertheless indicate differences in the distribution of these enzymes. Upon storage a gradual drop in the activity of both hair root enzymes was found, but the rate of decrease in enzyme activity was about equal: the enzyme activity ratio was, therefore, not affected. This opens interesting possibilities for mailing hair roots for screening purposes without any special precautions.", "contents": "Glucose-6-phosphate dehydrogenase deficiency: biochemical and histochemical studies on hair roots for carrier detection. Kinetic properties of human hair root glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were studied in order to optimize the assay of these enzymes in lysates from single hair roots. In contrast to previously reported methods, an excess of purified 6-phosphogluconate dehydrogenase was added to the glucose-6-phosphate dehydrogenase reaction mixtures, thus allowing a more exact quantification of glucose-6-phosphate dehydrogenase activity. Although enzyme histochemical techniques suggest a similar distribution of hair root glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, enzyme assays on hair root segments after microdissection nevertheless indicate differences in the distribution of these enzymes. Upon storage a gradual drop in the activity of both hair root enzymes was found, but the rate of decrease in enzyme activity was about equal: the enzyme activity ratio was, therefore, not affected. This opens interesting possibilities for mailing hair roots for screening purposes without any special precautions."}
{"id": "PMID:469471", "title": "Reduction of sample volume in the SMA 12/60.", "content": "A modification of the flow system of the Technicon SMA 12/60 Continuous Flow Analyzer is described. This modification results in a 60% reduction of the sample volume. The sample size required for the analysis of twelve constituents in serum is thereby reduced to only 0.7 ml. This modification is simple and does not require complex hardware. The reduction in sample size also leads to a considerable cost reduction (25%).", "contents": "Reduction of sample volume in the SMA 12/60. A modification of the flow system of the Technicon SMA 12/60 Continuous Flow Analyzer is described. This modification results in a 60% reduction of the sample volume. The sample size required for the analysis of twelve constituents in serum is thereby reduced to only 0.7 ml. This modification is simple and does not require complex hardware. The reduction in sample size also leads to a considerable cost reduction (25%)."}
{"id": "PMID:469473", "title": "Aldehyde dehydrogenase of the Mongolian gerbil, Meriones unguiculatus.", "content": "The aldehyde dehydrogenase (Aldehyde:NAD(P) oxidoreductase E.C. 1.2.1.3. and 1.2.1.5) phenotype in several tissues of the Mongolian gerbil, Meriones unguiculatus, has been established. The tissue distribution of gerbil aldehyde dehydrogenase is similar to that of the rat, with liver possessing the majority of the aldehyde dehydrognease activity. Male kidney and testis possess significantly more activity than female kidney and ovary. The substrate and co-enzyme specificity of gerbil liver aldehyde dehydrogenase is also similar to that of rat and mouse liver. Gel isoelectric focusing resolves one major gerbil liver aldehyde dehydrogenase isozyme at pI 5.3. Mouse liver is resolved into two major isozymes at pIs 5.3 and 5.6 and rat liver aldehyde dehydrogenase into one major isozyme at pI 5.4. Gerbil liver aldehyde dehydrogenase is functional over a broad pH range with an optima at pH 9.0. Rat and mouse liver aldehyde dehydrogenase possess sharp pH optima at pH 8.5.", "contents": "Aldehyde dehydrogenase of the Mongolian gerbil, Meriones unguiculatus. The aldehyde dehydrogenase (Aldehyde:NAD(P) oxidoreductase E.C. 1.2.1.3. and 1.2.1.5) phenotype in several tissues of the Mongolian gerbil, Meriones unguiculatus, has been established. The tissue distribution of gerbil aldehyde dehydrogenase is similar to that of the rat, with liver possessing the majority of the aldehyde dehydrognease activity. Male kidney and testis possess significantly more activity than female kidney and ovary. The substrate and co-enzyme specificity of gerbil liver aldehyde dehydrogenase is also similar to that of rat and mouse liver. Gel isoelectric focusing resolves one major gerbil liver aldehyde dehydrogenase isozyme at pI 5.3. Mouse liver is resolved into two major isozymes at pIs 5.3 and 5.6 and rat liver aldehyde dehydrogenase into one major isozyme at pI 5.4. Gerbil liver aldehyde dehydrogenase is functional over a broad pH range with an optima at pH 9.0. Rat and mouse liver aldehyde dehydrogenase possess sharp pH optima at pH 8.5."}
{"id": "PMID:469475", "title": "Developmental changes in the pyruvate kinase isozymes of coho salmon.", "content": "Pyruvate kinase exists as two major isozymes in coho salmon. As in mammals and birds, one form is present in the early embryo and maintains a wide tissue distribution in adults. This salmonid type K shows anodal migration during electrophoresis at pH 7.5. The appearence of functional musculature in the developing embryos. In adult animals this second form is the only pyruvate kinase in muscle. Brain, kidney, liver and gill contain primarily the type K pyruvate kinase while heart contains both major forms along with three intermediate forms which presumably constitute a hybrid set. Since there is no additional isozyme restricted to gluconeogenic tissues, we conclude that a type L isozyme has not developed in these animals. The two major isozymes are immunologically distincy. Both forms are dubject to fructose 1,6-bisphosphate activation of phosphoenolpyruvate binding, but the magnitude of the effect is small. The affinities for phosphoenolpyruvate are similar, but salmon type K has hyperbolic saturation curves with this substrate and type M has sigmoidal saturation curves. While the immunological data indicates considerable divergence in structure, the kinetic parameters of the two forms have remained relatively similar.", "contents": "Developmental changes in the pyruvate kinase isozymes of coho salmon. Pyruvate kinase exists as two major isozymes in coho salmon. As in mammals and birds, one form is present in the early embryo and maintains a wide tissue distribution in adults. This salmonid type K shows anodal migration during electrophoresis at pH 7.5. The appearence of functional musculature in the developing embryos. In adult animals this second form is the only pyruvate kinase in muscle. Brain, kidney, liver and gill contain primarily the type K pyruvate kinase while heart contains both major forms along with three intermediate forms which presumably constitute a hybrid set. Since there is no additional isozyme restricted to gluconeogenic tissues, we conclude that a type L isozyme has not developed in these animals. The two major isozymes are immunologically distincy. Both forms are dubject to fructose 1,6-bisphosphate activation of phosphoenolpyruvate binding, but the magnitude of the effect is small. The affinities for phosphoenolpyruvate are similar, but salmon type K has hyperbolic saturation curves with this substrate and type M has sigmoidal saturation curves. While the immunological data indicates considerable divergence in structure, the kinetic parameters of the two forms have remained relatively similar."}
{"id": "PMID:469476", "title": "Stomatogenesis during sexual and asexual reproduction in an amicronucleate strain of Paramecium caudatum.", "content": "Amicronucleate cells of Paramecium caudatum, whose micronuclei have been artifically removed by micropipetting, are characterized by the appearance of a deciliated area at the posterior part of the buccal opening. These cells form food vacuoles at a slightly lower rate than micronucleate cells. Their mean interfission time is longer than that in micronucleates. The exconjugants of amicronucleate cells can not form food vacuoles and eventually die witout fission, though conjugation proceeds normally in them as well as in their micronucleate mate. The oral apparatus of amicronucleate exconjugants seems to be shallower than that of micronucleates. The membranellar cilia, therefore, can be seen through the buccal overture by scanning electron microscope. The results obtained from the cross of micronucleate and amicronucleate strains and from the induction of autogamy in amicronucleate strains suggest that the micronucleus has a primary role in developing the normal oral apparatus after nuclear reorganization.", "contents": "Stomatogenesis during sexual and asexual reproduction in an amicronucleate strain of Paramecium caudatum. Amicronucleate cells of Paramecium caudatum, whose micronuclei have been artifically removed by micropipetting, are characterized by the appearance of a deciliated area at the posterior part of the buccal opening. These cells form food vacuoles at a slightly lower rate than micronucleate cells. Their mean interfission time is longer than that in micronucleates. The exconjugants of amicronucleate cells can not form food vacuoles and eventually die witout fission, though conjugation proceeds normally in them as well as in their micronucleate mate. The oral apparatus of amicronucleate exconjugants seems to be shallower than that of micronucleates. The membranellar cilia, therefore, can be seen through the buccal overture by scanning electron microscope. The results obtained from the cross of micronucleate and amicronucleate strains and from the induction of autogamy in amicronucleate strains suggest that the micronucleus has a primary role in developing the normal oral apparatus after nuclear reorganization."}
{"id": "PMID:469477", "title": "HCO3-stimulated Cl efflux in the gulf toadfish acclimated to sea water.", "content": "Unidirectional efflux of Cl was examined in the Gulf toadfish, Opsanus beta, in artificial seawater solutions with modified concentrations of Cl and HCO3. Removal of Cl HCO3 reduced Cl efflux. Addition of HCO3 at typical seawater concentrations stimulated Cl efflux, independent of changes in the transepithelial potential. This active, HCO3-stimulated Cl efflux is saturable, with a Km of 2.4 mM, typical of the concentration of HCO3 found in sea water, and independent of external pH. Active extrusion of Cl offsets the net diffusional and oral gain of Cl faced by O. beta in sea water.", "contents": "HCO3-stimulated Cl efflux in the gulf toadfish acclimated to sea water. Unidirectional efflux of Cl was examined in the Gulf toadfish, Opsanus beta, in artificial seawater solutions with modified concentrations of Cl and HCO3. Removal of Cl HCO3 reduced Cl efflux. Addition of HCO3 at typical seawater concentrations stimulated Cl efflux, independent of changes in the transepithelial potential. This active, HCO3-stimulated Cl efflux is saturable, with a Km of 2.4 mM, typical of the concentration of HCO3 found in sea water, and independent of external pH. Active extrusion of Cl offsets the net diffusional and oral gain of Cl faced by O. beta in sea water."}
{"id": "PMID:469478", "title": "In vitro development of core cells of the inner cell mass of the mouse blastocyst: effects of conditioned medium.", "content": "Blastocysts submitted to two rounds of immunosurgery give rise to cores of presumptive ectoderm cells, many of which do not survive for more than 48 hours when cultured individually. Precoating of the culture plates with conditioned medium (CM) from PYS-2 cells increases the incidence with which cores regenerate an outer layer. This procedure also improves the survival frequency of the cores, but only for a limited period of time. The small number of cores which survive for two weeks or more, either in uncoated or CM-coated plates, give rise to any array of cell types, including giant cells resembling trophoblast.", "contents": "In vitro development of core cells of the inner cell mass of the mouse blastocyst: effects of conditioned medium. Blastocysts submitted to two rounds of immunosurgery give rise to cores of presumptive ectoderm cells, many of which do not survive for more than 48 hours when cultured individually. Precoating of the culture plates with conditioned medium (CM) from PYS-2 cells increases the incidence with which cores regenerate an outer layer. This procedure also improves the survival frequency of the cores, but only for a limited period of time. The small number of cores which survive for two weeks or more, either in uncoated or CM-coated plates, give rise to any array of cell types, including giant cells resembling trophoblast."}
{"id": "PMID:469479", "title": "In vitro synthesis of RNA by Xenopus spermatogenic cells I. Evidence for polyadenylated and non-polyadenylated RNA synthesis in different cell populations.", "content": "Premeiotic and postmeiotic (haploid) gene expression during spermatogenesis in the anuran, Xenopus laevis, was studied by analyzing the accumulation of radioactively labelled cytoplasmic polyadenylated [poly (A +)] and non-polyadenylated [poly (A -)] RNAs. Dissociated spermatogenic cells were labelled and maintained in an in vitro system capable of supporting cell differentiation. Labelled cells were separated by density gradient centrifugation into subpopulations enriched for individual spermatogenic stages. RNA was extracted and purified from each cell fraction, and separated into poly (A +) and poly (A -) species. Comparison of poly (A +) to non-poly (A) radioactivity in cells labelled with tritiated uridine or adenosine demonstrated that (1) all cell fractions produced significant quantities of polyadenylated RNA relative to total RNA synthesis; and (2) that a cell fraction enriched for pachytene spermatocyte RNA contained up to 15% of total cytoplasmic and 35% of total polysomal RNA labelled as poly (A +) containing species. RNA was also characterized by sucrose density gradient centrifugation and polyacrylamide gel electrophoresis. All cell types showed typical poly (A -) peaks of 4S, 18S and 28S, corresponding to tRNA (4S) and rRNAs (18, 28S) respectively. Spermatids and spermatozoa had additional absorbance peaks at 13 and 21S which cosedimented with Xenopus oocyte mitochondrial rRNA. Patterns of incorporation of uridine and adenosine into poly (A +) RNA in all germ cell fractions tested were complex. In all cases, major areas of radioactivity were found in a broad band sedimenting between 6-17S. Spermatid fractions showed a prominent peak of incorporation at 6-8S, while pachytene cells also showed heavier poly (A +) peaks in the 17-25S region. A non-polyadenylated RNA species sedimenting at 6-8S with a relatively rapid rate of turnover was also observed in spermatids. From these results it is concluded that synthesis of transfer, ribosomal, and putative messenger RNA species continues in spermatogenic cells throughout all but the very last stages of spermatogenesis in Xenopus.", "contents": "In vitro synthesis of RNA by Xenopus spermatogenic cells I. Evidence for polyadenylated and non-polyadenylated RNA synthesis in different cell populations. Premeiotic and postmeiotic (haploid) gene expression during spermatogenesis in the anuran, Xenopus laevis, was studied by analyzing the accumulation of radioactively labelled cytoplasmic polyadenylated [poly (A +)] and non-polyadenylated [poly (A -)] RNAs. Dissociated spermatogenic cells were labelled and maintained in an in vitro system capable of supporting cell differentiation. Labelled cells were separated by density gradient centrifugation into subpopulations enriched for individual spermatogenic stages. RNA was extracted and purified from each cell fraction, and separated into poly (A +) and poly (A -) species. Comparison of poly (A +) to non-poly (A) radioactivity in cells labelled with tritiated uridine or adenosine demonstrated that (1) all cell fractions produced significant quantities of polyadenylated RNA relative to total RNA synthesis; and (2) that a cell fraction enriched for pachytene spermatocyte RNA contained up to 15% of total cytoplasmic and 35% of total polysomal RNA labelled as poly (A +) containing species. RNA was also characterized by sucrose density gradient centrifugation and polyacrylamide gel electrophoresis. All cell types showed typical poly (A -) peaks of 4S, 18S and 28S, corresponding to tRNA (4S) and rRNAs (18, 28S) respectively. Spermatids and spermatozoa had additional absorbance peaks at 13 and 21S which cosedimented with Xenopus oocyte mitochondrial rRNA. Patterns of incorporation of uridine and adenosine into poly (A +) RNA in all germ cell fractions tested were complex. In all cases, major areas of radioactivity were found in a broad band sedimenting between 6-17S. Spermatid fractions showed a prominent peak of incorporation at 6-8S, while pachytene cells also showed heavier poly (A +) peaks in the 17-25S region. A non-polyadenylated RNA species sedimenting at 6-8S with a relatively rapid rate of turnover was also observed in spermatids. From these results it is concluded that synthesis of transfer, ribosomal, and putative messenger RNA species continues in spermatogenic cells throughout all but the very last stages of spermatogenesis in Xenopus."}
{"id": "PMID:469480", "title": "Solute compatibility with enzyme function and structure: rationales for the selection of osmotic agents and end-products of anaerobic metabolism in marine invertebrates.", "content": "The major nitrogenous osmolytes present in the cells of marine invertebrates, notably the free amino acids glycine, alanine and proline, and trimethylamine oxide and betaine, are highly compatible with proper enzyme function and structure. These nitrogenous osmolytes display either non-perturbing or, in some cases, favorable effects on enzyme-substrate and enzyme-cofactor complex formation, catalytic velocity and protein structural stability. In contrast, inorganic salts (KCl and NaCl) and certain of the free amino acids which play only a minor osmotic role, e.g., arginine and lysine, have strongly perturbing effects on one or more of these enzymic parameters. The compatible nitrogenous solutes therefore are suitable for use at high (several tenths molar) concentrations and at widely varying concentrations in osmo-conforming species. Certain nitrogenous solutes, especially trimethylamine oxide, betaine and glutamate, offset some of the perturbing effects of inorganic ions on enzyme function. The selective accumulation of osmolytes thus involves not only the concentration of non-perturbing solutes, but also a balanced accumulation of solutes with opposing effects on enzymes. The selection of end-products of anaerobic metabolism also appears to be based, in part, on considerations of solute compatibility with enzyme function. Octopine is a non-perturbing solute, whereas arginine, which is condensed with pyruvate to form octopine, is very strongly perturbing. Succinate has marked stabilizing effects on protein structure. We conclude that the composition of the intracellular fluids of marine invertebrates reflects selection for osmolytes and end-products whose net effects create a cellular microenvironment which is conducive to optimal enzyme function and structure. The accumulation of compatible solutes may preclude the necessity for widespread changes in protein structure in adapting to concentrated or highly variable osmotic environments.", "contents": "Solute compatibility with enzyme function and structure: rationales for the selection of osmotic agents and end-products of anaerobic metabolism in marine invertebrates. The major nitrogenous osmolytes present in the cells of marine invertebrates, notably the free amino acids glycine, alanine and proline, and trimethylamine oxide and betaine, are highly compatible with proper enzyme function and structure. These nitrogenous osmolytes display either non-perturbing or, in some cases, favorable effects on enzyme-substrate and enzyme-cofactor complex formation, catalytic velocity and protein structural stability. In contrast, inorganic salts (KCl and NaCl) and certain of the free amino acids which play only a minor osmotic role, e.g., arginine and lysine, have strongly perturbing effects on one or more of these enzymic parameters. The compatible nitrogenous solutes therefore are suitable for use at high (several tenths molar) concentrations and at widely varying concentrations in osmo-conforming species. Certain nitrogenous solutes, especially trimethylamine oxide, betaine and glutamate, offset some of the perturbing effects of inorganic ions on enzyme function. The selective accumulation of osmolytes thus involves not only the concentration of non-perturbing solutes, but also a balanced accumulation of solutes with opposing effects on enzymes. The selection of end-products of anaerobic metabolism also appears to be based, in part, on considerations of solute compatibility with enzyme function. Octopine is a non-perturbing solute, whereas arginine, which is condensed with pyruvate to form octopine, is very strongly perturbing. Succinate has marked stabilizing effects on protein structure. We conclude that the composition of the intracellular fluids of marine invertebrates reflects selection for osmolytes and end-products whose net effects create a cellular microenvironment which is conducive to optimal enzyme function and structure. The accumulation of compatible solutes may preclude the necessity for widespread changes in protein structure in adapting to concentrated or highly variable osmotic environments."}
{"id": "PMID:469481", "title": "Distribution of phenylalanine hydroxylase (EC 1.14.3.1) in liver and kidney of vertebrates.", "content": "The range of phenylalanine hydroxylase activity was determined by measuring the conversion of radioactive phenylalanine to tyrosine in liver and kidney of various vertebrates. Rodents (rats, mouse, gerbil, hamster and guinea pig) were found to have the highest liver phenylalanine hydroxylase activity among all animals studied. They are also the only species that possessed a significant kidney phenylalanine hydroxylase activity which was about 25% of that found in the liver of the same animal. The synthetic dimethyl-tetrahydro-pteridine, used as a cofactor for the enzyme assay in most studies, catalyzed non-enzymatic hydroxylation of phenylalanine to tyrosine. Inclusion of boiled-blank and strict control of timing between incubation and product measurement were essential precautions to minimize erroneous results from substrate contamination and non-enzymatic hydroxylation.", "contents": "Distribution of phenylalanine hydroxylase (EC 1.14.3.1) in liver and kidney of vertebrates. The range of phenylalanine hydroxylase activity was determined by measuring the conversion of radioactive phenylalanine to tyrosine in liver and kidney of various vertebrates. Rodents (rats, mouse, gerbil, hamster and guinea pig) were found to have the highest liver phenylalanine hydroxylase activity among all animals studied. They are also the only species that possessed a significant kidney phenylalanine hydroxylase activity which was about 25% of that found in the liver of the same animal. The synthetic dimethyl-tetrahydro-pteridine, used as a cofactor for the enzyme assay in most studies, catalyzed non-enzymatic hydroxylation of phenylalanine to tyrosine. Inclusion of boiled-blank and strict control of timing between incubation and product measurement were essential precautions to minimize erroneous results from substrate contamination and non-enzymatic hydroxylation."}
{"id": "PMID:469482", "title": "Autoradiographic and electron microscopic studies of minced cardiac muscle regeneration in the adult newt, notophthalmus viridescens.", "content": "The regenerative response of minced cardiac muscle grafts in the adult newt was studied using autoradiography and electron microscopy. One-sixteenth to one-eighth of the newt ventricle was amputated, minced, and returned to the wounded ventricle. At five days after grafting, no reorganization of graft msucle pieces was apparent and there was degeneration of much of the muscle graft. Another, smaller population of 5-day myocytes had euchromatic nuclei and intact sarcolemmae. In 10- and 16-day grafts, continuity between ventricular and graft lumina was established and coalescence of graft pieces was apparent. Ultrastructurally, 10- and 16-day graft myocytes appeared to have fewer myofibrillae and increased amounts of rough endoplasmic reticulum, polyribosomes, Golig complexes, and dense bodies when compared to uninjured ventricular myocytes. The peak of proliferative activity of graft cells was observed at 16 days. Electron microscopic autoradiography revealed breadkdown of myofibrillar structure in labeled myocytes, whereas in myocytes in the later stages of mitosis only scattered myofilaments and no Z bands were present. By 30 days, grafts appeared as an integrated structure composed primarily of cardiac muscle. Myocytes of 30-day grafts were observed in various stages of myofibrillogenesis and contained numberous 10-nm filaments. Seventy-day graft mycoytes had numberous well organized myofibrillae and intercellular junctions similar to those seen in uninjured ventricular myocytes.", "contents": "Autoradiographic and electron microscopic studies of minced cardiac muscle regeneration in the adult newt, notophthalmus viridescens. The regenerative response of minced cardiac muscle grafts in the adult newt was studied using autoradiography and electron microscopy. One-sixteenth to one-eighth of the newt ventricle was amputated, minced, and returned to the wounded ventricle. At five days after grafting, no reorganization of graft msucle pieces was apparent and there was degeneration of much of the muscle graft. Another, smaller population of 5-day myocytes had euchromatic nuclei and intact sarcolemmae. In 10- and 16-day grafts, continuity between ventricular and graft lumina was established and coalescence of graft pieces was apparent. Ultrastructurally, 10- and 16-day graft myocytes appeared to have fewer myofibrillae and increased amounts of rough endoplasmic reticulum, polyribosomes, Golig complexes, and dense bodies when compared to uninjured ventricular myocytes. The peak of proliferative activity of graft cells was observed at 16 days. Electron microscopic autoradiography revealed breadkdown of myofibrillar structure in labeled myocytes, whereas in myocytes in the later stages of mitosis only scattered myofilaments and no Z bands were present. By 30 days, grafts appeared as an integrated structure composed primarily of cardiac muscle. Myocytes of 30-day grafts were observed in various stages of myofibrillogenesis and contained numberous 10-nm filaments. Seventy-day graft mycoytes had numberous well organized myofibrillae and intercellular junctions similar to those seen in uninjured ventricular myocytes."}
{"id": "PMID:469483", "title": "Abnormal scale morphogenesis: relationship of polypeptide patterns to action of the scaleless gene.", "content": "The polyacrylamide gradient gel electrophoresis (PAGGE) pattern of polypeptides isolated from normal scuttate scale epidermis of 1-week-old chicks was different from that of the anterior shank epidermis from 1-week-old scaleless mutant chicks. The PAGGE patterns of polypeptides isolated from normal and scaleless reticulate scale epidermis (from 1-week-old chicks) differed by only one band, whereas comparison of mutant's scuttate and reticulate patterns showed three band differences. These data are discussed in relation to the action of the scaleless gene on early morphogenesis of the two types of scales.", "contents": "Abnormal scale morphogenesis: relationship of polypeptide patterns to action of the scaleless gene. The polyacrylamide gradient gel electrophoresis (PAGGE) pattern of polypeptides isolated from normal scuttate scale epidermis of 1-week-old chicks was different from that of the anterior shank epidermis from 1-week-old scaleless mutant chicks. The PAGGE patterns of polypeptides isolated from normal and scaleless reticulate scale epidermis (from 1-week-old chicks) differed by only one band, whereas comparison of mutant's scuttate and reticulate patterns showed three band differences. These data are discussed in relation to the action of the scaleless gene on early morphogenesis of the two types of scales."}
{"id": "PMID:469484", "title": "Effects of accelerating stimulation on different indices of development in Japanese quail embryos.", "content": "Effects of continuous accelerating stimulation on the timing, duration and rate of occurrence of different indices of development in Japanese quail embryos were examined. Indices used were: the onset of breathing and hatching, also pipping, clicking, vocalisation, membrane penetration, yolk sac withdrawal and lung aeration. Results showed that embryos stimulated by clicks began breathing about nine hours in advance of unstimulated controls and hatched about 23 hours in advance. All other indices occurred early in comparison with controls but not all occurred at the same accelerated rate and some having begun early, proceeded at the same rate as controls then were completed rapidly just before hatching. Developmental problems are discussed in the light of these results and it is suggested that continuous accelerating stimulation affects development in two main stages.", "contents": "Effects of accelerating stimulation on different indices of development in Japanese quail embryos. Effects of continuous accelerating stimulation on the timing, duration and rate of occurrence of different indices of development in Japanese quail embryos were examined. Indices used were: the onset of breathing and hatching, also pipping, clicking, vocalisation, membrane penetration, yolk sac withdrawal and lung aeration. Results showed that embryos stimulated by clicks began breathing about nine hours in advance of unstimulated controls and hatched about 23 hours in advance. All other indices occurred early in comparison with controls but not all occurred at the same accelerated rate and some having begun early, proceeded at the same rate as controls then were completed rapidly just before hatching. Developmental problems are discussed in the light of these results and it is suggested that continuous accelerating stimulation affects development in two main stages."}
{"id": "PMID:469485", "title": "Epidermal-dermal recombinations with embryonic naked and normal back skin of Gallus domesticus.", "content": "Birds exhibiting a varying featherless condition resulting from the recessive sex-linked gene naked (n) were used to investigate whether the gene altered the dermis or the epidermis. By splitting 7-day normal and naked skin into its dermal and epidermal components, and heterotypically recombining and growing it in chambers on the chorio-allantoic membrane (CAM), it was found that the epidermis of the naked birds is the site of mutant gene action. A histological study of developing normal and naked skin was done and the structure of the naked feather is elucidated.", "contents": "Epidermal-dermal recombinations with embryonic naked and normal back skin of Gallus domesticus. Birds exhibiting a varying featherless condition resulting from the recessive sex-linked gene naked (n) were used to investigate whether the gene altered the dermis or the epidermis. By splitting 7-day normal and naked skin into its dermal and epidermal components, and heterotypically recombining and growing it in chambers on the chorio-allantoic membrane (CAM), it was found that the epidermis of the naked birds is the site of mutant gene action. A histological study of developing normal and naked skin was done and the structure of the naked feather is elucidated."}
{"id": "PMID:469486", "title": "Intermediate filaments connect z-discs in adult chicken muscle.", "content": "When adult chicken skeletal myofibrils are treated with a myosin-extracting solution, the Z-discs with attached actin filaments retain their linear connections with one another in the extracted myofibril. The sarcomere length increases in the extracted myofibrils from a control lenght of 2.5 micrometer up to 6 micrometer. In a sarcomere, eight to fifty 10 nm filaments can be seen in parallel array in the H-zone. The 10 nm-wide filaments do not bind heavy meromyosin and are two to four micrometers in length. These intermediate filaments are postulated to be an integral part of the sarcomere, connecting Z-bands along the length of the myofibril.", "contents": "Intermediate filaments connect z-discs in adult chicken muscle. When adult chicken skeletal myofibrils are treated with a myosin-extracting solution, the Z-discs with attached actin filaments retain their linear connections with one another in the extracted myofibril. The sarcomere length increases in the extracted myofibrils from a control lenght of 2.5 micrometer up to 6 micrometer. In a sarcomere, eight to fifty 10 nm filaments can be seen in parallel array in the H-zone. The 10 nm-wide filaments do not bind heavy meromyosin and are two to four micrometers in length. These intermediate filaments are postulated to be an integral part of the sarcomere, connecting Z-bands along the length of the myofibril."}
{"id": "PMID:469503", "title": "Relationships among different stages of Piagetian tasks and spatial relations in adolescents.", "content": "High school students aged 15 through 19 years (N = 116) were administered three Piagetian type tasks (conservation of weight, paper and pencil tests of concrete-operational reasoning and formal-operational reasoning) and a measure of spatial relations (the Revised Minnesota Paper Form Board Test). While a path analysis showed there were logically expected relationships among the tasks, neither sex differences nor age differences were found on any of the measures.", "contents": "Relationships among different stages of Piagetian tasks and spatial relations in adolescents. High school students aged 15 through 19 years (N = 116) were administered three Piagetian type tasks (conservation of weight, paper and pencil tests of concrete-operational reasoning and formal-operational reasoning) and a measure of spatial relations (the Revised Minnesota Paper Form Board Test). While a path analysis showed there were logically expected relationships among the tasks, neither sex differences nor age differences were found on any of the measures."}
{"id": "PMID:469504", "title": "Memorizing and copying visual patterns: a Piagetian interpretation.", "content": "Six-, 8-, 10-, and 12-year-old children (10 boys and 10 girls) reconstructed two visual patterns from immediate memory, while other 5- and 6-year-old children (10 boys and 10 girls) reconstructed the identical patterns by direct copying. Patterns were simple and composed entirely of circles or squares as component items. Four results were emphasized: (a) Numerous errors mady by the copying groups led to the conclusion that memory loss is often overestimated in young children. Since an independent estimate of perceptual encoding errors is rarely carried out, encoding mistakes are often included among forgetting errors. (b) One pattern was both copied and remembered more poorly than the other in accord with a Piagetian interpretation of a conceptual conflict inherent in the pattern design between spatial and numerical correspondence of component pattern items. (c) A memory strategy emphasizing configuration preservation was suggested for the 6-year-olds who made slightly fewer memory than copying errors for two configural scoring categories. (d) Performance in an unrelated planning-for-memory task significantly differentiated between better and worse performers on the visual pattern memory task.", "contents": "Memorizing and copying visual patterns: a Piagetian interpretation. Six-, 8-, 10-, and 12-year-old children (10 boys and 10 girls) reconstructed two visual patterns from immediate memory, while other 5- and 6-year-old children (10 boys and 10 girls) reconstructed the identical patterns by direct copying. Patterns were simple and composed entirely of circles or squares as component items. Four results were emphasized: (a) Numerous errors mady by the copying groups led to the conclusion that memory loss is often overestimated in young children. Since an independent estimate of perceptual encoding errors is rarely carried out, encoding mistakes are often included among forgetting errors. (b) One pattern was both copied and remembered more poorly than the other in accord with a Piagetian interpretation of a conceptual conflict inherent in the pattern design between spatial and numerical correspondence of component pattern items. (c) A memory strategy emphasizing configuration preservation was suggested for the 6-year-olds who made slightly fewer memory than copying errors for two configural scoring categories. (d) Performance in an unrelated planning-for-memory task significantly differentiated between better and worse performers on the visual pattern memory task."}
{"id": "PMID:469505", "title": "Geometric problem solving related to differences in sex and mathematical interests.", "content": "Sex differences in Einstellung Effects were confounded by attitudinal factors. Hypothesizing that this was the case for spatial visualization and restructurization, we compared male and female college students' solutions of three elementary geometric problems. Used by Max Wertheimer to study productive thinking, they call for a Gestalt, spatial approach. In group administration to 86 calculus students, males did somewhat better than females, but the reverse held in classes where most women majored in mathematics. Individual administration to 200 Ss, balanced for sex and major, showed that female mathematics majors had more solutions than other females: e.g., 51 percent more compared to 26 percent sex differences. Four hints were available for restructuring each problem. The percentage needing all four hints was highest for male nonmathematics major and next highest for female mathematics majors (e.g., 75 and 23 percent, respectively). Embarrassment at needing hints in such \"easy problems\" and ego-involvement were factors. Thus, sex differences were less pronounced than differences related to mathematical abilities and interests and to task-and ego-concerns.", "contents": "Geometric problem solving related to differences in sex and mathematical interests. Sex differences in Einstellung Effects were confounded by attitudinal factors. Hypothesizing that this was the case for spatial visualization and restructurization, we compared male and female college students' solutions of three elementary geometric problems. Used by Max Wertheimer to study productive thinking, they call for a Gestalt, spatial approach. In group administration to 86 calculus students, males did somewhat better than females, but the reverse held in classes where most women majored in mathematics. Individual administration to 200 Ss, balanced for sex and major, showed that female mathematics majors had more solutions than other females: e.g., 51 percent more compared to 26 percent sex differences. Four hints were available for restructuring each problem. The percentage needing all four hints was highest for male nonmathematics major and next highest for female mathematics majors (e.g., 75 and 23 percent, respectively). Embarrassment at needing hints in such \"easy problems\" and ego-involvement were factors. Thus, sex differences were less pronounced than differences related to mathematical abilities and interests and to task-and ego-concerns."}
{"id": "PMID:469506", "title": "Sex differences in the association of cerebral hemispheric specialization of spatial function with conservation task performance.", "content": "The association of locus of cerebral hemispheric specialization of spatial function with identity and equivalence conservation judgments was tested in a group of 4- to 6-year-old right-handed children (N = 31). Separate multiple regression analyses for males and females revealed that locus of specialization of spatial function was irrelevant for performance on the equivalence conservation task but was a significant factor in male identity conservation performance. Whereas right hemisphere specialization was advantageous for males, it was of no significant import for females. Results are discussed in the light of previous research on the use of differing cognitive stratergies by males and females in problem-solving situations.", "contents": "Sex differences in the association of cerebral hemispheric specialization of spatial function with conservation task performance. The association of locus of cerebral hemispheric specialization of spatial function with identity and equivalence conservation judgments was tested in a group of 4- to 6-year-old right-handed children (N = 31). Separate multiple regression analyses for males and females revealed that locus of specialization of spatial function was irrelevant for performance on the equivalence conservation task but was a significant factor in male identity conservation performance. Whereas right hemisphere specialization was advantageous for males, it was of no significant import for females. Results are discussed in the light of previous research on the use of differing cognitive stratergies by males and females in problem-solving situations."}
{"id": "PMID:469509", "title": "Neurobiology, culture, and behavior disturbances. An integrative review.", "content": "The study of the influence of culture on behavior disturbances has a long history in psychiatry. Attention has principally focused on etiological questions and on how psychiatric illness is handled socially, although there is a tradition which deals with the manifestations of so-called functional psychiatric illnesses. In all of these instances, the methods and rationale of the social sciences have been employed and underlying neurobiological factors neglected. However, since a people's culture is learned, it is obviously internalized in some way in the individual. A basic question is how cultural influences may possibly affect the organization and functioning of the nervous system and, by extension, behavior and its disturbances. The relevance of this topic for psychiatry and the social sciences is illustrated by analysis of four prototypical organic psychiatric illnesses.", "contents": "Neurobiology, culture, and behavior disturbances. An integrative review. The study of the influence of culture on behavior disturbances has a long history in psychiatry. Attention has principally focused on etiological questions and on how psychiatric illness is handled socially, although there is a tradition which deals with the manifestations of so-called functional psychiatric illnesses. In all of these instances, the methods and rationale of the social sciences have been employed and underlying neurobiological factors neglected. However, since a people's culture is learned, it is obviously internalized in some way in the individual. A basic question is how cultural influences may possibly affect the organization and functioning of the nervous system and, by extension, behavior and its disturbances. The relevance of this topic for psychiatry and the social sciences is illustrated by analysis of four prototypical organic psychiatric illnesses."}
{"id": "PMID:469510", "title": "Why should dialysis benefit schizophrenia?", "content": "If dialysis is successful for the treatment of schizophrenia, the artificial kidney must be removing something which the human kidney cannot, unless other nonspecific factors are involved. Either there is an abnormal compound present which is retained by normal renal tubular transport processes, or the kidney of a schizophrenic must be abnormal in that it fails to excrete a normally present compound. Since the latter explanation is less probable, biochemical research should focus on classes of compounds which are known to be handled differently by the artificial kidney than by the human kidney. A dialyzable \"schizophrenic toxin\" might be a nonprotein, nonprotein-bound of molecular weight below 500 daltons such as an organic acid or base. Proteins, peptides, amino acids, and trace elements are less likely possibilities.", "contents": "Why should dialysis benefit schizophrenia? If dialysis is successful for the treatment of schizophrenia, the artificial kidney must be removing something which the human kidney cannot, unless other nonspecific factors are involved. Either there is an abnormal compound present which is retained by normal renal tubular transport processes, or the kidney of a schizophrenic must be abnormal in that it fails to excrete a normally present compound. Since the latter explanation is less probable, biochemical research should focus on classes of compounds which are known to be handled differently by the artificial kidney than by the human kidney. A dialyzable \"schizophrenic toxin\" might be a nonprotein, nonprotein-bound of molecular weight below 500 daltons such as an organic acid or base. Proteins, peptides, amino acids, and trace elements are less likely possibilities."}
{"id": "PMID:469511", "title": "Premorbid social competence and outcome among schizophrenic and nonschizophrenic patients.", "content": "The relation between premorbid social competence and outcome was examined with 381 male state hospital patients in four diagnostic categories: schizophrenia, affective reaction, psychoneurotic disorder, and personality disorder. Outcome was assessed using the measures of length of initial hospitalization, total length of rehospitalization, and number of readmissions. The follow-up period was 3 years after discharge from the first hospitalization. On all outcome measures, higher social competence was significantly related to favorable outcome. The four diagnostic groups differed significantly in social competence level, but no evidence was found to indicate that the social competence-outcome relation was influenced by diagnosis. Results were interpreted as consistent with a developmental formulation and as indicating that the relation between premorbid social competence and outcome is not unique to schizophrenia but obtains over a broad range of diagnoses.", "contents": "Premorbid social competence and outcome among schizophrenic and nonschizophrenic patients. The relation between premorbid social competence and outcome was examined with 381 male state hospital patients in four diagnostic categories: schizophrenia, affective reaction, psychoneurotic disorder, and personality disorder. Outcome was assessed using the measures of length of initial hospitalization, total length of rehospitalization, and number of readmissions. The follow-up period was 3 years after discharge from the first hospitalization. On all outcome measures, higher social competence was significantly related to favorable outcome. The four diagnostic groups differed significantly in social competence level, but no evidence was found to indicate that the social competence-outcome relation was influenced by diagnosis. Results were interpreted as consistent with a developmental formulation and as indicating that the relation between premorbid social competence and outcome is not unique to schizophrenia but obtains over a broad range of diagnoses."}
{"id": "PMID:469512", "title": "A study of schizophrenics' ability to localize the source of a sound.", "content": "Twelve process, nonparanoid schizophrenics, 12 nonschizophrenic psychiatric controls, and 12 normal controls were given an auditory localization task. Performance was measured by the number of correct localizations and was studied as a function of three variables: diagnosis, position of the apparatus in relation to the subject's median plane (right or left), and the degree of displacement of the auditory stimulus from a fixation point (4.5 degrees, 3.0 degrees, and 1.5 degrees). A three-way analysis of variance with two repeated measures resulted in a significant main effect for each of the three independent variables. Orthogonal comparisons indicated no difference in performance between normal and psychiatric controls but a significant difference between schizophrenics and the combined controls. The degree of displacement of the auditory stimulus resulted in a significant linear trend in performance. There were no significant interactions. The present findings strongly suggest that process, nonparanoid schizophrenics suffer from a specific deficit in auditory localization.", "contents": "A study of schizophrenics' ability to localize the source of a sound. Twelve process, nonparanoid schizophrenics, 12 nonschizophrenic psychiatric controls, and 12 normal controls were given an auditory localization task. Performance was measured by the number of correct localizations and was studied as a function of three variables: diagnosis, position of the apparatus in relation to the subject's median plane (right or left), and the degree of displacement of the auditory stimulus from a fixation point (4.5 degrees, 3.0 degrees, and 1.5 degrees). A three-way analysis of variance with two repeated measures resulted in a significant main effect for each of the three independent variables. Orthogonal comparisons indicated no difference in performance between normal and psychiatric controls but a significant difference between schizophrenics and the combined controls. The degree of displacement of the auditory stimulus resulted in a significant linear trend in performance. There were no significant interactions. The present findings strongly suggest that process, nonparanoid schizophrenics suffer from a specific deficit in auditory localization."}
{"id": "PMID:469513", "title": "The elicitation of visual hallucinations via brief instructions in a normal sample.", "content": "College students tested in groups were given brief suggestions either to \"see\" or to \"imagine\" a kitten. Subjects responded twice, once with eyes open and once with eyes closed. They rated the duration, transparency, and vividness of their imagery, and also their involvement in and belief in the reality of their imaginings under both eye closure conditions. \"Imagine\" suggestions elicited imagery of longer duration than \"see\" suggestions. Although subjects reported longer, more vivid, and less transparent imagery with their eyes closed, this effect was dependent on the order of eye closure (i.e., whether the eyes closed instruction preceded or followed the eyes open instruction). The three dimensions of imagery intercorrelated, and each dimension also correlated with subjects' degree of involvement in and belief in their imaginings. A minority of subjects given both the \"see\" and \"imagine\" suggestions reported believing that their imaginings were real events during the suggestion period. Furthermore, belief in imaginings was the only variable that correlated with hypnotic susceptibility. These data indicate that hallucinations (i.e., believed-in imaginings) can be elicited from a minority of \"norman\" subjects with brief instructions.", "contents": "The elicitation of visual hallucinations via brief instructions in a normal sample. College students tested in groups were given brief suggestions either to \"see\" or to \"imagine\" a kitten. Subjects responded twice, once with eyes open and once with eyes closed. They rated the duration, transparency, and vividness of their imagery, and also their involvement in and belief in the reality of their imaginings under both eye closure conditions. \"Imagine\" suggestions elicited imagery of longer duration than \"see\" suggestions. Although subjects reported longer, more vivid, and less transparent imagery with their eyes closed, this effect was dependent on the order of eye closure (i.e., whether the eyes closed instruction preceded or followed the eyes open instruction). The three dimensions of imagery intercorrelated, and each dimension also correlated with subjects' degree of involvement in and belief in their imaginings. A minority of subjects given both the \"see\" and \"imagine\" suggestions reported believing that their imaginings were real events during the suggestion period. Furthermore, belief in imaginings was the only variable that correlated with hypnotic susceptibility. These data indicate that hallucinations (i.e., believed-in imaginings) can be elicited from a minority of \"norman\" subjects with brief instructions."}
{"id": "PMID:469514", "title": "Mood changes and flooding outcome in obsessive-compulsive patients. Report of a 2-year follow-up.", "content": "Twenty-five chronic obsessive-compulsive patients treated with flooding were investiaged retrospectively after 2 years by an independent assessor for the presence of depressive mood fluctuations during their life histories using a five-point rating scale. Patients themselves also completed rating scales for obsessional phenomena during the pre-, post-treatment, and follow-up assessment. Fifteen patients were characterized as not having exhibited mood changes and 10 as having such changes. Patients without changes responded promptly to flooding treatment and showed further improvement during follow-up. Patients with mood changes, although responding well to initially delivered flooding sessions, showed significant relapse at follow-up.", "contents": "Mood changes and flooding outcome in obsessive-compulsive patients. Report of a 2-year follow-up. Twenty-five chronic obsessive-compulsive patients treated with flooding were investiaged retrospectively after 2 years by an independent assessor for the presence of depressive mood fluctuations during their life histories using a five-point rating scale. Patients themselves also completed rating scales for obsessional phenomena during the pre-, post-treatment, and follow-up assessment. Fifteen patients were characterized as not having exhibited mood changes and 10 as having such changes. Patients without changes responded promptly to flooding treatment and showed further improvement during follow-up. Patients with mood changes, although responding well to initially delivered flooding sessions, showed significant relapse at follow-up."}
{"id": "PMID:469515", "title": "Sleep induced by L-tryptophan. Effect of dosages within the normal dietary intake.", "content": "Previous results have demonstrated sleep-inducing effects of L-tryptophan in doses of 1 to 15 g at bedtime. The present laboratory study extends the dose-response curve downward, comparing doses of 1/4 g, 1/2 g, and 1 g of L-tryptophan with placebo, in 15 mild insomniacs (subjects who reported sleep latencies of over 30 minutes). One gram of L-tryptophan significantly reduced sleep latency: the lower doses produced a trend in the same direction. Stage IV sleep was significantly increased by 1/4 g of L-tryptophan. These results at low doses have interesting implications since the normal dietary intake of L-tryptophan is 1/2 g to 2 g per day.", "contents": "Sleep induced by L-tryptophan. Effect of dosages within the normal dietary intake. Previous results have demonstrated sleep-inducing effects of L-tryptophan in doses of 1 to 15 g at bedtime. The present laboratory study extends the dose-response curve downward, comparing doses of 1/4 g, 1/2 g, and 1 g of L-tryptophan with placebo, in 15 mild insomniacs (subjects who reported sleep latencies of over 30 minutes). One gram of L-tryptophan significantly reduced sleep latency: the lower doses produced a trend in the same direction. Stage IV sleep was significantly increased by 1/4 g of L-tryptophan. These results at low doses have interesting implications since the normal dietary intake of L-tryptophan is 1/2 g to 2 g per day."}
{"id": "PMID:469516", "title": "Recurrent pathological jealousy.", "content": "A 36-year-old man suffered from pathological jealousy during three marriages. The episodes involving delusions of infidelity were usually preceded by premonitory symptoms. At first, the patient responded by discontinuing his medication (a phenothiazine) so that he might remain vigilant. Later he came to recognize the premonitory symptoms as signals indicating a need for help, and he would request hospitalization. In the periods between his marriages he was never troubled by feelings of jealousy. The chief determinant of delusions of infidelity in this instance, as in three reported by Docherty and Ellis, seemed to be exposure to the mother's extramarital sexual relations. This case, in which three different wives were subjected to the same pattern of behavior, would seem to be an exception to the rule that if marital problems occur both partners are responsible for creating them.", "contents": "Recurrent pathological jealousy. A 36-year-old man suffered from pathological jealousy during three marriages. The episodes involving delusions of infidelity were usually preceded by premonitory symptoms. At first, the patient responded by discontinuing his medication (a phenothiazine) so that he might remain vigilant. Later he came to recognize the premonitory symptoms as signals indicating a need for help, and he would request hospitalization. In the periods between his marriages he was never troubled by feelings of jealousy. The chief determinant of delusions of infidelity in this instance, as in three reported by Docherty and Ellis, seemed to be exposure to the mother's extramarital sexual relations. This case, in which three different wives were subjected to the same pattern of behavior, would seem to be an exception to the rule that if marital problems occur both partners are responsible for creating them."}
{"id": "PMID:469517", "title": "Hysterical sleep episodes as a manifestation of masked depression.", "content": "A 55-year-old man suffered from compulsive sleep episodes as a manifestation of masked depression. Although the overt symptoms were conversive in nature, the treatment which finally focused on the depression was followed by disappearance of the patient's recurring sleeping spells for the first time in 4 years. Three methods were used: a) supportive psychotherapy, b) behavior therapy, and c) antidepressive medication. We conclude that the behavior treatment indirectly revealed the masked depression previously concealed by rigid defense mechanisms.", "contents": "Hysterical sleep episodes as a manifestation of masked depression. A 55-year-old man suffered from compulsive sleep episodes as a manifestation of masked depression. Although the overt symptoms were conversive in nature, the treatment which finally focused on the depression was followed by disappearance of the patient's recurring sleeping spells for the first time in 4 years. Three methods were used: a) supportive psychotherapy, b) behavior therapy, and c) antidepressive medication. We conclude that the behavior treatment indirectly revealed the masked depression previously concealed by rigid defense mechanisms."}
{"id": "PMID:469518", "title": "An anticholinergic toxicity reaction to chlorpromazine activated by psychological stress.", "content": "A 42-year-old woman with a long-standing diagnosis of schizophrenia experienced two toxic reactions to 1200 mg of chlorpromazine while awaiting surgery for a locally metastatic carcinoma of the cervix. These reactions, which involved fever, increased pulse and respirations, and acute onset of obtundation, and which cleared after several hours, were produced by a medication and dose which had been well tolerated prior to the preoperative period. These reactions, typical of an acute anticholinergic syndrome, were precipitated by an interaction of the drug and a stress-induced change in her psychological state, due to anxiety about her cancer and impending surgery. This type of reaction has not been reported previously in a clinical setting.", "contents": "An anticholinergic toxicity reaction to chlorpromazine activated by psychological stress. A 42-year-old woman with a long-standing diagnosis of schizophrenia experienced two toxic reactions to 1200 mg of chlorpromazine while awaiting surgery for a locally metastatic carcinoma of the cervix. These reactions, which involved fever, increased pulse and respirations, and acute onset of obtundation, and which cleared after several hours, were produced by a medication and dose which had been well tolerated prior to the preoperative period. These reactions, typical of an acute anticholinergic syndrome, were precipitated by an interaction of the drug and a stress-induced change in her psychological state, due to anxiety about her cancer and impending surgery. This type of reaction has not been reported previously in a clinical setting."}
{"id": "PMID:469519", "title": "Conformational influences on brain tryptophan hydroxylase by submicromolar calcium: opposite effects of equimolar lithium.", "content": "Tryptophan hydroxylase from rat midbrain, EGTA-pretreated and dialyzed, manifested allosteric properties with respect to its substrate tryptophan, cofactor tetrahydrobiopterin, and the calcium ion. Kinetic studies suggest two preferred enzyme conformations in the presence of low concentrations of the cosubstrates: a higher affinity form manifesting hyperbolic substrate kinetics, induced by submicromolar (0.4--0.8 microM) calcium in vitro and cocaine in vivo, and a lower affinity form exaggerating cooperativity with respect to substrate, induced by submicromolar (0.4 to 0.8 microM) lithium in vitro and lithium in vivo. Lithium's effect on serotonin biosynthesis may be due to its antagonism of the positive effector influence of calcium on tryptophan hydroxylase, either as a negative effector or by blocking the calcium site.", "contents": "Conformational influences on brain tryptophan hydroxylase by submicromolar calcium: opposite effects of equimolar lithium. Tryptophan hydroxylase from rat midbrain, EGTA-pretreated and dialyzed, manifested allosteric properties with respect to its substrate tryptophan, cofactor tetrahydrobiopterin, and the calcium ion. Kinetic studies suggest two preferred enzyme conformations in the presence of low concentrations of the cosubstrates: a higher affinity form manifesting hyperbolic substrate kinetics, induced by submicromolar (0.4--0.8 microM) calcium in vitro and cocaine in vivo, and a lower affinity form exaggerating cooperativity with respect to substrate, induced by submicromolar (0.4 to 0.8 microM) lithium in vitro and lithium in vivo. Lithium's effect on serotonin biosynthesis may be due to its antagonism of the positive effector influence of calcium on tryptophan hydroxylase, either as a negative effector or by blocking the calcium site."}
{"id": "PMID:469520", "title": "Secretory activity and histoenzymological characteristics in the subcommissural organ of hormone-injected ducks, Anas platyrhynchos.", "content": "Gonadotrophin and gonadal hormone injections performed on male ducks induce some changes in the activity of the subcommissural organ (SCO). Particularly LH, FSH and testosterone propionate injections are followed by a depletion of secretory material accompanied by increased activities of investigated dehydrogenases and acid phosphatase in SCO cells.", "contents": "Secretory activity and histoenzymological characteristics in the subcommissural organ of hormone-injected ducks, Anas platyrhynchos. Gonadotrophin and gonadal hormone injections performed on male ducks induce some changes in the activity of the subcommissural organ (SCO). Particularly LH, FSH and testosterone propionate injections are followed by a depletion of secretory material accompanied by increased activities of investigated dehydrogenases and acid phosphatase in SCO cells."}
{"id": "PMID:469521", "title": "Location by paper chromatography of compensatory ovarian hypertrophy (COH) inhibiting activity in isobutanol extracts of bovine pineals.", "content": "Bovine pineal glands were extracted according to the methods reported by Bensinger et al. (1973) and Cheesman and Fariss (1970). Isobutanol soluble COH-inhibiting activity was further separated by chromatography on Sephadex G-15 and paper chromatography. With the Bensinger method, different active pineal fractions were obtained from Sephadex G-15 columns. Certain of those fractions were further separated by paper chromatography in butanol : acetic acid : water (4 : 1 : 1) and the COH-inhibitor was localized. The pineal COH-inhibitor could also be localized by high pressure, reverse phase liquid chromatography. More COH-inhibiting activity was extracted with the Bensinger method than with aqueous and acetic acid extraction methods used earlier by us. The Cheesman extraction method for arginine vasotocin gave less regular results in our hands than the Bensinger extraction method.", "contents": "Location by paper chromatography of compensatory ovarian hypertrophy (COH) inhibiting activity in isobutanol extracts of bovine pineals. Bovine pineal glands were extracted according to the methods reported by Bensinger et al. (1973) and Cheesman and Fariss (1970). Isobutanol soluble COH-inhibiting activity was further separated by chromatography on Sephadex G-15 and paper chromatography. With the Bensinger method, different active pineal fractions were obtained from Sephadex G-15 columns. Certain of those fractions were further separated by paper chromatography in butanol : acetic acid : water (4 : 1 : 1) and the COH-inhibitor was localized. The pineal COH-inhibitor could also be localized by high pressure, reverse phase liquid chromatography. More COH-inhibiting activity was extracted with the Bensinger method than with aqueous and acetic acid extraction methods used earlier by us. The Cheesman extraction method for arginine vasotocin gave less regular results in our hands than the Bensinger extraction method."}
{"id": "PMID:469522", "title": "Dietary management of nystagmus.", "content": "Two case reports illustrate the therapeutic response of congenital nystagmus to a diet eliminating synthetic food colors, synthetic food flavors, the antioxidant preservatives butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA), and a small group of foods thought to contain a natural salicylate radical. A brief discussion of the hyperkinetic syndrome is offered with the proposal that a variety of neurologic and neuromuscular disturbances (grand mal, petit mal, psychomotor seizures; La Tourette syndrome; autism; retardation; the behevioral component of Down's syndrome; and oculomotor disturbances) may be induced by identical chemicals, depending upon the individual's genetic profile and the interaction with other environmental factors. It is perhaps the failure to integrate all the signs presented by the various clinical patterns with hyperkinesis or Minimal Brain Dysfunction (MBD) under a single heading that eye muscle involvement manifested as either nystagmus or strabismus has not been emphasized as part of the hyperkinetic syndrome.", "contents": "Dietary management of nystagmus. Two case reports illustrate the therapeutic response of congenital nystagmus to a diet eliminating synthetic food colors, synthetic food flavors, the antioxidant preservatives butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA), and a small group of foods thought to contain a natural salicylate radical. A brief discussion of the hyperkinetic syndrome is offered with the proposal that a variety of neurologic and neuromuscular disturbances (grand mal, petit mal, psychomotor seizures; La Tourette syndrome; autism; retardation; the behevioral component of Down's syndrome; and oculomotor disturbances) may be induced by identical chemicals, depending upon the individual's genetic profile and the interaction with other environmental factors. It is perhaps the failure to integrate all the signs presented by the various clinical patterns with hyperkinesis or Minimal Brain Dysfunction (MBD) under a single heading that eye muscle involvement manifested as either nystagmus or strabismus has not been emphasized as part of the hyperkinetic syndrome."}
{"id": "PMID:469523", "title": "Effects of intranigral administration of dopamine agonists and antagonists and baclofen on concentrations of dopac and dopamine in the striatum and substantia nigra of the rat.", "content": "Systemic administration of haloperidol to rats failed to alter the concentration of dopamine but increased the concentration of 3,4-dihydroxy-phenylacetic acid (DOPAC), the major metabolite of dopamine, in both the striatum and substantia nigra. These haloperidol-induced changes in DOPAC were prevented by an intranigral microinjection of baclofen, a drug which reduces nigrostriatal dopaminergic neuronal activity. It has been proposed that nigrostriatal neurons are regulated, in part, by the activation of autoreceptors in substantia nigra. However, intranigral microinjections of high concentrations of several dopaminergic antagonists failed to mimic the biochemical effects resulting from systemic administration of these drugs; instead, they slightly increased the concentrations of both dopamine and DOPAC in the striatum and decreased dopamine in substantia nigra. Intranigral microinjections of the dopamine agonist, apomorphine, failed to alter the concentration of DOPAC in the striatum, but reduced both dopamine and DOPAC in the nigra. If DOPAC concentrations reflect nigrostriatal dopaminergic neuronal activity, the present results suggest that dopaminergic agonists and antagonists acting in substantia nigra do not exert a major influence on the activity of these neurons.", "contents": "Effects of intranigral administration of dopamine agonists and antagonists and baclofen on concentrations of dopac and dopamine in the striatum and substantia nigra of the rat. Systemic administration of haloperidol to rats failed to alter the concentration of dopamine but increased the concentration of 3,4-dihydroxy-phenylacetic acid (DOPAC), the major metabolite of dopamine, in both the striatum and substantia nigra. These haloperidol-induced changes in DOPAC were prevented by an intranigral microinjection of baclofen, a drug which reduces nigrostriatal dopaminergic neuronal activity. It has been proposed that nigrostriatal neurons are regulated, in part, by the activation of autoreceptors in substantia nigra. However, intranigral microinjections of high concentrations of several dopaminergic antagonists failed to mimic the biochemical effects resulting from systemic administration of these drugs; instead, they slightly increased the concentrations of both dopamine and DOPAC in the striatum and decreased dopamine in substantia nigra. Intranigral microinjections of the dopamine agonist, apomorphine, failed to alter the concentration of DOPAC in the striatum, but reduced both dopamine and DOPAC in the nigra. If DOPAC concentrations reflect nigrostriatal dopaminergic neuronal activity, the present results suggest that dopaminergic agonists and antagonists acting in substantia nigra do not exert a major influence on the activity of these neurons."}
{"id": "PMID:469524", "title": "Effect of quipazine on brain stem monoamine neurons histofluorescence studies.", "content": "Using two fluorescence histochemical methods, formaldehyde-induced fluorescence and sucrose-potassiumphosphate-glyoxylic acid fluorescence (SPG), we studied the effect of 5-hydroxytryptamine receptor stimulation by quipazine (2-[-piperazinyl]quinoline maleate) on monoamine fluorescence in the brain stem of rats. It was found that quipazine in a dose of 5 mg/kg i.p., after 60 min, decreased noradrenaline fluorescence intensity in noradrenergic neurons of the subcoeruleus area and diminished th density of catecholamine terminals visualized in the central part of the dorsal raph\u00e9 nucleus. In the principal locus coeruleus, the intensity of fluorescence in nerve cells was not changed using either method, but with the SPG procedure, diffuse fluorescence outside cell bodies was observed after quipazine. In dorsal raph\u00e9 neurons, a slight increase in 5-hydroxytryptamine fluorescence intensity was observed. The results obtained indicate that quipazine, apart from its effect on 5-hydroxytryptamine neurons, may also affect certain noradrenergic neurons.", "contents": "Effect of quipazine on brain stem monoamine neurons histofluorescence studies. Using two fluorescence histochemical methods, formaldehyde-induced fluorescence and sucrose-potassiumphosphate-glyoxylic acid fluorescence (SPG), we studied the effect of 5-hydroxytryptamine receptor stimulation by quipazine (2-[-piperazinyl]quinoline maleate) on monoamine fluorescence in the brain stem of rats. It was found that quipazine in a dose of 5 mg/kg i.p., after 60 min, decreased noradrenaline fluorescence intensity in noradrenergic neurons of the subcoeruleus area and diminished th density of catecholamine terminals visualized in the central part of the dorsal raph\u00e9 nucleus. In the principal locus coeruleus, the intensity of fluorescence in nerve cells was not changed using either method, but with the SPG procedure, diffuse fluorescence outside cell bodies was observed after quipazine. In dorsal raph\u00e9 neurons, a slight increase in 5-hydroxytryptamine fluorescence intensity was observed. The results obtained indicate that quipazine, apart from its effect on 5-hydroxytryptamine neurons, may also affect certain noradrenergic neurons."}
{"id": "PMID:469525", "title": "The synaptic ribbons of the guinea pig pineal gland in sterile, pregnant and fertile but non-pregnant females and in reproductively active males.", "content": "Pinealocyte ultrastructure has been studied in four sterile, four pregnant and three fertile but non-pregnant females, and also in three reproductively active male pigmented Duncan Hartley guinea pigs. Synaptic ribbons are dense, rod-like structures with a linear arrangement of clear vesicles periodically spaced on both sides of the rodlet. Although these structures were observed in the pinealocytes of all of the animals studied, they were scarce and difficult to locate in tissue from the fertile, non-pregnant females and from the reproductively active males. They were numerous in the pineal glands of the pregnant and sterile females. Typically they lie perpendicular to the cell membrane of the pinealocyte polar process and in close proximity to a polar process of a neighboring cell. The increased incidence of synaptic ribbons in pinealocytes which appear to be in a heightened state of activity strongly suggests a function for this structure. Synaptic ribbons are also present in sensory systems such as rods and cones of the retina, hair cells of the organ of Corti and hair cells of the vestibular apparatus. This fact, plus the photoreceptor function of pinealocytes in lower vertebrates, lends credence to the possibility that this structure may serve a sensory or receptor function in the guinea pig gland.", "contents": "The synaptic ribbons of the guinea pig pineal gland in sterile, pregnant and fertile but non-pregnant females and in reproductively active males. Pinealocyte ultrastructure has been studied in four sterile, four pregnant and three fertile but non-pregnant females, and also in three reproductively active male pigmented Duncan Hartley guinea pigs. Synaptic ribbons are dense, rod-like structures with a linear arrangement of clear vesicles periodically spaced on both sides of the rodlet. Although these structures were observed in the pinealocytes of all of the animals studied, they were scarce and difficult to locate in tissue from the fertile, non-pregnant females and from the reproductively active males. They were numerous in the pineal glands of the pregnant and sterile females. Typically they lie perpendicular to the cell membrane of the pinealocyte polar process and in close proximity to a polar process of a neighboring cell. The increased incidence of synaptic ribbons in pinealocytes which appear to be in a heightened state of activity strongly suggests a function for this structure. Synaptic ribbons are also present in sensory systems such as rods and cones of the retina, hair cells of the organ of Corti and hair cells of the vestibular apparatus. This fact, plus the photoreceptor function of pinealocytes in lower vertebrates, lends credence to the possibility that this structure may serve a sensory or receptor function in the guinea pig gland."}
{"id": "PMID:469526", "title": "Effects of L-proline and some of its analogs on retinal spreading of depression.", "content": "L-Proline inhibits glutamate-based spreading depressions (SDs) at low concentrations (2--2.5 mM) and promotes K+-based SDs at higher concentrations (5 mM). The inhibition of glutamate-based SDs was postulated to be due to competition of L-glutamate and L-proline for glutamate receptors on somatic and dendritic plasma membranes. The binding of proline to glutamate receptors was furthermore postulated to result in a release of K+ from the intracellular compartment, enhancing the extracellular K+ concentration sufficiently to promote K+-based SDs. A proline analog, L-baikiain, containing a double bond and one more C atom in the ring structure than proline had similar effects as the latter amino acid, but an analog, L-azetidine-2-carboxylic acid, with one less C atom in the ring had little effect on SD in the retina.", "contents": "Effects of L-proline and some of its analogs on retinal spreading of depression. L-Proline inhibits glutamate-based spreading depressions (SDs) at low concentrations (2--2.5 mM) and promotes K+-based SDs at higher concentrations (5 mM). The inhibition of glutamate-based SDs was postulated to be due to competition of L-glutamate and L-proline for glutamate receptors on somatic and dendritic plasma membranes. The binding of proline to glutamate receptors was furthermore postulated to result in a release of K+ from the intracellular compartment, enhancing the extracellular K+ concentration sufficiently to promote K+-based SDs. A proline analog, L-baikiain, containing a double bond and one more C atom in the ring structure than proline had similar effects as the latter amino acid, but an analog, L-azetidine-2-carboxylic acid, with one less C atom in the ring had little effect on SD in the retina."}
{"id": "PMID:469527", "title": "Primitive nervous systems: electrical activity in ventral nerve cords of the flatworm, Notoplana acticola.", "content": "Electrical activity evoked in the major cords of the ventral submuscular nerve plexus were measured. Recording and stimulation utilized suction electrodes attached directly to exposed nerve cords. Four categories of potentials have been recorded: (a) short latency spikes which have relatively high thresholds and appear to be single units; (b) short duration fast compound spikes that are made up of a small number of large units; (c) long duration compound potentials that are made up from a large number of smaller units, and (d) small amplitude potentials with long latencies and a characteristic shape. These can be conducted diffusely through the nerve plexus. The first two categories of spikes are called \"fast\" potentials because of their characteristic rise and fall times and the last categories are known as \"diffuse\" potentials. The spikelike fast potentials were only recorded from the main trunks (nerves VI), while diffuse potentials could also be recorded from side branches of these nerves. The diffuse potential appears to be conducted throughout the plexus but preferential conducting pathways occur around lesions. Both diffuse and fast potentials show facilitation of response to repeated stimulation. Facilitation can be demonstrated in the presence of high Mg2+ concentrations. Conductance of the diffuse potential also occurs in the presence of high ambient Mg2+. In Ca2+-free medium containing 1-mM EGTA one can also observe facilitatory events. The possibility of Mg2+-insensitive synapses is discussed.", "contents": "Primitive nervous systems: electrical activity in ventral nerve cords of the flatworm, Notoplana acticola. Electrical activity evoked in the major cords of the ventral submuscular nerve plexus were measured. Recording and stimulation utilized suction electrodes attached directly to exposed nerve cords. Four categories of potentials have been recorded: (a) short latency spikes which have relatively high thresholds and appear to be single units; (b) short duration fast compound spikes that are made up of a small number of large units; (c) long duration compound potentials that are made up from a large number of smaller units, and (d) small amplitude potentials with long latencies and a characteristic shape. These can be conducted diffusely through the nerve plexus. The first two categories of spikes are called \"fast\" potentials because of their characteristic rise and fall times and the last categories are known as \"diffuse\" potentials. The spikelike fast potentials were only recorded from the main trunks (nerves VI), while diffuse potentials could also be recorded from side branches of these nerves. The diffuse potential appears to be conducted throughout the plexus but preferential conducting pathways occur around lesions. Both diffuse and fast potentials show facilitation of response to repeated stimulation. Facilitation can be demonstrated in the presence of high Mg2+ concentrations. Conductance of the diffuse potential also occurs in the presence of high ambient Mg2+. In Ca2+-free medium containing 1-mM EGTA one can also observe facilitatory events. The possibility of Mg2+-insensitive synapses is discussed."}
{"id": "PMID:469528", "title": "Primitive nervous systems: electrophysiology of inhibitory events in flatworm nerve cords.", "content": "Evoked potentials from the major longitudinal nerve cords of Notoplana acticola are potentiated if the preparation is decerebrated or if certain nerves in the ventral submuscular plexus are severed. Concomitant with an increase in amplitude of the response (in some preparations over fourfold) is a decrease in latency of the response and a decrease in the threshold stimulus intensity needed to evoke activity. Evoked activity can also be depressed with moderate increases in stimulus intensity and is induced some distance from the recording site. The depressant effects can be lifted if the brain is bisected, if the contralateral nerve VI is severed close to the brain, and if the commissures between the two nerves VI are cut. The depressant effects of nerves V and VI are additive. Depression and the subsequent lifting of the inhibition occur in Ringer baths containing either normal sea water or equal mixtures of sea water and isotonic MgCl2. The possibility of inhibitory synapses immune to high concentrations of magnesium ions is discussed.", "contents": "Primitive nervous systems: electrophysiology of inhibitory events in flatworm nerve cords. Evoked potentials from the major longitudinal nerve cords of Notoplana acticola are potentiated if the preparation is decerebrated or if certain nerves in the ventral submuscular plexus are severed. Concomitant with an increase in amplitude of the response (in some preparations over fourfold) is a decrease in latency of the response and a decrease in the threshold stimulus intensity needed to evoke activity. Evoked activity can also be depressed with moderate increases in stimulus intensity and is induced some distance from the recording site. The depressant effects can be lifted if the brain is bisected, if the contralateral nerve VI is severed close to the brain, and if the commissures between the two nerves VI are cut. The depressant effects of nerves V and VI are additive. Depression and the subsequent lifting of the inhibition occur in Ringer baths containing either normal sea water or equal mixtures of sea water and isotonic MgCl2. The possibility of inhibitory synapses immune to high concentrations of magnesium ions is discussed."}
{"id": "PMID:469529", "title": "Functional characterization of the neurodepressing hormone in the crayfish.", "content": "The effect of the neurodepressing hormone (NDH) was studied on different identified motoneurons in the abdominal ganglia of the crayfish Procambarus bouvieri (Ortmann). Although differences in sensitivity were apparent, all the neurons tested responded to NDH with a reduction in spontaneous firing rate, which lasted as long as NDH was present, and, depending on the concentration and time of action of the hormone, for even longer periods. NDH activity was determined in the various parts of the central nervous system of the crayfish, being highest in the eyestalk, gradually diminishing away from the eyestalk, with a cephalo-caudal gradient, being lowest in the abdominal ganglia. High levels of NDH activity were detected in the blood. After eyestalk ablation, NDH concentration steadily diminishes in the blood and central nervous system, until virtually disappearing after 4 days; from day 5 onwards, the activity is recovered up to its original levels. NDH synthesis takes place with a time constant of approximately 3 hr in cultured isolated segments of central nervous system, being highest in the eyestalk.", "contents": "Functional characterization of the neurodepressing hormone in the crayfish. The effect of the neurodepressing hormone (NDH) was studied on different identified motoneurons in the abdominal ganglia of the crayfish Procambarus bouvieri (Ortmann). Although differences in sensitivity were apparent, all the neurons tested responded to NDH with a reduction in spontaneous firing rate, which lasted as long as NDH was present, and, depending on the concentration and time of action of the hormone, for even longer periods. NDH activity was determined in the various parts of the central nervous system of the crayfish, being highest in the eyestalk, gradually diminishing away from the eyestalk, with a cephalo-caudal gradient, being lowest in the abdominal ganglia. High levels of NDH activity were detected in the blood. After eyestalk ablation, NDH concentration steadily diminishes in the blood and central nervous system, until virtually disappearing after 4 days; from day 5 onwards, the activity is recovered up to its original levels. NDH synthesis takes place with a time constant of approximately 3 hr in cultured isolated segments of central nervous system, being highest in the eyestalk."}
{"id": "PMID:469530", "title": "Bis(L-cysteinato)gold(I): chemical characterization and identification in renal cortical cytoplasm.", "content": "L-Cysteinatogold(I) was prepared by the reaction of L-cysteine with KAuBr4 in acidic media and its solubility determined from pH 4 to 10. The solubility at pH 7.4 and 37 degrees C is 1 microM. In the presence of excess cysteine, the solubility increases because of formation of bis(L-cysteinato)gold(I). The equilibrium-constant for formation of the bis complex is 2.1 +/- 0.4 X 10(-3), which at pH 7.4 CORRESPONDs to an apparant formation constant of 4.4 X10(4). The formation of the bis adduct was confirmed by chromatographic separation of the products of the reaction between [35S]-L-cysteine and Na2AuTM. This complex elutes with Kav = 1.15 which allows it to be distinguished from other gold thiolates that might form in vivo. The bis(cysteinato)gold(I) complex is shown to be present in kidney cytosol isolated from rats given Na2AuTM in vivo. When additional cysteine is added to the cytosol in vitro, the peak at 1.15 is increased, but if glutathione is added, the low molecular weight gold elutes at Kav = 1.00, which is taken as evidence for the existence of bis(cysteinato)gold(I) in the cytosol preparation. The amount of gold present as bis(cysteinato)gold(I) after 4 different dose schedules has been measured and found to increase with the total cytosol gold concentration. L-Cysteinatogold(I) does not dissolve in the presence of bovine serum albumin to form an adduct.", "contents": "Bis(L-cysteinato)gold(I): chemical characterization and identification in renal cortical cytoplasm. L-Cysteinatogold(I) was prepared by the reaction of L-cysteine with KAuBr4 in acidic media and its solubility determined from pH 4 to 10. The solubility at pH 7.4 and 37 degrees C is 1 microM. In the presence of excess cysteine, the solubility increases because of formation of bis(L-cysteinato)gold(I). The equilibrium-constant for formation of the bis complex is 2.1 +/- 0.4 X 10(-3), which at pH 7.4 CORRESPONDs to an apparant formation constant of 4.4 X10(4). The formation of the bis adduct was confirmed by chromatographic separation of the products of the reaction between [35S]-L-cysteine and Na2AuTM. This complex elutes with Kav = 1.15 which allows it to be distinguished from other gold thiolates that might form in vivo. The bis(cysteinato)gold(I) complex is shown to be present in kidney cytosol isolated from rats given Na2AuTM in vivo. When additional cysteine is added to the cytosol in vitro, the peak at 1.15 is increased, but if glutathione is added, the low molecular weight gold elutes at Kav = 1.00, which is taken as evidence for the existence of bis(cysteinato)gold(I) in the cytosol preparation. The amount of gold present as bis(cysteinato)gold(I) after 4 different dose schedules has been measured and found to increase with the total cytosol gold concentration. L-Cysteinatogold(I) does not dissolve in the presence of bovine serum albumin to form an adduct."}
{"id": "PMID:469555", "title": "Difficulties in diagnosis of supratentorial gliomas by CAT scan.", "content": "The false positive and false negative computed tomography diagnoses of glioma made using an EMI 1010 machine on a consecutive series of patients seen over a period of two years are recorded. About 1.5% of gliomas were not detected on initial CAT scan, 6.5% were misdiagnosed as benign lesions, and in 6.5% of the cases identified as glioma a non-malignant condition was subsequently diagnosed.", "contents": "Difficulties in diagnosis of supratentorial gliomas by CAT scan. The false positive and false negative computed tomography diagnoses of glioma made using an EMI 1010 machine on a consecutive series of patients seen over a period of two years are recorded. About 1.5% of gliomas were not detected on initial CAT scan, 6.5% were misdiagnosed as benign lesions, and in 6.5% of the cases identified as glioma a non-malignant condition was subsequently diagnosed."}
{"id": "PMID:469551", "title": "Ultraviolet-mediated cytotoxic activity of phenylheptatriyne from Bidens pilosa L.", "content": "The tropical weed Bidens pilosa L. (Asteraceae) contains a number of polyacetylenes which are phototoxic to bacteria, fungi, and human fibroblast cells in the presence of sunlight, artificial sources of long-wave ultraviolet light, or cool-white fluorescent light. The principle photoactive compound in the leaf, phenylheptatriyne, is present in the cuticle as well as in the underlying cells. Experiments with calf thymus DNA indicate that, unlike photoactive furanocoumarins, phenylheptatriyne does not form interstrand cross linkages with DNA in ultraviolet light.", "contents": "Ultraviolet-mediated cytotoxic activity of phenylheptatriyne from Bidens pilosa L. The tropical weed Bidens pilosa L. (Asteraceae) contains a number of polyacetylenes which are phototoxic to bacteria, fungi, and human fibroblast cells in the presence of sunlight, artificial sources of long-wave ultraviolet light, or cool-white fluorescent light. The principle photoactive compound in the leaf, phenylheptatriyne, is present in the cuticle as well as in the underlying cells. Experiments with calf thymus DNA indicate that, unlike photoactive furanocoumarins, phenylheptatriyne does not form interstrand cross linkages with DNA in ultraviolet light."}
{"id": "PMID:469556", "title": "Papilloedema, CSF pressure, and CSF flow in cerebral tumours.", "content": "The presence or absence of papilloedema and the cisternal CSF pressure were compared with the RIHSA cisternograms in 24 patients with supratenotorial tumours. It was found that severe subarachnoid obstruction of CSF flow, leading to impaired CSF absorption at the superior sagittal sinus, was the main cause of raised CSF pressure and papilloedema associated with supratentorial tumours.", "contents": "Papilloedema, CSF pressure, and CSF flow in cerebral tumours. The presence or absence of papilloedema and the cisternal CSF pressure were compared with the RIHSA cisternograms in 24 patients with supratenotorial tumours. It was found that severe subarachnoid obstruction of CSF flow, leading to impaired CSF absorption at the superior sagittal sinus, was the main cause of raised CSF pressure and papilloedema associated with supratentorial tumours."}
{"id": "PMID:469552", "title": "Characterization of an irritant 4-deoxyphorbol diester from Euphorbia tirucalli.", "content": "Latex of Euphorbia tirucalli, collected in Colombia, yielded 12-O-2Z-4E-octadienoyl-4-deoxyphorbol-13-acetate (1), which exhibited an irritant potency equivalent to that of the standard irritant, phorbol-12-tetradecanoate-13-acetate, in a mouse ear test system. The unsaturated 4-deoxyphorbol diesters recently reported in E. tirucalli grown in South Africa were not observed in the present study.", "contents": "Characterization of an irritant 4-deoxyphorbol diester from Euphorbia tirucalli. Latex of Euphorbia tirucalli, collected in Colombia, yielded 12-O-2Z-4E-octadienoyl-4-deoxyphorbol-13-acetate (1), which exhibited an irritant potency equivalent to that of the standard irritant, phorbol-12-tetradecanoate-13-acetate, in a mouse ear test system. The unsaturated 4-deoxyphorbol diesters recently reported in E. tirucalli grown in South Africa were not observed in the present study."}
{"id": "PMID:469557", "title": "Dementia paralytica: deterioration from communicating hydrocephalus.", "content": "Five patients suffering from dementia paralytica who failed to improve or deteriorated after one or several high dosage courses of penicillin, had pneumoencephalographic patterns suggesting communicating hydrocephalus. Measurements of the ventricular index, ratio of cella media to width of the temporal horn, and the callosal angle differed from that in seven cases of dementia paralytica with associated cerebral atrophy. An isotope cisternogram in three cases with communicating hydrocephalus further confirmed a blockage of the cerebrospinal fluid (CSF) at the parasagittal subarachnoid space. Three patients exhibited the full syndrome of gait apraxia, incontinence, and pyramidal tract signs associated with a severe degree of dementia. Shunting of the CSF in three cases was followed by immediate improvement in two, one in a longlasting way. No active parenchymal inflammation was observed in any of three brain biopsy samples taken during surgery, except for leptomeningeal fibrosis in one. Chronic leptomeningitis in dementia paralytica may impair subarachnoid CSF absorption with subsequent communicating hydrocephalus. Progression or inadequate responses after therapeutic dose of penicillin in dementia paralytica should prompt investigation for this complication as an alternative, effective treatment could be offered.", "contents": "Dementia paralytica: deterioration from communicating hydrocephalus. Five patients suffering from dementia paralytica who failed to improve or deteriorated after one or several high dosage courses of penicillin, had pneumoencephalographic patterns suggesting communicating hydrocephalus. Measurements of the ventricular index, ratio of cella media to width of the temporal horn, and the callosal angle differed from that in seven cases of dementia paralytica with associated cerebral atrophy. An isotope cisternogram in three cases with communicating hydrocephalus further confirmed a blockage of the cerebrospinal fluid (CSF) at the parasagittal subarachnoid space. Three patients exhibited the full syndrome of gait apraxia, incontinence, and pyramidal tract signs associated with a severe degree of dementia. Shunting of the CSF in three cases was followed by immediate improvement in two, one in a longlasting way. No active parenchymal inflammation was observed in any of three brain biopsy samples taken during surgery, except for leptomeningeal fibrosis in one. Chronic leptomeningitis in dementia paralytica may impair subarachnoid CSF absorption with subsequent communicating hydrocephalus. Progression or inadequate responses after therapeutic dose of penicillin in dementia paralytica should prompt investigation for this complication as an alternative, effective treatment could be offered."}
{"id": "PMID:469553", "title": "Biological and phytochemical investigation of plants XV. Pteryxia terebinthina var. terebinthina (Umbelliferae).", "content": "Aqueous extracts of Pteryxia terebinthina var. terebinthina were shown to possess antiarrhythmic activity. A novel furanocoumarin, pterybinthinone, the known coumarins pteryxin and umbelliferone, and sucrose were isolated from this plant. Attempts to re-isolate a structurally undefined, defibrillatory substance by means of a published protocol were unsuccessful.", "contents": "Biological and phytochemical investigation of plants XV. Pteryxia terebinthina var. terebinthina (Umbelliferae). Aqueous extracts of Pteryxia terebinthina var. terebinthina were shown to possess antiarrhythmic activity. A novel furanocoumarin, pterybinthinone, the known coumarins pteryxin and umbelliferone, and sucrose were isolated from this plant. Attempts to re-isolate a structurally undefined, defibrillatory substance by means of a published protocol were unsuccessful."}
{"id": "PMID:469558", "title": "Early and preventable changes of peripheral nerve structure and function in insulin-deficient diabetic rats.", "content": "Structure and function of peripheral nerve in early insulin deficient streptozotocin diabetic rat were examined. Quantitative studies of myelinated fibres from electron micrographs showed that the fibre calibre and slope coefficients of regression lines between axon and myelin areas were reduced and quantitation of unmyelinated fibres revealed a 40% reduction in area fraction of smooth endoplasmic reticulum. The difference in conduction velocity between diabetic rats and control rats increased with time just as does the difference in fibre calibre. Insulin treatment was able to prevent the decrease in conduction velocity as well as the reduction in fibre calibre. It is concluded that the early structural axon abnormalities results from the diabetic state and cannot be explained by poor general condition of the animals.", "contents": "Early and preventable changes of peripheral nerve structure and function in insulin-deficient diabetic rats. Structure and function of peripheral nerve in early insulin deficient streptozotocin diabetic rat were examined. Quantitative studies of myelinated fibres from electron micrographs showed that the fibre calibre and slope coefficients of regression lines between axon and myelin areas were reduced and quantitation of unmyelinated fibres revealed a 40% reduction in area fraction of smooth endoplasmic reticulum. The difference in conduction velocity between diabetic rats and control rats increased with time just as does the difference in fibre calibre. Insulin treatment was able to prevent the decrease in conduction velocity as well as the reduction in fibre calibre. It is concluded that the early structural axon abnormalities results from the diabetic state and cannot be explained by poor general condition of the animals."}
{"id": "PMID:469554", "title": "Antineoplastic activity and cytotoxicity of flavones, isoflavones, and flavanones.", "content": "Several hundred flavonoid derivatives, natural and synthetic, which have been tested in the screening program of the National Cancer Institute, have been examined for indications of structure-activity relationships which might exist among these compounds. No such relationships are apparent. In spite of occasional activity these compounds do not warrant further detailed pursuit as anti-tumor agents.", "contents": "Antineoplastic activity and cytotoxicity of flavones, isoflavones, and flavanones. Several hundred flavonoid derivatives, natural and synthetic, which have been tested in the screening program of the National Cancer Institute, have been examined for indications of structure-activity relationships which might exist among these compounds. No such relationships are apparent. In spite of occasional activity these compounds do not warrant further detailed pursuit as anti-tumor agents."}
{"id": "PMID:469559", "title": "Malignant mesenchymoma of ulnar nerve: combined sarcoma of nerve sheath and rhabdomyosarcoma.", "content": "Malignant mesenchymoma within the right ulnar nerve of an 8 year old boy is described. The patient did not have stigmata of von Recklinghausen's neurofibromatosis. The growing and painful tumour was excised five and a half detection, and recurred five months later. Mingling of the nerve sheath sarcoma and rhabdomyosarcoma was noted within the same mass which was separated from the adjacent striated muscles. It is suggested that this mesenchymoma arose from mesenchymal cells or cells of mesenchymal type comprising the peripheral nerve sheath which is derived from ectomesenchyme of the neural crest.", "contents": "Malignant mesenchymoma of ulnar nerve: combined sarcoma of nerve sheath and rhabdomyosarcoma. Malignant mesenchymoma within the right ulnar nerve of an 8 year old boy is described. The patient did not have stigmata of von Recklinghausen's neurofibromatosis. The growing and painful tumour was excised five and a half detection, and recurred five months later. Mingling of the nerve sheath sarcoma and rhabdomyosarcoma was noted within the same mass which was separated from the adjacent striated muscles. It is suggested that this mesenchymoma arose from mesenchymal cells or cells of mesenchymal type comprising the peripheral nerve sheath which is derived from ectomesenchyme of the neural crest."}
{"id": "PMID:469560", "title": "Xanthochromic cysts associated with meningioma.", "content": "Three cases of cystic meningioma encountered in one year are presented. It appears from a review of the literature, and an analysis of these three cases, that large xanthochromic cerebral cysts may be associated with meningiomas in any of three configurations: (1) centrally within the tumour; (2) peripherally within the tumour; (3) in the adjacent brain. Regardless of which configuration applies, the CAT scan appearance of such cystic meningiomas may mimic that of a glial tumour with cystic or necrotic change, and lead to an incorrect presumptive diagnosis. This false impression may be perpetuated by the gross appearance at operation, which can also mimic malignant glioma. Although several radiological features should suggest the possible presence of a cystic meningioma, we know of no definite radiological means of differentiating this lesion from the more common malignant glioma. This finding should underline the need to biopsy all suspected cerebral neoplasms, regardless of how much their appearance on CAT scan may suggest malignant glioma.", "contents": "Xanthochromic cysts associated with meningioma. Three cases of cystic meningioma encountered in one year are presented. It appears from a review of the literature, and an analysis of these three cases, that large xanthochromic cerebral cysts may be associated with meningiomas in any of three configurations: (1) centrally within the tumour; (2) peripherally within the tumour; (3) in the adjacent brain. Regardless of which configuration applies, the CAT scan appearance of such cystic meningiomas may mimic that of a glial tumour with cystic or necrotic change, and lead to an incorrect presumptive diagnosis. This false impression may be perpetuated by the gross appearance at operation, which can also mimic malignant glioma. Although several radiological features should suggest the possible presence of a cystic meningioma, we know of no definite radiological means of differentiating this lesion from the more common malignant glioma. This finding should underline the need to biopsy all suspected cerebral neoplasms, regardless of how much their appearance on CAT scan may suggest malignant glioma."}
{"id": "PMID:469561", "title": "3-Methylhistidine excretion as an index of myofibrillar protein catabolism in neuromuscular disease.", "content": "Myofibrillar protein catabolism has been calculated in a variety of neuromuscular diseases from the amount of 3-methylhistidine excreted in the urine. It was found to be significantly raised in Duchenne type muscular dystrophy, motor neurone disease, polymyositis, and thyrotoxic  myopathy. In Becker type muscular dystrophy the level was slightly raised. It was normal in scapuloperoneal and limb girdle dystrophy, dystrophia myotonica, extrapyramidal disease, and multiple sclerosis. It was significantly decreased in hypothyroid myopathy.", "contents": "3-Methylhistidine excretion as an index of myofibrillar protein catabolism in neuromuscular disease. Myofibrillar protein catabolism has been calculated in a variety of neuromuscular diseases from the amount of 3-methylhistidine excreted in the urine. It was found to be significantly raised in Duchenne type muscular dystrophy, motor neurone disease, polymyositis, and thyrotoxic  myopathy. In Becker type muscular dystrophy the level was slightly raised. It was normal in scapuloperoneal and limb girdle dystrophy, dystrophia myotonica, extrapyramidal disease, and multiple sclerosis. It was significantly decreased in hypothyroid myopathy."}
{"id": "PMID:469562", "title": "Centronuclear myopathy.", "content": "Centronuclear myopathy occurring sporadically in two African female children is reported, with details of clinical history and histological, histochemical, and ultrastructural findings, and a review of 58 previously reported cases. In spite of distinctive histological features, the clinical presentation of this condition is variable, there are different modes of inheritance, and the pathogenesis remains unclear.", "contents": "Centronuclear myopathy. Centronuclear myopathy occurring sporadically in two African female children is reported, with details of clinical history and histological, histochemical, and ultrastructural findings, and a review of 58 previously reported cases. In spite of distinctive histological features, the clinical presentation of this condition is variable, there are different modes of inheritance, and the pathogenesis remains unclear."}
{"id": "PMID:469563", "title": "Myokymia and impaired muscular relaxation with continuous motor unit activity.", "content": "We have studied two cases of the syndrome of myokymia and impaired muscular relaxation with continuous motor unit activity. Both patients complained of muscle twitching, weakness, stiffness, and hyperhydrosis during their illness. Myokymia was present over the entire body in both. On repetitive testing of muscle strength each patient showed initial fatigue followed by increasing strength as he continued his efforts. Both patinets improved on phenytoin therapy at high blood levels. Nerve conduction velocities were decreased. Electromyograms showed continuous electrical activity at rest which persisted during sleep and spinal anaesthesia but was diminished by curare. Intravital staining with methylene blue in one case demonstrated sprouting and beading of motor nerve terminals with multiple innervation of muscle fibres. The neurophysiological and pathological findings in these two cases indicate an abnormality of peripheral nerve in this disorder.", "contents": "Myokymia and impaired muscular relaxation with continuous motor unit activity. We have studied two cases of the syndrome of myokymia and impaired muscular relaxation with continuous motor unit activity. Both patients complained of muscle twitching, weakness, stiffness, and hyperhydrosis during their illness. Myokymia was present over the entire body in both. On repetitive testing of muscle strength each patient showed initial fatigue followed by increasing strength as he continued his efforts. Both patinets improved on phenytoin therapy at high blood levels. Nerve conduction velocities were decreased. Electromyograms showed continuous electrical activity at rest which persisted during sleep and spinal anaesthesia but was diminished by curare. Intravital staining with methylene blue in one case demonstrated sprouting and beading of motor nerve terminals with multiple innervation of muscle fibres. The neurophysiological and pathological findings in these two cases indicate an abnormality of peripheral nerve in this disorder."}
{"id": "PMID:469564", "title": "Assessment of directional Doppler ultrasound techniques in the diagnosis of carotid artery diseases.", "content": "Three diagnostic techniques based on the use of directional doppler ultrasound have been evaluated in comparison with direct percutaneous carotid angiography and arch aortography. The results indicate that the non-invasive techniques provide a safe, atraumatic method of assessing patients with symptoms of extracranial occlusive arterial disease.", "contents": "Assessment of directional Doppler ultrasound techniques in the diagnosis of carotid artery diseases. Three diagnostic techniques based on the use of directional doppler ultrasound have been evaluated in comparison with direct percutaneous carotid angiography and arch aortography. The results indicate that the non-invasive techniques provide a safe, atraumatic method of assessing patients with symptoms of extracranial occlusive arterial disease."}
{"id": "PMID:469565", "title": "Visual perseveration in temporal lobe epilepsy.", "content": "Ictal visual perseveration is reported in two patients with temporal lobe epilepsy. A distinction is drawn between visual perseveration and palinopsia, or recurrence of a visual image.", "contents": "Visual perseveration in temporal lobe epilepsy. Ictal visual perseveration is reported in two patients with temporal lobe epilepsy. A distinction is drawn between visual perseveration and palinopsia, or recurrence of a visual image."}
{"id": "PMID:469566", "title": "Transient global amnesia: an extensive case report.", "content": "A case of transient global amnesia was studied in detail. Of note was a temporally extensive, although quite patchy, retrograde amnesia during the event for information recallable at other times. While a consolidation block can explain the anterograde amnesia in transient global amnesia, the profound retrograde amnesia requires an additional block in the retrieval of old, established memories.", "contents": "Transient global amnesia: an extensive case report. A case of transient global amnesia was studied in detail. Of note was a temporally extensive, although quite patchy, retrograde amnesia during the event for information recallable at other times. While a consolidation block can explain the anterograde amnesia in transient global amnesia, the profound retrograde amnesia requires an additional block in the retrieval of old, established memories."}
{"id": "PMID:469567", "title": "Lead-induced encephalopathy in dogs fed high fat, low calcium diets.", "content": "Growing, mongrel dogs were fed high fat (22%), low calcium (.1%) semipurified and purified diets with and without 77 ppm lead as lead acetate to experimentally induce the seizures and lead encephalopathy historically associated with accidental canine lead toxicity. Seizures were observed in 44% of the lead toxic dogs and microscopic encephalopathy was observed in 89% of the lead toxic dogs. The encephalopathy was characterized by bilaterally symmetrical areas of vacuole formation involving the neuropile especially in a laminar pattern at the tips of the gyri of the cerebral cortex. The spongy state was accompanied by capillary activation and gliosis. These lesions are similar to those reported in accidental lead toxicoses in other species but previous efforts to experimentally induce these lesions in young dogs fed low-calcium, normal-fat (16%) purified diets have been unsuccessful.", "contents": "Lead-induced encephalopathy in dogs fed high fat, low calcium diets. Growing, mongrel dogs were fed high fat (22%), low calcium (.1%) semipurified and purified diets with and without 77 ppm lead as lead acetate to experimentally induce the seizures and lead encephalopathy historically associated with accidental canine lead toxicity. Seizures were observed in 44% of the lead toxic dogs and microscopic encephalopathy was observed in 89% of the lead toxic dogs. The encephalopathy was characterized by bilaterally symmetrical areas of vacuole formation involving the neuropile especially in a laminar pattern at the tips of the gyri of the cerebral cortex. The spongy state was accompanied by capillary activation and gliosis. These lesions are similar to those reported in accidental lead toxicoses in other species but previous efforts to experimentally induce these lesions in young dogs fed low-calcium, normal-fat (16%) purified diets have been unsuccessful."}
{"id": "PMID:469568", "title": "A quantitative study of the pigmented neurons in the nuclei locus coeruleus and subcoeruleus in man as related to aging.", "content": "The nuclei of the locus coeruleus and subcoeruleus were examined in human brains from individuals of 14 to 87 years. Serial counts were performed on every tenth Nissl stained paraffin section of melanin containing cells within the entire length of these nuclei. After 63 years, the neuronal population in these nuclei decreased 40%. This significant decrease in cell number is the first reported for a brain stem structure. In view of the relation of these noradrenaline containing cells to the catecholaminergic system and the widespread projections between these nuclei and other central nervous system structures, this decrease may be a factor in alteration of some central nervous system activities with increasing age.", "contents": "A quantitative study of the pigmented neurons in the nuclei locus coeruleus and subcoeruleus in man as related to aging. The nuclei of the locus coeruleus and subcoeruleus were examined in human brains from individuals of 14 to 87 years. Serial counts were performed on every tenth Nissl stained paraffin section of melanin containing cells within the entire length of these nuclei. After 63 years, the neuronal population in these nuclei decreased 40%. This significant decrease in cell number is the first reported for a brain stem structure. In view of the relation of these noradrenaline containing cells to the catecholaminergic system and the widespread projections between these nuclei and other central nervous system structures, this decrease may be a factor in alteration of some central nervous system activities with increasing age."}
{"id": "PMID:469569", "title": "Peripheral nerve demyelination induced by intraneural injection of experimental allergic encephalomyelitis serum.", "content": "Intraneural injection of sera from rabbits with experimental allergic encephalomyelitis, induced by sensitization with bovine brain white matter in complete Freund's adjuvant, produced focal primary demyelinative lesions in rat sciatic nerves. Demyelinating activity was removed by prior incubation of antisera with central (CNS) and peripheral nervous system (PNS) myelin but not with liver or kidney, and was heat-labile and complement-dependent. Recipient animals developed a sensorimotor disturbance of their toes and ankles on the side injected with antiserum. Twenty minutes after antiserum injection, Schwann cells showed focal cytoplasmic outpouching and their external mesaxons opened. Between 1 and 8 hours after injection vacuolation, splitting and vesiculation of myelin became increasingly prominent at Schmidt-Lanterman clefts and paranodal regions, with concomitant degenerative changes in Schwann cell cytoplasm. Polymorphonuclear cell infiltration and endoneurial edema were apparent at this time. Substantial demyelination occurred before the appearance of phagocytic cells. Between 8 hours and 3 days many nerve fibers were surrounded and attacked by invading macrophages. Axons became demyelinated progressively over several internodes by macrophage phagocytosis. Early signs of remyelination were observed by 5 days. These findings suggest that antibodies directed against antigens common to both CNS and PNS myelin can produce in vivo peripheral nerve demyelination.", "contents": "Peripheral nerve demyelination induced by intraneural injection of experimental allergic encephalomyelitis serum. Intraneural injection of sera from rabbits with experimental allergic encephalomyelitis, induced by sensitization with bovine brain white matter in complete Freund's adjuvant, produced focal primary demyelinative lesions in rat sciatic nerves. Demyelinating activity was removed by prior incubation of antisera with central (CNS) and peripheral nervous system (PNS) myelin but not with liver or kidney, and was heat-labile and complement-dependent. Recipient animals developed a sensorimotor disturbance of their toes and ankles on the side injected with antiserum. Twenty minutes after antiserum injection, Schwann cells showed focal cytoplasmic outpouching and their external mesaxons opened. Between 1 and 8 hours after injection vacuolation, splitting and vesiculation of myelin became increasingly prominent at Schmidt-Lanterman clefts and paranodal regions, with concomitant degenerative changes in Schwann cell cytoplasm. Polymorphonuclear cell infiltration and endoneurial edema were apparent at this time. Substantial demyelination occurred before the appearance of phagocytic cells. Between 8 hours and 3 days many nerve fibers were surrounded and attacked by invading macrophages. Axons became demyelinated progressively over several internodes by macrophage phagocytosis. Early signs of remyelination were observed by 5 days. These findings suggest that antibodies directed against antigens common to both CNS and PNS myelin can produce in vivo peripheral nerve demyelination."}
{"id": "PMID:469570", "title": "Double replica technique applied to choroid plexus from early foetal sheep: completeness and complexity of tight junctions.", "content": "Choroid plexuses from early and late sheep foetuses were examined by an improved freeze-fracture technique and the use of double-replicas to define the structure of the tight junction network of the epithelial cells. 'Complex' strands which consist of two normal parallel strands separated by a single row of pits or particles are defined and demonstrated in complementary faces. Since this strand variety was encountered in the same proportion in the different development stages investigated, it could not be correlated with changes in permeability. It is more likely that the 'complex' strands are associated with the transfer of gap junction particles within the membrane. The question of the significance of discontinuities in P face ridges was also resolved by the double replica technique: the few discontinuities which were observed could be accounted for by particles in the complementary E faces. Furthermore, approximately the same number of such junction displacements was found in early and late stages which makes it unlikely that this phenomenon could contribute to changes in permeability. Thus it has not been possible so far to relate any structural feature of the tight junction network in developing choroid plexus epithelial cells with the reported changes in permeability of the blood-C.S.F. barrier.", "contents": "Double replica technique applied to choroid plexus from early foetal sheep: completeness and complexity of tight junctions. Choroid plexuses from early and late sheep foetuses were examined by an improved freeze-fracture technique and the use of double-replicas to define the structure of the tight junction network of the epithelial cells. 'Complex' strands which consist of two normal parallel strands separated by a single row of pits or particles are defined and demonstrated in complementary faces. Since this strand variety was encountered in the same proportion in the different development stages investigated, it could not be correlated with changes in permeability. It is more likely that the 'complex' strands are associated with the transfer of gap junction particles within the membrane. The question of the significance of discontinuities in P face ridges was also resolved by the double replica technique: the few discontinuities which were observed could be accounted for by particles in the complementary E faces. Furthermore, approximately the same number of such junction displacements was found in early and late stages which makes it unlikely that this phenomenon could contribute to changes in permeability. Thus it has not been possible so far to relate any structural feature of the tight junction network in developing choroid plexus epithelial cells with the reported changes in permeability of the blood-C.S.F. barrier."}
{"id": "PMID:469571", "title": "Ultrastructure and quantitative synaptology of the sacral parasympathetic nucleus.", "content": "This study examines the anatomical substrate for the spinal micturition reflex. Light microscopy of pyridine silver-stained sections revealed that the sacral parasympathetic nucleus (SPN) exists as a broken column or chain of cell clusters located along the intermediolateral portion of the dorsal horn in sacral segments S2-S4. Quantitative analysis of neuropil components in electron micrographs provides data for each type of bouton identified in this nucleus. On the somata of these neurons, boutons containing clear spherical vesicles (S type) comprise 70% of the bouton population. Terminals containing three or more dense core vesicles (GS boutons) account for 26% and boutons containing flattened vesicles (F boutons) comprise 4% of the population. F boutons are more common on large dendrites where they comprise 10% of the total bouton population. The actual population density of each bouton type is most evident when the number of boutons is expressed as boutons per 100 micron of membrane length (btn/100 micron). S type boutons are the most frequently encountered type. The population density of S boutons is the same on soma and dendrites at 6.66 btn/100 micron. F boutons are more numerous on large (greater than 2 micron) dendrites (1.28 btn/100 micron) than on small dendrites (0.63 btn/100 micron) or on somata (0.36 btn/100 micron). GS boutons occur more frequently on small dendrites (3.66 btn/100 micron) than on somata (2.29 btn/100 micron), large dendrites (2.88 btn/100 micron) or medium dendrites (2.27 btn/100 micron). These data suggest that the dense core vesicle-containing boutons are applied primarily to small (less than 1 micron) dendrites and that F boutons are associated mostly with large or proximal dendrites. These results provide a quantitative profile of the synaptic input to the sacral autonomic (parasympathetic) neurons which innervate the urinary bladder and demonstrate specific population differences on various postsynaptic structures in this nucleus.", "contents": "Ultrastructure and quantitative synaptology of the sacral parasympathetic nucleus. This study examines the anatomical substrate for the spinal micturition reflex. Light microscopy of pyridine silver-stained sections revealed that the sacral parasympathetic nucleus (SPN) exists as a broken column or chain of cell clusters located along the intermediolateral portion of the dorsal horn in sacral segments S2-S4. Quantitative analysis of neuropil components in electron micrographs provides data for each type of bouton identified in this nucleus. On the somata of these neurons, boutons containing clear spherical vesicles (S type) comprise 70% of the bouton population. Terminals containing three or more dense core vesicles (GS boutons) account for 26% and boutons containing flattened vesicles (F boutons) comprise 4% of the population. F boutons are more common on large dendrites where they comprise 10% of the total bouton population. The actual population density of each bouton type is most evident when the number of boutons is expressed as boutons per 100 micron of membrane length (btn/100 micron). S type boutons are the most frequently encountered type. The population density of S boutons is the same on soma and dendrites at 6.66 btn/100 micron. F boutons are more numerous on large (greater than 2 micron) dendrites (1.28 btn/100 micron) than on small dendrites (0.63 btn/100 micron) or on somata (0.36 btn/100 micron). GS boutons occur more frequently on small dendrites (3.66 btn/100 micron) than on somata (2.29 btn/100 micron), large dendrites (2.88 btn/100 micron) or medium dendrites (2.27 btn/100 micron). These data suggest that the dense core vesicle-containing boutons are applied primarily to small (less than 1 micron) dendrites and that F boutons are associated mostly with large or proximal dendrites. These results provide a quantitative profile of the synaptic input to the sacral autonomic (parasympathetic) neurons which innervate the urinary bladder and demonstrate specific population differences on various postsynaptic structures in this nucleus."}
{"id": "PMID:469572", "title": "Ultrastructure of photoreceptors in the eye of Hermissenda labelled with intracellular injections of horseradish peroxidase.", "content": "The terminal processes of single and of pairs of identified photoreceptors in the eyes of the nudibranch mollusc Hermissenda crassicornis were studied by light and transmission electron microscopy after their somata were labelled by intracellular iontophoresis of horseradish peroxidase (HRP). The HRP spread from the somata into the axons and fine terminal processes within the neuropil of the cerebropleural ganglia. The photoreceptors ended in extensive secondary branches in the neuropil where previous electrophysiological studies had indicated the probable site of synaptic interactions between photoreceptors. Clear round vesicles (54-126 nm diameter) within labelled processes were similar to the vesicles found in the somata and axon hillocks. The terminal processes of pairs of type B photoreceptors contained different intensities of the HRP label. Uniform intensities of the HRP label were found in the terminal processes of single type B photoreceptors. These differences in intensity suggested that the terminal processes were from different type B photoreceptors. This finding suggests that the connections between type B photoreceptors are probably monosynaptic.", "contents": "Ultrastructure of photoreceptors in the eye of Hermissenda labelled with intracellular injections of horseradish peroxidase. The terminal processes of single and of pairs of identified photoreceptors in the eyes of the nudibranch mollusc Hermissenda crassicornis were studied by light and transmission electron microscopy after their somata were labelled by intracellular iontophoresis of horseradish peroxidase (HRP). The HRP spread from the somata into the axons and fine terminal processes within the neuropil of the cerebropleural ganglia. The photoreceptors ended in extensive secondary branches in the neuropil where previous electrophysiological studies had indicated the probable site of synaptic interactions between photoreceptors. Clear round vesicles (54-126 nm diameter) within labelled processes were similar to the vesicles found in the somata and axon hillocks. The terminal processes of pairs of type B photoreceptors contained different intensities of the HRP label. Uniform intensities of the HRP label were found in the terminal processes of single type B photoreceptors. These differences in intensity suggested that the terminal processes were from different type B photoreceptors. This finding suggests that the connections between type B photoreceptors are probably monosynaptic."}
{"id": "PMID:469573", "title": "Neurogenesis and neuron regeneration in the olfactory system of mammals. II. Degeneration and reconstitution of the olfactory sensory neurons after axotomy.", "content": "This report describes the retrograde degeneration affecting olfactory sensory neurons of rats after severance of their axons and illustrates the reconstitution of new neurons originating from stem cells located at the base of the olfactory neuroepithelium. Degeneration of the mature, axotomized neurons, signalled by an increased electron density of their cytoplasmic matrix and by the appearance of lipofuscin-like granules, can be detected in the neuroepithelium as early as 24 h after surgery and becomes conspicuous between the second and the third day. Degenerating neurons can be observed in decreasing number up to the tenth post-operative day. They are removed by macrophages which invade the epithelium. The reconstitution of new neurons begins to occur after eight days, when the stem cells undergo vigorous mitotic activity and differentiate into neurons. The morphology of the reconstituted neurons has been described in detail at different stages of their maturation. After 30 days, the olfactory epithelium appears similar to controls. On the basis of both morphological (in rats) and autoradiographic ( in mice) observations, the basal cells have been recognized as stem cells of the olfactory neurons.", "contents": "Neurogenesis and neuron regeneration in the olfactory system of mammals. II. Degeneration and reconstitution of the olfactory sensory neurons after axotomy. This report describes the retrograde degeneration affecting olfactory sensory neurons of rats after severance of their axons and illustrates the reconstitution of new neurons originating from stem cells located at the base of the olfactory neuroepithelium. Degeneration of the mature, axotomized neurons, signalled by an increased electron density of their cytoplasmic matrix and by the appearance of lipofuscin-like granules, can be detected in the neuroepithelium as early as 24 h after surgery and becomes conspicuous between the second and the third day. Degenerating neurons can be observed in decreasing number up to the tenth post-operative day. They are removed by macrophages which invade the epithelium. The reconstitution of new neurons begins to occur after eight days, when the stem cells undergo vigorous mitotic activity and differentiate into neurons. The morphology of the reconstituted neurons has been described in detail at different stages of their maturation. After 30 days, the olfactory epithelium appears similar to controls. On the basis of both morphological (in rats) and autoradiographic ( in mice) observations, the basal cells have been recognized as stem cells of the olfactory neurons."}
{"id": "PMID:469574", "title": "Ultrastructure of neurons in the auditory cortex of ageing rats: a morphometric study.", "content": "The cell bodies of pyramidal cells in layers II and V of rat auditory cortex were quantitatively examined in groups of rats 3, 6, 15, 23, 27 and 34-36 months of age. The mean diameters of cell bodies of both layer II and layer V neurons, as measured in 1 micron plastic sections, increased between 3 and 15 months of age, then decreased to a diameter that was less in the 36-month-old than in the 3-month-old rats. Morphometry of the nuclei of the cells was done by measuring nuclear area and nuclear envelope length directly on electron micrographs. In the layer II cells, neither parameter changed with advancing age. In the layer V cells, the mean nuclear area decreased significantly in the old animals and the mean envelope length increased. Point-counting techniques were applied to electron micrographs of cell bodies to determine the relative volume of selected organelles, inclusions and ground substance in the perikaryal cytoplasm. In this part of the study the chronological pattern of change in layer II and layer V pyramidal cells was similar. The relative volume of dense bodies increased linearly with advancing age, with a slightly more accelerated rate in layer II cells. The relative volume of ground substance remained essentially constant through 27 months, and then at 34-36 months decreased to 83% and 89% of the three-month level in layer V and layer II, respectively. The relative volume of the rough endoplasmic reticulum (RER) did not change significantly until after 15 months, at which time it began to occupy increasingly a larger fraction of the perikaryal cytoplasm. Finally, the relative volumes of mitochondria, multivesicular bodies and Golgi apparatus did not show clear trends of change during the 33-month period.", "contents": "Ultrastructure of neurons in the auditory cortex of ageing rats: a morphometric study. The cell bodies of pyramidal cells in layers II and V of rat auditory cortex were quantitatively examined in groups of rats 3, 6, 15, 23, 27 and 34-36 months of age. The mean diameters of cell bodies of both layer II and layer V neurons, as measured in 1 micron plastic sections, increased between 3 and 15 months of age, then decreased to a diameter that was less in the 36-month-old than in the 3-month-old rats. Morphometry of the nuclei of the cells was done by measuring nuclear area and nuclear envelope length directly on electron micrographs. In the layer II cells, neither parameter changed with advancing age. In the layer V cells, the mean nuclear area decreased significantly in the old animals and the mean envelope length increased. Point-counting techniques were applied to electron micrographs of cell bodies to determine the relative volume of selected organelles, inclusions and ground substance in the perikaryal cytoplasm. In this part of the study the chronological pattern of change in layer II and layer V pyramidal cells was similar. The relative volume of dense bodies increased linearly with advancing age, with a slightly more accelerated rate in layer II cells. The relative volume of ground substance remained essentially constant through 27 months, and then at 34-36 months decreased to 83% and 89% of the three-month level in layer V and layer II, respectively. The relative volume of the rough endoplasmic reticulum (RER) did not change significantly until after 15 months, at which time it began to occupy increasingly a larger fraction of the perikaryal cytoplasm. Finally, the relative volumes of mitochondria, multivesicular bodies and Golgi apparatus did not show clear trends of change during the 33-month period."}
{"id": "PMID:469575", "title": "The formation of gap and tight junctions between retinal pigment cells in cell cultures.", "content": "The reformation of the junctional complex of retinal pigment cells was studied after trypsin disaggregation and in vitro reaggregation. Control specimens show a zonula occludens (tight junction) with integrated gap junctions and very large macular gap junctions. Isolation after trypsination results in disaggregation of the large gap junctions and fragmentation of the tight junctions with disaggregation of their integrated gap junctions. After two to four days of incubation the restoration of the zonula occludens is complete. After approximately five days of incubation, large gap junctions are found with a patchy arrangement of particles similar to that seen in vivo.", "contents": "The formation of gap and tight junctions between retinal pigment cells in cell cultures. The reformation of the junctional complex of retinal pigment cells was studied after trypsin disaggregation and in vitro reaggregation. Control specimens show a zonula occludens (tight junction) with integrated gap junctions and very large macular gap junctions. Isolation after trypsination results in disaggregation of the large gap junctions and fragmentation of the tight junctions with disaggregation of their integrated gap junctions. After two to four days of incubation the restoration of the zonula occludens is complete. After approximately five days of incubation, large gap junctions are found with a patchy arrangement of particles similar to that seen in vivo."}
{"id": "PMID:469576", "title": "Development of synaptic ultrastructure at neuromuscular contacts in an amphibian cell culture system.", "content": "Cultures of dissociated myotomal muscle and spinal cord derived from embryos of Xenopus laevis were grown in the presence of curare in order to abolish neuromuscular activity and were examined by electron microscopy. In one-day-old cultures a few of the neuromuscular contacts already displayed several synaptic specializations including 500 A vesicles clustered against the axolemma, increased axolemmal densities, basal lamina in the cleft, an increased sarcolemmal density and subsarcolemmal filamentous material. Contacts with these specializations were observed more frequently in two and three-day-old cultures. Throughout the three-day culture period nerve fibres and neuromuscular contacts were devoid of Schwann cells. Isolated patches of basal lamina were relatively scarce and were usually accompanied by an increase in sarcolemmal density and subsarcolemmal filamentous material even in cultures in which spinal cord cells were not included. These observations indicate that the myotomal neuromuscular synapse differentiates in culture in much the same way as it does in vivo, that muscle contractions are not required for its differentiation, and that apparent postsynaptic specializations can develop in the absence of innervation.", "contents": "Development of synaptic ultrastructure at neuromuscular contacts in an amphibian cell culture system. Cultures of dissociated myotomal muscle and spinal cord derived from embryos of Xenopus laevis were grown in the presence of curare in order to abolish neuromuscular activity and were examined by electron microscopy. In one-day-old cultures a few of the neuromuscular contacts already displayed several synaptic specializations including 500 A vesicles clustered against the axolemma, increased axolemmal densities, basal lamina in the cleft, an increased sarcolemmal density and subsarcolemmal filamentous material. Contacts with these specializations were observed more frequently in two and three-day-old cultures. Throughout the three-day culture period nerve fibres and neuromuscular contacts were devoid of Schwann cells. Isolated patches of basal lamina were relatively scarce and were usually accompanied by an increase in sarcolemmal density and subsarcolemmal filamentous material even in cultures in which spinal cord cells were not included. These observations indicate that the myotomal neuromuscular synapse differentiates in culture in much the same way as it does in vivo, that muscle contractions are not required for its differentiation, and that apparent postsynaptic specializations can develop in the absence of innervation."}
{"id": "PMID:469577", "title": "Relation of cerebral blood flow to neurological status and outcome in head-injured patients.", "content": "Noninvasive studies of regional cerebral blood flow (CBF) were performed on 36 head-injured patients in varying degrees of coma, using the intravenous xenon-133 method. Serial examinations, averaging four per patient, were begun during the acute phase of illness and continued until death of recovery of normal consciousness. Comparison of the initial and final studies revealed that CBF declined to very low levels in all nine patients who died, and remained subnormal in a patient with persistent vegetative state. In contrast, 25 of 26 patients who recovered consciousness showed increases in blood flow. Because of the presence of both reduced and elevated blood flows on the initial study, CBF was not predictive of outcome. Absolute or relative hyperemia, observed in nine acute cases, was associated with either diffuse cerebral swelling (observed on computerized tomography) or recovery from systemic shock. Cerebral metabolic studies in hyperemic patients yielded a very low oxygen uptake and arteriovenous oxygen difference, indicating that the high blood flow was a true \"luxury perfusion.\" When instances of presumed luxury perfusion were excluded, CBF was positively correlated with level of consciousness, assessed on a four-point coma scale.", "contents": "Relation of cerebral blood flow to neurological status and outcome in head-injured patients. Noninvasive studies of regional cerebral blood flow (CBF) were performed on 36 head-injured patients in varying degrees of coma, using the intravenous xenon-133 method. Serial examinations, averaging four per patient, were begun during the acute phase of illness and continued until death of recovery of normal consciousness. Comparison of the initial and final studies revealed that CBF declined to very low levels in all nine patients who died, and remained subnormal in a patient with persistent vegetative state. In contrast, 25 of 26 patients who recovered consciousness showed increases in blood flow. Because of the presence of both reduced and elevated blood flows on the initial study, CBF was not predictive of outcome. Absolute or relative hyperemia, observed in nine acute cases, was associated with either diffuse cerebral swelling (observed on computerized tomography) or recovery from systemic shock. Cerebral metabolic studies in hyperemic patients yielded a very low oxygen uptake and arteriovenous oxygen difference, indicating that the high blood flow was a true \"luxury perfusion.\" When instances of presumed luxury perfusion were excluded, CBF was positively correlated with level of consciousness, assessed on a four-point coma scale."}
{"id": "PMID:469578", "title": "Failure of high-dose steroid therapy to influence intracranial pressure in patients with severe head injury.", "content": "While corticosteroids in standard neurosurgical dosage do not appear to influence recovery from severe head injury or elevated intracranial pressure (ICP), some reports claim that a much higher dose is effective. A resultant hypothesis is that an abrupt increase in corticosteroid dosage in patients with severe head injury should cause a detectable reduction in ICP and in cerebral elastance within 48 hours. To test this hypothesis 20 consecutive patients with severe head injury, 12 of whom had had surgical decompression of mass lesions, were studied. All patients were artificially ventilated, and had continuous monitoring of ICP and intermittent testing of elastance by measurement of the volume-pressure response (VPR). For the first 12 hours after admission, patients received methylprednisolone, 40 mg every 6 hours. The dose was then increased abruptly by giving a single dose of 2 gm and 500 mg every 6 hours for the next 24 hours, then tapering rapidly. No significant change in ICP or VPR could be detected after 24 or 48 hours of high-dose steroid therapy. Of the 20 patients, 50% had good recovery or were moderately disabled, 15% were severely disabled or vegetative, and 35% had died. The course of ICP and the outcomes in these patients were not significantly different from those observed in a previous group of 262 patients managed in the same way for the high-dose regimen. There was, however, high incidence of gastric hemorrhage (50%) and of hyperglycemia with glucosuria (85%) in these 20 patients. These negative results in patients with head injury stand in marked contrast to our experience of the benefit of methylprednisolone in patients with brain tumors.", "contents": "Failure of high-dose steroid therapy to influence intracranial pressure in patients with severe head injury. While corticosteroids in standard neurosurgical dosage do not appear to influence recovery from severe head injury or elevated intracranial pressure (ICP), some reports claim that a much higher dose is effective. A resultant hypothesis is that an abrupt increase in corticosteroid dosage in patients with severe head injury should cause a detectable reduction in ICP and in cerebral elastance within 48 hours. To test this hypothesis 20 consecutive patients with severe head injury, 12 of whom had had surgical decompression of mass lesions, were studied. All patients were artificially ventilated, and had continuous monitoring of ICP and intermittent testing of elastance by measurement of the volume-pressure response (VPR). For the first 12 hours after admission, patients received methylprednisolone, 40 mg every 6 hours. The dose was then increased abruptly by giving a single dose of 2 gm and 500 mg every 6 hours for the next 24 hours, then tapering rapidly. No significant change in ICP or VPR could be detected after 24 or 48 hours of high-dose steroid therapy. Of the 20 patients, 50% had good recovery or were moderately disabled, 15% were severely disabled or vegetative, and 35% had died. The course of ICP and the outcomes in these patients were not significantly different from those observed in a previous group of 262 patients managed in the same way for the high-dose regimen. There was, however, high incidence of gastric hemorrhage (50%) and of hyperglycemia with glucosuria (85%) in these 20 patients. These negative results in patients with head injury stand in marked contrast to our experience of the benefit of methylprednisolone in patients with brain tumors."}
{"id": "PMID:469579", "title": "Cerebrospinal fluid cytology after subarachnoid hemorrhage.", "content": "A method is described which has been found capable of detecting subarachnoid hemorrhage (SAH) up to 15 to 17 weeks after its occurrence. The episode of SAH was confirmed by bloody and/or xanthochromic cerebrospinal fluid (CSF) at the time of SAH onset. In this study, 47 samples of lumbar CSF from diagnostically confirmed SAH patients were used. The CSF cells were collected onto slides and stained with May-Gruenwald-Giemsa or Perl's reagent. Iron-positive cells were detected at 1 week, increased by 4 to 6 weeks to 8.5% of total nucleated cells, and decreased to 1% by 15 to 17 weeks. All 27 samples obtained at 2 to 9 weeks after SAH showed iron-positive cells. No iron-positive cells (false-negative samples) were noted in 25% (one of four) of samples obtained during the first week, and in 33% (one of three) of samples obtained 10 to 12 weeks and 15 to 17 weeks after SAH. Of the total samples (37) obtained within 17 weeks after SAH, 8.1% (three of 37) were false negative. No iron-positive cells were detected in samples obtained later than 21 weeks after the SAH episode (10 samples).", "contents": "Cerebrospinal fluid cytology after subarachnoid hemorrhage. A method is described which has been found capable of detecting subarachnoid hemorrhage (SAH) up to 15 to 17 weeks after its occurrence. The episode of SAH was confirmed by bloody and/or xanthochromic cerebrospinal fluid (CSF) at the time of SAH onset. In this study, 47 samples of lumbar CSF from diagnostically confirmed SAH patients were used. The CSF cells were collected onto slides and stained with May-Gruenwald-Giemsa or Perl's reagent. Iron-positive cells were detected at 1 week, increased by 4 to 6 weeks to 8.5% of total nucleated cells, and decreased to 1% by 15 to 17 weeks. All 27 samples obtained at 2 to 9 weeks after SAH showed iron-positive cells. No iron-positive cells (false-negative samples) were noted in 25% (one of four) of samples obtained during the first week, and in 33% (one of three) of samples obtained 10 to 12 weeks and 15 to 17 weeks after SAH. Of the total samples (37) obtained within 17 weeks after SAH, 8.1% (three of 37) were false negative. No iron-positive cells were detected in samples obtained later than 21 weeks after the SAH episode (10 samples)."}
{"id": "PMID:469580", "title": "New data on diastematomyelia.", "content": "Five cases of diastematomyelia are described and illustrated. These and a further 60 cases from the literature are analyzed. The data corroborate the opinion that early diagnosis and treatment result in a better prognosis.", "contents": "New data on diastematomyelia. Five cases of diastematomyelia are described and illustrated. These and a further 60 cases from the literature are analyzed. The data corroborate the opinion that early diagnosis and treatment result in a better prognosis."}
{"id": "PMID:469581", "title": "Cervical myelopathy caused by developmental stenosis of the spinal canal.", "content": "The authors present six patients with myelopathy caused by developmental stenosis of the cervical spine. Hyperextension injuries precipitated the onset of symptoms in two patients, aged 19 and 20 years. In four, 41 to 69 years of age, symptoms were gradual in onset, progressing to severe disability. X-ray films revealed narrowing of the dorsoventral diameter of the spinal canal to as little as 1.0 cm. The myelograms showed widening of the cord in the transverse plane strongly suggestive of an intramedullary tumor. A unique finding was maldevelopment with flattening of the neural arch often hidden by the posterior portions of the articular facets when seen in the lateral views. These patients showed no significant evidence of spondylosis, arthrosis, or any of the structural stigmata usually observed in cervical spondylotic myelopathy. When indicated, decompressive laminectomy is the treatment of choice.", "contents": "Cervical myelopathy caused by developmental stenosis of the spinal canal. The authors present six patients with myelopathy caused by developmental stenosis of the cervical spine. Hyperextension injuries precipitated the onset of symptoms in two patients, aged 19 and 20 years. In four, 41 to 69 years of age, symptoms were gradual in onset, progressing to severe disability. X-ray films revealed narrowing of the dorsoventral diameter of the spinal canal to as little as 1.0 cm. The myelograms showed widening of the cord in the transverse plane strongly suggestive of an intramedullary tumor. A unique finding was maldevelopment with flattening of the neural arch often hidden by the posterior portions of the articular facets when seen in the lateral views. These patients showed no significant evidence of spondylosis, arthrosis, or any of the structural stigmata usually observed in cervical spondylotic myelopathy. When indicated, decompressive laminectomy is the treatment of choice."}
{"id": "PMID:469582", "title": "Traumatic asphyxia: an unusual cause of traumatic coma and paraplegia. Case report.", "content": "An unusual case of traumatic asphyxia with cerebral and spinal cord symptoms is presented. A survey of the literature indicates that a primary cord lesion is very rare as a consequence of such a trauma. The possible pathogenetic mechanisms of these neurological manifestations are discussed.", "contents": "Traumatic asphyxia: an unusual cause of traumatic coma and paraplegia. Case report. An unusual case of traumatic asphyxia with cerebral and spinal cord symptoms is presented. A survey of the literature indicates that a primary cord lesion is very rare as a consequence of such a trauma. The possible pathogenetic mechanisms of these neurological manifestations are discussed."}
{"id": "PMID:469583", "title": "Total removal of a parasagittal meningioma of the posterior third of the sagittal sinus and its repair by autogenous vein graft. Case report.", "content": "Total removal is reported of a parasaggital meningioma that invaded the lateral wall and lateral recess of the posterior third of the superior sagittal sinus. The operation was performed by using a silicone rubber non-collapsible tube with an inflatable balloon cuff at each end for the bypass of the sinus blood, and entailed repair of the defect in the sinus wall by a saphenous vein graft. The clinical results and angiographic findings are presented.", "contents": "Total removal of a parasagittal meningioma of the posterior third of the sagittal sinus and its repair by autogenous vein graft. Case report. Total removal is reported of a parasaggital meningioma that invaded the lateral wall and lateral recess of the posterior third of the superior sagittal sinus. The operation was performed by using a silicone rubber non-collapsible tube with an inflatable balloon cuff at each end for the bypass of the sinus blood, and entailed repair of the defect in the sinus wall by a saphenous vein graft. The clinical results and angiographic findings are presented."}
{"id": "PMID:469584", "title": "Extraspinal ependymomas. Report of three cases.", "content": "Spinal ependymomas may rarely arise from heterotopic ependymal cell clusters and thus occur in an extraspinal location. Presentation of three cases and a review of the literature reveal that these tumors have characteristic radiographic and clinical features. They occur mainly in patients in the third decade of life, and present either in the soft tissue posterior to the sacrum or in the pelvis. In the case of posterior tumors, the patient exhibits a mass which is usually mistaken for a pilonidal cyst. Patients whose tumor is pelvic in location present with sphincter disturbances or dysfunction of the sacral nerve roots. Conventional and computerized tomographic studies will reveal erosion of the sacrum. Myelography will demonstrate an extradural mass indenting the thecal sac from below. The protein in the cerebrospinal fluid will be normal. A combined posterior and anterior approach with the goal of complete tumor removal is the procedure of choice. If this is not feasible, then radiation therapy should be employed. Because of the increased incidence of systemic metastases, the average postoperative survival is approximately 10 years.", "contents": "Extraspinal ependymomas. Report of three cases. Spinal ependymomas may rarely arise from heterotopic ependymal cell clusters and thus occur in an extraspinal location. Presentation of three cases and a review of the literature reveal that these tumors have characteristic radiographic and clinical features. They occur mainly in patients in the third decade of life, and present either in the soft tissue posterior to the sacrum or in the pelvis. In the case of posterior tumors, the patient exhibits a mass which is usually mistaken for a pilonidal cyst. Patients whose tumor is pelvic in location present with sphincter disturbances or dysfunction of the sacral nerve roots. Conventional and computerized tomographic studies will reveal erosion of the sacrum. Myelography will demonstrate an extradural mass indenting the thecal sac from below. The protein in the cerebrospinal fluid will be normal. A combined posterior and anterior approach with the goal of complete tumor removal is the procedure of choice. If this is not feasible, then radiation therapy should be employed. Because of the increased incidence of systemic metastases, the average postoperative survival is approximately 10 years."}
{"id": "PMID:469585", "title": "Myelocystocele with cerebellar heterotopia. Case report.", "content": "The authors describe a case with well differentiated cerebellar tissue contained in a hydromyelic dilation of the spinal cord, that is, myelocystocele, in connection with a cervicothoracic meningocele. The embryology is discussed.", "contents": "Myelocystocele with cerebellar heterotopia. Case report. The authors describe a case with well differentiated cerebellar tissue contained in a hydromyelic dilation of the spinal cord, that is, myelocystocele, in connection with a cervicothoracic meningocele. The embryology is discussed."}
{"id": "PMID:469586", "title": "Sphenoethmoidal meningoencephalocele associated with agenesis of corpus callosum and median cleft lip and palate. Case report.", "content": "A case of sphenoethmoidal meningoencephalocele associated with agenesis of the corpus callosum and median cleft lip and palate in a baby boy is reported. Two other cases have been reported previously presenting exactly the same findings. It is possible that these malformations constitute a distinct type of congenital anomaly.", "contents": "Sphenoethmoidal meningoencephalocele associated with agenesis of corpus callosum and median cleft lip and palate. Case report. A case of sphenoethmoidal meningoencephalocele associated with agenesis of the corpus callosum and median cleft lip and palate in a baby boy is reported. Two other cases have been reported previously presenting exactly the same findings. It is possible that these malformations constitute a distinct type of congenital anomaly."}
{"id": "PMID:469587", "title": "Sphenoidal pneumosinus dilatans with bilateral optic nerve meningiomas. Case report.", "content": "The authors report a case of bilateral progressive visual loss in a patient with a radiological diagnosis of sphenoidal pneumosinus dilatans. At craniotomy, bilaterally symmetrical meningiomas of the optic nerve sheaths were found at the intracranial optic foramina. Reported cases of sphenoidal pneumosinus dilatans as well as bilateral meningiomas of the optic nerve sheaths are reviewed. The possible mechanisms of visual loss in pneumosinus dilatans and the management of nerve sheath and planum sphenoidale meningiomas are discussed.", "contents": "Sphenoidal pneumosinus dilatans with bilateral optic nerve meningiomas. Case report. The authors report a case of bilateral progressive visual loss in a patient with a radiological diagnosis of sphenoidal pneumosinus dilatans. At craniotomy, bilaterally symmetrical meningiomas of the optic nerve sheaths were found at the intracranial optic foramina. Reported cases of sphenoidal pneumosinus dilatans as well as bilateral meningiomas of the optic nerve sheaths are reviewed. The possible mechanisms of visual loss in pneumosinus dilatans and the management of nerve sheath and planum sphenoidale meningiomas are discussed."}
{"id": "PMID:469588", "title": "Primary cerebellar nocardiosis. Report of two cases.", "content": "The authors describe two cases of primary cerebellar nocardiosis. Confinement of Nocardia abscesses to a localized, accessible portion of the central nervous system should favor surgical cure of this aggressive and often fatal disease. In our two cases multilocularity, tenuous encapsulation, and proximity to the brain stem prevented complete primary excision. Nevertheless, the infections were effectively treated by intensive postoperative antibiotic therapy and, in one case, a second operation to excise the residual abscess. The need for the latter was suggested by the results of sequential computerized tomographic brain scans that were used to monitor the response to antibiotic therapy. In the absence of any apparent extracranial focus of infection and any overt condition that might impair immunological competence, nocardiosis is likely to be omitted from the preoperative differential diagnosis of a posterior fossa space-occupying lesion.", "contents": "Primary cerebellar nocardiosis. Report of two cases. The authors describe two cases of primary cerebellar nocardiosis. Confinement of Nocardia abscesses to a localized, accessible portion of the central nervous system should favor surgical cure of this aggressive and often fatal disease. In our two cases multilocularity, tenuous encapsulation, and proximity to the brain stem prevented complete primary excision. Nevertheless, the infections were effectively treated by intensive postoperative antibiotic therapy and, in one case, a second operation to excise the residual abscess. The need for the latter was suggested by the results of sequential computerized tomographic brain scans that were used to monitor the response to antibiotic therapy. In the absence of any apparent extracranial focus of infection and any overt condition that might impair immunological competence, nocardiosis is likely to be omitted from the preoperative differential diagnosis of a posterior fossa space-occupying lesion."}
{"id": "PMID:469594", "title": "An association of upper respiratory cancer with exposure to diethyl sulfate.", "content": "A morbidity and mortality study of workers at an alcohol manufacturing plant which included several weak acid isopropyl alcohol units and a strong acid ethanol unit is described. An excess mortality of upper respiratory cancer was found and associated with work on the strong acid ethanol unit. The strong acid ethanol process used resulted in high concentrations of diethyl sulfate, which has been shown to be carcinogenic in animals, and the unit, which closed on 1975, had significant opportunities for worker exposure to diethyl sulfate. These facts, plus previous reports of excess upper respiratory cancer on strong acid isopropyl alcohol units with similarly high concentrations of the animal carcinogen diisopropyl sulfate, lead to the tentative conclusion that diethyl sulfate was primarily responsible for the ethanol unit cancer cases. In the modern weak acid isopropyl alcohol plants, where only trace amounts of diisopropyl sulfate are present and exposures are much lower, the problems found on the old strong acid units do not exist.", "contents": "An association of upper respiratory cancer with exposure to diethyl sulfate. A morbidity and mortality study of workers at an alcohol manufacturing plant which included several weak acid isopropyl alcohol units and a strong acid ethanol unit is described. An excess mortality of upper respiratory cancer was found and associated with work on the strong acid ethanol unit. The strong acid ethanol process used resulted in high concentrations of diethyl sulfate, which has been shown to be carcinogenic in animals, and the unit, which closed on 1975, had significant opportunities for worker exposure to diethyl sulfate. These facts, plus previous reports of excess upper respiratory cancer on strong acid isopropyl alcohol units with similarly high concentrations of the animal carcinogen diisopropyl sulfate, lead to the tentative conclusion that diethyl sulfate was primarily responsible for the ethanol unit cancer cases. In the modern weak acid isopropyl alcohol plants, where only trace amounts of diisopropyl sulfate are present and exposures are much lower, the problems found on the old strong acid units do not exist."}
{"id": "PMID:469595", "title": "Nonsmoking grain handlers in Saskatchewan: airways reactivity and allergic status.", "content": "This study was carried out to determine whether respiratory disease in Saskatchewan grainhandlers was or was not due to hypersensitivity to grain dust. Sixty nonsmoking grainhandlers, half of whom had low FEV1/FVC ratios and half of whom had high, were studied. The findings in grainhandlers were compared with those in 30 age-matched, nonsmoking teachers. In the group of grainhandlers with low FEV1/FVC ratios, one had developed asthma associated with exposure to grain dust. The majority were healthy, had FEV1/FVC ratios of 70% or greater, and had less evidence of allergic respiratory disease than had age-matched teachers. The studies suggest that non-atopic, nonsmoking men can work in grain elevators without developing respiratory disability, though they may develop a small but significant increase in nonspecific bronchial reactivity to histamine.", "contents": "Nonsmoking grain handlers in Saskatchewan: airways reactivity and allergic status. This study was carried out to determine whether respiratory disease in Saskatchewan grainhandlers was or was not due to hypersensitivity to grain dust. Sixty nonsmoking grainhandlers, half of whom had low FEV1/FVC ratios and half of whom had high, were studied. The findings in grainhandlers were compared with those in 30 age-matched, nonsmoking teachers. In the group of grainhandlers with low FEV1/FVC ratios, one had developed asthma associated with exposure to grain dust. The majority were healthy, had FEV1/FVC ratios of 70% or greater, and had less evidence of allergic respiratory disease than had age-matched teachers. The studies suggest that non-atopic, nonsmoking men can work in grain elevators without developing respiratory disability, though they may develop a small but significant increase in nonspecific bronchial reactivity to histamine."}
{"id": "PMID:469596", "title": "Lung cancer mortality in aluminum reduction plant workers.", "content": "The lung cancer mortaltiy of 5406 men (cohort 1) employed at one aluminum smelter on January 1, 1950, and of 485 men employed at a second plant (cohort 2) on January 1, 1951, is reported. For each man, the total number of years of exposure to tars, number of years since first exposure to tars and an index of exposure to tars expressed in tar-years were calculated. More than 99% of the men in the first cohort and just less than 98% of the men in the second cohort were traced. As of December 31, 1973, 1070 men in cohort 1 had died and death certificates were obtained for 990 men (92.5%). As of December 31, 1973, 64 men in cohort 2 had died and death certificates were obtained for 58 (91%). There were 84 deaths from lung cancer in cohort 1 and 11 in cohort 2. The results showed that the mortality from lung cancer of that portion of the cohorts 1 and 2 combined who had ever been exposed to tars was similar to that of workers never exposed to tars. The mortality from lung cancer of men in cohort 1 was greater than that expected at Quebec provincial rates, but this was probably due to slightly increased lung cancer mortality in the communities serving the industries. Although the total number of cases in cohort 2 was small tuld not be explained on community experience. There was a definite dose-response relationship between lung cancer mortality and tar-years and years of exposure. The standardized mortality ratio for persons exposed for more than 21 years to the higher levels of tars was 2.3 times that of persons not exposed to tars. Although smoking may still be a factor, the evidence suggests that the increased risk of lung cancer is related to employment in definite tar-exposed occupations.", "contents": "Lung cancer mortality in aluminum reduction plant workers. The lung cancer mortaltiy of 5406 men (cohort 1) employed at one aluminum smelter on January 1, 1950, and of 485 men employed at a second plant (cohort 2) on January 1, 1951, is reported. For each man, the total number of years of exposure to tars, number of years since first exposure to tars and an index of exposure to tars expressed in tar-years were calculated. More than 99% of the men in the first cohort and just less than 98% of the men in the second cohort were traced. As of December 31, 1973, 1070 men in cohort 1 had died and death certificates were obtained for 990 men (92.5%). As of December 31, 1973, 64 men in cohort 2 had died and death certificates were obtained for 58 (91%). There were 84 deaths from lung cancer in cohort 1 and 11 in cohort 2. The results showed that the mortality from lung cancer of that portion of the cohorts 1 and 2 combined who had ever been exposed to tars was similar to that of workers never exposed to tars. The mortality from lung cancer of men in cohort 1 was greater than that expected at Quebec provincial rates, but this was probably due to slightly increased lung cancer mortality in the communities serving the industries. Although the total number of cases in cohort 2 was small tuld not be explained on community experience. There was a definite dose-response relationship between lung cancer mortality and tar-years and years of exposure. The standardized mortality ratio for persons exposed for more than 21 years to the higher levels of tars was 2.3 times that of persons not exposed to tars. Although smoking may still be a factor, the evidence suggests that the increased risk of lung cancer is related to employment in definite tar-exposed occupations."}
{"id": "PMID:469597", "title": "Sulfur dioxide exposure in a smelter. III. Acute effects and sputum cytology.", "content": "Although acute effects from exposure to SO2 have frequently been observed at low levels in acute exposure experimental studies, it was not known whether or not such effects occur among workers chronically exposed at ranges of 0.3 to 4 ppm of SO2. Measurements of FVC, FEV1, FEF50, FEF75 and FEF50-75, and closing volume were made before and after the workshift for copper smelter workers and controls. Sputum samples for cytological examination were collected. Mean FEV1 and FVC were significantly more smelter workers had a decrease in FEV1 and FEF50 during the day than did controls. More of the smelter workers felt \"chest tightness.\" No change in closing volumes was seen. Smelter workers tended to have a higher percentage of sputum samples with moderate and marked atypia than did controls but the difference was not statistically significant.", "contents": "Sulfur dioxide exposure in a smelter. III. Acute effects and sputum cytology. Although acute effects from exposure to SO2 have frequently been observed at low levels in acute exposure experimental studies, it was not known whether or not such effects occur among workers chronically exposed at ranges of 0.3 to 4 ppm of SO2. Measurements of FVC, FEV1, FEF50, FEF75 and FEF50-75, and closing volume were made before and after the workshift for copper smelter workers and controls. Sputum samples for cytological examination were collected. Mean FEV1 and FVC were significantly more smelter workers had a decrease in FEV1 and FEF50 during the day than did controls. More of the smelter workers felt \"chest tightness.\" No change in closing volumes was seen. Smelter workers tended to have a higher percentage of sputum samples with moderate and marked atypia than did controls but the difference was not statistically significant."}
{"id": "PMID:469598", "title": "Industrial accidents and medication. Is there a relationship?", "content": "Although health workers are continually concerned about the effects of various medications on the public and especially on employees at work, there have been almost no studies reported which have indicated any relationship between medication taken and industrial accidents. A series of 115 consecutive employees reporting accidents to the DRG Health Center were interviewed as to medication taken in the previous 24 hours. A control group of another 115 employees were also interviewed. We found no difference in the two groups related to medication taken and thus there does not appear to be a direct relationship between medication ingestion and accident rate within this industry.", "contents": "Industrial accidents and medication. Is there a relationship? Although health workers are continually concerned about the effects of various medications on the public and especially on employees at work, there have been almost no studies reported which have indicated any relationship between medication taken and industrial accidents. A series of 115 consecutive employees reporting accidents to the DRG Health Center were interviewed as to medication taken in the previous 24 hours. A control group of another 115 employees were also interviewed. We found no difference in the two groups related to medication taken and thus there does not appear to be a direct relationship between medication ingestion and accident rate within this industry."}
{"id": "PMID:469599", "title": "A mortality study of oil refinery workers.", "content": "Survival status of 1205 men employed for more than five years in a Canadian oil refinery in East Montreal, from 1928 through 1976, was assessed and death certificates were reviewed. Expected numbers of deaths were estimated based upon age- and cause-specific death rates for the Province of Quebec applied to person-years at work. Oil refinery workers showed a standard mortality ratio lower than expected for all causes of death (SMR = 78.43). Three cancers of the brain were found among young people who died less than 20 years since start of exposure. This was statistically higher than expected. Cancers of the digestive system, though not significantly higher than expected, remained suspect of being associated with work. There is a need to expand this research to other oil refinery workers.", "contents": "A mortality study of oil refinery workers. Survival status of 1205 men employed for more than five years in a Canadian oil refinery in East Montreal, from 1928 through 1976, was assessed and death certificates were reviewed. Expected numbers of deaths were estimated based upon age- and cause-specific death rates for the Province of Quebec applied to person-years at work. Oil refinery workers showed a standard mortality ratio lower than expected for all causes of death (SMR = 78.43). Three cancers of the brain were found among young people who died less than 20 years since start of exposure. This was statistically higher than expected. Cancers of the digestive system, though not significantly higher than expected, remained suspect of being associated with work. There is a need to expand this research to other oil refinery workers."}
{"id": "PMID:469602", "title": "Racial differences in lung function: search for proportional relationships.", "content": "A comparison of lung function in Caucasian and East Indian woodworkers revealed marked differences in FEV, FVC, AND MMF in relation to predicted age- and height-specific values of these variables reported in the literature. These findings were not attributable to differences in smoking habits, frequency of respiratory disease, or employment characteristics. Rather, they suggested that the standard values employed were inappropriate for East Indians. An attempt was made to confirm previously reported suggestions of consistent proportional differences in lung function between racial groups which would permit the calculation of a single correction  factor. Evidence of a consistent proportional relationship was found for FEV over a wide range of heights and for FVC over a limited range of heights. However, no such relationship was found for MMF.", "contents": "Racial differences in lung function: search for proportional relationships. A comparison of lung function in Caucasian and East Indian woodworkers revealed marked differences in FEV, FVC, AND MMF in relation to predicted age- and height-specific values of these variables reported in the literature. These findings were not attributable to differences in smoking habits, frequency of respiratory disease, or employment characteristics. Rather, they suggested that the standard values employed were inappropriate for East Indians. An attempt was made to confirm previously reported suggestions of consistent proportional differences in lung function between racial groups which would permit the calculation of a single correction  factor. Evidence of a consistent proportional relationship was found for FEV over a wide range of heights and for FVC over a limited range of heights. However, no such relationship was found for MMF."}
{"id": "PMID:469603", "title": "The crossed straight leg raising test: a diagnostic sign of herniated disc.", "content": "Increased sciatica on raising the opposite or \"well\" leg, the crossed straight leg raising (XSLR) sign, is associated with a herniated lumbar disc in 97% of patients. Although XSLR predicts poor response to conservative management, the results of laminectomy are usually good, with 91% of patients returning to work. Myelography is unnecessary for the diagnosis of disc herniation in patients with XSLR. It is possible for patients with this sign to have a normal myelogram, but 90% of them will prove nevertheless to have a herniated disc.", "contents": "The crossed straight leg raising test: a diagnostic sign of herniated disc. Increased sciatica on raising the opposite or \"well\" leg, the crossed straight leg raising (XSLR) sign, is associated with a herniated lumbar disc in 97% of patients. Although XSLR predicts poor response to conservative management, the results of laminectomy are usually good, with 91% of patients returning to work. Myelography is unnecessary for the diagnosis of disc herniation in patients with XSLR. It is possible for patients with this sign to have a normal myelogram, but 90% of them will prove nevertheless to have a herniated disc."}
{"id": "PMID:469604", "title": "Medical effects of combined industrial exposures.", "content": "The problem of combined workplace exposures has been long recognized but rarely written about. This article explores some of the problems of approaching and defining-combined exposures, reviews several categories of combined exposures, and emphasizes a practical preventive medicine approach to combined exposures. It is hoped that this article will stimulate other papers in this area.", "contents": "Medical effects of combined industrial exposures. The problem of combined workplace exposures has been long recognized but rarely written about. This article explores some of the problems of approaching and defining-combined exposures, reviews several categories of combined exposures, and emphasizes a practical preventive medicine approach to combined exposures. It is hoped that this article will stimulate other papers in this area."}
{"id": "PMID:469606", "title": "Argyria: clinical implications of exposure to silver nitrate and silver oxide.", "content": "This article reports the clinical findings in a work force of 30 individuals who were exposed to silver nitrate and silver oxide. Six individuals had argyria and 20 had argyrosis (deposition of silver in the eye). Measurements of blood silver levels were included as part of the examination. The results of this examination generally support the benign nature of argyria, although the question of silver causing a decrement in kidney function and night vision is not settled. Periodic slit lamp examinations as well as monitoring of silver air concentrations are necessary to assure that engineering controls are actually limiting worker exposure to silver.", "contents": "Argyria: clinical implications of exposure to silver nitrate and silver oxide. This article reports the clinical findings in a work force of 30 individuals who were exposed to silver nitrate and silver oxide. Six individuals had argyria and 20 had argyrosis (deposition of silver in the eye). Measurements of blood silver levels were included as part of the examination. The results of this examination generally support the benign nature of argyria, although the question of silver causing a decrement in kidney function and night vision is not settled. Periodic slit lamp examinations as well as monitoring of silver air concentrations are necessary to assure that engineering controls are actually limiting worker exposure to silver."}
{"id": "PMID:469607", "title": "Scleroderma simulating vinyl chloride disease.", "content": "Progressive systemic sclerosis, especially prior to distinctive internal organ involvement can be difficult to distinguish from vinyl chloride disease. A 50-year-old male developed classic scleroderma while working with polyvinyl chloride. Early in his disease he displayed a number of features common to both disorders, including acroosteolysis, sclerodactyly, polygammopathy and circulating immune complexes. Characteristic visceral involvement was the cardinal feature which distringuished his disease from the pseudoscleroderma of vinyl chloride disease.", "contents": "Scleroderma simulating vinyl chloride disease. Progressive systemic sclerosis, especially prior to distinctive internal organ involvement can be difficult to distinguish from vinyl chloride disease. A 50-year-old male developed classic scleroderma while working with polyvinyl chloride. Early in his disease he displayed a number of features common to both disorders, including acroosteolysis, sclerodactyly, polygammopathy and circulating immune complexes. Characteristic visceral involvement was the cardinal feature which distringuished his disease from the pseudoscleroderma of vinyl chloride disease."}
{"id": "PMID:469609", "title": "Strategies for increasing employees' level of exercise and physical fitness.", "content": "Many organizations are attempting to increase the physical fitness levels of their employees to help stem the rapidly-increasing employer health costs. Using basic economic concepts and models of human health behaviors, this study finds that the time and effort costs of exercise may exceed the present value of the economic benefits of improved physical fitness. These high costs help explain the small incidence of consistent adult exercise and the lack of in-house, on-work-time employer exercise programs. The study concludes that while health education campaigns may be useful, employers must find ways to reduce the time and effort costs of exercise by making  it a low-cost byproduct of more immediately desired outputs or goals.", "contents": "Strategies for increasing employees' level of exercise and physical fitness. Many organizations are attempting to increase the physical fitness levels of their employees to help stem the rapidly-increasing employer health costs. Using basic economic concepts and models of human health behaviors, this study finds that the time and effort costs of exercise may exceed the present value of the economic benefits of improved physical fitness. These high costs help explain the small incidence of consistent adult exercise and the lack of in-house, on-work-time employer exercise programs. The study concludes that while health education campaigns may be useful, employers must find ways to reduce the time and effort costs of exercise by making  it a low-cost byproduct of more immediately desired outputs or goals."}
{"id": "PMID:469610", "title": "Utilization of the preemployment health examination in a university staff health service.", "content": "This study assessed the utilization of a mandatory preemployment health examination (PEHE) for 507 new full-time university staff in 1976. A random sample of 126 new employees was interviewed by telephone. Of the 108(86%) who completed the interview, 80 (74%) had been examined by the staff health service. Notification to take the exam, a reminder, and the employee's perception of its importance were highly associated (p less than 0.0001) with compliance. Only 30% of the food handlers and maintenance personnel ahd their PEHE before employment started; the proportion was similar for all new employees (29%). Therefore, although this staff health service was moderately successful in getting most new employees to have a PEHE, if the value of the examination is to be enhances, more attention must be paid to notifying and reminding all prospective employees. Special consideration is needed to ensure that those in potentially high-risk jobs are examined prior to employment. The role of the university administration in improving compliance is emphasized.", "contents": "Utilization of the preemployment health examination in a university staff health service. This study assessed the utilization of a mandatory preemployment health examination (PEHE) for 507 new full-time university staff in 1976. A random sample of 126 new employees was interviewed by telephone. Of the 108(86%) who completed the interview, 80 (74%) had been examined by the staff health service. Notification to take the exam, a reminder, and the employee's perception of its importance were highly associated (p less than 0.0001) with compliance. Only 30% of the food handlers and maintenance personnel ahd their PEHE before employment started; the proportion was similar for all new employees (29%). Therefore, although this staff health service was moderately successful in getting most new employees to have a PEHE, if the value of the examination is to be enhances, more attention must be paid to notifying and reminding all prospective employees. Special consideration is needed to ensure that those in potentially high-risk jobs are examined prior to employment. The role of the university administration in improving compliance is emphasized."}
{"id": "PMID:469611", "title": "The use of medical records by adjudicators in processing Social Security disability insurance benefit claims.", "content": "The Social Security Administration contracts with state agencies to process disability claims filed under the Social Security Act. These state agencies employ physicians and lay adjudicators who secure medical reports from physicians, hospitals and clinics, and use these reports in awarding or denying disability benefits. This nationwide study showed that well over half of the lay adjudicators are involved in developing and interpreting medical information independently. This suggests that appropriate hiring practices, adequate training and certification procedures should be instituted to ensure that the lay disability adjudicators are able to conduct their job efficiently and correctly.", "contents": "The use of medical records by adjudicators in processing Social Security disability insurance benefit claims. The Social Security Administration contracts with state agencies to process disability claims filed under the Social Security Act. These state agencies employ physicians and lay adjudicators who secure medical reports from physicians, hospitals and clinics, and use these reports in awarding or denying disability benefits. This nationwide study showed that well over half of the lay adjudicators are involved in developing and interpreting medical information independently. This suggests that appropriate hiring practices, adequate training and certification procedures should be instituted to ensure that the lay disability adjudicators are able to conduct their job efficiently and correctly."}
{"id": "PMID:469612", "title": "Mortality in aluminum reduction plant workers.", "content": "Mortality in workers at a northwestern United States prebake-type aluminum reduction plant was examined in a historical prospective (cohort) study. Although the standardized mortality ratio (SMR) for all causes of death was low (86), lung cancer (SMR 117), pancreatic cancer (SMR 180), lymphatic and hematopoietic cancers (SMR 184), fatal benign tumors of the brain (SMR 391), and pulmonary emphysema (SMR 204) showed excess deaths. Analysis of mortality by job-exposure category, duration of employment and latency suggests that some of the lymphatic and hematopoietic cancers (especially malignant lymphoma), lung cancers and pulmonary emphysema may be of occupational origin in this worker population.", "contents": "Mortality in aluminum reduction plant workers. Mortality in workers at a northwestern United States prebake-type aluminum reduction plant was examined in a historical prospective (cohort) study. Although the standardized mortality ratio (SMR) for all causes of death was low (86), lung cancer (SMR 117), pancreatic cancer (SMR 180), lymphatic and hematopoietic cancers (SMR 184), fatal benign tumors of the brain (SMR 391), and pulmonary emphysema (SMR 204) showed excess deaths. Analysis of mortality by job-exposure category, duration of employment and latency suggests that some of the lymphatic and hematopoietic cancers (especially malignant lymphoma), lung cancers and pulmonary emphysema may be of occupational origin in this worker population."}
{"id": "PMID:469613", "title": "Carpal tunnel syndrome and selected personal attributes.", "content": "Carpal tunnel syndrome has been reported to occur between two and ten times as frequently in women as in men. It also has been reported that this syndrome is often associated with the performance of certain manual tasks. Such cases can be considered an occupational disease. This study was addressed to the question: Why do some people develop carpal tunnel syndrome while others do not? Two matching female populations, one with a known history of carpal tunnel syndrome and one without a known history of carpal tunnel syndrome, were selected and differences in hand size and work methods were studied. Both populations were employed in the same production sewing jobs. While differences in hand size were not found, use of forceful exertions and of deviated wrists and pinch hand position - particularly during forceful exertions - were found to be associated with carpal tunnel syndrome.", "contents": "Carpal tunnel syndrome and selected personal attributes. Carpal tunnel syndrome has been reported to occur between two and ten times as frequently in women as in men. It also has been reported that this syndrome is often associated with the performance of certain manual tasks. Such cases can be considered an occupational disease. This study was addressed to the question: Why do some people develop carpal tunnel syndrome while others do not? Two matching female populations, one with a known history of carpal tunnel syndrome and one without a known history of carpal tunnel syndrome, were selected and differences in hand size and work methods were studied. Both populations were employed in the same production sewing jobs. While differences in hand size were not found, use of forceful exertions and of deviated wrists and pinch hand position - particularly during forceful exertions - were found to be associated with carpal tunnel syndrome."}
{"id": "PMID:469614", "title": "Pulmonary abnormalities in art glassblowers.", "content": "Art glassblowing is a profession bringing the lung in close contact with many potential hazards. Forty-seven art glassblowers with a mean age of 34.5 years answered a questionnaire and had pulmonary function studies performed. While this was generally a young, healthy population, 21% had \"usual cough\" and 31% had wheezing. Pulmonary functions were most often normal. However, using multiple linear regression analysis, the functions associated with volume, VC and FEV1 showed a significant drop with increase in the total lifetime hours exposed to glassblowing. Both the presence of a cough and the production of phlegm were related to hours of exposure. Thus it seems there may be some unknown toxic effect of art glassblowing which in the future may prove to be hazardous to health.", "contents": "Pulmonary abnormalities in art glassblowers. Art glassblowing is a profession bringing the lung in close contact with many potential hazards. Forty-seven art glassblowers with a mean age of 34.5 years answered a questionnaire and had pulmonary function studies performed. While this was generally a young, healthy population, 21% had \"usual cough\" and 31% had wheezing. Pulmonary functions were most often normal. However, using multiple linear regression analysis, the functions associated with volume, VC and FEV1 showed a significant drop with increase in the total lifetime hours exposed to glassblowing. Both the presence of a cough and the production of phlegm were related to hours of exposure. Thus it seems there may be some unknown toxic effect of art glassblowing which in the future may prove to be hazardous to health."}
{"id": "PMID:469615", "title": "Subclinical effects of chronic increased lead absorption--a prospective study. II. Results of baseline neurologic testing.", "content": "This report summarizes the results of baseline neurologic testing in a group of apparently healthy workers from a secondary lead smelter and a group of controls from nearby aluminum processing plants. The test battery included a standard neurologic examination nerve conduction measurements, quantitative oculomotor function tests and detailed audiologic studies. Lead workers and controls were intermixed so that the examiners were unaware of the status of any individual being tested. Although the lead workers reported significantly more neurologic symptoms than the controls, relatively few differences were found on quantitative neurologic testing. Decreased deep tendon reflexes occured more frequently in the lead workers than in the controls (22% vs. 11%) but the difference was of borderline significance (p=0.06) and other signs of peripheral neuropathy occurred with equal frequency in both groups. The mean motor conduction velocity and sensory latency measurements were not significantly different in the lead workers and in the controls and, of the six oculomotor function measurements, only the mean accuracy of saccadic eye movements was significantly (p less than 0.01) different in the two groups. High frequency hearing loss occurred with equal frequency and severity in both groups, consistent with the level of noise exposure in the lead and control plants.", "contents": "Subclinical effects of chronic increased lead absorption--a prospective study. II. Results of baseline neurologic testing. This report summarizes the results of baseline neurologic testing in a group of apparently healthy workers from a secondary lead smelter and a group of controls from nearby aluminum processing plants. The test battery included a standard neurologic examination nerve conduction measurements, quantitative oculomotor function tests and detailed audiologic studies. Lead workers and controls were intermixed so that the examiners were unaware of the status of any individual being tested. Although the lead workers reported significantly more neurologic symptoms than the controls, relatively few differences were found on quantitative neurologic testing. Decreased deep tendon reflexes occured more frequently in the lead workers than in the controls (22% vs. 11%) but the difference was of borderline significance (p=0.06) and other signs of peripheral neuropathy occurred with equal frequency in both groups. The mean motor conduction velocity and sensory latency measurements were not significantly different in the lead workers and in the controls and, of the six oculomotor function measurements, only the mean accuracy of saccadic eye movements was significantly (p less than 0.01) different in the two groups. High frequency hearing loss occurred with equal frequency and severity in both groups, consistent with the level of noise exposure in the lead and control plants."}
{"id": "PMID:469619", "title": "Polyvinyl chloride pneumoconiosis: epidemiological study of exposed workers.", "content": "Among 1216 workers who were employed in a polyvinyl chloride production factory and who had had no previous dust exposure elsewhere, 20 cases of pneumoconiosis were found. Chest x-ray abnormalities were characterized by limited profusion, irregular type and low gravity. All 20 workers had been exposed to high PVC dust levels. The chest x-ray changes were observed after a minimum exposure of five years and, in a small percentage of cases, were associated with slight restrictive respiratory function impairments. Moreover, in the whole group of workers 388 cases (31.9%) were found with non-specific x-ray abnormalities mainly related to age and smoking.", "contents": "Polyvinyl chloride pneumoconiosis: epidemiological study of exposed workers. Among 1216 workers who were employed in a polyvinyl chloride production factory and who had had no previous dust exposure elsewhere, 20 cases of pneumoconiosis were found. Chest x-ray abnormalities were characterized by limited profusion, irregular type and low gravity. All 20 workers had been exposed to high PVC dust levels. The chest x-ray changes were observed after a minimum exposure of five years and, in a small percentage of cases, were associated with slight restrictive respiratory function impairments. Moreover, in the whole group of workers 388 cases (31.9%) were found with non-specific x-ray abnormalities mainly related to age and smoking."}
{"id": "PMID:469620", "title": "Delivery outcome in women employed in medical occupations in Sweden.", "content": "A study was made of the delivery outcome in women working in medical occupations in Sweden. One cohort of approximately 1500 women who worked in 31 hospitals for chronic diseases during the period 1965-1975 and who gave birth during those years was studied. Also all women working in medical occupations and delivered during the years 1973-1975 were investigated by matching existing data registers. It was found that caesarian sections and vacuum extractions were used more frequently than expected. A significantly increased perinatal death rate was noted in 1973, but could not be related to any specific occupational exposure known to represent an increased risk. No general increase in malformation rate was seen, but a local cluster, occuring at certain hospitals in the Gothenburg area in 1973-1974 was identified. No support was obtained for the hypothesis that the use of soap containing hexachlorophene is associated with an increased risk of perinatal death or malformation.", "contents": "Delivery outcome in women employed in medical occupations in Sweden. A study was made of the delivery outcome in women working in medical occupations in Sweden. One cohort of approximately 1500 women who worked in 31 hospitals for chronic diseases during the period 1965-1975 and who gave birth during those years was studied. Also all women working in medical occupations and delivered during the years 1973-1975 were investigated by matching existing data registers. It was found that caesarian sections and vacuum extractions were used more frequently than expected. A significantly increased perinatal death rate was noted in 1973, but could not be related to any specific occupational exposure known to represent an increased risk. No general increase in malformation rate was seen, but a local cluster, occuring at certain hospitals in the Gothenburg area in 1973-1974 was identified. No support was obtained for the hypothesis that the use of soap containing hexachlorophene is associated with an increased risk of perinatal death or malformation."}
{"id": "PMID:469621", "title": "Changes in feelings of job satisfaction among alcoholics induced by their employer to seek treatment.", "content": "Four hundred eighty-eight alcoholics who had been referred by their employers for treatment were included in the study. Data were collected on various aspects of their feelings of job satisfaction when they entered treatment and three, six and 12 months after they had completed the residential phase of treatment and had returned to work. Questions which related to satisfaction with productivity rather than to enjoyment of the work environment and of co-workers had the highest scores (indicating lowest satisfaction) at admission, and showed the greatest change over time. Improvement in job satisfaction over the follow-up year was directly related to positive change in drinking behavior.", "contents": "Changes in feelings of job satisfaction among alcoholics induced by their employer to seek treatment. Four hundred eighty-eight alcoholics who had been referred by their employers for treatment were included in the study. Data were collected on various aspects of their feelings of job satisfaction when they entered treatment and three, six and 12 months after they had completed the residential phase of treatment and had returned to work. Questions which related to satisfaction with productivity rather than to enjoyment of the work environment and of co-workers had the highest scores (indicating lowest satisfaction) at admission, and showed the greatest change over time. Improvement in job satisfaction over the follow-up year was directly related to positive change in drinking behavior."}
{"id": "PMID:469631", "title": "The pathology of cartilage in chondrodysplasias.", "content": "The pathology of four types of chondrodysplasias, viz., type II achondrogenesis, thanatophoric dwarfism, Saldino-Noonan syndrome, and chondrodysplasia punctata were studied. In each of these disorders, cells with features similar to the chief and dark chondrocytes of normal hyaline cartilage were seen to be altered in different ways. There was a total absence of chief cells in type II achondrogenesis. All the chondrocytes present were of one variety at different states of maturation, with the fully matured cell having features of dark chondrocytes. The absence of chief cells was associated with marked diminution of interlacunar matrix and failure of growth plate development. The chief chondrocytes in thanatophoric dwarfism appeared diminished in number. They were probably abnormal functionally as evident by their lack of cytoplasmic vacuolation and the formation of thick, occasionally branched collagen in the matrix. The growth plate was stunted and poorly developed. Striking changes involving the dark cells were noted in Saldino-Noonan syndrome, where unusually elongated dark cells were found in groups within abnormal cystic spaces. The chief cells were large and contained abnormal cytoplasmic filaments. There was no formation of a growth plate. In chondrodysplasia punctata, the chief cells were enlarged and abnormally vacuolated. The matrix showed excessive aggregates of coarse granular material. In addition, there were focal accumulations of highly abnormal chief and dark cells with abnormal matrix which contained increased amount of keratan sulphate and culminated in spotty calcification.", "contents": "The pathology of cartilage in chondrodysplasias. The pathology of four types of chondrodysplasias, viz., type II achondrogenesis, thanatophoric dwarfism, Saldino-Noonan syndrome, and chondrodysplasia punctata were studied. In each of these disorders, cells with features similar to the chief and dark chondrocytes of normal hyaline cartilage were seen to be altered in different ways. There was a total absence of chief cells in type II achondrogenesis. All the chondrocytes present were of one variety at different states of maturation, with the fully matured cell having features of dark chondrocytes. The absence of chief cells was associated with marked diminution of interlacunar matrix and failure of growth plate development. The chief chondrocytes in thanatophoric dwarfism appeared diminished in number. They were probably abnormal functionally as evident by their lack of cytoplasmic vacuolation and the formation of thick, occasionally branched collagen in the matrix. The growth plate was stunted and poorly developed. Striking changes involving the dark cells were noted in Saldino-Noonan syndrome, where unusually elongated dark cells were found in groups within abnormal cystic spaces. The chief cells were large and contained abnormal cytoplasmic filaments. There was no formation of a growth plate. In chondrodysplasia punctata, the chief cells were enlarged and abnormally vacuolated. The matrix showed excessive aggregates of coarse granular material. In addition, there were focal accumulations of highly abnormal chief and dark cells with abnormal matrix which contained increased amount of keratan sulphate and culminated in spotty calcification."}
{"id": "PMID:469633", "title": "Vascular permeability in the rat gingiva. A model of vessel response in chronic inflammation.", "content": "The vascular permeability of the vessels of clinically normal gingiva of rats was studied using the colloidal carbon technique. The connective tissue situated underneath the keratinised epithelium was normal, but that subjacent to the non-keratinised epithelium showed some degree of chronic inflammation and as a rule the vessels of the area exhibited increased vascular permeability. In the buccal gingiva the vessels labelled with carbon form loops situated 200 micrometers below the marginal gingiva, while in the interdental gingiva the altered vessels are just below the superficial epithelium. On electron microscopy the vessels in the inflamed areas showed many open endothelial junctions, and also pseudo-fenestration, and endoplasmic vesicles full of carbon. Little is known about vascular changes in chronic inflammation, and the rat gingiva seems to be a suitable model for their study.", "contents": "Vascular permeability in the rat gingiva. A model of vessel response in chronic inflammation. The vascular permeability of the vessels of clinically normal gingiva of rats was studied using the colloidal carbon technique. The connective tissue situated underneath the keratinised epithelium was normal, but that subjacent to the non-keratinised epithelium showed some degree of chronic inflammation and as a rule the vessels of the area exhibited increased vascular permeability. In the buccal gingiva the vessels labelled with carbon form loops situated 200 micrometers below the marginal gingiva, while in the interdental gingiva the altered vessels are just below the superficial epithelium. On electron microscopy the vessels in the inflamed areas showed many open endothelial junctions, and also pseudo-fenestration, and endoplasmic vesicles full of carbon. Little is known about vascular changes in chronic inflammation, and the rat gingiva seems to be a suitable model for their study."}
{"id": "PMID:469634", "title": "The histogenesis of nerve sheath myxoma: report of a case with electron microscopy.", "content": "The case is described of a 71-yr-old female with a tumour in the pulp of the left index finger. The histology was that of a myxoma of nerve sheath or Pacinian neurofibroma. Electron microscopy of the tumour provides convincing evidence for an origin from the perineural cells. The location of these tumours, suggestive histology and perineural origin provides support for the belief that they arise from sensory nerve endings.", "contents": "The histogenesis of nerve sheath myxoma: report of a case with electron microscopy. The case is described of a 71-yr-old female with a tumour in the pulp of the left index finger. The histology was that of a myxoma of nerve sheath or Pacinian neurofibroma. Electron microscopy of the tumour provides convincing evidence for an origin from the perineural cells. The location of these tumours, suggestive histology and perineural origin provides support for the belief that they arise from sensory nerve endings."}
{"id": "PMID:469635", "title": "Giant pseudopolyposis in granulomatous colitis.", "content": "A case is described of an unusual combination of polypois and inflammatory bowel disease of a granulomatous type. A striking feature is the complexity of the polypoidal lesions. The relationship between these lesions and the granulomatous colitis which is probably a variant of Crohn's disease is discussed.", "contents": "Giant pseudopolyposis in granulomatous colitis. A case is described of an unusual combination of polypois and inflammatory bowel disease of a granulomatous type. A striking feature is the complexity of the polypoidal lesions. The relationship between these lesions and the granulomatous colitis which is probably a variant of Crohn's disease is discussed."}
{"id": "PMID:469636", "title": "Paranodal vascular spread in breast cancer with axillary node involvement.", "content": "The presence of tumour cells in the efferent paranodal vessels of tumour-bearing axillary lymph nodes from patients with operable breast carcinoma was associated with a significant reduction in survival time at 5 yr compared to cases with node involvement in which these vessels did not contain tumour cells.", "contents": "Paranodal vascular spread in breast cancer with axillary node involvement. The presence of tumour cells in the efferent paranodal vessels of tumour-bearing axillary lymph nodes from patients with operable breast carcinoma was associated with a significant reduction in survival time at 5 yr compared to cases with node involvement in which these vessels did not contain tumour cells."}
{"id": "PMID:469638", "title": "Effect of gonadectomy and oestrogen administration on the response of lymph-node post-capillary venules to infection with Toxoplasma gondii.", "content": "The effects of gonadectomy and oestrogen administration on the response of lymph-node post-capillary venules to infection with Toxoplasma gondii have been studied in mice. The endothelial cells of the post-capillary venules were significantly higher in the female than in the male controls. Oophorectomy had no significant effect while orchidectomy increased the height of the cells. However, hexoestrol administration resulted in a rise of the height to maximum levels in the female and in the male gonadectomised animals. The endothelial cells of male and female control and gonadectomised mice were higher after infection with Toxoplasma while in the infected hexoestrol-treated animals, lower cells were seen. The number of lymphocytes passing through the post-capillary venule wall was increased by gonadectomy and decreased by hexoestrol administration in both sexes. The same pattern was seen in the infected groups. The influence of sex hormones on the post-capillary venules and its possible contribution to the differences seen in the immune response between the two sexes is discussed.", "contents": "Effect of gonadectomy and oestrogen administration on the response of lymph-node post-capillary venules to infection with Toxoplasma gondii. The effects of gonadectomy and oestrogen administration on the response of lymph-node post-capillary venules to infection with Toxoplasma gondii have been studied in mice. The endothelial cells of the post-capillary venules were significantly higher in the female than in the male controls. Oophorectomy had no significant effect while orchidectomy increased the height of the cells. However, hexoestrol administration resulted in a rise of the height to maximum levels in the female and in the male gonadectomised animals. The endothelial cells of male and female control and gonadectomised mice were higher after infection with Toxoplasma while in the infected hexoestrol-treated animals, lower cells were seen. The number of lymphocytes passing through the post-capillary venule wall was increased by gonadectomy and decreased by hexoestrol administration in both sexes. The same pattern was seen in the infected groups. The influence of sex hormones on the post-capillary venules and its possible contribution to the differences seen in the immune response between the two sexes is discussed."}
{"id": "PMID:469639", "title": "Biochemical and pharmacological properties of a neurotoxic protein isolated from the blood serum of heavily burned patients.", "content": "Blood serum of heavily burned patients contains neurotoxic substances which are not present in normal control sera. In the present paper, we describe the purification by gel filtration and ultracentrifugation of such a neurotoxic factor. The purified factor appeared to be a high molecular weight (2 to 3.10(6) daltons) lipoprotein. This factor was present in all the sera of patients with more than 35 per cent of the body surface burned. When injected into rabbits the lipoprotein caused a flattening of the EEG tracing, then trembling and convulsions with bursts of spikes on the EEG. The activity of this neurotoxic substance was enhanced when the permeability of the blood-brain barrier was increased by previous intraventricular injection of collagenase. The presence of such a neurotoxic factor in the blood serum of burned patients, together with the increased serum collagenase activity they exhibit may explain the neurotoxic symptoms observed in them.", "contents": "Biochemical and pharmacological properties of a neurotoxic protein isolated from the blood serum of heavily burned patients. Blood serum of heavily burned patients contains neurotoxic substances which are not present in normal control sera. In the present paper, we describe the purification by gel filtration and ultracentrifugation of such a neurotoxic factor. The purified factor appeared to be a high molecular weight (2 to 3.10(6) daltons) lipoprotein. This factor was present in all the sera of patients with more than 35 per cent of the body surface burned. When injected into rabbits the lipoprotein caused a flattening of the EEG tracing, then trembling and convulsions with bursts of spikes on the EEG. The activity of this neurotoxic substance was enhanced when the permeability of the blood-brain barrier was increased by previous intraventricular injection of collagenase. The presence of such a neurotoxic factor in the blood serum of burned patients, together with the increased serum collagenase activity they exhibit may explain the neurotoxic symptoms observed in them."}
{"id": "PMID:469640", "title": "Biochemical and pharmacological properties of a cardiotoxic factor isolated from the blood serum of burned patients.", "content": "Blood serum of severely burned patients contains several substances which are not present in normal sera. One of these substances, a small protein of an approximate molecular weight of 12 to 14,000 daltons displayed a toxic action on the circulatory system. This cardiotoxic factor was obtained in a purified form by alcohol precipitation followed by gel filtration and carboxymethyl cellulose chromatography. The purified preparation seems to be a low molecular weight protein of about 8000 daltons. The biological effects of this substance consist essentially of alterations of the ECG pattern, indicating decreased cardiac output and ischaemia of the cardial muscle. The blood pressure decreases and respiratory function is also altered. The presence of such a toxic factor in sufficient concentration in the blood serum may explain the cardiovascular complications observed in some burned patients.", "contents": "Biochemical and pharmacological properties of a cardiotoxic factor isolated from the blood serum of burned patients. Blood serum of severely burned patients contains several substances which are not present in normal sera. One of these substances, a small protein of an approximate molecular weight of 12 to 14,000 daltons displayed a toxic action on the circulatory system. This cardiotoxic factor was obtained in a purified form by alcohol precipitation followed by gel filtration and carboxymethyl cellulose chromatography. The purified preparation seems to be a low molecular weight protein of about 8000 daltons. The biological effects of this substance consist essentially of alterations of the ECG pattern, indicating decreased cardiac output and ischaemia of the cardial muscle. The blood pressure decreases and respiratory function is also altered. The presence of such a toxic factor in sufficient concentration in the blood serum may explain the cardiovascular complications observed in some burned patients."}
{"id": "PMID:469641", "title": "The mechanical, enzymatic and morphological changes in acute pulmonary insufficiency following the production of a haematoma in rabbits.", "content": "The production of a haematoma in the hindleg of rabbits represents a simplification of post-traumatic models. The lungs, one of the target organs, show similar micromorphological alterations to those described in traumatised human beings. The lungs of rabbits, bearing a hindleg haematoma for 24 hr, showed a significantly increased incidence of micromorphological lung alterations compared to the control group. Moreover, measurement revealed a significantly decreased lung compliance within the haematoma groups. It was demonstrated that whether the haematoma was subcutaneous or intramuscular, similar alterations were seen in lung histology, and in lung compliance values. Studies on the activity of superoxide dismutase in lung tissue homogenates demonstrated a significant decrease in those rabbits having had a haematoma intramuscularly for 24 hr.", "contents": "The mechanical, enzymatic and morphological changes in acute pulmonary insufficiency following the production of a haematoma in rabbits. The production of a haematoma in the hindleg of rabbits represents a simplification of post-traumatic models. The lungs, one of the target organs, show similar micromorphological alterations to those described in traumatised human beings. The lungs of rabbits, bearing a hindleg haematoma for 24 hr, showed a significantly increased incidence of micromorphological lung alterations compared to the control group. Moreover, measurement revealed a significantly decreased lung compliance within the haematoma groups. It was demonstrated that whether the haematoma was subcutaneous or intramuscular, similar alterations were seen in lung histology, and in lung compliance values. Studies on the activity of superoxide dismutase in lung tissue homogenates demonstrated a significant decrease in those rabbits having had a haematoma intramuscularly for 24 hr."}
{"id": "PMID:469642", "title": "Patella displacement and osteoarthrosis of the knee joint in mice.", "content": "Surgical stabilisation of the normally medially--subluxing patella of STR/ORT male mice, prevented the normal development of osteoarthrosis in the medial compartment of the femoro-tibial joint. Surgically produced medial dislocation of the patella in non-arthrosis-prone male CBA/ORT mice often gave rise to osteoarthrosis of the medial condyles. This, with further evidence from previous studies, strongly indicates that the high incidence of degenerative joint disease in male STR/ORT mice occurs because of a tendency of the patella to dislocate medially due to some unknown cause.", "contents": "Patella displacement and osteoarthrosis of the knee joint in mice. Surgical stabilisation of the normally medially--subluxing patella of STR/ORT male mice, prevented the normal development of osteoarthrosis in the medial compartment of the femoro-tibial joint. Surgically produced medial dislocation of the patella in non-arthrosis-prone male CBA/ORT mice often gave rise to osteoarthrosis of the medial condyles. This, with further evidence from previous studies, strongly indicates that the high incidence of degenerative joint disease in male STR/ORT mice occurs because of a tendency of the patella to dislocate medially due to some unknown cause."}
{"id": "PMID:469643", "title": "In-vitro modification of membrane receptors on cells of the mononuclear phagocyte system.", "content": "EA and EAC receptors have been studied on non-elicited and paraffin-induced macrophages, under a variety of culture conditions in vitro, for up to 7 days. A large decrease in the number of macrophages showing EAC receptors was found after treatment of the cells with BCG, but not \"inert\" particles such as latex and zymosan. This was reversible by 7 days. In the presence of a toxic material, Al(OH)3, both EA and EAC receptors were partially lost. The results obtained have been related to previous results obtained with cryostat sections of human leprosy skin lesions.", "contents": "In-vitro modification of membrane receptors on cells of the mononuclear phagocyte system. EA and EAC receptors have been studied on non-elicited and paraffin-induced macrophages, under a variety of culture conditions in vitro, for up to 7 days. A large decrease in the number of macrophages showing EAC receptors was found after treatment of the cells with BCG, but not \"inert\" particles such as latex and zymosan. This was reversible by 7 days. In the presence of a toxic material, Al(OH)3, both EA and EAC receptors were partially lost. The results obtained have been related to previous results obtained with cryostat sections of human leprosy skin lesions."}
{"id": "PMID:469644", "title": "Quantitative comparisons of the pulmonary toxicity of tobacco and NSM smoke.", "content": "Smoking elicits a panacinar rise in pulmonary alveolar lysosomal activity that is thought to correlate with the irritancy of the smoke. Quantitative image analysis of lung sections stained histochemically for acid phosphatase indicates that tobacco smoke is significantly more irritant than NSM smoke.", "contents": "Quantitative comparisons of the pulmonary toxicity of tobacco and NSM smoke. Smoking elicits a panacinar rise in pulmonary alveolar lysosomal activity that is thought to correlate with the irritancy of the smoke. Quantitative image analysis of lung sections stained histochemically for acid phosphatase indicates that tobacco smoke is significantly more irritant than NSM smoke."}
{"id": "PMID:469645", "title": "Aggressive osteoblastoma.", "content": "The clinical, radiological and pathological features of two cases of an osteogenic tumour with long survival are described. The tumours have the histological pattern of benign osteoblastoma with other more cellular and aggressive features suggestive of a low grade osteosarcoma. They are locally invasive but the absence of metastases indicates that separation from both entities is justified clinically and pathologically. The term aggressive osteoblastoma is suggested.", "contents": "Aggressive osteoblastoma. The clinical, radiological and pathological features of two cases of an osteogenic tumour with long survival are described. The tumours have the histological pattern of benign osteoblastoma with other more cellular and aggressive features suggestive of a low grade osteosarcoma. They are locally invasive but the absence of metastases indicates that separation from both entities is justified clinically and pathologically. The term aggressive osteoblastoma is suggested."}
{"id": "PMID:469646", "title": "Granulomatous inflammation in normal and athymic mice infected with schistosoma mansoni: an ultrastructural study.", "content": "Inflammatory cell types and their interrelationships were studied in hepatic schistosome egg granulomas by correlated light and electron microscopy in thymus-intact and athymic mice. Intact animals developed large granulomas composed of phagocytes, stimulated macrophages, epithelioid cells, eosinophils and mast cells. The lesions peaked in size between 10--14 weeks after infection and tended to heal after 16 weeks. In athymic mice only phagocytes, stimulated macrophages and possible epithelioid cells appeared in the granulomas which were much smaller and less well organised than in intact mice. Virtually no eosinophils or mast cells entered the granulomas. These findings support the idea that development of granulomatous inflammation per se is not determined by CMI, but T cell co-operation seems to be required for its full expression in schistosomiasis. Since CMI closely regulates ingress of eosinophils and mast cells into the granuloma, the nature of T cell co-operation probably occurs at several levels in this complex infectious granuloma.", "contents": "Granulomatous inflammation in normal and athymic mice infected with schistosoma mansoni: an ultrastructural study. Inflammatory cell types and their interrelationships were studied in hepatic schistosome egg granulomas by correlated light and electron microscopy in thymus-intact and athymic mice. Intact animals developed large granulomas composed of phagocytes, stimulated macrophages, epithelioid cells, eosinophils and mast cells. The lesions peaked in size between 10--14 weeks after infection and tended to heal after 16 weeks. In athymic mice only phagocytes, stimulated macrophages and possible epithelioid cells appeared in the granulomas which were much smaller and less well organised than in intact mice. Virtually no eosinophils or mast cells entered the granulomas. These findings support the idea that development of granulomatous inflammation per se is not determined by CMI, but T cell co-operation seems to be required for its full expression in schistosomiasis. Since CMI closely regulates ingress of eosinophils and mast cells into the granuloma, the nature of T cell co-operation probably occurs at several levels in this complex infectious granuloma."}
{"id": "PMID:469647", "title": "Ultrastructural types of cell in adrenal cortical adenoma with primary aldosteronism.", "content": "By means of electron microscopy, three types of cell were identified in the adrenal cortical adenomas obtained by surgery from three patients with primary aldosteronism. They were zona glomerulosa-type cells, compact cells and clear cells. The former two types of cell were quite similar to those found in the zona glomerulosa and the zona reticularis respectively. The clear cell with large lipid vacuoles possessed mitochondria similar to those found in the outer zona fasciculata. The ultrastructure of the vacuoles was rather complex and their relationship to those in the zona fasciculata cell was not clear. Glycogen particles in the beta-form, which were present in the zona glomerulosa and reticularis cell but not in the zona fasciculata cell, were observed within the cytoplasm of this tumour cell. The clear cells of the tumours were therefore considered to possess features of both the zona glomerulosa and the zona fasciculata cell.", "contents": "Ultrastructural types of cell in adrenal cortical adenoma with primary aldosteronism. By means of electron microscopy, three types of cell were identified in the adrenal cortical adenomas obtained by surgery from three patients with primary aldosteronism. They were zona glomerulosa-type cells, compact cells and clear cells. The former two types of cell were quite similar to those found in the zona glomerulosa and the zona reticularis respectively. The clear cell with large lipid vacuoles possessed mitochondria similar to those found in the outer zona fasciculata. The ultrastructure of the vacuoles was rather complex and their relationship to those in the zona fasciculata cell was not clear. Glycogen particles in the beta-form, which were present in the zona glomerulosa and reticularis cell but not in the zona fasciculata cell, were observed within the cytoplasm of this tumour cell. The clear cells of the tumours were therefore considered to possess features of both the zona glomerulosa and the zona fasciculata cell."}
{"id": "PMID:469648", "title": "Migration of peritoneal polymorphonuclear leucocytes in the rat.", "content": "The migration of PMNs into inflammatory sites in the rat has been studied using elicited peritoneal cells. These cells exhibit normal random migration and phagocytosis in vitro but their chemotactic response towards activated serum is significantly reduced when compared to cells isolated from peripheral blood. When injected intravenously, peritoneal cells not only show a greatly diminished ability to accumulate in inflammatory exudates but also block the migration of peripheral PMNs. Thus while PMNs obtained from the peritoneum may be suitable for some studies, they are not representative of peripheral cells with respect to their ability to accumulate in inflammatory exudates due to implanted inert sponges.", "contents": "Migration of peritoneal polymorphonuclear leucocytes in the rat. The migration of PMNs into inflammatory sites in the rat has been studied using elicited peritoneal cells. These cells exhibit normal random migration and phagocytosis in vitro but their chemotactic response towards activated serum is significantly reduced when compared to cells isolated from peripheral blood. When injected intravenously, peritoneal cells not only show a greatly diminished ability to accumulate in inflammatory exudates but also block the migration of peripheral PMNs. Thus while PMNs obtained from the peritoneum may be suitable for some studies, they are not representative of peripheral cells with respect to their ability to accumulate in inflammatory exudates due to implanted inert sponges."}
{"id": "PMID:469649", "title": "Early bronchiolar damage following paraquat poisoning in mice.", "content": "Paraquat causes focal intracellular oedema of the terminal bronchiolar epithelial cells and focal subpleural atelectasis with thickening of the interalveolar septa 1 hr after the administration of an LD50. These changes are progressive, and lead to panacinar atelectasis with necrosis plus sloughing of epithelial cells in many terminal bronchioles. Radioactive phosphatidyl choline (PC) is recoverable by lavage within 90 s of the administration of tritiated palmitate, which supports previous suggestions that one source of pulmonary surfactant is rapid secretion by the terminal bronchiole. Paraquat causes a reduction in the relative amounts of radioactive PC that are recoverable from the airways within 90 s of giving tritiated palmitate. A deficiency of pulmonary surfactant of bronchiolar origin is implicated, at least in part, in the pathogenesis of the acute phase of the paraquat lesion in mice.", "contents": "Early bronchiolar damage following paraquat poisoning in mice. Paraquat causes focal intracellular oedema of the terminal bronchiolar epithelial cells and focal subpleural atelectasis with thickening of the interalveolar septa 1 hr after the administration of an LD50. These changes are progressive, and lead to panacinar atelectasis with necrosis plus sloughing of epithelial cells in many terminal bronchioles. Radioactive phosphatidyl choline (PC) is recoverable by lavage within 90 s of the administration of tritiated palmitate, which supports previous suggestions that one source of pulmonary surfactant is rapid secretion by the terminal bronchiole. Paraquat causes a reduction in the relative amounts of radioactive PC that are recoverable from the airways within 90 s of giving tritiated palmitate. A deficiency of pulmonary surfactant of bronchiolar origin is implicated, at least in part, in the pathogenesis of the acute phase of the paraquat lesion in mice."}
{"id": "PMID:469650", "title": "Amyloid in localised deposits and plasmacytomas of the respiratory tract.", "content": "Clinical and histological features of 25 cases with amyloid deposits in the respiratory tract are described. The false cord is the commonest single site of such \"primary\" amyloid involvement. Evidence of extensive involvement and replacement of seromucinous glands by amyloid was seen in each case. Fourteen cases of plasmacytoma with \"secondary\" amyloid deposits are also described. The nose and nasopharynx were principally involved as with respiratory tract plasmacytomas in general but a similar association of the amyloid with seromucinous glands was also seen. Electron microscopy showed early involvement of the basal lamina of the duct of a seromucinous gland by amyloid in one case. Possible reasons for the association of amyloid precursors in respiratory tract amyloid with seromucinous glands are given.", "contents": "Amyloid in localised deposits and plasmacytomas of the respiratory tract. Clinical and histological features of 25 cases with amyloid deposits in the respiratory tract are described. The false cord is the commonest single site of such \"primary\" amyloid involvement. Evidence of extensive involvement and replacement of seromucinous glands by amyloid was seen in each case. Fourteen cases of plasmacytoma with \"secondary\" amyloid deposits are also described. The nose and nasopharynx were principally involved as with respiratory tract plasmacytomas in general but a similar association of the amyloid with seromucinous glands was also seen. Electron microscopy showed early involvement of the basal lamina of the duct of a seromucinous gland by amyloid in one case. Possible reasons for the association of amyloid precursors in respiratory tract amyloid with seromucinous glands are given."}
{"id": "PMID:469651", "title": "The pathology of cot deaths.", "content": "An examination of the pathological features of 12 consecutive cases of sudden death in infancy was undertaken with particular reference to the lung changes used a combined radiological and pathological study of formalin vapour-inflated lungs. Certain common features in the cases in which there are no post mortem changes to explain death (typical cot deaths) are described and compared with cases with an acceptable cause of death and with control cases. It was concluded that severe dehydration is a potentially avoidable factor underlying cot deaths and many other sudden infant deaths with no explainable causes; that petechial haemorrhages are a constant feature of cot deaths and indicate a respiratory/hypoxic mode of death; that absence of petechiae indicates some other explanation for death. It is suggested that the minor respiratory or other infections which are usually present at autopsy in cot deaths may sometimes exaggerate a preexisting state of dehydration and that the dehydration may contribute more directly to death than the infection.", "contents": "The pathology of cot deaths. An examination of the pathological features of 12 consecutive cases of sudden death in infancy was undertaken with particular reference to the lung changes used a combined radiological and pathological study of formalin vapour-inflated lungs. Certain common features in the cases in which there are no post mortem changes to explain death (typical cot deaths) are described and compared with cases with an acceptable cause of death and with control cases. It was concluded that severe dehydration is a potentially avoidable factor underlying cot deaths and many other sudden infant deaths with no explainable causes; that petechial haemorrhages are a constant feature of cot deaths and indicate a respiratory/hypoxic mode of death; that absence of petechiae indicates some other explanation for death. It is suggested that the minor respiratory or other infections which are usually present at autopsy in cot deaths may sometimes exaggerate a preexisting state of dehydration and that the dehydration may contribute more directly to death than the infection."}
{"id": "PMID:469652", "title": "Carcinoma of the neck in young children: a light and electron microscopic study.", "content": "Two neck tumours in young children with similar clinical features were compared using light and electron microscopy. Both tumours consisted of uniform sheets of epithelial-like cells and there were scattered stromal elements in one. Tonofilaments in sheets or bundles reminiscent of squamous epithelium were striking in the epithelial cells. Intercellular junctions were frequent but true desmosomes were not seen. Most cells contained a few secretory droplets. Occasional secretory cells, foam cells and cilia were observed. Since these tumours were not sited superficially they are unlikely to have been derived from skin epithelium. An origin in branchial sinus remnants capable of differentiation towards squamous and ciliated epithelia is suggested.", "contents": "Carcinoma of the neck in young children: a light and electron microscopic study. Two neck tumours in young children with similar clinical features were compared using light and electron microscopy. Both tumours consisted of uniform sheets of epithelial-like cells and there were scattered stromal elements in one. Tonofilaments in sheets or bundles reminiscent of squamous epithelium were striking in the epithelial cells. Intercellular junctions were frequent but true desmosomes were not seen. Most cells contained a few secretory droplets. Occasional secretory cells, foam cells and cilia were observed. Since these tumours were not sited superficially they are unlikely to have been derived from skin epithelium. An origin in branchial sinus remnants capable of differentiation towards squamous and ciliated epithelia is suggested."}
{"id": "PMID:469653", "title": "The inflammatory response to endotoxin.", "content": "A typical inflammatory response resulted from the intravenous injection of endotoxin (E. coli) into living rabbits. Each rabbit was studied at three levels: the microvasculature and supporting tissue in the ear chamber was observed microscopically (up to X200) before, during, and at regular intervals following the injection of endotoxin; leucocyte and platelet counts were made periodically throughout each experiment; and tissue samples for histological study were obtained from each rabbit prior to death. The animal was anaesthetised before histological samples were secured. Within minutes after the intravenous injection of endotoxin, leucocytes were observed sticking to the endothelial cells lining the venules and the arterioles. Emboli appeared in the microcirculation within 10 min. Swelling of the microvascular endothelial cells was evident at 1 hr; oedema and extravasation of the cellular elements followed. The rectal temperature and leucocyte and platelet counts all fell within 10 min. of endotoxin. Histological examination of tissue from the ear chamber and visceral organs showed inflammatory changes. Congestion of the microvasculature, swelling of the endothelial cells, and margination and migration of neutrophils were common histological features in all organs. The earliest cells affected appeared to be the leukocyte and platelet.", "contents": "The inflammatory response to endotoxin. A typical inflammatory response resulted from the intravenous injection of endotoxin (E. coli) into living rabbits. Each rabbit was studied at three levels: the microvasculature and supporting tissue in the ear chamber was observed microscopically (up to X200) before, during, and at regular intervals following the injection of endotoxin; leucocyte and platelet counts were made periodically throughout each experiment; and tissue samples for histological study were obtained from each rabbit prior to death. The animal was anaesthetised before histological samples were secured. Within minutes after the intravenous injection of endotoxin, leucocytes were observed sticking to the endothelial cells lining the venules and the arterioles. Emboli appeared in the microcirculation within 10 min. Swelling of the microvascular endothelial cells was evident at 1 hr; oedema and extravasation of the cellular elements followed. The rectal temperature and leucocyte and platelet counts all fell within 10 min. of endotoxin. Histological examination of tissue from the ear chamber and visceral organs showed inflammatory changes. Congestion of the microvasculature, swelling of the endothelial cells, and margination and migration of neutrophils were common histological features in all organs. The earliest cells affected appeared to be the leukocyte and platelet."}
{"id": "PMID:469654", "title": "Ultrastructural observations on the capillaries of human thyroid tumours.", "content": "Ultrastructure of the capillaries of malignant and benign thyroid tumours has been examined. The material consisted of biopsies from six cases of thyroid papillary carcinoma, one case of follicular (foetal type) adenoma and six cases of nodular adenomatous goitre. In the group of nodular adenomatous goitre and in the follicular adenoma, the capillary wall was made up of fenestrated endothelium similar to that of capillaries of normal human thyroid. The fenestrae occupied a large area of the endothelial wall. Micro- and macropinocytotic vesicles were frequent in the endothelial cytoplasm. In the thyroid carcinomas the papillary structures always contained numerous capillaries with fenestrated endothelium. The microfollicular area and the solid tumoral areas of the papillary carcinoma showed occasional capillaries with fenestrated endothelium, but many capillaries were lined with continuous endothelium. The capillaries in all the specimens were surrounded externally by a continuous basement membrane which was frequently bilaminate or multilaminate. This study indicates that capillaries with fenestrated endothelium are characteristic of thyroid tumours which arise from follicular cells.", "contents": "Ultrastructural observations on the capillaries of human thyroid tumours. Ultrastructure of the capillaries of malignant and benign thyroid tumours has been examined. The material consisted of biopsies from six cases of thyroid papillary carcinoma, one case of follicular (foetal type) adenoma and six cases of nodular adenomatous goitre. In the group of nodular adenomatous goitre and in the follicular adenoma, the capillary wall was made up of fenestrated endothelium similar to that of capillaries of normal human thyroid. The fenestrae occupied a large area of the endothelial wall. Micro- and macropinocytotic vesicles were frequent in the endothelial cytoplasm. In the thyroid carcinomas the papillary structures always contained numerous capillaries with fenestrated endothelium. The microfollicular area and the solid tumoral areas of the papillary carcinoma showed occasional capillaries with fenestrated endothelium, but many capillaries were lined with continuous endothelium. The capillaries in all the specimens were surrounded externally by a continuous basement membrane which was frequently bilaminate or multilaminate. This study indicates that capillaries with fenestrated endothelium are characteristic of thyroid tumours which arise from follicular cells."}
{"id": "PMID:469655", "title": "Spindle cell sarcoma of the pulmonary trunk: a case report with histochemistry and electron microscopy.", "content": "A further case of a spindle cell sarcoma of the pulmonary trunk is reported, with extension to the pulmonary valve and lungs. Some spindle cells contained glycogen and their cell walls acid mucosubstances. Electron microscopy showed two types of cell. The more common were polyhedral with poorly arranged fibrils in the cytoplasm and an abundant fuzzy coat. About 5 per cent of the cells contained neurosecretory granules.", "contents": "Spindle cell sarcoma of the pulmonary trunk: a case report with histochemistry and electron microscopy. A further case of a spindle cell sarcoma of the pulmonary trunk is reported, with extension to the pulmonary valve and lungs. Some spindle cells contained glycogen and their cell walls acid mucosubstances. Electron microscopy showed two types of cell. The more common were polyhedral with poorly arranged fibrils in the cytoplasm and an abundant fuzzy coat. About 5 per cent of the cells contained neurosecretory granules."}
{"id": "PMID:469656", "title": "Central nervous system alterations as sequelae of Venezuelan equine encephalitis virus infection in the rat.", "content": "Alterations of the Central Nervous System (CNS) in rats surviving acute infection with a virulent strain of Venezuelan Equine Encephalitis (VEE) virus were studied by light and electron microscopy. Cavitary necrosis of the cerebral cortex, macrophage activity and degenerative axonal changes were considered to be sequelae of the lesions induced during the acute phase of the infection. Mononuclear cell infiltrates of the neuropil, 3 mth after inoculation, were related to the immune response of the host. Focal lesions and mononuclear cell activity in the brain are thought to be the equivalent of the lesions induced in the CNS of humans during VEE virus infection. The findings are discussed in the light of recent reports of cerebral dysfunction occurring as a sequel of VEE virus infection in children.", "contents": "Central nervous system alterations as sequelae of Venezuelan equine encephalitis virus infection in the rat. Alterations of the Central Nervous System (CNS) in rats surviving acute infection with a virulent strain of Venezuelan Equine Encephalitis (VEE) virus were studied by light and electron microscopy. Cavitary necrosis of the cerebral cortex, macrophage activity and degenerative axonal changes were considered to be sequelae of the lesions induced during the acute phase of the infection. Mononuclear cell infiltrates of the neuropil, 3 mth after inoculation, were related to the immune response of the host. Focal lesions and mononuclear cell activity in the brain are thought to be the equivalent of the lesions induced in the CNS of humans during VEE virus infection. The findings are discussed in the light of recent reports of cerebral dysfunction occurring as a sequel of VEE virus infection in children."}
{"id": "PMID:469657", "title": "The role of resident and exudate macrophages in multinucleate giant cell formation.", "content": "Peroxidase cytochemistry which differentiates \"resident\" from \"exudate\" peritoneal macrophages in guinea pigs, was used in the investigation of the multinucleate giant cells in foreign body granulomas in the peritoneal cavity of guinea pigs. Only a few, small syncytia (two to three nuclei) displayed the cytochemical characteristics of \"resident\" macrophages. On the other hand, most of the polykarya at the site of inflammation displayed a distribution of peroxidase activity similar to that of \"exudate\" macrophages. Irrespective of whether peroxidase cytochemistry distinguishes between a distinct type of macrophage or a specific functional stage, the evidence indicates that fusion is much more frequent between macrophages with \"exudate\" characteristics. Thus, fusion of these latter cells is responsible for the vast majority of multinucleate giant cells in inflammatory sites. In addition, some form of recognition mechanism between macrophages of similar cytochemical characteristics probably also operates since syncytia exhibited characteristics of either \"exudate\" or \"resident\" macrophages, but never both.", "contents": "The role of resident and exudate macrophages in multinucleate giant cell formation. Peroxidase cytochemistry which differentiates \"resident\" from \"exudate\" peritoneal macrophages in guinea pigs, was used in the investigation of the multinucleate giant cells in foreign body granulomas in the peritoneal cavity of guinea pigs. Only a few, small syncytia (two to three nuclei) displayed the cytochemical characteristics of \"resident\" macrophages. On the other hand, most of the polykarya at the site of inflammation displayed a distribution of peroxidase activity similar to that of \"exudate\" macrophages. Irrespective of whether peroxidase cytochemistry distinguishes between a distinct type of macrophage or a specific functional stage, the evidence indicates that fusion is much more frequent between macrophages with \"exudate\" characteristics. Thus, fusion of these latter cells is responsible for the vast majority of multinucleate giant cells in inflammatory sites. In addition, some form of recognition mechanism between macrophages of similar cytochemical characteristics probably also operates since syncytia exhibited characteristics of either \"exudate\" or \"resident\" macrophages, but never both."}
{"id": "PMID:469658", "title": "Aortic body tumours and hyperplasia in the rat.", "content": "The histologic features of aortic body neoplasia, hyperplasia, and normal aortico-pulmonary paraganglia were described for a series of 56 rats of several strains. Argyrophilic cytoplasmic granules were demonstrated in chief cells of the aortic body lesions, and electron microscopic examination disclosed the presence of electron-dense, membrane-bound granules in these cells. In a series of ageing rats, hyperplasia and neoplasia of the aortico-pulmonary paraganglia occurred more frequently in female WAG/Rij rats than in males of that strain, and more frequently than in males and females of the BN/Bi strain or of the (WAG X BN)F1 hybrid. No apparent causal relationship to chronic hypoxia could be shown, in that no correlation between the development of aortic body neoplasia or hyperplasia and cardiopulmonary disease was found. Aortic body lesions did not appear to occur as part of a multiple endocrinopathy syndrome, although hyperplasia and neoplasia of various endocrine organs occurred relatively frequently in the WAG/Rij strain.", "contents": "Aortic body tumours and hyperplasia in the rat. The histologic features of aortic body neoplasia, hyperplasia, and normal aortico-pulmonary paraganglia were described for a series of 56 rats of several strains. Argyrophilic cytoplasmic granules were demonstrated in chief cells of the aortic body lesions, and electron microscopic examination disclosed the presence of electron-dense, membrane-bound granules in these cells. In a series of ageing rats, hyperplasia and neoplasia of the aortico-pulmonary paraganglia occurred more frequently in female WAG/Rij rats than in males of that strain, and more frequently than in males and females of the BN/Bi strain or of the (WAG X BN)F1 hybrid. No apparent causal relationship to chronic hypoxia could be shown, in that no correlation between the development of aortic body neoplasia or hyperplasia and cardiopulmonary disease was found. Aortic body lesions did not appear to occur as part of a multiple endocrinopathy syndrome, although hyperplasia and neoplasia of various endocrine organs occurred relatively frequently in the WAG/Rij strain."}
{"id": "PMID:469659", "title": "Management of atypical mycobacterial lymphadenitis in childhood: a review based on 380 cases.", "content": "The medical and surgical therapy of 82 cases of atypical mycobacterial adenitis from Dallas and 298 cases from the literature was reviewed. The 92% cure rate in 149 patients with total surgical excision alone was comparable to the 95% cure rate in 156 patients when excision was followed by antituberculous drug therapy. With incision and drainage in 63 patients the cure rate was 16% whether drugs were given or not. Ten patients were initially treated with antituberculous drugs alone and only one was cured. It is concluded that total surgical excision is definitive therapy for this disease and that antituberculous drugs should be used only when surgery cannot be performed or when complete excision is not possible.", "contents": "Management of atypical mycobacterial lymphadenitis in childhood: a review based on 380 cases. The medical and surgical therapy of 82 cases of atypical mycobacterial adenitis from Dallas and 298 cases from the literature was reviewed. The 92% cure rate in 149 patients with total surgical excision alone was comparable to the 95% cure rate in 156 patients when excision was followed by antituberculous drug therapy. With incision and drainage in 63 patients the cure rate was 16% whether drugs were given or not. Ten patients were initially treated with antituberculous drugs alone and only one was cured. It is concluded that total surgical excision is definitive therapy for this disease and that antituberculous drugs should be used only when surgery cannot be performed or when complete excision is not possible."}
{"id": "PMID:469660", "title": "Noninvasive evaluation of ventricular function in cystic fibrosis.", "content": "The cardiac function of 21 patients with cystic fibrosis was studied using radionuclides and M-mode echocardiography. The patients (mean age 13.2 years, range 4 to 27) had a wide range of clinical and pulmonary function abnormalities (mean Shwachman-Kulczycki score 62.1). Decreased right ventricular ejection fraction was found in 13 of 18 patients (72%); an additional four patients had abnormal septal motion on ECHO. Left ventricular ejection fraction was abnormal at rest in four patients (19%); an additional four patients had borderline low LVEF. The LV pre-ejection period to ejection time ratio increased significantly (i.e., worsening LV performance) with deterioration of S-K score, chest radiograph score, and forced expiratory volume in 1 second. Three of eight patients with normal LVEF at rest had an abnormal response to supine bicycle exercise: LVEF fell in two patients and was unchanged in one. Thus evidence of LV dysfunction was observed in seven of 21 (33%) of the patients; four at rest and in three only during exercise.", "contents": "Noninvasive evaluation of ventricular function in cystic fibrosis. The cardiac function of 21 patients with cystic fibrosis was studied using radionuclides and M-mode echocardiography. The patients (mean age 13.2 years, range 4 to 27) had a wide range of clinical and pulmonary function abnormalities (mean Shwachman-Kulczycki score 62.1). Decreased right ventricular ejection fraction was found in 13 of 18 patients (72%); an additional four patients had abnormal septal motion on ECHO. Left ventricular ejection fraction was abnormal at rest in four patients (19%); an additional four patients had borderline low LVEF. The LV pre-ejection period to ejection time ratio increased significantly (i.e., worsening LV performance) with deterioration of S-K score, chest radiograph score, and forced expiratory volume in 1 second. Three of eight patients with normal LVEF at rest had an abnormal response to supine bicycle exercise: LVEF fell in two patients and was unchanged in one. Thus evidence of LV dysfunction was observed in seven of 21 (33%) of the patients; four at rest and in three only during exercise."}
{"id": "PMID:469661", "title": "Norepinephrine metabolism and clinical response to dextroamphetamine in hyperactive boys.", "content": "The 24-hour urinary catecholamine metabolites 3-methoxy-4-hydroxyphenylglycol, normetanephrine, and metanephrine were measured in 23 hyperactive boys and 13 matched healthy controls. The hyperactive children excreted lower MHPG and higher NM (low MHPG/NM ratio) amounts than in controls. The administration of d-amphetamine in the dose of 0.5 mg/kg body weight divided over two doses daily for two weeks decreased MHPG excretion in the hyperactive children. When the hyperactive children group was divided into drug responders and nonresponders according to their pre- and post-treatment scores on the Conners Teacher Questionnaire, d-amphetamine administration decreased MHPG excretion in the responders and did not change it in the nonresponders. Percent decrease in MHPG excretion correlated significantly with percent change in the hyperactivity factor of the questionnaire on the Spearman Rank Order Correlation Coefficient. Pretreatment urinary metabolites did not differentiate the responders from nonresponders. It is suggested that a relationship between CNS norepinephrine metabolism and hyperactivity exists and that d-amphetamine may achieve its therapeutic action in hyperactive children by altering CNS NE metabolism.", "contents": "Norepinephrine metabolism and clinical response to dextroamphetamine in hyperactive boys. The 24-hour urinary catecholamine metabolites 3-methoxy-4-hydroxyphenylglycol, normetanephrine, and metanephrine were measured in 23 hyperactive boys and 13 matched healthy controls. The hyperactive children excreted lower MHPG and higher NM (low MHPG/NM ratio) amounts than in controls. The administration of d-amphetamine in the dose of 0.5 mg/kg body weight divided over two doses daily for two weeks decreased MHPG excretion in the hyperactive children. When the hyperactive children group was divided into drug responders and nonresponders according to their pre- and post-treatment scores on the Conners Teacher Questionnaire, d-amphetamine administration decreased MHPG excretion in the responders and did not change it in the nonresponders. Percent decrease in MHPG excretion correlated significantly with percent change in the hyperactivity factor of the questionnaire on the Spearman Rank Order Correlation Coefficient. Pretreatment urinary metabolites did not differentiate the responders from nonresponders. It is suggested that a relationship between CNS norepinephrine metabolism and hyperactivity exists and that d-amphetamine may achieve its therapeutic action in hyperactive children by altering CNS NE metabolism."}
{"id": "PMID:469662", "title": "Choanal atresia and associated multiple anomalies.", "content": "Seventeen unrelated patients with multiple anomalies of unknown etiology were identified by the presence of choanal atresia. A regularly recurring pattern of associated features involving mental retardation, postnatal growth deficiency, hypogenitalism (males), small ears, cardiac defects, micrognathia, postnatal microcephaly, and ocular coloboma was identified. Choanal atresia when accompanied by multiple anomalies of unknown etiology has a serious prognosis with a predictable pattern of associated defects.", "contents": "Choanal atresia and associated multiple anomalies. Seventeen unrelated patients with multiple anomalies of unknown etiology were identified by the presence of choanal atresia. A regularly recurring pattern of associated features involving mental retardation, postnatal growth deficiency, hypogenitalism (males), small ears, cardiac defects, micrognathia, postnatal microcephaly, and ocular coloboma was identified. Choanal atresia when accompanied by multiple anomalies of unknown etiology has a serious prognosis with a predictable pattern of associated defects."}
{"id": "PMID:469663", "title": "The MURCS association: M\u00fcllerian duct aplasia, renal aplasia, and cervicothoracic somite dysplasia.", "content": "Two patients and 28 others in the literature were ascertained because of congenital vaginal agenesis associated with clinical and/or radiographic evidence of malformations derived from the cervicothoracic somites. In these patients, there was a high incidence of M\u00fcllerian duct aplasia/hypoplasia (96%), renal agenesis and/or ectopy (80%), and abnormalities related to cervicothoracic somite dysplasia, particularly 2 to 4 anomalous vertebrae located between C5-T1 (80%). These consistent findings suggest a distinctive non-random association of malformations: M\u00fcllerian duct (MU) aplasia, renal (R) aplasia, and cervicothoracic somite (CS) dysplasia (MURCS). Identification of one component of the MURCS association suggests the presence of the other associated anomalies. A hypothesis for the embryogenic pathogenesis of the MURCS association is proposed which attributes the malformations to an alteration of the blastemas of the lower cervical-upper thoracic somites, arm buds, and pronephric ducts, all of which have an intimate spatial relationship at the end of the fourth week of fetal life. A presently unidentified teratogen may be one of the possible causes of the MURCS association on the basis of a lack of familial transmission, normal chromosomal studies, and the similar effects of a known teratogen (thalidomide) on the developing genitourinary tract.", "contents": "The MURCS association: M\u00fcllerian duct aplasia, renal aplasia, and cervicothoracic somite dysplasia. Two patients and 28 others in the literature were ascertained because of congenital vaginal agenesis associated with clinical and/or radiographic evidence of malformations derived from the cervicothoracic somites. In these patients, there was a high incidence of M\u00fcllerian duct aplasia/hypoplasia (96%), renal agenesis and/or ectopy (80%), and abnormalities related to cervicothoracic somite dysplasia, particularly 2 to 4 anomalous vertebrae located between C5-T1 (80%). These consistent findings suggest a distinctive non-random association of malformations: M\u00fcllerian duct (MU) aplasia, renal (R) aplasia, and cervicothoracic somite (CS) dysplasia (MURCS). Identification of one component of the MURCS association suggests the presence of the other associated anomalies. A hypothesis for the embryogenic pathogenesis of the MURCS association is proposed which attributes the malformations to an alteration of the blastemas of the lower cervical-upper thoracic somites, arm buds, and pronephric ducts, all of which have an intimate spatial relationship at the end of the fourth week of fetal life. A presently unidentified teratogen may be one of the possible causes of the MURCS association on the basis of a lack of familial transmission, normal chromosomal studies, and the similar effects of a known teratogen (thalidomide) on the developing genitourinary tract."}
{"id": "PMID:469673", "title": "The spectrum of ABO hemolytic disease of the newborn infant.", "content": "A series of 1,704 infants of blood group O mothers have been studied to determine the relation between the degree of red cell sensitization and the cord hemoglobin and bilirubin concentrations. The infants with blood group A or B had significantly higher cord bilirubin and lower cord hemoglobin concentrations than the group O babies. Those infants whose red cells had the greatest evidence of sensitization had the highest bilirubin and lowest hemoglobin levels. The infants in whom no antibody was demonstrable on the red cells or in the red cell eluate also had significantly higher cord bilirubin and lower cord hemoglobin levels than the ABO compatible group; it is suggested that these infants had sufficient erythrocyte sensitization to produce mild hemolysis. ABO incompatibility represents a spectrum of hemolytic disease extending from those in which there is little laboratory evidence of erythrocyte sensitization, but evidence of hemolysis, to severe hemolytic disease in which erythrocyte sensitization is usually easily demonstrable.", "contents": "The spectrum of ABO hemolytic disease of the newborn infant. A series of 1,704 infants of blood group O mothers have been studied to determine the relation between the degree of red cell sensitization and the cord hemoglobin and bilirubin concentrations. The infants with blood group A or B had significantly higher cord bilirubin and lower cord hemoglobin concentrations than the group O babies. Those infants whose red cells had the greatest evidence of sensitization had the highest bilirubin and lowest hemoglobin levels. The infants in whom no antibody was demonstrable on the red cells or in the red cell eluate also had significantly higher cord bilirubin and lower cord hemoglobin levels than the ABO compatible group; it is suggested that these infants had sufficient erythrocyte sensitization to produce mild hemolysis. ABO incompatibility represents a spectrum of hemolytic disease extending from those in which there is little laboratory evidence of erythrocyte sensitization, but evidence of hemolysis, to severe hemolytic disease in which erythrocyte sensitization is usually easily demonstrable."}
{"id": "PMID:469674", "title": "Necrotizing enterocolitis in neonates fed human milk.", "content": "The incidence of necrotizing enterocolitis encountered in neonates fed only refrigerated human milk was comparable to that in infants fed milk and isotonic formula or isotonic formula alone. The infants fed human milk were significantly (P less than 0.05) smaller, less mature, had lower Apgar scores, and were fed later than the formula-fed infants. The mean age of onset and time between first feeding and onset of NEC was similar among the three groups. These data indicate that refrigerated human milk was not effective in lowering the incidence of NEC. Possible explanations for the occurrence of NEC in neonates fed human milk include: (1) the introduction of a pathogen via contaminated milk; (2) inadequate maternal antigenic stimulation by the neonatal gastrointestinal flora; and (3) adverse affects of storage on cell number and function.", "contents": "Necrotizing enterocolitis in neonates fed human milk. The incidence of necrotizing enterocolitis encountered in neonates fed only refrigerated human milk was comparable to that in infants fed milk and isotonic formula or isotonic formula alone. The infants fed human milk were significantly (P less than 0.05) smaller, less mature, had lower Apgar scores, and were fed later than the formula-fed infants. The mean age of onset and time between first feeding and onset of NEC was similar among the three groups. These data indicate that refrigerated human milk was not effective in lowering the incidence of NEC. Possible explanations for the occurrence of NEC in neonates fed human milk include: (1) the introduction of a pathogen via contaminated milk; (2) inadequate maternal antigenic stimulation by the neonatal gastrointestinal flora; and (3) adverse affects of storage on cell number and function."}
{"id": "PMID:469676", "title": "Therapeutic problems arising from the use of the intravenous route for drug administration.", "content": "The kinetics of drug delivery were studied under conditions of varying iv flow rates and varying sites of drug administration into an iv system. The iv sites and rates were selected on the basis of questionnaire data obtained from nursing personnel. The rate of drug delivery from the iv system was dependent upon the iv flow rate and site of injection of the drug into  the iv system. Under conditions of slow iv flow rates (3 ml/hour) there was the expected time delay required for the drug to begin to be infused but an unanticipated protracted time required for actual completion of the drug infusion. Calculation of theoretical drug blood levels based on the results of these iv studies revealed very different blood levels of drug at respective times, including different peak concentrations, depending on the iv flow rate and site at which the drug was injected into the system. Failure to appreciate the effects of slow iv rates and distal iv injection sites on actual rates of drug administration can adversely affect pharmacokinetic decisions and conceivably influence therapeutic success or failure.", "contents": "Therapeutic problems arising from the use of the intravenous route for drug administration. The kinetics of drug delivery were studied under conditions of varying iv flow rates and varying sites of drug administration into an iv system. The iv sites and rates were selected on the basis of questionnaire data obtained from nursing personnel. The rate of drug delivery from the iv system was dependent upon the iv flow rate and site of injection of the drug into  the iv system. Under conditions of slow iv flow rates (3 ml/hour) there was the expected time delay required for the drug to begin to be infused but an unanticipated protracted time required for actual completion of the drug infusion. Calculation of theoretical drug blood levels based on the results of these iv studies revealed very different blood levels of drug at respective times, including different peak concentrations, depending on the iv flow rate and site at which the drug was injected into the system. Failure to appreciate the effects of slow iv rates and distal iv injection sites on actual rates of drug administration can adversely affect pharmacokinetic decisions and conceivably influence therapeutic success or failure."}
{"id": "PMID:469677", "title": "Diagnosis, treatment, and follow-up of neonatal mepivacaine intoxication secondary to paracervical and pudendal blocks during labor.", "content": "Seven infants developed mepivacaine intoxication secondary to accidental injection during paracervical or pudental blocks or both. All presented with unexplained neonatal depression at birth, tonic seizures (often with apnea) within six hours, and characteristic neurologic findings. Twenty-four-hour urinary excretion produced 12.7 to 37.4 mg, exchange transfusions less than 1.02 to 3.5 mg, and gastric drainage or lavage or both 0.63 to 1.26 mg of mepivacaine. Thus promotion of urinary excretion is the treatment of choice. All six survivors are seizure free and neurologically and developmentally normal at one to 4 1/2 years. With early diagnosis and prevention of severe perinatal hypoxia, the prognosis from intoxication alone is very good.", "contents": "Diagnosis, treatment, and follow-up of neonatal mepivacaine intoxication secondary to paracervical and pudendal blocks during labor. Seven infants developed mepivacaine intoxication secondary to accidental injection during paracervical or pudental blocks or both. All presented with unexplained neonatal depression at birth, tonic seizures (often with apnea) within six hours, and characteristic neurologic findings. Twenty-four-hour urinary excretion produced 12.7 to 37.4 mg, exchange transfusions less than 1.02 to 3.5 mg, and gastric drainage or lavage or both 0.63 to 1.26 mg of mepivacaine. Thus promotion of urinary excretion is the treatment of choice. All six survivors are seizure free and neurologically and developmentally normal at one to 4 1/2 years. With early diagnosis and prevention of severe perinatal hypoxia, the prognosis from intoxication alone is very good."}
{"id": "PMID:469678", "title": "Adaptation of siblings to childhood malignancy.", "content": "To explore the impact of childhood cancer, school-aged patients and their healthy siblings from 71 families were given one or more of the following psychologic tests: the Piers-Harris Children's Self-Concept Scale, the Bene-Anthony Family Relations Test, and the Thematic Apperception Test. The siblings showed even more distress than the patients in the areas of perceived social isolation, perception of their parents as overindulgent and overprotective of the sick child, fear of confronting family members with negative feelings, and concern with failure (older siblings only). In other areas, such as anxiety and vulnerability to illness and injury, the patients' and siblings' experience appeared very similar. These results indicate that siblings, like patients, experience severe stress when confronted with a chronic, life-threatening illness such as cancer. In recognizing and attending to their needs, one can practice primary prevention in pediatrics by facilitating their healthy adaptation to a very difficult situation.", "contents": "Adaptation of siblings to childhood malignancy. To explore the impact of childhood cancer, school-aged patients and their healthy siblings from 71 families were given one or more of the following psychologic tests: the Piers-Harris Children's Self-Concept Scale, the Bene-Anthony Family Relations Test, and the Thematic Apperception Test. The siblings showed even more distress than the patients in the areas of perceived social isolation, perception of their parents as overindulgent and overprotective of the sick child, fear of confronting family members with negative feelings, and concern with failure (older siblings only). In other areas, such as anxiety and vulnerability to illness and injury, the patients' and siblings' experience appeared very similar. These results indicate that siblings, like patients, experience severe stress when confronted with a chronic, life-threatening illness such as cancer. In recognizing and attending to their needs, one can practice primary prevention in pediatrics by facilitating their healthy adaptation to a very difficult situation."}
{"id": "PMID:469694", "title": "Is the elderly primipara really at high risk?", "content": "During a five-year period, from 1970 through 1974, 26.776 deliveries occurred in our department, of which 55 (0.6%) were in elderly primiparae (EP). This group was compared with two control groups. The first comprised 97 women aged 30--34 years, and the second one, 250 women between 20 and 29 years of age. The parameters investigated were: The gynecologic past history, the course of the present pregnancy, labor, delivery and fetal outcome. In most of the cases, no significant differences were found between the EP group and the control groups. A striking difference was observed in the rate of cesarean sections, which was 49.1% in the EP group, as against only 2.3% in the age group 20--29 years. It may be concluded that by more liberal use of cesarean sections, available means of antenatal care, and prompt intervention in cases of postmaturity and prolonged labor, one can reduce the maternal and fetal morbidity and mortality, and that the EP, although a group at risk, has nowadays a better outlook for both mother and fetus.", "contents": "Is the elderly primipara really at high risk? During a five-year period, from 1970 through 1974, 26.776 deliveries occurred in our department, of which 55 (0.6%) were in elderly primiparae (EP). This group was compared with two control groups. The first comprised 97 women aged 30--34 years, and the second one, 250 women between 20 and 29 years of age. The parameters investigated were: The gynecologic past history, the course of the present pregnancy, labor, delivery and fetal outcome. In most of the cases, no significant differences were found between the EP group and the control groups. A striking difference was observed in the rate of cesarean sections, which was 49.1% in the EP group, as against only 2.3% in the age group 20--29 years. It may be concluded that by more liberal use of cesarean sections, available means of antenatal care, and prompt intervention in cases of postmaturity and prolonged labor, one can reduce the maternal and fetal morbidity and mortality, and that the EP, although a group at risk, has nowadays a better outlook for both mother and fetus."}
{"id": "PMID:469699", "title": "Relationships between locus of control, trait anxiety, and state anxiety: an interactionist perspective.", "content": "This study examined the relationships among generalized expectancy for internal or external control, situational expectancy of control over aversive stimuli, trait anxiety level, and state anxiety reactivity. Seventy-two male subjects, selected from the upper and lower halves of the distributions of scores from the I-E and STAI A-Trait Scales, were randomly assigned to one of three conditions of expectancy of control over electric shock. Repeated measures of state anxiety were taken at three points in the experiment, and subjects were asked to rate the degree of control they expected to have in avoiding shocks. Results, based upon analyses of variance, were interpreted as strongly supportive of the use of the interactionist model in understanding the manner in which personality trait and situational variables interact in the determination of subject expectancies of control and anxiety reactivity.", "contents": "Relationships between locus of control, trait anxiety, and state anxiety: an interactionist perspective. This study examined the relationships among generalized expectancy for internal or external control, situational expectancy of control over aversive stimuli, trait anxiety level, and state anxiety reactivity. Seventy-two male subjects, selected from the upper and lower halves of the distributions of scores from the I-E and STAI A-Trait Scales, were randomly assigned to one of three conditions of expectancy of control over electric shock. Repeated measures of state anxiety were taken at three points in the experiment, and subjects were asked to rate the degree of control they expected to have in avoiding shocks. Results, based upon analyses of variance, were interpreted as strongly supportive of the use of the interactionist model in understanding the manner in which personality trait and situational variables interact in the determination of subject expectancies of control and anxiety reactivity."}
{"id": "PMID:469698", "title": "Investigation of some cord blood components.", "content": "The practical result borne by our investigations has been the introduction of routine estimation of cord blood total protein. In every case when the concentration is below the 10 percentile value, the clinicians are warned. Further catamnestic studies are needed to judge the values of cord blood cholesterol estimations in screening for familial hyperlipoproteinemias. The hypercholeterolemic infants of the present series, who are 2-3 years old at present, as well as their families must be subjected to repeat analysis. The change in opposite directions of cord blood total protein and cholesterol levels with the advance of gestation was a remarkable finding. This correlation indicates a close interaction between protein and lipid metabolism. Less promising results have been obtained in investigations of the cord blood calcium and magnesium levels, but final conclusions can only be drawn after repeat analyses. Introduction of the routine estimation of these two parameters does not seem necessary at present. Besides clinical data, biochemical parameters have also been found suitable for differentiating small-for-date infants from true prematures. The functional maturity of these infants was shown by the fact that their different cord blood components were normal.", "contents": "Investigation of some cord blood components. The practical result borne by our investigations has been the introduction of routine estimation of cord blood total protein. In every case when the concentration is below the 10 percentile value, the clinicians are warned. Further catamnestic studies are needed to judge the values of cord blood cholesterol estimations in screening for familial hyperlipoproteinemias. The hypercholeterolemic infants of the present series, who are 2-3 years old at present, as well as their families must be subjected to repeat analysis. The change in opposite directions of cord blood total protein and cholesterol levels with the advance of gestation was a remarkable finding. This correlation indicates a close interaction between protein and lipid metabolism. Less promising results have been obtained in investigations of the cord blood calcium and magnesium levels, but final conclusions can only be drawn after repeat analyses. Introduction of the routine estimation of these two parameters does not seem necessary at present. Besides clinical data, biochemical parameters have also been found suitable for differentiating small-for-date infants from true prematures. The functional maturity of these infants was shown by the fact that their different cord blood components were normal."}
{"id": "PMID:469700", "title": "Interpersonal aggression as a function of subject's sex, subject's sex role identification, opponent's sex, and degree of provocation.", "content": "Ninety-five undergraduates served as subjects in an experiment where they could administer electric shock to an opponent and receive the same from an opponent. The independent variables were subject's sex, subject's sex role identification (as measured by the Bem Sex Role Inventory), opponent's sex, as well as degree of provocation from the opponent. Aggression was defined as level of shock chosen by the subject for the opponent. The results indicated that masculine subjects facing a male opponent were more aggressive than individuals of other sex role identifications whether or not they were provoked. It was also found that masculine males were more aggressive than other males or anyof the females. Furthermore, opponent's sex influenced the males' aggressiveness but had no effect on the degree of aggression in females. Finally, aggression increased in all subjects following increases in provocation. The results are discussed in terms of their implications for pyschological androgyny.", "contents": "Interpersonal aggression as a function of subject's sex, subject's sex role identification, opponent's sex, and degree of provocation. Ninety-five undergraduates served as subjects in an experiment where they could administer electric shock to an opponent and receive the same from an opponent. The independent variables were subject's sex, subject's sex role identification (as measured by the Bem Sex Role Inventory), opponent's sex, as well as degree of provocation from the opponent. Aggression was defined as level of shock chosen by the subject for the opponent. The results indicated that masculine subjects facing a male opponent were more aggressive than individuals of other sex role identifications whether or not they were provoked. It was also found that masculine males were more aggressive than other males or anyof the females. Furthermore, opponent's sex influenced the males' aggressiveness but had no effect on the degree of aggression in females. Finally, aggression increased in all subjects following increases in provocation. The results are discussed in terms of their implications for pyschological androgyny."}
{"id": "PMID:469701", "title": "Interpersonal touching and orality.", "content": "Sixty-five undergraduates were assigned to guide a blindfolded confederate through a floor maze. The frequency of touching the confederate's skin and the length of time required to complete the trip through the maze were recorded. Each subject was also given a self-administered Rorschach test; an independent count was made of the number of oral responses given. Orality was positively related to touching behavior (r=.39, p=.001) and with speed of guiding the confederate through the maze (r=.31, p=.01).", "contents": "Interpersonal touching and orality. Sixty-five undergraduates were assigned to guide a blindfolded confederate through a floor maze. The frequency of touching the confederate's skin and the length of time required to complete the trip through the maze were recorded. Each subject was also given a self-administered Rorschach test; an independent count was made of the number of oral responses given. Orality was positively related to touching behavior (r=.39, p=.001) and with speed of guiding the confederate through the maze (r=.31, p=.01)."}
{"id": "PMID:469702", "title": "A data-based typology of depressed adolescents.", "content": "To develop a data-based classification of depressive adolescents, a face valid depression scale for adolescents was applied to MMPI protocols of 212 adolescent psychiatric patients of ages 12 to 18, to identify a depressive subsample which turned out to have 36 females and 10 males. Complete linkage cluster analysis was applied to this subsample and three hierachically organized depressive groups were obtained: \"Restless\" and \"Socially Frustrated\", which joined into a higher order group, and \"Endogenous\". Discriminant function analysis was applied for differentiating and cross-validating the obtained groups.", "contents": "A data-based typology of depressed adolescents. To develop a data-based classification of depressive adolescents, a face valid depression scale for adolescents was applied to MMPI protocols of 212 adolescent psychiatric patients of ages 12 to 18, to identify a depressive subsample which turned out to have 36 females and 10 males. Complete linkage cluster analysis was applied to this subsample and three hierachically organized depressive groups were obtained: \"Restless\" and \"Socially Frustrated\", which joined into a higher order group, and \"Endogenous\". Discriminant function analysis was applied for differentiating and cross-validating the obtained groups."}
{"id": "PMID:469703", "title": "Conception and representation of space in human figure drawings by schizophrenic and normal subjects.", "content": "Seventy hospitalized schizophrenic adults, and 71 normal subjects were given a test consisting of six human figure drawings, arranged in a thematic sequence from a self-portrait to a drawing of a person. The structural components of each human figure were scored and analyzed in an attempt to validate their projective character and to examine influence of psychopathology on cognition. Findings indicated similar constructional trends for both groups, with differences particular to sex, rather than a diagnostic category. It was concluded that conception and representation of space and its configurations will not be influenced by psychopathological processes.", "contents": "Conception and representation of space in human figure drawings by schizophrenic and normal subjects. Seventy hospitalized schizophrenic adults, and 71 normal subjects were given a test consisting of six human figure drawings, arranged in a thematic sequence from a self-portrait to a drawing of a person. The structural components of each human figure were scored and analyzed in an attempt to validate their projective character and to examine influence of psychopathology on cognition. Findings indicated similar constructional trends for both groups, with differences particular to sex, rather than a diagnostic category. It was concluded that conception and representation of space and its configurations will not be influenced by psychopathological processes."}
{"id": "PMID:469704", "title": "Symptomatology of depression in adolescence.", "content": "The symptomatology and dimensions of depression in adolescence were studied using the MMPI protocols of 212 adolescent psychiatric patients. A Face Valid Depression Scale for Adolescents was judgmentally developed by five child psychiatrists and psychologists. It included symptoms common to depressive adults and adolescents as well as symptoms peculiar to adolescents, which was confirmed by comparison with adult depression scales. Factor analysis of the scale yielded the following six factors: lack of self-confidence, social abandonment, loss of interests, sadness, somatic symptoms, and acting-out. The second and sixth factors appeared to be particularly characteristic of adolescents.", "contents": "Symptomatology of depression in adolescence. The symptomatology and dimensions of depression in adolescence were studied using the MMPI protocols of 212 adolescent psychiatric patients. A Face Valid Depression Scale for Adolescents was judgmentally developed by five child psychiatrists and psychologists. It included symptoms common to depressive adults and adolescents as well as symptoms peculiar to adolescents, which was confirmed by comparison with adult depression scales. Factor analysis of the scale yielded the following six factors: lack of self-confidence, social abandonment, loss of interests, sadness, somatic symptoms, and acting-out. The second and sixth factors appeared to be particularly characteristic of adolescents."}
{"id": "PMID:469705", "title": "Differences in the severity of disturbance of behaviors in children receiving inpatient and outpatient psychiatric treatment.", "content": "This study was conducted to determine if there are differences in the severity of disturbance of behaviors exhibited by children receiving inpatient psychiatric treatment and those receiving outpatient psychiatric treatment based on their parents' judgments of the frequency of occurrence of those behaviors. The same behaviors were found to exist in both treatment groups, but the severity of disturbance of those behaviors in the inpatient sample was significantly greater than in the outpatient sample. The scale used in this study was found to be a valid and useful instrument for predicting inpatient and outpatient status of children. Pratice and research implications of this study are also discussed.", "contents": "Differences in the severity of disturbance of behaviors in children receiving inpatient and outpatient psychiatric treatment. This study was conducted to determine if there are differences in the severity of disturbance of behaviors exhibited by children receiving inpatient psychiatric treatment and those receiving outpatient psychiatric treatment based on their parents' judgments of the frequency of occurrence of those behaviors. The same behaviors were found to exist in both treatment groups, but the severity of disturbance of those behaviors in the inpatient sample was significantly greater than in the outpatient sample. The scale used in this study was found to be a valid and useful instrument for predicting inpatient and outpatient status of children. Pratice and research implications of this study are also discussed."}
{"id": "PMID:469706", "title": "Factor structure and construct validity of \"what I think and feel\": The Revised Children's Manifest Anxiety Scale.", "content": "Responses to the Revised-CMAS of 329 children from grades 1 through 12 were factor analyzed. Consistent with current multidimensional theories of anxiety, three primary anxiety factors emerged, supporting the construct validity of the new scale. Implications for interpretation of the revised scale are discussed.", "contents": "Factor structure and construct validity of \"what I think and feel\": The Revised Children's Manifest Anxiety Scale. Responses to the Revised-CMAS of 329 children from grades 1 through 12 were factor analyzed. Consistent with current multidimensional theories of anxiety, three primary anxiety factors emerged, supporting the construct validity of the new scale. Implications for interpretation of the revised scale are discussed."}
{"id": "PMID:469707", "title": "Effects of dogmatism on state anxiety during the analysis and synthesis of new beliefs.", "content": "Investigated the concept of dogmatism as a defense mechanism and the role of threat in the synthesis of new beliefs by examining the effects of dogmatism on changes in state anxiety (A-State) during the analysis and synthesis of new beliefs. Sixty female college students were selected on the basis of extreme scores on the Dogmatism Scale and the trait anxiety (A-Trait) scale of the State-Trait Anxiety Inventory to work on a task requiring the analysis and synthesis of new beliefs. In support of Rokeach's theory, high dogmatics displayed no change in A-State from the analysis to the synthesis period of the task, while low dogmatics exhibited a significant decline in A-State between the two periods. The clinical implications of these findings were discussed in terms of the role of dogmatism in the processing of personality interpretations and test feedback.", "contents": "Effects of dogmatism on state anxiety during the analysis and synthesis of new beliefs. Investigated the concept of dogmatism as a defense mechanism and the role of threat in the synthesis of new beliefs by examining the effects of dogmatism on changes in state anxiety (A-State) during the analysis and synthesis of new beliefs. Sixty female college students were selected on the basis of extreme scores on the Dogmatism Scale and the trait anxiety (A-Trait) scale of the State-Trait Anxiety Inventory to work on a task requiring the analysis and synthesis of new beliefs. In support of Rokeach's theory, high dogmatics displayed no change in A-State from the analysis to the synthesis period of the task, while low dogmatics exhibited a significant decline in A-State between the two periods. The clinical implications of these findings were discussed in terms of the role of dogmatism in the processing of personality interpretations and test feedback."}
{"id": "PMID:469708", "title": "MMPI ratings of suicide risk.", "content": "Among past attempts to use MMPI data to predict suicidal behavior, there has been a lack of research on the ability of clinicians to identify MMPI profiles of suicidal persons. In this study, the MMPI profiles of 20 male psychiatric patients who committed suicide and the MMPI profiles of 20 male patients who did not attempt or commit suicide were presented to six clinical psychologists with expertise in MMPI interpretation. The clinicians were asked to classify each MMPI profile as coming from a patient who did or did not later commit suicide, and to rate eight variables thought to be relevant to the assessment of suicide risk. Data analysis revealed that the clinicians could not identify suicide and nonsuicide patients from their MMPI profiles. Furthermore, the ratings of the eight suicide variable did not differentiate suicide and nonsuicide patients.", "contents": "MMPI ratings of suicide risk. Among past attempts to use MMPI data to predict suicidal behavior, there has been a lack of research on the ability of clinicians to identify MMPI profiles of suicidal persons. In this study, the MMPI profiles of 20 male psychiatric patients who committed suicide and the MMPI profiles of 20 male patients who did not attempt or commit suicide were presented to six clinical psychologists with expertise in MMPI interpretation. The clinicians were asked to classify each MMPI profile as coming from a patient who did or did not later commit suicide, and to rate eight variables thought to be relevant to the assessment of suicide risk. Data analysis revealed that the clinicians could not identify suicide and nonsuicide patients from their MMPI profiles. Furthermore, the ratings of the eight suicide variable did not differentiate suicide and nonsuicide patients."}
{"id": "PMID:469709", "title": "Jo-Jo, Josephine, and Joanne: a study of multiple personality by means of the Rorschach Test.", "content": "A comparison of three Rorschach protocols of a multiple personality case was presented. Basic features of the personality structures were examined with respect to the psychogram profiles. The evolution of the original personality into the successive ones was assessed both in terms of inter- and intra-protocol variations and thematic analysis. The implications of the Rorschach test findings for the treatment and theory of multiple personality syndrome were discussed.", "contents": "Jo-Jo, Josephine, and Joanne: a study of multiple personality by means of the Rorschach Test. A comparison of three Rorschach protocols of a multiple personality case was presented. Basic features of the personality structures were examined with respect to the psychogram profiles. The evolution of the original personality into the successive ones was assessed both in terms of inter- and intra-protocol variations and thematic analysis. The implications of the Rorschach test findings for the treatment and theory of multiple personality syndrome were discussed."}
{"id": "PMID:469712", "title": "Synaptic drive and impulse generation in ganglion cells of turtle retina.", "content": "1. Light reponses and electrical constants of ganglion cells in the retina of the turtle were examined by intracellular recording in eyecup preparations. 2. In 'on', 'off', and 'on/off' cells, the impulses produced by illumination of the centre of the receptive field arose from slow synaptic depolarizations. The ganglion cells also exhibited inhibitory synaptic potentials. 3. The synaptic depolarization evoked by a step change in light intensity rose more slowly than the response of the cones in which the excitation originated, and the depolarization then declined in spite of a well maintained cone response. This behaviour is consistent with the notion advanced previously that, during transmission to ganglion cells, receptor signals are relayed through the equivalent of a bandpass filter. 4. The e.p.s.p.s evoked by light grew when the membrane was hyperpolarized by injected current and decreased when the membrane was depolarized. The i.p.s.p.s reversed at a level slightly negative to the resting potential in darkness. 5. In neither 'on' nor 'off' ganglion cells did the synaptic potentials evoked by step changes in illumination show the hyperpolarizing phases expected of a linear filter. The absence of hyperpolarizations is consistent with a rectification which permits transmission of depolarizations but not hyperpolarizations from bipolar to ganglion cells. 6. In darkness the membrane potential of some ganglion cells showed random depolarizations which brought the potential near the threshold for impulse generation. 7. With very small spots in the receptive field centre the 'on' responses of ganglion cells to flashes and steps of light grew approximately linearly with stimulus intensity. The step reponse was not, however, related to the flash response by superposition. Larger spots in the field centre gave responses which grew non-linearly with the intensity of even dim stimuli. 8 Depolarizing current passed through the recording electrode elicited a repetitive discharge of impulses. The frequency of firing increased linearly with current strength above a rheobase value of about 10(-10) A. Accommodation occurred during steady currents, the main decline taking place with a time constant of about 15 msec. 9. Strength-latency measurements and bridge records of ganglion cell charging by constant currents gave time constants of 10--20 msec and input resistances of 100--150 M omega.", "contents": "Synaptic drive and impulse generation in ganglion cells of turtle retina. 1. Light reponses and electrical constants of ganglion cells in the retina of the turtle were examined by intracellular recording in eyecup preparations. 2. In 'on', 'off', and 'on/off' cells, the impulses produced by illumination of the centre of the receptive field arose from slow synaptic depolarizations. The ganglion cells also exhibited inhibitory synaptic potentials. 3. The synaptic depolarization evoked by a step change in light intensity rose more slowly than the response of the cones in which the excitation originated, and the depolarization then declined in spite of a well maintained cone response. This behaviour is consistent with the notion advanced previously that, during transmission to ganglion cells, receptor signals are relayed through the equivalent of a bandpass filter. 4. The e.p.s.p.s evoked by light grew when the membrane was hyperpolarized by injected current and decreased when the membrane was depolarized. The i.p.s.p.s reversed at a level slightly negative to the resting potential in darkness. 5. In neither 'on' nor 'off' ganglion cells did the synaptic potentials evoked by step changes in illumination show the hyperpolarizing phases expected of a linear filter. The absence of hyperpolarizations is consistent with a rectification which permits transmission of depolarizations but not hyperpolarizations from bipolar to ganglion cells. 6. In darkness the membrane potential of some ganglion cells showed random depolarizations which brought the potential near the threshold for impulse generation. 7. With very small spots in the receptive field centre the 'on' responses of ganglion cells to flashes and steps of light grew approximately linearly with stimulus intensity. The step reponse was not, however, related to the flash response by superposition. Larger spots in the field centre gave responses which grew non-linearly with the intensity of even dim stimuli. 8 Depolarizing current passed through the recording electrode elicited a repetitive discharge of impulses. The frequency of firing increased linearly with current strength above a rheobase value of about 10(-10) A. Accommodation occurred during steady currents, the main decline taking place with a time constant of about 15 msec. 9. Strength-latency measurements and bridge records of ganglion cell charging by constant currents gave time constants of 10--20 msec and input resistances of 100--150 M omega."}
{"id": "PMID:469713", "title": "Cardiovascular and antidiuretic effects of central prostaglandin E2.", "content": "1. Unanaesthetized normal and water-loaded rats were tested for cardiovascular and antidiuretic responses to intracerebroventricular (I.V.T.) injections of prostaglandin E2 (PGE2). 2. I.V.T. injections of PGE2 produced tachycardia and a slow rise in blood pressure that reached a peak in 15 min and lasted up to 2 hr. 3. Intravenous pre-treatment with phenoxybenzamine, an alpha-adrenergic blocker, inhibited pressor but not heart rate responses to I.V.T. PGE2. Pre-treatment with propranalol, a beta-adrenergic blocker, inhibited tachycarcia but not pressor responses to I.V.T. PGE2. Atropine attenuated heart rate and blood pressure responses when higher doses of PGE2 were tested. 4. In water-loaded rats, I.V.T. PGE2 produced antidiuretic effects suggesting release of antidiuretic hormone in subpressor amounts. Antidiuretic but not cardiovascular responses to I.V.T. PGE2 were abolished by median eminence lesions. 5. Intravenous PGE2 infusions produced a decrease in blood pressure and no antidiuretic effects, indicating that both responses were centrally mediated. 6. The results indicate that I.V.T. PGE2 produces pressor and tachycardia responses in the unanaesthetized rat which are mediated primarily by centrally mediated sympathetic outflow.", "contents": "Cardiovascular and antidiuretic effects of central prostaglandin E2. 1. Unanaesthetized normal and water-loaded rats were tested for cardiovascular and antidiuretic responses to intracerebroventricular (I.V.T.) injections of prostaglandin E2 (PGE2). 2. I.V.T. injections of PGE2 produced tachycardia and a slow rise in blood pressure that reached a peak in 15 min and lasted up to 2 hr. 3. Intravenous pre-treatment with phenoxybenzamine, an alpha-adrenergic blocker, inhibited pressor but not heart rate responses to I.V.T. PGE2. Pre-treatment with propranalol, a beta-adrenergic blocker, inhibited tachycarcia but not pressor responses to I.V.T. PGE2. Atropine attenuated heart rate and blood pressure responses when higher doses of PGE2 were tested. 4. In water-loaded rats, I.V.T. PGE2 produced antidiuretic effects suggesting release of antidiuretic hormone in subpressor amounts. Antidiuretic but not cardiovascular responses to I.V.T. PGE2 were abolished by median eminence lesions. 5. Intravenous PGE2 infusions produced a decrease in blood pressure and no antidiuretic effects, indicating that both responses were centrally mediated. 6. The results indicate that I.V.T. PGE2 produces pressor and tachycardia responses in the unanaesthetized rat which are mediated primarily by centrally mediated sympathetic outflow."}
{"id": "PMID:469714", "title": "An analysis of the wave forms of photoreceptor potentials in the retina of the chephalopod Sepiola atlantica.", "content": "1. Simultaneous intracellular (Vi) and extracellular (Ve) recordings have been made from photoreceptor cells in the retina of the cephalopod Sepiola atlantica. 2. The depolarization of the distal (rhodopsin containing) membrane (Vm) was derived from the Vi and Ve records. 3. All three potentials had very similar time courses in response to short flashes of low intensity but the Vi and Vm responses were much greater in amplitude. 4. At high intensities the amplitudes of Vi and Ve were similar but the wave forms were quite different and it was postulated that a voltage-sensitive potassium conductance change in the proximal membranes of the cell was mainly reponsible for the observed differences. 5. The wave forms of the three responses following long (500 msec) flashes were quite different at all intensities tested. At high intensities there was a slow sag phase in the Vi response, that was mirrored by a slow rise phase in the Ve responses. The reconstructed Vm response was flat during this period of change in the other two responses. 6. The recoveries to the base line of all responses following both long and short flashes were similar in that at high intensities they consisted of at least two phases. The fast phase had a time constant less than 1 sec and the slow component had a time constant of greater than 10 sec. 7. The maximum rates of change of voltage (dV/dt) in both short and long flash responses were linear over a much wider range of intensity values than were the response amplitudes and the rates continued to increase after the peak response amplitude had saturated. 8. The characteristics of the cephalopod responses were compared with those from vertebrate rods and cones and several marked similarities were found.", "contents": "An analysis of the wave forms of photoreceptor potentials in the retina of the chephalopod Sepiola atlantica. 1. Simultaneous intracellular (Vi) and extracellular (Ve) recordings have been made from photoreceptor cells in the retina of the cephalopod Sepiola atlantica. 2. The depolarization of the distal (rhodopsin containing) membrane (Vm) was derived from the Vi and Ve records. 3. All three potentials had very similar time courses in response to short flashes of low intensity but the Vi and Vm responses were much greater in amplitude. 4. At high intensities the amplitudes of Vi and Ve were similar but the wave forms were quite different and it was postulated that a voltage-sensitive potassium conductance change in the proximal membranes of the cell was mainly reponsible for the observed differences. 5. The wave forms of the three responses following long (500 msec) flashes were quite different at all intensities tested. At high intensities there was a slow sag phase in the Vi response, that was mirrored by a slow rise phase in the Ve responses. The reconstructed Vm response was flat during this period of change in the other two responses. 6. The recoveries to the base line of all responses following both long and short flashes were similar in that at high intensities they consisted of at least two phases. The fast phase had a time constant less than 1 sec and the slow component had a time constant of greater than 10 sec. 7. The maximum rates of change of voltage (dV/dt) in both short and long flash responses were linear over a much wider range of intensity values than were the response amplitudes and the rates continued to increase after the peak response amplitude had saturated. 8. The characteristics of the cephalopod responses were compared with those from vertebrate rods and cones and several marked similarities were found."}
{"id": "PMID:469715", "title": "Facilitation of the lordosis reflex of female rats from the ventromedial nucleus of the hypothalamus.", "content": "1. Effects of electrical stimulation of hypothalamic ventromedial nucleus (v.m.n.) on the lordosis reflex of female rats were examined in ovariectomized and oestrogen-primed animals with chronically implanted electrodes. 2. Lordosis triggered either by manual cutaneous stimulation or by male mounting, was facilitated by electrical stimulation of the v.m.n. 3 A gradual increase in lordosis performance followed a relatively long period of stimulation; never less than 15 min and usually about 1 hr of stimulation was necessary for maximum facilitation. Following the termination of stimulation, the performance returned gradually to the control level during a 5--8 hr period. 4. The optimal frequency of stimulation was between 10 and 30 Hz. Threshold for effective facilitation was, on the average, 12.5 microA. 5. Stimulation tended to induce larger facilitation when applied to the lateral side of v.m.n. 6. Pre-treatment with oestrogen was necessary to obtain facilitation by v.m.n. stimulation. The threshold dosage of oestrogen was 2.5 microgram per animal. 7. Stimulation was effective in adrenalectomized rats, in dexamethasone-primed animals, and in rats pre-treated with exogenous progesterone. Thus, adrenal prodesterone release is not required for the v.m.n. facilitation of lordosis. 8. Medial preoptic stimulation with the same parameters suppressed the lordosis reflex. 9. The v.m.n. participates in the control of lordosis by a facilitatory output. The delay before facilitation implies that the v.m.n. is not in the direct reflex-arc for the execution of lordosis. Rather, a summation or interaction process with an unusually long time course is involved.", "contents": "Facilitation of the lordosis reflex of female rats from the ventromedial nucleus of the hypothalamus. 1. Effects of electrical stimulation of hypothalamic ventromedial nucleus (v.m.n.) on the lordosis reflex of female rats were examined in ovariectomized and oestrogen-primed animals with chronically implanted electrodes. 2. Lordosis triggered either by manual cutaneous stimulation or by male mounting, was facilitated by electrical stimulation of the v.m.n. 3 A gradual increase in lordosis performance followed a relatively long period of stimulation; never less than 15 min and usually about 1 hr of stimulation was necessary for maximum facilitation. Following the termination of stimulation, the performance returned gradually to the control level during a 5--8 hr period. 4. The optimal frequency of stimulation was between 10 and 30 Hz. Threshold for effective facilitation was, on the average, 12.5 microA. 5. Stimulation tended to induce larger facilitation when applied to the lateral side of v.m.n. 6. Pre-treatment with oestrogen was necessary to obtain facilitation by v.m.n. stimulation. The threshold dosage of oestrogen was 2.5 microgram per animal. 7. Stimulation was effective in adrenalectomized rats, in dexamethasone-primed animals, and in rats pre-treated with exogenous progesterone. Thus, adrenal prodesterone release is not required for the v.m.n. facilitation of lordosis. 8. Medial preoptic stimulation with the same parameters suppressed the lordosis reflex. 9. The v.m.n. participates in the control of lordosis by a facilitatory output. The delay before facilitation implies that the v.m.n. is not in the direct reflex-arc for the execution of lordosis. Rather, a summation or interaction process with an unusually long time course is involved."}
{"id": "PMID:469716", "title": "Deficit in the lordosis reflex of female rats caused by lesions in the ventromedial nucleus of the hypothalamus.", "content": "1. The effect of electrolytic lesions of the ventromedial nucleus of the hypothalamus (v.m.n.) on the lordosis reflex has been investigated on ovariectomized female rats. Lesions were made through chronically implanted platinum-iridium electrodes. 2. V.m.n. lesions did not disrupt lordosis immediately, but induced a gradual decline in the reflex. Lordosis performance reached it minimum no less than 12 hr after the lesion, and typically after 36--60 hr. 3. The magnitude of the lordosis deficit was related to the amount of v.m.n. damage. Destruction of other hypothalamic regions was without appreciable relation to the deficit. Within v.m.n., lesion size in the lateral, but not medial portion was significantly correlated with lordosis deficit. 4. Because of the slow time courses of v.m.n. lesions and stimulation (Pfaff & Sakuma, 1978) effects, it is postulated that the v.m.n. is not part of the direct reflex-arc for lordosis. Rather, neurones in v.m.n. are likely to exert a tonic hormone-dependent bias on brain stem reflex paths for this behaviour.", "contents": "Deficit in the lordosis reflex of female rats caused by lesions in the ventromedial nucleus of the hypothalamus. 1. The effect of electrolytic lesions of the ventromedial nucleus of the hypothalamus (v.m.n.) on the lordosis reflex has been investigated on ovariectomized female rats. Lesions were made through chronically implanted platinum-iridium electrodes. 2. V.m.n. lesions did not disrupt lordosis immediately, but induced a gradual decline in the reflex. Lordosis performance reached it minimum no less than 12 hr after the lesion, and typically after 36--60 hr. 3. The magnitude of the lordosis deficit was related to the amount of v.m.n. damage. Destruction of other hypothalamic regions was without appreciable relation to the deficit. Within v.m.n., lesion size in the lateral, but not medial portion was significantly correlated with lordosis deficit. 4. Because of the slow time courses of v.m.n. lesions and stimulation (Pfaff & Sakuma, 1978) effects, it is postulated that the v.m.n. is not part of the direct reflex-arc for lordosis. Rather, neurones in v.m.n. are likely to exert a tonic hormone-dependent bias on brain stem reflex paths for this behaviour."}
{"id": "PMID:469717", "title": "Reciprocal transplantations between the optic tectum and the cerebellum in adult goldfish.", "content": "1. The topographic pattern of re-established visual projections after a reciprocal transplantation between tectal and cerebellar tissues was studied in adult goldfish. 2. A rectangular tissue was dissected from the left tectum, and a similar piece from the cerebellum in the same fish. The cerebellar piece was rotated by either 180 or 0 degrees around the dorsoventral axis, and transplanted into the tectum in place of the tectal piece. This tectal tissue was likewise grafted into the cerebellum after either 180 or 0 degrees rotation in the same fish. 3. The tectal graft disappeared from the cerebellum within 2 months after surgery. The operated cerebellum showed a remarkable capability for healing its excised part. No visual responses were recorded from the cerebellum. 4. The cerebellar grafts remained in place within the operated tectum in twenty fish. In fifteen of these fish, tested 3 or 4 months after surgery, the cerebellar grafts did not give any visual responses, unlike the surrounding responsive area of the tectum. These fish showed a partial scotoma in the central area of the visual field, which corresponded to the unresponsive transplanted area of the tectum. Autoradiographic examination after intraocular injection of L-[3H]proline showed that these cerebellar grafts did not contain any noticeable label, in contrast to the extensively labelled surrounding tectal tissues. 5. Sporadic visual responses were recorded from deep layers in the transplanted area of the tectum in five of the twenty fish at early post-operative periods. The receptive fields of these responses were distributed in a correct retinotopic order, regardless of whether the cerebellar tissue had been rotated by either 180 or 0 degrees. Autoradiographic examination, however, revealed that these cerebellar grafts were not invaded by regenerating optic fibres. Instead, they bypassed the interposed cerebellar tissue by making detours beneath the graft on the way towards their appropriate target zones within the tectal tissue. 6. This selective avoidance of a foreign (cerebellar) tissue and the orderly reinnervation of the proper tectal tissue by regenerating optic fibres provide us with further evidence for neuronal specificity.", "contents": "Reciprocal transplantations between the optic tectum and the cerebellum in adult goldfish. 1. The topographic pattern of re-established visual projections after a reciprocal transplantation between tectal and cerebellar tissues was studied in adult goldfish. 2. A rectangular tissue was dissected from the left tectum, and a similar piece from the cerebellum in the same fish. The cerebellar piece was rotated by either 180 or 0 degrees around the dorsoventral axis, and transplanted into the tectum in place of the tectal piece. This tectal tissue was likewise grafted into the cerebellum after either 180 or 0 degrees rotation in the same fish. 3. The tectal graft disappeared from the cerebellum within 2 months after surgery. The operated cerebellum showed a remarkable capability for healing its excised part. No visual responses were recorded from the cerebellum. 4. The cerebellar grafts remained in place within the operated tectum in twenty fish. In fifteen of these fish, tested 3 or 4 months after surgery, the cerebellar grafts did not give any visual responses, unlike the surrounding responsive area of the tectum. These fish showed a partial scotoma in the central area of the visual field, which corresponded to the unresponsive transplanted area of the tectum. Autoradiographic examination after intraocular injection of L-[3H]proline showed that these cerebellar grafts did not contain any noticeable label, in contrast to the extensively labelled surrounding tectal tissues. 5. Sporadic visual responses were recorded from deep layers in the transplanted area of the tectum in five of the twenty fish at early post-operative periods. The receptive fields of these responses were distributed in a correct retinotopic order, regardless of whether the cerebellar tissue had been rotated by either 180 or 0 degrees. Autoradiographic examination, however, revealed that these cerebellar grafts were not invaded by regenerating optic fibres. Instead, they bypassed the interposed cerebellar tissue by making detours beneath the graft on the way towards their appropriate target zones within the tectal tissue. 6. This selective avoidance of a foreign (cerebellar) tissue and the orderly reinnervation of the proper tectal tissue by regenerating optic fibres provide us with further evidence for neuronal specificity."}
{"id": "PMID:469718", "title": "Voltage-clamp analysis of the potassium current that produces a negative-going action potential in Ascaris muscle.", "content": "1. A voltage clamp has been developed for the pharyngeal muscle of the nematode Ascaris lumbricoides and has been used to analyse the potassium current that produces a negative-going, regenerative action potential in this muscle. 2. Depolarizing voltage steps elicit a sustained inward current; returning the membrane voltage to the resting level evokes a strong, transient, outward current. This outward current reverses direction at the same voltage as that reached by the negative-going spike and is identified as the negative spike current. 3. The negative spike current decays with a time constant of 30 msec at voltages more negative than -30mV. This inactivation of the negative spike conductance is removed by holding the membrane at potentials more positive than -15mV. The time constant for removal of inactivation decreases from more than 300 msec at -15 mV to about 30 msec at +10 mV. 4. When inactivation has been removed, the negative spike conductance is turned on by stepping to potentials more negative than -15 mV. 5. Although the reversal potential for this current depends strongly on [K+]o (42 mV/decade), the potential at which the conductance is turned on is independent of [K+]o. 6. External Na+ seems to facilitate the negative spike current. Reduction of [Na+]o reduces its conductance and shifts the reversal potential to more positive values. 7. External Rb+ and Cs+ show voltage-dependent blocking of this current. 8. This K current is different from all the K currents which have been studied previously; however, it is analogous to the classical Na current of nerve and muscle, except for an inversion of the voltage dependencies.", "contents": "Voltage-clamp analysis of the potassium current that produces a negative-going action potential in Ascaris muscle. 1. A voltage clamp has been developed for the pharyngeal muscle of the nematode Ascaris lumbricoides and has been used to analyse the potassium current that produces a negative-going, regenerative action potential in this muscle. 2. Depolarizing voltage steps elicit a sustained inward current; returning the membrane voltage to the resting level evokes a strong, transient, outward current. This outward current reverses direction at the same voltage as that reached by the negative-going spike and is identified as the negative spike current. 3. The negative spike current decays with a time constant of 30 msec at voltages more negative than -30mV. This inactivation of the negative spike conductance is removed by holding the membrane at potentials more positive than -15mV. The time constant for removal of inactivation decreases from more than 300 msec at -15 mV to about 30 msec at +10 mV. 4. When inactivation has been removed, the negative spike conductance is turned on by stepping to potentials more negative than -15 mV. 5. Although the reversal potential for this current depends strongly on [K+]o (42 mV/decade), the potential at which the conductance is turned on is independent of [K+]o. 6. External Na+ seems to facilitate the negative spike current. Reduction of [Na+]o reduces its conductance and shifts the reversal potential to more positive values. 7. External Rb+ and Cs+ show voltage-dependent blocking of this current. 8. This K current is different from all the K currents which have been studied previously; however, it is analogous to the classical Na current of nerve and muscle, except for an inversion of the voltage dependencies."}
{"id": "PMID:469719", "title": "Extracellular space and diffusion barriers in muscle fibres from Megabalanus psittacus (Darwin).", "content": "1. Muscle fibres from Megabalanus psittacus (Darwin) were used to measure the exchange of Na+ and Ca2+ between intracellular and extracellular compartments. 2. The size of the extracellular space was evaluated directly from electron micrographs at 6.1 +/- 0.5% (percentage of the fibre volume). The more conventional estimates using Na+ and 134Cs+ as space markers gave higher values, namely, 8.9 +/- 0.7 and 9.5 +/- 1.5%. 3. An average value of 9.2% was used to correct the total Na+ and K+ in the muscle fibres and to estimate the intracellular concentrations of Na+ and K+ as 39 +/- 4 and 202 +/- 11 mM respectively. 4. Na+ (and similar Ca2+) washout curves could be described using a three compartments diffusion model. The data analysed in terms of this model enabled us to estimate membrane permeabilities as PNa+ = 2.7 x 10(-7) and PCa2+ = 2.7 x 10(-7) cm/sec.", "contents": "Extracellular space and diffusion barriers in muscle fibres from Megabalanus psittacus (Darwin). 1. Muscle fibres from Megabalanus psittacus (Darwin) were used to measure the exchange of Na+ and Ca2+ between intracellular and extracellular compartments. 2. The size of the extracellular space was evaluated directly from electron micrographs at 6.1 +/- 0.5% (percentage of the fibre volume). The more conventional estimates using Na+ and 134Cs+ as space markers gave higher values, namely, 8.9 +/- 0.7 and 9.5 +/- 1.5%. 3. An average value of 9.2% was used to correct the total Na+ and K+ in the muscle fibres and to estimate the intracellular concentrations of Na+ and K+ as 39 +/- 4 and 202 +/- 11 mM respectively. 4. Na+ (and similar Ca2+) washout curves could be described using a three compartments diffusion model. The data analysed in terms of this model enabled us to estimate membrane permeabilities as PNa+ = 2.7 x 10(-7) and PCa2+ = 2.7 x 10(-7) cm/sec."}
{"id": "PMID:469720", "title": "On the role of extracellular calcium in triggering contraction in muscle fibres from barnacle under membrane potential control.", "content": "1. Single giant barnacle muscle fibres from Megabalanus psittacus (Darwin) were used to establish the possible role of extracellular Ca2+ in triggering the contraction. 2. Peak tension p0 during twitches elicited by depolarizing voltage clamp pulses decreases after the removal of Ca2+ from the external saline. p0 becomes too small to measure after a few minutes, when the model-estimated level of total intracellular Ca has been reduced to 99.5% of its original value and the extracellular Ca in the clefts and/or tubules to about 10%. 3. In fibres where the outward currents were blocked by internal perfusion with Cs+, the curves of the integral of the inward current versus membrane potential and of peak tension versus membrane potential follow a similar pattern. 4. When Ca2+ in the external saline was replaced by Ba2+ or Sr2+, no twitches were generated although depolarizing voltage clamp pulses induced large inward currents comparable to those measured when Ca2+ was present. 5. It is concluded that an increase in Ca2+ in the fibre during the Ca2+ inward current is a necessary condition for the triggering of the development of tension. The data on Ba2+ inhibition is consistent with the hypothesis that there are two different intracellular sites of action for Ca2+.", "contents": "On the role of extracellular calcium in triggering contraction in muscle fibres from barnacle under membrane potential control. 1. Single giant barnacle muscle fibres from Megabalanus psittacus (Darwin) were used to establish the possible role of extracellular Ca2+ in triggering the contraction. 2. Peak tension p0 during twitches elicited by depolarizing voltage clamp pulses decreases after the removal of Ca2+ from the external saline. p0 becomes too small to measure after a few minutes, when the model-estimated level of total intracellular Ca has been reduced to 99.5% of its original value and the extracellular Ca in the clefts and/or tubules to about 10%. 3. In fibres where the outward currents were blocked by internal perfusion with Cs+, the curves of the integral of the inward current versus membrane potential and of peak tension versus membrane potential follow a similar pattern. 4. When Ca2+ in the external saline was replaced by Ba2+ or Sr2+, no twitches were generated although depolarizing voltage clamp pulses induced large inward currents comparable to those measured when Ca2+ was present. 5. It is concluded that an increase in Ca2+ in the fibre during the Ca2+ inward current is a necessary condition for the triggering of the development of tension. The data on Ba2+ inhibition is consistent with the hypothesis that there are two different intracellular sites of action for Ca2+."}
{"id": "PMID:469721", "title": "Changes in mammary function at the onset of lactation in the goat: correlation with hormonal changes.", "content": "1. Changes in mammary function and plasma hormone concentrations during late pregnancy and the onset of lactation have been studied in conscious goats.2. Mammary blood flow, oxygen consumption and glucose uptake increased markedly and significantly between 2 days and 0.5-1 day pre-partum.3. The increase in mammary glucose uptake was relatively greater than that of oxygen consumption or blood flow.4. The concentration of citrate in mammary secretion increased; the first significant change was apparent 0.5-1 day pre-partum but the main rise occurred after this time.5. It is concluded that the marked increase in mammary glucose uptake 0.5-1 day before parturition indicates the time of onset of copious milk secretion.6. The first significant increase in the concentration of unconjugated oestrogens in arterial plasma occurred 3 days pre-partum, whereas the first significant decrease in progesterone and increase in 13,14-dihydro-15-oxoprostaglandin F(2alpha) occurred 0.5-1 day pre-partum, thus coinciding with the mammary changes; there was also a peak in prolactin concentrations at the latter time.7. The hormonal changes are discussed in relation to current concepts of the initiation of parturition and the onset of copious milk secretion in this species. It is suggested that the fall in plasma progesterone concentrations triggers milk secretion at high rates.", "contents": "Changes in mammary function at the onset of lactation in the goat: correlation with hormonal changes. 1. Changes in mammary function and plasma hormone concentrations during late pregnancy and the onset of lactation have been studied in conscious goats.2. Mammary blood flow, oxygen consumption and glucose uptake increased markedly and significantly between 2 days and 0.5-1 day pre-partum.3. The increase in mammary glucose uptake was relatively greater than that of oxygen consumption or blood flow.4. The concentration of citrate in mammary secretion increased; the first significant change was apparent 0.5-1 day pre-partum but the main rise occurred after this time.5. It is concluded that the marked increase in mammary glucose uptake 0.5-1 day before parturition indicates the time of onset of copious milk secretion.6. The first significant increase in the concentration of unconjugated oestrogens in arterial plasma occurred 3 days pre-partum, whereas the first significant decrease in progesterone and increase in 13,14-dihydro-15-oxoprostaglandin F(2alpha) occurred 0.5-1 day pre-partum, thus coinciding with the mammary changes; there was also a peak in prolactin concentrations at the latter time.7. The hormonal changes are discussed in relation to current concepts of the initiation of parturition and the onset of copious milk secretion in this species. It is suggested that the fall in plasma progesterone concentrations triggers milk secretion at high rates."}
{"id": "PMID:469722", "title": "Free-flow reabsorption of glucose, sodium, osmoles and water in rat proximal convoluted tubule.", "content": "1. Reabsorption of glucose, sodium, total solute (osmoles) and water in the rat proximal tubule (pars convoluta) were studied by free-flow micropuncture at normal (saline-infused), suppressed (saline with phlorizin) and elevated (glucose infusion) glucose reabsorption rates. 2. Phlorizin completely inhibited net glucose reabsorption, approximately halved reabsorption of sodium, total solutes and water, and reduced single nephron glomerular filtration rate (SNGFR). 3. In saline and glucose-infused groups, there were no significant differences between SNGFR nor between reabsorptions (fractional and absolute) of either sodium, total solute or water, which were uniformly distributed along segments assessible to micropuncture. 4. Glucose reabsorptive capacity existed along the entire pars convoluta, with highest reabsorptive rates in convolutions closest to the glomerulus (in saline-infused rats, 90% fractional reabsorption at 2 mm, over 95% at end pars convoluta; in glucose-infused rats, 55 and 90%, respectively). 5. In saline and glucose infused rats, a significant correlation existed between net glucose and sodium reabsorption, but the regression slopes differed and correlations became non-significant when the reabsorptive fluxes were factored by SNGFR. 6. For all groups, the majority of tubular fluid (TF) concentrations of osmoles and sodium were lower than those in plasma (over-all mean TFosm)Posm = 0.973 +/- 0.004, P less than 0.001; TFNa /PNa = 0.964 +/- 0.005, P less than 0.001). 7. Correspondingly, calculated osmolal and sodium concentrations in the reabsorbate were greater than those in plasma, and were significantly correlated with distance to puncture site with maximal values in the most proximal convolutions (for osmolality, approximately +79 m-osmole kg-1 water at 1 mm).", "contents": "Free-flow reabsorption of glucose, sodium, osmoles and water in rat proximal convoluted tubule. 1. Reabsorption of glucose, sodium, total solute (osmoles) and water in the rat proximal tubule (pars convoluta) were studied by free-flow micropuncture at normal (saline-infused), suppressed (saline with phlorizin) and elevated (glucose infusion) glucose reabsorption rates. 2. Phlorizin completely inhibited net glucose reabsorption, approximately halved reabsorption of sodium, total solutes and water, and reduced single nephron glomerular filtration rate (SNGFR). 3. In saline and glucose-infused groups, there were no significant differences between SNGFR nor between reabsorptions (fractional and absolute) of either sodium, total solute or water, which were uniformly distributed along segments assessible to micropuncture. 4. Glucose reabsorptive capacity existed along the entire pars convoluta, with highest reabsorptive rates in convolutions closest to the glomerulus (in saline-infused rats, 90% fractional reabsorption at 2 mm, over 95% at end pars convoluta; in glucose-infused rats, 55 and 90%, respectively). 5. In saline and glucose infused rats, a significant correlation existed between net glucose and sodium reabsorption, but the regression slopes differed and correlations became non-significant when the reabsorptive fluxes were factored by SNGFR. 6. For all groups, the majority of tubular fluid (TF) concentrations of osmoles and sodium were lower than those in plasma (over-all mean TFosm)Posm = 0.973 +/- 0.004, P less than 0.001; TFNa /PNa = 0.964 +/- 0.005, P less than 0.001). 7. Correspondingly, calculated osmolal and sodium concentrations in the reabsorbate were greater than those in plasma, and were significantly correlated with distance to puncture site with maximal values in the most proximal convolutions (for osmolality, approximately +79 m-osmole kg-1 water at 1 mm)."}
{"id": "PMID:469723", "title": "The effect of bupivacaine aerosol on the activity of pulmonary stretch and 'irritant' receptors.", "content": "1. Experiments have been done on anaesthetized, paralysed and artificially ventilated cats. 2. Vagal single afferent fibres showing discharges in phase with respiration were isolated in the neck. Two types of fibres were studied at different tidal volumes: (i) showing slowly adapting discharges from the pulmonary stretch receptors; (ii) showing rapidly adapting discharges from the lung 'irritant' receptors. The effect of bupivacaine aerosol, administered by positive pressure inflations, was recorded on the pattern of fibre discharge. 3. The pulmonary stretch fibres were further classified into low-threshold and higher-threshold fibres according to the standard criteria. Bupivacaine aerosol blocked activity in all the low-threshold and in the majority of the higher-threshold fibres. 4. Of the rapidly adapting fibres, bupivacaine completely blocked activity at some tidal volumes and markedly reduced it at most others. 5. The fibre activity data are presented. It is concluded that although bupivacaine aerosol markedly reduced impulse activity in all three types of fibres, the data suggest that there is a small difference in the ease with which low-threshold fibres on the one hand and higher-threshold and \"irritant\" fibres on the other hand are affected. The reasons for this difference in the behaviour are not understood.", "contents": "The effect of bupivacaine aerosol on the activity of pulmonary stretch and 'irritant' receptors. 1. Experiments have been done on anaesthetized, paralysed and artificially ventilated cats. 2. Vagal single afferent fibres showing discharges in phase with respiration were isolated in the neck. Two types of fibres were studied at different tidal volumes: (i) showing slowly adapting discharges from the pulmonary stretch receptors; (ii) showing rapidly adapting discharges from the lung 'irritant' receptors. The effect of bupivacaine aerosol, administered by positive pressure inflations, was recorded on the pattern of fibre discharge. 3. The pulmonary stretch fibres were further classified into low-threshold and higher-threshold fibres according to the standard criteria. Bupivacaine aerosol blocked activity in all the low-threshold and in the majority of the higher-threshold fibres. 4. Of the rapidly adapting fibres, bupivacaine completely blocked activity at some tidal volumes and markedly reduced it at most others. 5. The fibre activity data are presented. It is concluded that although bupivacaine aerosol markedly reduced impulse activity in all three types of fibres, the data suggest that there is a small difference in the ease with which low-threshold fibres on the one hand and higher-threshold and \"irritant\" fibres on the other hand are affected. The reasons for this difference in the behaviour are not understood."}
{"id": "PMID:469724", "title": "Prostaglandins and the sources of their production in a mild inflammatory lesion in sheep.", "content": "1. Wound fluid was collected from a subcutaneous lesion in the shoulder region of sheep by implanting a PTFE sampling chamber from which a catheter led to the skin surface. 2. The neutrophil granulocyte numbers and prostaglandin content of the wound fluid were studied, prostaglandins being identified by  thin layer chromatography and measured by bio-assay. The major prostaglandin component was E2. 3. There was no clear relation between the amounts of prostaglandin present and the numbers of neutrophils in the fluid. 4. A subcutaneously implanted double chamber permitted perfusion of the lesion and thereby the study of the rate of prostaglandin production and the rate of disappearance of isotopically labelled prostaglandin from the lesion. The rate of leucocyte entry into the wound fluid could also be measured. 5. When the cutaneous muscle through which the chamber had been implanted was biopsied and incubated in vitro it was found to produce prostaglandin E2 in quantities which could be correlated with the weight of the incubated muscle. 6. These results suggest that a major contributor to the prostaglandin content of the wound fluid is the cutaneous striped muscle.", "contents": "Prostaglandins and the sources of their production in a mild inflammatory lesion in sheep. 1. Wound fluid was collected from a subcutaneous lesion in the shoulder region of sheep by implanting a PTFE sampling chamber from which a catheter led to the skin surface. 2. The neutrophil granulocyte numbers and prostaglandin content of the wound fluid were studied, prostaglandins being identified by  thin layer chromatography and measured by bio-assay. The major prostaglandin component was E2. 3. There was no clear relation between the amounts of prostaglandin present and the numbers of neutrophils in the fluid. 4. A subcutaneously implanted double chamber permitted perfusion of the lesion and thereby the study of the rate of prostaglandin production and the rate of disappearance of isotopically labelled prostaglandin from the lesion. The rate of leucocyte entry into the wound fluid could also be measured. 5. When the cutaneous muscle through which the chamber had been implanted was biopsied and incubated in vitro it was found to produce prostaglandin E2 in quantities which could be correlated with the weight of the incubated muscle. 6. These results suggest that a major contributor to the prostaglandin content of the wound fluid is the cutaneous striped muscle."}
{"id": "PMID:469725", "title": "The effect of dopamine on shivering in the rat.", "content": "1. Dopamine (0.5--0.2  mumole) injected into a lateral cerebral ventricle inhibited shivering and lowered core temperature in rats at an ambient temperature of 0--5 degrees C. 2. These effects were inhibited by the dopamine antagonist, pimozide, and imitated by the dopamine agonists, piribedil and apomorphine. 3. Pimozide itself had no effect on shivering. 4. It is concluded that while there are inhibitory dopamine receptors on the cold sensor--shivering pathway in the rat they are not concerned in the normal function of this pathway.", "contents": "The effect of dopamine on shivering in the rat. 1. Dopamine (0.5--0.2  mumole) injected into a lateral cerebral ventricle inhibited shivering and lowered core temperature in rats at an ambient temperature of 0--5 degrees C. 2. These effects were inhibited by the dopamine antagonist, pimozide, and imitated by the dopamine agonists, piribedil and apomorphine. 3. Pimozide itself had no effect on shivering. 4. It is concluded that while there are inhibitory dopamine receptors on the cold sensor--shivering pathway in the rat they are not concerned in the normal function of this pathway."}
{"id": "PMID:469726", "title": "Sural nerve effects on medial gastrocnemius motoneurones in the cat.", "content": "1. Excitability cycles for medial gastrocnemius motoneurones were recorded following sural nerve stimuli with amplitudes of 1.5, 3, 5, 10 and 40 times the nerve threshold. The monosynaptic reflex was time-integrated to quantify the motoneuronal output and some implications of this technique are discussed. In particular it is calculated that this method specifically examines the 15-25% most excitable motoneurones of the medial gastrocnemius pool.2. In most cases high amplitude stimulation of the sural nerve caused a triphasic (excitatory-inhibitory-excitatory) change in excitability. Arguments are given to support the conclusion that this represents a corresponding post-synaptic alteration in medial gastrocnemius motoneurone potential. It is concluded that this pattern illustrates the nature of the sural nerve projections to this portion of the medial gastrocnemius pool.3. The first period of excitation began after a latency, corrected to be compatible with intracellular recordings, of 2.8 ms and had a mean duration of 4.0 ms. The minimum stimulus level necessary for this effect lay between 1.5 and 3 times the nerve threshold. The maximum amplitude of this facilitation occurred with stimuli of 3-5 times threshold and was 60-90% of the amplitude of the monosynaptic facilitation which followed stimulation of the lateral gastrocnemius-soleus nerve.4. A period of inhibition followed immediately after the period of excitation and had a mean duration of 16 ms. The minimum stimulus level necessary for this effect lay between 1.5 and 3 times the nerve threshold and the degree of inhibition increased with stimuli up to 40 times threshold.5. The second period of excitation lasted a mean period of 50 ms and was due to activity in high threshold fibres. On average its amplitude was 50% that of the initial excitation.6. Exceptions to this triphasic variation of excitability were found. This result is interpreted as indicating the presence of projections with opposing actions which were simultaneously activated by sural stimuli.", "contents": "Sural nerve effects on medial gastrocnemius motoneurones in the cat. 1. Excitability cycles for medial gastrocnemius motoneurones were recorded following sural nerve stimuli with amplitudes of 1.5, 3, 5, 10 and 40 times the nerve threshold. The monosynaptic reflex was time-integrated to quantify the motoneuronal output and some implications of this technique are discussed. In particular it is calculated that this method specifically examines the 15-25% most excitable motoneurones of the medial gastrocnemius pool.2. In most cases high amplitude stimulation of the sural nerve caused a triphasic (excitatory-inhibitory-excitatory) change in excitability. Arguments are given to support the conclusion that this represents a corresponding post-synaptic alteration in medial gastrocnemius motoneurone potential. It is concluded that this pattern illustrates the nature of the sural nerve projections to this portion of the medial gastrocnemius pool.3. The first period of excitation began after a latency, corrected to be compatible with intracellular recordings, of 2.8 ms and had a mean duration of 4.0 ms. The minimum stimulus level necessary for this effect lay between 1.5 and 3 times the nerve threshold. The maximum amplitude of this facilitation occurred with stimuli of 3-5 times threshold and was 60-90% of the amplitude of the monosynaptic facilitation which followed stimulation of the lateral gastrocnemius-soleus nerve.4. A period of inhibition followed immediately after the period of excitation and had a mean duration of 16 ms. The minimum stimulus level necessary for this effect lay between 1.5 and 3 times the nerve threshold and the degree of inhibition increased with stimuli up to 40 times threshold.5. The second period of excitation lasted a mean period of 50 ms and was due to activity in high threshold fibres. On average its amplitude was 50% that of the initial excitation.6. Exceptions to this triphasic variation of excitability were found. This result is interpreted as indicating the presence of projections with opposing actions which were simultaneously activated by sural stimuli."}
{"id": "PMID:469728", "title": "Evidence for primary afferent depolarization of single tooth-pulp afferents in the cat.", "content": "1. Responses of single tooth-pulp afferents to electrical stimulation of sites in the trigeminal sensory nuclear complex were recorded from caine teeth in cats. 2. Changes in the excitability of the central terminals of a pulpal afferent after stimulation of other groups of sensory nerves were taken as evidence for changes in their polarization. 3. Electrical stimulation of nerves in other teeth resulted in raised excitability of the central terminals of pulpal afferents lasting up to 300 msec. The greatest effects were observed 30--100 msec after the conditioning stimulus. 4. Increased terminal excitability was also observed after the ipsilateral infraorbital nerve was stimulated. This occurred when only nerve fibres with conduction velocities in the A alpha range were excited. 5. Mechanical stimulation of a canine tooth produced increases in terminal excitability of pulpal afferents innervating the same tooth. 6. A similar effect was also observed after a brief pull on a group of ipsilateral mystacial vibrissae. 7. No evidence of decreases in the excitability of the central terminals of tooth-pulp afferents was obtained with any of the conditioning stimuli.", "contents": "Evidence for primary afferent depolarization of single tooth-pulp afferents in the cat. 1. Responses of single tooth-pulp afferents to electrical stimulation of sites in the trigeminal sensory nuclear complex were recorded from caine teeth in cats. 2. Changes in the excitability of the central terminals of a pulpal afferent after stimulation of other groups of sensory nerves were taken as evidence for changes in their polarization. 3. Electrical stimulation of nerves in other teeth resulted in raised excitability of the central terminals of pulpal afferents lasting up to 300 msec. The greatest effects were observed 30--100 msec after the conditioning stimulus. 4. Increased terminal excitability was also observed after the ipsilateral infraorbital nerve was stimulated. This occurred when only nerve fibres with conduction velocities in the A alpha range were excited. 5. Mechanical stimulation of a canine tooth produced increases in terminal excitability of pulpal afferents innervating the same tooth. 6. A similar effect was also observed after a brief pull on a group of ipsilateral mystacial vibrissae. 7. No evidence of decreases in the excitability of the central terminals of tooth-pulp afferents was obtained with any of the conditioning stimuli."}
{"id": "PMID:469727", "title": "The effects of acetylcholine and dopamine on carotid chemosensory activity in the rabbit.", "content": "1. Intracarotid (I.C.) injection of either acetylcholine (ACh) or dopamine inhibited spontaneous chemosensory activity recorded from the peripheral cut end of the sinus nerve in the anaesthetized rabbit. 2. High doses of ACh (greater than or equal to 50 micrograms I.C.) evoked a slight increase in discharge which preceded the inhibition. This excitation was attributable to a nicotinic action of the drug since it was abolished by mecamylamine. 3. The muscarinic agonist bethanechol inhibited chemoreceptor activity, an effect which was blocked by high doses of atropine, as was the inhibition caused by ACh. Dopamine-induced inhibition was unaffected by atropine. 4. Atropine, in doses sufficient to abolish the vasodepressor effect of ACh, only slightly reduced the inhibitory action of ACh on the chemoreceptors. Also, the vasodilators sodium nitrite and sodium nitroprusside did not appreciably alter chemosensory discharge. It seems unlikely, therefore, that the inhibitory response to ACh is secondary to vascular changes. 5. The inhibitory response to dopamine, but not that to ACh, was blocked by the dopamine antagonist alpha-flupenthixol. This implies that inhibition of chemosensory activity evoked by exogenous ACh was not secondary to dopamine release. 6. The implications of the results are discussed, particularly with regard to the possible physiological role of ACh as a modulator of carotid chemosensory activity.", "contents": "The effects of acetylcholine and dopamine on carotid chemosensory activity in the rabbit. 1. Intracarotid (I.C.) injection of either acetylcholine (ACh) or dopamine inhibited spontaneous chemosensory activity recorded from the peripheral cut end of the sinus nerve in the anaesthetized rabbit. 2. High doses of ACh (greater than or equal to 50 micrograms I.C.) evoked a slight increase in discharge which preceded the inhibition. This excitation was attributable to a nicotinic action of the drug since it was abolished by mecamylamine. 3. The muscarinic agonist bethanechol inhibited chemoreceptor activity, an effect which was blocked by high doses of atropine, as was the inhibition caused by ACh. Dopamine-induced inhibition was unaffected by atropine. 4. Atropine, in doses sufficient to abolish the vasodepressor effect of ACh, only slightly reduced the inhibitory action of ACh on the chemoreceptors. Also, the vasodilators sodium nitrite and sodium nitroprusside did not appreciably alter chemosensory discharge. It seems unlikely, therefore, that the inhibitory response to ACh is secondary to vascular changes. 5. The inhibitory response to dopamine, but not that to ACh, was blocked by the dopamine antagonist alpha-flupenthixol. This implies that inhibition of chemosensory activity evoked by exogenous ACh was not secondary to dopamine release. 6. The implications of the results are discussed, particularly with regard to the possible physiological role of ACh as a modulator of carotid chemosensory activity."}
{"id": "PMID:469729", "title": "Sodium transport in the hen lower intestine. induction of sodium sites in the brush border by a low sodium diet.", "content": "1. The fluxes of Na were measured on isolated coprodeal mucosa at 1--220 mM-Na from hens on low (L) and high (H) Na diets with the purpose of finding the location and characteristics of Na sites activated in the cellular pathway by L. 2. The influx across the brush border, JNamc, and the transmural fluxes, JNasm and JNams, were determined. Effects on these fluxes of ouabain, 10(-3) M in the serosal solution, and amiloride, 10(-4) M in the mucosal solution, were studied for both dietary states. 3. JNamc was 5--22 (L) and 0--0.8 (H) muequiv/cm2.hr at 130 mM-Na corrected for the paracellular flux of Na. The JNamc (H) is tenfold smaller than found by Choshniak, Munck & Skadhauge (1977). This discrepancy is at present inexplicable. Amiloride completely inhibited JNamc (L). Preincubation in 0 or 130 mM-Na had no effect on JNamc. Ouabain reduced JNamc (L) by only about 37% after preincubation at 130 mM-Na. The Kt of JNamc was 5.1 (L) and 50.6 (H) mM-Na. 4. JNasm was 50 (H) and 61 (L) n-equiv/cm2.hr at 6.5 mM-Na. Ouabain increased JNasm by 360% in the low Na state. The increased JNasm was inhibited 74--100% by amiloride. This is interpreted as a ouabain induced Na-Na exchange at the basolateral Na-K-ATPase and an almost complete block of JNacm by amiloride. A similar exchange of Na at the basolateral membrane in the high-Na state was revealed by 'opening' the brush border for Na with monensin added to the mucosal solution. Amiloride in itself prevented a 50% recirculation of Na via the paracellular route and back across the cells in the low Na state. 5. JNams was 5.6 (L) muequiv/cm2.hr and 187 (L) microA/cm2 at 6.5 mM-Na. Amiloride reduced these values to 0.4 muequiv/cm2.hr and 5.8 microA/cm2. On addition of amiloride the transmural resistance in (L) coprodea at 130 mM-Na increased from 140 to 190 and it remained unchanged at 260 omega cm2 in (H) coprodea. The resistance of (L) birds, 163, was not affected by ouabain, 166 (L) omega cm2. 6. 20:1 NaCl dilution potentials at the mucosal side of 17--18 mV (L) and nearly zero (H) had half-times around 1 sec. Amiloride eliminated completely these diffusion potentials. The short half-time indicates a location in the brush border of sodium specific sites induced by the low-Na diet. This conclusion is oppsite to that described by Choshniak et al. (1977). 7. Ion selectivity, voltage--current and conductance--concentration relations in the presence of amiloride indicated a weakly cation selective and highly hydrated pathway, which was also thick and with neutral sites. This fits a paracellular route with the limiting barrier for ions at the tight junction.", "contents": "Sodium transport in the hen lower intestine. induction of sodium sites in the brush border by a low sodium diet. 1. The fluxes of Na were measured on isolated coprodeal mucosa at 1--220 mM-Na from hens on low (L) and high (H) Na diets with the purpose of finding the location and characteristics of Na sites activated in the cellular pathway by L. 2. The influx across the brush border, JNamc, and the transmural fluxes, JNasm and JNams, were determined. Effects on these fluxes of ouabain, 10(-3) M in the serosal solution, and amiloride, 10(-4) M in the mucosal solution, were studied for both dietary states. 3. JNamc was 5--22 (L) and 0--0.8 (H) muequiv/cm2.hr at 130 mM-Na corrected for the paracellular flux of Na. The JNamc (H) is tenfold smaller than found by Choshniak, Munck & Skadhauge (1977). This discrepancy is at present inexplicable. Amiloride completely inhibited JNamc (L). Preincubation in 0 or 130 mM-Na had no effect on JNamc. Ouabain reduced JNamc (L) by only about 37% after preincubation at 130 mM-Na. The Kt of JNamc was 5.1 (L) and 50.6 (H) mM-Na. 4. JNasm was 50 (H) and 61 (L) n-equiv/cm2.hr at 6.5 mM-Na. Ouabain increased JNasm by 360% in the low Na state. The increased JNasm was inhibited 74--100% by amiloride. This is interpreted as a ouabain induced Na-Na exchange at the basolateral Na-K-ATPase and an almost complete block of JNacm by amiloride. A similar exchange of Na at the basolateral membrane in the high-Na state was revealed by 'opening' the brush border for Na with monensin added to the mucosal solution. Amiloride in itself prevented a 50% recirculation of Na via the paracellular route and back across the cells in the low Na state. 5. JNams was 5.6 (L) muequiv/cm2.hr and 187 (L) microA/cm2 at 6.5 mM-Na. Amiloride reduced these values to 0.4 muequiv/cm2.hr and 5.8 microA/cm2. On addition of amiloride the transmural resistance in (L) coprodea at 130 mM-Na increased from 140 to 190 and it remained unchanged at 260 omega cm2 in (H) coprodea. The resistance of (L) birds, 163, was not affected by ouabain, 166 (L) omega cm2. 6. 20:1 NaCl dilution potentials at the mucosal side of 17--18 mV (L) and nearly zero (H) had half-times around 1 sec. Amiloride eliminated completely these diffusion potentials. The short half-time indicates a location in the brush border of sodium specific sites induced by the low-Na diet. This conclusion is oppsite to that described by Choshniak et al. (1977). 7. Ion selectivity, voltage--current and conductance--concentration relations in the presence of amiloride indicated a weakly cation selective and highly hydrated pathway, which was also thick and with neutral sites. This fits a paracellular route with the limiting barrier for ions at the tight junction."}
{"id": "PMID:469730", "title": "The action of angiotensin II on the baroreflex response of the conscious ewe and the conscious fetus.", "content": "1. In conscious non-pregnant and pregnant ewes and in chronic fetal lamb preparations, the beat by beat relationship between pulse interval and systolic pressure was studied during acute elevations in arterial pressure induced by phenylephrine. Baroreflex sensitivity, which was defined as the slope of the pressure-pulse interval relationship when phenylephrine was used to raise pressure, was abolished by atropine and increased by propranolol. Baroreflex sensitivity was less in pregnant ewes and in foetal lambs compared with non-pregnant ewes. 2. These findings suggest that the vagus nerve is responsible for the reflex bradycardia that occurs in the foetus and the ewe when arterial pressure is increased. 3. In both fetal and adult sheep, actue hypertension due to I.V. injection of angiotensin II was not associated with a consistent and progressive bradycardia, such as was seen with acute hypertension caused by phenylephrine. Angiotensin II has no direct chronotropic effect on heart rate in either the adult or the fetus. 4. No linear relationship between arterial pressure and pulse interval was seen when angiotensin II was used to raise pressure in sheep which were treated with propranolol. Therefore the lack of cardiac slowing with pressor doses of angiotensin II was not due to concomitant activation of the sympathoadrenal system. 5. It is concluded that in both fetal and adult sheep angiotensin II reduces the increase in vagal tone which is responsible for slowing of heart rate in response to acute rises in arterial pressure.", "contents": "The action of angiotensin II on the baroreflex response of the conscious ewe and the conscious fetus. 1. In conscious non-pregnant and pregnant ewes and in chronic fetal lamb preparations, the beat by beat relationship between pulse interval and systolic pressure was studied during acute elevations in arterial pressure induced by phenylephrine. Baroreflex sensitivity, which was defined as the slope of the pressure-pulse interval relationship when phenylephrine was used to raise pressure, was abolished by atropine and increased by propranolol. Baroreflex sensitivity was less in pregnant ewes and in foetal lambs compared with non-pregnant ewes. 2. These findings suggest that the vagus nerve is responsible for the reflex bradycardia that occurs in the foetus and the ewe when arterial pressure is increased. 3. In both fetal and adult sheep, actue hypertension due to I.V. injection of angiotensin II was not associated with a consistent and progressive bradycardia, such as was seen with acute hypertension caused by phenylephrine. Angiotensin II has no direct chronotropic effect on heart rate in either the adult or the fetus. 4. No linear relationship between arterial pressure and pulse interval was seen when angiotensin II was used to raise pressure in sheep which were treated with propranolol. Therefore the lack of cardiac slowing with pressor doses of angiotensin II was not due to concomitant activation of the sympathoadrenal system. 5. It is concluded that in both fetal and adult sheep angiotensin II reduces the increase in vagal tone which is responsible for slowing of heart rate in response to acute rises in arterial pressure."}
{"id": "PMID:469731", "title": "Actions of a carbocyanine dye on calcium-dependent potassium transport in human red cell ghosts.", "content": "1. 3,3'-Diethylthiadicarbocyanine iodide (diS-C2-(5)), a fluorescent dye widely used to estimate membrane potentials, inhibits the Ca-dependent K transport system of human red cells and ghosts. It does not affect non-specific cation leaks, net chloride movements or the Ca-activated ATPase. 2. The nature of the inhibitory effect depends upon the conditions under which the dye is applied. When Ca-containing ghosts with 100 mM internal K are suspended in a choline medium at 37 degrees C, 0.3 microM-diS-C2-(5) causes virtually complete inhibition of K transport within 1 min (haematocrit 0.3%). Inhibition cannot be reversed by washing the ghosts to remove bound dye, although partial recovery occurs on subsequent incubation at 37 degrees C. 3. Inhibition is less marked when ghosts are suspended in solutions containing 100 mM-K, and is largely reversed by washing. The degree of inhibition varies with the dye concentration: 50% inhibition occurs with a free diS-C2-(5) concentration of 0.7 microM, and about 5 x 10(6) dye molecules are bound per ghost. 4. Changes in internal and external K concentrations, keeping internal Ca constant, have no effect on the dye concentration required for 50% inhibition of K efflux. 5. When internal Ca is varied, keeping internal and external K constant, the dye concentration needed for 50% inhibition of K efflux varies inversely with the K efflux in the absence of dye. That is, diS-C2-(5) is most effective as an inhibitor when K efflux is at a maximum. This suggests that the dye reacts preferentially with the transport system in the Ca-activated state. 6. The effects of several related molecules were examined, using Rb influx as a measure of K permeability. Inhibition is obtained in some cases, but is not directly related to the ability of the ghosts to bind the dyes. An anionic dye, diS-C3SO3--(5) inhibits Rb influx only when present inside the ghosts. 7. The implications of these findings are discussed.", "contents": "Actions of a carbocyanine dye on calcium-dependent potassium transport in human red cell ghosts. 1. 3,3'-Diethylthiadicarbocyanine iodide (diS-C2-(5)), a fluorescent dye widely used to estimate membrane potentials, inhibits the Ca-dependent K transport system of human red cells and ghosts. It does not affect non-specific cation leaks, net chloride movements or the Ca-activated ATPase. 2. The nature of the inhibitory effect depends upon the conditions under which the dye is applied. When Ca-containing ghosts with 100 mM internal K are suspended in a choline medium at 37 degrees C, 0.3 microM-diS-C2-(5) causes virtually complete inhibition of K transport within 1 min (haematocrit 0.3%). Inhibition cannot be reversed by washing the ghosts to remove bound dye, although partial recovery occurs on subsequent incubation at 37 degrees C. 3. Inhibition is less marked when ghosts are suspended in solutions containing 100 mM-K, and is largely reversed by washing. The degree of inhibition varies with the dye concentration: 50% inhibition occurs with a free diS-C2-(5) concentration of 0.7 microM, and about 5 x 10(6) dye molecules are bound per ghost. 4. Changes in internal and external K concentrations, keeping internal Ca constant, have no effect on the dye concentration required for 50% inhibition of K efflux. 5. When internal Ca is varied, keeping internal and external K constant, the dye concentration needed for 50% inhibition of K efflux varies inversely with the K efflux in the absence of dye. That is, diS-C2-(5) is most effective as an inhibitor when K efflux is at a maximum. This suggests that the dye reacts preferentially with the transport system in the Ca-activated state. 6. The effects of several related molecules were examined, using Rb influx as a measure of K permeability. Inhibition is obtained in some cases, but is not directly related to the ability of the ghosts to bind the dyes. An anionic dye, diS-C3SO3--(5) inhibits Rb influx only when present inside the ghosts. 7. The implications of these findings are discussed."}
{"id": "PMID:469732", "title": "The control of blood flow through human forearm muscles following brief isometric contractions.", "content": "1. The blood flow through the forearm was measured 2 sec after single, brief isometric hand-grip contractions. The tension and duration of those contractions varied from 10 to 100% of the maximal voluntary contraction (m.v.c.) and from 2 to 12 sec, respectively. 2. The blood flow increased linearly with tension up to about 60% m.v.c. but further increases in tension, up to 100% m.v.c., did not elicit higher blood flows than were found at 60% m.v.c. The same relationship between tension and the resultant blood flow held for all durations of contractions, from 2 to 12 sec. The blood flow immediately after (2 sec) contractions at a given tension increased linearly with the duration of the contraction, from 2 to 12 sec. Maximal exercise blood flow was approached only in response to the longest contractions (12 sec) at tensions of 60% m.v.c. or higher. 3. Brief alterations (2--5 sec) of transmural pressure across blood vessels did not result in a significant change of blood flow, either in the resting forearm or when the vessels were dilated by brief, isometric contractions. When the tension was applied or released either rapidly or gradually ('ramp' contractions) there was no correlation between the rate of change of stretch on arterial vessels and the resultant blood flow. However, there was a direct relationship between a force--time integral (duration of contraction x peak tension) and blood flow. All these results make it clear that changes in blood flow in the forearm elicited by brief isometric contractions are not the result of a myogenic reflex but are metabolically induced. 4. Successive contractions exerted at 60% m.v.c. for 4 sec induced a blood flow of 21.2 +/- 1.6 ml.min-1.100 ml.-1 when a rest interval of 8 sec was allowed between the contractions. Blood flows remained constant at this submaximal level, even when muscular fatigue was induced, and when there was an accompanying large increase in blood pressure. 5. Isometric muscular activity by the contralateral arm which resulted in fatigue, associated with a large increase in mean blood pressure, did not alter the level of vasodilation that was induced by brief, isometric contractions in the 'test' arm. 6. It is suggested that the vasodilatation in response to intermittent isometric contractions is the result of metabolic vasodilatation of distal segments and continued sympathetic vasoconstriction of the proximal segments of the forearm vascular bed.", "contents": "The control of blood flow through human forearm muscles following brief isometric contractions. 1. The blood flow through the forearm was measured 2 sec after single, brief isometric hand-grip contractions. The tension and duration of those contractions varied from 10 to 100% of the maximal voluntary contraction (m.v.c.) and from 2 to 12 sec, respectively. 2. The blood flow increased linearly with tension up to about 60% m.v.c. but further increases in tension, up to 100% m.v.c., did not elicit higher blood flows than were found at 60% m.v.c. The same relationship between tension and the resultant blood flow held for all durations of contractions, from 2 to 12 sec. The blood flow immediately after (2 sec) contractions at a given tension increased linearly with the duration of the contraction, from 2 to 12 sec. Maximal exercise blood flow was approached only in response to the longest contractions (12 sec) at tensions of 60% m.v.c. or higher. 3. Brief alterations (2--5 sec) of transmural pressure across blood vessels did not result in a significant change of blood flow, either in the resting forearm or when the vessels were dilated by brief, isometric contractions. When the tension was applied or released either rapidly or gradually ('ramp' contractions) there was no correlation between the rate of change of stretch on arterial vessels and the resultant blood flow. However, there was a direct relationship between a force--time integral (duration of contraction x peak tension) and blood flow. All these results make it clear that changes in blood flow in the forearm elicited by brief isometric contractions are not the result of a myogenic reflex but are metabolically induced. 4. Successive contractions exerted at 60% m.v.c. for 4 sec induced a blood flow of 21.2 +/- 1.6 ml.min-1.100 ml.-1 when a rest interval of 8 sec was allowed between the contractions. Blood flows remained constant at this submaximal level, even when muscular fatigue was induced, and when there was an accompanying large increase in blood pressure. 5. Isometric muscular activity by the contralateral arm which resulted in fatigue, associated with a large increase in mean blood pressure, did not alter the level of vasodilation that was induced by brief, isometric contractions in the 'test' arm. 6. It is suggested that the vasodilatation in response to intermittent isometric contractions is the result of metabolic vasodilatation of distal segments and continued sympathetic vasoconstriction of the proximal segments of the forearm vascular bed."}
{"id": "PMID:469733", "title": "The change in the load-sustaining ability and in the series elasticity in Mytilus smooth muscle during isotonic shortening.", "content": "1. The change in the ability to sustain a load and the change in the series elasticity in the anterior byssal retractor muscle (a.b.r.m.) of Mytilus edulis during isotonic shortening was studied by recording the length changes following step changes in load. 2. When a load of 0.7--0.9 P0 WAs applied after a period of isotonic shortening under a small load (0.05--0.1 P0), the muscle fibres showed continuous isotonic lengthening, indicating a reduction in the ability to sustain a load during isotonic shortening. 3. Following the application of a load of 0.3--0.6 P0 during isotonic shortening under a small load, the fibres exhibited a transient isotonic lengthening before starting to shorten isotonically, indicating some degree of restoration in the load-sustaining ability after the step increase in load. 4. No appreciable reduction in the load-sustaining ability was observed during isotonic shortening under a large load (more than 0.7 P0). 5. The load--extension curves of the series elasticity determined during isotonic shortening were found to be scaled down roughly in proportion to the isotonic load. 6. The stiffness of the muscle fibres during the isotonic shortening approached a certain finite value, when the isotonic load tended to zero. If the stiffness was measured during the development of isometric tension, the stiffness--isometric tension curve extrapolated towards the origin. 7. High-speed cinematography during the step change in load indicated a fairly uniform distribution of the series elasticity along the length of the preparation. 8. These results are discussed in relation to the sliding filament model of muscle contraction.", "contents": "The change in the load-sustaining ability and in the series elasticity in Mytilus smooth muscle during isotonic shortening. 1. The change in the ability to sustain a load and the change in the series elasticity in the anterior byssal retractor muscle (a.b.r.m.) of Mytilus edulis during isotonic shortening was studied by recording the length changes following step changes in load. 2. When a load of 0.7--0.9 P0 WAs applied after a period of isotonic shortening under a small load (0.05--0.1 P0), the muscle fibres showed continuous isotonic lengthening, indicating a reduction in the ability to sustain a load during isotonic shortening. 3. Following the application of a load of 0.3--0.6 P0 during isotonic shortening under a small load, the fibres exhibited a transient isotonic lengthening before starting to shorten isotonically, indicating some degree of restoration in the load-sustaining ability after the step increase in load. 4. No appreciable reduction in the load-sustaining ability was observed during isotonic shortening under a large load (more than 0.7 P0). 5. The load--extension curves of the series elasticity determined during isotonic shortening were found to be scaled down roughly in proportion to the isotonic load. 6. The stiffness of the muscle fibres during the isotonic shortening approached a certain finite value, when the isotonic load tended to zero. If the stiffness was measured during the development of isometric tension, the stiffness--isometric tension curve extrapolated towards the origin. 7. High-speed cinematography during the step change in load indicated a fairly uniform distribution of the series elasticity along the length of the preparation. 8. These results are discussed in relation to the sliding filament model of muscle contraction."}
{"id": "PMID:469734", "title": "The effects of tenotomy upon the contraction characteristics of motor units in rabbit soleus muscle.", "content": "1. Isometric contractions of tenotomized rabbit solei have been compared with a group of unoperated control muscles. A large decrease in the tension developed by the tenotomized muscle was accompanied by a slight shortening of the twitch contraction time, and a larger, progressive, reduction in the time to half relaxation.2. A comparison of motor units obtained from muscles which had been tenotomized for six weeks with those of the control muscles showed a large reduction in the range of contraction and half relaxation times of the units from the tenotomized muscles.3. The mean motor unit tension (expressed as a percentage of the whole muscle tension) was similar for both those units from the control muscles and those from muscles which had been tenotomized for six weeks, indicating a uniform atrophy of motor units within the tenotomized muscles.4. It was concluded that the change in the pattern of motor unit contraction times was not the result of a process of differential atrophy favouring the preservation of the faster contracting motor units.5. A correlation between axon conduction velocity and both the speed of contraction and the size (tension) of the motor units was demonstrated in the control muscles. Following tenotomy the relationship between axon conduction velocity and motor unit tension was lost.", "contents": "The effects of tenotomy upon the contraction characteristics of motor units in rabbit soleus muscle. 1. Isometric contractions of tenotomized rabbit solei have been compared with a group of unoperated control muscles. A large decrease in the tension developed by the tenotomized muscle was accompanied by a slight shortening of the twitch contraction time, and a larger, progressive, reduction in the time to half relaxation.2. A comparison of motor units obtained from muscles which had been tenotomized for six weeks with those of the control muscles showed a large reduction in the range of contraction and half relaxation times of the units from the tenotomized muscles.3. The mean motor unit tension (expressed as a percentage of the whole muscle tension) was similar for both those units from the control muscles and those from muscles which had been tenotomized for six weeks, indicating a uniform atrophy of motor units within the tenotomized muscles.4. It was concluded that the change in the pattern of motor unit contraction times was not the result of a process of differential atrophy favouring the preservation of the faster contracting motor units.5. A correlation between axon conduction velocity and both the speed of contraction and the size (tension) of the motor units was demonstrated in the control muscles. Following tenotomy the relationship between axon conduction velocity and motor unit tension was lost."}
{"id": "PMID:469739", "title": "Descending influences on the responses of spinocervical tract neurones to chemical stimulation of fine muscle afferents.", "content": "1. In cats, extracellular micro-electrode recordings were made from axons of the spinocervical tract (s.c.t.) in both the decerebrate state and during cold block of the spinal cord (reversible spinal state) to examine the effects of intra-arterial injection of algesic agents (bradykinin, potassium, 5-hydroxytryptamine) into the gastrocnemius-soleus (g.s.) muscle on the discharge behaviour of s.c.t. neurones.2. In the decerebrate state without cooling the spinal cord 13% of the cells (eleven out of eighty-three) responded to intra-arterial injection of bradykinin, 33% (twenty-two out of sixty-nine) to 5-hydroxytryptamine, and 38% (thirty-five out of ninety-one) to potassium injection.3. The general time course and the latency of the responses of s.c.t. cells induced by injection of pain-producing substances into the g.s. muscle reflect in many respects the activations of g.s. group III and group IV primary afferent units studied previously.4. For twenty-seven s.c.t. neurones the period of recording was long enough to record the responses of the same cell to injections of algesic agents in both the decerebrate and the reversible spinal state. In the reversible spinal state 83% (nineteen out of twenty-three) of the s.c.t. neurones tested with all the three substances responded to at least one of the algesic agents. In the decerebrate state the percentage was lower (39%).5. Reversible spinalization led not only to a significant increase in the number of s.c.t. neurones responding to the algesic agents used but also to an increase in the magnitude of the chemically induced responses.6. The mean latency of the responses of neurones that were activated in both preparations were shorter in the reversible spinal state than in the decerebrate state.7. Control experiments showed that the responses to bradykinin and potassium were entirely due to the nervous outflow from the g.s. muscle. In contrast, intra-arterially applied 5-hydroxytryptamine influenced the s.c.t. cells via unknown additional sites of action.8. The results indicate that muscular group III and/or group IV units excitable by algesic substances do project on to neurones of the spinocervical tract. Furthermore it is concluded that the responses of s.c.t. neurones to activation of fine muscle afferents by algesic agents are subject to a descending control similar to the well known descending modulation of their responsiveness to cutaneous input. Therefore, in addition to serving as a cutaneous pathway the spinocervical tract may take part in muscular nociception.", "contents": "Descending influences on the responses of spinocervical tract neurones to chemical stimulation of fine muscle afferents. 1. In cats, extracellular micro-electrode recordings were made from axons of the spinocervical tract (s.c.t.) in both the decerebrate state and during cold block of the spinal cord (reversible spinal state) to examine the effects of intra-arterial injection of algesic agents (bradykinin, potassium, 5-hydroxytryptamine) into the gastrocnemius-soleus (g.s.) muscle on the discharge behaviour of s.c.t. neurones.2. In the decerebrate state without cooling the spinal cord 13% of the cells (eleven out of eighty-three) responded to intra-arterial injection of bradykinin, 33% (twenty-two out of sixty-nine) to 5-hydroxytryptamine, and 38% (thirty-five out of ninety-one) to potassium injection.3. The general time course and the latency of the responses of s.c.t. cells induced by injection of pain-producing substances into the g.s. muscle reflect in many respects the activations of g.s. group III and group IV primary afferent units studied previously.4. For twenty-seven s.c.t. neurones the period of recording was long enough to record the responses of the same cell to injections of algesic agents in both the decerebrate and the reversible spinal state. In the reversible spinal state 83% (nineteen out of twenty-three) of the s.c.t. neurones tested with all the three substances responded to at least one of the algesic agents. In the decerebrate state the percentage was lower (39%).5. Reversible spinalization led not only to a significant increase in the number of s.c.t. neurones responding to the algesic agents used but also to an increase in the magnitude of the chemically induced responses.6. The mean latency of the responses of neurones that were activated in both preparations were shorter in the reversible spinal state than in the decerebrate state.7. Control experiments showed that the responses to bradykinin and potassium were entirely due to the nervous outflow from the g.s. muscle. In contrast, intra-arterially applied 5-hydroxytryptamine influenced the s.c.t. cells via unknown additional sites of action.8. The results indicate that muscular group III and/or group IV units excitable by algesic substances do project on to neurones of the spinocervical tract. Furthermore it is concluded that the responses of s.c.t. neurones to activation of fine muscle afferents by algesic agents are subject to a descending control similar to the well known descending modulation of their responsiveness to cutaneous input. Therefore, in addition to serving as a cutaneous pathway the spinocervical tract may take part in muscular nociception."}
{"id": "PMID:469742", "title": "Nonlinear spatial summation and the contrast gain control of cat retinal ganglion cells.", "content": "1. We studied how responses to visual stimuli at spatially separated locations were combined by cat retinal ganglion cells. 2. The temporal signal which modulated the stimuli was a sum of sinusoids. Fourier analysis of the ganglion cell impulse train yielded first order responses at the modulation frequencies, and second order responses at sums and differences of the input frequencies. 3. Spatial stimuli were spots in the centre and periphery of the cell's receptive field. Four conditions of stimulation were used: centre alone, periphery alone, centre and periphery in phase, centre and periphery out of phase. 4. The effective first order response of the centre was defined as the response due to centre stimulation in the presence of periphery stimulation, but independent of the relative phases of the two regions. Likewise, the effective first order response of the periphery was defined as the response due to periphery in the presence of centre stimulation, but independent of the relative phases of the two regions. These effective responses may be calculated by addition and subtraction of the measured responses to the combined stimuli. 5. There was a consistent difference between the first order frequency kernal of the effective centre and the first order kernel of the centre alone. The amplitudes of the effective centre responses were diminished at low frequencies of modulation compared to the isolated centre responses. Also, the phase of the effective centre's response to high frequencies was advanced. Such non-linear interaction occurred in all ganglion cells, X or Y, but the effects were larger in Y cells. 6. In addition to spatially uniform stimuli in the periphery, spatial grating patterns were also used. These peripheral gratings affected the first order kernal of the centre even though the peripheral gratings produced no first order responses by themselves. 7. The temporal properties of the non-linear interaction of centre and periphery were probed by modulation in the periphery with single sinusoids. The most effective temporal frequencies for producing non-linear summation were: (a) 4-15 Hz when all the visual stimuli were spatially uniform, (b) 2-8 Hz when spatial grating patterns were used in the periphery. 8. The characteristics of non-linear spatial summation observed in these experiments are explained by the properties of the contrast gain control mechanism which we have previously postulated.", "contents": "Nonlinear spatial summation and the contrast gain control of cat retinal ganglion cells. 1. We studied how responses to visual stimuli at spatially separated locations were combined by cat retinal ganglion cells. 2. The temporal signal which modulated the stimuli was a sum of sinusoids. Fourier analysis of the ganglion cell impulse train yielded first order responses at the modulation frequencies, and second order responses at sums and differences of the input frequencies. 3. Spatial stimuli were spots in the centre and periphery of the cell's receptive field. Four conditions of stimulation were used: centre alone, periphery alone, centre and periphery in phase, centre and periphery out of phase. 4. The effective first order response of the centre was defined as the response due to centre stimulation in the presence of periphery stimulation, but independent of the relative phases of the two regions. Likewise, the effective first order response of the periphery was defined as the response due to periphery in the presence of centre stimulation, but independent of the relative phases of the two regions. These effective responses may be calculated by addition and subtraction of the measured responses to the combined stimuli. 5. There was a consistent difference between the first order frequency kernal of the effective centre and the first order kernel of the centre alone. The amplitudes of the effective centre responses were diminished at low frequencies of modulation compared to the isolated centre responses. Also, the phase of the effective centre's response to high frequencies was advanced. Such non-linear interaction occurred in all ganglion cells, X or Y, but the effects were larger in Y cells. 6. In addition to spatially uniform stimuli in the periphery, spatial grating patterns were also used. These peripheral gratings affected the first order kernal of the centre even though the peripheral gratings produced no first order responses by themselves. 7. The temporal properties of the non-linear interaction of centre and periphery were probed by modulation in the periphery with single sinusoids. The most effective temporal frequencies for producing non-linear summation were: (a) 4-15 Hz when all the visual stimuli were spatially uniform, (b) 2-8 Hz when spatial grating patterns were used in the periphery. 8. The characteristics of non-linear spatial summation observed in these experiments are explained by the properties of the contrast gain control mechanism which we have previously postulated."}
{"id": "PMID:469746", "title": "Segmental and supraspinal input to cells of origin of non-primary fibres in the feline dorsal columns.", "content": "1. The synaptic input to ascending tract cells with axons in the dorsal columns was investigated using intracellular recording. 2. E.p.s.p.s evoked by stimulation of the lateral funiculus were analysed to test for the possibility of collateral connexions between spino-cervical tract cells and dorsal column cells. Three groups of fibres were found to contribute to such e.p.s.p.s: fibres which terminated or originated between spinal segments C3-4 and C1, or Th9 and C3-4 and cortico-spinal tract fibres. The latencies and thresholds of e.p.s.p.s evoked by stimulation of the first group of fibres were compatible with their origin via axon collaterals of spino-cervical tract cells. The occurrence of these e.p.s.p.s in dorsal column cells which were disynaptically excited from cutaneous afferents further corroborated this possibility. 3. E.P.S.P.S of specifically cervical origin were also found in some other neurones in the dorsal horn, probably segmental interneurones, but were absent in spinocervical tract cells. 4. Convergence of group I muscle afferents (possibly both group Ia and group Ib) and cutaneous afferents was found in about 50% of the dorsal column cells. The shortest latency e.p.s.p.s from cutaneous and group I afferents were evoked with segmental delays indicating monosynaptic and disynaptic coupling. 5. I.p.s.p.s were evoked from cutaneous and group I muscle afferents in either the same or different nerves as those from which the e.p.s.p.s were elicited. Excitatory potentials were, however, dominating.", "contents": "Segmental and supraspinal input to cells of origin of non-primary fibres in the feline dorsal columns. 1. The synaptic input to ascending tract cells with axons in the dorsal columns was investigated using intracellular recording. 2. E.p.s.p.s evoked by stimulation of the lateral funiculus were analysed to test for the possibility of collateral connexions between spino-cervical tract cells and dorsal column cells. Three groups of fibres were found to contribute to such e.p.s.p.s: fibres which terminated or originated between spinal segments C3-4 and C1, or Th9 and C3-4 and cortico-spinal tract fibres. The latencies and thresholds of e.p.s.p.s evoked by stimulation of the first group of fibres were compatible with their origin via axon collaterals of spino-cervical tract cells. The occurrence of these e.p.s.p.s in dorsal column cells which were disynaptically excited from cutaneous afferents further corroborated this possibility. 3. E.P.S.P.S of specifically cervical origin were also found in some other neurones in the dorsal horn, probably segmental interneurones, but were absent in spinocervical tract cells. 4. Convergence of group I muscle afferents (possibly both group Ia and group Ib) and cutaneous afferents was found in about 50% of the dorsal column cells. The shortest latency e.p.s.p.s from cutaneous and group I afferents were evoked with segmental delays indicating monosynaptic and disynaptic coupling. 5. I.p.s.p.s were evoked from cutaneous and group I muscle afferents in either the same or different nerves as those from which the e.p.s.p.s were elicited. Excitatory potentials were, however, dominating."}
{"id": "PMID:469751", "title": "Three-micro-electrode voltage clamp experiments in calf cardiac Purkinje fibres: is slow inward current adequately measured?", "content": "1. The three-micro-electrode voltage clamp method (Adrian, Chandler & Hodgkin, 1970) was adapted for the study of regenerative inward currents in cardiac muscle. The adequacy of measurements of slow inward current in cardiac Purkinje fibres was assessed. 2. Membrane current density is reported simultaneously by total applied current (IT), along with a longitudinal voltage difference signal (delta V), recorded between two intracellular micro-electrodes. 3. Non-linear cable calculations show that delta V is a more faithful measure of membrane current density than IT as peak inward current increases. Quantitative agreement between delta V and IT only occurs when both signals report the membrane characteristics that would be obtained with an ideal longitudinal space clamp. 4. Agreement between delta V and IT is thus a useful criterion for satisfactory experimental measurements which we applied to the slow inward current. This component was elicited by depolarizing steps from a holding potential near =45 mV in the presence of tetrodotoxin. The IT signal was compared directly with delta V/R, where R is an effective longitudinal resistance that was experimentally determined. 5. delta V/R and IT showed very good agreement in both peak amplitude and time course at all potentials studied. 6. Radial non-uniformity during the measured peak slow inward current was estimated by calculations assuming clefts 200 A wide with a uniform distribution of ionic channels. The calculated voltage span from surface to centre was always less than 5 mV, and the measured I-V characteristics showed little distortion. 7. In another check, I-V characteristics and slow response membrane action potentials were compared. The measured peak current showed good agreement with the product (total preparation capacitance) x (rate of rise). 8. The experimental and theoretical analysis suggest that the measurements of slow inward current are a good approximation to genuine membrane properties.", "contents": "Three-micro-electrode voltage clamp experiments in calf cardiac Purkinje fibres: is slow inward current adequately measured? 1. The three-micro-electrode voltage clamp method (Adrian, Chandler & Hodgkin, 1970) was adapted for the study of regenerative inward currents in cardiac muscle. The adequacy of measurements of slow inward current in cardiac Purkinje fibres was assessed. 2. Membrane current density is reported simultaneously by total applied current (IT), along with a longitudinal voltage difference signal (delta V), recorded between two intracellular micro-electrodes. 3. Non-linear cable calculations show that delta V is a more faithful measure of membrane current density than IT as peak inward current increases. Quantitative agreement between delta V and IT only occurs when both signals report the membrane characteristics that would be obtained with an ideal longitudinal space clamp. 4. Agreement between delta V and IT is thus a useful criterion for satisfactory experimental measurements which we applied to the slow inward current. This component was elicited by depolarizing steps from a holding potential near =45 mV in the presence of tetrodotoxin. The IT signal was compared directly with delta V/R, where R is an effective longitudinal resistance that was experimentally determined. 5. delta V/R and IT showed very good agreement in both peak amplitude and time course at all potentials studied. 6. Radial non-uniformity during the measured peak slow inward current was estimated by calculations assuming clefts 200 A wide with a uniform distribution of ionic channels. The calculated voltage span from surface to centre was always less than 5 mV, and the measured I-V characteristics showed little distortion. 7. In another check, I-V characteristics and slow response membrane action potentials were compared. The measured peak current showed good agreement with the product (total preparation capacitance) x (rate of rise). 8. The experimental and theoretical analysis suggest that the measurements of slow inward current are a good approximation to genuine membrane properties."}
{"id": "PMID:469755", "title": "Tetanus toxin and synaptic inhibition in the substantia nigra and striatum of the rat.", "content": "1. The effects of tetanus toxin were determined on GABA-mediated synaptic inhibition of substantia nigra neurones evoked by striatal stimulation and on the presumed dopamine- and 5-hydroxytryptamine-mediated synaptic inhibition of striatal neurones evoked by nigral and dorsal raphe nucleus stimulation, respectively, in the urethane-anaesthetized rat. 2. Following an intranigral injection of tetanus toxin, striatal-evoked inhibition of substantia nigra neurones, which is sensitive to bicuculline, was rapidly abolished. This effect was not accompanied by any significant change in the responses of nigral neurones to ionophoretically administered GABA or other putative neurotransmitters and thus indicates a presynaptic site of action of the toxin. 3. The rate of onset of action of the toxin in the substantia nigra was extremely rapid (1-4 min) and appeared to be related to the rate of activation of the inhibitory pathway. 4. Injections into the substantia nigra of tetanus toxin neutralized with antitoxin had no significant effect on striatal-evoked inhibition in the substantia nigra. 5. Injections of tetanus toxin into the striatum failed to influence the inhibition of striatal neurones evoked by stimulation of the ipsilateral substantia nigra or the dorsal raphe nucleus, suggesting that tetanus toxin does not impair monoamine-mediated inhibition in the central nervous system. 6. Synaptic excitation which preceded substantia-nigra-evoked inhibition in striatal neurones and which occasionally preceded striatal-evoked inhibition in nigral neurones was also unaffected by tetanus toxin. 7. It is suggested that tetanus toxin selectively abolishes GABA-mediated synaptic inhibition in the central nervous system and may be a useful tool in the identification of such synaptic inhibitory mechanisms.", "contents": "Tetanus toxin and synaptic inhibition in the substantia nigra and striatum of the rat. 1. The effects of tetanus toxin were determined on GABA-mediated synaptic inhibition of substantia nigra neurones evoked by striatal stimulation and on the presumed dopamine- and 5-hydroxytryptamine-mediated synaptic inhibition of striatal neurones evoked by nigral and dorsal raphe nucleus stimulation, respectively, in the urethane-anaesthetized rat. 2. Following an intranigral injection of tetanus toxin, striatal-evoked inhibition of substantia nigra neurones, which is sensitive to bicuculline, was rapidly abolished. This effect was not accompanied by any significant change in the responses of nigral neurones to ionophoretically administered GABA or other putative neurotransmitters and thus indicates a presynaptic site of action of the toxin. 3. The rate of onset of action of the toxin in the substantia nigra was extremely rapid (1-4 min) and appeared to be related to the rate of activation of the inhibitory pathway. 4. Injections into the substantia nigra of tetanus toxin neutralized with antitoxin had no significant effect on striatal-evoked inhibition in the substantia nigra. 5. Injections of tetanus toxin into the striatum failed to influence the inhibition of striatal neurones evoked by stimulation of the ipsilateral substantia nigra or the dorsal raphe nucleus, suggesting that tetanus toxin does not impair monoamine-mediated inhibition in the central nervous system. 6. Synaptic excitation which preceded substantia-nigra-evoked inhibition in striatal neurones and which occasionally preceded striatal-evoked inhibition in nigral neurones was also unaffected by tetanus toxin. 7. It is suggested that tetanus toxin selectively abolishes GABA-mediated synaptic inhibition in the central nervous system and may be a useful tool in the identification of such synaptic inhibitory mechanisms."}
{"id": "PMID:469754", "title": "Electrical properties associated with wide intercellular clefts in rabbit Purkinje fibres.", "content": "1. Rabbit Purkinje fibres were studied using micro-electrode recordings of electrical activity or a two-micro-electrode voltage clamp. Previous morphological work had suggested that these preparations offer structural advantages for the analysis of ionic permeability mechanisms. 2. Viable preparations could be obtained consistently by exposure to a K glutamate Tyrode solution during excision and recovery. In NaCl Tyrode solution, the action potential showed a large overshoot and fully developed plateau, but no pacemaker depolarization at negative potentials. 3. The passive electrical properties were consistent with morphological evidence for the accessibility of cleft membranes within the cell bundle. Electrotonic responses to intracellular current steps showed the behaviour expected for a simple leaky capacitative cable. Capacitative current transients under voltage clamp were changed very little by an eightfold reduction in the external solution conductivity. 4. Slow current changes attributable to K depletion were small compared to those found in other cardiac preparations. The amount of depletion was close to that predicted by a cleft model which assumed free K diffusion in 1 micron clefts. 5. Step depolarizations over the plateau range of potentials evoked a slow inward current which was resistant to tetrodotoxin but blocked by D600. 6. Strong depolarizations to potentials near 0 mV elicited a transient outward current and a slowly activating late outward current. Both components resembled currents found in sheep or calf Purkinje fibres. 7. These experiments support previous interpretations of slow plateau currents in terms of genuine permeability changes. The rabbit Purkinje fibre may allow various ionic channels to be studied with relatively little interference from radial non-uniformities in membrane potential or ion concentration.", "contents": "Electrical properties associated with wide intercellular clefts in rabbit Purkinje fibres. 1. Rabbit Purkinje fibres were studied using micro-electrode recordings of electrical activity or a two-micro-electrode voltage clamp. Previous morphological work had suggested that these preparations offer structural advantages for the analysis of ionic permeability mechanisms. 2. Viable preparations could be obtained consistently by exposure to a K glutamate Tyrode solution during excision and recovery. In NaCl Tyrode solution, the action potential showed a large overshoot and fully developed plateau, but no pacemaker depolarization at negative potentials. 3. The passive electrical properties were consistent with morphological evidence for the accessibility of cleft membranes within the cell bundle. Electrotonic responses to intracellular current steps showed the behaviour expected for a simple leaky capacitative cable. Capacitative current transients under voltage clamp were changed very little by an eightfold reduction in the external solution conductivity. 4. Slow current changes attributable to K depletion were small compared to those found in other cardiac preparations. The amount of depletion was close to that predicted by a cleft model which assumed free K diffusion in 1 micron clefts. 5. Step depolarizations over the plateau range of potentials evoked a slow inward current which was resistant to tetrodotoxin but blocked by D600. 6. Strong depolarizations to potentials near 0 mV elicited a transient outward current and a slowly activating late outward current. Both components resembled currents found in sheep or calf Purkinje fibres. 7. These experiments support previous interpretations of slow plateau currents in terms of genuine permeability changes. The rabbit Purkinje fibre may allow various ionic channels to be studied with relatively little interference from radial non-uniformities in membrane potential or ion concentration."}
{"id": "PMID:469759", "title": "The initiation and maintenance of bradycardia in a diving mammal, the muskrat, Ondatra zibethica.", "content": "1. The cardiac and respiratory responses shown by muskrats in both unrestrained and restrained dives have been compared with responses elicited by stimulation of a number of cardio-depressant receptor inputs, in an attempt to determine which are most important in initiating and maintaining diving bradycardia. 2. In unrestrained voluntary dives heart rate fell from 310 +/- 3 to 54 +/- 3 beats min-1 in 1 to 2 sec, which was significantly below that seen in dives by restrained unanaesthetized or anaesthetized animals. 3. Pouring water on the external nares during maintained artificial ventilation caused heart rate to decline to 76 +/- 12 beats min-1 after 1 sec. Flowing water through the internal nares caused apnoea, in the expiratory position, and bradycardia within one third of a second. Heart rate fell to 20 +/- 2 beats min-1, 1 sec after the start of water flow. Substituting saline for water reduced both the apnoeic and cardiac responses. Bilateral section of the maxillary branch of V and the inferior laryngeal (X) nerves completely abolished the cardiac and respiratory response to water flow. 4. Artificial ventilation throughout periods of nasal stimulation with water or saline reduced the bradycardia, although even the saline driven response could not be completely abolished. Lung deafferentation eliminated any direct effect of artificial ventilation on heart rate during nasal stimulation. 5. Lung deflation caused bradycardia within 0.97 +/- 0.17 sec, heart rate falling from 268 +/- 7 to 59 +/- 4 beats min-1. Bradycardia also occurred during maintained lung inflation but it was delayed for a period which varied from 6.8 +/- 1.8 sec at an inflation pressure of 0.5 kPa to 35 +/- 7 sec at 1.5 kPa. 6. Bradycardia caused by nasal water flow or lung deflation was unaffected by bilateral section of the sinus nerve. 7. Artificial ventilation of paralysed muskrats with 5% CO2 in N2 caused bradycardia when Pa, O2 reached 8.4 +/- 0.8 kPa and heart rate declined to 76 +/- 7 beats min-1 at 4 kPa. Bilateral section of the sinus nerve delayed bradycardia until Pa, O2 reached 4.5 +/- 0.5 kPa. 8. These results suggest that the cardiac response to submergence could be the expression of input from three groups of receptors, nasal, lung and carotid chemoreceptors, although it is not clear how they interact with one another to generate the cardiac responses displayed by unrestrained animals during submergence.", "contents": "The initiation and maintenance of bradycardia in a diving mammal, the muskrat, Ondatra zibethica. 1. The cardiac and respiratory responses shown by muskrats in both unrestrained and restrained dives have been compared with responses elicited by stimulation of a number of cardio-depressant receptor inputs, in an attempt to determine which are most important in initiating and maintaining diving bradycardia. 2. In unrestrained voluntary dives heart rate fell from 310 +/- 3 to 54 +/- 3 beats min-1 in 1 to 2 sec, which was significantly below that seen in dives by restrained unanaesthetized or anaesthetized animals. 3. Pouring water on the external nares during maintained artificial ventilation caused heart rate to decline to 76 +/- 12 beats min-1 after 1 sec. Flowing water through the internal nares caused apnoea, in the expiratory position, and bradycardia within one third of a second. Heart rate fell to 20 +/- 2 beats min-1, 1 sec after the start of water flow. Substituting saline for water reduced both the apnoeic and cardiac responses. Bilateral section of the maxillary branch of V and the inferior laryngeal (X) nerves completely abolished the cardiac and respiratory response to water flow. 4. Artificial ventilation throughout periods of nasal stimulation with water or saline reduced the bradycardia, although even the saline driven response could not be completely abolished. Lung deafferentation eliminated any direct effect of artificial ventilation on heart rate during nasal stimulation. 5. Lung deflation caused bradycardia within 0.97 +/- 0.17 sec, heart rate falling from 268 +/- 7 to 59 +/- 4 beats min-1. Bradycardia also occurred during maintained lung inflation but it was delayed for a period which varied from 6.8 +/- 1.8 sec at an inflation pressure of 0.5 kPa to 35 +/- 7 sec at 1.5 kPa. 6. Bradycardia caused by nasal water flow or lung deflation was unaffected by bilateral section of the sinus nerve. 7. Artificial ventilation of paralysed muskrats with 5% CO2 in N2 caused bradycardia when Pa, O2 reached 8.4 +/- 0.8 kPa and heart rate declined to 76 +/- 7 beats min-1 at 4 kPa. Bilateral section of the sinus nerve delayed bradycardia until Pa, O2 reached 4.5 +/- 0.5 kPa. 8. These results suggest that the cardiac response to submergence could be the expression of input from three groups of receptors, nasal, lung and carotid chemoreceptors, although it is not clear how they interact with one another to generate the cardiac responses displayed by unrestrained animals during submergence."}
{"id": "PMID:469765", "title": "Pancreatic acinar cells: effects of micro-ionophoretic polypeptide application on membrane potential and resistance.", "content": "1. Acinar cell membrane potential and resistance were measured from superfused segments of mouse pancreas, in vitro, using intracellular glass micro-electrodes. One or two extracellular micropipettes containing caerulein, bombesin nonapeptide (Bn) or acetylcholine (ACh) were placed near to the surface of the impaled acinus. The secretagogues were ejected rapidly from the micropipettes by ionophoresis.2. Each secretagogue evoked a similar electrical response from the impaled acinar cell: membrane depolarization and a simultaneous reduction in input resistance. The duration of cell activation from caerulein ionophoresis was longer than that observed for ACh and Bn. The cell response to the peptide hormone applications could be repeated in the presence of atropine.3. The minimum interval before the onset of cell depolarization after caerulein ionophoresis was determined. Values ranged between 500 and 1000 msec. The minimum latencies after Bn ionophoresis were 500-1400 msec.4. With two electrodes inserted into electrically coupled acinar cells, direct measurements of the caerulein and Bn null potentials were made. At high negative membrane potentials an enhanced depolarization was evoked by caerulein ionophoresis. At low negative membrane potentials the caerulein stimulation produced a diminished depolarization, and at membrane potentials less than - 10 mV acinar cell hyperpolarizations were observed. A similar series of responses was obtained in experiments where Bn ionophoresis was used. The caerulein and the Bn null potentials were always contained within - 10 to - 15 mV.5. The results describe the almost identical electrical response of acinar cells to stimulation by ACh, caerulein and bombesin. All three secretagogues have similar null potentials and latencies of activation on acinar cells. The bombesin latency responses appear as short as those measured for caerulein and provide electro-physiological evidence that Bn acts directly on acinar cells. The findings support the hypothesis that ACh, caerulein and Bn, though acting on different receptors, evoke the observed changes in electrical properties of acinar cell membranes, through a common pathway.", "contents": "Pancreatic acinar cells: effects of micro-ionophoretic polypeptide application on membrane potential and resistance. 1. Acinar cell membrane potential and resistance were measured from superfused segments of mouse pancreas, in vitro, using intracellular glass micro-electrodes. One or two extracellular micropipettes containing caerulein, bombesin nonapeptide (Bn) or acetylcholine (ACh) were placed near to the surface of the impaled acinus. The secretagogues were ejected rapidly from the micropipettes by ionophoresis.2. Each secretagogue evoked a similar electrical response from the impaled acinar cell: membrane depolarization and a simultaneous reduction in input resistance. The duration of cell activation from caerulein ionophoresis was longer than that observed for ACh and Bn. The cell response to the peptide hormone applications could be repeated in the presence of atropine.3. The minimum interval before the onset of cell depolarization after caerulein ionophoresis was determined. Values ranged between 500 and 1000 msec. The minimum latencies after Bn ionophoresis were 500-1400 msec.4. With two electrodes inserted into electrically coupled acinar cells, direct measurements of the caerulein and Bn null potentials were made. At high negative membrane potentials an enhanced depolarization was evoked by caerulein ionophoresis. At low negative membrane potentials the caerulein stimulation produced a diminished depolarization, and at membrane potentials less than - 10 mV acinar cell hyperpolarizations were observed. A similar series of responses was obtained in experiments where Bn ionophoresis was used. The caerulein and the Bn null potentials were always contained within - 10 to - 15 mV.5. The results describe the almost identical electrical response of acinar cells to stimulation by ACh, caerulein and bombesin. All three secretagogues have similar null potentials and latencies of activation on acinar cells. The bombesin latency responses appear as short as those measured for caerulein and provide electro-physiological evidence that Bn acts directly on acinar cells. The findings support the hypothesis that ACh, caerulein and Bn, though acting on different receptors, evoke the observed changes in electrical properties of acinar cell membranes, through a common pathway."}
{"id": "PMID:469769", "title": "The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.", "content": "1. Theophylline (10 mM) and choleragen change the direction of net Cl- movements across rabbit ileum, in the short-circuit current condition, from absorption to secretion. The specific activity ratio R of Cl- tracers within the tissue coming from mucosal and serosal solutions respectively is increased, which is consistent with an increase in Cl- exchange flux across the mucosal border. 2. Net Na+ movement is also changed from net absorption to secretion by theophylline and choleragen; the specific activity ratio R of Na+ tracers is raised by theophylline. Because of the large paracellular component to transepithelial Na+ movements, an increase in Na+ exchange flux across the mucosal border is not detected. 3. 2,4,6-Triaminopyrimidine (20 mM) which has been previously shown to block paracellular Na+ movements, blocks both the theophylline and choleragen-dependent reversal of net Na+ movement by preventing the decrease in m-s Na flux. The theophylline-dependent increase in the ratio R of Na+ is still present, and is consistent with an increase in Na+ exchange flux across the mucosal border--unmasked by removal of the paracellular flux components. 4. Ouabain (0.1 mM) abolishes net absorption of Na+ and Cl- in control and net secretion of Na+ and Cl- in theophylline-treated tissue. Ouabain does not affect the theophylline-dependent increase in Cl- exchange across the mucosal border. 5. Replacement of Ringer Cl- with SO24- or Na+ by choline prevents the effects of theophylline and choleragen on Na+ and Cl- fluxes respectively. 6. Ethacrynate (0.1 mM) prevents the theophylline-dependent effects on net Na+ movement. Raising ethacrynate to 0.2 mM abolishes the effects of theophylline on Cl- exchange. An interpretation of these results is that theophylline and choleragen raise the Cl- permeability of the brush border. This increases NaCl leakage from the hypertonic lateral intercellular space into the mucosal solution thereby causing secretion. The selective action of triaminopyrimidine and ethacrynate (0.1 mM) on Na+ flux indicates that Na+ and Cl- move via separate transport pathways across the mucosal border.", "contents": "The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum. 1. Theophylline (10 mM) and choleragen change the direction of net Cl- movements across rabbit ileum, in the short-circuit current condition, from absorption to secretion. The specific activity ratio R of Cl- tracers within the tissue coming from mucosal and serosal solutions respectively is increased, which is consistent with an increase in Cl- exchange flux across the mucosal border. 2. Net Na+ movement is also changed from net absorption to secretion by theophylline and choleragen; the specific activity ratio R of Na+ tracers is raised by theophylline. Because of the large paracellular component to transepithelial Na+ movements, an increase in Na+ exchange flux across the mucosal border is not detected. 3. 2,4,6-Triaminopyrimidine (20 mM) which has been previously shown to block paracellular Na+ movements, blocks both the theophylline and choleragen-dependent reversal of net Na+ movement by preventing the decrease in m-s Na flux. The theophylline-dependent increase in the ratio R of Na+ is still present, and is consistent with an increase in Na+ exchange flux across the mucosal border--unmasked by removal of the paracellular flux components. 4. Ouabain (0.1 mM) abolishes net absorption of Na+ and Cl- in control and net secretion of Na+ and Cl- in theophylline-treated tissue. Ouabain does not affect the theophylline-dependent increase in Cl- exchange across the mucosal border. 5. Replacement of Ringer Cl- with SO24- or Na+ by choline prevents the effects of theophylline and choleragen on Na+ and Cl- fluxes respectively. 6. Ethacrynate (0.1 mM) prevents the theophylline-dependent effects on net Na+ movement. Raising ethacrynate to 0.2 mM abolishes the effects of theophylline on Cl- exchange. An interpretation of these results is that theophylline and choleragen raise the Cl- permeability of the brush border. This increases NaCl leakage from the hypertonic lateral intercellular space into the mucosal solution thereby causing secretion. The selective action of triaminopyrimidine and ethacrynate (0.1 mM) on Na+ flux indicates that Na+ and Cl- move via separate transport pathways across the mucosal border."}
{"id": "PMID:469772", "title": "Fluid movements across rabbit ileum coupled to passive paracellular ion movements.", "content": "1. Theophylline (10 mM) and choleragen (1 x 10(-6) g ml.-1) abolish net fluid absorption by everted sacs of rabbit ileum. Triaminopyrimidine (20 mM) and ethacrynate (0.1 mM) prevent this inhibition of net fluid movement. Replacing Ringer Cl- with isethionate prevents the theophylline-dependent decrease in fluid absorption also. 2. Ouabain (0.1 mM) abolishes net fluid movements in both control and theophylline-treated tissue. 3. With ouabain present, hypertonic NaCl (200 mM) in the mucosal solution causes net fluid secretion (serosal-mucosal flux). With theophylline added to both the mucosal and serosal solution, net fluid absorption (mucosal-serosal flux) is observed (P less than 0.001). Triaminopyrimidine (20 mM), or ethacrynate (0.1 mM), or replacement of Ringer Na+ with choline, or Ringer Cl- with isethionate all prevent the theophylline-induced reversal of osmotic flow. 4. Theophylline increases passive net flux of Na+ and Cl- from mucosal solution containing hypertonic (200 mM) NaCl+ ouabain (0.1 mM) across sheets of ileum into serosal solution containing mannitol Ringer + ouabain. The increased passive Na+ flux is blocked by triaminopyrimidine and the increased Na+ and Cl- fluxes are blocked by ethacrynate (0.1 mM). 5. The suggested route of increased NaCl leakage is via the paracellular pathway as it is inhibited by triaminopyrimidine. The increase, itself, is a consequence of the increased passive permeability of the mucosal border to Cl-, induced by theophylline or choleragen. Water is apparently electro-osmotically coupled to the paracellular Na+ leakage (100 mole water mole-1 Na+), hence increased passive leakage reverses osmotic flow. In active tissue the lateral intercellular space contains hypertonic NaCl, and hence increased leakage of NaCl across the tight-junction in theophylline or choleragen-treated tissue gives rise to net fluid secretion.", "contents": "Fluid movements across rabbit ileum coupled to passive paracellular ion movements. 1. Theophylline (10 mM) and choleragen (1 x 10(-6) g ml.-1) abolish net fluid absorption by everted sacs of rabbit ileum. Triaminopyrimidine (20 mM) and ethacrynate (0.1 mM) prevent this inhibition of net fluid movement. Replacing Ringer Cl- with isethionate prevents the theophylline-dependent decrease in fluid absorption also. 2. Ouabain (0.1 mM) abolishes net fluid movements in both control and theophylline-treated tissue. 3. With ouabain present, hypertonic NaCl (200 mM) in the mucosal solution causes net fluid secretion (serosal-mucosal flux). With theophylline added to both the mucosal and serosal solution, net fluid absorption (mucosal-serosal flux) is observed (P less than 0.001). Triaminopyrimidine (20 mM), or ethacrynate (0.1 mM), or replacement of Ringer Na+ with choline, or Ringer Cl- with isethionate all prevent the theophylline-induced reversal of osmotic flow. 4. Theophylline increases passive net flux of Na+ and Cl- from mucosal solution containing hypertonic (200 mM) NaCl+ ouabain (0.1 mM) across sheets of ileum into serosal solution containing mannitol Ringer + ouabain. The increased passive Na+ flux is blocked by triaminopyrimidine and the increased Na+ and Cl- fluxes are blocked by ethacrynate (0.1 mM). 5. The suggested route of increased NaCl leakage is via the paracellular pathway as it is inhibited by triaminopyrimidine. The increase, itself, is a consequence of the increased passive permeability of the mucosal border to Cl-, induced by theophylline or choleragen. Water is apparently electro-osmotically coupled to the paracellular Na+ leakage (100 mole water mole-1 Na+), hence increased passive leakage reverses osmotic flow. In active tissue the lateral intercellular space contains hypertonic NaCl, and hence increased leakage of NaCl across the tight-junction in theophylline or choleragen-treated tissue gives rise to net fluid secretion."}
{"id": "PMID:469773", "title": "Electrophysiological and electron-microscopical correlations with fluid and electrolyte secretion in rabbit ileum.", "content": "1. The effects of theophylline and triaminopyrimidine on the passive permeability of Na+ and Cl- across sheets of rabbit ileum treated with 0.1 mM-ouabain were examined by determining the NaCl: mannitol dilution potentials, K+:Na+; choline: Na+ and SO24-:Cl- biionic potentials. The results indicate (a) that triaminopyrimidine reduces paracellular Na+ and K+ permeability without affecting Cl- permeability and (b) that theophylline increases Cl- permeability without affecting Na+ permeability and (c) that neither theophylline nor triaminopyrimidine interfere with each other's action. This is further evidence consistent with separate routes for paracellular Na+ and Cl- movement. 2. Electrical resistance changes across sheets of actively transporting rabbit ileum were measured as a function of time in various conditions. Theophylline has a biphasic effect on resistance. Initially it decreases resistance from 56 omega cm2 (control) to 40 omega cm2. In the ensuing 30 min, resistance rises to 50 omega cm2; whereas it falls to 30 omega cm2 in controls. When theophylline is present, with triaminopyrimidine, or with galactose, no secondary rise in tissue resistance occurs. The initial decrease in resistance is consistent with a theophylline-dependent increase in Cl- conductance and the secondary rise in resistance may be attributed to collapse of the lateral intercellular space following leakage of NaCl and fluid into the mucosal solution. 3. Electron microscopy of glutaraldehyde-fixed tissue confirms the above views, since, with theophylline present, the lateral intercellular spaces are collapsed and with both triaminopyrimidine and theophylline, or both theophylline and 20 mM-galactose present the spaces remain open. 4. It is shown in the Discussion that the theophylline- or choleragen induced increase in passive Cl- permeability of the mucosal border is the only requirement necessary to explain the increase in electrogenic Cl- secretion, the increase in short-circuit current, as well as neutral secretion of NaCl and net fluid secretion.", "contents": "Electrophysiological and electron-microscopical correlations with fluid and electrolyte secretion in rabbit ileum. 1. The effects of theophylline and triaminopyrimidine on the passive permeability of Na+ and Cl- across sheets of rabbit ileum treated with 0.1 mM-ouabain were examined by determining the NaCl: mannitol dilution potentials, K+:Na+; choline: Na+ and SO24-:Cl- biionic potentials. The results indicate (a) that triaminopyrimidine reduces paracellular Na+ and K+ permeability without affecting Cl- permeability and (b) that theophylline increases Cl- permeability without affecting Na+ permeability and (c) that neither theophylline nor triaminopyrimidine interfere with each other's action. This is further evidence consistent with separate routes for paracellular Na+ and Cl- movement. 2. Electrical resistance changes across sheets of actively transporting rabbit ileum were measured as a function of time in various conditions. Theophylline has a biphasic effect on resistance. Initially it decreases resistance from 56 omega cm2 (control) to 40 omega cm2. In the ensuing 30 min, resistance rises to 50 omega cm2; whereas it falls to 30 omega cm2 in controls. When theophylline is present, with triaminopyrimidine, or with galactose, no secondary rise in tissue resistance occurs. The initial decrease in resistance is consistent with a theophylline-dependent increase in Cl- conductance and the secondary rise in resistance may be attributed to collapse of the lateral intercellular space following leakage of NaCl and fluid into the mucosal solution. 3. Electron microscopy of glutaraldehyde-fixed tissue confirms the above views, since, with theophylline present, the lateral intercellular spaces are collapsed and with both triaminopyrimidine and theophylline, or both theophylline and 20 mM-galactose present the spaces remain open. 4. It is shown in the Discussion that the theophylline- or choleragen induced increase in passive Cl- permeability of the mucosal border is the only requirement necessary to explain the increase in electrogenic Cl- secretion, the increase in short-circuit current, as well as neutral secretion of NaCl and net fluid secretion."}
{"id": "PMID:469774", "title": "The response characteristics of an inhibitory binocular interaction in human vision.", "content": "1. The contrast threshold level for visual detection of a linear grating consisting of parallel light and dark bars is increased by adaptation to a high contrast grating of spatial characteristics similar to those of the test grating (e.g. Blakemore & Campbell, 1969). This so-called contrast threshold elevation effect is significantly reduced if two adaptation gratings, with appropriate different spatial characteristics, are presented one to either eye (Ruddock & Wigley, 1976). We have studied the contrast threshold elevation effect obtained with a test and an adaptation grating presented to one eye and a second adaptation grating, referred to as the conditioning grating, presented to the other. 2. Preliminary data are presented for three subjects with normal stereoscopic vision. In each case, the contrast threshold elevation effect for a pair of spatially identical test and adaptation gratings is significantly reduced by a conditioning grating of spatial frequency in the range 1-5 c/deg. 3. Reduction in the contrast elevation effect is observed whether the conditioning and adaptation gratings are of the same or of different wave-lengths and the effect of the conditioning grating increases to a steady-state level over a period of some 30 sec following onset of its presentation. 4. Measurements were made with a 5 deg diameter circular test grating presented at retinal locations up to 8 deg in the horizontal and vertical meridians from a foveal fixation point. It was found that in the horizontal meridian, the amplitude of the suppression effect associated with the conditioning grating falls as the displacement angle of the test field from the fixation point increases. For displacements in the vertical meridian, however, the amplitude of the suppression effect is virtually independent of the retinal location of the test field. 5. Under experimental conditions which yield suppression of the contrast threshold elevation effect for subjects with normal stereoscopic vision, no suppression was found for three subjects who possessed neither global nor fine stereoscopic vision. Results for a subject who possessed fine, but not global stereoscopy, did, however, show the suppression effect.", "contents": "The response characteristics of an inhibitory binocular interaction in human vision. 1. The contrast threshold level for visual detection of a linear grating consisting of parallel light and dark bars is increased by adaptation to a high contrast grating of spatial characteristics similar to those of the test grating (e.g. Blakemore & Campbell, 1969). This so-called contrast threshold elevation effect is significantly reduced if two adaptation gratings, with appropriate different spatial characteristics, are presented one to either eye (Ruddock & Wigley, 1976). We have studied the contrast threshold elevation effect obtained with a test and an adaptation grating presented to one eye and a second adaptation grating, referred to as the conditioning grating, presented to the other. 2. Preliminary data are presented for three subjects with normal stereoscopic vision. In each case, the contrast threshold elevation effect for a pair of spatially identical test and adaptation gratings is significantly reduced by a conditioning grating of spatial frequency in the range 1-5 c/deg. 3. Reduction in the contrast elevation effect is observed whether the conditioning and adaptation gratings are of the same or of different wave-lengths and the effect of the conditioning grating increases to a steady-state level over a period of some 30 sec following onset of its presentation. 4. Measurements were made with a 5 deg diameter circular test grating presented at retinal locations up to 8 deg in the horizontal and vertical meridians from a foveal fixation point. It was found that in the horizontal meridian, the amplitude of the suppression effect associated with the conditioning grating falls as the displacement angle of the test field from the fixation point increases. For displacements in the vertical meridian, however, the amplitude of the suppression effect is virtually independent of the retinal location of the test field. 5. Under experimental conditions which yield suppression of the contrast threshold elevation effect for subjects with normal stereoscopic vision, no suppression was found for three subjects who possessed neither global nor fine stereoscopic vision. Results for a subject who possessed fine, but not global stereoscopy, did, however, show the suppression effect."}
{"id": "PMID:469777", "title": "Electromechanical noise in atrial muscle cells of the carp: a possible ionic feed-back mechanism.", "content": "1. Spontaneous electrical fluctuations (+/- 0.2-0.4 mV) were recorded in 'quiescent' atrial fibres of the carp. 2. The noise decreased in acetylcholine, small hyperpolarization and EGTA. 3. The noise increased (to +/- 1-2 mV) and became more synchronous in K+-free Ringer and in ouabain. 4. Large voltage fluctuations (+/- 1-2 mV) were accompanied by a fine mechanical tremor, indicating intracellular [Ca2+] fluctuations. 5. Spectral analysis showed a clear resonant frequency at about 1 Hz, indicating that the noise cannot result from the random switching of independent ionic channels. 6. We propose that the intracellular [Ca2+] and the membrane K+-conductance are involved in a feed-back loop which can oscillate and produce the electromechanical noise. The frequency of oscillation is determined by the relatively slow diffusion of Ca2+ from the intracellular reservoir to the surface membrane.", "contents": "Electromechanical noise in atrial muscle cells of the carp: a possible ionic feed-back mechanism. 1. Spontaneous electrical fluctuations (+/- 0.2-0.4 mV) were recorded in 'quiescent' atrial fibres of the carp. 2. The noise decreased in acetylcholine, small hyperpolarization and EGTA. 3. The noise increased (to +/- 1-2 mV) and became more synchronous in K+-free Ringer and in ouabain. 4. Large voltage fluctuations (+/- 1-2 mV) were accompanied by a fine mechanical tremor, indicating intracellular [Ca2+] fluctuations. 5. Spectral analysis showed a clear resonant frequency at about 1 Hz, indicating that the noise cannot result from the random switching of independent ionic channels. 6. We propose that the intracellular [Ca2+] and the membrane K+-conductance are involved in a feed-back loop which can oscillate and produce the electromechanical noise. The frequency of oscillation is determined by the relatively slow diffusion of Ca2+ from the intracellular reservoir to the surface membrane."}
{"id": "PMID:469778", "title": "Hormones, feeding and temperature in sheep following cerebroventricular injections of neurotransmitters and carbachol.", "content": "1. The neural mechanisms involved in the control of prolactin and growth hormone secretion, body temperature and feeding in castrated male sheep were investigated by the lateral cerebroventricular injection of several putative neurotransmitters and carbachol. 2. Feeding was inhibited by 10 and 20 microgram carbachol and by 400 microgram histamine. 100 microgram Noradrenaline stimulated, but 200 microgram inhibited, feeding; however, both  results were non-significant compared with controls. 3. 20 microgram Carbachol elicited a highly significant rise in plasma growth hormone, suggesting a cholinergic component in the neural control of growth hormone in sheep. Noradrenaline, dopamine and 5-hydroxytryptamine, but not melatonin, significantly depressed plasma prolactin concentration indicating a similar neural control for the hormone's release in sheep as in other species. 4. Deep body temperature appeared unaffected by any ofthe injected drugs. 5. The possible interrelationships between feeding behaviour and the circulating levels of growth hormone in ruminants is discussed. 6. A method for the radioimmunoassay of ovine growth hormone is described and validated.", "contents": "Hormones, feeding and temperature in sheep following cerebroventricular injections of neurotransmitters and carbachol. 1. The neural mechanisms involved in the control of prolactin and growth hormone secretion, body temperature and feeding in castrated male sheep were investigated by the lateral cerebroventricular injection of several putative neurotransmitters and carbachol. 2. Feeding was inhibited by 10 and 20 microgram carbachol and by 400 microgram histamine. 100 microgram Noradrenaline stimulated, but 200 microgram inhibited, feeding; however, both  results were non-significant compared with controls. 3. 20 microgram Carbachol elicited a highly significant rise in plasma growth hormone, suggesting a cholinergic component in the neural control of growth hormone in sheep. Noradrenaline, dopamine and 5-hydroxytryptamine, but not melatonin, significantly depressed plasma prolactin concentration indicating a similar neural control for the hormone's release in sheep as in other species. 4. Deep body temperature appeared unaffected by any ofthe injected drugs. 5. The possible interrelationships between feeding behaviour and the circulating levels of growth hormone in ruminants is discussed. 6. A method for the radioimmunoassay of ovine growth hormone is described and validated."}
{"id": "PMID:469782", "title": "Effect of acetylcholine stimulation on calcium and sodium uptake by the isolated rat pancreas.", "content": "1. The role of Ca2+ and Na+ ions in 'stimulus-secretion' coupling in the isolated uncinate pancreas of 4-week-old rats has been examined using radioisotope tracer techniques. The amount of 45Ca2+ and 22Na+ (mumole/g wet wt.) taken up by unstimulated glands was compared to that of glands in which amylase release was stimulated by ACh(10(-5) M) at various incubation times. 2. The amount of 45Ca taken up by the glands within 60 min of incubation was not found to be increased by the presence of ACh(10(-5) M). In fact, during short incubation periods the 45Ca uptake was significantly less in the stimulated glands than the unstimulated glands. 3. Presence of ACh(10(-5) M) did cause a significant initial increase in 22Na uptake lasting up to 20 min from onset of stimulation. 4. These results indicate that the rise in intracellular Ca2+ concentration which is suggested by an increase in 45Ca efflux during the action of pancreatic secretagogues, is not a consequence of increased 45Ca uptake by the pancreas; but they indicate that an initial action of ACh could be to elicit an increase in Na+ influx and that Na+ ions are likely to be involved in the action of ACh on pancreatic acinar cells.", "contents": "Effect of acetylcholine stimulation on calcium and sodium uptake by the isolated rat pancreas. 1. The role of Ca2+ and Na+ ions in 'stimulus-secretion' coupling in the isolated uncinate pancreas of 4-week-old rats has been examined using radioisotope tracer techniques. The amount of 45Ca2+ and 22Na+ (mumole/g wet wt.) taken up by unstimulated glands was compared to that of glands in which amylase release was stimulated by ACh(10(-5) M) at various incubation times. 2. The amount of 45Ca taken up by the glands within 60 min of incubation was not found to be increased by the presence of ACh(10(-5) M). In fact, during short incubation periods the 45Ca uptake was significantly less in the stimulated glands than the unstimulated glands. 3. Presence of ACh(10(-5) M) did cause a significant initial increase in 22Na uptake lasting up to 20 min from onset of stimulation. 4. These results indicate that the rise in intracellular Ca2+ concentration which is suggested by an increase in 45Ca efflux during the action of pancreatic secretagogues, is not a consequence of increased 45Ca uptake by the pancreas; but they indicate that an initial action of ACh could be to elicit an increase in Na+ influx and that Na+ ions are likely to be involved in the action of ACh on pancreatic acinar cells."}
{"id": "PMID:469785", "title": "Phasic firing enhances vasopressin release from the rat neurohypophysis.", "content": "1. Isolated rat neural lobes were incubated in vitro and electrically stimulated to release vasopressin. The released vasopressin was assayed using a radioimmunoassay and there was a reasonably good correlation (r = 0.81) between results obtained with this assay and those obtained by bioassay with the rat blood pressure method.2. Regular stimulation at frequencies of 5, 10 and 20 Hz released progressively more vasopressin and the release could be blocked by addition of tetrodotoxin to the incubation medium.3. Stimulation with pulse patterns derived from tape recordings of phasically firing units in the supraoptic nucleus of dehydrated rats released more vasopressin than the same number of pulses regularly spaced in time. In the range 2-8 pulses/sec vasopressin release was related to the pulse frequency within the bursts (r = 0.90) and the number of short (< 100 msec) interpulse intervals (r = 0.92). Vasopressin released per pulse increased over the frequency range 3-6 pulses/sec, but above 6 pulses/sec vasopressin release per pulse tended to diminish.4. We conclude that phasic firing of vasopressin neurosecretory cells may enhance vasopressin release in vivo and that an important factor in determining release is the number of short interspike intervals.", "contents": "Phasic firing enhances vasopressin release from the rat neurohypophysis. 1. Isolated rat neural lobes were incubated in vitro and electrically stimulated to release vasopressin. The released vasopressin was assayed using a radioimmunoassay and there was a reasonably good correlation (r = 0.81) between results obtained with this assay and those obtained by bioassay with the rat blood pressure method.2. Regular stimulation at frequencies of 5, 10 and 20 Hz released progressively more vasopressin and the release could be blocked by addition of tetrodotoxin to the incubation medium.3. Stimulation with pulse patterns derived from tape recordings of phasically firing units in the supraoptic nucleus of dehydrated rats released more vasopressin than the same number of pulses regularly spaced in time. In the range 2-8 pulses/sec vasopressin release was related to the pulse frequency within the bursts (r = 0.90) and the number of short (< 100 msec) interpulse intervals (r = 0.92). Vasopressin released per pulse increased over the frequency range 3-6 pulses/sec, but above 6 pulses/sec vasopressin release per pulse tended to diminish.4. We conclude that phasic firing of vasopressin neurosecretory cells may enhance vasopressin release in vivo and that an important factor in determining release is the number of short interspike intervals."}
{"id": "PMID:469787", "title": "Electrophysiological identification of two types of fibres in rat extraocular muscles.", "content": "1. The synaptic potentials and electrical properties of rat inferior rectus muscles were examined in vitro. 2. In most fibres the spontaneous synaptic activity consisted of typical miniature end-plate potentials which had a normal distribution of amplitudes and rather uniform time courses. Suprathreshold and maximal nerve stimulation evoked unitary end-plate potentials (e.p.p.s). The synaptic activity of these fibres could be recorded only in the innervation zone of the muscle. These fibres were identified as being focally innervated. 3. Focally innervated fibres gave action potentials upon direct and indirect stimulation. They had an effective resistance (Reff)  of 1.62 +/- 0.22 M omega (mean +/- S.E., twenty-two fibres) and a time constant (tau m) of 3.8 +/- 0.4 msec (twenty-one fibres). Voltage-current curves in control saline were linear between membrane potentials of -50 to -140 mV. 4. In a small number of fibres the spontaneous synaptic activity consisted of miniature small-nerve junction potentials which had a skewed distribution of amplitudes with predominance of smaller voltages and time courses with a wide range of variation. Nerve stimulation evoked composite small-nerve junction potentials (s.j.p.s) which could be resolved into unitary components by varying the strength of stimulation. S.j.p.s had a higher threshold than e.p.p.s. Synaptic potentials could be recorded outside the innervation zone, at various sites along the muscle length. These fibres were recognized as being multiply innervated with polyneuronal innervation. 5. Multiply innervated fibres lacked action potentials had a large Reff of 6.0 +/- 1.1 M omega (six fibres) and a prolonged tau m of 29.8 +/- 4.8 msec. Reff show a moderate decrease to hyperpolarization and a rather large decrease to depolarization which denote, respectively, the presence of anomalous and delayed reactification. 6. It is concluded that rat extraocular muscles contain at least two populations of muscle fibres that in terms of synaptic activity and electrical properties are comparable to twitch fibres of other mammalian muscles and to slow or tonic fibres of amphibians.", "contents": "Electrophysiological identification of two types of fibres in rat extraocular muscles. 1. The synaptic potentials and electrical properties of rat inferior rectus muscles were examined in vitro. 2. In most fibres the spontaneous synaptic activity consisted of typical miniature end-plate potentials which had a normal distribution of amplitudes and rather uniform time courses. Suprathreshold and maximal nerve stimulation evoked unitary end-plate potentials (e.p.p.s). The synaptic activity of these fibres could be recorded only in the innervation zone of the muscle. These fibres were identified as being focally innervated. 3. Focally innervated fibres gave action potentials upon direct and indirect stimulation. They had an effective resistance (Reff)  of 1.62 +/- 0.22 M omega (mean +/- S.E., twenty-two fibres) and a time constant (tau m) of 3.8 +/- 0.4 msec (twenty-one fibres). Voltage-current curves in control saline were linear between membrane potentials of -50 to -140 mV. 4. In a small number of fibres the spontaneous synaptic activity consisted of miniature small-nerve junction potentials which had a skewed distribution of amplitudes with predominance of smaller voltages and time courses with a wide range of variation. Nerve stimulation evoked composite small-nerve junction potentials (s.j.p.s) which could be resolved into unitary components by varying the strength of stimulation. S.j.p.s had a higher threshold than e.p.p.s. Synaptic potentials could be recorded outside the innervation zone, at various sites along the muscle length. These fibres were recognized as being multiply innervated with polyneuronal innervation. 5. Multiply innervated fibres lacked action potentials had a large Reff of 6.0 +/- 1.1 M omega (six fibres) and a prolonged tau m of 29.8 +/- 4.8 msec. Reff show a moderate decrease to hyperpolarization and a rather large decrease to depolarization which denote, respectively, the presence of anomalous and delayed reactification. 6. It is concluded that rat extraocular muscles contain at least two populations of muscle fibres that in terms of synaptic activity and electrical properties are comparable to twitch fibres of other mammalian muscles and to slow or tonic fibres of amphibians."}
{"id": "PMID:469794", "title": "Calcium-dependent potentials in the mammalian sympathetic neurone.", "content": "1. Intracellular recordings from post-ganglionic neurones of the rat superior cervical ganglion revealed two non-synaptic potentials dependent upon Ca2+, a hyperpolarizing afterpotential (h.a.p.) and a tetrodotoxin (TTX)-insensitive spike. 2. The h.a.p. followed regeneration discharge of the membrane potential in normal and TTX-containing Locke solution. 3. The h.a.p. appeared to arise from an increased K+ conductance because it was associated with a decrease in input resistance, reversed at -90 mV, and was proportional in magnitude to the extracellular K+ concentration. 4. Tetraethylammonium (TEA) and 4-aminopyridine (4-AP) apparently antagonized a voltage-sensitive K+ conductance because they broadened the action potential. However, these substances reduced only slightly the peak amplitude and earliest phases of the h.a.p. 5. The TTX-insensitive spike was most apparent when TEA was present and was invariably followed by an h.a.p. with a magnitude proportional to that of the spike. 6. The magnitude of the h.a.p. and the TTX-insensitive spike was directly proportional to the external Ca2+ concentration and was antagonized by Co2+ and Mn2+ in a dose-dependent fashion. 7. In normal Locke solution, Ba2+ antagonized the h.a.p. and allowed the neurone to sustain discharge during prolonged depolarization. In Locke solution containing TTX and TEA, Ba2+ reduced the magnitude of the h.a.p. but greatly increased the duration of the TTX-insensitive spike. 8. The h.a.p. was not significantly affected by altering external Cl- concentration and the TTX-insensitive spike was not reduced by altering external Na+ concentration. 9. It is concluded that the post-ganglionic neurone supports a regenerative Ca2+ conductance mechanism which in turn triggers an increased K+ conductance. The h.a.p. appears to result from outward K+ current in both a Ca2+ and voltage-dependent fashion.", "contents": "Calcium-dependent potentials in the mammalian sympathetic neurone. 1. Intracellular recordings from post-ganglionic neurones of the rat superior cervical ganglion revealed two non-synaptic potentials dependent upon Ca2+, a hyperpolarizing afterpotential (h.a.p.) and a tetrodotoxin (TTX)-insensitive spike. 2. The h.a.p. followed regeneration discharge of the membrane potential in normal and TTX-containing Locke solution. 3. The h.a.p. appeared to arise from an increased K+ conductance because it was associated with a decrease in input resistance, reversed at -90 mV, and was proportional in magnitude to the extracellular K+ concentration. 4. Tetraethylammonium (TEA) and 4-aminopyridine (4-AP) apparently antagonized a voltage-sensitive K+ conductance because they broadened the action potential. However, these substances reduced only slightly the peak amplitude and earliest phases of the h.a.p. 5. The TTX-insensitive spike was most apparent when TEA was present and was invariably followed by an h.a.p. with a magnitude proportional to that of the spike. 6. The magnitude of the h.a.p. and the TTX-insensitive spike was directly proportional to the external Ca2+ concentration and was antagonized by Co2+ and Mn2+ in a dose-dependent fashion. 7. In normal Locke solution, Ba2+ antagonized the h.a.p. and allowed the neurone to sustain discharge during prolonged depolarization. In Locke solution containing TTX and TEA, Ba2+ reduced the magnitude of the h.a.p. but greatly increased the duration of the TTX-insensitive spike. 8. The h.a.p. was not significantly affected by altering external Cl- concentration and the TTX-insensitive spike was not reduced by altering external Na+ concentration. 9. It is concluded that the post-ganglionic neurone supports a regenerative Ca2+ conductance mechanism which in turn triggers an increased K+ conductance. The h.a.p. appears to result from outward K+ current in both a Ca2+ and voltage-dependent fashion."}
{"id": "PMID:469795", "title": "Non-parallel enzyme secretion from rat pancreas in vitro studies.", "content": "1. The relative variations of rat pancreatic amylase versus lipase and chymotrypsinogen secretions have been studied in vitro with the help of two different techniques: in situ organ perfusion and incubation of pancreatic lobules. 2. In experiments on the perfused pancreas, with 8 C u.kg-1 hr-1 secretin added to the perfusion fluid, cholecystokinin-pancreozymin (8 ID u.kg-1) or pilocarpine (15 mg kg-1) both resulted in a significant change in the enzyme proportions in the juice. 3. In experiments on pancreatic lobules, the addition to the incubation medium of secretin (10(-7) M), alone or associated with cholecystokinin-pancreozymin (8 x 10(-7) M) or pilocarpine (10(-4) M) did not induce any change in the enzyme proportions in secretion. 4. It was concluded that the non-parallelism between enzyme secretions can occur in the rat upon pancreozyminic or cholinergic stimulation in vitro as well as in vivo (Dagorn, 1978) provided basal protein output is low enough. This is not the case when the tissular integrity of the pancreas is lost, such as in experiments on lobules. 5. This work confirms that pancreatic secretion derives from two intrapancreatic pools of different enzymatic composition, and gives a possible explanation for some discrepancies from the literature on the existence of non-parallel secretion.", "contents": "Non-parallel enzyme secretion from rat pancreas in vitro studies. 1. The relative variations of rat pancreatic amylase versus lipase and chymotrypsinogen secretions have been studied in vitro with the help of two different techniques: in situ organ perfusion and incubation of pancreatic lobules. 2. In experiments on the perfused pancreas, with 8 C u.kg-1 hr-1 secretin added to the perfusion fluid, cholecystokinin-pancreozymin (8 ID u.kg-1) or pilocarpine (15 mg kg-1) both resulted in a significant change in the enzyme proportions in the juice. 3. In experiments on pancreatic lobules, the addition to the incubation medium of secretin (10(-7) M), alone or associated with cholecystokinin-pancreozymin (8 x 10(-7) M) or pilocarpine (10(-4) M) did not induce any change in the enzyme proportions in secretion. 4. It was concluded that the non-parallelism between enzyme secretions can occur in the rat upon pancreozyminic or cholinergic stimulation in vitro as well as in vivo (Dagorn, 1978) provided basal protein output is low enough. This is not the case when the tissular integrity of the pancreas is lost, such as in experiments on lobules. 5. This work confirms that pancreatic secretion derives from two intrapancreatic pools of different enzymatic composition, and gives a possible explanation for some discrepancies from the literature on the existence of non-parallel secretion."}
{"id": "PMID:469798", "title": "Potassium activity in photoreceptors, glial cells and extracellular space in the drone retina: changes during photostimulation.", "content": "1. A double-barrelled potassium-sensitive micro-electrode was developed that was fine enough to record intracellular electrical potentials and potassium activities (aK) in the drone retina. 2. aK was measured in the photoreceptor cells, in the pigment (glial) cells, and in the extracellular space, in the superfused, cut, retina. The effect of photostimulation was studied: 20 msec light flashes, intense enough to evoke receptor potentials of maximum amplitude were presented, 1/sec, in a train lasting about 2 min. 3. In photoreceptors with membrane potentials greater than or equal to 50 mV aK in the dark was 79 mM, S.D. = 27 mM, n = 11. During photostimulation aK fell by 21.5 +/- 9.5 mM with a half-time of 30 +/- 22 sec. (A tentative conversion from activities to free concentrations can be made by taking the activity coefficient as 0.70 its value in the Ringer solution). 4. In pigment cells with membrane potentials greater than or equal to 50 mV, aK in the dark was 52  mM, S.D. = 13 mM, n = 11. During photostimulation aK increased by 14 +/- 5 mM. 5. In the extracellular space aK increased during photostimulation with a mean half-time of less than 1.3 sec to a maximum (mean value 14 mM, S.D. = 8.4 mM, n = 22), and then fell to a plateau. 6. It is estimated from the anatomy that the photoreceptors occupy approximately 38% of the total volume of the retina, the pigment cells 57%, and extracellular space 5%. Hence, it seems possible that during photostimulation nearly all the net loss of potassium from the photoreceptors is temporarily stored in the pigment cells. 7. Recordings were made in the extracellular space of the intact animal by passing the electrode through a hole in the cornea. The mean aK in the dark was 7.7 mM, S.E. = 0.4 mM, n = 22. In the superfused retina, aK in the dark was 6.3 mM, S.E. = 0.7 mM, n = 22, even though aK in the Ringer solution was 2.2 mM. Increasing the aK of the Ringer solution to 7.0 mM had no apparent effect on aK in the extracellular space at depths greater than 20 micron. 8. In the intact animal the amplitude and time course of the change in extracellular aK evoked by the standard pattern of photostimulation were within the range observed in the superfused preparation.", "contents": "Potassium activity in photoreceptors, glial cells and extracellular space in the drone retina: changes during photostimulation. 1. A double-barrelled potassium-sensitive micro-electrode was developed that was fine enough to record intracellular electrical potentials and potassium activities (aK) in the drone retina. 2. aK was measured in the photoreceptor cells, in the pigment (glial) cells, and in the extracellular space, in the superfused, cut, retina. The effect of photostimulation was studied: 20 msec light flashes, intense enough to evoke receptor potentials of maximum amplitude were presented, 1/sec, in a train lasting about 2 min. 3. In photoreceptors with membrane potentials greater than or equal to 50 mV aK in the dark was 79 mM, S.D. = 27 mM, n = 11. During photostimulation aK fell by 21.5 +/- 9.5 mM with a half-time of 30 +/- 22 sec. (A tentative conversion from activities to free concentrations can be made by taking the activity coefficient as 0.70 its value in the Ringer solution). 4. In pigment cells with membrane potentials greater than or equal to 50 mV, aK in the dark was 52  mM, S.D. = 13 mM, n = 11. During photostimulation aK increased by 14 +/- 5 mM. 5. In the extracellular space aK increased during photostimulation with a mean half-time of less than 1.3 sec to a maximum (mean value 14 mM, S.D. = 8.4 mM, n = 22), and then fell to a plateau. 6. It is estimated from the anatomy that the photoreceptors occupy approximately 38% of the total volume of the retina, the pigment cells 57%, and extracellular space 5%. Hence, it seems possible that during photostimulation nearly all the net loss of potassium from the photoreceptors is temporarily stored in the pigment cells. 7. Recordings were made in the extracellular space of the intact animal by passing the electrode through a hole in the cornea. The mean aK in the dark was 7.7 mM, S.E. = 0.4 mM, n = 22. In the superfused retina, aK in the dark was 6.3 mM, S.E. = 0.7 mM, n = 22, even though aK in the Ringer solution was 2.2 mM. Increasing the aK of the Ringer solution to 7.0 mM had no apparent effect on aK in the extracellular space at depths greater than 20 micron. 8. In the intact animal the amplitude and time course of the change in extracellular aK evoked by the standard pattern of photostimulation were within the range observed in the superfused preparation."}
{"id": "PMID:469799", "title": "Desensitization of glutamate receptors on innervated and denervated locust muscle fibres.", "content": "Depolarizations to L-glutamate, applied locally by microinophoresis to the extrajunctional membrane of locust extensor tibiae muscle fibres and measured either in current clamp or voltage clamp, increased in amplitude for equivalent doses of glutamate following chronic denervation of the muscle. 2. A two-pulse method was used to examine recovery from desentization of junctional and extrajunctional receptors. A 'response ratio', i.e. the amplitude of response to the second (test) of a pair of glutamate pulses over to the pulses. The 'response ratio' for extrajunctional depolarizations of innervated fibres increased exponentially with pulse interval, with a time constant of 15.6 +/- 4.7 sec (n = 11). Recovery of extrajunctional receptor populations from desensitization was accelerated after denervation. The recovery kinetics for responses from fibres 6-22 days after denervation were generally described by two exponential terms, with time constants in the range 0.5-10 sec which were inversely related to the glutamate sensitivity of the extrajunctional membrane. For junctional receptors on both innervated and denervated fibres the recovery kinetics were described by a single exponential with a time constant of 0.2-1 sec. 3. The results suggest that the increased extrajunctional glutamate sensitivity which occurs after denervation results from the 'appearance' of glutamate receptors with properties similar to those found at the post-junctional membrane on locust muscle fibres.", "contents": "Desensitization of glutamate receptors on innervated and denervated locust muscle fibres. Depolarizations to L-glutamate, applied locally by microinophoresis to the extrajunctional membrane of locust extensor tibiae muscle fibres and measured either in current clamp or voltage clamp, increased in amplitude for equivalent doses of glutamate following chronic denervation of the muscle. 2. A two-pulse method was used to examine recovery from desentization of junctional and extrajunctional receptors. A 'response ratio', i.e. the amplitude of response to the second (test) of a pair of glutamate pulses over to the pulses. The 'response ratio' for extrajunctional depolarizations of innervated fibres increased exponentially with pulse interval, with a time constant of 15.6 +/- 4.7 sec (n = 11). Recovery of extrajunctional receptor populations from desensitization was accelerated after denervation. The recovery kinetics for responses from fibres 6-22 days after denervation were generally described by two exponential terms, with time constants in the range 0.5-10 sec which were inversely related to the glutamate sensitivity of the extrajunctional membrane. For junctional receptors on both innervated and denervated fibres the recovery kinetics were described by a single exponential with a time constant of 0.2-1 sec. 3. The results suggest that the increased extrajunctional glutamate sensitivity which occurs after denervation results from the 'appearance' of glutamate receptors with properties similar to those found at the post-junctional membrane on locust muscle fibres."}
{"id": "PMID:469800", "title": "The importance of flow pulsatility for the rate of transvascular fluid filtration in lungs.", "content": "1. The rate of transvascular fluid filtration has been studied with a gravimetric technique in isolated perfused rabbit lungs during periods of elevated left atrial pressure (PLA). 2. Fluid filtration was expressed as the filtration coefficient, Kf (g/min x 100 g bloodless lung x mmHg PLA) and determined during alternately pulsatile and non-pulsatile perfusion in six zone III and three zone II/I lung preparations. Perfusion pattern was changed without interruption of flow. Mean in- and outflow pressures were kept constant. 3. In all the lungs it was found that Kf was higher during pulsatile than during non-pulsatile flow (P less than 0.01). Mean Kf (+/- S.E. of mean) for the zone III preparations was 0.42 (+/- 0.089) and 0.27 (+/- 0.057) for pulsatile and non-pulsatile perfusion, respectively. The corresponding figures for the zone II/I preparations were 0.11 (+/- 0.035) and 0.04 (+/- 0.030). 4. We suggest that the difference is due to a larger filtration area and/or a higher mean microvascular hydrostatic pressure during pulsatile than during non-pulsatile flow and not to a rise in hydraulic conductivity due to pressure pulsations ('stretched pores'). 5. When the water-exchange function of the lung is considered, flow pattern should be taken into account as an entity in its own right in addition to the steady state or the mean component of blood flow.", "contents": "The importance of flow pulsatility for the rate of transvascular fluid filtration in lungs. 1. The rate of transvascular fluid filtration has been studied with a gravimetric technique in isolated perfused rabbit lungs during periods of elevated left atrial pressure (PLA). 2. Fluid filtration was expressed as the filtration coefficient, Kf (g/min x 100 g bloodless lung x mmHg PLA) and determined during alternately pulsatile and non-pulsatile perfusion in six zone III and three zone II/I lung preparations. Perfusion pattern was changed without interruption of flow. Mean in- and outflow pressures were kept constant. 3. In all the lungs it was found that Kf was higher during pulsatile than during non-pulsatile flow (P less than 0.01). Mean Kf (+/- S.E. of mean) for the zone III preparations was 0.42 (+/- 0.089) and 0.27 (+/- 0.057) for pulsatile and non-pulsatile perfusion, respectively. The corresponding figures for the zone II/I preparations were 0.11 (+/- 0.035) and 0.04 (+/- 0.030). 4. We suggest that the difference is due to a larger filtration area and/or a higher mean microvascular hydrostatic pressure during pulsatile than during non-pulsatile flow and not to a rise in hydraulic conductivity due to pressure pulsations ('stretched pores'). 5. When the water-exchange function of the lung is considered, flow pattern should be taken into account as an entity in its own right in addition to the steady state or the mean component of blood flow."}
{"id": "PMID:469801", "title": "The secretion of calcium and phosphorus into milk.", "content": "1. The time course of appearance of radioactivity in milk was studied following close-arterial infusion of labelled phosphate, Ca or leucine into the mammary artery of lactating goats. Maximum activities were reached at 1.5 hr in all milk fractions including inorganic soluble phosphate, inorganic colloidal phosphate, casein P, soluble Ca, protein-associated Ca and casein. 2. At 0.5 hr, labelling of the soluble and colloidal phosphate fractions was significantly higher than that of the casein P. 3. Recovery of 32P or 47Ca 3 or more hours after infusion into the cistern of the mammary glands was 98% or greater, indicating that the mammary epithelium is virtually impermeable to [32P]phosphate and 47Ca in the milk to blood direction. 4. Ca and P failed to enter milk in excess of the normal secretion rate when the milk was diluted with isosmotic sucrose given by intraductal injection. 5. These data suggest that milk Ca and phosphate in their various forms are secreted, like protein and lactose, by exocytosis of Golgi vesicles. Unless a paracellular pathway is present, as in oxytocin-treated animals, the milk concentrations are maintained by virtue of the impermeability of the mammary epithelium to these substances.", "contents": "The secretion of calcium and phosphorus into milk. 1. The time course of appearance of radioactivity in milk was studied following close-arterial infusion of labelled phosphate, Ca or leucine into the mammary artery of lactating goats. Maximum activities were reached at 1.5 hr in all milk fractions including inorganic soluble phosphate, inorganic colloidal phosphate, casein P, soluble Ca, protein-associated Ca and casein. 2. At 0.5 hr, labelling of the soluble and colloidal phosphate fractions was significantly higher than that of the casein P. 3. Recovery of 32P or 47Ca 3 or more hours after infusion into the cistern of the mammary glands was 98% or greater, indicating that the mammary epithelium is virtually impermeable to [32P]phosphate and 47Ca in the milk to blood direction. 4. Ca and P failed to enter milk in excess of the normal secretion rate when the milk was diluted with isosmotic sucrose given by intraductal injection. 5. These data suggest that milk Ca and phosphate in their various forms are secreted, like protein and lactose, by exocytosis of Golgi vesicles. Unless a paracellular pathway is present, as in oxytocin-treated animals, the milk concentrations are maintained by virtue of the impermeability of the mammary epithelium to these substances."}
{"id": "PMID:469803", "title": "Fever induced in rabbits by intravenous injection of bovine serum albumin.", "content": "1. Bovine serum albumin injected into conscious rabbits produced a dose-dependent fever of short latency and duration of at least 2 1/2 hr. 2. The fever was not due to contamination by other proteins nor due to contamination by bacterial endotoxin. 3. The fever following albumin injection had characteristics similar to endogenous pyrogen fever. 4. Some traditional ways of distinguishing bacterial endotoxin fever from other types of fever need to be reassessed.", "contents": "Fever induced in rabbits by intravenous injection of bovine serum albumin. 1. Bovine serum albumin injected into conscious rabbits produced a dose-dependent fever of short latency and duration of at least 2 1/2 hr. 2. The fever was not due to contamination by other proteins nor due to contamination by bacterial endotoxin. 3. The fever following albumin injection had characteristics similar to endogenous pyrogen fever. 4. Some traditional ways of distinguishing bacterial endotoxin fever from other types of fever need to be reassessed."}
{"id": "PMID:469805", "title": "Electrophysiological and sensory properties of the thalamic reticular neurones related to somatic sensation in rats.", "content": "1. In urethane-anaesthetized rats, studies were made on unit discharges of neurones of the thalamic reticular nucleus (tr) receiving the somatosensory input. Such units were found distributed in a part of the tr (s-tr) surrounding the anterior and anterolateral borders of the ventrobasal nuclear complex (vb). 2. A series of five to ten bursts of grouped discharges were elicited in the s-tr neurones as orthodromic responses to single shock stimulation of the medial lemniscus (ml) or the somatosensory cortex (smc). In contrast to this, the vb relay cells were fired by ml impulses only once at short latencies, though later there occurred a series of two to three bursts of grouped discharges. The same response pattern was seen by stimulating the smc, except that the initial response to smc shocks was of antidromic origin. 3. The mean response latency of the s-tr neurones to ml shocks was about 0.6 msec longer than that of the vb relay cells. Ml fibres connected to the s-tr neurones had the same distribution of conduction velocity as those connected to the vb relay cells. It is suggested that most, if not all, of the s-tr neurones are innervated by axon collaterals of the vb relay cells. 4. About half the s-tr units were activated from vibrissae. For most of the remaining half either movements of hairs or touch to the skin were effective. In the vb, the units discharged by movements of hairs were most numerous and those activated from vibrissae were less frequent. While responses of the s-tr neurones to adequate stimuli were almost exclusively of phasic type, 30% of the responses of the vb relay cells were tonic and the remainder were phasic. 5. By exploring the tr extensively, the s-tr was found to be contiguous caudally to the perigeniculate reticular nucleus which is immediately adjacent to the dorsal lateral geniculate nucleus and contains reticular neurones activated by visual stimuli. Though few in number, units were found in the tr which received a convergence of somatosensory and visual inputs or of somatosensory and auditory inputs.", "contents": "Electrophysiological and sensory properties of the thalamic reticular neurones related to somatic sensation in rats. 1. In urethane-anaesthetized rats, studies were made on unit discharges of neurones of the thalamic reticular nucleus (tr) receiving the somatosensory input. Such units were found distributed in a part of the tr (s-tr) surrounding the anterior and anterolateral borders of the ventrobasal nuclear complex (vb). 2. A series of five to ten bursts of grouped discharges were elicited in the s-tr neurones as orthodromic responses to single shock stimulation of the medial lemniscus (ml) or the somatosensory cortex (smc). In contrast to this, the vb relay cells were fired by ml impulses only once at short latencies, though later there occurred a series of two to three bursts of grouped discharges. The same response pattern was seen by stimulating the smc, except that the initial response to smc shocks was of antidromic origin. 3. The mean response latency of the s-tr neurones to ml shocks was about 0.6 msec longer than that of the vb relay cells. Ml fibres connected to the s-tr neurones had the same distribution of conduction velocity as those connected to the vb relay cells. It is suggested that most, if not all, of the s-tr neurones are innervated by axon collaterals of the vb relay cells. 4. About half the s-tr units were activated from vibrissae. For most of the remaining half either movements of hairs or touch to the skin were effective. In the vb, the units discharged by movements of hairs were most numerous and those activated from vibrissae were less frequent. While responses of the s-tr neurones to adequate stimuli were almost exclusively of phasic type, 30% of the responses of the vb relay cells were tonic and the remainder were phasic. 5. By exploring the tr extensively, the s-tr was found to be contiguous caudally to the perigeniculate reticular nucleus which is immediately adjacent to the dorsal lateral geniculate nucleus and contains reticular neurones activated by visual stimuli. Though few in number, units were found in the tr which received a convergence of somatosensory and visual inputs or of somatosensory and auditory inputs."}
{"id": "PMID:469809", "title": "Distribution of climbing fibres on cerebellar Purkinje cells in X-irradiated rats. An electrophysiological study.", "content": "1. The distribution of climbing fibres on cerebellar Purkinje cells has been studied with intracellular recordings in X-irradiated and normal rats. 2. In the treated rats, multiple steps in the post-synaptic potential were elicited in 57% of the Purkinje cells by graded stimulation of the climbing fibres, the response was all-or-none in character in the other cells and in all Purkinje cells recorded in normal animals. In the neurones exhibiting the former type of response, no collision was seen along the afferent fibres during interaction experiments between just-threshold juxtafastigial and maximal olivary stimulations, whereas a collision always occurred when all-or-none responses were recorded. 3. These results show that in X-irradiated rats, the majority of Purkinje cells have a multiple innervation by two to four climbing fibres, instead of the one-to-one relationship seen normally. 4. Input resistances and total electrotonic lengths of Purkinje cells were measured in normal and treated rats. Mean values for these two parameters were higher than normal in multiply innervated cells. 5. Mean time course and mean current for reversal of the post-synaptic potential elicited in Purkinje cells by stimulation of the climbing fibres were nearly the same in mono- and in multiply innervated neurones. In multiply innervated cells, time courses and currents for reversal were independent of the size of the response or varied slightly with it, suggesting that the climbing fibres involved innervated territories whose electrotonic distance from the recording site were either the same or slightly different. 6. Interactions between two all-or-none steps of the graded post-synaptic potential evoked in multiply innervated cells by juxtafastigial and olivary stimulations revealed either a very weak or a very marked shunting effect between synapses of the two climbing fibres involved. 7. These results indicate that the over-all distribution of climbing fibre synapses on multiply innervated Purkinje cells is not grossly abnormal and that two fibres contacting a given cell can be either intermingled on the same dendrites, or segregated on distinct dendritic branches. 8. In general, the present study does not suggest the existence of a strong competition among climbing fibres innervating each Purkinje cell during development at least when granule cells are absent.", "contents": "Distribution of climbing fibres on cerebellar Purkinje cells in X-irradiated rats. An electrophysiological study. 1. The distribution of climbing fibres on cerebellar Purkinje cells has been studied with intracellular recordings in X-irradiated and normal rats. 2. In the treated rats, multiple steps in the post-synaptic potential were elicited in 57% of the Purkinje cells by graded stimulation of the climbing fibres, the response was all-or-none in character in the other cells and in all Purkinje cells recorded in normal animals. In the neurones exhibiting the former type of response, no collision was seen along the afferent fibres during interaction experiments between just-threshold juxtafastigial and maximal olivary stimulations, whereas a collision always occurred when all-or-none responses were recorded. 3. These results show that in X-irradiated rats, the majority of Purkinje cells have a multiple innervation by two to four climbing fibres, instead of the one-to-one relationship seen normally. 4. Input resistances and total electrotonic lengths of Purkinje cells were measured in normal and treated rats. Mean values for these two parameters were higher than normal in multiply innervated cells. 5. Mean time course and mean current for reversal of the post-synaptic potential elicited in Purkinje cells by stimulation of the climbing fibres were nearly the same in mono- and in multiply innervated neurones. In multiply innervated cells, time courses and currents for reversal were independent of the size of the response or varied slightly with it, suggesting that the climbing fibres involved innervated territories whose electrotonic distance from the recording site were either the same or slightly different. 6. Interactions between two all-or-none steps of the graded post-synaptic potential evoked in multiply innervated cells by juxtafastigial and olivary stimulations revealed either a very weak or a very marked shunting effect between synapses of the two climbing fibres involved. 7. These results indicate that the over-all distribution of climbing fibre synapses on multiply innervated Purkinje cells is not grossly abnormal and that two fibres contacting a given cell can be either intermingled on the same dendrites, or segregated on distinct dendritic branches. 8. In general, the present study does not suggest the existence of a strong competition among climbing fibres innervating each Purkinje cell during development at least when granule cells are absent."}
{"id": "PMID:469811", "title": "Immunological detection of somatostatin in a primitive hypothalamic mouse cell line, precursor of a neurophysin cell lineage.", "content": "A primitive nerve cell line (F7 clone), obtained by SV 40 transformation of mouse fetal hypothalamic cells, has been previously shown to share some properties with a progenitor cell for neurophysin synthesizing neuron. This paper describes the immunological detection, at the light microscope level, of somatostatin in this cell line. A SV 40 transformed neurosecretory clone, synthesizing neurophysin and vasopressin, was used as control.", "contents": "Immunological detection of somatostatin in a primitive hypothalamic mouse cell line, precursor of a neurophysin cell lineage. A primitive nerve cell line (F7 clone), obtained by SV 40 transformation of mouse fetal hypothalamic cells, has been previously shown to share some properties with a progenitor cell for neurophysin synthesizing neuron. This paper describes the immunological detection, at the light microscope level, of somatostatin in this cell line. A SV 40 transformed neurosecretory clone, synthesizing neurophysin and vasopressin, was used as control."}
{"id": "PMID:469812", "title": "Hypothalamic neurones in culture. II. A progress report.", "content": "Cultures of the hypothalamic supraoptic nucleus area were prepared from 1--10 day old rats by means of the roller-tube technique. After 3 weeks in vitro large nerve cells with long, thin and repeatedly branching axons could be observed. In silver-stained co-cultures of hypothalamic and hypophysial tissue, fibers were seen to bridge the gap between the adjacent explants. Intracellular current injection into the large nerve cells elicited single or repetitive action potentials. Two types of spontaneous discharge patterns were observed. The majority of cells fired randomly and the remainder displayed a phasic dicharge pattern. Simultaneous recordings from 2 cells disclosed that pairs of phasic cells had a tendency to fire in synchronous bursts.", "contents": "Hypothalamic neurones in culture. II. A progress report. Cultures of the hypothalamic supraoptic nucleus area were prepared from 1--10 day old rats by means of the roller-tube technique. After 3 weeks in vitro large nerve cells with long, thin and repeatedly branching axons could be observed. In silver-stained co-cultures of hypothalamic and hypophysial tissue, fibers were seen to bridge the gap between the adjacent explants. Intracellular current injection into the large nerve cells elicited single or repetitive action potentials. Two types of spontaneous discharge patterns were observed. The majority of cells fired randomly and the remainder displayed a phasic dicharge pattern. Simultaneous recordings from 2 cells disclosed that pairs of phasic cells had a tendency to fire in synchronous bursts."}
{"id": "PMID:469813", "title": "[Immunocytochemical study of the maturation of the hypothalamo-neuro-hypophysial axes in the human fetus (author's transl)].", "content": "1. The development of the hypothalamo-neurohypophysial system was studied using immunocytochemistry with various antisera : anti-neurophysin, anti-oxytocin, anti-vasopressin, anti-vasotocin and anti-somatostatin. 2. Immunocytochemical investigation shows that anti-vasopressin and anti-vasotocin sera react with both vasopressin and vasotocin, whereas the anti-oxytocin serum specifically reveals the oxytocin-containing structures (Fig. 1g, h, i). 3. Perikarya stained with anti-neurophysin, anti-vasopressin and anti-vasotocin sera can be seen from the 11th week of fetal life (Fig. 1a) first in the supra-optic nucleus (SON), then in the para-ventricular nucleus (PVN). Their axons reach the neural lobe as early as the 11th week (Fig. 1b, c). 4. Oxytocin-containing perikarya appear simultaneously in the PVN (Fig. 1e) and SON during the 13th week. 5. From the 16th week, neurons stained with the anti-somatostatin serum can be seen among the neurophysin-positive cell-bodies of the SON and PVN. 6. The various populations of magnocellular neurons show a significant increase in number, especially up to the 19th week, and an increase in their hormonal content up to birth.", "contents": "[Immunocytochemical study of the maturation of the hypothalamo-neuro-hypophysial axes in the human fetus (author's transl)]. 1. The development of the hypothalamo-neurohypophysial system was studied using immunocytochemistry with various antisera : anti-neurophysin, anti-oxytocin, anti-vasopressin, anti-vasotocin and anti-somatostatin. 2. Immunocytochemical investigation shows that anti-vasopressin and anti-vasotocin sera react with both vasopressin and vasotocin, whereas the anti-oxytocin serum specifically reveals the oxytocin-containing structures (Fig. 1g, h, i). 3. Perikarya stained with anti-neurophysin, anti-vasopressin and anti-vasotocin sera can be seen from the 11th week of fetal life (Fig. 1a) first in the supra-optic nucleus (SON), then in the para-ventricular nucleus (PVN). Their axons reach the neural lobe as early as the 11th week (Fig. 1b, c). 4. Oxytocin-containing perikarya appear simultaneously in the PVN (Fig. 1e) and SON during the 13th week. 5. From the 16th week, neurons stained with the anti-somatostatin serum can be seen among the neurophysin-positive cell-bodies of the SON and PVN. 6. The various populations of magnocellular neurons show a significant increase in number, especially up to the 19th week, and an increase in their hormonal content up to birth."}
{"id": "PMID:469828", "title": "Relationship between plasma and uterine oestradiol in pseudopregnant rabbits.", "content": "Plasma oestradiol concentrations did not change during hCG-induced pseudopregnancy except for a slight increase on Day 18. However, a marked decline was observed in tissue oestradiol on Day 3 whether expressed on the basis of tissue wet weight or protein. The lowest concentration was found at Day 6 but the Day 18 values were comparable to those of untreated controls.", "contents": "Relationship between plasma and uterine oestradiol in pseudopregnant rabbits. Plasma oestradiol concentrations did not change during hCG-induced pseudopregnancy except for a slight increase on Day 18. However, a marked decline was observed in tissue oestradiol on Day 3 whether expressed on the basis of tissue wet weight or protein. The lowest concentration was found at Day 6 but the Day 18 values were comparable to those of untreated controls."}
{"id": "PMID:469829", "title": "The concentration of carnitine in the luminal fluid of the testis and epididymis of the rat and some other mammals.", "content": "Luminal fluid was collected by micropuncture techniques from the testis and epididymis of the rat, hamster, rabbit, boar and ram and the concentration of free L-carnitine in the fluid was estimated using enzymic methods. Carnitine was present in the testicular fluid of the rat in concentrations less than 1 mM but increased down the epididymis to reach 53 mM in luminal fluid from the cauda epididymidis, approximately 2000 times higher than in blood plasma. A high concentration was first found in the luminal fluid from the distal caput epididymidis, at about the point where the spermatozoa become motile. Carnitine was also present in the epididymal luminal fluid of the other species studied; the amounts were not as high as those in the rat but were still higher than those in blood plasma.", "contents": "The concentration of carnitine in the luminal fluid of the testis and epididymis of the rat and some other mammals. Luminal fluid was collected by micropuncture techniques from the testis and epididymis of the rat, hamster, rabbit, boar and ram and the concentration of free L-carnitine in the fluid was estimated using enzymic methods. Carnitine was present in the testicular fluid of the rat in concentrations less than 1 mM but increased down the epididymis to reach 53 mM in luminal fluid from the cauda epididymidis, approximately 2000 times higher than in blood plasma. A high concentration was first found in the luminal fluid from the distal caput epididymidis, at about the point where the spermatozoa become motile. Carnitine was also present in the epididymal luminal fluid of the other species studied; the amounts were not as high as those in the rat but were still higher than those in blood plasma."}
{"id": "PMID:469830", "title": "Survival of frozen mouse embryos after rapid thawing from -196 degrees C.", "content": "The effect of the rate of rewarming on the survival of 8-cell mouse embryos and blastocysts was examined. The samples were slowly cooled (0.3--0.6 degrees C/min) in 1.5 M-DMSO to temperatures between -10 and -80 degrees C before direct transfer to liquid nitrogen (-196 degrees C). Embryos survived rapid thawing (275--500 degrees C/min) only when slow cooling was terminated at relatively high subzero temperatures (-10 to -50 degrees C). The highest levels of survival in vitro of rapidly thawed 8-cell embryos were obtained after transfer to -196 degrees C from -35 and -40 degrees C (72 to 88%) and of rapidly thawed blastocysts after transfer from -25 to -50 degrees C (69 to 74%). By contrast, for embryos to survive slow thawing (8 to 20 degrees C/min) slow cooling to lower subzero temperatures (-60 degrees C and below) was required before transfer to -196 degrees C. The results indicate that embryos transferred to -196 degrees C from high subzero temperatures contain sufficient intracellular ice to damage them during slow warming but to permit survival after rapid warming. Survival of embryos after rapid dilution of DMSO at room temperature was similar to that after slow (stepwise) dilution at 0 degrees C. There was no difference between the viability of rapidly and slowly thawed embryos after transfer to pseudopregnant foster mothers. It is concluded that the behaviour of mammalian embryos subjected to the stresses of freezing and thawing is similar to that of other mammalian cells. A simpler and quicker method for the preservation of mouse embryos is described.", "contents": "Survival of frozen mouse embryos after rapid thawing from -196 degrees C. The effect of the rate of rewarming on the survival of 8-cell mouse embryos and blastocysts was examined. The samples were slowly cooled (0.3--0.6 degrees C/min) in 1.5 M-DMSO to temperatures between -10 and -80 degrees C before direct transfer to liquid nitrogen (-196 degrees C). Embryos survived rapid thawing (275--500 degrees C/min) only when slow cooling was terminated at relatively high subzero temperatures (-10 to -50 degrees C). The highest levels of survival in vitro of rapidly thawed 8-cell embryos were obtained after transfer to -196 degrees C from -35 and -40 degrees C (72 to 88%) and of rapidly thawed blastocysts after transfer from -25 to -50 degrees C (69 to 74%). By contrast, for embryos to survive slow thawing (8 to 20 degrees C/min) slow cooling to lower subzero temperatures (-60 degrees C and below) was required before transfer to -196 degrees C. The results indicate that embryos transferred to -196 degrees C from high subzero temperatures contain sufficient intracellular ice to damage them during slow warming but to permit survival after rapid warming. Survival of embryos after rapid dilution of DMSO at room temperature was similar to that after slow (stepwise) dilution at 0 degrees C. There was no difference between the viability of rapidly and slowly thawed embryos after transfer to pseudopregnant foster mothers. It is concluded that the behaviour of mammalian embryos subjected to the stresses of freezing and thawing is similar to that of other mammalian cells. A simpler and quicker method for the preservation of mouse embryos is described."}
{"id": "PMID:469831", "title": "Influence of dose, repeated treatment and batch of hormone on ovarian response in heifers treated with PMSG.", "content": "The effect of two dose levels (1000 and 2000 i.u.) of three different commercially available batches of PMSG on the ovarian response (ovulations and follicles greater than 10 mn) of 42 heifers was examined in a randomized incomplete block experiment. Each animal was subjected to two consecutive but different treatments. A significant effect of dose was observed and there were fewer ovulations, but no reduction in the number of follicles, after the second PMSG treatment. There was no evidence that the ovarian response was affected by the PMSG batch used.", "contents": "Influence of dose, repeated treatment and batch of hormone on ovarian response in heifers treated with PMSG. The effect of two dose levels (1000 and 2000 i.u.) of three different commercially available batches of PMSG on the ovarian response (ovulations and follicles greater than 10 mn) of 42 heifers was examined in a randomized incomplete block experiment. Each animal was subjected to two consecutive but different treatments. A significant effect of dose was observed and there were fewer ovulations, but no reduction in the number of follicles, after the second PMSG treatment. There was no evidence that the ovarian response was affected by the PMSG batch used."}
{"id": "PMID:469832", "title": "Proteins in the uterine secretions of the cow.", "content": "Cows of normal reproductive history were treated with progesterone for periods of 2--3 months. The uterine secretions yielded 5 major fractions of macromolecular components. Two of the fractions comprised serum proteins. In the other 3 fractions at least 9 non-serum proteins were observed, 7 in one fraction, but in only small amounts. Of the remaining 2 non-serum proteins one is an acid phosphatase recoverable in small amounts, and the other is lactoferrin and is the major non-serum protein present in the uterine secretions of progesterone-treated cows.", "contents": "Proteins in the uterine secretions of the cow. Cows of normal reproductive history were treated with progesterone for periods of 2--3 months. The uterine secretions yielded 5 major fractions of macromolecular components. Two of the fractions comprised serum proteins. In the other 3 fractions at least 9 non-serum proteins were observed, 7 in one fraction, but in only small amounts. Of the remaining 2 non-serum proteins one is an acid phosphatase recoverable in small amounts, and the other is lactoferrin and is the major non-serum protein present in the uterine secretions of progesterone-treated cows."}
{"id": "PMID:469833", "title": "The role of the seminal vesicles, coagulating glands and prostate glands on the fertility and fecundity of mice.", "content": "Female mice were allowed to mate with males which had been sham-operated (Group 1); had seminal vesicles, coagulating glands and ventral and dorsal prostate glands removed (Group 2); had the seminal vesicles removed (Group 3); had the coagulating glands removed (Group 4) or had the ventral and dorsal prostate gland removed (Group 5). The pregnancy rate was normal in Groups 1 and 4, severely reduced in Groups 2 and 3 and less so in Group 5. Litter size was reduced in Groups 2 and 3 but not in Group 5. It is suggested that the seminal vesicles and possibly the prostate glands are important in the production of young in mice.", "contents": "The role of the seminal vesicles, coagulating glands and prostate glands on the fertility and fecundity of mice. Female mice were allowed to mate with males which had been sham-operated (Group 1); had seminal vesicles, coagulating glands and ventral and dorsal prostate glands removed (Group 2); had the seminal vesicles removed (Group 3); had the coagulating glands removed (Group 4) or had the ventral and dorsal prostate gland removed (Group 5). The pregnancy rate was normal in Groups 1 and 4, severely reduced in Groups 2 and 3 and less so in Group 5. Litter size was reduced in Groups 2 and 3 but not in Group 5. It is suggested that the seminal vesicles and possibly the prostate glands are important in the production of young in mice."}
{"id": "PMID:469834", "title": "Prostaglandin-induced luteolysis in pregnant and pseudopregnant rabbits and the resultant effects on the myometrial activity.", "content": "The effect of prostaglandin (PG)-induced luteolysis on the myometrial activity in 20--21-day-pregnant and 11--12-day-pseudopregnant rabbits was studied by intrauterine pressure (IUP) recording during PG infusions. The same dose of PG (10 micrograms/h during 8h) was also given to 7 non-pregnant (untreated) does that were used as controls. Peripheral plasma concentration of progesterone and oestradiol-17 beta were measured at 2-h intervals during the infusion. Plasma progesterone level decreased significantly within 2 h or the start of infusion in pregnant and pseudopregnant does and continued to decrease; at the end of 8 h, the concentrations were 31 and 41%, respectively, of the pre-infusion levels. The amplitude of uterine contractions increased significantly after 4 h in pseudopregnant does, increased slightly but insignificantly in the pregnant does and showed no significant change in the non-pregnant does during PG infusion. The amplitudes developed in the pregnant and pseudopregnant does were significantly different. The direct effect of progesterone (1--3 micrograms/h during 4 h) was also studied in 7 non-pregnant rabbits. After 2 h the amplitude of contractions had decreased markedly and the pattern of activity had become irregular. The results support the concept of a myometrial inhibitory factor other than progesterone in rabbit pregnancy and suggest that this factor(s) originates in conceptus.", "contents": "Prostaglandin-induced luteolysis in pregnant and pseudopregnant rabbits and the resultant effects on the myometrial activity. The effect of prostaglandin (PG)-induced luteolysis on the myometrial activity in 20--21-day-pregnant and 11--12-day-pseudopregnant rabbits was studied by intrauterine pressure (IUP) recording during PG infusions. The same dose of PG (10 micrograms/h during 8h) was also given to 7 non-pregnant (untreated) does that were used as controls. Peripheral plasma concentration of progesterone and oestradiol-17 beta were measured at 2-h intervals during the infusion. Plasma progesterone level decreased significantly within 2 h or the start of infusion in pregnant and pseudopregnant does and continued to decrease; at the end of 8 h, the concentrations were 31 and 41%, respectively, of the pre-infusion levels. The amplitude of uterine contractions increased significantly after 4 h in pseudopregnant does, increased slightly but insignificantly in the pregnant does and showed no significant change in the non-pregnant does during PG infusion. The amplitudes developed in the pregnant and pseudopregnant does were significantly different. The direct effect of progesterone (1--3 micrograms/h during 4 h) was also studied in 7 non-pregnant rabbits. After 2 h the amplitude of contractions had decreased markedly and the pattern of activity had become irregular. The results support the concept of a myometrial inhibitory factor other than progesterone in rabbit pregnancy and suggest that this factor(s) originates in conceptus."}
{"id": "PMID:469835", "title": "Effect of sodium nitrite on the alpha-chlorohydrin-induced lesion of the testis--epididymis complexin the rat.", "content": "Administration of vasodilator, sodium nitrite (20 mg/kg body weight), 30 min before alpha-chlorohydrin treatment (90 mg/kg body weight) prevented the chlorohydrin-induced lesion in rat testis--epididymis complex. However, administration of vasodilator 90 min after alpha-chlorohydrin treatment did not prevent the chlorohydrin-induced lesion in the testis--epididymis complex. These observations suggest that the testicular vasculature is involved in drug action.", "contents": "Effect of sodium nitrite on the alpha-chlorohydrin-induced lesion of the testis--epididymis complexin the rat. Administration of vasodilator, sodium nitrite (20 mg/kg body weight), 30 min before alpha-chlorohydrin treatment (90 mg/kg body weight) prevented the chlorohydrin-induced lesion in rat testis--epididymis complex. However, administration of vasodilator 90 min after alpha-chlorohydrin treatment did not prevent the chlorohydrin-induced lesion in the testis--epididymis complex. These observations suggest that the testicular vasculature is involved in drug action."}
{"id": "PMID:469836", "title": "A microsystem for the extraction and in-vitro translation of mouse embryo mRNA.", "content": "A micromethod is described for the extraction of mRNA from mouse ova and its translation in a message-dependent rabbit reticulocyte lysate system. Using this technique it has been possible, by SDS polyacrylamide gel fluorography, to visualize the products from the equivalent of 12.5 eggs.", "contents": "A microsystem for the extraction and in-vitro translation of mouse embryo mRNA. A micromethod is described for the extraction of mRNA from mouse ova and its translation in a message-dependent rabbit reticulocyte lysate system. Using this technique it has been possible, by SDS polyacrylamide gel fluorography, to visualize the products from the equivalent of 12.5 eggs."}
{"id": "PMID:469837", "title": "Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat.", "content": "Controlled perfusion through the lumen of the distal cauda epididymidis in the anaesthetized rat has been explored as a means of examining physiological exchanges from blood across the epididymal epithelium. The mean length of the perfused, sperm-free, tubule was 14.5 cm (+/- 1.5 s.e.m., n = 9). No cholesterol, protein or sialic acid was detected in the perfusate at flow rates exceeding 10 microliters/min, but at rates of 0.4--1.2 microliters/min, protein appeared at concentrations of 0.21--0.55 mg/ml (i.e. secretion rates of 0.21--0.83 micrograms/min; 3 rats). Glucose was detected at all perfusion rates (3--27 microliters/min) at concentrations of 0.06--0.58 mM (0.8--6.8% blood levels). During intravenous infusions of 3H2O, radioactivity in the perfusate rapidly attained 87% blood plasma concentrations; no radioactivity was detected when carboxy-E114C]dextran or methoxy-[3H]inulin were infused. Radioactivity appeared in the epididymal perfusate to 1--7% of blood levels during intravenous infusions of D-E1U-1RC]glucose or 3-O-methyl[1-3H]glucose. This evidence suggests that the preparation is physiological and could be used to explore the dynamics of exchanges between blood and epididymis.", "contents": "Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat. Controlled perfusion through the lumen of the distal cauda epididymidis in the anaesthetized rat has been explored as a means of examining physiological exchanges from blood across the epididymal epithelium. The mean length of the perfused, sperm-free, tubule was 14.5 cm (+/- 1.5 s.e.m., n = 9). No cholesterol, protein or sialic acid was detected in the perfusate at flow rates exceeding 10 microliters/min, but at rates of 0.4--1.2 microliters/min, protein appeared at concentrations of 0.21--0.55 mg/ml (i.e. secretion rates of 0.21--0.83 micrograms/min; 3 rats). Glucose was detected at all perfusion rates (3--27 microliters/min) at concentrations of 0.06--0.58 mM (0.8--6.8% blood levels). During intravenous infusions of 3H2O, radioactivity in the perfusate rapidly attained 87% blood plasma concentrations; no radioactivity was detected when carboxy-E114C]dextran or methoxy-[3H]inulin were infused. Radioactivity appeared in the epididymal perfusate to 1--7% of blood levels during intravenous infusions of D-E1U-1RC]glucose or 3-O-methyl[1-3H]glucose. This evidence suggests that the preparation is physiological and could be used to explore the dynamics of exchanges between blood and epididymis."}
{"id": "PMID:469838", "title": "Factors affecting the entry of testosterone into the lumen of the cauda epididymidis of the anaesthetized rat.", "content": "When [3H]testosterone was infused into the general circulation of the rat, perfusion of a length of the cauda epididymidis (17 +/- 1.0 (s.e.m.) cm, n = 36) with perfusates of varied composition revealed a low entry of radioactivity (1--10% plasma levels; 10 exps) with protein-free perfusates, and a greater entry (15--48%; 10 exps) when the perfusate contained bovine serum albumin (38 mg/ml). When the perfusate contained ovine or rat testicular fluid, or rat epididymal fluid at protein concentrations of 3 mg/ml or less, the entry of radioactivity into the epididymis was greater than when the perfusate contained 3 mg BSA/ml. The addition of ovine rete testis fluid protein (3 mg/ml to BSA (38 mg/ml) in the perfusate increased the uptake of radioactivity (58--106%; 6 exps). Radioactivity in blood was principally associated with testosterone (90, 95% total blood activity, 2 rats), whereas both [3H]testosterone (37, 41% total perfusate activity) and [3H]dihydrotestosterone (42, 63% total perfusate activity) was present in BSA-containing perfusates. The proportion of dihydrotestosterone appeared to increase when the perfusate contained protein of testicular origin.", "contents": "Factors affecting the entry of testosterone into the lumen of the cauda epididymidis of the anaesthetized rat. When [3H]testosterone was infused into the general circulation of the rat, perfusion of a length of the cauda epididymidis (17 +/- 1.0 (s.e.m.) cm, n = 36) with perfusates of varied composition revealed a low entry of radioactivity (1--10% plasma levels; 10 exps) with protein-free perfusates, and a greater entry (15--48%; 10 exps) when the perfusate contained bovine serum albumin (38 mg/ml). When the perfusate contained ovine or rat testicular fluid, or rat epididymal fluid at protein concentrations of 3 mg/ml or less, the entry of radioactivity into the epididymis was greater than when the perfusate contained 3 mg BSA/ml. The addition of ovine rete testis fluid protein (3 mg/ml to BSA (38 mg/ml) in the perfusate increased the uptake of radioactivity (58--106%; 6 exps). Radioactivity in blood was principally associated with testosterone (90, 95% total blood activity, 2 rats), whereas both [3H]testosterone (37, 41% total perfusate activity) and [3H]dihydrotestosterone (42, 63% total perfusate activity) was present in BSA-containing perfusates. The proportion of dihydrotestosterone appeared to increase when the perfusate contained protein of testicular origin."}
{"id": "PMID:469839", "title": "Steroidogenesis and prostaglandin synthesis by cultured bovine blastocysts.", "content": "Bovine blastocysts were collected at Days 13, 15 and 16 and placed in TCM-199 supplemented with 5% fetal calf serum; some blastocysts were immediately frozen while the others were cultured for 48 h and then frozen. Samples (tissue + medium, 5--12/group) were thawed, homogenized and analysed by radioimmunoassays. Measurable amounts of progesterone were found in all blastocysts but values were higher (P less than 0.01) after culture. Testosterone was not found in the cultured or uncultured blastocysts at Day 13, but was detectable on Days 15 and 16 and in greater amounts (P less than 0.05) in the cultured blastocysts. PGF and PGE-2 were increased (P less than 0.05) in the cultured blastocysts in all 3 days. Oestradiol was measurable in some but not all blastocysts. It is suggested that PG synthetase and enzymes capable of synthesizing progesterone, testosterone and, possibly, oestradiol are present in these early bovine blastocysts.", "contents": "Steroidogenesis and prostaglandin synthesis by cultured bovine blastocysts. Bovine blastocysts were collected at Days 13, 15 and 16 and placed in TCM-199 supplemented with 5% fetal calf serum; some blastocysts were immediately frozen while the others were cultured for 48 h and then frozen. Samples (tissue + medium, 5--12/group) were thawed, homogenized and analysed by radioimmunoassays. Measurable amounts of progesterone were found in all blastocysts but values were higher (P less than 0.01) after culture. Testosterone was not found in the cultured or uncultured blastocysts at Day 13, but was detectable on Days 15 and 16 and in greater amounts (P less than 0.05) in the cultured blastocysts. PGF and PGE-2 were increased (P less than 0.05) in the cultured blastocysts in all 3 days. Oestradiol was measurable in some but not all blastocysts. It is suggested that PG synthetase and enzymes capable of synthesizing progesterone, testosterone and, possibly, oestradiol are present in these early bovine blastocysts."}
{"id": "PMID:469840", "title": "Effects of cycloheximide on the uterine refractory state induced by 'nidatory' oestrogen in rats.", "content": "After priming with oestradiol, ovariectomized rats were given 6 days of progesterone treatment in which two doses of 50 ng oestradiol were given on Days 3 and 6. This basic treatment allows the oestradiol-induced (1st injection) disappearance of uterine sensitivity to decidual stimuli to occur. Cycloheximide could not mimic oestrogen action in the production of the uterine refractory state. However, a high dose (500 micrograms per animal) of this inhibitor given with the first injection of oestradiol allowed the uterus to remain in a neutral state and to respond to decidual induction after the second dose of oestradiol. By delaying the injection of cycloheximide after the first oestrogen treatment, protein synthesis requisite to the occurrence of uterine refractoriness would not take place within 12 h after the 'nidatory' oestrogen injection.", "contents": "Effects of cycloheximide on the uterine refractory state induced by 'nidatory' oestrogen in rats. After priming with oestradiol, ovariectomized rats were given 6 days of progesterone treatment in which two doses of 50 ng oestradiol were given on Days 3 and 6. This basic treatment allows the oestradiol-induced (1st injection) disappearance of uterine sensitivity to decidual stimuli to occur. Cycloheximide could not mimic oestrogen action in the production of the uterine refractory state. However, a high dose (500 micrograms per animal) of this inhibitor given with the first injection of oestradiol allowed the uterus to remain in a neutral state and to respond to decidual induction after the second dose of oestradiol. By delaying the injection of cycloheximide after the first oestrogen treatment, protein synthesis requisite to the occurrence of uterine refractoriness would not take place within 12 h after the 'nidatory' oestrogen injection."}
{"id": "PMID:469841", "title": "The lack of effect of ejaculate sequence on sex ratio in rabbits.", "content": "The influence of the order of the ejaculate on the sex ratio was determined by ejaculating 1 of 5 experimental males 20 times over a period of 2 to 8 h. The sex ratio was noted for the offspring of ejaculates 1, 5, 10, 15 and 20 in a total of 32 litters. The sex ratio of these offspring did not deviate signigicantly from 50:50 (P greater than 0.1) for any ejaculate in the sequence.", "contents": "The lack of effect of ejaculate sequence on sex ratio in rabbits. The influence of the order of the ejaculate on the sex ratio was determined by ejaculating 1 of 5 experimental males 20 times over a period of 2 to 8 h. The sex ratio was noted for the offspring of ejaculates 1, 5, 10, 15 and 20 in a total of 32 litters. The sex ratio of these offspring did not deviate signigicantly from 50:50 (P greater than 0.1) for any ejaculate in the sequence."}
{"id": "PMID:469842", "title": "Reduced prostaglandin levels in the semen of men with very high sperm concentrations.", "content": "The prostaglandin levels have been measured in a group of men with sperm concentrations greater than 300 X 10(6)/ml and compared with the levels in men with sperm concentrations of 50 to 150 X 10(6)/ml. The distribution of the PG levels in all groups was highly skewed but the data could be transformed to a normal distribution by taking logarithms. Comparison of the PG levels showed a highly significant lowering of the PG levels in the polyzoospermic group when compared wieth either of the groups with normal sperm concentrations.", "contents": "Reduced prostaglandin levels in the semen of men with very high sperm concentrations. The prostaglandin levels have been measured in a group of men with sperm concentrations greater than 300 X 10(6)/ml and compared with the levels in men with sperm concentrations of 50 to 150 X 10(6)/ml. The distribution of the PG levels in all groups was highly skewed but the data could be transformed to a normal distribution by taking logarithms. Comparison of the PG levels showed a highly significant lowering of the PG levels in the polyzoospermic group when compared wieth either of the groups with normal sperm concentrations."}
{"id": "PMID:469843", "title": "Effects of fluoroacetate on the testis of the rat.", "content": "Rats recieving 20, 6.6 or 2.2 p.p.m. sodium fluoroacetate in the drinking water were killed daily during the 7 days of treatment and at more widely spaced intervals in the succeeding 21 days. Testicular weight and ATP concentrations decreased in rats receiving 20 or 6 p.p.m. fluoroacetate, while citrate concentrations were elevated and morphological damage was seen in the testes of all the treated rats. Initial cellular changes common to the three treatment groups included altered appearance and decreased numbers of spermatids, and formation of spermatid and spermatocyte giant cells. At the two higher concentrations damage progressed to marked seminiferous tubule atrophy. Regeneration of the seminiferous tubules was complete by 7 days after treatment, in the rats given 2 p.p.m. but regeneration was not complete by Day 21 after treatment in those receiving the higher doses. Spermatogenesis was abnormal in some instances during the regneration period in these groups. The findings are consistent with impaired energy production via blockage of the Krebs cycle, and subsequent impairment of carbohydrate metabolism through the Embden-Meyerhof pathway.", "contents": "Effects of fluoroacetate on the testis of the rat. Rats recieving 20, 6.6 or 2.2 p.p.m. sodium fluoroacetate in the drinking water were killed daily during the 7 days of treatment and at more widely spaced intervals in the succeeding 21 days. Testicular weight and ATP concentrations decreased in rats receiving 20 or 6 p.p.m. fluoroacetate, while citrate concentrations were elevated and morphological damage was seen in the testes of all the treated rats. Initial cellular changes common to the three treatment groups included altered appearance and decreased numbers of spermatids, and formation of spermatid and spermatocyte giant cells. At the two higher concentrations damage progressed to marked seminiferous tubule atrophy. Regeneration of the seminiferous tubules was complete by 7 days after treatment, in the rats given 2 p.p.m. but regeneration was not complete by Day 21 after treatment in those receiving the higher doses. Spermatogenesis was abnormal in some instances during the regneration period in these groups. The findings are consistent with impaired energy production via blockage of the Krebs cycle, and subsequent impairment of carbohydrate metabolism through the Embden-Meyerhof pathway."}
{"id": "PMID:469844", "title": "A cytogenetic survey of men being investigated for subfertility.", "content": "A study of 336 men attending a subfertility clinic but otherwise unselected, and a further 12 men studied as a part of the investigation of the female partner, revealed 10 with major chromosome anomaly and 3 with unambiguous chromosomal variants. In addition to those with sex chromosome aneuploidy, an extra, small marker chromosome and D/D Robertsonian translocations, anomalies which have been reported in other studies, there were 7 men with rearrangements, including a paracentric inversion of chromosome 7 and an X/21 reciprocal translocation. These would have been difficult or impossible to identify without good banded preparations, suggesting that such rearrangement may be more frequent in association with subfertility than was appreciated.", "contents": "A cytogenetic survey of men being investigated for subfertility. A study of 336 men attending a subfertility clinic but otherwise unselected, and a further 12 men studied as a part of the investigation of the female partner, revealed 10 with major chromosome anomaly and 3 with unambiguous chromosomal variants. In addition to those with sex chromosome aneuploidy, an extra, small marker chromosome and D/D Robertsonian translocations, anomalies which have been reported in other studies, there were 7 men with rearrangements, including a paracentric inversion of chromosome 7 and an X/21 reciprocal translocation. These would have been difficult or impossible to identify without good banded preparations, suggesting that such rearrangement may be more frequent in association with subfertility than was appreciated."}
{"id": "PMID:469845", "title": "Androgen receptors in the rat epididymis and their hormonal control.", "content": "The concentrations of cytoplasmic receptor sites for androgens in the caput, corpus and cauda epididymidis, and the effect of ligation of the efferent ducts and testosterone treatment after bilateral castration on the concentration of receptors in the caput have been measured. Androgen receptors in the ventral prostate have been measured in the same animals for comparison. The caput has the highest concentration of receptor sites, the corpus the lowest. The ligation of the efferent ducts has no effect on this concentration which is dependent on testicular androgens. The present data do not yet allow explanation of the differential response of the different regions of the epididymis and of the other accessory glands to the administration of androgens.", "contents": "Androgen receptors in the rat epididymis and their hormonal control. The concentrations of cytoplasmic receptor sites for androgens in the caput, corpus and cauda epididymidis, and the effect of ligation of the efferent ducts and testosterone treatment after bilateral castration on the concentration of receptors in the caput have been measured. Androgen receptors in the ventral prostate have been measured in the same animals for comparison. The caput has the highest concentration of receptor sites, the corpus the lowest. The ligation of the efferent ducts has no effect on this concentration which is dependent on testicular androgens. The present data do not yet allow explanation of the differential response of the different regions of the epididymis and of the other accessory glands to the administration of androgens."}
{"id": "PMID:469846", "title": "Amino acid transport in the unfertilized and fertilized mouse egg.", "content": "The transport of methionine into unfertilized and fertilized mouse eggs appears to involve active transport mechanisms with similar Vmax, Km, substrate specificity and independence from Na+. An exchange diffusion system with a similar amino acid specificity to the uptake system has also been found in both types of egg. An estimate of 6.5 fmol has been made for the size of the total internal pool of exchangeable amino acids.", "contents": "Amino acid transport in the unfertilized and fertilized mouse egg. The transport of methionine into unfertilized and fertilized mouse eggs appears to involve active transport mechanisms with similar Vmax, Km, substrate specificity and independence from Na+. An exchange diffusion system with a similar amino acid specificity to the uptake system has also been found in both types of egg. An estimate of 6.5 fmol has been made for the size of the total internal pool of exchangeable amino acids."}
{"id": "PMID:469847", "title": "The effects of cooling to 5 degrees C and freezing and thawing on the ultrastructure of bull spermatozoa.", "content": "Semen from 6 bulls was examined under the transmission electron microscope immediately after collection, after dilution and cooling to 5 degrees C and after freezing and thawing. Conception rates were determined following artificial insemination of the frozen and thawed semen. Dilution and cooling to 5 degrees C caused acrosomal swelling in about 50% of the spermatozoa. Subsequent freezing and thawing caused considerable ultrastructural changes to the acrosomes (disruption of the plasma and outer acrosomal membranes and dispersion of the acrosomal contents) and middle pieces (breakage of the plasma membrane and a reduction in the electron density of the mitochondrial matrix) of a high proportion of spermatozoa. The average non-return rate following insemination of semen from 5 of the bulls was 61.6% and higher (P greater than 0.001) than for the sixth bull (15%). Although this difference in semen viability was also demonstrated in the structural studies (acrosome, P greater than 0.05: middle piece, P greater than 0.001), more work is required to assess the relationship between structure and function of spermatozoa.", "contents": "The effects of cooling to 5 degrees C and freezing and thawing on the ultrastructure of bull spermatozoa. Semen from 6 bulls was examined under the transmission electron microscope immediately after collection, after dilution and cooling to 5 degrees C and after freezing and thawing. Conception rates were determined following artificial insemination of the frozen and thawed semen. Dilution and cooling to 5 degrees C caused acrosomal swelling in about 50% of the spermatozoa. Subsequent freezing and thawing caused considerable ultrastructural changes to the acrosomes (disruption of the plasma and outer acrosomal membranes and dispersion of the acrosomal contents) and middle pieces (breakage of the plasma membrane and a reduction in the electron density of the mitochondrial matrix) of a high proportion of spermatozoa. The average non-return rate following insemination of semen from 5 of the bulls was 61.6% and higher (P greater than 0.001) than for the sixth bull (15%). Although this difference in semen viability was also demonstrated in the structural studies (acrosome, P greater than 0.05: middle piece, P greater than 0.001), more work is required to assess the relationship between structure and function of spermatozoa."}
{"id": "PMID:469848", "title": "Two intersex dogs with mosaicism.", "content": "Two intersex dogs with various degrees of mosaicism in the somatic tissues are described. Normal female cells as well as Y-bearing cells with aneuploidy and other abnormalities were involved. In the Red setter an ovarian cortex had developed in mosaic gonads with 8 and 10% of Y-bearing cells. Slight masculinization of the medullae corresponded with some external virilization. These observations support the existence of a threshold proportion of Y-bearing cells for testicular differentiation. Fragments of chromatin in 36% or fewer of the cells in the Cocker spaniel may have been translocated Y-chromosome material.", "contents": "Two intersex dogs with mosaicism. Two intersex dogs with various degrees of mosaicism in the somatic tissues are described. Normal female cells as well as Y-bearing cells with aneuploidy and other abnormalities were involved. In the Red setter an ovarian cortex had developed in mosaic gonads with 8 and 10% of Y-bearing cells. Slight masculinization of the medullae corresponded with some external virilization. These observations support the existence of a threshold proportion of Y-bearing cells for testicular differentiation. Fragments of chromatin in 36% or fewer of the cells in the Cocker spaniel may have been translocated Y-chromosome material."}
{"id": "PMID:469849", "title": "The effects of corpus luteum removal during gestation on parturition in the tammar wallaby (Macorpus eugenii).", "content": "Removal of the corpus luteum of pregnancy of the tammar wallaby interfered with successful parturition if carried out before Day 17 of the 27-day pregnancy. After removal at Days 17 and 21, 40% of animals gave birth but pouch young died with 24 h; if performed at Days 23 or 25, pouch young survived. However, surgery also affected sham-operated controls if performed between Days 15 and 21. In control animals, sodium pentobarbitone followed by halothane anaesthesia was the least disruptive anaesthetic procedure. This study shows that the corpus luteum has an essential role in parturition and subsequent survival of the neonate in the pouch.", "contents": "The effects of corpus luteum removal during gestation on parturition in the tammar wallaby (Macorpus eugenii). Removal of the corpus luteum of pregnancy of the tammar wallaby interfered with successful parturition if carried out before Day 17 of the 27-day pregnancy. After removal at Days 17 and 21, 40% of animals gave birth but pouch young died with 24 h; if performed at Days 23 or 25, pouch young survived. However, surgery also affected sham-operated controls if performed between Days 15 and 21. In control animals, sodium pentobarbitone followed by halothane anaesthesia was the least disruptive anaesthetic procedure. This study shows that the corpus luteum has an essential role in parturition and subsequent survival of the neonate in the pouch."}
{"id": "PMID:469850", "title": "Synthesis of 'decidualization-associated protein' in tissues of the rat uterus and placenta during pregnancy.", "content": "The synthesis of a soluble protein (referred to as 'decidualization-associated protein', DAP), has been examined in uterine and placental tissues of rats during pregnancy by means of polyacrylamide gel electrophoretic analysis of [3H]leucine-labelled soluble proteins. No synthesis of the protein was detected in non-implantation regions of the uterus. In implantation site tissue, no synthesis was detected on Days 6 or 7 of pregnancy. Only slight synthesis was present in the endometrium on Day 8, but synthesis rose rapidly from Days 9 to 12 in both the endometrium and myometrium although differences in the rates of increase were observed. Synthesis fell from Day 12 to 14 in both tissues. Synthesis by the myometrium was entirely localized in the mesometrial region, which contains the metrial gland. After Day 12, when the endometrium is represented by the chorioallantoic placenta, synthesis was examined in the labyrinthine and the decidua basalis/basal zone placenta tissues. No synthesis of 'DAP' was detected in the labyrinthine placenta from Day 16 of pregnancy. Synthesis observed in the decidua basalis/basal zone placenta fell dramatically from Day 14 to 20. The pattern of synthesis of 'DAP' during pregnancy suggests a role in the establishment of the chorioallantoic placenta and metrial gland in the rat.", "contents": "Synthesis of 'decidualization-associated protein' in tissues of the rat uterus and placenta during pregnancy. The synthesis of a soluble protein (referred to as 'decidualization-associated protein', DAP), has been examined in uterine and placental tissues of rats during pregnancy by means of polyacrylamide gel electrophoretic analysis of [3H]leucine-labelled soluble proteins. No synthesis of the protein was detected in non-implantation regions of the uterus. In implantation site tissue, no synthesis was detected on Days 6 or 7 of pregnancy. Only slight synthesis was present in the endometrium on Day 8, but synthesis rose rapidly from Days 9 to 12 in both the endometrium and myometrium although differences in the rates of increase were observed. Synthesis fell from Day 12 to 14 in both tissues. Synthesis by the myometrium was entirely localized in the mesometrial region, which contains the metrial gland. After Day 12, when the endometrium is represented by the chorioallantoic placenta, synthesis was examined in the labyrinthine and the decidua basalis/basal zone placenta tissues. No synthesis of 'DAP' was detected in the labyrinthine placenta from Day 16 of pregnancy. Synthesis observed in the decidua basalis/basal zone placenta fell dramatically from Day 14 to 20. The pattern of synthesis of 'DAP' during pregnancy suggests a role in the establishment of the chorioallantoic placenta and metrial gland in the rat."}
{"id": "PMID:469851", "title": "Comparison of the embryo survival rate in heifers following artificial insemination, non-surgical blastocyst transfer or both.", "content": "In Exp. 1, embryo survival rates of 45 and 47% were recorded after artificial insemination and ipsilateral transfer respectively. In Exp. 2, pregnancy rates of 62 and 60% were recorded after artificial insemination and contralateral transfer to inseminated recipients respectively. In this experiment the contralateral transferred embryo survival rate was 44%. Transferred embryo survival was lower overall when donors and recipients were out of phase by 1 day than when exactly synchronous.", "contents": "Comparison of the embryo survival rate in heifers following artificial insemination, non-surgical blastocyst transfer or both. In Exp. 1, embryo survival rates of 45 and 47% were recorded after artificial insemination and ipsilateral transfer respectively. In Exp. 2, pregnancy rates of 62 and 60% were recorded after artificial insemination and contralateral transfer to inseminated recipients respectively. In this experiment the contralateral transferred embryo survival rate was 44%. Transferred embryo survival was lower overall when donors and recipients were out of phase by 1 day than when exactly synchronous."}
{"id": "PMID:469852", "title": "Changes in uterine phosphatase levels in mice deprived of LH during early pregnancy.", "content": "Mice injected with normal rabbit serum on Day 4 of gestation showed a progressive increase in specific alkaline and acid phosphatase activities on Days 6 and 7. Mice injected with antiserum to LH on Day 4 showed a significant decrease in specific alkaline phosphatase activity estimated biochemically on Days 6, 7 and 8, but this decrease was histochemically evident only on Day 8. Similarly, acid phosphatase activity in antiserum-treated mice was significantly increased on Days 6, 7 and 8 when estimated biochemically, but only on Day 8 when studied histochemically.", "contents": "Changes in uterine phosphatase levels in mice deprived of LH during early pregnancy. Mice injected with normal rabbit serum on Day 4 of gestation showed a progressive increase in specific alkaline and acid phosphatase activities on Days 6 and 7. Mice injected with antiserum to LH on Day 4 showed a significant decrease in specific alkaline phosphatase activity estimated biochemically on Days 6, 7 and 8, but this decrease was histochemically evident only on Day 8. Similarly, acid phosphatase activity in antiserum-treated mice was significantly increased on Days 6, 7 and 8 when estimated biochemically, but only on Day 8 when studied histochemically."}
{"id": "PMID:469853", "title": "Early pregnancy diagnosis in the ewe, based on milk progesterone levels.", "content": "Plasma and milk progesterone concentrations in pregnant sheep (18--22 days after mating) were similar, about 3.7 ng/ml whereas values in non-pregnant sheep were less than 1 ng/ml. Lambing results indicated identical accuracy for both methods (82 and 84% in 2 flocks). The accuracy was 92--100% for ewes diagnosed non-pregnant in the breeding season, but for ewes tested in the non-breeding season the diagnosis of non-pregnancy according to milk progesterone levels was only 50% accurate.", "contents": "Early pregnancy diagnosis in the ewe, based on milk progesterone levels. Plasma and milk progesterone concentrations in pregnant sheep (18--22 days after mating) were similar, about 3.7 ng/ml whereas values in non-pregnant sheep were less than 1 ng/ml. Lambing results indicated identical accuracy for both methods (82 and 84% in 2 flocks). The accuracy was 92--100% for ewes diagnosed non-pregnant in the breeding season, but for ewes tested in the non-breeding season the diagnosis of non-pregnancy according to milk progesterone levels was only 50% accurate."}
{"id": "PMID:469854", "title": "Presence of placental lactogen in bovine conceptuses before attachment.", "content": "Placental lactogen has been detected by radioreceptor assay in bovine conceptuses colletected between 17 and 25 days post coitum, at or shortly after the time of appearance of binucleate cells in the bovine trophectoderm, but before attachment or implantation.", "contents": "Presence of placental lactogen in bovine conceptuses before attachment. Placental lactogen has been detected by radioreceptor assay in bovine conceptuses colletected between 17 and 25 days post coitum, at or shortly after the time of appearance of binucleate cells in the bovine trophectoderm, but before attachment or implantation."}
{"id": "PMID:469855", "title": "Preliminary observations on the effects of 19-hydroxy prostaglandin E-1 on the activity of the human myometrium in vitro.", "content": "The principal prostaglandin of human semen, 19 hydroxy PGE-1, inhibited spontaneous contractile activity of myometrium from non-pregnant women in vitro. This inhibition was similar to that caused by PGE-1. The inhibitory effects of natural 19 hydroxy PGE-1, prepared from semen of Macaca arctoides, were similar to those of synthetic 19 hydroxy PGE-1.", "contents": "Preliminary observations on the effects of 19-hydroxy prostaglandin E-1 on the activity of the human myometrium in vitro. The principal prostaglandin of human semen, 19 hydroxy PGE-1, inhibited spontaneous contractile activity of myometrium from non-pregnant women in vitro. This inhibition was similar to that caused by PGE-1. The inhibitory effects of natural 19 hydroxy PGE-1, prepared from semen of Macaca arctoides, were similar to those of synthetic 19 hydroxy PGE-1."}
{"id": "PMID:469857", "title": "Steroid receptors in the canine epididymis.", "content": "High-affinity and low-capacity molecules that bind 5 alpha-dihydrotestosterone and oestradiol-17 beta have been demonstrated in the canine epididymis. The molecules were shown to be proteinaceous in nature, thermolabile and sedimented in a sucrose density gradient with a coefficient of 8S with respect to bovine serum albumin. Only minor differences were found for dissociation constants for oestradio-17 beta (3.5 +/- 1.74 S 10(-10) M) and 5 alpha-dihydrotestosterone (4.2 +/- 10.75 X 10(-10) M) (means +/- s.d.) in the three anatomically distinct regions of the epididymis.", "contents": "Steroid receptors in the canine epididymis. High-affinity and low-capacity molecules that bind 5 alpha-dihydrotestosterone and oestradiol-17 beta have been demonstrated in the canine epididymis. The molecules were shown to be proteinaceous in nature, thermolabile and sedimented in a sucrose density gradient with a coefficient of 8S with respect to bovine serum albumin. Only minor differences were found for dissociation constants for oestradio-17 beta (3.5 +/- 1.74 S 10(-10) M) and 5 alpha-dihydrotestosterone (4.2 +/- 10.75 X 10(-10) M) (means +/- s.d.) in the three anatomically distinct regions of the epididymis."}
{"id": "PMID:469858", "title": "Uterine blood flow of cows during the oestrous cycle and early pregnancy: effect of the conceptus on the uterine blood supply.", "content": "Blood flow to each uterine horn of cows during the oestrous cycle and early pregnancy was determined daily by use of electromagnetic blood flow probes placed around both middle uterine arteries. The pattern of blood flow to uteri of pregnant and non-pregnant cows was similar until Day 14 after mating or oestrus. Between Days 14 and 18 of pregnancy blood flow to the uterine horn containing the conceptus increased (P less than 0.01) 2- to 3-fold, whereas blood flow to the non-gravid uterine horn in these cows remained constant. No corresponding increase in blood flow to the uterine horn ipsilateral to the ovary bearing the CL was observed in non-pregnant cows during this 4-day period. By Day 19 of pregnancy, blood flow to the gravid uterine horn had returned to a level similar to that observed on Day 13. Blood flow to both uterine horns of pregnant cows remained constant from Days 19 to 25 and then increased to the gravid horn (P less than 0.01) markedly until Day 30 whereas blood flow to the non-gravid horn remained low. Uterine blood flow during the oestrous cycle of non-pregnant cows was positively correlated (P less than 0.01) with systemic concentrations of oestradiol and the ratio of oestradiol (pg/ml) to progesterone (ng/ml). There was no association between oestradiol concentrations and blood flow to the gravid uterine horn. These data indicate local control of uterine blood flow by the bovine conceptus which may function to create optimal conditions for the continuation of pregnancy.", "contents": "Uterine blood flow of cows during the oestrous cycle and early pregnancy: effect of the conceptus on the uterine blood supply. Blood flow to each uterine horn of cows during the oestrous cycle and early pregnancy was determined daily by use of electromagnetic blood flow probes placed around both middle uterine arteries. The pattern of blood flow to uteri of pregnant and non-pregnant cows was similar until Day 14 after mating or oestrus. Between Days 14 and 18 of pregnancy blood flow to the uterine horn containing the conceptus increased (P less than 0.01) 2- to 3-fold, whereas blood flow to the non-gravid uterine horn in these cows remained constant. No corresponding increase in blood flow to the uterine horn ipsilateral to the ovary bearing the CL was observed in non-pregnant cows during this 4-day period. By Day 19 of pregnancy, blood flow to the gravid uterine horn had returned to a level similar to that observed on Day 13. Blood flow to both uterine horns of pregnant cows remained constant from Days 19 to 25 and then increased to the gravid horn (P less than 0.01) markedly until Day 30 whereas blood flow to the non-gravid horn remained low. Uterine blood flow during the oestrous cycle of non-pregnant cows was positively correlated (P less than 0.01) with systemic concentrations of oestradiol and the ratio of oestradiol (pg/ml) to progesterone (ng/ml). There was no association between oestradiol concentrations and blood flow to the gravid uterine horn. These data indicate local control of uterine blood flow by the bovine conceptus which may function to create optimal conditions for the continuation of pregnancy."}
{"id": "PMID:469860", "title": "Peripheral plasma androstenedione and testosterone concentrations in bulls before and during puberty.", "content": "Peripheral plasma samples were collected from 37 young bulls every other month from 2 to 12 months of age. Androstenedione and testosterone were measured by radioimmunoassays. Androstenedione values increased from 2 to 4 months of age (525 +/- 296 (s.d.) pg/ml) and then decreased. Testosterone concentrations increased regularly from 2 months (0.17 +/- 0.14 ng/ml) to 6 months (2.79 +/- 1.29 ng/ml) and then remained at levels around 3 ng/ml. Coefficients of correlation between the hormone concentrations were significant only at 6 months and thereafter.", "contents": "Peripheral plasma androstenedione and testosterone concentrations in bulls before and during puberty. Peripheral plasma samples were collected from 37 young bulls every other month from 2 to 12 months of age. Androstenedione and testosterone were measured by radioimmunoassays. Androstenedione values increased from 2 to 4 months of age (525 +/- 296 (s.d.) pg/ml) and then decreased. Testosterone concentrations increased regularly from 2 months (0.17 +/- 0.14 ng/ml) to 6 months (2.79 +/- 1.29 ng/ml) and then remained at levels around 3 ng/ml. Coefficients of correlation between the hormone concentrations were significant only at 6 months and thereafter."}
{"id": "PMID:469859", "title": "Trophoblastin, an antiluteolytic protein present in early pregnancy in sheep.", "content": "Trophoblastin, an antiluteolytic component from the embryo, was identified in the ewe by the means of intrauterine injections of homogenates from trophoblasts at 14--16 days pregnancy. Homogenates from embryos and their membranes at 21--23 days pregnancy did not extend the life of the corpus luteum, suggesting that trophoblastin synthesis occurs for only a short period. The trophoblastin was thermolabile (80 degrees C for 30 min) and inactivated by pronase. Treatment of ewes with oCS, hCG, and extracts of 120-day placentae did not affect the time of luteolysis. The protein appears to be insoluble at pH 7 or 8, but to dissolve readily at pH 9.6. After injection of homogenates or extracts from 15--16-day-old trophoblasts, the initial CL were maintained for more than 1 month in most cyclic recipient ewes. Surgical removal of embryos at 21--23 days resulted in luteal maintenace for more than 1 month in over 50% of the operated animals. All the maintained CL were secretory although their average weight was about one-half of that CL of normal pregnancy, suggesting the existence of complementary luteotrophic placental factors. The uteri of most of these pseudopregnant ewes were distended with a clear, sterile fluid.", "contents": "Trophoblastin, an antiluteolytic protein present in early pregnancy in sheep. Trophoblastin, an antiluteolytic component from the embryo, was identified in the ewe by the means of intrauterine injections of homogenates from trophoblasts at 14--16 days pregnancy. Homogenates from embryos and their membranes at 21--23 days pregnancy did not extend the life of the corpus luteum, suggesting that trophoblastin synthesis occurs for only a short period. The trophoblastin was thermolabile (80 degrees C for 30 min) and inactivated by pronase. Treatment of ewes with oCS, hCG, and extracts of 120-day placentae did not affect the time of luteolysis. The protein appears to be insoluble at pH 7 or 8, but to dissolve readily at pH 9.6. After injection of homogenates or extracts from 15--16-day-old trophoblasts, the initial CL were maintained for more than 1 month in most cyclic recipient ewes. Surgical removal of embryos at 21--23 days resulted in luteal maintenace for more than 1 month in over 50% of the operated animals. All the maintained CL were secretory although their average weight was about one-half of that CL of normal pregnancy, suggesting the existence of complementary luteotrophic placental factors. The uteri of most of these pseudopregnant ewes were distended with a clear, sterile fluid."}
{"id": "PMID:469861", "title": "Detection of early pregnancy in sheep by the rosette inhibition test.", "content": "The rossette inhibition test, an established test for determining the immunosuppressive potential of antilymphocyte serum, has been applied to the serum of sheep after mating. The rosette inhibition titre was much higher (12--26) in 7 sheep which were fertilized and remained pregnant for up to 21 days than in 5 sterile ewes mated with intact rams (8--10). The difference was apparent by 24 h after mating. One ewe had high titres for 6 days after mating but these then dropped and she returned to oestrus; early embryonic loss was suspected. Another ewe which returned to oestrus had consistently low titres. The results indicate that the rosette inhibition test can be used to detect fertilization, early embryonic death and continued pregnancy in sheep.", "contents": "Detection of early pregnancy in sheep by the rosette inhibition test. The rossette inhibition test, an established test for determining the immunosuppressive potential of antilymphocyte serum, has been applied to the serum of sheep after mating. The rosette inhibition titre was much higher (12--26) in 7 sheep which were fertilized and remained pregnant for up to 21 days than in 5 sterile ewes mated with intact rams (8--10). The difference was apparent by 24 h after mating. One ewe had high titres for 6 days after mating but these then dropped and she returned to oestrus; early embryonic loss was suspected. Another ewe which returned to oestrus had consistently low titres. The results indicate that the rosette inhibition test can be used to detect fertilization, early embryonic death and continued pregnancy in sheep."}
{"id": "PMID:469862", "title": "Effect of prostaglandin F-2 alpha on plasma levels of progesterone and pregnenolone in the hysterectomized guinea-pig.", "content": "Repeated injections of PGF-2 alpha were given to hysterectomized guinea-pigs. Within 12 h after the first injection, plasma progesterone levels were significantly reduced (P less than 0.001) and declined to less than 1 ng/ml by the 4th day after the end of PGF-2 alpha treatment. A decline in plasma levels of pregnenolone paralleled that of progesterone. PGF-2 alpha treatment did not affect the metabolic clearance rate of [3H]pregnenolone, but the converions of [3H]pregnenolone to [3H]progesterone was reduced by about 50%.", "contents": "Effect of prostaglandin F-2 alpha on plasma levels of progesterone and pregnenolone in the hysterectomized guinea-pig. Repeated injections of PGF-2 alpha were given to hysterectomized guinea-pigs. Within 12 h after the first injection, plasma progesterone levels were significantly reduced (P less than 0.001) and declined to less than 1 ng/ml by the 4th day after the end of PGF-2 alpha treatment. A decline in plasma levels of pregnenolone paralleled that of progesterone. PGF-2 alpha treatment did not affect the metabolic clearance rate of [3H]pregnenolone, but the converions of [3H]pregnenolone to [3H]progesterone was reduced by about 50%."}
{"id": "PMID:469863", "title": "Effect of prostaglandin F-2 alpha on ovarian enzyme activity in the hysterectomized guinea-pig.", "content": "The enzymes studied were cholesterol esterase, cholesterol ester synthetase 3 beta-hydroxysteroid dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. PGF-2 alpha reduced the activities of 3 beta-hydroxysteroid dehydrogenase and cholesterol esterase but did not affect those of cholesterol ester synthetase of 20 alpha-hydroxysteroid dehydrogenase.", "contents": "Effect of prostaglandin F-2 alpha on ovarian enzyme activity in the hysterectomized guinea-pig. The enzymes studied were cholesterol esterase, cholesterol ester synthetase 3 beta-hydroxysteroid dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. PGF-2 alpha reduced the activities of 3 beta-hydroxysteroid dehydrogenase and cholesterol esterase but did not affect those of cholesterol ester synthetase of 20 alpha-hydroxysteroid dehydrogenase."}
{"id": "PMID:469864", "title": "Luteinization of bovine granulosa cells and corpus luteum formation associated with loss of androgen-aromatizing ability.", "content": "The relative aromatizing ability of bovine luteinizing granulosa cells and dispersed luteal cells in tissue culture was studied. Luteinization of granulosa cells, as indicated by steadily increasing progesterone production (from 50 to 300 ng/10(5) cells/day over 4--5 days), was accompanied by a dramatic reduction in their capacity to aromatize exogenous androgen; oestradiol-17 beta production falling from 200 to less than 10 ng/10(5) cells/day over 4--5 days. Luteal cells also had only a very limited capacity to aromatize exogenous androgen, maximum oestradiol-17 beta production being less than 600 pg/10(5) cells/day. The loss in aromatizing capacity of granulosa cells during luteinization was also reflected in the relative endogenous steroid content of non-luteinized granulosa cells and luteal tissue, the former containing high levels of oestradiol-17 beta, less than or equal to 28 ng/mg protein, while the latter, although containing substantial amounts of testosterone, less than or equal to 5.7 ng/g tissue, contained very little oestradiol-17 beta, less than or equal to 0.35 ngG TISSUE. These findings suggest that luteinization of bovine granulosa cells and subsequent corpus luteum formation is associated with a loss in androgen aromatase activity.", "contents": "Luteinization of bovine granulosa cells and corpus luteum formation associated with loss of androgen-aromatizing ability. The relative aromatizing ability of bovine luteinizing granulosa cells and dispersed luteal cells in tissue culture was studied. Luteinization of granulosa cells, as indicated by steadily increasing progesterone production (from 50 to 300 ng/10(5) cells/day over 4--5 days), was accompanied by a dramatic reduction in their capacity to aromatize exogenous androgen; oestradiol-17 beta production falling from 200 to less than 10 ng/10(5) cells/day over 4--5 days. Luteal cells also had only a very limited capacity to aromatize exogenous androgen, maximum oestradiol-17 beta production being less than 600 pg/10(5) cells/day. The loss in aromatizing capacity of granulosa cells during luteinization was also reflected in the relative endogenous steroid content of non-luteinized granulosa cells and luteal tissue, the former containing high levels of oestradiol-17 beta, less than or equal to 28 ng/mg protein, while the latter, although containing substantial amounts of testosterone, less than or equal to 5.7 ng/g tissue, contained very little oestradiol-17 beta, less than or equal to 0.35 ngG TISSUE. These findings suggest that luteinization of bovine granulosa cells and subsequent corpus luteum formation is associated with a loss in androgen aromatase activity."}
{"id": "PMID:469865", "title": "Fibrocystic disease of the breast.", "content": "Fibrocystic disease is the most common type of lesion in the female breast. It is a common dumping ground for a variety of distinct clinical and histologic entities which require different modalities of therapy and which have different malignant transformation potentials. In general, fibrocystic disease is managed medically unless a dominant lump or other adverse symptom develops, in which case a biopsy is performed. The exception to this rule is a cyst which can be safely aspirated under controlled conditions. If, on biopsy, one of the histologic types of fibrocystic disease called \"precancerous mastopathy\" is found, which has a high potential for malignant transformation, then serious consideration must be given to definitive surgical procedures, especially if the patient has other risk factors that would increase her risk of developing breast cancer even further.", "contents": "Fibrocystic disease of the breast. Fibrocystic disease is the most common type of lesion in the female breast. It is a common dumping ground for a variety of distinct clinical and histologic entities which require different modalities of therapy and which have different malignant transformation potentials. In general, fibrocystic disease is managed medically unless a dominant lump or other adverse symptom develops, in which case a biopsy is performed. The exception to this rule is a cyst which can be safely aspirated under controlled conditions. If, on biopsy, one of the histologic types of fibrocystic disease called \"precancerous mastopathy\" is found, which has a high potential for malignant transformation, then serious consideration must be given to definitive surgical procedures, especially if the patient has other risk factors that would increase her risk of developing breast cancer even further."}
{"id": "PMID:469871", "title": "Law and medical ethics.", "content": "Summarising the interrelationship between law and medical ethics, I would say that in cases which do not touch the patient's body or integrity, such as professional secrecy, statutory law may take precedence over rules of medical ethics. But in cases where the human subject becomes a victim because of domestic statutory laws which are in contradiction with medical ethics, the medical practitioners should insist on adhering to their professional standards in such a way that the legislators will have to adapt their legislations to the laws of humanity and public conscience. Legislators, as well as medical practitioners, should not forget that the term 'being' is preceded and qualified by 'human'.", "contents": "Law and medical ethics. Summarising the interrelationship between law and medical ethics, I would say that in cases which do not touch the patient's body or integrity, such as professional secrecy, statutory law may take precedence over rules of medical ethics. But in cases where the human subject becomes a victim because of domestic statutory laws which are in contradiction with medical ethics, the medical practitioners should insist on adhering to their professional standards in such a way that the legislators will have to adapt their legislations to the laws of humanity and public conscience. Legislators, as well as medical practitioners, should not forget that the term 'being' is preceded and qualified by 'human'."}
{"id": "PMID:469872", "title": "The nature of confidentiality.", "content": "This paper examines confidentiality and its nature and analyses the guidelines laid down by the Hippocratic Oath as well as the British and World Medical Associations for maintaining such confidentiality between doctor and patient. There are exceptions to practically any code of rules and this is true also for confidentiality. Some of these exceptions make it appear that very little is confidential. The three values implicit in confidentiality would seem to be privacy, confidence and secrecy. Each of these values is discussed and developed in this paper. In conclusion, the question is suggested that maybe in the face of death, doctor and patient need to re-examine the pre-suppositions of privacy, confidence and secrecy on which the confidential relationship is based.", "contents": "The nature of confidentiality. This paper examines confidentiality and its nature and analyses the guidelines laid down by the Hippocratic Oath as well as the British and World Medical Associations for maintaining such confidentiality between doctor and patient. There are exceptions to practically any code of rules and this is true also for confidentiality. Some of these exceptions make it appear that very little is confidential. The three values implicit in confidentiality would seem to be privacy, confidence and secrecy. Each of these values is discussed and developed in this paper. In conclusion, the question is suggested that maybe in the face of death, doctor and patient need to re-examine the pre-suppositions of privacy, confidence and secrecy on which the confidential relationship is based."}
{"id": "PMID:469873", "title": "Deciding the care of severely malformed or dying infants.", "content": "Suffering patients (when able), grieving families and compassionate physicians have always sought the least detrimental alternative while deciding care in the face of tragedy. Modern medical technology has brought great benefits to patients but has blurred traditional concepts of life and death and created new dilemmas for practising doctors. While this technology has given doctors great control over living and dying, their dominance in critical decision making is being challenged. More and more their decisions are liable to public and legal scrutiny, intense publicity by the news media and exploitation by lobbyists with opposing aims. Increasing pressure of this kind may deflect the physician form his primary responsibility to patients and their families. For infants with gross malformations or a distressing terminal illness we believe that the parents and their doctors must be allowed primary decisional power even if the chosen course of action involves the death of the infant. Choices for death should be permitted but only after suitable family and professional consultation. Some general guidelines are suggested. As these situations are so varied and so complex, much latitude in decision-making should be expected and tolerated.", "contents": "Deciding the care of severely malformed or dying infants. Suffering patients (when able), grieving families and compassionate physicians have always sought the least detrimental alternative while deciding care in the face of tragedy. Modern medical technology has brought great benefits to patients but has blurred traditional concepts of life and death and created new dilemmas for practising doctors. While this technology has given doctors great control over living and dying, their dominance in critical decision making is being challenged. More and more their decisions are liable to public and legal scrutiny, intense publicity by the news media and exploitation by lobbyists with opposing aims. Increasing pressure of this kind may deflect the physician form his primary responsibility to patients and their families. For infants with gross malformations or a distressing terminal illness we believe that the parents and their doctors must be allowed primary decisional power even if the chosen course of action involves the death of the infant. Choices for death should be permitted but only after suitable family and professional consultation. Some general guidelines are suggested. As these situations are so varied and so complex, much latitude in decision-making should be expected and tolerated."}
{"id": "PMID:469874", "title": "Learning about death.", "content": "This paper outlines briefly some of the research which has been carried out on attitudes to terminal illness and its care. The writer feels that not enough effort is being put into the teaching of this subject in our medical schools and Universities, and that doctors themselves are the ones who often wish to 'duck' the issue of dealing with disability and the dying. However, with the increasing awareness, through both the research and the growing allied literature, the writer feels that there is no longer any excuse for omitting this subject from the curricula for doctors and nurses in training.", "contents": "Learning about death. This paper outlines briefly some of the research which has been carried out on attitudes to terminal illness and its care. The writer feels that not enough effort is being put into the teaching of this subject in our medical schools and Universities, and that doctors themselves are the ones who often wish to 'duck' the issue of dealing with disability and the dying. However, with the increasing awareness, through both the research and the growing allied literature, the writer feels that there is no longer any excuse for omitting this subject from the curricula for doctors and nurses in training."}
{"id": "PMID:469880", "title": "Familial caudal regression anomalad and maternal diabetes.", "content": "A family is reported which a diabetic woman gave birth to two children with the caudal regression anomalad (CRA). There were no obvious genetic factors. This is the first reported familial case of CRA with maternal diabetes.", "contents": "Familial caudal regression anomalad and maternal diabetes. A family is reported which a diabetic woman gave birth to two children with the caudal regression anomalad (CRA). There were no obvious genetic factors. This is the first reported familial case of CRA with maternal diabetes."}
{"id": "PMID:469881", "title": "Tissue typing amniotic fluid cells: potential use for detection of contaminating maternal cells.", "content": "The presence of contaminating maternal cells in amniotic fluid is an important, though infrequent, cause of error in karyotyping the fetus. A method of detecting contaminating maternal cells in amniocentesis specimens by determining the HLA phenotype of the cells of amniotic fluid and the mother is described. Tissue typing of 15 amniocentesis specimens was performed, and in 14 cases the fetal origin of the cells was established. In one case, the results of tissue typing suggested maternal cell contamination, though this had not been suspected from chromosome studies of the amniotic fluid cell cultures. Other possible uses for tissue typing of amniotic fluid specimens for prenatal diagnosis are also described.", "contents": "Tissue typing amniotic fluid cells: potential use for detection of contaminating maternal cells. The presence of contaminating maternal cells in amniotic fluid is an important, though infrequent, cause of error in karyotyping the fetus. A method of detecting contaminating maternal cells in amniocentesis specimens by determining the HLA phenotype of the cells of amniotic fluid and the mother is described. Tissue typing of 15 amniocentesis specimens was performed, and in 14 cases the fetal origin of the cells was established. In one case, the results of tissue typing suggested maternal cell contamination, though this had not been suspected from chromosome studies of the amniotic fluid cell cultures. Other possible uses for tissue typing of amniotic fluid specimens for prenatal diagnosis are also described."}
{"id": "PMID:469883", "title": "Tuberous sclerosis: possible modification of phenotypic expression by an unlinked dominant gene.", "content": "A unique pedigree is presented which shows tuberous sclerosis in three generations of a family, in which two heterozygotes for the mutant gene were found to be clinically asymptomatic. A genetic model is proposed to explain these findings based upon the segregation of a second unlinked autosomal dominant gene modifying the expression of the gene for tuberous sclerosis.", "contents": "Tuberous sclerosis: possible modification of phenotypic expression by an unlinked dominant gene. A unique pedigree is presented which shows tuberous sclerosis in three generations of a family, in which two heterozygotes for the mutant gene were found to be clinically asymptomatic. A genetic model is proposed to explain these findings based upon the segregation of a second unlinked autosomal dominant gene modifying the expression of the gene for tuberous sclerosis."}
{"id": "PMID:469886", "title": "Serum creatine kinase levels in normal females.", "content": "Serum creatine kinase levels were determined in 75 girls (age range, one month to 15 years) and 200 normal adult women (age range, 18 to 50 years). The values ranged from 12.5 to 80 IU/1 in girls and 19 to 155 IU/1 in adult females. The SCK level appeared to increase with age from 1 to 15 years, after which the level remained fairly constant. These data should be helpful in the detection of carriers of X-linked forms of muscular dystrophy.", "contents": "Serum creatine kinase levels in normal females. Serum creatine kinase levels were determined in 75 girls (age range, one month to 15 years) and 200 normal adult women (age range, 18 to 50 years). The values ranged from 12.5 to 80 IU/1 in girls and 19 to 155 IU/1 in adult females. The SCK level appeared to increase with age from 1 to 15 years, after which the level remained fairly constant. These data should be helpful in the detection of carriers of X-linked forms of muscular dystrophy."}
{"id": "PMID:469887", "title": "Beta-thalassaemia: increased chromosomal anomalies in lymphocyte cultures.", "content": "Lymphocyte cultures from homozygotes for the beta-thalassaemia gene were found to contain chromosomal gaps, breaks, and rearrangements more often than those from controls. Culture time seemed to have a determinant effect on the results. The possible influence of folic acid deficiency is discussed.", "contents": "Beta-thalassaemia: increased chromosomal anomalies in lymphocyte cultures. Lymphocyte cultures from homozygotes for the beta-thalassaemia gene were found to contain chromosomal gaps, breaks, and rearrangements more often than those from controls. Culture time seemed to have a determinant effect on the results. The possible influence of folic acid deficiency is discussed."}
{"id": "PMID:469888", "title": "Meiosis in two human reciprocal translocations.", "content": "Analysis of meiotic chromosomes from two human male translocation heterozygotes is reported. One had total sterility after maturation arrest and chain configurations at meiosis, and the second had a abnormal children who were presumed to be genetically unbalanced.", "contents": "Meiosis in two human reciprocal translocations. Analysis of meiotic chromosomes from two human male translocation heterozygotes is reported. One had total sterility after maturation arrest and chain configurations at meiosis, and the second had a abnormal children who were presumed to be genetically unbalanced."}
{"id": "PMID:469889", "title": "Familial partial 14 trisomy.", "content": "Four children in the same family have 47, +der (14), t(9;14) (p24;q21). Their mothers are sisters with 46,XX,t(9;14) (p24;q21). Clinical features of the children are similar to those of others reported to have partial 14 trisomy.", "contents": "Familial partial 14 trisomy. Four children in the same family have 47, +der (14), t(9;14) (p24;q21). Their mothers are sisters with 46,XX,t(9;14) (p24;q21). Clinical features of the children are similar to those of others reported to have partial 14 trisomy."}
{"id": "PMID:469890", "title": "Extra small metacentric chromosome identified as i(18p).", "content": "A case of a supernumerary metacentric small chromosome, diagnosed at birth, is described. The cytogenetic findings support its identification as i(18p). The clinical development from birth to 12 months is reported, with particular attention given to the psychomotor retardation and to the immunological aspect.", "contents": "Extra small metacentric chromosome identified as i(18p). A case of a supernumerary metacentric small chromosome, diagnosed at birth, is described. The cytogenetic findings support its identification as i(18p). The clinical development from birth to 12 months is reported, with particular attention given to the psychomotor retardation and to the immunological aspect."}
{"id": "PMID:469891", "title": "Familial dicentric translocation t(13;18)(p13;p11.2) ascertained by recurrent miscarriages.", "content": "A dicentric translocation is described involving chromosomes 13 and 18 in which the centromere of chromosome 13 was suppressed. The translocation was ascertained by repeated miscarriages and was found in three generations of phenotypically normal carriers.", "contents": "Familial dicentric translocation t(13;18)(p13;p11.2) ascertained by recurrent miscarriages. A dicentric translocation is described involving chromosomes 13 and 18 in which the centromere of chromosome 13 was suppressed. The translocation was ascertained by repeated miscarriages and was found in three generations of phenotypically normal carriers."}
{"id": "PMID:469892", "title": "The 9p-syndrome.", "content": "A 13-year-old boy with 9p- (p22 leads to pter) is reported. He had many features in common with previous 9p- cases, as well as several distinctive features including polydactyly and precocious puberty. Cytogenetic studies revealed a de novo deletion distal to ban 9p22, which was the reported site of chromosome break in 9 of the 10 previous 9p- cases. Evaluation of the human GALT enzyme suggests that its locus is not on the deleted segment.", "contents": "The 9p-syndrome. A 13-year-old boy with 9p- (p22 leads to pter) is reported. He had many features in common with previous 9p- cases, as well as several distinctive features including polydactyly and precocious puberty. Cytogenetic studies revealed a de novo deletion distal to ban 9p22, which was the reported site of chromosome break in 9 of the 10 previous 9p- cases. Evaluation of the human GALT enzyme suggests that its locus is not on the deleted segment."}
{"id": "PMID:469893", "title": "Variation in chromosome 19.", "content": "Variations in centromeric staining of chromosome 19 appear to be an uncommon polymorphism inherited in a Mendelian manner and easily seen in G-banded cells. It should not be misinterpreted as a structural cytogenetic abnormality.", "contents": "Variation in chromosome 19. Variations in centromeric staining of chromosome 19 appear to be an uncommon polymorphism inherited in a Mendelian manner and easily seen in G-banded cells. It should not be misinterpreted as a structural cytogenetic abnormality."}
{"id": "PMID:469894", "title": "Sixteen years' experience of counselling, diagnosis, and prenatal detection in one genetic centre: progress, results, and problems.", "content": "The work of one Genetic Centre over 16 years, covering about 14 000 kinships, is described. The numbers registered in a year increased from an average of 477 in the early 1960s to 1612 in 1976/1977. The increase is largely, but not entirely, attributable to the advent of prenatal diagnosis, and an account is given of our experience with this. In 1916 patients who had a successful amniocentesis, results indicative of fetal abnormality were found in 4.3% and a balanced translocation was found in an additional 0.9%. Results indicative of fetal abnormality were found in 3.5% of mothers referred because of a maternal age of 40 or more, 3.9% referred because of a high risk of neural tube defect, and 19.3% referred because of a high risk of an inborn error of metabolism. A number of cases with difficult diagnostic problems are described.", "contents": "Sixteen years' experience of counselling, diagnosis, and prenatal detection in one genetic centre: progress, results, and problems. The work of one Genetic Centre over 16 years, covering about 14 000 kinships, is described. The numbers registered in a year increased from an average of 477 in the early 1960s to 1612 in 1976/1977. The increase is largely, but not entirely, attributable to the advent of prenatal diagnosis, and an account is given of our experience with this. In 1916 patients who had a successful amniocentesis, results indicative of fetal abnormality were found in 4.3% and a balanced translocation was found in an additional 0.9%. Results indicative of fetal abnormality were found in 3.5% of mothers referred because of a maternal age of 40 or more, 3.9% referred because of a high risk of neural tube defect, and 19.3% referred because of a high risk of an inborn error of metabolism. A number of cases with difficult diagnostic problems are described."}
{"id": "PMID:469896", "title": "Congenital haemolytic anaemia resulting from glucose phosphate isomerase deficiency: genetics, clinical picture, and prenatal diagnosis.", "content": "Glucose phosphate isomerase (GPI) deficiency with severe haemolysis and hydrops fetalis was found in the first child of unrelated, healthy Caucasian parents. The child died at 3 hours. Both parents were found to have 50% of normal red cell GPI activity and qualitative tests on their red cells and white cells showed that each was heterozygous for a different GPI variant allele associated with enzyme deficiency. Tests on the placenta showed that the propositus was a 'compound' heterozygote. Examination of amniotic cells obtained by amniocentesis on the mother at 28 weeks in her second pregnancy led to the prenatal diagnosis of GPI deficiency. This second child, a 'compound' heterozygote at the GPI locus indistinguishable from the first, was successfully treated by immediate exchange transfusion and subsequent blood transfusions.", "contents": "Congenital haemolytic anaemia resulting from glucose phosphate isomerase deficiency: genetics, clinical picture, and prenatal diagnosis. Glucose phosphate isomerase (GPI) deficiency with severe haemolysis and hydrops fetalis was found in the first child of unrelated, healthy Caucasian parents. The child died at 3 hours. Both parents were found to have 50% of normal red cell GPI activity and qualitative tests on their red cells and white cells showed that each was heterozygous for a different GPI variant allele associated with enzyme deficiency. Tests on the placenta showed that the propositus was a 'compound' heterozygote. Examination of amniotic cells obtained by amniocentesis on the mother at 28 weeks in her second pregnancy led to the prenatal diagnosis of GPI deficiency. This second child, a 'compound' heterozygote at the GPI locus indistinguishable from the first, was successfully treated by immediate exchange transfusion and subsequent blood transfusions."}
{"id": "PMID:469897", "title": "Inherited anaemias in the Greek community of Cape Town.", "content": "Cape Town has a Greek community of about 5000, of whom approximately 75% originate from the island of Lesbos. In a survey of inherited haematological conditions in this population, 250 unrelated volunteers were investigated. The prevalence of heterozygous beta-thalassaemia was found to be 6.4%, with a gene frequency of 0.033. G6PD deficiency was detected in 10 males and it can be estimated that the prevalence in the male members of this population is 6.7%, with a gene frequency of 0.067. Hereditary spherocytosis was found in three respondents and this represents a prevalence of 1.2%, with a gene frequency of 0.006. One subject was heterozygous for the sickle cell trait (HbS) and another volunteer had haemoglobin Lepore, which had already been diagnosed in Greece. Our findings with respect to beta-thalassaemia and G6PD deficiency are similar to those reported from regions in Greece where malaria is not highly endemic.", "contents": "Inherited anaemias in the Greek community of Cape Town. Cape Town has a Greek community of about 5000, of whom approximately 75% originate from the island of Lesbos. In a survey of inherited haematological conditions in this population, 250 unrelated volunteers were investigated. The prevalence of heterozygous beta-thalassaemia was found to be 6.4%, with a gene frequency of 0.033. G6PD deficiency was detected in 10 males and it can be estimated that the prevalence in the male members of this population is 6.7%, with a gene frequency of 0.067. Hereditary spherocytosis was found in three respondents and this represents a prevalence of 1.2%, with a gene frequency of 0.006. One subject was heterozygous for the sickle cell trait (HbS) and another volunteer had haemoglobin Lepore, which had already been diagnosed in Greece. Our findings with respect to beta-thalassaemia and G6PD deficiency are similar to those reported from regions in Greece where malaria is not highly endemic."}
{"id": "PMID:469898", "title": "Hepatitis--Bs antigen in an isolated Indian population of southern Venezuela: a family study.", "content": "A genetic analysis of the presence of HBsAg in a population of which 7.2% of the members were positive is presented. Though the ratios of carriers: non-carriers were generally in good agreement with expectation if the carrier state were determined by homozygosity for a single recessive gene, the two examples of mating most critical to a test of the hypothesis, carrier X carrier, yielded 2 normal children among 4 in one family, and one normal child, the only offspring, in the second family. Other investigators have reported similar findings. We conclude that the hypothesis of simple recessive inheritance cannot be sustained.", "contents": "Hepatitis--Bs antigen in an isolated Indian population of southern Venezuela: a family study. A genetic analysis of the presence of HBsAg in a population of which 7.2% of the members were positive is presented. Though the ratios of carriers: non-carriers were generally in good agreement with expectation if the carrier state were determined by homozygosity for a single recessive gene, the two examples of mating most critical to a test of the hypothesis, carrier X carrier, yielded 2 normal children among 4 in one family, and one normal child, the only offspring, in the second family. Other investigators have reported similar findings. We conclude that the hypothesis of simple recessive inheritance cannot be sustained."}
{"id": "PMID:469899", "title": "Studies in mice on the mutagenicity of two contraceptive drugs.", "content": "An experiment is described which tests for visible and invisible mutants in mice treated with four different doses each of the contraceptives Gynanovlar and Lyndiol. The results show that there is no reason to suppose that either substance has an appreciable mutagenic effect, expressed as an increase of antenatal and postnatal lethals or visibles. The substrain CBA/CagCam, used throughout, has an incidence of 0.27% of singly occurring abnormalities, mainly of the appendicular skeleton, which distinguishes it from the parent CBA strain and its axial variation described by Gruneberg (1963).", "contents": "Studies in mice on the mutagenicity of two contraceptive drugs. An experiment is described which tests for visible and invisible mutants in mice treated with four different doses each of the contraceptives Gynanovlar and Lyndiol. The results show that there is no reason to suppose that either substance has an appreciable mutagenic effect, expressed as an increase of antenatal and postnatal lethals or visibles. The substrain CBA/CagCam, used throughout, has an incidence of 0.27% of singly occurring abnormalities, mainly of the appendicular skeleton, which distinguishes it from the parent CBA strain and its axial variation described by Gruneberg (1963)."}
{"id": "PMID:469900", "title": "No increased chromosome breakage in three Bloom's syndrome heterozygotes.", "content": "The frequency of chromosome aberrations in the lymphocytes of three established heterozygotes for the Bloom's syndrome gene (ages 67, 57, 46) was compared to that in controls (ages 68, 67, 61, 46, 34). The main part of the study was done on coded slides. No difference was found between the heterozygotes and the control group, except for one control (aged 46) who had a significantly higher number of chromosome aberrations than the others.", "contents": "No increased chromosome breakage in three Bloom's syndrome heterozygotes. The frequency of chromosome aberrations in the lymphocytes of three established heterozygotes for the Bloom's syndrome gene (ages 67, 57, 46) was compared to that in controls (ages 68, 67, 61, 46, 34). The main part of the study was done on coded slides. No difference was found between the heterozygotes and the control group, except for one control (aged 46) who had a significantly higher number of chromosome aberrations than the others."}
{"id": "PMID:469901", "title": "X-mapping in man: evidence against measurable linkage between anhidrotic ectodermal dysplasia and G6PD deficiency.", "content": "A Sardinian kindred segregating for X-linked anhidrotic ectodermal dysplasia (AED), glucose-6-phosphate dehydrogenase (G6PD) deficiency of Mediterranean type, and Xga blood antigen provides evidence against a measurable linkage between the loci for AED and G6PD. Moreover, from the segregation of the combined phenotypes in four scorable sons from two triple heterozygotes with phase known, it seems highly probable that the AED locus is nearer to the centromere than is the G6PD locus.", "contents": "X-mapping in man: evidence against measurable linkage between anhidrotic ectodermal dysplasia and G6PD deficiency. A Sardinian kindred segregating for X-linked anhidrotic ectodermal dysplasia (AED), glucose-6-phosphate dehydrogenase (G6PD) deficiency of Mediterranean type, and Xga blood antigen provides evidence against a measurable linkage between the loci for AED and G6PD. Moreover, from the segregation of the combined phenotypes in four scorable sons from two triple heterozygotes with phase known, it seems highly probable that the AED locus is nearer to the centromere than is the G6PD locus."}
{"id": "PMID:469902", "title": "Additional pedigree supporting the frequent origin of XXYY from consecutive meiotic non-disjunction in paternal gametogenesis.", "content": "A 48,XXYY Sardinian patient, born to a 46,XX mother heterozygous for the Gd Mediterranean mutant, and to a 46,XY father hemizygous for the same mutant, was found to have a mosaic distribution of G6PD(+) and G6PD(-) peripheral red blood cells. The most likely interpretation of this finding is that the propositus is an additional example of an XXYY zygote derived from a consecutive meiotic non-disjunction during paternal gametogenesis.", "contents": "Additional pedigree supporting the frequent origin of XXYY from consecutive meiotic non-disjunction in paternal gametogenesis. A 48,XXYY Sardinian patient, born to a 46,XX mother heterozygous for the Gd Mediterranean mutant, and to a 46,XY father hemizygous for the same mutant, was found to have a mosaic distribution of G6PD(+) and G6PD(-) peripheral red blood cells. The most likely interpretation of this finding is that the propositus is an additional example of an XXYY zygote derived from a consecutive meiotic non-disjunction during paternal gametogenesis."}
{"id": "PMID:469903", "title": "Trisomy 20p from maternal t(3;20) translocation.", "content": "A case of trisomy 20p resulting from a maternal translocation t(3;20) is described. QM and BUdR banding techniques were used for its identification. A round face with oblique palpebral fissures, strabismus, cardiac and vertebral abnormalities, mild psychomotor retardation, together with poor coordination and speech impediment, are the most typical features of the proband.", "contents": "Trisomy 20p from maternal t(3;20) translocation. A case of trisomy 20p resulting from a maternal translocation t(3;20) is described. QM and BUdR banding techniques were used for its identification. A round face with oblique palpebral fissures, strabismus, cardiac and vertebral abnormalities, mild psychomotor retardation, together with poor coordination and speech impediment, are the most typical features of the proband."}
{"id": "PMID:469904", "title": "Triple mosaicism 45,XY,--18/46, XY/47,XY,+18.", "content": "A patient with symptoms clinically resembling Edwards's syndrome is presented. Cranial asymmetry, thoracic and lumbar hemivertebrae, and an additional rib were the unusual features. The cytogenetic studies revealed the coexistence of three separate cell lines with 45,XY,--18/46,XY/47,XY,+18 complement.", "contents": "Triple mosaicism 45,XY,--18/46, XY/47,XY,+18. A patient with symptoms clinically resembling Edwards's syndrome is presented. Cranial asymmetry, thoracic and lumbar hemivertebrae, and an additional rib were the unusual features. The cytogenetic studies revealed the coexistence of three separate cell lines with 45,XY,--18/46,XY/47,XY,+18 complement."}
{"id": "PMID:469905", "title": "The Prader-Willi syndrome with a 15/3 translocation.", "content": "A de novo translocation of 15q to 3p with complete monosomy of 15p and partial monosomy of 15q was detected by trypsin banding on peripheral lymphocytes of a 5-year-old boy with Prader-Willi syndrome (severe mental retardation, dyslalia, cryptorchidism, and muscular hypotonia). The pathogenic role of chromosome 15 abnormalities in the aetiology of this syndrome is discussed.", "contents": "The Prader-Willi syndrome with a 15/3 translocation. A de novo translocation of 15q to 3p with complete monosomy of 15p and partial monosomy of 15q was detected by trypsin banding on peripheral lymphocytes of a 5-year-old boy with Prader-Willi syndrome (severe mental retardation, dyslalia, cryptorchidism, and muscular hypotonia). The pathogenic role of chromosome 15 abnormalities in the aetiology of this syndrome is discussed."}
{"id": "PMID:469908", "title": "Priorities of nutritional concepts assigned by health professionals and students.", "content": "Medical faculty, practicing physicians, medical students, and dietitians/nutritionists rated nutritional concepts as to their importance for inclusion in the medical school curricula and indicated the best method of accomplishing this. Ten concepts were rated \"essential.\" They related to biochemical and physiological aspects of nutrition; nutrition in pregnancy, lactation, growth, development, maturity, and old age; nutritional management in disease; drug and diet interrelationships; and critiques of popular dietary regimens. Ranked as \"important\" were eight concepts regarding sources of nutrition information; evaluation of nutritional status; recommended dietary allowances; and food additives, naturally occurring food toxins, and nutrient composition of foods. Based on results of this study, the authors make recommendations regarding nutritional concepts that should be included in medical school curricula and how these concepts should be incorporated.", "contents": "Priorities of nutritional concepts assigned by health professionals and students. Medical faculty, practicing physicians, medical students, and dietitians/nutritionists rated nutritional concepts as to their importance for inclusion in the medical school curricula and indicated the best method of accomplishing this. Ten concepts were rated \"essential.\" They related to biochemical and physiological aspects of nutrition; nutrition in pregnancy, lactation, growth, development, maturity, and old age; nutritional management in disease; drug and diet interrelationships; and critiques of popular dietary regimens. Ranked as \"important\" were eight concepts regarding sources of nutrition information; evaluation of nutritional status; recommended dietary allowances; and food additives, naturally occurring food toxins, and nutrient composition of foods. Based on results of this study, the authors make recommendations regarding nutritional concepts that should be included in medical school curricula and how these concepts should be incorporated."}
{"id": "PMID:469909", "title": "The house officer as a teacher: what schools expect and measure.", "content": "Teaching is generally regarded as an important function of the house officer not only from a practical viewpoint but also as an integral part of the training of a professional. A survey by the Association of American Medical Colleges shows, however, that unlike other competencies, the house officer's teaching is not explicitly evaluated in most graduate training programs. Furthermore, it appears that the teaching role itself is not always well defined. It is urged that, if the teaching role is regarded as an important element in graduate medical education, earlier and more gradual preparation of the medical student for that role is needed.", "contents": "The house officer as a teacher: what schools expect and measure. Teaching is generally regarded as an important function of the house officer not only from a practical viewpoint but also as an integral part of the training of a professional. A survey by the Association of American Medical Colleges shows, however, that unlike other competencies, the house officer's teaching is not explicitly evaluated in most graduate training programs. Furthermore, it appears that the teaching role itself is not always well defined. It is urged that, if the teaching role is regarded as an important element in graduate medical education, earlier and more gradual preparation of the medical student for that role is needed."}
{"id": "PMID:469910", "title": "Medical student attitude toward seeking professional psychological help.", "content": "To explore the antecedents of emotional distress among physicians, the authors investigated the relationship between year in medical school and student attitude toward seeking professional psychological help. The sample consisted of 489 students at the University of Southern California School of Medicine. Since a cross-sectional approach was employed a classical regression analysis was used to control for possible differences in the variables of sex, age, contact with psychotherapy, and specialty choice. Differences in male and female responses prompted separate analyses. For women, no attitudinal changes were noted during medical school. For men, a significant positive change in attitude was noted between the students of years one and two, as compared with students of years three and four. Results were contrary to findings in the literature. Several possible explanations are discussed.", "contents": "Medical student attitude toward seeking professional psychological help. To explore the antecedents of emotional distress among physicians, the authors investigated the relationship between year in medical school and student attitude toward seeking professional psychological help. The sample consisted of 489 students at the University of Southern California School of Medicine. Since a cross-sectional approach was employed a classical regression analysis was used to control for possible differences in the variables of sex, age, contact with psychotherapy, and specialty choice. Differences in male and female responses prompted separate analyses. For women, no attitudinal changes were noted during medical school. For men, a significant positive change in attitude was noted between the students of years one and two, as compared with students of years three and four. Results were contrary to findings in the literature. Several possible explanations are discussed."}
{"id": "PMID:469911", "title": "Teacher-student interaction in a medical clerkship.", "content": "Seventeen randomly selected clinical teaching sessions in a medical school clerkship were videotaped during a one-month period, including teaching rounds, working rounds, morning report sessions, lectures, patient management conferences, grand rounds, and journal clubs. Using a verbal behavior classification schedule, the investigators analyzed the videotapes in terms of the proportion of talking done by clerkship instructors, medical students, residents, and others. The nature of the verbal interaction was examined by assessing the proportion of time devoted to giving information versus asking questions. The proportions on these dimensions were further analyzed according to the cognitive level of the verbal interaction. The data suggest that the teaching observed was not optimal for promoting problem-solving ability, since students were placed in a very passive role in which they received a preponderance of low-level, factual information.", "contents": "Teacher-student interaction in a medical clerkship. Seventeen randomly selected clinical teaching sessions in a medical school clerkship were videotaped during a one-month period, including teaching rounds, working rounds, morning report sessions, lectures, patient management conferences, grand rounds, and journal clubs. Using a verbal behavior classification schedule, the investigators analyzed the videotapes in terms of the proportion of talking done by clerkship instructors, medical students, residents, and others. The nature of the verbal interaction was examined by assessing the proportion of time devoted to giving information versus asking questions. The proportions on these dimensions were further analyzed according to the cognitive level of the verbal interaction. The data suggest that the teaching observed was not optimal for promoting problem-solving ability, since students were placed in a very passive role in which they received a preponderance of low-level, factual information."}
{"id": "PMID:469912", "title": "The magnitude of declining psychiatric career choice.", "content": "Previous studies are reviewed and new data are reported which document a decline in the percentage of American medical graduates choosing psychiatric careers. Data on absolute numbers are conflicting. While 7 percent of graduates from 1945 to 1964 and 6.4 percent of graduates from 1965 to 1970 chose psychiatry, only 5.3 percent of graduates of the classes of 1975 and 1976 did so. Available data for more recent medical classes indicate that further decrements will occur. Possible explanations and strategies for research are briefly outlines.", "contents": "The magnitude of declining psychiatric career choice. Previous studies are reviewed and new data are reported which document a decline in the percentage of American medical graduates choosing psychiatric careers. Data on absolute numbers are conflicting. While 7 percent of graduates from 1945 to 1964 and 6.4 percent of graduates from 1965 to 1970 chose psychiatry, only 5.3 percent of graduates of the classes of 1975 and 1976 did so. Available data for more recent medical classes indicate that further decrements will occur. Possible explanations and strategies for research are briefly outlines."}
{"id": "PMID:469913", "title": "Reducing ward-clinic conflicts by rotational scheduling of house staff.", "content": "Many internal medicine residency training programs are being modified to permit each resident to gain more outpatient experience, including providing continuous care to a defined panel of patients for three years. Increasing emphasis on these outpatient responsibilities and education, however, places demands on the resident that conflict with his responsibilities on the inpatient wards. In this paper the authors describe the development and implementation of a rotational scheduling system aimed at substantially alleviating the conflict between inpatient and outpatient responsibilities. During the first year of operation 84 percent of house staff members indicated their preference for continuing the system in future years.", "contents": "Reducing ward-clinic conflicts by rotational scheduling of house staff. Many internal medicine residency training programs are being modified to permit each resident to gain more outpatient experience, including providing continuous care to a defined panel of patients for three years. Increasing emphasis on these outpatient responsibilities and education, however, places demands on the resident that conflict with his responsibilities on the inpatient wards. In this paper the authors describe the development and implementation of a rotational scheduling system aimed at substantially alleviating the conflict between inpatient and outpatient responsibilities. During the first year of operation 84 percent of house staff members indicated their preference for continuing the system in future years."}
{"id": "PMID:469914", "title": "Teaching medical gerontology: utilization of a psychiatry consultation program.", "content": "This is a report of a pilot project designed to explore the feasibility of using the clinical Adult Service Psychiatry Consultation-Liaison Program at the University of Michigan as the locus for teaching medical gerontology to psychiatric and medical/surgical house officers and medical students. The goals, general techniques, educational content, and implementation procedures are described. Evidence was obtained that teaching medical gerontology through the program is both feasible and effective. Since a nonphysician gerontologist implemented the clinically based teaching program, the project is an example of the possibilities inherent in a multidisciplinary clinical teaching program.", "contents": "Teaching medical gerontology: utilization of a psychiatry consultation program. This is a report of a pilot project designed to explore the feasibility of using the clinical Adult Service Psychiatry Consultation-Liaison Program at the University of Michigan as the locus for teaching medical gerontology to psychiatric and medical/surgical house officers and medical students. The goals, general techniques, educational content, and implementation procedures are described. Evidence was obtained that teaching medical gerontology through the program is both feasible and effective. Since a nonphysician gerontologist implemented the clinically based teaching program, the project is an example of the possibilities inherent in a multidisciplinary clinical teaching program."}
{"id": "PMID:469929", "title": "Passive protection by human serum in mice infected with encapsulated Staphylococcus aureus.", "content": "The occurrence and nature of passive protective antibody in 100 samples of human serum was investigated in mice challenged with strains of Staphylococcus aureus capsular types A (Smith diffuse strain) and B (strain NS58D). Sixty of the sera passively protected mice against the capsular type-A strain, three against type B, and one against both types. Rabbit antisera against human IgG, IgA and IgM could remove the protective activity from a human serum of high potency, and the activity was also sensitive to 2-mercaptoethanol. Absorption with Smith surface antigen removed protective activity and reduced the concentration of IgG 7-fold, IgA 2.7 fold and of IgM 3-fold more than in a non-protective serum. Consequently, the protective activity of human serum is believed to be associated with antibodies to the S. aureus capsular antigen in the three immunoglobulin classes.", "contents": "Passive protection by human serum in mice infected with encapsulated Staphylococcus aureus. The occurrence and nature of passive protective antibody in 100 samples of human serum was investigated in mice challenged with strains of Staphylococcus aureus capsular types A (Smith diffuse strain) and B (strain NS58D). Sixty of the sera passively protected mice against the capsular type-A strain, three against type B, and one against both types. Rabbit antisera against human IgG, IgA and IgM could remove the protective activity from a human serum of high potency, and the activity was also sensitive to 2-mercaptoethanol. Absorption with Smith surface antigen removed protective activity and reduced the concentration of IgG 7-fold, IgA 2.7 fold and of IgM 3-fold more than in a non-protective serum. Consequently, the protective activity of human serum is believed to be associated with antibodies to the S. aureus capsular antigen in the three immunoglobulin classes."}
{"id": "PMID:469930", "title": "Inhibition by amoeba-specific antiserum and by cytochalasin B of the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures.", "content": "Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of Naegleria fowleri cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant contact with the ME cells. Mammalian-cell damage occurred only when the organisms recovered pseudopodium function and began to migrate over the monolayers as they overcame the inhibitory effects of the antiserum. Cytochalasin B at a concentration of 10 microgram/ml, shown to prevent the engulfment of chick erythrocytes by amoebae, also inhibited the cytopathogenicity of Naegleria when incorporated in ME-cell culture medium. Despite repeated contact with active trophozoites, the ME cells showed only those morphological changes characteristically induced by cytochalasin B itself. The amoebae in turn showed signs of starvation after 3 or 4 days' incubation, suggesting that the feeding activity of trophozoites was suppressed. Colchicine, on the other hand, inhibited neither the ingestion of erythrocytes nor the destruction of ME cells by amoebae. It was concluded that the cytopathogenicity of N. fowleri in ME-cell cultures was due to physical rather than biochemical or cytotoxic mechanisms and was associated with the phagocytic activity of trophozoites.", "contents": "Inhibition by amoeba-specific antiserum and by cytochalasin B of the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures. Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of Naegleria fowleri cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant contact with the ME cells. Mammalian-cell damage occurred only when the organisms recovered pseudopodium function and began to migrate over the monolayers as they overcame the inhibitory effects of the antiserum. Cytochalasin B at a concentration of 10 microgram/ml, shown to prevent the engulfment of chick erythrocytes by amoebae, also inhibited the cytopathogenicity of Naegleria when incorporated in ME-cell culture medium. Despite repeated contact with active trophozoites, the ME cells showed only those morphological changes characteristically induced by cytochalasin B itself. The amoebae in turn showed signs of starvation after 3 or 4 days' incubation, suggesting that the feeding activity of trophozoites was suppressed. Colchicine, on the other hand, inhibited neither the ingestion of erythrocytes nor the destruction of ME cells by amoebae. It was concluded that the cytopathogenicity of N. fowleri in ME-cell cultures was due to physical rather than biochemical or cytotoxic mechanisms and was associated with the phagocytic activity of trophozoites."}
{"id": "PMID:469931", "title": "Adherence of Enterobacteriaceae to human buccal cells.", "content": "A preliminary examination has been made of the adherencae isolated from sputa. The radioadherence method was found to correlate well with the conventional light-microscope adherence technique. Saturation of buccal-cell binding sites with bacteria occurred when less than 10% of the buccal-cell surface was occupied. The adherence of Enterobacter aerogenes to buccal cells was impaired by the prior adherence of bacilli of either the same strain, or of a strain of Klebsiella pneumoniae.", "contents": "Adherence of Enterobacteriaceae to human buccal cells. A preliminary examination has been made of the adherencae isolated from sputa. The radioadherence method was found to correlate well with the conventional light-microscope adherence technique. Saturation of buccal-cell binding sites with bacteria occurred when less than 10% of the buccal-cell surface was occupied. The adherence of Enterobacter aerogenes to buccal cells was impaired by the prior adherence of bacilli of either the same strain, or of a strain of Klebsiella pneumoniae."}
{"id": "PMID:469932", "title": "Electron microscopy of Campylobacter jejuni.", "content": "Stools from 56 patients with gastroenteritis were cultured for Campylobacter jejuni. The five strains isolated were examined by electron microscopy. The campylobacter cells were pleomorphic and most displayed appearances similar to those of V. fetus. Morphological changes were observed in cultures subjected to prolonged incubation.", "contents": "Electron microscopy of Campylobacter jejuni. Stools from 56 patients with gastroenteritis were cultured for Campylobacter jejuni. The five strains isolated were examined by electron microscopy. The campylobacter cells were pleomorphic and most displayed appearances similar to those of V. fetus. Morphological changes were observed in cultures subjected to prolonged incubation."}
{"id": "PMID:469934", "title": "A mathematical model of proximal tubule absorption.", "content": "A previous model of the mechanisms of flow through epithelia was modified and extended to include hydrostatic and osmotic pressures in the cells and in the peritubular capillaries. The differential equations for flow and concentration in each region of the proximal tubule were derived. The equations were solved numerically by a finite difference method. The principal conclusions are: (i) Cell NaCl concentration remains essentially isotonic over the pressure variations considered; (ii) channel NaCl concentration varies only a few mosmol from isotonicity, and the hydrostatic and osmotic pressure differences across the cell wall are of the same order of magnitude; (iii) both reabsorbate osmolality and pressure-induced flow are relatively insensitive to the geometry of the system; (iv) a strong equilibrating mechanism exists in the sensitivity of the reabsorbate osmolality to luminal osmolality; this mechanism is far more significant than any other parameter change.", "contents": "A mathematical model of proximal tubule absorption. A previous model of the mechanisms of flow through epithelia was modified and extended to include hydrostatic and osmotic pressures in the cells and in the peritubular capillaries. The differential equations for flow and concentration in each region of the proximal tubule were derived. The equations were solved numerically by a finite difference method. The principal conclusions are: (i) Cell NaCl concentration remains essentially isotonic over the pressure variations considered; (ii) channel NaCl concentration varies only a few mosmol from isotonicity, and the hydrostatic and osmotic pressure differences across the cell wall are of the same order of magnitude; (iii) both reabsorbate osmolality and pressure-induced flow are relatively insensitive to the geometry of the system; (iv) a strong equilibrating mechanism exists in the sensitivity of the reabsorbate osmolality to luminal osmolality; this mechanism is far more significant than any other parameter change."}
{"id": "PMID:469947", "title": "Glycogen metabolism in the prelaid chick embryo.", "content": "Glycogen metabolism has been studied during the development of the early chick embryo, at the cytochemical and ultrastructural levels. Two waves of glycogen synthesis and breakdown have been found. In the first, free clusters of glycogen particles are synthesized at late oogenesis. These clusters are found later in invaginations of the membrane of vesicles containing a floccular material (FLOV). The glycogen clusters are degraded there during ovulation and the first hours in the oviduct. The second wave of glycogen synthesis begins before cleavage, reaching a maximum at mid-uterine age. This second wave occurs in another type of vesicle (GLYV), which eventually disintegrates releasing free clusters of glycogen granules. This glycogen is degraded in membranous structures containing a floccular material, as in the first wave of degradation. The degradation ends at the late uterine stages, and at the same time numerous ribosomes are formed. This period corresponds to area pellucida formation, which probably depends on the energy liberated during the second wave of glycogen degradation.", "contents": "Glycogen metabolism in the prelaid chick embryo. Glycogen metabolism has been studied during the development of the early chick embryo, at the cytochemical and ultrastructural levels. Two waves of glycogen synthesis and breakdown have been found. In the first, free clusters of glycogen particles are synthesized at late oogenesis. These clusters are found later in invaginations of the membrane of vesicles containing a floccular material (FLOV). The glycogen clusters are degraded there during ovulation and the first hours in the oviduct. The second wave of glycogen synthesis begins before cleavage, reaching a maximum at mid-uterine age. This second wave occurs in another type of vesicle (GLYV), which eventually disintegrates releasing free clusters of glycogen granules. This glycogen is degraded in membranous structures containing a floccular material, as in the first wave of degradation. The degradation ends at the late uterine stages, and at the same time numerous ribosomes are formed. This period corresponds to area pellucida formation, which probably depends on the energy liberated during the second wave of glycogen degradation."}
{"id": "PMID:469949", "title": "Environmental chemical carcinogens and liver cancer.", "content": "An appraisal is made of these chemical carcinogens available in the human environment that have been implicated in the etiology of liver cancer. The possible role of mycotoxins is discussed in detail, in particular the association between the aflatoxins and liver cancer in Africa and the Far East.", "contents": "Environmental chemical carcinogens and liver cancer. An appraisal is made of these chemical carcinogens available in the human environment that have been implicated in the etiology of liver cancer. The possible role of mycotoxins is discussed in detail, in particular the association between the aflatoxins and liver cancer in Africa and the Far East."}
{"id": "PMID:469955", "title": "Distribution of glutamate in layers of the rabbit hippocampal fields CA1, CA3, and the dentate area.", "content": "The distribution of L-glutamic acid in single layers dissected from freeze-dried sections of the rabbit hippocampus was estimated by means of an enzymatic cycling procedure. Although the regional variation in glutamate content is less than that of other transmitter candidates, there were present significant differences in it between the projection fields of certain fiber connections. Glutamate concentration was highest in the termination areas of commissural fibers and Schaffer's collaterals.", "contents": "Distribution of glutamate in layers of the rabbit hippocampal fields CA1, CA3, and the dentate area. The distribution of L-glutamic acid in single layers dissected from freeze-dried sections of the rabbit hippocampus was estimated by means of an enzymatic cycling procedure. Although the regional variation in glutamate content is less than that of other transmitter candidates, there were present significant differences in it between the projection fields of certain fiber connections. Glutamate concentration was highest in the termination areas of commissural fibers and Schaffer's collaterals."}
{"id": "PMID:469950", "title": "Role of pesticides in hepatocarcinogenesis.", "content": "Pesticides are biologically active substances that are significant environmental contaminants. Only a few of the many pesticides have been subjected to short- or long-term carcinogenicity tests. To date, 16 of them have been identified as hepatocarcinogenic agents in animals, usually mice. However, their cancer-inducing effect in humans has not been completely proved. The use of short-term screening tests (first of all the Salmonella-microsome test) for routine screening of pesticides on a large scale is highly recommended. The herbicide 2,4,5-trichlorophenoxyethanol (TCPE) containing different amounts of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was tested for carcinogenicity in Swiss mice. TCPE enhanced liver tumors in males but TCDD did not affect liver tumor incidence. The occurrence of liver tumors was shown to be dose-dependent. There was no correlation between the results of in vivo carcinogenicity studies and the induction of aryl hydrocarbon hydroxylase and biphenyl 2-hydroxylase activities.", "contents": "Role of pesticides in hepatocarcinogenesis. Pesticides are biologically active substances that are significant environmental contaminants. Only a few of the many pesticides have been subjected to short- or long-term carcinogenicity tests. To date, 16 of them have been identified as hepatocarcinogenic agents in animals, usually mice. However, their cancer-inducing effect in humans has not been completely proved. The use of short-term screening tests (first of all the Salmonella-microsome test) for routine screening of pesticides on a large scale is highly recommended. The herbicide 2,4,5-trichlorophenoxyethanol (TCPE) containing different amounts of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was tested for carcinogenicity in Swiss mice. TCPE enhanced liver tumors in males but TCDD did not affect liver tumor incidence. The occurrence of liver tumors was shown to be dose-dependent. There was no correlation between the results of in vivo carcinogenicity studies and the induction of aryl hydrocarbon hydroxylase and biphenyl 2-hydroxylase activities."}
{"id": "PMID:469951", "title": "Promotion of liver tumors by steroid hormones.", "content": "Corticosterone inhibits proliferation of rat liver cells, but it can be inactivated by blocking its cytoplasmic receptors in liver cells with other steroids (progesterone). High levels of progesterones in animals lead to stimulated proliferation in the liver. Abnormally high proliferation in the liver may promote malignant transformation or may make it possible for smaller amounts of hepatocarcinogenic substances to induce tumors in a shorter time than normal.", "contents": "Promotion of liver tumors by steroid hormones. Corticosterone inhibits proliferation of rat liver cells, but it can be inactivated by blocking its cytoplasmic receptors in liver cells with other steroids (progesterone). High levels of progesterones in animals lead to stimulated proliferation in the liver. Abnormally high proliferation in the liver may promote malignant transformation or may make it possible for smaller amounts of hepatocarcinogenic substances to induce tumors in a shorter time than normal."}
{"id": "PMID:469956", "title": "Mass spectrometric identification of Cu, Zn, Fe, Co, Mn, Mg, and Pb in mammalian brain.", "content": "Combining the techniques of thin-layer chromatography (TLC) and mass spectrometry, we unambiguously identified the trace metals Cu, Zn, Fe, Pb, Mn, Co, and Mg in the brain of a female human who had no evidence of any pathologic disease in the central nervous system, and in brains from mouse, rat, guinea pig, and rabbit. These trace metals were also found in anatomic regions of human brain: cortex (gray), cortex (white), caudate nucleus, putamen, hippocampus, and thalamus, and in anatomic regions of rat brain: hypothalamus, cerebellum, stem striatum, and \"the rest.\" The metals were characterized from the color and Rf values of their tetraphenylporphyrin chelates on TLC and from the mass and pattern of molecule ion cluster of the mass spectrum. The unexpected presence of lead in the brain is discussed.", "contents": "Mass spectrometric identification of Cu, Zn, Fe, Co, Mn, Mg, and Pb in mammalian brain. Combining the techniques of thin-layer chromatography (TLC) and mass spectrometry, we unambiguously identified the trace metals Cu, Zn, Fe, Pb, Mn, Co, and Mg in the brain of a female human who had no evidence of any pathologic disease in the central nervous system, and in brains from mouse, rat, guinea pig, and rabbit. These trace metals were also found in anatomic regions of human brain: cortex (gray), cortex (white), caudate nucleus, putamen, hippocampus, and thalamus, and in anatomic regions of rat brain: hypothalamus, cerebellum, stem striatum, and \"the rest.\" The metals were characterized from the color and Rf values of their tetraphenylporphyrin chelates on TLC and from the mass and pattern of molecule ion cluster of the mass spectrum. The unexpected presence of lead in the brain is discussed."}
{"id": "PMID:469957", "title": "Soluble protein and peptide pattern from different regions of rat brain during pre- and early postnatal development.", "content": "Isotachophoretic pattern from phylogenetically different regions of rat brain during pre- and early postnatal development were studied. Four main observations were made: a) The patterns were greatly similar during maturation of the respective regions studied; b) One complex of peaks disappeared or decreased in quantity during the 2nd and 3rd weeks of postnatal development; c) New peaks appeared and accumulated during the same period; d) Slight differences were observable in the different regions studied.", "contents": "Soluble protein and peptide pattern from different regions of rat brain during pre- and early postnatal development. Isotachophoretic pattern from phylogenetically different regions of rat brain during pre- and early postnatal development were studied. Four main observations were made: a) The patterns were greatly similar during maturation of the respective regions studied; b) One complex of peaks disappeared or decreased in quantity during the 2nd and 3rd weeks of postnatal development; c) New peaks appeared and accumulated during the same period; d) Slight differences were observable in the different regions studied."}
{"id": "PMID:469953", "title": "Importance of hepatic neoplasms in lower vertebrate animals as a tool in cancer research.", "content": "Hepatic tumors were induced in medakas (Oryzias latipes) by the addition of diethylnitrosamine (DEN) to their aquarium water. Medakas are useful for study because they are highly susceptible to the carcinogenic effect of DEN and the time for tumor induction is relatively short. Tumors had a histological pattern similar to that of mammalian hepatic tumors. The medaka seems to be susceptible to water-soluble carcinogens.", "contents": "Importance of hepatic neoplasms in lower vertebrate animals as a tool in cancer research. Hepatic tumors were induced in medakas (Oryzias latipes) by the addition of diethylnitrosamine (DEN) to their aquarium water. Medakas are useful for study because they are highly susceptible to the carcinogenic effect of DEN and the time for tumor induction is relatively short. Tumors had a histological pattern similar to that of mammalian hepatic tumors. The medaka seems to be susceptible to water-soluble carcinogens."}
{"id": "PMID:469958", "title": "Relative normophagia and organ growth in growth-retarded weanling rats with dorsomedial hypothalamic lesions.", "content": "Weanling rats with ventromedial (VMNL) and dorsomedial (DMNL) hypothalamic lesions and sham-operated controls were maintained up to 198 days after operation. Food intake was measured throughout the experiment and organ weights were recorded at various periods of sacrifice. Comparisons were made between controls and VMNL and DMNL rats, respectively. Food intake and organ weights were expressed in absolute terms and relative to body mass and Kleiber's \"metabolic size.\" VMNL rats were always normophagic and showed lower organ weights, regardless of manner of computation. Rats with DMNL, on the other hand, were absolutely hypophagic but relatively normophagic for considerable periods of time when food intake was referred to body mass. A similar relationship obtained for organ weights. The data fit well with previous results and with a hypothesis that holds that DMNL bring about a \"resetting\" of some central nervous control system that not only allows the rat so operated to subsist on lower substrate levels but also regulates normal growth in relation to body mass.", "contents": "Relative normophagia and organ growth in growth-retarded weanling rats with dorsomedial hypothalamic lesions. Weanling rats with ventromedial (VMNL) and dorsomedial (DMNL) hypothalamic lesions and sham-operated controls were maintained up to 198 days after operation. Food intake was measured throughout the experiment and organ weights were recorded at various periods of sacrifice. Comparisons were made between controls and VMNL and DMNL rats, respectively. Food intake and organ weights were expressed in absolute terms and relative to body mass and Kleiber's \"metabolic size.\" VMNL rats were always normophagic and showed lower organ weights, regardless of manner of computation. Rats with DMNL, on the other hand, were absolutely hypophagic but relatively normophagic for considerable periods of time when food intake was referred to body mass. A similar relationship obtained for organ weights. The data fit well with previous results and with a hypothesis that holds that DMNL bring about a \"resetting\" of some central nervous control system that not only allows the rat so operated to subsist on lower substrate levels but also regulates normal growth in relation to body mass."}
{"id": "PMID:469954", "title": "Effect of steroid hormones on liver cells in culture.", "content": "A variety of liver and hepatoma cell lines were incubated in the presence of corticosterone. DNA synthesis was inhibited in some but not all cell lines. One of them, the Fu 5a clone of the Reuber hepatoma cell line, was subjected to corticosterone for 48 h. Incorporation of labeled thymidine into DNA was inhibited up to 10%. Afterward, corticosterone was removed. DNA synthesis then increased 16-fold relative to controls. It was concluded that corticosterone blocks these cells in late G1 phase of the cell cycle and thus leads to synchronization.", "contents": "Effect of steroid hormones on liver cells in culture. A variety of liver and hepatoma cell lines were incubated in the presence of corticosterone. DNA synthesis was inhibited in some but not all cell lines. One of them, the Fu 5a clone of the Reuber hepatoma cell line, was subjected to corticosterone for 48 h. Incorporation of labeled thymidine into DNA was inhibited up to 10%. Afterward, corticosterone was removed. DNA synthesis then increased 16-fold relative to controls. It was concluded that corticosterone blocks these cells in late G1 phase of the cell cycle and thus leads to synchronization."}
{"id": "PMID:469959", "title": "Mesencephalic lesions in female rats resulting in normophagia and reduced body weight.", "content": "The effects of bilateral mescencephalic lesions in the area of the ventral noradrenergic bundle (VNA) were studied in immature and mature female rats. The food intake consumption of the immature lesioned rats did not differ from sham operated controls whether fed a chow or high fat diet. However, after the surgery the body weights of the two groups began to diverge with the immature lesioned rats obtaining a significantly lower body weight. By the end of the study the immature lesioned rats were also significantly shorter than the controls. Both groups showed normal body composition throughout the measurement period. Upon refeeding after a one day fast the immature female rats defended their lower than normal pre-fast body weight. These data are in good agreement with our earlier findings using immature male rats. The mature lesioned animals also showed normal food consumption when fed a chow type diet. However the lesioned animals did show a brief hyperphagia when placed on a high fat diet. Unlike the immature lesioned rats the body weight of these lesioned animals did not differ from their sham operated controls. It is suggested that lesions in the area of the VNA may result in hyperphagia or, in no effects on food intake or body weight as reported by others or normophagia with reduced body weight. Exact lesion placement may be responsible for these divergent findings.", "contents": "Mesencephalic lesions in female rats resulting in normophagia and reduced body weight. The effects of bilateral mescencephalic lesions in the area of the ventral noradrenergic bundle (VNA) were studied in immature and mature female rats. The food intake consumption of the immature lesioned rats did not differ from sham operated controls whether fed a chow or high fat diet. However, after the surgery the body weights of the two groups began to diverge with the immature lesioned rats obtaining a significantly lower body weight. By the end of the study the immature lesioned rats were also significantly shorter than the controls. Both groups showed normal body composition throughout the measurement period. Upon refeeding after a one day fast the immature female rats defended their lower than normal pre-fast body weight. These data are in good agreement with our earlier findings using immature male rats. The mature lesioned animals also showed normal food consumption when fed a chow type diet. However the lesioned animals did show a brief hyperphagia when placed on a high fat diet. Unlike the immature lesioned rats the body weight of these lesioned animals did not differ from their sham operated controls. It is suggested that lesions in the area of the VNA may result in hyperphagia or, in no effects on food intake or body weight as reported by others or normophagia with reduced body weight. Exact lesion placement may be responsible for these divergent findings."}
{"id": "PMID:469960", "title": "Effects of the lateral septum and latent inhibition on successive discrimination learning.", "content": "Damage to the lateral nucleus of the septum in the rat resulted in atypical learning to a previously meaningless stimulus. Nonreinforced preexposure to a stimulus normally makes more difficult subsequent use of that stimulus for learning. The effect of this preexposure on learning is termed latent inhibition and was demonstrated in sham operated animals. Rats in which the lateral septum was damaged demonstrated a paradoxically smaller learning deficit in the presence of a conditioned stimulus that was previously presented without reinforcement, when compared to the performance of both septal lesioned rats no receiving nonreinforced preexposure and sham operates receiving preexposure. The interaction of nonreinforced preexposure and lateral septal lesions can be explained using a proposed sensitization hypothesis.", "contents": "Effects of the lateral septum and latent inhibition on successive discrimination learning. Damage to the lateral nucleus of the septum in the rat resulted in atypical learning to a previously meaningless stimulus. Nonreinforced preexposure to a stimulus normally makes more difficult subsequent use of that stimulus for learning. The effect of this preexposure on learning is termed latent inhibition and was demonstrated in sham operated animals. Rats in which the lateral septum was damaged demonstrated a paradoxically smaller learning deficit in the presence of a conditioned stimulus that was previously presented without reinforcement, when compared to the performance of both septal lesioned rats no receiving nonreinforced preexposure and sham operates receiving preexposure. The interaction of nonreinforced preexposure and lateral septal lesions can be explained using a proposed sensitization hypothesis."}
{"id": "PMID:469961", "title": "Effects of anoxia and depolarization on the movement of carbon atoms derived from glucose into macromolecular fractions in rat brain slices.", "content": "Incorporation of U-14C-glucose into macromolecules (lipid, protein and nucleic acid fractions) of rat brain cortex slices was studied in vitro under conditions of anoxia and reoxygenation. Additionally, the influence of depolarization on control and postanoxic U-14C-glucose metabolism was investigated. Postassium-induced depolarization of the slices lowered their capacity to incorporate 14 from U-14C glucose into proteins and nucleic acids without any changes in the labeling the lipids. Fifteen and 30 minutes of anoxia depressed the rate of 14C incorporation into each of the above macromolecules was partly restored compared to the control. Excess of potassium in the medium during the reoxygenation period inhibited restoration of the synthetic capacity of the slices execpt lipids, into which incorporation of 14C was even stimulated under depolarizing conditions. The influence of anoxia and depolarization were investigated also in different classes of lipids and proteins. 14C incorporation into SDS-extractable and residual proteins and phospholipid fraction containing phosphoinositol was closest to the control during reoxygenation which suggests the relatively highest resistance of these fractions of anoxia.", "contents": "Effects of anoxia and depolarization on the movement of carbon atoms derived from glucose into macromolecular fractions in rat brain slices. Incorporation of U-14C-glucose into macromolecules (lipid, protein and nucleic acid fractions) of rat brain cortex slices was studied in vitro under conditions of anoxia and reoxygenation. Additionally, the influence of depolarization on control and postanoxic U-14C-glucose metabolism was investigated. Postassium-induced depolarization of the slices lowered their capacity to incorporate 14 from U-14C glucose into proteins and nucleic acids without any changes in the labeling the lipids. Fifteen and 30 minutes of anoxia depressed the rate of 14C incorporation into each of the above macromolecules was partly restored compared to the control. Excess of potassium in the medium during the reoxygenation period inhibited restoration of the synthetic capacity of the slices execpt lipids, into which incorporation of 14C was even stimulated under depolarizing conditions. The influence of anoxia and depolarization were investigated also in different classes of lipids and proteins. 14C incorporation into SDS-extractable and residual proteins and phospholipid fraction containing phosphoinositol was closest to the control during reoxygenation which suggests the relatively highest resistance of these fractions of anoxia."}
{"id": "PMID:469962", "title": "High-energy phosphate compounds and some glycolytic substrates in the rat brain during hypoxia.", "content": "The influence of acute and prolonged (with resuscitation) hypoxia, upon some glycolytic substrates and high energy phosphate compounds in the rat brain, was studies. Acute hypoxia decreased the content of glucose and glucose-6-phosphate as well as of high energy phosphate compounds. Stimultaneously, an increase of lactic level was recorded. Some adaptation features could be observed, when 30-minute hypoxia with resuscitation was studied. Brain content of high energy compounds returned to the normal value, while that of glucose increased twice over control. However, the level of lactic acid was still increased.", "contents": "High-energy phosphate compounds and some glycolytic substrates in the rat brain during hypoxia. The influence of acute and prolonged (with resuscitation) hypoxia, upon some glycolytic substrates and high energy phosphate compounds in the rat brain, was studies. Acute hypoxia decreased the content of glucose and glucose-6-phosphate as well as of high energy phosphate compounds. Stimultaneously, an increase of lactic level was recorded. Some adaptation features could be observed, when 30-minute hypoxia with resuscitation was studied. Brain content of high energy compounds returned to the normal value, while that of glucose increased twice over control. However, the level of lactic acid was still increased."}
{"id": "PMID:469963", "title": "A model of GABA transport by cortical synaptosomes from the Long-Evans rat.", "content": "The initial velocity of uptake of GABA by cortical synaptosomes from the Long-Evans rat has been measured as a function of both sodium and GABA concentration. These data were then fitted to the rate equation for each of several possible models, and the models giving least error were identified. Although one cannot unequivocally distinguish between two of the models on the basis of the goodness of fit, deductions based on the fits of subsets of the models point to one of the two models, The major predictions from this model are as follows: 1) Uptake of GABA is totally sodium-dependent. 2) Although plots of 1/v versus 1/[Na]2 are nonlinear, the coupling ratio for transport (Na/GABA) is 2. 3) For transport to take place, the order of combination with carrier must be Na, Na, GABA. 4) Maximal velocity will occur only at infinite Na and GABA concentrations. 5) There is a sigmoidal relationship between apparent maximal velocity (Va) and [Na]. 6) Kt, the [GABA] that gives a velocity equal to Va/2, rises and then falls as [Na] is increased from zero, passing through a maximum at 33.52 mM [Na]. 7) The relationship between initial velocity and [Na] is sigmoidal. 8) Jm, rate of uptake with infinite [Na], is hyperbolically related to [GABA]; Jm approaches Vmax as [GABA] becomes very large. 9) kappa Na, the [Na] giving a velocity equal to Jm/2, declines rapidly from 10(-7) M to 10(-5)M GABA, but is essentially constant at 10(-4)M above. 10) One GABA molecule is translocated per carrier molecule. The model is consistent with observations in the literature concerning the effects of sodium on the kinetics of transport.", "contents": "A model of GABA transport by cortical synaptosomes from the Long-Evans rat. The initial velocity of uptake of GABA by cortical synaptosomes from the Long-Evans rat has been measured as a function of both sodium and GABA concentration. These data were then fitted to the rate equation for each of several possible models, and the models giving least error were identified. Although one cannot unequivocally distinguish between two of the models on the basis of the goodness of fit, deductions based on the fits of subsets of the models point to one of the two models, The major predictions from this model are as follows: 1) Uptake of GABA is totally sodium-dependent. 2) Although plots of 1/v versus 1/[Na]2 are nonlinear, the coupling ratio for transport (Na/GABA) is 2. 3) For transport to take place, the order of combination with carrier must be Na, Na, GABA. 4) Maximal velocity will occur only at infinite Na and GABA concentrations. 5) There is a sigmoidal relationship between apparent maximal velocity (Va) and [Na]. 6) Kt, the [GABA] that gives a velocity equal to Va/2, rises and then falls as [Na] is increased from zero, passing through a maximum at 33.52 mM [Na]. 7) The relationship between initial velocity and [Na] is sigmoidal. 8) Jm, rate of uptake with infinite [Na], is hyperbolically related to [GABA]; Jm approaches Vmax as [GABA] becomes very large. 9) kappa Na, the [Na] giving a velocity equal to Jm/2, declines rapidly from 10(-7) M to 10(-5)M GABA, but is essentially constant at 10(-4)M above. 10) One GABA molecule is translocated per carrier molecule. The model is consistent with observations in the literature concerning the effects of sodium on the kinetics of transport."}
{"id": "PMID:469964", "title": "Cerebral amino acid levels and uptake in rats after portocaval anastomosis: I. Regional studies in vitro.", "content": "Uptake of various amino acids was studied in slices from brain regions of rats four weeks after portocaval anastomosis. No differences of the inulin compartment were observed between control and experimental animals. After 60-minutes incubation, uptake showed an overall pattern of diminution. This was more evident for some amino acids: valine, methionine, and lysine exhibited a lowering of about 30%, which was fairly uniform in the four tested regions; others showed a regional decrease-- alanine in pons-medulla, phenylalanine in cerebellum, histidine and GABA in mesodiencephalon. This decrease did not seen to be related to transport classes. The restricted entry of amino acids into brain cells in portocaval encephalopathy is somewhat difficult to explain; a decreased rate of protein synthesis may be of some importance, but other factors, such as a \"carrier\" impairment, effects on release and on amino acid metabolism, may also be involved.", "contents": "Cerebral amino acid levels and uptake in rats after portocaval anastomosis: I. Regional studies in vitro. Uptake of various amino acids was studied in slices from brain regions of rats four weeks after portocaval anastomosis. No differences of the inulin compartment were observed between control and experimental animals. After 60-minutes incubation, uptake showed an overall pattern of diminution. This was more evident for some amino acids: valine, methionine, and lysine exhibited a lowering of about 30%, which was fairly uniform in the four tested regions; others showed a regional decrease-- alanine in pons-medulla, phenylalanine in cerebellum, histidine and GABA in mesodiencephalon. This decrease did not seen to be related to transport classes. The restricted entry of amino acids into brain cells in portocaval encephalopathy is somewhat difficult to explain; a decreased rate of protein synthesis may be of some importance, but other factors, such as a \"carrier\" impairment, effects on release and on amino acid metabolism, may also be involved."}
{"id": "PMID:469965", "title": "Cerebral amino acid levels and uptake in rats after portocaval anastomosis: II. Regional studies in vivo.", "content": "Amino acid levels have been determined in plasma and in four cerebral regions of rats one month after portocaval shunt. Many plasma amino acids are significantly lowered (asparagine, glutamine, theonine, serine, alanine, valine, leucine, isoleucine, cystine, lysine), while others remain unchanged (taurine, glycine, proline, tryptophan, ornithine, histidine, arginine). Asparagine and glutamine levels are significantly higher than in normal rats, and a net increase of tyrosine (100%), phenylalanine (50%) and citrulline (50%) is evident. In the shunted rat brain the most prominent feature is a very large rise (up to fivefold) of tyrosine, phenylalanine, histidine, citrulline, tryptophan, and glutamine uniformly in the tested regions. Other neutral amino acids are slightly increased. Lysine and arginine are decreased in cerebellum and pons-medulla; taurine, in forebrain and cerebellum. Cerebral permeability to L-amino acids was studied in vivo. Neutral amino acid permeability is greatly increased, whereas basic amino acids show a net decrease in their rate of passage from blood to the brain. No changes are observed for GABA and glutamic acid. These data suggest an altered permeability of the cerebral capillary membranes, which seems to be selective for the different amino acid transport classes. Competitive inhibition experiments demonstrated that the increased brain permeability to neutral amino acids after portocaval shunt is due to an enhancement of the saturable transport. The sharp rise in the brain of some essential neutral amino acids (phenylalanine, tyrosine, trytophan, histidine), largely exceeding their changes in plasma, and the slight cerebral increase of other neutral amino acids despite their lowered level and the rise of competing amino acids in the plasma, is consistent with our observation of enhanced transport for the neutral class. In hepatic encephalopathy, correction of the altered plasma amino acid levels has been reported to improve the clinical status. If this result is connected to the concomitant correction of the brain amino acid levels, carefully selected competitive inhibition among various plasma amino acids could be a useful therapeutic tool in this pathologic condition. However, the increased activity of the neutral amino acid transport system adds a new factor to the problem, since it probably implies that the competing amino acids will accumulate to unphysiological levels in the brain.", "contents": "Cerebral amino acid levels and uptake in rats after portocaval anastomosis: II. Regional studies in vivo. Amino acid levels have been determined in plasma and in four cerebral regions of rats one month after portocaval shunt. Many plasma amino acids are significantly lowered (asparagine, glutamine, theonine, serine, alanine, valine, leucine, isoleucine, cystine, lysine), while others remain unchanged (taurine, glycine, proline, tryptophan, ornithine, histidine, arginine). Asparagine and glutamine levels are significantly higher than in normal rats, and a net increase of tyrosine (100%), phenylalanine (50%) and citrulline (50%) is evident. In the shunted rat brain the most prominent feature is a very large rise (up to fivefold) of tyrosine, phenylalanine, histidine, citrulline, tryptophan, and glutamine uniformly in the tested regions. Other neutral amino acids are slightly increased. Lysine and arginine are decreased in cerebellum and pons-medulla; taurine, in forebrain and cerebellum. Cerebral permeability to L-amino acids was studied in vivo. Neutral amino acid permeability is greatly increased, whereas basic amino acids show a net decrease in their rate of passage from blood to the brain. No changes are observed for GABA and glutamic acid. These data suggest an altered permeability of the cerebral capillary membranes, which seems to be selective for the different amino acid transport classes. Competitive inhibition experiments demonstrated that the increased brain permeability to neutral amino acids after portocaval shunt is due to an enhancement of the saturable transport. The sharp rise in the brain of some essential neutral amino acids (phenylalanine, tyrosine, trytophan, histidine), largely exceeding their changes in plasma, and the slight cerebral increase of other neutral amino acids despite their lowered level and the rise of competing amino acids in the plasma, is consistent with our observation of enhanced transport for the neutral class. In hepatic encephalopathy, correction of the altered plasma amino acid levels has been reported to improve the clinical status. If this result is connected to the concomitant correction of the brain amino acid levels, carefully selected competitive inhibition among various plasma amino acids could be a useful therapeutic tool in this pathologic condition. However, the increased activity of the neutral amino acid transport system adds a new factor to the problem, since it probably implies that the competing amino acids will accumulate to unphysiological levels in the brain."}
{"id": "PMID:469966", "title": "Accidental injury in childhood: a literature review on pediatric trauma.", "content": "This article reviews the literature on accidental injury in childhood, summarizing incidence rates and outlining the factors which have been cited as affecting pediatric trauma: the host, the agents, and the environment. During childhood, age and sex differences for rates of accidental injury are frequently reported. Vehicular accidents, falls, drownings, burns, and ingestions are found to be common agents of injury to pediatric patients. The home affords a virtual breeding ground for accidents to children; however, parents also play an important role in the child's environment, with their illnesses, preoccupations, or dispositions to (in)action having consequences which may eventuate in injury.", "contents": "Accidental injury in childhood: a literature review on pediatric trauma. This article reviews the literature on accidental injury in childhood, summarizing incidence rates and outlining the factors which have been cited as affecting pediatric trauma: the host, the agents, and the environment. During childhood, age and sex differences for rates of accidental injury are frequently reported. Vehicular accidents, falls, drownings, burns, and ingestions are found to be common agents of injury to pediatric patients. The home affords a virtual breeding ground for accidents to children; however, parents also play an important role in the child's environment, with their illnesses, preoccupations, or dispositions to (in)action having consequences which may eventuate in injury."}
{"id": "PMID:469967", "title": "Quality of life after major burns.", "content": "The only presently available method of measuring the outcome of major burns is the mortality rate. We have developed a scale, administered by interview and physical examination, with which the quality of life in survivors may be measured. In an initial group of 32 patients, we took each patient's own preinjury level of performance as a baseline. The size of the burn had no significant effect on the postburn score achieved. Substantial numbers of patients achieved levels superior to their preburn score. Improvement in scores did not begin until 12 months had elapsed from the time of injury. We feel that scales such as this will help determine what happens to patients after their injury, pinpoint areas of weaknesses in the burn program, and enable better comparison of performance between clinical facilities.", "contents": "Quality of life after major burns. The only presently available method of measuring the outcome of major burns is the mortality rate. We have developed a scale, administered by interview and physical examination, with which the quality of life in survivors may be measured. In an initial group of 32 patients, we took each patient's own preinjury level of performance as a baseline. The size of the burn had no significant effect on the postburn score achieved. Substantial numbers of patients achieved levels superior to their preburn score. Improvement in scores did not begin until 12 months had elapsed from the time of injury. We feel that scales such as this will help determine what happens to patients after their injury, pinpoint areas of weaknesses in the burn program, and enable better comparison of performance between clinical facilities."}
{"id": "PMID:469969", "title": "Superior mesenteric artery syndrome in thermally injured patients.", "content": "In a 12-year period, 37 of 3,536 burn patients developed acute obstruction of the duodenum by the superior mesenteric artery. Nasogastric decompression and intravenous fluids were required initially in all 37 patients. Twelve patients received no further therapy, with resolution of the obstruction in six and death of the other six from burn wound sepsis or pneumonia. Ten patients underwent operative treatment consisting of either duodenojejunostomy (eight) or vagotomy and gastroenterostomy (two), with resolution in seven and three postoperative deaths. Fifteen patients were treated with intravenous hyperalimentation and gastric decompression, with resolution in 12 patients, one required operative intervention, and two died of complications. A high degree of suspicion followed by prompt diagnosis and treatment of the SMA syndrome are required in all critically ill patients with unexplained bilious vomiting. Gastric decompression coupled with aggressive use of intravenous hyperalimentation recently has reduced the need for operative treatment (42% versus 11%).", "contents": "Superior mesenteric artery syndrome in thermally injured patients. In a 12-year period, 37 of 3,536 burn patients developed acute obstruction of the duodenum by the superior mesenteric artery. Nasogastric decompression and intravenous fluids were required initially in all 37 patients. Twelve patients received no further therapy, with resolution of the obstruction in six and death of the other six from burn wound sepsis or pneumonia. Ten patients underwent operative treatment consisting of either duodenojejunostomy (eight) or vagotomy and gastroenterostomy (two), with resolution in seven and three postoperative deaths. Fifteen patients were treated with intravenous hyperalimentation and gastric decompression, with resolution in 12 patients, one required operative intervention, and two died of complications. A high degree of suspicion followed by prompt diagnosis and treatment of the SMA syndrome are required in all critically ill patients with unexplained bilious vomiting. Gastric decompression coupled with aggressive use of intravenous hyperalimentation recently has reduced the need for operative treatment (42% versus 11%)."}
{"id": "PMID:469970", "title": "Posterior fracture-dislocation of the hip: an epidemiologic review.", "content": "A retrospective study of 46 posterior fracture-dislocations of the hip showed that reduction of the dislocation within 24 hours was the most important initial treatment. Open reduction and fixation of the fracture could be delayed up to 4 weeks to allow associated injuries to be treated, and this technique was indicated to remove intra-articular bone fragments or for residual instability. Long-term disability resulted in every patient, regardless of the severity of the initial injury or the method of treatment. Half of the patients never returned to work. Of the 46 patients 43 were unrestrained occupants in vehicular accidents. Multiple injury occurred in 75% of the patients.", "contents": "Posterior fracture-dislocation of the hip: an epidemiologic review. A retrospective study of 46 posterior fracture-dislocations of the hip showed that reduction of the dislocation within 24 hours was the most important initial treatment. Open reduction and fixation of the fracture could be delayed up to 4 weeks to allow associated injuries to be treated, and this technique was indicated to remove intra-articular bone fragments or for residual instability. Long-term disability resulted in every patient, regardless of the severity of the initial injury or the method of treatment. Half of the patients never returned to work. Of the 46 patients 43 were unrestrained occupants in vehicular accidents. Multiple injury occurred in 75% of the patients."}
{"id": "PMID:469971", "title": "Subtrochanteric fractures of the femur: a review of 130 patients.", "content": "A review of 130 patients with subtrochanteric fractures of the femur has led to a new classification based upon fracture pattern. Type I is a short oblique, Type II a long oblique, and Type III any comminuted fracture of the subtrochanteric area. Twenty-two patients were treated with traction and 108 patients treated by surgical operation. Stable fractures treated in skin traction did well and extensively comminuted fractures appeared to be best treated with 90-90 skeletal traction. In the Type I and Type II fractures, it was determined that these fracture patterns were stable and any strong nail-plate device will provide adequate fixation. The Type III fracture may be converted to Type I or Type II by lag screw fixation and then fixed accordingly but if stability cannot be obtained by this method, a sliding screw type of device appeared to offer the best fixation. Supplementary bone graft where necessary to restore the medial buttress offered singularly better healing. Delayed weight bearing until fracture healing was secure gave the best chance of primary bone healing without deformity.", "contents": "Subtrochanteric fractures of the femur: a review of 130 patients. A review of 130 patients with subtrochanteric fractures of the femur has led to a new classification based upon fracture pattern. Type I is a short oblique, Type II a long oblique, and Type III any comminuted fracture of the subtrochanteric area. Twenty-two patients were treated with traction and 108 patients treated by surgical operation. Stable fractures treated in skin traction did well and extensively comminuted fractures appeared to be best treated with 90-90 skeletal traction. In the Type I and Type II fractures, it was determined that these fracture patterns were stable and any strong nail-plate device will provide adequate fixation. The Type III fracture may be converted to Type I or Type II by lag screw fixation and then fixed accordingly but if stability cannot be obtained by this method, a sliding screw type of device appeared to offer the best fixation. Supplementary bone graft where necessary to restore the medial buttress offered singularly better healing. Delayed weight bearing until fracture healing was secure gave the best chance of primary bone healing without deformity."}
{"id": "PMID:469972", "title": "Fractures of the distal tibial metaphysis with intra-articular extension--the distal tibial explosion fracture.", "content": "In a study of 26 distal tibial explosion fractures in 24 patients two fracture patterns were identified: Type A, a rotational pattern; and Type B, a compressive fracture pattern. The rotational pattern was found to be less common than the compressive fracture and to have a substantially better prognosis. Operative treatment of the Type A pattern led to an overall acceptable result of 84%, while the Type B patients had 53% acceptable results. The results of operative treatment were however, superior to the results of nonoperative treatment in both fracture groups. Adequate treatment consists of anatomic reconstruction of the distal tibia, rigid internal fixation, early active motion, and nonweight bearing in 3 to 5 months.", "contents": "Fractures of the distal tibial metaphysis with intra-articular extension--the distal tibial explosion fracture. In a study of 26 distal tibial explosion fractures in 24 patients two fracture patterns were identified: Type A, a rotational pattern; and Type B, a compressive fracture pattern. The rotational pattern was found to be less common than the compressive fracture and to have a substantially better prognosis. Operative treatment of the Type A pattern led to an overall acceptable result of 84%, while the Type B patients had 53% acceptable results. The results of operative treatment were however, superior to the results of nonoperative treatment in both fracture groups. Adequate treatment consists of anatomic reconstruction of the distal tibia, rigid internal fixation, early active motion, and nonweight bearing in 3 to 5 months."}
{"id": "PMID:469973", "title": "Management of portal vein injuries.", "content": "Injuries to the portal vein are associated with a high mortality because of a high incidence of concomitant injury to surrounding structures and refractory shock. Repair of the portal vein injury is often difficult or impossible because of massive hemorrhage. The key to successful management of a portal vein injury is rapid blood volume resuscitation and obtaining rapid and adequate exposure. The optimal exposure for repair consists of reflection of the hepatic flexure of the colon with mobilization of the root of the mesentery, pancreas, and duodenum. Lateral venorrhaphy is the preferred method of management, but in hemodynamically unstable patients, ligation of the portal vein is an acceptable method of treatment.", "contents": "Management of portal vein injuries. Injuries to the portal vein are associated with a high mortality because of a high incidence of concomitant injury to surrounding structures and refractory shock. Repair of the portal vein injury is often difficult or impossible because of massive hemorrhage. The key to successful management of a portal vein injury is rapid blood volume resuscitation and obtaining rapid and adequate exposure. The optimal exposure for repair consists of reflection of the hepatic flexure of the colon with mobilization of the root of the mesentery, pancreas, and duodenum. Lateral venorrhaphy is the preferred method of management, but in hemodynamically unstable patients, ligation of the portal vein is an acceptable method of treatment."}
{"id": "PMID:469974", "title": "Correlations between metabolic and cardiopulmonary measurements in patients after trauma, general surgery, and sepsis.", "content": "Simultaneous longitudinal hormonometabolic-physiologic (cardiopulmonary) profiles were measured in 14 nonseptic trauma/general surgery (T/GS) patients and in ten patients with Gram-negative abdominal surgical sepsis. The physiologic state classification system was used as the frame of reference. There were no response differences between the T and GS groups: they had A State responses. The sepsis (S) patients initially had exaggerated A State responses with significant changes in glucose, fat, amino acid, and glucagon plasma levels relative to T/GS. The S patients who survived (four) demonstrated profiles as in T/GS. The S patients who expired (six) progressively evolved an unbalanced, hyperdynamic B State response with progressive elevations of glucose, lactate, aromatic and branched-chain amino acids and glucagon, and low ketone bodies. There is definite correlation over time between metabolic and physiologic responses; the physiologic responses reflect the metabolic responses; the metabolic responses are consistent with a peripheral energy-fuel deficit.", "contents": "Correlations between metabolic and cardiopulmonary measurements in patients after trauma, general surgery, and sepsis. Simultaneous longitudinal hormonometabolic-physiologic (cardiopulmonary) profiles were measured in 14 nonseptic trauma/general surgery (T/GS) patients and in ten patients with Gram-negative abdominal surgical sepsis. The physiologic state classification system was used as the frame of reference. There were no response differences between the T and GS groups: they had A State responses. The sepsis (S) patients initially had exaggerated A State responses with significant changes in glucose, fat, amino acid, and glucagon plasma levels relative to T/GS. The S patients who survived (four) demonstrated profiles as in T/GS. The S patients who expired (six) progressively evolved an unbalanced, hyperdynamic B State response with progressive elevations of glucose, lactate, aromatic and branched-chain amino acids and glucagon, and low ketone bodies. There is definite correlation over time between metabolic and physiologic responses; the physiologic responses reflect the metabolic responses; the metabolic responses are consistent with a peripheral energy-fuel deficit."}
{"id": "PMID:469975", "title": "Cervical osteomyelitis complicating transpharyngeal gunshot wounds to the neck.", "content": "Four cases of cervical osteomyelitis complicating transpharyngeal gunshot wounds to the neck are discussed. Thorough debridement of bone and soft tissue, adequate drainage, broad-spectrum antibiotics, and rigid immobilization of the cervical spine are essential for the successful treatment of this complication. Inadequate or delayed treatment may result in prolonged hospitalization, the need for multiple surgical procedures, and possible neurologic deficits.", "contents": "Cervical osteomyelitis complicating transpharyngeal gunshot wounds to the neck. Four cases of cervical osteomyelitis complicating transpharyngeal gunshot wounds to the neck are discussed. Thorough debridement of bone and soft tissue, adequate drainage, broad-spectrum antibiotics, and rigid immobilization of the cervical spine are essential for the successful treatment of this complication. Inadequate or delayed treatment may result in prolonged hospitalization, the need for multiple surgical procedures, and possible neurologic deficits."}
{"id": "PMID:469976", "title": "Complete disruption of axillary artery caused by severe atherosclerosis and trivial nonpenetrating trauma.", "content": "Complete disruption of the axillary artery related to relatively trivial nonpenetrating trauma in a 67-year-old male is reported. The injury occurred in relation to severe circumferential atherosclerosis of the vessel. Successful revascularization was accomplished with salvage of the extremity and normal function.", "contents": "Complete disruption of axillary artery caused by severe atherosclerosis and trivial nonpenetrating trauma. Complete disruption of the axillary artery related to relatively trivial nonpenetrating trauma in a 67-year-old male is reported. The injury occurred in relation to severe circumferential atherosclerosis of the vessel. Successful revascularization was accomplished with salvage of the extremity and normal function."}
{"id": "PMID:469979", "title": "Salmonella gastroenteritis in Hong Kong--a clinical review of 200 patients.", "content": "A sudden increase in Salmonella gastroenteritis affecting infants and children in Hong Kong in 1971 prompted a clinical review of 200 such patients seen over a 6 year period. It showed unprecedented prevalence of Salmonella johannesburg infections and unusually protracted diarrhoea. Only 3.5 per cent of patients were breast fed. Factors causing this chronicity are discussed. All 8 fatal cases were under 7 1/2 months old, with protracted diarrhoea starting within 1 month after birth in 7. The invasiveness of Salmonella johannesburg is low although its infectivity high. Bacteraemia occurred in only 1 patient and focal infections other than gastroenteritis in none. Antibiotics did not improve diarrhoea nor eliminate faecal excretion in the majority of those treated. Prolonged and intermittent faecal excretion of Salmonella was common. Nineteen per cent of patients acquired diarrhoea in hospital; some after a course of antibiotics given for other infections. Experience from this series does not recommend administration of antibiotics to patients with uncomplicated Salmonella johannesburg gastroenteritis. As chronicity of diarrhoea seemed to be the major prognostic factor with regard to mortality and morbidity in this series, further search for causes and control measures of this chronicity is required.", "contents": "Salmonella gastroenteritis in Hong Kong--a clinical review of 200 patients. A sudden increase in Salmonella gastroenteritis affecting infants and children in Hong Kong in 1971 prompted a clinical review of 200 such patients seen over a 6 year period. It showed unprecedented prevalence of Salmonella johannesburg infections and unusually protracted diarrhoea. Only 3.5 per cent of patients were breast fed. Factors causing this chronicity are discussed. All 8 fatal cases were under 7 1/2 months old, with protracted diarrhoea starting within 1 month after birth in 7. The invasiveness of Salmonella johannesburg is low although its infectivity high. Bacteraemia occurred in only 1 patient and focal infections other than gastroenteritis in none. Antibiotics did not improve diarrhoea nor eliminate faecal excretion in the majority of those treated. Prolonged and intermittent faecal excretion of Salmonella was common. Nineteen per cent of patients acquired diarrhoea in hospital; some after a course of antibiotics given for other infections. Experience from this series does not recommend administration of antibiotics to patients with uncomplicated Salmonella johannesburg gastroenteritis. As chronicity of diarrhoea seemed to be the major prognostic factor with regard to mortality and morbidity in this series, further search for causes and control measures of this chronicity is required."}
{"id": "PMID:469980", "title": "A school survey of tinea capitis in Benghazi, Libya.", "content": "A school survey of Tinea capitis in Benghazi revealed an incidence of 4.49 per cent. The commonest clinical type which accounted for 87.7 per cent of the cases, resembled Seborrhoea capitis more than with any classical type described so far. T. schoenleinii and T. violaeceum were the most frequent isolates.", "contents": "A school survey of tinea capitis in Benghazi, Libya. A school survey of Tinea capitis in Benghazi revealed an incidence of 4.49 per cent. The commonest clinical type which accounted for 87.7 per cent of the cases, resembled Seborrhoea capitis more than with any classical type described so far. T. schoenleinii and T. violaeceum were the most frequent isolates."}
{"id": "PMID:469982", "title": "Evaluation of the tuberculin sensitivity test in a healthy Sudanese population.", "content": "Three-hundred and seventeen healthy individuals were investigated on a volunteer basis for tuberculin sensitivity using the dual-intradermal tuberculin test technique. 251 of them were in contact with dairy animals and 64.9 per cent of such people were found specific reactors. Those who were not in contact with dairy animals (66 persons) gave 65.2 per cent rate of specific reactivity. To interpret the tuberculin sensitivity reaction, non-specific reactivity must be excluded, especially in tropical countries. Positive tuberculin reaction is defined when the diameter of induration is 10 mm or more for persons in contact with dairy animals and tuberculous patients or consuming raw milk and 5 mm or more for other persons.", "contents": "Evaluation of the tuberculin sensitivity test in a healthy Sudanese population. Three-hundred and seventeen healthy individuals were investigated on a volunteer basis for tuberculin sensitivity using the dual-intradermal tuberculin test technique. 251 of them were in contact with dairy animals and 64.9 per cent of such people were found specific reactors. Those who were not in contact with dairy animals (66 persons) gave 65.2 per cent rate of specific reactivity. To interpret the tuberculin sensitivity reaction, non-specific reactivity must be excluded, especially in tropical countries. Positive tuberculin reaction is defined when the diameter of induration is 10 mm or more for persons in contact with dairy animals and tuberculous patients or consuming raw milk and 5 mm or more for other persons."}
{"id": "PMID:469983", "title": "Primary amoebic meningoencephalitis in Nigeria (report of two cases in children).", "content": "Two cases of PAME in children occurring during dusty harmattan period in Northern Nigeria are reported. In the absence of history of swimming or any other water related sport, and as suggested in our two previous reports, a dustbone infection as an important route of infection in PAME in this area is stressed. A need to fully investigate any atypical case of meningitis and meningoencephalitis in this area is emphasised.", "contents": "Primary amoebic meningoencephalitis in Nigeria (report of two cases in children). Two cases of PAME in children occurring during dusty harmattan period in Northern Nigeria are reported. In the absence of history of swimming or any other water related sport, and as suggested in our two previous reports, a dustbone infection as an important route of infection in PAME in this area is stressed. A need to fully investigate any atypical case of meningitis and meningoencephalitis in this area is emphasised."}
{"id": "PMID:469990", "title": "Uncoating of adenovirus type 2.", "content": "The uncoating of adenovirus type 2 and a temperature-sensitive mutant, tsl, was studied. HEp-2 cells were infected with 32P- OR 125I-labeled purified virions for various lengths of time, and the nuclear and cytoplasmic fractions were analyzed by sucrose gradient velocity sedimentation and sodium dodecyl sulfate-polyacryl-amide gel electrophoresis. Within 1 h of infection, virions were converted into three subviral structures: (1) subviral structures in the cytoplasm with a density greater than virions but which qualitatively still contained all virus polypeptides; (ii) corelike structures associated with both the nuclear and cytoplasmic fractions and composed of viral DNA and polypeptides VIa2, V and PVII; and (iii) putative DNA-terminal protein complexes in the nuclei. The kinetic and compartmentalization studies suggested that the DNA-terminal protein complex is the end product of uncoating. The virions which were synthesized by tsl at the nonpermissive temperature and contained the precursor polypeptides PVI and PVII were found to be blocked in uncoating at the corelike stage. This block in uncoating provides the explanation for the lack of infectivity of these virions. A model for the uncoating of adenovirus is proposed.", "contents": "Uncoating of adenovirus type 2. The uncoating of adenovirus type 2 and a temperature-sensitive mutant, tsl, was studied. HEp-2 cells were infected with 32P- OR 125I-labeled purified virions for various lengths of time, and the nuclear and cytoplasmic fractions were analyzed by sucrose gradient velocity sedimentation and sodium dodecyl sulfate-polyacryl-amide gel electrophoresis. Within 1 h of infection, virions were converted into three subviral structures: (1) subviral structures in the cytoplasm with a density greater than virions but which qualitatively still contained all virus polypeptides; (ii) corelike structures associated with both the nuclear and cytoplasmic fractions and composed of viral DNA and polypeptides VIa2, V and PVII; and (iii) putative DNA-terminal protein complexes in the nuclei. The kinetic and compartmentalization studies suggested that the DNA-terminal protein complex is the end product of uncoating. The virions which were synthesized by tsl at the nonpermissive temperature and contained the precursor polypeptides PVI and PVII were found to be blocked in uncoating at the corelike stage. This block in uncoating provides the explanation for the lack of infectivity of these virions. A model for the uncoating of adenovirus is proposed."}
{"id": "PMID:469991", "title": "Cell wall lysin as a component of the bacteriophage phi 6 virion.", "content": "Cell wall lytic activity was found in particles of the lipid-containing bacteriophage \u00f86. The activity can be extracted from the virion with Triton X-100 in the presence of salt. This treatment removes the membrane-like envelope of the virion which includes five proteins. The lysin requires detergent for in vitro activity. Virus particles formed in nonsuppressor cells by several classes of \u00f86 nonsense mutants contained the lysin activity; however, particles formed by a mutant (unable to make proteins P5 and P11) had very low activity; high activity was produced when particles were formed in a suppressor host. A study of the time course of the appearance of the lysin during infection showed that it appeared and increased in cells infected with wild-type virus and in suppressor cells infected with a mutant of class 511, but it did not increase in nonsuppressor cells infected with the class 511 mutant. It is concluded that protein P5 is a component of the lysin and that the role of its activity is in both early and late stages of infection. In particular, the lysin may be necessary for the passage of the infecting core of the virion through the cell wall of the bacterium, as well as in the final lysis necessary for the liberation of progeny phage. A mutant of the virus that produces a larger-than-normal protein P10 does not induce normal lysin activity in host Pseudomonas phaseolicola HB10Y, although it does in strain ERA Pseudomonas pseudoalcaligenes. This indicates that protein P5 is probably not sufficient for lysin activity, but the nature of the interaction between P5 and P10 is unknown.", "contents": "Cell wall lysin as a component of the bacteriophage phi 6 virion. Cell wall lytic activity was found in particles of the lipid-containing bacteriophage \u00f86. The activity can be extracted from the virion with Triton X-100 in the presence of salt. This treatment removes the membrane-like envelope of the virion which includes five proteins. The lysin requires detergent for in vitro activity. Virus particles formed in nonsuppressor cells by several classes of \u00f86 nonsense mutants contained the lysin activity; however, particles formed by a mutant (unable to make proteins P5 and P11) had very low activity; high activity was produced when particles were formed in a suppressor host. A study of the time course of the appearance of the lysin during infection showed that it appeared and increased in cells infected with wild-type virus and in suppressor cells infected with a mutant of class 511, but it did not increase in nonsuppressor cells infected with the class 511 mutant. It is concluded that protein P5 is a component of the lysin and that the role of its activity is in both early and late stages of infection. In particular, the lysin may be necessary for the passage of the infecting core of the virion through the cell wall of the bacterium, as well as in the final lysis necessary for the liberation of progeny phage. A mutant of the virus that produces a larger-than-normal protein P10 does not induce normal lysin activity in host Pseudomonas phaseolicola HB10Y, although it does in strain ERA Pseudomonas pseudoalcaligenes. This indicates that protein P5 is probably not sufficient for lysin activity, but the nature of the interaction between P5 and P10 is unknown."}
{"id": "PMID:469992", "title": "Differential translation in normal and adenovirus type 5-infected human cells and cell-free systems.", "content": "When uninfected or adenovirus 5-infected KB cells are exposed to hypertonic medium, the incorporation of radioactive amino acids into protein decreases in both, but more severely in the uninfected cells. Although the effect of hypertonic medium on the synthesis of specific polypeptides varies, the translation of viral polypeptides as a class is less inhibited. The same patterns of proteins are synthesized regardless of the solute used in the hypertonic medium. The mechanism by which hypertonic conditions exert their effect on whole cells was investigated in K cell-free systems. It was possible to simulate the differential patterns of protein synthesis obtained in whole cells in hypertonic medium by increasing ion concentrations in cell-free extracts which are capable of initiating polypeptide chains on exogenous templates. However, in cell lysates which only elongate proteins, the same patterns were not obtained. Certain host and viral polypeptides displayed striking responses to increased ionic conditions in whole cells and cell-free systems. The synthesis of a host 44K protein, actin, appeared to be most sensitive; lower-molecular-weight proteins were fairly resistant. Among the viral proteins, the synthesis of 100K was inhibited, but most notable was the marked resistance of the synthesis of polypeptide IX. Possible mechanisms for differential synthesis and their significance are considered.", "contents": "Differential translation in normal and adenovirus type 5-infected human cells and cell-free systems. When uninfected or adenovirus 5-infected KB cells are exposed to hypertonic medium, the incorporation of radioactive amino acids into protein decreases in both, but more severely in the uninfected cells. Although the effect of hypertonic medium on the synthesis of specific polypeptides varies, the translation of viral polypeptides as a class is less inhibited. The same patterns of proteins are synthesized regardless of the solute used in the hypertonic medium. The mechanism by which hypertonic conditions exert their effect on whole cells was investigated in K cell-free systems. It was possible to simulate the differential patterns of protein synthesis obtained in whole cells in hypertonic medium by increasing ion concentrations in cell-free extracts which are capable of initiating polypeptide chains on exogenous templates. However, in cell lysates which only elongate proteins, the same patterns were not obtained. Certain host and viral polypeptides displayed striking responses to increased ionic conditions in whole cells and cell-free systems. The synthesis of a host 44K protein, actin, appeared to be most sensitive; lower-molecular-weight proteins were fairly resistant. Among the viral proteins, the synthesis of 100K was inhibited, but most notable was the marked resistance of the synthesis of polypeptide IX. Possible mechanisms for differential synthesis and their significance are considered."}
{"id": "PMID:469993", "title": "gag-Related polyproteins of Moloney murine leukemia virus: evidence for independent synthesis of glycosylated and unglycosylated forms.", "content": "Immunoprecipitation of labeled extracts from murine leukemia virus-infected cells with antisera specific for internal structural (gag) proteins yields three major gag-related polyproteins with molecular weights of 180,000 (Pr180gag-pol), 80,000, and 65,000 (Pr65gag). It has been shown by others that Pr65gag is the immediate precursor of the internal structural (gag) protein, and that Pr180gag-pol is the precursor to reverse transcriptase. In studies reported here, the 80,000-dalton gag-related polyprotein from Moloney strain murine leukemia virus (M-MuLV)-infected cells was found to be glycosylated by the following criteria: (i) incorporation of [3H]mannose, (ii) a change in electrophoretic mobility upon digestion with endoglycosidase H, and (iii) a change in electrophoretic mobility when glycosylation was inhibited by treatment of the cells with tunicamycin during labeling. The 80,000-dalton gag polyprotein has therefore been designated GpP80gag. The unglycosylated form of GpP80gag was a polypeptide of 75,000 daltons. A comparison of [3H]mannose and [3H]galactose labeling experiments suggested that GpP80gag is further glycosylated to yield a glycopolypeptide of 95,000 daltons. This 95,000-dalton polypeptide is relatively rapidly cleaved to yield two glycopeptides of 55,000 and 40,000 daltons which are released into the cell culture fluid, as soluble proteins. Cell-free translation of M-MuLV genomic RNA resulted in two major gag-related products of 75,000 and 65,000 daltons. The 65,000-dalton gag-related cell-free translation product comigrated with Pr65gag, and the 75,000-dalton cell-free product comigrated with the unglycosylated form of GpP80gag. Both of the gag-related cell-free translation products could be labeled with [35S]formyl methionine, which is incorporated only as the N-terminal amino acid during translation. Other investigators have shown that GpP80gag and Pr65gag differ at their N-termini, and these results combined with those reported here suggest that GpP80gag and Pr65gag are translated from two separate initiation sites in M-MuLV RNA.", "contents": "gag-Related polyproteins of Moloney murine leukemia virus: evidence for independent synthesis of glycosylated and unglycosylated forms. Immunoprecipitation of labeled extracts from murine leukemia virus-infected cells with antisera specific for internal structural (gag) proteins yields three major gag-related polyproteins with molecular weights of 180,000 (Pr180gag-pol), 80,000, and 65,000 (Pr65gag). It has been shown by others that Pr65gag is the immediate precursor of the internal structural (gag) protein, and that Pr180gag-pol is the precursor to reverse transcriptase. In studies reported here, the 80,000-dalton gag-related polyprotein from Moloney strain murine leukemia virus (M-MuLV)-infected cells was found to be glycosylated by the following criteria: (i) incorporation of [3H]mannose, (ii) a change in electrophoretic mobility upon digestion with endoglycosidase H, and (iii) a change in electrophoretic mobility when glycosylation was inhibited by treatment of the cells with tunicamycin during labeling. The 80,000-dalton gag polyprotein has therefore been designated GpP80gag. The unglycosylated form of GpP80gag was a polypeptide of 75,000 daltons. A comparison of [3H]mannose and [3H]galactose labeling experiments suggested that GpP80gag is further glycosylated to yield a glycopolypeptide of 95,000 daltons. This 95,000-dalton polypeptide is relatively rapidly cleaved to yield two glycopeptides of 55,000 and 40,000 daltons which are released into the cell culture fluid, as soluble proteins. Cell-free translation of M-MuLV genomic RNA resulted in two major gag-related products of 75,000 and 65,000 daltons. The 65,000-dalton gag-related cell-free translation product comigrated with Pr65gag, and the 75,000-dalton cell-free product comigrated with the unglycosylated form of GpP80gag. Both of the gag-related cell-free translation products could be labeled with [35S]formyl methionine, which is incorporated only as the N-terminal amino acid during translation. Other investigators have shown that GpP80gag and Pr65gag differ at their N-termini, and these results combined with those reported here suggest that GpP80gag and Pr65gag are translated from two separate initiation sites in M-MuLV RNA."}
{"id": "PMID:469994", "title": "Glycoprotein encoded by the Friend spleen focus-forming virus.", "content": "The Friend spleen focus-forming virus (F-SFFV) released from cultured erythroleukemia cells (cell line F4-6/K) was cloned free of its helper lymphatic leukemia virus (F-MuLV). After allowing adsorption to Sc-1 fibroblasts at a low multiplicity of infection, the cells were seeded individually into wells of a microtitier test plate and the resulting colonies were grown into large cultures. Among 14 of these cell cultures that have been analyzed thoroughly, 6 contained F-SFFV alone, 1 contained F-MuLV plus F-SFFV, and 7 were uninfected. Each of the Sc-1 cell lines which had been infected with cloned F-SFFV contained a glycoprotein with an apparent molecular weight of 55,000 (gp55) that was absent from the cell lines that lacked F-SFFV. gp55 was also present in Friend erythroleukemia cells and in fibroblasts infected with an F-SFFV that had been doubly cloned in another laboratory. These results indicate that gp55 is encoded by the F-SFFV genome. gp55 has the following additional properties. It can be immunoprecipitated with antiserum made to the F-MuLV virion envelope glycoprotein (gp75). Its unglycosylated polypeptide, formed in cells treated with 2-deoxy-D-glucose, has a molecular weight of approximately 45,000. Its tryptic peptide map contains peptides in common with F-MuLV gp75 but it also contains unique peptides. It appears to be absent or present in only low concentrations in erythroleukemia cell plasma membranes as determined by lactoperoxidase-catalyzed iodination, and it accumulates intracellularly in large amounts. In addition, it is absent from released virions. The majority of the cellular gp55 has an isoelectric point of 8.5 to 9.0. These results are consistent with the idea that an env gene recombination event was involved in the origin of F-SFFV.", "contents": "Glycoprotein encoded by the Friend spleen focus-forming virus. The Friend spleen focus-forming virus (F-SFFV) released from cultured erythroleukemia cells (cell line F4-6/K) was cloned free of its helper lymphatic leukemia virus (F-MuLV). After allowing adsorption to Sc-1 fibroblasts at a low multiplicity of infection, the cells were seeded individually into wells of a microtitier test plate and the resulting colonies were grown into large cultures. Among 14 of these cell cultures that have been analyzed thoroughly, 6 contained F-SFFV alone, 1 contained F-MuLV plus F-SFFV, and 7 were uninfected. Each of the Sc-1 cell lines which had been infected with cloned F-SFFV contained a glycoprotein with an apparent molecular weight of 55,000 (gp55) that was absent from the cell lines that lacked F-SFFV. gp55 was also present in Friend erythroleukemia cells and in fibroblasts infected with an F-SFFV that had been doubly cloned in another laboratory. These results indicate that gp55 is encoded by the F-SFFV genome. gp55 has the following additional properties. It can be immunoprecipitated with antiserum made to the F-MuLV virion envelope glycoprotein (gp75). Its unglycosylated polypeptide, formed in cells treated with 2-deoxy-D-glucose, has a molecular weight of approximately 45,000. Its tryptic peptide map contains peptides in common with F-MuLV gp75 but it also contains unique peptides. It appears to be absent or present in only low concentrations in erythroleukemia cell plasma membranes as determined by lactoperoxidase-catalyzed iodination, and it accumulates intracellularly in large amounts. In addition, it is absent from released virions. The majority of the cellular gp55 has an isoelectric point of 8.5 to 9.0. These results are consistent with the idea that an env gene recombination event was involved in the origin of F-SFFV."}
{"id": "PMID:469995", "title": "Tryptic peptide analysis of gag and gag-pol gene products of Rauscher murine leukemia virus.", "content": "[3H]tyrosine-labeled viral precursor polyproteins and known mature viral proteins derived from the Rauscher murine leukemia virus gag and pol genes were examined by two-dimensional tryptic peptide mapping. Pr200gag-pol was found to contain peptide sequences of the viral core proteins p30, p15, p12, and p10, as well as peptide sequences found in the cell-associated reverse transcriptase. Intermediate reverse transcriptase precursor Pr125pol lacked peptide sequences of the four-core proteins but contained reverse transcriptase-specific tryptic peptides plus two additional tyrosine-containing tryptic peptides not related to gag or pol gene products. Methionine-containing tryptic peptide analysis also suggested the presence of additional protein material in Pr125pol (Kopchick et al., Proc. Natl. Acad. Sci. U.S.A. 75:2016-2020, 1978). Pr200gag-pol, although containing both viral core and reverse transcriptase-assoicated methionine and tyrosine tryptic peptides, also contained additional tryptic peptides. Thes are of two classes: (i) tryptic peptides associated with the Pr125pol but not Pr80pol and (ii) tryptic peptides not found in Pr125pol or in any known viral protein. One interpretation of these results is that Pr200gag-pol contains additional gene products aside from the gag and pol genes. Pr80gag and Pr65gag peptide maps were also examined and found to have sequences of all four core proteins. Pr65gag was found to contain two p30 tyrosine tryptic peptides that were absent in Pr80gag, suggesting that Pr80gag may not be the precursor to Pr65gag. Pr80gag, as expected from its larger size, also contained tryptic peptides not found in Pr65gag. Two of these additional Pr80gag tryptic peptides were found in Pr80pol as well but not in any of the viral core proteins, suggesting that Pr80gag and Pr80pol may have overlapping peptide sequences. Consistent with this finding is the conclusion that Pr80gag terminates within the pol gene. A model that describes the relationship of these recent findings to viral gene products is presented.", "contents": "Tryptic peptide analysis of gag and gag-pol gene products of Rauscher murine leukemia virus. [3H]tyrosine-labeled viral precursor polyproteins and known mature viral proteins derived from the Rauscher murine leukemia virus gag and pol genes were examined by two-dimensional tryptic peptide mapping. Pr200gag-pol was found to contain peptide sequences of the viral core proteins p30, p15, p12, and p10, as well as peptide sequences found in the cell-associated reverse transcriptase. Intermediate reverse transcriptase precursor Pr125pol lacked peptide sequences of the four-core proteins but contained reverse transcriptase-specific tryptic peptides plus two additional tyrosine-containing tryptic peptides not related to gag or pol gene products. Methionine-containing tryptic peptide analysis also suggested the presence of additional protein material in Pr125pol (Kopchick et al., Proc. Natl. Acad. Sci. U.S.A. 75:2016-2020, 1978). Pr200gag-pol, although containing both viral core and reverse transcriptase-assoicated methionine and tyrosine tryptic peptides, also contained additional tryptic peptides. Thes are of two classes: (i) tryptic peptides associated with the Pr125pol but not Pr80pol and (ii) tryptic peptides not found in Pr125pol or in any known viral protein. One interpretation of these results is that Pr200gag-pol contains additional gene products aside from the gag and pol genes. Pr80gag and Pr65gag peptide maps were also examined and found to have sequences of all four core proteins. Pr65gag was found to contain two p30 tyrosine tryptic peptides that were absent in Pr80gag, suggesting that Pr80gag may not be the precursor to Pr65gag. Pr80gag, as expected from its larger size, also contained tryptic peptides not found in Pr65gag. Two of these additional Pr80gag tryptic peptides were found in Pr80pol as well but not in any of the viral core proteins, suggesting that Pr80gag and Pr80pol may have overlapping peptide sequences. Consistent with this finding is the conclusion that Pr80gag terminates within the pol gene. A model that describes the relationship of these recent findings to viral gene products is presented."}
{"id": "PMID:469996", "title": "Normal saline slush preparation for renal surgery.", "content": "We describe a simple, rapid method to prepare normal saline slush with commercially available slush mugs that are gas-sterilized in routine fashion and stored in freezer. Slush can be prepared intraoperatively in 10 minutes with this method.", "contents": "Normal saline slush preparation for renal surgery. We describe a simple, rapid method to prepare normal saline slush with commercially available slush mugs that are gas-sterilized in routine fashion and stored in freezer. Slush can be prepared intraoperatively in 10 minutes with this method."}
{"id": "PMID:469997", "title": "Prevalence rate of renal stone disease in Forsyth County, North Carolina during 1977.", "content": "A count of all recorded hospitalized cases of Forsyth County, North Carolina residents with a primary discharge diagnosis of renal (kidneyand/or ureteral) stone in 1977 is obtained by age, race and sex, and used in conjunction with the census data to estimate age-adjusted prevalence rates. The over-all prevalence rate is 2.08 per thousand population, which agrees well with results of earlier investigations. For white male subjects the age-adjusted prevalence rate is 3.64 per thousand, for white female subjects 1.44, for non-white male subjects 0.97 and for non-white female subjects 0.34. The white to non-white ratio and the male to female rates are compared with earlier published values. For all groups the prevalence rate increases with age, attaining a maximum in the 40 to 60-year category for whites and somewhat later for non-whites. Beyond age 60 prevalence drops, reaching 0 or near 0 in the 80 to 89-year range. A simple phenomenological model is suggested to explain the observed race and sex differences in the prevalence rates.", "contents": "Prevalence rate of renal stone disease in Forsyth County, North Carolina during 1977. A count of all recorded hospitalized cases of Forsyth County, North Carolina residents with a primary discharge diagnosis of renal (kidneyand/or ureteral) stone in 1977 is obtained by age, race and sex, and used in conjunction with the census data to estimate age-adjusted prevalence rates. The over-all prevalence rate is 2.08 per thousand population, which agrees well with results of earlier investigations. For white male subjects the age-adjusted prevalence rate is 3.64 per thousand, for white female subjects 1.44, for non-white male subjects 0.97 and for non-white female subjects 0.34. The white to non-white ratio and the male to female rates are compared with earlier published values. For all groups the prevalence rate increases with age, attaining a maximum in the 40 to 60-year category for whites and somewhat later for non-whites. Beyond age 60 prevalence drops, reaching 0 or near 0 in the 80 to 89-year range. A simple phenomenological model is suggested to explain the observed race and sex differences in the prevalence rates."}
{"id": "PMID:469998", "title": "The detection and clinical significance of antibodies to tumor-associated antigens in patients with renal cell carcinoma.", "content": "Antibodies against renal carcinoma cells were detected by a microcomplement fixation assay in the sera of 94 per cent of 75 patients with recently resected, localized or metastatic disease, and in 20 per cent of normal controls. Absorption of sera with pooled normal kidney cells abrogated reactivity against normal kidney cells but did not decrease reactivity significantly against the renal carcinoma cells. Analyses of sequential serum samples revealed that after nephrectomy in 9 of 10 patients free of tumor but a high risk for recurrence antibody levels decreased or disappeared by 1 year. However, these levels remained elevated in 2 patients who subsequently were found to have occult persistent tumor. Antibody titers in patients with metastatic renal cell carcinoma declined as the disease progressed. Patients with persistently elevated titers had the longest survival. Although the antibodies detected by this assay may not be specific for renal cell carcinoma, their detection may be of clinical significance with regard to the prognosis of the disease.", "contents": "The detection and clinical significance of antibodies to tumor-associated antigens in patients with renal cell carcinoma. Antibodies against renal carcinoma cells were detected by a microcomplement fixation assay in the sera of 94 per cent of 75 patients with recently resected, localized or metastatic disease, and in 20 per cent of normal controls. Absorption of sera with pooled normal kidney cells abrogated reactivity against normal kidney cells but did not decrease reactivity significantly against the renal carcinoma cells. Analyses of sequential serum samples revealed that after nephrectomy in 9 of 10 patients free of tumor but a high risk for recurrence antibody levels decreased or disappeared by 1 year. However, these levels remained elevated in 2 patients who subsequently were found to have occult persistent tumor. Antibody titers in patients with metastatic renal cell carcinoma declined as the disease progressed. Patients with persistently elevated titers had the longest survival. Although the antibodies detected by this assay may not be specific for renal cell carcinoma, their detection may be of clinical significance with regard to the prognosis of the disease."}
{"id": "PMID:470000", "title": "Congenital megaureter.", "content": "Megaureter is the descriptive term applied to the large, wide, dilated and often tortuous ureter, which is usually congenital. Megaureter may result from a number of causes and an accurate diagnostic classification, such as refluxing, obstructed or non-refluxing, non-obstructed, is essential for appropriate management. The 52 consecutive patients with the various forms of megaureter presented typify the various causes of the entity. Surgical intervention virtually is universal and factors bearing upon recoverability and prognosis have been analyzed.", "contents": "Congenital megaureter. Megaureter is the descriptive term applied to the large, wide, dilated and often tortuous ureter, which is usually congenital. Megaureter may result from a number of causes and an accurate diagnostic classification, such as refluxing, obstructed or non-refluxing, non-obstructed, is essential for appropriate management. The 52 consecutive patients with the various forms of megaureter presented typify the various causes of the entity. Surgical intervention virtually is universal and factors bearing upon recoverability and prognosis have been analyzed."}
{"id": "PMID:470001", "title": "The prevalence and significance of Brunn's nests, cystitis cystica and squamous metaplasia in normal bladders.", "content": "The frequency of Brunn's nests, cystitis cystica and squamous metaplasia was studied by multiple histologic sections in 100 grossly normal bladders obtained at postmortem examination. Of the 100 bladders 93 per cent had at least 1 of the 3 proliferative changes. Brunn's nests and cystitis cystica were seen in 89 and 60 per cent of the bladders, respectively, most often at the bladder neck and trigone. Non-keratinizing squamous metaplasia of the vaginal type was seen almost exclusively on the trigone and bladder neck in 46 per cent of the premenopausal and postmenopausal women but in only 7 per cent of the men. There was no satisfactory evidence that inflammation was casually associated with these changes. These findings suggest that Brunn's nests, cystitis cystica and squamous metaplasia of the vaginal type in women are normal variants of bladder urothelium and have no precancerous potential.", "contents": "The prevalence and significance of Brunn's nests, cystitis cystica and squamous metaplasia in normal bladders. The frequency of Brunn's nests, cystitis cystica and squamous metaplasia was studied by multiple histologic sections in 100 grossly normal bladders obtained at postmortem examination. Of the 100 bladders 93 per cent had at least 1 of the 3 proliferative changes. Brunn's nests and cystitis cystica were seen in 89 and 60 per cent of the bladders, respectively, most often at the bladder neck and trigone. Non-keratinizing squamous metaplasia of the vaginal type was seen almost exclusively on the trigone and bladder neck in 46 per cent of the premenopausal and postmenopausal women but in only 7 per cent of the men. There was no satisfactory evidence that inflammation was casually associated with these changes. These findings suggest that Brunn's nests, cystitis cystica and squamous metaplasia of the vaginal type in women are normal variants of bladder urothelium and have no precancerous potential."}
{"id": "PMID:470002", "title": "The survival of patients with bladder tumors treated by surgery: comparative results of an old and a recent series.", "content": "A review of a large number of patients with bladder tumors treated primarily by an operation during approximately 4 decades indicates that survival of patients with papillomas and superficial carcinomas essentially was unchanged. Recently, patients with invasive cancers who have been treated have enjoyed a modest improvement in the 5-year survival rate, which was at least in part the result of a diminished postoperative mortality rate.", "contents": "The survival of patients with bladder tumors treated by surgery: comparative results of an old and a recent series. A review of a large number of patients with bladder tumors treated primarily by an operation during approximately 4 decades indicates that survival of patients with papillomas and superficial carcinomas essentially was unchanged. Recently, patients with invasive cancers who have been treated have enjoyed a modest improvement in the 5-year survival rate, which was at least in part the result of a diminished postoperative mortality rate."}
{"id": "PMID:470004", "title": "Urolithiasis associated with pregnancy.", "content": "Of 34,081 deliveries in a 12-year period 20 were complicated by urinary calculi during the pregnancy. Of the 20 deliveries 3 required intervention either during pregnancy or immediately postpartum. There were no maternal deaths and only 1 stillborn secondary to erythroblastosis. Analysis of symptoms, findings, procedures and outcome is presented in this uncommon but serious problem.", "contents": "Urolithiasis associated with pregnancy. Of 34,081 deliveries in a 12-year period 20 were complicated by urinary calculi during the pregnancy. Of the 20 deliveries 3 required intervention either during pregnancy or immediately postpartum. There were no maternal deaths and only 1 stillborn secondary to erythroblastosis. Analysis of symptoms, findings, procedures and outcome is presented in this uncommon but serious problem."}
{"id": "PMID:470005", "title": "High frequency current effects during transurethral resection.", "content": "Transurethral resection has been simulated electrically to detect the current pathways and locations of heat production inside the patient. Different electrical behavior was noted with instruments using electrically insulated or metal sheaths. Conductive lubricating jelly should be used with the metal sheath and non-conducting jelly should be used with the insulated sheath.", "contents": "High frequency current effects during transurethral resection. Transurethral resection has been simulated electrically to detect the current pathways and locations of heat production inside the patient. Different electrical behavior was noted with instruments using electrically insulated or metal sheaths. Conductive lubricating jelly should be used with the metal sheath and non-conducting jelly should be used with the insulated sheath."}
{"id": "PMID:470006", "title": "Vesicourethral dysfunction in multiple sclerosis.", "content": "Urodynamic evaluation was done 45 times on 41 consecutive patients with multiple sclerosis. Bladder symptoms correlated poorly with any single urodynamic finding and, accordingly, a comprehensive evaluation was necessary to define the underlying pathophysiology. Only 63 per cent of the patients with symptoms of urgency, frequency and urge incontinence actually were found to have uninhibited bladder contractions, while 73 per cent of the patients with obstructive symptoms had detrusor areflexia. Six patients (15 per cent) had a marked change in urodynamic findings upon repeat examination either because of a change in symptomatology or poor response to treatment. An additional 6 patients had vesicoureteral reflux. Bladder symptoms in multiple sclerosis patients should serve more to alert the clinician to the need for urodynamic testing than to mandate specific treatment.", "contents": "Vesicourethral dysfunction in multiple sclerosis. Urodynamic evaluation was done 45 times on 41 consecutive patients with multiple sclerosis. Bladder symptoms correlated poorly with any single urodynamic finding and, accordingly, a comprehensive evaluation was necessary to define the underlying pathophysiology. Only 63 per cent of the patients with symptoms of urgency, frequency and urge incontinence actually were found to have uninhibited bladder contractions, while 73 per cent of the patients with obstructive symptoms had detrusor areflexia. Six patients (15 per cent) had a marked change in urodynamic findings upon repeat examination either because of a change in symptomatology or poor response to treatment. An additional 6 patients had vesicoureteral reflux. Bladder symptoms in multiple sclerosis patients should serve more to alert the clinician to the need for urodynamic testing than to mandate specific treatment."}
{"id": "PMID:470007", "title": "The value of sphincter electromyography in urodynamics.", "content": "Multichannel urodynamic evaluation combined with sphincter electromyography was done on 102 patients. Normal and abnormal activity was defined. When detrusor hyperreflexia occurred in patients with obstruction, idiopathic detrusor hyperreflexia and upper motor neuron disease there was a reflex relaxation of the pelvic floor in 52 per cent of the cases. Electromyographic evidence of dyssynergia was associated with other evidence of sphincter obstruction in 50 per cent of the patients who voided by detrusor contraction and in 9 per cent of the patients who voided by Valsalva's maneuver. A diagnosis of dyssynergia should not be made from the electromyographic evidence alone, especially if the patient strains to void.", "contents": "The value of sphincter electromyography in urodynamics. Multichannel urodynamic evaluation combined with sphincter electromyography was done on 102 patients. Normal and abnormal activity was defined. When detrusor hyperreflexia occurred in patients with obstruction, idiopathic detrusor hyperreflexia and upper motor neuron disease there was a reflex relaxation of the pelvic floor in 52 per cent of the cases. Electromyographic evidence of dyssynergia was associated with other evidence of sphincter obstruction in 50 per cent of the patients who voided by detrusor contraction and in 9 per cent of the patients who voided by Valsalva's maneuver. A diagnosis of dyssynergia should not be made from the electromyographic evidence alone, especially if the patient strains to void."}
{"id": "PMID:470008", "title": "Electrodiagnosis of striated urethral sphincter dysfunction.", "content": "In many centers urethral striated sphincter electromyography has become an essential component of urodynamic testing. The urologist who uses this modality of investigation in an attempt to understand the pathophysiology of striated sphincter dysfunction must have some basic knowledge of electromyography. Attempts have been made to provide such basic information, especially relating to the methods of evaluation and interpretation. We also have incorporated our own views based on our experience with more than 200 subjects with neuropathic vesicourethral dysfunctions.", "contents": "Electrodiagnosis of striated urethral sphincter dysfunction. In many centers urethral striated sphincter electromyography has become an essential component of urodynamic testing. The urologist who uses this modality of investigation in an attempt to understand the pathophysiology of striated sphincter dysfunction must have some basic knowledge of electromyography. Attempts have been made to provide such basic information, especially relating to the methods of evaluation and interpretation. We also have incorporated our own views based on our experience with more than 200 subjects with neuropathic vesicourethral dysfunctions."}
{"id": "PMID:470009", "title": "Low spinal anesthesia for the prevention of autonomic dysreflexia in the spinal cord injury patient.", "content": "Twenty-five patients with traumatic spinal cord injuries above T7 have been managed with low spinal anesthesia during endoscopic urologic procedures. The technique has been completely successful in preventing autonomic dysreflexia and has not been associated with hypotension.", "contents": "Low spinal anesthesia for the prevention of autonomic dysreflexia in the spinal cord injury patient. Twenty-five patients with traumatic spinal cord injuries above T7 have been managed with low spinal anesthesia during endoscopic urologic procedures. The technique has been completely successful in preventing autonomic dysreflexia and has not been associated with hypotension."}
{"id": "PMID:470010", "title": "A one-stage surgical approach to ectopic ureterocele.", "content": "Whenever a ureterectomy for treatment of ectopic ureterocele is appropriate total extravesical excision avoiding a ureteral stump is the preferred approach to avoid a pyoureter or alternating diverticulum and possible surgical complications from an intravesical procedure in small infants and children with large and distorting ectopic ureteroceles.", "contents": "A one-stage surgical approach to ectopic ureterocele. Whenever a ureterectomy for treatment of ectopic ureterocele is appropriate total extravesical excision avoiding a ureteral stump is the preferred approach to avoid a pyoureter or alternating diverticulum and possible surgical complications from an intravesical procedure in small infants and children with large and distorting ectopic ureteroceles."}
{"id": "PMID:470011", "title": "Upper tract management when posterior urethral valve ablation is insufficient.", "content": "Of 105 boys with posterior urethral valves managed during a 10-year period most were managed by primary valve ablation. However, 39 of these boys required concomitant or additional procedures to 71 massively dilated ureters because of azotemia, infection and/or progressive upper tract deterioration. In 17 boys 25 ureters could be reconstructed primarily by varying degrees of ureteral tailoring. When there was severe infection, azotemia and/or doubt as to the function of the affected renal unit, staged reconstruction was initiated by cutaneous ureterostomy. One-fourth of these ureterostomy diverted children died of azotemia despite free urinary drainage. Those who went on to have staged reconstruction, despite multiple surgical procedures, retained intact urinary systems with acceptable function.", "contents": "Upper tract management when posterior urethral valve ablation is insufficient. Of 105 boys with posterior urethral valves managed during a 10-year period most were managed by primary valve ablation. However, 39 of these boys required concomitant or additional procedures to 71 massively dilated ureters because of azotemia, infection and/or progressive upper tract deterioration. In 17 boys 25 ureters could be reconstructed primarily by varying degrees of ureteral tailoring. When there was severe infection, azotemia and/or doubt as to the function of the affected renal unit, staged reconstruction was initiated by cutaneous ureterostomy. One-fourth of these ureterostomy diverted children died of azotemia despite free urinary drainage. Those who went on to have staged reconstruction, despite multiple surgical procedures, retained intact urinary systems with acceptable function."}
{"id": "PMID:470012", "title": "Association of urinary tract infection and reflux with uninhibited bladder contractions and voluntary sphincteric obstruction.", "content": "We studied 53 neurologically normal children with recurrent urinary tract infection who were found to have bladder-sphincter incoordination characterized by voluntary sphincteric constriction during involuntary uninhibited bladder contraction. Increased intravesical pressure was documented during these events and was associated with vesicoureteral reflux in nearly 50 per cent of the children and with abnormalities of the ureteral orifice in 30 per cent of those without reflux. We hypothesize that increased intravesical pressure causes urinary infection in these children and produces a spectrum of intravesical anatomic distortion that predisposes to vesicoureteral reflux. In a prospective uncontrolled study treatment of the uninhibited bladder contractions allowed 58 per cent of the patients to maintain sterile urine without subsequent antimicrobial therapy after cure of the initial infection.", "contents": "Association of urinary tract infection and reflux with uninhibited bladder contractions and voluntary sphincteric obstruction. We studied 53 neurologically normal children with recurrent urinary tract infection who were found to have bladder-sphincter incoordination characterized by voluntary sphincteric constriction during involuntary uninhibited bladder contraction. Increased intravesical pressure was documented during these events and was associated with vesicoureteral reflux in nearly 50 per cent of the children and with abnormalities of the ureteral orifice in 30 per cent of those without reflux. We hypothesize that increased intravesical pressure causes urinary infection in these children and produces a spectrum of intravesical anatomic distortion that predisposes to vesicoureteral reflux. In a prospective uncontrolled study treatment of the uninhibited bladder contractions allowed 58 per cent of the patients to maintain sterile urine without subsequent antimicrobial therapy after cure of the initial infection."}
{"id": "PMID:470014", "title": "Nephrotic syndrome during treatment with alpha-mercaptopropionylglycine.", "content": "We report 2 cases of cystinuria in which a nephrotic syndrome developed during treatment with alpha-mercaptopropionylglycine. This syndrome resolved after withdrawal of the drug and it did not recur when the alpha-mercaptopropionylglycine was given again in lower doses. The hypothesis is made that the nephrotic syndrome was dose-related. The alpha-mercaptopropionylglycine must be used in doses less than 50 mg./kg per day with regular monitoring of 24-hour urinary protein in cystinuric children.", "contents": "Nephrotic syndrome during treatment with alpha-mercaptopropionylglycine. We report 2 cases of cystinuria in which a nephrotic syndrome developed during treatment with alpha-mercaptopropionylglycine. This syndrome resolved after withdrawal of the drug and it did not recur when the alpha-mercaptopropionylglycine was given again in lower doses. The hypothesis is made that the nephrotic syndrome was dose-related. The alpha-mercaptopropionylglycine must be used in doses less than 50 mg./kg per day with regular monitoring of 24-hour urinary protein in cystinuric children."}
{"id": "PMID:470015", "title": "Management of infected polycystic kidneys.", "content": "We report 5 cases of severe uncontrolled infection of polycystic kidneys. Retrograde pyelography antedated infection in 2 of the patients. Difficulty in establishing the diagnosis was demonstrated in all cases. Each patient was treated initially with antibiotics but definitive therapy required an operation in 4 cases.", "contents": "Management of infected polycystic kidneys. We report 5 cases of severe uncontrolled infection of polycystic kidneys. Retrograde pyelography antedated infection in 2 of the patients. Difficulty in establishing the diagnosis was demonstrated in all cases. Each patient was treated initially with antibiotics but definitive therapy required an operation in 4 cases."}
{"id": "PMID:470016", "title": "Acute reversible renal failure secondary to renal candidiasis.", "content": "A diabetic patient presenting with bilateral enlarged kidneys and acute renal failure was found to have renal parenchymal candidiasis without obstruction. Treatment with amphotericin B resulted in return of renal function and decrease in renal size.", "contents": "Acute reversible renal failure secondary to renal candidiasis. A diabetic patient presenting with bilateral enlarged kidneys and acute renal failure was found to have renal parenchymal candidiasis without obstruction. Treatment with amphotericin B resulted in return of renal function and decrease in renal size."}
{"id": "PMID:470017", "title": "Successful treatment of a renal arteriovenous fistula with a Fogarty catheter.", "content": "A large renal arteriovenous fistula developed in a 71-year-old patient after percutaneous needle biopsy. Attempts to control the fistula with autologous clot were unsuccessful. A Fogarty catheter placed fluoroscopically was used to obliterate the feeding vessel. This is the first case reported in which this technique was used to control a renal arteriovenous fistula. This method of treatment offers the advantage of avoiding major surgical intervention with precise control of the fistula.", "contents": "Successful treatment of a renal arteriovenous fistula with a Fogarty catheter. A large renal arteriovenous fistula developed in a 71-year-old patient after percutaneous needle biopsy. Attempts to control the fistula with autologous clot were unsuccessful. A Fogarty catheter placed fluoroscopically was used to obliterate the feeding vessel. This is the first case reported in which this technique was used to control a renal arteriovenous fistula. This method of treatment offers the advantage of avoiding major surgical intervention with precise control of the fistula."}
{"id": "PMID:470018", "title": "Hypertension in childhood from renal vein thrombosis.", "content": "A case is presented of a 1-year-old boy with hypertension resulting from abnormal renin production by the right kidney. The kidney had undergone infarction and atrophy secondary to renal vein thrombosis. The left kidney remained normal despite inferior vena cava thrombosis because of the development of collateral venous circulation. The hypertension was cured by right nephrectomy. The child remains normotensive and with a normal serum creatinine at 1-year followup.", "contents": "Hypertension in childhood from renal vein thrombosis. A case is presented of a 1-year-old boy with hypertension resulting from abnormal renin production by the right kidney. The kidney had undergone infarction and atrophy secondary to renal vein thrombosis. The left kidney remained normal despite inferior vena cava thrombosis because of the development of collateral venous circulation. The hypertension was cured by right nephrectomy. The child remains normotensive and with a normal serum creatinine at 1-year followup."}
{"id": "PMID:470019", "title": "Two-stage procedure for the management of massive hydronephrosis.", "content": "A case of congenital unilateral massive hydronephrosis containing 42.5 l. clear urine is reported. Treatment consisted of a 2-stage procedure that is described. The consequences of sudden decompression, which occurs after a 1-stage operation, are discussed. The rationale of the 2-stage procedure is that gradual decompression helps to restore the normal anatomical and physiological base lines so that the definitive operation of removal of the sac can be accomplished with minimum risk to the patient. This 2-stage technique is a relatively safe procedure based on sound principles.", "contents": "Two-stage procedure for the management of massive hydronephrosis. A case of congenital unilateral massive hydronephrosis containing 42.5 l. clear urine is reported. Treatment consisted of a 2-stage procedure that is described. The consequences of sudden decompression, which occurs after a 1-stage operation, are discussed. The rationale of the 2-stage procedure is that gradual decompression helps to restore the normal anatomical and physiological base lines so that the definitive operation of removal of the sac can be accomplished with minimum risk to the patient. This 2-stage technique is a relatively safe procedure based on sound principles."}
{"id": "PMID:470020", "title": "What about the other kidney?", "content": "Sophisticated diagnostic techniques have made the isolation of renal lesions more accurate. However, it must be remembered that simultaneous bilateral kidney pathology can exist in any patient. The most obvious lesion may not be the most important. Four cases are presented to illustrate this point.", "contents": "What about the other kidney? Sophisticated diagnostic techniques have made the isolation of renal lesions more accurate. However, it must be remembered that simultaneous bilateral kidney pathology can exist in any patient. The most obvious lesion may not be the most important. Four cases are presented to illustrate this point."}
{"id": "PMID:470021", "title": "Renal metastasis of adenocarcinoma of the lung: massive hematuria managed by therapeutic embolization.", "content": "The rare complication of massive hematuria secondary to metastatic infiltrating adenocarcinoma of the lung to the kidney was managed with steel coil therapeutic embolization. The angiographic and pathologic picture as well as the advantages of this therapeutic modality in this clinical setting are discussed.", "contents": "Renal metastasis of adenocarcinoma of the lung: massive hematuria managed by therapeutic embolization. The rare complication of massive hematuria secondary to metastatic infiltrating adenocarcinoma of the lung to the kidney was managed with steel coil therapeutic embolization. The angiographic and pathologic picture as well as the advantages of this therapeutic modality in this clinical setting are discussed."}
{"id": "PMID:470022", "title": "Stones in orthotopic, non-obstructing ureteroceles.", "content": "A case is presented in which stones were found bilaterally in orthotopic, non-obstructing ureteroceles in a child. This is apparently the first reported instance of this condition occurring in the presence of sterile urine. A review of related literature gives clues to the possible etiology of such an occurrence.", "contents": "Stones in orthotopic, non-obstructing ureteroceles. A case is presented in which stones were found bilaterally in orthotopic, non-obstructing ureteroceles in a child. This is apparently the first reported instance of this condition occurring in the presence of sterile urine. A review of related literature gives clues to the possible etiology of such an occurrence."}
{"id": "PMID:470023", "title": "Idiopathic retroperitonial fibrosis misinterpreted as lymphoma by computed tomography.", "content": "Two cases of idiopathic retroperitoneal fibrosis that were mistaken for lymphomas on a computed tomography scan are presented. The similar densities and anatomical appearances on cross section scans of these diseases probably account for this misinterpretation.", "contents": "Idiopathic retroperitonial fibrosis misinterpreted as lymphoma by computed tomography. Two cases of idiopathic retroperitoneal fibrosis that were mistaken for lymphomas on a computed tomography scan are presented. The similar densities and anatomical appearances on cross section scans of these diseases probably account for this misinterpretation."}
{"id": "PMID:470024", "title": "Ureterosigmoidostomy: a 59-year followup and review of long-term urinary diversion.", "content": "This case report documents the efficacy of urinary diversion by ureterosigmoidostomy. Our patient has suffered minimal complications with the added advantage of urinary continence for nearly 60 years. While we would not advocate the Coffey I technique for creation of ureterosigmoidostomy, this case does illustrate the longest survival after any form of diversion with normal urinary function reported in the literature.", "contents": "Ureterosigmoidostomy: a 59-year followup and review of long-term urinary diversion. This case report documents the efficacy of urinary diversion by ureterosigmoidostomy. Our patient has suffered minimal complications with the added advantage of urinary continence for nearly 60 years. While we would not advocate the Coffey I technique for creation of ureterosigmoidostomy, this case does illustrate the longest survival after any form of diversion with normal urinary function reported in the literature."}
{"id": "PMID:470025", "title": "Simultaneous traumatic bladder perforation and disruption of the prostatomembranous urethra.", "content": "The simultaneous occurrence of disruption of the prostatomembranous urethra and traumatic perforation of the bladder is seldom diagnosed before operation for the urethral injury. The presence of posterior disruption can make radiological diagnosis of the concomitant bladder perforation difficult. Lack of a palpable bladder a few hours after urethral injury should arouse suspicion of a combined injury. Careful bladder examination is indicated at the time of operation for all cases of posterior urethral injury to eliminate possible bladder perforations and the significant increase in morbidity and mortality associated with their presence.", "contents": "Simultaneous traumatic bladder perforation and disruption of the prostatomembranous urethra. The simultaneous occurrence of disruption of the prostatomembranous urethra and traumatic perforation of the bladder is seldom diagnosed before operation for the urethral injury. The presence of posterior disruption can make radiological diagnosis of the concomitant bladder perforation difficult. Lack of a palpable bladder a few hours after urethral injury should arouse suspicion of a combined injury. Careful bladder examination is indicated at the time of operation for all cases of posterior urethral injury to eliminate possible bladder perforations and the significant increase in morbidity and mortality associated with their presence."}
{"id": "PMID:470026", "title": "Bilateral testicular dislocation with unilateral rupture.", "content": "A case of bilateral luxation of the testes associated with testicular rupture is presented. Bilateral traumatic dislocation of the testes is uncommon and its association with testicular rupture has not been reported previously. The etiology, classification and treatment of testicular luxation are reviewed briefly. Reports in the literature have indicated initial non-operative management but our case demonstrates the need for early surgical exploration.", "contents": "Bilateral testicular dislocation with unilateral rupture. A case of bilateral luxation of the testes associated with testicular rupture is presented. Bilateral traumatic dislocation of the testes is uncommon and its association with testicular rupture has not been reported previously. The etiology, classification and treatment of testicular luxation are reviewed briefly. Reports in the literature have indicated initial non-operative management but our case demonstrates the need for early surgical exploration."}
{"id": "PMID:470027", "title": "Abdominoscrotal hydrocele in infant with hydronephrosis.", "content": "A case is presented of an unusually large abdominoscrotal hydrocele with secondary left ureterohydronephrosis owing to contiguous pressure. The technique that made the preoperative diagnosis apparent is described.", "contents": "Abdominoscrotal hydrocele in infant with hydronephrosis. A case is presented of an unusually large abdominoscrotal hydrocele with secondary left ureterohydronephrosis owing to contiguous pressure. The technique that made the preoperative diagnosis apparent is described."}
{"id": "PMID:470044", "title": "The increasing importance of polymicrobial bacteremia.", "content": "Polymicrobial bacteremia increased from 6% in 1970 to 13% in 1975 in patients with bloodstream infections. This type of serious infection most commonly complicated genitourinary (27%) and gastrointestinal (26%) conditions, frequently followed invasive procedures (68%), was more common in hospitalized patients (73%), and was often associated with malignancies (25%) or immunosuppressive or cancer chemotherapy (34%). Although polymicrobial endocarditis was more commonly encountered in recent years, this infection accounted for only 4.5% of patients with multiple organism bacteremias. Gram-negative aerobic bacteria were isolated from 62% and anaerobic bacteria in 39% of patients with polymicrobial bacteremia compared with 37% and 14%, respectively, in patients with monomicrobial bacteremia. In the 88 patients with polymicrobial bacteremia, the mortality was 44.5%, compared with 18.0% in patients with monomicrobial bacteremia.", "contents": "The increasing importance of polymicrobial bacteremia. Polymicrobial bacteremia increased from 6% in 1970 to 13% in 1975 in patients with bloodstream infections. This type of serious infection most commonly complicated genitourinary (27%) and gastrointestinal (26%) conditions, frequently followed invasive procedures (68%), was more common in hospitalized patients (73%), and was often associated with malignancies (25%) or immunosuppressive or cancer chemotherapy (34%). Although polymicrobial endocarditis was more commonly encountered in recent years, this infection accounted for only 4.5% of patients with multiple organism bacteremias. Gram-negative aerobic bacteria were isolated from 62% and anaerobic bacteria in 39% of patients with polymicrobial bacteremia compared with 37% and 14%, respectively, in patients with monomicrobial bacteremia. In the 88 patients with polymicrobial bacteremia, the mortality was 44.5%, compared with 18.0% in patients with monomicrobial bacteremia."}
{"id": "PMID:470045", "title": "Prenatal diagnosis of propionic acidemia.", "content": "Prenatal diagnosis of a fetus with propionic acidemia has been accomplished by the detection of methylcitrate, a unique metabolite, in the amniotic fluid by liquid partition chromatography and gas chromatography-mass spectrometry. The diagnosis was confirmed by demonstration of deficient activity of propionyl-CoA carboxylase in cells cultured from the amniotic fluid and in fetal tissues. In two subsequent pregnancies, methylcitrate was not present in amniotic fluid. Enzyme assay indicated that one fetus was heterozygous and the other was normal, and healthy infants were born at term. The analysis of organic acids in amniotic fluid permits very rapid prenatal diagnosis, usually within 48 hours after obtaining the fluid. It may provide a general method in conditions in which an unusual metabolite can be identified.", "contents": "Prenatal diagnosis of propionic acidemia. Prenatal diagnosis of a fetus with propionic acidemia has been accomplished by the detection of methylcitrate, a unique metabolite, in the amniotic fluid by liquid partition chromatography and gas chromatography-mass spectrometry. The diagnosis was confirmed by demonstration of deficient activity of propionyl-CoA carboxylase in cells cultured from the amniotic fluid and in fetal tissues. In two subsequent pregnancies, methylcitrate was not present in amniotic fluid. Enzyme assay indicated that one fetus was heterozygous and the other was normal, and healthy infants were born at term. The analysis of organic acids in amniotic fluid permits very rapid prenatal diagnosis, usually within 48 hours after obtaining the fluid. It may provide a general method in conditions in which an unusual metabolite can be identified."}
{"id": "PMID:470046", "title": "The autopsy. Past, present, and future.", "content": "The autopsy appears to be on the way to assuming an increasingly important role in patient care, continuing education, education of medical students and house staff, research in environmental pathology, and more fundamental aspects of disease. This trend needs to be nurtured. Not only pathologists but all physicians need to plan carefully to utilize autopsies to the fullest possible extent. Interest will continue to grow only if autopsies are performed or are closely supervised by highly motivated and experienced pathologists asking critical questions of current medical, scientific, and social concern. New, imaginative approaches are needed to develop the data necessary to address these questions.", "contents": "The autopsy. Past, present, and future. The autopsy appears to be on the way to assuming an increasingly important role in patient care, continuing education, education of medical students and house staff, research in environmental pathology, and more fundamental aspects of disease. This trend needs to be nurtured. Not only pathologists but all physicians need to plan carefully to utilize autopsies to the fullest possible extent. Interest will continue to grow only if autopsies are performed or are closely supervised by highly motivated and experienced pathologists asking critical questions of current medical, scientific, and social concern. New, imaginative approaches are needed to develop the data necessary to address these questions."}
{"id": "PMID:470047", "title": "Hypoxemia after thoracentesis. A predictable and treatable condition.", "content": "Hypoxemia was observed at 20 minutes and two hours postprocedure in 15 patients who had a thoracentesis. The hypoxemia resolved after 24 hours. No effect on pH, PaCO2, or bicarbonate ion was observed. The degree of hypoxemia was correlated directly with the volume of fluid removed. Oxygen therapy was found to reverse this effect. Hypoxemia that follows thoracentesis may be caused by several factors, the most important being frank pulmonary edema.", "contents": "Hypoxemia after thoracentesis. A predictable and treatable condition. Hypoxemia was observed at 20 minutes and two hours postprocedure in 15 patients who had a thoracentesis. The hypoxemia resolved after 24 hours. No effect on pH, PaCO2, or bicarbonate ion was observed. The degree of hypoxemia was correlated directly with the volume of fluid removed. Oxygen therapy was found to reverse this effect. Hypoxemia that follows thoracentesis may be caused by several factors, the most important being frank pulmonary edema."}
{"id": "PMID:470048", "title": "Limitatoins of surgical methods of pericardial drainage. Echocardiographic observations.", "content": "To assess the efficacy of surgical methods of pericardial drainage in preventing the accumulation of pericardial effusion, we performed M-mode and cross-sectional echocardiograms in 26 patients before and one week after the creation of a pericardial window or leaving the pericardium open after cardiac surgery. A pericardial window was created in six patients (group 1) who had pericardial effusions. Group 2 consisted of 20 patients who had heart surgery. Echocardiography disclosed moderate or large pericardial effusions preoperatively in all group 1 patients but in one of the group 2 patients. Following operation, three of the group 1 patients had complete clearing of pericardial effusion, while three showed reaccumulation. In group 2, thirteen patients had either no effusion or a small effusion. Seven patients had moderate or large pericardial effusions that were located posterior to the heart; only one had an anterior pericardial effusion. Cross-sectional echocardiography showed posterior loculation of effusion in six of the seven patients. Pericardial drainage is not always effective in preventing accumulation of pericardial effusions.", "contents": "Limitatoins of surgical methods of pericardial drainage. Echocardiographic observations. To assess the efficacy of surgical methods of pericardial drainage in preventing the accumulation of pericardial effusion, we performed M-mode and cross-sectional echocardiograms in 26 patients before and one week after the creation of a pericardial window or leaving the pericardium open after cardiac surgery. A pericardial window was created in six patients (group 1) who had pericardial effusions. Group 2 consisted of 20 patients who had heart surgery. Echocardiography disclosed moderate or large pericardial effusions preoperatively in all group 1 patients but in one of the group 2 patients. Following operation, three of the group 1 patients had complete clearing of pericardial effusion, while three showed reaccumulation. In group 2, thirteen patients had either no effusion or a small effusion. Seven patients had moderate or large pericardial effusions that were located posterior to the heart; only one had an anterior pericardial effusion. Cross-sectional echocardiography showed posterior loculation of effusion in six of the seven patients. Pericardial drainage is not always effective in preventing accumulation of pericardial effusions."}
{"id": "PMID:470051", "title": "Roentgenographic evaluation of cervical spine trauma.", "content": "A practical approach to the roentgenographic assessment of a patient suspected of having a cervical spine injury is described. Once positioned, the patient is not moved until substantial bony injury has been excluded. The major roentgenographic signs with differential diagnosis are briefly discussed using some illustrative roentgenograms.", "contents": "Roentgenographic evaluation of cervical spine trauma. A practical approach to the roentgenographic assessment of a patient suspected of having a cervical spine injury is described. Once positioned, the patient is not moved until substantial bony injury has been excluded. The major roentgenographic signs with differential diagnosis are briefly discussed using some illustrative roentgenograms."}
{"id": "PMID:470065", "title": "Continuing medical education.", "content": "1. Continuing medical education is unquestionably a worthwhile goal. Knowledge or exposure to information, however, does not ensure competence in medical care; nevertheless, without knowledge there is no possibility of true competence. 2. Continuing medical education should not be judged by recall knowledge but should ensure exposure to current and acceptable medical knowledge. 3. Because all licensed physicians have unrestricted licenses regardless of their specialty, they all should benefit from a comprehensive type of CME. 4. An open-book examination with reference sources appended to the questions would expose the physician to current and acceptable medical knowledge. 5. Recall types of examinations have no place in practical CME.", "contents": "Continuing medical education. 1. Continuing medical education is unquestionably a worthwhile goal. Knowledge or exposure to information, however, does not ensure competence in medical care; nevertheless, without knowledge there is no possibility of true competence. 2. Continuing medical education should not be judged by recall knowledge but should ensure exposure to current and acceptable medical knowledge. 3. Because all licensed physicians have unrestricted licenses regardless of their specialty, they all should benefit from a comprehensive type of CME. 4. An open-book examination with reference sources appended to the questions would expose the physician to current and acceptable medical knowledge. 5. Recall types of examinations have no place in practical CME."}
{"id": "PMID:470066", "title": "Serum alkaline phosphatase determination. Value in the staging of advanced breast cancer.", "content": "A retrospective study of 146 patients with metastatic disease was undertaken to verify the clinical impression that radionuclide scanning rarely, if ever, discloses hepatic metastases in breast cancer patients with normal serum alkaline phosphatase (AP) levels. Only two of 39 patients with abnormal liver scans had normal AP levels, and we conclude that liver scans are not necessary as a routine screening method for liver metastases when the AP level is normal. In contrast AP levels were not predictive of bone scan results. All patients with a twofold or greater elevation of the AP level had abnormal bone, liver, or bone and liver scans. Routine AP determinations provide accurate staging information, and their proper use can decrease the cost of initial and follow-up examination of patients with breast cancer.", "contents": "Serum alkaline phosphatase determination. Value in the staging of advanced breast cancer. A retrospective study of 146 patients with metastatic disease was undertaken to verify the clinical impression that radionuclide scanning rarely, if ever, discloses hepatic metastases in breast cancer patients with normal serum alkaline phosphatase (AP) levels. Only two of 39 patients with abnormal liver scans had normal AP levels, and we conclude that liver scans are not necessary as a routine screening method for liver metastases when the AP level is normal. In contrast AP levels were not predictive of bone scan results. All patients with a twofold or greater elevation of the AP level had abnormal bone, liver, or bone and liver scans. Routine AP determinations provide accurate staging information, and their proper use can decrease the cost of initial and follow-up examination of patients with breast cancer."}
{"id": "PMID:470067", "title": "Risk of vascular disease in women. Smoking, oral contraceptives, noncontraceptive estrogens, and other factors.", "content": "We investigated the relation in women of various factors to risk of myocardial infarction, subarachnoid hemorrhage, other strokes, and venous thromboembolism. Smoking significantly increased risk of all four diseases, whereas oral contraceptive use was associated with an increase only in risk of subarachnoid hemorrhage and venous thromboembolism. Use of noncontraceptive estrogens was not associated with increased risk of any of these diseases. Hypertension, hypercholesterolemia, obesity, gallbladder disease, and nondrinking of alcohol were all associated with increased risk of myocardial infarction, whereas only hypertension and hypercholesterolemia were associated with increased risk of other strokes. Cigarette smoking was overwhelmingly the most important risk factor for vascular disease in women. Smoking should be considered a contraindication to oral contraceptive use, or at the very least, women wishing to use oral contraceptives should be strongly urged not to smoke.", "contents": "Risk of vascular disease in women. Smoking, oral contraceptives, noncontraceptive estrogens, and other factors. We investigated the relation in women of various factors to risk of myocardial infarction, subarachnoid hemorrhage, other strokes, and venous thromboembolism. Smoking significantly increased risk of all four diseases, whereas oral contraceptive use was associated with an increase only in risk of subarachnoid hemorrhage and venous thromboembolism. Use of noncontraceptive estrogens was not associated with increased risk of any of these diseases. Hypertension, hypercholesterolemia, obesity, gallbladder disease, and nondrinking of alcohol were all associated with increased risk of myocardial infarction, whereas only hypertension and hypercholesterolemia were associated with increased risk of other strokes. Cigarette smoking was overwhelmingly the most important risk factor for vascular disease in women. Smoking should be considered a contraindication to oral contraceptive use, or at the very least, women wishing to use oral contraceptives should be strongly urged not to smoke."}
{"id": "PMID:470068", "title": "Erythema and conjunctivitis. Outbreak caused by inadvertent exposure to ultraviolet light.", "content": "During August to November 1977, fifty-eight episodes of erythema or conjunctivitis or both occurred among nursing-service employees at a large state hospital. Epidemiologic investigation implicated an ultraviolet-light-producing germicidal bulb in a nursing station's desk lamp as the causative agent. Removal of the lamp from the nursing station controlled the outbreak. Analysis of the spectral irradiance from the bulb suggested that the National Institute of Occupational Safety and Health exposure standards were exceeded in five to 20 minutes, depending on the individual's distance from the lamp.", "contents": "Erythema and conjunctivitis. Outbreak caused by inadvertent exposure to ultraviolet light. During August to November 1977, fifty-eight episodes of erythema or conjunctivitis or both occurred among nursing-service employees at a large state hospital. Epidemiologic investigation implicated an ultraviolet-light-producing germicidal bulb in a nursing station's desk lamp as the causative agent. Removal of the lamp from the nursing station controlled the outbreak. Analysis of the spectral irradiance from the bulb suggested that the National Institute of Occupational Safety and Health exposure standards were exceeded in five to 20 minutes, depending on the individual's distance from the lamp."}
{"id": "PMID:470070", "title": "Health effects of urban air pollution. Special consideration of areas at 1,500 m and above.", "content": "Urban air pollution, when sufficiently severe, can cause death in humans; these deaths occur preponderantly among those with increased susceptibility. Pollution--carbon monoxide and oxidants in particular--is capable of aggravating preexisting chronic heart and lung diseases. Pollution and tobacco (especially cigarette) smoke act synergistically in a causative role in chronic bronchitis and in an aggravating role in emphysema. Susceptibility to the effects of urban air pollution varies widely, with the most susceptible being fetuses, the newborn, the elderly, the infirm, those with chronic heart and lung diseases, and those who smoke. The carbon monoxide standards for sea level are probably too lenient for an altitude of 1,500 m and above. Using a broad definition of health, which takes into account concepts such as quality of life and social and mental well-being, few metropolitan residents would deny that their health is compromised by air that is malodorous, that irritates mucous membranes, and that obscures beautiful scenery.", "contents": "Health effects of urban air pollution. Special consideration of areas at 1,500 m and above. Urban air pollution, when sufficiently severe, can cause death in humans; these deaths occur preponderantly among those with increased susceptibility. Pollution--carbon monoxide and oxidants in particular--is capable of aggravating preexisting chronic heart and lung diseases. Pollution and tobacco (especially cigarette) smoke act synergistically in a causative role in chronic bronchitis and in an aggravating role in emphysema. Susceptibility to the effects of urban air pollution varies widely, with the most susceptible being fetuses, the newborn, the elderly, the infirm, those with chronic heart and lung diseases, and those who smoke. The carbon monoxide standards for sea level are probably too lenient for an altitude of 1,500 m and above. Using a broad definition of health, which takes into account concepts such as quality of life and social and mental well-being, few metropolitan residents would deny that their health is compromised by air that is malodorous, that irritates mucous membranes, and that obscures beautiful scenery."}
{"id": "PMID:470086", "title": "A field study of the stress response syndrome. Young women after hysterectomy.", "content": "The symptom pattern previously delineated as the stress response syndrome in a mental health setting was hypothesized to be useful in conceptualizing reactions to a traumatic event in a nonpsychiatric patient population. The experience of loss resulting from nonelective hysterectomy for benign disease in women of childbearing age was selected as a relevant field study model. Twenty-eight women were studied one year after hysterectomy, using extensive psychological interviewing by women clinicians and experiential rating scales. Twelve subjects had a mild stress response syndrome, and five subjects had a serious level of intrusive and avoidant symptoms. Increasing severity of response was associated with persisting child-wish, deterioration in sexual functioning, and change in self-concept. Women who did well postoperatively generally had no future wish for children and were actively committed to achievement outside of the home.", "contents": "A field study of the stress response syndrome. Young women after hysterectomy. The symptom pattern previously delineated as the stress response syndrome in a mental health setting was hypothesized to be useful in conceptualizing reactions to a traumatic event in a nonpsychiatric patient population. The experience of loss resulting from nonelective hysterectomy for benign disease in women of childbearing age was selected as a relevant field study model. Twenty-eight women were studied one year after hysterectomy, using extensive psychological interviewing by women clinicians and experiential rating scales. Twelve subjects had a mild stress response syndrome, and five subjects had a serious level of intrusive and avoidant symptoms. Increasing severity of response was associated with persisting child-wish, deterioration in sexual functioning, and change in self-concept. Women who did well postoperatively generally had no future wish for children and were actively committed to achievement outside of the home."}
{"id": "PMID:470087", "title": "Psychological coping mechanisms and survival time in metastatic breast cancer.", "content": "Thirty-five women with metastatic breast cancer received a battery of baseline psychological tests; results were correlated with length of survival. Patients who died in less than one year from baseline were categorized as short-term survivors, while patients who lived for one year or longer were assigned to the long-term survivor group. The long-term survivors were more symptomatic overall, with particular elevations on measures of anxiety and alienation, and substantially higher levels of dysphoric mood (eg, depression, guilt) than the short-term survivors. Short-term survivors revealed significantly lower levels of hostility, with higher levels of positive mood. Treating oncologists perceived the long-term survivors to show significantly poorer adjustment to their illnesses than the short-term survivors, and an interviewer's ratings indicated that long-term survivors had significantly poorer attitudes toward their physicians. Measures of clinical status and demographic data revealed few differences between the two groups.", "contents": "Psychological coping mechanisms and survival time in metastatic breast cancer. Thirty-five women with metastatic breast cancer received a battery of baseline psychological tests; results were correlated with length of survival. Patients who died in less than one year from baseline were categorized as short-term survivors, while patients who lived for one year or longer were assigned to the long-term survivor group. The long-term survivors were more symptomatic overall, with particular elevations on measures of anxiety and alienation, and substantially higher levels of dysphoric mood (eg, depression, guilt) than the short-term survivors. Short-term survivors revealed significantly lower levels of hostility, with higher levels of positive mood. Treating oncologists perceived the long-term survivors to show significantly poorer adjustment to their illnesses than the short-term survivors, and an interviewer's ratings indicated that long-term survivors had significantly poorer attitudes toward their physicians. Measures of clinical status and demographic data revealed few differences between the two groups."}
{"id": "PMID:470088", "title": "Postoperative adjuvant chemotherapy with fluorouracil, doxorubicin, cyclophosphamide, and BCG vaccine. A follow-up report.", "content": "Two hundred twenty-two patients with stage II or III breast cancer following regional therapy were treated with a combination of fluorouracil, doxorubicin hydrochloride (Adrimycin), cyclophosphamide, and BCG vaccine. At 54 months of study (median follow-up, 30 months), the estimated proportions remaining disease-free two and three years after surgery were 83%and 78%, respectively, in the chemotherapy group and 64% and 55%, respectively, in 151 historical control patients. Estimated two- and three-year survival rates were 93% and 89%, respectively, in the chemotherapy group and 84% and 58%, respectively, in the control patients. Congestive heart failure has developed in three patients, possibly related to the use of doxorubicin. Adjuvant chemotherapy with these drugs was effective in prolonging the disease-free interval and survival of patients irrespective of menopausal status, degree of nodal involvement, or stage of the disease.", "contents": "Postoperative adjuvant chemotherapy with fluorouracil, doxorubicin, cyclophosphamide, and BCG vaccine. A follow-up report. Two hundred twenty-two patients with stage II or III breast cancer following regional therapy were treated with a combination of fluorouracil, doxorubicin hydrochloride (Adrimycin), cyclophosphamide, and BCG vaccine. At 54 months of study (median follow-up, 30 months), the estimated proportions remaining disease-free two and three years after surgery were 83%and 78%, respectively, in the chemotherapy group and 64% and 55%, respectively, in 151 historical control patients. Estimated two- and three-year survival rates were 93% and 89%, respectively, in the chemotherapy group and 84% and 58%, respectively, in the control patients. Congestive heart failure has developed in three patients, possibly related to the use of doxorubicin. Adjuvant chemotherapy with these drugs was effective in prolonging the disease-free interval and survival of patients irrespective of menopausal status, degree of nodal involvement, or stage of the disease."}
{"id": "PMID:470089", "title": "Viral hepatitis associated with day-care centers.", "content": "From September 1976 through March 1978, we investigated 11 outbreaks of non-B viral hepatitis associated with Louisiana day-care centers. The outbreaks included 168 cases, most of which were erroneously considered \"sporadic\" cases of non-B viral hepatitis prior to the investigations. Thirteen percent of all non-B viral hepatitis cases reported in the New Orleans metropolitan area during 1977 were associated with one of the outbreaks. Most of the cases in each outbreak and 85% overall were in older, usually adult, contacts of children attending the day-care centers. Within the household, parents appeared to be at greatest risk, particularly those who had 1- to 2-year-old children in the day-care center. Day-care center outbreaks of non-B hepatitis are easily overlooked and may be more widespread than is currently appreciated.", "contents": "Viral hepatitis associated with day-care centers. From September 1976 through March 1978, we investigated 11 outbreaks of non-B viral hepatitis associated with Louisiana day-care centers. The outbreaks included 168 cases, most of which were erroneously considered \"sporadic\" cases of non-B viral hepatitis prior to the investigations. Thirteen percent of all non-B viral hepatitis cases reported in the New Orleans metropolitan area during 1977 were associated with one of the outbreaks. Most of the cases in each outbreak and 85% overall were in older, usually adult, contacts of children attending the day-care centers. Within the household, parents appeared to be at greatest risk, particularly those who had 1- to 2-year-old children in the day-care center. Day-care center outbreaks of non-B hepatitis are easily overlooked and may be more widespread than is currently appreciated."}
{"id": "PMID:470098", "title": "Effect of allopurinol (zyloric) on patients undergoing open heart surgery.", "content": "We observed previously that allopurinol, which was used for the treatment of gout, had a life saving effect after experiment on traumatic shock rats and hemorrhagic shock rabbits. To evaluate the effect of allopurinol fifteen patients undergoing open heart surgery who were considered to have similar metabolic derangement in shock patient were examined. Allopurinol was given orally 2 mg per kg body weight twice before the start of nitrous oxide, oxygen and halothane anesthesia. In the control group of ten patients who were not treated with allopurinol, serum uric acid increased, the lactate/pyruvate ratio rose and beta-glucuronidase activity increased respectively after open heart surgery as in shock. But the metabolic changes of the fifteen patients pretreated with allopurinol were less significant, although same tendency was observed. The heart beat of all patients except one case in the allopurinol group, started spontaneously after extracorporeal circulation without using DC counter shock. In the control group all patients needed DC counter shock. We concluded that allopurinol was effective in preventing damage of cellular structures and derangements of metabolism of patients undergoing open heart surgery.", "contents": "Effect of allopurinol (zyloric) on patients undergoing open heart surgery. We observed previously that allopurinol, which was used for the treatment of gout, had a life saving effect after experiment on traumatic shock rats and hemorrhagic shock rabbits. To evaluate the effect of allopurinol fifteen patients undergoing open heart surgery who were considered to have similar metabolic derangement in shock patient were examined. Allopurinol was given orally 2 mg per kg body weight twice before the start of nitrous oxide, oxygen and halothane anesthesia. In the control group of ten patients who were not treated with allopurinol, serum uric acid increased, the lactate/pyruvate ratio rose and beta-glucuronidase activity increased respectively after open heart surgery as in shock. But the metabolic changes of the fifteen patients pretreated with allopurinol were less significant, although same tendency was observed. The heart beat of all patients except one case in the allopurinol group, started spontaneously after extracorporeal circulation without using DC counter shock. In the control group all patients needed DC counter shock. We concluded that allopurinol was effective in preventing damage of cellular structures and derangements of metabolism of patients undergoing open heart surgery."}
{"id": "PMID:470099", "title": "Rate related block in the canine His-Purkinje system after ligation of the anterior septal artery--observation by His bundle electrocardiography.", "content": "In order to investigate the pathophysiology of tachycardia and bradycardia dependent block in the His-Purkinje system, anterior septal artery was ligated and the effects of vagal stimulation and atrial pacing were serially checked in 26 dogs. Tachycardia dependent intra-His bundle block or H-V blocks developed in all cases following ligation of the anterior septal artery and bradycardia dependent block ensued in 3 cases. The latter appeared only in the form of split His bundle or lengthening of the H-V intervals. Spontaneous second or third degree AV block appeared thereafter. Bundle branch block appeared in 19 cases after ligation of the artery. Rate dependent bundle branch block was observed before the development of stable bundle branch block. Frequently, a very long pause was necessary to produce bradycardia dependent block. On the other hand, bradycardia dependent H-V blocks by vagal stimulation were not necessarily dependent upon cardiac cycle length alone. Tachycardia dependent paroxysmal A-V blocks were induced in 7 cases by atrial pacing, soon before or after the onset of spontaneous second degree A-V block. In addition, this block was induced in the very early phase after ligation of the artery in one case. This repetitive intra-His bundle block or H-V block was induced not only by progressive increase in pacing rate but also by gradual decrease in pacing rate after a Wenckebach type of second degree A-H block.", "contents": "Rate related block in the canine His-Purkinje system after ligation of the anterior septal artery--observation by His bundle electrocardiography. In order to investigate the pathophysiology of tachycardia and bradycardia dependent block in the His-Purkinje system, anterior septal artery was ligated and the effects of vagal stimulation and atrial pacing were serially checked in 26 dogs. Tachycardia dependent intra-His bundle block or H-V blocks developed in all cases following ligation of the anterior septal artery and bradycardia dependent block ensued in 3 cases. The latter appeared only in the form of split His bundle or lengthening of the H-V intervals. Spontaneous second or third degree AV block appeared thereafter. Bundle branch block appeared in 19 cases after ligation of the artery. Rate dependent bundle branch block was observed before the development of stable bundle branch block. Frequently, a very long pause was necessary to produce bradycardia dependent block. On the other hand, bradycardia dependent H-V blocks by vagal stimulation were not necessarily dependent upon cardiac cycle length alone. Tachycardia dependent paroxysmal A-V blocks were induced in 7 cases by atrial pacing, soon before or after the onset of spontaneous second degree A-V block. In addition, this block was induced in the very early phase after ligation of the artery in one case. This repetitive intra-His bundle block or H-V block was induced not only by progressive increase in pacing rate but also by gradual decrease in pacing rate after a Wenckebach type of second degree A-H block."}
{"id": "PMID:470101", "title": "Epidemiology of rheumatic fever in Japan.", "content": "The number of patients with the first attack of rheumatic fever reached a peak in 1960, decreased remarkably until 1968, then has remained unchanged for the last nine years. Approximately 10% of rheumatic patients still develop recurrences.", "contents": "Epidemiology of rheumatic fever in Japan. The number of patients with the first attack of rheumatic fever reached a peak in 1960, decreased remarkably until 1968, then has remained unchanged for the last nine years. Approximately 10% of rheumatic patients still develop recurrences."}
{"id": "PMID:470104", "title": "Clinical aspects of nonrheumatic myocarditis in children.", "content": "Sixty-eight patients of clinically diagnosed myocarditis, 0--15 years of age, were followed up and analyzed. Forty (58.8%) were males. The majority were older than 5 years. Clinical courses were rather mild, chronic and self-limiting at large. Only 1 case had a relation to chronic cariomyopathy. Exertional symptoms (chest pain, chest distress, syncope) were seen in 25 (36.8%). ECG changes were very common: the majority were nonspecific ST elevation, depression or both, mainly in leads II, III, V5 and V6. Positive Master' test, prolonged QTc, widened mean spatial QRS-T angle and various arrhythmias were also observed. Cardiac performance, estimated by echocardiogram and phono-mechanocardiogram was lowered in 41 (60.3%). Large IV sound and large A wave in apexcardiogram were also frequently found. All but 3 patients showed continuous elevation of serum enzymes, namely, LDH, LDH-1/LDH-2, CPK, CPK-MB, HBD and GOT. Etiological evidences were obtained by serological study in 11 cases (16.2%): 2 of Coxsackie B-1, 3 of Coxsackie B-2, 1 of Coxsackie B-4, 2 of mycoplasma pneumoniae, 1 of cytomegalovirus, 1 of ECHO-7 and 1 of rubella. We proposed a criteria for diagnosis of myocarditis as follows: (1) Exertional symptoms. (2) ECG findings. (3) Serum enzyme abnormality. (4) Lowered cardiac performance. (5) Cardiomegaly. (6) Changing character of all signs and symptoms.", "contents": "Clinical aspects of nonrheumatic myocarditis in children. Sixty-eight patients of clinically diagnosed myocarditis, 0--15 years of age, were followed up and analyzed. Forty (58.8%) were males. The majority were older than 5 years. Clinical courses were rather mild, chronic and self-limiting at large. Only 1 case had a relation to chronic cariomyopathy. Exertional symptoms (chest pain, chest distress, syncope) were seen in 25 (36.8%). ECG changes were very common: the majority were nonspecific ST elevation, depression or both, mainly in leads II, III, V5 and V6. Positive Master' test, prolonged QTc, widened mean spatial QRS-T angle and various arrhythmias were also observed. Cardiac performance, estimated by echocardiogram and phono-mechanocardiogram was lowered in 41 (60.3%). Large IV sound and large A wave in apexcardiogram were also frequently found. All but 3 patients showed continuous elevation of serum enzymes, namely, LDH, LDH-1/LDH-2, CPK, CPK-MB, HBD and GOT. Etiological evidences were obtained by serological study in 11 cases (16.2%): 2 of Coxsackie B-1, 3 of Coxsackie B-2, 1 of Coxsackie B-4, 2 of mycoplasma pneumoniae, 1 of cytomegalovirus, 1 of ECHO-7 and 1 of rubella. We proposed a criteria for diagnosis of myocarditis as follows: (1) Exertional symptoms. (2) ECG findings. (3) Serum enzyme abnormality. (4) Lowered cardiac performance. (5) Cardiomegaly. (6) Changing character of all signs and symptoms."}
{"id": "PMID:470105", "title": "Experimental cryptococcal myocarditis.", "content": "Rabbits and rats developed myocarditis on the 16th, 30th, and 60th day after intrarenal inoculation with Cryptococcus neoformans. The cardiac lesions consist of focal necrosis with infiltrations of small round cells in the myocardium. Cryptococcal antigens were demonstrated by a direct immunofluorescent antibody method in the damaged myocardial lesions. Cryptococcus neoformans itself was found by periodic acid-Schiff stain in the damaged myocardial lesions.", "contents": "Experimental cryptococcal myocarditis. Rabbits and rats developed myocarditis on the 16th, 30th, and 60th day after intrarenal inoculation with Cryptococcus neoformans. The cardiac lesions consist of focal necrosis with infiltrations of small round cells in the myocardium. Cryptococcal antigens were demonstrated by a direct immunofluorescent antibody method in the damaged myocardial lesions. Cryptococcus neoformans itself was found by periodic acid-Schiff stain in the damaged myocardial lesions."}
{"id": "PMID:470113", "title": "Rheumatic heart disease in childhood: comparative study between Japan and Thailand.", "content": "This report showed the comparative study of the rheumatic fever and rheumatic heart disease in Japan and Thailand. At first, there is remarkable difference about the incidence of the rheumatic heart disease. The incidence of the rheumatic heart disease in Thailand was one hundred times that in Japan. Secondly, there is particular difference about the course of mitral stenosis in childhood. In Japan common pattern of rheumatic heart disease was slight mitral insufficiency and mitral stenosis was seldom. In Thailand mitral stenosis was observed in 5% on rheumatic heart disease, and mitral stenosis developed early following an attack of rheumatic fever. These differences were supposed due to the socio-economic state in each country.", "contents": "Rheumatic heart disease in childhood: comparative study between Japan and Thailand. This report showed the comparative study of the rheumatic fever and rheumatic heart disease in Japan and Thailand. At first, there is remarkable difference about the incidence of the rheumatic heart disease. The incidence of the rheumatic heart disease in Thailand was one hundred times that in Japan. Secondly, there is particular difference about the course of mitral stenosis in childhood. In Japan common pattern of rheumatic heart disease was slight mitral insufficiency and mitral stenosis was seldom. In Thailand mitral stenosis was observed in 5% on rheumatic heart disease, and mitral stenosis developed early following an attack of rheumatic fever. These differences were supposed due to the socio-economic state in each country."}
{"id": "PMID:470139", "title": "Ultrasonodiagnosis of subclinical heart failure by increasing afterload with angiotensin II.", "content": "In an attempt to diagnose latent heart failure, a transient increase of afterload to left ventricle was produced by angiotensin II, and the ventricular movement was assessed by echocardiography. Angiotensin II was administered in a dose of 0.075 micrograms/kg body weight. Blood pressure was elevated by 40.5 +/- 14.5 mmHg in 38 cases with latent heart failure and by 41.2 +/- 4.5 mmHg in 30 normal subjects. Yet, only in the former group, posterior wall excursion of left ventricle reduced from 11.4 +/- 2.4 mm to 7.3 +/- 2.3 mm and mean posterior wall velocity from 41.4 +/- 10.1mm/sec to 26.5 +/- 8.8 mm/sec. The rates of these reductions were inversely correlated to the rate of elevation of left ventricular endodiastolic pressure as determined in 5 cases by cardiac catheterization. There was no change in 30 subjects. The data indicate the usefulness of angiotensin-induced echocardiographic changes in detecting latent cadiac failure.", "contents": "Ultrasonodiagnosis of subclinical heart failure by increasing afterload with angiotensin II. In an attempt to diagnose latent heart failure, a transient increase of afterload to left ventricle was produced by angiotensin II, and the ventricular movement was assessed by echocardiography. Angiotensin II was administered in a dose of 0.075 micrograms/kg body weight. Blood pressure was elevated by 40.5 +/- 14.5 mmHg in 38 cases with latent heart failure and by 41.2 +/- 4.5 mmHg in 30 normal subjects. Yet, only in the former group, posterior wall excursion of left ventricle reduced from 11.4 +/- 2.4 mm to 7.3 +/- 2.3 mm and mean posterior wall velocity from 41.4 +/- 10.1mm/sec to 26.5 +/- 8.8 mm/sec. The rates of these reductions were inversely correlated to the rate of elevation of left ventricular endodiastolic pressure as determined in 5 cases by cardiac catheterization. There was no change in 30 subjects. The data indicate the usefulness of angiotensin-induced echocardiographic changes in detecting latent cadiac failure."}
{"id": "PMID:470140", "title": "Presystolic pulmonary valve opening in constrictive pericarditis.", "content": "Right heart pressure tracings were studied in 5 cases with constrictive pericarditis. The pressure of the A-wave in the right atrium of these cases was higher than the pulmonary arterial diastolic pressure. In addition, a presystolic wave appeared in the pulmonary arterial and the right ventricular pressure tracings, in coincidence with atrial systole. The pressure of this presystolic wave was similar to that of the right atrial A-wave. This suggests that the pulmonary valve opens following atrial systole, and then the blood flows into the pulmonary artery in the presystolic phase in patients with constrictive pericarditis. In 2 cases, pulmonary valve echograms were recorded. The a-dip in these 2 cases was as deep as 8.4 mm and 9.6 mm (1.8-4.1 mm with a mean of 2.80 mm for normal men), and its duration was prolonged to 0.17 sec and 0.18 sec (0.10-0.13 sec with a mean of 0.115 sec for normal men). In conclusion, such a deep and prolonged a-dip on pulmonary valve echogram is presumably a sign of presystolic pulmonary valve opening.", "contents": "Presystolic pulmonary valve opening in constrictive pericarditis. Right heart pressure tracings were studied in 5 cases with constrictive pericarditis. The pressure of the A-wave in the right atrium of these cases was higher than the pulmonary arterial diastolic pressure. In addition, a presystolic wave appeared in the pulmonary arterial and the right ventricular pressure tracings, in coincidence with atrial systole. The pressure of this presystolic wave was similar to that of the right atrial A-wave. This suggests that the pulmonary valve opens following atrial systole, and then the blood flows into the pulmonary artery in the presystolic phase in patients with constrictive pericarditis. In 2 cases, pulmonary valve echograms were recorded. The a-dip in these 2 cases was as deep as 8.4 mm and 9.6 mm (1.8-4.1 mm with a mean of 2.80 mm for normal men), and its duration was prolonged to 0.17 sec and 0.18 sec (0.10-0.13 sec with a mean of 0.115 sec for normal men). In conclusion, such a deep and prolonged a-dip on pulmonary valve echogram is presumably a sign of presystolic pulmonary valve opening."}
{"id": "PMID:470142", "title": "Non-invasive determination of left ventricular diastolic compliance.", "content": "The log-log relationship between the instantaneous height of A wave obtained from apexcardiogram and the diameter of minor axis of left ventricle obtained by echocardiography has been calculated. The log-log slope was considered to be an indicator of ventricular stiffness. The slope was significantly higher in different subgroups of cardiac patients compared with normal subjects. This points to its possible value in the study of cardiac function.", "contents": "Non-invasive determination of left ventricular diastolic compliance. The log-log relationship between the instantaneous height of A wave obtained from apexcardiogram and the diameter of minor axis of left ventricle obtained by echocardiography has been calculated. The log-log slope was considered to be an indicator of ventricular stiffness. The slope was significantly higher in different subgroups of cardiac patients compared with normal subjects. This points to its possible value in the study of cardiac function."}
{"id": "PMID:470143", "title": "Glycyl-prolyl-p-nitroanilidase (GPNAase) activity in cardiovascular diseases.", "content": "We have studied serum glycyl-prolyl-p-nitroanilidase (GPNAase) activity and its distribution in various parts of the body in patients with acute myocardial infarction, chronic ischemic heart disease, valvular heart disease with or without congestive heart failure and essential hypertension. Serum GPNAase activity in patients with acute myocardial infarction was significantly lower as long as 12 days after the onset as compared with normal controls. The serum enzyme activity in patients with congestive heart failure was also significantly lower than that of controls and there was a tendency of its gradual decrease with the progress of the disease. There was no significant difference between the activity in control group and that in age-matched patients with essential hypertension. There was no significant change of the activity in patients with chronic ischemic heart disease. The GPNAase activities in sera obtained from various parts of the body by cardiac catheterization were essentially similar.", "contents": "Glycyl-prolyl-p-nitroanilidase (GPNAase) activity in cardiovascular diseases. We have studied serum glycyl-prolyl-p-nitroanilidase (GPNAase) activity and its distribution in various parts of the body in patients with acute myocardial infarction, chronic ischemic heart disease, valvular heart disease with or without congestive heart failure and essential hypertension. Serum GPNAase activity in patients with acute myocardial infarction was significantly lower as long as 12 days after the onset as compared with normal controls. The serum enzyme activity in patients with congestive heart failure was also significantly lower than that of controls and there was a tendency of its gradual decrease with the progress of the disease. There was no significant difference between the activity in control group and that in age-matched patients with essential hypertension. There was no significant change of the activity in patients with chronic ischemic heart disease. The GPNAase activities in sera obtained from various parts of the body by cardiac catheterization were essentially similar."}
{"id": "PMID:470144", "title": "Changes of ventricular monophasic action potential duration by stellate ganglion stimulation in dogs.", "content": "Left or right stellate ganglion stimulation resulted in changes of ST segments and T waves of electrocardiogram. The present experiments were performed in an attempt to elucidate alpha-and beta-adrenergic actions, and calcium action on the monophasic action potential duration (APD), that is, the repolarizing phase of myocardium. During experiments in open-chest dogs, right ventricular pacing was performed for avoiding the acceleration of heart rate by stellate ganglion stimulation. Administration of phentolamine, propranolol or D600 prolonged APD, while hydrazine shortened it. However, stellate ganglion stimulation caused a prolongation of APD after infusion of propranolol or hydrazine, and a shortening of APD after infusion of phentolamine, D600 or hydrazine. These results indicate that alpha-and beta-adrenergic actions as well as calcium ions can be related to APD in the ventricular myocardium of dogs; apparently, alpha-adrenergic action prolongs APD and beta-adrenergic action shortens it.", "contents": "Changes of ventricular monophasic action potential duration by stellate ganglion stimulation in dogs. Left or right stellate ganglion stimulation resulted in changes of ST segments and T waves of electrocardiogram. The present experiments were performed in an attempt to elucidate alpha-and beta-adrenergic actions, and calcium action on the monophasic action potential duration (APD), that is, the repolarizing phase of myocardium. During experiments in open-chest dogs, right ventricular pacing was performed for avoiding the acceleration of heart rate by stellate ganglion stimulation. Administration of phentolamine, propranolol or D600 prolonged APD, while hydrazine shortened it. However, stellate ganglion stimulation caused a prolongation of APD after infusion of propranolol or hydrazine, and a shortening of APD after infusion of phentolamine, D600 or hydrazine. These results indicate that alpha-and beta-adrenergic actions as well as calcium ions can be related to APD in the ventricular myocardium of dogs; apparently, alpha-adrenergic action prolongs APD and beta-adrenergic action shortens it."}
{"id": "PMID:470145", "title": "Coronary collateral circulation as an important factor to modify the ischemic injury of the myocardium in coronary ligated dogs.", "content": "Effect of coronary ligation on epicardial and intramyocardial ST-segment voltage was investigated in 121 dogs. After ligation of the anterior descending coronary artery, marked  ST-elevation, more than 2 mV, was detected in 102 dogs (Group-I) in epicardial lead of the area which was nourished by the ligated coronary artery. In 7 dogs (Group-II), coronary ligation produced negligible changes in ST-segment voltage in epicardial lead, while significant ST-elevation was observed in the leads of middle and inner layers of the myocardium. Systemic hemo-dynamic parameters showed no statistically significant difference between the 2 groups. Coronary collateral indices, however, revealed the significant difference between them. Systolic peripheral coronary pressure was 29.3 +/- 3.1 mmHg in Group-I and 59.2 +/- 10.6 mmHg in Group-II (P less than 0.05). Retrograde flow was also greater in Group-II (14.8 +/- 4.4 ml/min) than in Group-I (1.3 +/- 0.5 ml/min) (p less than 0.01). Diversion of retrograde flow produced a marked ST-elevation in Group-II. Restoration of retrograde flow to the myocardium immediately decreased the ST-segment voltage. These results suggest that spontaneously developed collaterals have an important function for the prevention of the occurrence of ischemic injury after coronary ligation in dogs.", "contents": "Coronary collateral circulation as an important factor to modify the ischemic injury of the myocardium in coronary ligated dogs. Effect of coronary ligation on epicardial and intramyocardial ST-segment voltage was investigated in 121 dogs. After ligation of the anterior descending coronary artery, marked  ST-elevation, more than 2 mV, was detected in 102 dogs (Group-I) in epicardial lead of the area which was nourished by the ligated coronary artery. In 7 dogs (Group-II), coronary ligation produced negligible changes in ST-segment voltage in epicardial lead, while significant ST-elevation was observed in the leads of middle and inner layers of the myocardium. Systemic hemo-dynamic parameters showed no statistically significant difference between the 2 groups. Coronary collateral indices, however, revealed the significant difference between them. Systolic peripheral coronary pressure was 29.3 +/- 3.1 mmHg in Group-I and 59.2 +/- 10.6 mmHg in Group-II (P less than 0.05). Retrograde flow was also greater in Group-II (14.8 +/- 4.4 ml/min) than in Group-I (1.3 +/- 0.5 ml/min) (p less than 0.01). Diversion of retrograde flow produced a marked ST-elevation in Group-II. Restoration of retrograde flow to the myocardium immediately decreased the ST-segment voltage. These results suggest that spontaneously developed collaterals have an important function for the prevention of the occurrence of ischemic injury after coronary ligation in dogs."}
{"id": "PMID:470147", "title": "Inhomogeneous spread of excitation in canine Purkinje fibers.", "content": "Spread of excitation in isolated canine bundle branches was examined by microelectrode technique in order to investigate the possibility of dissociated impulse transmission in normal Purkinje fibers. In all experiments, local excitation of the preparation evoked either by fine bipolar extracellular electrodes or an intracellular microelectrode proceeded much faster along the longitudinal axis of the fibers than along the transverse axis. As a result, the spread of excitation in the vicinity of stimulating site showed significantly inhomogeneous character. The inhomogeneity of excitation spread became more manifest with more eccentric location of the stimulating site in a given preparation. Larger preparation showed greater degree of inhomogeneity. Nevertheless, the inhomogeneous spread of excitation alone appeared unlikely to provide multiply pathways which were functionally dissociated with each other. When premature stimulation was applied, however, different conductivity among fibers within a single false tendon was oberved, suggesting that each conducting element became more independent.", "contents": "Inhomogeneous spread of excitation in canine Purkinje fibers. Spread of excitation in isolated canine bundle branches was examined by microelectrode technique in order to investigate the possibility of dissociated impulse transmission in normal Purkinje fibers. In all experiments, local excitation of the preparation evoked either by fine bipolar extracellular electrodes or an intracellular microelectrode proceeded much faster along the longitudinal axis of the fibers than along the transverse axis. As a result, the spread of excitation in the vicinity of stimulating site showed significantly inhomogeneous character. The inhomogeneity of excitation spread became more manifest with more eccentric location of the stimulating site in a given preparation. Larger preparation showed greater degree of inhomogeneity. Nevertheless, the inhomogeneous spread of excitation alone appeared unlikely to provide multiply pathways which were functionally dissociated with each other. When premature stimulation was applied, however, different conductivity among fibers within a single false tendon was oberved, suggesting that each conducting element became more independent."}
{"id": "PMID:470149", "title": "Electrophysiologic effects of pindolol on atrioventricular conduction in canine heart.", "content": "The effects of intravenous pindolol on the electrophysiologic properties of the atrioventricular conduction system was studied in intact dog, using His bundle electrogram and the extrastimulus method. Pindolol was administered intravenously in a dose range of 4 to 40 micrograms/Kg. The latter dosage of pindolol is above those used clinically. Significant effects of intravenous pindolol were observed on sinus cycle length, the A-V nodal conduction time, the ERP of the atrium, the ERP and FRP of the A-V node, and the ERP of the ventricle. Sinus cycle length was prolonged during sinus rhythm. Intraatrial conduction time was not altered by pindolol, while the ERP of the atrium was slightly increased. The A-H interval was generally prolonged by pindolol without Wenckebach type A-V block, but the H-V interval was unchanged. Both ERP and FRP of the A-V node was prolonged. The ERP and RRP of the His-Purkinje system were not statistically evaluated, because no block within the His-Purkinje system were not statistically evaluated, because no block with the His-Purkinje system or prolongation of H-V interval was produced and only a few QRS complexes by extrastimulus showed aberrant configration in the intact canine heart. In addition, pindolol prolonged the ERP of the ventricle.", "contents": "Electrophysiologic effects of pindolol on atrioventricular conduction in canine heart. The effects of intravenous pindolol on the electrophysiologic properties of the atrioventricular conduction system was studied in intact dog, using His bundle electrogram and the extrastimulus method. Pindolol was administered intravenously in a dose range of 4 to 40 micrograms/Kg. The latter dosage of pindolol is above those used clinically. Significant effects of intravenous pindolol were observed on sinus cycle length, the A-V nodal conduction time, the ERP of the atrium, the ERP and FRP of the A-V node, and the ERP of the ventricle. Sinus cycle length was prolonged during sinus rhythm. Intraatrial conduction time was not altered by pindolol, while the ERP of the atrium was slightly increased. The A-H interval was generally prolonged by pindolol without Wenckebach type A-V block, but the H-V interval was unchanged. Both ERP and FRP of the A-V node was prolonged. The ERP and RRP of the His-Purkinje system were not statistically evaluated, because no block within the His-Purkinje system were not statistically evaluated, because no block with the His-Purkinje system or prolongation of H-V interval was produced and only a few QRS complexes by extrastimulus showed aberrant configration in the intact canine heart. In addition, pindolol prolonged the ERP of the ventricle."}
{"id": "PMID:470151", "title": "Electrophysiological studies in two patients with dystrophia myotonica and atrioventricular conduction block.", "content": "Two patients with dystrophia myotonica showed high-grade atrio-ventricular block. Both underwent electrophysiological studies which revealed sinus and A-V nodal disease with normal intraventricular conduction in 1 case and His-Purkinje conduction disease in the other. Dystrophia myotonica may, therfore, involve all parts of the cardiac conduction system and may affect the generation of cardiac impulses. Pacemaker implantation may be necessary especially if drugs such as procainamide, which in addition to controlling myotonic symptoms may aggravate conduction disorders, are to be used.", "contents": "Electrophysiological studies in two patients with dystrophia myotonica and atrioventricular conduction block. Two patients with dystrophia myotonica showed high-grade atrio-ventricular block. Both underwent electrophysiological studies which revealed sinus and A-V nodal disease with normal intraventricular conduction in 1 case and His-Purkinje conduction disease in the other. Dystrophia myotonica may, therfore, involve all parts of the cardiac conduction system and may affect the generation of cardiac impulses. Pacemaker implantation may be necessary especially if drugs such as procainamide, which in addition to controlling myotonic symptoms may aggravate conduction disorders, are to be used."}
{"id": "PMID:470184", "title": "Immunochemical studies of lipids. V. Effect of modified hydrophobic moiety on immunogenicity and immunologic reactivity of Forssman glycolipid.", "content": "The role of hydrophobic moiety in determining the immunologic properties of glycolipid haptens was studied with the derivatives of Forssman glycolipid modified by ozonolytic cleavage of double bonds in hydrocarbon chains. The immunogenicity was not affected by this modification; comparable antibody responses were elicited in rabbits immunized with intact or modified Forssman glycolipid, regardless of its different hydrophobicity, in complete Freunds adjuvant. Modified hydrophobic portions did not affect the class and specificity of antibodies produced. On the other hand, immunologic reactivity was strongly influenced by hydrophobic moiety; the ability to form immune precipitates with antibodies decreased as the hydrophobicity of glycolipid decreased. The reactivity in complement fixation and hemolysis inhibition tests also showed parallel decreases, though the addition of auxiliary lipids greatly reduced these differences in reactivity.  The results indicate the different influences of hydrophobic moiety of glycolipids on their immunogenicity and immunologic reactivity in vitro.", "contents": "Immunochemical studies of lipids. V. Effect of modified hydrophobic moiety on immunogenicity and immunologic reactivity of Forssman glycolipid. The role of hydrophobic moiety in determining the immunologic properties of glycolipid haptens was studied with the derivatives of Forssman glycolipid modified by ozonolytic cleavage of double bonds in hydrocarbon chains. The immunogenicity was not affected by this modification; comparable antibody responses were elicited in rabbits immunized with intact or modified Forssman glycolipid, regardless of its different hydrophobicity, in complete Freunds adjuvant. Modified hydrophobic portions did not affect the class and specificity of antibodies produced. On the other hand, immunologic reactivity was strongly influenced by hydrophobic moiety; the ability to form immune precipitates with antibodies decreased as the hydrophobicity of glycolipid decreased. The reactivity in complement fixation and hemolysis inhibition tests also showed parallel decreases, though the addition of auxiliary lipids greatly reduced these differences in reactivity.  The results indicate the different influences of hydrophobic moiety of glycolipids on their immunogenicity and immunologic reactivity in vitro."}
{"id": "PMID:470185", "title": "Experimental Pseudomonas infection in mice: effect of single cyclophosphamide administration on Pseudomonas infection.", "content": "The kinetic effect of cyclophosphamide (CY) was investigated using the immune response to sheep red blood cells (SRBC), activation of the mononuclear macrophage system and altered susceptibity to pseudomonas infection. The level of delayed type hypersensitivity (DTH) was enhanced with suppressed antibody formation, when antigenic stimulation was given about 3 days after CY administration. In contrast, antibody formation increased markedly when challenged with antigen about 10 days after CY administration. Activity of macrophage system as measured by rate of carbon clearance and spreading of peritoneal macrophage was decreased within 6 days after CY, therefore increased to a peak at the 13th day of CY administration. CY increased a susceptibility to pseudomonas infection at the early time of its administration such as the 3rd day, whereas more increased resistance was observed at the later time such as the 13th day. These results indicated that B-lymphocyte depletion included by administration of sublethal dose of CY (200mg/kg) was followed by vigorous hyperplasia of B-lymphocyte.", "contents": "Experimental Pseudomonas infection in mice: effect of single cyclophosphamide administration on Pseudomonas infection. The kinetic effect of cyclophosphamide (CY) was investigated using the immune response to sheep red blood cells (SRBC), activation of the mononuclear macrophage system and altered susceptibity to pseudomonas infection. The level of delayed type hypersensitivity (DTH) was enhanced with suppressed antibody formation, when antigenic stimulation was given about 3 days after CY administration. In contrast, antibody formation increased markedly when challenged with antigen about 10 days after CY administration. Activity of macrophage system as measured by rate of carbon clearance and spreading of peritoneal macrophage was decreased within 6 days after CY, therefore increased to a peak at the 13th day of CY administration. CY increased a susceptibility to pseudomonas infection at the early time of its administration such as the 3rd day, whereas more increased resistance was observed at the later time such as the 13th day. These results indicated that B-lymphocyte depletion included by administration of sublethal dose of CY (200mg/kg) was followed by vigorous hyperplasia of B-lymphocyte."}
{"id": "PMID:470186", "title": "Studies on the physicochemical properties of house dust mite (Dermatophagoides pteronyssinus) allergens involved in reaginic reaction.", "content": "The physicochemical nature of the allergenic component of Dermatophagoides pteronyssinus extract was studied by gel filtration, ion exchange chromatography, isoelectrofocusing, and polyacrylamide gel electrophoresis. Fractions were assayed for allergenic activity by the direct radioallergosorbent test (RAST) (dilution method), RAST inhibition (50% inhibition method) and human skin testing. Fractions were further studied by preparative polyacrylamide gel electrophoresis and the band showing a flow rate value of 0.35 was the most potent in allergenic activity. Fractionation by isoelectrofocusing yielded three factions with allergenic activity of pI = 4.8, PI = 7.4 and pI = 10.0 Sephadex G-100 gel filtration revealed that allergenic activity was present in fractions with molecular weight ranging from 17,000-45,000 and discrete ion exchange chromatography evidenced three fractions with very high allergenic activity. In the present study we propose that the allergenic material of D.P is composed of 3 physicochemically different substances whose molecular weight lie in the 17,000 to 45,000 range.", "contents": "Studies on the physicochemical properties of house dust mite (Dermatophagoides pteronyssinus) allergens involved in reaginic reaction. The physicochemical nature of the allergenic component of Dermatophagoides pteronyssinus extract was studied by gel filtration, ion exchange chromatography, isoelectrofocusing, and polyacrylamide gel electrophoresis. Fractions were assayed for allergenic activity by the direct radioallergosorbent test (RAST) (dilution method), RAST inhibition (50% inhibition method) and human skin testing. Fractions were further studied by preparative polyacrylamide gel electrophoresis and the band showing a flow rate value of 0.35 was the most potent in allergenic activity. Fractionation by isoelectrofocusing yielded three factions with allergenic activity of pI = 4.8, PI = 7.4 and pI = 10.0 Sephadex G-100 gel filtration revealed that allergenic activity was present in fractions with molecular weight ranging from 17,000-45,000 and discrete ion exchange chromatography evidenced three fractions with very high allergenic activity. In the present study we propose that the allergenic material of D.P is composed of 3 physicochemically different substances whose molecular weight lie in the 17,000 to 45,000 range."}
{"id": "PMID:470188", "title": "Establishment and characterization of a human neuroblastoma cell line in tissue culture.", "content": "A new continuous cell line, GOTO, has been established in tissue culture from a human neuroblastoma arising from the adrenal and maintained for 5 years. The cells are small and fibroblast-like, and grow in dense layer. Population doubling time was approximately 48 hr. The modal chromosome number was near-diploid. Neither marker  chromosomes nor double-minute chromosomes were abserved. Inoculum of 10(7) cells produced tumors in ATS-treated hamsters and athymic nude mice, and the histological appearance of tumors was consistent with undifferentiated neuroblastoma. Neural features of the cells were also confirmed by ultrastructural examination. Biochemical analysis showed this cell line to be cholinergic. Morphological differentiation leading to neurite formation was induced by cultivation in serum-free medium.", "contents": "Establishment and characterization of a human neuroblastoma cell line in tissue culture. A new continuous cell line, GOTO, has been established in tissue culture from a human neuroblastoma arising from the adrenal and maintained for 5 years. The cells are small and fibroblast-like, and grow in dense layer. Population doubling time was approximately 48 hr. The modal chromosome number was near-diploid. Neither marker  chromosomes nor double-minute chromosomes were abserved. Inoculum of 10(7) cells produced tumors in ATS-treated hamsters and athymic nude mice, and the histological appearance of tumors was consistent with undifferentiated neuroblastoma. Neural features of the cells were also confirmed by ultrastructural examination. Biochemical analysis showed this cell line to be cholinergic. Morphological differentiation leading to neurite formation was induced by cultivation in serum-free medium."}
{"id": "PMID:470209", "title": "[An investigation of the environment in a certain vanadium refinery (author's transl)].", "content": "An investigation of the environment of the working place was made as part of the investigation of damages to health that had broken out in a certain vanadium refinery; and the following conclusions were obtained: When the atmospheric dust volume and V2O5 concentration by unit working step were measured with a high volume air sampler, V2O5 exceeded the threshold limit value given by ACGIH in the P.O. flake production step, while the measurement of V2O5 concentration during one-shift working hours with a low volume air sampler gave 64.3 micrograms/m3 even in the grinding step where the maximum concentration was found, and values under 10 micrograms/m3 in all the other steps. Such concentrations may be considered to have been derived from the fact that P.O. casting in accomplished in a short time, and that the fume and dust that are transiently produced at high concentrations are dispersed outdoors by spontaneous ventilation. Damages to health such as a high prevalence of green tongue appeared to have resulted from failure in exercising elementary sanitary care such as imperfect facilities for safety and sanitation, and failure to thoroughly enforce wearing of masks.", "contents": "[An investigation of the environment in a certain vanadium refinery (author's transl)]. An investigation of the environment of the working place was made as part of the investigation of damages to health that had broken out in a certain vanadium refinery; and the following conclusions were obtained: When the atmospheric dust volume and V2O5 concentration by unit working step were measured with a high volume air sampler, V2O5 exceeded the threshold limit value given by ACGIH in the P.O. flake production step, while the measurement of V2O5 concentration during one-shift working hours with a low volume air sampler gave 64.3 micrograms/m3 even in the grinding step where the maximum concentration was found, and values under 10 micrograms/m3 in all the other steps. Such concentrations may be considered to have been derived from the fact that P.O. casting in accomplished in a short time, and that the fume and dust that are transiently produced at high concentrations are dispersed outdoors by spontaneous ventilation. Damages to health such as a high prevalence of green tongue appeared to have resulted from failure in exercising elementary sanitary care such as imperfect facilities for safety and sanitation, and failure to thoroughly enforce wearing of masks."}
{"id": "PMID:470210", "title": "[Results of the special physical examination of workers in a vanadium plant (author's transl)].", "content": "Special physical examinations were made in order to find out the actual status of damages to health that had broken out in a factory collecting V2O5 from iron sand; and the following results were found: 1. Pharyngitis and bronchitis were found in 25% of the workers exposed to vanadium, but neither pneumonia nor hepatitis was observed. 2. Among the subjective and objective symptoms, respiratory irritation and discoloration of the tongue were frequent. 3. Black spot-like pigmentations gathering in a zonal form 1-2 mm wide in the transitional part and oral mucosa of the upper lip were found. Prevalence rate of this sign was 14.3% in the workers exposed to vanadium. 4. The mean valus of total serum protein and the serum cholesterol in the exposed workers were lower than those in the controls. The difference in the values between both groups is statistically significant. 5. Both the mean values of vanadium concentrations in vurine and its creatinine ratios in the exposed workers were twice to three times those in the controls; however, these parameters decreased to about one third in two months by improving the health and environmental control-measures. 6. Draft items to be checked in special physical examinations of workers handling vanadium have been proposed.", "contents": "[Results of the special physical examination of workers in a vanadium plant (author's transl)]. Special physical examinations were made in order to find out the actual status of damages to health that had broken out in a factory collecting V2O5 from iron sand; and the following results were found: 1. Pharyngitis and bronchitis were found in 25% of the workers exposed to vanadium, but neither pneumonia nor hepatitis was observed. 2. Among the subjective and objective symptoms, respiratory irritation and discoloration of the tongue were frequent. 3. Black spot-like pigmentations gathering in a zonal form 1-2 mm wide in the transitional part and oral mucosa of the upper lip were found. Prevalence rate of this sign was 14.3% in the workers exposed to vanadium. 4. The mean valus of total serum protein and the serum cholesterol in the exposed workers were lower than those in the controls. The difference in the values between both groups is statistically significant. 5. Both the mean values of vanadium concentrations in vurine and its creatinine ratios in the exposed workers were twice to three times those in the controls; however, these parameters decreased to about one third in two months by improving the health and environmental control-measures. 6. Draft items to be checked in special physical examinations of workers handling vanadium have been proposed."}
{"id": "PMID:470211", "title": "[Testicular atrophy of mice induced by ethylene glycol mono alkyl ethers (author's transl)].", "content": "Toxicities of ethylene glycol (EG) and 6 ethylene glycol mono alkyl ethers administered orally were studied. Mice were given various doses (62.5, 125, 250, 500, 1,000, 2,000 and 4,000 mg/kg body weight) of the compounds daily for 5 days/week, for 5 weeks. High doses of ethylene glycol mono methyl ether (EGM), ethylene glycol mono methyl ether acetate (EGMA), ethylene glycol mono ethyl ether (EGE) and ethylene glycol mono ethyl acetate (EGEA) produced marked testicular atrophy and leucopenia. Dose-responce relation was found in these effects. EGM was more effective than EGE, while ethylene glycol mono butyl ether and ethylene glycol mono phenyl ether had but slight effect and EG had no detectable action on testis and leucocytes. Toxic doses being expressed as mg/kg body weight, esterification seemed to weaken the atrophic action of EGM and EGE, but when expressed as mol/kg, significant difference was found neither between EGM and EGMA nor between EGE and EGEA. The mechanism of testicular atrophy induced by low ethylene glycol mono alkyl ethers is likely to be an inhibitory action on cell division.", "contents": "[Testicular atrophy of mice induced by ethylene glycol mono alkyl ethers (author's transl)]. Toxicities of ethylene glycol (EG) and 6 ethylene glycol mono alkyl ethers administered orally were studied. Mice were given various doses (62.5, 125, 250, 500, 1,000, 2,000 and 4,000 mg/kg body weight) of the compounds daily for 5 days/week, for 5 weeks. High doses of ethylene glycol mono methyl ether (EGM), ethylene glycol mono methyl ether acetate (EGMA), ethylene glycol mono ethyl ether (EGE) and ethylene glycol mono ethyl acetate (EGEA) produced marked testicular atrophy and leucopenia. Dose-responce relation was found in these effects. EGM was more effective than EGE, while ethylene glycol mono butyl ether and ethylene glycol mono phenyl ether had but slight effect and EG had no detectable action on testis and leucocytes. Toxic doses being expressed as mg/kg body weight, esterification seemed to weaken the atrophic action of EGM and EGE, but when expressed as mol/kg, significant difference was found neither between EGM and EGMA nor between EGE and EGEA. The mechanism of testicular atrophy induced by low ethylene glycol mono alkyl ethers is likely to be an inhibitory action on cell division."}
{"id": "PMID:470212", "title": "[Combustion gas toxicity of textiles (author's transl)].", "content": "Textiles are used so widely as interior materials of many buildings, houses, and transportation facilities, that the combustion gas has a chance of intoxication of a fire accident. Therefore this investigation was undertaken to clarify the toxicity of the gas and to obtain an index for safety standards in such cases. The combustion gases of cotton, polypropylene, acrylic, flame retardant (F.R.) cotton and F.R. acrylic fabrics were analyzed by a gas chromatograph and a GC-Mass spectorograph, and also tested with experimental animals to determine the lethal factors including blood gas analyzing technique. The gas exposuring test based on gas analysis data showed that the toxicity of these combustion products increased in the following orders: cotton, polypropylene, F.R. cotton, F.R. acrylic and acrylic. Comparative indices of the toxicity showed that acrylic increased 7.26-fold as compared with that of cotton. It was recognized that the lethal factors in the mice was due to carbon monoxide and/or hydrogen cyanide during the exposure one hour, and that sequelae due to acrolein during the seven day observation period after the exposure. Flame retardant treatment increased twice the toxicity of cotton. On the contrary, that of acrylic was reduced by 0.8 time compared with the original fabric, so that the treatment could be recommended only in case of acrylic from the safety standpoint. From the above results, it can be stressed that vigorous smoke exhaust procedure should be executed in the case of fire accidents for saving human lives.", "contents": "[Combustion gas toxicity of textiles (author's transl)]. Textiles are used so widely as interior materials of many buildings, houses, and transportation facilities, that the combustion gas has a chance of intoxication of a fire accident. Therefore this investigation was undertaken to clarify the toxicity of the gas and to obtain an index for safety standards in such cases. The combustion gases of cotton, polypropylene, acrylic, flame retardant (F.R.) cotton and F.R. acrylic fabrics were analyzed by a gas chromatograph and a GC-Mass spectorograph, and also tested with experimental animals to determine the lethal factors including blood gas analyzing technique. The gas exposuring test based on gas analysis data showed that the toxicity of these combustion products increased in the following orders: cotton, polypropylene, F.R. cotton, F.R. acrylic and acrylic. Comparative indices of the toxicity showed that acrylic increased 7.26-fold as compared with that of cotton. It was recognized that the lethal factors in the mice was due to carbon monoxide and/or hydrogen cyanide during the exposure one hour, and that sequelae due to acrolein during the seven day observation period after the exposure. Flame retardant treatment increased twice the toxicity of cotton. On the contrary, that of acrylic was reduced by 0.8 time compared with the original fabric, so that the treatment could be recommended only in case of acrylic from the safety standpoint. From the above results, it can be stressed that vigorous smoke exhaust procedure should be executed in the case of fire accidents for saving human lives."}
{"id": "PMID:470214", "title": "[ALAD activity of red cells of different ages. (1st report) (author's transl)].", "content": "Rabbit erythrocytes were fractionated into populations of young cells (top layer) and old ones (bottom layer) by centrifugation, based on the fact that the specific gravity of young erythrocytes was lower than that of old ones. Then, reticurocyte count and osmotic resistance of red cells were compared between both populations. As a result, it has been obtained that many reticurocytes were found in the top layer, while none of those was found in the bottom layer. Furthermore, cells of the top layer were more resistant to hemolysis in the hypotonic media than those of the bottom layer. These findings suggested that the cell population of the top layer was young and that of the bottom layer was old. In comparison between both populations, the mean value of ALAD activity of young cells was 2.6 times higher than that of old ones. The rate of decrease of ALAD activity after intravenous administration of lead was similar in both populations while the amount of decrease of ALAD activity in the young cells was 2 to 3 times higher than that of the old ones.", "contents": "[ALAD activity of red cells of different ages. (1st report) (author's transl)]. Rabbit erythrocytes were fractionated into populations of young cells (top layer) and old ones (bottom layer) by centrifugation, based on the fact that the specific gravity of young erythrocytes was lower than that of old ones. Then, reticurocyte count and osmotic resistance of red cells were compared between both populations. As a result, it has been obtained that many reticurocytes were found in the top layer, while none of those was found in the bottom layer. Furthermore, cells of the top layer were more resistant to hemolysis in the hypotonic media than those of the bottom layer. These findings suggested that the cell population of the top layer was young and that of the bottom layer was old. In comparison between both populations, the mean value of ALAD activity of young cells was 2.6 times higher than that of old ones. The rate of decrease of ALAD activity after intravenous administration of lead was similar in both populations while the amount of decrease of ALAD activity in the young cells was 2 to 3 times higher than that of the old ones."}
{"id": "PMID:470216", "title": "[Evaluation of toluene toxicity using Sidman avoidance behavior by lever pressing in rats (author's transl)].", "content": "Effects of single exposure to toluene on the operant behavior in rats were studied. The animals, in which the behavioral base line had been established, were exposed to 0, 1,000 and 3,000 ppm of toluene vapor for four hours, and the effects on the behavioral pattern of lever pressing were investigated for one hour after the end of exposure using Sidman avoidance test. 1) Exposure to 0 or 1,000 ppm of toluene did not affect the established operant behavior of the rats. 2) Exposure to 3,000 ppm produced increased responses, shortening of the inter-response-time, and no change in shock counts at the early stage of Sidman avoidance test when compared with those before exposure. 3) The behavioral change indicated here appears to be caused by a temporary breakdown of the established timing of lever pressing. 4) The effect of toluene on the operant behavior was strongest immediately after the aviodance test begun and the behavior recovered in about an hour. From the results of our experiment it can be said that the experimental methods used in this study is useful to evaluate the toxicity of toluene to the central nervous system of the animals.", "contents": "[Evaluation of toluene toxicity using Sidman avoidance behavior by lever pressing in rats (author's transl)]. Effects of single exposure to toluene on the operant behavior in rats were studied. The animals, in which the behavioral base line had been established, were exposed to 0, 1,000 and 3,000 ppm of toluene vapor for four hours, and the effects on the behavioral pattern of lever pressing were investigated for one hour after the end of exposure using Sidman avoidance test. 1) Exposure to 0 or 1,000 ppm of toluene did not affect the established operant behavior of the rats. 2) Exposure to 3,000 ppm produced increased responses, shortening of the inter-response-time, and no change in shock counts at the early stage of Sidman avoidance test when compared with those before exposure. 3) The behavioral change indicated here appears to be caused by a temporary breakdown of the established timing of lever pressing. 4) The effect of toluene on the operant behavior was strongest immediately after the aviodance test begun and the behavior recovered in about an hour. From the results of our experiment it can be said that the experimental methods used in this study is useful to evaluate the toxicity of toluene to the central nervous system of the animals."}
{"id": "PMID:470220", "title": "Immunotherapeutic trials of murine and guinea-pig solid tumors by oral administration of BCG.", "content": "Efficacy of oral administration of BCG on the growth of various tumors in mice and guinea pigs was studied. The growth-inhibitory effect varied depending on the tumor systems and the experimental conditions. Weekly oral administrations with 5-mg doses of BCG to mice or 80-mg doses of BCG to guinea pigs were ineffective on syngeneic mouse melanoma B16 or syngeneic guinea pig hepatocarcinoma line-10 but effective on syngeneic mouse carcinoma IMC and syngeneic guinea-pig fibrosarcoma H9A. Oral BCG seemed effective also on allogeneic mouse carcinoma Ehrlich, developed with a relatively small size of tumor cell inoculum, and on guinea-pig syngeneic liposarcoma H10. On Ehrlich tumors, oral BCG given once a week seemed to have better effects than did oral BCG given twice a week or subcutaneously once or repeatedly; heat-killed BCG given orally showed no effect. However, it seems premature to draw a definite conclusion on the efficacy of oral BCG on Ehrlich and H10 tumors, because some of these tumors regressed spontaneously even in nontreated control animals. The host responses to oral BCG were studied with the following results. Weekly oral administration with 80-mg doses of BCG to guinea pigs elicited positive skin reactions to 25 TU PPD in about 65 days after the first BCG, while a single sc injection of 8 mg of BCG did so within 10 days. Orally administered BCG organisms were recovered largely from Peyer's patches, a little from the mesenteric lymph nodes, and very little from the liver and the spleen. The BCG distributive pattern was in reverse order when BCG was given subcutaneously. Histologic examinations of Peyer's patches indicated enlargement of germinal centers, in which primitive reticular cells proliferated prominently and the macrophages with tingible bodies scattered frequently.", "contents": "Immunotherapeutic trials of murine and guinea-pig solid tumors by oral administration of BCG. Efficacy of oral administration of BCG on the growth of various tumors in mice and guinea pigs was studied. The growth-inhibitory effect varied depending on the tumor systems and the experimental conditions. Weekly oral administrations with 5-mg doses of BCG to mice or 80-mg doses of BCG to guinea pigs were ineffective on syngeneic mouse melanoma B16 or syngeneic guinea pig hepatocarcinoma line-10 but effective on syngeneic mouse carcinoma IMC and syngeneic guinea-pig fibrosarcoma H9A. Oral BCG seemed effective also on allogeneic mouse carcinoma Ehrlich, developed with a relatively small size of tumor cell inoculum, and on guinea-pig syngeneic liposarcoma H10. On Ehrlich tumors, oral BCG given once a week seemed to have better effects than did oral BCG given twice a week or subcutaneously once or repeatedly; heat-killed BCG given orally showed no effect. However, it seems premature to draw a definite conclusion on the efficacy of oral BCG on Ehrlich and H10 tumors, because some of these tumors regressed spontaneously even in nontreated control animals. The host responses to oral BCG were studied with the following results. Weekly oral administration with 80-mg doses of BCG to guinea pigs elicited positive skin reactions to 25 TU PPD in about 65 days after the first BCG, while a single sc injection of 8 mg of BCG did so within 10 days. Orally administered BCG organisms were recovered largely from Peyer's patches, a little from the mesenteric lymph nodes, and very little from the liver and the spleen. The BCG distributive pattern was in reverse order when BCG was given subcutaneously. Histologic examinations of Peyer's patches indicated enlargement of germinal centers, in which primitive reticular cells proliferated prominently and the macrophages with tingible bodies scattered frequently."}
{"id": "PMID:470221", "title": "Application of the parallel line bioassay method to quantitative determination of the HBs antigen in radioimmunoassay.", "content": "Precise and reproducible quantitative determination of hepatitis B surface antigen by applying the parallel line bioassay method was proposed. In this method, the value of any given sample can be expressed in terms of a relative value to a fixed standard or reference preparation. Decrease in antigenic reactivity due to the lowered protein content could be prevented by using 0.02% human albumin in physiological saline solution as the diluent. The use of such stabilizer(s) should be considered in the dilution or storage of samples containing HBs antigen.", "contents": "Application of the parallel line bioassay method to quantitative determination of the HBs antigen in radioimmunoassay. Precise and reproducible quantitative determination of hepatitis B surface antigen by applying the parallel line bioassay method was proposed. In this method, the value of any given sample can be expressed in terms of a relative value to a fixed standard or reference preparation. Decrease in antigenic reactivity due to the lowered protein content could be prevented by using 0.02% human albumin in physiological saline solution as the diluent. The use of such stabilizer(s) should be considered in the dilution or storage of samples containing HBs antigen."}
{"id": "PMID:470249", "title": "Evaluation of gastrectomy combined with the resection of other organs in the treatment of gastric cancer.", "content": "We evaluated the prognostic significance of gastrectomy combined with resection of adjacent organs in 154 cases. Patients who had undergone gastrectomy with or without combined splenectomy showed similar results and cases with combined colectomy showed a poor survival rate. However, relatively favorable results were obtained in gastrectomy cases in which combined pancreas or liver resection was performed due to direct cancer infiltration.", "contents": "Evaluation of gastrectomy combined with the resection of other organs in the treatment of gastric cancer. We evaluated the prognostic significance of gastrectomy combined with resection of adjacent organs in 154 cases. Patients who had undergone gastrectomy with or without combined splenectomy showed similar results and cases with combined colectomy showed a poor survival rate. However, relatively favorable results were obtained in gastrectomy cases in which combined pancreas or liver resection was performed due to direct cancer infiltration."}
{"id": "PMID:470250", "title": "Specific immunotherapy for non-curatively resected gastric cancer.", "content": "The prognosis of patients with advanced gastric cancer treated with non-curative resection is very poor. In order to improve patient prognosis, we have tried specific immunotherapy as an adjunct to surgery since January 1968. Sixty-two patients with gastric cancer underwent non-curative resection during the period of this study. Twenty-eight of these patients were treated with specific immunotherapy after surgery, and 34 patients received no additional therapy. The five year survival rate was 35 per cent in the former group, and zero in the latter. No significant difference in the degree of tumor extension and invasion was noted between the two patient groups. We concluded that specific immunotherapy as an adjunct to non-curative resection for advanced cancer is worthy of further study.", "contents": "Specific immunotherapy for non-curatively resected gastric cancer. The prognosis of patients with advanced gastric cancer treated with non-curative resection is very poor. In order to improve patient prognosis, we have tried specific immunotherapy as an adjunct to surgery since January 1968. Sixty-two patients with gastric cancer underwent non-curative resection during the period of this study. Twenty-eight of these patients were treated with specific immunotherapy after surgery, and 34 patients received no additional therapy. The five year survival rate was 35 per cent in the former group, and zero in the latter. No significant difference in the degree of tumor extension and invasion was noted between the two patient groups. We concluded that specific immunotherapy as an adjunct to non-curative resection for advanced cancer is worthy of further study."}
{"id": "PMID:470251", "title": "Clinical value of immunochemotherapy with OK-432 or PS-K for stomach cancer patients.", "content": "A prospective clinical trial was undertaken in 121 patients with stomach cancer to compare immunochemotherapy with 5-fluorouracil and FT-207 combined with OK-432 or PS-K, immunostimulators, and plain chemotherapy with 5-fluororacil and FT-207. Of the 121 patients who received immunochemotherapy, 67 patients (group A) had undergone curative removal of the tumor. The other 54 patients had undergone noncurative tumor removal or had recurrence after non-curative tumor removal and they were divided into two groups (groups B and C) on the basis of lymphocyte reactivity induced with PHA. Although group A exhibited a significant increase in PHA-induced lymphocyte transformation and a trifling increase in lymphocyte counts, its survival rate within a 36 month period did not differ from that of the peer controls. Group B, composed of 21 patients showing improvement of PHA-induced lymphocyte transformation, significantly prolonged its survival compared to the peer controls. The survival of group C, composed of 33 patients showing a gradual drop in PHA-induced lymphocyte transformation, was not prolonged compared to the peer control patients; and they showed significant decreases in lymphocyte counts. The overall survival of group B and group C was not superior to that of the 48 peer controls.", "contents": "Clinical value of immunochemotherapy with OK-432 or PS-K for stomach cancer patients. A prospective clinical trial was undertaken in 121 patients with stomach cancer to compare immunochemotherapy with 5-fluorouracil and FT-207 combined with OK-432 or PS-K, immunostimulators, and plain chemotherapy with 5-fluororacil and FT-207. Of the 121 patients who received immunochemotherapy, 67 patients (group A) had undergone curative removal of the tumor. The other 54 patients had undergone noncurative tumor removal or had recurrence after non-curative tumor removal and they were divided into two groups (groups B and C) on the basis of lymphocyte reactivity induced with PHA. Although group A exhibited a significant increase in PHA-induced lymphocyte transformation and a trifling increase in lymphocyte counts, its survival rate within a 36 month period did not differ from that of the peer controls. Group B, composed of 21 patients showing improvement of PHA-induced lymphocyte transformation, significantly prolonged its survival compared to the peer controls. The survival of group C, composed of 33 patients showing a gradual drop in PHA-induced lymphocyte transformation, was not prolonged compared to the peer control patients; and they showed significant decreases in lymphocyte counts. The overall survival of group B and group C was not superior to that of the 48 peer controls."}
{"id": "PMID:470252", "title": "Pathological changes of anastomotic false aneurysms.", "content": "Anastomotic parts of polyester vascular prostheses implanted in thoracic aortae of 270 dogs were examined. Anastomotic false aneurysms were seen with 3 cases (1.2%), in which disruption did not occur, however, the grafts which have no aneurysm showed the following features, i.e., suture migration, degenerative changes of the host arterial wall sustaining a suture and dissection between the host arterial wall and a prosthesis. From the results of an analysis of the arrangement pattern of smooth muscle cells in neointima, it was clarified that a suture had been subjected to continuous tensile stress. So that, the host arterial wall sustaining a suture is clinched and pulled toward prosthesis by the suture at every pulsation during implantation. These results indicate that the features mentioned above are phenomena of the developing into anastomotic false aneurysms.", "contents": "Pathological changes of anastomotic false aneurysms. Anastomotic parts of polyester vascular prostheses implanted in thoracic aortae of 270 dogs were examined. Anastomotic false aneurysms were seen with 3 cases (1.2%), in which disruption did not occur, however, the grafts which have no aneurysm showed the following features, i.e., suture migration, degenerative changes of the host arterial wall sustaining a suture and dissection between the host arterial wall and a prosthesis. From the results of an analysis of the arrangement pattern of smooth muscle cells in neointima, it was clarified that a suture had been subjected to continuous tensile stress. So that, the host arterial wall sustaining a suture is clinched and pulled toward prosthesis by the suture at every pulsation during implantation. These results indicate that the features mentioned above are phenomena of the developing into anastomotic false aneurysms."}
{"id": "PMID:470253", "title": "Long-term prognosis for reconstruction of arterial lesions due to arteriosclerosis.", "content": "Out of 769 patients with arterial diseases, reconstructive surgery was performed on 100 limbs of 79 patients for arteriosclerosis. The overall patency rate was 59 per cent over a period of 3 to 8 years. Long-term patency was influenced by the condition of the run-off arteries, the site of the operation, and the method of surgery. No relation was found between patency rate and hypertension, cardiac insufficiency, total serum cholesterol, diabetes mellitus, or age. The survival rate was 62 per cent at 5 years and 48 per cent at 7 years. These rates were significantly poor (p less than 0.001), compared with those in the normal population. Mortality was related to the degree of hypertension, with cardiac and renal failure being responsible for 72 per cent of deaths. Aggressive reconstruction may be indicated in cases with hypertension of stage 2 or below based on the WHO classification. However, especially in patients with associated diabetes mellitus close long-term observation of the cardiovascular system is necessary.", "contents": "Long-term prognosis for reconstruction of arterial lesions due to arteriosclerosis. Out of 769 patients with arterial diseases, reconstructive surgery was performed on 100 limbs of 79 patients for arteriosclerosis. The overall patency rate was 59 per cent over a period of 3 to 8 years. Long-term patency was influenced by the condition of the run-off arteries, the site of the operation, and the method of surgery. No relation was found between patency rate and hypertension, cardiac insufficiency, total serum cholesterol, diabetes mellitus, or age. The survival rate was 62 per cent at 5 years and 48 per cent at 7 years. These rates were significantly poor (p less than 0.001), compared with those in the normal population. Mortality was related to the degree of hypertension, with cardiac and renal failure being responsible for 72 per cent of deaths. Aggressive reconstruction may be indicated in cases with hypertension of stage 2 or below based on the WHO classification. However, especially in patients with associated diabetes mellitus close long-term observation of the cardiovascular system is necessary."}
{"id": "PMID:470254", "title": "A manometric assessment of anorectal pressures and its significance in the diagnosis of Hirschsprung's disease and idiopathic megacolon.", "content": "Anorectal manometric studies were performed on nine patients with Hirschsprung's disease and ten patients with idiopathic megacolon for the purpose of differentiation, comparing with those of the normal subjects. The anorectal reflex was absent in all nine of the patients with Hirschsprung's disease while in all of ten patients with idiopathic megacolon rectal distension produced a relaxation in the anal canal like that of the normal subjects. Manometric studies proved to be a reliable and non-invasive technique for the diagnosis of Hirschsprung's disease and were of particular value as simple screening tests in patients with a clinical suggestion of this disease. The manometric studies of the patients with Hirschsprung's disease or idiopathic megacolon in the present series also demonstrated that the pronounced internal sphincter contraction combined with a total absence of reflex relaxation or an inadequate response of the sphincter to rectal distension might be responsible for the obstructive symptoms in these diseases.", "contents": "A manometric assessment of anorectal pressures and its significance in the diagnosis of Hirschsprung's disease and idiopathic megacolon. Anorectal manometric studies were performed on nine patients with Hirschsprung's disease and ten patients with idiopathic megacolon for the purpose of differentiation, comparing with those of the normal subjects. The anorectal reflex was absent in all nine of the patients with Hirschsprung's disease while in all of ten patients with idiopathic megacolon rectal distension produced a relaxation in the anal canal like that of the normal subjects. Manometric studies proved to be a reliable and non-invasive technique for the diagnosis of Hirschsprung's disease and were of particular value as simple screening tests in patients with a clinical suggestion of this disease. The manometric studies of the patients with Hirschsprung's disease or idiopathic megacolon in the present series also demonstrated that the pronounced internal sphincter contraction combined with a total absence of reflex relaxation or an inadequate response of the sphincter to rectal distension might be responsible for the obstructive symptoms in these diseases."}
{"id": "PMID:470255", "title": "A case of double pylorus.", "content": "Doubling of the pyloric channel is one of the rare anomalies of the intestinal tract. There have been 23 cases of double pylorus that we have been able to find in the literature. As the etiologic aspects of double pylorus the majorities of the cases were thought to be acquired lesions from ulcer disease except three cases. We present a case of double pylorus which seems to be acquired lesion, with a review of the literature.", "contents": "A case of double pylorus. Doubling of the pyloric channel is one of the rare anomalies of the intestinal tract. There have been 23 cases of double pylorus that we have been able to find in the literature. As the etiologic aspects of double pylorus the majorities of the cases were thought to be acquired lesions from ulcer disease except three cases. We present a case of double pylorus which seems to be acquired lesion, with a review of the literature."}
{"id": "PMID:470256", "title": "Congenital pyloric atresia--a report of two cases.", "content": "Two cases of the membranous type of pyloric atresia are described and a review of 99 cases reported in the literature is presented. Excision and incision of diaphragm with pyloroplasty was performed on both cases. The first patient was alive following the second operation of gastroduodenostomy for postoperative stenosis. The second premature patient died of sepsis on the 58th postoperative day. The treatment should be surgical and the operative procedure should be chosen depending on the type of atresia. Excision or incision of the diaphragm with pyloroplasty and gastroduodenostomy are the procedures of choice. Gastrojejunostomy should be avoided because of its high mortality rate.", "contents": "Congenital pyloric atresia--a report of two cases. Two cases of the membranous type of pyloric atresia are described and a review of 99 cases reported in the literature is presented. Excision and incision of diaphragm with pyloroplasty was performed on both cases. The first patient was alive following the second operation of gastroduodenostomy for postoperative stenosis. The second premature patient died of sepsis on the 58th postoperative day. The treatment should be surgical and the operative procedure should be chosen depending on the type of atresia. Excision or incision of the diaphragm with pyloroplasty and gastroduodenostomy are the procedures of choice. Gastrojejunostomy should be avoided because of its high mortality rate."}
{"id": "PMID:470274", "title": "The recognition of infective endocarditis.", "content": "Patients with infective endocarditis are frequently first evaluated in an emergency department at a time when clinical data are insufficient for a conclusive diagnosis. The records of 22 patients with proven bacterial endocarditis first seen in our emergency department were reviewed. The most common feature of the initial history was the presence of fever in 11 (50%). The most common physical finding was a heart murmur in 18 (82%). All patients had positive blood cultures. The most frequently isolated organism was Staphylococcus aureus. Pertinent clinical and laboratory data available on admission that enabled the emergency physician to suspect infective endocarditis were systemic manifestations of fever, malaise and arthralgias together with anemia, leukocytosis and hematuria.", "contents": "The recognition of infective endocarditis. Patients with infective endocarditis are frequently first evaluated in an emergency department at a time when clinical data are insufficient for a conclusive diagnosis. The records of 22 patients with proven bacterial endocarditis first seen in our emergency department were reviewed. The most common feature of the initial history was the presence of fever in 11 (50%). The most common physical finding was a heart murmur in 18 (82%). All patients had positive blood cultures. The most frequently isolated organism was Staphylococcus aureus. Pertinent clinical and laboratory data available on admission that enabled the emergency physician to suspect infective endocarditis were systemic manifestations of fever, malaise and arthralgias together with anemia, leukocytosis and hematuria."}
{"id": "PMID:470275", "title": "Anxiety in ambulatory emergency patients.", "content": "The anxiety level of ambulatory emergency department patients without a diagnosis of a physical illness was studied. These patients were compared to a group with a diagnosis of nontraumatic surgical disease. Both the study group and the comparison group were found to have elevated anxiety levels. In addition, those with nonphysical illness had elevated levels of covert anxiety when compared to their overt levels (p less than .03) and when contrasted with the comparison group's covert levels ( p less than .03). In the treatment of all a patient's problems the psychosomatic illnesses evidenced by raised covert anxiety levels may require intensive treatment both within and without the emergency department.", "contents": "Anxiety in ambulatory emergency patients. The anxiety level of ambulatory emergency department patients without a diagnosis of a physical illness was studied. These patients were compared to a group with a diagnosis of nontraumatic surgical disease. Both the study group and the comparison group were found to have elevated anxiety levels. In addition, those with nonphysical illness had elevated levels of covert anxiety when compared to their overt levels (p less than .03) and when contrasted with the comparison group's covert levels ( p less than .03). In the treatment of all a patient's problems the psychosomatic illnesses evidenced by raised covert anxiety levels may require intensive treatment both within and without the emergency department."}
{"id": "PMID:470276", "title": "Socioeconomic aspects of stab and gunshot wounds.", "content": "Records of 294 patients with gunshot, stab, or shotgun wounds admitted to Charlotte Memorial Hospital and Medical Center between July 1, 1976 and June 30, 1977 were reviewed. Data were gathered on 24 clinical, social, and economic aspects to discover any patterns in these areas. Eighty-three percent of the patients were male; 71%, black; 70%, unmarried; and 56%, between 16 and 30 years of age. Fifty-five percent came to the hospital between 10 pm and 3 am; 57% on Friday, Saturday, or Sunday; 31% came in June, July, or August. Sixty-four percent were gunshot wounds, 80% of which were by small caliber weapons. Ninety-four percent of all patients survived. The patients \"consumed\" 3,260 hosptial days, 526 intensive care unit days, 533 units of blood, and 261 operations, totaling 595 hours of operating room time. Hospital charges totaled $668,000, of which 1.1% was paid from private funds and nearly 65% from the taxpayers and other private paying patients.", "contents": "Socioeconomic aspects of stab and gunshot wounds. Records of 294 patients with gunshot, stab, or shotgun wounds admitted to Charlotte Memorial Hospital and Medical Center between July 1, 1976 and June 30, 1977 were reviewed. Data were gathered on 24 clinical, social, and economic aspects to discover any patterns in these areas. Eighty-three percent of the patients were male; 71%, black; 70%, unmarried; and 56%, between 16 and 30 years of age. Fifty-five percent came to the hospital between 10 pm and 3 am; 57% on Friday, Saturday, or Sunday; 31% came in June, July, or August. Sixty-four percent were gunshot wounds, 80% of which were by small caliber weapons. Ninety-four percent of all patients survived. The patients \"consumed\" 3,260 hosptial days, 526 intensive care unit days, 533 units of blood, and 261 operations, totaling 595 hours of operating room time. Hospital charges totaled $668,000, of which 1.1% was paid from private funds and nearly 65% from the taxpayers and other private paying patients."}
{"id": "PMID:470277", "title": "Physician and patient acceptance of emergency nurse practitioners.", "content": "Patient and physician attitudes toward emergency nurse practitioners (ENP) were surveyed in our emergency medical service. The responses to the questionnaires indicated a high degree of satisfaction with the ENP similar to that reported in a survey of patient reaction to physician assistants (PA) in the emergency department. In our emergency medical service, physicians expressed a concern over the practitioner's care of the emergent patient. Their confidence in the practitioner's clinical judgment was greater in their care of nonurgent and urgent patients.", "contents": "Physician and patient acceptance of emergency nurse practitioners. Patient and physician attitudes toward emergency nurse practitioners (ENP) were surveyed in our emergency medical service. The responses to the questionnaires indicated a high degree of satisfaction with the ENP similar to that reported in a survey of patient reaction to physician assistants (PA) in the emergency department. In our emergency medical service, physicians expressed a concern over the practitioner's care of the emergent patient. Their confidence in the practitioner's clinical judgment was greater in their care of nonurgent and urgent patients."}
{"id": "PMID:470278", "title": "Esophageal perforation secondary to alkaline battery ingestion.", "content": "A 16-month-old infant ingested an alkaline battery, 22 mm x 5 mm, which became entrapped in her esophagus. She was brought to the emergency department with a four-day history of vomiting. After tube thoracostomy, the battery was removed by esophagoscopy. However, she experienced cardiac arrest in the intensive care unit. Post mortem examination revealed exsanguination by perforation of the posterior aspect of the aortic arch as the cause of death. Microscopic examination showed liquefaction necrosis through the mucosa and submucosa of the esophagus into the upper mediastinum and right pleural space. The possible role of the alkaline center of the battery causing perforation is presented.", "contents": "Esophageal perforation secondary to alkaline battery ingestion. A 16-month-old infant ingested an alkaline battery, 22 mm x 5 mm, which became entrapped in her esophagus. She was brought to the emergency department with a four-day history of vomiting. After tube thoracostomy, the battery was removed by esophagoscopy. However, she experienced cardiac arrest in the intensive care unit. Post mortem examination revealed exsanguination by perforation of the posterior aspect of the aortic arch as the cause of death. Microscopic examination showed liquefaction necrosis through the mucosa and submucosa of the esophagus into the upper mediastinum and right pleural space. The possible role of the alkaline center of the battery causing perforation is presented."}
{"id": "PMID:470279", "title": "Central retinal artery occlusion.", "content": "Central retinal artery occlusion is characterized by sudden, painless visual loss due to blockage of retinal blood flow. It has been reported in all age groups including children but is most frequent in the sixth decade. Both thrombosis and embolism have been suggested as possible mechanisms. The emergency treatment includes intravenous acetazolamide, digital ocular massage, and inhalation of 5% carbon dioxide -- 95% oxygen for ten minutes. Following emergency treatment, immediate ophthalmologic referral and consultation is required. Comprehensive medical evaluation should be initiated to exclude systemic vascular disease.", "contents": "Central retinal artery occlusion. Central retinal artery occlusion is characterized by sudden, painless visual loss due to blockage of retinal blood flow. It has been reported in all age groups including children but is most frequent in the sixth decade. Both thrombosis and embolism have been suggested as possible mechanisms. The emergency treatment includes intravenous acetazolamide, digital ocular massage, and inhalation of 5% carbon dioxide -- 95% oxygen for ten minutes. Following emergency treatment, immediate ophthalmologic referral and consultation is required. Comprehensive medical evaluation should be initiated to exclude systemic vascular disease."}
{"id": "PMID:470280", "title": "Hiccups: an unusual manifestation of an abdominal aortic aneurysm.", "content": "A patient with hiccups was found to have an abdominal aortic aneurysm that subsequently ruptured. We believe that a leaking abdominal aortic aneurysm led to an ileus-induced distention of the splenic flexure of the colon with consequent diaphragmatic irritation and phrenic nerve stimulation. This led to persistent hiccups as a result of repetitive stimulation of the reflex arc mediating hiccups. Persistent hiccups require investigation for an underlying organic etiology, and a leaking abdominal aortic aneurysm should be included in the differential diagnosis.", "contents": "Hiccups: an unusual manifestation of an abdominal aortic aneurysm. A patient with hiccups was found to have an abdominal aortic aneurysm that subsequently ruptured. We believe that a leaking abdominal aortic aneurysm led to an ileus-induced distention of the splenic flexure of the colon with consequent diaphragmatic irritation and phrenic nerve stimulation. This led to persistent hiccups as a result of repetitive stimulation of the reflex arc mediating hiccups. Persistent hiccups require investigation for an underlying organic etiology, and a leaking abdominal aortic aneurysm should be included in the differential diagnosis."}
{"id": "PMID:470281", "title": "Clear view of the index sesamoid: a sign of irreducible metacarpophalangeal joint dislocation.", "content": "If the index sesamoid bone is clearly seen in the joint following significant injury to the index metacarpophalangeal joint on radiographic examination, there is the probability of incarceration of the volar plate. In almost all cases, an open reduction will be necessary to re-establish the normal relationship between the second metacarpal and the proximal phalanx. In a case of irreducible dislocation of the index metacarpophalangeal joint, attempted reduction caused the injury to appear as a subluxation.", "contents": "Clear view of the index sesamoid: a sign of irreducible metacarpophalangeal joint dislocation. If the index sesamoid bone is clearly seen in the joint following significant injury to the index metacarpophalangeal joint on radiographic examination, there is the probability of incarceration of the volar plate. In almost all cases, an open reduction will be necessary to re-establish the normal relationship between the second metacarpal and the proximal phalanx. In a case of irreducible dislocation of the index metacarpophalangeal joint, attempted reduction caused the injury to appear as a subluxation."}
{"id": "PMID:470289", "title": "Preliminary report: the role of short-leg, tone-reducing casts as an adjunct to physical therapy of patients with cerebral palsy.", "content": "In the past two years, we have used specially constructed, short-leg casts as an adjunct to the therapy program for 99 patients with cerebral palsy. The casts provide tone reduction and increased stability, while allowing mobility, and have been useful in initiating weight-bearing activities and improvement of gait pattern.", "contents": "Preliminary report: the role of short-leg, tone-reducing casts as an adjunct to physical therapy of patients with cerebral palsy. In the past two years, we have used specially constructed, short-leg casts as an adjunct to the therapy program for 99 patients with cerebral palsy. The casts provide tone reduction and increased stability, while allowing mobility, and have been useful in initiating weight-bearing activities and improvement of gait pattern."}
{"id": "PMID:470291", "title": "Atlanto-occipital instability as a result of pituitary adenoma: case report with successful management by cervico-occipital fusion.", "content": "A 48-year-old woman with an extensive pituitary adenoma developed atlanto-occipital instability secondary to erosion of the base of the skull by the tumor. The pathophysiology, diagnosis and surgical treatment are described.", "contents": "Atlanto-occipital instability as a result of pituitary adenoma: case report with successful management by cervico-occipital fusion. A 48-year-old woman with an extensive pituitary adenoma developed atlanto-occipital instability secondary to erosion of the base of the skull by the tumor. The pathophysiology, diagnosis and surgical treatment are described."}
{"id": "PMID:470292", "title": "Skeletal changes during prolonged external irradiation: alterations in marrow, growth plate and osteoclast populations.", "content": "This report describes hematologic and skeletal changes in young mice subjected to continuous external whole body irradiation (45 rads/day) for 4 days to 12 weeks. Irradiation caused a rapid depletion of hematopoietic stem cells, marrow aplasia and pancytopenia, all of which persisted during the period of irradiation but resolved afterward. In spite of suppressed cellular proliferation and disarray of cartilage cell columns in metaphyseal plates, linear bone growth appeared to continue at physiologic rates. Histologic and morphometric studies provided no evidence of impaired osteoblast function, but the presence of thickended trabeculae beneath the growth plate and of cartilagenous islands within cortical shafts of long bones indicated that bone remodeling was deficient. Direct osteoclast counts demonstrated that marrow aplasia was followed by a progressive decline that could not be reversed by parathormone injections or infusions with mature macrophages and lymphocytes but that resolved once the bone marrow recovered following cessation of irradiation. Therefore, the altered bone remodeling probably resulted from radiation injury to osteoclast precursors in the hematopoietic compartment.", "contents": "Skeletal changes during prolonged external irradiation: alterations in marrow, growth plate and osteoclast populations. This report describes hematologic and skeletal changes in young mice subjected to continuous external whole body irradiation (45 rads/day) for 4 days to 12 weeks. Irradiation caused a rapid depletion of hematopoietic stem cells, marrow aplasia and pancytopenia, all of which persisted during the period of irradiation but resolved afterward. In spite of suppressed cellular proliferation and disarray of cartilage cell columns in metaphyseal plates, linear bone growth appeared to continue at physiologic rates. Histologic and morphometric studies provided no evidence of impaired osteoblast function, but the presence of thickended trabeculae beneath the growth plate and of cartilagenous islands within cortical shafts of long bones indicated that bone remodeling was deficient. Direct osteoclast counts demonstrated that marrow aplasia was followed by a progressive decline that could not be reversed by parathormone injections or infusions with mature macrophages and lymphocytes but that resolved once the bone marrow recovered following cessation of irradiation. Therefore, the altered bone remodeling probably resulted from radiation injury to osteoclast precursors in the hematopoietic compartment."}
{"id": "PMID:470293", "title": "An assistive equipment controller for quadriplegics.", "content": "A joystick is traditionally used to manipulate electrically powered wheelchairs and radio-controlled model aeroplanes. Extensive modifications of the joystick have resulted in the development of an inconspicuous chin-operated control; the latter enables quadriplegics with high-level spinal cord injuries to operate not only electric wheelchairs but also a complete range of other assistive devices, including robotic arm and environmental control systems currently under development. Through the use of the chin-operated control such quadriplegics can manipulate any of these devices or systems without the help of an attendant and without interfering with the use of a mouthstick.", "contents": "An assistive equipment controller for quadriplegics. A joystick is traditionally used to manipulate electrically powered wheelchairs and radio-controlled model aeroplanes. Extensive modifications of the joystick have resulted in the development of an inconspicuous chin-operated control; the latter enables quadriplegics with high-level spinal cord injuries to operate not only electric wheelchairs but also a complete range of other assistive devices, including robotic arm and environmental control systems currently under development. Through the use of the chin-operated control such quadriplegics can manipulate any of these devices or systems without the help of an attendant and without interfering with the use of a mouthstick."}
{"id": "PMID:470294", "title": "Extremity fractures in children with neuromuscular disease.", "content": "This report discusses the occurrence and management of fractures seen in 30 children with neuromuscular disorders. Twenty fractures were seen in 17 ambulatory patients, ages 5 to 16. Twelve of the patients in this group had Duchenne muscular dystrophy (DMD), 3 had spinal muscular atrophy (SMA) and 2 had Charcot--Marie--Tooth (CMT) disease. Treatment consists of reduction of the fractures followed by immobilization of the extremities; however, continuation of everyday activities, including ambulation, should be emphasized. In fact, five patients ceased walking as a direct result of immobilization following fracture. Sixteen fractures were seen in 13 non-ambulatory patients, ages 4 to 19. These fractures were generally caused by very small forces; the fractures were rarely displaced. Eight of the fractures in osteoporotic and contracted knee joints occurred in either the distal femur or proximal tibia. Functional loss can be minimized by splinting until the patient no longer complains of pain. Splinting should be followed by rapid return to full activity.", "contents": "Extremity fractures in children with neuromuscular disease. This report discusses the occurrence and management of fractures seen in 30 children with neuromuscular disorders. Twenty fractures were seen in 17 ambulatory patients, ages 5 to 16. Twelve of the patients in this group had Duchenne muscular dystrophy (DMD), 3 had spinal muscular atrophy (SMA) and 2 had Charcot--Marie--Tooth (CMT) disease. Treatment consists of reduction of the fractures followed by immobilization of the extremities; however, continuation of everyday activities, including ambulation, should be emphasized. In fact, five patients ceased walking as a direct result of immobilization following fracture. Sixteen fractures were seen in 13 non-ambulatory patients, ages 4 to 19. These fractures were generally caused by very small forces; the fractures were rarely displaced. Eight of the fractures in osteoporotic and contracted knee joints occurred in either the distal femur or proximal tibia. Functional loss can be minimized by splinting until the patient no longer complains of pain. Splinting should be followed by rapid return to full activity."}
{"id": "PMID:470295", "title": "Iatrogenic compression of the thoracic outlet.", "content": "Arterial injury and brachial plexus nerve compression have been reported in association with surgical procedures in the area of the thoracic outlet. An initial report of iatrogenic injury was related to the operative correction of Sprengel's deformity, which required lowering of the scapula in a child. Compression of the neurovascular bundle between the clavicle and the first rib occurred during this surgical migration. The developmental anatomy of the costo-clavicular area and the descent of the upper limb from its initial cervical position are reviewed. This relationship between the clavicle and the first rib may also be altered by surgical procedures carried out on adults. Two cases of the iatrogenic injury to the brachial plexus are reported in association with operations performed in the area of the clavicle. Correction of these injuries resulted when the costoclavicular space was re-established.", "contents": "Iatrogenic compression of the thoracic outlet. Arterial injury and brachial plexus nerve compression have been reported in association with surgical procedures in the area of the thoracic outlet. An initial report of iatrogenic injury was related to the operative correction of Sprengel's deformity, which required lowering of the scapula in a child. Compression of the neurovascular bundle between the clavicle and the first rib occurred during this surgical migration. The developmental anatomy of the costo-clavicular area and the descent of the upper limb from its initial cervical position are reviewed. This relationship between the clavicle and the first rib may also be altered by surgical procedures carried out on adults. Two cases of the iatrogenic injury to the brachial plexus are reported in association with operations performed in the area of the clavicle. Correction of these injuries resulted when the costoclavicular space was re-established."}
{"id": "PMID:470296", "title": "The surgical approach to refractory trochanteric bursitis.", "content": "This article identifies and describes a small group of patients with trochanteric bursitis for whom all modes of conservative therapy have failed. They are treated surgically by release of the ileotibial band, removal of trochanteric osteophytes and debridement of the gluteus maximus bursae. In this series all of the patients had satisfactory results and returned to near-normal function without significant complications.", "contents": "The surgical approach to refractory trochanteric bursitis. This article identifies and describes a small group of patients with trochanteric bursitis for whom all modes of conservative therapy have failed. They are treated surgically by release of the ileotibial band, removal of trochanteric osteophytes and debridement of the gluteus maximus bursae. In this series all of the patients had satisfactory results and returned to near-normal function without significant complications."}
{"id": "PMID:470311", "title": "[Method of cardiac stimulation in sick sinus syndrome].", "content": "The condition of the conducting system of the heart and the sinus node recovery time were studied in 27 patients with the cardiac rhythm pathology under consideration. On the grounds of the resultant data the question concerning the implantation of this or that type of pacemaker (operating in a fixed regimen or \"on demand\") and-the positioning of the fixing electrode in the atria or ventricle was resolved. The advantages and shortcomings of each of the suggested methods for surgical treatment of sick sinus node are discussed.", "contents": "[Method of cardiac stimulation in sick sinus syndrome]. The condition of the conducting system of the heart and the sinus node recovery time were studied in 27 patients with the cardiac rhythm pathology under consideration. On the grounds of the resultant data the question concerning the implantation of this or that type of pacemaker (operating in a fixed regimen or \"on demand\") and-the positioning of the fixing electrode in the atria or ventricle was resolved. The advantages and shortcomings of each of the suggested methods for surgical treatment of sick sinus node are discussed."}
{"id": "PMID:470314", "title": "[Myocardial scintigraphy in the diagnosis of infarct in patients with disorders of intraventricular conduction].", "content": "Myocardial scintigraphy with pyrophosphate-99mTc-Sn was used as a diagnostic test in 23 patients with different disorders of rhythm and intracardia conduction who were admitted to the department of emergency cardiology of the All-Union Cardiological Scientific Center, Academy of Medical Sciences of the USSR with suspected acute myocardial infarction. Scintigrams were positive in 11 of 12 patients with acute myocardial infarction who were examined in periods of 6 hours of 10 days. One patient examined on the 13th day of the disease had negative scintigrams. In 6 of 11 patients with myocardial infarction not confirmed, the scintigrams were also negative, in 3 other patients positive scintigrams were due to a postinfarction aneurysm. The authors conclude that myocardial scintigraphy with pyrophosphate-99mTc-Sn is an adequate test in the diagnosis of acute myocardial infarction, especially in the examination of patients with disorders of intracardiac conduction.", "contents": "[Myocardial scintigraphy in the diagnosis of infarct in patients with disorders of intraventricular conduction]. Myocardial scintigraphy with pyrophosphate-99mTc-Sn was used as a diagnostic test in 23 patients with different disorders of rhythm and intracardia conduction who were admitted to the department of emergency cardiology of the All-Union Cardiological Scientific Center, Academy of Medical Sciences of the USSR with suspected acute myocardial infarction. Scintigrams were positive in 11 of 12 patients with acute myocardial infarction who were examined in periods of 6 hours of 10 days. One patient examined on the 13th day of the disease had negative scintigrams. In 6 of 11 patients with myocardial infarction not confirmed, the scintigrams were also negative, in 3 other patients positive scintigrams were due to a postinfarction aneurysm. The authors conclude that myocardial scintigraphy with pyrophosphate-99mTc-Sn is an adequate test in the diagnosis of acute myocardial infarction, especially in the examination of patients with disorders of intracardiac conduction."}
{"id": "PMID:470315", "title": "[Lidocaine pharmacokinetics and substantiation of the new method of its administration in myocardial infarct].", "content": "Lidocain pharmacokinetics was studied in 12 patients with acute myocardial infarction (males and females from 40 to 90 years of age with obvious disorders of hepatic and kidney function or severe hemodynamic disorders). In ten of them the main pharmacokinetic parameters were determined; the average value of lidocain clearance was 0.45 1/min. The optimum rate of continuous drip infusion was calculated (1.35 mg/min). It is shown that the existing method of lidocain administration by the \"initial dose + infusion\" schedule is unsatisfactory from the pharmacokinetic standpoint. Recommendations are given for the choice of the optimum concentrations of lidocain solution used in different rates of continuous infusion.", "contents": "[Lidocaine pharmacokinetics and substantiation of the new method of its administration in myocardial infarct]. Lidocain pharmacokinetics was studied in 12 patients with acute myocardial infarction (males and females from 40 to 90 years of age with obvious disorders of hepatic and kidney function or severe hemodynamic disorders). In ten of them the main pharmacokinetic parameters were determined; the average value of lidocain clearance was 0.45 1/min. The optimum rate of continuous drip infusion was calculated (1.35 mg/min). It is shown that the existing method of lidocain administration by the \"initial dose + infusion\" schedule is unsatisfactory from the pharmacokinetic standpoint. Recommendations are given for the choice of the optimum concentrations of lidocain solution used in different rates of continuous infusion."}
{"id": "PMID:470316", "title": "[Effect of digoxin on central hemodynamics in myocardial infarct].", "content": "The effect of digoxin on the cardiac index and diastolic pressure in the pulmonary artery was studied in 17 patients with acute myocardial infarction on the 1st-3rd day of the disease by the method of thermodilution. Twelve of these patients had clinical manifestations of congestive circulatory insufficiency. Digoxin was infused intravenously in a dose of 0.5 mg. Its infusion led to reduction of diastolic pressure in the pulmonary artery from 19.0 to 16.7 mm Hg and rise of cardiac index from 2.26 to 2.4 1/min/m2 (statistically untrustworhty difference). In all cases digoxin concentration in blood serum examined by the radioimmune method was therapeutical (1.64 ng/ml, on the average). The positive hemodynamic effect of digoxin infused in a dose producing its therapeutic concentration in blood is negligible.", "contents": "[Effect of digoxin on central hemodynamics in myocardial infarct]. The effect of digoxin on the cardiac index and diastolic pressure in the pulmonary artery was studied in 17 patients with acute myocardial infarction on the 1st-3rd day of the disease by the method of thermodilution. Twelve of these patients had clinical manifestations of congestive circulatory insufficiency. Digoxin was infused intravenously in a dose of 0.5 mg. Its infusion led to reduction of diastolic pressure in the pulmonary artery from 19.0 to 16.7 mm Hg and rise of cardiac index from 2.26 to 2.4 1/min/m2 (statistically untrustworhty difference). In all cases digoxin concentration in blood serum examined by the radioimmune method was therapeutical (1.64 ng/ml, on the average). The positive hemodynamic effect of digoxin infused in a dose producing its therapeutic concentration in blood is negligible."}
{"id": "PMID:470317", "title": "[Role of changes in rheological properties of blood in the pathogenesis of disorders of cardiac rhythm and conductivity].", "content": "Changes were induced in the rheological blood properties and hemostasis in 40 albino rats by intracardiac injection of high-molecular dextran, arvin, and ethanol. Ten rats received dipyridamole. Changes caused by these agents in erythrocyte deformability were determined by the rheoscopical method. Their effect on the threshold of ventricular fibrillation induced by electric current was also determined. Dextran and arvin reduced erythrocyte deformability and the threshold of ventricular fibrillation to half the initial values. An increase in the ventricular fibrillation threshold due to the effect of dipyridamole was attended by improvement in erythrocyte deformability. Ethanol reduced the threshold of ventricular fibrillation and caused disorders of cardiac atrioventricular conduction. Intravenous infusion of high-molecular dextran and arvin caused various disorders of cardiac rhythm and atrioventricular conduction in 11 out of 12 rabbits. Preliminary administration of rheopolyglucin in 3 rabbits prevented the development of cardiac arrhythmias on injection of dextran and arvin. The authors believe that deterioration of the blood rheological properties is one of the pathogenetic mechanisms in the development of disorders of cardiac rhythm and conduction.", "contents": "[Role of changes in rheological properties of blood in the pathogenesis of disorders of cardiac rhythm and conductivity]. Changes were induced in the rheological blood properties and hemostasis in 40 albino rats by intracardiac injection of high-molecular dextran, arvin, and ethanol. Ten rats received dipyridamole. Changes caused by these agents in erythrocyte deformability were determined by the rheoscopical method. Their effect on the threshold of ventricular fibrillation induced by electric current was also determined. Dextran and arvin reduced erythrocyte deformability and the threshold of ventricular fibrillation to half the initial values. An increase in the ventricular fibrillation threshold due to the effect of dipyridamole was attended by improvement in erythrocyte deformability. Ethanol reduced the threshold of ventricular fibrillation and caused disorders of cardiac atrioventricular conduction. Intravenous infusion of high-molecular dextran and arvin caused various disorders of cardiac rhythm and atrioventricular conduction in 11 out of 12 rabbits. Preliminary administration of rheopolyglucin in 3 rabbits prevented the development of cardiac arrhythmias on injection of dextran and arvin. The authors believe that deterioration of the blood rheological properties is one of the pathogenetic mechanisms in the development of disorders of cardiac rhythm and conduction."}
{"id": "PMID:470318", "title": "[Competitive rhythm as a complication of asynchronous electric cardiac stimulation].", "content": "The article analyses arrhythmias recorded in the hospital period in patients with various disorders of atrioventricular conduction in continous asynchronous stimulation of the heart. The frequency with which the competitive rhythm occurred in implantation of 134 asynchronous cardiostimulants was 11.9%. Mortality from its development was 18.8%. The authors believe unjustified widening of the scope of indications for asynchronous stimulation of the heart to be one of the main causes of parasystole. Strict adherence to these indications in conducive to marked decrease in the incidence of this complication but does not rule it out completely. Implantation of biocontrolled cardiostimulants, in cases with corresponding indications, is the optimum method for the prevention of competitive rhythm.", "contents": "[Competitive rhythm as a complication of asynchronous electric cardiac stimulation]. The article analyses arrhythmias recorded in the hospital period in patients with various disorders of atrioventricular conduction in continous asynchronous stimulation of the heart. The frequency with which the competitive rhythm occurred in implantation of 134 asynchronous cardiostimulants was 11.9%. Mortality from its development was 18.8%. The authors believe unjustified widening of the scope of indications for asynchronous stimulation of the heart to be one of the main causes of parasystole. Strict adherence to these indications in conducive to marked decrease in the incidence of this complication but does not rule it out completely. Implantation of biocontrolled cardiostimulants, in cases with corresponding indications, is the optimum method for the prevention of competitive rhythm."}
{"id": "PMID:470320", "title": "[Recording the bundle of His potential from the chest surface].", "content": "An attempt was made to record the bundle of His potential from the chest surface in 38 persons by means of definite filtration, intensification, and averaging of the ECG. Three electrode leads were used for each person examined: transthoracic, horizontal, and on the longitudinal heart axis. In 34 persons (89%) the potential was recorded in the P--Q interval, its width and distance from the QRS complex corresponded to the parameters of the bundle of His potential. The potential was recorded most frequently in the transthoracic lead (50%).", "contents": "[Recording the bundle of His potential from the chest surface]. An attempt was made to record the bundle of His potential from the chest surface in 38 persons by means of definite filtration, intensification, and averaging of the ECG. Three electrode leads were used for each person examined: transthoracic, horizontal, and on the longitudinal heart axis. In 34 persons (89%) the potential was recorded in the P--Q interval, its width and distance from the QRS complex corresponded to the parameters of the bundle of His potential. The potential was recorded most frequently in the transthoracic lead (50%)."}
{"id": "PMID:470321", "title": "[Direct recording of electrical activity of human sinoatrial node].", "content": "The authors developed a method for recording the electrical activity directly from the human sinus node through the right subclavian vein. Electrical activity of the sinoatrial node (SA) was studied in 179 patients with CHD (angina pectoris, myocardial infarction). The sick-sinus syndrome (SSS) was revealed in 86 patients. The duration of slow deflection Sd, Ss -- P interval (from the onset of Ss to the onset of P wave), P -- A1 interval (from the onset of P wave to the first atrial spike) was prolonged in cases with SSS. The duration of Sd, Ss -- P and P -- A1 decreased after nitroglycerine and atropine administration but increased after benzodixin administration. The data on direct pacing of the SA node and the changes in the duration of phase Sd and Ss after the administration of drugs confirm that slow deflection Sd and rapid preatrial deflection Ss reflect the electrical activity of the SA node.", "contents": "[Direct recording of electrical activity of human sinoatrial node]. The authors developed a method for recording the electrical activity directly from the human sinus node through the right subclavian vein. Electrical activity of the sinoatrial node (SA) was studied in 179 patients with CHD (angina pectoris, myocardial infarction). The sick-sinus syndrome (SSS) was revealed in 86 patients. The duration of slow deflection Sd, Ss -- P interval (from the onset of Ss to the onset of P wave), P -- A1 interval (from the onset of P wave to the first atrial spike) was prolonged in cases with SSS. The duration of Sd, Ss -- P and P -- A1 decreased after nitroglycerine and atropine administration but increased after benzodixin administration. The data on direct pacing of the SA node and the changes in the duration of phase Sd and Ss after the administration of drugs confirm that slow deflection Sd and rapid preatrial deflection Ss reflect the electrical activity of the SA node."}
{"id": "PMID:470322", "title": "[Treatment of acute left-ventricular insufficiency complicated by tachyarrhythmia under conditions of cardiological emergency service].", "content": "The article analyses 188 cases treated for acute left ventricular insufficiency (ALVI) in different forms of tachyarrhythmia. It was established that in patients with supraventricular paroxysmal tachycardia ALVI develops when the rate of cardiac contractions is 180 +/- 3/min on the average, whereas in patients with cardiac fibrillation it develops when the rate of contractions is 163 +/- 4/min. For successful treatment of ALVI of a tachyarrhythmic character it is necessary to restore the sinus rhythm or slow down the rhythm of cardiac contractions in patients with supraventricular paroxysmal tachycardia to 113 +/- 5/min on the average and in patients with cardiac fibrillation to 118 +/- 2/min. Isoptin and obsidan produce the highest decelerating effect. In half of the patients with tachyarrhythmia. ALVI was relieved by means of antiarrhythmic agents among which obsidan and isoptin proved most effective.", "contents": "[Treatment of acute left-ventricular insufficiency complicated by tachyarrhythmia under conditions of cardiological emergency service]. The article analyses 188 cases treated for acute left ventricular insufficiency (ALVI) in different forms of tachyarrhythmia. It was established that in patients with supraventricular paroxysmal tachycardia ALVI develops when the rate of cardiac contractions is 180 +/- 3/min on the average, whereas in patients with cardiac fibrillation it develops when the rate of contractions is 163 +/- 4/min. For successful treatment of ALVI of a tachyarrhythmic character it is necessary to restore the sinus rhythm or slow down the rhythm of cardiac contractions in patients with supraventricular paroxysmal tachycardia to 113 +/- 5/min on the average and in patients with cardiac fibrillation to 118 +/- 2/min. Isoptin and obsidan produce the highest decelerating effect. In half of the patients with tachyarrhythmia. ALVI was relieved by means of antiarrhythmic agents among which obsidan and isoptin proved most effective."}
{"id": "PMID:470323", "title": "[Syndrome of premature ventricular repolarization].", "content": "ECG abnormalities are described in 71 persons with the syndrome of premature repolarization of the ventricles. The syndrome of premature ventricular repolarization is considered a benign, most probably congenital, change of the electro-physiological state of the heart which due to its morphological peculiarities, however, leads to hyperdiagnosis of ischemic heart disease. In the discussion of the pathogenesis of this syndrome, the main attention is focused on the increased effect of the vagus nerve and the possible existence of anomalous additional pathways of atrioventricular conduction.", "contents": "[Syndrome of premature ventricular repolarization]. ECG abnormalities are described in 71 persons with the syndrome of premature repolarization of the ventricles. The syndrome of premature ventricular repolarization is considered a benign, most probably congenital, change of the electro-physiological state of the heart which due to its morphological peculiarities, however, leads to hyperdiagnosis of ischemic heart disease. In the discussion of the pathogenesis of this syndrome, the main attention is focused on the increased effect of the vagus nerve and the possible existence of anomalous additional pathways of atrioventricular conduction."}
{"id": "PMID:470324", "title": "[Effect of emotional stress on the contractile function of human heart (according to the echocardiographic data)].", "content": "Cardiac function was studied by echocardiography in 12 healthy sixth-year students at mid-semester, immediately before taking an exam and 30 minutes after it. It was found that the emotional stress experienced prior to the exam is attended with an increase in cardiac output by one and a half times and external work by two. This increase of the pump cardiac function is ensured without any essential increase in the diastolic dimensions of the left cardiac ventricle at the expense of fuller blood ejection from the left ventricle and decrease of its residual systolic volume. Since the students had taken more than 50 exams in the past and were adapted to the pre-examination situation, the circulatory changes revealed may be regarded as adaptive providing for the rich flow of blood to the brain.", "contents": "[Effect of emotional stress on the contractile function of human heart (according to the echocardiographic data)]. Cardiac function was studied by echocardiography in 12 healthy sixth-year students at mid-semester, immediately before taking an exam and 30 minutes after it. It was found that the emotional stress experienced prior to the exam is attended with an increase in cardiac output by one and a half times and external work by two. This increase of the pump cardiac function is ensured without any essential increase in the diastolic dimensions of the left cardiac ventricle at the expense of fuller blood ejection from the left ventricle and decrease of its residual systolic volume. Since the students had taken more than 50 exams in the past and were adapted to the pre-examination situation, the circulatory changes revealed may be regarded as adaptive providing for the rich flow of blood to the brain."}
{"id": "PMID:470325", "title": "[Antiaccelerative and antiarrhythmic effect of veratramin on myocardial cells in culture].", "content": "It is shown that veratramin (2.5 X 10(-5)M) inhibits, partially or completely, the accelerative effect of adrenalin (5.5 X 10(-7) M), isoproterenol (10(-7) M), prostaglandin E2 (5 X 10(-5) M), and glucagon (10(-5) M) in experiments on cultures of newborn rat cardiac cells and the effect of histamine on 3--4-week embryo cultures. Veratramin (1.25--25 X 10(-5) M) arrests digitoxin-induced (1.25 X10(-5) M--5 X 10(-5) M) fibrillation of individual cells and of cell groups. Veratramin alone and in combination with adrenalin does not change the inotropic parameters of individual cells. Bearing in mind that veratramin (2.5 X 10(-5) M) inhibits the accelerative effect of substances which activate and those which do not activate adenylatecyclse, as well as the fact that it has no effect on the content of cAMP in cultures and does not correct the increase in the cAMP content induced by adrenalin (5.5 X 10(-7) M), the authors conclude that the chronotropic effect of veratramin is not associated with the cell receptor mechanisms but is due to its effect on the transport of ions through the cell membrane, which is a responsible process in the generation of impulses.", "contents": "[Antiaccelerative and antiarrhythmic effect of veratramin on myocardial cells in culture]. It is shown that veratramin (2.5 X 10(-5)M) inhibits, partially or completely, the accelerative effect of adrenalin (5.5 X 10(-7) M), isoproterenol (10(-7) M), prostaglandin E2 (5 X 10(-5) M), and glucagon (10(-5) M) in experiments on cultures of newborn rat cardiac cells and the effect of histamine on 3--4-week embryo cultures. Veratramin (1.25--25 X 10(-5) M) arrests digitoxin-induced (1.25 X10(-5) M--5 X 10(-5) M) fibrillation of individual cells and of cell groups. Veratramin alone and in combination with adrenalin does not change the inotropic parameters of individual cells. Bearing in mind that veratramin (2.5 X 10(-5) M) inhibits the accelerative effect of substances which activate and those which do not activate adenylatecyclse, as well as the fact that it has no effect on the content of cAMP in cultures and does not correct the increase in the cAMP content induced by adrenalin (5.5 X 10(-7) M), the authors conclude that the chronotropic effect of veratramin is not associated with the cell receptor mechanisms but is due to its effect on the transport of ions through the cell membrane, which is a responsible process in the generation of impulses."}
{"id": "PMID:470334", "title": "Adenylate cyclase in human gastric mucosa: its activation by histamine in morphologically different biopsy specimens.", "content": "In morphologically different biopsy specimens from fundic, antral and duodenal mucosa of 134 persons, basal and histamine stimulated adenylate cyclase activity was studied: Basal and stimulated adenylate cyclase activities were log-normally distributed. Only in the fundic but not in the antral and duodenal mucosa adenylate cyclase was sensitive to histamine. The mean basal activity in the fundic gastric mucosa was 148, in response to 10(-5) mol/l histamine 292 pmol cAMP/mg protein/20 min. In human fundic biopsy specimens histologically identified as normal gastric mucosa, the stimulatory effect of histamine on adenylate cyclase decreased with the individual's age. In bioptic material from patients suffering from histologically proven chronic gastritis the histamine effect decreased with the degree of atrophy. A similar loss of histamine sensitivity was found in gastric mucosal biopsies of antrectomized individuals operated at least 5 years before by the Billroth I or II method, whereas in the mucosa of patients with gastric or duodenal ulcer no loss occurred. In contrast, the most pronounced stimulatory action of histamine was found in this latter group. Since a histamine sensitive adenylate cyclase is localized only in the glandular area of the fundic mucosa and the histamine sensitivity depends on a morphological intact structure of the mucosa, it can be concluded, that the effects of histamine on adenylate cyclase and on hydrochloric acid acid secretion have to be considered as a mechanism linked together.", "contents": "Adenylate cyclase in human gastric mucosa: its activation by histamine in morphologically different biopsy specimens. In morphologically different biopsy specimens from fundic, antral and duodenal mucosa of 134 persons, basal and histamine stimulated adenylate cyclase activity was studied: Basal and stimulated adenylate cyclase activities were log-normally distributed. Only in the fundic but not in the antral and duodenal mucosa adenylate cyclase was sensitive to histamine. The mean basal activity in the fundic gastric mucosa was 148, in response to 10(-5) mol/l histamine 292 pmol cAMP/mg protein/20 min. In human fundic biopsy specimens histologically identified as normal gastric mucosa, the stimulatory effect of histamine on adenylate cyclase decreased with the individual's age. In bioptic material from patients suffering from histologically proven chronic gastritis the histamine effect decreased with the degree of atrophy. A similar loss of histamine sensitivity was found in gastric mucosal biopsies of antrectomized individuals operated at least 5 years before by the Billroth I or II method, whereas in the mucosa of patients with gastric or duodenal ulcer no loss occurred. In contrast, the most pronounced stimulatory action of histamine was found in this latter group. Since a histamine sensitive adenylate cyclase is localized only in the glandular area of the fundic mucosa and the histamine sensitivity depends on a morphological intact structure of the mucosa, it can be concluded, that the effects of histamine on adenylate cyclase and on hydrochloric acid acid secretion have to be considered as a mechanism linked together."}
{"id": "PMID:470335", "title": "[Acute combined alpha- and beta-adrenergic blockade in essential hypertension: effects on blood pressure, renal function, renin, and aldosterone].", "content": "In seven patients with uncomplicated essential hypertension the effects of an acute alpha-adrenergic blockade, alone and combined with a chronic beta-adrenergic blockade, on blood pressure, renal function as measured by standard clearance methods, plasma renin activity, and plasma aldosterone were evaluated. Acute alpha-adrenergic blockade with phentolamine (20 mg by intravenous infusion) significantly enhanced the antihypertensive effect of chronic beta-adrenergic blockade with slow-oxprenolol (160 mg/ day X 14 days) (- 14.5% verus - 7.4% for pulse pressure, - 12.4% versus - 6.0% for diastolic pressure, 2 alpha less than 0.05). Under combined adrenergic blockade renal plasma flow increased, glomerular filtration rate and filtration fraction decreased (2 alpha less than 0.05 each), whereas the fractional clearances of sodium, potassium, free water, and solute load remained unchanged. The activation of the renin-angiotensin-axis, elicited by alpha-adrenergic blockade alone, was suppressed by the preceding beta-adrenergic blockade. These findings demonstrate a favourable antihypertensive action of a combined blockade of alpha- und beta-adrenergic receptor sites without untoward side effects on renal function or the renin-angiotensin-axis.", "contents": "[Acute combined alpha- and beta-adrenergic blockade in essential hypertension: effects on blood pressure, renal function, renin, and aldosterone]. In seven patients with uncomplicated essential hypertension the effects of an acute alpha-adrenergic blockade, alone and combined with a chronic beta-adrenergic blockade, on blood pressure, renal function as measured by standard clearance methods, plasma renin activity, and plasma aldosterone were evaluated. Acute alpha-adrenergic blockade with phentolamine (20 mg by intravenous infusion) significantly enhanced the antihypertensive effect of chronic beta-adrenergic blockade with slow-oxprenolol (160 mg/ day X 14 days) (- 14.5% verus - 7.4% for pulse pressure, - 12.4% versus - 6.0% for diastolic pressure, 2 alpha less than 0.05). Under combined adrenergic blockade renal plasma flow increased, glomerular filtration rate and filtration fraction decreased (2 alpha less than 0.05 each), whereas the fractional clearances of sodium, potassium, free water, and solute load remained unchanged. The activation of the renin-angiotensin-axis, elicited by alpha-adrenergic blockade alone, was suppressed by the preceding beta-adrenergic blockade. These findings demonstrate a favourable antihypertensive action of a combined blockade of alpha- und beta-adrenergic receptor sites without untoward side effects on renal function or the renin-angiotensin-axis."}
{"id": "PMID:470336", "title": "[Unusually long survival times in patients with advanced Hodgkin's disease (author's transl)].", "content": "Of 18 patients with advanced Hodgkin's disease who obtained a complete remission with a modified De Vita treatment protocol 15 maintained their complete remission for an average of 76 months till the end of the observation time.", "contents": "[Unusually long survival times in patients with advanced Hodgkin's disease (author's transl)]. Of 18 patients with advanced Hodgkin's disease who obtained a complete remission with a modified De Vita treatment protocol 15 maintained their complete remission for an average of 76 months till the end of the observation time."}
{"id": "PMID:470339", "title": "The nexus in serial cross-sections of myocardium from cats subjected to hypovolemic shock. A three-dimensional interpretation.", "content": "Right ventricular papillary muscles from control cats and from cats subjected to hypovolemic shock were studied in cross-section, including serial sections, by electron microscopy. Loops of nexus and a redundant right angle nexus were followed through serial cross-sections in papillary muscles from the shock cats. These unusual nexus configurations were interpreted as a possible means of connecting perpendicular sheets of nexus, of interdigitating adjacent cells, and of providing slack to be taken up during the radial swelling of myocardial contraction. The flexibility of the intercalated disc which is implicit in this interpretation may explain the ability of cardiac myocytes to recover from the severe distortions adjacent to the intercalated disc which occur in myocardial zonal lesions, and perhaps, if they are reversible, in other lesions which occur in proximity to the intercalated disc.", "contents": "The nexus in serial cross-sections of myocardium from cats subjected to hypovolemic shock. A three-dimensional interpretation. Right ventricular papillary muscles from control cats and from cats subjected to hypovolemic shock were studied in cross-section, including serial sections, by electron microscopy. Loops of nexus and a redundant right angle nexus were followed through serial cross-sections in papillary muscles from the shock cats. These unusual nexus configurations were interpreted as a possible means of connecting perpendicular sheets of nexus, of interdigitating adjacent cells, and of providing slack to be taken up during the radial swelling of myocardial contraction. The flexibility of the intercalated disc which is implicit in this interpretation may explain the ability of cardiac myocytes to recover from the severe distortions adjacent to the intercalated disc which occur in myocardial zonal lesions, and perhaps, if they are reversible, in other lesions which occur in proximity to the intercalated disc."}
{"id": "PMID:470340", "title": "The effect of a liver protein synthesis inhibitor on plasma SAA levels in a model of accelerated amyloid deposition.", "content": "Animals treated with AgNO3 and amyloid-enhancing factor deposit large quantities of splenic amyloid in 48 hours. The kinetics of SAA production was examined in such a model in the presence and absence of an inhibitor of liver protein synthesis (ethionine). Ethionine had little effect on splenic protein synthesis but inhibited both the production of SAA and liver protein synthesis. When experiments were performed with fed mice, ethionine induced an inhibition of liver protein synthesis lasting 16 hours. Thereafter, liver protein synthesis began to recover, accompanied by a rise in plasma SAA levels. These results are consistent with hepatic but not splenic synthesis of SAA.", "contents": "The effect of a liver protein synthesis inhibitor on plasma SAA levels in a model of accelerated amyloid deposition. Animals treated with AgNO3 and amyloid-enhancing factor deposit large quantities of splenic amyloid in 48 hours. The kinetics of SAA production was examined in such a model in the presence and absence of an inhibitor of liver protein synthesis (ethionine). Ethionine had little effect on splenic protein synthesis but inhibited both the production of SAA and liver protein synthesis. When experiments were performed with fed mice, ethionine induced an inhibition of liver protein synthesis lasting 16 hours. Thereafter, liver protein synthesis began to recover, accompanied by a rise in plasma SAA levels. These results are consistent with hepatic but not splenic synthesis of SAA."}
{"id": "PMID:470345", "title": "Myointimal plaques in pulmonary vascular sclerosis associated with interstitial lung fibrosis.", "content": "In interstitial fibrosis of the lung, pulmonary vessels show priminent sclerotic changes. We have studied arteries in lung biopsies from patients with lung fibrosis of varied etiology using light and electron microscopy. It was found that the sclerotic change is essentially confined to arteries within fibrotic areas, areas of \"nonfibrotic\" lung having vessels with no intimal thickening. The changes are most striking in arteries with an external diameter of 500 micrometer. or greater (p less than 0.001), although smaller sized vessels also show significant changes. Ultrastructurally, the plaques are composed of typical smooth muscle cells with basement lamina, pinocytotic vesicles, dense bodies, and cytoplasmic microfilaments. These arterial changes could result from damage to the blood vessel which accompanies the mechanism producing lung fibrosis. Alternatively, the myointimal change could conceivably be part of an adaptive response following the establishment of fibrosis. In either instance, the narrowing would decrease blood flow to physiologically disadvantaged areas of the lung, and the muscle fibers might play an active role in reducing the blood flow.", "contents": "Myointimal plaques in pulmonary vascular sclerosis associated with interstitial lung fibrosis. In interstitial fibrosis of the lung, pulmonary vessels show priminent sclerotic changes. We have studied arteries in lung biopsies from patients with lung fibrosis of varied etiology using light and electron microscopy. It was found that the sclerotic change is essentially confined to arteries within fibrotic areas, areas of \"nonfibrotic\" lung having vessels with no intimal thickening. The changes are most striking in arteries with an external diameter of 500 micrometer. or greater (p less than 0.001), although smaller sized vessels also show significant changes. Ultrastructurally, the plaques are composed of typical smooth muscle cells with basement lamina, pinocytotic vesicles, dense bodies, and cytoplasmic microfilaments. These arterial changes could result from damage to the blood vessel which accompanies the mechanism producing lung fibrosis. Alternatively, the myointimal change could conceivably be part of an adaptive response following the establishment of fibrosis. In either instance, the narrowing would decrease blood flow to physiologically disadvantaged areas of the lung, and the muscle fibers might play an active role in reducing the blood flow."}
{"id": "PMID:470393", "title": "Cognitive structure and behavioral regulation in alcoholics.", "content": "Compared with nonalcoholics, alcoholics were less able to focus on relevant cues (i.e., they had weaker cognitive control) and less able to recall pertinent ideas or events and possible consequences (i.e., their internal scanning was narrower).", "contents": "Cognitive structure and behavioral regulation in alcoholics. Compared with nonalcoholics, alcoholics were less able to focus on relevant cues (i.e., they had weaker cognitive control) and less able to recall pertinent ideas or events and possible consequences (i.e., their internal scanning was narrower)."}
{"id": "PMID:470395", "title": "Identifying drinking problems in V.A. hospital patients.", "content": "Of 147 patients studied, clinicians identified 28 as problem drinkers; the patients' responses to interview items indicated as normal drinkers or abstainers, 9 indicated by interview responses that they were problem drinkers.", "contents": "Identifying drinking problems in V.A. hospital patients. Of 147 patients studied, clinicians identified 28 as problem drinkers; the patients' responses to interview items indicated as normal drinkers or abstainers, 9 indicated by interview responses that they were problem drinkers."}
{"id": "PMID:470397", "title": "College students' expectations of the results of drinking.", "content": "Social drinkers, when drinking, are expected to feel better and to be kinder, more fun to be with and more energetic and active than they would be if they were not drinking; inebriated alcoholics are expected to be meaner, less fun to be with and more lethargic than they would be if they were not inebriated.", "contents": "College students' expectations of the results of drinking. Social drinkers, when drinking, are expected to feel better and to be kinder, more fun to be with and more energetic and active than they would be if they were not drinking; inebriated alcoholics are expected to be meaner, less fun to be with and more lethargic than they would be if they were not inebriated."}
{"id": "PMID:470400", "title": "Age and BAC when arrested for drunken driving and public drunkenness.", "content": "Blood alcohol concentrations of young people arrested for driving while intoxicated and public drunkenness over a 4-year period in Randolph County, Arkansas, tended to be lower than those of older arrestees.", "contents": "Age and BAC when arrested for drunken driving and public drunkenness. Blood alcohol concentrations of young people arrested for driving while intoxicated and public drunkenness over a 4-year period in Randolph County, Arkansas, tended to be lower than those of older arrestees."}
{"id": "PMID:470401", "title": "Women A.A. members and nonalcoholics; scores on the Holmes and MacAndrew Scales of the MMPI.", "content": "Three groups of women-members of Alcoholics Anonymous, psychiatric inpatients and normal subjects--showed no significant differences in scores on the Holmes and MacAndrew Scales of the MMPI.", "contents": "Women A.A. members and nonalcoholics; scores on the Holmes and MacAndrew Scales of the MMPI. Three groups of women-members of Alcoholics Anonymous, psychiatric inpatients and normal subjects--showed no significant differences in scores on the Holmes and MacAndrew Scales of the MMPI."}
{"id": "PMID:470403", "title": "Men and women's responses to the Michigan Alcoholism Screening Test.", "content": "Men alcoholics scored significantly higher than women alcoholics on 7 of 24 items of the Michigan Alcoholism Screening Test; over-all, however, the test appears to be equally effective for both sexes.", "contents": "Men and women's responses to the Michigan Alcoholism Screening Test. Men alcoholics scored significantly higher than women alcoholics on 7 of 24 items of the Michigan Alcoholism Screening Test; over-all, however, the test appears to be equally effective for both sexes."}
{"id": "PMID:470404", "title": "Attitudes of paraprofessionals toward alcoholism; setting effects.", "content": "Paraprofessionals working in a residential alcoholism treatment program had significantly more \"custodial\" attitudes and attitudes of \"humanism\" toward alcoholics and beliefs concerning a psychological etiology of alcoholism than did paraprofessionals at a sobering-up station.", "contents": "Attitudes of paraprofessionals toward alcoholism; setting effects. Paraprofessionals working in a residential alcoholism treatment program had significantly more \"custodial\" attitudes and attitudes of \"humanism\" toward alcoholics and beliefs concerning a psychological etiology of alcoholism than did paraprofessionals at a sobering-up station."}
{"id": "PMID:470414", "title": "Ten years' experience with the Bj\u00f6rk-Shiley tilting disc valve.", "content": "More than 10 years of clinical experience with 1,800 consecutive valve replacements with the Bj\u00f6rk-Shiley tilting disc valve were focused on improving its durability, flow dynamics, thromboresistance, and in vivo functional control. Although the original Delrin disc functions excellently after 10 years in the human circulation, the durability of the prosthetic valve was further prolonged by the introduction of pyrolytic carbon as disc material. The opening mechanism was also reinforced by integrating the bearing struts with the valve ring. No other presently available heart valve prosthesis shows such a low resistance to flow for a given tissue diameter. The disc was equipped with a ring-shaped radiopaque marker that permitted noninvasive functional control. The 5 year survival rates were 82% after aortic, 66% after mitral, and 66% after mitral and aortic valve replacements, including the operative mortality rates. The incidence of systemic emboli was 0.7% per year after aortic, 4.2% per year after mitral, and 2.2% per year after mitral and aortic valve replacements with anticoagulation treatment. The incidence of obstruction by thrombosis was 0.3% per year for aortic, 1.3% per year for mitral, and 2.3% per year tricuspid prostheses. The new convex-concave model decreases the stagnation zone behind the disc, decreases emboli from 4.2% to 1.2% per year after mitral valve replacement, and has a lower gradient.", "contents": "Ten years' experience with the Bj\u00f6rk-Shiley tilting disc valve. More than 10 years of clinical experience with 1,800 consecutive valve replacements with the Bj\u00f6rk-Shiley tilting disc valve were focused on improving its durability, flow dynamics, thromboresistance, and in vivo functional control. Although the original Delrin disc functions excellently after 10 years in the human circulation, the durability of the prosthetic valve was further prolonged by the introduction of pyrolytic carbon as disc material. The opening mechanism was also reinforced by integrating the bearing struts with the valve ring. No other presently available heart valve prosthesis shows such a low resistance to flow for a given tissue diameter. The disc was equipped with a ring-shaped radiopaque marker that permitted noninvasive functional control. The 5 year survival rates were 82% after aortic, 66% after mitral, and 66% after mitral and aortic valve replacements, including the operative mortality rates. The incidence of systemic emboli was 0.7% per year after aortic, 4.2% per year after mitral, and 2.2% per year after mitral and aortic valve replacements with anticoagulation treatment. The incidence of obstruction by thrombosis was 0.3% per year for aortic, 1.3% per year for mitral, and 2.3% per year tricuspid prostheses. The new convex-concave model decreases the stagnation zone behind the disc, decreases emboli from 4.2% to 1.2% per year after mitral valve replacement, and has a lower gradient."}
{"id": "PMID:470416", "title": "Late failure of porcine valve heterografts in children.", "content": "Heterograft porcine valves have gained wide acceptance in replacement of diseased cardiac valves, and their clinical performance in adults has been very satisfactory over follow-up periods of up to 8 years. Valve replacement in children is relatively infrequent and experience with porcine xenografts is necessarily small. Our combined experience at three university hospitals has been with 25 children, 17 months to 16 years of age, who have been followed for 10 to 54 months (mean follow-up 33 months). Porcine valves were used to replace the aortic valve in nine, the mitral valve in seven, both valves in two, the tricuspid valve in two, and the pulmonary valve in five patients. Severe bioprosthetic valve dysfunction has occurred in five (20%) of these patients so far and necessitated replacement because of severe stenosis in mitral (two) or aortic (three) valve prostheses at 18 to 45 months after implantation; one postoperative death occurred among the five reoperations. Pathological examination showed extensive fragmentation of collagen with focal heavy calcification and degeneration. In addition we have encountered deterioration and calcification of two porcine valves in 23 valved conduits followed for 12 to 70 months (mean 43 months), requiring removal and replacement of the valves 65 and 67 months after implantation. This experience indicates a disquietingly high incidence of relatively early failure of porcine xenograft valves in children. This is significantly higher than the failure rate observed in adult patients. The failure rate is not consistently related to the small size of an implanted valve which becomes relatively narrow with the growth of the patient, leading to excessive turbulence and trauma to the prosthesis. Other factors, including increased turnover of calcium and accelerated rejection in growing children, may contribute to these failures and should be examined in order to improve long-term results. A satisfactory performance would make heterografts the ideal valvular prosthesis in children, since anticoagulation is avoided.", "contents": "Late failure of porcine valve heterografts in children. Heterograft porcine valves have gained wide acceptance in replacement of diseased cardiac valves, and their clinical performance in adults has been very satisfactory over follow-up periods of up to 8 years. Valve replacement in children is relatively infrequent and experience with porcine xenografts is necessarily small. Our combined experience at three university hospitals has been with 25 children, 17 months to 16 years of age, who have been followed for 10 to 54 months (mean follow-up 33 months). Porcine valves were used to replace the aortic valve in nine, the mitral valve in seven, both valves in two, the tricuspid valve in two, and the pulmonary valve in five patients. Severe bioprosthetic valve dysfunction has occurred in five (20%) of these patients so far and necessitated replacement because of severe stenosis in mitral (two) or aortic (three) valve prostheses at 18 to 45 months after implantation; one postoperative death occurred among the five reoperations. Pathological examination showed extensive fragmentation of collagen with focal heavy calcification and degeneration. In addition we have encountered deterioration and calcification of two porcine valves in 23 valved conduits followed for 12 to 70 months (mean 43 months), requiring removal and replacement of the valves 65 and 67 months after implantation. This experience indicates a disquietingly high incidence of relatively early failure of porcine xenograft valves in children. This is significantly higher than the failure rate observed in adult patients. The failure rate is not consistently related to the small size of an implanted valve which becomes relatively narrow with the growth of the patient, leading to excessive turbulence and trauma to the prosthesis. Other factors, including increased turnover of calcium and accelerated rejection in growing children, may contribute to these failures and should be examined in order to improve long-term results. A satisfactory performance would make heterografts the ideal valvular prosthesis in children, since anticoagulation is avoided."}
{"id": "PMID:470417", "title": "Operative treatment of aortic dissections. Experience with 125 patients over a sixteen-year period.", "content": "An unselected, consecutive cohort of 125 patients underwent operative repair of acute and chronic aortic dissections with tubular graft interposition over a 16 year span. The absence of remote geographical referral biases and the unselected nature of this series provided a patient population that was representative of the disease process (as assessed heretofore only from autopsy series). Furthermore, this enabled high-risk subsets to be defined by retrospective analysis. Patients were classified according to whether the ascending aorta was involved (type A with involvement, type B without), irrespective of the site of intimal tear, and according to age of the dissection: Fifty-three patients had acute type A (Ac-A), 29 had chronic type A (Ch-A), 20 had acute type B (Ac-B), and 23 had chronic type B (Ch-B) dissections. Fourteen percent (17/125) of the dissections had ruptured. Concomitant aortic valve replacement (AVR) was performed in 11% (6/53) for Ac-A cases and 38% (11/29) of the Ch-A cases. A total of 391 patient-years of follow-up was analyzed; follow-up averaged 4.5 years and extended to 13.7 years. Over-all operative mortality rate was 34% (18/53) for Ac-A, 14% (4/29) for Ch-A, 45% (9/20) for Ac-B, and 22% (5/23) for Ch-B; during the most recent 5 year interval these figures were lower: 27%, 8%, 20%, and 20%, respectively, N = 50. Multiple preoperative variables were found to correlate significantly with both operative death and long-term survival. Operative survivors generally experienced satisfactory functional benefit. Late attrition averaged 8% per year; 61% of all late deaths were related to cardiac or cerebral causes. Over-all actuarial survival (+/- SEM) for the entire cohort was 54% +/- 5% at 5 years and 26% +/- 7% at 10 years; for the 89 patients surviving operation, these figures were 76% +/- 5% and 37% +/- 10%, respectively. No significant differences in long-term survival were evident between the different subgroups. Whether the primary intimal tear had been resected or concomitant AVR had been performed had no statistically significant bearing on operative mortality, functional result, necessity for late reoperation, or late attrition. The long-term \"natural\" history of surgically treated patients with aortic dissections, as defined in this study, should facilitate comparison with other treatment modalities. Results of the present analysis support immediate operative intervention for patients with Ac-A dissections and probably for those with Ac-B dissections. Additionally, surgical treatment of patients with symptomatic or enlarging Ch-A and Ch-B dissections provides satisfactory rehabilitation and long-term survival. Finally, we re-emphasize our recommendation for simplified classification of aortic dissections, based solely upon the presence or absence of ascending aortic involvement. Pathophysiology and expected biologic behavior pivot on this feature, and appropriate clinical strategy can thereby be defined.", "contents": "Operative treatment of aortic dissections. Experience with 125 patients over a sixteen-year period. An unselected, consecutive cohort of 125 patients underwent operative repair of acute and chronic aortic dissections with tubular graft interposition over a 16 year span. The absence of remote geographical referral biases and the unselected nature of this series provided a patient population that was representative of the disease process (as assessed heretofore only from autopsy series). Furthermore, this enabled high-risk subsets to be defined by retrospective analysis. Patients were classified according to whether the ascending aorta was involved (type A with involvement, type B without), irrespective of the site of intimal tear, and according to age of the dissection: Fifty-three patients had acute type A (Ac-A), 29 had chronic type A (Ch-A), 20 had acute type B (Ac-B), and 23 had chronic type B (Ch-B) dissections. Fourteen percent (17/125) of the dissections had ruptured. Concomitant aortic valve replacement (AVR) was performed in 11% (6/53) for Ac-A cases and 38% (11/29) of the Ch-A cases. A total of 391 patient-years of follow-up was analyzed; follow-up averaged 4.5 years and extended to 13.7 years. Over-all operative mortality rate was 34% (18/53) for Ac-A, 14% (4/29) for Ch-A, 45% (9/20) for Ac-B, and 22% (5/23) for Ch-B; during the most recent 5 year interval these figures were lower: 27%, 8%, 20%, and 20%, respectively, N = 50. Multiple preoperative variables were found to correlate significantly with both operative death and long-term survival. Operative survivors generally experienced satisfactory functional benefit. Late attrition averaged 8% per year; 61% of all late deaths were related to cardiac or cerebral causes. Over-all actuarial survival (+/- SEM) for the entire cohort was 54% +/- 5% at 5 years and 26% +/- 7% at 10 years; for the 89 patients surviving operation, these figures were 76% +/- 5% and 37% +/- 10%, respectively. No significant differences in long-term survival were evident between the different subgroups. Whether the primary intimal tear had been resected or concomitant AVR had been performed had no statistically significant bearing on operative mortality, functional result, necessity for late reoperation, or late attrition. The long-term \"natural\" history of surgically treated patients with aortic dissections, as defined in this study, should facilitate comparison with other treatment modalities. Results of the present analysis support immediate operative intervention for patients with Ac-A dissections and probably for those with Ac-B dissections. Additionally, surgical treatment of patients with symptomatic or enlarging Ch-A and Ch-B dissections provides satisfactory rehabilitation and long-term survival. Finally, we re-emphasize our recommendation for simplified classification of aortic dissections, based solely upon the presence or absence of ascending aortic involvement. Pathophysiology and expected biologic behavior pivot on this feature, and appropriate clinical strategy can thereby be defined."}
{"id": "PMID:470418", "title": "Treatment of aneurysm of transverse aortic arch.", "content": "Although aneurysms involving the aortic arch are usually well localized and amenable to reconstructive operation, the reported results of this form of therapy at this level are not as good as in other portions of the aorta. The difference is due to cerebral and bleeding disturbances associated with cerebral protection techniques. This report describes 30 patients and emphasizes the variability of extent of these lesions and the results of methods employed for cerebral protection, which varied according to extent of disease. The aneurysm involved all but the proximal ascending aorta in one patient and was replaced with a permanent ascending aorta-innominate and left common carotid artery bypass graft. Eight aneurysms were limited to the transverse arch; one was removed with the aid of temporary bypass and seven with cardiopulmonary bypass and separate brachiocephalic normothermic perfusion. Temporary and permanent bypass grafts were used in four patients with lesser involvement. None of these techniques was used in 17 patients who had distal arch involvement. Of the 30 patients, 26 survived and 18 are still alive despite the treatment being spread over a 22 year period. Cerebral complications occurred in 3 patients, two of whom died. These problems were avoided in five patients treated more recently by using lows flows under low pressures. Coagulopathies did not occur. Although the technique of cardiopulmonary bypass, profound hypothermia, and circulatory arrest provides a more convenient and technically simpler method of operation, the disadvantages of coagulopathies with excessive bleeding, pulmonary problems, and lack of consistent cerebral protection argue against its routine use at this time.", "contents": "Treatment of aneurysm of transverse aortic arch. Although aneurysms involving the aortic arch are usually well localized and amenable to reconstructive operation, the reported results of this form of therapy at this level are not as good as in other portions of the aorta. The difference is due to cerebral and bleeding disturbances associated with cerebral protection techniques. This report describes 30 patients and emphasizes the variability of extent of these lesions and the results of methods employed for cerebral protection, which varied according to extent of disease. The aneurysm involved all but the proximal ascending aorta in one patient and was replaced with a permanent ascending aorta-innominate and left common carotid artery bypass graft. Eight aneurysms were limited to the transverse arch; one was removed with the aid of temporary bypass and seven with cardiopulmonary bypass and separate brachiocephalic normothermic perfusion. Temporary and permanent bypass grafts were used in four patients with lesser involvement. None of these techniques was used in 17 patients who had distal arch involvement. Of the 30 patients, 26 survived and 18 are still alive despite the treatment being spread over a 22 year period. Cerebral complications occurred in 3 patients, two of whom died. These problems were avoided in five patients treated more recently by using lows flows under low pressures. Coagulopathies did not occur. Although the technique of cardiopulmonary bypass, profound hypothermia, and circulatory arrest provides a more convenient and technically simpler method of operation, the disadvantages of coagulopathies with excessive bleeding, pulmonary problems, and lack of consistent cerebral protection argue against its routine use at this time."}
{"id": "PMID:470419", "title": "Patch enlargement of the aortic and mitral valve rings with aortic and mitral double valve replacement. Experimental study.", "content": "The experimental results of patch enlargement of the aortic and mitral valve rings with aortic and mitral double valve replacement are reported. The operative technique of this new surgical method is described and the indications are discussed.", "contents": "Patch enlargement of the aortic and mitral valve rings with aortic and mitral double valve replacement. Experimental study. The experimental results of patch enlargement of the aortic and mitral valve rings with aortic and mitral double valve replacement are reported. The operative technique of this new surgical method is described and the indications are discussed."}
{"id": "PMID:470420", "title": "Patch enlargement of the aortic valve ring by extending the aortic incision into the anterior mitral leaflet. New operative technique.", "content": "This communication describes a new surgical procedure of enlarging the narrow aortic valve ring by extending the aortic incision through the fibrous origin of the aortic leaflet of the mitral valve into this leaflet. A fusiform patch is sutured to the V-shaped defect in the aortic leaflet of the mitral valve and in the aortic anulus. This procedure permits the replacement of the aortic valve by a suitable prosthesis. Between June of 1976 and February of 1978, eight patients underwent this surgical procedure. At the time of operation the patients were between 8 and 50 years old. The estimated enlargement of the aortic root ranged from 10 to 25 mm. The operative technique is described, peculiarities of this method are discussed, and the results are reported. Six to 27 months following operation, the clinical condition of six patients is good. Four patients show no impairment of mitral valve function. In one case, preoperatively diagnosed mitral incompetence persists. In another patient the pericardial patch broke from the aortic leaflet of the mitral valve, so that the valve had to be replaced on the fourth postoperative day. One patient died of myocardial necrosis because of insufficient myocardial protection during operation. One child with acute aortic insufficiency caused by staphylococcal endocarditis and congestive heart failure died of septicemia 3 months postoperatively. Mitral incompetence was not detectable in this child.", "contents": "Patch enlargement of the aortic valve ring by extending the aortic incision into the anterior mitral leaflet. New operative technique. This communication describes a new surgical procedure of enlarging the narrow aortic valve ring by extending the aortic incision through the fibrous origin of the aortic leaflet of the mitral valve into this leaflet. A fusiform patch is sutured to the V-shaped defect in the aortic leaflet of the mitral valve and in the aortic anulus. This procedure permits the replacement of the aortic valve by a suitable prosthesis. Between June of 1976 and February of 1978, eight patients underwent this surgical procedure. At the time of operation the patients were between 8 and 50 years old. The estimated enlargement of the aortic root ranged from 10 to 25 mm. The operative technique is described, peculiarities of this method are discussed, and the results are reported. Six to 27 months following operation, the clinical condition of six patients is good. Four patients show no impairment of mitral valve function. In one case, preoperatively diagnosed mitral incompetence persists. In another patient the pericardial patch broke from the aortic leaflet of the mitral valve, so that the valve had to be replaced on the fourth postoperative day. One patient died of myocardial necrosis because of insufficient myocardial protection during operation. One child with acute aortic insufficiency caused by staphylococcal endocarditis and congestive heart failure died of septicemia 3 months postoperatively. Mitral incompetence was not detectable in this child."}
{"id": "PMID:470421", "title": "Anterior approach for tumor of the superior sulcus.", "content": "A new approach for the resection of tumors of the superior sulcus is described. The exposure is gained through a proximal median sternotomy extended into the anterior fourth intercostal space as well as to the base of the neck on the appropriate side. This approach guarantees excellent exposure of the tumor, subclavian artery, and brachial plexus as well as access to the ribs posteriorly and the border of the vertebral bodies. Hilar dissection is readily accomplished without change of the patient's position. Disadvantages relate to depth of exposure, especially in large individuals, and the complicated wound closure.", "contents": "Anterior approach for tumor of the superior sulcus. A new approach for the resection of tumors of the superior sulcus is described. The exposure is gained through a proximal median sternotomy extended into the anterior fourth intercostal space as well as to the base of the neck on the appropriate side. This approach guarantees excellent exposure of the tumor, subclavian artery, and brachial plexus as well as access to the ribs posteriorly and the border of the vertebral bodies. Hilar dissection is readily accomplished without change of the patient's position. Disadvantages relate to depth of exposure, especially in large individuals, and the complicated wound closure."}
{"id": "PMID:470422", "title": "Surgical treatment of Ebstein's anomaly. Early and late results in twenty patients subjected to valve replacement.", "content": "Twenty patients with Ebstein's anomaly underwent corrective operations between 1965 and 1978. Sixteen were in Functional Class III or IV (N.Y.H.A.) and four were in Class II but were having episodes of anoxia, syncope, or a rapid increase in cardiac size. In the first 10 cases (1965 to 1972) the tricuspid valve was replaced with a Starr-Edwards prosthesis, and in the second group (1972 to 1978) a dura mater valve was used. Five patients also underwent plication of a very large atrialized right ventricular segment. The atrial septal defect was closed totally in four cases, partially in eleven cases, and left open in five cases; one patient also underwent closure of the ventricular septal defect. Four patients died in the immediate postoperative period, three of whom were in the first group. There were three late postoperative deaths in the remaining 16 patients, all of them in the first group. All 13 of the surviving patients are in Functional Class I, except for one patient who is in Class II.", "contents": "Surgical treatment of Ebstein's anomaly. Early and late results in twenty patients subjected to valve replacement. Twenty patients with Ebstein's anomaly underwent corrective operations between 1965 and 1978. Sixteen were in Functional Class III or IV (N.Y.H.A.) and four were in Class II but were having episodes of anoxia, syncope, or a rapid increase in cardiac size. In the first 10 cases (1965 to 1972) the tricuspid valve was replaced with a Starr-Edwards prosthesis, and in the second group (1972 to 1978) a dura mater valve was used. Five patients also underwent plication of a very large atrialized right ventricular segment. The atrial septal defect was closed totally in four cases, partially in eleven cases, and left open in five cases; one patient also underwent closure of the ventricular septal defect. Four patients died in the immediate postoperative period, three of whom were in the first group. There were three late postoperative deaths in the remaining 16 patients, all of them in the first group. All 13 of the surviving patients are in Functional Class I, except for one patient who is in Class II."}
{"id": "PMID:470423", "title": "Septation of the univentricular heart. Transatrial approach.", "content": "The technique of septation of the univentricular heart via a transatrial approach is described in detail. Clinical details of four patients having Type A-III univentricular heart document the feasibility of working through the right-sided atrioventricular valve to place a cloth prosthesis into the ventricle to divide it into approximately equal chambers. Improved hemodynamics were observed in the postoperative period, which probably were related to retaining the integrity of the ventricular wall. This approach seems best suited to the ventricular chamber estimated to be large by echocardiography without severe pulmonary outflow tract obstruction. Techniques for enlargement of the pulmonary outflow tract are also described.", "contents": "Septation of the univentricular heart. Transatrial approach. The technique of septation of the univentricular heart via a transatrial approach is described in detail. Clinical details of four patients having Type A-III univentricular heart document the feasibility of working through the right-sided atrioventricular valve to place a cloth prosthesis into the ventricle to divide it into approximately equal chambers. Improved hemodynamics were observed in the postoperative period, which probably were related to retaining the integrity of the ventricular wall. This approach seems best suited to the ventricular chamber estimated to be large by echocardiography without severe pulmonary outflow tract obstruction. Techniques for enlargement of the pulmonary outflow tract are also described."}
{"id": "PMID:470424", "title": "Mustard's operation modified to avoid dysrhythmias and pulmonary and systemic venous obstruction.", "content": "Between, May of 1973 and May of 1978, 130 patients underwent correction of complete transposition of the great arteries by a modification of the Mustard operation specifically designed to avoid dysrhythmias and to minimize systemic and pulmonary venous obstruction. Of the 102 surviving patients who underwent this operation, 99 still exhibited sinus rhythm on the latest standard electrocardiographic (ECG) recording. On 24 hour ECG recording between 2 weeks and 4 years postoperatively, nine (15.5%) of 58 patients having the Mustard operation for simple and complex transposition showed dysrhythmias. There were no cases of pulmonary venous or inferior vena caval obstruction in this series. Six cases of superior vena caval obstruction were encountered. Our operative and autopsy findings point to the importance of avoiding the area of the sinus and atrioventricular nodes, since by doing so, we have reduced postoperative dysrhythmias. Operative technique, in particular, wide excision of the septum secundum and baffle design as suggested by Brom, have reduced the incidence of superior and inferior caval obstruction and pulmonary venous obstruction. The modifications we have applied in our series suggest that Mustard's operation should not be discarded prematurely.", "contents": "Mustard's operation modified to avoid dysrhythmias and pulmonary and systemic venous obstruction. Between, May of 1973 and May of 1978, 130 patients underwent correction of complete transposition of the great arteries by a modification of the Mustard operation specifically designed to avoid dysrhythmias and to minimize systemic and pulmonary venous obstruction. Of the 102 surviving patients who underwent this operation, 99 still exhibited sinus rhythm on the latest standard electrocardiographic (ECG) recording. On 24 hour ECG recording between 2 weeks and 4 years postoperatively, nine (15.5%) of 58 patients having the Mustard operation for simple and complex transposition showed dysrhythmias. There were no cases of pulmonary venous or inferior vena caval obstruction in this series. Six cases of superior vena caval obstruction were encountered. Our operative and autopsy findings point to the importance of avoiding the area of the sinus and atrioventricular nodes, since by doing so, we have reduced postoperative dysrhythmias. Operative technique, in particular, wide excision of the septum secundum and baffle design as suggested by Brom, have reduced the incidence of superior and inferior caval obstruction and pulmonary venous obstruction. The modifications we have applied in our series suggest that Mustard's operation should not be discarded prematurely."}
{"id": "PMID:470425", "title": "Growth of the pulmonary anulus and pulmonary arteries after the Waterston anastomosis.", "content": "Twenty-six patients with tetralogy of Fallot underwent angiocardiography before a Waterston shunt and again several years later, prior to complete repair (mean interval 40 +/- 15.6 [SD] months). The ratio between the diameter of the pulmonary valve anulus (PVA) and that of the descending thoracic aorta (Ao) increased by 0.32 +/- 0.233, significantly more than is reported to occur after a Blalock-Taussig anastomosis (p = 0.0001). This increase had some relation to the interval between the two angiographic studies (r = 0.34, p = 0.08) and to the mean pressure in the main pulmonary artery (MPA) at the time of the second study (r = 0.35, p = 0.08). The ratio of diameter of the right (RPA) and left pulmonary arteries (LPA) and that of the Ao also increased by 0.24 +/- 0.226 and 0.20 +/- 0.201, respectively. These data suggest that an initial large aortopulmonary shunt may result in less need for transannular patching at complete repair.", "contents": "Growth of the pulmonary anulus and pulmonary arteries after the Waterston anastomosis. Twenty-six patients with tetralogy of Fallot underwent angiocardiography before a Waterston shunt and again several years later, prior to complete repair (mean interval 40 +/- 15.6 [SD] months). The ratio between the diameter of the pulmonary valve anulus (PVA) and that of the descending thoracic aorta (Ao) increased by 0.32 +/- 0.233, significantly more than is reported to occur after a Blalock-Taussig anastomosis (p = 0.0001). This increase had some relation to the interval between the two angiographic studies (r = 0.34, p = 0.08) and to the mean pressure in the main pulmonary artery (MPA) at the time of the second study (r = 0.35, p = 0.08). The ratio of diameter of the right (RPA) and left pulmonary arteries (LPA) and that of the Ao also increased by 0.24 +/- 0.226 and 0.20 +/- 0.201, respectively. These data suggest that an initial large aortopulmonary shunt may result in less need for transannular patching at complete repair."}
{"id": "PMID:470426", "title": "Treatment of early postinfarction ventricular aneurysm by intra-aortic balloon pumping and surgery.", "content": "In nine patients with medically refractory left ventricular failure and/or ventricular arrhythmias, secondary to acute formation of a ventricular aneurysm, intra-aortic balloon pumping (IABP) was instituted 24 to 36 hours before diagnostic angiographic studies. Ventricular irritability was reduced and heart failure was controlled in all patients. Eight patients underwent operation, four within 3 weeks of an acute myocardial infarction and four within 3 months. All had resection of the recent infarction and two had myocardial revascularization as well. Two of the eight patients died in the early postoperative period from intractable ventricular fibrillation. All six patients who survived the operation (mean follow-up 12 months) had excellent clinical results. Ventricular irritability was suppressed and only one patient had residual heart failure. However, there was one late death 7 months after operation. The results suggest that surgical therapy may be effective in the management of medically unresponsive arrhythmias and/or congestive heart failure in the acute or intermediate postinfarction phase. IABP assistance was helpful in supporting the circulation and reducing ventricular irritability during the preoperative and postoperative periods.", "contents": "Treatment of early postinfarction ventricular aneurysm by intra-aortic balloon pumping and surgery. In nine patients with medically refractory left ventricular failure and/or ventricular arrhythmias, secondary to acute formation of a ventricular aneurysm, intra-aortic balloon pumping (IABP) was instituted 24 to 36 hours before diagnostic angiographic studies. Ventricular irritability was reduced and heart failure was controlled in all patients. Eight patients underwent operation, four within 3 weeks of an acute myocardial infarction and four within 3 months. All had resection of the recent infarction and two had myocardial revascularization as well. Two of the eight patients died in the early postoperative period from intractable ventricular fibrillation. All six patients who survived the operation (mean follow-up 12 months) had excellent clinical results. Ventricular irritability was suppressed and only one patient had residual heart failure. However, there was one late death 7 months after operation. The results suggest that surgical therapy may be effective in the management of medically unresponsive arrhythmias and/or congestive heart failure in the acute or intermediate postinfarction phase. IABP assistance was helpful in supporting the circulation and reducing ventricular irritability during the preoperative and postoperative periods."}
{"id": "PMID:470452", "title": "Total joint arthroplasty. The hip.", "content": "Total hip arthroplasty, one of the most successful orthopedic procedures of this century, can provide relief of pain and improved function in a wide variety of hip conditions. Long-term follow-up has shown, however, that problems can occur, especially with the femoral prosthesis. As a result of this, surface-replacement total hip arthroplasty was developed, and at present it appears to be a very useful procedure for younger patients with concentric hip disease. Both procedures are discussed along with restrictions and complications inherent in their use.", "contents": "Total joint arthroplasty. The hip. Total hip arthroplasty, one of the most successful orthopedic procedures of this century, can provide relief of pain and improved function in a wide variety of hip conditions. Long-term follow-up has shown, however, that problems can occur, especially with the femoral prosthesis. As a result of this, surface-replacement total hip arthroplasty was developed, and at present it appears to be a very useful procedure for younger patients with concentric hip disease. Both procedures are discussed along with restrictions and complications inherent in their use."}
{"id": "PMID:470453", "title": "Total joint arthroplasty. The knee.", "content": "Total knee replacement has become an established form of treatment for gonarthrosis and usually results in excellent relief of pain and approximately 90 degrees of joint motion with satisfactory joint stability. The anatomic stability that cannot be restored at surgery must be provided for by additional prosthetic stability. Fixation of prosthetic devices, particularly in the tibia, is marginal and results in an increased incidence of loosening when the quality of bone is weak, as in osteoporosis, or when shear stress is increased because of malalignment or prosthetic constraint. Resurfacing techniques provide the greatest options if surgical revision is necessary. Surgical goals should be realistically assessed so as to maintain the best potential for future treatment options.", "contents": "Total joint arthroplasty. The knee. Total knee replacement has become an established form of treatment for gonarthrosis and usually results in excellent relief of pain and approximately 90 degrees of joint motion with satisfactory joint stability. The anatomic stability that cannot be restored at surgery must be provided for by additional prosthetic stability. Fixation of prosthetic devices, particularly in the tibia, is marginal and results in an increased incidence of loosening when the quality of bone is weak, as in osteoporosis, or when shear stress is increased because of malalignment or prosthetic constraint. Resurfacing techniques provide the greatest options if surgical revision is necessary. Surgical goals should be realistically assessed so as to maintain the best potential for future treatment options."}
{"id": "PMID:470454", "title": "Total joint arthroplasty. The ankle.", "content": "When conservative measures fail to relieve significant disability due to ankle joint disease, the only surgical method of treatment has been arthrodesis. Because of less than ideal results and other long-term problems with ankle arthrodesis, and after numerous investigation to characterize the ankle mechanically, we developed a prosthetic ankle joint replacement. The Mayo total ankle replacement is a metal-on-polyethylene, congruent, constrained prosthesis. Analysis of 94 patients (102 ankle prostheses) revealed good clinical results in patients with rheumatoid arthritis and in older persons with posttraumatic degenerative disease. Younger, more active patients in the latter category had more disappointing results. Further design development is under way to improve range-of-motion characteristics, decrease constraint forces, and improve bone fixation of the prosthetic components.", "contents": "Total joint arthroplasty. The ankle. When conservative measures fail to relieve significant disability due to ankle joint disease, the only surgical method of treatment has been arthrodesis. Because of less than ideal results and other long-term problems with ankle arthrodesis, and after numerous investigation to characterize the ankle mechanically, we developed a prosthetic ankle joint replacement. The Mayo total ankle replacement is a metal-on-polyethylene, congruent, constrained prosthesis. Analysis of 94 patients (102 ankle prostheses) revealed good clinical results in patients with rheumatoid arthritis and in older persons with posttraumatic degenerative disease. Younger, more active patients in the latter category had more disappointing results. Further design development is under way to improve range-of-motion characteristics, decrease constraint forces, and improve bone fixation of the prosthetic components."}
{"id": "PMID:470455", "title": "Total joint arthroplasty. The foot.", "content": "A prosthesis has been designed with which to resurface the painful, degenerated first metatarsophalangeal joint. Clinical evaluation thus far has indicated satisfactory results in 16 of 18 instances. This procedure will probably give the best results in the older patient with hallux rigidus, but the length of follow-up is as yet too short to allow firm opinions to be formed.", "contents": "Total joint arthroplasty. The foot. A prosthesis has been designed with which to resurface the painful, degenerated first metatarsophalangeal joint. Clinical evaluation thus far has indicated satisfactory results in 16 of 18 instances. This procedure will probably give the best results in the older patient with hallux rigidus, but the length of follow-up is as yet too short to allow firm opinions to be formed."}
{"id": "PMID:470456", "title": "Total joint arthroplasty. Applications in children and adolescents.", "content": "Sixty total hip arthroplasties were performed in 47 severely disabled children and adolescents. Follow-up was from 1 to 5 years. The complication rate was 22%. Loss of the prosthesis occurred in only one patient, who had had multiple operations. Eighty-five percent of these patients had good to excellent results.", "contents": "Total joint arthroplasty. Applications in children and adolescents. Sixty total hip arthroplasties were performed in 47 severely disabled children and adolescents. Follow-up was from 1 to 5 years. The complication rate was 22%. Loss of the prosthesis occurred in only one patient, who had had multiple operations. Eighty-five percent of these patients had good to excellent results."}
{"id": "PMID:470457", "title": "Total joint arthroplasty. Applications in the management of bone tumors.", "content": "Limb-saving resection has become a valid alternative to amputation in carefully selected patients with bone tumors. As interest grows in this technique, efforts continue to improve our methods of bone and joint reconstruction to restore a functional extremity. Previously there was little interest in this form of treatment despite a limb-saving potential, because of inadequate methods of skeletal reconstruction. The purpose of this paper is to review these previous methods and to outline our present approach to the problem.", "contents": "Total joint arthroplasty. Applications in the management of bone tumors. Limb-saving resection has become a valid alternative to amputation in carefully selected patients with bone tumors. As interest grows in this technique, efforts continue to improve our methods of bone and joint reconstruction to restore a functional extremity. Previously there was little interest in this form of treatment despite a limb-saving potential, because of inadequate methods of skeletal reconstruction. The purpose of this paper is to review these previous methods and to outline our present approach to the problem."}
{"id": "PMID:470458", "title": "Total joint arthroplasty. The role of biomechanics.", "content": "The role of biomechanics is intricately involved in total joint replacement from its inception to its clinical use. By integrating knowledge of joint function and material properties, the artificial joint is designed and tested. After clinical use, the joint function is again studied and failure modes are defined. Biomechanics is the single most important discipline that can be employed to study the cause of failure in order to improve design and technique. This will increase the reliability of, and decrease the incidence of failure associated with, total joint replacement.", "contents": "Total joint arthroplasty. The role of biomechanics. The role of biomechanics is intricately involved in total joint replacement from its inception to its clinical use. By integrating knowledge of joint function and material properties, the artificial joint is designed and tested. After clinical use, the joint function is again studied and failure modes are defined. Biomechanics is the single most important discipline that can be employed to study the cause of failure in order to improve design and technique. This will increase the reliability of, and decrease the incidence of failure associated with, total joint replacement."}
{"id": "PMID:470459", "title": "Total joint arthroplasty: principles and guidelines for postoperative physiatric management.", "content": "Postoperative physiatric treatment is an integral part of the replacement of hips, knees, ankles, shoulders, elbows, wrists, and digital joints. Neuromuscular substitution patterns and incoordination usually prevail after joint replacement because of the usual long-term expectation and experience of pain, limitation of motion, fatigue, weakness, and the unavoidable operative trauma. The goals of postoperative physiatric treatment, in line with those of joint replacement surgery, are relief of pain and reestablishment of comfortable, normal neuromuscular functions and their reasonable, safe application in appropriate activities of daily living and of locomotion. By close collaboration between the Department of Orthopedic Surgery and that of Physical Medicine and Rehabilitation, certain principles and detailed protocols of postoperative management have been developed over the last 10 years. These are described in some detail for each of the joint replacements. Optimal results can be achieved only through meticulous attention to physical and psychosocial details, with close cooperation and communication among the involved services and persons.", "contents": "Total joint arthroplasty: principles and guidelines for postoperative physiatric management. Postoperative physiatric treatment is an integral part of the replacement of hips, knees, ankles, shoulders, elbows, wrists, and digital joints. Neuromuscular substitution patterns and incoordination usually prevail after joint replacement because of the usual long-term expectation and experience of pain, limitation of motion, fatigue, weakness, and the unavoidable operative trauma. The goals of postoperative physiatric treatment, in line with those of joint replacement surgery, are relief of pain and reestablishment of comfortable, normal neuromuscular functions and their reasonable, safe application in appropriate activities of daily living and of locomotion. By close collaboration between the Department of Orthopedic Surgery and that of Physical Medicine and Rehabilitation, certain principles and detailed protocols of postoperative management have been developed over the last 10 years. These are described in some detail for each of the joint replacements. Optimal results can be achieved only through meticulous attention to physical and psychosocial details, with close cooperation and communication among the involved services and persons."}
{"id": "PMID:470461", "title": "Chronic gouty nephropathy treated by long-term hemodialysis and allopurinol.", "content": "A patient with intractable tophaceous gout and advanced renal failure responded favorably to treatment by long-term hemodialysis and administration of allopurinol. Plasma uric acid levels returned to normal, tophi decreased in size, and the frequency of attacks of gout decreased markedly and permitted the patient to return to full-time employment.", "contents": "Chronic gouty nephropathy treated by long-term hemodialysis and allopurinol. A patient with intractable tophaceous gout and advanced renal failure responded favorably to treatment by long-term hemodialysis and administration of allopurinol. Plasma uric acid levels returned to normal, tophi decreased in size, and the frequency of attacks of gout decreased markedly and permitted the patient to return to full-time employment."}
{"id": "PMID:470466", "title": "Conservation of ribosomal fidelity during ageing.", "content": "The function of ribosomes prepared from the liver of young (2--5 months old) and senescent (15--26 months old) mice were compared in vitro. The conclusions that can be drawn from the liver of senescent mice is 10 to 40% lower than that of young mice; (2) the fidelity of translation does not change grossly with age; (3) the thermosensitivity of translational activity of ribosomes and the activity of ribosome-associated ribonuclease do not change with age; (4) there is an age-dependent accumulation of free 40S ribosomal subunits in the cytoplasm of mouse liver. The relation of age-dependent changes in the structure and function of ribosomes is discussed.", "contents": "Conservation of ribosomal fidelity during ageing. The function of ribosomes prepared from the liver of young (2--5 months old) and senescent (15--26 months old) mice were compared in vitro. The conclusions that can be drawn from the liver of senescent mice is 10 to 40% lower than that of young mice; (2) the fidelity of translation does not change grossly with age; (3) the thermosensitivity of translational activity of ribosomes and the activity of ribosome-associated ribonuclease do not change with age; (4) there is an age-dependent accumulation of free 40S ribosomal subunits in the cytoplasm of mouse liver. The relation of age-dependent changes in the structure and function of ribosomes is discussed."}
{"id": "PMID:470467", "title": "Fine structure of IMR-90 cells in culture as examined by scanning and transmission electron microscopy.", "content": "Cells from the new strain IMR-90 were examined by scanning (SEM) and transmission (TEM) electron microscopy at early, middle, and late population doubling levels. The cells are characteristically flattened and elongated and arranged in clusters from 1 to several cells thick. Long thin processes extend from the poles and sides of the cells. The number of blebs and microvilli on the cell surface varies. In later population doubling level (PDL) cultures, a larger number of cells have greater quantities of microvilli on their surface. It is suggested that the increased number of microvilli might represent an increased level of differentiation. By TEM the cells typically have elongated to oval shaped nuclei which are sometimes deeply invaginated. The cytoplasm contains a well developed Golgi region, elongated mitochondria, microtubules, filaments, a variety of vesicles, vacuoles and dense bodies and large amounts of RNA in the form of granular endoplasmic reticulum and ribosomes. Cytoplasmic appearance, particularly the number of dense bodies, varies widely at all PDL. With increasing PDL, cells tend to have nuclei with more condensed chromatin, and a cytoplasm containing less mitochondria and granular endoplasmic reticulum and more dense bodies. Also at later PDL there is a higher frequency of cells containing long, thin dense mitochondria as well as bizarre shaped mitochondria. In older populations there are many cells in a state of filamentous degeneration. Cells with large numbbers of surface projections (microvilli) tend to be correlated with an osmiophilic cytoplasm containing many filaments and numerous dense bodies.", "contents": "Fine structure of IMR-90 cells in culture as examined by scanning and transmission electron microscopy. Cells from the new strain IMR-90 were examined by scanning (SEM) and transmission (TEM) electron microscopy at early, middle, and late population doubling levels. The cells are characteristically flattened and elongated and arranged in clusters from 1 to several cells thick. Long thin processes extend from the poles and sides of the cells. The number of blebs and microvilli on the cell surface varies. In later population doubling level (PDL) cultures, a larger number of cells have greater quantities of microvilli on their surface. It is suggested that the increased number of microvilli might represent an increased level of differentiation. By TEM the cells typically have elongated to oval shaped nuclei which are sometimes deeply invaginated. The cytoplasm contains a well developed Golgi region, elongated mitochondria, microtubules, filaments, a variety of vesicles, vacuoles and dense bodies and large amounts of RNA in the form of granular endoplasmic reticulum and ribosomes. Cytoplasmic appearance, particularly the number of dense bodies, varies widely at all PDL. With increasing PDL, cells tend to have nuclei with more condensed chromatin, and a cytoplasm containing less mitochondria and granular endoplasmic reticulum and more dense bodies. Also at later PDL there is a higher frequency of cells containing long, thin dense mitochondria as well as bizarre shaped mitochondria. In older populations there are many cells in a state of filamentous degeneration. Cells with large numbbers of surface projections (microvilli) tend to be correlated with an osmiophilic cytoplasm containing many filaments and numerous dense bodies."}
{"id": "PMID:470468", "title": "The synthesis of glycosaminoglycans in aging rat liver. A brief note.", "content": "The synthesis of glycosaminoglycans (GAG) was studied in liver slices from postnatal (9 days), young (140 days), adult (490 days) and senescent (940 days) rats. It was found that the rate of synthesis was highest in postnatal rat liver and decreased to about half in young rats with no further reduction in adult and senescent age groups. The specific radioactivity of the precursors of GAG synthesis did not change with age. The synthesis pattern of specific types of GAG in postnatal liver was characterized by a significant higher percentage of chondroitin sulfate and hyaluronic acid. In the following age classes the profile of specific GAG synthesis did not change significantly (heparin sulfate: chondroitin sulfate\" hyaluronic acid: \"keratin sulfate\" = 84%:8.3%:1.5%:1.6%).", "contents": "The synthesis of glycosaminoglycans in aging rat liver. A brief note. The synthesis of glycosaminoglycans (GAG) was studied in liver slices from postnatal (9 days), young (140 days), adult (490 days) and senescent (940 days) rats. It was found that the rate of synthesis was highest in postnatal rat liver and decreased to about half in young rats with no further reduction in adult and senescent age groups. The specific radioactivity of the precursors of GAG synthesis did not change with age. The synthesis pattern of specific types of GAG in postnatal liver was characterized by a significant higher percentage of chondroitin sulfate and hyaluronic acid. In the following age classes the profile of specific GAG synthesis did not change significantly (heparin sulfate: chondroitin sulfate\" hyaluronic acid: \"keratin sulfate\" = 84%:8.3%:1.5%:1.6%)."}
{"id": "PMID:470469", "title": "Prediction of neonatal death or need for interhospital transfer by prenatal risk characteristics of mother.", "content": "A closed-circuit television system is implemented between neonatal specialists at a large neonatal care hospital and nurses caring for infants in the newborn nursery in a small community hospital located in an inner city, predominantly black, economically deprived area. Data are systematically collected relating prenatal risk characteristics of mothers to outcome measures, including selected intrapartum maternal complications, neonatal morbidity and mortality, neonatal therapeutic interventions, and transfer of neonates from the small hospital to the larger hospital. To our knowledge, study of factors predicting transfer has received little attention in the literature. A log linear model is used to identify specific groups of prenatal risk characteristics capable of predicting maternal and neonatal outcomes.", "contents": "Prediction of neonatal death or need for interhospital transfer by prenatal risk characteristics of mother. A closed-circuit television system is implemented between neonatal specialists at a large neonatal care hospital and nurses caring for infants in the newborn nursery in a small community hospital located in an inner city, predominantly black, economically deprived area. Data are systematically collected relating prenatal risk characteristics of mothers to outcome measures, including selected intrapartum maternal complications, neonatal morbidity and mortality, neonatal therapeutic interventions, and transfer of neonates from the small hospital to the larger hospital. To our knowledge, study of factors predicting transfer has received little attention in the literature. A log linear model is used to identify specific groups of prenatal risk characteristics capable of predicting maternal and neonatal outcomes."}
{"id": "PMID:470470", "title": "Assessing functional status among elderly patients: a comparison of questionnaire and service provider ratings.", "content": "This paper describes a comparative analysis of questionnaire-based measures of functional status and clinical ratings of disability made by general practitioners and health visitors. Both approaches to functional assessment were used in rating 92 elderly primary care patients in terms of their performance of 13 mobility and self-help activities. Simple dichotomous and more complex trichotomous measures of performance were used to summarize functional ability in both the questionnaire and the provider's evaluation. Agreement between questionnaire-based and rater assessments was greatest for less complex mobility and self-help functions in comparisons using both dichotomous and trichotomous scales.", "contents": "Assessing functional status among elderly patients: a comparison of questionnaire and service provider ratings. This paper describes a comparative analysis of questionnaire-based measures of functional status and clinical ratings of disability made by general practitioners and health visitors. Both approaches to functional assessment were used in rating 92 elderly primary care patients in terms of their performance of 13 mobility and self-help activities. Simple dichotomous and more complex trichotomous measures of performance were used to summarize functional ability in both the questionnaire and the provider's evaluation. Agreement between questionnaire-based and rater assessments was greatest for less complex mobility and self-help functions in comparisons using both dichotomous and trichotomous scales."}
{"id": "PMID:470471", "title": "Emergency medical service: delays, response time and survival.", "content": "A major concern in emergency medical services (EMS) planning has been the need to minimize ambulance response time, only one of several delays in reaching definitive care. Other important delays include patient delay in seeking medical care and dispatch delay. In this study, the importance of response time in light of other delays is discussed, and response time as one determinant of patient survival is analyzed, using Seattle's EMS system as a case study. The major conclusion is that paramedic response times influence short-term patient survival.", "contents": "Emergency medical service: delays, response time and survival. A major concern in emergency medical services (EMS) planning has been the need to minimize ambulance response time, only one of several delays in reaching definitive care. Other important delays include patient delay in seeking medical care and dispatch delay. In this study, the importance of response time in light of other delays is discussed, and response time as one determinant of patient survival is analyzed, using Seattle's EMS system as a case study. The major conclusion is that paramedic response times influence short-term patient survival."}
{"id": "PMID:470472", "title": "Implications of alternative sampling strategies for emergency medical service evaluation.", "content": "Evaluations of emergency medical service (EMS) programs have been ambiguous, due in part, to problems of sample definition. Four different sampling strategies were studied: 1) all patients in cardiac arrest; 2) patients with a final diagnosis of myocardial infarction (MI); 3) patients with an emergency room diagnosis of \"rule out MI\"; and 4) patients identified by the ambulance team as a possible MI. Using a regional data base of all ambulance runs, we created study samples based on each of these strategies and measured the error that may be introduced as a result of sample selection. Bias was measured along three parameters of EMS system performance: 1) observed incidence of MI in the ambulance system; 2) condition recognition--the ability of the ambulance team to correctly identify acute cardiac patients; and 3) emergency room and hospital mortality rates. The emergency room diagnosis strategy systematically excludes all false-positives, while samples based on the ambulance team's assessment omit all false-negatives. The final diagnosis strategy yields significant underestimates of cardiac mortality. Samples restricted to cardiac arrests result in biased estimates of both the incidence of MI and the number of deaths.", "contents": "Implications of alternative sampling strategies for emergency medical service evaluation. Evaluations of emergency medical service (EMS) programs have been ambiguous, due in part, to problems of sample definition. Four different sampling strategies were studied: 1) all patients in cardiac arrest; 2) patients with a final diagnosis of myocardial infarction (MI); 3) patients with an emergency room diagnosis of \"rule out MI\"; and 4) patients identified by the ambulance team as a possible MI. Using a regional data base of all ambulance runs, we created study samples based on each of these strategies and measured the error that may be introduced as a result of sample selection. Bias was measured along three parameters of EMS system performance: 1) observed incidence of MI in the ambulance system; 2) condition recognition--the ability of the ambulance team to correctly identify acute cardiac patients; and 3) emergency room and hospital mortality rates. The emergency room diagnosis strategy systematically excludes all false-positives, while samples based on the ambulance team's assessment omit all false-negatives. The final diagnosis strategy yields significant underestimates of cardiac mortality. Samples restricted to cardiac arrests result in biased estimates of both the incidence of MI and the number of deaths."}
{"id": "PMID:470473", "title": "Public challenge of physician authority.", "content": "A sample survey of the public in a midwestern state substantiates the existence of widespread challenges to the authority of physicians, a phenomenon previously reported only impressionistically in the media. Attitudes tending to reject physicians' right to direct their interaction with patients characterized more than half the sample and were related to younger age, higher educational level, and greater health knowledge, with a consumerist and anti-authority stance also explanatory. Actual challenging behavior occurred at least once for about half the group, but in this instance was related less to age and knowledge than to more extensive experience with the health care system, as well as a lack of trust in people in general and doctors' competence in particular. However, explained variance was modest, arguing that other variables, not identified in this study, are at work. Surprisingly, respondents' health status, race, sex, and pattern of insurance coverage had little impact on either attitude or behavior, while both knowledge and a general tendency to reject authority were influential factors. Implications for physician-patient relations in the future are discussed in light of a number of social changes, including the rising educational level of the American public.", "contents": "Public challenge of physician authority. A sample survey of the public in a midwestern state substantiates the existence of widespread challenges to the authority of physicians, a phenomenon previously reported only impressionistically in the media. Attitudes tending to reject physicians' right to direct their interaction with patients characterized more than half the sample and were related to younger age, higher educational level, and greater health knowledge, with a consumerist and anti-authority stance also explanatory. Actual challenging behavior occurred at least once for about half the group, but in this instance was related less to age and knowledge than to more extensive experience with the health care system, as well as a lack of trust in people in general and doctors' competence in particular. However, explained variance was modest, arguing that other variables, not identified in this study, are at work. Surprisingly, respondents' health status, race, sex, and pattern of insurance coverage had little impact on either attitude or behavior, while both knowledge and a general tendency to reject authority were influential factors. Implications for physician-patient relations in the future are discussed in light of a number of social changes, including the rising educational level of the American public."}
{"id": "PMID:470486", "title": "[Clinical course and recurrences of the nephrotic syndrome due to minimal histologic lesions. Review of 73 cases (author's transl)].", "content": "The clinical course of 73 patients with nephrotic syndrome due to minimal histologic lesions was studied. A renal biopsy was performed in all of the cases; 23 biopsies were studied by immunofluorescence and two renal biopsies were carried out in eight cases. The follow-up period varied from 6 months to 10 years. The following treatment were used: 23 patients received prednisone alone, 48 were given prednisone plus chlorambucil, and 2 were treated only with chlorambucil. Sixty-five patients achieved a complete remission; a total of 39 recurrences occurred in 23 of them. The relapses took place 16 months on the average following complete remission. Twenty-three patients (32.4 percent) cured with only prednisone. Forty-eight required the association with chlorambucil, 8 percent of whom experienced recurrences before being completed cured. Two individuals were treated with chlorambucil alone and ach;eved a complete and stable remission. Eight patients (10.95 percent) could not be cured. Five of them showed a glomerular focal hyalinosis in the second renal biopsy. Six patients had hematuria at the onset: two of them achieved complete remission with recurrences; four did not. Three of the latter had glomerular focal hyalinosis. There were small foci of immunoglobulin deposits in 13 cases; 11 of them obtained complete remission with relapses and 2 achieved partial remission.", "contents": "[Clinical course and recurrences of the nephrotic syndrome due to minimal histologic lesions. Review of 73 cases (author's transl)]. The clinical course of 73 patients with nephrotic syndrome due to minimal histologic lesions was studied. A renal biopsy was performed in all of the cases; 23 biopsies were studied by immunofluorescence and two renal biopsies were carried out in eight cases. The follow-up period varied from 6 months to 10 years. The following treatment were used: 23 patients received prednisone alone, 48 were given prednisone plus chlorambucil, and 2 were treated only with chlorambucil. Sixty-five patients achieved a complete remission; a total of 39 recurrences occurred in 23 of them. The relapses took place 16 months on the average following complete remission. Twenty-three patients (32.4 percent) cured with only prednisone. Forty-eight required the association with chlorambucil, 8 percent of whom experienced recurrences before being completed cured. Two individuals were treated with chlorambucil alone and ach;eved a complete and stable remission. Eight patients (10.95 percent) could not be cured. Five of them showed a glomerular focal hyalinosis in the second renal biopsy. Six patients had hematuria at the onset: two of them achieved complete remission with recurrences; four did not. Three of the latter had glomerular focal hyalinosis. There were small foci of immunoglobulin deposits in 13 cases; 11 of them obtained complete remission with relapses and 2 achieved partial remission."}
{"id": "PMID:470487", "title": "[Comparative functional study of 25 patients with intrathoracic sarcoidosis (author's transl)].", "content": "Pulmonary function in 25 patients with intrathoracic sarcoidosis was studied by three different methods: clinical, as related to the presence or absence of dispnea; response to corticoid treatment, and roentgenologic stage. A total of 60 examination were made on the 25 patients. Each examination included: pulmonary volume (vital capacity and RV/CT coefficient), ventilatory mechanics (elastance, resistance, critical flow, and specific conductance), and gas exchange (DCO/VA and (A-a) O2). The following results were obtained from statistical evaluation of the mean values compared with normal values: 1) patients with dyspnea showed a greater pulmonary elastance and (A-a) O2 than normal; 2) treatment with corticoids only produces an improvement in the diffusion capacity in respect to the total volume (DCO/VT); 3) examinations corresponding to the three different roentgenologic stages varied only in pulmonary volume and only when stage III was compared with stages I and II; 4) the terminal stage of the disease is physiologically similar to the typical pattern of emphysema.", "contents": "[Comparative functional study of 25 patients with intrathoracic sarcoidosis (author's transl)]. Pulmonary function in 25 patients with intrathoracic sarcoidosis was studied by three different methods: clinical, as related to the presence or absence of dispnea; response to corticoid treatment, and roentgenologic stage. A total of 60 examination were made on the 25 patients. Each examination included: pulmonary volume (vital capacity and RV/CT coefficient), ventilatory mechanics (elastance, resistance, critical flow, and specific conductance), and gas exchange (DCO/VA and (A-a) O2). The following results were obtained from statistical evaluation of the mean values compared with normal values: 1) patients with dyspnea showed a greater pulmonary elastance and (A-a) O2 than normal; 2) treatment with corticoids only produces an improvement in the diffusion capacity in respect to the total volume (DCO/VT); 3) examinations corresponding to the three different roentgenologic stages varied only in pulmonary volume and only when stage III was compared with stages I and II; 4) the terminal stage of the disease is physiologically similar to the typical pattern of emphysema."}
{"id": "PMID:470488", "title": "[Urinary excretion of hydroxyproline in patients with chronic respiratory insufficiency (author's transl)].", "content": "The level of urinary excretion of hydroxyproline is considered as an index of the metabolic activity of the collagen. It increases in situations which include an increase in the osteoblastic activity or in the bone resorption. In respiratory insufficiency a series of conditions occur which are theoretically capable of modyfing this parameter. Twelve patients (9 males and 3 women) with chronic respiratory disease in a situation of respiratory insufficiency (hypoxemia and/or hypercapnia at rest) were studied. The urinary excretion of hydroxyproline in these patients was 15.30 +/- 8.16 mg/day/m2, significantly greater than that of a control group with similar characteristics which was 9.97 +/- 3.07 mg/day/m2 (p less than 0.05; Student's t test). The existence of a significant correlation between the urinary excretion of hydroxyproline and the degree of hypoxemia (r = 0.66; p less than 0.01) was likewise verified; in the same way, although to a lesser degree with the hypercapnia (r = 0.62; p less than 0.05). The different factors capable of influencing the bone metabolism in respiratory insufficiency are discussed, as well as the effects of the medications used by these patients. It is possible, on the other hand, that the increase of the urinary excretion of hydroxyproline does not depend only on alterations in the metabolic condition of the bone, but also on a reduction in the hepatic metabolism of the amino acid in relation with gasometric modifications.", "contents": "[Urinary excretion of hydroxyproline in patients with chronic respiratory insufficiency (author's transl)]. The level of urinary excretion of hydroxyproline is considered as an index of the metabolic activity of the collagen. It increases in situations which include an increase in the osteoblastic activity or in the bone resorption. In respiratory insufficiency a series of conditions occur which are theoretically capable of modyfing this parameter. Twelve patients (9 males and 3 women) with chronic respiratory disease in a situation of respiratory insufficiency (hypoxemia and/or hypercapnia at rest) were studied. The urinary excretion of hydroxyproline in these patients was 15.30 +/- 8.16 mg/day/m2, significantly greater than that of a control group with similar characteristics which was 9.97 +/- 3.07 mg/day/m2 (p less than 0.05; Student's t test). The existence of a significant correlation between the urinary excretion of hydroxyproline and the degree of hypoxemia (r = 0.66; p less than 0.01) was likewise verified; in the same way, although to a lesser degree with the hypercapnia (r = 0.62; p less than 0.05). The different factors capable of influencing the bone metabolism in respiratory insufficiency are discussed, as well as the effects of the medications used by these patients. It is possible, on the other hand, that the increase of the urinary excretion of hydroxyproline does not depend only on alterations in the metabolic condition of the bone, but also on a reduction in the hepatic metabolism of the amino acid in relation with gasometric modifications."}
{"id": "PMID:470490", "title": "[Practical considerations in dealing with cerebral ischemia (author's transl)].", "content": "The handling of patients with cerebral ischemia is reviewed, taking into consideration recent concepts regarding etiopathogenesis along with new diagnostic and therapeutic methods. A particularly important new diagnostic method is computerized axial tomography. The subject is divided into four sections in order to present a practical outline. The first section deals with the arterial circulatory system. Evaluation of patients with arteriosclerosis of the vessels in the neck and/or intracranial are reviewed in some detail, according to whether the clinical manifestation was transitory ischemia, progressive cerebral infarction, or complete cerebral infarction. Emphasis is placed on the proper selection of diagnostic tests and application of therapy in each case. The second part is a discussion of the changes in arterial blood pressure in the etipathogenesis of stroke. Arterial hypertension is an important factor in production of small infarctions. In the third section a review is made of the role of the heart in transitory ischemia and as a cause of cerebral infarctions. Lastly, the hematologic factors which might contribute to the development of cerebral ischemia, along with the other causes, are mentioned.", "contents": "[Practical considerations in dealing with cerebral ischemia (author's transl)]. The handling of patients with cerebral ischemia is reviewed, taking into consideration recent concepts regarding etiopathogenesis along with new diagnostic and therapeutic methods. A particularly important new diagnostic method is computerized axial tomography. The subject is divided into four sections in order to present a practical outline. The first section deals with the arterial circulatory system. Evaluation of patients with arteriosclerosis of the vessels in the neck and/or intracranial are reviewed in some detail, according to whether the clinical manifestation was transitory ischemia, progressive cerebral infarction, or complete cerebral infarction. Emphasis is placed on the proper selection of diagnostic tests and application of therapy in each case. The second part is a discussion of the changes in arterial blood pressure in the etipathogenesis of stroke. Arterial hypertension is an important factor in production of small infarctions. In the third section a review is made of the role of the heart in transitory ischemia and as a cause of cerebral infarctions. Lastly, the hematologic factors which might contribute to the development of cerebral ischemia, along with the other causes, are mentioned."}
{"id": "PMID:470492", "title": "[Transitory ischemic accidents. A retrospective study of 150 cases of ischemic infarction in the region of the median cerebral artery (author's transl)].", "content": "Transitory ischemic accident is an episode that commences abruptly with subjective or objective neurological disturbances, persists for a brief time, and returns to normal within 24 hours of onset with complete recuperation of neurological function. Using this as a basic definition, the authors specify the characteristic symptoms of different types of transitory ischemic accident and review in retrospect 150 cases of ischemic infarction in the region of the median cerebral artery. Thirty-eight percent of the patients suffered transitory ischemic accidents prior to stroke. The symptoms included the following, in order of frequency: motor nerve disorder, sensory nerve disorders, speech disturbances, and visual defects. Most of these patients presented a definite stroke within less than one month's time following the last transitory ischemic accident. The similarity of the symptoms in both conditions was noticeable. The authors study the angiographic images, the pharmacologic and toxic previous histories, and other associated diseases in each patient. They point out, lastly, that transitory ischemic accident should be considered the first manifestation of a cerebrovascular disease and not just as an isolate, reversible episode of little importance.", "contents": "[Transitory ischemic accidents. A retrospective study of 150 cases of ischemic infarction in the region of the median cerebral artery (author's transl)]. Transitory ischemic accident is an episode that commences abruptly with subjective or objective neurological disturbances, persists for a brief time, and returns to normal within 24 hours of onset with complete recuperation of neurological function. Using this as a basic definition, the authors specify the characteristic symptoms of different types of transitory ischemic accident and review in retrospect 150 cases of ischemic infarction in the region of the median cerebral artery. Thirty-eight percent of the patients suffered transitory ischemic accidents prior to stroke. The symptoms included the following, in order of frequency: motor nerve disorder, sensory nerve disorders, speech disturbances, and visual defects. Most of these patients presented a definite stroke within less than one month's time following the last transitory ischemic accident. The similarity of the symptoms in both conditions was noticeable. The authors study the angiographic images, the pharmacologic and toxic previous histories, and other associated diseases in each patient. They point out, lastly, that transitory ischemic accident should be considered the first manifestation of a cerebrovascular disease and not just as an isolate, reversible episode of little importance."}
{"id": "PMID:470493", "title": "[Clinical protocol for study and treatment of bronchogenic carcinoma (author's transl)].", "content": "Bronchogenic carcinoma constitutes one of the primary causes of death in our population. The only means of controlling the disease in a significant way is by surgery, which about a third of the patients undergo. This fact, together with the age at which the pathology appears, its frequent association with chronic pulmonary disease, and the rapidity of its metastatic spread, make it necessary to establish a protocol for the study, diagnosis, and treatment of bronchogenic carcinoma. These norms should be applicable in prospective epidemiologic studies and for evaluating methods of diagnosis and treatment. The Interhospital Bronchogenic Carcinoma Co-operative Group has established a protocol, which includes the criteria of operability, resectability, preoperative examinations, indications of mediastinoscopy, etc. The authors mention the histopathologic classification, the TNM criteria, and the characteristics of localized or advanced disease.", "contents": "[Clinical protocol for study and treatment of bronchogenic carcinoma (author's transl)]. Bronchogenic carcinoma constitutes one of the primary causes of death in our population. The only means of controlling the disease in a significant way is by surgery, which about a third of the patients undergo. This fact, together with the age at which the pathology appears, its frequent association with chronic pulmonary disease, and the rapidity of its metastatic spread, make it necessary to establish a protocol for the study, diagnosis, and treatment of bronchogenic carcinoma. These norms should be applicable in prospective epidemiologic studies and for evaluating methods of diagnosis and treatment. The Interhospital Bronchogenic Carcinoma Co-operative Group has established a protocol, which includes the criteria of operability, resectability, preoperative examinations, indications of mediastinoscopy, etc. The authors mention the histopathologic classification, the TNM criteria, and the characteristics of localized or advanced disease."}
{"id": "PMID:470494", "title": "[Pulmonary infarction as a cause of pneumothorax: report of two cases (author's transl)].", "content": "Pulmonary infarction is a very uncommon cause of pneumothorax. The authors report two patients with pneumothorax arising as a complication of pulmonary infarction. One was a 72-year-old man who had hemoptysis, pleural effusion, and alveolar condensation. Four days later he developed a hydropneumothorax and pulmonary cavitation. He died of heart failure. The pulmonary infarction was not septic in this case. The other patient was a 12-year-old boy who suffered a septic embolism with cavitation as a result of an infected wound. He later developed a tension pneumothorax and died in a state of shock. The authors have found only 16 cases of pneumothorax as a complication of pulmonary infarction in the literature. It is surprising that, even though all infarctions are in contact with the pleural surface, the incidence of pneumothorax is not higher. The infarctions may or may not be septic. Cavitation is not necessarily present, though infarctions are usually cavitated before pneumothorax develops. Tension pneumothorax occurs in some cases.", "contents": "[Pulmonary infarction as a cause of pneumothorax: report of two cases (author's transl)]. Pulmonary infarction is a very uncommon cause of pneumothorax. The authors report two patients with pneumothorax arising as a complication of pulmonary infarction. One was a 72-year-old man who had hemoptysis, pleural effusion, and alveolar condensation. Four days later he developed a hydropneumothorax and pulmonary cavitation. He died of heart failure. The pulmonary infarction was not septic in this case. The other patient was a 12-year-old boy who suffered a septic embolism with cavitation as a result of an infected wound. He later developed a tension pneumothorax and died in a state of shock. The authors have found only 16 cases of pneumothorax as a complication of pulmonary infarction in the literature. It is surprising that, even though all infarctions are in contact with the pleural surface, the incidence of pneumothorax is not higher. The infarctions may or may not be septic. Cavitation is not necessarily present, though infarctions are usually cavitated before pneumothorax develops. Tension pneumothorax occurs in some cases."}
{"id": "PMID:470495", "title": "[Pulmonary tuberculosis in the outpatients clinics of the Social Security in Biscay (author's transl)].", "content": "The authors review the problem of active pulmonary tuberculosis in the clinics of the Social Security in Biscay. The high number of cases offers a wide panorama and the possibility of arriving at general conclusions within the Biscayan demographic structure. It is rather disturbing to discover an average of 24.37 cases per 100,000 inhabitants per year in this partial study of the situacion. The indexes for 1967 and 1974 were practically the same. Although the disease occurs with greatest frequency in persons between 30 and 60 years of age, there is a marked tendency for it to appear at increasingly lower ages. The disease is much more common in men than in women, and immigrates seem to be more susceptible than the native population. Pulmonary tuberculosis was reported as the cause of 1.12 percent of the 61,341 deaths registered in Biscay during the 8-year period under study (1967-1974). In view of this situation the authors insist on the urgent need for strategic methods to erradicate this serious menace to public health. They consider the role of tuberculosis sanatoriums in the state to be of outmost importance.", "contents": "[Pulmonary tuberculosis in the outpatients clinics of the Social Security in Biscay (author's transl)]. The authors review the problem of active pulmonary tuberculosis in the clinics of the Social Security in Biscay. The high number of cases offers a wide panorama and the possibility of arriving at general conclusions within the Biscayan demographic structure. It is rather disturbing to discover an average of 24.37 cases per 100,000 inhabitants per year in this partial study of the situacion. The indexes for 1967 and 1974 were practically the same. Although the disease occurs with greatest frequency in persons between 30 and 60 years of age, there is a marked tendency for it to appear at increasingly lower ages. The disease is much more common in men than in women, and immigrates seem to be more susceptible than the native population. Pulmonary tuberculosis was reported as the cause of 1.12 percent of the 61,341 deaths registered in Biscay during the 8-year period under study (1967-1974). In view of this situation the authors insist on the urgent need for strategic methods to erradicate this serious menace to public health. They consider the role of tuberculosis sanatoriums in the state to be of outmost importance."}
{"id": "PMID:470496", "title": "[Electrocardiographic disturbances in the left spontaneous pneumothorax. A case report (author's transl)].", "content": "Spontaneous pneumothorax is a relatively frequent acute medical problem. Acute chest pain, sudden dyspnea, and a sensation of discomfort are the usual clinical symptoms: these manifestations also occur in coronary occlusion, with which the condition my easily be misdiagnosed. This is especially true in cases of spontaneous pneumothorax of the left side because the ECG anomalies that arise could be erroneously confused with a coronary condition. The literature on the subject is reviewed and a case of left spontaneous pneumothorax is presented, pointing out the electrocardiographic disturbances: decrease of the amplitude of the QRS complex and R waves, inversion of the T wave in AVL and flattening of the T wave in most of the derivations, slight deviation of the electric axis of QRS toward the right, and phasic variation of voltage (very slight in this case). The importance of ECG studies in these cases is stressed in order to establish the differential diagnosis and avoid unnecessary delays in the application of the proper therapy.", "contents": "[Electrocardiographic disturbances in the left spontaneous pneumothorax. A case report (author's transl)]. Spontaneous pneumothorax is a relatively frequent acute medical problem. Acute chest pain, sudden dyspnea, and a sensation of discomfort are the usual clinical symptoms: these manifestations also occur in coronary occlusion, with which the condition my easily be misdiagnosed. This is especially true in cases of spontaneous pneumothorax of the left side because the ECG anomalies that arise could be erroneously confused with a coronary condition. The literature on the subject is reviewed and a case of left spontaneous pneumothorax is presented, pointing out the electrocardiographic disturbances: decrease of the amplitude of the QRS complex and R waves, inversion of the T wave in AVL and flattening of the T wave in most of the derivations, slight deviation of the electric axis of QRS toward the right, and phasic variation of voltage (very slight in this case). The importance of ECG studies in these cases is stressed in order to establish the differential diagnosis and avoid unnecessary delays in the application of the proper therapy."}
{"id": "PMID:470497", "title": "[Bile acids II. Physiopathologic and clinical aspects (author's transl)].", "content": "It is generally accepted that the bile acids are responsible for pathologies as a result of deficiency or by toxic action. Quantitative deficiency is difficult to evaluate but the normal pool of bile acids is generally considered to be between 2 and4 grams. Daily loss and replacement by synthesis is thought to be between 500 and 700 mg. There is experimental evidence to demonstrate the toxic action of certain bile acids on metabolic structures and processes. There is no doubt that alterations in the metabolism of bile acids give rise to certain pathologic aspects in some diseases of the gastrointestinal tract or the hepatobiliary system. There are other conditions, on the other hand, in which the study of these acids may reveal significant physiopathologic implications. The first group includes terminal ileopathy, blind loop syndrome, gastric ulcer, gastritis, cholestasis, cirrhosis of the liver, and cholelithiasis. In the second group are such diverse conditions as acute pancreatitis, cancer of the colon, endocrine disturbances, some hyperlipidemias, and others. Much of the present day understanding of the physiopathology of the bile acids will probably have to be revised in the nex few years, in view of the rapid advances being made in this field.", "contents": "[Bile acids II. Physiopathologic and clinical aspects (author's transl)]. It is generally accepted that the bile acids are responsible for pathologies as a result of deficiency or by toxic action. Quantitative deficiency is difficult to evaluate but the normal pool of bile acids is generally considered to be between 2 and4 grams. Daily loss and replacement by synthesis is thought to be between 500 and 700 mg. There is experimental evidence to demonstrate the toxic action of certain bile acids on metabolic structures and processes. There is no doubt that alterations in the metabolism of bile acids give rise to certain pathologic aspects in some diseases of the gastrointestinal tract or the hepatobiliary system. There are other conditions, on the other hand, in which the study of these acids may reveal significant physiopathologic implications. The first group includes terminal ileopathy, blind loop syndrome, gastric ulcer, gastritis, cholestasis, cirrhosis of the liver, and cholelithiasis. In the second group are such diverse conditions as acute pancreatitis, cancer of the colon, endocrine disturbances, some hyperlipidemias, and others. Much of the present day understanding of the physiopathology of the bile acids will probably have to be revised in the nex few years, in view of the rapid advances being made in this field."}
{"id": "PMID:470501", "title": "[Double tachycardia (hisian and auricular) in a bradycardia-tachycardia syndrome (author's transl)].", "content": "The present case corresponds to a patient with a mitro-aortic valvulopathy of rheumatic origin with syncopal episodes and nauseas, who, in the ECG showed alternatively an extreme sinusal bradycardia and supraventricular tachyarrhythmias. It was interpreted as a typical case of bradycardia-tachycardia syndrome in which the crises of tachyarrhythmias consisted of episodes of double tachycardia with incomplete supra-hisian atrial-ventricular dissociation, documented by obtaining right atrial electrograms and hisian-electrograms during the episodes. Treatment was initiated with verapamil (10 mg intravenously every 8 hours) after an endocavitary pacemaker of demand had been placed. After 3 months the result was very satisfactory with good tolerance to the medical treatment and complete disappearance of the symptomatology.", "contents": "[Double tachycardia (hisian and auricular) in a bradycardia-tachycardia syndrome (author's transl)]. The present case corresponds to a patient with a mitro-aortic valvulopathy of rheumatic origin with syncopal episodes and nauseas, who, in the ECG showed alternatively an extreme sinusal bradycardia and supraventricular tachyarrhythmias. It was interpreted as a typical case of bradycardia-tachycardia syndrome in which the crises of tachyarrhythmias consisted of episodes of double tachycardia with incomplete supra-hisian atrial-ventricular dissociation, documented by obtaining right atrial electrograms and hisian-electrograms during the episodes. Treatment was initiated with verapamil (10 mg intravenously every 8 hours) after an endocavitary pacemaker of demand had been placed. After 3 months the result was very satisfactory with good tolerance to the medical treatment and complete disappearance of the symptomatology."}
{"id": "PMID:470502", "title": "[Congenital intraduodenal diaphragm. Review of two new cases (author's transl)].", "content": "Two cases of congenital duodenal diaphragm were discovered during a review of 2.724 duodenal X-rays performed over the past 3 years. An analysis is made of the total of 40 cases published in the literature. The duodenal diaphragm is a thin wall located into the duodenal lumen with excentric perforation and which undergoes a progressive sacculation. Its embryologic development and close relationship to intraduodenal diverticulum are described. Clinical manifestations are few and unspecific, and the condition can easily be overlooked during surgery even when external palpation or digital exploration of the duodenal lumen is performed following duodenotomy. Special emphasis is placed on the significance of the X-ray images, though they are only orientative when dealing with this condition. Congenital duodenal diaphragm should be kept in mind as a possibility whenever chronic stenosis of the duodenum is diagnosed.", "contents": "[Congenital intraduodenal diaphragm. Review of two new cases (author's transl)]. Two cases of congenital duodenal diaphragm were discovered during a review of 2.724 duodenal X-rays performed over the past 3 years. An analysis is made of the total of 40 cases published in the literature. The duodenal diaphragm is a thin wall located into the duodenal lumen with excentric perforation and which undergoes a progressive sacculation. Its embryologic development and close relationship to intraduodenal diverticulum are described. Clinical manifestations are few and unspecific, and the condition can easily be overlooked during surgery even when external palpation or digital exploration of the duodenal lumen is performed following duodenotomy. Special emphasis is placed on the significance of the X-ray images, though they are only orientative when dealing with this condition. Congenital duodenal diaphragm should be kept in mind as a possibility whenever chronic stenosis of the duodenum is diagnosed."}
{"id": "PMID:470507", "title": "[Phantom tumor (author's transl)].", "content": "Phantom tumors are accumulations of pleural effusion in the interlobular spaces of the lungs. They appear in patients with heart failure simulating a lung tumor but disappearing with medical treatment. The encystment of the fluid is apparently due to congenital defects in the pleura, which tends to store up the transudate produced by heart failure. Another pathogenetic possibility is the existence of pleural adherences. The most common localization is in the minor cissure, perhaps because it is more easily identified in the posterior view of the chest X-ray. In most of these cases the pleural effusion is due to left heart failure, though pleural effusion in normally associated with right congestive heart failure. On the posterior chest X-ray the fluid is observed as a round or fusiform mass. Differential diagnosis should be established for measotheliomas, pulmonary infarctions, pulmonary or metastatic tumoral nodules, hydatic cysts, and tuberculomas. Sixteen cases of phantom tumors are reported; nine of them were localized on the minor cissure, five on the right major cissure, one on the left major cissure, and one case of double localization on the left major cissure and minor cissure. All of them were due to left congestive heart failure.", "contents": "[Phantom tumor (author's transl)]. Phantom tumors are accumulations of pleural effusion in the interlobular spaces of the lungs. They appear in patients with heart failure simulating a lung tumor but disappearing with medical treatment. The encystment of the fluid is apparently due to congenital defects in the pleura, which tends to store up the transudate produced by heart failure. Another pathogenetic possibility is the existence of pleural adherences. The most common localization is in the minor cissure, perhaps because it is more easily identified in the posterior view of the chest X-ray. In most of these cases the pleural effusion is due to left heart failure, though pleural effusion in normally associated with right congestive heart failure. On the posterior chest X-ray the fluid is observed as a round or fusiform mass. Differential diagnosis should be established for measotheliomas, pulmonary infarctions, pulmonary or metastatic tumoral nodules, hydatic cysts, and tuberculomas. Sixteen cases of phantom tumors are reported; nine of them were localized on the minor cissure, five on the right major cissure, one on the left major cissure, and one case of double localization on the left major cissure and minor cissure. All of them were due to left congestive heart failure."}
{"id": "PMID:470509", "title": "[Extraskeletal manifestations of unusual localization in a case of myeloma (author's transl)].", "content": "A 46-year-old woman presented and IgG myeloma without metastases at the time of the diagnosis. A complete hematologic remission was achieved with melphalan and prednisone, but the patient then developed a polysymptomatic condition with myelomatous metastases in different organs. In some cases these were confirmed histologically (meninges, stomach, skin, and scalp); in others there was a strong clinical evidence (liver, oral mucosa, lymph nodes, and lumbar vertebra). There were manifestations of the disease in all of these sites, while complete hematologic remission was maintained. The meningeal metastasis was treated by surgery and irradiation with orbital penetration; the lymph nodes were irradiated, and COPP polychemotherapy was given to treat the rest of the metastatic localizations. In this way an apparently remission was achieved temporarily. The incidence of each one of the extraskeletal manifestations in this unusual case of myeloma is reviewed in the literature.", "contents": "[Extraskeletal manifestations of unusual localization in a case of myeloma (author's transl)]. A 46-year-old woman presented and IgG myeloma without metastases at the time of the diagnosis. A complete hematologic remission was achieved with melphalan and prednisone, but the patient then developed a polysymptomatic condition with myelomatous metastases in different organs. In some cases these were confirmed histologically (meninges, stomach, skin, and scalp); in others there was a strong clinical evidence (liver, oral mucosa, lymph nodes, and lumbar vertebra). There were manifestations of the disease in all of these sites, while complete hematologic remission was maintained. The meningeal metastasis was treated by surgery and irradiation with orbital penetration; the lymph nodes were irradiated, and COPP polychemotherapy was given to treat the rest of the metastatic localizations. In this way an apparently remission was achieved temporarily. The incidence of each one of the extraskeletal manifestations in this unusual case of myeloma is reviewed in the literature."}
{"id": "PMID:470539", "title": "Bone scanning in otolaryngology.", "content": "Modern radionuclide bone scanning has introduced a new concept in physiologic and anatomic diagnostic imaging to general medicine. As otolaryngologists must diagnose and treat disease in relation to the bony and/or cartilaginous supporting structures of the neurocranium and upper airway, this modality should be included in the otolaryngologist's diagnostic armamentarium. It is the purpose of this manuscript to study the specific applications of bone scanning to our specialty at this time, based on clinical experience over the past three years. This thesis describes the development of bone scanning in general (history of nuclear medicine and nuclear physics; history of bone scanning in particular). General concepts in nuclear medicine are then presented; these include a discussion of nuclear semantics, principles of radioactive emmissions, the properties 99mTc as a radionuclide, and the tracer principle. On the basis of these general concepts, specific concepts in bone scanning are then brought forth. The physiology of bone and the action of the bone scan agents is presented. Further discussion considers the availability and production of the bone scan agent, patient factors, the gamma camera, the triphasic bone scan and the ultimate diagnostic principle of the bone scan. Clinical applications of bone scanning in otolaryngology are then presented in three sections. Proven areas of application include the evaluation of malignant tumors of the head and neck, the diagnosis of temporomandibular joint disorders, the diagnosis of facial fractures, the evaluation of osteomyelitis, nuclear medicine imaging of the larynx, and the assessment of systemic disease. Areas of adjunctive or supplementary value are also noted, such as diagnostic imaging of meningioma. Finally, areas of marginal value in the application of bone scanning are described.", "contents": "Bone scanning in otolaryngology. Modern radionuclide bone scanning has introduced a new concept in physiologic and anatomic diagnostic imaging to general medicine. As otolaryngologists must diagnose and treat disease in relation to the bony and/or cartilaginous supporting structures of the neurocranium and upper airway, this modality should be included in the otolaryngologist's diagnostic armamentarium. It is the purpose of this manuscript to study the specific applications of bone scanning to our specialty at this time, based on clinical experience over the past three years. This thesis describes the development of bone scanning in general (history of nuclear medicine and nuclear physics; history of bone scanning in particular). General concepts in nuclear medicine are then presented; these include a discussion of nuclear semantics, principles of radioactive emmissions, the properties 99mTc as a radionuclide, and the tracer principle. On the basis of these general concepts, specific concepts in bone scanning are then brought forth. The physiology of bone and the action of the bone scan agents is presented. Further discussion considers the availability and production of the bone scan agent, patient factors, the gamma camera, the triphasic bone scan and the ultimate diagnostic principle of the bone scan. Clinical applications of bone scanning in otolaryngology are then presented in three sections. Proven areas of application include the evaluation of malignant tumors of the head and neck, the diagnosis of temporomandibular joint disorders, the diagnosis of facial fractures, the evaluation of osteomyelitis, nuclear medicine imaging of the larynx, and the assessment of systemic disease. Areas of adjunctive or supplementary value are also noted, such as diagnostic imaging of meningioma. Finally, areas of marginal value in the application of bone scanning are described."}
{"id": "PMID:470595", "title": "[Effect of long-term moderately hard and hard physical work on the psychomotor efficiency and mental work capacity (II.)].", "content": "23 women, aged 19--24, of average physical efficiency, were examined. The following aspects were taken into account: an influence of long-lasting cyclic physical work on bicycle ergometer on the results obtained during the visual and auditory reaction time, the task performed with different speed using the test of visual and motorial coordination and Pi\u00f3rkowski's Test, as well as work efficiency in Pauli's test and learning and reproduction of nonsensical syllables. Long-lasting physical work resulted in a significant prolongation of the visual reaction time and increased number of mistakes in case of a task performed with maximum speed using the test of visual and motorial coordination. Negative but weak effects of physical effort were observed in the results of auditory reaction time and tasks checking the mental work efficiency. In the initial stage of the experiment (the early part) in some tasks a positive effect of physical effort was observed.", "contents": "[Effect of long-term moderately hard and hard physical work on the psychomotor efficiency and mental work capacity (II.)]. 23 women, aged 19--24, of average physical efficiency, were examined. The following aspects were taken into account: an influence of long-lasting cyclic physical work on bicycle ergometer on the results obtained during the visual and auditory reaction time, the task performed with different speed using the test of visual and motorial coordination and Pi\u00f3rkowski's Test, as well as work efficiency in Pauli's test and learning and reproduction of nonsensical syllables. Long-lasting physical work resulted in a significant prolongation of the visual reaction time and increased number of mistakes in case of a task performed with maximum speed using the test of visual and motorial coordination. Negative but weak effects of physical effort were observed in the results of auditory reaction time and tasks checking the mental work efficiency. In the initial stage of the experiment (the early part) in some tasks a positive effect of physical effort was observed."}
{"id": "PMID:470596", "title": "[Evaluation of acute toxic effect of 3-cyanopyridine].", "content": "General acute toxicity, irritating, and allergic effects of 3-cyanpyridine were studied. LD50 after oral administration amounts to 1185 mg/kg of body weight, 3-cyanpyridine is absorbed through the intact skin: administration of 3-cyanpyridine solution into rabbit skin caused death of animals. Histopathologic studies showed reversible liver and kidney injuries after single administration of lethal and sublethal doses. 3-cyanpyridine significantly irritates damaged skin and in a solid state produces an irreversible cornea opacity. Allergic effect was not found.", "contents": "[Evaluation of acute toxic effect of 3-cyanopyridine]. General acute toxicity, irritating, and allergic effects of 3-cyanpyridine were studied. LD50 after oral administration amounts to 1185 mg/kg of body weight, 3-cyanpyridine is absorbed through the intact skin: administration of 3-cyanpyridine solution into rabbit skin caused death of animals. Histopathologic studies showed reversible liver and kidney injuries after single administration of lethal and sublethal doses. 3-cyanpyridine significantly irritates damaged skin and in a solid state produces an irreversible cornea opacity. Allergic effect was not found."}
{"id": "PMID:470597", "title": "[Determination of phenol in the urine by means of gas chromatography].", "content": "A simple method of determination of phenol in urine was presented. The conditions of phenol combinations hydrolysis and extraction were the same as those used by Buchet (10). 2,4-dichlorophenol was used as an internal standard. The accuracy of this method for phenol concentrations in urine from 25 to 200 mg/l is +/- 8,2%. This method is specific both for the compounds excreted with physiological urine and the metabolites used in the industry of organic solvents.", "contents": "[Determination of phenol in the urine by means of gas chromatography]. A simple method of determination of phenol in urine was presented. The conditions of phenol combinations hydrolysis and extraction were the same as those used by Buchet (10). 2,4-dichlorophenol was used as an internal standard. The accuracy of this method for phenol concentrations in urine from 25 to 200 mg/l is +/- 8,2%. This method is specific both for the compounds excreted with physiological urine and the metabolites used in the industry of organic solvents."}
{"id": "PMID:470598", "title": "[Various disorders of cholesterol metabolism and their effect on the development of experimental arteriosclerosis in rats exposed to carbon disulfide].", "content": "Basing on the results of the author's own studies performed in a series of experiments in rats, the author discusses the mechanism of the atherosclerotic effect of CS2. The results of the studies carried out by the author indicated that the disturbances of body cholesterol metabolism (especially the increased rate of cholesterol synthesis and decreased rate of its degradation) contribute to elevation of cholesterol level in blood. The above alterations together with the cholesterol metabolism disturbances due to CS2 emerging directly in the aorta wall aggravate the development of the atherosclerotic changes in the vascular wall. The experimental (biochemical and morphological) evidences on the atherosclerotic effect of CS2 have been provided.", "contents": "[Various disorders of cholesterol metabolism and their effect on the development of experimental arteriosclerosis in rats exposed to carbon disulfide]. Basing on the results of the author's own studies performed in a series of experiments in rats, the author discusses the mechanism of the atherosclerotic effect of CS2. The results of the studies carried out by the author indicated that the disturbances of body cholesterol metabolism (especially the increased rate of cholesterol synthesis and decreased rate of its degradation) contribute to elevation of cholesterol level in blood. The above alterations together with the cholesterol metabolism disturbances due to CS2 emerging directly in the aorta wall aggravate the development of the atherosclerotic changes in the vascular wall. The experimental (biochemical and morphological) evidences on the atherosclerotic effect of CS2 have been provided."}
{"id": "PMID:470599", "title": "[Comparative evaluation of the functional state of the respiratory system in workers exposed to asbestos and cotton dust].", "content": "63 spinners exposed to cotton dust and 75 spinners exposed to asbestos dust were examined. In the women working in asbestos spinning room chronic nonspecific respiratory tract diseases prevalence was found to amount to 30% and in the cotton exposed group it was 15%. Mean values of lungs' vital capacity remained within the due values, although they were statistically significantly lower in asbestos exposed spinners (p less than 0,001). On the other hand, the percentage ratio of the forced expiratory capacity of 1 second was statistically significantly lower (0,001 less than p less than 0,01) in those exposed to cotton dust. The authors point to the occurrence of functional disturbances of the respiratory tract prior to radiological symptoms of lung asbestosis and their correlation with clinical symptoms.", "contents": "[Comparative evaluation of the functional state of the respiratory system in workers exposed to asbestos and cotton dust]. 63 spinners exposed to cotton dust and 75 spinners exposed to asbestos dust were examined. In the women working in asbestos spinning room chronic nonspecific respiratory tract diseases prevalence was found to amount to 30% and in the cotton exposed group it was 15%. Mean values of lungs' vital capacity remained within the due values, although they were statistically significantly lower in asbestos exposed spinners (p less than 0,001). On the other hand, the percentage ratio of the forced expiratory capacity of 1 second was statistically significantly lower (0,001 less than p less than 0,01) in those exposed to cotton dust. The authors point to the occurrence of functional disturbances of the respiratory tract prior to radiological symptoms of lung asbestosis and their correlation with clinical symptoms."}
{"id": "PMID:470600", "title": "Persistent traditional Yemenite ways of dealing with stress in Israel.", "content": "Using her own clinical-field case material, the author, an anthropologist working in ethnopsychiatry, analyzes and illustrates the contemporary Israeli-Yemenite cultural concepts and therapeutic approaches to mental illness. The sources and content of the traditional Yemenite health system are historically traced with particular emphasis on Judaism as the moral matrix. Despite the continuing identification and involvement with the traditional bases of health beliefs, it is pointed out that this folk conservatism has been no bar to the acceptance and utilization of scientific medicine. The Yemenite has been able to integrate, without apparent cognitive dissonance, both the traditional healers and the modern physicians. The moral approach of the Yemenite traditional healer in dealing with interpersonal, functional disorders, is reflected in the diagnostic and therapeutic behavioral and ritual prescriptions of Jewish religion and Islamic influences. In this connection, there is no incongruity perceived between the existence of the evil eye, devils and spirits possessing a person and the teachings of the Talmud.", "contents": "Persistent traditional Yemenite ways of dealing with stress in Israel. Using her own clinical-field case material, the author, an anthropologist working in ethnopsychiatry, analyzes and illustrates the contemporary Israeli-Yemenite cultural concepts and therapeutic approaches to mental illness. The sources and content of the traditional Yemenite health system are historically traced with particular emphasis on Judaism as the moral matrix. Despite the continuing identification and involvement with the traditional bases of health beliefs, it is pointed out that this folk conservatism has been no bar to the acceptance and utilization of scientific medicine. The Yemenite has been able to integrate, without apparent cognitive dissonance, both the traditional healers and the modern physicians. The moral approach of the Yemenite traditional healer in dealing with interpersonal, functional disorders, is reflected in the diagnostic and therapeutic behavioral and ritual prescriptions of Jewish religion and Islamic influences. In this connection, there is no incongruity perceived between the existence of the evil eye, devils and spirits possessing a person and the teachings of the Talmud."}
{"id": "PMID:470601", "title": "The effects of various degrees of social pressure.", "content": "If the social pressure exerted against a minority is overwhelming (slavery, concentration camps) no cultural efforts of the slaves or prisoners can be expected. Absence of any pressure is not conducive to high achievements either. A moderate degree of social pressure especially when combined with a possibility of social acceptance will stimulate minority people to perform well and to try to achieve status and acceptance. The cultural climate in which persons belonging to the minority grew up often determines their choice of endeavor in which to prove themselves and to achieve. Arthur Toynbee's thesis of the importance of Challenge and Response for the evolution of civilisations and Hans Selye's concept of Stress in biology are related to the proposed hypothesis. The author shows the usefulness of his hypothesis on examples from the history of Blacks in America and of the Jews.", "contents": "The effects of various degrees of social pressure. If the social pressure exerted against a minority is overwhelming (slavery, concentration camps) no cultural efforts of the slaves or prisoners can be expected. Absence of any pressure is not conducive to high achievements either. A moderate degree of social pressure especially when combined with a possibility of social acceptance will stimulate minority people to perform well and to try to achieve status and acceptance. The cultural climate in which persons belonging to the minority grew up often determines their choice of endeavor in which to prove themselves and to achieve. Arthur Toynbee's thesis of the importance of Challenge and Response for the evolution of civilisations and Hans Selye's concept of Stress in biology are related to the proposed hypothesis. The author shows the usefulness of his hypothesis on examples from the history of Blacks in America and of the Jews."}
{"id": "PMID:470602", "title": "A cultural anthropological approach to migrants' illness.", "content": "The impact of collective representations on a person's health may be considerable. It is worthwhile for those who work in the health services to get acquainted with the representations of their patients and with the world view in which they figure. This is illustrated by an example of a Moroccan in Holland and the application of local beliefs which caused a serious problem for attending doctors.", "contents": "A cultural anthropological approach to migrants' illness. The impact of collective representations on a person's health may be considerable. It is worthwhile for those who work in the health services to get acquainted with the representations of their patients and with the world view in which they figure. This is illustrated by an example of a Moroccan in Holland and the application of local beliefs which caused a serious problem for attending doctors."}
{"id": "PMID:470603", "title": "Developmental imperatives in the latter half of life: some clinical implications.", "content": "In the latter half of life men tend to shift from active to passive mastery, women the opposite. Four cases are described clinically and are discussed from the point of view of this thesis.", "contents": "Developmental imperatives in the latter half of life: some clinical implications. In the latter half of life men tend to shift from active to passive mastery, women the opposite. Four cases are described clinically and are discussed from the point of view of this thesis."}
{"id": "PMID:470604", "title": "The meaning attached: attitudes towards the mentally ill.", "content": "It was assumed that attitudes to the mentally ill differ among groups following a range of personal and social involvement, the four groups being patients, family members, professionals and non-involved persons. To test this assumption a semantic differential scale of qualities and constructs mental illness and questionnaire of situations on meeting the mentally ill were administered to 120 persons from these groups. Results indicate that a continuum of negative attitudes to the mentally ill exists, the most negative position being taken by the non-involved, the most positive by the professionals, with patients and family members in the middle. In response to the questionnaire it appears that close personal relations with the mentally ill and recent date of occurrence of disturbance are associated with positive reactions to the mentally ill.", "contents": "The meaning attached: attitudes towards the mentally ill. It was assumed that attitudes to the mentally ill differ among groups following a range of personal and social involvement, the four groups being patients, family members, professionals and non-involved persons. To test this assumption a semantic differential scale of qualities and constructs mental illness and questionnaire of situations on meeting the mentally ill were administered to 120 persons from these groups. Results indicate that a continuum of negative attitudes to the mentally ill exists, the most negative position being taken by the non-involved, the most positive by the professionals, with patients and family members in the middle. In response to the questionnaire it appears that close personal relations with the mentally ill and recent date of occurrence of disturbance are associated with positive reactions to the mentally ill."}
{"id": "PMID:470605", "title": "Social profitableness: pathway to community acceptance of psychiatric patients.", "content": "The concept of 'social profitableness' is explicated as a crucial determinant in community acceptance of formerly hospitalized psychiatric patients. Increase emphasis upon patients' capability to provide rewards to citizens as well as to forgo costly deviant behavior is recommended.", "contents": "Social profitableness: pathway to community acceptance of psychiatric patients. The concept of 'social profitableness' is explicated as a crucial determinant in community acceptance of formerly hospitalized psychiatric patients. Increase emphasis upon patients' capability to provide rewards to citizens as well as to forgo costly deviant behavior is recommended."}
{"id": "PMID:470606", "title": "Linguistic aspects of transformative labelling: the case of repentant delinquents.", "content": "This article describes the linguistic changes that occurred amongst a group of repentant delinquents and assesses their importance in the self-reconstitution process. Each of the three major themes of transformative labelling are not only reflected in the way that the repentants talk; they are also highly influenced by it. In particular, the adoption of new pattern of speech helps the repentants to (i) come to terms with their delinquent past and turn over a new leaf and (ii) forestall the doubts that are bound to occur during the escalation to normal identity.", "contents": "Linguistic aspects of transformative labelling: the case of repentant delinquents. This article describes the linguistic changes that occurred amongst a group of repentant delinquents and assesses their importance in the self-reconstitution process. Each of the three major themes of transformative labelling are not only reflected in the way that the repentants talk; they are also highly influenced by it. In particular, the adoption of new pattern of speech helps the repentants to (i) come to terms with their delinquent past and turn over a new leaf and (ii) forestall the doubts that are bound to occur during the escalation to normal identity."}
{"id": "PMID:470607", "title": "Teachers as caregivers: mental health consultation in an army base.", "content": "This article recounts the application of mental health consultation techniques to a special army framework. Specifically, female soldiers serving as teachers for a problematic population of male recruits, received group consultation from a psychologist serving in the reserves. By training the teachers as therapeutic agents or caregivers for the soldiers, it was hoped to achieve greater effectiveness in their work, and to reach individually as many soldiers as possible. These soldiers were taking part in a special enrichment and training program organized by Zahal (Israel Defense Forces) for those whose illiteracy or emotional difficulties had exempted them in the past from regular army service. The consultation process and subjects raised at group sessions are discussed.", "contents": "Teachers as caregivers: mental health consultation in an army base. This article recounts the application of mental health consultation techniques to a special army framework. Specifically, female soldiers serving as teachers for a problematic population of male recruits, received group consultation from a psychologist serving in the reserves. By training the teachers as therapeutic agents or caregivers for the soldiers, it was hoped to achieve greater effectiveness in their work, and to reach individually as many soldiers as possible. These soldiers were taking part in a special enrichment and training program organized by Zahal (Israel Defense Forces) for those whose illiteracy or emotional difficulties had exempted them in the past from regular army service. The consultation process and subjects raised at group sessions are discussed."}
{"id": "PMID:470608", "title": "A token economy in the framework of a hospital therapeutic community -- practice and psychosocial implications.", "content": "A token economy (TE) aimed at enhancing self care, work habits, and social participation was initiated in conjunction with the restructuring of a chronic ward into a therapeutic community. Recorded data over a year revealed a differential impact of the TE on various patients. An attempt is made to characterize differential response modes to the TE and to delineate their correlates. Both participants in the program and therapeutic agents were interviewed as to their attitudes towards the TE. Beneficial effects were demonstrated mainly in patients with a relatively late onset of psychiatric illness, but a favorable attitude towards the TE was displayed by both patients and staff members. Implications for psychosocial readaptation are discussed taking into account humanistic and psychodynamic points of view. The reconsideration of possible merging of different therapeutic techniques seems to be desirable.", "contents": "A token economy in the framework of a hospital therapeutic community -- practice and psychosocial implications. A token economy (TE) aimed at enhancing self care, work habits, and social participation was initiated in conjunction with the restructuring of a chronic ward into a therapeutic community. Recorded data over a year revealed a differential impact of the TE on various patients. An attempt is made to characterize differential response modes to the TE and to delineate their correlates. Both participants in the program and therapeutic agents were interviewed as to their attitudes towards the TE. Beneficial effects were demonstrated mainly in patients with a relatively late onset of psychiatric illness, but a favorable attitude towards the TE was displayed by both patients and staff members. Implications for psychosocial readaptation are discussed taking into account humanistic and psychodynamic points of view. The reconsideration of possible merging of different therapeutic techniques seems to be desirable."}
{"id": "PMID:470609", "title": "Attitudes of physicians in a general hospital towards psychiatric consultation service.", "content": "The attitudes of 77 physicians in a general university hospital towards the psychiatric consultation service were studied using a questionnaire. The main findings were that the physicians found the psychiatric consultation helpful and not too difficult to obtain. Many of them wanted regular participation of the psychiatrist in the routine of their departments and in case conferences. The findings indicate that the expectations of the departments of surgery are for a consultation service and those of medicine for liaison service. The discrepancy between the positive attitudes of the physicians and the dissatisfaction of the consultants strongly suggest that emotional problems of the consultant, i.e. ambivalence about their profession, is the source of the common tension between psychiatric consultant and other physicians.", "contents": "Attitudes of physicians in a general hospital towards psychiatric consultation service. The attitudes of 77 physicians in a general university hospital towards the psychiatric consultation service were studied using a questionnaire. The main findings were that the physicians found the psychiatric consultation helpful and not too difficult to obtain. Many of them wanted regular participation of the psychiatrist in the routine of their departments and in case conferences. The findings indicate that the expectations of the departments of surgery are for a consultation service and those of medicine for liaison service. The discrepancy between the positive attitudes of the physicians and the dissatisfaction of the consultants strongly suggest that emotional problems of the consultant, i.e. ambivalence about their profession, is the source of the common tension between psychiatric consultant and other physicians."}
{"id": "PMID:470610", "title": "Remarks on the communication of psychotic children as seen in group therapy.", "content": "A group of 26 psychotic children, aged 5--8, in a day hospital, was treated in small groups of 4 or 5, by 2 therapists (a male and female). According to our observations the treatment set-up enhances a 'present therapeutic symbiosis' where the 'other half' of the symbiotic dyad is composed of all the children in the group, the therapists and the physical environment. The newly acquired imitation (echolalia and echopraxia) impraxia) improves the child's means of expression and is used for communication. It also helps the therapists in their task of decodifying the messages to the children and themselves.", "contents": "Remarks on the communication of psychotic children as seen in group therapy. A group of 26 psychotic children, aged 5--8, in a day hospital, was treated in small groups of 4 or 5, by 2 therapists (a male and female). According to our observations the treatment set-up enhances a 'present therapeutic symbiosis' where the 'other half' of the symbiotic dyad is composed of all the children in the group, the therapists and the physical environment. The newly acquired imitation (echolalia and echopraxia) impraxia) improves the child's means of expression and is used for communication. It also helps the therapists in their task of decodifying the messages to the children and themselves."}
{"id": "PMID:470611", "title": "Murder on the job: case report of a unique disaster.", "content": "This paper reports the experience of two psychiatrists who intervened in the aftermath of a 'disaster' affecting a small company of 75 people. The incident causing the crisis was a shooting spree in which an employee, responding to fantasied conspiratorial behavior among co-workers, shot and killed three of them, wounded two others and then killed himself. The company sanctioned and paid for this intervention and subsequently used the psychiatrists for formal and informal consultations. The report notes the similarities to and the differences from other reports for disasters in the literature. The clear link between present and past was noted in the emotional struggles of the survivors, and the value of helping them to establish these connections. The intervention appeared to be useful to the individuals and the company.", "contents": "Murder on the job: case report of a unique disaster. This paper reports the experience of two psychiatrists who intervened in the aftermath of a 'disaster' affecting a small company of 75 people. The incident causing the crisis was a shooting spree in which an employee, responding to fantasied conspiratorial behavior among co-workers, shot and killed three of them, wounded two others and then killed himself. The company sanctioned and paid for this intervention and subsequently used the psychiatrists for formal and informal consultations. The report notes the similarities to and the differences from other reports for disasters in the literature. The clear link between present and past was noted in the emotional struggles of the survivors, and the value of helping them to establish these connections. The intervention appeared to be useful to the individuals and the company."}
{"id": "PMID:470612", "title": "Calibration for quantitative X-ray microanalysis of skeletal muscle cells in culture.", "content": "Skeletal muscle in culture is used to demonstrate that the intracellular concentration of elements in tissue culture cells, especially of diffusible ions such as sodium, chlorine, potassium, and calcium can be measured by X-ray microanalysis in the scanning electron microscope. Quantitative analysis is possible by comparison with X-ray spectra of cells incubated in electrolyte solutions of known concentration. The minimum detectable concentration is approximately 2 mM.", "contents": "Calibration for quantitative X-ray microanalysis of skeletal muscle cells in culture. Skeletal muscle in culture is used to demonstrate that the intracellular concentration of elements in tissue culture cells, especially of diffusible ions such as sodium, chlorine, potassium, and calcium can be measured by X-ray microanalysis in the scanning electron microscope. Quantitative analysis is possible by comparison with X-ray spectra of cells incubated in electrolyte solutions of known concentration. The minimum detectable concentration is approximately 2 mM."}
{"id": "PMID:470613", "title": "Studies on conformational changes in F-actin of glycerinated muscle fibers during relaxation by means of polarized ultraviolet fluorescence microscopy.", "content": "By means of polarized ultraviolet fluorescence microscopy the conformational changes of F-actin occuring in glycerinated muscle fibers of rabbit and barnacle (Balanus rostratus Hock.) under the influence of adenosine triphosphate in the presence of ethylene glycol bis(beta-amino-ethyl ether)-N,N'-tetraacetic acid were discovered. These changes seem to be located near the surface of the globules thus hampering the penetration of univalent iones and neutral molecules into the F-actin macromolecule. It is suggested that similar changes of F-actin take place in thin myofilaments of living muscle fiber during the contraction-relaxation process.", "contents": "Studies on conformational changes in F-actin of glycerinated muscle fibers during relaxation by means of polarized ultraviolet fluorescence microscopy. By means of polarized ultraviolet fluorescence microscopy the conformational changes of F-actin occuring in glycerinated muscle fibers of rabbit and barnacle (Balanus rostratus Hock.) under the influence of adenosine triphosphate in the presence of ethylene glycol bis(beta-amino-ethyl ether)-N,N'-tetraacetic acid were discovered. These changes seem to be located near the surface of the globules thus hampering the penetration of univalent iones and neutral molecules into the F-actin macromolecule. It is suggested that similar changes of F-actin take place in thin myofilaments of living muscle fiber during the contraction-relaxation process."}
{"id": "PMID:470625", "title": "[Alpha-ketoglutarate dehydrogenase activity of Candida lipolytica during alpha-ketoglutarate biosynthesis].", "content": "The activity of alpha-ketoglutarate dehydrogenase (KGDH) was assayed in cell-free yeast homogenates with respect to studying the mechanism of alpha-ketoglutarate (KG) overproduction by the thiamine-heterotrophic culture of Candida lipolytica in media with hexadecane. Two types of yeast cells were used for analysis: (1) cells grown in the conditions of thiamine excess (500 mcg/l) and taken at the logariphmic growth phase; (2) cells grown in the conditions of thiamine deficiency and taken at the beginning of KG overproduction when the cultural growth was limited by thiamine. The activity of KGDH was assayed in the absence of thiamine diphosphate (TDP) and in its presence, the content of the holoenzyme and the total content of the holoenzyme and the apoenzyme being determined respectively. The activity of holoKGDH in the thiamine-deficient cells of C. lipolytica producing KG was almost five times lower than in the cells grown in the conditions of thiamine excess. In the latter, all KGDH was in the form of the holoenzyme; in the thiamine-deficient cells, 90% of KDGH was in the form of the apoenzyme devoid of TDP and therefore lacking the enzyme activity. The total content of holoKGDH IN THE THIAMINE-DEFICIENt cells producing KG was twice as high as in the cells grown in the conditions of thiamine excess. The data obtained are discussed in relation with the mechanism of KG biosynthesis from n-alkanes by yeast cells.", "contents": "[Alpha-ketoglutarate dehydrogenase activity of Candida lipolytica during alpha-ketoglutarate biosynthesis]. The activity of alpha-ketoglutarate dehydrogenase (KGDH) was assayed in cell-free yeast homogenates with respect to studying the mechanism of alpha-ketoglutarate (KG) overproduction by the thiamine-heterotrophic culture of Candida lipolytica in media with hexadecane. Two types of yeast cells were used for analysis: (1) cells grown in the conditions of thiamine excess (500 mcg/l) and taken at the logariphmic growth phase; (2) cells grown in the conditions of thiamine deficiency and taken at the beginning of KG overproduction when the cultural growth was limited by thiamine. The activity of KGDH was assayed in the absence of thiamine diphosphate (TDP) and in its presence, the content of the holoenzyme and the total content of the holoenzyme and the apoenzyme being determined respectively. The activity of holoKGDH in the thiamine-deficient cells of C. lipolytica producing KG was almost five times lower than in the cells grown in the conditions of thiamine excess. In the latter, all KGDH was in the form of the holoenzyme; in the thiamine-deficient cells, 90% of KDGH was in the form of the apoenzyme devoid of TDP and therefore lacking the enzyme activity. The total content of holoKGDH IN THE THIAMINE-DEFICIENt cells producing KG was twice as high as in the cells grown in the conditions of thiamine excess. The data obtained are discussed in relation with the mechanism of KG biosynthesis from n-alkanes by yeast cells."}
{"id": "PMID:470627", "title": "[Biomass accumulation by polyploid forms of Candida guilliermondii yeasts].", "content": "Under the action of a mitotic poison (acenaphthene), the haploid culture of Candida guilliermondii assimilating n-alkanes yielded a polyploid form whose cells were four times larger, on the average, than in the haploid culture. When the pure culture was grown under stationary conditions in media with glucose, the economical coefficient, i. e. the ratio between the assimilated glucose and the accumulated biomass, was by 16, 47 and 157% higher in the polyploid culture than in the haploid one after 24, 48 and 72 hours, respectively. The amount of biomass and the content of protein in it were the same in the haploid and diploid cultures grown in liquid nutrient media with n-alkanes. Cells of the polyploid culture were always larger in media containing n-alkanes, which facilitated separation; no cells were found after it in the cultural fluid. Not all cells of the haploid culture could be separated; therefore, the polyploid culture had the advantage over it. The polyploid cultures of C. guilliermondii were very stable when stored in the lyophilized state. Their morphological and physiological properties did not change within two years.", "contents": "[Biomass accumulation by polyploid forms of Candida guilliermondii yeasts]. Under the action of a mitotic poison (acenaphthene), the haploid culture of Candida guilliermondii assimilating n-alkanes yielded a polyploid form whose cells were four times larger, on the average, than in the haploid culture. When the pure culture was grown under stationary conditions in media with glucose, the economical coefficient, i. e. the ratio between the assimilated glucose and the accumulated biomass, was by 16, 47 and 157% higher in the polyploid culture than in the haploid one after 24, 48 and 72 hours, respectively. The amount of biomass and the content of protein in it were the same in the haploid and diploid cultures grown in liquid nutrient media with n-alkanes. Cells of the polyploid culture were always larger in media containing n-alkanes, which facilitated separation; no cells were found after it in the cultural fluid. Not all cells of the haploid culture could be separated; therefore, the polyploid culture had the advantage over it. The polyploid cultures of C. guilliermondii were very stable when stored in the lyophilized state. Their morphological and physiological properties did not change within two years."}
{"id": "PMID:470628", "title": "[Exopolysaccharide and proteolytic enzyme synthesis by M-, S- and R-forms of Mycobacterium lacticolum].", "content": "The composition of exocellular polysaccharides was studied in the M, S and R variants of Mycobacterium lacticolum 104. The exoglycans of the M and S variants were found to be rather similar but differ considerably from the exoglycan of the R form. The polysaccharides of the M and S cells contained glucose, mannose, galactose, and pyruvic acid; the exoglycan of the R cells contained also the fourth sugar arabinose, but lacked pyruvic acid. The specific rotation of the polysaccharides from the M, S and R variants ([alpha]d20), respectively, was +207.5, +168.0 and +25.0. The caseinolytic activity of the M, S and R variants, respectively, by the seventh day of growth was 0.93+/-0.21, 0.62+/-0.07 and 0.47+/-0.07 units/ml.", "contents": "[Exopolysaccharide and proteolytic enzyme synthesis by M-, S- and R-forms of Mycobacterium lacticolum]. The composition of exocellular polysaccharides was studied in the M, S and R variants of Mycobacterium lacticolum 104. The exoglycans of the M and S variants were found to be rather similar but differ considerably from the exoglycan of the R form. The polysaccharides of the M and S cells contained glucose, mannose, galactose, and pyruvic acid; the exoglycan of the R cells contained also the fourth sugar arabinose, but lacked pyruvic acid. The specific rotation of the polysaccharides from the M, S and R variants ([alpha]d20), respectively, was +207.5, +168.0 and +25.0. The caseinolytic activity of the M, S and R variants, respectively, by the seventh day of growth was 0.93+/-0.21, 0.62+/-0.07 and 0.47+/-0.07 units/ml."}
{"id": "PMID:470626", "title": "[Efficiency of glucose utilization by Gluconobacter oxydans].", "content": "The dynamics of growth and acid production in Gluconobacter oxydans cultures at various glucose concentrations has been investigated. Dinitrophenol (10-4 M) was shown to have effect on hexonic acids formation by the growing culture and resting cells of G. oxydans, as well as on the values of Y0. G. oxydans molar growth yield for glucose have been calculated. Oxidative transformation of glucose was shown to be not involved in energy supply of processes connected with the reproduction of G. oxydans. Glucose concentration in the growth medium determines the efficiency of utilization of this carbon and energy source.", "contents": "[Efficiency of glucose utilization by Gluconobacter oxydans]. The dynamics of growth and acid production in Gluconobacter oxydans cultures at various glucose concentrations has been investigated. Dinitrophenol (10-4 M) was shown to have effect on hexonic acids formation by the growing culture and resting cells of G. oxydans, as well as on the values of Y0. G. oxydans molar growth yield for glucose have been calculated. Oxidative transformation of glucose was shown to be not involved in energy supply of processes connected with the reproduction of G. oxydans. Glucose concentration in the growth medium determines the efficiency of utilization of this carbon and energy source."}
{"id": "PMID:470631", "title": "[Induced variability of the lipase producing fungus Rhizopus microsporus].", "content": "The most effective way for inducing mutants of Rhizopus microsporus with an elevated lipolytic activity is the combined action of nitrosomethyl urea and UV. Variants with the lipolytic activity of 3600--3700 units per 1 ml have been produced, thus being by 72--75% more effective than the parent culture.", "contents": "[Induced variability of the lipase producing fungus Rhizopus microsporus]. The most effective way for inducing mutants of Rhizopus microsporus with an elevated lipolytic activity is the combined action of nitrosomethyl urea and UV. Variants with the lipolytic activity of 3600--3700 units per 1 ml have been produced, thus being by 72--75% more effective than the parent culture."}
{"id": "PMID:470632", "title": "[Dynamics of the development of different microorganisms in soil].", "content": "The dynamics of individual components from the complex of soil microorganisms (bacteria, fungi, actinomycetes) and the population of Stm. olivocinereus introduced into soil was studied by luminescent microscopy and inoculation. The population density maxima for individual components from the complex of soil microorganisms were shown to be separated in time suggesting a succession. Fungi developed at the first steps of succession and dominated in biomass over the remaining components of the complex. Bacteria and actinomycetes developed at the later steps of succession. Glucose addition stimulated still earlier growth of fungi (the maximal mycelium legth was registered by the second day) but had hardly any effect on the dynamics of other components of the complex.", "contents": "[Dynamics of the development of different microorganisms in soil]. The dynamics of individual components from the complex of soil microorganisms (bacteria, fungi, actinomycetes) and the population of Stm. olivocinereus introduced into soil was studied by luminescent microscopy and inoculation. The population density maxima for individual components from the complex of soil microorganisms were shown to be separated in time suggesting a succession. Fungi developed at the first steps of succession and dominated in biomass over the remaining components of the complex. Bacteria and actinomycetes developed at the later steps of succession. Glucose addition stimulated still earlier growth of fungi (the maximal mycelium legth was registered by the second day) but had hardly any effect on the dynamics of other components of the complex."}
{"id": "PMID:470629", "title": "[Cyst formation by methanosarcina].", "content": "The morphology of a coccoid, methane producing bacterium growing on acetate was studied. The organism is capable of forming morphologically differentiated cells, nicrocysts, whose structure resembles that of bacterial surviving cells. The organism from peculiar macrocysts in the enrichment culture. In its other characteristics, the organism is similar to Methanococcus mazei. However, it clasibied as Methanosarcina, biotype 3, due to the characteristic formation of multicellular pseudococci.", "contents": "[Cyst formation by methanosarcina]. The morphology of a coccoid, methane producing bacterium growing on acetate was studied. The organism is capable of forming morphologically differentiated cells, nicrocysts, whose structure resembles that of bacterial surviving cells. The organism from peculiar macrocysts in the enrichment culture. In its other characteristics, the organism is similar to Methanococcus mazei. However, it clasibied as Methanosarcina, biotype 3, due to the characteristic formation of multicellular pseudococci."}
{"id": "PMID:470633", "title": "[Nitrogen fixing activity of pea nodule bacteria during different phases of host plant development].", "content": "The activity of nitrogenase in nodules of pea plants and the rate of diurnal nitrogen fixation were studied at different phases of the plant growth using the acetylene and isotope techniques. At the same time, the morphology of nodule bacteria was studied by electron and phase-contrast microscopy. About 90% of the bacteria in nodules were found in the form of bacteroids from the early phase of the plant growth (budding) to ripening when gemmated arthrospores could be observed on some bacteroids. The highest activity of nitrogenase and nitrogen fixation was detected at the reproductive stage of growth. Therefore, in order to obtain more active strains of pea nodule bacteria, they should be isolated from nodules at this stage of plant growth.", "contents": "[Nitrogen fixing activity of pea nodule bacteria during different phases of host plant development]. The activity of nitrogenase in nodules of pea plants and the rate of diurnal nitrogen fixation were studied at different phases of the plant growth using the acetylene and isotope techniques. At the same time, the morphology of nodule bacteria was studied by electron and phase-contrast microscopy. About 90% of the bacteria in nodules were found in the form of bacteroids from the early phase of the plant growth (budding) to ripening when gemmated arthrospores could be observed on some bacteroids. The highest activity of nitrogenase and nitrogen fixation was detected at the reproductive stage of growth. Therefore, in order to obtain more active strains of pea nodule bacteria, they should be isolated from nodules at this stage of plant growth."}
{"id": "PMID:470630", "title": "[Effect of ionizing radiation on the structural and ultrastructural organization of Mycobacterium rubrum].", "content": "The structural and ultrastructural organization of Mycobacterium rubrum cells was studied in the course of normal growth and in the conditions of irradiation. The cytomorphological differentiation of the bacterial membrane structure was shown as well as their functional role. Ionizing radiation was used as a tool for revealing the role of the membrane structures; the latter were found to be related to a certain cellular function. Damages of various, individually functioning membrane systems upon the action of ionizing radiation on the cell are described. Mesosomes are absent from the cells if the synthesis of peptidoglycan is inhibited, whereas hypersynthesis of the cell wall material is observed upon their hypertrophy. The content of mitochondrial analogs in the cell increases with the rate of metabolic processes. The function of cellular division is disturbed as a result of damages in the nucleoid DNA structure and degradation of nucleoidosomes. Changes in carbohydrate and lipid metabolism are also observed.", "contents": "[Effect of ionizing radiation on the structural and ultrastructural organization of Mycobacterium rubrum]. The structural and ultrastructural organization of Mycobacterium rubrum cells was studied in the course of normal growth and in the conditions of irradiation. The cytomorphological differentiation of the bacterial membrane structure was shown as well as their functional role. Ionizing radiation was used as a tool for revealing the role of the membrane structures; the latter were found to be related to a certain cellular function. Damages of various, individually functioning membrane systems upon the action of ionizing radiation on the cell are described. Mesosomes are absent from the cells if the synthesis of peptidoglycan is inhibited, whereas hypersynthesis of the cell wall material is observed upon their hypertrophy. The content of mitochondrial analogs in the cell increases with the rate of metabolic processes. The function of cellular division is disturbed as a result of damages in the nucleoid DNA structure and degradation of nucleoidosomes. Changes in carbohydrate and lipid metabolism are also observed."}
{"id": "PMID:470634", "title": "[Microbiologic studies of a spodumene deposit].", "content": "A wide spectrum of heterotrophic and autotrophic microorganisms was detected in the zones of decomposition of spodumene and bed rocks, pegmatites and shales, in the spodumene deposit. The following aerobic microorganisms which did not from spores predominated in the deposit: Arthrobacter globiformis, A. pascens, A. simplex, Nocardia globerula, Pseudomonas fluorescens, Ps. putida, Ps. testosteronii. The following specific bacterial groups were found: thionic, sulfate reducing, and nitrifying bacteria. Degradation of spodumene and bed rocks was found to occur in moist regions containing cracks; it was accompanied with a decrease in pH. A possible role of microorganisms in decomposition of spodumene as well as removal of elements is discussed.", "contents": "[Microbiologic studies of a spodumene deposit]. A wide spectrum of heterotrophic and autotrophic microorganisms was detected in the zones of decomposition of spodumene and bed rocks, pegmatites and shales, in the spodumene deposit. The following aerobic microorganisms which did not from spores predominated in the deposit: Arthrobacter globiformis, A. pascens, A. simplex, Nocardia globerula, Pseudomonas fluorescens, Ps. putida, Ps. testosteronii. The following specific bacterial groups were found: thionic, sulfate reducing, and nitrifying bacteria. Degradation of spodumene and bed rocks was found to occur in moist regions containing cracks; it was accompanied with a decrease in pH. A possible role of microorganisms in decomposition of spodumene as well as removal of elements is discussed."}
{"id": "PMID:470635", "title": "[Amination and biosynthesis of glutamate by R- and S-forms of Bacillus thuringiensis].", "content": "These studies were designed to determine the biochemical nature of the Bacillus thuringiensis growth being dependent on glutamate during cultivation in a minimal medium. This is possible to be due to the absence of enzymes which catalyze glutamic acid synthesis by direct amination of alpha-ketoglutaric acid, glutamate dehydrogenase and glutamate synthase, and a decrease in the activity of the enzyme catalyzing amination of pyruvic acid, alanine dehydrogenase. It has been shown that the lack of glutamate can be compensated by histidine and proline; in this case, the growth efficiency of R form is greater than that of S form which is consistent with an increased rate of protein synthesis of R form.", "contents": "[Amination and biosynthesis of glutamate by R- and S-forms of Bacillus thuringiensis]. These studies were designed to determine the biochemical nature of the Bacillus thuringiensis growth being dependent on glutamate during cultivation in a minimal medium. This is possible to be due to the absence of enzymes which catalyze glutamic acid synthesis by direct amination of alpha-ketoglutaric acid, glutamate dehydrogenase and glutamate synthase, and a decrease in the activity of the enzyme catalyzing amination of pyruvic acid, alanine dehydrogenase. It has been shown that the lack of glutamate can be compensated by histidine and proline; in this case, the growth efficiency of R form is greater than that of S form which is consistent with an increased rate of protein synthesis of R form."}
{"id": "PMID:470636", "title": "[Crocoite reduction by a culture of Pseudomonas chromatophila sp. nov].", "content": "Two cultures of chromium reducing bacteria have been isolated from samples of water accumulated at the bottom of an open-cut chromium deposit and from industrial sewage. The cultures were found to be identical. The cultures are described and their taxonomical position is discussed. They have been classed as Psuedomonas chromatophila sp. nov. the cultures reduce Cr(VI) from crocoite PbCrO4 to Cr(III). A possibility of chromium reducing bacteria being involved in the conversion of crocoite to chromite in the oxidation zone of chromite deposits is discussed.", "contents": "[Crocoite reduction by a culture of Pseudomonas chromatophila sp. nov]. Two cultures of chromium reducing bacteria have been isolated from samples of water accumulated at the bottom of an open-cut chromium deposit and from industrial sewage. The cultures were found to be identical. The cultures are described and their taxonomical position is discussed. They have been classed as Psuedomonas chromatophila sp. nov. the cultures reduce Cr(VI) from crocoite PbCrO4 to Cr(III). A possibility of chromium reducing bacteria being involved in the conversion of crocoite to chromite in the oxidation zone of chromite deposits is discussed."}
{"id": "PMID:470638", "title": "[Bacterial destruction of synthetic organic flotation agents].", "content": "Microorganisms that use the flotation agent T-66 as the sole source of carbon have been isolated from soil enriched with this agent. A mixture of bacterial cultures belonging to the genus Pseudomonas (Ps. fluorescens, Ps. desmolyticum, Ps. rathonis, Ps. cyanoides viscosa, and Ps. aeruginosa) oxidized, after adaptation, at a high rate, components of the flotation agent T-66. Aeration of the medium accelerated the destruction. About 90% of the foltation agent components were oxidized within four months. The highest activity was observed during the first two months. The bacteria also decomposed, actively, oxidized kerosene and oxidized recycle stock added as the sole source of carbon to a mineral medium. Introduction of glucose to the medium accelerated destruction of oxidized recycle stock by the microorganisms, but inhibited destruction of oxidized kerosene.", "contents": "[Bacterial destruction of synthetic organic flotation agents]. Microorganisms that use the flotation agent T-66 as the sole source of carbon have been isolated from soil enriched with this agent. A mixture of bacterial cultures belonging to the genus Pseudomonas (Ps. fluorescens, Ps. desmolyticum, Ps. rathonis, Ps. cyanoides viscosa, and Ps. aeruginosa) oxidized, after adaptation, at a high rate, components of the flotation agent T-66. Aeration of the medium accelerated the destruction. About 90% of the foltation agent components were oxidized within four months. The highest activity was observed during the first two months. The bacteria also decomposed, actively, oxidized kerosene and oxidized recycle stock added as the sole source of carbon to a mineral medium. Introduction of glucose to the medium accelerated destruction of oxidized recycle stock by the microorganisms, but inhibited destruction of oxidized kerosene."}
{"id": "PMID:470639", "title": "[Systematics of acetic acid bacteria].", "content": "The method of numerical taxonomy has been used in these studies. Fifty-six strains of acetic acid bacteria are characterized in 136 phenotypical features. The coefficients of similarity of the strains were calculated using computer. The nucleotide composition of DNA from 21 strains of acetic acid bacteria was determined. The results obtained by the method of numerical taxonomy were discussed basing on this genotypical criterion. The genera Acetobacter Beijerinck and Gluconobacter Asai were found to be related. Only one bacterial species, i. e. G. oxydans, was confirmed to exist within the genus Gluconobacter. The studied strains belonging to the genus Acetobacter were subdivided into three groups corresponding to the species A. aceti, A. rancens and A. xylinum.", "contents": "[Systematics of acetic acid bacteria]. The method of numerical taxonomy has been used in these studies. Fifty-six strains of acetic acid bacteria are characterized in 136 phenotypical features. The coefficients of similarity of the strains were calculated using computer. The nucleotide composition of DNA from 21 strains of acetic acid bacteria was determined. The results obtained by the method of numerical taxonomy were discussed basing on this genotypical criterion. The genera Acetobacter Beijerinck and Gluconobacter Asai were found to be related. Only one bacterial species, i. e. G. oxydans, was confirmed to exist within the genus Gluconobacter. The studied strains belonging to the genus Acetobacter were subdivided into three groups corresponding to the species A. aceti, A. rancens and A. xylinum."}
{"id": "PMID:470644", "title": "Large 'small' groups: their place in higher education.", "content": "Small group teaching in higher education appears to be an ideal worth aiming for. The review of the literature in this paper shows that factural information about the effectiveness of such teaching is far from prolific. In addition, what may be the most common form of small group, viz. the 'seminar', has received far less attention than the 'lecture' or 'tutorial'. Special attention is drawn to the relevance of the studies for medical education but the overall finding is that need for well designed evaluative research is urgent, particularly with regard to 'seminar' learning groups.", "contents": "Large 'small' groups: their place in higher education. Small group teaching in higher education appears to be an ideal worth aiming for. The review of the literature in this paper shows that factural information about the effectiveness of such teaching is far from prolific. In addition, what may be the most common form of small group, viz. the 'seminar', has received far less attention than the 'lecture' or 'tutorial'. Special attention is drawn to the relevance of the studies for medical education but the overall finding is that need for well designed evaluative research is urgent, particularly with regard to 'seminar' learning groups."}
{"id": "PMID:470645", "title": "The value of a perception and control test in predicting performance in dental students and ancillary personnel.", "content": "A test designed to measure basic manual perception and control skills, assumed to be less susceptible to the effects of haphazard and unplanned training, was developed and used with groups of dental students and ancillary personnel. The test was similar to one reported to be successful in predicting practical ability in American dental students. Statistical techniques were used to compare the results of the perception and control test with practical and theory examination results. The results show that the perception and control test is not a reliable predictor of performance with British dental students but was more successful with the ancillary groups and particularly with dental auxiliaries.", "contents": "The value of a perception and control test in predicting performance in dental students and ancillary personnel. A test designed to measure basic manual perception and control skills, assumed to be less susceptible to the effects of haphazard and unplanned training, was developed and used with groups of dental students and ancillary personnel. The test was similar to one reported to be successful in predicting practical ability in American dental students. Statistical techniques were used to compare the results of the perception and control test with practical and theory examination results. The results show that the perception and control test is not a reliable predictor of performance with British dental students but was more successful with the ancillary groups and particularly with dental auxiliaries."}
{"id": "PMID:470640", "title": "[Total number and biomass of microorganisms in the depths of the Black Sea].", "content": "The total number and biomass of microorganisms, as well as the ratio between their main morphological forms, were determined by capillary microscopy in 22 water samples taken at various depths from the surface to 1500 m in the eastern part of the Black Sea. The total number of microorganisms was several times higher at depths over 150 m in the hydrogen sulfide zone than in the oxygen zone (0--150 m), whereas the biomass was greater by an order of magnitude. The number of microorganisms varied within  a range of 1.4--12.4X10(3) cells/ml in the oxygen zone and 3.8--34.9X10(3) cells/ml in the hydrogen sulfide zone, whereas the biomass was 0.9--6.9 mg/m3 and 10.8--55.6 mg/m3, respectively. The bulk of the microbial biomass in the hydrogen sulfide zone was constituted by peculiar filamentous forms described for the first time by Lebedeva (1953, 1963) and, apparently, participating in sulfur turnover.", "contents": "[Total number and biomass of microorganisms in the depths of the Black Sea]. The total number and biomass of microorganisms, as well as the ratio between their main morphological forms, were determined by capillary microscopy in 22 water samples taken at various depths from the surface to 1500 m in the eastern part of the Black Sea. The total number of microorganisms was several times higher at depths over 150 m in the hydrogen sulfide zone than in the oxygen zone (0--150 m), whereas the biomass was greater by an order of magnitude. The number of microorganisms varied within  a range of 1.4--12.4X10(3) cells/ml in the oxygen zone and 3.8--34.9X10(3) cells/ml in the hydrogen sulfide zone, whereas the biomass was 0.9--6.9 mg/m3 and 10.8--55.6 mg/m3, respectively. The bulk of the microbial biomass in the hydrogen sulfide zone was constituted by peculiar filamentous forms described for the first time by Lebedeva (1953, 1963) and, apparently, participating in sulfur turnover."}
{"id": "PMID:470646", "title": "Clinical problem solving and clinical knowledge.", "content": "Videotape and simulated patients were used for evaluating third year medical students' performances in solving familiar and unfamiliar clinical problems. To assist the doctor to rate the students' performances by the review of videotapes, criteria were set up for students' behaviours that could be construed as evidence that the students recognized cues obtained by interviewing the patient and the information content of such cues. Criteria for data use by the students were also set. A mean of 90% of the pertinent medical history data was collected by the students. The amount of pertinent historical data collected was found to correlate very closely with student-patient interaction analysis score for the familiar and unfamiliar problems (r = 0.89 and r = 0.92, respectively). Significant differences in mean scores for data generation (P less than 0.001) and for data use (P less than 0.01) were found between those students who solved and those who failed to solve the familiar problem. With the unfamiliar problem a significant difference was found between the mean scores for data generation (P less than 0.01), but not for data use. The implications of these findings in medical education are discussed.", "contents": "Clinical problem solving and clinical knowledge. Videotape and simulated patients were used for evaluating third year medical students' performances in solving familiar and unfamiliar clinical problems. To assist the doctor to rate the students' performances by the review of videotapes, criteria were set up for students' behaviours that could be construed as evidence that the students recognized cues obtained by interviewing the patient and the information content of such cues. Criteria for data use by the students were also set. A mean of 90% of the pertinent medical history data was collected by the students. The amount of pertinent historical data collected was found to correlate very closely with student-patient interaction analysis score for the familiar and unfamiliar problems (r = 0.89 and r = 0.92, respectively). Significant differences in mean scores for data generation (P less than 0.001) and for data use (P less than 0.01) were found between those students who solved and those who failed to solve the familiar problem. With the unfamiliar problem a significant difference was found between the mean scores for data generation (P less than 0.01), but not for data use. The implications of these findings in medical education are discussed."}
{"id": "PMID:470647", "title": "A comparison of multiple-choice tests and free-response tests in examinations of clinical competence.", "content": "This paper reports a study which compared the performance of different groups of students and doctors on identical and equivalent tests set in an objective-type format and in a free-response format. The tests were designed to ensure that the content was relevant to clinical practice at the hospital intern level. In all test situations candidates' scores were significantly higher in the objective tests than in the free-response tests. This difference was greater for the more junior and less competent students than for the more competent doctors. The cueing effect of the options was thought to be the main factor responsible for the difference in performance. The results of a questionnaire survey demonstrated that students were aware of the deficiencies in multiple-choice tests. A large majority of the students believed that the free-response tests gave a more accurate assessment of their clinical ability. It was found that in these tests, aimed at measuring aspects of clinical competence, multiple-choice questions appeared to overestimate the candidate's ability to an extent that made them less suitable than free-response questions for this purpose. It was also found that free-response tests, of the type used in this study, provide a suitable alternative to multiple-choice tests for use in the written section of clinical examinations. It was concluded that the written component of the final examination in the medical course should have a preponderance of free-response items over multiple-choice items.", "contents": "A comparison of multiple-choice tests and free-response tests in examinations of clinical competence. This paper reports a study which compared the performance of different groups of students and doctors on identical and equivalent tests set in an objective-type format and in a free-response format. The tests were designed to ensure that the content was relevant to clinical practice at the hospital intern level. In all test situations candidates' scores were significantly higher in the objective tests than in the free-response tests. This difference was greater for the more junior and less competent students than for the more competent doctors. The cueing effect of the options was thought to be the main factor responsible for the difference in performance. The results of a questionnaire survey demonstrated that students were aware of the deficiencies in multiple-choice tests. A large majority of the students believed that the free-response tests gave a more accurate assessment of their clinical ability. It was found that in these tests, aimed at measuring aspects of clinical competence, multiple-choice questions appeared to overestimate the candidate's ability to an extent that made them less suitable than free-response questions for this purpose. It was also found that free-response tests, of the type used in this study, provide a suitable alternative to multiple-choice tests for use in the written section of clinical examinations. It was concluded that the written component of the final examination in the medical course should have a preponderance of free-response items over multiple-choice items."}
{"id": "PMID:470648", "title": "Occupational medicine: student evaluation of a postgraduate course.", "content": "Postgraduate students attending a ten-week course in occupational medicine have evaluated all contributions to this course on the basis of value, interest and presentation. Assessments given for the standards of presentation of items of the teaching vary more than the assessments given for interest. Interest in those parts of the course with a direct clinical content is greater than in other sections of the course. Methodological problems necessitate certain assumptions in this form of audit. The results nevertheless provide worthwhile feedback to contributors and co-ordinators that can be used to maintain and improve the quality of courses.", "contents": "Occupational medicine: student evaluation of a postgraduate course. Postgraduate students attending a ten-week course in occupational medicine have evaluated all contributions to this course on the basis of value, interest and presentation. Assessments given for the standards of presentation of items of the teaching vary more than the assessments given for interest. Interest in those parts of the course with a direct clinical content is greater than in other sections of the course. Methodological problems necessitate certain assumptions in this form of audit. The results nevertheless provide worthwhile feedback to contributors and co-ordinators that can be used to maintain and improve the quality of courses."}
{"id": "PMID:470649", "title": "The careers of women graduates from St Mary's Hospital Medical School, London, 1961--72.", "content": "The careers of women doctors who qualified from St Mary's Hospital Medical School between 1961 and 1972 inclusive have been studied. Thirty-eight per cent were in full-time work, 47% were working part-time, and 15% were not practising medicine at the time of the survey. Those working full-time were predominantly single women and married women with no children. With the birth of children most women stopped working for a time, and 38% of those whose children were all under school age were not working. However, 90% returned to medicine, usually to part-time jobs that were compatible with family responsibilities. Eighty-six per cent of the respondents held one or more postgraduate qualifications. More of those with higher qualifications were in full-time work than was the case for women with a basic medical degree only, and fewer were not practising medicine. An equal proportion of single and married women intended to make their career in general practice. Fewer married women than single women chose a hospital career, because the possibilities of part-time work in this field were seen as limited.", "contents": "The careers of women graduates from St Mary's Hospital Medical School, London, 1961--72. The careers of women doctors who qualified from St Mary's Hospital Medical School between 1961 and 1972 inclusive have been studied. Thirty-eight per cent were in full-time work, 47% were working part-time, and 15% were not practising medicine at the time of the survey. Those working full-time were predominantly single women and married women with no children. With the birth of children most women stopped working for a time, and 38% of those whose children were all under school age were not working. However, 90% returned to medicine, usually to part-time jobs that were compatible with family responsibilities. Eighty-six per cent of the respondents held one or more postgraduate qualifications. More of those with higher qualifications were in full-time work than was the case for women with a basic medical degree only, and fewer were not practising medicine. An equal proportion of single and married women intended to make their career in general practice. Fewer married women than single women chose a hospital career, because the possibilities of part-time work in this field were seen as limited."}
{"id": "PMID:470650", "title": "Special provisions for women doctors to train and practise in medicine after graduation: a report of a survey.", "content": "The findings of a survey of 3262 women doctors registered between 1945 and 1974 are reported. The results are based on 2433 returned questionnaires (75% response). The survey confirmed that the special forms of assistance provided to enable women doctors to continue training and to work after graduation are inadequate. Although the Women Doctors' Retainer Scheme and part-time training schemes are suited to women's needs, they are insufficiently publicized, inflexibly administered and limited in availability. The need for a counselling service for women doctors is not met. Facilities for child care on NHS premises are very deficient. With more substantial provision of all these forms of assistance to women doctors, the scope and extent of their contribution to medicine could be increased. The Medical Register is an unreliable means of ascertaining the numbers and whereabouts of women who may require this special assistance.  The main problem now is not a lack of ideas about suitable provision for women doctors, but the implementation by the relevant authorities of the proposals already made, to enable both women doctors themselves and the NHS to benefit.", "contents": "Special provisions for women doctors to train and practise in medicine after graduation: a report of a survey. The findings of a survey of 3262 women doctors registered between 1945 and 1974 are reported. The results are based on 2433 returned questionnaires (75% response). The survey confirmed that the special forms of assistance provided to enable women doctors to continue training and to work after graduation are inadequate. Although the Women Doctors' Retainer Scheme and part-time training schemes are suited to women's needs, they are insufficiently publicized, inflexibly administered and limited in availability. The need for a counselling service for women doctors is not met. Facilities for child care on NHS premises are very deficient. With more substantial provision of all these forms of assistance to women doctors, the scope and extent of their contribution to medicine could be increased. The Medical Register is an unreliable means of ascertaining the numbers and whereabouts of women who may require this special assistance.  The main problem now is not a lack of ideas about suitable provision for women doctors, but the implementation by the relevant authorities of the proposals already made, to enable both women doctors themselves and the NHS to benefit."}
{"id": "PMID:470652", "title": "Perception of teaching competencies by medical educators.", "content": "The purpose of this study was to determine the effect of an introductory educational planning workshop on perception of teaching competencies by medical educators. A questionnaire containing twenty-seven teaching competencies (TC) was administered before and after the workshop. The twenty-five participants, from four different countries, were asked to rate each TC regarding its importance for teachers and whether they possessed them or needed further training. The results showed that this workshop created more awareness regarding the importance of these TCs and also created a need for further training for specific TCs in order to put educational principles into practice.", "contents": "Perception of teaching competencies by medical educators. The purpose of this study was to determine the effect of an introductory educational planning workshop on perception of teaching competencies by medical educators. A questionnaire containing twenty-seven teaching competencies (TC) was administered before and after the workshop. The twenty-five participants, from four different countries, were asked to rate each TC regarding its importance for teachers and whether they possessed them or needed further training. The results showed that this workshop created more awareness regarding the importance of these TCs and also created a need for further training for specific TCs in order to put educational principles into practice."}
{"id": "PMID:470666", "title": "Health status of Seventh-Day Adventists.", "content": "A comparison of health status between 779 Seventh-day Adventists, who have a strong commitment to heal-related life styles, and two other groups of people--8363 persons referred by general practitioners and 9825 volunteers--was made. The Seventh-day Adventists showed less impairment of systolic and diastolic blood pressures, of plasma cholesterol and plasma urate concentrations, and of lung ventilatory capacity; and less obesity at most specific ages. With increasing age, the level of breathlessness, reported heart disease, hypertension, and hypertensive and diuretic therapy in this sample approached that of the comparative groups, possibly because of natural attrition of high-risk persons in the latter. Depression, sleeplessness, use of sedatives and tranquillizers were lower in the Seventh-day Adventists; although, once again, a drawing together of the three groups in older age categories was evident. It is concluded that the life style of Seventh-day Adventists is conducive to lessened morbidity, delayed mortality, and decreased call on health services in comparison with the general population.", "contents": "Health status of Seventh-Day Adventists. A comparison of health status between 779 Seventh-day Adventists, who have a strong commitment to heal-related life styles, and two other groups of people--8363 persons referred by general practitioners and 9825 volunteers--was made. The Seventh-day Adventists showed less impairment of systolic and diastolic blood pressures, of plasma cholesterol and plasma urate concentrations, and of lung ventilatory capacity; and less obesity at most specific ages. With increasing age, the level of breathlessness, reported heart disease, hypertension, and hypertensive and diuretic therapy in this sample approached that of the comparative groups, possibly because of natural attrition of high-risk persons in the latter. Depression, sleeplessness, use of sedatives and tranquillizers were lower in the Seventh-day Adventists; although, once again, a drawing together of the three groups in older age categories was evident. It is concluded that the life style of Seventh-day Adventists is conducive to lessened morbidity, delayed mortality, and decreased call on health services in comparison with the general population."}
{"id": "PMID:470667", "title": "Prescription drug advertising: medical journal practices under different types of control.", "content": "Medication is one important aspect of health care delivery. The sources of information used to select appropriate medication include drug advertisements which appear in professional journals. There is a need to monitor the quality of these advertisements. The study reported in this paper includes a longitudinal (1961 to 1977) and cross-societal (Britain, United States and Australia) content analysis of drug advertisements. Such a comparison contributes to the debate concerning the type of regulation likely to be effective for this form of advertising.", "contents": "Prescription drug advertising: medical journal practices under different types of control. Medication is one important aspect of health care delivery. The sources of information used to select appropriate medication include drug advertisements which appear in professional journals. There is a need to monitor the quality of these advertisements. The study reported in this paper includes a longitudinal (1961 to 1977) and cross-societal (Britain, United States and Australia) content analysis of drug advertisements. Such a comparison contributes to the debate concerning the type of regulation likely to be effective for this form of advertising."}
{"id": "PMID:470662", "title": "Knee impedance testing machine.", "content": "A device is described that measures the mechanical impedance of the human knee in vivo. The device and method were tested in a reliability study, on a normal control population, and in a follow-up test of previously injured subjects. Finally, impedance measurement was compared with the clinical examination, the currently employed test for ligament injuries. This impedance method to measure ligament stiffness was found useful in diagnosing acute injuries.", "contents": "Knee impedance testing machine. A device is described that measures the mechanical impedance of the human knee in vivo. The device and method were tested in a reliability study, on a normal control population, and in a follow-up test of previously injured subjects. Finally, impedance measurement was compared with the clinical examination, the currently employed test for ligament injuries. This impedance method to measure ligament stiffness was found useful in diagnosing acute injuries."}
{"id": "PMID:470668", "title": "Hodgkin's disease: evaluation of staging procedures for abdominal involvement.", "content": "Results of preoperative assessment procedures and subsequent staging laparotomy are evaluated in 32 consecutive patients who presented with extra-abdominal Hodgkin's disease which was not already known to be in an advanced stage. Disease was found in the abdominal cavity of 10 of the 27 patients who presented with disease above the diaphragm, and of two of the five patients in whom disease presented in the inguinal nodes. In patients who initially presented with disease above the diaphragm, the spleen was involved in all 10 cases where abdominal disease was detected. Lymphography was not found to be reliable as a diagnostic aid in doubtful cases. Neither liver nor spleen scans were found to be helpful in assessment of these patients.", "contents": "Hodgkin's disease: evaluation of staging procedures for abdominal involvement. Results of preoperative assessment procedures and subsequent staging laparotomy are evaluated in 32 consecutive patients who presented with extra-abdominal Hodgkin's disease which was not already known to be in an advanced stage. Disease was found in the abdominal cavity of 10 of the 27 patients who presented with disease above the diaphragm, and of two of the five patients in whom disease presented in the inguinal nodes. In patients who initially presented with disease above the diaphragm, the spleen was involved in all 10 cases where abdominal disease was detected. Lymphography was not found to be reliable as a diagnostic aid in doubtful cases. Neither liver nor spleen scans were found to be helpful in assessment of these patients."}
{"id": "PMID:470669", "title": "Childhood asthma and grass pollen aerobiology in Melbourne.", "content": "Children from the Melbourne metropolitan area with laboratory proven bronchial sensitivity to ryegrass pollen were selected for a clinical trial which was run concurrently with an aerobiological survey during the grass pollen season of 1975 to 1976. During periods of high pollen counts, significant deterioration in the children's asthma occurred. The grass pollen season was of 10 weeks' duration with the highest daily counts during November and December. Highest diurnal pollen concentrations occurred at night and were associated with winds from the north, north-west, and south-east and with other meteorological factors.", "contents": "Childhood asthma and grass pollen aerobiology in Melbourne. Children from the Melbourne metropolitan area with laboratory proven bronchial sensitivity to ryegrass pollen were selected for a clinical trial which was run concurrently with an aerobiological survey during the grass pollen season of 1975 to 1976. During periods of high pollen counts, significant deterioration in the children's asthma occurred. The grass pollen season was of 10 weeks' duration with the highest daily counts during November and December. Highest diurnal pollen concentrations occurred at night and were associated with winds from the north, north-west, and south-east and with other meteorological factors."}
{"id": "PMID:470670", "title": "A comparison of various parameters of social grade: a study of a Sydney population.", "content": "A comparison has been made between different methods of classifying a Sydney population of 118 persons into social grades. A positive correlation was found between address and occupation, education and occupation, occupation and father's occupation, address and education, address and father's occupation, and education and father's occupation, all correlations being significant at or below the P=0.004 level of statistical significance.", "contents": "A comparison of various parameters of social grade: a study of a Sydney population. A comparison has been made between different methods of classifying a Sydney population of 118 persons into social grades. A positive correlation was found between address and occupation, education and occupation, occupation and father's occupation, address and education, address and father's occupation, and education and father's occupation, all correlations being significant at or below the P=0.004 level of statistical significance."}
{"id": "PMID:470687", "title": "Elastic stockings in the control of hand oedema.", "content": "Elastic support and compression in the form of an elastic finger stall or glove seems to be a simple and comfortable way to obviate and reduce oedema of the hand and fingers after surgery or trauma. It may also prove useful in inflammatory conditions such as rheumatoid arthritis, though the authors have not used it as yet for that condition.", "contents": "Elastic stockings in the control of hand oedema. Elastic support and compression in the form of an elastic finger stall or glove seems to be a simple and comfortable way to obviate and reduce oedema of the hand and fingers after surgery or trauma. It may also prove useful in inflammatory conditions such as rheumatoid arthritis, though the authors have not used it as yet for that condition."}
{"id": "PMID:470688", "title": "Sequential intellectual recovery after near-drowning.", "content": "Sequential neurological and intellectual recovery after childhood near-drowning is discussed. Decisions concerning the persistence and intensity of resuscitation require a knowledge of the natural history of intellectual improvement after rescue from near-drowning. A severe case of fresh-water immersion, leading to recovery, is described. Evidence is presented to suggest that the time interval of one hour before the first spontaneous respiratory gasp forms the upper limit of the apnoeic time bracket after which survival can still be expected, and to indicate that intellectual improvement (to a measured IQ of 97) can occur even after initial decerebrate signs if vigorous therapy is prosecuted. The proportion of cases capable of sequential neurological improvement is unknown. A time base for sequential clinical and intellectual improvement after near-drowning is presented to form a yardstick with which future cases may be compared.", "contents": "Sequential intellectual recovery after near-drowning. Sequential neurological and intellectual recovery after childhood near-drowning is discussed. Decisions concerning the persistence and intensity of resuscitation require a knowledge of the natural history of intellectual improvement after rescue from near-drowning. A severe case of fresh-water immersion, leading to recovery, is described. Evidence is presented to suggest that the time interval of one hour before the first spontaneous respiratory gasp forms the upper limit of the apnoeic time bracket after which survival can still be expected, and to indicate that intellectual improvement (to a measured IQ of 97) can occur even after initial decerebrate signs if vigorous therapy is prosecuted. The proportion of cases capable of sequential neurological improvement is unknown. A time base for sequential clinical and intellectual improvement after near-drowning is presented to form a yardstick with which future cases may be compared."}
{"id": "PMID:470689", "title": "Choreiform movements induced by anticholinergic therapy.", "content": "A case is reported where long term therapy with anticholinergic drugs caused choreiform movements in a 73-year-old man. After the therapy was ceased the movements disappeared. The elderly are particularly at risk from developing side effects from drug therapy. We believe this to be the first report of chorea produced by long-term therapy with benzhexol.", "contents": "Choreiform movements induced by anticholinergic therapy. A case is reported where long term therapy with anticholinergic drugs caused choreiform movements in a 73-year-old man. After the therapy was ceased the movements disappeared. The elderly are particularly at risk from developing side effects from drug therapy. We believe this to be the first report of chorea produced by long-term therapy with benzhexol."}
{"id": "PMID:470690", "title": "Rapid cholesterol measurement: patient classification in heart risk evaluation clinics.", "content": "Plasma cholesterol levels of patients attending a heart risk evaluation clinic were measured both in the clinic at the time of attendance, and later in a reference laboratory. The workflow of evaluation clinics may be improved, and patient satisfaction enhanced, by in situ measurement, since more than 60% of patients can be given a complete risk score at the time of attendance.", "contents": "Rapid cholesterol measurement: patient classification in heart risk evaluation clinics. Plasma cholesterol levels of patients attending a heart risk evaluation clinic were measured both in the clinic at the time of attendance, and later in a reference laboratory. The workflow of evaluation clinics may be improved, and patient satisfaction enhanced, by in situ measurement, since more than 60% of patients can be given a complete risk score at the time of attendance."}
{"id": "PMID:470692", "title": "Effect of high-energy feedings on low birthweight infants.", "content": "A comparison of the effect of 100.5 kJ/30 mL (24 kcal/30 mL) feedings with that of 84 kJ/30 mL (20 kcal/30 mL) feedings on infants with a birthweight of 1500 g or less was made. No consistent benefits in growth occurred and there was a tendency for an increase in complications in infants fed the 100.5 kJ/30 mL milk. Hence the higher-energy feeding cannot be recommended as the routine feeding for low birthweight infants.", "contents": "Effect of high-energy feedings on low birthweight infants. A comparison of the effect of 100.5 kJ/30 mL (24 kcal/30 mL) feedings with that of 84 kJ/30 mL (20 kcal/30 mL) feedings on infants with a birthweight of 1500 g or less was made. No consistent benefits in growth occurred and there was a tendency for an increase in complications in infants fed the 100.5 kJ/30 mL milk. Hence the higher-energy feeding cannot be recommended as the routine feeding for low birthweight infants."}
{"id": "PMID:470693", "title": "Copper 7 devices devoid of copper: implications for duration of useful life.", "content": "Two copper 7 intrauterine devices were found to be devoid of copper on removal after 55 and 63 months of use respectively. The implications of this observation are discussed in relation to data regarding the useful lifespan of such contraceptive devices.", "contents": "Copper 7 devices devoid of copper: implications for duration of useful life. Two copper 7 intrauterine devices were found to be devoid of copper on removal after 55 and 63 months of use respectively. The implications of this observation are discussed in relation to data regarding the useful lifespan of such contraceptive devices."}
{"id": "PMID:470694", "title": "The effect of termination of pregnancy on maturity of subsequent pregnancy.", "content": "Therapeutic termination of pregnancy performed during the first trimester was associated with a statistically significant increase in the incidence of second trimester abortion and of premature labour in the next pregnancy. In a series of 520 patients who had previously been aborted 8.1% suffered a mid-trimester abortion in their next pregnancy and 8.6% had premature onset of labour (compared to 2.4% (P less than 0.001) and 4.4% (P less than 0.01) respectively in a control series). The incidence of incompetence of the cervix after termination of pregnancy was 4.4%.", "contents": "The effect of termination of pregnancy on maturity of subsequent pregnancy. Therapeutic termination of pregnancy performed during the first trimester was associated with a statistically significant increase in the incidence of second trimester abortion and of premature labour in the next pregnancy. In a series of 520 patients who had previously been aborted 8.1% suffered a mid-trimester abortion in their next pregnancy and 8.6% had premature onset of labour (compared to 2.4% (P less than 0.001) and 4.4% (P less than 0.01) respectively in a control series). The incidence of incompetence of the cervix after termination of pregnancy was 4.4%."}
{"id": "PMID:470695", "title": "Tetanus and psychiatry: unexpected bedfellows.", "content": "Six patients who were admitted with the initial diagnosis of tetanus to a general hospital serving 130 000 people over a 10-year period are reviewed. In three of these patients psychogenic factors played a major part in the presenting pictures. These cases followed a markedly different course from the others. Guidelines to the early differentiation of such atypical cases, and to the way psychogenic factors can be identified and their importance evaluated, are discussed.", "contents": "Tetanus and psychiatry: unexpected bedfellows. Six patients who were admitted with the initial diagnosis of tetanus to a general hospital serving 130 000 people over a 10-year period are reviewed. In three of these patients psychogenic factors played a major part in the presenting pictures. These cases followed a markedly different course from the others. Guidelines to the early differentiation of such atypical cases, and to the way psychogenic factors can be identified and their importance evaluated, are discussed."}
{"id": "PMID:470696", "title": "Survival in subzero temperatures: two field experiments on temperature estimation and \"snowhole\" (survival hole) temperatures.", "content": "Two field experiments in a subarctic environment are described. Individuals consistently underestimated the degree of cold, because visual cues dominated sensory skin receptors in the subjective estimation of temperature. In snowholes (survival holes) temperatures rose 20 degrees C within 30 minutes of occupancy, but stabilized at freezing point. Implications for travel and survival in a subzero environment are described.", "contents": "Survival in subzero temperatures: two field experiments on temperature estimation and \"snowhole\" (survival hole) temperatures. Two field experiments in a subarctic environment are described. Individuals consistently underestimated the degree of cold, because visual cues dominated sensory skin receptors in the subjective estimation of temperature. In snowholes (survival holes) temperatures rose 20 degrees C within 30 minutes of occupancy, but stabilized at freezing point. Implications for travel and survival in a subzero environment are described."}
{"id": "PMID:470697", "title": "Perhexiline maleate in the treatment of severe angina pectoris.", "content": "Perhexiline maleate was used as a prophylactic agent in 26 patients suffering from severe angina pectoris. The mean duration of treatment was 8.9 months, with a maximum of 28 months. Fifteen patients experienced a reduction in frequency of attacks to less than one-third of their previous level; six experienced a reduction to two-thirds of their previous level; no patient showed an increase in attack rates. During the period of study, there was one death. Frequently observed side effects included dizziness, gastrointestinal irritation and malaise. One patient developed clinically apparent hepatic dysfunction which resolved on withdrawal of perhexiline maleate, but recurred after rechallenge with a lower dose of the drug; the results of liver function tests in five others showed mild abnormalities. One patient developed peripheral neuropathy after taking perhexiline maleate for 18 months, but this resolved in two months after cessation of therapy. Good responses to perhexiline maleate were observed in patients who were concurrently treated with beta-adrenoreceptor blocking drugs.", "contents": "Perhexiline maleate in the treatment of severe angina pectoris. Perhexiline maleate was used as a prophylactic agent in 26 patients suffering from severe angina pectoris. The mean duration of treatment was 8.9 months, with a maximum of 28 months. Fifteen patients experienced a reduction in frequency of attacks to less than one-third of their previous level; six experienced a reduction to two-thirds of their previous level; no patient showed an increase in attack rates. During the period of study, there was one death. Frequently observed side effects included dizziness, gastrointestinal irritation and malaise. One patient developed clinically apparent hepatic dysfunction which resolved on withdrawal of perhexiline maleate, but recurred after rechallenge with a lower dose of the drug; the results of liver function tests in five others showed mild abnormalities. One patient developed peripheral neuropathy after taking perhexiline maleate for 18 months, but this resolved in two months after cessation of therapy. Good responses to perhexiline maleate were observed in patients who were concurrently treated with beta-adrenoreceptor blocking drugs."}
{"id": "PMID:470721", "title": "Lymphoid hyperplasia of small bowel in an adult female.", "content": "A case of terminal ileum lymphoid hyperplasia in an adult female is presented. Symptoms have been alleviated by limited resection of affected ileum and a segment of ascending colon. It appears that this process has been previously reported only in children and young people, most of the patients being male.", "contents": "Lymphoid hyperplasia of small bowel in an adult female. A case of terminal ileum lymphoid hyperplasia in an adult female is presented. Symptoms have been alleviated by limited resection of affected ileum and a segment of ascending colon. It appears that this process has been previously reported only in children and young people, most of the patients being male."}
{"id": "PMID:470722", "title": "A new cricothyrotomy cannula.", "content": "A simple, easily constructed cricothyrotomy cannula and its method of use are described. Experiments have demonstrated that the instrument admits adequate air to sustain life without causing trauma to the posterior laryngeal wall. The dimensions allow the concurrent use of the FG10 suction catheter. An accessory device which enables single instrument cricothyrotomy is also described.", "contents": "A new cricothyrotomy cannula. A simple, easily constructed cricothyrotomy cannula and its method of use are described. Experiments have demonstrated that the instrument admits adequate air to sustain life without causing trauma to the posterior laryngeal wall. The dimensions allow the concurrent use of the FG10 suction catheter. An accessory device which enables single instrument cricothyrotomy is also described."}
{"id": "PMID:470723", "title": "Mammography. An aid or distractor in diagnosis?", "content": "A retrospective analysis of 155 mammograms has indicated the value of this procedure even with simple and inexpensive equipment. In women with a discrete mass requiring biopsy, mammography combined with clinical findings predicted with 100% confidence over half the cancers diagnosed. When both X-ray and clinical findings suggested a benign lump, only one in nine was a carcinoma. In the one-third of patients in whom signs and X-ray findings were in conflict or equivocal, the malignancy rate was about one in three. In women with breast signs or symptoms, but without discrete mass and with a \"benign\" mammography report, no cancer has been found in follow up for from 12 months to seven years.", "contents": "Mammography. An aid or distractor in diagnosis? A retrospective analysis of 155 mammograms has indicated the value of this procedure even with simple and inexpensive equipment. In women with a discrete mass requiring biopsy, mammography combined with clinical findings predicted with 100% confidence over half the cancers diagnosed. When both X-ray and clinical findings suggested a benign lump, only one in nine was a carcinoma. In the one-third of patients in whom signs and X-ray findings were in conflict or equivocal, the malignancy rate was about one in three. In women with breast signs or symptoms, but without discrete mass and with a \"benign\" mammography report, no cancer has been found in follow up for from 12 months to seven years."}
{"id": "PMID:470724", "title": "Carcinoma of the cervix, endometrium, and ovary: an 11-year review.", "content": "Over an 11-year period, 164 patients with genital tract carcinoma were managed at the Austin Hospital, Melbourne. For patients receiving primary treatment at the hospital, the five-year survival rates of patients with carcinoma of the cervix, endometrium, and ovary were 47.8%, 41.6% and 4.3% respectively. Improved results rest upon earlier diagnosis and alternative therapeutic measures, especially for carcinoma of the endometrium and ovary. Routine surveillance after initial therapy may detect asymptomatic metastatic disease when curative treatment is still possible.", "contents": "Carcinoma of the cervix, endometrium, and ovary: an 11-year review. Over an 11-year period, 164 patients with genital tract carcinoma were managed at the Austin Hospital, Melbourne. For patients receiving primary treatment at the hospital, the five-year survival rates of patients with carcinoma of the cervix, endometrium, and ovary were 47.8%, 41.6% and 4.3% respectively. Improved results rest upon earlier diagnosis and alternative therapeutic measures, especially for carcinoma of the endometrium and ovary. Routine surveillance after initial therapy may detect asymptomatic metastatic disease when curative treatment is still possible."}
{"id": "PMID:470725", "title": "Cis-dichlorodiammine platinum (II) in advanced ovarian carcinoma.", "content": "Twenty-seven patients with advanced ovarian cancer were treated with a total of 80 courses of cis-dichlorodiammine platinum (II) (cisplatin). The doses in three courses were less than 50 mg/m2, in 64 courses they were 50 mg/m2, and in eight courses they were 100 mg/m2. In addition, five courses of low-dose daily treatment (20 mg/m2 for five days) were administered. Twelve patients received cisplatin alone, 12 received cisplatin and cyclophosphamide (750 mg/m2), and three patients received cisplatin with other drugs. There were eight responders (33%) in the 24 evaluable patients. Renal toxicity occurred in four patients, ototoxicity in four, and leucopenia (which was life-threatening in one patient) occurred in 16 patients (white cell count less than 3.0 X 10(9)/L). There were two early drug-related deaths. The median survival of patients who responded to cisplatin treatment was longer than 30 weeks compared with nine weeks for non-responders.", "contents": "Cis-dichlorodiammine platinum (II) in advanced ovarian carcinoma. Twenty-seven patients with advanced ovarian cancer were treated with a total of 80 courses of cis-dichlorodiammine platinum (II) (cisplatin). The doses in three courses were less than 50 mg/m2, in 64 courses they were 50 mg/m2, and in eight courses they were 100 mg/m2. In addition, five courses of low-dose daily treatment (20 mg/m2 for five days) were administered. Twelve patients received cisplatin alone, 12 received cisplatin and cyclophosphamide (750 mg/m2), and three patients received cisplatin with other drugs. There were eight responders (33%) in the 24 evaluable patients. Renal toxicity occurred in four patients, ototoxicity in four, and leucopenia (which was life-threatening in one patient) occurred in 16 patients (white cell count less than 3.0 X 10(9)/L). There were two early drug-related deaths. The median survival of patients who responded to cisplatin treatment was longer than 30 weeks compared with nine weeks for non-responders."}
{"id": "PMID:470726", "title": "Indomethacin-induced hyperkalaemia.", "content": "A patient with mild chronic renal failure developed significant hyperkalaemia while receiving indomethacin therapy. The hyperkalaemia, which was reversed by discontinuing indomethacin, is attributed to a drug-induced defect in the cellular uptake of potassium.", "contents": "Indomethacin-induced hyperkalaemia. A patient with mild chronic renal failure developed significant hyperkalaemia while receiving indomethacin therapy. The hyperkalaemia, which was reversed by discontinuing indomethacin, is attributed to a drug-induced defect in the cellular uptake of potassium."}
{"id": "PMID:470734", "title": "Patterns of life after graduation.", "content": "The integration of work and family life is problematic for many doctors. Most of us received little help in resolving the relevant issues during our own formal education. This paper describes a series of informal meetings which were organized for the fifth-year medical students to provide information about possible patterns of work, and to encourage discussion and comment. Areas of particular concern turned out to be postgraduate training, parenting, and the dual-career family. The project was only partly successful.", "contents": "Patterns of life after graduation. The integration of work and family life is problematic for many doctors. Most of us received little help in resolving the relevant issues during our own formal education. This paper describes a series of informal meetings which were organized for the fifth-year medical students to provide information about possible patterns of work, and to encourage discussion and comment. Areas of particular concern turned out to be postgraduate training, parenting, and the dual-career family. The project was only partly successful."}
{"id": "PMID:470735", "title": "Malingering: a dangerous diagnosis.", "content": "Karl Kast, a migrant with a bad back, was examined by four Brisbane orthopaedic surgeons who all independently agreed that there was no physical basis for his complaint. After brooding over this for months, he resolved to kill these doctors and shot three (killing two) before taking his own life. This tragedy and its background are summarized, and a second instance is recorded where a surgeon could have been killed by another accident litigant. There is more to emotional disturbance after injury than a mere quest for compensation. Doctors who hold this simple belief may be at risk if they do not carefully handle the rare cases of severe and dangerous mental illness which masquerade as malingering.", "contents": "Malingering: a dangerous diagnosis. Karl Kast, a migrant with a bad back, was examined by four Brisbane orthopaedic surgeons who all independently agreed that there was no physical basis for his complaint. After brooding over this for months, he resolved to kill these doctors and shot three (killing two) before taking his own life. This tragedy and its background are summarized, and a second instance is recorded where a surgeon could have been killed by another accident litigant. There is more to emotional disturbance after injury than a mere quest for compensation. Doctors who hold this simple belief may be at risk if they do not carefully handle the rare cases of severe and dangerous mental illness which masquerade as malingering."}
{"id": "PMID:470744", "title": "Transanal stapled anastomosis after resection for Hirschsprung's disease.", "content": "A treatment of Hirschsprung's disease in an adult is described. It consisted of rectosigmoidectomy, with continuity being restored by means of a transanal end-to-end stapled anastomosis. The implications of this technique are discussed.", "contents": "Transanal stapled anastomosis after resection for Hirschsprung's disease. A treatment of Hirschsprung's disease in an adult is described. It consisted of rectosigmoidectomy, with continuity being restored by means of a transanal end-to-end stapled anastomosis. The implications of this technique are discussed."}
{"id": "PMID:470745", "title": "Management of urinary incontinence. A new absorbent bed sheet.", "content": "A new, highly absorbent bed sheet (Kylie sheet) was evaluated against cotton drawsheets in the management of incontinence of urine in a crossover study of 19 male and female geriatric patients. When the new Kylie sheets were in use, there was a significant decrease in skin wetness, in disturbance of patients, in nursing workload, and in laundry costs. There were no adverse effects.", "contents": "Management of urinary incontinence. A new absorbent bed sheet. A new, highly absorbent bed sheet (Kylie sheet) was evaluated against cotton drawsheets in the management of incontinence of urine in a crossover study of 19 male and female geriatric patients. When the new Kylie sheets were in use, there was a significant decrease in skin wetness, in disturbance of patients, in nursing workload, and in laundry costs. There were no adverse effects."}
{"id": "PMID:470746", "title": "Relationship between spontaneous abortion and presence of antibody to Toxoplasma gondii.", "content": "Five hundred and twenty-three pregnant women were screened to determine whether a relationship existed between spontaneous abortion, and the presence of antibody to Toxoplasma gondii. The percentage of women with this antibody and a past history of spontaneous miscarriage was not statistically different from the percentage of women without antibody and a past history of spontaneous abortion.", "contents": "Relationship between spontaneous abortion and presence of antibody to Toxoplasma gondii. Five hundred and twenty-three pregnant women were screened to determine whether a relationship existed between spontaneous abortion, and the presence of antibody to Toxoplasma gondii. The percentage of women with this antibody and a past history of spontaneous miscarriage was not statistically different from the percentage of women without antibody and a past history of spontaneous abortion."}
{"id": "PMID:470747", "title": "\"Satellite\" haemodialysis.", "content": "Thirty of the 39 patients treated at the Blacktown Dialysis Centre, the first \"satellite\" dialysis centre in the greater metropolitan Sydney, had been referred from four Sydney renal units for long-term dialysis therapy. The move save approximately 150 kilometres in travelling and eight hours time each week for each of these patients. The cost of running the unit was approximately $10,000 per patient per year in the first year--no greater than that of home dialysis, and less than that of dialysis in a teaching hospital. The advantages of establishing satellite dialysis centre, the method of operation, and the results of the first year of operation of the Blacktown Dialysis Centre are described.", "contents": "\"Satellite\" haemodialysis. Thirty of the 39 patients treated at the Blacktown Dialysis Centre, the first \"satellite\" dialysis centre in the greater metropolitan Sydney, had been referred from four Sydney renal units for long-term dialysis therapy. The move save approximately 150 kilometres in travelling and eight hours time each week for each of these patients. The cost of running the unit was approximately $10,000 per patient per year in the first year--no greater than that of home dialysis, and less than that of dialysis in a teaching hospital. The advantages of establishing satellite dialysis centre, the method of operation, and the results of the first year of operation of the Blacktown Dialysis Centre are described."}
{"id": "PMID:470748", "title": "Cytodiagnosis of pancreatic and biliary cancer by endoscopic duct aspiration.", "content": "Duct aspiration was performed immediately after endoscopic retrograde cholangiopancreatography (ERCP) in 33 patients with ductal changes which were suggestive of pancreatic or biliary cancer. Malignant cells were identified in 10 of 17 patients (60%) with cancer of the pancreas, and in two of 10 patients (20%) with cancer of the biliary tract. Atypical cells were identified in additional patients with cancer, but cytological characteristics did not permit a definitive diagnosis. No false positive diagnoses were made. Duct aspiration is a relatively simple extension of ERCP, and enhances diagnostic accuracy, particularly in patients with carcinoma of the pancreas.", "contents": "Cytodiagnosis of pancreatic and biliary cancer by endoscopic duct aspiration. Duct aspiration was performed immediately after endoscopic retrograde cholangiopancreatography (ERCP) in 33 patients with ductal changes which were suggestive of pancreatic or biliary cancer. Malignant cells were identified in 10 of 17 patients (60%) with cancer of the pancreas, and in two of 10 patients (20%) with cancer of the biliary tract. Atypical cells were identified in additional patients with cancer, but cytological characteristics did not permit a definitive diagnosis. No false positive diagnoses were made. Duct aspiration is a relatively simple extension of ERCP, and enhances diagnostic accuracy, particularly in patients with carcinoma of the pancreas."}
{"id": "PMID:470749", "title": "Childhood deprivation: an antecedent of the irritable bowel syndrome.", "content": "Three hundred and thirty-three consecutive patients with irritable bowel syndrome (IBS) were interviewed. By the age of 15 years, 31% of the patients had either lost a parent through death, divorce or separation; 19% had an alcoholic parent, and 61% reported unsatisfactory relationships with, or between their parents. The findings support the important influence of childhood deprivation in the aetiology of IBS.", "contents": "Childhood deprivation: an antecedent of the irritable bowel syndrome. Three hundred and thirty-three consecutive patients with irritable bowel syndrome (IBS) were interviewed. By the age of 15 years, 31% of the patients had either lost a parent through death, divorce or separation; 19% had an alcoholic parent, and 61% reported unsatisfactory relationships with, or between their parents. The findings support the important influence of childhood deprivation in the aetiology of IBS."}
{"id": "PMID:470750", "title": "Prevalence of hepatitis B in a general hospital: screening of patients and staff.", "content": "Hepatitis B surface antigen (HBsAg) was sought over a six-month period in patients and staff members of the intensive care, cardiothoracic, and haematology/oncology units of a large general hospital. In addition, HBsAg was sought in all inpatients, outpatients and staff members at one point in time during August, 1977. A positive HBsAg state occurred with a prevalence of 1.55% in the three intensively studied units (cardiothoracic unit, 0.88%; haematology/oncology unit, 1.65%; and intensive care unit, 3.1%) and 0.9% for the point prevalence survey of hospital patients excluding the three intensively monitored areas. Very few of these patients had disorders traditionally associated with hepatitis B antigenaemia. In the survey of staff members, 0.72% of results were HBsAg positive. Of the factors examined, the most important determinant of the hepatitis B positive state both in patients and in staff members was their country of origin (that is, being born in southern or eastern Europe or in Asia).", "contents": "Prevalence of hepatitis B in a general hospital: screening of patients and staff. Hepatitis B surface antigen (HBsAg) was sought over a six-month period in patients and staff members of the intensive care, cardiothoracic, and haematology/oncology units of a large general hospital. In addition, HBsAg was sought in all inpatients, outpatients and staff members at one point in time during August, 1977. A positive HBsAg state occurred with a prevalence of 1.55% in the three intensively studied units (cardiothoracic unit, 0.88%; haematology/oncology unit, 1.65%; and intensive care unit, 3.1%) and 0.9% for the point prevalence survey of hospital patients excluding the three intensively monitored areas. Very few of these patients had disorders traditionally associated with hepatitis B antigenaemia. In the survey of staff members, 0.72% of results were HBsAg positive. Of the factors examined, the most important determinant of the hepatitis B positive state both in patients and in staff members was their country of origin (that is, being born in southern or eastern Europe or in Asia)."}
{"id": "PMID:470773", "title": "Evaluation of mebendazole for the treatment of trichuriasis.", "content": "Trichuris trichiura was found to cause severe refractory clinical illness in 25 child patients admitted to a rural hospital in northeast Queensland over a six-month period. Mebendazole produced significant alleviation of symptoms. Symptomless carrier states in 70 patients admitted to hospital for other complaints were also studied. Thirty-five of these were treated with mebendazole with a notable reduction in worm loads (as measured by Stoll egg counts) when compared with the control group. The effects of three-day and four-day courses of mebendazole on symptomless carriers are compared.", "contents": "Evaluation of mebendazole for the treatment of trichuriasis. Trichuris trichiura was found to cause severe refractory clinical illness in 25 child patients admitted to a rural hospital in northeast Queensland over a six-month period. Mebendazole produced significant alleviation of symptoms. Symptomless carrier states in 70 patients admitted to hospital for other complaints were also studied. Thirty-five of these were treated with mebendazole with a notable reduction in worm loads (as measured by Stoll egg counts) when compared with the control group. The effects of three-day and four-day courses of mebendazole on symptomless carriers are compared."}
{"id": "PMID:470774", "title": "Ultrasonography in an early hydatidiform mole.", "content": "An early hydatidiform mole containing a fetal sac is shown in grey scale echograms. It is suggested that all hydatidiform moles can be shown to contain a gestation sac if examined early enough.", "contents": "Ultrasonography in an early hydatidiform mole. An early hydatidiform mole containing a fetal sac is shown in grey scale echograms. It is suggested that all hydatidiform moles can be shown to contain a gestation sac if examined early enough."}
{"id": "PMID:470775", "title": "Severe hyperkalaemia with Moduretic.", "content": "Three patients with impaired renal function suffered complications of hyperkalaemia within 10 days of beginning therapy with hydrochlorothiazide and amiloride combination (Moduretic). The possible relationship between hyperkalaemia and this diuretic is discussed.", "contents": "Severe hyperkalaemia with Moduretic. Three patients with impaired renal function suffered complications of hyperkalaemia within 10 days of beginning therapy with hydrochlorothiazide and amiloride combination (Moduretic). The possible relationship between hyperkalaemia and this diuretic is discussed."}
{"id": "PMID:470776", "title": "High gastric ulcer after Nissen fundoplication.", "content": "Three of 100 patients who underwent Nissen fundoplication developed gastric ulcers, all high on the lesser curve. Two patients presented one year after, and the third seven years after fundoplication. All healed with medical therapy, but a relapse in one patient necessitated partial gastrectomy. The pathogenesis, is not known, but vascular factors may be relevant. Patients complaining of epigastric pain after fundoplication require careful endoscopic examination for ulcer, especially near the cardia, since this may be a site of predilection.", "contents": "High gastric ulcer after Nissen fundoplication. Three of 100 patients who underwent Nissen fundoplication developed gastric ulcers, all high on the lesser curve. Two patients presented one year after, and the third seven years after fundoplication. All healed with medical therapy, but a relapse in one patient necessitated partial gastrectomy. The pathogenesis, is not known, but vascular factors may be relevant. Patients complaining of epigastric pain after fundoplication require careful endoscopic examination for ulcer, especially near the cardia, since this may be a site of predilection."}
{"id": "PMID:470777", "title": "[Yersinia enterocolitica: A rather unknown agent (author's transl)].", "content": "A survey is given about epidemiological data and the different forms of clinical course of Yersiniosis enterocolitica. As a rule the enteric Yersiniosis heals spontaneoulsy without antibiotic therapy. In our own cases 9 of 13 patients were children. In the majority of these 13 cases symptoms of appendicitis were found. A survey about the behaviour against antibiotics of our tested strains show that Yersinia enterocolitica is resistant to all beta-lactam-antibiotics. The detection of Yersinia enterocolitica in stool specimens is not difficult provided that the inquiry is directed specially on this agent.", "contents": "[Yersinia enterocolitica: A rather unknown agent (author's transl)]. A survey is given about epidemiological data and the different forms of clinical course of Yersiniosis enterocolitica. As a rule the enteric Yersiniosis heals spontaneoulsy without antibiotic therapy. In our own cases 9 of 13 patients were children. In the majority of these 13 cases symptoms of appendicitis were found. A survey about the behaviour against antibiotics of our tested strains show that Yersinia enterocolitica is resistant to all beta-lactam-antibiotics. The detection of Yersinia enterocolitica in stool specimens is not difficult provided that the inquiry is directed specially on this agent."}
{"id": "PMID:470783", "title": "[Effect of biguanids on the lactate metabolism. Studies in diabetics under submaximal ergometric exercise (author's transl)].", "content": "24 diabetics without any complications were subjected to an exercise test before and after biguanid therapy. The schedule of the physical strain was a modification of the Kaltenbach diagram. Changes of lactate, pyruvate, pH-standard bicarbonate and base excess values were analysed. Additionally to antidiabetical therapy each of the 8 patients was given either metformin or buformin or phenformin now. Each one of the biguanid derivates induced a rise of the lactate level before and after the exercise test. Phenformin showed the greatest influence on the lactate metabolism, metformin the smallest. Reasons causing the lactogene effect and the quantitative differences of the biguanides are discussed.", "contents": "[Effect of biguanids on the lactate metabolism. Studies in diabetics under submaximal ergometric exercise (author's transl)]. 24 diabetics without any complications were subjected to an exercise test before and after biguanid therapy. The schedule of the physical strain was a modification of the Kaltenbach diagram. Changes of lactate, pyruvate, pH-standard bicarbonate and base excess values were analysed. Additionally to antidiabetical therapy each of the 8 patients was given either metformin or buformin or phenformin now. Each one of the biguanid derivates induced a rise of the lactate level before and after the exercise test. Phenformin showed the greatest influence on the lactate metabolism, metformin the smallest. Reasons causing the lactogene effect and the quantitative differences of the biguanides are discussed."}
{"id": "PMID:470784", "title": "[Correlation of glycerin-level and body weight (author's transl)].", "content": "In 202 non selected males and females the concentration of free glycerin in the fasting serum was evaluated. There was a significant parabolic correlation of the glycerin-levels in the serum  with the deviation of the actual body weight from the ideal weight. There is a theory that glycerin regulates the appetite through a negative feed-back mechanism. Our findings do not contradict this hypothesis, as it cannot be excluded that feedback mechanism is disturbed in long lasting obesity. The serum level of free glycerin is not correlated with the concentration of triglycerides or total cholesterol.", "contents": "[Correlation of glycerin-level and body weight (author's transl)]. In 202 non selected males and females the concentration of free glycerin in the fasting serum was evaluated. There was a significant parabolic correlation of the glycerin-levels in the serum  with the deviation of the actual body weight from the ideal weight. There is a theory that glycerin regulates the appetite through a negative feed-back mechanism. Our findings do not contradict this hypothesis, as it cannot be excluded that feedback mechanism is disturbed in long lasting obesity. The serum level of free glycerin is not correlated with the concentration of triglycerides or total cholesterol."}
{"id": "PMID:470785", "title": "[The effect of etofibrate on serum cholesterol and triglycerides (author's transl)].", "content": "Etofibrate retard administered in a daily dose 500 mg to 14 outpatients with hyperlipoproteinemia type IIa decreased the cholesterol only 1.7% after a treatment period of 6 weeks. After a treatment of 12 weeks the serum cholesterol was higher in 10 of the 14 patients than in the pretreatment period. Of low grade was the effect of etofibrate retard in 6 patients with type IIb or IV. The triglycerides decreased from 564 mg/dl to 455 mg/dl in the mean. The effect of this double ester from clofibrinic and nicotinic acid (59% to 41%) is uncertain in HLP type IIa, IIb and IV in the recommended dose of 500 mg/day.", "contents": "[The effect of etofibrate on serum cholesterol and triglycerides (author's transl)]. Etofibrate retard administered in a daily dose 500 mg to 14 outpatients with hyperlipoproteinemia type IIa decreased the cholesterol only 1.7% after a treatment period of 6 weeks. After a treatment of 12 weeks the serum cholesterol was higher in 10 of the 14 patients than in the pretreatment period. Of low grade was the effect of etofibrate retard in 6 patients with type IIb or IV. The triglycerides decreased from 564 mg/dl to 455 mg/dl in the mean. The effect of this double ester from clofibrinic and nicotinic acid (59% to 41%) is uncertain in HLP type IIa, IIb and IV in the recommended dose of 500 mg/day."}
{"id": "PMID:470786", "title": "[Endocrine insufficiency in alcaptonuria? A case report and survey of the literature (author's transl)].", "content": "In a 49 years old patient with alcaptonuria we studied the question whether disturbances of the endocrine organs by deposition of products of homogenistic acid could be detected analogous to disturbances of connective tissue. The function of the hypothalamus and the pituitary gland were shown to be normal. The only defect of the endocrine organs was a hypergonadotropic hypogonadism with a serum testosterone of 5.4 and 9.4 mumol/l respectively (155 and 270 ng/dl rsp.) at elevated LH and FSH values. A sufficient stimulation of the testosterone in serum by chorionic gonadotropin (Primogonyl) could be demonstrated, thus supporting the diagnosis of a compensated insuffiency of the Leydig cells. This disorder might be a late endocrine defect due to the basic metabolic disturbance of the disease.", "contents": "[Endocrine insufficiency in alcaptonuria? A case report and survey of the literature (author's transl)]. In a 49 years old patient with alcaptonuria we studied the question whether disturbances of the endocrine organs by deposition of products of homogenistic acid could be detected analogous to disturbances of connective tissue. The function of the hypothalamus and the pituitary gland were shown to be normal. The only defect of the endocrine organs was a hypergonadotropic hypogonadism with a serum testosterone of 5.4 and 9.4 mumol/l respectively (155 and 270 ng/dl rsp.) at elevated LH and FSH values. A sufficient stimulation of the testosterone in serum by chorionic gonadotropin (Primogonyl) could be demonstrated, thus supporting the diagnosis of a compensated insuffiency of the Leydig cells. This disorder might be a late endocrine defect due to the basic metabolic disturbance of the disease."}
{"id": "PMID:470788", "title": "[Pancreas gastricum intramurale submucosum in a 50-years old man (author's transl)].", "content": "Aberrant pancreas is a rare anomaly. It may be found by chance while operating or in autopsy and it need not cause any troubles to its bearer. In other cases it may be the cause of acute or chronic disorders. It is reported about a patient with chronic gastric disorders caused by heterotopic aberrant pancreas in the stomach wall.", "contents": "[Pancreas gastricum intramurale submucosum in a 50-years old man (author's transl)]. Aberrant pancreas is a rare anomaly. It may be found by chance while operating or in autopsy and it need not cause any troubles to its bearer. In other cases it may be the cause of acute or chronic disorders. It is reported about a patient with chronic gastric disorders caused by heterotopic aberrant pancreas in the stomach wall."}
{"id": "PMID:470789", "title": "[Lipoma and pseudolipoma of the liver (author's transl)].", "content": "Tumors and tumorlike lesions of fatty tissue arise only rarely in the liver. Those solitary nodules usually do not extend 2 cm in size and therefore remain clinically asymptomatic. Their gross appearance demands differential diagnosis from other tumorous or granulomatous lesions by means of biopsy and histological examination. Two characteristic cases with review of the literature are presented.", "contents": "[Lipoma and pseudolipoma of the liver (author's transl)]. Tumors and tumorlike lesions of fatty tissue arise only rarely in the liver. Those solitary nodules usually do not extend 2 cm in size and therefore remain clinically asymptomatic. Their gross appearance demands differential diagnosis from other tumorous or granulomatous lesions by means of biopsy and histological examination. Two characteristic cases with review of the literature are presented."}
{"id": "PMID:470791", "title": "[Treatment of highly resistant germs by dibekacin in patients with urinary tract infections (author's transl)].", "content": "The aminoglycoside dibekacin, developed in Japan and now on the market in Germany, has been tested for its clinical and microbiological effectiveness on 45 patients with highly resistant germs. 41 of the 55 primary sensitive germs could be thus eliminated from the urine, 6 of the 11 being pseudomonas germs. The clinical results of 33 of the 45 patients were very good, only 4 of them were not convincing. The nephrotoxic side effects were noticeably slight. No evidence of damage to the VIIIth brain nerve was observed in these patients. The results were satisfactory considering the selection of problem patients in the test group. Dibekacin can be regarded with gentamicin as a first choice aminoglycoside.", "contents": "[Treatment of highly resistant germs by dibekacin in patients with urinary tract infections (author's transl)]. The aminoglycoside dibekacin, developed in Japan and now on the market in Germany, has been tested for its clinical and microbiological effectiveness on 45 patients with highly resistant germs. 41 of the 55 primary sensitive germs could be thus eliminated from the urine, 6 of the 11 being pseudomonas germs. The clinical results of 33 of the 45 patients were very good, only 4 of them were not convincing. The nephrotoxic side effects were noticeably slight. No evidence of damage to the VIIIth brain nerve was observed in these patients. The results were satisfactory considering the selection of problem patients in the test group. Dibekacin can be regarded with gentamicin as a first choice aminoglycoside."}
{"id": "PMID:470792", "title": "[Treatment of hospital-acquired urinary infections with amikacin (author's transl)].", "content": "The efficacy of amikacin was evaluated in 32 patients with urinary infections due to hospital-acquired rods. Treatment with amikacin was successful in all cases of acute urinary infection by gentamicin-resistent gram-negative strains. Amikacin shares with other aminoglycosides the risk of nephrotoxicity and ototoxicity. Follow-up studies are preferable to patients already suffering from damage of hearing. Lesions of n. vestibularis were relatively infrequent.", "contents": "[Treatment of hospital-acquired urinary infections with amikacin (author's transl)]. The efficacy of amikacin was evaluated in 32 patients with urinary infections due to hospital-acquired rods. Treatment with amikacin was successful in all cases of acute urinary infection by gentamicin-resistent gram-negative strains. Amikacin shares with other aminoglycosides the risk of nephrotoxicity and ototoxicity. Follow-up studies are preferable to patients already suffering from damage of hearing. Lesions of n. vestibularis were relatively infrequent."}
{"id": "PMID:470798", "title": "[The influence of age and weight on the normal oral glucose tolerance (author's transl)].", "content": "The individual influence of age and weight on the normal glucose tolerance after an oral glucose load of 50 g was investigated in 332 test subjects (231 male + 101 female) presenting a healthy metabolism. The results show that, even within the range of normal glucose tolerance, the relative weight (age factor eliminated) exerts an influence both on the fasting blood sugar and on the 1 h-value (p less than or equal to 0.01), in women even on the 2 h-value (p less than or equal to 0.05) (\"plain\" glucose tolerance--weight correlation). The influence of the age factor (weight eliminated) on the normal glucose tolerance can only be demonstrated in the 1 h-value of men as sole value (p less than or equal to 0.01). Here it even slightly exceeds the influence of the weight factor (\"plain\" glucose tolerance--age correlation). Apart from this value, however, it can be stated that, within the normal glucose tolerance of both male and female subjects, the weight factor is of greater importance than the age factor. With increasing weight the sugar levels progressively approach the upper limit of the norm. Perhaps this is already the forefield to future secondary arteriopathies.", "contents": "[The influence of age and weight on the normal oral glucose tolerance (author's transl)]. The individual influence of age and weight on the normal glucose tolerance after an oral glucose load of 50 g was investigated in 332 test subjects (231 male + 101 female) presenting a healthy metabolism. The results show that, even within the range of normal glucose tolerance, the relative weight (age factor eliminated) exerts an influence both on the fasting blood sugar and on the 1 h-value (p less than or equal to 0.01), in women even on the 2 h-value (p less than or equal to 0.05) (\"plain\" glucose tolerance--weight correlation). The influence of the age factor (weight eliminated) on the normal glucose tolerance can only be demonstrated in the 1 h-value of men as sole value (p less than or equal to 0.01). Here it even slightly exceeds the influence of the weight factor (\"plain\" glucose tolerance--age correlation). Apart from this value, however, it can be stated that, within the normal glucose tolerance of both male and female subjects, the weight factor is of greater importance than the age factor. With increasing weight the sugar levels progressively approach the upper limit of the norm. Perhaps this is already the forefield to future secondary arteriopathies."}
{"id": "PMID:470835", "title": "Use of casein, tyrosine, and hypoxanthine in the identification of nonfermentative gram-negative bacilli.", "content": "Four hundred and five (405) strains of nonfermentative gram-negative bacilli (NFB) were identified using conventional tests. The NFB studied include 323 originating from clinical specimens, 37 from environmental samples, and 45 authentic strains from other workers and from our culture collections. All these strains were studied for their ability to degradate casein, tyrosine, and hypoxanthine. It was found that these tests are useful in identifying NFB within 24 to 48 h when used with other tests.", "contents": "Use of casein, tyrosine, and hypoxanthine in the identification of nonfermentative gram-negative bacilli. Four hundred and five (405) strains of nonfermentative gram-negative bacilli (NFB) were identified using conventional tests. The NFB studied include 323 originating from clinical specimens, 37 from environmental samples, and 45 authentic strains from other workers and from our culture collections. All these strains were studied for their ability to degradate casein, tyrosine, and hypoxanthine. It was found that these tests are useful in identifying NFB within 24 to 48 h when used with other tests."}
{"id": "PMID:470836", "title": "Application of the laser to the study of pathogenic fungi.", "content": "Laser microanalysis has been applied to the study of pathogenic fungi. Such a method allows chemical information to be obtained and permits the detection of 74 elements in the periodic system of Mendeleev from lithium (3) to uranium (92).", "contents": "Application of the laser to the study of pathogenic fungi. Laser microanalysis has been applied to the study of pathogenic fungi. Such a method allows chemical information to be obtained and permits the detection of 74 elements in the periodic system of Mendeleev from lithium (3) to uranium (92)."}
{"id": "PMID:470837", "title": "Naked megakaryocyte nuclei revisited.", "content": "Bone marrow smears from 35 patients with various neoplasms and from 32 control patients were examined for the presence and percentage of naked megakaryocyte nuclei (NMN). The percentages of NMN in the two groups did not significantly differ. We conclude that the enumeration of NMN is of no value as a diagnostic test of malignancy.", "contents": "Naked megakaryocyte nuclei revisited. Bone marrow smears from 35 patients with various neoplasms and from 32 control patients were examined for the presence and percentage of naked megakaryocyte nuclei (NMN). The percentages of NMN in the two groups did not significantly differ. We conclude that the enumeration of NMN is of no value as a diagnostic test of malignancy."}
{"id": "PMID:470838", "title": "Teratomas in children and young adults.", "content": "A review of all teratomas seen at the University of Wisconsin Hospital between 1965 and 1977 revealed that sacrococcygeal and presacral teratomas were most common. In these cases survival was best in infants less than one year old, with the exception of two cases of malignant medulloepithelioma. Testicular teratomas were predominant in the young adult male, and survival was poor. Pathology and treatment of teratomas are discussed, with an accompanying discussion of congenital anomalies associated with teratomas.", "contents": "Teratomas in children and young adults. A review of all teratomas seen at the University of Wisconsin Hospital between 1965 and 1977 revealed that sacrococcygeal and presacral teratomas were most common. In these cases survival was best in infants less than one year old, with the exception of two cases of malignant medulloepithelioma. Testicular teratomas were predominant in the young adult male, and survival was poor. Pathology and treatment of teratomas are discussed, with an accompanying discussion of congenital anomalies associated with teratomas."}
{"id": "PMID:470839", "title": "Childhood non-Hodgkin Malignant lymphomas: a clinicopathologic retrospective study.", "content": "Between 1968 and 1975, 44 evaluable children under 16 years of age with the histologic diagnosis of non-Hodgkin malignant lymphoma (ML) were treated at the Istituto Nazionale Tumori of Milan. Histologic diagnoses were reclassified as follows: 13 lymphoblastic (others) ML, 15 convoluted cell type lymphoblastic ML, 9 Burkitt type ML, and 7 immunoblastic ML. Only 36% of the patients had stage I and II disease. At diagnosis 25% showed malignant cells in the bone marrow smears. Bone marrow infiltration was particularly frequent in the convoluted cell type lymphoblastic ML and in the lymphoblastic (others) ML subgroups. Burkitt type ML frequently was associated with abdominal lesions and subsequently a high incidence of central nervous system involvement. Patients with stage I and II ML were encountered mostly in the immunoblastic ML subgroup. After 1973 more intensive chemotherapy plus radiotherapy seems to have slightly improved the survival of the patients, except in the Burkitt type ML Subgroup.", "contents": "Childhood non-Hodgkin Malignant lymphomas: a clinicopathologic retrospective study. Between 1968 and 1975, 44 evaluable children under 16 years of age with the histologic diagnosis of non-Hodgkin malignant lymphoma (ML) were treated at the Istituto Nazionale Tumori of Milan. Histologic diagnoses were reclassified as follows: 13 lymphoblastic (others) ML, 15 convoluted cell type lymphoblastic ML, 9 Burkitt type ML, and 7 immunoblastic ML. Only 36% of the patients had stage I and II disease. At diagnosis 25% showed malignant cells in the bone marrow smears. Bone marrow infiltration was particularly frequent in the convoluted cell type lymphoblastic ML and in the lymphoblastic (others) ML subgroups. Burkitt type ML frequently was associated with abdominal lesions and subsequently a high incidence of central nervous system involvement. Patients with stage I and II ML were encountered mostly in the immunoblastic ML subgroup. After 1973 more intensive chemotherapy plus radiotherapy seems to have slightly improved the survival of the patients, except in the Burkitt type ML Subgroup."}
{"id": "PMID:470840", "title": "Pulmonary toxicity from carmustine (BCNU): a case report.", "content": "A patient who had a pneumonectomy for lung carcinoma was treated with carmustine when brain metastases developed. His pulmonary function was mildly compromised prior to the pneumonectomy by many years of smoking. After six months of carmustine therapy [total dose: 2,250 mg (1,200 mg/m2)] he developed interstitial pulmonary fibrosis with histologic changes consistent with drug toxicity. With seven previously reported cases of this drug-effect and the addition of our case, carmustine must be added to the list of cancer chemotherapeutic agents that can cause pulmonary toxicity.", "contents": "Pulmonary toxicity from carmustine (BCNU): a case report. A patient who had a pneumonectomy for lung carcinoma was treated with carmustine when brain metastases developed. His pulmonary function was mildly compromised prior to the pneumonectomy by many years of smoking. After six months of carmustine therapy [total dose: 2,250 mg (1,200 mg/m2)] he developed interstitial pulmonary fibrosis with histologic changes consistent with drug toxicity. With seven previously reported cases of this drug-effect and the addition of our case, carmustine must be added to the list of cancer chemotherapeutic agents that can cause pulmonary toxicity."}
{"id": "PMID:470841", "title": "A feasibility study of prompt capture gamma in vivo neutron activation analysis.", "content": "The feasibility of using the information contained in the radiative capture gamma ray spectrum of the neutron-irradiated human body to measure quantitatively total body elemental composition in vivo has been investigated. Results of time dependent Monte Carlo simulations have shown that spectral interference of nonradiative capture origin can be completely eliminated by pulsing the detector/spectrometer system in anticoincidence with the neutron source. Calculations based on the results of the Monte Carlo simulation and on an experimental measurement of the efficiency versus energy characteristics of a Ge(Li) detector suggest that the primary limitation of the proposed technique would be inter-element spectral interference rather than inadequate detector sensitivity. Experimental measurements using a pulsed 14-MeV neutron generator and Ge(Li) gamma-ray spectrometer have produced results that are consistent with the predictions of the theoretical model. A radiative capture gamma-ray spectrum of a tissue-equivalent phantom was measured in pulsed mode and analyzed offline using a computerized spectral analysis procedure. The results were scaled to a proposed facility consisting of a 2.5-MeV pulsed neutron source and a detection system comprising two 50-cm3 (Ge(Li) detectros past which the subject would be scanned. It has been shown that in principle the elements hydrogen, chlorine, calcium, and nitrogen [the latter using large NaI(T1) detectors] could be measured with such a facility at an average body dose level of 0.1 rad. At this dose level the coefficients of variation based on counting statistics alone would be +/- 2%, or better, for these four elements. With an improvement in the detector/spectrometer energy resolution, the elements sulfur and carbon might also be measurable. It is also shown that by modifying the pulsing sequence appropriately and using 14-MeV neutrons, total body oxygen could also be measured at the 0.1 rad dose level via its inelastic neutron scattering deexcitation gamma activity.", "contents": "A feasibility study of prompt capture gamma in vivo neutron activation analysis. The feasibility of using the information contained in the radiative capture gamma ray spectrum of the neutron-irradiated human body to measure quantitatively total body elemental composition in vivo has been investigated. Results of time dependent Monte Carlo simulations have shown that spectral interference of nonradiative capture origin can be completely eliminated by pulsing the detector/spectrometer system in anticoincidence with the neutron source. Calculations based on the results of the Monte Carlo simulation and on an experimental measurement of the efficiency versus energy characteristics of a Ge(Li) detector suggest that the primary limitation of the proposed technique would be inter-element spectral interference rather than inadequate detector sensitivity. Experimental measurements using a pulsed 14-MeV neutron generator and Ge(Li) gamma-ray spectrometer have produced results that are consistent with the predictions of the theoretical model. A radiative capture gamma-ray spectrum of a tissue-equivalent phantom was measured in pulsed mode and analyzed offline using a computerized spectral analysis procedure. The results were scaled to a proposed facility consisting of a 2.5-MeV pulsed neutron source and a detection system comprising two 50-cm3 (Ge(Li) detectros past which the subject would be scanned. It has been shown that in principle the elements hydrogen, chlorine, calcium, and nitrogen [the latter using large NaI(T1) detectors] could be measured with such a facility at an average body dose level of 0.1 rad. At this dose level the coefficients of variation based on counting statistics alone would be +/- 2%, or better, for these four elements. With an improvement in the detector/spectrometer energy resolution, the elements sulfur and carbon might also be measurable. It is also shown that by modifying the pulsing sequence appropriately and using 14-MeV neutrons, total body oxygen could also be measured at the 0.1 rad dose level via its inelastic neutron scattering deexcitation gamma activity."}
{"id": "PMID:470842", "title": "The design and performance of a scanning multiple slit assembly.", "content": "A scanning multiple slit assembly (SMSA) has been constructed for the purpose of reducing scatter in medical radiography. The SMSA consists of a series of long, narrow beam-defining slits above the patient that are aligned and synchronously moved with scatter-eliminating slots beneath the patient during an exposure. Evidence, based on measurements of the ratio of scattered-to-primary radiation imaged and radiographs of patients, is presented indicating that such a device is a practical and efficient method of reducing scatter and improving contrast compared to conventional grids. The design considerations and trade-offs associated with the choice of slit width, slit separation distance, and aft slot depth are discussed along with the effect of these parameters on the SMSA's performance. The various problems encountered in obtaining a uniform scan and the manner in which they were handled are also discussed.", "contents": "The design and performance of a scanning multiple slit assembly. A scanning multiple slit assembly (SMSA) has been constructed for the purpose of reducing scatter in medical radiography. The SMSA consists of a series of long, narrow beam-defining slits above the patient that are aligned and synchronously moved with scatter-eliminating slots beneath the patient during an exposure. Evidence, based on measurements of the ratio of scattered-to-primary radiation imaged and radiographs of patients, is presented indicating that such a device is a practical and efficient method of reducing scatter and improving contrast compared to conventional grids. The design considerations and trade-offs associated with the choice of slit width, slit separation distance, and aft slot depth are discussed along with the effect of these parameters on the SMSA's performance. The various problems encountered in obtaining a uniform scan and the manner in which they were handled are also discussed."}
{"id": "PMID:470843", "title": "Relative carcinogenic effects of different mammography techniques.", "content": "Comparisons of different mammography techniques can be significantly altered by assumptions about the relevant measures for carcinogenic effects in mammography at low doses. Using a combination of recent data available from the literature, a study as been made of the significance of various assumptions concerning both the appropriate measures for carcinogenesis in mammography and the shape of the dose-effects relationship. It is found that for techniques typical of current reduced-dose mammography systems, the comparison of the relative carcinogenic effects of the techniques is significantly affected by both the measures that are used and the shape of th dose-effects relationship.", "contents": "Relative carcinogenic effects of different mammography techniques. Comparisons of different mammography techniques can be significantly altered by assumptions about the relevant measures for carcinogenic effects in mammography at low doses. Using a combination of recent data available from the literature, a study as been made of the significance of various assumptions concerning both the appropriate measures for carcinogenesis in mammography and the shape of the dose-effects relationship. It is found that for techniques typical of current reduced-dose mammography systems, the comparison of the relative carcinogenic effects of the techniques is significantly affected by both the measures that are used and the shape of th dose-effects relationship."}
{"id": "PMID:470844", "title": "A laser scanner for the densitometric analysis of radiographs.", "content": "An instrument is described which can scan radiographs and collect densitometric readings for subsequent computer analysis. A laser beam is focused onto the film. Raster scan of the beam is provided by two orthogonal mirror galvanometers. The light, attenuated by the radiograph, is gathered by a lens in such a way that it is directed into a small detector regardless of the scan position. Beam position signals and density readings are stored and analyzed in a minicomputer.", "contents": "A laser scanner for the densitometric analysis of radiographs. An instrument is described which can scan radiographs and collect densitometric readings for subsequent computer analysis. A laser beam is focused onto the film. Raster scan of the beam is provided by two orthogonal mirror galvanometers. The light, attenuated by the radiograph, is gathered by a lens in such a way that it is directed into a small detector regardless of the scan position. Beam position signals and density readings are stored and analyzed in a minicomputer."}
{"id": "PMID:470846", "title": "Absorbed dose comparison: positron emitters 11C, 13N, and 15O versus gamma-ray emitters.", "content": "Absorbed doses were calculated or taken from the literature for various compounds of 11C, 13N, and 15O, and compared to those of presently gamma-ray-emitting nuclear medicine radiopharmaceuticals. As a rule of thumb, the doses per millicurie of the injectable positron-emitting compounds are of the same order of magnitude as the dose per millicurie of 99mTc compounds. The absorbed doses from the injectable positron emitters are nearly one or more orders of magnitude lower on a per millicurie basis than those from the other injectable gamma emitters that were investigated.", "contents": "Absorbed dose comparison: positron emitters 11C, 13N, and 15O versus gamma-ray emitters. Absorbed doses were calculated or taken from the literature for various compounds of 11C, 13N, and 15O, and compared to those of presently gamma-ray-emitting nuclear medicine radiopharmaceuticals. As a rule of thumb, the doses per millicurie of the injectable positron-emitting compounds are of the same order of magnitude as the dose per millicurie of 99mTc compounds. The absorbed doses from the injectable positron emitters are nearly one or more orders of magnitude lower on a per millicurie basis than those from the other injectable gamma emitters that were investigated."}
{"id": "PMID:470847", "title": "Multiple window rectilinear scanner modification.", "content": "Although the gamma camera and multi-crystal scanner have largely replaced the old dual probe rectlinear scanners in the larger hospitals, a large number of such scanners are still in use today. Modifications to these units can greatly enhance their usefulness. This paper describes the addition of dual SCA windows to each probe of a rectlinear scanner to increase sensitivity to multiple gamma-ray isotopes such as 67Ga. This method allows increased information density and/or decreased scanning time without degrading image quality. NIM electronics are used to keep down capital costs, as these modules can be shared with other counting systems when multiple-peak isotopes are not being scanned. A simple connection to an Ohio Nuclear Mdl 84 dual probe scanner is described. For other scanners, a slight modification would be necessary.", "contents": "Multiple window rectilinear scanner modification. Although the gamma camera and multi-crystal scanner have largely replaced the old dual probe rectlinear scanners in the larger hospitals, a large number of such scanners are still in use today. Modifications to these units can greatly enhance their usefulness. This paper describes the addition of dual SCA windows to each probe of a rectlinear scanner to increase sensitivity to multiple gamma-ray isotopes such as 67Ga. This method allows increased information density and/or decreased scanning time without degrading image quality. NIM electronics are used to keep down capital costs, as these modules can be shared with other counting systems when multiple-peak isotopes are not being scanned. A simple connection to an Ohio Nuclear Mdl 84 dual probe scanner is described. For other scanners, a slight modification would be necessary."}
{"id": "PMID:470848", "title": "Evaluation of a new x-ray film with reduced crossover.", "content": "The improvement in MTF that can be obtained by the reduction of crossover exposures is evaluated using an experimental film supplied by the 3M Company (3M XUD). It was found that image resolution, as measured by the equivalent passband (2 integral of infinity 0 [M(f)]2df), of 3M Trimax 8 screens when used with the experimental 3M XUD film was 2.9 c/mm as compared to 1.8 c/mm when used with a conventional double coated film such as Kodak Ortho G or 3M XD. On the other hand, system speed with 3M XUD film was only 1.6 mR-1 as compared to 3.2 mR-1 with 3M XD film and 2.4 mR-1 with Kodak Ortho G film. These findings were substantiated by phantom studies as well.", "contents": "Evaluation of a new x-ray film with reduced crossover. The improvement in MTF that can be obtained by the reduction of crossover exposures is evaluated using an experimental film supplied by the 3M Company (3M XUD). It was found that image resolution, as measured by the equivalent passband (2 integral of infinity 0 [M(f)]2df), of 3M Trimax 8 screens when used with the experimental 3M XUD film was 2.9 c/mm as compared to 1.8 c/mm when used with a conventional double coated film such as Kodak Ortho G or 3M XD. On the other hand, system speed with 3M XUD film was only 1.6 mR-1 as compared to 3.2 mR-1 with 3M XD film and 2.4 mR-1 with Kodak Ortho G film. These findings were substantiated by phantom studies as well."}
{"id": "PMID:470849", "title": "Effects of focal spot intensity distribution and collimator width in reconstructive x-ray tomography.", "content": "In computed tomography the finite width of the collimator aperture implies that the image is reconstructed from scans having finite strip width. This blurs the image and limits scanner resolution. Bracewell has discussed the nature of the blurring produced and suggested a method to partially restore the image under the assumption that the x-ray focal spot has a uniform intensity distribution. However, pinhole radiographs of x-ray focal spots reveal intensity distribution in the scanning plane which peaks at the edges of the focal spot with a reduced intensity in the center. A model is presented which simulates this intensity distribution as the sum of two point sources of x rays separated by a uniform line source. This results in a point absorber response function which is a superposition of two rectangular distributions and the one discussed by Bracewell. The corresponding tranfer function is derived which shows that the intensity distribution of the focal spot influences the high spatial frequency components of the reconstructed image more than the low frequency components.", "contents": "Effects of focal spot intensity distribution and collimator width in reconstructive x-ray tomography. In computed tomography the finite width of the collimator aperture implies that the image is reconstructed from scans having finite strip width. This blurs the image and limits scanner resolution. Bracewell has discussed the nature of the blurring produced and suggested a method to partially restore the image under the assumption that the x-ray focal spot has a uniform intensity distribution. However, pinhole radiographs of x-ray focal spots reveal intensity distribution in the scanning plane which peaks at the edges of the focal spot with a reduced intensity in the center. A model is presented which simulates this intensity distribution as the sum of two point sources of x rays separated by a uniform line source. This results in a point absorber response function which is a superposition of two rectangular distributions and the one discussed by Bracewell. The corresponding tranfer function is derived which shows that the intensity distribution of the focal spot influences the high spatial frequency components of the reconstructed image more than the low frequency components."}
{"id": "PMID:470850", "title": "Gamma dose measurement in a d(80) + Be neutron beam.", "content": "The gamma-ray dose component of a d(80) + Be neutron beam was measured with a 6Li-shielded Geiger-Mueller counter in a tissue-equivalent liquid phantom. The proportion of gamma-ray dose to total dose increased from 0.07 near the surface to 0.09 at 25 cm depth. Due to thermal neutron sensitivity, the G-M count rate increased by 25% in the absence of 6Li shielding.", "contents": "Gamma dose measurement in a d(80) + Be neutron beam. The gamma-ray dose component of a d(80) + Be neutron beam was measured with a 6Li-shielded Geiger-Mueller counter in a tissue-equivalent liquid phantom. The proportion of gamma-ray dose to total dose increased from 0.07 near the surface to 0.09 at 25 cm depth. Due to thermal neutron sensitivity, the G-M count rate increased by 25% in the absence of 6Li shielding."}
{"id": "PMID:470936", "title": "[Metabolically stable classes of messenger-like nuclear RNA. II. Existence of homology between the 28S fraction of nuclear RNA, giant non-polyadenylated molecules of heterogeneous nuclear RNA and polyadenylated molecules of cytoplasmic RNA].", "content": "The existence and extent of homology between the 28S fraction of messenger-like nuclear RNA on one hand, and giant (greater than 45S) molecules of heterogeneous nuclear RNA and various fractions of cytoplasmic RNA on the other hand were studied by the method of competitive hybridization. The results obtained lead to the conclusion that the poly(A)-subfraction of hnRNA molecules with the size of greater than 15 000 nucleotides contains molecules subjected to rapid but incomplete processing with the formation of chains approximately 5000 nucleotides long (approximately 28S), which are then stored in the nucleus for up to 30 hours. Later polyadenylated and non-polyadenylated molecules of cytoplasmic RNA with the size of less than or equal to 2000 nucleotides are formed from the stored precursors.", "contents": "[Metabolically stable classes of messenger-like nuclear RNA. II. Existence of homology between the 28S fraction of nuclear RNA, giant non-polyadenylated molecules of heterogeneous nuclear RNA and polyadenylated molecules of cytoplasmic RNA]. The existence and extent of homology between the 28S fraction of messenger-like nuclear RNA on one hand, and giant (greater than 45S) molecules of heterogeneous nuclear RNA and various fractions of cytoplasmic RNA on the other hand were studied by the method of competitive hybridization. The results obtained lead to the conclusion that the poly(A)-subfraction of hnRNA molecules with the size of greater than 15 000 nucleotides contains molecules subjected to rapid but incomplete processing with the formation of chains approximately 5000 nucleotides long (approximately 28S), which are then stored in the nucleus for up to 30 hours. Later polyadenylated and non-polyadenylated molecules of cytoplasmic RNA with the size of less than or equal to 2000 nucleotides are formed from the stored precursors."}
{"id": "PMID:470937", "title": "[Model for tobacco mosaic virus assembly in vitro: specific dislocation by cooperative single-letter purine recognition and non-cooperative RNA \"locking\" between subunit layers].", "content": "A model of the molecular mechanism of tobacco mosaic virus (TMV) assembly in vitro is proposed. The model is based on the assumption that on interaction of TMV RNA chain with a double protein disk two different types of structural alterations occur in the protein subunits: \"dislocating alterations\" leading to screw dislocation of the disk into a helix fragment and \"locking alterations\" leading to locking of the RNA chain between the subunits layers. During initiation of TMV assembly the dislocating alterations occur cooperatively in all subunits of the disk as a result of specific interaction of purines (guanines) in the middle (horizontal) position of every trinucleotide unit with the 32--39 loop of the TMV protein molecule. Locking alterations are induced non-cooperatively by phosphate groups and lateral (vertical) bases of trinucleotide units. The sequence of events during TMV assembly in vitro and the role of topological factors in this process are discussed.", "contents": "[Model for tobacco mosaic virus assembly in vitro: specific dislocation by cooperative single-letter purine recognition and non-cooperative RNA \"locking\" between subunit layers]. A model of the molecular mechanism of tobacco mosaic virus (TMV) assembly in vitro is proposed. The model is based on the assumption that on interaction of TMV RNA chain with a double protein disk two different types of structural alterations occur in the protein subunits: \"dislocating alterations\" leading to screw dislocation of the disk into a helix fragment and \"locking alterations\" leading to locking of the RNA chain between the subunits layers. During initiation of TMV assembly the dislocating alterations occur cooperatively in all subunits of the disk as a result of specific interaction of purines (guanines) in the middle (horizontal) position of every trinucleotide unit with the 32--39 loop of the TMV protein molecule. Locking alterations are induced non-cooperatively by phosphate groups and lateral (vertical) bases of trinucleotide units. The sequence of events during TMV assembly in vitro and the role of topological factors in this process are discussed."}
{"id": "PMID:470938", "title": "[Conditions of formation of DNA optically-active particles in polyethyleneglycol-containing solutions of different salts].", "content": "Conditions of formation of the DNA optically-active compact particles (e. g. particles which are characterized by intense negative band in CD spectrum) in PEG-containing solutions of NaCl, NaClO4, KCl, KBr, KI and CsCL have been studied. It has been shown that the region of existence of the DNA optically-active compact particles is restricted not only by the definite concentration of PEG, but also by the definite ionic strength of solution. Above this region the intense band in CD spectra of the DNA compact particles is practically absent. The nature of cation influences the process of compactization (condensation) of the DNA double-stranded molecules; the nature of anion does not influence this process (up to 0,3 M salt concentration).", "contents": "[Conditions of formation of DNA optically-active particles in polyethyleneglycol-containing solutions of different salts]. Conditions of formation of the DNA optically-active compact particles (e. g. particles which are characterized by intense negative band in CD spectrum) in PEG-containing solutions of NaCl, NaClO4, KCl, KBr, KI and CsCL have been studied. It has been shown that the region of existence of the DNA optically-active compact particles is restricted not only by the definite concentration of PEG, but also by the definite ionic strength of solution. Above this region the intense band in CD spectra of the DNA compact particles is practically absent. The nature of cation influences the process of compactization (condensation) of the DNA double-stranded molecules; the nature of anion does not influence this process (up to 0,3 M salt concentration)."}
{"id": "PMID:470939", "title": "[Molecular mechanisms of 2-aminopurine and 2,6-diaminopurine induced mutations].", "content": "Molecular mechanisms of base pair substitutions induced by 2-aminopurine and 2,6-diaminopurine have been proposed by calculations of nonbonded interaction energy of this substances with nucleic acid bases. The calculation results suppose that the main pathways of the transitions are wobble pair formations of these analogues with cytosine. The possibility of the transversions as a result of incorporation of 2-aminopurine and 2,6-diaminopurine in DNA have been considered. The influence of nucleotide sequences and enzymes of nucleic acid synthesis on induced mutation frequencies have been discussed.", "contents": "[Molecular mechanisms of 2-aminopurine and 2,6-diaminopurine induced mutations]. Molecular mechanisms of base pair substitutions induced by 2-aminopurine and 2,6-diaminopurine have been proposed by calculations of nonbonded interaction energy of this substances with nucleic acid bases. The calculation results suppose that the main pathways of the transitions are wobble pair formations of these analogues with cytosine. The possibility of the transversions as a result of incorporation of 2-aminopurine and 2,6-diaminopurine in DNA have been considered. The influence of nucleotide sequences and enzymes of nucleic acid synthesis on induced mutation frequencies have been discussed."}
{"id": "PMID:470940", "title": "[Structural studies on tubulin in flat sheets formed in the presence of zinc].", "content": "Structure of the tubulin molecule was determined from microtubule images by methods of three dimensional reconstruction. We detected two types of zinc-induced sheets with different space latticies. Using the model of the tubulin molecule obtained by three dimensional reconstruction, the relationship of its projections was established by the method of optical filtration. Structural peculiarities in tubulin polymorphism are considered.", "contents": "[Structural studies on tubulin in flat sheets formed in the presence of zinc]. Structure of the tubulin molecule was determined from microtubule images by methods of three dimensional reconstruction. We detected two types of zinc-induced sheets with different space latticies. Using the model of the tubulin molecule obtained by three dimensional reconstruction, the relationship of its projections was established by the method of optical filtration. Structural peculiarities in tubulin polymorphism are considered."}
{"id": "PMID:470941", "title": "[Investigation of the initial steps of protochlorophyllide photoreduction in etiolated plants].", "content": "The irreversible nonfluorescent product of the protochlorophyllide photoreduction with the wide electronic band at 705 nm was discovered. The P705 product through the intermediate products with the fluorescence max at 695 nm and 687 nm turns into Chl684 678, as the result of heating in darkness. The P705, P695, P687 products quench protochlorophyllide fluorescence with great efficiency. Analysis of the intermediate product properties allow to propose a new photoreduction mechanism that includes energy transfer to the trapping center where the charge transfer is performed, nonradiative energy dissipation in the charge-transfer complex, and protonation and disproportionation of the pigment anion-radicals.", "contents": "[Investigation of the initial steps of protochlorophyllide photoreduction in etiolated plants]. The irreversible nonfluorescent product of the protochlorophyllide photoreduction with the wide electronic band at 705 nm was discovered. The P705 product through the intermediate products with the fluorescence max at 695 nm and 687 nm turns into Chl684 678, as the result of heating in darkness. The P705, P695, P687 products quench protochlorophyllide fluorescence with great efficiency. Analysis of the intermediate product properties allow to propose a new photoreduction mechanism that includes energy transfer to the trapping center where the charge transfer is performed, nonradiative energy dissipation in the charge-transfer complex, and protonation and disproportionation of the pigment anion-radicals."}
{"id": "PMID:470944", "title": "[Mixed aggregation of pigments with participation of bacteriochlorophyll].", "content": "In binar mixture of solvents (dioxane--water, 1 : 4) the spectral-luminescent investigation of the regularities of mixed association and the energetic interaction between molecules of bacteriochlorophyll (BChl) and its analogs (chlorophyll a--Chl, protochlorophyll--PChl, 4-vinyl-protochlorophyll--4VPChl) under variation of donor-acceptor ratio (0,001 less than or equal to CD/CA less than or equal to 1) in the polymer complex was conducted. The essential role of the elementary cell structure of the mixed associate was discovered: the associate of individual BChl did not possess measurable fluorescence whereas the introduction of PChl, 4VPChl and Chl admixture (approximately greater than 1%) into BChl aggregate resulted in the appearance of a BChl fluorescent ability. It was suggested that admixture and BChl molecules formed dimer cells in which the excitation nonradiative deactivation probability was strongly decreased in comparison with individual BChl aggregate. These cells are the centres of mixed associates emission. It was shown that under conditions of effective energy migration in the system of nearly arranged centres with a high degree of orientation the excitation trapping probability strongly depended on the resonance conditions for transition density--transition density interaction between partners of aggregation.", "contents": "[Mixed aggregation of pigments with participation of bacteriochlorophyll]. In binar mixture of solvents (dioxane--water, 1 : 4) the spectral-luminescent investigation of the regularities of mixed association and the energetic interaction between molecules of bacteriochlorophyll (BChl) and its analogs (chlorophyll a--Chl, protochlorophyll--PChl, 4-vinyl-protochlorophyll--4VPChl) under variation of donor-acceptor ratio (0,001 less than or equal to CD/CA less than or equal to 1) in the polymer complex was conducted. The essential role of the elementary cell structure of the mixed associate was discovered: the associate of individual BChl did not possess measurable fluorescence whereas the introduction of PChl, 4VPChl and Chl admixture (approximately greater than 1%) into BChl aggregate resulted in the appearance of a BChl fluorescent ability. It was suggested that admixture and BChl molecules formed dimer cells in which the excitation nonradiative deactivation probability was strongly decreased in comparison with individual BChl aggregate. These cells are the centres of mixed associates emission. It was shown that under conditions of effective energy migration in the system of nearly arranged centres with a high degree of orientation the excitation trapping probability strongly depended on the resonance conditions for transition density--transition density interaction between partners of aggregation."}
{"id": "PMID:470943", "title": "[Conformation of nucleotides, oligonucleotides and their analogues in aqueous solution. II. Syn-anti-equilibrium in solutions of adenosine, 5'-AMP, 3'-AMP, 5'-CMP and 3'-CMP].", "content": "A method for determining the equilibrium constant of sin-anti-states in the aqueous solution of purine and pyrimidine nucleotides and nucleosides has been proposed. This method is based on the measurement of the spin-lattice relaxation rate of H(1') atom of purine nucleotides before and after exchange of H(8) deuterium in the case of purine nucleotides or H(1') and H(5) atoms of pyrimidine nucleotides. The results obtained were interpreted by the two-state dynamic model. The method applied for investigation of the conformation situation in solutions of Ado, 5'-AMP, 3'-AMP, 5'-CMP and 3'-CMP. 5'-AMP and 5'-CMP were shown to exist predominantly in the anti-state (90%). In the case of 3'-nucleotides the enhancement of the relativity weight of sin-populations to 0.2 and 0.85 for 3'-CMP and 3'-AMP, respectively, was found. In the solution Ado both anti- and sin-state were equiprobable. Experimentally measured relaxation rates of H(8) of adenine or H (6) of cytosine nucleotides were used for determination of the time average orientation of nucleic bases towards the ribose ring in the anti-state. For all compounds investigated with the exception of 3'-AMP the \"normal\" anti-conformation was confirmed. The 3'-AMP was found to be characterized by high anti-conformation. The probable causes of the conformational situation organization and correlation between the N/S and sin/anti rations are discussed.", "contents": "[Conformation of nucleotides, oligonucleotides and their analogues in aqueous solution. II. Syn-anti-equilibrium in solutions of adenosine, 5'-AMP, 3'-AMP, 5'-CMP and 3'-CMP]. A method for determining the equilibrium constant of sin-anti-states in the aqueous solution of purine and pyrimidine nucleotides and nucleosides has been proposed. This method is based on the measurement of the spin-lattice relaxation rate of H(1') atom of purine nucleotides before and after exchange of H(8) deuterium in the case of purine nucleotides or H(1') and H(5) atoms of pyrimidine nucleotides. The results obtained were interpreted by the two-state dynamic model. The method applied for investigation of the conformation situation in solutions of Ado, 5'-AMP, 3'-AMP, 5'-CMP and 3'-CMP. 5'-AMP and 5'-CMP were shown to exist predominantly in the anti-state (90%). In the case of 3'-nucleotides the enhancement of the relativity weight of sin-populations to 0.2 and 0.85 for 3'-CMP and 3'-AMP, respectively, was found. In the solution Ado both anti- and sin-state were equiprobable. Experimentally measured relaxation rates of H(8) of adenine or H (6) of cytosine nucleotides were used for determination of the time average orientation of nucleic bases towards the ribose ring in the anti-state. For all compounds investigated with the exception of 3'-AMP the \"normal\" anti-conformation was confirmed. The 3'-AMP was found to be characterized by high anti-conformation. The probable causes of the conformational situation organization and correlation between the N/S and sin/anti rations are discussed."}
{"id": "PMID:470946", "title": "[Immunological and sedimentation analysis of UV-irradiated T4 phage DNA containing 5-bromouracil].", "content": "Conversions resulting from UV-irradiation of 5-bromouracil DNA of T4 phage were studied. The use of immunological and sedimentation techniques revealed that UV dose ranging from 1.4.10(2) to 1.4.10(3) erg/mm2 induced unwinding and single-stranded breaks in 5-bromouracil DNA which are not accompanied by debromination. Beginning with 4.10(3) erg/mm2 debromination but not degradation of DNA is detected. Doses higher than 4.10(3) erg/mm2 lead to extensive degradation of 5-bromouracil T4 DNA.", "contents": "[Immunological and sedimentation analysis of UV-irradiated T4 phage DNA containing 5-bromouracil]. Conversions resulting from UV-irradiation of 5-bromouracil DNA of T4 phage were studied. The use of immunological and sedimentation techniques revealed that UV dose ranging from 1.4.10(2) to 1.4.10(3) erg/mm2 induced unwinding and single-stranded breaks in 5-bromouracil DNA which are not accompanied by debromination. Beginning with 4.10(3) erg/mm2 debromination but not degradation of DNA is detected. Doses higher than 4.10(3) erg/mm2 lead to extensive degradation of 5-bromouracil T4 DNA."}
{"id": "PMID:470945", "title": "[Reaction of apo-aspartate aminotransferase with 5-thiophosphate analogues of the coenzyme. Split of the thiophosphate bond].", "content": "By the interaction of pyridoxamine- and pyridoxale-5'-thiophosphate with aspartate-aminotransferase complexes similar in their properties to corresponding forms of the native enzyme were obtained. Reversible convertions of the obtained complexes were performed by short time incubation with substrates. It was found that the thioester bond can be splitted as a result of incubation of the pyridoxamine-5'-thiophosphate form of the enzyme with the substrate mixture for several hours at pH 5.0. The same split took place during incubations of the complex of apo-enzyme with L-Nalpha-(pyridoxyl-5'-thiophosphate)-glutamic acid at pH 3.5 within several minutes. The split of the thioester bond was accomplished by formation of phosphate-enzyme bonds, the latter was found to be stable towards gel-filtration and denaturation, but unstable to proteolysis. The labilisation of the thiophosphate bond was explained in terms of changes of structure and specificity of the anchoring site of 5'-phosphoryl group according to the reaction coordinate.", "contents": "[Reaction of apo-aspartate aminotransferase with 5-thiophosphate analogues of the coenzyme. Split of the thiophosphate bond]. By the interaction of pyridoxamine- and pyridoxale-5'-thiophosphate with aspartate-aminotransferase complexes similar in their properties to corresponding forms of the native enzyme were obtained. Reversible convertions of the obtained complexes were performed by short time incubation with substrates. It was found that the thioester bond can be splitted as a result of incubation of the pyridoxamine-5'-thiophosphate form of the enzyme with the substrate mixture for several hours at pH 5.0. The same split took place during incubations of the complex of apo-enzyme with L-Nalpha-(pyridoxyl-5'-thiophosphate)-glutamic acid at pH 3.5 within several minutes. The split of the thioester bond was accomplished by formation of phosphate-enzyme bonds, the latter was found to be stable towards gel-filtration and denaturation, but unstable to proteolysis. The labilisation of the thiophosphate bond was explained in terms of changes of structure and specificity of the anchoring site of 5'-phosphoryl group according to the reaction coordinate."}
{"id": "PMID:470942", "title": "[Analysis of kinetics of the combined inhibition of cholinesterases].", "content": "In the paper the analysis of the possible kinetic schemes is performed for the process of the combined inhibition of cholinesterases. It is shown that the considered schemes result in the identical experimental dependencies of the inhibition constants from the concentration of the inhibitor. Based on the analysis of about 200 experiments a linear dependence was found between logarithms of constants describing the reversible and irreversible action of the inhibitor.", "contents": "[Analysis of kinetics of the combined inhibition of cholinesterases]. In the paper the analysis of the possible kinetic schemes is performed for the process of the combined inhibition of cholinesterases. It is shown that the considered schemes result in the identical experimental dependencies of the inhibition constants from the concentration of the inhibitor. Based on the analysis of about 200 experiments a linear dependence was found between logarithms of constants describing the reversible and irreversible action of the inhibitor."}
{"id": "PMID:470947", "title": "[Comparative investigation of genomes of some higher plants].", "content": "By the equilibrium ultracentrifugation in the CsCl density gradient, high-resolution thermal denaturation and kinetics of reassociation the genomes of some higher plants were investigated. It is shown that nuclear DNAs of nonsatellite species Phaseolus aureus and Brassica oleracea contain fractions, resembling DNA satellite components of Phaseolus vulgaris and Brassica nigra. These fractions are not detected as discrete components because of insufficient length of blocks, containing the sequences of satellite DNAs.", "contents": "[Comparative investigation of genomes of some higher plants]. By the equilibrium ultracentrifugation in the CsCl density gradient, high-resolution thermal denaturation and kinetics of reassociation the genomes of some higher plants were investigated. It is shown that nuclear DNAs of nonsatellite species Phaseolus aureus and Brassica oleracea contain fractions, resembling DNA satellite components of Phaseolus vulgaris and Brassica nigra. These fractions are not detected as discrete components because of insufficient length of blocks, containing the sequences of satellite DNAs."}
{"id": "PMID:470948", "title": "[Semiempirical calculations of the structure of apamine].", "content": "Conformational properties of the neurotoxin apamine were investigated starting from the known amino acid sequence. Nonvalent and electrostatic interactions, hydrogen bonding and torsional energies were computed by variation of all backbone and side chain dihedral angles. In a search for the spatial structure of the molecule, di-, tri-, tetra- etc. fragments were subsequently analyzed. As the result, the apriori calculations of the 15-residue apamine fragment revealed strong energy differentiations of forms. The lowest energy conformation has a folded backbone, with an orientation of Cys-1--Cys-11 and Cys-3--Cys-15 side chain pairs favoring the formation of disulfide bridges.", "contents": "[Semiempirical calculations of the structure of apamine]. Conformational properties of the neurotoxin apamine were investigated starting from the known amino acid sequence. Nonvalent and electrostatic interactions, hydrogen bonding and torsional energies were computed by variation of all backbone and side chain dihedral angles. In a search for the spatial structure of the molecule, di-, tri-, tetra- etc. fragments were subsequently analyzed. As the result, the apriori calculations of the 15-residue apamine fragment revealed strong energy differentiations of forms. The lowest energy conformation has a folded backbone, with an orientation of Cys-1--Cys-11 and Cys-3--Cys-15 side chain pairs favoring the formation of disulfide bridges."}
{"id": "PMID:470949", "title": "[Effect of dehydration on functioning of photosystems of higher plants].", "content": "The functional activity of both photosystems of higher plants and their thermoresistance in conditions of dehydratation of chloroplasts or subchloroplast fragments were studied. It is shown that dehydratation of the sample does not change the P700 amount capable to photooxidation. At 20 degrees in the time course of dark reduction of photooxidized P700 P(700+) in films two phases differing in rate were found. The relative contribution of each phase depends on the illumination duration. Since dehydratation blocks electron transfer between photosystems, the double phase dark reduction of P700+ in films reflects the electron flow from various components of potosystem 1 acceptor part. Dehydratation has little effect on properties of photosystem 1 acceptor part, because at low temperature the time courses of P700+ dark reduction in films and chloroplast or subchloroplast suspensions are similar. In contrast with potosystem 1, the functioning of photosystem 2, studied by light induced increase of fluorescence yield of chloroplasts, is blocked abruptly by water removal, but it could be partly restored by rehydratation of dried chloroplasts. The water removal increases the thermostability of both photosystems, however in suspension of the studied samples and also in their films photosystem 1 is more thermostable in comparison with photosystem 2.", "contents": "[Effect of dehydration on functioning of photosystems of higher plants]. The functional activity of both photosystems of higher plants and their thermoresistance in conditions of dehydratation of chloroplasts or subchloroplast fragments were studied. It is shown that dehydratation of the sample does not change the P700 amount capable to photooxidation. At 20 degrees in the time course of dark reduction of photooxidized P700 P(700+) in films two phases differing in rate were found. The relative contribution of each phase depends on the illumination duration. Since dehydratation blocks electron transfer between photosystems, the double phase dark reduction of P700+ in films reflects the electron flow from various components of potosystem 1 acceptor part. Dehydratation has little effect on properties of photosystem 1 acceptor part, because at low temperature the time courses of P700+ dark reduction in films and chloroplast or subchloroplast suspensions are similar. In contrast with potosystem 1, the functioning of photosystem 2, studied by light induced increase of fluorescence yield of chloroplasts, is blocked abruptly by water removal, but it could be partly restored by rehydratation of dried chloroplasts. The water removal increases the thermostability of both photosystems, however in suspension of the studied samples and also in their films photosystem 1 is more thermostable in comparison with photosystem 2."}
{"id": "PMID:470958", "title": "[Energy and nutrient supply during childhood. V. Iron intake (author's transl)].", "content": "The iron intake of 2--14 year old children living at home is reported. The iron intake of 2--3 year old boys amounts to 7.5 +/- 1.2 mg/day, of girls to 7.1 +/- 1.3 mg/day. The iron intake increases to 13.0 +/- 2.4 mg/day in 12--14 year old boys and to 11.6 +/- 1.6 mg/day in girls. The iron content of the food is 4.7--5.6 mg/1,000 kcal, independent of age and sex. 1/3 of the iron comes from animal foodstuffs, 2/3 from vegetable foodstuffs. After an observation period of at least 4 weeks 50% of the observed children reached their individual mean iron intake.", "contents": "[Energy and nutrient supply during childhood. V. Iron intake (author's transl)]. The iron intake of 2--14 year old children living at home is reported. The iron intake of 2--3 year old boys amounts to 7.5 +/- 1.2 mg/day, of girls to 7.1 +/- 1.3 mg/day. The iron intake increases to 13.0 +/- 2.4 mg/day in 12--14 year old boys and to 11.6 +/- 1.6 mg/day in girls. The iron content of the food is 4.7--5.6 mg/1,000 kcal, independent of age and sex. 1/3 of the iron comes from animal foodstuffs, 2/3 from vegetable foodstuffs. After an observation period of at least 4 weeks 50% of the observed children reached their individual mean iron intake."}
{"id": "PMID:470959", "title": "[Follow-up results of neonatal renal vein thrombosis after heparin treatment (author's transl)].", "content": "The authors report on their experiences gained during follow-up studies in 3 children, who as neonates had had renal venous thrombosis, and had been treated with heparin. On the basis of the relevant literature and their own observations, the authors suggest heparinisation during the acute stage.", "contents": "[Follow-up results of neonatal renal vein thrombosis after heparin treatment (author's transl)]. The authors report on their experiences gained during follow-up studies in 3 children, who as neonates had had renal venous thrombosis, and had been treated with heparin. On the basis of the relevant literature and their own observations, the authors suggest heparinisation during the acute stage."}
{"id": "PMID:470960", "title": "[Reference values of lipid metabolism in childhood (author's transl)].", "content": "Sera of 949 children were analyzed for cholesterol and triglycerides by enzymatic methods and for lipoprotein pattern by electrophoresis. For the first time all ages were examined. Two independent collectives were compared: healthy pupils and sick children without disturbances of lipid metabolism. The values of these groups showed no significant difference. In infancy cholesterol and triglycerides levels heavily depend on age, while later in childhood values change little. Highest reference values are 6.36 mmol/l (= 246 mg/dl) for cholesterol and 1.74 mmol/l (= 154 mg/dl) for triglycerides.", "contents": "[Reference values of lipid metabolism in childhood (author's transl)]. Sera of 949 children were analyzed for cholesterol and triglycerides by enzymatic methods and for lipoprotein pattern by electrophoresis. For the first time all ages were examined. Two independent collectives were compared: healthy pupils and sick children without disturbances of lipid metabolism. The values of these groups showed no significant difference. In infancy cholesterol and triglycerides levels heavily depend on age, while later in childhood values change little. Highest reference values are 6.36 mmol/l (= 246 mg/dl) for cholesterol and 1.74 mmol/l (= 154 mg/dl) for triglycerides."}
{"id": "PMID:470961", "title": "[Calcaneus osteomyelitis of newborns caused by diagnostical heel punction (author's transl)].", "content": "4 Cases of calcaneus Osteomyelitis caused by \"diagnostical heel punction\" are demonstrated. More attention should be paid to this complication which is not enough mentioned in literature.", "contents": "[Calcaneus osteomyelitis of newborns caused by diagnostical heel punction (author's transl)]. 4 Cases of calcaneus Osteomyelitis caused by \"diagnostical heel punction\" are demonstrated. More attention should be paid to this complication which is not enough mentioned in literature."}
{"id": "PMID:470962", "title": "[Aneurysmal bone cyst (author's transl)].", "content": "Aneurysmal bone cyst in two patients are described and some radiological and clinical problems discussed, especially some difficulties in establishing the X-ray diagnosis of the infrequent disease.", "contents": "[Aneurysmal bone cyst (author's transl)]. Aneurysmal bone cyst in two patients are described and some radiological and clinical problems discussed, especially some difficulties in establishing the X-ray diagnosis of the infrequent disease."}
{"id": "PMID:470965", "title": "A first exploration of a Robertsonian translocation heterozygote in the mouse for its usefulness in cytological evaluation of radiation-induced meiotic autosomal non-disjunction.", "content": "In this report some data concerning the male meiotic system of mice heterozygous for Rb(11.13)4Bnr are presented and compared with those of a chromosomally normal Swiss random-bred stock. Change of the genetic background from a C3H/Swiss hybrid situation to the fourth backcross generation (to the Swiss random-bred stock), did not alter the average frequency of aneuploid secondary spermatocytes. This was confirmed by studies on post-implantation loss. Spermatogenic characteristics of Rb4/+ mice, such as testis weight, sperm production and the number of diplotene-metaphase-I figures found in stage XII of the seminiferous epithelium, suggest delay and cell death during this period. These data support our working hypothesis that such an aberrant chromosome system may be more prone to radiation effects and therefore is promising in our cytological studies into the causes of spontaneous and in our cytological studies into the causes of spontaneous and induced autosomal non-disjunction during meiosis in the mouse.", "contents": "A first exploration of a Robertsonian translocation heterozygote in the mouse for its usefulness in cytological evaluation of radiation-induced meiotic autosomal non-disjunction. In this report some data concerning the male meiotic system of mice heterozygous for Rb(11.13)4Bnr are presented and compared with those of a chromosomally normal Swiss random-bred stock. Change of the genetic background from a C3H/Swiss hybrid situation to the fourth backcross generation (to the Swiss random-bred stock), did not alter the average frequency of aneuploid secondary spermatocytes. This was confirmed by studies on post-implantation loss. Spermatogenic characteristics of Rb4/+ mice, such as testis weight, sperm production and the number of diplotene-metaphase-I figures found in stage XII of the seminiferous epithelium, suggest delay and cell death during this period. These data support our working hypothesis that such an aberrant chromosome system may be more prone to radiation effects and therefore is promising in our cytological studies into the causes of spontaneous and in our cytological studies into the causes of spontaneous and induced autosomal non-disjunction during meiosis in the mouse."}
{"id": "PMID:470967", "title": "Characterization of DNA damages by filtration through nitrocellulose filters: a simple probe for DNA-modifying agents.", "content": "A simple technique for the detection of DNA-modifying agents is described. The double-stranded covalently closed circular DNA of phage PM2 is exposed to the modifying agent and then analysed for DNA damages by assays involving only incubation steps and filtration through nitrocellulose filters. The technique described allows the measurement of DNA modifications which lead to local denaturation of the DNA double helix, interstrand cross-links, single- and double-strand breaks, damages which render the phosphodiester bonds of the DNA sensitive to hydrolysis and damages which labilise the glycosylic bond between base and sugar moiety.", "contents": "Characterization of DNA damages by filtration through nitrocellulose filters: a simple probe for DNA-modifying agents. A simple technique for the detection of DNA-modifying agents is described. The double-stranded covalently closed circular DNA of phage PM2 is exposed to the modifying agent and then analysed for DNA damages by assays involving only incubation steps and filtration through nitrocellulose filters. The technique described allows the measurement of DNA modifications which lead to local denaturation of the DNA double helix, interstrand cross-links, single- and double-strand breaks, damages which render the phosphodiester bonds of the DNA sensitive to hydrolysis and damages which labilise the glycosylic bond between base and sugar moiety."}
{"id": "PMID:470968", "title": "Induction of sister chromatid exchanges in the central mudminnow following in vivo exposure to mutagenic agents.", "content": "Sister-chromatid exchange (SCE) studies using the central mudminnow, Umbra limi, demonstrated its usefulness as a model in vivo system for the detection of genetically active substances (mutagens and/or carcinogens). 5 days following the injection of 500 micrograms/g of 5-bromodeoxyuridine (BrdU), SCE rates were found to be quite low in the gills (2.0--3.3 SCEs/metaphase), kidneys (2.6--3.4 SCEs/metaphase), and intestines (3.7--4.5 SCEs/metaphase). However, after i.p. exposure to microgram quantities of methyl methanesulfonate (MMS) or cyclophosphamide (CP), large linear dose-dependent increases in SCE rates were observed in all tissues examined. amined. On a microgram/g basis, CP was found to be a 2--4 times more potent inducer of SCE than was MMS. Studies involving the addition of neutral red dye (NR) to the fish's aquarium water revealed that the mudminnow could concentrate the dye in its gill and kidney tissues. This dye was found to cause significant increases in SCE rates at water levels of less than 0.1 ppm. However, the concentration of dye in the tissues did not show a correlation with the SCE rate. Possible explanations for this observation are presented in the text. These findings demonstrate the feasibility of using a fish such as the mudminnow to investigate cytogenetic consequences of aquatic pollution.", "contents": "Induction of sister chromatid exchanges in the central mudminnow following in vivo exposure to mutagenic agents. Sister-chromatid exchange (SCE) studies using the central mudminnow, Umbra limi, demonstrated its usefulness as a model in vivo system for the detection of genetically active substances (mutagens and/or carcinogens). 5 days following the injection of 500 micrograms/g of 5-bromodeoxyuridine (BrdU), SCE rates were found to be quite low in the gills (2.0--3.3 SCEs/metaphase), kidneys (2.6--3.4 SCEs/metaphase), and intestines (3.7--4.5 SCEs/metaphase). However, after i.p. exposure to microgram quantities of methyl methanesulfonate (MMS) or cyclophosphamide (CP), large linear dose-dependent increases in SCE rates were observed in all tissues examined. amined. On a microgram/g basis, CP was found to be a 2--4 times more potent inducer of SCE than was MMS. Studies involving the addition of neutral red dye (NR) to the fish's aquarium water revealed that the mudminnow could concentrate the dye in its gill and kidney tissues. This dye was found to cause significant increases in SCE rates at water levels of less than 0.1 ppm. However, the concentration of dye in the tissues did not show a correlation with the SCE rate. Possible explanations for this observation are presented in the text. These findings demonstrate the feasibility of using a fish such as the mudminnow to investigate cytogenetic consequences of aquatic pollution."}
{"id": "PMID:470971", "title": "Induction of sister-chromatid exchanges in cultured human cells by an organophosphorous insecticide: malathion.", "content": "Because malathion is a widely used organophosphorous insecticide, the effects of non-toxic concentrations (2.5--40 micrograms/ml) on sister-chromatid exchange (SCE) frequencies were determined. Human fetal fibroblasts were exposed once or twice to malathion, with 20 h between exposures. A single exposure to a concentration of 40 micrograms/ml resulted in a highly significant increase in the number of SCEs. After a double exposure, a concentration of 20 micrograms/ml induced an even greater increase in SCE frequencies. Comparison of Sce frequencies after single and double exposures indicated a cumulative effect; the number of exchanges at concentrations of 5 micrograms/ml or higher was significantly greater after the double exposure. An analysis of SCEs by chromosome group showed that exchanges were distributed approximately according to chromosome length.", "contents": "Induction of sister-chromatid exchanges in cultured human cells by an organophosphorous insecticide: malathion. Because malathion is a widely used organophosphorous insecticide, the effects of non-toxic concentrations (2.5--40 micrograms/ml) on sister-chromatid exchange (SCE) frequencies were determined. Human fetal fibroblasts were exposed once or twice to malathion, with 20 h between exposures. A single exposure to a concentration of 40 micrograms/ml resulted in a highly significant increase in the number of SCEs. After a double exposure, a concentration of 20 micrograms/ml induced an even greater increase in SCE frequencies. Comparison of Sce frequencies after single and double exposures indicated a cumulative effect; the number of exchanges at concentrations of 5 micrograms/ml or higher was significantly greater after the double exposure. An analysis of SCEs by chromosome group showed that exchanges were distributed approximately according to chromosome length."}
{"id": "PMID:470972", "title": "Mutagenicity of vinyl chloride, vinylidene chloride and chloroprene in V79 Chinese hamster cells.", "content": "The mutagenicity of vinyl chloride, vinylidene chloride (1,1-dichloroethylene) and chloroprene (2-chloro-1,3-butadiene) was tested in V79 Chinese hamster cells in the presence of a 15 000 x g liver supernatant from phenobarbitone-pre-treated rats and mice. Mutations in terms of 8-azaguanine and ouabain resistance were induced in a dose-related fasion by exposure to vapour of vinyl chloride in the presence of liver supernatant from phenobarbitone-pretreated rats. Vapours of vinylidene chloride and chloroprene induced a dose-related toxicity in the presence of liver supernatant from phenobarbitone-retreated rats, but these two compounds were not mutagenic in V79 Chinese hamster cells under the present assay conditions. The results are discussed with regard to the metabolic activation of the compounds and to the correlation with their carcinogenicity in man and experimental animals.", "contents": "Mutagenicity of vinyl chloride, vinylidene chloride and chloroprene in V79 Chinese hamster cells. The mutagenicity of vinyl chloride, vinylidene chloride (1,1-dichloroethylene) and chloroprene (2-chloro-1,3-butadiene) was tested in V79 Chinese hamster cells in the presence of a 15 000 x g liver supernatant from phenobarbitone-pre-treated rats and mice. Mutations in terms of 8-azaguanine and ouabain resistance were induced in a dose-related fasion by exposure to vapour of vinyl chloride in the presence of liver supernatant from phenobarbitone-pretreated rats. Vapours of vinylidene chloride and chloroprene induced a dose-related toxicity in the presence of liver supernatant from phenobarbitone-retreated rats, but these two compounds were not mutagenic in V79 Chinese hamster cells under the present assay conditions. The results are discussed with regard to the metabolic activation of the compounds and to the correlation with their carcinogenicity in man and experimental animals."}
{"id": "PMID:471000", "title": "The use of in vitro technics to study drug-induced pancytopenia.", "content": "The confirmation of drug-induced marrow aplasia is difficult since rechallenging the patient can lead to serious morbidity. We used marrow-culture technics to challenge in vitro the bone marrow of a patient with marrow aplasia after ingestion of quinidine. There was no clinical or laboratory evidence of quinidine-mediated destruction of erythrocytes, leukocytes or platelets. By contrast, use of quinidine in combination with the patient's serum substantially inhibited in vitro growth of allogeneic marrow granulocytic and erythroid series. Furthermore, use of quinidine in combination with acute-phase serum (but not acute-phase serum alone or quinidine in combination with recovery-phase serum) inhibited growth of the patient's marrow. This observation suggests that both a transient serum factor and quinidine were responsible for the marrow aplasia. These technics could be applied with minimal risk to similar patients and would permit in vitro rechallenge with the suspected drug.", "contents": "The use of in vitro technics to study drug-induced pancytopenia. The confirmation of drug-induced marrow aplasia is difficult since rechallenging the patient can lead to serious morbidity. We used marrow-culture technics to challenge in vitro the bone marrow of a patient with marrow aplasia after ingestion of quinidine. There was no clinical or laboratory evidence of quinidine-mediated destruction of erythrocytes, leukocytes or platelets. By contrast, use of quinidine in combination with the patient's serum substantially inhibited in vitro growth of allogeneic marrow granulocytic and erythroid series. Furthermore, use of quinidine in combination with acute-phase serum (but not acute-phase serum alone or quinidine in combination with recovery-phase serum) inhibited growth of the patient's marrow. This observation suggests that both a transient serum factor and quinidine were responsible for the marrow aplasia. These technics could be applied with minimal risk to similar patients and would permit in vitro rechallenge with the suspected drug."}
{"id": "PMID:471022", "title": "Change in the content of oxidation and oligomeric products of sunflower oil during hydrogenation.", "content": "Oxidation and oligomeric products of sunflower oil were determined by gel permeation chromatography after transformation into methyl esters and prefractionation by urea. During oil refining the content of oxidation monomeric products increased from 0.9% to 1.5%, the dimer content from 0.06% to 0.32%, and the trimer content from 0.02% to 0.10%. During hydrogenation the content of polar monomeric oxidation products decreased to 0.9%, the dimer content to 0.28% and that of trimers to 0.03%. Oligomers were partly adsorbed on the surface of catalyst and carrier, because fat isolated from the catalyst contained more than 3% of oligomers. The content of oligomers established in the refined sunflower oil and in the hydrogenated sunflower oil produced on an industrial scale was lower than values given as limiting with respect to hygiene safety, sensory value and stability against oxidative rancidity.", "contents": "Change in the content of oxidation and oligomeric products of sunflower oil during hydrogenation. Oxidation and oligomeric products of sunflower oil were determined by gel permeation chromatography after transformation into methyl esters and prefractionation by urea. During oil refining the content of oxidation monomeric products increased from 0.9% to 1.5%, the dimer content from 0.06% to 0.32%, and the trimer content from 0.02% to 0.10%. During hydrogenation the content of polar monomeric oxidation products decreased to 0.9%, the dimer content to 0.28% and that of trimers to 0.03%. Oligomers were partly adsorbed on the surface of catalyst and carrier, because fat isolated from the catalyst contained more than 3% of oligomers. The content of oligomers established in the refined sunflower oil and in the hydrogenated sunflower oil produced on an industrial scale was lower than values given as limiting with respect to hygiene safety, sensory value and stability against oxidative rancidity."}
{"id": "PMID:471023", "title": "[Square-wave polarographic determination of heavy metals in vegetables with special regard to cadmium].", "content": "The cadmium contents of 104 foods mostly of vegetable origin (market samples from a highly industrialized area in the GDR) were determined by means of square-wave polarography. The values obtained are in good agreement with literature data about the cadmium contents of foods, and they are not indicative of an increased contamination of the products under investigation compared to other industrialized countries.", "contents": "[Square-wave polarographic determination of heavy metals in vegetables with special regard to cadmium]. The cadmium contents of 104 foods mostly of vegetable origin (market samples from a highly industrialized area in the GDR) were determined by means of square-wave polarography. The values obtained are in good agreement with literature data about the cadmium contents of foods, and they are not indicative of an increased contamination of the products under investigation compared to other industrialized countries."}
{"id": "PMID:471024", "title": "[Determination of the quality of injuries of potato tubers due to damage and rot].", "content": "A new method is presented for determining damage to potato tuber tissue. It is based on the fact that starch can be leached with water from the damaged tissue. The starch content of the washing water informs about the extent of tissue damage. Damage assessments of the same samples yielded correlation coefficients ranging from r = 0.8 to r = 0.9 between the determinations and the assessments. The method considers both the mechanical tissue damage and the rot.", "contents": "[Determination of the quality of injuries of potato tubers due to damage and rot]. A new method is presented for determining damage to potato tuber tissue. It is based on the fact that starch can be leached with water from the damaged tissue. The starch content of the washing water informs about the extent of tissue damage. Damage assessments of the same samples yielded correlation coefficients ranging from r = 0.8 to r = 0.9 between the determinations and the assessments. The method considers both the mechanical tissue damage and the rot."}
{"id": "PMID:471025", "title": "[Dietary interview. I. Comparison between dietary history versus repeated daily recalls].", "content": "In the course of 12 months, 43 men and 53 women aged 30-40 years were subjected to 14 24-hours recalls and one dietary history concerned with their individual dietary habits. The mean values yielded by the first and the second method for energy, 4 nutrient groups and 8 food groups were statistically evaluated and compared with each other, from which the following conclusions can be drawn as to the reliability and the precision of the dietary history: 1. The dietary history permits the quantitative estimate of the energy and nutrient intake in groups of about 50 subjects with an error of less than 10%. Underestimation of the actual food intake is more likely than overestimation. 2. The same holds true for the quantitative estimate of the consumption of foods from great food groups. The method is considerably less precise in case of single food or irregularly consumed foods. The differences between the results from the two methods ranged from 0 to 25%. 3. The precision of the individual results obtained for energy and nutrient intake from the dietary history was +/- 20% with a probability of 80-90%. In 75% of the cases, the differences in the consumption of food groups as determined by the two methods were smaller than 20%. As to single foods, however, individual misestimations ranging from 30 to 50% are to be expected.", "contents": "[Dietary interview. I. Comparison between dietary history versus repeated daily recalls]. In the course of 12 months, 43 men and 53 women aged 30-40 years were subjected to 14 24-hours recalls and one dietary history concerned with their individual dietary habits. The mean values yielded by the first and the second method for energy, 4 nutrient groups and 8 food groups were statistically evaluated and compared with each other, from which the following conclusions can be drawn as to the reliability and the precision of the dietary history: 1. The dietary history permits the quantitative estimate of the energy and nutrient intake in groups of about 50 subjects with an error of less than 10%. Underestimation of the actual food intake is more likely than overestimation. 2. The same holds true for the quantitative estimate of the consumption of foods from great food groups. The method is considerably less precise in case of single food or irregularly consumed foods. The differences between the results from the two methods ranged from 0 to 25%. 3. The precision of the individual results obtained for energy and nutrient intake from the dietary history was +/- 20% with a probability of 80-90%. In 75% of the cases, the differences in the consumption of food groups as determined by the two methods were smaller than 20%. As to single foods, however, individual misestimations ranging from 30 to 50% are to be expected."}
{"id": "PMID:471026", "title": "[Method for identifying and determining polycyclic aromatic hydrocarbons in foods, soil and drinking water].", "content": "A method is described for identifying and determining polycyclic aromatic hydrocarbons in foods, soil and drinking water that, owing to the combination of extractive, chromatographic and spectrophotometric techniques, permits to detect 0,01 microgram of bezo(a)pyrene/kg in the presence of other polycyclic aromatic hydrocarbons. The preparation, pretreatment and concentration of the samples depend upon their nature. The isolation and separation of the polycyclic aromatic hydrocarbons are achieved with the aid of two consecutive thin-layer chromatographic systems, silica gel and acetylated cellulose. The subsequent identification and determination are based on the ulta-violet spectra (limit of detection, almost 0,5 microgram/ml) and, in the nanogram range, on the in situ fluorescence spectral analysis (limit of detection, 0,005 microgram of benzo(a)pyrene/spot). Fluorescence excitation and emission spectra obtained directly from the acetylated cellulose plate permit not only the reliable identification but also the determination of the polycyclic aromatic hycrocarbons; fluorescence transfer loci obtained under various conditions, the simultaneous determination. Depending upon the kinds of sample material, the recovery values range from 75 to 99.6% with a variation coefficient between 10.3 and 22.1%. Furthermore, a limiting value method is presented that permits to take yes-no decisions, by means of thin-layer chromatographic screening without using complicated measuring techniques, indicating whether or not the respective sample complies with the benzo(a)pyrene standard.", "contents": "[Method for identifying and determining polycyclic aromatic hydrocarbons in foods, soil and drinking water]. A method is described for identifying and determining polycyclic aromatic hydrocarbons in foods, soil and drinking water that, owing to the combination of extractive, chromatographic and spectrophotometric techniques, permits to detect 0,01 microgram of bezo(a)pyrene/kg in the presence of other polycyclic aromatic hydrocarbons. The preparation, pretreatment and concentration of the samples depend upon their nature. The isolation and separation of the polycyclic aromatic hydrocarbons are achieved with the aid of two consecutive thin-layer chromatographic systems, silica gel and acetylated cellulose. The subsequent identification and determination are based on the ulta-violet spectra (limit of detection, almost 0,5 microgram/ml) and, in the nanogram range, on the in situ fluorescence spectral analysis (limit of detection, 0,005 microgram of benzo(a)pyrene/spot). Fluorescence excitation and emission spectra obtained directly from the acetylated cellulose plate permit not only the reliable identification but also the determination of the polycyclic aromatic hycrocarbons; fluorescence transfer loci obtained under various conditions, the simultaneous determination. Depending upon the kinds of sample material, the recovery values range from 75 to 99.6% with a variation coefficient between 10.3 and 22.1%. Furthermore, a limiting value method is presented that permits to take yes-no decisions, by means of thin-layer chromatographic screening without using complicated measuring techniques, indicating whether or not the respective sample complies with the benzo(a)pyrene standard."}
{"id": "PMID:471027", "title": "Bases and consequences of the newly recommended daily intakes of vitamins for the population of the German Democratic Republic.", "content": "In 1977 the official recommendations of the GDR for the daily intakes of essential nutrients were revised and extended. The criteria used to establish the revision and the extension in regard to the recommended vitamin intakes are given. For assessing the adequacy of food supplies and intakes vitamin intake data from national food consumption surveys and the weighted average recommended intakes for the entire population were compared. To give a true representation of vitamin amounts actually consumed, different forms of calculating losses on the way from harvesting or producing foods to the gullet have been applied. With the exception of tocopherol the daily intake of all vitamins considered permanently increased within the 10 years' period regarded, and the average levels of vitamin intake may appear satisfactory. Nevertheless, it is not valid to conclude that the vitamin needs of all individuals have been met.", "contents": "Bases and consequences of the newly recommended daily intakes of vitamins for the population of the German Democratic Republic. In 1977 the official recommendations of the GDR for the daily intakes of essential nutrients were revised and extended. The criteria used to establish the revision and the extension in regard to the recommended vitamin intakes are given. For assessing the adequacy of food supplies and intakes vitamin intake data from national food consumption surveys and the weighted average recommended intakes for the entire population were compared. To give a true representation of vitamin amounts actually consumed, different forms of calculating losses on the way from harvesting or producing foods to the gullet have been applied. With the exception of tocopherol the daily intake of all vitamins considered permanently increased within the 10 years' period regarded, and the average levels of vitamin intake may appear satisfactory. Nevertheless, it is not valid to conclude that the vitamin needs of all individuals have been met."}
{"id": "PMID:471028", "title": "The post-harvest fruit rots of tomato (Lycopersicum esculentum) in Nigeria.", "content": "A survey of the post-harvest fruit rot diseases of tomato was conducted in five states of Nigeria. During severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. Two types of rots, soft and dry were recognised. The soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. Erwinia carotovora, Rhizopus oryzae, R. stolonifer, Fusarium equiseti, F. nivale and F. oxysporum were established as the soft rot pathogens; while Aspergillus aculeatus, A. flavus, Cladosporium tenuissimum, Corynespora cassiicola, Curvularia lunata, Penicillium expansum P. multicolor and Rhizoctonia solani were established as the dry rot pathogens of tomato fruits in Nigeria.", "contents": "The post-harvest fruit rots of tomato (Lycopersicum esculentum) in Nigeria. A survey of the post-harvest fruit rot diseases of tomato was conducted in five states of Nigeria. During severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. Two types of rots, soft and dry were recognised. The soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. Erwinia carotovora, Rhizopus oryzae, R. stolonifer, Fusarium equiseti, F. nivale and F. oxysporum were established as the soft rot pathogens; while Aspergillus aculeatus, A. flavus, Cladosporium tenuissimum, Corynespora cassiicola, Curvularia lunata, Penicillium expansum P. multicolor and Rhizoctonia solani were established as the dry rot pathogens of tomato fruits in Nigeria."}
{"id": "PMID:471029", "title": "[Hygienic significance of sterigmatocystin in vegetable foods. 1. Analytical detection of sterigmatocystin].", "content": "The present paper gives a survey of the chemical, physical and biological properties of the mycotoxin sterigmatocystin. It must be regarded as toxic to warm-blooded animals and as cancerogenic. It is likely to occur in foods; therefore, its analytical detection is necessary. With reference to known confirmatory reactions, a method is described for the semi-quantitative determination of sterigmatocystin in fruit and vegetables. This method permits to detect 20 microgram/kg of food by means of thin-layer chromatography, after column-chromatographic purification on silica gel. The identity is confirmed by derivatization to the semi-acetal by treatment with trifluoro-acetic anhydride. The method is suited especially for the routine control of foods.", "contents": "[Hygienic significance of sterigmatocystin in vegetable foods. 1. Analytical detection of sterigmatocystin]. The present paper gives a survey of the chemical, physical and biological properties of the mycotoxin sterigmatocystin. It must be regarded as toxic to warm-blooded animals and as cancerogenic. It is likely to occur in foods; therefore, its analytical detection is necessary. With reference to known confirmatory reactions, a method is described for the semi-quantitative determination of sterigmatocystin in fruit and vegetables. This method permits to detect 20 microgram/kg of food by means of thin-layer chromatography, after column-chromatographic purification on silica gel. The identity is confirmed by derivatization to the semi-acetal by treatment with trifluoro-acetic anhydride. The method is suited especially for the routine control of foods."}
{"id": "PMID:471030", "title": "[Hygienic significance of sterigmatocystin in vegetable foods. 2. Production of sterigmatocystin by Aspergillus versicolor].", "content": "To test the suitability of fruit as an appropriate substrate for the formation of the mycotoxin sterigmatocystin, various fruit products were inoculated with a suspension of spores of a toxicogenic strain of Aspergillus versicolor. After incubation at 22 degrees C for 14 days, the fruits and juices overgrown with mould were homogenized with ethyl acetate and subjected to thinlayer chromatography. Sterigmatocystin had been formed on most products, the largest amounts being found in gooseberry preserves (9.4 microgram/g) and in grapes (10 microgram/g). The experiments show that fruit is generally suited as a substrate for the formation of sterigmatocystin; consequently, the latter is likely to occur in the presence of Aspergillus versicolor.", "contents": "[Hygienic significance of sterigmatocystin in vegetable foods. 2. Production of sterigmatocystin by Aspergillus versicolor]. To test the suitability of fruit as an appropriate substrate for the formation of the mycotoxin sterigmatocystin, various fruit products were inoculated with a suspension of spores of a toxicogenic strain of Aspergillus versicolor. After incubation at 22 degrees C for 14 days, the fruits and juices overgrown with mould were homogenized with ethyl acetate and subjected to thinlayer chromatography. Sterigmatocystin had been formed on most products, the largest amounts being found in gooseberry preserves (9.4 microgram/g) and in grapes (10 microgram/g). The experiments show that fruit is generally suited as a substrate for the formation of sterigmatocystin; consequently, the latter is likely to occur in the presence of Aspergillus versicolor."}
{"id": "PMID:471031", "title": "[Hygienic significance of sterigmatocystin in vegetable foods. 3. Occurrence of sterigmatocystin].", "content": "A total of 142 samples of vegetable foods was examined for the occurrence of sterigmatocystin. The samples examined were fruits and vegetables which had spontaneously gone mouldy or begun to rot under natural conditions on the one hand, and organoleptically impeccable fruit juices and maize specimens on the other hand. The samples were taken at the manufacturing plant or procured on the market in the framework of operative controls. Sterigmatocystin was detected in none of the samples under investigation. From this it may be concluded that the risk of its occurrence in vegetable foods is not very great in our country. Nevertheless, due to its cancerogenic and toxic properties, sterigmatocystin should remain included in the examination for mycotoxins in the framework of food control.", "contents": "[Hygienic significance of sterigmatocystin in vegetable foods. 3. Occurrence of sterigmatocystin]. A total of 142 samples of vegetable foods was examined for the occurrence of sterigmatocystin. The samples examined were fruits and vegetables which had spontaneously gone mouldy or begun to rot under natural conditions on the one hand, and organoleptically impeccable fruit juices and maize specimens on the other hand. The samples were taken at the manufacturing plant or procured on the market in the framework of operative controls. Sterigmatocystin was detected in none of the samples under investigation. From this it may be concluded that the risk of its occurrence in vegetable foods is not very great in our country. Nevertheless, due to its cancerogenic and toxic properties, sterigmatocystin should remain included in the examination for mycotoxins in the framework of food control."}
{"id": "PMID:471032", "title": "[Hygienic significance of patulin in foods. 1. Analytical detection of patulin].", "content": "The authors describe a thin-layer chromatographic method for determining patulin in fruit and vegetable products which is suited for routine work in hygiene practice. The samples are extracted with ethyl acetate, and the extracts are purified on a Florisil column. Separation is performed by means of a one-dimensional technique, using toluene/ehtyl acetate/formic acid (5 + 4 + 1), or, in the presence of interfering contaminants, by means of a two-dimensional technique, using benzene/methanol/glacial acetic acid (90 + 5 + 5) for the first run, and toluene/ethyl acetate/formic acid (5 + 4 + 1) for the second run. Patulin is detected by spraying with a benzidine solution, after chlorination. The limits of detection are 5 microgram/l of juice and 5 microgram/kg of fruit or vegetable. Derivatization with acetic anhydride/pyridine is used for corroborating the results obtained. The significance of 5-hydroxymethylfurfural as an interfering substance in apple juices is discussed.", "contents": "[Hygienic significance of patulin in foods. 1. Analytical detection of patulin]. The authors describe a thin-layer chromatographic method for determining patulin in fruit and vegetable products which is suited for routine work in hygiene practice. The samples are extracted with ethyl acetate, and the extracts are purified on a Florisil column. Separation is performed by means of a one-dimensional technique, using toluene/ehtyl acetate/formic acid (5 + 4 + 1), or, in the presence of interfering contaminants, by means of a two-dimensional technique, using benzene/methanol/glacial acetic acid (90 + 5 + 5) for the first run, and toluene/ethyl acetate/formic acid (5 + 4 + 1) for the second run. Patulin is detected by spraying with a benzidine solution, after chlorination. The limits of detection are 5 microgram/l of juice and 5 microgram/kg of fruit or vegetable. Derivatization with acetic anhydride/pyridine is used for corroborating the results obtained. The significance of 5-hydroxymethylfurfural as an interfering substance in apple juices is discussed."}
{"id": "PMID:471033", "title": "[Hygienic significance of patulin in food. 2. Occurrence of patulin in fruit and vegetables].", "content": "The analyses of more than 200 samples of various foods of plant origin showed that patulin was contained in 36% of the fresh and canned fruits infested with mould, and in 7% of the vegetables. Besides apples, pears, plums, peaches and tomatoes contained also patulin. In organoleptically impeccable fruit juices, the contamination rates were 40% (for apple juice) and 16% (for the other juices, such as sour cherry, currant, sea buckthorn juices). The patulin content varied from 20 to 200 microgram/l, the mean value being 80 microgram/l. It ranged from 0.1 to 5 microgram/g in apples and sterile apple preserves. The authors discuss the hygienic-toxicologic significance of these findings, and suggest to include patulin in the examination of foods for mycotoxins, stipulating a permissible value.", "contents": "[Hygienic significance of patulin in food. 2. Occurrence of patulin in fruit and vegetables]. The analyses of more than 200 samples of various foods of plant origin showed that patulin was contained in 36% of the fresh and canned fruits infested with mould, and in 7% of the vegetables. Besides apples, pears, plums, peaches and tomatoes contained also patulin. In organoleptically impeccable fruit juices, the contamination rates were 40% (for apple juice) and 16% (for the other juices, such as sour cherry, currant, sea buckthorn juices). The patulin content varied from 20 to 200 microgram/l, the mean value being 80 microgram/l. It ranged from 0.1 to 5 microgram/g in apples and sterile apple preserves. The authors discuss the hygienic-toxicologic significance of these findings, and suggest to include patulin in the examination of foods for mycotoxins, stipulating a permissible value."}
{"id": "PMID:471034", "title": "[Dietary interview. 2. Comparison of the results obtained with different numbers of daily recalls].", "content": "From energy, nutrient and food intake data obtained with 14 24-hours recalls from 43 male and 53 female employees, aged from 30 to 40 years, the authors calculated and compared the single and group means for 1, 3, 5, 7, 10 and 14 days, respectively. It is tried to deduce from them the lowest number of interviews necessary for reliable statements. As the intra-individual variances were greater than the variances within the groups, the diet of such a group cannot be characterized on the basis of a single interview. Statements regarding the mean intakes of energy, nutrients and high-aggregated food groups could be made for groups of subjects on the basis of 3-5 interviews, with a tolerance of +/- 5%. The stable estimation of the consumption of single foods (with a tolerance of +/- 10%) required at least 7 interviews. 10 interviews were necessary for estimating (with a tolerance of +/- 10%) the mean intakes of energy, nutrients and great food groups of single persons. These results will hold true only when the workdays and weekends are in due proportion.", "contents": "[Dietary interview. 2. Comparison of the results obtained with different numbers of daily recalls]. From energy, nutrient and food intake data obtained with 14 24-hours recalls from 43 male and 53 female employees, aged from 30 to 40 years, the authors calculated and compared the single and group means for 1, 3, 5, 7, 10 and 14 days, respectively. It is tried to deduce from them the lowest number of interviews necessary for reliable statements. As the intra-individual variances were greater than the variances within the groups, the diet of such a group cannot be characterized on the basis of a single interview. Statements regarding the mean intakes of energy, nutrients and high-aggregated food groups could be made for groups of subjects on the basis of 3-5 interviews, with a tolerance of +/- 5%. The stable estimation of the consumption of single foods (with a tolerance of +/- 10%) required at least 7 interviews. 10 interviews were necessary for estimating (with a tolerance of +/- 10%) the mean intakes of energy, nutrients and great food groups of single persons. These results will hold true only when the workdays and weekends are in due proportion."}
{"id": "PMID:471035", "title": "[Kinetics of furfural formation as related to heating, concentration and storage of orange juices and concentrates].", "content": "According to studies on an orange concentrate diluted back with distilled water to the desired extract content, the formation of furfural follows the pattern of a consequent reaction. After the induction period, the reaction curve takes a linear course cooresponding to a reaction of a zero order. The temperature dependence of the resultant reaction constant k obeys the ARRHENIUS equation and yields the same energy of activation for all the extract contents under investigation. This is a case of specific acid catalysis, because the reaction velocity is directly proportional to the hydronium ion activity. On a semilogarithmic scale, the dependence of the k values of the extract contents of the concentrates and of the re-constituted juice can be presented for various temperatures in the form of parallels. According to the literature, the amount of the furfural produced correlates with the quality of the orange juices and concentrates. Consequently, this amount might be used as an indicator in evaluating quality deteriorations due to unsuitable manufacturing and storage on the one hand, and for the computation and optimization of the manufacturing processes and parameters on the other hand.", "contents": "[Kinetics of furfural formation as related to heating, concentration and storage of orange juices and concentrates]. According to studies on an orange concentrate diluted back with distilled water to the desired extract content, the formation of furfural follows the pattern of a consequent reaction. After the induction period, the reaction curve takes a linear course cooresponding to a reaction of a zero order. The temperature dependence of the resultant reaction constant k obeys the ARRHENIUS equation and yields the same energy of activation for all the extract contents under investigation. This is a case of specific acid catalysis, because the reaction velocity is directly proportional to the hydronium ion activity. On a semilogarithmic scale, the dependence of the k values of the extract contents of the concentrates and of the re-constituted juice can be presented for various temperatures in the form of parallels. According to the literature, the amount of the furfural produced correlates with the quality of the orange juices and concentrates. Consequently, this amount might be used as an indicator in evaluating quality deteriorations due to unsuitable manufacturing and storage on the one hand, and for the computation and optimization of the manufacturing processes and parameters on the other hand."}
{"id": "PMID:471036", "title": "[Analytical methods for nitrate and nitrite in feeds. 1. Spectrophotometric determination of nitrate with o-cresol].", "content": "The o-cresol procedure which is used for determining nitrate in feeds is tested for precision. The treatment of the samples and the course of analysis are rationalized. The method is recommended as a standard procedure for biological materials; it is suited as a reference technique in elaborating new analytical procedures.", "contents": "[Analytical methods for nitrate and nitrite in feeds. 1. Spectrophotometric determination of nitrate with o-cresol]. The o-cresol procedure which is used for determining nitrate in feeds is tested for precision. The treatment of the samples and the course of analysis are rationalized. The method is recommended as a standard procedure for biological materials; it is suited as a reference technique in elaborating new analytical procedures."}
{"id": "PMID:471037", "title": "[Determination and the hygienic-toxicologic significance of patulin in fruit and fruit products].", "content": "The combination of extracting, chromatographic and fluorescence densitometric steps permits to determine patulin in fruits and fruit products with great precision and sensitivity. The limit of detection is 10 microgram/kg; the recovery rates range from 86 to 92% with a variation coefficient lying between 5.6 and 13.6%. Interferences due to patulin-simulating substances are widely excluded. Patulin concentrations ranging from 0.3 to 42 mg/kg were found in the brown-rotten portions of apples. Juices domestically prepared from healthy fruits and fruits rid of rotten portions, respectively, contained no patulin. On the contrary, patulin concentrations ranging from 0.02 to 0.3 mg/l were observed in commercial apple juices. As to products with subsequent mould infection, patulin values up to 50 mg/l were found in apple juices, and up to 0.4 mg/kg in peach preserves. In a further 24 commercial fruit and vegetable juices, patulin was not detectable even not in cider, in cereals which had gone mouldy spontaneously, and in bread samples. The fruits and fruit products were analysed not only for patulin, but also for aflatoxin and ochratoxin; the latter two, however, were found in none of the samples examined. The possibilities of manufacturing patulin-free products are discussed.", "contents": "[Determination and the hygienic-toxicologic significance of patulin in fruit and fruit products]. The combination of extracting, chromatographic and fluorescence densitometric steps permits to determine patulin in fruits and fruit products with great precision and sensitivity. The limit of detection is 10 microgram/kg; the recovery rates range from 86 to 92% with a variation coefficient lying between 5.6 and 13.6%. Interferences due to patulin-simulating substances are widely excluded. Patulin concentrations ranging from 0.3 to 42 mg/kg were found in the brown-rotten portions of apples. Juices domestically prepared from healthy fruits and fruits rid of rotten portions, respectively, contained no patulin. On the contrary, patulin concentrations ranging from 0.02 to 0.3 mg/l were observed in commercial apple juices. As to products with subsequent mould infection, patulin values up to 50 mg/l were found in apple juices, and up to 0.4 mg/kg in peach preserves. In a further 24 commercial fruit and vegetable juices, patulin was not detectable even not in cider, in cereals which had gone mouldy spontaneously, and in bread samples. The fruits and fruit products were analysed not only for patulin, but also for aflatoxin and ochratoxin; the latter two, however, were found in none of the samples examined. The possibilities of manufacturing patulin-free products are discussed."}
{"id": "PMID:471038", "title": "[Chemical modification of proteins. 5. Modification of the 11-S-globulin from sunflower seed by reaction with dialdehyde starch].", "content": "Dialdehyde starch (DAS) reacts unspecifically with the amino acid residues of the 11 S globulin from sunflower seed. The modification of the protein causes a decrease of the content of each amino acid. Their blocking reaches maximum values at high pH levels (9,5) and high concentration of protein (5%). Especially high reactivity is shown by arginine as well as by the hydrophobic amino acids isoleucine, valine, and proline, and furthermore by histidine, lysine, asparagine (aspartic acid), and glutamine (glutamic acid). By reaction with DAS at pH 8.0 70% of the amino groups are blocked within 6 h; on the contrary, glyoxale blocks only 30% of the amino groups. Owing to the blockage of charged amino acid groups, a shift of the isoelectric point of the protein to a lower pH (4,3-4,4) takes place; this effect can be followed for 2 days. As a result of the reaction with DAS, only small amounts (10-15%) of intermolecular crosslinkage products with sedimentation coefficients of 17 S and greater than 17 S were formed. But by means of SDS-gel electrophoresis, dimers and trimers of the polypeptide chains in the protein were detected.", "contents": "[Chemical modification of proteins. 5. Modification of the 11-S-globulin from sunflower seed by reaction with dialdehyde starch]. Dialdehyde starch (DAS) reacts unspecifically with the amino acid residues of the 11 S globulin from sunflower seed. The modification of the protein causes a decrease of the content of each amino acid. Their blocking reaches maximum values at high pH levels (9,5) and high concentration of protein (5%). Especially high reactivity is shown by arginine as well as by the hydrophobic amino acids isoleucine, valine, and proline, and furthermore by histidine, lysine, asparagine (aspartic acid), and glutamine (glutamic acid). By reaction with DAS at pH 8.0 70% of the amino groups are blocked within 6 h; on the contrary, glyoxale blocks only 30% of the amino groups. Owing to the blockage of charged amino acid groups, a shift of the isoelectric point of the protein to a lower pH (4,3-4,4) takes place; this effect can be followed for 2 days. As a result of the reaction with DAS, only small amounts (10-15%) of intermolecular crosslinkage products with sedimentation coefficients of 17 S and greater than 17 S were formed. But by means of SDS-gel electrophoresis, dimers and trimers of the polypeptide chains in the protein were detected."}
{"id": "PMID:471052", "title": "In vitro formation of a complex between cytoskeletal proteins of the human erythrocyte.", "content": "The formation of a high-molecular weight complex between spectrin and F-actin depends on the presence of a third cytoskeletal constituent, protein 4.1. Electron microscopy shows that in this ternary complex the actin filaments are linked by bridges, which have the appearance of spectrin. The spectrin must be in the tetrameric state for such bridges to form: the dimer is evidently univalent, for it binds but forms no cross-links. G-actin also fails to form extended complexes. It is inferred that in the native cytoskeleton the spectrin is tetrameric and associated with 4.1 and probably oligomers of actin.", "contents": "In vitro formation of a complex between cytoskeletal proteins of the human erythrocyte. The formation of a high-molecular weight complex between spectrin and F-actin depends on the presence of a third cytoskeletal constituent, protein 4.1. Electron microscopy shows that in this ternary complex the actin filaments are linked by bridges, which have the appearance of spectrin. The spectrin must be in the tetrameric state for such bridges to form: the dimer is evidently univalent, for it binds but forms no cross-links. G-actin also fails to form extended complexes. It is inferred that in the native cytoskeleton the spectrin is tetrameric and associated with 4.1 and probably oligomers of actin."}
{"id": "PMID:471053", "title": "Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen.", "content": "DNA extracted from hepatitis B virus Dane particles has been cloned in bacteria using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced from the DNA indicates that the surface antigens is a protein consisting of 226 amino acids and with a molecular weight of 25,398. The portion of the gene coding for this protein apparently contains no intervening sequences.", "contents": "Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. DNA extracted from hepatitis B virus Dane particles has been cloned in bacteria using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced from the DNA indicates that the surface antigens is a protein consisting of 226 amino acids and with a molecular weight of 25,398. The portion of the gene coding for this protein apparently contains no intervening sequences."}
{"id": "PMID:471058", "title": "Chicken ovalbumin contains an internal signal sequence.", "content": "Ovalbumin is shown to contain an internal rather than an amino-terminal signal sequence. This internal signal can be recovered in a tryptic fragment which comprises residues 229--276 of mature ovalbumin and contains a region of striking sequence homology to amino-terminal cleaved signals of two other oviduct secretory proteins. The isolated tryptic fragment is used as a probe to demonstrate that a signal receptor is present in membrane vesicles derived from the rough but not in those derived from the smooth endoplasmic reticulum.", "contents": "Chicken ovalbumin contains an internal signal sequence. Ovalbumin is shown to contain an internal rather than an amino-terminal signal sequence. This internal signal can be recovered in a tryptic fragment which comprises residues 229--276 of mature ovalbumin and contains a region of striking sequence homology to amino-terminal cleaved signals of two other oviduct secretory proteins. The isolated tryptic fragment is used as a probe to demonstrate that a signal receptor is present in membrane vesicles derived from the rough but not in those derived from the smooth endoplasmic reticulum."}
{"id": "PMID:471059", "title": "'Square arrays' in the sarcolemma of human skeletal muscle fibres.", "content": "Fast twitch fibres of rat and rabbit show rectangular patterns of intramembrane particles in freeze-fracture preparations of the sarcolemma. These 'square arrays' are almost totally absent in the slow twitch coleus muscle of rat. I report here differences in the incidence of square arrays in human fetal and adult muscle and in different fibres within a human muscle. Square arrays probably classify fast and slow twitch fibres in freeze-fracture preparations of mixed muscles.", "contents": "'Square arrays' in the sarcolemma of human skeletal muscle fibres. Fast twitch fibres of rat and rabbit show rectangular patterns of intramembrane particles in freeze-fracture preparations of the sarcolemma. These 'square arrays' are almost totally absent in the slow twitch coleus muscle of rat. I report here differences in the incidence of square arrays in human fetal and adult muscle and in different fibres within a human muscle. Square arrays probably classify fast and slow twitch fibres in freeze-fracture preparations of mixed muscles."}
{"id": "PMID:471060", "title": "Use of chlorotetracycline fluorescence to demonstrate Ca2+-induced release of Ca2+ from the sarcoplasmic reticulum of skinned cardiac cells.", "content": "It has been proposed that the trans-sarcolemmal influx of Ca2+ occurring during the plateau of the mammalian cardiac action potentials is insufficient in itself to activate the myofilaments, but can trigger a release of Ca2+ from the sarcoplasmic reticulum (SR) which is sufficient for activation. The demonstration of this Ca2+-induced release of Ca2+ relied entirely on experiments in which the tension developed by the myofilaments was used as a sensor of the changes of myoplasmic free Ca2+ concentration ([free Ca2+]) in segments of single cardiac cells from which the sarcolemma had been removed by microdissection (skinned cardiac cells). The small size of these preparations has previously prevented the use of more direct methods for the detection of myoplasmic Ca2+ movements. The present study is a direct demonstration of Ca2+-induced release of Ca2+ from the SR of skinned cardiac cells treated with chlorotetracycline (CTC), a fluorescent chelate probe which enables changes in the amount of Ca2+ bound to a variety of biological membranes or micelles to be monitored. The fluorescence increases when more Ca2+ is bound.", "contents": "Use of chlorotetracycline fluorescence to demonstrate Ca2+-induced release of Ca2+ from the sarcoplasmic reticulum of skinned cardiac cells. It has been proposed that the trans-sarcolemmal influx of Ca2+ occurring during the plateau of the mammalian cardiac action potentials is insufficient in itself to activate the myofilaments, but can trigger a release of Ca2+ from the sarcoplasmic reticulum (SR) which is sufficient for activation. The demonstration of this Ca2+-induced release of Ca2+ relied entirely on experiments in which the tension developed by the myofilaments was used as a sensor of the changes of myoplasmic free Ca2+ concentration ([free Ca2+]) in segments of single cardiac cells from which the sarcolemma had been removed by microdissection (skinned cardiac cells). The small size of these preparations has previously prevented the use of more direct methods for the detection of myoplasmic Ca2+ movements. The present study is a direct demonstration of Ca2+-induced release of Ca2+ from the SR of skinned cardiac cells treated with chlorotetracycline (CTC), a fluorescent chelate probe which enables changes in the amount of Ca2+ bound to a variety of biological membranes or micelles to be monitored. The fluorescence increases when more Ca2+ is bound."}
{"id": "PMID:471061", "title": "Specific tricyclic antidepressant binding sites in rat brain.", "content": "The discovery of high-affinity binding sites for psychoactive drugs such as benzodiazepines, opiates and neuroleptics has opened up new approaches to the study of these drugs and their mechanisms of action. Although most tricyclic antidepressants inhibit neuronal uptake of noradrenaline and serotonin, their mechanism of action remains unclear. Changes in the sensitivity of the beta-receptor after chronic tricyclic antidepressant treatment suggest that they modulate noradrenergic neurotransmission. Tricyclic antidepressants also act directly on cholinergic, histaminergic, alpha-adrenergic and serotonergic receptors. It is not clear, however, which, if any, of these effects are related to the primary antidepressant effect or whether they are simply responsible for some of the side effects. We have thus investigated the possibility that specific binding sites for tricyclic antidepressants exist in the central nervous system. So far, binding studies using 3H-labelled tricyclic antidepressant drugs have only detected binding to histaminergic H2 and cholinergic muscarinic receptors and low-affinity binding. We demonstrate here a population of specific high-affinity binding sites for 3H-imipramine on brain membranes which may be responsible for the antidepressant effects of these drugs.", "contents": "Specific tricyclic antidepressant binding sites in rat brain. The discovery of high-affinity binding sites for psychoactive drugs such as benzodiazepines, opiates and neuroleptics has opened up new approaches to the study of these drugs and their mechanisms of action. Although most tricyclic antidepressants inhibit neuronal uptake of noradrenaline and serotonin, their mechanism of action remains unclear. Changes in the sensitivity of the beta-receptor after chronic tricyclic antidepressant treatment suggest that they modulate noradrenergic neurotransmission. Tricyclic antidepressants also act directly on cholinergic, histaminergic, alpha-adrenergic and serotonergic receptors. It is not clear, however, which, if any, of these effects are related to the primary antidepressant effect or whether they are simply responsible for some of the side effects. We have thus investigated the possibility that specific binding sites for tricyclic antidepressants exist in the central nervous system. So far, binding studies using 3H-labelled tricyclic antidepressant drugs have only detected binding to histaminergic H2 and cholinergic muscarinic receptors and low-affinity binding. We demonstrate here a population of specific high-affinity binding sites for 3H-imipramine on brain membranes which may be responsible for the antidepressant effects of these drugs."}
{"id": "PMID:471063", "title": "Evidence that three structural genes code for human alkaline phosphatases.", "content": "The number of structural gene loci that code for the different molecular forms of human alkaline phosphatase is unknown. Physical properties of the enzymes, immunological data, chemical inhibition and genetic studies suggest that at least three structural genes are involved: one coding for alkaline phosphatase from placenta, another for the enzyme from intestine, and one or more for the enzymes from liver, kidney and bone. Badger and Sussman have shown that alkaline phosphatases from human liver and placenta are products of different structural genes, and Greene and Sussman have shown that alkaline phosphatase from a metastasised bronchogenic carcinoma was nearly identical to the enzyme from placenta. However, other tumour-associated alkaline phosphatases and the enzymes from normal tissue other than placenta and liver have not been identified by conclusive structural criteria, and thus it is not known whether these onco-alkaline phosphatases represent ectopic production or unusual post-translational modification of the enzymes found in normal tissues. We present here, using a sensitive peptide-mapping technique, structural evidence that the enzyme forms from liver, kidney and serum from a patient with Paget's disease of bone (osteitis deformans) are products of the same structural gene and can be easily distinguished from either the intestinal or placental isoenzymes. The technqiue seems to be useful for the classification of tumour-associated alkaline phosphatases on a structural basis.", "contents": "Evidence that three structural genes code for human alkaline phosphatases. The number of structural gene loci that code for the different molecular forms of human alkaline phosphatase is unknown. Physical properties of the enzymes, immunological data, chemical inhibition and genetic studies suggest that at least three structural genes are involved: one coding for alkaline phosphatase from placenta, another for the enzyme from intestine, and one or more for the enzymes from liver, kidney and bone. Badger and Sussman have shown that alkaline phosphatases from human liver and placenta are products of different structural genes, and Greene and Sussman have shown that alkaline phosphatase from a metastasised bronchogenic carcinoma was nearly identical to the enzyme from placenta. However, other tumour-associated alkaline phosphatases and the enzymes from normal tissue other than placenta and liver have not been identified by conclusive structural criteria, and thus it is not known whether these onco-alkaline phosphatases represent ectopic production or unusual post-translational modification of the enzymes found in normal tissues. We present here, using a sensitive peptide-mapping technique, structural evidence that the enzyme forms from liver, kidney and serum from a patient with Paget's disease of bone (osteitis deformans) are products of the same structural gene and can be easily distinguished from either the intestinal or placental isoenzymes. The technqiue seems to be useful for the classification of tumour-associated alkaline phosphatases on a structural basis."}
{"id": "PMID:471064", "title": "Interaction of C-reactive protein with artificial phosphatidylcholine bilayers.", "content": "C-Reactive protein (CRP), the most characteristic of the 'acute phase proteins' (ref. 1) is thought to participate in the mediation and/or modulation of acute inflammatory processes, but its exact function is unknown. CRP has a Ca2+-dependent binding specificity for phosphorylcholine, the polar head group of two widely distributed lipids, lecithin (phosphatidylcholine, PC) and sphingomyelin (SM). A number of observations suggest that at least some of the biological activities of CRP depend on its interaction with phospholipids of cell membranes. In addition, interaction of CRP with PC- and SM-containing lipid dispersions and with PC-containing liposomes can activate the complement system. We report here that binding of CRP to model membranes of PC requires the incorporation into the bilayer of lysophosphatidylcholine (LPC). Thus, a disturbance of the molecular organisation of the bilayer appears to be necessary for binding of CRP. These findings provide a possible biochemical explanation for binding of CRP to damaged but not intact cell membranes and might be relevant to its biological function.", "contents": "Interaction of C-reactive protein with artificial phosphatidylcholine bilayers. C-Reactive protein (CRP), the most characteristic of the 'acute phase proteins' (ref. 1) is thought to participate in the mediation and/or modulation of acute inflammatory processes, but its exact function is unknown. CRP has a Ca2+-dependent binding specificity for phosphorylcholine, the polar head group of two widely distributed lipids, lecithin (phosphatidylcholine, PC) and sphingomyelin (SM). A number of observations suggest that at least some of the biological activities of CRP depend on its interaction with phospholipids of cell membranes. In addition, interaction of CRP with PC- and SM-containing lipid dispersions and with PC-containing liposomes can activate the complement system. We report here that binding of CRP to model membranes of PC requires the incorporation into the bilayer of lysophosphatidylcholine (LPC). Thus, a disturbance of the molecular organisation of the bilayer appears to be necessary for binding of CRP. These findings provide a possible biochemical explanation for binding of CRP to damaged but not intact cell membranes and might be relevant to its biological function."}
{"id": "PMID:471065", "title": "Evidence for the bilobal nature of diferric rabbit plasma transferrin.", "content": "Plasma transferrin is involved in iron transport within the circulatory system of vertebrates, and provides an iron source for haemoglobin synthesis and other metabolic requirements. However, despite extensive studies by spectroscopic, biochemical and physiological techniques, the nature of iron binding and the mechanisms of uptake and release of iron are not fully understood. Plasma transferrins are monomeric glycoproteins with a molecular weight of approximately 80,000 (ref. 2); they have two similar and very strong binding sites for Fe(III), together with two associated anion binding sites. Fragmentation studies on various transferrins have shown that the polypeptide chain is composed of two domains formed from the N-terminal and C-terminal halves of the polypeptide chain. Each domain contains one metal binding site. The marked sequence similarities which exist between the two halves may reflect a doubling of an ancestral structural gene during the phylogenetic development of the protein. Preliminary crystallographic investigations of diferric rabbit plasma transferrin have been reported from this laboratory. We now report initial studies of the X-ray structure determination of dife-ric rabbit plasma transferrin which have led to a 6-A resolution electron density map.", "contents": "Evidence for the bilobal nature of diferric rabbit plasma transferrin. Plasma transferrin is involved in iron transport within the circulatory system of vertebrates, and provides an iron source for haemoglobin synthesis and other metabolic requirements. However, despite extensive studies by spectroscopic, biochemical and physiological techniques, the nature of iron binding and the mechanisms of uptake and release of iron are not fully understood. Plasma transferrins are monomeric glycoproteins with a molecular weight of approximately 80,000 (ref. 2); they have two similar and very strong binding sites for Fe(III), together with two associated anion binding sites. Fragmentation studies on various transferrins have shown that the polypeptide chain is composed of two domains formed from the N-terminal and C-terminal halves of the polypeptide chain. Each domain contains one metal binding site. The marked sequence similarities which exist between the two halves may reflect a doubling of an ancestral structural gene during the phylogenetic development of the protein. Preliminary crystallographic investigations of diferric rabbit plasma transferrin have been reported from this laboratory. We now report initial studies of the X-ray structure determination of dife-ric rabbit plasma transferrin which have led to a 6-A resolution electron density map."}
{"id": "PMID:471074", "title": "Comparison of sodium nitroprusside and isoprenaline aerosols in histamine-induced bronchial asthma of the guinea pig.", "content": "1. Sodium nitroprusside (NP) is a potent relaxant of the guinea pig's tracheal smooth muscle contracted by carbachol or histamine. 2. At high doses, aerosols of NP delay the occurrence of symptoms of bronchial asthma in guinea pigs subjected to a histamine aerosol. However, the beta-adrenergic bronchodilator isoprenaline is at least 2,600 times more potent as an anti-asthmatic than NP on a molar basis. 3 NP and isoprenaline both reduce the blood pressure in rats when given either as infusion or as aerosol. The aerosol/infusion dosage ratio for comparable reductions of blood pressure were 1 for isoprenaline, and 10 for NP. After cessation of the application of the NP aerosol, the blood pressure rose more than twice as fast (t1/2 of blood pressure recovery) than after cessation of the application of the isoprenaline aerosol. 4. The poor antiasthmatic effect of NP aerosols may result from low permeability of the bronchial epithelium to the NP anion, from partial destruction in the airways or during tissue permeation, and from short half life of NP in the circulating blood. 5. Because of its inferiority to known bronchodilators, aerosols of NP cannot be recommended for the treatment of acute bronchial asthma.", "contents": "Comparison of sodium nitroprusside and isoprenaline aerosols in histamine-induced bronchial asthma of the guinea pig. 1. Sodium nitroprusside (NP) is a potent relaxant of the guinea pig's tracheal smooth muscle contracted by carbachol or histamine. 2. At high doses, aerosols of NP delay the occurrence of symptoms of bronchial asthma in guinea pigs subjected to a histamine aerosol. However, the beta-adrenergic bronchodilator isoprenaline is at least 2,600 times more potent as an anti-asthmatic than NP on a molar basis. 3 NP and isoprenaline both reduce the blood pressure in rats when given either as infusion or as aerosol. The aerosol/infusion dosage ratio for comparable reductions of blood pressure were 1 for isoprenaline, and 10 for NP. After cessation of the application of the NP aerosol, the blood pressure rose more than twice as fast (t1/2 of blood pressure recovery) than after cessation of the application of the isoprenaline aerosol. 4. The poor antiasthmatic effect of NP aerosols may result from low permeability of the bronchial epithelium to the NP anion, from partial destruction in the airways or during tissue permeation, and from short half life of NP in the circulating blood. 5. Because of its inferiority to known bronchodilators, aerosols of NP cannot be recommended for the treatment of acute bronchial asthma."}
{"id": "PMID:471075", "title": "The affinity of atropine for muscarine receptors in human sphincter pupillae.", "content": "Concentration-dependent contractions in response to carbachol were determined on isolated pieces of human iris. Atropine competivtively antagonized the effect of carbachol. An apparent dissociation equilibrium constant of 0.4--0.7 nM was estimated for the muscarine receptor-atropine complex.", "contents": "The affinity of atropine for muscarine receptors in human sphincter pupillae. Concentration-dependent contractions in response to carbachol were determined on isolated pieces of human iris. Atropine competivtively antagonized the effect of carbachol. An apparent dissociation equilibrium constant of 0.4--0.7 nM was estimated for the muscarine receptor-atropine complex."}
{"id": "PMID:471079", "title": "Baclofen and muscimol: behavioural and neurochemical sequelae of unilateral intranigral administration and effects on 3H-GABA receptor binding.", "content": "Log dose-response curves for induction of contralateral rotational behaviour in the rat by unilateral intranigral injections of the GABA agonist muscimol and the GABA analogue baclofen have been compared. Baclofen, 5--1000 ng, produced a maximal rotational response that was only 40% of that produced by 0.25--100 ng muscimol, and log dose-response curves failed to show parallelism. The behavioural effects of both drugs were only weakly antagonised by haloperidol and were not antagonised by 6-hydroxydopamine lesions of ipsilateral dopamine (DA) neurons, indicating that these responses were independent of DAergic mechanisms. The effects of baclofen were weakly antagonised by picrotoxin. Intranigral muscimol and baclofen substantially elevated striatal DA concentrations. While muscimol also substantially elevated striatal dihydroxyphenylacetic acid (DOPAC) but not homovanillic acid (HVA), bactofen did not significantly effect either DOPAC or HVA. Baclofein, GABA and muscimol displaced specific 3H-GABA binding in vitro with IC50's of 40 micron, 400 nM and 40 nM respectively. These results indicate that muscimol and baclofen do not act via a unitary GABAergic mechanism, but suggest that baclofen may be a partial GABA agonist, at least at nigral GABA receptors.", "contents": "Baclofen and muscimol: behavioural and neurochemical sequelae of unilateral intranigral administration and effects on 3H-GABA receptor binding. Log dose-response curves for induction of contralateral rotational behaviour in the rat by unilateral intranigral injections of the GABA agonist muscimol and the GABA analogue baclofen have been compared. Baclofen, 5--1000 ng, produced a maximal rotational response that was only 40% of that produced by 0.25--100 ng muscimol, and log dose-response curves failed to show parallelism. The behavioural effects of both drugs were only weakly antagonised by haloperidol and were not antagonised by 6-hydroxydopamine lesions of ipsilateral dopamine (DA) neurons, indicating that these responses were independent of DAergic mechanisms. The effects of baclofen were weakly antagonised by picrotoxin. Intranigral muscimol and baclofen substantially elevated striatal DA concentrations. While muscimol also substantially elevated striatal dihydroxyphenylacetic acid (DOPAC) but not homovanillic acid (HVA), bactofen did not significantly effect either DOPAC or HVA. Baclofein, GABA and muscimol displaced specific 3H-GABA binding in vitro with IC50's of 40 micron, 400 nM and 40 nM respectively. These results indicate that muscimol and baclofen do not act via a unitary GABAergic mechanism, but suggest that baclofen may be a partial GABA agonist, at least at nigral GABA receptors."}
{"id": "PMID:471080", "title": "Micropuncture studies on the renal handling of 2,4-diamino-6,7-dimethylpteridine.", "content": "The excretion of the diuretic substance DADMP (I,4-diamino-6.7-dimethylpteridine) and of DMP (6.7-dimethylpterin) was studied on single nephrons of the rat kidney using microperfusion and microinjection techniques. In the proximal tubule only DADMP was reabsorbed to a significant degree. Fractional reabsorption rate was independent of the load applied and the permeability constant was found to be 2.2 . 10(-4) cm . s-1. Similar results were obtained in nephrons in which the substances, with inulin, were injected from middle proximal tubular puncture sites and recovered in the urine. DMP appeared in the urine quantitatively and simultaneously with the injected inulin. DADMP recovery, however, was only 20--30% of the injected load during the injection period and after 2 h some 70% was recovered from the urine of both kidneys. The reabsorbed fractions were independent of the loads applied, which varied between 2 . 10(-13) mol . min-1 and 10(-9) mol . min-1. A comparison of the microperfusion and the microinfusion data suggests that the reabsorption of DADMP occurs predominantly in the proximal convolution, and it appears that the differences between the renal handling of DMP and DADMP are explicable by their different lipid solubilities.", "contents": "Micropuncture studies on the renal handling of 2,4-diamino-6,7-dimethylpteridine. The excretion of the diuretic substance DADMP (I,4-diamino-6.7-dimethylpteridine) and of DMP (6.7-dimethylpterin) was studied on single nephrons of the rat kidney using microperfusion and microinjection techniques. In the proximal tubule only DADMP was reabsorbed to a significant degree. Fractional reabsorption rate was independent of the load applied and the permeability constant was found to be 2.2 . 10(-4) cm . s-1. Similar results were obtained in nephrons in which the substances, with inulin, were injected from middle proximal tubular puncture sites and recovered in the urine. DMP appeared in the urine quantitatively and simultaneously with the injected inulin. DADMP recovery, however, was only 20--30% of the injected load during the injection period and after 2 h some 70% was recovered from the urine of both kidneys. The reabsorbed fractions were independent of the loads applied, which varied between 2 . 10(-13) mol . min-1 and 10(-9) mol . min-1. A comparison of the microperfusion and the microinfusion data suggests that the reabsorption of DADMP occurs predominantly in the proximal convolution, and it appears that the differences between the renal handling of DMP and DADMP are explicable by their different lipid solubilities."}
{"id": "PMID:471081", "title": "Decreased sensitivity of isolated hepatocytes from baby rats, from regenerating and from poisoned livers to phalloidin.", "content": "Isolated hepatocytes, prepared from 5 day old rats, from regenerating livers of from livers after poisoning with carbon tetrachloride, are less sensitive to phalloidin in vitro than hepatocytes from untreated adult controls. The time course of the reduced susceptibility to phalloidin was compared with the ability of hepatocytes to take up bile acids under various conditions. SDS-electrophoresis of cell lysates gave no evidence for decreased levels of actin in cells with reduced sensitivity to phalloidin. In contrast, there was a good relationship between the active uptake of bile acids and the sensitivity of hepatocytes to phalloidin. The decreased response of hepatocytes from baby rats, from regenerating livers or from poisoned livers to phalloidin is more probably related to differences in phalloidin uptake than to a reduced endowment with microfilamentous structures.", "contents": "Decreased sensitivity of isolated hepatocytes from baby rats, from regenerating and from poisoned livers to phalloidin. Isolated hepatocytes, prepared from 5 day old rats, from regenerating livers of from livers after poisoning with carbon tetrachloride, are less sensitive to phalloidin in vitro than hepatocytes from untreated adult controls. The time course of the reduced susceptibility to phalloidin was compared with the ability of hepatocytes to take up bile acids under various conditions. SDS-electrophoresis of cell lysates gave no evidence for decreased levels of actin in cells with reduced sensitivity to phalloidin. In contrast, there was a good relationship between the active uptake of bile acids and the sensitivity of hepatocytes to phalloidin. The decreased response of hepatocytes from baby rats, from regenerating livers or from poisoned livers to phalloidin is more probably related to differences in phalloidin uptake than to a reduced endowment with microfilamentous structures."}
{"id": "PMID:471082", "title": "A material with opioid activity in bovine milk and milk products.", "content": "Chloroform-methanol extracts of lyophilized milk, of commercially available dried milk or baby food and of casein digests were tested for opioid activity on the guinea-pig ileum longitudinal muscle-myenteric plexus preparation. Compounds with opioid activity--which proved to be resistant to peptidases--were detected in certain batches of baby food, casein digest, and cow milk in considerably varying amounts.", "contents": "A material with opioid activity in bovine milk and milk products. Chloroform-methanol extracts of lyophilized milk, of commercially available dried milk or baby food and of casein digests were tested for opioid activity on the guinea-pig ileum longitudinal muscle-myenteric plexus preparation. Compounds with opioid activity--which proved to be resistant to peptidases--were detected in certain batches of baby food, casein digest, and cow milk in considerably varying amounts."}
{"id": "PMID:471110", "title": "[Organization of neurons in the cat Clare-Bishop area which react to photic stimulation].", "content": "Organization of neurons in Clare-Bishop associative cortex was investigated by means of microelectrode vertical penetrations and recording of the neuronal responses to visual stimulation. Of 463 electrode penetrations 255 showed responses to visual stimulation. In 131 penetrations only 1 or 2 visually driven neurons were noted, in 55 cases more than 3 neurons were registered. Of 55 investigated penetrations 8 showed distinct columnar organization, i.e. all neurons in each column had similar characteristics. In 24 penetrations neurons were organized in groups of 2 or 3 similar neurons intermingled with neurons of other types. In 18 penetrations a great degree of overlapping of the receptive fields of neurons in a single column was observed. In 5 penetrations a chaotic organization of different neurons was found. A conclusion is made that the columnar organization of visually driven neurons as a functional cortical unit can not be regarded as a prominent feature in Clare-Bishop area.", "contents": "[Organization of neurons in the cat Clare-Bishop area which react to photic stimulation]. Organization of neurons in Clare-Bishop associative cortex was investigated by means of microelectrode vertical penetrations and recording of the neuronal responses to visual stimulation. Of 463 electrode penetrations 255 showed responses to visual stimulation. In 131 penetrations only 1 or 2 visually driven neurons were noted, in 55 cases more than 3 neurons were registered. Of 55 investigated penetrations 8 showed distinct columnar organization, i.e. all neurons in each column had similar characteristics. In 24 penetrations neurons were organized in groups of 2 or 3 similar neurons intermingled with neurons of other types. In 18 penetrations a great degree of overlapping of the receptive fields of neurons in a single column was observed. In 5 penetrations a chaotic organization of different neurons was found. A conclusion is made that the columnar organization of visually driven neurons as a functional cortical unit can not be regarded as a prominent feature in Clare-Bishop area."}
{"id": "PMID:471111", "title": "[Comparative characteristics of the direct influences of the perforant pathway on neurons in hippocampal fields CA1 and CA3 in vitro].", "content": "The effect of the perforant path stimulation on the CA1 and CA3 neurons was investigated in incubated slices of the guinea pig hippocampus. Spike generation was observed in both fields during stimulation of the perforant path. The majority of the CA1 neurons followed rhythmic stimulation up to 30-80/c. The CA3 neurons responded only to low-frequency stimulation (up to 5/c). The posttetanic potentiation of responses to the perforant path stimulation was observed in both hippocampal fields.", "contents": "[Comparative characteristics of the direct influences of the perforant pathway on neurons in hippocampal fields CA1 and CA3 in vitro]. The effect of the perforant path stimulation on the CA1 and CA3 neurons was investigated in incubated slices of the guinea pig hippocampus. Spike generation was observed in both fields during stimulation of the perforant path. The majority of the CA1 neurons followed rhythmic stimulation up to 30-80/c. The CA3 neurons responded only to low-frequency stimulation (up to 5/c). The posttetanic potentiation of responses to the perforant path stimulation was observed in both hippocampal fields."}
{"id": "PMID:471112", "title": "[Myelo- and cytoarchitectonics of the cat pallidum].", "content": "600 thick serial frontal and sagittal sections of the cat cerebrum were made. Using the new controlled impregnation technique, it was shown that the pallidum is crossed by numerous transit axons bundles (TAB) consisting of thin myelinated and unmyelinated axons each 0.2-0.8 micron in diameter. TAB pass through the dorsal part of the putamen and the whole pallidum parallel to the sagittal plane and perpendicular to the frontal plane. Such TAB contain not less than 10 million axons in the pallidum of a hemisphere. The TAB are mainly related to the capsula interna and to the sensory-motor cortex. The cyto- and synaptoarchitectonics of the pallidum is described.", "contents": "[Myelo- and cytoarchitectonics of the cat pallidum]. 600 thick serial frontal and sagittal sections of the cat cerebrum were made. Using the new controlled impregnation technique, it was shown that the pallidum is crossed by numerous transit axons bundles (TAB) consisting of thin myelinated and unmyelinated axons each 0.2-0.8 micron in diameter. TAB pass through the dorsal part of the putamen and the whole pallidum parallel to the sagittal plane and perpendicular to the frontal plane. Such TAB contain not less than 10 million axons in the pallidum of a hemisphere. The TAB are mainly related to the capsula interna and to the sensory-motor cortex. The cyto- and synaptoarchitectonics of the pallidum is described."}
{"id": "PMID:471113", "title": "[Activity of interneurons of the lumbar region of the spinal cord during fictive locomotion of thalamic cats].", "content": "Impulse activity of lumbar interneurons was recorded extracellularly during fictious locomotion of thalamic cats. Neurons whose activity was modulated in the rhythm of fictitious locomotion were revealed in the lateral intermediate zone and in the lateral ventral born. 41.2% of these neurons were mainly activated at the phase of \"flexion\", 48.5%--at the phase of \"extension\" and 10.3%--at both phases. Neurons with tonically increasing or decreasing activity during rhythmic efferent discharges and neurons whose activity did not change during fictitious locomotion were also observed. All these types of neurons were similarly activated during late discharges in efferent nerves. But in this case the depth of modulation of neuronal activity was lower than during fictitious locomotion. Afferent inputs to the recorded interneurons were also investigated. The neuronal organization of the spinal locomotor generator is discussed on the basis of the obtained data.", "contents": "[Activity of interneurons of the lumbar region of the spinal cord during fictive locomotion of thalamic cats]. Impulse activity of lumbar interneurons was recorded extracellularly during fictious locomotion of thalamic cats. Neurons whose activity was modulated in the rhythm of fictitious locomotion were revealed in the lateral intermediate zone and in the lateral ventral born. 41.2% of these neurons were mainly activated at the phase of \"flexion\", 48.5%--at the phase of \"extension\" and 10.3%--at both phases. Neurons with tonically increasing or decreasing activity during rhythmic efferent discharges and neurons whose activity did not change during fictitious locomotion were also observed. All these types of neurons were similarly activated during late discharges in efferent nerves. But in this case the depth of modulation of neuronal activity was lower than during fictitious locomotion. Afferent inputs to the recorded interneurons were also investigated. The neuronal organization of the spinal locomotor generator is discussed on the basis of the obtained data."}
{"id": "PMID:471114", "title": "[Types of skeletal muscle motor unit activity during cold tremor].", "content": "The impulse activity of 184 single motor units of the sartorius muscle was investigated in anesthetized cats during cold tremor. Motor units whose impulse activity was connected with respiration cycles and motor units functioning independently of respiration were detected. The functions of both types of the motor units are characterized by the same average rate (4-12 imp/s) and low variability of interspike intervals. Additional thermal stimulation of the vascular thermoreceptors and changes in the rate and amplitude of respiratory excursions caused similar responses of the motor units of both types. It is suggested that motor units of both types have the same thresholds and belong to the phasic slow motor units.", "contents": "[Types of skeletal muscle motor unit activity during cold tremor]. The impulse activity of 184 single motor units of the sartorius muscle was investigated in anesthetized cats during cold tremor. Motor units whose impulse activity was connected with respiration cycles and motor units functioning independently of respiration were detected. The functions of both types of the motor units are characterized by the same average rate (4-12 imp/s) and low variability of interspike intervals. Additional thermal stimulation of the vascular thermoreceptors and changes in the rate and amplitude of respiratory excursions caused similar responses of the motor units of both types. It is suggested that motor units of both types have the same thresholds and belong to the phasic slow motor units."}
{"id": "PMID:471115", "title": "[Relationship between the magnitude of the inflowing current of isolated Limnea stagnalis mollusk neurons and the external concentration of the current-bearing calcium and strontium ions].", "content": "The dose-inward current relationships for current-carrying ions Ca2+ and Sr2+ were studied by means of intracellular dialysis and voltage clamp in isolated neurons of the mollusc Limnea stagnalis. For Ca2+ and Sr2+ ions these relationships had ceiling. The data obtained are explained in terms of activation theory using two-barrier model of calcium channel. Values of velocity constants vx for ion x2+ transition over the second energetic barrier and of constants Kx corresponding to effectiveness of binding in the channel's entrance were determined: vca:vsr = 1:2; KCa = 1400 M-1; KSr = 270 M-1.", "contents": "[Relationship between the magnitude of the inflowing current of isolated Limnea stagnalis mollusk neurons and the external concentration of the current-bearing calcium and strontium ions]. The dose-inward current relationships for current-carrying ions Ca2+ and Sr2+ were studied by means of intracellular dialysis and voltage clamp in isolated neurons of the mollusc Limnea stagnalis. For Ca2+ and Sr2+ ions these relationships had ceiling. The data obtained are explained in terms of activation theory using two-barrier model of calcium channel. Values of velocity constants vx for ion x2+ transition over the second energetic barrier and of constants Kx corresponding to effectiveness of binding in the channel's entrance were determined: vca:vsr = 1:2; KCa = 1400 M-1; KSr = 270 M-1."}
{"id": "PMID:471118", "title": "[Electrophysiologic analysis of interaction between afferent inputs to the rat neostriatum].", "content": "The interaction of the efferent inputs of the caudate nucleus in the region of their maximal overlapping was studied by paired stimulation. The stimulation of the n. paracentralis and cortex resulted in an increased amplitude of the tested response. On the contrary, the tested responses to stimulation of substantia nigra (zona compacta) and n. raphe (dorsal region) were inhibited. The paired stimulation showed a particular hierarchy of different afferent inputs as to their mutual influence: frontal cortex--thalamic intralaminar nucleus--midbrain structures. The afferent inputs from higher levels, while molulating the activity of those from lower ones, do not reliably change their own activity under the action of the latter. Possible mechanisms of this modulation are discussed.", "contents": "[Electrophysiologic analysis of interaction between afferent inputs to the rat neostriatum]. The interaction of the efferent inputs of the caudate nucleus in the region of their maximal overlapping was studied by paired stimulation. The stimulation of the n. paracentralis and cortex resulted in an increased amplitude of the tested response. On the contrary, the tested responses to stimulation of substantia nigra (zona compacta) and n. raphe (dorsal region) were inhibited. The paired stimulation showed a particular hierarchy of different afferent inputs as to their mutual influence: frontal cortex--thalamic intralaminar nucleus--midbrain structures. The afferent inputs from higher levels, while molulating the activity of those from lower ones, do not reliably change their own activity under the action of the latter. Possible mechanisms of this modulation are discussed."}
{"id": "PMID:471119", "title": "[Critical evaluation of the searching technic of the polarization-sensitive neurons of insects].", "content": "The demerit of the light stimulator used by the authors was the presence of lenses between the eye and the observed light source. Apart from the image of the aperture diaphragm, the images of some details and reflections were formed. The terminal lens of the stimulator and the eye optics can divide the main and secondary images of the retina. Therefore the reactions of insect neurons to polarized light described previously could be explored also as the responses to flickers in secondary images.", "contents": "[Critical evaluation of the searching technic of the polarization-sensitive neurons of insects]. The demerit of the light stimulator used by the authors was the presence of lenses between the eye and the observed light source. Apart from the image of the aperture diaphragm, the images of some details and reflections were formed. The terminal lens of the stimulator and the eye optics can divide the main and secondary images of the retina. Therefore the reactions of insect neurons to polarized light described previously could be explored also as the responses to flickers in secondary images."}
{"id": "PMID:471120", "title": "Calculations of theoretical indices of carcinogenic activity including the effect of medium.", "content": "Relation between the electronic structure of aromatic compounds and their carcinogenic activity has been correlated. Hypotheses of K, L regions have been employed. Possibilities of better theoretical description of carcinogenic activity have been examined by including the effect of medium and by using a more exact quantum--chemical method. Theoretical carcinogenic activity of some compounds so far experimentally not tested has been estimated.", "contents": "Calculations of theoretical indices of carcinogenic activity including the effect of medium. Relation between the electronic structure of aromatic compounds and their carcinogenic activity has been correlated. Hypotheses of K, L regions have been employed. Possibilities of better theoretical description of carcinogenic activity have been examined by including the effect of medium and by using a more exact quantum--chemical method. Theoretical carcinogenic activity of some compounds so far experimentally not tested has been estimated."}
{"id": "PMID:471121", "title": "Inhibitory effect of the antioxidant butylated hydroxyanisole on the activation of the carcinogen benzo(a)pyrene.", "content": "The effect of the antioxidant butylated hydroxyanisole (BHA) on benzo(a)pyrene (BP) metabolites binding to DNA, percentage of diols and phenols in the total amount of BP metabolites extracted to organic solvent, were studied in : incubation mixture of rat liver microsomes, cultured mouse embryo cells, human skin, and human lymphocytes. The amount of BP metabolites bound to DNA in mouse embryo cells and human skin decreased in the presence of BHA. No effect was found in human lymphocytes. Percent of phenols increased in mouse embryo cell cultures; percent of diols decreased in cultures of human skin and lymphocytes.", "contents": "Inhibitory effect of the antioxidant butylated hydroxyanisole on the activation of the carcinogen benzo(a)pyrene. The effect of the antioxidant butylated hydroxyanisole (BHA) on benzo(a)pyrene (BP) metabolites binding to DNA, percentage of diols and phenols in the total amount of BP metabolites extracted to organic solvent, were studied in : incubation mixture of rat liver microsomes, cultured mouse embryo cells, human skin, and human lymphocytes. The amount of BP metabolites bound to DNA in mouse embryo cells and human skin decreased in the presence of BHA. No effect was found in human lymphocytes. Percent of phenols increased in mouse embryo cell cultures; percent of diols decreased in cultures of human skin and lymphocytes."}
{"id": "PMID:471122", "title": "Differences in the purification effect of carcinoembryonic antigen (CEA) from the three different hepatic metastases of rectum carcinoma.", "content": "Carcinoembryonic actigen (CEA) was purified from three liver metastases of carcinoma recti by a conventional procedure involving perchloric acid extraction of tumor tissue and fractionation of the extract by gel filtrations on Sepharose 4B and Sephadex G-200. The preparations obtained showed a different degree of heterogeneity in SDS-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis, and also a different antigenic activity. One of preparations obtained was a homogeneous CEA by all these criteria, and two others showed heterogeneity and lower antigenic activity. The most heterogeneous CEA preparation was further purified by the affinity chromatography on Concanavalin A-Sepharose 4B and by DEAE-Sephadex A-50 chromatography and the usefulness of these procedures for purification of CEA is compared.", "contents": "Differences in the purification effect of carcinoembryonic antigen (CEA) from the three different hepatic metastases of rectum carcinoma. Carcinoembryonic actigen (CEA) was purified from three liver metastases of carcinoma recti by a conventional procedure involving perchloric acid extraction of tumor tissue and fractionation of the extract by gel filtrations on Sepharose 4B and Sephadex G-200. The preparations obtained showed a different degree of heterogeneity in SDS-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis, and also a different antigenic activity. One of preparations obtained was a homogeneous CEA by all these criteria, and two others showed heterogeneity and lower antigenic activity. The most heterogeneous CEA preparation was further purified by the affinity chromatography on Concanavalin A-Sepharose 4B and by DEAE-Sephadex A-50 chromatography and the usefulness of these procedures for purification of CEA is compared."}
{"id": "PMID:471123", "title": "Changes in hexokinase properties during transformation and subsequent proliferation of liver cells.", "content": "The activity and subcellular distribution of hexokinase in liver and several hepatomas were studied. The differences were found in the behavior of this enzyme in spontaneous, primary induced and transplantable hepatomas as compared with normal liver.", "contents": "Changes in hexokinase properties during transformation and subsequent proliferation of liver cells. The activity and subcellular distribution of hexokinase in liver and several hepatomas were studied. The differences were found in the behavior of this enzyme in spontaneous, primary induced and transplantable hepatomas as compared with normal liver."}
{"id": "PMID:471124", "title": "DNA methylation in various growth phases of cultured L5178Y murine lymphoblasts.", "content": "The extent of methylation of DNA during the transition of cells from exponential phase of growth to the saturation density has been studied in cultured L5178Y mouse lymphoma cells by the comparison of the degree of 5-methylcytosine formation and the stability of this preformed base in various periods of their growth. It has been found that while the degree of 5-methylcytosine formation gradually fell down, the level of this base preformed during the exponential growth was stable during the subsequent periods until the saturation density was reached. At that state some small and variable loss of the base was found.", "contents": "DNA methylation in various growth phases of cultured L5178Y murine lymphoblasts. The extent of methylation of DNA during the transition of cells from exponential phase of growth to the saturation density has been studied in cultured L5178Y mouse lymphoma cells by the comparison of the degree of 5-methylcytosine formation and the stability of this preformed base in various periods of their growth. It has been found that while the degree of 5-methylcytosine formation gradually fell down, the level of this base preformed during the exponential growth was stable during the subsequent periods until the saturation density was reached. At that state some small and variable loss of the base was found."}
{"id": "PMID:471127", "title": "Nucleolar morphology in estimation of urinary bladder cancer in man: study on surface contact preparations.", "content": "The special feature of nucleolar morphology which reflects the intensity of ribosomal RNA precursor molecule formation and thus also the intensity of proteosynthesis was studied in contact surface preparations from tissue samples of 28 patients with epithelial tumors of the bladder and in tissue samples of bladder mucosa from 16 patients with prostata hypertrophy (7 of them suffered also from cystolithiasis). In malignant lesions of the bladder mucosa, especially in tumors of medium and high grade malignancy, very low values of metabolically resting cells with ring shaped nucleoli and micronucleoli were found.", "contents": "Nucleolar morphology in estimation of urinary bladder cancer in man: study on surface contact preparations. The special feature of nucleolar morphology which reflects the intensity of ribosomal RNA precursor molecule formation and thus also the intensity of proteosynthesis was studied in contact surface preparations from tissue samples of 28 patients with epithelial tumors of the bladder and in tissue samples of bladder mucosa from 16 patients with prostata hypertrophy (7 of them suffered also from cystolithiasis). In malignant lesions of the bladder mucosa, especially in tumors of medium and high grade malignancy, very low values of metabolically resting cells with ring shaped nucleoli and micronucleoli were found."}
{"id": "PMID:471128", "title": "Histologic changes in the human skin melanoma after intratumorous treatment with BCG.", "content": "Twenty six patients with malignant skin melanomas were treated with an intratumorous injection of 0.1 ml BCG applicated in certain time intervals. Histologic changes in tumor tissue starting with dystrophy and necrosis of the cells and ending with the formation of a granular tissue replacing the tumor are described. In one case the untreated satellite localized 2 cm from the injected tumor disappeared. The results indicate that the reaction of the cells on the applicated vaccine varied. In some tumors dystrophic changes and necroses were very large, in others they were of a lesser extent, but in all the process ended with the formation of a granular tissue. The mechanism of the action of BCG is discussed. For the time being, it seems that the intratumorous application of BCG is a useful adjuvant therapy.", "contents": "Histologic changes in the human skin melanoma after intratumorous treatment with BCG. Twenty six patients with malignant skin melanomas were treated with an intratumorous injection of 0.1 ml BCG applicated in certain time intervals. Histologic changes in tumor tissue starting with dystrophy and necrosis of the cells and ending with the formation of a granular tissue replacing the tumor are described. In one case the untreated satellite localized 2 cm from the injected tumor disappeared. The results indicate that the reaction of the cells on the applicated vaccine varied. In some tumors dystrophic changes and necroses were very large, in others they were of a lesser extent, but in all the process ended with the formation of a granular tissue. The mechanism of the action of BCG is discussed. For the time being, it seems that the intratumorous application of BCG is a useful adjuvant therapy."}
{"id": "PMID:471129", "title": "Comparative porphyrin content in tumors with contiguous non-neoplastic tissues.", "content": "The porphyrin content in primary human carcinomas, their metastases and the contiguous non-neoplastic tissues were compared. The level of porphyrins in primary internal organ tumors was greater, and in endocrine gland tumors lesser, than in their contiguous non-neoplastic tissues. Neoplastic metastases to the liver contained less porphyrin than the host tissue. Metastases both to the liver and to the kidney were characterized by higher pyrrole pigment content than that in the primary tumors.", "contents": "Comparative porphyrin content in tumors with contiguous non-neoplastic tissues. The porphyrin content in primary human carcinomas, their metastases and the contiguous non-neoplastic tissues were compared. The level of porphyrins in primary internal organ tumors was greater, and in endocrine gland tumors lesser, than in their contiguous non-neoplastic tissues. Neoplastic metastases to the liver contained less porphyrin than the host tissue. Metastases both to the liver and to the kidney were characterized by higher pyrrole pigment content than that in the primary tumors."}
{"id": "PMID:471139", "title": "A beneficial effect of the in situ kidney on in vitro marrow erythropoiesis in chronic renal failure.", "content": "The effect of the in situ kidney on transfusion requirements and in vitro erythropoiesis was investigated in 20 patients with end stage renal disease undergoing hemodialysis. 6 of the 12 patients with in situ kidneys did not require transfusion, whereas the other 6 had an average monthly transfusion requirement of 277 ml of sedimented RBCs. All 8 anephric patients required transfusions with an average requirement of 352 ml of sedimented RBCs per month. Serum erythropoietin activity was inappropriately low for the degree of anemia in all but 1 patient, and bone marrow was uniformly hypocellular. Marrow cells from patients with in situ kidneys exhibited a greater response to erythropoietin than marrow cells from their anephric counterparts. The response was not improved by hemodialysis.", "contents": "A beneficial effect of the in situ kidney on in vitro marrow erythropoiesis in chronic renal failure. The effect of the in situ kidney on transfusion requirements and in vitro erythropoiesis was investigated in 20 patients with end stage renal disease undergoing hemodialysis. 6 of the 12 patients with in situ kidneys did not require transfusion, whereas the other 6 had an average monthly transfusion requirement of 277 ml of sedimented RBCs. All 8 anephric patients required transfusions with an average requirement of 352 ml of sedimented RBCs per month. Serum erythropoietin activity was inappropriately low for the degree of anemia in all but 1 patient, and bone marrow was uniformly hypocellular. Marrow cells from patients with in situ kidneys exhibited a greater response to erythropoietin than marrow cells from their anephric counterparts. The response was not improved by hemodialysis."}
{"id": "PMID:471141", "title": "Therapy of iron deficiency anemia in patients on maintenance dialysis.", "content": "A controlled, prospective study compared the effectiveness of oral ferrous sulfate to intravenous iron dextran, each with and without concurrent intramuscular androgen for therapy of iron deficiency anemia in patients with chronic renal failure treated with maintenance hemodialysis. During the 12-week period of therapy, the patients who received oral ferrous sulfate and androgens showed an increment in their mean hematocrit of 16.3% and those who received oral ferrous sulfate alone had an increase of 8.3%. Patients treated with intravenous iron dextran androgens showed an increment in their mean hematocrit of 9.4% and those given iron dextran alone showed an increase of 3.5%. Serum ferritin levels increased with iron repletion but correlated inversely with the erythropoietic response. The serum ferritin assay provides a simple and reliable method to demonstrate iron repletion, and oral ferrous sulfate is the preferred method of iron repletion in compliant patients.", "contents": "Therapy of iron deficiency anemia in patients on maintenance dialysis. A controlled, prospective study compared the effectiveness of oral ferrous sulfate to intravenous iron dextran, each with and without concurrent intramuscular androgen for therapy of iron deficiency anemia in patients with chronic renal failure treated with maintenance hemodialysis. During the 12-week period of therapy, the patients who received oral ferrous sulfate and androgens showed an increment in their mean hematocrit of 16.3% and those who received oral ferrous sulfate alone had an increase of 8.3%. Patients treated with intravenous iron dextran androgens showed an increment in their mean hematocrit of 9.4% and those given iron dextran alone showed an increase of 3.5%. Serum ferritin levels increased with iron repletion but correlated inversely with the erythropoietic response. The serum ferritin assay provides a simple and reliable method to demonstrate iron repletion, and oral ferrous sulfate is the preferred method of iron repletion in compliant patients."}
{"id": "PMID:471142", "title": "Minimal intermittent heparinization during hemodialysis.", "content": "Minimal dose heparinization inhibiting clotting factor IXa, Xa, XIa, as monitored by the activated partial thromboplastin time, was compared with conventional intermittent, continuous and regional heparinization during hemodialysis treatment. Blood loss in coil dialyzers was the same. Heparin dosage was reduced markedly. Protamine sulfate and infusion equipment were not required. No bleeding problems were encountered in high-risk patients.", "contents": "Minimal intermittent heparinization during hemodialysis. Minimal dose heparinization inhibiting clotting factor IXa, Xa, XIa, as monitored by the activated partial thromboplastin time, was compared with conventional intermittent, continuous and regional heparinization during hemodialysis treatment. Blood loss in coil dialyzers was the same. Heparin dosage was reduced markedly. Protamine sulfate and infusion equipment were not required. No bleeding problems were encountered in high-risk patients."}
{"id": "PMID:471143", "title": "A study of the renal countercurrent system by computer simulation.", "content": "A model of the renal medulla is formulated, taking into account the transport properties of each medullary structure. Using known data on inflow to the vascular and tubular systems and on urine output in normal man in hydropenia, and assuming medullary osmotic profiles similar to published data on hydropenic dogs, transport coefficients are calculated for each medullary structure. The calculated coefficients compare reasonably well with measurements made in the rat kidney. A normal hydration case is calculated by reducing the collecting duct permeability until a urine output and concentration of 1.0ml/min and 700 mosm/l is obtained. The coefficients are then kept constant, and medullary osmotic profiles and urine output corresponding to various system imputs are calculated. These studies show that medullary osmolarities decrease and urine flow increases with increasing medullary blood flow and that an optimal rate of proximal reabsorption exists for producing a maximally concentrated urine. Such results are consistent with observations on renal function reported both experimentally and clinically.", "contents": "A study of the renal countercurrent system by computer simulation. A model of the renal medulla is formulated, taking into account the transport properties of each medullary structure. Using known data on inflow to the vascular and tubular systems and on urine output in normal man in hydropenia, and assuming medullary osmotic profiles similar to published data on hydropenic dogs, transport coefficients are calculated for each medullary structure. The calculated coefficients compare reasonably well with measurements made in the rat kidney. A normal hydration case is calculated by reducing the collecting duct permeability until a urine output and concentration of 1.0ml/min and 700 mosm/l is obtained. The coefficients are then kept constant, and medullary osmotic profiles and urine output corresponding to various system imputs are calculated. These studies show that medullary osmolarities decrease and urine flow increases with increasing medullary blood flow and that an optimal rate of proximal reabsorption exists for producing a maximally concentrated urine. Such results are consistent with observations on renal function reported both experimentally and clinically."}
{"id": "PMID:471144", "title": "Renal tubular necrosis following cephalothin.", "content": "Renal tubular necrosis was observed following intramuscular injections of cephalothin into rats. Lesions were consistently produced with 5.0g/kg and were maximal in severity at the 2nd and 3rd days following injection. Renal tubular necrosis following cephalothin was similar in morphology to that produced by nephrotoxic doses (2.0 g/kg) of cephaloridine. The nephrotoxic potential of cephalothin has been demonstrated in the rat model and caution is urged in using large doses of cephalothin.", "contents": "Renal tubular necrosis following cephalothin. Renal tubular necrosis was observed following intramuscular injections of cephalothin into rats. Lesions were consistently produced with 5.0g/kg and were maximal in severity at the 2nd and 3rd days following injection. Renal tubular necrosis following cephalothin was similar in morphology to that produced by nephrotoxic doses (2.0 g/kg) of cephaloridine. The nephrotoxic potential of cephalothin has been demonstrated in the rat model and caution is urged in using large doses of cephalothin."}
{"id": "PMID:471147", "title": "Renal sites of natriuretic and uricosuric activity of ticrynafen in the mongrel dog.", "content": "Following establishment of steady state plasma concentrations of ticrynafen in the mongrel dog, the intravenous injection of large doses of p-aminohippurate (PAH) or sodium salicylate reduced or blocked the urinary excretion of ticrynafen. In a similar manner, the intravenous administration of ticrynafen reduced the urinary excretion of PAH in preloaded dogs. Since PAH and salicylate are actively secreted by a renal tubular organic anion transport system, these data were interpreted as evidence of an active tubular secretion of ticrynafen. The natriuresis and uricosuria which result from the administration of ticrynafen to the mongrel dog were reduced by PAH and salicylate at doses of these compounds which effectively blocked the secretion of ticrynafen. These results demonstrate that in the dog, the natriuretic and uricosuric activity of ticrynafen results from the presence of ticrynafen in the tubular lumen.", "contents": "Renal sites of natriuretic and uricosuric activity of ticrynafen in the mongrel dog. Following establishment of steady state plasma concentrations of ticrynafen in the mongrel dog, the intravenous injection of large doses of p-aminohippurate (PAH) or sodium salicylate reduced or blocked the urinary excretion of ticrynafen. In a similar manner, the intravenous administration of ticrynafen reduced the urinary excretion of PAH in preloaded dogs. Since PAH and salicylate are actively secreted by a renal tubular organic anion transport system, these data were interpreted as evidence of an active tubular secretion of ticrynafen. The natriuresis and uricosuria which result from the administration of ticrynafen to the mongrel dog were reduced by PAH and salicylate at doses of these compounds which effectively blocked the secretion of ticrynafen. These results demonstrate that in the dog, the natriuretic and uricosuric activity of ticrynafen results from the presence of ticrynafen in the tubular lumen."}
{"id": "PMID:471148", "title": "Effects of MK-196 on urate and electrolyte excretion in the rat.", "content": "Clearance and micropuncture techniques were used to evaluate the effects of MK-196 on uric acid and electrolyte excretion by the rat kidney. The urinary excretion of sodium, uric acid, calcium and magnesium increased significantly following MK-196 administration. The major site of action with respect to sodium reabsorption was in the ascending limb of Henle's loop as revealed by depression of both free-water clearance and reabsorption. By contrast, microinjection studies with [2-14C]-urate revealed the major site of altered urate absorption to be in the proximal convoluted tubule, a site where sodium and water reabsorption was unchanged.", "contents": "Effects of MK-196 on urate and electrolyte excretion in the rat. Clearance and micropuncture techniques were used to evaluate the effects of MK-196 on uric acid and electrolyte excretion by the rat kidney. The urinary excretion of sodium, uric acid, calcium and magnesium increased significantly following MK-196 administration. The major site of action with respect to sodium reabsorption was in the ascending limb of Henle's loop as revealed by depression of both free-water clearance and reabsorption. By contrast, microinjection studies with [2-14C]-urate revealed the major site of altered urate absorption to be in the proximal convoluted tubule, a site where sodium and water reabsorption was unchanged."}
{"id": "PMID:471149", "title": "Ticrynafen: site of natriuretic activity.", "content": "Studies were performed with ticrynafen (tienilic acid) to determine its natriuretic site of action, detailed electrolyte excretion, and its effect on acid excretion and bicarbonate reabsorption. With 500 and 1,000 mg oral doses under conditions of water diuresis, there was a decrease in free water excretion as osmolar clearance increased. During hydropenia, there was no change in free-water reabsorption as osmolar clearance increased. Maximum sodium excretion reached 5.2% of the filtered load. At 500 mg doses, there was no effect on phosphate excretion; with 1,000 mg doses, there was a reduction in phosphate excretion. During bicarbonate infusion, there was no change in the absolute or percent reabsorption of bicarbonate. After chronic administration of ticrynafen, there was no impairment of excretion of al acute acid load. Uric acid excretion increased at least threefold in all studies. The studies indicate that at 500 and 1,000 mg oral doses, ticrynafen has the characteristics of a diuretic which is active in the cortical diluting segment of the distal nephron.", "contents": "Ticrynafen: site of natriuretic activity. Studies were performed with ticrynafen (tienilic acid) to determine its natriuretic site of action, detailed electrolyte excretion, and its effect on acid excretion and bicarbonate reabsorption. With 500 and 1,000 mg oral doses under conditions of water diuresis, there was a decrease in free water excretion as osmolar clearance increased. During hydropenia, there was no change in free-water reabsorption as osmolar clearance increased. Maximum sodium excretion reached 5.2% of the filtered load. At 500 mg doses, there was no effect on phosphate excretion; with 1,000 mg doses, there was a reduction in phosphate excretion. During bicarbonate infusion, there was no change in the absolute or percent reabsorption of bicarbonate. After chronic administration of ticrynafen, there was no impairment of excretion of al acute acid load. Uric acid excretion increased at least threefold in all studies. The studies indicate that at 500 and 1,000 mg oral doses, ticrynafen has the characteristics of a diuretic which is active in the cortical diluting segment of the distal nephron."}
{"id": "PMID:471150", "title": "Elevated serum uric acid. A cardiovascular risk factor?", "content": "A critical survey of the literature suggests that elevated serum uric acid is definitely associated with hypertension and may function as an independent risk factor in the development of this disease. The possible role of an elevated serum uric acid as an independent risk factor for the development of ischemic heart disease remains controversial and the association of these two factors may depend on the relationship between uric acid and hypertension. There is no evidence available to define the metabolic turnover of uric acid in hypertensives. The exact mechanisms underlying elevated serum uric acid values during diuretic treatment are unclear, but depend initially upon diuretic-induced extracellular volume depletion.", "contents": "Elevated serum uric acid. A cardiovascular risk factor? A critical survey of the literature suggests that elevated serum uric acid is definitely associated with hypertension and may function as an independent risk factor in the development of this disease. The possible role of an elevated serum uric acid as an independent risk factor for the development of ischemic heart disease remains controversial and the association of these two factors may depend on the relationship between uric acid and hypertension. There is no evidence available to define the metabolic turnover of uric acid in hypertensives. The exact mechanisms underlying elevated serum uric acid values during diuretic treatment are unclear, but depend initially upon diuretic-induced extracellular volume depletion."}
{"id": "PMID:471151", "title": "Mechanism of the uricosuric activity of ticrynafen.", "content": "Studies employing pyrazinamide, which have included the measurements of clearances of pyrazinamide and pyrazinoate, as well as of ticrynafen itself and its principal metabolite, have lent support to the hypothesis that ticrynafen inhibits the tubular reabsorption of both filtered and secreted urate by nephrons of the human kidney. The inhibition of the reabsorption of secreted urate by ticrynafen appears to be quantitatively important for eliciting its uricosuric and hypouricemic responses. Because the uricosuric and natriuretic responses correlate with urinary ticrynafen concentrations, the drug appears to inhibit tubular reabsorption after gaining access to tubule fluid. As a consequence of its extensive binding to plasma proteins, clearance data suggest that tubular secretion of ticrynafen is necessary for its action.", "contents": "Mechanism of the uricosuric activity of ticrynafen. Studies employing pyrazinamide, which have included the measurements of clearances of pyrazinamide and pyrazinoate, as well as of ticrynafen itself and its principal metabolite, have lent support to the hypothesis that ticrynafen inhibits the tubular reabsorption of both filtered and secreted urate by nephrons of the human kidney. The inhibition of the reabsorption of secreted urate by ticrynafen appears to be quantitatively important for eliciting its uricosuric and hypouricemic responses. Because the uricosuric and natriuretic responses correlate with urinary ticrynafen concentrations, the drug appears to inhibit tubular reabsorption after gaining access to tubule fluid. As a consequence of its extensive binding to plasma proteins, clearance data suggest that tubular secretion of ticrynafen is necessary for its action."}
{"id": "PMID:471152", "title": "Uricosuric response to increasing doses of the indanone diuretic MK-196 in healthy volunteers.", "content": "Doses of 20, 40, 60 and 80 mg of the indanone diuretic MK-196 and 40 mg of frusemide were administered in random order to 5 healthy volunteers in a stable metabolic state, on the same day for 5 consecutive weeks. Diuresis, natriuresis and kaluresis increased with increasing doses, the natriuresis lasting for about 12 h and the kaluresis for about 24 h. Uricosuria increased with increasing doses of MK-196 and lasted for about 6 h. The uricosuria was followed by urate retention, so that there was little net change in the renal excretion of urate. Significant weight loss occurred, so that there was probably some associated plasma volume contraction, which would promote urate retention. By contrast, frusemide caused a reduction in renal excretion of urate at all times.", "contents": "Uricosuric response to increasing doses of the indanone diuretic MK-196 in healthy volunteers. Doses of 20, 40, 60 and 80 mg of the indanone diuretic MK-196 and 40 mg of frusemide were administered in random order to 5 healthy volunteers in a stable metabolic state, on the same day for 5 consecutive weeks. Diuresis, natriuresis and kaluresis increased with increasing doses, the natriuresis lasting for about 12 h and the kaluresis for about 24 h. Uricosuria increased with increasing doses of MK-196 and lasted for about 6 h. The uricosuria was followed by urate retention, so that there was little net change in the renal excretion of urate. Significant weight loss occurred, so that there was probably some associated plasma volume contraction, which would promote urate retention. By contrast, frusemide caused a reduction in renal excretion of urate at all times."}
{"id": "PMID:471153", "title": "Comparative effects of ticrynafen and hydrochlorothiazide on blood pressure, renal function, serum uric acid and electrolytes, and body fluid spaces in hypertensive man.", "content": "25 hypertensive subjects underwent a double-blind, randomized study to compare the effects of ticrynafen (TCNF) with hydrochlorothiazide (HCTZ) on blood pressure (BP), renal function, serum uric acid (UA) and electrolytes, and body fluid spaces. BP was similarly lowered by TCNF and HCTZ. Neither TCNF nor HCTZ had a significant effect on true or regular creatinine, inulin, or PAH clearances. TCNF lowered UA, whereas HCTZ elevated UA. Both produced similar electrolyte disturbances. Neither TCNF nor HCTZ, according to our methods, had a significant effect on plasma volume, extracellular fluid, or total body water. The primary advantage of TCNF compared with HCTZ is its profound hypouricemic effect.", "contents": "Comparative effects of ticrynafen and hydrochlorothiazide on blood pressure, renal function, serum uric acid and electrolytes, and body fluid spaces in hypertensive man. 25 hypertensive subjects underwent a double-blind, randomized study to compare the effects of ticrynafen (TCNF) with hydrochlorothiazide (HCTZ) on blood pressure (BP), renal function, serum uric acid (UA) and electrolytes, and body fluid spaces. BP was similarly lowered by TCNF and HCTZ. Neither TCNF nor HCTZ had a significant effect on true or regular creatinine, inulin, or PAH clearances. TCNF lowered UA, whereas HCTZ elevated UA. Both produced similar electrolyte disturbances. Neither TCNF nor HCTZ, according to our methods, had a significant effect on plasma volume, extracellular fluid, or total body water. The primary advantage of TCNF compared with HCTZ is its profound hypouricemic effect."}
{"id": "PMID:471155", "title": "[Importance of dynamics of scintigraphic changes in the brain for prognosis in stroke].", "content": "The results of scintigraphic investigations of the brain were analysed in patients with ischaemic strokes. In all cases scintigraphic investigation was done twice at least. Particular attention is called to the dynamic development of changes in the size of pathological foci in relation to the degree of improvement in the neurological condition of the patients over one year of follow up. Among patients with considerable neurological improvement control investigations carried out after 4 to 8 weeks of the disease demonstrated return of normal scintigraphic findings or a considerable reduction in the size of pathological foci. On the other hand, in patients without neurological improvement pathological scintigraphic changes persisted much longer, they were present in the investigations performed after 4 to 8 weeks, and even after 3--4 months. The presented observations point out that scintigraphic investigations of the brain can have a prognostic significance for the prognosis in brain strokes. These investigations must be done, however, at least twice between the 2nd and 3rd weeks and between the 4th and 8th weeks of the disease. Normalization of scintigraphic findings or a considerable reduction in the size of the pathological changes in the second investigation may suggest good prognosis.", "contents": "[Importance of dynamics of scintigraphic changes in the brain for prognosis in stroke]. The results of scintigraphic investigations of the brain were analysed in patients with ischaemic strokes. In all cases scintigraphic investigation was done twice at least. Particular attention is called to the dynamic development of changes in the size of pathological foci in relation to the degree of improvement in the neurological condition of the patients over one year of follow up. Among patients with considerable neurological improvement control investigations carried out after 4 to 8 weeks of the disease demonstrated return of normal scintigraphic findings or a considerable reduction in the size of pathological foci. On the other hand, in patients without neurological improvement pathological scintigraphic changes persisted much longer, they were present in the investigations performed after 4 to 8 weeks, and even after 3--4 months. The presented observations point out that scintigraphic investigations of the brain can have a prognostic significance for the prognosis in brain strokes. These investigations must be done, however, at least twice between the 2nd and 3rd weeks and between the 4th and 8th weeks of the disease. Normalization of scintigraphic findings or a considerable reduction in the size of the pathological changes in the second investigation may suggest good prognosis."}
{"id": "PMID:471156", "title": "[Differences in basic rheoencephalographic studies in healthy persons depending on age].", "content": "The method of rheoencephalography (reg) was applied for assessing brain circulation in 40 healthy subjects divided into two age group. The age in the first group was from 20 to 45 years (mean 34.3), in the second group from 46 to 73 years (mean 52.3). Reg records were assessed visually calculating the pertinent indices and arithmetical means of 10 waves on each side. The obtained results point to reduced elasticity of blood vessels in the older age group.", "contents": "[Differences in basic rheoencephalographic studies in healthy persons depending on age]. The method of rheoencephalography (reg) was applied for assessing brain circulation in 40 healthy subjects divided into two age group. The age in the first group was from 20 to 45 years (mean 34.3), in the second group from 46 to 73 years (mean 52.3). Reg records were assessed visually calculating the pertinent indices and arithmetical means of 10 waves on each side. The obtained results point to reduced elasticity of blood vessels in the older age group."}
{"id": "PMID:471157", "title": "[Leukergy in multiple sclerosis before and after treatment with sodium salicylate and dexamethasone (preliminary report)].", "content": "The authors determined the leukergic reaction in the blood of multiple sclerosis patients treated with dexamethasone and sodium salicylate. The investigations were done in two groups of 25 patients each. Dexamethasone was used in a daily dose of 4 mg, and sodium salicylate in a dose of 2.0 g. A significant effect of dexamethasone on reduction of the leukergic forms of leucocyte in the peripheral blood of these patients was observed. There was no significant effect of sodium salicylate on leukergy.", "contents": "[Leukergy in multiple sclerosis before and after treatment with sodium salicylate and dexamethasone (preliminary report)]. The authors determined the leukergic reaction in the blood of multiple sclerosis patients treated with dexamethasone and sodium salicylate. The investigations were done in two groups of 25 patients each. Dexamethasone was used in a daily dose of 4 mg, and sodium salicylate in a dose of 2.0 g. A significant effect of dexamethasone on reduction of the leukergic forms of leucocyte in the peripheral blood of these patients was observed. There was no significant effect of sodium salicylate on leukergy."}
{"id": "PMID:471159", "title": "[Pneumoencephalographic appearance of cerebral ventricles in spontaneous tremor].", "content": "Three patients of different ages with evidence of essential tremor of familial origin are reported. Pneumoencephalography demonstrated in these cases presence of hydrocephalus. The size of the cerebral ventricles was assessed by means of the index of Schiermann and Evans. No correlation was observed between the degree of changes in the ventricular system and the duration of the disease. Abnormalities moencephalograms concerned only the Evans index.", "contents": "[Pneumoencephalographic appearance of cerebral ventricles in spontaneous tremor]. Three patients of different ages with evidence of essential tremor of familial origin are reported. Pneumoencephalography demonstrated in these cases presence of hydrocephalus. The size of the cerebral ventricles was assessed by means of the index of Schiermann and Evans. No correlation was observed between the degree of changes in the ventricular system and the duration of the disease. Abnormalities moencephalograms concerned only the Evans index."}
{"id": "PMID:471158", "title": "[Analysis of changes in the incidence of seizures in pregnant women with epilepsy].", "content": "Sixty-five (41%) of 153 pregnant women with epilepsy were selected for analysis because the course of the disease changed in them during pregnancy. In 43 of these women (67%) the frequency of seizures decreased and in 21 cases the seizures disappeared completely. In 22 patients (33%) the frequency of seizures increased in pregnancy. An unfavourable influence of pregnancy was observed more frequently in patients with vourable influence of pregnancy was observed more frequently in patients with severe epilepsy with evidence of organic changes in the central nervous system and mental changes. The prognosis was better in women with less severe disease continuing anticonvulsant treatment.", "contents": "[Analysis of changes in the incidence of seizures in pregnant women with epilepsy]. Sixty-five (41%) of 153 pregnant women with epilepsy were selected for analysis because the course of the disease changed in them during pregnancy. In 43 of these women (67%) the frequency of seizures decreased and in 21 cases the seizures disappeared completely. In 22 patients (33%) the frequency of seizures increased in pregnancy. An unfavourable influence of pregnancy was observed more frequently in patients with vourable influence of pregnancy was observed more frequently in patients with severe epilepsy with evidence of organic changes in the central nervous system and mental changes. The prognosis was better in women with less severe disease continuing anticonvulsant treatment."}
{"id": "PMID:471160", "title": "[Comparison of the changes in bioelectric activity recorded simultaneously from the amygdaloid body and the hippocampus and from the cranial surface during the stereoelectroencephalographic examination in epilepsy].", "content": "The authors analysed 95 SEEG records in epileptics with prevalent temporal-lobe changes comparing the bioelectric activity of the brain recorded simultaneously in the amygdala, hippocampus, temporal cortex and cranial surface during these investigations and relating them to EEG records obtained before the operation. In these records pathological elements typical of epilepsy were found occurring in different variants synchronously or asynchronously in leads at three above mentioned levels. In the findings reported attention is called to occurrence of seizure discharges in the amygdala and hippocampus which had no equivalent in cortical or cranial bioactivity records. In the light of these SEEG observations and a survey of the literature the authors stress the diagnostic importance of SEEG investigations in epilepsy.", "contents": "[Comparison of the changes in bioelectric activity recorded simultaneously from the amygdaloid body and the hippocampus and from the cranial surface during the stereoelectroencephalographic examination in epilepsy]. The authors analysed 95 SEEG records in epileptics with prevalent temporal-lobe changes comparing the bioelectric activity of the brain recorded simultaneously in the amygdala, hippocampus, temporal cortex and cranial surface during these investigations and relating them to EEG records obtained before the operation. In these records pathological elements typical of epilepsy were found occurring in different variants synchronously or asynchronously in leads at three above mentioned levels. In the findings reported attention is called to occurrence of seizure discharges in the amygdala and hippocampus which had no equivalent in cortical or cranial bioactivity records. In the light of these SEEG observations and a survey of the literature the authors stress the diagnostic importance of SEEG investigations in epilepsy."}
{"id": "PMID:471161", "title": "[Disorders of dopamine and 5-hydroxytryptamine metabolism in the early period after cranio-cerebral injuries].", "content": "In patients with signs of intracranial hypertension caused by subdural haematoma or brain oedema following craniocerebral trauma significantly increased concentrations of HVA and 5-HIAA were found in the cerebrospinal fluid obtained by means of lumbar punction in relation to a control group comprising patients with lumbar disc prolapse. In patients after craniocerebral injuries with open fracture of cranial bones without disturbances of consciousness dopaminergic system hyperactivity was observed lasting up to the 7th day after injury. In patients with symptoms of brain commotion after craniocerebral trauma a significant rise in 5-HIAA concentration was observed in the cerebrospinal fluid on the 7th day after trauma. This may suggest increased serotonin turnover early after trauma.", "contents": "[Disorders of dopamine and 5-hydroxytryptamine metabolism in the early period after cranio-cerebral injuries]. In patients with signs of intracranial hypertension caused by subdural haematoma or brain oedema following craniocerebral trauma significantly increased concentrations of HVA and 5-HIAA were found in the cerebrospinal fluid obtained by means of lumbar punction in relation to a control group comprising patients with lumbar disc prolapse. In patients after craniocerebral injuries with open fracture of cranial bones without disturbances of consciousness dopaminergic system hyperactivity was observed lasting up to the 7th day after injury. In patients with symptoms of brain commotion after craniocerebral trauma a significant rise in 5-HIAA concentration was observed in the cerebrospinal fluid on the 7th day after trauma. This may suggest increased serotonin turnover early after trauma."}
{"id": "PMID:471162", "title": "[Value of the neuropsychological examinations in the diagnosis of supratentorial gliomas].", "content": "The authors report an analysis of the results of neuropsychological investigations of patients treated for supratentorial gliomas. A syndrome of the most frequently observed disturbances of higher nervous activity has been outlined. The diagnostic, localizatlry and differential diagnostic value of neuropsychological investigations has been assessed and found to a useful auxiliary clinical method.", "contents": "[Value of the neuropsychological examinations in the diagnosis of supratentorial gliomas]. The authors report an analysis of the results of neuropsychological investigations of patients treated for supratentorial gliomas. A syndrome of the most frequently observed disturbances of higher nervous activity has been outlined. The diagnostic, localizatlry and differential diagnostic value of neuropsychological investigations has been assessed and found to a useful auxiliary clinical method."}
{"id": "PMID:471169", "title": "[Subarachnoid hemorrhage and circulatory disturbance of cerebrospinal fluid--scanning electron microscopid study in clinical and autopsy cases (author's transl)].", "content": "The scanning electron microscopy (SEM) gives intersting information about the changes of the subarachnoid space. In this study, specimens from 16 patients (one during surgery and others at autopsy) were examined by SEM: 11 cases of subarachnoid hemorrhage, 2 of posterior fossa brain tumor operated and 3 of control cases without CNS diseases. In cases of subarachnoid hemorrhage, there seemed to be three stages of the obstruction in the subarachnoid space: a) obstruction by blood clots mainly consisted of red blood cells, b) obstruction by subarachnoid fibrosis with thickening of arachnoid, c) obstruction by arachnoid adhesion obliterating subarachnoid space. Specimens from parietal parasagittal area, lateral cerebral fissure and temporal base on both sides were systematically examined by SEM, and the degree of the obstructive changes of the subarachnoid space were classified into five grades: 0) no changes 1) minimal changes--slight thickening of arachnoid and perivascular fibrosis in subarachnoid space 2) moderate changes--thickening of arachnoid and fibrosis in subarachnoid space with patent meshwork 3) severe obstruction of subarachnoid meshwork 4) complete obstruction of subarachnoid space--no space for CSF circulation. We found high incidence of communicating hydrocephalus after SAH in those cases in which epicortical CSF circulation was obstructed more than grade 3 in the parasagittal area bilaterally.", "contents": "[Subarachnoid hemorrhage and circulatory disturbance of cerebrospinal fluid--scanning electron microscopid study in clinical and autopsy cases (author's transl)]. The scanning electron microscopy (SEM) gives intersting information about the changes of the subarachnoid space. In this study, specimens from 16 patients (one during surgery and others at autopsy) were examined by SEM: 11 cases of subarachnoid hemorrhage, 2 of posterior fossa brain tumor operated and 3 of control cases without CNS diseases. In cases of subarachnoid hemorrhage, there seemed to be three stages of the obstruction in the subarachnoid space: a) obstruction by blood clots mainly consisted of red blood cells, b) obstruction by subarachnoid fibrosis with thickening of arachnoid, c) obstruction by arachnoid adhesion obliterating subarachnoid space. Specimens from parietal parasagittal area, lateral cerebral fissure and temporal base on both sides were systematically examined by SEM, and the degree of the obstructive changes of the subarachnoid space were classified into five grades: 0) no changes 1) minimal changes--slight thickening of arachnoid and perivascular fibrosis in subarachnoid space 2) moderate changes--thickening of arachnoid and fibrosis in subarachnoid space with patent meshwork 3) severe obstruction of subarachnoid meshwork 4) complete obstruction of subarachnoid space--no space for CSF circulation. We found high incidence of communicating hydrocephalus after SAH in those cases in which epicortical CSF circulation was obstructed more than grade 3 in the parasagittal area bilaterally."}
{"id": "PMID:471170", "title": "[Large abdominal pseudocyst as a complication of ventriculoperitoneal shunt--diagnosis by ultrasonography and whole body CT scan (author's transl)].", "content": "We report a case in which a large abdominal pseudocyst developed following a ventriculo-peritoneal shunt (V-P shunt) for hydrocephalus due to pineal germinoma. A 24-year-old man had undergone a V-P shunt and radiotheraphy for unbiopsied posterior third ventricle tumor. Four years after operation he was readmitted because of double vision, conjugate upward gaze palsy and a large abdominal mass. Preoperative diagnosis of abdominal pseudocyst was made by abdominal computed tomography and ultrasonotomography. The shunt tube was clearly deomonstrated in the cavity by computed tomography. It is suggested that, in a patient suspicious of having abdominal pseudocyst who developed abdominal symptoms and signs following V-P shunt, abdominal computed tomography and/or ultrasonotomography provide a definitive diagnosis of this entity.", "contents": "[Large abdominal pseudocyst as a complication of ventriculoperitoneal shunt--diagnosis by ultrasonography and whole body CT scan (author's transl)]. We report a case in which a large abdominal pseudocyst developed following a ventriculo-peritoneal shunt (V-P shunt) for hydrocephalus due to pineal germinoma. A 24-year-old man had undergone a V-P shunt and radiotheraphy for unbiopsied posterior third ventricle tumor. Four years after operation he was readmitted because of double vision, conjugate upward gaze palsy and a large abdominal mass. Preoperative diagnosis of abdominal pseudocyst was made by abdominal computed tomography and ultrasonotomography. The shunt tube was clearly deomonstrated in the cavity by computed tomography. It is suggested that, in a patient suspicious of having abdominal pseudocyst who developed abdominal symptoms and signs following V-P shunt, abdominal computed tomography and/or ultrasonotomography provide a definitive diagnosis of this entity."}
{"id": "PMID:471171", "title": "[Two cases of azygos anterior cerebral artery aneurysm (author's transl)].", "content": "Two cases with azygos anterior cerebral artery were reported from an analysis of 37 cases of distal anterior cerebral artery aneurysm. Case 1 was a 57-year-old woman. She had an attack of subarachnoid hemorrhage two months before admission to our clinic. The anterior cerebral artery was not demonstrated on the right carotid angiogram, and an azygos anterior cerebral artery was visualized on the left carotid angiogram. The aneurysm was situated at the distal end of the azygos artery. Case 2 was a 71-year-old hypertensive woman. Subarachnoid hemorrhage occurred 6 days before admission to our clinic. The left carotid angiography with contraleteral compression revealed an azygos artery and an aneurysm in its middle part. The azygos arteries in both cases were confirmed at operation and aneurysmal necks were managed without any serious deficit. The distal anterior cerebral artery aneurysm is frequently accompanied by azygos artery. This vascular anomaly may cause a hemodynamic change and may be one of the factors of aneurysmal formation at this part.", "contents": "[Two cases of azygos anterior cerebral artery aneurysm (author's transl)]. Two cases with azygos anterior cerebral artery were reported from an analysis of 37 cases of distal anterior cerebral artery aneurysm. Case 1 was a 57-year-old woman. She had an attack of subarachnoid hemorrhage two months before admission to our clinic. The anterior cerebral artery was not demonstrated on the right carotid angiogram, and an azygos anterior cerebral artery was visualized on the left carotid angiogram. The aneurysm was situated at the distal end of the azygos artery. Case 2 was a 71-year-old hypertensive woman. Subarachnoid hemorrhage occurred 6 days before admission to our clinic. The left carotid angiography with contraleteral compression revealed an azygos artery and an aneurysm in its middle part. The azygos arteries in both cases were confirmed at operation and aneurysmal necks were managed without any serious deficit. The distal anterior cerebral artery aneurysm is frequently accompanied by azygos artery. This vascular anomaly may cause a hemodynamic change and may be one of the factors of aneurysmal formation at this part."}
{"id": "PMID:471165", "title": "[Central prolapse of the Th12 -L1 nucleus pulposus with radiological signs of tumor of the conus medullaris].", "content": "The authors report a 42-year-old patient with excruciating pains felt in the interscapular and lumbar regions and both lower extremities without symptoms of root involvement. Myelography suggested presence of a tumour of the conus medullaris or epidural tumour in this area, at the Th12--L1 level. During the operation a central prolapse of the nucleus pulposus was found in the midline on the anterior wall of the vertebral canal below the longitudinal ligament. After operation all symptoms disappeared and the patient resumed work.", "contents": "[Central prolapse of the Th12 -L1 nucleus pulposus with radiological signs of tumor of the conus medullaris]. The authors report a 42-year-old patient with excruciating pains felt in the interscapular and lumbar regions and both lower extremities without symptoms of root involvement. Myelography suggested presence of a tumour of the conus medullaris or epidural tumour in this area, at the Th12--L1 level. During the operation a central prolapse of the nucleus pulposus was found in the midline on the anterior wall of the vertebral canal below the longitudinal ligament. After operation all symptoms disappeared and the patient resumed work."}
{"id": "PMID:471173", "title": "[A case of congenital dermal sinus in the parietal region (author's transl)].", "content": "A congenital dermal sinus at the midline of the parietal region, which extends into the falx, is relatively rare. The following case is illustrative. A 3-year-old girl was noticed by her parents to have tumor at the midline of the parietal region without other disturbances and complaints. This girl had the normal mental and motor development, and was admitted to our clinic for cosmetic surgery. A round, elastic soft tumor, roughly 1x1x1 cm in diameter, was located at the midline of the parietal region. This tumor had a port wine cutaneous discoloration and an absence of normal hair growth in this area. Abnormal hair did not protrude from the sinus. This tumor had no dimple nor sinus opening. Skull XP showed a round lucent bone defect at the midline of the parietal region. This bone defect with sclerotic margin, was about 1 cm in diameter. Skull XP and tomography, with contrast medium injection into the tumor, showed that this tumor extended into the falx through bone defect. Angiography showed that there were no relations between the tumor and the superior sagittal sinus. PEG showed that there were no mass lesion nor dilatation of ventricles. Both spinal tap and EEG were normal. Operation for the congenital dermal sinus was performed on Nov. 4, 1977. This congenital dermal sinus extended into the falx and congenital cell rest tumor was not elicited.", "contents": "[A case of congenital dermal sinus in the parietal region (author's transl)]. A congenital dermal sinus at the midline of the parietal region, which extends into the falx, is relatively rare. The following case is illustrative. A 3-year-old girl was noticed by her parents to have tumor at the midline of the parietal region without other disturbances and complaints. This girl had the normal mental and motor development, and was admitted to our clinic for cosmetic surgery. A round, elastic soft tumor, roughly 1x1x1 cm in diameter, was located at the midline of the parietal region. This tumor had a port wine cutaneous discoloration and an absence of normal hair growth in this area. Abnormal hair did not protrude from the sinus. This tumor had no dimple nor sinus opening. Skull XP showed a round lucent bone defect at the midline of the parietal region. This bone defect with sclerotic margin, was about 1 cm in diameter. Skull XP and tomography, with contrast medium injection into the tumor, showed that this tumor extended into the falx through bone defect. Angiography showed that there were no relations between the tumor and the superior sagittal sinus. PEG showed that there were no mass lesion nor dilatation of ventricles. Both spinal tap and EEG were normal. Operation for the congenital dermal sinus was performed on Nov. 4, 1977. This congenital dermal sinus extended into the falx and congenital cell rest tumor was not elicited."}
{"id": "PMID:471179", "title": "[A case with giant aneurysm of distal portion of the posterior inferior cerebellar artery (author's transl)].", "content": "A case of a giant aneurysm of the distal posterior inferior cerebellar artery has been presented, in which the preoperative sympotoms were those of a space occupying lesion of the posterior fossa. CT scan revealed a round mass in the cerebellum. The wall of this round mass was not enhanced after an intravenous administration of iodine. At operation we found a large saccular aneurysm, about 3 cm in diameter, arising from the distal portion of the left posterior inferior cerebellar artery. By needle decompression and dissection around the aneurysm, we were able to place a Heifetz clip across the neck. After exicision, the aneurysm was found to contain a large thrombus.", "contents": "[A case with giant aneurysm of distal portion of the posterior inferior cerebellar artery (author's transl)]. A case of a giant aneurysm of the distal posterior inferior cerebellar artery has been presented, in which the preoperative sympotoms were those of a space occupying lesion of the posterior fossa. CT scan revealed a round mass in the cerebellum. The wall of this round mass was not enhanced after an intravenous administration of iodine. At operation we found a large saccular aneurysm, about 3 cm in diameter, arising from the distal portion of the left posterior inferior cerebellar artery. By needle decompression and dissection around the aneurysm, we were able to place a Heifetz clip across the neck. After exicision, the aneurysm was found to contain a large thrombus."}
{"id": "PMID:471182", "title": "[A case of choroid plexus angioma with subarachnoid hemorrhage--case report (author's transl)].", "content": "Authors report a case of choroid plexus angioma of lateral ventricle, in a 42 years old woman, initiated with an episode of subarachnoid hemorrhage. The localization of lesion, in the anterior horn and body of right lateral ventricle, producing primary intraventricular bleeding was revealed by pneumoencephalography, especially polytome pneumotomography. However, it was difficult to find by angiography. Patient was successfully removed angioma and showed good recovery. In review of the literatures, the choroid plexus angioma is found more frequently in children than adults, caused by primary intraventricular or subarachnoid hemorrhage and is ocasionally associated with other congenital anomalies. Otherwise, it is found incidentally at autopsy and show hydrocephalus or normal pressure hydrocephalus. Prognosis is excellent in operated case, but heavy mortality in non-operated case and combined with intraventricular hematoma.", "contents": "[A case of choroid plexus angioma with subarachnoid hemorrhage--case report (author's transl)]. Authors report a case of choroid plexus angioma of lateral ventricle, in a 42 years old woman, initiated with an episode of subarachnoid hemorrhage. The localization of lesion, in the anterior horn and body of right lateral ventricle, producing primary intraventricular bleeding was revealed by pneumoencephalography, especially polytome pneumotomography. However, it was difficult to find by angiography. Patient was successfully removed angioma and showed good recovery. In review of the literatures, the choroid plexus angioma is found more frequently in children than adults, caused by primary intraventricular or subarachnoid hemorrhage and is ocasionally associated with other congenital anomalies. Otherwise, it is found incidentally at autopsy and show hydrocephalus or normal pressure hydrocephalus. Prognosis is excellent in operated case, but heavy mortality in non-operated case and combined with intraventricular hematoma."}
{"id": "PMID:471183", "title": "Foetal brain development in the sheep.", "content": "The pattern of normal foetal brain development has been described for the Merino sheep. Controlled flock matings were used and foetuses removed by hysterotomy at 40, 54, 67, 81, 90, 95, 109, 121 and 150 days of gestation. Lambs at 7 days after birth and adults were also studied. The CNS was dissected, separated and weighed as four segments--cerebral hemispheres, brain stem, cerebellum and spinal cord. Determinations of DNA, RNA, protein, cholesterol and water content were subsequently carried out on each segment. Brain weight increased from 0.264 g (40 days) to 52.74 g at 150 days. The ratio to body weight decreased from 6.7% (40 days) to 1.5% (150 days). DNA increased more rapidly from 40 to 90 days in the cerebral hemispheres and brain stem than in the cerebellum and spinal cord and this increase preceded the main increase in weight. Increases in cholesterol and decreases in water content were also more marked in the cerebral hemispheres and brain stem. Increase in brain weight occurred in two phases, one up to 90 days followed by a more rapid and larger increase after 90 days which continued to birth. These two phases appear to reflect an increase in neuroblast multiplication followed by neuroglial multiplication and myelination respectively. At birth, brain weight had reached 50% of adult size, the cerebral hemispheres 52% of adult size, the cerebellum 40% and spinal cord 40%. The relative maturity of the brain at birth justifies the classification of the sheep as a prenatal brain developer.", "contents": "Foetal brain development in the sheep. The pattern of normal foetal brain development has been described for the Merino sheep. Controlled flock matings were used and foetuses removed by hysterotomy at 40, 54, 67, 81, 90, 95, 109, 121 and 150 days of gestation. Lambs at 7 days after birth and adults were also studied. The CNS was dissected, separated and weighed as four segments--cerebral hemispheres, brain stem, cerebellum and spinal cord. Determinations of DNA, RNA, protein, cholesterol and water content were subsequently carried out on each segment. Brain weight increased from 0.264 g (40 days) to 52.74 g at 150 days. The ratio to body weight decreased from 6.7% (40 days) to 1.5% (150 days). DNA increased more rapidly from 40 to 90 days in the cerebral hemispheres and brain stem than in the cerebellum and spinal cord and this increase preceded the main increase in weight. Increases in cholesterol and decreases in water content were also more marked in the cerebral hemispheres and brain stem. Increase in brain weight occurred in two phases, one up to 90 days followed by a more rapid and larger increase after 90 days which continued to birth. These two phases appear to reflect an increase in neuroblast multiplication followed by neuroglial multiplication and myelination respectively. At birth, brain weight had reached 50% of adult size, the cerebral hemispheres 52% of adult size, the cerebellum 40% and spinal cord 40%. The relative maturity of the brain at birth justifies the classification of the sheep as a prenatal brain developer."}
{"id": "PMID:471184", "title": "The effects of postnatal lead exposure on Purkinje cell dendritic development in the rat.", "content": "Rat pups, suckled by mothers maintained after parturition on a diet containing 4% lead acetate, were killed at 30 days and their cerebella examined. The blood lead was greatly increased in these animals (258.20 +/- 6.72 micrograms/100 ml) as compared with controls (4.75 +/- 0.75 micrograms/100 ml) and their mean body weight was reduced by 28%. Cerebellar weight, however, remained unchanged. Histologically the vermis showed vacuolation of the white matter and an increase in the size of Purkinje cell bodies. The total number of Purkinje and granule cells and their densities were unchanged except in animals with encephalopathy when these parameters were reduced. Network analysis of the dendritic trees of Purkinje cells indicated a 34.8% reduction in total dendritic length, due to reduction in total segment number and in the length of distal segments. Dendritic density and the frequency of trichotomous branching were unchanged by the experimental treatment. The density of dendritic spines over the periphery of the network was normal. The topology of the dendritic trees of Purkinje cells was abnormal in that branching patterns deviated from the normal pattern generated by random terminal growth. These results suggest that lead causes changes in Purkinje cell metabolism which reduce the rate of dendritic growth and cause abnormal branching. It remains to be determined whether these are direct effects or secondary to the vascular changes known to occur in the cerebellum during lead intoxication.", "contents": "The effects of postnatal lead exposure on Purkinje cell dendritic development in the rat. Rat pups, suckled by mothers maintained after parturition on a diet containing 4% lead acetate, were killed at 30 days and their cerebella examined. The blood lead was greatly increased in these animals (258.20 +/- 6.72 micrograms/100 ml) as compared with controls (4.75 +/- 0.75 micrograms/100 ml) and their mean body weight was reduced by 28%. Cerebellar weight, however, remained unchanged. Histologically the vermis showed vacuolation of the white matter and an increase in the size of Purkinje cell bodies. The total number of Purkinje and granule cells and their densities were unchanged except in animals with encephalopathy when these parameters were reduced. Network analysis of the dendritic trees of Purkinje cells indicated a 34.8% reduction in total dendritic length, due to reduction in total segment number and in the length of distal segments. Dendritic density and the frequency of trichotomous branching were unchanged by the experimental treatment. The density of dendritic spines over the periphery of the network was normal. The topology of the dendritic trees of Purkinje cells was abnormal in that branching patterns deviated from the normal pattern generated by random terminal growth. These results suggest that lead causes changes in Purkinje cell metabolism which reduce the rate of dendritic growth and cause abnormal branching. It remains to be determined whether these are direct effects or secondary to the vascular changes known to occur in the cerebellum during lead intoxication."}
{"id": "PMID:471185", "title": "Atypical axon-Schwann cell relationships in the common peroneal nerve of the dystrophic mouse: an ultrastructural study.", "content": "Several atypical features of myelination of the peripheral nervous system are reported in common peroneal nerve of dystrophic mice (129 Re J dy/dy): (i) central nervous system-like contact between myelin sheaths of adjacent nerve fibres; (ii) nodes and internodes of myelinated fibres enwrapped with cytoplasmic processes of Schwann cells from adjacent nerve fibres; (iii) Schwann cells of adjacent nerve fibres co-operating in formation of a single myelin sheath; and (iv) a single Schwann cell myelinating two separate axons. In view of the presence of similar features of myelination in the central nervous system, where the myelin producing cells lack basement membrane, we suggest that in the dystrophic peripheral nerves the development of these features can be attributed to the partial deficiency of the Schwann cell basement membrane. Two types of widened nodes of Ranvier are also identified: (i) nodes with paranodal damage; and (ii) nodes without paranodal damage. In addition, abnormal features of myelination are described which are likely to represent altered Schwann cell/axon relationships during demyelination and remyelination and/or decreased myelinating ability of Schwann cells. We interpret these findings as indicating a metabolic disorder of Schwann cells. They provide an experimental model for the investigation of factors involved in the origin and maintenance of the structural organization of peripheral nerve.", "contents": "Atypical axon-Schwann cell relationships in the common peroneal nerve of the dystrophic mouse: an ultrastructural study. Several atypical features of myelination of the peripheral nervous system are reported in common peroneal nerve of dystrophic mice (129 Re J dy/dy): (i) central nervous system-like contact between myelin sheaths of adjacent nerve fibres; (ii) nodes and internodes of myelinated fibres enwrapped with cytoplasmic processes of Schwann cells from adjacent nerve fibres; (iii) Schwann cells of adjacent nerve fibres co-operating in formation of a single myelin sheath; and (iv) a single Schwann cell myelinating two separate axons. In view of the presence of similar features of myelination in the central nervous system, where the myelin producing cells lack basement membrane, we suggest that in the dystrophic peripheral nerves the development of these features can be attributed to the partial deficiency of the Schwann cell basement membrane. Two types of widened nodes of Ranvier are also identified: (i) nodes with paranodal damage; and (ii) nodes without paranodal damage. In addition, abnormal features of myelination are described which are likely to represent altered Schwann cell/axon relationships during demyelination and remyelination and/or decreased myelinating ability of Schwann cells. We interpret these findings as indicating a metabolic disorder of Schwann cells. They provide an experimental model for the investigation of factors involved in the origin and maintenance of the structural organization of peripheral nerve."}
{"id": "PMID:471186", "title": "Vasoactive intestinal polypeptide in the pituitary pars nervosa.", "content": "Vasoactive intestinal polypeptide (VIP) is a widely distributed neuropeptide which has recently been found in the hypothalamus and in hypothalamo-hypophyseal portal blood. We have examined the pituitary of several mammalian species for the presence of VIP by immunocytochemistry and radioimmunoassay and report here that the pars nervosa contains a considerable quantity of VIP (100-250 fmol/mg) which, in the dog, can be shown to be present in nerve fibres. It is possible that neurohypophyseal VIP may be a local releasing agent for vasopressin.", "contents": "Vasoactive intestinal polypeptide in the pituitary pars nervosa. Vasoactive intestinal polypeptide (VIP) is a widely distributed neuropeptide which has recently been found in the hypothalamus and in hypothalamo-hypophyseal portal blood. We have examined the pituitary of several mammalian species for the presence of VIP by immunocytochemistry and radioimmunoassay and report here that the pars nervosa contains a considerable quantity of VIP (100-250 fmol/mg) which, in the dog, can be shown to be present in nerve fibres. It is possible that neurohypophyseal VIP may be a local releasing agent for vasopressin."}
{"id": "PMID:471187", "title": "Fine structure of muscle in human disuse atrophy: significance of proximal muscle involvement in muscle disorders.", "content": "The universal occurrence of weakness of skeletal musculature on disuse, however produced, and the paucity of published reports on the fine structural changes in human disuse atrophy, prompted the present investigation. The quadriceps muscle of a leg immobilized in plaster cast (for fracture) and of the opposite non-immobilized limb was biopsied in four adult males, after periods of immobilization from 50 to 75 days. These 8 muscle specimens were examined for histopathological changes, and muscle fibre diameters were measured by micrometry from paraffin sections. The histograms revealed a larger proportion of small fibres (less than 20 micron) and a smaller proportion of large fibres (greater than 40 micron) in the immobilized limb compared to the opposite. Thus, light microscopy showed only atrophic changes. This was confirmed by electronmicroscopy, where atrophy of few to several muscle fibres was seen in the form of loss of myofibrils, collapse and folding of the basement membrane and prominence of glycogen or muscle nuclei. The atrophic change was more severe in the immobilized limbs, but it was also noticeable in all the non-immobilized limbs. Degenerative changes, especially disorganization and breakdown of myofibrils, and fragmentation of plasma membrane, were also seen in occasional atrophied muscle fibres, again more frequently in the immobilized limb. Lipofuscin was often found accumulated in muscle fibres and occasionally in endothelial cells of intramuscular blood vessels; the latter showed prominent pinocytotic vesicles or thickened basement membrane. It is concluded that both atrophy and degeneration of fibres of proximal muscles can occur as non-specific consequences of disuse of the limb in man, that degeneration is a latter and more severe change, that muscles even of the non-immobilized leg are subjected to disuse atrophy during bed-rest, and that the proximal muscles in man seem to have a natural susceptibility to atrophy and degeneration in any muscular disorders.", "contents": "Fine structure of muscle in human disuse atrophy: significance of proximal muscle involvement in muscle disorders. The universal occurrence of weakness of skeletal musculature on disuse, however produced, and the paucity of published reports on the fine structural changes in human disuse atrophy, prompted the present investigation. The quadriceps muscle of a leg immobilized in plaster cast (for fracture) and of the opposite non-immobilized limb was biopsied in four adult males, after periods of immobilization from 50 to 75 days. These 8 muscle specimens were examined for histopathological changes, and muscle fibre diameters were measured by micrometry from paraffin sections. The histograms revealed a larger proportion of small fibres (less than 20 micron) and a smaller proportion of large fibres (greater than 40 micron) in the immobilized limb compared to the opposite. Thus, light microscopy showed only atrophic changes. This was confirmed by electronmicroscopy, where atrophy of few to several muscle fibres was seen in the form of loss of myofibrils, collapse and folding of the basement membrane and prominence of glycogen or muscle nuclei. The atrophic change was more severe in the immobilized limbs, but it was also noticeable in all the non-immobilized limbs. Degenerative changes, especially disorganization and breakdown of myofibrils, and fragmentation of plasma membrane, were also seen in occasional atrophied muscle fibres, again more frequently in the immobilized limb. Lipofuscin was often found accumulated in muscle fibres and occasionally in endothelial cells of intramuscular blood vessels; the latter showed prominent pinocytotic vesicles or thickened basement membrane. It is concluded that both atrophy and degeneration of fibres of proximal muscles can occur as non-specific consequences of disuse of the limb in man, that degeneration is a latter and more severe change, that muscles even of the non-immobilized leg are subjected to disuse atrophy during bed-rest, and that the proximal muscles in man seem to have a natural susceptibility to atrophy and degeneration in any muscular disorders."}
{"id": "PMID:471188", "title": "Spinal cord transection: a quantitative analysis of elements of the connective tissue matrix formed within the site of lesion following administration of piromen, cytoxan or trypsin.", "content": "Long-Evans hooded rats were cordotomized at the T-5 level and given either (1) cyclophosphamide (cytoxan), an immunosuppressive, (2) piromen, a bacterial polysaccharide-nucleic acid complex, (3) topical and systemic trypsin, or (4) no further specific treatment. Because of past and present controversy surrounding the proposed ability of these agents to promote spinal cord regeneration, a systematic study, employing light and electron microscopy, and quantitative methods in a single animal model, was done in order to re-evaluate the effects of each treatment upon the connective tissue matrix which forms in the defect left by transection. After an initial inflammatory reaction during the first week after surgery, the lesion zone is characterized either by areas of dense collagenous connective tissue with occasional fibroblasts and macrophages, or a loose areolar tissue with numerous sheets and cords of mesodermal cellular elements but minimal collagen. By 45 days postoperatively (dpo), axons supported by Schwann cells invade and become entangled in the loose connective tissue matrix. With longer postoperative survival, cysts appear craniad and caudad to the lesion and erode much of the scar together with viable neural tissue. Giving cytoxan or piromen did not result in any qualitative alteration of the scar matrix as evidenced by electron microscopy. Quantitative analysis revealed a slight reduction in the fibrous connective tissue component of the scar at 45--90 dpo, but this was transient when longer postoperative periods were studied. Trypsin caused a significant reduction in the amount of fibrous connective tissue with a concomitant increase in loose connective tissue and the appearance of a few distinctive, compact bundles of unmyelinated axons lacking Schwann cells. Consistent behavioral changes were not observed in any group which could distinguish them from the controls. Our results appear to contradict the findings of Matinian and Andreasian (1976) who reported return of normal sensori-motor function in 80% of their animals treated with topical and systemic trypsin. It is concluded that a major impediment to whatever longterm regenerative potential exists within the spinal cord is the lack of axonal guiding elements within the scar, but more importantly, the severe erosion of the remaining spinal cord due to cyst enlargement.", "contents": "Spinal cord transection: a quantitative analysis of elements of the connective tissue matrix formed within the site of lesion following administration of piromen, cytoxan or trypsin. Long-Evans hooded rats were cordotomized at the T-5 level and given either (1) cyclophosphamide (cytoxan), an immunosuppressive, (2) piromen, a bacterial polysaccharide-nucleic acid complex, (3) topical and systemic trypsin, or (4) no further specific treatment. Because of past and present controversy surrounding the proposed ability of these agents to promote spinal cord regeneration, a systematic study, employing light and electron microscopy, and quantitative methods in a single animal model, was done in order to re-evaluate the effects of each treatment upon the connective tissue matrix which forms in the defect left by transection. After an initial inflammatory reaction during the first week after surgery, the lesion zone is characterized either by areas of dense collagenous connective tissue with occasional fibroblasts and macrophages, or a loose areolar tissue with numerous sheets and cords of mesodermal cellular elements but minimal collagen. By 45 days postoperatively (dpo), axons supported by Schwann cells invade and become entangled in the loose connective tissue matrix. With longer postoperative survival, cysts appear craniad and caudad to the lesion and erode much of the scar together with viable neural tissue. Giving cytoxan or piromen did not result in any qualitative alteration of the scar matrix as evidenced by electron microscopy. Quantitative analysis revealed a slight reduction in the fibrous connective tissue component of the scar at 45--90 dpo, but this was transient when longer postoperative periods were studied. Trypsin caused a significant reduction in the amount of fibrous connective tissue with a concomitant increase in loose connective tissue and the appearance of a few distinctive, compact bundles of unmyelinated axons lacking Schwann cells. Consistent behavioral changes were not observed in any group which could distinguish them from the controls. Our results appear to contradict the findings of Matinian and Andreasian (1976) who reported return of normal sensori-motor function in 80% of their animals treated with topical and systemic trypsin. It is concluded that a major impediment to whatever longterm regenerative potential exists within the spinal cord is the lack of axonal guiding elements within the scar, but more importantly, the severe erosion of the remaining spinal cord due to cyst enlargement."}
{"id": "PMID:471189", "title": "Axon sprouting into segments of rat spinal cord adjacent to the site of a previous transection.", "content": "Transection of the spinal cord is adult Long-Evans Hooded rats is followed by the formation of a connective tissue matrix in the lesion site and the rapid erosion of the neural elements above and below this zone particularly within the dorsal white columns. In the period between 15--45 days after operation two significant events begin to occur. First the injured surfaces of the divided cord become invested by a glial limiting membrane (glia limitans) and, concomitantly, large numbers of axons ensheathed by Schwann cells sprout into the scar matrix and along the eroded dorsal column region. The injured surface of the spinal cord is highly irregular with deep, collagen-filled rifts into which the sprouting axons may probe and penetrate into the adjacent normal neuropil. Electron microscopic examination generally reveals the interposed glia limitans and that these fibres are usually restricted to the peripheral environment. However, as some axons approach the reconstituting glia limitans, they are enveloped by an astrocytic cytoplasmic process which may either displace the Schwann cell or encompass it together with the enclosed axons. This last phenomenon appears to precede the entry of some axons into the neuropil and suggests that the glia limitans may not necessarily represent an impenetrable barrier to the passage of regenerating axons into the CNS. Apparent maintenance of most of these fibres for periods of up to 3 months may suggest that viable, functional synapses were established upon available neuronal elements, but clear evidence of this could not be documented.", "contents": "Axon sprouting into segments of rat spinal cord adjacent to the site of a previous transection. Transection of the spinal cord is adult Long-Evans Hooded rats is followed by the formation of a connective tissue matrix in the lesion site and the rapid erosion of the neural elements above and below this zone particularly within the dorsal white columns. In the period between 15--45 days after operation two significant events begin to occur. First the injured surfaces of the divided cord become invested by a glial limiting membrane (glia limitans) and, concomitantly, large numbers of axons ensheathed by Schwann cells sprout into the scar matrix and along the eroded dorsal column region. The injured surface of the spinal cord is highly irregular with deep, collagen-filled rifts into which the sprouting axons may probe and penetrate into the adjacent normal neuropil. Electron microscopic examination generally reveals the interposed glia limitans and that these fibres are usually restricted to the peripheral environment. However, as some axons approach the reconstituting glia limitans, they are enveloped by an astrocytic cytoplasmic process which may either displace the Schwann cell or encompass it together with the enclosed axons. This last phenomenon appears to precede the entry of some axons into the neuropil and suggests that the glia limitans may not necessarily represent an impenetrable barrier to the passage of regenerating axons into the CNS. Apparent maintenance of most of these fibres for periods of up to 3 months may suggest that viable, functional synapses were established upon available neuronal elements, but clear evidence of this could not be documented."}
{"id": "PMID:471194", "title": "TSH secretion following acute heat exposure, in intact and in hypothalamic deafferentated male rats.", "content": "Male rats, intact (N) or with complete, anterior, or posterior hypothalamic deafferentations (CHD, AHD, or PHD, respectively), were acutely exposed to an environmental temperature of 36 degrees C, and serum TSH concentrations were determined by RIA. In intact animals, 30 min of exposure to elevated environmental temperature resulted in decreased serum TSH levels. In both CHD and AHD animals, basal serum TSH concentrations were reduced, and heat exposure produced further decreases. In the PHD group, no significant difference in resting serum TSH concentrations was observed, as compared to intacts. Exposure of these animals to 36 degrees C was followed by a reduction in this parameter, similar to that in intact rats. Exposure of intact animals to 3 nonthermal stress modalities produced no changes in serum TSH concentrations. These experiments demonstrate (1) that the hypothalamo-hypophyseal-thyroid axis responds specifically to heat exposure; (2) that extrahypothalamic centers which innervate the medial basal hypothalamus from the rostral direction maintain basal activity of the axis; and (3) that humoral factors, possibly extrahypothalamic thyrotrophin-releasing hormone carried to the median eminence via the CSF, may also mediate both basal and heat-induced secretory activity of the thyrotrophs.", "contents": "TSH secretion following acute heat exposure, in intact and in hypothalamic deafferentated male rats. Male rats, intact (N) or with complete, anterior, or posterior hypothalamic deafferentations (CHD, AHD, or PHD, respectively), were acutely exposed to an environmental temperature of 36 degrees C, and serum TSH concentrations were determined by RIA. In intact animals, 30 min of exposure to elevated environmental temperature resulted in decreased serum TSH levels. In both CHD and AHD animals, basal serum TSH concentrations were reduced, and heat exposure produced further decreases. In the PHD group, no significant difference in resting serum TSH concentrations was observed, as compared to intacts. Exposure of these animals to 36 degrees C was followed by a reduction in this parameter, similar to that in intact rats. Exposure of intact animals to 3 nonthermal stress modalities produced no changes in serum TSH concentrations. These experiments demonstrate (1) that the hypothalamo-hypophyseal-thyroid axis responds specifically to heat exposure; (2) that extrahypothalamic centers which innervate the medial basal hypothalamus from the rostral direction maintain basal activity of the axis; and (3) that humoral factors, possibly extrahypothalamic thyrotrophin-releasing hormone carried to the median eminence via the CSF, may also mediate both basal and heat-induced secretory activity of the thyrotrophs."}
{"id": "PMID:471195", "title": "Increased serum growth hormone and somatic growth in adult hamsters with hippocampal transections.", "content": "Somatic, endocrine, and behavioral correlates of growth and levels of voluntary running activity were measured in adult hamsters with hippocampal transections (HIPPO cuts) or in controls with transections of overlying cortex. Significant increase in serum concentration of growth hormone (GH) and decrease in pituitary concentration of GH were measured in HIPPO hamsters with a homologous radioimmunoassay method for hamster GH. HIPPO hamsters had increased: serum insulin concentration in fed state, food consumption, ponderal and linear growth, and percentage of body fat, and decreased levels of voluntary activity. Similarities between growth acceleration after HIPPO cuts and lesions of rostral medial septum suggest that fibers interconnecting, or passing through, the hippocampal formation and septum inhibit growth in adult hamsters.", "contents": "Increased serum growth hormone and somatic growth in adult hamsters with hippocampal transections. Somatic, endocrine, and behavioral correlates of growth and levels of voluntary running activity were measured in adult hamsters with hippocampal transections (HIPPO cuts) or in controls with transections of overlying cortex. Significant increase in serum concentration of growth hormone (GH) and decrease in pituitary concentration of GH were measured in HIPPO hamsters with a homologous radioimmunoassay method for hamster GH. HIPPO hamsters had increased: serum insulin concentration in fed state, food consumption, ponderal and linear growth, and percentage of body fat, and decreased levels of voluntary activity. Similarities between growth acceleration after HIPPO cuts and lesions of rostral medial septum suggest that fibers interconnecting, or passing through, the hippocampal formation and septum inhibit growth in adult hamsters."}
{"id": "PMID:471190", "title": "The cellular origin of lysosomal enzymes in the plaque in multiple sclerosis. II. A histochemical study with combined demonstration of myelin and acid phosphatase.", "content": "Existing techniques have been adapted and it has been possible to demonstrate acid phosphatase (APP) and myelin, lipid or astrocytic fibres in the same histological section. In normal controls APP was demonstrated in neurons, astrocytes, ependyma including choroid plexus epithelium and in pericytes, but not in oligodendrocytes. In multiple sclerosis (MS), APP positive cells were found in the plaque, plaque edge and macroscopically normal white matter. Contrary to some previous reports, not only macrophages, but also astrocytes in the MS plaque were found to be APP positive, although the distribution of enzyme in these cells was predominantly diffuse, unlike the particulate distribution in normal astrocytes and other APP positive cells: the significance of this finding is discussed. It is concluded that astrocytes and macrophages are the main source of the elevated levels of APP found biochemically in MS and that in old plaques astrocytes make the major contribution.", "contents": "The cellular origin of lysosomal enzymes in the plaque in multiple sclerosis. II. A histochemical study with combined demonstration of myelin and acid phosphatase. Existing techniques have been adapted and it has been possible to demonstrate acid phosphatase (APP) and myelin, lipid or astrocytic fibres in the same histological section. In normal controls APP was demonstrated in neurons, astrocytes, ependyma including choroid plexus epithelium and in pericytes, but not in oligodendrocytes. In multiple sclerosis (MS), APP positive cells were found in the plaque, plaque edge and macroscopically normal white matter. Contrary to some previous reports, not only macrophages, but also astrocytes in the MS plaque were found to be APP positive, although the distribution of enzyme in these cells was predominantly diffuse, unlike the particulate distribution in normal astrocytes and other APP positive cells: the significance of this finding is discussed. It is concluded that astrocytes and macrophages are the main source of the elevated levels of APP found biochemically in MS and that in old plaques astrocytes make the major contribution."}
{"id": "PMID:471196", "title": "DNA synthesis in the pituitary gland of the rat. Effect of sulpiride and postnatal maturation.", "content": "During development of the rat anterior pituitary gland (APG) there is a fall in DNA replication which is accompanied by a decline in the activity of the soluble DNA polymerase and of an endonuclease. This latter enzyme is capable of activating the DNA template for the DNA polymerase assay. Sulpiride sulfate, a drug known to produce prolactin release from the APG, increases thymidine incorporation in the APG 20 h after the injection. This drug also enhances the activity of the soluble DNA polymerase while that of the endonuclease and thymidine kinase does not change. The results suggest that the intracellular prolactin content regulates DNA replication in mammotrophs and that the soluble DNA polymerase plays an important role in this regulation.", "contents": "DNA synthesis in the pituitary gland of the rat. Effect of sulpiride and postnatal maturation. During development of the rat anterior pituitary gland (APG) there is a fall in DNA replication which is accompanied by a decline in the activity of the soluble DNA polymerase and of an endonuclease. This latter enzyme is capable of activating the DNA template for the DNA polymerase assay. Sulpiride sulfate, a drug known to produce prolactin release from the APG, increases thymidine incorporation in the APG 20 h after the injection. This drug also enhances the activity of the soluble DNA polymerase while that of the endonuclease and thymidine kinase does not change. The results suggest that the intracellular prolactin content regulates DNA replication in mammotrophs and that the soluble DNA polymerase plays an important role in this regulation."}
{"id": "PMID:471197", "title": "Specificity of short-loop feedback of luteinizing hormone in the rabbit.", "content": "The specificity of the gonadotropin short-loop feedback mechanism was investigated in castrated rabbits by blood sampling from an indwelling jugular venous catheter for follicle-stimulating hormone (FSH) after the intravenous administration of purified human luteinizing hormone (hLH). Although we have previously shown that such hLH administration results in a decrease of rabbit LH, no decrease in rabbit FSH was seen in these experiments. This specificity of LH feedback is compatible with the site of feedback being at the (1) pituitary level or (2) at the hypothalamic level provided two separate LH and FSH releasing factors exist.", "contents": "Specificity of short-loop feedback of luteinizing hormone in the rabbit. The specificity of the gonadotropin short-loop feedback mechanism was investigated in castrated rabbits by blood sampling from an indwelling jugular venous catheter for follicle-stimulating hormone (FSH) after the intravenous administration of purified human luteinizing hormone (hLH). Although we have previously shown that such hLH administration results in a decrease of rabbit LH, no decrease in rabbit FSH was seen in these experiments. This specificity of LH feedback is compatible with the site of feedback being at the (1) pituitary level or (2) at the hypothalamic level provided two separate LH and FSH releasing factors exist."}
{"id": "PMID:471192", "title": "Pseudo-intracellular collagen tangles in human spinal ganglia.", "content": "A new structure was found in the spinal ganglia of 6 out of 215 human autopsy cases with various disorders. It was composed of ballooned cells fully packed with fibrillar structures showing the same staining properties as collagen. Ultrastructurally the fibrillar structures were bundles of collagen fibres with a striation of about 70nm periodicity, and they were enclosed by the cell membrane invaginated in a very complex manner into the cytoplasm. This structure, which we named 'pseudo-intracellular collagen tangle', was considered to result from a disturbance in the releasing process of collagen fibre from fibroblasts. The aetiology of the structure is unknown, but it might represent one of regressive or senile changes in human spinal ganglia.", "contents": "Pseudo-intracellular collagen tangles in human spinal ganglia. A new structure was found in the spinal ganglia of 6 out of 215 human autopsy cases with various disorders. It was composed of ballooned cells fully packed with fibrillar structures showing the same staining properties as collagen. Ultrastructurally the fibrillar structures were bundles of collagen fibres with a striation of about 70nm periodicity, and they were enclosed by the cell membrane invaginated in a very complex manner into the cytoplasm. This structure, which we named 'pseudo-intracellular collagen tangle', was considered to result from a disturbance in the releasing process of collagen fibre from fibroblasts. The aetiology of the structure is unknown, but it might represent one of regressive or senile changes in human spinal ganglia."}
{"id": "PMID:471198", "title": "Regulation of the tuberoinfundibular and nigrostriatal systems. Evidence for different kinds of dopaminergic neurons in the brain.", "content": "The tuberoinfundibular dopaminergic (TIDA) neurons appear to present remarkable differences from the nigrostriatal and mesocortical dopaminergic (DA) systems. The hormonal feedback of prolactin on TIDA neurons differs from the neuronal feedback system operating in the striatum and olfactory tubercles. The uptake of dopamine appears to present the characteristics of a high affinity process in the terminals of the striatum and olfactory tubercle, whereas in the median eminence this process exhibits a much lower affinity. The pharmacological properties of postsynaptic receptors in the different DA pathways appear to be similar. However, the intracellular events which are ultimately manifested as physiological responses to dopamine may be completely different in the postsynaptic cells in the neostriatum and in the lactotrophs of the anterior pituitary. In addition, there is evidence suggesting that dopamine receptors in the anterior pituitary are more sensitive to the actions of agonists and antagonists than are dopamine receptors in the striatum.", "contents": "Regulation of the tuberoinfundibular and nigrostriatal systems. Evidence for different kinds of dopaminergic neurons in the brain. The tuberoinfundibular dopaminergic (TIDA) neurons appear to present remarkable differences from the nigrostriatal and mesocortical dopaminergic (DA) systems. The hormonal feedback of prolactin on TIDA neurons differs from the neuronal feedback system operating in the striatum and olfactory tubercles. The uptake of dopamine appears to present the characteristics of a high affinity process in the terminals of the striatum and olfactory tubercle, whereas in the median eminence this process exhibits a much lower affinity. The pharmacological properties of postsynaptic receptors in the different DA pathways appear to be similar. However, the intracellular events which are ultimately manifested as physiological responses to dopamine may be completely different in the postsynaptic cells in the neostriatum and in the lactotrophs of the anterior pituitary. In addition, there is evidence suggesting that dopamine receptors in the anterior pituitary are more sensitive to the actions of agonists and antagonists than are dopamine receptors in the striatum."}
{"id": "PMID:471193", "title": "Adult metachromatic leucodystrophy: clinicopathological report of two familial cases with slow course.", "content": "Two cases of adult metachromatic leucodystrophy in one family are reported. The main clinical features in both were predominantly psychiatric with alcoholism and an extremely long duration of the illness. Neuropathological examination revealed a similar distribution of the lesions in both, and scanty metachromatic accumulation in the CNS and not at all elsewhere. The great variability of the lengths of the courses is stressed. The duration in no way seems to be linked to the age of onset, except in the typical infantile form. The authors argue that different lengths of history correlate with distinct neuropathological findings, and may possibly be related to qualitative differences in the involved enzyme disturbance. Therefore, the authors suggest that the classification based on the age of onset be enlarged with a further one distinguishing between 'rapid' and 'slow' course types.", "contents": "Adult metachromatic leucodystrophy: clinicopathological report of two familial cases with slow course. Two cases of adult metachromatic leucodystrophy in one family are reported. The main clinical features in both were predominantly psychiatric with alcoholism and an extremely long duration of the illness. Neuropathological examination revealed a similar distribution of the lesions in both, and scanty metachromatic accumulation in the CNS and not at all elsewhere. The great variability of the lengths of the courses is stressed. The duration in no way seems to be linked to the age of onset, except in the typical infantile form. The authors argue that different lengths of history correlate with distinct neuropathological findings, and may possibly be related to qualitative differences in the involved enzyme disturbance. Therefore, the authors suggest that the classification based on the age of onset be enlarged with a further one distinguishing between 'rapid' and 'slow' course types."}
{"id": "PMID:471199", "title": "Anatomical study of a posterior cerebral lesion producing dyslexia.", "content": "After an \"occipital lobectomy\" that resulted in a severe dyslexia and a moderate dysgraphia-dyscalculia, anatomical study showed damage to the posterior extremity of the angular gyrus and degeneration in the posteroinferior pulvinar. This is in contrast to an earlier case that had degeneration in the anterosuperior pulvinor associated with a small anterior temporoparietal infarct and a well-documented receptive-expressive aphasia. However, the role of the pulvinar in speech function remains uncertain. The surgeon should be aware of the short distance between the angular gyrus and both the midline and the occipital pole because a lesion here during an \"occipital lobectomy\" produces a distressing and durable speech impairment.", "contents": "Anatomical study of a posterior cerebral lesion producing dyslexia. After an \"occipital lobectomy\" that resulted in a severe dyslexia and a moderate dysgraphia-dyscalculia, anatomical study showed damage to the posterior extremity of the angular gyrus and degeneration in the posteroinferior pulvinar. This is in contrast to an earlier case that had degeneration in the anterosuperior pulvinor associated with a small anterior temporoparietal infarct and a well-documented receptive-expressive aphasia. However, the role of the pulvinar in speech function remains uncertain. The surgeon should be aware of the short distance between the angular gyrus and both the midline and the occipital pole because a lesion here during an \"occipital lobectomy\" produces a distressing and durable speech impairment."}
{"id": "PMID:471200", "title": "Correlation of absorption coefficients with intracranial fluid protein concentrations and specific gravities.", "content": "Samples of ventricular cerebrospinal fluid, tumor cyst fluid, and subdural fluid were obtained from 30 patients at operation. The protein concentration and the specific gravity of each sample were measured and the corresponding mean absorption numbers were calculated from the numerical printout of the preoperative computerized tomogram. For fluids with a specific gravity greater than 1.005, a linear relationship was demonstrated between protein concentration and specific gravity. For protein concentrations greater than 300 mg/dl, there was a linear relationship between protein concentration and the mean absorption number. As the precision of present instrumentation improves, it is expected that a noninvasive technique for estimating intracranial protein concentration will have a number of clinical application.", "contents": "Correlation of absorption coefficients with intracranial fluid protein concentrations and specific gravities. Samples of ventricular cerebrospinal fluid, tumor cyst fluid, and subdural fluid were obtained from 30 patients at operation. The protein concentration and the specific gravity of each sample were measured and the corresponding mean absorption numbers were calculated from the numerical printout of the preoperative computerized tomogram. For fluids with a specific gravity greater than 1.005, a linear relationship was demonstrated between protein concentration and specific gravity. For protein concentrations greater than 300 mg/dl, there was a linear relationship between protein concentration and the mean absorption number. As the precision of present instrumentation improves, it is expected that a noninvasive technique for estimating intracranial protein concentration will have a number of clinical application."}
{"id": "PMID:471191", "title": "The early ultrastructural alterations in the rabbit cerebral and cerebellar cortex after compression ischaemia.", "content": "The ultrastructural alterations in the rabbit cerebral and cerebellar cortex resulting from 30 minutes complete, permanent cerebral ischaemia were studied. The ischaemia was induced by raising the intracranial pressure (ICP) above the systolic arterial pressure (compression ischaemia). Immediately after releasing the ICP the brain was fixed by intravascular glutaraldehyde perfusion. Samples from the cerebral and cerebellar cortex were processed for electron microscopy. The ultrastructural changes were relatively minor; there was a generalised, slight intracellular oedema, most prominent in the subpial area; the nuclear chromatin was clumped, the endoplasmic reticulum and cisternae of the golgi apparatus became somewhat dilated, the inner matrix of the slightly swollen mitochondria showed increased electron lucency, and microtubules and ribosomes began to loose their compact structure. These changes, unaccompanied by any extensive volumetric change of any cellular compartment, agree well with the recently presented hypothesis of two different types of anoxic-ischaemic nerve cell injury. This cellular reaction to complete, permanent compression ischaemia represents the type of injury that is seen resulting from ischaemic insults during which no flow of fluid irrigates the ischaemically injured cells.", "contents": "The early ultrastructural alterations in the rabbit cerebral and cerebellar cortex after compression ischaemia. The ultrastructural alterations in the rabbit cerebral and cerebellar cortex resulting from 30 minutes complete, permanent cerebral ischaemia were studied. The ischaemia was induced by raising the intracranial pressure (ICP) above the systolic arterial pressure (compression ischaemia). Immediately after releasing the ICP the brain was fixed by intravascular glutaraldehyde perfusion. Samples from the cerebral and cerebellar cortex were processed for electron microscopy. The ultrastructural changes were relatively minor; there was a generalised, slight intracellular oedema, most prominent in the subpial area; the nuclear chromatin was clumped, the endoplasmic reticulum and cisternae of the golgi apparatus became somewhat dilated, the inner matrix of the slightly swollen mitochondria showed increased electron lucency, and microtubules and ribosomes began to loose their compact structure. These changes, unaccompanied by any extensive volumetric change of any cellular compartment, agree well with the recently presented hypothesis of two different types of anoxic-ischaemic nerve cell injury. This cellular reaction to complete, permanent compression ischaemia represents the type of injury that is seen resulting from ischaemic insults during which no flow of fluid irrigates the ischaemically injured cells."}
{"id": "PMID:471201", "title": "Extracranial-intracranial arterial bypass in the treatment of aneurysms of the carotid and middle cerebral arteries.", "content": "Eleven patients with lesions of the internal carotid and middle cerebral arteries that could not be treated by direct approach are presented. Major vessel occlusion was chosen as definitive therapy. Prophylactic extracranial-intracranial bypass was employed in an attempt to reduce the incidence of ischemic complications. Emphasis is placed on complications, from which several important lessons were learned. Our best results occurred in patients with carotid artery lesions treated with bypass and immediate internal carotid ligation. We believe this to be an effective and safe mode of therapy in cases in which direct approach is not feasible. The low risk and high potential benefit of prophylactic bypass justifies its use before major vessel occlusion in selected cases.", "contents": "Extracranial-intracranial arterial bypass in the treatment of aneurysms of the carotid and middle cerebral arteries. Eleven patients with lesions of the internal carotid and middle cerebral arteries that could not be treated by direct approach are presented. Major vessel occlusion was chosen as definitive therapy. Prophylactic extracranial-intracranial bypass was employed in an attempt to reduce the incidence of ischemic complications. Emphasis is placed on complications, from which several important lessons were learned. Our best results occurred in patients with carotid artery lesions treated with bypass and immediate internal carotid ligation. We believe this to be an effective and safe mode of therapy in cases in which direct approach is not feasible. The low risk and high potential benefit of prophylactic bypass justifies its use before major vessel occlusion in selected cases."}
{"id": "PMID:471202", "title": "Traumatic lesions of the corpus callosum.", "content": "We investigated the mechanism of damage to the corpus callosum in a study of 46 brains obtained at the autopsies of patients with head injuries. After correlating the gross findings with the site of impact on the head, we think that such lesions in the corpus callosum are caused by stretching of callosal fibers and/or shearing forces in cases in which lateral movement or twisting of the brain occurs. This mechanism of the dynamic development of callosal injuries is also supported by the retraction bulb formation of axons found histologically in the corpus callosum.", "contents": "Traumatic lesions of the corpus callosum. We investigated the mechanism of damage to the corpus callosum in a study of 46 brains obtained at the autopsies of patients with head injuries. After correlating the gross findings with the site of impact on the head, we think that such lesions in the corpus callosum are caused by stretching of callosal fibers and/or shearing forces in cases in which lateral movement or twisting of the brain occurs. This mechanism of the dynamic development of callosal injuries is also supported by the retraction bulb formation of axons found histologically in the corpus callosum."}
{"id": "PMID:471203", "title": "Epidural measurement of intracranial pressure.", "content": "Although the measurement of intracranial pressure (ICP) is gaining widespread acceptance, the most desirable method of measurement is disputed. Subdural fluid-coupled techniques are associated with an increased risk of infection, and epidural techniques are associated with decreased accuracy. We investigated epidural measurement techniques and suggest that the necessary and sufficient criteria for accurate epidural measurement of ICP are adequate transducer size and stiffness, transducer-dura coplanarity, transducer-guard ring coplanarity, complete dural contact, and rigid fixation. An epidural transducer design was developed and prototypes were constructed using these principles. The transducer requires no percutaneous connections, fluid coupling, or batteries. Transducer accuracy was +/- 2.2 torr in bench stability studies lasting up to 198 days, +/- 3.0 torr in acute animal studies of less than 24 hours, and +/- 7.9 torr in chronic animal studies lasting up to 112 days. Error bounds are expressed such that 95% of individual measurements are expected to have error less than the bound; average error is one-third of the bound. Average transducer drift was 0.1 torr per day; our reported accuracy in chronic studies used drift correction from preimplantation data. We conclude that accurate measurement of ICP using an epidural transducer is feasible.", "contents": "Epidural measurement of intracranial pressure. Although the measurement of intracranial pressure (ICP) is gaining widespread acceptance, the most desirable method of measurement is disputed. Subdural fluid-coupled techniques are associated with an increased risk of infection, and epidural techniques are associated with decreased accuracy. We investigated epidural measurement techniques and suggest that the necessary and sufficient criteria for accurate epidural measurement of ICP are adequate transducer size and stiffness, transducer-dura coplanarity, transducer-guard ring coplanarity, complete dural contact, and rigid fixation. An epidural transducer design was developed and prototypes were constructed using these principles. The transducer requires no percutaneous connections, fluid coupling, or batteries. Transducer accuracy was +/- 2.2 torr in bench stability studies lasting up to 198 days, +/- 3.0 torr in acute animal studies of less than 24 hours, and +/- 7.9 torr in chronic animal studies lasting up to 112 days. Error bounds are expressed such that 95% of individual measurements are expected to have error less than the bound; average error is one-third of the bound. Average transducer drift was 0.1 torr per day; our reported accuracy in chronic studies used drift correction from preimplantation data. We conclude that accurate measurement of ICP using an epidural transducer is feasible."}
{"id": "PMID:471204", "title": "Sarcoma metastatic to the central nervous system: report of three cases and review of the literature.", "content": "Sarcoma metastatic to the central nervous system is generally considered to be rare. In this report three cases of sarcoma with metastasis to the central nervous system are presented. The case of rhabdomyosarcoma of the lung probably represents the first reported instance of such tumor metastatic to the central nervous system. The case of fibrosarcoma of the thigh is thought to be the first report of metastatic sarcoma with leptomeningeal dissemination. The metastatic brain lesions of the alveolar soft part sarcoma were incidental findings at autopsy. A summary of 22 cases previously reported and a brief discussion of the mode of metastasis of sarcoma, as compared to that of carcinoma, are also presented.", "contents": "Sarcoma metastatic to the central nervous system: report of three cases and review of the literature. Sarcoma metastatic to the central nervous system is generally considered to be rare. In this report three cases of sarcoma with metastasis to the central nervous system are presented. The case of rhabdomyosarcoma of the lung probably represents the first reported instance of such tumor metastatic to the central nervous system. The case of fibrosarcoma of the thigh is thought to be the first report of metastatic sarcoma with leptomeningeal dissemination. The metastatic brain lesions of the alveolar soft part sarcoma were incidental findings at autopsy. A summary of 22 cases previously reported and a brief discussion of the mode of metastasis of sarcoma, as compared to that of carcinoma, are also presented."}
{"id": "PMID:471205", "title": "Assessment of the postoperative course of excised brain abscess by computerized tomography.", "content": "We present two patients with brain abscess who underwent complete excision with a satisfactory clinical outcome although serial computerized tomography (CT) Scans in the early postoperative period seemed to indicate a large residual abscess in each case. We recommend treatment based on the clinical course rather than the CT scan appearance, indicate that earlier reports on this topic may be misleading, and suggest an explanation for these findings.", "contents": "Assessment of the postoperative course of excised brain abscess by computerized tomography. We present two patients with brain abscess who underwent complete excision with a satisfactory clinical outcome although serial computerized tomography (CT) Scans in the early postoperative period seemed to indicate a large residual abscess in each case. We recommend treatment based on the clinical course rather than the CT scan appearance, indicate that earlier reports on this topic may be misleading, and suggest an explanation for these findings."}
{"id": "PMID:471206", "title": "Symptomatic cyst of the choroid plexus of the lateral ventricle.", "content": "A case of symptomatic cyst of the choroid plexus of a lateral ventricle in a child is reported. The characteristics of the case are compared with those of the other three cases in the literature.", "contents": "Symptomatic cyst of the choroid plexus of the lateral ventricle. A case of symptomatic cyst of the choroid plexus of a lateral ventricle in a child is reported. The characteristics of the case are compared with those of the other three cases in the literature."}
{"id": "PMID:471207", "title": "Cervical dermal sinus and intramedullary spinal cord abscess: case report.", "content": "Congenital dermal sinuses in the cervical region are rare. A case report is presented in which the persistent anomaly led to formation of an intramedullary spinal cord abscess. The clinical presentation, radiological diagnosis, and surgical management are discussed.", "contents": "Cervical dermal sinus and intramedullary spinal cord abscess: case report. Congenital dermal sinuses in the cervical region are rare. A case report is presented in which the persistent anomaly led to formation of an intramedullary spinal cord abscess. The clinical presentation, radiological diagnosis, and surgical management are discussed."}
{"id": "PMID:471212", "title": "The normal angiographic sylvian point on the lateral cerebral angiogram.", "content": "The angiographic sylvian point (ASP) is one of the most useful landmarks on cerebral angiograms for detecting retrosylvian masses. Although it is suggested to be the halfway point on the clinoparietal line (CPL), its exact normal position has not been defined. The lateral carotid angiograms of 100 consecutive patients from 22 to 65 years of age were used to study the normal ASP in relation to the CPL. Patients with severe neurological deficits or angiographic evidence of hydrocephalus, severe vascular disease or mass lesions were eliminated from this study. In our 100 normals, the normal ASP was within 8 mm above and below the CPL, and in the majority (82%) it was located behind the midpoint of the CPL. It was not situated more than 14.4 mm posterior and never more than 3.1 mm anterior to the midpoint. Application of these normal relationships facilitates detection of small, deep retrosylvian masses occupying the medial portions of the parietal, occipital and temporal lobes.", "contents": "The normal angiographic sylvian point on the lateral cerebral angiogram. The angiographic sylvian point (ASP) is one of the most useful landmarks on cerebral angiograms for detecting retrosylvian masses. Although it is suggested to be the halfway point on the clinoparietal line (CPL), its exact normal position has not been defined. The lateral carotid angiograms of 100 consecutive patients from 22 to 65 years of age were used to study the normal ASP in relation to the CPL. Patients with severe neurological deficits or angiographic evidence of hydrocephalus, severe vascular disease or mass lesions were eliminated from this study. In our 100 normals, the normal ASP was within 8 mm above and below the CPL, and in the majority (82%) it was located behind the midpoint of the CPL. It was not situated more than 14.4 mm posterior and never more than 3.1 mm anterior to the midpoint. Application of these normal relationships facilitates detection of small, deep retrosylvian masses occupying the medial portions of the parietal, occipital and temporal lobes."}
{"id": "PMID:471213", "title": "Persistent proatlantal intersegmental artery and occipital artery originating from internal carotid artery.", "content": "A case of a combined anomaly of persistent hypoglossal and proatlantal intersegmental arteries, proved on arteriogram obtained by the insertion of a needle into the common trunk of both arteries, is presented. In addition, a case of anomalous occipital artery arising from the internal carotid artery associated with an intracranial aneurysm and arteriovenous malformation is presented. Based upon analysis of the persistent proatlantal intersegmental artery and the anomalous occipital artery, we conclude that both arteries have an identical embryological origin.", "contents": "Persistent proatlantal intersegmental artery and occipital artery originating from internal carotid artery. A case of a combined anomaly of persistent hypoglossal and proatlantal intersegmental arteries, proved on arteriogram obtained by the insertion of a needle into the common trunk of both arteries, is presented. In addition, a case of anomalous occipital artery arising from the internal carotid artery associated with an intracranial aneurysm and arteriovenous malformation is presented. Based upon analysis of the persistent proatlantal intersegmental artery and the anomalous occipital artery, we conclude that both arteries have an identical embryological origin."}
{"id": "PMID:471214", "title": "Computed tomography and intracranial aspergillosis.", "content": "A case of aspergillosis of the central nervous system with multiple granulomas examined by computed tomography is reported. The findings are those of chronic mass lesions with and without calcium deposits. An associated cystic configuration probably is not specific for aspergillomas.", "contents": "Computed tomography and intracranial aspergillosis. A case of aspergillosis of the central nervous system with multiple granulomas examined by computed tomography is reported. The findings are those of chronic mass lesions with and without calcium deposits. An associated cystic configuration probably is not specific for aspergillomas."}
{"id": "PMID:471215", "title": "Pseudoaneurysm formation secondary to spontaneous thrombosis of a massive cerebral arteriovenous malformation.", "content": "A case of a large cerebral arteriovenous malformation (AVM) with spontaneous partial thrombosis and intracerebral hematoma is reported. The unusual and unique features of the radionuclide brain scan and cerebral angiogram are described. The characteristic angiographic features of residual tortuosity and dilatation or pseudoaneurysm formation at the bifurcations of the feeding arteries in a partially thrombosed cerebral AVM have not been described previously in the literature.", "contents": "Pseudoaneurysm formation secondary to spontaneous thrombosis of a massive cerebral arteriovenous malformation. A case of a large cerebral arteriovenous malformation (AVM) with spontaneous partial thrombosis and intracerebral hematoma is reported. The unusual and unique features of the radionuclide brain scan and cerebral angiogram are described. The characteristic angiographic features of residual tortuosity and dilatation or pseudoaneurysm formation at the bifurcations of the feeding arteries in a partially thrombosed cerebral AVM have not been described previously in the literature."}
{"id": "PMID:471216", "title": "Congenital absence of a pedicle in the cervical spine: a new approach with CT scan.", "content": "After complete review of the literature concerning the congenital absent pedicle syndrome, another typical case is presented. In the opinion of the authors, the diagnostic importance of the associated clinical symptoms is doubtful. Furthermore they demonstrate the great value of CT in the differential diagnosis of this lesion.", "contents": "Congenital absence of a pedicle in the cervical spine: a new approach with CT scan. After complete review of the literature concerning the congenital absent pedicle syndrome, another typical case is presented. In the opinion of the authors, the diagnostic importance of the associated clinical symptoms is doubtful. Furthermore they demonstrate the great value of CT in the differential diagnosis of this lesion."}
{"id": "PMID:471217", "title": "Factors influencing the development of periventricular lucencies in patients with raised intracranial pressure.", "content": "Sixty patients with raised intracranial pressure and lowered attenuation areas around the lateral ventricles ('periventricular lucency', PVL) on CT scanning were reviewed, and compared with a control group of 90 similar patients who did not have PVL. It was confirmed that PVL tends to occur in patients with acute or subacute obstructive hydrocephalus due to a tumour, and is more common in the presence of papilloedema and/or a decreased level of consciousness. Patients with very dilated lateral ventricles did not in general have PVL, but it was frequently seen in association with diastasis of the sutures. Twelve patients with PVL had no other clinical or radiological indication of raised intracranial pressure. Comparison with the control cases did not reveal any reason as to why some patients should develop PVL while others did not.", "contents": "Factors influencing the development of periventricular lucencies in patients with raised intracranial pressure. Sixty patients with raised intracranial pressure and lowered attenuation areas around the lateral ventricles ('periventricular lucency', PVL) on CT scanning were reviewed, and compared with a control group of 90 similar patients who did not have PVL. It was confirmed that PVL tends to occur in patients with acute or subacute obstructive hydrocephalus due to a tumour, and is more common in the presence of papilloedema and/or a decreased level of consciousness. Patients with very dilated lateral ventricles did not in general have PVL, but it was frequently seen in association with diastasis of the sutures. Twelve patients with PVL had no other clinical or radiological indication of raised intracranial pressure. Comparison with the control cases did not reveal any reason as to why some patients should develop PVL while others did not."}
{"id": "PMID:471218", "title": "Computed tomography and spinal dysraphism: clinical and phantom studies.", "content": "Two cases illustrating the value of CT in the assessment of spinal dysraphic tissue are presented. In one case, the configuration and origins of two osseous diastematomyelic spurs were shown well; in the second case, the CT recognition of a sacral lipoma led to air myelographic confirmation of the tumor and tethered cord. CT phantom studies indicated that dysraphic tissues, such as fat, cartilage, and fibrous tissue, are better identified and quantitated in the spinal canal when surrounded by air. Varying degrees of image degradation occur with water (simulating CSF) or metrizamide.", "contents": "Computed tomography and spinal dysraphism: clinical and phantom studies. Two cases illustrating the value of CT in the assessment of spinal dysraphic tissue are presented. In one case, the configuration and origins of two osseous diastematomyelic spurs were shown well; in the second case, the CT recognition of a sacral lipoma led to air myelographic confirmation of the tumor and tethered cord. CT phantom studies indicated that dysraphic tissues, such as fat, cartilage, and fibrous tissue, are better identified and quantitated in the spinal canal when surrounded by air. Varying degrees of image degradation occur with water (simulating CSF) or metrizamide."}
{"id": "PMID:471219", "title": "Metrizamide cisternography and computed tomography for the investigation of pituitary lesions.", "content": "Metrizamide cisternography and computed tomography (CT) have been performed on 22 patients suspected of having pituitary or suprasellar lesions, using a low dose of metrizamide. These patients would otherwise have been subjected to air encephalography. The technique is described and the results are presented. The lesions demonstrated included empty sellae and suprasellar extensions of pituitary tumours. We also identified those patients in whom there was no suprasellar extension of a pituitary  tumour. The diagnostic information required was obtained in all cases by a combination of the two techniques and air encephalography was avoided in all but one case. The side effects were mild and no serious complications were encountered. We consider this combination of methods to be very useful for the investigation of pituitary lesions.", "contents": "Metrizamide cisternography and computed tomography for the investigation of pituitary lesions. Metrizamide cisternography and computed tomography (CT) have been performed on 22 patients suspected of having pituitary or suprasellar lesions, using a low dose of metrizamide. These patients would otherwise have been subjected to air encephalography. The technique is described and the results are presented. The lesions demonstrated included empty sellae and suprasellar extensions of pituitary tumours. We also identified those patients in whom there was no suprasellar extension of a pituitary  tumour. The diagnostic information required was obtained in all cases by a combination of the two techniques and air encephalography was avoided in all but one case. The side effects were mild and no serious complications were encountered. We consider this combination of methods to be very useful for the investigation of pituitary lesions."}
{"id": "PMID:471220", "title": "Angiotomography for aneurysms and arteriovenous malformations of the head and neck.", "content": "Angiotomographic studies were performed preoperatively on 201 arterial aneurysms and arteriovenous malformations (AVMs) of the head and neck. There were two extracranial aneurysms, 186 intracranial aneurysms and 13 intracranial AVMs. The equipment used was the Philips NeuroDiagnost, either armed with a single multisection cassett for single-phase angiotomography or a triple cassette system on a motor-driven prism for serial amgiotomography. Angiotomography was limited to lesions where its use promised to add significantly to the information already obtained from conventional angiograms. Angiotomography was found to be of particular value for the differentiation of small aneurysms from normal vascular structures, for the study of morphology and topography of more or less spherical aneurysms in close proximity to a number of neighboring arteries, for the study of giant aneurysms, for the demonstration of very small AVMs (microangiomas) and for the evaluation of feeding and draining vessels of midline AVMs.", "contents": "Angiotomography for aneurysms and arteriovenous malformations of the head and neck. Angiotomographic studies were performed preoperatively on 201 arterial aneurysms and arteriovenous malformations (AVMs) of the head and neck. There were two extracranial aneurysms, 186 intracranial aneurysms and 13 intracranial AVMs. The equipment used was the Philips NeuroDiagnost, either armed with a single multisection cassett for single-phase angiotomography or a triple cassette system on a motor-driven prism for serial amgiotomography. Angiotomography was limited to lesions where its use promised to add significantly to the information already obtained from conventional angiograms. Angiotomography was found to be of particular value for the differentiation of small aneurysms from normal vascular structures, for the study of morphology and topography of more or less spherical aneurysms in close proximity to a number of neighboring arteries, for the study of giant aneurysms, for the demonstration of very small AVMs (microangiomas) and for the evaluation of feeding and draining vessels of midline AVMs."}
{"id": "PMID:471221", "title": "Cerebral arterial air embolism due to an esophago-atrial fistula seen on CT.", "content": "A case of cerebral air embolism from a rather unusual cause is reported; an esophago-cardiac fistula permitted food particles and air to enter the systemic arterial circulation. Massive embolization caused the patient to become deeply comatose rather suddenly. The computed tomogram (CT) revealed massive cerebral edema with the contradictory finding of wide superficial subarachnoid spaces. These subarachnoid spaces on further evaluation proved to be air in the cerebral vessels.", "contents": "Cerebral arterial air embolism due to an esophago-atrial fistula seen on CT. A case of cerebral air embolism from a rather unusual cause is reported; an esophago-cardiac fistula permitted food particles and air to enter the systemic arterial circulation. Massive embolization caused the patient to become deeply comatose rather suddenly. The computed tomogram (CT) revealed massive cerebral edema with the contradictory finding of wide superficial subarachnoid spaces. These subarachnoid spaces on further evaluation proved to be air in the cerebral vessels."}
{"id": "PMID:471222", "title": "Morphology of the cervical spinal cord on computed myelography.", "content": "To ensure adequate use of the technique of computed myelography (CM) it is necessary to have an exact picture of the morphology of the normal spinal cord as demonstrated by this technique. This has been obtained by studying the morphology and measuring the frontal and sagittal diameter of the cervical cord in 20 patients. The normal values are presented. The changes of this morphology in one patient with a tumour, one patient with atrophy of the spinal cord and in some patients with congenital malformations are also reported.", "contents": "Morphology of the cervical spinal cord on computed myelography. To ensure adequate use of the technique of computed myelography (CM) it is necessary to have an exact picture of the morphology of the normal spinal cord as demonstrated by this technique. This has been obtained by studying the morphology and measuring the frontal and sagittal diameter of the cervical cord in 20 patients. The normal values are presented. The changes of this morphology in one patient with a tumour, one patient with atrophy of the spinal cord and in some patients with congenital malformations are also reported."}
{"id": "PMID:471223", "title": "Xenon enhancement for computed tomography of the spinal cord.", "content": "The spinal cord is not consistently shown by plain computed tomography or with iodide enhancement. Xenon enhancement increases the attenuation of the spinal cord and visualization is improved considerably; fluid-filled cavities, including syringohydromyelia, do not enhance and are clearly defined.", "contents": "Xenon enhancement for computed tomography of the spinal cord. The spinal cord is not consistently shown by plain computed tomography or with iodide enhancement. Xenon enhancement increases the attenuation of the spinal cord and visualization is improved considerably; fluid-filled cavities, including syringohydromyelia, do not enhance and are clearly defined."}
{"id": "PMID:471225", "title": "The projection of the calcified pineal gland on slightly rotated AP films as a reliable landmark. A CT study.", "content": "The projection of the pineal gland between the two outer tables of the skull in the AP film has been reconstructed on the basis of the CT print of the head, with and without lateral rotation. In this experimental approach it is proven that rotation up to 5 degrees does not influence the position of the pineal gland on the AP film. The range of the normal position of the pineal in the frontal plane is defined and the normal distribution is given.", "contents": "The projection of the calcified pineal gland on slightly rotated AP films as a reliable landmark. A CT study. The projection of the pineal gland between the two outer tables of the skull in the AP film has been reconstructed on the basis of the CT print of the head, with and without lateral rotation. In this experimental approach it is proven that rotation up to 5 degrees does not influence the position of the pineal gland on the AP film. The range of the normal position of the pineal in the frontal plane is defined and the normal distribution is given."}
{"id": "PMID:471226", "title": "Computed tomography in alcoholic cerebellar atrophy.", "content": "This is a controlled CT evaluation of the infratentorial region in 41 male alcoholics under age 35. Criteria for the presence of atrophy are outlined. Twelve patients had cerebellar atrophy. Vermian atrophy was present in all. Atrophy of the cerebellar hemispheres was demonstrated in eight patients as well. The results are statistically significant when compared to an age-matched group of 40 non-alcoholic males among whom two cases of vermian atrophy were found. There were clinical signs of alcoholic cerebellar atrophy in one patient only. The disparity between the clinical and the radiological data are discussed with reference to previous pneumoencephalographic findings.", "contents": "Computed tomography in alcoholic cerebellar atrophy. This is a controlled CT evaluation of the infratentorial region in 41 male alcoholics under age 35. Criteria for the presence of atrophy are outlined. Twelve patients had cerebellar atrophy. Vermian atrophy was present in all. Atrophy of the cerebellar hemispheres was demonstrated in eight patients as well. The results are statistically significant when compared to an age-matched group of 40 non-alcoholic males among whom two cases of vermian atrophy were found. There were clinical signs of alcoholic cerebellar atrophy in one patient only. The disparity between the clinical and the radiological data are discussed with reference to previous pneumoencephalographic findings."}
{"id": "PMID:471227", "title": "The Evans ratio for ventricular size: a calculation error.", "content": "The suggestion that the Evans ratio, the ratio of the transverse diameter of the anterior horns of the lateral ventricles to the internal diameter of the skull, was a more significant quantity than the ventricular measurement alone was based on a calculation error. The value of this ratio and other such ratios involving linear skull measurements is unproven.", "contents": "The Evans ratio for ventricular size: a calculation error. The suggestion that the Evans ratio, the ratio of the transverse diameter of the anterior horns of the lateral ventricles to the internal diameter of the skull, was a more significant quantity than the ventricular measurement alone was based on a calculation error. The value of this ratio and other such ratios involving linear skull measurements is unproven."}
{"id": "PMID:471228", "title": "Ectasia of the basilar artery: the breadth of the clinical spectrum and the diagnostic value of computed tomography.", "content": "Five angiographically confirmed cases of ectasia of the basilar artery are described. Three patients presented with the classical combination of lower cranial nerve, bulbar, cerebellar and long tract signs and dementia, one as obstructive hydrocephalus with papilloedema, and the fifth with symptoms of chiasmal compression. All were above middle age and hypertensive. CT scanning demonstrated a partially calcified, tubular, enhancing prepontine and suprasellar mass in every case. Ventricular enlargement and cerebral parenchymal abnormalities were also present. These appearances are sufficiently characteristic to make invasive radiological investigation unnecessary.", "contents": "Ectasia of the basilar artery: the breadth of the clinical spectrum and the diagnostic value of computed tomography. Five angiographically confirmed cases of ectasia of the basilar artery are described. Three patients presented with the classical combination of lower cranial nerve, bulbar, cerebellar and long tract signs and dementia, one as obstructive hydrocephalus with papilloedema, and the fifth with symptoms of chiasmal compression. All were above middle age and hypertensive. CT scanning demonstrated a partially calcified, tubular, enhancing prepontine and suprasellar mass in every case. Ventricular enlargement and cerebral parenchymal abnormalities were also present. These appearances are sufficiently characteristic to make invasive radiological investigation unnecessary."}
{"id": "PMID:471229", "title": "Giant aneurysms of the posterior fossa suspected as neoplasms on computed tomography.", "content": "Giant intracranial aneurysms of the vertebral and basilar artery can be mistakenly diagnosed as neoplasms of the posterior fossa on the basis of their clinical presentation and CT findings. The following report of three cases of tumorous aneurysms emphasizes the complementary value of angiography and computed tomography for the localization and correct diagnosis of space-occupying lesions of the posterior fossa.", "contents": "Giant aneurysms of the posterior fossa suspected as neoplasms on computed tomography. Giant intracranial aneurysms of the vertebral and basilar artery can be mistakenly diagnosed as neoplasms of the posterior fossa on the basis of their clinical presentation and CT findings. The following report of three cases of tumorous aneurysms emphasizes the complementary value of angiography and computed tomography for the localization and correct diagnosis of space-occupying lesions of the posterior fossa."}
{"id": "PMID:471230", "title": "Intracranial fibromuscular dysplasia and stroke in children.", "content": "Fibromuscular dysplasia (FMD) is an arterial lesion of unknown origin which may sometimes affect intracranial arteries. In recent years a few rare cases, mostly involving younger people, have been described. The present case concerns a child of 8 years in whom the finding of hemiplegia led to a diagnosis of intracranial fibrodysplasia which was proven by arteriography and biopsy. This arterial lesion is described within the context of stroke in children.", "contents": "Intracranial fibromuscular dysplasia and stroke in children. Fibromuscular dysplasia (FMD) is an arterial lesion of unknown origin which may sometimes affect intracranial arteries. In recent years a few rare cases, mostly involving younger people, have been described. The present case concerns a child of 8 years in whom the finding of hemiplegia led to a diagnosis of intracranial fibrodysplasia which was proven by arteriography and biopsy. This arterial lesion is described within the context of stroke in children."}
{"id": "PMID:471277", "title": "[Treatment of acute pancreatitis with glucagon].", "content": "Personal experience in the treatment of acute pancreatitis with glucagon is reported. Results are highly satisfactory and encouraging in oedematous, interstitial and circumscribed steatonecrotic forms, disappointing where the disease has caused extensive damage to the gland.", "contents": "[Treatment of acute pancreatitis with glucagon]. Personal experience in the treatment of acute pancreatitis with glucagon is reported. Results are highly satisfactory and encouraging in oedematous, interstitial and circumscribed steatonecrotic forms, disappointing where the disease has caused extensive damage to the gland."}
{"id": "PMID:471278", "title": "[Calcified pseudocyst of the spleen. Report of a case].", "content": "The description of a case of calcific pseudocyst of the spleen has provided the opportunity for a review of the literature on the subject and for further study, on the basis of clinical and anatomo-pathological data, of the problem of the aetiopathogenesis of non-parasite splenic cysts.", "contents": "[Calcified pseudocyst of the spleen. Report of a case]. The description of a case of calcific pseudocyst of the spleen has provided the opportunity for a review of the literature on the subject and for further study, on the basis of clinical and anatomo-pathological data, of the problem of the aetiopathogenesis of non-parasite splenic cysts."}
{"id": "PMID:471279", "title": "[Spontaneous rupture of the subdiaphragmatic esophagus].", "content": "Personal experience with an extremely rare clinical picture, spontaneous rupture of the subdiaphragmatic oesophagus is reported. In the case in question, the laceration occurred in a free peritoneum,  unlike what occurred in the first reported case, that of Strauch and Lynch in 1965, where the lesion was retroperitoneal. On the basis of this experience, certain pathogenetic and diagnostic factors are discussed, but most attention is paid to the surgical treatment of this exceptional lesion.", "contents": "[Spontaneous rupture of the subdiaphragmatic esophagus]. Personal experience with an extremely rare clinical picture, spontaneous rupture of the subdiaphragmatic oesophagus is reported. In the case in question, the laceration occurred in a free peritoneum,  unlike what occurred in the first reported case, that of Strauch and Lynch in 1965, where the lesion was retroperitoneal. On the basis of this experience, certain pathogenetic and diagnostic factors are discussed, but most attention is paid to the surgical treatment of this exceptional lesion."}
{"id": "PMID:471280", "title": "[Segmentary necrosis of the main bile pathway. Presentation of a clinical case].", "content": "Reference is made to a personal case in an examination of the more controversial aspects of segmentary necrosis of the main bile pathway. Attention is drawn to the leading features of the pathogenetic evolution of this lesion. Bearing in mind the almost insurmountable difficulty of formulating a preoperative diagnosis, an examination is made of the possible therapeutic solutions. In the light of the data presented, the suggestion is made that the only effective prevention is offered by early surgical management of all pathological situations affecting the main bile pathway that may, if neglected, lead to the setting up of segmentary necrosis within it.", "contents": "[Segmentary necrosis of the main bile pathway. Presentation of a clinical case]. Reference is made to a personal case in an examination of the more controversial aspects of segmentary necrosis of the main bile pathway. Attention is drawn to the leading features of the pathogenetic evolution of this lesion. Bearing in mind the almost insurmountable difficulty of formulating a preoperative diagnosis, an examination is made of the possible therapeutic solutions. In the light of the data presented, the suggestion is made that the only effective prevention is offered by early surgical management of all pathological situations affecting the main bile pathway that may, if neglected, lead to the setting up of segmentary necrosis within it."}
{"id": "PMID:471282", "title": "[\"Cold\" nodules of the thyroid gland. (Clinical statistical study)].", "content": "A series of 718 scintigraphically cold thyroid nodules observed between 1965 and 1976 at the Turin University Endocrine Surgery Centre is presented. This form was more common in women (80%) and mainly occurred in the 3rd and 5th decade of life. Malignancy was present in 88 cases (12.27%): 49 papilliferous tumours, 25 follicular, 9 Hurthle-cell, and 5 anaplastic. A reference is expressed for unilateral lobectomy followed by suppressive management as opposed to total thyroidectomy since malignancy is low in forms where a tumour has a single site in a cold nodule. Furthermore, this approach spares the patient from the severe consequences of inevitable post-operative hypothyroidism. Total resection is, however, mandatory in anaplastic forms. A detailed account is offered of the typical echotomograms associated with individual pathological situations. These are enough to support a preoperative diagnosis of malignancy.", "contents": "[\"Cold\" nodules of the thyroid gland. (Clinical statistical study)]. A series of 718 scintigraphically cold thyroid nodules observed between 1965 and 1976 at the Turin University Endocrine Surgery Centre is presented. This form was more common in women (80%) and mainly occurred in the 3rd and 5th decade of life. Malignancy was present in 88 cases (12.27%): 49 papilliferous tumours, 25 follicular, 9 Hurthle-cell, and 5 anaplastic. A reference is expressed for unilateral lobectomy followed by suppressive management as opposed to total thyroidectomy since malignancy is low in forms where a tumour has a single site in a cold nodule. Furthermore, this approach spares the patient from the severe consequences of inevitable post-operative hypothyroidism. Total resection is, however, mandatory in anaplastic forms. A detailed account is offered of the typical echotomograms associated with individual pathological situations. These are enough to support a preoperative diagnosis of malignancy."}
{"id": "PMID:471283", "title": "[Carcinoma of the convergence of the hepatic ducts. A case treated with extended hepatic resection].", "content": "The case of a patient with carcinoma of the convergence of the hepatic ducts successfully treated with more extensive hepatic resection is reported. Stress is laid on the problems of exact preoperative diagnosis and of treatment. Most operations are in fact purely palliative and the literature contains very few cases of hepatic resection for neoplasia of the convergence.", "contents": "[Carcinoma of the convergence of the hepatic ducts. A case treated with extended hepatic resection]. The case of a patient with carcinoma of the convergence of the hepatic ducts successfully treated with more extensive hepatic resection is reported. Stress is laid on the problems of exact preoperative diagnosis and of treatment. Most operations are in fact purely palliative and the literature contains very few cases of hepatic resection for neoplasia of the convergence."}
{"id": "PMID:471284", "title": "[Metabolic changes after extended hepatic resection].", "content": "Two cases of right hepatic lobe resection, one due to trauma and the other to echinococcosis. Adequate support therapy can be used to control the protein, lipid and sugar metabolic changes that occur after this operation. The seriousness of the postoperative course is related to the amount of healthy tissue removed.", "contents": "[Metabolic changes after extended hepatic resection]. Two cases of right hepatic lobe resection, one due to trauma and the other to echinococcosis. Adequate support therapy can be used to control the protein, lipid and sugar metabolic changes that occur after this operation. The seriousness of the postoperative course is related to the amount of healthy tissue removed."}
{"id": "PMID:471285", "title": "[Endoscopic papillotomy. Indications and limitations].", "content": "Personal experience of 161 cases of endoscopic papillotomy (EP) is reported. Indications and contra-indications are considered in relation to the therapeutic effectiveness of the technique and to the lower percentage of morbidity and mortality connected to it with respect to that following on laparatomic surgery. On the basis of results obtained and of the data reported in the literature, it is pointed out that EP represents real progress in the treatment of calculosis of the hepatocholedochus and of stenosing papillitis.", "contents": "[Endoscopic papillotomy. Indications and limitations]. Personal experience of 161 cases of endoscopic papillotomy (EP) is reported. Indications and contra-indications are considered in relation to the therapeutic effectiveness of the technique and to the lower percentage of morbidity and mortality connected to it with respect to that following on laparatomic surgery. On the basis of results obtained and of the data reported in the literature, it is pointed out that EP represents real progress in the treatment of calculosis of the hepatocholedochus and of stenosing papillitis."}
{"id": "PMID:471286", "title": "[Transgastric lymphadenography].", "content": "The transgastric lymphadenography technique carried out by the endoscopic route in 52 patients at the Digestive Endoscopy Centre of the 3rd Surgical Clinic of Rome University is described. On the basis of personal results it is considered that transgastric lymphadenography represents an interesting technique for the study of perigastric lymphnodes in normal and pathologic conditions. It is thought that it could also be useful in the study of the abdominal lymphatics although further verification and a larger series are necessary.", "contents": "[Transgastric lymphadenography]. The transgastric lymphadenography technique carried out by the endoscopic route in 52 patients at the Digestive Endoscopy Centre of the 3rd Surgical Clinic of Rome University is described. On the basis of personal results it is considered that transgastric lymphadenography represents an interesting technique for the study of perigastric lymphnodes in normal and pathologic conditions. It is thought that it could also be useful in the study of the abdominal lymphatics although further verification and a larger series are necessary."}
{"id": "PMID:471287", "title": "[Carcinoma with a branchial origin].", "content": "Carcinoma was observed in a patient with a branchial cyst of about 40 years' standing. The neoformation was removed, but the patient died as a result of cardiocirculatory complications a little more than a year later. Note is taken of the rarity of this form and the compliance of the reported case with the criteria laid down by Martin et al. in 1950.", "contents": "[Carcinoma with a branchial origin]. Carcinoma was observed in a patient with a branchial cyst of about 40 years' standing. The neoformation was removed, but the patient died as a result of cardiocirculatory complications a little more than a year later. Note is taken of the rarity of this form and the compliance of the reported case with the criteria laid down by Martin et al. in 1950."}
{"id": "PMID:471288", "title": "[Hepatic hamartoma. Description of a case in a child].", "content": "A case of hepatic hamartoma in a child of 13 months, treated with atypical resection of the right lobe of the liver, is reported. The tumour was of whitish colour and weighed 800 g. Its size was 16 X 9 X 10 cm and its section revealed a hyaline-fibrous appearance with reddish areas and others of cystic appearance. At follow-up, the child did not complain of digestive trouble and grew normally.", "contents": "[Hepatic hamartoma. Description of a case in a child]. A case of hepatic hamartoma in a child of 13 months, treated with atypical resection of the right lobe of the liver, is reported. The tumour was of whitish colour and weighed 800 g. Its size was 16 X 9 X 10 cm and its section revealed a hyaline-fibrous appearance with reddish areas and others of cystic appearance. At follow-up, the child did not complain of digestive trouble and grew normally."}
{"id": "PMID:471289", "title": "[A case of retroperitoneal cystic lymphangioma].", "content": "A case of retroperitoneal cystic lymphangioma in a 62-yr-old woman presented as symptom-free abdominal swelling. The intraoperative diagnosis was confirmed histologically. Radical management consisted of stripping of the cyst from its adjacent structures. The postoperative course was uneventful.", "contents": "[A case of retroperitoneal cystic lymphangioma]. A case of retroperitoneal cystic lymphangioma in a 62-yr-old woman presented as symptom-free abdominal swelling. The intraoperative diagnosis was confirmed histologically. Radical management consisted of stripping of the cyst from its adjacent structures. The postoperative course was uneventful."}
{"id": "PMID:471290", "title": "[Late obstructions of aorto-bifemoral prostheses].", "content": "The factors underlying late thrombosis of aortobifemoral by-pass are analysed on the basis of personal experience and reported data. They are considered to be due to progressive degradation of the prosthesic tissue and, particularly, to the lack of an adequate back flow route, apart from any faults in the actual construction of the femoral anastomosis. Treatment of the thrombotic complication involves considerable technical and operating problems which have two main solutions: substitution of the thrombosed branch, or its disobstruction. Intimately linked with restoral of by-pass patency is the reconstruction of an adequate back flow route either by means of profundaplasty or by extending the branch as far as popliteal level. Of 83 patients discharged with patent prosthesis, thrombosis occurred in 7 and 10 reoperations were necessary. Reconstruction of branch patency was done in the majority of cases by thrombectomy. Profundaplasty was associated in 4 cases while in other 4 popliteal extension was necessary. Analysis of results shows that the operation of choice on the affluxion route seems to be replacement of the thrombosed branch.", "contents": "[Late obstructions of aorto-bifemoral prostheses]. The factors underlying late thrombosis of aortobifemoral by-pass are analysed on the basis of personal experience and reported data. They are considered to be due to progressive degradation of the prosthesic tissue and, particularly, to the lack of an adequate back flow route, apart from any faults in the actual construction of the femoral anastomosis. Treatment of the thrombotic complication involves considerable technical and operating problems which have two main solutions: substitution of the thrombosed branch, or its disobstruction. Intimately linked with restoral of by-pass patency is the reconstruction of an adequate back flow route either by means of profundaplasty or by extending the branch as far as popliteal level. Of 83 patients discharged with patent prosthesis, thrombosis occurred in 7 and 10 reoperations were necessary. Reconstruction of branch patency was done in the majority of cases by thrombectomy. Profundaplasty was associated in 4 cases while in other 4 popliteal extension was necessary. Analysis of results shows that the operation of choice on the affluxion route seems to be replacement of the thrombosed branch."}
{"id": "PMID:471291", "title": "[Value of the use of ultrasonics in the diagnosis of aneurysms of the abdominal aorta].", "content": "An account is given of the basic theory underlying the use of ultrasounds in the diagnosis of dilatation of the abdominal aorta, and its practical value. The limitations of physical examination and vacuum radiography, and the contraindications, inconveniences and risks associated with aortography are points in favour of echography. The salient features of its application in suspected aneurysm are described with reference to 12 clinical cases. This simple and harmless examination offers a reliable and non-invasive means of detecting the presence and determining the size of such aneurysms with safety and certainty.", "contents": "[Value of the use of ultrasonics in the diagnosis of aneurysms of the abdominal aorta]. An account is given of the basic theory underlying the use of ultrasounds in the diagnosis of dilatation of the abdominal aorta, and its practical value. The limitations of physical examination and vacuum radiography, and the contraindications, inconveniences and risks associated with aortography are points in favour of echography. The salient features of its application in suspected aneurysm are described with reference to 12 clinical cases. This simple and harmless examination offers a reliable and non-invasive means of detecting the presence and determining the size of such aneurysms with safety and certainty."}
{"id": "PMID:471292", "title": "[Arteriovenous fistulas for hemodialysis. Experience in 366 cases].", "content": "The surgical techniques adopted in performing 366 a-v fistulas for hemodialysis in 346 patients over ten years' experience are presented. A survey of the results obtained is then made as well as the evaluation of the same, based upon functional and clinical parameters. The technical details sometimes adopted, the occlusions and the principles drawn out for personal experience in this field are described.", "contents": "[Arteriovenous fistulas for hemodialysis. Experience in 366 cases]. The surgical techniques adopted in performing 366 a-v fistulas for hemodialysis in 346 patients over ten years' experience are presented. A survey of the results obtained is then made as well as the evaluation of the same, based upon functional and clinical parameters. The technical details sometimes adopted, the occlusions and the principles drawn out for personal experience in this field are described."}
{"id": "PMID:471293", "title": "[The superior mesenteric artery syndrome. Anatomo-clinical study and therapeutic possibilities in vascular compression of the duodenum].", "content": "Four cases of superiore mesenteric artery syndrome are presented. Reference is made to the difficulties involved in the clinical diagnosis of this form. It is pointed out that radiological examination is essential, since it dispel all doubts in nearly every case. Associations between the syndrome and peptic ulcer are relatively, and the joint surgical resolution of both forms is recommended.", "contents": "[The superior mesenteric artery syndrome. Anatomo-clinical study and therapeutic possibilities in vascular compression of the duodenum]. Four cases of superiore mesenteric artery syndrome are presented. Reference is made to the difficulties involved in the clinical diagnosis of this form. It is pointed out that radiological examination is essential, since it dispel all doubts in nearly every case. Associations between the syndrome and peptic ulcer are relatively, and the joint surgical resolution of both forms is recommended."}
{"id": "PMID:471305", "title": "[Preventive medicine in adults. Fundamentals and prospects].", "content": "The Author puts in evidence the need of a real development of the Medical Science, so to give the basis for a program of preventive medicine of the adult, that is prevention of degenerative pathology. A new way of thinking about this pathology starts from its relation with the aging process. This \"interpretative way\" may often be much more usefull than the mere diagnostic definition (as in the traditional medicine for the infectious disease) with its consequences of therapeutic activism and of a \"by any way gained\" normalization of biological parameters. Under this point of view one can look at the various aspects of the prevention. So the Author claims for a real evaluation of the risk factors and quite important, of the protective factors. These protective factors are probably of big value for preventive medical practice, as important as the health education. Actually people's health must be driven and saved much more than restored, a \"Utopia\" in the field of degenerative and senile pathology.", "contents": "[Preventive medicine in adults. Fundamentals and prospects]. The Author puts in evidence the need of a real development of the Medical Science, so to give the basis for a program of preventive medicine of the adult, that is prevention of degenerative pathology. A new way of thinking about this pathology starts from its relation with the aging process. This \"interpretative way\" may often be much more usefull than the mere diagnostic definition (as in the traditional medicine for the infectious disease) with its consequences of therapeutic activism and of a \"by any way gained\" normalization of biological parameters. Under this point of view one can look at the various aspects of the prevention. So the Author claims for a real evaluation of the risk factors and quite important, of the protective factors. These protective factors are probably of big value for preventive medical practice, as important as the health education. Actually people's health must be driven and saved much more than restored, a \"Utopia\" in the field of degenerative and senile pathology."}
{"id": "PMID:471306", "title": "[Sleep characteristics of elderly and very long-lived subjects].", "content": "Through the method of the \"questionnaire\" quantitative and qualitative aspects of the sleep of a group of 24 eldery people and of 24 very old people have been studied. Regarding the sleep of the eldery person the attention is recalled to two data: the presence of dreams of \"erotic\" contents in 50% of the subjects and the lack of a substancial difference between the agregate duration of sleep of the adult compared to that of the eldery person. About the sleep of the very old person the A.A. underlines the tendency to sleep more in old age: in this research, one hour more than the eldery. The very old people in the total of examined cases are people that, during life, have always gone to bed \"early\" in the evening. It is suggestive that only two out of 24 very old people have declared to have \"anguishing\" dreams.", "contents": "[Sleep characteristics of elderly and very long-lived subjects]. Through the method of the \"questionnaire\" quantitative and qualitative aspects of the sleep of a group of 24 eldery people and of 24 very old people have been studied. Regarding the sleep of the eldery person the attention is recalled to two data: the presence of dreams of \"erotic\" contents in 50% of the subjects and the lack of a substancial difference between the agregate duration of sleep of the adult compared to that of the eldery person. About the sleep of the very old person the A.A. underlines the tendency to sleep more in old age: in this research, one hour more than the eldery. The very old people in the total of examined cases are people that, during life, have always gone to bed \"early\" in the evening. It is suggestive that only two out of 24 very old people have declared to have \"anguishing\" dreams."}
{"id": "PMID:471365", "title": "Leiomyosarcoma of the uterus: case reports and a review.", "content": "Eight patients with leiomyosarcoma (LMS) of the uterus recently treated at our institution are reported. Modes of initial therapy in these patients included surgery alone, radiation therapy alone, or surgery plus radiation therapy. Mitotic counts were greater than 10 per 10 high power fields in the tumors of all of these patients. Only two patients are currently alive, and both have been treated for metastatic neoplasm to the lungs. A comparison of the histological material of these patients with criteria outlined in the recent literature for a diagnosis of uterine LMS is made. The possible role of a hormonal influence on this neoplasm is discussed. It would appear that a more aggressive initial therapy plan may improve survival in patients with this lethal malignancy.", "contents": "Leiomyosarcoma of the uterus: case reports and a review. Eight patients with leiomyosarcoma (LMS) of the uterus recently treated at our institution are reported. Modes of initial therapy in these patients included surgery alone, radiation therapy alone, or surgery plus radiation therapy. Mitotic counts were greater than 10 per 10 high power fields in the tumors of all of these patients. Only two patients are currently alive, and both have been treated for metastatic neoplasm to the lungs. A comparison of the histological material of these patients with criteria outlined in the recent literature for a diagnosis of uterine LMS is made. The possible role of a hormonal influence on this neoplasm is discussed. It would appear that a more aggressive initial therapy plan may improve survival in patients with this lethal malignancy."}
{"id": "PMID:471366", "title": "Changing trends and prognostic features in endometrial cancer associated with exogenous estrogen therapy.", "content": "A study was undertaken to investigate changing trends in the microscopic patterns of endometrial carcinoma and to compare the biologic characteristics of those cases associated with and without estrogen usage. After each case was reviewed independently and in a random order by at least 2 pathologists, a diagnosis of cancer was agreed on in 274 patients who had been treated by 5 gynecologists at the Masachusetts General Hospital between 1940 and 1971. Six microscopic patterns were identified (adenocarcinoma, adenoacanthoma, atypical adenoacanthoma, adenosquamous carcinoma, clear-cell adenocarcinoma, and undifferentiated carcinoma). The frequency of each pattern relative to the other 5 changed only slightly during the 30-year interval. The tumors that developed in estrogen users were more highly differentiated than those that developed in nonusers (P less than 0.005) and were found at an earlier average age (P less than 0.02). That the adenoacanthoma was associated with estrogen usage more frequently (51%) than any other tumor type (P less than 0.02) may reflect, in part, a similar and lower mean age of estrogen users (56 years) and patients with adenoacanthoma (55 years) compared with that of nonusers with the other forms of tumors (60--67 years). Although the overall 5- and 10-year survival rates of the estrogen users were higher than those of the nonusers, the differences between the 2 groups disappeared when the grade of the neoplasm was considered.", "contents": "Changing trends and prognostic features in endometrial cancer associated with exogenous estrogen therapy. A study was undertaken to investigate changing trends in the microscopic patterns of endometrial carcinoma and to compare the biologic characteristics of those cases associated with and without estrogen usage. After each case was reviewed independently and in a random order by at least 2 pathologists, a diagnosis of cancer was agreed on in 274 patients who had been treated by 5 gynecologists at the Masachusetts General Hospital between 1940 and 1971. Six microscopic patterns were identified (adenocarcinoma, adenoacanthoma, atypical adenoacanthoma, adenosquamous carcinoma, clear-cell adenocarcinoma, and undifferentiated carcinoma). The frequency of each pattern relative to the other 5 changed only slightly during the 30-year interval. The tumors that developed in estrogen users were more highly differentiated than those that developed in nonusers (P less than 0.005) and were found at an earlier average age (P less than 0.02). That the adenoacanthoma was associated with estrogen usage more frequently (51%) than any other tumor type (P less than 0.02) may reflect, in part, a similar and lower mean age of estrogen users (56 years) and patients with adenoacanthoma (55 years) compared with that of nonusers with the other forms of tumors (60--67 years). Although the overall 5- and 10-year survival rates of the estrogen users were higher than those of the nonusers, the differences between the 2 groups disappeared when the grade of the neoplasm was considered."}
{"id": "PMID:471367", "title": "The transverse vaginal septum: a clinicopathologic evaluation.", "content": "Twelve cases of transverse vaginal septum were clinically and pathologically evaluated. Seven cases were treated with electrosurgical excision of the septum with encouraging results. Histologic findings in the patients in this study support the view that the epithelium of the vagina and the transverse septum are of mesonephroid origin.", "contents": "The transverse vaginal septum: a clinicopathologic evaluation. Twelve cases of transverse vaginal septum were clinically and pathologically evaluated. Seven cases were treated with electrosurgical excision of the septum with encouraging results. Histologic findings in the patients in this study support the view that the epithelium of the vagina and the transverse septum are of mesonephroid origin."}
{"id": "PMID:471368", "title": "Fatal perineal cellulitis from an episiotomy site.", "content": "Perineal cellulitis originating from an episiotomy incision resulted in 20% of the maternal mortality in King County, Washington, between 1969 and 1977. Necrotizing fasciitis was present in 2 of the cases, and clostridial myonecrosis was present in 1. These fatalities occurred because the practitioners were not aware that necrotizing fasciitis can occur in the fatty superficial fascia of the perineum and that resection of the necrotic tissue is necessary for successful therapy.", "contents": "Fatal perineal cellulitis from an episiotomy site. Perineal cellulitis originating from an episiotomy incision resulted in 20% of the maternal mortality in King County, Washington, between 1969 and 1977. Necrotizing fasciitis was present in 2 of the cases, and clostridial myonecrosis was present in 1. These fatalities occurred because the practitioners were not aware that necrotizing fasciitis can occur in the fatty superficial fascia of the perineum and that resection of the necrotic tissue is necessary for successful therapy."}
{"id": "PMID:471369", "title": "A comparison of different methods of instrumental delivery based on electronic measurements of compression and traction.", "content": "Electronic measurements of compression, using a hydrostatic technique, were made during 44 normal deliveries and compared with the values obtained during 32 deliveries using Kielland's forceps, 21 using Neville Barnes' forceps, 48 using Moolgaoker's adjustable forceps, and 26 using Malmstrom's vacuum extractor. Electronic recordings of traction, using strain gauges, were made simultaneously during all the instrumental deliveries except those with Kielland's forceps. By exercising suitable controls over most of the multiple factors operating at the time of any delivery the authors were able to compare objectively the efficiency of the different methods of instrumental delivery. Smaller forces of compression and traction were exerted and better Apgar scores were recorded in infants delivered with the adjustable forceps than in infants delivered with the other instruments. The superiority of the adjustable forceps was most noticeable during midcavity deliveries of the malrotated head.", "contents": "A comparison of different methods of instrumental delivery based on electronic measurements of compression and traction. Electronic measurements of compression, using a hydrostatic technique, were made during 44 normal deliveries and compared with the values obtained during 32 deliveries using Kielland's forceps, 21 using Neville Barnes' forceps, 48 using Moolgaoker's adjustable forceps, and 26 using Malmstrom's vacuum extractor. Electronic recordings of traction, using strain gauges, were made simultaneously during all the instrumental deliveries except those with Kielland's forceps. By exercising suitable controls over most of the multiple factors operating at the time of any delivery the authors were able to compare objectively the efficiency of the different methods of instrumental delivery. Smaller forces of compression and traction were exerted and better Apgar scores were recorded in infants delivered with the adjustable forceps than in infants delivered with the other instruments. The superiority of the adjustable forceps was most noticeable during midcavity deliveries of the malrotated head."}
{"id": "PMID:471370", "title": "A paired controlled study of vaginal and abdominal delivery of the low birth weight breech fetus.", "content": "In a paired, controlled, retrospective study, 44 low birth weight breech fetuses delivered vaginally were compared with 44 breech fetuses delivered by cesarean section. Seven deaths occurred in the vaginal delivery group, compared with only 1 in the abdominal delivery group. Asphyxia, trauma, and intracranial hemorrhage were also found more frequently among vaginally delivered fetuses. The authors conclude that for the low birth weight breech fetus, delivery by cesarean section is preferable.", "contents": "A paired controlled study of vaginal and abdominal delivery of the low birth weight breech fetus. In a paired, controlled, retrospective study, 44 low birth weight breech fetuses delivered vaginally were compared with 44 breech fetuses delivered by cesarean section. Seven deaths occurred in the vaginal delivery group, compared with only 1 in the abdominal delivery group. Asphyxia, trauma, and intracranial hemorrhage were also found more frequently among vaginally delivered fetuses. The authors conclude that for the low birth weight breech fetus, delivery by cesarean section is preferable."}
{"id": "PMID:471371", "title": "hPL: physiologic and pathophysiologic observations.", "content": "Serum human placental lactogen (hPL) levels were studied in 806 women in late pregnancy. The hPL levels were positively correlated with birth weight but were unrelated to maternal age, parity, socioeconomic status, or the sex of the newborn. The hPL levels peaked at 37 weeks' gestation and then declined moderately. An individual's hPL levels in late pregnancy are quite constant week to week. Patients with severe chronic hypertension have low hPL values; those carrying twins have high values.", "contents": "hPL: physiologic and pathophysiologic observations. Serum human placental lactogen (hPL) levels were studied in 806 women in late pregnancy. The hPL levels were positively correlated with birth weight but were unrelated to maternal age, parity, socioeconomic status, or the sex of the newborn. The hPL levels peaked at 37 weeks' gestation and then declined moderately. An individual's hPL levels in late pregnancy are quite constant week to week. Patients with severe chronic hypertension have low hPL values; those carrying twins have high values."}
{"id": "PMID:471372", "title": "Glycosylated hemoglobins in normal pregnancy and gestational diabetes mellitus.", "content": "Glycosylated hemoglobins (HbA1) were measured in 23 nonpregnant women, 53 normal pregnant women, and 22 Class A diabetics; the results were 6.1 +/- 0.7%, 5.8 +/- 1.0%, and 7.0 +/- 1.3% in the 3 groups, respectively. The decrease in normal pregnancy was insignificant, whereas the increase in HbA1 in Class A diabetics over the other 2 groups was statistically significant (P less than 0.05). HbA1 did not correlate with maternal age, gravidity, or gestational age at the time of sampling. There was no difference in HbA1 between whites and blacks [patients with sickle hemoglobin (HbS) were excluded] (P = 0.35), nor between primigravidas and multigravidas (P = 0.8). HbA1 levels did not correlate with the birth weight ratios in either normal pregnancies (r = 0.06, P = 0.7) or in Class A diabetics (r = -0.4, P = 0.09). This is probably due to the long interval between HbA1 determination and delivery (9.9 weeks).", "contents": "Glycosylated hemoglobins in normal pregnancy and gestational diabetes mellitus. Glycosylated hemoglobins (HbA1) were measured in 23 nonpregnant women, 53 normal pregnant women, and 22 Class A diabetics; the results were 6.1 +/- 0.7%, 5.8 +/- 1.0%, and 7.0 +/- 1.3% in the 3 groups, respectively. The decrease in normal pregnancy was insignificant, whereas the increase in HbA1 in Class A diabetics over the other 2 groups was statistically significant (P less than 0.05). HbA1 did not correlate with maternal age, gravidity, or gestational age at the time of sampling. There was no difference in HbA1 between whites and blacks [patients with sickle hemoglobin (HbS) were excluded] (P = 0.35), nor between primigravidas and multigravidas (P = 0.8). HbA1 levels did not correlate with the birth weight ratios in either normal pregnancies (r = 0.06, P = 0.7) or in Class A diabetics (r = -0.4, P = 0.09). This is probably due to the long interval between HbA1 determination and delivery (9.9 weeks)."}
{"id": "PMID:471373", "title": "24-hour urine creatinine excretion in pregnancy.", "content": "A total of 489 consecutive 24-hour urine collection from 162 patients were analyzed for creatinine excretion. The 24-hour creatinine excretion increased with the urine volume. Creatinine excretions in 24-hour urine collections were not statistically different between in- and out-patients. The results indicate that the mean urine creatinine per 24 hours is 1.08 g for 24-hour urine volumes between 500 and 1500 ml. For urine volumes greater than 1500 ml it is 1.22 g. For 24-hour urine volumes less than 500 ml, the mean creatinine is 0.63 g.", "contents": "24-hour urine creatinine excretion in pregnancy. A total of 489 consecutive 24-hour urine collection from 162 patients were analyzed for creatinine excretion. The 24-hour creatinine excretion increased with the urine volume. Creatinine excretions in 24-hour urine collections were not statistically different between in- and out-patients. The results indicate that the mean urine creatinine per 24 hours is 1.08 g for 24-hour urine volumes between 500 and 1500 ml. For urine volumes greater than 1500 ml it is 1.22 g. For 24-hour urine volumes less than 500 ml, the mean creatinine is 0.63 g."}
{"id": "PMID:471374", "title": "Consolidation of hospital obstetric services--is it a reality?", "content": "Despite efforts toward regional planning of maternity services and consolidation of small hospital units, the number of US hospitals with maternity services has increased every year since 1973. Only 2 states have significantly reduced the number of hospitals performing less than 500 deliveries annually. The average number of annual deliveries per hospital is 728. No state has achieved an average number of deliveries as high as the 1500 deliveries called for in national guidelines for health planning.", "contents": "Consolidation of hospital obstetric services--is it a reality? Despite efforts toward regional planning of maternity services and consolidation of small hospital units, the number of US hospitals with maternity services has increased every year since 1973. Only 2 states have significantly reduced the number of hospitals performing less than 500 deliveries annually. The average number of annual deliveries per hospital is 728. No state has achieved an average number of deliveries as high as the 1500 deliveries called for in national guidelines for health planning."}
{"id": "PMID:471375", "title": "Vaginal epithelialization with human amnion.", "content": "Human amnion is a readily available and inexpensive allograft with low antigenicity, high antimicrobial potential, and the ability to foster epithelialization. It has been used to reconstruct the vagina in 2 patients following vaginectomy for diffuse carcinoma in situ (CIS) and in 1 patient following severe, corrosive, vulvovaginal burns. It was also used to construct the vagina in 1 patient with m\u00fcllerian agenesis. In all 4 cases, epithelialization was complete within 8 weeks.", "contents": "Vaginal epithelialization with human amnion. Human amnion is a readily available and inexpensive allograft with low antigenicity, high antimicrobial potential, and the ability to foster epithelialization. It has been used to reconstruct the vagina in 2 patients following vaginectomy for diffuse carcinoma in situ (CIS) and in 1 patient following severe, corrosive, vulvovaginal burns. It was also used to construct the vagina in 1 patient with m\u00fcllerian agenesis. In all 4 cases, epithelialization was complete within 8 weeks."}
{"id": "PMID:471376", "title": "Contact hysteroscopy: a new technique to explore the uterine cavity.", "content": "Contact hysteroscopy is a new endoscopic technique that allows direct examination of the uterine cavity. The procedure is performed using ambient room light, which is collected by a special light trap incorporated into the body of the instrument. The mechanism for gathering and transmitting light eliminates the need for any electric connections whatsoever. The optical design enables the examiner to see the endocervix and endometrium even in the presence of bleeding. Neither gas nor liquid media are required for observations since the uterine cavity is not distended. An initial appraisal of 80 women with the contact hysteroscope proved successful from the standpoint of technical simplicity, diagnostic accuracy, and absence of complications.", "contents": "Contact hysteroscopy: a new technique to explore the uterine cavity. Contact hysteroscopy is a new endoscopic technique that allows direct examination of the uterine cavity. The procedure is performed using ambient room light, which is collected by a special light trap incorporated into the body of the instrument. The mechanism for gathering and transmitting light eliminates the need for any electric connections whatsoever. The optical design enables the examiner to see the endocervix and endometrium even in the presence of bleeding. Neither gas nor liquid media are required for observations since the uterine cavity is not distended. An initial appraisal of 80 women with the contact hysteroscope proved successful from the standpoint of technical simplicity, diagnostic accuracy, and absence of complications."}
{"id": "PMID:471377", "title": "Preeclampsia and the patient with myasthenia gravis.", "content": "Three cases of myasthenia gravis in pregnancy are presented. In 2 cases there were moderately severe hypertension or preeclampsia and difficulty in controlling the myasthenia symptoms. The other case was uncomplicated. The possible relationship between myasthenia gravis and preeclampsia is discussed.", "contents": "Preeclampsia and the patient with myasthenia gravis. Three cases of myasthenia gravis in pregnancy are presented. In 2 cases there were moderately severe hypertension or preeclampsia and difficulty in controlling the myasthenia symptoms. The other case was uncomplicated. The possible relationship between myasthenia gravis and preeclampsia is discussed."}
{"id": "PMID:471378", "title": "Recurring oral pregnancy tumors.", "content": "Two cases of recurring \"pregnancy tumors\" are described. The likelihood of these lesions occurring and recurring following conservative therapy is enhanced by hormonal concentrations in the gingival tissues in conjunction with local factors produced by poor oral hygiene. This condition may pose a clinical problem in the pregnant woman.", "contents": "Recurring oral pregnancy tumors. Two cases of recurring \"pregnancy tumors\" are described. The likelihood of these lesions occurring and recurring following conservative therapy is enhanced by hormonal concentrations in the gingival tissues in conjunction with local factors produced by poor oral hygiene. This condition may pose a clinical problem in the pregnant woman."}
{"id": "PMID:471379", "title": "The oxytocin challenge test: an ominous pattern associated with severe fetal growth retardation.", "content": "Four cases of a rare atypical fetal heart pattern obtained during the oxytocin challenge test are described. In all cases, there were marked oligohydramnios and fetal growth retardation with a severely compromised fetus, and in 3 cases the fetuses died.", "contents": "The oxytocin challenge test: an ominous pattern associated with severe fetal growth retardation. Four cases of a rare atypical fetal heart pattern obtained during the oxytocin challenge test are described. In all cases, there were marked oligohydramnios and fetal growth retardation with a severely compromised fetus, and in 3 cases the fetuses died."}
{"id": "PMID:471380", "title": "The use of quinidine sulfate throughout pregnancy.", "content": "A gravid patient was given quinidine sulfate for idiopathic ventricular tachycardia. The serum levels of quinidine remained stable throughout pregnancy. At delivery, maternal and neonatal quinidine levels were equivalent, while the level in the amniotic fluid was significantly elevated. Explanations for the latter include 1) a concentration of quinidine in amniotic fluid due to spontaneous fetal voiding and 2) the use of a nonspecific protein precipitate method to measure quinidine that is unable to differentiate quinidine from its nonfunctioning metabolites. The diffusion of quinidine into breast milk was related to the serum quinidine concentration.", "contents": "The use of quinidine sulfate throughout pregnancy. A gravid patient was given quinidine sulfate for idiopathic ventricular tachycardia. The serum levels of quinidine remained stable throughout pregnancy. At delivery, maternal and neonatal quinidine levels were equivalent, while the level in the amniotic fluid was significantly elevated. Explanations for the latter include 1) a concentration of quinidine in amniotic fluid due to spontaneous fetal voiding and 2) the use of a nonspecific protein precipitate method to measure quinidine that is unable to differentiate quinidine from its nonfunctioning metabolites. The diffusion of quinidine into breast milk was related to the serum quinidine concentration."}
{"id": "PMID:471381", "title": "Hydranencephaly in a twin gestation.", "content": "Hydranencephaly in a twin gestation was demonstrated prenatally by ultrasonography and confirmed following delivery by cranial computerized axial tomography (CAT). Prenatal diagnosis of intracranial anomalies is possible with the use of ultrasonography. This is the third report of the occurrence of hydranencephaly in a twin gestation.", "contents": "Hydranencephaly in a twin gestation. Hydranencephaly in a twin gestation was demonstrated prenatally by ultrasonography and confirmed following delivery by cranial computerized axial tomography (CAT). Prenatal diagnosis of intracranial anomalies is possible with the use of ultrasonography. This is the third report of the occurrence of hydranencephaly in a twin gestation."}
{"id": "PMID:471382", "title": "Diagnosis of congenital mitral and aortic stenosis from the fetal electrocardiogram.", "content": "A case of congenital mitral and aortic stenosis diagnosed from the fetal electrocardiogram (FECG) during labor is presented. Cardiologic work-up and autopsy findings in the neonate confirmed the diagnosis. This is the first case in which prenatal diagnosis of a specific anatomical lesion of the heart was made from the FECG and confirmed clinically as well as at autopsy.", "contents": "Diagnosis of congenital mitral and aortic stenosis from the fetal electrocardiogram. A case of congenital mitral and aortic stenosis diagnosed from the fetal electrocardiogram (FECG) during labor is presented. Cardiologic work-up and autopsy findings in the neonate confirmed the diagnosis. This is the first case in which prenatal diagnosis of a specific anatomical lesion of the heart was made from the FECG and confirmed clinically as well as at autopsy."}
{"id": "PMID:471383", "title": "Cerebral abscess associated with an intrauterine contraceptive device.", "content": "The present communication is the first report of a metastatic brain abscess occurring as a complication of severe intrauterine device (IUD)-related pelvic inflammatory disease (PID). The diagnostic and therapeutic problems are discussed, and a suggestion is made for the reporting of all IUD-associated infections to a central agency.", "contents": "Cerebral abscess associated with an intrauterine contraceptive device. The present communication is the first report of a metastatic brain abscess occurring as a complication of severe intrauterine device (IUD)-related pelvic inflammatory disease (PID). The diagnostic and therapeutic problems are discussed, and a suggestion is made for the reporting of all IUD-associated infections to a central agency."}
{"id": "PMID:471384", "title": "Postpartum hypokalemic paralysis.", "content": "An attack of hypokalemic paralysis occurring in a 23-year-old woman during the immediate postpartum period of her fourth pregnancy is described. A flaccid paralysis of her extremities and markedly diminished deep tendon reflexes developed 3 hours after delivery, at which time her serum potassium level was found to be 1.7 mEq/liter. Hypokalemic paralysis has not been previously reported as an immediate postpartum complication. The pathophysiology and management of this condition are discussed, as are the treatment and follow-up of this specific patient.", "contents": "Postpartum hypokalemic paralysis. An attack of hypokalemic paralysis occurring in a 23-year-old woman during the immediate postpartum period of her fourth pregnancy is described. A flaccid paralysis of her extremities and markedly diminished deep tendon reflexes developed 3 hours after delivery, at which time her serum potassium level was found to be 1.7 mEq/liter. Hypokalemic paralysis has not been previously reported as an immediate postpartum complication. The pathophysiology and management of this condition are discussed, as are the treatment and follow-up of this specific patient."}
{"id": "PMID:471385", "title": "Fetus papyraceus causing dystocia: inability to detect blighted twin antenatally.", "content": "A case is reported of fetus papyraceus diagnosed intrapartum. There was no clinical evidence in early and midpregnancy to suggest such a diagnosis. Ultrasound examination performed 1 week prior to delivery was nondiagnostic, but an x-ray taken intrapartum revealed dystocia caused by fetus papyraceus, thus necessitating cesarean section. It is suggested that the clinician consider x-ray as well as ultrasound in the diagnosis of fetus papyraceus. In addition to the obstetric morbidity, there is considerable neonatal morbidity which further increases the importance of antenatal diagnosis of fetus papyraceus.", "contents": "Fetus papyraceus causing dystocia: inability to detect blighted twin antenatally. A case is reported of fetus papyraceus diagnosed intrapartum. There was no clinical evidence in early and midpregnancy to suggest such a diagnosis. Ultrasound examination performed 1 week prior to delivery was nondiagnostic, but an x-ray taken intrapartum revealed dystocia caused by fetus papyraceus, thus necessitating cesarean section. It is suggested that the clinician consider x-ray as well as ultrasound in the diagnosis of fetus papyraceus. In addition to the obstetric morbidity, there is considerable neonatal morbidity which further increases the importance of antenatal diagnosis of fetus papyraceus."}
{"id": "PMID:471386", "title": "Uterine artery ligation for postabortal hemorrhage.", "content": "A case is presented that demonstrates that bilateral ligation of the ascending branches of the uterine artery to control uterine hemorrhage is as effective in the first trimester as it is in the last trimester.", "contents": "Uterine artery ligation for postabortal hemorrhage. A case is presented that demonstrates that bilateral ligation of the ascending branches of the uterine artery to control uterine hemorrhage is as effective in the first trimester as it is in the last trimester."}
{"id": "PMID:471387", "title": "A giant fibroid uterus.", "content": "Giant uterine fibroids are those weighing greater than 25 lb (11.4 kg). Such a fibroid may appear as a solitary tumor or as a conglomerate mass of uterine fibroids presenting as a single uterine tumor. The challenge of these tumors lies in their proper surgical management. Combined preoperative and postoperative mortality is approximately 15%. The paucity of recent reports encountered in the literature of uterine fibroids of this magnitude reflects their rarity.", "contents": "A giant fibroid uterus. Giant uterine fibroids are those weighing greater than 25 lb (11.4 kg). Such a fibroid may appear as a solitary tumor or as a conglomerate mass of uterine fibroids presenting as a single uterine tumor. The challenge of these tumors lies in their proper surgical management. Combined preoperative and postoperative mortality is approximately 15%. The paucity of recent reports encountered in the literature of uterine fibroids of this magnitude reflects their rarity."}
{"id": "PMID:471388", "title": "Gonadal dysgenesis with vulvar carcinoma.", "content": "An unusual case of vulvar carcinoma in a gonadal dysgenesis patient is described. In addition to clinical manifestations, certain laboratory data related to endocrine status were gathered from serum and urine analyses. Finally, receptors for estrogens, progestins, and glucocorticoids were determined on biopsies of the vulvar carcinoma.", "contents": "Gonadal dysgenesis with vulvar carcinoma. An unusual case of vulvar carcinoma in a gonadal dysgenesis patient is described. In addition to clinical manifestations, certain laboratory data related to endocrine status were gathered from serum and urine analyses. Finally, receptors for estrogens, progestins, and glucocorticoids were determined on biopsies of the vulvar carcinoma."}
{"id": "PMID:471389", "title": "Hindgut duplication with rectovaginal fistula.", "content": "Complete colon duplication is an extremely rare congenital anomaly that occasionally presents diagnostic problems. This report presents a 23-year-old black woman with complete duplication of the colon and distal ileum, with termination of 1 colon into the vagina.", "contents": "Hindgut duplication with rectovaginal fistula. Complete colon duplication is an extremely rare congenital anomaly that occasionally presents diagnostic problems. This report presents a 23-year-old black woman with complete duplication of the colon and distal ileum, with termination of 1 colon into the vagina."}
{"id": "PMID:471390", "title": "Cephalothoracopagus (Janiceps) twinning.", "content": "Conjoined twins occur with a frequency of 1/50,000--1/100,000 deliveries. The cephalothoracopagus variety is particularly rare. This paper presents a case report of cephalothoracopagus (Janiceps) twinning, with a detailed pathologic analysis, as well as an historical review and discussion of diagnostic modalities.", "contents": "Cephalothoracopagus (Janiceps) twinning. Conjoined twins occur with a frequency of 1/50,000--1/100,000 deliveries. The cephalothoracopagus variety is particularly rare. This paper presents a case report of cephalothoracopagus (Janiceps) twinning, with a detailed pathologic analysis, as well as an historical review and discussion of diagnostic modalities."}
{"id": "PMID:471422", "title": "Microspectrofluorometric analysis of surface antigens of murine melanoma and hamster peritoneal cell hybrids: comparisons of species antigenicity, chromosome number, and tumorigenicity.", "content": "Somatic cell hybrids from viral fusions of murine melanoma (PAZG) X Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6%) CH chromosome loss but 80%PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and one PAZG cell, lost 20% of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells.", "contents": "Microspectrofluorometric analysis of surface antigens of murine melanoma and hamster peritoneal cell hybrids: comparisons of species antigenicity, chromosome number, and tumorigenicity. Somatic cell hybrids from viral fusions of murine melanoma (PAZG) X Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6%) CH chromosome loss but 80%PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and one PAZG cell, lost 20% of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells."}
{"id": "PMID:471423", "title": "A possible association of pernicious anemia with neoplasia.", "content": "7 out of 39 patients with pernicious anemia developed 9 different neoplasms during a period of 3--20 years after the diagnosis of pernicious anemia. These primary neoplasms originated from the lymph nodes, larynx, colon, stomach, kidney, meninges, maxillary sinus and eighth nerve. Treatment with vitamin B12 did not influence the development of tumor. Statistical analysis showed that the observed incidence of 9 neoplasms in this group was significantly higher than the expected 3.3 cases during the aggregate follow-up period (p = 0.002). Although a higher incidence of neoplasms in patients with other underlying diseases does not necessarily indicate an association, a high degree of suspicion for neoplastic disease is justifiable in patients with pernicious anemia.", "contents": "A possible association of pernicious anemia with neoplasia. 7 out of 39 patients with pernicious anemia developed 9 different neoplasms during a period of 3--20 years after the diagnosis of pernicious anemia. These primary neoplasms originated from the lymph nodes, larynx, colon, stomach, kidney, meninges, maxillary sinus and eighth nerve. Treatment with vitamin B12 did not influence the development of tumor. Statistical analysis showed that the observed incidence of 9 neoplasms in this group was significantly higher than the expected 3.3 cases during the aggregate follow-up period (p = 0.002). Although a higher incidence of neoplasms in patients with other underlying diseases does not necessarily indicate an association, a high degree of suspicion for neoplastic disease is justifiable in patients with pernicious anemia."}
{"id": "PMID:471424", "title": "N-acetyltransferase activity as a possible criterion in prognosing the anti-tumor effect of cyclophosphamide.", "content": "The results obtained in the present study indicated that a reduction in the activity of N-acetyltransferase (EC 2.3.1.5) occurs in cyclophosphamide-treated mice with sarcoma 37. A difference in variations of the level of N-acetyltransferase activity in outbred mice with and without tumors was observed in cyclophosphamide-treated animals. It was established that the effect of cyclophosphamide on the activity of N-acetyltransferase depends on the initial level of the enzyme activity (phenotype of acetylation) as well as on the genotype which determines the N-acetyltransferase activity.", "contents": "N-acetyltransferase activity as a possible criterion in prognosing the anti-tumor effect of cyclophosphamide. The results obtained in the present study indicated that a reduction in the activity of N-acetyltransferase (EC 2.3.1.5) occurs in cyclophosphamide-treated mice with sarcoma 37. A difference in variations of the level of N-acetyltransferase activity in outbred mice with and without tumors was observed in cyclophosphamide-treated animals. It was established that the effect of cyclophosphamide on the activity of N-acetyltransferase depends on the initial level of the enzyme activity (phenotype of acetylation) as well as on the genotype which determines the N-acetyltransferase activity."}
{"id": "PMID:471425", "title": "Serum long-chain alcohol levels in healthy individuals and in patients with breast cancer.", "content": "Total long-chain alcohols were analyzed in blood sera from normal individuals and patients with diagnosed breast cancer. Tetra-, hexa- and octadecan-1-ol were the major long-chain alcohols detected in both groups. While the qualitative composition of the serum alcohols was similar in the two groups the average alcohol content of the serum of the breast cancer patients was approximately six times greater than that of the normal group. This difference in serum alcohol levels between the two groups was significant at p less than 0.01.", "contents": "Serum long-chain alcohol levels in healthy individuals and in patients with breast cancer. Total long-chain alcohols were analyzed in blood sera from normal individuals and patients with diagnosed breast cancer. Tetra-, hexa- and octadecan-1-ol were the major long-chain alcohols detected in both groups. While the qualitative composition of the serum alcohols was similar in the two groups the average alcohol content of the serum of the breast cancer patients was approximately six times greater than that of the normal group. This difference in serum alcohol levels between the two groups was significant at p less than 0.01."}
{"id": "PMID:471426", "title": "Chemotherapeutic agents and estradiol receptor binding capacity.", "content": "Cytotoxic agents used for the treatment of breast cancer did not inhibit the growth of uterus following estradiol injection to ovariectomized rats. They had no effect on the estradiol binding ability and on the dissociation constant of estradiol receptor binding of rat uterus. In addition, 1,2:5,6-dianhydrogalactitol, a highly active, proximal alkylating agent, applied in vitro did not alter the relative binding ability to tamoxifen to the cytoplasmic estradiol receptor of rat uterus and human breast cancer tissue. In summary, the present experiments did not reveal any disadvantageous interaction between simultaneously administered hormonal and cytotoxic drugs.", "contents": "Chemotherapeutic agents and estradiol receptor binding capacity. Cytotoxic agents used for the treatment of breast cancer did not inhibit the growth of uterus following estradiol injection to ovariectomized rats. They had no effect on the estradiol binding ability and on the dissociation constant of estradiol receptor binding of rat uterus. In addition, 1,2:5,6-dianhydrogalactitol, a highly active, proximal alkylating agent, applied in vitro did not alter the relative binding ability to tamoxifen to the cytoplasmic estradiol receptor of rat uterus and human breast cancer tissue. In summary, the present experiments did not reveal any disadvantageous interaction between simultaneously administered hormonal and cytotoxic drugs."}
{"id": "PMID:471427", "title": "Positive correlation between breast cancer incidence and HLA antigens.", "content": "HLA determinations were made at the time of diagnosis among a lot of 141 patients with breast cancer. Values of relative risk as well as frequencies of antigens at the first and the second locus were confronted to data found in a control population. Subdivisions of the neoplastic population according to hormonal status and parity led to the emergence of significant differences: frequency of A28 in menopausal nulliparous women with breast cancer is 26 vs. 7% in controls (p corrected = 0.038) with relative risk = 6.63 (p 0.001).", "contents": "Positive correlation between breast cancer incidence and HLA antigens. HLA determinations were made at the time of diagnosis among a lot of 141 patients with breast cancer. Values of relative risk as well as frequencies of antigens at the first and the second locus were confronted to data found in a control population. Subdivisions of the neoplastic population according to hormonal status and parity led to the emergence of significant differences: frequency of A28 in menopausal nulliparous women with breast cancer is 26 vs. 7% in controls (p corrected = 0.038) with relative risk = 6.63 (p 0.001)."}
{"id": "PMID:471428", "title": "Potentiating role of previously administered agents in the combination chemotherapy of breast cancer.", "content": "91 women with metastatic breast cancer, who received prior CTX-5FU or CTX-5FU-PRD, were treated in 3 consecutive clinical trials with either CTX-5FU-PRD-MTX-VCR, MTX-VCR, or MTX-VCR-PRD in order to elucidate whether the effectiveness of 5 drugs was due only to the newly added drugs (MTX-VCR +/- PRD) or whether the previously used drugs (CTX-5FU) were necessary as potentiating agents. There were 17 or 39 responders (43%) in the 5-drug group, 3 of 25 (12%) in the MTX-VCR group and 3 of 27 (11%) in the MTX-VCR-PRD group. The survival in the group treated with 5 drugs was significantly longer. Our results show that CTX-5FU-PRD-MTX-VCR was significantly more effective than MTX-VCR or MTX-VCR-PRD used alone after the patient had already had CTX-5FU +/- PRD. Thus, the previously used drugs (CTX-5FU) seemed to improve the response to the new agents (MTX-VCR +/- PRD) by a potentiating action in the subsequent combination. It is concluded that the exploitation of this enhancing effect represents a significant improvement in the treatment options now available.", "contents": "Potentiating role of previously administered agents in the combination chemotherapy of breast cancer. 91 women with metastatic breast cancer, who received prior CTX-5FU or CTX-5FU-PRD, were treated in 3 consecutive clinical trials with either CTX-5FU-PRD-MTX-VCR, MTX-VCR, or MTX-VCR-PRD in order to elucidate whether the effectiveness of 5 drugs was due only to the newly added drugs (MTX-VCR +/- PRD) or whether the previously used drugs (CTX-5FU) were necessary as potentiating agents. There were 17 or 39 responders (43%) in the 5-drug group, 3 of 25 (12%) in the MTX-VCR group and 3 of 27 (11%) in the MTX-VCR-PRD group. The survival in the group treated with 5 drugs was significantly longer. Our results show that CTX-5FU-PRD-MTX-VCR was significantly more effective than MTX-VCR or MTX-VCR-PRD used alone after the patient had already had CTX-5FU +/- PRD. Thus, the previously used drugs (CTX-5FU) seemed to improve the response to the new agents (MTX-VCR +/- PRD) by a potentiating action in the subsequent combination. It is concluded that the exploitation of this enhancing effect represents a significant improvement in the treatment options now available."}
{"id": "PMID:471429", "title": "Pleural effusion secondary to tumor regression.", "content": "2 patients with metastatic cancer (Ewing's sarcoma and breast cancer) developed pleural effusions while undergoing systemic therapy for pulmonary metastases. Thoracenteses failed to reveal malignant cells and in both instances the characteristics of the fluids were those of transudates. The effusions developed in association with dissolution of pulmonary lesions and presumably represent a reactive process to tumor regression.", "contents": "Pleural effusion secondary to tumor regression. 2 patients with metastatic cancer (Ewing's sarcoma and breast cancer) developed pleural effusions while undergoing systemic therapy for pulmonary metastases. Thoracenteses failed to reveal malignant cells and in both instances the characteristics of the fluids were those of transudates. The effusions developed in association with dissolution of pulmonary lesions and presumably represent a reactive process to tumor regression."}
{"id": "PMID:471430", "title": "[Osteogenic aneurysmal orbital cysts].", "content": "The orbital form of this osseous tumor-like dystrophy is quite rare. The illustrated description of a new case is reported and the 13 cases of the literature reviewed. The main clinical and radiological data as well as the different treatments are commented upon. The anatomo-pathologic discussion focuses on the morphological aspects, hypotheses on the pathogenesis and differential microscopic features of the lesion as compared to other osseous lesions of the orbital bones.", "contents": "[Osteogenic aneurysmal orbital cysts]. The orbital form of this osseous tumor-like dystrophy is quite rare. The illustrated description of a new case is reported and the 13 cases of the literature reviewed. The main clinical and radiological data as well as the different treatments are commented upon. The anatomo-pathologic discussion focuses on the morphological aspects, hypotheses on the pathogenesis and differential microscopic features of the lesion as compared to other osseous lesions of the orbital bones."}
{"id": "PMID:471431", "title": "Rare association of hyperglycinuria and lenticonus in two members of the same family.", "content": "2 cases of posterior lenticonus associated with hyperglycinuria without iminoaciduria, found in the same family, were examined. After a review of the literature, the rarity of this association is stressed.", "contents": "Rare association of hyperglycinuria and lenticonus in two members of the same family. 2 cases of posterior lenticonus associated with hyperglycinuria without iminoaciduria, found in the same family, were examined. After a review of the literature, the rarity of this association is stressed."}
{"id": "PMID:471432", "title": "[Risk of transmission of hepatitis B through contact lens fitting set].", "content": "8 out of 19 cases of acute or chronic hepatitis, with positive hepatitis B surface antigen (HBsAg) had HBsAg-positive tears. In 25 healthy HBsAg carriers, no HBsAg was found in the tears. The HBsAg concentration in the tears of hepatitis patients was less than 1% of the HBsAg in their blood. Contact lenses (soft hydrophilic, silicone) experimentally contaminated with HBsAg were practically free of it after the usual cleaning and washing with a commercial contact lens cleaning fluid. No adsorption of HBsAg was found on hydrophilic soft lenses; a minimal adsorption on silicone lenses could not be entirely excluded, but practically it was without any importance. In 14 samples of 79 storage fluids of a contact lens fitting set HBsAg was found. The cause of this contamination is not clear.", "contents": "[Risk of transmission of hepatitis B through contact lens fitting set]. 8 out of 19 cases of acute or chronic hepatitis, with positive hepatitis B surface antigen (HBsAg) had HBsAg-positive tears. In 25 healthy HBsAg carriers, no HBsAg was found in the tears. The HBsAg concentration in the tears of hepatitis patients was less than 1% of the HBsAg in their blood. Contact lenses (soft hydrophilic, silicone) experimentally contaminated with HBsAg were practically free of it after the usual cleaning and washing with a commercial contact lens cleaning fluid. No adsorption of HBsAg was found on hydrophilic soft lenses; a minimal adsorption on silicone lenses could not be entirely excluded, but practically it was without any importance. In 14 samples of 79 storage fluids of a contact lens fitting set HBsAg was found. The cause of this contamination is not clear."}
{"id": "PMID:471433", "title": "Naevus of the choroid.", "content": "Despite their common occurrence and benign character, choroidal naevi show a number of interesting features. Hyper- and hypopigmentation, drusen, pigment-epithelial detachment, retinal deposits and capillary circulatory disturbances are described. Colour and red-light photography appear to be the most appropriate techniques for documentation.", "contents": "Naevus of the choroid. Despite their common occurrence and benign character, choroidal naevi show a number of interesting features. Hyper- and hypopigmentation, drusen, pigment-epithelial detachment, retinal deposits and capillary circulatory disturbances are described. Colour and red-light photography appear to be the most appropriate techniques for documentation."}
{"id": "PMID:471434", "title": "Eye position and electromyographic observation of squint eyes under general anesthesia.", "content": "The position of eyes under general anesthesia was measured in different groups of nonparalytic strabismus. Almost all patients with esotropia or hyperactive inferior oblique muscle showed divergent eye positions while patients with exotropia showed no consistent trend. The eye position of patients with lid ptosis without squint, which served as a control group, were divergent in all cases. Electromyographic observation of the medial rectus muscle under the same condition of anesthesia revealed that muscle discharge disappeared almost completely at the stage at which the eyes were in a well-stabilized position.", "contents": "Eye position and electromyographic observation of squint eyes under general anesthesia. The position of eyes under general anesthesia was measured in different groups of nonparalytic strabismus. Almost all patients with esotropia or hyperactive inferior oblique muscle showed divergent eye positions while patients with exotropia showed no consistent trend. The eye position of patients with lid ptosis without squint, which served as a control group, were divergent in all cases. Electromyographic observation of the medial rectus muscle under the same condition of anesthesia revealed that muscle discharge disappeared almost completely at the stage at which the eyes were in a well-stabilized position."}
{"id": "PMID:471499", "title": "The effects of impulse noise on the eardrum and middle ear.", "content": "Noise rarely damages the tympanic membrane and middle ear. High intensity impulse noise (explosions) can rupture the eardrum and disrupt the ossicular chain. Middle ear muscles play no part in the defense of the inner ear against high intensity impulse noise. Other mechanisms that protect the inner ear are slippage of the incudomalleal joint, a maximal limit of stapes displacement, and unlinking of the ossicular chain.", "contents": "The effects of impulse noise on the eardrum and middle ear. Noise rarely damages the tympanic membrane and middle ear. High intensity impulse noise (explosions) can rupture the eardrum and disrupt the ossicular chain. Middle ear muscles play no part in the defense of the inner ear against high intensity impulse noise. Other mechanisms that protect the inner ear are slippage of the incudomalleal joint, a maximal limit of stapes displacement, and unlinking of the ossicular chain."}
{"id": "PMID:471500", "title": "Sociopsychological factors affecting the human response to noise exposure.", "content": "Community noise is reported to be the most often mentioned undesirable neighborhood condition in a recent U.S. Census survey. Understanding community response to noise involves the measurement of a number of complex acoustic and nonacoustic variables and establishing the chain of relationships between physical exposure, perception, annoyance, and acceptability responses and finally complaint behavior. The perceived loudness of a noise is the most important acoustic parameter influencing annoyance and complaints, and the simple dBA unit can be used to integrate spectral characteristics of complex sounds in community studies. Although energy averaging such as Leq or Ldn can be used to describe multiple noise exposures over time, the variable trade-off relationships between number and level of exposures are somewhat obscured by such summary measures. However, they are still the best available descriptors and, until more accurate ones are developed, can be used to measure community noise environments. Perception of an identical noise exposure can vary according to the physiological noise sensitivity of a person and the activity context in which the noise is heard. Although the acoustic quality of the noise itself usually explains about 10 to 25 per cent of the variability in annoyance responses, sociopsychological variables measured in field studies account for 35 to 50 per cent of the variations in human annoyance responses. Three of the most important nonacoustic factors are the connotative fear effects of the noise signal, the feeling that those responsible for the noise are misfeasant in not reducing the noise, and the feeling that harmful health effects are produced by the noise. When residents report great fear, a high misfeasance, and marked health effects, about 90 per cent report a high annoyance level whether their noise exposure level is above 90 Ldn or 65 to 70 Ldn. In contrast, if the feelings are a low fear level, a low degree of misfeasance, and minimal health effects, only 3 to 10 per cent give high annoyance responses. Complaint behavior, although based on intensity of annoyance, is modified by a number of other sociopsychological factors and often is an unreliable indicator of an environmental problem.", "contents": "Sociopsychological factors affecting the human response to noise exposure. Community noise is reported to be the most often mentioned undesirable neighborhood condition in a recent U.S. Census survey. Understanding community response to noise involves the measurement of a number of complex acoustic and nonacoustic variables and establishing the chain of relationships between physical exposure, perception, annoyance, and acceptability responses and finally complaint behavior. The perceived loudness of a noise is the most important acoustic parameter influencing annoyance and complaints, and the simple dBA unit can be used to integrate spectral characteristics of complex sounds in community studies. Although energy averaging such as Leq or Ldn can be used to describe multiple noise exposures over time, the variable trade-off relationships between number and level of exposures are somewhat obscured by such summary measures. However, they are still the best available descriptors and, until more accurate ones are developed, can be used to measure community noise environments. Perception of an identical noise exposure can vary according to the physiological noise sensitivity of a person and the activity context in which the noise is heard. Although the acoustic quality of the noise itself usually explains about 10 to 25 per cent of the variability in annoyance responses, sociopsychological variables measured in field studies account for 35 to 50 per cent of the variations in human annoyance responses. Three of the most important nonacoustic factors are the connotative fear effects of the noise signal, the feeling that those responsible for the noise are misfeasant in not reducing the noise, and the feeling that harmful health effects are produced by the noise. When residents report great fear, a high misfeasance, and marked health effects, about 90 per cent report a high annoyance level whether their noise exposure level is above 90 Ldn or 65 to 70 Ldn. In contrast, if the feelings are a low fear level, a low degree of misfeasance, and minimal health effects, only 3 to 10 per cent give high annoyance responses. Complaint behavior, although based on intensity of annoyance, is modified by a number of other sociopsychological factors and often is an unreliable indicator of an environmental problem."}
{"id": "PMID:471502", "title": "An introduction to the measurement of sound.", "content": "The need for greater interest and participation in hearing conservation on the part of otolaryngologists, audiologists, and others is growing. Ever increasing numbers of people are being handicapped by noise induced hearing impairment. This costly damage is not curable but is preventable. Complaints of noise or annoyance must be investigated, and the primary tool for investigation is the sound level meter. It can be used by anyone with some basic knowledge to identify hazardous noise levels. Understanding the parts of the meter, the standards governing its construction, the basic steps in reading hazardous noise levels, including sources of error, methods of reporting the readings and knowing where expert assistance is available form the basis for monitoring hazardous noise levels. The following references contain material that can be used for additional study and sources of professional help. The number of publications dealing with noise has, as with the number of instruments and companies dealing with noise, risen sharply in the last three years. The sources listed constitute only a small portion of those available. These are selected for their detailed information, or their concrete and practical approach in providing immediately useful information, and for their usefulness as sources for further study materials or help with your hearing conservation program.", "contents": "An introduction to the measurement of sound. The need for greater interest and participation in hearing conservation on the part of otolaryngologists, audiologists, and others is growing. Ever increasing numbers of people are being handicapped by noise induced hearing impairment. This costly damage is not curable but is preventable. Complaints of noise or annoyance must be investigated, and the primary tool for investigation is the sound level meter. It can be used by anyone with some basic knowledge to identify hazardous noise levels. Understanding the parts of the meter, the standards governing its construction, the basic steps in reading hazardous noise levels, including sources of error, methods of reporting the readings and knowing where expert assistance is available form the basis for monitoring hazardous noise levels. The following references contain material that can be used for additional study and sources of professional help. The number of publications dealing with noise has, as with the number of instruments and companies dealing with noise, risen sharply in the last three years. The sources listed constitute only a small portion of those available. These are selected for their detailed information, or their concrete and practical approach in providing immediately useful information, and for their usefulness as sources for further study materials or help with your hearing conservation program."}
{"id": "PMID:471505", "title": "The economic impact of noise control.", "content": "This article has briefly summarized the cost of noise control for two standards under consideration by OSHA. Readers are encouraged to find ways to protect the hearing of workers in any way it can be done.", "contents": "The economic impact of noise control. This article has briefly summarized the cost of noise control for two standards under consideration by OSHA. Readers are encouraged to find ways to protect the hearing of workers in any way it can be done."}
{"id": "PMID:471506", "title": "Industrial hearing conservation.", "content": "Hearing conservation programs, when appropriate in industry, are now a necessity. Even though one may not wish to adopt an altruistic attitude toward the conservation of hearing, one must, like it or not, initiate a hearing conservation program because of both federal and state regulations. Since industrial noise exposure produced more hearing loss in more people than all other causes of hearing loss combined, it is incumbent on all industries with noise makers to do something about protecting human hearing. The tragedy is that nearly all industrial hearing loss can be prevented with proper hearing conservation measures. The cost of hearing conservation is far less than the cost of hearing loss in terms of human suffering and dollars in the compensation courts. Proper education of both managment and labor can result in successful hearing conservation programs. The method of choice is reduction of the noise at the source, but in many cases this is infeasible both technically and economically and therefore protection at the ear must be used. Experience has shown that with proper supervision ear protection programs can prevent the majority of instances of hearing loss in the majority of individuals exposed.", "contents": "Industrial hearing conservation. Hearing conservation programs, when appropriate in industry, are now a necessity. Even though one may not wish to adopt an altruistic attitude toward the conservation of hearing, one must, like it or not, initiate a hearing conservation program because of both federal and state regulations. Since industrial noise exposure produced more hearing loss in more people than all other causes of hearing loss combined, it is incumbent on all industries with noise makers to do something about protecting human hearing. The tragedy is that nearly all industrial hearing loss can be prevented with proper hearing conservation measures. The cost of hearing conservation is far less than the cost of hearing loss in terms of human suffering and dollars in the compensation courts. Proper education of both managment and labor can result in successful hearing conservation programs. The method of choice is reduction of the noise at the source, but in many cases this is infeasible both technically and economically and therefore protection at the ear must be used. Experience has shown that with proper supervision ear protection programs can prevent the majority of instances of hearing loss in the majority of individuals exposed."}
{"id": "PMID:471510", "title": "Audiometric evaluation of noise induced hearing loss.", "content": "Audiometric evaluation in conjunction with other components of the hearing conservation program serves to minimize the potential hazard of noise related hearing loss. Success of the industrial hearing conservation program rests in large measure with the implementation of competent audiometric testing procedures. Thus, consideration must be given to certain critical factors necessary for measurement of hearing sensitivity, such as the qualifications of the examiner, monitoring schedules, test environment, instrumentation, method of testing, recording of results, interpretation of test findings, and appropriate referral criteria.", "contents": "Audiometric evaluation of noise induced hearing loss. Audiometric evaluation in conjunction with other components of the hearing conservation program serves to minimize the potential hazard of noise related hearing loss. Success of the industrial hearing conservation program rests in large measure with the implementation of competent audiometric testing procedures. Thus, consideration must be given to certain critical factors necessary for measurement of hearing sensitivity, such as the qualifications of the examiner, monitoring schedules, test environment, instrumentation, method of testing, recording of results, interpretation of test findings, and appropriate referral criteria."}
{"id": "PMID:471512", "title": "Aural rehabilitation in noise induced hearing loss.", "content": "The patient with noise induced hearing loss presents complex aural rehabilitative problems. Such patients are not generally good candidates for \"traditional\" aural rehabilitation involving speech reading therapy and auditory training. Instead aural rehabilitation efforts involve individual evaluation of the communication handicap, non-medical relief for tinnitus, possible hearing aid use with carefully selected amplification and earmold, and counseling on hearing and listening.", "contents": "Aural rehabilitation in noise induced hearing loss. The patient with noise induced hearing loss presents complex aural rehabilitative problems. Such patients are not generally good candidates for \"traditional\" aural rehabilitation involving speech reading therapy and auditory training. Instead aural rehabilitation efforts involve individual evaluation of the communication handicap, non-medical relief for tinnitus, possible hearing aid use with carefully selected amplification and earmold, and counseling on hearing and listening."}
{"id": "PMID:471514", "title": "Congenital syphilis.", "content": "The most outstanding aspects of congenital or fetal syphilis, emphasizing its epidemiology, clinical aspects, diagnosis, prevention and treatment, are studied. The authors point out the increasing frequency of this treponematosis, which should still be considered as a worldwide endemic disease, though it is foreseeable and tractable. Some new data about the problem are provided, apropos of recent observations.", "contents": "Congenital syphilis. The most outstanding aspects of congenital or fetal syphilis, emphasizing its epidemiology, clinical aspects, diagnosis, prevention and treatment, are studied. The authors point out the increasing frequency of this treponematosis, which should still be considered as a worldwide endemic disease, though it is foreseeable and tractable. Some new data about the problem are provided, apropos of recent observations."}
{"id": "PMID:471515", "title": "Candida infections.", "content": "The authors present a review of the epidemiology, pathology, diagnosis and treatment of candidiasis in the child. Their studies on the favoring factors in cutaneous forms as well as their experiences in pulmonary forms are emphasized.", "contents": "Candida infections. The authors present a review of the epidemiology, pathology, diagnosis and treatment of candidiasis in the child. Their studies on the favoring factors in cutaneous forms as well as their experiences in pulmonary forms are emphasized."}
{"id": "PMID:471516", "title": "Neonatal sepsis.", "content": "A review of neonatal sepsis, based on 30 recent cases is presented. After analyzing their personal experiences, the authors review the etiopathogeny (higher incidence in small-for-date, preterm, postterm and in some macrosomic newborns; predominance of gram-negative germs), the importance of early diagnosis, and correct treatment, adapting the antibiotherapy to the bacteriological flora of the location at the time of treatment.", "contents": "Neonatal sepsis. A review of neonatal sepsis, based on 30 recent cases is presented. After analyzing their personal experiences, the authors review the etiopathogeny (higher incidence in small-for-date, preterm, postterm and in some macrosomic newborns; predominance of gram-negative germs), the importance of early diagnosis, and correct treatment, adapting the antibiotherapy to the bacteriological flora of the location at the time of treatment."}
{"id": "PMID:471517", "title": "Mycoplasma pneumoniae infections.", "content": "A review of infections with Mycoplasma pneumoniae in children, emphasizing their frequency, association with viral infections, nonspecific clinical aspects, diagnostic data, and extrapulmonary possible manifestations is presented. This work was based on 320 analyses made in a period of 3 years. 15 of those analyses were serologically positive for M. pneumoniae, and 10 of them were also serologically positive for virus.", "contents": "Mycoplasma pneumoniae infections. A review of infections with Mycoplasma pneumoniae in children, emphasizing their frequency, association with viral infections, nonspecific clinical aspects, diagnostic data, and extrapulmonary possible manifestations is presented. This work was based on 320 analyses made in a period of 3 years. 15 of those analyses were serologically positive for M. pneumoniae, and 10 of them were also serologically positive for virus."}
{"id": "PMID:471518", "title": "Current status of active immunization procedures.", "content": "A review of the present status of the principal active immunization procedures used in pediatric ages is presented. Great advances are emphasized in smallpox, diphtheria, tetanus, poliomyelitis, measles, rubella, etc. Others less effective vaccine or those at an experimental stage, are also described. A vaccination calendar is also presented.", "contents": "Current status of active immunization procedures. A review of the present status of the principal active immunization procedures used in pediatric ages is presented. Great advances are emphasized in smallpox, diphtheria, tetanus, poliomyelitis, measles, rubella, etc. Others less effective vaccine or those at an experimental stage, are also described. A vaccination calendar is also presented."}
{"id": "PMID:471519", "title": "Septic shock in pediatrics.", "content": "A review of the septic shock syndrome in pediatrics, analyzing its actual incidence in clinical practice, etiopathological factors, physiopathology, clinical manifestations in the different stages of the disease, diagnostic criteria, prognostic, and treatment is presented.", "contents": "Septic shock in pediatrics. A review of the septic shock syndrome in pediatrics, analyzing its actual incidence in clinical practice, etiopathological factors, physiopathology, clinical manifestations in the different stages of the disease, diagnostic criteria, prognostic, and treatment is presented."}
{"id": "PMID:471521", "title": "[Asplenia and DIC (author's transl)].", "content": "3 cases of severe septic shock are described; a 5 month old girl with congenital hyposplenia, a 2 3/12 year old boy splenectomized because of microspherozytosis and a 11 6/12 year old boy splenectomized because of Hodgkin's disease. In 2 cases pneumococci were found in the blood cultures. In all 3 cases the coagulation analysis showed a consumption coagulopathy. Intravenous streptokinase treatment was applied in addition to general treatment for shock and antibiotic therapy. 2 patients survived and made a complete recovery, whereas the 2 year old boy died. The histological findings showed a severe DIC. In the Department of Surgery, Innsbruck, 44 children have been splenectomized during the last 6 years, 38 of whom we were able to follow up on for an average of 3.3 years. After an average of 1.2 years following splenectomy, 4 patients (including the 3 cases mentioned above) contracted acute septicaemia; a further patient also incurring a probable sepsis with DIC. 3 of these 5 children died, representing a morbidity rate of 13% and a mortality rate of 8%. The mortality rate is thus as high as that caused by the primary disease, indicating the urgency of prophylaxis for infections of this kind. 3 prophylactic forms of treatment are suggested: protection with penicillin, active immunization with polyvalent pneumococcal antigen and spleen preservation whenever possible.", "contents": "[Asplenia and DIC (author's transl)]. 3 cases of severe septic shock are described; a 5 month old girl with congenital hyposplenia, a 2 3/12 year old boy splenectomized because of microspherozytosis and a 11 6/12 year old boy splenectomized because of Hodgkin's disease. In 2 cases pneumococci were found in the blood cultures. In all 3 cases the coagulation analysis showed a consumption coagulopathy. Intravenous streptokinase treatment was applied in addition to general treatment for shock and antibiotic therapy. 2 patients survived and made a complete recovery, whereas the 2 year old boy died. The histological findings showed a severe DIC. In the Department of Surgery, Innsbruck, 44 children have been splenectomized during the last 6 years, 38 of whom we were able to follow up on for an average of 3.3 years. After an average of 1.2 years following splenectomy, 4 patients (including the 3 cases mentioned above) contracted acute septicaemia; a further patient also incurring a probable sepsis with DIC. 3 of these 5 children died, representing a morbidity rate of 13% and a mortality rate of 8%. The mortality rate is thus as high as that caused by the primary disease, indicating the urgency of prophylaxis for infections of this kind. 3 prophylactic forms of treatment are suggested: protection with penicillin, active immunization with polyvalent pneumococcal antigen and spleen preservation whenever possible."}
{"id": "PMID:471522", "title": "[Childhood injuries treated at a university clinic for traumatology (author's transl)].", "content": "The accidents to all children up to the age of 14 years admitted to the I. Universit\u00e4tsklinik f\u00fcr Unfallchirurgie in Wien in 1976 (2977 children) were analysed as to age-distribution, cause of accident, type and location of injury. There was an increase in accidents to older children. 13% of the injuries were due to traffic accidents; 60% occurred in the course of play; 27% on the field of sport. More than 60% were only minor injuries such as abrasions, lacerations, crushed fingers and toes etc., 30% bone fractures and ligamental injuries; 2,5% brain traumas. Most of the bone fractures were localized iment and no lethal injuries.", "contents": "[Childhood injuries treated at a university clinic for traumatology (author's transl)]. The accidents to all children up to the age of 14 years admitted to the I. Universit\u00e4tsklinik f\u00fcr Unfallchirurgie in Wien in 1976 (2977 children) were analysed as to age-distribution, cause of accident, type and location of injury. There was an increase in accidents to older children. 13% of the injuries were due to traffic accidents; 60% occurred in the course of play; 27% on the field of sport. More than 60% were only minor injuries such as abrasions, lacerations, crushed fingers and toes etc., 30% bone fractures and ligamental injuries; 2,5% brain traumas. Most of the bone fractures were localized iment and no lethal injuries."}
{"id": "PMID:471523", "title": "[Exaggerated somatomedin activity in the Beckwith-Wiedemann syndrome (author's transl)].", "content": "Beckwith and Wiedemann described the syndrome of exomphalos, macroglossia and gigantism with hypoglycemia and visceral organ hyperplasias. In some cases of severe hypoglycemias hyperplasia of beta cells of the pancreas was found. Hyperinsulinism, which has to date rarely been investigated, reacts strongly to beta cell stimulation and can hardly be suppressed. The cause of gigantism and organ hyperplasias is still unknown. After a short description of a case of hypoglycemias in the first two weeks of life a long-term profile of the endocrinologic abnormalities and carbohydrate metabolism is given. Growth hormone response to insulin is normal, tolbutamide is followed by severe hypoglycemias without an increase in the immunoreactive insulin levels; the activity of somatomedin is excessively increased. The high activity of somatomedin explains the high potency of growth in the different tissues and the hypoglycemic reactions and it seems reasonable to assume that somatomedin could create nesidioblastosis of the pancreas with hyperinsulinism and severe hypoglycemias. It is likely that the Beckwith-Wiedemann syndrome and the Laron type familial dwarfism with high plasma growth hormone, absent activity of somatomedin, and disorders in carbohydrate metabolism represent complementary diseases.", "contents": "[Exaggerated somatomedin activity in the Beckwith-Wiedemann syndrome (author's transl)]. Beckwith and Wiedemann described the syndrome of exomphalos, macroglossia and gigantism with hypoglycemia and visceral organ hyperplasias. In some cases of severe hypoglycemias hyperplasia of beta cells of the pancreas was found. Hyperinsulinism, which has to date rarely been investigated, reacts strongly to beta cell stimulation and can hardly be suppressed. The cause of gigantism and organ hyperplasias is still unknown. After a short description of a case of hypoglycemias in the first two weeks of life a long-term profile of the endocrinologic abnormalities and carbohydrate metabolism is given. Growth hormone response to insulin is normal, tolbutamide is followed by severe hypoglycemias without an increase in the immunoreactive insulin levels; the activity of somatomedin is excessively increased. The high activity of somatomedin explains the high potency of growth in the different tissues and the hypoglycemic reactions and it seems reasonable to assume that somatomedin could create nesidioblastosis of the pancreas with hyperinsulinism and severe hypoglycemias. It is likely that the Beckwith-Wiedemann syndrome and the Laron type familial dwarfism with high plasma growth hormone, absent activity of somatomedin, and disorders in carbohydrate metabolism represent complementary diseases."}
{"id": "PMID:471524", "title": "[Subclavian steal syndrome in childhood (author's transl)].", "content": "The authors report on four children with subclavian steal associated with coarctation or interrupted aortic arch. All children showed a stenotic origin of the left subclavian; in addition three of them had an extreme hypoplasia of the proximal segment of this artery. Two children had typical symptoms of insufficiency of the vertebral and basilar circulation (headache, dizziness, syncopal attacks, vision and hearing disorders) since the age of 7 and 8 years, respectively. The clinical findings, such as reduced pulses and blood pressure of the respective arm, may suggest the provisional diagnosis. The principles of congenital subclavian steal are discussed.", "contents": "[Subclavian steal syndrome in childhood (author's transl)]. The authors report on four children with subclavian steal associated with coarctation or interrupted aortic arch. All children showed a stenotic origin of the left subclavian; in addition three of them had an extreme hypoplasia of the proximal segment of this artery. Two children had typical symptoms of insufficiency of the vertebral and basilar circulation (headache, dizziness, syncopal attacks, vision and hearing disorders) since the age of 7 and 8 years, respectively. The clinical findings, such as reduced pulses and blood pressure of the respective arm, may suggest the provisional diagnosis. The principles of congenital subclavian steal are discussed."}
{"id": "PMID:471525", "title": "[Tetracyclin intoxication versus idiopathic pancreatitis: report of a case with multiple organ involvement (author's transl)].", "content": "The tetracycline class of antibiotics is infrequently used in clinical pediatrics due to its side effects: they include anorexia, nausea, vomiting and diarrhea. Hypersensitivity, a photosensibility reaction and a brownish discoloration of teeth is less frequently, a pseudotumor cerebri is rarely seen. Once therapeutic plasma levels are exceeded however, either by overdosage or decreased renal or hepatic clearance of the drug, serious complications like a secondary Fanconi-Syndrom or a nephrogenic diabetes insipidus can occur. The increased toxicity of tetracyclines in pregnant women is well known. We would like to report a fatal case, where serious complications like a secondary Fanconi-Syndrom, toxic degeneration of the liver, a clinically undected pancreatitis and a protein loosing enteropathy are though to be either direct consequences of tetracycline overdosage or the indirect effect of a shocklike syndrom by means of a nonoliguric renal failure induced by tetracycline.", "contents": "[Tetracyclin intoxication versus idiopathic pancreatitis: report of a case with multiple organ involvement (author's transl)]. The tetracycline class of antibiotics is infrequently used in clinical pediatrics due to its side effects: they include anorexia, nausea, vomiting and diarrhea. Hypersensitivity, a photosensibility reaction and a brownish discoloration of teeth is less frequently, a pseudotumor cerebri is rarely seen. Once therapeutic plasma levels are exceeded however, either by overdosage or decreased renal or hepatic clearance of the drug, serious complications like a secondary Fanconi-Syndrom or a nephrogenic diabetes insipidus can occur. The increased toxicity of tetracyclines in pregnant women is well known. We would like to report a fatal case, where serious complications like a secondary Fanconi-Syndrom, toxic degeneration of the liver, a clinically undected pancreatitis and a protein loosing enteropathy are though to be either direct consequences of tetracycline overdosage or the indirect effect of a shocklike syndrom by means of a nonoliguric renal failure induced by tetracycline."}
{"id": "PMID:471526", "title": "[Pneumoperitoneum in a newborn without intestinal perforation (author's transl)].", "content": "This is the report of the rare complication of an isolated pneumoperitoneum in a premature infant of 28 weeks gestation with artificial ventilation due to severe RDS. This rare occurrence in immature babies with artificial ventilation should be considered in the differential diagnosis of abdominal emergencies in this age group. Etiology as well as therapeutic consequences will be discussed.", "contents": "[Pneumoperitoneum in a newborn without intestinal perforation (author's transl)]. This is the report of the rare complication of an isolated pneumoperitoneum in a premature infant of 28 weeks gestation with artificial ventilation due to severe RDS. This rare occurrence in immature babies with artificial ventilation should be considered in the differential diagnosis of abdominal emergencies in this age group. Etiology as well as therapeutic consequences will be discussed."}
{"id": "PMID:471527", "title": "[Thrombasthenia in a newborn (author's transl)].", "content": "Thrombasthenia is an extemely rare hereditary disorder of platelet function characterized by impairment of adhesiveness, ADP-induced aggregation, and clot retraction. Petechiae and ecchymoses in a newborn infant followed by prolonged jaundice facilitated early diagnosis.", "contents": "[Thrombasthenia in a newborn (author's transl)]. Thrombasthenia is an extemely rare hereditary disorder of platelet function characterized by impairment of adhesiveness, ADP-induced aggregation, and clot retraction. Petechiae and ecchymoses in a newborn infant followed by prolonged jaundice facilitated early diagnosis."}
{"id": "PMID:471531", "title": "The inheritance of thiabendazole resistance in Haemonchus contortus.", "content": "Haemonchus contortus worm populations isolated from naturally infected sheep at the Pastoral Research Laboratory, Armidale, N.S.W., were found to contain approximately 20% of worms resistant to a 50 mg/kg dose of thiabendazole. Following 3 generations of selection with 50 mg/kg thiabendazole the number of worms removed by the anthelmintic was too small to detect differences between treated and control groups. After more than 15 generations of selection, matings between males from the selected strain and non-resistant females produced resistant males and females in equal numbers. Thus, thiabendazole resistance does not appear to be sex-linked. A dose--response assay on the F2 adults indicated that worms from female resistant x male non-resistant crosses were more resistant than F2 adults of the reciprocal cross. An in vitro technique that identified thiabendazole-resistant eggs by their ability to hatch in a solution containing thiabendazole and 0.1% NaCl solution was also used to study the inheritance of resistance. F1 eggs had similar LC50's to the resistant parents. F2 and back-cross eggs from an original mating of thiabendazole-resistant females x non-resistant males had a higher LC50 than F2 and back-cross eggs from the reciprocal mating, indicating a degree of matroclinous inheritance of resistance. However, the resistant parents had tolerances to thiabendazole exceeding those of F2. F3 eggs had a resistance distribution that ranged from that of the resistant to the non-resistant parent. No significant deviation from linearity was observed in any of the dose--response lines. These results indicate that thiabendazole resistance in H. contortus worms is inherited as an autosomal and semi-dominant trait.", "contents": "The inheritance of thiabendazole resistance in Haemonchus contortus. Haemonchus contortus worm populations isolated from naturally infected sheep at the Pastoral Research Laboratory, Armidale, N.S.W., were found to contain approximately 20% of worms resistant to a 50 mg/kg dose of thiabendazole. Following 3 generations of selection with 50 mg/kg thiabendazole the number of worms removed by the anthelmintic was too small to detect differences between treated and control groups. After more than 15 generations of selection, matings between males from the selected strain and non-resistant females produced resistant males and females in equal numbers. Thus, thiabendazole resistance does not appear to be sex-linked. A dose--response assay on the F2 adults indicated that worms from female resistant x male non-resistant crosses were more resistant than F2 adults of the reciprocal cross. An in vitro technique that identified thiabendazole-resistant eggs by their ability to hatch in a solution containing thiabendazole and 0.1% NaCl solution was also used to study the inheritance of resistance. F1 eggs had similar LC50's to the resistant parents. F2 and back-cross eggs from an original mating of thiabendazole-resistant females x non-resistant males had a higher LC50 than F2 and back-cross eggs from the reciprocal mating, indicating a degree of matroclinous inheritance of resistance. However, the resistant parents had tolerances to thiabendazole exceeding those of F2. F3 eggs had a resistance distribution that ranged from that of the resistant to the non-resistant parent. No significant deviation from linearity was observed in any of the dose--response lines. These results indicate that thiabendazole resistance in H. contortus worms is inherited as an autosomal and semi-dominant trait."}
{"id": "PMID:471532", "title": "Transplantation of adult Trichinella spiralis between hosts: worm survival and immunological characteristics of the host--parasite relationship.", "content": "A technique for the transplantation of Trichinella spiralis worms directly into the host intestine is described. Infections established by the direct transfer of adult worms were essentially normal both in terms of their survival and reproduction and in their stimulation of, and susceptibility to, host immune responses. Worms transplanted from NIH mouse donors at intervals after infection had an equal ability to survive in the recipient, even when taken from the donor shortly before or during the process of worm expulsion, showing that expulsion does not require worms to be irreversibly damaged. It was noted, however, that after 7 days in the donor the ability of the worm to reproduce in the recipient was temporarily impaired.", "contents": "Transplantation of adult Trichinella spiralis between hosts: worm survival and immunological characteristics of the host--parasite relationship. A technique for the transplantation of Trichinella spiralis worms directly into the host intestine is described. Infections established by the direct transfer of adult worms were essentially normal both in terms of their survival and reproduction and in their stimulation of, and susceptibility to, host immune responses. Worms transplanted from NIH mouse donors at intervals after infection had an equal ability to survive in the recipient, even when taken from the donor shortly before or during the process of worm expulsion, showing that expulsion does not require worms to be irreversibly damaged. It was noted, however, that after 7 days in the donor the ability of the worm to reproduce in the recipient was temporarily impaired."}
{"id": "PMID:471533", "title": "Oogenesis and egg-shell formation in Aspiculuris tetraptera Schulz (Nematoda: Oxyuroidea).", "content": "The ovary of Aspiculuris tetraptera has a prominent terminal cap cell. This is considered to be part of the ovarian epithelium. Oogonia detach from the short rachis and increase in size from 6 to 60 microns; accumulating hyaline granules, shell granules and glycogen. The hyaline granules persist in the eff cytoplasm after shell formation has been completed and are considered to be lipoprotein yolk. The shell granules contribute to the non-chitin fraction of the chitinous layer. A classification of the cytoplasmic inclusions of the nematode oocyte is proposed. Upon fertilization a vitelline membrane is formed which constitutes the vitelline layer of the egg-shell. The chitinous layer is secreted in the perivitelline space, between the vitelline layer and the egg oolemma. Upon completion of chitinous layer synthesis, the egg cytoplasm contracts away from its inner surface. The material of the lipid layer is secreted at the surface of the egg cytoplasm and adheres to the inner surface of the chitinous layer. During secretion of the chitinous and lipid layers by the egg cytoplasm, the uterine cells secrete the unit membrane-like external uterine layer and the crystalline internal uterine layer. A complex system of interconnecting spaces develops in the internal uterine layer. This system is open to the exterior via breaks in the external uterine layer. There is no direct involvement of the uterine cells in the formation of this structure.", "contents": "Oogenesis and egg-shell formation in Aspiculuris tetraptera Schulz (Nematoda: Oxyuroidea). The ovary of Aspiculuris tetraptera has a prominent terminal cap cell. This is considered to be part of the ovarian epithelium. Oogonia detach from the short rachis and increase in size from 6 to 60 microns; accumulating hyaline granules, shell granules and glycogen. The hyaline granules persist in the eff cytoplasm after shell formation has been completed and are considered to be lipoprotein yolk. The shell granules contribute to the non-chitin fraction of the chitinous layer. A classification of the cytoplasmic inclusions of the nematode oocyte is proposed. Upon fertilization a vitelline membrane is formed which constitutes the vitelline layer of the egg-shell. The chitinous layer is secreted in the perivitelline space, between the vitelline layer and the egg oolemma. Upon completion of chitinous layer synthesis, the egg cytoplasm contracts away from its inner surface. The material of the lipid layer is secreted at the surface of the egg cytoplasm and adheres to the inner surface of the chitinous layer. During secretion of the chitinous and lipid layers by the egg cytoplasm, the uterine cells secrete the unit membrane-like external uterine layer and the crystalline internal uterine layer. A complex system of interconnecting spaces develops in the internal uterine layer. This system is open to the exterior via breaks in the external uterine layer. There is no direct involvement of the uterine cells in the formation of this structure."}
{"id": "PMID:471534", "title": "The structure of the egg-shell of Aspiculuris tetraptera Schulz (Nematoda: Oxyuroidea).", "content": "The egg of Aspiculuris tetraptera is an ellipsoid measuring 93 x 40 microns. The shell consists of 5 layers: the external uterine layer, internal uterine layer, vitelline layer, chitinous layer and the lipid layer. This nomenclature is based upon the formation and histochemistry of the shell layers. The internal uterine layer contains a system of interconnecting spaces, partly filled by uterine secretion, which open to the exterior of the egg via breaks in the external uterine layer. The surface of the egg is covered by a system of interconnecting grooves. Freeze-etching reveals that the internal uterine layer is open to the exterior via pores, which open into the grooves. Rod-shaped particles are also revealed in the external uterine layer. The operculum of the egg consists of a modification of the uterine and chitinous layers of the shell.", "contents": "The structure of the egg-shell of Aspiculuris tetraptera Schulz (Nematoda: Oxyuroidea). The egg of Aspiculuris tetraptera is an ellipsoid measuring 93 x 40 microns. The shell consists of 5 layers: the external uterine layer, internal uterine layer, vitelline layer, chitinous layer and the lipid layer. This nomenclature is based upon the formation and histochemistry of the shell layers. The internal uterine layer contains a system of interconnecting spaces, partly filled by uterine secretion, which open to the exterior of the egg via breaks in the external uterine layer. The surface of the egg is covered by a system of interconnecting grooves. Freeze-etching reveals that the internal uterine layer is open to the exterior via pores, which open into the grooves. Rod-shaped particles are also revealed in the external uterine layer. The operculum of the egg consists of a modification of the uterine and chitinous layers of the shell."}
{"id": "PMID:471535", "title": "Further observations on immunization of sheep against Schistosoma mansoni and S. bovis using irradiation-attenuated schistosomula of homologous and heterologous species.", "content": "This paper describes further characteristics of the immunization of sheep against schistosomes using live, irradiation schistosomula. Sheep immunized with a non-virulent strain of Schistosoma mattheei were protected against a more virulent strain of the same species for over a year. As there was no evidence that the irradiated parasites were able to persist this long, it was concluded that the vaccine had induced a sterile resistance. Heterologous vaccination, using irradiated S. mattheei schistosomula to immunize against S. bovis or irradiated S. mansoni schistosomula to immunize against S. mattheei, failed to induce any protection.", "contents": "Further observations on immunization of sheep against Schistosoma mansoni and S. bovis using irradiation-attenuated schistosomula of homologous and heterologous species. This paper describes further characteristics of the immunization of sheep against schistosomes using live, irradiation schistosomula. Sheep immunized with a non-virulent strain of Schistosoma mattheei were protected against a more virulent strain of the same species for over a year. As there was no evidence that the irradiated parasites were able to persist this long, it was concluded that the vaccine had induced a sterile resistance. Heterologous vaccination, using irradiated S. mattheei schistosomula to immunize against S. bovis or irradiated S. mansoni schistosomula to immunize against S. mattheei, failed to induce any protection."}
{"id": "PMID:471536", "title": "A reappraisal of the taxonomic status of Eimeria mivati Edgar and Seibold 1964, by enzyme electrophoresis and cross-immunity tests.", "content": "An examination of 2 strains of Eimeria acervulina var. mivati (since 1973 E. mivati has been regarded as a variant of E. acervulina) showed that previous confusion over the taxonomic status of E. mivati arose because the investigations were done using laboratory cultures of E. mivati which were contaiminated with E. acervulina. Electrophoretic analyses of enzymes, host specificity and cross-immunity tests have revealed that: (1) The 1971 Houghton strain of E. acervulina var. mivati was a mixture of 2 parasites. (a) Passage of this strain in embryonating eggs resulted in a selection against that parasite previously characterized as E. acervulina. (b) The parasite which did reproduce in eggs did not immunize chickens against subsequent challenge with E. acervulina. This parasite is most likely E. mivati. (c) E. mivati recovered from eggs did, however, immunize chickens against challenge with a new field strain which was morphologically identical to E. mivati and characterized by the same electrophoretic forms of 2 enzymes. (2) A strain of E. acervulina var. mivati from the USA was also a mixture of E. acervulina and E. mivati.", "contents": "A reappraisal of the taxonomic status of Eimeria mivati Edgar and Seibold 1964, by enzyme electrophoresis and cross-immunity tests. An examination of 2 strains of Eimeria acervulina var. mivati (since 1973 E. mivati has been regarded as a variant of E. acervulina) showed that previous confusion over the taxonomic status of E. mivati arose because the investigations were done using laboratory cultures of E. mivati which were contaiminated with E. acervulina. Electrophoretic analyses of enzymes, host specificity and cross-immunity tests have revealed that: (1) The 1971 Houghton strain of E. acervulina var. mivati was a mixture of 2 parasites. (a) Passage of this strain in embryonating eggs resulted in a selection against that parasite previously characterized as E. acervulina. (b) The parasite which did reproduce in eggs did not immunize chickens against subsequent challenge with E. acervulina. This parasite is most likely E. mivati. (c) E. mivati recovered from eggs did, however, immunize chickens against challenge with a new field strain which was morphologically identical to E. mivati and characterized by the same electrophoretic forms of 2 enzymes. (2) A strain of E. acervulina var. mivati from the USA was also a mixture of E. acervulina and E. mivati."}
{"id": "PMID:471537", "title": "Rejection of Eimeria by foreign hosts.", "content": "Oocysts of Eimeria maxima inoculated into guinea fowl do not develop. Infection occurs when intestinal mucosa or liver tissue from guinea fowl given E. maxima is transferred to susceptible chickens. By transferring material at different times after inoculation, it was shown that most of the stages are lost between 6 and 12 h and few, if any, survive to 48 h. Sporozoites of E. tenella had low infectivity after 48 h contact in vitro with peritoneal macrophages from guinea fowls and turkeys. Sporozoites of E. grenieri from the guinea fowl appeared to be destroyed within macrophages taken from chickens.", "contents": "Rejection of Eimeria by foreign hosts. Oocysts of Eimeria maxima inoculated into guinea fowl do not develop. Infection occurs when intestinal mucosa or liver tissue from guinea fowl given E. maxima is transferred to susceptible chickens. By transferring material at different times after inoculation, it was shown that most of the stages are lost between 6 and 12 h and few, if any, survive to 48 h. Sporozoites of E. tenella had low infectivity after 48 h contact in vitro with peritoneal macrophages from guinea fowls and turkeys. Sporozoites of E. grenieri from the guinea fowl appeared to be destroyed within macrophages taken from chickens."}
{"id": "PMID:471556", "title": "The triad of pneumonitis, pleuritis, and pericarditis in juvenile rheumatoid arthritis.", "content": "There are few reports of pleuroparenchymal and pericardial involvement secondary to juvenile rheumatoid arthritis (JRA) in the literature. This article presents two such cases and gathers scattered information about this combination previously reported by few others. We wish to emphasize that the combination of pericardial and pleuropulmonary involvement in a youngster may be the only presenting clinical manifestations of rheumatoid arthritis, whereas in others, it may be associated with arthritic symptoms at the same time.", "contents": "The triad of pneumonitis, pleuritis, and pericarditis in juvenile rheumatoid arthritis. There are few reports of pleuroparenchymal and pericardial involvement secondary to juvenile rheumatoid arthritis (JRA) in the literature. This article presents two such cases and gathers scattered information about this combination previously reported by few others. We wish to emphasize that the combination of pericardial and pleuropulmonary involvement in a youngster may be the only presenting clinical manifestations of rheumatoid arthritis, whereas in others, it may be associated with arthritic symptoms at the same time."}
{"id": "PMID:471557", "title": "A potential pitfall of operative cholangiography in infants.", "content": "Retrograde linear extravasation of contrast media along the anatomical course of intrahepatic arborizing structures may occur during intraoperative cholangiography for evaluation of prolonged neonatal jaundice. The resultant image may mimic the appearance of intrahepatic biliary radicles.", "contents": "A potential pitfall of operative cholangiography in infants. Retrograde linear extravasation of contrast media along the anatomical course of intrahepatic arborizing structures may occur during intraoperative cholangiography for evaluation of prolonged neonatal jaundice. The resultant image may mimic the appearance of intrahepatic biliary radicles."}
{"id": "PMID:471558", "title": "Ultrasound diagnosis of extrahepatic portal vein obstruction in childhood. The sign of the subhepatic sponge-like mass.", "content": "Portal vein obstruction in childhood produces portal hypertension and hepatopetal collaterals surrounding the obstructed portal vein; this is the \"cavernomatous transformation of the portal vein\" which is a well defined angiographic entity. Clinically findings are the various manifestations of Banti's syndrome, especially splenomegaly and gastrointestinal bleeding. Ultrasonic diagnosis is possible on the associated findings of splenomegaly, normal liver, a subhepatic sponge like mass corresponding to the hepatopetal collaterals. These signs must be sought in the ultrasonic differential diagnosis of splenomegaly in pediatrics.", "contents": "Ultrasound diagnosis of extrahepatic portal vein obstruction in childhood. The sign of the subhepatic sponge-like mass. Portal vein obstruction in childhood produces portal hypertension and hepatopetal collaterals surrounding the obstructed portal vein; this is the \"cavernomatous transformation of the portal vein\" which is a well defined angiographic entity. Clinically findings are the various manifestations of Banti's syndrome, especially splenomegaly and gastrointestinal bleeding. Ultrasonic diagnosis is possible on the associated findings of splenomegaly, normal liver, a subhepatic sponge like mass corresponding to the hepatopetal collaterals. These signs must be sought in the ultrasonic differential diagnosis of splenomegaly in pediatrics."}
{"id": "PMID:471559", "title": "Coexistent neuroblastoma and Hirschsprung's disease--another manifestation of the neurocristopathy?", "content": "Abnormalities of neural crest development may be manifested in a wide variety of ways ranging from von Recklinghausen's disease to Sipple's syndrome -- pheochromocytoma and medullary thyroid carcinoma. We report here yet another manifestation -- coexistent multiple neuroblastomas and Hirschsprung's disease in a newborn. In a review of the English literature we have found the combination of neuroblastoma and Hirschsprung's disease recorded only once previously [2]. However, the relationship between the two diseases was not discussed. The two diseases may be at the extremes of a spectrum of neural crest development pathology, i.e., Hirschsprung's representing a deficiency and neuroblastoma representing malignant proliferation of cells of neural crest origin.", "contents": "Coexistent neuroblastoma and Hirschsprung's disease--another manifestation of the neurocristopathy? Abnormalities of neural crest development may be manifested in a wide variety of ways ranging from von Recklinghausen's disease to Sipple's syndrome -- pheochromocytoma and medullary thyroid carcinoma. We report here yet another manifestation -- coexistent multiple neuroblastomas and Hirschsprung's disease in a newborn. In a review of the English literature we have found the combination of neuroblastoma and Hirschsprung's disease recorded only once previously [2]. However, the relationship between the two diseases was not discussed. The two diseases may be at the extremes of a spectrum of neural crest development pathology, i.e., Hirschsprung's representing a deficiency and neuroblastoma representing malignant proliferation of cells of neural crest origin."}
{"id": "PMID:471561", "title": "Radiation gonad doses received by children in intravenous urography and micturition cysto-urethrography.", "content": "Measurements of gonad doses in intravenous urography (IVU) was performed on 47 children and in micturition cysto-urethrography (MCU) on 57 children. Ionization chamber dosimeters were placed rectally in girls and fixed on the scrotum in boys. Gonad dose ranged from 27 mrad to 201 mrad in girls and from 7 mrad to 112 mrad in boys during IVU; during MCU doses ranged from 53 mrad to 1900 mrad in girls and form 24 mrad to 309 mrad in boys. Risk of leukemia and genetic risk were calculated.", "contents": "Radiation gonad doses received by children in intravenous urography and micturition cysto-urethrography. Measurements of gonad doses in intravenous urography (IVU) was performed on 47 children and in micturition cysto-urethrography (MCU) on 57 children. Ionization chamber dosimeters were placed rectally in girls and fixed on the scrotum in boys. Gonad dose ranged from 27 mrad to 201 mrad in girls and from 7 mrad to 112 mrad in boys during IVU; during MCU doses ranged from 53 mrad to 1900 mrad in girls and form 24 mrad to 309 mrad in boys. Risk of leukemia and genetic risk were calculated."}
{"id": "PMID:471562", "title": "Sclerosteosis in children.", "content": "Craniotubular dysplasias and craniotubular hyperostoses are a group of confusing disorders in which the cranial involvement may cause compression of facial and auditory nerves. Two of the hyperostotic conditions that regularly produce cranial nerve compression are sclerosteosis and the recessive form of endosteal hyperostosis (van Buchem's disease). Both have identical radiological features with cranial involvement presenting in childhood. The Dutch and South African cases have come from relatively close-knit communities, those in the latter country being Afrikaners who had their origins in Holland. The radiological features of three such cases of sclerosteosis are briefly presented.", "contents": "Sclerosteosis in children. Craniotubular dysplasias and craniotubular hyperostoses are a group of confusing disorders in which the cranial involvement may cause compression of facial and auditory nerves. Two of the hyperostotic conditions that regularly produce cranial nerve compression are sclerosteosis and the recessive form of endosteal hyperostosis (van Buchem's disease). Both have identical radiological features with cranial involvement presenting in childhood. The Dutch and South African cases have come from relatively close-knit communities, those in the latter country being Afrikaners who had their origins in Holland. The radiological features of three such cases of sclerosteosis are briefly presented."}
{"id": "PMID:471563", "title": "A familial syndrome of diaphyseal cortical thickening of the long bones, bowed legs, tendency to fracture and icthyosis.", "content": "The clinical and radiological features of a dominant bone disorder found in six members of the same family are described. The bone disorder was mainly characterized by endosteal cortical thickening of long tubular bones and bowing of the weight-bearing ones. The clinical symptoms were waddling gait, muscle weakness and leg pains, and there was a tendency to fractures. All affected individuals had icthyosis of slight or moderate degree.", "contents": "A familial syndrome of diaphyseal cortical thickening of the long bones, bowed legs, tendency to fracture and icthyosis. The clinical and radiological features of a dominant bone disorder found in six members of the same family are described. The bone disorder was mainly characterized by endosteal cortical thickening of long tubular bones and bowing of the weight-bearing ones. The clinical symptoms were waddling gait, muscle weakness and leg pains, and there was a tendency to fractures. All affected individuals had icthyosis of slight or moderate degree."}
{"id": "PMID:471564", "title": "Haemorrhagic right upper lobe infarction as a complication in primary end-to-end anastomosis of oesophageal atresia.", "content": "The clinical and radiological features of right upper lobe haemorrhagic infarction as a complication to end-to-end anastomosis in oesophageal atresia are presented.", "contents": "Haemorrhagic right upper lobe infarction as a complication in primary end-to-end anastomosis of oesophageal atresia. The clinical and radiological features of right upper lobe haemorrhagic infarction as a complication to end-to-end anastomosis in oesophageal atresia are presented."}
{"id": "PMID:471565", "title": "Early osseous abnormalities in Menkes' kinky hair syndrome.", "content": "Bone changes in a 4 m.o. infant with Menkes' Kinky Hair Syndrome, who also had a skeletal survey as a newborn, are reported. Whereas the late bone changes were diagnostic, the neonatal ones were similar to congenital rubella and cytomegalic virus infection. However the distribution of the metaphyseal changes were different.", "contents": "Early osseous abnormalities in Menkes' kinky hair syndrome. Bone changes in a 4 m.o. infant with Menkes' Kinky Hair Syndrome, who also had a skeletal survey as a newborn, are reported. Whereas the late bone changes were diagnostic, the neonatal ones were similar to congenital rubella and cytomegalic virus infection. However the distribution of the metaphyseal changes were different."}
{"id": "PMID:471566", "title": "Common pulmonary vein atresia without anomalous pulmonary venous connection.", "content": "Common pulmonary vein atresia without pulmonary venous connection is a rare form of congenital heart disease. No communication exists between the confluence of the pulmonary veins and the heart or a major systemic vessel. A case diagnosed antemortem is presented. Correct early diagnosis is imperative as surgery may be corrective.", "contents": "Common pulmonary vein atresia without anomalous pulmonary venous connection. Common pulmonary vein atresia without pulmonary venous connection is a rare form of congenital heart disease. No communication exists between the confluence of the pulmonary veins and the heart or a major systemic vessel. A case diagnosed antemortem is presented. Correct early diagnosis is imperative as surgery may be corrective."}
{"id": "PMID:471567", "title": "Rheologic properties of erythrocytes in Duchenne muscular dystrophy.", "content": "The transformation of erythrocytes into echinocytes by suspension in isotonic phosphate buffer was increased in patients with Duchenne muscular dystrophy (DMD) as compared to healthy controls. The transformation was enhanced when glass slides instead of polystyrole slides were used for observation. Viscosity of erythrocyte suspensions with a hematocrit of 80% was the same in patients and controls. The mean flow rate of erythrocyte suspensions in autologous plasma was only slightly reduced in the patient group, but decreased markedly after suspension of the cells in phosphate buffer, without as well as after preincubation with 10 mM Na salicylate. The decreased flow rate in patients is probably due to the increased number of echinocytes and not to an increased membrane rigidity, because the membrane flexibility of isolated ghosts, measured in a viscosimeter, was normal at all shear rates. Echinocyte formation in patients with DMD is strongly dependent on the techniques applied and difficult to evaluate quantitatively by phase contrast microscopy. Filtration of erythrocyte suspensions seems to be a more reliable, quantitative test for increased erythrocyte vulnerability.", "contents": "Rheologic properties of erythrocytes in Duchenne muscular dystrophy. The transformation of erythrocytes into echinocytes by suspension in isotonic phosphate buffer was increased in patients with Duchenne muscular dystrophy (DMD) as compared to healthy controls. The transformation was enhanced when glass slides instead of polystyrole slides were used for observation. Viscosity of erythrocyte suspensions with a hematocrit of 80% was the same in patients and controls. The mean flow rate of erythrocyte suspensions in autologous plasma was only slightly reduced in the patient group, but decreased markedly after suspension of the cells in phosphate buffer, without as well as after preincubation with 10 mM Na salicylate. The decreased flow rate in patients is probably due to the increased number of echinocytes and not to an increased membrane rigidity, because the membrane flexibility of isolated ghosts, measured in a viscosimeter, was normal at all shear rates. Echinocyte formation in patients with DMD is strongly dependent on the techniques applied and difficult to evaluate quantitatively by phase contrast microscopy. Filtration of erythrocyte suspensions seems to be a more reliable, quantitative test for increased erythrocyte vulnerability."}
{"id": "PMID:471568", "title": "Some observations on the properties of precipitating antibody to Staphylococcus aureus in children with kwashiorkor.", "content": "Using three relatively sensitive immunochemical assay procedures, we were unable to detect either precipitating or hemagglutinating antibodies to food antigens in sera from normal and kwashiorkor Nigerian children. However, antibody to Staphylococcus aureus, a common pathogenic bacteria in the tropics, could be detected in the sera of 28 of 30 control children but in only 7 of 30 kwashiorkor cases, suggesting that there is an impairment to specific antibody production in children with malnutrition. Comparative biologic and physicochemical studies carried out on precipitating antibodies to S. aureus isolated from normal and kwashiorkor subjects showed that the antibody from malnourished individuals fixed complement less readily and was deficient in some essential amino acids. These defects might in part explain why children with protein-energy malnutrition are more susceptible to infection in spite of the fact that they have normal serum immunoglobulin concentrations.", "contents": "Some observations on the properties of precipitating antibody to Staphylococcus aureus in children with kwashiorkor. Using three relatively sensitive immunochemical assay procedures, we were unable to detect either precipitating or hemagglutinating antibodies to food antigens in sera from normal and kwashiorkor Nigerian children. However, antibody to Staphylococcus aureus, a common pathogenic bacteria in the tropics, could be detected in the sera of 28 of 30 control children but in only 7 of 30 kwashiorkor cases, suggesting that there is an impairment to specific antibody production in children with malnutrition. Comparative biologic and physicochemical studies carried out on precipitating antibodies to S. aureus isolated from normal and kwashiorkor subjects showed that the antibody from malnourished individuals fixed complement less readily and was deficient in some essential amino acids. These defects might in part explain why children with protein-energy malnutrition are more susceptible to infection in spite of the fact that they have normal serum immunoglobulin concentrations."}
{"id": "PMID:471570", "title": "Perinatal changes of 2,3-diphosphoglycerate and oxygen affinity in mammals not having fetal type hemoglobins.", "content": "The interrelationship of 2,3-diphosphoglycerate (2,3-DPG) and P50 levels during the fetal and postnatal life were determined in two mammalian species which do not have a switchover of hemoglobin type at the end of their fetal development. In the guinea pig and rabbit, the 2,3-DPG levels remain low during fetal life and increase only after birth remaining elevated throughout adult life. The adult levels were reached at 2 days of age in the guinea pig and the 20th day in rabbit. Fetal P50 values increased only after birth, paralleling the rise in the 2,3-DPG. The rapidity of the postnatal rise in 2,3-DPG and decrease in P50 appears related to the maturity of the newborn animal at birth in these species.", "contents": "Perinatal changes of 2,3-diphosphoglycerate and oxygen affinity in mammals not having fetal type hemoglobins. The interrelationship of 2,3-diphosphoglycerate (2,3-DPG) and P50 levels during the fetal and postnatal life were determined in two mammalian species which do not have a switchover of hemoglobin type at the end of their fetal development. In the guinea pig and rabbit, the 2,3-DPG levels remain low during fetal life and increase only after birth remaining elevated throughout adult life. The adult levels were reached at 2 days of age in the guinea pig and the 20th day in rabbit. Fetal P50 values increased only after birth, paralleling the rise in the 2,3-DPG. The rapidity of the postnatal rise in 2,3-DPG and decrease in P50 appears related to the maturity of the newborn animal at birth in these species."}
{"id": "PMID:471571", "title": "Equations for estimating percentage of body fat in children 10--14 years old.", "content": "Measurements of the percentage of body fat (derived by underwater weighing), height, weight, and triceps skinfold thickness were determined for a volunteer sample of 214 children, 10--14 years old. The bivariate correlations, by sex, of percentage of body fat with five obesity indices are highest with the triceps skinfold and least with weight-height. Five sets of equations are presented for predicting percentage of body fat based on measurements of either height and weight, triceps skinfold, or height, weight, and triceps skinfold. Cumulative frequency distributions of the percentage of body fat (based on height, weight, and triceps skinfold) are shown by race and sex for a total community of 10- to 14-year olds.", "contents": "Equations for estimating percentage of body fat in children 10--14 years old. Measurements of the percentage of body fat (derived by underwater weighing), height, weight, and triceps skinfold thickness were determined for a volunteer sample of 214 children, 10--14 years old. The bivariate correlations, by sex, of percentage of body fat with five obesity indices are highest with the triceps skinfold and least with weight-height. Five sets of equations are presented for predicting percentage of body fat based on measurements of either height and weight, triceps skinfold, or height, weight, and triceps skinfold. Cumulative frequency distributions of the percentage of body fat (based on height, weight, and triceps skinfold) are shown by race and sex for a total community of 10- to 14-year olds."}
{"id": "PMID:471572", "title": "Cerebral blood flow during acute acidosis in perinatal goats.", "content": "Changes in cerebral blood flow in response to three states of acute acidosis, posthypoxic, lactic acid, and respiratory, were estimated by the microsphere technique. In all three states, the fraction of the systemic blood flow reaching the brain and the rate (ml/min) of blood flow to it increased. The increase in flow was linearly related both to the PaCO2 and to H+. Others have shown the flow rate to increase with hypercapnia, but the increase associated with an increase in hydrogen ion concentration while the PaCO2 was maintained at control levels does not appear to have been observed in mature animals.", "contents": "Cerebral blood flow during acute acidosis in perinatal goats. Changes in cerebral blood flow in response to three states of acute acidosis, posthypoxic, lactic acid, and respiratory, were estimated by the microsphere technique. In all three states, the fraction of the systemic blood flow reaching the brain and the rate (ml/min) of blood flow to it increased. The increase in flow was linearly related both to the PaCO2 and to H+. Others have shown the flow rate to increase with hypercapnia, but the increase associated with an increase in hydrogen ion concentration while the PaCO2 was maintained at control levels does not appear to have been observed in mature animals."}
{"id": "PMID:471573", "title": "Immunoglobulin subclasses in normal children.", "content": "The range, mean, and normal bounds of serum IgG 1--4 subclass concentrations in children 0--16 years old are given. Levels increase with age, plateauing at different ages. Definition of the normal range will permit better identification of individuals with either low or high levels.", "contents": "Immunoglobulin subclasses in normal children. The range, mean, and normal bounds of serum IgG 1--4 subclass concentrations in children 0--16 years old are given. Levels increase with age, plateauing at different ages. Definition of the normal range will permit better identification of individuals with either low or high levels."}
{"id": "PMID:471574", "title": "Infant heart rate response to trigeminal airstream stimulation: determination of normal and deviant values.", "content": "In this study, trigeminal airstream stimulation is used on a group of normal, sleeping infants to screen for reflex bradycardia. Infants were tested at 1--3 days and at 4, 8, 12, and 16 weeks of age. The usual heart rate response was acceleration above the prestimulus level. The amount of heart rate change was seen to depend on prestimulus rate and, to a lesser extent, postconceptional age. Multiple regression analysis of the data provided estimates of expected responses, given postconceptional age and prestimulus heart rate. From these, ranges were established for classification of normal or abnormal responses. Six deviant data points over 2 SDs from expected values were identified. Two of these represented heart rate increases and four were heart rate decreases. One deviant heart rate decrease was over 3 SDs from the mean. Although the deviant increases were thought to represent exaggerated startles, the more severe heart rate decreases were believed to be a reflex resembling the diving reflex.", "contents": "Infant heart rate response to trigeminal airstream stimulation: determination of normal and deviant values. In this study, trigeminal airstream stimulation is used on a group of normal, sleeping infants to screen for reflex bradycardia. Infants were tested at 1--3 days and at 4, 8, 12, and 16 weeks of age. The usual heart rate response was acceleration above the prestimulus level. The amount of heart rate change was seen to depend on prestimulus rate and, to a lesser extent, postconceptional age. Multiple regression analysis of the data provided estimates of expected responses, given postconceptional age and prestimulus heart rate. From these, ranges were established for classification of normal or abnormal responses. Six deviant data points over 2 SDs from expected values were identified. Two of these represented heart rate increases and four were heart rate decreases. One deviant heart rate decrease was over 3 SDs from the mean. Although the deviant increases were thought to represent exaggerated startles, the more severe heart rate decreases were believed to be a reflex resembling the diving reflex."}
{"id": "PMID:471575", "title": "Hormone ontogeny in the ovine fetus. IV. Serum somatomedin activity in the fetal and neonatal lamb and pregnant ewe: correlation with maternal and fetal growth hormone, prolactin, and chorionic somatomammotropin.", "content": "Somatomedin (SM) activity was measured by a placental membrane receptor assay using 125I-labeled somatomedin A, as radioligand in serum samples obtained from 33 ovine fetuses, 14 neonatal lambs, 8 pregnant, and 3 postpartum ewes. The mean serum concentration of SM activity in eight adult rams was 2.06 +/- 0.12 U/ml. In fetal sheep, SM activity was detected at 66 days gestation (term 147 days), in the youngest fetus studied. Before 100 days of gestation, SM was lower (P less than 0.001) in fetal sheep (1.08 +/- 0.18 U/ml) than in adult rams. In fetuses between 101 and 125 days, SM rose (P less than 0.001) to 2.64 +/- 0.32 U/ml. In late gestation fetal serum SM fell but during the neonatal period it rose to 3.38 +/- 0.3 U/ml, higher (P less than 0.01) than that in adult rams. Serum SM activity in the pregnant ewe prior to 100 days was 1.01 +/- 0.11 U/ml, increased (P less than 0.05) to 1.75 +/- 0.21 U/ml between 125 days and term, and rose further to 2.56 +/- 0.32 U/ml in the postpartum period. Maternal concentrations of serum SM in late gestation were significantly less than in the fetus. Gel chromatography of fetal, maternal, and neonatal sera indicated that over 90% of SM activity circulated in high-molecular weight form. The rise in SM activity in fetal serum between 100 and 125 days parallels the rise in fetal growth hormone and prolactin concentrations; however, in maternal serum the increase in SM activity is associated with rising maternal chorionic somatomammotropin concentrations.", "contents": "Hormone ontogeny in the ovine fetus. IV. Serum somatomedin activity in the fetal and neonatal lamb and pregnant ewe: correlation with maternal and fetal growth hormone, prolactin, and chorionic somatomammotropin. Somatomedin (SM) activity was measured by a placental membrane receptor assay using 125I-labeled somatomedin A, as radioligand in serum samples obtained from 33 ovine fetuses, 14 neonatal lambs, 8 pregnant, and 3 postpartum ewes. The mean serum concentration of SM activity in eight adult rams was 2.06 +/- 0.12 U/ml. In fetal sheep, SM activity was detected at 66 days gestation (term 147 days), in the youngest fetus studied. Before 100 days of gestation, SM was lower (P less than 0.001) in fetal sheep (1.08 +/- 0.18 U/ml) than in adult rams. In fetuses between 101 and 125 days, SM rose (P less than 0.001) to 2.64 +/- 0.32 U/ml. In late gestation fetal serum SM fell but during the neonatal period it rose to 3.38 +/- 0.3 U/ml, higher (P less than 0.01) than that in adult rams. Serum SM activity in the pregnant ewe prior to 100 days was 1.01 +/- 0.11 U/ml, increased (P less than 0.05) to 1.75 +/- 0.21 U/ml between 125 days and term, and rose further to 2.56 +/- 0.32 U/ml in the postpartum period. Maternal concentrations of serum SM in late gestation were significantly less than in the fetus. Gel chromatography of fetal, maternal, and neonatal sera indicated that over 90% of SM activity circulated in high-molecular weight form. The rise in SM activity in fetal serum between 100 and 125 days parallels the rise in fetal growth hormone and prolactin concentrations; however, in maternal serum the increase in SM activity is associated with rising maternal chorionic somatomammotropin concentrations."}
{"id": "PMID:471576", "title": "Inducibility of metallothionein biosynthesis in cultured normal and Menkes kinky hair disease fibroblasts: effects of copper and cadmium.", "content": "Metallothionein biosynthesis is not induced by extracellular copper in Menkes Kinky hair disease (MKHD) or in normal cultured fibroblasts under the conditions of these experiments. In the presence of copper, MKHD fibroblasts also incorporated less cysteine than did normal fibroblasts. Extracellular cadmium greatly enhanced the uptake of cysteine in both normal and MKHD cultures. By the technique of polyacrylamide gel electrophoresis, it was demonstrated that metallothionein is induced by cadmium in normal and MKHD-cultured fibroblasts.", "contents": "Inducibility of metallothionein biosynthesis in cultured normal and Menkes kinky hair disease fibroblasts: effects of copper and cadmium. Metallothionein biosynthesis is not induced by extracellular copper in Menkes Kinky hair disease (MKHD) or in normal cultured fibroblasts under the conditions of these experiments. In the presence of copper, MKHD fibroblasts also incorporated less cysteine than did normal fibroblasts. Extracellular cadmium greatly enhanced the uptake of cysteine in both normal and MKHD cultures. By the technique of polyacrylamide gel electrophoresis, it was demonstrated that metallothionein is induced by cadmium in normal and MKHD-cultured fibroblasts."}
{"id": "PMID:471579", "title": "Energy retention, energy expenditure, and growth in healthy immature infants.", "content": "Energy balance studies were done during 10-29 days on 15 immature infants of mean birth weight 1581 g. Mean gross energy intake was 757 kJ/kg (181 kcal) and 79% of this was retained, so that metabolizable energy was 602 kJ/kg (143 kcal). Mean resting metabolic rate was 244 kJ/kg (58.1 kcal), and it increased with advancing maturity. Minimum resting metabolism averaged 199 kJ/kg (47.5 kcal). Energy expended in activity increased with maturity, but amounted to less than 17% of the total energy turnover. Postprandial metabolism caused the mean VO2 to rise by 17% in the hour after a feed, and during 24 hr resulted in consumption of energy equivalent to about 10% of the resting metabolism. Stored energy amounted to 230 kJ/kg (55 kcal) and was linearly related to weight gain (r = 0.92). Energy cost of weight gain was 24 kJ/g (5.7 kcal) and energy stored in new tissue was 16.8 kJ/g (4.0 kcal). Maintenance energy requirement at zero growth rate was about 270 kJ/kg (64 kcal).", "contents": "Energy retention, energy expenditure, and growth in healthy immature infants. Energy balance studies were done during 10-29 days on 15 immature infants of mean birth weight 1581 g. Mean gross energy intake was 757 kJ/kg (181 kcal) and 79% of this was retained, so that metabolizable energy was 602 kJ/kg (143 kcal). Mean resting metabolic rate was 244 kJ/kg (58.1 kcal), and it increased with advancing maturity. Minimum resting metabolism averaged 199 kJ/kg (47.5 kcal). Energy expended in activity increased with maturity, but amounted to less than 17% of the total energy turnover. Postprandial metabolism caused the mean VO2 to rise by 17% in the hour after a feed, and during 24 hr resulted in consumption of energy equivalent to about 10% of the resting metabolism. Stored energy amounted to 230 kJ/kg (55 kcal) and was linearly related to weight gain (r = 0.92). Energy cost of weight gain was 24 kJ/g (5.7 kcal) and energy stored in new tissue was 16.8 kJ/g (4.0 kcal). Maintenance energy requirement at zero growth rate was about 270 kJ/kg (64 kcal)."}
{"id": "PMID:471580", "title": "Transient partial hGH deficiency in prepubertal children with delay of growth.", "content": "The hGH response to an ornithin or an insulin test was measured in 105 children from 11-18 yr old with delay of growth more than 2 SD. Besides 74 subjects with normal values and 7 with complete lack of response, 24 subjects exhibited a partial rise of GH. Most of the latter had decreasing growth rate and no sign of puberty. Out of 15 assessed for GH function after onset of puberty, 14 showed a normal response accompanying a markedly increased growth velocity. Four other subjects with partial response who were receiving sexual hormones for 48 hr before the second test showed a normal response also. An exogenous hGH treatment administered in two of these patients resulted in a marked and sustained growth increase far before entering puberty. The findings that plasma GH responses to stimulation tests is reduced in some children with decreasing growth rate before puberty and return to normal after onset of sexual development, would suggest a transient and functional defect in growth hormone secretion and the physiologic role of sexual hormones on GH release mechanism at that period of life.", "contents": "Transient partial hGH deficiency in prepubertal children with delay of growth. The hGH response to an ornithin or an insulin test was measured in 105 children from 11-18 yr old with delay of growth more than 2 SD. Besides 74 subjects with normal values and 7 with complete lack of response, 24 subjects exhibited a partial rise of GH. Most of the latter had decreasing growth rate and no sign of puberty. Out of 15 assessed for GH function after onset of puberty, 14 showed a normal response accompanying a markedly increased growth velocity. Four other subjects with partial response who were receiving sexual hormones for 48 hr before the second test showed a normal response also. An exogenous hGH treatment administered in two of these patients resulted in a marked and sustained growth increase far before entering puberty. The findings that plasma GH responses to stimulation tests is reduced in some children with decreasing growth rate before puberty and return to normal after onset of sexual development, would suggest a transient and functional defect in growth hormone secretion and the physiologic role of sexual hormones on GH release mechanism at that period of life."}
{"id": "PMID:471583", "title": "Adaptive gluconeogenesis in preterm and term rabbits.", "content": "Carbohydrate metabolism in the developing rabbit was investigated for deficiencies that may be responsible for the failure of many preterm (28 1/2--29 1/2  day) animals to survive the first hours of life. The preterm animal shows an inability to reverse glycogenolysis or initiate gluconeogenesis from lactate or alanine in the first hours of life. This impairment, coupled with 50% less liver glycogen stores than the term animal, places the preterm animal at jeopardy for energy substrate early on in life. Unexpected was the early, rapid conversion of glycerol to glucose by the preterm animal. This ability seemed to be the primary difference in carbohydrate metabolism between the surviving and nonsurviving preterm rabbit. Impaired glyconeogenesis from lactate and alanine in the preterm animal coupled with active gluconeogenesis from glycerol suggests that substrates from lipolysis may be very important for early adaptation. Preterm animals endowed with limited fat stores, thus, minimal available glycerol, would be incapable of survival.", "contents": "Adaptive gluconeogenesis in preterm and term rabbits. Carbohydrate metabolism in the developing rabbit was investigated for deficiencies that may be responsible for the failure of many preterm (28 1/2--29 1/2  day) animals to survive the first hours of life. The preterm animal shows an inability to reverse glycogenolysis or initiate gluconeogenesis from lactate or alanine in the first hours of life. This impairment, coupled with 50% less liver glycogen stores than the term animal, places the preterm animal at jeopardy for energy substrate early on in life. Unexpected was the early, rapid conversion of glycerol to glucose by the preterm animal. This ability seemed to be the primary difference in carbohydrate metabolism between the surviving and nonsurviving preterm rabbit. Impaired glyconeogenesis from lactate and alanine in the preterm animal coupled with active gluconeogenesis from glycerol suggests that substrates from lipolysis may be very important for early adaptation. Preterm animals endowed with limited fat stores, thus, minimal available glycerol, would be incapable of survival."}
{"id": "PMID:471585", "title": "Lung volume changes, occlusion pressure and chest wall configuration in human infants.", "content": "Measurements of respiration were compared in normal, full term and premature infants before and after application of 5 cm H2O continuous negative pressure (CNEG) around the body below the neck. Mean minute ventilation in the full term infants decreased when CNEG was applied, secondary to a decrease in respiratory rate, with tidal volume relatively unchanged. The premature infants showed no consistent ventilatory response to CNEG. There were variable changes in occlusion pressure at increased lung volume. Observation with rib cage and abdomen anterior-posterior (A-P) magnetometers showed that the increase in end-expiratory lung volume was accounted for largely by expansion of the rib cage so that the configuration of the diaphragm and its mechanical advantage were maintained. These results suggest that the diaphragm is the major determinant of mask occlusion pressure in infants. The effects of increased lung volume on the configuration of the chest wall may relate to the success of continuous distending pressure in the treatment of respiratory disorders in infants, including apnea of prematurity.", "contents": "Lung volume changes, occlusion pressure and chest wall configuration in human infants. Measurements of respiration were compared in normal, full term and premature infants before and after application of 5 cm H2O continuous negative pressure (CNEG) around the body below the neck. Mean minute ventilation in the full term infants decreased when CNEG was applied, secondary to a decrease in respiratory rate, with tidal volume relatively unchanged. The premature infants showed no consistent ventilatory response to CNEG. There were variable changes in occlusion pressure at increased lung volume. Observation with rib cage and abdomen anterior-posterior (A-P) magnetometers showed that the increase in end-expiratory lung volume was accounted for largely by expansion of the rib cage so that the configuration of the diaphragm and its mechanical advantage were maintained. These results suggest that the diaphragm is the major determinant of mask occlusion pressure in infants. The effects of increased lung volume on the configuration of the chest wall may relate to the success of continuous distending pressure in the treatment of respiratory disorders in infants, including apnea of prematurity."}
{"id": "PMID:471586", "title": "Control of vasopressin secretion in the newborn lamb.", "content": "The plasma sodium, osmolality, and arginine vasopressin (AVP) responses to phlebotomy, hypertonic saline, water loading and fluid restriction were studied in 2--49 day old lambs. Phlebotomy of 10 and 20% of the lamb's estimated blood volume produced 37- and 44-fold increments in plasma AVP, without a concomitant change in plasma sodium or osmolality. The infusion of 10 mEq/kg sodium chloride produced a 12% rise in plasma sodium concentration accompanied by a 7-fold rise in plasma AVP. Water loading with 100 ml/kg hypotonic fluid produced a significant fall in plasma sodium concentration (10.7%) and a decrease in plasma AVP. Eighteen hr of water deprivation evoked a 7-fold increase in AVP. These results indicate that the newborn lamb is capable of responding appropriately to known stimuli for AVP secretion. The stimulus response ratio (SRR): (formula: see text) of newborn lambs was nearly identical after hypertonic saline and water loading and was also quite similar to that of the adult ewe after a saline challenge. The SRR of water deprived lambs was greater than that after the other stimuli, presumably reflecting combined volume and osmolar stimuli. We conclude that the neurohypophysis and the volume receptor systems of the newborn lamb are capable of appropriate, mature AVP responsiveness during the first days of extrauterine life.", "contents": "Control of vasopressin secretion in the newborn lamb. The plasma sodium, osmolality, and arginine vasopressin (AVP) responses to phlebotomy, hypertonic saline, water loading and fluid restriction were studied in 2--49 day old lambs. Phlebotomy of 10 and 20% of the lamb's estimated blood volume produced 37- and 44-fold increments in plasma AVP, without a concomitant change in plasma sodium or osmolality. The infusion of 10 mEq/kg sodium chloride produced a 12% rise in plasma sodium concentration accompanied by a 7-fold rise in plasma AVP. Water loading with 100 ml/kg hypotonic fluid produced a significant fall in plasma sodium concentration (10.7%) and a decrease in plasma AVP. Eighteen hr of water deprivation evoked a 7-fold increase in AVP. These results indicate that the newborn lamb is capable of responding appropriately to known stimuli for AVP secretion. The stimulus response ratio (SRR): (formula: see text) of newborn lambs was nearly identical after hypertonic saline and water loading and was also quite similar to that of the adult ewe after a saline challenge. The SRR of water deprived lambs was greater than that after the other stimuli, presumably reflecting combined volume and osmolar stimuli. We conclude that the neurohypophysis and the volume receptor systems of the newborn lamb are capable of appropriate, mature AVP responsiveness during the first days of extrauterine life."}
{"id": "PMID:471587", "title": "Prolonged expiratory duration with elevated lung volume in newborn infants.", "content": "End-expiratory lung volume increased 17.5 +/- 5.4 ml (mean +/- SD) in full term infants and 7.7 +/- 2.1 ml in premature infants when 5--7 cm H2O continuous negative pressure (CNEG) was applied around the thorax. In the full term infants, respiratory rate decreased from 52--43 min-1 (P less than 0.001), mean inspiratory duration (ti) was unchanged, and mean duration of expiration (te) increased from 0.62 +/- 0.14 (SE)-0.84 +/- 0.22 sec (P less than 0.001) after application of CNEG. Te of occluded efforts on CNEG was also prolonged (P less than 0.005), although less than te of spontaneous breaths on CNEG (P less than 0.005). Te increased in four of eight premature infants when CNEG was applied. We conclude that phasic vagal feedback regulates Ti and te is controlled by tonic vagal activity. Premature infants may have an optimum functional residual capacity (FRC) which can be achieved with continuous distending pressure, resulting in regularization of respiration.", "contents": "Prolonged expiratory duration with elevated lung volume in newborn infants. End-expiratory lung volume increased 17.5 +/- 5.4 ml (mean +/- SD) in full term infants and 7.7 +/- 2.1 ml in premature infants when 5--7 cm H2O continuous negative pressure (CNEG) was applied around the thorax. In the full term infants, respiratory rate decreased from 52--43 min-1 (P less than 0.001), mean inspiratory duration (ti) was unchanged, and mean duration of expiration (te) increased from 0.62 +/- 0.14 (SE)-0.84 +/- 0.22 sec (P less than 0.001) after application of CNEG. Te of occluded efforts on CNEG was also prolonged (P less than 0.005), although less than te of spontaneous breaths on CNEG (P less than 0.005). Te increased in four of eight premature infants when CNEG was applied. We conclude that phasic vagal feedback regulates Ti and te is controlled by tonic vagal activity. Premature infants may have an optimum functional residual capacity (FRC) which can be achieved with continuous distending pressure, resulting in regularization of respiration."}
{"id": "PMID:471588", "title": "Potassium: aldosterone relationships in pregnant ewes and chronically cannulated ovine fetuses.", "content": "As maternal hyperkalemia quickly induces fetal hyperkalemia, it was decided to investigate the effect of small elevations of plasma [K+] on the peripheral blood aldosterone concentration in chronically catheterized ovine fetuses and to compare this response to that obtained in the adult ewe both pregnant and nonpregnant. Fol elevations of plasma [K+] of 0.1 to 0.3 mmole/liter blood aldosterone concentrations were elevated by 6.7 +/- 4.9 (9) ng/dl in nonpregnant sheep, 6.3 +/- 5.0 (6) in pregnant ewes, but only 0.3 +/- 1.7 (4) in fetal sheep. For increments in plasma [K+] of 0.4--1.0 mmole/liter aldosterone increments in nonpregnant ewes were 9.5 +/- 4.5 (4) ng/dl compared with 10.1 +/- 4.7 (5) in pregnant ewes and 1.5 +/- 2.6 (7) in fetuses. There was no obvious correlation between the age of the fetus and the response to increased plasma [K+]. In effect, the blood aldosterone concentration of the fetus was not elevated by increasing plasma plasma [K+]. The ovine fetus is not able to cope with hyperkalemia by increasing blood aldosterone concentration. Until the fetus is close to term, hyperkalemia may jeopardize the pregnancy by causing placental hemorrhages.", "contents": "Potassium: aldosterone relationships in pregnant ewes and chronically cannulated ovine fetuses. As maternal hyperkalemia quickly induces fetal hyperkalemia, it was decided to investigate the effect of small elevations of plasma [K+] on the peripheral blood aldosterone concentration in chronically catheterized ovine fetuses and to compare this response to that obtained in the adult ewe both pregnant and nonpregnant. Fol elevations of plasma [K+] of 0.1 to 0.3 mmole/liter blood aldosterone concentrations were elevated by 6.7 +/- 4.9 (9) ng/dl in nonpregnant sheep, 6.3 +/- 5.0 (6) in pregnant ewes, but only 0.3 +/- 1.7 (4) in fetal sheep. For increments in plasma [K+] of 0.4--1.0 mmole/liter aldosterone increments in nonpregnant ewes were 9.5 +/- 4.5 (4) ng/dl compared with 10.1 +/- 4.7 (5) in pregnant ewes and 1.5 +/- 2.6 (7) in fetuses. There was no obvious correlation between the age of the fetus and the response to increased plasma [K+]. In effect, the blood aldosterone concentration of the fetus was not elevated by increasing plasma plasma [K+]. The ovine fetus is not able to cope with hyperkalemia by increasing blood aldosterone concentration. Until the fetus is close to term, hyperkalemia may jeopardize the pregnancy by causing placental hemorrhages."}
{"id": "PMID:471589", "title": "Long-term hemodialysis and nerve conduction in children.", "content": "Initial observations in adults revealed that peripheral neuropathy, as documented by reduced conduction velocity is common in chronic renal failure. Critical analysis of this problem in children on long-term dialysis is scarce, consisting of a simgle report which demonstrated that the motor nerve conduction velocities were decreased early and frequently with more severe depression in peroneal nerve velocities. This is in distinct contrast to data from adults, in whom uniform rates of deterioration are encountered. In addition, a direct correlation of the degree of nerve conduction defect with the severity of the renal failure is found in adult patients. The present study showed a relative lack of nerve conduction defects in 11 children on long-term hemodialysis. With rare exceptions, the conduction velocities were normal. To date, no clinical symptoms of neuropathy were evident in our patients. It would seem that, with the short-dialysis schedule of 12--14 h/wk over a period of up to 5 yr, there is no progressive neuropathy as quantitated by nerve conduction measurements.", "contents": "Long-term hemodialysis and nerve conduction in children. Initial observations in adults revealed that peripheral neuropathy, as documented by reduced conduction velocity is common in chronic renal failure. Critical analysis of this problem in children on long-term dialysis is scarce, consisting of a simgle report which demonstrated that the motor nerve conduction velocities were decreased early and frequently with more severe depression in peroneal nerve velocities. This is in distinct contrast to data from adults, in whom uniform rates of deterioration are encountered. In addition, a direct correlation of the degree of nerve conduction defect with the severity of the renal failure is found in adult patients. The present study showed a relative lack of nerve conduction defects in 11 children on long-term hemodialysis. With rare exceptions, the conduction velocities were normal. To date, no clinical symptoms of neuropathy were evident in our patients. It would seem that, with the short-dialysis schedule of 12--14 h/wk over a period of up to 5 yr, there is no progressive neuropathy as quantitated by nerve conduction measurements."}
{"id": "PMID:471591", "title": "Fetal lamb lung lavage and its effect on lung phosphatidylcholine.", "content": "The lungs of intrauterine 135--136-day-old lambs were lavaged with amnionic fluid, with or without meconium, to determine the effect on lung phosphatidylcholine (PC) concentration, synthesis, and function. No differences were apparent between animals lavaged with amnionic fluid or amnionic fluid with meconium. When lavaged lungs were compared to nonlavaged controls, no detectable differences were observed in histology or the quantity of saturated (SPC) and unsaturated phosphatidylcholine (UPC). However, the lavaged lungs retained a larger fraction of maximal lung volume at 5 cm H2O distending pressure and the incorporation of (32P) orthophosphate into lung PC was significantly reduced. In addition, two lavaged animals who became acidotic (pH less than 7.20) exhibited decreased incorporation of 14C) palmitate into whole lung unsaturated, and saturated phosphatidylcholine. These data indicate that amnionic fluid can reduce lamb lung de novo synthesis of PC and may contribute to the alterations in lung PC found in neonatal syndromes of respiratory distress. Acidosis may accentuate this effect.", "contents": "Fetal lamb lung lavage and its effect on lung phosphatidylcholine. The lungs of intrauterine 135--136-day-old lambs were lavaged with amnionic fluid, with or without meconium, to determine the effect on lung phosphatidylcholine (PC) concentration, synthesis, and function. No differences were apparent between animals lavaged with amnionic fluid or amnionic fluid with meconium. When lavaged lungs were compared to nonlavaged controls, no detectable differences were observed in histology or the quantity of saturated (SPC) and unsaturated phosphatidylcholine (UPC). However, the lavaged lungs retained a larger fraction of maximal lung volume at 5 cm H2O distending pressure and the incorporation of (32P) orthophosphate into lung PC was significantly reduced. In addition, two lavaged animals who became acidotic (pH less than 7.20) exhibited decreased incorporation of 14C) palmitate into whole lung unsaturated, and saturated phosphatidylcholine. These data indicate that amnionic fluid can reduce lamb lung de novo synthesis of PC and may contribute to the alterations in lung PC found in neonatal syndromes of respiratory distress. Acidosis may accentuate this effect."}
{"id": "PMID:471592", "title": "The effects of angiotensin II blockade and nephrectomy on the renin-angiotensin-aldosterone system in the newborn lamb.", "content": "Six newborn lambs were studied during continuous infusion of saralasin acetate, 5 micrograms/kg/min for 135 min; 40 min after beginning saralasin, furosemide (2 mg/kg) was injected over 1--2 min. In addition, six anephric lambs were studied after injection of furosemide. Plasma renin activity (PRA) increases from 23 +/- 2.7 ng/ml/hr (M and SE) to 85.8 +/- 16.5 (P less than 0.05) during infusion of saralasin alone and remained at this level after injection of furosemide. PRA did not increase above base line after injection of furosemide in the anephric lambs. Blood pressure dropped after saralasin infusion in the normal lambs (P less than 0.05), and after furosemide injection (P less than 0.05) in both groups. Plasma aldosterone concentrations did not increase in response to furosemide in either group. The results suggest that angiotensin II is important in maintaining blood pressure in the newborn and exerts antagonistic effects on the renal renin secretion mechanism. The renin-angiotensin-aldosterone system is activated in the normal newborn. Possible reasons for the activation include decreased circumferential tension of small arteries and salt wasting.", "contents": "The effects of angiotensin II blockade and nephrectomy on the renin-angiotensin-aldosterone system in the newborn lamb. Six newborn lambs were studied during continuous infusion of saralasin acetate, 5 micrograms/kg/min for 135 min; 40 min after beginning saralasin, furosemide (2 mg/kg) was injected over 1--2 min. In addition, six anephric lambs were studied after injection of furosemide. Plasma renin activity (PRA) increases from 23 +/- 2.7 ng/ml/hr (M and SE) to 85.8 +/- 16.5 (P less than 0.05) during infusion of saralasin alone and remained at this level after injection of furosemide. PRA did not increase above base line after injection of furosemide in the anephric lambs. Blood pressure dropped after saralasin infusion in the normal lambs (P less than 0.05), and after furosemide injection (P less than 0.05) in both groups. Plasma aldosterone concentrations did not increase in response to furosemide in either group. The results suggest that angiotensin II is important in maintaining blood pressure in the newborn and exerts antagonistic effects on the renal renin secretion mechanism. The renin-angiotensin-aldosterone system is activated in the normal newborn. Possible reasons for the activation include decreased circumferential tension of small arteries and salt wasting."}
{"id": "PMID:471594", "title": "Neonatal mononuclear cell metabolism: further evidence for diminished monocyte function in the neonate.", "content": "The metabolic capacity of neonatal monocytes was compared to the metabolic capacity of adult monocytes by two entirely different methods: the selective diminution of monocyte contamination of whole mononuclear cells and the isolation of relatively purified populations of monocytes. Monocyte removal from whole mononuclear cells produced a diminution in the pyruvate kinase (PK) activity (from 28.6 +/- 1.1 to 15.6 +/- 1.2 nmoles/min/10(7) cells) and an increase in adenosine triphosphate (ATP) content (from 7.9 +/- 1.0 to 9.5 +/- 0.8 nmoles/10(7) cells) in adult cells. No change in PK activity (from 13.5 +/- 1.3 to 14.0 +/- 1.3) was observed in cord cells, but the ATP content of cord cells was higher after monocytes depletion (from 4.7 +/- 0.5 to 6.2 +- 0.7). The suggestion of metabolic vulnerability was confirmed by metabolic analysis of isolated adult and cord monocytes. The PK activity of adult monocytes was greater than that of cord monocytes (57 +/- 9 and 25 +/- 0.3, respectively) and the ATP content of adult monocytes (5.7 +/- 0.2) was greater than that of cord monocytes (2.3 +/- 0.1). The data confirm prior observations of diminished energy metabolism in neonatal mononucler cells and suggest that the metabolic perturbations may, in part, correlate with functional immaturity of the neonatal monocyte.", "contents": "Neonatal mononuclear cell metabolism: further evidence for diminished monocyte function in the neonate. The metabolic capacity of neonatal monocytes was compared to the metabolic capacity of adult monocytes by two entirely different methods: the selective diminution of monocyte contamination of whole mononuclear cells and the isolation of relatively purified populations of monocytes. Monocyte removal from whole mononuclear cells produced a diminution in the pyruvate kinase (PK) activity (from 28.6 +/- 1.1 to 15.6 +/- 1.2 nmoles/min/10(7) cells) and an increase in adenosine triphosphate (ATP) content (from 7.9 +/- 1.0 to 9.5 +/- 0.8 nmoles/10(7) cells) in adult cells. No change in PK activity (from 13.5 +/- 1.3 to 14.0 +/- 1.3) was observed in cord cells, but the ATP content of cord cells was higher after monocytes depletion (from 4.7 +/- 0.5 to 6.2 +- 0.7). The suggestion of metabolic vulnerability was confirmed by metabolic analysis of isolated adult and cord monocytes. The PK activity of adult monocytes was greater than that of cord monocytes (57 +/- 9 and 25 +/- 0.3, respectively) and the ATP content of adult monocytes (5.7 +/- 0.2) was greater than that of cord monocytes (2.3 +/- 0.1). The data confirm prior observations of diminished energy metabolism in neonatal mononucler cells and suggest that the metabolic perturbations may, in part, correlate with functional immaturity of the neonatal monocyte."}
{"id": "PMID:471595", "title": "The labeling of lung phosphatidylcholine in premature rabbits.", "content": "Lung phosphatidylcholine metabolism was studied in vivo in premature rabbits delivered by cesarean section as early in gestation as compatible with prolonged viability (28.9 days). The newborn rabbits initially required supplemental oxygen and had respiratory distress. The amount of phosphatidylcholine isolated from the lung parenchyma changed little over the first 3 days of life, while phosphatidylcholine in the alveolar wash increased in 3 days from 0.05--3.1 mumol/50 g animal. The phosphatidylcholine of the lungs of the premature rabbits was pulse labeled with isotopically labeled palmitic acid, choline, and phosphate given to the pregnant does 10 min before delivery of the newborns. After the initial incorporation period, the total amount of radioactive precursor palmitic acid, choline, or 32P) incorporated into lung phosphatidylcholine did not change for a period of 4 days. Labeled phosphatidylcholine was detected initially in alveolar wash 3 hr after administration of the three precursors and continued to accumulate for many hours. The biological half-life values for lung and alveolar phosphatidylcholine indicated that phosphatidylcholine was turning over very slowly. However, if the effect of dilution on the measured specific activity caused by phosphatidylcholine accumulation was considered, virtually no labeled alveolar or lung phosphatidylcholine disappeared during the 3--4 days of these observations. These results with premature newborn rabbits were similar to those for term newborn rabbits, but different from similar measurements made in the adult rabbit.", "contents": "The labeling of lung phosphatidylcholine in premature rabbits. Lung phosphatidylcholine metabolism was studied in vivo in premature rabbits delivered by cesarean section as early in gestation as compatible with prolonged viability (28.9 days). The newborn rabbits initially required supplemental oxygen and had respiratory distress. The amount of phosphatidylcholine isolated from the lung parenchyma changed little over the first 3 days of life, while phosphatidylcholine in the alveolar wash increased in 3 days from 0.05--3.1 mumol/50 g animal. The phosphatidylcholine of the lungs of the premature rabbits was pulse labeled with isotopically labeled palmitic acid, choline, and phosphate given to the pregnant does 10 min before delivery of the newborns. After the initial incorporation period, the total amount of radioactive precursor palmitic acid, choline, or 32P) incorporated into lung phosphatidylcholine did not change for a period of 4 days. Labeled phosphatidylcholine was detected initially in alveolar wash 3 hr after administration of the three precursors and continued to accumulate for many hours. The biological half-life values for lung and alveolar phosphatidylcholine indicated that phosphatidylcholine was turning over very slowly. However, if the effect of dilution on the measured specific activity caused by phosphatidylcholine accumulation was considered, virtually no labeled alveolar or lung phosphatidylcholine disappeared during the 3--4 days of these observations. These results with premature newborn rabbits were similar to those for term newborn rabbits, but different from similar measurements made in the adult rabbit."}
{"id": "PMID:471596", "title": "Alternative pathway of complement activation in full term and premature infants.", "content": "Classical and alternative pathway complement levels were measured in the cord blood sera of 60 newly born infants, with weights ranging from 1200--4165 g. The impact of maternal illness and infant illness on the complement levels was also evaluated. The mean values for CH50, C3, C4, PH50, factor B, and properdin were all significantly less than normal adult levels (P less than 0.0001). All of the above determinations were significantly correlated with one another except for the relationship between properdin and factor B. CH50, PH50, C4, and properdin levels were significantly correlated with birth weight although there was much residual scatter. Neither maternal illness nor mild to moderate illness in the newborn altered the birth weight-complement relationships. Severe infant illness did significantly alter the relationship between birth weight and complement. However, the impact of this variable on the birth weight-complement relationships was not consistent among the various components. These inconsistencies and the small sample size preclude drawing any strong conclusions about severe illness and complement levels.", "contents": "Alternative pathway of complement activation in full term and premature infants. Classical and alternative pathway complement levels were measured in the cord blood sera of 60 newly born infants, with weights ranging from 1200--4165 g. The impact of maternal illness and infant illness on the complement levels was also evaluated. The mean values for CH50, C3, C4, PH50, factor B, and properdin were all significantly less than normal adult levels (P less than 0.0001). All of the above determinations were significantly correlated with one another except for the relationship between properdin and factor B. CH50, PH50, C4, and properdin levels were significantly correlated with birth weight although there was much residual scatter. Neither maternal illness nor mild to moderate illness in the newborn altered the birth weight-complement relationships. Severe infant illness did significantly alter the relationship between birth weight and complement. However, the impact of this variable on the birth weight-complement relationships was not consistent among the various components. These inconsistencies and the small sample size preclude drawing any strong conclusions about severe illness and complement levels."}
{"id": "PMID:471597", "title": "Purine and phosphoribosylpyrophosphate metabolism of lymphocytes and erythrocytes of an adenosine deaminase deficient immunocompetent child.", "content": "Purine metabolism and phosphoribosylpyrophosphate content of lymphocytes and erythrocytes were studied in an immunocompetent black male child with a total deficiency of erythrocyte and partial deficiency of lymphocyte adenosine deaminase. The partial genetic deficiency of adenosine deaminase was demonstrated in intact lymphocytes, and was approximately one third of the deaminating activity of control lymphocytes. Intact lymphocytes of the patient did not incorporate adenosine at a faster rate than those of control lymphocytes. The patient's erythrocytes deaminating activity was low and adenine ribonucleotide synthesis from adenosine was increased several fold, while adenine incorporation into purine ribonucleotides was comparable to that of control erythrocytes. Transfusion with packed erythrocytes temporarily improved the deaminating capacity of circulating erythrocytes, but did not reduce the elevated incorporation of adenosine into purine ribonucleotides. Phosphoribosylpyrophosphate content of the patient's lymphocytes and erythrocytes was not diminished. Incubation of erythrocytes with adenosine lowered phosphoribosylpyrophosphate content while incubation with phosphate increased phosphoribosylpyrophosphate content to the same extent in mutant and control erythrocytes.", "contents": "Purine and phosphoribosylpyrophosphate metabolism of lymphocytes and erythrocytes of an adenosine deaminase deficient immunocompetent child. Purine metabolism and phosphoribosylpyrophosphate content of lymphocytes and erythrocytes were studied in an immunocompetent black male child with a total deficiency of erythrocyte and partial deficiency of lymphocyte adenosine deaminase. The partial genetic deficiency of adenosine deaminase was demonstrated in intact lymphocytes, and was approximately one third of the deaminating activity of control lymphocytes. Intact lymphocytes of the patient did not incorporate adenosine at a faster rate than those of control lymphocytes. The patient's erythrocytes deaminating activity was low and adenine ribonucleotide synthesis from adenosine was increased several fold, while adenine incorporation into purine ribonucleotides was comparable to that of control erythrocytes. Transfusion with packed erythrocytes temporarily improved the deaminating capacity of circulating erythrocytes, but did not reduce the elevated incorporation of adenosine into purine ribonucleotides. Phosphoribosylpyrophosphate content of the patient's lymphocytes and erythrocytes was not diminished. Incubation of erythrocytes with adenosine lowered phosphoribosylpyrophosphate content while incubation with phosphate increased phosphoribosylpyrophosphate content to the same extent in mutant and control erythrocytes."}
{"id": "PMID:471604", "title": "Prospective study of maternal and infantile infection with Chlamydia trachomatis.", "content": "We examined the prevalence of chlamydial infection in a population of pregnant women and observed their infants to determine the risk of development of ocular or respiratory infection. We examined endocervical and serum specimens from 322 pregnant women for Chlamydia trachomatis and chlamydial antibody. The cultures were obtained at the first prenatal visit. Six (2%) of the women were infected with C trachomatis. Chlamydial antibody was present in the genital secretions of 47% and 73% of the serum samples. The six infants born to infected women, 61 infants born to women who were culture-negative, but local antibody-positive, and 28 control infants born to culture-negative, antibody-negative women were followed for up to six months. Four of six infants born to infected women developed chlamydial infection: two developed culture-positive conjunctivitis, one had asymptomatic nasopharyngeal infection, and one infant developed pneumonitis. Three of 61 infants born to mothers who were culture-negative and local antibody-positive developed conjunctivitis due to C trachomatis. None of the 28 control infants developed chlamydial infection. Most (79%) of the infants had chamydial antibody in their serum at 2 to 4 weeks of age.  The correlation between maternal and infant serum antibody titer was r=0.71 suggesting that antibody was placentally transferred.", "contents": "Prospective study of maternal and infantile infection with Chlamydia trachomatis. We examined the prevalence of chlamydial infection in a population of pregnant women and observed their infants to determine the risk of development of ocular or respiratory infection. We examined endocervical and serum specimens from 322 pregnant women for Chlamydia trachomatis and chlamydial antibody. The cultures were obtained at the first prenatal visit. Six (2%) of the women were infected with C trachomatis. Chlamydial antibody was present in the genital secretions of 47% and 73% of the serum samples. The six infants born to infected women, 61 infants born to women who were culture-negative, but local antibody-positive, and 28 control infants born to culture-negative, antibody-negative women were followed for up to six months. Four of six infants born to infected women developed chlamydial infection: two developed culture-positive conjunctivitis, one had asymptomatic nasopharyngeal infection, and one infant developed pneumonitis. Three of 61 infants born to mothers who were culture-negative and local antibody-positive developed conjunctivitis due to C trachomatis. None of the 28 control infants developed chlamydial infection. Most (79%) of the infants had chamydial antibody in their serum at 2 to 4 weeks of age.  The correlation between maternal and infant serum antibody titer was r=0.71 suggesting that antibody was placentally transferred."}
{"id": "PMID:471606", "title": "Persistent localized pulmonary interstitial emphysema and lymphangiectasia: a causal relationship?", "content": "The mechanism responsible for persistent regional pulmonary interstitial emphysema under tension, which occasionally complicates assisted ventilation, has not been explained. It is difficult to comprehend how interstitial air may cause lobar hyperinflation and not escape to the mediastinum or pleura. Histologic examination of resected emphysematous lung in two neonates with this type of interstitial emphysema revealed air dissecting from the interstitial connective tissue into markedly dilated lymphatics. It is proposed that intralymphatic air dissection causing emphysematous lymphangiectasia may be responsible for fixation of the lung in the emphysematous state.", "contents": "Persistent localized pulmonary interstitial emphysema and lymphangiectasia: a causal relationship? The mechanism responsible for persistent regional pulmonary interstitial emphysema under tension, which occasionally complicates assisted ventilation, has not been explained. It is difficult to comprehend how interstitial air may cause lobar hyperinflation and not escape to the mediastinum or pleura. Histologic examination of resected emphysematous lung in two neonates with this type of interstitial emphysema revealed air dissecting from the interstitial connective tissue into markedly dilated lymphatics. It is proposed that intralymphatic air dissection causing emphysematous lymphangiectasia may be responsible for fixation of the lung in the emphysematous state."}
{"id": "PMID:471607", "title": "Familial spontaneous pneumothorax.", "content": "A family is described in which four persons in three generations suffered spontaneous pneumothoraces: a newborn, an infant, an adolescent, and an adult. Review of the literature reveals 61 reports of familial spontaneous pneumothorax in 22 families. The ratio of male to female cases is approximately 1.8. Affected parents and affected children (including affected fathers and sons) are seen in ten families, while affected siblings with unaffected parents are noted in 13 families. Consanguinity has not been reported. Although autosomal dominant inheritance has been suggested as an explanation of familial spontaneous pneumothorax, available pedigree data are not adequate for statistical analysis. Physicians should be aware of the familial occurrence of spontaneous pneumothorax so that members of such families may be appropriately managed when problems arise.", "contents": "Familial spontaneous pneumothorax. A family is described in which four persons in three generations suffered spontaneous pneumothoraces: a newborn, an infant, an adolescent, and an adult. Review of the literature reveals 61 reports of familial spontaneous pneumothorax in 22 families. The ratio of male to female cases is approximately 1.8. Affected parents and affected children (including affected fathers and sons) are seen in ten families, while affected siblings with unaffected parents are noted in 13 families. Consanguinity has not been reported. Although autosomal dominant inheritance has been suggested as an explanation of familial spontaneous pneumothorax, available pedigree data are not adequate for statistical analysis. Physicians should be aware of the familial occurrence of spontaneous pneumothorax so that members of such families may be appropriately managed when problems arise."}
{"id": "PMID:471609", "title": "Occult fractures in the production of gait disturbance in childhood.", "content": "Trauma to the lower extremities is the principal cause of gait disturbance in early childhood. Three cases are presented to emphasize the relative frequency of children hospitalized for diagnostic evaluation of altered gait who have occult fractures. The cases may refresh the primary physician of the variables that serve as obstacles to accurate diagnosis.", "contents": "Occult fractures in the production of gait disturbance in childhood. Trauma to the lower extremities is the principal cause of gait disturbance in early childhood. Three cases are presented to emphasize the relative frequency of children hospitalized for diagnostic evaluation of altered gait who have occult fractures. The cases may refresh the primary physician of the variables that serve as obstacles to accurate diagnosis."}
{"id": "PMID:471611", "title": "Host defense in infantile osteopetrosis.", "content": "Since infants with malignant osteopetrosis often die from infection at an early age, we studied several aspects of host defense in five such infants. No consistent abnormality was found in cellular or humoral immunity. Monocyte cellular chemotaxis and phagocytosis were normal in four tested infants. However, all four of these infants had decreased intracellular bacterial killing by monocytes. Neutrophil function tests in five infants showed that two had defective bacterial phagocytosis and four had reduced cellular chemotaxis, decreased nitroblue tetrazolium reduction, and decreased intracellular bacterial killing. The severity of the decreased bactericidal capacity of granulocytes did not correlate with the number of circulating immature granulocytes. Our data suggest that abnormal function of circulating monocytes and granulocytes may contribute to impaired host resistance to infection. We postulate that this defect may reflect a more generalized inherited abnormality of phagocytic cells and perhaps osteoclasts that plays a role in the pathogenesis of infantile osteopetrosis.", "contents": "Host defense in infantile osteopetrosis. Since infants with malignant osteopetrosis often die from infection at an early age, we studied several aspects of host defense in five such infants. No consistent abnormality was found in cellular or humoral immunity. Monocyte cellular chemotaxis and phagocytosis were normal in four tested infants. However, all four of these infants had decreased intracellular bacterial killing by monocytes. Neutrophil function tests in five infants showed that two had defective bacterial phagocytosis and four had reduced cellular chemotaxis, decreased nitroblue tetrazolium reduction, and decreased intracellular bacterial killing. The severity of the decreased bactericidal capacity of granulocytes did not correlate with the number of circulating immature granulocytes. Our data suggest that abnormal function of circulating monocytes and granulocytes may contribute to impaired host resistance to infection. We postulate that this defect may reflect a more generalized inherited abnormality of phagocytic cells and perhaps osteoclasts that plays a role in the pathogenesis of infantile osteopetrosis."}
{"id": "PMID:471612", "title": "Neonatal signs as predictors of cerebral palsy.", "content": "Signs of neonatal neurologic dysfunction, recorded in approximately 40,000 infants, were evaluated prospectively for their ability to predict later motor handicap. Tenfold to 33-fold increases in risk of cerebral palsy (CP) were observed in surviving children with any one of the following characteristics: birth weight less than 2,000 gm, head circumference more than 3 SD above or below the mean, five minute Apgar score of 3 or less, diminished activity or diminished cry lasting for more than one day, thermal instability, need for gavage feeding, hypotonia or hypertonia, single or multiple apneic episodes, or hematocrit less than 40%. Of worse portent, with relative risks exceeding 50, were neonatal seizures or Apgar scores of 3 or less at ten minutes or later. These characteristics were also markers of considerable risk of early death. For 0.5% of surviving infants, an overall impression of abnormality of brain function during the nursery period was recorded by the attending physician; there was a 99-fold increase in CP among these children.", "contents": "Neonatal signs as predictors of cerebral palsy. Signs of neonatal neurologic dysfunction, recorded in approximately 40,000 infants, were evaluated prospectively for their ability to predict later motor handicap. Tenfold to 33-fold increases in risk of cerebral palsy (CP) were observed in surviving children with any one of the following characteristics: birth weight less than 2,000 gm, head circumference more than 3 SD above or below the mean, five minute Apgar score of 3 or less, diminished activity or diminished cry lasting for more than one day, thermal instability, need for gavage feeding, hypotonia or hypertonia, single or multiple apneic episodes, or hematocrit less than 40%. Of worse portent, with relative risks exceeding 50, were neonatal seizures or Apgar scores of 3 or less at ten minutes or later. These characteristics were also markers of considerable risk of early death. For 0.5% of surviving infants, an overall impression of abnormality of brain function during the nursery period was recorded by the attending physician; there was a 99-fold increase in CP among these children."}
{"id": "PMID:471613", "title": "Symptomatic arteriovenous malformation in infants less than 6 months of age.", "content": "The symptomatology and sequelae of symptomatic system arteriovenous (A-V) malformations in children less than 6 months old make an early diagnosis possible and early treatment mandatory. A group of 156 infants with systemic A-V malformations involving the CNS (81 infants), liver (61 infants), and lungs (14 infants) were studied to compare symptoms, diagnosis, treatment, and outcome. Congestive heart failure was present in 67% of the patients with CNS A-V malformations. Mortality among the patients with CNS A-V fistulas was 64%. Of those patients with hepatic A-V malformations 67% had abdominal masses, 48% congestive heart failure, and 55% died. Pulmonary A-V malformations presented with congestive heart failure 57%, cyanosis 43%, and resulted in a 43% mortality. Early combined medical and surgical therapy offered the best chance for a successful outcome.", "contents": "Symptomatic arteriovenous malformation in infants less than 6 months of age. The symptomatology and sequelae of symptomatic system arteriovenous (A-V) malformations in children less than 6 months old make an early diagnosis possible and early treatment mandatory. A group of 156 infants with systemic A-V malformations involving the CNS (81 infants), liver (61 infants), and lungs (14 infants) were studied to compare symptoms, diagnosis, treatment, and outcome. Congestive heart failure was present in 67% of the patients with CNS A-V malformations. Mortality among the patients with CNS A-V fistulas was 64%. Of those patients with hepatic A-V malformations 67% had abdominal masses, 48% congestive heart failure, and 55% died. Pulmonary A-V malformations presented with congestive heart failure 57%, cyanosis 43%, and resulted in a 43% mortality. Early combined medical and surgical therapy offered the best chance for a successful outcome."}
{"id": "PMID:471614", "title": "Neonatal intraventricular hemorrhage due to an intracranial arteriovenous malformation: a case report.", "content": "A full-term newborn infant with intraventricular hemorrhage due to a small arteriovenous malformation draining into the vein of Galen is presented. Other reported series are reviewed, and differences between this case and the usual pattern of morbidity are discussed. The use of computerized tomography is demonstrated.", "contents": "Neonatal intraventricular hemorrhage due to an intracranial arteriovenous malformation: a case report. A full-term newborn infant with intraventricular hemorrhage due to a small arteriovenous malformation draining into the vein of Galen is presented. Other reported series are reviewed, and differences between this case and the usual pattern of morbidity are discussed. The use of computerized tomography is demonstrated."}
{"id": "PMID:471615", "title": "Patterns of skin injury in nonaccidental and accidental injury.", "content": "The patterns of soft tissue injuries in a group of 154 children reported to protective services for child abuse or neglect were compared with patterns of soft tissue injury found in a group of 91 emergency room patients who sustained an accidental injury and 105 children seen in a pediatric ambulatory clinic. All children in the three groups were 1 to 12 years old. Children in the suspected child abuse and neglect group had significantly more (P less than .01) soft tissue injuries over the cheeks, trunk, genitals, and upper legs. Lacerations were significantly more common (P less than .001) in the emergency room group.", "contents": "Patterns of skin injury in nonaccidental and accidental injury. The patterns of soft tissue injuries in a group of 154 children reported to protective services for child abuse or neglect were compared with patterns of soft tissue injury found in a group of 91 emergency room patients who sustained an accidental injury and 105 children seen in a pediatric ambulatory clinic. All children in the three groups were 1 to 12 years old. Children in the suspected child abuse and neglect group had significantly more (P less than .01) soft tissue injuries over the cheeks, trunk, genitals, and upper legs. Lacerations were significantly more common (P less than .001) in the emergency room group."}
{"id": "PMID:471616", "title": "Theophylline pharmacokinetics in the young infant.", "content": "The pharmacokinetics of theophylline were investigated in 13 infants, 4 to 18 months of age. An inverse relationship was found between theophylline half-life and age. Volume of distribution did not differ from that reported by other authors in similarly aged infants. Our data suggest that childhood clearance rates of elimination can be achieved by 6 months of age. The decreased theophylline elimination observed in the smaller infant indicates that the usual pediatric dosing recommendations cannot be used routinely. Until more specific data are available in the infant under 6 months, the authors reaffirm individualization of theophylline dosage to maintain therapeutic levels and avoid toxicity.", "contents": "Theophylline pharmacokinetics in the young infant. The pharmacokinetics of theophylline were investigated in 13 infants, 4 to 18 months of age. An inverse relationship was found between theophylline half-life and age. Volume of distribution did not differ from that reported by other authors in similarly aged infants. Our data suggest that childhood clearance rates of elimination can be achieved by 6 months of age. The decreased theophylline elimination observed in the smaller infant indicates that the usual pediatric dosing recommendations cannot be used routinely. Until more specific data are available in the infant under 6 months, the authors reaffirm individualization of theophylline dosage to maintain therapeutic levels and avoid toxicity."}
{"id": "PMID:471676", "title": "Motion parallax as an independent cue for depth perception.", "content": "The perspective transformations of the retinal image, produced by either the movement of an observer or the movement of objects in the visual world, were found to produce a reliable, consistent, and unambiguous impression of relative depth in the absence of all other cues to depth and distance. The stimulus displays consisted of computer-generated random-dot patterns that could be transformed by each movement of the observer or the display oscilloscope to simulate the relative movement information produced by a three-dimensional surface. Using a stereoscopic matching task, the second experiment showed that the perceived depth from parallax transformations is in close agreement with the degree of relative image displacement, as well as producing a compelling impression of three-dimensionality not unlike that found with random-dot stereograms.", "contents": "Motion parallax as an independent cue for depth perception. The perspective transformations of the retinal image, produced by either the movement of an observer or the movement of objects in the visual world, were found to produce a reliable, consistent, and unambiguous impression of relative depth in the absence of all other cues to depth and distance. The stimulus displays consisted of computer-generated random-dot patterns that could be transformed by each movement of the observer or the display oscilloscope to simulate the relative movement information produced by a three-dimensional surface. Using a stereoscopic matching task, the second experiment showed that the perceived depth from parallax transformations is in close agreement with the degree of relative image displacement, as well as producing a compelling impression of three-dimensionality not unlike that found with random-dot stereograms."}
{"id": "PMID:471677", "title": "Depth inversion despite stereopsis: the appearance of random-dot stereograms on surfaces seen in reverse perspective.", "content": "Inside-out relief masks of faces can be depth-inverted (i.e. seen in reverse perspective) during close-up binocular viewing. If a random-dot stereogram is projected onto such a mask, stereopsis can be achieved for the stereogram, and its depth planes are correctly seen while the mask itself, including the region covered by the stereogram, is simultaneously perceived as depth-inverted. This demonstration shows that binocular depth inversion cannot be explained by a complete loss of stereoscopic information (e.g. through monocular suppression), or by a process analogous to pseudoscopic viewing whereby retinal disparities are incorporated into perception, but with their signs uniformly reversed.", "contents": "Depth inversion despite stereopsis: the appearance of random-dot stereograms on surfaces seen in reverse perspective. Inside-out relief masks of faces can be depth-inverted (i.e. seen in reverse perspective) during close-up binocular viewing. If a random-dot stereogram is projected onto such a mask, stereopsis can be achieved for the stereogram, and its depth planes are correctly seen while the mask itself, including the region covered by the stereogram, is simultaneously perceived as depth-inverted. This demonstration shows that binocular depth inversion cannot be explained by a complete loss of stereoscopic information (e.g. through monocular suppression), or by a process analogous to pseudoscopic viewing whereby retinal disparities are incorporated into perception, but with their signs uniformly reversed."}
{"id": "PMID:471678", "title": "The site of binocular rivalry suppression.", "content": "Two experiments were performed to localize the site of binocular rivalry suppression in relation to the locus of grating adaptation. In one experiment it was found that phenomenal suppression of a high-contrast adaptation grating presented to one eye had no influence on the strength of the threshold-elevation aftereffect measured interocularly. Evidently information about the adaptation grating arrives at the site of the aftereffect (presumably binocular neurons) even during suppression. In a second experiment 60 s of grating adaptation was found to produce a short-term reduction in the predominance of the adapted eye during binocular rivalry. These findings provide converging lines of evidence that suppression occurs at a site in the human visual system after the locus of grating adaptation and, hence, after the striate cortex.", "contents": "The site of binocular rivalry suppression. Two experiments were performed to localize the site of binocular rivalry suppression in relation to the locus of grating adaptation. In one experiment it was found that phenomenal suppression of a high-contrast adaptation grating presented to one eye had no influence on the strength of the threshold-elevation aftereffect measured interocularly. Evidently information about the adaptation grating arrives at the site of the aftereffect (presumably binocular neurons) even during suppression. In a second experiment 60 s of grating adaptation was found to produce a short-term reduction in the predominance of the adapted eye during binocular rivalry. These findings provide converging lines of evidence that suppression occurs at a site in the human visual system after the locus of grating adaptation and, hence, after the striate cortex."}
{"id": "PMID:471679", "title": "Does visual texture discrimination precede binocular fusion?", "content": "Various stereoscopic demonstrations are presented which indicate that visual texture discrimination is based on processes which occur after, or at the same time as, the binocular combination of images from the two eyes. Monocularly invisible texture regions can become apparent, and monocularly visible regions can be hidden, by the processes of binocular fusion.", "contents": "Does visual texture discrimination precede binocular fusion? Various stereoscopic demonstrations are presented which indicate that visual texture discrimination is based on processes which occur after, or at the same time as, the binocular combination of images from the two eyes. Monocularly invisible texture regions can become apparent, and monocularly visible regions can be hidden, by the processes of binocular fusion."}
{"id": "PMID:471680", "title": "The contribution of colour to three-dimensional ambiguities in paintings and drawings.", "content": "This paper is about kinds of visual ambiguity that concern the author as a painter. Some effects of incompatible depth cues on the experience of looking at paintings and drawings are discussed. Particular attention is given to the role of colour as a depth cue. It is suggested that areas of identical pigmentation on different parts of a picture surface tend to be interpreted as being on the same plane. Where this interpretation is incompatible with other depth cues 'visual tension' results, the degree of tension being dependent on the relative strength of the cues involved. This hypothesis is illustrated and elaborated with the help of a number of line drawings.", "contents": "The contribution of colour to three-dimensional ambiguities in paintings and drawings. This paper is about kinds of visual ambiguity that concern the author as a painter. Some effects of incompatible depth cues on the experience of looking at paintings and drawings are discussed. Particular attention is given to the role of colour as a depth cue. It is suggested that areas of identical pigmentation on different parts of a picture surface tend to be interpreted as being on the same plane. Where this interpretation is incompatible with other depth cues 'visual tension' results, the degree of tension being dependent on the relative strength of the cues involved. This hypothesis is illustrated and elaborated with the help of a number of line drawings."}
{"id": "PMID:471681", "title": "Adaptation to displacing prisms in human infants.", "content": "This research reports a first attempt to study adaptation to laterally displacing prisms in human infants of ages six to ten months. Twenty infants were tested over four days under conditions similar to those used in adult adaptation studies. Evidence was found of vigorous adaptation when infants were exposed to base-right prisms under conditions of active exposure. Detailed consideration is given to the conditions and methods of studying adaptation in infants and implications for adult studies.", "contents": "Adaptation to displacing prisms in human infants. This research reports a first attempt to study adaptation to laterally displacing prisms in human infants of ages six to ten months. Twenty infants were tested over four days under conditions similar to those used in adult adaptation studies. Evidence was found of vigorous adaptation when infants were exposed to base-right prisms under conditions of active exposure. Detailed consideration is given to the conditions and methods of studying adaptation in infants and implications for adult studies."}
{"id": "PMID:471682", "title": "The relational determination of length illusions and length aftereffects.", "content": "When two lines were presented simultaneously, in an illusion paradigm, the judged length of one of the lines (the focal line) assimilated toward the length of the contextual line. When the lines were presented sequentially, in an aftereffect paradigm, the apparent length of the focal line was displaced away from the length of a previously presented contextual line (i.e. contrast). The largest effects obtained at inverse contextual-to-focal length ratios: 1.67:1 for contextual lines longer than the focal line and 0.60:1 for contextual lines shorter than the focal line. Overestimation of focal length was larger than underestimation for both illusions and aftereffects despite the fact that identical stimuli produced effects in opposite directions in the two paradigms. Results were discussed in terms of a neural length-coding model and were related to visual size constancy.", "contents": "The relational determination of length illusions and length aftereffects. When two lines were presented simultaneously, in an illusion paradigm, the judged length of one of the lines (the focal line) assimilated toward the length of the contextual line. When the lines were presented sequentially, in an aftereffect paradigm, the apparent length of the focal line was displaced away from the length of a previously presented contextual line (i.e. contrast). The largest effects obtained at inverse contextual-to-focal length ratios: 1.67:1 for contextual lines longer than the focal line and 0.60:1 for contextual lines shorter than the focal line. Overestimation of focal length was larger than underestimation for both illusions and aftereffects despite the fact that identical stimuli produced effects in opposite directions in the two paradigms. Results were discussed in terms of a neural length-coding model and were related to visual size constancy."}
{"id": "PMID:471683", "title": "Bias-free improvement in wine discrimination.", "content": "Over three test sessions alternated with two training sessions, accuracy of 'same'/'different' judgments of four dry, white wines improved 14%. Confidence ratings were obtained so that the area under the isosensitivity curve could be computed as a bias-free measure of accuracy while the wine used for same pairs was held constant. An initial bias for 'different' judgments persisted, rather than disappearing as Walk found. While perceptual learning did occur, the problem of attending to irrelevant differences was not overcome.", "contents": "Bias-free improvement in wine discrimination. Over three test sessions alternated with two training sessions, accuracy of 'same'/'different' judgments of four dry, white wines improved 14%. Confidence ratings were obtained so that the area under the isosensitivity curve could be computed as a bias-free measure of accuracy while the wine used for same pairs was held constant. An initial bias for 'different' judgments persisted, rather than disappearing as Walk found. While perceptual learning did occur, the problem of attending to irrelevant differences was not overcome."}
{"id": "PMID:471684", "title": "Sensory conditions for the occurrence of the neon spreading illusion.", "content": "The neon effect, i.e. an illusion of light projected onto a homogeneous lattice, may occur in line patterns made up of elements of different color or brightness. Particular luminance relations between the different line elements in the patterns and between these line elements and their background appear to be critical for the occurrence of the effect.", "contents": "Sensory conditions for the occurrence of the neon spreading illusion. The neon effect, i.e. an illusion of light projected onto a homogeneous lattice, may occur in line patterns made up of elements of different color or brightness. Particular luminance relations between the different line elements in the patterns and between these line elements and their background appear to be critical for the occurrence of the effect."}
{"id": "PMID:471685", "title": "Discrimination of stimulus aperiodicity as a function of temporal patterning: a discrepancy between psychophysical and sensory-neural assessments.", "content": "Tactile discrimination of stimulus aperiodicity was investigated in a two-alternative forced-choice task by having subjects report which of two successively presented trains of mechanical pulses, one with complete pulse periodicity and one in which a single pulse was temporally displaced from its regular placement, was temporally irregular or aperiodic. Trains contained three, four, or five pulses and were presented at interpulse intervals of 60, 75, 85, 100, 125, and 150 ms. Aperiodic trains had one pulse, other than the first or last pulse, temporally advanced or receded by 40% of the interstimulus interval. Results showed that: (i) although the percentage of correct reports of aperiodicity decreased as presentation rate increased (smaller interpulse intervals), the amount of decrease was a function of the number of pulses in a train; (ii) the discrimination of aperiodicity was dependent upon the specific location of the irregularity within the stimulus temporal pattern; and (iii) all patterns with complete temporal reversals of their successive interpulse intervals yielded nearly equivalently accurate reports of aperiodicity. These data suggest that central, attentional factors are involved in the discrimination of stimulus aperiodicity.", "contents": "Discrimination of stimulus aperiodicity as a function of temporal patterning: a discrepancy between psychophysical and sensory-neural assessments. Tactile discrimination of stimulus aperiodicity was investigated in a two-alternative forced-choice task by having subjects report which of two successively presented trains of mechanical pulses, one with complete pulse periodicity and one in which a single pulse was temporally displaced from its regular placement, was temporally irregular or aperiodic. Trains contained three, four, or five pulses and were presented at interpulse intervals of 60, 75, 85, 100, 125, and 150 ms. Aperiodic trains had one pulse, other than the first or last pulse, temporally advanced or receded by 40% of the interstimulus interval. Results showed that: (i) although the percentage of correct reports of aperiodicity decreased as presentation rate increased (smaller interpulse intervals), the amount of decrease was a function of the number of pulses in a train; (ii) the discrimination of aperiodicity was dependent upon the specific location of the irregularity within the stimulus temporal pattern; and (iii) all patterns with complete temporal reversals of their successive interpulse intervals yielded nearly equivalently accurate reports of aperiodicity. These data suggest that central, attentional factors are involved in the discrimination of stimulus aperiodicity."}
{"id": "PMID:471686", "title": "Optical defocusing reverses perceptual organization.", "content": "At a certain point during the defocusing of a reversible figure, the organization of the figure reversed unexpectedly, and the reversal seemed more abrupt than a spontaneous reversal. The critical point is approximately constant over time for a given subject, and a similar point occurs during refocusing. This phenomenon is discussed in terms of inhibition between competing neural structures.", "contents": "Optical defocusing reverses perceptual organization. At a certain point during the defocusing of a reversible figure, the organization of the figure reversed unexpectedly, and the reversal seemed more abrupt than a spontaneous reversal. The critical point is approximately constant over time for a given subject, and a similar point occurs during refocusing. This phenomenon is discussed in terms of inhibition between competing neural structures."}
{"id": "PMID:471690", "title": "Are black circles attentive fields? A reply to Bross, Blair, and Longtin.", "content": "It is argued that the experiment carried out by Bross et al is not a valid test of assimilation theory because they confuse a graphic representation of a constant with the construct itself and assume that changing the former will alter the latter.", "contents": "Are black circles attentive fields? A reply to Bross, Blair, and Longtin. It is argued that the experiment carried out by Bross et al is not a valid test of assimilation theory because they confuse a graphic representation of a constant with the construct itself and assume that changing the former will alter the latter."}
{"id": "PMID:471700", "title": "Knee disarticulation versus above-knee amputation.", "content": "If below-knee amputation is impossible, knee disarticulation should be considered before above-knee amputation, regardless of age and etiology. Knee disarticulation which leaves the femur and patella untoched offers many advantages. The surgical technique is simple and non-traumatic since no bone or muscle tissue is to be dissected. The thigh muscles are completely preserved and thus there is no muscular imbalance. The stump permits total end bearing and its bulbous shape permits easy and firm attachment of the prosthesis. A specially designed double-wall socket and various types of knee joints are presented. Modern prostheses are superior to above-knee prostheses with regard to function, comfort and cosmesis. Results of 72 patients of all age groups are presented and discussed.", "contents": "Knee disarticulation versus above-knee amputation. If below-knee amputation is impossible, knee disarticulation should be considered before above-knee amputation, regardless of age and etiology. Knee disarticulation which leaves the femur and patella untoched offers many advantages. The surgical technique is simple and non-traumatic since no bone or muscle tissue is to be dissected. The thigh muscles are completely preserved and thus there is no muscular imbalance. The stump permits total end bearing and its bulbous shape permits easy and firm attachment of the prosthesis. A specially designed double-wall socket and various types of knee joints are presented. Modern prostheses are superior to above-knee prostheses with regard to function, comfort and cosmesis. Results of 72 patients of all age groups are presented and discussed."}
{"id": "PMID:471701", "title": "Revascularization or amputation.", "content": "The factors which determine the choice, revascularization or amputation of an ischaemic leg are very numerous, variable and sometimes also related. They are concerned with the extent and course of the gangrene, the general condition of the patient and the risk of operation, the technical operability in terms of arterial reconstruction, the skill and judgement of the vascular surgeon, the motivation and life expectancy of the patient, as well as the facilities at the limb-fitting centre. In principle, arterial reconstruction should be the primary consideration in all patients with severe ischaemia of a leg, and threatened with loss of the limb. This implies the need for evaluation by a vascular surgeon. If arterial reconstruction is impossible or undesirable and if lumbar sympathectomy is not indicated either, then if amputation is necessary it must be decided, when is it necessary, and whether a below-knee or an above-knee amputation is possible. The patient with severe arterial circulatory disorders is best served when the vascular surgeon who is responsible for the arterial reconstruction, also assumes responsibility for determining the timing and the level of an amputation, and in some hospitals even for doing the amputation. In other words, the same doctor, preferably the vascular surgeon, has to be responsible for the selection of the patients and the judgement whether the patient is a candidate for revascularization or amputation.", "contents": "Revascularization or amputation. The factors which determine the choice, revascularization or amputation of an ischaemic leg are very numerous, variable and sometimes also related. They are concerned with the extent and course of the gangrene, the general condition of the patient and the risk of operation, the technical operability in terms of arterial reconstruction, the skill and judgement of the vascular surgeon, the motivation and life expectancy of the patient, as well as the facilities at the limb-fitting centre. In principle, arterial reconstruction should be the primary consideration in all patients with severe ischaemia of a leg, and threatened with loss of the limb. This implies the need for evaluation by a vascular surgeon. If arterial reconstruction is impossible or undesirable and if lumbar sympathectomy is not indicated either, then if amputation is necessary it must be decided, when is it necessary, and whether a below-knee or an above-knee amputation is possible. The patient with severe arterial circulatory disorders is best served when the vascular surgeon who is responsible for the arterial reconstruction, also assumes responsibility for determining the timing and the level of an amputation, and in some hospitals even for doing the amputation. In other words, the same doctor, preferably the vascular surgeon, has to be responsible for the selection of the patients and the judgement whether the patient is a candidate for revascularization or amputation."}
{"id": "PMID:471705", "title": "Normal human locomotion.", "content": "A study of normal locomotion requires an understanding of both the movements and the force actions involved. This is equally true in appreciating the problems of pathological gait. The gait cycle is described in terms of the significant events which occur during both the stance and swing phases. The basic principles underlying the analysis of force actions in walking are briefly described. A simple example of force actions in the elbow joint is considered and the analysis extrapolated to provide a general statement regarding locomotion. This relates to the muscle actions required to resist turning actions at joints due to the force effects in walking and the corresponding forces in the joints themselves. The conventional display of information relating to joing actions is considered and compared with the actual situation, \"Stick diagrams\" of motion in the sagittal plane are used to identify and discuss the actions at the joints of the leg in walking. Comparisons are made between this and pathological gait--in particular that of the above-knee amputee.", "contents": "Normal human locomotion. A study of normal locomotion requires an understanding of both the movements and the force actions involved. This is equally true in appreciating the problems of pathological gait. The gait cycle is described in terms of the significant events which occur during both the stance and swing phases. The basic principles underlying the analysis of force actions in walking are briefly described. A simple example of force actions in the elbow joint is considered and the analysis extrapolated to provide a general statement regarding locomotion. This relates to the muscle actions required to resist turning actions at joints due to the force effects in walking and the corresponding forces in the joints themselves. The conventional display of information relating to joing actions is considered and compared with the actual situation, \"Stick diagrams\" of motion in the sagittal plane are used to identify and discuss the actions at the joints of the leg in walking. Comparisons are made between this and pathological gait--in particular that of the above-knee amputee."}
{"id": "PMID:471717", "title": "Studies on seminal vesiculitis in the bull. I. Semen examination methods and post mortem findings.", "content": "During the ten-year period 1968--1978, about 1500 bulls were examined yearly for possible inflammatory conditions in the genital organs. Thirty-one cases of vesiculitis with or without accompanying ampullitis, and one case of isolated, one-sided ampullitis were recorded. Twenty-three cases (72%) were found in young bulls aged 18 months or less. Diagnosis was based on clinical examination of the pelvic genital organs or examination of semen, or in most cases on both. In 17 (53%) of the cases the diagnosis was initially established in the laboratory. Of the laboratory methods, the most reliable was the demonstration of pus cells in Giemsa stained semen smears. Twenty-five cases (24 + one case of ampullitis) were examined post mortem. Fifteen showed interstitial vesiculitis, Group I (Galloway, 1964). The milder 'catarrhal' type (Group II) was found in 9 cases. The 'potential pathogenic' bacteria isolated from the inflamed vesicular glands were: Corynebact. pyogenes, 8 cases (33%), E. coli, 2 cases; Ureaplasma, 2 cases (one in combination with Corynebact. pyogenes). Four of the bulls were newly imported (stress ?), IN THREE BULLS THERE WAS A HISTORY OF NAVEL INFECTION DURING CALFHOOD, AND IN ONE CASE THE BULL HAD LARGE NUMBERS OF VESICAL CALCULI (ABOUT 300 G). In ten of the sets of pelvic organs examined (40%) rare congenital defects or anatomical variations were found in or around the colliculus seminalis (Blom, 1979b).", "contents": "Studies on seminal vesiculitis in the bull. I. Semen examination methods and post mortem findings. During the ten-year period 1968--1978, about 1500 bulls were examined yearly for possible inflammatory conditions in the genital organs. Thirty-one cases of vesiculitis with or without accompanying ampullitis, and one case of isolated, one-sided ampullitis were recorded. Twenty-three cases (72%) were found in young bulls aged 18 months or less. Diagnosis was based on clinical examination of the pelvic genital organs or examination of semen, or in most cases on both. In 17 (53%) of the cases the diagnosis was initially established in the laboratory. Of the laboratory methods, the most reliable was the demonstration of pus cells in Giemsa stained semen smears. Twenty-five cases (24 + one case of ampullitis) were examined post mortem. Fifteen showed interstitial vesiculitis, Group I (Galloway, 1964). The milder 'catarrhal' type (Group II) was found in 9 cases. The 'potential pathogenic' bacteria isolated from the inflamed vesicular glands were: Corynebact. pyogenes, 8 cases (33%), E. coli, 2 cases; Ureaplasma, 2 cases (one in combination with Corynebact. pyogenes). Four of the bulls were newly imported (stress ?), IN THREE BULLS THERE WAS A HISTORY OF NAVEL INFECTION DURING CALFHOOD, AND IN ONE CASE THE BULL HAD LARGE NUMBERS OF VESICAL CALCULI (ABOUT 300 G). In ten of the sets of pelvic organs examined (40%) rare congenital defects or anatomical variations were found in or around the colliculus seminalis (Blom, 1979b)."}
{"id": "PMID:471720", "title": "Functional additive effect of H blood group alleles on carcass length in Danish landrace pigs.", "content": "Previous investigations have revealed statistically significant associations between H blood group genotypes and carcass length in Danish Landrace pigs. The same data were subjected to an analysis aimed at a genetic interpretation of the associations. The regression of the average carcass length (in cm) on the average number of H- alleles for individuals within three genetically characterized classes was 0.725 and the correlation coefficient was 0.995. The results indicate that the associations can be interpreted as being due to an additive effect of the H blood group alleles on carcass length. The additive effect is designated as being functional. A similar effect is expected with respect to any allelic pair exhibiting linkage disequilibrium with alleles at the H locus.", "contents": "Functional additive effect of H blood group alleles on carcass length in Danish landrace pigs. Previous investigations have revealed statistically significant associations between H blood group genotypes and carcass length in Danish Landrace pigs. The same data were subjected to an analysis aimed at a genetic interpretation of the associations. The regression of the average carcass length (in cm) on the average number of H- alleles for individuals within three genetically characterized classes was 0.725 and the correlation coefficient was 0.995. The results indicate that the associations can be interpreted as being due to an additive effect of the H blood group alleles on carcass length. The additive effect is designated as being functional. A similar effect is expected with respect to any allelic pair exhibiting linkage disequilibrium with alleles at the H locus."}
{"id": "PMID:471721", "title": "[Diagnosis of cerebral hemorrhages. A report of 247 anatomo-pathological cases (author's transl)].", "content": "A series of 247 cases with simple and apparently primitive cerebral hemorrhages selected from the pathological records of Charles Foix laboratory from 1962 to 1977 is reviewed with regard to the accuracy of the premortem diagnosis as a function of the size, the location and the age of the bleeding, the patient's age, the practices of angiography or lumbar puncture and the considered period of death. The clinical diagnosis of cerebral vascular disease was made in 75% of the cases from that serie but the hemorrhagic mechanism was identified only in 50,6% of cases. The diagnosis accuracy was enhanced in large and recent hemorrhages occuring in patients under 70 years old. It seemed better in cerebellar and intermediate locations. It was enhanced in more recent observations (collected since 1974). The practice of cerebral angiography and lumbar puncture rose up (in a somewhat equivalent way) the proportion of accurate diagnoses. The lumbar puncture practice was not associated with a higher rate of cerebral herniae.", "contents": "[Diagnosis of cerebral hemorrhages. A report of 247 anatomo-pathological cases (author's transl)]. A series of 247 cases with simple and apparently primitive cerebral hemorrhages selected from the pathological records of Charles Foix laboratory from 1962 to 1977 is reviewed with regard to the accuracy of the premortem diagnosis as a function of the size, the location and the age of the bleeding, the patient's age, the practices of angiography or lumbar puncture and the considered period of death. The clinical diagnosis of cerebral vascular disease was made in 75% of the cases from that serie but the hemorrhagic mechanism was identified only in 50,6% of cases. The diagnosis accuracy was enhanced in large and recent hemorrhages occuring in patients under 70 years old. It seemed better in cerebellar and intermediate locations. It was enhanced in more recent observations (collected since 1974). The practice of cerebral angiography and lumbar puncture rose up (in a somewhat equivalent way) the proportion of accurate diagnoses. The lumbar puncture practice was not associated with a higher rate of cerebral herniae."}
{"id": "PMID:471722", "title": "[Vascular risk factors in chronic haemodialysis: the role of HDL cholesterol. Correlation with dietary intake and the quality of dialysis (author's transl)].", "content": "HDL cholesterol in 53 hemodialysed patients is significantly reduced (p less than 0,001) in front of 34 healthy people. The decrease is more pronounced in male but does not seem influenced by age or ethnical origin of subjects. There is no correlation between alpha cholesterol levels and hyperlipidemia which are in 50% of cases of type IV and more frequent in caucasian than in african: the beta cholesterol is correlated with total cholesterol and increased in type IV (p less than 0,001) but alpha cholesterol negatively correlated with triglyceridemia (p less than 0,001) is in comparable levels in dialysed with type IV or without hyperlipidemia. If for normal value triglyceridemia is correlated with caloric and carbohydrate intake (p less than 0,001), the diet does not seem to influence either the frequence of type IV nor the level of alpha cholesterol. However we found a positive correlation (p less than 0,05) between nervous conduction velocity and HDL cholesterol, this fact can let us hope a decrease in the cardiovascular complication associated with amelioration of the adequacy of dialysis.", "contents": "[Vascular risk factors in chronic haemodialysis: the role of HDL cholesterol. Correlation with dietary intake and the quality of dialysis (author's transl)]. HDL cholesterol in 53 hemodialysed patients is significantly reduced (p less than 0,001) in front of 34 healthy people. The decrease is more pronounced in male but does not seem influenced by age or ethnical origin of subjects. There is no correlation between alpha cholesterol levels and hyperlipidemia which are in 50% of cases of type IV and more frequent in caucasian than in african: the beta cholesterol is correlated with total cholesterol and increased in type IV (p less than 0,001) but alpha cholesterol negatively correlated with triglyceridemia (p less than 0,001) is in comparable levels in dialysed with type IV or without hyperlipidemia. If for normal value triglyceridemia is correlated with caloric and carbohydrate intake (p less than 0,001), the diet does not seem to influence either the frequence of type IV nor the level of alpha cholesterol. However we found a positive correlation (p less than 0,05) between nervous conduction velocity and HDL cholesterol, this fact can let us hope a decrease in the cardiovascular complication associated with amelioration of the adequacy of dialysis."}
{"id": "PMID:471723", "title": "[Systematic neonatal screening for Duchenne muscular dystrophy].", "content": "More than 15% of cases of Duchenne muscular dystrophy (DMD) may be preventable by the neonatal diagnosis of another affected relative. Systematic neonatal screening seems a more efficient way of getting information for genetic counseling. The principle of the test described is the detection of a specific increase of the activity of Creatine-Kinase (CK) in the blood of newborn children with myopathy. Blood may be spotted on paper and posted to a central laboratory. Furthermore this program can be integrated into the systematic screening of phenylketonurie. The specificity and accuracy of the bioluminescent reaction used for the evaluation of CK suggest that this screening reaction is reliable. A systematic neonatal screening program for DMD such as ours in the Rh\u00f4ne-Alpes area (France) will lead to frequent recourse to prenatal diagnosis now possible through sex fetal determination and which would be possible through in utero blood sampling.", "contents": "[Systematic neonatal screening for Duchenne muscular dystrophy]. More than 15% of cases of Duchenne muscular dystrophy (DMD) may be preventable by the neonatal diagnosis of another affected relative. Systematic neonatal screening seems a more efficient way of getting information for genetic counseling. The principle of the test described is the detection of a specific increase of the activity of Creatine-Kinase (CK) in the blood of newborn children with myopathy. Blood may be spotted on paper and posted to a central laboratory. Furthermore this program can be integrated into the systematic screening of phenylketonurie. The specificity and accuracy of the bioluminescent reaction used for the evaluation of CK suggest that this screening reaction is reliable. A systematic neonatal screening program for DMD such as ours in the Rh\u00f4ne-Alpes area (France) will lead to frequent recourse to prenatal diagnosis now possible through sex fetal determination and which would be possible through in utero blood sampling."}
{"id": "PMID:471724", "title": "[Hydroxy 9-methyl 2-ellipticinium acetate (NSC 264-137). Toxicologic study and therapeutic effect in 100 cancers (author's transl)].", "content": "A new derivative of ellipticine, hydroxy-9-methyl-2-ellipticinium acetate, was found to be a useful anti-tumor drug in advanced cancers which could not be treated any longer successfully by any other procedure. In our series of 100 patients, the best results were obtained with bone metastases from breast carcinomas and with anaplastic thyroid carcinomas. Most patients usually received a weekly perfusion of 80 mg/m2. The main characteristic of this drug is its lack of hematologic, and hepatic toxicity. No renal trouble was observed during the first year, but 2 deaths from renal insufficiency occured during the 18th and 15th month of treatment. The most frequent side effect consists of digestive troubles (nausea, vomiting) which rarely compelled to stop the treatment (4 times in 100 patients).", "contents": "[Hydroxy 9-methyl 2-ellipticinium acetate (NSC 264-137). Toxicologic study and therapeutic effect in 100 cancers (author's transl)]. A new derivative of ellipticine, hydroxy-9-methyl-2-ellipticinium acetate, was found to be a useful anti-tumor drug in advanced cancers which could not be treated any longer successfully by any other procedure. In our series of 100 patients, the best results were obtained with bone metastases from breast carcinomas and with anaplastic thyroid carcinomas. Most patients usually received a weekly perfusion of 80 mg/m2. The main characteristic of this drug is its lack of hematologic, and hepatic toxicity. No renal trouble was observed during the first year, but 2 deaths from renal insufficiency occured during the 18th and 15th month of treatment. The most frequent side effect consists of digestive troubles (nausea, vomiting) which rarely compelled to stop the treatment (4 times in 100 patients)."}
{"id": "PMID:471733", "title": "[Iodine overloads in amniotic fluids (author's transl)].", "content": "The mean of total iodine levels in amniotic fluid samples is 7.8 ng/ml during the middle of pregnancy and 4.4 ng at the end. On about 200 samples collected at random from high risk pregnant women, 14% fluid contain iodine load. This iodine excess comes from water-soluble iodinated drugs administrated to the pregnant women; most are less than 300 ng/ml but 24 hours after urography the load reaches 80,000 times the mean value. Biochemical analyses have shown the iodine excess was iodide, easely released. In our study no neonatal hypothyroidism was noticed probably because that degradation. However administration of any iodinated drug to pregnant women may be a risk to the fetus.", "contents": "[Iodine overloads in amniotic fluids (author's transl)]. The mean of total iodine levels in amniotic fluid samples is 7.8 ng/ml during the middle of pregnancy and 4.4 ng at the end. On about 200 samples collected at random from high risk pregnant women, 14% fluid contain iodine load. This iodine excess comes from water-soluble iodinated drugs administrated to the pregnant women; most are less than 300 ng/ml but 24 hours after urography the load reaches 80,000 times the mean value. Biochemical analyses have shown the iodine excess was iodide, easely released. In our study no neonatal hypothyroidism was noticed probably because that degradation. However administration of any iodinated drug to pregnant women may be a risk to the fetus."}
{"id": "PMID:471734", "title": "[Deviation in cortisol metabolism induced by rifampicin. Therapeutic consequences in adrenal failure (author's transl)].", "content": "Deviation of cortisol metabolism in favour of its 6 beta-hydroxylated derivative was demonstrated in two patients with adrenal failure receiving substitution corticosteroid therapy and rifampicin. The existence of a frank increase in the metabolic clearance antipyrin was in favour of an hepatic enzyme induction. After rifampicin treatment was stopped, the 24 hour urinary excretion of 6 beta-OH-F returned to normal, demonstrating the responsability of the drug. This enzyme induction results in a need to increase the dose of hydrocortisone substitution therapy in patients with Addison's disease treated with rifampicin.", "contents": "[Deviation in cortisol metabolism induced by rifampicin. Therapeutic consequences in adrenal failure (author's transl)]. Deviation of cortisol metabolism in favour of its 6 beta-hydroxylated derivative was demonstrated in two patients with adrenal failure receiving substitution corticosteroid therapy and rifampicin. The existence of a frank increase in the metabolic clearance antipyrin was in favour of an hepatic enzyme induction. After rifampicin treatment was stopped, the 24 hour urinary excretion of 6 beta-OH-F returned to normal, demonstrating the responsability of the drug. This enzyme induction results in a need to increase the dose of hydrocortisone substitution therapy in patients with Addison's disease treated with rifampicin."}
{"id": "PMID:471735", "title": "[Computerized tomography: physiological reheightering of the images by sugars (author's transl)].", "content": "During experimental utilization on the dog (per os) of several types of materials capable to be modified by the liver after digestive absorption, sugars appeared naturally opaques in the gastric cavity and so they could substitute in C.T. to the habitual materials of contrast, we also notice some belated modifications on intra hepatic density that might be in relation with the metabolism of intra hepatic glucose.", "contents": "[Computerized tomography: physiological reheightering of the images by sugars (author's transl)]. During experimental utilization on the dog (per os) of several types of materials capable to be modified by the liver after digestive absorption, sugars appeared naturally opaques in the gastric cavity and so they could substitute in C.T. to the habitual materials of contrast, we also notice some belated modifications on intra hepatic density that might be in relation with the metabolism of intra hepatic glucose."}
{"id": "PMID:471736", "title": "[Hydatic cyst of the thyroid. One case (author's transl)].", "content": "Thyroid localisation of an hydatic cyst is a rather rare phenomenon. Therefore, the occurence in our region of a thyroid hydatic cyst is noteworthy. In the present observation, the needle aspiration of an hypofixating nodule assured the diagnosis. Further more, this technique was harmless.", "contents": "[Hydatic cyst of the thyroid. One case (author's transl)]. Thyroid localisation of an hydatic cyst is a rather rare phenomenon. Therefore, the occurence in our region of a thyroid hydatic cyst is noteworthy. In the present observation, the needle aspiration of an hypofixating nodule assured the diagnosis. Further more, this technique was harmless."}
{"id": "PMID:471737", "title": "[Bacterial endocarditis due to Staphylococcus epidermidis. Two cases (author's transl)].", "content": "Bacterial endocarditis due to Staphylococcus epermidis is rare and severe. In a first patient, a 58-year-old-man, it developed 40 days after the insertion of a double prosthesis (mitral and aortic). Cure was obtained by medical treatment using a combination of vancomycin and gentamicin, followed by pristinamycin and tobramycin. The second patient, a 50-year-old-woman, suffering from cirrhogenic hepatitis and treated with corticosteroids. Staphylococcus epidermidis endocarditis developed without any portal of entry being discovered. After the failure of various antibiotic combinations (even though bactericidal in vitro), a mitral Starr valve was inserted which resulted in cure. None of the patients showed any sign of valvular mutilations or disinsertion of prosthesis.", "contents": "[Bacterial endocarditis due to Staphylococcus epidermidis. Two cases (author's transl)]. Bacterial endocarditis due to Staphylococcus epermidis is rare and severe. In a first patient, a 58-year-old-man, it developed 40 days after the insertion of a double prosthesis (mitral and aortic). Cure was obtained by medical treatment using a combination of vancomycin and gentamicin, followed by pristinamycin and tobramycin. The second patient, a 50-year-old-woman, suffering from cirrhogenic hepatitis and treated with corticosteroids. Staphylococcus epidermidis endocarditis developed without any portal of entry being discovered. After the failure of various antibiotic combinations (even though bactericidal in vitro), a mitral Starr valve was inserted which resulted in cure. None of the patients showed any sign of valvular mutilations or disinsertion of prosthesis."}
{"id": "PMID:471738", "title": "[Corticosteroid osteoporosis (author's transl)].", "content": "Otseoporosis is a common and sometimes severe complication of all prolonged corticosteroid therapy. After reviewing the clinical, radiological, histological and biological features, the authors discuss the various physiopathogenic mechanisms: anti-anabolic effect of corticosteroids and decreased bone formation, and hyperparathyroidism secondary to the calcium deficiency induced by corticosteroids (fall in intestinal calcium absorption). Treatment should be preventive, based upon great car in the prescription of corticosteroids (absolute indication, minimal effective dose, alternate administration in certain cases) and upon improved calcium balance (calcium, vitamin D or its derivatives).", "contents": "[Corticosteroid osteoporosis (author's transl)]. Otseoporosis is a common and sometimes severe complication of all prolonged corticosteroid therapy. After reviewing the clinical, radiological, histological and biological features, the authors discuss the various physiopathogenic mechanisms: anti-anabolic effect of corticosteroids and decreased bone formation, and hyperparathyroidism secondary to the calcium deficiency induced by corticosteroids (fall in intestinal calcium absorption). Treatment should be preventive, based upon great car in the prescription of corticosteroids (absolute indication, minimal effective dose, alternate administration in certain cases) and upon improved calcium balance (calcium, vitamin D or its derivatives)."}
{"id": "PMID:471742", "title": "[Study of the effect of dopamine on renal excretion in chronic renal failure (author's transl)].", "content": "The effects of dopamine on renal excretory function were studied in 20 patients with chronic renal failure (plasma creatinine higher than 30 mg/i). Effects were marked at a dose of 2 micrograms/kg/mn and were no greater at a dose of 5 micrograms/kg/mn (10 subjects in each case). In general, creatinine clearance increased by 28%, urea excretion by 45%, urine output by 77% and urinary sodium output by 164%. In ten patiens with terminal renal failure (plasma creatinine greater than 100 mg/i), the effects of dopamine were maintained. In these individuals, furosemide (250 mg by single intravenous injection) resulted in a higher total urine and urinary sodium output but had a less favourable effect upon creatinine.", "contents": "[Study of the effect of dopamine on renal excretion in chronic renal failure (author's transl)]. The effects of dopamine on renal excretory function were studied in 20 patients with chronic renal failure (plasma creatinine higher than 30 mg/i). Effects were marked at a dose of 2 micrograms/kg/mn and were no greater at a dose of 5 micrograms/kg/mn (10 subjects in each case). In general, creatinine clearance increased by 28%, urea excretion by 45%, urine output by 77% and urinary sodium output by 164%. In ten patiens with terminal renal failure (plasma creatinine greater than 100 mg/i), the effects of dopamine were maintained. In these individuals, furosemide (250 mg by single intravenous injection) resulted in a higher total urine and urinary sodium output but had a less favourable effect upon creatinine."}
{"id": "PMID:471743", "title": "[Automaton resuscitation (author's transl)].", "content": "The aim of the Automaton Resuscitation is execution, watching and maintenance of a programme of intricate resuscitation tying for the first time the therapeutic to extemporaneous outflow of biological spoliation. This apparatus executes permanently and automatically the taking of biological fluid, estimates its outflow, amounts its total and realizes or the reinstillation of the fluid in the digestive tract or the order of intravenous perfusion tied to fluid spoliation according to an adjustable connection. A first self acting regulator for the juice intestinal reinstillation has been made in 1974. The second one with 4 units of continuous aspiration, data integration, reinstillation and perfusion tied with security had waked for 6 months. Moreover it allows with fiability the reinstillation of the gastric, duodenal, bilious, pancreatic or intestinal juice, on the other hand an intravenous perfusion tied to spontaneous spoliation (digestive) or instigated spoliation (provocated diuresis) and in a fundamental way simplifies the work of the physicians and the nurses.", "contents": "[Automaton resuscitation (author's transl)]. The aim of the Automaton Resuscitation is execution, watching and maintenance of a programme of intricate resuscitation tying for the first time the therapeutic to extemporaneous outflow of biological spoliation. This apparatus executes permanently and automatically the taking of biological fluid, estimates its outflow, amounts its total and realizes or the reinstillation of the fluid in the digestive tract or the order of intravenous perfusion tied to fluid spoliation according to an adjustable connection. A first self acting regulator for the juice intestinal reinstillation has been made in 1974. The second one with 4 units of continuous aspiration, data integration, reinstillation and perfusion tied with security had waked for 6 months. Moreover it allows with fiability the reinstillation of the gastric, duodenal, bilious, pancreatic or intestinal juice, on the other hand an intravenous perfusion tied to spontaneous spoliation (digestive) or instigated spoliation (provocated diuresis) and in a fundamental way simplifies the work of the physicians and the nurses."}
{"id": "PMID:471744", "title": "[Acute fatty infiltration of the liver in pregnancy. One case (author's transl)].", "content": "Acute fatty infiltration of the liver in pregnancy is characterised by microvacuolar fatty infiltration, without necrosis, occuring at the end of pregnancy. This syndrome, defined by Sheehan in 1940, remains rare. The authors were able to find 62 authentic cases in the french, english and german literature. The course is fatal in 75% of cases for the mother and 70% for the child. The gravity is related to hepatocellular failure, but also to extra-hepatic complications (renal failure, haemorrhagic syndrome, infectious complications, acute haemorrhagic and/or necrosing pancreatitis). When the course is favourable, the hepatic lesions disappear in a few weeks and there is no recurrence during subsequent pregnancies. The histological lesions of acute fatty infiltration of the liver in pregnancy are identical to those of fatty infiltration of the liver induced by cyclines and of Reye syndrome.", "contents": "[Acute fatty infiltration of the liver in pregnancy. One case (author's transl)]. Acute fatty infiltration of the liver in pregnancy is characterised by microvacuolar fatty infiltration, without necrosis, occuring at the end of pregnancy. This syndrome, defined by Sheehan in 1940, remains rare. The authors were able to find 62 authentic cases in the french, english and german literature. The course is fatal in 75% of cases for the mother and 70% for the child. The gravity is related to hepatocellular failure, but also to extra-hepatic complications (renal failure, haemorrhagic syndrome, infectious complications, acute haemorrhagic and/or necrosing pancreatitis). When the course is favourable, the hepatic lesions disappear in a few weeks and there is no recurrence during subsequent pregnancies. The histological lesions of acute fatty infiltration of the liver in pregnancy are identical to those of fatty infiltration of the liver induced by cyclines and of Reye syndrome."}
{"id": "PMID:471745", "title": "[Complications of digestive involvement in CRST syndrome. 3 cases (author's transl)].", "content": "In CRST syndrome, latent oesophageal involvement is common but not usually associated with any clinical manifestations. Involvement of the small intestine is rare. In three patients there occurred severe complications related to digestive involvement in the condition. In one case, involvement of the small intestine was associated with functional obstruction. In the other two patients, oesophageal involvement progressed to very tight stenosis of the lower end of the eosophagus. The generally accepted bening nature of this form of scleroderma must therefore be questioned.", "contents": "[Complications of digestive involvement in CRST syndrome. 3 cases (author's transl)]. In CRST syndrome, latent oesophageal involvement is common but not usually associated with any clinical manifestations. Involvement of the small intestine is rare. In three patients there occurred severe complications related to digestive involvement in the condition. In one case, involvement of the small intestine was associated with functional obstruction. In the other two patients, oesophageal involvement progressed to very tight stenosis of the lower end of the eosophagus. The generally accepted bening nature of this form of scleroderma must therefore be questioned."}
{"id": "PMID:471746", "title": "[Cervical sprains and cervical herniated discs (author's transl)].", "content": "The cervical spine, containing twenty-articulations, is particularly exposed to sprains. Benign cases are those in which both spinal stability is not affected and only subjective neurovascular complaints are present. Management essentially involves constant volontary immobilization of the cervical spine with or without apparatus (Postural training). The severity of the sprains results from the possibility of either vertebral instability or objective neurovascular complications. Bony instability can be detected on dynamic radiographs based on precise criteria defined by physiologic study of normal spinal movements. When a persistant vascular syndrome involving the vertebro-basilar arteries is observed, vertebral arteriography may be indicated. Likewise, the presence of a thoracic outlet syndrome may required sub-clavian arteriography. Metrizamide myelography is used to explore neurologic lesions. The author concluded that only severe cervical sprains required surgical intervention. A prolapsed cervical disc, revealed by discography and especially metrizamide myelography, most often requires an anterior approach to surgery.", "contents": "[Cervical sprains and cervical herniated discs (author's transl)]. The cervical spine, containing twenty-articulations, is particularly exposed to sprains. Benign cases are those in which both spinal stability is not affected and only subjective neurovascular complaints are present. Management essentially involves constant volontary immobilization of the cervical spine with or without apparatus (Postural training). The severity of the sprains results from the possibility of either vertebral instability or objective neurovascular complications. Bony instability can be detected on dynamic radiographs based on precise criteria defined by physiologic study of normal spinal movements. When a persistant vascular syndrome involving the vertebro-basilar arteries is observed, vertebral arteriography may be indicated. Likewise, the presence of a thoracic outlet syndrome may required sub-clavian arteriography. Metrizamide myelography is used to explore neurologic lesions. The author concluded that only severe cervical sprains required surgical intervention. A prolapsed cervical disc, revealed by discography and especially metrizamide myelography, most often requires an anterior approach to surgery."}
{"id": "PMID:471755", "title": "[Factors influencing the elimination of the migration of inhaled mineral particles (author's transl)].", "content": "The reaction of the body to inhaled mineral dust brings into play physiological and pathological mechanisms in which immunological processes may be involved. An analysis is made of phagocytosis by alveolar macrophages, of the transport of dust to the lymphatic system, of dust aggravation in lymphoid tissues on lymph glands and of the resulting lesions. The part played by dust in immunological disturbances is put forward in the cases of tubercular infections, experimental arthritis and tumors.", "contents": "[Factors influencing the elimination of the migration of inhaled mineral particles (author's transl)]. The reaction of the body to inhaled mineral dust brings into play physiological and pathological mechanisms in which immunological processes may be involved. An analysis is made of phagocytosis by alveolar macrophages, of the transport of dust to the lymphatic system, of dust aggravation in lymphoid tissues on lymph glands and of the resulting lesions. The part played by dust in immunological disturbances is put forward in the cases of tubercular infections, experimental arthritis and tumors."}
{"id": "PMID:471756", "title": "[Gaseous aerocontaminants and phagocytic defence of the respiratory tract. Cytotoxicity of NO2, of ozone and acrolein for alveolar macrophages in gaseous phase (author's transl)].", "content": "A new method of survival in vitro of alveolar macrophages in gaseous phase, ensuring direct contact between the cells and the surrounding atmosphere may be used for the qualitative and quantitative study of the cytotoxicity of gases under controlled experimental conditions. Exposure to nitrous oxide (0.1 to 6 ppm), ozone (0.65 to 3.25 ppm) and to acrolein (4 to 35 ppm) for 30 minutes results in a significant untoward effect on the cells which is proportional to the concentration of the toxic agent. This method offers the possibility of a new approach to study of the mode of action of gaseous aerocontaminants on the respiratory tract and particularly upon phagocytic defences.", "contents": "[Gaseous aerocontaminants and phagocytic defence of the respiratory tract. Cytotoxicity of NO2, of ozone and acrolein for alveolar macrophages in gaseous phase (author's transl)]. A new method of survival in vitro of alveolar macrophages in gaseous phase, ensuring direct contact between the cells and the surrounding atmosphere may be used for the qualitative and quantitative study of the cytotoxicity of gases under controlled experimental conditions. Exposure to nitrous oxide (0.1 to 6 ppm), ozone (0.65 to 3.25 ppm) and to acrolein (4 to 35 ppm) for 30 minutes results in a significant untoward effect on the cells which is proportional to the concentration of the toxic agent. This method offers the possibility of a new approach to study of the mode of action of gaseous aerocontaminants on the respiratory tract and particularly upon phagocytic defences."}
{"id": "PMID:471765", "title": "[Thyroid nodule secondary to subacute thyroiditis. 8 cases (author's transl)].", "content": "This description of 8 cases of thyroid nodules secondary to subacute thyroiditis serves to emphasise the relative prevalence of the circumscribed forms. These nodules, variable in size, firm and painful, developed after a period of cervical discomfort and pain. A contrast between a raised or normal blood hormone level and a very low uptake of I131 was found in five cases. It was all the more valuable when there was no iodine overload. Scintigraphy showed the nodule to be cold in 6 cases, isofixing in one and hot in one case. An essential diagnostic criterion is the rapidly favourable course: 4 nodules disappeared completely, 3 regressed in less than 6 months and one persisted, justifying excision with histopathological examination since localised thyroiditis of this type may be the presenting feature of a carcinoma.", "contents": "[Thyroid nodule secondary to subacute thyroiditis. 8 cases (author's transl)]. This description of 8 cases of thyroid nodules secondary to subacute thyroiditis serves to emphasise the relative prevalence of the circumscribed forms. These nodules, variable in size, firm and painful, developed after a period of cervical discomfort and pain. A contrast between a raised or normal blood hormone level and a very low uptake of I131 was found in five cases. It was all the more valuable when there was no iodine overload. Scintigraphy showed the nodule to be cold in 6 cases, isofixing in one and hot in one case. An essential diagnostic criterion is the rapidly favourable course: 4 nodules disappeared completely, 3 regressed in less than 6 months and one persisted, justifying excision with histopathological examination since localised thyroiditis of this type may be the presenting feature of a carcinoma."}
{"id": "PMID:471766", "title": "[Raynaud's phenomenone: prospective study of 100 cases (author's transl)].", "content": "A prospective study of 100 consecutive patients hospitalised with a Raynaud phenomenone is presented. In 82 patients, a precise etiology was pound, most often a collagen disease (50 cases) and amongst this group generalised scleroderma was the most commun (n = 37). Other etiologies were very divers: inflamatory artertis, atheroma, local mecanical causes, emboligenic. In 18 patients, no etiology was found: the age, the sex, the context and the benign cours in this patients was such that a diagnosis of idiopathic Raynaud's phenomenone. This results lead to the suggestion of a method for the investigation of Raynaud's phenomenone. The consequences are therapeutic, the etiological context foring the base of the treatment prescribe.", "contents": "[Raynaud's phenomenone: prospective study of 100 cases (author's transl)]. A prospective study of 100 consecutive patients hospitalised with a Raynaud phenomenone is presented. In 82 patients, a precise etiology was pound, most often a collagen disease (50 cases) and amongst this group generalised scleroderma was the most commun (n = 37). Other etiologies were very divers: inflamatory artertis, atheroma, local mecanical causes, emboligenic. In 18 patients, no etiology was found: the age, the sex, the context and the benign cours in this patients was such that a diagnosis of idiopathic Raynaud's phenomenone. This results lead to the suggestion of a method for the investigation of Raynaud's phenomenone. The consequences are therapeutic, the etiological context foring the base of the treatment prescribe."}
{"id": "PMID:471767", "title": "[Revascularisation of the intestinal arteries from the suprarenal aorta (author's transl)].", "content": "Amongst procedures for revascularisation of the intestinal arteries, bypass implanted in the suprarenal aorta by a purely abdominal approach, with section of the right crus of the diaphragm, would appear to be the most satisfactory procedure. For the superior mesenteric, in particular, the pre-pancreatic anterograde course avoids any bending or kinking. Nine patinets undergoing surgery with venous or goretex grafts confirmed the quality of the results obtained by this technique.", "contents": "[Revascularisation of the intestinal arteries from the suprarenal aorta (author's transl)]. Amongst procedures for revascularisation of the intestinal arteries, bypass implanted in the suprarenal aorta by a purely abdominal approach, with section of the right crus of the diaphragm, would appear to be the most satisfactory procedure. For the superior mesenteric, in particular, the pre-pancreatic anterograde course avoids any bending or kinking. Nine patinets undergoing surgery with venous or goretex grafts confirmed the quality of the results obtained by this technique."}
{"id": "PMID:471775", "title": "[Results of emission computerised axial tomography of the liver in 113 patients (author's transl)].", "content": "Transversal emission computerised axial tomography (ECAT) was performed in addition to bioptic examination in 113 patients with solid tumours and lyphomas. In 69/113 (61%) patients the results of conventional scintigraphy and ECAT were in agreement. In 44/113 (39%) patients additional information was obtained. Correct positive and correct negative results were found in 86.3% (63.6% and 22.7%, respectively), while in 13.6% the results were false positive or false negative (6.8% both).", "contents": "[Results of emission computerised axial tomography of the liver in 113 patients (author's transl)]. Transversal emission computerised axial tomography (ECAT) was performed in addition to bioptic examination in 113 patients with solid tumours and lyphomas. In 69/113 (61%) patients the results of conventional scintigraphy and ECAT were in agreement. In 44/113 (39%) patients additional information was obtained. Correct positive and correct negative results were found in 86.3% (63.6% and 22.7%, respectively), while in 13.6% the results were false positive or false negative (6.8% both)."}
{"id": "PMID:471776", "title": "[Results of hepatic scintigraphy with a new colloid (99mTc-Sn-Antipyrine D 77 1268) compared with 99mTc-Sn-phytate (author's transl)].", "content": "Liver scans were performed on 84 patients, using a new colloidal substance 99mTc-Sn-Antipyrine colloid, and compared with 36 liver scans with 99mTc-Sn-phytate under identical conditions. The following differences were found: 99mTc-Antipyrine uptake in the spleen was higher in comparison with 99mTc-phytate, renal excretion was twenty times lower. The tin content was less then one fifth in the Sn-Antipyrine preparation. Antipyrine yields simultaneous imaging of liver and spleen even in normal patients.", "contents": "[Results of hepatic scintigraphy with a new colloid (99mTc-Sn-Antipyrine D 77 1268) compared with 99mTc-Sn-phytate (author's transl)]. Liver scans were performed on 84 patients, using a new colloidal substance 99mTc-Sn-Antipyrine colloid, and compared with 36 liver scans with 99mTc-Sn-phytate under identical conditions. The following differences were found: 99mTc-Antipyrine uptake in the spleen was higher in comparison with 99mTc-phytate, renal excretion was twenty times lower. The tin content was less then one fifth in the Sn-Antipyrine preparation. Antipyrine yields simultaneous imaging of liver and spleen even in normal patients."}
{"id": "PMID:471777", "title": "[Effects of venous compression for blood sampling on the results of in vitro tests of thyroid function (author's transl)].", "content": "In a group of subjects with a normal as well as pathological thyroid function there was a significant increase in RT3U, serum-thyroxine-concentrations and TSH-levels but there were no significant changes in the T4-RT3U-indices and ETR-tests after 5 min of venous compression. The discordant results were obtained in 21 cases (26.9%) with the RT3U-test and in 4 cases (5,1%) with the T4-serum-level-determination, which may cause diagnostic errors. The non-standardized venous compression time causes a widening of the euthyroid zone which reduces the discrimination capabilities of these function tests. With TSH-determinations these deviations may be of diagnostic importance in the evaluation of TRH-TSH-test results. A standardized and minimum compression time when drawing blood samples is recommended in order to eliminate this potential source of diagnostic error.", "contents": "[Effects of venous compression for blood sampling on the results of in vitro tests of thyroid function (author's transl)]. In a group of subjects with a normal as well as pathological thyroid function there was a significant increase in RT3U, serum-thyroxine-concentrations and TSH-levels but there were no significant changes in the T4-RT3U-indices and ETR-tests after 5 min of venous compression. The discordant results were obtained in 21 cases (26.9%) with the RT3U-test and in 4 cases (5,1%) with the T4-serum-level-determination, which may cause diagnostic errors. The non-standardized venous compression time causes a widening of the euthyroid zone which reduces the discrimination capabilities of these function tests. With TSH-determinations these deviations may be of diagnostic importance in the evaluation of TRH-TSH-test results. A standardized and minimum compression time when drawing blood samples is recommended in order to eliminate this potential source of diagnostic error."}
{"id": "PMID:471778", "title": "Radioiodide treatment of pulmonary metastases of differentiated thyroid cancer. Results and prognostic factors.", "content": "Pulmonary metastases were found in 123 out of 840 patients with thyroid cancer between 1955-1977, 87 patients with pulmonary metastases of differentiated cancer were studied in detail, including an evaluation of prognostically important factors. In 66 of them, the induction of 131I uptake in metastases was attempted, in half of them successfully. Uptake was achieved more frequently in younger subjects, in papillary cancers and in patients with fine pulmonary metastases on chest films. Survival (not corrected for age) was evaluated 10 and 15 years following the diagnosis of thyroid cancer and was found to be 29,1% and 12,2%, respectively. Significantly higher survival rates were seen in younger patients, in patients with the fine type of pulmonary metastases, in the absence of bone metastases and, particularly, in patients with induced 131I uptake in metastases. Papillary cancers were found to have higher survival rates in males and in young subjects only, in the whole group the survival rates were independent of either microscopic type or sex. It is believed that biologic behaviour of distant (pulmonary) metastases may be influenced by radiodide therapy.", "contents": "Radioiodide treatment of pulmonary metastases of differentiated thyroid cancer. Results and prognostic factors. Pulmonary metastases were found in 123 out of 840 patients with thyroid cancer between 1955-1977, 87 patients with pulmonary metastases of differentiated cancer were studied in detail, including an evaluation of prognostically important factors. In 66 of them, the induction of 131I uptake in metastases was attempted, in half of them successfully. Uptake was achieved more frequently in younger subjects, in papillary cancers and in patients with fine pulmonary metastases on chest films. Survival (not corrected for age) was evaluated 10 and 15 years following the diagnosis of thyroid cancer and was found to be 29,1% and 12,2%, respectively. Significantly higher survival rates were seen in younger patients, in patients with the fine type of pulmonary metastases, in the absence of bone metastases and, particularly, in patients with induced 131I uptake in metastases. Papillary cancers were found to have higher survival rates in males and in young subjects only, in the whole group the survival rates were independent of either microscopic type or sex. It is believed that biologic behaviour of distant (pulmonary) metastases may be influenced by radiodide therapy."}
{"id": "PMID:471779", "title": "[The diagnosis of cerebrospinal rhinorrhea with the 131I-hippuran swab test].", "content": "The report deals with the 131I-hippuran swab test and its effectiveness as compared with other methods (X-ray techniques, Clinistix) in 123 patients with rhinoliquorrhea. Results in 32 patients with rhinoliquorrhea are correlated with those of various methods of examination and with the surgical reports. In frontal and ethmoidal lesions and in those of the middle part of the face the liquorrheas can be demonstrated with a greater probability and even in cases where other methods are ineffective. An exact diagnosis was made in 88% of the cases. This semi-quantitative method is also suitable for follow-up control since, in contrast to other techniques, even discrete liquorrheas can be identified considering that a subjectively observed cessation of flow may not be equated with an occlusion of the liquor fistula as shown by the 131I-hippuran swab test, which continues to be positive.", "contents": "[The diagnosis of cerebrospinal rhinorrhea with the 131I-hippuran swab test]. The report deals with the 131I-hippuran swab test and its effectiveness as compared with other methods (X-ray techniques, Clinistix) in 123 patients with rhinoliquorrhea. Results in 32 patients with rhinoliquorrhea are correlated with those of various methods of examination and with the surgical reports. In frontal and ethmoidal lesions and in those of the middle part of the face the liquorrheas can be demonstrated with a greater probability and even in cases where other methods are ineffective. An exact diagnosis was made in 88% of the cases. This semi-quantitative method is also suitable for follow-up control since, in contrast to other techniques, even discrete liquorrheas can be identified considering that a subjectively observed cessation of flow may not be equated with an occlusion of the liquor fistula as shown by the 131I-hippuran swab test, which continues to be positive."}
{"id": "PMID:471837", "title": "Outpatient oral anticoagulation: guidelines for long-term management.", "content": "The experience with outpatient anticoagulation services (ACSs) in two municipal teaching hospitals has been reviewed. These two ACSs have provided therapy with a major hemorrhagic morbidity of 4.1% based on patient treatment years and with no anticoagulation-related mortality. Their major goal has been to provide safe, efficient monitoring of anticoagulation control for the patients of a wide variety of primary care physicians in the hospital clinics. With careful patient selection and education, monitoring of the duration of therapy, and close communication among patient, primary care physician, and pharmacist, this goal has been achieved.", "contents": "Outpatient oral anticoagulation: guidelines for long-term management. The experience with outpatient anticoagulation services (ACSs) in two municipal teaching hospitals has been reviewed. These two ACSs have provided therapy with a major hemorrhagic morbidity of 4.1% based on patient treatment years and with no anticoagulation-related mortality. Their major goal has been to provide safe, efficient monitoring of anticoagulation control for the patients of a wide variety of primary care physicians in the hospital clinics. With careful patient selection and education, monitoring of the duration of therapy, and close communication among patient, primary care physician, and pharmacist, this goal has been achieved."}
{"id": "PMID:471839", "title": "The importance of significance and the significance of importance.", "content": "This article has attempted to illustrate the following principles of statistical significance. 1. An investigator must state the hypothesis to be tested beforehand, so that a null hypothesis can be constructed and validly tested using standard statistical techniques. 2. A statistically significant difference does not prove that a difference exists. It merely establishes that if no difference exists, there is only a small chance that the results obtained would have occurred. A small chance is traditionally and arbitrarily defined as 5% or less. However, even 5% may be too large when important decisions depend on the results. 3. Failure to establish a statistically significant difference does not prove that no difference exists. Chance alone or the small numbers used may have prevented the investigators from rejecting the null hypothesis. 4. A statistically significant difference may be too small to be clinically useful. Statistical significance can be demonstrated for even the most inconsequential of differences if the number of individuals used in the study is large enough. 5. The clinical usefulness of a statistically significant difference may be limited by the degree of overlap. This is reflected in the standard deviation around the averages. In answer to the questions posed at the beginning of this article: If it is statistically significant, it might be true. If it is statistically significant, it might be important. If it is statistically significant, it might be clinically useful.", "contents": "The importance of significance and the significance of importance. This article has attempted to illustrate the following principles of statistical significance. 1. An investigator must state the hypothesis to be tested beforehand, so that a null hypothesis can be constructed and validly tested using standard statistical techniques. 2. A statistically significant difference does not prove that a difference exists. It merely establishes that if no difference exists, there is only a small chance that the results obtained would have occurred. A small chance is traditionally and arbitrarily defined as 5% or less. However, even 5% may be too large when important decisions depend on the results. 3. Failure to establish a statistically significant difference does not prove that no difference exists. Chance alone or the small numbers used may have prevented the investigators from rejecting the null hypothesis. 4. A statistically significant difference may be too small to be clinically useful. Statistical significance can be demonstrated for even the most inconsequential of differences if the number of individuals used in the study is large enough. 5. The clinical usefulness of a statistically significant difference may be limited by the degree of overlap. This is reflected in the standard deviation around the averages. In answer to the questions posed at the beginning of this article: If it is statistically significant, it might be true. If it is statistically significant, it might be important. If it is statistically significant, it might be clinically useful."}
{"id": "PMID:471840", "title": "Genetic causes and workup of male and female infertility. 3. Details of the clinical evaluation.", "content": "In the evaluation of male and female infertility the history, family history, physical examination, and endocrine and gonadal functional evaluations are the most informative measures. The cause of the infertility is never found in some 17.5% of couples and in almost one fourth of males. In over one third of cases male infertility is attributed to varicocele. In 40% of women infertility can be attributed to ovulatory or cervical factors, uterotubal disease, endometriosis and other pelvic disease, or a combination of these factors. For couples with primary infertility the fertility rate after seven years is only 36%; in such cases the neonatal death rate, frequency of low birth weight, and incidence of major malformations are several time greater than in the normal population.", "contents": "Genetic causes and workup of male and female infertility. 3. Details of the clinical evaluation. In the evaluation of male and female infertility the history, family history, physical examination, and endocrine and gonadal functional evaluations are the most informative measures. The cause of the infertility is never found in some 17.5% of couples and in almost one fourth of males. In over one third of cases male infertility is attributed to varicocele. In 40% of women infertility can be attributed to ovulatory or cervical factors, uterotubal disease, endometriosis and other pelvic disease, or a combination of these factors. For couples with primary infertility the fertility rate after seven years is only 36%; in such cases the neonatal death rate, frequency of low birth weight, and incidence of major malformations are several time greater than in the normal population."}
{"id": "PMID:471841", "title": "Mania: the common symptom of several illnesses.", "content": "A clinical picture of mania can have many causes, including amphetamine or cocaine use, acute schizophrenia, manic-depressive illness, and viral encephalitis. Accurate and complete history--of both the present episode and past experiences--is most valuable in the differentiation, along with mental status examination, physical examination, and selected laboratory tests. It is advisable to withhold drug therapy, if possible, until the initial evaluation is completed, for it may obscure the diagnosis.", "contents": "Mania: the common symptom of several illnesses. A clinical picture of mania can have many causes, including amphetamine or cocaine use, acute schizophrenia, manic-depressive illness, and viral encephalitis. Accurate and complete history--of both the present episode and past experiences--is most valuable in the differentiation, along with mental status examination, physical examination, and selected laboratory tests. It is advisable to withhold drug therapy, if possible, until the initial evaluation is completed, for it may obscure the diagnosis."}
{"id": "PMID:471845", "title": "Nasal skin cancer:hazard to a uniquely exposed structure.", "content": "The nose is one of the most common sites of malignancy on the face, probably because of its unique position of exposure to environmental damage. By far the most common type of nasal tumor is basal cell carcinoma. Squamous cell carcinoma is less common but considerably more aggressive, with a tendency to fast growth and metastasis. Tumors of either type are more likely to invade easily and resist treatment if they are in certain \"danger zones.\" Nasal skin cancer in general has a relatively good prognosis, regardless of the type of treatment selected, ie, surgery, radiotherapy, cryotherapy, or electrodesiccation and curretage. However, tumors that are not eradicated have a long history of recurrences before proving fatal. When ablative surgery is performed, some type of reconstructive procedure is usually necessary.", "contents": "Nasal skin cancer:hazard to a uniquely exposed structure. The nose is one of the most common sites of malignancy on the face, probably because of its unique position of exposure to environmental damage. By far the most common type of nasal tumor is basal cell carcinoma. Squamous cell carcinoma is less common but considerably more aggressive, with a tendency to fast growth and metastasis. Tumors of either type are more likely to invade easily and resist treatment if they are in certain \"danger zones.\" Nasal skin cancer in general has a relatively good prognosis, regardless of the type of treatment selected, ie, surgery, radiotherapy, cryotherapy, or electrodesiccation and curretage. However, tumors that are not eradicated have a long history of recurrences before proving fatal. When ablative surgery is performed, some type of reconstructive procedure is usually necessary."}
{"id": "PMID:471846", "title": "A clinical look at the aging skin.", "content": "The aging Caucasian skin becomes yellow, dry, wrinkled and inelastic. Deterioration increases its susceptibility to malignant neoplasms, pruritus, and candidal infection. In addition, psychosomatic problems vascular disease, \"little strokes,\" and chronic malnutrition may precipitate or exacerbate skin disorders in the elderly. Treatment with \"strong\" medications should be taboo because of the fragility of senile skin. The risk of toxic reactions or of allergic sensitization is great. Because healing is retarded in old tissue, time is an indispensable component of successful treatment.", "contents": "A clinical look at the aging skin. The aging Caucasian skin becomes yellow, dry, wrinkled and inelastic. Deterioration increases its susceptibility to malignant neoplasms, pruritus, and candidal infection. In addition, psychosomatic problems vascular disease, \"little strokes,\" and chronic malnutrition may precipitate or exacerbate skin disorders in the elderly. Treatment with \"strong\" medications should be taboo because of the fragility of senile skin. The risk of toxic reactions or of allergic sensitization is great. Because healing is retarded in old tissue, time is an indispensable component of successful treatment."}
{"id": "PMID:471847", "title": "Unusual mucocutaneous infections in immunosuppressed patients with leukemia.", "content": "Patients with leukemia may become severely infected with usually nonpathogenic organisms and are also prey to infection in unusual sites and by unusual organisms.. Every organism isolated from such a patient must be considered a potential enemy, regardless of its pathogenic reputation, and evidence of infection must be sought.", "contents": "Unusual mucocutaneous infections in immunosuppressed patients with leukemia. Patients with leukemia may become severely infected with usually nonpathogenic organisms and are also prey to infection in unusual sites and by unusual organisms.. Every organism isolated from such a patient must be considered a potential enemy, regardless of its pathogenic reputation, and evidence of infection must be sought."}
{"id": "PMID:471848", "title": "Alcohol, caffeine, and nicotine as factors in pregnancy.", "content": "Use of alcohol, caffeine, and nicotine during pregnancy can have serious consequences for the fetus, such as low birth weight, congenital anomalies, or even death. Recent epidemiologic and animal studies indicate that these drugs may exert their effect by a number of metabolic and endocrine mechanisms, which are described here.", "contents": "Alcohol, caffeine, and nicotine as factors in pregnancy. Use of alcohol, caffeine, and nicotine during pregnancy can have serious consequences for the fetus, such as low birth weight, congenital anomalies, or even death. Recent epidemiologic and animal studies indicate that these drugs may exert their effect by a number of metabolic and endocrine mechanisms, which are described here."}
{"id": "PMID:471849", "title": "Who gets gallstones and why.", "content": "Gallstone formation is a heterogeneous disease for which supersaturation of bile with cholesterol and hemolysis of RBCs are major driving forces associated with initial formation and growth. Specific risk factors are superimposed on the gradually increasing prevalence of gallstones with age in most populations. Major risk factors associated with cholesterol gallstone formation are American Indian ancestry, female sex, obesity, and ingestion of lithogenic drugs, such as estrogen-containing preparations and clofibrate. Hemolysis and cirrhosis are risk factors for pigment stones.", "contents": "Who gets gallstones and why. Gallstone formation is a heterogeneous disease for which supersaturation of bile with cholesterol and hemolysis of RBCs are major driving forces associated with initial formation and growth. Specific risk factors are superimposed on the gradually increasing prevalence of gallstones with age in most populations. Major risk factors associated with cholesterol gallstone formation are American Indian ancestry, female sex, obesity, and ingestion of lithogenic drugs, such as estrogen-containing preparations and clofibrate. Hemolysis and cirrhosis are risk factors for pigment stones."}
{"id": "PMID:471850", "title": "Gram-negative bacteremia. Two cases in adults with chronic otitis media.", "content": "Chronic otitis media was the cause of septicemia in two hospitalized men. The organisms involved, K pneumoniae and B fragilis, are not usually associated with primary infections of the middle ear and would not have been traced to this site had proper cultures not been performed. In adults, the true incidence of bacteremia arising from the middle ear may be higher than suspected.", "contents": "Gram-negative bacteremia. Two cases in adults with chronic otitis media. Chronic otitis media was the cause of septicemia in two hospitalized men. The organisms involved, K pneumoniae and B fragilis, are not usually associated with primary infections of the middle ear and would not have been traced to this site had proper cultures not been performed. In adults, the true incidence of bacteremia arising from the middle ear may be higher than suspected."}
{"id": "PMID:471851", "title": "The fallacy of free extrapolation.", "content": "In determining the meaning of a study for other populations or situations, the investigator and the reviewer must be leery of extrapolating beyond the range of the data. They must beware of the ecological fallacies which can occur when population data are not linked to individual data. They must be conscious of the underlying assumptions to avoid drawing invalid conclusions about one population from valid studies on another. Finally, they must take into account the differences between the study population and the general population.", "contents": "The fallacy of free extrapolation. In determining the meaning of a study for other populations or situations, the investigator and the reviewer must be leery of extrapolating beyond the range of the data. They must beware of the ecological fallacies which can occur when population data are not linked to individual data. They must be conscious of the underlying assumptions to avoid drawing invalid conclusions about one population from valid studies on another. Finally, they must take into account the differences between the study population and the general population."}
{"id": "PMID:471852", "title": "Autoimmune hemolytic anemia. 2. Drug-induced type.", "content": "A variety of commonly prescribed drugs can produce a positive direct antiglobulin test, but the incidence of actual drug-induced hemolytic anemia is low. Of the four mechanisms which may be responsible, only one involves autoantibody production; the others include drug-RBC interaction of the hapten type, immune complex formation, and direct alteration of the RBC membrane.", "contents": "Autoimmune hemolytic anemia. 2. Drug-induced type. A variety of commonly prescribed drugs can produce a positive direct antiglobulin test, but the incidence of actual drug-induced hemolytic anemia is low. Of the four mechanisms which may be responsible, only one involves autoantibody production; the others include drug-RBC interaction of the hapten type, immune complex formation, and direct alteration of the RBC membrane."}
{"id": "PMID:471853", "title": "Pulmonary heart disease: pathophysiology, diagnostic signs, and therapy.", "content": "The anatomy and physiology of the pulmonary vasculature has been described with emphasis on several types of pulmonary hypertension and their pathophysiology. Treatment of pulmonary hypertension may be successful, especially when disease is related to emboli which respond to anticoagulant therapy. Relief of mitral valve obstruction is usually beneficial. Patients with abnormalities of blood PO2 and pH (especially in chronic obstructive lung disease) may be spared increased pulmonary pressure or may reverse existing pulmonary hypertension by giving up smoking, learning more efficient ventilatory methods, and when indicated, using supplemental oxygen, bronchodilators, digitalis, diuretics, or a combination of these.", "contents": "Pulmonary heart disease: pathophysiology, diagnostic signs, and therapy. The anatomy and physiology of the pulmonary vasculature has been described with emphasis on several types of pulmonary hypertension and their pathophysiology. Treatment of pulmonary hypertension may be successful, especially when disease is related to emboli which respond to anticoagulant therapy. Relief of mitral valve obstruction is usually beneficial. Patients with abnormalities of blood PO2 and pH (especially in chronic obstructive lung disease) may be spared increased pulmonary pressure or may reverse existing pulmonary hypertension by giving up smoking, learning more efficient ventilatory methods, and when indicated, using supplemental oxygen, bronchodilators, digitalis, diuretics, or a combination of these."}
{"id": "PMID:471854", "title": "Broncholithiasis: current concepts of an ancient disease.", "content": "Broncholithiasis, associated most frequently with tuberculosis and histoplasmosis, usually presents with acute onset of cough and hemoptysis. Visible stones are coughed up in fewer cases than was previously believed. The disease may be accompanied by obstructive symptoms, bronchiectasis, and occasional fistula formation into either the esophagus or the aorta. The prognosis of these patients is generally excellent; however, a significant number require surgery because of persistent symptoms or a complication of the disease.", "contents": "Broncholithiasis: current concepts of an ancient disease. Broncholithiasis, associated most frequently with tuberculosis and histoplasmosis, usually presents with acute onset of cough and hemoptysis. Visible stones are coughed up in fewer cases than was previously believed. The disease may be accompanied by obstructive symptoms, bronchiectasis, and occasional fistula formation into either the esophagus or the aorta. The prognosis of these patients is generally excellent; however, a significant number require surgery because of persistent symptoms or a complication of the disease."}
{"id": "PMID:471855", "title": "The atypical pneumonias: a diagnostic and therapeutic approach.", "content": "Mycoplasmal pneumonia, tularemic pneumonia, Q fever pneumonia, psittacosis, and Legionnaires' disease are the most frequently encountered treatable atypical pneumonias. Mycoplasmal pneumonia, the most common, is often accompanied by nonexudative pharyngitis, conjunctivitis, or otitis. The nonproductive cough is characteristic. Tularemic pneumonia is characterized by substernal chest pain, bloody pleural effusion, and bilateral hilar adenopathy. Although the clinical presentation is mild, roentgenographic findings are impressive. Q fever pneumonia resembles psittacosis but is less serious; it may be accompanied by subacute bacterial endocarditis, hepatitis, or both. Psittacosis is characterized by prominent headache, bloody sputum, and relative bradycardia. Tetracycline is the drug of choice for either. In Legionnaires' disease, pneumonia is accompanied by prominent extrapulmonary symptoms. The most important diagnostic clues include diarrhea and mental confusion. Relative bradycardia and laboratory abnormalities are also helpful. Erythromycin is the drug of choice unless doubt exists as to the diagnosis.", "contents": "The atypical pneumonias: a diagnostic and therapeutic approach. Mycoplasmal pneumonia, tularemic pneumonia, Q fever pneumonia, psittacosis, and Legionnaires' disease are the most frequently encountered treatable atypical pneumonias. Mycoplasmal pneumonia, the most common, is often accompanied by nonexudative pharyngitis, conjunctivitis, or otitis. The nonproductive cough is characteristic. Tularemic pneumonia is characterized by substernal chest pain, bloody pleural effusion, and bilateral hilar adenopathy. Although the clinical presentation is mild, roentgenographic findings are impressive. Q fever pneumonia resembles psittacosis but is less serious; it may be accompanied by subacute bacterial endocarditis, hepatitis, or both. Psittacosis is characterized by prominent headache, bloody sputum, and relative bradycardia. Tetracycline is the drug of choice for either. In Legionnaires' disease, pneumonia is accompanied by prominent extrapulmonary symptoms. The most important diagnostic clues include diarrhea and mental confusion. Relative bradycardia and laboratory abnormalities are also helpful. Erythromycin is the drug of choice unless doubt exists as to the diagnosis."}
{"id": "PMID:471856", "title": "Effect of domperidone on gastric emptying and secretion.", "content": "Domperidone (5 mg i.v.) was given singly or together with apomorphine (0.25 mg i.v.) to healthy volunteers. The gastric emptying rate of a test meal of 750 ml of water was then measured using a dye dilution and double sampling technique. The effect of domperidone on gastric secretion was evaluated in patients with duodenal ulcer who received domperidone (5 mg i.v.) prior to continuous aspiration of the stomach contents for 120 minutes. Domperidone markedly increased the rate of emptying of the test meal. Following apomorphine the gastric emptying rate decreased and domperidone antagonized this slowing effect. The composition of gastric juice was not affected by an intravenous dose of domperidone. Domperidone appears to modulate gastric contractile activity without affecting gastric secretion.", "contents": "Effect of domperidone on gastric emptying and secretion. Domperidone (5 mg i.v.) was given singly or together with apomorphine (0.25 mg i.v.) to healthy volunteers. The gastric emptying rate of a test meal of 750 ml of water was then measured using a dye dilution and double sampling technique. The effect of domperidone on gastric secretion was evaluated in patients with duodenal ulcer who received domperidone (5 mg i.v.) prior to continuous aspiration of the stomach contents for 120 minutes. Domperidone markedly increased the rate of emptying of the test meal. Following apomorphine the gastric emptying rate decreased and domperidone antagonized this slowing effect. The composition of gastric juice was not affected by an intravenous dose of domperidone. Domperidone appears to modulate gastric contractile activity without affecting gastric secretion."}
{"id": "PMID:471857", "title": "The effect of domperidone on pyloric activity in dog and in man.", "content": "In a study in dogs using ultrasonic transducers to measure degree of pyloric dilatation, it was found that i.v. domperidone (0.35 and 0.7 mg/kg) significantly increased the diameter of the pyloric lumen without affecting the frequency of pyloric relaxations. A subsequent study involving 59 patients referred for upper digestive tract endoscopy, confirmed this increase in diameter of the pylorus which indicates a potential use of domperidone (either i.v. or oral) in the treatment of some forms of dyspepsia characterized by delayed gastric emptying.", "contents": "The effect of domperidone on pyloric activity in dog and in man. In a study in dogs using ultrasonic transducers to measure degree of pyloric dilatation, it was found that i.v. domperidone (0.35 and 0.7 mg/kg) significantly increased the diameter of the pyloric lumen without affecting the frequency of pyloric relaxations. A subsequent study involving 59 patients referred for upper digestive tract endoscopy, confirmed this increase in diameter of the pylorus which indicates a potential use of domperidone (either i.v. or oral) in the treatment of some forms of dyspepsia characterized by delayed gastric emptying."}
{"id": "PMID:471858", "title": "The effect of darkness on vitamin D in adults.", "content": "Circulating 25-hydroxy vitamin D measurements were made on Caucasians in the Antarctic. Following a period of 3 months' complete darkness there was a significant fall in 25-hydroxy vitamin D values. These results emphasize the importance of u.v. light on skin synthesis of cholecalciferol and support the view that the geriatric and immigrant population should be adequately exposed to sunlight to prevent vitamin D deficiency.", "contents": "The effect of darkness on vitamin D in adults. Circulating 25-hydroxy vitamin D measurements were made on Caucasians in the Antarctic. Following a period of 3 months' complete darkness there was a significant fall in 25-hydroxy vitamin D values. These results emphasize the importance of u.v. light on skin synthesis of cholecalciferol and support the view that the geriatric and immigrant population should be adequately exposed to sunlight to prevent vitamin D deficiency."}
{"id": "PMID:471859", "title": "The clinical pathology of heart failure and atrial fibrillation in old age.", "content": "The factors associated with the development of heart failure and of atrial fibrillation in elderly patients were studied in a prospective clinico-pathological series of 171 cases. Multiple logistic analyses allowed the contributions of multiple factors to be assessed simultaneously. Senile cardiac amyloidosis, ischaemic heart disease and atrial fibrillation were significantly associated with heart failure and had additive effects. Senile cardiac amyloidosis, ischaemic heart disease, mitral valve ballooning and higher age were significantly associated with atrial fibrillation but their additive effects were modified by significant interactions between ischaemic heart disease and mitral valve ballooning (whose effects were multiplicative) and senile cardiac amyloidosis and age (the age effect being confined to those without amyloidosis).", "contents": "The clinical pathology of heart failure and atrial fibrillation in old age. The factors associated with the development of heart failure and of atrial fibrillation in elderly patients were studied in a prospective clinico-pathological series of 171 cases. Multiple logistic analyses allowed the contributions of multiple factors to be assessed simultaneously. Senile cardiac amyloidosis, ischaemic heart disease and atrial fibrillation were significantly associated with heart failure and had additive effects. Senile cardiac amyloidosis, ischaemic heart disease, mitral valve ballooning and higher age were significantly associated with atrial fibrillation but their additive effects were modified by significant interactions between ischaemic heart disease and mitral valve ballooning (whose effects were multiplicative) and senile cardiac amyloidosis and age (the age effect being confined to those without amyloidosis)."}
{"id": "PMID:471860", "title": "Comparison of two coronary prognostic indices.", "content": "Two coronary prognostic indices for patients treated in a coronary care unit, namely the Norris Index and the Chapman-Gray Index were compared. Both were found to be reliable but the Chapman-Gray Index with its ready reckoner was more convenient and was subject to fewer subjective data.", "contents": "Comparison of two coronary prognostic indices. Two coronary prognostic indices for patients treated in a coronary care unit, namely the Norris Index and the Chapman-Gray Index were compared. Both were found to be reliable but the Chapman-Gray Index with its ready reckoner was more convenient and was subject to fewer subjective data."}
{"id": "PMID:471861", "title": "The incidence of postoperative pulmonary perfusion defects.", "content": "The incidence of postoperative thromboembolism was studied using radioisotope lung perfusion and ventilation scanning. 26% of patients had defects on their pre-operative scans and 8% developed new unmatched perfusion defects postoperatively, indicating possible pulmonary emboli. This latter figure was much lower than some previously recorded results and was similar in 2 groups of patients--one receiving dextran 70 prophylaxis during operation and the other a control group who received saline.", "contents": "The incidence of postoperative pulmonary perfusion defects. The incidence of postoperative thromboembolism was studied using radioisotope lung perfusion and ventilation scanning. 26% of patients had defects on their pre-operative scans and 8% developed new unmatched perfusion defects postoperatively, indicating possible pulmonary emboli. This latter figure was much lower than some previously recorded results and was similar in 2 groups of patients--one receiving dextran 70 prophylaxis during operation and the other a control group who received saline."}
{"id": "PMID:471862", "title": "Hip fracture after hemiplegia.", "content": "In a series of 57 hemiplegic patients who subsequently fractured their hips, it was found that hip fracture occurred significantly more often on the hemiplegic side. Hip fracture was equally common in right- and left-sided hemiplegia, and often occurred within one year of the stroke. Two factors seem to be important in the genesis of hip fractures in hemiplegic patients: the tendency of stroke patients to fall to the affected side as a result of impaired locomotor function, and the development of disuse osteoporosis in the hemiplegic limb.", "contents": "Hip fracture after hemiplegia. In a series of 57 hemiplegic patients who subsequently fractured their hips, it was found that hip fracture occurred significantly more often on the hemiplegic side. Hip fracture was equally common in right- and left-sided hemiplegia, and often occurred within one year of the stroke. Two factors seem to be important in the genesis of hip fractures in hemiplegic patients: the tendency of stroke patients to fall to the affected side as a result of impaired locomotor function, and the development of disuse osteoporosis in the hemiplegic limb."}
{"id": "PMID:471863", "title": "Idiopathic pulmonary haemosiderosis: report of two cases and review of the literature.", "content": "Idiopathic pulmonary haemosiderosis (IPH) is a rare disorder affecting mainly children and adolescents, and is usually fatal. However, there is increasing evidence that the aetiology of this condition is immunological, and that immunosuppressives may prolong remission. Two cases are reported, one of which has the atypical feature of a malabsorption syndrome. This has not been previously reported. The literature is reviewed and current ideas on the postulated immunological basis to IPH are discussed.", "contents": "Idiopathic pulmonary haemosiderosis: report of two cases and review of the literature. Idiopathic pulmonary haemosiderosis (IPH) is a rare disorder affecting mainly children and adolescents, and is usually fatal. However, there is increasing evidence that the aetiology of this condition is immunological, and that immunosuppressives may prolong remission. Two cases are reported, one of which has the atypical feature of a malabsorption syndrome. This has not been previously reported. The literature is reviewed and current ideas on the postulated immunological basis to IPH are discussed."}
{"id": "PMID:471864", "title": "Pneumopericardium and pneumomediastinum complicating endotracheal intubation.", "content": "Pneumopericardium and pneumomediastinum have been described as complications of endotracheal intubation and assisted ventilation in neonates and children. Here the occurrence of these complications in an adult is described and the possible mechanism discussed.", "contents": "Pneumopericardium and pneumomediastinum complicating endotracheal intubation. Pneumopericardium and pneumomediastinum have been described as complications of endotracheal intubation and assisted ventilation in neonates and children. Here the occurrence of these complications in an adult is described and the possible mechanism discussed."}
{"id": "PMID:471865", "title": "Colonic tuberculosis.", "content": "A case of tuberculosis of the colon is described. Attention is drawn to the similarity between this and other inflammatory diseases of the bowel. Colonic tuberculosis is now a rarity even where mycobacterial infection is endemic, and may mimic other inflammatory diseases of the bowel with important consequences for treatment and prognosis. Such a case is now described.", "contents": "Colonic tuberculosis. A case of tuberculosis of the colon is described. Attention is drawn to the similarity between this and other inflammatory diseases of the bowel. Colonic tuberculosis is now a rarity even where mycobacterial infection is endemic, and may mimic other inflammatory diseases of the bowel with important consequences for treatment and prognosis. Such a case is now described."}
{"id": "PMID:471866", "title": "Levamisole in the treatment of cyclical neutropenia.", "content": "A child with cyclical neutropenia was observed before and during treatment with levamisole. Marked clinical improvement occurred while on levamisole. Deterioration occurred after stopping treatment. The improvement was not associated with changes in the neutrophil or monocyte counts, nor did tests of phagocytic cell function convincingly demonstrate improvement while the child was on treatment.", "contents": "Levamisole in the treatment of cyclical neutropenia. A child with cyclical neutropenia was observed before and during treatment with levamisole. Marked clinical improvement occurred while on levamisole. Deterioration occurred after stopping treatment. The improvement was not associated with changes in the neutrophil or monocyte counts, nor did tests of phagocytic cell function convincingly demonstrate improvement while the child was on treatment."}
{"id": "PMID:471867", "title": "Epsilon aminocaproic acid (EACA) myopathy.", "content": "Two female patients developed a severe, painful proximal myopathy after taking 18--30 g of epsilon-aminocaproic acid daily for 5 weeks. Marked elevations of serum aminotransferases, creatine kinase and aldolase levels were found and the first patient had electromyographic and muscle biopsy changes of an acute monophasic, necrotising myopathy at the height of the illness. Resolution occurred in both cases on stopping the drug and the second patient had no electromyographic or muscle biopsy abnormalities 3 weeks later. Only 2 recognized cases of the condition have been reported previously but a review of the literature revealed several other possible examples.", "contents": "Epsilon aminocaproic acid (EACA) myopathy. Two female patients developed a severe, painful proximal myopathy after taking 18--30 g of epsilon-aminocaproic acid daily for 5 weeks. Marked elevations of serum aminotransferases, creatine kinase and aldolase levels were found and the first patient had electromyographic and muscle biopsy changes of an acute monophasic, necrotising myopathy at the height of the illness. Resolution occurred in both cases on stopping the drug and the second patient had no electromyographic or muscle biopsy abnormalities 3 weeks later. Only 2 recognized cases of the condition have been reported previously but a review of the literature revealed several other possible examples."}
{"id": "PMID:471868", "title": "Central circulatory responses in normotensive and hypertensive pregnancy.", "content": "Haemodynamic responses in normotensive pregnancy in the antenatal phase show that cardiac output is elevated by the end of the first trimester, and remains elevated throughout pregnancy, although it may fall very slightly at the end of the third trimester of pregnancy. Heart rate remains virtually static throughout pregnancy, although it may fall very slightly at the end of the third trimester of pregnancy. Arterial blood pressure remains virtually unaltered. During labour in patients with traditional anaesthesia, cardiac output is elevated by 40% overall at the end of the labour, and this rise may be as much as 60% in the immediate post-partum period. Patients with epidural analgesia show no overall rise in cardiac output throughout labour. Following Caesarean section there may be massive rises in cardiac output. In patients who become hypertensive as a result of pregnancy, there are marked individual patterns. These different groups show patients with elevated levels of cardiac output, patients with a pure elevation of systemic vascular resistance, and a third group in which there is elevation of both resistance and output. It seems certain that different syndromes are occurring, the theoretical explanations for which are discussed.", "contents": "Central circulatory responses in normotensive and hypertensive pregnancy. Haemodynamic responses in normotensive pregnancy in the antenatal phase show that cardiac output is elevated by the end of the first trimester, and remains elevated throughout pregnancy, although it may fall very slightly at the end of the third trimester of pregnancy. Heart rate remains virtually static throughout pregnancy, although it may fall very slightly at the end of the third trimester of pregnancy. Arterial blood pressure remains virtually unaltered. During labour in patients with traditional anaesthesia, cardiac output is elevated by 40% overall at the end of the labour, and this rise may be as much as 60% in the immediate post-partum period. Patients with epidural analgesia show no overall rise in cardiac output throughout labour. Following Caesarean section there may be massive rises in cardiac output. In patients who become hypertensive as a result of pregnancy, there are marked individual patterns. These different groups show patients with elevated levels of cardiac output, patients with a pure elevation of systemic vascular resistance, and a third group in which there is elevation of both resistance and output. It seems certain that different syndromes are occurring, the theoretical explanations for which are discussed."}
{"id": "PMID:471869", "title": "Cardiac disease in pregnancy.", "content": "The problems encountered in the management of pregnant patients with valvular, congenital and ischaemic heart disease are described with the help of illustrative cases. The increased incidence of cardiac dysrhythmias in pregnancy is also discussed.", "contents": "Cardiac disease in pregnancy. The problems encountered in the management of pregnant patients with valvular, congenital and ischaemic heart disease are described with the help of illustrative cases. The increased incidence of cardiac dysrhythmias in pregnancy is also discussed."}
{"id": "PMID:471870", "title": "The management of thyroid disease in pregnancy.", "content": "The management of hyperthyroidism and hypothyroidism in pregnancy is discussed and illustrated with appropriate cases. The dangers of the usage of high doses of antithyroid drugs and of propranolol are described.", "contents": "The management of thyroid disease in pregnancy. The management of hyperthyroidism and hypothyroidism in pregnancy is discussed and illustrated with appropriate cases. The dangers of the usage of high doses of antithyroid drugs and of propranolol are described."}
{"id": "PMID:471871", "title": "Clinical aspects of diabetes in pregnancy.", "content": "The introduction of insulin produced a rapid improvement in the maternal prognosis in pregnancy associated with diabetes mellitus. Within years, maternal mortality fell from 50% and has become a rarity. Improvement in fetal survival was less dramatic until the work of Pedersen (1952); Peel and Oakley (1950) and White (1949) demonstrated that with determined antenatal control of diabetes the fetal outcome was greatly improved. Further improvement in the care of the fetus of the pregnant diabetic with fetal monitoring, both ante- and intra-partum, together with modern neonatal expertise, had brought the perinatal and neonatal morbidity and mortality for this group of patients close to that of the non-diabetic. An account is given of the author's present day practice in this group of patients.", "contents": "Clinical aspects of diabetes in pregnancy. The introduction of insulin produced a rapid improvement in the maternal prognosis in pregnancy associated with diabetes mellitus. Within years, maternal mortality fell from 50% and has become a rarity. Improvement in fetal survival was less dramatic until the work of Pedersen (1952); Peel and Oakley (1950) and White (1949) demonstrated that with determined antenatal control of diabetes the fetal outcome was greatly improved. Further improvement in the care of the fetus of the pregnant diabetic with fetal monitoring, both ante- and intra-partum, together with modern neonatal expertise, had brought the perinatal and neonatal morbidity and mortality for this group of patients close to that of the non-diabetic. An account is given of the author's present day practice in this group of patients."}
{"id": "PMID:471872", "title": "[Pulmonary mucormycoses (author's transl)].", "content": "Mucormycoses are rare, acute infections, most often fatal, occurring usually in a host with decreased immunity. The agents are ubiquitous fungi belonging to the Mucoraceae family, an ordinary saprophyte becoming pathogenous in patients with a severe disease (malignant hemopathy, diabetes) or treated by immunosuppressive drugs. Pulmonary localizations remained exceptional (128 cases found in the literature). The observations reported draws its originality from: -- the etiological circumstances: an aged patient with no hemopathy or diabetes, without immunosuppressive treatment but undergoing a prolonged antibiotic therapy; -- the clinical signs: discovery on X-ray of excavated infiltrates in a background of a severe infection with a very poor physical condition; -- the diagnosis criteria with the demonstration of the responsible mucor in the sputum, the endobronchial aspiration and transparietal puncture; -- the great efficiency of Amphotericin B at rather small doses. A review of the literature underlines the exceptional character of the cure.", "contents": "[Pulmonary mucormycoses (author's transl)]. Mucormycoses are rare, acute infections, most often fatal, occurring usually in a host with decreased immunity. The agents are ubiquitous fungi belonging to the Mucoraceae family, an ordinary saprophyte becoming pathogenous in patients with a severe disease (malignant hemopathy, diabetes) or treated by immunosuppressive drugs. Pulmonary localizations remained exceptional (128 cases found in the literature). The observations reported draws its originality from: -- the etiological circumstances: an aged patient with no hemopathy or diabetes, without immunosuppressive treatment but undergoing a prolonged antibiotic therapy; -- the clinical signs: discovery on X-ray of excavated infiltrates in a background of a severe infection with a very poor physical condition; -- the diagnosis criteria with the demonstration of the responsible mucor in the sputum, the endobronchial aspiration and transparietal puncture; -- the great efficiency of Amphotericin B at rather small doses. A review of the literature underlines the exceptional character of the cure."}
{"id": "PMID:471873", "title": "[Respiratory signs linked to passive inhalation of tobacco smoke in infants (author's transl)].", "content": "Thirty observations done on infants formed the base of a review of the literature on the epidemiology, the clinical signs and the physiopathological mechanisms of passive smoking (PS). The main signal was a chronic cough but in infants under 12 months, PS also provoked, as well, an \"asthmatoid bronchitis\", progressing relentlessly since the coming out of maternity ward, in relation with the maternal tobacco addiction. In these patients, examinations failed to disclose the different causes of recurring respiratory infections. Among the granulocytic tests, only the spontaneous migration of neutrophil polykaryocytes in the absence of chemotactic stimulus was significantly lowered, by comparison with the controls. The authors did a review on epidemiological surveys done to this day; they revealed a direct relationship between the parent's addiction to tobacco and the frequency of recurring respiratory disorders. It was particularly evident when the mother smoked and the child was under 12 months. At this age it was independant from the infection eventually transmitted by the parent's cough. The constituants of tobacco smoke probably have a depressive action over the regional or perhaps the general means of immunitary defense as it is suggested by the decrease of leukocyte migration noted in parents with PS. It is therefore necessary in our country to define the true risks of PS in infants thanks to well organized epidemiological surveys and to obtain the means of an efficient preventive policy.", "contents": "[Respiratory signs linked to passive inhalation of tobacco smoke in infants (author's transl)]. Thirty observations done on infants formed the base of a review of the literature on the epidemiology, the clinical signs and the physiopathological mechanisms of passive smoking (PS). The main signal was a chronic cough but in infants under 12 months, PS also provoked, as well, an \"asthmatoid bronchitis\", progressing relentlessly since the coming out of maternity ward, in relation with the maternal tobacco addiction. In these patients, examinations failed to disclose the different causes of recurring respiratory infections. Among the granulocytic tests, only the spontaneous migration of neutrophil polykaryocytes in the absence of chemotactic stimulus was significantly lowered, by comparison with the controls. The authors did a review on epidemiological surveys done to this day; they revealed a direct relationship between the parent's addiction to tobacco and the frequency of recurring respiratory disorders. It was particularly evident when the mother smoked and the child was under 12 months. At this age it was independant from the infection eventually transmitted by the parent's cough. The constituants of tobacco smoke probably have a depressive action over the regional or perhaps the general means of immunitary defense as it is suggested by the decrease of leukocyte migration noted in parents with PS. It is therefore necessary in our country to define the true risks of PS in infants thanks to well organized epidemiological surveys and to obtain the means of an efficient preventive policy."}
{"id": "PMID:471874", "title": "[Prevention of thrombo-embolic accidents after pneumonectomy (author's transl)].", "content": "The authors analyzed the progress in the prevention of thrombo-embolic risk in 550 pneumonectomies divided chronologically in 4 groups : without anticoagulant treatment, with post-operative anti-vitamin K, with post-operative calcium heparinate, with pre and post-operative calcium heparinate. This study revealed the necessity of a pre-operative systematic preventive treatment : systematic, as there is no biological reason enabling the prevention of thrombosis risk. Pre-operative, because venous thrombosis and pulmonary embolism can occur very early.", "contents": "[Prevention of thrombo-embolic accidents after pneumonectomy (author's transl)]. The authors analyzed the progress in the prevention of thrombo-embolic risk in 550 pneumonectomies divided chronologically in 4 groups : without anticoagulant treatment, with post-operative anti-vitamin K, with post-operative calcium heparinate, with pre and post-operative calcium heparinate. This study revealed the necessity of a pre-operative systematic preventive treatment : systematic, as there is no biological reason enabling the prevention of thrombosis risk. Pre-operative, because venous thrombosis and pulmonary embolism can occur very early."}
{"id": "PMID:471875", "title": "[Dystrophic hyperlucent lung (author's transl)].", "content": "Emphysematous bullae isolated on a healthy lung and primitive progressive dystrophic emphysema all belong to the dystrophic hyperlucent lung and are surgical cases. Having recalled the excellent prognosis of isolated bullae, the authors analyzed the surgical indications in the progressive dystrophic emphysema concerning 66 observations of which 52 cases were operated. They showed that surgery provided many improvements and that making a pleural adhesion during the intervention limited the bullous evolution of the disease. Results plead in favour of early interventions and wider indications.", "contents": "[Dystrophic hyperlucent lung (author's transl)]. Emphysematous bullae isolated on a healthy lung and primitive progressive dystrophic emphysema all belong to the dystrophic hyperlucent lung and are surgical cases. Having recalled the excellent prognosis of isolated bullae, the authors analyzed the surgical indications in the progressive dystrophic emphysema concerning 66 observations of which 52 cases were operated. They showed that surgery provided many improvements and that making a pleural adhesion during the intervention limited the bullous evolution of the disease. Results plead in favour of early interventions and wider indications."}
{"id": "PMID:471876", "title": "[Value of vascular tests in the diagnosis and therapeutical indications of pulmonary embolism on a pneumological background (author's transl)].", "content": "The diagnosis of mislaying forms of pulmonary embolism, where even angiography was not pathognomonic, induced the use of a phlebography on lower limbs in patients where this diagnosis was suspected. The revelation of a distal thrombo-phlebitis and even more so an iliocaval one, becomes a major value when the clinical and paraclinical data remained arguable. The comparative study of diagnostic usual clinical and paraclinical elements (radiographies, E.C.G., gasometries and scintigraphies) confirmed, in 22 patients, the value of phlebography. On a pneumological background, it is useful to perform this examination, most of the time : a) on patients over 55, when a cardiorespiratory decompensation remained unexplained in a patient with chronic respiratory insufficiency, or even an unproved hemoptysis; b) but mostly before 55, in case of painful pneumopathy above all if it is bilateral and recurring, or in case of paroxysmal bronchospasm without atopic ground, when a belated asthma could be thought of. Finally vascular tests induced the fitting in situ of a clamp or \"umbrella\" in the vena cava in 7 patients out of 22.", "contents": "[Value of vascular tests in the diagnosis and therapeutical indications of pulmonary embolism on a pneumological background (author's transl)]. The diagnosis of mislaying forms of pulmonary embolism, where even angiography was not pathognomonic, induced the use of a phlebography on lower limbs in patients where this diagnosis was suspected. The revelation of a distal thrombo-phlebitis and even more so an iliocaval one, becomes a major value when the clinical and paraclinical data remained arguable. The comparative study of diagnostic usual clinical and paraclinical elements (radiographies, E.C.G., gasometries and scintigraphies) confirmed, in 22 patients, the value of phlebography. On a pneumological background, it is useful to perform this examination, most of the time : a) on patients over 55, when a cardiorespiratory decompensation remained unexplained in a patient with chronic respiratory insufficiency, or even an unproved hemoptysis; b) but mostly before 55, in case of painful pneumopathy above all if it is bilateral and recurring, or in case of paroxysmal bronchospasm without atopic ground, when a belated asthma could be thought of. Finally vascular tests induced the fitting in situ of a clamp or \"umbrella\" in the vena cava in 7 patients out of 22."}
{"id": "PMID:471877", "title": "[Broncho-pulmonary amylosis (about two cases) (author's transl)].", "content": "The authors reported two recent cases of broncho-pulmonary amylosis with monoclonal gammapathy. The first observation concerned a diffuse amylosis characterized by a bilateral pulmonary infiltration with pleural effusions and secretion of IgGlambda with  a Bence Jones proteinuria. Pleural and pulmonary biopsies will provide the proof of amylosis. Evolution will rapidly be fatal in case of renal insufficiency. Post-mortem sampling will confirm the amyloid involvement (muscle, liver, kidney). The second observation described a localized amylosis developed in contact with a mediastino-pulmonary lymphoplasmocytic lymphoma with tumoral secretion of IgMlambda. Although pneumonectomy brought out the disappearance of the abnormal paraportein, death occurred within a few months, linked either to the spreading of the lymphomatous process or to the onset of a bacillary surinfection. The authors then recalled the main clinical and diagnostic data of the broncho-pulmonary amylosis. They underlined the importance of GLENNER'S work who, by proving the immunoglobulinic nature of amyloid, explained that in both cases, amylosis appeared as the consequences of an abnormal immunoglobulins synthesis.", "contents": "[Broncho-pulmonary amylosis (about two cases) (author's transl)]. The authors reported two recent cases of broncho-pulmonary amylosis with monoclonal gammapathy. The first observation concerned a diffuse amylosis characterized by a bilateral pulmonary infiltration with pleural effusions and secretion of IgGlambda with  a Bence Jones proteinuria. Pleural and pulmonary biopsies will provide the proof of amylosis. Evolution will rapidly be fatal in case of renal insufficiency. Post-mortem sampling will confirm the amyloid involvement (muscle, liver, kidney). The second observation described a localized amylosis developed in contact with a mediastino-pulmonary lymphoplasmocytic lymphoma with tumoral secretion of IgMlambda. Although pneumonectomy brought out the disappearance of the abnormal paraportein, death occurred within a few months, linked either to the spreading of the lymphomatous process or to the onset of a bacillary surinfection. The authors then recalled the main clinical and diagnostic data of the broncho-pulmonary amylosis. They underlined the importance of GLENNER'S work who, by proving the immunoglobulinic nature of amyloid, explained that in both cases, amylosis appeared as the consequences of an abnormal immunoglobulins synthesis."}
{"id": "PMID:471878", "title": "The amino acid and energy requirements of broiler breeder hens.", "content": "Three trials, involving 1000 broiler breeder hens each, were conducted under rather uniform conditions in order to test the applicability of two models for calculating the amino acid requirements of broiler breeders, and to evaluate their energy requirements. The birds fed diets based on Model A (which assumes that all of the egg white and shell membrane proteins are synthesized during albumen formation and secretion at the expense of tissue proteins) produced at a rate greater than the planned target, whereas Model B (which assumes that only ovoglycoproteins and shell membranes are thus produced) supported a production close to the anticipated performance. This indicates the validity of Model B, developed for egg layers, to predict the requirements of the most limiting amino also for broiler breeder hens. The resulting practical diets contained considerably lower protein levels than customary. According to Model B, minimal amino acid requirements for broiler breeder hens producing a daily egg mass of 52.7 g. having a body weight of 3.5 kg and gaining weight at a rate of 4 g/day, calculated to be as follows (g/hen/day): methionine, .57; sulfur amino acids, .83; lysine, .76; arginine, 1.11; threonine, .72; valine, .92; isoleucine, .85; leucine, 1.25; tryptophane, .19; histidine,", "contents": "The amino acid and energy requirements of broiler breeder hens. Three trials, involving 1000 broiler breeder hens each, were conducted under rather uniform conditions in order to test the applicability of two models for calculating the amino acid requirements of broiler breeders, and to evaluate their energy requirements. The birds fed diets based on Model A (which assumes that all of the egg white and shell membrane proteins are synthesized during albumen formation and secretion at the expense of tissue proteins) produced at a rate greater than the planned target, whereas Model B (which assumes that only ovoglycoproteins and shell membranes are thus produced) supported a production close to the anticipated performance. This indicates the validity of Model B, developed for egg layers, to predict the requirements of the most limiting amino also for broiler breeder hens. The resulting practical diets contained considerably lower protein levels than customary. According to Model B, minimal amino acid requirements for broiler breeder hens producing a daily egg mass of 52.7 g. having a body weight of 3.5 kg and gaining weight at a rate of 4 g/day, calculated to be as follows (g/hen/day): methionine, .57; sulfur amino acids, .83; lysine, .76; arginine, 1.11; threonine, .72; valine, .92; isoleucine, .85; leucine, 1.25; tryptophane, .19; histidine,"}
{"id": "PMID:471879", "title": "Interrelationship between dietary pyridoxine and free plasma amino acids in chicks.", "content": "The interrelationships between dietary pyridoxine and free plasma amino acids in young chicks were studied with two different diets. Chicks fed isolated soybean protein supplemented with less than 1.0 mg pyridoxine-HCl/kg diet had reduced body weight gain and feed consumption and a higher mortality rate (19%) than those fed 1 or 5 mg pyridoxine-HCl/kg. A casein diet required greater supplementation (2.0 mg pyridoxine/kg) to prevent reduced body weight gain and depressed feed intake. The concentrations of free plasma taurine, threonine, and serine were significantly lower in chicks fed the isolated soybean protein diet supplemented with less than 1.0 mg pyridoxine-HCl/kg, while those of isoleucine, valine, glycine, and ornithine were significantly higher than in chicks fed greater levels of pyridoxine. Supplementation of casein diets with less than 2.0 mg resulted in a significant accumulation of threonine, glycine, cystathionine, and ornithine. Valine was significant higher in chicks fed casein supplemented with only .6 mg pyridoxine-HCl/kg. Feeding isolated soybean protein or casein diets without any pyridoxine supplementation resulted in the dealth of 95% and 100% of the chicks, respectively. Pair feeding a group fed the isolated soybean protein diet supplemented with 1.0 mg to a group receiving .5 mg pyridoxine-HCl/kg resulted in a similar body weight gain and concentration of amino acids in the plasma.", "contents": "Interrelationship between dietary pyridoxine and free plasma amino acids in chicks. The interrelationships between dietary pyridoxine and free plasma amino acids in young chicks were studied with two different diets. Chicks fed isolated soybean protein supplemented with less than 1.0 mg pyridoxine-HCl/kg diet had reduced body weight gain and feed consumption and a higher mortality rate (19%) than those fed 1 or 5 mg pyridoxine-HCl/kg. A casein diet required greater supplementation (2.0 mg pyridoxine/kg) to prevent reduced body weight gain and depressed feed intake. The concentrations of free plasma taurine, threonine, and serine were significantly lower in chicks fed the isolated soybean protein diet supplemented with less than 1.0 mg pyridoxine-HCl/kg, while those of isoleucine, valine, glycine, and ornithine were significantly higher than in chicks fed greater levels of pyridoxine. Supplementation of casein diets with less than 2.0 mg resulted in a significant accumulation of threonine, glycine, cystathionine, and ornithine. Valine was significant higher in chicks fed casein supplemented with only .6 mg pyridoxine-HCl/kg. Feeding isolated soybean protein or casein diets without any pyridoxine supplementation resulted in the dealth of 95% and 100% of the chicks, respectively. Pair feeding a group fed the isolated soybean protein diet supplemented with 1.0 mg to a group receiving .5 mg pyridoxine-HCl/kg resulted in a similar body weight gain and concentration of amino acids in the plasma."}
{"id": "PMID:471880", "title": "Studies on duck nutrition. II. Calcium and phosphorus requirements of mule ducklings.", "content": "Experiments were conducted to determine calcium and phosphorus requirements of mule ducklings. Two-day-old, straight-run mule ducklings were fed diets containing graded levels of calcium or phosphorus for three weeks. Weight gain, feed consumption, tibia ash content, and levels of serum calcium and phosphorus were used as criteria for the determination of the optimum dietary calcium and phosphorus levels required by the ducklings. The minimum calcium requirements were .48% and .58%, respectively, for maximum growth and optimum tibia ash content as determined by regression analysis of body weight gain and tibia ash content against calcium level in the diet. The minimum available phosphorus requirements were .26% and .34%, respectively, for maximum body weight gain and optimum tibia ash content as determined by the same method.", "contents": "Studies on duck nutrition. II. Calcium and phosphorus requirements of mule ducklings. Experiments were conducted to determine calcium and phosphorus requirements of mule ducklings. Two-day-old, straight-run mule ducklings were fed diets containing graded levels of calcium or phosphorus for three weeks. Weight gain, feed consumption, tibia ash content, and levels of serum calcium and phosphorus were used as criteria for the determination of the optimum dietary calcium and phosphorus levels required by the ducklings. The minimum calcium requirements were .48% and .58%, respectively, for maximum growth and optimum tibia ash content as determined by regression analysis of body weight gain and tibia ash content against calcium level in the diet. The minimum available phosphorus requirements were .26% and .34%, respectively, for maximum body weight gain and optimum tibia ash content as determined by the same method."}
{"id": "PMID:471881", "title": "Phosphate and heat treatments to control Salmonella and reduce spoilage and rancidity on broiler carcasses.", "content": "Broiler chicken carcasses were inoculated with about 300 cells of a naladixic acid resistant strain of Salmonella typhimurium, then treated for 3 min with highly agitated water with or without 6% phosphate added, at 65, 70, 75, 80, 85, or 90 C. Other broiler carcasses were inoculated with about 60 cells, then similarly treated at 65 or 70 C. All carcasses were chilled after the hot water treatment in a system simulating commerical chilling. When inoculum was 300 cells, at least 5% of the carcasses remained contaminated with Salmonella, even when hot water treatment was at 90 C. When inoculum was 60 cells, Salmonella were eliminated by hot water treatment at 65 or 70 C. Carcasses showed a partially cooked appearance after hot water treatment. Total bacterial counts on carcasses treated at 70 and 90 C, with or without added phosphate, and then chilled were lower than on control carcasses throughout storage at 2 C for up to 26 days. Phosphates did not consistently or significantly affect either Salmonella survival or total bacterial counts. Oxidative deterioration as measured by the thiobarbituric acid test was significantly retarded throughout 26 days of storage by the addition of 6% phosphate to a 70 or 90 C hot water treatment.", "contents": "Phosphate and heat treatments to control Salmonella and reduce spoilage and rancidity on broiler carcasses. Broiler chicken carcasses were inoculated with about 300 cells of a naladixic acid resistant strain of Salmonella typhimurium, then treated for 3 min with highly agitated water with or without 6% phosphate added, at 65, 70, 75, 80, 85, or 90 C. Other broiler carcasses were inoculated with about 60 cells, then similarly treated at 65 or 70 C. All carcasses were chilled after the hot water treatment in a system simulating commerical chilling. When inoculum was 300 cells, at least 5% of the carcasses remained contaminated with Salmonella, even when hot water treatment was at 90 C. When inoculum was 60 cells, Salmonella were eliminated by hot water treatment at 65 or 70 C. Carcasses showed a partially cooked appearance after hot water treatment. Total bacterial counts on carcasses treated at 70 and 90 C, with or without added phosphate, and then chilled were lower than on control carcasses throughout storage at 2 C for up to 26 days. Phosphates did not consistently or significantly affect either Salmonella survival or total bacterial counts. Oxidative deterioration as measured by the thiobarbituric acid test was significantly retarded throughout 26 days of storage by the addition of 6% phosphate to a 70 or 90 C hot water treatment."}
{"id": "PMID:471882", "title": "The effect of early treatment with methyl-CCNU on the lymphoid organs and the humoral immune response in chickens.", "content": "Presented are data upon the effect of a single or muliple administration of methyl-CCNU (a nitrosourea derivative an alkylating agent, and anticancer drug) to newly-hatched chicks. Lethal and sublethal doses of methyl-CCNU caused temporary damage to the lymphoid organs, of which the bursa was apparently the most sensitive. The decreased weight and the morphological changes observed in the bursa of treated chicks were accompanied by a parallel decrease in serum gamma-globulins level. The humoral immune response of animals treated with me-CCNU to sheep red blood cells and to killed Brucella abortus organisms was impaired in about 30% of the treated animals examined 8-10 weeks following 2 injections of 3 mg me-CCNU (given at the day of hatching and the next day). The thymus recovered rather rapidly from treatment. The time schedule of mortality and the early and late toxic manifestations of the drug as observed in chickens, in this study, are similar to previous reports obtained in mammals. Prolonged effects, however, were noted to some extent on body and spleen weight, and most dramatically, on testicular weight, morphology, and function.", "contents": "The effect of early treatment with methyl-CCNU on the lymphoid organs and the humoral immune response in chickens. Presented are data upon the effect of a single or muliple administration of methyl-CCNU (a nitrosourea derivative an alkylating agent, and anticancer drug) to newly-hatched chicks. Lethal and sublethal doses of methyl-CCNU caused temporary damage to the lymphoid organs, of which the bursa was apparently the most sensitive. The decreased weight and the morphological changes observed in the bursa of treated chicks were accompanied by a parallel decrease in serum gamma-globulins level. The humoral immune response of animals treated with me-CCNU to sheep red blood cells and to killed Brucella abortus organisms was impaired in about 30% of the treated animals examined 8-10 weeks following 2 injections of 3 mg me-CCNU (given at the day of hatching and the next day). The thymus recovered rather rapidly from treatment. The time schedule of mortality and the early and late toxic manifestations of the drug as observed in chickens, in this study, are similar to previous reports obtained in mammals. Prolonged effects, however, were noted to some extent on body and spleen weight, and most dramatically, on testicular weight, morphology, and function."}
{"id": "PMID:471883", "title": "Vaccination of chickens through the drinking water with a live, Pasteurella multocida, vaccine.", "content": "Broiler-type chickens were vaccinated orally at several ages with various dilutions of the Clemson University (CU) strain of Pasteurella multocida vaccine and then challenged orally with virulent strain P-1059 or strain X-73 of P. multocida to determine the degree of protection produced by vaccination. When vaccinated at 8 and 12 weeks of age for 2 consecutive days with a 1:133 dilution of the vaccine and then challenged at 17 and 19 weeks of age with strain X-73 and P-1059 P. multocida, respectively, no significant levels of protection were elicited in the vaccinates. Significant levels of protection were elicited in the vaccinates for 2 consecutive days at 9 weeks and at 13 weeks with 1:133 and 1:50 dilutions of the vaccine respectively, and then challenged at 16 weeks with strain X-73 and at 20 weeks with strain P-1059. Broilers vaccinated at 12 and 16 weeks for 3 consecutive days and then challenged at 20 weeks with strain X-73 showed a highly significant level of protection upon challenge when compared to the controls.", "contents": "Vaccination of chickens through the drinking water with a live, Pasteurella multocida, vaccine. Broiler-type chickens were vaccinated orally at several ages with various dilutions of the Clemson University (CU) strain of Pasteurella multocida vaccine and then challenged orally with virulent strain P-1059 or strain X-73 of P. multocida to determine the degree of protection produced by vaccination. When vaccinated at 8 and 12 weeks of age for 2 consecutive days with a 1:133 dilution of the vaccine and then challenged at 17 and 19 weeks of age with strain X-73 and P-1059 P. multocida, respectively, no significant levels of protection were elicited in the vaccinates. Significant levels of protection were elicited in the vaccinates for 2 consecutive days at 9 weeks and at 13 weeks with 1:133 and 1:50 dilutions of the vaccine respectively, and then challenged at 16 weeks with strain X-73 and at 20 weeks with strain P-1059. Broilers vaccinated at 12 and 16 weeks for 3 consecutive days and then challenged at 20 weeks with strain X-73 showed a highly significant level of protection upon challenge when compared to the controls."}
{"id": "PMID:471884", "title": "The effects of the northern fowl mite, Ornithonyssus sylivarium on egg production and body weight of caged White Leghorn hens.", "content": "Egg production of caged White Leghorn hens with heavy (greater than 1000) populations of the northen fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), was 5 to 15% less than egg production of control hens. Body weight was depressed in hens infested with mites before the hens came into full egg production but not when hens were infested after that time. Feed consumption was not affected by mite populations.", "contents": "The effects of the northern fowl mite, Ornithonyssus sylivarium on egg production and body weight of caged White Leghorn hens. Egg production of caged White Leghorn hens with heavy (greater than 1000) populations of the northen fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), was 5 to 15% less than egg production of control hens. Body weight was depressed in hens infested with mites before the hens came into full egg production but not when hens were infested after that time. Feed consumption was not affected by mite populations."}
{"id": "PMID:471885", "title": "Structure of the germinal centers in the chicken caecal tonsil: light and electron microscopic and autoradiographic studies.", "content": "The light and electron microscopic structure of the germinal center of chicken caecal tonsils were studied. Two kinds of germinal centers were revealed; the first was located deep in the lymphatic tissue close to the muscle layer of the caeca. These germinal centers frequently have an incomplete capsule, and are not, therefore, separated from the diffuse lymphatic tissue. The second kind of germinal center was located closer to the epithelium and had a complete capsule separating it from the diffuse lymphatic tissue. Both germinal centers possessed an outer dark cortical zone which contained smaller lymphoblasts (of less than 10 micron in diameter) and a central medullary area which contained larger lymphoblasts (up to 16 micron). Autoradiographic studies revealed that the small lymphoblasts of the outer cortical zone are concerned with cell generation and that the large lymphoblasts of the medullary area may have derived from the small lymphocytes. Within the medullary area of the germinal center are cells which are embedded in an intercellular substance of high density. We propose that the intercellular substance may be a product of this cell. For discussion purposes we have called this cell a secretory cell. The secretory cell may originate from a small lymphocyte-like cell.", "contents": "Structure of the germinal centers in the chicken caecal tonsil: light and electron microscopic and autoradiographic studies. The light and electron microscopic structure of the germinal center of chicken caecal tonsils were studied. Two kinds of germinal centers were revealed; the first was located deep in the lymphatic tissue close to the muscle layer of the caeca. These germinal centers frequently have an incomplete capsule, and are not, therefore, separated from the diffuse lymphatic tissue. The second kind of germinal center was located closer to the epithelium and had a complete capsule separating it from the diffuse lymphatic tissue. Both germinal centers possessed an outer dark cortical zone which contained smaller lymphoblasts (of less than 10 micron in diameter) and a central medullary area which contained larger lymphoblasts (up to 16 micron). Autoradiographic studies revealed that the small lymphoblasts of the outer cortical zone are concerned with cell generation and that the large lymphoblasts of the medullary area may have derived from the small lymphocytes. Within the medullary area of the germinal center are cells which are embedded in an intercellular substance of high density. We propose that the intercellular substance may be a product of this cell. For discussion purposes we have called this cell a secretory cell. The secretory cell may originate from a small lymphocyte-like cell."}
{"id": "PMID:471886", "title": "Plasma concentrations of progesterone and corticosterone during the ovulation cycle of the hen (Gallus domesticus).", "content": "The observation that injections of either ACTH or corticosterone induced ovulation in the hen has prompted speculation which implicates the adrenal gland in the mechanisms controlling ovulation. In this study, the role of the major adrenal steroid in birds was further examined by measuring the plasma concentration of corticosterone during the 24 hr period preceding mid-sequence ovipositions and the 28 hr period preceding terminal ovipositions. Progesterone, which is an accurate index of preovulatory ovarian activity, was also measured in the same samples. An increase in plasma progesterone preceded mid-sequence ovipositions which are accompanied by an ovulation. This preovulatory surge in plasma progesterone began approximately 7 hr before ovulation, reached a maximum of 6.9 ng/ml 3 to 2 hr before ovulation, and returned to baseline concentrations at the time of ovulation. A major preovulatory peak of progesterone was not observed during the interval between sequences, although a small but statistically significant rise in progesterone was observed between midnight and 0600 hr on the day of the last oviposition of the sequence. The concentration of corticosterone increased approximately two-fold during the dark portion of the photoperiod regardless of the position of the oviposition in the sequence. Baseline concentrations during the illuminated portion of the photoperiod ranged between 1 and 4 ng of corticosterone per ml of plasma whereas during the period of darkness, they ranged between 2 and 6 ng of corticosterone per ml of plasma. It was concluded, therefore, that the circulating concentration of corticosterone was regulated by a circadian rhythm which operates independently of follicular maturation.", "contents": "Plasma concentrations of progesterone and corticosterone during the ovulation cycle of the hen (Gallus domesticus). The observation that injections of either ACTH or corticosterone induced ovulation in the hen has prompted speculation which implicates the adrenal gland in the mechanisms controlling ovulation. In this study, the role of the major adrenal steroid in birds was further examined by measuring the plasma concentration of corticosterone during the 24 hr period preceding mid-sequence ovipositions and the 28 hr period preceding terminal ovipositions. Progesterone, which is an accurate index of preovulatory ovarian activity, was also measured in the same samples. An increase in plasma progesterone preceded mid-sequence ovipositions which are accompanied by an ovulation. This preovulatory surge in plasma progesterone began approximately 7 hr before ovulation, reached a maximum of 6.9 ng/ml 3 to 2 hr before ovulation, and returned to baseline concentrations at the time of ovulation. A major preovulatory peak of progesterone was not observed during the interval between sequences, although a small but statistically significant rise in progesterone was observed between midnight and 0600 hr on the day of the last oviposition of the sequence. The concentration of corticosterone increased approximately two-fold during the dark portion of the photoperiod regardless of the position of the oviposition in the sequence. Baseline concentrations during the illuminated portion of the photoperiod ranged between 1 and 4 ng of corticosterone per ml of plasma whereas during the period of darkness, they ranged between 2 and 6 ng of corticosterone per ml of plasma. It was concluded, therefore, that the circulating concentration of corticosterone was regulated by a circadian rhythm which operates independently of follicular maturation."}
{"id": "PMID:471887", "title": "The effect of photoperiod and feed restriction on semen production in the turkey.", "content": "In Experiment 1, 72 yearling Broad Breasted White Turkey males were induced to molt under either stimulatory (16L:8D) or nonstimulatory (8L:16D) light used concurrently with a short period of feed and water deprivation, and followed by periods of daily feed restriction (50% of full feed). One-fourth of the males wer killed at intervals of 4, 8, 12, and 16 weeks and their testes weighed. In Experiment 2, 40 yearling Broad Breasted White males were killed at 2-week intervals during an 8-week period of 8 hr light per day followed by a 10-week period of 16 hr light per day. Testes were removed, weighed, and fixed in Bouin's solution for histological preparation. In Experiment 1, birds kept under 16 hr light and given different periods of feed and water deprivation, followed by 8 weeks of feed restriction, failed to terminate semen production, although their testes were reduced substantially in size and function. Other groups of males given 8 hr light per day with concurrent regimens of feed deprivation and daily feed restriction, terminated semen production within 4 weeks. When returned to 16 hr light, the testes were twice as heavy as those of males kept in production during the entire test. In Experiment 2, the testes of birds induced to molt under 8 hr light per day regressed by 73% and 89%, respectively, after 4 and 8 weeks of exposure. When given 16 hr light per day, some spermatogenic activity was noted at 4 weeks, and complete spermatogenesis, with maximum semen production, occurred after 6 weeks. There was evidence that a successful rejuvenation of the testes requires a period of complete quiescence.", "contents": "The effect of photoperiod and feed restriction on semen production in the turkey. In Experiment 1, 72 yearling Broad Breasted White Turkey males were induced to molt under either stimulatory (16L:8D) or nonstimulatory (8L:16D) light used concurrently with a short period of feed and water deprivation, and followed by periods of daily feed restriction (50% of full feed). One-fourth of the males wer killed at intervals of 4, 8, 12, and 16 weeks and their testes weighed. In Experiment 2, 40 yearling Broad Breasted White males were killed at 2-week intervals during an 8-week period of 8 hr light per day followed by a 10-week period of 16 hr light per day. Testes were removed, weighed, and fixed in Bouin's solution for histological preparation. In Experiment 1, birds kept under 16 hr light and given different periods of feed and water deprivation, followed by 8 weeks of feed restriction, failed to terminate semen production, although their testes were reduced substantially in size and function. Other groups of males given 8 hr light per day with concurrent regimens of feed deprivation and daily feed restriction, terminated semen production within 4 weeks. When returned to 16 hr light, the testes were twice as heavy as those of males kept in production during the entire test. In Experiment 2, the testes of birds induced to molt under 8 hr light per day regressed by 73% and 89%, respectively, after 4 and 8 weeks of exposure. When given 16 hr light per day, some spermatogenic activity was noted at 4 weeks, and complete spermatogenesis, with maximum semen production, occurred after 6 weeks. There was evidence that a successful rejuvenation of the testes requires a period of complete quiescence."}
{"id": "PMID:471888", "title": "Description and surgical removal of the cranial cervical ganglia in 3- to 5-day-old chicks.", "content": "The cranial cervical sympathetic ganglion (CCG) provides the primary innervation of the pineal gland in several gallinaceous species. The CCG is located at the base of the skull near the exoccipital bone, dorsal to the level of the vagus and glossopharyngeal nerves. It occupies a much larger volume and appears pinkish-gray, instead of white, when compared to the petrosal ganglion. To surgically remove the CCG, chicks were anesthetized with halothane vapor. Following a small skin incision, blunt dissection was used to expose the CCG lying adjacent to the internal carotid. The ganglion was grasped with small forceps and pinched free of its fine neural connections to adjacent nerves. The success of the surgery was confirmed visually and by complete adrenergic dennervation of the pineal gland. The entire surgical procedure required approximately 20 min per bird. Mortality was less than 20% overall.", "contents": "Description and surgical removal of the cranial cervical ganglia in 3- to 5-day-old chicks. The cranial cervical sympathetic ganglion (CCG) provides the primary innervation of the pineal gland in several gallinaceous species. The CCG is located at the base of the skull near the exoccipital bone, dorsal to the level of the vagus and glossopharyngeal nerves. It occupies a much larger volume and appears pinkish-gray, instead of white, when compared to the petrosal ganglion. To surgically remove the CCG, chicks were anesthetized with halothane vapor. Following a small skin incision, blunt dissection was used to expose the CCG lying adjacent to the internal carotid. The ganglion was grasped with small forceps and pinched free of its fine neural connections to adjacent nerves. The success of the surgery was confirmed visually and by complete adrenergic dennervation of the pineal gland. The entire surgical procedure required approximately 20 min per bird. Mortality was less than 20% overall."}
{"id": "PMID:471889", "title": "Morphological and histochemical observations on the primordial and early growing oocytes of crow (Corvus splendens) and myna (Acridotheres tristis).", "content": "A morphological and histochemical study has been made of the primordial and early growing oocytes in the ovaries of crow (Corvus splendens) and common myna (Acridotheres tristis). The primordial oocytes in the myna ovary are loosely arranged in groups or nests, whereas in crow they form compact nests surrounded by highly vascularized connective tissue bands or lie in layers beneath the surface epithelium. The primordial oocytes in both the species are surrounded by flat granulosa cells whose number, shape, and cytochemical properties change with the initiation of growth. The oocyte nucleus shows a single basophilic nucleolus and thick diplotene chromosomes. With the initiation of growth, the number of nucleoli increases; simultaneously the chromosomes attain lampbrush configuration. Crescent-shaped Balbiani's vitelline body consists of ribonucleoproteins, lipoproteins, and phospholipids. The amount of these substances increases with the oocyte growth. The nature of proteins and lipids in the ooplasm and follicular epithelium also changes with the oocyte growth. Some randomly distributed protein bodies are also present in the ooplasm of primordial follicles. They disappear with the initiation of oocyte growth. The enzyme activities of acid phosphatase, NADP-diaphorase and NAD-diaphorase, also increase in the Balbiani's vitelline body with the oocyte growth. Alkaline phosphatase and delta 5-3 beta-HSDH activities are not seen. The possible functional significance of these morphological and histochemical changes has been discussed in relation to the initiation of growth in quiescent oocytes.", "contents": "Morphological and histochemical observations on the primordial and early growing oocytes of crow (Corvus splendens) and myna (Acridotheres tristis). A morphological and histochemical study has been made of the primordial and early growing oocytes in the ovaries of crow (Corvus splendens) and common myna (Acridotheres tristis). The primordial oocytes in the myna ovary are loosely arranged in groups or nests, whereas in crow they form compact nests surrounded by highly vascularized connective tissue bands or lie in layers beneath the surface epithelium. The primordial oocytes in both the species are surrounded by flat granulosa cells whose number, shape, and cytochemical properties change with the initiation of growth. The oocyte nucleus shows a single basophilic nucleolus and thick diplotene chromosomes. With the initiation of growth, the number of nucleoli increases; simultaneously the chromosomes attain lampbrush configuration. Crescent-shaped Balbiani's vitelline body consists of ribonucleoproteins, lipoproteins, and phospholipids. The amount of these substances increases with the oocyte growth. The nature of proteins and lipids in the ooplasm and follicular epithelium also changes with the oocyte growth. Some randomly distributed protein bodies are also present in the ooplasm of primordial follicles. They disappear with the initiation of oocyte growth. The enzyme activities of acid phosphatase, NADP-diaphorase and NAD-diaphorase, also increase in the Balbiani's vitelline body with the oocyte growth. Alkaline phosphatase and delta 5-3 beta-HSDH activities are not seen. The possible functional significance of these morphological and histochemical changes has been discussed in relation to the initiation of growth in quiescent oocytes."}
{"id": "PMID:471890", "title": "Arprinocoid evaluation in broiler chicken pen trials in Europe.", "content": "The effect of arpinocid (9-[2-chloro-6-fluorophenyl)methyl]-9H-purin-6-amine) on artificial and natural coccidial infections in broiler chickens was evaluated in a series of pen trials conducted in England, France, and Germany. In trials with induced infections, mortality attributable to coccidiosis did not occur at 50, 60, and 70 ppm although it ranged from 3.2 to 9% in unmedicated groups. At 7 to 8 weeks of age, birds receiving arprinocoid in feed at levels of 40, 50, 60, and 70 ppm had improved body weights and feed conversion compared with unmedicated birds, the differences being significant (P is less than .05) in 4 out of 5 trials. Productivity of birds on arprinocid was equivalent or significantly superior (P is less than 0.5) to those on halofuginone (3 ppm), monensin (100 ppm), nicarbazin (125 ppm), and PANCOXIN (165 ppm). Based on the results of these pen trials, arprinocid at levels of 50 to 70 ppm in feed is a highly efficacious compound for the prophylaxis of coccidiosis, preventing mortality, and permitting good growth and feed conversion under conditions of both mild and severe infection pressure.", "contents": "Arprinocoid evaluation in broiler chicken pen trials in Europe. The effect of arpinocid (9-[2-chloro-6-fluorophenyl)methyl]-9H-purin-6-amine) on artificial and natural coccidial infections in broiler chickens was evaluated in a series of pen trials conducted in England, France, and Germany. In trials with induced infections, mortality attributable to coccidiosis did not occur at 50, 60, and 70 ppm although it ranged from 3.2 to 9% in unmedicated groups. At 7 to 8 weeks of age, birds receiving arprinocoid in feed at levels of 40, 50, 60, and 70 ppm had improved body weights and feed conversion compared with unmedicated birds, the differences being significant (P is less than .05) in 4 out of 5 trials. Productivity of birds on arprinocid was equivalent or significantly superior (P is less than 0.5) to those on halofuginone (3 ppm), monensin (100 ppm), nicarbazin (125 ppm), and PANCOXIN (165 ppm). Based on the results of these pen trials, arprinocid at levels of 50 to 70 ppm in feed is a highly efficacious compound for the prophylaxis of coccidiosis, preventing mortality, and permitting good growth and feed conversion under conditions of both mild and severe infection pressure."}
{"id": "PMID:471891", "title": "Ultrastructure of the turkey's basilar papilla and the damping elements in the scala media.", "content": "The ultrastructure of the turkey's inner ear was investigated by use of scanning and transmission electron microscopes. It was shown that hair cells of the basilar papilla are separated from each other by microvilli of the supporting cells. Organized compartments in the lower surface of the tectorial membrane of the turkey are arranged in polarized fashion as are the hair bundles. Kinocilia were not identified in the hair bundles examined in this study. Well-organized filamentous elements were observed in the cutucular plates. The continuation of these filamentous elements into the stereocilia indicates that the theory of adjustable resistor in generating cochlear potential may be closely associated with these elements. Membrane bound homogeneous material was found in the scala media between the tegmentum vasculosum and the tectorial membrane. The presence of this homogeneous damping material is believed to be essential in offsetting the sensitivity and enhancing the time resolution ability of the relatively short avian cochlea.", "contents": "Ultrastructure of the turkey's basilar papilla and the damping elements in the scala media. The ultrastructure of the turkey's inner ear was investigated by use of scanning and transmission electron microscopes. It was shown that hair cells of the basilar papilla are separated from each other by microvilli of the supporting cells. Organized compartments in the lower surface of the tectorial membrane of the turkey are arranged in polarized fashion as are the hair bundles. Kinocilia were not identified in the hair bundles examined in this study. Well-organized filamentous elements were observed in the cutucular plates. The continuation of these filamentous elements into the stereocilia indicates that the theory of adjustable resistor in generating cochlear potential may be closely associated with these elements. Membrane bound homogeneous material was found in the scala media between the tegmentum vasculosum and the tectorial membrane. The presence of this homogeneous damping material is believed to be essential in offsetting the sensitivity and enhancing the time resolution ability of the relatively short avian cochlea."}
{"id": "PMID:471892", "title": "Influence of exogenous avian pancreatic polypeptide on gastrointestinal motility in turkeys.", "content": "To determine the influence of avian pancreatic polypeptide (APP) on avian GI motility, strain-gauge transducers were implanted on the glandular stomach, thick caudodorsal and thin caudoventral muscles of the muscular stomach, and on the duodenum (cranial tract) of five young turkeys. Implants were also made on the ileum, cecum, and colon (caudal tract) of three other turkeys. Isovolumic injections of APP at six (cranial tract preparations) or four (caudal tract preparations) levels were made via a chronic jugular catheter while recording GI contractile activity in fasted birds. Injections of 2 or 5 micrograms/kg caused no statistically significant change in motility of the cranial tract. Significant depression in contraction frequency during the first 10 min post-injection resulted from an injection of 8 micrograms/kg. Injections of 10, 20, and 30 micrograms/kg depressed motility throughout the entire 30 min post-injection period. Motility of the caudal tract usually was not significantly affected by injections of 5 and 10 micrograms/kg doses. Larger doses (20 and 30 micrograms/kg) significantly depressed caudal tract motility during the first 10 min post-injection but not throughout the 30 min post-injection period. In both cranial and caudal portions of the tract, depression of contractile activity by injections of APP persisted longer following larger doses. The highest plasma APP levels in turkeys, found at about 1 hr post-prandially, were still less than plasma levels following IV injection of 5 micrograms/kg. Since the latter injection caused no apparent alteration in Gi motility, APP may have little or no physiological role in regulation of avian GI motility.", "contents": "Influence of exogenous avian pancreatic polypeptide on gastrointestinal motility in turkeys. To determine the influence of avian pancreatic polypeptide (APP) on avian GI motility, strain-gauge transducers were implanted on the glandular stomach, thick caudodorsal and thin caudoventral muscles of the muscular stomach, and on the duodenum (cranial tract) of five young turkeys. Implants were also made on the ileum, cecum, and colon (caudal tract) of three other turkeys. Isovolumic injections of APP at six (cranial tract preparations) or four (caudal tract preparations) levels were made via a chronic jugular catheter while recording GI contractile activity in fasted birds. Injections of 2 or 5 micrograms/kg caused no statistically significant change in motility of the cranial tract. Significant depression in contraction frequency during the first 10 min post-injection resulted from an injection of 8 micrograms/kg. Injections of 10, 20, and 30 micrograms/kg depressed motility throughout the entire 30 min post-injection period. Motility of the caudal tract usually was not significantly affected by injections of 5 and 10 micrograms/kg doses. Larger doses (20 and 30 micrograms/kg) significantly depressed caudal tract motility during the first 10 min post-injection but not throughout the 30 min post-injection period. In both cranial and caudal portions of the tract, depression of contractile activity by injections of APP persisted longer following larger doses. The highest plasma APP levels in turkeys, found at about 1 hr post-prandially, were still less than plasma levels following IV injection of 5 micrograms/kg. Since the latter injection caused no apparent alteration in Gi motility, APP may have little or no physiological role in regulation of avian GI motility."}
{"id": "PMID:471893", "title": "Plasma corticosterone values in genetic hypertensive and hypotensive strains of turkeys.", "content": "Plasma corticosterone levels of hypertensive and hypostensive turkeys were 2.50 and 2.19 micrograms/dl, respectively. Male turkeys of both strains had a mean plasma levels of 2.47 micrograms/dl and female turkeys had a mean level of 2.21 micrograms/gl. The average plasma corticosterone level for all turkeys was 2.35 micrograms/dl. Although the plasma corticosterone levels were higher at 1200 hr than at either 0800 or 1600 hr, the differences were not statistically significant. It was condluded that blood pressure selection had no significant effect on plasma corticosterone levels.", "contents": "Plasma corticosterone values in genetic hypertensive and hypotensive strains of turkeys. Plasma corticosterone levels of hypertensive and hypostensive turkeys were 2.50 and 2.19 micrograms/dl, respectively. Male turkeys of both strains had a mean plasma levels of 2.47 micrograms/dl and female turkeys had a mean level of 2.21 micrograms/gl. The average plasma corticosterone level for all turkeys was 2.35 micrograms/dl. Although the plasma corticosterone levels were higher at 1200 hr than at either 0800 or 1600 hr, the differences were not statistically significant. It was condluded that blood pressure selection had no significant effect on plasma corticosterone levels."}
{"id": "PMID:471894", "title": "Response of hens to various dietary levels of tannic acid.", "content": "One-hundred-twenty Babcock B-300 ehns, 17 months of age, were placed one bird per cage with six replicates of five birds each per treatment. Four levels (0, 1, 2, and 4%) of technical grade tannic acid were added to a standard corn-soy layer diet. With the exception of feed comsumption during the second and third week, there were no significant differences for feed consumption, egg production, feed efficiency, fertility, or hatchability amond the control birds and those receiving tannic acid at the 1% level. The addition of tannic acid at levels of 2 and 4% resulted in significantly decreased feed consumption and egg production during the six-week experimental period. Fertility and hatchability were not significantly affected by dietary tannic acid.", "contents": "Response of hens to various dietary levels of tannic acid. One-hundred-twenty Babcock B-300 ehns, 17 months of age, were placed one bird per cage with six replicates of five birds each per treatment. Four levels (0, 1, 2, and 4%) of technical grade tannic acid were added to a standard corn-soy layer diet. With the exception of feed comsumption during the second and third week, there were no significant differences for feed consumption, egg production, feed efficiency, fertility, or hatchability amond the control birds and those receiving tannic acid at the 1% level. The addition of tannic acid at levels of 2 and 4% resulted in significantly decreased feed consumption and egg production during the six-week experimental period. Fertility and hatchability were not significantly affected by dietary tannic acid."}
{"id": "PMID:471895", "title": "Practical immunization of chickens against coccidiosis using an attenuated strain of Eimeria tenella.", "content": "Three floor-pen trials were conducted to compare two attenuated strains of Eimeria tenella (Wis-F-96 and Wis-F-125) with the strain of E. tenella used in a commercial planned immunization program (Coccivac). Broiler cockerels were started in floor pens on new pine shavings litter. Immunizing oocysts were administered in the drinking water during the first week with total dose per bird of 175 oocysts of the control strain (Coccivac); 175, 350, or 700 oocysts of the Wis-F-125; 350 oocysts of Wis-F-96; or no oocysts (non-seeded control). The control strain was more pathogenic than the Wis-F-125 or the Wis-F-96 strains as indicated by significantly higher lesion scores 17 days after initial exposure and by loss of two birds in the control strain exposed groups. Low lesion scores and no deaths due to coccidiosis characterized the use of the Wis-F-125 strain. Flock immunity, as determined by immunity challenge, was equivalent between the control strain and the higher dosages of Wis-F-125. The Wis-F-96 strain did not adequately immunize chickens in these experiments.", "contents": "Practical immunization of chickens against coccidiosis using an attenuated strain of Eimeria tenella. Three floor-pen trials were conducted to compare two attenuated strains of Eimeria tenella (Wis-F-96 and Wis-F-125) with the strain of E. tenella used in a commercial planned immunization program (Coccivac). Broiler cockerels were started in floor pens on new pine shavings litter. Immunizing oocysts were administered in the drinking water during the first week with total dose per bird of 175 oocysts of the control strain (Coccivac); 175, 350, or 700 oocysts of the Wis-F-125; 350 oocysts of Wis-F-96; or no oocysts (non-seeded control). The control strain was more pathogenic than the Wis-F-125 or the Wis-F-96 strains as indicated by significantly higher lesion scores 17 days after initial exposure and by loss of two birds in the control strain exposed groups. Low lesion scores and no deaths due to coccidiosis characterized the use of the Wis-F-125 strain. Flock immunity, as determined by immunity challenge, was equivalent between the control strain and the higher dosages of Wis-F-125. The Wis-F-96 strain did not adequately immunize chickens in these experiments."}
{"id": "PMID:471896", "title": "Effects of feeding tylosin to layers exposed to Mycoplasma gallisepitcum.", "content": "Two experiments are reported in which the effect of tylosin upon the development of Mycoplasma gallisepticum (MG) infection in replacement layers was monitored by serological and cultural methods. Concentrations of tylosin tested included 0, 22, 38.5, and 55 ppm. Egg production and feed consumption data were collected for one year. Tylosin slowed the rate of spread of MG infection as measured by serological and cultural methods. The effect of tylosin on the spread of MG infection was dose related. Hen day egg production peaked at 85 to 87% in the mediated groups, while controls peaked at 81 to 84%. The magnitude of tylosin effect on production increased with time to a maximum difference of 10 to 12% at 52 weeks. Feed efficiency was markedly improved by all concentration of tylosin ranging from 5% at peak production to 12 to 18% at 52 weeks. The incidence of shell-less or soft shelled eggs was markedly reduced in the tylosin treated group.", "contents": "Effects of feeding tylosin to layers exposed to Mycoplasma gallisepitcum. Two experiments are reported in which the effect of tylosin upon the development of Mycoplasma gallisepticum (MG) infection in replacement layers was monitored by serological and cultural methods. Concentrations of tylosin tested included 0, 22, 38.5, and 55 ppm. Egg production and feed consumption data were collected for one year. Tylosin slowed the rate of spread of MG infection as measured by serological and cultural methods. The effect of tylosin on the spread of MG infection was dose related. Hen day egg production peaked at 85 to 87% in the mediated groups, while controls peaked at 81 to 84%. The magnitude of tylosin effect on production increased with time to a maximum difference of 10 to 12% at 52 weeks. Feed efficiency was markedly improved by all concentration of tylosin ranging from 5% at peak production to 12 to 18% at 52 weeks. The incidence of shell-less or soft shelled eggs was markedly reduced in the tylosin treated group."}
{"id": "PMID:471897", "title": "A darkness induced eye abnormality in the domestic chicken.", "content": "Two-hundred broiler-type chickens were reared under either normal lighting or darkness for 16 weeks. Each week, birds were subjected to applanation tonometry for measurement of intraocular pressures (IOP). The birds were then weighed, eye enucleated, and dimensions measured. All eyes were prepared for histological examination. Chickens reared in darkness for 4 to 6 weeks displayed an abnormal eye enlargement, accompanied by an increase in IOP in comparison to chickens reared under conventional management. From the 6th to the 16th week, the eyes of dark-reared chickens continued to enlarge while IOP decreased. The sclera, choroid, retina, and retinal layers of dark-reared chicks were not as thick as controls throughout the 16 weeks. Two principle retinal pathologies were induced by darkness. One appeared as darkened areas of the peripheral retina. In histological sections the darkened retinal areas had a thin choroid and thin retinal layers. The second retinal abnormality was one or more white, nonpigmented bands directed perpendicular to the pectin. Histologically, the depigmented bands were areas in which the vessels of the choroid, the pigmented epithelium, and the outer retinal layers were absent. The corneas of dark-reared chicks exhibited a reduction in their curvature and thickness compared to control corneas.", "contents": "A darkness induced eye abnormality in the domestic chicken. Two-hundred broiler-type chickens were reared under either normal lighting or darkness for 16 weeks. Each week, birds were subjected to applanation tonometry for measurement of intraocular pressures (IOP). The birds were then weighed, eye enucleated, and dimensions measured. All eyes were prepared for histological examination. Chickens reared in darkness for 4 to 6 weeks displayed an abnormal eye enlargement, accompanied by an increase in IOP in comparison to chickens reared under conventional management. From the 6th to the 16th week, the eyes of dark-reared chickens continued to enlarge while IOP decreased. The sclera, choroid, retina, and retinal layers of dark-reared chicks were not as thick as controls throughout the 16 weeks. Two principle retinal pathologies were induced by darkness. One appeared as darkened areas of the peripheral retina. In histological sections the darkened retinal areas had a thin choroid and thin retinal layers. The second retinal abnormality was one or more white, nonpigmented bands directed perpendicular to the pectin. Histologically, the depigmented bands were areas in which the vessels of the choroid, the pigmented epithelium, and the outer retinal layers were absent. The corneas of dark-reared chicks exhibited a reduction in their curvature and thickness compared to control corneas."}
{"id": "PMID:471898", "title": "A comparison of methods for exposing chickens to Coccidiosis in floor-pen trials.", "content": "Three floor-pen trials were conducted for the purpose of evaluating 4 methods of exposing broiler chickens to coccidiosis. The methods were compared on the basis of their effect on weight, feed efficiency, histological lesions, and mortality. In each of the individual trials, birds exposed to coccidiosis via the feed had significantly (P is less than 0.5) higher incidence of light to severe histological lesions that those exposed via \"seeder birds\" or contaminated litter. On the basis of pooled data from the three trials, birds exposed via the feed had lower feed efficiency, higher mortality, and significantly (P is less than 0.5) lower weight at 8 weeks than those exposed via the other techniques. However, the feed method of exposure resulted in a significantly higher variability (P is less than 0.1) in mortality among pens. For uniformity of infection, it was concluded that the most satisfactory method of exposing birds to coccidiosis in floor pen trials was to spread laboratory sportulated oocysts over the litter.", "contents": "A comparison of methods for exposing chickens to Coccidiosis in floor-pen trials. Three floor-pen trials were conducted for the purpose of evaluating 4 methods of exposing broiler chickens to coccidiosis. The methods were compared on the basis of their effect on weight, feed efficiency, histological lesions, and mortality. In each of the individual trials, birds exposed to coccidiosis via the feed had significantly (P is less than 0.5) higher incidence of light to severe histological lesions that those exposed via \"seeder birds\" or contaminated litter. On the basis of pooled data from the three trials, birds exposed via the feed had lower feed efficiency, higher mortality, and significantly (P is less than 0.5) lower weight at 8 weeks than those exposed via the other techniques. However, the feed method of exposure resulted in a significantly higher variability (P is less than 0.1) in mortality among pens. For uniformity of infection, it was concluded that the most satisfactory method of exposing birds to coccidiosis in floor pen trials was to spread laboratory sportulated oocysts over the litter."}
{"id": "PMID:471899", "title": "Effect of sexual experience, location, malnutrition, and repeated sampling on concentrations of testosterone in blood plasma of Gallus domesticus roosters.", "content": "Five studies were conducted with mature White Leghorn roosters to study variables associated with concentrations of testosterone in blood sampled from a wing vein. Testosterone in blood plasma was unchanged 15, 30, or 45 min after collection of semen artificially whether or not roosters were trained for collection of semen. Likewise, cohabitation with hens for 7 to 8 hr was without effect. However, trained roosters had higher (P less than .05) plasma testosterone concentrations after 7 to 8 hr in a new location than untrained roosters. Furthermore, both groups in the new location had higher testosterone concentrations than the controls not translocated. Plasma testosterone was decreased (P less than .01) about 50% within 2 weeks by feeding a diet low in crude protein (2%) which decreased feed intake 33% and caused weight loss; those fed 12% crude proteins were unchanged. Variations in concentrations of testosterone among sequential samples of blood collected at 45 min intervals for 3 hr indicated random pulsatile releases of testosterone as reported for mammals. Sequential sampling at 15 min intervals for 1 to 2 hr revealed that the testosterone increases (up to 10 ng/ml) lasted for about 1 hr and that the returns to basal concentrations required about 1 to 1.5 hr. The interval between pulsatile releases may be as long as 3 hr in some roosters. These results indicate that roosters have spontaneous and pulsatile releases of testosterone as expected, because roosters release luteinizing hormone episodically during both light and dark cycles of the day.", "contents": "Effect of sexual experience, location, malnutrition, and repeated sampling on concentrations of testosterone in blood plasma of Gallus domesticus roosters. Five studies were conducted with mature White Leghorn roosters to study variables associated with concentrations of testosterone in blood sampled from a wing vein. Testosterone in blood plasma was unchanged 15, 30, or 45 min after collection of semen artificially whether or not roosters were trained for collection of semen. Likewise, cohabitation with hens for 7 to 8 hr was without effect. However, trained roosters had higher (P less than .05) plasma testosterone concentrations after 7 to 8 hr in a new location than untrained roosters. Furthermore, both groups in the new location had higher testosterone concentrations than the controls not translocated. Plasma testosterone was decreased (P less than .01) about 50% within 2 weeks by feeding a diet low in crude protein (2%) which decreased feed intake 33% and caused weight loss; those fed 12% crude proteins were unchanged. Variations in concentrations of testosterone among sequential samples of blood collected at 45 min intervals for 3 hr indicated random pulsatile releases of testosterone as reported for mammals. Sequential sampling at 15 min intervals for 1 to 2 hr revealed that the testosterone increases (up to 10 ng/ml) lasted for about 1 hr and that the returns to basal concentrations required about 1 to 1.5 hr. The interval between pulsatile releases may be as long as 3 hr in some roosters. These results indicate that roosters have spontaneous and pulsatile releases of testosterone as expected, because roosters release luteinizing hormone episodically during both light and dark cycles of the day."}
{"id": "PMID:471900", "title": "Floor pen studies on the anticoccidial efficacy of arprinocid in turkeys.", "content": "Arprinocid was tested against coccidiosis in turkeys reared in floor pens. Contamination of pens with Eimeria meleagrimitis, E. adenoeides, and E. gallopavonis was accomplished by the infected seeder bird technique. Performance improved with increasing levels of arprinocid through 120 ppm, but weight gains were depressed at 180 ppm. Lesion scores were significantly and progressively reduced by treatment with 60, 90, 120, and 180 ppm of arprinocid. Control of lesion scores and performance of birds treated with 120 ppm of arprinocid were comparable to the amprolium (125 ppm) treatment. In a separate experiment, the oocyst passage of E. meleagrimitis and of E. adenoeides was progressively reduced by increasing levels of arprinocid, and sporulation rate was decreased. Sportulation was not obtained in oocysts from birds fed arprinocid at 120 ppm or higher.", "contents": "Floor pen studies on the anticoccidial efficacy of arprinocid in turkeys. Arprinocid was tested against coccidiosis in turkeys reared in floor pens. Contamination of pens with Eimeria meleagrimitis, E. adenoeides, and E. gallopavonis was accomplished by the infected seeder bird technique. Performance improved with increasing levels of arprinocid through 120 ppm, but weight gains were depressed at 180 ppm. Lesion scores were significantly and progressively reduced by treatment with 60, 90, 120, and 180 ppm of arprinocid. Control of lesion scores and performance of birds treated with 120 ppm of arprinocid were comparable to the amprolium (125 ppm) treatment. In a separate experiment, the oocyst passage of E. meleagrimitis and of E. adenoeides was progressively reduced by increasing levels of arprinocid, and sporulation rate was decreased. Sportulation was not obtained in oocysts from birds fed arprinocid at 120 ppm or higher."}
{"id": "PMID:471901", "title": "Residues and effects from feeding high concentrations of hexachlorobenzene to broiler cockerels.", "content": "Eight-day old broiler cockerels received 0, 1, 10, or 100 ppm dietary hexachlorobenzene (HCB) for 25, 38, or 52 days. Treated birds grew faster for the first 25 days, but the trend was reversed at later times so there was no net weight difference at 52 days. Birds receiving 100 ppm dietary HCB had significantly enlarged adrenal glands at 38 days and enlarged livers at 38 and 52 days. Equilibrium concentrations in adipose tissue 12 to 13 times the dietary concentration were reached by 23 days in birds receiving 10 or 100 ppm HCB. Birds receiving 1 ppm concentrated HCB more effectively in the fat and did not reach equilibrium until 23 to 38 days.", "contents": "Residues and effects from feeding high concentrations of hexachlorobenzene to broiler cockerels. Eight-day old broiler cockerels received 0, 1, 10, or 100 ppm dietary hexachlorobenzene (HCB) for 25, 38, or 52 days. Treated birds grew faster for the first 25 days, but the trend was reversed at later times so there was no net weight difference at 52 days. Birds receiving 100 ppm dietary HCB had significantly enlarged adrenal glands at 38 days and enlarged livers at 38 and 52 days. Equilibrium concentrations in adipose tissue 12 to 13 times the dietary concentration were reached by 23 days in birds receiving 10 or 100 ppm HCB. Birds receiving 1 ppm concentrated HCB more effectively in the fat and did not reach equilibrium until 23 to 38 days."}
{"id": "PMID:471902", "title": "Effects of different short-term dietary phosphorus levels on egg specific gravity and blood phosphorus of hens.", "content": "In the first 2 experiments, laying hens were fed a diet containing .30% phosphorus for a preliminary period of 3 days. At the beginning of the experimental period, hens were fed .30, .75, or 1.40% phosphorus with 3.50% calcium. Another group of hens was fed .75% phosphorus and 1.40% calcium. Specific gravity of the eggs and blood serum inorganic phosphorus were determined and compared with those from control hens continuously fed a diet containing .75% phosphorus and 3.50% calcium. Both high phosphorus and/or low calcium decreased the specific gravity of eggs and increased the serum phosphorus level. In experiment 2, hens were fed different levels of phosphorus at different times of the day. Birds receiving 1.40% phosphorus between 0700 and 0930 hr and .30% phosphorus between 0930 and 2000 hr (Treatment 2) laid eggs with higher specific gravity than hens fed .30% phosphorus between 0700 and 0930 hr and 1.40% phosphorus between 0930 and 2000 hr (Treatment 3). there was no further improvement in egg shell quality over that from hens fed .75% phosphorus for both time periods (0700 to 2000 hr). In both experiments it was apparent that the dietary phosphorus level affected egg shell quality.", "contents": "Effects of different short-term dietary phosphorus levels on egg specific gravity and blood phosphorus of hens. In the first 2 experiments, laying hens were fed a diet containing .30% phosphorus for a preliminary period of 3 days. At the beginning of the experimental period, hens were fed .30, .75, or 1.40% phosphorus with 3.50% calcium. Another group of hens was fed .75% phosphorus and 1.40% calcium. Specific gravity of the eggs and blood serum inorganic phosphorus were determined and compared with those from control hens continuously fed a diet containing .75% phosphorus and 3.50% calcium. Both high phosphorus and/or low calcium decreased the specific gravity of eggs and increased the serum phosphorus level. In experiment 2, hens were fed different levels of phosphorus at different times of the day. Birds receiving 1.40% phosphorus between 0700 and 0930 hr and .30% phosphorus between 0930 and 2000 hr (Treatment 2) laid eggs with higher specific gravity than hens fed .30% phosphorus between 0700 and 0930 hr and 1.40% phosphorus between 0930 and 2000 hr (Treatment 3). there was no further improvement in egg shell quality over that from hens fed .75% phosphorus for both time periods (0700 to 2000 hr). In both experiments it was apparent that the dietary phosphorus level affected egg shell quality."}
{"id": "PMID:471939", "title": "A rapid purification procedure for L-asparaginase from Vibrio succinogenes.", "content": "A simple procedure has been developed for the purification of L-asparaginase from Vibrio succinogenes. Only two steps of ion-exchange chromatography are required. A higher yield and higher specific activity are obtained than previously reported.", "contents": "A rapid purification procedure for L-asparaginase from Vibrio succinogenes. A simple procedure has been developed for the purification of L-asparaginase from Vibrio succinogenes. Only two steps of ion-exchange chromatography are required. A higher yield and higher specific activity are obtained than previously reported."}
{"id": "PMID:471940", "title": "Isolation and partial characterization of kinin-like peptides formed by acid protease from murine fibroblast L-929.", "content": "A kinin-forming acid protease was isolated and purified from a stationary cell culture of murine fibroblasts L-929 (Biochem. Pharmacol. 26: 1187, 1977). The enzyme formed kinins at an optimal pH of 4.0 from Murphy-Sturm lymphosarcoma tissue and rat plasma kininogen substrate. This study reports on the isolation and partial chemical characterization of two fibroblast kinins. The 12,000 g fraction of L-929 fibroblast homogenates was incubated with rat plasma at 37 degrees, pH 4.0, for 92 hours, and the kinins extracted with ethyl alcohol-p-toluene sulfonic acid. Two fibroblast kinins, FKI and FKII, were separated and purified on the following chromatographic columns: G-25 Sephadex (1 X 115 cm), CM-C50 Sephadex (2.5 X 15 cm), and Biogel P-4 (1 X 115 cm). Kinin activity was bioassayed on the perfused isolated rat uterus preparation. The purity of the kinins was ascertained by thin layer chromatography coupled with dansylation. The estimated molecular weight of FKI was 1450 with a 14-amino acid composition (determined by automatic AA analysis) that included AspThrSerProGluGlyAlaValLeuTyrPheLysHisArg. FKII had an estimated molecular weight of 1000 with a 12-amino acid composition including AspSerProGluGlyAlaLeuTyrLysHisArg. The kinins were relatively rich in arginine and did not contain the bradykinin sequence.", "contents": "Isolation and partial characterization of kinin-like peptides formed by acid protease from murine fibroblast L-929. A kinin-forming acid protease was isolated and purified from a stationary cell culture of murine fibroblasts L-929 (Biochem. Pharmacol. 26: 1187, 1977). The enzyme formed kinins at an optimal pH of 4.0 from Murphy-Sturm lymphosarcoma tissue and rat plasma kininogen substrate. This study reports on the isolation and partial chemical characterization of two fibroblast kinins. The 12,000 g fraction of L-929 fibroblast homogenates was incubated with rat plasma at 37 degrees, pH 4.0, for 92 hours, and the kinins extracted with ethyl alcohol-p-toluene sulfonic acid. Two fibroblast kinins, FKI and FKII, were separated and purified on the following chromatographic columns: G-25 Sephadex (1 X 115 cm), CM-C50 Sephadex (2.5 X 15 cm), and Biogel P-4 (1 X 115 cm). Kinin activity was bioassayed on the perfused isolated rat uterus preparation. The purity of the kinins was ascertained by thin layer chromatography coupled with dansylation. The estimated molecular weight of FKI was 1450 with a 14-amino acid composition (determined by automatic AA analysis) that included AspThrSerProGluGlyAlaValLeuTyrPheLysHisArg. FKII had an estimated molecular weight of 1000 with a 12-amino acid composition including AspSerProGluGlyAlaLeuTyrLysHisArg. The kinins were relatively rich in arginine and did not contain the bradykinin sequence."}
{"id": "PMID:471942", "title": "Preparation and isolation of dityrosine.", "content": "A new procedure for the isolation of dityrosine has been developed. Tyrosine was oxidized by means of incubation both with hydrogen peroxide and horse-radish peroxidase. The reaction mixture was separated by permeation chromatography on Sephadex G-10 being monitored at 280 and 310 nm spectrophotometrically. The dityrosine fraction was freeze-dried and purified on a cation-exchange column (in acidic citrate buffer). The purified fraction was desalted and freeze-dried. The yield was 96 mg of homogenous dityrosine per 1 g of D,L-tyrosine. Some physico-chemical constants of the preparation were measured (optical characteristics with U.V. and I.R. spectra, fluorescence spectra, chromatography on an amino acid analyzer).", "contents": "Preparation and isolation of dityrosine. A new procedure for the isolation of dityrosine has been developed. Tyrosine was oxidized by means of incubation both with hydrogen peroxide and horse-radish peroxidase. The reaction mixture was separated by permeation chromatography on Sephadex G-10 being monitored at 280 and 310 nm spectrophotometrically. The dityrosine fraction was freeze-dried and purified on a cation-exchange column (in acidic citrate buffer). The purified fraction was desalted and freeze-dried. The yield was 96 mg of homogenous dityrosine per 1 g of D,L-tyrosine. Some physico-chemical constants of the preparation were measured (optical characteristics with U.V. and I.R. spectra, fluorescence spectra, chromatography on an amino acid analyzer)."}
{"id": "PMID:471943", "title": "Isolation of human C1q as a by-product of routine fractionation.", "content": "Clq can be isolated as a by-product of large scale fractionation. The euglobulins of fraction III are submitted to chromatography on CM-cellulose in a batch-procedure. The active fraction being frequently contaminated by lysozyme the protein can be further purified by gel-filtration in the presence of carbohydrates. The purified Clq is suitable for the detection of immune complexes.", "contents": "Isolation of human C1q as a by-product of routine fractionation. Clq can be isolated as a by-product of large scale fractionation. The euglobulins of fraction III are submitted to chromatography on CM-cellulose in a batch-procedure. The active fraction being frequently contaminated by lysozyme the protein can be further purified by gel-filtration in the presence of carbohydrates. The purified Clq is suitable for the detection of immune complexes."}
{"id": "PMID:471945", "title": "Preparation polyacrylamide-agarose gel electrophoresis of proteins and RNAs by the use of new device.", "content": "Quantitatively reproducible results were obtained by using a new device for preparation gel electrophoresis combined with polyacrylamide-agarose composite gel. When an adequate gel-buffer system was selected according to the procedure described in this paper, proteins and RNA's were well separated and recovered. The new device for preparative gel electrophoresis and the method for preparation of polyacrylamide-agarose composite gel are presented together with the elution profiles of the recovered substances.", "contents": "Preparation polyacrylamide-agarose gel electrophoresis of proteins and RNAs by the use of new device. Quantitatively reproducible results were obtained by using a new device for preparation gel electrophoresis combined with polyacrylamide-agarose composite gel. When an adequate gel-buffer system was selected according to the procedure described in this paper, proteins and RNA's were well separated and recovered. The new device for preparative gel electrophoresis and the method for preparation of polyacrylamide-agarose composite gel are presented together with the elution profiles of the recovered substances."}
{"id": "PMID:471946", "title": "Isolation, stabilization, and characterization of a toxin from timber rattlesnake venom.", "content": "A rapid and convenient method for the purification of a toxin from timber rattlesnake, Crotalus horridus horridus, venom using carboxymethyl cellulose ion-exchange chromatography has been devised. The toxicity of this venom component is labile, but it is stabilized by the addition of 20+ V/V glycerol to the buffer solution. This toxin has a molecular weight of 15,000 +/- 700 as determined by SDS gel electrophoresis. It is both heat and protease resistant. Treatment of this venom component with 2-mercaptoethanol followed by G-50 Sephadex chromatography causes no loss of toxicity although incubation of the toxin with 1% SDS and 1% 2-mercaptoethanol prior to electrophoresis does result in a faster migrating species. The toxin does not affect neuromuscular junctions but does appear to act on the nervous system. It causes no local responses in mice.", "contents": "Isolation, stabilization, and characterization of a toxin from timber rattlesnake venom. A rapid and convenient method for the purification of a toxin from timber rattlesnake, Crotalus horridus horridus, venom using carboxymethyl cellulose ion-exchange chromatography has been devised. The toxicity of this venom component is labile, but it is stabilized by the addition of 20+ V/V glycerol to the buffer solution. This toxin has a molecular weight of 15,000 +/- 700 as determined by SDS gel electrophoresis. It is both heat and protease resistant. Treatment of this venom component with 2-mercaptoethanol followed by G-50 Sephadex chromatography causes no loss of toxicity although incubation of the toxin with 1% SDS and 1% 2-mercaptoethanol prior to electrophoresis does result in a faster migrating species. The toxin does not affect neuromuscular junctions but does appear to act on the nervous system. It causes no local responses in mice."}
{"id": "PMID:471969", "title": "[Effect of radioactive iodine therapy and surgical treatment of thyrotoxicosis on the blood level of thyrotropin].", "content": "The radioimmunological method was applied to the study of the thyrotropic hormone (TTH) content in the blood of 308 patients with thyrotoxicosis at various stages of therapy with radioactive iodine and surgical treatment. After 131I treatment and elimination of the thyrotoxicosis symptoms TTH rose 1 to 2 years and 5 to 10 years after the onset of remission, this being regarded as an adaptive response to the changing thyroid activity. The hypothalamo-hypophysio-thyroid interaction was restored after the successful 131I therapy. However, surgical treatment of thyrothoxicosis did not lead to restoration of such interaction for 5 years--no changes of the TTH level followed either the \"primary\" subtotal thyroidectomy or repeated operation for the relapsing form of the disease.", "contents": "[Effect of radioactive iodine therapy and surgical treatment of thyrotoxicosis on the blood level of thyrotropin]. The radioimmunological method was applied to the study of the thyrotropic hormone (TTH) content in the blood of 308 patients with thyrotoxicosis at various stages of therapy with radioactive iodine and surgical treatment. After 131I treatment and elimination of the thyrotoxicosis symptoms TTH rose 1 to 2 years and 5 to 10 years after the onset of remission, this being regarded as an adaptive response to the changing thyroid activity. The hypothalamo-hypophysio-thyroid interaction was restored after the successful 131I therapy. However, surgical treatment of thyrothoxicosis did not lead to restoration of such interaction for 5 years--no changes of the TTH level followed either the \"primary\" subtotal thyroidectomy or repeated operation for the relapsing form of the disease."}
{"id": "PMID:471971", "title": "[Blood vasopressin concentration is patients of different ages with hypertension].", "content": "The concentration of blood vasopressin was investigated in apparently healthy persons and in patients with I--II degree hypertension, aged from 20 to 80 years. Vasopressin concentration was determined by the biological method according to the antidiuretic effect of ethanol-anesthetized and constantly hydrated rats on an original 5-channel apparatus. The results obtained showed the blood vasopressin concentration to increase with age. In patients with the I--II degree hypertensive disease the mentioned concentration was significantly higher than in healthy persons of the same age. Close correlation coefficient was revealed between the blood vasopressin concentration and minimal arterial blood pressure values.", "contents": "[Blood vasopressin concentration is patients of different ages with hypertension]. The concentration of blood vasopressin was investigated in apparently healthy persons and in patients with I--II degree hypertension, aged from 20 to 80 years. Vasopressin concentration was determined by the biological method according to the antidiuretic effect of ethanol-anesthetized and constantly hydrated rats on an original 5-channel apparatus. The results obtained showed the blood vasopressin concentration to increase with age. In patients with the I--II degree hypertensive disease the mentioned concentration was significantly higher than in healthy persons of the same age. Close correlation coefficient was revealed between the blood vasopressin concentration and minimal arterial blood pressure values."}
{"id": "PMID:471973", "title": "[Platelet aggregation in diabetes mellitus at various stages of development of retinopathy].", "content": "ADP-induced platelet aggregation was investigated in patients with diabetes mellitus at various stages of the development of retinopathy. Two parameters were used to evaluate the aggregation activity of platelets: the degree of aggregation and the dysaggregation percentage. There was revealed a direct correlation between the platelet hyperaggregation and the degree of diabetic retinopathy. A sharp elevation of the degree of aggregation and low dysaggregation percentage (up to its complete absence) were noted in patients with proliferating retinopathy; this could be supposed to be due to the supervention of the activating action of the altered vascular wall on the platelet aggregation. No reduction of platelet hyperaggregation under the effect of a brief treatment with such drugs as prodectin and dicion was revealed.", "contents": "[Platelet aggregation in diabetes mellitus at various stages of development of retinopathy]. ADP-induced platelet aggregation was investigated in patients with diabetes mellitus at various stages of the development of retinopathy. Two parameters were used to evaluate the aggregation activity of platelets: the degree of aggregation and the dysaggregation percentage. There was revealed a direct correlation between the platelet hyperaggregation and the degree of diabetic retinopathy. A sharp elevation of the degree of aggregation and low dysaggregation percentage (up to its complete absence) were noted in patients with proliferating retinopathy; this could be supposed to be due to the supervention of the activating action of the altered vascular wall on the platelet aggregation. No reduction of platelet hyperaggregation under the effect of a brief treatment with such drugs as prodectin and dicion was revealed."}
{"id": "PMID:471968", "title": "[Effect of angioprotectors on the glomerular filtration rate and vascular permeability in diabetic angiopathies].", "content": "Renal function (glomerular filtration) and vascular permeability under the effect of an angioprotector were studied by means of labeled renotropic compounds in 55 patients with diabetic angiopathies. The patients were divided into 3 groups: the first group comprised 23 patients treated with anginin or prodectin alone; the second one--16 patients given dicinon or docsium, and the third one--16 patient who were given combined treatment. The best effect was noted in the latter group of patients.", "contents": "[Effect of angioprotectors on the glomerular filtration rate and vascular permeability in diabetic angiopathies]. Renal function (glomerular filtration) and vascular permeability under the effect of an angioprotector were studied by means of labeled renotropic compounds in 55 patients with diabetic angiopathies. The patients were divided into 3 groups: the first group comprised 23 patients treated with anginin or prodectin alone; the second one--16 patients given dicinon or docsium, and the third one--16 patient who were given combined treatment. The best effect was noted in the latter group of patients."}
{"id": "PMID:471974", "title": "[Regulation of incorporation of amino acids into the thyroid gland proteins by thyroid hormones].", "content": "The effect of thyroid hormones and of iodide (1 x 10(-7)M, 5 x 10(-5)M) on the protein synthesis in the cell-free system made of the nodular goiter cells was investigated. As revealed, with the action of thyroid hormones the 14C-amino acids incorporation into the trichloracetic acid-precipitated proteins was considerably diminished. The greatest inhibition of the 14C-amino acids incorporation was observed in toxic (5 x 10(-5)M) hormone concentration. The inhibitory action of triiodthyronine was more expressed than that of thyroxin. Addition of potassium iodide into the incubation medium led to a slight inhibition of the labeled amino acids incorporation. The results obtained permit to regard thyroid hormones as highly specific inhibitors of protein synthesis in the thyroid gland.", "contents": "[Regulation of incorporation of amino acids into the thyroid gland proteins by thyroid hormones]. The effect of thyroid hormones and of iodide (1 x 10(-7)M, 5 x 10(-5)M) on the protein synthesis in the cell-free system made of the nodular goiter cells was investigated. As revealed, with the action of thyroid hormones the 14C-amino acids incorporation into the trichloracetic acid-precipitated proteins was considerably diminished. The greatest inhibition of the 14C-amino acids incorporation was observed in toxic (5 x 10(-5)M) hormone concentration. The inhibitory action of triiodthyronine was more expressed than that of thyroxin. Addition of potassium iodide into the incubation medium led to a slight inhibition of the labeled amino acids incorporation. The results obtained permit to regard thyroid hormones as highly specific inhibitors of protein synthesis in the thyroid gland."}
{"id": "PMID:471970", "title": "[Insulin and proinsulin content in the blood plasma in diabetes mellitus, determined by means of the gel filtration method].", "content": "With the aid of a simplified method of gel filtration the authors carried out separate determination of the immunoreactive insulin (IRI) and immunoreactive proinsulin (IRP) in 11 obese patients with diabetes mellitus and in 7 healthy persons during oral glucose tolerance test (GTT). In the presence of higher IRI, IRP, and general immunoreactivity (GIR) levels in patients in comparison with healthy persons the changes in IRI were similar to those of GIR in both groups: in healthy individuals the peak was observed 60, and in patients--120 minutes after glucose administration. A rise of IRP content occurred in both groups in 90 minutes, but in sick persons it was preceded by reduction of its level. In comparison with healthy individuals, the percentage of IRP from the sum total immunoreactivity was increased in the group of obese diabetes mellitus patients.", "contents": "[Insulin and proinsulin content in the blood plasma in diabetes mellitus, determined by means of the gel filtration method]. With the aid of a simplified method of gel filtration the authors carried out separate determination of the immunoreactive insulin (IRI) and immunoreactive proinsulin (IRP) in 11 obese patients with diabetes mellitus and in 7 healthy persons during oral glucose tolerance test (GTT). In the presence of higher IRI, IRP, and general immunoreactivity (GIR) levels in patients in comparison with healthy persons the changes in IRI were similar to those of GIR in both groups: in healthy individuals the peak was observed 60, and in patients--120 minutes after glucose administration. A rise of IRP content occurred in both groups in 90 minutes, but in sick persons it was preceded by reduction of its level. In comparison with healthy individuals, the percentage of IRP from the sum total immunoreactivity was increased in the group of obese diabetes mellitus patients."}
{"id": "PMID:471975", "title": "[Cytophotometric evaluation of the total protein content in the cell nucleus and cytoplasm of the hepatocytes in experimental hypothyroidism].", "content": "In rats with hypothyroid condition induced by bilateral subtotal thyroidectomy or mercazolyl feeding a comparative cytophotometric study of the protein content in the nuclei and the cytoplasm of hepatocytes and their area was carried out. The animals of both experimental groups displayed a marked reduction of the protein-synthesis function and also atrophy of the mentioned hepatocyte structures. However, in animals given mercazolyl these changes occurred earlier and were more pronounced, this indicating the combined effect of thyroid insufficiency and mercazolyl. Irrespective of the method of induction of the hypothyroid state (surgical or by drugs) disturbance of protein synthesis in the hepatocyte nuclei took place much earlier and was more intensive than in the cytoplasm.", "contents": "[Cytophotometric evaluation of the total protein content in the cell nucleus and cytoplasm of the hepatocytes in experimental hypothyroidism]. In rats with hypothyroid condition induced by bilateral subtotal thyroidectomy or mercazolyl feeding a comparative cytophotometric study of the protein content in the nuclei and the cytoplasm of hepatocytes and their area was carried out. The animals of both experimental groups displayed a marked reduction of the protein-synthesis function and also atrophy of the mentioned hepatocyte structures. However, in animals given mercazolyl these changes occurred earlier and were more pronounced, this indicating the combined effect of thyroid insufficiency and mercazolyl. Irrespective of the method of induction of the hypothyroid state (surgical or by drugs) disturbance of protein synthesis in the hepatocyte nuclei took place much earlier and was more intensive than in the cytoplasm."}
{"id": "PMID:471976", "title": "[Structural and cytochemical characteristics of the heart in the progeny of animals given mercazolyl during pregnancy and lactation].", "content": "Morphological and cytochemical features of the heart were studied in ratlings aged 1, 14, 21, 30, and 45 days born of animals which were given mercazolil (3 mg per 1 kg of body weight) during pregnancy and lactation. The relative heart weight of the progeny of the experimental animals proved to be considerably less during the postnatal period under study in comparison with control. A number of structural and cytochemical changes were displayed by the myocardium pointing to its dysfunction.", "contents": "[Structural and cytochemical characteristics of the heart in the progeny of animals given mercazolyl during pregnancy and lactation]. Morphological and cytochemical features of the heart were studied in ratlings aged 1, 14, 21, 30, and 45 days born of animals which were given mercazolil (3 mg per 1 kg of body weight) during pregnancy and lactation. The relative heart weight of the progeny of the experimental animals proved to be considerably less during the postnatal period under study in comparison with control. A number of structural and cytochemical changes were displayed by the myocardium pointing to its dysfunction."}
{"id": "PMID:471977", "title": "[Comparative evaluation of secretion of testicular androgens and excretion of their metabolites in young and old dogs].", "content": "It was shown experimentally that in old dogs a sharp reduction of testosterone and androstendion secretion by the testes was accompanied by a marked reduction of their metabolites (androsterone and ethiocholanolone) excretion. In the presence of a general reduction of the testicular androgen secretion and their urinary metabolite excretion there was noted a relative prevalence of fractions with low activity: of androstendion over testosterone in the blood, and of ethiocholanolone over androsterone in the urine. As postulated, in aged dogs hormonal background was characterized not only by quantitative, but also by qualitative changes, prerequisites for the development of visceral and metabolic disturbances.", "contents": "[Comparative evaluation of secretion of testicular androgens and excretion of their metabolites in young and old dogs]. It was shown experimentally that in old dogs a sharp reduction of testosterone and androstendion secretion by the testes was accompanied by a marked reduction of their metabolites (androsterone and ethiocholanolone) excretion. In the presence of a general reduction of the testicular androgen secretion and their urinary metabolite excretion there was noted a relative prevalence of fractions with low activity: of androstendion over testosterone in the blood, and of ethiocholanolone over androsterone in the urine. As postulated, in aged dogs hormonal background was characterized not only by quantitative, but also by qualitative changes, prerequisites for the development of visceral and metabolic disturbances."}
{"id": "PMID:471978", "title": "[Analytical ion-exchange and gel chromatography of radioisotope-labeled protein (polypeptide) hormones].", "content": "On the example of prolactin-I125 the author presents his experience in the use of gel filtration and ion-exchange chromatography for complete characterization of homogeneity and immunological properties of labeled hormones preparations. These methods can be used in studying various aspects of protein and polypeptide hormones metabolism, including various stages of their action mechanism, and also to characterize the hormones manufactured. It was shown that the formation of prolactin complex with gamma-globulins was accompanied by additional polarization of the hormone molecules, this leading to an increased energy of interaction with the ion exchange surface. The effect of polarization of the hormone molecules on the biological properties of the hormones themselves, their complexes with serum proteins and membrane receptors is discussed.", "contents": "[Analytical ion-exchange and gel chromatography of radioisotope-labeled protein (polypeptide) hormones]. On the example of prolactin-I125 the author presents his experience in the use of gel filtration and ion-exchange chromatography for complete characterization of homogeneity and immunological properties of labeled hormones preparations. These methods can be used in studying various aspects of protein and polypeptide hormones metabolism, including various stages of their action mechanism, and also to characterize the hormones manufactured. It was shown that the formation of prolactin complex with gamma-globulins was accompanied by additional polarization of the hormone molecules, this leading to an increased energy of interaction with the ion exchange surface. The effect of polarization of the hormone molecules on the biological properties of the hormones themselves, their complexes with serum proteins and membrane receptors is discussed."}
{"id": "PMID:472006", "title": "Nuclear resonant scattering of gamma rays--a new technique for in vivo measurement of body iron stores.", "content": "A technique for the measurement of body iron utilising nuclear resonant scattering of gamma rays has been developed. 847 keV photons emitted from a gaseous 56MnCl2 source are resonantly scattered from 56Fe present in the body. Measurement is made using large volume Ge(Li) detectors. The spatial uniformity of activation, the sensitivity of the detection system and the limits of detection have been investigated. Measurements were made on a liver phantom. The resonance scattering technique permits determination of normal levels of Fe in the liver with a radiation dose of 2 rem.", "contents": "Nuclear resonant scattering of gamma rays--a new technique for in vivo measurement of body iron stores. A technique for the measurement of body iron utilising nuclear resonant scattering of gamma rays has been developed. 847 keV photons emitted from a gaseous 56MnCl2 source are resonantly scattered from 56Fe present in the body. Measurement is made using large volume Ge(Li) detectors. The spatial uniformity of activation, the sensitivity of the detection system and the limits of detection have been investigated. Measurements were made on a liver phantom. The resonance scattering technique permits determination of normal levels of Fe in the liver with a radiation dose of 2 rem."}
{"id": "PMID:472007", "title": "Merit of Ge and Na(Tl) detectors as determined by imaging efficiency and scatter rejection.", "content": "Deep-lying cold lesions are often of marginal object contrast. Any contrast reduction caused by the detector may hinder their detectability. The capabilities of Ge and NaI(Tl) detectors are compared on the basis of imaging efficiency and scatter rejection capability. When the NaI(Tl) baseline is at 126 ke V (for 99Tcm), the void contrast obtainable with Ge detectors is 50% higher than that with NaI(Tl) using collimators having equally high resolution. If the signal-to-noise ratio (S/N) with Ge is not equal to or higher than that with NaI(Tl), this contrast advantage can be masked by excessive statistical noise. Since the lower detection efficiency of Ge crystals largely offsets their higher contrast, to obtain equal S/N in equal imaging times the Ge detection area must be comparable to that of NaI(Tl). When the NaI(Tl) baseline is at 145 keV, the window efficiency is reduced but the contrast is nearly as high as that with Ge. The Ge detection area for equal S/N is then only 40% of the NaI(Tl) area. Thus, a Ge detector having only half the area of NaI(Tl) is of questionable merit. Ge detector arrays comparable in area to NaI(Tl) are feasible and would clearly be superior imaging devices.", "contents": "Merit of Ge and Na(Tl) detectors as determined by imaging efficiency and scatter rejection. Deep-lying cold lesions are often of marginal object contrast. Any contrast reduction caused by the detector may hinder their detectability. The capabilities of Ge and NaI(Tl) detectors are compared on the basis of imaging efficiency and scatter rejection capability. When the NaI(Tl) baseline is at 126 ke V (for 99Tcm), the void contrast obtainable with Ge detectors is 50% higher than that with NaI(Tl) using collimators having equally high resolution. If the signal-to-noise ratio (S/N) with Ge is not equal to or higher than that with NaI(Tl), this contrast advantage can be masked by excessive statistical noise. Since the lower detection efficiency of Ge crystals largely offsets their higher contrast, to obtain equal S/N in equal imaging times the Ge detection area must be comparable to that of NaI(Tl). When the NaI(Tl) baseline is at 145 keV, the window efficiency is reduced but the contrast is nearly as high as that with Ge. The Ge detection area for equal S/N is then only 40% of the NaI(Tl) area. Thus, a Ge detector having only half the area of NaI(Tl) is of questionable merit. Ge detector arrays comparable in area to NaI(Tl) are feasible and would clearly be superior imaging devices."}
{"id": "PMID:472008", "title": "X-ray emission analysis of human plasma by proton excitation of thick samples in air.", "content": "Thick plasma samples have been irradiated in air by 2.3 MeV protons for trace elemental analysis by proton-induced X-ray emission spectroscopy. Accurate calibration curves were obtained for Fe, Zn and Sr and detection limits in dry plasma were estimated to be 0.08 PPM for Fe, 0.065 PPM for Zn and 0.12 PPM for Sr. Low temperature ashing of these samples improved the detection limits by a factor of 3 but reduced the precision and resulted in loss of bromine from the plasma. Mass absorption coefficients for low energy X-rays in dry plasma were measured and X-ray attenuation in the sample was found to prevent the detection of trace elements with Z less than 26. The method was used to determine Sr in plasma from a patient with bone disease, the Sr being a non-radioactive bone-seeking tracer element. The results, which were in the 3--13 PPM range, were in good general agreement with the analysis of the same samples by flame photometry.", "contents": "X-ray emission analysis of human plasma by proton excitation of thick samples in air. Thick plasma samples have been irradiated in air by 2.3 MeV protons for trace elemental analysis by proton-induced X-ray emission spectroscopy. Accurate calibration curves were obtained for Fe, Zn and Sr and detection limits in dry plasma were estimated to be 0.08 PPM for Fe, 0.065 PPM for Zn and 0.12 PPM for Sr. Low temperature ashing of these samples improved the detection limits by a factor of 3 but reduced the precision and resulted in loss of bromine from the plasma. Mass absorption coefficients for low energy X-rays in dry plasma were measured and X-ray attenuation in the sample was found to prevent the detection of trace elements with Z less than 26. The method was used to determine Sr in plasma from a patient with bone disease, the Sr being a non-radioactive bone-seeking tracer element. The results, which were in the 3--13 PPM range, were in good general agreement with the analysis of the same samples by flame photometry."}
{"id": "PMID:472009", "title": "Energy dependence of the neutron sensitivity of C--CO2, Mg--Ar and TE--TE ionisation chambers.", "content": "The neutron sensitivity relative to 60Co of commercially available C--CO2, Mg--Ar and TE--TE ionisation chambers was measured as a function of energy from 1 to 44 MeV. The sensitivity function was obtained by the method of Kuchnir, Vyborny and Skaggs from differences in measurements made at two angles in mixed fields having an isotropic gamma-ray component. Such fields were produced by bombardment of a thick beryllium target with 16 and 28 MeV deuterons, 44 MeV 3He-ions and 35 and 46 MeV protons. The results show that the relative neutron sensitivity of the C--CO2 and Mg--Ar chambers increases continuously with energy, whereas that of the TE--TE chamber is relatively constant.", "contents": "Energy dependence of the neutron sensitivity of C--CO2, Mg--Ar and TE--TE ionisation chambers. The neutron sensitivity relative to 60Co of commercially available C--CO2, Mg--Ar and TE--TE ionisation chambers was measured as a function of energy from 1 to 44 MeV. The sensitivity function was obtained by the method of Kuchnir, Vyborny and Skaggs from differences in measurements made at two angles in mixed fields having an isotropic gamma-ray component. Such fields were produced by bombardment of a thick beryllium target with 16 and 28 MeV deuterons, 44 MeV 3He-ions and 35 and 46 MeV protons. The results show that the relative neutron sensitivity of the C--CO2 and Mg--Ar chambers increases continuously with energy, whereas that of the TE--TE chamber is relatively constant."}
{"id": "PMID:472010", "title": "A modified lead attenuation method to determine the fast neutron sensitivity kU of a photon dosemeter.", "content": "Various lead attenuation techniques to determine the fast neutron sensitivity kU of a photon dosemeter are reviewed and a modified method is proposed to determine the kU value of a commercially available Geiger--M\u00fcller detector. The dependence on the 'effective' photon energy of the gamma-component of the mixed n--gamma field is illustrated and the most probable photon energy is deduced from measurements outside the radiation field. Using the relevant photon mass attenuation coefficient, measurements carried out with a well-collimated neutron beam at the MRC Cyclotron, Hammersmith Hospital, London, show that the Dg component contributes 2.41 +/- 0.13% to the total radiation field. Using a detector with a PTFE sleeve in the place of a Perspex sleeve, highly consistent results are obtained and the enhancement of the kU value by the Perspex is demonstrated. The neutron sensitivities of two MX 163 GM detectors with Perspex and PTFE sleeves were respectively determined as 0.73 +/- 0.07% and 0.98 +/- 0.14%.", "contents": "A modified lead attenuation method to determine the fast neutron sensitivity kU of a photon dosemeter. Various lead attenuation techniques to determine the fast neutron sensitivity kU of a photon dosemeter are reviewed and a modified method is proposed to determine the kU value of a commercially available Geiger--M\u00fcller detector. The dependence on the 'effective' photon energy of the gamma-component of the mixed n--gamma field is illustrated and the most probable photon energy is deduced from measurements outside the radiation field. Using the relevant photon mass attenuation coefficient, measurements carried out with a well-collimated neutron beam at the MRC Cyclotron, Hammersmith Hospital, London, show that the Dg component contributes 2.41 +/- 0.13% to the total radiation field. Using a detector with a PTFE sleeve in the place of a Perspex sleeve, highly consistent results are obtained and the enhancement of the kU value by the Perspex is demonstrated. The neutron sensitivities of two MX 163 GM detectors with Perspex and PTFE sleeves were respectively determined as 0.73 +/- 0.07% and 0.98 +/- 0.14%."}
{"id": "PMID:472011", "title": "The fast neutron sensitivity of a Geiger--M\u00fcller counter photon dosemeter by the time-of-flight technique.", "content": "The time-of-flight technique was used to measure the ratio of the sensitivity of an energy compensated Geiger--M\u00fcller counter dosemeter to 5 and 15.5 MeV neutrons, respectively, to its sensitivity to gamma rays (ku value).", "contents": "The fast neutron sensitivity of a Geiger--M\u00fcller counter photon dosemeter by the time-of-flight technique. The time-of-flight technique was used to measure the ratio of the sensitivity of an energy compensated Geiger--M\u00fcller counter dosemeter to 5 and 15.5 MeV neutrons, respectively, to its sensitivity to gamma rays (ku value)."}
{"id": "PMID:472012", "title": "Stripping of X-ray bremsstrahlung spectra up to 300 kVp on a desk type computer.", "content": "The direct result of a spectrometric measurement is a pulse height distribution. In the energy region up to 300 keV three corrections in particular need to be applied to get the photon spectrum: corrections for K-escape, Compton scattering and inefficient photon absorption. A simple 'stripping' procedure is described which may be implemented on a desk type computer. All data necessary are either available in the literature or may be derived from the measurement of very heavily filtered X-ray spectra. The accuracy of the procedure is better than +/- 5% of the peak value. Results are compared with a more detailed stripping procedure, based on Monte Carlo calculated data.", "contents": "Stripping of X-ray bremsstrahlung spectra up to 300 kVp on a desk type computer. The direct result of a spectrometric measurement is a pulse height distribution. In the energy region up to 300 keV three corrections in particular need to be applied to get the photon spectrum: corrections for K-escape, Compton scattering and inefficient photon absorption. A simple 'stripping' procedure is described which may be implemented on a desk type computer. All data necessary are either available in the literature or may be derived from the measurement of very heavily filtered X-ray spectra. The accuracy of the procedure is better than +/- 5% of the peak value. Results are compared with a more detailed stripping procedure, based on Monte Carlo calculated data."}
{"id": "PMID:472013", "title": "Image formation by back-projection: a reappraisal.", "content": "The theory of image formation by simple summation of an object's projections, without further processing, is considered. Calculations are presented of the resultant image from a point, a rectangle, an annulus, and three rotationally symmetric objects. The general features of these images are discussed and it is postulated that for many objects of interest a limited compensation for edge unsharpening would suffice to produce recognition of the objects.", "contents": "Image formation by back-projection: a reappraisal. The theory of image formation by simple summation of an object's projections, without further processing, is considered. Calculations are presented of the resultant image from a point, a rectangle, an annulus, and three rotationally symmetric objects. The general features of these images are discussed and it is postulated that for many objects of interest a limited compensation for edge unsharpening would suffice to produce recognition of the objects."}
{"id": "PMID:472014", "title": "The direct use of CT numbers in radiotherapy dosage calculations for inhomogeneous media.", "content": "Techniques by which the quantitative anatomical data inherent in a CT scan can be directly used in treatment planning are described. The correction algorithms used in the RAD-8 system, based on an effective path length, have been extended to a pixel-by-pixel approach. By calibrating the X-ray transmission CT scanner in terms of electron densities (electron cm-3) inhomogeneity corrections may be made automatically.", "contents": "The direct use of CT numbers in radiotherapy dosage calculations for inhomogeneous media. Techniques by which the quantitative anatomical data inherent in a CT scan can be directly used in treatment planning are described. The correction algorithms used in the RAD-8 system, based on an effective path length, have been extended to a pixel-by-pixel approach. By calibrating the X-ray transmission CT scanner in terms of electron densities (electron cm-3) inhomogeneity corrections may be made automatically."}
{"id": "PMID:472023", "title": "Reflexes evoked by group II afferent fibers from muscle spindles.", "content": "The purpose of this paper is to review research on the reflex action of group II afferent fibers originating in the muscle spindle. Although the reflex actions of the Ia afferent fibers from muscle spindles are well defined, the reflex action of the group II afferent fibers is not clearly understood. Experiments on animals whose spinal cord had been transected gave rise to the idea that group II afferent fibers facilitate flexor muscles and inhibit extensor muscles regardless of the origin of the fibers. This concept has been accepted by neurophysiologists and has even been incorporated into therapeutic exercise procedures by physical therapists. However, accumulating evidence is presented that challenges this classical concept. Physical therapists, when trying to incorporate the reflexes evoked by the group II afferent fibers into treatment, must become better aware of the complex reflex actions of which these fibers are capable.", "contents": "Reflexes evoked by group II afferent fibers from muscle spindles. The purpose of this paper is to review research on the reflex action of group II afferent fibers originating in the muscle spindle. Although the reflex actions of the Ia afferent fibers from muscle spindles are well defined, the reflex action of the group II afferent fibers is not clearly understood. Experiments on animals whose spinal cord had been transected gave rise to the idea that group II afferent fibers facilitate flexor muscles and inhibit extensor muscles regardless of the origin of the fibers. This concept has been accepted by neurophysiologists and has even been incorporated into therapeutic exercise procedures by physical therapists. However, accumulating evidence is presented that challenges this classical concept. Physical therapists, when trying to incorporate the reflexes evoked by the group II afferent fibers into treatment, must become better aware of the complex reflex actions of which these fibers are capable."}
{"id": "PMID:472024", "title": "Competency: the what, why, and how of it.", "content": "Competency is a frequently used term in the physical therapy profession, but many physical therapists are unclear about its definition and its meaning in professional activity. This article describes the operational definition of competency as used in the practice of physical therapy by members of the Association; describes the development of the manual, Competencies in Physical Therapy: An Analysis of Practice; offers direction in possible uses of the manual; and suggests future uses for the manual in Association activity.", "contents": "Competency: the what, why, and how of it. Competency is a frequently used term in the physical therapy profession, but many physical therapists are unclear about its definition and its meaning in professional activity. This article describes the operational definition of competency as used in the practice of physical therapy by members of the Association; describes the development of the manual, Competencies in Physical Therapy: An Analysis of Practice; offers direction in possible uses of the manual; and suggests future uses for the manual in Association activity."}
{"id": "PMID:472025", "title": "Salary and status differences between male and female physical therapists.", "content": "The purpose of this study was to compare employment profiles of men and women employed as physical therapists. A survey on salary and on demographic and professional characteristics was mailed to 500 male and 500 female physical therapists throughout the United States, and 658 (65.8%) were used in the data analysis. Several striking differences between the employment profiles of male and female physical therapists were identified, including: 1) men were more likely to be self-employed, 2) men were more likely to hold supervisory positions, and 3) men tended to have significantly higher incomes even when compared to women with similar employment characteristics. The salary and status differences between men and women in physical therapy approximate those found in other occupations in which women are in the majority. The findings of this study constitute strong evidence that sex discrimination exists in the physical therapy profession.", "contents": "Salary and status differences between male and female physical therapists. The purpose of this study was to compare employment profiles of men and women employed as physical therapists. A survey on salary and on demographic and professional characteristics was mailed to 500 male and 500 female physical therapists throughout the United States, and 658 (65.8%) were used in the data analysis. Several striking differences between the employment profiles of male and female physical therapists were identified, including: 1) men were more likely to be self-employed, 2) men were more likely to hold supervisory positions, and 3) men tended to have significantly higher incomes even when compared to women with similar employment characteristics. The salary and status differences between men and women in physical therapy approximate those found in other occupations in which women are in the majority. The findings of this study constitute strong evidence that sex discrimination exists in the physical therapy profession."}
{"id": "PMID:472026", "title": "Structure of ethics teaching in physical therapy: a survey.", "content": "Courses called \"Ethics\" have long been included in physical therapy education programs. This paper reports the findings of a survey designed to assess current practices in teaching ethics in baccalaureate-level physical therapy programs. Of 59 questionnaires sent, 29 were returned and analyzed. The findings are compared with similar recent surveys of medical and nursing schools. Suggestions are made for assuring that the high standard of teaching maintained in other areas of physical therapy professional preparation also are maintained in ethics teaching.", "contents": "Structure of ethics teaching in physical therapy: a survey. Courses called \"Ethics\" have long been included in physical therapy education programs. This paper reports the findings of a survey designed to assess current practices in teaching ethics in baccalaureate-level physical therapy programs. Of 59 questionnaires sent, 29 were returned and analyzed. The findings are compared with similar recent surveys of medical and nursing schools. Suggestions are made for assuring that the high standard of teaching maintained in other areas of physical therapy professional preparation also are maintained in ethics teaching."}
{"id": "PMID:472027", "title": "Improved ambulation in a hemiplegic patient following phenol block.", "content": "Within one week after the phenol block, the patient made significant functional gains in areas in which he had previously reached a plateau. He progressed in walking from needing assistance to being independent. Although he still required an orthosis and an ambulation aid, his need for them was greatly reduced. Follow-up, both after discharge and approximately three months after having the phenol block, revealed good results. The patient had maintained his highest functional level as an independent community ambulator.", "contents": "Improved ambulation in a hemiplegic patient following phenol block. Within one week after the phenol block, the patient made significant functional gains in areas in which he had previously reached a plateau. He progressed in walking from needing assistance to being independent. Although he still required an orthosis and an ambulation aid, his need for them was greatly reduced. Follow-up, both after discharge and approximately three months after having the phenol block, revealed good results. The patient had maintained his highest functional level as an independent community ambulator."}
{"id": "PMID:472040", "title": "One preventable cause of spontaneous deflation of inflatable mammary prostheses.", "content": "Spontaneous \"overnight\" deflation of inflatable prostheses is rather uncommon, but we have had a 5.7% incidence of it in a 24-month period in which we used implants with a suturable tab and fastened them to the subjacent fascia. At exploration we found these tabs had torn the bag of the implant, usually at the vulcanized seam. We now believe that fixing a suture tab on a breast implant to underlying fascia may cause undue stresses upon the implant at that point and result in an otherwise avoidable deflation.", "contents": "One preventable cause of spontaneous deflation of inflatable mammary prostheses. Spontaneous \"overnight\" deflation of inflatable prostheses is rather uncommon, but we have had a 5.7% incidence of it in a 24-month period in which we used implants with a suturable tab and fastened them to the subjacent fascia. At exploration we found these tabs had torn the bag of the implant, usually at the vulcanized seam. We now believe that fixing a suture tab on a breast implant to underlying fascia may cause undue stresses upon the implant at that point and result in an otherwise avoidable deflation."}
{"id": "PMID:472041", "title": "Correction of major defects of the vermilion with a cross-lip vermilion flap.", "content": "A method is described to correct major vermilion defects by using a transverse, cross-lip, vermilion flap. Sizable defects can be easily filled in to obtain an upper lip with better contour and simultaneously reduce the unpleasant fullness of the lower lip to produce better balance between the two lips.", "contents": "Correction of major defects of the vermilion with a cross-lip vermilion flap. A method is described to correct major vermilion defects by using a transverse, cross-lip, vermilion flap. Sizable defects can be easily filled in to obtain an upper lip with better contour and simultaneously reduce the unpleasant fullness of the lower lip to produce better balance between the two lips."}
{"id": "PMID:472042", "title": "Results of the Matev operation for correction of Boutonni\u00e8re deformity.", "content": "Between December 1971 and December 1977, 41 patients with long-standing boutonni\u00e8re deformities were treated by Matev's operation. The average follow-up time was 13 months (range 8 to 18 months). The duration of their lesions varied from 32 to 176 days. The digits most affected were the long finger (14) and the ring finger (11). Postoperatively, all patients showed excellent flexion of the fingers with only a very slight deficit in extension of the PIP joint. Eight of them also had some deficit in extension of the DIP joint, with flexion there of 10 degrees to 15 degrees, but little real disability. The results indicate that when the Matev operation is clearly indicated and properly performed, it is an excellent one for the treatment of this condition.", "contents": "Results of the Matev operation for correction of Boutonni\u00e8re deformity. Between December 1971 and December 1977, 41 patients with long-standing boutonni\u00e8re deformities were treated by Matev's operation. The average follow-up time was 13 months (range 8 to 18 months). The duration of their lesions varied from 32 to 176 days. The digits most affected were the long finger (14) and the ring finger (11). Postoperatively, all patients showed excellent flexion of the fingers with only a very slight deficit in extension of the PIP joint. Eight of them also had some deficit in extension of the DIP joint, with flexion there of 10 degrees to 15 degrees, but little real disability. The results indicate that when the Matev operation is clearly indicated and properly performed, it is an excellent one for the treatment of this condition."}
{"id": "PMID:472043", "title": "Rerouting vessels and nerves from other digits in replanting an avulsed and degloved thumb.", "content": "In avulsion and degloving injuries of amputated thumbs, we have rotated nearby neurovascular bundles and dorsal digital vessels to restore circulation and sensation. This has been done in 5 patients, and 3 thumbs survived with preservation of length and good function.", "contents": "Rerouting vessels and nerves from other digits in replanting an avulsed and degloved thumb. In avulsion and degloving injuries of amputated thumbs, we have rotated nearby neurovascular bundles and dorsal digital vessels to restore circulation and sensation. This has been done in 5 patients, and 3 thumbs survived with preservation of length and good function."}
{"id": "PMID:472044", "title": "Experimental use of free gastric flaps for the repair of pharyngoesophageal defects.", "content": "The repair of large pharyngoesophageal defects was accomplished experimentally in 16 dogs with revascularized free flaps from the greater curvature of the stomach. These flaps were based on the gastroepiploic vessels, and they were anastomosed to the carotid artery and external jugular vein in the neck. The procedure had a low mortality and did not lead to peptic ulceration or hyperchlorhydria in these animals.", "contents": "Experimental use of free gastric flaps for the repair of pharyngoesophageal defects. The repair of large pharyngoesophageal defects was accomplished experimentally in 16 dogs with revascularized free flaps from the greater curvature of the stomach. These flaps were based on the gastroepiploic vessels, and they were anastomosed to the carotid artery and external jugular vein in the neck. The procedure had a low mortality and did not lead to peptic ulceration or hyperchlorhydria in these animals."}
{"id": "PMID:472046", "title": "Capsular contracture and steroid-related complications after augmentation mammaplasty. A preliminary study.", "content": "Ninety-one patients who had had augmentation mammaplasties were evaluated retrospectively. The intra-implant use of steroids appeared to reduce the incidence of breast firmness--at least over the short follow-up period--but there were complications from this which appear to be dose related (mg of Solu-Medrol/100 cc saline). A long-term evaluation of such patients is desirable. Meanwhile, our limited data strongly suggest that the intra-implant use of steroids should be used only with caution as to the dose and with a careful follow-up.", "contents": "Capsular contracture and steroid-related complications after augmentation mammaplasty. A preliminary study. Ninety-one patients who had had augmentation mammaplasties were evaluated retrospectively. The intra-implant use of steroids appeared to reduce the incidence of breast firmness--at least over the short follow-up period--but there were complications from this which appear to be dose related (mg of Solu-Medrol/100 cc saline). A long-term evaluation of such patients is desirable. Meanwhile, our limited data strongly suggest that the intra-implant use of steroids should be used only with caution as to the dose and with a careful follow-up."}
{"id": "PMID:472057", "title": "The omental sandwich reconstruction for a full-thickness cheek defect.", "content": "We present a case in which a large full-thickness cheek defect was successfully repaired in one stage by the \"omental sandwich technique.\" We transferred the omentum as a free flap, anastomosing the middle omental vessels with the end stumps of the gastroepiploic vessels after they had been brought to the neck through a subcutaneous tunnel. Skin grafts were applied then on both sides of the revascularized omentum.", "contents": "The omental sandwich reconstruction for a full-thickness cheek defect. We present a case in which a large full-thickness cheek defect was successfully repaired in one stage by the \"omental sandwich technique.\" We transferred the omentum as a free flap, anastomosing the middle omental vessels with the end stumps of the gastroepiploic vessels after they had been brought to the neck through a subcutaneous tunnel. Skin grafts were applied then on both sides of the revascularized omentum."}
{"id": "PMID:472058", "title": "Hand replantation in an 83-year-old woman--the oldest replantation?", "content": "A replantation of the hand was performed in an 83-year-old woman. There was good survival and the early functional return was satisfactory. However, after a personal tragedy and after the physiotherapy slowed down there was rapid deterioration in her hand function. Disuse atrophy became so marked after two years that the replanted hand became a stiff organ with little function.", "contents": "Hand replantation in an 83-year-old woman--the oldest replantation? A replantation of the hand was performed in an 83-year-old woman. There was good survival and the early functional return was satisfactory. However, after a personal tragedy and after the physiotherapy slowed down there was rapid deterioration in her hand function. Disuse atrophy became so marked after two years that the replanted hand became a stiff organ with little function."}
{"id": "PMID:472059", "title": "Use of Trendelenburg position to reveal open blood vessels and prevent hematomas in face lifts.", "content": "We have had a substantial decrease in the number of postoperative hematomas following face lifts since we started putting the patients in extreme Trendelenburg position after all the cutting was done, to reveal any potential bleeding points. These are then coagulated or ligated to obtain more complete hemostasis.", "contents": "Use of Trendelenburg position to reveal open blood vessels and prevent hematomas in face lifts. We have had a substantial decrease in the number of postoperative hematomas following face lifts since we started putting the patients in extreme Trendelenburg position after all the cutting was done, to reveal any potential bleeding points. These are then coagulated or ligated to obtain more complete hemostasis."}
{"id": "PMID:472071", "title": "Physiological and pharmacological basis for the chemotherapy of enuresis.", "content": "Enuresis is a disorder of micturition occuring in the absence of an organic urinary tract lesion. To understand its possible causation, the mechanisms controlling micturition are described together with the possible sites of action of various anti-enuretic agents, particularly imipramine. It is concluded that further research into the central control of micturition is required before the precise actions of centrally-acting anti-enuretic agents can be elucidated. Knowledge of these may give insight into the nature of the defect causing enuresis.", "contents": "Physiological and pharmacological basis for the chemotherapy of enuresis. Enuresis is a disorder of micturition occuring in the absence of an organic urinary tract lesion. To understand its possible causation, the mechanisms controlling micturition are described together with the possible sites of action of various anti-enuretic agents, particularly imipramine. It is concluded that further research into the central control of micturition is required before the precise actions of centrally-acting anti-enuretic agents can be elucidated. Knowledge of these may give insight into the nature of the defect causing enuresis."}
{"id": "PMID:472072", "title": "The Eating Attitudes Test: an index of the symptoms of anorexia nervosa.", "content": "Data on the development of a 40-item measure of the symptoms in anorexia nervosa are reported. The scale (EAT) is presented in a 6-point, forced choice, self-report format which is easily administered and scored. The EAT was validated using 2 groups of female anorexia nervosa patients (N = 32 and 33) and female control subjects (N = 34 and 59). Total EAT score was significantly correlated with criterion group membership (r = 0.87, P less than 0.001), suggesting a high level of concurrent validity. There was very little overlap in the frequency distributions of the 2 groups and only 7% of the normal controls scored as high as the lowest anorexic patient. Female obese and male subjects also scored significantly lower on the EAT than anorexics. Recovered anorexic patients scored in the normal range on the test, suggesting that the EAT is sensitive to clinical remission.", "contents": "The Eating Attitudes Test: an index of the symptoms of anorexia nervosa. Data on the development of a 40-item measure of the symptoms in anorexia nervosa are reported. The scale (EAT) is presented in a 6-point, forced choice, self-report format which is easily administered and scored. The EAT was validated using 2 groups of female anorexia nervosa patients (N = 32 and 33) and female control subjects (N = 34 and 59). Total EAT score was significantly correlated with criterion group membership (r = 0.87, P less than 0.001), suggesting a high level of concurrent validity. There was very little overlap in the frequency distributions of the 2 groups and only 7% of the normal controls scored as high as the lowest anorexic patient. Female obese and male subjects also scored significantly lower on the EAT than anorexics. Recovered anorexic patients scored in the normal range on the test, suggesting that the EAT is sensitive to clinical remission."}
{"id": "PMID:472073", "title": "The effect of temporal lobe surgery on electrodermal activity: implications for an organic hypothesis in the aetiology of schizophrenia.", "content": "It has recently been suggested that the bilateral asymmetry of electrodermal activity (EDA) reported in schizophrenia may be related to unilateral temporal lobe dysfunction. To test this hypothesis, 3 aspects of EDA--skin conductance level (SCL), number of spontaneous fluctuations (SF), and skin conductance response (SCR)--were measured bilaterally in 10 patients who had undergone unilateral temporal lobectomy. No differences could be detected between the operated and non-operated sides within the patient group, nor between the patient and control groups. The implications of these findings are discussed.", "contents": "The effect of temporal lobe surgery on electrodermal activity: implications for an organic hypothesis in the aetiology of schizophrenia. It has recently been suggested that the bilateral asymmetry of electrodermal activity (EDA) reported in schizophrenia may be related to unilateral temporal lobe dysfunction. To test this hypothesis, 3 aspects of EDA--skin conductance level (SCL), number of spontaneous fluctuations (SF), and skin conductance response (SCR)--were measured bilaterally in 10 patients who had undergone unilateral temporal lobectomy. No differences could be detected between the operated and non-operated sides within the patient group, nor between the patient and control groups. The implications of these findings are discussed."}
{"id": "PMID:472074", "title": "Follow-up of 11 XYY males with impulsive and/or sex-offending behaviour.", "content": "Eleven behaviourally abnormal XYY males who had been treated in a structured combined programme of antiandrogen medication and counseling were followed up 1 year after cessation of the programme. Behavioural ratings were made in each of 5 categories: assault against people, destructiveness against things, threatening behaviour, stealing, and self-harming. On examining the follow-up findings, therapeutic success appeared questionable.", "contents": "Follow-up of 11 XYY males with impulsive and/or sex-offending behaviour. Eleven behaviourally abnormal XYY males who had been treated in a structured combined programme of antiandrogen medication and counseling were followed up 1 year after cessation of the programme. Behavioural ratings were made in each of 5 categories: assault against people, destructiveness against things, threatening behaviour, stealing, and self-harming. On examining the follow-up findings, therapeutic success appeared questionable."}
{"id": "PMID:472075", "title": "Hysterical traits and variability of mood in normal men.", "content": "In a study of 40 normal men it was found that self-ratings on variability of mood were positively correlated with self-ratings on hysterical traits. These results are similar to those found in normal women and lend support both to the validity of the concept of hysterical personality and to the idea that men and women experience fluctuations of mood in a similar way.", "contents": "Hysterical traits and variability of mood in normal men. In a study of 40 normal men it was found that self-ratings on variability of mood were positively correlated with self-ratings on hysterical traits. These results are similar to those found in normal women and lend support both to the validity of the concept of hysterical personality and to the idea that men and women experience fluctuations of mood in a similar way."}
{"id": "PMID:472076", "title": "The Leyton Obsessional Inventory: an analysis of the responses of 73 obsessional patients.", "content": "The Leyton Obsessional Inventory was administered to 73 obsessive-compulsive neurotics, and their responses compared with those of 100 normal subjects. The ratio of the mean patient to normal scores ranged from 2.4:1 for obsessional traits and 3.2:1 for symptoms to 6.2:1 for resistance and 12.5:1 for interference with other activities. A principal components analysis on the patients' replies produced 3 unitary components (household order, personal contamination, and doubting) plus 2 bipolar components (checking/parsimony and desire for closure/unpleasant ruminations). These appeared to be more definitive representations of components identified from a similar analysis on normal subjects, suggesting that obsessional neurotics differ from normal subjects quantitatively rather than qualitatively. A cluster analysis on the patients' responses produced 3 subgroups. Thirty-two patients were predominantly hesitant and indecisive ('doubters'), 30 were concerned with bodily and clothing contamination ('contaminators'), and 7 were preoccupied with checking ('checkers').", "contents": "The Leyton Obsessional Inventory: an analysis of the responses of 73 obsessional patients. The Leyton Obsessional Inventory was administered to 73 obsessive-compulsive neurotics, and their responses compared with those of 100 normal subjects. The ratio of the mean patient to normal scores ranged from 2.4:1 for obsessional traits and 3.2:1 for symptoms to 6.2:1 for resistance and 12.5:1 for interference with other activities. A principal components analysis on the patients' replies produced 3 unitary components (household order, personal contamination, and doubting) plus 2 bipolar components (checking/parsimony and desire for closure/unpleasant ruminations). These appeared to be more definitive representations of components identified from a similar analysis on normal subjects, suggesting that obsessional neurotics differ from normal subjects quantitatively rather than qualitatively. A cluster analysis on the patients' responses produced 3 subgroups. Thirty-two patients were predominantly hesitant and indecisive ('doubters'), 30 were concerned with bodily and clothing contamination ('contaminators'), and 7 were preoccupied with checking ('checkers')."}
{"id": "PMID:472077", "title": "Application to the alcohol dependence syndrome of a method of determining the sequential development of symptoms.", "content": "Temporal ordering of core items in the alcohol dependence syndrome was investigated in 38 men admitted to an alcoholism treatment unit. An analysis of rank sums yielded a modal sequence which resembled classical descriptions of alcoholism, though 'loss of control' appeared very early.", "contents": "Application to the alcohol dependence syndrome of a method of determining the sequential development of symptoms. Temporal ordering of core items in the alcohol dependence syndrome was investigated in 38 men admitted to an alcoholism treatment unit. An analysis of rank sums yielded a modal sequence which resembled classical descriptions of alcoholism, though 'loss of control' appeared very early."}
{"id": "PMID:472078", "title": "Classification of depression and response to amitriptyline therapy.", "content": "Fifty-four patients suffering from primary depressive illness were rated on the Newcastle diagnostic scale while taking part in a pharmacokinetic study of amitriptyline therapy, and their clinical response was assessed by the Hamilton Rating Scale for depression. Patients with a Newcastle score of 4-8 showed the best response to amitriptyline. Patients with low Newcastle scores, representing the non-endogenous or neurotic group, responded poorly. Patients with high scores on the Newcastle scale, representing those with marked endogenous features, also responded poorly.", "contents": "Classification of depression and response to amitriptyline therapy. Fifty-four patients suffering from primary depressive illness were rated on the Newcastle diagnostic scale while taking part in a pharmacokinetic study of amitriptyline therapy, and their clinical response was assessed by the Hamilton Rating Scale for depression. Patients with a Newcastle score of 4-8 showed the best response to amitriptyline. Patients with low Newcastle scores, representing the non-endogenous or neurotic group, responded poorly. Patients with high scores on the Newcastle scale, representing those with marked endogenous features, also responded poorly."}
{"id": "PMID:472079", "title": "Determinants of the ability of general practitioners to detect psychiatric illness.", "content": "This study of psychiatric illness among 4098 patients attending 91 general practitioners compares 2 methods of case identification: 'conspicuous morbidity' by the doctor's own assessments, and 'probable prevalence' by the patients' responses to the General Health Questionnaire (GHQ). In general, the latter gives somewhat higher estimates than the former, but there are wide variations in morbidity between practices. The ability of each general practitioner to detect psychiatric illness was measured by computing Spearman's correlation coefficient between his assessments and the GHQ scores of his patients. The mean correlation coefficient was + 0.36, but the range was very wide (0.09-0.60). The first part of the study deals with various demographic characteristics of the patients themselves which are associated with an increased likelihood of the doctor detecting a psychiatric illness; such factors include unemployment, female sex, and marriages which have ended by separation, divorce or death. The second part of the study examines characteristics of the doctors themselves in an attempt to account for the wide variation between them in their ability to detect psychiatric illness. A research psychiatrist made detailed observations on 2098 interviews carried out by 55 general practitioners. Each doctor's verbal and non-verbal styles were recorded minutely, and in addition various global ratings were made. The doctors completed personality inventories and supplied details of training and professional background. It was possible to account for 67% of the variance of correlation coefficient mainly in terms of 2 dimensions: 'interest and concern' and 'conservatism'. The way in which the doctor interviews his patients is shown to be important, but there are interactions between interview style and the doctor's personality.", "contents": "Determinants of the ability of general practitioners to detect psychiatric illness. This study of psychiatric illness among 4098 patients attending 91 general practitioners compares 2 methods of case identification: 'conspicuous morbidity' by the doctor's own assessments, and 'probable prevalence' by the patients' responses to the General Health Questionnaire (GHQ). In general, the latter gives somewhat higher estimates than the former, but there are wide variations in morbidity between practices. The ability of each general practitioner to detect psychiatric illness was measured by computing Spearman's correlation coefficient between his assessments and the GHQ scores of his patients. The mean correlation coefficient was + 0.36, but the range was very wide (0.09-0.60). The first part of the study deals with various demographic characteristics of the patients themselves which are associated with an increased likelihood of the doctor detecting a psychiatric illness; such factors include unemployment, female sex, and marriages which have ended by separation, divorce or death. The second part of the study examines characteristics of the doctors themselves in an attempt to account for the wide variation between them in their ability to detect psychiatric illness. A research psychiatrist made detailed observations on 2098 interviews carried out by 55 general practitioners. Each doctor's verbal and non-verbal styles were recorded minutely, and in addition various global ratings were made. The doctors completed personality inventories and supplied details of training and professional background. It was possible to account for 67% of the variance of correlation coefficient mainly in terms of 2 dimensions: 'interest and concern' and 'conservatism'. The way in which the doctor interviews his patients is shown to be important, but there are interactions between interview style and the doctor's personality."}
{"id": "PMID:472080", "title": "Variations in referral pattern to the psychiatric services by general practitioners.", "content": "Referral rates to psychiatric services in North-East Scotland by 147 individual urban and rural general practitioners were calculated. Among city doctors, the greater the number of years since qualification the more patients were referred. Single-handed city doctors also had very high referral rates. Subsequent interviews were conducted with 76 general practitioners. Topics covered included reasons for referral to psychiatric services. Psychotics, severely depressed patients, alcoholics and violent patients were the most frequently referred groups.", "contents": "Variations in referral pattern to the psychiatric services by general practitioners. Referral rates to psychiatric services in North-East Scotland by 147 individual urban and rural general practitioners were calculated. Among city doctors, the greater the number of years since qualification the more patients were referred. Single-handed city doctors also had very high referral rates. Subsequent interviews were conducted with 76 general practitioners. Topics covered included reasons for referral to psychiatric services. Psychotics, severely depressed patients, alcoholics and violent patients were the most frequently referred groups."}
{"id": "PMID:472081", "title": "Informing the consultant psychiatrist--the problems of communication.", "content": "There is considerable difficulty in providing clinicians with statistics relating to their work which are both relevant in content and suitable in style. As a contribution towards the solution of the problem for psychiatrists, a new type of statistical feedback, the Hospital/National Comparison Tables, has been produced in Scotland. In these tables the work of individual hospitals is compared with the work of all other hospitals of the same type. These are critically examined, together with examples of analyses showing the uses to which the data can be put.", "contents": "Informing the consultant psychiatrist--the problems of communication. There is considerable difficulty in providing clinicians with statistics relating to their work which are both relevant in content and suitable in style. As a contribution towards the solution of the problem for psychiatrists, a new type of statistical feedback, the Hospital/National Comparison Tables, has been produced in Scotland. In these tables the work of individual hospitals is compared with the work of all other hospitals of the same type. These are critically examined, together with examples of analyses showing the uses to which the data can be put."}
{"id": "PMID:472082", "title": "Genetic and physical studies of male children with psychological gender disturbances.", "content": "Twelve male children were diagnosed with psychological gender disturbances by 3 independent clinical psychologists using independent data sources focusing on behavioural deviance from  normal comparison groups, on conventional psychological testing, and on parent report instruments which had been validated on normal comparison samples. These children received a paediatric evaluation consisting of a medical history, complete physical examination, chromosome analysis including 2 cells karyotyped and 15 counted, and sex chromatin studies. All gender disturbed boys were found to be normal genetically and physically with the exception of one subject with one undescended testicle.", "contents": "Genetic and physical studies of male children with psychological gender disturbances. Twelve male children were diagnosed with psychological gender disturbances by 3 independent clinical psychologists using independent data sources focusing on behavioural deviance from  normal comparison groups, on conventional psychological testing, and on parent report instruments which had been validated on normal comparison samples. These children received a paediatric evaluation consisting of a medical history, complete physical examination, chromosome analysis including 2 cells karyotyped and 15 counted, and sex chromatin studies. All gender disturbed boys were found to be normal genetically and physically with the exception of one subject with one undescended testicle."}
{"id": "PMID:472083", "title": "Tranylcypromine ('Parnate') overdose: measurement of tranylcypromine concentrations and MAO inhibitory activity and identification of amphetamines in plasma.", "content": "A case of tranylcypromine overdose is reported. Tranylcypromine, amphetamine methamphetamine and phenylethylamine were detected in the plasma by gas chromatography and their identity confirmed by mass spectrometry. The data suggested that the amphetamines were metabolic products of tranylcypromine. Platelet monoamine oxidase activity was more than 95% inhibited during the 72 h after the overdose despite complete clinical recovery by that time. The possible role of amphetamines and phenylethylamine in causing the clinical manifestations of tranylcypromine overdose is discussed.", "contents": "Tranylcypromine ('Parnate') overdose: measurement of tranylcypromine concentrations and MAO inhibitory activity and identification of amphetamines in plasma. A case of tranylcypromine overdose is reported. Tranylcypromine, amphetamine methamphetamine and phenylethylamine were detected in the plasma by gas chromatography and their identity confirmed by mass spectrometry. The data suggested that the amphetamines were metabolic products of tranylcypromine. Platelet monoamine oxidase activity was more than 95% inhibited during the 72 h after the overdose despite complete clinical recovery by that time. The possible role of amphetamines and phenylethylamine in causing the clinical manifestations of tranylcypromine overdose is discussed."}
{"id": "PMID:472084", "title": "Overachievement and catecholamine excretion in an achievement-demanding situation.", "content": "We have studied the relationship between overachievement (defined as high school achievement in relation to intelligence) and catecholamine output (adrenaline and noradrenaline). The subjects were 176 children aged 13 yr. Adrenaline and noradrenaline excretions were measured in (1) a neutral situation (attending a nonexciting film), and (2) in an achievement-demanding situation. In the achievement-demanding situation, overachieving boys had much higher adrenaline excretion levels than the other boys. This difference was not found for girls. For boys, overachievement in combination with overambition (as rated by teachers) was related to a higher output of both adrenaline and noradrenaline in an achievement-demanding situation.", "contents": "Overachievement and catecholamine excretion in an achievement-demanding situation. We have studied the relationship between overachievement (defined as high school achievement in relation to intelligence) and catecholamine output (adrenaline and noradrenaline). The subjects were 176 children aged 13 yr. Adrenaline and noradrenaline excretions were measured in (1) a neutral situation (attending a nonexciting film), and (2) in an achievement-demanding situation. In the achievement-demanding situation, overachieving boys had much higher adrenaline excretion levels than the other boys. This difference was not found for girls. For boys, overachievement in combination with overambition (as rated by teachers) was related to a higher output of both adrenaline and noradrenaline in an achievement-demanding situation."}
{"id": "PMID:472085", "title": "Resentful and reflective coping with arbitrary authority and blood pressure: Detroit.", "content": "Two hypotheses about coping with an arbitrary authority, an angry boss, were tested: (1) styles of handling anger vary with social status, and (2) these styles are in turn related to blood pressure levels. Two styles of coping were tested: model R-R, Resentful vs. Reflective, and model I/O/R, Anger-In, Anger-Out, and Reflective. Handling an angry boss by Reflection is reported by more women than men, by those in middle-class areas rather than lower class, and does not vary by race. Working class report more use of Anger-Out than middle class who in turn report more use of Reflection. In general, the Anger-In response did not vary by race, sex, or area of residence (12-18%). For model R-R, Reflection of boss's anger was related to lower blood pressure when compared to Resentful responses, within sex, race, and residence groups. For model I/O/R, working-class, high stress persons who expressed Anger-Out showed the highest mean levels. Reflection is an appraisal response related to vascular and neural deceleration in stress experiments. This mode can be learned, and may aid in handling daily emotional-loaded stimuli to control blood pressure, along with learning a relaxation response.", "contents": "Resentful and reflective coping with arbitrary authority and blood pressure: Detroit. Two hypotheses about coping with an arbitrary authority, an angry boss, were tested: (1) styles of handling anger vary with social status, and (2) these styles are in turn related to blood pressure levels. Two styles of coping were tested: model R-R, Resentful vs. Reflective, and model I/O/R, Anger-In, Anger-Out, and Reflective. Handling an angry boss by Reflection is reported by more women than men, by those in middle-class areas rather than lower class, and does not vary by race. Working class report more use of Anger-Out than middle class who in turn report more use of Reflection. In general, the Anger-In response did not vary by race, sex, or area of residence (12-18%). For model R-R, Reflection of boss's anger was related to lower blood pressure when compared to Resentful responses, within sex, race, and residence groups. For model I/O/R, working-class, high stress persons who expressed Anger-Out showed the highest mean levels. Reflection is an appraisal response related to vascular and neural deceleration in stress experiments. This mode can be learned, and may aid in handling daily emotional-loaded stimuli to control blood pressure, along with learning a relaxation response."}
{"id": "PMID:472086", "title": "Impact of Event Scale: a measure of subjective stress.", "content": "Clinical, field, and experimental studies of response to potentially stressful life events give concordant findings: there is a general human tendency to undergo episodes of intrusive thinking and periods of avoidance. A scale of current subjective distress, related to a specific event, was based on a list of items composed of commonly reported experiences of intrusion and avoidance. Responses of 66 persons admitted to an outpatient clinic for the treatment of stress response syndromes indicated that the scale had a useful degree of significance and homogeneity. Empirical clusters supported the concept of subscores for intrusions and avoidance responses.", "contents": "Impact of Event Scale: a measure of subjective stress. Clinical, field, and experimental studies of response to potentially stressful life events give concordant findings: there is a general human tendency to undergo episodes of intrusive thinking and periods of avoidance. A scale of current subjective distress, related to a specific event, was based on a list of items composed of commonly reported experiences of intrusion and avoidance. Responses of 66 persons admitted to an outpatient clinic for the treatment of stress response syndromes indicated that the scale had a useful degree of significance and homogeneity. Empirical clusters supported the concept of subscores for intrusions and avoidance responses."}
{"id": "PMID:472087", "title": "Taste detection and preferences in diabetics and their relatives.", "content": "In order to determine whether a generalized defect in glucose recognition exists in diabetes, taste detection and preference were measured in adult onset diabetics (AOD), juvenile onset diabetics (JOD), and healthy first-degree relatives of diabetics (NR). Controls (C) were age and sex matched nondiabetics without first-degree diabetic relatives. The AOD and NR gorups showed significantly higher glucose thresholds than their controls. In contrast, glucose threshold in JOD was not different from C. The AOD group also demonstrated a higher sucrose threshold than C. This difference was not present for JOD or NR groups. No difference in salt detection was seen in any of the groups. Taste preference was assessed by two choice situations and ratings of test solutions of varying concentrations. No significant difference in glucose or sucrose preference were noted, but both the AOD and NR groups preferred lower salt concentrations than C. These findings indicate that thery may be a widespread impariment of cellular glucose recognition in AOD and their relatives, while JOD have a specific beta cell defect.", "contents": "Taste detection and preferences in diabetics and their relatives. In order to determine whether a generalized defect in glucose recognition exists in diabetes, taste detection and preference were measured in adult onset diabetics (AOD), juvenile onset diabetics (JOD), and healthy first-degree relatives of diabetics (NR). Controls (C) were age and sex matched nondiabetics without first-degree diabetic relatives. The AOD and NR gorups showed significantly higher glucose thresholds than their controls. In contrast, glucose threshold in JOD was not different from C. The AOD group also demonstrated a higher sucrose threshold than C. This difference was not present for JOD or NR groups. No difference in salt detection was seen in any of the groups. Taste preference was assessed by two choice situations and ratings of test solutions of varying concentrations. No significant difference in glucose or sucrose preference were noted, but both the AOD and NR groups preferred lower salt concentrations than C. These findings indicate that thery may be a widespread impariment of cellular glucose recognition in AOD and their relatives, while JOD have a specific beta cell defect."}
{"id": "PMID:472090", "title": "The epidemiology of burn injury in New York.", "content": "A population-based study of all patients hospitalized for burns in a 2-year period was conducted in up-state New York. The objective was to provide data for setting burn injury prevention priorities and for formulating treatment facility plans. The incidence rate of hospitalization for burns was 27 per 100,000 population per year. The mean estimated size of the burn wounds was 9 percent of the body surface, and fewer than 10 percent of the patients had large wounds (more than 20 percent of the body). Burn patients were admitted to hospitals of all capabilities, including 89 percent of 223 hospitals. Substantial numbers of patients with large and deep wounds were treated in hospitals with little burn treatment experience. High-risk groups were the young, blacks, and males. Burn injuries occurring at work were common. Burn injuries resulting in hospitalization occurred less frequently, and the wounds were smaller in size than would have been expected based on reports in the literature.", "contents": "The epidemiology of burn injury in New York. A population-based study of all patients hospitalized for burns in a 2-year period was conducted in up-state New York. The objective was to provide data for setting burn injury prevention priorities and for formulating treatment facility plans. The incidence rate of hospitalization for burns was 27 per 100,000 population per year. The mean estimated size of the burn wounds was 9 percent of the body surface, and fewer than 10 percent of the patients had large wounds (more than 20 percent of the body). Burn patients were admitted to hospitals of all capabilities, including 89 percent of 223 hospitals. Substantial numbers of patients with large and deep wounds were treated in hospitals with little burn treatment experience. High-risk groups were the young, blacks, and males. Burn injuries occurring at work were common. Burn injuries resulting in hospitalization occurred less frequently, and the wounds were smaller in size than would have been expected based on reports in the literature."}
{"id": "PMID:472094", "title": "Infant mortality in Newark, New Jersey. A study of sociodemographic and medical factors.", "content": "Newark, a metropolitan industrial town, experienced the highest infant mortality of any major city in the United States in the 1960s and early 1970s. Between 1970 and 1973, however, infant mortality among non-whites in this city declined strikingly. This decline could not be directly related to declines (a) in birth rates, (b) in the proportions of babies of low birth weight, (c) in the proportions of babies born to mothers in unfavorable age groups, (d) in the general fertility rates, or (e) in the illegitimacy rates. The decline may have been related (a) to the removal from childbearing cohorts of the group of females in the population--as yet undefined--whose babies would have been at high risk of infant mortality, (b) to the falling birth rate, (d) to better postnatal care--or to all of these factors. The study data suggest a multifactorial basis for the precipitous decline and also suggest that further major reductions in infant mortality among both nonwhites and whites will require better definition of the causes of low birth weight.", "contents": "Infant mortality in Newark, New Jersey. A study of sociodemographic and medical factors. Newark, a metropolitan industrial town, experienced the highest infant mortality of any major city in the United States in the 1960s and early 1970s. Between 1970 and 1973, however, infant mortality among non-whites in this city declined strikingly. This decline could not be directly related to declines (a) in birth rates, (b) in the proportions of babies of low birth weight, (c) in the proportions of babies born to mothers in unfavorable age groups, (d) in the general fertility rates, or (e) in the illegitimacy rates. The decline may have been related (a) to the removal from childbearing cohorts of the group of females in the population--as yet undefined--whose babies would have been at high risk of infant mortality, (b) to the falling birth rate, (d) to better postnatal care--or to all of these factors. The study data suggest a multifactorial basis for the precipitous decline and also suggest that further major reductions in infant mortality among both nonwhites and whites will require better definition of the causes of low birth weight."}
{"id": "PMID:472107", "title": "Effects of modulated RF energy on the EEG of mammalian brains. Effects of acute and chronic irradiations.", "content": "The effects of modulated radio frequency fields on mammalian EEGs were investigated using acute and chronic irradiations at non-thermal level. The EEG signals were computer processed to obtain power spectra. Rabbits were exposed to the field for 2 h a day for 6 weeks at 1-10 MHz (15 Hz modulation) at the level of 0.5-1 kV/M. Silver electrodes placed on the skull surface were used for recording of the EEG. Usually they were removed immediately after initial recordings of the EEG and reinserted before the final and intermediate EEG recordings. With this arrangement, modulated RF fields produced a change in EEG patterns by enhancing the low frequency components and decreasing high frequency activities. On the other hand, acute irradiations did not produce noticeable changes in the EEG at the level of 0.5-1 kV/M (1-30 MHz, 60 Hz modulation) as long as the use of intracranial electrodes was avoided.", "contents": "Effects of modulated RF energy on the EEG of mammalian brains. Effects of acute and chronic irradiations. The effects of modulated radio frequency fields on mammalian EEGs were investigated using acute and chronic irradiations at non-thermal level. The EEG signals were computer processed to obtain power spectra. Rabbits were exposed to the field for 2 h a day for 6 weeks at 1-10 MHz (15 Hz modulation) at the level of 0.5-1 kV/M. Silver electrodes placed on the skull surface were used for recording of the EEG. Usually they were removed immediately after initial recordings of the EEG and reinserted before the final and intermediate EEG recordings. With this arrangement, modulated RF fields produced a change in EEG patterns by enhancing the low frequency components and decreasing high frequency activities. On the other hand, acute irradiations did not produce noticeable changes in the EEG at the level of 0.5-1 kV/M (1-30 MHz, 60 Hz modulation) as long as the use of intracranial electrodes was avoided."}
{"id": "PMID:472108", "title": "Contrasting oxygen-effects in the inactivation of ribonuclease A by N.3, (SCN).-2 and .OH radicals.", "content": "N.3 exhibits higher efficiency than .OH in the inactivation of RNase in de-acerated (neutral) aqueous solution. In O2-saturated solution the .OH-induced inactivation is enhanced, but N.3 and (SCN).-2 become remarkably inefficient. Our results suggest that semi-oxidized tyrosine, the predominant initial defect induced by N.3 and (SCN).-2 but not by  .OH2 can be re-reduced upon reaction with O.-2 or cysteine.", "contents": "Contrasting oxygen-effects in the inactivation of ribonuclease A by N.3, (SCN).-2 and .OH radicals. N.3 exhibits higher efficiency than .OH in the inactivation of RNase in de-acerated (neutral) aqueous solution. In O2-saturated solution the .OH-induced inactivation is enhanced, but N.3 and (SCN).-2 become remarkably inefficient. Our results suggest that semi-oxidized tyrosine, the predominant initial defect induced by N.3 and (SCN).-2 but not by  .OH2 can be re-reduced upon reaction with O.-2 or cysteine."}
{"id": "PMID:472109", "title": "Effects of 226Ra and X-irradiation on the proliferative and differentiative ability of mouse hemopoietic stem cells.", "content": "The ability of hemopoietic stem cells to repopulate spleens of heavily irradiated syngeneic hosts in form of macroscopically visible clonal colonies of differentiating cells was studied in mice exposed for 32 and 4 weeks to internally deposited 226Ra (0.56 and 0.46 muCi per mouse respectively) or to 100 rad X-irradiation. Exocolonizing test and cytological techniques were used for quantitative evaluation. The size of stem cell compartment was reduced and the function of the surviving stem cells was altered by radium and X-ray irradiation. The proliferation and maintenance of hemopoietic cell populations were found to depend not only on the numbers of stem cells but also on their multiplicative and differentiative capability.", "contents": "Effects of 226Ra and X-irradiation on the proliferative and differentiative ability of mouse hemopoietic stem cells. The ability of hemopoietic stem cells to repopulate spleens of heavily irradiated syngeneic hosts in form of macroscopically visible clonal colonies of differentiating cells was studied in mice exposed for 32 and 4 weeks to internally deposited 226Ra (0.56 and 0.46 muCi per mouse respectively) or to 100 rad X-irradiation. Exocolonizing test and cytological techniques were used for quantitative evaluation. The size of stem cell compartment was reduced and the function of the surviving stem cells was altered by radium and X-ray irradiation. The proliferation and maintenance of hemopoietic cell populations were found to depend not only on the numbers of stem cells but also on their multiplicative and differentiative capability."}
{"id": "PMID:472110", "title": "Rate constants studies of the dye-sensitized photoinactivation of lysozyme.", "content": "The rate constants for the photodynamic inactivation of hen egg-white lysozyme at different temperatures were studied. Arrhenius plots of the methylene blue sensitized photo-inactivation of lysozyme gave an experimental activation energy of 7.5 kcal/mol. The rate constants for the photodynamic inactivation of lysozyme in the presence of riboflavin decreased almost linearly in the temperature range 4-38 degrees C. The photosensitized oxidation of lysozyme at -20 degrees C in freezing and non-freezing solvents was possible only in the presence of riboflavin. The effect of dye concentration on the quantum yield and rate constant for the photodynamic inactivation of lysozyme was examined. The quantum yields were lower when the concentrations of methylene blue used were low, and increased on increasing dye concentration, getting to a maximum and then declined at higher dye concentrations. It was found that in the case of riboflavin sensitized photo-inactivation of lysozyme both the rate constant and the quantum yield increased as the dye concentration increased. No maximum was observed over the range of dye concentrations studied. A new mechanism is postulated for the photodynamic action of lysozyme in the presence of riboflavin.", "contents": "Rate constants studies of the dye-sensitized photoinactivation of lysozyme. The rate constants for the photodynamic inactivation of hen egg-white lysozyme at different temperatures were studied. Arrhenius plots of the methylene blue sensitized photo-inactivation of lysozyme gave an experimental activation energy of 7.5 kcal/mol. The rate constants for the photodynamic inactivation of lysozyme in the presence of riboflavin decreased almost linearly in the temperature range 4-38 degrees C. The photosensitized oxidation of lysozyme at -20 degrees C in freezing and non-freezing solvents was possible only in the presence of riboflavin. The effect of dye concentration on the quantum yield and rate constant for the photodynamic inactivation of lysozyme was examined. The quantum yields were lower when the concentrations of methylene blue used were low, and increased on increasing dye concentration, getting to a maximum and then declined at higher dye concentrations. It was found that in the case of riboflavin sensitized photo-inactivation of lysozyme both the rate constant and the quantum yield increased as the dye concentration increased. No maximum was observed over the range of dye concentrations studied. A new mechanism is postulated for the photodynamic action of lysozyme in the presence of riboflavin."}
{"id": "PMID:472111", "title": "Changes in production, yield, and chemical composition of corn (Zea mays) after ultrasound treatments of the seeds.", "content": "In the spring of each year (1972-1974) air-dry grains of two inbred lines of Z. mays (7275-13-1 and 106) were exposed for 17 h to a low level (25 kHz) of ultrasound and subsequently planted in the field together with untreated controls and grown to maturity. In the fall of each year the ears of each group were harvested and the number of broken stalks, the yield and weight of grains and grain parts and the alcohol-soluble amino acid complement of the grain parts were determined. Broken stalks and yields were unaffected, however the embryos from the treated groups retained more moisture than their respective controls, dry weights were also significantly increased. The alcohol-soluble amino acid composition of the embryos was markedly changed, levels of proline were severely depressed in the embryos of both lines and generally increased in the endosperms. Sonication resulted in an overall decrease of up to 40% in totalled alcohol-soluble amino acids in the grains of 7275-13-1.", "contents": "Changes in production, yield, and chemical composition of corn (Zea mays) after ultrasound treatments of the seeds. In the spring of each year (1972-1974) air-dry grains of two inbred lines of Z. mays (7275-13-1 and 106) were exposed for 17 h to a low level (25 kHz) of ultrasound and subsequently planted in the field together with untreated controls and grown to maturity. In the fall of each year the ears of each group were harvested and the number of broken stalks, the yield and weight of grains and grain parts and the alcohol-soluble amino acid complement of the grain parts were determined. Broken stalks and yields were unaffected, however the embryos from the treated groups retained more moisture than their respective controls, dry weights were also significantly increased. The alcohol-soluble amino acid composition of the embryos was markedly changed, levels of proline were severely depressed in the embryos of both lines and generally increased in the endosperms. Sonication resulted in an overall decrease of up to 40% in totalled alcohol-soluble amino acids in the grains of 7275-13-1."}
{"id": "PMID:472112", "title": "Hypophysectomy-induced inhibition of augmented acetylcholine responses of the rat bowel following adrenalectomy and/or whole body irradiation.", "content": "Functional changes in the intestinal responsiveness to a fixed dose of acetylcholine were studied in muscle strips removed from young adult male rats previously exposed to whole body gamma radiation. In the irradiated rat the responsiveness to a fixed dose of acetylcholine was found to be augmented in the small intestine but not in the colon. Similar motor patterns for the small intestine were found when muscle strips from adrenalectomized rats were studied. Preradiation adrenalectomy further exaggerated the post-radiation sensitivity of the rat small intestine to acetylcholine. Hypophysectomy prior to either adrenalectomy and/or whole body radiation was associated with an absence of augmented small intestinal motor activity following administration of acetylcholine. The response of the large bowel to acetylcholine, however, was not modified by adrenalectomy and/or hypophysectomy. These observations suggest an endocrine component to the acute 3--5 day intestinal radiation syndrome.", "contents": "Hypophysectomy-induced inhibition of augmented acetylcholine responses of the rat bowel following adrenalectomy and/or whole body irradiation. Functional changes in the intestinal responsiveness to a fixed dose of acetylcholine were studied in muscle strips removed from young adult male rats previously exposed to whole body gamma radiation. In the irradiated rat the responsiveness to a fixed dose of acetylcholine was found to be augmented in the small intestine but not in the colon. Similar motor patterns for the small intestine were found when muscle strips from adrenalectomized rats were studied. Preradiation adrenalectomy further exaggerated the post-radiation sensitivity of the rat small intestine to acetylcholine. Hypophysectomy prior to either adrenalectomy and/or whole body radiation was associated with an absence of augmented small intestinal motor activity following administration of acetylcholine. The response of the large bowel to acetylcholine, however, was not modified by adrenalectomy and/or hypophysectomy. These observations suggest an endocrine component to the acute 3--5 day intestinal radiation syndrome."}
{"id": "PMID:472113", "title": "Tube growth stimulation of pine pollen by low doses of irradiation. Dose rate, reproducibility and comparison between UV-light and ionizing rays.", "content": "Stimulation of the tube growth of pine pollen by low doses of UV-light or X-rays is a reproducible process, but it depends strongly of the dose rate applied. It can be proved that the observed effect is more determined by dose rate and irradiation time than by the dose itself which can vary by nearly one order of magnitude for achieving the same increase in tube elongation. The range of absorbed energy at which the stimulation effect can be observed is rather broad and overlapping between UV and X-rays. In the average the UV-energy needed is 5 times higher than X-ray energy.", "contents": "Tube growth stimulation of pine pollen by low doses of irradiation. Dose rate, reproducibility and comparison between UV-light and ionizing rays. Stimulation of the tube growth of pine pollen by low doses of UV-light or X-rays is a reproducible process, but it depends strongly of the dose rate applied. It can be proved that the observed effect is more determined by dose rate and irradiation time than by the dose itself which can vary by nearly one order of magnitude for achieving the same increase in tube elongation. The range of absorbed energy at which the stimulation effect can be observed is rather broad and overlapping between UV and X-rays. In the average the UV-energy needed is 5 times higher than X-ray energy."}
{"id": "PMID:472114", "title": "Changes in the circular dichroic spectrum of calf thymus solubilized chromatin caused by ultraviolet irradiation.", "content": "UV irradiation of the chromatin caused an increase of the positive circular dichroic band in the vicinity of 275 nm (corresponding to DNA) and a deepening of the negative band of proteins at about 225 nm. These changes in the circular dichroic spectrum are monotonous in the range of doses studied (less than 6 X 10(4) J.m-2). The increase of the positive circular dichroic band probably reflects the occurrence of local conformational changes in DNA, which include changes in base position (tilting, distance from helix axis) in the close neighborhood of photoproducts. The presence of photoproducts in chromatin reduces changes in its circular dichroic spectra with temperature.", "contents": "Changes in the circular dichroic spectrum of calf thymus solubilized chromatin caused by ultraviolet irradiation. UV irradiation of the chromatin caused an increase of the positive circular dichroic band in the vicinity of 275 nm (corresponding to DNA) and a deepening of the negative band of proteins at about 225 nm. These changes in the circular dichroic spectrum are monotonous in the range of doses studied (less than 6 X 10(4) J.m-2). The increase of the positive circular dichroic band probably reflects the occurrence of local conformational changes in DNA, which include changes in base position (tilting, distance from helix axis) in the close neighborhood of photoproducts. The presence of photoproducts in chromatin reduces changes in its circular dichroic spectra with temperature."}
{"id": "PMID:472115", "title": "[The action of hyperthermia on DNA repair (author's transl)].", "content": "The time-course of DNA repair after gamma irradiation was measured in HeLa cells at various temperatures. Unscheduled DNA synthesis was estimated by incorporation of 3H-thymidine in presence of hydroxyurea. To detect the ligase reaction, the number of single strand breaks (SSB) was determined by centrifugation in alcaline sucrose as well as by hydroxylapatite chromatography after partial denaturation. In addition, the temperature dependence of DNA polymerase and DNase reaction in cell-free systems were measured. These data were compared with the reduction of colony-forming ability of the cells caused by gamma irradiation and following repair at various temperatures. All steps of repair proceed faster at 41--43 degrees than at 37 degrees but cells are most resistant to gamma irradiation at 37 degrees. We therefore assume that the DNA repair process at 42 degrees is faster but more error prone than at 37 degrees.", "contents": "[The action of hyperthermia on DNA repair (author's transl)]. The time-course of DNA repair after gamma irradiation was measured in HeLa cells at various temperatures. Unscheduled DNA synthesis was estimated by incorporation of 3H-thymidine in presence of hydroxyurea. To detect the ligase reaction, the number of single strand breaks (SSB) was determined by centrifugation in alcaline sucrose as well as by hydroxylapatite chromatography after partial denaturation. In addition, the temperature dependence of DNA polymerase and DNase reaction in cell-free systems were measured. These data were compared with the reduction of colony-forming ability of the cells caused by gamma irradiation and following repair at various temperatures. All steps of repair proceed faster at 41--43 degrees than at 37 degrees but cells are most resistant to gamma irradiation at 37 degrees. We therefore assume that the DNA repair process at 42 degrees is faster but more error prone than at 37 degrees."}
{"id": "PMID:472116", "title": "Occupational dose from natural radionuclides in phosphate fertilizers.", "content": "In this investigation the occupational exposure of single persons due to the gamma radiation of the natural radionuclides in rock phosphates and phosphate fertilizers and their contribution to the population dose in the FRG has been determined. The exposure rates in the working fields production, transport, loading and storage or rock phosphates and phosphate fertilizers and due to their application in agriculture have been measured by means of scintillation dose rate meters or LiF-thermoluminescence dosemeters or have been estimated from specific activities. Mean additional exposure rates of 2--26 muR/h, with local maximum values up to 190 muR/h, were observed. From these values, together with statistical data for the number of occupied persons and annual working times in the various working fields, the mean and maximum annual dose of individuals and the contribution to the mean population dose have been estimated. The results show that a maximum annual dose to individuals from 0.4 mrem/y (agriculture) up to 45 mrem/y (production plants or storehouses) can occur. The corresponding mean annual doses are 0.05--20 mrem/y. The contribution of the occupational radiation exposure due to rock phosphates and phosphate fertilizers to the mean population dose is 174 man . rem/y related to whole body. To this, fertilizer production contributes 40 man . rem/y, transport and loading 45 man . rem/y, agricultural storehouses 31 man . rem/y, and agriculture 58 man . rem/y. Altogether, this investigation shows that an occupational radiation exposure of individuals may occur which corresponds to the mean terrestrial radiation exposure in the FRG. The contribution of the occupational collective doses due to phosphates to the population dose, however, is negligibly small.", "contents": "Occupational dose from natural radionuclides in phosphate fertilizers. In this investigation the occupational exposure of single persons due to the gamma radiation of the natural radionuclides in rock phosphates and phosphate fertilizers and their contribution to the population dose in the FRG has been determined. The exposure rates in the working fields production, transport, loading and storage or rock phosphates and phosphate fertilizers and due to their application in agriculture have been measured by means of scintillation dose rate meters or LiF-thermoluminescence dosemeters or have been estimated from specific activities. Mean additional exposure rates of 2--26 muR/h, with local maximum values up to 190 muR/h, were observed. From these values, together with statistical data for the number of occupied persons and annual working times in the various working fields, the mean and maximum annual dose of individuals and the contribution to the mean population dose have been estimated. The results show that a maximum annual dose to individuals from 0.4 mrem/y (agriculture) up to 45 mrem/y (production plants or storehouses) can occur. The corresponding mean annual doses are 0.05--20 mrem/y. The contribution of the occupational radiation exposure due to rock phosphates and phosphate fertilizers to the mean population dose is 174 man . rem/y related to whole body. To this, fertilizer production contributes 40 man . rem/y, transport and loading 45 man . rem/y, agricultural storehouses 31 man . rem/y, and agriculture 58 man . rem/y. Altogether, this investigation shows that an occupational radiation exposure of individuals may occur which corresponds to the mean terrestrial radiation exposure in the FRG. The contribution of the occupational collective doses due to phosphates to the population dose, however, is negligibly small."}
{"id": "PMID:472117", "title": "Influence of caffeine on sparing effect of dose fractionation in housefly larvae.", "content": "Caffeine, given during interfraction interval, abolishes the sparing effect of dose fractionation observed for delay of pupariation in Musca domestica larvae. When given as postirradiation treatment after single exposure, caffeine increases the delay in the synergistic manner. Pretreatment of the larvae with ascorbic acid for 3 h protects from radiation-induced delay whereas pretreatment with caffeine does not have any effect. Combination of ascorbic acid and caffeine pretreatment protects the larvae only at low concentration (0.05%) and not at high concentration (0.1%).", "contents": "Influence of caffeine on sparing effect of dose fractionation in housefly larvae. Caffeine, given during interfraction interval, abolishes the sparing effect of dose fractionation observed for delay of pupariation in Musca domestica larvae. When given as postirradiation treatment after single exposure, caffeine increases the delay in the synergistic manner. Pretreatment of the larvae with ascorbic acid for 3 h protects from radiation-induced delay whereas pretreatment with caffeine does not have any effect. Combination of ascorbic acid and caffeine pretreatment protects the larvae only at low concentration (0.05%) and not at high concentration (0.1%)."}
{"id": "PMID:472118", "title": "Radiation induced chromosome aberrations and the Poisson distribution.", "content": "Data on the distribution of dicentrics and acentrics observed when human lymphocytes are cultured for 48 h after irradiation by X-rays, gamma-rays, and neutrons are presented. Analysis shows that for dicentrics, the observed distribution for X-rays, gamma-rays, and fission neutrons may be described by Poisson statistics but for higher energy neutrons overdispersion is observed. The phenomenon of overdispersion is also observed for acentrics irrespective of the radiation used. The possibility that overdispersion results from the variations of dose in sensitive sites leads to the conclusion that for dicentrics the site size is considerably larger than the 1--2 micrometer diameter derived by applying the dual action theory to the dose effect relationships. This larger site may well be the cell nucleus.", "contents": "Radiation induced chromosome aberrations and the Poisson distribution. Data on the distribution of dicentrics and acentrics observed when human lymphocytes are cultured for 48 h after irradiation by X-rays, gamma-rays, and neutrons are presented. Analysis shows that for dicentrics, the observed distribution for X-rays, gamma-rays, and fission neutrons may be described by Poisson statistics but for higher energy neutrons overdispersion is observed. The phenomenon of overdispersion is also observed for acentrics irrespective of the radiation used. The possibility that overdispersion results from the variations of dose in sensitive sites leads to the conclusion that for dicentrics the site size is considerably larger than the 1--2 micrometer diameter derived by applying the dual action theory to the dose effect relationships. This larger site may well be the cell nucleus."}
{"id": "PMID:472199", "title": "The adult male urethra: normal anatomy, pathology, and method of urethrography.", "content": "Detailed knowledge of the normal anatomy of the urethra and adjacent structures is essential to an understanding of the normal radiologic landmarks on urethrography, and is prerequisite to the radiologic interpretation of the diseased urethra. The nature of fibrosis in the urethra must also be clearly understood, since radiologic interpretation of urethral scarring influences surgical management. No longer is it sufficient for the radiologist to delineate a tight structure in the urethra and leave the operative decision solely to clinical assessment. Dynamic retrograde urethrography allows the radiologist to direct the attention of the urologist to the membranous urethra and to the possibility that transsphincter urethroplasty may be required. While voiding urethrography is an integral part of the examination and may provide additional information, voiding urethrography without dynamic retrograde urethrography without dynamic retrograde urethrography may be misleading in that soft scarring of the urethra may be missed.", "contents": "The adult male urethra: normal anatomy, pathology, and method of urethrography. Detailed knowledge of the normal anatomy of the urethra and adjacent structures is essential to an understanding of the normal radiologic landmarks on urethrography, and is prerequisite to the radiologic interpretation of the diseased urethra. The nature of fibrosis in the urethra must also be clearly understood, since radiologic interpretation of urethral scarring influences surgical management. No longer is it sufficient for the radiologist to delineate a tight structure in the urethra and leave the operative decision solely to clinical assessment. Dynamic retrograde urethrography allows the radiologist to direct the attention of the urologist to the membranous urethra and to the possibility that transsphincter urethroplasty may be required. While voiding urethrography is an integral part of the examination and may provide additional information, voiding urethrography without dynamic retrograde urethrography without dynamic retrograde urethrography may be misleading in that soft scarring of the urethra may be missed."}
{"id": "PMID:472220", "title": "Adjustable compensating filters for pediatric 72-inch spine radiography.", "content": "The difficulty in producing a uniformly penetrated radiograph of the upright spine at 72 inches is alleviated by the use of compensating filters. These filters are finely machined, adjustable and easy to position. High quality, uniform density radiographs are now easily obtained on patients of all sizes. Most importantly, patient dose has been reduced by a factor of 2.4 to 6.9, a feature that makes their use mandatory in pediatric spine radiography.", "contents": "Adjustable compensating filters for pediatric 72-inch spine radiography. The difficulty in producing a uniformly penetrated radiograph of the upright spine at 72 inches is alleviated by the use of compensating filters. These filters are finely machined, adjustable and easy to position. High quality, uniform density radiographs are now easily obtained on patients of all sizes. Most importantly, patient dose has been reduced by a factor of 2.4 to 6.9, a feature that makes their use mandatory in pediatric spine radiography."}
{"id": "PMID:472223", "title": "Radiation dose in neurological computed tomographic scanning.", "content": "Patient dose and dose distribution during neurological computed tomography examinations were determined with five different computed tomography scanners. Maximum intracranial doses ranged from 1.17 to 2.67 rads. Doses to the lens of the eye ranged from 0.23 to 2.81 rads. These levels are considered and compared with patient doses reported for other computed tomography studies and for conventional tomographic examinations. In general, patient dose during computer tomographic examinations is less than one quarter of that during conventional tomography of the head.", "contents": "Radiation dose in neurological computed tomographic scanning. Patient dose and dose distribution during neurological computed tomography examinations were determined with five different computed tomography scanners. Maximum intracranial doses ranged from 1.17 to 2.67 rads. Doses to the lens of the eye ranged from 0.23 to 2.81 rads. These levels are considered and compared with patient doses reported for other computed tomography studies and for conventional tomographic examinations. In general, patient dose during computer tomographic examinations is less than one quarter of that during conventional tomography of the head."}
{"id": "PMID:472224", "title": "Improving patient cooperation.", "content": "Patient anxiety and poor cooperation are more apt to be socio-psychological in origin rather than mental or physical. Therefore, it is important that the technology curriculum provide sufficient emphasis on behavioral psychology, motivation and control techniques, and communication skills. Studies show that if the patient and his family are adequately informed of what to expect when they go to the radiology department, through interviews, videotapes, etc., their anxieties decrease. Use of anxiety-reducing techniques can decrease uncooperative behavior, examination time, and misunderstandings that cause a poor professional image.", "contents": "Improving patient cooperation. Patient anxiety and poor cooperation are more apt to be socio-psychological in origin rather than mental or physical. Therefore, it is important that the technology curriculum provide sufficient emphasis on behavioral psychology, motivation and control techniques, and communication skills. Studies show that if the patient and his family are adequately informed of what to expect when they go to the radiology department, through interviews, videotapes, etc., their anxieties decrease. Use of anxiety-reducing techniques can decrease uncooperative behavior, examination time, and misunderstandings that cause a poor professional image."}
{"id": "PMID:472230", "title": "Etiologic diagnosis of focal pulmonary infection in immunocompromised patients by fluoroscopically guided percutaneous needle aspiration.", "content": "Fluoroscopically guided percutaneous needle aspiration of focal pulmonary lesions was performed in 108 presumed infectious episodes in 82 immunocompromised patients in whom prior diagnostic studies, including transtracheal aspiration, were negative to inconclusive. Two-thirds of the lesions were 4 cm or smaller. Single (61/79) or multiple (18/79) organisms were recovered, for a diagnostic yield of 73% (79/108). Complications were pneumothorax (26%), half of which required tube drainage, and limited hemoptysis (3%). This technique can be quickly performed and frequently repeated with existing personnel and equipment in institutions currently employing it for suspected pulmonary neoplasm, and is a productive study in the evaluation of \"opportunistic pneumonia\".", "contents": "Etiologic diagnosis of focal pulmonary infection in immunocompromised patients by fluoroscopically guided percutaneous needle aspiration. Fluoroscopically guided percutaneous needle aspiration of focal pulmonary lesions was performed in 108 presumed infectious episodes in 82 immunocompromised patients in whom prior diagnostic studies, including transtracheal aspiration, were negative to inconclusive. Two-thirds of the lesions were 4 cm or smaller. Single (61/79) or multiple (18/79) organisms were recovered, for a diagnostic yield of 73% (79/108). Complications were pneumothorax (26%), half of which required tube drainage, and limited hemoptysis (3%). This technique can be quickly performed and frequently repeated with existing personnel and equipment in institutions currently employing it for suspected pulmonary neoplasm, and is a productive study in the evaluation of \"opportunistic pneumonia\"."}
{"id": "PMID:472231", "title": "Digital radiology in trauma using small-dose exposure.", "content": "The microdose radiology system produces a large-field examination within thirty seconds on a video monitor. This video image can be manipulated in the same manner as a computed tomography (CT) scan. The system was installed at the Maryland Institute for Emergency Services (MIEMS) to examine severely injured patients. No major abnormalities were overlooked during the examination of these patients. The microdose system is useful in providing extensive radiologic information immediately applicable to the care of massively traumatized patients.", "contents": "Digital radiology in trauma using small-dose exposure. The microdose radiology system produces a large-field examination within thirty seconds on a video monitor. This video image can be manipulated in the same manner as a computed tomography (CT) scan. The system was installed at the Maryland Institute for Emergency Services (MIEMS) to examine severely injured patients. No major abnormalities were overlooked during the examination of these patients. The microdose system is useful in providing extensive radiologic information immediately applicable to the care of massively traumatized patients."}
{"id": "PMID:472232", "title": "Gastric varices secondary to splenic vein occlusion: radiographic diagnosis and clinical significance.", "content": "The radiographic appearance and clinical significance of gastric varices in the absence of esophageal varices and secondary to splenic vein occlusion were studied. Eighteen patients were evaluated through medical records, angiography, and barium studies of the stomach and esophagus. The presence of splenic vein occlusion was determined by arteriography in 18 patients and its etiology confirmed by surgery in 17 patients. This condition should be suspected in patients with chronic abdominal pain, weight loss, and iron deficiency anemia who show fundal polypoid filling defects or prominent gastric folds on an upper GI series.", "contents": "Gastric varices secondary to splenic vein occlusion: radiographic diagnosis and clinical significance. The radiographic appearance and clinical significance of gastric varices in the absence of esophageal varices and secondary to splenic vein occlusion were studied. Eighteen patients were evaluated through medical records, angiography, and barium studies of the stomach and esophagus. The presence of splenic vein occlusion was determined by arteriography in 18 patients and its etiology confirmed by surgery in 17 patients. This condition should be suspected in patients with chronic abdominal pain, weight loss, and iron deficiency anemia who show fundal polypoid filling defects or prominent gastric folds on an upper GI series."}
{"id": "PMID:472233", "title": "Therapeutic embolization of facial arteriovenous fistulae.", "content": "Two cases of percutaneous transfemoral embolization of facial hemangiomas and arteriovenous (AV) malformations are reported. In both cases, Ivalon shavings were injected through a percutaneously placed catheter to permanently occlude peripheral feeders of the AV malformation. In one case, because of the size of the large venous sacs associated with the malformation, a steel coil was introduced to form a baffle, over which several additional strands of surgical silk were introduced to obliterate these sacs. These cases emphasize the need for embolization of small feeding arteries to prevent recurrence by collateral channels. To achieve this, specific-sized emboli are necessary; Ivalon is a suitable embolic material.", "contents": "Therapeutic embolization of facial arteriovenous fistulae. Two cases of percutaneous transfemoral embolization of facial hemangiomas and arteriovenous (AV) malformations are reported. In both cases, Ivalon shavings were injected through a percutaneously placed catheter to permanently occlude peripheral feeders of the AV malformation. In one case, because of the size of the large venous sacs associated with the malformation, a steel coil was introduced to form a baffle, over which several additional strands of surgical silk were introduced to obliterate these sacs. These cases emphasize the need for embolization of small feeding arteries to prevent recurrence by collateral channels. To achieve this, specific-sized emboli are necessary; Ivalon is a suitable embolic material."}
{"id": "PMID:472234", "title": "Angiography of intussusception of the small bowel.", "content": "Angiography demonstrated intussusception in a patient with recurrent unexplained gastrointestinal hemorrhage after outside barium studies had been interpreted as normal. Magnification arteriography provided an unusually clear depiction of the anatomic derangement of intussusception.", "contents": "Angiography of intussusception of the small bowel. Angiography demonstrated intussusception in a patient with recurrent unexplained gastrointestinal hemorrhage after outside barium studies had been interpreted as normal. Magnification arteriography provided an unusually clear depiction of the anatomic derangement of intussusception."}
{"id": "PMID:472235", "title": "The patient's role in assessing the value of diagnostic tests.", "content": "Diagnostic tests are usually evaluated for their informational content and effect on health outcomes. Yet patient attitudes toward taking risks and the morbid sequelae associated with either the diagnostic test or its consequences are seldom considered. The authors present a prototypical model which incorporates patient attitudes into the evaluative process. Based on traditional indices of test efficacy, preoperative searches for occult metastases in patients with presumably operable bronchogenic carcinoma should never be performed. However, if patient attitudes toward perioperative death are considered, many should have preoperative staging tests. The exact percentage of patients benefiting from testing varies with test sensitivity and specificity. This integrative approach is appropriate for oncologic patients who frequently undergo treatment.", "contents": "The patient's role in assessing the value of diagnostic tests. Diagnostic tests are usually evaluated for their informational content and effect on health outcomes. Yet patient attitudes toward taking risks and the morbid sequelae associated with either the diagnostic test or its consequences are seldom considered. The authors present a prototypical model which incorporates patient attitudes into the evaluative process. Based on traditional indices of test efficacy, preoperative searches for occult metastases in patients with presumably operable bronchogenic carcinoma should never be performed. However, if patient attitudes toward perioperative death are considered, many should have preoperative staging tests. The exact percentage of patients benefiting from testing varies with test sensitivity and specificity. This integrative approach is appropriate for oncologic patients who frequently undergo treatment."}
{"id": "PMID:472236", "title": "Familial congenital bowing with short bones.", "content": "The case reports of 2 brothers with congenital bowing and short broad bones are discussed. In this apparently new familial bowing syndrome, the rhizometic portions of the limbs are more severely involved, particularly the femora. In early infancy the metaphyses are moderately flared and irregular, but these improve dramatically during childhood. Clinical abnormalities are limited primarily to the thorax. Prognosis appears good except for the persisting disproportionately short stature. These 2 patients may resemble one or both patients previously reported by Khajavi et al. under the label of short-limbed campomelic syndrome, normocephalic type (1).", "contents": "Familial congenital bowing with short bones. The case reports of 2 brothers with congenital bowing and short broad bones are discussed. In this apparently new familial bowing syndrome, the rhizometic portions of the limbs are more severely involved, particularly the femora. In early infancy the metaphyses are moderately flared and irregular, but these improve dramatically during childhood. Clinical abnormalities are limited primarily to the thorax. Prognosis appears good except for the persisting disproportionately short stature. These 2 patients may resemble one or both patients previously reported by Khajavi et al. under the label of short-limbed campomelic syndrome, normocephalic type (1)."}
{"id": "PMID:472237", "title": "Aortic aneurysms in children.", "content": "Three children with aneurysms of the thoracic aorta were studied. The clinical presentation, radiographic findings, and etiologic factors are discussed. These lesions are associated with a poor prognosis and only one patient survived. The radiographic appearance of an enlarging mediastinal mass close to the aorta should suggest an aneurysm of the thoracic aorta.", "contents": "Aortic aneurysms in children. Three children with aneurysms of the thoracic aorta were studied. The clinical presentation, radiographic findings, and etiologic factors are discussed. These lesions are associated with a poor prognosis and only one patient survived. The radiographic appearance of an enlarging mediastinal mass close to the aorta should suggest an aneurysm of the thoracic aorta."}
{"id": "PMID:472239", "title": "Catheter and material selection for transarterial embolization: technical considerations. I. Catheters.", "content": "In this first part of the report, the authors discuss the selection of catheterization techniques and delivery systems. These include various introducer sheaths, tapered and non-tapered conventional catheters, and double-lumen, single-lumen, flow-guided single-lumen, and detachable balloon catheters. The selection is discussed with regard to the specifics of the lesion, such as location of the lesion and number and size of feeding arteries. The authors' experience with experimental embolizations in dogs and the clinical material in 34 patients in whom 61 embolization procedures were performed are presented.", "contents": "Catheter and material selection for transarterial embolization: technical considerations. I. Catheters. In this first part of the report, the authors discuss the selection of catheterization techniques and delivery systems. These include various introducer sheaths, tapered and non-tapered conventional catheters, and double-lumen, single-lumen, flow-guided single-lumen, and detachable balloon catheters. The selection is discussed with regard to the specifics of the lesion, such as location of the lesion and number and size of feeding arteries. The authors' experience with experimental embolizations in dogs and the clinical material in 34 patients in whom 61 embolization procedures were performed are presented."}
{"id": "PMID:472240", "title": "Catheter and material selection for transarterial embolization: technical considerations. II. Materials.", "content": "In this second part of the report, the authors discuss the advantages and disadvantages of several embolization agents. These include Gelfoam, silicone spheres, polyvinyl alcohol foam (PVA), isobutyl-2-cyanoacrylate (IBCA), silicone fluid mixtures, and tantalum powder. The techniques employed and conditions under which these materials should be used are discussed.", "contents": "Catheter and material selection for transarterial embolization: technical considerations. II. Materials. In this second part of the report, the authors discuss the advantages and disadvantages of several embolization agents. These include Gelfoam, silicone spheres, polyvinyl alcohol foam (PVA), isobutyl-2-cyanoacrylate (IBCA), silicone fluid mixtures, and tantalum powder. The techniques employed and conditions under which these materials should be used are discussed."}
{"id": "PMID:472241", "title": "Computed tomography of paranasal sinus tumors.", "content": "Thirty-two patients with histologically proved malignant disease involving the paranasal sinuses were studied by CT. The radiological features of tumor were sinus opacification, a soft-tissue mass, bone erosion and/or displacement, sclerosis, and new-bone formation. Measurements of tissue densities were not helpful in distinguishing tumor from benign disease. Significantly greater tumor extent was demonstrated by CT than by conventional methods in 15 patients; the additional tumor most commonly involved the pterygoid region or orbit. The clinical importance of various directions of tumor spread is emphasized.", "contents": "Computed tomography of paranasal sinus tumors. Thirty-two patients with histologically proved malignant disease involving the paranasal sinuses were studied by CT. The radiological features of tumor were sinus opacification, a soft-tissue mass, bone erosion and/or displacement, sclerosis, and new-bone formation. Measurements of tissue densities were not helpful in distinguishing tumor from benign disease. Significantly greater tumor extent was demonstrated by CT than by conventional methods in 15 patients; the additional tumor most commonly involved the pterygoid region or orbit. The clinical importance of various directions of tumor spread is emphasized."}
{"id": "PMID:472242", "title": "Specific value of computed tomographic breast scanner (CT/M) in diagnosis of breast diseases.", "content": "CT/M study of the breast yields specific information about lesions and may provide the diagnosis when mammography fails to demonstrate the lesion or is unable to display the information necessary to make a definitive diagnosis. By using contrast medium to increase attenuation, very small carcinomas may be identified, even in dysplastic breasts.", "contents": "Specific value of computed tomographic breast scanner (CT/M) in diagnosis of breast diseases. CT/M study of the breast yields specific information about lesions and may provide the diagnosis when mammography fails to demonstrate the lesion or is unable to display the information necessary to make a definitive diagnosis. By using contrast medium to increase attenuation, very small carcinomas may be identified, even in dysplastic breasts."}
{"id": "PMID:472243", "title": "Computed vs. conventional tomography in evaluation of primary and secondary pulmonary neoplasms.", "content": "One hundred patients, ultimately proved to have chest malignancies, were evaluated prospectively with conventional chest tomography and computed tomography. In 58 patients with primary malignancies, conventional tomograms were more useful in evaluation of the hilus than CT scans. The mediastinum was better assessed by CT. Thus, evaluation of the presence of neoplasia is better accomplished by conventional examination, while extent of disease is best assessed by CT. Thoracotomy for curative resection was not attempted (in the latter cases of this series) based on CT findings of mediastinal involvement. In 42 patients with metastases to the chest, CT scans of the lung parenchyma were more sensitive than whole lung tomography but had little additional impact on patient treatment. Nevertheless, in 18 patients the results of CT or whole lung tomography directly affected patient therapy.", "contents": "Computed vs. conventional tomography in evaluation of primary and secondary pulmonary neoplasms. One hundred patients, ultimately proved to have chest malignancies, were evaluated prospectively with conventional chest tomography and computed tomography. In 58 patients with primary malignancies, conventional tomograms were more useful in evaluation of the hilus than CT scans. The mediastinum was better assessed by CT. Thus, evaluation of the presence of neoplasia is better accomplished by conventional examination, while extent of disease is best assessed by CT. Thoracotomy for curative resection was not attempted (in the latter cases of this series) based on CT findings of mediastinal involvement. In 42 patients with metastases to the chest, CT scans of the lung parenchyma were more sensitive than whole lung tomography but had little additional impact on patient treatment. Nevertheless, in 18 patients the results of CT or whole lung tomography directly affected patient therapy."}
{"id": "PMID:472244", "title": "Diagnosis of Caroli disease by computed tomography: report of two cases.", "content": "Two patients are discussed in whom Caroli diseases was diagnosed with the aid of computed tomography (CT). One patient had the pure form of the disease, characterized primarily by saccular dilatation of the intrahepatic bile ducts and clinically manifesting as recurrent cholangitis. The second patient had a form ccharacterized primarily by hepatic fibrosis and clinically manifesting as portal hypertension. These are the first patients studied with CT to be reported to the authors' knowledge; CT is recommended as the optimal noninvasive method to evaluate such patients.", "contents": "Diagnosis of Caroli disease by computed tomography: report of two cases. Two patients are discussed in whom Caroli diseases was diagnosed with the aid of computed tomography (CT). One patient had the pure form of the disease, characterized primarily by saccular dilatation of the intrahepatic bile ducts and clinically manifesting as recurrent cholangitis. The second patient had a form ccharacterized primarily by hepatic fibrosis and clinically manifesting as portal hypertension. These are the first patients studied with CT to be reported to the authors' knowledge; CT is recommended as the optimal noninvasive method to evaluate such patients."}
{"id": "PMID:472245", "title": "Computed tomographic demonstration of intraarterial air following hepatic artery ligation.", "content": "A branching pattern of intrahepatic air is usually attributed to air within the portal venous or biliary system. A case of such a pattern is reported in a patient with hepatic adenoma who had recently undergone hepatic artery ligation. Possible mechanisms for this phenomenon are discussed.", "contents": "Computed tomographic demonstration of intraarterial air following hepatic artery ligation. A branching pattern of intrahepatic air is usually attributed to air within the portal venous or biliary system. A case of such a pattern is reported in a patient with hepatic adenoma who had recently undergone hepatic artery ligation. Possible mechanisms for this phenomenon are discussed."}
{"id": "PMID:472246", "title": "Ultrasound evaluation of the posterior pelvic compartment.", "content": "The posterior pelvic compartment, defined as consisting primarily of the rectosigmoid colon and peritoneal-lined cul-de-sac, was evaluated in 104 patients. Tumors and pseudotumors (fluid- and feces-filled loops of bowel) were ultrasonographically analyzed according to: (a) morphology and echo characteristics, and (b) location within either the cul-de-sac or rectosigmoid colon. On routine examination, a striking similarity between pathological processes and pseudotumors was demonstrated; these were diagnosed correctly only by the use of a technique in which the rectosigmoid colon is filled with water. Combined distension of the urinary bladder and rectosigmoid colon also enabled localization and differentiation of abnormalities within the cul-de-sac.", "contents": "Ultrasound evaluation of the posterior pelvic compartment. The posterior pelvic compartment, defined as consisting primarily of the rectosigmoid colon and peritoneal-lined cul-de-sac, was evaluated in 104 patients. Tumors and pseudotumors (fluid- and feces-filled loops of bowel) were ultrasonographically analyzed according to: (a) morphology and echo characteristics, and (b) location within either the cul-de-sac or rectosigmoid colon. On routine examination, a striking similarity between pathological processes and pseudotumors was demonstrated; these were diagnosed correctly only by the use of a technique in which the rectosigmoid colon is filled with water. Combined distension of the urinary bladder and rectosigmoid colon also enabled localization and differentiation of abnormalities within the cul-de-sac."}
{"id": "PMID:472247", "title": "The sonographic appearance of acute pyelonephritis.", "content": "Acute pyelonephritis and acute ureteral obstruction often present with similar clinical and urographic findings. Ultrasound, however, can easily detect the presence of obstruction as well as demonstrate characteristic findings suggestive of acute pyelonephritis, and thus allows differentiation. In two patients with acute pyelonephritis, the ultrasonic findings consisted of a large swollen kidney with an increased anechoic corticomedullary area, with multiple scattered low-level echoes. Each of the two cases is discussed in detail.", "contents": "The sonographic appearance of acute pyelonephritis. Acute pyelonephritis and acute ureteral obstruction often present with similar clinical and urographic findings. Ultrasound, however, can easily detect the presence of obstruction as well as demonstrate characteristic findings suggestive of acute pyelonephritis, and thus allows differentiation. In two patients with acute pyelonephritis, the ultrasonic findings consisted of a large swollen kidney with an increased anechoic corticomedullary area, with multiple scattered low-level echoes. Each of the two cases is discussed in detail."}
{"id": "PMID:472248", "title": "Reliability of doppler scanning of the carotid bifurcation: angiographic correlation.", "content": "The results of continuous wave Doppler scanning of 96 common carotid arteries and their immediate branches were compared with those of selective angiography. The overall accuracy of the Doppler technique for the assessment of the common, internal and external carotid arteries was 98%, 81% and 86%, respectively with a sensitivity of 60%, 70% and 54%, and a specificity of 100%, 93%, and 96%. When stenoses of 50% were considered hemodynamically insignificant, the overall accuracy of the assessment of the common, internal and external carotid arteries rose to 100%, 93% and 93%, but the sensitivity was only 100%, 83% and 53%, whereas the specificity was 100%, 96% and 96%. This modality did not prove useful in the detection of intimal ulcers and plaques nor could the degree of stenosis be accurately graded. The above data indicate that contrast angiography remains imperative prior to surgery.", "contents": "Reliability of doppler scanning of the carotid bifurcation: angiographic correlation. The results of continuous wave Doppler scanning of 96 common carotid arteries and their immediate branches were compared with those of selective angiography. The overall accuracy of the Doppler technique for the assessment of the common, internal and external carotid arteries was 98%, 81% and 86%, respectively with a sensitivity of 60%, 70% and 54%, and a specificity of 100%, 93%, and 96%. When stenoses of 50% were considered hemodynamically insignificant, the overall accuracy of the assessment of the common, internal and external carotid arteries rose to 100%, 93% and 93%, but the sensitivity was only 100%, 83% and 53%, whereas the specificity was 100%, 96% and 96%. This modality did not prove useful in the detection of intimal ulcers and plaques nor could the degree of stenosis be accurately graded. The above data indicate that contrast angiography remains imperative prior to surgery."}
{"id": "PMID:472249", "title": "Inability to show clot: one limitation of ultrasonography of the abdominal aorta.", "content": "Ultrasonography is accurate for diagnosis of abdominal aneurysms and clots within them. However, due to technical factors, occlusive clots within the aorta can be missed even at high gain. The authors report three cases in which total occlusion of the abdominal aorta was misdiagnosed as a free lumen occupied by a small clot. An explanation for the shortcomings of ultrasound is offered.", "contents": "Inability to show clot: one limitation of ultrasonography of the abdominal aorta. Ultrasonography is accurate for diagnosis of abdominal aneurysms and clots within them. However, due to technical factors, occlusive clots within the aorta can be missed even at high gain. The authors report three cases in which total occlusion of the abdominal aorta was misdiagnosed as a free lumen occupied by a small clot. An explanation for the shortcomings of ultrasound is offered."}
{"id": "PMID:472250", "title": "Exercise radionuclide ventriculography: practical considerations and sensitivity of coronary artery disease detection.", "content": "Eighty-nine patients were evaluated for coronary artery disease (CAD) with exercise radionuclide ventriculography (ERV) and contrast coronary angiography. In 70 patients with documented lesions the ERV was abnormal in 65 for a sensitivity of 93%. In patients with normal coronary arteries, the ERV was abnormal in none for a specificity of 100%. Sensitivity of ERV for detecting CAD was affected by the level of exercise achieved. In patients with documented CAD who achieved adequate exercise (i.e., pressure rate product (PRP) greater than 250 or the development of angina or ST segment depression during exercise), the sensitivity was 98% (56 of 57 patients). In those with documented CAD who failed to achieve adequate exercise, the sensitivity was 69% (9 of 13 patients).", "contents": "Exercise radionuclide ventriculography: practical considerations and sensitivity of coronary artery disease detection. Eighty-nine patients were evaluated for coronary artery disease (CAD) with exercise radionuclide ventriculography (ERV) and contrast coronary angiography. In 70 patients with documented lesions the ERV was abnormal in 65 for a sensitivity of 93%. In patients with normal coronary arteries, the ERV was abnormal in none for a specificity of 100%. Sensitivity of ERV for detecting CAD was affected by the level of exercise achieved. In patients with documented CAD who achieved adequate exercise (i.e., pressure rate product (PRP) greater than 250 or the development of angina or ST segment depression during exercise), the sensitivity was 98% (56 of 57 patients). In those with documented CAD who failed to achieve adequate exercise, the sensitivity was 69% (9 of 13 patients)."}
{"id": "PMID:472251", "title": "Exercise radionuclide ejection fraction: correlation with exercise contrast ventriculography.", "content": "Thirty-one patients with known or suspected coronary artery disease or aortic valvular disease were studied at rest and during supine bicycle exercise with radionuclide and contrast left ventriculography. The radionuclide ejection fractions calculated independently by three observers correlated well at rest (r = 0.96) and with exercise (r = 0.94). The calculated values also correlated well with those obtained for contrast ventriculography using the area-length method Dodge (r = 0.89 at rest and r = 0.90 with exercise). The results suggest that further clinical applications of exercise radionuclide ventriculography are justified.", "contents": "Exercise radionuclide ejection fraction: correlation with exercise contrast ventriculography. Thirty-one patients with known or suspected coronary artery disease or aortic valvular disease were studied at rest and during supine bicycle exercise with radionuclide and contrast left ventriculography. The radionuclide ejection fractions calculated independently by three observers correlated well at rest (r = 0.96) and with exercise (r = 0.94). The calculated values also correlated well with those obtained for contrast ventriculography using the area-length method Dodge (r = 0.89 at rest and r = 0.90 with exercise). The results suggest that further clinical applications of exercise radionuclide ventriculography are justified."}
{"id": "PMID:472252", "title": "Computed tomography of the heart using thallium-201 in children.", "content": "Thallium-201 emission computed tomography (ECT) was performed in 3 pediatric patients in whom conventional scintigraphy was normal but there was a strong clinical suspicion of myocardial disease. Abnormalities in the distribution of myocardial perfusion appeared sharply. Single photon ECT provides a three dimensional reconstruction which results in greater enhancement since activity in overlying structures does not interfere. Its widespread use is limited only by the cost of the imaging device.", "contents": "Computed tomography of the heart using thallium-201 in children. Thallium-201 emission computed tomography (ECT) was performed in 3 pediatric patients in whom conventional scintigraphy was normal but there was a strong clinical suspicion of myocardial disease. Abnormalities in the distribution of myocardial perfusion appeared sharply. Single photon ECT provides a three dimensional reconstruction which results in greater enhancement since activity in overlying structures does not interfere. Its widespread use is limited only by the cost of the imaging device."}
{"id": "PMID:472253", "title": "Pancoast tumor: the value of high dose radiation therapy.", "content": "Twenty-six patients with superior pulmonary sulcus (Pancoast) tumors were evaluated. Twelve patients were treated with high dose radiotherapy with NSD over 1 900 rets and 10 with NSD less than 1 900 rets. Four patients were treated with combined radiation and surgery. High dose radiotherapy resulted in relief of pain in all patients but apparently did not change survival. Low dose radiotherapy offered relief of pain to only half the patients. Distant metastasis was noted in 30% of patients (15% to brain, 3.8% to liver), rib destruction in 65% and supraclavicular nodes in 19%. Only 4 patients went to surgery and 2 died during operation. We thus conclude that Pancoast tumor is essentially an inoperable disease.", "contents": "Pancoast tumor: the value of high dose radiation therapy. Twenty-six patients with superior pulmonary sulcus (Pancoast) tumors were evaluated. Twelve patients were treated with high dose radiotherapy with NSD over 1 900 rets and 10 with NSD less than 1 900 rets. Four patients were treated with combined radiation and surgery. High dose radiotherapy resulted in relief of pain in all patients but apparently did not change survival. Low dose radiotherapy offered relief of pain to only half the patients. Distant metastasis was noted in 30% of patients (15% to brain, 3.8% to liver), rib destruction in 65% and supraclavicular nodes in 19%. Only 4 patients went to surgery and 2 died during operation. We thus conclude that Pancoast tumor is essentially an inoperable disease."}
{"id": "PMID:472254", "title": "A simulation study of aliasing in computed tomography.", "content": "Aliasing in CT images is the result of discrete sampling in the data-taking process. Artifacts associated with aliasing, while object-dependent, also depend on fundamental parameters such as beam width and the number of rays and views. This dependence has been studied in simulation with a phantom designed to represent a transaxial section through the lower head. Although the simulation program assumes a stationary detector system, the results do not depend strongly on geometry. Aliasing has also been compared with other sources of artifact which are often more significant, such as beam hardening, machine alignment and noise.", "contents": "A simulation study of aliasing in computed tomography. Aliasing in CT images is the result of discrete sampling in the data-taking process. Artifacts associated with aliasing, while object-dependent, also depend on fundamental parameters such as beam width and the number of rays and views. This dependence has been studied in simulation with a phantom designed to represent a transaxial section through the lower head. Although the simulation program assumes a stationary detector system, the results do not depend strongly on geometry. Aliasing has also been compared with other sources of artifact which are often more significant, such as beam hardening, machine alignment and noise."}
{"id": "PMID:472255", "title": "Dose tables for 125I seed implants.", "content": "Dose tables are given for 125I implants, as well as detailed information about the dose distribution which is calculated for different square planar and cubic volume implants using recently published depth dose data. Minimum peripheral and maximum central doses are evaluated for different areas and volumes for seeds with 1 mCi (37 MBq) equivalent activy and spaced 1 cm apart. The dose delivered at any specific point in a permanent implant is the dose rate at that point multiplied by the mean life (2,080 hrs) and the activity per seed (1 mCi). The effect of spacing is studied and a spacing rule is suggested.", "contents": "Dose tables for 125I seed implants. Dose tables are given for 125I implants, as well as detailed information about the dose distribution which is calculated for different square planar and cubic volume implants using recently published depth dose data. Minimum peripheral and maximum central doses are evaluated for different areas and volumes for seeds with 1 mCi (37 MBq) equivalent activy and spaced 1 cm apart. The dose delivered at any specific point in a permanent implant is the dose rate at that point multiplied by the mean life (2,080 hrs) and the activity per seed (1 mCi). The effect of spacing is studied and a spacing rule is suggested."}
{"id": "PMID:472256", "title": "Synergistic biological effects of ultrasound and ionizing radiations evaluated in vitro.", "content": "The effects of diagnostic ultrasound on growing asynchronous cultures of Chinese hamster ovarian cells were evaluated, with particular attention to possible synergism with ionizing radiations. Measurements of cellular growth rate/survival and changes in morphology indicated that synergistic biological effects were absent.", "contents": "Synergistic biological effects of ultrasound and ionizing radiations evaluated in vitro. The effects of diagnostic ultrasound on growing asynchronous cultures of Chinese hamster ovarian cells were evaluated, with particular attention to possible synergism with ionizing radiations. Measurements of cellular growth rate/survival and changes in morphology indicated that synergistic biological effects were absent."}
{"id": "PMID:472257", "title": "The toposcopic catheter: a design for maneuvering through tortuous vessels.", "content": "The toposcopic catheter is an everting tube that advances within a vessel without moving over the endothelium. It easily passed through simulated tortuous vessels which other catheters could not negotiate and was also successful in the external carotid, vertebral, hepatic, renal and coronary arteries in dogs. The catheter advances to its full length (30 cm) or until wedged in small peripheral arteries.", "contents": "The toposcopic catheter: a design for maneuvering through tortuous vessels. The toposcopic catheter is an everting tube that advances within a vessel without moving over the endothelium. It easily passed through simulated tortuous vessels which other catheters could not negotiate and was also successful in the external carotid, vertebral, hepatic, renal and coronary arteries in dogs. The catheter advances to its full length (30 cm) or until wedged in small peripheral arteries."}
{"id": "PMID:472258", "title": "A hybrid computerized fluoroscopy technique for noninvasive cardiovascular imaging.", "content": "The excellent linearity of digital image storage and retrieval permits hybrid analog-digital subtraction to extend the spatial resolution of two previously developed algorithms which employed entirely digital apparatus. A low resolution, time-integrated preinjection digital mask image is reconverted to analog form and subtracted from live analog video images of iodine administered by peripheral intravenous injection to produce a high resolution display of the cardiovascular system with contrast ten times greater than conventional fluoroscopy. Preliminary studies in dogs are compared with images obtained with our digital subtraction algorithms.", "contents": "A hybrid computerized fluoroscopy technique for noninvasive cardiovascular imaging. The excellent linearity of digital image storage and retrieval permits hybrid analog-digital subtraction to extend the spatial resolution of two previously developed algorithms which employed entirely digital apparatus. A low resolution, time-integrated preinjection digital mask image is reconverted to analog form and subtracted from live analog video images of iodine administered by peripheral intravenous injection to produce a high resolution display of the cardiovascular system with contrast ten times greater than conventional fluoroscopy. Preliminary studies in dogs are compared with images obtained with our digital subtraction algorithms."}
{"id": "PMID:472261", "title": "A mechanical aid for the treatment of carcinoma of the breast.", "content": "A relatively inexpensive mechanical aid is described which, in primary or adjuvant irradiation for breast carcinoma, allows easy and stable reproducibility of the abducted arm position, unobstructed access for lateral tangential portals, and leveling of the anterior chest for regional nodal irradiation. The device may be constructed de novo or from a commonly available treatment-table accessory.", "contents": "A mechanical aid for the treatment of carcinoma of the breast. A relatively inexpensive mechanical aid is described which, in primary or adjuvant irradiation for breast carcinoma, allows easy and stable reproducibility of the abducted arm position, unobstructed access for lateral tangential portals, and leveling of the anterior chest for regional nodal irradiation. The device may be constructed de novo or from a commonly available treatment-table accessory."}
{"id": "PMID:472263", "title": "Radiation exposure to medical personnel during iodine-125 seed implantation of the prostate.", "content": "Radiation exposure to operating room personnel was measured by thermoluminescent dosimeters and an ionization chamber during a simulated iodine-125 seed implantation of the prostate gland. The exposure rates at points around the implanted phantom were measured and compared with the calculated values. The data suggested that even with a schedule of 25 prostate implants per year, exposure levels would not exceed 5% of the recommended maximum limits for occupationally exposed personnel.", "contents": "Radiation exposure to medical personnel during iodine-125 seed implantation of the prostate. Radiation exposure to operating room personnel was measured by thermoluminescent dosimeters and an ionization chamber during a simulated iodine-125 seed implantation of the prostate gland. The exposure rates at points around the implanted phantom were measured and compared with the calculated values. The data suggested that even with a schedule of 25 prostate implants per year, exposure levels would not exceed 5% of the recommended maximum limits for occupationally exposed personnel."}
{"id": "PMID:472265", "title": "Abdominal compression: a new technique for improved computed tomographic images.", "content": "A technique utilizing abdominal compression during CT scanning with resultant improvement in image quality is described. Advantages included decrease or elimination of streak artifacts; differentiation of nonopacified bowel from other soft tissue densities; and better delineation of tissue planes.", "contents": "Abdominal compression: a new technique for improved computed tomographic images. A technique utilizing abdominal compression during CT scanning with resultant improvement in image quality is described. Advantages included decrease or elimination of streak artifacts; differentiation of nonopacified bowel from other soft tissue densities; and better delineation of tissue planes."}
{"id": "PMID:472267", "title": "Single mini-catheter technique for abdominal aortography and selective injection.", "content": "A single mini-catheter technique for abdominal aortography and selective injection using 4.1 and 5 French polyethylene catheters in the Shepherd's Crook configuration is described. Studies made in 365 patients were of high quality and without complications. This technique is easily learned, less time consuming, and safer than previous pig-tailed mini-catheter and larger catheter techniques.", "contents": "Single mini-catheter technique for abdominal aortography and selective injection. A single mini-catheter technique for abdominal aortography and selective injection using 4.1 and 5 French polyethylene catheters in the Shepherd's Crook configuration is described. Studies made in 365 patients were of high quality and without complications. This technique is easily learned, less time consuming, and safer than previous pig-tailed mini-catheter and larger catheter techniques."}
{"id": "PMID:472268", "title": "Symmetrical anteroposterior projections of the sternoclavicular joints with motion studies.", "content": "A radiographic technique is described for the symmetrical visualization of the sternoclavicular joints in the anteroposterior projection. A short focal-spot patient distance is used with an ultra-fine focal-spot tube, so that differential magnification projects the sternoclavicular joints to either side of the thoracic spine. The supine position is comfortable for the symptomatic patient. Motion studies are made.", "contents": "Symmetrical anteroposterior projections of the sternoclavicular joints with motion studies. A radiographic technique is described for the symmetrical visualization of the sternoclavicular joints in the anteroposterior projection. A short focal-spot patient distance is used with an ultra-fine focal-spot tube, so that differential magnification projects the sternoclavicular joints to either side of the thoracic spine. The supine position is comfortable for the symptomatic patient. Motion studies are made."}
{"id": "PMID:472270", "title": "Lateral C1-2 puncture for myelography: posterior approach.", "content": "The lateral C1-2 puncture approach for myelography is a modification of the technique for percutaneous radiofrequency cervical cordotomy. Positioning of the needle in the posterior, rather than anterior, one third of the canal avoids injury to anomalous vertebral artery branches which lie in the anterior half of the neural canal at this level.", "contents": "Lateral C1-2 puncture for myelography: posterior approach. The lateral C1-2 puncture approach for myelography is a modification of the technique for percutaneous radiofrequency cervical cordotomy. Positioning of the needle in the posterior, rather than anterior, one third of the canal avoids injury to anomalous vertebral artery branches which lie in the anterior half of the neural canal at this level."}
{"id": "PMID:472271", "title": "Balloon catheters for investigating carotid cavernous fistulas.", "content": "A simple and reliable technique is described for investigating the precise location of an arteriovenous fistulous communication and its hemodynamics by temporary and complete vessel occlusion with a double-lumen balloon catheter.", "contents": "Balloon catheters for investigating carotid cavernous fistulas. A simple and reliable technique is described for investigating the precise location of an arteriovenous fistulous communication and its hemodynamics by temporary and complete vessel occlusion with a double-lumen balloon catheter."}
{"id": "PMID:472272", "title": "An isocentric technique for treatment of carcinoma of the prostate in large patients.", "content": "An isocentric technique for treatment of carcinoma of the prostate of a large patient was investigated for a 4-MV linear accelerator. A split course, two four-field technique was found to deliver an appropriate high-dose zone while not exceeding tissue tolerances.", "contents": "An isocentric technique for treatment of carcinoma of the prostate in large patients. An isocentric technique for treatment of carcinoma of the prostate of a large patient was investigated for a 4-MV linear accelerator. A split course, two four-field technique was found to deliver an appropriate high-dose zone while not exceeding tissue tolerances."}
{"id": "PMID:472273", "title": "Scattered radiation from trimmer bars in the cobalt-60 teletherapy machines.", "content": "The amount of scattered radiation resulting from four Cobalt-60 teletherapy units in four different hospitals when trimmer bars were fully extended was studied. Scatter was present for all machines checked at between 10% and 20% of the central axis dose. Clinical significance is also discussed.", "contents": "Scattered radiation from trimmer bars in the cobalt-60 teletherapy machines. The amount of scattered radiation resulting from four Cobalt-60 teletherapy units in four different hospitals when trimmer bars were fully extended was studied. Scatter was present for all machines checked at between 10% and 20% of the central axis dose. Clinical significance is also discussed."}
{"id": "PMID:472277", "title": "Basioccipital clefts.", "content": "Two cases of rare basioccipital clefting are reported. In one patient, 10-year follow-up indicates that the radiologic appearance of the clefts does not change with time. Findings in the other case indicate that additional anomalies may occur in adjacent bone structures developing in sequence. A review of the embryological development and discussion of the radiological findings are presented.", "contents": "Basioccipital clefts. Two cases of rare basioccipital clefting are reported. In one patient, 10-year follow-up indicates that the radiologic appearance of the clefts does not change with time. Findings in the other case indicate that additional anomalies may occur in adjacent bone structures developing in sequence. A review of the embryological development and discussion of the radiological findings are presented."}
{"id": "PMID:472278", "title": "Evaluation of brain circulation by rapid rotational computed tomography.", "content": "Continuous rapid rotational CT brain scanning now permits the study of brain circulation. The parameters studied are related to the transit time of circulation and to blood flow differences within small portions of the brain. For ease of description, this study is called regional brain circulation (rBC). This preliminary report is based on our experience with 75 consecutive cases studied since August 1, 1978. Displays of normal brain, cerebral ischemia, and arteriovenous malformations are analyzed in this paper. The normal graphic display of rBC includes peak concentration, transit time, washin and washout phases. Clinical application to medical-surgical treatment is illustrated.", "contents": "Evaluation of brain circulation by rapid rotational computed tomography. Continuous rapid rotational CT brain scanning now permits the study of brain circulation. The parameters studied are related to the transit time of circulation and to blood flow differences within small portions of the brain. For ease of description, this study is called regional brain circulation (rBC). This preliminary report is based on our experience with 75 consecutive cases studied since August 1, 1978. Displays of normal brain, cerebral ischemia, and arteriovenous malformations are analyzed in this paper. The normal graphic display of rBC includes peak concentration, transit time, washin and washout phases. Clinical application to medical-surgical treatment is illustrated."}
{"id": "PMID:472279", "title": "Experimental brain abscess evolution: computed tomographic and neuropathologic correlation.", "content": "Brain abscess evolution was studied in dogs by correlating the CT appearance with the neuropathologic findings. The abscess, produced by direct inoculation, progressed from an area of cerebritis to a well encapsulated abscess over 14 days. Ring enhancement was seen in the cerebritis stage prior to capsule formation. The ring reached its maximum size at this stage and correlated best with the area of cerebritis surrounding the developing necrotic center; the rim of this ring increased in thickness, resulting in progressive diminution of the central lucent area on scans delayed up to 60 min. The diameter of the ring decreased as cerebritis receded. Once the capsule had formed, the central lucent area was similar and no longer filled in on delayed scans.", "contents": "Experimental brain abscess evolution: computed tomographic and neuropathologic correlation. Brain abscess evolution was studied in dogs by correlating the CT appearance with the neuropathologic findings. The abscess, produced by direct inoculation, progressed from an area of cerebritis to a well encapsulated abscess over 14 days. Ring enhancement was seen in the cerebritis stage prior to capsule formation. The ring reached its maximum size at this stage and correlated best with the area of cerebritis surrounding the developing necrotic center; the rim of this ring increased in thickness, resulting in progressive diminution of the central lucent area on scans delayed up to 60 min. The diameter of the ring decreased as cerebritis receded. Once the capsule had formed, the central lucent area was similar and no longer filled in on delayed scans."}
{"id": "PMID:472280", "title": "Atherosclerotic subintimal hematoma of the carotid artery.", "content": "A presumed new radiological-pathological entity of atherosclerotic subintimal of the carotid artery is described. Subintimal hematomas were found in 12 of 50 (24%) carotid bifurcations during surgery for repeated transient ischemic attacks in a single hemispheric distribution; only 33% were associated with ulcerations. The typical angiographic appearance was a sharply marginated, rounded, eccentric filling defect located near the extracranial carotid bifurcation, although occasionally it may simulate a typical smooth or even ulcerated atherosclerotic plaque. Pathological mechanisms responsible for the hemorrhage into the atherosclerotic plaque resulting in the hematoma are discussed.", "contents": "Atherosclerotic subintimal hematoma of the carotid artery. A presumed new radiological-pathological entity of atherosclerotic subintimal of the carotid artery is described. Subintimal hematomas were found in 12 of 50 (24%) carotid bifurcations during surgery for repeated transient ischemic attacks in a single hemispheric distribution; only 33% were associated with ulcerations. The typical angiographic appearance was a sharply marginated, rounded, eccentric filling defect located near the extracranial carotid bifurcation, although occasionally it may simulate a typical smooth or even ulcerated atherosclerotic plaque. Pathological mechanisms responsible for the hemorrhage into the atherosclerotic plaque resulting in the hematoma are discussed."}
{"id": "PMID:472281", "title": "A comparative evaluation of computed tomography and laryngography.", "content": "A prospective study of 66 patients was conducted to evaluate the relative merits of computed tomography (CT) and laryngography in the assessment of laryngeal disorders. CT complemented direct laryngoscopy and biopsy for treatment planning and was superior to both modalities for showing deep infiltration, invasion of cartilage, and extension to the soft tissues of the neck. The subglottic space can be studied adequately by CT, which could also be useful in diagnosis of lymph node metastases. Distortion of the thyroid cartilage can be shown by CT. CT was equal to or better than laryngography in approximately 90% of cases.", "contents": "A comparative evaluation of computed tomography and laryngography. A prospective study of 66 patients was conducted to evaluate the relative merits of computed tomography (CT) and laryngography in the assessment of laryngeal disorders. CT complemented direct laryngoscopy and biopsy for treatment planning and was superior to both modalities for showing deep infiltration, invasion of cartilage, and extension to the soft tissues of the neck. The subglottic space can be studied adequately by CT, which could also be useful in diagnosis of lymph node metastases. Distortion of the thyroid cartilage can be shown by CT. CT was equal to or better than laryngography in approximately 90% of cases."}
{"id": "PMID:472283", "title": "The role of computed tomography in the evaluation of neck masses.", "content": "Application of computed tomography (CT) to neck masses has received little attention. The authors reviewed 10 cervical masses studied with CT as well as conventional imaging modalities. CT was extremely useful in defining both the osseous and soft-tissue extent of the lesion. In several instances, CT was able to show the relationship of the tumor to the spinal canal. When combined with angiography, CT demonstrated the relationship of the major cervical vascular channels to the lesion. Pathological conditions included neurofibroma, chordoma, branchial cleft cyst, neuroblastoma, lymphoma, neurilemmoma, and metastatic carcinoma.", "contents": "The role of computed tomography in the evaluation of neck masses. Application of computed tomography (CT) to neck masses has received little attention. The authors reviewed 10 cervical masses studied with CT as well as conventional imaging modalities. CT was extremely useful in defining both the osseous and soft-tissue extent of the lesion. In several instances, CT was able to show the relationship of the tumor to the spinal canal. When combined with angiography, CT demonstrated the relationship of the major cervical vascular channels to the lesion. Pathological conditions included neurofibroma, chordoma, branchial cleft cyst, neuroblastoma, lymphoma, neurilemmoma, and metastatic carcinoma."}
{"id": "PMID:472282", "title": "Computed tomography of the injured larynx.", "content": "Computed tomography (CT) permitted a much more detailed appraisal of laryngeal dysfunction in patients with blunt laryngeal trauma (8 cases) and iatrogenic injury caused by radiation therapy (7 cases), surgery (2 cases), or intubation (1 case). In thyroid cartilage fractures, the fragments may be widely displaced. The cricoid ring breaks in two places, frequently involving the signet. CT facilitates diagnosis of the mechanism of injury based on the site and extent of fractures. The patterns of distortion and fibrosis of the laryngeal cartilage following radiation therapy may be manifested as encroachment on the airway, easily mistaken for recurrent tumor or localized edema. Minor distortion probably escapes detection on clinical examination. The authors consider CT the examination of choice in laryngeal injury.", "contents": "Computed tomography of the injured larynx. Computed tomography (CT) permitted a much more detailed appraisal of laryngeal dysfunction in patients with blunt laryngeal trauma (8 cases) and iatrogenic injury caused by radiation therapy (7 cases), surgery (2 cases), or intubation (1 case). In thyroid cartilage fractures, the fragments may be widely displaced. The cricoid ring breaks in two places, frequently involving the signet. CT facilitates diagnosis of the mechanism of injury based on the site and extent of fractures. The patterns of distortion and fibrosis of the laryngeal cartilage following radiation therapy may be manifested as encroachment on the airway, easily mistaken for recurrent tumor or localized edema. Minor distortion probably escapes detection on clinical examination. The authors consider CT the examination of choice in laryngeal injury."}
{"id": "PMID:472284", "title": "Computed tomographic staging of malignant gastric neoplasms.", "content": "Eight patients with proved gastric carcinoma and 3 with gastric lymphoma were studied preoperatively with computed tomography. CT accurately identified abnormal stomach wall thickening and intra-abdominal tumor extension. Mural thickening was seen on the CT scan when a moderately distended stomach had a wall more than 10 mm thick. CT was useful for assessing surgical resectability, evaluating tumor response to chemotherapy or radiotherapy, and detecting postoperative recurrence. Fourteen patients with an abnormal barium study suggesting gastric malignancy were also studied with CT, which correctly demonstrated a normal stomach or identified the reason for the abnormal barium study, such as an unusual placed spleen or a pancreatic carcinoma invading the stomach.", "contents": "Computed tomographic staging of malignant gastric neoplasms. Eight patients with proved gastric carcinoma and 3 with gastric lymphoma were studied preoperatively with computed tomography. CT accurately identified abnormal stomach wall thickening and intra-abdominal tumor extension. Mural thickening was seen on the CT scan when a moderately distended stomach had a wall more than 10 mm thick. CT was useful for assessing surgical resectability, evaluating tumor response to chemotherapy or radiotherapy, and detecting postoperative recurrence. Fourteen patients with an abnormal barium study suggesting gastric malignancy were also studied with CT, which correctly demonstrated a normal stomach or identified the reason for the abnormal barium study, such as an unusual placed spleen or a pancreatic carcinoma invading the stomach."}
{"id": "PMID:472285", "title": "The accuracy of sonography in the differential diagnosis of obstructive jaundice: a comparison with cholangiography.", "content": "The value of sonography in determining the site and nature of biliary obstruction with surgical jaundice is described in 32 patients. All diagnoses were subsequently established by surgery and pathology. The site of obstruction was delineated in 94%, while the nature of the lesion was established in 81%. In comparison, contrast cholangiography correctly delineated the site in 96%, but helped determine the etiology in only 82%. Sonography is sufficient to adequately evaluate patients prior to surgery, while percutaneous transhepatic cholangiography should probably be used only when satisfactory ultrasound examinations cannot be obtained.", "contents": "The accuracy of sonography in the differential diagnosis of obstructive jaundice: a comparison with cholangiography. The value of sonography in determining the site and nature of biliary obstruction with surgical jaundice is described in 32 patients. All diagnoses were subsequently established by surgery and pathology. The site of obstruction was delineated in 94%, while the nature of the lesion was established in 81%. In comparison, contrast cholangiography correctly delineated the site in 96%, but helped determine the etiology in only 82%. Sonography is sufficient to adequately evaluate patients prior to surgery, while percutaneous transhepatic cholangiography should probably be used only when satisfactory ultrasound examinations cannot be obtained."}
{"id": "PMID:472286", "title": "Ultrasonography and computed tomography of carcinoma of the gallbladder.", "content": "Ultrasonography and CT have been used to diagnose carcinoma of the gallbladder, since in most patients the diagnosis cannot be made through radiography alone. Ultrasonography was performed on 14 patients and CT on 7. Ultrasonographic findings are classified into four types. Frequently, there are gallstones, dilated bile ducts, or metastatic lesions in the liver or retroperitoneal lymph nodes. The diagnosis was made or suggested by ultrasonography in 84.6% of patients. Findings on CT correlated well with the ultrasonographic features.", "contents": "Ultrasonography and computed tomography of carcinoma of the gallbladder. Ultrasonography and CT have been used to diagnose carcinoma of the gallbladder, since in most patients the diagnosis cannot be made through radiography alone. Ultrasonography was performed on 14 patients and CT on 7. Ultrasonographic findings are classified into four types. Frequently, there are gallstones, dilated bile ducts, or metastatic lesions in the liver or retroperitoneal lymph nodes. The diagnosis was made or suggested by ultrasonography in 84.6% of patients. Findings on CT correlated well with the ultrasonographic features."}
{"id": "PMID:472287", "title": "Air crescent sign of invasive aspergillosis.", "content": "Pulmonary infection in immunocompromised patients is frequently difficult to diagnose. Therapy for the more common pathogens differs greatly from that for infection with unusual opportunistic organisms. However, neither of these infectious agents offers specific radiographic signs. The authors report on 4 patients with acute leukemia and invasive aspergillosis whose radiographs demonstrated a distinctive feature of one or more air crescents within an area of pulmonary infiltrate. Autopsy studies correlated the radiographic changes with an infection due to Aspergillus species fungi. While the sign is not pathognomonic for Aspergillus infection, seen in a suitable host, it would suggest the possibility of invasive aspergillosis.", "contents": "Air crescent sign of invasive aspergillosis. Pulmonary infection in immunocompromised patients is frequently difficult to diagnose. Therapy for the more common pathogens differs greatly from that for infection with unusual opportunistic organisms. However, neither of these infectious agents offers specific radiographic signs. The authors report on 4 patients with acute leukemia and invasive aspergillosis whose radiographs demonstrated a distinctive feature of one or more air crescents within an area of pulmonary infiltrate. Autopsy studies correlated the radiographic changes with an infection due to Aspergillus species fungi. While the sign is not pathognomonic for Aspergillus infection, seen in a suitable host, it would suggest the possibility of invasive aspergillosis."}
{"id": "PMID:472288", "title": "Cellular attachment as a sensitive indicator of the effects of diagnostic ultrasound exposure on cultured human cells.", "content": "Dispersed cultured human cells (T-1 kidney, FL embryonic kidney, ES amniotic, and JHA amniotic) were seeded into plastic Petri dishes, incubated for 45 minutes, exposed to a clinical ultrasound source [total power output, 1.76 +/- 0.18 (S.D.) mW] for 0.25-60 minutes, and the medium replaced. Attachment was significantly reduced after only 0.50 minute of exposure. For all exposures, sensitivity of the JHA amniotic cells was greater than that of the kidney lines. Like incremental reduction in attachment, differential sensitivity among cell lines became less pronounced with protracted exposures. Thus cellular attachment is a sensitive parameter for studying the effects of diagnostic ultrasound.", "contents": "Cellular attachment as a sensitive indicator of the effects of diagnostic ultrasound exposure on cultured human cells. Dispersed cultured human cells (T-1 kidney, FL embryonic kidney, ES amniotic, and JHA amniotic) were seeded into plastic Petri dishes, incubated for 45 minutes, exposed to a clinical ultrasound source [total power output, 1.76 +/- 0.18 (S.D.) mW] for 0.25-60 minutes, and the medium replaced. Attachment was significantly reduced after only 0.50 minute of exposure. For all exposures, sensitivity of the JHA amniotic cells was greater than that of the kidney lines. Like incremental reduction in attachment, differential sensitivity among cell lines became less pronounced with protracted exposures. Thus cellular attachment is a sensitive parameter for studying the effects of diagnostic ultrasound."}
{"id": "PMID:472289", "title": "Sonographic morphology of the normal menstrual cycle.", "content": "Sequential gray-scale sonograms were obtained during 20 menstrual cycles in 16 normal female volunteers. Hormonal and physical parameters of an ovulatory cycle were correlated with morphological changes in the ovaries, uterus, and cul-de-sac as seen on the sonogram. Ovarian cysts of two sizes were observed, corresponding chronologically and morphologically to Graafian follicles and corpora lutea. Small amounts of free pelvic fluid were demonstrated in many women at ovulation. A characteristic uterine appearance is seen prior to menstruation and is related to hormonal influences on the uterus. These findings emphasize the importance of recognizing normal physiological changes when interpreting gynecologic sonograms.", "contents": "Sonographic morphology of the normal menstrual cycle. Sequential gray-scale sonograms were obtained during 20 menstrual cycles in 16 normal female volunteers. Hormonal and physical parameters of an ovulatory cycle were correlated with morphological changes in the ovaries, uterus, and cul-de-sac as seen on the sonogram. Ovarian cysts of two sizes were observed, corresponding chronologically and morphologically to Graafian follicles and corpora lutea. Small amounts of free pelvic fluid were demonstrated in many women at ovulation. A characteristic uterine appearance is seen prior to menstruation and is related to hormonal influences on the uterus. These findings emphasize the importance of recognizing normal physiological changes when interpreting gynecologic sonograms."}
{"id": "PMID:472290", "title": "Interpretation of indeterminate lung scintigrams.", "content": "Lung scintigrams and pulmonary angiograms of 111 patients with suspected pulmonary embolism who had matching perfusion defects and radiographic abnormalities (infiltrate, atelectasis, or effusion) were reviewed. In 14 patients perfusion defects were substantially smaller than the corresponding radiographic opacity; only 1 (7%) had pulmonary embolism. In 77 the opacities and perfusion defects were of similar size; the abnormality was due to embolism in 20 (26%). In 18 patients perfusion defects were substantially larger than the radiographic opacities and were not associated with matching ventilation abnormalities; of these, 16 (89%) had pulmonary embolism. Evaluation of the relative size of perfusion defects and radiographic abnormalities occurring in the same region improves the ability of lung scintigrams to predict pulmonary emboli.", "contents": "Interpretation of indeterminate lung scintigrams. Lung scintigrams and pulmonary angiograms of 111 patients with suspected pulmonary embolism who had matching perfusion defects and radiographic abnormalities (infiltrate, atelectasis, or effusion) were reviewed. In 14 patients perfusion defects were substantially smaller than the corresponding radiographic opacity; only 1 (7%) had pulmonary embolism. In 77 the opacities and perfusion defects were of similar size; the abnormality was due to embolism in 20 (26%). In 18 patients perfusion defects were substantially larger than the radiographic opacities and were not associated with matching ventilation abnormalities; of these, 16 (89%) had pulmonary embolism. Evaluation of the relative size of perfusion defects and radiographic abnormalities occurring in the same region improves the ability of lung scintigrams to predict pulmonary emboli."}
{"id": "PMID:472291", "title": "Positron-emission computed tomography of the pancreas: a preliminary study.", "content": "Twenty-seven patients with suspected pancreatic disease were examined with emission computed tomography (ECT) with 11C-labeled amino acids. Of 24 patients with known clinical outcome, there were 1 false positive and 2 false negative results. One finding of interest was the increased concentration of radiopharmaceutical that accurately delineated the presence and extent of 4 pancreatic carcinomas and 1 lymphoma. In the normal subjects ECT reliably identified the pancreas. Disadvantages of ECT include the need for a cyclotron and high-quality hot chemistry laboratory, and the relatively long scanning time. The advantage of ECT is that it offers a unique opportunity to image biologic activity of the pancreas.", "contents": "Positron-emission computed tomography of the pancreas: a preliminary study. Twenty-seven patients with suspected pancreatic disease were examined with emission computed tomography (ECT) with 11C-labeled amino acids. Of 24 patients with known clinical outcome, there were 1 false positive and 2 false negative results. One finding of interest was the increased concentration of radiopharmaceutical that accurately delineated the presence and extent of 4 pancreatic carcinomas and 1 lymphoma. In the normal subjects ECT reliably identified the pancreas. Disadvantages of ECT include the need for a cyclotron and high-quality hot chemistry laboratory, and the relatively long scanning time. The advantage of ECT is that it offers a unique opportunity to image biologic activity of the pancreas."}
{"id": "PMID:472292", "title": "Radionuclide imaging in the assessment of primary chronic pyelonephritis.", "content": "The diagnostic value of radionuclide imaging as a supplement to excretory urography was assessed in 33 patients with primary chronic pyelonephritis. Both 99mTc-glucoheptonate and 131I-Hippuran were used. Radionuclide imaging did not improve the sensitivity of detection by urography alone (31 of 33 patients). Nevertheless, in one third of cases the radionuclide studies demonstrated certain abnormalities more readily, including focal parenchymal damage, renal functional impairment, and decreased renal perfusion. Radionuclide imaging is useful when minimal or no abnormalities are observed on the excretory urogram or nephrotomogram.", "contents": "Radionuclide imaging in the assessment of primary chronic pyelonephritis. The diagnostic value of radionuclide imaging as a supplement to excretory urography was assessed in 33 patients with primary chronic pyelonephritis. Both 99mTc-glucoheptonate and 131I-Hippuran were used. Radionuclide imaging did not improve the sensitivity of detection by urography alone (31 of 33 patients). Nevertheless, in one third of cases the radionuclide studies demonstrated certain abnormalities more readily, including focal parenchymal damage, renal functional impairment, and decreased renal perfusion. Radionuclide imaging is useful when minimal or no abnormalities are observed on the excretory urogram or nephrotomogram."}
{"id": "PMID:472293", "title": "Bone marrow dose in chest radiography: the posteroanterior vs. anteroposterior projection.", "content": "The dose to active bone marrow resulting from anteroposterior (AP) and posteroanterior (PA) chest examinations was estimated using an Alderson Rando phantom and extruded lithium fluoride dosimeters. The AP projections resulted in a mean marrow dose range of 1.9-2.6 mrad (0.019-0.026 mGy) as compared to doses for PA projections of 3.4-3.8 mrad (0.034-0.038 mGy) for optimally diagnostic exposure taken at 70, 90, and 120 kVp.", "contents": "Bone marrow dose in chest radiography: the posteroanterior vs. anteroposterior projection. The dose to active bone marrow resulting from anteroposterior (AP) and posteroanterior (PA) chest examinations was estimated using an Alderson Rando phantom and extruded lithium fluoride dosimeters. The AP projections resulted in a mean marrow dose range of 1.9-2.6 mrad (0.019-0.026 mGy) as compared to doses for PA projections of 3.4-3.8 mrad (0.034-0.038 mGy) for optimally diagnostic exposure taken at 70, 90, and 120 kVp."}
{"id": "PMID:472294", "title": "Anthropomorphic test objects for CT scanners.", "content": "Numerous types of artifacts can result in clinical misinterpretations in computed tomography. The authors have evaluated phantom materials and techniques of construction which would produce objects similar enough to the human body to test the accuracy of CT in vivo. The object used for this purpose is a thoracic section with five soft-tissue materials, representing adipose tissue, muscle, heart, esophagus, and lung, and three bone materials representing cortical bone, average rib, and \"inner\" (trabecular) bone. The accuracy of CT numbers for studies of different organs was assessed and sources of error in five commercial scanners were identified.", "contents": "Anthropomorphic test objects for CT scanners. Numerous types of artifacts can result in clinical misinterpretations in computed tomography. The authors have evaluated phantom materials and techniques of construction which would produce objects similar enough to the human body to test the accuracy of CT in vivo. The object used for this purpose is a thoracic section with five soft-tissue materials, representing adipose tissue, muscle, heart, esophagus, and lung, and three bone materials representing cortical bone, average rib, and \"inner\" (trabecular) bone. The accuracy of CT numbers for studies of different organs was assessed and sources of error in five commercial scanners were identified."}
{"id": "PMID:472295", "title": "Magnification mammography: evaluation of screen-film and xeroradiographic techniques.", "content": "An x-ray unit designed for conventional nonmagnification and magnification mammography has been evaluated in terms of image quality and corresponding radiation exposure levels. The technical advantages of the radiographic magnification technique can result in improved image quality and reduction of the recording-system noise. The microfocal spot allows 1.5 x magnification mammograms with minimal geometric unsharpness. However, the magnification technique requires an increased radiation dose to the breast, compared to conventional nonmagnification techniques. An additional radiation dose may be required for screen-film magnification views because of reciprocity law failure due to long exposure times. The increased-dose limitation and the small dimensions of the recording-system cassettes have precluded the use of magnification in place of nonmagnified images for routine mammographic examination. The magnification technique has proved to be beneficial in selected cases.", "contents": "Magnification mammography: evaluation of screen-film and xeroradiographic techniques. An x-ray unit designed for conventional nonmagnification and magnification mammography has been evaluated in terms of image quality and corresponding radiation exposure levels. The technical advantages of the radiographic magnification technique can result in improved image quality and reduction of the recording-system noise. The microfocal spot allows 1.5 x magnification mammograms with minimal geometric unsharpness. However, the magnification technique requires an increased radiation dose to the breast, compared to conventional nonmagnification techniques. An additional radiation dose may be required for screen-film magnification views because of reciprocity law failure due to long exposure times. The increased-dose limitation and the small dimensions of the recording-system cassettes have precluded the use of magnification in place of nonmagnified images for routine mammographic examination. The magnification technique has proved to be beneficial in selected cases."}
{"id": "PMID:472296", "title": "The differential density sign of pericardial effusion.", "content": "Chest radiographs of 100 patients with suspected pericardial effusion who underwent echocardiography were reviewed. Fifty were echo-positive and 50 were negative. The differential density sign was present in 34 patients (68%) who were echo-positive and 2 patients (4%) who were negative; on frontal (62%) and lateral (41%) projections; and in patients with small, moderate, and large effusions. Computed tomographic findings in 1 patient correlated closely with those obtained radiographically. Experimental models suggest this sign is related to different tissue attenuation coefficients for blood and saline, different tissue thicknesses of the spherically shaped heart, the presence of epicardial fat around the heart, and chest film exposure factors. \"Trapping\" of the middle lobe or anterior lingula may also contribute. When pericardial effusion is not suspected, the presence of this sign should be an indication for echocardiography.", "contents": "The differential density sign of pericardial effusion. Chest radiographs of 100 patients with suspected pericardial effusion who underwent echocardiography were reviewed. Fifty were echo-positive and 50 were negative. The differential density sign was present in 34 patients (68%) who were echo-positive and 2 patients (4%) who were negative; on frontal (62%) and lateral (41%) projections; and in patients with small, moderate, and large effusions. Computed tomographic findings in 1 patient correlated closely with those obtained radiographically. Experimental models suggest this sign is related to different tissue attenuation coefficients for blood and saline, different tissue thicknesses of the spherically shaped heart, the presence of epicardial fat around the heart, and chest film exposure factors. \"Trapping\" of the middle lobe or anterior lingula may also contribute. When pericardial effusion is not suspected, the presence of this sign should be an indication for echocardiography."}
{"id": "PMID:472297", "title": "Digital radiograph of the chest using a computed tomography instrument.", "content": "Digital radiography (DR) was compared with conventional chest radiography in patients with mediastinal adenopathy, sarcoidosis, and metastatic adenocarcinoma. Contrast resolution was improved by the new technique, offering a wider latitude and edge enhancement. While small, low-contrast details were resolved best by conventional radiography, DR was superior for resolution of nodules. With further improvement of spatial and contrast resolution, DR could become the preferred technique of chest radiography.", "contents": "Digital radiograph of the chest using a computed tomography instrument. Digital radiography (DR) was compared with conventional chest radiography in patients with mediastinal adenopathy, sarcoidosis, and metastatic adenocarcinoma. Contrast resolution was improved by the new technique, offering a wider latitude and edge enhancement. While small, low-contrast details were resolved best by conventional radiography, DR was superior for resolution of nodules. With further improvement of spatial and contrast resolution, DR could become the preferred technique of chest radiography."}
{"id": "PMID:472298", "title": "Treatment of P388 leukemia in vivo with perfluoroctylbromide and radiation: a preliminary study.", "content": "Perfluorinated hydrocarbon compounds (PFHC) possess unique physical properties suggesting possible usefulness in man. Their low surface tension allows free flow into tiny folds and crevices, facilitating dispersion along peritoneal surfaces affected by a tumor. These substances are also oxygen solvents. BDF1 female mice injected intraperitoneally (IP) with 10(6) P388 mouse leukemia cells were studied following treatment with preoxygenated compound 1913 and increasing doses of radiation. Preliminary results suggest increased life span compared to radiation treatment alone. Radioenhancement at high sublethal doses of whole-body radiation cannot be excluded.", "contents": "Treatment of P388 leukemia in vivo with perfluoroctylbromide and radiation: a preliminary study. Perfluorinated hydrocarbon compounds (PFHC) possess unique physical properties suggesting possible usefulness in man. Their low surface tension allows free flow into tiny folds and crevices, facilitating dispersion along peritoneal surfaces affected by a tumor. These substances are also oxygen solvents. BDF1 female mice injected intraperitoneally (IP) with 10(6) P388 mouse leukemia cells were studied following treatment with preoxygenated compound 1913 and increasing doses of radiation. Preliminary results suggest increased life span compared to radiation treatment alone. Radioenhancement at high sublethal doses of whole-body radiation cannot be excluded."}
{"id": "PMID:472299", "title": "Renal radiography with cephalad angulation and inspiration.", "content": "Radiographs of the kidneys were obtained during inspiration with a 10 degrees cephalad angulation of the central ray. With this technique, costocartilaginous calcifications were not projected over the renal areas in 94% of cases.", "contents": "Renal radiography with cephalad angulation and inspiration. Radiographs of the kidneys were obtained during inspiration with a 10 degrees cephalad angulation of the central ray. With this technique, costocartilaginous calcifications were not projected over the renal areas in 94% of cases."}
{"id": "PMID:472300", "title": "A new device for barium-enema examination following colostomy.", "content": "The authors developed a colostomy appliance for use during barium-enema examinations. It is 8 cm in diameter and 4 cm in height, and is made of acrylic resins. With the use of this device, 21 patients were fluoroscopically examined through the stoma; good contrast views of the lower intestinal tract were obtained in all cases without leakage of barium or air.", "contents": "A new device for barium-enema examination following colostomy. The authors developed a colostomy appliance for use during barium-enema examinations. It is 8 cm in diameter and 4 cm in height, and is made of acrylic resins. With the use of this device, 21 patients were fluoroscopically examined through the stoma; good contrast views of the lower intestinal tract were obtained in all cases without leakage of barium or air."}
{"id": "PMID:472301", "title": "Percutaneous transhepatic gallstone removal by needle tract.", "content": "A percutaneous transhepatic cholangiogram tract was used to visualize a large stone in the common duct; following the tract's dilation, the stone was crushed and partially removed. Fragments were flushed and also passed spontaneously into the duodenum. The approach described offers a feasible alternative to surgery.", "contents": "Percutaneous transhepatic gallstone removal by needle tract. A percutaneous transhepatic cholangiogram tract was used to visualize a large stone in the common duct; following the tract's dilation, the stone was crushed and partially removed. Fragments were flushed and also passed spontaneously into the duodenum. The approach described offers a feasible alternative to surgery."}
{"id": "PMID:472302", "title": "Bacteriologic flora on diagnostic ultrasonic transducers.", "content": "In a study to determine the presence of bacteria on ultrasonic transducers, the authors found micro-organisms in 82% of cultures. The results show that 70% isopropyl alcohol is not a consistently effective germicide for the sterilization of transducers. The presence of bacteria, however, appears to be of little clinical consequence.", "contents": "Bacteriologic flora on diagnostic ultrasonic transducers. In a study to determine the presence of bacteria on ultrasonic transducers, the authors found micro-organisms in 82% of cultures. The results show that 70% isopropyl alcohol is not a consistently effective germicide for the sterilization of transducers. The presence of bacteria, however, appears to be of little clinical consequence."}
{"id": "PMID:472303", "title": "Separation of a femoral artery catheter during withdrawal.", "content": "A complication of separation of a femoral artery catheter during withdrawal is described. Probable contributing factors and ways of avoiding this mishap are discussed.", "contents": "Separation of a femoral artery catheter during withdrawal. A complication of separation of a femoral artery catheter during withdrawal is described. Probable contributing factors and ways of avoiding this mishap are discussed."}
{"id": "PMID:472304", "title": "Laser backpointer for use with a radiotherapy simulator.", "content": "A radiotherapy simulator with image intensification has been equipped with a laser backpointer which does not need to be removed during fluoroscopic examinations, provides negligible interference with the fluoroscopic image, and results in significant reductions in the time required for mark-up of patients. Accuracy of set-ups may be improved since rechecking of patient positioning has been simplified.", "contents": "Laser backpointer for use with a radiotherapy simulator. A radiotherapy simulator with image intensification has been equipped with a laser backpointer which does not need to be removed during fluoroscopic examinations, provides negligible interference with the fluoroscopic image, and results in significant reductions in the time required for mark-up of patients. Accuracy of set-ups may be improved since rechecking of patient positioning has been simplified."}
{"id": "PMID:472305", "title": "The effect of bone on dose distributions produced by the Fermi National Laboratory fast-neutron beam.", "content": "The dose distortion produced by bone during fast-neutron therapy was studied using a target-to-skin distance of 153 cm, with beams 10 x 10 field size at the surface of a phantom. Doses were measured using several muscle-equivalent ionization chambers in the phantom. Results showed that the dose was reduced by interposing bone-equivalent material between the point in question and the surface of the phantom.", "contents": "The effect of bone on dose distributions produced by the Fermi National Laboratory fast-neutron beam. The dose distortion produced by bone during fast-neutron therapy was studied using a target-to-skin distance of 153 cm, with beams 10 x 10 field size at the surface of a phantom. Doses were measured using several muscle-equivalent ionization chambers in the phantom. Results showed that the dose was reduced by interposing bone-equivalent material between the point in question and the surface of the phantom."}
{"id": "PMID:472306", "title": "Adaptation of multiformat film holder to real-time ultrasound.", "content": "A method is described for adaptation of a multiformat film holder to a free-standing real-time imaging unit. No additional electronics are necessary for modification.", "contents": "Adaptation of multiformat film holder to real-time ultrasound. A method is described for adaptation of a multiformat film holder to a free-standing real-time imaging unit. No additional electronics are necessary for modification."}
{"id": "PMID:472307", "title": "Screening is not diagnosis.", "content": "Mammography and physical examination, usually employed as diagnostic tools, may be used to screen for early detection of breast cancer. A study of these modalities used to aggressively screen patients in Cincinnati and Milwaukee is presented and compared to more traditional methods of breast cancer detection and diagnosis as done in Louisville. For a similar-sized group over the same period, the rate of cancers detected in Louisville did not exceed that in Cincinnati-Milwaukee. Aggressive screening will not increase the overall number of detected cancers but will decrease the number of advanced cancers. Mammography as a screening device is not in itself a diagnostic tool, since its potential benefit is maximized only through the use of nondiagnostic, indirect radiographic criteria.", "contents": "Screening is not diagnosis. Mammography and physical examination, usually employed as diagnostic tools, may be used to screen for early detection of breast cancer. A study of these modalities used to aggressively screen patients in Cincinnati and Milwaukee is presented and compared to more traditional methods of breast cancer detection and diagnosis as done in Louisville. For a similar-sized group over the same period, the rate of cancers detected in Louisville did not exceed that in Cincinnati-Milwaukee. Aggressive screening will not increase the overall number of detected cancers but will decrease the number of advanced cancers. Mammography as a screening device is not in itself a diagnostic tool, since its potential benefit is maximized only through the use of nondiagnostic, indirect radiographic criteria."}
{"id": "PMID:472308", "title": "Cinefluoroscopy in evaluating left ventricular contractility and aneurysms.", "content": "Cardiac cinefluoroscopy was performed immediately prior to biplane left ventriculography in 113 patients and the findings of two independent observers compared to the angiographically documented state of the left ventricle. The observers' interpretations were correct in 44% and 46% of studies, respectively; there were 41% and 24% false negatives and 24% and 29% false positives. The observers agreed in only 59% of cases. The results show that cinefluoroscopy for evaluation of left ventricular contractility is grossly inaccurate and should not be performed.", "contents": "Cinefluoroscopy in evaluating left ventricular contractility and aneurysms. Cardiac cinefluoroscopy was performed immediately prior to biplane left ventriculography in 113 patients and the findings of two independent observers compared to the angiographically documented state of the left ventricle. The observers' interpretations were correct in 44% and 46% of studies, respectively; there were 41% and 24% false negatives and 24% and 29% false positives. The observers agreed in only 59% of cases. The results show that cinefluoroscopy for evaluation of left ventricular contractility is grossly inaccurate and should not be performed."}
{"id": "PMID:472309", "title": "Transhepatic cholangiography: the radiological method of choice in suspected obstructive jaundice.", "content": "Twenty-five patients with severe jaundice were studied prospectively with computed tomography (CT), gray-scale ultrasonography (USG), and percutaneous transhepatic cholangiography (THC). Nineteen had obstruction of the biliary tree. The differentiation of obstructive from hepatic parenchymal causes of jaundice was 72% accurate with USG, 92% with CT, and 96% with THC. Biochemical studies when used alone were 72% accurate. Although the precise location of an obstructing lesion was determined in 12/19 cases by CT and 9/19 by USG, the cause was established in only 5 by CT and 7 by USG. THC was 100% successful in establishing both cause and site of obstructive jaundice without significant complications.", "contents": "Transhepatic cholangiography: the radiological method of choice in suspected obstructive jaundice. Twenty-five patients with severe jaundice were studied prospectively with computed tomography (CT), gray-scale ultrasonography (USG), and percutaneous transhepatic cholangiography (THC). Nineteen had obstruction of the biliary tree. The differentiation of obstructive from hepatic parenchymal causes of jaundice was 72% accurate with USG, 92% with CT, and 96% with THC. Biochemical studies when used alone were 72% accurate. Although the precise location of an obstructing lesion was determined in 12/19 cases by CT and 9/19 by USG, the cause was established in only 5 by CT and 7 by USG. THC was 100% successful in establishing both cause and site of obstructive jaundice without significant complications."}
{"id": "PMID:472310", "title": "Percutaneous nephrostomy in the management of ureteral and renal calculi.", "content": "A percutaneous nephrostomy tract can serve both to decompress the renal pelvis and as a route for dissolving renal stones and assisting in basket retrieval of ureteral stones. These techniques are especially valuable in patients who are poor operative risks.", "contents": "Percutaneous nephrostomy in the management of ureteral and renal calculi. A percutaneous nephrostomy tract can serve both to decompress the renal pelvis and as a route for dissolving renal stones and assisting in basket retrieval of ureteral stones. These techniques are especially valuable in patients who are poor operative risks."}
{"id": "PMID:472311", "title": "Axial skeletal changes in paraplegics.", "content": "Two-hundred paraplegic men were studied to determine the nature of their axial skeletal changes. Forty-two per cent had sacro-iliac (SI) joint changes which varied from marginal blurring, erosion, sclerosis, and narrowing of the joint space (9%) to complete obliteration of the joints (33%). Radiographs of the thoracolumbar spine revealed syndesmophytes (9%), interspinous ossification (2%), intervertebral disk calcification (1.5%), and large osteophytes (2%). These changes had no relation to race, age (except spine changes), or paralysis level, but were related to the duration of the paralysis. The observed skeletal changes mimic HLA-B27 arthropathy. However, the presence of para-osteoarthropathies and negative HLA-B27 in paraplegics would help in differential diagnosis.", "contents": "Axial skeletal changes in paraplegics. Two-hundred paraplegic men were studied to determine the nature of their axial skeletal changes. Forty-two per cent had sacro-iliac (SI) joint changes which varied from marginal blurring, erosion, sclerosis, and narrowing of the joint space (9%) to complete obliteration of the joints (33%). Radiographs of the thoracolumbar spine revealed syndesmophytes (9%), interspinous ossification (2%), intervertebral disk calcification (1.5%), and large osteophytes (2%). These changes had no relation to race, age (except spine changes), or paralysis level, but were related to the duration of the paralysis. The observed skeletal changes mimic HLA-B27 arthropathy. However, the presence of para-osteoarthropathies and negative HLA-B27 in paraplegics would help in differential diagnosis."}
{"id": "PMID:472312", "title": "Hypoplasia of the lumbar vertebral body simulating spondylolisthesis.", "content": "Fifty cases of spondylosis and spondylolisthesis were reviewed. In 9 of the 20 cases interpreted as Grade I spondylolisthesis at L5-S1, the sagittal diameter of L5 was shortened, creating a false impression of vertebral slippage. Such cases are felt to represent spondylolysis with hypoplasia of the vertebral body rather than true spondylolisthesis.", "contents": "Hypoplasia of the lumbar vertebral body simulating spondylolisthesis. Fifty cases of spondylosis and spondylolisthesis were reviewed. In 9 of the 20 cases interpreted as Grade I spondylolisthesis at L5-S1, the sagittal diameter of L5 was shortened, creating a false impression of vertebral slippage. Such cases are felt to represent spondylolysis with hypoplasia of the vertebral body rather than true spondylolisthesis."}
{"id": "PMID:472313", "title": "Volar plate fractures.", "content": "Rupture of the volar plate of the proximal interphalangeal joint, usually secondary to hyper-extension injury, may be accompanied by a radiographically evident avulsion fracture at the base of the middle phalanx. Findings were reviewed in 55 patients with 58 cases of volar plate fracture. This fracture should be specifically looked for in a patient with the typical history, since it may be the only radiographic evidence of a potentially disabling injury.", "contents": "Volar plate fractures. Rupture of the volar plate of the proximal interphalangeal joint, usually secondary to hyper-extension injury, may be accompanied by a radiographically evident avulsion fracture at the base of the middle phalanx. Findings were reviewed in 55 patients with 58 cases of volar plate fracture. This fracture should be specifically looked for in a patient with the typical history, since it may be the only radiographic evidence of a potentially disabling injury."}
{"id": "PMID:472314", "title": "Mammographic parenchymal patterns and risk of breast cancer.", "content": "A 15-year prospective study of mammographic parenchymal patterns in 7,123 women over 30 with 658 prevalent and 131 incident primary breast neoplasms demonstrated that no pattern is a reliable indicator of initial or developing cancer. Cancer in glandular breasts is less frequent, more difficult to detect by mammography, and tends to remain prevalent compared with the more frequent tumors in fatty breasts. As women get older, their breasts assume similar patterns and the incidence of both prevalent and incident tumors increases. Parenchymal patterns cannot signify which breasts may remain glandular, nor which women may be at risk of cancer.", "contents": "Mammographic parenchymal patterns and risk of breast cancer. A 15-year prospective study of mammographic parenchymal patterns in 7,123 women over 30 with 658 prevalent and 131 incident primary breast neoplasms demonstrated that no pattern is a reliable indicator of initial or developing cancer. Cancer in glandular breasts is less frequent, more difficult to detect by mammography, and tends to remain prevalent compared with the more frequent tumors in fatty breasts. As women get older, their breasts assume similar patterns and the incidence of both prevalent and incident tumors increases. Parenchymal patterns cannot signify which breasts may remain glandular, nor which women may be at risk of cancer."}
{"id": "PMID:472315", "title": "Venography of peripheral venous injuries.", "content": "Venography is a simple and rapid technique which can be used in the diagnosis of acute penetrating venous injury. Four cases of venous lesions are presented and indications for venography discussed. Findings consisted of bleeding from lacerations and partial or total occlusion from traumatic thrombosis.", "contents": "Venography of peripheral venous injuries. Venography is a simple and rapid technique which can be used in the diagnosis of acute penetrating venous injury. Four cases of venous lesions are presented and indications for venography discussed. Findings consisted of bleeding from lacerations and partial or total occlusion from traumatic thrombosis."}
{"id": "PMID:472316", "title": "Phlegmasia cerulea dolens.", "content": "Phlegmasia cerulea dolens is a severe form of venous thrombosis presenting as an edematous, painful, purplish-blue lower extremity. Its rapid recognition as venous thrombosis rather than arterial insufficiency is important in avoiding gangrene and other life threatening complications. Three cases illustrating the presentation of this disorder are reported.", "contents": "Phlegmasia cerulea dolens. Phlegmasia cerulea dolens is a severe form of venous thrombosis presenting as an edematous, painful, purplish-blue lower extremity. Its rapid recognition as venous thrombosis rather than arterial insufficiency is important in avoiding gangrene and other life threatening complications. Three cases illustrating the presentation of this disorder are reported."}
{"id": "PMID:472317", "title": "Digital radiography using a computed tomographic instrument.", "content": "A prototype computed radiography (CR) system was evaluated for its efficacy as an independent diagnostic modality. Preliminary measurements of high contrast resolution, low contrast perceptibility, and dose were obtained. Clinical examinations including skull, abdomen, liver, gallbladder, biliary system, spine, and extremities were performed as an adjunct to either computed tomography or CR. The data suggest that CR can be an effective diagnostic imaging modality by itself. Advantages over conventional radiography include high scatter rejection, low patient dose, wide dynamic range, and good low contrast sensitivity for large objects; disadvantages, its long exposure time and relatively poor high contrast spatial resolution.", "contents": "Digital radiography using a computed tomographic instrument. A prototype computed radiography (CR) system was evaluated for its efficacy as an independent diagnostic modality. Preliminary measurements of high contrast resolution, low contrast perceptibility, and dose were obtained. Clinical examinations including skull, abdomen, liver, gallbladder, biliary system, spine, and extremities were performed as an adjunct to either computed tomography or CR. The data suggest that CR can be an effective diagnostic imaging modality by itself. Advantages over conventional radiography include high scatter rejection, low patient dose, wide dynamic range, and good low contrast sensitivity for large objects; disadvantages, its long exposure time and relatively poor high contrast spatial resolution."}
{"id": "PMID:472318", "title": "Symmetry of bronchial angles in children.", "content": "The bronchial angles in children from birth to age 18 years were measured. Symmetry of angles was found to age 15 years. After that age, an aortic indentation was generally noted on the trachea with an increased left bronchial angle. Hence, the relatively equal incidence of right- and left-sided aspiration of foreign bodies in smaller children as opposed to adults.", "contents": "Symmetry of bronchial angles in children. The bronchial angles in children from birth to age 18 years were measured. Symmetry of angles was found to age 15 years. After that age, an aortic indentation was generally noted on the trachea with an increased left bronchial angle. Hence, the relatively equal incidence of right- and left-sided aspiration of foreign bodies in smaller children as opposed to adults."}
{"id": "PMID:472319", "title": "Mucoid impaction (inspissated secretions) in segmental bronchial obstruction.", "content": "Localized persistent mucoid impaction as seen on the chest radiograph is a fairly common roentgen finding that always implies segmental bronchial obstruction. Collateral ventilation apparently permits air to enter the lung distal to the obstruction, outlining bronchi distended with trapped mucus or other secretions. This mucoid impaction sign has been seen in a variety of obstructing conditions, including primary and metastatic carcinoma of the lung, bronchial adenoma, tuberculous bronchostenosis, bronchial adenoma, tuberculous bronchostenosis, broncholithiasis, bronchial atresia, sequestration, pulmonary bronchogenic cyst, and foreign body. The roentgen appearance of the impacted mucus is variable, depending to a considerable degree on its orientation in relation to the direction of the x-ray beam. The presence of unexplained localized mucoid impaction is an indication for immediate study to demonstrate or exclude an obstructing bronchial lesion.", "contents": "Mucoid impaction (inspissated secretions) in segmental bronchial obstruction. Localized persistent mucoid impaction as seen on the chest radiograph is a fairly common roentgen finding that always implies segmental bronchial obstruction. Collateral ventilation apparently permits air to enter the lung distal to the obstruction, outlining bronchi distended with trapped mucus or other secretions. This mucoid impaction sign has been seen in a variety of obstructing conditions, including primary and metastatic carcinoma of the lung, bronchial adenoma, tuberculous bronchostenosis, bronchial adenoma, tuberculous bronchostenosis, broncholithiasis, bronchial atresia, sequestration, pulmonary bronchogenic cyst, and foreign body. The roentgen appearance of the impacted mucus is variable, depending to a considerable degree on its orientation in relation to the direction of the x-ray beam. The presence of unexplained localized mucoid impaction is an indication for immediate study to demonstrate or exclude an obstructing bronchial lesion."}
{"id": "PMID:472320", "title": "Hypochondroplasia: clinical and radiological aspects in 39 cases.", "content": "A retrospective review of 39 cases of hypochondroplasia revealed consistent but variable degrees of clinical and radiological features sufficient to allow differentiation from other well-established metaphyseal chondrodysplasias. Macrocephaly was noted in approximately half the cases. This contradicts a finding of normocephaly in previous studies.", "contents": "Hypochondroplasia: clinical and radiological aspects in 39 cases. A retrospective review of 39 cases of hypochondroplasia revealed consistent but variable degrees of clinical and radiological features sufficient to allow differentiation from other well-established metaphyseal chondrodysplasias. Macrocephaly was noted in approximately half the cases. This contradicts a finding of normocephaly in previous studies."}
{"id": "PMID:472331", "title": "The role of renal metabolism of PGE2 in determining its activity as a renal vasodilator in the dog.", "content": "Since the mammalian renal cortex avidly metabolizes prostaglandin E2 (PGE2), we examined the importance of renal metabolism of PGE2 in determining its renal vascular activity in the dog. We used 13, 14 dihydro PGE2 (DHPGE2) as a model compound to study this because DHPGE2 retains similar activity to the parent prostaglandin, PGE2, but is a poorer substrate than PGE2 for both the metabolism and the cellular uptake of the prostaglandins. Using dog renal cortical slices, we found that under similar experimental conditions, PGE2 was metabolized several-fold faster than DHPGE2. Both prostaglandins were metabolized to the 15 keto 13, 14 dihydro PGE2, which was positively identified using GC-MS. In vivo, we infused increasing concentrations of DHPGE2 into the renal artery of dogs and measured renal hemodynamic changes using radioactive microspheres. DHPGE2 was a potent renal vasodilator beginning at an infusion rate of 10(-9)g/kg/min. When compared to PGE2, DHPGE2 was about 10 times more potent in affecting renal vasodilation. The intrarenal redistribution of blood flow towards the inner cortex seen with DHPGE2 was identical to that seen with PGE2. We conclude that renal catabolism of PGE2 is very important in limiting the in vivo biological activity of PGE2, but regional differences in metabolism of PGE2 within the cortex are an unlikely determinant of the pattern of redistribution of renal blood flow.", "contents": "The role of renal metabolism of PGE2 in determining its activity as a renal vasodilator in the dog. Since the mammalian renal cortex avidly metabolizes prostaglandin E2 (PGE2), we examined the importance of renal metabolism of PGE2 in determining its renal vascular activity in the dog. We used 13, 14 dihydro PGE2 (DHPGE2) as a model compound to study this because DHPGE2 retains similar activity to the parent prostaglandin, PGE2, but is a poorer substrate than PGE2 for both the metabolism and the cellular uptake of the prostaglandins. Using dog renal cortical slices, we found that under similar experimental conditions, PGE2 was metabolized several-fold faster than DHPGE2. Both prostaglandins were metabolized to the 15 keto 13, 14 dihydro PGE2, which was positively identified using GC-MS. In vivo, we infused increasing concentrations of DHPGE2 into the renal artery of dogs and measured renal hemodynamic changes using radioactive microspheres. DHPGE2 was a potent renal vasodilator beginning at an infusion rate of 10(-9)g/kg/min. When compared to PGE2, DHPGE2 was about 10 times more potent in affecting renal vasodilation. The intrarenal redistribution of blood flow towards the inner cortex seen with DHPGE2 was identical to that seen with PGE2. We conclude that renal catabolism of PGE2 is very important in limiting the in vivo biological activity of PGE2, but regional differences in metabolism of PGE2 within the cortex are an unlikely determinant of the pattern of redistribution of renal blood flow."}
{"id": "PMID:472332", "title": "The effect of glucose on the synthesis of prostaglandins by the renal papilla of the rat in vitro.", "content": "Renal pappillae from rats were incubated in vitro. The release of prostaglandin by this tissue was found to be inversely related to the glucose concentration of the buffer. Estimates of prostaglandin release were determined by a rat stomach strip bioassay, and in some instances, gas-chromatography and mass spectrometry. When incubated in the presence of C14-arachidonic acid, the specific activity of prostaglandins E2 and F2 alpha released by the tissue was lower at the lower glucose concentration. Provision of 625 micrograms/ml of exogenous arachidonic acid in the buffer obliterated the effect of glucose on prostalglandin release. These data indicate that increasing amounts of glucose suppresses prostaglandin synthesis in the renal papilla of the rat, and that the mechanism of this phenomenon is related to the release of arachidonic acid from its storage pools in tissue lipids.", "contents": "The effect of glucose on the synthesis of prostaglandins by the renal papilla of the rat in vitro. Renal pappillae from rats were incubated in vitro. The release of prostaglandin by this tissue was found to be inversely related to the glucose concentration of the buffer. Estimates of prostaglandin release were determined by a rat stomach strip bioassay, and in some instances, gas-chromatography and mass spectrometry. When incubated in the presence of C14-arachidonic acid, the specific activity of prostaglandins E2 and F2 alpha released by the tissue was lower at the lower glucose concentration. Provision of 625 micrograms/ml of exogenous arachidonic acid in the buffer obliterated the effect of glucose on prostalglandin release. These data indicate that increasing amounts of glucose suppresses prostaglandin synthesis in the renal papilla of the rat, and that the mechanism of this phenomenon is related to the release of arachidonic acid from its storage pools in tissue lipids."}
{"id": "PMID:472333", "title": "Structure and quantitative determination of the major urinary metabolite of thromboxane B2 in the guinea pig.", "content": "2,3-Dinor-thromboxane B2 was the major urinary metabolite of thromboxane B2 in the guinea pig. The structure was assessed mainly by mass spectrometric analysis of a number of derivatives of the metabolite and by chemical degradation by oxidative ozonolysis. A method for quantitative determination of 2,3-dinor-thromboxane B2 in guinea pig urine based on multiple ion analysis and octadeuterated 2,3-dinor-thromboxane B2 as internal standard was developed. The basal excretion of the metabolite was 65 +/- 36 (S.D.) ng/kg x 24 h (n = 19; range 19--140 ng). This level corresponded to an endogenous synthesis of 543 +/- 300 ng of TXB2. No increase in the excretion was seen after anaphylaxis, in contrast to what has earlier been reported for PGF2 alpha.", "contents": "Structure and quantitative determination of the major urinary metabolite of thromboxane B2 in the guinea pig. 2,3-Dinor-thromboxane B2 was the major urinary metabolite of thromboxane B2 in the guinea pig. The structure was assessed mainly by mass spectrometric analysis of a number of derivatives of the metabolite and by chemical degradation by oxidative ozonolysis. A method for quantitative determination of 2,3-dinor-thromboxane B2 in guinea pig urine based on multiple ion analysis and octadeuterated 2,3-dinor-thromboxane B2 as internal standard was developed. The basal excretion of the metabolite was 65 +/- 36 (S.D.) ng/kg x 24 h (n = 19; range 19--140 ng). This level corresponded to an endogenous synthesis of 543 +/- 300 ng of TXB2. No increase in the excretion was seen after anaphylaxis, in contrast to what has earlier been reported for PGF2 alpha."}
{"id": "PMID:472334", "title": "Interaction between phenylephrine and prostaglandins E1 and F1 alpha on the contractile responses of vascular muscle.", "content": "Prostaglandins (PGs) E1 or F1 alpha (1.4--8.4 x 10(-8) M) contracted strips of rabbit aorta and increased the contractions produced by 1--6 x 10(-7) M phenylephrine (PE). The addition of the PGs simultaneously with PE or after a low concentration of PE (2 x 10(-7) M) significantly increased the PE-induced contractions. However, when the PGs were added after a higher concentration of PE (6 x 10(-7) M) an additional increase in the PE-induced contraction was produced with PGF1 alpha but not with PGE1. Isobolic plots of the data obtained from the simultaneous addition of PE and the PGs indicate that both PGs interact with PE in a synergistic or potentiative manner, suggesting that their effects are mediated through different receptor mechanisms. Addition of the PGs after a high dose of PE indicates that there may also be either qualitative or quantitative differences between PGE1 and PGF1 alpha.", "contents": "Interaction between phenylephrine and prostaglandins E1 and F1 alpha on the contractile responses of vascular muscle. Prostaglandins (PGs) E1 or F1 alpha (1.4--8.4 x 10(-8) M) contracted strips of rabbit aorta and increased the contractions produced by 1--6 x 10(-7) M phenylephrine (PE). The addition of the PGs simultaneously with PE or after a low concentration of PE (2 x 10(-7) M) significantly increased the PE-induced contractions. However, when the PGs were added after a higher concentration of PE (6 x 10(-7) M) an additional increase in the PE-induced contraction was produced with PGF1 alpha but not with PGE1. Isobolic plots of the data obtained from the simultaneous addition of PE and the PGs indicate that both PGs interact with PE in a synergistic or potentiative manner, suggesting that their effects are mediated through different receptor mechanisms. Addition of the PGs after a high dose of PE indicates that there may also be either qualitative or quantitative differences between PGE1 and PGF1 alpha."}
{"id": "PMID:472335", "title": "Central cardiovascular and thermal effects of prostaglandin F2 alpha in rats.", "content": "Administration of PGF2 ALPHA (0.2--6.4 micrograms) into the lateral cerebral ventricle (i.c.v.) induced dose-dependent increases in blood pressure, heart rate and body temperature in urethane-anaesthetised rats, but had no effect on these parameters when the same dose range was administered intravenously. Peripheral pretreatment with sodium meclofenamate (50 mg/kg s.c.) shifted all the dose-response curves for PGF2 alpha (i.c.v.) to the left, but indomethacin (50 mg/kg s.c.) did not significantly affect those changes. Central pretreatment with sodium meclofenamate or indomethacin (1.25 mg per rat i.c.v.) failed to modify significantly the effects of centrally administered PGF2 alpha. The results support previous suggestions that PGF2 alpha may participate in the central control of the cardiovascular and thermoregulatory systems, and also suggest that there may be differences in the sites and/or modes of action between sodium meclofenamate and indomethacin.", "contents": "Central cardiovascular and thermal effects of prostaglandin F2 alpha in rats. Administration of PGF2 ALPHA (0.2--6.4 micrograms) into the lateral cerebral ventricle (i.c.v.) induced dose-dependent increases in blood pressure, heart rate and body temperature in urethane-anaesthetised rats, but had no effect on these parameters when the same dose range was administered intravenously. Peripheral pretreatment with sodium meclofenamate (50 mg/kg s.c.) shifted all the dose-response curves for PGF2 alpha (i.c.v.) to the left, but indomethacin (50 mg/kg s.c.) did not significantly affect those changes. Central pretreatment with sodium meclofenamate or indomethacin (1.25 mg per rat i.c.v.) failed to modify significantly the effects of centrally administered PGF2 alpha. The results support previous suggestions that PGF2 alpha may participate in the central control of the cardiovascular and thermoregulatory systems, and also suggest that there may be differences in the sites and/or modes of action between sodium meclofenamate and indomethacin."}
{"id": "PMID:472336", "title": "Determination of prostaglandin F2 alpha, E2, D2 and 6-keto-F1 alpha in human cerebrospinal fluid.", "content": "Prostaglandin (PG)F2 alpha, E2, D2 and 6-keto-F1 ALha were determined in human cerebrospinal fluid by a mass spectrometric technique. The samples were obtained from 12 patients with suspected intracranial disease. A 64 fold variation in PG levels was observed. The major PG was 6-keto-F1 alpha (0.12--15 ng/ml). PGF2 alpha and PGE2 were present in lower concentrations PGD2 was below the level of detection (0.05 ng/ml) except in one patient with extremely high total levels of PGs.", "contents": "Determination of prostaglandin F2 alpha, E2, D2 and 6-keto-F1 alpha in human cerebrospinal fluid. Prostaglandin (PG)F2 alpha, E2, D2 and 6-keto-F1 ALha were determined in human cerebrospinal fluid by a mass spectrometric technique. The samples were obtained from 12 patients with suspected intracranial disease. A 64 fold variation in PG levels was observed. The major PG was 6-keto-F1 alpha (0.12--15 ng/ml). PGF2 alpha and PGE2 were present in lower concentrations PGD2 was below the level of detection (0.05 ng/ml) except in one patient with extremely high total levels of PGs."}
{"id": "PMID:472337", "title": "Regional and species differences in endogenous prostaglandin biosynthesis by brain homogenates.", "content": "Endogenously formed prostaglandins (PGs) D2, E2 and F2 alpha were determined in homogenates of brain regions from rat, guinea-pig, rabbit and cat, using gas-chromatography-mass spectrometry. The main PGs formed in the brain regions of the rat were PGD2, in the guinea-pig PGD2 and PGF2 alpha, in the rabbit PGF2 alpha and in the cat PGE2. Brain regions from the same animal species showed the same pattern of PG formation. They varied, however, in the amount of total PGs formed, the limbic system and the cerebral cortex being highest and cerebellum lowest.", "contents": "Regional and species differences in endogenous prostaglandin biosynthesis by brain homogenates. Endogenously formed prostaglandins (PGs) D2, E2 and F2 alpha were determined in homogenates of brain regions from rat, guinea-pig, rabbit and cat, using gas-chromatography-mass spectrometry. The main PGs formed in the brain regions of the rat were PGD2, in the guinea-pig PGD2 and PGF2 alpha, in the rabbit PGF2 alpha and in the cat PGE2. Brain regions from the same animal species showed the same pattern of PG formation. They varied, however, in the amount of total PGs formed, the limbic system and the cerebral cortex being highest and cerebellum lowest."}
{"id": "PMID:472338", "title": "Indomethacin increases abortifacient effect of PGE2 in man.", "content": "Results of this study indicate that pretreatment with indomethacin significantly increases the abortifacient effect of an intravenous infusion of PGE2 in patients admitted for abortion as a result of fetal death in utero. Indomethacin pretreatment shortened the duration of PGE2-induced abortion in primigravid and multigravid groups of patients by about 4 and 2 hours respectively. When primigravid and multigravid groups were combined, the dose of PGE2 needed for complete delivery decreases in the indomethacin-treated group by 39.9%.", "contents": "Indomethacin increases abortifacient effect of PGE2 in man. Results of this study indicate that pretreatment with indomethacin significantly increases the abortifacient effect of an intravenous infusion of PGE2 in patients admitted for abortion as a result of fetal death in utero. Indomethacin pretreatment shortened the duration of PGE2-induced abortion in primigravid and multigravid groups of patients by about 4 and 2 hours respectively. When primigravid and multigravid groups were combined, the dose of PGE2 needed for complete delivery decreases in the indomethacin-treated group by 39.9%."}
{"id": "PMID:472339", "title": "Intramuscular 16-phenoxy PGE2 ester for pregnancy termination.", "content": "A new PGE2 derivative (16-phenoxy PGE2 methyl sulfonylamide sulprostone) was administered by the i.m. route to 48 women pregnancy in any of the three trimesters. The indications for pregnancy interruption were either serious medical problems in intact pregnancies (21 cases) or due to fetal death in utero (27 cases). Single doses of 500 micrograms were repeated every 4 hours in the former group or every 6 hours in the latter category for a maximum period of 24 hours. The treatment was successful in 81% of intact pregnancies and in 92.6% of fetal death cases with an overall mean induction interval of 12.9 hours. More than half the subjects did not experience any side effects apart from mild or moderate uterine colics. An overall mean of 1.4 episodes of vomiting or diarrhoea per induction trial was quite acceptable from the clinical point of view. The absence of serious complications in the group of critically sick women speaks in favor of the relative safety of the drug.", "contents": "Intramuscular 16-phenoxy PGE2 ester for pregnancy termination. A new PGE2 derivative (16-phenoxy PGE2 methyl sulfonylamide sulprostone) was administered by the i.m. route to 48 women pregnancy in any of the three trimesters. The indications for pregnancy interruption were either serious medical problems in intact pregnancies (21 cases) or due to fetal death in utero (27 cases). Single doses of 500 micrograms were repeated every 4 hours in the former group or every 6 hours in the latter category for a maximum period of 24 hours. The treatment was successful in 81% of intact pregnancies and in 92.6% of fetal death cases with an overall mean induction interval of 12.9 hours. More than half the subjects did not experience any side effects apart from mild or moderate uterine colics. An overall mean of 1.4 episodes of vomiting or diarrhoea per induction trial was quite acceptable from the clinical point of view. The absence of serious complications in the group of critically sick women speaks in favor of the relative safety of the drug."}
{"id": "PMID:472340", "title": "Treatment with a single vaginal suppository containing 15-methyl PGF2 alpha methyl ester at expected time of menstruation.", "content": "Termination of early pregnancy, by vaginal administration of prostaglandin analogues, one to three weeks after the first missed menstrual period, has advantages and disadvantages in comparison with vacuum aspiration. Some of these may be reduced if the patient is treated earlier. In the present study the effect and safety of one vaginal administration of 2.5 to 3 mg 15-methyl-PGF2 alpha methyl ester around the expected time of menstruation was evaluated in 16 women exposed to the risk of pregnancy. The overall number of treatment cycles was 35 and pregnancy was confirmed by plasma beta-HCG in eight. The treatment resulted in bleeding in all the pregnant cycles while in the nonpregnant ones it only provoked spotting and bleeding did not begin until the expected time of menstruation. Treatment with 2.5 mg 15-methyl-PGF2 alpha methyl ester resulted in complete abortion in one of three women. If the dose was increased to 3 mg all five treated women aborted. In nonpregnant patients no changes in the levels of estradiol-17 beta or progesterone at any time during the 24-hour observation period were found. Serum cortisol and prolactin but not TSH levels started to increase two hours after the start of treatment and reached a maximum after five hours. The increase coincided with the onset of uterine pain. Ovulatory cycles as judged from basal body temperature occurred in the first menstrual cycle following treatment in all nonpregnant patients. Although possible to use as a \"once a month treatment\" it seems preferable since the dose is the same, to postpone treatment until menstruation is delayed for a week or more.", "contents": "Treatment with a single vaginal suppository containing 15-methyl PGF2 alpha methyl ester at expected time of menstruation. Termination of early pregnancy, by vaginal administration of prostaglandin analogues, one to three weeks after the first missed menstrual period, has advantages and disadvantages in comparison with vacuum aspiration. Some of these may be reduced if the patient is treated earlier. In the present study the effect and safety of one vaginal administration of 2.5 to 3 mg 15-methyl-PGF2 alpha methyl ester around the expected time of menstruation was evaluated in 16 women exposed to the risk of pregnancy. The overall number of treatment cycles was 35 and pregnancy was confirmed by plasma beta-HCG in eight. The treatment resulted in bleeding in all the pregnant cycles while in the nonpregnant ones it only provoked spotting and bleeding did not begin until the expected time of menstruation. Treatment with 2.5 mg 15-methyl-PGF2 alpha methyl ester resulted in complete abortion in one of three women. If the dose was increased to 3 mg all five treated women aborted. In nonpregnant patients no changes in the levels of estradiol-17 beta or progesterone at any time during the 24-hour observation period were found. Serum cortisol and prolactin but not TSH levels started to increase two hours after the start of treatment and reached a maximum after five hours. The increase coincided with the onset of uterine pain. Ovulatory cycles as judged from basal body temperature occurred in the first menstrual cycle following treatment in all nonpregnant patients. Although possible to use as a \"once a month treatment\" it seems preferable since the dose is the same, to postpone treatment until menstruation is delayed for a week or more."}
{"id": "PMID:472352", "title": "Manic-depressive illness in three generations of one family: a case study.", "content": "Two brothers presented for simultaneous admission for acute episodes of manic-depressive illness. Both had hospitalizations documented in the past for acute episodes. A family tree was constructed based on interviews with them as well as other family members and it was determined retrospectively that their father had a history of manic-depressive illness. Furthermore, the son of one of the brothers was also found to the affected. The findings for this family are inconsistent with the theory of X-linked inheritance of manic-depressive illness and adds to the growing criticism of that theory.", "contents": "Manic-depressive illness in three generations of one family: a case study. Two brothers presented for simultaneous admission for acute episodes of manic-depressive illness. Both had hospitalizations documented in the past for acute episodes. A family tree was constructed based on interviews with them as well as other family members and it was determined retrospectively that their father had a history of manic-depressive illness. Furthermore, the son of one of the brothers was also found to the affected. The findings for this family are inconsistent with the theory of X-linked inheritance of manic-depressive illness and adds to the growing criticism of that theory."}
{"id": "PMID:472353", "title": "The relationship of 'nuclear' and 'atypical' psychoses: some proposals for a classification of disorders in the borderlands of schizophrenia.", "content": "The paper sets out the arguments in favour of utilising both a 'strict' definition corresponding to the 'nuclear' concept of schizophrenia and a 'broader' definition that also subsumes 'atypical' schizophreniform and related syndromes. Evidence is adduced rejecting the view that attempts to effect a complete separation between 'nuclear' and other forms of schizophrenic disorders based on phenomenological, hereditary, prognostic, etiological and other lines. Both the unity and the diversity of the schizophrenic family of disorders must be given explicit recognition in clinical and scientific work. The value of the 'nuclear' concept has been demonstrated by the IPSS study among other enquiries. The advantages of the broad definition are twofold. It makes for a flexibility and openness of approach in diagnosis and treatment of schizophrenia that are indespensable at the present time. The value of the 'broad' definition is that it encompasses a range of phenomena that have been shown to be related to one another. They may therefore be compared and contrasted. It is argued that new knowledge about the etiology of schizophrenia may be acquired in this manner. Tentative definitions for the 'nuclear' and 'broad' groups of disorder are given.", "contents": "The relationship of 'nuclear' and 'atypical' psychoses: some proposals for a classification of disorders in the borderlands of schizophrenia. The paper sets out the arguments in favour of utilising both a 'strict' definition corresponding to the 'nuclear' concept of schizophrenia and a 'broader' definition that also subsumes 'atypical' schizophreniform and related syndromes. Evidence is adduced rejecting the view that attempts to effect a complete separation between 'nuclear' and other forms of schizophrenic disorders based on phenomenological, hereditary, prognostic, etiological and other lines. Both the unity and the diversity of the schizophrenic family of disorders must be given explicit recognition in clinical and scientific work. The value of the 'nuclear' concept has been demonstrated by the IPSS study among other enquiries. The advantages of the broad definition are twofold. It makes for a flexibility and openness of approach in diagnosis and treatment of schizophrenia that are indespensable at the present time. The value of the 'broad' definition is that it encompasses a range of phenomena that have been shown to be related to one another. They may therefore be compared and contrasted. It is argued that new knowledge about the etiology of schizophrenia may be acquired in this manner. Tentative definitions for the 'nuclear' and 'broad' groups of disorder are given."}
{"id": "PMID:472354", "title": "[Phenomenology and psychodynamics of suicide patients in the awakening phase].", "content": "In the following paper is given a first survey of the phenomenology and psychosomatics of the period of awakening of patients having attempted suicide. The findings of the first period were compared to those elicited one or two days later with the same methods but under different somato-psychic circumsatnces. We found that soon after awakening patients gain some distance from events connected with their suicide. From this observation it is concluded that therapeutic intervention with these patients should begin right during the awakening period.", "contents": "[Phenomenology and psychodynamics of suicide patients in the awakening phase]. In the following paper is given a first survey of the phenomenology and psychosomatics of the period of awakening of patients having attempted suicide. The findings of the first period were compared to those elicited one or two days later with the same methods but under different somato-psychic circumsatnces. We found that soon after awakening patients gain some distance from events connected with their suicide. From this observation it is concluded that therapeutic intervention with these patients should begin right during the awakening period."}
{"id": "PMID:472377", "title": "[Determination of radioactive and stable cobalt in marine biological materials (author's transl)].", "content": "Studies were made to develop the method for rapid determination of radioactive and stable cobalt in a single specimen of marine biological materials. The sample was dried, ashed, and dissolved in acid. The cobalt was extracted with 1-nitroso-2-naphthol benzene and determined by absorptiometry on the benzene phase. Then, the organic solution was evaporated to dryness, and the residue was treated with nitric-perchloric acid mixture to decompose any organic matter, and taken up with hydrochloric acid. The cobolt was extracted from the solution with TIO A-toluene, and the radioactive cobalt was determined by liquid scintillation counting on the toluene phase. Examinations were made on the chemical yield and on the decontamination factor of the fission product nuclides. Analysis were made on the marine biological samples of Urazoko Bay, Fukui prefecture. Agreement of the radioactive cobalt data between that by the present method and that by Ge(Li) gamma ray spectometry was good. Also, the stable cobalt value by the present method agreed well with that by neutron activation analysis method.", "contents": "[Determination of radioactive and stable cobalt in marine biological materials (author's transl)]. Studies were made to develop the method for rapid determination of radioactive and stable cobalt in a single specimen of marine biological materials. The sample was dried, ashed, and dissolved in acid. The cobalt was extracted with 1-nitroso-2-naphthol benzene and determined by absorptiometry on the benzene phase. Then, the organic solution was evaporated to dryness, and the residue was treated with nitric-perchloric acid mixture to decompose any organic matter, and taken up with hydrochloric acid. The cobolt was extracted from the solution with TIO A-toluene, and the radioactive cobalt was determined by liquid scintillation counting on the toluene phase. Examinations were made on the chemical yield and on the decontamination factor of the fission product nuclides. Analysis were made on the marine biological samples of Urazoko Bay, Fukui prefecture. Agreement of the radioactive cobalt data between that by the present method and that by Ge(Li) gamma ray spectometry was good. Also, the stable cobalt value by the present method agreed well with that by neutron activation analysis method."}
{"id": "PMID:472379", "title": "[On renal excretion of sodium 4-iodo-5-methylpyrazole-3-carboxylate-131I (author's transl)].", "content": "Sodium 4-iodo-5-methylpyrazole-3-carboxylate-131I(IMPC-131I) was synthesized by isotope exchange with Na 131I in the molten state to evaluate its potential usefulness as a renal function and/or imaging agent. Tissue distribution and clearance characteristics of IMPC-131I are described. Tissue distribution experiments in rats indicated a very high renal concentration of radioactivity following intravenous administration. At 5 min the kidney took up 27.8% of the injected dose (16.1% dose per gram) and the total activity in the kidney still accounted for 9.8% of the injected dose at 30 min, with only 1.2% localizing in the liver. The ratios for kidney-to-liver in the concentration of IMPC-131I were always higher than those of hippuran -131I throughout the period of the experiment. Within 24 hr, 78.6% of the injected dose were excreted in the urine. The autoradiographic and the scannographic studies were also performed. The present results suggest that IMPC-131I has potential as a new agent for both funciton and imaging studies of the kidney.", "contents": "[On renal excretion of sodium 4-iodo-5-methylpyrazole-3-carboxylate-131I (author's transl)]. Sodium 4-iodo-5-methylpyrazole-3-carboxylate-131I(IMPC-131I) was synthesized by isotope exchange with Na 131I in the molten state to evaluate its potential usefulness as a renal function and/or imaging agent. Tissue distribution and clearance characteristics of IMPC-131I are described. Tissue distribution experiments in rats indicated a very high renal concentration of radioactivity following intravenous administration. At 5 min the kidney took up 27.8% of the injected dose (16.1% dose per gram) and the total activity in the kidney still accounted for 9.8% of the injected dose at 30 min, with only 1.2% localizing in the liver. The ratios for kidney-to-liver in the concentration of IMPC-131I were always higher than those of hippuran -131I throughout the period of the experiment. Within 24 hr, 78.6% of the injected dose were excreted in the urine. The autoradiographic and the scannographic studies were also performed. The present results suggest that IMPC-131I has potential as a new agent for both funciton and imaging studies of the kidney."}
{"id": "PMID:472389", "title": "[Radiologic patterns of the metastatic tumours of the large intestine (author's transl)].", "content": "The metastatic tumours of the large intestine are not unfrequent. In the majority of cases they are due to the direct invasion from endo or retro peritoneal tumours, especially of the stomach and the pancreas. The radiologic findings of such lesions are described, and a correlation is made with the pathologic, pathogenetic and clinical aspects.", "contents": "[Radiologic patterns of the metastatic tumours of the large intestine (author's transl)]. The metastatic tumours of the large intestine are not unfrequent. In the majority of cases they are due to the direct invasion from endo or retro peritoneal tumours, especially of the stomach and the pancreas. The radiologic findings of such lesions are described, and a correlation is made with the pathologic, pathogenetic and clinical aspects."}
{"id": "PMID:472390", "title": "[Lipomatosis of the ileocecal valve (author's transl)].", "content": "A case is described with histologic demonstration. The ethiopathogenetic and clinical aspects of the disease are discussed. The differential diagnosis between lipoma and lipomatosis is stressed. Laparotomy may sometimes be necessary for a correct diagnosis. The basic elements for the treatment are presented.", "contents": "[Lipomatosis of the ileocecal valve (author's transl)]. A case is described with histologic demonstration. The ethiopathogenetic and clinical aspects of the disease are discussed. The differential diagnosis between lipoma and lipomatosis is stressed. Laparotomy may sometimes be necessary for a correct diagnosis. The basic elements for the treatment are presented."}
{"id": "PMID:472391", "title": "[Echotomography in the diagnosis of pancreatic carcinoma. Its reliability in a controlled normal and pathological series of cases].", "content": "Considering the different points of view about the echotomographic findings in pancreatic carcinoma, the authors refer their results about 89 controlled cases (38 normal pancreas and 51 pancreatic carcinomas). The pancreatic space was correctly identified as normal in 37/38 cases. Echotomography also revealed the presence of 48 pancreatic carcinomas; it gave 3 false negative and 3 false positive results; in 21 cases the size of the tumour was 5 cm or less. In a large number of cases echotomography has correctly defined the extent of the lesion by evaluating its size, the secondary liver localization (95% of accuracy) and the vascular involvement (compressions on the inferior vena cava and obstruction of the splenic vein). Basing on the echotomographic findings the authors classify the pancreatic carcinoma in 2 main types.", "contents": "[Echotomography in the diagnosis of pancreatic carcinoma. Its reliability in a controlled normal and pathological series of cases]. Considering the different points of view about the echotomographic findings in pancreatic carcinoma, the authors refer their results about 89 controlled cases (38 normal pancreas and 51 pancreatic carcinomas). The pancreatic space was correctly identified as normal in 37/38 cases. Echotomography also revealed the presence of 48 pancreatic carcinomas; it gave 3 false negative and 3 false positive results; in 21 cases the size of the tumour was 5 cm or less. In a large number of cases echotomography has correctly defined the extent of the lesion by evaluating its size, the secondary liver localization (95% of accuracy) and the vascular involvement (compressions on the inferior vena cava and obstruction of the splenic vein). Basing on the echotomographic findings the authors classify the pancreatic carcinoma in 2 main types."}
{"id": "PMID:472392", "title": "[Multifocal cancer of the breast. Considerations on the mammographic diagnosis].", "content": "A review is carried out of a series of mammographic investigations in 687 tumours observed at the Pascale Institute, Naples, between 1972 and 1977. Mammography shows in a high percentage of cases a multifocality of the disease which was unsuspected. This allows a better evaluation of the stage of the tumour and a correct therapeutic programme.", "contents": "[Multifocal cancer of the breast. Considerations on the mammographic diagnosis]. A review is carried out of a series of mammographic investigations in 687 tumours observed at the Pascale Institute, Naples, between 1972 and 1977. Mammography shows in a high percentage of cases a multifocality of the disease which was unsuspected. This allows a better evaluation of the stage of the tumour and a correct therapeutic programme."}
{"id": "PMID:472393", "title": "[Aortometric correlations in normal and pathological subjects].", "content": "The chest roentgenograms of 691 normal, arteriosclerotic and hypertensive patients have been reviewed for measurements of the aortic knob. There is a significant correlation between the width of the aorta and age, but no such correlation was found with the degree of hypertension. The comparison between average widths of the aorta in the 3 categories of subjects has shown its increase in arteriosclerotic and hypertensive patients.", "contents": "[Aortometric correlations in normal and pathological subjects]. The chest roentgenograms of 691 normal, arteriosclerotic and hypertensive patients have been reviewed for measurements of the aortic knob. There is a significant correlation between the width of the aorta and age, but no such correlation was found with the degree of hypertension. The comparison between average widths of the aorta in the 3 categories of subjects has shown its increase in arteriosclerotic and hypertensive patients."}
{"id": "PMID:472409", "title": "[Organic micropollution of drinking water].", "content": "This work is about organic micropollution of waters sampled at the flowing in and out of three treatment stations. Micropollutants are obtained by chloroformic extraction. It is shown that the extracts have biological effects which can be measured by in vitro and in vivo assays. The treatments reduce the total amount of extractible substances but they induce modifications in their chemical composition and their biological activity. These observations allow to precise how the problem of an eventual health effect of organic micropollution could be approached.", "contents": "[Organic micropollution of drinking water]. This work is about organic micropollution of waters sampled at the flowing in and out of three treatment stations. Micropollutants are obtained by chloroformic extraction. It is shown that the extracts have biological effects which can be measured by in vitro and in vivo assays. The treatments reduce the total amount of extractible substances but they induce modifications in their chemical composition and their biological activity. These observations allow to precise how the problem of an eventual health effect of organic micropollution could be approached."}
{"id": "PMID:472410", "title": "[Epidemic of listeriosis in Western France (1975--1976)].", "content": "Cases of human listeriosis have recently increased in Western France (Maine-et-Loire and Mayenne); until 1974, there were only 15 documented cases yearly where as in 1975, there were 113 cases, and in 1976, 54 documented cases at the hospitals of Angers and Laval. Included amongst the clinical manifestations observed, were 126 cases of materno-fetal infections and 41 cases of either septicemia or meningo-encephalitis. The bacteriological study revealed a certain variability of biological characteristics examined, the overwhelming frequence of bacteria of the strain 4 b and the predictable sensitivity of this strain to antibiotics commonly employed in cases of listeriosis. The human cases were notably more frequent between January and June but their geographical distribution was not related to cases of either animal disease nor listeria that was isolated from corn silage. In this report, we propose an etio-pathological explanation for this epidemic.", "contents": "[Epidemic of listeriosis in Western France (1975--1976)]. Cases of human listeriosis have recently increased in Western France (Maine-et-Loire and Mayenne); until 1974, there were only 15 documented cases yearly where as in 1975, there were 113 cases, and in 1976, 54 documented cases at the hospitals of Angers and Laval. Included amongst the clinical manifestations observed, were 126 cases of materno-fetal infections and 41 cases of either septicemia or meningo-encephalitis. The bacteriological study revealed a certain variability of biological characteristics examined, the overwhelming frequence of bacteria of the strain 4 b and the predictable sensitivity of this strain to antibiotics commonly employed in cases of listeriosis. The human cases were notably more frequent between January and June but their geographical distribution was not related to cases of either animal disease nor listeria that was isolated from corn silage. In this report, we propose an etio-pathological explanation for this epidemic."}
{"id": "PMID:472471", "title": "[Histological and histoautoradiographical examination of the interposed jejunum after total gastrectomy in dogs (author's transl)].", "content": "Total gastrectomy was performed on purebred beagle dogs and a 30--40 cm jejunal segment was grafted isoperistaltically between oesophagus and duodenum. Histological and histoautoradiographical examinations were carried out and the wall-thickness and the cell-transformation rate in the interponate determined. Despite the isoperistaltic substitute-stomach a muscular wall-hypertrophy of 140% of the interponated jejunum was found. The reason for this hypertrophy is thought to be the result of adhesions and the functional mechanical demands. Histoautoradiographical examinations confirmed that the regeneration time in the substitute-stomach decreased by 70% compared with the normal jejunum. The distinct reduction of cell regeneration in the regeneration in the interponated jejunum could be attributed to the considerable regression of the absorbent performance and to the increase in its mechanical exercises.", "contents": "[Histological and histoautoradiographical examination of the interposed jejunum after total gastrectomy in dogs (author's transl)]. Total gastrectomy was performed on purebred beagle dogs and a 30--40 cm jejunal segment was grafted isoperistaltically between oesophagus and duodenum. Histological and histoautoradiographical examinations were carried out and the wall-thickness and the cell-transformation rate in the interponate determined. Despite the isoperistaltic substitute-stomach a muscular wall-hypertrophy of 140% of the interponated jejunum was found. The reason for this hypertrophy is thought to be the result of adhesions and the functional mechanical demands. Histoautoradiographical examinations confirmed that the regeneration time in the substitute-stomach decreased by 70% compared with the normal jejunum. The distinct reduction of cell regeneration in the regeneration in the interponated jejunum could be attributed to the considerable regression of the absorbent performance and to the increase in its mechanical exercises."}
{"id": "PMID:472472", "title": "The effects of intra-arterially administered histamine on vascular permeability.", "content": "The influence of intra-arterially administered histamine on the permeability of the articular barrier (synovial membrane plus vascular endothelium) was studied in cats. The dye penetration from the blood through the barrier was tested by perfusing the two knee joints with saline; the histamine solution was infused through one of the circumflex branches of the femoral artery. The dye content of the two perfusates was measured colorimetrically, the local blood supply of the two knee joints thermo-electrically. In the great majority of experiments it was found that the dye excretion was considerably reduced on the histamine-infused side, in spite of marked improvement of blood supply.", "contents": "The effects of intra-arterially administered histamine on vascular permeability. The influence of intra-arterially administered histamine on the permeability of the articular barrier (synovial membrane plus vascular endothelium) was studied in cats. The dye penetration from the blood through the barrier was tested by perfusing the two knee joints with saline; the histamine solution was infused through one of the circumflex branches of the femoral artery. The dye content of the two perfusates was measured colorimetrically, the local blood supply of the two knee joints thermo-electrically. In the great majority of experiments it was found that the dye excretion was considerably reduced on the histamine-infused side, in spite of marked improvement of blood supply."}
{"id": "PMID:472473", "title": "The influence of linoleic acid intake on the excretion of urinary prostaglandin metabolites.", "content": "The influence of linoleic acid intake on human prostaglandin formation was investigated by gas chromatographic determination of dimethyl tetranorprostanedioate, a derivative of the major urinary end products of prostaglandin metabolism. Six healthy female volunteers were put on liquid formula diets. Each person had a linoleic acid supply of 0, 10, and 50 g per day for periods of two weeks. At the end of these periods the corresponding average amounts of tetranorprostanedioic acid in urine were 92, 175, and 326 microgram per day.", "contents": "The influence of linoleic acid intake on the excretion of urinary prostaglandin metabolites. The influence of linoleic acid intake on human prostaglandin formation was investigated by gas chromatographic determination of dimethyl tetranorprostanedioate, a derivative of the major urinary end products of prostaglandin metabolism. Six healthy female volunteers were put on liquid formula diets. Each person had a linoleic acid supply of 0, 10, and 50 g per day for periods of two weeks. At the end of these periods the corresponding average amounts of tetranorprostanedioic acid in urine were 92, 175, and 326 microgram per day."}
{"id": "PMID:472474", "title": "Investigation of a glycoprotein-protein mixture from urine of anemic patients by analytical isotachophoresis.", "content": "Nine glycoprotein-protein fractions prepared of erythropoietin-active crude urinary protein and separated by gel filtration on Sephadex G-50, ion exchange chromatography on Sephadex DEAE A-50, gel filtration on Sephacryl S-200 and Sepharose 6B were investigated by analytical isotachophoresis. 7 to 38 components could be separated of every chromatographic fraction investigated using chloride as leading and glycinate as terminating electrolyte. Improvement of resolution is possible by variation of buffer system conditions.", "contents": "Investigation of a glycoprotein-protein mixture from urine of anemic patients by analytical isotachophoresis. Nine glycoprotein-protein fractions prepared of erythropoietin-active crude urinary protein and separated by gel filtration on Sephadex G-50, ion exchange chromatography on Sephadex DEAE A-50, gel filtration on Sephacryl S-200 and Sepharose 6B were investigated by analytical isotachophoresis. 7 to 38 components could be separated of every chromatographic fraction investigated using chloride as leading and glycinate as terminating electrolyte. Improvement of resolution is possible by variation of buffer system conditions."}
{"id": "PMID:472475", "title": "Hypocalcemia during restraint stress in rats. Indication that gastric ulcer prophylaxis by exogenous calcium interferes with calcitonin release.", "content": "The influence of restraint stress on serum calcium (Ca) and phosphate was studied in normal and thyroidectomized rats. In addition the response of gastric stress ulcer index, blood gastrin and glucagon to exogenous Ca was investigated. In intact as well as in thyroidectomized animals serum total, ionised and previously injected radioactive Ca decrease during an 8h stress period, whereas inorganic phosphate increases. Together with a constant specific activity these findings are consistent with hypoparathyroidism and calcitonin independent hypocalcemia during stress. Intragastric infusion of 45 mg/kg Ca-gluconate per 8h proves to be a potent anti-stress ulcer regimen in intact and neck-sham operated, but not in thyroidectomized rats without and with additional adrenal demedullation. Gastrin and glucagon were not correlated with calcemia during either stress alone or stress combined with intragastric Ca infusion. It is suggested that the development of gastric stress ulcerations can be prevented by a Ca-mediated release of endogenous calcitonin.", "contents": "Hypocalcemia during restraint stress in rats. Indication that gastric ulcer prophylaxis by exogenous calcium interferes with calcitonin release. The influence of restraint stress on serum calcium (Ca) and phosphate was studied in normal and thyroidectomized rats. In addition the response of gastric stress ulcer index, blood gastrin and glucagon to exogenous Ca was investigated. In intact as well as in thyroidectomized animals serum total, ionised and previously injected radioactive Ca decrease during an 8h stress period, whereas inorganic phosphate increases. Together with a constant specific activity these findings are consistent with hypoparathyroidism and calcitonin independent hypocalcemia during stress. Intragastric infusion of 45 mg/kg Ca-gluconate per 8h proves to be a potent anti-stress ulcer regimen in intact and neck-sham operated, but not in thyroidectomized rats without and with additional adrenal demedullation. Gastrin and glucagon were not correlated with calcemia during either stress alone or stress combined with intragastric Ca infusion. It is suggested that the development of gastric stress ulcerations can be prevented by a Ca-mediated release of endogenous calcitonin."}
{"id": "PMID:472476", "title": "Regional gastric mucosal blood flow in hypovolemic shock.", "content": "The effect of hypovolemia on the mucosal blood flow of the gastric corpus and antrum were tested with the microsphere method 30 and 60 min after induction of hemorrhagic shock in the rat. The results demonstrate a marked reduction after 30 and 60 min without any significant differences between the corpus and the antrum. This reduction is largely dependent on cardiac and circulatory function. It is concluded that the differences in energy metabolism, which have been implicated as explanation for the different susceptibility to develop stress lesions by Menguy and Masters, cannot be attributed tod different degrees of ischemia.", "contents": "Regional gastric mucosal blood flow in hypovolemic shock. The effect of hypovolemia on the mucosal blood flow of the gastric corpus and antrum were tested with the microsphere method 30 and 60 min after induction of hemorrhagic shock in the rat. The results demonstrate a marked reduction after 30 and 60 min without any significant differences between the corpus and the antrum. This reduction is largely dependent on cardiac and circulatory function. It is concluded that the differences in energy metabolism, which have been implicated as explanation for the different susceptibility to develop stress lesions by Menguy and Masters, cannot be attributed tod different degrees of ischemia."}
{"id": "PMID:472477", "title": "The effect of the duration and type of stress on some serum enzyme levels in pigs.", "content": "The effects of blood sampling from the ear vein, jugular vein and collection on exsanguination, on serum CK, LDH, GOT and PK activities were investigated. The activities of LDH and PK were higher in ear vein vena cava samples. The effect of pre-slaughter handling was also investigated. In general there was a significant progressive increase in serum enzyme activities from farm to slaughter, although the magnitude of the change varied for different enzymes.", "contents": "The effect of the duration and type of stress on some serum enzyme levels in pigs. The effects of blood sampling from the ear vein, jugular vein and collection on exsanguination, on serum CK, LDH, GOT and PK activities were investigated. The activities of LDH and PK were higher in ear vein vena cava samples. The effect of pre-slaughter handling was also investigated. In general there was a significant progressive increase in serum enzyme activities from farm to slaughter, although the magnitude of the change varied for different enzymes."}
{"id": "PMID:472478", "title": "Inhibited development of trichostrongylids of sheep in northern Nigeria.", "content": "Worm counts from Yankassa ewes, lambs and tracer lambs grazing unimproved pasture at Zaria were obtained each month during the rainy season of 1976. In June and July up to 14 per cent of the Haemonchus contortus populations consisted of early fourth stage larvae (L4). In August this increased to more than 55 per cent and in October to more than 95 per cent. From June to August most of the abundant late L4 contained rodlike crystals. More than 95 per cent of the Haemonchus contortus populations of two lambs killed two weeks after infection with infective larvae, cultured for seven days at 25 degrees to 30 degrees C in the dark, were early L4. Inhibited 3rd stage Trichostrongylus spp did not exceed 26 per cent of the worm populations.", "contents": "Inhibited development of trichostrongylids of sheep in northern Nigeria. Worm counts from Yankassa ewes, lambs and tracer lambs grazing unimproved pasture at Zaria were obtained each month during the rainy season of 1976. In June and July up to 14 per cent of the Haemonchus contortus populations consisted of early fourth stage larvae (L4). In August this increased to more than 55 per cent and in October to more than 95 per cent. From June to August most of the abundant late L4 contained rodlike crystals. More than 95 per cent of the Haemonchus contortus populations of two lambs killed two weeks after infection with infective larvae, cultured for seven days at 25 degrees to 30 degrees C in the dark, were early L4. Inhibited 3rd stage Trichostrongylus spp did not exceed 26 per cent of the worm populations."}
{"id": "PMID:472480", "title": "The possible fate of crystal harbouring larvae of Haemonchus contortus.", "content": "An examination of the occurrence of crystalline inclusions in the intestinal cells of fourth and fifth stage Haemonchus contortus revealed that a high percentage of inhibited early fourth stage larvae (L4) and developing L4 and a lower percentage of early fifth stage larvae (L5) harboured these crystals. Before the fourth moult crystals were falling into pieces, indicating that worms harbouring crystals can develop further. The presence of crystals may be associated with a retardation of development.", "contents": "The possible fate of crystal harbouring larvae of Haemonchus contortus. An examination of the occurrence of crystalline inclusions in the intestinal cells of fourth and fifth stage Haemonchus contortus revealed that a high percentage of inhibited early fourth stage larvae (L4) and developing L4 and a lower percentage of early fifth stage larvae (L5) harboured these crystals. Before the fourth moult crystals were falling into pieces, indicating that worms harbouring crystals can develop further. The presence of crystals may be associated with a retardation of development."}
{"id": "PMID:472481", "title": "The role of maternal triglycerides in the supply of lipids to the ovine fetus.", "content": "Maternal and fetal blood samples were obtained for up to 20 min after the injection of an emulsion of 14C-labelled glycerol tripalmitate into the circulation of pregnant ewes. No evidence was obtained for the direct transfer of intact triglycerides across the placenta. Results obtained suggest that there was a very rapid uptake of the triglyceride from the maternal circulation followed by hydrolysis and release of free fatty acids back into the maternal circulation.", "contents": "The role of maternal triglycerides in the supply of lipids to the ovine fetus. Maternal and fetal blood samples were obtained for up to 20 min after the injection of an emulsion of 14C-labelled glycerol tripalmitate into the circulation of pregnant ewes. No evidence was obtained for the direct transfer of intact triglycerides across the placenta. Results obtained suggest that there was a very rapid uptake of the triglyceride from the maternal circulation followed by hydrolysis and release of free fatty acids back into the maternal circulation."}
{"id": "PMID:472482", "title": "The effect of triamcinolone acetonide on plasma glucose and ketone concentration and on the total entry rate of glucose in twin pregnant hypoglycaemic ketotic sheep.", "content": "Hypoglycaemic ketosis clinically resembling pregnancy toxaemia was induced by starvation in Clun Forest sheep pregnant with twins. Total glucose entry was measured by the continuous infusion of trace amounts of (2-3H) glucose before and after the induction of ketosis and 24 h after the intramuscular injection of the ketotic sheep with triamcinolone acetonide (0.5 mg/kg). The concentration of glucose in plasma fell significantly after the induction of ketosis and rose significantly after the injection of steroid. The total entry rate of glucose fell significantly after the induction of ketosis and rose, but no significantly, after steroid injection. It is suggested that the dose of steroid which was used may inhibit glucose utilisation by peripheral tissues. The induction of ketosis significantly reduced the duration of pregnancy but the steroid injection did not produce a significant additional effect.", "contents": "The effect of triamcinolone acetonide on plasma glucose and ketone concentration and on the total entry rate of glucose in twin pregnant hypoglycaemic ketotic sheep. Hypoglycaemic ketosis clinically resembling pregnancy toxaemia was induced by starvation in Clun Forest sheep pregnant with twins. Total glucose entry was measured by the continuous infusion of trace amounts of (2-3H) glucose before and after the induction of ketosis and 24 h after the intramuscular injection of the ketotic sheep with triamcinolone acetonide (0.5 mg/kg). The concentration of glucose in plasma fell significantly after the induction of ketosis and rose significantly after the injection of steroid. The total entry rate of glucose fell significantly after the induction of ketosis and rose, but no significantly, after steroid injection. It is suggested that the dose of steroid which was used may inhibit glucose utilisation by peripheral tissues. The induction of ketosis significantly reduced the duration of pregnancy but the steroid injection did not produce a significant additional effect."}
{"id": "PMID:472483", "title": "The rate of recovery of intestinal morphology following anthelmintic treatment of parasitised sheep.", "content": "Sixteen sheep which had previously been infected with Ostertagia circumcincta were dosed with 4000 Trichostrongylus colubriformis larvae/day for four weeks, then given anthelmintic and killed at intervals thereafter. Histological examination of the small intestine showed that two to three weeks were required for restoration of a normal mucosal architecture.", "contents": "The rate of recovery of intestinal morphology following anthelmintic treatment of parasitised sheep. Sixteen sheep which had previously been infected with Ostertagia circumcincta were dosed with 4000 Trichostrongylus colubriformis larvae/day for four weeks, then given anthelmintic and killed at intervals thereafter. Histological examination of the small intestine showed that two to three weeks were required for restoration of a normal mucosal architecture."}
{"id": "PMID:472484", "title": "Clinical and anatomical studies on the scrotal and pelvic genitalia of Sokoto Gudali bulls in Northern Nigeria.", "content": "The scrotal and pelvic genitalia from 2400 Sokoto Gudali bulls aged three to five years were studied clinically (by ante mortem palpation and measurement) and anatomically (by direct examination and measurement of the post mortem material). In general the difference between the mean clinical and anatomical measurements was not significant (at the 5 per cent level). Gross abnormalities observed included: testicular degeneration, 60 bulls (2.5 per cent); orchitis, 48 bulls (2.0 per cent); testicular hypoplasia, 35 bulls (1.45 per cent); epididymitis, 37 bulls (1.54 per cent); spermatic granuloma, two bulls (0.08 per cent); seminovesiculitis, 43 bulls (1.79 per cent); scrotal stryptortrichosis, 126 bulls (5.25 per cent). Lesions had no seasonal pattern and occurred randomly throughout the year.", "contents": "Clinical and anatomical studies on the scrotal and pelvic genitalia of Sokoto Gudali bulls in Northern Nigeria. The scrotal and pelvic genitalia from 2400 Sokoto Gudali bulls aged three to five years were studied clinically (by ante mortem palpation and measurement) and anatomically (by direct examination and measurement of the post mortem material). In general the difference between the mean clinical and anatomical measurements was not significant (at the 5 per cent level). Gross abnormalities observed included: testicular degeneration, 60 bulls (2.5 per cent); orchitis, 48 bulls (2.0 per cent); testicular hypoplasia, 35 bulls (1.45 per cent); epididymitis, 37 bulls (1.54 per cent); spermatic granuloma, two bulls (0.08 per cent); seminovesiculitis, 43 bulls (1.79 per cent); scrotal stryptortrichosis, 126 bulls (5.25 per cent). Lesions had no seasonal pattern and occurred randomly throughout the year."}
{"id": "PMID:472485", "title": "Concentrations of immunoglobulin classes in subcompartments of the sheep lung.", "content": "The concentrations of IgA, IgG and IgM were measured in plasma, lung lymph and lung lavage fluid from adult sheep. Immunoglobulin concentrations in lymph and lavage fluid should fall sequentially during diffusive flux from the vascular compartment as a result of molecular sieving at the capillary endothelium and the alveolar epithelium. This was seen only for the IgG class. High concentrations of IgA and IgM in the interstitial (lymph) and luminal (lavage) compartments of the lung (relative to plasma levels) suggest either local synthesis of these classes or the existence of class specific active transport.", "contents": "Concentrations of immunoglobulin classes in subcompartments of the sheep lung. The concentrations of IgA, IgG and IgM were measured in plasma, lung lymph and lung lavage fluid from adult sheep. Immunoglobulin concentrations in lymph and lavage fluid should fall sequentially during diffusive flux from the vascular compartment as a result of molecular sieving at the capillary endothelium and the alveolar epithelium. This was seen only for the IgG class. High concentrations of IgA and IgM in the interstitial (lymph) and luminal (lavage) compartments of the lung (relative to plasma levels) suggest either local synthesis of these classes or the existence of class specific active transport."}
{"id": "PMID:472486", "title": "Characterisation of influenza viruses isolated from turkeys in Great Britain during 1963--1977.", "content": "Seven influenza viruses isolated from turkeys in Great Britain since 1963 were typed by haemagglutination inhibition and neuraminidase inhibition tests as: A/turkey/England/63 (Hav 1 Nav 3), A/turkey/England/66 (Hav 6 N2), A/turkey/England/69 (Hav 7 N2), A/turkey/Scotland/70 (Hav ? Neq 1), A/turkey/England/N28/73 (Hav 5 N2), A/turkey/England/110/77 (Hav 6 N2), A/turkey/England/647/77 /Hav 1 Neq 1). A/turkey/Scotland/70 failed to show a haemagglutinin relationship with any of the representative strains and may possess a hitherto unreported haemagglutinin subtype. Intravenous pathogenicity tests in six-week-old birds showed only A/turkey/England/63 to have high virulence for turkeys and chickens. A/turkey/England/69 produced some signs of disease in chickens and, to a lesser extent, turkeys but all other isolates were avirulent.", "contents": "Characterisation of influenza viruses isolated from turkeys in Great Britain during 1963--1977. Seven influenza viruses isolated from turkeys in Great Britain since 1963 were typed by haemagglutination inhibition and neuraminidase inhibition tests as: A/turkey/England/63 (Hav 1 Nav 3), A/turkey/England/66 (Hav 6 N2), A/turkey/England/69 (Hav 7 N2), A/turkey/Scotland/70 (Hav ? Neq 1), A/turkey/England/N28/73 (Hav 5 N2), A/turkey/England/110/77 (Hav 6 N2), A/turkey/England/647/77 /Hav 1 Neq 1). A/turkey/Scotland/70 failed to show a haemagglutinin relationship with any of the representative strains and may possess a hitherto unreported haemagglutinin subtype. Intravenous pathogenicity tests in six-week-old birds showed only A/turkey/England/63 to have high virulence for turkeys and chickens. A/turkey/England/69 produced some signs of disease in chickens and, to a lesser extent, turkeys but all other isolates were avirulent."}
{"id": "PMID:472487", "title": "Pasteurella multocida antigen-induced in vitro lymphocyte immunostimulation, using whole blood from cattle and turkeys.", "content": "A whole blood lymphocyte stimulation assay to study cell-mediated immune responses in bovine pasteurellosis was developed. Peripheral blood lymphocytes from cattle artificially immunised with three Asiatic haemorrhagic septicaemia strains of Pasteurella multocida exhibited higher stimulation indices when incubated with antigen preparations from homologous strains than with the heterologous shipping fever strain. Lymphocytes from cattle immunised with the shipping fever strain of P multocida exhibited a higher stimulation index when incubated with an antigen preparation from the homolgous strain than with antigen preparations from heterologous haemorrhagic septicaemia strains. These results suggest that immunogenic differences exist between the haemorrhagic septicaemia strains and the shipping fever strain of P multocida. An assay using turkey whole blood lymphocytes was also developed. The use of small amounts of whole blood, microtitre plates, either 125I iododeoxyuridine or 3H-thymidine as the labelling agent, and a multiple cell-culture harvester makes the method simple, rapid and suitable for the study of immune competence and cell-mediated immune responses in turkeys on a flock basis.", "contents": "Pasteurella multocida antigen-induced in vitro lymphocyte immunostimulation, using whole blood from cattle and turkeys. A whole blood lymphocyte stimulation assay to study cell-mediated immune responses in bovine pasteurellosis was developed. Peripheral blood lymphocytes from cattle artificially immunised with three Asiatic haemorrhagic septicaemia strains of Pasteurella multocida exhibited higher stimulation indices when incubated with antigen preparations from homologous strains than with the heterologous shipping fever strain. Lymphocytes from cattle immunised with the shipping fever strain of P multocida exhibited a higher stimulation index when incubated with an antigen preparation from the homolgous strain than with antigen preparations from heterologous haemorrhagic septicaemia strains. These results suggest that immunogenic differences exist between the haemorrhagic septicaemia strains and the shipping fever strain of P multocida. An assay using turkey whole blood lymphocytes was also developed. The use of small amounts of whole blood, microtitre plates, either 125I iododeoxyuridine or 3H-thymidine as the labelling agent, and a multiple cell-culture harvester makes the method simple, rapid and suitable for the study of immune competence and cell-mediated immune responses in turkeys on a flock basis."}
{"id": "PMID:472488", "title": "Investigations of 'transfer factor' activity in the transfer of immunity to Trichostrongylus axei infections in sheep.", "content": "Three investigations are described in which non-dialysed and dialysed leucocyte lysates, 'transfer factor' prepared from the blood of sheep infected with Trichostrongylus axei successfully transferred immunity to challenge infection with that parasite in susceptible lambs. Similar leucocyte lysates from parasite-free lambs failed to transfer a similar resistance to challenge infection. 'Transfer factor' treatment produced a 30--72 per cent reduction in a total worm burden compared to susceptible control lambs. In the first two investigations the donor and recipient lambs were genetically dissimilar and in the third investigation were of different breeds. The resistance transfer was considered to operate independently of immune incompetence.", "contents": "Investigations of 'transfer factor' activity in the transfer of immunity to Trichostrongylus axei infections in sheep. Three investigations are described in which non-dialysed and dialysed leucocyte lysates, 'transfer factor' prepared from the blood of sheep infected with Trichostrongylus axei successfully transferred immunity to challenge infection with that parasite in susceptible lambs. Similar leucocyte lysates from parasite-free lambs failed to transfer a similar resistance to challenge infection. 'Transfer factor' treatment produced a 30--72 per cent reduction in a total worm burden compared to susceptible control lambs. In the first two investigations the donor and recipient lambs were genetically dissimilar and in the third investigation were of different breeds. The resistance transfer was considered to operate independently of immune incompetence."}
{"id": "PMID:472489", "title": "Effects of dietary protein and stage of lactation on the haematology and erythrocyte enzymes activities of high-producing dairy cattle.", "content": "Blood samples were collected monthly over a nine-month period from 19 high-producing Holstein-Friesian dairy cows. Dry cows on the lowest (13 per cent) protein ration had the highest mean values for packed cell volume (PCV), haemoglobin (Hb) and red blood cells (RBC). Among the lactating cows, the group on the 13 per cent protein diet had the highest mean PCV, Hb and RBC values. Other constituents were not affected significantly by dietary protein levels. Packed cell volume, RBC, Hb, serum iron (SI), iron binding capacity (IBC) and serum albumin concentrations decreased early in lactation and rose to pre-lactation levels by mid-lactation. PCV and Hb concentrations remained low for periods up to four months. RBC count was lowest in the second month while albumin concentration was lowest in the first month and remained low up to the second month. IBC was lowest in the first month of lactation while SI concentrations were lowest in the third month. There were no significant variations in the activities of erythrocyte glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD) and reduced glutathione (GSH). The 13 per cent protein ration had no anaemia-inducing effect on the cows.", "contents": "Effects of dietary protein and stage of lactation on the haematology and erythrocyte enzymes activities of high-producing dairy cattle. Blood samples were collected monthly over a nine-month period from 19 high-producing Holstein-Friesian dairy cows. Dry cows on the lowest (13 per cent) protein ration had the highest mean values for packed cell volume (PCV), haemoglobin (Hb) and red blood cells (RBC). Among the lactating cows, the group on the 13 per cent protein diet had the highest mean PCV, Hb and RBC values. Other constituents were not affected significantly by dietary protein levels. Packed cell volume, RBC, Hb, serum iron (SI), iron binding capacity (IBC) and serum albumin concentrations decreased early in lactation and rose to pre-lactation levels by mid-lactation. PCV and Hb concentrations remained low for periods up to four months. RBC count was lowest in the second month while albumin concentration was lowest in the first month and remained low up to the second month. IBC was lowest in the first month of lactation while SI concentrations were lowest in the third month. There were no significant variations in the activities of erythrocyte glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD) and reduced glutathione (GSH). The 13 per cent protein ration had no anaemia-inducing effect on the cows."}
{"id": "PMID:472490", "title": "The experimental infection of calves with a British strain of Haemophilus somnus.", "content": "Intravenous inoculation of a British strain of Haemophilus somnus resulted either in fatal peracute or chronic forms of H somnus disease. Pleuritis, pericarditis and arthritis were prominent post mortem findings. Intratracheal inoculation led to severe cellulitis and abscess formation in the neck with acute suppurative bronchopneumonia in one calf, while in another, encapsulated abscesses and pleural adhesions were seen. Intraperitoneal inoculation resulted in fatal fibrinous peritonitis in one calf. The organism was readily recovered from the lesions except when overgrown by post mortem contaminants. Rising antibody titres were detected in two calves 12 days after inoculation by a micro-complement fixation test, and the organism was demonstrated in synovial fluid by an indirect fluorescent antibody test. No organisms were recovered from and no lesions were seen in the nervous tissues of the calves in contrast to studies of this disease in the USA.", "contents": "The experimental infection of calves with a British strain of Haemophilus somnus. Intravenous inoculation of a British strain of Haemophilus somnus resulted either in fatal peracute or chronic forms of H somnus disease. Pleuritis, pericarditis and arthritis were prominent post mortem findings. Intratracheal inoculation led to severe cellulitis and abscess formation in the neck with acute suppurative bronchopneumonia in one calf, while in another, encapsulated abscesses and pleural adhesions were seen. Intraperitoneal inoculation resulted in fatal fibrinous peritonitis in one calf. The organism was readily recovered from the lesions except when overgrown by post mortem contaminants. Rising antibody titres were detected in two calves 12 days after inoculation by a micro-complement fixation test, and the organism was demonstrated in synovial fluid by an indirect fluorescent antibody test. No organisms were recovered from and no lesions were seen in the nervous tissues of the calves in contrast to studies of this disease in the USA."}
{"id": "PMID:472491", "title": "The density of equine tissue at 37 degrees C.", "content": "The density of the following equine tissues was measured: white and grey brain matter, myocardium, lung parenchyma, liver, spleen, gastrointestinal tract and contents, renal medulla and cortex, muscle, fat, tendon and bone. Statistical analysis indicated that there were highly significant differences in the values for density between horses and between tissues within horses. Values for density of eight different muscles were obtained for 18 horses and these were shown to differ highly significantly between horses and between muscles within horses. The values for splenic density differed highly significantly between those for knackery killed horses and for the barbiturate killed post morten room horses.", "contents": "The density of equine tissue at 37 degrees C. The density of the following equine tissues was measured: white and grey brain matter, myocardium, lung parenchyma, liver, spleen, gastrointestinal tract and contents, renal medulla and cortex, muscle, fat, tendon and bone. Statistical analysis indicated that there were highly significant differences in the values for density between horses and between tissues within horses. Values for density of eight different muscles were obtained for 18 horses and these were shown to differ highly significantly between horses and between muscles within horses. The values for splenic density differed highly significantly between those for knackery killed horses and for the barbiturate killed post morten room horses."}
{"id": "PMID:472492", "title": "Changes in serum immunoglobulin levels in Jerseys and Friesians near calving.", "content": "IgG1 and IgM levels were significantly lower in bovine serum from four weeks before, until at least two months after calving, whereas IgG2 levels were elevated at this time. The shapes of the changes in Ig levels were different for first and second calving and it appeared that IgG1 levels were secreted earlier, from serum to colostrum, in heifers. Changes in management caused marked changes in Ig levels particularly after third calving when the cows were transferred from a balanced complete diet to grass or hay. IgG1 levels increased by 130 per cent and stayed high whereas the increase in IgM was transitory, and IgG2 levels largely unaffected. In Friesians the IgG2 levels increased with age as anticipated from previous studies but in Jerseys the levels appeared to decline with age. Mean IgG2 levels in Jerseys were also significantly lower than in Friesians.", "contents": "Changes in serum immunoglobulin levels in Jerseys and Friesians near calving. IgG1 and IgM levels were significantly lower in bovine serum from four weeks before, until at least two months after calving, whereas IgG2 levels were elevated at this time. The shapes of the changes in Ig levels were different for first and second calving and it appeared that IgG1 levels were secreted earlier, from serum to colostrum, in heifers. Changes in management caused marked changes in Ig levels particularly after third calving when the cows were transferred from a balanced complete diet to grass or hay. IgG1 levels increased by 130 per cent and stayed high whereas the increase in IgM was transitory, and IgG2 levels largely unaffected. In Friesians the IgG2 levels increased with age as anticipated from previous studies but in Jerseys the levels appeared to decline with age. Mean IgG2 levels in Jerseys were also significantly lower than in Friesians."}
{"id": "PMID:472493", "title": "The effect of single and divided dose administration on the efficacy of fenbendazole against adult stages of benzimidazole resistant sheep trichostrongylids.", "content": "Sheep infected with benzimidazole resistant strains of Haemonchus contortus and Trichostrongylus colubriformis were used to compare the anthelmintic efficacy of fenbendazole given as a single dose or administered in a divided dose regime over five days. Statistical analysis showed no significant difference between the two methods of administration for H contortus. On the other hand, divided dose fenbendazole was significantly less effective than single doses against adult T colubriformis at dose rates of 5 and 7.5 mg/kg. In the case of H contortus a highly significant correlation coefficient between post treatment egg counts and worm counts (r = 0.789) was obtained. This suggests that reduction in faecal egg output following drug treatment would provide a useful field indication of anthelmintic performance of fenbendazole (and possibly related compounds) against benzimidazole resistant strains of this parasite.", "contents": "The effect of single and divided dose administration on the efficacy of fenbendazole against adult stages of benzimidazole resistant sheep trichostrongylids. Sheep infected with benzimidazole resistant strains of Haemonchus contortus and Trichostrongylus colubriformis were used to compare the anthelmintic efficacy of fenbendazole given as a single dose or administered in a divided dose regime over five days. Statistical analysis showed no significant difference between the two methods of administration for H contortus. On the other hand, divided dose fenbendazole was significantly less effective than single doses against adult T colubriformis at dose rates of 5 and 7.5 mg/kg. In the case of H contortus a highly significant correlation coefficient between post treatment egg counts and worm counts (r = 0.789) was obtained. This suggests that reduction in faecal egg output following drug treatment would provide a useful field indication of anthelmintic performance of fenbendazole (and possibly related compounds) against benzimidazole resistant strains of this parasite."}
{"id": "PMID:472494", "title": "The anthelmintic efficacy of albendazole against Fasciola hepatica and benzimidazole resistant strains of Haemonchus contortus and Trichostrongylus colubriformis in sheep.", "content": "The anthelmintic efficacy of albendazole (methyl [5-(propylthio) - 1H - benzimidazole -2 -yl] carbamate) against immature and adult Fasciola hepatica and against standardised strains of benzimidazole resistant Haemonchus contortus and Trichostrongylus colubriformis was evaluated in experimentally infected sheep. A single intrarumenal treatment of dose rates of 3.8 and 7.6 mg/kg was ineffective against immature (six weeks old) F hepatica. Dose rates of 5.7 and 7.6 mg/kg reduced the number of mature (12 weeks old) F hepatica by 70 and 91 per cent respectively. Dose rates of 5.7 and 7.6 mg/kg removed 92 and 99 per cent of four-week-old, benzimidazole resistant H contortus and 89 and 99 per cent of four-week-old, benzimidazole resistant T colubriformis.", "contents": "The anthelmintic efficacy of albendazole against Fasciola hepatica and benzimidazole resistant strains of Haemonchus contortus and Trichostrongylus colubriformis in sheep. The anthelmintic efficacy of albendazole (methyl [5-(propylthio) - 1H - benzimidazole -2 -yl] carbamate) against immature and adult Fasciola hepatica and against standardised strains of benzimidazole resistant Haemonchus contortus and Trichostrongylus colubriformis was evaluated in experimentally infected sheep. A single intrarumenal treatment of dose rates of 3.8 and 7.6 mg/kg was ineffective against immature (six weeks old) F hepatica. Dose rates of 5.7 and 7.6 mg/kg reduced the number of mature (12 weeks old) F hepatica by 70 and 91 per cent respectively. Dose rates of 5.7 and 7.6 mg/kg removed 92 and 99 per cent of four-week-old, benzimidazole resistant H contortus and 89 and 99 per cent of four-week-old, benzimidazole resistant T colubriformis."}
{"id": "PMID:472495", "title": "The isolation of peste des petits ruminants virus from Nigerian sheep and goats.", "content": "Four isolates of peste des petits ruminants virus were obtained from sick Nigerian sheep and goats. One was identical antigenically with the prototype Senegalese strain. A cross relationship was found between peste des petits ruminants virus and rinderpest virus based on neutralisation in vitro.", "contents": "The isolation of peste des petits ruminants virus from Nigerian sheep and goats. Four isolates of peste des petits ruminants virus were obtained from sick Nigerian sheep and goats. One was identical antigenically with the prototype Senegalese strain. A cross relationship was found between peste des petits ruminants virus and rinderpest virus based on neutralisation in vitro."}
{"id": "PMID:472497", "title": "Dco at various breath-holding times: comparison in patients with chronic bronchial asthma and emphysema.", "content": "Measurement of Dco is known to be dependent upon functional inhomogeneities. Because different types of inhomogeneities are operative in patients with bronchial asthma and patients with emphysema, different changes of Dco with increasing breath-holding time, tA, are to be expected. We studied the change of Dco with increasing breath-holding time in healthy subjects, patients with asthma bronchiale and patients with emphysema. In the patients the severity of airway obstruction was about the same. The following results were obtained: (a) in healthy subjects and in the asthmatics Dco decreased with tA, in a similar manner, approaching a value (ml . min-1 . Terror-1) of 34.7 and 31.6 at 10 sec, respectively, and (b) in patients with emphysema Dco increased with tA, yielding negative values at small tA: 1.5 sec-23.4; 10 sec: 11.7. From these results we suggest that in healthy subjects and in patients with bronchial asthma parallel inhomogeneities influence the course of Dco. In emphysema the time couse of Dco is best explained with a faster intrapulmonary mixing of He compared to CO. This behavior indicates that in emphysema low Dco values can be mainly attributed to large diffusional resistances (stratification) within the lungs.", "contents": "Dco at various breath-holding times: comparison in patients with chronic bronchial asthma and emphysema. Measurement of Dco is known to be dependent upon functional inhomogeneities. Because different types of inhomogeneities are operative in patients with bronchial asthma and patients with emphysema, different changes of Dco with increasing breath-holding time, tA, are to be expected. We studied the change of Dco with increasing breath-holding time in healthy subjects, patients with asthma bronchiale and patients with emphysema. In the patients the severity of airway obstruction was about the same. The following results were obtained: (a) in healthy subjects and in the asthmatics Dco decreased with tA, in a similar manner, approaching a value (ml . min-1 . Terror-1) of 34.7 and 31.6 at 10 sec, respectively, and (b) in patients with emphysema Dco increased with tA, yielding negative values at small tA: 1.5 sec-23.4; 10 sec: 11.7. From these results we suggest that in healthy subjects and in patients with bronchial asthma parallel inhomogeneities influence the course of Dco. In emphysema the time couse of Dco is best explained with a faster intrapulmonary mixing of He compared to CO. This behavior indicates that in emphysema low Dco values can be mainly attributed to large diffusional resistances (stratification) within the lungs."}
{"id": "PMID:472498", "title": "Effect of carboxyhemoglobin on the single breath diffusing capacity: derivation of an empirical correction factor.", "content": "The effect on measured DLco of different blood concentrations of COHb produced by breathing CO was studied in 19 healthy nonsmoking subjects. The DLco calculated without adjustment for blood COHb was found to be spuriously reduced by approximately 1% of the observed baseline value for each percent increase in blood COHb saturation. When measuring DLco of subjects who are likely to have elevated COHb blood levels, it is recommended that either blood COHb concentration or equilibrated alveolar PCO be determined in order to correct the DLco for the effect of COHb.", "contents": "Effect of carboxyhemoglobin on the single breath diffusing capacity: derivation of an empirical correction factor. The effect on measured DLco of different blood concentrations of COHb produced by breathing CO was studied in 19 healthy nonsmoking subjects. The DLco calculated without adjustment for blood COHb was found to be spuriously reduced by approximately 1% of the observed baseline value for each percent increase in blood COHb saturation. When measuring DLco of subjects who are likely to have elevated COHb blood levels, it is recommended that either blood COHb concentration or equilibrated alveolar PCO be determined in order to correct the DLco for the effect of COHb."}
{"id": "PMID:472499", "title": "Respiratory response to carbon dioxide after propranolol in normal subjects.", "content": "The ventilatory response to carbon dioxide, using the rebreathing technique, was investigated in 5 healthy nonsmoker volunteers, without obstructive bronchopathy. The administration of propranolol (20 mg) in a single oral dose did not produce significant modifications of the slopes of the response curves, but caused a significant increase of the intercept of the curves (p less than 0.05). Since no changes of the spirographic values were noted, the results obtained were attributed to a decrease of ventilation. It is concluded that propranolol, even at the dose of 20 mg, is able to induce a depression of the respiratory center, concomitant with significant reduction of heart rate and arterial blood pressure.", "contents": "Respiratory response to carbon dioxide after propranolol in normal subjects. The ventilatory response to carbon dioxide, using the rebreathing technique, was investigated in 5 healthy nonsmoker volunteers, without obstructive bronchopathy. The administration of propranolol (20 mg) in a single oral dose did not produce significant modifications of the slopes of the response curves, but caused a significant increase of the intercept of the curves (p less than 0.05). Since no changes of the spirographic values were noted, the results obtained were attributed to a decrease of ventilation. It is concluded that propranolol, even at the dose of 20 mg, is able to induce a depression of the respiratory center, concomitant with significant reduction of heart rate and arterial blood pressure."}
{"id": "PMID:472500", "title": "Effects of inhaled lidocaine on airway function in asthmatic subjects.", "content": "We measured the effect of inhaled lidocaine on pulmonary function in 8 asthmatic subjects. Plethysmographic specific airways conductance (SGaw) and the 1-sec forced expired volume (FEV1) were measured before and after the inhalation of 2cm3 of lidocaine (4%). Responses were also measured after patients were pretreated with either aerosolized isoproterenol, aerosolized atropine, or intramuscular atropine. In response to lidocaine alone, we observed a 23.4 +/- (SE) 4.8% fall in FEV1 and a 64.1 +/-(SE)3.8% fall in SGaw (p is less than 0.001). These effects were reversed with aerosolized atropine or isoproterenol. After pretreatment with aerosolized atropine or isoproterenol, the bronchoconstrictor effect of lidocaine were either prevented or markedly reduced. The protective effects of intramuscular atropine varied in different subjects, but in general, aerosolized bronchodilators afforded better protection against the bronchoconstrictor effect of lidocaine. Although lidocaine is theoretically capable of blocking neurogenic reflexes in the lung, our studies indicate that this topical anesthetic agent produces untoward reflex-mediated bronchoconstriction in patients with asthma and hyperirritable airways.", "contents": "Effects of inhaled lidocaine on airway function in asthmatic subjects. We measured the effect of inhaled lidocaine on pulmonary function in 8 asthmatic subjects. Plethysmographic specific airways conductance (SGaw) and the 1-sec forced expired volume (FEV1) were measured before and after the inhalation of 2cm3 of lidocaine (4%). Responses were also measured after patients were pretreated with either aerosolized isoproterenol, aerosolized atropine, or intramuscular atropine. In response to lidocaine alone, we observed a 23.4 +/- (SE) 4.8% fall in FEV1 and a 64.1 +/-(SE)3.8% fall in SGaw (p is less than 0.001). These effects were reversed with aerosolized atropine or isoproterenol. After pretreatment with aerosolized atropine or isoproterenol, the bronchoconstrictor effect of lidocaine were either prevented or markedly reduced. The protective effects of intramuscular atropine varied in different subjects, but in general, aerosolized bronchodilators afforded better protection against the bronchoconstrictor effect of lidocaine. Although lidocaine is theoretically capable of blocking neurogenic reflexes in the lung, our studies indicate that this topical anesthetic agent produces untoward reflex-mediated bronchoconstriction in patients with asthma and hyperirritable airways."}
{"id": "PMID:472501", "title": "[133 Xe radiospirometry: prediction of VC and FEV1 (author's transl)].", "content": "In patients with impaired lung function who are scheduled for lung surgery, a means of estimating the ventilatory consequences of parenchymal resection is needed. We have tested the ability of lung scanning with aerosolized and intravenous 133Xe to fulfil this purpose in 100 pneumonectomized or lobectomized patients. Postsurgery VC and FEV1 values were evaluated as follows: each lung was horizontally divided into two areas and lower and upper VC values were calculated as percentages of the total radioactivity count. Using the regional percentages and the absolute values of VC measured spirometrically, the VC expected postsurgery values were calculated according to the type of resection planned (e.g. if the left lung was to be removed and if it accounted for 50% of the radioactivity on the scan, the postsurgery VC would be halved). Postsurgery FEV1 values were estimated by using the VC value and the FEV1/VC ratio measured before surgery. A significant correlation (p less than 0.001) was found between the values estimated in such way and the actual FEV1 and VC values obtained shortly after surgery (between 6 and 15 days). From these results, we conclude that the xenon scan is a valuable technique for estimating the consequences of lung removal on ventilation.", "contents": "[133 Xe radiospirometry: prediction of VC and FEV1 (author's transl)]. In patients with impaired lung function who are scheduled for lung surgery, a means of estimating the ventilatory consequences of parenchymal resection is needed. We have tested the ability of lung scanning with aerosolized and intravenous 133Xe to fulfil this purpose in 100 pneumonectomized or lobectomized patients. Postsurgery VC and FEV1 values were evaluated as follows: each lung was horizontally divided into two areas and lower and upper VC values were calculated as percentages of the total radioactivity count. Using the regional percentages and the absolute values of VC measured spirometrically, the VC expected postsurgery values were calculated according to the type of resection planned (e.g. if the left lung was to be removed and if it accounted for 50% of the radioactivity on the scan, the postsurgery VC would be halved). Postsurgery FEV1 values were estimated by using the VC value and the FEV1/VC ratio measured before surgery. A significant correlation (p less than 0.001) was found between the values estimated in such way and the actual FEV1 and VC values obtained shortly after surgery (between 6 and 15 days). From these results, we conclude that the xenon scan is a valuable technique for estimating the consequences of lung removal on ventilation."}
{"id": "PMID:472502", "title": "Accumulation of 99mTc-tetracycline in lung infarction area. An experimental study.", "content": "In 10 dogs, lung infarction of the right middle and of the left inferior lobe was obtained experimentally. 1 h after operation, 2.0 mCi 99mtechnetium-tetracycline was given intravenously. 5 h after injection, a maximum of accumulation in the infarcted tissue was detected. Conventional perfusion scintigrams were done by injecting 0.2 mCi of 131iodine-albumin particles. Correspondingly, a perfusion reduction in the infarcted area was seen by this technique. This method described seems promising for clinical use for the detection of lung infarction. Clinical studies in this field are in progress.", "contents": "Accumulation of 99mTc-tetracycline in lung infarction area. An experimental study. In 10 dogs, lung infarction of the right middle and of the left inferior lobe was obtained experimentally. 1 h after operation, 2.0 mCi 99mtechnetium-tetracycline was given intravenously. 5 h after injection, a maximum of accumulation in the infarcted tissue was detected. Conventional perfusion scintigrams were done by injecting 0.2 mCi of 131iodine-albumin particles. Correspondingly, a perfusion reduction in the infarcted area was seen by this technique. This method described seems promising for clinical use for the detection of lung infarction. Clinical studies in this field are in progress."}
{"id": "PMID:472503", "title": "Bronchiectasis: a cause of pulmonary symptoms in heroin addicts.", "content": "Extensive and severe bronchiectasis was found in 7 heroin-addicted individuals with pulmonary symptoms whose chest roentgenograms were not suggestive of severe airway disease. Abnormalities consisted of varicose and cylindrical alterations. Pulmonary function tests revealed airflow obstruction, decreased lung volumes, and diffusion capacity impairment. Arterial blood gas analysis demonstrated mild hypoxemia in all patients and chronic hypocapnia in 4. Serial pulmonary function tests in 2 patients revealed only modest improvement in the degree of airflow obstruction. The occurence of bronchiectasis appeared to be related to episodes of heroin-induced pulmonary edema and infection.", "contents": "Bronchiectasis: a cause of pulmonary symptoms in heroin addicts. Extensive and severe bronchiectasis was found in 7 heroin-addicted individuals with pulmonary symptoms whose chest roentgenograms were not suggestive of severe airway disease. Abnormalities consisted of varicose and cylindrical alterations. Pulmonary function tests revealed airflow obstruction, decreased lung volumes, and diffusion capacity impairment. Arterial blood gas analysis demonstrated mild hypoxemia in all patients and chronic hypocapnia in 4. Serial pulmonary function tests in 2 patients revealed only modest improvement in the degree of airflow obstruction. The occurence of bronchiectasis appeared to be related to episodes of heroin-induced pulmonary edema and infection."}
{"id": "PMID:472504", "title": "Fibroleiomyomatous hamartoma of the lung.", "content": "Fibroleiomyomatous hamartoma of the lung is an extremely rare, nonneoplastic overgrowth of pulmonary muscle tissue which can be found in various sites throughout the lung. Several cases have been previously described. The case presently reviewed demonstrates the multinodular form of this entity. Fibroleiomyomatous hamartoma may also appear as a solitary coin lesion or as a diffuse interstitial pattern on chest radiographs.", "contents": "Fibroleiomyomatous hamartoma of the lung. Fibroleiomyomatous hamartoma of the lung is an extremely rare, nonneoplastic overgrowth of pulmonary muscle tissue which can be found in various sites throughout the lung. Several cases have been previously described. The case presently reviewed demonstrates the multinodular form of this entity. Fibroleiomyomatous hamartoma may also appear as a solitary coin lesion or as a diffuse interstitial pattern on chest radiographs."}
{"id": "PMID:472505", "title": "Radioisotopic labelling of endoalveolar surface-active phospholipidic fractions from rabbit pulmonary lavage.", "content": "A simple method of detecting endoalveolar tensioactive system fractions employing intravenously injected 131I-triolein has been devised. The tracer has been administered to 7 adult rabbit groups then sacrificed at different time intervals after the injection. Endoalveolar tensioactive system lipidic fractions collected by pulmonary lavage have been separated with thin-layer chromatography and their radioactivity evaluated by scanning plates. Endoalveolar tensioactive system fractions containing considerable amounts of C 18 fatty acids have been selectively labelled.", "contents": "Radioisotopic labelling of endoalveolar surface-active phospholipidic fractions from rabbit pulmonary lavage. A simple method of detecting endoalveolar tensioactive system fractions employing intravenously injected 131I-triolein has been devised. The tracer has been administered to 7 adult rabbit groups then sacrificed at different time intervals after the injection. Endoalveolar tensioactive system lipidic fractions collected by pulmonary lavage have been separated with thin-layer chromatography and their radioactivity evaluated by scanning plates. Endoalveolar tensioactive system fractions containing considerable amounts of C 18 fatty acids have been selectively labelled."}
{"id": "PMID:472506", "title": "Body plethysmographic measurements in children with an accompanying adult.", "content": "If a child refuses to enter the body plethysmograph, he may be accompanied by a relative or an assistant. Measurements are possible after the companion has stopped breathing and box pressure has stabilized. The reliability of this procedure is proved by a comparative examination of 25 children performed without and with a companion.", "contents": "Body plethysmographic measurements in children with an accompanying adult. If a child refuses to enter the body plethysmograph, he may be accompanied by a relative or an assistant. Measurements are possible after the companion has stopped breathing and box pressure has stabilized. The reliability of this procedure is proved by a comparative examination of 25 children performed without and with a companion."}
{"id": "PMID:472507", "title": "Small airways disease: a trial of an easy functional discrimination of preclinical emphysema.", "content": "In the genesis of small airways obstruction (SAO) we have to consider two alterations with different prognoses: the first, in which the obstruction depends on a reduction of the diameters of the small bronchi (i.e. with prevalent 'intrinsic mechanism)'), is reversible; the second, probably irreversible, is due to pulmonary emphysema in its pre-clinical stage. It is useful to distinguish between these two mechanisms by means of functional tests which are easily applicable in epidemiological screening. We have studied, by means of different lung mechanics tests, 5 normal, male, non-smoking subjects (N) and 10 male smokers considered to be affected by SAO. Several theoretical considerations, experimental data and prospective studies suggest that under various tests of easy execution for a screening program there are some which have, in some cases, a prognostic value and seem to be coupled to a qualitative meaning: this is the case for delta N2 which is significantly greater in subjects of the extrinsic group where the abnormality is considered irreversible. Some other tests are very sensitive to detect SAO subjects by they are lacking in specificity and in prognostic meaning (CV, VEmax, FDC). Finally, other tests [maximal mid-expiratory flow (MMEF) and residual volume (RV)] seem to have a prognostic value only because they indicate an advanced stage of the illness, not depending on the kind of the affection (SAO Ex or SAO In).", "contents": "Small airways disease: a trial of an easy functional discrimination of preclinical emphysema. In the genesis of small airways obstruction (SAO) we have to consider two alterations with different prognoses: the first, in which the obstruction depends on a reduction of the diameters of the small bronchi (i.e. with prevalent 'intrinsic mechanism)'), is reversible; the second, probably irreversible, is due to pulmonary emphysema in its pre-clinical stage. It is useful to distinguish between these two mechanisms by means of functional tests which are easily applicable in epidemiological screening. We have studied, by means of different lung mechanics tests, 5 normal, male, non-smoking subjects (N) and 10 male smokers considered to be affected by SAO. Several theoretical considerations, experimental data and prospective studies suggest that under various tests of easy execution for a screening program there are some which have, in some cases, a prognostic value and seem to be coupled to a qualitative meaning: this is the case for delta N2 which is significantly greater in subjects of the extrinsic group where the abnormality is considered irreversible. Some other tests are very sensitive to detect SAO subjects by they are lacking in specificity and in prognostic meaning (CV, VEmax, FDC). Finally, other tests [maximal mid-expiratory flow (MMEF) and residual volume (RV)] seem to have a prognostic value only because they indicate an advanced stage of the illness, not depending on the kind of the affection (SAO Ex or SAO In)."}
{"id": "PMID:472519", "title": "Chemoreceptor stimulation and hypoxic pulmonary vasoconstriction in conscious dogs.", "content": "Dogs with electromagnetic flow probes implanted on their left (QL) and main (QT) pulmonary arteries, catheters in their left atria and external jugular veins, and chronic tracheostomies were trained to accept Carlens dual-lumen endotracheal tubes into their tracheostomies, thus allowing separate ventilation of the two lungs. Swan-Ganz catheters were inserted through the jugular vein catheters. Pneumotachographs measured air flow to each lung. During bilateral ventilation with room air or O2, QL was about 36% of QT. When the left lung was ventilated with N2 while the right remained on O2, PAO2 was above 90 mmHg and QL fell to about 25% of QT. When the left lung was ventilated with N2 and the right with room air, PAO2 fell below 40 mm Hg and QL increased to control levels. This increase in perfusion of the hypoxic lung during systemic hypoxemia was not seen in dogs after surgical deafferentation of the systemic arterial chemoreceptors, indicating that stimulation of the arterial chemoreceptors may interfere with the hypoxic pulmonary vasoconstriction.", "contents": "Chemoreceptor stimulation and hypoxic pulmonary vasoconstriction in conscious dogs. Dogs with electromagnetic flow probes implanted on their left (QL) and main (QT) pulmonary arteries, catheters in their left atria and external jugular veins, and chronic tracheostomies were trained to accept Carlens dual-lumen endotracheal tubes into their tracheostomies, thus allowing separate ventilation of the two lungs. Swan-Ganz catheters were inserted through the jugular vein catheters. Pneumotachographs measured air flow to each lung. During bilateral ventilation with room air or O2, QL was about 36% of QT. When the left lung was ventilated with N2 while the right remained on O2, PAO2 was above 90 mmHg and QL fell to about 25% of QT. When the left lung was ventilated with N2 and the right with room air, PAO2 fell below 40 mm Hg and QL increased to control levels. This increase in perfusion of the hypoxic lung during systemic hypoxemia was not seen in dogs after surgical deafferentation of the systemic arterial chemoreceptors, indicating that stimulation of the arterial chemoreceptors may interfere with the hypoxic pulmonary vasoconstriction."}
{"id": "PMID:472520", "title": "A statistical description of the human tracheobronchial tree geometry.", "content": "Most physiological studies which made use of lung geometry have utilized average deterministic models of the tracheobronchial tree geometry, such as Weibel's Model A (1963). However, as shown by morphometric studies, it is well known that there are significant inter-subject and intra-subject variabilities in the structural components of the human lung. Hence, inherent inaccuracies exist when deterministic dimensions for lung geometry are used. In this paper, a statistical description of the lung geometry is presented. Using Weibel's Model A as the underlying average model, probability distributions for the lengths and the diameters of airways and for the number and volume of alveoli are proposed based on morphometric data. As a check for consistency, the probability distribution of the functional residual capacity is derived from those associated with airways and alveoli and it is compared with reported data. Results of this comparison are favorable, suggesting that the statistical description presented herein represents a self-consistent model for lung geometry which can be used for studies of problems related to pulmonary physiology.", "contents": "A statistical description of the human tracheobronchial tree geometry. Most physiological studies which made use of lung geometry have utilized average deterministic models of the tracheobronchial tree geometry, such as Weibel's Model A (1963). However, as shown by morphometric studies, it is well known that there are significant inter-subject and intra-subject variabilities in the structural components of the human lung. Hence, inherent inaccuracies exist when deterministic dimensions for lung geometry are used. In this paper, a statistical description of the lung geometry is presented. Using Weibel's Model A as the underlying average model, probability distributions for the lengths and the diameters of airways and for the number and volume of alveoli are proposed based on morphometric data. As a check for consistency, the probability distribution of the functional residual capacity is derived from those associated with airways and alveoli and it is compared with reported data. Results of this comparison are favorable, suggesting that the statistical description presented herein represents a self-consistent model for lung geometry which can be used for studies of problems related to pulmonary physiology."}
{"id": "PMID:472521", "title": "Trapped gas at maximum lung volume in intact isolated rat lungs.", "content": "Excised rat lungs were ventilated with air in a liquid filled plethysmograph that was enclosed in a large pressure chamber, C(B). The lungs were inflated then deflated by removing or adding saline to the plethysmograph while the trachea was attached to a cannula extending through the plethysmograph base. In this system, tracheal pressure, Pao, was equal to gas pressure inside C(B). The gas pressure was held constant at either ambient pressure (Pamb), Pamb + 350 Torr, or Pamb - 350 Torr. When excised lungs were ventilated slowly from their atelectatic state for 10 inflation-deflation cycles with Pao equal to any one of the three pressures, an equivalent amount of gas was trapped in the lungs. If after 10 cycles, however, lungs containing trapped gas were inflated and held at maximum lung volume, the trapped gas spaces could be made to expand in response to rarefaction or compression of the tracheal gas. The amount of expansion and contraction of the trapped gas spaces demonstrates that trapped gas is likely trapped between menisci of a foam that occlude the alveoli or small airways.", "contents": "Trapped gas at maximum lung volume in intact isolated rat lungs. Excised rat lungs were ventilated with air in a liquid filled plethysmograph that was enclosed in a large pressure chamber, C(B). The lungs were inflated then deflated by removing or adding saline to the plethysmograph while the trachea was attached to a cannula extending through the plethysmograph base. In this system, tracheal pressure, Pao, was equal to gas pressure inside C(B). The gas pressure was held constant at either ambient pressure (Pamb), Pamb + 350 Torr, or Pamb - 350 Torr. When excised lungs were ventilated slowly from their atelectatic state for 10 inflation-deflation cycles with Pao equal to any one of the three pressures, an equivalent amount of gas was trapped in the lungs. If after 10 cycles, however, lungs containing trapped gas were inflated and held at maximum lung volume, the trapped gas spaces could be made to expand in response to rarefaction or compression of the tracheal gas. The amount of expansion and contraction of the trapped gas spaces demonstrates that trapped gas is likely trapped between menisci of a foam that occlude the alveoli or small airways."}
{"id": "PMID:472522", "title": "Experimentally induced Cheyne-Stokes breathing.", "content": "We have studied the propensity for periodic breathing to occur in cats anaesthetized with pentobarbitone breathing either spontaneously or with the aid of a 'servo-respirator' governed continuously by the efferent phrenic nerve activity. Sustained periodic breathing could be induced increasing 'controller gain', either by increasing the gain of the respirator, or by lung deflation, which reflexly increased controller responses to both hypoxia and hypercapnia. Periodic breathing was potentiated both by hypoxia and by diminishing the central (CO2, H+)-drive by focal cooling at the ventral surface of the medulla, two procedures which increase the relative influence of hypoxic drive. Less hypoxia was needed to produce periodic breathing at high rather than low controller gains. Reducing controller gain to zero by constant artificial respiration always abolished periodic breathing. Periodic breathing was also eradicated when the relative importance of CO2 drive was enhanced by breathing the cats with CO2-enriched gas mixtures or with 100% O2. The results are consistent with theoretical predictions for the occurrence of oscillations in the mechanisms for the chemical control of breathing and indicate that increasing controller gas can produce periodic breathing. The results further emphasize the importance of the (CO2, H+)-drive in preserving ventilatory stability.", "contents": "Experimentally induced Cheyne-Stokes breathing. We have studied the propensity for periodic breathing to occur in cats anaesthetized with pentobarbitone breathing either spontaneously or with the aid of a 'servo-respirator' governed continuously by the efferent phrenic nerve activity. Sustained periodic breathing could be induced increasing 'controller gain', either by increasing the gain of the respirator, or by lung deflation, which reflexly increased controller responses to both hypoxia and hypercapnia. Periodic breathing was potentiated both by hypoxia and by diminishing the central (CO2, H+)-drive by focal cooling at the ventral surface of the medulla, two procedures which increase the relative influence of hypoxic drive. Less hypoxia was needed to produce periodic breathing at high rather than low controller gains. Reducing controller gain to zero by constant artificial respiration always abolished periodic breathing. Periodic breathing was also eradicated when the relative importance of CO2 drive was enhanced by breathing the cats with CO2-enriched gas mixtures or with 100% O2. The results are consistent with theoretical predictions for the occurrence of oscillations in the mechanisms for the chemical control of breathing and indicate that increasing controller gas can produce periodic breathing. The results further emphasize the importance of the (CO2, H+)-drive in preserving ventilatory stability."}
{"id": "PMID:472523", "title": "O2-chemoreflex drive of ventilation in awake birds at rest.", "content": "Three varieties of birds (a non-flyer, the chicken; a diver, the duck; a flyer, the pigeon) were studied in resting conditions at neutral ambient temperature, while awake, either intact or sham operated, or after bilateral chronic carotid body denervation. Tidal volume (VT), ventilatory period (T) and minute volume (V = VT/T) measured plethysmographically, were recorded breath-by-breath in steady state at two levels of oxygenation, and in the course of transient pure O2 inhalation (60-sec O2-test). O2 partial pressures in the inspired and expired gases (PIO2 and PETO2), and in the arterial blood (PaO2) were also measured. In intact and sham operated birds, the abrupt switch from a normoxic gas mixture (FIO2 = 0.21) to pure O2 resulted shortly in rapid increases of PIO2, PETO2 and paO2, and in a fall of V which was completed within 20--30 sec and accounted for about 30% of the control minute volume. In the animals previously made hypoxic (FIO2 = 0.12) and hyperventilating, the O2-test provoked a 50--60% fall of V. In chickens, a decreased VT and an increased T contributed to the transient ventilatory changes; in ducks, mainly VT changed, and in the pigeon only T changed. In the birds with denervated carotid bodies, there were no ventilatory responses to hypoxia and to the O2-test. A few of these birds developed a tachypneic response to hypoxia with no apparent change in the effective pulmonary ventilation, which was partly overcome during the O2-test. It is concluded that in the three varieties of birds, the O2-chemoreflex drive from the carotid bodies controlled about 30% of the resting minute volume near sea level, and 50--60% in hypoxic conditions duplicating an altitude exposure to 4000 m.", "contents": "O2-chemoreflex drive of ventilation in awake birds at rest. Three varieties of birds (a non-flyer, the chicken; a diver, the duck; a flyer, the pigeon) were studied in resting conditions at neutral ambient temperature, while awake, either intact or sham operated, or after bilateral chronic carotid body denervation. Tidal volume (VT), ventilatory period (T) and minute volume (V = VT/T) measured plethysmographically, were recorded breath-by-breath in steady state at two levels of oxygenation, and in the course of transient pure O2 inhalation (60-sec O2-test). O2 partial pressures in the inspired and expired gases (PIO2 and PETO2), and in the arterial blood (PaO2) were also measured. In intact and sham operated birds, the abrupt switch from a normoxic gas mixture (FIO2 = 0.21) to pure O2 resulted shortly in rapid increases of PIO2, PETO2 and paO2, and in a fall of V which was completed within 20--30 sec and accounted for about 30% of the control minute volume. In the animals previously made hypoxic (FIO2 = 0.12) and hyperventilating, the O2-test provoked a 50--60% fall of V. In chickens, a decreased VT and an increased T contributed to the transient ventilatory changes; in ducks, mainly VT changed, and in the pigeon only T changed. In the birds with denervated carotid bodies, there were no ventilatory responses to hypoxia and to the O2-test. A few of these birds developed a tachypneic response to hypoxia with no apparent change in the effective pulmonary ventilation, which was partly overcome during the O2-test. It is concluded that in the three varieties of birds, the O2-chemoreflex drive from the carotid bodies controlled about 30% of the resting minute volume near sea level, and 50--60% in hypoxic conditions duplicating an altitude exposure to 4000 m."}
{"id": "PMID:472594", "title": "Particulate emboli retained by the Intersept (R) transfusion filter; a sem study.", "content": "The Intersept (R) transfusion filter is the transfusion filter developed by Johnson and Johnson. Filtration is performed by both adsorption and mechanical retention. Not only microaggregates, but damaged and injured cells are likely to be trapped and/or adsorbed. Such a filter is not injurious to the blood components, but thrombotic material accumulation can occur with fresh blood.", "contents": "Particulate emboli retained by the Intersept (R) transfusion filter; a sem study. The Intersept (R) transfusion filter is the transfusion filter developed by Johnson and Johnson. Filtration is performed by both adsorption and mechanical retention. Not only microaggregates, but damaged and injured cells are likely to be trapped and/or adsorbed. Such a filter is not injurious to the blood components, but thrombotic material accumulation can occur with fresh blood."}
{"id": "PMID:472595", "title": "[Fluorocarbons as blood substitutes. Toxicity in the rat].", "content": "Liquid fluorocarbons, having high solubility for gases (O2, CO2...) have been used as artificial blood substitutes in animals with variable results. The great diversity of these products and the lack of reproductiveness in their composition have not permitted, up to now, a standardization of their utilisation norms. Our work was to study the toxicity, in the rat, of a new kind of fluorocarbon emulsion (E-66, Ugine-Kuhlmann, France) used as an artificial blood substitute during exchange-perfusion. The short survival of the rats is in opposition to the good in vitro results obtained in other experiments (high solubilities of oxygen and dioxide carbon). The toxicity of this fluorinated emulsion is demonstrated by histologic lesions in lungs, liver and kidneys and the great amount of fluor stored in these organs. The mechanism of this toxicity is still to be demonstrated. Hepatic lesions (hyperhemia with dilatation of central veins and sinusoids) and pulmonary lesions (vascular congestion, alveolar oedema) prove a circulatory disturbance leading to right-sided cardiac failure. However, cellular degenerescence lesions, observed in hepatocytes and renal tubular cells, do not permit to exclude formally a cellular toxicity of the E-66 emulsion.", "contents": "[Fluorocarbons as blood substitutes. Toxicity in the rat]. Liquid fluorocarbons, having high solubility for gases (O2, CO2...) have been used as artificial blood substitutes in animals with variable results. The great diversity of these products and the lack of reproductiveness in their composition have not permitted, up to now, a standardization of their utilisation norms. Our work was to study the toxicity, in the rat, of a new kind of fluorocarbon emulsion (E-66, Ugine-Kuhlmann, France) used as an artificial blood substitute during exchange-perfusion. The short survival of the rats is in opposition to the good in vitro results obtained in other experiments (high solubilities of oxygen and dioxide carbon). The toxicity of this fluorinated emulsion is demonstrated by histologic lesions in lungs, liver and kidneys and the great amount of fluor stored in these organs. The mechanism of this toxicity is still to be demonstrated. Hepatic lesions (hyperhemia with dilatation of central veins and sinusoids) and pulmonary lesions (vascular congestion, alveolar oedema) prove a circulatory disturbance leading to right-sided cardiac failure. However, cellular degenerescence lesions, observed in hepatocytes and renal tubular cells, do not permit to exclude formally a cellular toxicity of the E-66 emulsion."}
{"id": "PMID:472596", "title": "[Biological changes observed after infusion of modified gelatin in women who had cesarean sections].", "content": "We suggest to study some biologic parameters after perfusion by 1,000 ml modified gelatin to young women who have a caesarian operation. The level of gelatin is determined by previously proposed method. Gelatin is also revealed at electrophoresis. Concurrently, protein contents in sera and hematocrit are estimated. These investigations are also made at the same time for a reference population who has a ceasarian operation without perfusion of gelatin. The highest level of gelatin is reached at t(0) + 90 mn. Gelatin is not present in sera at t(0) + 6 hr. At electrophoresis the beta-globulins raise concurrently with the level of gelatin. The alpha-globulins decrease but that variation is small and later. Protein level decreases also in conjunction with hematocrit. Hematocrit value is about 30 p. cent. It seems to the authors that this value allows a better oxygen transmission to tissues. For reference population these biologic parameters are not modified.", "contents": "[Biological changes observed after infusion of modified gelatin in women who had cesarean sections]. We suggest to study some biologic parameters after perfusion by 1,000 ml modified gelatin to young women who have a caesarian operation. The level of gelatin is determined by previously proposed method. Gelatin is also revealed at electrophoresis. Concurrently, protein contents in sera and hematocrit are estimated. These investigations are also made at the same time for a reference population who has a ceasarian operation without perfusion of gelatin. The highest level of gelatin is reached at t(0) + 90 mn. Gelatin is not present in sera at t(0) + 6 hr. At electrophoresis the beta-globulins raise concurrently with the level of gelatin. The alpha-globulins decrease but that variation is small and later. Protein level decreases also in conjunction with hematocrit. Hematocrit value is about 30 p. cent. It seems to the authors that this value allows a better oxygen transmission to tissues. For reference population these biologic parameters are not modified."}
{"id": "PMID:472597", "title": "[Detection of lipid abnormalities in blood donors].", "content": "The high incidence of cardio- or cerebro-vascular diseases is positively correlated with hyperlipoproteinemia. A large-scale screening of blood donor's populations could be used for the prevention of the atherogenic disease. Therefore lipoproteins electrophoresis on cellogel was compared with serum levels of triglycerides, cholesterol and lipids in 1184 blood donors (792 men, 392 women). The electrophoretic pattern was found abnormal in 32 cases (25 men, 7 women). It was a type IIb hyperlipoproteinemia, according to the classification of the World Health Organization. In these 32 subjects, serum triglycerides, cholesterol and lipids concentrations were significantly higher (p less than 0,001) than in 41 other donors with a normal electrophoretic pattern. A good positive correlation was found between high blood pressure or obesity or blood group O and abnormal electrophoretic pattern. Lipoproteins electrophoresis on cellogel appears to be a suitable test (easy, fast and economical) in large-scale screening for dyslipidemia in subjects over 40, or at least in cases of mild hypertension or obesity.", "contents": "[Detection of lipid abnormalities in blood donors]. The high incidence of cardio- or cerebro-vascular diseases is positively correlated with hyperlipoproteinemia. A large-scale screening of blood donor's populations could be used for the prevention of the atherogenic disease. Therefore lipoproteins electrophoresis on cellogel was compared with serum levels of triglycerides, cholesterol and lipids in 1184 blood donors (792 men, 392 women). The electrophoretic pattern was found abnormal in 32 cases (25 men, 7 women). It was a type IIb hyperlipoproteinemia, according to the classification of the World Health Organization. In these 32 subjects, serum triglycerides, cholesterol and lipids concentrations were significantly higher (p less than 0,001) than in 41 other donors with a normal electrophoretic pattern. A good positive correlation was found between high blood pressure or obesity or blood group O and abnormal electrophoretic pattern. Lipoproteins electrophoresis on cellogel appears to be a suitable test (easy, fast and economical) in large-scale screening for dyslipidemia in subjects over 40, or at least in cases of mild hypertension or obesity."}
{"id": "PMID:472614", "title": "[Physiopathology of ischemic transitory attacks in the vertebro-basilar region. II. Physiopathological and clinical aspects].", "content": "Hemodynamic and metabolic factors of regional cerebral blood flow regulation are analyzed, and pathophysiology and clinical aspects of transient ischemic attacks of vertebro-basilar system are discussed.", "contents": "[Physiopathology of ischemic transitory attacks in the vertebro-basilar region. II. Physiopathological and clinical aspects]. Hemodynamic and metabolic factors of regional cerebral blood flow regulation are analyzed, and pathophysiology and clinical aspects of transient ischemic attacks of vertebro-basilar system are discussed."}
{"id": "PMID:472613", "title": "[Physiology of transitory ischemic attacks in the vertebro-basilar region. I. Functional anatomy of the vertebro-basilar system].", "content": "Regions of supply by the posterior segment of the circle of Willis are discussed in terms of anatomo-functional organization.", "contents": "[Physiology of transitory ischemic attacks in the vertebro-basilar region. I. Functional anatomy of the vertebro-basilar system]. Regions of supply by the posterior segment of the circle of Willis are discussed in terms of anatomo-functional organization."}
{"id": "PMID:472616", "title": "[Hematic radio-immunologic monitoring of clonazepam: clinical-pharmacological evaluations in a group of epileptic patients].", "content": "In a group of 22 epileptic patients under treatment with clonazepam (CNP) it has been evaluated the serum levels of CNP in a seried way for a period of several months, by a radio-immunological technique. Except for one patient, all the others were under politherapy. On the basis of the data obtained from this study we tried to evaluate the pharmachological action of CNP. Even if we found a good clinical efficacy of CNP with therapeutic ranges (from 19,57 +/- 10,98 ng/ml up to 60,16 +/- 31,18 ng/ml) we didn't find any correlation between the oral dose and serum level, taking into account also other parameters such as sex, pound and age. The Authors discuss the possible factors which may be thought involved in this lacking correlation.", "contents": "[Hematic radio-immunologic monitoring of clonazepam: clinical-pharmacological evaluations in a group of epileptic patients]. In a group of 22 epileptic patients under treatment with clonazepam (CNP) it has been evaluated the serum levels of CNP in a seried way for a period of several months, by a radio-immunological technique. Except for one patient, all the others were under politherapy. On the basis of the data obtained from this study we tried to evaluate the pharmachological action of CNP. Even if we found a good clinical efficacy of CNP with therapeutic ranges (from 19,57 +/- 10,98 ng/ml up to 60,16 +/- 31,18 ng/ml) we didn't find any correlation between the oral dose and serum level, taking into account also other parameters such as sex, pound and age. The Authors discuss the possible factors which may be thought involved in this lacking correlation."}
{"id": "PMID:472615", "title": "[Gerstmann's syndrome: report of a case].", "content": "In recent years, several authors have questioned the reliaty of Gerstmann syndrome and have claimed that \"aphasia might be regarded as the true Grundst\u00f6rung of the Gerstmann symptoms\". This paper describes a case of Gerstmann syndrome ictally risen with aphasia in a patient suffering from a tumour of the left PTO region. After the remission of aphasia, the syndromic association typical of the Gerstmann syndrome lasted up the exitus.", "contents": "[Gerstmann's syndrome: report of a case]. In recent years, several authors have questioned the reliaty of Gerstmann syndrome and have claimed that \"aphasia might be regarded as the true Grundst\u00f6rung of the Gerstmann symptoms\". This paper describes a case of Gerstmann syndrome ictally risen with aphasia in a patient suffering from a tumour of the left PTO region. After the remission of aphasia, the syndromic association typical of the Gerstmann syndrome lasted up the exitus."}
{"id": "PMID:472635", "title": "Application of mathematical methods to the study of familial aggregation in endemic nephropathy. I. The hypothesis of infectious etiology.", "content": "Among many etiological factors, infection and the genetic factor are the main two causes which could explain the familial aggregation of endemic nephropathy (EN). The data collected over all the EN cases during 1957--1976 in the endemic village Bistri\u0163a have been analysed for time-space clustering and a particular type of spatial clustering (within household) by the Knox's space-time interaction test and Walter's pair statistic test. The lack of clustering could be interpreted as suggesting the lack of infection (human transmission pattern) in the occurrence of endemic nephropathy.", "contents": "Application of mathematical methods to the study of familial aggregation in endemic nephropathy. I. The hypothesis of infectious etiology. Among many etiological factors, infection and the genetic factor are the main two causes which could explain the familial aggregation of endemic nephropathy (EN). The data collected over all the EN cases during 1957--1976 in the endemic village Bistri\u0163a have been analysed for time-space clustering and a particular type of spatial clustering (within household) by the Knox's space-time interaction test and Walter's pair statistic test. The lack of clustering could be interpreted as suggesting the lack of infection (human transmission pattern) in the occurrence of endemic nephropathy."}
{"id": "PMID:472636", "title": "Study on the onset of atherosclerotic lesions in human coronary arteries.", "content": "The coronary arteries of 300 fetuses, neonates, infants and children were examined by light microscope in an effort to reconstruct the natural history of the first atherosclerotic lesions. The critical period for the passage from processes of growth and remodelling to atherosclerotic involvement appeared in this series at the age of 5 years. The incidence of the earliest lesions was distributed as follows: fibrous plaques in 2% of the subjects 6--10 years old and 4% of the subjects 11--15 years old; gelatinous plaques and fatty streaks in 6% of the subjects 11--15 years old. Indirect evidence was obtained that certain branch pads or cushions acted as precursors of the first fibrous plaques. Their conversion into atherosclerotic lesions was induced by insudation, histolysis (elastolysis, collagenolysis, ground substance depletion and degenerative cell changes), followed by nodular proliferation of smooth muscle cells and abundant neoformation of ground substance and collagen fibers. The onset of the first gelatinous plaques was also preceded by insudation and histolysis followed by swelling necrosis. The appearance of the first fatty streaks seemed to be not preceded by particular structural changes. The first fibrous plaques developed only in bifurcation and emergence areas, whereas the first gelatinous plaques and fatty streaks involved the proximal segments of the main coronary vessels.", "contents": "Study on the onset of atherosclerotic lesions in human coronary arteries. The coronary arteries of 300 fetuses, neonates, infants and children were examined by light microscope in an effort to reconstruct the natural history of the first atherosclerotic lesions. The critical period for the passage from processes of growth and remodelling to atherosclerotic involvement appeared in this series at the age of 5 years. The incidence of the earliest lesions was distributed as follows: fibrous plaques in 2% of the subjects 6--10 years old and 4% of the subjects 11--15 years old; gelatinous plaques and fatty streaks in 6% of the subjects 11--15 years old. Indirect evidence was obtained that certain branch pads or cushions acted as precursors of the first fibrous plaques. Their conversion into atherosclerotic lesions was induced by insudation, histolysis (elastolysis, collagenolysis, ground substance depletion and degenerative cell changes), followed by nodular proliferation of smooth muscle cells and abundant neoformation of ground substance and collagen fibers. The onset of the first gelatinous plaques was also preceded by insudation and histolysis followed by swelling necrosis. The appearance of the first fatty streaks seemed to be not preceded by particular structural changes. The first fibrous plaques developed only in bifurcation and emergence areas, whereas the first gelatinous plaques and fatty streaks involved the proximal segments of the main coronary vessels."}
{"id": "PMID:472639", "title": "Comparative clinical study of the effects of Na isobutyrate and clofibrate on the clotting factors in patients with atherosclerosis.", "content": "The effects of Na isobutyrate and clofibrate on human hemostasis were studied in 24 patients with atherosclerosis. Significant changes were recorded only with respect to platelet adhesiveness and blood fibrinogen concentrations. Thus, the index of platelet adhesiveness decreased significantly in 17 out of 19 patients with high initial values, after six weeks of Na isobutyrate therapy (1 g/day), and in 9 out of 17 patients, after clofibrate administration (1.5 g/day) in the same conditions. Blood fibrinogen concentrations were significantly lowered by clofibrate therapy and only slightly influenced by Na isobutyrate.", "contents": "Comparative clinical study of the effects of Na isobutyrate and clofibrate on the clotting factors in patients with atherosclerosis. The effects of Na isobutyrate and clofibrate on human hemostasis were studied in 24 patients with atherosclerosis. Significant changes were recorded only with respect to platelet adhesiveness and blood fibrinogen concentrations. Thus, the index of platelet adhesiveness decreased significantly in 17 out of 19 patients with high initial values, after six weeks of Na isobutyrate therapy (1 g/day), and in 9 out of 17 patients, after clofibrate administration (1.5 g/day) in the same conditions. Blood fibrinogen concentrations were significantly lowered by clofibrate therapy and only slightly influenced by Na isobutyrate."}
{"id": "PMID:472640", "title": "Morphogenesis and pathogenesis of chronic lung diseases. XVI. The influence of drugs on the stereotyped development and cellular kinetics of experimental lung granulomas.", "content": "The stereotyped development and the cellular kinetics of lung granulomas experimentally-induced in rabbits by an intravenous injection of the complete Freund adjuvant with addition of human gammaglobulin, were studied under the influence of hydrocortisone acetate, rifampicin, heparin and phenobarbital. As in a previous study using actinomycin and puromycin, the analysed drugs delayed up to inhibition (hydrocortisone) the development of lung granulomas which remained small, unfused, without epithelioidosis; the primary and secondary immune responses were more or less influenced up to their suppression. The delivery of macrophages was reduced by hydrocortisone, but enhanced by heparin and phenobarbital. The latter induced a persistent intragranulomatous granulocytosis, expressing the repair of decreased activities of dehydrogenases and especially of NADP-NADPH-oxidases. The action of these drugs was phasic, their intervention being active only during the cellular phase of granulomas; fibrillogenesis was influenced only indirectly. Extrapolation to humans of these results suggests the use of these drugs during the cellular phase of chronic pneumonitic lesions and suppression of the immune component of the inflammatory processes.", "contents": "Morphogenesis and pathogenesis of chronic lung diseases. XVI. The influence of drugs on the stereotyped development and cellular kinetics of experimental lung granulomas. The stereotyped development and the cellular kinetics of lung granulomas experimentally-induced in rabbits by an intravenous injection of the complete Freund adjuvant with addition of human gammaglobulin, were studied under the influence of hydrocortisone acetate, rifampicin, heparin and phenobarbital. As in a previous study using actinomycin and puromycin, the analysed drugs delayed up to inhibition (hydrocortisone) the development of lung granulomas which remained small, unfused, without epithelioidosis; the primary and secondary immune responses were more or less influenced up to their suppression. The delivery of macrophages was reduced by hydrocortisone, but enhanced by heparin and phenobarbital. The latter induced a persistent intragranulomatous granulocytosis, expressing the repair of decreased activities of dehydrogenases and especially of NADP-NADPH-oxidases. The action of these drugs was phasic, their intervention being active only during the cellular phase of granulomas; fibrillogenesis was influenced only indirectly. Extrapolation to humans of these results suggests the use of these drugs during the cellular phase of chronic pneumonitic lesions and suppression of the immune component of the inflammatory processes."}
{"id": "PMID:472641", "title": "Subphrenic abscess simulating metastatic carcinoma.", "content": "Subphrenic abscess is reported in two patients, one previously operated on for pancreatic carcinoma and the other for clear cell carcinoma of the left kidney. The subphrenic abscess presented with cachexia and low grade fever six months and one year after surgery. Metastatic carcinoma was erroneously diagnosed in both patients. Despite massive antibiotic treatment, both patients succumbed to sepsis. Because of the inherent diagnostic challenge, delineation of a subphrenic abscess in cancer patients without clear-cut evidence of a metastatic spread is crucial.", "contents": "Subphrenic abscess simulating metastatic carcinoma. Subphrenic abscess is reported in two patients, one previously operated on for pancreatic carcinoma and the other for clear cell carcinoma of the left kidney. The subphrenic abscess presented with cachexia and low grade fever six months and one year after surgery. Metastatic carcinoma was erroneously diagnosed in both patients. Despite massive antibiotic treatment, both patients succumbed to sepsis. Because of the inherent diagnostic challenge, delineation of a subphrenic abscess in cancer patients without clear-cut evidence of a metastatic spread is crucial."}
{"id": "PMID:472646", "title": "[Disfiguring ulcer forms of the carpal tunnel syndrome].", "content": "On the basis of 3 personal cases and 13 cases in the literature, the authors carried out a study of these rare forms of the carpal tunnel syndrome. The syndrome itself generally has a unique character marked on the etiologic level by a frequent occurrence of unilateral and post-traumatic forms, and on the clinical level by the existence of severe sensitivo-motor, vasomotor and trophic problems; it rarely resembles Raynaud's syndrome. The cutaneous lesion most often appears as a torpid ulceration located on the forefinger and/or the second finger and involving the palmar surface or the sub-ungual region of the 3rd phalanx. Cutting of the ligament of the middle finger, whether or not accompanied by an additional surgical act, is almost always followed by a regression of the classical signs and the curing of the ulcerations.", "contents": "[Disfiguring ulcer forms of the carpal tunnel syndrome]. On the basis of 3 personal cases and 13 cases in the literature, the authors carried out a study of these rare forms of the carpal tunnel syndrome. The syndrome itself generally has a unique character marked on the etiologic level by a frequent occurrence of unilateral and post-traumatic forms, and on the clinical level by the existence of severe sensitivo-motor, vasomotor and trophic problems; it rarely resembles Raynaud's syndrome. The cutaneous lesion most often appears as a torpid ulceration located on the forefinger and/or the second finger and involving the palmar surface or the sub-ungual region of the 3rd phalanx. Cutting of the ligament of the middle finger, whether or not accompanied by an additional surgical act, is almost always followed by a regression of the classical signs and the curing of the ulcerations."}
{"id": "PMID:472642", "title": "Serologic diagnosis in the differentiation of autoimmune hemolytic anemias.", "content": "A complex serologic study was carried out in 20 patients with autoimmune hemolytic anemias (AIHA). The methods used were: the direct Coombs' test with specific sera anti IgG, IgM, IgA and complement (C), the direct and indirect test with papain treated erythrocytes (at 37 degrees C), determination of cold agglutinin titer and of warm and cold hemolysins. By these investigations using indigenous sera, the 20 cases of autoimmune hemolytic anemia could be classified: a) according to the thermal behaviour of autoantibodies, into warm antibody AIHA -- 15 cases, cold antibody AIHA -- 2 cases and both warm and cold antibody AIHA -- 3 cases; and b) according to the sensitizing globulin, into: IgG type -- 10 cases, IgG + C type -- 7 cases, IgG + IgM + C type -- 1 case, type C + cold agglutinins -- 2 cases.", "contents": "Serologic diagnosis in the differentiation of autoimmune hemolytic anemias. A complex serologic study was carried out in 20 patients with autoimmune hemolytic anemias (AIHA). The methods used were: the direct Coombs' test with specific sera anti IgG, IgM, IgA and complement (C), the direct and indirect test with papain treated erythrocytes (at 37 degrees C), determination of cold agglutinin titer and of warm and cold hemolysins. By these investigations using indigenous sera, the 20 cases of autoimmune hemolytic anemia could be classified: a) according to the thermal behaviour of autoantibodies, into warm antibody AIHA -- 15 cases, cold antibody AIHA -- 2 cases and both warm and cold antibody AIHA -- 3 cases; and b) according to the sensitizing globulin, into: IgG type -- 10 cases, IgG + C type -- 7 cases, IgG + IgM + C type -- 1 case, type C + cold agglutinins -- 2 cases."}
{"id": "PMID:472643", "title": "Value of the test of spontaneous reduction of nitroblue tetrazolium in diabetes decompensation.", "content": "Nitroblue tetrazolium (NBT) test was performed in forty-four diabetic patients of both sexes ranging in age from 15 to 64 years. Some of the patients were tested both before and after diabetes compensation. In cases of decompensated diabetes caused by dietary errors or by inadequate dosage of the antidiabetic drugs, NBT results were within normal range. Significantly higher values were observed in decompensation of diabetes caused by bacterial infection. This observation may be used to detect the real cause of diabetes decompensation.", "contents": "Value of the test of spontaneous reduction of nitroblue tetrazolium in diabetes decompensation. Nitroblue tetrazolium (NBT) test was performed in forty-four diabetic patients of both sexes ranging in age from 15 to 64 years. Some of the patients were tested both before and after diabetes compensation. In cases of decompensated diabetes caused by dietary errors or by inadequate dosage of the antidiabetic drugs, NBT results were within normal range. Significantly higher values were observed in decompensation of diabetes caused by bacterial infection. This observation may be used to detect the real cause of diabetes decompensation."}
{"id": "PMID:472653", "title": "Erythropoiesis in sickle cell anaemia during acute infection and crisis.", "content": "Erythropoiesis was evaluated in 37 patients with sickle cell anaemia, 26 of them children under 12 years of age. Mean haemoglobin, haematocrit, reticulocyte, and erythropoietin levels were similar for 11 who were asymptomatic, 11 with infections, and 12 in vaso-occlusive crisis. Mean haemoglobin, haematocrit, and reticulocyte values were significantly lower and the mean erythropoietin level significantly higher for three patients in aplastic crisis. Reticulocyte counts reflected erythropoietic activity during the asymptomatic state but were variable during infection and crisis. No erythropoietic inhibitory activity was found in any of the four clinical states. It has been suggested that erythropoietin production decreases during infection. Patients in this study responded appropriately to stress, showing no decrease in erythropoietic activity during acute infection or crisis.", "contents": "Erythropoiesis in sickle cell anaemia during acute infection and crisis. Erythropoiesis was evaluated in 37 patients with sickle cell anaemia, 26 of them children under 12 years of age. Mean haemoglobin, haematocrit, reticulocyte, and erythropoietin levels were similar for 11 who were asymptomatic, 11 with infections, and 12 in vaso-occlusive crisis. Mean haemoglobin, haematocrit, and reticulocyte values were significantly lower and the mean erythropoietin level significantly higher for three patients in aplastic crisis. Reticulocyte counts reflected erythropoietic activity during the asymptomatic state but were variable during infection and crisis. No erythropoietic inhibitory activity was found in any of the four clinical states. It has been suggested that erythropoietin production decreases during infection. Patients in this study responded appropriately to stress, showing no decrease in erythropoietic activity during acute infection or crisis."}
{"id": "PMID:472654", "title": "The effect of sonicated serum on growth of normal and leukaemic human cells in agar cultures.", "content": "The existence of circulating factors in blood of possible importance for granulopoiesis was investigated by examining the effect of sonicated serum on agar cultures of human haematopoietic cells. Ultrasound treatment of serum can activate enzymes normally bound to carrier proteins. In normal bone marrow cultures, growth was inhibited by sonicated serum when the cells were cultured in a single layer without exogenous colony-stimulating factor (CSF) included in the culture, while an enhancing effect with a 2--5 fold increase in the number of colonies was seen in feeder layer-stimulated cultures. Morphologically, in contrast to the normal change to eosinophils and monocytes-macrophages during the culture period, the cultures with sonicated serum added showed continuous growth of neutrophils and no increase of other cell types. Experiments using tritiated thymidine indicated that the enhancing effect of sonicated serum involved marrow cells which were more prone to thymidine S-phase kill. The effect of sonicated serum was further evaluated in cultures from patients with leukaemia. In AML in relapse, feeder-layer stimulated cultures of bone marrow cells were inhibited (11/14 cases) by sonicated serum, while reversion to the normal enhancing pattern was seen for patients in remission.", "contents": "The effect of sonicated serum on growth of normal and leukaemic human cells in agar cultures. The existence of circulating factors in blood of possible importance for granulopoiesis was investigated by examining the effect of sonicated serum on agar cultures of human haematopoietic cells. Ultrasound treatment of serum can activate enzymes normally bound to carrier proteins. In normal bone marrow cultures, growth was inhibited by sonicated serum when the cells were cultured in a single layer without exogenous colony-stimulating factor (CSF) included in the culture, while an enhancing effect with a 2--5 fold increase in the number of colonies was seen in feeder layer-stimulated cultures. Morphologically, in contrast to the normal change to eosinophils and monocytes-macrophages during the culture period, the cultures with sonicated serum added showed continuous growth of neutrophils and no increase of other cell types. Experiments using tritiated thymidine indicated that the enhancing effect of sonicated serum involved marrow cells which were more prone to thymidine S-phase kill. The effect of sonicated serum was further evaluated in cultures from patients with leukaemia. In AML in relapse, feeder-layer stimulated cultures of bone marrow cells were inhibited (11/14 cases) by sonicated serum, while reversion to the normal enhancing pattern was seen for patients in remission."}
{"id": "PMID:472655", "title": "Blood colony-forming cells (CFU-C) and leucocyte colony-stimulating activity (CSA) in patients with neutrophilic leucocytosis.", "content": "The incidence of circulating colony-forming cells (CFU-C) and the ability of peripheral leucocytes to stimulate the colony formation (CSA) have been studied through a double layer agar culture system in 26 patients with neutrophilic leucocytosis and compared to the values obtained in 26 normal subjects. Both mean CFU-C incidence and mean leucocyte CSA of the whole group of patients were found significantly higher than normal, but considerable variation was observed among singular patients. The different patterns of blood CFU-C and leucocyte CSA are discussed. The combined evaluation of blood CFU-C and leucocyte CSA is found a useful tool to investigate the pathogenetic mechanisms of neutrophilic leucocytosis.", "contents": "Blood colony-forming cells (CFU-C) and leucocyte colony-stimulating activity (CSA) in patients with neutrophilic leucocytosis. The incidence of circulating colony-forming cells (CFU-C) and the ability of peripheral leucocytes to stimulate the colony formation (CSA) have been studied through a double layer agar culture system in 26 patients with neutrophilic leucocytosis and compared to the values obtained in 26 normal subjects. Both mean CFU-C incidence and mean leucocyte CSA of the whole group of patients were found significantly higher than normal, but considerable variation was observed among singular patients. The different patterns of blood CFU-C and leucocyte CSA are discussed. The combined evaluation of blood CFU-C and leucocyte CSA is found a useful tool to investigate the pathogenetic mechanisms of neutrophilic leucocytosis."}
{"id": "PMID:472656", "title": "Studies of the haemopoietic microenvironment. II. Content of glycosaminoglycans in murine bone marrow and spleen under anaemic and polycythaemic conditions.", "content": "Different glycosaminoglycans are described to be involved in processes of cell proliferation and differentiation. To investigate a possible direct involvement of glycosaminoglycans within the haemopoietic organs in erythropoiesis, biochemical separation and quantification of splenic and bone marrow glycosaminoglycans in anaemic and polycythaemic mice were performed. Hyaluronic acid, chondroitin sulphate A, B and C were present in both organs under both conditions. Both in spleen and bone marrow of polycythaemic mice very low amounts of chondroitin sulphate A, B and C, and a higher amount of hyaluronic acid were found in comparison to normal mice. In anaemic mice only the amount of splenic chondroitin sulphate C was found to be lower than in normal mice. It is demonstrated that considerable changes in sulphated and unsulphated glycosaminoglycans occur during erythropoietic stimulation and suppression. The present findings do not indicate a causal relationship between sulphated glycosaminoglycan levels in the haemopoietic organs and the extend of erythropoietic maturation.", "contents": "Studies of the haemopoietic microenvironment. II. Content of glycosaminoglycans in murine bone marrow and spleen under anaemic and polycythaemic conditions. Different glycosaminoglycans are described to be involved in processes of cell proliferation and differentiation. To investigate a possible direct involvement of glycosaminoglycans within the haemopoietic organs in erythropoiesis, biochemical separation and quantification of splenic and bone marrow glycosaminoglycans in anaemic and polycythaemic mice were performed. Hyaluronic acid, chondroitin sulphate A, B and C were present in both organs under both conditions. Both in spleen and bone marrow of polycythaemic mice very low amounts of chondroitin sulphate A, B and C, and a higher amount of hyaluronic acid were found in comparison to normal mice. In anaemic mice only the amount of splenic chondroitin sulphate C was found to be lower than in normal mice. It is demonstrated that considerable changes in sulphated and unsulphated glycosaminoglycans occur during erythropoietic stimulation and suppression. The present findings do not indicate a causal relationship between sulphated glycosaminoglycan levels in the haemopoietic organs and the extend of erythropoietic maturation."}
{"id": "PMID:472657", "title": "Osteogenesis imperfecta associated with multiple myeloma.", "content": "In a case of osteogenesis imperfecta with multiple fractures already from childhood, myelomatosis was diagnosed at the age of 52 years because of a serum M-component (IgG, lambda), Bence Jones proteinuria, myeloma cells in the bone marrow, and osteolytic skeletal lesions. She died 10 months later. A partial postmortem examination of a larger bone lesion confirmed the diagnosis.", "contents": "Osteogenesis imperfecta associated with multiple myeloma. In a case of osteogenesis imperfecta with multiple fractures already from childhood, myelomatosis was diagnosed at the age of 52 years because of a serum M-component (IgG, lambda), Bence Jones proteinuria, myeloma cells in the bone marrow, and osteolytic skeletal lesions. She died 10 months later. A partial postmortem examination of a larger bone lesion confirmed the diagnosis."}
{"id": "PMID:472658", "title": "Androgen therapy of aplastic anaemia--a prospective study of 352 cases.", "content": "A prospective study of 352 patients with aplastic anaemia on androgen therapy has been performed. The following main observations have been obtained: The actuarial mortality rate at the 20th month is 52%, half the deaths being observed during the first 3 months; these figures are similar to those previously published, from smaller series of androgen-treated patients, and lower than those of non-androgen-treated cases. Differences in survival and improvement were observed between groups of patients treated for more than 3 months with either alkylated or non-alkylated drugs. Signs of liver damage were observed no matter which was the drug used. Continous improvement can be observed even in the 2nd year of treatment indicating that full-dose androgen therapy should be continued up to 20 months in not fully improved patients. The degree of initial disease activity is a clear prognostic parameter for the mortality in the first quarter of the course. In case of survival of severe cases, improvement can be obtained to the same extent as in milder cases. This stress the need for adequate maintenance therapy in all types of patients. Addition of glucocorticoids harms the prognosis, mainly in most granulocytopenic patients. Glucocorticoids have no effect upon the liver damage induced by androgens.", "contents": "Androgen therapy of aplastic anaemia--a prospective study of 352 cases. A prospective study of 352 patients with aplastic anaemia on androgen therapy has been performed. The following main observations have been obtained: The actuarial mortality rate at the 20th month is 52%, half the deaths being observed during the first 3 months; these figures are similar to those previously published, from smaller series of androgen-treated patients, and lower than those of non-androgen-treated cases. Differences in survival and improvement were observed between groups of patients treated for more than 3 months with either alkylated or non-alkylated drugs. Signs of liver damage were observed no matter which was the drug used. Continous improvement can be observed even in the 2nd year of treatment indicating that full-dose androgen therapy should be continued up to 20 months in not fully improved patients. The degree of initial disease activity is a clear prognostic parameter for the mortality in the first quarter of the course. In case of survival of severe cases, improvement can be obtained to the same extent as in milder cases. This stress the need for adequate maintenance therapy in all types of patients. Addition of glucocorticoids harms the prognosis, mainly in most granulocytopenic patients. Glucocorticoids have no effect upon the liver damage induced by androgens."}
{"id": "PMID:472659", "title": "Quantitation of erythropoiesis in myelomatosis.", "content": "Quantitation of the erythropoiesis with radio-iron (59Fe) was applied to 9 patients with untreated myelomatosis. The method included blocking of the 59Fe reutilization by injection of non-radioactive iron. There was no uniform pattern in the Fe-kinetics values. The Plasma Iron Turnover (PIT) and the Red Blood Cell Iron Turnover (RBCIT) varied from subnormal to values markedly increased above upper normal limit. The calculated average Mean Red Cell Life time (MRCL) of erythrocytes was just below normal range. The mean Marrow Transit Time (MTT) was normal in the patients, despite subnormal venous haematocrit, indicating insufficient stimulation of the bone marrow. The renal function, measured as 51Cr-EDTA clearance, was found positively correlated to the RBCIT (r = 0.78, P less than 0.05). The results suggest that the previously demonstrated relationship between anaemia and renal failure in patients with myelomatosis is caused mainly by an inability of the bone marrow to produce sufficient red blood cells under the stress of anaemia related to the degree of renal impairment.", "contents": "Quantitation of erythropoiesis in myelomatosis. Quantitation of the erythropoiesis with radio-iron (59Fe) was applied to 9 patients with untreated myelomatosis. The method included blocking of the 59Fe reutilization by injection of non-radioactive iron. There was no uniform pattern in the Fe-kinetics values. The Plasma Iron Turnover (PIT) and the Red Blood Cell Iron Turnover (RBCIT) varied from subnormal to values markedly increased above upper normal limit. The calculated average Mean Red Cell Life time (MRCL) of erythrocytes was just below normal range. The mean Marrow Transit Time (MTT) was normal in the patients, despite subnormal venous haematocrit, indicating insufficient stimulation of the bone marrow. The renal function, measured as 51Cr-EDTA clearance, was found positively correlated to the RBCIT (r = 0.78, P less than 0.05). The results suggest that the previously demonstrated relationship between anaemia and renal failure in patients with myelomatosis is caused mainly by an inability of the bone marrow to produce sufficient red blood cells under the stress of anaemia related to the degree of renal impairment."}
{"id": "PMID:472660", "title": "Development of human granulocyte and monocyte Fc receptors.", "content": "Bone marrow cells were examined for the presence of IgG-Fc receptors using a red cell rosette technique. Receptors were demonstrated on some of the neutrophil promyelocytes, almost all of the neutrophil myelocytes, and a few of the eosinophil myelocytes. Cells which appeared on morphological grounds to be promonocytes also expressed Fc receptors.", "contents": "Development of human granulocyte and monocyte Fc receptors. Bone marrow cells were examined for the presence of IgG-Fc receptors using a red cell rosette technique. Receptors were demonstrated on some of the neutrophil promyelocytes, almost all of the neutrophil myelocytes, and a few of the eosinophil myelocytes. Cells which appeared on morphological grounds to be promonocytes also expressed Fc receptors."}
{"id": "PMID:472661", "title": "A re-examination of the alleged monocytic features of hairy-cell leukaemia.", "content": "A number of the 'monocytic' features of the hairy cells (HCs) of hairy-cell leukemia (HCL) are studied in detail and discussed in relation to the now established B-cell nature of the disease. By means of a rosette assay employing erythrocytes coated with IgG (EAG), a receptor for the Fc of IgG is shown to be strongly expressed on all HCs, whether in suspension or in monolayers. This receptor is shed and re-expressed over a period of some 6 h. In EAG rosette formation the indicator erythrocytes are deformed and the receptor is not blocked by an antiserum to the Ia-like antigen. By ultrastructural acid-phosphatase cytochemistry, it is established that HCs phagocytose latex particles, but do not phagocytose a variety of other particles to a significant extent. These, and other features, are discussed in relation to the literature and it is shown that all are compatible with the current view that HCL is a form of B-cell lympholiferative.", "contents": "A re-examination of the alleged monocytic features of hairy-cell leukaemia. A number of the 'monocytic' features of the hairy cells (HCs) of hairy-cell leukemia (HCL) are studied in detail and discussed in relation to the now established B-cell nature of the disease. By means of a rosette assay employing erythrocytes coated with IgG (EAG), a receptor for the Fc of IgG is shown to be strongly expressed on all HCs, whether in suspension or in monolayers. This receptor is shed and re-expressed over a period of some 6 h. In EAG rosette formation the indicator erythrocytes are deformed and the receptor is not blocked by an antiserum to the Ia-like antigen. By ultrastructural acid-phosphatase cytochemistry, it is established that HCs phagocytose latex particles, but do not phagocytose a variety of other particles to a significant extent. These, and other features, are discussed in relation to the literature and it is shown that all are compatible with the current view that HCL is a form of B-cell lympholiferative."}
{"id": "PMID:472662", "title": "The varied sensitivity of partial thromboplastin and prothrombin time reagents in the demonstration of the lupus-like anticoagulant.", "content": "An acquired inhibitor of blood coagulation, similar to that described in patients with Systemic Lupus Erythematosus (SLE), was detected during routine coagulation screening in 10 patients who did not meet the criteria for a diagnosis of SLE. The lupus-like anticoagulant (LLAC) was diagnosed on the basis of prolonged activated partial thromboplastin time (APTT) and/or prothrombin time (PT) which failed to correct when patient plasma was added to normal plasma; an additional criterion was an abnormal tissue thromboplastin inhibition test. No patient had a specific inhibitor directed against factors VIII and IX. Demonstration of LLAC was highly dependent upon the type of reagents adopted in the APTT and PT: the abnormality was detected consistently by one reagent only. One-stage assays of factors VIII and IX were characteristic of the presence of an inhibitor, showing non-parellel dose-response curves or decreased activity at low dilutions which were partially corrected at higher dilutions. Although 7 patients were free of abnormal bleeding, unequivocal signs of haemorrhagic tendency after a surgery were present in the remaining 3 patients. The findings suggest that LLAC is a non-exceptional cause of prolonged coagulation screening tests, and that it may sometimes be associated with impaired haemostasis.", "contents": "The varied sensitivity of partial thromboplastin and prothrombin time reagents in the demonstration of the lupus-like anticoagulant. An acquired inhibitor of blood coagulation, similar to that described in patients with Systemic Lupus Erythematosus (SLE), was detected during routine coagulation screening in 10 patients who did not meet the criteria for a diagnosis of SLE. The lupus-like anticoagulant (LLAC) was diagnosed on the basis of prolonged activated partial thromboplastin time (APTT) and/or prothrombin time (PT) which failed to correct when patient plasma was added to normal plasma; an additional criterion was an abnormal tissue thromboplastin inhibition test. No patient had a specific inhibitor directed against factors VIII and IX. Demonstration of LLAC was highly dependent upon the type of reagents adopted in the APTT and PT: the abnormality was detected consistently by one reagent only. One-stage assays of factors VIII and IX were characteristic of the presence of an inhibitor, showing non-parellel dose-response curves or decreased activity at low dilutions which were partially corrected at higher dilutions. Although 7 patients were free of abnormal bleeding, unequivocal signs of haemorrhagic tendency after a surgery were present in the remaining 3 patients. The findings suggest that LLAC is a non-exceptional cause of prolonged coagulation screening tests, and that it may sometimes be associated with impaired haemostasis."}
{"id": "PMID:472663", "title": "Antithrombin III and the nephrotic syndrome.", "content": "Plasma and urinary antithrombin III (AT-III) was measured in 15 cases of nephrotic syndrome. Plasma AT-III correlated well with serum albumin, but poorly with proteinuria, whereas urinary AT-III correlated well to proteinuria. The plasma AT-III level had a mean similar to 25 healthy controls, but the range was significantly wider. A case with nephrotic syndrome and left renal vein thrombosis is reported. The urinary output of AT-III rose and the plasma level fell with the activity of the disease. Although AT-III and albumin have similar molecule weight, their renal clearance was found to be different. It is suggested that urinary loss of AT-III plays a role in the hypercoagulable state sometimes found in the nephrotic syndrome.", "contents": "Antithrombin III and the nephrotic syndrome. Plasma and urinary antithrombin III (AT-III) was measured in 15 cases of nephrotic syndrome. Plasma AT-III correlated well with serum albumin, but poorly with proteinuria, whereas urinary AT-III correlated well to proteinuria. The plasma AT-III level had a mean similar to 25 healthy controls, but the range was significantly wider. A case with nephrotic syndrome and left renal vein thrombosis is reported. The urinary output of AT-III rose and the plasma level fell with the activity of the disease. Although AT-III and albumin have similar molecule weight, their renal clearance was found to be different. It is suggested that urinary loss of AT-III plays a role in the hypercoagulable state sometimes found in the nephrotic syndrome."}
{"id": "PMID:472664", "title": "Impairment of Jones-Mote hypersensitivity and specific antibody response against depolymerized flagellin in lepromatous leprosy.", "content": "Cutaneous hypersensitivity and antibody-producing capacity were assessed in patients with lepromatous leprosy with defective immunity, by immunizing them with monomeric flagellin from Salmonella adelaide. Results were compared with those of controls, matched for age and sex, derived from similar socioeconomic stratum, but without any defect of the immunological system. In contrast to the normal individuals, who showed Jones-Mote type of hypersensitivity, no lepromatous patient could mount any 'delayed-in-time' cutaneous hypersensivivity reaction against an intradermal challenge of monomeric flagellin. However, when immunized through the subcutaneous route, both groups could produce adequate amounts of specific serum antibody. In addition to this unique split tolerance found in all lepromatous patients, some patients showed low levels of 'natural' IgM antibody, reduced formation of specific antibody when immunized through the subcutaneous route, and incomplete maturation of IgG class of anti-flagellin antibody. When immunized by the intradermal route, however, production of both anti-flagellin antibody and maturation of IgG antibody was significantly inhibited in normal adults but not in lepromatous patients. Thus, contrary to the earlier concept of hyperactivity of the humoral immune apparatus in lepromatous leprosy, the present study detected B-cell hypofunction in some patients.", "contents": "Impairment of Jones-Mote hypersensitivity and specific antibody response against depolymerized flagellin in lepromatous leprosy. Cutaneous hypersensitivity and antibody-producing capacity were assessed in patients with lepromatous leprosy with defective immunity, by immunizing them with monomeric flagellin from Salmonella adelaide. Results were compared with those of controls, matched for age and sex, derived from similar socioeconomic stratum, but without any defect of the immunological system. In contrast to the normal individuals, who showed Jones-Mote type of hypersensitivity, no lepromatous patient could mount any 'delayed-in-time' cutaneous hypersensivivity reaction against an intradermal challenge of monomeric flagellin. However, when immunized through the subcutaneous route, both groups could produce adequate amounts of specific serum antibody. In addition to this unique split tolerance found in all lepromatous patients, some patients showed low levels of 'natural' IgM antibody, reduced formation of specific antibody when immunized through the subcutaneous route, and incomplete maturation of IgG class of anti-flagellin antibody. When immunized by the intradermal route, however, production of both anti-flagellin antibody and maturation of IgG antibody was significantly inhibited in normal adults but not in lepromatous patients. Thus, contrary to the earlier concept of hyperactivity of the humoral immune apparatus in lepromatous leprosy, the present study detected B-cell hypofunction in some patients."}
{"id": "PMID:472665", "title": "Demonstration of anti-rubella antibody-secreting cells in rheumatoid arthritis patients.", "content": "In the present study we describe a plaque-forming cell assay using erythrocytes coated with viral antigen, which detected anti-viral antibody-secreting cells against various viral antigens. These anti-viral antibody-secreting cell were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. Rubella anti-viral antibody-secreting cells were present after induction in the peripheral blood of eight out of ten patients. No plaques were seen before induction. Synovial tissue of seven patients out of ten showed rubella-antigen-specific plaques before induction. In all three patients tested, the numbers of plaques increased after induction. The peripheral blood of only one patient showed plaque-forming cells against mumps virus and cytomegalovirus (CMV) antigen. No other patients showed any plaque against CMV, respiratory syncytial virus, mumps virus, measles virus, adenovirus, and varicella zoster virus antigens. The method appears to be promising in studying viral antibody-secreting cells in human immunopathology.", "contents": "Demonstration of anti-rubella antibody-secreting cells in rheumatoid arthritis patients. In the present study we describe a plaque-forming cell assay using erythrocytes coated with viral antigen, which detected anti-viral antibody-secreting cells against various viral antigens. These anti-viral antibody-secreting cell were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. Rubella anti-viral antibody-secreting cells were present after induction in the peripheral blood of eight out of ten patients. No plaques were seen before induction. Synovial tissue of seven patients out of ten showed rubella-antigen-specific plaques before induction. In all three patients tested, the numbers of plaques increased after induction. The peripheral blood of only one patient showed plaque-forming cells against mumps virus and cytomegalovirus (CMV) antigen. No other patients showed any plaque against CMV, respiratory syncytial virus, mumps virus, measles virus, adenovirus, and varicella zoster virus antigens. The method appears to be promising in studying viral antibody-secreting cells in human immunopathology."}
{"id": "PMID:472666", "title": "The amyloid P-component (protein AP): an integral part of the amyloid substance?", "content": "The P-component of amyloid (protein AP) appears to be present in all types of amyloid substance regardless of the clinical category of amyloidosis or the chemical class of the amyloid fibril. The role of protein AP in the formation of amyloid substance has not been established. In a patient with primary amyloidosis, significant amounts of protein AP were found closely associated with the amyloid fibril proteins and was released from the latter only after dissociation and reduction of the amyloid fibril preparation. EDTA seemed to be very effective in releasing protein AP, and it is thought that the close association between the amyloid fibrils and protein AP is calcium-dependent. The very close association between the amyloid fibrils and protein AP suggests that the latter is an integral part of the amyloid substance.", "contents": "The amyloid P-component (protein AP): an integral part of the amyloid substance? The P-component of amyloid (protein AP) appears to be present in all types of amyloid substance regardless of the clinical category of amyloidosis or the chemical class of the amyloid fibril. The role of protein AP in the formation of amyloid substance has not been established. In a patient with primary amyloidosis, significant amounts of protein AP were found closely associated with the amyloid fibril proteins and was released from the latter only after dissociation and reduction of the amyloid fibril preparation. EDTA seemed to be very effective in releasing protein AP, and it is thought that the close association between the amyloid fibrils and protein AP is calcium-dependent. The very close association between the amyloid fibrils and protein AP suggests that the latter is an integral part of the amyloid substance."}
{"id": "PMID:472667", "title": "Paravalvular fistula in mitral valve implant. Intimation of malfunction of implant and quantification of regurgitant flow from effective area measurements.", "content": "Non-invasive ultrasound Doppler registrations and cardiac output determinations with a thermodilution catheter were performed in the presence of and after closure of a paravalvular mitral fistula in a 52-year-old patient with a 27 mm Bj\u00f6rk-Shiley mitral valve. The data collected allowed the calculation of the regurgitant flow through the fistula. This study suggests that the method used can be of diagnostic value when mitral valve implant malfunction is suspected.", "contents": "Paravalvular fistula in mitral valve implant. Intimation of malfunction of implant and quantification of regurgitant flow from effective area measurements. Non-invasive ultrasound Doppler registrations and cardiac output determinations with a thermodilution catheter were performed in the presence of and after closure of a paravalvular mitral fistula in a 52-year-old patient with a 27 mm Bj\u00f6rk-Shiley mitral valve. The data collected allowed the calculation of the regurgitant flow through the fistula. This study suggests that the method used can be of diagnostic value when mitral valve implant malfunction is suspected."}
{"id": "PMID:472668", "title": "Bleeding complications following invasive cardiac studies in anticoagulated patients with artificial heart valves.", "content": "The number and nature of bleeding complications subsequent to cardiac catheterization and angiography were determined in 50 anticoagulated and 50 control patients. There were six bleeding complications in the anticoagulated group and five in the control group. Although the complications were somewhat more severe in anticoagulated patients, the difference was not enough to justify a change in our general policy of continuing anticoagulant treatment in patients with artificial heart valves who are admitted for postoperative invasive cardiac investigations.", "contents": "Bleeding complications following invasive cardiac studies in anticoagulated patients with artificial heart valves. The number and nature of bleeding complications subsequent to cardiac catheterization and angiography were determined in 50 anticoagulated and 50 control patients. There were six bleeding complications in the anticoagulated group and five in the control group. Although the complications were somewhat more severe in anticoagulated patients, the difference was not enough to justify a change in our general policy of continuing anticoagulant treatment in patients with artificial heart valves who are admitted for postoperative invasive cardiac investigations."}
{"id": "PMID:472669", "title": "Congenital heart disease in the first month of life.", "content": "During the years 1963--73, 276 children with congenital heart disease were admitted to this hospital during their first month of life. Ventricular septal defect was the most common cardiac anomaly and this lesion, together with transposition of the great arteries, comprised 35% of all cardiovascular malformations. Extracardiac malformations were found in 86 patients. The cumulative survival rate for all patients was 66% in the first month of life and 33% in the first year. Forty-three patients were operated upon, but it is estimated from necropsy reports and available clinical data that another 74 patients, who died without operation, would have been suitable candidates for total corrective surgery.", "contents": "Congenital heart disease in the first month of life. During the years 1963--73, 276 children with congenital heart disease were admitted to this hospital during their first month of life. Ventricular septal defect was the most common cardiac anomaly and this lesion, together with transposition of the great arteries, comprised 35% of all cardiovascular malformations. Extracardiac malformations were found in 86 patients. The cumulative survival rate for all patients was 66% in the first month of life and 33% in the first year. Forty-three patients were operated upon, but it is estimated from necropsy reports and available clinical data that another 74 patients, who died without operation, would have been suitable candidates for total corrective surgery."}
{"id": "PMID:472670", "title": "Surgical repair of isolated ventricular septal defects in the first year of life.", "content": "Since October 1975, 6 infants ranging in age from 5 to 9 months and weighing from 5.2 to 7.8 kg have been treated with primary closure of ventricular septal defect (VSD) at Ullev\u00e5l Hospital. The indications for operation were large left-to-right shunts combined with persistence of heart failure in 4 patients, a large left-to-right shunt only in one and elevated pulmonary arterial resistance in one patient. Conventional cardiopulmonary bypass was used in all cases. There were no early or late deaths during the mean observation period of 17.3 months (range 3--25 months). One patient developed a recurrent VSD and was successfully re-operated on 8 months after the first operation; otherwise no signs of recurrence were found. The growth and weight gains have been satisfactory and the psychosomatic development of all the infants has been normal. All are in sinus rhythm with right bundle branch block in 4. Cardiac arrhythmias have not been in evidence.", "contents": "Surgical repair of isolated ventricular septal defects in the first year of life. Since October 1975, 6 infants ranging in age from 5 to 9 months and weighing from 5.2 to 7.8 kg have been treated with primary closure of ventricular septal defect (VSD) at Ullev\u00e5l Hospital. The indications for operation were large left-to-right shunts combined with persistence of heart failure in 4 patients, a large left-to-right shunt only in one and elevated pulmonary arterial resistance in one patient. Conventional cardiopulmonary bypass was used in all cases. There were no early or late deaths during the mean observation period of 17.3 months (range 3--25 months). One patient developed a recurrent VSD and was successfully re-operated on 8 months after the first operation; otherwise no signs of recurrence were found. The growth and weight gains have been satisfactory and the psychosomatic development of all the infants has been normal. All are in sinus rhythm with right bundle branch block in 4. Cardiac arrhythmias have not been in evidence."}
{"id": "PMID:472671", "title": "Atrial septal defect of secundum type in patients under 40 years of age. A review of 481 operated cases. Symptoms, signs, treatment and early results.", "content": "In the period 1957--1976 481 patients under 40 years of age were operated on. Dyspnoea and increased fatigue were dominating symptoms in more than half of the series. In 202 cases the anomaly was discovered at routine examination in the absence of relevant symptoms. Almost half of the patients were operated under hypothermia, in the others extracorporeal perfusion was used. Four hospital deaths occurred, two of which were related to cardiopulmonary bypass.", "contents": "Atrial septal defect of secundum type in patients under 40 years of age. A review of 481 operated cases. Symptoms, signs, treatment and early results. In the period 1957--1976 481 patients under 40 years of age were operated on. Dyspnoea and increased fatigue were dominating symptoms in more than half of the series. In 202 cases the anomaly was discovered at routine examination in the absence of relevant symptoms. Almost half of the patients were operated under hypothermia, in the others extracorporeal perfusion was used. Four hospital deaths occurred, two of which were related to cardiopulmonary bypass."}
{"id": "PMID:472672", "title": "Coronary artery spasm--a case with fatal outcome.", "content": "The case of a 45-year-old man with nocturnal angina and angiographically verified spontaneous spasm of the left anterior descending and left circumflex coronary arteries is described. The patient died suddenly one month after investigation, despite treatment with a beta-blocker and nitroglycerin. Other forms of pharmacological treatment should be tried in such patients.", "contents": "Coronary artery spasm--a case with fatal outcome. The case of a 45-year-old man with nocturnal angina and angiographically verified spontaneous spasm of the left anterior descending and left circumflex coronary arteries is described. The patient died suddenly one month after investigation, despite treatment with a beta-blocker and nitroglycerin. Other forms of pharmacological treatment should be tried in such patients."}
{"id": "PMID:472673", "title": "Resting blood pressure index in arterial occlusive disease of the lower limbs.", "content": "The resting blood pressure index of the ankle by the Doppler Ultrasound technique was correlated to the clinical picture and angiographic appearances in 152 legs. A very good correlation existed between the clinical picture and resting pressure index. The latter followed the angiographic status expressed by a score. We could not show any differences between the gropus pains at rest, gangrene or ulceration, either by resting pressure index or by angiographic score. Statistically highly significant differences were found between the resting pressure index and the groups asymptomatic legs, to the intermittent claudication and claudication to pains at rest or gangrene. Irrespective of where the stenosis is localized, the resting blood pressure index of the ankle is the same when the functional status of the patient is the same. This method cannot define the anatomical distribution of occlusive disease; therefore it cannot replace the angiographic examination when there is an indication for reconstructive arterial surgery. However, in the routine assessment, it offers an objective, reproducible and simple method for evaluating the functional effect of occlusive arterial disease in the lower limbs.", "contents": "Resting blood pressure index in arterial occlusive disease of the lower limbs. The resting blood pressure index of the ankle by the Doppler Ultrasound technique was correlated to the clinical picture and angiographic appearances in 152 legs. A very good correlation existed between the clinical picture and resting pressure index. The latter followed the angiographic status expressed by a score. We could not show any differences between the gropus pains at rest, gangrene or ulceration, either by resting pressure index or by angiographic score. Statistically highly significant differences were found between the resting pressure index and the groups asymptomatic legs, to the intermittent claudication and claudication to pains at rest or gangrene. Irrespective of where the stenosis is localized, the resting blood pressure index of the ankle is the same when the functional status of the patient is the same. This method cannot define the anatomical distribution of occlusive disease; therefore it cannot replace the angiographic examination when there is an indication for reconstructive arterial surgery. However, in the routine assessment, it offers an objective, reproducible and simple method for evaluating the functional effect of occlusive arterial disease in the lower limbs."}
{"id": "PMID:472674", "title": "Haemodynamic studies in renal artery stenosis.", "content": "With the aid of an electromagnetic flowmeter and a mechano-electrical pressure transducer, peroperative renal artery blood flows and pressures in the aorta and the renal artery distal to the stenosis were measured in 48 patients with renovascular hypertension before and after renal artery reconstruction. Calculations of pressure difference across the stenosis and resistances over the stenosis and the renal parenchyma were made. There was a significant blood flow increase through the renal artery after arterial reconstruction in all patients, irrespective as to whether they were normotensive, improved or failures postoperatively. The pressure difference across the stenosis after reconstruction was eliminated in all 3 groups of patients. The highest resistances over both the stenosis and the renal parenchyma before arterial reconstruction were found in the normotensive group, but there was no significant variation between the 3 groups. After reconstruction, a significant decrease in resistance over the renal parenchyma was found in the normotensive and the improved group of patients. The resistance of the stenosis was higher than over the renal parenchyma only in the postoperatively normotensive patients. This indicates that the removal of a resistance over the renal artery stenosis, that is higher than that over the renal parenchyma, gives the most favourable long-term results concerning normalization of the blood pressure.", "contents": "Haemodynamic studies in renal artery stenosis. With the aid of an electromagnetic flowmeter and a mechano-electrical pressure transducer, peroperative renal artery blood flows and pressures in the aorta and the renal artery distal to the stenosis were measured in 48 patients with renovascular hypertension before and after renal artery reconstruction. Calculations of pressure difference across the stenosis and resistances over the stenosis and the renal parenchyma were made. There was a significant blood flow increase through the renal artery after arterial reconstruction in all patients, irrespective as to whether they were normotensive, improved or failures postoperatively. The pressure difference across the stenosis after reconstruction was eliminated in all 3 groups of patients. The highest resistances over both the stenosis and the renal parenchyma before arterial reconstruction were found in the normotensive group, but there was no significant variation between the 3 groups. After reconstruction, a significant decrease in resistance over the renal parenchyma was found in the normotensive and the improved group of patients. The resistance of the stenosis was higher than over the renal parenchyma only in the postoperatively normotensive patients. This indicates that the removal of a resistance over the renal artery stenosis, that is higher than that over the renal parenchyma, gives the most favourable long-term results concerning normalization of the blood pressure."}
{"id": "PMID:472675", "title": "Carcinoma arising in a lung cyst.", "content": "Only a few reports on carcinoma arising in lung cysts exist. The literature is reviewed and one new case added, demonstrating the importance of establishing an exact diagnosis by surgery.", "contents": "Carcinoma arising in a lung cyst. Only a few reports on carcinoma arising in lung cysts exist. The literature is reviewed and one new case added, demonstrating the importance of establishing an exact diagnosis by surgery."}
{"id": "PMID:472676", "title": "Multiple pulmonary hamartomas; a case report and review of the literature.", "content": "Bilateral multiple hamartomas were found in a woman suspected of having metastatic malignancy of the lung. As extensive investigation for the primary tumour was unrevealing, a left exploratory thoracotomy and histological examination established the diagnosis. In view of the benign character of the tumours, local excision alone was performed. No surgical intervention was performed on the right side. Repeat chest films 12 months after surgery did not show the appearance of new lesions in the left lung or any increase in the size of the nodules in the right lung. This is the 12th case so far reported. The clinical characteristics and surgical management of these tumours are discussed.", "contents": "Multiple pulmonary hamartomas; a case report and review of the literature. Bilateral multiple hamartomas were found in a woman suspected of having metastatic malignancy of the lung. As extensive investigation for the primary tumour was unrevealing, a left exploratory thoracotomy and histological examination established the diagnosis. In view of the benign character of the tumours, local excision alone was performed. No surgical intervention was performed on the right side. Repeat chest films 12 months after surgery did not show the appearance of new lesions in the left lung or any increase in the size of the nodules in the right lung. This is the 12th case so far reported. The clinical characteristics and surgical management of these tumours are discussed."}
{"id": "PMID:472677", "title": "Chronic spontaneous pneumothorax due solely to a pleurovisceral membrane. A case report.", "content": "The case of a 28-year-old male with a neglected and protracted spontaneous pneumothorax is reported. The condition was diagnosed 45 days after the onset of symptoms. The affected lung had completely collapsed and was unexpandable. Subsequent thoracotomy revealed a firm membrane covering the visceral pleura as the sole explanation of the persistent pulmonary collapse. This variety of chronic pneumothorax seems to be rare. The lung expanded completely after decortication.", "contents": "Chronic spontaneous pneumothorax due solely to a pleurovisceral membrane. A case report. The case of a 28-year-old male with a neglected and protracted spontaneous pneumothorax is reported. The condition was diagnosed 45 days after the onset of symptoms. The affected lung had completely collapsed and was unexpandable. Subsequent thoracotomy revealed a firm membrane covering the visceral pleura as the sole explanation of the persistent pulmonary collapse. This variety of chronic pneumothorax seems to be rare. The lung expanded completely after decortication."}
{"id": "PMID:472678", "title": "Congenital tracheo-oesophageal fistula in the adult. Report of a case and review of the literature.", "content": "Congenital tracheo-oesophageal fistula without atresia can persist and remain undetected until adulthood. We present such a case and give a review of the literature. Symptoms are commonly present during infancy, but to confirm diagnosis of a fistula is often exceedingly difficult. We visualized the fistula in our case by means of a bronchofibrescope when all the other available tests had failed. The speculations as to how these patients reach adult life have been many, but we consider that we have found an important and hitherto undescribed answer to this question, namely a sphincter mechanism in the fistula.", "contents": "Congenital tracheo-oesophageal fistula in the adult. Report of a case and review of the literature. Congenital tracheo-oesophageal fistula without atresia can persist and remain undetected until adulthood. We present such a case and give a review of the literature. Symptoms are commonly present during infancy, but to confirm diagnosis of a fistula is often exceedingly difficult. We visualized the fistula in our case by means of a bronchofibrescope when all the other available tests had failed. The speculations as to how these patients reach adult life have been many, but we consider that we have found an important and hitherto undescribed answer to this question, namely a sphincter mechanism in the fistula."}
{"id": "PMID:472679", "title": "Chronic haemolysis following combined mitral and aortic valve replacement. A randomized study between the Bj\u00f6rk-Shiley and Lillehei-Kaster disc valve prostheses.", "content": "Intravascular haemolysis was studied in a randomized series of 35 patients examined 12--24 months after combined mitral and aortic valve replacements with either the Bj\u00f6rk-Shiley (pyrolytic carbon) tilting disc valve (19 patients) or the Lillehei-Kaster pivoting disc valve (16 patients). Red blood cell count, haemoglobin concentrations, and serum bilirubin varied within normal ranges with a few exceptions. Haptoglobin was absent or reduced in 86%, while elevated values for serum lactate dehydrogenase were found in 82%. The increase in serum lactate dehydrogenase activity was moderate and indicated a normal or only slightly reduced red cell lifespan in most patients. Although the intravascular haemolysis was of little clinical significance, the increase in lactate dehydrogenase was significantly higher in patients with the Lillehei-Kaster prosthesis than in patients with the Bj\u00f6rk-Shiley prosthesis, indicating a slightly shorter lifespan in the former group. For both models, however, intravascular haemolysis was less severe than previously reported after combined aortic and mitral valve replacement with ball valve prosthesis.", "contents": "Chronic haemolysis following combined mitral and aortic valve replacement. A randomized study between the Bj\u00f6rk-Shiley and Lillehei-Kaster disc valve prostheses. Intravascular haemolysis was studied in a randomized series of 35 patients examined 12--24 months after combined mitral and aortic valve replacements with either the Bj\u00f6rk-Shiley (pyrolytic carbon) tilting disc valve (19 patients) or the Lillehei-Kaster pivoting disc valve (16 patients). Red blood cell count, haemoglobin concentrations, and serum bilirubin varied within normal ranges with a few exceptions. Haptoglobin was absent or reduced in 86%, while elevated values for serum lactate dehydrogenase were found in 82%. The increase in serum lactate dehydrogenase activity was moderate and indicated a normal or only slightly reduced red cell lifespan in most patients. Although the intravascular haemolysis was of little clinical significance, the increase in lactate dehydrogenase was significantly higher in patients with the Lillehei-Kaster prosthesis than in patients with the Bj\u00f6rk-Shiley prosthesis, indicating a slightly shorter lifespan in the former group. For both models, however, intravascular haemolysis was less severe than previously reported after combined aortic and mitral valve replacement with ball valve prosthesis."}
{"id": "PMID:472680", "title": "Ten-year mortality from coronary heart disease among 172,000 men classified by occupational physical activity.", "content": "A cohort of 172,489 males aged 20--64 years and employed by the Italian railroad system on 1 April 1963 have been classified by habitual physical activity at work and followed-up for death during a ten-year period. The overall crude mortality was 56.59 per 1,000 in ten years, and no significant differences were found between men in sedentary, moderate and heavy work. Age-corrected death rates for coronary heart disease, as manifested by myocardial infarction and sudden coronary death, were substantially different in the three activity groups, moderately active workers ranking first, sedentary workers second, but very close to the former, and very active workers being last. The age-corrected rates for all ages were 14.18, 12.55 and 7.63 per 1,000 in ten years, respectively. All differences were statistically significant, the mortality ratio between the sedentary and moderate groups combined versus the heavy group being of the order of 1.75 to 1.", "contents": "Ten-year mortality from coronary heart disease among 172,000 men classified by occupational physical activity. A cohort of 172,489 males aged 20--64 years and employed by the Italian railroad system on 1 April 1963 have been classified by habitual physical activity at work and followed-up for death during a ten-year period. The overall crude mortality was 56.59 per 1,000 in ten years, and no significant differences were found between men in sedentary, moderate and heavy work. Age-corrected death rates for coronary heart disease, as manifested by myocardial infarction and sudden coronary death, were substantially different in the three activity groups, moderately active workers ranking first, sedentary workers second, but very close to the former, and very active workers being last. The age-corrected rates for all ages were 14.18, 12.55 and 7.63 per 1,000 in ten years, respectively. All differences were statistically significant, the mortality ratio between the sedentary and moderate groups combined versus the heavy group being of the order of 1.75 to 1."}
{"id": "PMID:472681", "title": "Ten-year coronary mortality of workers exposed to carbon disulfide.", "content": "Two cohorts, one comprising 343 viscose rayon workers exposed for at least five years to carbon disulfide (CS2) and the other made up of 343 nonexposed men, were followed from 1967 to 1977 with respect to coronary heart disease (CHD) mortality. In the examination in 1967 known risk factors of CHD were controlled; only blood pressure was slightly higher in the exposed group. The difference was considered a result of exposure. Five exposed and eight nonexposed men had experienced a previous clinical myocardial infarction. The total mortality was 48 (14%) in the exposed group and 31 (9%) in the compeer group (p congruent to 0.05); 29 exposed and 11 nonexposed men had died from CHD (p less than 0.01), and 5 exposed and 1 nonexposed from other cardiovascular causes (p = 0.1). All coronary deaths occurred in the age interval 40 to 69 years. The estimated risk of death from CHD for this 30-year age span, assuming no competing risks of death, was 31.9% for the exposed cohort against 13.3% for the compeers. A multivariate analysis yielded age, raised blood pressure, and exposure to CS2 as prominent risk factors. The contributory risk caused by past occupational CS2 exposure seemed to increase the already notoriously high risk of CHD mortality among Finnish men.", "contents": "Ten-year coronary mortality of workers exposed to carbon disulfide. Two cohorts, one comprising 343 viscose rayon workers exposed for at least five years to carbon disulfide (CS2) and the other made up of 343 nonexposed men, were followed from 1967 to 1977 with respect to coronary heart disease (CHD) mortality. In the examination in 1967 known risk factors of CHD were controlled; only blood pressure was slightly higher in the exposed group. The difference was considered a result of exposure. Five exposed and eight nonexposed men had experienced a previous clinical myocardial infarction. The total mortality was 48 (14%) in the exposed group and 31 (9%) in the compeer group (p congruent to 0.05); 29 exposed and 11 nonexposed men had died from CHD (p less than 0.01), and 5 exposed and 1 nonexposed from other cardiovascular causes (p = 0.1). All coronary deaths occurred in the age interval 40 to 69 years. The estimated risk of death from CHD for this 30-year age span, assuming no competing risks of death, was 31.9% for the exposed cohort against 13.3% for the compeers. A multivariate analysis yielded age, raised blood pressure, and exposure to CS2 as prominent risk factors. The contributory risk caused by past occupational CS2 exposure seemed to increase the already notoriously high risk of CHD mortality among Finnish men."}
{"id": "PMID:472682", "title": "Long-term exposure to electric fields. A cross-sectional epidemiologic investigation of occupationally exposed workers in high-voltage substations.", "content": "In the present epidemiologic study, 53 workers with a long-term (more than five years) exposure to the electric field of 400 kV substations were examined and compared with a matched reference group of 53 nonexposed workers from the same power companies. Matching variables included age, geographic location and employment time. The aim of the study was to investigate the possibility of persistent, chronic health effects in the exposed group as a consequence of exposure. The investigation indluded the nervous system (neurasthenic symptoms, psychological tests, electroencephalography), the cardiovascular system (symptoms, blood pressure, electrocardiography), and the blood (hemoglobin, red blood cells, reticulocytes, white blood cells including differential count, thrombocytes, sedimentation rate). Fertility was also assessed. The results showed no differences between the exposed and reference groups as a consequence of the long-term exposure to the electric fields. The groups differed, however, in that the exposed group had (a) consistently better results on the psychological performance tests, (b) a fewer number of children, especially boys, and (c) somewhat higher education. The differences in test results were due to the higher education among the exposed. The difference in number of children was also thought to be related to factors other than exposure since it was found to be present already 10--15 years before the work in 400 kV substations began.", "contents": "Long-term exposure to electric fields. A cross-sectional epidemiologic investigation of occupationally exposed workers in high-voltage substations. In the present epidemiologic study, 53 workers with a long-term (more than five years) exposure to the electric field of 400 kV substations were examined and compared with a matched reference group of 53 nonexposed workers from the same power companies. Matching variables included age, geographic location and employment time. The aim of the study was to investigate the possibility of persistent, chronic health effects in the exposed group as a consequence of exposure. The investigation indluded the nervous system (neurasthenic symptoms, psychological tests, electroencephalography), the cardiovascular system (symptoms, blood pressure, electrocardiography), and the blood (hemoglobin, red blood cells, reticulocytes, white blood cells including differential count, thrombocytes, sedimentation rate). Fertility was also assessed. The results showed no differences between the exposed and reference groups as a consequence of the long-term exposure to the electric fields. The groups differed, however, in that the exposed group had (a) consistently better results on the psychological performance tests, (b) a fewer number of children, especially boys, and (c) somewhat higher education. The differences in test results were due to the higher education among the exposed. The difference in number of children was also thought to be related to factors other than exposure since it was found to be present already 10--15 years before the work in 400 kV substations began."}
{"id": "PMID:472683", "title": "Distribution of m-xylene to subcutaneous adipose tissue in short-term experimental human exposure.", "content": "When volunteers were exposed in three different patterns of exposure to approximately 3.7--8.2 mumol/l (90--200 ppm, constant or time-weighted average concentration) of m-xylene for five consecutive days plus one day after the weekend, approximately 3.7--8.0% of the total body uptake was estimated to be distributed to the adipose tissue on the basis of m-xylene determinations made from subcutaneous fat. Distribution seemed to be affected by the exposure pattern; a proportionately greater distribution to subcutaneous fat occurred when the volunteers performed physical exercise part of the time, particularly when the peak xylene concentrations in the air coincided with the exercise. The median elimination half-time of m-xylene from subcutaneous fat was 58 h (range 25--128 h). A positive correlation was found between the individual elimination half-times of m-xylene in subcutaneous fat and the relative fat mass of the individuals in two exposure groups, but not in one.", "contents": "Distribution of m-xylene to subcutaneous adipose tissue in short-term experimental human exposure. When volunteers were exposed in three different patterns of exposure to approximately 3.7--8.2 mumol/l (90--200 ppm, constant or time-weighted average concentration) of m-xylene for five consecutive days plus one day after the weekend, approximately 3.7--8.0% of the total body uptake was estimated to be distributed to the adipose tissue on the basis of m-xylene determinations made from subcutaneous fat. Distribution seemed to be affected by the exposure pattern; a proportionately greater distribution to subcutaneous fat occurred when the volunteers performed physical exercise part of the time, particularly when the peak xylene concentrations in the air coincided with the exercise. The median elimination half-time of m-xylene from subcutaneous fat was 58 h (range 25--128 h). A positive correlation was found between the individual elimination half-times of m-xylene in subcutaneous fat and the relative fat mass of the individuals in two exposure groups, but not in one."}
{"id": "PMID:472686", "title": "Determination of inorganic fiber density in human lung tissue by scanning electron microscopy after low temperature ashing.", "content": "The concentration of asbestos fibers in lung tissue may give information about previous occupational asbestos exposure. A procedure for the preparation, quantification and identification of asbestos fibers in human tissue is presented in this report. The procedure is based on low-temperature plasma ashing of dry tissue, examination of the ash by scanning electron microscopy, and energy dispersive X-ray microanalysis. The advantages and disadvantages of this method are also discussed briefly.", "contents": "Determination of inorganic fiber density in human lung tissue by scanning electron microscopy after low temperature ashing. The concentration of asbestos fibers in lung tissue may give information about previous occupational asbestos exposure. A procedure for the preparation, quantification and identification of asbestos fibers in human tissue is presented in this report. The procedure is based on low-temperature plasma ashing of dry tissue, examination of the ash by scanning electron microscopy, and energy dispersive X-ray microanalysis. The advantages and disadvantages of this method are also discussed briefly."}
{"id": "PMID:472687", "title": "Styrene and styrene oxide concentrations in the air during the lamination process in the reinforced plastics industry.", "content": "Styrene and styrene oxide concentrations were measured during the lamination process in the reinforced plastics industry. The mean concentration of styrene in the personal samples was 130 ppm, the highest value measured being 350 ppm. The average concentration for styrene oxide alone was 0.1 ppm, whereas the corresponding measurement for styrene oxide and its decomposition products combined was 0.7 ppm. In comparison then, the concentrations of styrene oxide and its derivatives were much lower (about 0.5% of the total) than those of styrene.", "contents": "Styrene and styrene oxide concentrations in the air during the lamination process in the reinforced plastics industry. Styrene and styrene oxide concentrations were measured during the lamination process in the reinforced plastics industry. The mean concentration of styrene in the personal samples was 130 ppm, the highest value measured being 350 ppm. The average concentration for styrene oxide alone was 0.1 ppm, whereas the corresponding measurement for styrene oxide and its decomposition products combined was 0.7 ppm. In comparison then, the concentrations of styrene oxide and its derivatives were much lower (about 0.5% of the total) than those of styrene."}
{"id": "PMID:472690", "title": "[New methods of radiologic diagnosis: ultrasonography and tomoradiometry (computer assisted tomography)].", "content": "The evolution of new and more accurate methods of examination is a welcome complement to current diagnostic resources. However, it is essential to use them intelligently to avoid a further undesirable rise in the cost of health. Now more than ever, the programme of radiologic examinations should be established by common consent between the practising physician and the consultant radiologist.", "contents": "[New methods of radiologic diagnosis: ultrasonography and tomoradiometry (computer assisted tomography)]. The evolution of new and more accurate methods of examination is a welcome complement to current diagnostic resources. However, it is essential to use them intelligently to avoid a further undesirable rise in the cost of health. Now more than ever, the programme of radiologic examinations should be established by common consent between the practising physician and the consultant radiologist."}
{"id": "PMID:472691", "title": "[Echocardiography. Advantages and limitations related to unidimensional and bidimensional technics in real time].", "content": "Mode-M echocardiography is the most valuable non invasive technique in cardiology, but as a one-dimensional technique it displays the cardiac structure in an unfamiliar form without resemblance to the cardiac anatomy. Two-dimensional ultrasonic imaging of the heart overcomes this disadvantage. However, the definition of these scanners leaves much to be desired and becomes very poor when the stop-frame mode is used. The major disadvantages of the \"multiscan\" are the length of the multicrystal transducer, distortion of the picture, spurious echoes and verticalization. The sector scanner has smaller transducers fitting the \"echocardiographic window\" and permitting an apical, subxiphoid and suprasternal approach. However, with this system resolution is usually poor in the first anterior 3.5--4 cm after the thoracic wall. The mechanical scanner causes a vibrating sensation occasionally irritating to the patient. The electronic sector scanner has the widest angle and provides the best quality images. Unfortunately, its cost is markedly higher than that of other two-dimensional systems.", "contents": "[Echocardiography. Advantages and limitations related to unidimensional and bidimensional technics in real time]. Mode-M echocardiography is the most valuable non invasive technique in cardiology, but as a one-dimensional technique it displays the cardiac structure in an unfamiliar form without resemblance to the cardiac anatomy. Two-dimensional ultrasonic imaging of the heart overcomes this disadvantage. However, the definition of these scanners leaves much to be desired and becomes very poor when the stop-frame mode is used. The major disadvantages of the \"multiscan\" are the length of the multicrystal transducer, distortion of the picture, spurious echoes and verticalization. The sector scanner has smaller transducers fitting the \"echocardiographic window\" and permitting an apical, subxiphoid and suprasternal approach. However, with this system resolution is usually poor in the first anterior 3.5--4 cm after the thoracic wall. The mechanical scanner causes a vibrating sensation occasionally irritating to the patient. The electronic sector scanner has the widest angle and provides the best quality images. Unfortunately, its cost is markedly higher than that of other two-dimensional systems."}
{"id": "PMID:472692", "title": "[Possibilities of abdominal ultrasonics].", "content": "The potentialities of abdominal ultrasonography are discussed and illustrated by a number of cases. It is particularly indicated whenever aortic aneurysm, gallstones or obstructive jaundice are suspected. However, good results are obtained in research of most forms of abdominal mass lesion.", "contents": "[Possibilities of abdominal ultrasonics]. The potentialities of abdominal ultrasonography are discussed and illustrated by a number of cases. It is particularly indicated whenever aortic aneurysm, gallstones or obstructive jaundice are suspected. However, good results are obtained in research of most forms of abdominal mass lesion."}
{"id": "PMID:472693", "title": "[Abdominal tomoradiometry: its possibilities].", "content": "Computer-assisted tomography opens up a new diagnostic chapter in research on abdominal lesions. The display of very faint contrasts, and the possibility of modulating them and of strengthening them by opacifying agents, afford hitherto unknown differentiations. The obtaining of cross-sections is another advantage of this method. Its use for study of the various abdominal organs is reviewed.", "contents": "[Abdominal tomoradiometry: its possibilities]. Computer-assisted tomography opens up a new diagnostic chapter in research on abdominal lesions. The display of very faint contrasts, and the possibility of modulating them and of strengthening them by opacifying agents, afford hitherto unknown differentiations. The obtaining of cross-sections is another advantage of this method. Its use for study of the various abdominal organs is reviewed."}
{"id": "PMID:472694", "title": "[Computer-assisted tomography of the chest].", "content": "Computer-assisted tomography of the chest is the most accurate technique in diagnostic imaging of tumoral disease of the mediastinum. It is also a reliable method of detecting lymphadenopathies and lung metastases, as well as any pathological process involving the pleura and the thoracic wall.", "contents": "[Computer-assisted tomography of the chest]. Computer-assisted tomography of the chest is the most accurate technique in diagnostic imaging of tumoral disease of the mediastinum. It is also a reliable method of detecting lymphadenopathies and lung metastases, as well as any pathological process involving the pleura and the thoracic wall."}
{"id": "PMID:472697", "title": "[Use of the determination of \"fast\" hemoglobins for surveillance and diagnosis of diabetes mellitus].", "content": "Personal results in the assay of \"fast hemoglobins\" in diabetics and normal controls are reported. For the latter, a normal range of 5.45--8.25% HbA1 has been determined. Correlation between HbA1 and fasting blood sugar was found for diabetics, the correlation being at its highest for a group of diabetics with rather constant fasting blood sugar. From the results of this group it proved possible to calculate a simple system for the evaluation of therapy. The diagnostic of HbA1 is limited, since concentrations remain normal in mild diabetics with normal fasting blood sugar.", "contents": "[Use of the determination of \"fast\" hemoglobins for surveillance and diagnosis of diabetes mellitus]. Personal results in the assay of \"fast hemoglobins\" in diabetics and normal controls are reported. For the latter, a normal range of 5.45--8.25% HbA1 has been determined. Correlation between HbA1 and fasting blood sugar was found for diabetics, the correlation being at its highest for a group of diabetics with rather constant fasting blood sugar. From the results of this group it proved possible to calculate a simple system for the evaluation of therapy. The diagnostic of HbA1 is limited, since concentrations remain normal in mild diabetics with normal fasting blood sugar."}
{"id": "PMID:472699", "title": "[Control of oral anticoagulation: comparison between Quick and colorimetric factor X determination in 107 patients].", "content": "Since monitoring of oral anticoagulation (OA) by prothrombin time (PT) is a source of standardization difficulties, the authors have tested another approach. 107 patients under long term OA were monitored by both PT and a colorimetric factor X assay (in vitro activation of factor X with RVV and assessment of amidolytic activity towards S-2222). The PT values were between 10 and 34% (therapeutic range 15--25%), and factor X levels were between 10 and 44% (therapeutic range 16--24%). The correlation between the two methods was highly significant (r = 0.65, p less than 0.001). In 68% of the patients the two tests gave the same information (55 subjects were adequately, 16 insufficiently and 2 over-anticoagulated). For a slightly broader therapeutic range (PT 15--30%, corresponding to 16--28% factor X) concordant information was obtained in 82% of the patients. During stable OA, PT and factor X assay gave very similar information. An advantage of the latter method is the possibility of automation, while a disadvantage is its insensitivity to factor VII.", "contents": "[Control of oral anticoagulation: comparison between Quick and colorimetric factor X determination in 107 patients]. Since monitoring of oral anticoagulation (OA) by prothrombin time (PT) is a source of standardization difficulties, the authors have tested another approach. 107 patients under long term OA were monitored by both PT and a colorimetric factor X assay (in vitro activation of factor X with RVV and assessment of amidolytic activity towards S-2222). The PT values were between 10 and 34% (therapeutic range 15--25%), and factor X levels were between 10 and 44% (therapeutic range 16--24%). The correlation between the two methods was highly significant (r = 0.65, p less than 0.001). In 68% of the patients the two tests gave the same information (55 subjects were adequately, 16 insufficiently and 2 over-anticoagulated). For a slightly broader therapeutic range (PT 15--30%, corresponding to 16--28% factor X) concordant information was obtained in 82% of the patients. During stable OA, PT and factor X assay gave very similar information. An advantage of the latter method is the possibility of automation, while a disadvantage is its insensitivity to factor VII."}
{"id": "PMID:472702", "title": "[Procetophen--an alternative in the treatment of hypercholsterinemia?].", "content": "18 patients with primary hyperlipoproteinemia of type IIa and type IIb, on regularly controlled dietary treatment for 6 months and a 4 weeks placebo period, were given 300 mg procetofene per day for 12 weeks. The subjective tolerance of the drug was good. The serum triglyceride levels were lowered by about 35%, total cholesterol by 15%, LDL-cholesterol by 12% and Apo B by 20%. The HDL-cholesterol values remained unchanged. A slight increase in SGPT values indicates that liver function should be regularly checked in patients undergoing procetofene therapy.", "contents": "[Procetophen--an alternative in the treatment of hypercholsterinemia?]. 18 patients with primary hyperlipoproteinemia of type IIa and type IIb, on regularly controlled dietary treatment for 6 months and a 4 weeks placebo period, were given 300 mg procetofene per day for 12 weeks. The subjective tolerance of the drug was good. The serum triglyceride levels were lowered by about 35%, total cholesterol by 15%, LDL-cholesterol by 12% and Apo B by 20%. The HDL-cholesterol values remained unchanged. A slight increase in SGPT values indicates that liver function should be regularly checked in patients undergoing procetofene therapy."}
{"id": "PMID:472703", "title": "[Popliteal cysts: unusual form of presentation].", "content": "Popliteal cysts are usually associated with abnormalities of the knee joint such as osteoarthritis, chronic inflammation or cartilage tears. 15 patients with popliteal cysts demonstrated by arthrography are described who had no obvious lesion of the knee. They had consulted their physician for symptoms without a clearcut relation to the knee joint, such as tenderness on the external side of the calf, ankle edema or paresthesia in the toes. Standard X-rays and clinical examination of the knee were normal except for tender swelling of the popliteal area. The symptoms improved after injection of triamcinolone into the knee joint. Unexplained symptoms of the lower leg may be due to popliteal cysts even in the absence of obvious knee pathology.", "contents": "[Popliteal cysts: unusual form of presentation]. Popliteal cysts are usually associated with abnormalities of the knee joint such as osteoarthritis, chronic inflammation or cartilage tears. 15 patients with popliteal cysts demonstrated by arthrography are described who had no obvious lesion of the knee. They had consulted their physician for symptoms without a clearcut relation to the knee joint, such as tenderness on the external side of the calf, ankle edema or paresthesia in the toes. Standard X-rays and clinical examination of the knee were normal except for tender swelling of the popliteal area. The symptoms improved after injection of triamcinolone into the knee joint. Unexplained symptoms of the lower leg may be due to popliteal cysts even in the absence of obvious knee pathology."}
{"id": "PMID:472704", "title": "[Endocrinologic and ophthalmologic findings before and after transnasal surgery in so-called chromophobe pituitary adenomas].", "content": "Pre- and postoperative visual and endocrine functions were examined in 54 patients with so-called chromophobe pituitary adenomas operated on by the transnasal approach. The preoperative examination showed visual disturbances in 41 of 48 patients. A loss of gonadal function was present in 93% of the women and 69% of the men. Impairment of adrenal function was found in 37% and of thyroid function in 18% of patients. Hyperprolactinemia was present in 16 of 29 patients examined. The visual findings remained unchanged, normal or improved in 75% of patients. Impairment occurred in 3%. Eight patients experienced improvement of gonadal function and 3 of adrenal function, whereas no postoperative improvement of the thyroid function was observed. Deterioration of gonadal function occurred in 4, of adrenal function in 7, and of thyroid function in 9 patients. Hyperprolactinemia was reduced in all cases.", "contents": "[Endocrinologic and ophthalmologic findings before and after transnasal surgery in so-called chromophobe pituitary adenomas]. Pre- and postoperative visual and endocrine functions were examined in 54 patients with so-called chromophobe pituitary adenomas operated on by the transnasal approach. The preoperative examination showed visual disturbances in 41 of 48 patients. A loss of gonadal function was present in 93% of the women and 69% of the men. Impairment of adrenal function was found in 37% and of thyroid function in 18% of patients. Hyperprolactinemia was present in 16 of 29 patients examined. The visual findings remained unchanged, normal or improved in 75% of patients. Impairment occurred in 3%. Eight patients experienced improvement of gonadal function and 3 of adrenal function, whereas no postoperative improvement of the thyroid function was observed. Deterioration of gonadal function occurred in 4, of adrenal function in 7, and of thyroid function in 9 patients. Hyperprolactinemia was reduced in all cases."}
{"id": "PMID:472705", "title": "[Experiences with an oral cocaine-morphine solution (Brompton mixture) in the treatment of severe pain in patients with neoplasms].", "content": "One year's experience is reported in the treatment of terminally ill cancer patients with an oral solution of morphine and cocaine on a fixed schedule. 36 consecutive patients were treated for an average of 51 days. Excellent or good pain relief was achieved with 26 patients (72%). The initial mean daily dose of morphine was 35 mg. After 12 weeks, the dose had to be raised to an average of 52 mg per day. Some patients received up to 180 mg morphine per day. Side effects were not uncommon but were without clinical consequences in most patients. Side effects caused discontinuation of treatment in only 2 patients. Terminally ill cancer patients often derive great benefit from this treatment.", "contents": "[Experiences with an oral cocaine-morphine solution (Brompton mixture) in the treatment of severe pain in patients with neoplasms]. One year's experience is reported in the treatment of terminally ill cancer patients with an oral solution of morphine and cocaine on a fixed schedule. 36 consecutive patients were treated for an average of 51 days. Excellent or good pain relief was achieved with 26 patients (72%). The initial mean daily dose of morphine was 35 mg. After 12 weeks, the dose had to be raised to an average of 52 mg per day. Some patients received up to 180 mg morphine per day. Side effects were not uncommon but were without clinical consequences in most patients. Side effects caused discontinuation of treatment in only 2 patients. Terminally ill cancer patients often derive great benefit from this treatment."}
{"id": "PMID:472708", "title": "Isolation and partial characterization of a water-soluble receptor protein of insulin.", "content": "By using the chelate EDTA at low concentration to remove the bivalent cations in the plasma membranes and followed by n-butanol to extract the membrane lipids, we have obtained a water-soluble insulin receptor without detergent from the liver-cell plasma membranes. This receptor protein does not precipitate by centrifugation at 300,000 X g for 70 min, nor does it retain on 0.22 micron millipore filter. It does not retard on Sephadex G-200 gel chromatographic column either. It is thus proved that the insulin-receptor protein obtained by this method is completely soluble in water. The dissociation constant of the water-soluble receptor of insulin at 24 degrees C is calculated to be 3.6 X 10(-9) M and its isoelectric point was approximately pH 4.1. As the fluorescence hydrophobic probe 1,8-ANS does not significantly affect the binding activity between the receptor and the insulin, it seems that in addition to a hydrophobic binding mechanism, there may exist some other forces of interaction.", "contents": "Isolation and partial characterization of a water-soluble receptor protein of insulin. By using the chelate EDTA at low concentration to remove the bivalent cations in the plasma membranes and followed by n-butanol to extract the membrane lipids, we have obtained a water-soluble insulin receptor without detergent from the liver-cell plasma membranes. This receptor protein does not precipitate by centrifugation at 300,000 X g for 70 min, nor does it retain on 0.22 micron millipore filter. It does not retard on Sephadex G-200 gel chromatographic column either. It is thus proved that the insulin-receptor protein obtained by this method is completely soluble in water. The dissociation constant of the water-soluble receptor of insulin at 24 degrees C is calculated to be 3.6 X 10(-9) M and its isoelectric point was approximately pH 4.1. As the fluorescence hydrophobic probe 1,8-ANS does not significantly affect the binding activity between the receptor and the insulin, it seems that in addition to a hydrophobic binding mechanism, there may exist some other forces of interaction."}
{"id": "PMID:472709", "title": "Viroids: structure and function.", "content": "Viroids are nucleic acid species of relatively low molecular weight and unique structure that cause several important diseases of cultivated plants. Similar nucleic acid species may be responsible for certain diseases of animals and humans. Viroids are the smallest known agents of infectious disease. Unlike viral nucleic acids, viroids are not encapsidated. Despite their small size, viroids replicate autonomously in cells of susceptible plant species. Known viroids are single-stranded, covalently closed circular, as well as linear, RNA molecules with extensive regions of intramolecular complementarity; they exist in their native state as highly base-paired rods.", "contents": "Viroids: structure and function. Viroids are nucleic acid species of relatively low molecular weight and unique structure that cause several important diseases of cultivated plants. Similar nucleic acid species may be responsible for certain diseases of animals and humans. Viroids are the smallest known agents of infectious disease. Unlike viral nucleic acids, viroids are not encapsidated. Despite their small size, viroids replicate autonomously in cells of susceptible plant species. Known viroids are single-stranded, covalently closed circular, as well as linear, RNA molecules with extensive regions of intramolecular complementarity; they exist in their native state as highly base-paired rods."}
{"id": "PMID:472711", "title": "Genes coding for histone proteins in man are located on the distal end of the long arm of chromosome 7.", "content": "Tritium-labeled complementary RNA's to two cloned sea urchin DNA sequences, one coding for histones H1, H2B, and H4 and the other for H2A and H3, were hybridized in situ to high resolution human chromosomes. Evidence is presented showing that the histone genes in man are localized in bands q32-36 on the long arm of chromosome 7.", "contents": "Genes coding for histone proteins in man are located on the distal end of the long arm of chromosome 7. Tritium-labeled complementary RNA's to two cloned sea urchin DNA sequences, one coding for histones H1, H2B, and H4 and the other for H2A and H3, were hybridized in situ to high resolution human chromosomes. Evidence is presented showing that the histone genes in man are localized in bands q32-36 on the long arm of chromosome 7."}
{"id": "PMID:472712", "title": "M\u00fcllerian duct regression in the embryo correlated with cytotoxic activity against human ovarian cancer.", "content": "A significant cytotoxicity index was obtained when human ovarian cancer cells in a microcytotoxicity assay were exposed during the S (DNA-synthesizing) phase of the cell cycle to purified fractions of testis exhibiting high M\u00fcllerian inhibiting substance bioactivity. The same effect was not observed when these fractions were tested against human glioblastoma or fibroblast lines. Most human ovarian cancers are said to resemble M\u00fcllerian tissues histologically. M\u00fcllerian inhibiting substance may thus deserve further study as a potential chemotherapeutic agent.", "contents": "M\u00fcllerian duct regression in the embryo correlated with cytotoxic activity against human ovarian cancer. A significant cytotoxicity index was obtained when human ovarian cancer cells in a microcytotoxicity assay were exposed during the S (DNA-synthesizing) phase of the cell cycle to purified fractions of testis exhibiting high M\u00fcllerian inhibiting substance bioactivity. The same effect was not observed when these fractions were tested against human glioblastoma or fibroblast lines. Most human ovarian cancers are said to resemble M\u00fcllerian tissues histologically. M\u00fcllerian inhibiting substance may thus deserve further study as a potential chemotherapeutic agent."}
{"id": "PMID:472713", "title": "Vascular smooth muscle cell kinetics: a new assay for studying patterns of cellular proliferation in vivo.", "content": "A new quantitative assay for studying the kinetics of vascular smooth muscle cells in vivo is reported. The assay was used to determine the specific activity of DNA from rabbit aortic smooth muscle cells stimulated to grow by removal of the endothelial layer. The specific activity of the DNA was correlated with the rate of tritiated thymidine incorporation as measured by autoradiography and with the rate of DNA synthesis as estimated by direct measurement of cellular proliferation. Smooth muscle cells exhibit a 24-hour latent period in vivo prior to DNA synthesis; the synthesis peaks at 48 hours and then rapidly declines. The decline in DNA synthesis is not related to endothelial regrowth, and may be of homeostatic significance in limiting luminal stenosis. The assay offers a rapid and reliable alternative to autoradiographic and morphometric techniques for evaluating growth kinetics and growth regulation in vivo.", "contents": "Vascular smooth muscle cell kinetics: a new assay for studying patterns of cellular proliferation in vivo. A new quantitative assay for studying the kinetics of vascular smooth muscle cells in vivo is reported. The assay was used to determine the specific activity of DNA from rabbit aortic smooth muscle cells stimulated to grow by removal of the endothelial layer. The specific activity of the DNA was correlated with the rate of tritiated thymidine incorporation as measured by autoradiography and with the rate of DNA synthesis as estimated by direct measurement of cellular proliferation. Smooth muscle cells exhibit a 24-hour latent period in vivo prior to DNA synthesis; the synthesis peaks at 48 hours and then rapidly declines. The decline in DNA synthesis is not related to endothelial regrowth, and may be of homeostatic significance in limiting luminal stenosis. The assay offers a rapid and reliable alternative to autoradiographic and morphometric techniques for evaluating growth kinetics and growth regulation in vivo."}
{"id": "PMID:472714", "title": "Memory load and event rate control sensitivity decrements in sustained attention.", "content": "The capacity to sustain attention at an efficient level deteriorates over time in discrimination and monitoring tasks. This \"vigilance decrement\" results from a decrement in perceptual sensitivity only if (i) target discrimination loads memory and (ii) stimulus events occur rapidly; otherwise, the decrement reflects temporal changes in response criteria. These results provide a basis for distinguishing between the perceptual and response processes underlying the vigilance decrement that may be generalized across a range of tasks.", "contents": "Memory load and event rate control sensitivity decrements in sustained attention. The capacity to sustain attention at an efficient level deteriorates over time in discrimination and monitoring tasks. This \"vigilance decrement\" results from a decrement in perceptual sensitivity only if (i) target discrimination loads memory and (ii) stimulus events occur rapidly; otherwise, the decrement reflects temporal changes in response criteria. These results provide a basis for distinguishing between the perceptual and response processes underlying the vigilance decrement that may be generalized across a range of tasks."}
{"id": "PMID:472715", "title": "Emergence of interoceptive and exteroceptive control of behavior in rats.", "content": "The role of exteroceptive and interoceptive aversive stimuli in rats 2 to 14 days old was investigated according to an odor aversion paradigm. Amyl acetate odor was paired with eigher peripheral shock, intraperitoneal shock, or lithium chloride poisoning. Intraperitoneal shock was an effective unconditioned stimulus at all ages and produced odor aversions comparable to lithium chloride poisoning; peripheral shock, however, was effective only in rats 10 days of age or older. Interoceptive control of aversively motivated behaviors thus seems to develop before exteroceptive control, and the failure of previous studies to find reliable learning and retention of shock-motivated behaviors before 8 to 10 days of age may be attributable to the site to which shock was applied rather than to insensitivity to shock per se.", "contents": "Emergence of interoceptive and exteroceptive control of behavior in rats. The role of exteroceptive and interoceptive aversive stimuli in rats 2 to 14 days old was investigated according to an odor aversion paradigm. Amyl acetate odor was paired with eigher peripheral shock, intraperitoneal shock, or lithium chloride poisoning. Intraperitoneal shock was an effective unconditioned stimulus at all ages and produced odor aversions comparable to lithium chloride poisoning; peripheral shock, however, was effective only in rats 10 days of age or older. Interoceptive control of aversively motivated behaviors thus seems to develop before exteroceptive control, and the failure of previous studies to find reliable learning and retention of shock-motivated behaviors before 8 to 10 days of age may be attributable to the site to which shock was applied rather than to insensitivity to shock per se."}
{"id": "PMID:472716", "title": "Lithium transport across red cell membrane: a cell membrane abnormality in manic-depressive illness.", "content": "In the families of manic-depressive patients, relatives with a history of affective disorders had a significantly higher ratio of mean red cell lithium to plasma lithium in vitro than relatives with no such history. A genetically controlled abnormality in lithium-sodium transport, the mechanism that determines the lithium ratio, may play a role in the etiology of some forms of affective disorders.", "contents": "Lithium transport across red cell membrane: a cell membrane abnormality in manic-depressive illness. In the families of manic-depressive patients, relatives with a history of affective disorders had a significantly higher ratio of mean red cell lithium to plasma lithium in vitro than relatives with no such history. A genetically controlled abnormality in lithium-sodium transport, the mechanism that determines the lithium ratio, may play a role in the etiology of some forms of affective disorders."}
{"id": "PMID:472717", "title": "Bitter taste of saccharin related to the genetic ability to taste the bitter substance 6-n-propylthiouracil.", "content": "Bitter taste thresholds for 6-n-propylthiouracil are bimodally distributed, dividing subjects into tasters and nontasters. Their taste worlds differ with regard to the sweetness of sucrose and saccharin and to the bitterness of saccharin. These differences suggest that nontasters tend to perceive less bitterness in saccharin at concentrations used in beverages.", "contents": "Bitter taste of saccharin related to the genetic ability to taste the bitter substance 6-n-propylthiouracil. Bitter taste thresholds for 6-n-propylthiouracil are bimodally distributed, dividing subjects into tasters and nontasters. Their taste worlds differ with regard to the sweetness of sucrose and saccharin and to the bitterness of saccharin. These differences suggest that nontasters tend to perceive less bitterness in saccharin at concentrations used in beverages."}
{"id": "PMID:472718", "title": "Insulin receptor binding in obesity: a reassessment.", "content": "A defect in the binding of insulin to circulating monocytes occurs when obese patients are hospitalized and fed a liberal carbohydrate diet. Under ordinary circumstances, most obese patients have normal insulin binding despite very high concentrations of serum insulin. These results show that insulin does not necessarily regulate its own receptor in vivo--as it does in vitro.", "contents": "Insulin receptor binding in obesity: a reassessment. A defect in the binding of insulin to circulating monocytes occurs when obese patients are hospitalized and fed a liberal carbohydrate diet. Under ordinary circumstances, most obese patients have normal insulin binding despite very high concentrations of serum insulin. These results show that insulin does not necessarily regulate its own receptor in vivo--as it does in vitro."}
{"id": "PMID:472719", "title": "Novel activation mechanism for the promutagenic herbicide diallate.", "content": "The potent bacterial mutagen 2-chloroacrolein is formed from the carcinogenic herbicide S-2,3-dichloroallyl diisopropylthiocarbamate (diallate) on incubation with hepatic microsomal monooxygenases or on reaction with m-chloroperbenzoic acid. A proposed activation mechanism for this promutagen involves sulfoxidation followed by [2,3] sigmatropic rearrangement and 1,2-elimination reactions. A portion of the highly reactive intermediate, diallate sulfoxide (proximate mutagens), is attacked by glutathione in a reaction which competes with its transformation to the ultimate mutagen, 2-chloroacrolein.", "contents": "Novel activation mechanism for the promutagenic herbicide diallate. The potent bacterial mutagen 2-chloroacrolein is formed from the carcinogenic herbicide S-2,3-dichloroallyl diisopropylthiocarbamate (diallate) on incubation with hepatic microsomal monooxygenases or on reaction with m-chloroperbenzoic acid. A proposed activation mechanism for this promutagen involves sulfoxidation followed by [2,3] sigmatropic rearrangement and 1,2-elimination reactions. A portion of the highly reactive intermediate, diallate sulfoxide (proximate mutagens), is attacked by glutathione in a reaction which competes with its transformation to the ultimate mutagen, 2-chloroacrolein."}
{"id": "PMID:472720", "title": "Visual resolution and receptive field size: examination of two kinds of cat retinal ganglion cell.", "content": "Intraocular recordings from brisk-sustained and brisk-transient ganglion cells in the cat's retina revealed a systematic increase in center size and decrease in spatial cut-off frequency with increasing distance from the area centralis. At any one eccentricity sizes of the centers of sustained and transient cells did not overlap, and the variation in cut-off frequency for each class was constrained to about one-half octave.", "contents": "Visual resolution and receptive field size: examination of two kinds of cat retinal ganglion cell. Intraocular recordings from brisk-sustained and brisk-transient ganglion cells in the cat's retina revealed a systematic increase in center size and decrease in spatial cut-off frequency with increasing distance from the area centralis. At any one eccentricity sizes of the centers of sustained and transient cells did not overlap, and the variation in cut-off frequency for each class was constrained to about one-half octave."}
{"id": "PMID:472721", "title": "The central origin of efferent pathways in the carotid sinus nerve of the cat.", "content": "The application of horseradish peroxidase to the central cut end of the carotid sinus nerve of the cat produced retrograde labeling of neurons in the ipsilateral medulla in the region of the nucleus ambiguus at anterior-posterior coordinates -8 to -10.5. These data coupled with previous electrophysiological observations suggest that the nucleus ambiguus may be the origin of an efferent inhibitory pathway to the carotid body.", "contents": "The central origin of efferent pathways in the carotid sinus nerve of the cat. The application of horseradish peroxidase to the central cut end of the carotid sinus nerve of the cat produced retrograde labeling of neurons in the ipsilateral medulla in the region of the nucleus ambiguus at anterior-posterior coordinates -8 to -10.5. These data coupled with previous electrophysiological observations suggest that the nucleus ambiguus may be the origin of an efferent inhibitory pathway to the carotid body."}
{"id": "PMID:472722", "title": "Saccharin metabolism and tumorigenicity.", "content": "Exposure of male Charles River CDI rats to a 5% saccharin diet in utero and throughout weaning, conditions associated with tumor induction, did not induce detectable metabolism (less than 0.4% of the oral dose) of tritiated saccharin in vivo. No metabolites (less than 0.06 microgram per kilogram per 24 hours) were detected in the urine of normal rats given a tracer dose. Pretreatment with 3-methylcholanthrene did not induce saccharin metabolism.", "contents": "Saccharin metabolism and tumorigenicity. Exposure of male Charles River CDI rats to a 5% saccharin diet in utero and throughout weaning, conditions associated with tumor induction, did not induce detectable metabolism (less than 0.4% of the oral dose) of tritiated saccharin in vivo. No metabolites (less than 0.06 microgram per kilogram per 24 hours) were detected in the urine of normal rats given a tracer dose. Pretreatment with 3-methylcholanthrene did not induce saccharin metabolism."}
{"id": "PMID:472723", "title": "Subfornical organ efferents to neural systems for control of body water.", "content": "The subfornical organ, a circumventricular structure of the central nervous system, has efferent neural projections to sites within the brain known to be involved in drinking behavior and secretion of antidiuretic hormone. By using anterograde tracing techniques, it is shown that the subfornical organ projects to the nucleus medians of the medial preoptic area, to the organum vasculosum of the lamina terminalis, and to the supraoptic nuclei bilaterally. Its efferent connectivity is confirmed by retrograde transport of horseradish peroxidase. The organum vasculosum of the lamina terminalis, another circumventricular organ and a suspected receptor site for angiotensin II, is involved in the circuitry of the subfornical organ and also has an efferent projection to the supraoptic nuclei.", "contents": "Subfornical organ efferents to neural systems for control of body water. The subfornical organ, a circumventricular structure of the central nervous system, has efferent neural projections to sites within the brain known to be involved in drinking behavior and secretion of antidiuretic hormone. By using anterograde tracing techniques, it is shown that the subfornical organ projects to the nucleus medians of the medial preoptic area, to the organum vasculosum of the lamina terminalis, and to the supraoptic nuclei bilaterally. Its efferent connectivity is confirmed by retrograde transport of horseradish peroxidase. The organum vasculosum of the lamina terminalis, another circumventricular organ and a suspected receptor site for angiotensin II, is involved in the circuitry of the subfornical organ and also has an efferent projection to the supraoptic nuclei."}
{"id": "PMID:472724", "title": "3-Methoxy-4-hydroxyphenethyleneglycol production by human brain in vivo.", "content": "A direct method has been employed to estimate the rate of production by human brain of 3-methoxy-4-hydroxyphenethyleneglycol, the major metabolite of brain norepinephrine, a brain neurotransmitter. Venous specimens were obtained from the internal jugular vein from ten awake human subjects at a puncture site above the common facial vein, the first major source of extracranial inflow. Arterial specimens were simultaneously obtained from the radial artery. Plasma samples were assayed and a highly significant difference was found in the concentration of the metabolite in plasma coming out of the brain (venous blood) as compared to plasma entering the brain (arterial blood). This venous-arterial difference was calculated to be 0.7 +/- 0.1 nanogram per milliliter of blood. Assuming an adult brain weight of 1400 grams and normal cerebral blood flow, it is estimated that the rate of production of 3-methoxy-4-hydroxyphenethyleneglycol by the awake human brain is approximately 597 nanograms per minute or 35.8 micrograms per hour. Urine specimens were also collected from six of these subjects during a period of 1 to 3.5 hours, which bracketed the time the blood samples were obtained. For these six subjects the output of 3-methyoxy-4-hydroxyphenethyleneglycol by whole brain was estimated to be 40.9 micrograms per hour, whereas the rate of its excretion into urine was 64.5 micrograms per hour.", "contents": "3-Methoxy-4-hydroxyphenethyleneglycol production by human brain in vivo. A direct method has been employed to estimate the rate of production by human brain of 3-methoxy-4-hydroxyphenethyleneglycol, the major metabolite of brain norepinephrine, a brain neurotransmitter. Venous specimens were obtained from the internal jugular vein from ten awake human subjects at a puncture site above the common facial vein, the first major source of extracranial inflow. Arterial specimens were simultaneously obtained from the radial artery. Plasma samples were assayed and a highly significant difference was found in the concentration of the metabolite in plasma coming out of the brain (venous blood) as compared to plasma entering the brain (arterial blood). This venous-arterial difference was calculated to be 0.7 +/- 0.1 nanogram per milliliter of blood. Assuming an adult brain weight of 1400 grams and normal cerebral blood flow, it is estimated that the rate of production of 3-methoxy-4-hydroxyphenethyleneglycol by the awake human brain is approximately 597 nanograms per minute or 35.8 micrograms per hour. Urine specimens were also collected from six of these subjects during a period of 1 to 3.5 hours, which bracketed the time the blood samples were obtained. For these six subjects the output of 3-methyoxy-4-hydroxyphenethyleneglycol by whole brain was estimated to be 40.9 micrograms per hour, whereas the rate of its excretion into urine was 64.5 micrograms per hour."}
{"id": "PMID:472725", "title": "Pigeons have magnets.", "content": "Research on pigeon homing suggests that magnetic field information is used for orientation. The ability of pigeons to sense magnetic fields may be associated with a small, unilateral structure between the brain and the skull which contains magnetic in what appears to be single domains.", "contents": "Pigeons have magnets. Research on pigeon homing suggests that magnetic field information is used for orientation. The ability of pigeons to sense magnetic fields may be associated with a small, unilateral structure between the brain and the skull which contains magnetic in what appears to be single domains."}
{"id": "PMID:472732", "title": "Enhanced 5-fluorouracil nucleotide formation after methotrexate administration: explanation for drug synergism.", "content": "Exposure of L1210 leukemia cells first to 0.1 to 100 micromolar methotrexate and then to 10 micromolar 5-fluorouracil produces a synergistic effect on the number of cells killed in culture. Methotrexate dose-related increases occur in the concentrations of intracellular 5-fluorouracil ribonucleotides and 5-fluoro-2'-deoxyuridylate and in the incorporation of 5-fluorouracil into RNA. These increases are correlated with increased concentrations of intracellular phosphoribosylpyrophosphate. It is proposed that the enhanced formation of ribonucleotides of 5-fluorouracil and the subsequent incorporation of these compounds into RNA in methotrexate-treated cells may account for synergism between these agents.", "contents": "Enhanced 5-fluorouracil nucleotide formation after methotrexate administration: explanation for drug synergism. Exposure of L1210 leukemia cells first to 0.1 to 100 micromolar methotrexate and then to 10 micromolar 5-fluorouracil produces a synergistic effect on the number of cells killed in culture. Methotrexate dose-related increases occur in the concentrations of intracellular 5-fluorouracil ribonucleotides and 5-fluoro-2'-deoxyuridylate and in the incorporation of 5-fluorouracil into RNA. These increases are correlated with increased concentrations of intracellular phosphoribosylpyrophosphate. It is proposed that the enhanced formation of ribonucleotides of 5-fluorouracil and the subsequent incorporation of these compounds into RNA in methotrexate-treated cells may account for synergism between these agents."}
{"id": "PMID:472733", "title": "Iodine-125--labeled estradiol: a gamma-emitting analog of estradiol that binds to the estrogen receptor.", "content": "17 beta-[16 alpha-125I]Iodoestradiol has been synthesized by exchange of 16 beta-bromoestradiol with Na125I. The iodinated product is readily separated from the bromo reactant by column chromatography. It concentrates in the rat uterus in vivo and binds avidly and specifically to the uterine estrogen receptor in vitro.", "contents": "Iodine-125--labeled estradiol: a gamma-emitting analog of estradiol that binds to the estrogen receptor. 17 beta-[16 alpha-125I]Iodoestradiol has been synthesized by exchange of 16 beta-bromoestradiol with Na125I. The iodinated product is readily separated from the bromo reactant by column chromatography. It concentrates in the rat uterus in vivo and binds avidly and specifically to the uterine estrogen receptor in vitro."}
{"id": "PMID:472734", "title": "Regrowth of severed axons in the neonatal central nervous system: establishment of normal connections.", "content": "When pyramidal tract axons are cut in the adult hamster, fibers degenerate in both anterograde and retrograde directions from the lesion. If the same operation is performed on infant hamsters, however, there is massive regrowth of the severed axons via a new brainstem pathway to their appropriate terminal sites in the medulla and spinal cord. In contrast to previous studies, these results suggest that axons in the mammalian central nervous system damaged early in life may regenerate in a functionally useful way.", "contents": "Regrowth of severed axons in the neonatal central nervous system: establishment of normal connections. When pyramidal tract axons are cut in the adult hamster, fibers degenerate in both anterograde and retrograde directions from the lesion. If the same operation is performed on infant hamsters, however, there is massive regrowth of the severed axons via a new brainstem pathway to their appropriate terminal sites in the medulla and spinal cord. In contrast to previous studies, these results suggest that axons in the mammalian central nervous system damaged early in life may regenerate in a functionally useful way."}
{"id": "PMID:472735", "title": "Plasma beta-endorphin immunoreactivity in schizophrenia.", "content": "Plasma beta-endorphin-like immunoreactivity was measured by a method that was equally sensitive to beta-endorphin and [Leu5]-beta-endorphin. Immunoreactivity in 98 schizophrenic patients did not differ greatly from that in 42 normal subjects. No immunoreactivity was detectable in dialyzates from first-time hemodialysis of eight nonpsychotic renal patients and nine schizophrenic patients. These results are not compatible with recent reports of extremely high concentrations of [Leu5]-beta-endorphin in hemodialyzates from schizophrenic patients.", "contents": "Plasma beta-endorphin immunoreactivity in schizophrenia. Plasma beta-endorphin-like immunoreactivity was measured by a method that was equally sensitive to beta-endorphin and [Leu5]-beta-endorphin. Immunoreactivity in 98 schizophrenic patients did not differ greatly from that in 42 normal subjects. No immunoreactivity was detectable in dialyzates from first-time hemodialysis of eight nonpsychotic renal patients and nine schizophrenic patients. These results are not compatible with recent reports of extremely high concentrations of [Leu5]-beta-endorphin in hemodialyzates from schizophrenic patients."}
{"id": "PMID:472738", "title": "Psychotropic drugs as behavioral teratogens.", "content": "Three psychotropic drugs were administered to pregnant rats and were then evaluated for their behavioral and reproductive effects in the offspring. Control rats received either saline or vitamin A. Prochlorperazine had the most disruptive effects on reproduction and growth, but had the least effect on behavior. Propoxyphene had no apparent effects on reproduction or growth, but produced a variety of behavioral changes. Fenfluramine was intermediate in its effects on reproduction and growth and had behavioral effects that were revealed in tests of preweaning development. The data suggest that systematic tests of behavior add important information to evaluations of reproductive toxicity that cannot, at present, be obtained by other means.", "contents": "Psychotropic drugs as behavioral teratogens. Three psychotropic drugs were administered to pregnant rats and were then evaluated for their behavioral and reproductive effects in the offspring. Control rats received either saline or vitamin A. Prochlorperazine had the most disruptive effects on reproduction and growth, but had the least effect on behavior. Propoxyphene had no apparent effects on reproduction or growth, but produced a variety of behavioral changes. Fenfluramine was intermediate in its effects on reproduction and growth and had behavioral effects that were revealed in tests of preweaning development. The data suggest that systematic tests of behavior add important information to evaluations of reproductive toxicity that cannot, at present, be obtained by other means."}
{"id": "PMID:472739", "title": "Geographic constraints on women's careers in academia.", "content": "Male and female academics have very different residence patterns. Women are more concentrated in our largest urban centers; also, wherever they reside, women are less likely than men to change communities when changing institutions. Much of this sex difference is attributable to the constraints under which married academic women must manage their careers, in particular the requirements of two-career households. The authors argue that the status difference between men and women in academia is attributable, in part, to the geographic limitations on the locations of married women, as these prevent making strategic job changes to advance career prospects.", "contents": "Geographic constraints on women's careers in academia. Male and female academics have very different residence patterns. Women are more concentrated in our largest urban centers; also, wherever they reside, women are less likely than men to change communities when changing institutions. Much of this sex difference is attributable to the constraints under which married academic women must manage their careers, in particular the requirements of two-career households. The authors argue that the status difference between men and women in academia is attributable, in part, to the geographic limitations on the locations of married women, as these prevent making strategic job changes to advance career prospects."}
{"id": "PMID:472741", "title": "N-nitrosamines in the rubber and tire industry.", "content": "Airborne N-nitrosomorpholine (0 to 27 micrograms per cubic meter) was found in two of four rubber industry factories. N-Nitrosodimethylamine was also found in two factories, but at lower levels. These findings may be relevant to the reported increased risk of certain types of cancer in rubber workers in some of the same areas where the N-nitrosomorpholine levels were highest.", "contents": "N-nitrosamines in the rubber and tire industry. Airborne N-nitrosomorpholine (0 to 27 micrograms per cubic meter) was found in two of four rubber industry factories. N-Nitrosodimethylamine was also found in two factories, but at lower levels. These findings may be relevant to the reported increased risk of certain types of cancer in rubber workers in some of the same areas where the N-nitrosomorpholine levels were highest."}
{"id": "PMID:472742", "title": "Sister chromatid exchanges in human lymphocytes after exposure to diagnostic ultrasound.", "content": "The frequency of sister chromatid exchanges increased in freshly isolated human lymphocytes as well as in a continuously growing lymphoblast line by exposure to diagnostic levels of ultrasound for 30 minutes. The results confirm previous findings indicating that ultrasound of diagnostic intensities can affect the DNA of animal cells.", "contents": "Sister chromatid exchanges in human lymphocytes after exposure to diagnostic ultrasound. The frequency of sister chromatid exchanges increased in freshly isolated human lymphocytes as well as in a continuously growing lymphoblast line by exposure to diagnostic levels of ultrasound for 30 minutes. The results confirm previous findings indicating that ultrasound of diagnostic intensities can affect the DNA of animal cells."}
{"id": "PMID:472743", "title": "Acridine araphanes: a new class of probe molecules for biological systems.", "content": "The bis-acridine ring system forms the basis for new biophysical probes of novel stereochemistry. Spectral data indicate that certain alkylene bridged bis-9-aminoacridines have a parallel plane conformation of predictable interplane distance. The parallel plane conformation is independent of solvent and thus is different from nucleic acid systems. This stable conformation allows these compounds to be used as sensitive \"rulers\" for describing binding site geometry in cholinergic enzymes and in the delineation of the mechanism of allosteric control in acetylcholinesterase.", "contents": "Acridine araphanes: a new class of probe molecules for biological systems. The bis-acridine ring system forms the basis for new biophysical probes of novel stereochemistry. Spectral data indicate that certain alkylene bridged bis-9-aminoacridines have a parallel plane conformation of predictable interplane distance. The parallel plane conformation is independent of solvent and thus is different from nucleic acid systems. This stable conformation allows these compounds to be used as sensitive \"rulers\" for describing binding site geometry in cholinergic enzymes and in the delineation of the mechanism of allosteric control in acetylcholinesterase."}
{"id": "PMID:472744", "title": "DNA synthesis and mitosis in adult amphibian cardiac muscle cells in vitro.", "content": "High-resolution autoradiography and fine structural analysis of adult newt heart tissue in long-term culture revealed that tritiated thymidine was concentrated in the nuclei of dedifferentiated myocardial cells. Mitotic chromosomes were observed in some of these cells. This demonstrates that adult amphibian myocardial cells in vitro are capable of DNA synthesis and mitosis.", "contents": "DNA synthesis and mitosis in adult amphibian cardiac muscle cells in vitro. High-resolution autoradiography and fine structural analysis of adult newt heart tissue in long-term culture revealed that tritiated thymidine was concentrated in the nuclei of dedifferentiated myocardial cells. Mitotic chromosomes were observed in some of these cells. This demonstrates that adult amphibian myocardial cells in vitro are capable of DNA synthesis and mitosis."}
{"id": "PMID:472745", "title": "Cerebral specialization, writing posture, and motor control of writing in left-handers.", "content": "It has been suggested that the inverted hand position of left-handers during writing indicates the left hemisphere of their brain is linguistically specialized and that the writing of these left-handers may be controlled via ipsilateral pathways. Electroencephalograph alpha asymmetry measures at central and parietal leads, as well as dichotic tests, differentiated right-handers from left-handers, but not inverters from noninverters. Electroencephalograph differences between hand posture groups did appear, but only at occipital leads during reading and writing tasks. Regardless of hand posture or speech lateralization, the right central region of the brain is significantly involved in the control of left-handed writing.", "contents": "Cerebral specialization, writing posture, and motor control of writing in left-handers. It has been suggested that the inverted hand position of left-handers during writing indicates the left hemisphere of their brain is linguistically specialized and that the writing of these left-handers may be controlled via ipsilateral pathways. Electroencephalograph alpha asymmetry measures at central and parietal leads, as well as dichotic tests, differentiated right-handers from left-handers, but not inverters from noninverters. Electroencephalograph differences between hand posture groups did appear, but only at occipital leads during reading and writing tasks. Regardless of hand posture or speech lateralization, the right central region of the brain is significantly involved in the control of left-handed writing."}
{"id": "PMID:472746", "title": "Pupillary responses during information processing vary with Scholastic Aptitude Test scores.", "content": "The magnitude of task-evoked pupillary dilations during mental activity has previously been shown to index the cognitive capacity utilized in the performance of the mental task. To determine the relation between \"intelligence\" and capacity demands during mental activity, task-evoked pupillary dilations were measured while two groups of university students differing in their scores on the Scholastic Aptitude Test solved mental arithmetic problems. Over three levels of problems difficulty, more intelligent subjects showed smaller task-evoked pupillary dilations than did their less intelligent counterparts. Thus, the more intelligent appear to possess more efficient cognitive structures of information processing. These data provide evidence that physiological differences between individuals of differing psychometric intelligence emerge during mental activity.", "contents": "Pupillary responses during information processing vary with Scholastic Aptitude Test scores. The magnitude of task-evoked pupillary dilations during mental activity has previously been shown to index the cognitive capacity utilized in the performance of the mental task. To determine the relation between \"intelligence\" and capacity demands during mental activity, task-evoked pupillary dilations were measured while two groups of university students differing in their scores on the Scholastic Aptitude Test solved mental arithmetic problems. Over three levels of problems difficulty, more intelligent subjects showed smaller task-evoked pupillary dilations than did their less intelligent counterparts. Thus, the more intelligent appear to possess more efficient cognitive structures of information processing. These data provide evidence that physiological differences between individuals of differing psychometric intelligence emerge during mental activity."}
{"id": "PMID:472747", "title": "Developmental fate of skeletal muscle satellite cells.", "content": "Radioisotopically labeled satellite cells from clonal cultures were implanted into normal muscle of the original donor. Implanted cells invariably retained their myogenic potential by participating in the regeneration of damaged myofibers or in the development of existing fibers.", "contents": "Developmental fate of skeletal muscle satellite cells. Radioisotopically labeled satellite cells from clonal cultures were implanted into normal muscle of the original donor. Implanted cells invariably retained their myogenic potential by participating in the regeneration of damaged myofibers or in the development of existing fibers."}
{"id": "PMID:472748", "title": "Auditory brainstem frequency following responses to waveform envelope periodicity.", "content": "An auditory frequency following response (FFR) was recorded to four complx stimuli. The FFR corresponded to the waveform envelope periodicity but not to the \"missing fundamental\" pitch of the stimuli. The FFR may be significant for timbre perception and sound lateralization.", "contents": "Auditory brainstem frequency following responses to waveform envelope periodicity. An auditory frequency following response (FFR) was recorded to four complx stimuli. The FFR corresponded to the waveform envelope periodicity but not to the \"missing fundamental\" pitch of the stimuli. The FFR may be significant for timbre perception and sound lateralization."}
{"id": "PMID:472749", "title": "Baroreceptor activation reduces reactivity to noxious stimulation: implications for hypertension.", "content": "The hypothesis was tested that an acute rise of blood pressure may reduce reactivity to noxious stimuli through a baroreceptor-mediated reduction of cerebral arousal. When blood pressure was raised by an infusion of phenylephrine, rats showed less running to terminate or avoid noxious stimuli than during saline infusions. This effect was not seen in rats with denervated baroreceptors. The results suggest that a rise of blood pressure could have motivational consequences significant for human hypertension.", "contents": "Baroreceptor activation reduces reactivity to noxious stimulation: implications for hypertension. The hypothesis was tested that an acute rise of blood pressure may reduce reactivity to noxious stimuli through a baroreceptor-mediated reduction of cerebral arousal. When blood pressure was raised by an infusion of phenylephrine, rats showed less running to terminate or avoid noxious stimuli than during saline infusions. This effect was not seen in rats with denervated baroreceptors. The results suggest that a rise of blood pressure could have motivational consequences significant for human hypertension."}
{"id": "PMID:472752", "title": "The hyperactive child syndrome.", "content": "The behavioral characteristics of the hyperactive child are described at different stages of development. Recent and sometimes controversial research findings are discussed with respect to prevalence, etiology, treatment, and outcome. It is concluded that this syndrome can best be understood in terms of interactions between social, psychological, and biological variables.", "contents": "The hyperactive child syndrome. The behavioral characteristics of the hyperactive child are described at different stages of development. Recent and sometimes controversial research findings are discussed with respect to prevalence, etiology, treatment, and outcome. It is concluded that this syndrome can best be understood in terms of interactions between social, psychological, and biological variables."}
{"id": "PMID:472754", "title": "Galactosemia: alterations in sulfate metabolism secondary to galactose-1-phosphate uridyltransferase deficiency.", "content": "Cultures of nonmutant as well as galactokinase-deficient fibroblasts incorporate 20 percent more [35S]sulfate when galactose is substituted for glucose in the medium; galactose-1-phosphate uridyltransferase-deficient cells incorporate 65.5 percent less. In addition to incorporating less [35S]sulfate, the uridyltransferase-deficient cells showed significant accumulation of intracellular galactose-1-phosphate within 4 hours after galactose exposure. Under the same conditions, no difference in [3H]uridine incorporation was observed. This metabolic alteration, occurring in response to galactose exposure, may be related to the pathophysiology of classical galactosemia.", "contents": "Galactosemia: alterations in sulfate metabolism secondary to galactose-1-phosphate uridyltransferase deficiency. Cultures of nonmutant as well as galactokinase-deficient fibroblasts incorporate 20 percent more [35S]sulfate when galactose is substituted for glucose in the medium; galactose-1-phosphate uridyltransferase-deficient cells incorporate 65.5 percent less. In addition to incorporating less [35S]sulfate, the uridyltransferase-deficient cells showed significant accumulation of intracellular galactose-1-phosphate within 4 hours after galactose exposure. Under the same conditions, no difference in [3H]uridine incorporation was observed. This metabolic alteration, occurring in response to galactose exposure, may be related to the pathophysiology of classical galactosemia."}
{"id": "PMID:472755", "title": "Meiotic maturation in Xenopus laevis oocytes initiated by insulin.", "content": "Insulin can induce meiotic division in Xenopus laevis oocytes. This effect shows the specificity expected of a receptor-mediated mechanism. It is potentiated by ethynylestradiol, a steroid antagonist of pregesterone (the natural hormone that provokes meiosis). The Xenopus laevis oocytes may serve as a model for the study of the poorly understood effect of insulin on cell division.", "contents": "Meiotic maturation in Xenopus laevis oocytes initiated by insulin. Insulin can induce meiotic division in Xenopus laevis oocytes. This effect shows the specificity expected of a receptor-mediated mechanism. It is potentiated by ethynylestradiol, a steroid antagonist of pregesterone (the natural hormone that provokes meiosis). The Xenopus laevis oocytes may serve as a model for the study of the poorly understood effect of insulin on cell division."}
{"id": "PMID:472756", "title": "Mutual repulsion between moving visual targets.", "content": "When two spatially intermingled sets of random dots move in different directions, the direction of each set may be misperceived. Observers report that each set of dots appears to move in a direction displaced by as much as 20 degrees from the direction of its companion set. Probably the result of inhibitory interactions, this mutual repulsion occurs at a central site in the visual system and may normally enhance discrimination of direction.", "contents": "Mutual repulsion between moving visual targets. When two spatially intermingled sets of random dots move in different directions, the direction of each set may be misperceived. Observers report that each set of dots appears to move in a direction displaced by as much as 20 degrees from the direction of its companion set. Probably the result of inhibitory interactions, this mutual repulsion occurs at a central site in the visual system and may normally enhance discrimination of direction."}
{"id": "PMID:472757", "title": "Human language cortex: localization of memory, syntax, and sequential motor-phoneme identification systems.", "content": "Subdivisions of the human peri-Sylvian language cortex were derived from stimulation mapping during craniotomies under local anesthesia. Naming, reading, short-term verbal memory, single and sequential orofacial movements, and phoneme identification were tested. Sequential orofacial movements and phoneme identification were altered from the same brain sites and thus identified a common system for language production and understanding. This system surrounded a final motor pathway for speech and was surrounded by a separate short-term verbal-memory system. Between the sequential motor-phoneme identification and memory systems were sites where only naming or reading were altered, including sites related exclusively by syntax.", "contents": "Human language cortex: localization of memory, syntax, and sequential motor-phoneme identification systems. Subdivisions of the human peri-Sylvian language cortex were derived from stimulation mapping during craniotomies under local anesthesia. Naming, reading, short-term verbal memory, single and sequential orofacial movements, and phoneme identification were tested. Sequential orofacial movements and phoneme identification were altered from the same brain sites and thus identified a common system for language production and understanding. This system surrounded a final motor pathway for speech and was surrounded by a separate short-term verbal-memory system. Between the sequential motor-phoneme identification and memory systems were sites where only naming or reading were altered, including sites related exclusively by syntax."}
{"id": "PMID:472758", "title": "Pseudohermaphroditism in Ilyanassa obsoleta (Mollusca: Neogastropoda).", "content": "Field studies, a rearing and maintenance program, and a transfer experiment have shown that anomalous male traits found in some female Ilyanassa obsoleta are an expression of environmentally controlled psuedohermaphroditism. The particular pattern of pseudohermaphroditism discovered in this species has not been reported previously from any other group, but may be characteristic of the Neogastropoda.", "contents": "Pseudohermaphroditism in Ilyanassa obsoleta (Mollusca: Neogastropoda). Field studies, a rearing and maintenance program, and a transfer experiment have shown that anomalous male traits found in some female Ilyanassa obsoleta are an expression of environmentally controlled psuedohermaphroditism. The particular pattern of pseudohermaphroditism discovered in this species has not been reported previously from any other group, but may be characteristic of the Neogastropoda."}
{"id": "PMID:472759", "title": "Receptor-mediated internalization of fluorescent chemotactic peptide by human neutrophils.", "content": "Tetramethylrhodamine labeled N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys is a potent chemoattractant for human neutrophils. Binding of this peptide to living neutrophils was observed by means of video intensification microscopy. At 37 degrees C, diffuse membrane fluorescence was seen initially, followed by rapid aggregation and internalization of the fluorescent peptide. These processes are dependent on specific binding to the formal peptide chemotactic receptor.", "contents": "Receptor-mediated internalization of fluorescent chemotactic peptide by human neutrophils. Tetramethylrhodamine labeled N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys is a potent chemoattractant for human neutrophils. Binding of this peptide to living neutrophils was observed by means of video intensification microscopy. At 37 degrees C, diffuse membrane fluorescence was seen initially, followed by rapid aggregation and internalization of the fluorescent peptide. These processes are dependent on specific binding to the formal peptide chemotactic receptor."}
{"id": "PMID:472760", "title": "Angiogenesis in the mouse cornea.", "content": "We have developed a method that permits analysis of neovascular responses in the mouse cornea. Using this method we have demonstrated that both allogeneic lymphocytes and a variety of tumors can induce angiogenesis, but that only the latter appear capable of eliciting secondary capillary sprouting.", "contents": "Angiogenesis in the mouse cornea. We have developed a method that permits analysis of neovascular responses in the mouse cornea. Using this method we have demonstrated that both allogeneic lymphocytes and a variety of tumors can induce angiogenesis, but that only the latter appear capable of eliciting secondary capillary sprouting."}
{"id": "PMID:472761", "title": "Imbalance in X-chromosome expression: evidence for a human X-linked gene affecting growth of hemopoietic cells.", "content": "In each of six family members who were heterozygous at the X-linked locus for glucose-6-phosphate dehydrogenase, only one or the other of the two alleles at that locus was almost exclusively expressed. The data are consistent with evidence that X-chromosome inactivation is a random process that may be followed by selection for one of the two resulting cell types on the basis of an unknown gene, which is located on the X chromosome and which can affect the rate of proliferation of hemopoietic cells in humans.", "contents": "Imbalance in X-chromosome expression: evidence for a human X-linked gene affecting growth of hemopoietic cells. In each of six family members who were heterozygous at the X-linked locus for glucose-6-phosphate dehydrogenase, only one or the other of the two alleles at that locus was almost exclusively expressed. The data are consistent with evidence that X-chromosome inactivation is a random process that may be followed by selection for one of the two resulting cell types on the basis of an unknown gene, which is located on the X chromosome and which can affect the rate of proliferation of hemopoietic cells in humans."}
{"id": "PMID:472762", "title": "Perinatal exposure to cannabinoids alters male reproductive function in mice.", "content": "Oral administration of delta 9-tetrahydrocannabinol or cannabinol to female mice late in pregnancy and during early lactation alters body weight regulation and pituitary-gonadal function and suppresses adult copulatory activity in their male offspring. These findings suggest that both psychoactive and nonpsychoactive constituents of marihuana can affect the development of male reproductive functions in mice.", "contents": "Perinatal exposure to cannabinoids alters male reproductive function in mice. Oral administration of delta 9-tetrahydrocannabinol or cannabinol to female mice late in pregnancy and during early lactation alters body weight regulation and pituitary-gonadal function and suppresses adult copulatory activity in their male offspring. These findings suggest that both psychoactive and nonpsychoactive constituents of marihuana can affect the development of male reproductive functions in mice."}
{"id": "PMID:472795", "title": "Systemic sarcoidosis presenting with a testicular nodule.", "content": "A patient presenting with the clinical and radiographic picture of pulmonary sarcoidosis was found to have a testicular nodule. Orchiectomy was done, and the pathologic specimen revealed only granulomatous inflammation. The involvement of the testis in sarcoidosis is reviewed, and recommendations are made regarding surgical diagnosis and medical treatment.", "contents": "Systemic sarcoidosis presenting with a testicular nodule. A patient presenting with the clinical and radiographic picture of pulmonary sarcoidosis was found to have a testicular nodule. Orchiectomy was done, and the pathologic specimen revealed only granulomatous inflammation. The involvement of the testis in sarcoidosis is reviewed, and recommendations are made regarding surgical diagnosis and medical treatment."}
{"id": "PMID:472796", "title": "Multifocal malignant melanoma of the esophagus.", "content": "We have described a case of primary melanoma of the esophagus in an asymptomatic patient. The tumor presented as a multinodular filling defect on a routine barium swallow. The distal esophagus and a proximal portion of the stomach were resected. The patient died ten days postoperatively of Klebsiella sepsis. Grossly, the surgical specimen showed multiple polypoid tumors which arose from separate pigmented areas shown microscopically to be melanoma in situ. By electron microscopy, the tumor cells contained numerous melanosomes in various stages of melanization. Primitive hemidesmosomes were found where a basal lamina was present. Better developed desmosomes interconnected the melanocytes.", "contents": "Multifocal malignant melanoma of the esophagus. We have described a case of primary melanoma of the esophagus in an asymptomatic patient. The tumor presented as a multinodular filling defect on a routine barium swallow. The distal esophagus and a proximal portion of the stomach were resected. The patient died ten days postoperatively of Klebsiella sepsis. Grossly, the surgical specimen showed multiple polypoid tumors which arose from separate pigmented areas shown microscopically to be melanoma in situ. By electron microscopy, the tumor cells contained numerous melanosomes in various stages of melanization. Primitive hemidesmosomes were found where a basal lamina was present. Better developed desmosomes interconnected the melanocytes."}
{"id": "PMID:472797", "title": "Giant cell tumor of the ischium: unusual site and outcome.", "content": "We have described an unusual case of giant cell tumor of the ischium. The remarkable features of the case are that it occurred in a relatively rare site and that it transformed radiologically from a localized, well circumscribed lesion into a very destructive lesion over a period of several months, though histologically it remained benign in appearance. The lesion was successfully treated by a modified hemipelvectomy. We emphasize that the treatment of choice in giant cell tumor of bone is total en bloc excision and not curettage or irradiation.", "contents": "Giant cell tumor of the ischium: unusual site and outcome. We have described an unusual case of giant cell tumor of the ischium. The remarkable features of the case are that it occurred in a relatively rare site and that it transformed radiologically from a localized, well circumscribed lesion into a very destructive lesion over a period of several months, though histologically it remained benign in appearance. The lesion was successfully treated by a modified hemipelvectomy. We emphasize that the treatment of choice in giant cell tumor of bone is total en bloc excision and not curettage or irradiation."}
{"id": "PMID:472798", "title": "Familial nonhemolytic jaundice with free and conjugated hyperbilirubinemia, elevated serum bile acids, and liver pigmentation.", "content": "We have described an 11-year-old patient with nonhemolytic hyperbilirubinemia, elevated serum bile acids, and liver pigmentation. The Bromsulphalein clearance was not typical of that seen in the Dubin-Johnson syndrome, but there was a secondary rise at 120 min. The abnormalities involving several organic anions and liver histology are compared to those seen in other types of familial nonhemolytic jaundice.", "contents": "Familial nonhemolytic jaundice with free and conjugated hyperbilirubinemia, elevated serum bile acids, and liver pigmentation. We have described an 11-year-old patient with nonhemolytic hyperbilirubinemia, elevated serum bile acids, and liver pigmentation. The Bromsulphalein clearance was not typical of that seen in the Dubin-Johnson syndrome, but there was a secondary rise at 120 min. The abnormalities involving several organic anions and liver histology are compared to those seen in other types of familial nonhemolytic jaundice."}
{"id": "PMID:472799", "title": "Severe pulmonary vascular disease in systemic lupus erythematosus.", "content": "A young woman with systemic lupus erythematosus (SLE) had clinical evidence of acute cor pulmonale. Autopsy disclosed vascular lesions in the lungs resembling those seen in advanced pulmonary hypertension. This case illustrates that severe pulmonary vascular disease may complicate SLE and mimic pulmonary thromboembolic disease.", "contents": "Severe pulmonary vascular disease in systemic lupus erythematosus. A young woman with systemic lupus erythematosus (SLE) had clinical evidence of acute cor pulmonale. Autopsy disclosed vascular lesions in the lungs resembling those seen in advanced pulmonary hypertension. This case illustrates that severe pulmonary vascular disease may complicate SLE and mimic pulmonary thromboembolic disease."}
{"id": "PMID:472800", "title": "Cantharidin: a valuable office treatment of molluscum contagiosum.", "content": "Cantharidin in either a 0.9% or 0.7% solution is a safe and effective office treatment for molluscum contagiosum. It simplifies the treatment, especially of children, by being a quick, simple, and painless procedure, but it should be applied only by the physician.", "contents": "Cantharidin: a valuable office treatment of molluscum contagiosum. Cantharidin in either a 0.9% or 0.7% solution is a safe and effective office treatment for molluscum contagiosum. It simplifies the treatment, especially of children, by being a quick, simple, and painless procedure, but it should be applied only by the physician."}
{"id": "PMID:472801", "title": "Periodontal disease: predisposing factor to chemotherapy-induced stomatitis.", "content": "Periodontal disease and dental plaque may predispose patients receiving antineoplastic drugs to stomatitis. Dental evaluation and appropriate treatment including oral hygiene are proposed to reduce the incidence and severity of stomatitis.", "contents": "Periodontal disease: predisposing factor to chemotherapy-induced stomatitis. Periodontal disease and dental plaque may predispose patients receiving antineoplastic drugs to stomatitis. Dental evaluation and appropriate treatment including oral hygiene are proposed to reduce the incidence and severity of stomatitis."}
{"id": "PMID:472804", "title": "Disulfiram-induced acute organic brain syndrome.", "content": "Reversible acute organic brain syndrome is described in a patient receiving disulfiram, 250 mg daily. Slowing of the electroencephalogram (3 to 4 cycles per second) in the occipital region resolved ten days after discontinuation of disulfiram. Acute organic brain syndrome induced by disulfiram is not rare but is often not correlated, and it should always be considered a possibility in patients receiving disulfiram therapy.", "contents": "Disulfiram-induced acute organic brain syndrome. Reversible acute organic brain syndrome is described in a patient receiving disulfiram, 250 mg daily. Slowing of the electroencephalogram (3 to 4 cycles per second) in the occipital region resolved ten days after discontinuation of disulfiram. Acute organic brain syndrome induced by disulfiram is not rare but is often not correlated, and it should always be considered a possibility in patients receiving disulfiram therapy."}
{"id": "PMID:472805", "title": "Life-threatening metabolic alkalosis in a comatose patient.", "content": "A 63-year-old man with obstructive pulmonary disease developed severe metablic alkalosis and coma while receiving steroid therapy and nasogastric suction. Treatment, which included the acute induction of hypercarbia and the simultaneous administration of acetazolamide and saline, restored acid-base balance within 24 hours. This combined approach eliminated the need to infuse hydrochloric acid.", "contents": "Life-threatening metabolic alkalosis in a comatose patient. A 63-year-old man with obstructive pulmonary disease developed severe metablic alkalosis and coma while receiving steroid therapy and nasogastric suction. Treatment, which included the acute induction of hypercarbia and the simultaneous administration of acetazolamide and saline, restored acid-base balance within 24 hours. This combined approach eliminated the need to infuse hydrochloric acid."}
{"id": "PMID:472806", "title": "Omental abscess: an unusual complication of cesarean section.", "content": "A case of an omental abscess following low transverse cesarean section is described. The incidence of cesarean section is rising, and with this rise one might expect an increase in the number and types of complications. A review of the literature fails to demonstrate any mention of this particular complication. In the evaluation of patients, one must consider not only the known but also the possible.", "contents": "Omental abscess: an unusual complication of cesarean section. A case of an omental abscess following low transverse cesarean section is described. The incidence of cesarean section is rising, and with this rise one might expect an increase in the number and types of complications. A review of the literature fails to demonstrate any mention of this particular complication. In the evaluation of patients, one must consider not only the known but also the possible."}
{"id": "PMID:472807", "title": "Cerebrospinal fluid rhinorrhea complicating pregnancy.", "content": "Cerebrospinal fluid rhinorrhea is an uncommone occurrence, especially when a history of previous trauma is absent. This report describes a case of cerebrospinal fluid rhinorrhea occurring in a pregnant patient during the pushing portion of her Lamaze exercises, and discusses the management of labor and delivery.", "contents": "Cerebrospinal fluid rhinorrhea complicating pregnancy. Cerebrospinal fluid rhinorrhea is an uncommone occurrence, especially when a history of previous trauma is absent. This report describes a case of cerebrospinal fluid rhinorrhea occurring in a pregnant patient during the pushing portion of her Lamaze exercises, and discusses the management of labor and delivery."}
{"id": "PMID:472810", "title": "Controversies and hazards in the treatment of pit viper bites.", "content": "This paper discusses the pathology produced by pit viper venom and the pharmacologically active substances released by the action of venom on human tissue. The risks of many of the popular methods of treatment such as tourniquets, cryotherapy, incisions, excisions, and cortisone are also discussed. A prospective treatment plan was devised which was limited to measures of proven benefit, and 28 patients were treated with no mortality or tissue necrosis.", "contents": "Controversies and hazards in the treatment of pit viper bites. This paper discusses the pathology produced by pit viper venom and the pharmacologically active substances released by the action of venom on human tissue. The risks of many of the popular methods of treatment such as tourniquets, cryotherapy, incisions, excisions, and cortisone are also discussed. A prospective treatment plan was devised which was limited to measures of proven benefit, and 28 patients were treated with no mortality or tissue necrosis."}
{"id": "PMID:472812", "title": "Surgical face lift.", "content": "The growing importance of the face lift and related procedures to improve the aging face and the increasing demand by both men and women for these procedures is largely due to the prolonged good health, active life, and extended working years of many people. The technic described emphasizes support of the facial tissues upward and backward, leaving the resulting scars in the least obvious locations. Illustrative cases show improvement in appearance by upper and lower blepharoplasty and by the temple, cheek, and neck lift. The importance of a \"segmental approach\" is mentioned and other accessory procedures which may contribute further to improvement of the aging face are described, including rhinoplasty, mentoplasty, surgical abrasion, and chemical peel.", "contents": "Surgical face lift. The growing importance of the face lift and related procedures to improve the aging face and the increasing demand by both men and women for these procedures is largely due to the prolonged good health, active life, and extended working years of many people. The technic described emphasizes support of the facial tissues upward and backward, leaving the resulting scars in the least obvious locations. Illustrative cases show improvement in appearance by upper and lower blepharoplasty and by the temple, cheek, and neck lift. The importance of a \"segmental approach\" is mentioned and other accessory procedures which may contribute further to improvement of the aging face are described, including rhinoplasty, mentoplasty, surgical abrasion, and chemical peel."}
{"id": "PMID:472813", "title": "Symptomatic lower esophageal ring: treatment of 24 patients.", "content": "The lower esophageal ring, or Schatzki's ring, consists of a thin, submucosal, circumferential scar which forms a thin incomplete diaphragm in the lower esophageal lumen. The symptoms may be either episodic aphagia or progressive dysphagia, and the severity of symptoms is related to the diameter of the ring. Between 1970 and 1978, we saw 24 patients with lower esophageal rings and complaints of episodic aphagia or progressive dysphagia. Symptoms of esophagitis were present in 20 of the 24. Twenty were treated surgically by interrupting the rings and repairing the sliding hiatal hernias. Two were treated by dilatation and two received no treatment to the ring. Hiatal hernias have recurred in two patients. In one, there is a recurrent ring and in the other, an acid peptic stricture. The ring has responded to dilatation and the peptic stricture to dilatation and repair of the recurrent hernia. Two patients without symptoms of esophagitis, treated by dilatation, are doing well but the follow-up period is so far too short to draw any conclusion.", "contents": "Symptomatic lower esophageal ring: treatment of 24 patients. The lower esophageal ring, or Schatzki's ring, consists of a thin, submucosal, circumferential scar which forms a thin incomplete diaphragm in the lower esophageal lumen. The symptoms may be either episodic aphagia or progressive dysphagia, and the severity of symptoms is related to the diameter of the ring. Between 1970 and 1978, we saw 24 patients with lower esophageal rings and complaints of episodic aphagia or progressive dysphagia. Symptoms of esophagitis were present in 20 of the 24. Twenty were treated surgically by interrupting the rings and repairing the sliding hiatal hernias. Two were treated by dilatation and two received no treatment to the ring. Hiatal hernias have recurred in two patients. In one, there is a recurrent ring and in the other, an acid peptic stricture. The ring has responded to dilatation and the peptic stricture to dilatation and repair of the recurrent hernia. Two patients without symptoms of esophagitis, treated by dilatation, are doing well but the follow-up period is so far too short to draw any conclusion."}
{"id": "PMID:472814", "title": "Hemiagenesis of the thyroid.", "content": "Congenital absence of a lobe of the thyroid gland is discussed and the literature is reviewed. Sixty-six cases have been gathered from the literature in approximately the past 100 years, and another six have been documented. Although no particular explanation has been forthcoming for the development of this anomaly, left lobe aplasia and predominance of occurrence in women have been noted. A variety of other thyroidal abnormalities have coexisted with hemiagenesis.", "contents": "Hemiagenesis of the thyroid. Congenital absence of a lobe of the thyroid gland is discussed and the literature is reviewed. Sixty-six cases have been gathered from the literature in approximately the past 100 years, and another six have been documented. Although no particular explanation has been forthcoming for the development of this anomaly, left lobe aplasia and predominance of occurrence in women have been noted. A variety of other thyroidal abnormalities have coexisted with hemiagenesis."}
{"id": "PMID:472815", "title": "Carcinomatous meningitis in gastrointestinal malignancies.", "content": "With the development of the Millipore filter, meningeal metastases are being identified more frequently as a complication of many types of carcinoma, including breast, lung, pancreas, stomach, and prostate. Regardless of the primary source, adenocarcinoma cells appear to have a propensity to metastasize to this site. In recent years, carcinomatous meningitis has been reported very infrequently in gastrointestinal malignancies, as compared with other primary sites. We present two cases of carcinomatous meningitis, one in a 29-year-old man with adenocarcinoma of the colon and another in a 51-year-old man with gastric carcinoma. Survival after this complication is presently poor, but treatment may offer amelioration of disabling symptoms. The aggressive nature of these metastases emphasizes the need for early clinical suspicion and examination of spinal fluid in patients with gastrointestinal malignancies.", "contents": "Carcinomatous meningitis in gastrointestinal malignancies. With the development of the Millipore filter, meningeal metastases are being identified more frequently as a complication of many types of carcinoma, including breast, lung, pancreas, stomach, and prostate. Regardless of the primary source, adenocarcinoma cells appear to have a propensity to metastasize to this site. In recent years, carcinomatous meningitis has been reported very infrequently in gastrointestinal malignancies, as compared with other primary sites. We present two cases of carcinomatous meningitis, one in a 29-year-old man with adenocarcinoma of the colon and another in a 51-year-old man with gastric carcinoma. Survival after this complication is presently poor, but treatment may offer amelioration of disabling symptoms. The aggressive nature of these metastases emphasizes the need for early clinical suspicion and examination of spinal fluid in patients with gastrointestinal malignancies."}
{"id": "PMID:472816", "title": "Cervical cerclage: twenty years' experience.", "content": "During a 20-year period, 40 pregnancies were managed in 32 patients with a diagnosis of an incompetent cervix, a ratio of 1:775 deliveries. Diagnosis before development of symptoms produced a 92% fetal salvage, with 68% reaching 36 weeks of gestation. Development of symptoms before cerclage resulted in only a 40% fetal salvage. Fetal salvage was reduced in cases where membranes bulged beyond the cervix, the cervix was more than 3 cm dilated, or the gestational age was greater than 26 weeks at the time of suture placement. No difference was noted between the Shirodkar and McDonald procedures. Major complications included uterine rupture in three patients and acute chorioamnionitis in two. Because of complications, treatment is contraindicated based on history alone or where close follow-up is not available.", "contents": "Cervical cerclage: twenty years' experience. During a 20-year period, 40 pregnancies were managed in 32 patients with a diagnosis of an incompetent cervix, a ratio of 1:775 deliveries. Diagnosis before development of symptoms produced a 92% fetal salvage, with 68% reaching 36 weeks of gestation. Development of symptoms before cerclage resulted in only a 40% fetal salvage. Fetal salvage was reduced in cases where membranes bulged beyond the cervix, the cervix was more than 3 cm dilated, or the gestational age was greater than 26 weeks at the time of suture placement. No difference was noted between the Shirodkar and McDonald procedures. Major complications included uterine rupture in three patients and acute chorioamnionitis in two. Because of complications, treatment is contraindicated based on history alone or where close follow-up is not available."}
{"id": "PMID:472818", "title": "Ligation of the superior vena cava for recurrent pulmonary emboli.", "content": "A patient with polycythemia vera developed chronic thrombosis of the superior vena cava and had life-threatening pulmonary emboli despite anticoagulation and chemotherapy for the blood dyscrasia. After angiographic evaluation, the superior vena cava was ligated cephalad to the azygous vein. There have been no further clinical embolic episodes nor adverse effects over a three-year period of observation.", "contents": "Ligation of the superior vena cava for recurrent pulmonary emboli. A patient with polycythemia vera developed chronic thrombosis of the superior vena cava and had life-threatening pulmonary emboli despite anticoagulation and chemotherapy for the blood dyscrasia. After angiographic evaluation, the superior vena cava was ligated cephalad to the azygous vein. There have been no further clinical embolic episodes nor adverse effects over a three-year period of observation."}
{"id": "PMID:472819", "title": "Pulmonary function tests in a community hospital.", "content": "The purpose of this study was to determine the incidence of obstructive lung disease in a surgical population. Screening spirometric pulmonary function tests were performed in 300 consecutive ambulatory patients in a community hospital. A high incidence of moderate to very severe chronic obstructive lung disease was found, 66% of smokers and 38% of nonsmokers. The incidence of chronic obstructive lung disease is underestimated. The costs of this disease are staggering in terms of money and manpower, and there is great need for screening pulmonary function testing to promote early diagnosis. The community hospital appears to be the ideal environment in which to make an early diagnosis and initiate measures to attempt to change the natural history of chronic obstructive lung disease.", "contents": "Pulmonary function tests in a community hospital. The purpose of this study was to determine the incidence of obstructive lung disease in a surgical population. Screening spirometric pulmonary function tests were performed in 300 consecutive ambulatory patients in a community hospital. A high incidence of moderate to very severe chronic obstructive lung disease was found, 66% of smokers and 38% of nonsmokers. The incidence of chronic obstructive lung disease is underestimated. The costs of this disease are staggering in terms of money and manpower, and there is great need for screening pulmonary function testing to promote early diagnosis. The community hospital appears to be the ideal environment in which to make an early diagnosis and initiate measures to attempt to change the natural history of chronic obstructive lung disease."}
{"id": "PMID:472820", "title": "Correlation of intraoperative electroencephalography with neurologic deficit after carotid endarterectomy.", "content": "In an attempt to prevent morbidity and mortality in carotid endarterectomy, we monitored 47 operations with intraoperative EEG to determine which patients should have a shunt during endarterectomy. The EEG was recorded for up to five minutes during test carotid cross-clamping. When EEG asymmetry between the cerebral hemispheres occurred, the clamp was immediately removed. A shunt was used in all patients who had EEG asymmetry. In the absence of EEG asymmetry, no shunt was used. Of the 38 patients with no EEG asymmetry intraoperatively, one patient had transient deficits postoperatively. Electroencephalographic asymmetry occurred in nine patients during test carotid cross-clamping. Postoperatively, five of these shunted patients awakened neurologically intact and four patients had transient deficit. When the EEG was normal and no shunt was used, patients did well; when the EEG was abnormal and a shunt was used, there was a high incidence of transient neurologic deficit.", "contents": "Correlation of intraoperative electroencephalography with neurologic deficit after carotid endarterectomy. In an attempt to prevent morbidity and mortality in carotid endarterectomy, we monitored 47 operations with intraoperative EEG to determine which patients should have a shunt during endarterectomy. The EEG was recorded for up to five minutes during test carotid cross-clamping. When EEG asymmetry between the cerebral hemispheres occurred, the clamp was immediately removed. A shunt was used in all patients who had EEG asymmetry. In the absence of EEG asymmetry, no shunt was used. Of the 38 patients with no EEG asymmetry intraoperatively, one patient had transient deficits postoperatively. Electroencephalographic asymmetry occurred in nine patients during test carotid cross-clamping. Postoperatively, five of these shunted patients awakened neurologically intact and four patients had transient deficit. When the EEG was normal and no shunt was used, patients did well; when the EEG was abnormal and a shunt was used, there was a high incidence of transient neurologic deficit."}
{"id": "PMID:472821", "title": "A new problem in adolescent gynecology.", "content": "As more abortions are being done in teen-age girls than ever before, an unusually large number of complications are being seen by some private practitioners. Because many of these adolescent patients in whom complications develop do not return to the physicians who did the abortion, accurate data on the incidence of abortion complications are difficult to obtain. It is only reasonable that the teen-ager be given all the facts available on the significant complications of abortion and its possible effects on future childbearing.", "contents": "A new problem in adolescent gynecology. As more abortions are being done in teen-age girls than ever before, an unusually large number of complications are being seen by some private practitioners. Because many of these adolescent patients in whom complications develop do not return to the physicians who did the abortion, accurate data on the incidence of abortion complications are difficult to obtain. It is only reasonable that the teen-ager be given all the facts available on the significant complications of abortion and its possible effects on future childbearing."}
{"id": "PMID:472822", "title": "Diagnostic exploratory celiotomy: an outdated concept in blunt abdominal trauma.", "content": "Diagnostic exploratory celiotomy as the primary procedure in the trauma patient is rarely indicated since the validation of peritoneal lavage as a diagnostic technic. The accuracy of diagnostic peritoneal lavage is clearly superior to that of initial or repetitive clinical evaluation of trauma patients, and its cost is miniscule compared with unnecessary diagnostic celiotomy.", "contents": "Diagnostic exploratory celiotomy: an outdated concept in blunt abdominal trauma. Diagnostic exploratory celiotomy as the primary procedure in the trauma patient is rarely indicated since the validation of peritoneal lavage as a diagnostic technic. The accuracy of diagnostic peritoneal lavage is clearly superior to that of initial or repetitive clinical evaluation of trauma patients, and its cost is miniscule compared with unnecessary diagnostic celiotomy."}
{"id": "PMID:472824", "title": "Umbilical anomalies.", "content": "During intrauterine development the primordia of the developing gastrointestinal and urinary tract come into close proximity in the umbilicus where the communication of these structures with the external environment is usually obliterated. In a small percentage of patients the omphalomesenteric duct and/or urachus may remain completely or partially patent. When complete patency is present, drainage of mucus, stool, or urine may occur. Partial patency may be manifest by an abdominal mass or by no symptoms at all. Definitive treatment includes total excision of the omphalomesenteric duct and/or urachus, lest recanalization of remnant tissue or carcinoma in later life occur.", "contents": "Umbilical anomalies. During intrauterine development the primordia of the developing gastrointestinal and urinary tract come into close proximity in the umbilicus where the communication of these structures with the external environment is usually obliterated. In a small percentage of patients the omphalomesenteric duct and/or urachus may remain completely or partially patent. When complete patency is present, drainage of mucus, stool, or urine may occur. Partial patency may be manifest by an abdominal mass or by no symptoms at all. Definitive treatment includes total excision of the omphalomesenteric duct and/or urachus, lest recanalization of remnant tissue or carcinoma in later life occur."}
{"id": "PMID:472825", "title": "Paramedical programs in rural Bangladesh.", "content": "Two programs using locally trained paramedical personnel provide health care to villagers in Bangladesh. The general level of care rendered appears to be above average for the country, but varies with the individual paramedical worker. Field supervision is crucial to quality of care. The structure of the two programs places physicians in direct contact with the paramedical workers for supervision and consultation. Unlike most programs in less developed countries, these emphasize preventive care, prepaid insurance at minimal premiums (in one plan, premiums are payable by barter), and apparently high motivation of the staff, many showing personal concern as well as evidence of sound training.", "contents": "Paramedical programs in rural Bangladesh. Two programs using locally trained paramedical personnel provide health care to villagers in Bangladesh. The general level of care rendered appears to be above average for the country, but varies with the individual paramedical worker. Field supervision is crucial to quality of care. The structure of the two programs places physicians in direct contact with the paramedical workers for supervision and consultation. Unlike most programs in less developed countries, these emphasize preventive care, prepaid insurance at minimal premiums (in one plan, premiums are payable by barter), and apparently high motivation of the staff, many showing personal concern as well as evidence of sound training."}
{"id": "PMID:472826", "title": "Echocardiographic swinging heart motion in loculated anterior pericardial effusion.", "content": "A patient in chronic renal failure presented with cardiomegaly. Echocardiography and cardiac scan demonstrated loculated anterior pericardial effusion. Another unusual echocardiographic feature was the presence of swinging heart motion, usually seen only with large nonlocalized pericardial effusion. When dialysis led to substantial regression of cardiac size, the echocardiographic abnormalities disappeared.", "contents": "Echocardiographic swinging heart motion in loculated anterior pericardial effusion. A patient in chronic renal failure presented with cardiomegaly. Echocardiography and cardiac scan demonstrated loculated anterior pericardial effusion. Another unusual echocardiographic feature was the presence of swinging heart motion, usually seen only with large nonlocalized pericardial effusion. When dialysis led to substantial regression of cardiac size, the echocardiographic abnormalities disappeared."}
{"id": "PMID:472829", "title": "Lung cancer in shipbuilding and related industries in Louisiana.", "content": "The relationship between shipbuilding and related industries and risk of fatal lung cancer (1960-1975) is described for selected Louisiana parishes. Deaths from lung cancer were matched to deaths not caused by cancer. Shipbuilders had a significantly increased risk (greater than twofold) of dying of lung cancer as compared with other causes. The risk of dying of lung cancer in related occupations (seamen and longshoremen) was also increased. Information on laterality of lung cancer was not supportive of particulate substances contributing to causality due to the large number of unspecified cases. The preponderance of deaths appears to be occurring in men with a greater number of years of exposure to this industry and in those aged 20 to 34 years in 1940. These common occupations in Louisiana could contribute to the high rate of lung cancer.", "contents": "Lung cancer in shipbuilding and related industries in Louisiana. The relationship between shipbuilding and related industries and risk of fatal lung cancer (1960-1975) is described for selected Louisiana parishes. Deaths from lung cancer were matched to deaths not caused by cancer. Shipbuilders had a significantly increased risk (greater than twofold) of dying of lung cancer as compared with other causes. The risk of dying of lung cancer in related occupations (seamen and longshoremen) was also increased. Information on laterality of lung cancer was not supportive of particulate substances contributing to causality due to the large number of unspecified cases. The preponderance of deaths appears to be occurring in men with a greater number of years of exposure to this industry and in those aged 20 to 34 years in 1940. These common occupations in Louisiana could contribute to the high rate of lung cancer."}
{"id": "PMID:472830", "title": "Anesthetic management of neck trauma.", "content": "In a retrospective review of one year's experience in the management of neck trauma, 88 cases comprising 42 gunshot wounds, 29 stab or slash injuries, and 17 injuries by blunt trauma were reviewed. Multiple injuries were noted in all groups, but a higher percentage was found in the blunt trauma series. Anesthetic management is reviewed, and emphasis is placed on careful evaluation of the patient's airway, using physical and roentgenographic examination to plan adequate airway management.", "contents": "Anesthetic management of neck trauma. In a retrospective review of one year's experience in the management of neck trauma, 88 cases comprising 42 gunshot wounds, 29 stab or slash injuries, and 17 injuries by blunt trauma were reviewed. Multiple injuries were noted in all groups, but a higher percentage was found in the blunt trauma series. Anesthetic management is reviewed, and emphasis is placed on careful evaluation of the patient's airway, using physical and roentgenographic examination to plan adequate airway management."}
{"id": "PMID:472831", "title": "Laryngeal papillomatosis: anesthetic management.", "content": "We reviewed the anesthetic management of all children admitted with laryngeal papillomatosis during a six-year period. This included 147 endoscopies on 16 patients, most referred with advanced disease after initial care in other hospitals. Onset of the disease before age 2 was associated with the most severe upper airway involvement. Eight patients required tracheostomy at some time during treatment, and management of the shared airway during surgical manipulation was particularly difficult in these children. Inhalational induction with halothane was used rountinely, with surprisingly few instances of laryngospasm or upper airway obstruction. Topical lidocaine spray and orotracheal intubation were preferred; insufflation technics were frequently abandoned when attempted. Extubation under deep anesthesia after clearing the glottis of secretions was usually followed by smooth emergence and minimal airway difficulties. There was only one instance of postoperative stridor suggesting croup. A standard anesthetic approach can be recommended, on the basis of the experience in this series, but possible variations in technic are also discussed for particular clinical applications.", "contents": "Laryngeal papillomatosis: anesthetic management. We reviewed the anesthetic management of all children admitted with laryngeal papillomatosis during a six-year period. This included 147 endoscopies on 16 patients, most referred with advanced disease after initial care in other hospitals. Onset of the disease before age 2 was associated with the most severe upper airway involvement. Eight patients required tracheostomy at some time during treatment, and management of the shared airway during surgical manipulation was particularly difficult in these children. Inhalational induction with halothane was used rountinely, with surprisingly few instances of laryngospasm or upper airway obstruction. Topical lidocaine spray and orotracheal intubation were preferred; insufflation technics were frequently abandoned when attempted. Extubation under deep anesthesia after clearing the glottis of secretions was usually followed by smooth emergence and minimal airway difficulties. There was only one instance of postoperative stridor suggesting croup. A standard anesthetic approach can be recommended, on the basis of the experience in this series, but possible variations in technic are also discussed for particular clinical applications."}
{"id": "PMID:472832", "title": "Adverse effects of pancuronium in patients with hemorrhagic shock.", "content": "Thirty-five patients with hemorrhagic shock had surgical repair of trauma to the great vessels using a ketamine-pancuronium-nitrous oxide sequence for anesthesia. The data of the cardiovascular and respiratory changes occurring during anesthesia and the operation were tabulated and subjected to multifactorial computer analysis. Fifteen patients died during or after operation because the injury was beyond repair. Twenty survived the oepration. Profound hypotension associated with mild tachycardia occurred with surprising regularity each time incremental doses of pancuronium were administered in nearly all patients. This hypotension was not associated with the administration of any other drug or with any other parameter and strongly implicates pancuronium as the causative factor. Pancuronium should be used cautiously and administered in small increments to this type of patient if it is used as a muscle relaxant.", "contents": "Adverse effects of pancuronium in patients with hemorrhagic shock. Thirty-five patients with hemorrhagic shock had surgical repair of trauma to the great vessels using a ketamine-pancuronium-nitrous oxide sequence for anesthesia. The data of the cardiovascular and respiratory changes occurring during anesthesia and the operation were tabulated and subjected to multifactorial computer analysis. Fifteen patients died during or after operation because the injury was beyond repair. Twenty survived the oepration. Profound hypotension associated with mild tachycardia occurred with surprising regularity each time incremental doses of pancuronium were administered in nearly all patients. This hypotension was not associated with the administration of any other drug or with any other parameter and strongly implicates pancuronium as the causative factor. Pancuronium should be used cautiously and administered in small increments to this type of patient if it is used as a muscle relaxant."}
{"id": "PMID:472833", "title": "Auriculotemporal syndrome after combined modality therapy for cancer.", "content": "Auriculotemporal syndrome frequently follows trauma to the auriculotemporal nerve, a branch of the mandibular nerve. Classic symptoms are gustatory sweating and hyperemia, with pain occurring in less than 10% of cases. We have treated six patients for a variant of auriculotemporal syndrome following combined modality therapy for head and neck cancers. Unique was the presence of pain and the absence of gustatory sweating in all cases. The combination of radical surgery, chemotherapy, and irradiation obscured the \"textbook\" presentation, leading to initial misdiagnosis. Long-term relief (one year) from this atypical presentation of auriculotemporal syndrome was afforded by a simple mandibular nerve block, a technic that is of no sustained therapeutic benefit in the classic cases.", "contents": "Auriculotemporal syndrome after combined modality therapy for cancer. Auriculotemporal syndrome frequently follows trauma to the auriculotemporal nerve, a branch of the mandibular nerve. Classic symptoms are gustatory sweating and hyperemia, with pain occurring in less than 10% of cases. We have treated six patients for a variant of auriculotemporal syndrome following combined modality therapy for head and neck cancers. Unique was the presence of pain and the absence of gustatory sweating in all cases. The combination of radical surgery, chemotherapy, and irradiation obscured the \"textbook\" presentation, leading to initial misdiagnosis. Long-term relief (one year) from this atypical presentation of auriculotemporal syndrome was afforded by a simple mandibular nerve block, a technic that is of no sustained therapeutic benefit in the classic cases."}
{"id": "PMID:472834", "title": "Sputum cytology in the diagnosis and treatment of lung cancer.", "content": "To evaluate the contribution of sputum cytology to the diagnosis and treatment of lung cancer, 918 cases of lung cancer were reviewed. Of 105 patients with positive sputum cytology, 93 had other invasive procedures to establish the cell type or stage or to attempt to cure the disease. In 12 patients the presence of positive sputum prevented further invasive evaluation. Thus, the real benefit of sputum cytology was reduced to 12 patients, or little over 1% of the total number of lung cancer patients. It is concluded that sputum cytology is not an effective diagnostic approach to suspected lung cancer. It rarely precluded invasive proceudres because it failed to contribute to the staging of the disease. Therefore, it should be used in only those patients in whom the yield of positive sputum would direct therapy other than surgery.", "contents": "Sputum cytology in the diagnosis and treatment of lung cancer. To evaluate the contribution of sputum cytology to the diagnosis and treatment of lung cancer, 918 cases of lung cancer were reviewed. Of 105 patients with positive sputum cytology, 93 had other invasive procedures to establish the cell type or stage or to attempt to cure the disease. In 12 patients the presence of positive sputum prevented further invasive evaluation. Thus, the real benefit of sputum cytology was reduced to 12 patients, or little over 1% of the total number of lung cancer patients. It is concluded that sputum cytology is not an effective diagnostic approach to suspected lung cancer. It rarely precluded invasive proceudres because it failed to contribute to the staging of the disease. Therefore, it should be used in only those patients in whom the yield of positive sputum would direct therapy other than surgery."}
{"id": "PMID:472835", "title": "Histoplasmosis: clinical manifestations and surgical management.", "content": "In this retrospective study of 115 cases of histoplasmids, there were 66 male and 49 female patients ranging in age from 2 months to 79 years. The most common presenting symptoms were cough, chest pain, wheezing, weight loss, hemoptysis, and shortness of breath. Thirty-five patients (30%) were asymptomatic. Two patients had manifestations of obstruction of the superior vena cava. Radiologic findings simulated carcinoma, tuberculosis, pneumonia, and viral infections. Sixty-five patients had various operative proceudres, such as lung biopsy, wedge resection, lobectomy, pneumonectomy, resection of lymph node, and bypass of superior vena cava, for diagnosis and treatment. There were two deaths and two postoperative complications. A total of 15 patients received intravenous amphotericin B. Four patients with pneumonic infiltrates developed disseminated histoplasmosis.", "contents": "Histoplasmosis: clinical manifestations and surgical management. In this retrospective study of 115 cases of histoplasmids, there were 66 male and 49 female patients ranging in age from 2 months to 79 years. The most common presenting symptoms were cough, chest pain, wheezing, weight loss, hemoptysis, and shortness of breath. Thirty-five patients (30%) were asymptomatic. Two patients had manifestations of obstruction of the superior vena cava. Radiologic findings simulated carcinoma, tuberculosis, pneumonia, and viral infections. Sixty-five patients had various operative proceudres, such as lung biopsy, wedge resection, lobectomy, pneumonectomy, resection of lymph node, and bypass of superior vena cava, for diagnosis and treatment. There were two deaths and two postoperative complications. A total of 15 patients received intravenous amphotericin B. Four patients with pneumonic infiltrates developed disseminated histoplasmosis."}
{"id": "PMID:472837", "title": "Cutaneous horns: a histopathologic study.", "content": "We report a series of 230 cutaneous horns, of which fewer than one fourth were frankly malignant. Actinic keratoses were the lesions most commonly found underlying cutaneous horns (37.39%). Cutaneous horns overlying a benign lichenoid keratosis, epidermolytic hyperkeratosis, trichilemmoma, an epidermal inclusion cyst, and a benign fibroma are reported. Three cases were nondiagnosable because of inadequate biopsies that were too superficial to show the base of the lesion.", "contents": "Cutaneous horns: a histopathologic study. We report a series of 230 cutaneous horns, of which fewer than one fourth were frankly malignant. Actinic keratoses were the lesions most commonly found underlying cutaneous horns (37.39%). Cutaneous horns overlying a benign lichenoid keratosis, epidermolytic hyperkeratosis, trichilemmoma, an epidermal inclusion cyst, and a benign fibroma are reported. Three cases were nondiagnosable because of inadequate biopsies that were too superficial to show the base of the lesion."}
{"id": "PMID:472838", "title": "Diagnosis and treatment of nonorganic pelvic pain.", "content": "Pelvic pain was a prominent complaint among one third of 3,000 gynecology clinic patients. In 1.1% no causative disease could be found, and these patients failed to respond to routine therapy. Twenty of these patients who were studied minutely revealed some psychologic disorder including hysteria, passive-aggressive behavior, sociopathy, depression, and alcoholism. A strong tendency toward psychophysiologic disturbances in other systems was practically universal. Supportive psychotherapy, progressive relaxation training, and insight therapy were used. The greatest barrier to treatment success was refusal of patients to accept the psychologic factors in their total illness.", "contents": "Diagnosis and treatment of nonorganic pelvic pain. Pelvic pain was a prominent complaint among one third of 3,000 gynecology clinic patients. In 1.1% no causative disease could be found, and these patients failed to respond to routine therapy. Twenty of these patients who were studied minutely revealed some psychologic disorder including hysteria, passive-aggressive behavior, sociopathy, depression, and alcoholism. A strong tendency toward psychophysiologic disturbances in other systems was practically universal. Supportive psychotherapy, progressive relaxation training, and insight therapy were used. The greatest barrier to treatment success was refusal of patients to accept the psychologic factors in their total illness."}
{"id": "PMID:472839", "title": "Lymphocyte toxicity in retinoblastoma.", "content": "Cytotoxicity can be used in the determination of patients with retinoblastoma. With further refinement, this type of test may become useful in aiding the physician in the detection of retinoblastoma.", "contents": "Lymphocyte toxicity in retinoblastoma. Cytotoxicity can be used in the determination of patients with retinoblastoma. With further refinement, this type of test may become useful in aiding the physician in the detection of retinoblastoma."}
{"id": "PMID:472840", "title": "Phylogeny and the herniated disc.", "content": "Using a phylogenic classification of the spine, one can identify precisely the numerical location of any vertebra or disc, including vertebrae with junctional anomalies at transitional zones. Such precision permitted comparisons of myelographic reports of sites of disc disease with the actual levels of extradural deformities and of charted levels of disc operations with the actual levels of disc curettements. Among 100 patients there were 33 with phylogenic or junctional departures from the human mode. Among these 33 there was a combined total of 18 identification errors, and there were four entries at incorrect levels. Preoperative landmarking would appear to be obligatory.", "contents": "Phylogeny and the herniated disc. Using a phylogenic classification of the spine, one can identify precisely the numerical location of any vertebra or disc, including vertebrae with junctional anomalies at transitional zones. Such precision permitted comparisons of myelographic reports of sites of disc disease with the actual levels of extradural deformities and of charted levels of disc operations with the actual levels of disc curettements. Among 100 patients there were 33 with phylogenic or junctional departures from the human mode. Among these 33 there was a combined total of 18 identification errors, and there were four entries at incorrect levels. Preoperative landmarking would appear to be obligatory."}
{"id": "PMID:472841", "title": "Screening for colorectal cancer.", "content": "A voluntary colorectal screening program was carried out in North Carolina to evaluate the public's willingness to perform a do-it-yourself bowel test, to educate the public on the importance of routine colorectal screening, and to assess the efficacy of a small-scale screening program in detecting colorectal cancer. A screening kit was requested by 1,204 individuals after hearing or reading about the program; 770 kits were returned. Of 29 (3.7%) positive test results, six were negative after retesting. Adequate follow-up was available for 18 of the remaining 23 positive results. Thirteen were due to anal bleeding, diverticulosis, or heavy ingestion of aspirin; five were considered false-positive results, since no evidence of disease could be found. No cases of polypoid tumors or carcinoma were detected. The main value of such a program in an area with a small population base is to increase the awareness of the public of the importance of routine screening for colorectal cancer.", "contents": "Screening for colorectal cancer. A voluntary colorectal screening program was carried out in North Carolina to evaluate the public's willingness to perform a do-it-yourself bowel test, to educate the public on the importance of routine colorectal screening, and to assess the efficacy of a small-scale screening program in detecting colorectal cancer. A screening kit was requested by 1,204 individuals after hearing or reading about the program; 770 kits were returned. Of 29 (3.7%) positive test results, six were negative after retesting. Adequate follow-up was available for 18 of the remaining 23 positive results. Thirteen were due to anal bleeding, diverticulosis, or heavy ingestion of aspirin; five were considered false-positive results, since no evidence of disease could be found. No cases of polypoid tumors or carcinoma were detected. The main value of such a program in an area with a small population base is to increase the awareness of the public of the importance of routine screening for colorectal cancer."}
{"id": "PMID:472842", "title": "Situs inversus and appendicitis.", "content": "Two cases of left lower quadrant appendicitis associated with situs inversus totalis are presented. Historic, genetic, and embryologic aspects of situs inversus, as well as recent theories regarding etiology are discussed. The clinical recognition of appendicitis in situs inversus is often difficult because of the unusual location of the appendix and abnormal pain localization. The differentiation of situs inversus totalis and abnormal rotation of the abdominal viscera (situs indeterminus) is important surgically because fixation of the midgut loop may be indicated in patients with situs indeterminus.", "contents": "Situs inversus and appendicitis. Two cases of left lower quadrant appendicitis associated with situs inversus totalis are presented. Historic, genetic, and embryologic aspects of situs inversus, as well as recent theories regarding etiology are discussed. The clinical recognition of appendicitis in situs inversus is often difficult because of the unusual location of the appendix and abnormal pain localization. The differentiation of situs inversus totalis and abnormal rotation of the abdominal viscera (situs indeterminus) is important surgically because fixation of the midgut loop may be indicated in patients with situs indeterminus."}
{"id": "PMID:472843", "title": "Agenesis of the gallbladder and congenital lumbar hernia.", "content": "We describe a case of congenital absence of the gallbladder and cystic duct, associated with a congenital lumbar hernia, and review the literature. Special emphasis is placed on the pathogenesis of common bile duct involvement and its effects. Treatment is aimed at correcting these primary defects and their complications.", "contents": "Agenesis of the gallbladder and congenital lumbar hernia. We describe a case of congenital absence of the gallbladder and cystic duct, associated with a congenital lumbar hernia, and review the literature. Special emphasis is placed on the pathogenesis of common bile duct involvement and its effects. Treatment is aimed at correcting these primary defects and their complications."}
{"id": "PMID:472844", "title": "(15S)-15 methyl prostaglandin F2 alpha levels in amniotic fluid and blood in second trimester abortions.", "content": "A single intra-amniotic injection of (15S)-15 methyl prostaglandin F2 alpha (THAM) was used to induce second trimester abortion in five patients. Serial levels of (15S)-15 methyl prostaglandin F2 alpha were subsequently measured in amniotic fluid and plasma by radioimmunoassay. The slow removal of this drug from the amniotic fluid was documented. Plasma levels of (15S)-15 methyl prostaglandin F2 alpha increased fourfold to sevenfold after clinical rupture of the membranes in three patients, supporting the fact that prostaglandins are well absorbed from the vagina. Because this analogue of prostaglandin can cause marked peripheral bronchoconstriction when administered systemically, it is best to avoid its use in patients with a history of asthma.", "contents": "(15S)-15 methyl prostaglandin F2 alpha levels in amniotic fluid and blood in second trimester abortions. A single intra-amniotic injection of (15S)-15 methyl prostaglandin F2 alpha (THAM) was used to induce second trimester abortion in five patients. Serial levels of (15S)-15 methyl prostaglandin F2 alpha were subsequently measured in amniotic fluid and plasma by radioimmunoassay. The slow removal of this drug from the amniotic fluid was documented. Plasma levels of (15S)-15 methyl prostaglandin F2 alpha increased fourfold to sevenfold after clinical rupture of the membranes in three patients, supporting the fact that prostaglandins are well absorbed from the vagina. Because this analogue of prostaglandin can cause marked peripheral bronchoconstriction when administered systemically, it is best to avoid its use in patients with a history of asthma."}
{"id": "PMID:472845", "title": "Relationship between spray adhesives and congenital malformations.", "content": "Evidence from clinical impressions and laboratory slides that spray adhesive products could have a potentially dangerous mutagenic or teratogenic effect was first reported in 1973. However, similar laboratory slides observed by others revealed no significant difference between chromosomal slides of malformed infants and those of normal children in households whose members were exposed to spray adhesives. Because of opposing opinions, a pilot epidemiologic study was initiated to investigate the association between the use of spray adhesives and congenital malformations. All congenital malformations reported on birth certificates from 1971 through 1974 in Oklahoma County, Oklahoma were used. A normal birth was selected as a matching control for each case. Complete and usable questionnaires regarding 112 malformed subjects and 116 controls, including 56 matched controls, were received. There was a statistical association between malformed subjects and household members using spray adhesives. There appears to be sufficient epidemiologic evidence to reopen the controversy with the appeal for further studies.", "contents": "Relationship between spray adhesives and congenital malformations. Evidence from clinical impressions and laboratory slides that spray adhesive products could have a potentially dangerous mutagenic or teratogenic effect was first reported in 1973. However, similar laboratory slides observed by others revealed no significant difference between chromosomal slides of malformed infants and those of normal children in households whose members were exposed to spray adhesives. Because of opposing opinions, a pilot epidemiologic study was initiated to investigate the association between the use of spray adhesives and congenital malformations. All congenital malformations reported on birth certificates from 1971 through 1974 in Oklahoma County, Oklahoma were used. A normal birth was selected as a matching control for each case. Complete and usable questionnaires regarding 112 malformed subjects and 116 controls, including 56 matched controls, were received. There was a statistical association between malformed subjects and household members using spray adhesives. There appears to be sufficient epidemiologic evidence to reopen the controversy with the appeal for further studies."}
{"id": "PMID:472846", "title": "Keep warm stools cold: on the status of parasitology.", "content": "Although the prevalence of parasites has decreased in some areas, no parasite has been eliminated, and the current literature indicates that parasitic diseases continue as a major cause of morbidity. In addition to endemic forms, imported parasitic diseases are being seen more frequently because of increased world travel and immigration. The high infection rates of intestinal helminths and protozoa being found in this country suggest the public is at risk of acquiring a parasitic disease. This report reviews the status of medical parasitology relative to laboratory methods and parasites prevalent in the United States.", "contents": "Keep warm stools cold: on the status of parasitology. Although the prevalence of parasites has decreased in some areas, no parasite has been eliminated, and the current literature indicates that parasitic diseases continue as a major cause of morbidity. In addition to endemic forms, imported parasitic diseases are being seen more frequently because of increased world travel and immigration. The high infection rates of intestinal helminths and protozoa being found in this country suggest the public is at risk of acquiring a parasitic disease. This report reviews the status of medical parasitology relative to laboratory methods and parasites prevalent in the United States."}
{"id": "PMID:472848", "title": "Presumed respiratory syncytial virus pneumonia in an adolescent compromised host.", "content": "A 15-year-old boy with nephrotic syndrome, renal insufficiency, and cutaneous anergy had severe pneumonia with pleural effusion. There was serologic evidence of respiratory syncytial virus infection, and extensive microbiologic, histologic, and serologic studies failed to identify any other etiologic agent. Respiratory syncytial virus as a possible cause of severe pneumonia in immunocompromised hosts has not been previously reported.", "contents": "Presumed respiratory syncytial virus pneumonia in an adolescent compromised host. A 15-year-old boy with nephrotic syndrome, renal insufficiency, and cutaneous anergy had severe pneumonia with pleural effusion. There was serologic evidence of respiratory syncytial virus infection, and extensive microbiologic, histologic, and serologic studies failed to identify any other etiologic agent. Respiratory syncytial virus as a possible cause of severe pneumonia in immunocompromised hosts has not been previously reported."}
{"id": "PMID:472849", "title": "Severe hypernatremia caused by age, isolation, and obstructive uropathy.", "content": "Although this is an extremely interesting case report from a medical viewpoint, inasmuch as it presents an unusual cause of hypernatremia, it is probably even more important because of its sociologic implications. The basic underlying causes are common in the aged, and as the number of senior and ultimately senior senior citizens increases, a repetition of the events enumerated here can be predicted in increasing numbers of patients.", "contents": "Severe hypernatremia caused by age, isolation, and obstructive uropathy. Although this is an extremely interesting case report from a medical viewpoint, inasmuch as it presents an unusual cause of hypernatremia, it is probably even more important because of its sociologic implications. The basic underlying causes are common in the aged, and as the number of senior and ultimately senior senior citizens increases, a repetition of the events enumerated here can be predicted in increasing numbers of patients."}
{"id": "PMID:472850", "title": "Acute intermittent porphyria associated with inappropriate antidiuretic hormone secretion, hypokalemic alkalosis, and secondary hyperaldosteronism.", "content": "A case of acute intermittent porphyria associated with inappropriate antidiuretic hormone secretion and secondary aldosteronism is presented. Hypokalemic alkalosis was a prominent feature of this case, and appeared to be at least partly caused by secondary aldosteronism.", "contents": "Acute intermittent porphyria associated with inappropriate antidiuretic hormone secretion, hypokalemic alkalosis, and secondary hyperaldosteronism. A case of acute intermittent porphyria associated with inappropriate antidiuretic hormone secretion and secondary aldosteronism is presented. Hypokalemic alkalosis was a prominent feature of this case, and appeared to be at least partly caused by secondary aldosteronism."}
{"id": "PMID:472851", "title": "Squamous papilloma of the esophagus.", "content": "We have described two cases of squamous papilloma of the esophagus, one of which is an unusual case of probable malignant transformation of a benign squamous papilloma to a low-grade verrucous carcinoma.", "contents": "Squamous papilloma of the esophagus. We have described two cases of squamous papilloma of the esophagus, one of which is an unusual case of probable malignant transformation of a benign squamous papilloma to a low-grade verrucous carcinoma."}
{"id": "PMID:472852", "title": "Congenital pulmonary lymphangiectasis.", "content": "We have described an unusual case of congenital pulmonary lymphangiectasis which does not conform to Felman's classification. We suggest establishing a third category in the group with non-cardiac-associated CPL, entitled \"noncardiac, intermediate onset.\"", "contents": "Congenital pulmonary lymphangiectasis. We have described an unusual case of congenital pulmonary lymphangiectasis which does not conform to Felman's classification. We suggest establishing a third category in the group with non-cardiac-associated CPL, entitled \"noncardiac, intermediate onset.\""}
{"id": "PMID:472853", "title": "Infantile Gaucher's disease.", "content": "This case report calls attention to the fact that infantile Gaucher's disease can occur in black infants. The infant's age, neurologic manifestations, and pathologic findings support the diagnosis.", "contents": "Infantile Gaucher's disease. This case report calls attention to the fact that infantile Gaucher's disease can occur in black infants. The infant's age, neurologic manifestations, and pathologic findings support the diagnosis."}
{"id": "PMID:472854", "title": "Right-sided posttraumatic diaphragmatic hernia presenting as a coin lesion.", "content": "An unusual case of right-sided posttraumatic diaphragmatic hernia is described. Ten years after closed chest trauma a 51-year-old man presented with a coin lesion at the right lung base. Thoracotomy revealed a rounded ball of liver tissue protruding through a diaphragmatic tear.", "contents": "Right-sided posttraumatic diaphragmatic hernia presenting as a coin lesion. An unusual case of right-sided posttraumatic diaphragmatic hernia is described. Ten years after closed chest trauma a 51-year-old man presented with a coin lesion at the right lung base. Thoracotomy revealed a rounded ball of liver tissue protruding through a diaphragmatic tear."}
{"id": "PMID:472855", "title": "Exacerbation of angina pectoris by prazosin.", "content": "A case of angina pectoris worsened by prazosin is presented. Since a hypertensive population includes many patients with known or occult coronary artery disease, physicians should use prazosin cautiously in this subgroup, particularly when beta-adrenergic antagonists cannot be used concurrently.", "contents": "Exacerbation of angina pectoris by prazosin. A case of angina pectoris worsened by prazosin is presented. Since a hypertensive population includes many patients with known or occult coronary artery disease, physicians should use prazosin cautiously in this subgroup, particularly when beta-adrenergic antagonists cannot be used concurrently."}
{"id": "PMID:472858", "title": "Endocarditis caused by Actinobacillus actinomycetemcomitans.", "content": "A case of endocarditis caused by Actinobacillus actinomycetemcomitans is described and 18 cases are reviewed. Clinical features of endocarditis caused by A actinomycetemcomitans and antibiotic susceptibility are reviewed. Because of the relative resistance of A actinomycetemcomitans to penicillin and ampicillin, high doses of either penicillin or ampicillin should be combined with streptomycin or gentamicin for four to six weeks.", "contents": "Endocarditis caused by Actinobacillus actinomycetemcomitans. A case of endocarditis caused by Actinobacillus actinomycetemcomitans is described and 18 cases are reviewed. Clinical features of endocarditis caused by A actinomycetemcomitans and antibiotic susceptibility are reviewed. Because of the relative resistance of A actinomycetemcomitans to penicillin and ampicillin, high doses of either penicillin or ampicillin should be combined with streptomycin or gentamicin for four to six weeks."}
{"id": "PMID:472859", "title": "Treatment of chorea gravidarum with haloperidol.", "content": "Treatment of a case of severe chorea gravidarum with haloperidol is presented. A possible neuropharmacologic explanation of the beneficial effects of the neuroleptics, particularly haloperidol, is offered based on the dopamine antagonism of these drugs. Heightened dopaminergic activity in the striatum is postulated as the basis for the chorea. Indications for the use of haloperidol in chorea gravidarum are discussed.", "contents": "Treatment of chorea gravidarum with haloperidol. Treatment of a case of severe chorea gravidarum with haloperidol is presented. A possible neuropharmacologic explanation of the beneficial effects of the neuroleptics, particularly haloperidol, is offered based on the dopamine antagonism of these drugs. Heightened dopaminergic activity in the striatum is postulated as the basis for the chorea. Indications for the use of haloperidol in chorea gravidarum are discussed."}
{"id": "PMID:472860", "title": "Giant cell arteritis in a black patient.", "content": "Giant cell arteritis has been thought to occur only in white patients. There have been several recent reports of this disorder to blacks, however, and this case of biopsy-proven giant cell arteritis in a black patient indicates the diagnosis should be considered in any elderly patient with typical symptoms regardless of race.", "contents": "Giant cell arteritis in a black patient. Giant cell arteritis has been thought to occur only in white patients. There have been several recent reports of this disorder to blacks, however, and this case of biopsy-proven giant cell arteritis in a black patient indicates the diagnosis should be considered in any elderly patient with typical symptoms regardless of race."}
{"id": "PMID:472861", "title": "Subcutaneous inflammation (infection?) after synovial rupture: a complication of a complication.", "content": "Six weeks after presumptive acute synovial rupture at the knee, a patient was admitted with cutaneous induration over the medial calf. Roentgenograms showed gas shadows within the involved area. A fluctuant area at the center of the indurated mass yielded a Corynebacterium species.", "contents": "Subcutaneous inflammation (infection?) after synovial rupture: a complication of a complication. Six weeks after presumptive acute synovial rupture at the knee, a patient was admitted with cutaneous induration over the medial calf. Roentgenograms showed gas shadows within the involved area. A fluctuant area at the center of the indurated mass yielded a Corynebacterium species."}
{"id": "PMID:472890", "title": "Anterior reduction of interlocking facets in the lower cervical spine.", "content": "Twelve patients with locked facets of the lower cervical spine were surgically treated through an anterior approach. The interlocking was bilateral in 2 cases and unilateral in 10; in all patients, skull traction failed to obtain the reduction. The technique of anterior reduction is described, with particular attention to the importance of complete removal of the disc before insertion of the distractor. Reduction was easily and safely achieved in all cases, and no significant complication occurred.", "contents": "Anterior reduction of interlocking facets in the lower cervical spine. Twelve patients with locked facets of the lower cervical spine were surgically treated through an anterior approach. The interlocking was bilateral in 2 cases and unilateral in 10; in all patients, skull traction failed to obtain the reduction. The technique of anterior reduction is described, with particular attention to the importance of complete removal of the disc before insertion of the distractor. Reduction was easily and safely achieved in all cases, and no significant complication occurred."}
{"id": "PMID:472891", "title": "Cervical spondylolisthesis.", "content": "Cervical spondylolisthesis is a rare, unusual finding in patients with neck pain. Cervical pain without neurological deficit is most often seen with this abnormality. It usually involves a defect in the pedicle of the sixth cervical vertebra. Anterior interbody cervical fusion is recommended as the treatment of choice.", "contents": "Cervical spondylolisthesis. Cervical spondylolisthesis is a rare, unusual finding in patients with neck pain. Cervical pain without neurological deficit is most often seen with this abnormality. It usually involves a defect in the pedicle of the sixth cervical vertebra. Anterior interbody cervical fusion is recommended as the treatment of choice."}
{"id": "PMID:472889", "title": "Halo-thoracic brace management of odontoid fractures.", "content": "Thirteen patients with fracture of the odontoid were treated with halo-thoracic brace. Major trauma was responsible for the fracture in only 5. Neither the degree nor the direction of displacement prevented union, which occurred in all 13. All patients were treated for least 60 days; 11 were treated longer. No complications occurred. The method is recommended based on the results.", "contents": "Halo-thoracic brace management of odontoid fractures. Thirteen patients with fracture of the odontoid were treated with halo-thoracic brace. Major trauma was responsible for the fracture in only 5. Neither the degree nor the direction of displacement prevented union, which occurred in all 13. All patients were treated for least 60 days; 11 were treated longer. No complications occurred. The method is recommended based on the results."}
{"id": "PMID:472893", "title": "Transoral cervical corpectomy with the aid of the microscope.", "content": "The transoral transpharyngeal approach to the upper cervical spine is useful in symptomatic cases of chronic atlantoaxial dislocation not reducible by other means. Spontaneous healing and fusion in such patients results in an anterior deformity or gibbus which indents the ventral surface of the spinal cord. This lesion is best approached anteriorly, with the neck in a neutral or extended position. Previously reported complications of the transoral operation, especially vertebral artery laceration, can be avoided through used of the microscope. The technique is described and 2 cases are presented. Spinal stability was not disrupted in either, and cord decompression was accomplished.", "contents": "Transoral cervical corpectomy with the aid of the microscope. The transoral transpharyngeal approach to the upper cervical spine is useful in symptomatic cases of chronic atlantoaxial dislocation not reducible by other means. Spontaneous healing and fusion in such patients results in an anterior deformity or gibbus which indents the ventral surface of the spinal cord. This lesion is best approached anteriorly, with the neck in a neutral or extended position. Previously reported complications of the transoral operation, especially vertebral artery laceration, can be avoided through used of the microscope. The technique is described and 2 cases are presented. Spinal stability was not disrupted in either, and cord decompression was accomplished."}
{"id": "PMID:472892", "title": "Intracranial aneurysm and hemorrhage following skull caliper traction. Review of skull traction complications.", "content": "Serious complications of caliper skull traction for fracture-dislocations of the cervical spine are so rare that they are not discussed in most standard textbooks. A fusiform intracranial aneurysm which followed the placement of skull tongs is reported. Subsequent aneurysmal rupture produced an intracerbral hematoma requiring drainage. The literature recording the complications of skull caliper traction is reviewed and the indications for skull traction and the need for scrupulous surgical technique are emphasized.", "contents": "Intracranial aneurysm and hemorrhage following skull caliper traction. Review of skull traction complications. Serious complications of caliper skull traction for fracture-dislocations of the cervical spine are so rare that they are not discussed in most standard textbooks. A fusiform intracranial aneurysm which followed the placement of skull tongs is reported. Subsequent aneurysmal rupture produced an intracerbral hematoma requiring drainage. The literature recording the complications of skull caliper traction is reviewed and the indications for skull traction and the need for scrupulous surgical technique are emphasized."}
{"id": "PMID:472894", "title": "Paget's disease. Reversal of severe paraparesis using calcitonin.", "content": "Relatively short-term treatment of paraparesis due to Paget's disease with subcutaneous salmon calcitonin alone produced dramatic relief of sensory loss, pain, and paraparesis. The successful outcomes in 2 patients, one with spinal cord and one with cauda equina compression, indicate a potential alternative to surgery in reversing mild-to-severe neural dysfunction in Pagets disease. The proposed mechanisms of action of calcitonin include reduction of a direct bony impingement on the neural tissue and/or a decrease of neural ischemia. It is suggested that even if paraparesis does not improve with calcitonin alone, the medication given preoperatively would probably serve a useful adjunctive role by decreasing intraoperative bone bleeding. However, those patients whose pagetic bone is already metabolically inactive would probably not benefit from calcitonin therapy.", "contents": "Paget's disease. Reversal of severe paraparesis using calcitonin. Relatively short-term treatment of paraparesis due to Paget's disease with subcutaneous salmon calcitonin alone produced dramatic relief of sensory loss, pain, and paraparesis. The successful outcomes in 2 patients, one with spinal cord and one with cauda equina compression, indicate a potential alternative to surgery in reversing mild-to-severe neural dysfunction in Pagets disease. The proposed mechanisms of action of calcitonin include reduction of a direct bony impingement on the neural tissue and/or a decrease of neural ischemia. It is suggested that even if paraparesis does not improve with calcitonin alone, the medication given preoperatively would probably serve a useful adjunctive role by decreasing intraoperative bone bleeding. However, those patients whose pagetic bone is already metabolically inactive would probably not benefit from calcitonin therapy."}
{"id": "PMID:472896", "title": "Experimentally induced hypermobility in the lumbar spine. A pathologic and radiologic study of the posterior ligament and annulus fibrosus.", "content": "Experimental destructive lesions were produced at autopsy in the lumbar discs of nine spines including 29 radiologically normal adult lumbar intervertebral discs. Limited transverse surgical division of the posterior longitudinal ligament and adjacent annular fibers were performed followed by, in addition, the removal of the intervertebral nucleus pulposus. Without interfering with any other major structure, radiologic evidence of hypermobility was recorded in 20 of 29 normal intervertebral segments. The patterns of movement were measured by standardized radiologic criteria. The available evidence suggests that localized damage to the region of the posterior longitudinal ligament and adjacent annulus fibrosus can produce radiologic patterns of hypermobility.", "contents": "Experimentally induced hypermobility in the lumbar spine. A pathologic and radiologic study of the posterior ligament and annulus fibrosus. Experimental destructive lesions were produced at autopsy in the lumbar discs of nine spines including 29 radiologically normal adult lumbar intervertebral discs. Limited transverse surgical division of the posterior longitudinal ligament and adjacent annular fibers were performed followed by, in addition, the removal of the intervertebral nucleus pulposus. Without interfering with any other major structure, radiologic evidence of hypermobility was recorded in 20 of 29 normal intervertebral segments. The patterns of movement were measured by standardized radiologic criteria. The available evidence suggests that localized damage to the region of the posterior longitudinal ligament and adjacent annulus fibrosus can produce radiologic patterns of hypermobility."}
{"id": "PMID:472895", "title": "Dynamic loads experienced in correction of idiopathic scoliosis using two types of Harrington rods.", "content": "This paper reports the results of a series of tests conducted to evaluate the dynamic loads exerted by the Harrington rod on the vertebral bones during the correction of idiopathic scoliosis. Two types of Harrington rods were tested: the standard stepped rod and a modified threaded rod. The latter had a threaded end replacing the standard stepped end. The tests were conducted on linear and nonlinear springs, the mechanical characteristics of which are similar to those previously obtained from measurements performed on human spines. The results of the tests are reported and the two rod designs are evaluated accordingly. The modified threaded rod shows definite mechanical advantages over the stepped rod; however, the design is still open for modifications to overcome certain functional difficulties. (The Custom Product Services of Zimmer Co. offers a child spinal threaded rod designed by Dr. Kenton Leatherman.)", "contents": "Dynamic loads experienced in correction of idiopathic scoliosis using two types of Harrington rods. This paper reports the results of a series of tests conducted to evaluate the dynamic loads exerted by the Harrington rod on the vertebral bones during the correction of idiopathic scoliosis. Two types of Harrington rods were tested: the standard stepped rod and a modified threaded rod. The latter had a threaded end replacing the standard stepped end. The tests were conducted on linear and nonlinear springs, the mechanical characteristics of which are similar to those previously obtained from measurements performed on human spines. The results of the tests are reported and the two rod designs are evaluated accordingly. The modified threaded rod shows definite mechanical advantages over the stepped rod; however, the design is still open for modifications to overcome certain functional difficulties. (The Custom Product Services of Zimmer Co. offers a child spinal threaded rod designed by Dr. Kenton Leatherman.)"}
{"id": "PMID:472897", "title": "Chronic venous hypertension in the tail of the Wistar rat.", "content": "Venous hypertension in the extremities has been associated with new bone formation and with degenerative joint disease. The relationship between increased intraosseous pressure and bone remodeling was investigated in the vertebrae of the tail of the Wistar rat. In 5 of 15 animals, prolonged venous hypertension resulted in osteoblastic activity with new bone formation. The vertebral changes observed are likened to those seen in some spinal degenerative diseases.", "contents": "Chronic venous hypertension in the tail of the Wistar rat. Venous hypertension in the extremities has been associated with new bone formation and with degenerative joint disease. The relationship between increased intraosseous pressure and bone remodeling was investigated in the vertebrae of the tail of the Wistar rat. In 5 of 15 animals, prolonged venous hypertension resulted in osteoblastic activity with new bone formation. The vertebral changes observed are likened to those seen in some spinal degenerative diseases."}
{"id": "PMID:472910", "title": "Ultrasound aspiration biopsy transducer amniocentesis.", "content": "Amniocentesis through an ultrasound aspiration biopsy transducer was performed 156 times. The technique is described and our results are presented. By closely monitoring the fetus and the needle entry into the amniotic cavity, we have avoided serious complications. The importance of adequate genetic counselling for the procedure is stressed, as most cases fall into the second trimester group, and the procedure is indicated in order to try to prevent inheritable disorders. The importance of continual monitoring of the fetus before, during and after the procedure is pointed out, and recommendations are made to avoid difficulties and complications in amniocentesis.", "contents": "Ultrasound aspiration biopsy transducer amniocentesis. Amniocentesis through an ultrasound aspiration biopsy transducer was performed 156 times. The technique is described and our results are presented. By closely monitoring the fetus and the needle entry into the amniotic cavity, we have avoided serious complications. The importance of adequate genetic counselling for the procedure is stressed, as most cases fall into the second trimester group, and the procedure is indicated in order to try to prevent inheritable disorders. The importance of continual monitoring of the fetus before, during and after the procedure is pointed out, and recommendations are made to avoid difficulties and complications in amniocentesis."}
{"id": "PMID:472911", "title": "The use of human chorionic gonadotropin in recurrent abortion.", "content": "Important unresolved problems associated with recurrent abortion are aetiology, prognosis and management. This communication describes hormonal monitoring in pregnancy of groups of patients treated with human chorionic gonadotropin (HCG). The outcome is compared with that in an untreated normal group and an untreated group with recurrent abortions.", "contents": "The use of human chorionic gonadotropin in recurrent abortion. Important unresolved problems associated with recurrent abortion are aetiology, prognosis and management. This communication describes hormonal monitoring in pregnancy of groups of patients treated with human chorionic gonadotropin (HCG). The outcome is compared with that in an untreated normal group and an untreated group with recurrent abortions."}
{"id": "PMID:472898", "title": "A radiologic survey of various configurations of the lumbar spine.", "content": "This paper reports an investigation to determine whether or not human spine configurations can be categorized into types by which one could predict the possibility of disablement at one specific level more than another. Configurations of the lumbar spine, the shape of the lower two lumbar discs, the anterior and posterior heights of discs, the sizes of the transverse processes of L4-5, the presence or absence of rudimentery ribs, and the presence of transitional vertebrae were studied in roentgenograms of 554 subjects. They concluded that the probable criteria for development of L4-5 degeneration were 1) high intercrestal line passing through the upper half of L4, 2) long transverse process on L5, 3) rudimentary rib, and 4) transitional vertebra. Criteria for development of L5-S1 degeneration were 1) intercrestal line passing through the body of L5, 2) short transverse process on L5, 3) no rudimentary rib, 4) no transitional vertebrae. A high intercrestal line and long transverse process probably act as antitorsional devices protecting the L5-S1 disc; hence the likelihood of degeneration at L4-5 is increased.", "contents": "A radiologic survey of various configurations of the lumbar spine. This paper reports an investigation to determine whether or not human spine configurations can be categorized into types by which one could predict the possibility of disablement at one specific level more than another. Configurations of the lumbar spine, the shape of the lower two lumbar discs, the anterior and posterior heights of discs, the sizes of the transverse processes of L4-5, the presence or absence of rudimentery ribs, and the presence of transitional vertebrae were studied in roentgenograms of 554 subjects. They concluded that the probable criteria for development of L4-5 degeneration were 1) high intercrestal line passing through the upper half of L4, 2) long transverse process on L5, 3) rudimentary rib, and 4) transitional vertebra. Criteria for development of L5-S1 degeneration were 1) intercrestal line passing through the body of L5, 2) short transverse process on L5, 3) no rudimentary rib, 4) no transitional vertebrae. A high intercrestal line and long transverse process probably act as antitorsional devices protecting the L5-S1 disc; hence the likelihood of degeneration at L4-5 is increased."}
{"id": "PMID:472912", "title": "The accuracy of maternal observation of fetal movements.", "content": "The accuracy of maternal observation of fetal movements was assessed in 20 patients during the last trimester of pregnancy. The effect of factors such as smoking, obesity, education and gestational age were correlated with maternal observations to establish the accuracy of the latter.", "contents": "The accuracy of maternal observation of fetal movements. The accuracy of maternal observation of fetal movements was assessed in 20 patients during the last trimester of pregnancy. The effect of factors such as smoking, obesity, education and gestational age were correlated with maternal observations to establish the accuracy of the latter."}
{"id": "PMID:472913", "title": "Oral and intravaginal prostaglandin E2 for cervical ripening and induction of labour.", "content": "The effect of prostaglandin E2 (PGE2) in 'ripening' the cervix was studied in 33 patients who required surgical induction of labour. Patients included primigravidas and parous subjects between 36 and 42 weeks of gestation. Sixteen patients received a total oral dose of 5 mg PGE2 and 17 received 4 - 6 mg PGE2 intravaginally in the form of a gel. There was significant improvement in 'ripeness' of the cervix in all groups, the intravaginal route giving slightly better results than the oral route. Ten patients remained unsuitable for induction because of inadequate dilatation of the cervix or a high fetal head. Four patients on oral therapy developed late decelerations of the fetal heart and 3 of these were delivered by immediate caesarean section. The implications of this and the reasons for the inability to perform inductions after 'ripening' are discussed.", "contents": "Oral and intravaginal prostaglandin E2 for cervical ripening and induction of labour. The effect of prostaglandin E2 (PGE2) in 'ripening' the cervix was studied in 33 patients who required surgical induction of labour. Patients included primigravidas and parous subjects between 36 and 42 weeks of gestation. Sixteen patients received a total oral dose of 5 mg PGE2 and 17 received 4 - 6 mg PGE2 intravaginally in the form of a gel. There was significant improvement in 'ripeness' of the cervix in all groups, the intravaginal route giving slightly better results than the oral route. Ten patients remained unsuitable for induction because of inadequate dilatation of the cervix or a high fetal head. Four patients on oral therapy developed late decelerations of the fetal heart and 3 of these were delivered by immediate caesarean section. The implications of this and the reasons for the inability to perform inductions after 'ripening' are discussed."}
{"id": "PMID:472914", "title": "Triplet pregnancy. A 10-year review of cases at Baragwanath Hospital.", "content": "A series of 61 triplet pregnancies delivered in the Baragwanath Hospital for the 10 years 1967 - 1976 is analysed, the incidence being 1 : 2 789 deliveries. The average maternal age was 29,6 years, and average parity 3,7; 60% had had adequate antenatal care, a figure significantly higher than that for the local normal pregnant population. The vast majority of the patients had vaginal deliveries, although the proportion of manipulative procedures was high. The duration of labour was strikingly short in most instances. The fetal outcome was impressively good, although somewhat better with the second triplet than with the other two. This indicates that, in spite of the recent tendency towards liberal use of caesarean sections in these patients, a careful and appropriate vaginal approach has its merits.", "contents": "Triplet pregnancy. A 10-year review of cases at Baragwanath Hospital. A series of 61 triplet pregnancies delivered in the Baragwanath Hospital for the 10 years 1967 - 1976 is analysed, the incidence being 1 : 2 789 deliveries. The average maternal age was 29,6 years, and average parity 3,7; 60% had had adequate antenatal care, a figure significantly higher than that for the local normal pregnant population. The vast majority of the patients had vaginal deliveries, although the proportion of manipulative procedures was high. The duration of labour was strikingly short in most instances. The fetal outcome was impressively good, although somewhat better with the second triplet than with the other two. This indicates that, in spite of the recent tendency towards liberal use of caesarean sections in these patients, a careful and appropriate vaginal approach has its merits."}
{"id": "PMID:472915", "title": "Pelvic dimensions and the outcome of trial labour in Shona and Zulu primigravidas.", "content": "Pelvic contraction is very prevalent in Africa while radiological facilities to assess pelvic size and shape are seldom available. A trial of labour using simple guidelines on a cervicograph can enable the midwife to detect dysfunctional labour and can help medical staff to manage these problems. Radiological checks in 116 Black primigravid patients with dysfunctional labour demonstrated that an active trial of labour is accurate in determining which of these patients have marked disproportion requiring caesarean section, minimal disproportion requiring assisted delivery, or primary uterine inertia responding to oxytocic stimulation. The role of radiological pelvimetry in African obstetrics is discussed, and the radiological features of the Shona and Zulu pelves in this series are described.", "contents": "Pelvic dimensions and the outcome of trial labour in Shona and Zulu primigravidas. Pelvic contraction is very prevalent in Africa while radiological facilities to assess pelvic size and shape are seldom available. A trial of labour using simple guidelines on a cervicograph can enable the midwife to detect dysfunctional labour and can help medical staff to manage these problems. Radiological checks in 116 Black primigravid patients with dysfunctional labour demonstrated that an active trial of labour is accurate in determining which of these patients have marked disproportion requiring caesarean section, minimal disproportion requiring assisted delivery, or primary uterine inertia responding to oxytocic stimulation. The role of radiological pelvimetry in African obstetrics is discussed, and the radiological features of the Shona and Zulu pelves in this series are described."}
{"id": "PMID:472921", "title": "The nephrotic syndrome in families.", "content": "Six families, each with 2 siblings suffering from the nephrotic syndrome, are reported. The incidence, causes, clinical picture and management of the nephrotic syndrome occurring in families are discussed, as well as possible ways of preventing this syndrome.", "contents": "The nephrotic syndrome in families. Six families, each with 2 siblings suffering from the nephrotic syndrome, are reported. The incidence, causes, clinical picture and management of the nephrotic syndrome occurring in families are discussed, as well as possible ways of preventing this syndrome."}
{"id": "PMID:472922", "title": "Tuberculous pericarditis. A review of 100 cases.", "content": "A retrospective survey of 100 Black patients with presumed tuberculous paricarditis showed that 82 presented with pericardial effusion while 18 had constrictive pericarditis. The mortality rate was 17%. Of the 82 patients with pericardial effusion, 15 developed 'constricting pericarditis' within 4 months; 12 required pericardiectomy. Sixteen patients died of cardiac tamponade; the effusion had been confirmed by a radio-isotope heart pool scan but had not been aspirated. This emphasizes the need for early and repeated pericardial aspiration. The fate of 38 rural patients with pericardial effusion was not known. Of the 18 patients with constrictive pericarditis, 7 underwent pericardiectomy, while 3 refused operation.", "contents": "Tuberculous pericarditis. A review of 100 cases. A retrospective survey of 100 Black patients with presumed tuberculous paricarditis showed that 82 presented with pericardial effusion while 18 had constrictive pericarditis. The mortality rate was 17%. Of the 82 patients with pericardial effusion, 15 developed 'constricting pericarditis' within 4 months; 12 required pericardiectomy. Sixteen patients died of cardiac tamponade; the effusion had been confirmed by a radio-isotope heart pool scan but had not been aspirated. This emphasizes the need for early and repeated pericardial aspiration. The fate of 38 rural patients with pericardial effusion was not known. Of the 18 patients with constrictive pericarditis, 7 underwent pericardiectomy, while 3 refused operation."}
{"id": "PMID:472923", "title": "Radiological features of normal and abnormal pulmonary blood flow.", "content": "A short review is given of the anatomy of the pulmonary arterial system and of the bronchial arterial system. The normal vascular pattern of the lung as well as the altered vascular pattern under a number of abnormal circumstances is described.", "contents": "Radiological features of normal and abnormal pulmonary blood flow. A short review is given of the anatomy of the pulmonary arterial system and of the bronchial arterial system. The normal vascular pattern of the lung as well as the altered vascular pattern under a number of abnormal circumstances is described."}
{"id": "PMID:472924", "title": "Psychiatric aspects of shoplifting.", "content": "Clinical evidence of depression present for some months before an episode of shoplifting without criminal intent was found in all 17 patients interviewed after the offence. The person at risk would appear to be a depressed middle-aged woman with a passive-dependent personality, married to an ineffectual rejecting husband who is unable to meet her dependency in times of crisis. Recognition of her depression and appropriate intervention are essential if a criminal deed is to be avoided.", "contents": "Psychiatric aspects of shoplifting. Clinical evidence of depression present for some months before an episode of shoplifting without criminal intent was found in all 17 patients interviewed after the offence. The person at risk would appear to be a depressed middle-aged woman with a passive-dependent personality, married to an ineffectual rejecting husband who is unable to meet her dependency in times of crisis. Recognition of her depression and appropriate intervention are essential if a criminal deed is to be avoided."}
{"id": "PMID:472925", "title": "Paroxysmal cold haemoglobinuria. A case report.", "content": "The clinical and biochemical findings in a 35-year-old Black man with paroxysmal cold haemoglobinuria are presented. Particular attention is drawn to the possible triggers of the haemolytic process, and the treatment is briefly reviewed.", "contents": "Paroxysmal cold haemoglobinuria. A case report. The clinical and biochemical findings in a 35-year-old Black man with paroxysmal cold haemoglobinuria are presented. Particular attention is drawn to the possible triggers of the haemolytic process, and the treatment is briefly reviewed."}
{"id": "PMID:472926", "title": "A stable non-prostaglandin inhibitor of platelet aggregation in human aorta intima extracts.", "content": "An inhibitor of platelet aggregation induced by adenosine diphosphate, collagen, adrenaline and thrombin is present in aorta intima extracts. The inhibitor is relatively stable and is not inactivated by prolonged incubation at 37 degrees C or 22 degrees C, but boiling for 15 seconds destroys it. It is not extracted by diethyl ether or chloroform, and is not affected by incubation with indomethacin or tranylcypromine. Platelet aggregation induced by arachidonic acid is not affected by this inhibitor. These results suggest that this inhibitor is not PGI2 (prostacyclin), a recently described potent anti-aggregatory agent generated by blood vessel microsomes.", "contents": "A stable non-prostaglandin inhibitor of platelet aggregation in human aorta intima extracts. An inhibitor of platelet aggregation induced by adenosine diphosphate, collagen, adrenaline and thrombin is present in aorta intima extracts. The inhibitor is relatively stable and is not inactivated by prolonged incubation at 37 degrees C or 22 degrees C, but boiling for 15 seconds destroys it. It is not extracted by diethyl ether or chloroform, and is not affected by incubation with indomethacin or tranylcypromine. Platelet aggregation induced by arachidonic acid is not affected by this inhibitor. These results suggest that this inhibitor is not PGI2 (prostacyclin), a recently described potent anti-aggregatory agent generated by blood vessel microsomes."}
{"id": "PMID:472927", "title": "Incidence of ovarian neoplasms at the Bloemfontein Academic Hospitals, 1972-1977.", "content": "In the Orange Free State patients with gynaecological tumours are mainly referred to the National and Pelonomi Hospitals and the Department of Oncotherapy. During the 6 years 1972-1977 there were 1 895 admissions to the gynaecological wards of the National Hospital and 9 807 admissions to those of the Pelonomi Hospital. A total of 205 cases of primary ovarian neoplasia was seen, comprising 97 Whites and 108 Blacks. These cases were further divided into benign and malignant tumours and histologically classified into four main groups. Comparisons were made between cases seen in Bloemfontein and cases at other institutions.", "contents": "Incidence of ovarian neoplasms at the Bloemfontein Academic Hospitals, 1972-1977. In the Orange Free State patients with gynaecological tumours are mainly referred to the National and Pelonomi Hospitals and the Department of Oncotherapy. During the 6 years 1972-1977 there were 1 895 admissions to the gynaecological wards of the National Hospital and 9 807 admissions to those of the Pelonomi Hospital. A total of 205 cases of primary ovarian neoplasia was seen, comprising 97 Whites and 108 Blacks. These cases were further divided into benign and malignant tumours and histologically classified into four main groups. Comparisons were made between cases seen in Bloemfontein and cases at other institutions."}
{"id": "PMID:472928", "title": "Blood bupivacaine levels in standard and segmental epidural blockade in obstetrics.", "content": "A statistically significant reduction in maternal blood bupivacaine levels could be achieved by using a segmental epidural blockade and lower concentrations of bupivacaine. Since bupivacaine has certain potential disadvantages for the mother and the fetus, its dosage should be kept as low as possible. Significantly lower blood levels without loss of analgesic quality are obtainable by using segmental epidural blockade and a lower concentration of bupivacaine.", "contents": "Blood bupivacaine levels in standard and segmental epidural blockade in obstetrics. A statistically significant reduction in maternal blood bupivacaine levels could be achieved by using a segmental epidural blockade and lower concentrations of bupivacaine. Since bupivacaine has certain potential disadvantages for the mother and the fetus, its dosage should be kept as low as possible. Significantly lower blood levels without loss of analgesic quality are obtainable by using segmental epidural blockade and a lower concentration of bupivacaine."}
{"id": "PMID:472929", "title": "The position of epidural catheters in obstetric regional anaesthesia.", "content": "The fate of plastic catheters threaded into the epidural space was studied radiographically in a total of 125 catheters in 75 patients. The longer the segment of catheter, the less likely it is to reach the intended position, because it may curl up or become deflected. The most reliable technique for accurately placing an epidural catheter at any specific level is to enter the epidural space as close as possible to that level, so that the catheter does not need to be threaded for more than 2--3 cm.", "contents": "The position of epidural catheters in obstetric regional anaesthesia. The fate of plastic catheters threaded into the epidural space was studied radiographically in a total of 125 catheters in 75 patients. The longer the segment of catheter, the less likely it is to reach the intended position, because it may curl up or become deflected. The most reliable technique for accurately placing an epidural catheter at any specific level is to enter the epidural space as close as possible to that level, so that the catheter does not need to be threaded for more than 2--3 cm."}
{"id": "PMID:472930", "title": "Migration of a particulate radioactive tracer from the vagina to the peritoneal cavity and ovaries.", "content": "In this report we describe a radionuclide procedure designed to evaluate the migration of a particulate radioactive tracer from the vagina to the peritoneal cavity and ovaries, as well as the determination of the patency of the pathways between these two extremes of the female reproductive system. 99mTc-labelled human albumin microspheres (99mTc-HAM) were deposited in the posterior fornices of 24 patients a day before they were to undergo different gynaecological operations. During this period sequential images were obtained and after the operation radioactivity levels in the removed organs and tissues were counted with a scintillation detector. In 14 out of 21 cases, the ovaries and fallopian tubes were counted separately from the uterus. Nine were positive (radioactivity levels were sufficiently high in the tubes and ovaries) and 5 were negative (no substantial radioactivity levels could be detected in either the tubes or the ovaries). The 5 negative results all occurred in patients with proved tubal damage as a result of previous infection. All the results were either true positive or true negative, providing evidence of migration, or obstruction, of 99mTc-HAM from the vagina through the uterus and tubes to the peritoneal cavity and ovaries.", "contents": "Migration of a particulate radioactive tracer from the vagina to the peritoneal cavity and ovaries. In this report we describe a radionuclide procedure designed to evaluate the migration of a particulate radioactive tracer from the vagina to the peritoneal cavity and ovaries, as well as the determination of the patency of the pathways between these two extremes of the female reproductive system. 99mTc-labelled human albumin microspheres (99mTc-HAM) were deposited in the posterior fornices of 24 patients a day before they were to undergo different gynaecological operations. During this period sequential images were obtained and after the operation radioactivity levels in the removed organs and tissues were counted with a scintillation detector. In 14 out of 21 cases, the ovaries and fallopian tubes were counted separately from the uterus. Nine were positive (radioactivity levels were sufficiently high in the tubes and ovaries) and 5 were negative (no substantial radioactivity levels could be detected in either the tubes or the ovaries). The 5 negative results all occurred in patients with proved tubal damage as a result of previous infection. All the results were either true positive or true negative, providing evidence of migration, or obstruction, of 99mTc-HAM from the vagina through the uterus and tubes to the peritoneal cavity and ovaries."}
{"id": "PMID:472931", "title": "A krypton-81m generator for organ ventilation and perfusion investigations.", "content": "A sterile pyrogen-free generator for ultra-short half-life 81mKr has been developed. During initial loading, the binding efficiency of 81Rb is 90--99%. The count rate during perfusion is 3 x 10(5) counts per minute for a 1,9 GBq (50 mCi) 81Rb generator. The generator has been used for routine and special investigations of lung perfusion and ventilation and for myocardial perfusion during catheterization.", "contents": "A krypton-81m generator for organ ventilation and perfusion investigations. A sterile pyrogen-free generator for ultra-short half-life 81mKr has been developed. During initial loading, the binding efficiency of 81Rb is 90--99%. The count rate during perfusion is 3 x 10(5) counts per minute for a 1,9 GBq (50 mCi) 81Rb generator. The generator has been used for routine and special investigations of lung perfusion and ventilation and for myocardial perfusion during catheterization."}
{"id": "PMID:472932", "title": "Folate metabolism and in vivo radiofolate binding in normal, folate-deficient and folate-saturated subjects.", "content": "After ingestion of 14C-CH3H4Pte Glu by folate-deficient and folate-saturated subjects, and 14C-CH3H4PteGlu as well as 3H-PteGlu by a normal control subject, the dialysis-resistant (bound) plasma radiofolate fraction appeared increased in folate saturation and decreased in folate deficiency (compared with normal folate status). This suggests that absorbed radiofolate does not admix with the total folate pool before complexing with the plasma binder. As the bound plasma fractions appeared later than did the total biofolate peaks, in vivo plasma folate binding probably occurs independently of the intestinal folate absorption process. It also appears unrelated to postabsorption storage folate displacement, as this biofolate fraction is unbound. A bound radiofolate fraction persisting in plasma for 72 hours in spite of a normal food intake indicates a relatively inert binder complex.", "contents": "Folate metabolism and in vivo radiofolate binding in normal, folate-deficient and folate-saturated subjects. After ingestion of 14C-CH3H4Pte Glu by folate-deficient and folate-saturated subjects, and 14C-CH3H4PteGlu as well as 3H-PteGlu by a normal control subject, the dialysis-resistant (bound) plasma radiofolate fraction appeared increased in folate saturation and decreased in folate deficiency (compared with normal folate status). This suggests that absorbed radiofolate does not admix with the total folate pool before complexing with the plasma binder. As the bound plasma fractions appeared later than did the total biofolate peaks, in vivo plasma folate binding probably occurs independently of the intestinal folate absorption process. It also appears unrelated to postabsorption storage folate displacement, as this biofolate fraction is unbound. A bound radiofolate fraction persisting in plasma for 72 hours in spite of a normal food intake indicates a relatively inert binder complex."}
{"id": "PMID:472933", "title": "[Appendicitis in the Black population].", "content": "In this retrospective study important aspects of appendicitis in 252 Blacks were studied and some interesting conclusions were drawn from clinical and operative data. Certain widely accepted impressions and beliefs are substantiated and others are refuted by the evidence found. In 38% of patients see, symptoms were present for more than 4 days before admission, but the clinical picture corresponded remarkably well with the textbook description. Operative findings revealed a diagnostic error rate of 18%, and the eventual mortality of 2% was confined to the 1st and 5th decades.", "contents": "[Appendicitis in the Black population]. In this retrospective study important aspects of appendicitis in 252 Blacks were studied and some interesting conclusions were drawn from clinical and operative data. Certain widely accepted impressions and beliefs are substantiated and others are refuted by the evidence found. In 38% of patients see, symptoms were present for more than 4 days before admission, but the clinical picture corresponded remarkably well with the textbook description. Operative findings revealed a diagnostic error rate of 18%, and the eventual mortality of 2% was confined to the 1st and 5th decades."}
{"id": "PMID:472934", "title": "Knee ligament injuries.", "content": "Since acute surgical repair is vastly superior to any form of reconstructive procedure available, it is extremely important that acute ligamentous injuries of the knee do not go unrecognized. Chronic symptomatic instability after untreated major ligament injury marks \"the beginning of the end' for the knee, and reconstruction is indicated. The classical stress tests for knee ligament disruption are generally reliable, and the interested doctor should have little difficulty in accurately diagnosing and classifying acute injuries or chronic instability. The technique of examination and classification of knee instability is described. The techniques of acute repair, as well as of selected reconstructive procedures, are described. Reconstruction for anteromedial subluxation is confined to advancement of the medial collateral ligament and posteromedial capsule as a single cuff, moving its tibial attachment distally and anteriorly. Fascia lata reconstruction is used for repair of anterolateral subluxation. Both these extra-articular anterior reconstructions can, in severe cases, be improved by the addition of a midline procedure, using the patellar tendon as an intra-articular graft. Transfer of the medial head of the gastrocnemius muscle is used for symptomatic posterior subluxation.", "contents": "Knee ligament injuries. Since acute surgical repair is vastly superior to any form of reconstructive procedure available, it is extremely important that acute ligamentous injuries of the knee do not go unrecognized. Chronic symptomatic instability after untreated major ligament injury marks \"the beginning of the end' for the knee, and reconstruction is indicated. The classical stress tests for knee ligament disruption are generally reliable, and the interested doctor should have little difficulty in accurately diagnosing and classifying acute injuries or chronic instability. The technique of examination and classification of knee instability is described. The techniques of acute repair, as well as of selected reconstructive procedures, are described. Reconstruction for anteromedial subluxation is confined to advancement of the medial collateral ligament and posteromedial capsule as a single cuff, moving its tibial attachment distally and anteriorly. Fascia lata reconstruction is used for repair of anterolateral subluxation. Both these extra-articular anterior reconstructions can, in severe cases, be improved by the addition of a midline procedure, using the patellar tendon as an intra-articular graft. Transfer of the medial head of the gastrocnemius muscle is used for symptomatic posterior subluxation."}
{"id": "PMID:472935", "title": "Myositis ossificans progressiva. A case report.", "content": "The clinical and laboratory features of a case of myositis ossificans progressiva are described, and the recent literature concerning the pathogenesis and treatment of the condition is briefly reviewed.", "contents": "Myositis ossificans progressiva. A case report. The clinical and laboratory features of a case of myositis ossificans progressiva are described, and the recent literature concerning the pathogenesis and treatment of the condition is briefly reviewed."}
{"id": "PMID:472936", "title": "Pituitary fossa volume in primary hypothyroidism.", "content": "Pituitary enlargement can occur in primary hypothyroidism. Pituitary volume determination in 20 cases of primary hypothyroidism showed pituitary fossa enlargement in 25% of the patients, with close correlation to highest serum thyrotropin (TSH) levels. Normalization of TSH serum levels should prevent hypertrophy of the thyrotropic cells.", "contents": "Pituitary fossa volume in primary hypothyroidism. Pituitary enlargement can occur in primary hypothyroidism. Pituitary volume determination in 20 cases of primary hypothyroidism showed pituitary fossa enlargement in 25% of the patients, with close correlation to highest serum thyrotropin (TSH) levels. Normalization of TSH serum levels should prevent hypertrophy of the thyrotropic cells."}
{"id": "PMID:472937", "title": "Pseudo-iniencephaly. A case report.", "content": "A case of congenital webbing with other congenital abnormalities is presented. Initial examination of the features of the case made a diagnosis of iniencephaly seem likely. However, further examination of the radiographs and the physical abnormalities demonstrated too few of the typical features, and the term pseudo-iniencephaly has been used to describe the case. The various aetiological possibilities are discussed, but it is clear that further research into webbing and its concomitant abnormalities would be necessary to provide the answers to the questions posed by this case.", "contents": "Pseudo-iniencephaly. A case report. A case of congenital webbing with other congenital abnormalities is presented. Initial examination of the features of the case made a diagnosis of iniencephaly seem likely. However, further examination of the radiographs and the physical abnormalities demonstrated too few of the typical features, and the term pseudo-iniencephaly has been used to describe the case. The various aetiological possibilities are discussed, but it is clear that further research into webbing and its concomitant abnormalities would be necessary to provide the answers to the questions posed by this case."}
{"id": "PMID:472938", "title": "[Ophthalmomyiasis with subretinal tracks. A case report].", "content": "A 12-year-old White Boy presented with unilateral loss of vision and a severe vitreal inflammatory reaction after he had been struck in the eye by a flying object. After treatment with corticosteroids the vitreous cleared and the fundus could be seen. Indirect ophthalmoscopic examination and fluorescein angiography showed subretinal tracks consistent with the diagnosis of ophthalmomyiasis posterior.", "contents": "[Ophthalmomyiasis with subretinal tracks. A case report]. A 12-year-old White Boy presented with unilateral loss of vision and a severe vitreal inflammatory reaction after he had been struck in the eye by a flying object. After treatment with corticosteroids the vitreous cleared and the fundus could be seen. Indirect ophthalmoscopic examination and fluorescein angiography showed subretinal tracks consistent with the diagnosis of ophthalmomyiasis posterior."}
{"id": "PMID:472939", "title": "Selective arterial embolization of the brain in an inoperable arteriovenous malformation in the thalamus. A case report.", "content": "The embolization of an inoperable arteriovenous malformation of the brain using gelatin foam strips via the Seldinger technique is described. Subsequent operative ligation of the major feeding vessels was performed, with an excellent postoperative result.", "contents": "Selective arterial embolization of the brain in an inoperable arteriovenous malformation in the thalamus. A case report. The embolization of an inoperable arteriovenous malformation of the brain using gelatin foam strips via the Seldinger technique is described. Subsequent operative ligation of the major feeding vessels was performed, with an excellent postoperative result."}
{"id": "PMID:472947", "title": "Chlorambucil therapy in childhood nephrotic syndrome.", "content": "Chlorambucil (Leukeran), a cytotoxic agent, was administered to 13 children with the nephrotic syndrome who had responded to steroid therapy, but frequently relapsed. In all patients corticosteroid therapy had become unsatisfactory. Eleven patients have so far remained in remission for an average follow-up period of 31 months since chlorambucil therapy. No side-effects of therapy were observed in this study, but several grave complications of high-dosage therapy have been reported in the recent literature. A dose of 0.2 mg/kg/d for 8 weeks should not be exceeded.", "contents": "Chlorambucil therapy in childhood nephrotic syndrome. Chlorambucil (Leukeran), a cytotoxic agent, was administered to 13 children with the nephrotic syndrome who had responded to steroid therapy, but frequently relapsed. In all patients corticosteroid therapy had become unsatisfactory. Eleven patients have so far remained in remission for an average follow-up period of 31 months since chlorambucil therapy. No side-effects of therapy were observed in this study, but several grave complications of high-dosage therapy have been reported in the recent literature. A dose of 0.2 mg/kg/d for 8 weeks should not be exceeded."}
{"id": "PMID:472948", "title": "Smoking behaviour of White, Black, Coloured and Indian South Africans. Some statistical data on a major public health hazard.", "content": "An analysis of the smoking habits of the population groups in the Republic of South Africa indicates an alarmingly high incidence of smoking, particularly among Coloureds. Significant numbers of men and women in all the population groups, especially Whites, are former smokers.", "contents": "Smoking behaviour of White, Black, Coloured and Indian South Africans. Some statistical data on a major public health hazard. An analysis of the smoking habits of the population groups in the Republic of South Africa indicates an alarmingly high incidence of smoking, particularly among Coloureds. Significant numbers of men and women in all the population groups, especially Whites, are former smokers."}
{"id": "PMID:472949", "title": "Bronchogenic carcinoma with coexisting active pulmonary tuberculosis in urban Blacks.", "content": "There is a significant incidence of active pulmonary tuberculosis with coexisting lung cancer. It is likely that the cancer activates dormant tuberculosis. In a population group beset by a high incidence of tuberculosis, the heavy smoker who has attained his fifth decade seems particularly vulnerable to the combined diseases.", "contents": "Bronchogenic carcinoma with coexisting active pulmonary tuberculosis in urban Blacks. There is a significant incidence of active pulmonary tuberculosis with coexisting lung cancer. It is likely that the cancer activates dormant tuberculosis. In a population group beset by a high incidence of tuberculosis, the heavy smoker who has attained his fifth decade seems particularly vulnerable to the combined diseases."}
{"id": "PMID:472950", "title": "Postoperative Clostridium septicum dermatitis simulating toxic epidermal necrolysis.", "content": "Widespread detachment of the epidermis as a result of Clostridium septicum infection after a laparotomy for intestinal obstruction is described. A clinicopathological picture, hitherto undescribed, is outlined.", "contents": "Postoperative Clostridium septicum dermatitis simulating toxic epidermal necrolysis. Widespread detachment of the epidermis as a result of Clostridium septicum infection after a laparotomy for intestinal obstruction is described. A clinicopathological picture, hitherto undescribed, is outlined."}
{"id": "PMID:472951", "title": "Actinic chickenpox. Light-distributed varicella eruption.", "content": "When areas of the skin which are normally covered become sunburnt just before chickenpox eruption, there occurs a marked intensification of the rash on the freshly burnt parts. This phenomenon is not widely known. Its history, published reports and our original observations on this 'actinic' chickenpox are presented, with comment on its pathogenesis.", "contents": "Actinic chickenpox. Light-distributed varicella eruption. When areas of the skin which are normally covered become sunburnt just before chickenpox eruption, there occurs a marked intensification of the rash on the freshly burnt parts. This phenomenon is not widely known. Its history, published reports and our original observations on this 'actinic' chickenpox are presented, with comment on its pathogenesis."}
{"id": "PMID:472952", "title": "The effect of long-term use of cimetidine on the ultrastructure of gastric parietal cells in man.", "content": "The effect of long-term cimetidine therapy on the ultrastructure of human parietal cells was investigated in 2 subjects. It was found that after cimetidine therapy for 1 year: (i) the ultrastructural appearance of the parietal cells was normal, and (ii) the parietal cells responded normally to stimulation by pentagastrin. Evidence in favour of a membrane flow mechanism involved in acid secretion is presented.", "contents": "The effect of long-term use of cimetidine on the ultrastructure of gastric parietal cells in man. The effect of long-term cimetidine therapy on the ultrastructure of human parietal cells was investigated in 2 subjects. It was found that after cimetidine therapy for 1 year: (i) the ultrastructural appearance of the parietal cells was normal, and (ii) the parietal cells responded normally to stimulation by pentagastrin. Evidence in favour of a membrane flow mechanism involved in acid secretion is presented."}
{"id": "PMID:472953", "title": "The radiological features of primary pulmonary tuberculosis.", "content": "A very brief review is given of the pathology of primary pulmonary tuberculosis. Numerous characteristic, but not pathognomonic, radiological appearances of primary pulmonary tuberculosis are briefly described and some of them are illustrated. The final diagnosis, however, depends on correlating the radiological picture with the clinical picture and special investigations.", "contents": "The radiological features of primary pulmonary tuberculosis. A very brief review is given of the pathology of primary pulmonary tuberculosis. Numerous characteristic, but not pathognomonic, radiological appearances of primary pulmonary tuberculosis are briefly described and some of them are illustrated. The final diagnosis, however, depends on correlating the radiological picture with the clinical picture and special investigations."}
{"id": "PMID:472961", "title": "Yersinia enterocolitica biotype 1 in South Africa.", "content": "Two clinical isolates of Yersinia enterocolitica biotype 1, positive for indole and lecithinase production, xylose and salicin fermentation, and aesculin hydrolysis, are reported. One strain, serotype 0 6, was recovered from a healthy food handler, while the other, serotype 0 5, was associated with a diarrhoeal illness in a 1-year-old child. This is the first report of either of these serotypes from human material in South Africa.", "contents": "Yersinia enterocolitica biotype 1 in South Africa. Two clinical isolates of Yersinia enterocolitica biotype 1, positive for indole and lecithinase production, xylose and salicin fermentation, and aesculin hydrolysis, are reported. One strain, serotype 0 6, was recovered from a healthy food handler, while the other, serotype 0 5, was associated with a diarrhoeal illness in a 1-year-old child. This is the first report of either of these serotypes from human material in South Africa."}
{"id": "PMID:472962", "title": "Antibacterial activity of amniotic fluid.", "content": "The antibacterial activity of amniotic fluid against Escherichia coli in the last month of pregnancy was reduced in an unselected sample of mothers delivering in Cape Town. Amniotic fluid zinc, required for normal antibacterial activity, was also reduced. These findings offer an explanation for the high incidence of amniotic fluid infection previously reported.", "contents": "Antibacterial activity of amniotic fluid. The antibacterial activity of amniotic fluid against Escherichia coli in the last month of pregnancy was reduced in an unselected sample of mothers delivering in Cape Town. Amniotic fluid zinc, required for normal antibacterial activity, was also reduced. These findings offer an explanation for the high incidence of amniotic fluid infection previously reported."}
{"id": "PMID:472963", "title": "Creatine phosphokinase and lactate dehydrogenase levels after ultra long-distance running. An analysis of iso-enzyme profiles with special reference to indicators of myocardial damage.", "content": "Blood samples obtained from 20 runners on completion or partial completion of a 160-km marathon were assayed for total serum creatine kinase (CK) and lactate dehydrogenase (LD), as well as for the respective iso-enzymes. Total CK was significantly elevated in all subjects, the mean value (2 243 U/l) constituting a 24-fold increase above the normal upper limit. In 65% of these cases, the cardiac-specific iso-enzyme of CK, MB, was present. The mean LD level (1 220 U/l) also exceeded the upper limit of normal significantly, 5 subjects presenting a 'flipped' LD pattern (LD1 greater than LD2). Three subjects exhibited the MB iso-enzyme in the presence of a flipped LD pattern. No electrocardiographic evidence of irreversible cardiac 'damage' could, however, be established after the race, nor any significant decrease in the Bruce protocol stress test rating. It is concluded that the MB iso-enzyme of CK, even in conjunction with a flipped LD pattern, may not represent unequivocal evidence of myocardial damage under all circumstances.", "contents": "Creatine phosphokinase and lactate dehydrogenase levels after ultra long-distance running. An analysis of iso-enzyme profiles with special reference to indicators of myocardial damage. Blood samples obtained from 20 runners on completion or partial completion of a 160-km marathon were assayed for total serum creatine kinase (CK) and lactate dehydrogenase (LD), as well as for the respective iso-enzymes. Total CK was significantly elevated in all subjects, the mean value (2 243 U/l) constituting a 24-fold increase above the normal upper limit. In 65% of these cases, the cardiac-specific iso-enzyme of CK, MB, was present. The mean LD level (1 220 U/l) also exceeded the upper limit of normal significantly, 5 subjects presenting a 'flipped' LD pattern (LD1 greater than LD2). Three subjects exhibited the MB iso-enzyme in the presence of a flipped LD pattern. No electrocardiographic evidence of irreversible cardiac 'damage' could, however, be established after the race, nor any significant decrease in the Bruce protocol stress test rating. It is concluded that the MB iso-enzyme of CK, even in conjunction with a flipped LD pattern, may not represent unequivocal evidence of myocardial damage under all circumstances."}
{"id": "PMID:472964", "title": "Absorption of iron polymaltose and ferrous sulphate in rats and humans. A comparative study.", "content": "Free iron in the circulation in contrast to iron complexed with protein or carbohyrdate, is intensely toxic. The annual morbidity and mortality from accidental overdose with iron salts is significant and has directed attention to the evaluation of safer forms of therapy. Comparative studies between oral ferrous sulphate and iron polymaltose in rats established an LD100 of 350 mg/kg for the salt, whereas neither morbidity nor mortality could be produced in doses exceeding 1 000 mg/kg for the complex. Bio-availability of iron from salt and complex for haemoglobin synthesis was then compared both in rats and in man with a twin-isotope technique. In experimental animals and in the human studies, venesected individuals were used to reproduce the iron deficiency state where treatment would be indicated. It was concluded that bioavailability is comparable for therapeutic doses of ferrous sulphate and iron polymaltose in iron-deficient subjects. The marginally lower absorption of complex was offset by a greater degree of gastrointestinal tract tolerance; the wide margin of safety may be of importance in reducing the danger of accidental iron overdose.", "contents": "Absorption of iron polymaltose and ferrous sulphate in rats and humans. A comparative study. Free iron in the circulation in contrast to iron complexed with protein or carbohyrdate, is intensely toxic. The annual morbidity and mortality from accidental overdose with iron salts is significant and has directed attention to the evaluation of safer forms of therapy. Comparative studies between oral ferrous sulphate and iron polymaltose in rats established an LD100 of 350 mg/kg for the salt, whereas neither morbidity nor mortality could be produced in doses exceeding 1 000 mg/kg for the complex. Bio-availability of iron from salt and complex for haemoglobin synthesis was then compared both in rats and in man with a twin-isotope technique. In experimental animals and in the human studies, venesected individuals were used to reproduce the iron deficiency state where treatment would be indicated. It was concluded that bioavailability is comparable for therapeutic doses of ferrous sulphate and iron polymaltose in iron-deficient subjects. The marginally lower absorption of complex was offset by a greater degree of gastrointestinal tract tolerance; the wide margin of safety may be of importance in reducing the danger of accidental iron overdose."}
{"id": "PMID:472976", "title": "Rehabilitation after mastectomy: the group process.", "content": "Mastectomy is a frequently performed, emotionally stressful surgical procedure. Based on theories of loss and grief and crisis intervention, an in-hospital group program has been established to facilitate rehabilitation of patient and family. This paper discusses the alleviation of grief reactions and the fostering of growth-producing behavior through early postoperative participation in such groups. The function of the social worker as a member of an interdisciplinary team is examined, with particular focus on skills involved in promoting and enhancing the group process.", "contents": "Rehabilitation after mastectomy: the group process. Mastectomy is a frequently performed, emotionally stressful surgical procedure. Based on theories of loss and grief and crisis intervention, an in-hospital group program has been established to facilitate rehabilitation of patient and family. This paper discusses the alleviation of grief reactions and the fostering of growth-producing behavior through early postoperative participation in such groups. The function of the social worker as a member of an interdisciplinary team is examined, with particular focus on skills involved in promoting and enhancing the group process."}
{"id": "PMID:472977", "title": "Social work practice and chronic pediatric illness.", "content": "The role of social workers in a pediatric chronic illness setting is presented. The difference between acute and chronic illness is explored, with emphasis on the ramifications of these differences for social work practice. Early assessment of patients and families provides the social worker with a data base from which to select an appropriate intervention with families. Of primary importance is the amelioration of feelings of helplessness, dependency, and isolation. This may be accomplished by helping families anticipate problems, mobilizing resources to help, and encouraging families to share their experiences with other families. Because the medical system, particularly  in a teaching hospital, may not provide continuity of care for patients, the relationship of the social worker to patient and family, and resulting knowledge of the family, becomes an important resource in the provision of high-quality care.", "contents": "Social work practice and chronic pediatric illness. The role of social workers in a pediatric chronic illness setting is presented. The difference between acute and chronic illness is explored, with emphasis on the ramifications of these differences for social work practice. Early assessment of patients and families provides the social worker with a data base from which to select an appropriate intervention with families. Of primary importance is the amelioration of feelings of helplessness, dependency, and isolation. This may be accomplished by helping families anticipate problems, mobilizing resources to help, and encouraging families to share their experiences with other families. Because the medical system, particularly  in a teaching hospital, may not provide continuity of care for patients, the relationship of the social worker to patient and family, and resulting knowledge of the family, becomes an important resource in the provision of high-quality care."}
{"id": "PMID:472978", "title": "Group psychotherapy on a children's urology service.", "content": "This paper describes an ongoing program, in a large metropolitan hospital, of weekly group meetings for hospitalized children on the Babies Hospital Urology Service, led by a pediatric psychiatrist and a psychiatric social worker. The purpose of the program is to help children cope with the multitude of fears and anxieties that can arise in the course of hospitalization. Although not described in detail, corollary weekly group meetings are also held with parents of the children. The authors describe the format of their meetings with child patient groups, whose composition is, by its nature, a constantly changing one. Examples are excerpted from typical group sessions, indicating the kinds of problems that surfaced and how they were dealt with. Ways in which group interaction and support contributed to the positive results of such meetings are also shown. The authors suggest that their program can be utilized in a variety of hospital settings.", "contents": "Group psychotherapy on a children's urology service. This paper describes an ongoing program, in a large metropolitan hospital, of weekly group meetings for hospitalized children on the Babies Hospital Urology Service, led by a pediatric psychiatrist and a psychiatric social worker. The purpose of the program is to help children cope with the multitude of fears and anxieties that can arise in the course of hospitalization. Although not described in detail, corollary weekly group meetings are also held with parents of the children. The authors describe the format of their meetings with child patient groups, whose composition is, by its nature, a constantly changing one. Examples are excerpted from typical group sessions, indicating the kinds of problems that surfaced and how they were dealt with. Ways in which group interaction and support contributed to the positive results of such meetings are also shown. The authors suggest that their program can be utilized in a variety of hospital settings."}
{"id": "PMID:472979", "title": "An interdisciplinary health care setting's experience with groups for parents of children having specific disabilities.", "content": "This paper describes the planning, implementation, use, and evaluation of a group for parents of children with Williams elfin facies syndrome in an interdisciplinary health care setting. The group utilized a modified educational approach that resulted in significant gains reported by participants in learning about educational opportunities and rights of the handicapped, terminology and labeling of Williams syndrome, and meeting and sharing with other parents. The experience has implications applicable in interdisciplinary health care settings for use of groups as an aid in reducing the conflict that may result from the frequent combination of research and service to individuals with specific disabilities.", "contents": "An interdisciplinary health care setting's experience with groups for parents of children having specific disabilities. This paper describes the planning, implementation, use, and evaluation of a group for parents of children with Williams elfin facies syndrome in an interdisciplinary health care setting. The group utilized a modified educational approach that resulted in significant gains reported by participants in learning about educational opportunities and rights of the handicapped, terminology and labeling of Williams syndrome, and meeting and sharing with other parents. The experience has implications applicable in interdisciplinary health care settings for use of groups as an aid in reducing the conflict that may result from the frequent combination of research and service to individuals with specific disabilities."}
{"id": "PMID:472980", "title": "The integration of services of a medical clinic and a community mental health center in a rural area.", "content": "This article describes the authors' use of open systems theory and exchange theory as a model for integrating the services to clients by the Community Medical Group (a private medical clinic) and the Mississippi River Human Services Center (a federally funded public community mental health center). As the directors of their two programs, the authors specifically patterned the integration of their services after the systems theory model of inputs, processing, and outputs. Expensive duplication of services was avoided, and the two programs worked together to develop needed programs to serve the emotionally troubled, the alcohol/drug abuser, and the developmentally disabled in their service area.", "contents": "The integration of services of a medical clinic and a community mental health center in a rural area. This article describes the authors' use of open systems theory and exchange theory as a model for integrating the services to clients by the Community Medical Group (a private medical clinic) and the Mississippi River Human Services Center (a federally funded public community mental health center). As the directors of their two programs, the authors specifically patterned the integration of their services after the systems theory model of inputs, processing, and outputs. Expensive duplication of services was avoided, and the two programs worked together to develop needed programs to serve the emotionally troubled, the alcohol/drug abuser, and the developmentally disabled in their service area."}
{"id": "PMID:472981", "title": "Providing health and social services to illegal alien families: a dilemma for community agencies.", "content": "Proposals for dealing with illegeal migration from Mexico to the United States generally do not recognize it as an international social problem. The proposals also present contradictory solutions. Amnesty, a humanitarian policy, is being suggested as well as increased restrictions and punishments, a policing policy. However, in the absence of a comprehensive national policy, community social and health care programs must provide services to illegal aliens. This article attempts to document some of the issues that illegal immigration presents for community agencies.", "contents": "Providing health and social services to illegal alien families: a dilemma for community agencies. Proposals for dealing with illegeal migration from Mexico to the United States generally do not recognize it as an international social problem. The proposals also present contradictory solutions. Amnesty, a humanitarian policy, is being suggested as well as increased restrictions and punishments, a policing policy. However, in the absence of a comprehensive national policy, community social and health care programs must provide services to illegal aliens. This article attempts to document some of the issues that illegal immigration presents for community agencies."}
{"id": "PMID:472982", "title": "Typologies in social work practice.", "content": "Two examples of diagnostic-intervention typologies are presented as derived from case record review. A multidimensional classification combines the origin, location, and identification (via family diagnosis) of children's \"behavior problems\" with treatment strategies for each of the six types. In the second example, a prescriptive typology of families with a newly diagnosed retarded child draws upon family and crisis theories to help define problem areas of family functioning. The typologies are suggested as tools but not stereotypes for clinical social work practice.", "contents": "Typologies in social work practice. Two examples of diagnostic-intervention typologies are presented as derived from case record review. A multidimensional classification combines the origin, location, and identification (via family diagnosis) of children's \"behavior problems\" with treatment strategies for each of the six types. In the second example, a prescriptive typology of families with a newly diagnosed retarded child draws upon family and crisis theories to help define problem areas of family functioning. The typologies are suggested as tools but not stereotypes for clinical social work practice."}
{"id": "PMID:472991", "title": "Small intestine cutaneous fistulas.", "content": "A retrospective study of 174 patients with small intestine cutaneous fistulas was carried out. Cause, location of fistula, amount of output, presence of inflammation, intestinal obstruction, presence of malnutrition and anemia are associated wih an increased mortality. The results of various modes of treatment indicate that intestinal resection with primary anastomosis, carried out six weeks or later after the onset of the fistula, was associated with the lowest mortality and the highest success rate.", "contents": "Small intestine cutaneous fistulas. A retrospective study of 174 patients with small intestine cutaneous fistulas was carried out. Cause, location of fistula, amount of output, presence of inflammation, intestinal obstruction, presence of malnutrition and anemia are associated wih an increased mortality. The results of various modes of treatment indicate that intestinal resection with primary anastomosis, carried out six weeks or later after the onset of the fistula, was associated with the lowest mortality and the highest success rate."}
{"id": "PMID:472992", "title": "Circulatory effects of endotoxin on unanesthetized sheep and the response to dopamine.", "content": "The cardiovascular effects of endotoxin in the unanesthetized chronically instrumented sheep were investigated. The observations were extended to include the response to intravenous infusions of dopamine to the same sheep at the height of endotoxin action. When the cardiovascular effects of endotoxin in the unanesthetized and anesthetized sheep were compared, there were distinct differences. Unanesthetized sheep tolerated considerably greater dosages of endotoxin. In anesthetized sheep, the compensatory stimulation of the sympathoadrenal system seems to have been blunted and all of the circulatory parameters deteriorate rapidly, ending in death within three hours. In the absence of anesthesia, there was clear evidence of a compensatory sympathoadrenal stimulation, as reflected by the return of most of the circulatory parameters to normal values, and furthermore, the sheep recovered from the effects of endotoxin. The exact mechanism by which anesthesia altered the response of the sheep to endotoxin was not clear. Depression of the baroreceptor and neurohumoral compensatory mechanisms was suggested. The present data indicated that dopamine was a useful agent in correcting the cardiovascular changes brought about by endotoxin.", "contents": "Circulatory effects of endotoxin on unanesthetized sheep and the response to dopamine. The cardiovascular effects of endotoxin in the unanesthetized chronically instrumented sheep were investigated. The observations were extended to include the response to intravenous infusions of dopamine to the same sheep at the height of endotoxin action. When the cardiovascular effects of endotoxin in the unanesthetized and anesthetized sheep were compared, there were distinct differences. Unanesthetized sheep tolerated considerably greater dosages of endotoxin. In anesthetized sheep, the compensatory stimulation of the sympathoadrenal system seems to have been blunted and all of the circulatory parameters deteriorate rapidly, ending in death within three hours. In the absence of anesthesia, there was clear evidence of a compensatory sympathoadrenal stimulation, as reflected by the return of most of the circulatory parameters to normal values, and furthermore, the sheep recovered from the effects of endotoxin. The exact mechanism by which anesthesia altered the response of the sheep to endotoxin was not clear. Depression of the baroreceptor and neurohumoral compensatory mechanisms was suggested. The present data indicated that dopamine was a useful agent in correcting the cardiovascular changes brought about by endotoxin."}
{"id": "PMID:472993", "title": "Hormonal aspects of suction drainage after thyroidectomy.", "content": "Suction drainage, after thyroidectomy, for a variety of conditions, varied between zero and 92.5 milliliters. The levels of thyroxine in this fluid were greater than those in the serum, at the same time being an average of 14.0 micrograms per 100 milliliters. The loss of thyroxine for 24 hours could represent up to 11.6 per cent of the total serum thyroxine pool. The serum thyroxine level, after initial fluctuation in either direction settled down, or up, to an average euthyroid level of 6.09 micrograms per 100 milliliters by the 21st day after operation. Possible factors influencing these changes have been considered and discussed. Such changes did not outweigh the advantages most surgeons found in drainage but could be eliminated if the ideal of no drainage could be attained. There was no evidence of exfoliated acinar cells in the drainage fluid.", "contents": "Hormonal aspects of suction drainage after thyroidectomy. Suction drainage, after thyroidectomy, for a variety of conditions, varied between zero and 92.5 milliliters. The levels of thyroxine in this fluid were greater than those in the serum, at the same time being an average of 14.0 micrograms per 100 milliliters. The loss of thyroxine for 24 hours could represent up to 11.6 per cent of the total serum thyroxine pool. The serum thyroxine level, after initial fluctuation in either direction settled down, or up, to an average euthyroid level of 6.09 micrograms per 100 milliliters by the 21st day after operation. Possible factors influencing these changes have been considered and discussed. Such changes did not outweigh the advantages most surgeons found in drainage but could be eliminated if the ideal of no drainage could be attained. There was no evidence of exfoliated acinar cells in the drainage fluid."}
{"id": "PMID:472995", "title": "Recurrent stenosis after carotid endarterectomy.", "content": "Thirteen of 1,250 patients required a second operation for recurrent stenosis following carotid endarterectomy performed at the Cleveland Clinic between 1958 and 1978. Two other patients underwent reoperation because of recurrent stenosis following primary operations at other institutions. Thirteen of the 15 patients experienced neurologic symptoms caused by recurent stenosis, while two patients remained asymptomatic. Atherosclerosis was responsible for recurrent stenosis in 12 patients and appeared to be related to hypercholesterolemia. Three of the patients had myointimal fibroplasia. Eleven of the 16 reoperations for recurrent stenosis of the carotid artery consisted of carotid endarterectomy with vein patch angioplasty. Three patients had carotid endarterectomy with closure of the primary arteriotomy. One patient with occlusion of the internal carotid artery underwent endarterectomy of the external carotid artery because of amaurosis fugax, and a saphenous vein interposition graft was used to replace a previous Dacron graft in one patient with anastomotic stenosis. One patient had a stroke during reoperation manifest as multiple retinal emboli. Fourteen patients have remained asymptomatic from one to 70 months following reoperation. One patient with occlusion of the contralateral internal carotid artery has experienced persistent vertebrobasilar symptoms.", "contents": "Recurrent stenosis after carotid endarterectomy. Thirteen of 1,250 patients required a second operation for recurrent stenosis following carotid endarterectomy performed at the Cleveland Clinic between 1958 and 1978. Two other patients underwent reoperation because of recurrent stenosis following primary operations at other institutions. Thirteen of the 15 patients experienced neurologic symptoms caused by recurent stenosis, while two patients remained asymptomatic. Atherosclerosis was responsible for recurrent stenosis in 12 patients and appeared to be related to hypercholesterolemia. Three of the patients had myointimal fibroplasia. Eleven of the 16 reoperations for recurrent stenosis of the carotid artery consisted of carotid endarterectomy with vein patch angioplasty. Three patients had carotid endarterectomy with closure of the primary arteriotomy. One patient with occlusion of the internal carotid artery underwent endarterectomy of the external carotid artery because of amaurosis fugax, and a saphenous vein interposition graft was used to replace a previous Dacron graft in one patient with anastomotic stenosis. One patient had a stroke during reoperation manifest as multiple retinal emboli. Fourteen patients have remained asymptomatic from one to 70 months following reoperation. One patient with occlusion of the contralateral internal carotid artery has experienced persistent vertebrobasilar symptoms."}
{"id": "PMID:472996", "title": "A clinical evaluation of cricothyroidotomy.", "content": "Cricothyroidotomies were performed upon 147 patients at the New York University Medical Center and Booth Memorial Center from March 1976 through February 1978. Cricothyroidotomy was demonstrated to be a rapid and technically simple and precise procedure. The incidence of complications was 8.6 per cent. Catastrophic complications occurred in two patients who had severe laryngeal stenosis. Cricothyroidotomy was performed following prolonged endotracheal intubation in these two patients who had airway obstruction immediately following endotracheal extubation. In both patients, there was a glottic and subglottic component to the laryngeal stenosis suggesting that endotracheal intubation as well cricothyroidotomy played a critical part in the development of laryngeal stenosis. In view of these observations, we believe that cricothyroidotomy is useful, particularly in emergency situations and in patients with median sternotomy incisions but is contraindicated in patients having endotracheal intubation of more than seven days' duration or in patients having airway obstruction develop following removal of an endotracheal tube except as a temporary lifesaving procedure.", "contents": "A clinical evaluation of cricothyroidotomy. Cricothyroidotomies were performed upon 147 patients at the New York University Medical Center and Booth Memorial Center from March 1976 through February 1978. Cricothyroidotomy was demonstrated to be a rapid and technically simple and precise procedure. The incidence of complications was 8.6 per cent. Catastrophic complications occurred in two patients who had severe laryngeal stenosis. Cricothyroidotomy was performed following prolonged endotracheal intubation in these two patients who had airway obstruction immediately following endotracheal extubation. In both patients, there was a glottic and subglottic component to the laryngeal stenosis suggesting that endotracheal intubation as well cricothyroidotomy played a critical part in the development of laryngeal stenosis. In view of these observations, we believe that cricothyroidotomy is useful, particularly in emergency situations and in patients with median sternotomy incisions but is contraindicated in patients having endotracheal intubation of more than seven days' duration or in patients having airway obstruction develop following removal of an endotracheal tube except as a temporary lifesaving procedure."}
{"id": "PMID:472998", "title": "Objective improvement after aortofemoral bypass for exercise ischemia.", "content": "The physiologic and functional improvement after aortofemoral bypass was quantitatively assessed by preoperative and postoperative noninvasive vascular testing in two groups of patients operated upon for claudication alone, those with significant occlusive disease of the lower extremity limited to the aortoiliac segments and those with combined disease of both the aortoiliac and superficial femoral arteries. While there is a statistically significant increase in segmental pressures and exercise times after aortofemoral bypass in the group with combined disease, these objective hemodynamic parameters are far from normal and are statistically significantly lower than values obtained in the group of patients with open superficial femoral arteries. Accordingly, we question the advisability of proximal arterial reconstruction in patients with combined hemodynamically significant aortoiliac and occlusive disease of the superficial femoral artery and claudication as the only indication for operation.", "contents": "Objective improvement after aortofemoral bypass for exercise ischemia. The physiologic and functional improvement after aortofemoral bypass was quantitatively assessed by preoperative and postoperative noninvasive vascular testing in two groups of patients operated upon for claudication alone, those with significant occlusive disease of the lower extremity limited to the aortoiliac segments and those with combined disease of both the aortoiliac and superficial femoral arteries. While there is a statistically significant increase in segmental pressures and exercise times after aortofemoral bypass in the group with combined disease, these objective hemodynamic parameters are far from normal and are statistically significantly lower than values obtained in the group of patients with open superficial femoral arteries. Accordingly, we question the advisability of proximal arterial reconstruction in patients with combined hemodynamically significant aortoiliac and occlusive disease of the superficial femoral artery and claudication as the only indication for operation."}
{"id": "PMID:472999", "title": "Effects of cooling and intraluminally administered antiseptics on surgically induced ischemia of the intestine in dogs.", "content": "The efficacy of surface cooling and intraluminal antiseptic solutions for short term preservation of intestinal segments in preparation for free tissue transfer were investigated. Five segments of the small intestine and five segments of the colon were allocated to each group as follows: group 1, ischemia at 37 degrees C.; group 2, ischemia at 6 to 12 degrees C., and group 3, ischemia at 6 to 12 degrees C. plus povidone-iodine intraluminally. Histologic grading of segments allowed assessment of the efficacy of short term protection against ischemic changes. Surface cooling effectively prevented histologic evidence of ischemia. No additional benefit was observed using povidone-iodine intraluminally. Significant changes in tissue water content and evidence of bacterial invasion were not observed.", "contents": "Effects of cooling and intraluminally administered antiseptics on surgically induced ischemia of the intestine in dogs. The efficacy of surface cooling and intraluminal antiseptic solutions for short term preservation of intestinal segments in preparation for free tissue transfer were investigated. Five segments of the small intestine and five segments of the colon were allocated to each group as follows: group 1, ischemia at 37 degrees C.; group 2, ischemia at 6 to 12 degrees C., and group 3, ischemia at 6 to 12 degrees C. plus povidone-iodine intraluminally. Histologic grading of segments allowed assessment of the efficacy of short term protection against ischemic changes. Surface cooling effectively prevented histologic evidence of ischemia. No additional benefit was observed using povidone-iodine intraluminally. Significant changes in tissue water content and evidence of bacterial invasion were not observed."}
{"id": "PMID:473000", "title": "The effect of intermittent compression of the calf on the fibrinolytic responses in the blood during a surgical operation.", "content": "The fibrinolytic responses in the blood during surgical operation have been studied in two groups of patients during intraoperative intermittent compression of the calf. Fibrinolytic activity did not differ significantly between the groups. The postoperative fibrinolytic shutdown was not prevented by intermittent compression of the calf. It is concluded that, whatever the mechanism by which venous thrombosis is prevented by intermittent compression of the calf, it is not by further stimulation of systemic fibrinolysis.", "contents": "The effect of intermittent compression of the calf on the fibrinolytic responses in the blood during a surgical operation. The fibrinolytic responses in the blood during surgical operation have been studied in two groups of patients during intraoperative intermittent compression of the calf. Fibrinolytic activity did not differ significantly between the groups. The postoperative fibrinolytic shutdown was not prevented by intermittent compression of the calf. It is concluded that, whatever the mechanism by which venous thrombosis is prevented by intermittent compression of the calf, it is not by further stimulation of systemic fibrinolysis."}
{"id": "PMID:473003", "title": "Arterial monitoring through the inferior epigastric artery during celiotomy.", "content": "We have performed inferior epigastric artery cannulation for arterial pressure monitoring and blood sampling in ten patients with no associated complications. This site is easily accessible upon celiotomy, expendable and secure. For these reasons, we believe cannulation of the inferior epigastric artery is the preferred method of arterial monitoring in the patient undergoing celiotomy.", "contents": "Arterial monitoring through the inferior epigastric artery during celiotomy. We have performed inferior epigastric artery cannulation for arterial pressure monitoring and blood sampling in ten patients with no associated complications. This site is easily accessible upon celiotomy, expendable and secure. For these reasons, we believe cannulation of the inferior epigastric artery is the preferred method of arterial monitoring in the patient undergoing celiotomy."}
{"id": "PMID:473006", "title": "Osteomyelitis of the skull.", "content": "This retrospective study includes 18 patients who underwent 28 admissions for treatment of osteomyelitis of the skull. Each admission was reviewed separately. Systemic symptoms were rare and signalled the presence of an associated collection of pus. Films of the skull, polytomography and bone scans were all useful in establishing the diagnosis, whereas white blood cell count, erythrocyte sedimentation rate and brain scans were of little value. Complete surgical debridement was found to be of significantly greater value than limited surgical debridement. The surgical results appeared to be improved when surgery was followed by long courses of antibiotics. Each patient who received complete surgical debridement followed by at least six weeks of antibiotic therapy was cured.", "contents": "Osteomyelitis of the skull. This retrospective study includes 18 patients who underwent 28 admissions for treatment of osteomyelitis of the skull. Each admission was reviewed separately. Systemic symptoms were rare and signalled the presence of an associated collection of pus. Films of the skull, polytomography and bone scans were all useful in establishing the diagnosis, whereas white blood cell count, erythrocyte sedimentation rate and brain scans were of little value. Complete surgical debridement was found to be of significantly greater value than limited surgical debridement. The surgical results appeared to be improved when surgery was followed by long courses of antibiotics. Each patient who received complete surgical debridement followed by at least six weeks of antibiotic therapy was cured."}
{"id": "PMID:473007", "title": "Intracranial germinomas: treatment and results.", "content": "Eighty-nine patients with intracranial germinomas were treated from 1954 through 1975. Radiotherapy was given in 62 patients. The five-year survival rate was 61% and the ten-year survival rate was 55%. In unbiopsied patients the five-year survival rate was 66% and the ten-year survival rate was 59%, whereas in biopsied ones it was 56% and 47%, respectively. Prophylactic irradiation to the spinal axis in the presence of germinoma cells in the cerebrospinal fluid may not be justified.", "contents": "Intracranial germinomas: treatment and results. Eighty-nine patients with intracranial germinomas were treated from 1954 through 1975. Radiotherapy was given in 62 patients. The five-year survival rate was 61% and the ten-year survival rate was 55%. In unbiopsied patients the five-year survival rate was 66% and the ten-year survival rate was 59%, whereas in biopsied ones it was 56% and 47%, respectively. Prophylactic irradiation to the spinal axis in the presence of germinoma cells in the cerebrospinal fluid may not be justified."}
{"id": "PMID:473008", "title": "Vermian hematoma in a four-year-old child.", "content": "An unusual case of a vermian hematoma from the rupture of an angioma in a four-year-old child is presented. The lesion was treated successfully with complete recovery.", "contents": "Vermian hematoma in a four-year-old child. An unusual case of a vermian hematoma from the rupture of an angioma in a four-year-old child is presented. The lesion was treated successfully with complete recovery."}
{"id": "PMID:473009", "title": "Interhemispheric subdural empyema with enlarged meningeal arteries.", "content": "A successfully treated case of an interhemispheric subdural empyema is reported. In addition to the characteristic angiographic and computed tomographic findings, the present case was accompanied by enlarged middle meningeal and anterior falx arteries.", "contents": "Interhemispheric subdural empyema with enlarged meningeal arteries. A successfully treated case of an interhemispheric subdural empyema is reported. In addition to the characteristic angiographic and computed tomographic findings, the present case was accompanied by enlarged middle meningeal and anterior falx arteries."}
{"id": "PMID:473010", "title": "A comparative study of performance characteristics of cerebral aneurysm clips.", "content": "The authors have developed a procedure to evaluate the performance of cerebral aneurysm clips as a function of intravascular pressure and aneurysm neck diameter. Numerous models of Mayfield, Scoville, Variangle, Heifetz and Yasargil clips were tested. The various clips demonstrated decreasing capabilities for secure occlusion of a vessel as  vascular diameters and intravascular pressure increased. These findings are in record with predictions based upon La Place's Principle and indicate that the size of an aneurysm must be considered in the choice of an appropriate aneurysm clip.", "contents": "A comparative study of performance characteristics of cerebral aneurysm clips. The authors have developed a procedure to evaluate the performance of cerebral aneurysm clips as a function of intravascular pressure and aneurysm neck diameter. Numerous models of Mayfield, Scoville, Variangle, Heifetz and Yasargil clips were tested. The various clips demonstrated decreasing capabilities for secure occlusion of a vessel as  vascular diameters and intravascular pressure increased. These findings are in record with predictions based upon La Place's Principle and indicate that the size of an aneurysm must be considered in the choice of an appropriate aneurysm clip."}
{"id": "PMID:473011", "title": "Brain abscess after transnasal intracranial penetration of a paint-brush.", "content": "The case of a brain abscess caused by intracranial, transnasal penetration of a paint-brush is presented. The danger that such foreign bodies may remain unnoticed, especially in children, is stressed. Cure was achieved by total resection of the abscess capsule.", "contents": "Brain abscess after transnasal intracranial penetration of a paint-brush. The case of a brain abscess caused by intracranial, transnasal penetration of a paint-brush is presented. The danger that such foreign bodies may remain unnoticed, especially in children, is stressed. Cure was achieved by total resection of the abscess capsule."}
{"id": "PMID:473012", "title": "Traumatic cerebral swelling developing within 30 minutes after injury.", "content": "Development of acute massive cerebral swelling within 30 minutes after closed head injury is documented by computed tomography. The swelling was rapidly resolved by steroid treatment. Detection and treatment of acute traumatic cerebral swelling are discussed briefly.", "contents": "Traumatic cerebral swelling developing within 30 minutes after injury. Development of acute massive cerebral swelling within 30 minutes after closed head injury is documented by computed tomography. The swelling was rapidly resolved by steroid treatment. Detection and treatment of acute traumatic cerebral swelling are discussed briefly."}
{"id": "PMID:473013", "title": "Intracerebral ciliated epithelial cyst.", "content": "The authors describe a case of hemispheric brain cyst of possible neuroectodermal origin, presenting as a frontotemporoparietal mass lesion. The etiologic aspects of intracranial neuroepithelial cysts are discussed.", "contents": "Intracerebral ciliated epithelial cyst. The authors describe a case of hemispheric brain cyst of possible neuroectodermal origin, presenting as a frontotemporoparietal mass lesion. The etiologic aspects of intracranial neuroepithelial cysts are discussed."}
{"id": "PMID:473014", "title": "Spontaneous spinal subdural haematoma during general anaesthesia.", "content": "Spontaneous spinal subdural haematoma causing acute spinal cord compression is a well recognized condition and often responds well to early surgical intervention. In the elderly, the haematoma usually occurs as a result of minor trauma or atherosclerosis with hypertension. We present in this report the history of a patient who became paraplegic during a general anaesthetic for an operation for removal of a ureteral calculus. Rupture of a spinal vascular malformation was found to be responsible for the subdural haematoma, which remained undiagnosed until surgical exploration.", "contents": "Spontaneous spinal subdural haematoma during general anaesthesia. Spontaneous spinal subdural haematoma causing acute spinal cord compression is a well recognized condition and often responds well to early surgical intervention. In the elderly, the haematoma usually occurs as a result of minor trauma or atherosclerosis with hypertension. We present in this report the history of a patient who became paraplegic during a general anaesthetic for an operation for removal of a ureteral calculus. Rupture of a spinal vascular malformation was found to be responsible for the subdural haematoma, which remained undiagnosed until surgical exploration."}
{"id": "PMID:473016", "title": "Peripheral enhancement in computerized cranial tomography: a non-specific finding.", "content": "Peripheral enhancement of intracranial lesions on CT examination is still being considered by some radiologists as specific for an abscess or a cystic tumor. A review of the last 4,000 scans at this institution has demonstrated that it is neither specific nor, in many cases, of much help in the differential diagnosis of the lesions. Examples of the various types of lesions that can demonstrate peripheral enhancement are shown. It is suggested that many if not all types of pathology would demonstrate this effect if the cranial CT scans were done at a fortuitous time or sequentially. Some differential diagnostic suggestions are also made.", "contents": "Peripheral enhancement in computerized cranial tomography: a non-specific finding. Peripheral enhancement of intracranial lesions on CT examination is still being considered by some radiologists as specific for an abscess or a cystic tumor. A review of the last 4,000 scans at this institution has demonstrated that it is neither specific nor, in many cases, of much help in the differential diagnosis of the lesions. Examples of the various types of lesions that can demonstrate peripheral enhancement are shown. It is suggested that many if not all types of pathology would demonstrate this effect if the cranial CT scans were done at a fortuitous time or sequentially. Some differential diagnostic suggestions are also made."}
{"id": "PMID:473017", "title": "Rim enhancement in computed tomography with chronic epidural hematoma.", "content": "Three cases of epidural hematoma with delayed clinical course are presented. In each patient, computed tomography (CT) initially failed to show typical biconvex, high density extra-axial lesion. The correct diagnosis was reached only after injection of contrast medium revealed enhancement of the border of the hematoma. Importance of contrast enhancement in selected patients with head injury is stress, and the mechanism of rim enhancement is discussed.", "contents": "Rim enhancement in computed tomography with chronic epidural hematoma. Three cases of epidural hematoma with delayed clinical course are presented. In each patient, computed tomography (CT) initially failed to show typical biconvex, high density extra-axial lesion. The correct diagnosis was reached only after injection of contrast medium revealed enhancement of the border of the hematoma. Importance of contrast enhancement in selected patients with head injury is stress, and the mechanism of rim enhancement is discussed."}
{"id": "PMID:473018", "title": "Venous malformation of the pineal region.", "content": "A single and perhaps unique case of a venous malformation over the quadrigeminal plate causing acute obstructive hydrocephalus is reported. The lesion was well demonstrated by means of CAT scan but cerebral angiography failed to reveal the vascular nature of this lesion. Complete thrombosis of the lesion was achieved by surgical intervention and the resolution of the lesion was demonstrated by CAT scan. The patient remains asymptomatic and neurologically intact six months after the operation.", "contents": "Venous malformation of the pineal region. A single and perhaps unique case of a venous malformation over the quadrigeminal plate causing acute obstructive hydrocephalus is reported. The lesion was well demonstrated by means of CAT scan but cerebral angiography failed to reveal the vascular nature of this lesion. Complete thrombosis of the lesion was achieved by surgical intervention and the resolution of the lesion was demonstrated by CAT scan. The patient remains asymptomatic and neurologically intact six months after the operation."}
{"id": "PMID:473019", "title": "Primary sellar chondromas.", "content": "Two cases of chondromas arising primarily from the sella turcica are reported. The eight cases reported in the literature are reviewed. The basic clinical and radiological findings of these tumors and the difficulties of diagnosis and treatment are discussed.", "contents": "Primary sellar chondromas. Two cases of chondromas arising primarily from the sella turcica are reported. The eight cases reported in the literature are reviewed. The basic clinical and radiological findings of these tumors and the difficulties of diagnosis and treatment are discussed."}
{"id": "PMID:473020", "title": "Anticoagulants and intracerebral hemorrhage from an unsuspected meningioma.", "content": "A unique case of a patient with an undiagnosed convexity meningioma is presented. Hemorrhage into the tumor, following the administration of anticoagulant drugs for the treatment of thrombophlebitis, resulted in severe neurological defects and the eventual demise of the patient.", "contents": "Anticoagulants and intracerebral hemorrhage from an unsuspected meningioma. A unique case of a patient with an undiagnosed convexity meningioma is presented. Hemorrhage into the tumor, following the administration of anticoagulant drugs for the treatment of thrombophlebitis, resulted in severe neurological defects and the eventual demise of the patient."}
{"id": "PMID:473022", "title": "Combination chemotherapy with VM 26 and CCNU in primary malignant brain tumors.", "content": "Twenty-seven patients with inoperable or recurrent primary malignant brain tumors after previous resection and/or radiotherapy, were treated with corticosteroids and a combination chemotherapy consisting of VM 26 and CCNU. There were 15 (55%) patients that responded, three with a complete and 12 with a partial response with a mean duration of response of 13.4 months. Toxicity was mainly hematological and of acceptable degree. Combination chemotherapy with VM 26 and CCNU is well tolerated and can be administered on an out-patient basis. In our experience it is better than single agent chemotherapy.", "contents": "Combination chemotherapy with VM 26 and CCNU in primary malignant brain tumors. Twenty-seven patients with inoperable or recurrent primary malignant brain tumors after previous resection and/or radiotherapy, were treated with corticosteroids and a combination chemotherapy consisting of VM 26 and CCNU. There were 15 (55%) patients that responded, three with a complete and 12 with a partial response with a mean duration of response of 13.4 months. Toxicity was mainly hematological and of acceptable degree. Combination chemotherapy with VM 26 and CCNU is well tolerated and can be administered on an out-patient basis. In our experience it is better than single agent chemotherapy."}
{"id": "PMID:473023", "title": "Experimentally induced cerebral aneurysms in rats: part II.", "content": "An experimental condition that permits easy inducement of cerebral aneurysms in rats is reported. Previously, we demonstrated that saccular cerebral aneurysms were inducible in experimental animals and suggested that adult rats might be more preferable for inducing the lesion. Therefore, a longer-term study was performed intending to increase the incidence of cerebral aneurysms, using only adult rats. Adult rats were treated with ligation of one common carotid arter, deoxycorticosterone and salt hypertension, and beta-aminopropionitrile. Cerebral aneurysms developed in eleven of 30 rats which died 11-21 weeks after the start of the beta-aminopropionitrile feeding. All aneurysms were located on the anterior cerebral-anterior communicating arterial complex. Contributory mechanisms are discussed.", "contents": "Experimentally induced cerebral aneurysms in rats: part II. An experimental condition that permits easy inducement of cerebral aneurysms in rats is reported. Previously, we demonstrated that saccular cerebral aneurysms were inducible in experimental animals and suggested that adult rats might be more preferable for inducing the lesion. Therefore, a longer-term study was performed intending to increase the incidence of cerebral aneurysms, using only adult rats. Adult rats were treated with ligation of one common carotid arter, deoxycorticosterone and salt hypertension, and beta-aminopropionitrile. Cerebral aneurysms developed in eleven of 30 rats which died 11-21 weeks after the start of the beta-aminopropionitrile feeding. All aneurysms were located on the anterior cerebral-anterior communicating arterial complex. Contributory mechanisms are discussed."}
{"id": "PMID:473025", "title": "Primary aortoduodenal fistula: extra-anatomic vascular reconstruction not required for successful management.", "content": "Eleven patients reported in the literature and three patients presented herein have survived treatment of primary aortoduodenal fistula by standard aortic reconstruction with prosthesis placement and duodenal repair. Follow-up of these patients documents that 93% (13 of 14) have had no graft-related complications. Standard aortic reconstruction appears to be justified in the treatment of primary aortoduodenal fistula.", "contents": "Primary aortoduodenal fistula: extra-anatomic vascular reconstruction not required for successful management. Eleven patients reported in the literature and three patients presented herein have survived treatment of primary aortoduodenal fistula by standard aortic reconstruction with prosthesis placement and duodenal repair. Follow-up of these patients documents that 93% (13 of 14) have had no graft-related complications. Standard aortic reconstruction appears to be justified in the treatment of primary aortoduodenal fistula."}
{"id": "PMID:473028", "title": "The sepsis-glucose intolerance riddle: a hormonal explanation.", "content": "Glucose intolerance has been commonly observed in sepsis and has been attributed to a multitude of endocrine and metabolic disorders. From 1977 to 1978, 19 patients were studied using intravenous glucose tolerance tests to evaluate this phenomenon; 15 patients presented with ongoing sepsis and four patients served as stress controls. Glucose intolerance was found to be a significant finding in less than 40% of the septic group. This state of intolerance was noted to be associated with a high mortality rate (60%), whereas glucose tolerance in sepsis was associated with a much improved mortality rate (10%). Hormone levels were correlated with glucose tolerance curves using the parameters of insulin, glucagon, growth hormone, cortisol, and epinephrine levels. Glucose intolerance and a high mortality rate were linked to sustained hyperglucagonemia, which was unresponsive to glucose challenge, and to marked suppression of growth hormone. This apparently represents a decompensated peripheral metabolic energy deficit, which results in the increased mortality rate.", "contents": "The sepsis-glucose intolerance riddle: a hormonal explanation. Glucose intolerance has been commonly observed in sepsis and has been attributed to a multitude of endocrine and metabolic disorders. From 1977 to 1978, 19 patients were studied using intravenous glucose tolerance tests to evaluate this phenomenon; 15 patients presented with ongoing sepsis and four patients served as stress controls. Glucose intolerance was found to be a significant finding in less than 40% of the septic group. This state of intolerance was noted to be associated with a high mortality rate (60%), whereas glucose tolerance in sepsis was associated with a much improved mortality rate (10%). Hormone levels were correlated with glucose tolerance curves using the parameters of insulin, glucagon, growth hormone, cortisol, and epinephrine levels. Glucose intolerance and a high mortality rate were linked to sustained hyperglucagonemia, which was unresponsive to glucose challenge, and to marked suppression of growth hormone. This apparently represents a decompensated peripheral metabolic energy deficit, which results in the increased mortality rate."}
{"id": "PMID:473029", "title": "The use of photoplethysmography in the assessment of venous insufficiency: a comparison to venous pressure measurements.", "content": "In an attempt to develop a noninvasive test to assess objectively the magnitude of venous valvular dysfunction in pathologic states, photoplethysmography (PPG) was compared to venous pressure in the saphenous vein at the ankle. Simultaneous venous pressure and PPG recordings were taken before, during, and after exercise with subjects in the sitting position. In a total of 338 paired measurements in 24 normal, 25 postphlebitic, and 14 varicose limbs, PPG and venous pressure tracings appeared to be identical; data points had a correlation coefficient of great significance (r = 0.898). Postexercise recovery times clearly separated the normal from the postphlebitic limbs. Varicose limbs were assessed accurately regarding results of proposed surgery using an above-knee tourniquet. It appears that the PPG evaluation provides information comparable to venous pressure studies and does so more quickly and noninvasively. The test holds promise in measuring results of direct venous reconstructive surgery as well as in venectomy procedures.", "contents": "The use of photoplethysmography in the assessment of venous insufficiency: a comparison to venous pressure measurements. In an attempt to develop a noninvasive test to assess objectively the magnitude of venous valvular dysfunction in pathologic states, photoplethysmography (PPG) was compared to venous pressure in the saphenous vein at the ankle. Simultaneous venous pressure and PPG recordings were taken before, during, and after exercise with subjects in the sitting position. In a total of 338 paired measurements in 24 normal, 25 postphlebitic, and 14 varicose limbs, PPG and venous pressure tracings appeared to be identical; data points had a correlation coefficient of great significance (r = 0.898). Postexercise recovery times clearly separated the normal from the postphlebitic limbs. Varicose limbs were assessed accurately regarding results of proposed surgery using an above-knee tourniquet. It appears that the PPG evaluation provides information comparable to venous pressure studies and does so more quickly and noninvasively. The test holds promise in measuring results of direct venous reconstructive surgery as well as in venectomy procedures."}
{"id": "PMID:473030", "title": "Hemodynamic studies before and after extended bypass grafts to the tibial and personeal arteries.", "content": "Thirty-ones limbs of 29 consecutive patients undergoing bypass grafts to the tibial or peroneal arteries were studied. Twenty-five limbs had ischemic rest pain with or without localized gangrene, and six had incapacitating claudication. The mean segmental pressure indices were upper thigh, 1.16 +/- 0.36; above knee, 0.71 +/- 0.30; below knee 0.51 +/- 0.23; and ankle, 0.28 +/- 0.21. Indices at the ankle were significantly lower (P less than 0.005) than those obtained from limbs that were candidates for simple femoropopliteal bypass. Three grafts failed immediately (initial patency rate of 90%). In the surviving grafts, ankle pressures rose from 39 +/- 26 to 145 +/- 29 mm Hg, and the ankle pressure index increased from 0.27 +/- 0.20 to 1.03 +/- 0.15 mm Hg. Consistent with these hemodynamic changes, all patients but one initially were relieved of their symptoms, and all ischemic lesions were treated successfully. Thirteen grafts failed subsequently (21 days to 34 months). Compared with preoperative values, ankle pressures after failure were either increased or unchanged in 75% of these limbs. Early occlusion could not be predicted on the basis of a low preoperative pressure index. During follow-up, impending graft failure was detected by a fall in ankle pressure in three patients and successfully prevented.", "contents": "Hemodynamic studies before and after extended bypass grafts to the tibial and personeal arteries. Thirty-ones limbs of 29 consecutive patients undergoing bypass grafts to the tibial or peroneal arteries were studied. Twenty-five limbs had ischemic rest pain with or without localized gangrene, and six had incapacitating claudication. The mean segmental pressure indices were upper thigh, 1.16 +/- 0.36; above knee, 0.71 +/- 0.30; below knee 0.51 +/- 0.23; and ankle, 0.28 +/- 0.21. Indices at the ankle were significantly lower (P less than 0.005) than those obtained from limbs that were candidates for simple femoropopliteal bypass. Three grafts failed immediately (initial patency rate of 90%). In the surviving grafts, ankle pressures rose from 39 +/- 26 to 145 +/- 29 mm Hg, and the ankle pressure index increased from 0.27 +/- 0.20 to 1.03 +/- 0.15 mm Hg. Consistent with these hemodynamic changes, all patients but one initially were relieved of their symptoms, and all ischemic lesions were treated successfully. Thirteen grafts failed subsequently (21 days to 34 months). Compared with preoperative values, ankle pressures after failure were either increased or unchanged in 75% of these limbs. Early occlusion could not be predicted on the basis of a low preoperative pressure index. During follow-up, impending graft failure was detected by a fall in ankle pressure in three patients and successfully prevented."}
{"id": "PMID:473032", "title": "Results of subtotal parathyroidectomy for primary chief cell hyperplasia.", "content": "Analysis of the long-term results of subtotal parathyroidectomy in patients with primary chief cell hyperplasia provides evidence that refutes the recent assertion that such treatment is often ineffective. With the use of rigid criteria, 55 patients with unequivocal chief cell hyperplasia were culled from 1,576 patients who had been operated on for primary hyperparathyroidism at the Mayo Clinic between July, 1959, and July, 1976. Follow-up information, including serum levels of calcium, was obtained for all patients up to at least July, 1977. Follow-up for surviving patients ranged from 1 year to 17 years (average, 3.9 years). Seven patients (13%) were not cured by subtotal parathyroidectomy and were left with persistent hyperparathyroidism after operation, presumably because a supernumerary gland was overlooked. However, not a single patient experienced recurrent hyperparathyroidism during the 213 patient-years of follow-up. In only three patients (5%) did permanent hypoparathyroidism develop following operation. These results reaffirm the efficacy of subtotal parathyroidectomy for primary chief cell hyperplasia and call into serious question the recent advocacy of total parathyroidectomy and autotransplantation of parathyroid tissue in these cases.", "contents": "Results of subtotal parathyroidectomy for primary chief cell hyperplasia. Analysis of the long-term results of subtotal parathyroidectomy in patients with primary chief cell hyperplasia provides evidence that refutes the recent assertion that such treatment is often ineffective. With the use of rigid criteria, 55 patients with unequivocal chief cell hyperplasia were culled from 1,576 patients who had been operated on for primary hyperparathyroidism at the Mayo Clinic between July, 1959, and July, 1976. Follow-up information, including serum levels of calcium, was obtained for all patients up to at least July, 1977. Follow-up for surviving patients ranged from 1 year to 17 years (average, 3.9 years). Seven patients (13%) were not cured by subtotal parathyroidectomy and were left with persistent hyperparathyroidism after operation, presumably because a supernumerary gland was overlooked. However, not a single patient experienced recurrent hyperparathyroidism during the 213 patient-years of follow-up. In only three patients (5%) did permanent hypoparathyroidism develop following operation. These results reaffirm the efficacy of subtotal parathyroidectomy for primary chief cell hyperplasia and call into serious question the recent advocacy of total parathyroidectomy and autotransplantation of parathyroid tissue in these cases."}
{"id": "PMID:473033", "title": "Primary aldosteronism: experience with thirty-eight patients.", "content": "Thirty-eight hypertensive, hypokalemic patients underwent adrenalectomy for primary aldosteronism. Thirty-one patients were found to have an adenoma and seven patients \"idiopathic\" hyperplasia. The diagnosis was made by finding low plasma renin activity, which could not be stimulated, and unsuppressable elevated plasma or urine aldosterone. The distinction between adenoma and hyperplasia and the localization of an adenoma were accomplished by adrenal venography, adrenal vein blood analysis, and iodocholesterol scanning. Venography was accurate in 87%; adrenal vein blood analysis in 91%; and iodocholesterol scanning in 72%. Dexamethazone suppressed scanning heightened discrimination to 91%. The adenomas were equally distributed between the right and left adrenal gland, with one patient having bilateral adenomas. All but two patients underwent adrenalectomy from a posterior lumbar incision. Postoperative recovery was uncomplicated. Eighteen months after operation 77% of patients with an adenoma were normotensive.", "contents": "Primary aldosteronism: experience with thirty-eight patients. Thirty-eight hypertensive, hypokalemic patients underwent adrenalectomy for primary aldosteronism. Thirty-one patients were found to have an adenoma and seven patients \"idiopathic\" hyperplasia. The diagnosis was made by finding low plasma renin activity, which could not be stimulated, and unsuppressable elevated plasma or urine aldosterone. The distinction between adenoma and hyperplasia and the localization of an adenoma were accomplished by adrenal venography, adrenal vein blood analysis, and iodocholesterol scanning. Venography was accurate in 87%; adrenal vein blood analysis in 91%; and iodocholesterol scanning in 72%. Dexamethazone suppressed scanning heightened discrimination to 91%. The adenomas were equally distributed between the right and left adrenal gland, with one patient having bilateral adenomas. All but two patients underwent adrenalectomy from a posterior lumbar incision. Postoperative recovery was uncomplicated. Eighteen months after operation 77% of patients with an adenoma were normotensive."}
{"id": "PMID:473034", "title": "Thoraco-omphalopagus conjoined twins: successful surgical separation.", "content": "Male thoraco-omphalopagus conjoined twins were evaluated systematically with respect to visceral joining. Cardiac studies included electrocardiography, echocardiography, and a dynamic radioactive heart scan. No union was found. Liver union was demonstrated by scanning and angiographic studies. No communication between the gastrointestinal tracts was found, and no other major anomalies were discovered. When the twins were approximately 3 months of age, surgical separation was performed. A common pericardium, two separate hearts, and a union of the liver were found. Prosthetic sheeting was used to close the pericardium and the thoracic-abdominal defects in both. In one, primary skin closure was possible. In the other silicone rubber sheeting was used to close a large skin defect in the chest. Both have done well over a follow-up period of 14 months. One is larger than the other, but the growth rate of both has improved in the last 6 months.", "contents": "Thoraco-omphalopagus conjoined twins: successful surgical separation. Male thoraco-omphalopagus conjoined twins were evaluated systematically with respect to visceral joining. Cardiac studies included electrocardiography, echocardiography, and a dynamic radioactive heart scan. No union was found. Liver union was demonstrated by scanning and angiographic studies. No communication between the gastrointestinal tracts was found, and no other major anomalies were discovered. When the twins were approximately 3 months of age, surgical separation was performed. A common pericardium, two separate hearts, and a union of the liver were found. Prosthetic sheeting was used to close the pericardium and the thoracic-abdominal defects in both. In one, primary skin closure was possible. In the other silicone rubber sheeting was used to close a large skin defect in the chest. Both have done well over a follow-up period of 14 months. One is larger than the other, but the growth rate of both has improved in the last 6 months."}
{"id": "PMID:473035", "title": "Triple endoscopy: a neglected essential in head and neck cancer.", "content": "Surgeons are sometimes embarrassed to discover a second unanticipated cancer while focusing attention on an already evident cancer in the head and neck area, the bronchus, or the esophagus. we began in October, 1976, to do direct laryngoscopy, esophagoscopy, and bronchoscopy on each of the 134 cases admitted to our unit with suspected head and neck cancer. In 10 of the 134 patients in whom triple endoscopy was done, no malignancy was found. Of 24 of 124 (20%) patients with head and neck cancer, 16 patients (13%) had synchronous carcinoma and eight patients (7%) had metachronous carcinoma in this 2-year period. The incidence can be expected to rise steadily with time and mandates \"triple endoscopy\" on all patients with a suspected cancer in the head and neck area, the bronchus, or the esophagus, as these structures form a closely related unstable field. The discovery of several patients with dysplastic mucosal changes, carcinoma in situ, or very early invasive cancer, not only of the head and neck but also of the esophagus and bronchus, presents new problems of management.", "contents": "Triple endoscopy: a neglected essential in head and neck cancer. Surgeons are sometimes embarrassed to discover a second unanticipated cancer while focusing attention on an already evident cancer in the head and neck area, the bronchus, or the esophagus. we began in October, 1976, to do direct laryngoscopy, esophagoscopy, and bronchoscopy on each of the 134 cases admitted to our unit with suspected head and neck cancer. In 10 of the 134 patients in whom triple endoscopy was done, no malignancy was found. Of 24 of 124 (20%) patients with head and neck cancer, 16 patients (13%) had synchronous carcinoma and eight patients (7%) had metachronous carcinoma in this 2-year period. The incidence can be expected to rise steadily with time and mandates \"triple endoscopy\" on all patients with a suspected cancer in the head and neck area, the bronchus, or the esophagus, as these structures form a closely related unstable field. The discovery of several patients with dysplastic mucosal changes, carcinoma in situ, or very early invasive cancer, not only of the head and neck but also of the esophagus and bronchus, presents new problems of management."}
{"id": "PMID:473066", "title": "Teratogenic effects of nickel chloride on embryonic mice and its transfer to embryonic mice.", "content": "Administration of nickel chloride to the pregnant mice on the seventh to eleventh day of their gestational period, resulted in significant embryotoxic effects in terms of an increased resorption rate, a decreased fetal weight, delay in skeletal ossification and high incidence of malformation. Among the cases of fetal malformation, the following malformations were observed to occur at a higher rate of incidence: acephalia, exencephaly, cerebral hernia, open eyelid, cleft palate, micromelia, ankylosis of the extremity, club foot and skeletal anomalies. Most skeletal anomalies were in the form of vertebral and/or rib fusions and were found mostly at thoracic and lumbar levels. The concentration of nickel retained in embryonic tissues was 800 times higher in the exposed compared to control groups and indicated that increased tissue levels of nickel chloride had a toxic influence on the developing embryo.", "contents": "Teratogenic effects of nickel chloride on embryonic mice and its transfer to embryonic mice. Administration of nickel chloride to the pregnant mice on the seventh to eleventh day of their gestational period, resulted in significant embryotoxic effects in terms of an increased resorption rate, a decreased fetal weight, delay in skeletal ossification and high incidence of malformation. Among the cases of fetal malformation, the following malformations were observed to occur at a higher rate of incidence: acephalia, exencephaly, cerebral hernia, open eyelid, cleft palate, micromelia, ankylosis of the extremity, club foot and skeletal anomalies. Most skeletal anomalies were in the form of vertebral and/or rib fusions and were found mostly at thoracic and lumbar levels. The concentration of nickel retained in embryonic tissues was 800 times higher in the exposed compared to control groups and indicated that increased tissue levels of nickel chloride had a toxic influence on the developing embryo."}
{"id": "PMID:473067", "title": "Embryotoxicity of transplacentally and intraamniotically administered 6-azauridine in mice.", "content": "Embryotoxic effects were compared of intramuscularly (im) and intraamniotically (ia) administered 6-azauridine (Riboazauracil Spofa) in random-bred mice H-Velaz. Effects of single doses (0.25 mg, 2.5 mg, 25.0 mg and 250.0 mg for im and 0.0025 mg, 0.025 mg and 2.5 mg for ia administration) on days 11, 12, 13 and 14 were evaluated as a sum of dead fetuses and fetuses with cleft lip and/or palate, fetuses with limb deformities and fetuses with deformities constituting the syndrome of caudal regression (hypoplasia of the caudal part of the trunk, absent tail, short tail, curled tail). Considering the sensitivity peaks of the morphogenetic processes which were observed, the dose-response relationships, the transformation of the teratogenic to a lethal effect and critical period extension with increasing doses, it was found that the effects of ia and im administered 6-azauridine did not differ. It was concluded that ia administered 6-azauridine had direct effect on embryonic morphogenetic processes and that this, too, was the essential mechanism of embryotoxicity of im administered 6-azauridine. The value of the intraamniotic technique for establishing the direct embryotoxic effect is discussed.", "contents": "Embryotoxicity of transplacentally and intraamniotically administered 6-azauridine in mice. Embryotoxic effects were compared of intramuscularly (im) and intraamniotically (ia) administered 6-azauridine (Riboazauracil Spofa) in random-bred mice H-Velaz. Effects of single doses (0.25 mg, 2.5 mg, 25.0 mg and 250.0 mg for im and 0.0025 mg, 0.025 mg and 2.5 mg for ia administration) on days 11, 12, 13 and 14 were evaluated as a sum of dead fetuses and fetuses with cleft lip and/or palate, fetuses with limb deformities and fetuses with deformities constituting the syndrome of caudal regression (hypoplasia of the caudal part of the trunk, absent tail, short tail, curled tail). Considering the sensitivity peaks of the morphogenetic processes which were observed, the dose-response relationships, the transformation of the teratogenic to a lethal effect and critical period extension with increasing doses, it was found that the effects of ia and im administered 6-azauridine did not differ. It was concluded that ia administered 6-azauridine had direct effect on embryonic morphogenetic processes and that this, too, was the essential mechanism of embryotoxicity of im administered 6-azauridine. The value of the intraamniotic technique for establishing the direct embryotoxic effect is discussed."}
{"id": "PMID:473069", "title": "An aberrant nucleus in the telencephalon following administration of ENU during neuroembryogenesis.", "content": "Administration of ENU to rats during embryonic development caused the emergence of aberrant nuclei in the dorsal telencephalon. They were located in the corpus callosum, and were composed of pyramidal and stellate neurons. This suggested that the aberrant nuclei arose out of the neurons of cerebral cortex that had failed to migrate during embryogenesis. The aberrant nuclei were found predominantly in the animals receiving ENU on day 18 or earlier of gestation.", "contents": "An aberrant nucleus in the telencephalon following administration of ENU during neuroembryogenesis. Administration of ENU to rats during embryonic development caused the emergence of aberrant nuclei in the dorsal telencephalon. They were located in the corpus callosum, and were composed of pyramidal and stellate neurons. This suggested that the aberrant nuclei arose out of the neurons of cerebral cortex that had failed to migrate during embryogenesis. The aberrant nuclei were found predominantly in the animals receiving ENU on day 18 or earlier of gestation."}
{"id": "PMID:473070", "title": "The fetotoxic potential of municipal drinking water in the mouse.", "content": "Mice (CD-1 strain) were placed on diets containing either municipal drinking water (Durham, North Carolina) or water that had been distilled and passed through cartridges to reduce organics and remove inorganics. After a two-week acclimation period, animals were bred and pregnancy confirmed by the presence of a sperm plug. During the 8-month course of the study, approximately 500 pregnant mice were sacrificed on day 18 of gestation and their fetuses examined for visceral and skeletal anomalies. No significant water-related effects were found on any fetal parameter studied except for a 28.1% incidence of supernumerary ribs in the tap-water group as compared to 21.1% in the purified-water group. No differences were noted in the type or occurrence of anomalies between the two groups. A month to month variation was observed in a number of parameters. The degree of variation was similar for the treatment groups, suggesting that these changes might be random fluctuations.", "contents": "The fetotoxic potential of municipal drinking water in the mouse. Mice (CD-1 strain) were placed on diets containing either municipal drinking water (Durham, North Carolina) or water that had been distilled and passed through cartridges to reduce organics and remove inorganics. After a two-week acclimation period, animals were bred and pregnancy confirmed by the presence of a sperm plug. During the 8-month course of the study, approximately 500 pregnant mice were sacrificed on day 18 of gestation and their fetuses examined for visceral and skeletal anomalies. No significant water-related effects were found on any fetal parameter studied except for a 28.1% incidence of supernumerary ribs in the tap-water group as compared to 21.1% in the purified-water group. No differences were noted in the type or occurrence of anomalies between the two groups. A month to month variation was observed in a number of parameters. The degree of variation was similar for the treatment groups, suggesting that these changes might be random fluctuations."}
{"id": "PMID:473073", "title": "Cadmium induced limb defects in mice: strain associated differences in sensitivity.", "content": "Cadmium (CdSO4) was given ip on day 9 at 12 or 24 mumol/kg to pregnant CD-1 (non-inbred) mice. Fetuses showed malformations of the limbs, face, trunk, and tail. There was a statistically significant relationship between the dose of cadmium and the malformation rate. Cadmium (12 mumol/kg ip on day 9) was then given to mice of six inbred strains three of which (A/J, BALB/cJ, and C57BL6J) carry a gene cdm for resistance to cadmium-induced testicular damage, and three strains (AKR/J, CBA/J, and DBA/2J) which do not. Paradoxically, the three strains resistant to cadmium induced testicular damage were significantly more sensitive to its teratogenic effects than were the other three strains. In all inbred strains most malformations involved the limbs. All forelimb defects found in inbred or non-inbred cadmium treated mice were postaxial and indistinguishable from those produced by acetazolamide in mice. The remarkable similarity of the cadmium- and acetazolamide-induced forelimb malformations may be a reflection of the limited number of ways that a rodent forelimb can react to a teratogenic insult. The hindlimb defects were all preaxial.", "contents": "Cadmium induced limb defects in mice: strain associated differences in sensitivity. Cadmium (CdSO4) was given ip on day 9 at 12 or 24 mumol/kg to pregnant CD-1 (non-inbred) mice. Fetuses showed malformations of the limbs, face, trunk, and tail. There was a statistically significant relationship between the dose of cadmium and the malformation rate. Cadmium (12 mumol/kg ip on day 9) was then given to mice of six inbred strains three of which (A/J, BALB/cJ, and C57BL6J) carry a gene cdm for resistance to cadmium-induced testicular damage, and three strains (AKR/J, CBA/J, and DBA/2J) which do not. Paradoxically, the three strains resistant to cadmium induced testicular damage were significantly more sensitive to its teratogenic effects than were the other three strains. In all inbred strains most malformations involved the limbs. All forelimb defects found in inbred or non-inbred cadmium treated mice were postaxial and indistinguishable from those produced by acetazolamide in mice. The remarkable similarity of the cadmium- and acetazolamide-induced forelimb malformations may be a reflection of the limited number of ways that a rodent forelimb can react to a teratogenic insult. The hindlimb defects were all preaxial."}
{"id": "PMID:473074", "title": "Influence of drinking water -- tap versus purified -- on embryo and fetal development in mice.", "content": "Pregnant CD-1 mice drank either chlorinated tap water obtained at NIEHS from the City of Durham, North Carolina municipal water supply or this same water passed in sequence through an organic removal cartridge and a demineralizer, followed by glass distillation. No significant overall differences in the reproductive status of pregnant mice were noted when the two groups were compared. Similarly, no significant overall influence on the incidence of malformed fetuses could be traced to the purity of drinking water. Month by month comparisons indicated that there were three statistically significant differences; all of which indicated improved performance for the mice that drank tap water. When the results for both groups were combined and a month by month comparison was made there was a significant difference in the pregnancy rate for February (68%) as compared with an overall pregnancy rate of 80% and a 79-93% spread for the other months, but the decrease was due to the low incidence of pregnancy in the group that drank the purified water.", "contents": "Influence of drinking water -- tap versus purified -- on embryo and fetal development in mice. Pregnant CD-1 mice drank either chlorinated tap water obtained at NIEHS from the City of Durham, North Carolina municipal water supply or this same water passed in sequence through an organic removal cartridge and a demineralizer, followed by glass distillation. No significant overall differences in the reproductive status of pregnant mice were noted when the two groups were compared. Similarly, no significant overall influence on the incidence of malformed fetuses could be traced to the purity of drinking water. Month by month comparisons indicated that there were three statistically significant differences; all of which indicated improved performance for the mice that drank tap water. When the results for both groups were combined and a month by month comparison was made there was a significant difference in the pregnancy rate for February (68%) as compared with an overall pregnancy rate of 80% and a 79-93% spread for the other months, but the decrease was due to the low incidence of pregnancy in the group that drank the purified water."}
{"id": "PMID:473075", "title": "Fetal and placental weight relationships in the albino rat near term.", "content": "The relationship of fetal weight to placental weight was examined in 34 albino rats on day 22 of gestation. The influence of maternal weight, fetal position and the number of fetuses in the litter and each uterine horn were assessed also. There was no indication that rats with heavier placentas had heavier or lighter fetuses. However, within each litter, placental weight was weakly correlated (r = 0.297, p less than 0.01) with fetal weight. Maternal weight at mating, although positively related to the number of corpora lutea, was not related to mean fetal or placental weight. The number of fetuses in the litter was negatively related to placental weight but there was no apparent relation to fetal weight. Fetuses and placentas at the ovarian end of the horn were significantly lighter than those at the vaginal end. The strength of the fetal weight:placental weight correlation in the rat is compared to those in other species.", "contents": "Fetal and placental weight relationships in the albino rat near term. The relationship of fetal weight to placental weight was examined in 34 albino rats on day 22 of gestation. The influence of maternal weight, fetal position and the number of fetuses in the litter and each uterine horn were assessed also. There was no indication that rats with heavier placentas had heavier or lighter fetuses. However, within each litter, placental weight was weakly correlated (r = 0.297, p less than 0.01) with fetal weight. Maternal weight at mating, although positively related to the number of corpora lutea, was not related to mean fetal or placental weight. The number of fetuses in the litter was negatively related to placental weight but there was no apparent relation to fetal weight. Fetuses and placentas at the ovarian end of the horn were significantly lighter than those at the vaginal end. The strength of the fetal weight:placental weight correlation in the rat is compared to those in other species."}
{"id": "PMID:473076", "title": "Fetal and placental responses to artificially induced hyperthermia in rats.", "content": "Pregnant rats were utilized to study the effect of maternal hyperthermia on fetal development. Eight groups of six to eight rats were exposed to ambient temperatures of 43-44 degrees C at various stages of pregnancy. All rats were killed on day 20 of gestation. Edema, microencephaly and microphthalmia followed heat treatment on day 4, 6, or 8 and skeletal defects occurred on day 10 of gestation. Apparently heat stress of dams after day 14 of gestation had little or no effect on embryos. Most placentas from day 6-10 treatment groups were significantly heavier than control and exhibited extensive thickening and necrosis of decidua basalis. Our results suggest that the rat is a useful model for investigating maternal hyperthermia as a possible cause of human placentophathies and fetal retardation.", "contents": "Fetal and placental responses to artificially induced hyperthermia in rats. Pregnant rats were utilized to study the effect of maternal hyperthermia on fetal development. Eight groups of six to eight rats were exposed to ambient temperatures of 43-44 degrees C at various stages of pregnancy. All rats were killed on day 20 of gestation. Edema, microencephaly and microphthalmia followed heat treatment on day 4, 6, or 8 and skeletal defects occurred on day 10 of gestation. Apparently heat stress of dams after day 14 of gestation had little or no effect on embryos. Most placentas from day 6-10 treatment groups were significantly heavier than control and exhibited extensive thickening and necrosis of decidua basalis. Our results suggest that the rat is a useful model for investigating maternal hyperthermia as a possible cause of human placentophathies and fetal retardation."}
{"id": "PMID:473077", "title": "Cytogenetics of extragonadal tumors.", "content": "Tissue from six human extragonadal teratomas was obtained for cytogenetic study. These included two gluteal lesions in infant females, and one each of thyroid, mediastinal, sacral and gastric teratomas in males. Tissue culture characteristics indicated tumor growth. All tumors, except the mediastinal lesion showed the presumed karyotype of its host, XX or XY. The mediastinal mass was tetraploid XXYY and contained an X chromatin body and double Y bodies. Our data support the concept that extragonadal teratomas in males and females arise mitotically from diploid cells and are more similar to identical twins. No heterosexual (XX) tumors were seen in males which would be expected in parthenogenetic tumors. The sex chromatin positive tumors seen in earlier studies may have resulted from polyploidy. Thus, male and female extragonadal teratomas have a different origin from the postmeiotic ovarian teratomas.", "contents": "Cytogenetics of extragonadal tumors. Tissue from six human extragonadal teratomas was obtained for cytogenetic study. These included two gluteal lesions in infant females, and one each of thyroid, mediastinal, sacral and gastric teratomas in males. Tissue culture characteristics indicated tumor growth. All tumors, except the mediastinal lesion showed the presumed karyotype of its host, XX or XY. The mediastinal mass was tetraploid XXYY and contained an X chromatin body and double Y bodies. Our data support the concept that extragonadal teratomas in males and females arise mitotically from diploid cells and are more similar to identical twins. No heterosexual (XX) tumors were seen in males which would be expected in parthenogenetic tumors. The sex chromatin positive tumors seen in earlier studies may have resulted from polyploidy. Thus, male and female extragonadal teratomas have a different origin from the postmeiotic ovarian teratomas."}
{"id": "PMID:473080", "title": "Equivalent ages in rat, mouse and chick embryos.", "content": "Data for estimating equivalent ages of rat, mouse and chick embryos are presented, using several sources from the literature. When the time of development of various embryonic structures is matched for the three species, the differences in time for the stages are primarily due to delays in development of the preimplantation blastulas of the rat and mouse in comparison with the incubated chick egg. Development from the blastula stage to completion of major organogenesis proceeds at approximately the same rate for all three species.", "contents": "Equivalent ages in rat, mouse and chick embryos. Data for estimating equivalent ages of rat, mouse and chick embryos are presented, using several sources from the literature. When the time of development of various embryonic structures is matched for the three species, the differences in time for the stages are primarily due to delays in development of the preimplantation blastulas of the rat and mouse in comparison with the incubated chick egg. Development from the blastula stage to completion of major organogenesis proceeds at approximately the same rate for all three species."}
{"id": "PMID:473082", "title": "Prenatal ethanol exposure in mice: teratogenic effects.", "content": "C57BL/6J mice were fed a liquid diet in which 17, 25, or 30% of the calories were derived from ethanol from the fifth through the tenth day of gestation. Control mice were fed lab chow or pair-fed identical diets, except that sucrose substituted isocalorically for ethanol. At term the fetuses were removed and, following fixation, examined by microdissection. The incidence of fetal resorptions and congenital malformations increased in a dose-related manner. Anomalies included skeletal, neurological, urogenital, and cardiovascular systems. These data indicate that in mice, an alcohol diet which is adequate in vitamins and protein results in increased fetal wastage and birth defects.", "contents": "Prenatal ethanol exposure in mice: teratogenic effects. C57BL/6J mice were fed a liquid diet in which 17, 25, or 30% of the calories were derived from ethanol from the fifth through the tenth day of gestation. Control mice were fed lab chow or pair-fed identical diets, except that sucrose substituted isocalorically for ethanol. At term the fetuses were removed and, following fixation, examined by microdissection. The incidence of fetal resorptions and congenital malformations increased in a dose-related manner. Anomalies included skeletal, neurological, urogenital, and cardiovascular systems. These data indicate that in mice, an alcohol diet which is adequate in vitamins and protein results in increased fetal wastage and birth defects."}
{"id": "PMID:473083", "title": "Teratogenic effects of the plant hormone indole-3-acetic acid in mice and rats.", "content": "These studies evaluated the teratogenic potential of indole-3-acetic acid (IAA), a naturally occurring plant hormone, in CF-1 mice and Sprague-Dawley rats. Mice were given 5, 50, 200, or 500 mg IAA/kg/day by gavage on days 7 through 15 of gestation. Rats were given 50, 200, or 500 mg IAA/kg/day by gavage on days 7 through 15 of gestation. IAA was teratogenic in mice and rats at 500 mg/kg/day; cleft palate was induced in both species at this dose level. In mice, other malformations including exencephaly, ablepharia, dilated cerebral ventricles, and crooked tail were also observed. Mice given 500 mg/kg of IAA gained less than control mice during gestation; no evidence of maternal toxicity was observed in rats. IAA did not cause fetal resorptions in either species and was not teratogenic at dose levels below 500 mg/kg.", "contents": "Teratogenic effects of the plant hormone indole-3-acetic acid in mice and rats. These studies evaluated the teratogenic potential of indole-3-acetic acid (IAA), a naturally occurring plant hormone, in CF-1 mice and Sprague-Dawley rats. Mice were given 5, 50, 200, or 500 mg IAA/kg/day by gavage on days 7 through 15 of gestation. Rats were given 50, 200, or 500 mg IAA/kg/day by gavage on days 7 through 15 of gestation. IAA was teratogenic in mice and rats at 500 mg/kg/day; cleft palate was induced in both species at this dose level. In mice, other malformations including exencephaly, ablepharia, dilated cerebral ventricles, and crooked tail were also observed. Mice given 500 mg/kg of IAA gained less than control mice during gestation; no evidence of maternal toxicity was observed in rats. IAA did not cause fetal resorptions in either species and was not teratogenic at dose levels below 500 mg/kg."}
{"id": "PMID:473084", "title": "Gap junctional vesicles in the neural tube of the splotch (Sp) mutant mouse.", "content": "The lumbosacral region of the neural tube was studied by means of transmission electron microscopy in retrospectively confirmed normal (+/+; Sp/+) and abnormal (Sp/Sp) embryos of the splotch mutant mouse early on the ninth day of gestation when the caudal neural groove is normally in the process of closing to form the neural tube. In abnormal embryos, a consistent feature is the presence of gap junctional vesicles, particularly in the region of the neural groove which subsequently fails to close, whereas these structures are rarely observed in similar areas of normal embryos. The possible significance of gap junctional vesicles is discussed in terms of cellular adhesion during early neurogenesis.", "contents": "Gap junctional vesicles in the neural tube of the splotch (Sp) mutant mouse. The lumbosacral region of the neural tube was studied by means of transmission electron microscopy in retrospectively confirmed normal (+/+; Sp/+) and abnormal (Sp/Sp) embryos of the splotch mutant mouse early on the ninth day of gestation when the caudal neural groove is normally in the process of closing to form the neural tube. In abnormal embryos, a consistent feature is the presence of gap junctional vesicles, particularly in the region of the neural groove which subsequently fails to close, whereas these structures are rarely observed in similar areas of normal embryos. The possible significance of gap junctional vesicles is discussed in terms of cellular adhesion during early neurogenesis."}
{"id": "PMID:473085", "title": "The teratogenic activity of a thalidomide analogus EM12 in rats on a low-zinc diet.", "content": "The relationship between the teratogenicity of EM12, 2-(2,6-dioxopiperiden-3'-yl) phthalimidine, a stable analogue of thalidomide, and zinc status in the maternal animal was investigated using pregnant rats on a low-zinc diet (1 ppm zinc, days 0--14 gestation) as the experimental model. Previous studies with this compound in rats fed a commercial diet at oral doses up to 250 mg/kg per day for three days and intravenous doses up to 10 mg/kg per day for three days failed to produce \"typical\" thalidomide malformations. However, when a dose of 150 mg/kg was given intraperitoneally to rats on a low-zinc diet, typical thalidomide malformations occurred with an incidence of 57.5%.", "contents": "The teratogenic activity of a thalidomide analogus EM12 in rats on a low-zinc diet. The relationship between the teratogenicity of EM12, 2-(2,6-dioxopiperiden-3'-yl) phthalimidine, a stable analogue of thalidomide, and zinc status in the maternal animal was investigated using pregnant rats on a low-zinc diet (1 ppm zinc, days 0--14 gestation) as the experimental model. Previous studies with this compound in rats fed a commercial diet at oral doses up to 250 mg/kg per day for three days and intravenous doses up to 10 mg/kg per day for three days failed to produce \"typical\" thalidomide malformations. However, when a dose of 150 mg/kg was given intraperitoneally to rats on a low-zinc diet, typical thalidomide malformations occurred with an incidence of 57.5%."}
{"id": "PMID:473086", "title": "Changes in chemical composition of chick embryos treated with a beta-xyloside and a lathyrogen.", "content": "Nine-day chick embryos were treated with 4-methylumbelliferyl beta-D-xyloside or beta-aminopropionitrile fumarate, and their gross chemical composition was examined one week later. Total DNA was 10--20% less in embryos treated with either drug than it was in control embryos. Xyloside-treated embryos showed marked increases in percent wet weight and in sodium/DNA and chloride/DNA ratios, and small decreases in protein/DNA, hydroxyproline/DNA and sulfate/DNA. None of these parameters was affected in embryos treated with beta-aminopropionitrile. Approximately 85% of the uronic acid of control embryos was present as chondroitin sulfate, with a degree of sulfation of 80% and charge density of 1.8; all of this chondroitin sulfate was covalently linked to peptide and had a number-average molecular weight of 29,300. In embryos treated with beta-xyloside, 90% of the uronic acid was present as chondroitin sulfate, with a degree of sulfation of 40% and charge density ranging from 1 to 2; 27% of this chondroitin sulfate, with an average molecular weight of 25,400, was peptide linked, while 73% was linked to 4-methylumbelliferone and had an average molecular weight of 22,900. The chemical differences between embryos treated with the xyloside and embryos treated with the lathyrogen reinforce the conclusion on morphological grounds that these are distinct syndromes involving different aspects of the extracellular matrix.", "contents": "Changes in chemical composition of chick embryos treated with a beta-xyloside and a lathyrogen. Nine-day chick embryos were treated with 4-methylumbelliferyl beta-D-xyloside or beta-aminopropionitrile fumarate, and their gross chemical composition was examined one week later. Total DNA was 10--20% less in embryos treated with either drug than it was in control embryos. Xyloside-treated embryos showed marked increases in percent wet weight and in sodium/DNA and chloride/DNA ratios, and small decreases in protein/DNA, hydroxyproline/DNA and sulfate/DNA. None of these parameters was affected in embryos treated with beta-aminopropionitrile. Approximately 85% of the uronic acid of control embryos was present as chondroitin sulfate, with a degree of sulfation of 80% and charge density of 1.8; all of this chondroitin sulfate was covalently linked to peptide and had a number-average molecular weight of 29,300. In embryos treated with beta-xyloside, 90% of the uronic acid was present as chondroitin sulfate, with a degree of sulfation of 40% and charge density ranging from 1 to 2; 27% of this chondroitin sulfate, with an average molecular weight of 25,400, was peptide linked, while 73% was linked to 4-methylumbelliferone and had an average molecular weight of 22,900. The chemical differences between embryos treated with the xyloside and embryos treated with the lathyrogen reinforce the conclusion on morphological grounds that these are distinct syndromes involving different aspects of the extracellular matrix."}
{"id": "PMID:473087", "title": "Plastic casts of embryonic respiratory and cardiovascular system: a technique.", "content": "This report describes a technique for producing plastic casts of the cardiovascular and respiratory systems of the chick embryo at Stage 36 or older. For casts of the cardiovascular system, polymerization compound is injected in the right ventricle filling the heart, venous, and arterial system. A two stage injection produced more detailed casts of the pulmonary vasculature. For respiratory system casts, the compound is injected in the trachea filling the bronchi and air sacs. Casts are prepared by tissue corrosion in 10% potassium hydroxide. This technique is an alternative to serial section reconstruction in the study of developing cardiovascular and respiratory system.", "contents": "Plastic casts of embryonic respiratory and cardiovascular system: a technique. This report describes a technique for producing plastic casts of the cardiovascular and respiratory systems of the chick embryo at Stage 36 or older. For casts of the cardiovascular system, polymerization compound is injected in the right ventricle filling the heart, venous, and arterial system. A two stage injection produced more detailed casts of the pulmonary vasculature. For respiratory system casts, the compound is injected in the trachea filling the bronchi and air sacs. Casts are prepared by tissue corrosion in 10% potassium hydroxide. This technique is an alternative to serial section reconstruction in the study of developing cardiovascular and respiratory system."}
{"id": "PMID:473088", "title": "Teratologic evaluation of synthetic delta 9-tetrahydrocannabinol in rabbits.", "content": "Synthetic delta 9-tetrahydrocannabinol (THC) was dissolved in undiluted propylene glycol and administered in daily subcutaneous doses of 15.0, 30.0 or 60.0 mg/kg to pregnant New Zealand white rabbits on days 7--19 of gestation. Maternal food consumption and weight gain were markedly reduced at all dose levels. Embryotoxicity and embryocidal effects were observed in the form of reduced litter weight and number of viable fetuses, respectively, in offspring from pregnant mothers treated with THC. However, on the basis of extensive external, visceral and skeletal examination of all fetuses it may be concluded that THC is not teratogenic in the New Zealand white strain rabbit following subcutaneous administration of doses as high as 60.0 mg/kg/day during the critical period of organogenesis (days 7--19 of gestation). On the other hand, an oral dose of thalidomide (200.0 mg/kg/day), the positive control used in this study, was both embryocidal and teratogenic.", "contents": "Teratologic evaluation of synthetic delta 9-tetrahydrocannabinol in rabbits. Synthetic delta 9-tetrahydrocannabinol (THC) was dissolved in undiluted propylene glycol and administered in daily subcutaneous doses of 15.0, 30.0 or 60.0 mg/kg to pregnant New Zealand white rabbits on days 7--19 of gestation. Maternal food consumption and weight gain were markedly reduced at all dose levels. Embryotoxicity and embryocidal effects were observed in the form of reduced litter weight and number of viable fetuses, respectively, in offspring from pregnant mothers treated with THC. However, on the basis of extensive external, visceral and skeletal examination of all fetuses it may be concluded that THC is not teratogenic in the New Zealand white strain rabbit following subcutaneous administration of doses as high as 60.0 mg/kg/day during the critical period of organogenesis (days 7--19 of gestation). On the other hand, an oral dose of thalidomide (200.0 mg/kg/day), the positive control used in this study, was both embryocidal and teratogenic."}
{"id": "PMID:473091", "title": "Teratogenic potential of inhaled carbon monoxide in mice and rabbits.", "content": "Pregnant CF-1 mice and New Zealand rabbits were exposed to carbon monoxide at a concentration of 250 ppm for 7 or 24 hours daily during the period of major organogenesis, days 6 through 15 of gestation in mice and 6 through 18 of gestation in rabbits. Carboxyhemoglobin levels in the range of 10--15% were observed in both species (control animals had 0.7% or less). Carbon monoxide was not found to be teratogenic in either species. In mice, a significant increase in the incidence of some minor skeletal variants was observed. One litter in each of the carbon monoxide-exposed groups of mice was completely resorbed; none of the litters of control mice or of control or exposed rabbits were completely resorbed. The fetuses of mice exposed to carbon monoxide for seven hours daily were heavier than control fetuses, and those exposed for 24 hours daily were lighter than control fetuses. The reason for this result is not known.", "contents": "Teratogenic potential of inhaled carbon monoxide in mice and rabbits. Pregnant CF-1 mice and New Zealand rabbits were exposed to carbon monoxide at a concentration of 250 ppm for 7 or 24 hours daily during the period of major organogenesis, days 6 through 15 of gestation in mice and 6 through 18 of gestation in rabbits. Carboxyhemoglobin levels in the range of 10--15% were observed in both species (control animals had 0.7% or less). Carbon monoxide was not found to be teratogenic in either species. In mice, a significant increase in the incidence of some minor skeletal variants was observed. One litter in each of the carbon monoxide-exposed groups of mice was completely resorbed; none of the litters of control mice or of control or exposed rabbits were completely resorbed. The fetuses of mice exposed to carbon monoxide for seven hours daily were heavier than control fetuses, and those exposed for 24 hours daily were lighter than control fetuses. The reason for this result is not known."}
{"id": "PMID:473111", "title": "Metabolism of the coagulation factors of the prothrombin complex in hypothyroidism in man.", "content": "The metabolic rate of prothrombin, factors VII, IX and X, was studied in nine hypothyroid patients. Disappearance rates of the four vitamin-K-dependent factors, called the prothrombin complex, were measured after assumedly complete blocking of their synthesis with adequate doses of a coumarin congener (acenocoumarol). Reappearance rates were assessed by induction of synthesis with high doses of vitamin K1 (phytomenadion) when stable hypocoagulability had been achieved. Normal values for these rates were derived from earlier studies in our laboratory. In hypothyroid patients the rates of both disappearance and reappearance were significantly slower for all factors tested. Practical consequences of these observations are discussed. The initial level of factor-IX activity in all nine patients was substantially lower than in normal individuals. Therapy by thyroxine-substitution led to normal levels of factor-IX. This implies a divergency in the retardation of the breakdown and production rates in hypothyroidism. The reappearance rate was indeed found to be more retarded than the disappearance rate.", "contents": "Metabolism of the coagulation factors of the prothrombin complex in hypothyroidism in man. The metabolic rate of prothrombin, factors VII, IX and X, was studied in nine hypothyroid patients. Disappearance rates of the four vitamin-K-dependent factors, called the prothrombin complex, were measured after assumedly complete blocking of their synthesis with adequate doses of a coumarin congener (acenocoumarol). Reappearance rates were assessed by induction of synthesis with high doses of vitamin K1 (phytomenadion) when stable hypocoagulability had been achieved. Normal values for these rates were derived from earlier studies in our laboratory. In hypothyroid patients the rates of both disappearance and reappearance were significantly slower for all factors tested. Practical consequences of these observations are discussed. The initial level of factor-IX activity in all nine patients was substantially lower than in normal individuals. Therapy by thyroxine-substitution led to normal levels of factor-IX. This implies a divergency in the retardation of the breakdown and production rates in hypothyroidism. The reappearance rate was indeed found to be more retarded than the disappearance rate."}
{"id": "PMID:473112", "title": "The frequency of bleeding and height of adolescent haemophiliacs.", "content": "The relation between the height of adolescent haemophiliacs and their bleeding frequency has been studied. 45 haemophiliacs aged 10--19 years were divided into 3 groups: small, medium and tall, using a Height Standard Deviation Score. The average bleeding frequency per 100 days in the group of small haemophiliacs was 8.71 +/- SD 4.47, in the medium height group 10.18 +/- SD 6.71, while the tall individuals bled in average 15.97 +/- SD 3.15 every 100 days. There was no relationship between age and bleeding frequency.", "contents": "The frequency of bleeding and height of adolescent haemophiliacs. The relation between the height of adolescent haemophiliacs and their bleeding frequency has been studied. 45 haemophiliacs aged 10--19 years were divided into 3 groups: small, medium and tall, using a Height Standard Deviation Score. The average bleeding frequency per 100 days in the group of small haemophiliacs was 8.71 +/- SD 4.47, in the medium height group 10.18 +/- SD 6.71, while the tall individuals bled in average 15.97 +/- SD 3.15 every 100 days. There was no relationship between age and bleeding frequency."}
{"id": "PMID:473113", "title": "Factor VIII inhibitor and bronchogenic carcinoma.", "content": "An inhibitor to procoagulant factor VIII (FVIIIC) developed in a patient three years after palliative resection of a bronchogenic carcinoma. The inhibitor was not active against ristocetin cofactor but possibly had some activity against factor XI. It responded to immunosuppressive therapy. This is apparently the first reported association of carcinoma and factor VIII inhibitor.", "contents": "Factor VIII inhibitor and bronchogenic carcinoma. An inhibitor to procoagulant factor VIII (FVIIIC) developed in a patient three years after palliative resection of a bronchogenic carcinoma. The inhibitor was not active against ristocetin cofactor but possibly had some activity against factor XI. It responded to immunosuppressive therapy. This is apparently the first reported association of carcinoma and factor VIII inhibitor."}
{"id": "PMID:473114", "title": "Kinetic aspects of the interaction of blood clotting enzymes. VIII. The relation between clotting time and clotting velocity.", "content": "It is shown that in a system where thrombin acts on fibrinogen, the clotting time can be used to assess coagulation velocity because clotting time and clotting velocity are inversely proportional.", "contents": "Kinetic aspects of the interaction of blood clotting enzymes. VIII. The relation between clotting time and clotting velocity. It is shown that in a system where thrombin acts on fibrinogen, the clotting time can be used to assess coagulation velocity because clotting time and clotting velocity are inversely proportional."}
{"id": "PMID:473115", "title": "Thrombin-like and factor X-activator components of Bothrops snake venoms.", "content": "A comparative study on the coagulant activity of snake venoms was carried out in 26 Bothrops species, using specific clotting systems for the thrombin-like and the factor X-activator activities. With only two exceptions (B. erythromelas and B. castelnaudi) all venoms showed thrombin-like activity, since they were able to clot fibrinogen directly. The absence of thrombin-like action of B. erythromelas venom is due to a fibrinogenolytic effect. Five venoms (B. atrox asper, B. bilineatus bilineatus, B. cotiara, B. fonsecai and B. itapetiningae) were unable to produce a prothrombin activator when preincubated with serum, factor V, and phospholipid. None of the venoms seems to require factors VII, VIII, IX, XII and XIII for their complete coagulant action. Direct prothrombin activation was observed in most of the Bothrops venoms, alone or combined with thrombin-like and factor X-activator activities. An anticoagulant activity was exhibited by B. castelnaudi venom, probably due to an anti-Xa action. This study points out that the coagulant activity of snake venoms varies within the same genus and must be characterized for each species. Thus, in Bothrops venoms the thrombin-like and factor X-activator components are not always associated, the coagulant effect may be related only to one of the components.", "contents": "Thrombin-like and factor X-activator components of Bothrops snake venoms. A comparative study on the coagulant activity of snake venoms was carried out in 26 Bothrops species, using specific clotting systems for the thrombin-like and the factor X-activator activities. With only two exceptions (B. erythromelas and B. castelnaudi) all venoms showed thrombin-like activity, since they were able to clot fibrinogen directly. The absence of thrombin-like action of B. erythromelas venom is due to a fibrinogenolytic effect. Five venoms (B. atrox asper, B. bilineatus bilineatus, B. cotiara, B. fonsecai and B. itapetiningae) were unable to produce a prothrombin activator when preincubated with serum, factor V, and phospholipid. None of the venoms seems to require factors VII, VIII, IX, XII and XIII for their complete coagulant action. Direct prothrombin activation was observed in most of the Bothrops venoms, alone or combined with thrombin-like and factor X-activator activities. An anticoagulant activity was exhibited by B. castelnaudi venom, probably due to an anti-Xa action. This study points out that the coagulant activity of snake venoms varies within the same genus and must be characterized for each species. Thus, in Bothrops venoms the thrombin-like and factor X-activator components are not always associated, the coagulant effect may be related only to one of the components."}
{"id": "PMID:473116", "title": "Effect of sodium chloride on limulus amebocyte lysate. Inhibition of endotoxin activation of procoagulase.", "content": "Concentrations of sodium chloride up to 3 M increase the time necessary for the clot formation from Limulus amebocyte lysate (LAL) induced with endotoxin. Sodium chloride at a concentration of 4 M prevents clot formation by either precipitation or denaturation of procoagulase. The time necessary for the activation of procoagulase by endotoxin is increased by a change in the sodium chloride concentration from 0.15 M to 0.588 M. No effect on the proteolytic phase or the polymerization phase of the clotting reaction is detected by the increase in sodium chloride concentration from 0.15 M to 0.588 M. The authors conclude that increased sodium chloride concentrations may aid the isolation of procoagulase.", "contents": "Effect of sodium chloride on limulus amebocyte lysate. Inhibition of endotoxin activation of procoagulase. Concentrations of sodium chloride up to 3 M increase the time necessary for the clot formation from Limulus amebocyte lysate (LAL) induced with endotoxin. Sodium chloride at a concentration of 4 M prevents clot formation by either precipitation or denaturation of procoagulase. The time necessary for the activation of procoagulase by endotoxin is increased by a change in the sodium chloride concentration from 0.15 M to 0.588 M. No effect on the proteolytic phase or the polymerization phase of the clotting reaction is detected by the increase in sodium chloride concentration from 0.15 M to 0.588 M. The authors conclude that increased sodium chloride concentrations may aid the isolation of procoagulase."}
{"id": "PMID:473117", "title": "Interactions between human plasma proteins and heparin-poly(methyl methacrylate) copolymer.", "content": "A solid Heparin-PMMA copolymer has been synthetized by a radical polymerization of methyl methacrylate from oxidative reaction initiated by Ce4+ ions in the presence of heparin. Covalently linked heparin was 10% of copolymer weight. The antithrombin activity of the copolymer corresponded to 1% of grafted heparin. PMMA sequence of the copolymer played the leading role in fibrinogen, immunoglobulins, transferrin and albumin adsorption. These proteins adsorbed on the copolymer, showed different competitive desorption pattern in the presence of whole plasma: fibrinogen presented the highest degree of affinity for the copolymer. The heparin part of the copolymer was responsible for antithrombin III adsorption and for decrease of factor V activity. Active antithrombin III was eluted. An inactivation of factor V in plasma was observed using high concentrations of soluble heparin. This result suggested that copolymer heparin chains, even devoid of antithrombin activity were involved in this inactivation. With Heparin-PMMA copolymer, plasma clotting pro-enzymes behaved differently than on heparin-sepharose copolymer:disappearance of factor XI activity, decrease in prekallikrein activity and activation of factor IX were observed. PMMA sequences were responsible for factor IX activation.", "contents": "Interactions between human plasma proteins and heparin-poly(methyl methacrylate) copolymer. A solid Heparin-PMMA copolymer has been synthetized by a radical polymerization of methyl methacrylate from oxidative reaction initiated by Ce4+ ions in the presence of heparin. Covalently linked heparin was 10% of copolymer weight. The antithrombin activity of the copolymer corresponded to 1% of grafted heparin. PMMA sequence of the copolymer played the leading role in fibrinogen, immunoglobulins, transferrin and albumin adsorption. These proteins adsorbed on the copolymer, showed different competitive desorption pattern in the presence of whole plasma: fibrinogen presented the highest degree of affinity for the copolymer. The heparin part of the copolymer was responsible for antithrombin III adsorption and for decrease of factor V activity. Active antithrombin III was eluted. An inactivation of factor V in plasma was observed using high concentrations of soluble heparin. This result suggested that copolymer heparin chains, even devoid of antithrombin activity were involved in this inactivation. With Heparin-PMMA copolymer, plasma clotting pro-enzymes behaved differently than on heparin-sepharose copolymer:disappearance of factor XI activity, decrease in prekallikrein activity and activation of factor IX were observed. PMMA sequences were responsible for factor IX activation."}
{"id": "PMID:473119", "title": "Triglyceride lipase activity and human platelets.", "content": "The activity of triglyceride lipases in human postheparin plasma is significantly higher in platelet rich than platelet poor plasma. This holds for total activity, lipoprotein lipase (LPL) activity, and hepatic triglyceride lipase (H-TGL) activity. Gel filtration of platelet rich postheparin plasma on Sepharose 2 B will separate platelets from triglyceride lipase activity. The very small triglyceride lipase activity of isolated platelets is inhibited by 1.0 M NaCl, slightly inhibited by specific antibody to hepatic lipase, and not influenced by specific antibody to lipoprotein lipase.", "contents": "Triglyceride lipase activity and human platelets. The activity of triglyceride lipases in human postheparin plasma is significantly higher in platelet rich than platelet poor plasma. This holds for total activity, lipoprotein lipase (LPL) activity, and hepatic triglyceride lipase (H-TGL) activity. Gel filtration of platelet rich postheparin plasma on Sepharose 2 B will separate platelets from triglyceride lipase activity. The very small triglyceride lipase activity of isolated platelets is inhibited by 1.0 M NaCl, slightly inhibited by specific antibody to hepatic lipase, and not influenced by specific antibody to lipoprotein lipase."}
{"id": "PMID:473144", "title": "[Is anaemia of veal calves normochromic or hypochromic? (author's transl)].", "content": "The anaemia resulting from iron deficiency is described as hypochromic in the medical literature. In the veterinary literature, anaemia of veal calves is usually regraded as normochromic. In order to examine the correctness of this view, the present authors studied the Hb and haematocrit (Hc) levels of the blood of veal calves in a comparative feeding trial for sixteen weeks. Three groups of male Dutch-Friesian calves, each group consisting of approximately thirty animals, were fed a milk replacer which contained 5, 10 or 25 ppm of iron respectively. The calculated mean Hb-concentration in the erythrocytes of the animals given 5 and 10 ppm of iron dropped during the trial period to below levels occurring at the age of two weeks. In the group given 25 ppm of iron, the erythrocyte Hb showed a rapid increase from the age of ten weeks and continuing throughout the rest of the trial period. At the age of sixteen weeks, the groups showed significant differences (P less than 0.01) in their mean erythrocyte Hb-concentrations. In veal calves, fed milkreplacers having an iron content below 25 ppm of Fe, the anaemia therefore is hypochromic.", "contents": "[Is anaemia of veal calves normochromic or hypochromic? (author's transl)]. The anaemia resulting from iron deficiency is described as hypochromic in the medical literature. In the veterinary literature, anaemia of veal calves is usually regraded as normochromic. In order to examine the correctness of this view, the present authors studied the Hb and haematocrit (Hc) levels of the blood of veal calves in a comparative feeding trial for sixteen weeks. Three groups of male Dutch-Friesian calves, each group consisting of approximately thirty animals, were fed a milk replacer which contained 5, 10 or 25 ppm of iron respectively. The calculated mean Hb-concentration in the erythrocytes of the animals given 5 and 10 ppm of iron dropped during the trial period to below levels occurring at the age of two weeks. In the group given 25 ppm of iron, the erythrocyte Hb showed a rapid increase from the age of ten weeks and continuing throughout the rest of the trial period. At the age of sixteen weeks, the groups showed significant differences (P less than 0.01) in their mean erythrocyte Hb-concentrations. In veal calves, fed milkreplacers having an iron content below 25 ppm of Fe, the anaemia therefore is hypochromic."}
{"id": "PMID:473145", "title": "[Traumatic pericarditis, an old acquaintance in due form (author's transl)].", "content": "The clinical, radiographic and post-mortem studies in an 18-month-old heifer are reviewed. The complaint was that the animal had not been eating and drinking for as long as ten days. Clinical studies revealed obvious changes of the respiratory and circulatory systems: marked engorgement of the jugular veins, clapotement synchronous with the heart beat and bronchial respiration over the apical lobes. Clinical examination was followed by a differential diagnosis in which traumatic pericarditis, pleurisy and an intrathoracic abscess were mainly considered, and is was decided to carry out radiography. The X-rays showed a major lesion, within which a horizontal level was observed. In addition, a large foreign body was discernible. The animal died within a few days and the diagnosis of traumatic pericarditis was verified, though pleurisy was also found to be present. The ichorous splenitis revealed by the post-mortem studies was not observed on clinical examination. The difficulties involved in arriving at a correct differentiation between pericarditis, pleuristy and an abcess are discussed in retrospect.", "contents": "[Traumatic pericarditis, an old acquaintance in due form (author's transl)]. The clinical, radiographic and post-mortem studies in an 18-month-old heifer are reviewed. The complaint was that the animal had not been eating and drinking for as long as ten days. Clinical studies revealed obvious changes of the respiratory and circulatory systems: marked engorgement of the jugular veins, clapotement synchronous with the heart beat and bronchial respiration over the apical lobes. Clinical examination was followed by a differential diagnosis in which traumatic pericarditis, pleurisy and an intrathoracic abscess were mainly considered, and is was decided to carry out radiography. The X-rays showed a major lesion, within which a horizontal level was observed. In addition, a large foreign body was discernible. The animal died within a few days and the diagnosis of traumatic pericarditis was verified, though pleurisy was also found to be present. The ichorous splenitis revealed by the post-mortem studies was not observed on clinical examination. The difficulties involved in arriving at a correct differentiation between pericarditis, pleuristy and an abcess are discussed in retrospect."}
{"id": "PMID:473149", "title": "An investigation of the pathology of mycoplasma mastitis in the cow.", "content": "A field case of mastitis in cows, caused by Mycoplasma agalactiae var. bovis, formed the occasion to conduct an infection experiment. Five lactating heifers were infected in the udder at different times. The cows were slaughtered 2, 5, 7, 9 and 12 days p.i. and the pathological changes were studied. The investigation indicated that the pathological picture differed with time: in the acute stage, the inflammation was characterized by exudation of mostly eosinophils in the alveoli; later on, the mastitis was identified by an interstitial reaction with eosinophils and mononuclear cells, including plasma cells and lymphocytes; in the chronic stage, progressive fibroplasia around ductuli and alveoli, with hypertrophy of alveolar epithelium, was characteristic. The pathological findings are discussed.", "contents": "An investigation of the pathology of mycoplasma mastitis in the cow. A field case of mastitis in cows, caused by Mycoplasma agalactiae var. bovis, formed the occasion to conduct an infection experiment. Five lactating heifers were infected in the udder at different times. The cows were slaughtered 2, 5, 7, 9 and 12 days p.i. and the pathological changes were studied. The investigation indicated that the pathological picture differed with time: in the acute stage, the inflammation was characterized by exudation of mostly eosinophils in the alveoli; later on, the mastitis was identified by an interstitial reaction with eosinophils and mononuclear cells, including plasma cells and lymphocytes; in the chronic stage, progressive fibroplasia around ductuli and alveoli, with hypertrophy of alveolar epithelium, was characteristic. The pathological findings are discussed."}
{"id": "PMID:473150", "title": "Severe extrapyramidal syndrome in a dog caused by a haloperidol (Serenase) intoxication.", "content": "The case history of a one-year-old male mongrel dog intoxicated with 120 mg haloperidol is described. The dog showed a coma with a severe extrapyramidal syndrome and was treated with orphenadrine. Symptoms, occurrence, and therapy of the extrapyrmidal syndrome are discussed. Emphasis is laid on the importance to differentiate this syndrome from epilepsy and other neurological disorders.", "contents": "Severe extrapyramidal syndrome in a dog caused by a haloperidol (Serenase) intoxication. The case history of a one-year-old male mongrel dog intoxicated with 120 mg haloperidol is described. The dog showed a coma with a severe extrapyramidal syndrome and was treated with orphenadrine. Symptoms, occurrence, and therapy of the extrapyrmidal syndrome are discussed. Emphasis is laid on the importance to differentiate this syndrome from epilepsy and other neurological disorders."}
{"id": "PMID:473151", "title": "Induction of parturition in the sow with a prostaglandin analogue (I.C.I. 80996).", "content": "Parturition was induced in 112 gilts and sows on day 111,, 112, and 113 of gestation by means of a single intramuscular injection of 175 mcg of a prostaglandin F2 alpha analogue (Cloprostenol, I.C.I 80996). No side effects were detected immediately after injection and the course of the induced parturition was normal. The interval between injection and parturition was approximately 28 hours. Induction of parturition on day 113 resulted in a significant shortening of this interval as compared with day 111, and 112. The average weights of the piglets at birth and at 5 weeks were within the normal range. The percentage of stillbirths and the loss of piglets up to weaning did not differ significantly between control and experimental groups. The practical applications of induction of parturition are discussed.", "contents": "Induction of parturition in the sow with a prostaglandin analogue (I.C.I. 80996). Parturition was induced in 112 gilts and sows on day 111,, 112, and 113 of gestation by means of a single intramuscular injection of 175 mcg of a prostaglandin F2 alpha analogue (Cloprostenol, I.C.I 80996). No side effects were detected immediately after injection and the course of the induced parturition was normal. The interval between injection and parturition was approximately 28 hours. Induction of parturition on day 113 resulted in a significant shortening of this interval as compared with day 111, and 112. The average weights of the piglets at birth and at 5 weeks were within the normal range. The percentage of stillbirths and the loss of piglets up to weaning did not differ significantly between control and experimental groups. The practical applications of induction of parturition are discussed."}
{"id": "PMID:473152", "title": "Nutritional and metabolic aspects of fatty liver disease in poultry.", "content": "There are two metabolic disorders of major commercial importance in poultry that involve the occurrence of fatty deposits in the liver. Fatty Liver and Kidney Syndrome (FLKS) affects young birds and the main manifestations, lipid infiltrations into liver and many other organs, are apparently secondary effects of the primary lesion that lies in carbohydrate metabolism. Although several nutritional and environmental factors influence FLKS, the main factor is the vitamin, biotin. In the absence of an adequate supply of biotin, the hepatic activity of pyruvate carboxylase, a biotin-dependent enzyme, becomes so low that gluconeogenesis in the liver via pyruvate becomes negligible. When the bird is then subject to a mild stress and/or short term fasting, liver glycogen reserves become rapidly depleted and a progressive hypoglycaemia develops that ultimatley proves fatal. Supplementing diets with adequate amounts of biotin can prevent the syndrome. Fatty Liver Haemorrhagic Syndrome (FLHS) is brought about by an excessive accumulation of fat in the livers of adult hens which weakens the cellular structure of the liver and allows fatal haemorrhaging to occur. The aetiology of the syndrome is not clear, but a major factor is an excessive intake of dietary energy. However, the involvement of hormonal and toxicological factors, as well as other nutritional factors, is also possible.", "contents": "Nutritional and metabolic aspects of fatty liver disease in poultry. There are two metabolic disorders of major commercial importance in poultry that involve the occurrence of fatty deposits in the liver. Fatty Liver and Kidney Syndrome (FLKS) affects young birds and the main manifestations, lipid infiltrations into liver and many other organs, are apparently secondary effects of the primary lesion that lies in carbohydrate metabolism. Although several nutritional and environmental factors influence FLKS, the main factor is the vitamin, biotin. In the absence of an adequate supply of biotin, the hepatic activity of pyruvate carboxylase, a biotin-dependent enzyme, becomes so low that gluconeogenesis in the liver via pyruvate becomes negligible. When the bird is then subject to a mild stress and/or short term fasting, liver glycogen reserves become rapidly depleted and a progressive hypoglycaemia develops that ultimatley proves fatal. Supplementing diets with adequate amounts of biotin can prevent the syndrome. Fatty Liver Haemorrhagic Syndrome (FLHS) is brought about by an excessive accumulation of fat in the livers of adult hens which weakens the cellular structure of the liver and allows fatal haemorrhaging to occur. The aetiology of the syndrome is not clear, but a major factor is an excessive intake of dietary energy. However, the involvement of hormonal and toxicological factors, as well as other nutritional factors, is also possible."}
{"id": "PMID:473153", "title": "Haemadsorption inhibition test for the identification of Mycoplasma gallisepticum and Mycoplasma synoviae.", "content": "Colonies of the avian mycoplasma strains Mycoplasma gallisepticum S6 and Mycoplasma synoviae WVU 1853 and two Mycoplasma synoviae isolates from this laboratory were shown to be haemadsorption positive for chicken erythrocytes. Three Mycoplasma synoviae isolates from this laboratory proved to be haemadsorption and haemagglutination negative. The haemadsorption of the mycoplasma colonies mentioned above was inhibited with specific antisera of either high or low titre. No cross-inhibition was observed. It is suggested that this test could be used for a quick tentative identification of the two avian mycoplasmas on primary solid-medium cultures.", "contents": "Haemadsorption inhibition test for the identification of Mycoplasma gallisepticum and Mycoplasma synoviae. Colonies of the avian mycoplasma strains Mycoplasma gallisepticum S6 and Mycoplasma synoviae WVU 1853 and two Mycoplasma synoviae isolates from this laboratory were shown to be haemadsorption positive for chicken erythrocytes. Three Mycoplasma synoviae isolates from this laboratory proved to be haemadsorption and haemagglutination negative. The haemadsorption of the mycoplasma colonies mentioned above was inhibited with specific antisera of either high or low titre. No cross-inhibition was observed. It is suggested that this test could be used for a quick tentative identification of the two avian mycoplasmas on primary solid-medium cultures."}
{"id": "PMID:473154", "title": "Common diseases of pet animals in West Africa.", "content": "A brief review of the major disease of exotic pet animals in West Africa is presented, based on experience, mainly in Nigeria; with pet animals imported from Europe. The conditions mentioned are: ticks, and tickborne diseases, rabies, distemper, feline panleukopenia, trypanosomiasis, hookworm and tumbu-fly infections. The control measures before departure are emphasized.", "contents": "Common diseases of pet animals in West Africa. A brief review of the major disease of exotic pet animals in West Africa is presented, based on experience, mainly in Nigeria; with pet animals imported from Europe. The conditions mentioned are: ticks, and tickborne diseases, rabies, distemper, feline panleukopenia, trypanosomiasis, hookworm and tumbu-fly infections. The control measures before departure are emphasized."}
{"id": "PMID:473156", "title": "The structure of A segments from glycerinated skeletal muscle.", "content": "The A band of skeletal muscle consists of an array of thick myosin-containing filaments along with non-myosin proteins such as C protein and M line protein. In order to study the arrangement of the myosin and non-myosin components, A segments which are aggregations of thick filaments held together at the M line were prepared from glycerinated chicken pectoral and rabbit psoas muscles and examined by electron microscopy. Details of the preparative technique and comparison of the morphologies of A segments and I segments are provided. The A segments from chicken pectoral muscle exhibited 11 to 12 stripes in each half lateral to the bare zone. Several less distinct bands as well as subdivisions of the individual stripes were also observed. The periodicity of the major stripes in the A segments was 424 +/- 10 A. The A segments prepared from rabbit psoas muscle had a periodicity of 432 +/- 13 A, but in contrast with chicken A segments, fewer rabbit A segments showed this periodicity. We conclude that A segments can be separated from glycerinated chicken and rabbit skeletal muscles and compare our results with those of others who prepared A segments from frog and rabbit skeletal muscles in the absence of glycerol.", "contents": "The structure of A segments from glycerinated skeletal muscle. The A band of skeletal muscle consists of an array of thick myosin-containing filaments along with non-myosin proteins such as C protein and M line protein. In order to study the arrangement of the myosin and non-myosin components, A segments which are aggregations of thick filaments held together at the M line were prepared from glycerinated chicken pectoral and rabbit psoas muscles and examined by electron microscopy. Details of the preparative technique and comparison of the morphologies of A segments and I segments are provided. The A segments from chicken pectoral muscle exhibited 11 to 12 stripes in each half lateral to the bare zone. Several less distinct bands as well as subdivisions of the individual stripes were also observed. The periodicity of the major stripes in the A segments was 424 +/- 10 A. The A segments prepared from rabbit psoas muscle had a periodicity of 432 +/- 13 A, but in contrast with chicken A segments, fewer rabbit A segments showed this periodicity. We conclude that A segments can be separated from glycerinated chicken and rabbit skeletal muscles and compare our results with those of others who prepared A segments from frog and rabbit skeletal muscles in the absence of glycerol."}
{"id": "PMID:473157", "title": "Aggregates of particles on the plasmalemma of striated muscle from a spider.", "content": "Aggregates of large intramembranous particles are described in the surface membrane of muscle fibers of a spider. The particles are continuous with extensions into the extracellular space, forming large complexes. No intramembranous particles are visible at sites of Z line attachment to the plasma membrane.", "contents": "Aggregates of particles on the plasmalemma of striated muscle from a spider. Aggregates of large intramembranous particles are described in the surface membrane of muscle fibers of a spider. The particles are continuous with extensions into the extracellular space, forming large complexes. No intramembranous particles are visible at sites of Z line attachment to the plasma membrane."}
{"id": "PMID:473158", "title": "Fine structure of muscle and other components of the trunk of Sagitta setosa (Chaetognath).", "content": "The histology of the Chaetognath's trunk is largely based upon works of Hertwig (1880) and Burfield (1927) and is revised here essentially on ultrastructural basis, in a study on Sagitta setosa. The trunk is composed of a thick multilayered epidermis, without cuticle, and contains an abundant nervous system. It is separated from the underlying general musculature by a \"basement membrane\" which is not crossed by nerves fibres in the region of the ventral ganglion. We have been mainly interested in the muscular tissue. It has no close contact with the body cavity but is surrounded by a thin monolayer epithelium. The general musculature is composed of two types of muscles. The first one, forming the primary musculature, is divided by mesenteries and lateral fields into four quadrants. It contains groups of fibres (C then A) alternating with other groups of fibres (B). The second one, constituting the secondary musculature, is divided into four bands. Both types of fibres are readily identifiable by their myofibrils. The fibres of primary musculature, in close contact with their membrane level, have no basement lamina except at the myoepidermic junctions.", "contents": "Fine structure of muscle and other components of the trunk of Sagitta setosa (Chaetognath). The histology of the Chaetognath's trunk is largely based upon works of Hertwig (1880) and Burfield (1927) and is revised here essentially on ultrastructural basis, in a study on Sagitta setosa. The trunk is composed of a thick multilayered epidermis, without cuticle, and contains an abundant nervous system. It is separated from the underlying general musculature by a \"basement membrane\" which is not crossed by nerves fibres in the region of the ventral ganglion. We have been mainly interested in the muscular tissue. It has no close contact with the body cavity but is surrounded by a thin monolayer epithelium. The general musculature is composed of two types of muscles. The first one, forming the primary musculature, is divided by mesenteries and lateral fields into four quadrants. It contains groups of fibres (C then A) alternating with other groups of fibres (B). The second one, constituting the secondary musculature, is divided into four bands. Both types of fibres are readily identifiable by their myofibrils. The fibres of primary musculature, in close contact with their membrane level, have no basement lamina except at the myoepidermic junctions."}
{"id": "PMID:473159", "title": "Organ culture of adult amphibian heart: a fine structural analysis.", "content": "Pieces of hearts from adult newts were cultured up to 2 months. Within 7 days of culture, approximately 37% of the cardiac explants were attached to the substrate and more than 33% of the attached explants and approximately 15% of the unattached explants established pulsation rates ranging from 3 to 67 beats/min. The control and cultured explants were processed at weekly intervals for electron microscopy. The diameter of the control cardiac muscle cells ranged approximately 3-5 micron. The cell surface was provided with microvilli. The intercellular spaces ranged approximately 150-500 A. The intercalated discs lacked the step-like courses observed in the mammalian cardiac muscle. Sarcoplasmic reticulum was scanty. Desmosomal-dense materials were frequently continuous with the Z-bands of both control and cultured cardiac muscle cells. The transverse tubular system and gap junction were absent in newt ventricles. The functional implications of these characterisitics are discussed. At the end of 1 week of culture, the surfaces of the explants were covered by one or more layers of non-muscle cells, and the core of the explants consisted mostly of cardiac muscle cells. In a few cardiac muscle cells the myofibrillar organization was disrupted, resulting in the distribution of scattered patches of myofibrils and free myofilaments in the sarcoplasm. A small number of intact muscle cells contained a considerable number of dense granules in the sarcoplasm. At 15 days in culture, a large number of muscle cells showed structural features reminiscent of embryonic cardiac muscle cells. These cells possessed patches of myofibrils, scattered myofilaments and scanty sarcoplasmic reticulum along with other cellular organelles and inclusions. Several of these altered cardiac muscle cells contained mitotic figures. The cardiac explants maintained the initial beating rate until the end of 2 months of culture, except for the 11% of the explants which stopped beating. By 3-4 weeks in culture, most of the cardiac muscle cells possessed the altered cell morphology mentioned above. The explants after 60 days in culture became more flattened than the earlier explants. The intact cardiac muscle cells were rare, and the cores of the explants were mostly occupied by the altered cardiac muscle cells. It is evident from our studies that the cardiac muscle cells have undergone dedifferentiation in long-term culture, and that this dedifferentiation process has yet had no effect in the maintenance of contractility of the explants. Furthermore, these dedifferentiated cardiac muscle cells are capable of DNA synthesis and mitosis.", "contents": "Organ culture of adult amphibian heart: a fine structural analysis. Pieces of hearts from adult newts were cultured up to 2 months. Within 7 days of culture, approximately 37% of the cardiac explants were attached to the substrate and more than 33% of the attached explants and approximately 15% of the unattached explants established pulsation rates ranging from 3 to 67 beats/min. The control and cultured explants were processed at weekly intervals for electron microscopy. The diameter of the control cardiac muscle cells ranged approximately 3-5 micron. The cell surface was provided with microvilli. The intercellular spaces ranged approximately 150-500 A. The intercalated discs lacked the step-like courses observed in the mammalian cardiac muscle. Sarcoplasmic reticulum was scanty. Desmosomal-dense materials were frequently continuous with the Z-bands of both control and cultured cardiac muscle cells. The transverse tubular system and gap junction were absent in newt ventricles. The functional implications of these characterisitics are discussed. At the end of 1 week of culture, the surfaces of the explants were covered by one or more layers of non-muscle cells, and the core of the explants consisted mostly of cardiac muscle cells. In a few cardiac muscle cells the myofibrillar organization was disrupted, resulting in the distribution of scattered patches of myofibrils and free myofilaments in the sarcoplasm. A small number of intact muscle cells contained a considerable number of dense granules in the sarcoplasm. At 15 days in culture, a large number of muscle cells showed structural features reminiscent of embryonic cardiac muscle cells. These cells possessed patches of myofibrils, scattered myofilaments and scanty sarcoplasmic reticulum along with other cellular organelles and inclusions. Several of these altered cardiac muscle cells contained mitotic figures. The cardiac explants maintained the initial beating rate until the end of 2 months of culture, except for the 11% of the explants which stopped beating. By 3-4 weeks in culture, most of the cardiac muscle cells possessed the altered cell morphology mentioned above. The explants after 60 days in culture became more flattened than the earlier explants. The intact cardiac muscle cells were rare, and the cores of the explants were mostly occupied by the altered cardiac muscle cells. It is evident from our studies that the cardiac muscle cells have undergone dedifferentiation in long-term culture, and that this dedifferentiation process has yet had no effect in the maintenance of contractility of the explants. Furthermore, these dedifferentiated cardiac muscle cells are capable of DNA synthesis and mitosis."}
{"id": "PMID:473160", "title": "Fine structure of the cuticle of the desert scorpion, Hadrurus arizonensis.", "content": "The structure of the sclerite and intersegmental cuticle of the opithosoma of the desert scorpion, Hadrurus arizonensis, has been examined by transmission electron microscopy. The sclerite cuticle contains a four-layered epicuticle, a hyaline exocuticle, an inner exocuticle and an endocuticle. The outer part of the hyaline exocuticle and the whole of the inner exocuticle are constructed of helicoidally arranged planes of microfibrils. Within the endocuticle, the overall architecture is not helicoidal as previously assumed, but consists of bundles of microfibrils oriented horizontally and vertically. Microbibrils of the inner exocuticle and the endocutile are seen as simple unstained rods, but those of the hyaline exocuticle are electron dense rods with an unstained central core. The intersegmental cuticle contains a four-layered epicuticle and a procuticle. In detail, its fine structure differs in most respects from that of the sclerite cuticle. Electron microscopy reveals that hyaline exocuticle, previously assumed to be continuous from sclerite to intersegmental membrane, is absent in the latter.", "contents": "Fine structure of the cuticle of the desert scorpion, Hadrurus arizonensis. The structure of the sclerite and intersegmental cuticle of the opithosoma of the desert scorpion, Hadrurus arizonensis, has been examined by transmission electron microscopy. The sclerite cuticle contains a four-layered epicuticle, a hyaline exocuticle, an inner exocuticle and an endocuticle. The outer part of the hyaline exocuticle and the whole of the inner exocuticle are constructed of helicoidally arranged planes of microfibrils. Within the endocuticle, the overall architecture is not helicoidal as previously assumed, but consists of bundles of microfibrils oriented horizontally and vertically. Microbibrils of the inner exocuticle and the endocutile are seen as simple unstained rods, but those of the hyaline exocuticle are electron dense rods with an unstained central core. The intersegmental cuticle contains a four-layered epicuticle and a procuticle. In detail, its fine structure differs in most respects from that of the sclerite cuticle. Electron microscopy reveals that hyaline exocuticle, previously assumed to be continuous from sclerite to intersegmental membrane, is absent in the latter."}
{"id": "PMID:473161", "title": "Fine structure of the epicuticle of the desert scorpion, Hadrurus arizonensis, with reference to location of lipids.", "content": "The surface and transverse sections of the epicuticle of the desert scorpion, Hadrurus arizonensis, were examined by scanning and transmission electron microscopy, respectively. Sclerite cuticle that was untreated prior to normal EM preparative procedures was compared to cuticle subjected to lipid solvents, high temperature, and concentrated alkali. Surface morphology of untreated intersegmental cuticle was also examined. The epicuticle is composed of four sublayers: outer membrane, outer epicuticle, cuticulin, and the dense homogeneous layer. Lipid solvents did not significantly alter the morphology of any of these layers or the contents of the wax canals that penetrate the cuticulin layer even though the solvents effectively remove lipids from the epicuticle for chemical analysis. The surface of the sclerite cuticle contains amorphous particles, crystalline projections, and scattered openings to dermal gland ducts. Perforations that correspond to the opening of wax canals were faintly visible after extraction of surface waxes and clearly visible after KOH treatment. No openings to dermal gland ducts or wax canals were observed in untreated intersegmental cuticle. However, wax canals are likely obscured by surface waxes similar to those present in sclerite cuticle.", "contents": "Fine structure of the epicuticle of the desert scorpion, Hadrurus arizonensis, with reference to location of lipids. The surface and transverse sections of the epicuticle of the desert scorpion, Hadrurus arizonensis, were examined by scanning and transmission electron microscopy, respectively. Sclerite cuticle that was untreated prior to normal EM preparative procedures was compared to cuticle subjected to lipid solvents, high temperature, and concentrated alkali. Surface morphology of untreated intersegmental cuticle was also examined. The epicuticle is composed of four sublayers: outer membrane, outer epicuticle, cuticulin, and the dense homogeneous layer. Lipid solvents did not significantly alter the morphology of any of these layers or the contents of the wax canals that penetrate the cuticulin layer even though the solvents effectively remove lipids from the epicuticle for chemical analysis. The surface of the sclerite cuticle contains amorphous particles, crystalline projections, and scattered openings to dermal gland ducts. Perforations that correspond to the opening of wax canals were faintly visible after extraction of surface waxes and clearly visible after KOH treatment. No openings to dermal gland ducts or wax canals were observed in untreated intersegmental cuticle. However, wax canals are likely obscured by surface waxes similar to those present in sclerite cuticle."}
{"id": "PMID:473162", "title": "Apolysis and the turnover of plasma membrane plaques during cuticle formation in an insect.", "content": "The apical plasma membranes of Calpodes epidermal cells have small fattened areas or plaques with an extra density upon their cytoplasmic face. The plaques are typically at the tips of microvilli. The are present during the deposition of fibrous cuticle and the cuticulin layer. Since the plaques are close (less than 15nm) to the sites where these kinds of cuticle first appear, they are presumed to have a role in their synthesis and/or deposition and orientation. When fifth stage larval cuticle deposition ceases prior to pupation, the plaques are lost as the area of the apical plasma membrane is reduced. The plaques pass from the surface into pinocytosis vesicles and multivesicular bodies where they are presumably digested. The loss of plaques occurs as the blood level of moulting hormone reaches a peak at the critical period after which the prothoracic glands are no longer needed for pupation. Apolysis or separation of the epidermis from the old cuticle is the stage when plaques are absent, the old ones have been lost but the new ones have yet to form. After the critical period, the epidermis prepared for pupation with a phase of elevated RNA synthesis at the end of which plaques and microvilli reform in time to secrete the new cuticulin layer and later the fibrous cuticle of the pharate pupa. There is a new generation of plaques for each moult and succeeding intermoult and each generation is involved in two kinds of cuticle deposition before involution and redifferentiation.", "contents": "Apolysis and the turnover of plasma membrane plaques during cuticle formation in an insect. The apical plasma membranes of Calpodes epidermal cells have small fattened areas or plaques with an extra density upon their cytoplasmic face. The plaques are typically at the tips of microvilli. The are present during the deposition of fibrous cuticle and the cuticulin layer. Since the plaques are close (less than 15nm) to the sites where these kinds of cuticle first appear, they are presumed to have a role in their synthesis and/or deposition and orientation. When fifth stage larval cuticle deposition ceases prior to pupation, the plaques are lost as the area of the apical plasma membrane is reduced. The plaques pass from the surface into pinocytosis vesicles and multivesicular bodies where they are presumably digested. The loss of plaques occurs as the blood level of moulting hormone reaches a peak at the critical period after which the prothoracic glands are no longer needed for pupation. Apolysis or separation of the epidermis from the old cuticle is the stage when plaques are absent, the old ones have been lost but the new ones have yet to form. After the critical period, the epidermis prepared for pupation with a phase of elevated RNA synthesis at the end of which plaques and microvilli reform in time to secrete the new cuticulin layer and later the fibrous cuticle of the pharate pupa. There is a new generation of plaques for each moult and succeeding intermoult and each generation is involved in two kinds of cuticle deposition before involution and redifferentiation."}
{"id": "PMID:473163", "title": "Changes in tight junctions of rat intestinal crypt cells associated with changes in their mitotic activity.", "content": "The freeze-fracture appearance of tight junctions between rat duodenal crypt cells was studied in normal, mitotically suppressed, and mitotically enhanced animals. In normal animals crypt cell tight junctions present a pleomorphic appearance. The population includes junctions resembling postmitotic junctions of the intestinal villus, junctions composed largely or completely of particle chains, and regions at the cell apex in which junctions are absent for 3-4 micron distances laterally. Mitotic suppression by inhibition of DNA synthesis with cytosine arabinoside results in the disappearance of pleomorphism and crypt tight junctions progressively come to resemble those of the intestinal villus. With recovery from the drug and further synchronization with Colcemid, the crypt cells undergo a mitotic burst, and all varieties of unusual junctional configurations are observed with increased frequency.", "contents": "Changes in tight junctions of rat intestinal crypt cells associated with changes in their mitotic activity. The freeze-fracture appearance of tight junctions between rat duodenal crypt cells was studied in normal, mitotically suppressed, and mitotically enhanced animals. In normal animals crypt cell tight junctions present a pleomorphic appearance. The population includes junctions resembling postmitotic junctions of the intestinal villus, junctions composed largely or completely of particle chains, and regions at the cell apex in which junctions are absent for 3-4 micron distances laterally. Mitotic suppression by inhibition of DNA synthesis with cytosine arabinoside results in the disappearance of pleomorphism and crypt tight junctions progressively come to resemble those of the intestinal villus. With recovery from the drug and further synchronization with Colcemid, the crypt cells undergo a mitotic burst, and all varieties of unusual junctional configurations are observed with increased frequency."}
{"id": "PMID:473164", "title": "Cellular junctions of the midgut in the centipede (Scutigerella pagesi) as revealed by lanthanum tracer and freeze-fracture technique.", "content": "In the midgut of a Myriapoda continuous junctions and gap junctions are described for the first time. Continuous junctions form a belt around the upper two-thirds of each cell. In the intercellular space long and smooth septa are clustered in sinuous strands and intramembrane particles appear on the PF. In the gap junctions the intramembrane particles are located on the EF.", "contents": "Cellular junctions of the midgut in the centipede (Scutigerella pagesi) as revealed by lanthanum tracer and freeze-fracture technique. In the midgut of a Myriapoda continuous junctions and gap junctions are described for the first time. Continuous junctions form a belt around the upper two-thirds of each cell. In the intercellular space long and smooth septa are clustered in sinuous strands and intramembrane particles appear on the PF. In the gap junctions the intramembrane particles are located on the EF."}
{"id": "PMID:473165", "title": "The organization and isolating function of insect rectal sheath cells: a freeze-fracture study.", "content": "In termites and roaches the well-defined rectal papillae each comprise a layer of columnar principal cells specialized for active transport and a layer of basal cells. The whole cell group is entirely surrounded by several series of flattened 'sheath cells' (formerly termed 'junctional cells') which abut onto the basal components of the papilla. The sheath cells secrete a specialized sclerified cuticle which forms the framework of the papilla. Their regularly pleated apical membrane is closely apposed to the cuticle and contains parallel and closely spaced rows of intramembranous particles. at this level, no subcuticular space is present and hence the space associated with the apical surface of the principal cells is defined as an isolated compartment. Typical septate junctions are present between the sheath and basal cells; however those linking adjacent sheath cells are structurally unusual: they extend to the basal surface rather than being restricted to the apical zone, are frequently interrupted and in replicas are represented by relatively short and irregularly oriented particle rows. Moreover, lateral sheath cell contacts display two further peculiarities: absence of an apical desmosomal ring and paucity of gap junctions. Structural observations suggest that the sheath cells isolate the principal cells from communication with the hemolymph, consequently enhancing their efficiency in water and ionic regulation. Comparable cells have been described in a number of insects, but the 'isolation' system presents varying degrees of complexity, for which an evolutionary scheme is proposed.", "contents": "The organization and isolating function of insect rectal sheath cells: a freeze-fracture study. In termites and roaches the well-defined rectal papillae each comprise a layer of columnar principal cells specialized for active transport and a layer of basal cells. The whole cell group is entirely surrounded by several series of flattened 'sheath cells' (formerly termed 'junctional cells') which abut onto the basal components of the papilla. The sheath cells secrete a specialized sclerified cuticle which forms the framework of the papilla. Their regularly pleated apical membrane is closely apposed to the cuticle and contains parallel and closely spaced rows of intramembranous particles. at this level, no subcuticular space is present and hence the space associated with the apical surface of the principal cells is defined as an isolated compartment. Typical septate junctions are present between the sheath and basal cells; however those linking adjacent sheath cells are structurally unusual: they extend to the basal surface rather than being restricted to the apical zone, are frequently interrupted and in replicas are represented by relatively short and irregularly oriented particle rows. Moreover, lateral sheath cell contacts display two further peculiarities: absence of an apical desmosomal ring and paucity of gap junctions. Structural observations suggest that the sheath cells isolate the principal cells from communication with the hemolymph, consequently enhancing their efficiency in water and ionic regulation. Comparable cells have been described in a number of insects, but the 'isolation' system presents varying degrees of complexity, for which an evolutionary scheme is proposed."}
{"id": "PMID:473167", "title": "Vitellogenesis stimulated by thoracic ganglion implants into destalked immature spider crabs, Libinia emarginata.", "content": "Since destalked immature female Libinia emarginata fail to molt to maturity (Hinsch, 1972) and implants of thoracic ganglion of adult females into young females of Potamon dehaani caused a doubling of ovarian weight and differentiation of yolk granules (Otsu, 1963), the effects of the thoracic ganglion implantation on sexual maturation in Libinia was investigated. Destalked immature Libinia with a maximum carapace length of 4-5 cm received two implants of adult female thoracic ganglion at 10-day intervals. All of the surviving experimental animals and destalked controls molted to immaturity. Most of the experimental animals failed to complete a successful molt and few survived longer than 3-4 days. The experimental animals that survived for over a week showed signs of yolk deposition not observed in the destalked controls. Eggs in several stages of vitellogenesis were scattered throughout the gonad in the animal showing the most pronounced effect. This was not observed in any of the destalked controls.", "contents": "Vitellogenesis stimulated by thoracic ganglion implants into destalked immature spider crabs, Libinia emarginata. Since destalked immature female Libinia emarginata fail to molt to maturity (Hinsch, 1972) and implants of thoracic ganglion of adult females into young females of Potamon dehaani caused a doubling of ovarian weight and differentiation of yolk granules (Otsu, 1963), the effects of the thoracic ganglion implantation on sexual maturation in Libinia was investigated. Destalked immature Libinia with a maximum carapace length of 4-5 cm received two implants of adult female thoracic ganglion at 10-day intervals. All of the surviving experimental animals and destalked controls molted to immaturity. Most of the experimental animals failed to complete a successful molt and few survived longer than 3-4 days. The experimental animals that survived for over a week showed signs of yolk deposition not observed in the destalked controls. Eggs in several stages of vitellogenesis were scattered throughout the gonad in the animal showing the most pronounced effect. This was not observed in any of the destalked controls."}
{"id": "PMID:473168", "title": "Occluding-like junctions at mesaxons of central myelin in Anolis carolinensis are not 'tight'. A freeze-fracture-protein tracer analysis.", "content": "The junctional complexes of the myelin sheath of central nervous system axons in the American chameleon, Anolis carolinensis, exhibit an intramembrane ridge and groove construction in freeze-fracture replicas that has usually been interpreted in other organisms as evidence for an occluding or tight intercellular junction. Close examination of PF fracture face ridges, however, shows them to be made up of discontinuous rows of particles of variable length separated by frequent gaps of non-uniform width. Introduction of horseradish peroxidase into the intercellular milieu of the lizard central nervous system is followed by appearance of this protein in interlamellar spaces of the myelin sheath and in the intercellular spaces containing focal membrane fusions that correspond precisely in position and center-to-center spacing to the ridges and grooves in platinum replicas of the same tissue. Since the junctional ridges on PF fracture faces in these mesaxonal junctional complexes are conspicuously discontinuous and since the areas within the myelin sheath where these junctional complexes are located inner and outer mesaxons) are readily permeated by exogenous protein tracer, it is concluded that the junctional complexes of central myelin mesaxons, heretofore incorrectly interpreted as functionally tight, are actually very leaky and probably contribute only to the structural stability of the myelin sheath architecture.", "contents": "Occluding-like junctions at mesaxons of central myelin in Anolis carolinensis are not 'tight'. A freeze-fracture-protein tracer analysis. The junctional complexes of the myelin sheath of central nervous system axons in the American chameleon, Anolis carolinensis, exhibit an intramembrane ridge and groove construction in freeze-fracture replicas that has usually been interpreted in other organisms as evidence for an occluding or tight intercellular junction. Close examination of PF fracture face ridges, however, shows them to be made up of discontinuous rows of particles of variable length separated by frequent gaps of non-uniform width. Introduction of horseradish peroxidase into the intercellular milieu of the lizard central nervous system is followed by appearance of this protein in interlamellar spaces of the myelin sheath and in the intercellular spaces containing focal membrane fusions that correspond precisely in position and center-to-center spacing to the ridges and grooves in platinum replicas of the same tissue. Since the junctional ridges on PF fracture faces in these mesaxonal junctional complexes are conspicuously discontinuous and since the areas within the myelin sheath where these junctional complexes are located inner and outer mesaxons) are readily permeated by exogenous protein tracer, it is concluded that the junctional complexes of central myelin mesaxons, heretofore incorrectly interpreted as functionally tight, are actually very leaky and probably contribute only to the structural stability of the myelin sheath architecture."}
{"id": "PMID:473169", "title": "Octopus microvasculature: permeability to ferritin and carbon.", "content": "The permeability of Octopus microvasculature was investigated by intravascular injection of carbon and ferritin. Vessels were tight to carbon while ferritin penetrated the pericyte junction, and was found extravascularly 1-2 min after its introduction. Vesicles occurred rarely in pericytes; fenestrae were absent. The discontinuous endothelial layer did not consitute a permeability barrier. The basement membrane, although retarding the movement of ferritin, was permeable to it; carbon did not penetrate the basement membrane. Evidence indicated that ferritin, and thus similarly sized and smaller water soluble materials, traverse the pericyte junction as a result of bulk fluid flow. Comparisons are made with the convective (or junctional) and slower, diffusive (or vesicular) passage of materials known to occur across the endothelium of continuous capillaries in mammals. Previous macrophysiological determinations concerning the permeability of Octopus vessels are questioned in view of these findings. Possible reasons for some major structural differences in the microcirculatory systems of cephalopods and vertebrates are briefly discussed.", "contents": "Octopus microvasculature: permeability to ferritin and carbon. The permeability of Octopus microvasculature was investigated by intravascular injection of carbon and ferritin. Vessels were tight to carbon while ferritin penetrated the pericyte junction, and was found extravascularly 1-2 min after its introduction. Vesicles occurred rarely in pericytes; fenestrae were absent. The discontinuous endothelial layer did not consitute a permeability barrier. The basement membrane, although retarding the movement of ferritin, was permeable to it; carbon did not penetrate the basement membrane. Evidence indicated that ferritin, and thus similarly sized and smaller water soluble materials, traverse the pericyte junction as a result of bulk fluid flow. Comparisons are made with the convective (or junctional) and slower, diffusive (or vesicular) passage of materials known to occur across the endothelium of continuous capillaries in mammals. Previous macrophysiological determinations concerning the permeability of Octopus vessels are questioned in view of these findings. Possible reasons for some major structural differences in the microcirculatory systems of cephalopods and vertebrates are briefly discussed."}
{"id": "PMID:473221", "title": "Post natal side effects in rats induced by hypolipidaemic treatment during pregnancy.", "content": "Maternal, foetal and peri- and post-natal toxicity studies of a hypolipidaemic agent, 2-[p-(2,2-dichlorocyclopropyl)phenoxy]-2-methyl propionic acid (WIN 35833), were performed on different animal species during animal evaluation of this new drug. In teratology studies in rats, mice and rabbits, no adverse effects were detected following drug exposure during the organogenesis period. In peri-natal studies, a neonatal abnormality related to treatment just before term was shown in rats. This modification, previously observed with other hypolipidaemic drugs, seems to be species specific and characteristic of compounds in the aryloxyalkanoic acid series.", "contents": "Post natal side effects in rats induced by hypolipidaemic treatment during pregnancy. Maternal, foetal and peri- and post-natal toxicity studies of a hypolipidaemic agent, 2-[p-(2,2-dichlorocyclopropyl)phenoxy]-2-methyl propionic acid (WIN 35833), were performed on different animal species during animal evaluation of this new drug. In teratology studies in rats, mice and rabbits, no adverse effects were detected following drug exposure during the organogenesis period. In peri-natal studies, a neonatal abnormality related to treatment just before term was shown in rats. This modification, previously observed with other hypolipidaemic drugs, seems to be species specific and characteristic of compounds in the aryloxyalkanoic acid series."}
{"id": "PMID:473222", "title": "Dose-related alterations in growth and mineral disposition by chronic oral cadmium administration in the male rat.", "content": "The effect of cadmium (1, 10, or 100 ppm) administered to male rats in drinking water for 13 weeks on body weight and mineral disposition (Cd, Mg, P and Zn) in several body tissues was examined. Most alterations observed in these parameters occurred only at the 100 ppm dose of Cd. Terminal body weight was decreased by 10% in rats ingesting Cd at 100 ppm resulting from decreased food intake since weight gain/food consumption ratio was the same for all treatment groups. In serum, cadmium ingestion resulted in an inhibition of alkaline phosphatase activity at all concentrations and phosphorous was elevated only in animals receiving 100 ppm Cd. No changes were observed in Ca in urea. In bone, Cd decreased zinc content, increased Ca content, but did not influence bone ash, Mg or P and roentgenographic examination revealed no bone abnormalities. In both liver and kidney, cadmium ingestion did not influence intestinal absorption of Ca, Mg, P, or Zn or the renal excretion of Ca, P, or urea. The results of this study indicate that alterations in body weight and tissue mineral disposition resulting from chronic Cd ingestion are dose-related.", "contents": "Dose-related alterations in growth and mineral disposition by chronic oral cadmium administration in the male rat. The effect of cadmium (1, 10, or 100 ppm) administered to male rats in drinking water for 13 weeks on body weight and mineral disposition (Cd, Mg, P and Zn) in several body tissues was examined. Most alterations observed in these parameters occurred only at the 100 ppm dose of Cd. Terminal body weight was decreased by 10% in rats ingesting Cd at 100 ppm resulting from decreased food intake since weight gain/food consumption ratio was the same for all treatment groups. In serum, cadmium ingestion resulted in an inhibition of alkaline phosphatase activity at all concentrations and phosphorous was elevated only in animals receiving 100 ppm Cd. No changes were observed in Ca in urea. In bone, Cd decreased zinc content, increased Ca content, but did not influence bone ash, Mg or P and roentgenographic examination revealed no bone abnormalities. In both liver and kidney, cadmium ingestion did not influence intestinal absorption of Ca, Mg, P, or Zn or the renal excretion of Ca, P, or urea. The results of this study indicate that alterations in body weight and tissue mineral disposition resulting from chronic Cd ingestion are dose-related."}
{"id": "PMID:473223", "title": "Effects of mercuric chloride on the resting membrane potentials of blue crab (Callinectes sapidus) muscle fibers.", "content": "The effects of concentrations of mercuric chloride ranging from 3.68 to 368 microM (1--100 ppm) on the resting membrane potentials of muscle fibers in the extensor muscle of the carpopodite in walking legs of adults blue crabs were investigated in vitro. Glass micropipettes filled with saturated solutions of potassium chloride and conventional electronic equipment were used. Resting membrane potentials in uncontaminated artificial sea water averaged -78 mV +/- 4 mV (S.E.M.). Mercuric chloride added to the control bath at 3.68 or 9.2 microM did not affect the resting membrane potentials. At 18.4 microM threshold effects were obtained in 50% of the preparations. At concentrations ranging from 25.7 to 368 microM progressive deterioration of membrane polarization occurred as the mercury concentration was increased. This effect was irreversible despite multiple washes with normal sea water. We conclude that mercuric chloride has very powerful toxic effects at the peripheral motor effector elements in blue crabs. These results do not, of course, rule out more central effects on enzymatic systems or neuronal networks.", "contents": "Effects of mercuric chloride on the resting membrane potentials of blue crab (Callinectes sapidus) muscle fibers. The effects of concentrations of mercuric chloride ranging from 3.68 to 368 microM (1--100 ppm) on the resting membrane potentials of muscle fibers in the extensor muscle of the carpopodite in walking legs of adults blue crabs were investigated in vitro. Glass micropipettes filled with saturated solutions of potassium chloride and conventional electronic equipment were used. Resting membrane potentials in uncontaminated artificial sea water averaged -78 mV +/- 4 mV (S.E.M.). Mercuric chloride added to the control bath at 3.68 or 9.2 microM did not affect the resting membrane potentials. At 18.4 microM threshold effects were obtained in 50% of the preparations. At concentrations ranging from 25.7 to 368 microM progressive deterioration of membrane polarization occurred as the mercury concentration was increased. This effect was irreversible despite multiple washes with normal sea water. We conclude that mercuric chloride has very powerful toxic effects at the peripheral motor effector elements in blue crabs. These results do not, of course, rule out more central effects on enzymatic systems or neuronal networks."}
{"id": "PMID:473224", "title": "Chromium-induced hyperglycemia in the rat.", "content": "The acute administration of chromium chloride (Cr) produces hyperglycemia in rats. Adrenalectomy prevents the hyperglycemic effect but not the glucose intolerance. Subacute exposure of rats to Cr for 7--23 days does not change the resting blood glucose nor is there an acute or subacute effect on tolerance to glucose load. Administration of Cr does not influence acute insulin hypoglycemia. Thus contrary to Cr deficient animal, at the concentrations tested, Cr appears to be a hyperglycmiec agent to rats with minimal subacute effects.", "contents": "Chromium-induced hyperglycemia in the rat. The acute administration of chromium chloride (Cr) produces hyperglycemia in rats. Adrenalectomy prevents the hyperglycemic effect but not the glucose intolerance. Subacute exposure of rats to Cr for 7--23 days does not change the resting blood glucose nor is there an acute or subacute effect on tolerance to glucose load. Administration of Cr does not influence acute insulin hypoglycemia. Thus contrary to Cr deficient animal, at the concentrations tested, Cr appears to be a hyperglycmiec agent to rats with minimal subacute effects."}
{"id": "PMID:473225", "title": "In vivo and in vitro changes in renal function caused by ochratoxin A in the rat.", "content": "Ochratoxin A has been suggested to be an important, environmentally prominent nephrotoxin. Possible involvement in both human and animal disease states has been indicated. This study was designed to examine under controlled laboratory conditions, the nephrotoxic potential of ochratoxin A in the rat. Daily administration of ochratoxin A in doses of 0.75 and 2.0 mg/kg caused persistent urinary hypoosmolality coupled with excessive glucose and protein excretion. In addition the renal slice transport of several organic compounds also was altered. Renal slice tissue electrolytes and water were unaffected by ochratoxin A pretreatment. Direct addition of ochratoxin A to fresh renal cortex slices also depressed transport of some organic compounds. Although rats pretreated with ochratoxin A lost large amounts of body weight, the weight loss alone did not account totally for the changes in renal function. These data indicate that ochratoxin A has the capability of producing alterations in renal function suggestive of nephrotoxicity. However, the pattern of response is different than that observed with citrinin.", "contents": "In vivo and in vitro changes in renal function caused by ochratoxin A in the rat. Ochratoxin A has been suggested to be an important, environmentally prominent nephrotoxin. Possible involvement in both human and animal disease states has been indicated. This study was designed to examine under controlled laboratory conditions, the nephrotoxic potential of ochratoxin A in the rat. Daily administration of ochratoxin A in doses of 0.75 and 2.0 mg/kg caused persistent urinary hypoosmolality coupled with excessive glucose and protein excretion. In addition the renal slice transport of several organic compounds also was altered. Renal slice tissue electrolytes and water were unaffected by ochratoxin A pretreatment. Direct addition of ochratoxin A to fresh renal cortex slices also depressed transport of some organic compounds. Although rats pretreated with ochratoxin A lost large amounts of body weight, the weight loss alone did not account totally for the changes in renal function. These data indicate that ochratoxin A has the capability of producing alterations in renal function suggestive of nephrotoxicity. However, the pattern of response is different than that observed with citrinin."}
{"id": "PMID:473226", "title": "The influence of ethanol treatment of cytogenetic effects in bone marrow cells of Chinese hamsters by cyclophosphamide, aflatoxin B1 and patulin.", "content": "Chromosomes were investigated from the bone marrow of Chinese hamsters which received 10% (v/v) ethanol as the only liquid supply for a period of 9 weeks. At the end of the ethanol drinking period 1 group of animals received 2 oral doses of 80 mg/kg cyclophosphamide (CP), a second group received 2 oral doses of 25 mg/kg aflatoxin B1 (AFB1), a third group 2 oral doses of 20 mg/kg patulin (PA). The 2  applications were separated by 24 h. The intake of ethanol had no effect on the bone marrow chromosomes, and had no potentiating effect on CP induced aberrations. 9 weeks consumption of 10% (v/v) ethanol revealed likewise no influence on the frequencies of chromosomal aberrations induced by the indirect mutagen AFB1. However, the rate of chromosomal aberrations induced by the direct mutagen PA was clearly suppressed in ethanol drinking animals.", "contents": "The influence of ethanol treatment of cytogenetic effects in bone marrow cells of Chinese hamsters by cyclophosphamide, aflatoxin B1 and patulin. Chromosomes were investigated from the bone marrow of Chinese hamsters which received 10% (v/v) ethanol as the only liquid supply for a period of 9 weeks. At the end of the ethanol drinking period 1 group of animals received 2 oral doses of 80 mg/kg cyclophosphamide (CP), a second group received 2 oral doses of 25 mg/kg aflatoxin B1 (AFB1), a third group 2 oral doses of 20 mg/kg patulin (PA). The 2  applications were separated by 24 h. The intake of ethanol had no effect on the bone marrow chromosomes, and had no potentiating effect on CP induced aberrations. 9 weeks consumption of 10% (v/v) ethanol revealed likewise no influence on the frequencies of chromosomal aberrations induced by the indirect mutagen AFB1. However, the rate of chromosomal aberrations induced by the direct mutagen PA was clearly suppressed in ethanol drinking animals."}
{"id": "PMID:473227", "title": "Absorption, distribution and metabolism of propyl anthranilate.", "content": "Analysis of the gut contents of rats killed at intervals after dosage with propyl anthranilate or anthranilic acid suggested that both acid and ester were absorbed rapidly; the chief site of absorption was the stomach. Not more than a trace of the ester was hydrolysed in the stomach but of the dosed ester detected in the small intestine (less than 7%) a considerable proportion, increasing with time after dosing was present as anthranilic acid. Measurement of the level of radioactivity in the blood after administration of 14C-labelled propan-1-ol, propyl anthranilate or anthranilic acid showed that the alcohol was absorbed more rapidly than either the ester or acid. Unchanged propyl anthranilate was readily detected in the blood of rats dosed with the ester and some unhydrolysed ester was excreted in the urine. The level of radioactivity of the organs of rats 2 h and 4 h after administration of the 14C-labelled compounds was measured. Velocity constants for the excretion of 14CO2 by rats dosed with 14C-labelled propyl anthranilate were significantly lower than those found for rats dosed with [14C]propan-1-ol indicating a limiting step in the metabolism of the propyl moiety of the ester which did not occur in the metabolism of propan-1-ol. The urinary metabolites of anthranilic acid excreted by rabbits and rats dosed with the acid were qualitatively the same as those excreted by these species after dosing with propyl anthanilate; some quantitative differences were however, observed.", "contents": "Absorption, distribution and metabolism of propyl anthranilate. Analysis of the gut contents of rats killed at intervals after dosage with propyl anthranilate or anthranilic acid suggested that both acid and ester were absorbed rapidly; the chief site of absorption was the stomach. Not more than a trace of the ester was hydrolysed in the stomach but of the dosed ester detected in the small intestine (less than 7%) a considerable proportion, increasing with time after dosing was present as anthranilic acid. Measurement of the level of radioactivity in the blood after administration of 14C-labelled propan-1-ol, propyl anthranilate or anthranilic acid showed that the alcohol was absorbed more rapidly than either the ester or acid. Unchanged propyl anthranilate was readily detected in the blood of rats dosed with the ester and some unhydrolysed ester was excreted in the urine. The level of radioactivity of the organs of rats 2 h and 4 h after administration of the 14C-labelled compounds was measured. Velocity constants for the excretion of 14CO2 by rats dosed with 14C-labelled propyl anthranilate were significantly lower than those found for rats dosed with [14C]propan-1-ol indicating a limiting step in the metabolism of the propyl moiety of the ester which did not occur in the metabolism of propan-1-ol. The urinary metabolites of anthranilic acid excreted by rabbits and rats dosed with the acid were qualitatively the same as those excreted by these species after dosing with propyl anthanilate; some quantitative differences were however, observed."}
{"id": "PMID:473228", "title": "Wholebody elimination of 75SeO2-3 and 203HgCl2 administered separately and simultaneously to mice.", "content": "A study of wholebody elimination of mercury (203HgCl2) and selenium (75SeO2-3) given separately or simultaneously in single doses by intraperitoneal injections to mice has been performed. The conclusion of this study can be summarized as follows: there seems to exist a homeostatic mechanism for selenium, but not for mercury; increased retention is observed for both elements when administered simultaneously. Maximum retention of 1 element is observed, when the other is given in excess or equimolar ratio to the other. Excess mercury provokes a pattern of selenium retention similar to that found in case of selenium deficiency. The wholebody molar ratio of mercury and selenium tends towards 1. When this happens, mercury as well as selenium are eliminated at a rate different from what is seen when the elements are administered separately. Selenium therefore seems to play a direct role in metabolism of inorganic mercury.", "contents": "Wholebody elimination of 75SeO2-3 and 203HgCl2 administered separately and simultaneously to mice. A study of wholebody elimination of mercury (203HgCl2) and selenium (75SeO2-3) given separately or simultaneously in single doses by intraperitoneal injections to mice has been performed. The conclusion of this study can be summarized as follows: there seems to exist a homeostatic mechanism for selenium, but not for mercury; increased retention is observed for both elements when administered simultaneously. Maximum retention of 1 element is observed, when the other is given in excess or equimolar ratio to the other. Excess mercury provokes a pattern of selenium retention similar to that found in case of selenium deficiency. The wholebody molar ratio of mercury and selenium tends towards 1. When this happens, mercury as well as selenium are eliminated at a rate different from what is seen when the elements are administered separately. Selenium therefore seems to play a direct role in metabolism of inorganic mercury."}
{"id": "PMID:473229", "title": "Effects of methylene chloride, trichloroethane, trichloroethylene, tetrachloroethylene and toluene on the development of chick embryos.", "content": "Toluene and 5 aliphatic chlorinated hydrocarbons of wide industrial use were injected into the air space of fertilized chicken eggs at 2, 3 and 6 days of incubation. The embryotoxicity was evaluated as survival and death incidences after 14 days of incubation, and also the weights and lengths of the embryos were recorded. The approximate LD50 value for trichloroethylene and trichloroethanes varied between 50 and 100 mumol/egg while for toluene, tetrachloroethylene and methylene chloride it was over 100 mumol/egg. Macroscopic malformations of various kinds were produced with doses of 5-100 mumol/egg. The teratogenic potential of the tested compounds decreased in the following order: 1,1,1-trichloroethane greater than trichloroethylene greater than methylene chloride, tetrachloroethylene, 1,1,2-trichloroethane greater than toluene greater than olive oil control.", "contents": "Effects of methylene chloride, trichloroethane, trichloroethylene, tetrachloroethylene and toluene on the development of chick embryos. Toluene and 5 aliphatic chlorinated hydrocarbons of wide industrial use were injected into the air space of fertilized chicken eggs at 2, 3 and 6 days of incubation. The embryotoxicity was evaluated as survival and death incidences after 14 days of incubation, and also the weights and lengths of the embryos were recorded. The approximate LD50 value for trichloroethylene and trichloroethanes varied between 50 and 100 mumol/egg while for toluene, tetrachloroethylene and methylene chloride it was over 100 mumol/egg. Macroscopic malformations of various kinds were produced with doses of 5-100 mumol/egg. The teratogenic potential of the tested compounds decreased in the following order: 1,1,1-trichloroethane greater than trichloroethylene greater than methylene chloride, tetrachloroethylene, 1,1,2-trichloroethane greater than toluene greater than olive oil control."}
{"id": "PMID:473230", "title": "Studies on the mechanism of paracetamol-induced protection against paracetamol hepatotoxicity.", "content": "In rats, 3 days treatment with paracetamol (1 oral dose of 1 g/kg daily) produced a complete protection against the hepatotoxic actions of a further dose of paracetamol as documented by determination of serum enzyme activities (glutamic-oxaloacetic transaminase, (GOT), glutamic-pyruvic transaminase (GPT), sorbitol dehydrogenase (SDH), bromsulphthalein retention and histological investigations. Subacute paracetamol treatment decreased liver glutathione levels by 46%, liver microsomal cytochrome P-450 content by 23%, hepatic hydroxylation of aniline by 29% and hepatic demethylation of aminopyrine by 46%. It afforded also some protection against the hepatotoxic actions of carbon tetrachloride, bromobenzene and thioacetamide, but did not influence the antiphlogistic activity of paracetamol (carrageenan paw edema test). Plasma and liver concentrations of free paracetamol after oral administration of 1 g/kg paracetamol were somewhat higher in the subacutely paracetamol-pretreated rats than in the non-pretreated control animals whereas no differences in the concentrations of conjugated paracetamol were found between the 2 groups. Pretreatment with paracetamol did not influence the urinary excretion of free paracetamol but caused some shift in the urinary excretion of paracetamol conjugates: pretreated rats excreted 23% less of the paracetamol glucuronide and sulfate and 33% more of the paracetamol mercapturate than the control animals. A depression of the microsomal mixed-function oxidase activity is presumed to be the main cause of the paracetamol-induced protection against paracetamol hepatotoxicity.", "contents": "Studies on the mechanism of paracetamol-induced protection against paracetamol hepatotoxicity. In rats, 3 days treatment with paracetamol (1 oral dose of 1 g/kg daily) produced a complete protection against the hepatotoxic actions of a further dose of paracetamol as documented by determination of serum enzyme activities (glutamic-oxaloacetic transaminase, (GOT), glutamic-pyruvic transaminase (GPT), sorbitol dehydrogenase (SDH), bromsulphthalein retention and histological investigations. Subacute paracetamol treatment decreased liver glutathione levels by 46%, liver microsomal cytochrome P-450 content by 23%, hepatic hydroxylation of aniline by 29% and hepatic demethylation of aminopyrine by 46%. It afforded also some protection against the hepatotoxic actions of carbon tetrachloride, bromobenzene and thioacetamide, but did not influence the antiphlogistic activity of paracetamol (carrageenan paw edema test). Plasma and liver concentrations of free paracetamol after oral administration of 1 g/kg paracetamol were somewhat higher in the subacutely paracetamol-pretreated rats than in the non-pretreated control animals whereas no differences in the concentrations of conjugated paracetamol were found between the 2 groups. Pretreatment with paracetamol did not influence the urinary excretion of free paracetamol but caused some shift in the urinary excretion of paracetamol conjugates: pretreated rats excreted 23% less of the paracetamol glucuronide and sulfate and 33% more of the paracetamol mercapturate than the control animals. A depression of the microsomal mixed-function oxidase activity is presumed to be the main cause of the paracetamol-induced protection against paracetamol hepatotoxicity."}
{"id": "PMID:473231", "title": "Effect of methyl substitution on the irritancy of dibenz[b,f]1,4-oxazepine(CR).", "content": "The irritant properties of 3 methylated derivatives of dibenz[b,f]1,4-oxazepine (CR) have been investigated, using the guinea-pig blepharospasm test and isolated cat eye preparations, and compared with the irritancy of the unsubstituted CR molecule. In both tests, the 11-methyl derivative was non-irritant whereas 3 Me-CR and 9 Me-CR retained some irritancy although both were less potent than CR itself. The possible significance of these results are discussed in relation to earlier biochemical studies.", "contents": "Effect of methyl substitution on the irritancy of dibenz[b,f]1,4-oxazepine(CR). The irritant properties of 3 methylated derivatives of dibenz[b,f]1,4-oxazepine (CR) have been investigated, using the guinea-pig blepharospasm test and isolated cat eye preparations, and compared with the irritancy of the unsubstituted CR molecule. In both tests, the 11-methyl derivative was non-irritant whereas 3 Me-CR and 9 Me-CR retained some irritancy although both were less potent than CR itself. The possible significance of these results are discussed in relation to earlier biochemical studies."}
{"id": "PMID:473232", "title": "Liver injury by the false morel poison gyromitrin.", "content": "After oral application of the mushroom poison gyromitrin a time and dose dependent decrease of cytochrome P-450 was found in rat liver microsomes. The maximal decrease to about 50-60% of the control (after 200 mg/kg, 80% of LD50) was observed 8-12h after application, a normalization after 48 h. The inhibition of cytochrome P-450 mediated metabolism of aminopyrine and p-nitroanisole corresponds to the decrease of cytochrome P-450. The specific activity of cytochrome P-450 remains unchanged while that of cytochrome P-448 is decreased as shown by means of the metabolism of ethoxycoumarin or ethoxyresorufin. Comparable results were obtained after application of N-methyl-N-formylhydrazine (MFH) which is formed from gyromitrin rapidly by hydrolysis. An attack on the endoplasmatic membrane with a stimulation of lipid peroxidation is discussed.", "contents": "Liver injury by the false morel poison gyromitrin. After oral application of the mushroom poison gyromitrin a time and dose dependent decrease of cytochrome P-450 was found in rat liver microsomes. The maximal decrease to about 50-60% of the control (after 200 mg/kg, 80% of LD50) was observed 8-12h after application, a normalization after 48 h. The inhibition of cytochrome P-450 mediated metabolism of aminopyrine and p-nitroanisole corresponds to the decrease of cytochrome P-450. The specific activity of cytochrome P-450 remains unchanged while that of cytochrome P-448 is decreased as shown by means of the metabolism of ethoxycoumarin or ethoxyresorufin. Comparable results were obtained after application of N-methyl-N-formylhydrazine (MFH) which is formed from gyromitrin rapidly by hydrolysis. An attack on the endoplasmatic membrane with a stimulation of lipid peroxidation is discussed."}
{"id": "PMID:473233", "title": "Whole-body autoradiography of 63Ni in mice throughout gestation.", "content": "Whole-body autoradiography was used to study nickel uptake and retention in mice throughout gestation. After an intraperitoneal injection of 50 muCi 63NiCl2 into a 16-day-pregnant mouse, nickel appeared rapidly in connective tissues. Prominent sites of radioactivity 72 h after injection included the visceral yolk sac, lung, gastrointestinal tract and kidney. Nickel crossed the placental barriers throughout gestation, i.e. the visceral yolk sac during early gestation, the visceral yolk sac and chorioallantoic placenta during late gestation. A marked uptake of nickel was seen already in the 5- and 6-day embryo. Fetal accumulation of nickel took place up to 16 days gestation. Whereas nickel was distributed throughout the early embryo, distribution became more differentiated with increasing gestation, imitating that in the mother. Penetration of nickel into the mouse conceptus makes possible a similar ability in the human conceptus.", "contents": "Whole-body autoradiography of 63Ni in mice throughout gestation. Whole-body autoradiography was used to study nickel uptake and retention in mice throughout gestation. After an intraperitoneal injection of 50 muCi 63NiCl2 into a 16-day-pregnant mouse, nickel appeared rapidly in connective tissues. Prominent sites of radioactivity 72 h after injection included the visceral yolk sac, lung, gastrointestinal tract and kidney. Nickel crossed the placental barriers throughout gestation, i.e. the visceral yolk sac during early gestation, the visceral yolk sac and chorioallantoic placenta during late gestation. A marked uptake of nickel was seen already in the 5- and 6-day embryo. Fetal accumulation of nickel took place up to 16 days gestation. Whereas nickel was distributed throughout the early embryo, distribution became more differentiated with increasing gestation, imitating that in the mother. Penetration of nickel into the mouse conceptus makes possible a similar ability in the human conceptus."}
{"id": "PMID:473235", "title": "Testicular lesions of coprine and benzcoprine.", "content": "The effect on the testis of the disulfiram-like compounds benzcoprine (N-[1-ethoxycyclopropyl] benzamide) and coprine (N5-[1-hydroxycyclopropyl]-L-glutamine) was studied in rats and dogs. Severe degeneration of the seminiferous epithelium was induced in rats by subacute oral administration of each compound. 60 days after termination of treatment with benzcoprine most seminiferous tubules contained only occasional spermatogonia and the testicular weight was markedly decreased. The blood-testis barrier was unaffected in the benzcoprine-treated rats as judged by a lanthanum tracer technique. In dogs, oral administration of benzcoprine for 1 month caused impaired spermatogenesis, degeneration of germ cells and a decrease in the testicular weight. The results indicate that both compounds act directly on the germ cells. The effect is similar to that of alkylating compounds. Other effects of benzcoprine and coprine (bone marrow depression, lymphocytopenia, positive Ames test in organisms sensitive to base-pair substitution) are well-known properties of alkylating agents. In conclusion benzcoprine and coprine were found to cause severe changes in the testis in rats and dogs, probably due to a direct effect on the germ cells.", "contents": "Testicular lesions of coprine and benzcoprine. The effect on the testis of the disulfiram-like compounds benzcoprine (N-[1-ethoxycyclopropyl] benzamide) and coprine (N5-[1-hydroxycyclopropyl]-L-glutamine) was studied in rats and dogs. Severe degeneration of the seminiferous epithelium was induced in rats by subacute oral administration of each compound. 60 days after termination of treatment with benzcoprine most seminiferous tubules contained only occasional spermatogonia and the testicular weight was markedly decreased. The blood-testis barrier was unaffected in the benzcoprine-treated rats as judged by a lanthanum tracer technique. In dogs, oral administration of benzcoprine for 1 month caused impaired spermatogenesis, degeneration of germ cells and a decrease in the testicular weight. The results indicate that both compounds act directly on the germ cells. The effect is similar to that of alkylating compounds. Other effects of benzcoprine and coprine (bone marrow depression, lymphocytopenia, positive Ames test in organisms sensitive to base-pair substitution) are well-known properties of alkylating agents. In conclusion benzcoprine and coprine were found to cause severe changes in the testis in rats and dogs, probably due to a direct effect on the germ cells."}
{"id": "PMID:473252", "title": "[ECG-changes following postoperative irradiation of patients with mammary carcinoma (author's transl)].", "content": "Systematic ECG-analyses and determinations of serum creatin-kinase were performed in 32 patients with mammary carcinoma of the left, prior to and immediately after postoperative radiation therapy. Treatment was done with a gammatron, during this, the average load to the anterior cardiac wall amounted to 3600 rd. Eleven cases exhibited coronary-negative T-waves in at least two chest leads after treatment, seven other cases had flat negative or isoelectric T-waves. Two patients additionally revealed prolongation of the relative QT-period by more than 115%. These changes are interpreted as a sign of radiation-induced myopericarditis. Pericardial effusions did not appear during the follow-up period, furthermore no modifications of creatin-kinase activity. Clinical injury to the patients developed in no case.", "contents": "[ECG-changes following postoperative irradiation of patients with mammary carcinoma (author's transl)]. Systematic ECG-analyses and determinations of serum creatin-kinase were performed in 32 patients with mammary carcinoma of the left, prior to and immediately after postoperative radiation therapy. Treatment was done with a gammatron, during this, the average load to the anterior cardiac wall amounted to 3600 rd. Eleven cases exhibited coronary-negative T-waves in at least two chest leads after treatment, seven other cases had flat negative or isoelectric T-waves. Two patients additionally revealed prolongation of the relative QT-period by more than 115%. These changes are interpreted as a sign of radiation-induced myopericarditis. Pericardial effusions did not appear during the follow-up period, furthermore no modifications of creatin-kinase activity. Clinical injury to the patients developed in no case."}
{"id": "PMID:473253", "title": "[Electroencephalographic findings in the late stage after CNS-irradiation for malignant neoplasms in children (author's transl)].", "content": "Of forty children having undergone two or more years ago an irradiation of the brain case with cobalt-60 delivering doses between 1800 and 2400 R TD (n = 20) or a dose above 3500 R TD (n = 20), as a matter of fact ten presented pathological EEG findings which, however, may be interpreted as genetically determined characteristics, or instead in connection with a recurrence of the tumor just manifesting itself, or else as the consequence of a residual tumor or of the surgery performed. Pathological EEG findings on the account of radiation therapy apparently are much less frequent than morphological changes of the brain after radiation therapy. It is not necessary to assume that the latter need always have an effect upon the function of the infantine brain.", "contents": "[Electroencephalographic findings in the late stage after CNS-irradiation for malignant neoplasms in children (author's transl)]. Of forty children having undergone two or more years ago an irradiation of the brain case with cobalt-60 delivering doses between 1800 and 2400 R TD (n = 20) or a dose above 3500 R TD (n = 20), as a matter of fact ten presented pathological EEG findings which, however, may be interpreted as genetically determined characteristics, or instead in connection with a recurrence of the tumor just manifesting itself, or else as the consequence of a residual tumor or of the surgery performed. Pathological EEG findings on the account of radiation therapy apparently are much less frequent than morphological changes of the brain after radiation therapy. It is not necessary to assume that the latter need always have an effect upon the function of the infantine brain."}
{"id": "PMID:473254", "title": "Roentgen treatment of calcified deposits. A special technique.", "content": "Radiation treatment of benign ailments has seriously been questioned in recent years. A decision for or against can only be based on a \"cost-benefit\" analysis. In the treatment of calcified deposits in the shoulder or hip region, the authors have used radiation qualities assuring optimal absorption in the deposits and having demonstrable beneficial effects. In order to minimize the \"cost\", the irradiation parameters have been considered with special care.", "contents": "Roentgen treatment of calcified deposits. A special technique. Radiation treatment of benign ailments has seriously been questioned in recent years. A decision for or against can only be based on a \"cost-benefit\" analysis. In the treatment of calcified deposits in the shoulder or hip region, the authors have used radiation qualities assuring optimal absorption in the deposits and having demonstrable beneficial effects. In order to minimize the \"cost\", the irradiation parameters have been considered with special care."}
{"id": "PMID:473255", "title": "[Study on the dose distribution of 8-MeV bremsstrahlung in mantle field techniques (author's transl)].", "content": "The dose distribution within the patient was studied with 8-MeV bremsstrahlung from a linear accelerator during mantle field irradiation using molded shielding blocks. Doses and dose distributions in the different layers of a modified Alderson phantom were measured by means of film dosimetry and related to the dose in the central ray beam at the middle of the body. Dose distribution within unshielded regions perpendicular to the central ray beam generally being relatively homogeneous, the highest relative doses, amounting to ca. 115%, are found in the region of the mandibular angle and in the supraclavicular region; the dose to superficial lymph nodes at the supraclavicular region reaches 100% of the dose in the central ray beam. As a cause for these important doses near the surface of the body are discussed the extension of mantle fields as well as the increased exit dose of the opposed field and the oblique incidence of radiation.", "contents": "[Study on the dose distribution of 8-MeV bremsstrahlung in mantle field techniques (author's transl)]. The dose distribution within the patient was studied with 8-MeV bremsstrahlung from a linear accelerator during mantle field irradiation using molded shielding blocks. Doses and dose distributions in the different layers of a modified Alderson phantom were measured by means of film dosimetry and related to the dose in the central ray beam at the middle of the body. Dose distribution within unshielded regions perpendicular to the central ray beam generally being relatively homogeneous, the highest relative doses, amounting to ca. 115%, are found in the region of the mandibular angle and in the supraclavicular region; the dose to superficial lymph nodes at the supraclavicular region reaches 100% of the dose in the central ray beam. As a cause for these important doses near the surface of the body are discussed the extension of mantle fields as well as the increased exit dose of the opposed field and the oblique incidence of radiation."}
{"id": "PMID:473256", "title": "On the measurement of low-voltage therapeutic X-ray units.", "content": "Skin exposure rate was measured on 22 low-voltage therapeutic X-ray generators at an FSD of 15 cm at each of four standard kV steps. The measurements of two consecutive years were compared and their variance was analyzed. The statistical fluctuation of these field measurements due to the ionization method was 2.58%. According to the present measurements the high inaccuracies of low-voltage X-ray therapy are mainly due to the lack of reproducibility of the X-ray generators and an insufficient calibration of the dosemeters.", "contents": "On the measurement of low-voltage therapeutic X-ray units. Skin exposure rate was measured on 22 low-voltage therapeutic X-ray generators at an FSD of 15 cm at each of four standard kV steps. The measurements of two consecutive years were compared and their variance was analyzed. The statistical fluctuation of these field measurements due to the ionization method was 2.58%. According to the present measurements the high inaccuracies of low-voltage X-ray therapy are mainly due to the lack of reproducibility of the X-ray generators and an insufficient calibration of the dosemeters."}
{"id": "PMID:473257", "title": "Radiation effects on the parotid gland of mammals. Part 2: Modifications of plasma and parotid amylase activity.", "content": "The early modifications of the activity of plasma and parotid amylase have been evaluated in rats after whole-body irradiation in different experimental conditions. The modifications observed in the rat are less evident than in humans. The results have shown an initial decrease and a subsequent increase appearing significant 74 hours after irradiation. This could be due to a direct action of ionizing radiation on the parotid glands. However we cannot exclude that the increase of alpha-amylase could be determined by the intestinal radiation syndrome. In fact, this syndrome leads to a reduced food uptake, and consequently alpha-amylase could accumulate in parotids.", "contents": "Radiation effects on the parotid gland of mammals. Part 2: Modifications of plasma and parotid amylase activity. The early modifications of the activity of plasma and parotid amylase have been evaluated in rats after whole-body irradiation in different experimental conditions. The modifications observed in the rat are less evident than in humans. The results have shown an initial decrease and a subsequent increase appearing significant 74 hours after irradiation. This could be due to a direct action of ionizing radiation on the parotid glands. However we cannot exclude that the increase of alpha-amylase could be determined by the intestinal radiation syndrome. In fact, this syndrome leads to a reduced food uptake, and consequently alpha-amylase could accumulate in parotids."}
{"id": "PMID:473258", "title": "The effect of preirradiation application of aspartic acid salts on hemopoietic recovery in X-irradiated mice.", "content": "The possibility of radioprotective action of K and Mg aspartate administered in tap water for ten days prior to X-irradiation was investigated in male mice of the strain C 57 Bl/10. In normal animals, thymus weight was found to be increased by 10-day treatment with K and Mg aspartate. The postirradiation regeneration of spleen weight and incorporation of radioactive iron into the spleen and femoral marrow following sublethal irradiation was favorably modified by the treatment used. Pretreatment of mice with K and Mg aspartate delays the onset of early deaths at irradiation with an absolutely lethal X-ray exposure and raises the percentage of surviving animals after nearly lethal exposures.", "contents": "The effect of preirradiation application of aspartic acid salts on hemopoietic recovery in X-irradiated mice. The possibility of radioprotective action of K and Mg aspartate administered in tap water for ten days prior to X-irradiation was investigated in male mice of the strain C 57 Bl/10. In normal animals, thymus weight was found to be increased by 10-day treatment with K and Mg aspartate. The postirradiation regeneration of spleen weight and incorporation of radioactive iron into the spleen and femoral marrow following sublethal irradiation was favorably modified by the treatment used. Pretreatment of mice with K and Mg aspartate delays the onset of early deaths at irradiation with an absolutely lethal X-ray exposure and raises the percentage of surviving animals after nearly lethal exposures."}
{"id": "PMID:473259", "title": "Investigation of a valuable biochemical indicator in radiotherapy. II. Plasma free hemoglobin and haptoglobin level changes.", "content": "The hemoglobin level of blood plasma is a sensitive tool in measuring radiation effects. Its value has increased in most of the cases during radiotherapy of cancer patients even applied at low doses. Haptoglobins behave similarly, the time-dependent changes during radiotherapy are almost identical at both parameters.", "contents": "Investigation of a valuable biochemical indicator in radiotherapy. II. Plasma free hemoglobin and haptoglobin level changes. The hemoglobin level of blood plasma is a sensitive tool in measuring radiation effects. Its value has increased in most of the cases during radiotherapy of cancer patients even applied at low doses. Haptoglobins behave similarly, the time-dependent changes during radiotherapy are almost identical at both parameters."}
{"id": "PMID:473262", "title": "Do intentions predict fertility? The experience in Taiwan, 1967-74.", "content": "Are responses to survey questions about desire for additional children useful in predicting future fertility? Data from Taiwan covering the years 1967--74, a period of rapid fertility decline and increasing contraceptive practice, indicate that reproductive intentions and contraceptive use were very good predictors of subsequent fertility. Whether more children were wanted was itself the most important determinant of contraceptive use. Aggregate consistency between attitudes and behavior was higher than found in US surveys during the same period. Similar studies are needed to determine whether statements of intentions have predictive value in other settings as well.", "contents": "Do intentions predict fertility? The experience in Taiwan, 1967-74. Are responses to survey questions about desire for additional children useful in predicting future fertility? Data from Taiwan covering the years 1967--74, a period of rapid fertility decline and increasing contraceptive practice, indicate that reproductive intentions and contraceptive use were very good predictors of subsequent fertility. Whether more children were wanted was itself the most important determinant of contraceptive use. Aggregate consistency between attitudes and behavior was higher than found in US surveys during the same period. Similar studies are needed to determine whether statements of intentions have predictive value in other settings as well."}
{"id": "PMID:473263", "title": "Abortion decision making: some findings from Colombia.", "content": "The reasons why some women seek to terminate an unwanted pregnancy, while others do not, were investigated in interviews with 108 women in Cali, Colombia (where abortion is illegal but reportedly widespread). All the women in the study had experienced an unwanted pregnancy, and in more than half the cases one major or several minor attempts were made to end it. The prospects for economic support from the father of the child emerged as the most important factor in decision making. Single women, separated women, and women in unions that seemed in danger of dissolving were most likely to take strong steps to terminate the unwanted pregnancy.", "contents": "Abortion decision making: some findings from Colombia. The reasons why some women seek to terminate an unwanted pregnancy, while others do not, were investigated in interviews with 108 women in Cali, Colombia (where abortion is illegal but reportedly widespread). All the women in the study had experienced an unwanted pregnancy, and in more than half the cases one major or several minor attempts were made to end it. The prospects for economic support from the father of the child emerged as the most important factor in decision making. Single women, separated women, and women in unions that seemed in danger of dissolving were most likely to take strong steps to terminate the unwanted pregnancy."}
{"id": "PMID:473285", "title": "Black suicide in the seventies: current trends.", "content": "In the most recent decade, the suicide rate among young blacks has risen to the point where it is nearly as high as that of their white peers. The data in this analysis reflect the striking contrast in age distribution in the suicide patterns of whites and blacks. Whereas white suicide increases in direct relation with advancing chronological age, suicide among blacks reaches its peak in the youthful years. Current statistics fail to reflect a \"dramatic\" or significant increase in the suicide rate of black women. Contrary to popular belief, black men in their twenties represent the most suicide-prone group. Young black males have been committing suicide at a steadily increasing rate during the past six years. Regionally, black suicide rates are highest in the North and West and lowest in the South. White suicide rates reflect a slightly different regional distribution. The highest rates occur in the West and the lowest in the North.", "contents": "Black suicide in the seventies: current trends. In the most recent decade, the suicide rate among young blacks has risen to the point where it is nearly as high as that of their white peers. The data in this analysis reflect the striking contrast in age distribution in the suicide patterns of whites and blacks. Whereas white suicide increases in direct relation with advancing chronological age, suicide among blacks reaches its peak in the youthful years. Current statistics fail to reflect a \"dramatic\" or significant increase in the suicide rate of black women. Contrary to popular belief, black men in their twenties represent the most suicide-prone group. Young black males have been committing suicide at a steadily increasing rate during the past six years. Regionally, black suicide rates are highest in the North and West and lowest in the South. White suicide rates reflect a slightly different regional distribution. The highest rates occur in the West and the lowest in the North."}
{"id": "PMID:473286", "title": "Some notes on conducting a psychological autopsy.", "content": "This paper outlines procedures for conducting interviews with relatives of suicide victims or survivors of equivocal deaths, by means of the \"psychological autopsy.\" A number of methodological recommendations are made which deal with training interviewers, contacting survivors and using experimental controls.", "contents": "Some notes on conducting a psychological autopsy. This paper outlines procedures for conducting interviews with relatives of suicide victims or survivors of equivocal deaths, by means of the \"psychological autopsy.\" A number of methodological recommendations are made which deal with training interviewers, contacting survivors and using experimental controls."}
{"id": "PMID:473287", "title": "Durkheim revisited: \"Why do women kill themselves?\".", "content": "Durkheim divided suicide into four social types; egoistic, anomic, fatalistic, and altruistic assigning the first two to modern, western society while relegating the last two to pre-industrial social orders. However, contemporary studies of female suicidal behavior and depression show that such women exhibit personality characteristics of low self-esteem, passivity, dependence and living vicariously for others which correspond to the behavioral indices of impersonalism, submissiveness, passivity, and obedience that produce the lack of individuation characteristic of Durkheim's altruistic/fatalistic suicide categories. On this basis, the author suggests that altruistic/fatalistic suicide may even in the modern world be relevant to the explanation of female suicidal behavior, a hypothesis which, if true, would support the contention that \"men and women inhibit different social worlds.\"", "contents": "Durkheim revisited: \"Why do women kill themselves?\". Durkheim divided suicide into four social types; egoistic, anomic, fatalistic, and altruistic assigning the first two to modern, western society while relegating the last two to pre-industrial social orders. However, contemporary studies of female suicidal behavior and depression show that such women exhibit personality characteristics of low self-esteem, passivity, dependence and living vicariously for others which correspond to the behavioral indices of impersonalism, submissiveness, passivity, and obedience that produce the lack of individuation characteristic of Durkheim's altruistic/fatalistic suicide categories. On this basis, the author suggests that altruistic/fatalistic suicide may even in the modern world be relevant to the explanation of female suicidal behavior, a hypothesis which, if true, would support the contention that \"men and women inhibit different social worlds.\""}
{"id": "PMID:473288", "title": "Suicide in France: some hypotheses.", "content": "The relatively high suicide rate in France is investigated and a number of influences are hypothesized as causative. These include, on a societal and demographic level, a history of high immigration, low emigration, a high proportion of old people, high urbanization, extraordinarily high alcoholism, and the extreme gap in income between upper and lower classes. The rigid bureaucracy of the state can leave the individual feeling infuriated and defeated. The legal system produces many injustices, such as long imprisonment without charges. The Church is liberal and supplies little prophylaxis against suicide. Overall, social integration must be judged to be low. Culturally, French values include an underlying pessimism, no strong fear of death, strong pressures to behave correctly and much malice toward neighbors. The modal personality structure contains defensive, constricted elements producing a vulnerable pseudo-autonomy. French child-rearing practices are effective in producing such personalities.", "contents": "Suicide in France: some hypotheses. The relatively high suicide rate in France is investigated and a number of influences are hypothesized as causative. These include, on a societal and demographic level, a history of high immigration, low emigration, a high proportion of old people, high urbanization, extraordinarily high alcoholism, and the extreme gap in income between upper and lower classes. The rigid bureaucracy of the state can leave the individual feeling infuriated and defeated. The legal system produces many injustices, such as long imprisonment without charges. The Church is liberal and supplies little prophylaxis against suicide. Overall, social integration must be judged to be low. Culturally, French values include an underlying pessimism, no strong fear of death, strong pressures to behave correctly and much malice toward neighbors. The modal personality structure contains defensive, constricted elements producing a vulnerable pseudo-autonomy. French child-rearing practices are effective in producing such personalities."}
{"id": "PMID:473289", "title": "Religiosity, fear of death and suicide acceptability.", "content": "The present research was an attempt to test two hypotheses derived from a recently proposed social psychological model of suicide: The acceptability of suicide is a decreasing function of religiosity and fear of death. Questionnaire data were collected for 205 undergraduates at a midwestern university in 1978. The questionnaire included several measures of religiosity, a factor analysis multidimensional fear of death scale, and a suicide acceptability scale. Results, showing that all of the religiosity measures and certain types of fear of death were significantly related to the acceptability of suicide, supported to the hypotheses under examination.", "contents": "Religiosity, fear of death and suicide acceptability. The present research was an attempt to test two hypotheses derived from a recently proposed social psychological model of suicide: The acceptability of suicide is a decreasing function of religiosity and fear of death. Questionnaire data were collected for 205 undergraduates at a midwestern university in 1978. The questionnaire included several measures of religiosity, a factor analysis multidimensional fear of death scale, and a suicide acceptability scale. Results, showing that all of the religiosity measures and certain types of fear of death were significantly related to the acceptability of suicide, supported to the hypotheses under examination."}
{"id": "PMID:473290", "title": "New approaches to crisis intervention.", "content": "Constant efforts to improve crisis services have led to many innovative programs. Some have proven their feasibility and become established procedures. Others are now in a developing stage and still others represent new approaches. A survey of 50 suicide prevention and crisis services around the world provides evidence of a trend toward a broadening range of services, a more active case-finding approach, greater visibility, increased integration into the community care system, and creative leadership by newer and smaller centers as well as the well-established ones. This is being accomplished without relinquishing the traditional respect for anonymity, ever-present availability, and a nonjudgmental regard for each person's need.", "contents": "New approaches to crisis intervention. Constant efforts to improve crisis services have led to many innovative programs. Some have proven their feasibility and become established procedures. Others are now in a developing stage and still others represent new approaches. A survey of 50 suicide prevention and crisis services around the world provides evidence of a trend toward a broadening range of services, a more active case-finding approach, greater visibility, increased integration into the community care system, and creative leadership by newer and smaller centers as well as the well-established ones. This is being accomplished without relinquishing the traditional respect for anonymity, ever-present availability, and a nonjudgmental regard for each person's need."}
{"id": "PMID:473305", "title": "The problem of human leptospirosis in Barbados.", "content": "The results of a retrospective study of human leptospirosis cases occurring in Barbados during the period 19-68-74, and of serological findings among high risk occupational groups, fever cases and health centre patients monitored for the disease during 1975-77, indicate that infection by serotype fort-bragg of the Autumnalis group is widespread in this Caribbean island. The occupational groups most affected are sanitation workers and sugar-cane workers. Results of serological tests conducted at the principle hospital are comparable with the results obtained by the microscopic agglutination reference test procedure conducted at the Pan American Zoonosis Centre (CEPANZO).", "contents": "The problem of human leptospirosis in Barbados. The results of a retrospective study of human leptospirosis cases occurring in Barbados during the period 19-68-74, and of serological findings among high risk occupational groups, fever cases and health centre patients monitored for the disease during 1975-77, indicate that infection by serotype fort-bragg of the Autumnalis group is widespread in this Caribbean island. The occupational groups most affected are sanitation workers and sugar-cane workers. Results of serological tests conducted at the principle hospital are comparable with the results obtained by the microscopic agglutination reference test procedure conducted at the Pan American Zoonosis Centre (CEPANZO)."}
{"id": "PMID:473306", "title": "Intestinal perforation in ascariasis--case reports.", "content": "Two cases of intestinal perforation in association with ascariasis are described. In both cases adult ascarids were found either in the gut or lying free in the peritoneal cavity. Neither patient had typhoid fever, one had Hodgkin's lymphoma. The authors believe that the perforation were directly caused by the ascarids. Both patients died.", "contents": "Intestinal perforation in ascariasis--case reports. Two cases of intestinal perforation in association with ascariasis are described. In both cases adult ascarids were found either in the gut or lying free in the peritoneal cavity. Neither patient had typhoid fever, one had Hodgkin's lymphoma. The authors believe that the perforation were directly caused by the ascarids. Both patients died."}
{"id": "PMID:473307", "title": "The surface antigen (HBsAg) and the e-antigen (HBeAg) in Somalian patients with acute viral hepatitis.", "content": "Serum samples from 55 Somalian patients with acute viral hepatitis were examined for the presence of the surface antigen (HBsAG) and the e-antigen (HBeAg) and the corresponding antibodies (anti-HBs and anti-e). No e-antigen was detected in patients with viral hepatitis or in controls (47 cases); anti-e was found in 23.6% of hepatitis cases, all of whom were HBsAg carriers, and in 10% of the controls. The HBsAg was found in 60% of cases with hepatitis and 34% of controls; the anti-HBs was detected in 18% of the patients with hepatitis, all except one of whom were negative for HBsAg; in the one exception the HBsAg and anti-HBs were present simultaneously; the anti-HBs was found in 44.6% of controls. The frequency of serological evidence of hepatitis B infection (HBV) based on the presence of antigen/antibody HBs was 78.2% among patients with acute viral hepatitis; only 30% of these patients showed evidence of anti-e antibody.", "contents": "The surface antigen (HBsAg) and the e-antigen (HBeAg) in Somalian patients with acute viral hepatitis. Serum samples from 55 Somalian patients with acute viral hepatitis were examined for the presence of the surface antigen (HBsAG) and the e-antigen (HBeAg) and the corresponding antibodies (anti-HBs and anti-e). No e-antigen was detected in patients with viral hepatitis or in controls (47 cases); anti-e was found in 23.6% of hepatitis cases, all of whom were HBsAg carriers, and in 10% of the controls. The HBsAg was found in 60% of cases with hepatitis and 34% of controls; the anti-HBs was detected in 18% of the patients with hepatitis, all except one of whom were negative for HBsAg; in the one exception the HBsAg and anti-HBs were present simultaneously; the anti-HBs was found in 44.6% of controls. The frequency of serological evidence of hepatitis B infection (HBV) based on the presence of antigen/antibody HBs was 78.2% among patients with acute viral hepatitis; only 30% of these patients showed evidence of anti-e antibody."}
{"id": "PMID:473308", "title": "The pathology and pathogenesis of fatal hepatic amoebiasis--A study based on 79 autopsy cases.", "content": "The present study is based on a retrospective analysis of 79 autopsy cases of hepatic amoebiasis. An attempt has been made to reconstruct the sequence of events starting from intestinal infection to invasion and transport of amoebae along the radicles of the portal veins, the formation of early Zahn's infarct and the proliferation of amoebae in such foci leading to the formation of small abscesses. The coalescence of small abscesses gives rise to the apparently large abscesses. Apart from direct contiguity, more distant extension leading to a satellite abscess is due to involvement of the hepatic and/or portal venous radicles. It seems that obstruction of the hepatic vein contributes substantially towards the enlargement of the liver and its exaggerated nutmeg appearance. Signs and symptoms of hepatic vein obstruction sometimes overshadow the abscess pathology. Thrombosis or pressure of a neighbouring abscess over the portal vein obstruction sometimes overshadow the abscess pathology. Thrombosis or pressure of a neighbouring abscess over the portal vein and bile-duct lead to development of portal hypertension and jaundice. Both cell-mediated and humoral immunity are depressed in fatal cases of hepatic amoebiasis.", "contents": "The pathology and pathogenesis of fatal hepatic amoebiasis--A study based on 79 autopsy cases. The present study is based on a retrospective analysis of 79 autopsy cases of hepatic amoebiasis. An attempt has been made to reconstruct the sequence of events starting from intestinal infection to invasion and transport of amoebae along the radicles of the portal veins, the formation of early Zahn's infarct and the proliferation of amoebae in such foci leading to the formation of small abscesses. The coalescence of small abscesses gives rise to the apparently large abscesses. Apart from direct contiguity, more distant extension leading to a satellite abscess is due to involvement of the hepatic and/or portal venous radicles. It seems that obstruction of the hepatic vein contributes substantially towards the enlargement of the liver and its exaggerated nutmeg appearance. Signs and symptoms of hepatic vein obstruction sometimes overshadow the abscess pathology. Thrombosis or pressure of a neighbouring abscess over the portal vein obstruction sometimes overshadow the abscess pathology. Thrombosis or pressure of a neighbouring abscess over the portal vein and bile-duct lead to development of portal hypertension and jaundice. Both cell-mediated and humoral immunity are depressed in fatal cases of hepatic amoebiasis."}
{"id": "PMID:473309", "title": "Malaria and haemoglobin A2 levels in northern Liberia.", "content": "The influence of malaria on HbA2 levels was investigated in two groups of children aged two to nine years from the Mano tribe of northern Liberia. One group, 174 children living in a town where there is malaria control, had a parasite rate of 6.5%, only a few having palpable spleens, but 282 children living in an area of intense malaria transmission had a parasite rate of 92%. All but one child in this group had enlarged spleens. However, the difference in proportions of elevated HbA2 values within the limits for beta-thalassaemia, 8% and 10.3% respectively, was not statistically significant (0.5 greater than P greater than 0.1). It was concluded that the influence of malaria on HbA2 levels is not significant and that this parameter is valid for detecting beta-thalassaemia trait in this population. Further, iron deficiency may be a more important factor than malaria to consider when assessing the results of HbA2 estimations.", "contents": "Malaria and haemoglobin A2 levels in northern Liberia. The influence of malaria on HbA2 levels was investigated in two groups of children aged two to nine years from the Mano tribe of northern Liberia. One group, 174 children living in a town where there is malaria control, had a parasite rate of 6.5%, only a few having palpable spleens, but 282 children living in an area of intense malaria transmission had a parasite rate of 92%. All but one child in this group had enlarged spleens. However, the difference in proportions of elevated HbA2 values within the limits for beta-thalassaemia, 8% and 10.3% respectively, was not statistically significant (0.5 greater than P greater than 0.1). It was concluded that the influence of malaria on HbA2 levels is not significant and that this parameter is valid for detecting beta-thalassaemia trait in this population. Further, iron deficiency may be a more important factor than malaria to consider when assessing the results of HbA2 estimations."}
{"id": "PMID:473310", "title": "Electrophoretic isoenzyme patterns of the pathogenic and non-pathogenic intestinal amoebae of man.", "content": "Cultured stocks of Entamoeba hartmanni, Endolimax nana, Iodamoeba buetschlli and Dientamoeba fragilis were compared with the four Entamoeba histolytical groups already described (SARGEAUNT et al., 1978), by the electrophoretic patterns of three enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM) and L-malate: NADP+ oxidoreductase (oxalacetate-decarboxylating) (ME). All the species were easily distinguished by their characteristic patterns.", "contents": "Electrophoretic isoenzyme patterns of the pathogenic and non-pathogenic intestinal amoebae of man. Cultured stocks of Entamoeba hartmanni, Endolimax nana, Iodamoeba buetschlli and Dientamoeba fragilis were compared with the four Entamoeba histolytical groups already described (SARGEAUNT et al., 1978), by the electrophoretic patterns of three enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM) and L-malate: NADP+ oxidoreductase (oxalacetate-decarboxylating) (ME). All the species were easily distinguished by their characteristic patterns."}
{"id": "PMID:473320", "title": "Epidemiological characteristics and clinical findings in patients with serologically proven toxocariasis.", "content": "The enzyme-linked immunosorbent assay (ELISA) using Toxocara canis larva-specific antigens was used to confirm a presumptive clinical diagnosis of visceral larva migrans in patients whose serum specimens were submitted to the Center for Disease Control. The clinical and epidemiological characteristics of patients with diagnostic ELISA titres (greater than or equal to log2 5) were compared with patients who had less or no detectable antibody. The typical patient with serologically confirmed toxocaral visceral larva migrans was about five years of age and resided in the southern half of the USA. Clinical findings were likely to include leucocytosis, eosinophilia, an increased anti-A or anti-B isohaemagglutinin titre, and an elevated serum IgG level. Pica, as ascertained by a physician questionnaire, was not a consistent finding and both sexes were nearly equally represented.", "contents": "Epidemiological characteristics and clinical findings in patients with serologically proven toxocariasis. The enzyme-linked immunosorbent assay (ELISA) using Toxocara canis larva-specific antigens was used to confirm a presumptive clinical diagnosis of visceral larva migrans in patients whose serum specimens were submitted to the Center for Disease Control. The clinical and epidemiological characteristics of patients with diagnostic ELISA titres (greater than or equal to log2 5) were compared with patients who had less or no detectable antibody. The typical patient with serologically confirmed toxocaral visceral larva migrans was about five years of age and resided in the southern half of the USA. Clinical findings were likely to include leucocytosis, eosinophilia, an increased anti-A or anti-B isohaemagglutinin titre, and an elevated serum IgG level. Pica, as ascertained by a physician questionnaire, was not a consistent finding and both sexes were nearly equally represented."}
{"id": "PMID:473321", "title": "Isoelectric focusing of some enzymes from Echinococcus granulosus (horse and sheep strains) and E. multilocularis.", "content": "Extracts of the horse and sheep strains of Echinococcus granulosus and E. multilocularis were compared on the basis of their isoenzyme patterns for 10 enzymes by means of isoelectric focusing in polyacrylamide gels. The enzymes examined were: acid phosphatase, lactate dehydrogenase, malate dehydrogenase, malic enzyme, phosphoglucoseisomerase, isocitrate dehydrogenase, adenylate kinase, aldolase and alpha-glycerophosphate dehydrogenase. Interspecific and intraspecific differences are apparent in the isoenzyme profiles of all the enzymes except adenylate kinase; the pattern and activity of adenylate kinase are identical for both strains of E. granulosus but this enzyme clearly distinguishes these forms from E. multilocularis. The absence of electromorphic variation in any of the enzymes from either form of E. granulosus may be a result of the self-fertilizing hermaphraditism of these organisms.", "contents": "Isoelectric focusing of some enzymes from Echinococcus granulosus (horse and sheep strains) and E. multilocularis. Extracts of the horse and sheep strains of Echinococcus granulosus and E. multilocularis were compared on the basis of their isoenzyme patterns for 10 enzymes by means of isoelectric focusing in polyacrylamide gels. The enzymes examined were: acid phosphatase, lactate dehydrogenase, malate dehydrogenase, malic enzyme, phosphoglucoseisomerase, isocitrate dehydrogenase, adenylate kinase, aldolase and alpha-glycerophosphate dehydrogenase. Interspecific and intraspecific differences are apparent in the isoenzyme profiles of all the enzymes except adenylate kinase; the pattern and activity of adenylate kinase are identical for both strains of E. granulosus but this enzyme clearly distinguishes these forms from E. multilocularis. The absence of electromorphic variation in any of the enzymes from either form of E. granulosus may be a result of the self-fertilizing hermaphraditism of these organisms."}
{"id": "PMID:473322", "title": "Effect of corticosteroid and irradiation on caecal amoebic infection in rats.", "content": "Albino rats (Wistar strain) were pretreated with corticosteroid, irradiation or both to study the effect on the outcome of amoebic infection given intracaecally. The number of animals with lesions amongst \"treated\" groups was not significantly different from that amongst untreated control animals (P greater than 0.05) but amoebic pathology was markedly exacerbated amongst the treated animals. It is thus thought that once the amoebic infection is established, amoebae become better established for penetration in the immuno-depressed host. The study further suggests that corticosteroid therapy could aggravate an otherwise sub-clinical amoebic infection in man.", "contents": "Effect of corticosteroid and irradiation on caecal amoebic infection in rats. Albino rats (Wistar strain) were pretreated with corticosteroid, irradiation or both to study the effect on the outcome of amoebic infection given intracaecally. The number of animals with lesions amongst \"treated\" groups was not significantly different from that amongst untreated control animals (P greater than 0.05) but amoebic pathology was markedly exacerbated amongst the treated animals. It is thus thought that once the amoebic infection is established, amoebae become better established for penetration in the immuno-depressed host. The study further suggests that corticosteroid therapy could aggravate an otherwise sub-clinical amoebic infection in man."}
{"id": "PMID:473323", "title": "Skin sensitivity tests in Nigerians with bronchial asthma.", "content": "Skin sensitivity tests using the prick method were performed on 174 patients with bronchial asthma and 160 controls, using 20 common allergens. The results revealed a high incidence of positive skin test reactions to Dermatophagoides pteronyssinus, grass and flower pollens, feathers, and some ingesta such as cow's milk and fish. The difference in the reactions between the asthmatic patients and the controls was statistically significant. The results suggest that house-dust mite allergy is the most significant factor in the aetiology of bronchial asthma in Nigerians.", "contents": "Skin sensitivity tests in Nigerians with bronchial asthma. Skin sensitivity tests using the prick method were performed on 174 patients with bronchial asthma and 160 controls, using 20 common allergens. The results revealed a high incidence of positive skin test reactions to Dermatophagoides pteronyssinus, grass and flower pollens, feathers, and some ingesta such as cow's milk and fish. The difference in the reactions between the asthmatic patients and the controls was statistically significant. The results suggest that house-dust mite allergy is the most significant factor in the aetiology of bronchial asthma in Nigerians."}
{"id": "PMID:473324", "title": "Maternal deaths in the Kilimanjaro region of Tanzania.", "content": "The maternal deaths in a supra-regional reference hospital between 1971 and 1977 are reviewed and avoidable factors discussed. Improvements in ante-natal care have diminished the number of deaths from obstetric causes and as a result deaths from associated diseases are assuming greater significance. Particular attention is drawn to the enterocolitis group of diseases which account for 15% of the total deaths in this series. The effect of pregnancy on amoebic colitis has not been widely appreciated.", "contents": "Maternal deaths in the Kilimanjaro region of Tanzania. The maternal deaths in a supra-regional reference hospital between 1971 and 1977 are reviewed and avoidable factors discussed. Improvements in ante-natal care have diminished the number of deaths from obstetric causes and as a result deaths from associated diseases are assuming greater significance. Particular attention is drawn to the enterocolitis group of diseases which account for 15% of the total deaths in this series. The effect of pregnancy on amoebic colitis has not been widely appreciated."}
{"id": "PMID:473325", "title": "A new species of Leishmania parasite from the Venezuelan Andes region.", "content": "A new form of Leishmania is described from the Venezuelan Andes region. L. garnhami n.sp. is proposed for this parasite with amastigote stages showing a peculiar and unique organelle seen with light and electron microscope. It produces cutaneous lesions in people living at a height of between 800 and 1,8000 m. in urban and rural areas; the disease is associated with Lutzomyia townsendi, the main anthropophilic sandfly in the region. The parasite is easily inoculable into hamsters and grows slowly in vitro in blood-agar media with glucose, but more prolifically without glucose.", "contents": "A new species of Leishmania parasite from the Venezuelan Andes region. A new form of Leishmania is described from the Venezuelan Andes region. L. garnhami n.sp. is proposed for this parasite with amastigote stages showing a peculiar and unique organelle seen with light and electron microscope. It produces cutaneous lesions in people living at a height of between 800 and 1,8000 m. in urban and rural areas; the disease is associated with Lutzomyia townsendi, the main anthropophilic sandfly in the region. The parasite is easily inoculable into hamsters and grows slowly in vitro in blood-agar media with glucose, but more prolifically without glucose."}
{"id": "PMID:473326", "title": "Leptospirosis in patients from Grenada, West Indies.", "content": "Evidence of leptospirosis in humans from Grenada, W.I., is reported here for the first time. Of 139 hospital patients with pyrexia of undetermined origin, 94 were jaundiced, and 16 (17%) of these were positive for hepatitis B antigen (HBsAg) and not examined further. 20 of the remaining 123 (16%) febrile patients were positive for leptospiral antibodies; 11 of them were current or recent cases. 25 of 182 (14%) afebrile out-patients showed evidence of previous exposure to leptospiral organisms. The most frequently encountered serogroups in the 45 seropositive in- and out-patients were Icterohaemorrhagiae (38%), Panama (24%) and Canicola (11%).", "contents": "Leptospirosis in patients from Grenada, West Indies. Evidence of leptospirosis in humans from Grenada, W.I., is reported here for the first time. Of 139 hospital patients with pyrexia of undetermined origin, 94 were jaundiced, and 16 (17%) of these were positive for hepatitis B antigen (HBsAg) and not examined further. 20 of the remaining 123 (16%) febrile patients were positive for leptospiral antibodies; 11 of them were current or recent cases. 25 of 182 (14%) afebrile out-patients showed evidence of previous exposure to leptospiral organisms. The most frequently encountered serogroups in the 45 seropositive in- and out-patients were Icterohaemorrhagiae (38%), Panama (24%) and Canicola (11%)."}
{"id": "PMID:473327", "title": "Faecal pollution of surface waters in Jakarta.", "content": "Profuse numbers of Enterobacteriaceae were found in samples of surface waters from the Ciliwung River and adjoining canals which criss-cross metropolitan Jakarta and are used widely for domestic purposes by the poorer sections of its population. 15 of the 20 specimens gres E. coli, Klebsiella were isolated from seven and Citrobacter from four. Using enrichment culture procedures, Salmonella sp. were grown from 10 (48%) of 21 water samples examined, and 12 (63%) of aquatic sediments collected at the same sites. Altogether 14 serotypes and 37 Salmonella isolations were recorded. This high degree of faecal contamination of the environment is a major cause of the immense problem of gastro-intestinal infections in that city and probably in many similar cities in developing countries in the tropics.", "contents": "Faecal pollution of surface waters in Jakarta. Profuse numbers of Enterobacteriaceae were found in samples of surface waters from the Ciliwung River and adjoining canals which criss-cross metropolitan Jakarta and are used widely for domestic purposes by the poorer sections of its population. 15 of the 20 specimens gres E. coli, Klebsiella were isolated from seven and Citrobacter from four. Using enrichment culture procedures, Salmonella sp. were grown from 10 (48%) of 21 water samples examined, and 12 (63%) of aquatic sediments collected at the same sites. Altogether 14 serotypes and 37 Salmonella isolations were recorded. This high degree of faecal contamination of the environment is a major cause of the immense problem of gastro-intestinal infections in that city and probably in many similar cities in developing countries in the tropics."}
{"id": "PMID:473328", "title": "Incidence of abnormal haemoglobin traits among Gambian children.", "content": "The electrophoretic mobility of haemoglobin from 343 Gambian children between the ages of two months and ten years has been investigated. The incidence of Hb-S and Hb-C traits is described and compared with earlier reports on the distribution of these traits in The Gambia. One child was identified as having the rare haemoglobin, Hb-A + N.", "contents": "Incidence of abnormal haemoglobin traits among Gambian children. The electrophoretic mobility of haemoglobin from 343 Gambian children between the ages of two months and ten years has been investigated. The incidence of Hb-S and Hb-C traits is described and compared with earlier reports on the distribution of these traits in The Gambia. One child was identified as having the rare haemoglobin, Hb-A + N."}
{"id": "PMID:473329", "title": "Trypanosoma brucei: Miniature anion-exchange centrifugation technique for detection of low parasitaemias: Adaptation for field use.", "content": "The miniature anion-exchange/centrifugation (AEC) method, originally developed for the detection of submicroscopic trypanosomaemias in laboratory rodents, has been adapted for the diagnosis of trypanosomiasis in man in the field using blood samples obtained by finger-prick. It has been tested in a survey in The Gambia. The method is shown to be highly sensitive and to fulfil the first essential criteria for exploitation in the field, namely, that it can be operated in the open air under tropical conditions, and that an adequate number of subjects can be examined in a normal working day at an acceptable cost. The method also offers two advantages over the other highly sensitive method applicable to small blood samples, the microhaematocrit buffy-coat microscopy (MBCM) method, namely, that it minimized the requirements for highly critical microscopy and provides, in the same operation, samples of diluted plasma which can be used for serological study.", "contents": "Trypanosoma brucei: Miniature anion-exchange centrifugation technique for detection of low parasitaemias: Adaptation for field use. The miniature anion-exchange/centrifugation (AEC) method, originally developed for the detection of submicroscopic trypanosomaemias in laboratory rodents, has been adapted for the diagnosis of trypanosomiasis in man in the field using blood samples obtained by finger-prick. It has been tested in a survey in The Gambia. The method is shown to be highly sensitive and to fulfil the first essential criteria for exploitation in the field, namely, that it can be operated in the open air under tropical conditions, and that an adequate number of subjects can be examined in a normal working day at an acceptable cost. The method also offers two advantages over the other highly sensitive method applicable to small blood samples, the microhaematocrit buffy-coat microscopy (MBCM) method, namely, that it minimized the requirements for highly critical microscopy and provides, in the same operation, samples of diluted plasma which can be used for serological study."}
{"id": "PMID:473341", "title": "Discocyte--echinocyte reversibility in blood stored in CPD over a period of 56 days.", "content": "Blood collected with CPD and stored was examined with optical (LM) and scanning electron microscopy (SEM) before and after reversal of echinocytes into discocytes. Reversal was achieved by incubation of the red blood cells at 37 C in an adenosine containing medium. The transformation of discocytes into echinocytes occurred rapidly during the first three weeks of storage. A shape/density relationship was observed in the various fractions. The denser cells were found to have the more advanced echinocytic changes. After incubation with adenosine, most of the cells reversed into discocytes and early stages of stomatocytes. When spheroechinocytes I and II were present, they reversed into spherostomatocytes. No echinocytogenic property was found in plasma during 5 weeks of storage at 4 C and no immediate reversion of echinocytes was obtained in fresh plasma, therefore the initial discocyte--echinocyte transformation was explained by intracellular changes. The data from the various fractions showed that the more dense cells were more spherocytic. We suggest that removal of this part of the population of stored red blood cells might improve the survival of transfused cells.", "contents": "Discocyte--echinocyte reversibility in blood stored in CPD over a period of 56 days. Blood collected with CPD and stored was examined with optical (LM) and scanning electron microscopy (SEM) before and after reversal of echinocytes into discocytes. Reversal was achieved by incubation of the red blood cells at 37 C in an adenosine containing medium. The transformation of discocytes into echinocytes occurred rapidly during the first three weeks of storage. A shape/density relationship was observed in the various fractions. The denser cells were found to have the more advanced echinocytic changes. After incubation with adenosine, most of the cells reversed into discocytes and early stages of stomatocytes. When spheroechinocytes I and II were present, they reversed into spherostomatocytes. No echinocytogenic property was found in plasma during 5 weeks of storage at 4 C and no immediate reversion of echinocytes was obtained in fresh plasma, therefore the initial discocyte--echinocyte transformation was explained by intracellular changes. The data from the various fractions showed that the more dense cells were more spherocytic. We suggest that removal of this part of the population of stored red blood cells might improve the survival of transfused cells."}
{"id": "PMID:473342", "title": "Factors that influence the process of 51chromium labeling of human granulocytes isolated from blood by counterflow centrifugation.", "content": "The uptake of 51chromium by human granulocytes isolated from blood by counterflow centrifugation is influenced by the composition of the suspension medium, the incubation period, the temperature and the amount of 51chromium used. Counterflow centrifugation-elutriation isolated approximately 98 per cent of the granulocytes from the blood. Acid-citrate-dextrose (ACD) plasma, citrate-phosphate-dextrose (CPD) plasma and heparinized plasma as resuspension media produced similar labeling results, whereas phosphate-buffered saline resulted in three times greater uptake in 51chromium. The greatest uptake of 51chromium (4%) was achieved after incubation of 37 C for one hour in autologous plasma, and granulocyte integrity and metabolic function in vitro were not adversely affected.", "contents": "Factors that influence the process of 51chromium labeling of human granulocytes isolated from blood by counterflow centrifugation. The uptake of 51chromium by human granulocytes isolated from blood by counterflow centrifugation is influenced by the composition of the suspension medium, the incubation period, the temperature and the amount of 51chromium used. Counterflow centrifugation-elutriation isolated approximately 98 per cent of the granulocytes from the blood. Acid-citrate-dextrose (ACD) plasma, citrate-phosphate-dextrose (CPD) plasma and heparinized plasma as resuspension media produced similar labeling results, whereas phosphate-buffered saline resulted in three times greater uptake in 51chromium. The greatest uptake of 51chromium (4%) was achieved after incubation of 37 C for one hour in autologous plasma, and granulocyte integrity and metabolic function in vitro were not adversely affected."}
{"id": "PMID:473343", "title": "The role of disseminated intravascular coagulation in shock induced by transfusion of human blood in dogs.", "content": "Hemolytic blood transfusion reactions were produced in 20 dogs. Animals transfused with human blood twice in a two-week interval demonstrated a significant increase in anti-human red blood cell agglutination titer at the time of the second transfusion. Eighteen dogs that survived from the first transfusion reaction were divided into two groups with or without heparin treatment. The hemolytic transfusion reaction in the sensitized animals was characterized by shock and multiple coagulation abnormalities. Heparin failed to prevent shock, hemoglobinemia and thrombocytopenia, and decreased the mortality rate in heparinized animals. Pathologic examination revealed that the thrombi in the splanchnic organs were less common in the heparinized dogs.", "contents": "The role of disseminated intravascular coagulation in shock induced by transfusion of human blood in dogs. Hemolytic blood transfusion reactions were produced in 20 dogs. Animals transfused with human blood twice in a two-week interval demonstrated a significant increase in anti-human red blood cell agglutination titer at the time of the second transfusion. Eighteen dogs that survived from the first transfusion reaction were divided into two groups with or without heparin treatment. The hemolytic transfusion reaction in the sensitized animals was characterized by shock and multiple coagulation abnormalities. Heparin failed to prevent shock, hemoglobinemia and thrombocytopenia, and decreased the mortality rate in heparinized animals. Pathologic examination revealed that the thrombi in the splanchnic organs were less common in the heparinized dogs."}
{"id": "PMID:473346", "title": "Target inventory levels for a hospital blood bank or a decentralized regional blood banking system.", "content": "For any blood type, there is a complex interaction among the optimal inventory level, daily demand level, the transfusion to crossmatch ratio, the crossmatch release period and the age of arriving units that determine the shortage and outdate rate. The blood bank administrator should establish optimal target inventory levels based on a simple equation (decision rule) relating these factors. Evaluation of this rule indicates that its implementation can lead to a very low shortage rate and a reasonable low outdate rate if the blood bank administrator makes efforts to control the crossmatch release period and the average transfusion to crossmatch ratio.", "contents": "Target inventory levels for a hospital blood bank or a decentralized regional blood banking system. For any blood type, there is a complex interaction among the optimal inventory level, daily demand level, the transfusion to crossmatch ratio, the crossmatch release period and the age of arriving units that determine the shortage and outdate rate. The blood bank administrator should establish optimal target inventory levels based on a simple equation (decision rule) relating these factors. Evaluation of this rule indicates that its implementation can lead to a very low shortage rate and a reasonable low outdate rate if the blood bank administrator makes efforts to control the crossmatch release period and the average transfusion to crossmatch ratio."}
{"id": "PMID:473340", "title": "Therapeutic transfusions of previously frozen washed human platelets.", "content": "The platelets used in this study were collected by serial centrifugation, and within four hours of collection were frozen with 5% dimethylsulfoxide (DMSO) at an overall rate of 2 to 3 C per minute by storage in a mechanical refrigerator at -80 C. The frozen platelets were stored for four to ten weeks before thawing and washing. After washing, the units were kept at room temperature for six to eight hours before transfusion. The units were pooled, and an average of eight units was given to each of four patients, with a range of three to 14 units per transfusion. In vitro recovery after washing was about 65 per cent and in vivo recovery of the 51chromium labeled (51Cr) platelets was about 35 per cent. The infusion of these previously frozen washed platelets corrected prolonged bleeding times in patients. The homologous platelets were transfused along with other blood products to treat patients with hematologic disorders. The circulation and function of the donor platelets were influenced by compatibility of the platelets, the quality of platelet preservation and the patient's disease state.", "contents": "Therapeutic transfusions of previously frozen washed human platelets. The platelets used in this study were collected by serial centrifugation, and within four hours of collection were frozen with 5% dimethylsulfoxide (DMSO) at an overall rate of 2 to 3 C per minute by storage in a mechanical refrigerator at -80 C. The frozen platelets were stored for four to ten weeks before thawing and washing. After washing, the units were kept at room temperature for six to eight hours before transfusion. The units were pooled, and an average of eight units was given to each of four patients, with a range of three to 14 units per transfusion. In vitro recovery after washing was about 65 per cent and in vivo recovery of the 51chromium labeled (51Cr) platelets was about 35 per cent. The infusion of these previously frozen washed platelets corrected prolonged bleeding times in patients. The homologous platelets were transfused along with other blood products to treat patients with hematologic disorders. The circulation and function of the donor platelets were influenced by compatibility of the platelets, the quality of platelet preservation and the patient's disease state."}
{"id": "PMID:473347", "title": "A regional blood management system with prescheduled deliveries.", "content": "The prescheduling of blood deliveries from regional blood centers can improve blood availability at all transfusion services while reducing outdating, delivery costs and inventories maintained at region blood centers. A procedure for determining optimal distribution policies that maintain the highest possible blood availability at transfusion services while holding the achievable outdating level to a small percentage of the total usage is described. The manner in which this plan is being implemented in Long Island, New York, is presented, as well as a discussion of some preliminary results.", "contents": "A regional blood management system with prescheduled deliveries. The prescheduling of blood deliveries from regional blood centers can improve blood availability at all transfusion services while reducing outdating, delivery costs and inventories maintained at region blood centers. A procedure for determining optimal distribution policies that maintain the highest possible blood availability at transfusion services while holding the achievable outdating level to a small percentage of the total usage is described. The manner in which this plan is being implemented in Long Island, New York, is presented, as well as a discussion of some preliminary results."}
{"id": "PMID:473348", "title": "Blood donorship and psychological reactance.", "content": "Research in social psychology suggests that expressing urgent need in requests for blood donation may result in less volunteering to help, not more. In addition, there is evidence that requests for several donations over a period of time are more likely to meet with refusal than requests for only one donation. These issues were investigated within the framework of a social psychological theory known as reactance theory.", "contents": "Blood donorship and psychological reactance. Research in social psychology suggests that expressing urgent need in requests for blood donation may result in less volunteering to help, not more. In addition, there is evidence that requests for several donations over a period of time are more likely to meet with refusal than requests for only one donation. These issues were investigated within the framework of a social psychological theory known as reactance theory."}
{"id": "PMID:473349", "title": "Platelet glycolysis in platelet storage. II. Levels and turnover of metabolic intermediates.", "content": "The metabolism of freshly collected platelets and platelets stored for three or four days at room temperature has been investigated. The levels of glycolytic intermediates were measured in platelets before and after incubation for 15 minutes in a buffered aerobic medium. Platelet intermediates were relatively well maintained, and levels of intermediates that were partially depleted, such as ATP, were restored toward normal after short term incubation even after storage for four days. Incorporation of 32PO4 into fresh and stored platelets during incubation in aerobic buffered medium was also studied. The incorporation pattern was only very modestly influenced by storage. These studies suggest that changes in glycolytic capacity do not play a major role in the loss of viability of platelets during storage at room temperature.", "contents": "Platelet glycolysis in platelet storage. II. Levels and turnover of metabolic intermediates. The metabolism of freshly collected platelets and platelets stored for three or four days at room temperature has been investigated. The levels of glycolytic intermediates were measured in platelets before and after incubation for 15 minutes in a buffered aerobic medium. Platelet intermediates were relatively well maintained, and levels of intermediates that were partially depleted, such as ATP, were restored toward normal after short term incubation even after storage for four days. Incorporation of 32PO4 into fresh and stored platelets during incubation in aerobic buffered medium was also studied. The incorporation pattern was only very modestly influenced by storage. These studies suggest that changes in glycolytic capacity do not play a major role in the loss of viability of platelets during storage at room temperature."}
{"id": "PMID:473350", "title": "Filtration leukapheresis: the influence of granulocyte counts upon the donors' yield.", "content": "The purpose of this investigation was to determine the influence of granulocyte count on the yield of granulocytes. Healthy adult volunteer donors underwent filtration leukapheresis without premedication. The granulocyte count was measured before and immediately after the procedure. In 73 donors, there was an increase and in 67 donors there was a decrease in the granulocyte counts. In this study of nonmedicated donors those with high initial granulocytes counts and those in which there was a large increase in the postleukapheresis granulocyte counts provided the best yield with the filtration leukapheresis technique.", "contents": "Filtration leukapheresis: the influence of granulocyte counts upon the donors' yield. The purpose of this investigation was to determine the influence of granulocyte count on the yield of granulocytes. Healthy adult volunteer donors underwent filtration leukapheresis without premedication. The granulocyte count was measured before and immediately after the procedure. In 73 donors, there was an increase and in 67 donors there was a decrease in the granulocyte counts. In this study of nonmedicated donors those with high initial granulocytes counts and those in which there was a large increase in the postleukapheresis granulocyte counts provided the best yield with the filtration leukapheresis technique."}
{"id": "PMID:473351", "title": "Three additional examples of anti-Xga and Xg blood groups among the Japanese.", "content": "Three examples of anti-Xga identified in Japanese donors are reported. Two of these were stimulated by pregnancy, while the antibody stimulation of the other one remains unknown. All of these antibodies had the ability to bind complement. A dosage effect was observed in an anti-Xga serum. The Xga gene frequency for 529 unrelated Japanese was 0.681, similar to that for Caucasians. Tests on 125 Japanese families with 242 offspring for Xga antigen are also reported.", "contents": "Three additional examples of anti-Xga and Xg blood groups among the Japanese. Three examples of anti-Xga identified in Japanese donors are reported. Two of these were stimulated by pregnancy, while the antibody stimulation of the other one remains unknown. All of these antibodies had the ability to bind complement. A dosage effect was observed in an anti-Xga serum. The Xga gene frequency for 529 unrelated Japanese was 0.681, similar to that for Caucasians. Tests on 125 Japanese families with 242 offspring for Xga antigen are also reported."}
{"id": "PMID:473352", "title": "Priapism during filtration leukapheresis.", "content": "Priapism has been observed during two out of 3,680 filtration leukapheresis procedures in male donors and has been reported during hemodialysis. Both procedures are associated with enhanced granulocyte adhesion and aggregation presumably due to C5a. During both procedures, heparin is administered and this drug has been shown to cause heparin-dependent anti-platelet antibodies. It is suggested that complement mediated venous leukostasis or immune-induced platelet aggregates might impair the normal blood flow from the penis and result in a state of priapism.", "contents": "Priapism during filtration leukapheresis. Priapism has been observed during two out of 3,680 filtration leukapheresis procedures in male donors and has been reported during hemodialysis. Both procedures are associated with enhanced granulocyte adhesion and aggregation presumably due to C5a. During both procedures, heparin is administered and this drug has been shown to cause heparin-dependent anti-platelet antibodies. It is suggested that complement mediated venous leukostasis or immune-induced platelet aggregates might impair the normal blood flow from the penis and result in a state of priapism."}
{"id": "PMID:473378", "title": "Tuberculous osteitis of the talus.", "content": "A case of tuberculous osteitis of the talus in a 6-year-old white male is reported. Osteitis of the talus is uncommon and tuberculous disease of this bone is now rare. There was a delay of 5 weeks between presentation and final diagnosis and the reasons for this delay are discussed. Further investigation showed the presence of tuberculosis of the fifth lumbar vertebra and of the left kidney. With prompt chemotherapy and early surgery, the long term results of this once crippling disease are now excellent.", "contents": "Tuberculous osteitis of the talus. A case of tuberculous osteitis of the talus in a 6-year-old white male is reported. Osteitis of the talus is uncommon and tuberculous disease of this bone is now rare. There was a delay of 5 weeks between presentation and final diagnosis and the reasons for this delay are discussed. Further investigation showed the presence of tuberculosis of the fifth lumbar vertebra and of the left kidney. With prompt chemotherapy and early surgery, the long term results of this once crippling disease are now excellent."}
{"id": "PMID:473379", "title": "Infections with Mycobacterium malmoense in England and Wales.", "content": "Mycobacterium malmoense has been isolated from 11 patients in England and Wales. It was thought to be the cause of pulmonary disease in 9 of them and cervical adenitis in 2. The cultural and biochemical characters of the species are described and its position in relation to the avium-intracellulare complex and the non-chromogenicum-terrae-triviale complex determined by matching coefficients using 88 characters. Brief details are given of the 11 cases.", "contents": "Infections with Mycobacterium malmoense in England and Wales. Mycobacterium malmoense has been isolated from 11 patients in England and Wales. It was thought to be the cause of pulmonary disease in 9 of them and cervical adenitis in 2. The cultural and biochemical characters of the species are described and its position in relation to the avium-intracellulare complex and the non-chromogenicum-terrae-triviale complex determined by matching coefficients using 88 characters. Brief details are given of the 11 cases."}
{"id": "PMID:473380", "title": "Water: the natural habitat of Mycobacterium kansasii?", "content": "It has been demonstrated that, after inoculation, Mycobacterium kansasii will survive in water up to 12 months without any change in cultural or lipid characteristics. The organism failed to survive in soil. It is suggested that the natural habitat of M. kansasii is water.", "contents": "Water: the natural habitat of Mycobacterium kansasii? It has been demonstrated that, after inoculation, Mycobacterium kansasii will survive in water up to 12 months without any change in cultural or lipid characteristics. The organism failed to survive in soil. It is suggested that the natural habitat of M. kansasii is water."}
{"id": "PMID:473381", "title": "Lymph node tuberculosis: a comparison of treatments 18 months after completion of chemotherapy.", "content": "Ninety patients with lymph node tuberculosis were treated with either rifampicin and isoniazid or ethambutol and isoniazid for 18 months, supplemented by sheptomycin for the first two months. No differences emerged between the two regimens, either during treatment or during an 18 month post-chemotherapy follow-up. In 7% of patients lymph nodes enlarged transiently after the end of treatment and in 7% enlarged nodes persisted. No patient required further treatment. One patient was left with an intermittently discharging sinus in an operation scar.", "contents": "Lymph node tuberculosis: a comparison of treatments 18 months after completion of chemotherapy. Ninety patients with lymph node tuberculosis were treated with either rifampicin and isoniazid or ethambutol and isoniazid for 18 months, supplemented by sheptomycin for the first two months. No differences emerged between the two regimens, either during treatment or during an 18 month post-chemotherapy follow-up. In 7% of patients lymph nodes enlarged transiently after the end of treatment and in 7% enlarged nodes persisted. No patient required further treatment. One patient was left with an intermittently discharging sinus in an operation scar."}
{"id": "PMID:473382", "title": "A comparison of Mantoux and tuberculin Tine testing in a chest unit.", "content": "Simultaneous Mantoux (10 T.U.) and Tuberculin Tine tests were performed on 393 in-patients on a chest unit. The results were read by the same observer between 48 and 72 hours later. The overall agreement between the tests was 78%; this rose to 90% if patients categorised as 'doubtful' reactors were reclassified as positive. Where one test was 'doubtful' the other was more than 3 times as likely to be positive as negative. It is suggested that the 'doubtful' category by abandoned for both tests and readings in this range be reclassified as positive. In patients expected on clinical grounds to be positive reactors, over one-third were negative to both tests; in those expected to be negative over one-fifth were positive. The Tine test is convenient and gives results which seem to correlate well with the Mantoux test, although neither test appears to be highly specific.", "contents": "A comparison of Mantoux and tuberculin Tine testing in a chest unit. Simultaneous Mantoux (10 T.U.) and Tuberculin Tine tests were performed on 393 in-patients on a chest unit. The results were read by the same observer between 48 and 72 hours later. The overall agreement between the tests was 78%; this rose to 90% if patients categorised as 'doubtful' reactors were reclassified as positive. Where one test was 'doubtful' the other was more than 3 times as likely to be positive as negative. It is suggested that the 'doubtful' category by abandoned for both tests and readings in this range be reclassified as positive. In patients expected on clinical grounds to be positive reactors, over one-third were negative to both tests; in those expected to be negative over one-fifth were positive. The Tine test is convenient and gives results which seem to correlate well with the Mantoux test, although neither test appears to be highly specific."}
{"id": "PMID:473383", "title": "[Production and properties of trypsin immobilized on a polyurethane matrix].", "content": "Two methods were used to immobilize trypsin on the polyurethane carrier on the basis of toluylenediisocyanatepolyoxypropylene glycol due to interaction between the lyzine free amino groups and the enzyme arginine guanidine group and the prepolymer isocyanate group. The amount of the enzyme chemically bound by the two methods is about 50 and more than 70%, respectively. The substrate specificity of the initial and washed samples of the immobilized trypsin was studied with respect to three highly molecular substrates with different molecular weight and different charges--protamine, casein, hemoglobin. It is shown that independently of the method of binding the activity of the immobilized trypsin is the highest with respect to hemoglobin and is the lowest with respect to protamine. The samples of trypsin immobilized on the polyurethane carrier may be used in biology and medicine when creating the prolonged forms of the enzymic preparations.", "contents": "[Production and properties of trypsin immobilized on a polyurethane matrix]. Two methods were used to immobilize trypsin on the polyurethane carrier on the basis of toluylenediisocyanatepolyoxypropylene glycol due to interaction between the lyzine free amino groups and the enzyme arginine guanidine group and the prepolymer isocyanate group. The amount of the enzyme chemically bound by the two methods is about 50 and more than 70%, respectively. The substrate specificity of the initial and washed samples of the immobilized trypsin was studied with respect to three highly molecular substrates with different molecular weight and different charges--protamine, casein, hemoglobin. It is shown that independently of the method of binding the activity of the immobilized trypsin is the highest with respect to hemoglobin and is the lowest with respect to protamine. The samples of trypsin immobilized on the polyurethane carrier may be used in biology and medicine when creating the prolonged forms of the enzymic preparations."}
{"id": "PMID:473384", "title": "[Trypsin immobilization on organo-silica surface].", "content": "The methods are suggested for immobilizing trypsin on the organo-silica surface by means of cyanuric chloride and maleic anhydride. The analysis of kinetics of the enzyme carrier coupling shows that at 22 degrees C the immobilization time on the surface of organo-silicas activated with cyanuric chloride and maleic anhydride is 70 and 35 min, respectively. The immobilized trypsin is shown to be more thermostable as compared to the soluble one. The half-life of the immobilized trypsin at 55 degrees C is about 40 h.", "contents": "[Trypsin immobilization on organo-silica surface]. The methods are suggested for immobilizing trypsin on the organo-silica surface by means of cyanuric chloride and maleic anhydride. The analysis of kinetics of the enzyme carrier coupling shows that at 22 degrees C the immobilization time on the surface of organo-silicas activated with cyanuric chloride and maleic anhydride is 70 and 35 min, respectively. The immobilized trypsin is shown to be more thermostable as compared to the soluble one. The half-life of the immobilized trypsin at 55 degrees C is about 40 h."}
{"id": "PMID:473385", "title": "[Affinity sorbents for obtaining plasminogen].", "content": "Using immobilization of l-lysine on cellulose and CM-Cellulose activated with bromo-cyanogen and dicyclohexyl carboxyimide, respectively, the affined sorbents are obtained and possibility of their application for obtaining the high-purified plasminogen is studied. According to the yield and activity of plasminogen, l-lysine-cellulose and l-lysine-CM-cellulose are not inferior to the known l-lysine-sepharose. With electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate the plasminogen preparations obtained by the method of affined chromatography are homogeneous and their molecular mass is 87 000 Daltons.", "contents": "[Affinity sorbents for obtaining plasminogen]. Using immobilization of l-lysine on cellulose and CM-Cellulose activated with bromo-cyanogen and dicyclohexyl carboxyimide, respectively, the affined sorbents are obtained and possibility of their application for obtaining the high-purified plasminogen is studied. According to the yield and activity of plasminogen, l-lysine-cellulose and l-lysine-CM-cellulose are not inferior to the known l-lysine-sepharose. With electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate the plasminogen preparations obtained by the method of affined chromatography are homogeneous and their molecular mass is 87 000 Daltons."}
{"id": "PMID:473386", "title": "[Growth of rat fibroblasts in tissue culture as affected by trypsin immobilized on polyurethane substrates].", "content": "The growth of fibroblasts of the rat subcutaneous tissue was studied as affected by different doses of trypsin, free and bound, on polyurethane substrates. The method of tissue culture provides essential criteria for estimating the degree of bioincompatibility of polymeric alloimplants in particular when immobilizing biologically active substances on them. Trypsin adsorbed on the polyurethane substrate diffusing gradually into the culture medium is established to inhibit the cell growth. Chemically bounded trypsin in the composition of the polymeric matrix at the early stages of cultivation stimulates for a long time the division of fibroblastic elements and further accelerates their degeneration. It is shown that on polymeric substrates containing no trypsin, the growth character and dynamics of the fibroblastic elements are similar on the whole to these indices for cultures grown in the plasm clot without the substrate.", "contents": "[Growth of rat fibroblasts in tissue culture as affected by trypsin immobilized on polyurethane substrates]. The growth of fibroblasts of the rat subcutaneous tissue was studied as affected by different doses of trypsin, free and bound, on polyurethane substrates. The method of tissue culture provides essential criteria for estimating the degree of bioincompatibility of polymeric alloimplants in particular when immobilizing biologically active substances on them. Trypsin adsorbed on the polyurethane substrate diffusing gradually into the culture medium is established to inhibit the cell growth. Chemically bounded trypsin in the composition of the polymeric matrix at the early stages of cultivation stimulates for a long time the division of fibroblastic elements and further accelerates their degeneration. It is shown that on polymeric substrates containing no trypsin, the growth character and dynamics of the fibroblastic elements are similar on the whole to these indices for cultures grown in the plasm clot without the substrate."}
{"id": "PMID:473388", "title": "[Catalytic properties of immobilized cells of methane-oxidizing bacteria].", "content": "The article deals with conditions for immobilization of methane-oxidizing bacteria cells as well as with catalytic properties of the immobilized cells. The method of immobilization in polyacrylamide gel is shown to be not suitable for cells of methane-oxidizing bacteria. The greatest number of cells (85%) is immobilized on sylochrome modified by cyanuric chloride. However, the catalytic properties of the methane-oxidizing bacteria are better retained when the bacteria are immobilized on sylochrome modified by isocyanate. A stand installation is created for studying the catalytic properties of the immobilized cells oxidizing substrates in the gas phase.", "contents": "[Catalytic properties of immobilized cells of methane-oxidizing bacteria]. The article deals with conditions for immobilization of methane-oxidizing bacteria cells as well as with catalytic properties of the immobilized cells. The method of immobilization in polyacrylamide gel is shown to be not suitable for cells of methane-oxidizing bacteria. The greatest number of cells (85%) is immobilized on sylochrome modified by cyanuric chloride. However, the catalytic properties of the methane-oxidizing bacteria are better retained when the bacteria are immobilized on sylochrome modified by isocyanate. A stand installation is created for studying the catalytic properties of the immobilized cells oxidizing substrates in the gas phase."}
{"id": "PMID:473387", "title": "[Application of immobilized animal liver microsomal fraction to the reaction of 1,4-benzdiazepine-2-one conversion].", "content": "The albino rat strain Wistar and Shynashylla rabbit liver microsomal fraction was immobilized by incorporation into polyacrylamide gel. It is shown, that the microsomal fraction immobilized by such a method catalyzes the hydrolysis of 1,4-benzdiazepine-2 ones, 3-acetoxyderivatives, hydroxylation in position 3 of 1,4-benzdiazepine-2-ones and reduction of nitrazepame. The storage thermostability and stability of the immobilized preparations were studied. Immobilization of the animal liver microsomal fraction was conducted on sylochrome by cyanuric chloride and 2,4-toluylenediisocyanate.", "contents": "[Application of immobilized animal liver microsomal fraction to the reaction of 1,4-benzdiazepine-2-one conversion]. The albino rat strain Wistar and Shynashylla rabbit liver microsomal fraction was immobilized by incorporation into polyacrylamide gel. It is shown, that the microsomal fraction immobilized by such a method catalyzes the hydrolysis of 1,4-benzdiazepine-2 ones, 3-acetoxyderivatives, hydroxylation in position 3 of 1,4-benzdiazepine-2-ones and reduction of nitrazepame. The storage thermostability and stability of the immobilized preparations were studied. Immobilization of the animal liver microsomal fraction was conducted on sylochrome by cyanuric chloride and 2,4-toluylenediisocyanate."}
{"id": "PMID:473389", "title": "[Transformation of substrates not used for growth by the immobilized methane-oxidizing bacteria].", "content": "The article deals with the theoretical problems of obtaining certain substrates transformation products using immobilized cells of the methane-oxidizing bacteria. This process is based on the ability of these bacteria to cometabolize the substrates which are not used for growth. The methane-oxidizing bacteria are shown to utilize ethane during growth on methanol. Cometabolism of ethane and methanol occurs due to the presence of conjugated points of these substances oxidizing reactions as well as of the oxidation products assimilation reactions. The optimal concentrations of ethane and methanol are established for the methane-oxidizing bacteria growth and for formation of the ethane oxidation products by means of the immobilized cells.", "contents": "[Transformation of substrates not used for growth by the immobilized methane-oxidizing bacteria]. The article deals with the theoretical problems of obtaining certain substrates transformation products using immobilized cells of the methane-oxidizing bacteria. This process is based on the ability of these bacteria to cometabolize the substrates which are not used for growth. The methane-oxidizing bacteria are shown to utilize ethane during growth on methanol. Cometabolism of ethane and methanol occurs due to the presence of conjugated points of these substances oxidizing reactions as well as of the oxidation products assimilation reactions. The optimal concentrations of ethane and methanol are established for the methane-oxidizing bacteria growth and for formation of the ethane oxidation products by means of the immobilized cells."}
{"id": "PMID:473390", "title": "[Studies of sorption and interaction of chlorophyll and chlorophyllase on methylaerosil].", "content": "The article deals with the possibility of chlorophyll and chlorophyllase immobilization on methylaerosil and also with the effect of this carrier on the enzyme-substrate interaction. Chlorophyll and chlorophyllase are shown to be able to hydrophorbic interaction. It is established that the ultimate saturation of methylaerosil with chlorophyll is 16.1 mg per 100 mg of the carrier. The chlorophyllase reaction is the most active under conditions when the enzyme and substrate are in soluble states. The hydropholic binding of chlorophyll with methylaerosil decreases the intensity of its hydrolysis for the period of reaction and the preliminary immobilization of chlorophyllase on methylaerosil inhibits the reaction to a still greater extent. This evidences for the significance of the reaction system organization for interaction of chlorophyllase with chlorophyll.", "contents": "[Studies of sorption and interaction of chlorophyll and chlorophyllase on methylaerosil]. The article deals with the possibility of chlorophyll and chlorophyllase immobilization on methylaerosil and also with the effect of this carrier on the enzyme-substrate interaction. Chlorophyll and chlorophyllase are shown to be able to hydrophorbic interaction. It is established that the ultimate saturation of methylaerosil with chlorophyll is 16.1 mg per 100 mg of the carrier. The chlorophyllase reaction is the most active under conditions when the enzyme and substrate are in soluble states. The hydropholic binding of chlorophyll with methylaerosil decreases the intensity of its hydrolysis for the period of reaction and the preliminary immobilization of chlorophyllase on methylaerosil inhibits the reaction to a still greater extent. This evidences for the significance of the reaction system organization for interaction of chlorophyllase with chlorophyll."}
{"id": "PMID:473391", "title": "[Role of the molecular environment in chlorophyllase functioning during its immobilization on organic carriers].", "content": "Immobilization of chlorophyllase was performed on aminohexadecyl sepharose, aminoundecyl sepharose and heptyl sepharose. The enzyme activity lowers considerably and is rather close for all carriers. Essential differences are manifested in stability of the preparation during storage. On aminohexadecyl sepharose, the most hydrophobic carrier, the activity of chlorophyllase is 1.4 times as low for a month, on aminoundecyl sepharose for the same period it is 2.1 times as low and on heptyl sepharose--4.8 times as low. The covalent binding of chlorophyllase with sepharose activated by bromo-cyanogen with the subsequent fixation of diethyl amine or dodecyl amine possessing different hydrophobic properties showed that diethyl amine favours to a greater extent manifestation of the chlorophyllase activity and stabilization of the enzyme in time than dodecylamine. The data obtained evidence for a considerable role of the enzyme sstate and its molecular environment in manifestation of its functional activity and in providing its stability.", "contents": "[Role of the molecular environment in chlorophyllase functioning during its immobilization on organic carriers]. Immobilization of chlorophyllase was performed on aminohexadecyl sepharose, aminoundecyl sepharose and heptyl sepharose. The enzyme activity lowers considerably and is rather close for all carriers. Essential differences are manifested in stability of the preparation during storage. On aminohexadecyl sepharose, the most hydrophobic carrier, the activity of chlorophyllase is 1.4 times as low for a month, on aminoundecyl sepharose for the same period it is 2.1 times as low and on heptyl sepharose--4.8 times as low. The covalent binding of chlorophyllase with sepharose activated by bromo-cyanogen with the subsequent fixation of diethyl amine or dodecyl amine possessing different hydrophobic properties showed that diethyl amine favours to a greater extent manifestation of the chlorophyllase activity and stabilization of the enzyme in time than dodecylamine. The data obtained evidence for a considerable role of the enzyme sstate and its molecular environment in manifestation of its functional activity and in providing its stability."}
{"id": "PMID:473392", "title": "[Prospects of applying immobilized enzymes in medicine].", "content": "The paper deals with evidence on main trends for using immobilized enzymes in medicine. The lyposomes are shown to be means for delivering enzymes to a cell under certain hereditary diseases. The prospects are considered for using the bound enzymes for treating some pathologic processes (py0-inflammatory, thrombosis, malignant tumors, etc.). The data are presented on possibilities of applying the immobilized enzymes as specific reagents for determining substrates and metabolites in the disease diagnostics.", "contents": "[Prospects of applying immobilized enzymes in medicine]. The paper deals with evidence on main trends for using immobilized enzymes in medicine. The lyposomes are shown to be means for delivering enzymes to a cell under certain hereditary diseases. The prospects are considered for using the bound enzymes for treating some pathologic processes (py0-inflammatory, thrombosis, malignant tumors, etc.). The data are presented on possibilities of applying the immobilized enzymes as specific reagents for determining substrates and metabolites in the disease diagnostics."}
{"id": "PMID:473393", "title": "[Age peculiarities in the content of nucleic acids, collagen (as oxyproline) and other proteins in rat skin and lung tissues].", "content": "It is established that in old animals, as compared to the young ones, the content of nucleic acids, the total content of protein and the content of the peptide-bound oxyproline in the collagen soluble fractions in the skin and lungs tissues decrease, whereas the content of peptide-bound oxyproline in the insoluble collagen and of free oxyproline, vice versa, increases.", "contents": "[Age peculiarities in the content of nucleic acids, collagen (as oxyproline) and other proteins in rat skin and lung tissues]. It is established that in old animals, as compared to the young ones, the content of nucleic acids, the total content of protein and the content of the peptide-bound oxyproline in the collagen soluble fractions in the skin and lungs tissues decrease, whereas the content of peptide-bound oxyproline in the insoluble collagen and of free oxyproline, vice versa, increases."}
{"id": "PMID:473394", "title": "[Physiocochemical properties of blood serum proteins of coal miners].", "content": "Using disk electrophoresis in the polyacrylamide gel, blood serum proteins were studied in miners working under conditions of the combine (the control group) and drilling-and-blasting (the contact with carbon oxide, nitrogen oxides) driving technique under normal temperature conditions. 26--27 protein fractions characterized by mobility, thermolability under definite conditions of the experiment and the contitative content were obtained. It is shown that the contact with carbon oxide and nitrogen oxides causes changes in the rpoperties of certain proteins (II3, globulins--2 alpha 1, 3 alpha 1, 2 beta, 2 alpha 2, 5 alpha 2, 6 alpha 2, 7 alpha 2) of miners blood serum. Some of these proteins are supposed to participate in the adaptation reactions of the organism.", "contents": "[Physiocochemical properties of blood serum proteins of coal miners]. Using disk electrophoresis in the polyacrylamide gel, blood serum proteins were studied in miners working under conditions of the combine (the control group) and drilling-and-blasting (the contact with carbon oxide, nitrogen oxides) driving technique under normal temperature conditions. 26--27 protein fractions characterized by mobility, thermolability under definite conditions of the experiment and the contitative content were obtained. It is shown that the contact with carbon oxide and nitrogen oxides causes changes in the rpoperties of certain proteins (II3, globulins--2 alpha 1, 3 alpha 1, 2 beta, 2 alpha 2, 5 alpha 2, 6 alpha 2, 7 alpha 2) of miners blood serum. Some of these proteins are supposed to participate in the adaptation reactions of the organism."}
{"id": "PMID:473395", "title": "[Quantitative method of estimating the conformation changes in blood serum albumin].", "content": "The method of residementation with trichloracetic acid followed by dissolution in ethanol of preparations of the human serum albumin with changed conformation under conditions of pathology was suggested for determining the amount of modified albumin. The conformationally changed albumin is separated by the mentioned method and it changes to the insoluble form. According to the content of albumin in the sediment it is possible to estimate the amount of the modified part of this protein which was determined previously by the labour-consuming method of optical rotation dispersion.", "contents": "[Quantitative method of estimating the conformation changes in blood serum albumin]. The method of residementation with trichloracetic acid followed by dissolution in ethanol of preparations of the human serum albumin with changed conformation under conditions of pathology was suggested for determining the amount of modified albumin. The conformationally changed albumin is separated by the mentioned method and it changes to the insoluble form. According to the content of albumin in the sediment it is possible to estimate the amount of the modified part of this protein which was determined previously by the labour-consuming method of optical rotation dispersion."}
{"id": "PMID:473421", "title": "Radiation damage of purple membrane at low temperature.", "content": "The intensities of diffracted electron beams for the purple membrane of Halobacterium halobium are found to decay exponentially as a function of the accumulated electron exposure, both at room temperature and at -120 degrees C. This permits us to define the \"critical dose\" Ne(h,k) for the (h,k) diffracted beam, as being the electron exposure (electrons/A2) at which the diffracteed intensity has fallen to e-1 of its initial value. The critical of purple membrane is found to increase from the room temperature value by at least a factor of four when the specimen is maintained at a temperature of -120 degrees C on a liquid-nitrogen-cooled stage. A relationship derived between the critical dose, Ne, and the dose for optimum imaging, Nopt. Both Ne and Nopt depend, of course, upon the spatial frequency, or resolution. The derivation is valid only for the case in which all sources of noise other than quantum fluctuations are neglected. In this case, Nopt approximately equal to 2.5Ne. Finally, Nuclear Track Emulsion plates have been shown to be advantageous for recording high resolution electron diffraction patterns of small (1 micrometer 2) patches of crystalline biological materials.", "contents": "Radiation damage of purple membrane at low temperature. The intensities of diffracted electron beams for the purple membrane of Halobacterium halobium are found to decay exponentially as a function of the accumulated electron exposure, both at room temperature and at -120 degrees C. This permits us to define the \"critical dose\" Ne(h,k) for the (h,k) diffracted beam, as being the electron exposure (electrons/A2) at which the diffracteed intensity has fallen to e-1 of its initial value. The critical of purple membrane is found to increase from the room temperature value by at least a factor of four when the specimen is maintained at a temperature of -120 degrees C on a liquid-nitrogen-cooled stage. A relationship derived between the critical dose, Ne, and the dose for optimum imaging, Nopt. Both Ne and Nopt depend, of course, upon the spatial frequency, or resolution. The derivation is valid only for the case in which all sources of noise other than quantum fluctuations are neglected. In this case, Nopt approximately equal to 2.5Ne. Finally, Nuclear Track Emulsion plates have been shown to be advantageous for recording high resolution electron diffraction patterns of small (1 micrometer 2) patches of crystalline biological materials."}
{"id": "PMID:473422", "title": "Ultrasonic pulsed Doppler transoesophageal measurement of aortic haemodynamics in humans.", "content": "Centreline blood velocity and exterior wall motion were measured in the descending aorta of humans using an oesophageal probe, a pulsed ultrasound Doppler velocity meter and an ultrasonic echo tracking system. The development of a method for easily measuring haemodynamics in the thoracic region using an oesophageal probe will provide an essentially non-invasive method for the assessment of cardiac function and the nature of blood vessels in that region. Detailed anatomical studies of the thorax were conducted by cross-sectioning of the thorax of a cadaver. Blood velocity waveforms were recorded from the descending aorta both during rest and exercise. In one volunteer, the peak centreline velocity increased from a resting value of approximately 30 cm s-1 to an exercise value of approximately 50 cm s-1. Vessel diameter waveforms similar to those for pressure were also recorded showing diameter changes of 1.8 mm. The accuracy and resolution of the technique would be improved by multicrystal probes and multigate ultrasonic flowmeters allowing for accurate calculation of the Doppler angle, imaging of vascular flow regions, and measurement of pulse wave velocity.", "contents": "Ultrasonic pulsed Doppler transoesophageal measurement of aortic haemodynamics in humans. Centreline blood velocity and exterior wall motion were measured in the descending aorta of humans using an oesophageal probe, a pulsed ultrasound Doppler velocity meter and an ultrasonic echo tracking system. The development of a method for easily measuring haemodynamics in the thoracic region using an oesophageal probe will provide an essentially non-invasive method for the assessment of cardiac function and the nature of blood vessels in that region. Detailed anatomical studies of the thorax were conducted by cross-sectioning of the thorax of a cadaver. Blood velocity waveforms were recorded from the descending aorta both during rest and exercise. In one volunteer, the peak centreline velocity increased from a resting value of approximately 30 cm s-1 to an exercise value of approximately 50 cm s-1. Vessel diameter waveforms similar to those for pressure were also recorded showing diameter changes of 1.8 mm. The accuracy and resolution of the technique would be improved by multicrystal probes and multigate ultrasonic flowmeters allowing for accurate calculation of the Doppler angle, imaging of vascular flow regions, and measurement of pulse wave velocity."}
{"id": "PMID:473423", "title": "An investigation of a spread energy method for medical ultrasound systems. Part two: proposed system and possible problems.", "content": "A medical ultrasound device using a Golay code is proposed and investigated theoretically. The code enables the spread energy method to be used to help overcome the restraints of the parameters of resolution, peak power and penetration.", "contents": "An investigation of a spread energy method for medical ultrasound systems. Part two: proposed system and possible problems. A medical ultrasound device using a Golay code is proposed and investigated theoretically. The code enables the spread energy method to be used to help overcome the restraints of the parameters of resolution, peak power and penetration."}
{"id": "PMID:473424", "title": "A new delay technique with application to ultrasound phased-array imaging systems.", "content": "In ultrasound phased-array imaging systems the receiver directivity pattern is steered by controlling the delay of echoes from the array elements. A new processing technique is introduced that provides the required delay first by properly phasing the echo carrier, and successively by delaying the echo envelope. This latter operation is based on a new analogue sampled-data delay-line. Independent control of phase delay offers a simple means of compensating for random phase errors due to non-uniformity of the array elements response.", "contents": "A new delay technique with application to ultrasound phased-array imaging systems. In ultrasound phased-array imaging systems the receiver directivity pattern is steered by controlling the delay of echoes from the array elements. A new processing technique is introduced that provides the required delay first by properly phasing the echo carrier, and successively by delaying the echo envelope. This latter operation is based on a new analogue sampled-data delay-line. Independent control of phase delay offers a simple means of compensating for random phase errors due to non-uniformity of the array elements response."}
{"id": "PMID:473426", "title": "Urethral pressure profile and retrograde urethrography in diagnosis of urethral strictures.", "content": "In 20 male patients with urethral strictures, urethral pressure profile was compared with retrograde urethrography. There was good accordance between the two methods with only minor discrepancies in multiple or very distal strictures. Urethral profile did not provide more exact information than did urethrography.", "contents": "Urethral pressure profile and retrograde urethrography in diagnosis of urethral strictures. In 20 male patients with urethral strictures, urethral pressure profile was compared with retrograde urethrography. There was good accordance between the two methods with only minor discrepancies in multiple or very distal strictures. Urethral profile did not provide more exact information than did urethrography."}
{"id": "PMID:473427", "title": "Comparative analysis of the results of suspension-urethroplasty according to Marshall-Marchetti-Krantz and of urethrovesicopexy with adhesive.", "content": "A comparative analysis is presented of the results of adhesive urethrovesicopexy and the conventional suspension method according to Marshall, Marchetti and Krantz. It appears that the results are less favorable when compared to those mentioned in the literature, and no doubt attributable to the difficult differential diagnosis of stress and urge incontinence. The adhesive method supplies significantly less favorable results than the conventional method according to Marshall, Marchetti and Krantz.", "contents": "Comparative analysis of the results of suspension-urethroplasty according to Marshall-Marchetti-Krantz and of urethrovesicopexy with adhesive. A comparative analysis is presented of the results of adhesive urethrovesicopexy and the conventional suspension method according to Marshall, Marchetti and Krantz. It appears that the results are less favorable when compared to those mentioned in the literature, and no doubt attributable to the difficult differential diagnosis of stress and urge incontinence. The adhesive method supplies significantly less favorable results than the conventional method according to Marshall, Marchetti and Krantz."}
{"id": "PMID:473428", "title": "Functional abnormality of 'non-provocative' bladder instability in children.", "content": "In the last years the symptom of unstable bladder behaviour has been interpreted with increasing clinical importance, sometimes being regarded as a diagnosis or as a causative factor for different complaints (1, 3-6, 16, 18, 20). The term 'unstable bladder' is still not precisely defined (17). Few investigations on this topic had been done in children, therefore, the urodynamic findings of 216 children were analysed to look for co-findings of bladder instability and, if possible, to demonstrate some causative factors. The investigations revealed that unstable bladder behaviour seems to be a secondary cystometric phenomenon (directly or indirectly induced). The treatment of unstable bladder should be concentrated on the causative disorder rather than on the cystometric symptom", "contents": "Functional abnormality of 'non-provocative' bladder instability in children. In the last years the symptom of unstable bladder behaviour has been interpreted with increasing clinical importance, sometimes being regarded as a diagnosis or as a causative factor for different complaints (1, 3-6, 16, 18, 20). The term 'unstable bladder' is still not precisely defined (17). Few investigations on this topic had been done in children, therefore, the urodynamic findings of 216 children were analysed to look for co-findings of bladder instability and, if possible, to demonstrate some causative factors. The investigations revealed that unstable bladder behaviour seems to be a secondary cystometric phenomenon (directly or indirectly induced). The treatment of unstable bladder should be concentrated on the causative disorder rather than on the cystometric symptom"}
{"id": "PMID:473429", "title": "Serum xanthine oxidase in bladder carcinoma.", "content": "This study comprises of 48 normal men as control group and 49 patients with various stages of bladder carcinoma. Serum xanthine oxidase and uric acid levels were determined. Assessment in terms of changes of the enzyme xanthine oxidase was carried out prior and after surgery as a short-term follow-up. There was a significant fall of serum xanthine oxidase in patients with bladder carcinoma; it also varied with the stage of cancer.", "contents": "Serum xanthine oxidase in bladder carcinoma. This study comprises of 48 normal men as control group and 49 patients with various stages of bladder carcinoma. Serum xanthine oxidase and uric acid levels were determined. Assessment in terms of changes of the enzyme xanthine oxidase was carried out prior and after surgery as a short-term follow-up. There was a significant fall of serum xanthine oxidase in patients with bladder carcinoma; it also varied with the stage of cancer."}
{"id": "PMID:473430", "title": "[Desobstructive sodium and water diuresis: pathophysiologic and clinical aspects of bilateral obstructive nephropathy (author's transl)].", "content": "The measurement of sodium and water loss after relieve of the obstruction shows that the obstructive nephropathy originates in three pathogenetic mechanisms. (1) Acute complete bilateral ureteric obstruction causes tubular atrophy, prevents both resorption and glomerular filtration, reduces the renal blood flow and increases the extracellular space by retention of water and products subject to urinary excretion. (2) Relieve of obstruction results in excess polyuria as blood flow and glomerular filtration recover rapidly, the extracellular space gets rid of its osmotic load, and the tubular dysfunction of resorption continues for several days until the epithelium has recovered from its pressure atrophy. All this will result in a high loss of sodium and water which requires adequate substitution; otherwise, natriuretic shock will result. (3) The chronic (bilateral) obstruction behaves in a similar way, yet is less reversible. The tubular damage is the same. Moderate polyuria occurs already during the stage of obstruction. Hereby the extracellular space decreases. After relieve of the obstruction the polyuria increases significantly, yet less rapidly than after acute obstruction as the glomerular function does not recover completely. The renal blood flow remains diminished, the vascular calibers stay narrowed, and the kidney remains shrunken. Loss of sodium and water will endanger the patient with chronic obstruction. Furthermore, the patient will be at risk due to dehydration, acidosis, anemia and uremia. The infusion therapy of the desobstructive nephropathy syndrome is based upon the venous pressure and the serum electrolytes which are measured twice daily.", "contents": "[Desobstructive sodium and water diuresis: pathophysiologic and clinical aspects of bilateral obstructive nephropathy (author's transl)]. The measurement of sodium and water loss after relieve of the obstruction shows that the obstructive nephropathy originates in three pathogenetic mechanisms. (1) Acute complete bilateral ureteric obstruction causes tubular atrophy, prevents both resorption and glomerular filtration, reduces the renal blood flow and increases the extracellular space by retention of water and products subject to urinary excretion. (2) Relieve of obstruction results in excess polyuria as blood flow and glomerular filtration recover rapidly, the extracellular space gets rid of its osmotic load, and the tubular dysfunction of resorption continues for several days until the epithelium has recovered from its pressure atrophy. All this will result in a high loss of sodium and water which requires adequate substitution; otherwise, natriuretic shock will result. (3) The chronic (bilateral) obstruction behaves in a similar way, yet is less reversible. The tubular damage is the same. Moderate polyuria occurs already during the stage of obstruction. Hereby the extracellular space decreases. After relieve of the obstruction the polyuria increases significantly, yet less rapidly than after acute obstruction as the glomerular function does not recover completely. The renal blood flow remains diminished, the vascular calibers stay narrowed, and the kidney remains shrunken. Loss of sodium and water will endanger the patient with chronic obstruction. Furthermore, the patient will be at risk due to dehydration, acidosis, anemia and uremia. The infusion therapy of the desobstructive nephropathy syndrome is based upon the venous pressure and the serum electrolytes which are measured twice daily."}
{"id": "PMID:473431", "title": "Indication and value of bladder neck incision.", "content": "200 bladder neck incisions in 172 patients with mechanical or functional outlet obstruction of neurogenic and nonneurogenic etiology are reported. The incision was performed with an electric cautery needle at 5--7--12 o'clock. Parameters of success were, beside the subjective reports, residual urine, urinary flow and a sophisticated urodynamic study. 71% of the women and 76% of the men showed improvement or relief of symptoms. Retrograde ejaculation was observed in only 7% of the men; the rate of complications was 2.5%. Despite limited experience and the lack of long-term follow-up, this technique is recommended for routine use.", "contents": "Indication and value of bladder neck incision. 200 bladder neck incisions in 172 patients with mechanical or functional outlet obstruction of neurogenic and nonneurogenic etiology are reported. The incision was performed with an electric cautery needle at 5--7--12 o'clock. Parameters of success were, beside the subjective reports, residual urine, urinary flow and a sophisticated urodynamic study. 71% of the women and 76% of the men showed improvement or relief of symptoms. Retrograde ejaculation was observed in only 7% of the men; the rate of complications was 2.5%. Despite limited experience and the lack of long-term follow-up, this technique is recommended for routine use."}
{"id": "PMID:473443", "title": "Epidemiological study of 314 cases of cancer of the bladder in the administrative department of Bas-Rhin, France.", "content": "This study is composed of two epidemiological investigations. The first concerns the geographical distribution of cancer of the bladder in the department of Bas-Rhin. The geographical distribution of 314 cases of cancer of the bladder in eight districts of the Bas-Rhin has been studied. The resultant map shows considerable variations in morbidity. The urban predominance may be due to environmental factors. The second enquiry concerns the frequency of multiple cancers of different primary sites occurring in cases of cancer of the bladder. Nine such cases have been identified. There may be a common predisposing factor in such cases. All patients were heavy smokers.", "contents": "Epidemiological study of 314 cases of cancer of the bladder in the administrative department of Bas-Rhin, France. This study is composed of two epidemiological investigations. The first concerns the geographical distribution of cancer of the bladder in the department of Bas-Rhin. The geographical distribution of 314 cases of cancer of the bladder in eight districts of the Bas-Rhin has been studied. The resultant map shows considerable variations in morbidity. The urban predominance may be due to environmental factors. The second enquiry concerns the frequency of multiple cancers of different primary sites occurring in cases of cancer of the bladder. Nine such cases have been identified. There may be a common predisposing factor in such cases. All patients were heavy smokers."}
{"id": "PMID:473445", "title": "Effect of intralesional and systemic BCG-application or a combined cyclophosphamide/BCG treatment on experimental bladder cancer.", "content": "Infiltrating transitional cell carcinomas of the urinary bladder were induced by ingestion of 0.188% N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide (FANFT) in 145 female Wistar rats. After 8 months of carcinogen exposure, the animals were divided into different treatment groups. They received cyclophosphamide intraperitoneally as a single injection or BCG either once intralesionally or weekly subcutaneously or a combination of cyclophosphamide followed by subcutaneous BCG. The treatment effect was determined by body weight measurements and bladder tumour weight after 12 months. Compared with a control group statistically significant differences of bladder tumour weights were found after treatment with BCG alone or in combination with cyclophosphamide. Intralesional BCG resulted in an insignificant increase of tumour weights.", "contents": "Effect of intralesional and systemic BCG-application or a combined cyclophosphamide/BCG treatment on experimental bladder cancer. Infiltrating transitional cell carcinomas of the urinary bladder were induced by ingestion of 0.188% N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide (FANFT) in 145 female Wistar rats. After 8 months of carcinogen exposure, the animals were divided into different treatment groups. They received cyclophosphamide intraperitoneally as a single injection or BCG either once intralesionally or weekly subcutaneously or a combination of cyclophosphamide followed by subcutaneous BCG. The treatment effect was determined by body weight measurements and bladder tumour weight after 12 months. Compared with a control group statistically significant differences of bladder tumour weights were found after treatment with BCG alone or in combination with cyclophosphamide. Intralesional BCG resulted in an insignificant increase of tumour weights."}
{"id": "PMID:473446", "title": "Post-irradiation total cystectomy for bladder cancer.", "content": "Treatment with radiotherapy (6000 rad) followed by total cystectomy was carried out in 125 patients with T1-T3 bladder cancer, the tumour being poorly differentiated (grade III-IV) in 115 cases. Survival was significantly better, if the irradiation had resulted in complete tumour regression before cystectomy, and if the tumour at the initial examination was of category T1. The relative cumulative survival after 5 years for patients with T1-tumours was 60%, as opposed to 40% for patients with T3-tumours. 4 patients (2.4%) died within 30 days of the cystectomy, but 13 patients (10%) died within 3 years from complications of the treatment regime. 83 patients (66%) experienced non-fatal complications such as infected cavity and urinary or intestinal stenosis. 3 years after the cystectomy 62 patients (50%) were dead from recurrent tumour or from other causes.", "contents": "Post-irradiation total cystectomy for bladder cancer. Treatment with radiotherapy (6000 rad) followed by total cystectomy was carried out in 125 patients with T1-T3 bladder cancer, the tumour being poorly differentiated (grade III-IV) in 115 cases. Survival was significantly better, if the irradiation had resulted in complete tumour regression before cystectomy, and if the tumour at the initial examination was of category T1. The relative cumulative survival after 5 years for patients with T1-tumours was 60%, as opposed to 40% for patients with T3-tumours. 4 patients (2.4%) died within 30 days of the cystectomy, but 13 patients (10%) died within 3 years from complications of the treatment regime. 83 patients (66%) experienced non-fatal complications such as infected cavity and urinary or intestinal stenosis. 3 years after the cystectomy 62 patients (50%) were dead from recurrent tumour or from other causes."}
{"id": "PMID:473447", "title": "Grading of transitional cell tumours of the urinary tract by urinary cytology.", "content": "1122 cytological diagnoses have been made on 2500 Papanicolaou stained cytological slides from Patients with urothelial carcinomas. The cytological accuracy was 78% for G1 tumours, 94% for G2 and 96% for G3 carcinomas. A histologically diagnosed G1 carcinoma was cytologically G1 in 81% of cases, a G2 carcinoma was recognized in 64% and G3 carcinoma in 48%.", "contents": "Grading of transitional cell tumours of the urinary tract by urinary cytology. 1122 cytological diagnoses have been made on 2500 Papanicolaou stained cytological slides from Patients with urothelial carcinomas. The cytological accuracy was 78% for G1 tumours, 94% for G2 and 96% for G3 carcinomas. A histologically diagnosed G1 carcinoma was cytologically G1 in 81% of cases, a G2 carcinoma was recognized in 64% and G3 carcinoma in 48%."}
{"id": "PMID:473448", "title": "Reliability of lymphography as a diagnostic aid in the planning of treatment for bladder carcinoma.", "content": "To assess the accuracy of lymphography, 90 lymphograms were compared with surgical and histological findings. Histological correlation with the initial report was found in 65 to 90 cases (72%). Comparison of the original radiologist's report with a recent re-examination showed agreement between the two radiological evaluations in only 74%. Although improvement in diagnostic accuracy can perhaps be obtained, we cannot yet recommend lymphography as an aid in the planning of treatment for bladder carcinoma.", "contents": "Reliability of lymphography as a diagnostic aid in the planning of treatment for bladder carcinoma. To assess the accuracy of lymphography, 90 lymphograms were compared with surgical and histological findings. Histological correlation with the initial report was found in 65 to 90 cases (72%). Comparison of the original radiologist's report with a recent re-examination showed agreement between the two radiological evaluations in only 74%. Although improvement in diagnostic accuracy can perhaps be obtained, we cannot yet recommend lymphography as an aid in the planning of treatment for bladder carcinoma."}
{"id": "PMID:473449", "title": "Prognostic significance of peritumoural inflammation in invasive urothelial bladder carcinoma.", "content": "Peritumoural inflammation consisting of lymphocytes, plasma cells and lymph follicles is present in two thirds of invasive urothelial bladder carcinomas. This type of inflammation is significantly rarer in advanced tumour stages (P3, P/) and its presence is a favourable prognostic factor. The mean survival time in patients with this type of inflammation is 26.1 months compared to only 14.8 months in patients without. All other forms of inflammation e. g. eosinophilic or polymorphonuclear leukocytes have no relation with prognosis. The significant prognostic value of lympho-plasmocytic inflammation may be considered as local expression of immunological host resistance.", "contents": "Prognostic significance of peritumoural inflammation in invasive urothelial bladder carcinoma. Peritumoural inflammation consisting of lymphocytes, plasma cells and lymph follicles is present in two thirds of invasive urothelial bladder carcinomas. This type of inflammation is significantly rarer in advanced tumour stages (P3, P/) and its presence is a favourable prognostic factor. The mean survival time in patients with this type of inflammation is 26.1 months compared to only 14.8 months in patients without. All other forms of inflammation e. g. eosinophilic or polymorphonuclear leukocytes have no relation with prognosis. The significant prognostic value of lympho-plasmocytic inflammation may be considered as local expression of immunological host resistance."}
{"id": "PMID:473450", "title": "[Improvement of male fertility through ligation of varicocele? A study of 100 subfertile varicocele patients three months after ligation (author's transl)].", "content": "With 100 subfertile varicocele patients, three months after ligation (high ligation of the left internal spermatic vein in accordance with Bernardi) had control spermiograms made. An improvement of sperm count (upgrade in a higher class according to MacLeod's partition) was found in 28% of the cases, whereas an improvement of progressive motility was found only in 13%. The percentage of patients reaching the normal value in sperm count of over 40 mill./ml increased from 23% to 39%. The increase in these patients came primarily from the class of 20--40 mill./ml. In progressive motility, there was an increase of patients reaching the standard value of over 40%, from 6 to 11. However, if sperm count and progressive motility together were evaluated in the individual patients after ligation, only 6 patients presented a normospermia, i.e. a sperm count of more than 40 mill./ml with normal progressive motility. Thus 94 of the 100 patients must be considered as subfertile three months after ligation. A significant fertility improvement through ligation of varicocele has not yet taken place.", "contents": "[Improvement of male fertility through ligation of varicocele? A study of 100 subfertile varicocele patients three months after ligation (author's transl)]. With 100 subfertile varicocele patients, three months after ligation (high ligation of the left internal spermatic vein in accordance with Bernardi) had control spermiograms made. An improvement of sperm count (upgrade in a higher class according to MacLeod's partition) was found in 28% of the cases, whereas an improvement of progressive motility was found only in 13%. The percentage of patients reaching the normal value in sperm count of over 40 mill./ml increased from 23% to 39%. The increase in these patients came primarily from the class of 20--40 mill./ml. In progressive motility, there was an increase of patients reaching the standard value of over 40%, from 6 to 11. However, if sperm count and progressive motility together were evaluated in the individual patients after ligation, only 6 patients presented a normospermia, i.e. a sperm count of more than 40 mill./ml with normal progressive motility. Thus 94 of the 100 patients must be considered as subfertile three months after ligation. A significant fertility improvement through ligation of varicocele has not yet taken place."}
{"id": "PMID:473451", "title": "[Pathogenesis and therapy of varicocele (author's transl)].", "content": "During the last 13 years 519 patients were operated upon for varicocele in the refertilisation center Hamburg. Out of 209 cases examined thereafter, 174 showed an improved spermiogram, and only in this group 40 children were fathered. Pathogenesis, diagnosis and surgical treatment are presented. A persisting varicocele (in our clientele in 1 per cent) was corrected in 18 patients. The reasons for recurrent surgery were overlooked branches of the V. spermatica interna and not yet described collaterals to neighbouring venous systems. For optimal surgical planning the latter are best demonstrated by preoperative phlebography.", "contents": "[Pathogenesis and therapy of varicocele (author's transl)]. During the last 13 years 519 patients were operated upon for varicocele in the refertilisation center Hamburg. Out of 209 cases examined thereafter, 174 showed an improved spermiogram, and only in this group 40 children were fathered. Pathogenesis, diagnosis and surgical treatment are presented. A persisting varicocele (in our clientele in 1 per cent) was corrected in 18 patients. The reasons for recurrent surgery were overlooked branches of the V. spermatica interna and not yet described collaterals to neighbouring venous systems. For optimal surgical planning the latter are best demonstrated by preoperative phlebography."}
{"id": "PMID:473452", "title": "[The conservative therapy of urolithiasis (author's transl)].", "content": "Some common conservative treating methods of urolithiasis are critically discussed. Based on physico-chemical relationships of solubility and cristallisation of stone-forming compounds our own methods of conservative treatment of urolithiasis is presented.", "contents": "[The conservative therapy of urolithiasis (author's transl)]. Some common conservative treating methods of urolithiasis are critically discussed. Based on physico-chemical relationships of solubility and cristallisation of stone-forming compounds our own methods of conservative treatment of urolithiasis is presented."}
{"id": "PMID:473453", "title": "[Potency disturbances following saphenocavernous bypass in priapism (Grayhack procedure) (author's transl)].", "content": "Five patients with priapism operated by the Grayhack procedure are presented disturbances were explored. Two of these patients having no fibrosis of the cavernous bodies had total impotence 8 months and 14 months after the operation. The cavernosogram revealed a patent shunt. Potency returned after ligation and division of the saphenous vein. In the other cases cavernosogram revealed normal venous return and thrombosis of the shunt. Fibrosis of the cavernous tissues was here the cause of the impotence. In all cases where erection does not occur within 3 months of the Grayhack procedure the shunt should be ligated.", "contents": "[Potency disturbances following saphenocavernous bypass in priapism (Grayhack procedure) (author's transl)]. Five patients with priapism operated by the Grayhack procedure are presented disturbances were explored. Two of these patients having no fibrosis of the cavernous bodies had total impotence 8 months and 14 months after the operation. The cavernosogram revealed a patent shunt. Potency returned after ligation and division of the saphenous vein. In the other cases cavernosogram revealed normal venous return and thrombosis of the shunt. Fibrosis of the cavernous tissues was here the cause of the impotence. In all cases where erection does not occur within 3 months of the Grayhack procedure the shunt should be ligated."}
{"id": "PMID:473454", "title": "[Obstructive aspermia. successful treatment by transurethral resection of the verumontanum. A case report (author's transl)].", "content": "After a barren marriage for 2 1/2 years the 22 years old male patient presented for consultation. The examinations confirmed obstructive aspermia with acceptable spermatogenesis. The site of obstruction involved the colliculus seminalis. Transurethral resection of the colliculus was performed. Following a protracted postoperative andrological treatment pregnancy ensued with uncomplicated delivery.", "contents": "[Obstructive aspermia. successful treatment by transurethral resection of the verumontanum. A case report (author's transl)]. After a barren marriage for 2 1/2 years the 22 years old male patient presented for consultation. The examinations confirmed obstructive aspermia with acceptable spermatogenesis. The site of obstruction involved the colliculus seminalis. Transurethral resection of the colliculus was performed. Following a protracted postoperative andrological treatment pregnancy ensued with uncomplicated delivery."}
{"id": "PMID:473460", "title": "Useful technique for long-term urinary drainage by inlying ureteral stent. Six-year experience.", "content": "Endoscopically placed inlying ureteral stents have proved useful in the conservative management of patients with ureteral obstruction, urinary fistula, and malignancy and have obviated the need for operative intervention. In high-risk symptomatic patients with widespread malignancy, internal urinary diversion offers the opportunity for an improved quality of life without the surgical risk or potential morbidity of supravesical diversion. Potential candidates for this simple, safe, and effective technique include: those with postsurgical obstruction and/or fistula, retroperitoneal fibrosis, metastatic carcinoma, congenital ureteropelvic junction obstruction, as well as those with reversible obstruction from lymphoma and carcinoma of the prostate who are undergoing radiotherapy and/or chemotherapy. The focus of this report is on the technique we have found successful in providing us with stents that fit our individual patients. Readily available fabricated graduated ureteral catheter can be cut and shaped to particular measurements unlike prefabricated catheters. Minimal preparation time is demanded, and there is no need for extensive stocking of various catheter sizes.", "contents": "Useful technique for long-term urinary drainage by inlying ureteral stent. Six-year experience. Endoscopically placed inlying ureteral stents have proved useful in the conservative management of patients with ureteral obstruction, urinary fistula, and malignancy and have obviated the need for operative intervention. In high-risk symptomatic patients with widespread malignancy, internal urinary diversion offers the opportunity for an improved quality of life without the surgical risk or potential morbidity of supravesical diversion. Potential candidates for this simple, safe, and effective technique include: those with postsurgical obstruction and/or fistula, retroperitoneal fibrosis, metastatic carcinoma, congenital ureteropelvic junction obstruction, as well as those with reversible obstruction from lymphoma and carcinoma of the prostate who are undergoing radiotherapy and/or chemotherapy. The focus of this report is on the technique we have found successful in providing us with stents that fit our individual patients. Readily available fabricated graduated ureteral catheter can be cut and shaped to particular measurements unlike prefabricated catheters. Minimal preparation time is demanded, and there is no need for extensive stocking of various catheter sizes."}
{"id": "PMID:473461", "title": "Revascularization of corpus cavernosum for erectile failure.", "content": "Revascularization of the penis for erectile disability was performed in 10 patients in whom arterial obstruction had been documented. Inferior epigastric artery anastomosis to the corpus cavernosum was not successful, although bypass grafts from the femoral artery to the corpus cavernosum have helped restore potency in 4 of 6 patients. Complications included embolus to the leg, postoperative hemorrhage, and three small hematomas. This procedure can be considered a clinical experiment and would call for more study to assess its ultimate value.", "contents": "Revascularization of corpus cavernosum for erectile failure. Revascularization of the penis for erectile disability was performed in 10 patients in whom arterial obstruction had been documented. Inferior epigastric artery anastomosis to the corpus cavernosum was not successful, although bypass grafts from the femoral artery to the corpus cavernosum have helped restore potency in 4 of 6 patients. Complications included embolus to the leg, postoperative hemorrhage, and three small hematomas. This procedure can be considered a clinical experiment and would call for more study to assess its ultimate value."}
{"id": "PMID:473462", "title": "Simplified coagulum pyelolithotomy using cryoprecipitate.", "content": "Coagulum pyelolithotomy has been beneficial in removing calyceal calculi when the diameter of the infundibulum and pelvis is adequate. By utilizing a simple, precise ratio of cryoprecipitate, thrombin, and calcium chloride (25:4:1), an effective coagulum was obtained in 7 consecutive cases with complete stone removal in 5.", "contents": "Simplified coagulum pyelolithotomy using cryoprecipitate. Coagulum pyelolithotomy has been beneficial in removing calyceal calculi when the diameter of the infundibulum and pelvis is adequate. By utilizing a simple, precise ratio of cryoprecipitate, thrombin, and calcium chloride (25:4:1), an effective coagulum was obtained in 7 consecutive cases with complete stone removal in 5."}
{"id": "PMID:473463", "title": "Quantitative radiology for staging and prognosis of patients with advanced prostatic carcinoma. Correlations with other pretreatment characteristics.", "content": "Quantitative radiology of metastases is compared with other pretreatment characteristics in ability to predict survival in 102 patients with advanced Stage IV prostatic carcinoma. Using only two films, the posteroanterior chest and pelvis, radiologic quantitation of metastases is more precise than the other pretreatment characteristics in separating high-risk and low-risk patients. Quantitative radiology is a simple, noninvasive, effective method of stratifying patients in clinical trials or before aggressive therapy. The comparative ability of quantitative radiology and quantitative bone scans to separate high-risk and low-risk patients with advanced prostatic carcinoma can now be assessed.", "contents": "Quantitative radiology for staging and prognosis of patients with advanced prostatic carcinoma. Correlations with other pretreatment characteristics. Quantitative radiology of metastases is compared with other pretreatment characteristics in ability to predict survival in 102 patients with advanced Stage IV prostatic carcinoma. Using only two films, the posteroanterior chest and pelvis, radiologic quantitation of metastases is more precise than the other pretreatment characteristics in separating high-risk and low-risk patients. Quantitative radiology is a simple, noninvasive, effective method of stratifying patients in clinical trials or before aggressive therapy. The comparative ability of quantitative radiology and quantitative bone scans to separate high-risk and low-risk patients with advanced prostatic carcinoma can now be assessed."}
{"id": "PMID:473464", "title": "Impotence caused by pudendal arteriovenous fistula.", "content": "Partial impotence in a twenty-year-old was found, on angiography, to be the result of an arteriovenous fistula of the internal pudendal artery. This condition was corrected by corpus cavernosum revascularization.", "contents": "Impotence caused by pudendal arteriovenous fistula. Partial impotence in a twenty-year-old was found, on angiography, to be the result of an arteriovenous fistula of the internal pudendal artery. This condition was corrected by corpus cavernosum revascularization."}
{"id": "PMID:473465", "title": "Adenocarcinoma of gallbladder producing unilateral ureteral obstruction.", "content": "Ureteral obstruction is a common problem facing the urologist. Tumors, either primary in the urinary tract or metastases from other organs, may be the causal agent. Herein we present the first case reported of ureteral obstruction caused by metastases from adenocarcinoma of the gallbladder.", "contents": "Adenocarcinoma of gallbladder producing unilateral ureteral obstruction. Ureteral obstruction is a common problem facing the urologist. Tumors, either primary in the urinary tract or metastases from other organs, may be the causal agent. Herein we present the first case reported of ureteral obstruction caused by metastases from adenocarcinoma of the gallbladder."}
{"id": "PMID:473466", "title": "Hemangiopericytomas in male pelvis.", "content": "All cases of hemangiopericytomas involving the male pelvis which have been reported in the English literature with clinical data have been reviewed. Their clinical features, roentgenographic findings, pathologic features, and treatment are presented with an illustrative case. The tumor is an aggressive neoplasm which characteristically produces late local recurrences or metastases. Since the clinical behavior cannot be reliably predicted from its histologic appearance, all must be regarded as malignant. Primary treatment should be en bloc excision since a high recurrence rate results when simple enucleation or excision is performed. Long-term follow-up is mandatory, and local recurrences or metastases should be resected whenever feasible. Radiation and chemotherapy may convert a nonresectable lesion into a resectable one and provide significant palliation.", "contents": "Hemangiopericytomas in male pelvis. All cases of hemangiopericytomas involving the male pelvis which have been reported in the English literature with clinical data have been reviewed. Their clinical features, roentgenographic findings, pathologic features, and treatment are presented with an illustrative case. The tumor is an aggressive neoplasm which characteristically produces late local recurrences or metastases. Since the clinical behavior cannot be reliably predicted from its histologic appearance, all must be regarded as malignant. Primary treatment should be en bloc excision since a high recurrence rate results when simple enucleation or excision is performed. Long-term follow-up is mandatory, and local recurrences or metastases should be resected whenever feasible. Radiation and chemotherapy may convert a nonresectable lesion into a resectable one and provide significant palliation."}
{"id": "PMID:473467", "title": "Renal angiomyolipoma; polycystic kidney, and renal cell carcinoma in patient with tuberous sclerosis.", "content": "A patient with tuberous sclerosis presented with renal failure secondary to bilateral angiomyolipoma. The angiomyolipoma was associated with polycystic disease and a foci of renal cell carcinoma. This unusual combination has not been reported previously, although each entity has been described separately in tuberous sclerosis.", "contents": "Renal angiomyolipoma; polycystic kidney, and renal cell carcinoma in patient with tuberous sclerosis. A patient with tuberous sclerosis presented with renal failure secondary to bilateral angiomyolipoma. The angiomyolipoma was associated with polycystic disease and a foci of renal cell carcinoma. This unusual combination has not been reported previously, although each entity has been described separately in tuberous sclerosis."}
{"id": "PMID:473471", "title": "Oncocytoma of kidney.", "content": "Two cases of oncocytoma of the kidney are presented. The clinical and pathologic characteristics of this rare tumor are described and compared with those of renal cell carcinoma.", "contents": "Oncocytoma of kidney. Two cases of oncocytoma of the kidney are presented. The clinical and pathologic characteristics of this rare tumor are described and compared with those of renal cell carcinoma."}
{"id": "PMID:473472", "title": "Renal oncocytoma. Study of eight cases.", "content": "Eight renal oncocytomas are described. Clinical manifestations were present in 3 cases, with hematuria occurring in 2 and flank pain in 2. Tumors were between 2.5 and 10.0 cm. in diameter and showed considerable variation in histologic pattern. The tan coloration of some tumors may permit a naked-eye distinction from renal cell carcinoma. All tumors appeared to be benign.", "contents": "Renal oncocytoma. Study of eight cases. Eight renal oncocytomas are described. Clinical manifestations were present in 3 cases, with hematuria occurring in 2 and flank pain in 2. Tumors were between 2.5 and 10.0 cm. in diameter and showed considerable variation in histologic pattern. The tan coloration of some tumors may permit a naked-eye distinction from renal cell carcinoma. All tumors appeared to be benign."}
{"id": "PMID:473473", "title": "Epithelioid sarcoma of penis.", "content": "A rare case of an epithelioid sarcoma of the penis arising as a superficial plaque in the glans and causing pain on erection is reported. This tumor is characterized by a slow growth and infiltrative pattern with a high propensity for local recurrence. Proper treatment is wide local excision.", "contents": "Epithelioid sarcoma of penis. A rare case of an epithelioid sarcoma of the penis arising as a superficial plaque in the glans and causing pain on erection is reported. This tumor is characterized by a slow growth and infiltrative pattern with a high propensity for local recurrence. Proper treatment is wide local excision."}
{"id": "PMID:473475", "title": "Widespread dissemination of gelfoam particles complicating occlusion of renal circulation.", "content": "Embolization of renal circulation by Gelfoam particles in a thirty-two-year-old man with renal carcinoma was followed by retrograde regurgitation of the injected particles upstream, with resultant occlusion of the celiac trunk and the superior mesenteric artery. This was followed by a stormy clinical course, which the patient survived. Several possible explanations are offered for the occurrence of this complication.", "contents": "Widespread dissemination of gelfoam particles complicating occlusion of renal circulation. Embolization of renal circulation by Gelfoam particles in a thirty-two-year-old man with renal carcinoma was followed by retrograde regurgitation of the injected particles upstream, with resultant occlusion of the celiac trunk and the superior mesenteric artery. This was followed by a stormy clinical course, which the patient survived. Several possible explanations are offered for the occurrence of this complication."}
{"id": "PMID:473476", "title": "Pathologic vascularity of renal angiomyolipoma: cast corrosion and angiographic study.", "content": "Angiomyolipomas of the kidney at times have been preoperatively identified by the interlobar or interlobular artery eneurysms in the early vascular phase of renal arteriograms. A case report is presented in which a plastic cast corrosion technique was used postnephrectomy to demonstrate the presence of aneurysms identified angiographically prior to surgery. Portions of a cast corrosion model of a hypernephroma are also exhibited to compare and contrast pathologic vascular features of both types of tumors.", "contents": "Pathologic vascularity of renal angiomyolipoma: cast corrosion and angiographic study. Angiomyolipomas of the kidney at times have been preoperatively identified by the interlobar or interlobular artery eneurysms in the early vascular phase of renal arteriograms. A case report is presented in which a plastic cast corrosion technique was used postnephrectomy to demonstrate the presence of aneurysms identified angiographically prior to surgery. Portions of a cast corrosion model of a hypernephroma are also exhibited to compare and contrast pathologic vascular features of both types of tumors."}
{"id": "PMID:473477", "title": "Artifactual urethral stricture in male child.", "content": "Artifactual urethral obstruction at the suspensory ligament of the penis may be produced if the penis is left in its normally dependent position during a urethrogram. We present 2 such cases to re-emphasize this phenomenon so that caution is taken in interpreting distal bulbous urethral strictures.", "contents": "Artifactual urethral stricture in male child. Artifactual urethral obstruction at the suspensory ligament of the penis may be produced if the penis is left in its normally dependent position during a urethrogram. We present 2 such cases to re-emphasize this phenomenon so that caution is taken in interpreting distal bulbous urethral strictures."}
{"id": "PMID:473478", "title": "Compliance of the bladder: an attempt to establish normal values.", "content": "A study of 100 normal cystometries was undertaken to establish compliance values. The results seem to discourage the practical application of this parameter due to the absence of normal distribution and extreme scatter of values.", "contents": "Compliance of the bladder: an attempt to establish normal values. A study of 100 normal cystometries was undertaken to establish compliance values. The results seem to discourage the practical application of this parameter due to the absence of normal distribution and extreme scatter of values."}
{"id": "PMID:473482", "title": "Secondary bone tumours in the dog.", "content": "During a 2 1/2-year period 209 dogs with 218 malignant tumours were examined for skeletal metastases. There were 77 malignant tumours without visceral or skeletal metastases. Twenty-four (17%) of the 141 tumours with visceral metastases also had skeletal metastases. Ninety-eight of these tumours were of epithelial origin, and of these 21 (21.4%) had skeletal metastases. In 14 dogs, more than one bone had a metastatic tumour. The humerus, femur and vertebral column were most frequently involved. The lung was the most frequent site of metastatic tumours; then, in order, the liver, kidney and skeleton. The incidence of primary and secondary bone tumours was similar. Metastatic bone disease in the dog is far more common than is generally realized.", "contents": "Secondary bone tumours in the dog. During a 2 1/2-year period 209 dogs with 218 malignant tumours were examined for skeletal metastases. There were 77 malignant tumours without visceral or skeletal metastases. Twenty-four (17%) of the 141 tumours with visceral metastases also had skeletal metastases. Ninety-eight of these tumours were of epithelial origin, and of these 21 (21.4%) had skeletal metastases. In 14 dogs, more than one bone had a metastatic tumour. The humerus, femur and vertebral column were most frequently involved. The lung was the most frequent site of metastatic tumours; then, in order, the liver, kidney and skeleton. The incidence of primary and secondary bone tumours was similar. Metastatic bone disease in the dog is far more common than is generally realized."}
{"id": "PMID:473483", "title": "Intraocular melanoma in a horse.", "content": "Sudden unilateral blindness occurred in a 7-year-old grey gelding Quarterhorse. Ophthalmoscopy revealed a pigmented mass arising from the nasal ciliary body of the right eye and extending around the posterior surface of the lens, and there were pigmented particles in the vitreous. Examination of the enucleated globe showed a circumscribed, black, dense and symmetrically ovoid mass with sessile attachment to the nasal ciliary region and extension to posterior lens capsule, vitreous and along the vitreal face of the detached retina to the optic papilla. The mass was composed of heavily pigmented, plump, polyhedral cells that invaded the vitreous and the inner limiting membrane of peripapillary retina and optic papilla. It was considered to be a primary, malignant, intraocular melanoma arising from a large uveal nevus.", "contents": "Intraocular melanoma in a horse. Sudden unilateral blindness occurred in a 7-year-old grey gelding Quarterhorse. Ophthalmoscopy revealed a pigmented mass arising from the nasal ciliary body of the right eye and extending around the posterior surface of the lens, and there were pigmented particles in the vitreous. Examination of the enucleated globe showed a circumscribed, black, dense and symmetrically ovoid mass with sessile attachment to the nasal ciliary region and extension to posterior lens capsule, vitreous and along the vitreal face of the detached retina to the optic papilla. The mass was composed of heavily pigmented, plump, polyhedral cells that invaded the vitreous and the inner limiting membrane of peripapillary retina and optic papilla. It was considered to be a primary, malignant, intraocular melanoma arising from a large uveal nevus."}
{"id": "PMID:473484", "title": "Acinar cell carcinoma of the pancreas in a cat.", "content": "A feline pancreatic acinar cell carcinoma studied by light and electron microscopy was characterized by three growth patterns: acinar formation, colloid production and sheets of neoplastic cells. Ultrastructurally, the acinar origin of the tumor was determined by the presence of zymogen granules, some of which contained and released microfibrillar material.", "contents": "Acinar cell carcinoma of the pancreas in a cat. A feline pancreatic acinar cell carcinoma studied by light and electron microscopy was characterized by three growth patterns: acinar formation, colloid production and sheets of neoplastic cells. Ultrastructurally, the acinar origin of the tumor was determined by the presence of zymogen granules, some of which contained and released microfibrillar material."}
{"id": "PMID:473485", "title": "Spontaneous aortic atherosclerosis in sheep.", "content": "Aortas of 334 sheep 6 months to 6 years old were stained with Sudan IV. Sudanophilic lesions resembling early fatty streaks of man were detected in 50% of these aortas. Metastatic calcification was seen in eight (2.3%) aortas.", "contents": "Spontaneous aortic atherosclerosis in sheep. Aortas of 334 sheep 6 months to 6 years old were stained with Sudan IV. Sudanophilic lesions resembling early fatty streaks of man were detected in 50% of these aortas. Metastatic calcification was seen in eight (2.3%) aortas."}
{"id": "PMID:473486", "title": "The pregnant Syrian hamster as a model to study intravascular trophoblasts and associated maternal blood vessel changes.", "content": "In pregnant Syrian hamsters (Mesocricetus auratus) used as an animal model for studying the migration of fetal trophoblasts and the associated changes in maternal blood vessels, intravascular trophoblasts migrated well beyond the blood vessels of the uterus and into the vessels of the mesometrium. They migrated beyond the decidua of the uterus, into the lumina of maternal uterine and mesometrial arteries, but not into veins. The arterial changes, which were often segmental, resembled those seen in the decidua and consisted of a replacement of normal smooth muscle cells by poorly differentiated stromal cells. Ultrastructurally, the trophoblasts were either above or below maternal endothelial cells. They occurred also as single or multiple layers within the lumina of arteries that lacked an endothelial lining. Apparent penetration of the elastic membrane by the fetal trophoblasts brought them into close apposition to maternal cells in the arterial wall. Histochemical studies showed heightened metabolic activity of the intravascular trophoblasts as suggested by strong histochemical reactions to nonspecific esterase, succinic dehydrogenase and the glycerophosphate dehydrogenase reactions. Thus, these metabolically active fetal trophoblasts actively migrate into the maternal arterial system, resulting in loss of endothelial cells and changes in the wall of the maternal arteries similar to those in the decidua at the uteroplacental junction.", "contents": "The pregnant Syrian hamster as a model to study intravascular trophoblasts and associated maternal blood vessel changes. In pregnant Syrian hamsters (Mesocricetus auratus) used as an animal model for studying the migration of fetal trophoblasts and the associated changes in maternal blood vessels, intravascular trophoblasts migrated well beyond the blood vessels of the uterus and into the vessels of the mesometrium. They migrated beyond the decidua of the uterus, into the lumina of maternal uterine and mesometrial arteries, but not into veins. The arterial changes, which were often segmental, resembled those seen in the decidua and consisted of a replacement of normal smooth muscle cells by poorly differentiated stromal cells. Ultrastructurally, the trophoblasts were either above or below maternal endothelial cells. They occurred also as single or multiple layers within the lumina of arteries that lacked an endothelial lining. Apparent penetration of the elastic membrane by the fetal trophoblasts brought them into close apposition to maternal cells in the arterial wall. Histochemical studies showed heightened metabolic activity of the intravascular trophoblasts as suggested by strong histochemical reactions to nonspecific esterase, succinic dehydrogenase and the glycerophosphate dehydrogenase reactions. Thus, these metabolically active fetal trophoblasts actively migrate into the maternal arterial system, resulting in loss of endothelial cells and changes in the wall of the maternal arteries similar to those in the decidua at the uteroplacental junction."}
{"id": "PMID:473487", "title": "Studies on the pathogenesis of swine dysentery. II. Search for a cytotoxin in spirochetal broth cultures and colon content.", "content": "Broth cultures of Treponema hyodysenteriae and colonic content from pigs with swine dysentery were tested for cytotoxicity in cell cultures, erythrocyte suspensions and in ligated segments of pig colon. Live cells of T. hyodysenteriae attached to the surface of cells in all cultures tested but did not penetrate them nor cause morphologic change detectable by light microscopy. Only live T. hyodysenteriae caused erythrolysis. Broth cultures or colonic content sterilized by filtration or by disruption with ultrasound had no visible effect on the cell cultures, erythrocyte suspensions or the mucosa of ligated colonic segments.", "contents": "Studies on the pathogenesis of swine dysentery. II. Search for a cytotoxin in spirochetal broth cultures and colon content. Broth cultures of Treponema hyodysenteriae and colonic content from pigs with swine dysentery were tested for cytotoxicity in cell cultures, erythrocyte suspensions and in ligated segments of pig colon. Live cells of T. hyodysenteriae attached to the surface of cells in all cultures tested but did not penetrate them nor cause morphologic change detectable by light microscopy. Only live T. hyodysenteriae caused erythrolysis. Broth cultures or colonic content sterilized by filtration or by disruption with ultrasound had no visible effect on the cell cultures, erythrocyte suspensions or the mucosa of ligated colonic segments."}
{"id": "PMID:473488", "title": "Mesotheliomas and proliferative lesions of the testicular mesothelium produced in Fischer, Sprague-Dawley and Buffalo rats by methyl(acetoxymethyl)nitrosamine (DMN-OAc).", "content": "A single intraperitoneal dose of methyl(acetoxymethyl)nitrosamine (13 mg/kg body weight) given to 78 5-week-old male rats induced 25 mesotheliomas; two mesotheliomas were found in 67 control rats. All mesotheliomas arose from the peritesticular mesothelium and had a typical microscopic appearance of branching papillary fronds with a collagenous core covered by one or many layers of plump tumor cells. Cytoplasm of tumor cells contained material that reacted positively to a colloidal iron stain and was labile to hyaluronidase. In addition to frank mesotheliomas, 16 lesions, which we called atypical mesothelial proliferations, were found. These consisted of a single focus of plump mesothelial cells overlying an area of thick stroma. Often these foci included short, non-branched papillary projections above the surface of adjacent normal mesothelium. Twelve of the 16 lesions occurred in methyl(acetoxymethyl)nitrosamine-treated rats.", "contents": "Mesotheliomas and proliferative lesions of the testicular mesothelium produced in Fischer, Sprague-Dawley and Buffalo rats by methyl(acetoxymethyl)nitrosamine (DMN-OAc). A single intraperitoneal dose of methyl(acetoxymethyl)nitrosamine (13 mg/kg body weight) given to 78 5-week-old male rats induced 25 mesotheliomas; two mesotheliomas were found in 67 control rats. All mesotheliomas arose from the peritesticular mesothelium and had a typical microscopic appearance of branching papillary fronds with a collagenous core covered by one or many layers of plump tumor cells. Cytoplasm of tumor cells contained material that reacted positively to a colloidal iron stain and was labile to hyaluronidase. In addition to frank mesotheliomas, 16 lesions, which we called atypical mesothelial proliferations, were found. These consisted of a single focus of plump mesothelial cells overlying an area of thick stroma. Often these foci included short, non-branched papillary projections above the surface of adjacent normal mesothelium. Twelve of the 16 lesions occurred in methyl(acetoxymethyl)nitrosamine-treated rats."}
{"id": "PMID:473489", "title": "Mycotoxicosis produced in swine by cultural products of an isolate of Aspergillus ochraceus. I. Clinical observations and pathology.", "content": "Pigs fed a ration, 25% of which was rice culture, of Aspergillus ochraceus lost weight or failed to gain and became depressed. Some pigs died and most developed subcutaneous edema, hydrothorax, hydroperitoneum, pulmonary atelectasis, edema of the mesentery and perirenal edema. Microscopic lesions in addition to edema were primarily renal and consisted of tubular degeneration and necrosis, hyaline tubular casts, interstitial fibrosis and tubular cell regeneration. The first change found after 3 days was cytoplasmic vacuolation of the convoluted and straight segments of the proximal tubules. Necrotic proximal tubules were found after 4 days and after 9 days degeneration and necrosis involved predominantly proximal tubular segments. Pigs fed a ration, 12.5% of which was rice culture, for 8 weeks did not develop perirenal edema but had firm kidneys. Extensive interstitial fibrosis of the cortical labyrinth was the principal change. Within the fibrous connective tissue, some tubules were necrotic and others were atrophied.", "contents": "Mycotoxicosis produced in swine by cultural products of an isolate of Aspergillus ochraceus. I. Clinical observations and pathology. Pigs fed a ration, 25% of which was rice culture, of Aspergillus ochraceus lost weight or failed to gain and became depressed. Some pigs died and most developed subcutaneous edema, hydrothorax, hydroperitoneum, pulmonary atelectasis, edema of the mesentery and perirenal edema. Microscopic lesions in addition to edema were primarily renal and consisted of tubular degeneration and necrosis, hyaline tubular casts, interstitial fibrosis and tubular cell regeneration. The first change found after 3 days was cytoplasmic vacuolation of the convoluted and straight segments of the proximal tubules. Necrotic proximal tubules were found after 4 days and after 9 days degeneration and necrosis involved predominantly proximal tubular segments. Pigs fed a ration, 12.5% of which was rice culture, for 8 weeks did not develop perirenal edema but had firm kidneys. Extensive interstitial fibrosis of the cortical labyrinth was the principal change. Within the fibrous connective tissue, some tubules were necrotic and others were atrophied."}
{"id": "PMID:473490", "title": "Fish oil-induced yellow fat disease in rats. IV. Functional studies of the reticuloendothelial system.", "content": "In rats with \"stage S/E\" yellow fat disease an injection of colloidal carbon resulted in a marked reduction in the number of circulating platelets. The death rate of rats with experimental Listeria monocytogenes infection, the number of bacteria in their spleens and the decrease of bacteria in their spleens on the days after infection were the same in rats with yellow fat disease as in controls. The fact that the rats died during the first few days after infection also may indicate that their immunological resistance to L. monocytogenes was not altered by yellow fat disease.", "contents": "Fish oil-induced yellow fat disease in rats. IV. Functional studies of the reticuloendothelial system. In rats with \"stage S/E\" yellow fat disease an injection of colloidal carbon resulted in a marked reduction in the number of circulating platelets. The death rate of rats with experimental Listeria monocytogenes infection, the number of bacteria in their spleens and the decrease of bacteria in their spleens on the days after infection were the same in rats with yellow fat disease as in controls. The fact that the rats died during the first few days after infection also may indicate that their immunological resistance to L. monocytogenes was not altered by yellow fat disease."}
{"id": "PMID:473498", "title": "Serological study of ovine toxoplasmosis in Colombia: epidemiological study of a field outbreak.", "content": "A serological study, using the indirect haemagglutination test, of an outbreak of toxoplasmosis in a flock of blackface sheep is described. The serological aspects of the disease are discussed with reference to this incident, and possible sources of infection are suggested.", "contents": "Serological study of ovine toxoplasmosis in Colombia: epidemiological study of a field outbreak. A serological study, using the indirect haemagglutination test, of an outbreak of toxoplasmosis in a flock of blackface sheep is described. The serological aspects of the disease are discussed with reference to this incident, and possible sources of infection are suggested."}
{"id": "PMID:473499", "title": "The biological selenium status of livestock in Britain as indicated by sheep erythrocyte glutathione peroxidase activity.", "content": "The reliability of erythrocyte glutathione peroxidase activity as an indicator of selenium status in livestock is discussed. Based on this measurement, a survey is described of the biological selenium status of sheep on each of 329 farms in Britain. Results showed that 47 per cent of these farms were probably unable to provide grazing livestock with sufficient selenium to maintain blood levels greater than 0.075 microgram per ml. Increased selenium deficiency from the increasing use of home grown feeds as a major constituent of livestock rations may be causally related to the increase of white muscle disease and other selenium responsive diseases in Britain.", "contents": "The biological selenium status of livestock in Britain as indicated by sheep erythrocyte glutathione peroxidase activity. The reliability of erythrocyte glutathione peroxidase activity as an indicator of selenium status in livestock is discussed. Based on this measurement, a survey is described of the biological selenium status of sheep on each of 329 farms in Britain. Results showed that 47 per cent of these farms were probably unable to provide grazing livestock with sufficient selenium to maintain blood levels greater than 0.075 microgram per ml. Increased selenium deficiency from the increasing use of home grown feeds as a major constituent of livestock rations may be causally related to the increase of white muscle disease and other selenium responsive diseases in Britain."}
{"id": "PMID:473500", "title": "Reproductive performance of first parity sows.", "content": "An investigation of the reproductive performance of first parity sows is described. In a retrospective study it was found that the size of the second litter was significantly greater in those sows with a prolonged weaning to oestrus interval of more than 15 days. Deliberately delaying mating until at least 12 days after weaning produced a similar increase in second litter size. This improvement was not associated with an increase in ovulation rate.", "contents": "Reproductive performance of first parity sows. An investigation of the reproductive performance of first parity sows is described. In a retrospective study it was found that the size of the second litter was significantly greater in those sows with a prolonged weaning to oestrus interval of more than 15 days. Deliberately delaying mating until at least 12 days after weaning produced a similar increase in second litter size. This improvement was not associated with an increase in ovulation rate."}
{"id": "PMID:473504", "title": "Pregnancy diagnosis in sows: a comparison of the vaginal biopsy and Doppler ultrasound techniques.", "content": "Two pregnancy diagnosis techniques, vaginal biopsy and ultrasonic detection, were applied to 190 sows following service. Of 172 sows diagnosed pregnant by vaginal biopsy, five did not farrow. Of 18 sows judged not pregnant, one produced a litter. With the ultrasound technique, 175 sows were diagnosed pregnant, but 11 did not farrow. Of the remaining 15 sows considered not pregnant by the 39th day after service, four farrowed. The biopsy result was usually available up to 48 hours earlier than the ultrasound result. It was concluded that where laboratory facilities are available, the biopsy technique is the method of choice.", "contents": "Pregnancy diagnosis in sows: a comparison of the vaginal biopsy and Doppler ultrasound techniques. Two pregnancy diagnosis techniques, vaginal biopsy and ultrasonic detection, were applied to 190 sows following service. Of 172 sows diagnosed pregnant by vaginal biopsy, five did not farrow. Of 18 sows judged not pregnant, one produced a litter. With the ultrasound technique, 175 sows were diagnosed pregnant, but 11 did not farrow. Of the remaining 15 sows considered not pregnant by the 39th day after service, four farrowed. The biopsy result was usually available up to 48 hours earlier than the ultrasound result. It was concluded that where laboratory facilities are available, the biopsy technique is the method of choice."}
{"id": "PMID:473505", "title": "Pregnancy termination in the control of the tibial hemimelia syndrome in Galloway cattle.", "content": "A description is given of the symptoms, frequency and mode of inheritance of a lethal genetic defect in Galloway cattle known as the tibial hemimelia syndrome. Plans for the control of the defect are described, and the role of the Galloway test herd identified. Investigations in the test herd demonstrate that it is possible to detect the tibial hemimelia syndrome characteristics in 90-day-old fetuses and that those fetuses can be regularly and readily obtained undamaged by pregnancy termination using prostaglandin F2 alpha. The role of pregnancy termination in the control of the tibial hemimelia problem is discussed and ideas for its development presented.", "contents": "Pregnancy termination in the control of the tibial hemimelia syndrome in Galloway cattle. A description is given of the symptoms, frequency and mode of inheritance of a lethal genetic defect in Galloway cattle known as the tibial hemimelia syndrome. Plans for the control of the defect are described, and the role of the Galloway test herd identified. Investigations in the test herd demonstrate that it is possible to detect the tibial hemimelia syndrome characteristics in 90-day-old fetuses and that those fetuses can be regularly and readily obtained undamaged by pregnancy termination using prostaglandin F2 alpha. The role of pregnancy termination in the control of the tibial hemimelia problem is discussed and ideas for its development presented."}
{"id": "PMID:473506", "title": "A passive haemagglutination test for the detection of antibodies to the contagious equine metritis organism.", "content": "A passive haemagglutination test (PHT) which has been developed for the detection of antibodies to the contagious equine metritis organism (CEMO) in serum is described. Samples from each of 30 mares with metritis were positive with titres in the range 256 to 4096. Samples from each of 239 clinically normal mares and 30 colts and fillies believed not to have been exposed to CEMO were negative with titres of less than 256, the majority of samples (97 per cent) showing a titre of 32 or less.", "contents": "A passive haemagglutination test for the detection of antibodies to the contagious equine metritis organism. A passive haemagglutination test (PHT) which has been developed for the detection of antibodies to the contagious equine metritis organism (CEMO) in serum is described. Samples from each of 30 mares with metritis were positive with titres in the range 256 to 4096. Samples from each of 239 clinically normal mares and 30 colts and fillies believed not to have been exposed to CEMO were negative with titres of less than 256, the majority of samples (97 per cent) showing a titre of 32 or less."}
{"id": "PMID:473516", "title": "Fertility in buffaloes after oestrus synchronisation with cloprostenol and fixed time insemination.", "content": "Fifty-seven cycling buffalo cows of the river type were treated with two doses of 0.5 mg cloprostenol intramuscularly given 11 days apart. Each animal was inseminated twice at 72 and 96 hours after the second injection of cloprostenol. The first service conception rate diagnosed by rectal palpation at 90 days was 38.6 per cent. At the time of insemination the cervix was easily penetrable on both days in only 39 (68.4 per cent) of the animals. They were inseminated at or beyond the internal cervical os, while the others were inseminated in the cervical canal. There was a marked difference in conception rate between those receiving deep inseminations (48.7 per cent) and the others (16.7 per cent). In relation to the interval from calving to insemination the conception rates for those which had calved 60 to 90, 90 to 120 and 120 to 150 days earlier were 16.6, 36.4 and 55.5 per cent respectively. The use of cloprostenol treatment and fixed-time insemination is a useful method of overcoming the problem of oestrus detection in buffaloes. Acceptable levels of fertility can be obtained in those animals which have a sufficiently relaxed cervix to permit semen deposition at the internal os, provided the interval from calving to insemination is more than 90 days.", "contents": "Fertility in buffaloes after oestrus synchronisation with cloprostenol and fixed time insemination. Fifty-seven cycling buffalo cows of the river type were treated with two doses of 0.5 mg cloprostenol intramuscularly given 11 days apart. Each animal was inseminated twice at 72 and 96 hours after the second injection of cloprostenol. The first service conception rate diagnosed by rectal palpation at 90 days was 38.6 per cent. At the time of insemination the cervix was easily penetrable on both days in only 39 (68.4 per cent) of the animals. They were inseminated at or beyond the internal cervical os, while the others were inseminated in the cervical canal. There was a marked difference in conception rate between those receiving deep inseminations (48.7 per cent) and the others (16.7 per cent). In relation to the interval from calving to insemination the conception rates for those which had calved 60 to 90, 90 to 120 and 120 to 150 days earlier were 16.6, 36.4 and 55.5 per cent respectively. The use of cloprostenol treatment and fixed-time insemination is a useful method of overcoming the problem of oestrus detection in buffaloes. Acceptable levels of fertility can be obtained in those animals which have a sufficiently relaxed cervix to permit semen deposition at the internal os, provided the interval from calving to insemination is more than 90 days."}
{"id": "PMID:473517", "title": "Ovulation rate and egg recovery in cattle treated repeatedly with pregnant mare serum gonadotrophin and prostaglandin.", "content": "Fourteen heifers were superovulated five to 10 times using pregnant mare serum gonadotrophin (PMSG) and prostaglandin in a standardised regime which resulted in a mean interval of 42.1 days (SEM +/- 0.97) between treatments. There was a significant (P less than 0.02) fall in the mean ovulation rate at the second treatment when compared with the first but no significant difference between mean ovulation rate at the second and subsequent treatments. No significant difference between treatments in mean egg recovery rate or in the percentage of normal eggs recovered was demonstrated. Four heifers treated 10 times continued to respond with multiple ovulations on most occasions. At the end of the experiment 13 of the 14 heifers were inseminated and 12 conceived, requiring a mean of 1.67 services per pregnancy.", "contents": "Ovulation rate and egg recovery in cattle treated repeatedly with pregnant mare serum gonadotrophin and prostaglandin. Fourteen heifers were superovulated five to 10 times using pregnant mare serum gonadotrophin (PMSG) and prostaglandin in a standardised regime which resulted in a mean interval of 42.1 days (SEM +/- 0.97) between treatments. There was a significant (P less than 0.02) fall in the mean ovulation rate at the second treatment when compared with the first but no significant difference between mean ovulation rate at the second and subsequent treatments. No significant difference between treatments in mean egg recovery rate or in the percentage of normal eggs recovered was demonstrated. Four heifers treated 10 times continued to respond with multiple ovulations on most occasions. At the end of the experiment 13 of the 14 heifers were inseminated and 12 conceived, requiring a mean of 1.67 services per pregnancy."}
{"id": "PMID:473518", "title": "Increased incidence of still birth in piglets associated with levels of atmospheric carbon monoxide.", "content": "Raised levels of atmospheric carbon monoxide, associated with malfunctioning gas-fired creep heaters and inadequate ventilation, appeared responsible for an increased incidence of stillbirths in two herds. In one, a concentration of 180 to 200 ppm of carbon monoxide was readily produced. Both problems resolved when the ventilation and heating were improved, the incidence of stillbirths in one herd falling from 28 to 6.7 per cent. It is suggested that similar incidents may have occurred during cold weather but have been ascribed to other causes.", "contents": "Increased incidence of still birth in piglets associated with levels of atmospheric carbon monoxide. Raised levels of atmospheric carbon monoxide, associated with malfunctioning gas-fired creep heaters and inadequate ventilation, appeared responsible for an increased incidence of stillbirths in two herds. In one, a concentration of 180 to 200 ppm of carbon monoxide was readily produced. Both problems resolved when the ventilation and heating were improved, the incidence of stillbirths in one herd falling from 28 to 6.7 per cent. It is suggested that similar incidents may have occurred during cold weather but have been ascribed to other causes."}
{"id": "PMID:473519", "title": "Acorn poisoning in cattle.", "content": "Ten cows in a suckler herd totalling 60 sickened after grazing parkland which was heavily covered with acorns. Two died. The main clinical signs were dullness, anorexia and constipation. The main pathological findings were severe nephrosis and some intestinal ulceration. After removal to an acorn-free pasture, the remaining eight animals made a full recovery.", "contents": "Acorn poisoning in cattle. Ten cows in a suckler herd totalling 60 sickened after grazing parkland which was heavily covered with acorns. Two died. The main clinical signs were dullness, anorexia and constipation. The main pathological findings were severe nephrosis and some intestinal ulceration. After removal to an acorn-free pasture, the remaining eight animals made a full recovery."}
{"id": "PMID:473526", "title": "The definition of border disease: problems for the diagnostician.", "content": "Inocula derived from two sources of typical border disease (BD) and designated BP-77 and H-77 respectively, were prepared and injected into pregnant ewes of four breeds. The BP-77 inoculum produced higher serum neutralising antibody titres to the cytopathic BD virus than to the NADL strain of bovine virus diarrhoea virus. Only typical BD occurred among the progeny of all four breeds. In contrast H-77 produced higher titres to bovine virus diarrhoea than to BD and typical BD occurred only in the progeny of two of the four breeds. Progeny of the other two breeds appeared normal or had a range of malformations of the central nervous system but none of the characteristic clinical or pathological stigmata of BD. The existence of more than one strain of BD virus is confirmed. Virus strain/host genotype interactions may affect the character of disease in the progeny. The possible implications for diagnosis and control are discussed. It is concluded that the generally accepted criteria for diagnosis of BD are no longer adequate and that the disorder should be redesignated.", "contents": "The definition of border disease: problems for the diagnostician. Inocula derived from two sources of typical border disease (BD) and designated BP-77 and H-77 respectively, were prepared and injected into pregnant ewes of four breeds. The BP-77 inoculum produced higher serum neutralising antibody titres to the cytopathic BD virus than to the NADL strain of bovine virus diarrhoea virus. Only typical BD occurred among the progeny of all four breeds. In contrast H-77 produced higher titres to bovine virus diarrhoea than to BD and typical BD occurred only in the progeny of two of the four breeds. Progeny of the other two breeds appeared normal or had a range of malformations of the central nervous system but none of the characteristic clinical or pathological stigmata of BD. The existence of more than one strain of BD virus is confirmed. Virus strain/host genotype interactions may affect the character of disease in the progeny. The possible implications for diagnosis and control are discussed. It is concluded that the generally accepted criteria for diagnosis of BD are no longer adequate and that the disorder should be redesignated."}
{"id": "PMID:473527", "title": "Prevention of navel bleeding in piglets by preparturient administration of ascorbic acid.", "content": "Navel bleeding of newborn piglets was completely prevented by dosing the pregnant sows with ascorbic acid. The coagulation defect appeared to be caused by immature collagen which did not efficiently induce platelet plug formation. It seems that piglets depend upon their dams for their supply of vitamin C.", "contents": "Prevention of navel bleeding in piglets by preparturient administration of ascorbic acid. Navel bleeding of newborn piglets was completely prevented by dosing the pregnant sows with ascorbic acid. The coagulation defect appeared to be caused by immature collagen which did not efficiently induce platelet plug formation. It seems that piglets depend upon their dams for their supply of vitamin C."}
{"id": "PMID:473528", "title": "Observations on the selenium status of cattle in the north-east of Scotland.", "content": "Cattle on 18 of 21 farms in north-east Scotland were found to have low whole blood activities of the selenium-containing enzyme, glutathione peroxidase (less than 5 units per ml whole blood), and a low blood concentration of selenium (less than 0.05 mg per litre). These cattle had all been fed on locally produced feedstuffs without any mineral supplementation. The low selenium status in cattle occurred on farms with soils derived from a range of parent material, no one particular type predominating.", "contents": "Observations on the selenium status of cattle in the north-east of Scotland. Cattle on 18 of 21 farms in north-east Scotland were found to have low whole blood activities of the selenium-containing enzyme, glutathione peroxidase (less than 5 units per ml whole blood), and a low blood concentration of selenium (less than 0.05 mg per litre). These cattle had all been fed on locally produced feedstuffs without any mineral supplementation. The low selenium status in cattle occurred on farms with soils derived from a range of parent material, no one particular type predominating."}
{"id": "PMID:473529", "title": "Progesterone assays and rectal palpation in pre-service management of a dairy herd.", "content": "Fifty-one dairy cows were examined three times at weekly intervals during weeks 5 to 7 after calving. Rectal palpation of the ovaries and plasma progesterone assay were used to determine cyclic ovarian function. Eight of 51 cows exhibited true anoestrus by day 49 after calving. Only 23 of 43 cycling cows were served by day 77, the end of the target period for first service. In 77 per cent of 142 examinations, rectal palpation and progesterone assay gave compatible results. Most incompatibility occurred early or late inthe cycle, or in association with cystic structures in the ovaries. Eleven of the 51 cows had cysts in the ovaries but cyclicity and fertility were not adversely affected.", "contents": "Progesterone assays and rectal palpation in pre-service management of a dairy herd. Fifty-one dairy cows were examined three times at weekly intervals during weeks 5 to 7 after calving. Rectal palpation of the ovaries and plasma progesterone assay were used to determine cyclic ovarian function. Eight of 51 cows exhibited true anoestrus by day 49 after calving. Only 23 of 43 cycling cows were served by day 77, the end of the target period for first service. In 77 per cent of 142 examinations, rectal palpation and progesterone assay gave compatible results. Most incompatibility occurred early or late inthe cycle, or in association with cystic structures in the ovaries. Eleven of the 51 cows had cysts in the ovaries but cyclicity and fertility were not adversely affected."}
{"id": "PMID:473537", "title": "An evaluation of the API ZYM system as a means of classifying spirochaetes associated with swine dysentery.", "content": "Thirty-one strains of spirochaetes from pigs and two strains from poultry were tested in the API ZYM enzyme system. The spectrum of enzymatic reactions provided a means of differentiating between strains related to swine dysentery and strains not so related from pigs. The numerical coding system simplified the interpretation of the results.", "contents": "An evaluation of the API ZYM system as a means of classifying spirochaetes associated with swine dysentery. Thirty-one strains of spirochaetes from pigs and two strains from poultry were tested in the API ZYM enzyme system. The spectrum of enzymatic reactions provided a means of differentiating between strains related to swine dysentery and strains not so related from pigs. The numerical coding system simplified the interpretation of the results."}
{"id": "PMID:473538", "title": "Control of diarrhoea and death in home-bred dairy calves by bucket feeding pooled colostrum.", "content": "The feeding of colostrum to newborn calves from a bucket was found to be more effective in ensuring immune globulin uptake than leaving cow and calf together for two days post partum. A marked seasonal variation in the immune globulin status of calves, which could not readily be explained by management factors, was noted during two consecutive seasons.", "contents": "Control of diarrhoea and death in home-bred dairy calves by bucket feeding pooled colostrum. The feeding of colostrum to newborn calves from a bucket was found to be more effective in ensuring immune globulin uptake than leaving cow and calf together for two days post partum. A marked seasonal variation in the immune globulin status of calves, which could not readily be explained by management factors, was noted during two consecutive seasons."}
{"id": "PMID:473539", "title": "Intestinal leiomyoma in a cow.", "content": "A 10-year-old white Fulani Bunaji cow with clinical signs of ruminal stasis and melaena, was euthanised following discovery of a neoplasm in the spiral colon. Histopathological examination revealed a leiomyoma.", "contents": "Intestinal leiomyoma in a cow. A 10-year-old white Fulani Bunaji cow with clinical signs of ruminal stasis and melaena, was euthanised following discovery of a neoplasm in the spiral colon. Histopathological examination revealed a leiomyoma."}
{"id": "PMID:473540", "title": "Effects of fever and hyperthermia on the embryonic development of rabbits.", "content": "The effects of viral induced fever and environmental hyperthermia on embryonic development in pregnant rabbits are described. Embryonic development was not disturbed following fever, but there were anomalies in development following hyperthermia. Possible explanations for the different effects of hyperthermia and fever are discussed.", "contents": "Effects of fever and hyperthermia on the embryonic development of rabbits. The effects of viral induced fever and environmental hyperthermia on embryonic development in pregnant rabbits are described. Embryonic development was not disturbed following fever, but there were anomalies in development following hyperthermia. Possible explanations for the different effects of hyperthermia and fever are discussed."}
{"id": "PMID:473541", "title": "Effect of tipped horns on cattle bruising.", "content": "Six trials in Queensland compared the bruising of 960 cattle with untipped, tipped and no horns. In three of the trials the treatment groups were kept separate during transit to the abattoir. In the other trials all cattle were handled as the one group. Tipped and untipped cattle had similar bruising, whether sent for slaughter as separate groups or together. Thus tipping was ineffective in preventing bruising. Hornless cattle had significantly (P less than 0.05) less bruising than horned cattle when consigned as separate groups. However, this advantage was lost when hornless cattle were mixed with tipped or untipped animals.", "contents": "Effect of tipped horns on cattle bruising. Six trials in Queensland compared the bruising of 960 cattle with untipped, tipped and no horns. In three of the trials the treatment groups were kept separate during transit to the abattoir. In the other trials all cattle were handled as the one group. Tipped and untipped cattle had similar bruising, whether sent for slaughter as separate groups or together. Thus tipping was ineffective in preventing bruising. Hornless cattle had significantly (P less than 0.05) less bruising than horned cattle when consigned as separate groups. However, this advantage was lost when hornless cattle were mixed with tipped or untipped animals."}
{"id": "PMID:473542", "title": "Toxoplasmosis in a calf.", "content": "Gross post mortem and histopathological changes are described in a calf with toxoplasmosis. The major pathological changes were restricted to the lungs and kidneys and comprised interstitial pneumonia with multifocal necrosis, and glomerular necrosis. Many pseudocysts were present in both organs. The calf had been in close contact with young cats, one of which was killed and examined and shown to have a high antibody titre to Toxoplasma gondii, although there was little histological evidence of infection. No antibodies to toxoplasma were detected in serum from the dam of the calf, and accordingly, it is suggested that the cat was the source of infection for the calf.", "contents": "Toxoplasmosis in a calf. Gross post mortem and histopathological changes are described in a calf with toxoplasmosis. The major pathological changes were restricted to the lungs and kidneys and comprised interstitial pneumonia with multifocal necrosis, and glomerular necrosis. Many pseudocysts were present in both organs. The calf had been in close contact with young cats, one of which was killed and examined and shown to have a high antibody titre to Toxoplasma gondii, although there was little histological evidence of infection. No antibodies to toxoplasma were detected in serum from the dam of the calf, and accordingly, it is suggested that the cat was the source of infection for the calf."}
{"id": "PMID:473547", "title": "Spontaneous renal disease resembling hyperoxaluria in young lambs.", "content": "Five out of 60 purebred Suffolk lambs died when four to six weeks old with a nephrosis characterised by heavy deposits of crystals resembling calcium oxalate in the tubules. The source of oxalate was not clearly identified but may have resulted from oxalate-forming mould contamination of concentrates fed to both ewes and lambs.", "contents": "Spontaneous renal disease resembling hyperoxaluria in young lambs. Five out of 60 purebred Suffolk lambs died when four to six weeks old with a nephrosis characterised by heavy deposits of crystals resembling calcium oxalate in the tubules. The source of oxalate was not clearly identified but may have resulted from oxalate-forming mould contamination of concentrates fed to both ewes and lambs."}
{"id": "PMID:473548", "title": "Disturbance in the growth of the tibia and femur in dogs.", "content": "The clinical and radiographic features associated with premature cessation of the growth of the tibia (four) and femur (eight) in 12 dogs are described. Surgical correction of the resultant deformity was possible in only a few cases. Trauma was thought to be the cause of the local growth plate disorder.", "contents": "Disturbance in the growth of the tibia and femur in dogs. The clinical and radiographic features associated with premature cessation of the growth of the tibia (four) and femur (eight) in 12 dogs are described. Surgical correction of the resultant deformity was possible in only a few cases. Trauma was thought to be the cause of the local growth plate disorder."}
{"id": "PMID:473556", "title": "Border disease: a sequential study of surviving lambs and an assessment of its effect on profitability.", "content": "Pregnant Dorset horn gimmers inoculated with a non-cytopathic strain of the border disease virus produced \"hairy shaker\" lambs that were individually affected to a varying degree. Surviving lambs were observed for a period of up to 20 weeks when neurological signs were seen to gradually disappear. During this time live virus was isolated from a wide variety of sites in nearly all of the infected lambs. The infected animals grew at a significantly slower rate compared with the controls, particularly during the first 15 weeks. Several morphological defects are described the most consistent being the decreased weight of the central nervous system. By 20 weeks body-weights were about 20 per cent lower and carcase quality scores were reduced. An assessment is made of the economic losses attributable to BD taking into consideration carcase quality at 20 weeks and the perinatal losses.", "contents": "Border disease: a sequential study of surviving lambs and an assessment of its effect on profitability. Pregnant Dorset horn gimmers inoculated with a non-cytopathic strain of the border disease virus produced \"hairy shaker\" lambs that were individually affected to a varying degree. Surviving lambs were observed for a period of up to 20 weeks when neurological signs were seen to gradually disappear. During this time live virus was isolated from a wide variety of sites in nearly all of the infected lambs. The infected animals grew at a significantly slower rate compared with the controls, particularly during the first 15 weeks. Several morphological defects are described the most consistent being the decreased weight of the central nervous system. By 20 weeks body-weights were about 20 per cent lower and carcase quality scores were reduced. An assessment is made of the economic losses attributable to BD taking into consideration carcase quality at 20 weeks and the perinatal losses."}
{"id": "PMID:473557", "title": "Four cases of chondrodystrophy in fallow deer.", "content": "The gross and microscopic pathology in four cases of chondrodystrophy in a herd of captive fallow deer is described. The gross pathology was similar in all cases with shortening of all the bones, especially of the limbs, but at the microscopic level two distinct types of abnormality were present in the epiphyseal plates. In one type there was failure of proliferation of chondrocytes and in the other proliferation and vascularisation were irregular.", "contents": "Four cases of chondrodystrophy in fallow deer. The gross and microscopic pathology in four cases of chondrodystrophy in a herd of captive fallow deer is described. The gross pathology was similar in all cases with shortening of all the bones, especially of the limbs, but at the microscopic level two distinct types of abnormality were present in the epiphyseal plates. In one type there was failure of proliferation of chondrocytes and in the other proliferation and vascularisation were irregular."}
{"id": "PMID:473558", "title": "Bromocyclen poisoning in the dog.", "content": "An 18-week-old male German shepherd dog had a convulsion following the accidental ingestion of bromocyclen two hours previously. The dog then vomited and had a second convulsion. A pulse rate of 150 per minute and a respiratory rate of 54 per minute were recorded. The dog was treated with 2mg acepromazine and 0.6mg atropine administered intramuscularly (im) and repeated every four hours, 10ml of 20 per cent calcium borogluconate administered subcutaneously and 2ml penicillin and streptomycin im. Eighteen hours later, the respiratory rate was in excess of 60 per minute, and penicillin and streptomycin plus 2mg betamethasone were administered im. Only atropine was administered over the next 12 hours and then discontinued. Forty hours after the original convulsion, the respiratory rate had fallen to 30 per minute and the pulse rate to 84 per minute. A day later, the dog had fully recovered.", "contents": "Bromocyclen poisoning in the dog. An 18-week-old male German shepherd dog had a convulsion following the accidental ingestion of bromocyclen two hours previously. The dog then vomited and had a second convulsion. A pulse rate of 150 per minute and a respiratory rate of 54 per minute were recorded. The dog was treated with 2mg acepromazine and 0.6mg atropine administered intramuscularly (im) and repeated every four hours, 10ml of 20 per cent calcium borogluconate administered subcutaneously and 2ml penicillin and streptomycin im. Eighteen hours later, the respiratory rate was in excess of 60 per minute, and penicillin and streptomycin plus 2mg betamethasone were administered im. Only atropine was administered over the next 12 hours and then discontinued. Forty hours after the original convulsion, the respiratory rate had fallen to 30 per minute and the pulse rate to 84 per minute. A day later, the dog had fully recovered."}
{"id": "PMID:473564", "title": "Observations on avian pasteurellosis in Britain.", "content": "An investigation into the extent and significance of Pasteurella multocida infections of birds and poultry in Britain during a three year period (1974--1977) was undertaken. This was achieved by means of postal questionnaires, coupled with the biochemical and serological examination of bacterial isolates from 91 outbreaks in poultry and from nine cases in other avian species.", "contents": "Observations on avian pasteurellosis in Britain. An investigation into the extent and significance of Pasteurella multocida infections of birds and poultry in Britain during a three year period (1974--1977) was undertaken. This was achieved by means of postal questionnaires, coupled with the biochemical and serological examination of bacterial isolates from 91 outbreaks in poultry and from nine cases in other avian species."}
{"id": "PMID:473565", "title": "An outbreak of ringworm in a group of young cattle.", "content": "In an outbreak of ringworm, 30 out of 32 bull calves being reared intensively for beef showed infection. Weekly observations were made from their arrival at the farm at about one week old until slaughter at approximately 10 1/2 to 12 months of age. The outbreak lasted for 33 weeks and appeared to originate from two animals brought into the unit. Lesions occurred first, and most commonly, in the head region. The disease in individual animals lasted eight weeks and each lesion had a mean duration of 3.6 weeks. New lesions occurred after those elsewhere on the same animal had healed. The mean incubation period was estimated at 4.6 weeks. Spread of ringworm was probably mainly due to the licking and grooming activities of the calves' tongues.", "contents": "An outbreak of ringworm in a group of young cattle. In an outbreak of ringworm, 30 out of 32 bull calves being reared intensively for beef showed infection. Weekly observations were made from their arrival at the farm at about one week old until slaughter at approximately 10 1/2 to 12 months of age. The outbreak lasted for 33 weeks and appeared to originate from two animals brought into the unit. Lesions occurred first, and most commonly, in the head region. The disease in individual animals lasted eight weeks and each lesion had a mean duration of 3.6 weeks. New lesions occurred after those elsewhere on the same animal had healed. The mean incubation period was estimated at 4.6 weeks. Spread of ringworm was probably mainly due to the licking and grooming activities of the calves' tongues."}
{"id": "PMID:473566", "title": "Biochemical observations on beagle dog semen.", "content": "Semen samples were collected at weekly intervals for six weeks from eight sexually mature beagles previously shown to produce normal ejaculates. Seminal plasma and sperm fractions were separated by centrifugation and the sodium, potassium, alanine and aspartate aminotransferases, acid and alkaline phosphatase concentrations in the two fractions determined. Regression analysis of the mean weekly values obtained from physical and biochemical examination of the ejaculates showed that sodium ion concentration was highest in seminal plasma. The highest levels of aminotransferases were found in sperm fractions. Those enzymes may be indices of abnormal or damaged spermatozoa. Acid and alkaline phosphatase activity was 100 times greater in seminal plasma than in sperm fractions. Phosphatase concentrations are likely to be dependent on prostate activity. Measurement of acid phosphatase in canine semen therefore may be a useful index of prostate function. The motility of the semen samples was independent of the potassium concentration in seminal plasma. However, there was some evidence of a correlation between sperm motility and the enzyme and sodium content of seminal plasma.", "contents": "Biochemical observations on beagle dog semen. Semen samples were collected at weekly intervals for six weeks from eight sexually mature beagles previously shown to produce normal ejaculates. Seminal plasma and sperm fractions were separated by centrifugation and the sodium, potassium, alanine and aspartate aminotransferases, acid and alkaline phosphatase concentrations in the two fractions determined. Regression analysis of the mean weekly values obtained from physical and biochemical examination of the ejaculates showed that sodium ion concentration was highest in seminal plasma. The highest levels of aminotransferases were found in sperm fractions. Those enzymes may be indices of abnormal or damaged spermatozoa. Acid and alkaline phosphatase activity was 100 times greater in seminal plasma than in sperm fractions. Phosphatase concentrations are likely to be dependent on prostate activity. Measurement of acid phosphatase in canine semen therefore may be a useful index of prostate function. The motility of the semen samples was independent of the potassium concentration in seminal plasma. However, there was some evidence of a correlation between sperm motility and the enzyme and sodium content of seminal plasma."}
{"id": "PMID:473573", "title": "Ischaemic neuromyopathy in cats.", "content": "The effect of ischaemic neuromyopathy in cats on peripheral muscles and nerves is described. Motor function was severely decreased distal to the stifle particularly in the cranial tibial muscles. Skin sensation was absent distal to the mid tibial or hock level. The affected muscles were often hard and painful. Improvement of motor function began two to three weeks after onset and complete recovery could occur. Conduction to the interosseous and anterior tibial muscles is absent or severely reduced initially but returned and improved within two weeks. A few peripheral nerve fibres could survive the ischaemia, others showed varying defects on the myelin sheath but the majority degenerated. Shorter term recoveries were probably due to repair of the myelin sheath. Regenerated nerve fibres were also demonstrated. The cranial tibial muscles were commonly infarcted while less severe myopathic changes were found in the gastrocnemii. Provided further ischaemic episodes can be prevented the prognosis in these cases appears good.", "contents": "Ischaemic neuromyopathy in cats. The effect of ischaemic neuromyopathy in cats on peripheral muscles and nerves is described. Motor function was severely decreased distal to the stifle particularly in the cranial tibial muscles. Skin sensation was absent distal to the mid tibial or hock level. The affected muscles were often hard and painful. Improvement of motor function began two to three weeks after onset and complete recovery could occur. Conduction to the interosseous and anterior tibial muscles is absent or severely reduced initially but returned and improved within two weeks. A few peripheral nerve fibres could survive the ischaemia, others showed varying defects on the myelin sheath but the majority degenerated. Shorter term recoveries were probably due to repair of the myelin sheath. Regenerated nerve fibres were also demonstrated. The cranial tibial muscles were commonly infarcted while less severe myopathic changes were found in the gastrocnemii. Provided further ischaemic episodes can be prevented the prognosis in these cases appears good."}
{"id": "PMID:473574", "title": "Toxocara and ascaris infection in British pigs: a serological survey.", "content": "A serological survey of growing pigs from farms throughout Great Britain revealed that antibodies to toxocara and ascaris larval antigens were present in 4.5 per cent and 39.3 per cent respectively. Heavy infection with toxocara occurred only rarely and it is considered that this parasite is unlikely to be an important cause of chronic focal interstitial hepatitis. Antibodies to both nematodes were detected more frequently in heavy hogs than in pigs of lighter weight. Reginal differences in the prevalence of antibodies were also observed.", "contents": "Toxocara and ascaris infection in British pigs: a serological survey. A serological survey of growing pigs from farms throughout Great Britain revealed that antibodies to toxocara and ascaris larval antigens were present in 4.5 per cent and 39.3 per cent respectively. Heavy infection with toxocara occurred only rarely and it is considered that this parasite is unlikely to be an important cause of chronic focal interstitial hepatitis. Antibodies to both nematodes were detected more frequently in heavy hogs than in pigs of lighter weight. Reginal differences in the prevalence of antibodies were also observed."}
{"id": "PMID:473582", "title": "Observations on the technical quality of radiographs submitted to a veterinary college.", "content": "The technical quality of 75 sets of radiographs submitted from general practice was assessed. The most serious faults were underdevelopment and failure to collimate the beam adequately. The correction of these two faults should significantly improve the overall standard of veterinary practice radiography.", "contents": "Observations on the technical quality of radiographs submitted to a veterinary college. The technical quality of 75 sets of radiographs submitted from general practice was assessed. The most serious faults were underdevelopment and failure to collimate the beam adequately. The correction of these two faults should significantly improve the overall standard of veterinary practice radiography."}
{"id": "PMID:473674", "title": "[Effect of flavonoids on aspects of nitrogen metabolism in experimental uremia].", "content": "Content of free ammonia and glutamine amide nitrogen was increased but content of protein amide nitrogen was decreased in brain tissue after bilateral nephrectomy of rats causing development an experimental uremia; an increased synthesis of urea and activation of arginase were observed in liver tissue. Flavonoids robinine and hyperine decreased the content of free ammonia, increased the content of glutamine amide nitrogen as well as of protein amide nitrogen in rat brain; robinine decreased slightly the urea synthesis and the arginase activity in liver slices of the nephrectomized rats.", "contents": "[Effect of flavonoids on aspects of nitrogen metabolism in experimental uremia]. Content of free ammonia and glutamine amide nitrogen was increased but content of protein amide nitrogen was decreased in brain tissue after bilateral nephrectomy of rats causing development an experimental uremia; an increased synthesis of urea and activation of arginase were observed in liver tissue. Flavonoids robinine and hyperine decreased the content of free ammonia, increased the content of glutamine amide nitrogen as well as of protein amide nitrogen in rat brain; robinine decreased slightly the urea synthesis and the arginase activity in liver slices of the nephrectomized rats."}
{"id": "PMID:473676", "title": "[Tryptamine metabolic study in man].", "content": "A modified norharmane method was used for estimation of tryptamine in human urine at normal state. Tryptamine excretion did not depend on diuresis. Administration of neomycin did not affect the tryptamine excretion, which is inconsistent with the hypothesis on bacterial origin of the amine. Dynamics of tryptamine excretion was studied after administration of tryptophane, inhibitors of monoamine oxidase and their combinations. Sydnophen and sydnocarb caused hypotryptaminuria. Marked hypertryptaminuria was observed in severe chronic hepatitis and liver cirrhosis.", "contents": "[Tryptamine metabolic study in man]. A modified norharmane method was used for estimation of tryptamine in human urine at normal state. Tryptamine excretion did not depend on diuresis. Administration of neomycin did not affect the tryptamine excretion, which is inconsistent with the hypothesis on bacterial origin of the amine. Dynamics of tryptamine excretion was studied after administration of tryptophane, inhibitors of monoamine oxidase and their combinations. Sydnophen and sydnocarb caused hypotryptaminuria. Marked hypertryptaminuria was observed in severe chronic hepatitis and liver cirrhosis."}
{"id": "PMID:473677", "title": "[Effect of typhoid endotoxin and lipid A on the phospholipid content in liver tissue].", "content": "Content of phospholipid fractions was altered in mice liver tissue after intraperitoneal administration of typhoid lipopolysaccharide and of lipid A, isolated from the lipopolysaccharide. The intoxication was accompanied by an increase in content of choline-containing phospholipids within 6 and 24 hrs as well as by an alteration in the ratio of the fractions. Effect of lipid A on the tissue phospholipid spectra resembled the effect of the lipopolysaccharide, but was less prolonged. The lipopolysaccharide and lipid A altered the stability of phosphatidyl choline and cardiolipin bonds with the components of liver cells.", "contents": "[Effect of typhoid endotoxin and lipid A on the phospholipid content in liver tissue]. Content of phospholipid fractions was altered in mice liver tissue after intraperitoneal administration of typhoid lipopolysaccharide and of lipid A, isolated from the lipopolysaccharide. The intoxication was accompanied by an increase in content of choline-containing phospholipids within 6 and 24 hrs as well as by an alteration in the ratio of the fractions. Effect of lipid A on the tissue phospholipid spectra resembled the effect of the lipopolysaccharide, but was less prolonged. The lipopolysaccharide and lipid A altered the stability of phosphatidyl choline and cardiolipin bonds with the components of liver cells."}
{"id": "PMID:473675", "title": "[Changes in the isoenzymatic makeup of pyruvate kinase in hepatic carcinogenesis].", "content": "Together with an increase in the total activity of pyruvate kinase, alterations in the isoenzyme spectrum were observed in hepatocarcinogenesis caused by treatment of rats with diethylinitrosamine. The alterations might be divided into two steps: initial, reversible changes and changes immediately related to development of the blastomatous process. The total activity of pyruvate kinase was gradually increased with appearance of hyperplastic symptoms as well as of primary tumoral foci in liver tissue. The M2-type of pyruvate kinase tended to exhibit activation. In primary hepatomas the L-type of the enzyme was decreased and activity of the M2-type was increased.", "contents": "[Changes in the isoenzymatic makeup of pyruvate kinase in hepatic carcinogenesis]. Together with an increase in the total activity of pyruvate kinase, alterations in the isoenzyme spectrum were observed in hepatocarcinogenesis caused by treatment of rats with diethylinitrosamine. The alterations might be divided into two steps: initial, reversible changes and changes immediately related to development of the blastomatous process. The total activity of pyruvate kinase was gradually increased with appearance of hyperplastic symptoms as well as of primary tumoral foci in liver tissue. The M2-type of pyruvate kinase tended to exhibit activation. In primary hepatomas the L-type of the enzyme was decreased and activity of the M2-type was increased."}
{"id": "PMID:473678", "title": "[Enzymatic characteristics of a peroxisome fraction isolated by the use of hypotonic treatment of subcellular structures].", "content": "A peroxisome fraction was isolated from rat liver tissue using hypotonic treatment of mitochondria and lysosomes. In the fraction obtained activity of marker enzymes was studied in various subcellular structures. Content of mitochondria, lysosomes and microsomes in the peroxisome fraction was about 15-20% of the total protein in the fraction. The results of hypotonic treatment of lysosomes and mitochondria are considered. Flotation activity of lysosomes was decreased to 1.16-1.20 g/cm3 after osmotic lysis and elimination of matrix enzymes from these organelles.", "contents": "[Enzymatic characteristics of a peroxisome fraction isolated by the use of hypotonic treatment of subcellular structures]. A peroxisome fraction was isolated from rat liver tissue using hypotonic treatment of mitochondria and lysosomes. In the fraction obtained activity of marker enzymes was studied in various subcellular structures. Content of mitochondria, lysosomes and microsomes in the peroxisome fraction was about 15-20% of the total protein in the fraction. The results of hypotonic treatment of lysosomes and mitochondria are considered. Flotation activity of lysosomes was decreased to 1.16-1.20 g/cm3 after osmotic lysis and elimination of matrix enzymes from these organelles."}
{"id": "PMID:473680", "title": "[Mitochondrial thymidine kinase isoenzymes from normal and proliferating rat liver tissues].", "content": "Isoenzyme composition of thymidine kinase was studied in submitochondrial fractions of liver tissue with various proliferative activity (intact, regenerating livers and Zhaidel ascites hepatoma) using polyacrylamide gel disc electrophoresis. Three zones, corresponding to proteins with Rf 0.1-0.2 (I), Rf 0.5-0.55 (II) and Rf 0.85-0.87 (III) and exhibiting thymidine kinase activity, were found in fractions of cytoplasmic and mitochondrial matrix proteins from resting and proliferating rat liver tissues. In fractions of outer and inner mitochondrial membranes three zones of the enzymatic activity were also observed but two of them did not coincide in the Rf value with the thymidine kinase isoenzymes from cytoplasmic fraction and mitochondrial matrix: I Rf 0.1-0.16, II Rf 0.35-0.4 and III Rf 0.62-0.68. Redistribution of the enzymatic activity between thymidine kinase isozymes occurred in conversion of liver tissue from the resting state to increased proliferation. In these cases slowly migrating enzymatic fraction (Rf 0.1-0.2) was activated in mitochondrial matrix and membranes; formation of TMP, catalyzed by isozymes with fast mobility (Rf 0.5-0.55 in matrix and Rf 0.62-0.68 in membrane fractions of mitochondria), which are typical for intact liver tissue, was decreased, respectively.", "contents": "[Mitochondrial thymidine kinase isoenzymes from normal and proliferating rat liver tissues]. Isoenzyme composition of thymidine kinase was studied in submitochondrial fractions of liver tissue with various proliferative activity (intact, regenerating livers and Zhaidel ascites hepatoma) using polyacrylamide gel disc electrophoresis. Three zones, corresponding to proteins with Rf 0.1-0.2 (I), Rf 0.5-0.55 (II) and Rf 0.85-0.87 (III) and exhibiting thymidine kinase activity, were found in fractions of cytoplasmic and mitochondrial matrix proteins from resting and proliferating rat liver tissues. In fractions of outer and inner mitochondrial membranes three zones of the enzymatic activity were also observed but two of them did not coincide in the Rf value with the thymidine kinase isoenzymes from cytoplasmic fraction and mitochondrial matrix: I Rf 0.1-0.16, II Rf 0.35-0.4 and III Rf 0.62-0.68. Redistribution of the enzymatic activity between thymidine kinase isozymes occurred in conversion of liver tissue from the resting state to increased proliferation. In these cases slowly migrating enzymatic fraction (Rf 0.1-0.2) was activated in mitochondrial matrix and membranes; formation of TMP, catalyzed by isozymes with fast mobility (Rf 0.5-0.55 in matrix and Rf 0.62-0.68 in membrane fractions of mitochondria), which are typical for intact liver tissue, was decreased, respectively."}
{"id": "PMID:473679", "title": "[Postmortem inhibition of RNA synthesis in the liver and skeletal muscles of rats].", "content": "RNA synthesis was studied at various postmortal periods in liver and sceletal muscle slices, incubated in Krebs-Ringer solution (pH 7.4) containing NaCl, KCl, CaCl2, KH2PO4, MgSO4, NaHCO3, glucose, normal horse blood serum, penicillin, heparin, mixture of 20 amino acids and 14C-uridine. The higher rate of postmortal inactivation of RNA synthesis was found in liver tissue as compared to muscles; decreased stability of total endogenous RNA from liver tissue was observed under conditions of autolysis. Various dynamics occurred in dissociation of endogenous RNA from sceletal muscles studied at early (6 hrs) and late (24 hrs) postmortal periods and maintained in Krebs-Ringer solution. As shown by analysis of newly formed RNA from liver tissue using disc electrophoresis, synthesis of high molecular RNA (28 S, 18 S) was primarily impaired in cases of postmortal inhibition of the synthesis; liver cells maintained their capacity for the synthesis of low molecular RNA within 3 hrs after death of the animals.", "contents": "[Postmortem inhibition of RNA synthesis in the liver and skeletal muscles of rats]. RNA synthesis was studied at various postmortal periods in liver and sceletal muscle slices, incubated in Krebs-Ringer solution (pH 7.4) containing NaCl, KCl, CaCl2, KH2PO4, MgSO4, NaHCO3, glucose, normal horse blood serum, penicillin, heparin, mixture of 20 amino acids and 14C-uridine. The higher rate of postmortal inactivation of RNA synthesis was found in liver tissue as compared to muscles; decreased stability of total endogenous RNA from liver tissue was observed under conditions of autolysis. Various dynamics occurred in dissociation of endogenous RNA from sceletal muscles studied at early (6 hrs) and late (24 hrs) postmortal periods and maintained in Krebs-Ringer solution. As shown by analysis of newly formed RNA from liver tissue using disc electrophoresis, synthesis of high molecular RNA (28 S, 18 S) was primarily impaired in cases of postmortal inhibition of the synthesis; liver cells maintained their capacity for the synthesis of low molecular RNA within 3 hrs after death of the animals."}
{"id": "PMID:473681", "title": "[Induction of experimental allergic encephalomyelitis with encephalitogenic myelin proteins from the spinal cord of a bull].", "content": "A homogenous base protein, exhibiting high encephalitogenic activity towards guinea pigs and rabbits, was isolated from bovine spinal cord using column chromatography. Administration of the preparation obtained together with Freund's adjuvant into the animals caused development of neurogic symptoms typical for experimental allergic encephalomyelitis as well as formation of demyelinization foci both in spinal cord and in brain of guinea pigs and rabbits.", "contents": "[Induction of experimental allergic encephalomyelitis with encephalitogenic myelin proteins from the spinal cord of a bull]. A homogenous base protein, exhibiting high encephalitogenic activity towards guinea pigs and rabbits, was isolated from bovine spinal cord using column chromatography. Administration of the preparation obtained together with Freund's adjuvant into the animals caused development of neurogic symptoms typical for experimental allergic encephalomyelitis as well as formation of demyelinization foci both in spinal cord and in brain of guinea pigs and rabbits."}
{"id": "PMID:473683", "title": "[Change in the enzymatic activity of liver carbohydrate metabolism after the one-time carbon tertrachloride poisoning of rats].", "content": "Activity of gluconeogenesic enzymes was more distinctly decreased than the activity of glycolytic enzymes within 8 and 24 hrs after a single intoxication of rats with CCl4. Within 72 hrs the enzymatic activity of glycolysis and gluconeogenesis was similar but the activity of the enzymes participating in the hexose monophosphate shunt was 2-3-fold higher as compared with the patterns characteristic for the intact animals. Mechanisms of these alterations are discussed; they are apparently related to the change in hepatocyte population due to necrosis and regeneration as well as to localization of enzymes of gluconeogenesis in central part of liver lobule.", "contents": "[Change in the enzymatic activity of liver carbohydrate metabolism after the one-time carbon tertrachloride poisoning of rats]. Activity of gluconeogenesic enzymes was more distinctly decreased than the activity of glycolytic enzymes within 8 and 24 hrs after a single intoxication of rats with CCl4. Within 72 hrs the enzymatic activity of glycolysis and gluconeogenesis was similar but the activity of the enzymes participating in the hexose monophosphate shunt was 2-3-fold higher as compared with the patterns characteristic for the intact animals. Mechanisms of these alterations are discussed; they are apparently related to the change in hepatocyte population due to necrosis and regeneration as well as to localization of enzymes of gluconeogenesis in central part of liver lobule."}
{"id": "PMID:473685", "title": "[Isolation of renin from human kidneys].", "content": "Isolation and partial purification of renin from human kidney was carried out as follows: defatting of the homogenized kidney; extraction with methyl cellosolve; chromatography on DEAE cellulose; affinity chromatography using immobilized pepstain. Activity of the preparation obtained was 0.1 GU/mg of protein. The preparation of human renin might be used for measurement of renin activity in blood of patients.", "contents": "[Isolation of renin from human kidneys]. Isolation and partial purification of renin from human kidney was carried out as follows: defatting of the homogenized kidney; extraction with methyl cellosolve; chromatography on DEAE cellulose; affinity chromatography using immobilized pepstain. Activity of the preparation obtained was 0.1 GU/mg of protein. The preparation of human renin might be used for measurement of renin activity in blood of patients."}
{"id": "PMID:473686", "title": "[Effect of the total flavonoids from red clover and chick-pea on the lipid content in the blood and liver of rats].", "content": "Total flavonoids from clover red and nut, containing isoflavones, decreased the content of cholesterol and triglycerides in intact rats, inhibited the development of hyperlipidemia, caused by Triton WR-1339, prevented accumulation of triglycerides in rat liver and blood caused by ethanol administration. The flavonoids from these plants inhibited lipolysis, stimulated by adrenaline.", "contents": "[Effect of the total flavonoids from red clover and chick-pea on the lipid content in the blood and liver of rats]. Total flavonoids from clover red and nut, containing isoflavones, decreased the content of cholesterol and triglycerides in intact rats, inhibited the development of hyperlipidemia, caused by Triton WR-1339, prevented accumulation of triglycerides in rat liver and blood caused by ethanol administration. The flavonoids from these plants inhibited lipolysis, stimulated by adrenaline."}
{"id": "PMID:473687", "title": "[Glutathione: dehydroascorbate-oxidoreductase activity in rat tissues].", "content": "Glutathione: dehydroascorbate reductase activity was studied in rat liver, heart, spleen, lungs as well as in Zajdela hepatoma. Correlation between the activities of glutathione reductase and glutathione: dehydroascorbate reductase was observed in all the tissues studied. Glutathione: dehydroascorbate reductase activity was higher in Zajdela hepatoma as compared with the rat liver. The role of glutathione: dehydroascorbate reductase in regulation of antioxidative activity and of cell division is discussed.", "contents": "[Glutathione: dehydroascorbate-oxidoreductase activity in rat tissues]. Glutathione: dehydroascorbate reductase activity was studied in rat liver, heart, spleen, lungs as well as in Zajdela hepatoma. Correlation between the activities of glutathione reductase and glutathione: dehydroascorbate reductase was observed in all the tissues studied. Glutathione: dehydroascorbate reductase activity was higher in Zajdela hepatoma as compared with the rat liver. The role of glutathione: dehydroascorbate reductase in regulation of antioxidative activity and of cell division is discussed."}
{"id": "PMID:473682", "title": "[Hexokinase and lactate dehydrogenase activity and isoenzymatic makeup of the soluble fraction of dissimilarly functioning rabbit muscles normally and in experimental allergic encephalomyelitis].", "content": "Only one isozyme of hexokinase (type I) was found in a soluble fraction of smooth muscle of rabbit stomach using column chromatography and polyacrylamide gel electrophoresis. By the pattern of metabolism the smooth muscle of rabbit stomach accupies an intermediate position between rapidly and slowly contracting sceletal muscles, approaching to musculus soleus by the activity and isozyme spectra of hexokinase and lactate dehydrogenase (LDH). Activity of these enzymes was altered not uniformly in dissimilarly functioning muscles of rabbits with experimental allergic encephalomyelitis: it was increased in musculus gastrocnemius of rabbits and decreased in soleus or in smooth muscles. LDH isoenzyme spectra changed towards an increase in aerobic H-subunits and decrease in anaerobic M-subunits in the soluble fraction of musculus gastrocnemius of rabbits with experimental allergic encephalomyelitis. Content of LDH-5 and LDH-4 was about 2-fold increased and content of LDH-1 and LDH-2 was decreased in musculus soleus.", "contents": "[Hexokinase and lactate dehydrogenase activity and isoenzymatic makeup of the soluble fraction of dissimilarly functioning rabbit muscles normally and in experimental allergic encephalomyelitis]. Only one isozyme of hexokinase (type I) was found in a soluble fraction of smooth muscle of rabbit stomach using column chromatography and polyacrylamide gel electrophoresis. By the pattern of metabolism the smooth muscle of rabbit stomach accupies an intermediate position between rapidly and slowly contracting sceletal muscles, approaching to musculus soleus by the activity and isozyme spectra of hexokinase and lactate dehydrogenase (LDH). Activity of these enzymes was altered not uniformly in dissimilarly functioning muscles of rabbits with experimental allergic encephalomyelitis: it was increased in musculus gastrocnemius of rabbits and decreased in soleus or in smooth muscles. LDH isoenzyme spectra changed towards an increase in aerobic H-subunits and decrease in anaerobic M-subunits in the soluble fraction of musculus gastrocnemius of rabbits with experimental allergic encephalomyelitis. Content of LDH-5 and LDH-4 was about 2-fold increased and content of LDH-1 and LDH-2 was decreased in musculus soleus."}
{"id": "PMID:473688", "title": "[Effect of the early postnatal induction of microsomal enzymes on their activity and the cholesterol content in the blood of adult mice from a hypercholesteremic line].", "content": "Activity of microsomal enzymes and the patterns of cholesterol metabolism were studied in mice of WSR/y strain, characterized by spontaneous development of atherosclerosis within the later periods of life, after early postnatal administration of an inductor of the enzymes 3-acetate-16 alpha-isothiocyanopregnenolone (ATCP). Administration of ATCP into newborn mice of SWR/y strain, from the 2nd up to 16th day after birth, led to a stable increase in activity of arylhydrocarbonate hydroxylase (an enzyme participating in unspecific metabolism of drugs), which was observed during the whole experimental period (4 months). The treatment with ATCP caused also a distinct increase in activity of cholesterol-7 alpha-hydroxylase (a key enzyme of cholesterol biotransformation and elimination) as well as a considerable decrease in content of cholesterol and lipoprotein atherogenic fractions in blood serum. The rate of cholesterol biosynthesis was similar both in mice treated with ATCP and in the control animals.", "contents": "[Effect of the early postnatal induction of microsomal enzymes on their activity and the cholesterol content in the blood of adult mice from a hypercholesteremic line]. Activity of microsomal enzymes and the patterns of cholesterol metabolism were studied in mice of WSR/y strain, characterized by spontaneous development of atherosclerosis within the later periods of life, after early postnatal administration of an inductor of the enzymes 3-acetate-16 alpha-isothiocyanopregnenolone (ATCP). Administration of ATCP into newborn mice of SWR/y strain, from the 2nd up to 16th day after birth, led to a stable increase in activity of arylhydrocarbonate hydroxylase (an enzyme participating in unspecific metabolism of drugs), which was observed during the whole experimental period (4 months). The treatment with ATCP caused also a distinct increase in activity of cholesterol-7 alpha-hydroxylase (a key enzyme of cholesterol biotransformation and elimination) as well as a considerable decrease in content of cholesterol and lipoprotein atherogenic fractions in blood serum. The rate of cholesterol biosynthesis was similar both in mice treated with ATCP and in the control animals."}
{"id": "PMID:473689", "title": "[Effect of light deprivation on GABA metabolism in subcellular fractions of the rabbit visual system].", "content": "Specific activity of glutamate decarboxylase was decreased in subfractions of light and heavy synaptosomes, and gamma-aminobutyric acid (GABA) transaminase activity--also in subfractions of free mitochondria, isolated from visual zone of brain cortex and of anterior mesencephalon but not from locomotive region of brain cortex of the light-deprived rabbits. Decrease in the ratio GABA transaminase/glutamate decarboxylase indicated distinct inhibition of GABA degradation as compared with its synthesis due to absence of sensory impulsation within the early periods of postnatal ontogenesis of the animals.", "contents": "[Effect of light deprivation on GABA metabolism in subcellular fractions of the rabbit visual system]. Specific activity of glutamate decarboxylase was decreased in subfractions of light and heavy synaptosomes, and gamma-aminobutyric acid (GABA) transaminase activity--also in subfractions of free mitochondria, isolated from visual zone of brain cortex and of anterior mesencephalon but not from locomotive region of brain cortex of the light-deprived rabbits. Decrease in the ratio GABA transaminase/glutamate decarboxylase indicated distinct inhibition of GABA degradation as compared with its synthesis due to absence of sensory impulsation within the early periods of postnatal ontogenesis of the animals."}
{"id": "PMID:473684", "title": "[Identification of the amino acids participating in interaction with DNA in the makeup of a viral nucleoprotein].", "content": "A method is described for identification of amino acids participating in covalent DNA-protein cross-links formed in the course of modification of native CD phage by soldium bisulfite. The method is based on estimation of increased content of free amino acids, formed after treatment of the isolated cross-link products using equimolar mixture of O-methyl hydroxylamine and sodium bisulfite. Thin layer chromatography on the plates Fixion 50 x 8 was used for comparative semi-quantitative analysis of amino acids.", "contents": "[Identification of the amino acids participating in interaction with DNA in the makeup of a viral nucleoprotein]. A method is described for identification of amino acids participating in covalent DNA-protein cross-links formed in the course of modification of native CD phage by soldium bisulfite. The method is based on estimation of increased content of free amino acids, formed after treatment of the isolated cross-link products using equimolar mixture of O-methyl hydroxylamine and sodium bisulfite. Thin layer chromatography on the plates Fixion 50 x 8 was used for comparative semi-quantitative analysis of amino acids."}
{"id": "PMID:473690", "title": "[Cholesterol content in human and rabbit erythrocytes in atherosclerosis and experimental hypercholesteremia].", "content": "Direct correlation was observed between the content of cholesterol in erythrocytes and the level of alimentary hypercholesterolemia as well as the severity of heart ischemic disease in patients. Data on alterations in cholesterol content in erythrocytes may be used as a prognostic test for development of ischemic impairments of heart.", "contents": "[Cholesterol content in human and rabbit erythrocytes in atherosclerosis and experimental hypercholesteremia]. Direct correlation was observed between the content of cholesterol in erythrocytes and the level of alimentary hypercholesterolemia as well as the severity of heart ischemic disease in patients. Data on alterations in cholesterol content in erythrocytes may be used as a prognostic test for development of ischemic impairments of heart."}
{"id": "PMID:473691", "title": "[Interaction of acrylonitrile with the microsomal oxidation system of the rat liver].", "content": "A complex with unusual spectral properties was formed after interaction of acrylonitrile with microsomal cytochrome P-450. In the absorption spectrum of the complex the minimum was observed at 400-410 nm but no distinct absorption maxima were found. Acrylonitrile stimulated utilization of oxygen during oxidation of NADP and inhibited hydroxylation of p-nitroanizol and N-demethylation of dimethylaniline. The data obtained suggest possibility of interaction of acrylonitrile with the microsomal system of oxidation.", "contents": "[Interaction of acrylonitrile with the microsomal oxidation system of the rat liver]. A complex with unusual spectral properties was formed after interaction of acrylonitrile with microsomal cytochrome P-450. In the absorption spectrum of the complex the minimum was observed at 400-410 nm but no distinct absorption maxima were found. Acrylonitrile stimulated utilization of oxygen during oxidation of NADP and inhibited hydroxylation of p-nitroanizol and N-demethylation of dimethylaniline. The data obtained suggest possibility of interaction of acrylonitrile with the microsomal system of oxidation."}
{"id": "PMID:473692", "title": "[Nonpathogenic Neisseria studied for the content of histidine decarboxylase].", "content": "Histidine decarboxylase activity was not found in cells of nonpathogenic neisseria, obtained from bronchi of patients with infectious asthma. The absence of the histidine decarboxylase activity in nonpathogenic neisseria cells suggests that the sensitizing activity of these cells was not caused by a direct effect of histamine of bacterial origin on human branchopulmonary tissues.", "contents": "[Nonpathogenic Neisseria studied for the content of histidine decarboxylase]. Histidine decarboxylase activity was not found in cells of nonpathogenic neisseria, obtained from bronchi of patients with infectious asthma. The absence of the histidine decarboxylase activity in nonpathogenic neisseria cells suggests that the sensitizing activity of these cells was not caused by a direct effect of histamine of bacterial origin on human branchopulmonary tissues."}
{"id": "PMID:473769", "title": "[The evaluation of patients with urinary calculi discloses disturbances of metabolism in 75% of all cases (author's transl)].", "content": "The causes of, and physiopathological factors underlying the most common metabolic disorders implicated in the formation of renal stones are reviewed. These include hypercalciuria, hyperoxaluria, renal tubular acidosis, cystinuria and disturbances of purine metabolism. Apart from metabolic disorders the risk of stone formation is also influenced by a low inhibitor activity in urine. Though some aspects in the pathogenesis of urolithiasis remain uncertain, the exact knowlege of important aetiological factors of stone formation is the basis of correct treatment and the prevention of recurrence of urinary calculi.", "contents": "[The evaluation of patients with urinary calculi discloses disturbances of metabolism in 75% of all cases (author's transl)]. The causes of, and physiopathological factors underlying the most common metabolic disorders implicated in the formation of renal stones are reviewed. These include hypercalciuria, hyperoxaluria, renal tubular acidosis, cystinuria and disturbances of purine metabolism. Apart from metabolic disorders the risk of stone formation is also influenced by a low inhibitor activity in urine. Though some aspects in the pathogenesis of urolithiasis remain uncertain, the exact knowlege of important aetiological factors of stone formation is the basis of correct treatment and the prevention of recurrence of urinary calculi."}
{"id": "PMID:473770", "title": "[Amanita phalloides poisoning in Austria (author's transl)].", "content": "An analysis of 28 cases of amanita phalloides poisoning serves as basis for a discussion of the clinical features and therapeutic problems involved. A critical review of recent experimental investigations in animals points to new possibilities in the treatment of amanita phalloides poisoning.", "contents": "[Amanita phalloides poisoning in Austria (author's transl)]. An analysis of 28 cases of amanita phalloides poisoning serves as basis for a discussion of the clinical features and therapeutic problems involved. A critical review of recent experimental investigations in animals points to new possibilities in the treatment of amanita phalloides poisoning."}
{"id": "PMID:473771", "title": "[Long-term treatment of tuberculosis with isoprodian (author's transl)].", "content": "The results of combination therapy of Isoprodian with ethambul in patients with severe pulmonary tuberculosis were found to be comparable with the results previously obtained by us with other first-choice therapeutic regimens. 61% of the 140 cavities found before treatment disappeared within 5 months and 74% within 8 months of therapy. Disappearance of bacilli from the sputum was demonstrated in 89% of the patients by 12 weeks and in all patients by 20 weeks of treatment. The satisfactory therapeutic results, the low toxicity, the convenience of administration and, moreover, the economic aspect of the relatively lower cost of Isoprodian therapy justify the further use of Isoprodian supplemented with a first-choice tuberculostatic drug in the initial phase of treatment followed by long-term therapy with Isoprodian on its own in the stabilizing phase of even severe cases of tuberculosis.", "contents": "[Long-term treatment of tuberculosis with isoprodian (author's transl)]. The results of combination therapy of Isoprodian with ethambul in patients with severe pulmonary tuberculosis were found to be comparable with the results previously obtained by us with other first-choice therapeutic regimens. 61% of the 140 cavities found before treatment disappeared within 5 months and 74% within 8 months of therapy. Disappearance of bacilli from the sputum was demonstrated in 89% of the patients by 12 weeks and in all patients by 20 weeks of treatment. The satisfactory therapeutic results, the low toxicity, the convenience of administration and, moreover, the economic aspect of the relatively lower cost of Isoprodian therapy justify the further use of Isoprodian supplemented with a first-choice tuberculostatic drug in the initial phase of treatment followed by long-term therapy with Isoprodian on its own in the stabilizing phase of even severe cases of tuberculosis."}
{"id": "PMID:473773", "title": "[Biomedical technique in physiology (author's transl)].", "content": "On the basis of two examples of projects being realized in the department of physiology in Graz the use of engineering methods in physiological research is demonstrated. The first example presents a survey of the use of system-theoretical methods for the investigation of overall regulation of circulation under working conditions. Using two test-signals (impulse input, random input) for experimental identification, the impulse response of heart-rate to changes in work load was obtained. The impulse responses of the volunteers were then characterized by a set of three parameters, allowing statistical evaluation, classification for diagnostic means and verification of hypotheses. The second example deals with an investigation of the signal structure of ultrasound-CW-Doppler-systems. Basing on the general Doppler-principle, which is already in use in angiology it was tried to quantify the determinants of the audio-signal, describing it by statistical functions and by experiments on simple models (tube-models). The goal of the experiments is a quantitative assessment of volume-flow and an enhancement of information about the scattering medium (rheological properties of blood).", "contents": "[Biomedical technique in physiology (author's transl)]. On the basis of two examples of projects being realized in the department of physiology in Graz the use of engineering methods in physiological research is demonstrated. The first example presents a survey of the use of system-theoretical methods for the investigation of overall regulation of circulation under working conditions. Using two test-signals (impulse input, random input) for experimental identification, the impulse response of heart-rate to changes in work load was obtained. The impulse responses of the volunteers were then characterized by a set of three parameters, allowing statistical evaluation, classification for diagnostic means and verification of hypotheses. The second example deals with an investigation of the signal structure of ultrasound-CW-Doppler-systems. Basing on the general Doppler-principle, which is already in use in angiology it was tried to quantify the determinants of the audio-signal, describing it by statistical functions and by experiments on simple models (tube-models). The goal of the experiments is a quantitative assessment of volume-flow and an enhancement of information about the scattering medium (rheological properties of blood)."}
{"id": "PMID:473774", "title": "[Significance and tasks of work physiology (author's transl)].", "content": "In the department of physiology in Graz the projects in the field of work physiology are concerned with basic and applied research. Currently the interest is focussed mainly on problems of exercise physiology. By a system analysis of the cardiorespiratory system the influence of exercise on respiration and circulation is examined. Furthermore routine methods for testing the training condition of athletes are being developed.", "contents": "[Significance and tasks of work physiology (author's transl)]. In the department of physiology in Graz the projects in the field of work physiology are concerned with basic and applied research. Currently the interest is focussed mainly on problems of exercise physiology. By a system analysis of the cardiorespiratory system the influence of exercise on respiration and circulation is examined. Furthermore routine methods for testing the training condition of athletes are being developed."}
{"id": "PMID:473775", "title": "[Investigations concerning the body fluid homeostasis (author's transl)].", "content": "The mechanical oscillator technique is used to investigate venous hemodynamics and body fluid homeostasis. The continuous recording of blood density and its relation to hematocrit, plasma density and red cell density yields information about transcapillary fluid shift phenomena.", "contents": "[Investigations concerning the body fluid homeostasis (author's transl)]. The mechanical oscillator technique is used to investigate venous hemodynamics and body fluid homeostasis. The continuous recording of blood density and its relation to hematocrit, plasma density and red cell density yields information about transcapillary fluid shift phenomena."}
{"id": "PMID:473776", "title": "[Analysis of respiration in the sleeping infant. Possible relationship to sudden infant death].", "content": "Preliminary results are indicating, that disorders of the control of respiration during sleep may be an explanation of the sudden infant death syndrome (SIDS). It should be possible to determine individual risk factors for SIDS by means of improved and extended studies, leading finally to a successful prevention of infant's death.", "contents": "[Analysis of respiration in the sleeping infant. Possible relationship to sudden infant death]. Preliminary results are indicating, that disorders of the control of respiration during sleep may be an explanation of the sudden infant death syndrome (SIDS). It should be possible to determine individual risk factors for SIDS by means of improved and extended studies, leading finally to a successful prevention of infant's death."}
{"id": "PMID:473777", "title": "[Physiology of carbohydrate absorption (author's transl)].", "content": "The only enzymatic mechanism concerning carbohydrates in the chymus is starch decomposition by enzymes in the saliva and pancreatic juice, In contrast to the chymus the enterocytes dispose of all necessary enzymatic activities in their brush border, above all for disaccharide splitting. Furthermore these activities show relatively fast physiological adaptation. The activity of the disaccharidases and the transport of glucose depend on luminal sodium concentration. The transport of glucose and galactose takes place by active mechanisms, the transport of fructose by facilitated diffusion, other monosaccharides are absorbed passively.", "contents": "[Physiology of carbohydrate absorption (author's transl)]. The only enzymatic mechanism concerning carbohydrates in the chymus is starch decomposition by enzymes in the saliva and pancreatic juice, In contrast to the chymus the enterocytes dispose of all necessary enzymatic activities in their brush border, above all for disaccharide splitting. Furthermore these activities show relatively fast physiological adaptation. The activity of the disaccharidases and the transport of glucose depend on luminal sodium concentration. The transport of glucose and galactose takes place by active mechanisms, the transport of fructose by facilitated diffusion, other monosaccharides are absorbed passively."}
{"id": "PMID:473790", "title": "Metabolism of [14C]phenol by sheep, pig and rat.", "content": "1. Following an oral dose of [14C]phenol (12.5 or 25 mg/kg) to sheep, pig and rat, urinary elimination of radioactivity was rapid, 80-90% dose being excreted in the first 8 h. 2. In anaesthetized, ureter-cannulated rats, 70-80% of an intraduodenal dose was eliminated in 2 h; 2% dose was excreted as phenol conjugates in the urine within 10 min. 3. The major urinary metabolites from phenol (25 mg/kg) were phenylglucuronide and phenylsulphate. In the sheep, pig and rat, the glucuronide accounted for 49%, 83% and 42% respectively, of the total urinary metabolites and sulphate accounted for 32%, 1% and 55%. Conjugates of quinol were minor urinary metabolites (less than 7%) in all three species. 4. In sheep some 12% of the urinary metabolites was conjugated with phosphate; this metabolite was not found in rat or pig.", "contents": "Metabolism of [14C]phenol by sheep, pig and rat. 1. Following an oral dose of [14C]phenol (12.5 or 25 mg/kg) to sheep, pig and rat, urinary elimination of radioactivity was rapid, 80-90% dose being excreted in the first 8 h. 2. In anaesthetized, ureter-cannulated rats, 70-80% of an intraduodenal dose was eliminated in 2 h; 2% dose was excreted as phenol conjugates in the urine within 10 min. 3. The major urinary metabolites from phenol (25 mg/kg) were phenylglucuronide and phenylsulphate. In the sheep, pig and rat, the glucuronide accounted for 49%, 83% and 42% respectively, of the total urinary metabolites and sulphate accounted for 32%, 1% and 55%. Conjugates of quinol were minor urinary metabolites (less than 7%) in all three species. 4. In sheep some 12% of the urinary metabolites was conjugated with phosphate; this metabolite was not found in rat or pig."}
{"id": "PMID:473792", "title": "The influences of the route of administration on the metabolism and excretion of bitolterol, a new bronchodilator, in the rat.", "content": "1. [3H]Bitolterol, an ester prodrug to colterol (N-t-butyl-arterenol), when administered orally, was excreted mostly in the urine; approx. equal amounts of 3H were found in urine and faeces after intraperitoneal or intravenous injection. 2. Half the dose was excreted in the bile following parenteral administration, while only a small amount of radioactivity was found in bile after oral dosage. The biliary-excreted material consisted mainly of glucuronides, for all routes of administration. 3. The glucuronides of colterol and 3-O-methyl-colterol were excreted in urine after oral administration of bitolterol. In addition to the glucuronides, free colterol and 3-O-methyl-colterol were excreted in urine following parenteral administration. 4. A part of bitolterol was hydrolysed to colterol in rat stomach, and bitolterol was more rapidly hydrolysed to colteral with homogenates of intestinal mucosa than with stomach homogenates in vitro.", "contents": "The influences of the route of administration on the metabolism and excretion of bitolterol, a new bronchodilator, in the rat. 1. [3H]Bitolterol, an ester prodrug to colterol (N-t-butyl-arterenol), when administered orally, was excreted mostly in the urine; approx. equal amounts of 3H were found in urine and faeces after intraperitoneal or intravenous injection. 2. Half the dose was excreted in the bile following parenteral administration, while only a small amount of radioactivity was found in bile after oral dosage. The biliary-excreted material consisted mainly of glucuronides, for all routes of administration. 3. The glucuronides of colterol and 3-O-methyl-colterol were excreted in urine after oral administration of bitolterol. In addition to the glucuronides, free colterol and 3-O-methyl-colterol were excreted in urine following parenteral administration. 4. A part of bitolterol was hydrolysed to colterol in rat stomach, and bitolterol was more rapidly hydrolysed to colteral with homogenates of intestinal mucosa than with stomach homogenates in vitro."}
{"id": "PMID:473793", "title": "Metabolic fate of noscapine. II. Isolation and identification of novel metabolites produced by C-C bond cleavage.", "content": "1. The metabolism of noscapine was studied in rabbits, rats and human. 2. Apart from the O-demethylated metabolites already reported, three metabolites were isolated and identified as meconine, cotarnine and hydrocotamine, respectively. 3. Mass fragmentography (chemical ionization) was used to determine these products simultaneously. 4. Meconine was the major metabolite in all three species, accounting for about 3, 8 and 2% of the dose in the first 24 h urines of rats, rabbits and humans, respectively, although one woman excreted to a very high extent (22%). In rats, cotarnine bases were excreted at almost same level as that of meconine, but at lesser extent in other two species. Hydrocotamine and unchanged noscapine were excreted to less than 1% dose.", "contents": "Metabolic fate of noscapine. II. Isolation and identification of novel metabolites produced by C-C bond cleavage. 1. The metabolism of noscapine was studied in rabbits, rats and human. 2. Apart from the O-demethylated metabolites already reported, three metabolites were isolated and identified as meconine, cotarnine and hydrocotamine, respectively. 3. Mass fragmentography (chemical ionization) was used to determine these products simultaneously. 4. Meconine was the major metabolite in all three species, accounting for about 3, 8 and 2% of the dose in the first 24 h urines of rats, rabbits and humans, respectively, although one woman excreted to a very high extent (22%). In rats, cotarnine bases were excreted at almost same level as that of meconine, but at lesser extent in other two species. Hydrocotamine and unchanged noscapine were excreted to less than 1% dose."}
{"id": "PMID:473794", "title": "Metabolism of norcocaine, N-hydroxy norcocaine and cocaine-N-oxide in the rat.", "content": "1. The metabolism of [3H]norcocaine, N-hydroxy[3H]norcocaine and cocaine-N-oxide has been investigated in rats after i.v. injection. 2. The biological t 1/2 of norcocaine (dose 2 mg/kg i.v.) in plasma, liver and brain were 0.4, 1.6, 0.5 h, respectively and the compound was not detectable in the central nervous system 6 h after injection. The % dose of norcocaine excreted unchanged in urine and faeces in 96 h were 0.7 and 1.0, respectively. Benzoylnorecgonine, norecgonine, norecgonine methyl ester and an unidentified compound were excreted in urine. 3. The biological t 1/2 of N-hydroxynorcocaine (5 mg/kg i.v.) in brain and plasma were 0.3, 1.6 h respectively and only 1.3 and 1.6% of dose were excreted unchanged in urine and faeces in 96 h. N-Hydroxybenzoylnorecgonine and N-hydroxynorecgonine methyl ester were the major urinary metabolites. N-hydroxynorcocaine was not metabolized to norcocaine in vitro by liver microsomes. Doses of greater than 7.5 mg/kg i.v. resulted in death of rats by cardiorespiratory arrest. 4. Cocaine-N-oxide (50 mg/kg i.v.) yielded ecgonine-N-oxide methyl ester as its major metabolite; other minor metabolites were cocaine (0.5%), norcocaine (1%), benzoylecgonine, ecgonine, ecgonine-N-oxide, along with minor amounts of unmetabolized compound. Lethality of cocaine-N-oxide (100 mg/kg i.v.) was possibly due to metabolism to norcocaine and cocaine.", "contents": "Metabolism of norcocaine, N-hydroxy norcocaine and cocaine-N-oxide in the rat. 1. The metabolism of [3H]norcocaine, N-hydroxy[3H]norcocaine and cocaine-N-oxide has been investigated in rats after i.v. injection. 2. The biological t 1/2 of norcocaine (dose 2 mg/kg i.v.) in plasma, liver and brain were 0.4, 1.6, 0.5 h, respectively and the compound was not detectable in the central nervous system 6 h after injection. The % dose of norcocaine excreted unchanged in urine and faeces in 96 h were 0.7 and 1.0, respectively. Benzoylnorecgonine, norecgonine, norecgonine methyl ester and an unidentified compound were excreted in urine. 3. The biological t 1/2 of N-hydroxynorcocaine (5 mg/kg i.v.) in brain and plasma were 0.3, 1.6 h respectively and only 1.3 and 1.6% of dose were excreted unchanged in urine and faeces in 96 h. N-Hydroxybenzoylnorecgonine and N-hydroxynorecgonine methyl ester were the major urinary metabolites. N-hydroxynorcocaine was not metabolized to norcocaine in vitro by liver microsomes. Doses of greater than 7.5 mg/kg i.v. resulted in death of rats by cardiorespiratory arrest. 4. Cocaine-N-oxide (50 mg/kg i.v.) yielded ecgonine-N-oxide methyl ester as its major metabolite; other minor metabolites were cocaine (0.5%), norcocaine (1%), benzoylecgonine, ecgonine, ecgonine-N-oxide, along with minor amounts of unmetabolized compound. Lethality of cocaine-N-oxide (100 mg/kg i.v.) was possibly due to metabolism to norcocaine and cocaine."}
{"id": "PMID:473812", "title": "[Comparative ultrasonic Doppler blood pressure determination on the upper and lower extremities as a possibility in the early detection and course observation of peripheral arterial circulatory disorders].", "content": "The comparative measuring of blood pressure on the arm and foot artery by means of the ultrasound technique after Doppler is a method for the diagnostics of the disturbance of the peripheral arterial blood supply relevant to practice. Its advantages are in the short time of examination, the simple manipulation, the little technical expenditure and the providing of clear measuring data. The course of examination used by us is explained, the normal and borderline values got are reported. Patients with haemodynamically effective disturbances of the arterial blood supply of the vessels of the leg have on the malleolar artery a by more than 10 Torr lower blood pressure than on the brachial artery. For recognition of early changes we propose a simple loading test in form of a position test. The comparative ultrasound measuring of the blood pressure after Doppler on the upper and lower extremity is suitable for the observation of the course and should stand in the first place of the functional diagnostics of peripheral angioorganopathies.", "contents": "[Comparative ultrasonic Doppler blood pressure determination on the upper and lower extremities as a possibility in the early detection and course observation of peripheral arterial circulatory disorders]. The comparative measuring of blood pressure on the arm and foot artery by means of the ultrasound technique after Doppler is a method for the diagnostics of the disturbance of the peripheral arterial blood supply relevant to practice. Its advantages are in the short time of examination, the simple manipulation, the little technical expenditure and the providing of clear measuring data. The course of examination used by us is explained, the normal and borderline values got are reported. Patients with haemodynamically effective disturbances of the arterial blood supply of the vessels of the leg have on the malleolar artery a by more than 10 Torr lower blood pressure than on the brachial artery. For recognition of early changes we propose a simple loading test in form of a position test. The comparative ultrasound measuring of the blood pressure after Doppler on the upper and lower extremity is suitable for the observation of the course and should stand in the first place of the functional diagnostics of peripheral angioorganopathies."}
{"id": "PMID:473813", "title": "[Simultaneous occurrence of arteriosclerosis obliterans and venous diseases].", "content": "It is reported on the pathophysiological peculiarities which may appear in the coincidence of the two vascular diseases. As therapy the fibrinolysis with Awelysin should always be taken into consideration.", "contents": "[Simultaneous occurrence of arteriosclerosis obliterans and venous diseases]. It is reported on the pathophysiological peculiarities which may appear in the coincidence of the two vascular diseases. As therapy the fibrinolysis with Awelysin should always be taken into consideration."}
{"id": "PMID:473814", "title": "[Etiology and possible prevention of hemostasis-related complications in angiographies with triiodinated contrast media].", "content": "The influence of the triiodinated angiographic contrast medium Visotrast 370 on haemostasis was studied in a total of 30 angiographic examinations with and without previous injection of 5,000 IU heparin. The contrast medium alone caused prolongation of bleeding time and inhibition of plasmatic coagulation. The effect of the contrast medium was enhanced by injection of heparin. The fibrinogen concentration remained unchanged in all cases. Aggregation of platelets enhanced by the contrast medium was found to be further increased by heparin. Enhanced platelet aggregation is considered an essential factor in the incidence of thromboembolic complications during engiographic examinations. Since heparin does not inhibit platalet function, the use of inhibitors of aggregation is discussed.", "contents": "[Etiology and possible prevention of hemostasis-related complications in angiographies with triiodinated contrast media]. The influence of the triiodinated angiographic contrast medium Visotrast 370 on haemostasis was studied in a total of 30 angiographic examinations with and without previous injection of 5,000 IU heparin. The contrast medium alone caused prolongation of bleeding time and inhibition of plasmatic coagulation. The effect of the contrast medium was enhanced by injection of heparin. The fibrinogen concentration remained unchanged in all cases. Aggregation of platelets enhanced by the contrast medium was found to be further increased by heparin. Enhanced platelet aggregation is considered an essential factor in the incidence of thromboembolic complications during engiographic examinations. Since heparin does not inhibit platalet function, the use of inhibitors of aggregation is discussed."}
{"id": "PMID:473815", "title": "[Transvenous electrode implantation into the left heart auricle via vena saphena magna].", "content": "A new simple variant of the transvernous implantation of atrial electrodes for the pacemaker therapy is reported. It consist in the fact that via the V. saphena magna under fluorography a hook electrode is shifted into the right auricular appendix by means of a slightly bent conducting wire and is anchored by leading out the hooks.", "contents": "[Transvenous electrode implantation into the left heart auricle via vena saphena magna]. A new simple variant of the transvernous implantation of atrial electrodes for the pacemaker therapy is reported. It consist in the fact that via the V. saphena magna under fluorography a hook electrode is shifted into the right auricular appendix by means of a slightly bent conducting wire and is anchored by leading out the hooks."}
{"id": "PMID:473816", "title": "[The problem of hypertension and ovulation inhibitors].", "content": "It is reported on a 46-year-old woman who died of a haemorrhage of the cerebral matter in renally fixed hypertension and severe arteriosclerosis of the arteries of the basis of the brain. The possible connection between hypertension, arteriolosclerosis of the kidneys and arterioclerosis of the arteries of the brain basis and an intake of ovulation inhibitors lasting 10 years is critically discussed.", "contents": "[The problem of hypertension and ovulation inhibitors]. It is reported on a 46-year-old woman who died of a haemorrhage of the cerebral matter in renally fixed hypertension and severe arteriosclerosis of the arteries of the basis of the brain. The possible connection between hypertension, arteriolosclerosis of the kidneys and arterioclerosis of the arteries of the brain basis and an intake of ovulation inhibitors lasting 10 years is critically discussed."}
{"id": "PMID:473817", "title": "[Differential diagnosis of interstitial lung diseases].", "content": "It is reported on the different forms of the interstitial pulmonary diseases. Apart from the clinical examination the establishment of the professional and hobby anamnesis play an important part for the differential diagnosis. Allergologic and immunologic examination methods may contribute to the etiologic clarification. In unclear findings a bioptic ascertainment is necessary, though histologically not all cases are to be clarified. The author enters the most important clinical pictures and refers to the difficulties of the radiological differential diagnostics. Questions of therapy are briefly discussed.", "contents": "[Differential diagnosis of interstitial lung diseases]. It is reported on the different forms of the interstitial pulmonary diseases. Apart from the clinical examination the establishment of the professional and hobby anamnesis play an important part for the differential diagnosis. Allergologic and immunologic examination methods may contribute to the etiologic clarification. In unclear findings a bioptic ascertainment is necessary, though histologically not all cases are to be clarified. The author enters the most important clinical pictures and refers to the difficulties of the radiological differential diagnostics. Questions of therapy are briefly discussed."}
{"id": "PMID:473818", "title": "[Differential diagnosis of pleural diseases].", "content": "Diffuse and circular focal processes are radiologically recognized as possibly pleurally conditioned by the position near to the chest wall. A clarification is possible by means of the diagnostic pneumothorax, the pleuroscopy and the exploratory excision under vision. Callous processes may be of inflammatory and malignant genesis. Transthoracic punctures, punch biopsies and surgical exploration may become necessary for the clarification. The effusion is the most frequent symptom of pleural diseases. The differentiation of transudate and exudate is diagnostically as essential as a functional interpretation of the cell sediments of above all inflammatory effusions. Not all malignant processes are cyto-morphologically to be recognized as such. By means of the determination of the protein coagulation after Weltmann, simultaneously performed in the blood serum and in the effusion into the pleura, we, therefore, try to find additional informations. The colorimetric measuring of the contents of hyaluronic acid of pleura exudates serves the identification of diffuse mesotheliomas.", "contents": "[Differential diagnosis of pleural diseases]. Diffuse and circular focal processes are radiologically recognized as possibly pleurally conditioned by the position near to the chest wall. A clarification is possible by means of the diagnostic pneumothorax, the pleuroscopy and the exploratory excision under vision. Callous processes may be of inflammatory and malignant genesis. Transthoracic punctures, punch biopsies and surgical exploration may become necessary for the clarification. The effusion is the most frequent symptom of pleural diseases. The differentiation of transudate and exudate is diagnostically as essential as a functional interpretation of the cell sediments of above all inflammatory effusions. Not all malignant processes are cyto-morphologically to be recognized as such. By means of the determination of the protein coagulation after Weltmann, simultaneously performed in the blood serum and in the effusion into the pleura, we, therefore, try to find additional informations. The colorimetric measuring of the contents of hyaluronic acid of pleura exudates serves the identification of diffuse mesotheliomas."}
{"id": "PMID:473819", "title": "[Cytodiagnosis in lung diseases].", "content": "The main application of the clinical cytology in pulmonary diseases is a possibly early and certain tumour diagnostics. This is successful in 80 to 90% of the cases. The rate of the falsely positive findings is in general lower than 1%, in the own material at 0.5%. When the cytodiagnostician taken into consideration reference pictures to benign diseases and helps his clinical partner in the classification of these findings into the entire clinical pictures, the value of the cytodiagnostics increases manifold. These descriptions were confirmed by cytological pictures and of cases instances.", "contents": "[Cytodiagnosis in lung diseases]. The main application of the clinical cytology in pulmonary diseases is a possibly early and certain tumour diagnostics. This is successful in 80 to 90% of the cases. The rate of the falsely positive findings is in general lower than 1%, in the own material at 0.5%. When the cytodiagnostician taken into consideration reference pictures to benign diseases and helps his clinical partner in the classification of these findings into the entire clinical pictures, the value of the cytodiagnostics increases manifold. These descriptions were confirmed by cytological pictures and of cases instances."}
{"id": "PMID:473820", "title": "[Immunologic diagnosis].", "content": "The development of the immunologic basic research and a deeper insight into the pathogenesis of diseases with changed immune reactivity led to an intensivation of the clinic-immunologic in-vivo- and in-vitro-diagnostics. At the instance of the pulmonary diseases according to the classification of Gell and Coombs the immunologic-diagnostic possibilities and problems are shown. Here is referred to the importance of the use of standardized tests and of the wide replaceability of in-vivo-techniques by in-vitro-methods.", "contents": "[Immunologic diagnosis]. The development of the immunologic basic research and a deeper insight into the pathogenesis of diseases with changed immune reactivity led to an intensivation of the clinic-immunologic in-vivo- and in-vitro-diagnostics. At the instance of the pulmonary diseases according to the classification of Gell and Coombs the immunologic-diagnostic possibilities and problems are shown. Here is referred to the importance of the use of standardized tests and of the wide replaceability of in-vivo-techniques by in-vitro-methods."}
{"id": "PMID:473821", "title": "[Importance of the work environment for the respiratory system].", "content": "In the working process a great number of dusts, gases and smokes affect on the respiratory organs which may lead to fibrogenic, irritative, toxic, cancerogenic or allergic reactions, when the norms of industrial hygiene are not observed. In addition to this come still infective agents. Also communal surroundings, habits of life and medicaments are able to impair the respiratory organ with concurrent noxae. Among the professional diseases which are annually acknowledged about 1,200 (10%) concern the respiratory organ. Apart from the classical professional diseases silicosis and asbestosis unspecific diseases of the respiratory organ, carcinomas and allergoses come into the foreground of interest. The report needs special knowledge in the field of professional medicine and an analysis of the working conditions. The observation of the MAK-values is the prerequisite for the prophylaxis and prevention. For noxae with biologic long-term effects the life-long control of the exposed persons is necessary.", "contents": "[Importance of the work environment for the respiratory system]. In the working process a great number of dusts, gases and smokes affect on the respiratory organs which may lead to fibrogenic, irritative, toxic, cancerogenic or allergic reactions, when the norms of industrial hygiene are not observed. In addition to this come still infective agents. Also communal surroundings, habits of life and medicaments are able to impair the respiratory organ with concurrent noxae. Among the professional diseases which are annually acknowledged about 1,200 (10%) concern the respiratory organ. Apart from the classical professional diseases silicosis and asbestosis unspecific diseases of the respiratory organ, carcinomas and allergoses come into the foreground of interest. The report needs special knowledge in the field of professional medicine and an analysis of the working conditions. The observation of the MAK-values is the prerequisite for the prophylaxis and prevention. For noxae with biologic long-term effects the life-long control of the exposed persons is necessary."}
{"id": "PMID:473823", "title": "[Comparative studies in young hypertensive patients at rest and after standardized physical and sensomotoric exertion].", "content": "The acknowledged role of central-nervous mechanisms in the etiopathogenesis of the essential arterial hypertension led to the use of a psycho-physiologic test method with the aim of the early recognition of stress-induced permanent changes of physiologic functions. We succeed in proving the chosen sensumotoric stress in a group of patients with essential arterial hypertension which at first appears uniform in clinical respect allows a further differentiation. On the basis of the behaviour of defined physiologic and biochemical parameters after physical and sensumotoric stress it is referred to the necessity of a differentiated therapy of the early form of the essential arterial hypertension.", "contents": "[Comparative studies in young hypertensive patients at rest and after standardized physical and sensomotoric exertion]. The acknowledged role of central-nervous mechanisms in the etiopathogenesis of the essential arterial hypertension led to the use of a psycho-physiologic test method with the aim of the early recognition of stress-induced permanent changes of physiologic functions. We succeed in proving the chosen sensumotoric stress in a group of patients with essential arterial hypertension which at first appears uniform in clinical respect allows a further differentiation. On the basis of the behaviour of defined physiologic and biochemical parameters after physical and sensumotoric stress it is referred to the necessity of a differentiated therapy of the early form of the essential arterial hypertension."}
{"id": "PMID:473824", "title": "[Hepatitis prophylaxis in dialysis centers. A list of recommendations and tips. I].", "content": "From the standpoint of prevention of hepatitis in this first report references are given to the construction of dialysis centres, to their apparative equipment with special regard to the artificial kidneys, to the dialysators and to the blood-leading systems. Finally the author deals with the technique of punctures and to the possibilities for avoiding too large blood losses in patients undergoing a chronic dialysis.", "contents": "[Hepatitis prophylaxis in dialysis centers. A list of recommendations and tips. I]. From the standpoint of prevention of hepatitis in this first report references are given to the construction of dialysis centres, to their apparative equipment with special regard to the artificial kidneys, to the dialysators and to the blood-leading systems. Finally the author deals with the technique of punctures and to the possibilities for avoiding too large blood losses in patients undergoing a chronic dialysis."}
{"id": "PMID:473825", "title": "[Doppler-sonographic findings in media sclerosis of the extremities, verified by radiography].", "content": "23 patients were examined who in the ultrasound-Doppler-pressure measurement were evident by too large peripheral systolic measuring values. On the X-ray pictures of the soft tissues of these patients more or less expressed linear and longitudinal calcifications of the vascular wall in the sense of a M\u00f6nckeberg sclerosis of the media were to be proved. Though these changes of the arteries which are to be established in most cases in diabetics of older age do not cause, as a rule, any disturbances of the peripheral blood supply, they are of clinical interest, since they falsify the results of indirect measurements of the pressure (pseudohypertension). An arterial obstructive disease which is simultaneously present may possibly been overseen and the false diagnosis of a therapy-resistant arterial hypertension may be made.", "contents": "[Doppler-sonographic findings in media sclerosis of the extremities, verified by radiography]. 23 patients were examined who in the ultrasound-Doppler-pressure measurement were evident by too large peripheral systolic measuring values. On the X-ray pictures of the soft tissues of these patients more or less expressed linear and longitudinal calcifications of the vascular wall in the sense of a M\u00f6nckeberg sclerosis of the media were to be proved. Though these changes of the arteries which are to be established in most cases in diabetics of older age do not cause, as a rule, any disturbances of the peripheral blood supply, they are of clinical interest, since they falsify the results of indirect measurements of the pressure (pseudohypertension). An arterial obstructive disease which is simultaneously present may possibly been overseen and the false diagnosis of a therapy-resistant arterial hypertension may be made."}
{"id": "PMID:473827", "title": "[Prevention of hepatitis in dialysis centers. A catalog of recommendations and directions. 2].", "content": "On 33 patients who underwent a lung resection under conditions of stress in the majority of cases also after parenchyma-sparing operation a pulmonary hypertension, in about 50% already a slight hypertension in rest could be proved. The increase of the pulmonary pressure correlated with an increase of the resistance of the pulmonary vascular system, of resistance and age and showed tendencies to the increasing postoperative interval.", "contents": "[Prevention of hepatitis in dialysis centers. A catalog of recommendations and directions. 2]. On 33 patients who underwent a lung resection under conditions of stress in the majority of cases also after parenchyma-sparing operation a pulmonary hypertension, in about 50% already a slight hypertension in rest could be proved. The increase of the pulmonary pressure correlated with an increase of the resistance of the pulmonary vascular system, of resistance and age and showed tendencies to the increasing postoperative interval."}
{"id": "PMID:473828", "title": "[Leiomyomatosis of the anal canal].", "content": "The solitary occurrence of a leiomyoma in the anal channel is rarely observed. Still more infrequently is the multiple appearance of such tumours in the anal channel which is to be named leiomyomatosis. On the basis of an own observation concerning the appearance of a relapsing leiomyomatosis of the anal channel in a 66-year-old woman is reported, which hitherto has not yet been described in literature. The clinical and morphological problems of this disease are discussed.", "contents": "[Leiomyomatosis of the anal canal]. The solitary occurrence of a leiomyoma in the anal channel is rarely observed. Still more infrequently is the multiple appearance of such tumours in the anal channel which is to be named leiomyomatosis. On the basis of an own observation concerning the appearance of a relapsing leiomyomatosis of the anal channel in a 66-year-old woman is reported, which hitherto has not yet been described in literature. The clinical and morphological problems of this disease are discussed."}
{"id": "PMID:473829", "title": "[New aspects in the pathologic anatomy of arteriosclerosis obliterans].", "content": "The pathogenetic notion of arteriosclerosis and the clinical syndrome of the arterial obstructive diseases are not identical, but interfere in different points which are discussed in detail. Arteriosclerotic changes must be delimitated from pure age-conditioned changes of the arteries, since the two are sufficiently defined and deviate from each other in important characteristics. Only the so-called fibrous thickening of the intima is still doubtful concerning its classification. For the understanding of the severe obliterating arteriosclerosis the knowledge of the early stages is necessary. The disease begins with focal proliferations of smooth muscle cells and a disturbance of permeability of the arterial endothelium. The two cell forms are discussed more in detail and estimated in its significance for the vascular disease. Particularly the lesion of the endothelium nowadays plays an increasing role as causative factor for the development of an arteriosclerosis, since already simple increases of permeability via and edema of the intima may lead to an accumulation of lipoproteins in the vascular wall, also when the lipid level in blood is not increased. At present in clinical practice often the question of the delimitation of an obliterating arteriosclerosis from other arteriopathies leading to a vascular obstruction arises. Since a part of these diseases is to be diagnosed by biopsies of the arteries, the histological differential diagnosis of the most important inflammatory arterial diseases is discussed.", "contents": "[New aspects in the pathologic anatomy of arteriosclerosis obliterans]. The pathogenetic notion of arteriosclerosis and the clinical syndrome of the arterial obstructive diseases are not identical, but interfere in different points which are discussed in detail. Arteriosclerotic changes must be delimitated from pure age-conditioned changes of the arteries, since the two are sufficiently defined and deviate from each other in important characteristics. Only the so-called fibrous thickening of the intima is still doubtful concerning its classification. For the understanding of the severe obliterating arteriosclerosis the knowledge of the early stages is necessary. The disease begins with focal proliferations of smooth muscle cells and a disturbance of permeability of the arterial endothelium. The two cell forms are discussed more in detail and estimated in its significance for the vascular disease. Particularly the lesion of the endothelium nowadays plays an increasing role as causative factor for the development of an arteriosclerosis, since already simple increases of permeability via and edema of the intima may lead to an accumulation of lipoproteins in the vascular wall, also when the lipid level in blood is not increased. At present in clinical practice often the question of the delimitation of an obliterating arteriosclerosis from other arteriopathies leading to a vascular obstruction arises. Since a part of these diseases is to be diagnosed by biopsies of the arteries, the histological differential diagnosis of the most important inflammatory arterial diseases is discussed."}
{"id": "PMID:473830", "title": "[Modern aspects of the physiopathology of arteriosclerosis obliterans].", "content": "In a shortened survey new points of view concerning the pathophysiology of disturbances of the peripheral arterial blood supply are discussed. Here the author deals in short with the new hypothesis of the atherogenesis, according to which on the basis of a lesion of the endothelium an aggregation of thrombocytes with following stimulation of the smooth muscle cells of the media develops. The haemodynamic changes by vascular stenoses and obstructions with the possibilities of their recognition are discussed in detail, in which especially examinations under load and phasic measurements of flow are taken into consideration.", "contents": "[Modern aspects of the physiopathology of arteriosclerosis obliterans]. In a shortened survey new points of view concerning the pathophysiology of disturbances of the peripheral arterial blood supply are discussed. Here the author deals in short with the new hypothesis of the atherogenesis, according to which on the basis of a lesion of the endothelium an aggregation of thrombocytes with following stimulation of the smooth muscle cells of the media develops. The haemodynamic changes by vascular stenoses and obstructions with the possibilities of their recognition are discussed in detail, in which especially examinations under load and phasic measurements of flow are taken into consideration."}
{"id": "PMID:473831", "title": "[Modern angiological diagnostic methods].", "content": "The tasks of apparative examination methods in the angiologic diagnostics are explained. Apart from the mechanical oscillography the possibilities of registration and evaluation of the peripheral pulse wave are discussed. As quantitative examination method the plethysmography is broadly used. The most important applications of the ultrasound-Doppler-technique are described.", "contents": "[Modern angiological diagnostic methods]. The tasks of apparative examination methods in the angiologic diagnostics are explained. Apart from the mechanical oscillography the possibilities of registration and evaluation of the peripheral pulse wave are discussed. As quantitative examination method the plethysmography is broadly used. The most important applications of the ultrasound-Doppler-technique are described."}
{"id": "PMID:473832", "title": "[Hemodynamics of the liver. I. Liver perfusion after ligation of the dog hepatic artery and portal vein].", "content": "By means of direct method hepatic blood supply was examined after ligation of hepatic artery and portal vein. 15 per cent of the total blood supply to the liver were arterial. This amount can rise to 30 per cent under extreme conditions, caused by an increase of arteriovenous pressure difference as well as by a decrease of the arterial flow resistance in the liver.", "contents": "[Hemodynamics of the liver. I. Liver perfusion after ligation of the dog hepatic artery and portal vein]. By means of direct method hepatic blood supply was examined after ligation of hepatic artery and portal vein. 15 per cent of the total blood supply to the liver were arterial. This amount can rise to 30 per cent under extreme conditions, caused by an increase of arteriovenous pressure difference as well as by a decrease of the arterial flow resistance in the liver."}
{"id": "PMID:473833", "title": "[Hemodynamics of the liver. II. Hemodynamics of the dog liver after resection of 70% of the liver and ligation of the left branch of the portal vein].", "content": "Blood supply to the liver was determined by help of the direct method after 70 per cent hepatic resection and after ligation of the left branch of portal vein in dogs. The total blood supply remains unchanged after both surgical procedures, while the circulation in the remaining lobes, resp. in those patent for portal blood stream, considerably increases. After the resection portal hypertension develops. This elevated portal pressure was not observed after ligation of the left portal branch.", "contents": "[Hemodynamics of the liver. II. Hemodynamics of the dog liver after resection of 70% of the liver and ligation of the left branch of the portal vein]. Blood supply to the liver was determined by help of the direct method after 70 per cent hepatic resection and after ligation of the left branch of portal vein in dogs. The total blood supply remains unchanged after both surgical procedures, while the circulation in the remaining lobes, resp. in those patent for portal blood stream, considerably increases. After the resection portal hypertension develops. This elevated portal pressure was not observed after ligation of the left portal branch."}
{"id": "PMID:473834", "title": "[Hemodynamics of the liver. III. The importance of the hepatic artery for intrahepatic circulation].", "content": "Values of intrahepatic arterial pressure gradients are presented on a table, based on own hepatic circulatory measurements and own anatomic studies. The importance of the peribiliar net for hemodynamics is stressed, which has regulative properties steering the pressures under which arterial blood gets into the sinusoid capillaries.", "contents": "[Hemodynamics of the liver. III. The importance of the hepatic artery for intrahepatic circulation]. Values of intrahepatic arterial pressure gradients are presented on a table, based on own hepatic circulatory measurements and own anatomic studies. The importance of the peribiliar net for hemodynamics is stressed, which has regulative properties steering the pressures under which arterial blood gets into the sinusoid capillaries."}
{"id": "PMID:473835", "title": "[Critical considerations on the production of a pulsating fluid stream].", "content": "Two types of gears are used for perfusion of cadaveric kidneys. Electric models are presented for both types and the optimal devices are discussed. Based upon in vivo conditions, the relevant parameters are transferred to the model and an according equipment for pulsatile fall-stream perfusion is presented, which is compared with another gear. The clinical criterion for good initial perfusion is the erythrocyte count in the initial perfusate. Judged from that, the chosen device is significantly better than its predecessor.", "contents": "[Critical considerations on the production of a pulsating fluid stream]. Two types of gears are used for perfusion of cadaveric kidneys. Electric models are presented for both types and the optimal devices are discussed. Based upon in vivo conditions, the relevant parameters are transferred to the model and an according equipment for pulsatile fall-stream perfusion is presented, which is compared with another gear. The clinical criterion for good initial perfusion is the erythrocyte count in the initial perfusate. Judged from that, the chosen device is significantly better than its predecessor."}
{"id": "PMID:473836", "title": "The glucoregulatory hormones in cirrhosis of the liver.", "content": "Cirrhosis is complicated by numerous abnormalities of carbohydrate metabolism although these are seldom of clinical importance. Carbohydrate intolerance is extremely common and is accompanied by hyperinsulinaemia, hyperglucagonaemia and elevated levels of gluconeogenic precursors. The hyperinsulinaemia results from impaired hepatic degradation of insulin while recent evidence suggests that pancreatic hypersecretion is responsible for the elevated levels of glucagon in cirrhosis. The role of hepatocellular damage and portal-systemic shunting in the pathogenesis of these abnormalities is controversial but the derangements in carbohydrate metabolism probably reflect hepatocellular damage rather than portal-systemic shunting.", "contents": "The glucoregulatory hormones in cirrhosis of the liver. Cirrhosis is complicated by numerous abnormalities of carbohydrate metabolism although these are seldom of clinical importance. Carbohydrate intolerance is extremely common and is accompanied by hyperinsulinaemia, hyperglucagonaemia and elevated levels of gluconeogenic precursors. The hyperinsulinaemia results from impaired hepatic degradation of insulin while recent evidence suggests that pancreatic hypersecretion is responsible for the elevated levels of glucagon in cirrhosis. The role of hepatocellular damage and portal-systemic shunting in the pathogenesis of these abnormalities is controversial but the derangements in carbohydrate metabolism probably reflect hepatocellular damage rather than portal-systemic shunting."}
{"id": "PMID:473837", "title": "[Treatment of recurrent herpes simplex and recurring stomatitis with levamisol].", "content": "The therapeutic effect of levamisole was tested both in double blind and open clinical trials in all together 32 patients with recurrent herpes simplex and 17 patients was recurrent aphthoses. The frequency, the duration and the painfulness of the herpes eruptions were improved significantly under levamisole treatment, while there was only a short lasting favorable influence on the clinical course of the recurrent aphthoses.", "contents": "[Treatment of recurrent herpes simplex and recurring stomatitis with levamisol]. The therapeutic effect of levamisole was tested both in double blind and open clinical trials in all together 32 patients with recurrent herpes simplex and 17 patients was recurrent aphthoses. The frequency, the duration and the painfulness of the herpes eruptions were improved significantly under levamisole treatment, while there was only a short lasting favorable influence on the clinical course of the recurrent aphthoses."}
{"id": "PMID:473838", "title": "[Levamisol therapy in chronic prostatistis and epididymitis].", "content": "Eight patients suffering from chronic epididymitis, prostatis and urethritis were treated with levamisole. A definite and lasting improvement was seen six of the eight patients.", "contents": "[Levamisol therapy in chronic prostatistis and epididymitis]. Eight patients suffering from chronic epididymitis, prostatis and urethritis were treated with levamisole. A definite and lasting improvement was seen six of the eight patients."}
{"id": "PMID:473839", "title": "[Contribution to the DNCB therapy of alopecia areata].", "content": "14 patients suffering from longstanding alopecia areata totalis or fere totalis were treated with dinitrochlorobenzene (DNCB) over a period of 2 to 11 months. Hair growth was induced in 9 patients. Side effects were not observed so far.", "contents": "[Contribution to the DNCB therapy of alopecia areata]. 14 patients suffering from longstanding alopecia areata totalis or fere totalis were treated with dinitrochlorobenzene (DNCB) over a period of 2 to 11 months. Hair growth was induced in 9 patients. Side effects were not observed so far."}
{"id": "PMID:473840", "title": "[Adjuvant DNCB immunotherapy in malignant melanoma].", "content": "Report on the application of a dinitrochlorobenzene ointment of 61 postoperative melanoma patients exhibiting clinical stages I and II. After contact sensitization the erythemogenic threshold concentrations of DNCB were mostly found in the range of 0,05% and 0,1%. Patients with reactions at low concentrations of 0,01% and 0,05% DNCB were in the mean 8 years younger than those with reactions at 0,1% and 0,5%, but no connection to different stages of malignant melanoma could be evaluated. 3 melanoma patients suffering from skin metastases were treated by epifocal DNCB-application. One of them became clinically tumor free since more than 1 year, whereas the two other exhibiting multicentric and/or profound tumor growth did not respond. In a 82-year-old wife a superficial lentigo maligna melanoma disappeared by DNCB-application. In none of the 61 cases we observed a \"tumor enhancement\" after immunoprophylaxis or adjuvant immunotherapy with DNCB. The DNCB-method in malignant melanoma is yet in the experimental stage and is not recommended for general use in practice.", "contents": "[Adjuvant DNCB immunotherapy in malignant melanoma]. Report on the application of a dinitrochlorobenzene ointment of 61 postoperative melanoma patients exhibiting clinical stages I and II. After contact sensitization the erythemogenic threshold concentrations of DNCB were mostly found in the range of 0,05% and 0,1%. Patients with reactions at low concentrations of 0,01% and 0,05% DNCB were in the mean 8 years younger than those with reactions at 0,1% and 0,5%, but no connection to different stages of malignant melanoma could be evaluated. 3 melanoma patients suffering from skin metastases were treated by epifocal DNCB-application. One of them became clinically tumor free since more than 1 year, whereas the two other exhibiting multicentric and/or profound tumor growth did not respond. In a 82-year-old wife a superficial lentigo maligna melanoma disappeared by DNCB-application. In none of the 61 cases we observed a \"tumor enhancement\" after immunoprophylaxis or adjuvant immunotherapy with DNCB. The DNCB-method in malignant melanoma is yet in the experimental stage and is not recommended for general use in practice."}
{"id": "PMID:473845", "title": "[Effects of acute beta-adrenoceptor blockage (metoprolol i.v.) on plasma norepinephrine concentration and hemodynamics in postmyocardial infarction patients].", "content": "The effect of acute beta-adrenoceptor blockage (Metoprolol) (M), 0.1 mg/kg i.v.) on left ventricular performance has been investigated at rest and during exercise in 15 patients with 2--3 months old transmural myocardial infarctions. Coronary venous and arterial norepinephrine (NE) concentrations were determined. There was no significant change in arterial and coronary venous NE concentrations (0.27 and 0.22 ng/ml, respectively) after blockage of beta-adrenoceptors (0.36 vs 0.26 ng/ml), which caused a fall of stroke volume from 79 to 68 ml, a reduction of ejection fraction from 62 to 55% and of circumferential fibre shortening form 1.2 to 0.9 circ/sec. During physical exercise the plasma NE concentration in the arterial (0.51 ng/ml) and coronary venous (0.6 ng/ml) blood increased significantly and increased even further to 0.65 and 0.76 ng/ml, respectively, following administration of Metoprolol. The arterio-coronary sinus difference in NE concentrations demonstrate a release of NE from the myocardium. As compared to control values, heart rate following Metoprolol was lower (116 vs 106/min), mean PCV pressure was slightly increased (from 21 to 23 mm Hg) and there was a fall of cardiac index from 6.3 to 5.2 l/min X m2. It is likely that the increased sympathetic activity after Metoprolol and during exercise is a compensatory reaction due to the hemodynamic effects of blockade of beta-adrenoceptors. Further studies are in preparation in order to find out if this is only a transient phenomenon during the early adaptation phase after blockade of beta-adrenoceptors.", "contents": "[Effects of acute beta-adrenoceptor blockage (metoprolol i.v.) on plasma norepinephrine concentration and hemodynamics in postmyocardial infarction patients]. The effect of acute beta-adrenoceptor blockage (Metoprolol) (M), 0.1 mg/kg i.v.) on left ventricular performance has been investigated at rest and during exercise in 15 patients with 2--3 months old transmural myocardial infarctions. Coronary venous and arterial norepinephrine (NE) concentrations were determined. There was no significant change in arterial and coronary venous NE concentrations (0.27 and 0.22 ng/ml, respectively) after blockage of beta-adrenoceptors (0.36 vs 0.26 ng/ml), which caused a fall of stroke volume from 79 to 68 ml, a reduction of ejection fraction from 62 to 55% and of circumferential fibre shortening form 1.2 to 0.9 circ/sec. During physical exercise the plasma NE concentration in the arterial (0.51 ng/ml) and coronary venous (0.6 ng/ml) blood increased significantly and increased even further to 0.65 and 0.76 ng/ml, respectively, following administration of Metoprolol. The arterio-coronary sinus difference in NE concentrations demonstrate a release of NE from the myocardium. As compared to control values, heart rate following Metoprolol was lower (116 vs 106/min), mean PCV pressure was slightly increased (from 21 to 23 mm Hg) and there was a fall of cardiac index from 6.3 to 5.2 l/min X m2. It is likely that the increased sympathetic activity after Metoprolol and during exercise is a compensatory reaction due to the hemodynamic effects of blockade of beta-adrenoceptors. Further studies are in preparation in order to find out if this is only a transient phenomenon during the early adaptation phase after blockade of beta-adrenoceptors."}
{"id": "PMID:473846", "title": "[Long-time follow-up scintigraphy with 99m Tc pyrophosphate after myocardial infarction (author's transl)].", "content": "Follow-up scintigraphies with 99m Tc pyrophosphate 3--4 weeks and 6--12 months after a myocardial infarction revealed the possibility of persisting a myocardial tracer activity in cases in which reinfarction can be excluded. There was a relation between the persistence of the tracer activity and the pressure in the pulmonary artery under stress conditions. The diastolic pulmonary pressure was regular in those patients whose scintiphotos showed no tracer activity in the myocardial area in the follow-up scintigraphy. Patients who showed a persisting tracer activity in the infarcted areal had elevated pressures in the pulmonary artery under stress conditions or even at rest. The elevation of the diastolic pulmonary pressure is a sign of an elevated enddiastolic pressure in the left ventricle caused by a limited left ventricular function. This could be proved by left ventriculography. The results in follow-up scintigraphy 3--4 weeks and 6--12 months after the infarction were quite similar. Therefore we believe that the results of a follow-up scintigraphy 3--4 weeks after an infarction allows to draw prognostic inferences about the further course of the disease.", "contents": "[Long-time follow-up scintigraphy with 99m Tc pyrophosphate after myocardial infarction (author's transl)]. Follow-up scintigraphies with 99m Tc pyrophosphate 3--4 weeks and 6--12 months after a myocardial infarction revealed the possibility of persisting a myocardial tracer activity in cases in which reinfarction can be excluded. There was a relation between the persistence of the tracer activity and the pressure in the pulmonary artery under stress conditions. The diastolic pulmonary pressure was regular in those patients whose scintiphotos showed no tracer activity in the myocardial area in the follow-up scintigraphy. Patients who showed a persisting tracer activity in the infarcted areal had elevated pressures in the pulmonary artery under stress conditions or even at rest. The elevation of the diastolic pulmonary pressure is a sign of an elevated enddiastolic pressure in the left ventricle caused by a limited left ventricular function. This could be proved by left ventriculography. The results in follow-up scintigraphy 3--4 weeks and 6--12 months after the infarction were quite similar. Therefore we believe that the results of a follow-up scintigraphy 3--4 weeks after an infarction allows to draw prognostic inferences about the further course of the disease."}
{"id": "PMID:473847", "title": "[On diagnosis of myocardial infarction in the polytraumatized investigation in dogs (author's transl)].", "content": "The reliability of CKMB in cases in which damage of sceletal muscles and myocardium occurred in close sequence is tested in dogs. Results show that in simultaneous damage of heart and muscles, CKMB exceeds the requested ratio of 5 to 8% of total CK, thus proving the myocardial infarction. In cases in which myocardial infarction succeeds a damage of sceletal muscle--e.g. a polytrauma --within distance of hours only, the absolutely high level of CKMB proves the infarction, even below the critical ratio of the total CK.", "contents": "[On diagnosis of myocardial infarction in the polytraumatized investigation in dogs (author's transl)]. The reliability of CKMB in cases in which damage of sceletal muscles and myocardium occurred in close sequence is tested in dogs. Results show that in simultaneous damage of heart and muscles, CKMB exceeds the requested ratio of 5 to 8% of total CK, thus proving the myocardial infarction. In cases in which myocardial infarction succeeds a damage of sceletal muscle--e.g. a polytrauma --within distance of hours only, the absolutely high level of CKMB proves the infarction, even below the critical ratio of the total CK."}
{"id": "PMID:473848", "title": "[The applicability of systolic time intervals in patients before and after aortic valve replacement (author's transl)].", "content": "In order to evaluate the systolic time intervals (STI) for characterizing the left ventricular pump function and the clinical course in patients with aortic valve disease, 35 patients with aortic stenosis (AS) and 25 patients with aortic insufficiency (AI class III or IV (NYHA) were studied before and 1 year after valve replacement either with Bj\u00f6rk-Shiley, Lillehie-Kaster or Starr-Edwards aortic prostheses. Left ventricular ejection time index (LVETI), preejection period index (PEPI) and PEP/LVET were determined and compared with cardiac index (CI), stroke volume index (SVI) and ejection fraction (EF). Prosthetic valve replacement leads to a high significant decrease of LVETI from preop. 468 +/- 31 ms to postop. 394 +/- 26 ms (mean normal values 415 ms) and an increase of PEPI from 100 +/- 26 ms to 136 +/- 25 ms (normal 132 ms) as of PEP/LVET from 0.21 +/- 0.09 to 0.41 +/- 0.12 (normal 0.31). Pre- as well as postop. there is no difference in STI between AS and AI. Also the groups with the three prosthetic valve types do not differ significantly. LVETI and PEPI correlate only postop. with CI and SVI, but PEP/LVET correlates well with EF (r = -0.73) before and after operation. The comparison of STI with the hemodynamic data suggest that preop. values of STI near normal and postop. LVETI below, PEPI and PEP/LVET above the mean values of the whole group with aortic valve replacement indicate left ventricular pump dysfunction. The STI are helpful in the pre-/postop. follow-op of patients with severe aortic valve disease to characterize the clinical and hemodynamic course.", "contents": "[The applicability of systolic time intervals in patients before and after aortic valve replacement (author's transl)]. In order to evaluate the systolic time intervals (STI) for characterizing the left ventricular pump function and the clinical course in patients with aortic valve disease, 35 patients with aortic stenosis (AS) and 25 patients with aortic insufficiency (AI class III or IV (NYHA) were studied before and 1 year after valve replacement either with Bj\u00f6rk-Shiley, Lillehie-Kaster or Starr-Edwards aortic prostheses. Left ventricular ejection time index (LVETI), preejection period index (PEPI) and PEP/LVET were determined and compared with cardiac index (CI), stroke volume index (SVI) and ejection fraction (EF). Prosthetic valve replacement leads to a high significant decrease of LVETI from preop. 468 +/- 31 ms to postop. 394 +/- 26 ms (mean normal values 415 ms) and an increase of PEPI from 100 +/- 26 ms to 136 +/- 25 ms (normal 132 ms) as of PEP/LVET from 0.21 +/- 0.09 to 0.41 +/- 0.12 (normal 0.31). Pre- as well as postop. there is no difference in STI between AS and AI. Also the groups with the three prosthetic valve types do not differ significantly. LVETI and PEPI correlate only postop. with CI and SVI, but PEP/LVET correlates well with EF (r = -0.73) before and after operation. The comparison of STI with the hemodynamic data suggest that preop. values of STI near normal and postop. LVETI below, PEPI and PEP/LVET above the mean values of the whole group with aortic valve replacement indicate left ventricular pump dysfunction. The STI are helpful in the pre-/postop. follow-op of patients with severe aortic valve disease to characterize the clinical and hemodynamic course."}
{"id": "PMID:473849", "title": "[The right ventricle at rest and during exercise. I. Pump-function of the normal right ventricle (author's transl)].", "content": "Right ventricular volumes and function were assessed by biplane cincventriculography at rest and during exercise in 7 normals. Simultaneously pressure in the left ventricle was measured. 1. Stroke volume index of the right ventricle increased from (x +/- SEM) 46.8 +/- R.R ml/m2 at rest to 66.8 +/- 5.5 ml/m2 (p less than .05) during exercise. Ejection fraction increased from 56.4 +/- 3 to 70.3 +/- 3.7% (p less than .02). Enddiastolic volume increased from 82 +/- 5.8 to 95 +/- 7 ml/m2. Enddiastolic right ventricular pressure increased from 5. +/- .8 to 8 +/- 1 mm Hg (p less than .05). Increase of R.V. stroke volume during exercise is achieved at least in part by increased fiber stretch. 2. Endsystolic volume decreased from 36 +/- 3.2 to 28 +/- 4 ml/m2 (p less than .02). Right ventricular mean velocity of diameter shortening (VD) increased from 1.2 +/- .08 to 1.65 +/- .14 D/s (p less than .05). Decrease of endsystolic volume may be caused by decrease of afterload or increase of contractility. Our data do not enable evaluation of these mechanisms.", "contents": "[The right ventricle at rest and during exercise. I. Pump-function of the normal right ventricle (author's transl)]. Right ventricular volumes and function were assessed by biplane cincventriculography at rest and during exercise in 7 normals. Simultaneously pressure in the left ventricle was measured. 1. Stroke volume index of the right ventricle increased from (x +/- SEM) 46.8 +/- R.R ml/m2 at rest to 66.8 +/- 5.5 ml/m2 (p less than .05) during exercise. Ejection fraction increased from 56.4 +/- 3 to 70.3 +/- 3.7% (p less than .02). Enddiastolic volume increased from 82 +/- 5.8 to 95 +/- 7 ml/m2. Enddiastolic right ventricular pressure increased from 5. +/- .8 to 8 +/- 1 mm Hg (p less than .05). Increase of R.V. stroke volume during exercise is achieved at least in part by increased fiber stretch. 2. Endsystolic volume decreased from 36 +/- 3.2 to 28 +/- 4 ml/m2 (p less than .02). Right ventricular mean velocity of diameter shortening (VD) increased from 1.2 +/- .08 to 1.65 +/- .14 D/s (p less than .05). Decrease of endsystolic volume may be caused by decrease of afterload or increase of contractility. Our data do not enable evaluation of these mechanisms."}
{"id": "PMID:473850", "title": "Assessment of regurgitation fractions by radionuclide ventriculography.", "content": "The ratio A for left ventricular and right ventricular enddiastolic-endsystolic count differences was used for quantitative evaluation of aortic and mitral valve regurgitation. The mean value of A for patients without valvular insufficiencies was determined (Am = 1.43 +/- 0.27).23 (96%) of 24 patients with aortic or mitral valve regurgitation showed significantly increased A values (A greater than 2). The numerical value was used for classification of the degree of insufficiency and for comparison with angiography. The correlation between A and the average angiographic degree of insufficiency was 0.79.", "contents": "Assessment of regurgitation fractions by radionuclide ventriculography. The ratio A for left ventricular and right ventricular enddiastolic-endsystolic count differences was used for quantitative evaluation of aortic and mitral valve regurgitation. The mean value of A for patients without valvular insufficiencies was determined (Am = 1.43 +/- 0.27).23 (96%) of 24 patients with aortic or mitral valve regurgitation showed significantly increased A values (A greater than 2). The numerical value was used for classification of the degree of insufficiency and for comparison with angiography. The correlation between A and the average angiographic degree of insufficiency was 0.79."}
{"id": "PMID:473851", "title": "[Echocardiographic representation of the fetal heart (time-motion registration) (author's transl)].", "content": "With time-motion registration of the fetal heart it is possible to get information about the size of the ventricles, the left atrium and the aorta. First results of this method of investigation are demonstrated.", "contents": "[Echocardiographic representation of the fetal heart (time-motion registration) (author's transl)]. With time-motion registration of the fetal heart it is possible to get information about the size of the ventricles, the left atrium and the aorta. First results of this method of investigation are demonstrated."}
{"id": "PMID:473852", "title": "[Echocardiographic investigation of the left ventricular outflow tract in healthy children (author's transl)].", "content": "The width of the left ventricular outflow tract was measured in the echocardiograms at the beginning and the end of the systole in 80 healthy children ranging from 3 to 16 years. The values were referred to the body surface area and the heart volume which was estimated by mean of X-ray. The regression lines as well as the confidence levels of 95 per cent are marked. It becomes obvious that the dispersion of the data related to the heart volume is less than that related to the body surface area. The width of the left ventricular outflow tract with reference to the heart volume and the body surface area resp. shows in relation to the heart volume and the body surface area resp. an exponential behaviour, i.e. in the growing heart the outflow tract decreases in relation to the two parameters. The narrowing of the outflow tract from start to end of the systole is about one third.", "contents": "[Echocardiographic investigation of the left ventricular outflow tract in healthy children (author's transl)]. The width of the left ventricular outflow tract was measured in the echocardiograms at the beginning and the end of the systole in 80 healthy children ranging from 3 to 16 years. The values were referred to the body surface area and the heart volume which was estimated by mean of X-ray. The regression lines as well as the confidence levels of 95 per cent are marked. It becomes obvious that the dispersion of the data related to the heart volume is less than that related to the body surface area. The width of the left ventricular outflow tract with reference to the heart volume and the body surface area resp. shows in relation to the heart volume and the body surface area resp. an exponential behaviour, i.e. in the growing heart the outflow tract decreases in relation to the two parameters. The narrowing of the outflow tract from start to end of the systole is about one third."}
{"id": "PMID:473853", "title": "[The influence of a long-term cardioselective and noncardioselective beta-receptorblockade on blood pressure, O2-uptake and carbohydrate metabolism. Ergometric investigations in hypertensive patients (author's transl)].", "content": "1. In 9 male hypertensives the influence of a long-term cardioselective (200 mg Metoprolol) and non-cardioselective (15 mg Pindolol) beta-receptor blockade on the blood pressure, O2-uptake and carbohydrate metabolism was investigated at rest and during a short (6 min, 100 Watt), long (30 min under steady-state conditions with a heart rate of 130/min) and maximal ergometric work and 5 min after that. 2. In this cross-over trial the blood pressure was reduced significantly (p less than 0.01) and in an identical manner by both beta-receptor blockers. 3. Though there was a significant (p less than 0.01) reduction of the heart rate of 26% by Metoprolol (M) and of 21.2% by Pindolol (P), the O2-uptake during submaximal work was unchanged under P with 1.66 +/- 0.4 l/min and under M with 1.7 +/- 0.44 l/min in comparison with the control (C) of 1.71 +/- 0.38 l/min. The maximal O2-uptake was insignificantly reduced by Pincolol (C 2.51 l/min; M 2.46 l/min; P 2.11 l/min). 4. Only under Pindolol we found in all patients a marked and insignificant (p less than 0.01) drop of the blood sugar level in the 30th min of ergometric work (C 76.2 +/- 11; M 72.6 +/- 9; P 55.3 +/- 11 mg/dl), under maximal work (C 74 +/- 12; M 73.2 +/- 10; P 54.2 +/- 11 mg/dl) and 5 min after (C 83.6 +/- 11; M 87.6 +/- 15;  69 +/- 19 mg/dl). 5. The lactic acid level showed under both beta-receptor blockers the same tendency to decrease. 6. The drop of the blood sugar to hypoglycemic values is due to an impaired glycogenolysis in the skeletal muscles and implies a considerable reduction of the physical capacity. Therefore the cardioselective beta-receptor blocker Metoprolol is recommended especially for the treatment of young hypertensives and for all patients who need their physical fitness in order to achieve their work or to perform a preventive and rehabilitative training.", "contents": "[The influence of a long-term cardioselective and noncardioselective beta-receptorblockade on blood pressure, O2-uptake and carbohydrate metabolism. Ergometric investigations in hypertensive patients (author's transl)]. 1. In 9 male hypertensives the influence of a long-term cardioselective (200 mg Metoprolol) and non-cardioselective (15 mg Pindolol) beta-receptor blockade on the blood pressure, O2-uptake and carbohydrate metabolism was investigated at rest and during a short (6 min, 100 Watt), long (30 min under steady-state conditions with a heart rate of 130/min) and maximal ergometric work and 5 min after that. 2. In this cross-over trial the blood pressure was reduced significantly (p less than 0.01) and in an identical manner by both beta-receptor blockers. 3. Though there was a significant (p less than 0.01) reduction of the heart rate of 26% by Metoprolol (M) and of 21.2% by Pindolol (P), the O2-uptake during submaximal work was unchanged under P with 1.66 +/- 0.4 l/min and under M with 1.7 +/- 0.44 l/min in comparison with the control (C) of 1.71 +/- 0.38 l/min. The maximal O2-uptake was insignificantly reduced by Pincolol (C 2.51 l/min; M 2.46 l/min; P 2.11 l/min). 4. Only under Pindolol we found in all patients a marked and insignificant (p less than 0.01) drop of the blood sugar level in the 30th min of ergometric work (C 76.2 +/- 11; M 72.6 +/- 9; P 55.3 +/- 11 mg/dl), under maximal work (C 74 +/- 12; M 73.2 +/- 10; P 54.2 +/- 11 mg/dl) and 5 min after (C 83.6 +/- 11; M 87.6 +/- 15;  69 +/- 19 mg/dl). 5. The lactic acid level showed under both beta-receptor blockers the same tendency to decrease. 6. The drop of the blood sugar to hypoglycemic values is due to an impaired glycogenolysis in the skeletal muscles and implies a considerable reduction of the physical capacity. Therefore the cardioselective beta-receptor blocker Metoprolol is recommended especially for the treatment of young hypertensives and for all patients who need their physical fitness in order to achieve their work or to perform a preventive and rehabilitative training."}
{"id": "PMID:473854", "title": "[Postnatal development of the median eminence in the rat].", "content": "The postnatal development of the zonation and the glial cells of the median eminence (EM) was investigated in male rats. On the 1st day of life, the ME is mostly undifferentiated. In contrast to adult animals, only the ependymal, subependymal and palisade layer can be observed. The remaining area is almost unorganized. A more distinct zonation of the EM exists about the 10th day of life. Ependymal and subependymal layer are reduced to a monolayer of cells; in addition, the reticular layer appears. The adult structure of the EM is seen for the first time about the 14th day of life. The development of the zonation of the EM is finished at the 3rd week of life. Mature microglial cells occur in the median eminence at the 1st day of live. Ependymal cells, tanycytes and astrocytic tanycytes (Zaborszky and Schiebler 1978), which can be found in a greatter amount as an individual cell type, are relatively immature. Protoplasmatic astrocytes occur at the 4th, and fibrous astrocytes at the 14th day of life. The development of glial cells may be finished at the end of the 4th week of life together with the maturation of oligodendrocytes. The opening of the 3rd ventricle needs 2 weeks, that of the recessus infundibularis is finished after the 3rd week of live. On the basis of their lipid granules and the synaptic contacts with neurosecretory axons it is presumed that the tanycytic and astrocytic tanycytes have regulatory functions in connection with neurosecretory processes.", "contents": "[Postnatal development of the median eminence in the rat]. The postnatal development of the zonation and the glial cells of the median eminence (EM) was investigated in male rats. On the 1st day of life, the ME is mostly undifferentiated. In contrast to adult animals, only the ependymal, subependymal and palisade layer can be observed. The remaining area is almost unorganized. A more distinct zonation of the EM exists about the 10th day of life. Ependymal and subependymal layer are reduced to a monolayer of cells; in addition, the reticular layer appears. The adult structure of the EM is seen for the first time about the 14th day of life. The development of the zonation of the EM is finished at the 3rd week of life. Mature microglial cells occur in the median eminence at the 1st day of live. Ependymal cells, tanycytes and astrocytic tanycytes (Zaborszky and Schiebler 1978), which can be found in a greatter amount as an individual cell type, are relatively immature. Protoplasmatic astrocytes occur at the 4th, and fibrous astrocytes at the 14th day of life. The development of glial cells may be finished at the end of the 4th week of life together with the maturation of oligodendrocytes. The opening of the 3rd ventricle needs 2 weeks, that of the recessus infundibularis is finished after the 3rd week of live. On the basis of their lipid granules and the synaptic contacts with neurosecretory axons it is presumed that the tanycytic and astrocytic tanycytes have regulatory functions in connection with neurosecretory processes."}
{"id": "PMID:473855", "title": "Seasonal variations in the pharyngeal and pronephric thyroid tissues of the fresh water teleost Puntius sophore (Ham).", "content": "An attempt has been made to study pharyngeal and pronephric thyroid follicles and contribute seasonal changes in them in a fresh water teleost, Puntius sophore. Their activity has been assessed histologically by the study of size of thyroid follicles, the shape and cell height of epithelial cells and the nature of their colloid. These follicles exhibit notable seasonal changes and record two periods of activity, one in summer months from May to August and the other in November.", "contents": "Seasonal variations in the pharyngeal and pronephric thyroid tissues of the fresh water teleost Puntius sophore (Ham). An attempt has been made to study pharyngeal and pronephric thyroid follicles and contribute seasonal changes in them in a fresh water teleost, Puntius sophore. Their activity has been assessed histologically by the study of size of thyroid follicles, the shape and cell height of epithelial cells and the nature of their colloid. These follicles exhibit notable seasonal changes and record two periods of activity, one in summer months from May to August and the other in November."}
{"id": "PMID:473856", "title": "[Occurrence and ultrastructure of mast cells in the hypothalamus of teleost fishes].", "content": "From 56 light-, fluorescence- and electronmicroscopically investigated brains of fishes mast cells could be found only in 4 cases. They were concentrated in the meninges of one pike (Esox licius) and in the meninges and at larger blood-vessels of the hypothalamus of 2 trouts (Salmo trutta fario). In the hypothalamus of a carp (Cyprinus carpio) an infiltration of mast cells was detectable in the meninges, at blood-vessels and capillaries, between neuronal and glial fibres and sub- and intraependymaly. Contrary to the pike the granules of the mast cells of the carp show changes, hinting at release of their content. Junctions between mast cells and capillary walls or ependymal cells are characterized by structures indicating an enhanced cytopempsis. The present results are compared with observations on mast cells in the brain and peritoneum of mammals.", "contents": "[Occurrence and ultrastructure of mast cells in the hypothalamus of teleost fishes]. From 56 light-, fluorescence- and electronmicroscopically investigated brains of fishes mast cells could be found only in 4 cases. They were concentrated in the meninges of one pike (Esox licius) and in the meninges and at larger blood-vessels of the hypothalamus of 2 trouts (Salmo trutta fario). In the hypothalamus of a carp (Cyprinus carpio) an infiltration of mast cells was detectable in the meninges, at blood-vessels and capillaries, between neuronal and glial fibres and sub- and intraependymaly. Contrary to the pike the granules of the mast cells of the carp show changes, hinting at release of their content. Junctions between mast cells and capillary walls or ependymal cells are characterized by structures indicating an enhanced cytopempsis. The present results are compared with observations on mast cells in the brain and peritoneum of mammals."}
{"id": "PMID:473857", "title": "Physiology of circulation in the architecture of Anura.", "content": "A functional relationship exists between the size of erythrocyte and size of capillary passage at metamorphosis. The shift in red cell a/b (major/minor axis) ratio in relation to the changes in cross-sectional capillary area is signficant. These changes have been worked out in thyroxine induced Rana cyanophlyctis tadpoles in comparison with untreated controls. The physiological aspects of phylogenetic origin in relation to ontogenesis of lymphatic sinuses are summed up. A further correlation between the narrowing of capillaries and the sprouting and spreading of lymph sinuses is postulated.", "contents": "Physiology of circulation in the architecture of Anura. A functional relationship exists between the size of erythrocyte and size of capillary passage at metamorphosis. The shift in red cell a/b (major/minor axis) ratio in relation to the changes in cross-sectional capillary area is signficant. These changes have been worked out in thyroxine induced Rana cyanophlyctis tadpoles in comparison with untreated controls. The physiological aspects of phylogenetic origin in relation to ontogenesis of lymphatic sinuses are summed up. A further correlation between the narrowing of capillaries and the sprouting and spreading of lymph sinuses is postulated."}
{"id": "PMID:473858", "title": "Histochemical studies on iron absorption in Ophiocephalus punctatus and Heteropneustes fossilis.", "content": "Iron absorption in both the teleost fishes Ophiocephalus punctatus and Heteropneustes fossilis, initially starts after 2h of feeding the iron diet. However, at this stage, there is no absorption of iron in the posterior intestine of both the fishes. Absorption initially starts along the brush border of enterocytes of the villi. Later, it gets accumulated in the supranuclear region of the epithelial cells and then through these cells, it is transported into the cores of the villi. The absorbed amount ultimately reaches along the bases of the villi. Through the blood capillaries, which are situated in the submucosa, absorbed iron is passed on to the blood stream. Although, the entire intestine of both the fishes is able to absorb iron but the regional variations have been noted. In contrast to the posterior intestine, the anterior and middle intestine of both the fishes show better iron absorption. The pyloric caeca of Ophiocephalus have comparatively less affinity for iron absorption. However, the intestine of Heteropneustes shows more affinity for iron absorption than the intestine of Ophiocephalus.", "contents": "Histochemical studies on iron absorption in Ophiocephalus punctatus and Heteropneustes fossilis. Iron absorption in both the teleost fishes Ophiocephalus punctatus and Heteropneustes fossilis, initially starts after 2h of feeding the iron diet. However, at this stage, there is no absorption of iron in the posterior intestine of both the fishes. Absorption initially starts along the brush border of enterocytes of the villi. Later, it gets accumulated in the supranuclear region of the epithelial cells and then through these cells, it is transported into the cores of the villi. The absorbed amount ultimately reaches along the bases of the villi. Through the blood capillaries, which are situated in the submucosa, absorbed iron is passed on to the blood stream. Although, the entire intestine of both the fishes is able to absorb iron but the regional variations have been noted. In contrast to the posterior intestine, the anterior and middle intestine of both the fishes show better iron absorption. The pyloric caeca of Ophiocephalus have comparatively less affinity for iron absorption. However, the intestine of Heteropneustes shows more affinity for iron absorption than the intestine of Ophiocephalus."}
{"id": "PMID:473859", "title": "Influence of 6-mercaptopurine on the prenatal development of the rat cortex.", "content": "The prenatal cortex development after the application of 6-Mercaptopurine (6-MP) was studied in the rat embryo. 24 h after application of a single dose of 6-MP on day 12, 13, 14 or 15 of gestation first cell lesions occured in the form of necroses. 48 h after injection of the drug these necroses were most pronounced. Their extent and distribution pattern was, however, dependent on the developmental stage. In the early developmental phase, on days 12 and 13, massive necroses could be observed in the intermediate zone. At later stages of development (drug application on day 14 or 15) damaged cells could occasionally also be found scattered over the whole ventricular wall. The late toxic response to 6-MP treatment suggests that, after incorporation of metabolites of the agent into DNA during the S-phase, at least one additional S-phase is completed before the disturbance created in a cell is sufficient to cause necrosis. The necrosis may be a result of an action of 6-MP directly on DNA or via an affect on transcription. The different extent of the lesion during the various developmental phases suggests different growth dynamics of the cortex.", "contents": "Influence of 6-mercaptopurine on the prenatal development of the rat cortex. The prenatal cortex development after the application of 6-Mercaptopurine (6-MP) was studied in the rat embryo. 24 h after application of a single dose of 6-MP on day 12, 13, 14 or 15 of gestation first cell lesions occured in the form of necroses. 48 h after injection of the drug these necroses were most pronounced. Their extent and distribution pattern was, however, dependent on the developmental stage. In the early developmental phase, on days 12 and 13, massive necroses could be observed in the intermediate zone. At later stages of development (drug application on day 14 or 15) damaged cells could occasionally also be found scattered over the whole ventricular wall. The late toxic response to 6-MP treatment suggests that, after incorporation of metabolites of the agent into DNA during the S-phase, at least one additional S-phase is completed before the disturbance created in a cell is sufficient to cause necrosis. The necrosis may be a result of an action of 6-MP directly on DNA or via an affect on transcription. The different extent of the lesion during the various developmental phases suggests different growth dynamics of the cortex."}
{"id": "PMID:473860", "title": "[Cellular ultrastructure of the superior cervical ganglion of the rat in homo- and heterografts following organ culture of the transplant].", "content": "Transplantation of the superior cervical ganglion of rat, after by organe culture retains ultrastructural integrity of the cells for a longer period than transplantation alone. Whatever the graft--homo or heterograft--neuronal survival is extended. Apart from minuts differences, S.I.F. cells show the same evolution. Ultrastructural features modified after culture always return to their initial state. Numerous after the culture always return to their initial state. Numerous after the culture, the lysosomes disappear in the first weeks following the graft. This cytoplasmic epuration is very important, since it might regenerate the cell and dilate its antigenic properties. Without the graft, the cytoplasmic epuration does not take place; vascularization is considered as a favourable factor of evolution. The association graft-culture has a double impact on survival of SCG cells. The culture initiates a cellular epuration, the graft quickens and perfects the return to a normal morphology.", "contents": "[Cellular ultrastructure of the superior cervical ganglion of the rat in homo- and heterografts following organ culture of the transplant]. Transplantation of the superior cervical ganglion of rat, after by organe culture retains ultrastructural integrity of the cells for a longer period than transplantation alone. Whatever the graft--homo or heterograft--neuronal survival is extended. Apart from minuts differences, S.I.F. cells show the same evolution. Ultrastructural features modified after culture always return to their initial state. Numerous after the culture always return to their initial state. Numerous after the culture, the lysosomes disappear in the first weeks following the graft. This cytoplasmic epuration is very important, since it might regenerate the cell and dilate its antigenic properties. Without the graft, the cytoplasmic epuration does not take place; vascularization is considered as a favourable factor of evolution. The association graft-culture has a double impact on survival of SCG cells. The culture initiates a cellular epuration, the graft quickens and perfects the return to a normal morphology."}
{"id": "PMID:473861", "title": "Observations on the Leydig cells in the male East African spring hare (Pedetes surdaster larvalis).", "content": "The morphology of Leydig cells of the testis of sexually mature and sexually immature spring hares was studied. The cytoplasm of the Leydig of cells the sexually immature spring hares was packed with large lipid droplets leaving little space for the other organelles. Smooth endoplasmic reticulum was poorly developed and occasionally formed concentric layers of fenestrated cisterns around the large lipid droplets. The Leydig of cells the sexually mature spring hares were almost devoid of lipid droplets and their cytoplasm was occupied by abundant tubular smooth endoplasmic reticulum. Cells which shared characteristics with both immature Leydig cells and undifferentiated mesenchymal cells were observed in the limiting membrane of the seminiferous tubulus. These Leydig-like cells may play a role in the differentiation of Leydig cells in the spring hare.", "contents": "Observations on the Leydig cells in the male East African spring hare (Pedetes surdaster larvalis). The morphology of Leydig cells of the testis of sexually mature and sexually immature spring hares was studied. The cytoplasm of the Leydig of cells the sexually immature spring hares was packed with large lipid droplets leaving little space for the other organelles. Smooth endoplasmic reticulum was poorly developed and occasionally formed concentric layers of fenestrated cisterns around the large lipid droplets. The Leydig of cells the sexually mature spring hares were almost devoid of lipid droplets and their cytoplasm was occupied by abundant tubular smooth endoplasmic reticulum. Cells which shared characteristics with both immature Leydig cells and undifferentiated mesenchymal cells were observed in the limiting membrane of the seminiferous tubulus. These Leydig-like cells may play a role in the differentiation of Leydig cells in the spring hare."}
{"id": "PMID:473862", "title": "Seasonal changes in the corpuscles of Stannius and the gonads of the catfish, Heteropneustes fossilis (Bloch).", "content": "The seasonal changes in the corpuscles of Stannius (CS) and the gonads of the catfish, Heteropneustes fossilis were studied. The annual sex cycle of the fish has been divided into 4 phases on the basis of the variations in the gonosomatic index and histocytological features displayed by the testes and ovaries. There is a rise in the percentage of aldehyde fuchsin (AF)-positive cells in the CS and an increase in the nuclear diameter, at the beginning of preparatory period (February). In the prespawning period (May--June) the AF-positive cells undergo degranulation. A slight regranulation and rise in the percentage of AF-positive cells occurs during early spawing period (July). During the postspawning phase (September--January) the corpuscles remain predominated by AF-negative cells and show histolytical changes; the nuclear indices are reduced. In view of the concomitant changes occurring in the CS and the gonads the possibility of some direct or indirect relationship between the two has been discussed, although it is difficult to ascertain whether the changes in the corpuscles are cause or consequence of the gonadal cycle.", "contents": "Seasonal changes in the corpuscles of Stannius and the gonads of the catfish, Heteropneustes fossilis (Bloch). The seasonal changes in the corpuscles of Stannius (CS) and the gonads of the catfish, Heteropneustes fossilis were studied. The annual sex cycle of the fish has been divided into 4 phases on the basis of the variations in the gonosomatic index and histocytological features displayed by the testes and ovaries. There is a rise in the percentage of aldehyde fuchsin (AF)-positive cells in the CS and an increase in the nuclear diameter, at the beginning of preparatory period (February). In the prespawning period (May--June) the AF-positive cells undergo degranulation. A slight regranulation and rise in the percentage of AF-positive cells occurs during early spawing period (July). During the postspawning phase (September--January) the corpuscles remain predominated by AF-negative cells and show histolytical changes; the nuclear indices are reduced. In view of the concomitant changes occurring in the CS and the gonads the possibility of some direct or indirect relationship between the two has been discussed, although it is difficult to ascertain whether the changes in the corpuscles are cause or consequence of the gonadal cycle."}
{"id": "PMID:473864", "title": "[Indications for the reconstruction of the female breast].", "content": "In 7 years reconstruction of breasts amputated due to carcinoma have been carried out 139 times in our clinic, with good aesthetic results. This operation was mostly performed on female patients with ages ranging between 30--50 years. A survey is made of the state of malignancy of the tumour of our patients in which the breast amputation was carried out and of the histological findings. A discussion will be made concerning at which point of time reconstruction should take place depending upon the state of malignancy of the tumour at the time of the first operation. Reoccurrances of primary tumours were found in 15 patients, 4 tumours of the contra-lateral breasts, and 3 micro-metastases in the scar tissue following amputation were discovered during reconstruction. The evidence gathered by us during the time of the survey confirmed the significance that breast reconstruction following amputation has upon the total personality of the patient.", "contents": "[Indications for the reconstruction of the female breast]. In 7 years reconstruction of breasts amputated due to carcinoma have been carried out 139 times in our clinic, with good aesthetic results. This operation was mostly performed on female patients with ages ranging between 30--50 years. A survey is made of the state of malignancy of the tumour of our patients in which the breast amputation was carried out and of the histological findings. A discussion will be made concerning at which point of time reconstruction should take place depending upon the state of malignancy of the tumour at the time of the first operation. Reoccurrances of primary tumours were found in 15 patients, 4 tumours of the contra-lateral breasts, and 3 micro-metastases in the scar tissue following amputation were discovered during reconstruction. The evidence gathered by us during the time of the survey confirmed the significance that breast reconstruction following amputation has upon the total personality of the patient."}
{"id": "PMID:473865", "title": "[Structural analysis of the capsules around breast prostheses (light and electron microscopic studies)].", "content": "Fibrous tissue capsules around silicone-gel and saline filled breast implants were examined by light microscopy as well as by transmission and scanning electron microscopy. This study revealed the following details: 1. Regularly arranged dense connective tissue forms the basis of the reactively developed capsules around the breast implants. This tissue contains bundles of collagenous fibers which are densely packed and lie parallel to each other forming structures of great tensile strengthes. 2. The outer surface of the connective tissue capsules contain reticulum fibers, the diameter of which is small. The fibers branch variously to form a netlike framework, which obviously can support the mechanical significance of the collagenous material. 3. At the inner surface fibrocytes and histiocytes are single layered and form an epithelium-like structure. These cells possess specializations of their free surface as microvilli, microplicae, and cell protrusions. In some areas dense connective tissue forms a felt-like surface. 4. Contractile fibroblasts (myofibroblasts) were found in these fibrous capsules. These cells share electron microscopic features of both smooth muscle cells and fibroblasts. Along with the rough endoplasmic reticulum and Golgi apparatus, these cells have long thin bundles of microfilaments. 5. The connective tissue of the capsules contains also capillaries and fibrocytes, which are usually deployed along bundles of collagen fibers and appear in sections as fusiform elements with long processes. The fibrocytes also contain lysosomes, and because these are involved in hydrolysis or digestion of material within the cell, their appearance depends upon their functional state, leading to their characteristic pleomorphism. 6. The frequently resulting unshapelyness of the breast implants cannot be caused by the action of the so-called myofibroblasts, the number of which is too small, but more likely because of the unelestic arrangement of large amounts of collagenous material.", "contents": "[Structural analysis of the capsules around breast prostheses (light and electron microscopic studies)]. Fibrous tissue capsules around silicone-gel and saline filled breast implants were examined by light microscopy as well as by transmission and scanning electron microscopy. This study revealed the following details: 1. Regularly arranged dense connective tissue forms the basis of the reactively developed capsules around the breast implants. This tissue contains bundles of collagenous fibers which are densely packed and lie parallel to each other forming structures of great tensile strengthes. 2. The outer surface of the connective tissue capsules contain reticulum fibers, the diameter of which is small. The fibers branch variously to form a netlike framework, which obviously can support the mechanical significance of the collagenous material. 3. At the inner surface fibrocytes and histiocytes are single layered and form an epithelium-like structure. These cells possess specializations of their free surface as microvilli, microplicae, and cell protrusions. In some areas dense connective tissue forms a felt-like surface. 4. Contractile fibroblasts (myofibroblasts) were found in these fibrous capsules. These cells share electron microscopic features of both smooth muscle cells and fibroblasts. Along with the rough endoplasmic reticulum and Golgi apparatus, these cells have long thin bundles of microfilaments. 5. The connective tissue of the capsules contains also capillaries and fibrocytes, which are usually deployed along bundles of collagen fibers and appear in sections as fusiform elements with long processes. The fibrocytes also contain lysosomes, and because these are involved in hydrolysis or digestion of material within the cell, their appearance depends upon their functional state, leading to their characteristic pleomorphism. 6. The frequently resulting unshapelyness of the breast implants cannot be caused by the action of the so-called myofibroblasts, the number of which is too small, but more likely because of the unelestic arrangement of large amounts of collagenous material."}
{"id": "PMID:473866", "title": "[Andromastectomy].", "content": "Andromastectomy, that is to say the removal of the male mammary glands, should prevent the development of Gynaecomastia which is both painful and deforming and may occur subsequent to certain illnesses or hormone treatment.", "contents": "[Andromastectomy]. Andromastectomy, that is to say the removal of the male mammary glands, should prevent the development of Gynaecomastia which is both painful and deforming and may occur subsequent to certain illnesses or hormone treatment."}
{"id": "PMID:473867", "title": "[Lengthening of the 1. metacarpal according to Matev after loss of the thumb].", "content": "In cases where loss of the thumb has occurred, the gripping function of the hand can be pariallly restored by the process of extending the first metacarpel. In our opinion the method according to Matev, involving distraction and the interpositioning of a bony wedge is a relatively simple operation and compared with similar methods has the advantage of achieving positive results in regard to the length. The only prerequisite for this method is good skin and soft tissue condition in the area of the stump.", "contents": "[Lengthening of the 1. metacarpal according to Matev after loss of the thumb]. In cases where loss of the thumb has occurred, the gripping function of the hand can be pariallly restored by the process of extending the first metacarpel. In our opinion the method according to Matev, involving distraction and the interpositioning of a bony wedge is a relatively simple operation and compared with similar methods has the advantage of achieving positive results in regard to the length. The only prerequisite for this method is good skin and soft tissue condition in the area of the stump."}
{"id": "PMID:473899", "title": "[Need-information organization of brain activity].", "content": "Experimental results and literature data point to the leading role of four brain structures in the genesis of emotional states and in the organization of goal directed behaviour. Owing to the functioning of the frontal parts of the neocortex behaviour is oriented to signals with a high probability of their reinforcement, and owing to the hypothalamus--to satisfaction of the dominant need. Unlike the neocortex the hippocampus is necessary for reactions to signals of lowly probable events, which is typical of emotionally exicted brain. The amygdala complx participates in creation of a balance, a dynamic co-existence between completing needs (motivations) and corresponding emotions. As a result, behaviour becomes more plastic and adequate to the given situation.", "contents": "[Need-information organization of brain activity]. Experimental results and literature data point to the leading role of four brain structures in the genesis of emotional states and in the organization of goal directed behaviour. Owing to the functioning of the frontal parts of the neocortex behaviour is oriented to signals with a high probability of their reinforcement, and owing to the hypothalamus--to satisfaction of the dominant need. Unlike the neocortex the hippocampus is necessary for reactions to signals of lowly probable events, which is typical of emotionally exicted brain. The amygdala complx participates in creation of a balance, a dynamic co-existence between completing needs (motivations) and corresponding emotions. As a result, behaviour becomes more plastic and adequate to the given situation."}
{"id": "PMID:473905", "title": "[Stimulus recognition as a factor in establishing the effective properties of the signal].", "content": "An attempt was made to estimate the importance of preliminary perceptual learning of the recognition of indifferent stimulus for the formation of effective properties of the trigger signal of conditioned reflex functional system. The dynamics of the elaboration of instrumental conditioned reflexes was compared: 1) when an unfamiliar signal was introduced into the experimental environment (control experiments). 2) in case of introduction of a perceptually familiar signal, 3) after \"erasing\" of acquired perceptual experience by electroshock. The experiments revealed that a preliminary perceptual learning contributes to the formation of the effective properties of the trigger signal.", "contents": "[Stimulus recognition as a factor in establishing the effective properties of the signal]. An attempt was made to estimate the importance of preliminary perceptual learning of the recognition of indifferent stimulus for the formation of effective properties of the trigger signal of conditioned reflex functional system. The dynamics of the elaboration of instrumental conditioned reflexes was compared: 1) when an unfamiliar signal was introduced into the experimental environment (control experiments). 2) in case of introduction of a perceptually familiar signal, 3) after \"erasing\" of acquired perceptual experience by electroshock. The experiments revealed that a preliminary perceptual learning contributes to the formation of the effective properties of the trigger signal."}
{"id": "PMID:473904", "title": "[Effect of low frequency stimulation of the head of the caudate nucleus on an instrumental defense reaction in dogs].", "content": "The effect of low-frequency stimulation of the caudate nucleus head (CNH) on motor components of instrumental defensive reaction (IDR) (IDR \"coordination\" program) and on realization of the instrumental task itself--avoidance of electrical current (IDR \"sense\" program) was compared in chronic experiments on six dogs with a model of IDR. Low-frequency stimulation of CNH of the right and left hemispheres exerted an inhibitory influence on the IDR components which characterize its coordination program. Inhibitory effects increased proportionally to the intensity of stimulation current. The changes were mostly pronounced in the early electromyographic components of the support extremity's muscle activity preceding IDR, which suggests an important role of CNH in rearrangment of the posture, preceding voluntary movement. Achievement of the instrumental task itself (avoidance of the electrical current) was inhibited only with a very high intensity of stimulation (higher than 1 ma).", "contents": "[Effect of low frequency stimulation of the head of the caudate nucleus on an instrumental defense reaction in dogs]. The effect of low-frequency stimulation of the caudate nucleus head (CNH) on motor components of instrumental defensive reaction (IDR) (IDR \"coordination\" program) and on realization of the instrumental task itself--avoidance of electrical current (IDR \"sense\" program) was compared in chronic experiments on six dogs with a model of IDR. Low-frequency stimulation of CNH of the right and left hemispheres exerted an inhibitory influence on the IDR components which characterize its coordination program. Inhibitory effects increased proportionally to the intensity of stimulation current. The changes were mostly pronounced in the early electromyographic components of the support extremity's muscle activity preceding IDR, which suggests an important role of CNH in rearrangment of the posture, preceding voluntary movement. Achievement of the instrumental task itself (avoidance of the electrical current) was inhibited only with a very high intensity of stimulation (higher than 1 ma)."}
{"id": "PMID:473907", "title": "[EEG correlation and cortical neurons spike activity during elaboration of a defensive conditioned reflex].", "content": "Elaboration of electro-defensive conditioned reflex to light in rabbits has shown, that the values of summary cross-correlation coefficients between EEG and impulse activity of cortical neurones do not change significantly as compared to the control. In these conditions does not change either the part of the neurones that have a significant correlation of their impulse discharges with any configuration of the EEG wave. During 6--10 days of conditioning there occurs a decrease in the amplitudes of both positive and negative EEG oscillations, connected with unit discharges in the neocortex. An EEG quantum has being recorded, i. e. a depolarization potential, directly connected with the generation of spikes by the studied nervous elements.", "contents": "[EEG correlation and cortical neurons spike activity during elaboration of a defensive conditioned reflex]. Elaboration of electro-defensive conditioned reflex to light in rabbits has shown, that the values of summary cross-correlation coefficients between EEG and impulse activity of cortical neurones do not change significantly as compared to the control. In these conditions does not change either the part of the neurones that have a significant correlation of their impulse discharges with any configuration of the EEG wave. During 6--10 days of conditioning there occurs a decrease in the amplitudes of both positive and negative EEG oscillations, connected with unit discharges in the neocortex. An EEG quantum has being recorded, i. e. a depolarization potential, directly connected with the generation of spikes by the studied nervous elements."}
{"id": "PMID:473901", "title": "[Dynamics of the formation and interrelationship of trace phenomena in 6--7-year-old children].", "content": "The dynamics of formation, the reproduction characteristics and interrelation of trace phenomena in conditions of a stable vs. unstable pause between the stimuli in Konorski test were studied in 148 6--7 year old children. It has been shown that with a stable interstimuli pause, along with correct voluntary reactions, based on stimuli traces, two types of electropgraphic trace reaction to time are elaborated--anticipatory and post-stimulatory. In this case trace phenomena are formed much more rapidly and their connection with the volume of the children's short-term memory is tighter than in experiments with an unstable interstimuli pause. It has been also shown that children's school resluts significantly correlate with the caracteristics of their mnemic activity--the volume of the short-term memory and Konorski test data.", "contents": "[Dynamics of the formation and interrelationship of trace phenomena in 6--7-year-old children]. The dynamics of formation, the reproduction characteristics and interrelation of trace phenomena in conditions of a stable vs. unstable pause between the stimuli in Konorski test were studied in 148 6--7 year old children. It has been shown that with a stable interstimuli pause, along with correct voluntary reactions, based on stimuli traces, two types of electropgraphic trace reaction to time are elaborated--anticipatory and post-stimulatory. In this case trace phenomena are formed much more rapidly and their connection with the volume of the children's short-term memory is tighter than in experiments with an unstable interstimuli pause. It has been also shown that children's school resluts significantly correlate with the caracteristics of their mnemic activity--the volume of the short-term memory and Konorski test data."}
{"id": "PMID:473906", "title": "[Effect of unilateral section of the X septo-hippocampal pathways on the electrical activity of the dorsal hippocampus and behavior of rabbits].", "content": "The influence of unilateral section of the septo-hippocampal pathway on the electrical activity of the dorsal hippocampus of both hemispheres was studied in conditions of elaborated behaviour. The analysis of EEG data of the fields CA1 and CA3 before the section revealed an interconnection of the theta-rhythm and behaviour. Ablation of the septal input caused in the ipsilateral hippocampus a sharp decrease in the activity amplitude and in the number of theta-waves as well as an increase in beta-frequencies and an impairment of interconnection between the frequency bands of the theta-rhythm and the forms of behavioural act. Meanwhile, in the contralateral hippocampus, despite a certain decrease in the activity amplitude and an increase in the number of rapid oscillations, no sharp decrease in theta-waves was visible and the correlation of the theta-rhythm with the behaviour forms was clearly pronounced.", "contents": "[Effect of unilateral section of the X septo-hippocampal pathways on the electrical activity of the dorsal hippocampus and behavior of rabbits]. The influence of unilateral section of the septo-hippocampal pathway on the electrical activity of the dorsal hippocampus of both hemispheres was studied in conditions of elaborated behaviour. The analysis of EEG data of the fields CA1 and CA3 before the section revealed an interconnection of the theta-rhythm and behaviour. Ablation of the septal input caused in the ipsilateral hippocampus a sharp decrease in the activity amplitude and in the number of theta-waves as well as an increase in beta-frequencies and an impairment of interconnection between the frequency bands of the theta-rhythm and the forms of behavioural act. Meanwhile, in the contralateral hippocampus, despite a certain decrease in the activity amplitude and an increase in the number of rapid oscillations, no sharp decrease in theta-waves was visible and the correlation of the theta-rhythm with the behaviour forms was clearly pronounced."}
{"id": "PMID:473908", "title": "[Responses of visual cortex neurons to photic and acoustic stimuli in rabbits reared in the dark].", "content": "Unit responses in the visual cortex to photic and acoustic stimuli were studied in intact and visually deprived rabbits raised in the dark since birth up to 2.5--5 months of age. Photic flashes, tones, single and rhythmical clicks were used as stimuli. A significant increase of the number of bimodal units was revealed in the cortex of visually deprived rabbits. The level of significance of the statistical estimate of the change in the number of phonoreactive units (its increase due to deprivation) amounts to 92%. The nature of responses to flashes and the ratio of photoreactive units does not change due to deprivation, which suggests their genetic determination. Possible mechanisms of the re-distribution of neuronal reactivity in the rabbit visual cortex in conditions of deprivation are discussed.", "contents": "[Responses of visual cortex neurons to photic and acoustic stimuli in rabbits reared in the dark]. Unit responses in the visual cortex to photic and acoustic stimuli were studied in intact and visually deprived rabbits raised in the dark since birth up to 2.5--5 months of age. Photic flashes, tones, single and rhythmical clicks were used as stimuli. A significant increase of the number of bimodal units was revealed in the cortex of visually deprived rabbits. The level of significance of the statistical estimate of the change in the number of phonoreactive units (its increase due to deprivation) amounts to 92%. The nature of responses to flashes and the ratio of photoreactive units does not change due to deprivation, which suggests their genetic determination. Possible mechanisms of the re-distribution of neuronal reactivity in the rabbit visual cortex in conditions of deprivation are discussed."}
{"id": "PMID:473909", "title": "[Changes in cortical neuron input resistance and the stimulation threshold of its electroexcitable membrane by a depolarizing current during the habituation process].", "content": "The habituation of neurones in the turtle visual cortex is accompanied by changes in their input resistance and in the threshold of stimulation of their electro-excitable membrane by depolarizing current. Input resistance of the neurone during monotoneous stimulation decreases throughout habituation and increases following an alien stimulus. The threshold becomes higher if the current acts as a monotoneous stimulus and gets lower if it acts as an alien stimulus. Input resistance of a habituated neurone and its threshold are restored after alien stimulation and spontaneously--after interruption. These changes are specific to the stimulation, appear during its action, and are reversible. They form postsynaptic mechanisms of habituation.", "contents": "[Changes in cortical neuron input resistance and the stimulation threshold of its electroexcitable membrane by a depolarizing current during the habituation process]. The habituation of neurones in the turtle visual cortex is accompanied by changes in their input resistance and in the threshold of stimulation of their electro-excitable membrane by depolarizing current. Input resistance of the neurone during monotoneous stimulation decreases throughout habituation and increases following an alien stimulus. The threshold becomes higher if the current acts as a monotoneous stimulus and gets lower if it acts as an alien stimulus. Input resistance of a habituated neurone and its threshold are restored after alien stimulation and spontaneously--after interruption. These changes are specific to the stimulation, appear during its action, and are reversible. They form postsynaptic mechanisms of habituation."}
{"id": "PMID:473925", "title": "Toxic trace metals in food. I. A new voltammetric procedure for toxic trace metal control of wines.", "content": "A new highly sensitive and particularly reliable analytical procedure for the precise determination of the most relevant toxic trace metals cadmium, lead and copper in wine is presented. It consists of a simple and convenient sample pretreatment by UV irradiation (1,5 h, 500-W-Hg-lamp) to release the toxic trace metals bound by organic substances and subsequent voltammetry (DPASV) of cadmium and lead simultaneously at a mercury film electrode (MFE) formed in situ on a vitreous carbon carrier and of copper on a gold electrode. Particular emphasis has been placed on the efficient exclusion of interference due to contamination. Only special wines, with more than 10% of sugar, require substitution of UV irradiation by a wet digestion also described. The procedure may be easily expanded to include the determination of mercury and zinc.", "contents": "Toxic trace metals in food. I. A new voltammetric procedure for toxic trace metal control of wines. A new highly sensitive and particularly reliable analytical procedure for the precise determination of the most relevant toxic trace metals cadmium, lead and copper in wine is presented. It consists of a simple and convenient sample pretreatment by UV irradiation (1,5 h, 500-W-Hg-lamp) to release the toxic trace metals bound by organic substances and subsequent voltammetry (DPASV) of cadmium and lead simultaneously at a mercury film electrode (MFE) formed in situ on a vitreous carbon carrier and of copper on a gold electrode. Particular emphasis has been placed on the efficient exclusion of interference due to contamination. Only special wines, with more than 10% of sugar, require substitution of UV irradiation by a wet digestion also described. The procedure may be easily expanded to include the determination of mercury and zinc."}
{"id": "PMID:473928", "title": "Transformation of the capillary wall elements in synovial tissue in rheumatoid arthritis.", "content": "1. The morphologically comprehensible starting point of rheumatoid arthritis is a permeability change of the synovial capillaries. 2. In the context of rheumatoid arthritis all three building elements of the blood capillaries undergo a transformation. 3. In the endothelial cells and the pericytes the endoplasmatic reticulum, the Golgi-apparatus, as well as the mitochondria can be multiplied (progressive transformation). The cell structures can, however, be subject to a degeneration (regressive transformation). The basement membrane as a secretion product, in contrast, undergoes exclusively quantitative changes. 4. The transformation possibilities of the capillary elements will be treated and classified on a graph under general pathological aspects. 5. As an element of the connective tissue the endothelial cell, as are lining cell and stroma cell in rheumatoid arthritis, is subject to transforming influences which, however, at the synovial capillary will be the starter of fibrin exudation into the joint cavity.", "contents": "Transformation of the capillary wall elements in synovial tissue in rheumatoid arthritis. 1. The morphologically comprehensible starting point of rheumatoid arthritis is a permeability change of the synovial capillaries. 2. In the context of rheumatoid arthritis all three building elements of the blood capillaries undergo a transformation. 3. In the endothelial cells and the pericytes the endoplasmatic reticulum, the Golgi-apparatus, as well as the mitochondria can be multiplied (progressive transformation). The cell structures can, however, be subject to a degeneration (regressive transformation). The basement membrane as a secretion product, in contrast, undergoes exclusively quantitative changes. 4. The transformation possibilities of the capillary elements will be treated and classified on a graph under general pathological aspects. 5. As an element of the connective tissue the endothelial cell, as are lining cell and stroma cell in rheumatoid arthritis, is subject to transforming influences which, however, at the synovial capillary will be the starter of fibrin exudation into the joint cavity."}
{"id": "PMID:473927", "title": "[The value of morphology in the diagnosis of chronic polyarthritis].", "content": "The clinician's question concerning the value of morphological findings in diagnosis of rheumatoid arthritis is answered by the pathologist. After a short discussion of clinical problems and the ARA-criteria, at first the diagnostic importance of synovial cytology in RA is dealt with. The patient's history, biopsy technique and methods are the most important prerequisites of an optimal assistance of the pathologist to a definite diagnosis; they are reviewed in short paragraphs. These data are the bases of the morphological evaluation which is discussed in detail. This is the most important part of the answer to questions of the clinician; the pathologist is not only able to verify the clinical diagnosis of rheumatoid synovitis or of RA but often he may decisively judge the course and activity of the disease and the therapeutic success.", "contents": "[The value of morphology in the diagnosis of chronic polyarthritis]. The clinician's question concerning the value of morphological findings in diagnosis of rheumatoid arthritis is answered by the pathologist. After a short discussion of clinical problems and the ARA-criteria, at first the diagnostic importance of synovial cytology in RA is dealt with. The patient's history, biopsy technique and methods are the most important prerequisites of an optimal assistance of the pathologist to a definite diagnosis; they are reviewed in short paragraphs. These data are the bases of the morphological evaluation which is discussed in detail. This is the most important part of the answer to questions of the clinician; the pathologist is not only able to verify the clinical diagnosis of rheumatoid synovitis or of RA but often he may decisively judge the course and activity of the disease and the therapeutic success."}
{"id": "PMID:473929", "title": "[The therapeutic effect of glycosaminoglycan polysulfate (Arteparon) in arthroses depending on the mode of administration (intraarticular or intramuscular)].", "content": "The effect of GAGPS (Arteparon) was investigated on patients with osteoarthritis of hip-joints in dependence from the method of application. By that way 50 patients got each 6 injections with 125 mg/0,5 ml Arteparon intraarticular and 50 patients each 10 injections with 125 mg/0,5 ml Arteparon intramuscular. We have documented the qualities of pain, function and motility of the treated hip-joints. The tests applied have demonstrated that there is no significant difference between the both applications, in both groups the effect was good to the same extent. The therapeutic effect and the further indications by intramuscular administration of the GAGPS are discussed.", "contents": "[The therapeutic effect of glycosaminoglycan polysulfate (Arteparon) in arthroses depending on the mode of administration (intraarticular or intramuscular)]. The effect of GAGPS (Arteparon) was investigated on patients with osteoarthritis of hip-joints in dependence from the method of application. By that way 50 patients got each 6 injections with 125 mg/0,5 ml Arteparon intraarticular and 50 patients each 10 injections with 125 mg/0,5 ml Arteparon intramuscular. We have documented the qualities of pain, function and motility of the treated hip-joints. The tests applied have demonstrated that there is no significant difference between the both applications, in both groups the effect was good to the same extent. The therapeutic effect and the further indications by intramuscular administration of the GAGPS are discussed."}
{"id": "PMID:473931", "title": "[Autoradiographic studies on the mitotic and productive metabolism of the articular cartilage in mouse genetic arthritis].", "content": "With suitable radioactively labelled precursors the mitotic (using 3H-thymidine) and the productive metabolisms (using 3H-proline and 35S-sulphate) were investigated on various kinds of joint- etc. cartilage from mice with genetically caused arthrosis. The autoradiographic analyses were performed using 3H-thymidine-, 3H-proline- and 35S-sulphate-indices and the statistical evaluation of the findings led to the following results: the synthesis level of sulphated glycosaminoglycans (GAG) in the cartilage ground substance is lower for 5--6 month-old mice than for the 16--17 month-old animals of this genetic arthrosis strain. This is most distinct in the 3rd (= basal) zone of the knee-joint cartilage which increased to double the comparable value in this period. In all 3 zones of the knee-joint cartilage GAG-synthesis is considerably greater than the collagen synthesis according to these autoradiographic analyses. These findings can be detected before any histologically or histochemically demonstrable cartilage alterations and represent therefore the earliest signs of a so-called \"pre-arthrosis\". Collagen synthesis decreases thereby, and the same holds for DNA-synthesis and the mitotic metabolism of the cartilage cells - according to the findings to date. However the question is still under investigation whether arthrosis (like atherosclerosis) also starts with an increase in cell-turnover. This question is not only of theoretical interest, it has practical clinical consequences, even for the treatment. These investigations also serve this clinically relevant question because arthrosis is the most common human joint disease.", "contents": "[Autoradiographic studies on the mitotic and productive metabolism of the articular cartilage in mouse genetic arthritis]. With suitable radioactively labelled precursors the mitotic (using 3H-thymidine) and the productive metabolisms (using 3H-proline and 35S-sulphate) were investigated on various kinds of joint- etc. cartilage from mice with genetically caused arthrosis. The autoradiographic analyses were performed using 3H-thymidine-, 3H-proline- and 35S-sulphate-indices and the statistical evaluation of the findings led to the following results: the synthesis level of sulphated glycosaminoglycans (GAG) in the cartilage ground substance is lower for 5--6 month-old mice than for the 16--17 month-old animals of this genetic arthrosis strain. This is most distinct in the 3rd (= basal) zone of the knee-joint cartilage which increased to double the comparable value in this period. In all 3 zones of the knee-joint cartilage GAG-synthesis is considerably greater than the collagen synthesis according to these autoradiographic analyses. These findings can be detected before any histologically or histochemically demonstrable cartilage alterations and represent therefore the earliest signs of a so-called \"pre-arthrosis\". Collagen synthesis decreases thereby, and the same holds for DNA-synthesis and the mitotic metabolism of the cartilage cells - according to the findings to date. However the question is still under investigation whether arthrosis (like atherosclerosis) also starts with an increase in cell-turnover. This question is not only of theoretical interest, it has practical clinical consequences, even for the treatment. These investigations also serve this clinically relevant question because arthrosis is the most common human joint disease."}
{"id": "PMID:473954", "title": "[Liver biopsy findings after intake of oral contraceptives (author's transl)].", "content": "115 liver biopsies of women who had taken oral contraceptives have been examined. In 106 of these case (68.4%) morphological modifications were found which may be considered as effects of the hormonal contraceptives. Dilations of the sinusoids with differently proliferated Kupffer cells were predominantly evident (73 cases). Medicamentally induced hepatitis (20 cases) and cholestatic hepatosis (5 cases) were considerably rare. The sinusoid ectasias may be understood as a consequence of liver damage by steroid hormones. The pathogenesis of the changes is unknown.", "contents": "[Liver biopsy findings after intake of oral contraceptives (author's transl)]. 115 liver biopsies of women who had taken oral contraceptives have been examined. In 106 of these case (68.4%) morphological modifications were found which may be considered as effects of the hormonal contraceptives. Dilations of the sinusoids with differently proliferated Kupffer cells were predominantly evident (73 cases). Medicamentally induced hepatitis (20 cases) and cholestatic hepatosis (5 cases) were considerably rare. The sinusoid ectasias may be understood as a consequence of liver damage by steroid hormones. The pathogenesis of the changes is unknown."}
{"id": "PMID:473955", "title": "[Sebaceous lymphadenoma of the parotid gland (author's transl)].", "content": "A case of sebaceous lymphadenoma close to the parotid gland--the twelfth in the literature--is presented. The morphologic findings and histogenetic considerations are reported together with a short review of literature.", "contents": "[Sebaceous lymphadenoma of the parotid gland (author's transl)]. A case of sebaceous lymphadenoma close to the parotid gland--the twelfth in the literature--is presented. The morphologic findings and histogenetic considerations are reported together with a short review of literature."}
{"id": "PMID:473956", "title": "Nitrogen fixers in the rhizosphere of certain desert plants.", "content": "Six species of common desert range plants; Achillea sp., Aristida plumosa, Artemisia herba-alba, Haloxylon articulatum, and Heliotropium ramosissimum were colleted from Western Desert in Iraq. Counts of Azotobacter spp. and total bacteria were estimated in both rhizosphere and non-rhizosphere soils. Biomass and total nitrogen content of the plants were determined. The R/N ratio (ratio between rhizosphere to non-rhizosphere) for Azotobacter sp. ranged between 1 to 19.5 with an average of 4.96. The number of Azotobacter in the rhizosphere ranged between 6.8 X 10(3) to 47 X 10(3) cells/g, while in the non-rhizosphere it ranged from 2 X 10(3) to 21 X 10(3). Aristida plumosa harboured the highest number, i.e., 47 X 10(3) cells/g, while Artemisia herba-alba showed the least, i.e., 6.8 X 10(3) cells/g. Total counts of bacteria in the rhizosphere ranged between 17.2 X 10(6) and 97 X 10(6) cells/g, that of the non-rhizosphere between 1.5 X 10(6) and 18 X 10(6) cells/g. The R/N ratio ranged between 2.03 X 22.5 with an average of 7.28. An appreciable gain of dry weight and total nitrogen was observed. Between 13 and 152 g with an average of 78.9 g dry weight and between 347 and 6684.0 mg with an average of 2452.0 mg nitrogen per plant were found. The possible contribution of Azotobacter to the nitrogen economy of the plants is discussed and further suggested.", "contents": "Nitrogen fixers in the rhizosphere of certain desert plants. Six species of common desert range plants; Achillea sp., Aristida plumosa, Artemisia herba-alba, Haloxylon articulatum, and Heliotropium ramosissimum were colleted from Western Desert in Iraq. Counts of Azotobacter spp. and total bacteria were estimated in both rhizosphere and non-rhizosphere soils. Biomass and total nitrogen content of the plants were determined. The R/N ratio (ratio between rhizosphere to non-rhizosphere) for Azotobacter sp. ranged between 1 to 19.5 with an average of 4.96. The number of Azotobacter in the rhizosphere ranged between 6.8 X 10(3) to 47 X 10(3) cells/g, while in the non-rhizosphere it ranged from 2 X 10(3) to 21 X 10(3). Aristida plumosa harboured the highest number, i.e., 47 X 10(3) cells/g, while Artemisia herba-alba showed the least, i.e., 6.8 X 10(3) cells/g. Total counts of bacteria in the rhizosphere ranged between 17.2 X 10(6) and 97 X 10(6) cells/g, that of the non-rhizosphere between 1.5 X 10(6) and 18 X 10(6) cells/g. The R/N ratio ranged between 2.03 X 22.5 with an average of 7.28. An appreciable gain of dry weight and total nitrogen was observed. Between 13 and 152 g with an average of 78.9 g dry weight and between 347 and 6684.0 mg with an average of 2452.0 mg nitrogen per plant were found. The possible contribution of Azotobacter to the nitrogen economy of the plants is discussed and further suggested."}
{"id": "PMID:473957", "title": "Response of irrigated groundnuts (Arachis hypogaea L.) to urea fertilization in the central rainlands of the Sudan.", "content": "The response of 3 varieties of groundnuts, i.e.: Ashford (A), Barberton (B), and Corinte (C), to different doses of urea nitrogen under irrigation was studied for three years. As a result of extensive tissue analysis, the critical needs of Barberton and Corinte for nitrogen were found to be 1500 ppm NO3-N and 3.7% total-N. Variety Ashford, which outyielded both, did not respond to urea nitrogen. A linear correlation coefficient of r = 0.85 for pod and straw yields gave a pod to straw ratio of 0.72. Kernel protein and oil content were not affected by nitrogen. Larger nodule counts and their total-N indicated better yields.", "contents": "Response of irrigated groundnuts (Arachis hypogaea L.) to urea fertilization in the central rainlands of the Sudan. The response of 3 varieties of groundnuts, i.e.: Ashford (A), Barberton (B), and Corinte (C), to different doses of urea nitrogen under irrigation was studied for three years. As a result of extensive tissue analysis, the critical needs of Barberton and Corinte for nitrogen were found to be 1500 ppm NO3-N and 3.7% total-N. Variety Ashford, which outyielded both, did not respond to urea nitrogen. A linear correlation coefficient of r = 0.85 for pod and straw yields gave a pod to straw ratio of 0.72. Kernel protein and oil content were not affected by nitrogen. Larger nodule counts and their total-N indicated better yields."}
{"id": "PMID:473958", "title": "[Model trials for isolation of soft rot bacteria by media containing pectic substances (author's transl)].", "content": "1. Under aerobic conditions bacterial soft rots are caused in the most cases by pectolytic Erwinia, Pseudomonas, and Bacillus spp. 2. Using 7 soft rotting strains several selective media were tested for their ability to sure a quick and exact isolation and differentiation of these pathogens. By bile salt-lactose-medium all Erwinia spp. under test could be isolated. For isolation of pectinolytic Pseudomonas strains the D4 medium of Kado and Heskett was suitable as the best one. 3. By the use of substrats containing pectic substances the results of isolation could be essentially improved: So, a sure differentiation of soft rotting bacteria and saprophytic organisms already upon the substrate and in addition, the isolation of pectinolytic Bacillus strains also became possible. The pectinolytic activity of the test strains on pectin-double layer-media was dependent upon the composition of the basal medium. 4. On the base of the results we obtained an isolation scheme is proposed, that allowed to indicate the pectolytic active bacilli (after heating to 80 degrees C for 10 min) on thioglycollate medium covered by a pectin layer, the Erwinia spp. on Stewart-Medium covered by pectin layer and the pectolytic pseudomonads on FPA-medium described by Sands, Hankin and Zucker (containing citrus pectin) to the equal time. 5. The trials also demonstrated, that the preparing of double layer-media instead of sodium polypectate mostly being recommended in literature lower estered pectic compounds can be used successfully.", "contents": "[Model trials for isolation of soft rot bacteria by media containing pectic substances (author's transl)]. 1. Under aerobic conditions bacterial soft rots are caused in the most cases by pectolytic Erwinia, Pseudomonas, and Bacillus spp. 2. Using 7 soft rotting strains several selective media were tested for their ability to sure a quick and exact isolation and differentiation of these pathogens. By bile salt-lactose-medium all Erwinia spp. under test could be isolated. For isolation of pectinolytic Pseudomonas strains the D4 medium of Kado and Heskett was suitable as the best one. 3. By the use of substrats containing pectic substances the results of isolation could be essentially improved: So, a sure differentiation of soft rotting bacteria and saprophytic organisms already upon the substrate and in addition, the isolation of pectinolytic Bacillus strains also became possible. The pectinolytic activity of the test strains on pectin-double layer-media was dependent upon the composition of the basal medium. 4. On the base of the results we obtained an isolation scheme is proposed, that allowed to indicate the pectolytic active bacilli (after heating to 80 degrees C for 10 min) on thioglycollate medium covered by a pectin layer, the Erwinia spp. on Stewart-Medium covered by pectin layer and the pectolytic pseudomonads on FPA-medium described by Sands, Hankin and Zucker (containing citrus pectin) to the equal time. 5. The trials also demonstrated, that the preparing of double layer-media instead of sodium polypectate mostly being recommended in literature lower estered pectic compounds can be used successfully."}
{"id": "PMID:473959", "title": "Effect of some pesticides on certain micro-organisms, contributing to soil fertility.", "content": "A pot experiment was run for studying the effect of Temik (insecticide and nematocide), Orthocide (fungicide), and Treflan (herbicide) on certain desirable soil micro-organisms. Total counts of bacteria, actinomycetes or fungi were mostly lower in the treated than in the untreated soil. Aerobic cellulose-decomposers were either stimulated or depressed. Despite the temporarily slight stimulation, observed in some instances at the initial periods, the effect of the pesticides on counts of Azotobacter, N-fixing clostridia, and ammonifiers was afterwards depressed. The period of retardation or stimulation differed according to type of pesticide and type of micro-organisms under study. However, the autotrophic nitrifying bacteria, especially ammonium-oxidizers, seemed to be more sensitive and their counts in treated soil were sharply depressed.", "contents": "Effect of some pesticides on certain micro-organisms, contributing to soil fertility. A pot experiment was run for studying the effect of Temik (insecticide and nematocide), Orthocide (fungicide), and Treflan (herbicide) on certain desirable soil micro-organisms. Total counts of bacteria, actinomycetes or fungi were mostly lower in the treated than in the untreated soil. Aerobic cellulose-decomposers were either stimulated or depressed. Despite the temporarily slight stimulation, observed in some instances at the initial periods, the effect of the pesticides on counts of Azotobacter, N-fixing clostridia, and ammonifiers was afterwards depressed. The period of retardation or stimulation differed according to type of pesticide and type of micro-organisms under study. However, the autotrophic nitrifying bacteria, especially ammonium-oxidizers, seemed to be more sensitive and their counts in treated soil were sharply depressed."}
{"id": "PMID:473960", "title": "[Dioxohexahydrotriazine, a new synthetic antiphytoviral compound (author's transl)].", "content": "This paper reports the first results of studies made to determine the antiviral activity of dioxo hexahydro triazine, a newly developed antiphytoviral compound. After treatment with dioxo hexahydro triazine, the concentration of helical potato virus X in leaves of Nicotiana tabacum \"Samsun\" was found to be considerably reduced in both the serological test and local lesion test conducted on Nicotiana glutinosa at temperatures of 14 degrees C +/- 3 degrees C. In secondarily infected leaves, the reduction of the concentration of potato virus X in the range of from 1 mol/l to 10(-3) mol/l was so that it was no longer possible for viruses to be detected serologically. In vitro, however, dioxo hexahydro triazine, which was added to the inoculum, did not produce a significant change in the activity of the virion of potato virus X. Therefore, it is reasonable to assume that dioxo hexahydro triazine tends to affect replication steps of viruses. The use of dioxo hexahydro triazine also had the effect of greatly and highly significantly reducing the concentration of the isodiametric cucumber mosaic virus.", "contents": "[Dioxohexahydrotriazine, a new synthetic antiphytoviral compound (author's transl)]. This paper reports the first results of studies made to determine the antiviral activity of dioxo hexahydro triazine, a newly developed antiphytoviral compound. After treatment with dioxo hexahydro triazine, the concentration of helical potato virus X in leaves of Nicotiana tabacum \"Samsun\" was found to be considerably reduced in both the serological test and local lesion test conducted on Nicotiana glutinosa at temperatures of 14 degrees C +/- 3 degrees C. In secondarily infected leaves, the reduction of the concentration of potato virus X in the range of from 1 mol/l to 10(-3) mol/l was so that it was no longer possible for viruses to be detected serologically. In vitro, however, dioxo hexahydro triazine, which was added to the inoculum, did not produce a significant change in the activity of the virion of potato virus X. Therefore, it is reasonable to assume that dioxo hexahydro triazine tends to affect replication steps of viruses. The use of dioxo hexahydro triazine also had the effect of greatly and highly significantly reducing the concentration of the isodiametric cucumber mosaic virus."}
{"id": "PMID:473961", "title": "Distribution of extracellular proteolytic activities among various yeast.", "content": "A general screening test for extracellular proteolytic activities was carried out on 24 cultures belonging to 14 genera of yeasts. The screening was performed using gelatine and casein as sole nitrogen sources under two sets of pH conditions. Potent proteolytic yeast cultures belonging to both Ascomycetes and Deuteromycetes were detected, particularly certain members of the genera Endomycopsis, Metschnikowia, Debaromyces, Rhodotorula, and Candida spec. Most detected proteolytic cultures were active on both casein and gelatine substrates, possibly suggesting one enzyme system in each case with wide substrate specificity. Physiological studies revealed that maximum proteolysis occurred after 3--4 days of aerobic incubation and that some strains were able to hydrolyze egg albumin.", "contents": "Distribution of extracellular proteolytic activities among various yeast. A general screening test for extracellular proteolytic activities was carried out on 24 cultures belonging to 14 genera of yeasts. The screening was performed using gelatine and casein as sole nitrogen sources under two sets of pH conditions. Potent proteolytic yeast cultures belonging to both Ascomycetes and Deuteromycetes were detected, particularly certain members of the genera Endomycopsis, Metschnikowia, Debaromyces, Rhodotorula, and Candida spec. Most detected proteolytic cultures were active on both casein and gelatine substrates, possibly suggesting one enzyme system in each case with wide substrate specificity. Physiological studies revealed that maximum proteolysis occurred after 3--4 days of aerobic incubation and that some strains were able to hydrolyze egg albumin."}
{"id": "PMID:473963", "title": "Chemical control of nitrification under rice in the central rainlands of the Sudan.", "content": "2-amino-4-chloro-6-methyl pyrimidine \"AM\" was used as a selective inhibitor of nitrifying organisms to prevent denitrification losses. Zero, 6, and 12 kg/ha of the chemical, 43 and 86 kg/ha urea-N or their equivalents were used in vitro and in vivo experiments. In the in vitro experiment, clear and significant inhibition lasted for 6 weeks. In both the pot and field experiments inhibition lasted shorter and only insignificant increases in yield and yield components were attained. The value of this chemical for rice under the soil and climatic conditions of the Central Rainlands seems to be small, though it seems promising for lighter (leaching) soils.", "contents": "Chemical control of nitrification under rice in the central rainlands of the Sudan. 2-amino-4-chloro-6-methyl pyrimidine \"AM\" was used as a selective inhibitor of nitrifying organisms to prevent denitrification losses. Zero, 6, and 12 kg/ha of the chemical, 43 and 86 kg/ha urea-N or their equivalents were used in vitro and in vivo experiments. In the in vitro experiment, clear and significant inhibition lasted for 6 weeks. In both the pot and field experiments inhibition lasted shorter and only insignificant increases in yield and yield components were attained. The value of this chemical for rice under the soil and climatic conditions of the Central Rainlands seems to be small, though it seems promising for lighter (leaching) soils."}
{"id": "PMID:473964", "title": "Associative symbiosis of Azotobacter chroococcum and higher plants.", "content": "The association between a selected strain of Azotobacter chroococcum and seven plants was investigated in water cultures under sterile conditions. Azotobacter population progressively increased in the nutrient solution and on the rhizoplane. Microbial propagation depends on the type of plant, being much higher in presence of wheat, followed by barley, maize, broad bean, and cotton, while in presence of fenugreek and lentil lower rates of multiplication were recorded. Inoculation increased the dry weight of plants by 5--12% and in length by 3--18% in addition to increased nitrogen content of plants and nutrient solution. Nitrogen balance showed no significant change in systems devoid of Azotobacter, but association between plants and the microorganism invariably showed positive results. The extent of N2-fixation depends on the type of plants; higher gains were recorded in presence of non-leguminous plants.", "contents": "Associative symbiosis of Azotobacter chroococcum and higher plants. The association between a selected strain of Azotobacter chroococcum and seven plants was investigated in water cultures under sterile conditions. Azotobacter population progressively increased in the nutrient solution and on the rhizoplane. Microbial propagation depends on the type of plant, being much higher in presence of wheat, followed by barley, maize, broad bean, and cotton, while in presence of fenugreek and lentil lower rates of multiplication were recorded. Inoculation increased the dry weight of plants by 5--12% and in length by 3--18% in addition to increased nitrogen content of plants and nutrient solution. Nitrogen balance showed no significant change in systems devoid of Azotobacter, but association between plants and the microorganism invariably showed positive results. The extent of N2-fixation depends on the type of plants; higher gains were recorded in presence of non-leguminous plants."}
{"id": "PMID:473965", "title": "Effect of Azotobacter inoculation on plant growth and soil nitrogen.", "content": "The validity of seed bacterization with Azotobacter chroococcum in soils of variable densities of naturally present azotobacters was studied. Inoculation of barley grains had no effect on counts of total microflora, neither in rhizosphere nor in root-free soil, but significantly increased Azotobacter population, especially in the rhizosphere. The rate of colonization in the root region was much higher when soil initially harboured low Azotobacter densities. Bacterization improved plant growth and increased soil nitrogen through nitrogen fixation. Nitrogen balance in soils showed higher gains in the inoculated treatments over the uninoculated analogues of 30--98 ppm.", "contents": "Effect of Azotobacter inoculation on plant growth and soil nitrogen. The validity of seed bacterization with Azotobacter chroococcum in soils of variable densities of naturally present azotobacters was studied. Inoculation of barley grains had no effect on counts of total microflora, neither in rhizosphere nor in root-free soil, but significantly increased Azotobacter population, especially in the rhizosphere. The rate of colonization in the root region was much higher when soil initially harboured low Azotobacter densities. Bacterization improved plant growth and increased soil nitrogen through nitrogen fixation. Nitrogen balance in soils showed higher gains in the inoculated treatments over the uninoculated analogues of 30--98 ppm."}
{"id": "PMID:473966", "title": "The effect of temperature and age of strains of Fusarium oxysporum on its enzymatic activity.", "content": "The activities of pectin-methyl-esterase, polygalacturonase, cellulase, amylase, saccharase, and protease of strains of Fusarium oxysporum (Schlecht.) f.sp. pisi (Linford) were studied. The selected strains showed different symptoms and different degrees of pathogenicity on the host plant. The measurements were performed during the growth of strains at constant temperatures and, in another experiment, on the day at different temperatures at which the individual strains were grown. Activities were determined by the plate methods and by the spectrophotometric method. It has been found that enzyme activity of the strains with different degree of pathogenicity show considerable differences in dependence on temperature and growth dynamics.", "contents": "The effect of temperature and age of strains of Fusarium oxysporum on its enzymatic activity. The activities of pectin-methyl-esterase, polygalacturonase, cellulase, amylase, saccharase, and protease of strains of Fusarium oxysporum (Schlecht.) f.sp. pisi (Linford) were studied. The selected strains showed different symptoms and different degrees of pathogenicity on the host plant. The measurements were performed during the growth of strains at constant temperatures and, in another experiment, on the day at different temperatures at which the individual strains were grown. Activities were determined by the plate methods and by the spectrophotometric method. It has been found that enzyme activity of the strains with different degree of pathogenicity show considerable differences in dependence on temperature and growth dynamics."}
{"id": "PMID:473967", "title": "Different manifestations of the pathogenity of some strains of Fusarium oxysporum f. sp. pisi.", "content": "Different cultivation and morphology characteristics were found in 10 monospore isolates of Fusarium oxysporum f. sp. pisi, obtained from yellowing and wilting plants of pea (Pisum sativum L.). The isolates of the fungus were obtained from distant geographical regions of Czechoslovakia and from various cultivars and hybrids of pea. After inoculation of roots, followed by constant conditions of incubation of the Meteor and Jupiter cultivars having their origin at the Plant-breeding Station at Luzany u Prestic, the isolates caused various symptoms of disease, each isolate showed a different degree of pathogenity. The variability of the pathogenity of the isolates depended on the host. Its manifestation, in turn, depended on the dynamics of growth and development of the pathogen as well as the host. The following symptoms could be observed during the pathogenesis: the dying of cotyledons after the contact of the main root with the inoculum, the dying of young plants (the plants usually forming two stems), wilting of young plants, yellowing of bottom leaves and wilting beginning from the bottom leaves, stunted growth, and plant deformation. The symptoms of disease are related to the changes in vascular system.", "contents": "Different manifestations of the pathogenity of some strains of Fusarium oxysporum f. sp. pisi. Different cultivation and morphology characteristics were found in 10 monospore isolates of Fusarium oxysporum f. sp. pisi, obtained from yellowing and wilting plants of pea (Pisum sativum L.). The isolates of the fungus were obtained from distant geographical regions of Czechoslovakia and from various cultivars and hybrids of pea. After inoculation of roots, followed by constant conditions of incubation of the Meteor and Jupiter cultivars having their origin at the Plant-breeding Station at Luzany u Prestic, the isolates caused various symptoms of disease, each isolate showed a different degree of pathogenity. The variability of the pathogenity of the isolates depended on the host. Its manifestation, in turn, depended on the dynamics of growth and development of the pathogen as well as the host. The following symptoms could be observed during the pathogenesis: the dying of cotyledons after the contact of the main root with the inoculum, the dying of young plants (the plants usually forming two stems), wilting of young plants, yellowing of bottom leaves and wilting beginning from the bottom leaves, stunted growth, and plant deformation. The symptoms of disease are related to the changes in vascular system."}
{"id": "PMID:473968", "title": "Electron microscopic cytochemistry of polysaccharides in bacteria Xanthomonas fuscans, the cause of the bean fuscous blight.", "content": "High resolution cytochemical staining of polysaccharides in the cells of the bean fuscous blight bacteria Xanthomonas fuscans (Burkholder) Bur. revealed extensive deposits of a periodic acid-oxidizable compound in the cytoplasm, which accumulated at the onset of polysaccharide excretion and occupied a substantial part of the cell volume. In the course of subsequent culture growth the intracellular polysaccharides gradually disappeared and polysaccharide microfibrils were visualized in the medium. Our observations indicate that the exuded phytopathogenic polysaccharides may be synthetized in the cytoplasm of the bacteria.", "contents": "Electron microscopic cytochemistry of polysaccharides in bacteria Xanthomonas fuscans, the cause of the bean fuscous blight. High resolution cytochemical staining of polysaccharides in the cells of the bean fuscous blight bacteria Xanthomonas fuscans (Burkholder) Bur. revealed extensive deposits of a periodic acid-oxidizable compound in the cytoplasm, which accumulated at the onset of polysaccharide excretion and occupied a substantial part of the cell volume. In the course of subsequent culture growth the intracellular polysaccharides gradually disappeared and polysaccharide microfibrils were visualized in the medium. Our observations indicate that the exuded phytopathogenic polysaccharides may be synthetized in the cytoplasm of the bacteria."}
{"id": "PMID:473970", "title": "[Surgical management of reno-vascular hypertony (author's transl)].", "content": "Only in 2/3 out of a total of 33 patients suffering from renal artery stenosis surgery was indicated. Thrombendarterectomy and saphenous patch are the method of choice for reconstructive procedures. In 87% of successful reconstructions the functional result was satisfactory. The follow-up gave evidence of change to the worse due to the progress of the underlying disease.", "contents": "[Surgical management of reno-vascular hypertony (author's transl)]. Only in 2/3 out of a total of 33 patients suffering from renal artery stenosis surgery was indicated. Thrombendarterectomy and saphenous patch are the method of choice for reconstructive procedures. In 87% of successful reconstructions the functional result was satisfactory. The follow-up gave evidence of change to the worse due to the progress of the underlying disease."}
{"id": "PMID:473971", "title": "[Planimetric measurement of kidney size in patients with stenosis of the renal artery (author's transl)].", "content": "Comparison of pre- and postoperative angiograms reveals no significant statistical difference concerning kidney size after successful reconstruction of the renal artery. This examination is based on planimetric data of 10 patients with haemodynamical important stenoses of the renal artery (including one patient with bilateral stenosis).", "contents": "[Planimetric measurement of kidney size in patients with stenosis of the renal artery (author's transl)]. Comparison of pre- and postoperative angiograms reveals no significant statistical difference concerning kidney size after successful reconstruction of the renal artery. This examination is based on planimetric data of 10 patients with haemodynamical important stenoses of the renal artery (including one patient with bilateral stenosis)."}
{"id": "PMID:473981", "title": "[Brain lipids of a mammoth, Elephas primigenius, which died more than 40,000 years ago].", "content": "Studies have been made on the brain lipids of the 6--7-month mammoth which remained in the eternal ice for more than 40.000 years. Thin layer chromatography of chloroformmethanol extract of the brain lipids shows that all glycerophospholipids in the brain were destroyed. On the contrary, sphingophospholipid sphingomyelin yielded the evident spot which was identified by specific reactions and by comparison with sphingomyelin from the brain of rat. Sphingomyelin content was evaluated. Using gas-liquid chromatography, fatty acid composition of sphingomyelin was investigated. It was found to be close to that in contemporary mammals. Other shingolipids -- cerebrosides, sulfatides, gangliosides -- persisted (probably, only partially) and were studied quantitatively. Relative content of cerebrosides with normal fatty acids and hydrooxyacids was determined. Studies were also made on fatty acid composition of cerebrosides, sulfatides and gangliosides, as well as on the composition of spingosine bases of gangliosides. Free cholesterol was found in the brain of the mammoth. Other sterols were not detected. With respect to quantitative evaluation of the preserved lipids, it should be mentioned that on the one hand, the brain underwent dehydration which increased lipid content per a unit of \"wet\" weight, whereas on the other one lipids were partially degraded, this process decreasing their content.", "contents": "[Brain lipids of a mammoth, Elephas primigenius, which died more than 40,000 years ago]. Studies have been made on the brain lipids of the 6--7-month mammoth which remained in the eternal ice for more than 40.000 years. Thin layer chromatography of chloroformmethanol extract of the brain lipids shows that all glycerophospholipids in the brain were destroyed. On the contrary, sphingophospholipid sphingomyelin yielded the evident spot which was identified by specific reactions and by comparison with sphingomyelin from the brain of rat. Sphingomyelin content was evaluated. Using gas-liquid chromatography, fatty acid composition of sphingomyelin was investigated. It was found to be close to that in contemporary mammals. Other shingolipids -- cerebrosides, sulfatides, gangliosides -- persisted (probably, only partially) and were studied quantitatively. Relative content of cerebrosides with normal fatty acids and hydrooxyacids was determined. Studies were also made on fatty acid composition of cerebrosides, sulfatides and gangliosides, as well as on the composition of spingosine bases of gangliosides. Free cholesterol was found in the brain of the mammoth. Other sterols were not detected. With respect to quantitative evaluation of the preserved lipids, it should be mentioned that on the one hand, the brain underwent dehydration which increased lipid content per a unit of \"wet\" weight, whereas on the other one lipids were partially degraded, this process decreasing their content."}
{"id": "PMID:473984", "title": "[Effect of nembutal anesthesia, electric stimulation of the paws and elaboration of a defensive reflex on acetylcholinesterase activity and protein content in different regions of rat brain].", "content": "Pentobarbitone sodium anaesthesia was found to produce an increase in protein content in some regions of the rat brain, i. e. posterior cortex, caudate nucleus, and a decrease in protein content in the ventral cortex. Acetylcholinesterase expressed in terms of wet weight was found to increase in the cerebellum, medulla, and to decrease in the medial cortex, hippocampus, thalamus and caudate nucleus. The changes in activity were not explicable in terms of a direct effect of the anaesthetic on the enzyme. A decrease in protein content of rat brain was observed in the frontal cortex, ventral cortex, hippocampus and caudate nucleus after electrical shocks. Following shock avoidance conditioning procedure (shuttle-box), decreases in protein content were observed in the medial cortex, posterior cortex, cerebellum and ventral cortex; in the thalamus an increase in protein content was observed. Changes in AChE activity were observed following footshock in the frontal cortex and medulla where there was an increase in activity and in the caudate nucleus, hypothalamus, thalamus, and olfactory tubercule where there was a decrease in activity. Following shock avoidance conditioning the activity of the AChE increased in posterior cortex, hippocampus, thalamus and hypothalamus and the activity of the enzyme decreased in the ventral cortex.", "contents": "[Effect of nembutal anesthesia, electric stimulation of the paws and elaboration of a defensive reflex on acetylcholinesterase activity and protein content in different regions of rat brain]. Pentobarbitone sodium anaesthesia was found to produce an increase in protein content in some regions of the rat brain, i. e. posterior cortex, caudate nucleus, and a decrease in protein content in the ventral cortex. Acetylcholinesterase expressed in terms of wet weight was found to increase in the cerebellum, medulla, and to decrease in the medial cortex, hippocampus, thalamus and caudate nucleus. The changes in activity were not explicable in terms of a direct effect of the anaesthetic on the enzyme. A decrease in protein content of rat brain was observed in the frontal cortex, ventral cortex, hippocampus and caudate nucleus after electrical shocks. Following shock avoidance conditioning procedure (shuttle-box), decreases in protein content were observed in the medial cortex, posterior cortex, cerebellum and ventral cortex; in the thalamus an increase in protein content was observed. Changes in AChE activity were observed following footshock in the frontal cortex and medulla where there was an increase in activity and in the caudate nucleus, hypothalamus, thalamus, and olfactory tubercule where there was a decrease in activity. Following shock avoidance conditioning the activity of the AChE increased in posterior cortex, hippocampus, thalamus and hypothalamus and the activity of the enzyme decreased in the ventral cortex."}
{"id": "PMID:473985", "title": "[Sensitivity of Nereis virens polychaetes to mechanical oscillations in water].", "content": "Food and escape reactions of Nereis virens to oscillations of a ball 2 mm in diameter in water have been studied. These antagonistic reactions differ significantly by the range of ball oscillations which evoked them. The range from 10 to 30 Hz appeared to be optimal for food reaction, and the range above 40 Hz -- for escape reaction. Worms are able to detect a ball from a distance of 12--13 mm at optimal oscillation frequency 30 Hz. Possible receptor mechanisms providing these types of reaction are discussed.", "contents": "[Sensitivity of Nereis virens polychaetes to mechanical oscillations in water]. Food and escape reactions of Nereis virens to oscillations of a ball 2 mm in diameter in water have been studied. These antagonistic reactions differ significantly by the range of ball oscillations which evoked them. The range from 10 to 30 Hz appeared to be optimal for food reaction, and the range above 40 Hz -- for escape reaction. Worms are able to detect a ball from a distance of 12--13 mm at optimal oscillation frequency 30 Hz. Possible receptor mechanisms providing these types of reaction are discussed."}
{"id": "PMID:473987", "title": "[Ionic composition of mollusk cells--evolutionary and ecologic aspects].", "content": "Marine molluscs exhibit relative constancy in intracellular potassium at the back ground of significant changes in intracellular sodium during acclimation to differen salinities. These changes, which were observed in cells of the adductor muscle and hepa topancreas, result mainly from active extrusion of sodium (possibly, of chloride as well) from the cell at low salinities and accumulation of these ions within the cell during the increase in salinity. Changes in intracellular concentration of sodium and chloride ions serve presumably as one of the main mechanisms of volume stabilization of cells, which together with the amino acid regulation alleviates the hydration of cells at different salinities. The capacity of cells to keep their potassium accounts for the maintenance of Ki/Ko ratio during changes in cellular volume induced by osmotic effects. These data are discussed in relation to two alternative hypotheses of the decreased and close to the present salinity of ocean at the initial stages of formation of the ionic composition of cells.", "contents": "[Ionic composition of mollusk cells--evolutionary and ecologic aspects]. Marine molluscs exhibit relative constancy in intracellular potassium at the back ground of significant changes in intracellular sodium during acclimation to differen salinities. These changes, which were observed in cells of the adductor muscle and hepa topancreas, result mainly from active extrusion of sodium (possibly, of chloride as well) from the cell at low salinities and accumulation of these ions within the cell during the increase in salinity. Changes in intracellular concentration of sodium and chloride ions serve presumably as one of the main mechanisms of volume stabilization of cells, which together with the amino acid regulation alleviates the hydration of cells at different salinities. The capacity of cells to keep their potassium accounts for the maintenance of Ki/Ko ratio during changes in cellular volume induced by osmotic effects. These data are discussed in relation to two alternative hypotheses of the decreased and close to the present salinity of ocean at the initial stages of formation of the ionic composition of cells."}
{"id": "PMID:473988", "title": "[Cholesterol in the brain of cartilaginous and bony fish].", "content": "Data are presented on the content of cholesterol in the brain of 23 fish species with different phylogenetic position and ecology. Stable and lower cholesterol content (mean level 10.6 mg/g wet tissue) in the brain of 11 species of Chondrichtyes was shown together with variable and higher (mean level 13 mg/g wet tissue) one in the brain of 12 species of Teleostei. The data obtained are discussed in relation to phylogenetic position of the species investigated and ecological factors (depth and temperature). The ratio of cholesterol to phospholipids in the brain of fishes is lower than that in mammals.", "contents": "[Cholesterol in the brain of cartilaginous and bony fish]. Data are presented on the content of cholesterol in the brain of 23 fish species with different phylogenetic position and ecology. Stable and lower cholesterol content (mean level 10.6 mg/g wet tissue) in the brain of 11 species of Chondrichtyes was shown together with variable and higher (mean level 13 mg/g wet tissue) one in the brain of 12 species of Teleostei. The data obtained are discussed in relation to phylogenetic position of the species investigated and ecological factors (depth and temperature). The ratio of cholesterol to phospholipids in the brain of fishes is lower than that in mammals."}
{"id": "PMID:473986", "title": "[Participation of insulin in regulating the metabolism of marine bivalve mollusks].", "content": "Marine bivalve molluscs, the mussel Mytilus galloprovincialis aud the scallop Chlamys (Flexopecten) glaber ponticus, have been injected (intramuscularly or in the haemolymph) with glucose, mammalian insulin, insulin (or insulin-like substance) from molluscs, and anti-insulin serum, checking changes in glucose and fatty acid content of the haemolymph as well as in the content of glycogen and the activity of glycogen synthetase in muscles. After glucose injections, studies were also made on the level of IRI in the haemolymph. Comparison of the data obtained in the present work with those reported earlier for freshwater bivalve molluscs suggests that: 1) metabolic shifts induced by anti-insulin serum are more rapid in vigorous scallops than in sedentary mussels; 2) molluscan insulin (or insulin-like substance) exerts the same effect on metabolic parameters of the molluscs, as mammalian insulin exerts in vertebrates.", "contents": "[Participation of insulin in regulating the metabolism of marine bivalve mollusks]. Marine bivalve molluscs, the mussel Mytilus galloprovincialis aud the scallop Chlamys (Flexopecten) glaber ponticus, have been injected (intramuscularly or in the haemolymph) with glucose, mammalian insulin, insulin (or insulin-like substance) from molluscs, and anti-insulin serum, checking changes in glucose and fatty acid content of the haemolymph as well as in the content of glycogen and the activity of glycogen synthetase in muscles. After glucose injections, studies were also made on the level of IRI in the haemolymph. Comparison of the data obtained in the present work with those reported earlier for freshwater bivalve molluscs suggests that: 1) metabolic shifts induced by anti-insulin serum are more rapid in vigorous scallops than in sedentary mussels; 2) molluscan insulin (or insulin-like substance) exerts the same effect on metabolic parameters of the molluscs, as mammalian insulin exerts in vertebrates."}
{"id": "PMID:473991", "title": "[Visual projections into the telencephalon and diencephalon of the teleost fish Serranus scriba (electrophysiologic study)].", "content": "In acute experiments on Serranus scriba, two discrete foci of visual activity in the telencephalon were shown--in the dorsal pallium and in the central telencephalic area. The latency of focal potentials evoked by optic tectum stimulation was at least 2 msec shorter than that of the responses elicited by optic nerve stimulation. In the region of nucleus rotundus and some other thalamo-pretectal structures, focal potentials and single unit reactions to both types of stimulation were registered. Responses to optic tectum stimulation in nucleus pretectalis exhibited the shortest latency. It is suggested that visual impulses to the central telencephalic area are relayed consequently in the optic tectum and probably in more than one thalamo-pretectal nucleus.", "contents": "[Visual projections into the telencephalon and diencephalon of the teleost fish Serranus scriba (electrophysiologic study)]. In acute experiments on Serranus scriba, two discrete foci of visual activity in the telencephalon were shown--in the dorsal pallium and in the central telencephalic area. The latency of focal potentials evoked by optic tectum stimulation was at least 2 msec shorter than that of the responses elicited by optic nerve stimulation. In the region of nucleus rotundus and some other thalamo-pretectal structures, focal potentials and single unit reactions to both types of stimulation were registered. Responses to optic tectum stimulation in nucleus pretectalis exhibited the shortest latency. It is suggested that visual impulses to the central telencephalic area are relayed consequently in the optic tectum and probably in more than one thalamo-pretectal nucleus."}
{"id": "PMID:473993", "title": "[Morphofunctional characteristics of the optic nerve of the turtle Testudo graeca].", "content": "Electron microscopic investigation indicates that in T. graeca the optic nerve consists of about 216,000 fibers of which 11% are myelinated and 89%--unmyelinated ones. The diameter of myelinated fibers varies within 0.2-2.5 mu, the main bulk of fibers (42%) having the diameter 0.5-0.7 mu. The diameter of unmyelinated fibers varies between 0.1 and 0.35 mu. With respect to their diameter, 8% of the fibers of both groups overlap each other. During electrical stimulation of the optic nerve, action potential in the latter is recorded which consists of two high amplitude components. The first component (propagation velocity 1.5 m/sec) presumably corresponds to conduction of excitation along myelinated fibers, the second one (propagation velocity 0.6 m/sec)--along unmyelinated fibers.", "contents": "[Morphofunctional characteristics of the optic nerve of the turtle Testudo graeca]. Electron microscopic investigation indicates that in T. graeca the optic nerve consists of about 216,000 fibers of which 11% are myelinated and 89%--unmyelinated ones. The diameter of myelinated fibers varies within 0.2-2.5 mu, the main bulk of fibers (42%) having the diameter 0.5-0.7 mu. The diameter of unmyelinated fibers varies between 0.1 and 0.35 mu. With respect to their diameter, 8% of the fibers of both groups overlap each other. During electrical stimulation of the optic nerve, action potential in the latter is recorded which consists of two high amplitude components. The first component (propagation velocity 1.5 m/sec) presumably corresponds to conduction of excitation along myelinated fibers, the second one (propagation velocity 0.6 m/sec)--along unmyelinated fibers."}
{"id": "PMID:473989", "title": "[Fatty acids of the cerebrosides (1-beta-glycosylceramides) and sulfatides (sulfoglycosylceramides) of the brain of the river lamprey Lampetra fluviatilis].", "content": "The analysis of the fatty acids of cerebrosides and sulfatides from the brain of the lamprey L. fluviatilis has been carried out. Only normal fatty acids were revealed by TLC of total methyl esters of the fatty acid of cerebrosides and sulfatides. The hydroxy fatty acids are apparently absent. The composition of the normal fatty acids of cerebrosides and sulfatides is very similar. TLC revealed fatty acids containing from 14 to 27 C mainly saturated and small amount of monoenes. The fatty acid pattern of cerebrosides and sulfatides from lamprey brain resembles that from the brain of other vertebrates. The peculiarity of the fatty acid composition of these glycolipids in the lamprey brain is the high proportion of short chain (C14--C18) saturated acids, especially of palmitic acid. The fatty acid composition of cerebrosides and sulfatides from lamprey brain which does not contain myelin is rather similar to that of mammalian brain in early development before the onset of myelination.", "contents": "[Fatty acids of the cerebrosides (1-beta-glycosylceramides) and sulfatides (sulfoglycosylceramides) of the brain of the river lamprey Lampetra fluviatilis]. The analysis of the fatty acids of cerebrosides and sulfatides from the brain of the lamprey L. fluviatilis has been carried out. Only normal fatty acids were revealed by TLC of total methyl esters of the fatty acid of cerebrosides and sulfatides. The hydroxy fatty acids are apparently absent. The composition of the normal fatty acids of cerebrosides and sulfatides is very similar. TLC revealed fatty acids containing from 14 to 27 C mainly saturated and small amount of monoenes. The fatty acid pattern of cerebrosides and sulfatides from lamprey brain resembles that from the brain of other vertebrates. The peculiarity of the fatty acid composition of these glycolipids in the lamprey brain is the high proportion of short chain (C14--C18) saturated acids, especially of palmitic acid. The fatty acid composition of cerebrosides and sulfatides from lamprey brain which does not contain myelin is rather similar to that of mammalian brain in early development before the onset of myelination."}
{"id": "PMID:473995", "title": "[Functional organization of the rabbit hypothalamo-paleocortical system].", "content": "Investigation of the ascending hypothalamic connections with principal paleocortical centers (the olfactory tubercule, prepiryform cortex) indicates that the most short latent, poorly exhausted by rhythmic stimulation or by neuronal reactions EPs in these olfactory centers are elicited by stimulation of phylogenetically ancient hypothalamic areas of the anterior LPO and lateral LHA. It was also shown that the third deep layer of the prepiryform cortex is the main center for total ascending afferentation from these ancient parts of the hypothalamus. EPs and cellular responses to stimulation of phylogenetically new hypothalamic regions could be revealed with difficulties and were characterized by long latent periods or high degree of exhaustion by rhythmic stimulation. Paleo-hypothalamic system in the rabbit exhibits strict organization of functional connections. It was demonstrated that during stimulation of the main olfactory centers of the forebrain, EPs and neuronal responses originate chiefly in the anterior (LPO) and in the narrow zone of the lateral hypothalamus. Under the conditions used, cellular responses in VMH and SMA were not revealed.", "contents": "[Functional organization of the rabbit hypothalamo-paleocortical system]. Investigation of the ascending hypothalamic connections with principal paleocortical centers (the olfactory tubercule, prepiryform cortex) indicates that the most short latent, poorly exhausted by rhythmic stimulation or by neuronal reactions EPs in these olfactory centers are elicited by stimulation of phylogenetically ancient hypothalamic areas of the anterior LPO and lateral LHA. It was also shown that the third deep layer of the prepiryform cortex is the main center for total ascending afferentation from these ancient parts of the hypothalamus. EPs and cellular responses to stimulation of phylogenetically new hypothalamic regions could be revealed with difficulties and were characterized by long latent periods or high degree of exhaustion by rhythmic stimulation. Paleo-hypothalamic system in the rabbit exhibits strict organization of functional connections. It was demonstrated that during stimulation of the main olfactory centers of the forebrain, EPs and neuronal responses originate chiefly in the anterior (LPO) and in the narrow zone of the lateral hypothalamus. Under the conditions used, cellular responses in VMH and SMA were not revealed."}
{"id": "PMID:474001", "title": "[Experimental exacerbation of latent Q rickettsial infection].", "content": "The evidence of the prolonged persistence of Coxiella burnetii in experimental animals was obtained. Cyclophosphamide was shown to be capable of activating Q rickettsiosis in guinea pigs, and the period of the aggravation of this infection was found to be accompanied by a decrease in IgG concentration and a relative increase in IgM content.", "contents": "[Experimental exacerbation of latent Q rickettsial infection]. The evidence of the prolonged persistence of Coxiella burnetii in experimental animals was obtained. Cyclophosphamide was shown to be capable of activating Q rickettsiosis in guinea pigs, and the period of the aggravation of this infection was found to be accompanied by a decrease in IgG concentration and a relative increase in IgM content."}
{"id": "PMID:473990", "title": "[Composition and structure of the chief gangliosides in the brain of the lamprey Lampetra fluviatilis].", "content": "It has been shown that 4-sphingenine is the main sphingoid in lamprey brain gangliosides. Saturated and monoenoic fatty acids were found to predominate, the main fatty acids in the lamprey brain are presented by stearic (43-49% of total fatty acids) and oleic ones. N-acetylneuraminic acid is the only sialic acid found in gangliosides from the lamprey brain. Other components of ganglioside molecules are glucose, galactose and N-acetylgalactosamine. Two main lamprey brain gangliosides which constitute more than 90% of lipid-bound sialic acid, were found to be trisialogangliosides. It was shown that both gangliosides have the following structure in their molecules: (formula see text). They differ in the position of two other sialic acid residues. Data on lamprey brain gangliosides in the literature are practically absent. The data obtained in the present study confirmed the conclusions on changes in the hydrophobic part of ganglioside molecule in evolution of vertebrates and made it possible to define more exactly the molecular evolution of ganglioside carbohydrate component.", "contents": "[Composition and structure of the chief gangliosides in the brain of the lamprey Lampetra fluviatilis]. It has been shown that 4-sphingenine is the main sphingoid in lamprey brain gangliosides. Saturated and monoenoic fatty acids were found to predominate, the main fatty acids in the lamprey brain are presented by stearic (43-49% of total fatty acids) and oleic ones. N-acetylneuraminic acid is the only sialic acid found in gangliosides from the lamprey brain. Other components of ganglioside molecules are glucose, galactose and N-acetylgalactosamine. Two main lamprey brain gangliosides which constitute more than 90% of lipid-bound sialic acid, were found to be trisialogangliosides. It was shown that both gangliosides have the following structure in their molecules: (formula see text). They differ in the position of two other sialic acid residues. Data on lamprey brain gangliosides in the literature are practically absent. The data obtained in the present study confirmed the conclusions on changes in the hydrophobic part of ganglioside molecule in evolution of vertebrates and made it possible to define more exactly the molecular evolution of ganglioside carbohydrate component."}
{"id": "PMID:473996", "title": "[Histochemical study of cerebral cortex cholinesterases during early mammalian ontogeny].", "content": "Histochemical studies have been made on changes in true cholinesterase and pseudocholinesterase in mammalian ontogenesis. It was found that at early ontogenetic stages the enzymes are located mainly in deep layers. In rabbits, early accumulation of the true cholinesterase takes place; evident maximum of the reaction was found directly over the fibres of the cortical white matter during the last days of foetal development and in newborn animals. Accumulation of pseudocholinesterase in myelinating fibers begins at the 7-10th day. In the cortex of human foetuses, evident heterochronous accumulation of cholinesterase was noted, since the enzyme was concentrated mainly in the occipital and frontal parts of the isocortex.", "contents": "[Histochemical study of cerebral cortex cholinesterases during early mammalian ontogeny]. Histochemical studies have been made on changes in true cholinesterase and pseudocholinesterase in mammalian ontogenesis. It was found that at early ontogenetic stages the enzymes are located mainly in deep layers. In rabbits, early accumulation of the true cholinesterase takes place; evident maximum of the reaction was found directly over the fibres of the cortical white matter during the last days of foetal development and in newborn animals. Accumulation of pseudocholinesterase in myelinating fibers begins at the 7-10th day. In the cortex of human foetuses, evident heterochronous accumulation of cholinesterase was noted, since the enzyme was concentrated mainly in the occipital and frontal parts of the isocortex."}
{"id": "PMID:474002", "title": "[Microflora of the bronchial contents in chronic lung diseases].", "content": "The parallel bacteriological and mycological study of the contents of the bronchi in patients with various forms of chronic pneumonia provided a clearer picture of the causative agents of the disease. The composition of microflora in the bronchial contents corresponded to the severity of the process. Uncomplicated chronic pneumonia took its course with a bacterial monoculture, belonging predominantly to the family of pyogenic cocci, present in the discharges. The formation of abscesses and the development of bronchioectases proceeded of bacterial and mycotic associations. The development of malignant tumors was accompanied by the colonization of the pulmonary ways by a variety of organisms, including bacteria of the fecal flora.", "contents": "[Microflora of the bronchial contents in chronic lung diseases]. The parallel bacteriological and mycological study of the contents of the bronchi in patients with various forms of chronic pneumonia provided a clearer picture of the causative agents of the disease. The composition of microflora in the bronchial contents corresponded to the severity of the process. Uncomplicated chronic pneumonia took its course with a bacterial monoculture, belonging predominantly to the family of pyogenic cocci, present in the discharges. The formation of abscesses and the development of bronchioectases proceeded of bacterial and mycotic associations. The development of malignant tumors was accompanied by the colonization of the pulmonary ways by a variety of organisms, including bacteria of the fecal flora."}
{"id": "PMID:473992", "title": "[Visual projections in to the brain of the sturgeon Acipenser g\u00fcldenst\u00e4dti].", "content": "Visual projections have been studied after unilateral lesion of the optic nerve. Degenerated fibers and terminals, as well as fibers labelled by horseradish peroxidase were found contralaterally in the lateral geniculate thalamic nucleus, the second (stratum opticum) and the third (stratum fibrosum et griseum superficiale) layers of the midbrain tectum. In other brain structures (lateral ventrothalamic nucleus, in the pretectal area--cortical nucleus, pretectal nucleus, posterior comissure nucleus) the density of degeneration of optic fibers was significantly lower. Separate fragments of degeneration were found in the fourth layer of the tectum. Ipsilaterally, thalamic, pretectal and tectal visual projections of the same localization were found, although they were less massive than at the opposite side.", "contents": "[Visual projections in to the brain of the sturgeon Acipenser g\u00fcldenst\u00e4dti]. Visual projections have been studied after unilateral lesion of the optic nerve. Degenerated fibers and terminals, as well as fibers labelled by horseradish peroxidase were found contralaterally in the lateral geniculate thalamic nucleus, the second (stratum opticum) and the third (stratum fibrosum et griseum superficiale) layers of the midbrain tectum. In other brain structures (lateral ventrothalamic nucleus, in the pretectal area--cortical nucleus, pretectal nucleus, posterior comissure nucleus) the density of degeneration of optic fibers was significantly lower. Separate fragments of degeneration were found in the fourth layer of the tectum. Ipsilaterally, thalamic, pretectal and tectal visual projections of the same localization were found, although they were less massive than at the opposite side."}
{"id": "PMID:474003", "title": "[RNAse activity as an indirect index of the enterotoxigenicity of staphylococci].", "content": "On the basis of investigations carried out there was revealed a direct relationship between the capacity of staphylococci to produce enterotoxin and the extent of their RNA-ase activity. The activity of RNA-ase can serve as a significant indirect sign pointing to the enterotoxigenicity of staphylococcus.", "contents": "[RNAse activity as an indirect index of the enterotoxigenicity of staphylococci]. On the basis of investigations carried out there was revealed a direct relationship between the capacity of staphylococci to produce enterotoxin and the extent of their RNA-ase activity. The activity of RNA-ase can serve as a significant indirect sign pointing to the enterotoxigenicity of staphylococcus."}
{"id": "PMID:473999", "title": "[Electrographic changes in isolated cat cortex under the influence of sleep neuropeptide].", "content": "Experiments on adult cats with isolated cerebral cortex show that suboccipital olygopeptide administration (10 and 15 mg/kg) induces electrographic manifestation of the slow wave sleep (SWS) in the isolated cortex. Slow synchronous waves reflecting the onset of SWS appear in the isolated cortex 0.2-1.5 sec earlier than in the control hemisphere. The same administration prevents the onset of paradoxical sleep (PS) up to 2 hours from the beginning of the experiment. In all experiments, manifestation of the PS was recorded in both hemispheres simultaneously.", "contents": "[Electrographic changes in isolated cat cortex under the influence of sleep neuropeptide]. Experiments on adult cats with isolated cerebral cortex show that suboccipital olygopeptide administration (10 and 15 mg/kg) induces electrographic manifestation of the slow wave sleep (SWS) in the isolated cortex. Slow synchronous waves reflecting the onset of SWS appear in the isolated cortex 0.2-1.5 sec earlier than in the control hemisphere. The same administration prevents the onset of paradoxical sleep (PS) up to 2 hours from the beginning of the experiment. In all experiments, manifestation of the PS was recorded in both hemispheres simultaneously."}
{"id": "PMID:474006", "title": "[Use of the A-2 elective medium for the differentiation of Aeromonas from related microorganisms].", "content": "The trial of culture medium A-2, proposed for the identification of Aeromonas on the basis of the nutritional utilization of gelatin as the only source of nitrogen and starch as the source of carbon in view of the limited content of mineral components in the medium, revealed that no other species of the family Vibronaceae grew in this medium, and it could be used in an additional test for the differentiation of vibrios from Aeromonas. A group of strains isolated from human feces, surface water and sewage was found to be incapable of growing in culture medium A-2; these strains, though having a number of characteristics in common with Aeromonas, differed from them in a number of additional tests.", "contents": "[Use of the A-2 elective medium for the differentiation of Aeromonas from related microorganisms]. The trial of culture medium A-2, proposed for the identification of Aeromonas on the basis of the nutritional utilization of gelatin as the only source of nitrogen and starch as the source of carbon in view of the limited content of mineral components in the medium, revealed that no other species of the family Vibronaceae grew in this medium, and it could be used in an additional test for the differentiation of vibrios from Aeromonas. A group of strains isolated from human feces, surface water and sewage was found to be incapable of growing in culture medium A-2; these strains, though having a number of characteristics in common with Aeromonas, differed from them in a number of additional tests."}
{"id": "PMID:473997", "title": "[Trophic influence of the nervous system on the heat resistance of skeletal muscles in rats].", "content": "Chronic denervation of m. extensor digitorum longus in rats significantly increase the heat resistance of muscle fibers. This effect does not result from muscle disuse, since tenotomy does not affect the level of heat resistance of muscle fibers. After bilateral adrenalectomy, the thermostability of muscles remains unaffected. The data obtained are discussed in relation to the problem of coupled changes in thermostability and electrogenesis of muscles fibers in animal evolution.", "contents": "[Trophic influence of the nervous system on the heat resistance of skeletal muscles in rats]. Chronic denervation of m. extensor digitorum longus in rats significantly increase the heat resistance of muscle fibers. This effect does not result from muscle disuse, since tenotomy does not affect the level of heat resistance of muscle fibers. After bilateral adrenalectomy, the thermostability of muscles remains unaffected. The data obtained are discussed in relation to the problem of coupled changes in thermostability and electrogenesis of muscles fibers in animal evolution."}
{"id": "PMID:474008", "title": "[Yeast NaRNA as a stimulator of the virulence, immunogenicity and antigenicity of salmonellae].", "content": "Experiments on a great number of animals with the use of various methods showed that yeast NaRNA was capable of stimulating the increase of virulence, immunogenicity and antigenicity in S. typhi, the acceleration of their proliferation and growth, the accumulation of RNA and nucleotides without increasing their toxigenicity.", "contents": "[Yeast NaRNA as a stimulator of the virulence, immunogenicity and antigenicity of salmonellae]. Experiments on a great number of animals with the use of various methods showed that yeast NaRNA was capable of stimulating the increase of virulence, immunogenicity and antigenicity in S. typhi, the acceleration of their proliferation and growth, the accumulation of RNA and nucleotides without increasing their toxigenicity."}
{"id": "PMID:474009", "title": "[Antibacterial and antiviral activity of the polychloroethyl- and beta,beta-dichlorovinylamides of carboxylic acids].", "content": "A number of compounds having antiviral and antibacterial (antistaphylococcal) activity was found in the beta,beta-dichlorvinyl- and alpha-chlor(alpha-oxy)-beta,beta,beta-trichlorethylamide series of carboxylic acids. The antistaphylococcal activity of the compounds under study was found to depend, to a certain extent, ontheir chemical structure, whereas no such dependence was established in respect of their antiviral activity. In the treatment of combined influenza-staphylococcal infection a decrease in antistaphylococcal effect was observed, while antiviral activity remained stable.", "contents": "[Antibacterial and antiviral activity of the polychloroethyl- and beta,beta-dichlorovinylamides of carboxylic acids]. A number of compounds having antiviral and antibacterial (antistaphylococcal) activity was found in the beta,beta-dichlorvinyl- and alpha-chlor(alpha-oxy)-beta,beta,beta-trichlorethylamide series of carboxylic acids. The antistaphylococcal activity of the compounds under study was found to depend, to a certain extent, ontheir chemical structure, whereas no such dependence was established in respect of their antiviral activity. In the treatment of combined influenza-staphylococcal infection a decrease in antistaphylococcal effect was observed, while antiviral activity remained stable."}
{"id": "PMID:473998", "title": "[Water and electrolytes in the adductor of the mussel Mytilus edulis during adaptation to decreased salinity in vivo and in vitro].", "content": "To investigate the role of neurohumoral factors in acclimation of mussel muscle to a lowered salinity, studies have been made on the reaction of the intact mussel muscle and that of isolated muscle to change in the salinity from 26% to 10%. The general pattern of swelling of the adductor and the dynamics of its electrolyte composition in intact molluscs are similar to those in the isolated muscle. In the intact mussel this reaction of the adductor is slower. Acclimation of adductor cells is of the autonomic nature and does not depend on neurohumoral influences.", "contents": "[Water and electrolytes in the adductor of the mussel Mytilus edulis during adaptation to decreased salinity in vivo and in vitro]. To investigate the role of neurohumoral factors in acclimation of mussel muscle to a lowered salinity, studies have been made on the reaction of the intact mussel muscle and that of isolated muscle to change in the salinity from 26% to 10%. The general pattern of swelling of the adductor and the dynamics of its electrolyte composition in intact molluscs are similar to those in the isolated muscle. In the intact mussel this reaction of the adductor is slower. Acclimation of adductor cells is of the autonomic nature and does not depend on neurohumoral influences."}
{"id": "PMID:474010", "title": "[Effect of an autovaccine on the course of an experimental staphylococcal infection].", "content": "The effect of autovaccine on the state of cellular immunity in mice with staphylococcal infection was studied. The maximum decrease of staphylococcal dissemination in internal organs, espeically in the lungs, as well as an increase in the intensity of phagocytosis by peritoneal macrophages were observed after the administration of the vaccine by the method of inhalation. The intranasal administration of the vaccine also proved to be more effective than subcutaneous injection. The cumulation of immune response was more pronounced after the aerosol administration of autovaccine, especially in cases of pathological processes in the respiratory organs.", "contents": "[Effect of an autovaccine on the course of an experimental staphylococcal infection]. The effect of autovaccine on the state of cellular immunity in mice with staphylococcal infection was studied. The maximum decrease of staphylococcal dissemination in internal organs, espeically in the lungs, as well as an increase in the intensity of phagocytosis by peritoneal macrophages were observed after the administration of the vaccine by the method of inhalation. The intranasal administration of the vaccine also proved to be more effective than subcutaneous injection. The cumulation of immune response was more pronounced after the aerosol administration of autovaccine, especially in cases of pathological processes in the respiratory organs."}
{"id": "PMID:474011", "title": "[Biological properties of antibiotic-resistant strains of lactic acid bacteria].", "content": "Lactic-acid bacteria (L. fermenti, L. acidophilus, L. delbruecki), when developing resistance to antibiotics, did not change their main biochemical, antagonistic properties and did not lose capacity for acid production. Only a decrease in their growth rate and a change in their sensitivity to the action of ultraviolet radiation were observed. Both initial and antibiotic-resistant strains were capable of taking on the mucous membrane of the large and small intestines in CBA mice.", "contents": "[Biological properties of antibiotic-resistant strains of lactic acid bacteria]. Lactic-acid bacteria (L. fermenti, L. acidophilus, L. delbruecki), when developing resistance to antibiotics, did not change their main biochemical, antagonistic properties and did not lose capacity for acid production. Only a decrease in their growth rate and a change in their sensitivity to the action of ultraviolet radiation were observed. Both initial and antibiotic-resistant strains were capable of taking on the mucous membrane of the large and small intestines in CBA mice."}
{"id": "PMID:474012", "title": "[Spectral and integral-temporal analysis of cerebral evoked potentials in epilepsy patients].", "content": "Changes in the spectral and integral-temporal characteristics of evoked potentials (EP) to flashes, were studied in 17 epileptic patients and 20 normals. The analysis of EP was done on a background recording and after a 2-minute hyperventilation. It was demonstrated that an epileptization of the brain according to the integral indices of EP is expressed by an increase in the square response, duration of EP (an increase in the duration of the system return to a state of equilibrium), by a higher level of residual noise in a discharge of EP, as well as by an increase in the negative part of EP and a larger instability of responses. The spectral content of EP in epileptic patients demonstrates an increase in amplitude maxima, especially within theta = psi delta ranges. Hyperventilation in epileptic patients evokes a pronounced synchronization of brain bioelectric activity, which leads to a drop of the response intensity and an increase in the level of residual noise in a discharge of EP. The data obtained suggest that the proposed method of EP analysis may be effective in the determination of the intensity in the focus of epileptical activity, as well as in the study of the function of inhibitory mechanisms of convulsive discharges.", "contents": "[Spectral and integral-temporal analysis of cerebral evoked potentials in epilepsy patients]. Changes in the spectral and integral-temporal characteristics of evoked potentials (EP) to flashes, were studied in 17 epileptic patients and 20 normals. The analysis of EP was done on a background recording and after a 2-minute hyperventilation. It was demonstrated that an epileptization of the brain according to the integral indices of EP is expressed by an increase in the square response, duration of EP (an increase in the duration of the system return to a state of equilibrium), by a higher level of residual noise in a discharge of EP, as well as by an increase in the negative part of EP and a larger instability of responses. The spectral content of EP in epileptic patients demonstrates an increase in amplitude maxima, especially within theta = psi delta ranges. Hyperventilation in epileptic patients evokes a pronounced synchronization of brain bioelectric activity, which leads to a drop of the response intensity and an increase in the level of residual noise in a discharge of EP. The data obtained suggest that the proposed method of EP analysis may be effective in the determination of the intensity in the focus of epileptical activity, as well as in the study of the function of inhibitory mechanisms of convulsive discharges."}
{"id": "PMID:474013", "title": "[\"Forced normalization\" EEG phenomenon and several mechanisms of psychopathologic symptoms in epileptic patients].", "content": "Some electrophysiological correlations of periodically exacerbating psychopathological manifestations in epilepsy are discussed. It was established that in phenomenon of a \"forced normalization\" of the EEG deep structures of the lymbic system may show active foci of epileptical bioelectrical activity. It is suggested that such pictures of bioelectrical activity in the different brain structures are formed as a result of dissociated inhibitory influences of the brain stem. The necessity of a stereoelectroencephalographic study in this group of patients is emphasized in order to assess the possibilities of a successful surgical operation.", "contents": "[\"Forced normalization\" EEG phenomenon and several mechanisms of psychopathologic symptoms in epileptic patients]. Some electrophysiological correlations of periodically exacerbating psychopathological manifestations in epilepsy are discussed. It was established that in phenomenon of a \"forced normalization\" of the EEG deep structures of the lymbic system may show active foci of epileptical bioelectrical activity. It is suggested that such pictures of bioelectrical activity in the different brain structures are formed as a result of dissociated inhibitory influences of the brain stem. The necessity of a stereoelectroencephalographic study in this group of patients is emphasized in order to assess the possibilities of a successful surgical operation."}
{"id": "PMID:474014", "title": "[Role of the acetylcholine--cholinesterase system in the development of epilepsy].", "content": "The report pertains to some data on the cholinesterase activity in the blood serum and CSF of 62 patients with epilepsy, in correlation with different clinical characteristics (the severity of the disease, the character of the EEG, frequency of seizures, treatment efficacy, etc). In 86,8% of the cases there was a significant increase in the activity of serum cholinesterase. Increased cholinesterase activity correlated only with pronounced pathological changes in the EEG (reverse correlation) and the efficacy of treatment (direct correlation). After surgical treatment of 9 cases there was a drop in the cholinesterase activity of the blood serum and CSF, which correlated with an improvement in the general state of the patients. On the basis of personal experience, as well as literary data, it is assumed that an increase in the cholinesterase activity in epileptic patients is not related to the main etiological factors of this disease but is rather a secondary change, a peculiar \"symptom\" of the disease.", "contents": "[Role of the acetylcholine--cholinesterase system in the development of epilepsy]. The report pertains to some data on the cholinesterase activity in the blood serum and CSF of 62 patients with epilepsy, in correlation with different clinical characteristics (the severity of the disease, the character of the EEG, frequency of seizures, treatment efficacy, etc). In 86,8% of the cases there was a significant increase in the activity of serum cholinesterase. Increased cholinesterase activity correlated only with pronounced pathological changes in the EEG (reverse correlation) and the efficacy of treatment (direct correlation). After surgical treatment of 9 cases there was a drop in the cholinesterase activity of the blood serum and CSF, which correlated with an improvement in the general state of the patients. On the basis of personal experience, as well as literary data, it is assumed that an increase in the cholinesterase activity in epileptic patients is not related to the main etiological factors of this disease but is rather a secondary change, a peculiar \"symptom\" of the disease."}
{"id": "PMID:474015", "title": "[Hemostasis in epileptic seizures and status epilepticus].", "content": "The hemostasis function was studied in 33 patients with epileptical seizures of a different etiology. The studies were conducted at the moment of the epileptical seizure or epileptical status and in the interattack period during hyperventilation. The concentration of products of blood fibrinogen degradation, the degree of platelet agregation, their number and prothrombine index were estimated. The data obtained were compared with those of normals. The results of the studies indicate the existence of the syndrome of intravascular coagulation during an epileptical seizure and epileptical status.", "contents": "[Hemostasis in epileptic seizures and status epilepticus]. The hemostasis function was studied in 33 patients with epileptical seizures of a different etiology. The studies were conducted at the moment of the epileptical seizure or epileptical status and in the interattack period during hyperventilation. The concentration of products of blood fibrinogen degradation, the degree of platelet agregation, their number and prothrombine index were estimated. The data obtained were compared with those of normals. The results of the studies indicate the existence of the syndrome of intravascular coagulation during an epileptical seizure and epileptical status."}
{"id": "PMID:474016", "title": "[Problem of focal pathology in \"genuine\" epilepsy].", "content": "Chronic experiments on rats permitted one to obtain an experimental model of photogenic epilepsy. This was accomplished by creating in the external geniculate body (EGB) a generator of pathologically intensified excitation (GPIE) with the aid of tetanus toxin (TT). According to some clinical and electrographic characteristics this form of epilepsy may be alloted to the type of primarily generalized or \"genuine\" epilepsy. The existance of focal pathology in the respective EGB was detected only by special electrophysiological and pharmacological methods. The studied model demonstrates a necessity of using special methods of detecting brain focal pathology (GPIE) in order to determine the focus of neuron hyperactivity in those cases where the clinical picture is characterized by signs of the so-called genuine epilepsy. Experimental photogenic epilepsy attained by creating GPIE in the EGB with the aid of TT, is proposed as a model for studying the mechanism of epileptogenesis and testing the efficacy of anticonvulsive drugs.", "contents": "[Problem of focal pathology in \"genuine\" epilepsy]. Chronic experiments on rats permitted one to obtain an experimental model of photogenic epilepsy. This was accomplished by creating in the external geniculate body (EGB) a generator of pathologically intensified excitation (GPIE) with the aid of tetanus toxin (TT). According to some clinical and electrographic characteristics this form of epilepsy may be alloted to the type of primarily generalized or \"genuine\" epilepsy. The existance of focal pathology in the respective EGB was detected only by special electrophysiological and pharmacological methods. The studied model demonstrates a necessity of using special methods of detecting brain focal pathology (GPIE) in order to determine the focus of neuron hyperactivity in those cases where the clinical picture is characterized by signs of the so-called genuine epilepsy. Experimental photogenic epilepsy attained by creating GPIE in the EGB with the aid of TT, is proposed as a model for studying the mechanism of epileptogenesis and testing the efficacy of anticonvulsive drugs."}
{"id": "PMID:474017", "title": "[Pain phenomenon in the structure of a seizure as a symptom of subcortical epilepsy].", "content": "A long-term dynamic study of epilepsy permitted one to distinguish a group of patients (21), who had undergone cortical surgical operations in the sensomotor area. In this group of patients the epileptical seizures were not only arrested, but preserved their previous structure. In all observations, the attack included pain which had a nociceptive character, a disagreeable emotional shading, was exceptionally intensive, diffuse, spreaded along a wide body area, was localized in the deep tissues and migrated intermittently during the seizure, respective to the somatotopic representation in the brain. The anatomo-physiological data suggest that such painful sensations are due to the irritation of the nonspecific thalamus nuclei and first of all, the medium center. This assumption is supported by the clinico-physiological studies of some authors, as well as by the negative results of surgical operations in this group of patients, despite the removal of the assumed epileptical focus in the cortex. The studies indicate that the main epileptical focus lies in the subcortical structures and particularly in the nonspecific thalamic nuclei.", "contents": "[Pain phenomenon in the structure of a seizure as a symptom of subcortical epilepsy]. A long-term dynamic study of epilepsy permitted one to distinguish a group of patients (21), who had undergone cortical surgical operations in the sensomotor area. In this group of patients the epileptical seizures were not only arrested, but preserved their previous structure. In all observations, the attack included pain which had a nociceptive character, a disagreeable emotional shading, was exceptionally intensive, diffuse, spreaded along a wide body area, was localized in the deep tissues and migrated intermittently during the seizure, respective to the somatotopic representation in the brain. The anatomo-physiological data suggest that such painful sensations are due to the irritation of the nonspecific thalamus nuclei and first of all, the medium center. This assumption is supported by the clinico-physiological studies of some authors, as well as by the negative results of surgical operations in this group of patients, despite the removal of the assumed epileptical focus in the cortex. The studies indicate that the main epileptical focus lies in the subcortical structures and particularly in the nonspecific thalamic nuclei."}
{"id": "PMID:474018", "title": "[Epileptic seizures in stenosis and de-stenosis of the common carotid arteries].", "content": "Experiments were performed in 64 white laboratory rats sensitive to acoustic stimula with disturbances and a restitution of cerebral circulation due to stenosis and destenosis of the common carotid arteries. The epileptic reaction of rats was studied prior to and following stenosis and destanosis by means of audiogenic epilepsy. In highly sensitive rats of the KM strain with a leftside stenosis of the common carotid arteries the epileptical reaction did not change significantly. In rats, selected from a random population after a simultaneous bilateral stenosis of the common carotid arteries there was an intensification of epileptical seizures in 70,6%. The restitution of the blood flow after 10 days following stenosis of the common carotid arteries facilitated a drop of the convulsive reaction. In animals who were not operated for the restitution of the blood flow in the common carotid arteries the reaction remained high even after 3 months following disturbances of cerebral circulation.", "contents": "[Epileptic seizures in stenosis and de-stenosis of the common carotid arteries]. Experiments were performed in 64 white laboratory rats sensitive to acoustic stimula with disturbances and a restitution of cerebral circulation due to stenosis and destenosis of the common carotid arteries. The epileptic reaction of rats was studied prior to and following stenosis and destanosis by means of audiogenic epilepsy. In highly sensitive rats of the KM strain with a leftside stenosis of the common carotid arteries the epileptical reaction did not change significantly. In rats, selected from a random population after a simultaneous bilateral stenosis of the common carotid arteries there was an intensification of epileptical seizures in 70,6%. The restitution of the blood flow after 10 days following stenosis of the common carotid arteries facilitated a drop of the convulsive reaction. In animals who were not operated for the restitution of the blood flow in the common carotid arteries the reaction remained high even after 3 months following disturbances of cerebral circulation."}
{"id": "PMID:474019", "title": "[Present state and future tasks for gerontopsychiatric research].", "content": "The state of scientific studies in gerontopsychiatry in the USSR is characterized. The development of respective epidemiological and follow-up studies is positively assessed. However such global problems related to studies in organizational and basic problems (biological, neurophysiological, genetical, pathopsychological, etc) are not sufficiently represented. It is emphasized that new approaches in gerontopsychiatry such as the study of borderline mental disorders in the elderly, the role of social and microsocial factors, the elaboration of psychotherapeutical and rehabilitative measures are developing.", "contents": "[Present state and future tasks for gerontopsychiatric research]. The state of scientific studies in gerontopsychiatry in the USSR is characterized. The development of respective epidemiological and follow-up studies is positively assessed. However such global problems related to studies in organizational and basic problems (biological, neurophysiological, genetical, pathopsychological, etc) are not sufficiently represented. It is emphasized that new approaches in gerontopsychiatry such as the study of borderline mental disorders in the elderly, the role of social and microsocial factors, the elaboration of psychotherapeutical and rehabilitative measures are developing."}
{"id": "PMID:474020", "title": "[Visuospatial thought disorders in patients with localized brain lesions].", "content": "The paper is concerned with the problem of disturbed visual-spatial thinking in patients with focal brain lesions. As is known, visual-spatial thinking can be disturbed in patients with lesions of the occipital, parieto-occipital and frontal lobes. However, the character of these disturbances is different. Attempts are made to examine these differences in varying localizations of the lesions. The results obtained demonstrate that disturbed visual-spatial thinking in different groups of patients is underlain by both common and specific factors.", "contents": "[Visuospatial thought disorders in patients with localized brain lesions]. The paper is concerned with the problem of disturbed visual-spatial thinking in patients with focal brain lesions. As is known, visual-spatial thinking can be disturbed in patients with lesions of the occipital, parieto-occipital and frontal lobes. However, the character of these disturbances is different. Attempts are made to examine these differences in varying localizations of the lesions. The results obtained demonstrate that disturbed visual-spatial thinking in different groups of patients is underlain by both common and specific factors."}
{"id": "PMID:474021", "title": "[Neurochemical basis of the pathogenesis of different types of addiction].", "content": "The study is concerned with the influence of narcotic drugs of a different chemical nature (alcohol, morphine, barbamil) on the catecholamine metabolism in some areas of the brain and adrenal gland in experimental animals. Each of the studied drugs exerted a differentiated effect on the catecholamine system in the different brain structures. However all these substances both in a single administration and in a long-term use, and development of a drug dependence, evoked similar changes in the catecholamine metabolism in the hypothalamus and in the middle brain. These changes were shown by an intensified release and destruction of the noradrenalin neuromediator under the influence of the above-mentioned substances and a compensatory increases in the catecholamine synthesis on a prolonged use. Withdrawal of narcotic drugs leads to a significant increase in the dofamine level in these brain areas. The pharmacological analysis demonstrated, that the formation of a narcotic dependence may be connected with the changed functions of the catecholamine brain system.", "contents": "[Neurochemical basis of the pathogenesis of different types of addiction]. The study is concerned with the influence of narcotic drugs of a different chemical nature (alcohol, morphine, barbamil) on the catecholamine metabolism in some areas of the brain and adrenal gland in experimental animals. Each of the studied drugs exerted a differentiated effect on the catecholamine system in the different brain structures. However all these substances both in a single administration and in a long-term use, and development of a drug dependence, evoked similar changes in the catecholamine metabolism in the hypothalamus and in the middle brain. These changes were shown by an intensified release and destruction of the noradrenalin neuromediator under the influence of the above-mentioned substances and a compensatory increases in the catecholamine synthesis on a prolonged use. Withdrawal of narcotic drugs leads to a significant increase in the dofamine level in these brain areas. The pharmacological analysis demonstrated, that the formation of a narcotic dependence may be connected with the changed functions of the catecholamine brain system."}
{"id": "PMID:474022", "title": "[Significance of several factors for actualization of the pathologic drive to alcohol in chronic alcoholism].", "content": "On the basis of literary data and clinical experience, a list of 27 factors was compiled, which could have a significance for actualizing pathological drives to alcohol in chronic alcoholism. The factors were marked under such headings as \"individual\", \"family-environmental\" and \"connected with the environment at work\". In order to establish the connection of each factor with the actualization of a pathological drive to alcohol, 200 inpatients treated for chronic alcoholism were examined. A comparative analysis was performed for eliminating the significance of factors for groups of patients with different stages of the disease and premorbid personality types. The possibility of using these data in clinical practice and preventing relapses in the treatment of chronic alcoholism is assessed.", "contents": "[Significance of several factors for actualization of the pathologic drive to alcohol in chronic alcoholism]. On the basis of literary data and clinical experience, a list of 27 factors was compiled, which could have a significance for actualizing pathological drives to alcohol in chronic alcoholism. The factors were marked under such headings as \"individual\", \"family-environmental\" and \"connected with the environment at work\". In order to establish the connection of each factor with the actualization of a pathological drive to alcohol, 200 inpatients treated for chronic alcoholism were examined. A comparative analysis was performed for eliminating the significance of factors for groups of patients with different stages of the disease and premorbid personality types. The possibility of using these data in clinical practice and preventing relapses in the treatment of chronic alcoholism is assessed."}
{"id": "PMID:474023", "title": "[Clinical picture of atypical alcoholic psychoses].", "content": "Results of a long-term clinico-catamnestical study of 489 patients with atypical alcoholic psychoses indicate that only the following cases develop atypically; a) where in the framework of separate attacks there is a combination of verbal hallucinosis, diverse more or less systematized delusional ideas (including delusions of influence), separate traits of the syndrome of psychic automatisms and oneiroid episodes; b) where there is a transformation (atypization) of the syndromokinesis from attack to attack; c) where there is a continuation of the process of atypization up to a development of signs of partial or total alcoholic or alcoholic-organic dementia.", "contents": "[Clinical picture of atypical alcoholic psychoses]. Results of a long-term clinico-catamnestical study of 489 patients with atypical alcoholic psychoses indicate that only the following cases develop atypically; a) where in the framework of separate attacks there is a combination of verbal hallucinosis, diverse more or less systematized delusional ideas (including delusions of influence), separate traits of the syndrome of psychic automatisms and oneiroid episodes; b) where there is a transformation (atypization) of the syndromokinesis from attack to attack; c) where there is a continuation of the process of atypization up to a development of signs of partial or total alcoholic or alcoholic-organic dementia."}
{"id": "PMID:474024", "title": "[Several features of the course of attack-like schizophrenia (according to the findings of an epidemiologic study)].", "content": "The authors studied patients with an attack-like course and a \"cliche\" type course (i.e. the psychopathological structure of attacks does not change within the disease). In spite of the differences in the clinical picture of the attacks, the studied patients slightly differed in age, onset of the 1st attack, number of sick male- and female patients, correlation between the age at the onset of the 1st attack and sex, number of previous attacks and continuation of remissions. A direct correlational dependance between the character of productive disturbances and the depth of negative changes in remissions and the clinical picture of psychotic attacks was observed.", "contents": "[Several features of the course of attack-like schizophrenia (according to the findings of an epidemiologic study)]. The authors studied patients with an attack-like course and a \"cliche\" type course (i.e. the psychopathological structure of attacks does not change within the disease). In spite of the differences in the clinical picture of the attacks, the studied patients slightly differed in age, onset of the 1st attack, number of sick male- and female patients, correlation between the age at the onset of the 1st attack and sex, number of previous attacks and continuation of remissions. A direct correlational dependance between the character of productive disturbances and the depth of negative changes in remissions and the clinical picture of psychotic attacks was observed."}
{"id": "PMID:474025", "title": "[Typology of status upon discharge from a psychiatric hospital and evaluation of the effectiveness of treating patients with endogenous psychoses].", "content": "A new form of evaluating the state (formula) in patients with endogenous psychoses when being discharged from the mental hospital is proposed. This formula contains a differentiated estimation of the clinical and socio-working state, as well as a list of risk factors for relapses, treatment complications, social deadaptation and an assessment of the treatment efficacy. This form is directed towards a perfection of the continuity between inpatient and outpatient mental services in the treatment of patients.", "contents": "[Typology of status upon discharge from a psychiatric hospital and evaluation of the effectiveness of treating patients with endogenous psychoses]. A new form of evaluating the state (formula) in patients with endogenous psychoses when being discharged from the mental hospital is proposed. This formula contains a differentiated estimation of the clinical and socio-working state, as well as a list of risk factors for relapses, treatment complications, social deadaptation and an assessment of the treatment efficacy. This form is directed towards a perfection of the continuity between inpatient and outpatient mental services in the treatment of patients."}
{"id": "PMID:474034", "title": "Study on the reproducibility of human prolactin response to sulpiride, benserazide, insulin hypoglycaemia and arginine infusion.", "content": "In order to evaluate the spontaneous variability of prolactin (PRL) release in response to various stimuli applied repeatedly on different occasions, groups of 5 to 12 subjects each underwent consecutive identical tests with one of the following stimuli applied at 3-6 days' intervals: sulpiride (100 mg im), benserazide (50 mg po), insulin hypoglycaemia (0.1 U/kg b. w. iv) and arginine infusion (25 g iv in 30 min). When repeated in the same subjects, arginine and benserazide yielded superimposable results. In contrast to this, insulin hypoglycaemia yielded significantly lower PRL release, while the PRL response to the second sulpiride test was significantly higher than to the first one. When an interval of 10 days was left between two consecutive sulpiride tests, an identical PRL release was observed. These results indicate that arginine and benserazide are reproducible tests for PRL secretion and it is possible that the decreasing effect of insulin hypoglycaemia on PRL release is due to the stressful effect of the stimulus. Finally, sulpiride probably enhances both PRL release and synthesis thus making greater amounts of PRL available to a subsequent stimulus. Since some of the above stimuli are usual tools for the study of the neuroendocrine control of PRL secretion, our findings suggest that caution appears necessary in attributing to any (neuroactive) drug an effect which might be merely due to a lack of reproducibility of the stimulus employed.", "contents": "Study on the reproducibility of human prolactin response to sulpiride, benserazide, insulin hypoglycaemia and arginine infusion. In order to evaluate the spontaneous variability of prolactin (PRL) release in response to various stimuli applied repeatedly on different occasions, groups of 5 to 12 subjects each underwent consecutive identical tests with one of the following stimuli applied at 3-6 days' intervals: sulpiride (100 mg im), benserazide (50 mg po), insulin hypoglycaemia (0.1 U/kg b. w. iv) and arginine infusion (25 g iv in 30 min). When repeated in the same subjects, arginine and benserazide yielded superimposable results. In contrast to this, insulin hypoglycaemia yielded significantly lower PRL release, while the PRL response to the second sulpiride test was significantly higher than to the first one. When an interval of 10 days was left between two consecutive sulpiride tests, an identical PRL release was observed. These results indicate that arginine and benserazide are reproducible tests for PRL secretion and it is possible that the decreasing effect of insulin hypoglycaemia on PRL release is due to the stressful effect of the stimulus. Finally, sulpiride probably enhances both PRL release and synthesis thus making greater amounts of PRL available to a subsequent stimulus. Since some of the above stimuli are usual tools for the study of the neuroendocrine control of PRL secretion, our findings suggest that caution appears necessary in attributing to any (neuroactive) drug an effect which might be merely due to a lack of reproducibility of the stimulus employed."}
{"id": "PMID:474035", "title": "Chlorpromazine inhibits episodic growth hormone secretion in the rat.", "content": "Effects of chlorpromazine (CPZ) on plasma GH and prolactin levels were observed in conscious rats provided with chronic indwelling right atrial cannulae. The administration of CPZ (200 micrograms/100 g b.w. iv) suppressed episodic plasma GH burst and resulted in significant elevations of plasma prolactin levels. These were also observed in rats in which two types of hypothalamic deafferentation, i.e. anterior and complete, had been carried out. The data suggest that CPZ acts within the medial basal hypothalamus and inhibits episodic plasma GH secretion. In addition, it is inferred that catecholamines are involved in the generation of episodic plasma GH burst.", "contents": "Chlorpromazine inhibits episodic growth hormone secretion in the rat. Effects of chlorpromazine (CPZ) on plasma GH and prolactin levels were observed in conscious rats provided with chronic indwelling right atrial cannulae. The administration of CPZ (200 micrograms/100 g b.w. iv) suppressed episodic plasma GH burst and resulted in significant elevations of plasma prolactin levels. These were also observed in rats in which two types of hypothalamic deafferentation, i.e. anterior and complete, had been carried out. The data suggest that CPZ acts within the medial basal hypothalamus and inhibits episodic plasma GH secretion. In addition, it is inferred that catecholamines are involved in the generation of episodic plasma GH burst."}
{"id": "PMID:474036", "title": "Osteoporosis in hyperthyroidism estimated by photon absorptiometry.", "content": "The degree of osteoporosis in hyperthyroidism before and during treatment with carbimazole was studied by photon absorption technique of the right forearm and calcaneus. In addition serum total calcium, serum ionized calcium, serum phosphorus and serum alkaline phosphatase were determined. A group of 96 patients suffering from untreated hyperthyroidism (85 women and 11 men) was studied (79 of these patients were also followed during treatment) and compared to a control group of 157 persons (107 women and 50 men). The women were divided into two groups: less than or equal to 45 years old and more than 45 years old. In all groups untreated hyperthyroid patients showed lower bone densities compared to the control group, but this was only significant in women. During treatment all groups showed a significant increase in density. After 3-6 months of treatment bone density in the calcaneus increased 12% and in the forearm 1.5%; after 6 months - 3 years 33% and 31%, respectively. At that time bone density was normalized. There was no correlation between bone density in hyperthyroid patients and duration and severity of the disease. The biochemical changes were characterised by increases in serum alkaline phosphatase (26%), serum total calcium (16%) and serum ionized calcium concentration (17%) in cases of untreated hyperthyroidism. Serum phosphorus concentration did not change. A correlation was found between elevation of the alkaline phosphatase and decreased bone density.", "contents": "Osteoporosis in hyperthyroidism estimated by photon absorptiometry. The degree of osteoporosis in hyperthyroidism before and during treatment with carbimazole was studied by photon absorption technique of the right forearm and calcaneus. In addition serum total calcium, serum ionized calcium, serum phosphorus and serum alkaline phosphatase were determined. A group of 96 patients suffering from untreated hyperthyroidism (85 women and 11 men) was studied (79 of these patients were also followed during treatment) and compared to a control group of 157 persons (107 women and 50 men). The women were divided into two groups: less than or equal to 45 years old and more than 45 years old. In all groups untreated hyperthyroid patients showed lower bone densities compared to the control group, but this was only significant in women. During treatment all groups showed a significant increase in density. After 3-6 months of treatment bone density in the calcaneus increased 12% and in the forearm 1.5%; after 6 months - 3 years 33% and 31%, respectively. At that time bone density was normalized. There was no correlation between bone density in hyperthyroid patients and duration and severity of the disease. The biochemical changes were characterised by increases in serum alkaline phosphatase (26%), serum total calcium (16%) and serum ionized calcium concentration (17%) in cases of untreated hyperthyroidism. Serum phosphorus concentration did not change. A correlation was found between elevation of the alkaline phosphatase and decreased bone density."}
{"id": "PMID:474028", "title": "[Therapeutic strategy in the cold nodule of the thyroid (author's transl)].", "content": "The benign or malignant nature of a cold nodule in the thyroid can never be definitely assessed before surgery. All cold nodules that show no tendency to regress must be resected. The nodule is resected with surrounding healthy tissue and immediately examined in freezen sections. Out of 108 cases of simple resection of the nodule only one diagnosis of carcinoma was missed at the operation. That patient was reoperated two weeks later with curative intention.", "contents": "[Therapeutic strategy in the cold nodule of the thyroid (author's transl)]. The benign or malignant nature of a cold nodule in the thyroid can never be definitely assessed before surgery. All cold nodules that show no tendency to regress must be resected. The nodule is resected with surrounding healthy tissue and immediately examined in freezen sections. Out of 108 cases of simple resection of the nodule only one diagnosis of carcinoma was missed at the operation. That patient was reoperated two weeks later with curative intention."}
{"id": "PMID:474037", "title": "The use of region specific radioimmunoassays for characterization of circulating calcitonin in patients with medullary carcinoma of the thyroid gland.", "content": "Two antisera with known region specificities have been used to characterize calcitonin immunoreactivity (iCT) in serum of patients with medullary thyroid carcinoma (MCT). Antiserum I which was raised against the synthetic hormone (1-32 amino acid residues), contained heterogeneous populations of immunoglobulins directed predominantly against carboxy-terminal sequences of the hormone, but the antiserum reacted also with the amino-terminal fragment (1-10 amino acid residues). Antiserum II, which was raised against the carboxy-terminal hormone fragment (11-32 amino acid residues) reached equally well with the intact hormone and the C-terminal fragment, but showed negligible binding of the amino terminal fragment. Antiserum I measured therefore both amino-terminal and carboxy-terminal sequences of calcitonin while antiserum II measured only carboxy-terminal amino acid sequences. In 40 patients with MCT, antiserum I measured usually the highest concentration of serum iCT suggesting the presence of non-uniform hormone immunoreactivity. The different molecular forms of circulating iCT in 7 MCT patients were explored by using antiserum I after gel filtration on Sephadex G-100. The patients who were selected on basis of iCT measurement in serum using antiserum I and II, could be divided into 3 groups which showed characteristic iCT profiles. Group 1, in which antiserum II measured a higher concentration of serum iCT, contained predominantly (60-70%) small fragments of calcitonin immunoreactivity. On the other hand, in the sera of group 3 in which antisera I measured an equal or the highest concentrations, the dominant form of the hormone consisted of molecular sequences equal to or larger than the intact hormone (90%). In group 2, the two antisera measured an equal amount of serum iCT and molecular forms consisting mostly of larger hormone fragments dominated (50%). All the patients were normocalcaemic in spite of frequently grossly elevated serum iCT, and 33 out of 36 patients had normal serum immunoreactive parathyroid hormone. 1. Serum iCT is heterogeneous and represents peptides of quite different molecular size with no or low biological activity. 2. Most of the serum calcitonin immunoreactivity consists of peptides with carboxy-terminal amino acid sequences. 3. Most, if not all, of the amino-terminal calcitonin immunoreactivity is due to monomeric and polymeric hormonal forms.", "contents": "The use of region specific radioimmunoassays for characterization of circulating calcitonin in patients with medullary carcinoma of the thyroid gland. Two antisera with known region specificities have been used to characterize calcitonin immunoreactivity (iCT) in serum of patients with medullary thyroid carcinoma (MCT). Antiserum I which was raised against the synthetic hormone (1-32 amino acid residues), contained heterogeneous populations of immunoglobulins directed predominantly against carboxy-terminal sequences of the hormone, but the antiserum reacted also with the amino-terminal fragment (1-10 amino acid residues). Antiserum II, which was raised against the carboxy-terminal hormone fragment (11-32 amino acid residues) reached equally well with the intact hormone and the C-terminal fragment, but showed negligible binding of the amino terminal fragment. Antiserum I measured therefore both amino-terminal and carboxy-terminal sequences of calcitonin while antiserum II measured only carboxy-terminal amino acid sequences. In 40 patients with MCT, antiserum I measured usually the highest concentration of serum iCT suggesting the presence of non-uniform hormone immunoreactivity. The different molecular forms of circulating iCT in 7 MCT patients were explored by using antiserum I after gel filtration on Sephadex G-100. The patients who were selected on basis of iCT measurement in serum using antiserum I and II, could be divided into 3 groups which showed characteristic iCT profiles. Group 1, in which antiserum II measured a higher concentration of serum iCT, contained predominantly (60-70%) small fragments of calcitonin immunoreactivity. On the other hand, in the sera of group 3 in which antisera I measured an equal or the highest concentrations, the dominant form of the hormone consisted of molecular sequences equal to or larger than the intact hormone (90%). In group 2, the two antisera measured an equal amount of serum iCT and molecular forms consisting mostly of larger hormone fragments dominated (50%). All the patients were normocalcaemic in spite of frequently grossly elevated serum iCT, and 33 out of 36 patients had normal serum immunoreactive parathyroid hormone. 1. Serum iCT is heterogeneous and represents peptides of quite different molecular size with no or low biological activity. 2. Most of the serum calcitonin immunoreactivity consists of peptides with carboxy-terminal amino acid sequences. 3. Most, if not all, of the amino-terminal calcitonin immunoreactivity is due to monomeric and polymeric hormonal forms."}
{"id": "PMID:474033", "title": "[Lymphedema after modified radical mastectomy as performed at the Bordet Institute. A report of 60 cases (author's transl)].", "content": "Three hundred and fifteen patients who underwent a radical mastectomy between 1969 and 1976 were studied. Nineteen percent developed lymphedema of the upper limb. Ten percent were resistant to all forms of therapy while the pathology gradually subsided in 9%. The causes of lymphedema as well as the preventive and curative methods were statistically studied and compared with those in the literature. Several preventive measures are proposed, in particular, the modified radical mastectomy.", "contents": "[Lymphedema after modified radical mastectomy as performed at the Bordet Institute. A report of 60 cases (author's transl)]. Three hundred and fifteen patients who underwent a radical mastectomy between 1969 and 1976 were studied. Nineteen percent developed lymphedema of the upper limb. Ten percent were resistant to all forms of therapy while the pathology gradually subsided in 9%. The causes of lymphedema as well as the preventive and curative methods were statistically studied and compared with those in the literature. Several preventive measures are proposed, in particular, the modified radical mastectomy."}
{"id": "PMID:474039", "title": "Effect of iodine intake and food consumption.", "content": "Rats were exposed to cold (4 degrees C) for 1 and 4 weeks. The T4 plasma concentrations initially declined (24.5 +/- 7.7 nmol/l) after 1 week but returned to normal levels after 4 weeks (52.9 +/- 14.2 nmol/l). The T3 concentrations were elevated after both 1 and 4 weeks at 4 degrees C (1.31 +/- 0.21 and 1.38 +/- 0.12 nmol/l, respectively). Control values (23 degrees C) for T4 were 42.6 +/- 10.3 and for T3 1.11 +/- 0.13 nmol/l. Addition of 0.015 g KI/l to the drinking water prevented the T4 decrease in plasma after 1 week of cold exposure. No effect of iodide was observed at 23 degrees C. The suppletion of KI did not change pattern of T3 increase after cold exposure. After 4 weeks of cold exposure the T4 levels of the iodide-supplemented animals did not differ from the non-sulemented group. No evidence was found that increased food intake is a contributory factor in the development leading to increased T3 plasma levels during cold exposure.", "contents": "Effect of iodine intake and food consumption. Rats were exposed to cold (4 degrees C) for 1 and 4 weeks. The T4 plasma concentrations initially declined (24.5 +/- 7.7 nmol/l) after 1 week but returned to normal levels after 4 weeks (52.9 +/- 14.2 nmol/l). The T3 concentrations were elevated after both 1 and 4 weeks at 4 degrees C (1.31 +/- 0.21 and 1.38 +/- 0.12 nmol/l, respectively). Control values (23 degrees C) for T4 were 42.6 +/- 10.3 and for T3 1.11 +/- 0.13 nmol/l. Addition of 0.015 g KI/l to the drinking water prevented the T4 decrease in plasma after 1 week of cold exposure. No effect of iodide was observed at 23 degrees C. The suppletion of KI did not change pattern of T3 increase after cold exposure. After 4 weeks of cold exposure the T4 levels of the iodide-supplemented animals did not differ from the non-sulemented group. No evidence was found that increased food intake is a contributory factor in the development leading to increased T3 plasma levels during cold exposure."}
{"id": "PMID:474029", "title": "[Mammary reconstruction after resection (author's transl)].", "content": "The authors present 44 cases of mammary reconstruction after sub-cutaneous mastectomies (16 cases), modified radical mastectomies (20 cases) and total radical mastectomies (8 cases). The implantation of a prosthesis is always performed a few months after sub-cutaneous mastectomy to avoid the numerous complications. Implantation of a prosthesis after a modified radical mastectomy (conservation of the pectoral muscles) yields excellent results. The authors graft the areola-nipple complex of the mastectomised breast on the abdominal skin, each time that they are sure of its integrity. At the later implantation of the mammary prosthesis, they perform a reduction or remodeling of the controlateral breast, if indicated. The total radical mastectomy without radiation generally allows implantation of a prosthesis without additional use of skin flaps or musculo-cutaneous skin flaps. Sequellea of radiodystrophy may impose preliminary cutaneous or musculo-cutaneous skinflaps with inferior esthetic results.", "contents": "[Mammary reconstruction after resection (author's transl)]. The authors present 44 cases of mammary reconstruction after sub-cutaneous mastectomies (16 cases), modified radical mastectomies (20 cases) and total radical mastectomies (8 cases). The implantation of a prosthesis is always performed a few months after sub-cutaneous mastectomy to avoid the numerous complications. Implantation of a prosthesis after a modified radical mastectomy (conservation of the pectoral muscles) yields excellent results. The authors graft the areola-nipple complex of the mastectomised breast on the abdominal skin, each time that they are sure of its integrity. At the later implantation of the mammary prosthesis, they perform a reduction or remodeling of the controlateral breast, if indicated. The total radical mastectomy without radiation generally allows implantation of a prosthesis without additional use of skin flaps or musculo-cutaneous skin flaps. Sequellea of radiodystrophy may impose preliminary cutaneous or musculo-cutaneous skinflaps with inferior esthetic results."}
{"id": "PMID:474040", "title": "Studies on the origin of altered thyroid hormone levels in the blood of rats during cold exposure. II. Effect of propranolol and chemical sympathectomy.", "content": "The effect of sympathetic activity on T4 and T3 levels in cold-exposed rats was investigated. Administration of the highest dose of propranolol (2 mg/100 g b.w.) twice daily during 4 days decreased T4 and T3 concentrations in plasma of rats living at 23 degrees C (T4 from 46.4 +/- 2.6 to 25.8 +/- 5.3 nmol/l and T3 from 1.08 +/- 0.6 to 0.82 +/- 0.12 nmol/l). No significant effect on T4 and T3 levels (49.0 +/- 11.6 and 1.48 +/- 0.16 n/mol, respectively) after the administration of the same dose regimen of propranolol was observed in rats exposed to cold for 4 weeks. T4 and T3 levels in rats exposed to cold for 4 weeks were not significantly altered 1 week after sympathectomy, while remaining in the cold. However, chemical sympathectomy before cold exposure delayed the cold induced T3 elevation occurring during the first week of cold exposure (controls: from 1.16 +/- 0.19 to 1.44 +/- 0.29 nmol/l; sympathectomized rats: from 1.07 +/- 0.12 to 1.17 +/- 0.22 nmol/l). After 2 weeks of cold exposure the T3 levels of controls and sympathectomized rats were not significantly different (controls: 1.45 +/- 0.12 nmol/l, sympathectomized  rats: 1.38 +/- 0.15 nmol/l). No effect of sympathectomy was observed on T4 levels. These experiments show that the role of sympathetic activity in increasing T3 is not clear during cold exposure. They provide some evidence that sympathetic activity may play a role in the initiation of the process leading to increased T3 plasma levels during cold exposure.", "contents": "Studies on the origin of altered thyroid hormone levels in the blood of rats during cold exposure. II. Effect of propranolol and chemical sympathectomy. The effect of sympathetic activity on T4 and T3 levels in cold-exposed rats was investigated. Administration of the highest dose of propranolol (2 mg/100 g b.w.) twice daily during 4 days decreased T4 and T3 concentrations in plasma of rats living at 23 degrees C (T4 from 46.4 +/- 2.6 to 25.8 +/- 5.3 nmol/l and T3 from 1.08 +/- 0.6 to 0.82 +/- 0.12 nmol/l). No significant effect on T4 and T3 levels (49.0 +/- 11.6 and 1.48 +/- 0.16 n/mol, respectively) after the administration of the same dose regimen of propranolol was observed in rats exposed to cold for 4 weeks. T4 and T3 levels in rats exposed to cold for 4 weeks were not significantly altered 1 week after sympathectomy, while remaining in the cold. However, chemical sympathectomy before cold exposure delayed the cold induced T3 elevation occurring during the first week of cold exposure (controls: from 1.16 +/- 0.19 to 1.44 +/- 0.29 nmol/l; sympathectomized rats: from 1.07 +/- 0.12 to 1.17 +/- 0.22 nmol/l). After 2 weeks of cold exposure the T3 levels of controls and sympathectomized rats were not significantly different (controls: 1.45 +/- 0.12 nmol/l, sympathectomized  rats: 1.38 +/- 0.15 nmol/l). No effect of sympathectomy was observed on T4 levels. These experiments show that the role of sympathetic activity in increasing T3 is not clear during cold exposure. They provide some evidence that sympathetic activity may play a role in the initiation of the process leading to increased T3 plasma levels during cold exposure."}
{"id": "PMID:474031", "title": "[Physiopathology of lymphedema (author's transl)].", "content": "On the vast and still unclear problem of lymphedema, the author stresses the major role played by the permeability of the blood vessel wall to proteins, with accumulation of these in the interstitial space. The walls of capillaries are not semi-permeable. There is a passage of proteins even under normal conditions. Increased during inflammatory process, it determines the accumulation of protein molecules in the interstitial space. The increased passage of protein into the interstitial space during a temporary process is often the causative factor in lymphedema. All situations which interfere with the drainage of these substances by the lymphatic system maintains the edema and leads to a passage to chronicity.", "contents": "[Physiopathology of lymphedema (author's transl)]. On the vast and still unclear problem of lymphedema, the author stresses the major role played by the permeability of the blood vessel wall to proteins, with accumulation of these in the interstitial space. The walls of capillaries are not semi-permeable. There is a passage of proteins even under normal conditions. Increased during inflammatory process, it determines the accumulation of protein molecules in the interstitial space. The increased passage of protein into the interstitial space during a temporary process is often the causative factor in lymphedema. All situations which interfere with the drainage of these substances by the lymphatic system maintains the edema and leads to a passage to chronicity."}
{"id": "PMID:474041", "title": "A pitfall in the ovarian ascorbic acid depletion assay (OAAD): effect of unilateral ovariectomy, hCG and asialo-hCG.", "content": "The estimation of the effect of desialylation on the biological activity of human chorionic gonadotrophin (hCG), as determined in the ovarian ascorbic acid depletion assay (OAAD), according to the modification in which the animals are used twice, appeared to be dependent on the assay conditions. Animals which received hCG in the first assay (OAAD-1) gave higher potency estimates for hCG and asialo-hCG in OAAD-1. The results of the experiments indicate that the ovarian sensitivity for hCG and asialo-hCG is increased after administration of hCG and unilateral ovariectomy and decreased after administration of asialo-hCG.", "contents": "A pitfall in the ovarian ascorbic acid depletion assay (OAAD): effect of unilateral ovariectomy, hCG and asialo-hCG. The estimation of the effect of desialylation on the biological activity of human chorionic gonadotrophin (hCG), as determined in the ovarian ascorbic acid depletion assay (OAAD), according to the modification in which the animals are used twice, appeared to be dependent on the assay conditions. Animals which received hCG in the first assay (OAAD-1) gave higher potency estimates for hCG and asialo-hCG in OAAD-1. The results of the experiments indicate that the ovarian sensitivity for hCG and asialo-hCG is increased after administration of hCG and unilateral ovariectomy and decreased after administration of asialo-hCG."}
{"id": "PMID:474032", "title": "[Experimental evidence for motoric rehabilitation in lymphoedema (author's transl)].", "content": "The authors distinguish three modalities of lymphtransport in lymphoedema. 1. Enhanced evacuation by existing lymphatics. 2. Enhanced evacuation by regenerated lymphatics. 3. Enhanced evacuation by lympho-lymphatic anastomoses. Experiments with 500 white mice indicate that the resorption of lymphoedema is increased by guided compression, although some manipulations must be performed with care, especially when regenerated lymphatics are present. The lymphatic flow in a limb is also enhanced by a special drainage technique at the proximal part of it. Likewise the lymph can be manipulated into the opposite limb by anastomotic channels after unilateral lymphadenectomy.", "contents": "[Experimental evidence for motoric rehabilitation in lymphoedema (author's transl)]. The authors distinguish three modalities of lymphtransport in lymphoedema. 1. Enhanced evacuation by existing lymphatics. 2. Enhanced evacuation by regenerated lymphatics. 3. Enhanced evacuation by lympho-lymphatic anastomoses. Experiments with 500 white mice indicate that the resorption of lymphoedema is increased by guided compression, although some manipulations must be performed with care, especially when regenerated lymphatics are present. The lymphatic flow in a limb is also enhanced by a special drainage technique at the proximal part of it. Likewise the lymph can be manipulated into the opposite limb by anastomotic channels after unilateral lymphadenectomy."}
{"id": "PMID:474030", "title": "Left chylothorax complicating a translumbar aortography.", "content": "The authors report a case of left chylothorax after a translumbar aortography, successfully managed by conservative means. As far as they known 16 similar cases are described. They review the literature on this subject.", "contents": "Left chylothorax complicating a translumbar aortography. The authors report a case of left chylothorax after a translumbar aortography, successfully managed by conservative means. As far as they known 16 similar cases are described. They review the literature on this subject."}
{"id": "PMID:474042", "title": "The effects of low doses of cyproterone acetate on some functions of the reproductive system in normal men.", "content": "One daily dose of either 5 mg or 10 mg cyproterone acetate (CA) was administered to 2 groups of 4 fertile men for 6 months. The medication was preceded by a 3 months placebo period and followed by a recovery phase of 5 to 8 months. During CA-treatment the sperm count/ml decreased and the percentage of abnormal spermatozoa increased slightly (0.991 less than P less than 0.05). Sperm penetration assessed by the Kremer test did not show any decrease during treatment. Serum levels of testosterone and FSH decreased, but those of LH remained unchanged during treatment. Two pregnancies occurred after 13/4 and 51/2 months of CA-treatment. The serum-CA concentration in these 2 volonteers did not differ from that of the remainder. Three subjects who began the study were withdrawn because of depressive mood changes (2) and weakness combined with dizziness (1). Data from these subjects were not included. The results indicate that daily doses of 5 mg and 10 mg of cyproterone acetate are not effective as a male contraceptive.", "contents": "The effects of low doses of cyproterone acetate on some functions of the reproductive system in normal men. One daily dose of either 5 mg or 10 mg cyproterone acetate (CA) was administered to 2 groups of 4 fertile men for 6 months. The medication was preceded by a 3 months placebo period and followed by a recovery phase of 5 to 8 months. During CA-treatment the sperm count/ml decreased and the percentage of abnormal spermatozoa increased slightly (0.991 less than P less than 0.05). Sperm penetration assessed by the Kremer test did not show any decrease during treatment. Serum levels of testosterone and FSH decreased, but those of LH remained unchanged during treatment. Two pregnancies occurred after 13/4 and 51/2 months of CA-treatment. The serum-CA concentration in these 2 volonteers did not differ from that of the remainder. Three subjects who began the study were withdrawn because of depressive mood changes (2) and weakness combined with dizziness (1). Data from these subjects were not included. The results indicate that daily doses of 5 mg and 10 mg of cyproterone acetate are not effective as a male contraceptive."}
{"id": "PMID:474043", "title": "Serum gastrin and serum calcitonin in patients with chronic renal failure.", "content": "The interrelationship between serum gastrin and serum calcitonin concentrations was studied in 73 patients with chronic renal failure. In both haemodialyzed and non-dialyzed patients increased serum concentrations of these hormones were found compared with normal controls. In non-dialyzed patients with creatinine clearance above 10 ml/min a highly significant correlation between serum gastrin and creatinine clearance was found, whereas no correlation was found in patients with creatinine clearance below 10 ml/min. Between serum gastrin and serum calcitonin, a significant positive correlation was found in non-dialyzed patients. These findings may be explained by a relationship between the two hormones or be secondary to a decreased elimination due to the reduced renal function.", "contents": "Serum gastrin and serum calcitonin in patients with chronic renal failure. The interrelationship between serum gastrin and serum calcitonin concentrations was studied in 73 patients with chronic renal failure. In both haemodialyzed and non-dialyzed patients increased serum concentrations of these hormones were found compared with normal controls. In non-dialyzed patients with creatinine clearance above 10 ml/min a highly significant correlation between serum gastrin and creatinine clearance was found, whereas no correlation was found in patients with creatinine clearance below 10 ml/min. Between serum gastrin and serum calcitonin, a significant positive correlation was found in non-dialyzed patients. These findings may be explained by a relationship between the two hormones or be secondary to a decreased elimination due to the reduced renal function."}
{"id": "PMID:474044", "title": "[Treatment of patients with hemophilia A having antibodies to factor VIII].", "content": "Four haemophiliacs with antibodies to factor VIII were treated on 7 occasions with fraction FEIBA. Satisfactory clinical results were achieved in patients receiving large doses of the preparation (63-105 units/kg). The administration of fraction FEIBA produced shortening of the whole blood clotting time but there was only a slight correction in the activated partial thromboplastin time. Two patients received prothrombin complex concentrate without clinical improvement in 1 case a good haemostatic effect was achieved after the infusion of cryoprecipitate and in 1 postoperative bleeding ceased with the administration of porcine factor VIII Immunosuppressive therapy with cyclophosphamide in 3 patients failed to prevent the anamnestic rise in antibody titer.", "contents": "[Treatment of patients with hemophilia A having antibodies to factor VIII]. Four haemophiliacs with antibodies to factor VIII were treated on 7 occasions with fraction FEIBA. Satisfactory clinical results were achieved in patients receiving large doses of the preparation (63-105 units/kg). The administration of fraction FEIBA produced shortening of the whole blood clotting time but there was only a slight correction in the activated partial thromboplastin time. Two patients received prothrombin complex concentrate without clinical improvement in 1 case a good haemostatic effect was achieved after the infusion of cryoprecipitate and in 1 postoperative bleeding ceased with the administration of porcine factor VIII Immunosuppressive therapy with cyclophosphamide in 3 patients failed to prevent the anamnestic rise in antibody titer."}
{"id": "PMID:474045", "title": "[Tooth extraction in patients suffering from hemophilia A under the cover of cryoprecipitates and epsilon-aminocaproic acid].", "content": "Tooth extractions were carried out in 75 patients with haemophilia A after infusion of a single dose of cryoprecipitate and 4 g of epsilon-aminocaproic acid. During the 7-10 day period of follow-up, the patients received epsilon-aminocaproic acid by mouth. In 32 of 44 patients with severe or moderate haemophilia and in 22 of 31 patients with mild haemophilia, healing was uneventful and no bleeding complications occurred. Only in 5 patients with severe haemophilia and in 1 patient with mild heamophilia bleeding from tooth sockets was extensive enough to require further replacement therapy. The results show that epsilon-aminocaproic acid in conjunction with a single infusion of cryoprecipitate can insure hemostasis after dental extraction in patients with haemophilia A.", "contents": "[Tooth extraction in patients suffering from hemophilia A under the cover of cryoprecipitates and epsilon-aminocaproic acid]. Tooth extractions were carried out in 75 patients with haemophilia A after infusion of a single dose of cryoprecipitate and 4 g of epsilon-aminocaproic acid. During the 7-10 day period of follow-up, the patients received epsilon-aminocaproic acid by mouth. In 32 of 44 patients with severe or moderate haemophilia and in 22 of 31 patients with mild haemophilia, healing was uneventful and no bleeding complications occurred. Only in 5 patients with severe haemophilia and in 1 patient with mild heamophilia bleeding from tooth sockets was extensive enough to require further replacement therapy. The results show that epsilon-aminocaproic acid in conjunction with a single infusion of cryoprecipitate can insure hemostasis after dental extraction in patients with haemophilia A."}
{"id": "PMID:474064", "title": "Comparative study of cardiovascular, neurological and metabolic side effects of 8 narcotics in dogs. Pethidine, piritramide, morphine, phenoperidine, fentanyl, R 39 209, sufentanil, R 34 995. II. Comparative study on the epileptoid activity of the narcotics used in high and massive doses in curarised and mechanically ventilated dogs.", "content": "The experimental design, described in part I, was again used here. The electrocortical activity was registered with an EEG amplifier using a bipolar derivation of needle electrodes fixed in the scalp of a dog in the fronto-occipital position. In this situation the convlusion threshold for the 8 substances is as follows: pethidine 20 mg.kg-1 I.V., piritramide 30, morphine 180, phenoperidine 4, R 39 209 5, fentanyl 4, sufentanil 4 and R 34 995 10 mg.kg-1 I.V. Comparing the I.V. doses producing severe convulsions with the doses necessary for deep surgical analgesia a safety margin of neurological toxicity was calculated. This was for pethidine 2.2, for piritramide 6.6, for phenoperidine 16, for R 39 209 62.5, for morphine 72, for fentanyl 160, for sufentanil 1 000 and for R 34 995 10 000. It is concluded that for pure narcotics there exists an inverse relationship between analgesic potency and neurological toxicity which is always accompanied by a hyperactivity of the automatic nervous system. Factors modifying the convulsive level of the narcotics are still under investigation. In the meanwhile it can be stated that the association of a strong narcotic with flunitrazepam, droperidol or etomidate will increase the convulsion threshold of the morphinomimetics.", "contents": "Comparative study of cardiovascular, neurological and metabolic side effects of 8 narcotics in dogs. Pethidine, piritramide, morphine, phenoperidine, fentanyl, R 39 209, sufentanil, R 34 995. II. Comparative study on the epileptoid activity of the narcotics used in high and massive doses in curarised and mechanically ventilated dogs. The experimental design, described in part I, was again used here. The electrocortical activity was registered with an EEG amplifier using a bipolar derivation of needle electrodes fixed in the scalp of a dog in the fronto-occipital position. In this situation the convlusion threshold for the 8 substances is as follows: pethidine 20 mg.kg-1 I.V., piritramide 30, morphine 180, phenoperidine 4, R 39 209 5, fentanyl 4, sufentanil 4 and R 34 995 10 mg.kg-1 I.V. Comparing the I.V. doses producing severe convulsions with the doses necessary for deep surgical analgesia a safety margin of neurological toxicity was calculated. This was for pethidine 2.2, for piritramide 6.6, for phenoperidine 16, for R 39 209 62.5, for morphine 72, for fentanyl 160, for sufentanil 1 000 and for R 34 995 10 000. It is concluded that for pure narcotics there exists an inverse relationship between analgesic potency and neurological toxicity which is always accompanied by a hyperactivity of the automatic nervous system. Factors modifying the convulsive level of the narcotics are still under investigation. In the meanwhile it can be stated that the association of a strong narcotic with flunitrazepam, droperidol or etomidate will increase the convulsion threshold of the morphinomimetics."}
{"id": "PMID:474065", "title": "EEG and cognitive impairment in presenile dementia.", "content": "EEG and psychometric findings were studied in a group of 57 patients consisting of 19 cases of Alzheimer's disease, 7 cases of Pick's disease, 24 cases of cerebrovascular dementia (CVD) and a group of 7 cases with dementia of various other etiology. The diagnoses have so far been confirmed by autopsy in 23 out of 57 cases. EEG was evaluated by means of visual inspection. Psychometric studies enabled a classification into 5 psychometric defect groups according to the degree of dementia. An overall good correlation was found between the degree of dementia and EEG abnormality. A significant correlation between the test socre and the EEG was found only for the vocabulary test and paired associates test. However, on the reaction time test, color word test, and Koh's block design test, large patient groups were untestable, and a highly significant correlation was found between non-testability and severely abnormal EEG. The Alzheimer and the CVD groups differed distinctly, most of the Alzheimer cases showing a severe or moderate degree of EEG abnormality and dementia, whereas in the CVD cases, the dementia was less pronounced and the EEG often normal or only slightly abnormal. Four out of seven cases of Pick's disease had a normal EEG, which distinguished them from the Alzheimer cases which had a comparable psychometric defect.", "contents": "EEG and cognitive impairment in presenile dementia. EEG and psychometric findings were studied in a group of 57 patients consisting of 19 cases of Alzheimer's disease, 7 cases of Pick's disease, 24 cases of cerebrovascular dementia (CVD) and a group of 7 cases with dementia of various other etiology. The diagnoses have so far been confirmed by autopsy in 23 out of 57 cases. EEG was evaluated by means of visual inspection. Psychometric studies enabled a classification into 5 psychometric defect groups according to the degree of dementia. An overall good correlation was found between the degree of dementia and EEG abnormality. A significant correlation between the test socre and the EEG was found only for the vocabulary test and paired associates test. However, on the reaction time test, color word test, and Koh's block design test, large patient groups were untestable, and a highly significant correlation was found between non-testability and severely abnormal EEG. The Alzheimer and the CVD groups differed distinctly, most of the Alzheimer cases showing a severe or moderate degree of EEG abnormality and dementia, whereas in the CVD cases, the dementia was less pronounced and the EEG often normal or only slightly abnormal. Four out of seven cases of Pick's disease had a normal EEG, which distinguished them from the Alzheimer cases which had a comparable psychometric defect."}
{"id": "PMID:474066", "title": "Prenatal exposure to methadone HCL in relationship to body and brain growth in the rat.", "content": "Offspring from methadone-treated Wistar rats (last trimester of pregnancy), as compared to ad lib and pair fed controls, showed a reduction of body growth which continued throughout the 28-day period during which animals were observed after birth. Brain growth, as indicated by weight, cortical thickness and number of cells in the neocortex also showed a reduction in growth, which was apparent only during the first 14 days, after which there were no differences between the groups. However, in the hippocampus, neuronal density changes/unit area continued throughout the 28-day period in the methadone exposed group and to a lesser extent in the PF group suggesting that this area of brain did not return to normal dimensions or degree of maturation by the end of the third week. Since it has been suggested that any deficit in growth associated with prenatalmethadone-exposure might be due to a reduction in food intake, a group pair-fed to the amount of food consumed by the methadone group was included in the study. Generally, the data from the PF group was intermediate between that obtained from the ad lib fed controls and the methadone-exposed animals. Thus, while some of the growth reduction observed in the methadone-exposed pups could have been due to the impaired nutritional status of their mothers during the last third of pregnancy, a part of the reduced growth must be attributed to the drug treatment, since the reduced growth exceeded that observed in the pair-fed group.", "contents": "Prenatal exposure to methadone HCL in relationship to body and brain growth in the rat. Offspring from methadone-treated Wistar rats (last trimester of pregnancy), as compared to ad lib and pair fed controls, showed a reduction of body growth which continued throughout the 28-day period during which animals were observed after birth. Brain growth, as indicated by weight, cortical thickness and number of cells in the neocortex also showed a reduction in growth, which was apparent only during the first 14 days, after which there were no differences between the groups. However, in the hippocampus, neuronal density changes/unit area continued throughout the 28-day period in the methadone exposed group and to a lesser extent in the PF group suggesting that this area of brain did not return to normal dimensions or degree of maturation by the end of the third week. Since it has been suggested that any deficit in growth associated with prenatalmethadone-exposure might be due to a reduction in food intake, a group pair-fed to the amount of food consumed by the methadone group was included in the study. Generally, the data from the PF group was intermediate between that obtained from the ad lib fed controls and the methadone-exposed animals. Thus, while some of the growth reduction observed in the methadone-exposed pups could have been due to the impaired nutritional status of their mothers during the last third of pregnancy, a part of the reduced growth must be attributed to the drug treatment, since the reduced growth exceeded that observed in the pair-fed group."}
{"id": "PMID:474067", "title": "Erythrocyte spectrofluorometric abnormalities in Duchenne patients and carriers. A new approach to carrier detection.", "content": "Duchenne muscular dystrophy (DMD) is a disorder whose pathogenesis is attributed, according to the most recent theories, to generalized membrane abnormalities, including red cell membrane. The study of erythrocyte ghosts in patients affected by DMD showed alterations in ion transport and in various enzymatic activities. Employing a spectrofluorometric method using 1-anilino-8-naphthalene sufonate (1.8-ANS) we have tried to characterize the erythrocyte ghosts of DMD patients, definite DMD carriers and normal controls, matched for age and sex. We found an increase of membrane polarity in DMD and definite DMD-carrier erythrocyte ghosts. Using this new method we were able to: - confirm by means of a different technique the previous observations of many authors on erythrocyte ghost alterations in DMD - find a new technique useful in detecting the DMD carriers. It is noteworthy that in such a way the detection rate of definite DMD carriers was higher than with the CPK test.", "contents": "Erythrocyte spectrofluorometric abnormalities in Duchenne patients and carriers. A new approach to carrier detection. Duchenne muscular dystrophy (DMD) is a disorder whose pathogenesis is attributed, according to the most recent theories, to generalized membrane abnormalities, including red cell membrane. The study of erythrocyte ghosts in patients affected by DMD showed alterations in ion transport and in various enzymatic activities. Employing a spectrofluorometric method using 1-anilino-8-naphthalene sufonate (1.8-ANS) we have tried to characterize the erythrocyte ghosts of DMD patients, definite DMD carriers and normal controls, matched for age and sex. We found an increase of membrane polarity in DMD and definite DMD-carrier erythrocyte ghosts. Using this new method we were able to: - confirm by means of a different technique the previous observations of many authors on erythrocyte ghost alterations in DMD - find a new technique useful in detecting the DMD carriers. It is noteworthy that in such a way the detection rate of definite DMD carriers was higher than with the CPK test."}
{"id": "PMID:474068", "title": "Alcoholism and cerebrovascular thrombosis in the young.", "content": "The etiology of cerebrovascular thrombosis in normotensives has not been elucidated. This controlled retrospective study examines the association between alcoholism and cerebrovascular thrombosis in patients under age 50. The hospital records of 49 patients under age 50 with a diagnosis of cerebrovascular thrombosis without hypertension were reviewed for possible predisposing factors. Alcohol abuse is the one common denominator among the males in the study.", "contents": "Alcoholism and cerebrovascular thrombosis in the young. The etiology of cerebrovascular thrombosis in normotensives has not been elucidated. This controlled retrospective study examines the association between alcoholism and cerebrovascular thrombosis in patients under age 50. The hospital records of 49 patients under age 50 with a diagnosis of cerebrovascular thrombosis without hypertension were reviewed for possible predisposing factors. Alcohol abuse is the one common denominator among the males in the study."}
{"id": "PMID:474069", "title": "Brain proteins in experimental portal-systemic shunting.", "content": "Experimental portal-systemic shunting is accompanied by a reduction of the soluble brain proteins. Isoelectric focusing does not indicate any selective decrease of one particular protein or group of proteins. The reduction persists with sustained shunting. The interference with brain protein metabolism is compatible with a grossly normal behavior, alterness and locomotion.", "contents": "Brain proteins in experimental portal-systemic shunting. Experimental portal-systemic shunting is accompanied by a reduction of the soluble brain proteins. Isoelectric focusing does not indicate any selective decrease of one particular protein or group of proteins. The reduction persists with sustained shunting. The interference with brain protein metabolism is compatible with a grossly normal behavior, alterness and locomotion."}
{"id": "PMID:474070", "title": "New ultrastructural evidence for a protein transport system in endothelial cells of gerbil brains.", "content": "Pathways for transfer of horseradish peroxidase (HRP) across the cerebral microvasculature were studied in Mongolian gerbils after inducing either unilateral carotid-artery ligation or intracarotid air embolism. Electron microscopy on samples from both ipsilateral and contralateral brain hemispheres showed the reaction product (HRP) in the endothelial cytoplasm of capillaries and arteriols in all animals; in the basal lamina, HRP was seen only after long (3--4 h) ischemic periods. HRP was seen within both spherical and tubular structures, with the latter appearing in obliquely or longitudinally sectioned blood vessels. These endothelial channel-like structures are part of a tubulovesicular network which seemingly exists in the normal endothelium of the gerbil brain. The tubules serve as pathways for proteins into the brain; protein transfer becomes enhanced following circulatory injuries such as ischemia.", "contents": "New ultrastructural evidence for a protein transport system in endothelial cells of gerbil brains. Pathways for transfer of horseradish peroxidase (HRP) across the cerebral microvasculature were studied in Mongolian gerbils after inducing either unilateral carotid-artery ligation or intracarotid air embolism. Electron microscopy on samples from both ipsilateral and contralateral brain hemispheres showed the reaction product (HRP) in the endothelial cytoplasm of capillaries and arteriols in all animals; in the basal lamina, HRP was seen only after long (3--4 h) ischemic periods. HRP was seen within both spherical and tubular structures, with the latter appearing in obliquely or longitudinally sectioned blood vessels. These endothelial channel-like structures are part of a tubulovesicular network which seemingly exists in the normal endothelium of the gerbil brain. The tubules serve as pathways for proteins into the brain; protein transfer becomes enhanced following circulatory injuries such as ischemia."}
{"id": "PMID:474071", "title": "Chronic relapsing experimental allergic encephalomyelitis: effect of age at the time of sensitization on clinical course and pathology.", "content": "Experimental allergic encephalomyelitis was induced by sensitization with guinea pig spinal cord in complete Freund's adjuvant with increased content of myobacterium in Hartley guinea pigs between the first and 28th day after birth. The animals either died during acute EAE or showed chronic progressive, chronic relapsing or chronic EAE with delayed onset. In animals immunized between the 1 and 13 day after birth, predominatly ordinary and chronic progressive EAE was noted, whereas animals sensitized between the 14th and 23rd day frequently suffered from hyperacute or chronic relapsing EAE. Immunization on the 24th day led to very high mortality in acute EAE. The relation of the pathohistologic alterations to different types of human inflammatory demyelinative disease is discussed.", "contents": "Chronic relapsing experimental allergic encephalomyelitis: effect of age at the time of sensitization on clinical course and pathology. Experimental allergic encephalomyelitis was induced by sensitization with guinea pig spinal cord in complete Freund's adjuvant with increased content of myobacterium in Hartley guinea pigs between the first and 28th day after birth. The animals either died during acute EAE or showed chronic progressive, chronic relapsing or chronic EAE with delayed onset. In animals immunized between the 1 and 13 day after birth, predominatly ordinary and chronic progressive EAE was noted, whereas animals sensitized between the 14th and 23rd day frequently suffered from hyperacute or chronic relapsing EAE. Immunization on the 24th day led to very high mortality in acute EAE. The relation of the pathohistologic alterations to different types of human inflammatory demyelinative disease is discussed."}
{"id": "PMID:474072", "title": "A sequential study of changes in the brain and cerebrospinal fluid of the rat following triethyltin poisoning.", "content": "Following a single intravenous (i.v.) injection of triethyltin (10 mg/kg) in rats, vacuoles appeared in the myelin sheath within 3 h and they progressively increased in size between 6 and 24 h. Their development was closely correlated with a progressive increase in water, sodium, and chloride content of the brain, and of cerebrospinal fluid (CSF) pressure. Oedema was more extensive in tissues consisting predominantly of white matter rather than grey matter. The sustained increase in CSF pressure did not precede the development of lesions and was the result of, rather than the cause of, brain oedema and swelling. These findings indicate that triethyltin has a direct effect on the myelin sheath.", "contents": "A sequential study of changes in the brain and cerebrospinal fluid of the rat following triethyltin poisoning. Following a single intravenous (i.v.) injection of triethyltin (10 mg/kg) in rats, vacuoles appeared in the myelin sheath within 3 h and they progressively increased in size between 6 and 24 h. Their development was closely correlated with a progressive increase in water, sodium, and chloride content of the brain, and of cerebrospinal fluid (CSF) pressure. Oedema was more extensive in tissues consisting predominantly of white matter rather than grey matter. The sustained increase in CSF pressure did not precede the development of lesions and was the result of, rather than the cause of, brain oedema and swelling. These findings indicate that triethyltin has a direct effect on the myelin sheath."}
{"id": "PMID:474073", "title": "Postnatal vascular growth in the neocortex of normal and protein-deprived rats. Morphometric studies.", "content": "The postnatal vascular growth in the neocortical area 18 of normal and pre- and postnatally protein-deprived rats was examined. For control rats the specific length, the specific surface and the volume fraction of vessels increased rapidly between 7 and 20 days of age. Thereafter, only a minor increase was seen. In protein-deprived rats there was no increase in the specific length of vessels between 7 and 10 days of age and this variable was still reduced at 30 days of age compared to controls. This reduction was due to a decrease in the specific length of thin vessels (luminal diameter less than 8.25 mu) whereas the specific length of wider vessels was not affected by the protein deprivation. There were no significant differences in the specific surface or volume fraction of vessels between control and protein-deprived rats. These findings indicate an adaptive increase in luminal diameter of vessels in the protein deprived rats during postnatal development. At 90 days of age no significant differences between vascular variables of control and protein-deprived rats were seen.", "contents": "Postnatal vascular growth in the neocortex of normal and protein-deprived rats. Morphometric studies. The postnatal vascular growth in the neocortical area 18 of normal and pre- and postnatally protein-deprived rats was examined. For control rats the specific length, the specific surface and the volume fraction of vessels increased rapidly between 7 and 20 days of age. Thereafter, only a minor increase was seen. In protein-deprived rats there was no increase in the specific length of vessels between 7 and 10 days of age and this variable was still reduced at 30 days of age compared to controls. This reduction was due to a decrease in the specific length of thin vessels (luminal diameter less than 8.25 mu) whereas the specific length of wider vessels was not affected by the protein deprivation. There were no significant differences in the specific surface or volume fraction of vessels between control and protein-deprived rats. These findings indicate an adaptive increase in luminal diameter of vessels in the protein deprived rats during postnatal development. At 90 days of age no significant differences between vascular variables of control and protein-deprived rats were seen."}
{"id": "PMID:474074", "title": "Postnatal vascular growth in the cerebellar cortex of normal and protein-deprived rats. Morphometric studies.", "content": "The postnatal vascular growth in the cortex of vermis cerebelli folium IX of normal and pre- and postnatally protein-deprived rats was examined. The rate of increase in specific length of vessels seem to parallel the functional maturation of neurons in all cortical layers. From the first postnatal week there is a higher specific length of vessels in the Purkinje cell layer than in the adjoining parts of the molecular and granular layers. The results indicate that such differences are present also after the period of rapid vascular growth. Protein deprivation appears to affect the postnatal increase in specific length of vessels less in the Purkinje cell layer than in the granular and molecular layer where a significant reduction compared to controls was seen for the interval 7--20 days of age. At 90 days of age no significant differences were seen between control and protein-deprived rats.", "contents": "Postnatal vascular growth in the cerebellar cortex of normal and protein-deprived rats. Morphometric studies. The postnatal vascular growth in the cortex of vermis cerebelli folium IX of normal and pre- and postnatally protein-deprived rats was examined. The rate of increase in specific length of vessels seem to parallel the functional maturation of neurons in all cortical layers. From the first postnatal week there is a higher specific length of vessels in the Purkinje cell layer than in the adjoining parts of the molecular and granular layers. The results indicate that such differences are present also after the period of rapid vascular growth. Protein deprivation appears to affect the postnatal increase in specific length of vessels less in the Purkinje cell layer than in the granular and molecular layer where a significant reduction compared to controls was seen for the interval 7--20 days of age. At 90 days of age no significant differences were seen between control and protein-deprived rats."}
{"id": "PMID:474075", "title": "Fetal globoid cell leukodystrophy in one of twins.", "content": "Twins with high-risk globoid cell leukodystrophy (GLD) at the 6th gestational month were studied morphologically and enzymatically. One of them was affected and the other was probably the carrier of this disease. Central nervous system and other viscera developed normally for the gestational age in both fetuses. The lesions of GLD were found in the fetus with marked deficiency of galactocerebrosidase, but not in the probable carrier. The lesions were located only in the considerably myelinated areas viz., the brain stem and the spinal cord. Ultrastructurally, the globoid cells in the fetal GLD seemed to originate from glial cells, probably oligodendroglia.", "contents": "Fetal globoid cell leukodystrophy in one of twins. Twins with high-risk globoid cell leukodystrophy (GLD) at the 6th gestational month were studied morphologically and enzymatically. One of them was affected and the other was probably the carrier of this disease. Central nervous system and other viscera developed normally for the gestational age in both fetuses. The lesions of GLD were found in the fetus with marked deficiency of galactocerebrosidase, but not in the probable carrier. The lesions were located only in the considerably myelinated areas viz., the brain stem and the spinal cord. Ultrastructurally, the globoid cells in the fetal GLD seemed to originate from glial cells, probably oligodendroglia."}
{"id": "PMID:474076", "title": "Intramedullary ependymoma producing collagen. A clinical and pathological study.", "content": "The authors describe the clinical and pathological observations of a thoracolumbar intramedullary ependymoma. A voluminous conus of collagenous tissue was located in the center of the tumor. This most unusual histological finding is compared to rare cases of the literature describing the presence of bone and cartilage in ependymomas.", "contents": "Intramedullary ependymoma producing collagen. A clinical and pathological study. The authors describe the clinical and pathological observations of a thoracolumbar intramedullary ependymoma. A voluminous conus of collagenous tissue was located in the center of the tumor. This most unusual histological finding is compared to rare cases of the literature describing the presence of bone and cartilage in ependymomas."}
{"id": "PMID:474077", "title": "A Golgi and electron microscopic study of a dysplastic gangliocytoma of the cerebellum.", "content": "The fine structure of a dysplastic gangliocytoma of the cerebellum is studied by means of the Golgi method and electron microscopic examination. Thick proximally unbranched dendrites with terminal arborizations and varicose inflorescences in the form of a basket are stained with the Golgi method. Axons are always descendant to the inner myelinated layer of the redistributed cerebellar cortex, while ascendant collaterals are observed at the level of the outer myelinated layer. Clear and dense-core vesicles and synapses are common in the cellular profiles under electron microscopic examination. From these data and because of the lack of putative connections through the white matter, an organized, self-regulated, catecholamine-mediated complex may be postulated.", "contents": "A Golgi and electron microscopic study of a dysplastic gangliocytoma of the cerebellum. The fine structure of a dysplastic gangliocytoma of the cerebellum is studied by means of the Golgi method and electron microscopic examination. Thick proximally unbranched dendrites with terminal arborizations and varicose inflorescences in the form of a basket are stained with the Golgi method. Axons are always descendant to the inner myelinated layer of the redistributed cerebellar cortex, while ascendant collaterals are observed at the level of the outer myelinated layer. Clear and dense-core vesicles and synapses are common in the cellular profiles under electron microscopic examination. From these data and because of the lack of putative connections through the white matter, an organized, self-regulated, catecholamine-mediated complex may be postulated."}
{"id": "PMID:474078", "title": "The effects of ageing on the pigmented nerve cells of the human locus caeruleous and substantia nigra.", "content": "The nucleolar volume and melanin content of the human locus caeruleus and substantia nigra has been measured in 70 persons of age range new born to 91 years, all of whom were at death free from overt neurological illness. Both cell types show a reduction in nucleolar size with advancing age, which becomes disproportionately larger towards old age. At 90 years of age, the change in nucleolar volume in cells of the locus caeruleus amounts to about 5% whereas cells of the substantia nigra show a loss of 20%. The greater decrease in nucleolar volume in cells of the substantia nigra is attributed to the higher concentration of melanin pigment occurring in these cells at old age, rather than the absolute amount present. This marked decline in nucleolar volume in cells of substantia nigra indicates a reduced activity in the cell which, in turn, may be reflected in the difficulty in control and coordination in muscular activity commonly seen in normal elderly persons.", "contents": "The effects of ageing on the pigmented nerve cells of the human locus caeruleous and substantia nigra. The nucleolar volume and melanin content of the human locus caeruleus and substantia nigra has been measured in 70 persons of age range new born to 91 years, all of whom were at death free from overt neurological illness. Both cell types show a reduction in nucleolar size with advancing age, which becomes disproportionately larger towards old age. At 90 years of age, the change in nucleolar volume in cells of the locus caeruleus amounts to about 5% whereas cells of the substantia nigra show a loss of 20%. The greater decrease in nucleolar volume in cells of the substantia nigra is attributed to the higher concentration of melanin pigment occurring in these cells at old age, rather than the absolute amount present. This marked decline in nucleolar volume in cells of substantia nigra indicates a reduced activity in the cell which, in turn, may be reflected in the difficulty in control and coordination in muscular activity commonly seen in normal elderly persons."}
{"id": "PMID:474079", "title": "Ultrasonographic study on changes in axial eye dimensions after encircling procedure in retinal detachment surgery.", "content": "The pre- and postoperative results of ultrasonographic measurements on the axial ocular components in 10 phakic eyes with retinal detachment treated with encircling silicone rubber band are presented. A significant increase (P less than 0.001) in axial eye length from 0.62 to 1.24 mm (average: 0.98 mm) was found. The elongation of the eye was caused by a corresponding increase in the length of the vitreous cavity. No significant changes were found in the anterior segment of the eyes. These data demonstrate that the postoperative refractive change in a myopic direction, which an encircling procedure with moderate indentation often produces, is caused by an axial elongation of the eye.", "contents": "Ultrasonographic study on changes in axial eye dimensions after encircling procedure in retinal detachment surgery. The pre- and postoperative results of ultrasonographic measurements on the axial ocular components in 10 phakic eyes with retinal detachment treated with encircling silicone rubber band are presented. A significant increase (P less than 0.001) in axial eye length from 0.62 to 1.24 mm (average: 0.98 mm) was found. The elongation of the eye was caused by a corresponding increase in the length of the vitreous cavity. No significant changes were found in the anterior segment of the eyes. These data demonstrate that the postoperative refractive change in a myopic direction, which an encircling procedure with moderate indentation often produces, is caused by an axial elongation of the eye."}
{"id": "PMID:474080", "title": "Retinoblastoma in Sweden 1958--1971. A clinical and histopathological study.", "content": "This study includes all cases of retinoblastoma reported in Sweden between 1958 and 1971. The incidence of the disease was 1 per 18 000 live births. Only in six cases was there a familial history and five of these cases were bilateral. The tumour was bilateral in 37.5% of all cases. All cases with unilateral tumour had been treated with enucleation. In the bilateral cases one eye had also been enucleated and the other eye treated by local irradiation therapy. Tumour invasion into choroid was found in 29% and into the optic nerve on 11% of the cases. The mortality was only 4.5%.", "contents": "Retinoblastoma in Sweden 1958--1971. A clinical and histopathological study. This study includes all cases of retinoblastoma reported in Sweden between 1958 and 1971. The incidence of the disease was 1 per 18 000 live births. Only in six cases was there a familial history and five of these cases were bilateral. The tumour was bilateral in 37.5% of all cases. All cases with unilateral tumour had been treated with enucleation. In the bilateral cases one eye had also been enucleated and the other eye treated by local irradiation therapy. Tumour invasion into choroid was found in 29% and into the optic nerve on 11% of the cases. The mortality was only 4.5%."}
{"id": "PMID:474081", "title": "Asymmetric retinitis pigmentosa, luetic retinopathy and the question of unilateral retinitis pigmentosa.", "content": "A seropositive patient with the fundus appearance of monocular luetic retinopathy is presented. Electrophysiologically, this patient shows a late-onset form of progressive tapetoretinal degeneration (retinitis pigmentosa) presenting in an extremely asymmetric manner. The differential diagnosis of luetic and other inflammatory retinopathies vs. tapetoretinal degenerations is briefly discussed. Features distinguishing unilateral retinitis pigmentosa from bilateral tapetoretinal degenerations are reviewed, and the existence of unilateral retinitis pigmentosa as a genetic entity is questioned. Prolongation of ERG- a- and b-wave implicit times is characteristic of the tapetoretinal degenerations, and normal implicit times in the fellow eye are suggested as an additional criterion for the diagnosis of unilateral retinitis pigmentosa.", "contents": "Asymmetric retinitis pigmentosa, luetic retinopathy and the question of unilateral retinitis pigmentosa. A seropositive patient with the fundus appearance of monocular luetic retinopathy is presented. Electrophysiologically, this patient shows a late-onset form of progressive tapetoretinal degeneration (retinitis pigmentosa) presenting in an extremely asymmetric manner. The differential diagnosis of luetic and other inflammatory retinopathies vs. tapetoretinal degenerations is briefly discussed. Features distinguishing unilateral retinitis pigmentosa from bilateral tapetoretinal degenerations are reviewed, and the existence of unilateral retinitis pigmentosa as a genetic entity is questioned. Prolongation of ERG- a- and b-wave implicit times is characteristic of the tapetoretinal degenerations, and normal implicit times in the fellow eye are suggested as an additional criterion for the diagnosis of unilateral retinitis pigmentosa."}
{"id": "PMID:474082", "title": "Intramuscular iron therapy and tapetoretinal degeneration. A case report.", "content": "A 63-years-old male with pernicious anemia had been treated for 20 years with weekly injections of iron-dextran and cyanocobalamine. Ophthalmological examination revealed the ophthalmoscopic picture of a tapetoretinal degeneration, reduced visual acuity and narrow visual fields. ERG and dark-adaptation test were normal. Hematological examination including liver and bone marrow biopsies gave no support for the existence of systemic siderosis. It is proposed that the retinal degeneration is due to the extensive parenteral iron treatment with a total dose of approximately 100 grams of iron. This theory is supported by a previous experimental report.", "contents": "Intramuscular iron therapy and tapetoretinal degeneration. A case report. A 63-years-old male with pernicious anemia had been treated for 20 years with weekly injections of iron-dextran and cyanocobalamine. Ophthalmological examination revealed the ophthalmoscopic picture of a tapetoretinal degeneration, reduced visual acuity and narrow visual fields. ERG and dark-adaptation test were normal. Hematological examination including liver and bone marrow biopsies gave no support for the existence of systemic siderosis. It is proposed that the retinal degeneration is due to the extensive parenteral iron treatment with a total dose of approximately 100 grams of iron. This theory is supported by a previous experimental report."}
{"id": "PMID:474083", "title": "Unusual ocular manifestation in temporal arteritis. A case report.", "content": "Transitory oedema of the left orbit and maxillary sinus was revealed radiographically in an active phase of temporal arteritis. This is possibly caused by inflammatory changes of the soft tissues in these regions. Early signs of compromised retinal flow and anterior ischemic optic neuropathy were demonstrated with fluorescein angiography. This finding promoted early, immediate therapy.", "contents": "Unusual ocular manifestation in temporal arteritis. A case report. Transitory oedema of the left orbit and maxillary sinus was revealed radiographically in an active phase of temporal arteritis. This is possibly caused by inflammatory changes of the soft tissues in these regions. Early signs of compromised retinal flow and anterior ischemic optic neuropathy were demonstrated with fluorescein angiography. This finding promoted early, immediate therapy."}
{"id": "PMID:474084", "title": "Stimulus alternation and fast retinal potentials: photopic anc scotopic contributions.", "content": "Human retinal oscillatory potentials in response to an alternating checker-board pattern stimulus were studied in two subjects over an intensity range of 7.5 log units. Under scotopic conditions, two wavelets (S1 and S2) could be recorded. At an intensity of 1.9 photopic log Td four high frequency oscillations (O1--O4) were noticed and a discontinuity was observed in the corresponding luminance curve of the b-wave together with a sudden decrease in the magnitude of the standard deviation of the amplitude measures. The oscillations were noticed only on the ascending slope of the b-wave. With increasing stimulus intensity, their latency decreased at a slower rate than that of the b-wave and their number decreased. Each wavelet had an amplitude maximum at a certain stimulus intensity level. It was suggested that O1--O4 were generated by the activation of the photopic system and that S1 and S2 were of scotopic origin.", "contents": "Stimulus alternation and fast retinal potentials: photopic anc scotopic contributions. Human retinal oscillatory potentials in response to an alternating checker-board pattern stimulus were studied in two subjects over an intensity range of 7.5 log units. Under scotopic conditions, two wavelets (S1 and S2) could be recorded. At an intensity of 1.9 photopic log Td four high frequency oscillations (O1--O4) were noticed and a discontinuity was observed in the corresponding luminance curve of the b-wave together with a sudden decrease in the magnitude of the standard deviation of the amplitude measures. The oscillations were noticed only on the ascending slope of the b-wave. With increasing stimulus intensity, their latency decreased at a slower rate than that of the b-wave and their number decreased. Each wavelet had an amplitude maximum at a certain stimulus intensity level. It was suggested that O1--O4 were generated by the activation of the photopic system and that S1 and S2 were of scotopic origin."}
{"id": "PMID:474085", "title": "Familial cerebro-macular degeneration (the Stengel-Batten-Mayou-Spielmeyer-Vogt-Stock disease). Evaluation of the photoreceptors.", "content": "In a material comprising seven patients with familial cerebro-macular degeneration a grave visual disability had developed within a few years after the onset of the disease, this being at 3 1/2 to 7 years of age. The night vision was only moderately reduced. A severe red-green colour vision defect was demonstrated in three patients. This is in accord with the loss of red and green cone responses found in one patient by chromatic adaptation studies. On the other hand, a remarkably good response of the blue cone system was registered. Normal pulse amplitudes were found by dynamic tonometry in four patients indicating good choroidal circulation. This supports the theory that the degeneration of the neuroepithelium is of primary type. The selectivity in loss of response functions being demonstrated here, might, however, also be related to parallel degenerations taking place in ganglion cells.", "contents": "Familial cerebro-macular degeneration (the Stengel-Batten-Mayou-Spielmeyer-Vogt-Stock disease). Evaluation of the photoreceptors. In a material comprising seven patients with familial cerebro-macular degeneration a grave visual disability had developed within a few years after the onset of the disease, this being at 3 1/2 to 7 years of age. The night vision was only moderately reduced. A severe red-green colour vision defect was demonstrated in three patients. This is in accord with the loss of red and green cone responses found in one patient by chromatic adaptation studies. On the other hand, a remarkably good response of the blue cone system was registered. Normal pulse amplitudes were found by dynamic tonometry in four patients indicating good choroidal circulation. This supports the theory that the degeneration of the neuroepithelium is of primary type. The selectivity in loss of response functions being demonstrated here, might, however, also be related to parallel degenerations taking place in ganglion cells."}
{"id": "PMID:474086", "title": "Surgical stimulation of the uveoscleral outflow. Experimental studies on enucleated human eyes.", "content": "Six series of experiments were performed on 45 isolated cadaver eyes. In the first series, the outflow facility of the human sclera was measured, in the others, five surgical techniques for stimulating the uveo-scleral outflow were evaluted with the help of a constant pressure perfusion technique. It was found that the scleral C-value was pressure independent and its average value was equal to 0.0036 mm3/min/mmHg per 1 cm2 (100 mm2) of scleral surface. After the implantation of a scleral strip into the supra-uveal space, the mean increase in the total C-value was 23% of its initial value, ranging from 7 to 35%. After cyclodialysis the C-value increment varied from 17% to 66%, averaging 30%. The average increase in the outflow facility amounted to 60% (range 24--96%) in those cases where implantation of the scleral strip in the suprauveal space was combined with cyclodialysis. After implantation of a scleral strip, combined with subscleral sinusotomy, the C-value increment varied from 18 to 71%, averaging 41%. After cyclodialysis combined with goniospasis, the mean increase in the outflow facility coefficient was 62% (range 43%--108%).", "contents": "Surgical stimulation of the uveoscleral outflow. Experimental studies on enucleated human eyes. Six series of experiments were performed on 45 isolated cadaver eyes. In the first series, the outflow facility of the human sclera was measured, in the others, five surgical techniques for stimulating the uveo-scleral outflow were evaluted with the help of a constant pressure perfusion technique. It was found that the scleral C-value was pressure independent and its average value was equal to 0.0036 mm3/min/mmHg per 1 cm2 (100 mm2) of scleral surface. After the implantation of a scleral strip into the supra-uveal space, the mean increase in the total C-value was 23% of its initial value, ranging from 7 to 35%. After cyclodialysis the C-value increment varied from 17% to 66%, averaging 30%. The average increase in the outflow facility amounted to 60% (range 24--96%) in those cases where implantation of the scleral strip in the suprauveal space was combined with cyclodialysis. After implantation of a scleral strip, combined with subscleral sinusotomy, the C-value increment varied from 18 to 71%, averaging 41%. After cyclodialysis combined with goniospasis, the mean increase in the outflow facility coefficient was 62% (range 43%--108%)."}
{"id": "PMID:474087", "title": "Anterior chamber haemorrhage in the newborn after spontaneous delivery. A case report.", "content": "A case report is presented of anterior chamber haemorrhage occurring in one eye in a newborn after spontaneous delivery. At the age of two weeks the anterior chamber was clear but the vitreous cloudy. At the age of five weeks the vitreous had also cleared. The infant's later development was normal and there were no disorders in the function of the eye.", "contents": "Anterior chamber haemorrhage in the newborn after spontaneous delivery. A case report. A case report is presented of anterior chamber haemorrhage occurring in one eye in a newborn after spontaneous delivery. At the age of two weeks the anterior chamber was clear but the vitreous cloudy. At the age of five weeks the vitreous had also cleared. The infant's later development was normal and there were no disorders in the function of the eye."}
{"id": "PMID:474088", "title": "Fibrinolysis and traumatic hyphaema.", "content": "Since January 1st 1975, 310 patients with traumatic hyphaema have been treated with the antifibrinolytic drug tranexamic acid. One secondary haemorrhage has occurred, corresponding to a frequency of secondary haemorrhage of 0.32%. Eighty-five of these patients were treated as out-patients. In four patients with traumatic hyphaema, who were not treated with tranexamic acid, the serum content of activator inhibitor was determined daily. An increase was seen during the first five days after the trauma, followed by a marked fall on the 6th day. In the same four patients, the central corneal thickness was followed by daily measurements and compared to the variation in activator inhibitor.", "contents": "Fibrinolysis and traumatic hyphaema. Since January 1st 1975, 310 patients with traumatic hyphaema have been treated with the antifibrinolytic drug tranexamic acid. One secondary haemorrhage has occurred, corresponding to a frequency of secondary haemorrhage of 0.32%. Eighty-five of these patients were treated as out-patients. In four patients with traumatic hyphaema, who were not treated with tranexamic acid, the serum content of activator inhibitor was determined daily. An increase was seen during the first five days after the trauma, followed by a marked fall on the 6th day. In the same four patients, the central corneal thickness was followed by daily measurements and compared to the variation in activator inhibitor."}
{"id": "PMID:474089", "title": "Serum and aqueous humour concentration of tranexamic acid after peroral administration.", "content": "After a single oral dose of 25 mg/kg tranexamic acid in 37 patients with cataract as the sole eye disease, the concentration of the drug was measured in both the serum and the aqueous at various intervals following intake. The serum concentration was highest after three h (average 15.44 mg/l), but a trace of tranexamic acid (0.7 mg/l) could still be found after 19 h. In the aqueous, the concentration was likewise highest after three h (average 1.62 mg/l), but, following this, the fall in tranexamic acid concentration was very gradual and after 19 h was found to be 1.3 mg/l. In two patients who had received 25 mg/kg three times daily for three days, an aqueous concentration of 2.3 mg/l was found eight h after the final intake.", "contents": "Serum and aqueous humour concentration of tranexamic acid after peroral administration. After a single oral dose of 25 mg/kg tranexamic acid in 37 patients with cataract as the sole eye disease, the concentration of the drug was measured in both the serum and the aqueous at various intervals following intake. The serum concentration was highest after three h (average 15.44 mg/l), but a trace of tranexamic acid (0.7 mg/l) could still be found after 19 h. In the aqueous, the concentration was likewise highest after three h (average 1.62 mg/l), but, following this, the fall in tranexamic acid concentration was very gradual and after 19 h was found to be 1.3 mg/l. In two patients who had received 25 mg/kg three times daily for three days, an aqueous concentration of 2.3 mg/l was found eight h after the final intake."}
{"id": "PMID:474090", "title": "Age changes in the skeleton of the human lens.", "content": "The cytoskeleton of the human lens (newborn, 24 year-old and 80 year-old) was studied by morphological methods. Actin and intermediate (10 nm) filaments were identified in the epithelial cells of all the lenses. In the newborn lens intermediate filaments and chains of protein were found in cortical and nuclear fiber cells. Many intermediate filaments and protein chains in the elongated form were observed in the superficial cortical fiber cells of the 24 year-old lens. However, in the deep cortical cells the number of intermediate filaments decreased and the protein chains became more coiled and aggregated. In the nuclear region the protein chains were markedly aggregated and intermediate filaments were absent. The 80 year-old fiber cells contained only protein aggregates in the fiber cells. While the size of the chain backbone corresponds to actin, the conclusive demonstration that it is actin remains to be shown.", "contents": "Age changes in the skeleton of the human lens. The cytoskeleton of the human lens (newborn, 24 year-old and 80 year-old) was studied by morphological methods. Actin and intermediate (10 nm) filaments were identified in the epithelial cells of all the lenses. In the newborn lens intermediate filaments and chains of protein were found in cortical and nuclear fiber cells. Many intermediate filaments and protein chains in the elongated form were observed in the superficial cortical fiber cells of the 24 year-old lens. However, in the deep cortical cells the number of intermediate filaments decreased and the protein chains became more coiled and aggregated. In the nuclear region the protein chains were markedly aggregated and intermediate filaments were absent. The 80 year-old fiber cells contained only protein aggregates in the fiber cells. While the size of the chain backbone corresponds to actin, the conclusive demonstration that it is actin remains to be shown."}
{"id": "PMID:474091", "title": "Vitreous cutter in anterior segment surgery.", "content": "Experience has been gained in operating upon a variety of anterior segment disorders using a Grieshaber vitreous cutter. The results and complications are briefly discussed.", "contents": "Vitreous cutter in anterior segment surgery. Experience has been gained in operating upon a variety of anterior segment disorders using a Grieshaber vitreous cutter. The results and complications are briefly discussed."}
{"id": "PMID:474092", "title": "Flicker comparison of fundus photographs. A technical note.", "content": "A method for comparison of two fundus transparencies by means of alternating flickering light is described. Spurious differences between the pictures are reduced by making the exposures in a fixed position of the pulse cycle. Small haemorrhages are readily detected. Pulse variations of the vessels and changes in size of cup and disc can be measured.", "contents": "Flicker comparison of fundus photographs. A technical note. A method for comparison of two fundus transparencies by means of alternating flickering light is described. Spurious differences between the pictures are reduced by making the exposures in a fixed position of the pulse cycle. Small haemorrhages are readily detected. Pulse variations of the vessels and changes in size of cup and disc can be measured."}
{"id": "PMID:474093", "title": "Parathyroid hormone secretion after operative bone trauma.", "content": "Skeletal trauma in man (operations for scolioses and femoral shortening) results in a significant increase in parathyroid hormone secretion beginning the day after the operation and lasting for more than 1 week. A concurrent decrease in serum calcium, dependent on the postoperative lowering of serum albumin, was also observed.", "contents": "Parathyroid hormone secretion after operative bone trauma. Skeletal trauma in man (operations for scolioses and femoral shortening) results in a significant increase in parathyroid hormone secretion beginning the day after the operation and lasting for more than 1 week. A concurrent decrease in serum calcium, dependent on the postoperative lowering of serum albumin, was also observed."}
{"id": "PMID:474094", "title": "Effects of cloxacillin, doxycycline, fusidic acid and lincomycin on the mechanical properties of bone and skin in young rats.", "content": "The influence of cloxacillin, doxycycline, fuside acid and lincomycin on the mechanical properties of bone and skin in young rats was examined. The concentrations of the antibiotics in plasma corresponded to therapeutic levels in man. After 14 days of medication the weights of the rats receiving cloxacillin or doxycycline were significantly less when compared with the controls. The doxycycline, the fusidic acid and the lincomycin treated rats had reduced longitudinal growth of femur and reduced tensile strength of intact skin. No differences between any of the antibiotic groups and the control group were found in the tensile strength of incisional skin wounds or in the mechanical properties of the femur and tibia.", "contents": "Effects of cloxacillin, doxycycline, fusidic acid and lincomycin on the mechanical properties of bone and skin in young rats. The influence of cloxacillin, doxycycline, fuside acid and lincomycin on the mechanical properties of bone and skin in young rats was examined. The concentrations of the antibiotics in plasma corresponded to therapeutic levels in man. After 14 days of medication the weights of the rats receiving cloxacillin or doxycycline were significantly less when compared with the controls. The doxycycline, the fusidic acid and the lincomycin treated rats had reduced longitudinal growth of femur and reduced tensile strength of intact skin. No differences between any of the antibiotic groups and the control group were found in the tensile strength of incisional skin wounds or in the mechanical properties of the femur and tibia."}
{"id": "PMID:474095", "title": "A case of Albright's syndrome treated with calcitonin.", "content": "A 23-year-old woman with Albright's syndrome (polyostotic fibrous dysplasia of bone, precocious puberty and irregular cutaneous pigmentations) had sustained multiple fractures and was grossly disabled. Evaluation disclosed markedly raised serum alkaline phosphatases and a high urinary excretion of hydroxyproline, suggesting an accelerated bone turnover, while calcium metabolism was virtually undisturbed. During 12 months therapy with calcitonin, however, no apparent benefit was recorded and there was no evidence of any significant metabolic effects of the treatment. Initial discomfort with nausea and vomiting disappeared after dose reduction whereas diffuse bone and muscle pain, which gradually increased after a few months treatment, did not subside until after cessation of the therapy.", "contents": "A case of Albright's syndrome treated with calcitonin. A 23-year-old woman with Albright's syndrome (polyostotic fibrous dysplasia of bone, precocious puberty and irregular cutaneous pigmentations) had sustained multiple fractures and was grossly disabled. Evaluation disclosed markedly raised serum alkaline phosphatases and a high urinary excretion of hydroxyproline, suggesting an accelerated bone turnover, while calcium metabolism was virtually undisturbed. During 12 months therapy with calcitonin, however, no apparent benefit was recorded and there was no evidence of any significant metabolic effects of the treatment. Initial discomfort with nausea and vomiting disappeared after dose reduction whereas diffuse bone and muscle pain, which gradually increased after a few months treatment, did not subside until after cessation of the therapy."}
{"id": "PMID:474096", "title": "Normal grip strength.", "content": "The normal grip strength was determined with the Martin Vigorimeter in 450 men and women aged 21--65 years. The grip strength decreased steadily with increasing age. Men were stronger than women and in both sexes the dominant hand was the strongest. The ratio dominant/non-dominant hand varied only slightly with age and sex and it could thus be a useful parameter in evaluation of grip strength under pathological conditions.", "contents": "Normal grip strength. The normal grip strength was determined with the Martin Vigorimeter in 450 men and women aged 21--65 years. The grip strength decreased steadily with increasing age. Men were stronger than women and in both sexes the dominant hand was the strongest. The ratio dominant/non-dominant hand varied only slightly with age and sex and it could thus be a useful parameter in evaluation of grip strength under pathological conditions."}
{"id": "PMID:474097", "title": "Clavicular function.", "content": "This is a survey of the functional variation of the clavicle, in particular with regard to its adaptability, the bone being an essential link in the shoulder girdle. The presence of a clavicle, and its shape, size and position are discussed from the point of view of stability and mobility, and the role of the bone regarding circulation, pulmonary ventilation and muscular tension is pointed out. It is stated that the clavicle contributes significantly to the stability of the shoulder girdle, and that in man the presence of this bone represents an increase in the degree of freedom of the upper extremity mobility pattern, dependent on the transversal shape of the thoracic diameter. The precondition for the clavicle to function appropriately is that its curvature fits in with the ligamentous pull.", "contents": "Clavicular function. This is a survey of the functional variation of the clavicle, in particular with regard to its adaptability, the bone being an essential link in the shoulder girdle. The presence of a clavicle, and its shape, size and position are discussed from the point of view of stability and mobility, and the role of the bone regarding circulation, pulmonary ventilation and muscular tension is pointed out. It is stated that the clavicle contributes significantly to the stability of the shoulder girdle, and that in man the presence of this bone represents an increase in the degree of freedom of the upper extremity mobility pattern, dependent on the transversal shape of the thoracic diameter. The precondition for the clavicle to function appropriately is that its curvature fits in with the ligamentous pull."}
{"id": "PMID:474098", "title": "Spontaneous dislocation of the sterno-clavicular joint.", "content": "A description is given of 22 patients, with a spontaneous forward subluxation of the sternal end of the clavicle, seen over a 5-year period. The majority were middle-aged women presenting with a lump at the root of the neck, which was usually associated with little or no discomfort. There was no history of injury and no interference with shoulder function. The diagnosis was obvious on clinical examination and no treatment was required other than explanation, reassurance and simple analgesia. In no case was the correct diagnosis made by the referring physician and many of the patients were unduly concerned by the nature of the swelling. The sterno-clavicular joint is a synovial articulation and consequently it may be involved in rheumati arthropathy. A condition of non-infective subacute arthritis of the sterno-clavicular joint has been described and is similar to that reported here but in contrast to the previous report we believe that the changes which occur in the joint are secondary to its subluxation.", "contents": "Spontaneous dislocation of the sterno-clavicular joint. A description is given of 22 patients, with a spontaneous forward subluxation of the sternal end of the clavicle, seen over a 5-year period. The majority were middle-aged women presenting with a lump at the root of the neck, which was usually associated with little or no discomfort. There was no history of injury and no interference with shoulder function. The diagnosis was obvious on clinical examination and no treatment was required other than explanation, reassurance and simple analgesia. In no case was the correct diagnosis made by the referring physician and many of the patients were unduly concerned by the nature of the swelling. The sterno-clavicular joint is a synovial articulation and consequently it may be involved in rheumati arthropathy. A condition of non-infective subacute arthritis of the sterno-clavicular joint has been described and is similar to that reported here but in contrast to the previous report we believe that the changes which occur in the joint are secondary to its subluxation."}
{"id": "PMID:474099", "title": "Electroencephalographic investigation of patients with adolescent idiopathic scoliosis.", "content": "An EEG study was carried out on 57 children, aged 10--16 years, with idiopathic scoliosis. Treatment (brace or surgery) was required in 37 cases; 20 were being observed only. Controls were 10- to 16-year-old healthy children meeting well-defined criteria of normality. Thirty-four of the scoliotics fulfilled these criteria, thus providing a group matching the controls except for the presence of scoliosis. Thus, any differences occurring could, with reasonable probability, be referred to the disease in question. The comparison with healthy children showed a generally greater EEG pathology in the scoliotics. This was the case in all patients, even those meeting the same criteria of normality as the controls. A highly significant difference was noted in the occurrence of paroxysmal activity. Type and localization of abnormally (bilaterally synchronous discharges) indicated involvement of subcortical structures. Comparison within the scoliotic group showed that EEG abnormalities were not correlated with the magnitude of the disease except for the variable \"paroxysmal activity at rest\". Patients submitted to observation only presented paroxysmal activity significantly more often than patients requiring treatment. The localization of abnormality within the hemispheres did not agree or disagree systematically with the convexity of the curve. A possible relationship between the higher incidence of scoliosis in females and the higher sensitivity to afferent stimuli as found in the female EEG is also discussed.", "contents": "Electroencephalographic investigation of patients with adolescent idiopathic scoliosis. An EEG study was carried out on 57 children, aged 10--16 years, with idiopathic scoliosis. Treatment (brace or surgery) was required in 37 cases; 20 were being observed only. Controls were 10- to 16-year-old healthy children meeting well-defined criteria of normality. Thirty-four of the scoliotics fulfilled these criteria, thus providing a group matching the controls except for the presence of scoliosis. Thus, any differences occurring could, with reasonable probability, be referred to the disease in question. The comparison with healthy children showed a generally greater EEG pathology in the scoliotics. This was the case in all patients, even those meeting the same criteria of normality as the controls. A highly significant difference was noted in the occurrence of paroxysmal activity. Type and localization of abnormally (bilaterally synchronous discharges) indicated involvement of subcortical structures. Comparison within the scoliotic group showed that EEG abnormalities were not correlated with the magnitude of the disease except for the variable \"paroxysmal activity at rest\". Patients submitted to observation only presented paroxysmal activity significantly more often than patients requiring treatment. The localization of abnormality within the hemispheres did not agree or disagree systematically with the convexity of the curve. A possible relationship between the higher incidence of scoliosis in females and the higher sensitivity to afferent stimuli as found in the female EEG is also discussed."}
{"id": "PMID:474100", "title": "Moir\u00e9 topography for the diagnosis and documentation of scoliosis.", "content": "Moir\u00e9 topography is a biosteriometric method, which produces a three-dimensional image of the shape of the trunk. In structural scoliosis an asymmetry of the two halves of the back is seen. This method has been studied and the range of the asymmetry of the moire pattern has been compared with clinical observations and X-ray findings in 216 cases with structural scoliosis. A statistically significant correlation between these three ways of describing a structural scoliosis was found. Because of the sensitivity of the moire method an asymmetry of at least one fringe interval could be regarded as a positive result. All the observed asymmetries less than one fringe interval had a lateral deviation of the spine of less than 10 degrees according to Cobb when X-rayed examined. The moire method seems to be very suitable for the screening of structural scoliosis, owing to its ability to detect and document even small deformities by photography and the simplicity of the technique which can be carried out even by staff without medical training.", "contents": "Moir\u00e9 topography for the diagnosis and documentation of scoliosis. Moir\u00e9 topography is a biosteriometric method, which produces a three-dimensional image of the shape of the trunk. In structural scoliosis an asymmetry of the two halves of the back is seen. This method has been studied and the range of the asymmetry of the moire pattern has been compared with clinical observations and X-ray findings in 216 cases with structural scoliosis. A statistically significant correlation between these three ways of describing a structural scoliosis was found. Because of the sensitivity of the moire method an asymmetry of at least one fringe interval could be regarded as a positive result. All the observed asymmetries less than one fringe interval had a lateral deviation of the spine of less than 10 degrees according to Cobb when X-rayed examined. The moire method seems to be very suitable for the screening of structural scoliosis, owing to its ability to detect and document even small deformities by photography and the simplicity of the technique which can be carried out even by staff without medical training."}
{"id": "PMID:474101", "title": "The effect of physical training on exercise ability in adolescent idiopathic scoliosis.", "content": "Twenty subjects with adolescent idiopathic scoliosis, aged 11--17 years, were studied before spinal fusion. Half the patients underwent a programme of daily physical exercises for 6--10 weeks. Before and after this period all the patients carried out a range of lung function tests and a progressive exercise test. There were no significant differences in any of the results between the control group and the training group. The cardiac and respiratory complications of spinal fusion are therefore unlikely to be lessened by preoperative physical training, except in selected subjects.", "contents": "The effect of physical training on exercise ability in adolescent idiopathic scoliosis. Twenty subjects with adolescent idiopathic scoliosis, aged 11--17 years, were studied before spinal fusion. Half the patients underwent a programme of daily physical exercises for 6--10 weeks. Before and after this period all the patients carried out a range of lung function tests and a progressive exercise test. There were no significant differences in any of the results between the control group and the training group. The cardiac and respiratory complications of spinal fusion are therefore unlikely to be lessened by preoperative physical training, except in selected subjects."}
{"id": "PMID:474102", "title": "Neonatal hip instability. Reason for failure of early abduction treatment.", "content": "A series of nine children with hip joint instability in 17 hips, diagnosed neonatally, is presented. Seven had bilateral idiopathic instability and two instability secondary to arthrogryposis, one of them bilateral. After reduction seven of the children (14 hips) were treated with abduction devices, which in all cases did not lead to stability in one or both hips. In these cases arthrography revealed that closed reduction was impossible due to narrowing of the joint capsule (hour-glass shape) and the interposition of a capsular fold including the acetabular labrum. The same types of changes were seen within 1 or 2 months after birth in three hips which had had no abduction treatment before arthrography. At open reduction of 11 hips it was found that the narrowing of the capsule was caused by the tendons of the iliopsoas and rectus femoris muscles. Excision of the capsular fold (labrum) was not necessary. The femoral head was deformed and anteverted. Failure of conservative treatment can be due either to incomplete reduction or to inadequate immobilization. Our analysis has shown that the most probable reason is incomplete reduction due to interposition. An obstacle to reduction should be suspected if abduction is restricted at birth, if primary reduction is difficult and the position difficult to maintain or if instability persists after 8 weeks of treatment. On the basis of our material the incidence of such an impediment to reduction was 0.08 per thousand births in the region studied during a 5-year period.", "contents": "Neonatal hip instability. Reason for failure of early abduction treatment. A series of nine children with hip joint instability in 17 hips, diagnosed neonatally, is presented. Seven had bilateral idiopathic instability and two instability secondary to arthrogryposis, one of them bilateral. After reduction seven of the children (14 hips) were treated with abduction devices, which in all cases did not lead to stability in one or both hips. In these cases arthrography revealed that closed reduction was impossible due to narrowing of the joint capsule (hour-glass shape) and the interposition of a capsular fold including the acetabular labrum. The same types of changes were seen within 1 or 2 months after birth in three hips which had had no abduction treatment before arthrography. At open reduction of 11 hips it was found that the narrowing of the capsule was caused by the tendons of the iliopsoas and rectus femoris muscles. Excision of the capsular fold (labrum) was not necessary. The femoral head was deformed and anteverted. Failure of conservative treatment can be due either to incomplete reduction or to inadequate immobilization. Our analysis has shown that the most probable reason is incomplete reduction due to interposition. An obstacle to reduction should be suspected if abduction is restricted at birth, if primary reduction is difficult and the position difficult to maintain or if instability persists after 8 weeks of treatment. On the basis of our material the incidence of such an impediment to reduction was 0.08 per thousand births in the region studied during a 5-year period."}
{"id": "PMID:474103", "title": "Long term results of surgery for non-acute anteromedial rotatory instability of the knee.", "content": "Seventy-eight patients treated by extraarticular reconstruction including pes anserinus transfer for anteromedial rotatory instability of the knee were reinvestigated 16--47 months (mean 28) after operation. Function before and after operation was assessed by means of a knee rating score. Of these patients, 94 per cent showed a higher score after operation. Twenty per cent showed full recovery with no limitation of knee function whatsoever. The follow-up score was higher with preserved medial meniscus function than when this structure had been removed. When not initially torn, the medial meniscus tended to become involved with time. Mild laxity in extension, possibly indicative of a posterior cruciate injury previously underestimated or not observed, was found in 15 per cent of the patients. No correlation was found between late knee function and the interval between injury and operation or the interval between operation and follow-up examination. The long term results were good. Extraarticular reconstruction is thus indicated in cases of chronic rotatory instability of the anteromedial type. Routine meniscectomy is not recommended in these patients, however. Signs possibly indicating posterior cruciate ligament involvement should be carefully looked for, as even minor posterior cruciate injury, easily overlooked, may influence the late results.", "contents": "Long term results of surgery for non-acute anteromedial rotatory instability of the knee. Seventy-eight patients treated by extraarticular reconstruction including pes anserinus transfer for anteromedial rotatory instability of the knee were reinvestigated 16--47 months (mean 28) after operation. Function before and after operation was assessed by means of a knee rating score. Of these patients, 94 per cent showed a higher score after operation. Twenty per cent showed full recovery with no limitation of knee function whatsoever. The follow-up score was higher with preserved medial meniscus function than when this structure had been removed. When not initially torn, the medial meniscus tended to become involved with time. Mild laxity in extension, possibly indicative of a posterior cruciate injury previously underestimated or not observed, was found in 15 per cent of the patients. No correlation was found between late knee function and the interval between injury and operation or the interval between operation and follow-up examination. The long term results were good. Extraarticular reconstruction is thus indicated in cases of chronic rotatory instability of the anteromedial type. Routine meniscectomy is not recommended in these patients, however. Signs possibly indicating posterior cruciate ligament involvement should be carefully looked for, as even minor posterior cruciate injury, easily overlooked, may influence the late results."}
{"id": "PMID:474104", "title": "Recurrent instability of the ankle joint. Surgical repair by the Watson-Jones method.", "content": "Twenty-one ankle joints with recurrent lateral instability, treated with surgical repair by the Watson-Jones method, were included in a follow-up study 1--5 years after operation. Good results were achieved in 80 per cent. However, the results were not better than those after free dissection and direct suture of the ligamentous ruptures. It is therefore recommended that the more extensive Watson-Jones procedure be reserved for selected cases.", "contents": "Recurrent instability of the ankle joint. Surgical repair by the Watson-Jones method. Twenty-one ankle joints with recurrent lateral instability, treated with surgical repair by the Watson-Jones method, were included in a follow-up study 1--5 years after operation. Good results were achieved in 80 per cent. However, the results were not better than those after free dissection and direct suture of the ligamentous ruptures. It is therefore recommended that the more extensive Watson-Jones procedure be reserved for selected cases."}
{"id": "PMID:474107", "title": "Morphological alteration of the stria vascularis after administration of the diuretic bumetanide.", "content": "Chinchillas were given either a single injection of the diuretic bumetanide (18 mg/kg body weight) or saline-sodium hydroxide and sacrificed at 10 min, 1 hr and 24 hr after the injection. Slight stria edema was present at 10 min, marked edema at 1 hr and no edema 24 h after bumetanide. The edema began in the first cochlear turn at 10 min and spread to the second turn by 1 hr. Along with edema, marginal cell bulging, potential capillary constriction and the formation of marginal cell membranous structures occurred after bumetanide treatment.", "contents": "Morphological alteration of the stria vascularis after administration of the diuretic bumetanide. Chinchillas were given either a single injection of the diuretic bumetanide (18 mg/kg body weight) or saline-sodium hydroxide and sacrificed at 10 min, 1 hr and 24 hr after the injection. Slight stria edema was present at 10 min, marked edema at 1 hr and no edema 24 h after bumetanide. The edema began in the first cochlear turn at 10 min and spread to the second turn by 1 hr. Along with edema, marginal cell bulging, potential capillary constriction and the formation of marginal cell membranous structures occurred after bumetanide treatment."}
{"id": "PMID:474108", "title": "The influence of pneumatization of mastoid bone on caloric nystagmus response. A clinical study and a mathematical model.", "content": "Pursuing the problem whether and why the pneumatization of mastoid bone has any influence on caloric nystagmus, we examined 15 large or extensively (L.P.) and 15 poorly (P.P.) pneumatized subjects. Following water irrigation (44 degrees and 30 degrees C, 30 sec) we checked the parameters maximum SPV, max. frequency, latency, duration and time of max. response by ENG recordings. Both groups differed significantly for all parameters except duration (p less than 0.001). On comparing these findings with an earlier proposed model (Bock & Bromm, 1977) we could show a good correspondence for the P.P. group by doubling the parameter 'thermal diffusivity of mastoid bone'. An even better correspondence could be found by diminishing the parameter 'coefficient of temperature changes by perfusion'. The theoretical and practical implications and conclusions of our findings are discussed.", "contents": "The influence of pneumatization of mastoid bone on caloric nystagmus response. A clinical study and a mathematical model. Pursuing the problem whether and why the pneumatization of mastoid bone has any influence on caloric nystagmus, we examined 15 large or extensively (L.P.) and 15 poorly (P.P.) pneumatized subjects. Following water irrigation (44 degrees and 30 degrees C, 30 sec) we checked the parameters maximum SPV, max. frequency, latency, duration and time of max. response by ENG recordings. Both groups differed significantly for all parameters except duration (p less than 0.001). On comparing these findings with an earlier proposed model (Bock & Bromm, 1977) we could show a good correspondence for the P.P. group by doubling the parameter 'thermal diffusivity of mastoid bone'. An even better correspondence could be found by diminishing the parameter 'coefficient of temperature changes by perfusion'. The theoretical and practical implications and conclusions of our findings are discussed."}
{"id": "PMID:474109", "title": "The significance of the target frequency and the target speed in optokinetic nystagmus (OKN).", "content": "The significance of the frequency of the stimuli (target frequency) and the angular velocity of the stimuli (target speed) on horizontal optokinetic nystagmus (OKN) was investigated in 6 normal human subjects using target frequencies from 1/3 Hz to 24 Hz and target speeds from 5 degrees/sec to 120 degrees/sec. Regularity and reproductiveness of the OKN were obtained only in test conditions where each transit of targets was followed by an optokinetic response either as one beat or as a sequence of beats. This is called synchronous response and was found when the target frequency was below 3 Hz and the target speeds below 20--30 degrees/sec, depending on the actual frequency. At higher target frequencies and target speeds, the eyes were unable to take up every target, resulting in uneven responses (hyposynchronous response). Very low target frequencies and target speeds were likewise unsuitable due to drifting of the eyes from one target to another. A target frequency and a target speed close to the upper limit for synchronous response is advocated in clinical tests of OKN. 2 Hz and 20 degrees/sec is proposed as a suitable combination of frequency and target speed.", "contents": "The significance of the target frequency and the target speed in optokinetic nystagmus (OKN). The significance of the frequency of the stimuli (target frequency) and the angular velocity of the stimuli (target speed) on horizontal optokinetic nystagmus (OKN) was investigated in 6 normal human subjects using target frequencies from 1/3 Hz to 24 Hz and target speeds from 5 degrees/sec to 120 degrees/sec. Regularity and reproductiveness of the OKN were obtained only in test conditions where each transit of targets was followed by an optokinetic response either as one beat or as a sequence of beats. This is called synchronous response and was found when the target frequency was below 3 Hz and the target speeds below 20--30 degrees/sec, depending on the actual frequency. At higher target frequencies and target speeds, the eyes were unable to take up every target, resulting in uneven responses (hyposynchronous response). Very low target frequencies and target speeds were likewise unsuitable due to drifting of the eyes from one target to another. A target frequency and a target speed close to the upper limit for synchronous response is advocated in clinical tests of OKN. 2 Hz and 20 degrees/sec is proposed as a suitable combination of frequency and target speed."}
{"id": "PMID:474110", "title": "Myringotomy in the treatment of acute otitis media in children.", "content": "The treatment of acute otitis media was studied in 158 children. All children (mean age 4 years) received penicillin orally 80 000--100 000 IU per day for 10 days. Myringotomy was performed on 68 children at the time of the diagnosis. The other 90 children were treated with penicillin and ear drops. The bacteriological findings from the nasopharynx at the time of diagnosis were equivalent in both groups. After 2 weeks, 42% of the children without myringotomy and 71% of the children with myringotomy were cured. The children who were not cured were treated with amoxicillin for 10 days. Four weeks after diagnosis 71% and 90% of the children respectively were cured. The differences between the two groups are significant. The observations indicate that myringotomy clearly accelerates the recovery from acute otitis media.", "contents": "Myringotomy in the treatment of acute otitis media in children. The treatment of acute otitis media was studied in 158 children. All children (mean age 4 years) received penicillin orally 80 000--100 000 IU per day for 10 days. Myringotomy was performed on 68 children at the time of the diagnosis. The other 90 children were treated with penicillin and ear drops. The bacteriological findings from the nasopharynx at the time of diagnosis were equivalent in both groups. After 2 weeks, 42% of the children without myringotomy and 71% of the children with myringotomy were cured. The children who were not cured were treated with amoxicillin for 10 days. Four weeks after diagnosis 71% and 90% of the children respectively were cured. The differences between the two groups are significant. The observations indicate that myringotomy clearly accelerates the recovery from acute otitis media."}
{"id": "PMID:474111", "title": "Non-traumatic cerebrospinal rhinorrhea and chondrodystrophy.", "content": "Two chondrodystrophic males have developed a non-traumatic cerebrospinal rhinorrhea within their second decade. Hydrocephalus was the cause of cerebrospinal fluid leakage in both cases. To lower the intracranial pressure the neurosurgeon performed a ventriculocardiostomy. The fistula was closed by the rhinosurgical extradural route. With respect to the atrophic brain in cases of hydrocephalus, the fronto-nasal approach appeared to be the best and less traumatic way of exposing the frontal base of the skull.", "contents": "Non-traumatic cerebrospinal rhinorrhea and chondrodystrophy. Two chondrodystrophic males have developed a non-traumatic cerebrospinal rhinorrhea within their second decade. Hydrocephalus was the cause of cerebrospinal fluid leakage in both cases. To lower the intracranial pressure the neurosurgeon performed a ventriculocardiostomy. The fistula was closed by the rhinosurgical extradural route. With respect to the atrophic brain in cases of hydrocephalus, the fronto-nasal approach appeared to be the best and less traumatic way of exposing the frontal base of the skull."}
{"id": "PMID:474112", "title": "Physiological activation of the stapedius muscle in Gallus gallus.", "content": "The function of the avian middle ear muscle was investigated in the chicken, Gallus gallus (domesticus). The avian species offers excellent conditions for study of middle ear muscle function since it possesses a single middle ear muscle, the stapedius, which is located extracranially. Electromyograms (EMG), measurements of impedance change, and volume change in the middle ear cavity were used to assess the muscle's activity. The results showed that the middle ear muscle of Gallus does not exhibit an acoustic reflex. However, the stapedius is regularly activated during the animal's own vocalization. Measurements of the EMG and volume change showed the stapedial activity to increase systematically with increases in the vocal sound level. The use of volume change as a measure of stapedius function was found to be highly suitable in the present experiments in that it allows for measurements of the magnitude of the stapedius contraction without altering the intact physiological state of the middle ear, and is insensitive to the ambient noise and vocal sounds, that hamper the impedance technique.", "contents": "Physiological activation of the stapedius muscle in Gallus gallus. The function of the avian middle ear muscle was investigated in the chicken, Gallus gallus (domesticus). The avian species offers excellent conditions for study of middle ear muscle function since it possesses a single middle ear muscle, the stapedius, which is located extracranially. Electromyograms (EMG), measurements of impedance change, and volume change in the middle ear cavity were used to assess the muscle's activity. The results showed that the middle ear muscle of Gallus does not exhibit an acoustic reflex. However, the stapedius is regularly activated during the animal's own vocalization. Measurements of the EMG and volume change showed the stapedial activity to increase systematically with increases in the vocal sound level. The use of volume change as a measure of stapedius function was found to be highly suitable in the present experiments in that it allows for measurements of the magnitude of the stapedius contraction without altering the intact physiological state of the middle ear, and is insensitive to the ambient noise and vocal sounds, that hamper the impedance technique."}
{"id": "PMID:474113", "title": "Embryonal rhabdomyosarcoma of the middle ear.", "content": "An embryonic sarcoma of the middle ear occurring in a child has been described. The mainstay of treatment has been triple therapy. The child is well 4 years and 3 months after diagnosis.", "contents": "Embryonal rhabdomyosarcoma of the middle ear. An embryonic sarcoma of the middle ear occurring in a child has been described. The mainstay of treatment has been triple therapy. The child is well 4 years and 3 months after diagnosis."}
{"id": "PMID:474114", "title": "Role of the tensor veli palatini muscle in movement of the soft palate.", "content": "To examine the role of the tensor veli palatini muscle in palatal movement, we conducted 1) quantitative measurement of palatal movement by selective stimulation of the tensor and levator muscles, and 2) EMG recording of the two muscles during phonation. The results were: 1) negligible palatal movement upon tensor stimulation, despite a marked velar elevation by levator stimulation; and 2) little EMG activity of the tensor and marked EMG activity of the levator during phonation. It was concluded that the tensor plays no role in the palatal function.", "contents": "Role of the tensor veli palatini muscle in movement of the soft palate. To examine the role of the tensor veli palatini muscle in palatal movement, we conducted 1) quantitative measurement of palatal movement by selective stimulation of the tensor and levator muscles, and 2) EMG recording of the two muscles during phonation. The results were: 1) negligible palatal movement upon tensor stimulation, despite a marked velar elevation by levator stimulation; and 2) little EMG activity of the tensor and marked EMG activity of the levator during phonation. It was concluded that the tensor plays no role in the palatal function."}
{"id": "PMID:474115", "title": "Laryngeal chondrosarcoma in Sweden.", "content": "Between 1958 and 1972, 5 cases of posterior cricoid chondrosarcoma were reported to the Swedish Cancer Registry. These cases, and one recently observed case of laryngeal chondrosarcoma, are presented in this study of one female and five male patients. The tumours grow slowly and recur locally, but have not metastasized, and none of the patients has died from tumour disease. The clinical and morphological findings indicate that these tumours represent secondary chondrosarcomas developing from pre-existing benign ecchondromas. The reason for the uniformity as to localization is not known. The microscopic appearance varied in different parts of the same tumour. The presently obtained data and review of the literature indicate that laryngeal chondrosarcomas are extremely rare, locally invasive, but usually not metastasizing tumours. Conservative laryngeal operation is suggested as primary treatment. Total laryngectomy is necessary only after local recurrences involving a major portion of the cricoid cartilage.", "contents": "Laryngeal chondrosarcoma in Sweden. Between 1958 and 1972, 5 cases of posterior cricoid chondrosarcoma were reported to the Swedish Cancer Registry. These cases, and one recently observed case of laryngeal chondrosarcoma, are presented in this study of one female and five male patients. The tumours grow slowly and recur locally, but have not metastasized, and none of the patients has died from tumour disease. The clinical and morphological findings indicate that these tumours represent secondary chondrosarcomas developing from pre-existing benign ecchondromas. The reason for the uniformity as to localization is not known. The microscopic appearance varied in different parts of the same tumour. The presently obtained data and review of the literature indicate that laryngeal chondrosarcomas are extremely rare, locally invasive, but usually not metastasizing tumours. Conservative laryngeal operation is suggested as primary treatment. Total laryngectomy is necessary only after local recurrences involving a major portion of the cricoid cartilage."}
{"id": "PMID:474116", "title": "Work of nasal breathing: measurement of each nostril independently using a split mask.", "content": "The work of nasal breathing was determined in human subjects as a measure of impedance to respiratory airflow. The nasal cavities were examined separately and simultaneously with a split mask; flow and pressure signals were fed to a microprocessor for on-line computation and printout of respired volumes and work of nasal breathing. An alternating resistive nasal cycle of 3--4 hours' duration was demonstrated in the majority of normal, resting subjects. Reciprocity of the resistive changes in each nasal cavity maintained a constant total nasal respiratory work load of about 0.2 Joules/litre. Moderate changes in breathing rate and tidal volume had little influence on work. Inspiratory work was 1.6 times that of expiration. Increases in resistance of the dependent nostril were seen when the lateral decubitus position was adopted. Increase in cephalic venous pressure and pathological nasal obstruction increased the work of nasal breathing.", "contents": "Work of nasal breathing: measurement of each nostril independently using a split mask. The work of nasal breathing was determined in human subjects as a measure of impedance to respiratory airflow. The nasal cavities were examined separately and simultaneously with a split mask; flow and pressure signals were fed to a microprocessor for on-line computation and printout of respired volumes and work of nasal breathing. An alternating resistive nasal cycle of 3--4 hours' duration was demonstrated in the majority of normal, resting subjects. Reciprocity of the resistive changes in each nasal cavity maintained a constant total nasal respiratory work load of about 0.2 Joules/litre. Moderate changes in breathing rate and tidal volume had little influence on work. Inspiratory work was 1.6 times that of expiration. Increases in resistance of the dependent nostril were seen when the lateral decubitus position was adopted. Increase in cephalic venous pressure and pathological nasal obstruction increased the work of nasal breathing."}
{"id": "PMID:474117", "title": "Ultrastructural changes of the nerve elements following disruption of the organ of Corti. II. Nerve elements outside the organ of Corti.", "content": "Various stages of changes in the nerve fibers, spiral ganglion cells, and satellite cells from the guinea pig cochlea 3 to 137 days after perilymphatic perfusion with streptomycin solution (2 and 20%) were observed electron microscopically. Initially, the axoplasms of the cochlear nerve fibers became swollen or pyknotic. Then, the axons disappeared and myelin lamellae disrupted. The Schwann cells shrank and degenerated, though their basement membranes survived for a time. Regeneration of the cochlear nerve fibers began with extension of axonal sprouts into the tube of the basement membrane and surviving Schwann cells, which still contained myelin debris. Only one of the axonal sprouts matured for myelination. These regenerating cochlear nerve fibers were found in the osseous spiral lamina, modiolus and internal auditory meatus, but these fibers atrophied and disappeared afterward. Retrograde degeneration occurred in the olivo-cochlear bundle. Some of the efferent myelinated fibers also showed temporary regeneration.", "contents": "Ultrastructural changes of the nerve elements following disruption of the organ of Corti. II. Nerve elements outside the organ of Corti. Various stages of changes in the nerve fibers, spiral ganglion cells, and satellite cells from the guinea pig cochlea 3 to 137 days after perilymphatic perfusion with streptomycin solution (2 and 20%) were observed electron microscopically. Initially, the axoplasms of the cochlear nerve fibers became swollen or pyknotic. Then, the axons disappeared and myelin lamellae disrupted. The Schwann cells shrank and degenerated, though their basement membranes survived for a time. Regeneration of the cochlear nerve fibers began with extension of axonal sprouts into the tube of the basement membrane and surviving Schwann cells, which still contained myelin debris. Only one of the axonal sprouts matured for myelination. These regenerating cochlear nerve fibers were found in the osseous spiral lamina, modiolus and internal auditory meatus, but these fibers atrophied and disappeared afterward. Retrograde degeneration occurred in the olivo-cochlear bundle. Some of the efferent myelinated fibers also showed temporary regeneration."}
{"id": "PMID:474118", "title": "Long-term electrode implantation for recording cochlear electrical activity in guinea pigs.", "content": "Comparative measurements of the cochlear electrical activity over prolonged periods require long-term implantation of electrodes. During this time, changes in the electrode recording ability and/or impairment of the sound-conducting system, could erroneously be attributed to the experiment. To avoid this risk, an easy and reliable technique is described. The procedure uses the Fallopian canal as a route by which to approach the cochlear potentials' generators, while leaving the middle ear untouched.", "contents": "Long-term electrode implantation for recording cochlear electrical activity in guinea pigs. Comparative measurements of the cochlear electrical activity over prolonged periods require long-term implantation of electrodes. During this time, changes in the electrode recording ability and/or impairment of the sound-conducting system, could erroneously be attributed to the experiment. To avoid this risk, an easy and reliable technique is described. The procedure uses the Fallopian canal as a route by which to approach the cochlear potentials' generators, while leaving the middle ear untouched."}
{"id": "PMID:474119", "title": "Effects of local application of ototoxic antibiotics on cochlear potentials in guinea pigs.", "content": "The effects of neomycin, kanamycin and dihydrostreptomycin on the cochlear microphonic, the action potential of the auditory nerve and the endocochlear potential were studied in guinea pigs in which these drugs were locally administered by perfusion. These drugs suppressed the cochlear responses markedly when applied to the endolymph but were less effective when applied to the perilymph. The mechanisms of action of antibiotics on the hair cells of the organ of Corti are discussed.", "contents": "Effects of local application of ototoxic antibiotics on cochlear potentials in guinea pigs. The effects of neomycin, kanamycin and dihydrostreptomycin on the cochlear microphonic, the action potential of the auditory nerve and the endocochlear potential were studied in guinea pigs in which these drugs were locally administered by perfusion. These drugs suppressed the cochlear responses markedly when applied to the endolymph but were less effective when applied to the perilymph. The mechanisms of action of antibiotics on the hair cells of the organ of Corti are discussed."}
{"id": "PMID:474121", "title": "Incorporation of radioactive calcium into otolithic membranes of the guinea pig after aminoglycoside treatment.", "content": "The influence of neomycin and streptomycin on the calcium metabolism of the otolithic membranes was investigated in the guinea pig. After chronic treatment with either drug, animals were injected intraperitoneally with radioactive calcium. Retention of calcium in the serum was unaffected by drug treatment, as was the incorporation of radioactivity into bone (femur and otic capsule). Both drugs inhibited the calcium uptake into saccular and utricular otolithic membranes by 30 to 40%.", "contents": "Incorporation of radioactive calcium into otolithic membranes of the guinea pig after aminoglycoside treatment. The influence of neomycin and streptomycin on the calcium metabolism of the otolithic membranes was investigated in the guinea pig. After chronic treatment with either drug, animals were injected intraperitoneally with radioactive calcium. Retention of calcium in the serum was unaffected by drug treatment, as was the incorporation of radioactivity into bone (femur and otic capsule). Both drugs inhibited the calcium uptake into saccular and utricular otolithic membranes by 30 to 40%."}
{"id": "PMID:474122", "title": "Melanin capacity to accumulate drugs in the internal ear. A study on lidocaine, bupivacaine and chlorpromazine.", "content": "The distribution and retention of labelled lidocaine, bupivacaine, and chlorpromazine to melanin in the internal ear after intravenous and intraperitoneal injection were examined by whole-body autoradiography. Both young pigmented hooded rats and albino rats were studied. In the pigmented rats chlorpromazine showed the greatest accumulation, which was more pronounced in the cochlea than in the vestibular portion. The other two substances were evenly distributed in the internal ear. After a single injection of chlorpromazine and of bupivacaine these substances were still bound to the melanin of the internal ear after 14 days, which was the longest survival time. Lidocaine, on the other hand, had disappeared after only 4 days. Strong uptake and retention of the three substances were observed in the eyes of pigmented animals. In albino animals there was very weak, transient uptake in the internal ear of chlorpromazine and bupivacaine, but not of lidocaine. In studies in vitro on isolated bovine eye melanin there was considerably greater adsorption of chlorpromazine than of lidocaine and bupivacaine. An uptake was noted in the human eye in experiments in vitro. Clinical tests revealed no acute or late damage to hearing or sight after large doses of lidocaine. The participation of melanin in different basal labyrinthine functions such as the energy transfer mechanism and the sound protective mechanism is discussed in the light of the results obtained. Further, the theory is put forward that the melanin affinity of certain substances can be of both therapeutic and ototoxic importance.", "contents": "Melanin capacity to accumulate drugs in the internal ear. A study on lidocaine, bupivacaine and chlorpromazine. The distribution and retention of labelled lidocaine, bupivacaine, and chlorpromazine to melanin in the internal ear after intravenous and intraperitoneal injection were examined by whole-body autoradiography. Both young pigmented hooded rats and albino rats were studied. In the pigmented rats chlorpromazine showed the greatest accumulation, which was more pronounced in the cochlea than in the vestibular portion. The other two substances were evenly distributed in the internal ear. After a single injection of chlorpromazine and of bupivacaine these substances were still bound to the melanin of the internal ear after 14 days, which was the longest survival time. Lidocaine, on the other hand, had disappeared after only 4 days. Strong uptake and retention of the three substances were observed in the eyes of pigmented animals. In albino animals there was very weak, transient uptake in the internal ear of chlorpromazine and bupivacaine, but not of lidocaine. In studies in vitro on isolated bovine eye melanin there was considerably greater adsorption of chlorpromazine than of lidocaine and bupivacaine. An uptake was noted in the human eye in experiments in vitro. Clinical tests revealed no acute or late damage to hearing or sight after large doses of lidocaine. The participation of melanin in different basal labyrinthine functions such as the energy transfer mechanism and the sound protective mechanism is discussed in the light of the results obtained. Further, the theory is put forward that the melanin affinity of certain substances can be of both therapeutic and ototoxic importance."}
{"id": "PMID:474123", "title": "Vestibular neurectomy and saccus decompression surgery in Meniere's disease.", "content": "Results of vestibular neurectomy, total eighth nerve section, and saccus decompression in 42 patients with Meniere's disease are reported. Vestibular nerve section was found in isolated cases to be a very effective method of abolishing the symptom of vertigo. Hearing is not affected but may be lost owing to opening of the vertical canal or disruption of blood supply. Saccus surgery might be the surgical treatment of choice in early cases with good hearing but in patients with fixed non-fluctuating hearing loss, rehabilitation can be effected only by vestibular neurectomy. In bilateral cases either sac surgery or the use of vestibulotoxic drugs is advised.", "contents": "Vestibular neurectomy and saccus decompression surgery in Meniere's disease. Results of vestibular neurectomy, total eighth nerve section, and saccus decompression in 42 patients with Meniere's disease are reported. Vestibular nerve section was found in isolated cases to be a very effective method of abolishing the symptom of vertigo. Hearing is not affected but may be lost owing to opening of the vertical canal or disruption of blood supply. Saccus surgery might be the surgical treatment of choice in early cases with good hearing but in patients with fixed non-fluctuating hearing loss, rehabilitation can be effected only by vestibular neurectomy. In bilateral cases either sac surgery or the use of vestibulotoxic drugs is advised."}
{"id": "PMID:474124", "title": "Cervical and vestibular afferent control of oculomotor response in man.", "content": "Oculomotor response in the absence of vision has been compared in a group of 12 normal humans in two experimental conditions testing (a) the vestibulocular reflex by whole-body oscillation on a turntable, and (b) the cervico-ocular reflex by oscillation of the body with the head held stationary. The stimulus was a sinusoidal oscillation (peak angular velocity +/- 50 degrees/sec) at frequencies between 0.2 and 1.3 Hz. The slow-phase eye movements of the vestibulo-ocular response were compensatory for head movement and showed a mean gain of 0.54--0.90, increasing with frequency. The cervicoocular response was found to be very variable. The slow-phase eye movements were of low velocity (mean gain 0.05) and did not generally compensate for body movement. During neck torsion, some subjects exhibited large overall eye deviations composed of both slow and fast phase eye movements.", "contents": "Cervical and vestibular afferent control of oculomotor response in man. Oculomotor response in the absence of vision has been compared in a group of 12 normal humans in two experimental conditions testing (a) the vestibulocular reflex by whole-body oscillation on a turntable, and (b) the cervico-ocular reflex by oscillation of the body with the head held stationary. The stimulus was a sinusoidal oscillation (peak angular velocity +/- 50 degrees/sec) at frequencies between 0.2 and 1.3 Hz. The slow-phase eye movements of the vestibulo-ocular response were compensatory for head movement and showed a mean gain of 0.54--0.90, increasing with frequency. The cervicoocular response was found to be very variable. The slow-phase eye movements were of low velocity (mean gain 0.05) and did not generally compensate for body movement. During neck torsion, some subjects exhibited large overall eye deviations composed of both slow and fast phase eye movements."}
{"id": "PMID:474125", "title": "Caloric pattern test with special reference to failure of fixation-suppression.", "content": "Fixation-suppression of caloric nystagmus was studied in 1230 consecutive cases. An analysis of caloric nystagmus with failure of fixation-suppression (FFS) made it possible to classify patterns of FFS into three types: Type I: FFS was observed bilaterally in caloric nystagmus; Type II: FFS was recognized unilaterally on either side; Type III: FFS was seen in either direction of nystagmus. On the basis of these three types, the underlying neuroanatomical substrate responsible for FFS is discussed.", "contents": "Caloric pattern test with special reference to failure of fixation-suppression. Fixation-suppression of caloric nystagmus was studied in 1230 consecutive cases. An analysis of caloric nystagmus with failure of fixation-suppression (FFS) made it possible to classify patterns of FFS into three types: Type I: FFS was observed bilaterally in caloric nystagmus; Type II: FFS was recognized unilaterally on either side; Type III: FFS was seen in either direction of nystagmus. On the basis of these three types, the underlying neuroanatomical substrate responsible for FFS is discussed."}
{"id": "PMID:474127", "title": "A retrospective histological study of 669 cases of primary cutaneous malignant melanoma in clinical stage I. The consequences of a reclassification of the original group of lentigo maligna melanomas.", "content": "A selected series of primary malignant melanoma of the skin, clinical stage I, was originally classified according to Clark's system. The consistency of this classification was tested by two Brisbane pathologists who indicated that we had misinterpreted some cases of superficial spreading malignant melanoma as lentigo maligna melanoma. We have therefore reclassified the original group of 86 lentigo maligna melanomas. This resulted in a total series of 37 (5.5%) lentigo maligna melanomas, 301 (45%) superficial spreading malignant melanomas, 194 (29%) nodular malignant melanomas (unchanged) and 137 (20.5%) unclassifiable malignant melanomas. The diagnosis of lentigo maligna melanoma was not made unless the epidermis was atrophic and dermal solar elastosis was present. The new group of lentigo maligna melanomas is dominated by cases on the head among patients over 50 years of age (especially women). This is in better agreement with other studies than our previous findings. The relationship with tumour cell type, pigmentation, mitotic count, atypia, transsectional profile, level of invasion, ulceration, vascular invasion, lymphocyte infiltration and prognosis shown by the new groups of lentigo maligna melanoma and superficial spreading malignant melanoma indicates that the cases by which the diagnosis has been changed are relatively benign. Our previous conclusions are still valid. The lentigo maligna melanoma is still the most benign type and nodular malignant melanoma still the most malignant type of melanoma. The superficial spreading malignant melanoma still represents an intermediate tumour type, although it has deviated in the benign direction.", "contents": "A retrospective histological study of 669 cases of primary cutaneous malignant melanoma in clinical stage I. The consequences of a reclassification of the original group of lentigo maligna melanomas. A selected series of primary malignant melanoma of the skin, clinical stage I, was originally classified according to Clark's system. The consistency of this classification was tested by two Brisbane pathologists who indicated that we had misinterpreted some cases of superficial spreading malignant melanoma as lentigo maligna melanoma. We have therefore reclassified the original group of 86 lentigo maligna melanomas. This resulted in a total series of 37 (5.5%) lentigo maligna melanomas, 301 (45%) superficial spreading malignant melanomas, 194 (29%) nodular malignant melanomas (unchanged) and 137 (20.5%) unclassifiable malignant melanomas. The diagnosis of lentigo maligna melanoma was not made unless the epidermis was atrophic and dermal solar elastosis was present. The new group of lentigo maligna melanomas is dominated by cases on the head among patients over 50 years of age (especially women). This is in better agreement with other studies than our previous findings. The relationship with tumour cell type, pigmentation, mitotic count, atypia, transsectional profile, level of invasion, ulceration, vascular invasion, lymphocyte infiltration and prognosis shown by the new groups of lentigo maligna melanoma and superficial spreading malignant melanoma indicates that the cases by which the diagnosis has been changed are relatively benign. Our previous conclusions are still valid. The lentigo maligna melanoma is still the most benign type and nodular malignant melanoma still the most malignant type of melanoma. The superficial spreading malignant melanoma still represents an intermediate tumour type, although it has deviated in the benign direction."}
{"id": "PMID:474126", "title": "Ochratoxin A-induced mycotoxic porcine nephropathy: alterations in enzyme activity in tubular cells.", "content": "Mycotoxic porcine nephropathy was induced by p.o. administration of crystalline ochratoxin A for periods of 5 days, 3 months and 2 years. Enzyme activities of the renal tissue were studied histochemically. These were NADH-tetrazolium reductase, NADPH-tetrazolium reductase, lactate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, glucose-6-phosphate dehydrogenase, alpha-glycerophosphate dehydrogenase, unspecific acid phosphatase and unspecific alkaline phosphatase. The activity of NADH-tetrazolium reductase and succinate dehydrogenase was reduced in the proximal tubule of all nephrons after 5 days ochratoxin A exposure and remained reduced after 3 months and 2 years exposure. The effect of ochratoxin A on these enzymes would appear to cause the impairment of proximal tubular function and the morphological changes observed in the proximal tubule in ochratoxin A-induced mycotoxic porcine nephropathy. The localization of alterations in enzyme activity corresponds to the localization of ochratoxin A previously demonstrated in the kidney. The activities of NADPH-tetrazolium reductase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and unspecific alkaline phosphatase were reduced focally corresponding to the areas with focal tubular atrophy and the degree of reduction was roughly parallel to the degree of atrophy.", "contents": "Ochratoxin A-induced mycotoxic porcine nephropathy: alterations in enzyme activity in tubular cells. Mycotoxic porcine nephropathy was induced by p.o. administration of crystalline ochratoxin A for periods of 5 days, 3 months and 2 years. Enzyme activities of the renal tissue were studied histochemically. These were NADH-tetrazolium reductase, NADPH-tetrazolium reductase, lactate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, glucose-6-phosphate dehydrogenase, alpha-glycerophosphate dehydrogenase, unspecific acid phosphatase and unspecific alkaline phosphatase. The activity of NADH-tetrazolium reductase and succinate dehydrogenase was reduced in the proximal tubule of all nephrons after 5 days ochratoxin A exposure and remained reduced after 3 months and 2 years exposure. The effect of ochratoxin A on these enzymes would appear to cause the impairment of proximal tubular function and the morphological changes observed in the proximal tubule in ochratoxin A-induced mycotoxic porcine nephropathy. The localization of alterations in enzyme activity corresponds to the localization of ochratoxin A previously demonstrated in the kidney. The activities of NADPH-tetrazolium reductase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and unspecific alkaline phosphatase were reduced focally corresponding to the areas with focal tubular atrophy and the degree of reduction was roughly parallel to the degree of atrophy."}
{"id": "PMID:474128", "title": "Teratogenicity of intrauterine copper wire in mice. A histopathological study.", "content": "Immediately after the implantation of the fertilized ovum, on the 6th day of pregnancy a copperwire was placed in the right hand uterine section on pregnant C3H mice. The left section was similarly surgically traumatized in order to serve as a control. The mice were killed with chloroform on the 19th day of pregnancy and by means of a special histological screening technique the fetuses were examined with special reference to growth reduction, intrauterine fetal death and microscopic malformations. Differences in intrauterine fetal death were shown not to exist, nor was there any significant growth retardation in the copperwire containing uterine section. Certain microscopic defects were found with slightly increased incidence in the copper containing section, but the differences were not statistically significant.", "contents": "Teratogenicity of intrauterine copper wire in mice. A histopathological study. Immediately after the implantation of the fertilized ovum, on the 6th day of pregnancy a copperwire was placed in the right hand uterine section on pregnant C3H mice. The left section was similarly surgically traumatized in order to serve as a control. The mice were killed with chloroform on the 19th day of pregnancy and by means of a special histological screening technique the fetuses were examined with special reference to growth reduction, intrauterine fetal death and microscopic malformations. Differences in intrauterine fetal death were shown not to exist, nor was there any significant growth retardation in the copperwire containing uterine section. Certain microscopic defects were found with slightly increased incidence in the copper containing section, but the differences were not statistically significant."}
{"id": "PMID:474129", "title": "Repair in arterial tissue. Endothelial regrowth, subendothelial tissue changes and permeability in the healing rabbit thoracic aorta.", "content": "The role of the endothelium and subendothelial connective tissue in the permeability of a healing intima was studied by vital staining with Evans blue and transmission electron microscopy after severe mechanical lesion of the rabbit aorta. Reendothelialization, decreasing permeability, and organization of neointimal connective tissue were concomitant events in the healing processes. The determinant factor in decreasing permeability was reendothelialization with the formation of endothelial flaps and junctions. Changes in the subendothelial connective tissue seemed also to be factors that influenced the permeability.", "contents": "Repair in arterial tissue. Endothelial regrowth, subendothelial tissue changes and permeability in the healing rabbit thoracic aorta. The role of the endothelium and subendothelial connective tissue in the permeability of a healing intima was studied by vital staining with Evans blue and transmission electron microscopy after severe mechanical lesion of the rabbit aorta. Reendothelialization, decreasing permeability, and organization of neointimal connective tissue were concomitant events in the healing processes. The determinant factor in decreasing permeability was reendothelialization with the formation of endothelial flaps and junctions. Changes in the subendothelial connective tissue seemed also to be factors that influenced the permeability."}
{"id": "PMID:474130", "title": "Repair in arterial tissue. 2. Connective tissue changes following an embolectomy catheter lesion. The importance of the endothelial cells to repair and regeneration.", "content": "The neointimal hyperplasia following a severe mechanical lesion of the rabbit thoracic aorta was studied by vital staining with Evans blue and transmission electron microscopy. Neointimal tissue covered with endothelium contained organized laminated elastin-rich commective tissue. On the contrary neointimal connective tissue covered with pseudoendothelium was disorganized, with a tendency to fibrosis. Reendothelialization, re-establishment of intimal barrier function and formation of lamellated neointimal connective were parallel events. The importance of a intact subendothelial zone controlling healing processes is discussed. Interaction of endothelium and smooth muscle cells seems to be essential in the regulation of neointimal tissue formation and is probably implicated in a general vascular reactive pattern.", "contents": "Repair in arterial tissue. 2. Connective tissue changes following an embolectomy catheter lesion. The importance of the endothelial cells to repair and regeneration. The neointimal hyperplasia following a severe mechanical lesion of the rabbit thoracic aorta was studied by vital staining with Evans blue and transmission electron microscopy. Neointimal tissue covered with endothelium contained organized laminated elastin-rich commective tissue. On the contrary neointimal connective tissue covered with pseudoendothelium was disorganized, with a tendency to fibrosis. Reendothelialization, re-establishment of intimal barrier function and formation of lamellated neointimal connective were parallel events. The importance of a intact subendothelial zone controlling healing processes is discussed. Interaction of endothelium and smooth muscle cells seems to be essential in the regulation of neointimal tissue formation and is probably implicated in a general vascular reactive pattern."}
{"id": "PMID:474131", "title": "Seroepidemiology of H1N1 influenza: striking differences in the attack rate among young people.", "content": "The H1N1 influenza outbreak in winter 1977-78 covered the whole of Finland in a uniform fashion, whereas striking differences emerged between various population segments in the susceptible age group. The attack rate was 68 per cent among young servicemen who had been in the armed forces during the epidemic and 33 per cent among those who had entered the service after the epidemic, but only 6 per cent among pregnant women of the same age. Based on routine specimens sent to the laboratory for reasons unrelated to influenza, the attack rate was 4 per cent among children born in 1973-76 and increased by age up to 45 per cent among subjects born in 1957-62. The findings on pregnant women and small children suggest a novel mechanism of innate resistance operating specifically against H1N1 influenza.", "contents": "Seroepidemiology of H1N1 influenza: striking differences in the attack rate among young people. The H1N1 influenza outbreak in winter 1977-78 covered the whole of Finland in a uniform fashion, whereas striking differences emerged between various population segments in the susceptible age group. The attack rate was 68 per cent among young servicemen who had been in the armed forces during the epidemic and 33 per cent among those who had entered the service after the epidemic, but only 6 per cent among pregnant women of the same age. Based on routine specimens sent to the laboratory for reasons unrelated to influenza, the attack rate was 4 per cent among children born in 1973-76 and increased by age up to 45 per cent among subjects born in 1957-62. The findings on pregnant women and small children suggest a novel mechanism of innate resistance operating specifically against H1N1 influenza."}
{"id": "PMID:474132", "title": "Quantification of tetanus antitoxin in human sera. I. Counter-immunoelectrophoresis.", "content": "An extensive sero-epidemiological survey of immunity to tetanus is to be performed in Norway during 1978 and 1979 and thus a simple and reliable method for screening sera for content of tetanus antitoxin is needed. An improved counter-immunoelectrophoretic method for quantification of tetanus antitoxin is described. The toxin neutralization test in mice is considered to correlate well with protection in humans. Counter-immunoelectrophoresis has the advantage of using tetanus toxoid instead of toxin as well as being more rapid and less expensive. Specific antibodies of the IgG, IgM, and IgA classes could be read simultaneously for many sera. This may be of importance, since it is reported in the literature that only antibody of the IgG class is capable of neutralizing tetanus toxin in mice. Counter-immunoelectrophoresis is limited by being less sensitive than the toxin neutralization test in mice.", "contents": "Quantification of tetanus antitoxin in human sera. I. Counter-immunoelectrophoresis. An extensive sero-epidemiological survey of immunity to tetanus is to be performed in Norway during 1978 and 1979 and thus a simple and reliable method for screening sera for content of tetanus antitoxin is needed. An improved counter-immunoelectrophoretic method for quantification of tetanus antitoxin is described. The toxin neutralization test in mice is considered to correlate well with protection in humans. Counter-immunoelectrophoresis has the advantage of using tetanus toxoid instead of toxin as well as being more rapid and less expensive. Specific antibodies of the IgG, IgM, and IgA classes could be read simultaneously for many sera. This may be of importance, since it is reported in the literature that only antibody of the IgG class is capable of neutralizing tetanus toxin in mice. Counter-immunoelectrophoresis is limited by being less sensitive than the toxin neutralization test in mice."}
{"id": "PMID:474133", "title": "Quantification of tetanus antitoxin in human sera. II. Comparison of counter-immunoelectrophoresis and passive haemagglutination with toxin neutralization in mice.", "content": "We described recently an improved counter-immunoelectrophoretic method for quantification of tetanus antitoxin. The toxin neutralization test in mice is considered to correlate well with protection in humans. In the present study, the correlation coefficient between the two methods was 0.89. Sera containing more than 7.0 I.U./ml could be quantified directly by counter-immunoelectrophoresis, while sera containing less tetanus antitoxin had to be concentrated prior to quantification. The passive haemagglutination test was also compared with the toxin neutralization test in mice. The correlation coefficient between the two methods was 0.76.", "contents": "Quantification of tetanus antitoxin in human sera. II. Comparison of counter-immunoelectrophoresis and passive haemagglutination with toxin neutralization in mice. We described recently an improved counter-immunoelectrophoretic method for quantification of tetanus antitoxin. The toxin neutralization test in mice is considered to correlate well with protection in humans. In the present study, the correlation coefficient between the two methods was 0.89. Sera containing more than 7.0 I.U./ml could be quantified directly by counter-immunoelectrophoresis, while sera containing less tetanus antitoxin had to be concentrated prior to quantification. The passive haemagglutination test was also compared with the toxin neutralization test in mice. The correlation coefficient between the two methods was 0.76."}
{"id": "PMID:474141", "title": "The distribution of soluble copper- and zinc-binding proteins in goat liver.", "content": "The distribution of copper and zinc in the soluble proteins from goat liver after gel filtration were determined. Samples from normal slaughtered animals as well as from experimentally copper-loaded goats were tested. The copper- and zinc-binding proteins were mainly separated into 3 different fractions. The approximate molecular weights were greater than 65,000, 35,000 and 10,000. Varying amounts of zinc were eluted in a fourth fraction of molecular weight less than 2000. The results indicate that the distribution of copper and zinc among soluble metal-binding proteins are highly dependent on the total liver zinc concentration. In the majority of samples, with zinc levels within 20 to 45 microgram/g wet weight, the dominating copper- and zinc-binding protein fraction was the high-molecular weight fraction. This did not seem to be influenced by the total liver copper level. In the samples with high total zinc levels (greater than 60 microgram/g w.w.) there was a shift in protein distribution of both metals toward the low-molecular weight fractions.", "contents": "The distribution of soluble copper- and zinc-binding proteins in goat liver. The distribution of copper and zinc in the soluble proteins from goat liver after gel filtration were determined. Samples from normal slaughtered animals as well as from experimentally copper-loaded goats were tested. The copper- and zinc-binding proteins were mainly separated into 3 different fractions. The approximate molecular weights were greater than 65,000, 35,000 and 10,000. Varying amounts of zinc were eluted in a fourth fraction of molecular weight less than 2000. The results indicate that the distribution of copper and zinc among soluble metal-binding proteins are highly dependent on the total liver zinc concentration. In the majority of samples, with zinc levels within 20 to 45 microgram/g wet weight, the dominating copper- and zinc-binding protein fraction was the high-molecular weight fraction. This did not seem to be influenced by the total liver copper level. In the samples with high total zinc levels (greater than 60 microgram/g w.w.) there was a shift in protein distribution of both metals toward the low-molecular weight fractions."}
{"id": "PMID:474142", "title": "The loss of creatine phosphokinase (CK) from intramuscular injection sites in rabbits. A predictive tool for local toxicity.", "content": "The CK activity was measured in muscle tissue taken from the injected area (dorsal longissimus muscle) and the contralateral side of the injection site 72 hours after intramuscular injection into rabbits of 1 ml of different dilutions of propylene glycol or glycerol formal in distilled water or 0.9% saline. The total loss of CK activity from the injection site was calculated as the difference between the CK concentration in the normal muscle tissue and that of the injection site from the same animal. From the results the arbitrary amount of muscle tissue depleted of CK activity was further calculated and compared with the severity of the gross pathological findings. A large necrotic area at the injection site was present in all samples with more than 1 g of muscle tissue depleted of CK activity. Minor and probably acceptable pathological changes were found in samples with less than 1 g of muscle tissue depleted of CK activity. The local damaging effect of drug preparations for intramuscular use can thus be evaluated from the calculated amount of muscle tissue depleted of CK activity.", "contents": "The loss of creatine phosphokinase (CK) from intramuscular injection sites in rabbits. A predictive tool for local toxicity. The CK activity was measured in muscle tissue taken from the injected area (dorsal longissimus muscle) and the contralateral side of the injection site 72 hours after intramuscular injection into rabbits of 1 ml of different dilutions of propylene glycol or glycerol formal in distilled water or 0.9% saline. The total loss of CK activity from the injection site was calculated as the difference between the CK concentration in the normal muscle tissue and that of the injection site from the same animal. From the results the arbitrary amount of muscle tissue depleted of CK activity was further calculated and compared with the severity of the gross pathological findings. A large necrotic area at the injection site was present in all samples with more than 1 g of muscle tissue depleted of CK activity. Minor and probably acceptable pathological changes were found in samples with less than 1 g of muscle tissue depleted of CK activity. The local damaging effect of drug preparations for intramuscular use can thus be evaluated from the calculated amount of muscle tissue depleted of CK activity."}
{"id": "PMID:474143", "title": "Inhibition of 3H-dopamine accumulation in reserpinized and normal rat striatum.", "content": "The inhibitory potencies of 27 compounds on the accumulation of 3H-dopamine (DA) in synaptosome-rich striatal homogenates of normal and reserpinized rats were determined. It was found that some compounds, e.g. amphetamine derivatives, phenmetrazine, phenethylamine derivatives and tryptamine derivatives were considerably more potent in the reserpinized preparation than in the normal one. Other compounds, e.g. amfonelic acid, mazindol, EXP 561, benztropine, pipradrol, nomifensine, methylphenidate and cocaine had similar potencies in the two preparations. It is suggested that the compounds enhanced by reserpine are more potent as DA releasing agents than as inhibitors of the DA uptake, whereas the compounds in the other group are most potent as uptake inhibitors. Interestingly, these two groups completely agree with the two groups of central stimulatory agents, viz. the amphetamine-like and the methylphenidate-like drugs.", "contents": "Inhibition of 3H-dopamine accumulation in reserpinized and normal rat striatum. The inhibitory potencies of 27 compounds on the accumulation of 3H-dopamine (DA) in synaptosome-rich striatal homogenates of normal and reserpinized rats were determined. It was found that some compounds, e.g. amphetamine derivatives, phenmetrazine, phenethylamine derivatives and tryptamine derivatives were considerably more potent in the reserpinized preparation than in the normal one. Other compounds, e.g. amfonelic acid, mazindol, EXP 561, benztropine, pipradrol, nomifensine, methylphenidate and cocaine had similar potencies in the two preparations. It is suggested that the compounds enhanced by reserpine are more potent as DA releasing agents than as inhibitors of the DA uptake, whereas the compounds in the other group are most potent as uptake inhibitors. Interestingly, these two groups completely agree with the two groups of central stimulatory agents, viz. the amphetamine-like and the methylphenidate-like drugs."}
{"id": "PMID:474144", "title": "Fluoride and energy metabolism in LS cells.", "content": "Six mM fluoride had no effect on lactate production in LS cells incubated in Eagle's minimum essential medium. In Krebs-Ringer phosphate buffer with 10 mM glucose inhibition was found with 3mM fluoride. The fluoride effect was similar in aerobic and anaerobic conditions. Twelve mM fluoride had no inhibitory effect on ATP levels in LS cells.", "contents": "Fluoride and energy metabolism in LS cells. Six mM fluoride had no effect on lactate production in LS cells incubated in Eagle's minimum essential medium. In Krebs-Ringer phosphate buffer with 10 mM glucose inhibition was found with 3mM fluoride. The fluoride effect was similar in aerobic and anaerobic conditions. Twelve mM fluoride had no inhibitory effect on ATP levels in LS cells."}
{"id": "PMID:474145", "title": "Effect of sodium fluoride on protein and DNA synthesis, ornithine decarboxylase activity, and polyamine content in LS cells.", "content": "Sodium fluoride exhibited a dose dependent inhibitory effect on protein and DNA synthesis at concentrations from 1.3 mM in growing LS cells. The activity of ornithine decarboxylase (ODC) was slightly stimulated by 0.5 mM-NAF, but inhibited at 1.3 mM and above. The reduced enzyme activity seemed to be due to a reduced de novo formation of the enzyme caused by an inhibition of the protein synthesis. In spite of a reduction of ODC-activity, fluoride had no effect on the cellular polyamine content during the experimental period (10 hours).", "contents": "Effect of sodium fluoride on protein and DNA synthesis, ornithine decarboxylase activity, and polyamine content in LS cells. Sodium fluoride exhibited a dose dependent inhibitory effect on protein and DNA synthesis at concentrations from 1.3 mM in growing LS cells. The activity of ornithine decarboxylase (ODC) was slightly stimulated by 0.5 mM-NAF, but inhibited at 1.3 mM and above. The reduced enzyme activity seemed to be due to a reduced de novo formation of the enzyme caused by an inhibition of the protein synthesis. In spite of a reduction of ODC-activity, fluoride had no effect on the cellular polyamine content during the experimental period (10 hours)."}
{"id": "PMID:474146", "title": "The effect of fluoride on the cellular uptake and pool of amino acids.", "content": "Fluoride decreased the initial uptake of 2-aminoisobutyric acid (AIB) in LS cells only in high (supralethal) concentrations and after several hours of exposure. The steady state level of AIB seemed somewhat more sensitive as it was halved after 23 hours exposure to 6 mM-NaF. The cellular levels of some natural amino acids were also reduced by fluoride. However, cells resistant to fluoride showed equally lowered levels of amino acids, indicating that the observed effect of fluoride on cellular amino acid levels may be of little importance.", "contents": "The effect of fluoride on the cellular uptake and pool of amino acids. Fluoride decreased the initial uptake of 2-aminoisobutyric acid (AIB) in LS cells only in high (supralethal) concentrations and after several hours of exposure. The steady state level of AIB seemed somewhat more sensitive as it was halved after 23 hours exposure to 6 mM-NaF. The cellular levels of some natural amino acids were also reduced by fluoride. However, cells resistant to fluoride showed equally lowered levels of amino acids, indicating that the observed effect of fluoride on cellular amino acid levels may be of little importance."}
{"id": "PMID:474147", "title": "Effects of tetraethyl lead on the activities of drug metabolizing enzymes in different tissues of the rat.", "content": "The present study describes the effects of tetraethyl lead on various drug metabolizing enzymes in different tissues of the rat. Tetraethyl lead was administered intraperitoneally to rats (250 mumol/kg) on two consecutive days. The animals were killed on day 3. Tetraethyl lead-treatment decreased the concentration of hepatic cytochrome P-450 (to 45 per cent of the control), the hepatic activity of aryl hydrocarbon hydroxylase (to 41 per cent of the control) and ethoxycoumarin deethylase (to 45 per cent of the control). Epoxide hydratase activity was enhanced in the liver (1.3-fold), kidney (3.3-fold), and small intestinal mucosa (4.7-fold). The activity of glutathione S-transferase decreased in the liver (to 69 per cent of the control) but increased in the kidney (1.5-fold) and small intestinal mucosa (1.7-fold). The glucuronidation of o-aminophenol was enhanced (2.2-fold) in the kidney of tetraethyl lead treated rats. It is concluded that exposure to tetraethyl lead brings about widespread changes in the ability of mammals to detoxify foreign compounds.", "contents": "Effects of tetraethyl lead on the activities of drug metabolizing enzymes in different tissues of the rat. The present study describes the effects of tetraethyl lead on various drug metabolizing enzymes in different tissues of the rat. Tetraethyl lead was administered intraperitoneally to rats (250 mumol/kg) on two consecutive days. The animals were killed on day 3. Tetraethyl lead-treatment decreased the concentration of hepatic cytochrome P-450 (to 45 per cent of the control), the hepatic activity of aryl hydrocarbon hydroxylase (to 41 per cent of the control) and ethoxycoumarin deethylase (to 45 per cent of the control). Epoxide hydratase activity was enhanced in the liver (1.3-fold), kidney (3.3-fold), and small intestinal mucosa (4.7-fold). The activity of glutathione S-transferase decreased in the liver (to 69 per cent of the control) but increased in the kidney (1.5-fold) and small intestinal mucosa (1.7-fold). The glucuronidation of o-aminophenol was enhanced (2.2-fold) in the kidney of tetraethyl lead treated rats. It is concluded that exposure to tetraethyl lead brings about widespread changes in the ability of mammals to detoxify foreign compounds."}
{"id": "PMID:474148", "title": "Study of thioridazine cardiotoxic effects by means of His bundle activity recording.", "content": "The effect of thioridazine on sinusal automaticity, myocardial excitability and auriculoventricular conduction have been studied in dogs by measuring the spontaneous heart rate, the effective refractory period of the atrial contractile tissue, the time of auriculonodal and infrahisian conduction. Chloralose anaesthetized dogs were administered 10 mg/kg thioridazine hydrochloride via intravenous route. While sinusal automaticity was not altered, conduction times were prolonged in the suprahisian and mainly the infrahisian part and the atrial refractory period increased. These effects are related to quinidine-like properties and consistent with cardiotoxic effects previously reported in clinical practice.", "contents": "Study of thioridazine cardiotoxic effects by means of His bundle activity recording. The effect of thioridazine on sinusal automaticity, myocardial excitability and auriculoventricular conduction have been studied in dogs by measuring the spontaneous heart rate, the effective refractory period of the atrial contractile tissue, the time of auriculonodal and infrahisian conduction. Chloralose anaesthetized dogs were administered 10 mg/kg thioridazine hydrochloride via intravenous route. While sinusal automaticity was not altered, conduction times were prolonged in the suprahisian and mainly the infrahisian part and the atrial refractory period increased. These effects are related to quinidine-like properties and consistent with cardiotoxic effects previously reported in clinical practice."}
{"id": "PMID:474149", "title": "Supersensitivity of the rat urinary bladder following \"chemical sympathectomy\".", "content": "6-hydroxydopamine (100 mg/kg injected intravenously twice with a weekly interval) was used for the destruction of the adrenergic nerves in the rat detrusor muscle. Two to three weeks after the first injection of this substance the sensitivity of the detrusor muscle to parasympathomimetic and sympathomimetic drugs was tested by recording the intravesical pressure in situ. The sensitivity to methacholine was found to be unchanged, whereas a supersensitivity to noradrenaline, adrenaline, phenylephrine and isoprenaline was found to have developed, thus involving both alpha-adrenoceptor mediated contraction and beta-adrenoceptor mediated relaxation. The supersensitivity was of the postjunctional type.", "contents": "Supersensitivity of the rat urinary bladder following \"chemical sympathectomy\". 6-hydroxydopamine (100 mg/kg injected intravenously twice with a weekly interval) was used for the destruction of the adrenergic nerves in the rat detrusor muscle. Two to three weeks after the first injection of this substance the sensitivity of the detrusor muscle to parasympathomimetic and sympathomimetic drugs was tested by recording the intravesical pressure in situ. The sensitivity to methacholine was found to be unchanged, whereas a supersensitivity to noradrenaline, adrenaline, phenylephrine and isoprenaline was found to have developed, thus involving both alpha-adrenoceptor mediated contraction and beta-adrenoceptor mediated relaxation. The supersensitivity was of the postjunctional type."}
{"id": "PMID:474150", "title": "Sensitization of the submaxillary gland of the rat after sympathetic denervation.", "content": "The sensitivity of the submaxillary gland of the rat to chemical stimuli was examined 1, 4 and 6 weeks after sympathetic denervation. The gland was found to have developed a supersensitivity not only to noradrenaline but also, in contrast to previous reports, to adrenaline and methacholine. The supersensitivity remained unaltered throughout the period of observation.", "contents": "Sensitization of the submaxillary gland of the rat after sympathetic denervation. The sensitivity of the submaxillary gland of the rat to chemical stimuli was examined 1, 4 and 6 weeks after sympathetic denervation. The gland was found to have developed a supersensitivity not only to noradrenaline but also, in contrast to previous reports, to adrenaline and methacholine. The supersensitivity remained unaltered throughout the period of observation."}
{"id": "PMID:474151", "title": "Pharmacokinetics of dipyridamole.", "content": "Dipyridamole, though originally introduced as a coronary vasodilator, has lately been increasingly investigated in the treatment of thromboembolic diseases because of its inhibitory influence upon blood platelet function. The compound is eliminated from the organism by hepatic biotransformation to the monoglucuronide, which almost exclusively is subjected to biliary and faecal excretion with simultaneous partial enterohepatic circulation taking place. Only minute amounts are excreted through the kidneys. In experiments on four normal human volunteers it was found that the serum concentration curve after intravenous administration of dipyridamole rather closely fits the pharmacokinetics of an open two-compartment model with first order, linear disposition kinetics and elimination taking place from the central compartment. Experiments with oral ingestion of the compound could be described by the use of a corresponding pharmacokinetic model with two consecutive first order input steps, representing the dissolution and absorption processes. The disposition rate constants (beta) were within the range of 0.0051--0.0083 min.-1 corresponding to biological half-lives of 84--145 min. The absorption rate constant was about 0.07 min.-1, and the systemic availability of an oral dose of 100 mg dipyridamole in tablets varied from 37 to 66%.", "contents": "Pharmacokinetics of dipyridamole. Dipyridamole, though originally introduced as a coronary vasodilator, has lately been increasingly investigated in the treatment of thromboembolic diseases because of its inhibitory influence upon blood platelet function. The compound is eliminated from the organism by hepatic biotransformation to the monoglucuronide, which almost exclusively is subjected to biliary and faecal excretion with simultaneous partial enterohepatic circulation taking place. Only minute amounts are excreted through the kidneys. In experiments on four normal human volunteers it was found that the serum concentration curve after intravenous administration of dipyridamole rather closely fits the pharmacokinetics of an open two-compartment model with first order, linear disposition kinetics and elimination taking place from the central compartment. Experiments with oral ingestion of the compound could be described by the use of a corresponding pharmacokinetic model with two consecutive first order input steps, representing the dissolution and absorption processes. The disposition rate constants (beta) were within the range of 0.0051--0.0083 min.-1 corresponding to biological half-lives of 84--145 min. The absorption rate constant was about 0.07 min.-1, and the systemic availability of an oral dose of 100 mg dipyridamole in tablets varied from 37 to 66%."}
{"id": "PMID:474152", "title": "Effects of various in vitro--inhibitors of benzo(a)pyrene metabolism in isolated rat lung perfusion.", "content": "Various in vitro-inhibitors were added with 3H-benzo(a)pyrene (BP) into the perfusion fluids in isolated rat lung perfusions to see whether their effects are dependent on the integrity of tissue. 3H-BP and its metabolites were measured by thin-layer chromatography and radiometry from both samples of perfusion medium and homogenates of lung tissue. The total covalent binding to lung tissue was used as a measure of the formation of reactive metabolites. In methylcholanthrene-induced rat lung, the metabolism of BP was inhibited by alpha-naphthoflavone, an inhibitor of monooxygenase, and less with diethylmaleate, a depletor of glutathione, with salicylamide, an inhibitor of conjugases, and, astonishingly, with D-saccharo-1,4-lactone, an inhibitor of beta-glucuronidase. With trichloropropene oxide, which inhibits epoxide hydratase, the metabolism was either decreased or unchanged. Nicotine had no effect on BP-metabolism. Nicotine and diethylmaleate increased statistically significantly and alpha-naphthoflavone and salicylamide decreased the covalent binding of radioactivity to lung tissue. In most cases, the changes in BP metabolism observed during perfusion can be explained on the basis of effects of modifiers on the enzyme systems.", "contents": "Effects of various in vitro--inhibitors of benzo(a)pyrene metabolism in isolated rat lung perfusion. Various in vitro-inhibitors were added with 3H-benzo(a)pyrene (BP) into the perfusion fluids in isolated rat lung perfusions to see whether their effects are dependent on the integrity of tissue. 3H-BP and its metabolites were measured by thin-layer chromatography and radiometry from both samples of perfusion medium and homogenates of lung tissue. The total covalent binding to lung tissue was used as a measure of the formation of reactive metabolites. In methylcholanthrene-induced rat lung, the metabolism of BP was inhibited by alpha-naphthoflavone, an inhibitor of monooxygenase, and less with diethylmaleate, a depletor of glutathione, with salicylamide, an inhibitor of conjugases, and, astonishingly, with D-saccharo-1,4-lactone, an inhibitor of beta-glucuronidase. With trichloropropene oxide, which inhibits epoxide hydratase, the metabolism was either decreased or unchanged. Nicotine had no effect on BP-metabolism. Nicotine and diethylmaleate increased statistically significantly and alpha-naphthoflavone and salicylamide decreased the covalent binding of radioactivity to lung tissue. In most cases, the changes in BP metabolism observed during perfusion can be explained on the basis of effects of modifiers on the enzyme systems."}
{"id": "PMID:474153", "title": "Urinary elimination of nitrazepam and its main metabolites.", "content": "Nitrazepam and its main metabolites, 7-aminonitrazepam and 7-acetamidonitrazepam, free and conjugated, were determined in the human urine after a single oral dose of 5 mg. The determinations were performed by GLC method using 63Ni-EC-detector for unchanged nitrazepam and nitrogen selective detector for the metabolites. Unchanged nitrazepam was poorly eliminated through the kidneys (about 1% of the dose). The interindividual variation of total excreted urinary metabolites was large ranging between 848-4933 microgram (17-99% of the dose during 7 days). Of this amount conjugated metabolites made up 57%. The urinary half-lives of free and conjugated-7-aminonitrazepam were (mean and ranges) 44 (23-65) and 46 (25-69) hrs, and of 7-acetamidonitrazepam 12 (5-31) and 18 (5-46) hrs, respectively. The half-lives of the excreted amounts of the metabolites did not correlate with any pharmacokinetic parameter of unchanged nitrazepam in serum.", "contents": "Urinary elimination of nitrazepam and its main metabolites. Nitrazepam and its main metabolites, 7-aminonitrazepam and 7-acetamidonitrazepam, free and conjugated, were determined in the human urine after a single oral dose of 5 mg. The determinations were performed by GLC method using 63Ni-EC-detector for unchanged nitrazepam and nitrogen selective detector for the metabolites. Unchanged nitrazepam was poorly eliminated through the kidneys (about 1% of the dose). The interindividual variation of total excreted urinary metabolites was large ranging between 848-4933 microgram (17-99% of the dose during 7 days). Of this amount conjugated metabolites made up 57%. The urinary half-lives of free and conjugated-7-aminonitrazepam were (mean and ranges) 44 (23-65) and 46 (25-69) hrs, and of 7-acetamidonitrazepam 12 (5-31) and 18 (5-46) hrs, respectively. The half-lives of the excreted amounts of the metabolites did not correlate with any pharmacokinetic parameter of unchanged nitrazepam in serum."}
{"id": "PMID:474154", "title": "Pharmacokinetics of nitrazepam in saliva and serum after a single oral dose.", "content": "The pharmacokinetics of nitrazepam in saliva and serum was studied in 12 healthy volunteers after a single administration of a 5 mg nitrazepam tablet. The binding of nitrazepam to plasma proteins was determined 4 hours after the administration by ultracentrifugation. The analysis of nitrazepam concentrations was performed by 63Ni-EC-GLC. The pharmacokinetic parameters were evaluated manually or by AUTOAN-program in serum, and manually in saliva. The concentrations of nitrazepam in serum and saliva correlated significantly (r = 0.472, P less than 0.001, n = 97). The ratio saliva: serum was, however, time dependent. The protein free fraction in serum was significantly higher (P less than 0.01) than the salivary concentration at the same time (4 hours after administration). The peak concentrations in serum and saliva were 40.7 and 1.9 ng/ml (P less than 0.001) and the times to reach the peak maximum 2.4 and 2.5 hours, respectively (difference not significant). The mean half-life of nitrazepam in serum was 30.5 hrs and in saliva 39.9 hrs, the difference being significant at P less than 0.05. The distribution phase parameters, poorly described before, were calculated. The clinical value of nitrazepam analysis in saliva seems to be negligible.", "contents": "Pharmacokinetics of nitrazepam in saliva and serum after a single oral dose. The pharmacokinetics of nitrazepam in saliva and serum was studied in 12 healthy volunteers after a single administration of a 5 mg nitrazepam tablet. The binding of nitrazepam to plasma proteins was determined 4 hours after the administration by ultracentrifugation. The analysis of nitrazepam concentrations was performed by 63Ni-EC-GLC. The pharmacokinetic parameters were evaluated manually or by AUTOAN-program in serum, and manually in saliva. The concentrations of nitrazepam in serum and saliva correlated significantly (r = 0.472, P less than 0.001, n = 97). The ratio saliva: serum was, however, time dependent. The protein free fraction in serum was significantly higher (P less than 0.01) than the salivary concentration at the same time (4 hours after administration). The peak concentrations in serum and saliva were 40.7 and 1.9 ng/ml (P less than 0.001) and the times to reach the peak maximum 2.4 and 2.5 hours, respectively (difference not significant). The mean half-life of nitrazepam in serum was 30.5 hrs and in saliva 39.9 hrs, the difference being significant at P less than 0.05. The distribution phase parameters, poorly described before, were calculated. The clinical value of nitrazepam analysis in saliva seems to be negligible."}
{"id": "PMID:474155", "title": "Effects of digoxin on islated human mesenteric vessels.", "content": "Digoxin had contractant effects on isolated, human mesenteric arteries and veins. Veins were more sensitive to this action of the glycoside than arteries. The contractions were not affected by phentolamine or by washing with a digoxin-free solution. However, they were abolished by the calcium antagonist nifedipine, and by washing with a calcium-free medium. In the presence of digoxin, the maximum response to noradrenaline (1.8 x 10(-5) M) increased markedly in both arteries and veins, and the concentration-response curve for the amine was displaced to the left. Immersion of vein preparations in calcium-free solution for 30 min. abolished the digoxin contracture; an increase in extracellular calcium restored the response. Changes in extracellular potassium concentration caused changes in tension in tension of the mesenteric veins similar to those previously demonstrated in peripheral veins--both in the presence and in the absence of digoxin. It is concluded that digoxin contracts mesenteric vessels by a direct action on the muscle cells, and potentiates the contractant effects of noradrenaline. These effects are dependent on the availability of extracellular calcium. Mesenteric vascular reactions caused by changes in extracellular potassium are influenced by digoxin.", "contents": "Effects of digoxin on islated human mesenteric vessels. Digoxin had contractant effects on isolated, human mesenteric arteries and veins. Veins were more sensitive to this action of the glycoside than arteries. The contractions were not affected by phentolamine or by washing with a digoxin-free solution. However, they were abolished by the calcium antagonist nifedipine, and by washing with a calcium-free medium. In the presence of digoxin, the maximum response to noradrenaline (1.8 x 10(-5) M) increased markedly in both arteries and veins, and the concentration-response curve for the amine was displaced to the left. Immersion of vein preparations in calcium-free solution for 30 min. abolished the digoxin contracture; an increase in extracellular calcium restored the response. Changes in extracellular potassium concentration caused changes in tension in tension of the mesenteric veins similar to those previously demonstrated in peripheral veins--both in the presence and in the absence of digoxin. It is concluded that digoxin contracts mesenteric vessels by a direct action on the muscle cells, and potentiates the contractant effects of noradrenaline. These effects are dependent on the availability of extracellular calcium. Mesenteric vascular reactions caused by changes in extracellular potassium are influenced by digoxin."}
{"id": "PMID:474156", "title": "Ornithine decarboxylase activity and polyamine content of normal and fluoride resistant LS cells.", "content": "The activity of ornithine decarboxylase (ODC) in suspension cultures of mouse fibroblasts, LS cells, varied in a characteristic cyclic pattern after dilution of the cultures. Two strains of fluoride resistant LS cells, FR6 and LSFR6 cells, exhibited the same cyclic pattern, but with markedly higher ODC activities. These fluoride resistant cells, however, contained less putrescine, the product of the ODC reaction. Possible reasons for this finding are discussed.", "contents": "Ornithine decarboxylase activity and polyamine content of normal and fluoride resistant LS cells. The activity of ornithine decarboxylase (ODC) in suspension cultures of mouse fibroblasts, LS cells, varied in a characteristic cyclic pattern after dilution of the cultures. Two strains of fluoride resistant LS cells, FR6 and LSFR6 cells, exhibited the same cyclic pattern, but with markedly higher ODC activities. These fluoride resistant cells, however, contained less putrescine, the product of the ODC reaction. Possible reasons for this finding are discussed."}
{"id": "PMID:474157", "title": "Ascitic fluid modifying the distribution of sulphafurazole in rats with intestinal occlusion.", "content": "In order to confirm our previous findings of increased sulphafurazole (sulfisoxazole, SF) inactivation by intestinal occlusion, SF was given intravenously 20 mg/kg to rats with a low small intestinal occlusion and to sham-operated controls 40-45 hours after the operation. Occlusion did not cause major changes in the distribution of SF to various tissues, but there were some indications of increased SF acetylation after occlusion. The occlusion rats produced ascitic fluid into the abdominal cavity and the total SF levels in the ascitic fluid were almost identical to those in blood, also after oral administration of SF, 50 mg/kg. After intraperitoneal administration of 2 ml of SF (60 microgram/ml) more SF accumulated into the small intestinal wall in occlusion rats than in controls, and the acetyl SF levels were increased in the small and large intestine. So, our previous suggestion of an increased excretory function of the large intestine in occlusion states may not be the only explanation for increased drug levels in the large intestine found after oral administration of SF. Part of the SF in the intestinal wall can also result from ascitic fluid SF, and ascites must be taken into account when considering drug pharmacokinetics.", "contents": "Ascitic fluid modifying the distribution of sulphafurazole in rats with intestinal occlusion. In order to confirm our previous findings of increased sulphafurazole (sulfisoxazole, SF) inactivation by intestinal occlusion, SF was given intravenously 20 mg/kg to rats with a low small intestinal occlusion and to sham-operated controls 40-45 hours after the operation. Occlusion did not cause major changes in the distribution of SF to various tissues, but there were some indications of increased SF acetylation after occlusion. The occlusion rats produced ascitic fluid into the abdominal cavity and the total SF levels in the ascitic fluid were almost identical to those in blood, also after oral administration of SF, 50 mg/kg. After intraperitoneal administration of 2 ml of SF (60 microgram/ml) more SF accumulated into the small intestinal wall in occlusion rats than in controls, and the acetyl SF levels were increased in the small and large intestine. So, our previous suggestion of an increased excretory function of the large intestine in occlusion states may not be the only explanation for increased drug levels in the large intestine found after oral administration of SF. Part of the SF in the intestinal wall can also result from ascitic fluid SF, and ascites must be taken into account when considering drug pharmacokinetics."}
{"id": "PMID:474158", "title": "Glutathione depletion in isolated hepatocytes: its relation to lipid peroxidation and cell damage.", "content": "The effects of glutathione depletion in isolated hepatocytes have been studied. A list of compounds which depleted glutathione and induced lipid peroxidation and cell lysis is given. The effects of halogenated acetamides were studied in more detail and results of studies on the interaction of iodoacetamide with cellular constituents are presented. A single metabolite of iodoacetamide, tentatively identified as the glutathione conjugate, was excreted from the cells while less than one percent of the \"parent compound\" was retained, tightly bound to macromolecules. This bound component could not be associated with the cellular damage. Methionine, cysteine and alpha-tocopherol, as wellas paracetamol and ethylmorphine, were found to prevent lipid peroxidation and lysis. It is concluded that GSH deficiency per se can lead to lipid peroxidation and that this reaction caused the observed hepatocellular lysis.", "contents": "Glutathione depletion in isolated hepatocytes: its relation to lipid peroxidation and cell damage. The effects of glutathione depletion in isolated hepatocytes have been studied. A list of compounds which depleted glutathione and induced lipid peroxidation and cell lysis is given. The effects of halogenated acetamides were studied in more detail and results of studies on the interaction of iodoacetamide with cellular constituents are presented. A single metabolite of iodoacetamide, tentatively identified as the glutathione conjugate, was excreted from the cells while less than one percent of the \"parent compound\" was retained, tightly bound to macromolecules. This bound component could not be associated with the cellular damage. Methionine, cysteine and alpha-tocopherol, as wellas paracetamol and ethylmorphine, were found to prevent lipid peroxidation and lysis. It is concluded that GSH deficiency per se can lead to lipid peroxidation and that this reaction caused the observed hepatocellular lysis."}
{"id": "PMID:474159", "title": "Sotalol intoxication, two patients with concentration-effect relationships.", "content": "Two adult patients ingested an overdose of 2.4 g and 8.0 g of sotalol hydrochloride, respectively, i.e. 7-25 times the mean daily dose. Certain signs as bradycardia and hypotension were similar to those described for other beta-blocking agents. In addition to these, however, both patients had severe cardiac tachyarrhythmias and a considerably prolonged QT-interval in their electrocardiogram. The decline of serum sotalol concentrations followed first-order kinetics with the elimination half-life of 13-15 hours. There was a good correlation between the serum sotalol concentration and the prolongation of the QT-interval. Sotalol differs from other beta-blocking agents in its effects on the action potential of the ventricular muscle and Purkinje fibers of the heart. This is likely to explain the different symptoms and findings of sotalol intoxication compared to those seen in connection with other beta-blocking agents.", "contents": "Sotalol intoxication, two patients with concentration-effect relationships. Two adult patients ingested an overdose of 2.4 g and 8.0 g of sotalol hydrochloride, respectively, i.e. 7-25 times the mean daily dose. Certain signs as bradycardia and hypotension were similar to those described for other beta-blocking agents. In addition to these, however, both patients had severe cardiac tachyarrhythmias and a considerably prolonged QT-interval in their electrocardiogram. The decline of serum sotalol concentrations followed first-order kinetics with the elimination half-life of 13-15 hours. There was a good correlation between the serum sotalol concentration and the prolongation of the QT-interval. Sotalol differs from other beta-blocking agents in its effects on the action potential of the ventricular muscle and Purkinje fibers of the heart. This is likely to explain the different symptoms and findings of sotalol intoxication compared to those seen in connection with other beta-blocking agents."}
{"id": "PMID:474160", "title": "Penetration enhancers and other factors governing percutaneous local anaesthesia with lidocaine.", "content": "The percutaneous penetration of the local anaestetic lidocaine was investigated in the guinea-pig. Three different types of composition were employed: lidocaine hydrochloride in aqueous solution, lidocaine base in an aqueous alcoholic solvent mixture and lidocaine base in aqueous solutions of dipolar aprotic solvents. The latter solvents included simple tertiary aliphatic amides, amides related to dimethylacetamide, some cyclic amides as well as a number of miscellaneous compounds. The degree of dermal anaesthesia was noted in each case. In addition, the uptake and distribution of lidocaine in the skin and its absorption into the blood were studied using tritium-labelled drug. The results show that the percutaneous penetration of lidocaine is dependent on the concentration of the agent, the time of epicutaneous application of the composition, whether the agent was used as salt or free base, and the nature of the solvent medium. Lidocaine base in aqueous dimethylacetamide was most effective in producing percutaneous local anaesthesia.", "contents": "Penetration enhancers and other factors governing percutaneous local anaesthesia with lidocaine. The percutaneous penetration of the local anaestetic lidocaine was investigated in the guinea-pig. Three different types of composition were employed: lidocaine hydrochloride in aqueous solution, lidocaine base in an aqueous alcoholic solvent mixture and lidocaine base in aqueous solutions of dipolar aprotic solvents. The latter solvents included simple tertiary aliphatic amides, amides related to dimethylacetamide, some cyclic amides as well as a number of miscellaneous compounds. The degree of dermal anaesthesia was noted in each case. In addition, the uptake and distribution of lidocaine in the skin and its absorption into the blood were studied using tritium-labelled drug. The results show that the percutaneous penetration of lidocaine is dependent on the concentration of the agent, the time of epicutaneous application of the composition, whether the agent was used as salt or free base, and the nature of the solvent medium. Lidocaine base in aqueous dimethylacetamide was most effective in producing percutaneous local anaesthesia."}
{"id": "PMID:474161", "title": "Inter and intra laboratory variation of digoxin radioimmunoassay in Sweden.", "content": "Samples from two pools were sent 10 times to 27 laboratories for assay of digoxin. One pool contained digoxin 2.60 nmol/l in normal plasma (SP); the other was pooled plasma from patients treated with digoxin (PP). Ten radioimmunoassay (RIA) methods were used. The mean of SP assays was 2.59 nmol/l, not significantly different from 2.60 nmol/l. The mean of PP determinations was 2.46 nmol/l. Within each of the 10 assay rounds, the concentrations showed an almost twofold variation and S.D. averaged 0.33 nmol/l and 0.31 nmol/l for SP and PP respectively. Significant differences (P less than 0.001) were found between mean concentrations obtained for the pools at various laboratories (SP range 2.15-2.85 nmol/l; PP range 2.12-2.72 nmol/l). The laboratory means obtained for SP and PP correlated significantly (P less than 0.001). Nevertheless, significant (P less than 0.01) variations between laboratories were found also concerning the mean difference between SP and PP concentrations. The interassay SD of the assays differed significantly between laboratories (range 0.05-0.61 nmol/l. Between and within groups of laboratories using the same RIA method and between various types of laboratories, differences were also found concerning both accuracy and precision of the assays. It is concluded that a better control of digoxin assay is needed.", "contents": "Inter and intra laboratory variation of digoxin radioimmunoassay in Sweden. Samples from two pools were sent 10 times to 27 laboratories for assay of digoxin. One pool contained digoxin 2.60 nmol/l in normal plasma (SP); the other was pooled plasma from patients treated with digoxin (PP). Ten radioimmunoassay (RIA) methods were used. The mean of SP assays was 2.59 nmol/l, not significantly different from 2.60 nmol/l. The mean of PP determinations was 2.46 nmol/l. Within each of the 10 assay rounds, the concentrations showed an almost twofold variation and S.D. averaged 0.33 nmol/l and 0.31 nmol/l for SP and PP respectively. Significant differences (P less than 0.001) were found between mean concentrations obtained for the pools at various laboratories (SP range 2.15-2.85 nmol/l; PP range 2.12-2.72 nmol/l). The laboratory means obtained for SP and PP correlated significantly (P less than 0.001). Nevertheless, significant (P less than 0.01) variations between laboratories were found also concerning the mean difference between SP and PP concentrations. The interassay SD of the assays differed significantly between laboratories (range 0.05-0.61 nmol/l. Between and within groups of laboratories using the same RIA method and between various types of laboratories, differences were also found concerning both accuracy and precision of the assays. It is concluded that a better control of digoxin assay is needed."}
{"id": "PMID:474162", "title": "Studies on the distribution and metabolism of 14C-dimethylnitrosamine in foetal and young mice.", "content": "In pregnant mice injected with 14C-dimethylnitrosamine, whole-body autoradiography was performed with hemisections at -80 degrees (to prevent evaporation of the volatile dimethylnitrosamine) and with dry tape sections (to localize the non-volatile metabolites). The results indicated that the non-metabolized substance passed to the foetal tissues with a uniform distribution and without formation or accumulation of non-volatile metabolites. Autoradiography in young (1-10 days old) and adult mice showed a high level of metabolites in the liver already 5 min. after the administration of 14C-dimethylnitrosamine. No metabolism of the substance could be detected at in vitro incubations of liver tissue obtained from foetuses on the last day of gestation (14CO2-production and incorporation of radioactivity in acid-insoluble macromolecules were used as metabolic indices). However, in vitro experiments with livers of 1-5 days old mice indicated a rapid increase in enzymatic activity after birth. Studies in vivo showed an increased incorporation of radioactivity in the acid-insoluble macromolecules of the liver and a decreased exhalation of 14CO2 in 10 and 14 days old mice as compared with 21 and 60 days old mice. This indicates a difference in the fate of dimethylnitrosamine in vivo between the young and older mice.", "contents": "Studies on the distribution and metabolism of 14C-dimethylnitrosamine in foetal and young mice. In pregnant mice injected with 14C-dimethylnitrosamine, whole-body autoradiography was performed with hemisections at -80 degrees (to prevent evaporation of the volatile dimethylnitrosamine) and with dry tape sections (to localize the non-volatile metabolites). The results indicated that the non-metabolized substance passed to the foetal tissues with a uniform distribution and without formation or accumulation of non-volatile metabolites. Autoradiography in young (1-10 days old) and adult mice showed a high level of metabolites in the liver already 5 min. after the administration of 14C-dimethylnitrosamine. No metabolism of the substance could be detected at in vitro incubations of liver tissue obtained from foetuses on the last day of gestation (14CO2-production and incorporation of radioactivity in acid-insoluble macromolecules were used as metabolic indices). However, in vitro experiments with livers of 1-5 days old mice indicated a rapid increase in enzymatic activity after birth. Studies in vivo showed an increased incorporation of radioactivity in the acid-insoluble macromolecules of the liver and a decreased exhalation of 14CO2 in 10 and 14 days old mice as compared with 21 and 60 days old mice. This indicates a difference in the fate of dimethylnitrosamine in vivo between the young and older mice."}
{"id": "PMID:474170", "title": "Bimodal distribution of serum trypotphan level.", "content": "Serum levels of total L-tryptophan (L-TP) were determined before and during treatment in patients suffering from endogenous depression, who were treated with L-TP, 6 g daily, and unilateral electroconvulsive therapy (ECT). The gaussian curve of initial L-TP concentrations changed into a bimodal distribution during treatment. The patients with initially lower L-TP levels also had lower L-TP levels during the treatment, thus forming the low-concentration group within the bimodal distribution. In the patients with higher initial concentrations, the L-TP levels increased to a much greater degree, forming the high-concentration group. Patients in this group needed a larger number of ECT's, probably owing to L-TP's property of shortening the seizure duration. No other background data or clinical variables showed significant differences between the groups. It is suggested that the bimodality of L-TP levels is genetically determined.", "contents": "Bimodal distribution of serum trypotphan level. Serum levels of total L-tryptophan (L-TP) were determined before and during treatment in patients suffering from endogenous depression, who were treated with L-TP, 6 g daily, and unilateral electroconvulsive therapy (ECT). The gaussian curve of initial L-TP concentrations changed into a bimodal distribution during treatment. The patients with initially lower L-TP levels also had lower L-TP levels during the treatment, thus forming the low-concentration group within the bimodal distribution. In the patients with higher initial concentrations, the L-TP levels increased to a much greater degree, forming the high-concentration group. Patients in this group needed a larger number of ECT's, probably owing to L-TP's property of shortening the seizure duration. No other background data or clinical variables showed significant differences between the groups. It is suggested that the bimodality of L-TP levels is genetically determined."}
{"id": "PMID:474171", "title": "Psychiatric illness among adults adopted as infants.", "content": "In an epidemiological study of 2,323 adopted adults, the registrations about sick-leave, diagnoses and hospitalization (F-cards) from the National Health Insurance were studied. There was a significantly higher frequency of psychiatric illness among the adoptees compared with non-adopted controls. A classification into different diagnostic groups from the records of psychiatric hospitals showed an over-representation due to alcohol and drug abuse and personality disorders in both male and female adoptee groups compared with non-adopted controls. There was also an over-representation in the male adoptee group due to neuroses.", "contents": "Psychiatric illness among adults adopted as infants. In an epidemiological study of 2,323 adopted adults, the registrations about sick-leave, diagnoses and hospitalization (F-cards) from the National Health Insurance were studied. There was a significantly higher frequency of psychiatric illness among the adoptees compared with non-adopted controls. A classification into different diagnostic groups from the records of psychiatric hospitals showed an over-representation due to alcohol and drug abuse and personality disorders in both male and female adoptee groups compared with non-adopted controls. There was also an over-representation in the male adoptee group due to neuroses."}
{"id": "PMID:474172", "title": "Parental contact in male and female transsexuals.", "content": "The parental contact reported by 12 biologically male and 12 biologically female transsexuals was compared with that reported by control subjects (109 conscripts in the Swedish Army; 101 pregnant nulliparous women). Parental contact was estimated for three different periods: 1) childhood, 2) adolescence, 3) the year before investigation. Transsexual patients generally reported less satisfactory relations to both parents than same-sexed control subjects. A pattern characterized by warm and intense contact with the cross-sexed parent and poor contact with the same-sexed one was observed in about half of the male transsexuals, but not in the female ones. Our findings suggest that disturbed emotional relations to the parents may be one contributory cause in the development of transsexualism and are in accordance with the hypothesis that gender identity grows out of an interaction of biological and environmental factors.", "contents": "Parental contact in male and female transsexuals. The parental contact reported by 12 biologically male and 12 biologically female transsexuals was compared with that reported by control subjects (109 conscripts in the Swedish Army; 101 pregnant nulliparous women). Parental contact was estimated for three different periods: 1) childhood, 2) adolescence, 3) the year before investigation. Transsexual patients generally reported less satisfactory relations to both parents than same-sexed control subjects. A pattern characterized by warm and intense contact with the cross-sexed parent and poor contact with the same-sexed one was observed in about half of the male transsexuals, but not in the female ones. Our findings suggest that disturbed emotional relations to the parents may be one contributory cause in the development of transsexualism and are in accordance with the hypothesis that gender identity grows out of an interaction of biological and environmental factors."}
{"id": "PMID:474173", "title": "Family-size and birth-order in obsessional neurosis.", "content": "Family size and birth-order of 156 British-born patients diagnosed as having obsessive-compulsive neurosis were determined from case-notes; these were compared with matched controls. Separate consideration was given to male and female patients. Mean family size of obsessionals was found to be smaller than that of controls. An excess of first-born was found among male obsessional patients.", "contents": "Family-size and birth-order in obsessional neurosis. Family size and birth-order of 156 British-born patients diagnosed as having obsessive-compulsive neurosis were determined from case-notes; these were compared with matched controls. Separate consideration was given to male and female patients. Mean family size of obsessionals was found to be smaller than that of controls. An excess of first-born was found among male obsessional patients."}
{"id": "PMID:474174", "title": "Monoamines and monoamine metabolites in brains from demented schizophrenics.", "content": "The brains from 12 schizophrenic patients were investigated post-mortem for their content of noradrenaline (NA), dopamine (DA), 5-hydroxytryptamine (5-HT), homovanillic acid (HVA), and 5-hydroxyindolacetic acid (5-HIAA). Six of the schizophrenics had been lobotomized 25--30 years prior to death. A control group matched for age was collected in the autopsy room. The concentrations of NA, DA, and HVA in different parts of the brain from the schizophrenic group did not differ from those of the controls. 5-HT was determined in 11 nuclei or areas of the brain. The schizophrenic group had lower mean values compared with the controls, and in the hypothalamus, medulla oblongata, and hippocampus the difference was at a significant level. 5-HIAA was determined in six areas of the brain but only in a few cases. There was a trend towards lower means of 5-HIAA in the schizophrenics. Cause of death, medication, food intake, age, time between death and autopsy, time the corpses have lain in room temperature, and dissection technique are discussed in relation to these findings. These variables have to be kept under careful control before changes can be claimed as having pathogenetic importance for schizophrenia or for the progressing dementia in this disease.", "contents": "Monoamines and monoamine metabolites in brains from demented schizophrenics. The brains from 12 schizophrenic patients were investigated post-mortem for their content of noradrenaline (NA), dopamine (DA), 5-hydroxytryptamine (5-HT), homovanillic acid (HVA), and 5-hydroxyindolacetic acid (5-HIAA). Six of the schizophrenics had been lobotomized 25--30 years prior to death. A control group matched for age was collected in the autopsy room. The concentrations of NA, DA, and HVA in different parts of the brain from the schizophrenic group did not differ from those of the controls. 5-HT was determined in 11 nuclei or areas of the brain. The schizophrenic group had lower mean values compared with the controls, and in the hypothalamus, medulla oblongata, and hippocampus the difference was at a significant level. 5-HIAA was determined in six areas of the brain but only in a few cases. There was a trend towards lower means of 5-HIAA in the schizophrenics. Cause of death, medication, food intake, age, time between death and autopsy, time the corpses have lain in room temperature, and dissection technique are discussed in relation to these findings. These variables have to be kept under careful control before changes can be claimed as having pathogenetic importance for schizophrenia or for the progressing dementia in this disease."}
{"id": "PMID:474175", "title": "Dissimulation function in some functional psychoses.", "content": "Dissimulation function as estimated by means of Lie-Scale scores can be used as a pointer towards the impaired self-appraising ability of the individuals in relation to others. Sixty non-psychiatric normal persons and 133 psychiatric cases, representing two major psychotic groups, were investigated for the dissimulation function. The results show a significant difference between the two groups of normal and abnormal individuals, the abnormal group dissimulating distinctively more than the other. However, this impaired self-appraising ability does not seem to relate to the chronicity of the illness but only partly with the typology of illness.", "contents": "Dissimulation function in some functional psychoses. Dissimulation function as estimated by means of Lie-Scale scores can be used as a pointer towards the impaired self-appraising ability of the individuals in relation to others. Sixty non-psychiatric normal persons and 133 psychiatric cases, representing two major psychotic groups, were investigated for the dissimulation function. The results show a significant difference between the two groups of normal and abnormal individuals, the abnormal group dissimulating distinctively more than the other. However, this impaired self-appraising ability does not seem to relate to the chronicity of the illness but only partly with the typology of illness."}
{"id": "PMID:474176", "title": "Neurasthenic symptoms in workers occupationally exposed to jet fuel.", "content": "Long-term exposure to petroleum distillation products (e.g. jet fuel) has been suggested to cause chronic mental and neurological symptoms. In the present \"cross-sectional epidemiological study\" the extent of neuropsychiatric ill-health in 30 workers exposed to jet fuel was compared with that in 60 non-exposed matched controls. Standardized medical interviews showed a higher occurrence of neurasthenic symptoms in exposed subjects (P less than 0.001). The result was confirmed by examination of the medical records kept by the factory physician (P less than 0.01). The prevalence of psychiatric symptoms was assessed with a rating scale of 37 items (a modified CPRS). Again, the exposed workers scored higher than the controls (P less than 0.001), particularly regarding the neurasthenic symptoms, i.e. fatigue, anxiety, mood changes, memory difficulties, and various psychosomatic symptoms (P less than 0.01). The results could neither be explained by observer's bias, nor by a greater susceptibility for mental disorders in the exposed subjects. We therefore conclude that occupational exposure to jet fuel vapours around the present threshold limit values may induce a neurasthenic syndrome.", "contents": "Neurasthenic symptoms in workers occupationally exposed to jet fuel. Long-term exposure to petroleum distillation products (e.g. jet fuel) has been suggested to cause chronic mental and neurological symptoms. In the present \"cross-sectional epidemiological study\" the extent of neuropsychiatric ill-health in 30 workers exposed to jet fuel was compared with that in 60 non-exposed matched controls. Standardized medical interviews showed a higher occurrence of neurasthenic symptoms in exposed subjects (P less than 0.001). The result was confirmed by examination of the medical records kept by the factory physician (P less than 0.01). The prevalence of psychiatric symptoms was assessed with a rating scale of 37 items (a modified CPRS). Again, the exposed workers scored higher than the controls (P less than 0.001), particularly regarding the neurasthenic symptoms, i.e. fatigue, anxiety, mood changes, memory difficulties, and various psychosomatic symptoms (P less than 0.01). The results could neither be explained by observer's bias, nor by a greater susceptibility for mental disorders in the exposed subjects. We therefore conclude that occupational exposure to jet fuel vapours around the present threshold limit values may induce a neurasthenic syndrome."}
{"id": "PMID:474177", "title": "Validity of some neuropsychological tests in the assessment of mental deterioration.", "content": "Eighty-three control subjects and 200 patients suspected of diffuse cerebral damage were submitted to the Mental Deterioration Battery. Three independent judges evaluated each patient on the basis of all available data. Only 103 patients, unanimously classed as demented by the three judges were retained. On the basis of controls' performances, the scores obtained by each demented patient in the various tests were corrected for age and educational level. Both single tests and the Battery as a whole discriminated normal controls from demented patients at a very satisfactory level.", "contents": "Validity of some neuropsychological tests in the assessment of mental deterioration. Eighty-three control subjects and 200 patients suspected of diffuse cerebral damage were submitted to the Mental Deterioration Battery. Three independent judges evaluated each patient on the basis of all available data. Only 103 patients, unanimously classed as demented by the three judges were retained. On the basis of controls' performances, the scores obtained by each demented patient in the various tests were corrected for age and educational level. Both single tests and the Battery as a whole discriminated normal controls from demented patients at a very satisfactory level."}
{"id": "PMID:474178", "title": "Intra- and interhemispheric EEG differences quantified by spectral analysis. Comparative study of two groups of schizophrenics and a control group.", "content": "In this work we have studied comparatively the relationships between alpha intensity, alpha frequency, root-mean-square amplitude and their standard deviations, versus the location of the four recorded EEG channels: left and right rolando-parietal, left and right parieto-occipital. Twenty-four spectral parameters were computed for each 5-min EEG recording, in the eyes closed situation. The hebephrenic group (36 EEGs from six patients) presented higher values of alpha intensity and RMS amplitudes with low coefficient of variation when compared with the other groups. The left/right rolando-parietal amplitude ratio was 0.73 versus 1.52 for the parieto-occipital ratio. An amplitude hypovariability in the central regions, probably associated with hyperarousal, especially in the left hemisphere, can then be observed in hebephrenics, together with high alpha intensity on the left parieto-occipital area. The two groups of schizophrenics differ from the control group (27 EEGs from 12 subjects) in terms of spectral parameters, the hebephrenic group presenting a possible interhemispheric dysfunction.", "contents": "Intra- and interhemispheric EEG differences quantified by spectral analysis. Comparative study of two groups of schizophrenics and a control group. In this work we have studied comparatively the relationships between alpha intensity, alpha frequency, root-mean-square amplitude and their standard deviations, versus the location of the four recorded EEG channels: left and right rolando-parietal, left and right parieto-occipital. Twenty-four spectral parameters were computed for each 5-min EEG recording, in the eyes closed situation. The hebephrenic group (36 EEGs from six patients) presented higher values of alpha intensity and RMS amplitudes with low coefficient of variation when compared with the other groups. The left/right rolando-parietal amplitude ratio was 0.73 versus 1.52 for the parieto-occipital ratio. An amplitude hypovariability in the central regions, probably associated with hyperarousal, especially in the left hemisphere, can then be observed in hebephrenics, together with high alpha intensity on the left parieto-occipital area. The two groups of schizophrenics differ from the control group (27 EEGs from 12 subjects) in terms of spectral parameters, the hebephrenic group presenting a possible interhemispheric dysfunction."}
{"id": "PMID:474179", "title": "Attempted suicide. An Egyptian investigation.", "content": "This study comprises 200 cases from a total of 1,155 patients who attempted suicide in 1975 and were admitted to the casualty department of Ain Shams University Hospital in Cairo, which has a catchment area comprising approximately 3 million people. A crude rate of suicide attempts in Cairo would be 38.5/100,000. There was a high percentage among the age group 15--44 years with no major differences between the two sexes. Single patients represented 53% of the total, with students showing the highest risk (40%). Depressive illnesses, hysterical reactions, and situational disorders, in that order of frequency, were the main causes of the attempt. Overdose by tablet ingestion was the most common method used (80%). Official government records are misleading and do not represent the true rate.", "contents": "Attempted suicide. An Egyptian investigation. This study comprises 200 cases from a total of 1,155 patients who attempted suicide in 1975 and were admitted to the casualty department of Ain Shams University Hospital in Cairo, which has a catchment area comprising approximately 3 million people. A crude rate of suicide attempts in Cairo would be 38.5/100,000. There was a high percentage among the age group 15--44 years with no major differences between the two sexes. Single patients represented 53% of the total, with students showing the highest risk (40%). Depressive illnesses, hysterical reactions, and situational disorders, in that order of frequency, were the main causes of the attempt. Overdose by tablet ingestion was the most common method used (80%). Official government records are misleading and do not represent the true rate."}
{"id": "PMID:474181", "title": "Angiotensin II in primary hypertension, relationship to plasma renin activity, aldosterone and urinary electrolytes.", "content": "Plasma concentrations of angiotensin II (AII) were studied in 36 patients with primary (essential) hypertension and 15 normotensive control subjects during basal (1 h supine rest), upright and frusemide-stimulated (80 mg orally) conditions. Plasma renin activity (PRA) and plasma aldosterone (PA) were determined on the same occasions. AII was then correlated statistically to PRA, PA and 24-hour urinary excretions of aldosterone (Aldo-U), sodium and potassium and to the blood pressure (BP) levels. The AII values in the hypertensive patients were not statistically significantly different from those in the normotensive subjects. A close relationship was found between the AII values and the corresponding PRA values in the hypertensive patients (r=0.65--0.76, p less than 0.001 for all). Correlations between AII and PA, and AII and Aldo-U were not consistently significant. No correlation was found between AII and BP or between AII and 24-hour urinary electrolytes. The findings point to an intact function between PRA and AII but a disturbed AII-aldosterone interrelation in primary hypertension.", "contents": "Angiotensin II in primary hypertension, relationship to plasma renin activity, aldosterone and urinary electrolytes. Plasma concentrations of angiotensin II (AII) were studied in 36 patients with primary (essential) hypertension and 15 normotensive control subjects during basal (1 h supine rest), upright and frusemide-stimulated (80 mg orally) conditions. Plasma renin activity (PRA) and plasma aldosterone (PA) were determined on the same occasions. AII was then correlated statistically to PRA, PA and 24-hour urinary excretions of aldosterone (Aldo-U), sodium and potassium and to the blood pressure (BP) levels. The AII values in the hypertensive patients were not statistically significantly different from those in the normotensive subjects. A close relationship was found between the AII values and the corresponding PRA values in the hypertensive patients (r=0.65--0.76, p less than 0.001 for all). Correlations between AII and PA, and AII and Aldo-U were not consistently significant. No correlation was found between AII and BP or between AII and 24-hour urinary electrolytes. The findings point to an intact function between PRA and AII but a disturbed AII-aldosterone interrelation in primary hypertension."}
{"id": "PMID:474182", "title": "Heart and kidney involvement and prognosis in hypertension. A study concerning referred hypertensive patients and hypertensive patients found by blood pressure screening.", "content": "Severity of hypertension, frequency of secondary hypertension and prognosis have been compared in two groups of hypertensive men. The first group (n=686) was taken from a blood pressure screening of a total population sample. The other group (n=154) consisted of hypertensive men, referred to a hypertension clinic by physicians. The mean age of the groups was the same, (X=52 years, range 46--59 years). All went through the same investigations and were followed up and treated in a similar way at the hypertension clinic. The referred men had more severe hypertension, as shown by significantly more heart and kidney involvements. They also had a higher incidence of myocardial infarction, implying a poorer prognosis with regard to cardiovascular disease. The analysis shows the importance of a detailed description of studied groups, not only in terms of blood pressure, age and sex, but also with respect to the frequency and degree of present and previous signs of heart and kidney involvement. With such a description it is possible to compare results from different studies regarding pathophysiological mechanisms and the effect of treatment in hypertension.", "contents": "Heart and kidney involvement and prognosis in hypertension. A study concerning referred hypertensive patients and hypertensive patients found by blood pressure screening. Severity of hypertension, frequency of secondary hypertension and prognosis have been compared in two groups of hypertensive men. The first group (n=686) was taken from a blood pressure screening of a total population sample. The other group (n=154) consisted of hypertensive men, referred to a hypertension clinic by physicians. The mean age of the groups was the same, (X=52 years, range 46--59 years). All went through the same investigations and were followed up and treated in a similar way at the hypertension clinic. The referred men had more severe hypertension, as shown by significantly more heart and kidney involvements. They also had a higher incidence of myocardial infarction, implying a poorer prognosis with regard to cardiovascular disease. The analysis shows the importance of a detailed description of studied groups, not only in terms of blood pressure, age and sex, but also with respect to the frequency and degree of present and previous signs of heart and kidney involvement. With such a description it is possible to compare results from different studies regarding pathophysiological mechanisms and the effect of treatment in hypertension."}
{"id": "PMID:474183", "title": "A follow-up study of hypertensive patients after operative treatment of unilateral renovascular or renal disease.", "content": "A study of 44 hypertensive patients with unilateral renovascular or renal parenchymal disease is presented. All patients underwent corrective surgery. Out of the 44 operated patients, five did not participate in the follow-up examination. The remaining 39 patients constitute the study population. The effects of surgery on the hypertensive state could be evaluated in 35 patients, whereas four died less than two months after the operation. Follow-up studies were carried out at 8-60 months after the operation. The average period of observation was 32 months; 24 patients were observed for more than two years. As a group, the patients had severe hypertension with extensive target organ damage and widespread atherosclerosis. A fairly rigorous selection process was applied, and an unsatisfactory response to medical management was considered a point of major importance. In the majority of cases, renovascular lesions were atherosclerotic, with only two cases of fibromuscular dysplasia. Unilateral nephrectomy was performed in 32 patients, whereas seven underwent reconstructive vascular surgery. Out of 35 patients, 22 (63%) were cured, 8 (23%) improved and 5 (14%) unaltered. A gratifying regression of hypertensive lesions in target organs was observed in patients who were cured or improved by surgery. The frequency and severity of postoperative complications were related to the presence of extrarenal vascular disease.", "contents": "A follow-up study of hypertensive patients after operative treatment of unilateral renovascular or renal disease. A study of 44 hypertensive patients with unilateral renovascular or renal parenchymal disease is presented. All patients underwent corrective surgery. Out of the 44 operated patients, five did not participate in the follow-up examination. The remaining 39 patients constitute the study population. The effects of surgery on the hypertensive state could be evaluated in 35 patients, whereas four died less than two months after the operation. Follow-up studies were carried out at 8-60 months after the operation. The average period of observation was 32 months; 24 patients were observed for more than two years. As a group, the patients had severe hypertension with extensive target organ damage and widespread atherosclerosis. A fairly rigorous selection process was applied, and an unsatisfactory response to medical management was considered a point of major importance. In the majority of cases, renovascular lesions were atherosclerotic, with only two cases of fibromuscular dysplasia. Unilateral nephrectomy was performed in 32 patients, whereas seven underwent reconstructive vascular surgery. Out of 35 patients, 22 (63%) were cured, 8 (23%) improved and 5 (14%) unaltered. A gratifying regression of hypertensive lesions in target organs was observed in patients who were cured or improved by surgery. The frequency and severity of postoperative complications were related to the presence of extrarenal vascular disease."}
{"id": "PMID:474184", "title": "Glomerulonephritis and exposure to organic solvents. A case-control study.", "content": "Fifty patients with biopsy-proven glomerulonephritis and 100 sex- and age-matched controls (50 patients each with non-glomerular renal disease or acute appendicitis) were asked by questionnaire and a telephone interview whether they had been exposed to organic solvents. The questioning and the evaluation of the exposure, if any, was made without knowing the diagnosis of the interviewee. Fifty per cent of the patients with glomerulonephritis reported more than slight exposure, but only 20% of the controls. Exposure to organic solvents may often play a role in the causation of glomerulonephritis.", "contents": "Glomerulonephritis and exposure to organic solvents. A case-control study. Fifty patients with biopsy-proven glomerulonephritis and 100 sex- and age-matched controls (50 patients each with non-glomerular renal disease or acute appendicitis) were asked by questionnaire and a telephone interview whether they had been exposed to organic solvents. The questioning and the evaluation of the exposure, if any, was made without knowing the diagnosis of the interviewee. Fifty per cent of the patients with glomerulonephritis reported more than slight exposure, but only 20% of the controls. Exposure to organic solvents may often play a role in the causation of glomerulonephritis."}
{"id": "PMID:474185", "title": "Acute glomerulonephritis and exposure to organic solvents in father and daughter.", "content": "A man and his daughter had acute glomerulonephritis, the daughter eight years after the father. Both were exposed to vapours of organic solvents shortly before the onset of nephritis.", "contents": "Acute glomerulonephritis and exposure to organic solvents in father and daughter. A man and his daughter had acute glomerulonephritis, the daughter eight years after the father. Both were exposed to vapours of organic solvents shortly before the onset of nephritis."}
{"id": "PMID:474186", "title": "Cellular immunity in renal diseases.", "content": "Cellular immune reactivity was studied in 78 patients with various forms of renal disease by skin testing with four recall antigens and a lymphocyte transformation test with tuberculin PPD and leucoagglutinin. Patients with S-creatine greater than or equal to 230 micromol/l as well as those with chronic pyelonephritis who had S-creatinine values below 230 micromol/l had significantly lower skin reactions than the controls to streptokinase-streptodornase, parotitis and PPD. Glomerulonephritic patients with S-creatinine values below 230 micromol/l had normal skin reactivity. Lymphocyte transformation tests showed decreased reactivity only in patients with S-creatinine level greater than or equal to 230 micromol/l. The results suggest an association of chronic pyelonephritis with a defective efferent, nonspecific arm of cellular immunity.", "contents": "Cellular immunity in renal diseases. Cellular immune reactivity was studied in 78 patients with various forms of renal disease by skin testing with four recall antigens and a lymphocyte transformation test with tuberculin PPD and leucoagglutinin. Patients with S-creatine greater than or equal to 230 micromol/l as well as those with chronic pyelonephritis who had S-creatinine values below 230 micromol/l had significantly lower skin reactions than the controls to streptokinase-streptodornase, parotitis and PPD. Glomerulonephritic patients with S-creatinine values below 230 micromol/l had normal skin reactivity. Lymphocyte transformation tests showed decreased reactivity only in patients with S-creatinine level greater than or equal to 230 micromol/l. The results suggest an association of chronic pyelonephritis with a defective efferent, nonspecific arm of cellular immunity."}
{"id": "PMID:474187", "title": "Renal function and plasma aldosterone during acute lithium intoxication.", "content": "Studies on plasma aldosterone, total serum protein, electrolytes, osmolal concentrations in serum and urine, creatinine, lithium and osmolal clearances were carried out on 14 patients suffering from lithium intoxication. The determinations were done on samples obtained on admission and during the following twelve days of treatment with hemodialysis, sodium chloride loading, or forced diuresis. Plasma aldosterone and total serum protein were increased; serum sodium concentrations and creatinine clearances were decreased on admission. Serum osmolality was normal; urine osmolal concentration was just above that in plasma. The results showed that the lithium intoxicated patients were moderately depleted of sodium and water on admission. Plasma aldosterone, total serum protein and serum sodium were normalized during treatment. Creatinine clearance increased but did not normalize in all patients. Renal concentrating tests were performed in five patients three to four weeks after lithium intoxication. The renal concentrating ability was reduced in all. The elevated plasma aldosterone seen in lithium intoxication might have been a result of sodium and water depletion. It is suggested that impaired renal concentrating ability induced by lithium may make some patients more susceptible to conditions which can lead to sodium and water loss, and thereby to lithium intoxication.", "contents": "Renal function and plasma aldosterone during acute lithium intoxication. Studies on plasma aldosterone, total serum protein, electrolytes, osmolal concentrations in serum and urine, creatinine, lithium and osmolal clearances were carried out on 14 patients suffering from lithium intoxication. The determinations were done on samples obtained on admission and during the following twelve days of treatment with hemodialysis, sodium chloride loading, or forced diuresis. Plasma aldosterone and total serum protein were increased; serum sodium concentrations and creatinine clearances were decreased on admission. Serum osmolality was normal; urine osmolal concentration was just above that in plasma. The results showed that the lithium intoxicated patients were moderately depleted of sodium and water on admission. Plasma aldosterone, total serum protein and serum sodium were normalized during treatment. Creatinine clearance increased but did not normalize in all patients. Renal concentrating tests were performed in five patients three to four weeks after lithium intoxication. The renal concentrating ability was reduced in all. The elevated plasma aldosterone seen in lithium intoxication might have been a result of sodium and water depletion. It is suggested that impaired renal concentrating ability induced by lithium may make some patients more susceptible to conditions which can lead to sodium and water loss, and thereby to lithium intoxication."}
{"id": "PMID:474188", "title": "Mortality from chronic interstitial nephritis and phenacetin consumption in Denmark.", "content": "After 1950, a pronounced increase in the mortality from chronic interstitial nephritis among women was registered in Denmark. This was suspected to be caused by a contemporary increase in the consumption of drugs containing phenacetin. Restrictions were therefore imposed on the sale of such drugs in 1961 and consumption decreased sharply. This report, based upon death certificates from 1941--76, shows that the mortality rate from chronic interstitial nephritis has decreased gradually among women in Denmark since 1960, and has now reached the same level in younger women as in the 1940's.", "contents": "Mortality from chronic interstitial nephritis and phenacetin consumption in Denmark. After 1950, a pronounced increase in the mortality from chronic interstitial nephritis among women was registered in Denmark. This was suspected to be caused by a contemporary increase in the consumption of drugs containing phenacetin. Restrictions were therefore imposed on the sale of such drugs in 1961 and consumption decreased sharply. This report, based upon death certificates from 1941--76, shows that the mortality rate from chronic interstitial nephritis has decreased gradually among women in Denmark since 1960, and has now reached the same level in younger women as in the 1940's."}
{"id": "PMID:474189", "title": "1 alpha-hydroxyvitamin D3 treatment of therapy-resistant symptomatic hypocalcemia in a hypoparathyroid patient with intestinal malabsorption.", "content": "The case history of a hypoparathyroid female with short bowel syndrome and long-standing therapy-resistant symptomatic hypocalcemia is reported. During treatment with massive doses of the potent vitamin D analog, 1 alpha-hydroxyvitamin D3(1 alpha(OH)D3), normocalcemia was re-established and clinical symptoms of hypocalcemia were relieved. Furthermore, significant improvement of t of intestinal calcium absorption and bone mineral content was observed after three months of treatment with 1 alpha(OH)D3. The data suggest that 1 alpha(OH)D3 may be of therapeutical value in patients with hypoparathyroidism and intestinal malabsorption.", "contents": "1 alpha-hydroxyvitamin D3 treatment of therapy-resistant symptomatic hypocalcemia in a hypoparathyroid patient with intestinal malabsorption. The case history of a hypoparathyroid female with short bowel syndrome and long-standing therapy-resistant symptomatic hypocalcemia is reported. During treatment with massive doses of the potent vitamin D analog, 1 alpha-hydroxyvitamin D3(1 alpha(OH)D3), normocalcemia was re-established and clinical symptoms of hypocalcemia were relieved. Furthermore, significant improvement of t of intestinal calcium absorption and bone mineral content was observed after three months of treatment with 1 alpha(OH)D3. The data suggest that 1 alpha(OH)D3 may be of therapeutical value in patients with hypoparathyroidism and intestinal malabsorption."}
{"id": "PMID:474191", "title": "Serum calcitonin in patients with chronic renal disease.", "content": "The relationship between serum calcitonin, calcium-phosphorus metabolism and renal function was studied in 66 patients with chronic renal disease. Serum calcitonin was significantly elevated both in non-dialysed patients and in patients on chronic hemodialysis. In the non-dialysed patient group a highly significant inverse correlation was found between serum calcitonin and creatinine clearance. Between serum concentrations of calcitonin and phosphorus a significant positive correlation was found. In the dialysed patients a significant inverse correlation was found between serum calcium and serum calcitonin. It is concluded that the elevated serum calcitonin in patients with chronic renal disease might be explained by a reduced renal degradation of calcitonin and/or an increased production due to stimulation by serum phosphorus.", "contents": "Serum calcitonin in patients with chronic renal disease. The relationship between serum calcitonin, calcium-phosphorus metabolism and renal function was studied in 66 patients with chronic renal disease. Serum calcitonin was significantly elevated both in non-dialysed patients and in patients on chronic hemodialysis. In the non-dialysed patient group a highly significant inverse correlation was found between serum calcitonin and creatinine clearance. Between serum concentrations of calcitonin and phosphorus a significant positive correlation was found. In the dialysed patients a significant inverse correlation was found between serum calcium and serum calcitonin. It is concluded that the elevated serum calcitonin in patients with chronic renal disease might be explained by a reduced renal degradation of calcitonin and/or an increased production due to stimulation by serum phosphorus."}
{"id": "PMID:474192", "title": "Serum levels of testosterone and luteinizing hormone in patients with chronic renal disease.", "content": "Androgen metabolism has been studied in 33 patients (17 males, 16 females) with chronic renal disease not undergoing dialysis treatment. The mean value of serum testosterone was reduced in both sexes, whereas that of serum leteinizing hormone (LH) was elevated in the males. The parameters became increasingly pathological with decreasing renal function. There was no correlation between serum testosterone and serum LH, indicating an inadequate hypothalamic-pituitary response to the testicular dysfunction. The clinical significance of this relative hypoandrogenaemia is obscure. A possible relation to the anaemia and bone disease of chronic renal failure is discussed.", "contents": "Serum levels of testosterone and luteinizing hormone in patients with chronic renal disease. Androgen metabolism has been studied in 33 patients (17 males, 16 females) with chronic renal disease not undergoing dialysis treatment. The mean value of serum testosterone was reduced in both sexes, whereas that of serum leteinizing hormone (LH) was elevated in the males. The parameters became increasingly pathological with decreasing renal function. There was no correlation between serum testosterone and serum LH, indicating an inadequate hypothalamic-pituitary response to the testicular dysfunction. The clinical significance of this relative hypoandrogenaemia is obscure. A possible relation to the anaemia and bone disease of chronic renal failure is discussed."}
{"id": "PMID:474193", "title": "Iron absorption and iron status in patients with chronic uremia on regular peritoneal dialysis.", "content": "Gastrointestinal iron absorption was measured by whole body counting in 18 patients on regular peritoneal dialysis. Ten patients received regular oral iron treatment prior to the study (iron treated group), 8 patients did not receive iron treatment (non-iron treated group). Whole body retention 14 days after oral administration of 10 microCi 59Fe together with a carrier dose of 10 mg Fe2+ was used as an estimate of absorption. The erythrocyte iron incorporation, i.e. the percentage of administered 59Fe incorporated into the total erythrocyte mass, was measured. Geometric mean iron absorption in the non-iron treated groups was 7.4+/-3.3 (S.D.) % and in the iron treated group 2.8+/-2.5% (p less than 0.01). Absorption in the non-iron treated group did not differ significantly from the value in a normal control group (p greater than 0.3). Absorption in the iron treated group was distinctly lower than in the controls (p less than 0.01), due to the high iron supplementation. Several patients in the non-iron treated group had latent or overt iron deficiency, while patients in the iron treated group had satisfactory iron status. The correlation between iron absorption and erythrocyte iron incorporation was highly significant (r=0.95, p less than 0.001). Peritoneal dialysis patients on the whole have a normally functioning iron absorption. However, due to increased iron losses and insufficient dietary iron intake, the maintenance of a satisfactory iron balance implies an adequate oral iron supplementation.", "contents": "Iron absorption and iron status in patients with chronic uremia on regular peritoneal dialysis. Gastrointestinal iron absorption was measured by whole body counting in 18 patients on regular peritoneal dialysis. Ten patients received regular oral iron treatment prior to the study (iron treated group), 8 patients did not receive iron treatment (non-iron treated group). Whole body retention 14 days after oral administration of 10 microCi 59Fe together with a carrier dose of 10 mg Fe2+ was used as an estimate of absorption. The erythrocyte iron incorporation, i.e. the percentage of administered 59Fe incorporated into the total erythrocyte mass, was measured. Geometric mean iron absorption in the non-iron treated groups was 7.4+/-3.3 (S.D.) % and in the iron treated group 2.8+/-2.5% (p less than 0.01). Absorption in the non-iron treated group did not differ significantly from the value in a normal control group (p greater than 0.3). Absorption in the iron treated group was distinctly lower than in the controls (p less than 0.01), due to the high iron supplementation. Several patients in the non-iron treated group had latent or overt iron deficiency, while patients in the iron treated group had satisfactory iron status. The correlation between iron absorption and erythrocyte iron incorporation was highly significant (r=0.95, p less than 0.001). Peritoneal dialysis patients on the whole have a normally functioning iron absorption. However, due to increased iron losses and insufficient dietary iron intake, the maintenance of a satisfactory iron balance implies an adequate oral iron supplementation."}
{"id": "PMID:474194", "title": "Increased red cell content of Zn2+ in essential hypertension.", "content": "The zinc content in plasma as well as in erythrocytes was studied in 61 patients with untreated essential hypertension. They displayed an intraerythrocytic mean value of Zn2+ of 128.2 micromoles/l intracellular water, which is significantly higher than the corresponding mean of 101.5 micromoles/l from 42 normal persons. In plasma, on the other hand, the mean zinc value of the hypertensive persons was 15.2 micromoles/l plasma, i.e. well within the normal range of plasma Zn2+. Possible mechanisms underlying the elevated red cell zinc level in hypertension are discussed.", "contents": "Increased red cell content of Zn2+ in essential hypertension. The zinc content in plasma as well as in erythrocytes was studied in 61 patients with untreated essential hypertension. They displayed an intraerythrocytic mean value of Zn2+ of 128.2 micromoles/l intracellular water, which is significantly higher than the corresponding mean of 101.5 micromoles/l from 42 normal persons. In plasma, on the other hand, the mean zinc value of the hypertensive persons was 15.2 micromoles/l plasma, i.e. well within the normal range of plasma Zn2+. Possible mechanisms underlying the elevated red cell zinc level in hypertension are discussed."}
{"id": "PMID:474195", "title": "Resolution of renal amyloidosis secondary to rheumatoid arthritis.", "content": "A patient with seronegative rheumatoid arthritis developed a nephrotic syndrome. Histological examination of renal biopsy disclosed moderate amyloidosis. Ultrastructurally the glomerular amyloid deposits were seen to be located both within the mesangium and subepithelially in the peripheral capillaries. The patient was treated with prednisone and cyclophosphamide for two years. The nephrotic syndrome remitted and a follow-up biopsy showed almost total disappearance of Congo red positive amyloid substance. Electron microscopy showed abundant finely granular material but only small amounts of fibrillar amyloid in the mesangial regions and intramembranous lucent areas containing few amyloid fibrils but no subepithelial deposits in the peripheral capillaries. We conclude that the mesangial amyloid substance was degraded to granular material and that the subepithelial amyloid deposits were resolved by mechanisms similar to those involved in the resolution of subepithelial immune complex deposits, i.e. through slow washing out and incorporation into the basement membrane.", "contents": "Resolution of renal amyloidosis secondary to rheumatoid arthritis. A patient with seronegative rheumatoid arthritis developed a nephrotic syndrome. Histological examination of renal biopsy disclosed moderate amyloidosis. Ultrastructurally the glomerular amyloid deposits were seen to be located both within the mesangium and subepithelially in the peripheral capillaries. The patient was treated with prednisone and cyclophosphamide for two years. The nephrotic syndrome remitted and a follow-up biopsy showed almost total disappearance of Congo red positive amyloid substance. Electron microscopy showed abundant finely granular material but only small amounts of fibrillar amyloid in the mesangial regions and intramembranous lucent areas containing few amyloid fibrils but no subepithelial deposits in the peripheral capillaries. We conclude that the mesangial amyloid substance was degraded to granular material and that the subepithelial amyloid deposits were resolved by mechanisms similar to those involved in the resolution of subepithelial immune complex deposits, i.e. through slow washing out and incorporation into the basement membrane."}
{"id": "PMID:474197", "title": "Anthropometric data in middle-aged women. The population study of women in G\u00f6teborg 1968-1969.", "content": "Body weight, body height, skinfolds, circumferences and weight indices in a population sample of 1462 women in ages between 38 and 60 years are presented. Significant age differences were noted. High correlations (r) were found between body weight and triceps and subscapular skinfolds (0.62 and 0.72 respectively) and between body weight and arm, waist and buttock circumferences (0.75-0.88). Age differences were mostly noted between 46 and 50 years of age. After that age there was a comparatively greater increase of the waist circumference than of the buttock circumference, while triceps skinfold seemed to decrease after the age of 50 indicating an altered adipose tissue distribution from the extremities to the trunk with increasing age. When taking data from a previous study of body weight in the same population of women into consideration it seems that the age differences in body weight found in cross-sectional study like the present one may to a large extent depend on differences between different cohorts studied.", "contents": "Anthropometric data in middle-aged women. The population study of women in G\u00f6teborg 1968-1969. Body weight, body height, skinfolds, circumferences and weight indices in a population sample of 1462 women in ages between 38 and 60 years are presented. Significant age differences were noted. High correlations (r) were found between body weight and triceps and subscapular skinfolds (0.62 and 0.72 respectively) and between body weight and arm, waist and buttock circumferences (0.75-0.88). Age differences were mostly noted between 46 and 50 years of age. After that age there was a comparatively greater increase of the waist circumference than of the buttock circumference, while triceps skinfold seemed to decrease after the age of 50 indicating an altered adipose tissue distribution from the extremities to the trunk with increasing age. When taking data from a previous study of body weight in the same population of women into consideration it seems that the age differences in body weight found in cross-sectional study like the present one may to a large extent depend on differences between different cohorts studied."}
{"id": "PMID:474199", "title": "A scanning electron microscopy study of vascular casts from senile human brains.", "content": "A scanning electron microscope study was performed of vascular casts from 26 senile human brains. In 15 of these, three types of arterial deformities (glomerular loop formations, vascular wickerworks and bundles) were frequently encountered. They were compared with the appearance in microangiograms and histological sections.", "contents": "A scanning electron microscopy study of vascular casts from senile human brains. A scanning electron microscope study was performed of vascular casts from 26 senile human brains. In 15 of these, three types of arterial deformities (glomerular loop formations, vascular wickerworks and bundles) were frequently encountered. They were compared with the appearance in microangiograms and histological sections."}
{"id": "PMID:474201", "title": "Classification of vertebro-basilar aneurysms.", "content": "In continuation of earlier studies concerning the aneurysms of ICA and PCA a proposed detailed classification of vertebro-basilar aneurysms has been given. It has been proved that nearly all the aneurysms originate at the divisions of the main arteries or at the ends of large arteries as direct continuations of the main branches (BA as well as ICA). Aneurysms of the vertebro-basilar junction are of special aetiological interest. These last aneurysms are unique, and demonstrate that congenital defects seem to be more important for the origin of aneurysms than the direction of the blood flow. Origin and projection characterize the different types. Atypically located aneurysms away from arterial divisions exist, as in the ICA system. The relations between aneurysms and the multiplicity of variations and anomalies of the main arteries and the posterior part of the circle of Willis are still unknown, and should be checked in detail. Our studies have convinced us that the exact knowledge of microtopographic classification is the key to correct preoperative diagnosis, access, and treatment.", "contents": "Classification of vertebro-basilar aneurysms. In continuation of earlier studies concerning the aneurysms of ICA and PCA a proposed detailed classification of vertebro-basilar aneurysms has been given. It has been proved that nearly all the aneurysms originate at the divisions of the main arteries or at the ends of large arteries as direct continuations of the main branches (BA as well as ICA). Aneurysms of the vertebro-basilar junction are of special aetiological interest. These last aneurysms are unique, and demonstrate that congenital defects seem to be more important for the origin of aneurysms than the direction of the blood flow. Origin and projection characterize the different types. Atypically located aneurysms away from arterial divisions exist, as in the ICA system. The relations between aneurysms and the multiplicity of variations and anomalies of the main arteries and the posterior part of the circle of Willis are still unknown, and should be checked in detail. Our studies have convinced us that the exact knowledge of microtopographic classification is the key to correct preoperative diagnosis, access, and treatment."}
{"id": "PMID:474202", "title": "Aneurysm surgery above the eighth decade.", "content": "A series of nine patients over 70 years of age with saccular aneurysms, operated on by direct surgery, is reviewed in order to discuss aneurysm surgery in older patients. The median age of the patients was 74 years, and the median interval from haemorrhage to surgery was 18 days. Operative mortality was 11%, and morbidity was 33%. Because of arteriosclerosis round the aneurysm neck and parent artery, special considerations for surgical treatment on the older patients are required.", "contents": "Aneurysm surgery above the eighth decade. A series of nine patients over 70 years of age with saccular aneurysms, operated on by direct surgery, is reviewed in order to discuss aneurysm surgery in older patients. The median age of the patients was 74 years, and the median interval from haemorrhage to surgery was 18 days. Operative mortality was 11%, and morbidity was 33%. Because of arteriosclerosis round the aneurysm neck and parent artery, special considerations for surgical treatment on the older patients are required."}
{"id": "PMID:474204", "title": "Value of electroencephalography (EEG) before and after surgery of intracranial aneurysms.", "content": "Electroencephalographic (EEG) investigations were done in 36 patients with intracranial aneurysms, before and after surgery. Thirty five of them suffered from subarachnoid hemorrhage (SAH). Pre-operatively, there was no correlation between patients' age or sex and localisation of the aneurysm or degree of EEG disturbances. The most frequent finding was a generalised slowing, the degree of which depended on the time from bleeding to EEG. Focal abnormalities were due to spasms of the vessels or intracerebral haematomas. There was a high correlation with neurological deficits. Post-operatively, EEG disturbances became worse in 21 cases. Generalised and focal abnormalities increased. These were due to focal oedema and the operative approach. The EEG could be correlated very well with the findings from other investigative methods (CT scanning, angiography). The EEG, as a functional method, showed very well the whole state of the brain after bleeding and after operation.", "contents": "Value of electroencephalography (EEG) before and after surgery of intracranial aneurysms. Electroencephalographic (EEG) investigations were done in 36 patients with intracranial aneurysms, before and after surgery. Thirty five of them suffered from subarachnoid hemorrhage (SAH). Pre-operatively, there was no correlation between patients' age or sex and localisation of the aneurysm or degree of EEG disturbances. The most frequent finding was a generalised slowing, the degree of which depended on the time from bleeding to EEG. Focal abnormalities were due to spasms of the vessels or intracerebral haematomas. There was a high correlation with neurological deficits. Post-operatively, EEG disturbances became worse in 21 cases. Generalised and focal abnormalities increased. These were due to focal oedema and the operative approach. The EEG could be correlated very well with the findings from other investigative methods (CT scanning, angiography). The EEG, as a functional method, showed very well the whole state of the brain after bleeding and after operation."}
{"id": "PMID:474205", "title": "Persistent embryonic veins in the arteriovenous malformation of the diencephalon.", "content": "The diencephalic arteriovenous malformation is produced by the posterior cerebral, posterior choroidal, and anterior choroidal arteries. The abnormal arterialised vessels seen in these malformation represent dorsal and ventral diencephalic veins. The drainage of the shunted blood is primarily into the transverse sinus. This is later diverted medially into the great cerebral vein, with the formation of the basal vein. The medial drainage, in keeping with the normal developmental pattern, is seen frequently. The presence and locations of angiomas are very variable and do not follow any predictable pattern.", "contents": "Persistent embryonic veins in the arteriovenous malformation of the diencephalon. The diencephalic arteriovenous malformation is produced by the posterior cerebral, posterior choroidal, and anterior choroidal arteries. The abnormal arterialised vessels seen in these malformation represent dorsal and ventral diencephalic veins. The drainage of the shunted blood is primarily into the transverse sinus. This is later diverted medially into the great cerebral vein, with the formation of the basal vein. The medial drainage, in keeping with the normal developmental pattern, is seen frequently. The presence and locations of angiomas are very variable and do not follow any predictable pattern."}
{"id": "PMID:474207", "title": "Acquired hydrocephalus. III. A pathophysiological study correlated with neuropathological findings and clinical manifestations.", "content": "On the basis of the laws of Pascal and Laplace, it is shown that the ventricular dilatation in acquired hydrocephalus is due to a primary increase in the intraventricular pressure (IVP), and that a new steady state can be reached, whether the IVP is increased or normal. The pressure increase is due to a disproportion between the production and reabsorption of cerebrospinal fluid (CSF). As water and salts pass freely across the ependyma and the choroid plexus in hydrocephalus, the pressure increase is caused by an increased protein concentration in the ventricular CSF, leading to increased fluid contents according to the Gibbs-Donnan equilibrium. During the ventricular dilatation, the ependyma is destroyed, and the protein molecules penetrate into the subependymal part of the white matter. This results in a reduction in the colloid osmotic pressure of the ventricular CSF, and a new steady state can be reached, with a normal protein concentration in an increased volume. The attendant microscopic changes in the ventricular wall were demonstrated in a patient with acquired hydrocephalus, and the observations made were in conformity with the results of a number of animal experiments. The symptomatology of acquired hydrocephalus is in agreement with a primary affection of the axons running in the juxtaventricular part of the white matter.", "contents": "Acquired hydrocephalus. III. A pathophysiological study correlated with neuropathological findings and clinical manifestations. On the basis of the laws of Pascal and Laplace, it is shown that the ventricular dilatation in acquired hydrocephalus is due to a primary increase in the intraventricular pressure (IVP), and that a new steady state can be reached, whether the IVP is increased or normal. The pressure increase is due to a disproportion between the production and reabsorption of cerebrospinal fluid (CSF). As water and salts pass freely across the ependyma and the choroid plexus in hydrocephalus, the pressure increase is caused by an increased protein concentration in the ventricular CSF, leading to increased fluid contents according to the Gibbs-Donnan equilibrium. During the ventricular dilatation, the ependyma is destroyed, and the protein molecules penetrate into the subependymal part of the white matter. This results in a reduction in the colloid osmotic pressure of the ventricular CSF, and a new steady state can be reached, with a normal protein concentration in an increased volume. The attendant microscopic changes in the ventricular wall were demonstrated in a patient with acquired hydrocephalus, and the observations made were in conformity with the results of a number of animal experiments. The symptomatology of acquired hydrocephalus is in agreement with a primary affection of the axons running in the juxtaventricular part of the white matter."}
{"id": "PMID:474208", "title": "A new surgical approach to the third ventricle with interruption of the striothalamic vein.", "content": "In the removal of tumours that develop within the third ventricle, most approaches are not entirely satisfactory. Therefore, a new approach has been devised: transfrontal exposure of the anterior portion of the frontal horn; coagulation and section of the striothalamic vein in order to open up the roof of the third ventricle; use of a blunt spatula introduced in the foramen of Monro and pushed backwards under the choro\u00efd plexus. This approach has been used in ten cases. Postoperative mortality has been nil; the surgical approach has not apparently been responsible for any sequelae. It is simple and gives a good view of the third ventricle.", "contents": "A new surgical approach to the third ventricle with interruption of the striothalamic vein. In the removal of tumours that develop within the third ventricle, most approaches are not entirely satisfactory. Therefore, a new approach has been devised: transfrontal exposure of the anterior portion of the frontal horn; coagulation and section of the striothalamic vein in order to open up the roof of the third ventricle; use of a blunt spatula introduced in the foramen of Monro and pushed backwards under the choro\u00efd plexus. This approach has been used in ten cases. Postoperative mortality has been nil; the surgical approach has not apparently been responsible for any sequelae. It is simple and gives a good view of the third ventricle."}
{"id": "PMID:474209", "title": "Unilateral inferior temporal lobectomy with hippocampectomy for relief of incisural herniation.", "content": "1. The syndrome of incisural hippocampal herniation, accompanied by unilateral and then bilateral fixed pupils, deepening coma, respiratory irregularity (Cheyne-Stokes), contralateral hemiplegia, ipsilateral decerebrate posturing rising blood pressure, and finally renal shutdown, indicates a terminal state that requires immediate relief. 2. Accepted methods of relief by brain shrinkage, dexamethasone, subtemporal decompression, anterior temporal lobectomy, cutting of the tentorium, and even massive uncapping of the skull, have failed in the author's experience to reverse the process when once established. 3. Presentation is made of a rapid and relatively simple inferior horizontal temporal lobectomy, including the hippocampal gyrus, so as to relieve peduncle compression, blockage of cerebrospinal fluid circulation, midbrain haemorrhage, and infarction in the posterior cerebral artery distribution area. 4. Reports are given of 15 cases out of 30 cases in which operation proved lifesaving, with restoration of normal function in 13. 5. Those patients who died did so in an average of five days, and generally showed either no herniation or continuing evidence of tumour or internal bleeding. 6. Very young patients particularly have an excellent chance of survival, which is seven times better than that of adults in having good to excellent results without operation.", "contents": "Unilateral inferior temporal lobectomy with hippocampectomy for relief of incisural herniation. 1. The syndrome of incisural hippocampal herniation, accompanied by unilateral and then bilateral fixed pupils, deepening coma, respiratory irregularity (Cheyne-Stokes), contralateral hemiplegia, ipsilateral decerebrate posturing rising blood pressure, and finally renal shutdown, indicates a terminal state that requires immediate relief. 2. Accepted methods of relief by brain shrinkage, dexamethasone, subtemporal decompression, anterior temporal lobectomy, cutting of the tentorium, and even massive uncapping of the skull, have failed in the author's experience to reverse the process when once established. 3. Presentation is made of a rapid and relatively simple inferior horizontal temporal lobectomy, including the hippocampal gyrus, so as to relieve peduncle compression, blockage of cerebrospinal fluid circulation, midbrain haemorrhage, and infarction in the posterior cerebral artery distribution area. 4. Reports are given of 15 cases out of 30 cases in which operation proved lifesaving, with restoration of normal function in 13. 5. Those patients who died did so in an average of five days, and generally showed either no herniation or continuing evidence of tumour or internal bleeding. 6. Very young patients particularly have an excellent chance of survival, which is seven times better than that of adults in having good to excellent results without operation."}
{"id": "PMID:474210", "title": "The management of obstructive hydrocephalus by the use of external continuous ventricular drainage.", "content": "External continuous ventricular drainage (CVD) in patients suffering from obstructive hydrocephalus is being used in many neurosurgical centers as a routine procedure for the temporary relief of intracranial pressure. The results of this treatment have been insufficiently evaluated. In the present study two groups of patients were investigated. In one, CVD was never used. In the other one it was used in all cases. It was found that the use of CVD decreased mortality rate most significantly. It is recommended that CVD should be used as a temporary measure for the treatment of the increased intracranial pressure in patients suffering from obstructive hydrocephalus.", "contents": "The management of obstructive hydrocephalus by the use of external continuous ventricular drainage. External continuous ventricular drainage (CVD) in patients suffering from obstructive hydrocephalus is being used in many neurosurgical centers as a routine procedure for the temporary relief of intracranial pressure. The results of this treatment have been insufficiently evaluated. In the present study two groups of patients were investigated. In one, CVD was never used. In the other one it was used in all cases. It was found that the use of CVD decreased mortality rate most significantly. It is recommended that CVD should be used as a temporary measure for the treatment of the increased intracranial pressure in patients suffering from obstructive hydrocephalus."}
{"id": "PMID:474211", "title": "Significance of intravascular and intracardiac movement of the cardiac catheter in ventriculo-atrial shunts (review of 655 cases).", "content": "Proper placement of ventriculo-atrial shunts can be complicated by three phenomena: 1. Movement of the cardiac catheter intravascularly into the internal jugular vein as noted on chest X-ray. 2. Movement of the cardiac catheter during intraoperative X-ray leading to blurring and hence problems in accurate localization. 3. No sign on X-ray of the catheter tip or even of the catheter itself despite previous X-ray visualization and good function, a problem to be obviated by better radiopaque markings.", "contents": "Significance of intravascular and intracardiac movement of the cardiac catheter in ventriculo-atrial shunts (review of 655 cases). Proper placement of ventriculo-atrial shunts can be complicated by three phenomena: 1. Movement of the cardiac catheter intravascularly into the internal jugular vein as noted on chest X-ray. 2. Movement of the cardiac catheter during intraoperative X-ray leading to blurring and hence problems in accurate localization. 3. No sign on X-ray of the catheter tip or even of the catheter itself despite previous X-ray visualization and good function, a problem to be obviated by better radiopaque markings."}
{"id": "PMID:474213", "title": "Subarachnoid pouches of the posterior fossa with syringomyelia.", "content": "Three men are presented, all of whom were born with difficulty and later developed severe syringomyelia with collapsing cords on air myelography. Each had hydrocephalus and a sizable subarachnoid pouch in the posterior fossa. The clinical features and investigations are presented with a discussion of the aetiology and possible mechanisms concerned in pathogenesis and treatment. All three men had posterior fossa exploration over fifty years after birth, and the first two were also treated by valved ventriculo-atrial shunts. Each patient has improved a little since operation, but no certainty that this was due to surgery has been proved in any case; none has progressed since the last operation.", "contents": "Subarachnoid pouches of the posterior fossa with syringomyelia. Three men are presented, all of whom were born with difficulty and later developed severe syringomyelia with collapsing cords on air myelography. Each had hydrocephalus and a sizable subarachnoid pouch in the posterior fossa. The clinical features and investigations are presented with a discussion of the aetiology and possible mechanisms concerned in pathogenesis and treatment. All three men had posterior fossa exploration over fifty years after birth, and the first two were also treated by valved ventriculo-atrial shunts. Each patient has improved a little since operation, but no certainty that this was due to surgery has been proved in any case; none has progressed since the last operation."}
{"id": "PMID:474215", "title": "Regional cerebral blood flow in patients with subarachnoid haemorrhage.", "content": "With a multidetector scintillation camera regional cerebral blood flow, rCBF, was evaluated in 13 patients with subarachnoid haemorrhage. Mean CBF was subnormal, and there seems to be a relationship between CBF and severity of the neurological deficit. The regional pattern showed ischaemic as well as hyperaemic areas. Vasospasms mostly produced ischaemic areas, roughly corresponding to the region supplied by the involved artery. The hyperaemic areas were striking. This hyperaemia can perhaps be regarded as a reaction to initial ischaemia.", "contents": "Regional cerebral blood flow in patients with subarachnoid haemorrhage. With a multidetector scintillation camera regional cerebral blood flow, rCBF, was evaluated in 13 patients with subarachnoid haemorrhage. Mean CBF was subnormal, and there seems to be a relationship between CBF and severity of the neurological deficit. The regional pattern showed ischaemic as well as hyperaemic areas. Vasospasms mostly produced ischaemic areas, roughly corresponding to the region supplied by the involved artery. The hyperaemic areas were striking. This hyperaemia can perhaps be regarded as a reaction to initial ischaemia."}
{"id": "PMID:474216", "title": "Effect of reperfusion on the uptake of [3H] uridine in the gerbil brain after prolonged ischaemia.", "content": "The uptake of labelled uridine is reduced in the whole ischaemic hemispheres of gerbils subjected to unilateral carotid artery occlusion. Following circulatory restoration, brain structures that had an ischaemic insult of moderate intensity exhibit a progressively increased uptake. However, during reperfusion there is a tendency towards a clear cut definition of dead zones. This progression of the lesions seems to be related to a maturation phenomenon occurring in areas with an irreversible damage at the end of the ischaemic period.", "contents": "Effect of reperfusion on the uptake of [3H] uridine in the gerbil brain after prolonged ischaemia. The uptake of labelled uridine is reduced in the whole ischaemic hemispheres of gerbils subjected to unilateral carotid artery occlusion. Following circulatory restoration, brain structures that had an ischaemic insult of moderate intensity exhibit a progressively increased uptake. However, during reperfusion there is a tendency towards a clear cut definition of dead zones. This progression of the lesions seems to be related to a maturation phenomenon occurring in areas with an irreversible damage at the end of the ischaemic period."}
{"id": "PMID:474217", "title": "Persistent embryonic veins in the arteriovenous malformation of the telencephalon.", "content": "The arterial components of the telencephalic arteriovenous malformations arise from the anterior cerebral and middle cerebral arteries. The complexity of the development process in this region obscures the fundamental segmental pattern. There are three regions where the primitive pial vessels cross at right angles. They are: a) over the convexity of the cerebral hemispheres, b) in the Sylvian territory, c) in the region of the basal ganglia. The malformations over the surface drain into the superior sagittal sinus. The Sylvian and deep telencephalic malformations drain primarily into the tentorial sinus, and this is often replaced by a medial drainage into the internal cerebral vein through the tributaries of the basal vein.", "contents": "Persistent embryonic veins in the arteriovenous malformation of the telencephalon. The arterial components of the telencephalic arteriovenous malformations arise from the anterior cerebral and middle cerebral arteries. The complexity of the development process in this region obscures the fundamental segmental pattern. There are three regions where the primitive pial vessels cross at right angles. They are: a) over the convexity of the cerebral hemispheres, b) in the Sylvian territory, c) in the region of the basal ganglia. The malformations over the surface drain into the superior sagittal sinus. The Sylvian and deep telencephalic malformations drain primarily into the tentorial sinus, and this is often replaced by a medial drainage into the internal cerebral vein through the tributaries of the basal vein."}
{"id": "PMID:474218", "title": "Relevance of antibiotics in the treatment of brain abscesses. Report of a case with eight simultaneous brain abscesses treated and cured medically.", "content": "A case of eight simultaneous brain abscesses in the right cerebral hemisphere, treated medically after puncture of two of them, is reported. Antibiotics and hypertonic mannitol and, after seven days, dexamethasone were sufficient to cure these lesions. CT scan was of primary importance to follow the evolution under treatment. To get the best efficiency from antibiotics, identification of the microorganism and assessment of its resistance to antibiotics are necessary. It is suggested that in certain conditions medical therapy might be sufficient to treat cerebral abscess, after simple puncture to isolate the infecting agent. Corticosteroids should be avoided in the acute phase because they prevent antibiotics from penetrating the abscesses.", "contents": "Relevance of antibiotics in the treatment of brain abscesses. Report of a case with eight simultaneous brain abscesses treated and cured medically. A case of eight simultaneous brain abscesses in the right cerebral hemisphere, treated medically after puncture of two of them, is reported. Antibiotics and hypertonic mannitol and, after seven days, dexamethasone were sufficient to cure these lesions. CT scan was of primary importance to follow the evolution under treatment. To get the best efficiency from antibiotics, identification of the microorganism and assessment of its resistance to antibiotics are necessary. It is suggested that in certain conditions medical therapy might be sufficient to treat cerebral abscess, after simple puncture to isolate the infecting agent. Corticosteroids should be avoided in the acute phase because they prevent antibiotics from penetrating the abscesses."}
{"id": "PMID:474273", "title": "The immunoendocrinology of estrophilin.", "content": "Immunoglobulin from the serum of rabbits immunized with highly purified estradiol-receptor complex from calf uterine nuclei has been shown to contain specific antibodies to estrophilin by five criteria. Antibodies to calf nuclear estrophilin cross react with nuclear estradiol-receptor complexes of rat, rabbit and sheep uterus, rat endometrial and pituitary tumor, and MCF-7 human breast cancer cell line. They also react with extranuclear receptor of calf, rat, mouse, rabbit, guinea pig, monkey and sheep uterus, rat mammary, endometrial and pituitary tumor, and human breast cancer. There is no interaction of the antibody with estradiol itself. The nuclear form of estrophilin appears to bind more immunoglobulin molecules than does the cytosol form. The antibodies do not react with either the nuclear or extranuclear dihydrotestosterone-receptor complexes of rat prostate, with the extranuclear progesterone-receptor complexes of rabbit uterus, chick oviduct or rat endometrial tumor, or with rat and mouse alpha-fetoprotein. These findings indicate an immunochemical similarity among estrophilins from several mammalian species, as well as between nuclear and extranuclear forms of the receptor, but not among receptor proteins for different steroid hormones. Immunoglobulin from the serum of a goat immunized with similar antigen shows a considerably higher titer of antibodies to estrophilin. These react with nuclear and extranuclear estradiol-receptor complexes of calf uterus to produce somewhat larger entities than those formed with the rabbit antibody. Unlike the rabbit antibody, interaction with the goat antibody causes a noticeable decrease in estradiol-binding affinity of the extranuclear estrophilin as well as an apparent decrease in the total hormone-binding capacity. Specific antibodies to estrophilin offer promise as valuable reagents for receptor analysis and purification, as well as for the elucidation of many still unresolved questions concerning receptor synthesis, localization and function.", "contents": "The immunoendocrinology of estrophilin. Immunoglobulin from the serum of rabbits immunized with highly purified estradiol-receptor complex from calf uterine nuclei has been shown to contain specific antibodies to estrophilin by five criteria. Antibodies to calf nuclear estrophilin cross react with nuclear estradiol-receptor complexes of rat, rabbit and sheep uterus, rat endometrial and pituitary tumor, and MCF-7 human breast cancer cell line. They also react with extranuclear receptor of calf, rat, mouse, rabbit, guinea pig, monkey and sheep uterus, rat mammary, endometrial and pituitary tumor, and human breast cancer. There is no interaction of the antibody with estradiol itself. The nuclear form of estrophilin appears to bind more immunoglobulin molecules than does the cytosol form. The antibodies do not react with either the nuclear or extranuclear dihydrotestosterone-receptor complexes of rat prostate, with the extranuclear progesterone-receptor complexes of rabbit uterus, chick oviduct or rat endometrial tumor, or with rat and mouse alpha-fetoprotein. These findings indicate an immunochemical similarity among estrophilins from several mammalian species, as well as between nuclear and extranuclear forms of the receptor, but not among receptor proteins for different steroid hormones. Immunoglobulin from the serum of a goat immunized with similar antigen shows a considerably higher titer of antibodies to estrophilin. These react with nuclear and extranuclear estradiol-receptor complexes of calf uterus to produce somewhat larger entities than those formed with the rabbit antibody. Unlike the rabbit antibody, interaction with the goat antibody causes a noticeable decrease in estradiol-binding affinity of the extranuclear estrophilin as well as an apparent decrease in the total hormone-binding capacity. Specific antibodies to estrophilin offer promise as valuable reagents for receptor analysis and purification, as well as for the elucidation of many still unresolved questions concerning receptor synthesis, localization and function."}
{"id": "PMID:474274", "title": "Estrogen and antiestrogen action: studies in reproductive target tissues and tumors.", "content": "Studies are reported using unlabelled and radiolabelled antiestrogens which are aimed at elucidating the nature of interaction of antiestrogens with uterine and mammary tissue. Antiestrogens and long-acting estrogens move estrogen receptor sites to the nucleus and maintain elevated nuclear receptor levels for a prolonged period. The antagonistic action of antiestrogens in the uterus, however, appears to derive from their ability to effect a marked perturbation in the subcellular distribution of receptor whereby very little (ca. 10%) is cytoplasmic and further estrogen receptor accumulation is blocked. In DMBA-induced rat mammary tumors, the nonphototoxic antiestrogen, U-23, 469, effectively antagonizes the development and growth of tumors and elicits regression of 90% of established tumors with a time course similar to that seen after ovariectomy. Again, during antiestrogen treatment, the levels of cytoplasmic estrogen receptor are depressed with over 90% of total receptor being found in the nucleus. Two radiolabelled antiestrogens of high specific activity and purity have been prepared by us and their interaction with nuclear and cytoplasmic estrogen receptors is reported. Both 3H-CI-628 and 3H-U-23, 469 are metabolized in vivo to more polar forms which are found associated with the nuclear estrogen receptor, and which may be the true agents active in vivo.", "contents": "Estrogen and antiestrogen action: studies in reproductive target tissues and tumors. Studies are reported using unlabelled and radiolabelled antiestrogens which are aimed at elucidating the nature of interaction of antiestrogens with uterine and mammary tissue. Antiestrogens and long-acting estrogens move estrogen receptor sites to the nucleus and maintain elevated nuclear receptor levels for a prolonged period. The antagonistic action of antiestrogens in the uterus, however, appears to derive from their ability to effect a marked perturbation in the subcellular distribution of receptor whereby very little (ca. 10%) is cytoplasmic and further estrogen receptor accumulation is blocked. In DMBA-induced rat mammary tumors, the nonphototoxic antiestrogen, U-23, 469, effectively antagonizes the development and growth of tumors and elicits regression of 90% of established tumors with a time course similar to that seen after ovariectomy. Again, during antiestrogen treatment, the levels of cytoplasmic estrogen receptor are depressed with over 90% of total receptor being found in the nucleus. Two radiolabelled antiestrogens of high specific activity and purity have been prepared by us and their interaction with nuclear and cytoplasmic estrogen receptors is reported. Both 3H-CI-628 and 3H-U-23, 469 are metabolized in vivo to more polar forms which are found associated with the nuclear estrogen receptor, and which may be the true agents active in vivo."}
{"id": "PMID:474275", "title": "Inhibition of cell division and stimulation of progesterone receptor synthesis in rat oestrogen target tissues by non-steroidal antioestrogens.", "content": "Recent studies from this laboratory have demonstrated oestradiol-oestrogen receptors associated with the nuclear compartment of the rat uterine cell will initiate protein synthesis, as evidenced by a rise in progesterone receptor concentrations, and cell division whereas the anti-oestrogen - oestrogen receptor complex causes protein synthesis and cellular hypertrophy rather than hyperplasia. It is probable that this separation of biological activities resides in the intrinsic activity of the respective receptor complexes. We have demonstrated that caution should be exercised in the interpretation of low affinity ligand-hormone receptor interactions undertaken in vitro. Simple tests for ligand specificity for a binding protein are clearly insufficient evidence to characterise a hormone receptor complex using a conventional 15 hr technique of sucrose density gradient analysis. Oestrogens and anti-oestrogens do not seem to disrupt the subunit integrity of the cytoplasmic oestrogen receptor and it appears likely that the ligand plays a fundamental role in confering the correct biological properties to the hormone receptor complex.", "contents": "Inhibition of cell division and stimulation of progesterone receptor synthesis in rat oestrogen target tissues by non-steroidal antioestrogens. Recent studies from this laboratory have demonstrated oestradiol-oestrogen receptors associated with the nuclear compartment of the rat uterine cell will initiate protein synthesis, as evidenced by a rise in progesterone receptor concentrations, and cell division whereas the anti-oestrogen - oestrogen receptor complex causes protein synthesis and cellular hypertrophy rather than hyperplasia. It is probable that this separation of biological activities resides in the intrinsic activity of the respective receptor complexes. We have demonstrated that caution should be exercised in the interpretation of low affinity ligand-hormone receptor interactions undertaken in vitro. Simple tests for ligand specificity for a binding protein are clearly insufficient evidence to characterise a hormone receptor complex using a conventional 15 hr technique of sucrose density gradient analysis. Oestrogens and anti-oestrogens do not seem to disrupt the subunit integrity of the cytoplasmic oestrogen receptor and it appears likely that the ligand plays a fundamental role in confering the correct biological properties to the hormone receptor complex."}
{"id": "PMID:474276", "title": "Nuclear binding of the estrogen receptor: heterogeneity of sites and uterotropic response.", "content": "Two kinds of estradiol binding sites are present in purified nuclei from the rat uterus following estradiol injection. One of these sites (type I) corresponds to the well-known estrogen receptor which undergoes translocation from the cytoplasm to the nucleus. The second site (type II) is not translocated from the cytoplasm to the nucleus, however, estradiol treatment does stimulate an increased number of these sites. Type II sites are observed in purified nuclei and chromatin isolated from the uterus but not from non-target tissues such as the spleen and diaphragm. Thus an elevation in the levels of type II sites appear to be a specific nuclear response of the rat uterus to estradiol. Saturation analysis over a wide range of [3H]-estradiol concentrations produces a binding curve for type II sites which is sigmoidal and hence no accurate estimation of the dissociation constant is possible. The binding of [3H]-estradiol to nuclear type II sites is inhibited by estradiol and diethylstilbestrol but not by progesterone, testosterone, or corticosterone. Extraction of nuclei isolated from estrogen treated rat uteri with KCl provides a complex picture. Direct labeling of nuclear estrogen receptors either by in vivo injection or in vitro incubation of intact uteri with [3H]-estradiol measures only a fraction of the specific estrogen binding sites associated with the nuclear pellet following 0.4 M KCl extraction. These sites are more accurately determined by performing saturation analysis over a wide range of [3H]-estradiol concentrations by exchange which measures specific estrogen binding sites, not [3H]-steroid. Saturation analysis of estradiol binding to KCl extracted nuclei when performed by exchange, with appropriate corrections for type II binding, reveals that approximately 1000--2000 receptors per nucleus are resistant to KCl extraction 1 hr after administration. The same numbers of type I sites display long-term nuclear retention. A single injection of estradiol results in long term (greater than 6 h) retention of type I sites, rapid and sustained elevations (1--72h) in type II sites and true uterine growth (uterine wet weight at 24--43 h). Estriol injections caused a rapid increase in nuclear type I sites which was not accompanied by an increase in type II sites and no true uterine growth occurred. Administration of estriol or estradiol as a pellet implant, which causes continuous occupancy of type I sites, increases the quantity of nuclear type II sites and stimulates true uterine growth. Therefore, we conclude that elevated levels of nuclear type II sites correlate with the long term uterotropic response to estrogenic hormones. Although we do not understand the function of this second class of binding sites it is possible that the type II sites represent a major component in the mechanism by which estrogens stimulate growth of the uterus.", "contents": "Nuclear binding of the estrogen receptor: heterogeneity of sites and uterotropic response. Two kinds of estradiol binding sites are present in purified nuclei from the rat uterus following estradiol injection. One of these sites (type I) corresponds to the well-known estrogen receptor which undergoes translocation from the cytoplasm to the nucleus. The second site (type II) is not translocated from the cytoplasm to the nucleus, however, estradiol treatment does stimulate an increased number of these sites. Type II sites are observed in purified nuclei and chromatin isolated from the uterus but not from non-target tissues such as the spleen and diaphragm. Thus an elevation in the levels of type II sites appear to be a specific nuclear response of the rat uterus to estradiol. Saturation analysis over a wide range of [3H]-estradiol concentrations produces a binding curve for type II sites which is sigmoidal and hence no accurate estimation of the dissociation constant is possible. The binding of [3H]-estradiol to nuclear type II sites is inhibited by estradiol and diethylstilbestrol but not by progesterone, testosterone, or corticosterone. Extraction of nuclei isolated from estrogen treated rat uteri with KCl provides a complex picture. Direct labeling of nuclear estrogen receptors either by in vivo injection or in vitro incubation of intact uteri with [3H]-estradiol measures only a fraction of the specific estrogen binding sites associated with the nuclear pellet following 0.4 M KCl extraction. These sites are more accurately determined by performing saturation analysis over a wide range of [3H]-estradiol concentrations by exchange which measures specific estrogen binding sites, not [3H]-steroid. Saturation analysis of estradiol binding to KCl extracted nuclei when performed by exchange, with appropriate corrections for type II binding, reveals that approximately 1000--2000 receptors per nucleus are resistant to KCl extraction 1 hr after administration. The same numbers of type I sites display long-term nuclear retention. A single injection of estradiol results in long term (greater than 6 h) retention of type I sites, rapid and sustained elevations (1--72h) in type II sites and true uterine growth (uterine wet weight at 24--43 h). Estriol injections caused a rapid increase in nuclear type I sites which was not accompanied by an increase in type II sites and no true uterine growth occurred. Administration of estriol or estradiol as a pellet implant, which causes continuous occupancy of type I sites, increases the quantity of nuclear type II sites and stimulates true uterine growth. Therefore, we conclude that elevated levels of nuclear type II sites correlate with the long term uterotropic response to estrogenic hormones. Although we do not understand the function of this second class of binding sites it is possible that the type II sites represent a major component in the mechanism by which estrogens stimulate growth of the uterus."}
{"id": "PMID:474277", "title": "Regulation and function of estrogen and progesterone receptor systems.", "content": "We have developed and validated assay methods which are appropriate for studying the subcellular distribution of estrogen receptor (Re) and progesterone receptor (Rp) in hamster uterus during the estrous cycle and pregnancy. Cytosol receptors were monitored by conventional procedures, and nuclear receptors were extracted from nuclei at 2 C using 0.5 M KCl in buffer containing glycerol and measured by ligand exchange during incubation with [3H] progesterone (P) at 2 C for 24 h or [3H] estradiol-17 beta (E2) at 30 C for 1 h. The nuclear exchange assay described herein measures total receptor and also permits estimation of unlabeled steroid in the nuclear KCl extract. Nuclear Rp translocation was shown to be target-tissue specific, hormone specific, and dependent on P dosage under in vivo conditions. The duration of nuclear Rp retention was about 6 h following exogenous P treatment. During the estrous cycle, Re and Rp levels in uterine cytosol and nuclei varied in response to the changing pattern of E2 and P secretion. Our results document a positive relationship between serum E2, nuclear Re and cytosol Rp levels during the follicular phase of the cycle. Nuclear Rp remained low on cycle days 1--3, and increased abruptly on day 4 coincident with preovulatory P secretion and cytosol Rp depletion. Nuclear Rp translocation during the preovulatory period on cycle day 4 was associated with a marked decline in nuclear Re, and the latter was demonstrated to be a rapid (2--4 h) response to P action rather than serum E2 withdrawal. The negative effect of P on nuclear Re levels may represent a fundamental mechanism for P antagonism of E action. Cytosol and nuclear Rp levels in deciduomal and myometrial tissue were responsive to experimental alteration of serum P, indicating that nuclear Rp is maintained by a continuous process of P-induced Rp translocation. Of interest was the finding in pregnant animals that Re levels declined dramatically in deciduoma as compared to myometrium. The loss of cytosol and nuclear Re from deciduomal cells cannot be attributed to changes in serum E2 or P. Thus, the intracellular regulation of Re numbers may change during differentiation of certain target cells such as those found in the deciduoma.", "contents": "Regulation and function of estrogen and progesterone receptor systems. We have developed and validated assay methods which are appropriate for studying the subcellular distribution of estrogen receptor (Re) and progesterone receptor (Rp) in hamster uterus during the estrous cycle and pregnancy. Cytosol receptors were monitored by conventional procedures, and nuclear receptors were extracted from nuclei at 2 C using 0.5 M KCl in buffer containing glycerol and measured by ligand exchange during incubation with [3H] progesterone (P) at 2 C for 24 h or [3H] estradiol-17 beta (E2) at 30 C for 1 h. The nuclear exchange assay described herein measures total receptor and also permits estimation of unlabeled steroid in the nuclear KCl extract. Nuclear Rp translocation was shown to be target-tissue specific, hormone specific, and dependent on P dosage under in vivo conditions. The duration of nuclear Rp retention was about 6 h following exogenous P treatment. During the estrous cycle, Re and Rp levels in uterine cytosol and nuclei varied in response to the changing pattern of E2 and P secretion. Our results document a positive relationship between serum E2, nuclear Re and cytosol Rp levels during the follicular phase of the cycle. Nuclear Rp remained low on cycle days 1--3, and increased abruptly on day 4 coincident with preovulatory P secretion and cytosol Rp depletion. Nuclear Rp translocation during the preovulatory period on cycle day 4 was associated with a marked decline in nuclear Re, and the latter was demonstrated to be a rapid (2--4 h) response to P action rather than serum E2 withdrawal. The negative effect of P on nuclear Re levels may represent a fundamental mechanism for P antagonism of E action. Cytosol and nuclear Rp levels in deciduomal and myometrial tissue were responsive to experimental alteration of serum P, indicating that nuclear Rp is maintained by a continuous process of P-induced Rp translocation. Of interest was the finding in pregnant animals that Re levels declined dramatically in deciduoma as compared to myometrium. The loss of cytosol and nuclear Re from deciduomal cells cannot be attributed to changes in serum E2 or P. Thus, the intracellular regulation of Re numbers may change during differentiation of certain target cells such as those found in the deciduoma."}
{"id": "PMID:474278", "title": "Effects of progestins on the progesterone receptor in guinea pig uterus.", "content": "We examined the effects of progesterone and some synthetic progestins and other steroids on the physical properties of the progesterone receptor of guinea pig uterine cytosol and on the binding of the receptor by nuclei. Progestational potency seemed to correlate with the ability to keep the receptor in the 7S form and to prevent dissociation into smaller subunits. The rate of activation prior to nuclear binding was slower with steroids of increasing progestational activity. Therefore activation in vitro may be unrelated to biological activity. Concentration of the cytosol led to a decrease in the equilibrium association constant. The extent of the decrease was less with progesterone than with its metabolite, 5 alpha-pregnanedione. When cytosol and nuclei were incubated in the absence of ligand measureable progesterone receptor was bound by the nuclei. The uncomplexed nuclear receptor bound [3H]-progesterone of [3H]-R5020 rapidly at 0 degrees, but progesterone-receptor complexes exchanged [3H]-progestin slowly at 0 degrees. Progesterone increased the amount of nuclear receptor at concentrations of 10(-9) and 10(-8)M, but decreased binding at higher concentrations. 5 alpha-Pregnanedione had the same effect as progesterone, but other metabolites of progesterone that had little affinity for the 7S progesterone receptor in cytosol had no effect on nuclear binding at any concentration. Glucocorticoids, testosterone and estradiol-17 beta increased the nuclear binding of the progesterone receptor when present at concentration of 10(-8)M and greater.", "contents": "Effects of progestins on the progesterone receptor in guinea pig uterus. We examined the effects of progesterone and some synthetic progestins and other steroids on the physical properties of the progesterone receptor of guinea pig uterine cytosol and on the binding of the receptor by nuclei. Progestational potency seemed to correlate with the ability to keep the receptor in the 7S form and to prevent dissociation into smaller subunits. The rate of activation prior to nuclear binding was slower with steroids of increasing progestational activity. Therefore activation in vitro may be unrelated to biological activity. Concentration of the cytosol led to a decrease in the equilibrium association constant. The extent of the decrease was less with progesterone than with its metabolite, 5 alpha-pregnanedione. When cytosol and nuclei were incubated in the absence of ligand measureable progesterone receptor was bound by the nuclei. The uncomplexed nuclear receptor bound [3H]-progesterone of [3H]-R5020 rapidly at 0 degrees, but progesterone-receptor complexes exchanged [3H]-progestin slowly at 0 degrees. Progesterone increased the amount of nuclear receptor at concentrations of 10(-9) and 10(-8)M, but decreased binding at higher concentrations. 5 alpha-Pregnanedione had the same effect as progesterone, but other metabolites of progesterone that had little affinity for the 7S progesterone receptor in cytosol had no effect on nuclear binding at any concentration. Glucocorticoids, testosterone and estradiol-17 beta increased the nuclear binding of the progesterone receptor when present at concentration of 10(-8)M and greater."}
{"id": "PMID:474279", "title": "The use of medroxyprogesterone acetate to study progestin receptors in immature, pregnant, and adult rabbit uterus.", "content": "Medroxyprogesterone acetate (MPA)3, a potent synthetic progestin, was employed as the 3H-ligand to investigate the status of the rabbit uterine progestin receptor under various hormonal conditions. MPA dissociated slowly from the cytosol and nuclear progestin receptor obtained from ovariectomized, estrogen-primed rabbits. This slow rate of dissociation permitted a partial characterization of the receptors in pregnant and immature, unprimed rabbit uteri. High affinity progestin binding to 6--7 S and 4 S macromolecular species was detected in cytosol and nuclear extracts, respectively, from these animals. In contrast to the 3 peaks of cytosol receptor activity from estrogen-primed animals which were eluted stepwise from DEAE-cellulose columns, predominantly 2 peaks were obtained with cytosols from pregnant and immature, unprimed uteri. Further studies are required to determine to what extent progestin action is modified through interaction of these binding components with themselves and other cellular proteins.", "contents": "The use of medroxyprogesterone acetate to study progestin receptors in immature, pregnant, and adult rabbit uterus. Medroxyprogesterone acetate (MPA)3, a potent synthetic progestin, was employed as the 3H-ligand to investigate the status of the rabbit uterine progestin receptor under various hormonal conditions. MPA dissociated slowly from the cytosol and nuclear progestin receptor obtained from ovariectomized, estrogen-primed rabbits. This slow rate of dissociation permitted a partial characterization of the receptors in pregnant and immature, unprimed rabbit uteri. High affinity progestin binding to 6--7 S and 4 S macromolecular species was detected in cytosol and nuclear extracts, respectively, from these animals. In contrast to the 3 peaks of cytosol receptor activity from estrogen-primed animals which were eluted stepwise from DEAE-cellulose columns, predominantly 2 peaks were obtained with cytosols from pregnant and immature, unprimed uteri. Further studies are required to determine to what extent progestin action is modified through interaction of these binding components with themselves and other cellular proteins."}
{"id": "PMID:474280", "title": "Circannual rhythms in progesterone receptor levels and functions.", "content": "Studies in this laboratory on the nuclear binding sites (acceptors) for progesterone receptor in the developed chick oviduct have resulted in the detection of seasonal variations in the levels and functions of the receptor. Cytosol preparations obtained from the chick oviducts during the winter/spring period between January and May display reduced receptor levels as well as a loss of the capacity of the receptor to bind to nuclear \"acceptor\" sites in vitro. The binding of [3H]P-R to whole chromatin or purified acceptor proteins reannealed to DNA display the same rhythm. No such rhythm is detected for the binding of P-R to pure DNA. Computer analysis of the data, using least squares method to fit the data to cosine curves, shows a significant fit indicating a circannual rhythm in P-R binding to the acceptor protein-DNA complex but not to pure DNA. The nuclear binding in vivo, achieved by injecting [3H]progesterone into the wing vein and analyzing the radioactivity localized in the oviduct nuclei, also displays a similar rhythm. These results support that native nuclear acceptor sites for progesterone in the chick oviduct represent protein-DNA complexes and not pure DNA. The failure of P-R to bind the nuclear acceptor sites in vivo and in vitro during this period can be explained by the two subunit hypothesis of Schrader and O'Malley, whereby one of the two subunits is absent or inactive during this period.", "contents": "Circannual rhythms in progesterone receptor levels and functions. Studies in this laboratory on the nuclear binding sites (acceptors) for progesterone receptor in the developed chick oviduct have resulted in the detection of seasonal variations in the levels and functions of the receptor. Cytosol preparations obtained from the chick oviducts during the winter/spring period between January and May display reduced receptor levels as well as a loss of the capacity of the receptor to bind to nuclear \"acceptor\" sites in vitro. The binding of [3H]P-R to whole chromatin or purified acceptor proteins reannealed to DNA display the same rhythm. No such rhythm is detected for the binding of P-R to pure DNA. Computer analysis of the data, using least squares method to fit the data to cosine curves, shows a significant fit indicating a circannual rhythm in P-R binding to the acceptor protein-DNA complex but not to pure DNA. The nuclear binding in vivo, achieved by injecting [3H]progesterone into the wing vein and analyzing the radioactivity localized in the oviduct nuclei, also displays a similar rhythm. These results support that native nuclear acceptor sites for progesterone in the chick oviduct represent protein-DNA complexes and not pure DNA. The failure of P-R to bind the nuclear acceptor sites in vivo and in vitro during this period can be explained by the two subunit hypothesis of Schrader and O'Malley, whereby one of the two subunits is absent or inactive during this period."}
{"id": "PMID:474281", "title": "In vivo metabolism and binding of 6 alpha-methylprogesterone; a progestin with anti-androgenic and synandrogenic activities.", "content": "The synthetic progestin 6 alpha-methylprogesterone (6MP) was shown to have androgenic, synandrogenic and anti-androgenic actions on mouse kidney. In vitro studies indicated that 6MP competes with testosterone for binding sites on the androgen receptor in kidney cytosol and nuclei. The in vivo distribution of 6MP and testosterone differed following subcutaneous administration: Testosterone levels were 10 and 100-fold higher than those of 6MP in plasma and nuclei from prostate-seminal vesicle. In kidney nuclei, the concentrations of these two steroids were similar. In addition, kidney nuclei bound a hydroxylated metabolite of 6MP as well as the parent compound, whereas only 6MP was found in nuclei from prostate-seminal vesicle. In vivo competition studies indicated that nonradioactive 6MP and testosterone decreased the uptake of 3H-testosterone by nuclei of kidney and prostate-seminal vesicle. By contrast, 3H-6MP uptake in kidney nuclei was potentiated by the prior administration of 6MP or testosterone. These results suggest that factors in addition to the androgen receptor may be involved in the binding and mechanism of action of 6MP on mouse kidney.", "contents": "In vivo metabolism and binding of 6 alpha-methylprogesterone; a progestin with anti-androgenic and synandrogenic activities. The synthetic progestin 6 alpha-methylprogesterone (6MP) was shown to have androgenic, synandrogenic and anti-androgenic actions on mouse kidney. In vitro studies indicated that 6MP competes with testosterone for binding sites on the androgen receptor in kidney cytosol and nuclei. The in vivo distribution of 6MP and testosterone differed following subcutaneous administration: Testosterone levels were 10 and 100-fold higher than those of 6MP in plasma and nuclei from prostate-seminal vesicle. In kidney nuclei, the concentrations of these two steroids were similar. In addition, kidney nuclei bound a hydroxylated metabolite of 6MP as well as the parent compound, whereas only 6MP was found in nuclei from prostate-seminal vesicle. In vivo competition studies indicated that nonradioactive 6MP and testosterone decreased the uptake of 3H-testosterone by nuclei of kidney and prostate-seminal vesicle. By contrast, 3H-6MP uptake in kidney nuclei was potentiated by the prior administration of 6MP or testosterone. These results suggest that factors in addition to the androgen receptor may be involved in the binding and mechanism of action of 6MP on mouse kidney."}
{"id": "PMID:474283", "title": "The role of ligand-binding as a determinant of the structure and activation of the estrogen receptor.", "content": "The dissociation of estradiol from the estrogen receptor occurs in two kinetic phases: a fast component having a half-time of approximately 3 min and a slower, or second, dissociating component having a half-time of approximately 95 min at 28 degrees. The fast component is produced by the dissociation of estradiol from the nonactivated 4 S receptor, a monomer. Thus, the magnitude of the fast component of the [3H] estradiol biphasic dissociation curve is proportional to the fraction of the receptor in the nonactivated state. The slow component is the estradiol dissociating from the activated 5 S receptor, a dimer. The salt-extracted estrogen receptor isolated from uterine nuclei shows a single, slow dissociating component equal to the slower component of the cytoplasmic biphasic dissociation curve. Estradiol binding shifts the receptor equilibrium from the low affinity nonactivated 4 S receptor toward the high affinity activated 5 S receptor. The kinetics of estriol dissociation from the receptor shows a larger fractional magnitude for the fast component and a faster second dissociating component than estradiol. This suggests that estriol transforms a smaller fraction of the receptor to the activated state and that the activated estriol receptor has a shorter half-time than estradiol. The biphasic dissociation kinetics of an estrogen from the receptor provides a new and sensitive criterion for measuring receptor activation.", "contents": "The role of ligand-binding as a determinant of the structure and activation of the estrogen receptor. The dissociation of estradiol from the estrogen receptor occurs in two kinetic phases: a fast component having a half-time of approximately 3 min and a slower, or second, dissociating component having a half-time of approximately 95 min at 28 degrees. The fast component is produced by the dissociation of estradiol from the nonactivated 4 S receptor, a monomer. Thus, the magnitude of the fast component of the [3H] estradiol biphasic dissociation curve is proportional to the fraction of the receptor in the nonactivated state. The slow component is the estradiol dissociating from the activated 5 S receptor, a dimer. The salt-extracted estrogen receptor isolated from uterine nuclei shows a single, slow dissociating component equal to the slower component of the cytoplasmic biphasic dissociation curve. Estradiol binding shifts the receptor equilibrium from the low affinity nonactivated 4 S receptor toward the high affinity activated 5 S receptor. The kinetics of estriol dissociation from the receptor shows a larger fractional magnitude for the fast component and a faster second dissociating component than estradiol. This suggests that estriol transforms a smaller fraction of the receptor to the activated state and that the activated estriol receptor has a shorter half-time than estradiol. The biphasic dissociation kinetics of an estrogen from the receptor provides a new and sensitive criterion for measuring receptor activation."}
{"id": "PMID:474284", "title": "Structural relationships between the chick oviduct progesterone receptor A and B proteins.", "content": "The chick oviduct contains two distinct forms of the progesterone receptor, termed progestophilins A (Mr = 79,000) and B (Mr = 117,000). Although these two hormone-binding proteins differ significantly in physico-chemical characteristics, a good deal of similarity exists between these two molecules. Thus, both proteins display identical hormone-binding kinetics and steroid specificity. The hormone-binding fragments obtained after the action of an endogenous oviduct Ca2+-activated protease are indistinguishable as analyzed by gel filtration chromatography. Both the A and B proteins are capable of binding to DNA-cellulose, although they elute at very different salt concentrations when subjected to gradient elution. In spite of these similarities all attempts to demonstrate the conversion of progestophilin B to A or to show a common precursor to both have been unsuccessful. Therefore, either the A and B receptor proteins are separate products of closely related genes or the conversion of one form to another occurs very rapidly either in vivo or in vitro.", "contents": "Structural relationships between the chick oviduct progesterone receptor A and B proteins. The chick oviduct contains two distinct forms of the progesterone receptor, termed progestophilins A (Mr = 79,000) and B (Mr = 117,000). Although these two hormone-binding proteins differ significantly in physico-chemical characteristics, a good deal of similarity exists between these two molecules. Thus, both proteins display identical hormone-binding kinetics and steroid specificity. The hormone-binding fragments obtained after the action of an endogenous oviduct Ca2+-activated protease are indistinguishable as analyzed by gel filtration chromatography. Both the A and B proteins are capable of binding to DNA-cellulose, although they elute at very different salt concentrations when subjected to gradient elution. In spite of these similarities all attempts to demonstrate the conversion of progestophilin B to A or to show a common precursor to both have been unsuccessful. Therefore, either the A and B receptor proteins are separate products of closely related genes or the conversion of one form to another occurs very rapidly either in vivo or in vitro."}
{"id": "PMID:474285", "title": "Characterization of the avian progesterone receptor through the use of inhibitors.", "content": "Several chemical agents have been identified which block interaction of the avian progesterone receptor with isolated nuclei, ATP-Sepharose, DNA-cellulose or phosphocellulose. Four of these inhibitors, rifamycin AF/103, o-phenanthroline, aurintricarboxylic acid and pyridoxal 5-phosphate appear to block directly binding of the activated receptor complex to the above \"acceptors.\" Another inhibitor, sodium molybdate, only blocks receptor interactions when added before receptor activation and therefore appears to interfere with the activation process. When nuclear receptor complexes were formed in vivo and labeled by nuclear exchange with [3H]progesterone in vitro, these complexes could not be disrupted by incubation of the nuclei with inhibitors. Therefore, the receptor complex bound in nuclei appears to be modified or masked in a way which resists the action of these chemical agents. These results indicate the value of inhibitors as chemical probes for the analysis of steroid receptors.", "contents": "Characterization of the avian progesterone receptor through the use of inhibitors. Several chemical agents have been identified which block interaction of the avian progesterone receptor with isolated nuclei, ATP-Sepharose, DNA-cellulose or phosphocellulose. Four of these inhibitors, rifamycin AF/103, o-phenanthroline, aurintricarboxylic acid and pyridoxal 5-phosphate appear to block directly binding of the activated receptor complex to the above \"acceptors.\" Another inhibitor, sodium molybdate, only blocks receptor interactions when added before receptor activation and therefore appears to interfere with the activation process. When nuclear receptor complexes were formed in vivo and labeled by nuclear exchange with [3H]progesterone in vitro, these complexes could not be disrupted by incubation of the nuclei with inhibitors. Therefore, the receptor complex bound in nuclei appears to be modified or masked in a way which resists the action of these chemical agents. These results indicate the value of inhibitors as chemical probes for the analysis of steroid receptors."}
{"id": "PMID:474286", "title": "Glucocorticoid receptor cleavage by leupeptin-sensitive enzymes in rat kidney cytosol.", "content": "The proteolytic origin of small fragments of both the glucocorticoid and mineralocorticoid receptors in rat kidney cytosol was inferred from the effects of leupeptin, a bacterial tripeptide that inhibits many proteases [Sherman, M.R. et al., (1978). Federation Proc. 37:167--173]. In the present study, the smallest fragment of the glucocorticoid receptor containing the steroid-binding site, the mero-receptor, was characterized with respect to the Stokes radius (RS = 23 +/- 3 A) and the isoelectric point (pI = 5.9 at 4 degrees). Chromatography of cytosol labeled with [3H]triamcinolone acetonide on Sephadex LH-20 (Pharmacia) in aqueous buffer resolved the steroid-receptor complex from the unmodified free steroid and from steroid metabolites and contaminants. This technique facilitated analyses of the leupeptin-stabilized receptor form by isoelectric focusing (pU = 5.9 at 4 degrees) and centrifugation in glycerol gradients (s20,w = 9--11 S in 50mM KCl). When this large complex in fresh cytosol was analyzed on Agarose (Bio-Rad) at a high flow rate, it had RS congruent to 60 A in 50 mM KCl and RS congruent to 30 A in 400 mM KCl. These analytical studies with leupeptin indicate the need for inexpensive, irreversible inhibitors of proteolytic enzymes for the purification of intact receptors, holo-receptors, from kidney and other tissues. Specific proteases can then be applied to dissect the holo-receptor into the globular mero-receptor, proximal to the steroid-binding site, and the asymmetric region(s), distal segment(s), that may be involved in the nuclear interactions.", "contents": "Glucocorticoid receptor cleavage by leupeptin-sensitive enzymes in rat kidney cytosol. The proteolytic origin of small fragments of both the glucocorticoid and mineralocorticoid receptors in rat kidney cytosol was inferred from the effects of leupeptin, a bacterial tripeptide that inhibits many proteases [Sherman, M.R. et al., (1978). Federation Proc. 37:167--173]. In the present study, the smallest fragment of the glucocorticoid receptor containing the steroid-binding site, the mero-receptor, was characterized with respect to the Stokes radius (RS = 23 +/- 3 A) and the isoelectric point (pI = 5.9 at 4 degrees). Chromatography of cytosol labeled with [3H]triamcinolone acetonide on Sephadex LH-20 (Pharmacia) in aqueous buffer resolved the steroid-receptor complex from the unmodified free steroid and from steroid metabolites and contaminants. This technique facilitated analyses of the leupeptin-stabilized receptor form by isoelectric focusing (pU = 5.9 at 4 degrees) and centrifugation in glycerol gradients (s20,w = 9--11 S in 50mM KCl). When this large complex in fresh cytosol was analyzed on Agarose (Bio-Rad) at a high flow rate, it had RS congruent to 60 A in 50 mM KCl and RS congruent to 30 A in 400 mM KCl. These analytical studies with leupeptin indicate the need for inexpensive, irreversible inhibitors of proteolytic enzymes for the purification of intact receptors, holo-receptors, from kidney and other tissues. Specific proteases can then be applied to dissect the holo-receptor into the globular mero-receptor, proximal to the steroid-binding site, and the asymmetric region(s), distal segment(s), that may be involved in the nuclear interactions."}
{"id": "PMID:474287", "title": "Studies on the mechanism of estradiol uptake by rat uterine cells and on estradiol binding to uterine plasma membranes.", "content": "A method for the preparation of viable uterine cell suspensions is described. Using this system the kinetics of estradiol uptake were studied in order to asses whether the steroid enters uterine cells by passive diffusion (1) or protein mediated diffusion (2). The data presented show that a) the rates of estradiol entry are nonsaturable; b) temperature dependence of uptake kinetics gives a linear Arrhenius plot; c) E2-6-CMO-BSA does not inhibit 3H-E2 uptake; d) uterine plasma membranes do not contain specific estrogen binding sites. Thus, estrogen uptake occures by passive diffusion.", "contents": "Studies on the mechanism of estradiol uptake by rat uterine cells and on estradiol binding to uterine plasma membranes. A method for the preparation of viable uterine cell suspensions is described. Using this system the kinetics of estradiol uptake were studied in order to asses whether the steroid enters uterine cells by passive diffusion (1) or protein mediated diffusion (2). The data presented show that a) the rates of estradiol entry are nonsaturable; b) temperature dependence of uptake kinetics gives a linear Arrhenius plot; c) E2-6-CMO-BSA does not inhibit 3H-E2 uptake; d) uterine plasma membranes do not contain specific estrogen binding sites. Thus, estrogen uptake occures by passive diffusion."}
{"id": "PMID:474288", "title": "Heterogeneity of AtT-20 cell glucocorticoid binding sites: evidence for a membrane receptor.", "content": "In previous studies we have found that intact AtT-20 cells contained two glucocorticoid binding sites with distinctly different affinities and specificity. In this paper, the nature of these sites was investigated by studying glucocorticoid binding to cytosol and to plasma membranes isolated from AtT-20 mouse pituitary tumor cells. Plasma membrane vesicles were isolated from AtT-20 cells and found to take up alpha-aminoisobutyric acid, indicating that they were properly oriented and functionally intact. Corticosterone bound to these vesicle in a time- and temperature-dependent manner. The binding exhibited a glucocorticoid preference since non-glucocorticoids such as progesterone, testosterone or estradiol were unable to inhibit binding. In addition, binding specificity differed from that of the cytoplasmic receptor since the synthetic glucocorticoids were also ineffective competitors. The major inhibitors of binding were corticosterone greater than 11-dehydrocorticosterone greater than 11-ketoprogesterone greater than cortisol. In other complementary studies, AtT-20 cell cytosol was tested to determine whether heterogenous soluble sites exhibiting a preference for the natural vs the synthetic steroid could also be identified. We found that binding sites for both steroid classes were approximately similar in number, specificity and behavior on ion-exchange chromatography. We conclude that, in addition to a classical soluble cytoplasmic glucocorticoid receptor, AtT-20 cells contain plasma membrane glucocorticoid binding sites. The affinity and specificity of these sites for the natural ligand, corticosterone, suggest that they play an important role in the subcellular mechanism of glucocorticoid action.", "contents": "Heterogeneity of AtT-20 cell glucocorticoid binding sites: evidence for a membrane receptor. In previous studies we have found that intact AtT-20 cells contained two glucocorticoid binding sites with distinctly different affinities and specificity. In this paper, the nature of these sites was investigated by studying glucocorticoid binding to cytosol and to plasma membranes isolated from AtT-20 mouse pituitary tumor cells. Plasma membrane vesicles were isolated from AtT-20 cells and found to take up alpha-aminoisobutyric acid, indicating that they were properly oriented and functionally intact. Corticosterone bound to these vesicle in a time- and temperature-dependent manner. The binding exhibited a glucocorticoid preference since non-glucocorticoids such as progesterone, testosterone or estradiol were unable to inhibit binding. In addition, binding specificity differed from that of the cytoplasmic receptor since the synthetic glucocorticoids were also ineffective competitors. The major inhibitors of binding were corticosterone greater than 11-dehydrocorticosterone greater than 11-ketoprogesterone greater than cortisol. In other complementary studies, AtT-20 cell cytosol was tested to determine whether heterogenous soluble sites exhibiting a preference for the natural vs the synthetic steroid could also be identified. We found that binding sites for both steroid classes were approximately similar in number, specificity and behavior on ion-exchange chromatography. We conclude that, in addition to a classical soluble cytoplasmic glucocorticoid receptor, AtT-20 cells contain plasma membrane glucocorticoid binding sites. The affinity and specificity of these sites for the natural ligand, corticosterone, suggest that they play an important role in the subcellular mechanism of glucocorticoid action."}
{"id": "PMID:474289", "title": "Sex steroid binding proteins in non-mammalian vertebrates.", "content": "High affinity cytosolic and nuclear steroid binding macromolecules have been identified in steroid target tissues of representative amphibians, reptiles and birds. In addition to these traditional \"receptors\" evidence exists which indicates the presence of other steroid binding proteins, varying in affinity and specificity, in the cytosol of steroid targets (liver, oviduct) of these same species. The possible relationships between cytosolic binding proteins of differing affinity and specificity and their relevance to hormone action in non-mammalian species is discussed.", "contents": "Sex steroid binding proteins in non-mammalian vertebrates. High affinity cytosolic and nuclear steroid binding macromolecules have been identified in steroid target tissues of representative amphibians, reptiles and birds. In addition to these traditional \"receptors\" evidence exists which indicates the presence of other steroid binding proteins, varying in affinity and specificity, in the cytosol of steroid targets (liver, oviduct) of these same species. The possible relationships between cytosolic binding proteins of differing affinity and specificity and their relevance to hormone action in non-mammalian species is discussed."}
{"id": "PMID:474290", "title": "The ovalbumin gene: transcriptional regulation by estrogen.", "content": "De novo synthesis of RNA sequences corresponding to intervening as well as to structural sequences of the ovalbumin gene have been detected in isolated oviduct nuclei. Their presence in the nuclear transcripts and their time course of induction support the hypothesis that transcription of structural and intervening sequences of the natural ovalbumin gene are regulated by steroid hormones. These results are in agreement with out previous demonstration of high-molecular-weight species of ovalbumin RNA in nuclei that contain structural as well as intervening RNA sequences and are thus likely precursors to mature cytoplasmic mRNAov. Analysis of the size of in vivo nuclear RNA by gel electrophoresis under denaturing conditions, revealed that withdrawal of hormone depletes the level of high molecular weight ovalbumin RNA as well as that of nature mRNAov and that readministration of estrogen induces the accumulation of both species. These results are consistent also with transcriptional regulation of the ovalbumin gene. In addition, they rule out the possibility that the rapid accumulation of mature mRNAov after secondard stimulation results from processing of ovalbumin RNA precursors that might have been stored in the withdrawn oviduct. We conclude that steroid hormones exert a primary effect at the level of gene transcription. Following this event, a series of coordinated cellular responses may occur which involve RNA processing, mRNA transport to the cytoplasm, protein synthesis and mRNA degradation. The final consequence of this network of molecular reactions is the induced cellular function inherent to a specific steroid hormone.", "contents": "The ovalbumin gene: transcriptional regulation by estrogen. De novo synthesis of RNA sequences corresponding to intervening as well as to structural sequences of the ovalbumin gene have been detected in isolated oviduct nuclei. Their presence in the nuclear transcripts and their time course of induction support the hypothesis that transcription of structural and intervening sequences of the natural ovalbumin gene are regulated by steroid hormones. These results are in agreement with out previous demonstration of high-molecular-weight species of ovalbumin RNA in nuclei that contain structural as well as intervening RNA sequences and are thus likely precursors to mature cytoplasmic mRNAov. Analysis of the size of in vivo nuclear RNA by gel electrophoresis under denaturing conditions, revealed that withdrawal of hormone depletes the level of high molecular weight ovalbumin RNA as well as that of nature mRNAov and that readministration of estrogen induces the accumulation of both species. These results are consistent also with transcriptional regulation of the ovalbumin gene. In addition, they rule out the possibility that the rapid accumulation of mature mRNAov after secondard stimulation results from processing of ovalbumin RNA precursors that might have been stored in the withdrawn oviduct. We conclude that steroid hormones exert a primary effect at the level of gene transcription. Following this event, a series of coordinated cellular responses may occur which involve RNA processing, mRNA transport to the cytoplasm, protein synthesis and mRNA degradation. The final consequence of this network of molecular reactions is the induced cellular function inherent to a specific steroid hormone."}
{"id": "PMID:474291", "title": "The specific binding of estradiol to the nuclear matrix.", "content": "Recent studies on the eukaryotic nucleus have described the presence of a structural component termed the nuclear matrix, that appears to be involved in nucleic acid synthesis. This study will present evidence that the nuclear matrix of both the rat uterus and chick liver contains specific, high affinity, saturable estradiol binding sites.", "contents": "The specific binding of estradiol to the nuclear matrix. Recent studies on the eukaryotic nucleus have described the presence of a structural component termed the nuclear matrix, that appears to be involved in nucleic acid synthesis. This study will present evidence that the nuclear matrix of both the rat uterus and chick liver contains specific, high affinity, saturable estradiol binding sites."}
{"id": "PMID:474292", "title": "Heterogeneity of nuclear glucocorticoid receptor interactions.", "content": "When thymocytes are incubated with glucocorticoids at 37 degrees, 60--70% of the receptor bound steroid is associated with the nucleus. Under conditions where the rate of steroid-receptor formation is not limiting the transfer of steroid-receptors from the cytoplasm to the nucleus occurs rapidly with a T 1/2 of 30 seconds. These observations have led us to investigate whether or not all glucocorticoid receptor complexes are associated with the nucleus in the same manner. To this end, nuclear glucocorticoid-receptor complexes have been extracted by differential salt extraction and DNase I and DNase II digeston. Of the nuclear dexamethasone receptor complex initially bound, 70--75% is resistant to 0.2 M KCl extraction (designated N2) and 25--30% is resistant to 0.4 extraction (designated N4). N2 can be further extracted with 0.4 M KCl whereas N4 is resistant to reextraction with either 0.2 M KCl, suggesting that N2-N4 (N2-4) and N4 represent distinct physical forms of nuclear dexamethasone receptor. In intact cells, N2 and N4 differ under the following physiological condition. (1) N4 binding occurs prior to N2-4; (2) a cold chase of unlabeled dexamethasone decreases N2-4 by 70% but N4 binding by only 10%; (3) N4 binding decreases more rapidly than N2-4 following a decrease in hormone concentration by dilution; (4) a cold chase of either cortexolone or progesterone preferentially decreases N2-4 and has little effect on N4. In addition, the nuclear N2-4 and N4 distribution differ for cortisol, dexamethasone and triamcinolone acetonide, three steroids having different in vitro biological potencies. DNase I treatment of nuclei solubilizes approximately 60% of nuclear DNA yet releases only 20--30% of nuclear receptor, whereas DNase II solubilizes only 10% of nuclear DNA and releases 76--80% of nuclear receptor. As seen with salt extraction, the resistance of nuclear glucocorticoid-receptor complexes to a DNase I and II is dependent on the steroid molecule which is associated with the receptor. Of the steroids we have tested, nuclear triamcinolone acetonide and dexamethasone receptor complexes are most resistant to nuclease attack. Nuclear cortisol receptor complexes are readily solubilized by either DNase I or II under conditions where little dissociation of steroid from receptor occurs. These data represent evidence for physiologically distinct forms of nuclear glucocorticoid receptor interaction. In addition, they demonstrate the importance of the steroid portion of the steroid receptor in directing the nature and/or location of steroid receptors within or on the nucleus.", "contents": "Heterogeneity of nuclear glucocorticoid receptor interactions. When thymocytes are incubated with glucocorticoids at 37 degrees, 60--70% of the receptor bound steroid is associated with the nucleus. Under conditions where the rate of steroid-receptor formation is not limiting the transfer of steroid-receptors from the cytoplasm to the nucleus occurs rapidly with a T 1/2 of 30 seconds. These observations have led us to investigate whether or not all glucocorticoid receptor complexes are associated with the nucleus in the same manner. To this end, nuclear glucocorticoid-receptor complexes have been extracted by differential salt extraction and DNase I and DNase II digeston. Of the nuclear dexamethasone receptor complex initially bound, 70--75% is resistant to 0.2 M KCl extraction (designated N2) and 25--30% is resistant to 0.4 extraction (designated N4). N2 can be further extracted with 0.4 M KCl whereas N4 is resistant to reextraction with either 0.2 M KCl, suggesting that N2-N4 (N2-4) and N4 represent distinct physical forms of nuclear dexamethasone receptor. In intact cells, N2 and N4 differ under the following physiological condition. (1) N4 binding occurs prior to N2-4; (2) a cold chase of unlabeled dexamethasone decreases N2-4 by 70% but N4 binding by only 10%; (3) N4 binding decreases more rapidly than N2-4 following a decrease in hormone concentration by dilution; (4) a cold chase of either cortexolone or progesterone preferentially decreases N2-4 and has little effect on N4. In addition, the nuclear N2-4 and N4 distribution differ for cortisol, dexamethasone and triamcinolone acetonide, three steroids having different in vitro biological potencies. DNase I treatment of nuclei solubilizes approximately 60% of nuclear DNA yet releases only 20--30% of nuclear receptor, whereas DNase II solubilizes only 10% of nuclear DNA and releases 76--80% of nuclear receptor. As seen with salt extraction, the resistance of nuclear glucocorticoid-receptor complexes to a DNase I and II is dependent on the steroid molecule which is associated with the receptor. Of the steroids we have tested, nuclear triamcinolone acetonide and dexamethasone receptor complexes are most resistant to nuclease attack. Nuclear cortisol receptor complexes are readily solubilized by either DNase I or II under conditions where little dissociation of steroid from receptor occurs. These data represent evidence for physiologically distinct forms of nuclear glucocorticoid receptor interaction. In addition, they demonstrate the importance of the steroid portion of the steroid receptor in directing the nature and/or location of steroid receptors within or on the nucleus."}
{"id": "PMID:474293", "title": "Estrogen control of progesterone receptor induction in human breast cancer: role of nuclear estrogen receptor.", "content": "We have provocative data which suggest that the nuclear estrogen-receptor complex interacts with DNA, that this interaction is required for appropriate receptor turnover or processing, and that processing may be essential for induction of a specific protein by estrogen. If the receptor is improperly inserted into DNA, as for instance when it is bound by nafoxidine, processing fails and the biological effect is blunted.", "contents": "Estrogen control of progesterone receptor induction in human breast cancer: role of nuclear estrogen receptor. We have provocative data which suggest that the nuclear estrogen-receptor complex interacts with DNA, that this interaction is required for appropriate receptor turnover or processing, and that processing may be essential for induction of a specific protein by estrogen. If the receptor is improperly inserted into DNA, as for instance when it is bound by nafoxidine, processing fails and the biological effect is blunted."}
{"id": "PMID:474302", "title": "Electrophysiological studies on the effects of the neurotoxin apamin on cultured neurons.", "content": "Primary cultures of rat embryonic central nervous system were grown for 3 to 4 weeks in vitro. Impalement of neurons by means of high-resistance glass pipettes revealed that firing of action potentials could either be elicited by application of short square pulses or occurred spontaneously. The presence of the neurotoxin apamin caused a hyperpolarization of the membrane potential accompanied by an increase of the spike amplitude as well as an increase of membrane resistance. The significance of this effect is discussed in terms of a postsynaptic effect of this toxin.", "contents": "Electrophysiological studies on the effects of the neurotoxin apamin on cultured neurons. Primary cultures of rat embryonic central nervous system were grown for 3 to 4 weeks in vitro. Impalement of neurons by means of high-resistance glass pipettes revealed that firing of action potentials could either be elicited by application of short square pulses or occurred spontaneously. The presence of the neurotoxin apamin caused a hyperpolarization of the membrane potential accompanied by an increase of the spike amplitude as well as an increase of membrane resistance. The significance of this effect is discussed in terms of a postsynaptic effect of this toxin."}
{"id": "PMID:474303", "title": "A comparison of the carrageenan edema test and ultraviolet light-induced erythema test as predictors of the clinical dose in rheumatoid arthritis.", "content": "Twelve non-steroidal anti-inflammatory agents (NSAI's) and one steroidal anti-inflammatory agent, dexamethasone, were examined in the carrageenan edema test (CET) in the rat and in the ultraviolet light-induced erythema test (UVE) in the guinea pig to evaluate the correlation between those models of inflammation and the clinical dose of the NSAI's in the treatment of rheumatoid arthritis. The regression of the logarithm of the clinical dose with the logarithm of the ED50 for UVE gave a slope of 0.54 implying a non-parallelism of assays and a difference in mechanism. Dexamethasone failed to inhibit the UVE thereby corroborating this point. The parallelism of the logarithm of the clinical dose with the logarithm of the ED50 for the CET was substantially better (slope = 0.86). Dexamethasone was active in CET and its dose would be predicted by the CET regression. When only one variable was used for a prediction, log(CET) was a better predictor of log (clinical dose) than log(UVE). Standard methods for best regression selection indicated that even when both predictor variables were considered, log(CET) alone gave the best regression equation for predicting clinical dose. The view that inhibition of prostaglandin biosynthesis is the primary anti-inflammatory mechanism of NSAI's in rheumatoid arthritis is discussed in terms of these findings.", "contents": "A comparison of the carrageenan edema test and ultraviolet light-induced erythema test as predictors of the clinical dose in rheumatoid arthritis. Twelve non-steroidal anti-inflammatory agents (NSAI's) and one steroidal anti-inflammatory agent, dexamethasone, were examined in the carrageenan edema test (CET) in the rat and in the ultraviolet light-induced erythema test (UVE) in the guinea pig to evaluate the correlation between those models of inflammation and the clinical dose of the NSAI's in the treatment of rheumatoid arthritis. The regression of the logarithm of the clinical dose with the logarithm of the ED50 for UVE gave a slope of 0.54 implying a non-parallelism of assays and a difference in mechanism. Dexamethasone failed to inhibit the UVE thereby corroborating this point. The parallelism of the logarithm of the clinical dose with the logarithm of the ED50 for the CET was substantially better (slope = 0.86). Dexamethasone was active in CET and its dose would be predicted by the CET regression. When only one variable was used for a prediction, log(CET) was a better predictor of log (clinical dose) than log(UVE). Standard methods for best regression selection indicated that even when both predictor variables were considered, log(CET) alone gave the best regression equation for predicting clinical dose. The view that inhibition of prostaglandin biosynthesis is the primary anti-inflammatory mechanism of NSAI's in rheumatoid arthritis is discussed in terms of these findings."}
{"id": "PMID:474304", "title": "Studies on cell motility in inflammation. I. The chemotactic activity of experimental, immunological and non-immunological, inflammatory exudates.", "content": "The accumulation of leucocytes at the site of inflammation may be brought about by chemotaxis or proliferation in the extravascular tissues. The present paper focuses on the chemotactic properties of different types of experimental inflammatory pleural exudates, using a modified Boyden chamber. The time-course of carrageenan-induced exudate chemotactic activity for polymorphs was maximal at 4 h, thereafter diminishing towards 24 and 48 h. Chemotactic activity for mononuclear cells remained unchanged throughout the 4--48 h time-course. Heating the exudates to 56 degrees C for 1 h partially reduced chemotactic activity. These results correlate well with the migration of polymorph and mononuclear cells into the pleural cavity during carrageenan-induced pleurisy. The potency of polymorph and mononuclear cell chemotactic activity of different exudates was of the following order: carrageenan greater than calcium pyrophosphate greater than reverse passive Arthus greater than dextran. The results are discussed in order to elucidate the differences between the underlying mechanisms responsible for leucocyte accumulation in different types of inflammatory reaction.", "contents": "Studies on cell motility in inflammation. I. The chemotactic activity of experimental, immunological and non-immunological, inflammatory exudates. The accumulation of leucocytes at the site of inflammation may be brought about by chemotaxis or proliferation in the extravascular tissues. The present paper focuses on the chemotactic properties of different types of experimental inflammatory pleural exudates, using a modified Boyden chamber. The time-course of carrageenan-induced exudate chemotactic activity for polymorphs was maximal at 4 h, thereafter diminishing towards 24 and 48 h. Chemotactic activity for mononuclear cells remained unchanged throughout the 4--48 h time-course. Heating the exudates to 56 degrees C for 1 h partially reduced chemotactic activity. These results correlate well with the migration of polymorph and mononuclear cells into the pleural cavity during carrageenan-induced pleurisy. The potency of polymorph and mononuclear cell chemotactic activity of different exudates was of the following order: carrageenan greater than calcium pyrophosphate greater than reverse passive Arthus greater than dextran. The results are discussed in order to elucidate the differences between the underlying mechanisms responsible for leucocyte accumulation in different types of inflammatory reaction."}
{"id": "PMID:474305", "title": "Anti-inflammatory action following liver damage in the rat.", "content": "A high molecular weight protein fraction, obtained by column chromatography of saline extracts of the livers of rats that had previously been treated with dimethylnitrosamine, was found to have anti-inflammatory activity against carrageenin induced inflammation in the rat. Evidence was presented that the anti-inflammatory activity may be due to a protein fragment produced by proteolysis of a larger molecule.", "contents": "Anti-inflammatory action following liver damage in the rat. A high molecular weight protein fraction, obtained by column chromatography of saline extracts of the livers of rats that had previously been treated with dimethylnitrosamine, was found to have anti-inflammatory activity against carrageenin induced inflammation in the rat. Evidence was presented that the anti-inflammatory activity may be due to a protein fragment produced by proteolysis of a larger molecule."}
{"id": "PMID:474306", "title": "Anti-arthritic effect of bee venom.", "content": "Bee venom, administered subcutaneously, suppressed the development of carrageenan-induced paw edema and adjuvant arthritis in the rat in a dose-related manner. A single dose of bee venom administered subcutaneously the day before or on the day of injection of complete Freund's adjuvant (CFA) effectively suppressed the development of polyarthritis. This suppressive effect decreased progressively as dosing was delayed. Bee venom was found to be most effective when mixed and injected (sub-plantar) together with CFA, the disease-inducing agent. Similarly, antigens such as egg albumin, when incorporated into CFA, and injected into the hind paw, prevented the development of arthritis. These results suggest that at least two mechanisms are involved in the anti-arthritic action of bee venom: (1) alteration of the immune response, probably via antigen competition, and (2) an anti-inflammatory action via corticosteroids or through an as yet undetermined mechanism.", "contents": "Anti-arthritic effect of bee venom. Bee venom, administered subcutaneously, suppressed the development of carrageenan-induced paw edema and adjuvant arthritis in the rat in a dose-related manner. A single dose of bee venom administered subcutaneously the day before or on the day of injection of complete Freund's adjuvant (CFA) effectively suppressed the development of polyarthritis. This suppressive effect decreased progressively as dosing was delayed. Bee venom was found to be most effective when mixed and injected (sub-plantar) together with CFA, the disease-inducing agent. Similarly, antigens such as egg albumin, when incorporated into CFA, and injected into the hind paw, prevented the development of arthritis. These results suggest that at least two mechanisms are involved in the anti-arthritic action of bee venom: (1) alteration of the immune response, probably via antigen competition, and (2) an anti-inflammatory action via corticosteroids or through an as yet undetermined mechanism."}
{"id": "PMID:474307", "title": "Influence of vasodilators used in the therapy of heart failure on platelet aggregation.", "content": "Of the vasodilators used at present in the treatment of heart failure, only nitroprusside and phentolamine inhibit platelet aggregation at therapeutic dose levels. The other vasodilators studied, viz. nitroglycerin, isosorbide dinitrate, hydrallazine, dihydrallazine and prazosin, only inhibit platelet aggregation at relatively high concentrations, well above those reached in vivo. The exact nature of the platelet receptor, stimulation and blockade of which respectively initiate and inhibit aggregation, is not yet know, but it would appear to resemble the presynaptic alpha-receptors of other tissues.", "contents": "Influence of vasodilators used in the therapy of heart failure on platelet aggregation. Of the vasodilators used at present in the treatment of heart failure, only nitroprusside and phentolamine inhibit platelet aggregation at therapeutic dose levels. The other vasodilators studied, viz. nitroglycerin, isosorbide dinitrate, hydrallazine, dihydrallazine and prazosin, only inhibit platelet aggregation at relatively high concentrations, well above those reached in vivo. The exact nature of the platelet receptor, stimulation and blockade of which respectively initiate and inhibit aggregation, is not yet know, but it would appear to resemble the presynaptic alpha-receptors of other tissues."}
{"id": "PMID:474308", "title": "On the nephrotoxicity of alpha-amanitin and the antagonistic effects of silymarin in rats.", "content": "In human beings, poisoning by the deathcup mushroom causes renal lesions in addition to extremely severe liver damage. It is known from animal experiments that silymarin, a polyhydroxyphenylchromanone, is capable of counteracting this alpha-amanitin-induced liver damage. The purpose of the present work was to ascertain whether renal damage could be induced in rats by giving alpha-amanitin, and whether silymarin would have any effect on such renal damage. The fact that alpha-amanitin produces pathological changes in the kidneys and that these lesions can be almost completely prevented by pretreating rats with silymarin has now been amply demonstrated by biochemical and histological techniques alike.", "contents": "On the nephrotoxicity of alpha-amanitin and the antagonistic effects of silymarin in rats. In human beings, poisoning by the deathcup mushroom causes renal lesions in addition to extremely severe liver damage. It is known from animal experiments that silymarin, a polyhydroxyphenylchromanone, is capable of counteracting this alpha-amanitin-induced liver damage. The purpose of the present work was to ascertain whether renal damage could be induced in rats by giving alpha-amanitin, and whether silymarin would have any effect on such renal damage. The fact that alpha-amanitin produces pathological changes in the kidneys and that these lesions can be almost completely prevented by pretreating rats with silymarin has now been amply demonstrated by biochemical and histological techniques alike."}
{"id": "PMID:474322", "title": "Therapeutic measures in allergic rhinopathy cases resistent to conventional treatment.", "content": "If medical treatment fails to cure an allergic or vasomotor rhinopathy, we have to look for surgical procedures in trying to relieve the symptoms. Vidian neurectomy is a well known technique, but there still is much discussion about the technique and the long term results. With cryosurgery of the inferior nasal turbinates we obtain a fibrosis and shrinking of these hypertrophied tissues. The nitrous oxide cryosurgery unit we use relies on the Joule-Thomson effect. The specially designed cryoprobe for intranasal procedures is against the medial wall of the turbinate for 2 minutes with freezing to between -50 degrees and -60 degrees C. Thirty-seven patients entered our study. In vasomotor rhinopathy 17 out of 24 patients were completely free of symptoms for at least 3 months, while 4 were better. In allergic rhinopathy, the results are good but we did not reach this high number of completely cured patients. Most of the patients showed a relapse after 3 to 6 months. This is probably due to a regrowth of the autonomic nerve endings. We did not note side efects in the patients included in our study.", "contents": "Therapeutic measures in allergic rhinopathy cases resistent to conventional treatment. If medical treatment fails to cure an allergic or vasomotor rhinopathy, we have to look for surgical procedures in trying to relieve the symptoms. Vidian neurectomy is a well known technique, but there still is much discussion about the technique and the long term results. With cryosurgery of the inferior nasal turbinates we obtain a fibrosis and shrinking of these hypertrophied tissues. The nitrous oxide cryosurgery unit we use relies on the Joule-Thomson effect. The specially designed cryoprobe for intranasal procedures is against the medial wall of the turbinate for 2 minutes with freezing to between -50 degrees and -60 degrees C. Thirty-seven patients entered our study. In vasomotor rhinopathy 17 out of 24 patients were completely free of symptoms for at least 3 months, while 4 were better. In allergic rhinopathy, the results are good but we did not reach this high number of completely cured patients. Most of the patients showed a relapse after 3 to 6 months. This is probably due to a regrowth of the autonomic nerve endings. We did not note side efects in the patients included in our study."}
{"id": "PMID:474323", "title": "Analysis of some functions of maternal and cord blood lymphocytes.", "content": "Various immunological investigations were performed on lymphocytes from pregnant women at the time of delivery and on cord blood cells. The results thus obtained show that: -- An appreciable number of in vitro responses to various antigens is observed among the cord blood specimens. They correlate with a corresponding positive test with the maternal blood. -- The stimulability of maternal lymphocytes to PHA and Con-A is within normal limits, whereas cord blood lymphocytes respond poorly to these two mitogens. -- The percentage of rosette forming T cells is very low in the cord blood and normal in maternal blood. -- The MLC in maternal-cord blood co-cultured show a significant inhibitory effect due to cord blood cells distinct from maternal cells. -- Cord blood lymphocytes show a normal capacity to elaborate the two lymphocytes LIF and LMF. On the other hand, the capacity of maternal lymphocytes to synthetize LMF seems to be strongly impaired. -- An inhibitory factor for PHA-stimulated mitogenic response is present in maternal serum whereas it is absent in the cord serum. -- Maternal serum IgG is significantly decreased when compared to that of normal controls. The values for IgM, IgA and IgE are within normal ranges. The cord blood IgG is slightly higher than that of the mothers. The values for IgM, IgA and IgE are very low and irregularly present.", "contents": "Analysis of some functions of maternal and cord blood lymphocytes. Various immunological investigations were performed on lymphocytes from pregnant women at the time of delivery and on cord blood cells. The results thus obtained show that: -- An appreciable number of in vitro responses to various antigens is observed among the cord blood specimens. They correlate with a corresponding positive test with the maternal blood. -- The stimulability of maternal lymphocytes to PHA and Con-A is within normal limits, whereas cord blood lymphocytes respond poorly to these two mitogens. -- The percentage of rosette forming T cells is very low in the cord blood and normal in maternal blood. -- The MLC in maternal-cord blood co-cultured show a significant inhibitory effect due to cord blood cells distinct from maternal cells. -- Cord blood lymphocytes show a normal capacity to elaborate the two lymphocytes LIF and LMF. On the other hand, the capacity of maternal lymphocytes to synthetize LMF seems to be strongly impaired. -- An inhibitory factor for PHA-stimulated mitogenic response is present in maternal serum whereas it is absent in the cord serum. -- Maternal serum IgG is significantly decreased when compared to that of normal controls. The values for IgM, IgA and IgE are within normal ranges. The cord blood IgG is slightly higher than that of the mothers. The values for IgM, IgA and IgE are very low and irregularly present."}
{"id": "PMID:474324", "title": "Complement in hematological neoplasias.", "content": "Total hemolytic complement (CH50) and eight antigenic fractions of the complement system were determined in 30 patients with hematological neoplasias, distributed into the following groups: six cases of non-Hodgkin's lymphomas (NHL), seven cases of chronic lymphocytic leukemia (CLL), five cases of Hodgkin's disease (HD), seven cases of acute leukemia (AL), three cases of chronic myeloid leukemia (CML) and two cases of multiple myeloma (MM). CH50 was titred accordingly to a modification of the Kabat and Mayer method, C1q, C1s, C3, C4, C5, INHC1, C3A and properdin were determined with specific antisera by Manani and Laurell's techniques. The results obtained showed significant increase in CH50 above normal values in patients with HD, AL, and CML, especially the former, even in early stages. C1s was found to be increased in CML and AL, as well as C3 in CML. C4 is increased in CML and HD. C5 follows a course similar to C4, being also increased CLL and MM. C9 is increased in all groups, except NHL. A significant increase in C3A was found in NHL, HD and AL. There were no significant variations in C1s, INHC1 and properdin in any of the former groups. No correlation was found between clinical course and complement increase. The role of complement in neoplastic disease is discussed.", "contents": "Complement in hematological neoplasias. Total hemolytic complement (CH50) and eight antigenic fractions of the complement system were determined in 30 patients with hematological neoplasias, distributed into the following groups: six cases of non-Hodgkin's lymphomas (NHL), seven cases of chronic lymphocytic leukemia (CLL), five cases of Hodgkin's disease (HD), seven cases of acute leukemia (AL), three cases of chronic myeloid leukemia (CML) and two cases of multiple myeloma (MM). CH50 was titred accordingly to a modification of the Kabat and Mayer method, C1q, C1s, C3, C4, C5, INHC1, C3A and properdin were determined with specific antisera by Manani and Laurell's techniques. The results obtained showed significant increase in CH50 above normal values in patients with HD, AL, and CML, especially the former, even in early stages. C1s was found to be increased in CML and AL, as well as C3 in CML. C4 is increased in CML and HD. C5 follows a course similar to C4, being also increased CLL and MM. C9 is increased in all groups, except NHL. A significant increase in C3A was found in NHL, HD and AL. There were no significant variations in C1s, INHC1 and properdin in any of the former groups. No correlation was found between clinical course and complement increase. The role of complement in neoplastic disease is discussed."}
{"id": "PMID:474325", "title": "Bacterial immunotherapy in bronchial asthma.", "content": "In the treatment of bronchospastic states, primary bacterial bronchial asthma and asthmatic bronchitis, one of the most controversial aspects is the use of bacterial vaccines. In general, the treatment of these conditions is symptomatic, neglecting for the most part the existing bacterial allergy. At present, as a result of great advances in the immunotherapy of these disorders, the importance of an allergic response to bacteria in the etiology of these bronchopathies is beyond doubt. The subjects were 120 patients, 59 women and 51 men, with ages ranging from 7 to 73 years. Of this group, 21 were children less than 12 years old and all had a diagnosis of bronchial asthma or asthmatic bronchitis, with or without paranasal sinusitis, and exclusively of bacterial etiology. In order to study and evaluate the effectiveness of immunotherapy, a questionnaire, reproduced in fig. 1 was sent to the patients three years after the beginning of treatment. For this study, only the answers to this questionnaire were considered. Examination of the results shows that in bronchial disorders with bacterial allergy, treatment with bacterial vaccine achieves a 75% success rate. In the small group of children studied (a larger group is currently under investigation), the results are even more satisfactory, reaching 90.47%. It is noteworthy that the immediate reaction to the skin test was positive (++ to ++++) in 83.32% of the cases. Of the greatest importance also is the associated paranasal sinusitis in 83% of the cases, part of a sinobronchopulmonary syndrome which contributes substantially to this state of bacterial allergy. Significant side effects were found in 52.6% of the patients and consisted mainly of slight catarrhal symptoms, apparent at the beginning of treatment. At present, we have no doubt whatsoever that immunotherapy is much more valuable than prolonged symptomatic therapy in the treatment of primary bacterial bronchial asthma.", "contents": "Bacterial immunotherapy in bronchial asthma. In the treatment of bronchospastic states, primary bacterial bronchial asthma and asthmatic bronchitis, one of the most controversial aspects is the use of bacterial vaccines. In general, the treatment of these conditions is symptomatic, neglecting for the most part the existing bacterial allergy. At present, as a result of great advances in the immunotherapy of these disorders, the importance of an allergic response to bacteria in the etiology of these bronchopathies is beyond doubt. The subjects were 120 patients, 59 women and 51 men, with ages ranging from 7 to 73 years. Of this group, 21 were children less than 12 years old and all had a diagnosis of bronchial asthma or asthmatic bronchitis, with or without paranasal sinusitis, and exclusively of bacterial etiology. In order to study and evaluate the effectiveness of immunotherapy, a questionnaire, reproduced in fig. 1 was sent to the patients three years after the beginning of treatment. For this study, only the answers to this questionnaire were considered. Examination of the results shows that in bronchial disorders with bacterial allergy, treatment with bacterial vaccine achieves a 75% success rate. In the small group of children studied (a larger group is currently under investigation), the results are even more satisfactory, reaching 90.47%. It is noteworthy that the immediate reaction to the skin test was positive (++ to ++++) in 83.32% of the cases. Of the greatest importance also is the associated paranasal sinusitis in 83% of the cases, part of a sinobronchopulmonary syndrome which contributes substantially to this state of bacterial allergy. Significant side effects were found in 52.6% of the patients and consisted mainly of slight catarrhal symptoms, apparent at the beginning of treatment. At present, we have no doubt whatsoever that immunotherapy is much more valuable than prolonged symptomatic therapy in the treatment of primary bacterial bronchial asthma."}
{"id": "PMID:474331", "title": "The temporal dimension in the lives of retarded adults: a problem of interaction and intervention.", "content": "This paper describes an ethnographic study of temporality among a group of 32 mentally retarded adults. Data were collected by participant observers over a period of 18 months. The findings indicate that retarded individuals may experience and employ a notion of time that varies substantially from that of mainstream American time. This results in problems of interaction and intervention between professionals and these retarded adults. Sociological concepts are proposed for understanding these interactional problems.", "contents": "The temporal dimension in the lives of retarded adults: a problem of interaction and intervention. This paper describes an ethnographic study of temporality among a group of 32 mentally retarded adults. Data were collected by participant observers over a period of 18 months. The findings indicate that retarded individuals may experience and employ a notion of time that varies substantially from that of mainstream American time. This results in problems of interaction and intervention between professionals and these retarded adults. Sociological concepts are proposed for understanding these interactional problems."}
{"id": "PMID:474332", "title": "Egocentric speech: an adaptive function applied to developmental disabilities in occupational therapy.", "content": "This paper reviews egocentric speech as a theoretical postulate of change relevant to the practice of occupational therapy. Egocentric speech, an important adaptive behavior in normal development, designates the verbalizations that usually occur while a child between the ages of two and seven performs an adaptive task and thereby increases problem-solving abilities. The behavior is first discussed as it relates to the structures of assimilation and accommodation in Piaget's earlier sensorimotor stage, then as it appears in the preoperatory level of the concrete operations stage of intelligence. The importance of the behavior as a foundation for the inner language and thinking capacities of the adult is supported by the research of Soviet scientists Vygotsky and Luria. Finally, three case studies are presented in which egocentric speech is used in conjunction with problem-solving tasks in occupational therapy.", "contents": "Egocentric speech: an adaptive function applied to developmental disabilities in occupational therapy. This paper reviews egocentric speech as a theoretical postulate of change relevant to the practice of occupational therapy. Egocentric speech, an important adaptive behavior in normal development, designates the verbalizations that usually occur while a child between the ages of two and seven performs an adaptive task and thereby increases problem-solving abilities. The behavior is first discussed as it relates to the structures of assimilation and accommodation in Piaget's earlier sensorimotor stage, then as it appears in the preoperatory level of the concrete operations stage of intelligence. The importance of the behavior as a foundation for the inner language and thinking capacities of the adult is supported by the research of Soviet scientists Vygotsky and Luria. Finally, three case studies are presented in which egocentric speech is used in conjunction with problem-solving tasks in occupational therapy."}
{"id": "PMID:474326", "title": "The immunologic response to tobacco antigens in smokers. III. Type III hypersensitivity skin reactions and specific serum precipitins to four different tobacco extracts in patients suffering from coronary artery disease.", "content": "In a population of 70 patients suffering from coronary heart disease (49 smokers and 21 non-smokers) intradermal tests and specific precipitation tests with four different extracts of tobacco were performed. Skin tests with tobacco extracts were positive in 41 smokers and 7 nonsmokers. In both groups the immediate and delayed type reactions were weak and of rather doubtful specificity, while the late type reaction was usually more frequent and stronger in smokers (in 14 patients it was positive with all preparations). Specific precipitins against tobacco antigens were found in the sera of 13 smokers but never in nonsmokers. The positive precipitation test correlated well with the positive late skin reactions to tobacco extracts. Such correlation with Ag 1 was observed in 9 out of 13 patients, with Ag II in four cases, with Ag IV and Ag V in 2 and 2 patients respectively. The coincidence of strongly positive late skin reactions and positive precipitation tests with tobacco extracts seems to indicate that tobacco allergy really exists and resembles, in a number of patients with coronary artery disease, type III hypersensitivity reactions to tobacco antigens. It is postulated that immune complexes of precipitins and tobacco antigens may be formed in some patients and initiate pathological processes in coronary arteries leading to their occlusion.", "contents": "The immunologic response to tobacco antigens in smokers. III. Type III hypersensitivity skin reactions and specific serum precipitins to four different tobacco extracts in patients suffering from coronary artery disease. In a population of 70 patients suffering from coronary heart disease (49 smokers and 21 non-smokers) intradermal tests and specific precipitation tests with four different extracts of tobacco were performed. Skin tests with tobacco extracts were positive in 41 smokers and 7 nonsmokers. In both groups the immediate and delayed type reactions were weak and of rather doubtful specificity, while the late type reaction was usually more frequent and stronger in smokers (in 14 patients it was positive with all preparations). Specific precipitins against tobacco antigens were found in the sera of 13 smokers but never in nonsmokers. The positive precipitation test correlated well with the positive late skin reactions to tobacco extracts. Such correlation with Ag 1 was observed in 9 out of 13 patients, with Ag II in four cases, with Ag IV and Ag V in 2 and 2 patients respectively. The coincidence of strongly positive late skin reactions and positive precipitation tests with tobacco extracts seems to indicate that tobacco allergy really exists and resembles, in a number of patients with coronary artery disease, type III hypersensitivity reactions to tobacco antigens. It is postulated that immune complexes of precipitins and tobacco antigens may be formed in some patients and initiate pathological processes in coronary arteries leading to their occlusion."}
{"id": "PMID:474333", "title": "A question of professional boundaries: implications for educational programs.", "content": "During recent on-site visits a curriculum fieldwork coordinator learned that occupational therapy students were expected to use physical therapy modalities in the treatment of patients. Since the students had no academic preparation in the use of these modalities, the curriculum committee of the academic program was advised of the situation. Two academic occupational therapy programs collaborated in sending out a 48-item questionnaire to the centers currently cooperating in their fieldwork programs with the purpose of determining the kinds of modalities being used in treatment. Results of the survey showed that there was self-reported use of physical therapy modalities by occupational therapists throughout the country with greater use in some regions. The survey also showed occupational therapy involvement in new treatment areas but diminished use of some traditional therapeutic media. Guidelines are suggested regarding student involvement in new treatment areas.", "contents": "A question of professional boundaries: implications for educational programs. During recent on-site visits a curriculum fieldwork coordinator learned that occupational therapy students were expected to use physical therapy modalities in the treatment of patients. Since the students had no academic preparation in the use of these modalities, the curriculum committee of the academic program was advised of the situation. Two academic occupational therapy programs collaborated in sending out a 48-item questionnaire to the centers currently cooperating in their fieldwork programs with the purpose of determining the kinds of modalities being used in treatment. Results of the survey showed that there was self-reported use of physical therapy modalities by occupational therapists throughout the country with greater use in some regions. The survey also showed occupational therapy involvement in new treatment areas but diminished use of some traditional therapeutic media. Guidelines are suggested regarding student involvement in new treatment areas."}
{"id": "PMID:474334", "title": "Olfaction and muscle activity: an EMG pilot study.", "content": "The therapeutic significance of limbic system structures in general, and olfactory structures specifically, has been described by Fox, Ayres, Moore, and Farber in their professional presentations and publications. No data have yet been provided to substantiate claims of the effects of olfactory stimulation on muscle activity. Effects of olfactory stimulation on human muscle activity at rest were studied in seven normal children and seven normal adults. Upper trapezius, biceps brachii, rectus abdominis, and rectus femoris were monitored electromyographically for changes in muscle action potentials using peppermint oil and smelling salts in each of three positions--supine, seated quietly, and standing. Trapezius showed the greatest responsiveness in relation to other muscles. Using peppermint oil, only 18 out of 168 or 10.7 percent of the EMG recordings showed changes in muscle activity. Using smelling salts, only 21 out of 168 or 12.5 percent of the EMG recordings showed changes in muscle activity. Directional effects of peppermint oil could not be predicted, whereas stimulation with smelling salts, when effective, generally produced increased muscle activity. Children were more responsive to olfactory agents than were adults. Sex of subjects did not seem to effect the distribution of responses. When smelling salts was presented as an initial stimulus, subjects showed a greater responsiveness to all stimuli. The present data indicate that proposed effects of olfactory stimulation are more limited than was previously believed. Possible implications for clinical practice and suggestions for further study are presented.", "contents": "Olfaction and muscle activity: an EMG pilot study. The therapeutic significance of limbic system structures in general, and olfactory structures specifically, has been described by Fox, Ayres, Moore, and Farber in their professional presentations and publications. No data have yet been provided to substantiate claims of the effects of olfactory stimulation on muscle activity. Effects of olfactory stimulation on human muscle activity at rest were studied in seven normal children and seven normal adults. Upper trapezius, biceps brachii, rectus abdominis, and rectus femoris were monitored electromyographically for changes in muscle action potentials using peppermint oil and smelling salts in each of three positions--supine, seated quietly, and standing. Trapezius showed the greatest responsiveness in relation to other muscles. Using peppermint oil, only 18 out of 168 or 10.7 percent of the EMG recordings showed changes in muscle activity. Using smelling salts, only 21 out of 168 or 12.5 percent of the EMG recordings showed changes in muscle activity. Directional effects of peppermint oil could not be predicted, whereas stimulation with smelling salts, when effective, generally produced increased muscle activity. Children were more responsive to olfactory agents than were adults. Sex of subjects did not seem to effect the distribution of responses. When smelling salts was presented as an initial stimulus, subjects showed a greater responsiveness to all stimuli. The present data indicate that proposed effects of olfactory stimulation are more limited than was previously believed. Possible implications for clinical practice and suggestions for further study are presented."}
{"id": "PMID:474328", "title": "Precipitating antibodies in sera of normal blood donors against some antigens related to type III allergy, detected by counterimmunoelectrophoresis.", "content": "Precipitating antibodies against five antigens, staphylococcus aureus, candida albicans, cotton wool dust, house dust and tetanus toxoid, related to type III allergy (Arthus reaction) were estimated in 100 sera of normal blood donors by the counterimmunoelectrophoresis technique. The three antigens staphylococcus aureus, candida and cotton wool dust exhibited rather high titres of precipitins in sera of normal blood donors, whereas nearly 80% of the sera yielded no precipitins against house dust and tetanus toxoid. For diagnostic purposes the borderline titres were estimated as follows: 1:8 for staphylococcus aureus, 1:16 for candida albicans and 1:8 for cotton wool dust. Even low titres with the house dust antigen indicated a pathogenic situation.", "contents": "Precipitating antibodies in sera of normal blood donors against some antigens related to type III allergy, detected by counterimmunoelectrophoresis. Precipitating antibodies against five antigens, staphylococcus aureus, candida albicans, cotton wool dust, house dust and tetanus toxoid, related to type III allergy (Arthus reaction) were estimated in 100 sera of normal blood donors by the counterimmunoelectrophoresis technique. The three antigens staphylococcus aureus, candida and cotton wool dust exhibited rather high titres of precipitins in sera of normal blood donors, whereas nearly 80% of the sera yielded no precipitins against house dust and tetanus toxoid. For diagnostic purposes the borderline titres were estimated as follows: 1:8 for staphylococcus aureus, 1:16 for candida albicans and 1:8 for cotton wool dust. Even low titres with the house dust antigen indicated a pathogenic situation."}
{"id": "PMID:474330", "title": "Insects as inhalant allergens.", "content": "The importance of insect dust as inhalant allergen (e.g. locust, cockroach, mite, butterfly, bee, fly, etc.) is frequently underestimated as a cause of allergic conjunctivitis, rhinitis and bronchial asthma. The high allergenic potency of insect dust is demonstrated in cases of occupational allergy against locust. This dust is an obligatory sensitizing agent depending upon the degree of exposure and the potency of the allergen irrespective of constitutional factors. No doubts exist any more about the importance of house-dust mite (Dermatophagoides pteronyssinus) as an inhalative allergen. Allergy analysis should be extended also to these insect allergens especially in unclarified cases of perennial and particularly seasonal bronchial asthma or its \"equivalents\" (atopic rhinitis and conjunctivitis).", "contents": "Insects as inhalant allergens. The importance of insect dust as inhalant allergen (e.g. locust, cockroach, mite, butterfly, bee, fly, etc.) is frequently underestimated as a cause of allergic conjunctivitis, rhinitis and bronchial asthma. The high allergenic potency of insect dust is demonstrated in cases of occupational allergy against locust. This dust is an obligatory sensitizing agent depending upon the degree of exposure and the potency of the allergen irrespective of constitutional factors. No doubts exist any more about the importance of house-dust mite (Dermatophagoides pteronyssinus) as an inhalative allergen. Allergy analysis should be extended also to these insect allergens especially in unclarified cases of perennial and particularly seasonal bronchial asthma or its \"equivalents\" (atopic rhinitis and conjunctivitis)."}
{"id": "PMID:474337", "title": "Activity guidelines for early subacute and high-risk cardiac patients.", "content": "Research in exercise physiology indicates that energy cost can only be used as a rough index in the selection of activity for cardiac patients because it does not accurately predict the cardiac stress associated with any given activity for any given individual. A framework is presented in this paper for occupational therapy assessment of function in the cardiac patient based on determination of cardiac stress with activity through skilled clinical observation employing telemetry EKG, auscultation, and blood pressure recording. Clear activity guidelines are then formulated, using a scientific data base and including modification of activity to reduce cardiac stress. The procedure for skilled clinical observation of function in cardiac patients is described and data are presented from the use of this procedure to evaluate early subacute and high-risk cardiac patients during self-care in an acute hospital.", "contents": "Activity guidelines for early subacute and high-risk cardiac patients. Research in exercise physiology indicates that energy cost can only be used as a rough index in the selection of activity for cardiac patients because it does not accurately predict the cardiac stress associated with any given activity for any given individual. A framework is presented in this paper for occupational therapy assessment of function in the cardiac patient based on determination of cardiac stress with activity through skilled clinical observation employing telemetry EKG, auscultation, and blood pressure recording. Clear activity guidelines are then formulated, using a scientific data base and including modification of activity to reduce cardiac stress. The procedure for skilled clinical observation of function in cardiac patients is described and data are presented from the use of this procedure to evaluate early subacute and high-risk cardiac patients during self-care in an acute hospital."}
{"id": "PMID:474338", "title": "Learning patterns of young children with above-elbow prostheses.", "content": "In order to develop an improved method of teaching young child amputees to operate the forearm lift and elbow lock of a conventional above-elbow prosthesis, learning patterns of 12 young above-elbow amputees were observed and described by occupational therapists. From these observations, signs of readiness for learning were identified and a method was proposed for teaching these controls to children. The therapist gave the child physical assistance as well as verbal instruction and a demonstration for each part of the operation and then for the combined motion. Application to activities required development of new habit patterns and appeared to be related to cognitive abilities beyond the sensorimotor level. Mechanical features of the double cycle lock tended to complicate learning and demonstrated the need to develop an elbow lock mechanism that better meets the needs of children.", "contents": "Learning patterns of young children with above-elbow prostheses. In order to develop an improved method of teaching young child amputees to operate the forearm lift and elbow lock of a conventional above-elbow prosthesis, learning patterns of 12 young above-elbow amputees were observed and described by occupational therapists. From these observations, signs of readiness for learning were identified and a method was proposed for teaching these controls to children. The therapist gave the child physical assistance as well as verbal instruction and a demonstration for each part of the operation and then for the combined motion. Application to activities required development of new habit patterns and appeared to be related to cognitive abilities beyond the sensorimotor level. Mechanical features of the double cycle lock tended to complicate learning and demonstrated the need to develop an elbow lock mechanism that better meets the needs of children."}
{"id": "PMID:474339", "title": "The use of life review activity with confused nursing home residents.", "content": "Reminiscing and life review are adaptive tasks of old age. This article describes an occupational therapy group program for confused nursing home residents designed to facilitate reminiscing and promote the review process. Results of the study indicate that behaviors of confused residents improved during group meetings and that participants who attended most frequently showed the greatest change in behavior.", "contents": "The use of life review activity with confused nursing home residents. Reminiscing and life review are adaptive tasks of old age. This article describes an occupational therapy group program for confused nursing home residents designed to facilitate reminiscing and promote the review process. Results of the study indicate that behaviors of confused residents improved during group meetings and that participants who attended most frequently showed the greatest change in behavior."}
{"id": "PMID:474340", "title": "Comparison of three positions for evaluating the asymmetrical tonic neck reflex.", "content": "Twenty-four males ages 8 to 11 were videotaped while being tested in three positions for the ATNR: quadruped with the head passively rotated to either side; standing with the arms outstretched and eyes closed with the head passively rotated to either side; and quadruped reflex inhibiting posture with the skull hand on the hip and the face knee off the floor and with the head actively rotated to either side. Three \"expert\" raters viewed the edited tape and rated each subject in each position using a protocol devised for the study. The results suggest that the quadruped reflex inhibiting posture more readily reveals the presence of the reflex when compared to the other positions, in this population of learning-disabled children.", "contents": "Comparison of three positions for evaluating the asymmetrical tonic neck reflex. Twenty-four males ages 8 to 11 were videotaped while being tested in three positions for the ATNR: quadruped with the head passively rotated to either side; standing with the arms outstretched and eyes closed with the head passively rotated to either side; and quadruped reflex inhibiting posture with the skull hand on the hip and the face knee off the floor and with the head actively rotated to either side. Three \"expert\" raters viewed the edited tape and rated each subject in each position using a protocol devised for the study. The results suggest that the quadruped reflex inhibiting posture more readily reveals the presence of the reflex when compared to the other positions, in this population of learning-disabled children."}
{"id": "PMID:474341", "title": "Association between nystagmus hyporesponsivity and behavioral problems in learning-disabled children.", "content": "This study explored the hypothesis that an association exists between postrotary nystagmus hyporesponsivity and behavioral problems in learning-disabled children. Supporting this conclusion was the finding that the learning-disabled boys rated by teachers as displaying the most socially inappropriate behaviors had significantly lower postrotary nystagmus durations than other learning-disabled boys. In addition, near significant associations were obtained between subnormal nystagmus functioning and socially inappropriate responding for boys and girls combined. Learning-disabled girls were evaluated as responding significantly more appropriately than learning-disabled boys, while having significantly shorter postrotary nystagmus durations; thus, sex of a child may be an important variable in determining relationships between psychological characteristics and hyporesponsive nystagmus. Additional research is needed in this area because of a number of limitations to this study; however, these results present further suggestive evidence that learning-disabled children can be categorized according to characteristics of their postrotary nystagmus.", "contents": "Association between nystagmus hyporesponsivity and behavioral problems in learning-disabled children. This study explored the hypothesis that an association exists between postrotary nystagmus hyporesponsivity and behavioral problems in learning-disabled children. Supporting this conclusion was the finding that the learning-disabled boys rated by teachers as displaying the most socially inappropriate behaviors had significantly lower postrotary nystagmus durations than other learning-disabled boys. In addition, near significant associations were obtained between subnormal nystagmus functioning and socially inappropriate responding for boys and girls combined. Learning-disabled girls were evaluated as responding significantly more appropriately than learning-disabled boys, while having significantly shorter postrotary nystagmus durations; thus, sex of a child may be an important variable in determining relationships between psychological characteristics and hyporesponsive nystagmus. Additional research is needed in this area because of a number of limitations to this study; however, these results present further suggestive evidence that learning-disabled children can be categorized according to characteristics of their postrotary nystagmus."}
{"id": "PMID:474342", "title": "The quality of care in occupational therapy: an assessment of selected Michigan hospitals.", "content": "In this study, a methodology was developed and tested for assessing the quality of care in occupational therapy between educational and noneducational clinical settings, as measured by process and outcome. An instrument was constructed for an external audit of the hospital record. Standards drafted by the investigator were established as normative by a panel of experts for use in judging the programs. Hospital records of 84 patients with residual hemiparesis or hemiplegia in three noneducational settings and of 100 patients with similar diagnoses in two educational clinical settings from selected Michigan facilities were chosen by proportionate stratified random sampling. The process study showed that occupational therapy was of significantly higher quality in the educational settings. The outcome study did not show significant differences between types of settings. Implications for education and practice are discussed.", "contents": "The quality of care in occupational therapy: an assessment of selected Michigan hospitals. In this study, a methodology was developed and tested for assessing the quality of care in occupational therapy between educational and noneducational clinical settings, as measured by process and outcome. An instrument was constructed for an external audit of the hospital record. Standards drafted by the investigator were established as normative by a panel of experts for use in judging the programs. Hospital records of 84 patients with residual hemiparesis or hemiplegia in three noneducational settings and of 100 patients with similar diagnoses in two educational clinical settings from selected Michigan facilities were chosen by proportionate stratified random sampling. The process study showed that occupational therapy was of significantly higher quality in the educational settings. The outcome study did not show significant differences between types of settings. Implications for education and practice are discussed."}
{"id": "PMID:474345", "title": "Feedback goniometers for hand rehabilitation.", "content": "Active range-of-motion exercises and related activities are an important means for applying progressive stress to induce remodeling of various tissues that restrict range of motion in the hand and wrist. Supplying joint angle feedback to patients as they exercise can help them more consistently to reach the flexion/extension goals set by the therapist. To accomplish this aim, feedback goniometers have been developed that are worn on the hand during exercise and therapeutic activities. These devices supply a threshold feedback signal when a predetermined joint angle is attained. Clinical trials indicate that many patients in a comprehensive hand rehabilitation program after injuries or corrective surgery will improve somewhat more in active range of motion if they use feedback goniometers while exercising. Plans are available for fabricating such devices from readily obtained tools and parts at a modest cost.", "contents": "Feedback goniometers for hand rehabilitation. Active range-of-motion exercises and related activities are an important means for applying progressive stress to induce remodeling of various tissues that restrict range of motion in the hand and wrist. Supplying joint angle feedback to patients as they exercise can help them more consistently to reach the flexion/extension goals set by the therapist. To accomplish this aim, feedback goniometers have been developed that are worn on the hand during exercise and therapeutic activities. These devices supply a threshold feedback signal when a predetermined joint angle is attained. Clinical trials indicate that many patients in a comprehensive hand rehabilitation program after injuries or corrective surgery will improve somewhat more in active range of motion if they use feedback goniometers while exercising. Plans are available for fabricating such devices from readily obtained tools and parts at a modest cost."}
{"id": "PMID:474360", "title": "Aspirated foreign bodies in children.", "content": "Children six months to four years of age are at greatest risk for aspiration of foreign bodies. A history of coughing after contact with small objects is the most important clue. The clinical presentation varies from paroxysms of choking and coughing to chronic cough or wheezing. Chest radiographs are normal in up to one-half of the children. The location and size of the foreign body determine the urgency and selection of therapy. Prevention and a high index of suspicion will decrease morbidity.", "contents": "Aspirated foreign bodies in children. Children six months to four years of age are at greatest risk for aspiration of foreign bodies. A history of coughing after contact with small objects is the most important clue. The clinical presentation varies from paroxysms of choking and coughing to chronic cough or wheezing. Chest radiographs are normal in up to one-half of the children. The location and size of the foreign body determine the urgency and selection of therapy. Prevention and a high index of suspicion will decrease morbidity."}
{"id": "PMID:474361", "title": "Superimposed encephalopathy in the psychotic patient.", "content": "Encephalopathy (acute organic brain syndrome) superimposed on functional psychosis may be overlooked because the physician assumes that any exacerbation is due to fluctuation in the psychosis. Correct diagnosis requires a high index of suspicion and a firm knowledge of the signs of encephalopathy. A careful diagnostic approach permits the recognition of encephalopathy and separates it from functional disorders such as schizophrenia.", "contents": "Superimposed encephalopathy in the psychotic patient. Encephalopathy (acute organic brain syndrome) superimposed on functional psychosis may be overlooked because the physician assumes that any exacerbation is due to fluctuation in the psychosis. Correct diagnosis requires a high index of suspicion and a firm knowledge of the signs of encephalopathy. A careful diagnostic approach permits the recognition of encephalopathy and separates it from functional disorders such as schizophrenia."}
{"id": "PMID:474371", "title": "Current heart valve prostheses.", "content": "Current heart valve prostheses may be classified as either mechanical or tissue valves (bioprostheses). The principal advantage of mechanical devices is their established long-term durability; however, chronic anticoagulation is recommended to reduce the incidence of thromboembolic complications. Tissue valves are associated with a relatively low rate of thromboembolism but their durability level has not been determined. All contemporary prosthetic heart valves yield comparable survival rates.", "contents": "Current heart valve prostheses. Current heart valve prostheses may be classified as either mechanical or tissue valves (bioprostheses). The principal advantage of mechanical devices is their established long-term durability; however, chronic anticoagulation is recommended to reduce the incidence of thromboembolic complications. Tissue valves are associated with a relatively low rate of thromboembolism but their durability level has not been determined. All contemporary prosthetic heart valves yield comparable survival rates."}
{"id": "PMID:474373", "title": "Predicting results of coronary angiography.", "content": "This study compares the ability of various risk factor combinations to predict the extent of coronary artery disease found on coronary angiography. Risk factors were measured in 99 patients prior to coronary angiography. Clinical, epidemiological, psychosomatic, and combined orientations were compared as to their ability to predict angiography results. The clinical orientation was the most successful in predicting vessel disease (p less than .0001), followed by the epidemiological model, which was also successful (p less than .03). In contrast, psychosomatic factors were not accurate predictors of vessel disease. By combining all of the orientations, the accuracy of prediction is improved.", "contents": "Predicting results of coronary angiography. This study compares the ability of various risk factor combinations to predict the extent of coronary artery disease found on coronary angiography. Risk factors were measured in 99 patients prior to coronary angiography. Clinical, epidemiological, psychosomatic, and combined orientations were compared as to their ability to predict angiography results. The clinical orientation was the most successful in predicting vessel disease (p less than .0001), followed by the epidemiological model, which was also successful (p less than .03). In contrast, psychosomatic factors were not accurate predictors of vessel disease. By combining all of the orientations, the accuracy of prediction is improved."}
{"id": "PMID:474374", "title": "Pervenous retrieval of embolized catheters from the right heart and pulmonary arteries.", "content": "Successful removal of embolized or retained catheter fragments from the right heart was achieved in two out of four patients using pervenous catheter techniques. For the first time a fragment, radiolucent on image intensification, was retrieved from the right pulmonary artery using a wire snare. In a second case a hook-loop was made in the right ventricle with a Judkins left femoral coronary angiographic catheter, which has advantages over previously described hooking devices, to withdraw a fragment to the iliac vein for subsequent snaring. Failure of retrieval occurred only in specially difficult circumstances; when a catheter embolized to the pulmonary artery of a Tetralogy of Fallot, and when in spite of successful ensnarement, a fractured electrode was firmly adherent to the right ventricular apex. Successful pervenous retrieval may require a combination of techniques which move or dislodge, such as a hook or balloon combined with those which ensnare, such as a wire loop or bioptome. Using such techniques, with minimal additions to standard equipment, retrieval procedures can be offered as a routine cardiac catheterization service with a high rate of success.", "contents": "Pervenous retrieval of embolized catheters from the right heart and pulmonary arteries. Successful removal of embolized or retained catheter fragments from the right heart was achieved in two out of four patients using pervenous catheter techniques. For the first time a fragment, radiolucent on image intensification, was retrieved from the right pulmonary artery using a wire snare. In a second case a hook-loop was made in the right ventricle with a Judkins left femoral coronary angiographic catheter, which has advantages over previously described hooking devices, to withdraw a fragment to the iliac vein for subsequent snaring. Failure of retrieval occurred only in specially difficult circumstances; when a catheter embolized to the pulmonary artery of a Tetralogy of Fallot, and when in spite of successful ensnarement, a fractured electrode was firmly adherent to the right ventricular apex. Successful pervenous retrieval may require a combination of techniques which move or dislodge, such as a hook or balloon combined with those which ensnare, such as a wire loop or bioptome. Using such techniques, with minimal additions to standard equipment, retrieval procedures can be offered as a routine cardiac catheterization service with a high rate of success."}
{"id": "PMID:474376", "title": "Incidence of mitral valve prolapse in one hundred clinically stable newborn baby girls: an echocardiographic study.", "content": "Clinical and echocardiographic examinations were performed on 100 clinically stable, newborn baby girls. Mitral valve prolapse was noted on the echocardiograms of seven babies. Three subjects had systolic clicks, two of whom had systolic murmurs following the click. The four other babies who had echocardiographic evidence of mitral valve prolapse had no abnormal auscultatory signs. Of the 93 babies without evidence of mitral prolapse, 91 had normal echocardiograms and auscultatory features; one was noted to have a murmur consistent with a ventricular septal defect, and another had an eccentric aortic valve on the echocardiogram which was suggestive of a bicuspid aortic valve. Serial studies on our group of subjects will yield useful information regarding the natural history of mitral valve prolapse.", "contents": "Incidence of mitral valve prolapse in one hundred clinically stable newborn baby girls: an echocardiographic study. Clinical and echocardiographic examinations were performed on 100 clinically stable, newborn baby girls. Mitral valve prolapse was noted on the echocardiograms of seven babies. Three subjects had systolic clicks, two of whom had systolic murmurs following the click. The four other babies who had echocardiographic evidence of mitral valve prolapse had no abnormal auscultatory signs. Of the 93 babies without evidence of mitral prolapse, 91 had normal echocardiograms and auscultatory features; one was noted to have a murmur consistent with a ventricular septal defect, and another had an eccentric aortic valve on the echocardiogram which was suggestive of a bicuspid aortic valve. Serial studies on our group of subjects will yield useful information regarding the natural history of mitral valve prolapse."}
{"id": "PMID:474380", "title": "Electrophysiological effects of disopyramide phosphate during experimental myocardial ischemia.", "content": "In order to correlate the antiarrhythmic and electrophysiological effects of disopyramide phosphate during acute myocardial ischemia, we performed experiments in 17 mongrel dogs. Refractory periods obtained by the extrastimulus method and conduction times recorded from local electrograms were determined in potentially ischemic and nonischemic areas prior to, after left anterior descending coronary occlusion, and following intravenous administration of disopyramide phosphate 3 mg./Kg. Control refractory periods were similar in both nonischemic and ischemic areas. Following coronary ligation, a disparity of refractoriness of 28 msec. was induced between these two areas. After disopyramide administration, this disparity was reduced from 28 msec. to 5 msec. (p less than 0.001) after 5 to 15 minutes, and to 15 msec. (p less than 0.01) after 15 to 30 minutes. Coronary ligation prolonged conduction times by 8 msec. (p less than 0.005) in ischemic areas and disopyramide further prolonged conduction in these areas by an additional 9 msec. (p less than 0.001). A minimal and transient prolongation of conduction was present in nonischemic areas. We conclude that the differential effects exerted by disopyramide phosphate in ischemic areas may explain its suppressant action of arrhythmias of ventricular origin.", "contents": "Electrophysiological effects of disopyramide phosphate during experimental myocardial ischemia. In order to correlate the antiarrhythmic and electrophysiological effects of disopyramide phosphate during acute myocardial ischemia, we performed experiments in 17 mongrel dogs. Refractory periods obtained by the extrastimulus method and conduction times recorded from local electrograms were determined in potentially ischemic and nonischemic areas prior to, after left anterior descending coronary occlusion, and following intravenous administration of disopyramide phosphate 3 mg./Kg. Control refractory periods were similar in both nonischemic and ischemic areas. Following coronary ligation, a disparity of refractoriness of 28 msec. was induced between these two areas. After disopyramide administration, this disparity was reduced from 28 msec. to 5 msec. (p less than 0.001) after 5 to 15 minutes, and to 15 msec. (p less than 0.01) after 15 to 30 minutes. Coronary ligation prolonged conduction times by 8 msec. (p less than 0.005) in ischemic areas and disopyramide further prolonged conduction in these areas by an additional 9 msec. (p less than 0.001). A minimal and transient prolongation of conduction was present in nonischemic areas. We conclude that the differential effects exerted by disopyramide phosphate in ischemic areas may explain its suppressant action of arrhythmias of ventricular origin."}
{"id": "PMID:474382", "title": "Spontaneous resumption of sinus rhythm in an elderly patient after 13 years of permanent atrial fibrillation.", "content": "One female patient--with slight pure mitral stenosis, mild hypertension and ischemic cardiomyopathy and disabling pulmonary emphysema--developed at 54 years of age permanent atrial fibrillation, had a gratuitous mitral commissurotomy four months later, sustained chronic fibrillation for 13 years, then spontaneously resumed sinus node command at age 67 without any discernible reason. Sinus rhythm was being maintained at follow-up nine months later. Her cardiac status of fair compensation under modest digitoxin and diuretic therapy has neither improved nor worsened with the return of atrial systole. The duration, in this observation, of permanent auricular fibrillation before spontaneous return of sinus rhythm, is one of the longest ever published, exceeded, to the best of my knowledge, only by one case of Lewis and by another one of Reeve and associates. Such an exceptional event points out a fascinating enigma: how can major longstanding atrial dysrhythmias (fibrillation, flutter), whose causes and pathogenesis seem at least partly elucidated, spontaneously disappeer in atria so badly diseased? I think we must humbly confess that no satisfactory explanation is at present available for this disconcerting phenomenon.", "contents": "Spontaneous resumption of sinus rhythm in an elderly patient after 13 years of permanent atrial fibrillation. One female patient--with slight pure mitral stenosis, mild hypertension and ischemic cardiomyopathy and disabling pulmonary emphysema--developed at 54 years of age permanent atrial fibrillation, had a gratuitous mitral commissurotomy four months later, sustained chronic fibrillation for 13 years, then spontaneously resumed sinus node command at age 67 without any discernible reason. Sinus rhythm was being maintained at follow-up nine months later. Her cardiac status of fair compensation under modest digitoxin and diuretic therapy has neither improved nor worsened with the return of atrial systole. The duration, in this observation, of permanent auricular fibrillation before spontaneous return of sinus rhythm, is one of the longest ever published, exceeded, to the best of my knowledge, only by one case of Lewis and by another one of Reeve and associates. Such an exceptional event points out a fascinating enigma: how can major longstanding atrial dysrhythmias (fibrillation, flutter), whose causes and pathogenesis seem at least partly elucidated, spontaneously disappeer in atria so badly diseased? I think we must humbly confess that no satisfactory explanation is at present available for this disconcerting phenomenon."}
{"id": "PMID:474383", "title": "Traumatic pulmonary artery--left atrial fistula: an unusual case of cyanosis in an adult.", "content": "Eighteen months after sustaining a stab wound to the left upper chest, a 59-year-old man presented with cyanosis and extertional dyspnea. Arterial desaturation due to a central 22 per cent right-to-left shunt was present. A selective pulmonary arteriogram demonstrated a fistula between the main pulmonary artery and the left atrium. At operation the fistula was closed. A laceration of the pulmonic valve and healed pericarditis were present. Marked symptomatic improvement followed the operation, but a murmur of pulmonic valvular regurgitation persisted. The fistula and laceration of the pulmonic valve were probably traumatic in origin.", "contents": "Traumatic pulmonary artery--left atrial fistula: an unusual case of cyanosis in an adult. Eighteen months after sustaining a stab wound to the left upper chest, a 59-year-old man presented with cyanosis and extertional dyspnea. Arterial desaturation due to a central 22 per cent right-to-left shunt was present. A selective pulmonary arteriogram demonstrated a fistula between the main pulmonary artery and the left atrium. At operation the fistula was closed. A laceration of the pulmonic valve and healed pericarditis were present. Marked symptomatic improvement followed the operation, but a murmur of pulmonic valvular regurgitation persisted. The fistula and laceration of the pulmonic valve were probably traumatic in origin."}
{"id": "PMID:474410", "title": "Developing and implementing an industrial hygiene and safety program in industry.", "content": "The development and implementation of a comprehensive industrial hygiene and safety program is a complex activity for which there does not exist a uniform code or set of guidelines. Although the ultimate goal of such a program is to provide for the welfare of the employee through the use of engineering control and other preventive measures, there is great variation among business establishments on methods necessary to attain this result. Factors which contribute to this variation usually are: type of industry, management philosophy and size of the business establishment. This paper addresses many key elements integral to this activity such as justification and philosophy, organizational reporting relationships, interfunctional communication, technical and personnel requirements and program performance indicators.", "contents": "Developing and implementing an industrial hygiene and safety program in industry. The development and implementation of a comprehensive industrial hygiene and safety program is a complex activity for which there does not exist a uniform code or set of guidelines. Although the ultimate goal of such a program is to provide for the welfare of the employee through the use of engineering control and other preventive measures, there is great variation among business establishments on methods necessary to attain this result. Factors which contribute to this variation usually are: type of industry, management philosophy and size of the business establishment. This paper addresses many key elements integral to this activity such as justification and philosophy, organizational reporting relationships, interfunctional communication, technical and personnel requirements and program performance indicators."}
{"id": "PMID:474413", "title": "Quantitative fit-test method for powered air-purifying respirators.", "content": "The fit-test method for powered air-purifying respirators described here allowed quantitative determination of the leakage of PAPRs that offered PFs ranging from 50 to greater than 10,000. Five types of PAPRs were tested on 10 subjects selected from a test panel representing a wide range of facial sizes. PAPRs tested provided all subjects with PFs of 1000 or greater when operated with the blowers on, while PFs observed with the blower off, for two tight-fitting facepieces, ranged between 50 and 100. The exercises used in these test procedures are believed to be typical of movements made by persons wearing respiratory protective equipment and performing light work. Studies of PFs obtained by workers wearing respiratory protective equipment in industry would be necessary to establish how well the developed laboratory test methods predict the level of protection offered to workers wearing the equipment.", "contents": "Quantitative fit-test method for powered air-purifying respirators. The fit-test method for powered air-purifying respirators described here allowed quantitative determination of the leakage of PAPRs that offered PFs ranging from 50 to greater than 10,000. Five types of PAPRs were tested on 10 subjects selected from a test panel representing a wide range of facial sizes. PAPRs tested provided all subjects with PFs of 1000 or greater when operated with the blowers on, while PFs observed with the blower off, for two tight-fitting facepieces, ranged between 50 and 100. The exercises used in these test procedures are believed to be typical of movements made by persons wearing respiratory protective equipment and performing light work. Studies of PFs obtained by workers wearing respiratory protective equipment in industry would be necessary to establish how well the developed laboratory test methods predict the level of protection offered to workers wearing the equipment."}
{"id": "PMID:474414", "title": "Electromagnetic radiation emitted from video computer terminals.", "content": "There has been a developing concern about potential health hazards attributable to electromagnetic radiation from video computer terminals. Since users of these devices typically spend many hours daily in close proximity, they would be subject to chronic long-term exposures to any emitted radiation. Radiation was detected at frequencies ranging from 1.5 kHz to 1,420 MHz in the RF portion of the EM spectrum and from 350 nm to 600 nm in the ultraviolet and visible region. However, the levels were far less than the most restrictive permissible exposure levels established by any agency or government of any nation. There is no experimental or epidemiological evidence presently available to indicate that these levels of RF radiation have any deleterious effects on the human biological system.", "contents": "Electromagnetic radiation emitted from video computer terminals. There has been a developing concern about potential health hazards attributable to electromagnetic radiation from video computer terminals. Since users of these devices typically spend many hours daily in close proximity, they would be subject to chronic long-term exposures to any emitted radiation. Radiation was detected at frequencies ranging from 1.5 kHz to 1,420 MHz in the RF portion of the EM spectrum and from 350 nm to 600 nm in the ultraviolet and visible region. However, the levels were far less than the most restrictive permissible exposure levels established by any agency or government of any nation. There is no experimental or epidemiological evidence presently available to indicate that these levels of RF radiation have any deleterious effects on the human biological system."}
{"id": "PMID:474415", "title": "Respiratory retention and acute toxicity of furan.", "content": "The respiratory tract retention of furan has been studied in dogs as part of a broad investigation of compounds found in the vapor phase of cigarette smoke. The LD50 of furan has also been determined in mice and rats and the LC50 in mice. Respiratory uptake of furan was between 90 and 95%, varying inversely with the ventilatory rate. Retention was not affected by tidal volume changes but was directly related to concentration inhaled. Intraperitoneal LD50 values were 5.2 mg/kg for rats and 7.0 mg/kg for mice. The LC50 for mice was 0.12 microgram/mL. These results indicate the high toxicity of furan and that it is readily absorbed by the inhalation route.", "contents": "Respiratory retention and acute toxicity of furan. The respiratory tract retention of furan has been studied in dogs as part of a broad investigation of compounds found in the vapor phase of cigarette smoke. The LD50 of furan has also been determined in mice and rats and the LC50 in mice. Respiratory uptake of furan was between 90 and 95%, varying inversely with the ventilatory rate. Retention was not affected by tidal volume changes but was directly related to concentration inhaled. Intraperitoneal LD50 values were 5.2 mg/kg for rats and 7.0 mg/kg for mice. The LC50 for mice was 0.12 microgram/mL. These results indicate the high toxicity of furan and that it is readily absorbed by the inhalation route."}
{"id": "PMID:474416", "title": "Service station attendants' exposure to benzene and gasoline vapors.", "content": "Service station attendants' exposure to benzene, based on 85 TWA results at 7 stations, were well below 1 ppm except one exposure of 2.08 ppm. Short term exposures were 1.21 ppm or less over 15 minutes. Attendants' TWA exposures to total gasoline vapor were 114 ppm or less, with measured 15 minute exposures no higher than 100 ppm during actual filling operations. One station had vapor recovery nozzles; exposures here were below the detectable level (0.01 ppm benzene) on 10% more days than the next lowest station. Still, the magnitude of overall exposures and the degree of reduction indicate that vapor recovery is not needed to control exposures. Some attendants had consistently higher exposures than others. This is felt to be due to work practices, such as standing close to the fill opening, plus local wind conditions around the car as it is filled with gasoline.", "contents": "Service station attendants' exposure to benzene and gasoline vapors. Service station attendants' exposure to benzene, based on 85 TWA results at 7 stations, were well below 1 ppm except one exposure of 2.08 ppm. Short term exposures were 1.21 ppm or less over 15 minutes. Attendants' TWA exposures to total gasoline vapor were 114 ppm or less, with measured 15 minute exposures no higher than 100 ppm during actual filling operations. One station had vapor recovery nozzles; exposures here were below the detectable level (0.01 ppm benzene) on 10% more days than the next lowest station. Still, the magnitude of overall exposures and the degree of reduction indicate that vapor recovery is not needed to control exposures. Some attendants had consistently higher exposures than others. This is felt to be due to work practices, such as standing close to the fill opening, plus local wind conditions around the car as it is filled with gasoline."}
{"id": "PMID:474417", "title": "Respirable particulates generated by pressurized consumer products. I. Experimental method and general characteristics.", "content": "Many products and materials are available in the widely used pressurized package or \"aerosol\" form. A simulated breathing zone model approach has been developed and used to characterize the particles to which a user may be exposed. Some products characterized under simulated \"worst reasonable\" conditions produced concentrations of particulates smaller than 6 micrometer aerodynamic diameter exceeding 50 mg/m3.", "contents": "Respirable particulates generated by pressurized consumer products. I. Experimental method and general characteristics. Many products and materials are available in the widely used pressurized package or \"aerosol\" form. A simulated breathing zone model approach has been developed and used to characterize the particles to which a user may be exposed. Some products characterized under simulated \"worst reasonable\" conditions produced concentrations of particulates smaller than 6 micrometer aerodynamic diameter exceeding 50 mg/m3."}
{"id": "PMID:474418", "title": "Respirable particulates generated by pressurized consumer products. II. Influence of experimental conditions.", "content": "The many products available in pressurized packages produce appreciable concentrations of particulates that may be deposited in the deep lung of human users. We have reported the size and concentration characteristics of these aerosols under carefully standardized conditions in a previous paper. These standard conditions have been varied to determine the influence of product discharge conditions on observed aerosol characteristics. The size characteristics are relatively insensitive to a wide range of changes in the discharge conditions; the only significant effect observed followed changing the target of the spray from a steel plate to a wig. The aerosol concentrations were changed by several experimental factors, but the relative changes were less than the estimated relative range of human exposure.", "contents": "Respirable particulates generated by pressurized consumer products. II. Influence of experimental conditions. The many products available in pressurized packages produce appreciable concentrations of particulates that may be deposited in the deep lung of human users. We have reported the size and concentration characteristics of these aerosols under carefully standardized conditions in a previous paper. These standard conditions have been varied to determine the influence of product discharge conditions on observed aerosol characteristics. The size characteristics are relatively insensitive to a wide range of changes in the discharge conditions; the only significant effect observed followed changing the target of the spray from a steel plate to a wig. The aerosol concentrations were changed by several experimental factors, but the relative changes were less than the estimated relative range of human exposure."}
{"id": "PMID:474422", "title": "Coexistence of ventricular parasystole and ventricular couplets: mechanism and clinical significance.", "content": "In 78 consecutive patients with uniform ventricular ectopic complexes and without heart disease, ventricular couplets were present significantly more often when the coupling interval of ventricular ectopic complexes was variable than when it was fixed (P less than 0.04). In 69 consecutive patients with couplets, the prevalence of a variable coupling interval was significantly greater than that of (55 versus 14 cases; P less than 0.001). Among 55 patients with a variable coupling interval, ventricular parasystole was probable in 38 and possible in 17 patients. These results suggest that the association between ventricular couplets and parasystole is not coincidental. Of several possible mechanisms responsible for this association reentry within the parasystolic focus or its vicinity is the most probable. This may explain the observation that the couplets are seldom followed by consecutive ventricular ectopic complexes or ventricular tachycardia. If this hypothesis is correct, the clinical significance of ventricular couplets in the presence of ventricular parasystole may be similar to that of single reentrant ventricular ectopic complexes.", "contents": "Coexistence of ventricular parasystole and ventricular couplets: mechanism and clinical significance. In 78 consecutive patients with uniform ventricular ectopic complexes and without heart disease, ventricular couplets were present significantly more often when the coupling interval of ventricular ectopic complexes was variable than when it was fixed (P less than 0.04). In 69 consecutive patients with couplets, the prevalence of a variable coupling interval was significantly greater than that of (55 versus 14 cases; P less than 0.001). Among 55 patients with a variable coupling interval, ventricular parasystole was probable in 38 and possible in 17 patients. These results suggest that the association between ventricular couplets and parasystole is not coincidental. Of several possible mechanisms responsible for this association reentry within the parasystolic focus or its vicinity is the most probable. This may explain the observation that the couplets are seldom followed by consecutive ventricular ectopic complexes or ventricular tachycardia. If this hypothesis is correct, the clinical significance of ventricular couplets in the presence of ventricular parasystole may be similar to that of single reentrant ventricular ectopic complexes."}
{"id": "PMID:474423", "title": "Interatrial conduction (activation) times.", "content": "Left atrial electrograms were recorded simultaneously from sites located in the coronary sinus, right pulmonary artery and esophagus in order to determine and correlate the interatrial conduction times obtained from these locations. Ten patients with normal-sized atria and 16 patients with isolated left atrial enlargement were studied. A high correlation existed between the P wave duration and the interatrial conduction times obtained from the coronary sinus and pulmonary artery; good correlations were also found for the interatrial conduction times recorded from the pulmonary artery and esophagus correlated well with those recorded from the distal coronary sinus (the generally accepted standard for the indirect interatrial conduction time measurements). In addition to providing information on the mechanism of atrial arrhythmias, the electrograms obtained from the various indirect left atrial recording sites provide satisfactory quantification of interatrial conduction.", "contents": "Interatrial conduction (activation) times. Left atrial electrograms were recorded simultaneously from sites located in the coronary sinus, right pulmonary artery and esophagus in order to determine and correlate the interatrial conduction times obtained from these locations. Ten patients with normal-sized atria and 16 patients with isolated left atrial enlargement were studied. A high correlation existed between the P wave duration and the interatrial conduction times obtained from the coronary sinus and pulmonary artery; good correlations were also found for the interatrial conduction times recorded from the pulmonary artery and esophagus correlated well with those recorded from the distal coronary sinus (the generally accepted standard for the indirect interatrial conduction time measurements). In addition to providing information on the mechanism of atrial arrhythmias, the electrograms obtained from the various indirect left atrial recording sites provide satisfactory quantification of interatrial conduction."}
{"id": "PMID:474426", "title": "Assessment of early ventricular systole by first pass radionuclide angiography: useful method for detection of left ventricular dysfunction at rest in patients with coronary artery disease.", "content": "To identify abnormal left ventricular function without exercise stress in patients with coronary artery disease first-pass radionuclide angiograms were analyzed in 32 normal subjects (Group I); 31 patients with coronary disease and normal contrast ventriculograms (Group II); and 17 patients with coronary disease and depressed left ventricular function (Group III). Total ejection fraction (EF) was computed with standard angiographic methods and from each time-activity curve. During the first third of systole, ejection fraction was determined manually by averaging three to five beats and the value compared with that obtained with contrast ventriculography: (Formula: see text). Both total radionuclide ejection fraction (r = 0.95) and first-third ejection fraction (r = 0.91) correlated well with angiography. Intraobserver and interobserver variation was small, averaging 0.02 +/- 0.02 (range 0 to 0.05). The radionuclide first-third ejection fraction was 0.25 or greater in normal subjects and less than 0.25 in 29 of 31 patients (94 percent) in Group II and in all patients in Group III. It is concluded that the first-third ejection fraction obtained with first pass angiography identifies subtle abnormalities of left ventricular function at rest in more than 90 percent of patients with coronary disease that may not be recognized by total ejection fraction alone.", "contents": "Assessment of early ventricular systole by first pass radionuclide angiography: useful method for detection of left ventricular dysfunction at rest in patients with coronary artery disease. To identify abnormal left ventricular function without exercise stress in patients with coronary artery disease first-pass radionuclide angiograms were analyzed in 32 normal subjects (Group I); 31 patients with coronary disease and normal contrast ventriculograms (Group II); and 17 patients with coronary disease and depressed left ventricular function (Group III). Total ejection fraction (EF) was computed with standard angiographic methods and from each time-activity curve. During the first third of systole, ejection fraction was determined manually by averaging three to five beats and the value compared with that obtained with contrast ventriculography: (Formula: see text). Both total radionuclide ejection fraction (r = 0.95) and first-third ejection fraction (r = 0.91) correlated well with angiography. Intraobserver and interobserver variation was small, averaging 0.02 +/- 0.02 (range 0 to 0.05). The radionuclide first-third ejection fraction was 0.25 or greater in normal subjects and less than 0.25 in 29 of 31 patients (94 percent) in Group II and in all patients in Group III. It is concluded that the first-third ejection fraction obtained with first pass angiography identifies subtle abnormalities of left ventricular function at rest in more than 90 percent of patients with coronary disease that may not be recognized by total ejection fraction alone."}
{"id": "PMID:474429", "title": "Echocardiographic assessment of the adequacy of pulmonary arterial banding.", "content": "Thirty-one echocardiograms of 21 patients who had pumonary arterial banding were analyzed to assess the aequacy of surgery. In 5 patients the echocardiograms were obtained before and after banding and in 16 patients only after surgery. Right and left ventricular systolic time intervals were measured echographically. The ratios of the right ventricular preejection period to right ventricular ejection time (RPEP/RVET) were correlated with both diastolic (r = 0.94) and systolic (r = 0.86) pulmonary arterial pressures distal to the band. The analysis of right ventriclar systolic time intervals, especially the RPEP/RVET ratio, clearly differentiated patients with an adequate band (distal pulmonary arterial diastolic pressure less than 15 mm Hg) from patients with an inadequate band (distal pulmonary arterial diastolic pressure equal to or greater than 30 mm Hg). The results indicate that echocardiography is a useful noninvasive tool in evaluating the adequacy of the pulmonary arterial band and facilitates the follow-up of patients after banding.", "contents": "Echocardiographic assessment of the adequacy of pulmonary arterial banding. Thirty-one echocardiograms of 21 patients who had pumonary arterial banding were analyzed to assess the aequacy of surgery. In 5 patients the echocardiograms were obtained before and after banding and in 16 patients only after surgery. Right and left ventricular systolic time intervals were measured echographically. The ratios of the right ventricular preejection period to right ventricular ejection time (RPEP/RVET) were correlated with both diastolic (r = 0.94) and systolic (r = 0.86) pulmonary arterial pressures distal to the band. The analysis of right ventriclar systolic time intervals, especially the RPEP/RVET ratio, clearly differentiated patients with an adequate band (distal pulmonary arterial diastolic pressure less than 15 mm Hg) from patients with an inadequate band (distal pulmonary arterial diastolic pressure equal to or greater than 30 mm Hg). The results indicate that echocardiography is a useful noninvasive tool in evaluating the adequacy of the pulmonary arterial band and facilitates the follow-up of patients after banding."}
{"id": "PMID:474430", "title": "Incessant atrial tachycardia in childhood: association with rate-dependent conduction in an accessory atrioventricular pathway.", "content": "A detailed electrophysiologic study in a 7 year old boy with incessant atrial tachycardia revealed the presence of an atrioventricular pathway conducting only in the ventriculoatrial direction. The results indicate a reentrant mechanism with the retrograde limb of the circuit entering the atrium in the region of the proximal to mid coronary sinus. The accessory pathway exhibited relatively long conduction times and conduction properties suggesting decremental conduction. Thus, an electrophysiologically atypical anomalous conduction pathway may be responsible for the occurrence of incessant atrial tachycardial in some children.", "contents": "Incessant atrial tachycardia in childhood: association with rate-dependent conduction in an accessory atrioventricular pathway. A detailed electrophysiologic study in a 7 year old boy with incessant atrial tachycardia revealed the presence of an atrioventricular pathway conducting only in the ventriculoatrial direction. The results indicate a reentrant mechanism with the retrograde limb of the circuit entering the atrium in the region of the proximal to mid coronary sinus. The accessory pathway exhibited relatively long conduction times and conduction properties suggesting decremental conduction. Thus, an electrophysiologically atypical anomalous conduction pathway may be responsible for the occurrence of incessant atrial tachycardial in some children."}
{"id": "PMID:474431", "title": "Systolic hypertension: hemodynamic mechanism and choice of antihypertensive treatment.", "content": "Arterial compliance and indexes of ventricular ejection were measured in 27 men with systolic hypertension. The patients were separated into two age groups, younger or older than age 35 years, and matched with normotensive control subjects. Arterial compliance was estimated from analysis of the monoexponential blood pressure-time curve during diastole, according to a simple viscoelastic model. In the younger patients, arterial compliance and stroke volume were within normal ranges. Rapid ejection time was significantly reduced (P less than 0.001), indicating an increased venlocity in the first part of ventricular ejection. Systolic pressure decreased significantly after administration of propranolol, which also caused prolongation of rapid ejection time. In the older patients, indexes of ventricular ejection were within normal limits. arterial compliance was significantly reduced (P less than 0.01) and was negatively correlated with the level of systolic pressure (P less than 0.001). Systolic pressure decreased significantly after administration of sodium nitroprusside, which caused an increase in arterial compliance. These findings provide evidence that: (1) the hemodynamic mechanisms of systolic hypertension differ in younger and older patients, and (2) these hemodynamic differences should be taken into account when choosing drugs to decrease systolic pressure.", "contents": "Systolic hypertension: hemodynamic mechanism and choice of antihypertensive treatment. Arterial compliance and indexes of ventricular ejection were measured in 27 men with systolic hypertension. The patients were separated into two age groups, younger or older than age 35 years, and matched with normotensive control subjects. Arterial compliance was estimated from analysis of the monoexponential blood pressure-time curve during diastole, according to a simple viscoelastic model. In the younger patients, arterial compliance and stroke volume were within normal ranges. Rapid ejection time was significantly reduced (P less than 0.001), indicating an increased venlocity in the first part of ventricular ejection. Systolic pressure decreased significantly after administration of propranolol, which also caused prolongation of rapid ejection time. In the older patients, indexes of ventricular ejection were within normal limits. arterial compliance was significantly reduced (P less than 0.01) and was negatively correlated with the level of systolic pressure (P less than 0.001). Systolic pressure decreased significantly after administration of sodium nitroprusside, which caused an increase in arterial compliance. These findings provide evidence that: (1) the hemodynamic mechanisms of systolic hypertension differ in younger and older patients, and (2) these hemodynamic differences should be taken into account when choosing drugs to decrease systolic pressure."}
{"id": "PMID:474432", "title": "Suppression of ventricular arrhythmias with intravenous disopyramide and lidocaine: efficacy comparison in a randomized trial.", "content": "Twenty-six patients with clinically significant ventricular arrhythmias were randomly assigned to treatment with either intravenous disopyramide or lidocaine; crossover to the other agent was permitted in nine cases of primary drug failure. In addition, disopyramide was administered nonrandomly to seven patients with ventricular arrhythmias not controlled by lidocaine in standard doses. Arrhythmia control (greater than 50 percent reduction of premature ventricular complexes) was achieved in all 22 trials with disopyramide and in 9 of 13 trails with lidocaine in the random study, whereas clinical efficacy (arrhythmia control with absence of side effects) occurred respectively in 15 of 22, and 8 of 13 trials. In all 11 patients (7 nonrandom, 4 random) whose arrhythmia was not controlled with lidocaine the arrhythmia was controlled with disopyramide. Thus, the clinical efficacy of intravenous disopyramide ran parallel to that of lidocaine in patients with ventricular arrhythmias. Furthermore, intravenous disopyramide was an effective alternative agent for patients with arrhythmia not controlled by lidocaine.", "contents": "Suppression of ventricular arrhythmias with intravenous disopyramide and lidocaine: efficacy comparison in a randomized trial. Twenty-six patients with clinically significant ventricular arrhythmias were randomly assigned to treatment with either intravenous disopyramide or lidocaine; crossover to the other agent was permitted in nine cases of primary drug failure. In addition, disopyramide was administered nonrandomly to seven patients with ventricular arrhythmias not controlled by lidocaine in standard doses. Arrhythmia control (greater than 50 percent reduction of premature ventricular complexes) was achieved in all 22 trials with disopyramide and in 9 of 13 trails with lidocaine in the random study, whereas clinical efficacy (arrhythmia control with absence of side effects) occurred respectively in 15 of 22, and 8 of 13 trials. In all 11 patients (7 nonrandom, 4 random) whose arrhythmia was not controlled with lidocaine the arrhythmia was controlled with disopyramide. Thus, the clinical efficacy of intravenous disopyramide ran parallel to that of lidocaine in patients with ventricular arrhythmias. Furthermore, intravenous disopyramide was an effective alternative agent for patients with arrhythmia not controlled by lidocaine."}
{"id": "PMID:474433", "title": "Clinical electrophysiologic effects of encainide, a newly developed antiarrhythmic agent.", "content": "Encainide is a newly developed antiarrhythmic agent. With the use of intracardiac electrophysiologic techniques, its effects on the cardiac conduction system were examined in 10 patients with coronary artery disease. Five patients received 0.6 and five received 0.9 mg/kg body weight of encainide intravenously over 15 minutes. Plasma concentration, heart rate, blood pressure and conduction intervals (A-H, H-V, QRS and Q-T) were measured before, during and after encainide infusion. In addition, sinus nodal recovery time, Wenckebach cycle length, and atrial, atrioventricular (A-V) nodal and right ventricular refractory periods were measured before and after encainide infusion. The average peak plasma concentration was 0.49 +/- 0.35 microgram/ml (mean +/- standard error of the mean). Encainide significantly prologned H-V and QRS intervals in all patients by an average of 31 +/- 7 and 18 +/- 9 percent (standard deviation) (P less than 0.001), respectively. A minimal increase in the Q-T interval was also observed after encainide infusion (2 +/- 9 percent, P less than 0.01), but no significant changes were noted in heart rate, blood pressure, A-H interval, corrected sinus noal recovery time, Wenckebach cycle length or refractory periods of the atrium, A-V node or right ventricle. It is concluded that encainide significantly prolongs conduction in the His-Purkinje system without affecting conduction or refractoriness of other parts of the cardiac conduction system in man.", "contents": "Clinical electrophysiologic effects of encainide, a newly developed antiarrhythmic agent. Encainide is a newly developed antiarrhythmic agent. With the use of intracardiac electrophysiologic techniques, its effects on the cardiac conduction system were examined in 10 patients with coronary artery disease. Five patients received 0.6 and five received 0.9 mg/kg body weight of encainide intravenously over 15 minutes. Plasma concentration, heart rate, blood pressure and conduction intervals (A-H, H-V, QRS and Q-T) were measured before, during and after encainide infusion. In addition, sinus nodal recovery time, Wenckebach cycle length, and atrial, atrioventricular (A-V) nodal and right ventricular refractory periods were measured before and after encainide infusion. The average peak plasma concentration was 0.49 +/- 0.35 microgram/ml (mean +/- standard error of the mean). Encainide significantly prologned H-V and QRS intervals in all patients by an average of 31 +/- 7 and 18 +/- 9 percent (standard deviation) (P less than 0.001), respectively. A minimal increase in the Q-T interval was also observed after encainide infusion (2 +/- 9 percent, P less than 0.01), but no significant changes were noted in heart rate, blood pressure, A-H interval, corrected sinus noal recovery time, Wenckebach cycle length or refractory periods of the atrium, A-V node or right ventricle. It is concluded that encainide significantly prolongs conduction in the His-Purkinje system without affecting conduction or refractoriness of other parts of the cardiac conduction system in man."}
{"id": "PMID:474435", "title": "Prenatal closure of the foramen ovale in complete transposition of great vessels. Observations in a case with intact ventricular septum and normally developed left heart chambers.", "content": "A rare case is reported of premature closure of the foramen ovale in d-transposition of the great vessels with intact ventricular septum and normal development of the left side of the heart. Asphyxia neoatorum and cardiac failure were the manifestations in the early neonatal period. Balloon atrial septostomy during cardiac catheterization would be unsuccessful in infants with this condition, and closed atrial septectomy (Blalock-Hanlon procedure) would be necessary for survival.", "contents": "Prenatal closure of the foramen ovale in complete transposition of great vessels. Observations in a case with intact ventricular septum and normally developed left heart chambers. A rare case is reported of premature closure of the foramen ovale in d-transposition of the great vessels with intact ventricular septum and normal development of the left side of the heart. Asphyxia neoatorum and cardiac failure were the manifestations in the early neonatal period. Balloon atrial septostomy during cardiac catheterization would be unsuccessful in infants with this condition, and closed atrial septectomy (Blalock-Hanlon procedure) would be necessary for survival."}
{"id": "PMID:474443", "title": "Subdivision of mouse vibrissae on an embryological basis, with descriptions of variations in the number and arrangement of sinus hairs and cortical barrels in BALB/c (nu/+; nude, nu/nu) and hairless (hr/hr) strains.", "content": "Development of vibrissae was studied in dd/y mouse embryos by scanning electron microscopy. Arrangement of vibrissae and cortical barrels were also studied by light microscopy in adult dd/y, BALB/c(nu/+), nude (BALB/c, nu/nu) and hairless (hr/hr) mice to find genetic or epigenetic variations. Rudiments of vibrissae first appear on Day 12 of pregnancy as longitudinal ridges on the developing muzzle, and each hair rudiment is represented by a dome on the ridges. The dorsal two rows (A and B; Woolsey and Van der Loos, '70) of mystacial vibrissae are on the lateral nasal prominence, while the ventral three (C, D and E) are on the maxillary prominence. Smaller hairs of mystacial vibrissae appear at the labial part of the maxillary prominenceon Day 13. The rudiments of rhinal hairs also appear at this stage on the part of the muzzle derived from the medial nasal prominence. Thus the so-called mystacial vibrissae should be subdivided into three (or 4, including the rhinal) groups on an embryological basis. They are the lateral nasal, the maxillary and the labial. A supernumerary sinus hair and a corresponding barrel was observed between D and C rows uni-or bilaterally in one third of individuals of BALB/c, nude and hairless mice. It is suggested that supernumerary hairs tend to occur between the groups of hairs as defined above. In nude and hairless mice small barrels representing labial hairs are diminished in number. The number of hair follicles, however, is normal.", "contents": "Subdivision of mouse vibrissae on an embryological basis, with descriptions of variations in the number and arrangement of sinus hairs and cortical barrels in BALB/c (nu/+; nude, nu/nu) and hairless (hr/hr) strains. Development of vibrissae was studied in dd/y mouse embryos by scanning electron microscopy. Arrangement of vibrissae and cortical barrels were also studied by light microscopy in adult dd/y, BALB/c(nu/+), nude (BALB/c, nu/nu) and hairless (hr/hr) mice to find genetic or epigenetic variations. Rudiments of vibrissae first appear on Day 12 of pregnancy as longitudinal ridges on the developing muzzle, and each hair rudiment is represented by a dome on the ridges. The dorsal two rows (A and B; Woolsey and Van der Loos, '70) of mystacial vibrissae are on the lateral nasal prominence, while the ventral three (C, D and E) are on the maxillary prominence. Smaller hairs of mystacial vibrissae appear at the labial part of the maxillary prominenceon Day 13. The rudiments of rhinal hairs also appear at this stage on the part of the muzzle derived from the medial nasal prominence. Thus the so-called mystacial vibrissae should be subdivided into three (or 4, including the rhinal) groups on an embryological basis. They are the lateral nasal, the maxillary and the labial. A supernumerary sinus hair and a corresponding barrel was observed between D and C rows uni-or bilaterally in one third of individuals of BALB/c, nude and hairless mice. It is suggested that supernumerary hairs tend to occur between the groups of hairs as defined above. In nude and hairless mice small barrels representing labial hairs are diminished in number. The number of hair follicles, however, is normal."}
{"id": "PMID:474444", "title": "Development of lamellar bodies and subsurface cisterns in pyramidal cells and neuroblasts of hamster cerebral cortex.", "content": "In pyramidal cells of hamster frontal cortex lamellar bodies and subsurface cisterns sequentially occurred during development from newborn to three months of age. Neither of these two specializations of the rough endoplasmic reticulum was seen in neurons of the newborn. The first specialization that we observed was the subsurface cistern, which appeared at five days and showed a significant increase both in frequency and in length throughout development. The lamellar body was first seen at ten days of age. This specialization showed a peaking of frequency, length, and number of cisterns per body at 15 days, which subsequently decreased gradually to 3-month-old levels. The occurrence of lamellar body-subsurface cistern complexes increased with age. We suggest that the lamellar body may be an ER specialization that is involved in an increased and/or stage-specific protein synthesis in the young prior to the final maturation of the usual neuronal protein synthetic organelles, and that in the 3-month-old neurons, the lamellar body may be involved in modifying, storing or transporting metabolites received from the neuropil components via the subsurface cisterns. The subsurface cistern, on the other hand, by virtue of its location subjacent to the neuronal plasma membrane and of its increased frequency from birth to maturation, may be involved in the exchange of metabolites and nonsynaptic forms of communication at all ages.", "contents": "Development of lamellar bodies and subsurface cisterns in pyramidal cells and neuroblasts of hamster cerebral cortex. In pyramidal cells of hamster frontal cortex lamellar bodies and subsurface cisterns sequentially occurred during development from newborn to three months of age. Neither of these two specializations of the rough endoplasmic reticulum was seen in neurons of the newborn. The first specialization that we observed was the subsurface cistern, which appeared at five days and showed a significant increase both in frequency and in length throughout development. The lamellar body was first seen at ten days of age. This specialization showed a peaking of frequency, length, and number of cisterns per body at 15 days, which subsequently decreased gradually to 3-month-old levels. The occurrence of lamellar body-subsurface cistern complexes increased with age. We suggest that the lamellar body may be an ER specialization that is involved in an increased and/or stage-specific protein synthesis in the young prior to the final maturation of the usual neuronal protein synthetic organelles, and that in the 3-month-old neurons, the lamellar body may be involved in modifying, storing or transporting metabolites received from the neuropil components via the subsurface cisterns. The subsurface cistern, on the other hand, by virtue of its location subjacent to the neuronal plasma membrane and of its increased frequency from birth to maturation, may be involved in the exchange of metabolites and nonsynaptic forms of communication at all ages."}
{"id": "PMID:474445", "title": "Development of the renal corpuscle during metamorphosis in the lamprey.", "content": "The renal corpuscle of the adult lamprey, Petromyzon marinus L., is formed during the programmed period of metamorphosis. Development is initiated early in this metamorphic period and is marked by the synchronous formation and growth of rudimentary nephron units (RNU) from longitudinal cord of nephrogenictissue extending from the posterior tip of the degenerating larval kidney to the cloaca and connected to the peritoneal epithelium. Detachment of the RNU from the peritoneum involves autolysis and cell death and is accompanied by their branching into five or six hexagonally-arranged nephrons which radiate from the original point of attachment. Differentiation of the epithelial cells at the proximal ends of the nephrons is preceded by the widening of lateral intercellular spaces, the formation of tubular lumina (primitive urinary spaces), the loss of apical cell junctions, and the development of a capillary network with its associated mesangium. With the extension of the capillaries and mesangium between the proximal ends of adjacent undifferentiated nephrons, visceral epithelial cells (podocytes), with long cell processes (trabeculae) and slit membranes, make their appearance. The urinary spaces resulting from this form of development are lined by the epithelium of the dilated ends of the nephrons (nephric capsules). The cells of these capsules differentiate mainly into podocytes, but a few parietal cells connect to the draining tubule. This method of development explains the unique form of the renal corpuscle in the adult lamprey. Despite the type of morphogenesis, this renal corpuscle possesses the fine-structural features seen in the renal corpuscles of other vertebrates.", "contents": "Development of the renal corpuscle during metamorphosis in the lamprey. The renal corpuscle of the adult lamprey, Petromyzon marinus L., is formed during the programmed period of metamorphosis. Development is initiated early in this metamorphic period and is marked by the synchronous formation and growth of rudimentary nephron units (RNU) from longitudinal cord of nephrogenictissue extending from the posterior tip of the degenerating larval kidney to the cloaca and connected to the peritoneal epithelium. Detachment of the RNU from the peritoneum involves autolysis and cell death and is accompanied by their branching into five or six hexagonally-arranged nephrons which radiate from the original point of attachment. Differentiation of the epithelial cells at the proximal ends of the nephrons is preceded by the widening of lateral intercellular spaces, the formation of tubular lumina (primitive urinary spaces), the loss of apical cell junctions, and the development of a capillary network with its associated mesangium. With the extension of the capillaries and mesangium between the proximal ends of adjacent undifferentiated nephrons, visceral epithelial cells (podocytes), with long cell processes (trabeculae) and slit membranes, make their appearance. The urinary spaces resulting from this form of development are lined by the epithelium of the dilated ends of the nephrons (nephric capsules). The cells of these capsules differentiate mainly into podocytes, but a few parietal cells connect to the draining tubule. This method of development explains the unique form of the renal corpuscle in the adult lamprey. Despite the type of morphogenesis, this renal corpuscle possesses the fine-structural features seen in the renal corpuscles of other vertebrates."}
{"id": "PMID:474446", "title": "The association of amorphous mineral deposits with the plasma membrane of pre- and young odontoblasts and their relationship to the origin of dentinal matrix vesicles in rat incisor teeth.", "content": "Young and preodontoblasts and matrix vesicles which occur in the presecretory region of incisor teeth of growing rats were examined in stained and unstained ultrathin sections in order to characterize sites involved in the initial mineralization of dentin. Common to pre- and young odontoblasts in the presecretory region were hemispherical membrane-associated amorphous densities, measuring 5-35 nm in diameter after fixation in glutaraldehyde-osmium tetroxide or glutaraldehyde only. Amorphous densities were associated also with the limiting membranes of some vesicles in the extracellular matrix. Other vesicles in the extracellular matrix contained needle-like crystalline deposits typical of dentinal matrix vesicles. Fully differentiated odontoblasts in more incisal regions of the tooth lacked plasma membrane-associated amorphous densities. Neither amorphous nor crystalline densities were associated with any other cellular or subcellular structures in cells of the presecretory region. Flotation of ultrathin sections on solutions of EDTA or EGTA removed the amorphous densities from the plasma membranes, suggesting that the amorphous densities are calcium-containing mineral deposits. Amorphous deposits were associated with the membrane of vesicular structures protruding from the surfaces of pre- and young odontoblasts, suggesting that vesicles found in the extracellular matrix arise by budding from the plasma membranes of pre- and young odontoblasts. The occurrence of amorphous mineral deposits in association with the limiting membrane of some vesicles in the extracellular matrix, and the occurrence of needle-like mineral crystals within other matrix vesicles, suggest that an amorphous-to-crystalline phase transformation of mineral takes place within the matrix vesicle. The results of this study suggest that calcium-binding sites associated with plasma membranes of pre- and young odontoblasts act as nucleating centers for primary mineral deposition in tooth dentin.", "contents": "The association of amorphous mineral deposits with the plasma membrane of pre- and young odontoblasts and their relationship to the origin of dentinal matrix vesicles in rat incisor teeth. Young and preodontoblasts and matrix vesicles which occur in the presecretory region of incisor teeth of growing rats were examined in stained and unstained ultrathin sections in order to characterize sites involved in the initial mineralization of dentin. Common to pre- and young odontoblasts in the presecretory region were hemispherical membrane-associated amorphous densities, measuring 5-35 nm in diameter after fixation in glutaraldehyde-osmium tetroxide or glutaraldehyde only. Amorphous densities were associated also with the limiting membranes of some vesicles in the extracellular matrix. Other vesicles in the extracellular matrix contained needle-like crystalline deposits typical of dentinal matrix vesicles. Fully differentiated odontoblasts in more incisal regions of the tooth lacked plasma membrane-associated amorphous densities. Neither amorphous nor crystalline densities were associated with any other cellular or subcellular structures in cells of the presecretory region. Flotation of ultrathin sections on solutions of EDTA or EGTA removed the amorphous densities from the plasma membranes, suggesting that the amorphous densities are calcium-containing mineral deposits. Amorphous deposits were associated with the membrane of vesicular structures protruding from the surfaces of pre- and young odontoblasts, suggesting that vesicles found in the extracellular matrix arise by budding from the plasma membranes of pre- and young odontoblasts. The occurrence of amorphous mineral deposits in association with the limiting membrane of some vesicles in the extracellular matrix, and the occurrence of needle-like mineral crystals within other matrix vesicles, suggest that an amorphous-to-crystalline phase transformation of mineral takes place within the matrix vesicle. The results of this study suggest that calcium-binding sites associated with plasma membranes of pre- and young odontoblasts act as nucleating centers for primary mineral deposition in tooth dentin."}
{"id": "PMID:474447", "title": "The prism pattern of rat molar enamel: a scanning electron microscope study.", "content": "Rat molar enamel has been studied by sectioning the enamel along various planes, and observing the etched surfaces in the SEM. It was found that the prism pattern was much more variable than in rat incisor enamel. Regions without prism decussation seemed to dominate in the occlusal half of the molars. Where present, prism decussation was of the uniserial lamellar type, but it varied considerably in distribution, extent, and distinctness. Prism decussation seemed to have a predilection for the cervical enamel, and was almost absent in the enamel on the occlusal surface. The interprismatic substance showed a characteristic configuration: In the inner enamel it appeared in the form of radially oriented sheets, which tended to delimit radially directed, single lines of prisms. In regions with prism decussation these single lines of prisms encompassed prisms belonging to different prism lamellae. In the outer part of the enamel the interprismatic substance exhibited a honeycomb appearance. The similarities and differences between the prism patterns of rat incisor and molar enamel may be of importance for understanding the mechanisms of amelogenesis, especially for the recognition of factors controlling the movement of ameloblasts.", "contents": "The prism pattern of rat molar enamel: a scanning electron microscope study. Rat molar enamel has been studied by sectioning the enamel along various planes, and observing the etched surfaces in the SEM. It was found that the prism pattern was much more variable than in rat incisor enamel. Regions without prism decussation seemed to dominate in the occlusal half of the molars. Where present, prism decussation was of the uniserial lamellar type, but it varied considerably in distribution, extent, and distinctness. Prism decussation seemed to have a predilection for the cervical enamel, and was almost absent in the enamel on the occlusal surface. The interprismatic substance showed a characteristic configuration: In the inner enamel it appeared in the form of radially oriented sheets, which tended to delimit radially directed, single lines of prisms. In regions with prism decussation these single lines of prisms encompassed prisms belonging to different prism lamellae. In the outer part of the enamel the interprismatic substance exhibited a honeycomb appearance. The similarities and differences between the prism patterns of rat incisor and molar enamel may be of importance for understanding the mechanisms of amelogenesis, especially for the recognition of factors controlling the movement of ameloblasts."}
{"id": "PMID:474448", "title": "Observations on the inter-relationships of Sertoli cells at the level of the blood- testis barrier: evidence for formation and resorption of Sertoli-Sertoli tubulobulbar complexes during the spermatogenic cycle of the rat.", "content": "Structures termed tubulobulbar complexes are known to be formed by adjoining Sertoli cells at the level of the blood-testis barrier (Russell and Clermont, '76). Here, long (2-4 micrometer) tubular evaginations of one Sertoli cell, which end in bulbous dilations, are seen in corresponding invaginations of a neighboring Sertoli cell. In most regions of the tubular and bulbous portions of the complex, the Sertoli plasma membranes were found to be separated by a 4-5-nm intercellular space, but in some areas the membranes converged to form tight and gap junctions. The numbers, distribution and properties of tubulobulbar complexes were studied in relation to the cycle of the seminiferous epithelium. From the data obtained it was concluded that tubulobulbar complexes develop and undergo regressive changes during the spermatogenic cycle. Most complexes arise during the early stages of the cycle (Stages II-V) and develop large bulbous endings. Developing tubulobulbar complexes consist of short evaginations of one Sertoli cell which face a bristle-coated pit of the opposing Sertoli cell. At midcycle (Stages VI-VII) most show regressive changes and are eventually resorbed as a consequence of the action of nearby Sertoli lysosomes. Once resorbed, the probability of seeing a tubulobulbar complex in thin sections decreases from 4- to 8-fold. The few tubulobulbar complexes which remain past this period (Stages VII-XIV-I) usually lack bulbous endings and are fequently seen above type A spermatogonia. The data suggest that small fragments of cytoplasm and plasma membrane (including junctional surfaces) are lost from one Sertoli cell as a result of the degradative processes occurring in a neighboring Sertoli cell. Tubulobulbar resorption is discussed in relation to the impending breakdown of the blood-testis barrier above spermatocytes as these cells move upward. The possible significance of the cyclic resorption of tight and gap junctional sites between Sertoli cells is also discussed.", "contents": "Observations on the inter-relationships of Sertoli cells at the level of the blood- testis barrier: evidence for formation and resorption of Sertoli-Sertoli tubulobulbar complexes during the spermatogenic cycle of the rat. Structures termed tubulobulbar complexes are known to be formed by adjoining Sertoli cells at the level of the blood-testis barrier (Russell and Clermont, '76). Here, long (2-4 micrometer) tubular evaginations of one Sertoli cell, which end in bulbous dilations, are seen in corresponding invaginations of a neighboring Sertoli cell. In most regions of the tubular and bulbous portions of the complex, the Sertoli plasma membranes were found to be separated by a 4-5-nm intercellular space, but in some areas the membranes converged to form tight and gap junctions. The numbers, distribution and properties of tubulobulbar complexes were studied in relation to the cycle of the seminiferous epithelium. From the data obtained it was concluded that tubulobulbar complexes develop and undergo regressive changes during the spermatogenic cycle. Most complexes arise during the early stages of the cycle (Stages II-V) and develop large bulbous endings. Developing tubulobulbar complexes consist of short evaginations of one Sertoli cell which face a bristle-coated pit of the opposing Sertoli cell. At midcycle (Stages VI-VII) most show regressive changes and are eventually resorbed as a consequence of the action of nearby Sertoli lysosomes. Once resorbed, the probability of seeing a tubulobulbar complex in thin sections decreases from 4- to 8-fold. The few tubulobulbar complexes which remain past this period (Stages VII-XIV-I) usually lack bulbous endings and are fequently seen above type A spermatogonia. The data suggest that small fragments of cytoplasm and plasma membrane (including junctional surfaces) are lost from one Sertoli cell as a result of the degradative processes occurring in a neighboring Sertoli cell. Tubulobulbar resorption is discussed in relation to the impending breakdown of the blood-testis barrier above spermatocytes as these cells move upward. The possible significance of the cyclic resorption of tight and gap junctional sites between Sertoli cells is also discussed."}
{"id": "PMID:474449", "title": "Maturation of rabbit auricular chondrocytes grown in vitro in monolayer culture.", "content": "Chondrocytes isolated from auricular cartilage of 7-day-old rabbits were grown in vitro until the onset of phase III, occurring after 10-14 population doublings (PD). The size of cells and their dry mass were measured at various PD levels. These data were compared with results of analogous measurements of chondrocytes freshly isolated from 28-day-old rabbits. Both in vivo, during cartilage growth, and in vitro, some of the chondrocytes increased considerably in size and acquired two nuclei. Chondrocytes cultured in vitro for 4 population doublings were still capable of depositing elastic fibers in culture and forming cartilage after intramuscular transplantation. After longer periods of cultivation the ability of cells to produce a cartilage matrix declined. It is suggested that the auricular chondrocytes may represent a convenient model for comparative studies of cell aging in culture and in vivo, owing to the simplicity of matching senescent cells arising in both these situations.", "contents": "Maturation of rabbit auricular chondrocytes grown in vitro in monolayer culture. Chondrocytes isolated from auricular cartilage of 7-day-old rabbits were grown in vitro until the onset of phase III, occurring after 10-14 population doublings (PD). The size of cells and their dry mass were measured at various PD levels. These data were compared with results of analogous measurements of chondrocytes freshly isolated from 28-day-old rabbits. Both in vivo, during cartilage growth, and in vitro, some of the chondrocytes increased considerably in size and acquired two nuclei. Chondrocytes cultured in vitro for 4 population doublings were still capable of depositing elastic fibers in culture and forming cartilage after intramuscular transplantation. After longer periods of cultivation the ability of cells to produce a cartilage matrix declined. It is suggested that the auricular chondrocytes may represent a convenient model for comparative studies of cell aging in culture and in vivo, owing to the simplicity of matching senescent cells arising in both these situations."}
{"id": "PMID:474450", "title": "The superior olivary complex in C57BL/6 mice.", "content": "The cellular and cytoarchitectural features of the lateral superior olive, the medial superior olive, the superior paraolivary nucleus and the medial, lateral and ventral nuclei of the trapezoid body are described in C57BL/6 mice using Nissl, Bodian and Golgi techniques. Principal, spindle and marginal cells are present in a well-defined lateral superior olive. The dendrites of these cells run primarily within rostrocaudal sheets as in the cat. The principal cells of the medial nucleus of the trapezoid body are similar to the principal cells in the cat. Large multipolar cells characterize the lateral nucleus of the trapezoid body and bipolar cells with a medial-lateral orientation are found in the medial superior olive. The largest neurons are found in the superior paraolivary nucleus and the lateral superior olive, and the medial and ventral nuclei of the trapezoid body. While brain weight and neuronal packing density change with development, the characteristic location of cell groups and the shape and Nissl-staining pattern of neurons in the youngest brains examined were essentially unchanged in the adult mice, although dendritic maturation had occurred. The homologies of the C57BL/6 superior olivary complex nuclei with the same areas described in other mouse strains, rat and cat are discussed. This study expands our understanding of the organization of the superior olivary complex in an inbred strain of Mus musculus and relates it to other species. The data about changes occurring during postnatal maturation may aid in the interpretation of behavioral and physiological studies of neonatal plasticity of the auditory system.", "contents": "The superior olivary complex in C57BL/6 mice. The cellular and cytoarchitectural features of the lateral superior olive, the medial superior olive, the superior paraolivary nucleus and the medial, lateral and ventral nuclei of the trapezoid body are described in C57BL/6 mice using Nissl, Bodian and Golgi techniques. Principal, spindle and marginal cells are present in a well-defined lateral superior olive. The dendrites of these cells run primarily within rostrocaudal sheets as in the cat. The principal cells of the medial nucleus of the trapezoid body are similar to the principal cells in the cat. Large multipolar cells characterize the lateral nucleus of the trapezoid body and bipolar cells with a medial-lateral orientation are found in the medial superior olive. The largest neurons are found in the superior paraolivary nucleus and the lateral superior olive, and the medial and ventral nuclei of the trapezoid body. While brain weight and neuronal packing density change with development, the characteristic location of cell groups and the shape and Nissl-staining pattern of neurons in the youngest brains examined were essentially unchanged in the adult mice, although dendritic maturation had occurred. The homologies of the C57BL/6 superior olivary complex nuclei with the same areas described in other mouse strains, rat and cat are discussed. This study expands our understanding of the organization of the superior olivary complex in an inbred strain of Mus musculus and relates it to other species. The data about changes occurring during postnatal maturation may aid in the interpretation of behavioral and physiological studies of neonatal plasticity of the auditory system."}
{"id": "PMID:474451", "title": "Successful maintenance of suckling rat ileum in organ culture.", "content": "Ileum from rats 4, 9, 11, 12, and 15 days old can best be maintained for 24 hours in a system using Hanks' Balanced Salt Solution without fetal calf serum, at 25 degrees C and 21% O2. Suckling rat duodenum and jejunum were difficult to maintain well for 24 hours in this system or a variety of other systems that were tried. A temperature of 37 degrees C hastened deterioration of duodenum, jejunum or ileum. With ileum, 3H-thymidine and 14C-leucine were increasingly incorporated into DNA and protein over the 24-hour period. Light microscopy, as well as scanning and transmission electron microscopy, showed very good preservation of the ileum after 24 hours. The addition to the medium of hydrocortisone, 1 micron, and thyroxine, 0.01 micron, alone or in combination, did not change DNA or protein synthesis, or morphology, possibly because of the relatively short (24 hour) time period. Our organ culture system emphasizes the differences between suckling rat ileum and the rest of the intestine, and provides a new tool for evaluating, over a 24-hour period, the developing rat small intestine.", "contents": "Successful maintenance of suckling rat ileum in organ culture. Ileum from rats 4, 9, 11, 12, and 15 days old can best be maintained for 24 hours in a system using Hanks' Balanced Salt Solution without fetal calf serum, at 25 degrees C and 21% O2. Suckling rat duodenum and jejunum were difficult to maintain well for 24 hours in this system or a variety of other systems that were tried. A temperature of 37 degrees C hastened deterioration of duodenum, jejunum or ileum. With ileum, 3H-thymidine and 14C-leucine were increasingly incorporated into DNA and protein over the 24-hour period. Light microscopy, as well as scanning and transmission electron microscopy, showed very good preservation of the ileum after 24 hours. The addition to the medium of hydrocortisone, 1 micron, and thyroxine, 0.01 micron, alone or in combination, did not change DNA or protein synthesis, or morphology, possibly because of the relatively short (24 hour) time period. Our organ culture system emphasizes the differences between suckling rat ileum and the rest of the intestine, and provides a new tool for evaluating, over a 24-hour period, the developing rat small intestine."}
{"id": "PMID:474460", "title": "Nutrition labeling statement. The Board of the National Nutrition Consortium.", "content": "The National Nutrition Consortium Board commends the FDA for its efforts to provide nutrition information to the consumer. We emphasize the necessity of supporting this system with a comprehensive nutrition education program. The system should lend itself to providing as much information as is possible about the total food supply. Changes in the current system must be based on well-designed and carefully analyzed consumer research data.", "contents": "Nutrition labeling statement. The Board of the National Nutrition Consortium. The National Nutrition Consortium Board commends the FDA for its efforts to provide nutrition information to the consumer. We emphasize the necessity of supporting this system with a comprehensive nutrition education program. The system should lend itself to providing as much information as is possible about the total food supply. Changes in the current system must be based on well-designed and carefully analyzed consumer research data."}
{"id": "PMID:474465", "title": "Suppression of food intake by adenosine and inosine.", "content": "The effect of adenosine and inosine on food consumption was tested by a 4 hr satiety assay in rats fasted overnight who then received injections subcutaneously, and also in ad libitum fed rats infused intravenously for 24 hr periods. It was found that adenosine, and to a lesser degree inosine, produced a significant suppression of food intake (86 and 69% reduction from control, respectively) during the 1st hr after subbcutaneous injection. This effect persisted for 4 hr, at the end of which both substances produced an average 37% reduction in food intake from control values. This effect of adenosine was also found in ad libitum-fed animals infused intravenously with adenosine for 24 hr (35% reduction from control). These studies report a previously undescribed effect of adenosine and add another substance to the list of agents shown to influence food intake regulation. Since adenosine is known to play a local regulatory role on adipose tissue metabolism and is generated and released from adipose tissue, the present findings may indicate an additional role for adenosine in providing a feedback regulatory signal between the adipose organ and the hypothalamic centers of food intake regulation.", "contents": "Suppression of food intake by adenosine and inosine. The effect of adenosine and inosine on food consumption was tested by a 4 hr satiety assay in rats fasted overnight who then received injections subcutaneously, and also in ad libitum fed rats infused intravenously for 24 hr periods. It was found that adenosine, and to a lesser degree inosine, produced a significant suppression of food intake (86 and 69% reduction from control, respectively) during the 1st hr after subbcutaneous injection. This effect persisted for 4 hr, at the end of which both substances produced an average 37% reduction in food intake from control values. This effect of adenosine was also found in ad libitum-fed animals infused intravenously with adenosine for 24 hr (35% reduction from control). These studies report a previously undescribed effect of adenosine and add another substance to the list of agents shown to influence food intake regulation. Since adenosine is known to play a local regulatory role on adipose tissue metabolism and is generated and released from adipose tissue, the present findings may indicate an additional role for adenosine in providing a feedback regulatory signal between the adipose organ and the hypothalamic centers of food intake regulation."}
{"id": "PMID:474466", "title": "Nutritional deprivation in childhood and the body size, activity, and physical work capacity of young boys.", "content": "Clinical nutritional status and physical work capacity at submaximal level were assessed in 96 rural Hyderbad boys, 14 to 17 years of age. Data available on the previous nutritional status of these children when they were 5 years of age were used for classifying them into different nutritional grades. Factors responsible for undernutrition childhood continued to operate on later growth. Both height and weight at 5 years correlated well (P less than 0.001) with current height and weight. About 64% (P less than 0.001) of the variation in physical work capacity at a heart rate of 170/min (physical work capacity 170 kpm/min) could be explained by the differences in current body weight; habitual physical activity explained another 10% (P less than 0.001) of the variation. Malnutrition in early childhood continuing into adolescence could be considered to have adversely affected their work capacity by influencing their body weight. However, even severe undernutrition during childhood had no effect on work performance, when expressed in terms of unit weight. But for the same work load, undernourished subjects had to use significantly higher heart rate at moderate work level as compared to their counterparts.", "contents": "Nutritional deprivation in childhood and the body size, activity, and physical work capacity of young boys. Clinical nutritional status and physical work capacity at submaximal level were assessed in 96 rural Hyderbad boys, 14 to 17 years of age. Data available on the previous nutritional status of these children when they were 5 years of age were used for classifying them into different nutritional grades. Factors responsible for undernutrition childhood continued to operate on later growth. Both height and weight at 5 years correlated well (P less than 0.001) with current height and weight. About 64% (P less than 0.001) of the variation in physical work capacity at a heart rate of 170/min (physical work capacity 170 kpm/min) could be explained by the differences in current body weight; habitual physical activity explained another 10% (P less than 0.001) of the variation. Malnutrition in early childhood continuing into adolescence could be considered to have adversely affected their work capacity by influencing their body weight. However, even severe undernutrition during childhood had no effect on work performance, when expressed in terms of unit weight. But for the same work load, undernourished subjects had to use significantly higher heart rate at moderate work level as compared to their counterparts."}
{"id": "PMID:474467", "title": "Effects of a vigorous walking program on body composition, and carbohydrate and lipid metabolism of obese young men.", "content": "With no attempt made to influence their diet, six sedentary obese men ages 19 to 31 completed 16 weeks of vigorous walking 90 min, 5 days/week, on a treadmill at up to 3.2 mph on a 10% grade, expending about 1100 kcal per session. Body composition studies indicated a loss of 5.9 kg of body fat and a gain of 0.2 kg of lean tissue for a net loss of 5.7 kg. Percentage body fat decreased from 23.3 to 17.4. Monitored food intake initially increased, then progressively decreased below pretraining levels. Work capacity and cardiovascular efficiency improved with training. Plasma cholesterol and triglyceride concentrations were not significantly changed; however, high density lipoprotein cholesterol progressively increased to 15.6% above pretraining levels and the high/low density lipoprotein ratio increased 25.9%. Fasting blood sugar was significantly lower after training. Blood glucose concentrations after a glucose challenge did not significantly change, but a 43% reduction in plasma radioimmunoassay insulin levels and a 36% decrease in the ratio of insulin/glucose concentration occurred. Thus, vigorous regular walking resulted in a reduction of body fate stores, endogenous insulin requirements, and food intake, and perhaps improved the ability to eliminate cholestrol by increasing the plasma high density lipoprotein fraction.", "contents": "Effects of a vigorous walking program on body composition, and carbohydrate and lipid metabolism of obese young men. With no attempt made to influence their diet, six sedentary obese men ages 19 to 31 completed 16 weeks of vigorous walking 90 min, 5 days/week, on a treadmill at up to 3.2 mph on a 10% grade, expending about 1100 kcal per session. Body composition studies indicated a loss of 5.9 kg of body fat and a gain of 0.2 kg of lean tissue for a net loss of 5.7 kg. Percentage body fat decreased from 23.3 to 17.4. Monitored food intake initially increased, then progressively decreased below pretraining levels. Work capacity and cardiovascular efficiency improved with training. Plasma cholesterol and triglyceride concentrations were not significantly changed; however, high density lipoprotein cholesterol progressively increased to 15.6% above pretraining levels and the high/low density lipoprotein ratio increased 25.9%. Fasting blood sugar was significantly lower after training. Blood glucose concentrations after a glucose challenge did not significantly change, but a 43% reduction in plasma radioimmunoassay insulin levels and a 36% decrease in the ratio of insulin/glucose concentration occurred. Thus, vigorous regular walking resulted in a reduction of body fate stores, endogenous insulin requirements, and food intake, and perhaps improved the ability to eliminate cholestrol by increasing the plasma high density lipoprotein fraction."}
{"id": "PMID:474468", "title": "Adipose tissue cellularity in obese nondiabetic men in an urbanized Pacific island (Polynesian) population.", "content": "In a Pacific island (Polynesian) population exposed to Westernized food-stuffs and sedentary life during adulthood, the fat cell size in the gluteal region in obese men was 71% higher than in age-matched nonobese controls. Rough estimations of body fat from anthropometric measurements suggest that the obesity of these men were mainly due to fat cell enlargement. Increased blood glucose and plasma lipids were associated with the obesity.", "contents": "Adipose tissue cellularity in obese nondiabetic men in an urbanized Pacific island (Polynesian) population. In a Pacific island (Polynesian) population exposed to Westernized food-stuffs and sedentary life during adulthood, the fat cell size in the gluteal region in obese men was 71% higher than in age-matched nonobese controls. Rough estimations of body fat from anthropometric measurements suggest that the obesity of these men were mainly due to fat cell enlargement. Increased blood glucose and plasma lipids were associated with the obesity."}
{"id": "PMID:474469", "title": "Changes in some lipid variables in obese women during the early days of fasting.", "content": "Adult obese human subjects with a normal or slightly disturbed oral glucose tolerance test, were submitted to a 7-day fast. Initial serum triglyceride levels were inversely related to the Intralipid fractional removal rate, reflecting the close dependency of triglyceridemia on clearance efficiency; the correlation was less significant on the 5th day of fasting. The direction of the change in triglycerides during fasting was clearly related to the prefast triglyceride levels, the groups of patients with a lower level showing an increase, the group with a higher level showing a decrease. Changes in triglyceridemia were inversely related to fractional removal rate; changes in lipid clearing from plasma, however, could not explain the direction of changes in triglyceridemia in all subjects investigated. The group of patients responding to fasting with a decrease in triglyceridemia had a lower mean initial serum cholesterol value and their serum cholesterol levels showed a less prolonged increase during fasting than the other groups of patients.", "contents": "Changes in some lipid variables in obese women during the early days of fasting. Adult obese human subjects with a normal or slightly disturbed oral glucose tolerance test, were submitted to a 7-day fast. Initial serum triglyceride levels were inversely related to the Intralipid fractional removal rate, reflecting the close dependency of triglyceridemia on clearance efficiency; the correlation was less significant on the 5th day of fasting. The direction of the change in triglycerides during fasting was clearly related to the prefast triglyceride levels, the groups of patients with a lower level showing an increase, the group with a higher level showing a decrease. Changes in triglyceridemia were inversely related to fractional removal rate; changes in lipid clearing from plasma, however, could not explain the direction of changes in triglyceridemia in all subjects investigated. The group of patients responding to fasting with a decrease in triglyceridemia had a lower mean initial serum cholesterol value and their serum cholesterol levels showed a less prolonged increase during fasting than the other groups of patients."}
{"id": "PMID:474470", "title": "Comparative effects of alfalfa saponins and alfalfa fiber on cholesterol absorption in rats.", "content": "Intestinal absorption of cholesterol was measured in control rats fed semipurified diets and in rats fed alfalfa meal, in which saponins had been previously extracted, or this extracted material plus alfalfa saponins. A dose of 2 mg radioactive cholesterol was administered intragastrically, and fecal excretion of labeled neutral steroids measured. Absorption of cholesterol was about 76% in control animals, and about 47% in alfalfa-red rats. Extraction of saponins from alfalfa eliminated the cholesterol absorption-lowering effect, while addition of 0.26% alfalfa saponins to the extracted alfalfa restored its activity. The results demonstrate that alfalfa saponins are responsible for the effect of alfalfa meal in reducing cholesterol absorption, and that alfalfa fiber is not involved in this activity.", "contents": "Comparative effects of alfalfa saponins and alfalfa fiber on cholesterol absorption in rats. Intestinal absorption of cholesterol was measured in control rats fed semipurified diets and in rats fed alfalfa meal, in which saponins had been previously extracted, or this extracted material plus alfalfa saponins. A dose of 2 mg radioactive cholesterol was administered intragastrically, and fecal excretion of labeled neutral steroids measured. Absorption of cholesterol was about 76% in control animals, and about 47% in alfalfa-red rats. Extraction of saponins from alfalfa eliminated the cholesterol absorption-lowering effect, while addition of 0.26% alfalfa saponins to the extracted alfalfa restored its activity. The results demonstrate that alfalfa saponins are responsible for the effect of alfalfa meal in reducing cholesterol absorption, and that alfalfa fiber is not involved in this activity."}
{"id": "PMID:474471", "title": "Regulation of vitamin B6 metabolism in human red cells.", "content": "The effects of pyridoxine and pyridoxal 5-phosphate (PLP) administration on pyridoxine kinase (PnK) and asparate aminotransferase (EGOT), a PLP-dependent enzyme, were studied in human red cells separated into young and old populations by density centrifugation. After a delay of 48 hr, both pyridoxine and PLP increase EGOT activity in mature red cells by activating preformed GOT apoenzyme. In addition, in young erythroid cells, pyridoxine therapy induces synthesis of PnK, while both pyridoxine and PLP induce synthesis of GOT apoprotein. Thus, PLP stimulates EGOT induction without a change in PnK activity, suggesting that PLP enters erythroid precursor cells without prior dephosphorylation. However, with both pyridoxine and PLP, the full induction of enzyme activities reflect the gradual replacement of circulating red cells by newly formed cells with higher enzyme levels. Therefore, the use of EGOT as a measure of vitamin B6 nutritional status requires recognition of the complexities of intracellular enzyme regulation.", "contents": "Regulation of vitamin B6 metabolism in human red cells. The effects of pyridoxine and pyridoxal 5-phosphate (PLP) administration on pyridoxine kinase (PnK) and asparate aminotransferase (EGOT), a PLP-dependent enzyme, were studied in human red cells separated into young and old populations by density centrifugation. After a delay of 48 hr, both pyridoxine and PLP increase EGOT activity in mature red cells by activating preformed GOT apoenzyme. In addition, in young erythroid cells, pyridoxine therapy induces synthesis of PnK, while both pyridoxine and PLP induce synthesis of GOT apoprotein. Thus, PLP stimulates EGOT induction without a change in PnK activity, suggesting that PLP enters erythroid precursor cells without prior dephosphorylation. However, with both pyridoxine and PLP, the full induction of enzyme activities reflect the gradual replacement of circulating red cells by newly formed cells with higher enzyme levels. Therefore, the use of EGOT as a measure of vitamin B6 nutritional status requires recognition of the complexities of intracellular enzyme regulation."}
{"id": "PMID:474472", "title": "The effect of bacterially produced vitamin B12 analogues (cobamides) on the in vitro absorption of cyanocabalamin.", "content": "An incubation system containing rat jejunoileum was used to determine whether analogues of cyanocabalamin (CNCbl) inhibited the in vitro absorption of CNCbl. The naturally occurring analogues \"pseudo B12,\"  \"factor A,\" and the nonnaturally occurring analogue desdemethyl B12, were produced by guided biosynthesis with Propionibacterium arabinosum. Factor B was produced by acid cleavage of cyanocabalamin. Inhibition of Co57-CNBbl uptake was demonstrated for cold CNCbl and for desdemethyl B12, although statistical significance was achieved only for cold CNCbl at 40X and 100X the molar quantity of the radiolabeled CNCbl. However, a trend towards progressive inhibition of specific CNCbl binding was shown as increasing concentrations of CNCbl or desdemethyl B12 were used. No inhibition of specific CNCbl binding could be demonstrated for any of the naturally occurring cobamides tested.", "contents": "The effect of bacterially produced vitamin B12 analogues (cobamides) on the in vitro absorption of cyanocabalamin. An incubation system containing rat jejunoileum was used to determine whether analogues of cyanocabalamin (CNCbl) inhibited the in vitro absorption of CNCbl. The naturally occurring analogues \"pseudo B12,\"  \"factor A,\" and the nonnaturally occurring analogue desdemethyl B12, were produced by guided biosynthesis with Propionibacterium arabinosum. Factor B was produced by acid cleavage of cyanocabalamin. Inhibition of Co57-CNBbl uptake was demonstrated for cold CNCbl and for desdemethyl B12, although statistical significance was achieved only for cold CNCbl at 40X and 100X the molar quantity of the radiolabeled CNCbl. However, a trend towards progressive inhibition of specific CNCbl binding was shown as increasing concentrations of CNCbl or desdemethyl B12 were used. No inhibition of specific CNCbl binding could be demonstrated for any of the naturally occurring cobamides tested."}
{"id": "PMID:474473", "title": "Bile acid metabolism in ascorbic acid-deficient guinea pigs.", "content": "Sterol balance techniques have been used to determine the effect of short-term ascorbic acid (AA) deprivation on bile acid excretion in the guinea pig. The effects of a brief (2-week) AA deficiency on bile acid pool sizes and the activity of the rate controlling enzyme in bile acid biosynthesis have been determined. It was found that, while food intake and body weight were not affected by the short-term AA deficiency, liver AA levels had fallen to 25% of control levels. At the same time, the rate of excretion of bile acids and the size of the bile acid pool were both reduced by about 50% in guinea pigs deficient in AA. These results were supported by a decrease in the activity of cholesterol 7 alpha-hydroxylase in the deficient animals. It is concluded that an AA deficiency will significantly impair bile acid metabolism independent of any side effects of clinical scurvy.", "contents": "Bile acid metabolism in ascorbic acid-deficient guinea pigs. Sterol balance techniques have been used to determine the effect of short-term ascorbic acid (AA) deprivation on bile acid excretion in the guinea pig. The effects of a brief (2-week) AA deficiency on bile acid pool sizes and the activity of the rate controlling enzyme in bile acid biosynthesis have been determined. It was found that, while food intake and body weight were not affected by the short-term AA deficiency, liver AA levels had fallen to 25% of control levels. At the same time, the rate of excretion of bile acids and the size of the bile acid pool were both reduced by about 50% in guinea pigs deficient in AA. These results were supported by a decrease in the activity of cholesterol 7 alpha-hydroxylase in the deficient animals. It is concluded that an AA deficiency will significantly impair bile acid metabolism independent of any side effects of clinical scurvy."}
{"id": "PMID:474474", "title": "The effect of dietary fiber on the postprandial serum vitamin A concentration in man.", "content": "In 11 normal female subjects, ages 19 to 22 years, the postprandial serum vitamin A concentration was measured 3, 5, 7, and 9 hr after oral administration 300,000 IU vitamin A-palmitate given with a formula diet to which was added 40 g wheat bran, 40 g microcrystalline cellulose, 15 g apple pectin, 15 g guar flour, 15 g carob bean flour, or 20 g carrageenan. When vitamin A was given together with guar flour and apple pectin 3 hr after starting the experiment, the mean serum vitamin A concentration was significantly higher than in controls. Areas under serum vitamin A concentration curves, taken as a measure of the amount of vitamin A absorbed, were significantly higher when the vitamin was administered together with the investigated dietary fiber substances.", "contents": "The effect of dietary fiber on the postprandial serum vitamin A concentration in man. In 11 normal female subjects, ages 19 to 22 years, the postprandial serum vitamin A concentration was measured 3, 5, 7, and 9 hr after oral administration 300,000 IU vitamin A-palmitate given with a formula diet to which was added 40 g wheat bran, 40 g microcrystalline cellulose, 15 g apple pectin, 15 g guar flour, 15 g carob bean flour, or 20 g carrageenan. When vitamin A was given together with guar flour and apple pectin 3 hr after starting the experiment, the mean serum vitamin A concentration was significantly higher than in controls. Areas under serum vitamin A concentration curves, taken as a measure of the amount of vitamin A absorbed, were significantly higher when the vitamin was administered together with the investigated dietary fiber substances."}
{"id": "PMID:474476", "title": "Saliva zinc levels: potential indicators of zinc status.", "content": "Mixed saliva was collected from eight adolescent females participating in a metabolic study and from 59 adolescent females participating in a nutrition survey. Zinc concentrations, protein concentrations, and zinc:protein ratios of whole mixed saliva samples and of supernatant samples, which were prepared from the mixed saliva, were determined. Zinc analyses were performed with an atomic absorption spectrophotometer equipped with a graphite furnace. Significantly (P less than 0.05) lower levels of zinc were found in the supernant samples, but not in the whole saliva samples, when the same subjects were fed 11.5 mg zinc daily rather than 14.7 mg zinc daily during the metabolic study. The mean zinc concentrations of the whole saliva and the supernatant samples collected during the survey were 173 +/- 94 (SD) and 30.5 +/- 14.8 ng/ml, respectively. The levels of zinc and protein in the whole mixed saliva (r = 0.460, P less than 0.001) and the supernatant samples (4 = 0.486, P less than 0.001) were correlated. The levels of zinc in both types of saliva samples were not correlated to serum or hair zinc levels of girls in the survey.", "contents": "Saliva zinc levels: potential indicators of zinc status. Mixed saliva was collected from eight adolescent females participating in a metabolic study and from 59 adolescent females participating in a nutrition survey. Zinc concentrations, protein concentrations, and zinc:protein ratios of whole mixed saliva samples and of supernatant samples, which were prepared from the mixed saliva, were determined. Zinc analyses were performed with an atomic absorption spectrophotometer equipped with a graphite furnace. Significantly (P less than 0.05) lower levels of zinc were found in the supernant samples, but not in the whole saliva samples, when the same subjects were fed 11.5 mg zinc daily rather than 14.7 mg zinc daily during the metabolic study. The mean zinc concentrations of the whole saliva and the supernatant samples collected during the survey were 173 +/- 94 (SD) and 30.5 +/- 14.8 ng/ml, respectively. The levels of zinc and protein in the whole mixed saliva (r = 0.460, P less than 0.001) and the supernatant samples (4 = 0.486, P less than 0.001) were correlated. The levels of zinc in both types of saliva samples were not correlated to serum or hair zinc levels of girls in the survey."}
{"id": "PMID:474477", "title": "Metabolic balances of cadmium, copper, manganese, and zinc in man.", "content": "Balance studies of cadmium, copper, manganese, and zine were carried out under constant dietary conditions in eight adult males during two calcium intake levels of 200 and 800 mg/day and in an additional single case during a calcium intake of 1500 mg/day. The dietary content and the excretions of these elements in urine and stool were determined. The mean dietary content of cadmium was 32.9 micrograms/day, of copper 1020 micrograms/day, of manganese 2130 micrograms/day, and of zinc 12.4 mg/day. The ratio of the fecal/urinary cadmium excretion was approximately 1.5 and the main pathway of excretion of the other three elements was via the intestine, while the urinary excretions were very low. The different trace element balances were either slightly negative or in equilibrium, except that the zinc balances was positive in 50% of the cases. All balances should be considered maximal values, as the losses in sweat were not determined. The calcium intake level had little effect on the excretion and retention of these trace elements.", "contents": "Metabolic balances of cadmium, copper, manganese, and zinc in man. Balance studies of cadmium, copper, manganese, and zine were carried out under constant dietary conditions in eight adult males during two calcium intake levels of 200 and 800 mg/day and in an additional single case during a calcium intake of 1500 mg/day. The dietary content and the excretions of these elements in urine and stool were determined. The mean dietary content of cadmium was 32.9 micrograms/day, of copper 1020 micrograms/day, of manganese 2130 micrograms/day, and of zinc 12.4 mg/day. The ratio of the fecal/urinary cadmium excretion was approximately 1.5 and the main pathway of excretion of the other three elements was via the intestine, while the urinary excretions were very low. The different trace element balances were either slightly negative or in equilibrium, except that the zinc balances was positive in 50% of the cases. All balances should be considered maximal values, as the losses in sweat were not determined. The calcium intake level had little effect on the excretion and retention of these trace elements."}
{"id": "PMID:474478", "title": "Effect of fiber from fruits and vegetables on metabolic responses of human subjects, II. Calcium, magnesium, iron, and silicon balances.", "content": "Intakes and excretions of calcium, magnesium, iron, and silicon were determined for 12 men consuming controlled diets containing fruits and vegetables (high fiber diet) or fruit and vegetable juices (low fiber diet) for periods of 26 days. Magnesium and iron were added to the low fiber diet to make the two diets as equivalent as possible in these respects. Mean daily intakes on the low and high fiber diets, respectively, were: calcium, 1070 and 1166 mg; magnesium, 355 and 322 mg; iron, 21.8 and 26.4 mg; silicon, 21.6 and 45.8 mg. Mean daily balances on the low and high fiber diets repectively, were: calcium +72 and -122 mg; magnesium, +28 and -32 mg; iron, +3.8 and +4.6 mg; silicon, -3.5 and -14.6 mg. Calcium magnesium, and silicon balances were significantly lower on the high than on the low fiber diet (P less than 0.01).", "contents": "Effect of fiber from fruits and vegetables on metabolic responses of human subjects, II. Calcium, magnesium, iron, and silicon balances. Intakes and excretions of calcium, magnesium, iron, and silicon were determined for 12 men consuming controlled diets containing fruits and vegetables (high fiber diet) or fruit and vegetable juices (low fiber diet) for periods of 26 days. Magnesium and iron were added to the low fiber diet to make the two diets as equivalent as possible in these respects. Mean daily intakes on the low and high fiber diets, respectively, were: calcium, 1070 and 1166 mg; magnesium, 355 and 322 mg; iron, 21.8 and 26.4 mg; silicon, 21.6 and 45.8 mg. Mean daily balances on the low and high fiber diets repectively, were: calcium +72 and -122 mg; magnesium, +28 and -32 mg; iron, +3.8 and +4.6 mg; silicon, -3.5 and -14.6 mg. Calcium magnesium, and silicon balances were significantly lower on the high than on the low fiber diet (P less than 0.01)."}
{"id": "PMID:474479", "title": "The effect of raw carrot on serum lipids and colon function.", "content": "Two hundred grams of raw carrot eaten at breakfast each day for 3 weeks significantly reduced serum cholesterol by 11%, increased fecal bile acid and fat excretion by 50%, and modestly increased stool weight by 25%. This suggests an associated change in bacterial flora or metabolism. The changes in serum cholesterol, fecal bile acids, and fat persisted 3 weeks after stopping treatment.", "contents": "The effect of raw carrot on serum lipids and colon function. Two hundred grams of raw carrot eaten at breakfast each day for 3 weeks significantly reduced serum cholesterol by 11%, increased fecal bile acid and fat excretion by 50%, and modestly increased stool weight by 25%. This suggests an associated change in bacterial flora or metabolism. The changes in serum cholesterol, fecal bile acids, and fat persisted 3 weeks after stopping treatment."}
{"id": "PMID:474480", "title": "Effect of dietary fiber on copper, zinc, and magnesium utilization by adolescent boys.", "content": "The effect of three dietary fibers on zinc, copper, and magnesium utilization by adolescent boys was studied. The 21-day study was divided into a 2-day introductory nitrogen depletion period, a 3-day adjustment period, and a four 4-day randomly arranged experimental period. During the four experimental periods, the eight adolescent male subjects received a basal diet plus 14.2 g supplements of cellulose, hemicellulose, or pectin. All subjects received all experimental diets. Zinc, copper, and magnesium contents of food, urine and blood serum were measured by atomic absorption spectrophotometry. Relatively small changes in urinary excretion or in blood serum contents of these minerals were found that could be attributed to dietary fiber supplementation. Statistically significant changes in fecal mineral loss were demonstrated. While receiving the basal diet alone or plus hemicellulose, cellulose, or pectin, mean fecal zinc contents (milligrams per subject per day) were 9.64, 13.32, 10.26, and 9.63, respectively; mean fecal copper contents (milligrams per subject per day) were 3.78, 5.04, 4.07, and 3.91, respectively; and mean fecal magnesium contents (milligrams per subject per day) were 303.71, 379.20, 325.69, and 278.48, respectively.", "contents": "Effect of dietary fiber on copper, zinc, and magnesium utilization by adolescent boys. The effect of three dietary fibers on zinc, copper, and magnesium utilization by adolescent boys was studied. The 21-day study was divided into a 2-day introductory nitrogen depletion period, a 3-day adjustment period, and a four 4-day randomly arranged experimental period. During the four experimental periods, the eight adolescent male subjects received a basal diet plus 14.2 g supplements of cellulose, hemicellulose, or pectin. All subjects received all experimental diets. Zinc, copper, and magnesium contents of food, urine and blood serum were measured by atomic absorption spectrophotometry. Relatively small changes in urinary excretion or in blood serum contents of these minerals were found that could be attributed to dietary fiber supplementation. Statistically significant changes in fecal mineral loss were demonstrated. While receiving the basal diet alone or plus hemicellulose, cellulose, or pectin, mean fecal zinc contents (milligrams per subject per day) were 9.64, 13.32, 10.26, and 9.63, respectively; mean fecal copper contents (milligrams per subject per day) were 3.78, 5.04, 4.07, and 3.91, respectively; and mean fecal magnesium contents (milligrams per subject per day) were 303.71, 379.20, 325.69, and 278.48, respectively."}
{"id": "PMID:474481", "title": "Dietary fiber and nutrient intake in Crohn's disease.", "content": "The mean daily intake of dietary fiber, sugar, starch, fat, protein, and total energy was determined in 35 patients with Crohn's disease and 70 normal controls by obtaining individual dietary histories. For each patient with Crohn's disease there two controls, matched for age, sex, and socioeconomic background. In the patients with Crohn's disease the mean dietary fiber intake was established as 26.6 +/- 1.4 g/day, compared to 22.3 +/- 0.9 g/day in the controls, and was thus significantly higher (P less than 0.05). When compared with the controls, the patients with Crohn's disease also exhibited a significantly higher consumption of sugar 156 +/- 14 versus 91 +/- 5 g/day (P less than 0.001), starch (211 +/- 10 versus 170 +/- 9 g/day (P less than 0.01)), and total energy (14.4 +/- 0.7 versus 12.3 +/- 0.5 MJ/ day (P less than 0.01)).", "contents": "Dietary fiber and nutrient intake in Crohn's disease. The mean daily intake of dietary fiber, sugar, starch, fat, protein, and total energy was determined in 35 patients with Crohn's disease and 70 normal controls by obtaining individual dietary histories. For each patient with Crohn's disease there two controls, matched for age, sex, and socioeconomic background. In the patients with Crohn's disease the mean dietary fiber intake was established as 26.6 +/- 1.4 g/day, compared to 22.3 +/- 0.9 g/day in the controls, and was thus significantly higher (P less than 0.05). When compared with the controls, the patients with Crohn's disease also exhibited a significantly higher consumption of sugar 156 +/- 14 versus 91 +/- 5 g/day (P less than 0.001), starch (211 +/- 10 versus 170 +/- 9 g/day (P less than 0.01)), and total energy (14.4 +/- 0.7 versus 12.3 +/- 0.5 MJ/ day (P less than 0.01))."}
{"id": "PMID:474482", "title": "Diet-related toxemia in pregnancy. I. Fat, fatty acids, and cholesterol.", "content": "Toxemia in pregnancy (preeclampsia)is characteristerized by a combination of at least two of the following clinical symptoms: hypertension, edema, and proteinuria. In three successive trials over three consecutive years, the dietary intake of a selected number of young pregnant women attending a Maternal and Infant Care Program at Tuskegee Institute were evaluated for total lipids, individual fatty acids, and cholesterol. Women with toxemia or with any of the individual symptoms were identified and women without toxemia or these symptoms served as controls. Results were variable from repetition to repetition in all but the toxemia group and the edema group. The consumption of total lipids and cholesterol was significantly greater in all three trials by both the toxemia and edema groups. Also, total saturated, monounsaturated, and polyunsaturated fatty acids were eaten in greater amounts. The greatest differences were in palmitic acid, stearic acid, oleic acid, and linoleic acid. The proportion of unsaturated fatty acids consumed in all groups was very low. All differences could be attributed primarily to breakfast and dinner meals and were found in the milk, meat, and egg food groups. Although satistical correlations were found between lipid intake and toxemia of pregnancy any specific relationship between the two is still unclear.", "contents": "Diet-related toxemia in pregnancy. I. Fat, fatty acids, and cholesterol. Toxemia in pregnancy (preeclampsia)is characteristerized by a combination of at least two of the following clinical symptoms: hypertension, edema, and proteinuria. In three successive trials over three consecutive years, the dietary intake of a selected number of young pregnant women attending a Maternal and Infant Care Program at Tuskegee Institute were evaluated for total lipids, individual fatty acids, and cholesterol. Women with toxemia or with any of the individual symptoms were identified and women without toxemia or these symptoms served as controls. Results were variable from repetition to repetition in all but the toxemia group and the edema group. The consumption of total lipids and cholesterol was significantly greater in all three trials by both the toxemia and edema groups. Also, total saturated, monounsaturated, and polyunsaturated fatty acids were eaten in greater amounts. The greatest differences were in palmitic acid, stearic acid, oleic acid, and linoleic acid. The proportion of unsaturated fatty acids consumed in all groups was very low. All differences could be attributed primarily to breakfast and dinner meals and were found in the milk, meat, and egg food groups. Although satistical correlations were found between lipid intake and toxemia of pregnancy any specific relationship between the two is still unclear."}
{"id": "PMID:474483", "title": "Nutritional status of institutionalized and noninstitutionalized aged in Belfast, Northern Ireland.", "content": "A multifaceted analysis of the nutritional status of 196 subjects, aged over 65, institutionalized in hospitals, residential accomodation, and sheltered dwellings and of noninstituionalized subjects was untaken. Subjects of hospital and home, with or without multivitamin supplementation, were grouped separately. The study comprised of 3-day weighed dietary record, biochemical determinations, and clinical examination. The energy intake of females of hospitals and sheltered dwellings was comparatively low. Dietary nutrients most lacking were potassium, magnesium, vitamin D, and vitamin B6. Calcium and vitamin A intake were adequate. Clinical deficiency was rare. Subclinical deficiency was highly prevalent and the deficiency incidence was: anemia 18.6%, ascorbic acid 29.2%, thiamin 13.8%, riboflavin 7.1%, vitamin B6 42.3%, and vitamin D 47.0%. Some kind of mineral or vitamin deficiency was observed biochemically in 91.3% of the nonmultivitamin supplemented group and 64.3% of the multivitamin supplemented group. Regular intake of multivitamin raised the blood levels of riboflavin and ascorbic acid to normal in all, but failed to raise the thiamin and vitamin B6 levels to the normal acceptable levels in 2.9 and 20% of the subjects, respectively. Suggestions are made concerning possibly higher recommended allowance.", "contents": "Nutritional status of institutionalized and noninstitutionalized aged in Belfast, Northern Ireland. A multifaceted analysis of the nutritional status of 196 subjects, aged over 65, institutionalized in hospitals, residential accomodation, and sheltered dwellings and of noninstituionalized subjects was untaken. Subjects of hospital and home, with or without multivitamin supplementation, were grouped separately. The study comprised of 3-day weighed dietary record, biochemical determinations, and clinical examination. The energy intake of females of hospitals and sheltered dwellings was comparatively low. Dietary nutrients most lacking were potassium, magnesium, vitamin D, and vitamin B6. Calcium and vitamin A intake were adequate. Clinical deficiency was rare. Subclinical deficiency was highly prevalent and the deficiency incidence was: anemia 18.6%, ascorbic acid 29.2%, thiamin 13.8%, riboflavin 7.1%, vitamin B6 42.3%, and vitamin D 47.0%. Some kind of mineral or vitamin deficiency was observed biochemically in 91.3% of the nonmultivitamin supplemented group and 64.3% of the multivitamin supplemented group. Regular intake of multivitamin raised the blood levels of riboflavin and ascorbic acid to normal in all, but failed to raise the thiamin and vitamin B6 levels to the normal acceptable levels in 2.9 and 20% of the subjects, respectively. Suggestions are made concerning possibly higher recommended allowance."}
{"id": "PMID:474484", "title": "Lactose intolerance in Iran.", "content": "One hundred five Iranian subjects, ranging in age from 4 months to 25 years, were tested for lactose absorption and tolerance. After ingesting a lactose dose, on the basis of low blood glucose response, 68% of the subjects were malabsorbers. Prevalence of lactose malabsorption increased with age, i.e., 31% of the children less than 3 years of age malabsorbed lactose, whereas 86% of adults did so. Clinical manifestations of lactose intolerance were shown by 39% of all subjects; of those who were lactose malabsorbers, only 57% manifested clinical symptoms. Among the lactose malabsorbers, the lowest prevalence of clinical symptoms occurred among the children less than 3 years of age and the highest in adults.", "contents": "Lactose intolerance in Iran. One hundred five Iranian subjects, ranging in age from 4 months to 25 years, were tested for lactose absorption and tolerance. After ingesting a lactose dose, on the basis of low blood glucose response, 68% of the subjects were malabsorbers. Prevalence of lactose malabsorption increased with age, i.e., 31% of the children less than 3 years of age malabsorbed lactose, whereas 86% of adults did so. Clinical manifestations of lactose intolerance were shown by 39% of all subjects; of those who were lactose malabsorbers, only 57% manifested clinical symptoms. Among the lactose malabsorbers, the lowest prevalence of clinical symptoms occurred among the children less than 3 years of age and the highest in adults."}
{"id": "PMID:474485", "title": "In vivo measurements of body protein based on the determination of nitrogen by prompt gamma analysis.", "content": "Measurement of nitrogen in the human body gives a measure of total protein in the body. Nitrogen (N) may be measured in vivo by a nuclear technique in which the characteristic nitrogen gamma rays emitted after capture of neutrons are detected and counted. The accuracy (as determined by comparison of the measurement of nitrogen in a pig by nuclear and chemical methods) is better than 10%. The reproducibility is less than 6%. The radiation dose is about 5 millirads. This method had been used with 43 patients and 23 normal volunteers and the results are reported. The data are presented in the from of total N content, percentage N by weight, and as a comparison of N and potassium (K) in the body. It is found that the mean percentage of N in the normal males is 2.5%, giving a value of 1.75 kg in the 70-kg standard man. The mean percentage values are not greatly different between normal volunteers and malnourished patients. Taking all subjects, N/K ratios ranged from 7.5 to 20 g/g. Thus K cannot be used as a predictor of N. Nitrogen content of normals correlates well (r = 0.68) with height squared; for malnourished patients there is again a good correlation (r = 0.81) but the regression line for patients is approximately 34% below that for the volunteers consistent with their depleted state.", "contents": "In vivo measurements of body protein based on the determination of nitrogen by prompt gamma analysis. Measurement of nitrogen in the human body gives a measure of total protein in the body. Nitrogen (N) may be measured in vivo by a nuclear technique in which the characteristic nitrogen gamma rays emitted after capture of neutrons are detected and counted. The accuracy (as determined by comparison of the measurement of nitrogen in a pig by nuclear and chemical methods) is better than 10%. The reproducibility is less than 6%. The radiation dose is about 5 millirads. This method had been used with 43 patients and 23 normal volunteers and the results are reported. The data are presented in the from of total N content, percentage N by weight, and as a comparison of N and potassium (K) in the body. It is found that the mean percentage of N in the normal males is 2.5%, giving a value of 1.75 kg in the 70-kg standard man. The mean percentage values are not greatly different between normal volunteers and malnourished patients. Taking all subjects, N/K ratios ranged from 7.5 to 20 g/g. Thus K cannot be used as a predictor of N. Nitrogen content of normals correlates well (r = 0.68) with height squared; for malnourished patients there is again a good correlation (r = 0.81) but the regression line for patients is approximately 34% below that for the volunteers consistent with their depleted state."}
{"id": "PMID:474486", "title": "Lactose malabsorption in Bangladeshi village children: relation with age, history of recent diarrhea, nutritional status, and breast feeding.", "content": "The prevalence of lactose malabsorption (LM) among Bangladeshi village children has been determined using the recent developed breath hydrogen test. Initial hospital-based comparison studies showed general agreement between the breath hydrogen test and a modified lactose tolerance test. Two hundred thirty-four children, stratified by age, nutritional status, and history of recent diarrhea then participated in the field study. LM was diagnosed in more than 80% of children over 36 months of age but in none of the children under 6 months. Rates of LM were significantly increased in children with a history of recent diarrhea and a greater proportion of children in some age groups evidenced malabsorption in association with acute undernutrition. In the weanling age group children who were still breast feeding had a lower rate of LM than fully weaned subjects.", "contents": "Lactose malabsorption in Bangladeshi village children: relation with age, history of recent diarrhea, nutritional status, and breast feeding. The prevalence of lactose malabsorption (LM) among Bangladeshi village children has been determined using the recent developed breath hydrogen test. Initial hospital-based comparison studies showed general agreement between the breath hydrogen test and a modified lactose tolerance test. Two hundred thirty-four children, stratified by age, nutritional status, and history of recent diarrhea then participated in the field study. LM was diagnosed in more than 80% of children over 36 months of age but in none of the children under 6 months. Rates of LM were significantly increased in children with a history of recent diarrhea and a greater proportion of children in some age groups evidenced malabsorption in association with acute undernutrition. In the weanling age group children who were still breast feeding had a lower rate of LM than fully weaned subjects."}
{"id": "PMID:474487", "title": "Myoglobin in myocardial infarction. Results in a coronary-care-unit population.", "content": "The purpose of this study was to define the performance characteristics of serum myoglobin determinations in the diagnosis of acute myocardial infarction (MI). Subjects of the study were 118 patients admitted consecutively to the coronary care unit. Daily measurements of serum myoglobin (by radioimmunoassay) were performed. For patients admitted within 24 hours of the onset of symptoms, a serum myoglobin level of 75 ng/ml had a diagnostic sensitivity of 91% and a specificity of 85%. In the same group of patients, the presence of the myocardial isoenzyme of creatine phosphokinase (CK-MB) showed a sensitivity of 94% and a specificity of 89%. Among the 32 patients with MI, the elevation of myoglobin preceded the appearance of CK-MB in 12 (38%), was concomitant with CK-MB appearance in 15 (47%), and followed CK-MB in none. Given the spectrum of patients in this coronary care unit, the addition of the myoglobin assay to the heart profile has increased its sensitivity for the diagnosis of MI. The assay materials are available in kit form, and the test can be performed in the routine radioimmunoassay laboratory. The results are available for reporting four to five hours after venipuncture.", "contents": "Myoglobin in myocardial infarction. Results in a coronary-care-unit population. The purpose of this study was to define the performance characteristics of serum myoglobin determinations in the diagnosis of acute myocardial infarction (MI). Subjects of the study were 118 patients admitted consecutively to the coronary care unit. Daily measurements of serum myoglobin (by radioimmunoassay) were performed. For patients admitted within 24 hours of the onset of symptoms, a serum myoglobin level of 75 ng/ml had a diagnostic sensitivity of 91% and a specificity of 85%. In the same group of patients, the presence of the myocardial isoenzyme of creatine phosphokinase (CK-MB) showed a sensitivity of 94% and a specificity of 89%. Among the 32 patients with MI, the elevation of myoglobin preceded the appearance of CK-MB in 12 (38%), was concomitant with CK-MB appearance in 15 (47%), and followed CK-MB in none. Given the spectrum of patients in this coronary care unit, the addition of the myoglobin assay to the heart profile has increased its sensitivity for the diagnosis of MI. The assay materials are available in kit form, and the test can be performed in the routine radioimmunoassay laboratory. The results are available for reporting four to five hours after venipuncture."}
{"id": "PMID:474488", "title": "Comparison of the chloride electrode and gravimetric chloride titration sweat tests.", "content": "The chloride electrode sweat test and gravimetric chloride titration technic are compared. The two technics gave comparable mean values, ogives, equivalent points, and overlap for patients with cystic fibrosis and healthy subjects. As a single test with either technic has an unacceptable risk for false-positive or false-negative results, tests should always be done in pairs. A statistical interpretation technic based on prevalence and technical variability of the two tests will decrease the number of undiagnosed subjects and will define probability of cystic fibrosis in the undiagnosed group.", "contents": "Comparison of the chloride electrode and gravimetric chloride titration sweat tests. The chloride electrode sweat test and gravimetric chloride titration technic are compared. The two technics gave comparable mean values, ogives, equivalent points, and overlap for patients with cystic fibrosis and healthy subjects. As a single test with either technic has an unacceptable risk for false-positive or false-negative results, tests should always be done in pairs. A statistical interpretation technic based on prevalence and technical variability of the two tests will decrease the number of undiagnosed subjects and will define probability of cystic fibrosis in the undiagnosed group."}
{"id": "PMID:474489", "title": "Comparison of an automated radioimmunoassay method and a competitive protein-binding method for evaluation of serum cortisol.", "content": "A competitive protein-binding method (CPB) for serum cortisol determination has been compared with a newly developed automated radioimmunoassay (RIA) technic with respect to reproducibility and correlation of patient results. The RIA method uses an immobilized anti-cortisol antibody covalently coupled to the inner surface of a plastic cartridge. A mixture of 3H-labeled and unlabeled cortisol is passed through the cartridge to achieve separation of free and bound cortisol. The bound cortisol is later eluted to regenerate the antibody for the next sample. The RIA procedure had a day-to-day coefficient of variation (CV) of 7% at three cortisol levels. The CPB method had a day-to-day CV of 10% at a concentration of 8 microgram/dl. A comparison of 67 patient samples showed no statistically significant difference between the methods. The regression equation was RIA = 0.96 CPB + 0.14 microgram/dl, with a correlation of 0.92.", "contents": "Comparison of an automated radioimmunoassay method and a competitive protein-binding method for evaluation of serum cortisol. A competitive protein-binding method (CPB) for serum cortisol determination has been compared with a newly developed automated radioimmunoassay (RIA) technic with respect to reproducibility and correlation of patient results. The RIA method uses an immobilized anti-cortisol antibody covalently coupled to the inner surface of a plastic cartridge. A mixture of 3H-labeled and unlabeled cortisol is passed through the cartridge to achieve separation of free and bound cortisol. The bound cortisol is later eluted to regenerate the antibody for the next sample. The RIA procedure had a day-to-day coefficient of variation (CV) of 7% at three cortisol levels. The CPB method had a day-to-day CV of 10% at a concentration of 8 microgram/dl. A comparison of 67 patient samples showed no statistically significant difference between the methods. The regression equation was RIA = 0.96 CPB + 0.14 microgram/dl, with a correlation of 0.92."}
{"id": "PMID:474490", "title": "Evaluation of commercial enzyme reagent kits by use of a semiautomated chemistry analyzer.", "content": "The overall performances of several enzyme reagent kits for alkaline phosphatase, creatine kinase, lactic dehydrogenase, and aspartate aminotransferase were evaluated using an ABA-100 Bichromatic Analyzer. Interassay precision using this instrument with commercial reagents compared well with published data for similar analyses performed at university hospitals and referral laboratories. Significantly poorer precision with lower limits of linearity was observed when reagents recommended for use at 30 C were used at 37 C. Significant differences in measured levels of creatine kinase, lactic dehydrogenase, and aspartate aminotransferase due to different lots of expendable cuvettes were found for elevated levels of these enzymes. All kit reagents met manufacturers' claims for stability; however, different absolute levels of lactic dehydrogenase were observed with one kit reagent on successive days. Slight hemolysis affected creatine kinase levels measured with some reagent kits significantly more than others.", "contents": "Evaluation of commercial enzyme reagent kits by use of a semiautomated chemistry analyzer. The overall performances of several enzyme reagent kits for alkaline phosphatase, creatine kinase, lactic dehydrogenase, and aspartate aminotransferase were evaluated using an ABA-100 Bichromatic Analyzer. Interassay precision using this instrument with commercial reagents compared well with published data for similar analyses performed at university hospitals and referral laboratories. Significantly poorer precision with lower limits of linearity was observed when reagents recommended for use at 30 C were used at 37 C. Significant differences in measured levels of creatine kinase, lactic dehydrogenase, and aspartate aminotransferase due to different lots of expendable cuvettes were found for elevated levels of these enzymes. All kit reagents met manufacturers' claims for stability; however, different absolute levels of lactic dehydrogenase were observed with one kit reagent on successive days. Slight hemolysis affected creatine kinase levels measured with some reagent kits significantly more than others."}
{"id": "PMID:474491", "title": "Quality control by blind sample analysis.", "content": "Quality control efforts may be biased consciously or unconsciously. External programs are handicapped by the time lapse before poor performance is disclosed. Bias induced into internal and external quality control efforts by special handling may be eliminated through submission of blind samples originating from wards, clinics, or phlebotomy areas. Evaluation of laboratory performance is obtained under conditions similar to those experienced by the patients' specimens. Calibration performance by operators, which varies considerably, can be monitored. Laboratory clerical activity can be scrutinized and errors quantitated and corrected. Perhaps most important, the sum of the effects of specimen handling and analytic variability can be determined.", "contents": "Quality control by blind sample analysis. Quality control efforts may be biased consciously or unconsciously. External programs are handicapped by the time lapse before poor performance is disclosed. Bias induced into internal and external quality control efforts by special handling may be eliminated through submission of blind samples originating from wards, clinics, or phlebotomy areas. Evaluation of laboratory performance is obtained under conditions similar to those experienced by the patients' specimens. Calibration performance by operators, which varies considerably, can be monitored. Laboratory clerical activity can be scrutinized and errors quantitated and corrected. Perhaps most important, the sum of the effects of specimen handling and analytic variability can be determined."}
{"id": "PMID:474492", "title": "Transcatheter bronchial brush and forceps biopsies. Histologic evaluation.", "content": "The histologic findings in transcatheter brush and forceps biopsies from 472 cases over a six-year period are evaluated. Diagnostic accuracy based on histologic findings was 38% and based on cytologic findings was 70%. When the two methods of examination were used, however, the overall accuracy was improved to 76%. In Hodgkin's disease and some inflammatory processes histologic examination is essential for the diagnosis. The transcatheter biopsies under fluoroscopic control are especially useful for securing tissues from the peripherally located lesions. When possible, examination of tissue obtained by transcatheter as well as forceps biopsies is encouraged.", "contents": "Transcatheter bronchial brush and forceps biopsies. Histologic evaluation. The histologic findings in transcatheter brush and forceps biopsies from 472 cases over a six-year period are evaluated. Diagnostic accuracy based on histologic findings was 38% and based on cytologic findings was 70%. When the two methods of examination were used, however, the overall accuracy was improved to 76%. In Hodgkin's disease and some inflammatory processes histologic examination is essential for the diagnosis. The transcatheter biopsies under fluoroscopic control are especially useful for securing tissues from the peripherally located lesions. When possible, examination of tissue obtained by transcatheter as well as forceps biopsies is encouraged."}
{"id": "PMID:474493", "title": "Genital mycoplasma infection. Intrauterine infection: pathologic study of the fetus and placenta.", "content": "Mycoplasma infection was present in the fetuses from three spontaneous abortions and in one second-trimester newborn. Gross examination revealed in most cases a severely infected placenta and membranes, with a fetus of normal appearance. The fetal infection presumably followed placental involvement and appeared to have been acquired shortly prior to delivery. Genital mycoplasmas, Ureaplasma urealyticum or Mycoplasma hominis, were isolated from the placentas and the fetal tissues, and from the genital tracts of the mothers. Isolation of mycoplasmas from the liver indicated that bloodstream dissemination of these organisms occurred in the fetus. In the fetus, the pathologic changes were variable. Lesions were identified in the lung by scanning electron microscopy of the bronchial tree in two cases and were accompanied by interstitial pneumonia. An abnormally dilated left ventricle suggestive of cardiomyopathy was observed in one case.", "contents": "Genital mycoplasma infection. Intrauterine infection: pathologic study of the fetus and placenta. Mycoplasma infection was present in the fetuses from three spontaneous abortions and in one second-trimester newborn. Gross examination revealed in most cases a severely infected placenta and membranes, with a fetus of normal appearance. The fetal infection presumably followed placental involvement and appeared to have been acquired shortly prior to delivery. Genital mycoplasmas, Ureaplasma urealyticum or Mycoplasma hominis, were isolated from the placentas and the fetal tissues, and from the genital tracts of the mothers. Isolation of mycoplasmas from the liver indicated that bloodstream dissemination of these organisms occurred in the fetus. In the fetus, the pathologic changes were variable. Lesions were identified in the lung by scanning electron microscopy of the bronchial tree in two cases and were accompanied by interstitial pneumonia. An abnormally dilated left ventricle suggestive of cardiomyopathy was observed in one case."}
{"id": "PMID:474495", "title": "A precision study of a photo-optical factor VIII assay technic.", "content": "Various methods of factor VIII analysis with a wide range of accuracies as confirmed by calculated coefficients of variation have been devised. The authors report a technic of factor VIII assay using a photo-optical clot-detection instrument with commercially available sources of factor VIII-deficient plasma and lyophilized normal control plasma, and compare its accuracy, ease of performance and reliability with those of existing methods. The accuracy of the technic was established by a survey of a normal control group, which revealed a mean value of 100% factor VIII activity, range 55-200%. The coefficients of variation of this unique technic, determined both by clotting time in sec (1.4%) and by the derived percentage activity (7.0%), reflect the greater reproducibility and reliability of the assay as compared with previously reported methods. We feel that this method of factor VIII assay should be utilized for specific diagnostic information regarding patients who have factor VIII disorders.", "contents": "A precision study of a photo-optical factor VIII assay technic. Various methods of factor VIII analysis with a wide range of accuracies as confirmed by calculated coefficients of variation have been devised. The authors report a technic of factor VIII assay using a photo-optical clot-detection instrument with commercially available sources of factor VIII-deficient plasma and lyophilized normal control plasma, and compare its accuracy, ease of performance and reliability with those of existing methods. The accuracy of the technic was established by a survey of a normal control group, which revealed a mean value of 100% factor VIII activity, range 55-200%. The coefficients of variation of this unique technic, determined both by clotting time in sec (1.4%) and by the derived percentage activity (7.0%), reflect the greater reproducibility and reliability of the assay as compared with previously reported methods. We feel that this method of factor VIII assay should be utilized for specific diagnostic information regarding patients who have factor VIII disorders."}
{"id": "PMID:474496", "title": "Analyzing large numbers of data with a programmable calculator. Technic and applications.", "content": "Programmable calculators or minicomputers can be used in many laboratory activities, including tasks for which large numbers of data must be analyzed. A technic for organizing data into records and files for storage on magnetic tape and for using an index to find the locations of stored data quickly is described. Applications of the technic for analysis of quality control results, determination of potential HLA-compatible tissue donors, and analysis of laboratory administrative data are presented. These applications were written for use with a small programmable calculator.", "contents": "Analyzing large numbers of data with a programmable calculator. Technic and applications. Programmable calculators or minicomputers can be used in many laboratory activities, including tasks for which large numbers of data must be analyzed. A technic for organizing data into records and files for storage on magnetic tape and for using an index to find the locations of stored data quickly is described. Applications of the technic for analysis of quality control results, determination of potential HLA-compatible tissue donors, and analysis of laboratory administrative data are presented. These applications were written for use with a small programmable calculator."}
{"id": "PMID:474497", "title": "Comparison of the errors due to the use of gauze and the use of filter paper in the gravimetric chloride titration sweat test.", "content": "Potential sources of error in the Gibson and Cooke method of sweat testing using gauze and filter paper as collection media as described. The absolute chloride content of gauze was higher than that of filter paper; however, the variability of chloride contents between the two was insignificant. Complete chloride elution from filter paper requires more time than elution from gauze. A method of determining probability of a clinically acceptable measurement based on sweat sample size, chloride determination, and variability of chloride in collection media is also described.", "contents": "Comparison of the errors due to the use of gauze and the use of filter paper in the gravimetric chloride titration sweat test. Potential sources of error in the Gibson and Cooke method of sweat testing using gauze and filter paper as collection media as described. The absolute chloride content of gauze was higher than that of filter paper; however, the variability of chloride contents between the two was insignificant. Complete chloride elution from filter paper requires more time than elution from gauze. A method of determining probability of a clinically acceptable measurement based on sweat sample size, chloride determination, and variability of chloride in collection media is also described."}
{"id": "PMID:474498", "title": "Diagnosis of toxoplasmosis by electron microscopic fine-structural analysis.", "content": "The analysis of fine structures by electron microscopic examination of ultrathin tissue sections permitted a diagnosis of toxoplasmosis in a fatal human case that would have gone undiagnosed by conventional methods. Examination of histologically prepared sections revealed organisms that were morphologically nondiagnostic. Fine-structural analysis showed the organisms to be 3 x 2 micrometer in size, to contain few micronemes, to contain as many as nine rhoptries, to possess an apical conoid, and to multiply by endodyogeny. The features corresponded to those observed in fine-structural analyses of Toxoplasma gondii grown in animals by Scholtyseck and Mehlhorn. Fine-structural analysis can be a valuable adjunct in the diagnosis of toxoplasmosis.", "contents": "Diagnosis of toxoplasmosis by electron microscopic fine-structural analysis. The analysis of fine structures by electron microscopic examination of ultrathin tissue sections permitted a diagnosis of toxoplasmosis in a fatal human case that would have gone undiagnosed by conventional methods. Examination of histologically prepared sections revealed organisms that were morphologically nondiagnostic. Fine-structural analysis showed the organisms to be 3 x 2 micrometer in size, to contain few micronemes, to contain as many as nine rhoptries, to possess an apical conoid, and to multiply by endodyogeny. The features corresponded to those observed in fine-structural analyses of Toxoplasma gondii grown in animals by Scholtyseck and Mehlhorn. Fine-structural analysis can be a valuable adjunct in the diagnosis of toxoplasmosis."}
{"id": "PMID:474499", "title": "Infection of a burn wound by Aspergillus niger. Gross appearance simulating ecthyma gangrenosa.", "content": "Growth of Aspergillus niger on a burn wound clinically simulated the early (hemorrhagic) phase of an invasive infection by Pseudomonas aeruginosa or ecthyma gangrenosa. Wound biopsy for histologic examination and culture readily yielded a definitive diagnosis of noninvasive mycotic infection of the burn wound. Pigmentation surrounding mycotic hyphae (otherwise typical for Aspergillus spp.) strongly suggested Aspergillus niger. Cultural data confirmed the diagnosis.", "contents": "Infection of a burn wound by Aspergillus niger. Gross appearance simulating ecthyma gangrenosa. Growth of Aspergillus niger on a burn wound clinically simulated the early (hemorrhagic) phase of an invasive infection by Pseudomonas aeruginosa or ecthyma gangrenosa. Wound biopsy for histologic examination and culture readily yielded a definitive diagnosis of noninvasive mycotic infection of the burn wound. Pigmentation surrounding mycotic hyphae (otherwise typical for Aspergillus spp.) strongly suggested Aspergillus niger. Cultural data confirmed the diagnosis."}
{"id": "PMID:474502", "title": "Accuracy in interlaboratory quality control programs.", "content": "Interlaboratory quality control programs provide an important contribution to achieving and maintaining accuracy in clinical laboratories. The various strategies for providing accurate target values in the two principal types of interlaboratory programs are reviewed.", "contents": "Accuracy in interlaboratory quality control programs. Interlaboratory quality control programs provide an important contribution to achieving and maintaining accuracy in clinical laboratories. The various strategies for providing accurate target values in the two principal types of interlaboratory programs are reviewed."}
{"id": "PMID:474503", "title": "Analytic clinical laboratory precision. State of the art for twenty-nine analytes.", "content": "Relationships of concentration and coefficient of variation are described for 29 clinical laboratory analytes. Estimated mean regression curves and the standard deviations of individual laboratory coefficients of variation about the mean regression are calculated. Twenty-seven analytes showed a significant relation between concentration and coefficient of variation. State of the art precision is compared to medical goals. The average coefficient of variation for one analyte, calcium, fails to meet medical goals for manual methods. The distribution of individual laboratory precision above average state of the art figures is discussed. The proportion of laboratories failing to meet medical goals is large for osmolality, as well as manual calcium methods.", "contents": "Analytic clinical laboratory precision. State of the art for twenty-nine analytes. Relationships of concentration and coefficient of variation are described for 29 clinical laboratory analytes. Estimated mean regression curves and the standard deviations of individual laboratory coefficients of variation about the mean regression are calculated. Twenty-seven analytes showed a significant relation between concentration and coefficient of variation. State of the art precision is compared to medical goals. The average coefficient of variation for one analyte, calcium, fails to meet medical goals for manual methods. The distribution of individual laboratory precision above average state of the art figures is discussed. The proportion of laboratories failing to meet medical goals is large for osmolality, as well as manual calcium methods."}
{"id": "PMID:474504", "title": "Stability of mean values of organic analytes in lyophilized quality control serum. A study utilizing data from the Quality Assurance Service (QAS) Program of the College of American Pathologists.", "content": "The long-term stability of mean values of six organic analytes in 48 commercial pools of lyophilized chemistry quality control serum is evaluated. These pools, provided by five manufacturers, have been used by nine Regional Quality Control Programs participating in the College of American Pathologists Quality Assurance Service. Creatinine yielded stable mean values in 88% of the pools studied. Both albumin and urea nitrogen demonstrated manufacturer and method-related changes in mean values. Bilirubin, cholesterol, and uric acid changes in mean values were independent of control serum manufacturer, predominant in calibrator-standardized automated procedures, and were clustered by years.", "contents": "Stability of mean values of organic analytes in lyophilized quality control serum. A study utilizing data from the Quality Assurance Service (QAS) Program of the College of American Pathologists. The long-term stability of mean values of six organic analytes in 48 commercial pools of lyophilized chemistry quality control serum is evaluated. These pools, provided by five manufacturers, have been used by nine Regional Quality Control Programs participating in the College of American Pathologists Quality Assurance Service. Creatinine yielded stable mean values in 88% of the pools studied. Both albumin and urea nitrogen demonstrated manufacturer and method-related changes in mean values. Bilirubin, cholesterol, and uric acid changes in mean values were independent of control serum manufacturer, predominant in calibrator-standardized automated procedures, and were clustered by years."}
{"id": "PMID:474505", "title": "The 1978 College of American Pathologists survey of analyses of five serum enzymes by 450 laboratories.", "content": "Sets of survey specimens having known linear interralationships were analyzed on four occasions by approximately 450 laboratories for the five enzymes lactate dehydrogenase, aspartate aminotransferase, creatine kinase, alanine aminotransferase, and alkaline phosphatase. The results are summarized in terms of the apparent precision and relative accuracy of various analytical systems, and some apparent problems in enzyme assays are identified. The results show that interlaboratory differences in enzyme analyses are not due primarily to differences in the way laboratorians utilize their analytical systems but rather are due to fundamental differences in the instruments and reagents supplied to the laboratorians. The attainment of interlaboratory comparability of enzyme analyses is a problem that can best be addressed by the manufacturers of instruments and reagents, rather than by individual laboratorians.", "contents": "The 1978 College of American Pathologists survey of analyses of five serum enzymes by 450 laboratories. Sets of survey specimens having known linear interralationships were analyzed on four occasions by approximately 450 laboratories for the five enzymes lactate dehydrogenase, aspartate aminotransferase, creatine kinase, alanine aminotransferase, and alkaline phosphatase. The results are summarized in terms of the apparent precision and relative accuracy of various analytical systems, and some apparent problems in enzyme assays are identified. The results show that interlaboratory differences in enzyme analyses are not due primarily to differences in the way laboratorians utilize their analytical systems but rather are due to fundamental differences in the instruments and reagents supplied to the laboratorians. The attainment of interlaboratory comparability of enzyme analyses is a problem that can best be addressed by the manufacturers of instruments and reagents, rather than by individual laboratorians."}
{"id": "PMID:474506", "title": "Evolution of the Urinary Chemistry, Survey Program of the CAP.", "content": "Thirteen analytes were presented in the Urine Chemistry Survey Program during the year 1978. These included amylase, calcium, phosphorus, sodium, potassium, creatinine, osmolality, total protein, glucose, and urea nitrogen as regular constituents. Optional analytes included 17-ketosteroids, 17-ketogenic steroids, and vanillylmandelic acid. Certain samples elevated sodium, potassium, calcium, and phosphorus concentration to stimulate human disease states and to test consensus results in ranges heretofore not possible. Serious loss of precision performance was not noted although some methods are noticeably affected. Improvement in interlaboratory precision has sllowed as compared with previous years.", "contents": "Evolution of the Urinary Chemistry, Survey Program of the CAP. Thirteen analytes were presented in the Urine Chemistry Survey Program during the year 1978. These included amylase, calcium, phosphorus, sodium, potassium, creatinine, osmolality, total protein, glucose, and urea nitrogen as regular constituents. Optional analytes included 17-ketosteroids, 17-ketogenic steroids, and vanillylmandelic acid. Certain samples elevated sodium, potassium, calcium, and phosphorus concentration to stimulate human disease states and to test consensus results in ranges heretofore not possible. Serious loss of precision performance was not noted although some methods are noticeably affected. Improvement in interlaboratory precision has sllowed as compared with previous years."}
{"id": "PMID:474507", "title": "The 1978 College of American Pathologists Therapeutic Drug Monitoring Interlaboratory Survey Program.", "content": "In 1978 the College of American Pathologists introduced a Therapeutic Drug Monitoring Interlaborabory Survey Program. Each participant recieved six vials of lyophilized human serum on two occasions, approximately 13 weeks apart. The specimens contained various combinations of 12 drugs in subtherapeutic, therapeutic, and toxic concentrations. The drugs included in the specimens were phenytoin, phenobarbital, primidone, ethosuximide, carbamazepine, digoxin, procainamide, N-acetylprocainamide, quinidine, theophylline, lithium, and gentamicin. The weighed-in quantity of each drug was used as the target value for that drug. The specimens were analyzed by a variety of analytic methods. Statistics were calculated for all results regardless of the analytic method used and for each individual method. This report presents the data from the Program.", "contents": "The 1978 College of American Pathologists Therapeutic Drug Monitoring Interlaboratory Survey Program. In 1978 the College of American Pathologists introduced a Therapeutic Drug Monitoring Interlaborabory Survey Program. Each participant recieved six vials of lyophilized human serum on two occasions, approximately 13 weeks apart. The specimens contained various combinations of 12 drugs in subtherapeutic, therapeutic, and toxic concentrations. The drugs included in the specimens were phenytoin, phenobarbital, primidone, ethosuximide, carbamazepine, digoxin, procainamide, N-acetylprocainamide, quinidine, theophylline, lithium, and gentamicin. The weighed-in quantity of each drug was used as the target value for that drug. The specimens were analyzed by a variety of analytic methods. Statistics were calculated for all results regardless of the analytic method used and for each individual method. This report presents the data from the Program."}
{"id": "PMID:474508", "title": "Changes in level of precision of common ligand assays during a seven-year interval.", "content": "Nine commonly performed radioligand analyses were studied for periods of four to seven years in terms of change in level of precision. Consistent improvement in precision is generally observed, except in specific instances when pool characteristics are altered to study sensitivity and crossreactivity.", "contents": "Changes in level of precision of common ligand assays during a seven-year interval. Nine commonly performed radioligand analyses were studied for periods of four to seven years in terms of change in level of precision. Consistent improvement in precision is generally observed, except in specific instances when pool characteristics are altered to study sensitivity and crossreactivity."}
{"id": "PMID:474509", "title": "Clinical laboratory estimations of serum and urinary phosphate.", "content": "Data from the 1977 College of American Pathologists (CAP) Chemistry Survey Program have been analyzed in order to evaluate currently used laboratory methods for determination of serum and urinary phosphate concentrations. The methods may be divided into those based on (1) molybdate and a reductant, (2) molybdate without a reductant (UV), (3) dye-binding, and (4) enzymatic (UV) assays. The majority of CAP Survey participants use a molybdate and reductant technic. Procedures using other reductants such as ferrous sulfate, ascorbate, and p-methylaminophenolsulfate manifest a slight positive bias. Compared to an overall mean concentration index, the SMAC-UV method manifests a considerable negative bias.", "contents": "Clinical laboratory estimations of serum and urinary phosphate. Data from the 1977 College of American Pathologists (CAP) Chemistry Survey Program have been analyzed in order to evaluate currently used laboratory methods for determination of serum and urinary phosphate concentrations. The methods may be divided into those based on (1) molybdate and a reductant, (2) molybdate without a reductant (UV), (3) dye-binding, and (4) enzymatic (UV) assays. The majority of CAP Survey participants use a molybdate and reductant technic. Procedures using other reductants such as ferrous sulfate, ascorbate, and p-methylaminophenolsulfate manifest a slight positive bias. Compared to an overall mean concentration index, the SMAC-UV method manifests a considerable negative bias."}
{"id": "PMID:474510", "title": "Serum uric acid.", "content": "Data from the 1977 College of American Pathologists (CAP) Comprehensive Chemistry Survey and other programs have been analyzed in an interlaboratory comparison of the measurements of serum uric acid. The majority of clinical laboratories utilize some form of phosphotungstic acid (PTA) oxidation-reduction technic for the measurement of uric acid. Methods based on uricase are most often performed on some automated device. In this Survey, in which specimens containing minimal potential interfering compounds were used, there are no significant differences in the results obtained by phosphotungstic acid and uricase methods.", "contents": "Serum uric acid. Data from the 1977 College of American Pathologists (CAP) Comprehensive Chemistry Survey and other programs have been analyzed in an interlaboratory comparison of the measurements of serum uric acid. The majority of clinical laboratories utilize some form of phosphotungstic acid (PTA) oxidation-reduction technic for the measurement of uric acid. Methods based on uricase are most often performed on some automated device. In this Survey, in which specimens containing minimal potential interfering compounds were used, there are no significant differences in the results obtained by phosphotungstic acid and uricase methods."}
{"id": "PMID:474511", "title": "Glucose measurements. A 1977 CAP Survey analysis.", "content": "An anlysis of the 1977 College of American Pathologists (CAP) Comprehensive Chemistry Survey program for glucose measurements is presented. Based on average mean concentrations compared with all-method mean concentration, hexokinase methods are the most accurate. Glucose oxidase-oxygen rate methods demonstrate the best precision in interlaboratory comparison statistics. Neocuproine methods for glucose manifest unacceptable positive biases when compared with overall mean concentrations and other methods. Accuracy and precision indices for glucose measurements in urine are well below those obtained in serum. Again, redox methods, such as those based on neocuproine and ferricyanide, perform so poorly that they should be eliminated.", "contents": "Glucose measurements. A 1977 CAP Survey analysis. An anlysis of the 1977 College of American Pathologists (CAP) Comprehensive Chemistry Survey program for glucose measurements is presented. Based on average mean concentrations compared with all-method mean concentration, hexokinase methods are the most accurate. Glucose oxidase-oxygen rate methods demonstrate the best precision in interlaboratory comparison statistics. Neocuproine methods for glucose manifest unacceptable positive biases when compared with overall mean concentrations and other methods. Accuracy and precision indices for glucose measurements in urine are well below those obtained in serum. Again, redox methods, such as those based on neocuproine and ferricyanide, perform so poorly that they should be eliminated."}
{"id": "PMID:474512", "title": "Laboratory intercomparison of commerical digoxin kits.", "content": "Results of recovery, precision, and effect of a protein deficient matrix on commercial digoxin assay kits is reviewed for pools distributed in 1978 by the Basic Ligand Assay Survey Program of College of American Pathologists. Variability among kits is present in terms of all three areas studied. Nevertheless, commonly used methods by participants have recovery rates approaching 100%; coefficients of variation are less than 10% at therapeutic serum levels.", "contents": "Laboratory intercomparison of commerical digoxin kits. Results of recovery, precision, and effect of a protein deficient matrix on commercial digoxin assay kits is reviewed for pools distributed in 1978 by the Basic Ligand Assay Survey Program of College of American Pathologists. Variability among kits is present in terms of all three areas studied. Nevertheless, commonly used methods by participants have recovery rates approaching 100%; coefficients of variation are less than 10% at therapeutic serum levels."}
{"id": "PMID:474513", "title": "Cap survey results for factor VIII assays (1977-1978).", "content": "This paper combines the data from the 1976, 1977, and 1978 CAP Surveys concerning factor VIII methods. The different instrument-reagent combinations are ranked according to precision and sensitivity. The results of questionnaires on specific assay technic from 1977 and 1978 are analyzed. The number of laboratories performing factor VIII assays has increased from 1976 to 1978. There was a poor correlation between precision and sensitivity in most systems. For any given system, there was considerable variation in precision from a normal factor VIII level specimen to a low factor VIII specimen. Certain technics, such as the dilutions on normal plasma used to construct the standard activity curve, show considerable interlaboratory variation. The need for standardization of factor VIII assay methods is evident from this study.", "contents": "Cap survey results for factor VIII assays (1977-1978). This paper combines the data from the 1976, 1977, and 1978 CAP Surveys concerning factor VIII methods. The different instrument-reagent combinations are ranked according to precision and sensitivity. The results of questionnaires on specific assay technic from 1977 and 1978 are analyzed. The number of laboratories performing factor VIII assays has increased from 1976 to 1978. There was a poor correlation between precision and sensitivity in most systems. For any given system, there was considerable variation in precision from a normal factor VIII level specimen to a low factor VIII specimen. Certain technics, such as the dilutions on normal plasma used to construct the standard activity curve, show considerable interlaboratory variation. The need for standardization of factor VIII assay methods is evident from this study."}
{"id": "PMID:474514", "title": "The Comprehensive Blood Bank Survey Program of the College of American Pathologists--1977.", "content": "The 1977 CAP Comprehensive Blood Bank Survey was sent to 2487 laboratories in the United States and five foreign countries. The results show that ABO and Rh testing has a continued concurrence of greater than 94.8% between participating and referee laboratories, while antibody detection was 95.9% effective on the type problem normally encountered in a transfusion service. Cross match showed a concurrence rate of 97.3% or better. Although these testings are artificial and cannot be directly extrapolated into effectiveness of patient care, they can be compared with each other and show a standard of excellency in the laboratory which is a good or better than that found in previous studies. A series of ungraded specimens and questionnaires shows that most laboratories used a 51 degree C heat elution method for antibody elution, but slightly better results were obtained with the Rubin technic. A second study showed that the grading of a positive direct antiglobulin test was fairly reproducible throughout the country.", "contents": "The Comprehensive Blood Bank Survey Program of the College of American Pathologists--1977. The 1977 CAP Comprehensive Blood Bank Survey was sent to 2487 laboratories in the United States and five foreign countries. The results show that ABO and Rh testing has a continued concurrence of greater than 94.8% between participating and referee laboratories, while antibody detection was 95.9% effective on the type problem normally encountered in a transfusion service. Cross match showed a concurrence rate of 97.3% or better. Although these testings are artificial and cannot be directly extrapolated into effectiveness of patient care, they can be compared with each other and show a standard of excellency in the laboratory which is a good or better than that found in previous studies. A series of ungraded specimens and questionnaires shows that most laboratories used a 51 degree C heat elution method for antibody elution, but slightly better results were obtained with the Rubin technic. A second study showed that the grading of a positive direct antiglobulin test was fairly reproducible throughout the country."}
{"id": "PMID:474515", "title": "Quality control of agar diffusion susceptibility tests.", "content": "Since its beginning in 1974 over 180 laboratories have participated in the Microbiology Program of The College of American Pathologists Quality Assurance Service submitting a total of 2,372,000 individual antibiotic determinations on three quality control reference stains. Eighty-nine and five tenths percent of these determinations were obtained using the standard Bauer-Kirby method; 8.4% using the agar overlay modification of Barry and associates. Standard statistical analysis of data obtained using the Bauer-Kirby method have been reported for each antimicrobic/reference strain combination. Comparisons have been made between the QAS data and those data obtained in earlier collaborative studies which currently serve as precision and accuracy control limits. In many cases QAS data exceed the existing control limits.", "contents": "Quality control of agar diffusion susceptibility tests. Since its beginning in 1974 over 180 laboratories have participated in the Microbiology Program of The College of American Pathologists Quality Assurance Service submitting a total of 2,372,000 individual antibiotic determinations on three quality control reference stains. Eighty-nine and five tenths percent of these determinations were obtained using the standard Bauer-Kirby method; 8.4% using the agar overlay modification of Barry and associates. Standard statistical analysis of data obtained using the Bauer-Kirby method have been reported for each antimicrobic/reference strain combination. Comparisons have been made between the QAS data and those data obtained in earlier collaborative studies which currently serve as precision and accuracy control limits. In many cases QAS data exceed the existing control limits."}
{"id": "PMID:474516", "title": "Identification of fecal parasites in the Special Parasitology Survey of the College of American Pathologists.", "content": "Review of participant performance in the Special Parasitology Survey of the College of American Pathologists for the years 1973-1977 shows that laboratories are generally able to identify helminth eggs and larvae in formalin-fixed fecal material but have difficulty identifying protozoa in formalin-fixed fecal material and in permanently stained slides. Ability to identify some protozoa has improved during the period reviewed. Well-trained personnel and access to reference materials should lead to improved performance.", "contents": "Identification of fecal parasites in the Special Parasitology Survey of the College of American Pathologists. Review of participant performance in the Special Parasitology Survey of the College of American Pathologists for the years 1973-1977 shows that laboratories are generally able to identify helminth eggs and larvae in formalin-fixed fecal material but have difficulty identifying protozoa in formalin-fixed fecal material and in permanently stained slides. Ability to identify some protozoa has improved during the period reviewed. Well-trained personnel and access to reference materials should lead to improved performance."}
{"id": "PMID:474517", "title": "Statistical principles underlying analytic goal-setting in clinical chemistry.", "content": "The Survey programs of the College of American Pathologists (CAP) have assessed current levels of analytic variance in many biochemical measurements, and a number of clinical chemists have proposed analytic goals. The practical importance of further reductions in analytic variance depends on the specific use of the laboratory test. Three general areas of application are described: 1) surveying a population to detect disease, 2) determining whether a particular individual's level of a given analyte is above or below a predefined alarm point, 3) monitoring an individual over a period of time to detect trends. Within each of these different contests, statistical methods are proposed for judging the practical effect of improvements in current levels of analytic precision, taking into account recent estimates of biological variation within the average individual and between individuals. As might be expected, reductions in analytic variance have greatest impact in those applications where biological variance is minimal. Such reductions will generally have little effect on the efficiency of a population survey but may be extremely valuable in decision-making concerning a particular hospital patient.", "contents": "Statistical principles underlying analytic goal-setting in clinical chemistry. The Survey programs of the College of American Pathologists (CAP) have assessed current levels of analytic variance in many biochemical measurements, and a number of clinical chemists have proposed analytic goals. The practical importance of further reductions in analytic variance depends on the specific use of the laboratory test. Three general areas of application are described: 1) surveying a population to detect disease, 2) determining whether a particular individual's level of a given analyte is above or below a predefined alarm point, 3) monitoring an individual over a period of time to detect trends. Within each of these different contests, statistical methods are proposed for judging the practical effect of improvements in current levels of analytic precision, taking into account recent estimates of biological variation within the average individual and between individuals. As might be expected, reductions in analytic variance have greatest impact in those applications where biological variance is minimal. Such reductions will generally have little effect on the efficiency of a population survey but may be extremely valuable in decision-making concerning a particular hospital patient."}
{"id": "PMID:474518", "title": "Mammary carcinoma with osteoclast-like giant cells. A study of eight cases with follow-up data.", "content": "Eight cases of a rare, distinctive variant of infiltrating mammary carcinoma featuring benign multinucleated osteoclast-like giant cells are reported. The multinucleated osteoclast-like giant cells are reported. The multinucleated giant cells were associated with ductal carcinoma in five cases and with infiltrating lobular carcinoma in three cases. Although three patients had lymph nodal metastases in level one, none of the nodal metastases contained giant cells. From the limited follow-up data of this report, it seems likely that the prognosis for patients who have this type of adenocarcinoma is not especially favorable. The observation that the giant cells generally occurred in areas of prominent angiogenesis suggests that the angiogenesis may be induced by some chemical substance produced by the tumor cells. Biochemical and immunologic investigations may eventually provide an explanation for this unusual morphologic manifestation of host reaction to mammary carcinoma.", "contents": "Mammary carcinoma with osteoclast-like giant cells. A study of eight cases with follow-up data. Eight cases of a rare, distinctive variant of infiltrating mammary carcinoma featuring benign multinucleated osteoclast-like giant cells are reported. The multinucleated osteoclast-like giant cells are reported. The multinucleated giant cells were associated with ductal carcinoma in five cases and with infiltrating lobular carcinoma in three cases. Although three patients had lymph nodal metastases in level one, none of the nodal metastases contained giant cells. From the limited follow-up data of this report, it seems likely that the prognosis for patients who have this type of adenocarcinoma is not especially favorable. The observation that the giant cells generally occurred in areas of prominent angiogenesis suggests that the angiogenesis may be induced by some chemical substance produced by the tumor cells. Biochemical and immunologic investigations may eventually provide an explanation for this unusual morphologic manifestation of host reaction to mammary carcinoma."}
{"id": "PMID:474519", "title": "Prognostic factors in adrenal cortical tumors. A mathematical analysis of clinical and morphologic data.", "content": "Forty-one patients with adrenal cortical tumors were evaluated retrospectively for the presence of histologic and non-histologic features associated with malignant behavior. The patients who survived operation were followed at least five years. The association of these features with subsequent metastasis was examined by both parametric and nonparametric statistical methods. Twelve criteria were statistically significant in predicting subsequent metastasis. The most significant of these were: clinical evidence of weight loss, broad fibrous bands traversing the tumor, a diffuse growth pattern, vascular invasion, tumor cell necrosis, and tumor mass. The tendency toward metastasis for each tumor was expressed in indices that correlated well with diagnoses made by conventional histologic means, but were more accurate in predicting subsequent clinical behavior than nonquantitative methods of evaluation.", "contents": "Prognostic factors in adrenal cortical tumors. A mathematical analysis of clinical and morphologic data. Forty-one patients with adrenal cortical tumors were evaluated retrospectively for the presence of histologic and non-histologic features associated with malignant behavior. The patients who survived operation were followed at least five years. The association of these features with subsequent metastasis was examined by both parametric and nonparametric statistical methods. Twelve criteria were statistically significant in predicting subsequent metastasis. The most significant of these were: clinical evidence of weight loss, broad fibrous bands traversing the tumor, a diffuse growth pattern, vascular invasion, tumor cell necrosis, and tumor mass. The tendency toward metastasis for each tumor was expressed in indices that correlated well with diagnoses made by conventional histologic means, but were more accurate in predicting subsequent clinical behavior than nonquantitative methods of evaluation."}
{"id": "PMID:474520", "title": "Studies of end-stage kidneys. III. Glycogen deposition in interstitial cells of the renal medulla.", "content": "Examination of kidneys of ten patients with uremia and severe hypertension treated by chronic intermittent hemodialysis revealed a deposition of glycogen within interstitial cells of the renal medulla. This is unlike any described renal distribution of glycogen. Electron microscopic studies performed in one case demonstrated monoparticulate glycogen both diffuse in the interstitial cell cytoplasm and locally aggregated beside lipid droplets. The findings provide evidence for a metabolic abnormality of renal medullary interstitial cells in patients who have protracted uremia.", "contents": "Studies of end-stage kidneys. III. Glycogen deposition in interstitial cells of the renal medulla. Examination of kidneys of ten patients with uremia and severe hypertension treated by chronic intermittent hemodialysis revealed a deposition of glycogen within interstitial cells of the renal medulla. This is unlike any described renal distribution of glycogen. Electron microscopic studies performed in one case demonstrated monoparticulate glycogen both diffuse in the interstitial cell cytoplasm and locally aggregated beside lipid droplets. The findings provide evidence for a metabolic abnormality of renal medullary interstitial cells in patients who have protracted uremia."}
{"id": "PMID:474521", "title": "Detection of fibrin monomer. Comparison of the immune precipitate method with the serial-dilution protamine sulfate test and the ethanol gel test.", "content": "In a previous publication, a method for measuring fibrin monomer in plasma with the use of an immune precipitate of fibrinogen was described. The method was found to be more sensitive to unstabilized fibrin monomer than the serial-dilution protamine sulfate test, or the ethanol gel test, and detected fibrin in a mixture of the plasmin digestion products of fibrin. In the present study the sensitivity of the immune precipitate method for detecting specific plasmin digestion products of fibrin X, Y, D, and E, its sensitivity for stabilized fibrin monomer complexes, and its sensitivity for fibrin retaining B-peptide were measured and compared with the corresponding sensitivities of the serial-dilution protamine sulfate test and the ethanol gel test for detecting these same products. The immune precipitate method was found to be highly sensitive to stabilized fibrin monomer complexes and to fibrin retaining B-peptide; to be significantly less sensitive to the plasmin digestion fragments X, Y, and E; and to be insensitive to fragment D. The serial-dilution protamine sulfate test and the ethanol gel test were found to be insensitive to all of the plasmin digestion products of fibrin and less sensitive to the other fibrin complexes.", "contents": "Detection of fibrin monomer. Comparison of the immune precipitate method with the serial-dilution protamine sulfate test and the ethanol gel test. In a previous publication, a method for measuring fibrin monomer in plasma with the use of an immune precipitate of fibrinogen was described. The method was found to be more sensitive to unstabilized fibrin monomer than the serial-dilution protamine sulfate test, or the ethanol gel test, and detected fibrin in a mixture of the plasmin digestion products of fibrin. In the present study the sensitivity of the immune precipitate method for detecting specific plasmin digestion products of fibrin X, Y, D, and E, its sensitivity for stabilized fibrin monomer complexes, and its sensitivity for fibrin retaining B-peptide were measured and compared with the corresponding sensitivities of the serial-dilution protamine sulfate test and the ethanol gel test for detecting these same products. The immune precipitate method was found to be highly sensitive to stabilized fibrin monomer complexes and to fibrin retaining B-peptide; to be significantly less sensitive to the plasmin digestion fragments X, Y, and E; and to be insensitive to fragment D. The serial-dilution protamine sulfate test and the ethanol gel test were found to be insensitive to all of the plasmin digestion products of fibrin and less sensitive to the other fibrin complexes."}
{"id": "PMID:474522", "title": "Reliable estimation of hemoglobin A2 concentration by electrophoresis with densitometry.", "content": "Elevated hemoglobin A2 (Hb A2) levels can be identified conveniently by densitometry after electrophoresis on cellulose acetate strips. Because a recent report questioned the accuracy of this technic, the method was re-evaluated by paired comparison with microcolumn chromatography. Analysis of 100 patient specimens showed high correlation (r = +0.84), but an average Hb A2 concentration 0.7% higher by densitometry than by chromatography (P less than 0.001). With upper limits set at 4.5%, and 3.8%, respectively, results were divided into \"normal\" and \"high\" for each method. Concordant results were obtained in 97 of the 100 cases (82 normal, 15 high). Another densitometer of improved design was used for paired analysis of 50 additional specimens, 25 normal and 25 with beta-thalassemia trait. The two groups were well separated by both procedures, and Hb A2 levels were similar (r = +0.92, P greater than 0.6). This study demonstrates that it is possible, with carefully controlled technics and properly calibrated instruments, to use electrophoresis with densitometry as a reliable means of identifying abnormal Hb A2 levels.", "contents": "Reliable estimation of hemoglobin A2 concentration by electrophoresis with densitometry. Elevated hemoglobin A2 (Hb A2) levels can be identified conveniently by densitometry after electrophoresis on cellulose acetate strips. Because a recent report questioned the accuracy of this technic, the method was re-evaluated by paired comparison with microcolumn chromatography. Analysis of 100 patient specimens showed high correlation (r = +0.84), but an average Hb A2 concentration 0.7% higher by densitometry than by chromatography (P less than 0.001). With upper limits set at 4.5%, and 3.8%, respectively, results were divided into \"normal\" and \"high\" for each method. Concordant results were obtained in 97 of the 100 cases (82 normal, 15 high). Another densitometer of improved design was used for paired analysis of 50 additional specimens, 25 normal and 25 with beta-thalassemia trait. The two groups were well separated by both procedures, and Hb A2 levels were similar (r = +0.92, P greater than 0.6). This study demonstrates that it is possible, with carefully controlled technics and properly calibrated instruments, to use electrophoresis with densitometry as a reliable means of identifying abnormal Hb A2 levels."}
{"id": "PMID:474523", "title": "Quantitation of hemoglobin F. An interlaboratory study.", "content": "Samples of whole blood from four hematologically normal adults and from two individuals with increased fetal hemoglobin levels were shipped to laboratories participating in the 1976 and 1977 Center for Disease Control (CDC) hemoglobinopathy proficiency testing surveys. The data from these surveys were used to evaluate the interlaboratory variability of current methods used to quantitate hemoglobin F (Hb F). Results of Hb F quantitation obtained from more than 100 laboratories than voluntarily participated in the survey were compared with those obtained from 21 reference laboratories. Individual values for all samples varied greatly among laboratories and among methods. Results returned by most of the laboratories were outside two standard deviations of the reference laboratory mean and were not accurate enough to differentiate between a normal level and an increased, abnormal level.", "contents": "Quantitation of hemoglobin F. An interlaboratory study. Samples of whole blood from four hematologically normal adults and from two individuals with increased fetal hemoglobin levels were shipped to laboratories participating in the 1976 and 1977 Center for Disease Control (CDC) hemoglobinopathy proficiency testing surveys. The data from these surveys were used to evaluate the interlaboratory variability of current methods used to quantitate hemoglobin F (Hb F). Results of Hb F quantitation obtained from more than 100 laboratories than voluntarily participated in the survey were compared with those obtained from 21 reference laboratories. Individual values for all samples varied greatly among laboratories and among methods. Results returned by most of the laboratories were outside two standard deviations of the reference laboratory mean and were not accurate enough to differentiate between a normal level and an increased, abnormal level."}
{"id": "PMID:474524", "title": "Intralaboratory quality control of hematology. Comparison of two systems.", "content": "Two systems for quality control have been compared, viz., the whole-blood control preparation method and the algorithm method using the geometric moving average XB and a new estimator Y. The system involving whole-blood controls has the advantage of simplicity of operation, but the economic cost of commercial preparations is often high. The algorith system has the advantage that results of all the test samples are used in the calculation; to some extent, this provides a buffer against random variation. The number of count-outs in a given channel is related to the precision of the channel, which in turn is a function of the number of determinations and calculations required for that result. An error of around 1% is introduced into the result each time a calculation is performed. A successful quality control scheme should contain elements of both control preparation and algorithm methods.", "contents": "Intralaboratory quality control of hematology. Comparison of two systems. Two systems for quality control have been compared, viz., the whole-blood control preparation method and the algorithm method using the geometric moving average XB and a new estimator Y. The system involving whole-blood controls has the advantage of simplicity of operation, but the economic cost of commercial preparations is often high. The algorith system has the advantage that results of all the test samples are used in the calculation; to some extent, this provides a buffer against random variation. The number of count-outs in a given channel is related to the precision of the channel, which in turn is a function of the number of determinations and calculations required for that result. An error of around 1% is introduced into the result each time a calculation is performed. A successful quality control scheme should contain elements of both control preparation and algorithm methods."}
{"id": "PMID:474525", "title": "Quantitation of rheumatoid factor activity by nephelometry.", "content": "Rheumatoid factor activity can be quantitated by use of nephelometric assay system. The data presented show a good correlation between this new method and the older quantitative latex tube rheumatoid factor test. In addition, the nephelometric and latex tube testing of sera from normal, rheumatoid and non-rheumatoid patients yielded similar results, suggesting the two procedures share a similar immunologic specificity. The new nephelometric test for rheumatoid factor is simple to perform, endpoints are objectively determined, and test data are highly reproducible.", "contents": "Quantitation of rheumatoid factor activity by nephelometry. Rheumatoid factor activity can be quantitated by use of nephelometric assay system. The data presented show a good correlation between this new method and the older quantitative latex tube rheumatoid factor test. In addition, the nephelometric and latex tube testing of sera from normal, rheumatoid and non-rheumatoid patients yielded similar results, suggesting the two procedures share a similar immunologic specificity. The new nephelometric test for rheumatoid factor is simple to perform, endpoints are objectively determined, and test data are highly reproducible."}
{"id": "PMID:474526", "title": "Pseudospirochetes, a cause of erroneous diagnoses of leptospirosis.", "content": "Motile filaments of varying lengths and thicknesses were observed by darkfield microscopy in a blood culture of a specimen from a patient who had fever of unknown origin. Similar structures were also seen in cultures inoculated with donor blood from healthy controls. Since the movement and configuration of the structures were not characteristic for spirochetes, the morphologic features were further examined by electron microscopy. The filaments observed were identical in appearance to pseudospirochetes described more than 50 years ago. The authors' observations indicate that they are derived from erythrocytes. Because of possible confusion of these pseudospirochetes with living organisms, the diagnosis of leptospirosis by darkfield microscopy should be confirmed by cultural or serologic tests.", "contents": "Pseudospirochetes, a cause of erroneous diagnoses of leptospirosis. Motile filaments of varying lengths and thicknesses were observed by darkfield microscopy in a blood culture of a specimen from a patient who had fever of unknown origin. Similar structures were also seen in cultures inoculated with donor blood from healthy controls. Since the movement and configuration of the structures were not characteristic for spirochetes, the morphologic features were further examined by electron microscopy. The filaments observed were identical in appearance to pseudospirochetes described more than 50 years ago. The authors' observations indicate that they are derived from erythrocytes. Because of possible confusion of these pseudospirochetes with living organisms, the diagnosis of leptospirosis by darkfield microscopy should be confirmed by cultural or serologic tests."}
{"id": "PMID:474527", "title": "Inhibition by ascorbic acid (vitamin C) of chemical detection of blood in urine.", "content": "Review of results of 9,620 consecutive urinalyses revealed 892 urines that contained microscopic evidence of erythrocytes and 98 for which there were discrepancies between microscopic evidence of hematuria (greater than 20 erythrocytes/high-power field) and chemical detection of blood (0 or \"1+\" when results should be \"3+\"). Eleven specimens (from ten patients) showed negative results of chemical tests for blood and greater than 40 erythrocytes/high-power field (HPF). Nine of these patients were receiving ascorbic acid supplementation. In a random sample of 20 patients with greater than 40 erythrocytes/HPF and strongly positive tests for blood, none was receiving ascorbic acid, a significant difference by chi-square analysis. In a prospective study, low levels of ascorbic acid inhibited chemical detection of blood. At 25 mg/dl ascorbic acid, 10--20 erythrocytes/HPF could not be detected; at 35 mg/dl ascorbic acid, greater than 20 erythrocytes/HPF were undetectable. For quantitation of the low level at which ascorbic acid inhibits chemical detection of blood, fresh urine specimens should be analyzed. Ascorbic acid is oxidated in vitro to products that partially inhibit detection of blood yet do not assay as ascorbic acid.", "contents": "Inhibition by ascorbic acid (vitamin C) of chemical detection of blood in urine. Review of results of 9,620 consecutive urinalyses revealed 892 urines that contained microscopic evidence of erythrocytes and 98 for which there were discrepancies between microscopic evidence of hematuria (greater than 20 erythrocytes/high-power field) and chemical detection of blood (0 or \"1+\" when results should be \"3+\"). Eleven specimens (from ten patients) showed negative results of chemical tests for blood and greater than 40 erythrocytes/high-power field (HPF). Nine of these patients were receiving ascorbic acid supplementation. In a random sample of 20 patients with greater than 40 erythrocytes/HPF and strongly positive tests for blood, none was receiving ascorbic acid, a significant difference by chi-square analysis. In a prospective study, low levels of ascorbic acid inhibited chemical detection of blood. At 25 mg/dl ascorbic acid, 10--20 erythrocytes/HPF could not be detected; at 35 mg/dl ascorbic acid, greater than 20 erythrocytes/HPF were undetectable. For quantitation of the low level at which ascorbic acid inhibits chemical detection of blood, fresh urine specimens should be analyzed. Ascorbic acid is oxidated in vitro to products that partially inhibit detection of blood yet do not assay as ascorbic acid."}
{"id": "PMID:474528", "title": "Erythrocytic ecdysis. An unusual morphologic finding in a case of sickle cell anemia with intercurrent cold-agglutinin syndrome.", "content": "Examination of peripheral blood smears from a patient with sickle cell disease during crisis associated with an upper respiratory tract infection, pneumonia, a positive Coombs test, and an elevated cold agglutinin titer revealed unique abnormalities associated with her erythrocytes, which have not been reported previously. In addition to the usual changes in peripheral blood occurring during crisis in sickle cell anemia, large numbers of free, filamentous forms that appeared to be derived from erythrocytes were present on repeated Wright's-stained smears. Erythrocytes were markedly distorted and had single and multiple surface protrusions that appeared to be antecedents of the free filamentous forms. Scanning electron microscopy confirmed the marked changes in the erythrocytes and revealed membrane alterations not apparent by use of the light microscope. It is suggested that the membrane stripping observed in this case was due to cold agglutinin--complement-mediated surface injury superimposed on the known abnormalities of the membranes of sickle erythrocytes.", "contents": "Erythrocytic ecdysis. An unusual morphologic finding in a case of sickle cell anemia with intercurrent cold-agglutinin syndrome. Examination of peripheral blood smears from a patient with sickle cell disease during crisis associated with an upper respiratory tract infection, pneumonia, a positive Coombs test, and an elevated cold agglutinin titer revealed unique abnormalities associated with her erythrocytes, which have not been reported previously. In addition to the usual changes in peripheral blood occurring during crisis in sickle cell anemia, large numbers of free, filamentous forms that appeared to be derived from erythrocytes were present on repeated Wright's-stained smears. Erythrocytes were markedly distorted and had single and multiple surface protrusions that appeared to be antecedents of the free filamentous forms. Scanning electron microscopy confirmed the marked changes in the erythrocytes and revealed membrane alterations not apparent by use of the light microscope. It is suggested that the membrane stripping observed in this case was due to cold agglutinin--complement-mediated surface injury superimposed on the known abnormalities of the membranes of sickle erythrocytes."}
{"id": "PMID:474529", "title": "Tumor-simulating retrorectal heterotopia of bone marrow.", "content": "A retrorectal tumor-simulating mass of extramedullary hematopoiesis was discovered during work-up for uterine bleeding in a 40-year-old woman. It was excised in toto. The only hematologic abnormality at the time was iron deficiency, which was corrected. Six years later the patient is free of recurrence or any hematologic abnormality. It is suggested that this case represents heterotopic marrow arising either as a remnant of embryonic hematopoiesis or from activated primitive cells retaining the embryonic potentiality of hematopoiesis. The difficult diagnostic problems, pathogenesis and treatment are discussed, and the importance of recognizing the trilineage of hematopoiesis using Wright's-stained imprints of the mass is emphasized.", "contents": "Tumor-simulating retrorectal heterotopia of bone marrow. A retrorectal tumor-simulating mass of extramedullary hematopoiesis was discovered during work-up for uterine bleeding in a 40-year-old woman. It was excised in toto. The only hematologic abnormality at the time was iron deficiency, which was corrected. Six years later the patient is free of recurrence or any hematologic abnormality. It is suggested that this case represents heterotopic marrow arising either as a remnant of embryonic hematopoiesis or from activated primitive cells retaining the embryonic potentiality of hematopoiesis. The difficult diagnostic problems, pathogenesis and treatment are discussed, and the importance of recognizing the trilineage of hematopoiesis using Wright's-stained imprints of the mass is emphasized."}
{"id": "PMID:474530", "title": "Myxomatous degeneration of the mitral valve complicated by nonbacterial thrombotic endocarditis with systemic embolization.", "content": "Myxomatous degeneration of the mitral valve is a disease of unknown etiology that is associated with many ominous complications. A case in which non-bacterial thrombotic endocarditis, superimposed on a myxomatous mitral valve, resulted in systemic embolization to the brain, heart, and kidney is presented. The purpose of this report is to describe a serious and previously unreported complication of myxomatous degeneration of the mitral valve.", "contents": "Myxomatous degeneration of the mitral valve complicated by nonbacterial thrombotic endocarditis with systemic embolization. Myxomatous degeneration of the mitral valve is a disease of unknown etiology that is associated with many ominous complications. A case in which non-bacterial thrombotic endocarditis, superimposed on a myxomatous mitral valve, resulted in systemic embolization to the brain, heart, and kidney is presented. The purpose of this report is to describe a serious and previously unreported complication of myxomatous degeneration of the mitral valve."}
{"id": "PMID:474532", "title": "Issues in replicating the trauma--Stren conversion.", "content": "The Trauma--Stren (T-S) conversion procedure was examined at a large state university and the construct related to personality and cognitive process variables. Significantly more respondents reported T-S conversion than in the original studies. Conversion was not significantly correlated with personal variables. The presence of experiences in which an event was reported as simultaneously traumatic and strenful is reported, and the criteria for clearly identifying the conversion phenomenon are questioned.", "contents": "Issues in replicating the trauma--Stren conversion. The Trauma--Stren (T-S) conversion procedure was examined at a large state university and the construct related to personality and cognitive process variables. Significantly more respondents reported T-S conversion than in the original studies. Conversion was not significantly correlated with personal variables. The presence of experiences in which an event was reported as simultaneously traumatic and strenful is reported, and the criteria for clearly identifying the conversion phenomenon are questioned."}
{"id": "PMID:474533", "title": "Attitudinal versus ecological approaches to the characterization of institutional treatment environments.", "content": "The relational and discriminative properties of attitudinal and ecological measures of environments were examined within the context of a state hospital for mentally retarted clients. Three hypotheses were advanced, and the results gave substantial support for one of these and partial support for a second: (a) that the correlations between attitudinal and ecological measures should not differ significantly from zero, and (b) that differences among hospital treatment programs and living units should be reflected in ecological but not in attitudinal measures. However, the results did not provide clear confirmation for a third hypothesis: that differences among staff demographic characteristics and employment history should be reflected in attitudinal but not in ecological measures. It was suggested that these background variables may not be appropriate measures of personality-linked attributes.", "contents": "Attitudinal versus ecological approaches to the characterization of institutional treatment environments. The relational and discriminative properties of attitudinal and ecological measures of environments were examined within the context of a state hospital for mentally retarted clients. Three hypotheses were advanced, and the results gave substantial support for one of these and partial support for a second: (a) that the correlations between attitudinal and ecological measures should not differ significantly from zero, and (b) that differences among hospital treatment programs and living units should be reflected in ecological but not in attitudinal measures. However, the results did not provide clear confirmation for a third hypothesis: that differences among staff demographic characteristics and employment history should be reflected in attitudinal but not in ecological measures. It was suggested that these background variables may not be appropriate measures of personality-linked attributes."}
{"id": "PMID:474534", "title": "Relationships among experience level and value orientation and the performance of paraprofessional telephone counselors.", "content": "The effectiveness of indigenous paraprofessional helpers is thought to be partially due to the similarity in style and values between then and their clients. However, there is a concern that as they gain experience in an agency, their identification may shift from the clients to the agency. Also, there is a question as to how effective paraprofessionals (selected due to their shared values with a target population) are with value-dissimilar clients. Truax and Carkhuff's scales of facilitative conditions as well as a technique functioning scale were used to assess the helping behavior of paraprofessional telephone counselors at three levels of experience in response to value-similar and value-dissimilar simulated calls. The results indicate that performance generally improved with training and experience and that instead of a uniform deterioration with dissimilar value calls, a complex relationship existed between performance, values, and experience level.", "contents": "Relationships among experience level and value orientation and the performance of paraprofessional telephone counselors. The effectiveness of indigenous paraprofessional helpers is thought to be partially due to the similarity in style and values between then and their clients. However, there is a concern that as they gain experience in an agency, their identification may shift from the clients to the agency. Also, there is a question as to how effective paraprofessionals (selected due to their shared values with a target population) are with value-dissimilar clients. Truax and Carkhuff's scales of facilitative conditions as well as a technique functioning scale were used to assess the helping behavior of paraprofessional telephone counselors at three levels of experience in response to value-similar and value-dissimilar simulated calls. The results indicate that performance generally improved with training and experience and that instead of a uniform deterioration with dissimilar value calls, a complex relationship existed between performance, values, and experience level."}
{"id": "PMID:474535", "title": "Goal attainment and satisfaction scores for CMHC clients.", "content": "The purpose of this study was to explore the psychometric properties of goal attainment scales and client satisfaction measures. The findings revealed that goal attainment results, like other types of self-report psychotherapy outcome measures, are directly related to (a) indicators of a client's social class, and (b) the manner of treatment termination (unilateral dropout vs. mutual consent). The extent of client--therapist agreement on goal scores was relatively low thereby supporting the necessity of obtaining both perspectives in evaluating treatment outcome. Furthermore, because measures of client satisfaction were found to be sufficiently independent from goal outcome scores, it was concluded that they provide a unique source of program evaluation data. Finally, it was concluded that Goal Attainment Scaling is best considered to be a quality assurance tool for individual client/therapist dyads rather than a mechanism for the determination of the effectiveness of specific mental health programs and services.", "contents": "Goal attainment and satisfaction scores for CMHC clients. The purpose of this study was to explore the psychometric properties of goal attainment scales and client satisfaction measures. The findings revealed that goal attainment results, like other types of self-report psychotherapy outcome measures, are directly related to (a) indicators of a client's social class, and (b) the manner of treatment termination (unilateral dropout vs. mutual consent). The extent of client--therapist agreement on goal scores was relatively low thereby supporting the necessity of obtaining both perspectives in evaluating treatment outcome. Furthermore, because measures of client satisfaction were found to be sufficiently independent from goal outcome scores, it was concluded that they provide a unique source of program evaluation data. Finally, it was concluded that Goal Attainment Scaling is best considered to be a quality assurance tool for individual client/therapist dyads rather than a mechanism for the determination of the effectiveness of specific mental health programs and services."}
{"id": "PMID:474536", "title": "Maladapting preschool children: identification, diagnosis, and remediation.", "content": "This article evaluates a preschool intervention program conducted as a part of the Wayne State University Preschool Project. The project's aim is (a) to provide early identification of preschool age children experiencing mental health problems, and (b) to reverse the diagnosed deficiencies, particularly among high risk children. The program was evaluated with children ranging in age from 3 to 4 years, within demographically comparable regions in the Detroit School System. Pretest and posttest measures were taken on the AML Scale and the Caldwell Preschool Inventory. Pretest scores were used to establish high risk children. A prescriptive intervention program was established within one region, aimed primarily at reducing adjustment problems among high risk children. The analysis of posttest data revealed more favorable scores among experimental children when compared to control children. In addition, evidence is provided of specific benefits for high risk children in the experimental program.", "contents": "Maladapting preschool children: identification, diagnosis, and remediation. This article evaluates a preschool intervention program conducted as a part of the Wayne State University Preschool Project. The project's aim is (a) to provide early identification of preschool age children experiencing mental health problems, and (b) to reverse the diagnosed deficiencies, particularly among high risk children. The program was evaluated with children ranging in age from 3 to 4 years, within demographically comparable regions in the Detroit School System. Pretest and posttest measures were taken on the AML Scale and the Caldwell Preschool Inventory. Pretest scores were used to establish high risk children. A prescriptive intervention program was established within one region, aimed primarily at reducing adjustment problems among high risk children. The analysis of posttest data revealed more favorable scores among experimental children when compared to control children. In addition, evidence is provided of specific benefits for high risk children in the experimental program."}
{"id": "PMID:474537", "title": "Factors associated with number of treatment interviews of patients from a barrio neighborhood mental health service: a cross-validation.", "content": "Demographic and treatment variables were, for a second time, found associated with number of treatment interviews completed by lower socioeconomic patients of a barrio area neighborhood mental health service. Of the eight variables that originally differentiated patients with respect to number of interviews, six remained significant on cross-validation. The longer staying patients were characterized by: young adult age range; disrupted marital status; self-referral; using psychotropic medication; major and secondary problems of anxiety and depression.", "contents": "Factors associated with number of treatment interviews of patients from a barrio neighborhood mental health service: a cross-validation. Demographic and treatment variables were, for a second time, found associated with number of treatment interviews completed by lower socioeconomic patients of a barrio area neighborhood mental health service. Of the eight variables that originally differentiated patients with respect to number of interviews, six remained significant on cross-validation. The longer staying patients were characterized by: young adult age range; disrupted marital status; self-referral; using psychotropic medication; major and secondary problems of anxiety and depression."}
{"id": "PMID:474540", "title": "Parathyroid autotransplantation in renal osteodystrophy.", "content": "Severe renal osteodystrophy with metaphyseal fractures developed in two children with hypoplastic-dysplastic kidneys and chronic renal failure despite therapy with vitamin D, CaCO3, phosphate-binding agents, and protein restriction. Serum immunoreactive parathyroid hormone (iPTH) levels were elevated to 709 and 1,537 pg/mL (N = 255 +/- 92 pg/mL). Total parathyroidectomy and then autotransplantation of a small portion of parathyroids into the left brachioradialis muscle resulted in complete healing of renal osteodystrophy with the same dose of vitamin D. Serum iPTH and histological studies have demonstrated functioning parathyroid autotransplants, 19 and 20 months postoperatively in these two patients. Advantage of such a procedure over 3 3/4 parathyroidectomy is that this transplanted parathyroid tissue is easily accessible for partial removal in case of recurrence of uncontrollable hyperparathyroidism. We believe that total parathyroidectomy and autotransplantation can be successfully performed even in small children.", "contents": "Parathyroid autotransplantation in renal osteodystrophy. Severe renal osteodystrophy with metaphyseal fractures developed in two children with hypoplastic-dysplastic kidneys and chronic renal failure despite therapy with vitamin D, CaCO3, phosphate-binding agents, and protein restriction. Serum immunoreactive parathyroid hormone (iPTH) levels were elevated to 709 and 1,537 pg/mL (N = 255 +/- 92 pg/mL). Total parathyroidectomy and then autotransplantation of a small portion of parathyroids into the left brachioradialis muscle resulted in complete healing of renal osteodystrophy with the same dose of vitamin D. Serum iPTH and histological studies have demonstrated functioning parathyroid autotransplants, 19 and 20 months postoperatively in these two patients. Advantage of such a procedure over 3 3/4 parathyroidectomy is that this transplanted parathyroid tissue is easily accessible for partial removal in case of recurrence of uncontrollable hyperparathyroidism. We believe that total parathyroidectomy and autotransplantation can be successfully performed even in small children."}
{"id": "PMID:474541", "title": "The cutaneous manifestations of child abuse and neglect.", "content": "The most easily recognizable physical manifestations of child abuse and neglect are seen in the skin. The findings may be the result of intentional trauma, neglect to provide adequate child care, alterations in family dynamics, or the emotional problems secondary to the maltreatment.", "contents": "The cutaneous manifestations of child abuse and neglect. The most easily recognizable physical manifestations of child abuse and neglect are seen in the skin. The findings may be the result of intentional trauma, neglect to provide adequate child care, alterations in family dynamics, or the emotional problems secondary to the maltreatment."}
{"id": "PMID:474542", "title": "Burns as a manifestation of child abuse and neglect.", "content": "A multidisciplinary child abuse team investigated children with burns admitted to a general hospital in Tulsa, Okla. Of the 26 burn cases investigated, 14 were found to be accidental, seven accidental with extreme degrees of neglect, and five nonaccidental burns. There are correlations between physical findings and social characteristics, which may aid in the identification and disposition of children with burns. A multiplicity of injuries suggests that nonaccidental burns are not spontaneous abuse, but a planned form of aggression. The high mortality of the abused group indicates the seriousness and severity of nonaccidental injuries. This experience has also shown that nonaccidental burns in children demand a thorough investigation of medical, social, and emotional factors.", "contents": "Burns as a manifestation of child abuse and neglect. A multidisciplinary child abuse team investigated children with burns admitted to a general hospital in Tulsa, Okla. Of the 26 burn cases investigated, 14 were found to be accidental, seven accidental with extreme degrees of neglect, and five nonaccidental burns. There are correlations between physical findings and social characteristics, which may aid in the identification and disposition of children with burns. A multiplicity of injuries suggests that nonaccidental burns are not spontaneous abuse, but a planned form of aggression. The high mortality of the abused group indicates the seriousness and severity of nonaccidental injuries. This experience has also shown that nonaccidental burns in children demand a thorough investigation of medical, social, and emotional factors."}
{"id": "PMID:474543", "title": "Relative usefulness of three growth hormone stimulation screening tests.", "content": "One hundred ninety-one children were tested by one of three screening procedures for growth hormone deficiency over a five-year period. Sleep screen yielded a 31.3% false-positive rate; levodopa administration alone yielded a 20.5% false-positive rate and levodopa in combination with propranolol yielded a 5.2% false-positive rate. These results support the view that the combined levodopa-propranolol hydrochloride screen test is superior to either the sleep or the levodopa screen tests in limiting the number of false-positive results and thus the need for further, more extensive testing.", "contents": "Relative usefulness of three growth hormone stimulation screening tests. One hundred ninety-one children were tested by one of three screening procedures for growth hormone deficiency over a five-year period. Sleep screen yielded a 31.3% false-positive rate; levodopa administration alone yielded a 20.5% false-positive rate and levodopa in combination with propranolol yielded a 5.2% false-positive rate. These results support the view that the combined levodopa-propranolol hydrochloride screen test is superior to either the sleep or the levodopa screen tests in limiting the number of false-positive results and thus the need for further, more extensive testing."}
{"id": "PMID:474544", "title": "Postvaricella suppurative meningitis. Case reports and review of the literature.", "content": "Suppurative meningitis should be recognized as being a complication of varicella. The clinician must assiduously exclude suppurative meningitis that at times may be clinically indistinguishable from the typical postinfectious encephalomyelitis of varicella. Misdiagnosis of the cause of CNS alterations during the course of varicella is possible.", "contents": "Postvaricella suppurative meningitis. Case reports and review of the literature. Suppurative meningitis should be recognized as being a complication of varicella. The clinician must assiduously exclude suppurative meningitis that at times may be clinically indistinguishable from the typical postinfectious encephalomyelitis of varicella. Misdiagnosis of the cause of CNS alterations during the course of varicella is possible."}
{"id": "PMID:474545", "title": "Increased incidence of lactobezoars in low birth weight infants.", "content": "Fifteen low birth weight infants had their conditions complicated by the formation of a lactobezoar. The mean gestational age was 30.3 weeks; mean birth weight was 1,184 g, and the mean age at the time of diagnosis was 11.8 days. Twelve of the infants were receiving an 80 kcal/dL-formula and one infant, a 40 kcal/dL-formula designed for the premature infant. Symptoms included abdominal distension, emesis or increased gastric residual, diarrhea, hematest-positive stools, abdominal mass, and gastric perforation.", "contents": "Increased incidence of lactobezoars in low birth weight infants. Fifteen low birth weight infants had their conditions complicated by the formation of a lactobezoar. The mean gestational age was 30.3 weeks; mean birth weight was 1,184 g, and the mean age at the time of diagnosis was 11.8 days. Twelve of the infants were receiving an 80 kcal/dL-formula and one infant, a 40 kcal/dL-formula designed for the premature infant. Symptoms included abdominal distension, emesis or increased gastric residual, diarrhea, hematest-positive stools, abdominal mass, and gastric perforation."}
{"id": "PMID:474546", "title": "Intraventricular hemorrhage in the neonate born at term.", "content": "Four term infants were found by computerized cerebral tomography (CT) to have intraventricular hemorrhage. All were male infants who had experienced substantial intrapartum complications. One infant had subtle clinical signs at 2 days of age; the others had major neurologic findings including seizures and ophthalmoplegia that occurred at 1, 4, and 6 weeks of age, respectively. Three infants in whom the hemorrhages were indistinguishable by CT from those described in preterm infants now have arrested hydrocephalus and normal development. The fourth infant was found to have a large glioblastoma at the site of origin of his initial hemorrhage. These cases emphasize the need to consider this diagnosis in neonates born at term who have abnormal neurologic signs.", "contents": "Intraventricular hemorrhage in the neonate born at term. Four term infants were found by computerized cerebral tomography (CT) to have intraventricular hemorrhage. All were male infants who had experienced substantial intrapartum complications. One infant had subtle clinical signs at 2 days of age; the others had major neurologic findings including seizures and ophthalmoplegia that occurred at 1, 4, and 6 weeks of age, respectively. Three infants in whom the hemorrhages were indistinguishable by CT from those described in preterm infants now have arrested hydrocephalus and normal development. The fourth infant was found to have a large glioblastoma at the site of origin of his initial hemorrhage. These cases emphasize the need to consider this diagnosis in neonates born at term who have abnormal neurologic signs."}
{"id": "PMID:474547", "title": "Catheterization of the posterior tibial artery in the neonate.", "content": "Catherization of the posterior tibial artery provides an alternative to cannulation of other arteries. We successfully catheterized this artery 17 times in 15 neonates whose weights ranged from 800 to 3,000 g. The catheters were placed percutaneously 13 times and by cutdown four times, and they remained in place for as long as 12 days (mean, 96 hours). Compromise of distal circulation was found in only two babies, both of whom had had umbilical artery catheters removed because of cyanosis of the toes that had resolved itself prior to posterior tibial artery cannulation. Our patients experienced no short-term complications from this procedure.", "contents": "Catheterization of the posterior tibial artery in the neonate. Catherization of the posterior tibial artery provides an alternative to cannulation of other arteries. We successfully catheterized this artery 17 times in 15 neonates whose weights ranged from 800 to 3,000 g. The catheters were placed percutaneously 13 times and by cutdown four times, and they remained in place for as long as 12 days (mean, 96 hours). Compromise of distal circulation was found in only two babies, both of whom had had umbilical artery catheters removed because of cyanosis of the toes that had resolved itself prior to posterior tibial artery cannulation. Our patients experienced no short-term complications from this procedure."}
{"id": "PMID:474548", "title": "Visual loss in osteopetrosis.", "content": "We present the clinical and electrophysiological findings in three cases of osteopetrosis with visual loss. In all cases the findings on electroretinography suggested diffuse retinal degeneration, despite normal ophthalmoscopic appearance of the retinas. We suggest that retinal degeneration may be a common finding in osteopetrosis and a major cause of visual loss. Patients with osteopetrosis and poor vision should be examined using electrophysiological techniques to detect unsuspected retinal dysfunction.", "contents": "Visual loss in osteopetrosis. We present the clinical and electrophysiological findings in three cases of osteopetrosis with visual loss. In all cases the findings on electroretinography suggested diffuse retinal degeneration, despite normal ophthalmoscopic appearance of the retinas. We suggest that retinal degeneration may be a common finding in osteopetrosis and a major cause of visual loss. Patients with osteopetrosis and poor vision should be examined using electrophysiological techniques to detect unsuspected retinal dysfunction."}
{"id": "PMID:474555", "title": "Symptomatic duodenal duplication cyst in an adult demonstrated by endoscopic retrograde cholangiopancreatography. Case report and literature review.", "content": "A patient with a symptomatic duodenal duplication cyst demonstrated by endoscopic retrograde cholangiopancreatography (ERCP) is presented. This lesion usually does not communicate with duodenal lumen but should be added to the list of cystic duodenal lesions demonstrable by ERCP. ERCP preoperatively is helpful to the surgeon in isolating adjacent duct structures but cyst wall histology is mandatory for correct anatomic diagnosis as this lesion has often been confused with Type III choledochal cysts.", "contents": "Symptomatic duodenal duplication cyst in an adult demonstrated by endoscopic retrograde cholangiopancreatography. Case report and literature review. A patient with a symptomatic duodenal duplication cyst demonstrated by endoscopic retrograde cholangiopancreatography (ERCP) is presented. This lesion usually does not communicate with duodenal lumen but should be added to the list of cystic duodenal lesions demonstrable by ERCP. ERCP preoperatively is helpful to the surgeon in isolating adjacent duct structures but cyst wall histology is mandatory for correct anatomic diagnosis as this lesion has often been confused with Type III choledochal cysts."}
{"id": "PMID:474556", "title": "Gallstone obstruction of duodenal cap (Bouveret's syndrome) diagnosed by endoscopy.", "content": "A patient presenting with signs and symptoms of gastric outlet obstruction and acute oliguric renal failure underwent early endoscopic examination. A large gallstone was seen obstructing the duodenal cap. Bouveret's syndrome and the role of endoscopy in early diagnosis are discussed.", "contents": "Gallstone obstruction of duodenal cap (Bouveret's syndrome) diagnosed by endoscopy. A patient presenting with signs and symptoms of gastric outlet obstruction and acute oliguric renal failure underwent early endoscopic examination. A large gallstone was seen obstructing the duodenal cap. Bouveret's syndrome and the role of endoscopy in early diagnosis are discussed."}
{"id": "PMID:474557", "title": "Zollinger-Ellison syndrome unresponsive to cimetidine.", "content": "A patient with Zollinger-Ellison syndrome appeared initially to respond to cimetidine with a reduction in gastric acid secretion. Symptoms immediately improved but after three days recurred with increasing severity. Intravenous cimetidine had only a short-lived and partial inhibitory effect on the rate of acid production and because of continuing pain and progressive bleeding from his duodenal ulcer, total gastrectomy was performed. Evidence of the effect of atropine and of oral and intravenous cimetidine is presented. Despite recent optimism, cimetidine is not always adequate treatment for Zollinger-Ellison syndrome.", "contents": "Zollinger-Ellison syndrome unresponsive to cimetidine. A patient with Zollinger-Ellison syndrome appeared initially to respond to cimetidine with a reduction in gastric acid secretion. Symptoms immediately improved but after three days recurred with increasing severity. Intravenous cimetidine had only a short-lived and partial inhibitory effect on the rate of acid production and because of continuing pain and progressive bleeding from his duodenal ulcer, total gastrectomy was performed. Evidence of the effect of atropine and of oral and intravenous cimetidine is presented. Despite recent optimism, cimetidine is not always adequate treatment for Zollinger-Ellison syndrome."}
{"id": "PMID:474558", "title": "Huge lipomatous pseudohypertrophy of the pancreas.", "content": "A rare case of lipomatous pseudohypertrophy of the pancreas associated with multiple cysts in an adult is presented. Endoscopic retrograde pancreatography showed a long, willow-like main pancreatic duct. Resected body and tail of the pancreas weighed 1,200 gm.", "contents": "Huge lipomatous pseudohypertrophy of the pancreas. A rare case of lipomatous pseudohypertrophy of the pancreas associated with multiple cysts in an adult is presented. Endoscopic retrograde pancreatography showed a long, willow-like main pancreatic duct. Resected body and tail of the pancreas weighed 1,200 gm."}
{"id": "PMID:474559", "title": "Pancreatic pseudocyst involving the spleen.", "content": "A case of pseudocyst of the tail of the pancreas involving the spleen is presented. Diagnosis of splenic involvement by pseudocyst was made by angiography followed by immediate (elective) surgical intervention with distal pancreatectomy and splenectomy without pre- or postoperative complications.", "contents": "Pancreatic pseudocyst involving the spleen. A case of pseudocyst of the tail of the pancreas involving the spleen is presented. Diagnosis of splenic involvement by pseudocyst was made by angiography followed by immediate (elective) surgical intervention with distal pancreatectomy and splenectomy without pre- or postoperative complications."}
{"id": "PMID:474560", "title": "Fused epiploic appendages simulating carcinoma of the left colon.", "content": "A 60-year old male with a history of rectal bleeding was operated upon because of the finding of a filling defect on barium enema examination. At operation it was found that the defect was produced by the fusion of two epiploic appendages. Following release of the adhesion the colon regained its normal appearance and postoperative barium enema showed no pathological findings.", "contents": "Fused epiploic appendages simulating carcinoma of the left colon. A 60-year old male with a history of rectal bleeding was operated upon because of the finding of a filling defect on barium enema examination. At operation it was found that the defect was produced by the fusion of two epiploic appendages. Following release of the adhesion the colon regained its normal appearance and postoperative barium enema showed no pathological findings."}
{"id": "PMID:474561", "title": "The endoscopy corner: reversible ischemic colitis--correlation of colonoscopic and pathologic changes.", "content": "Ischemic colitis is a well described complication following distal aortic replacement. The diagnosis is typically made by correlating clinical signs such as the occurrence of abdominal pain and bloody diarrhea with supporting radiographic changes. In this report, the diagnosis of ischemic colitis was confirmed on the basis of colonoscopic and associated histopathological findings. Colonoscopy is a useful technic in establishing the diagnosis of ischemic colitis, especially in patients whose clinical condition makes barium enema not feasible.", "contents": "The endoscopy corner: reversible ischemic colitis--correlation of colonoscopic and pathologic changes. Ischemic colitis is a well described complication following distal aortic replacement. The diagnosis is typically made by correlating clinical signs such as the occurrence of abdominal pain and bloody diarrhea with supporting radiographic changes. In this report, the diagnosis of ischemic colitis was confirmed on the basis of colonoscopic and associated histopathological findings. Colonoscopy is a useful technic in establishing the diagnosis of ischemic colitis, especially in patients whose clinical condition makes barium enema not feasible."}
{"id": "PMID:474562", "title": "The radiology corner: two cases showing the radiographic appearance of Meckel's stones.", "content": "The authors describe two cases of Meckel's diverticulum containing radiopaque stones that were diagnosed peroperatively by plain abdominal films and barium small bowel studies. One case of this rare condition was very unusual in that it had extremely symmetric, laminar calcifications on many projections.", "contents": "The radiology corner: two cases showing the radiographic appearance of Meckel's stones. The authors describe two cases of Meckel's diverticulum containing radiopaque stones that were diagnosed peroperatively by plain abdominal films and barium small bowel studies. One case of this rare condition was very unusual in that it had extremely symmetric, laminar calcifications on many projections."}
{"id": "PMID:474563", "title": "Leukemia among Nebraska farmers: a death certificate study.", "content": "The risk of leukemia among farmers was studied using records of death certificates from Nebraska, 1957--1974. Comparison of occupation, as recorded on the death certificate, for 1084 leukemia deaths and 2168 deaths from other causes, matched for age at death, year of death, county of residence, race, and sex, revealed an elevated risk of leukemia among farmers (odds ratio = 1.25). The risk was greatest among farmers born after 1900 and dying before age 66 (odds ratio = 1.83). Stratification by county of residence showed a significantly elevated risk for farmers from heavy corn producing counties.", "contents": "Leukemia among Nebraska farmers: a death certificate study. The risk of leukemia among farmers was studied using records of death certificates from Nebraska, 1957--1974. Comparison of occupation, as recorded on the death certificate, for 1084 leukemia deaths and 2168 deaths from other causes, matched for age at death, year of death, county of residence, race, and sex, revealed an elevated risk of leukemia among farmers (odds ratio = 1.25). The risk was greatest among farmers born after 1900 and dying before age 66 (odds ratio = 1.83). Stratification by county of residence showed a significantly elevated risk for farmers from heavy corn producing counties."}
{"id": "PMID:474564", "title": "Racial patterns of heart rate in an employed adult population.", "content": "This study explored racial patterns of heart rate in a large population of employed Chicagoans: 39,665 adults screened in Chicago firms between late 1967 and early 1973. Of these, 30,876 were white or black adults age 18--64 with complete data, and not on drug therapy for hypertension. Mean heart rates of young black men and women were significantly lower than mean heart rates of young white men and women. This difference was not present for the age groups 35 and older. The difference was present in both smokers and non-smokers; in normotensives and in persons with elevated blood pressure; and could not be explained by differences in relative body weight. The difference persisted after simultaneous adjustment for differences in the mean values of systolic blood pressure, relative weight, serum cholesterol, post-loaded plasma glucose, and number of cigarettes smoked per day.", "contents": "Racial patterns of heart rate in an employed adult population. This study explored racial patterns of heart rate in a large population of employed Chicagoans: 39,665 adults screened in Chicago firms between late 1967 and early 1973. Of these, 30,876 were white or black adults age 18--64 with complete data, and not on drug therapy for hypertension. Mean heart rates of young black men and women were significantly lower than mean heart rates of young white men and women. This difference was not present for the age groups 35 and older. The difference was present in both smokers and non-smokers; in normotensives and in persons with elevated blood pressure; and could not be explained by differences in relative body weight. The difference persisted after simultaneous adjustment for differences in the mean values of systolic blood pressure, relative weight, serum cholesterol, post-loaded plasma glucose, and number of cigarettes smoked per day."}
{"id": "PMID:474565", "title": "An investigation of coronary heart disease in families. The Framingham offspring study.", "content": "The Framingham Heart Study (FHS) was started in 1948 as a prospective investigation of cardiovascular disease in a cohort of adult men and women. Continuous surveillance of this sample of 5209 subjects has been maintained through biennial physical examinations. In 1971 examinations were begun on the children of the FHS cohort. This study, called the Framingham Offspring Study (FOS), was undertaken to expand upon knowledge of cardiovascular disease, particularly in the area of familial clustering of the disease and its risk factors. This report reviews the sampling design of the FHS and describes the nature of the FOS sample. The FOS families appear to be of typical size and age structure for families with parents born in the late 19th or early 20th century. In addition, there is little evidence that coronary heart disease (CHD) experience and CHD risk factors differ in parents of those who volunteered for this study and the parents of those who did not volunteer.", "contents": "An investigation of coronary heart disease in families. The Framingham offspring study. The Framingham Heart Study (FHS) was started in 1948 as a prospective investigation of cardiovascular disease in a cohort of adult men and women. Continuous surveillance of this sample of 5209 subjects has been maintained through biennial physical examinations. In 1971 examinations were begun on the children of the FHS cohort. This study, called the Framingham Offspring Study (FOS), was undertaken to expand upon knowledge of cardiovascular disease, particularly in the area of familial clustering of the disease and its risk factors. This report reviews the sampling design of the FHS and describes the nature of the FOS sample. The FOS families appear to be of typical size and age structure for families with parents born in the late 19th or early 20th century. In addition, there is little evidence that coronary heart disease (CHD) experience and CHD risk factors differ in parents of those who volunteered for this study and the parents of those who did not volunteer."}
{"id": "PMID:474566", "title": "A comparison of blood pressure, total cholesterol and cigarette smoking in parents in 1950 and their children in 1970.", "content": "Blood pressure and serum cholesterol levels as well as cigarette smoking are compared for parents who were examined in the Framingham Heart Study between 1948 and 1950 and their offspring who were studied in the early 1970s in the Framingham Offspring Study. For ages 30-49 years there are modest but significant differences in both systolic and diastolic blood pressure when mothers are compared to daughters, with the average pressures in the daughters being lower. Blood pressure differences in fathers and sons are not as large, and they disappear when families which include hypertensives are eliminated from the analysis. Total cholesterol levels were found to be significantly lower in both daughters and sons, but the difference was not large after adjusting for changes in methodology. The proportion of cigarette smokers was found to be substantially lower in the sons in all age groups, and modest reductions in cigarette smoking were also noted in all but the oldest (age 45--49 years) group of daughters.", "contents": "A comparison of blood pressure, total cholesterol and cigarette smoking in parents in 1950 and their children in 1970. Blood pressure and serum cholesterol levels as well as cigarette smoking are compared for parents who were examined in the Framingham Heart Study between 1948 and 1950 and their offspring who were studied in the early 1970s in the Framingham Offspring Study. For ages 30-49 years there are modest but significant differences in both systolic and diastolic blood pressure when mothers are compared to daughters, with the average pressures in the daughters being lower. Blood pressure differences in fathers and sons are not as large, and they disappear when families which include hypertensives are eliminated from the analysis. Total cholesterol levels were found to be significantly lower in both daughters and sons, but the difference was not large after adjusting for changes in methodology. The proportion of cigarette smokers was found to be substantially lower in the sons in all age groups, and modest reductions in cigarette smoking were also noted in all but the oldest (age 45--49 years) group of daughters."}
{"id": "PMID:474568", "title": "Responses to human diploid cell rabies vaccine: neutralizing antibody responses of vaccinees receiving booster doses of human diploid cell rabies vaccine.", "content": "Rabies neutralizing antibody levels were determined before and after administration of a booster-dose of Wyeth rabies vaccine (WRV) in persons immunized earlier with either duck embryo vaccine (DEV) or with WRV. Virtually all those receiving an initial 3-dose regimen of WRV (0, 7 and 21--28 days) still had neutralizing antibody one year later, but there was a decline in titer from 10--50 IU per ml at 35 days to about 1--3 IU. Only one-half of those receiving DEV as the primary vaccine had even detectable antibody one year later. All volunteers responded anamnestically to a single WRV booster given 8--12 months after either primary vaccine. Those given WRV initially had much higher antibody levels than those given DEV, but after the WRV booster antibody levels in all vaccinees remained high, even one year later.", "contents": "Responses to human diploid cell rabies vaccine: neutralizing antibody responses of vaccinees receiving booster doses of human diploid cell rabies vaccine. Rabies neutralizing antibody levels were determined before and after administration of a booster-dose of Wyeth rabies vaccine (WRV) in persons immunized earlier with either duck embryo vaccine (DEV) or with WRV. Virtually all those receiving an initial 3-dose regimen of WRV (0, 7 and 21--28 days) still had neutralizing antibody one year later, but there was a decline in titer from 10--50 IU per ml at 35 days to about 1--3 IU. Only one-half of those receiving DEV as the primary vaccine had even detectable antibody one year later. All volunteers responded anamnestically to a single WRV booster given 8--12 months after either primary vaccine. Those given WRV initially had much higher antibody levels than those given DEV, but after the WRV booster antibody levels in all vaccinees remained high, even one year later."}
{"id": "PMID:474569", "title": "The use of correlation and regression in the analysis of family resemblance.", "content": "The use of correlation and regression as applied to the analysis of family resemblance is discussed. The application of these statistical techniques to family data is not routine because the number of siblings per family is in general variable. Topics dealt with include estimation of sib-sib (intraclass) correlation, parent-child (interclass) correlation and regression of child on parent. An example is given.", "contents": "The use of correlation and regression in the analysis of family resemblance. The use of correlation and regression as applied to the analysis of family resemblance is discussed. The application of these statistical techniques to family data is not routine because the number of siblings per family is in general variable. Topics dealt with include estimation of sib-sib (intraclass) correlation, parent-child (interclass) correlation and regression of child on parent. An example is given."}
{"id": "PMID:474570", "title": "Principal component analysis of four health indicators and construction of a global health index in the aged.", "content": "The health status of 1025 urban senior citizens in a Montreal metropolitan area was determined by a questionnaire based on the subjects' view of physical, mental, and general well-being, and of dependence (indicators of Belloc et al., Berkman, Grogono and Woodgate, and Linn). A principal component analysis was made of these health indicators and two factors were retained. The first factor, which can be called a global health index, was used to compare the sexes and the age strata; it was thus found that men (those who survive) are generally healthier than the women, and that subjects from the lower-age strata are healthier than their older counterparts. The second factor is described as opposing physical health and dependence to mental health, the results of which were similar for both sexes, bur differed according to the various age strata compared.", "contents": "Principal component analysis of four health indicators and construction of a global health index in the aged. The health status of 1025 urban senior citizens in a Montreal metropolitan area was determined by a questionnaire based on the subjects' view of physical, mental, and general well-being, and of dependence (indicators of Belloc et al., Berkman, Grogono and Woodgate, and Linn). A principal component analysis was made of these health indicators and two factors were retained. The first factor, which can be called a global health index, was used to compare the sexes and the age strata; it was thus found that men (those who survive) are generally healthier than the women, and that subjects from the lower-age strata are healthier than their older counterparts. The second factor is described as opposing physical health and dependence to mental health, the results of which were similar for both sexes, bur differed according to the various age strata compared."}
{"id": "PMID:474571", "title": "Sickle cell anemia and trait in southern India: further studies.", "content": "Population surveys and family studies among 568 members of nine ethnic groups in southern India identified 15 homozygotes for sickle hemoglobin (HbS)who had mild clinical and hematological manifestations with high levels of fetal hemoglobin (mean=20%, range 8-36%) in a heterogeneous red cell distribution. In one family, the heterozygous mother had a hemoglobin pattern consistent with a form of the heterocellular hereditary persistence of fetal hemoglobin. Sickle cell trait was found in 153(27%) of those studied. Chromatographic quantitation of the hemoglobin fractions in these heterozygotes showed a trimodal distribution of the proportion of HB Sexplicable by a genetic model postulating the presence of genotypes with two (-alpha/-alpha), three (-alpha/alpha alpha) and four (alpha alpha/alpha alpha) active alpha-globin genes. Globin synthesis studies in four heterozygotes believed to have two active alpha-globin genes demonstrated an alpha/non-alpha total activity ratio (0.57) consistent with this model.", "contents": "Sickle cell anemia and trait in southern India: further studies. Population surveys and family studies among 568 members of nine ethnic groups in southern India identified 15 homozygotes for sickle hemoglobin (HbS)who had mild clinical and hematological manifestations with high levels of fetal hemoglobin (mean=20%, range 8-36%) in a heterogeneous red cell distribution. In one family, the heterozygous mother had a hemoglobin pattern consistent with a form of the heterocellular hereditary persistence of fetal hemoglobin. Sickle cell trait was found in 153(27%) of those studied. Chromatographic quantitation of the hemoglobin fractions in these heterozygotes showed a trimodal distribution of the proportion of HB Sexplicable by a genetic model postulating the presence of genotypes with two (-alpha/-alpha), three (-alpha/alpha alpha) and four (alpha alpha/alpha alpha) active alpha-globin genes. Globin synthesis studies in four heterozygotes believed to have two active alpha-globin genes demonstrated an alpha/non-alpha total activity ratio (0.57) consistent with this model."}
{"id": "PMID:474572", "title": "Heparin-induced thrombocytopenia: association with a platelet aggregating factor and arterial thromboses.", "content": "Eleven patients with heparin-induced thrombocytopenia were studied. Thrombocytopenia appeared 3-16 days following the initiation of prophylactic or therapeutic doses of heparin. The mean lowest platelet count recorded was 48,000/mm3. When heparin was stopped, recovery from thrombocytopenia began within 24 hours and was complete by ten days. Two patients developed fatal thromboses, and two others had myocardial infarctions while thrombo-cytopenic. In the serum of seven patients, including three of the four with arterial thrombosis, a heparin-dependent platelet aggregating factor was present. The factor caused release of platelet 14C serotonin but did not lyse platelets. It was present in the globulin fraction of all positive sera, and in one serum studied it was isolated in the IgG/IgA immunoglobulin fraction. The factor was not present in 16 normal sera or in the sera of 15 nonthrombocytopenic patients receiving heparin. Our observations suggest that heparin-induced thrombocytopenia is common and that, in some patients it may be accompanied by severe arterial thrombosis. In vivo platelet aggregation is a possible explanation for the thrombocytopenia and the thrombosis in this disorder.", "contents": "Heparin-induced thrombocytopenia: association with a platelet aggregating factor and arterial thromboses. Eleven patients with heparin-induced thrombocytopenia were studied. Thrombocytopenia appeared 3-16 days following the initiation of prophylactic or therapeutic doses of heparin. The mean lowest platelet count recorded was 48,000/mm3. When heparin was stopped, recovery from thrombocytopenia began within 24 hours and was complete by ten days. Two patients developed fatal thromboses, and two others had myocardial infarctions while thrombo-cytopenic. In the serum of seven patients, including three of the four with arterial thrombosis, a heparin-dependent platelet aggregating factor was present. The factor caused release of platelet 14C serotonin but did not lyse platelets. It was present in the globulin fraction of all positive sera, and in one serum studied it was isolated in the IgG/IgA immunoglobulin fraction. The factor was not present in 16 normal sera or in the sera of 15 nonthrombocytopenic patients receiving heparin. Our observations suggest that heparin-induced thrombocytopenia is common and that, in some patients it may be accompanied by severe arterial thrombosis. In vivo platelet aggregation is a possible explanation for the thrombocytopenia and the thrombosis in this disorder."}
{"id": "PMID:474573", "title": "Marked hypodiploidy in a case of acute undifferentiated leukemia.", "content": "A-29-year-old white man with acute undifferentiated leukemia had bone marrow cells with predominantly 42 chromosomes including several rearrangements. Following a brief induced \"complete\" remission, during which normal chromosomes were observed, bone marrow cells with 42 chromosomes but a different karyotype than before remission were found. Evaluation of that karyotype indicates not only a reduced chromosome count but also a reduced quantity of chromosome material.", "contents": "Marked hypodiploidy in a case of acute undifferentiated leukemia. A-29-year-old white man with acute undifferentiated leukemia had bone marrow cells with predominantly 42 chromosomes including several rearrangements. Following a brief induced \"complete\" remission, during which normal chromosomes were observed, bone marrow cells with 42 chromosomes but a different karyotype than before remission were found. Evaluation of that karyotype indicates not only a reduced chromosome count but also a reduced quantity of chromosome material."}
{"id": "PMID:474585", "title": "Identification and differentiation of surgically correctable hypertension due to primary aldosteronism.", "content": "During a protocol study for the evaluation of patients with primary aldosteronism, a variety of diagnostic studies were employed in an attempt to identify patients with primary aldosteronism and to differentiate patients with adrenal adenoma from patients with idiopathic adrenal hyperplasia. In this study, we are able to demonstrate the utility of (1) absent postural increase in plasma aldosterone concentration, (2) adrenal scanning and (3) normalization of blood pressure with spironolactone therapy in identifying patients with primary aldosterone excess who have an adrenal adenoma, surgical removal of which results in eliminating their hypertension.", "contents": "Identification and differentiation of surgically correctable hypertension due to primary aldosteronism. During a protocol study for the evaluation of patients with primary aldosteronism, a variety of diagnostic studies were employed in an attempt to identify patients with primary aldosteronism and to differentiate patients with adrenal adenoma from patients with idiopathic adrenal hyperplasia. In this study, we are able to demonstrate the utility of (1) absent postural increase in plasma aldosterone concentration, (2) adrenal scanning and (3) normalization of blood pressure with spironolactone therapy in identifying patients with primary aldosterone excess who have an adrenal adenoma, surgical removal of which results in eliminating their hypertension."}
{"id": "PMID:474590", "title": "The significance of exercise-induced Q waves.", "content": "The significance of transient exercise-induced Q waves present during treadmill testing was prospectively evaluated and correlated with the findings at cardiac catheterization. Exercise-induced Q waves were present in 14 of 560 patients (2.5 per cent) undergoing treadmill exercise testing. Thirteen patients had Q waves in leads V1 through V3 and one patient had Q waves in leads II, III and aVF. Ten patients underwent cardiac catheterization; six did not have coronary artery disease as determined by angiography. Two patients had a documented anterior myocardial infarction and did not undergo cardiac catheterization. Exercise myocardial perfusion imaging was performed in 10 patients. Six patients without coronary artery disease had no evidence of exercise perfusion defects. In the four patients with coronary artery disease, three patients had an abnormal resting perfusion study without change with exercise and one patient had a new exercise myocardial perfusion defect. We conclude that exercise-induced transient Q waves are not diagnostic of underlying coronary disease. In patients without coronary artery disease the mechanism of exercise-induced Q waves is as yet unclear. In patients with coronary artery disease, the mechanism may also be undefined or secondary to myocardial ischemia.", "contents": "The significance of exercise-induced Q waves. The significance of transient exercise-induced Q waves present during treadmill testing was prospectively evaluated and correlated with the findings at cardiac catheterization. Exercise-induced Q waves were present in 14 of 560 patients (2.5 per cent) undergoing treadmill exercise testing. Thirteen patients had Q waves in leads V1 through V3 and one patient had Q waves in leads II, III and aVF. Ten patients underwent cardiac catheterization; six did not have coronary artery disease as determined by angiography. Two patients had a documented anterior myocardial infarction and did not undergo cardiac catheterization. Exercise myocardial perfusion imaging was performed in 10 patients. Six patients without coronary artery disease had no evidence of exercise perfusion defects. In the four patients with coronary artery disease, three patients had an abnormal resting perfusion study without change with exercise and one patient had a new exercise myocardial perfusion defect. We conclude that exercise-induced transient Q waves are not diagnostic of underlying coronary disease. In patients without coronary artery disease the mechanism of exercise-induced Q waves is as yet unclear. In patients with coronary artery disease, the mechanism may also be undefined or secondary to myocardial ischemia."}
{"id": "PMID:474592", "title": "Cardiac abnormalities in myotonic dystrophy. Electrophysiologic and histopathologic studies.", "content": "Eight young adult male patients with myotonic dystrophy, mean age 26 years, underwent 24-hour Holter electrocardiographic monitoring and intracardiac electrophysiologic study. Right ventricular endomyocardial biopsies were performed at the end of the electrophysiologic study in five of them. The atrial to His[A-H] interval was 155 msec in one case and less than or equal to 55 msec in all patients. Twenty-four hour Holter electrocardiographic monitoring demonstrated more than 4 premature ventricular contractions per minute in two patients and marked cyclical sinus arrhythmia during sleep in two others. Electron microscopic analysis of the endomyocardial biopsy specimens disclosed no prominent sarcoplasmic reticulum abnormalities but prominent I bands compared to previously obtained controls. Myofibrillar degeneration was seen in all cases and was associated with abnormal mitochondria in two. Cardiac abnormalities can be detected very early in the evolution of myotonic dystrophy, even prior to the onset of cardiac symptoms. The reported abnormalities appear closely related to the pathologic process affecting other skeletal muscles.", "contents": "Cardiac abnormalities in myotonic dystrophy. Electrophysiologic and histopathologic studies. Eight young adult male patients with myotonic dystrophy, mean age 26 years, underwent 24-hour Holter electrocardiographic monitoring and intracardiac electrophysiologic study. Right ventricular endomyocardial biopsies were performed at the end of the electrophysiologic study in five of them. The atrial to His[A-H] interval was 155 msec in one case and less than or equal to 55 msec in all patients. Twenty-four hour Holter electrocardiographic monitoring demonstrated more than 4 premature ventricular contractions per minute in two patients and marked cyclical sinus arrhythmia during sleep in two others. Electron microscopic analysis of the endomyocardial biopsy specimens disclosed no prominent sarcoplasmic reticulum abnormalities but prominent I bands compared to previously obtained controls. Myofibrillar degeneration was seen in all cases and was associated with abnormal mitochondria in two. Cardiac abnormalities can be detected very early in the evolution of myotonic dystrophy, even prior to the onset of cardiac symptoms. The reported abnormalities appear closely related to the pathologic process affecting other skeletal muscles."}
{"id": "PMID:474596", "title": "Thrombocytosis, coronary thrombosis and acute myocardial infarction.", "content": "Clinical and morphologic findings are described in a 22 year old man with prolonged thromboyctosis, and coronary and splenic arterial thrombi causing myocardial and splenic infarcts. The absence of preexistent extensive coronary atherosclerosis, the presence of thrombus in more than one epicardial artery and in multiple intramural coronary arteries, the presence of arterial thrombosis in a noncoronary artery (splenic) and the absence of another apparent cause of the arterial thromboses are evidences that the intraarterial clotting in this patient was related to the severe thrombocytosis. A reveiw of the reported cases of vascular occlusion associated with thrombocytosis indicates that thrombi have infrequently been confirmed as the mechanism of the vascular occlusion. Although the frequency of vascular thrombi in patients with thrombocytosis has not been established, it is clear that vascular thrombosis can be a consequence of thrombocytosis and, as demonstrated by the present patient, that the coronary artery may be the site of the vascular occlusion, a heretofore unconfirmed event.", "contents": "Thrombocytosis, coronary thrombosis and acute myocardial infarction. Clinical and morphologic findings are described in a 22 year old man with prolonged thromboyctosis, and coronary and splenic arterial thrombi causing myocardial and splenic infarcts. The absence of preexistent extensive coronary atherosclerosis, the presence of thrombus in more than one epicardial artery and in multiple intramural coronary arteries, the presence of arterial thrombosis in a noncoronary artery (splenic) and the absence of another apparent cause of the arterial thromboses are evidences that the intraarterial clotting in this patient was related to the severe thrombocytosis. A reveiw of the reported cases of vascular occlusion associated with thrombocytosis indicates that thrombi have infrequently been confirmed as the mechanism of the vascular occlusion. Although the frequency of vascular thrombi in patients with thrombocytosis has not been established, it is clear that vascular thrombosis can be a consequence of thrombocytosis and, as demonstrated by the present patient, that the coronary artery may be the site of the vascular occlusion, a heretofore unconfirmed event."}
{"id": "PMID:474597", "title": "Intermittent Cushing's disease.", "content": "Described here is a young woman with intermittent Cushing's disease associated with psychotic disturbance. On five occasions, over a period of four years, she became severely depressed and had acne, amenorrhea, hirsuties and moon facies. On each of the first four occasions the symptoms lasted from two to three months and disappeared spontaneously. Biochemical investigations, including plasma ACTH estimations during the last two episodes, indicated that the adrenocortical overactivity was pituitary-dependent. During the fifth relapse the patient experienced a very severe, depressive psychosis, necessitating bilateral adrenalectomy, which resulted in complete remission.", "contents": "Intermittent Cushing's disease. Described here is a young woman with intermittent Cushing's disease associated with psychotic disturbance. On five occasions, over a period of four years, she became severely depressed and had acne, amenorrhea, hirsuties and moon facies. On each of the first four occasions the symptoms lasted from two to three months and disappeared spontaneously. Biochemical investigations, including plasma ACTH estimations during the last two episodes, indicated that the adrenocortical overactivity was pituitary-dependent. During the fifth relapse the patient experienced a very severe, depressive psychosis, necessitating bilateral adrenalectomy, which resulted in complete remission."}
{"id": "PMID:474598", "title": "Idiopathic, sustained, inappropriate secretion of ADH with associated hypertension and thirst.", "content": "A 15 year old girl presented with excessive thirst and hypertension (170/110 mm Hg). Biochemical investigations revealed serum sodium 118 meq/liter, serum osmolality 238 mosmol/liter, urine sodium 90 meq/liter, urine osmolality 700 mosmol/liter, persistenly elevated serum antidiuretic hormone (ADH) levels (5.8 to 11.9 pg/ml) and no obvious cause for the hypertension. The hypertension is, at least in part, volume-related, diminishing with fluid restriction. Features of gross water intoxication (e.g., confusion, coma) have not occurred. The etiology of the inappropriate secretion of ADH is not obvious but is not thought to be due to \"resetting of osmoreceptors\" as evidenced by failure to maximally dilute urine following a water load test and persistently elevated serum ADH levels. A similar patient described by Epstein and associates in 1962 is presently well with persistent features of inappropriate secretion of ADH.", "contents": "Idiopathic, sustained, inappropriate secretion of ADH with associated hypertension and thirst. A 15 year old girl presented with excessive thirst and hypertension (170/110 mm Hg). Biochemical investigations revealed serum sodium 118 meq/liter, serum osmolality 238 mosmol/liter, urine sodium 90 meq/liter, urine osmolality 700 mosmol/liter, persistenly elevated serum antidiuretic hormone (ADH) levels (5.8 to 11.9 pg/ml) and no obvious cause for the hypertension. The hypertension is, at least in part, volume-related, diminishing with fluid restriction. Features of gross water intoxication (e.g., confusion, coma) have not occurred. The etiology of the inappropriate secretion of ADH is not obvious but is not thought to be due to \"resetting of osmoreceptors\" as evidenced by failure to maximally dilute urine following a water load test and persistently elevated serum ADH levels. A similar patient described by Epstein and associates in 1962 is presently well with persistent features of inappropriate secretion of ADH."}
{"id": "PMID:474599", "title": "Concurrent hypersecretion of aldosterone and cortisol from the adrenal cortical adenoma.", "content": "Hypertension, hypokalemia, suppressed plasma renin activity and increased plasma aldosterone were found in a middle-aged woman. Following removal of the tumor in the left adrenal gland these abnormalities disappeared. Concurrently, however, the plasma cortisol level did not show normal diurnal change, although the value at 6 A.M. was within the normal range. Administration of 2 mg dexamethasone failed to depress the plasma cortisol level and urinary 17-OHCS concentrations. Postoperatively, plasma cortisol and urinary 17-OHCS were below normal. Histologic examination of the tumor indicated the presence of two types of adenoma cells; one was a large watery clear cell with rich lipid and possibly with aldosterone secretion and the other was an acidophilic cell with poor lipid and possibly with cortisol secretion. It is suggested that, in addition to oversecretion of aldosterone, the tumor autonomously secreted cortisol, although the amount of cortisol secreted was not large enough to produce typical Cushing's syndrome.", "contents": "Concurrent hypersecretion of aldosterone and cortisol from the adrenal cortical adenoma. Hypertension, hypokalemia, suppressed plasma renin activity and increased plasma aldosterone were found in a middle-aged woman. Following removal of the tumor in the left adrenal gland these abnormalities disappeared. Concurrently, however, the plasma cortisol level did not show normal diurnal change, although the value at 6 A.M. was within the normal range. Administration of 2 mg dexamethasone failed to depress the plasma cortisol level and urinary 17-OHCS concentrations. Postoperatively, plasma cortisol and urinary 17-OHCS were below normal. Histologic examination of the tumor indicated the presence of two types of adenoma cells; one was a large watery clear cell with rich lipid and possibly with aldosterone secretion and the other was an acidophilic cell with poor lipid and possibly with cortisol secretion. It is suggested that, in addition to oversecretion of aldosterone, the tumor autonomously secreted cortisol, although the amount of cortisol secreted was not large enough to produce typical Cushing's syndrome."}
{"id": "PMID:474600", "title": "Spontaneous remission of Cushing's disease. A case report and review of the literature.", "content": "A 70 year old man whose severe Cushing's disease remitted spontaneously is described. Medical therapy was instituted as a temporary measure prior to planned adrenalectomy. The patient showed dramatic improvement during medication and has remained well for two and a half years following the discontinuation of drug therapy. The literature is reviewed for other cases of spontaneous resolution of Cushing's.", "contents": "Spontaneous remission of Cushing's disease. A case report and review of the literature. A 70 year old man whose severe Cushing's disease remitted spontaneously is described. Medical therapy was instituted as a temporary measure prior to planned adrenalectomy. The patient showed dramatic improvement during medication and has remained well for two and a half years following the discontinuation of drug therapy. The literature is reviewed for other cases of spontaneous resolution of Cushing's."}
{"id": "PMID:474601", "title": "Hypertension and aldosterone overproduction without renin suppression in Cushing's syndrome from an adrenal adenoma.", "content": "Adrenal steroids and compenents of the renin-angiotensin system were measured before and after adrenalectomy in a woman with Cushing's syndrome and hypertension from a functioning adrenocortical adenoma. Aldosterone, deoxycorticosterone and cortisol were produced in excess by the adenoma, and were measured in tumor tissue. High plasma renin substrate concentrations, and normal basal and furosemide-stimulated plasma renin activities and plasma renin concentrations which were present before surgery, decreased after adrenalectomy, and the hypertension diminished. The inappropriately normal levels of renin and potassium in this patient, despite autonomous aldosterone overproduction, suggest an ineffective mineralocorticoid action of aldosterone, possibly from interaction with her other adenoma-produced steroids. The decrease in components of the renin-angiotensin system suggests a partial renin-dependence of her hypertension.", "contents": "Hypertension and aldosterone overproduction without renin suppression in Cushing's syndrome from an adrenal adenoma. Adrenal steroids and compenents of the renin-angiotensin system were measured before and after adrenalectomy in a woman with Cushing's syndrome and hypertension from a functioning adrenocortical adenoma. Aldosterone, deoxycorticosterone and cortisol were produced in excess by the adenoma, and were measured in tumor tissue. High plasma renin substrate concentrations, and normal basal and furosemide-stimulated plasma renin activities and plasma renin concentrations which were present before surgery, decreased after adrenalectomy, and the hypertension diminished. The inappropriately normal levels of renin and potassium in this patient, despite autonomous aldosterone overproduction, suggest an ineffective mineralocorticoid action of aldosterone, possibly from interaction with her other adenoma-produced steroids. The decrease in components of the renin-angiotensin system suggests a partial renin-dependence of her hypertension."}
{"id": "PMID:474602", "title": "Immunologically-mediated acute renal failure of nonglomerular origin in the course of systemic lupus erythematosus [SLE]. Report of two cases.", "content": "Acute anuric renal failure was observed in two patients with systemic lupus erythematosus (SLE) during the clinical and serologic active phase of the disease. Renal biopsies, performed during the acute episodes, showed only mild and focal mesangial cell proliferation without deposits. In contrast, tubulointerstitial lesions were predominant. Intense granular immune deposits along the tubular basement membrane, or immunofluorescence examination, were suggestive of immune complex deposition. One of these patients had severe high blood pressure and vascular lesions likely induced by immune complexes. In both, renal function was recovered. Immunologically-mediated tubular and vascular lesions in the course of SLE are discussed.", "contents": "Immunologically-mediated acute renal failure of nonglomerular origin in the course of systemic lupus erythematosus [SLE]. Report of two cases. Acute anuric renal failure was observed in two patients with systemic lupus erythematosus (SLE) during the clinical and serologic active phase of the disease. Renal biopsies, performed during the acute episodes, showed only mild and focal mesangial cell proliferation without deposits. In contrast, tubulointerstitial lesions were predominant. Intense granular immune deposits along the tubular basement membrane, or immunofluorescence examination, were suggestive of immune complex deposition. One of these patients had severe high blood pressure and vascular lesions likely induced by immune complexes. In both, renal function was recovered. Immunologically-mediated tubular and vascular lesions in the course of SLE are discussed."}
{"id": "PMID:474604", "title": "Immunodeficiency, malabsorption and secretory diarrhea. A new syndrome.", "content": "Described here is a patient with severe watery diarrhea associated with common variable immunodeficiency. Malabsorption for fat, bile acids, vitamin B12 and xylose was demonstrated, but the patient failed to respond to all the usual therapeutic maneuvers. The diarrhea responded only to high dose steroid therapy. Intestinal perfusion studies showed a hitherto undescribed, presumably acquired, glucose-stimulated water, sodium and chloride secretion in the jejunum and ileum, whereas normal fluid and electrolyte transport occurred from bicarbonate and mannitol solutions. Glucose absorption itself was normal and no hormonal, morphologic or biochemical defect was demonstrated to account for the phenomenon. The patient was also interesting when compared with other patients with common variable immunodeficiency in having normal plasma cells in the intestinal mucosa and an extensive family involvement.", "contents": "Immunodeficiency, malabsorption and secretory diarrhea. A new syndrome. Described here is a patient with severe watery diarrhea associated with common variable immunodeficiency. Malabsorption for fat, bile acids, vitamin B12 and xylose was demonstrated, but the patient failed to respond to all the usual therapeutic maneuvers. The diarrhea responded only to high dose steroid therapy. Intestinal perfusion studies showed a hitherto undescribed, presumably acquired, glucose-stimulated water, sodium and chloride secretion in the jejunum and ileum, whereas normal fluid and electrolyte transport occurred from bicarbonate and mannitol solutions. Glucose absorption itself was normal and no hormonal, morphologic or biochemical defect was demonstrated to account for the phenomenon. The patient was also interesting when compared with other patients with common variable immunodeficiency in having normal plasma cells in the intestinal mucosa and an extensive family involvement."}
{"id": "PMID:474606", "title": "Storage of microtiter plates for antimicrobial susceptibility tests.", "content": "Microdilution susceptibility tests are being performed routinely and in increasing numbers in many clinical laboratories. Microtiter plates for these assays are usually prepared in large batches and stored frozen until needed. Studies were performed to investigate the stability at -20C and at -70C of ampicillin, carbenicillin, ticarcillin, gentamicin, tobramycin, amikacin, chloramphenicol, and cephalothin, using Enterobacter aerogenes, Proteus mirabilis, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella as test organisms. The stability at -20C and -70C of clindamycin, vancomycin, cephalothin, cefazolin, oxacillin, methicillin, nafcillin, and penicillin was analyzed using Staphylococcus eureus, Staphylococcus epidermidis, Enterococcus, and alpha Streptococcus as the test organisms. No significant deterioration of any of the antibiotics was noted after ten weeks of storage at -70C, as detected by minimum inhibitory concentration determinations. No significant differences were noted at -20C for clindamycin, chloramphenicol, vancomycin, cefazolin, cephalothin, gentamicin, tobramycin, and amikacin. By ten weeks' storage at -20C, significant deterioration of carbenicillin, ticarcillin, ampicillin, penicillin, methicillin, oxacillin, and nafcillin was observed.", "contents": "Storage of microtiter plates for antimicrobial susceptibility tests. Microdilution susceptibility tests are being performed routinely and in increasing numbers in many clinical laboratories. Microtiter plates for these assays are usually prepared in large batches and stored frozen until needed. Studies were performed to investigate the stability at -20C and at -70C of ampicillin, carbenicillin, ticarcillin, gentamicin, tobramycin, amikacin, chloramphenicol, and cephalothin, using Enterobacter aerogenes, Proteus mirabilis, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella as test organisms. The stability at -20C and -70C of clindamycin, vancomycin, cephalothin, cefazolin, oxacillin, methicillin, nafcillin, and penicillin was analyzed using Staphylococcus eureus, Staphylococcus epidermidis, Enterococcus, and alpha Streptococcus as the test organisms. No significant deterioration of any of the antibiotics was noted after ten weeks of storage at -70C, as detected by minimum inhibitory concentration determinations. No significant differences were noted at -20C for clindamycin, chloramphenicol, vancomycin, cefazolin, cephalothin, gentamicin, tobramycin, and amikacin. By ten weeks' storage at -20C, significant deterioration of carbenicillin, ticarcillin, ampicillin, penicillin, methicillin, oxacillin, and nafcillin was observed."}
{"id": "PMID:474607", "title": "The use of examination content guidelines in teaching clinical microbiology.", "content": "The concept of using examination content guidelines as sources for curriculum content is presented, using the ASCP Board of Registry grids and a task list developed for HEW as a basis for proficiency examinations. The grid structure is related to Bloom's taxonomic levels, and a brief explanation of each level is included. A comparison of the grid structure and task list format is made, and specific examples of the use of each are offered.", "contents": "The use of examination content guidelines in teaching clinical microbiology. The concept of using examination content guidelines as sources for curriculum content is presented, using the ASCP Board of Registry grids and a task list developed for HEW as a basis for proficiency examinations. The grid structure is related to Bloom's taxonomic levels, and a brief explanation of each level is included. A comparison of the grid structure and task list format is made, and specific examples of the use of each are offered."}
{"id": "PMID:474608", "title": "A ladder curriculum in clinical microbiology.", "content": "A continuum of four microbiology courses for medical technology majors has been developed by the Medical Laboratory Science Program at Northeastern University. Using a system approach to curriculum design, the academic and clinical faculty identified career-entry capabilities, delineated appropriate subject content, and developed an instructional system which placed individual topics into one of four courses in the ladder curriculum: a basic, second-year, university-based clinical microbiology course stressing microbial technique and common organism identification; third-year microbiology and cellular physiology courses developing theoretical aspects; a fourth-year, hospital-based clinical microbiology rotation emphasizing isolation and identification techniques for significant pathogens; and a fourth-year, university-based, didactic course covering host defense-organism virulence interactions, infectious disease principles, and new techniques and unusual isolates as reported in the recent journal literature. Based on four years of experience with this system and in light of the publication of the 1978 American Society for Medical Technology (ASMT) Competency Statements, the Northeastern Medical Laboratory Science Program faculty is currently reexamining the continuum to insure completeness and appropriateness of overall subject content, to provide reinforcement, and to remove unnecessary duplication among the courses in the curriculum.", "contents": "A ladder curriculum in clinical microbiology. A continuum of four microbiology courses for medical technology majors has been developed by the Medical Laboratory Science Program at Northeastern University. Using a system approach to curriculum design, the academic and clinical faculty identified career-entry capabilities, delineated appropriate subject content, and developed an instructional system which placed individual topics into one of four courses in the ladder curriculum: a basic, second-year, university-based clinical microbiology course stressing microbial technique and common organism identification; third-year microbiology and cellular physiology courses developing theoretical aspects; a fourth-year, hospital-based clinical microbiology rotation emphasizing isolation and identification techniques for significant pathogens; and a fourth-year, university-based, didactic course covering host defense-organism virulence interactions, infectious disease principles, and new techniques and unusual isolates as reported in the recent journal literature. Based on four years of experience with this system and in light of the publication of the 1978 American Society for Medical Technology (ASMT) Competency Statements, the Northeastern Medical Laboratory Science Program faculty is currently reexamining the continuum to insure completeness and appropriateness of overall subject content, to provide reinforcement, and to remove unnecessary duplication among the courses in the curriculum."}
{"id": "PMID:474609", "title": "C3b receptors in normal human tissues.", "content": "Normal human kidney, lung, liver, heart, skin, thymus, spleen, lymph node, pancreas, and choroid plexus were reacted with a C3b-coated particle (fluoresceinated Salmonella typhi) to determine if these tissues contained C3b receptor cells. Clusters of these cells were identified in the spleen, lymph nodes, and in the renal glomeruli. All other studied tissues demonstrated a minimal homogeneous deposition of the indicator bacteria throughout the entire aspect of the tissue. Deposition of the indicator bacteria on the tissues was abrogated when the bacteria were prepared with heat inactivated serum as a source of complement.", "contents": "C3b receptors in normal human tissues. Normal human kidney, lung, liver, heart, skin, thymus, spleen, lymph node, pancreas, and choroid plexus were reacted with a C3b-coated particle (fluoresceinated Salmonella typhi) to determine if these tissues contained C3b receptor cells. Clusters of these cells were identified in the spleen, lymph nodes, and in the renal glomeruli. All other studied tissues demonstrated a minimal homogeneous deposition of the indicator bacteria throughout the entire aspect of the tissue. Deposition of the indicator bacteria on the tissues was abrogated when the bacteria were prepared with heat inactivated serum as a source of complement."}
{"id": "PMID:474611", "title": "A computer assisted test assembly system for medical technology examinations.", "content": "A descriptive report on the establishment and implementation of a computer assisted test assembly (CATA) system at the University of Iowa Medical Technology Program is presented. This system is an alternative approach to the production of paper and pencil examinations that may be considered for use in other medical technology programs. Access to the system is designed, through coding, to correlate with specific instructional objectives in a variety of subject areas. The CATA system has capabilities for mass production of examinations, correction of examinations, and production of accumulative item analyses. Use of this computerized system has produced savings in terms of money, faculty time, and clerical assistance. Primarily, however, it has served to effectively relate instructional objectives in an educational program to the evaluation process.", "contents": "A computer assisted test assembly system for medical technology examinations. A descriptive report on the establishment and implementation of a computer assisted test assembly (CATA) system at the University of Iowa Medical Technology Program is presented. This system is an alternative approach to the production of paper and pencil examinations that may be considered for use in other medical technology programs. Access to the system is designed, through coding, to correlate with specific instructional objectives in a variety of subject areas. The CATA system has capabilities for mass production of examinations, correction of examinations, and production of accumulative item analyses. Use of this computerized system has produced savings in terms of money, faculty time, and clerical assistance. Primarily, however, it has served to effectively relate instructional objectives in an educational program to the evaluation process."}
{"id": "PMID:474612", "title": "Setting up proper illumination on your microscope.", "content": "This article discusses the 13-step K\u00f6hler method for microscope illumination.", "contents": "Setting up proper illumination on your microscope. This article discusses the 13-step K\u00f6hler method for microscope illumination."}
{"id": "PMID:474616", "title": "The Summitt syndrome: observations on a third case.", "content": "A 6 1/2 year old male presented with acrocephaly, brachydactyly, clinodactyly, mild syndactyly of the hands and feet, genu valgum, and marked obesity. Roentgenograms of the hands revealed hypoplasia or aplasia of the middle phalanges. Roentgenograms of the feet revealed hypoplasia of the middle phalanges and deformity of the proximal phalangeal epiphyses of the great toes. Chromosomes studies revealed a normal 46,XY karyotype, and psychological testing revealed low normal intelligence. Current data support autosomal recessive inheritance, although X-linkage cannot be excluded.", "contents": "The Summitt syndrome: observations on a third case. A 6 1/2 year old male presented with acrocephaly, brachydactyly, clinodactyly, mild syndactyly of the hands and feet, genu valgum, and marked obesity. Roentgenograms of the hands revealed hypoplasia or aplasia of the middle phalanges. Roentgenograms of the feet revealed hypoplasia of the middle phalanges and deformity of the proximal phalangeal epiphyses of the great toes. Chromosomes studies revealed a normal 46,XY karyotype, and psychological testing revealed low normal intelligence. Current data support autosomal recessive inheritance, although X-linkage cannot be excluded."}
{"id": "PMID:474617", "title": "Autosomal dominant inheritance of scalp defects with ectrodactyly.", "content": "Nine members of four generations of a kindred had an autosomal dominant syndrome in which congenital scalp defects were associated with abnormalities of the hands and feet. Radiographically apparent, circumscribed defects of the skull were an additional inconsistent feature. Genetic counseling is made difficult by varying phenotypic expression of the gene.", "contents": "Autosomal dominant inheritance of scalp defects with ectrodactyly. Nine members of four generations of a kindred had an autosomal dominant syndrome in which congenital scalp defects were associated with abnormalities of the hands and feet. Radiographically apparent, circumscribed defects of the skull were an additional inconsistent feature. Genetic counseling is made difficult by varying phenotypic expression of the gene."}
{"id": "PMID:474618", "title": "Inherited interstitial del(Xp) with minimal clinical consequences: with a note on the location of genes controlling phenotypic features.", "content": "In a routine cytogenetic investigation of the outpatients of a hospital for the mentally retarded, a 26-year-old women with a presumptive interstitial deletion of the short arm of one of the X chromosomes was found. The same aberration was found in her phenotypically normal mother and in one of her four sisters, all phenotypically normal. By GTG- and QFQ-banding methods, the deletion was interpreted to involve the entire band Xp21 and adjacent parts of p11 and p22. The karyotype is written 46,X,del(X)(pter leads to p22::p11 leads to qter). By autoradiography and Bud R acridine orange technique, the deleted X was the late replicating one in all three affected persons. The deletion apparently causes shortness of stature but no other phenotypic symptoms or signs. Hence a gene or genes controlling stature is located in band Xp21 or regions immediately adjacent to this band. Since the absence of this region does not cause streak gonads, it does not contain genes controlling the formation of the ovaries. This appears to be the first example of a heritable chromosome deletion compatible with a normal phenotype and reproduction.", "contents": "Inherited interstitial del(Xp) with minimal clinical consequences: with a note on the location of genes controlling phenotypic features. In a routine cytogenetic investigation of the outpatients of a hospital for the mentally retarded, a 26-year-old women with a presumptive interstitial deletion of the short arm of one of the X chromosomes was found. The same aberration was found in her phenotypically normal mother and in one of her four sisters, all phenotypically normal. By GTG- and QFQ-banding methods, the deletion was interpreted to involve the entire band Xp21 and adjacent parts of p11 and p22. The karyotype is written 46,X,del(X)(pter leads to p22::p11 leads to qter). By autoradiography and Bud R acridine orange technique, the deleted X was the late replicating one in all three affected persons. The deletion apparently causes shortness of stature but no other phenotypic symptoms or signs. Hence a gene or genes controlling stature is located in band Xp21 or regions immediately adjacent to this band. Since the absence of this region does not cause streak gonads, it does not contain genes controlling the formation of the ovaries. This appears to be the first example of a heritable chromosome deletion compatible with a normal phenotype and reproduction."}
{"id": "PMID:474619", "title": "The G syndrome: a case report.", "content": "A case of the G syndrome is reported in a baby boy who had an unusual facies (prominent forehead, telecanthus, posteriorly rotated ears, and anteverted nostrils), laryngeal cleft, hypospadias, cryptorchidism, and psychomotor development delay, possibly secondary to birth asphyxia and numerous medical and surgical complications. The mother had a similar facies.", "contents": "The G syndrome: a case report. A case of the G syndrome is reported in a baby boy who had an unusual facies (prominent forehead, telecanthus, posteriorly rotated ears, and anteverted nostrils), laryngeal cleft, hypospadias, cryptorchidism, and psychomotor development delay, possibly secondary to birth asphyxia and numerous medical and surgical complications. The mother had a similar facies."}
{"id": "PMID:474620", "title": "Mutations affecting pigmentation in man: I. Neuroectodermal melanolysosomal disease.", "content": "We describe a syndrome identified in three consanguineous families who had two and probably four common ancestors five generations ago. The syndrome is characterized by profound dysfunction of the central nervous system, silver-leaden colored hair, abnormal melanosomes and melanocytes, and abnormal inclusion bodies in fibroblasts, bone marrow histiocytes and lymphocytes which appear to represent abnormal lysosomal bodies. Because of the biochemical relationships between melanin-melanosomes and neuromelanin, we think that all the manifestations of the condition are related to and represent pleiotropic effects of a newly identified gene in man in its homozygous state. Biochemical reactions of the cells of these patients indicate presence of tyrosinase in the melanosomes.and show that the substance accumulated in cultured fibroblasts and in the bone marrow histiocytes is a PAS and Oil-red-O positive material but is Oil-red-O negative after extraction; it has the typical reactions of melanin withe the Masson and Fontana stain, but cannot be considered typical melanin, since without stain it is colorless. The ultrastructural studies showed round granules with variable matrix, similar in fibroblast and bone marrow, and with variable intensity of reaction to osmium. This mutation principally affects the neuroectoderm, but also the mesoderm.", "contents": "Mutations affecting pigmentation in man: I. Neuroectodermal melanolysosomal disease. We describe a syndrome identified in three consanguineous families who had two and probably four common ancestors five generations ago. The syndrome is characterized by profound dysfunction of the central nervous system, silver-leaden colored hair, abnormal melanosomes and melanocytes, and abnormal inclusion bodies in fibroblasts, bone marrow histiocytes and lymphocytes which appear to represent abnormal lysosomal bodies. Because of the biochemical relationships between melanin-melanosomes and neuromelanin, we think that all the manifestations of the condition are related to and represent pleiotropic effects of a newly identified gene in man in its homozygous state. Biochemical reactions of the cells of these patients indicate presence of tyrosinase in the melanosomes.and show that the substance accumulated in cultured fibroblasts and in the bone marrow histiocytes is a PAS and Oil-red-O positive material but is Oil-red-O negative after extraction; it has the typical reactions of melanin withe the Masson and Fontana stain, but cannot be considered typical melanin, since without stain it is colorless. The ultrastructural studies showed round granules with variable matrix, similar in fibroblast and bone marrow, and with variable intensity of reaction to osmium. This mutation principally affects the neuroectoderm, but also the mesoderm."}
{"id": "PMID:474622", "title": "Finite sample properties of maximum likelihood estimates of the recombination fraction in double backcross matings in man.", "content": "The properties of the maximum likelihood estimator (MLE) of the recombination fraction, theta, based on various numbers and sizes of sibships derived from double backcross matings are exactly explored where the coupling phases are presumed equally likely (which for two-generation data is generally the case). The results indicate that for large values of theta the expectations are severely biased. The bias, variance, and measures of normality of the MLE behave erratically for small sizes and numbers of sibships. The implications for chromosome mapping are discussed.", "contents": "Finite sample properties of maximum likelihood estimates of the recombination fraction in double backcross matings in man. The properties of the maximum likelihood estimator (MLE) of the recombination fraction, theta, based on various numbers and sizes of sibships derived from double backcross matings are exactly explored where the coupling phases are presumed equally likely (which for two-generation data is generally the case). The results indicate that for large values of theta the expectations are severely biased. The bias, variance, and measures of normality of the MLE behave erratically for small sizes and numbers of sibships. The implications for chromosome mapping are discussed."}
{"id": "PMID:474623", "title": "Genetic and diagnostic considerations in three families with abnormalities of facial expression and congenital urinary obstruction: \"The Ochoa syndrome\".", "content": "Seven patients (4 females, and 3 males) born in unrelated families, one of them consanguineous (first cousins), were affected by peculiar facies and gestures while smiling and crying, and by hydronephrosis, hydroureter and intravesical stenosis of the ureter, abnormal caliber of the urether in the prostatic and membranous portions, urethral valves, abnormal bladder with trabeculation, and diverticula associated with severe hypertrophy of the mucosa with sclerotic changes. The genetic analysis of these families indicates that the condition is probably autosomal dominant, with variable expressivity and incomplete penetrance. The syndrome represents alteration of facial and urinary developmental fields. The peculiar facies allows early recognition of the condition, and this can be helpful for early assessment and treatment, leading perhaps to a better prognosis.", "contents": "Genetic and diagnostic considerations in three families with abnormalities of facial expression and congenital urinary obstruction: \"The Ochoa syndrome\". Seven patients (4 females, and 3 males) born in unrelated families, one of them consanguineous (first cousins), were affected by peculiar facies and gestures while smiling and crying, and by hydronephrosis, hydroureter and intravesical stenosis of the ureter, abnormal caliber of the urether in the prostatic and membranous portions, urethral valves, abnormal bladder with trabeculation, and diverticula associated with severe hypertrophy of the mucosa with sclerotic changes. The genetic analysis of these families indicates that the condition is probably autosomal dominant, with variable expressivity and incomplete penetrance. The syndrome represents alteration of facial and urinary developmental fields. The peculiar facies allows early recognition of the condition, and this can be helpful for early assessment and treatment, leading perhaps to a better prognosis."}
{"id": "PMID:474624", "title": "Genetic counseling: provision and reception of information.", "content": "To explore verbal communication between family and counselor, transcripts of 30 tape-recorded or observed genetic counseling sessions are analyzed qualitatively. In half the cases, available data did not allow counselors to give parents a single estimate of the recurrence rate. Moreover, limits on the language available for communicating small probabilities made common the use of nonnumerical statements about a family's chance of having an abnormal child. Counselees processed the factual information they were given, most commonly translating recurrence rates in ways that emphasized the uncertainties associated with them. They tended to view these rates in binary form and requested guidelines for their behavior, indicating uncertainty about how to proceed with reproductive decision-making. The findings suggest that strategies for processing information are an important element influencing parental perspectives on and approaches to the problems created by being at-risk and to possible courses of action.", "contents": "Genetic counseling: provision and reception of information. To explore verbal communication between family and counselor, transcripts of 30 tape-recorded or observed genetic counseling sessions are analyzed qualitatively. In half the cases, available data did not allow counselors to give parents a single estimate of the recurrence rate. Moreover, limits on the language available for communicating small probabilities made common the use of nonnumerical statements about a family's chance of having an abnormal child. Counselees processed the factual information they were given, most commonly translating recurrence rates in ways that emphasized the uncertainties associated with them. They tended to view these rates in binary form and requested guidelines for their behavior, indicating uncertainty about how to proceed with reproductive decision-making. The findings suggest that strategies for processing information are an important element influencing parental perspectives on and approaches to the problems created by being at-risk and to possible courses of action."}
{"id": "PMID:474625", "title": "The Johanson-Blizzard syndrome: case report and autopsy findings.", "content": "We report the case of a boy with the Johanson-Blizzard syndrome who died at the age of 8 years with complications of pancreatic exocrine insufficiency, and at autopsy was found to have a small thyroid filled with colloid, virtually complete replacement of the pancreas with adipose tissue, and a brain of normal size but with evidence of a cortical developmental defect consisting of abnormalities of gyral formation and of cortical neuronal organization. In addition the boy had postnatal growth failure, apparent severe mental retardation, congenital scalp defects and scalp hair patterning abnormalities, aplasia of the nasal alae, nasolacrimo-cutaneous fistulae, hypotonia, severe congenital sensorineural deafness, and small conical and widely spaced teeth. Evidence is accumulating that this syndrome is likely to be inherited as an autosomal recessive disorder. Our case represents the first report of autopsy findings in the syndrome.", "contents": "The Johanson-Blizzard syndrome: case report and autopsy findings. We report the case of a boy with the Johanson-Blizzard syndrome who died at the age of 8 years with complications of pancreatic exocrine insufficiency, and at autopsy was found to have a small thyroid filled with colloid, virtually complete replacement of the pancreas with adipose tissue, and a brain of normal size but with evidence of a cortical developmental defect consisting of abnormalities of gyral formation and of cortical neuronal organization. In addition the boy had postnatal growth failure, apparent severe mental retardation, congenital scalp defects and scalp hair patterning abnormalities, aplasia of the nasal alae, nasolacrimo-cutaneous fistulae, hypotonia, severe congenital sensorineural deafness, and small conical and widely spaced teeth. Evidence is accumulating that this syndrome is likely to be inherited as an autosomal recessive disorder. Our case represents the first report of autopsy findings in the syndrome."}
{"id": "PMID:474626", "title": "Increased aneuploidy in Alzheimer disease.", "content": "The purpose of this study was to determine if cytogenetic changes are present in Alzheimer disease, one of the presenile dementias. The chromosomes of three groups of people were studied: 1) sporadic cases of Alzheimer disease (eight cases), 2) familial cases of Alzheimer disease with affected individuals in at least two generations of their families (five cases), and 3) currently unaffected siblings of the affected individuals in these families (nine cases). One hundred cells per individual were examined using GTG banding to allow chromosome identification. A statistically significant increase in aneuploidy was found in five of eight patients in group 1 (P less than 0.05) and in each of five patients in group 2 (P less than 0.001) when compared with the rate of aneuploidy in age- and sex-matched controls. In addition, two individuals in group 3 exhibited a significant increase in aneuploidy over the control group, raising the possibility that finding increased aneuploidy may allow one to anticipate the clinical expression of the disease state.", "contents": "Increased aneuploidy in Alzheimer disease. The purpose of this study was to determine if cytogenetic changes are present in Alzheimer disease, one of the presenile dementias. The chromosomes of three groups of people were studied: 1) sporadic cases of Alzheimer disease (eight cases), 2) familial cases of Alzheimer disease with affected individuals in at least two generations of their families (five cases), and 3) currently unaffected siblings of the affected individuals in these families (nine cases). One hundred cells per individual were examined using GTG banding to allow chromosome identification. A statistically significant increase in aneuploidy was found in five of eight patients in group 1 (P less than 0.05) and in each of five patients in group 2 (P less than 0.001) when compared with the rate of aneuploidy in age- and sex-matched controls. In addition, two individuals in group 3 exhibited a significant increase in aneuploidy over the control group, raising the possibility that finding increased aneuploidy may allow one to anticipate the clinical expression of the disease state."}
{"id": "PMID:474627", "title": "Chylous ascites in sibs from a consanguineous marriage.", "content": "We report a brother and sister with congenital chylous ascites. Parents were first cousins, and autosomal recessive inheritance may be the cause of the condition in these sibs.", "contents": "Chylous ascites in sibs from a consanguineous marriage. We report a brother and sister with congenital chylous ascites. Parents were first cousins, and autosomal recessive inheritance may be the cause of the condition in these sibs."}
{"id": "PMID:474628", "title": "H-Y antigen in 46,XY pure testicular dysgenesis.", "content": "Clinical, cytogenetic, pathologic, and histocompatibility-Y (H-Y) antigen studies were performed on a phenotype female with primary amenorrhea and streak gonads. Pathological examination of tissues removed at total hysterectomy and bilateral salpinog-gonadectomy showed gonadoblastoma and dysgerminoma of left streak. A single F-body (Y chromosome) was found in buccal smears. Analysis of blood cells and tumor fibroblasts showed a 46,XY chromosome constitution (Q-banding). The data were consistent with a diagnosis of 46,XY pure testicular dysgenesis. Positive results for H-Y antigen were found in this case.", "contents": "H-Y antigen in 46,XY pure testicular dysgenesis. Clinical, cytogenetic, pathologic, and histocompatibility-Y (H-Y) antigen studies were performed on a phenotype female with primary amenorrhea and streak gonads. Pathological examination of tissues removed at total hysterectomy and bilateral salpinog-gonadectomy showed gonadoblastoma and dysgerminoma of left streak. A single F-body (Y chromosome) was found in buccal smears. Analysis of blood cells and tumor fibroblasts showed a 46,XY chromosome constitution (Q-banding). The data were consistent with a diagnosis of 46,XY pure testicular dysgenesis. Positive results for H-Y antigen were found in this case."}
{"id": "PMID:474629", "title": "Syndromes associated with deletion of the long arm of chromosome 18[del(18q)].", "content": "We studied eight persons whose karyotypes demonstrated deletion of a portion of the long arm of chromosome 18. Seven of these persons who showed the typical del(18q) syndrome had a common deletion in band 18q21, most likely band q21.3, and in at least two persons the deletion was interstitial. Another mentally retarded child, dissimilar in appearance, had a more proximal deletion within band 18q12. Two different clinical syndromes resulted from deletions of these different segments of the long arm of chromosome 18.", "contents": "Syndromes associated with deletion of the long arm of chromosome 18[del(18q)]. We studied eight persons whose karyotypes demonstrated deletion of a portion of the long arm of chromosome 18. Seven of these persons who showed the typical del(18q) syndrome had a common deletion in band 18q21, most likely band q21.3, and in at least two persons the deletion was interstitial. Another mentally retarded child, dissimilar in appearance, had a more proximal deletion within band 18q12. Two different clinical syndromes resulted from deletions of these different segments of the long arm of chromosome 18."}
{"id": "PMID:474630", "title": "An examination of the spectrum of anatomic defects and variations found in eight cases of trisomy 13.", "content": "We report the anatomic variations found in four additional cases of trisomy 13. Data from these and four previous cases [Colacino and Pettersen, 1978] are utilized to define a muscle phenotype. Previously unreported defects include the bilateral presence of cervical ribs and the bilateral absence of 12th thoracic ribs in five of the eight cases. One unusual developmental defect of the great vessels is also described. The findings suggest that a definitive diagnosis of trisomy 13 can be made on the basis of six muscle variations.", "contents": "An examination of the spectrum of anatomic defects and variations found in eight cases of trisomy 13. We report the anatomic variations found in four additional cases of trisomy 13. Data from these and four previous cases [Colacino and Pettersen, 1978] are utilized to define a muscle phenotype. Previously unreported defects include the bilateral presence of cervical ribs and the bilateral absence of 12th thoracic ribs in five of the eight cases. One unusual developmental defect of the great vessels is also described. The findings suggest that a definitive diagnosis of trisomy 13 can be made on the basis of six muscle variations."}
{"id": "PMID:474631", "title": "The hand profile on de Lange syndrome: diagnostic criteria.", "content": "The hand pattern profiles of 18 previously published and 10 new cases of de Lange syndrome were compared to those of cases referred as suspects, but judged clinically and by numerical taxonomic methods not to have de Lange syndrome (non-de Lange). Based on a Poznanski metacarpophalangeal profile of the 2 groups of patients, a simple scoring system using 11 measurements was devised using the 16 most marked differences within and between metacarpals and phalanges. Of the metacarpals, the first is shorter than the second, third, fourth, or fifth; and the second and fifth are shorter than the third or fourth. Of the middle phalanges, the third and fourth are shorter than the respective metacarpal and than the second and fifth middle phalanx. Of the distal phalanges, the fifth is shorter than the second. The mean Z score for these 11 measurements is further below normal in the de Lange group than in the non-de Lange group. All 11 non-de Lange patients had a score less than 17, and all 28 de Lange patients had a score of 18 or more.", "contents": "The hand profile on de Lange syndrome: diagnostic criteria. The hand pattern profiles of 18 previously published and 10 new cases of de Lange syndrome were compared to those of cases referred as suspects, but judged clinically and by numerical taxonomic methods not to have de Lange syndrome (non-de Lange). Based on a Poznanski metacarpophalangeal profile of the 2 groups of patients, a simple scoring system using 11 measurements was devised using the 16 most marked differences within and between metacarpals and phalanges. Of the metacarpals, the first is shorter than the second, third, fourth, or fifth; and the second and fifth are shorter than the third or fourth. Of the middle phalanges, the third and fourth are shorter than the respective metacarpal and than the second and fifth middle phalanx. Of the distal phalanges, the fifth is shorter than the second. The mean Z score for these 11 measurements is further below normal in the de Lange group than in the non-de Lange group. All 11 non-de Lange patients had a score less than 17, and all 28 de Lange patients had a score of 18 or more."}
{"id": "PMID:474632", "title": "Duplication 6q syndrome.", "content": "Duplication (partial trisomy) of the long arm of chromosome 6 has been described in 5 children [Robertson et al, 1975, Chen et al, 1976, Clark, 1977]. We wish to report here an additional case due to a familial translocation in which the proband's karyotype is 46,XX,der(3),rcp(3;6)(p25;q21)mat. The phenotypes of the 6 children with duplication 6q are strikingly similar. Each child has duplication involving approximately the distal 1/3 to 1/2 of the long arm of chromosome 6. Distinctive features present in all 6 children include microcephaly, acrocephaly, prominent forehead, flat facial profile, depressed nasal bridge, flat malar areas, \"carp\" mouth, micrognathia and mental retardation. The phenotype of the duplication 6q syndrome is distinctive enough to be clinically recognizable.", "contents": "Duplication 6q syndrome. Duplication (partial trisomy) of the long arm of chromosome 6 has been described in 5 children [Robertson et al, 1975, Chen et al, 1976, Clark, 1977]. We wish to report here an additional case due to a familial translocation in which the proband's karyotype is 46,XX,der(3),rcp(3;6)(p25;q21)mat. The phenotypes of the 6 children with duplication 6q are strikingly similar. Each child has duplication involving approximately the distal 1/3 to 1/2 of the long arm of chromosome 6. Distinctive features present in all 6 children include microcephaly, acrocephaly, prominent forehead, flat facial profile, depressed nasal bridge, flat malar areas, \"carp\" mouth, micrognathia and mental retardation. The phenotype of the duplication 6q syndrome is distinctive enough to be clinically recognizable."}
{"id": "PMID:474633", "title": "Trisomy 14 mosaicism: case report and review.", "content": "Complete or partial trisomy 14 is compatible with life. However, in the former case, mosaicism is probably always present. A case of trisomy 14 mosaicism is reported. Comparisons are made with other trisomy 14, trisomy 14 mosaicism, and duplication 14q cases previously reported. As a group, they share some clinical manifestations. The phenotype consists of multiple congenital anomalies, including microcephaly, broad nose, wide mouth, high or cleft palate, micrognathia, congenital heart disease, intrauterine growth retardation, and mental retardation. The present patient also has asthma, eczema, and developmental asymmetry.", "contents": "Trisomy 14 mosaicism: case report and review. Complete or partial trisomy 14 is compatible with life. However, in the former case, mosaicism is probably always present. A case of trisomy 14 mosaicism is reported. Comparisons are made with other trisomy 14, trisomy 14 mosaicism, and duplication 14q cases previously reported. As a group, they share some clinical manifestations. The phenotype consists of multiple congenital anomalies, including microcephaly, broad nose, wide mouth, high or cleft palate, micrognathia, congenital heart disease, intrauterine growth retardation, and mental retardation. The present patient also has asthma, eczema, and developmental asymmetry."}
{"id": "PMID:474634", "title": "Cytogenetic studies of a male with sporadic intestinal lymphangiectasia: 45,X/46,XY mosaicism with pseudo- and hyperdiploid subpopulations in cultured tissues.", "content": "45,X/46,XY mosaicism was found in peripheral blood, bone marrow, and tissue cultures of an adult male with intestinal lymphangiectasia (IL). Turner phenotype was not present; his meiotic metaphase analysis was normak, and his dermatoglyphics resembled those of his family. Ten separate tissue culture lines from three biopsies of skin and thyroid gland contained 45,X cells (14.8 to 78.3%). Autosomal aneuploidy, resulting in pseudo- or hyperdiploidy, was also present in 4.3 to 41.6% of the cells. A hyperdiploid clone with a 47,X,+10,+18 karyotype was found in 22.6% of cells in one line. A second hyperdiploid clone with a 48,X,+2,+18,+18 karyotype occurred in 7.6% of cells from another line containing a total of 41.6% pseudo- and hyperdiploid cells. Such clonal abnormalities were not typical of tissue cultures from other patients done in our laboratory. Growth of our patient's tissue cultures was subnormal, and none proliferated beyond the fourth subculture. The significance of this observation remains to be determined. Our results do not allow us to conclude whether our patient's mosaicism of somatic tissues arose during embryogenesis, or whether it originated post-natally. The secondary immunodeficiency which occurs in IL may explain persistence of cells with unusual combinations of autosomal aneuploidy in our patient's tissues.", "contents": "Cytogenetic studies of a male with sporadic intestinal lymphangiectasia: 45,X/46,XY mosaicism with pseudo- and hyperdiploid subpopulations in cultured tissues. 45,X/46,XY mosaicism was found in peripheral blood, bone marrow, and tissue cultures of an adult male with intestinal lymphangiectasia (IL). Turner phenotype was not present; his meiotic metaphase analysis was normak, and his dermatoglyphics resembled those of his family. Ten separate tissue culture lines from three biopsies of skin and thyroid gland contained 45,X cells (14.8 to 78.3%). Autosomal aneuploidy, resulting in pseudo- or hyperdiploidy, was also present in 4.3 to 41.6% of the cells. A hyperdiploid clone with a 47,X,+10,+18 karyotype was found in 22.6% of cells in one line. A second hyperdiploid clone with a 48,X,+2,+18,+18 karyotype occurred in 7.6% of cells from another line containing a total of 41.6% pseudo- and hyperdiploid cells. Such clonal abnormalities were not typical of tissue cultures from other patients done in our laboratory. Growth of our patient's tissue cultures was subnormal, and none proliferated beyond the fourth subculture. The significance of this observation remains to be determined. Our results do not allow us to conclude whether our patient's mosaicism of somatic tissues arose during embryogenesis, or whether it originated post-natally. The secondary immunodeficiency which occurs in IL may explain persistence of cells with unusual combinations of autosomal aneuploidy in our patient's tissues."}
{"id": "PMID:474638", "title": "Gerodermia osteodysplastica hereditaria: report of three affected brothers and literature review.", "content": "Gerodermia osteodysplastica hereditaria was diagnosed in three Mexican brothers 6, 7, and 8 years old, respectively, who had the distinct facial appearance with sagging cheeks, premature wrinkling of the skin of face, abdomen, and dorsum of hands and feet; malocclusion, span greater than height; hyperextensibility; winging of the scapulae; stooped posture with kyphoscoliosis; protuberant abdomen; and pes planus. Radiologically they had generalized osteoplorosis, platyspondily due to multiple compression fractures, pseudoepiphyses of second metacarpals, and dislocated hips. Three other families with a total of 14 affected individuals have been reported. Inter- and intrafamilial variability can be recognized, particularly regarding the tendency to fractures, upper:lower segment ratio abnormalities, and results of skin biopsies, which have shown fragmentation of the elastic fibers in some cases (including the present family) and not in others. Although inheritance was considered to be X-linked recessive in the first reported family, an analysis of that pedigree together with those of the other reported families, including the present one, suggests that gerodermia osteodrysplastica is inherited in an autosomal recessive manner.", "contents": "Gerodermia osteodysplastica hereditaria: report of three affected brothers and literature review. Gerodermia osteodysplastica hereditaria was diagnosed in three Mexican brothers 6, 7, and 8 years old, respectively, who had the distinct facial appearance with sagging cheeks, premature wrinkling of the skin of face, abdomen, and dorsum of hands and feet; malocclusion, span greater than height; hyperextensibility; winging of the scapulae; stooped posture with kyphoscoliosis; protuberant abdomen; and pes planus. Radiologically they had generalized osteoplorosis, platyspondily due to multiple compression fractures, pseudoepiphyses of second metacarpals, and dislocated hips. Three other families with a total of 14 affected individuals have been reported. Inter- and intrafamilial variability can be recognized, particularly regarding the tendency to fractures, upper:lower segment ratio abnormalities, and results of skin biopsies, which have shown fragmentation of the elastic fibers in some cases (including the present family) and not in others. Although inheritance was considered to be X-linked recessive in the first reported family, an analysis of that pedigree together with those of the other reported families, including the present one, suggests that gerodermia osteodrysplastica is inherited in an autosomal recessive manner."}
{"id": "PMID:474639", "title": "Effect of diabetes mellitus on amniotic fluid lecithin/sphingomyelin ratio and respiratory distress syndrome.", "content": "Ninety-eight insulin-dependent diabetic patients were cared for during 108 pregnancies following an ambulatory program aimed at close control of blood sugar levels. The incidence of respiratory distress syndrome (RDS) in these patients was not different from that in nondiabetic patients. Fetal pulmonary maturity seemed to progress at a normal rate as evidenced by an equal incidence of mature lecithin/sphingomyelin (L/S) ratios at various stages of gestation in diabetic and nondiabetic patients. There was no evidence that Class B and C diabetic patients have delayed fetal lung maturation or that Class E, F, and R patients have accelerated maturation. It is concluded that careful metabolic control of the pregnant diabetic patient results in a normal process of fetal pulmonary maturation without increased risk of RDS.", "contents": "Effect of diabetes mellitus on amniotic fluid lecithin/sphingomyelin ratio and respiratory distress syndrome. Ninety-eight insulin-dependent diabetic patients were cared for during 108 pregnancies following an ambulatory program aimed at close control of blood sugar levels. The incidence of respiratory distress syndrome (RDS) in these patients was not different from that in nondiabetic patients. Fetal pulmonary maturity seemed to progress at a normal rate as evidenced by an equal incidence of mature lecithin/sphingomyelin (L/S) ratios at various stages of gestation in diabetic and nondiabetic patients. There was no evidence that Class B and C diabetic patients have delayed fetal lung maturation or that Class E, F, and R patients have accelerated maturation. It is concluded that careful metabolic control of the pregnant diabetic patient results in a normal process of fetal pulmonary maturation without increased risk of RDS."}
{"id": "PMID:474640", "title": "Relief of dysmenorrhea with the prostaglandin synthetase inhibitor ibuprofen: effect on prostaglandin levels in menstrual fluid.", "content": "The prostaglandin synthetase inhibitor ibuprofen was evaluated for relief of severe primary dysmenorrhea in a controlled, double-blind, cross-over study in seven patients for a total of 23 menstrual cycles. In eight untreated cycles, the amount of prostaglandin (PG) in the menstrual fluid was higher than in nondysmenorrheic subjects. There was good to excellent relief of dysmenorrhea in seven ibuprofen-treated cycles, which was associated with a threefold to fourfold reduction in menstrual PG released. When a placebo was given in five cycles, only poor or minimal relief of dysmenorrhea was obtained and the menstrual PG released was similar to that in control cycles. In individual patients, there was a remarkable correlation between the severity of menstrual pain as assessed daily by the patient and the level of menstrual PG released during the corresponding period. The effect of ibuprofen therapy on menstrual fluid volume was inconsistent. The study shows that in severe primary dysmenorrhea there is increased release of PG in the menstrual fluid; this can be effectively suppressed with ibuprofen, which provides excellent relief from the symptoms of dysmenorrhea.", "contents": "Relief of dysmenorrhea with the prostaglandin synthetase inhibitor ibuprofen: effect on prostaglandin levels in menstrual fluid. The prostaglandin synthetase inhibitor ibuprofen was evaluated for relief of severe primary dysmenorrhea in a controlled, double-blind, cross-over study in seven patients for a total of 23 menstrual cycles. In eight untreated cycles, the amount of prostaglandin (PG) in the menstrual fluid was higher than in nondysmenorrheic subjects. There was good to excellent relief of dysmenorrhea in seven ibuprofen-treated cycles, which was associated with a threefold to fourfold reduction in menstrual PG released. When a placebo was given in five cycles, only poor or minimal relief of dysmenorrhea was obtained and the menstrual PG released was similar to that in control cycles. In individual patients, there was a remarkable correlation between the severity of menstrual pain as assessed daily by the patient and the level of menstrual PG released during the corresponding period. The effect of ibuprofen therapy on menstrual fluid volume was inconsistent. The study shows that in severe primary dysmenorrhea there is increased release of PG in the menstrual fluid; this can be effectively suppressed with ibuprofen, which provides excellent relief from the symptoms of dysmenorrhea."}
{"id": "PMID:474641", "title": "Morphologic features of prognostic significance in uterine smooth muscle tumors: a review of eighty-four cases.", "content": "Independent microscopic review of the most cellular and pleomorphic areas of 84 smooth muscle tumors of the uterus by two pathologists yielded 23 leiomyosarcomas, 43 cellular leiomyomas, 16 bizarre leiomyomas, and two intravenous leiomyomas. Greater than 5 mitotic figures/10 high-power fields (hpf) correlated with malignant clinical behavior, but this occurred in only 75% of the leiomyosarcomas. Five cases with fewer than 5 mitoses/10 hpf pursued a malignant course. The mean age of the patients with malignant tumors (52.5 years) was similar to the mean age of those with bizarre leiomyomas (48.0 years) but those patients with cellular leiomyomas were significantly younger (42.0 years; p less than 0.01). Gross evidence of extrauterine extension was diagnostic of malignancy as was vascular invasion by anaplastic tumor. Fifteen of the 19 patients with leiomyosarcoma died of disease at an average of 28 months postoperatively. Inadequate sampling and interobserver variation are discussed.", "contents": "Morphologic features of prognostic significance in uterine smooth muscle tumors: a review of eighty-four cases. Independent microscopic review of the most cellular and pleomorphic areas of 84 smooth muscle tumors of the uterus by two pathologists yielded 23 leiomyosarcomas, 43 cellular leiomyomas, 16 bizarre leiomyomas, and two intravenous leiomyomas. Greater than 5 mitotic figures/10 high-power fields (hpf) correlated with malignant clinical behavior, but this occurred in only 75% of the leiomyosarcomas. Five cases with fewer than 5 mitoses/10 hpf pursued a malignant course. The mean age of the patients with malignant tumors (52.5 years) was similar to the mean age of those with bizarre leiomyomas (48.0 years) but those patients with cellular leiomyomas were significantly younger (42.0 years; p less than 0.01). Gross evidence of extrauterine extension was diagnostic of malignancy as was vascular invasion by anaplastic tumor. Fifteen of the 19 patients with leiomyosarcoma died of disease at an average of 28 months postoperatively. Inadequate sampling and interobserver variation are discussed."}
{"id": "PMID:474642", "title": "Correlation of ultrasonic and endocrinologic assessment of human follicular development.", "content": "Human ovarian follicular development measured by peripheral plasma hormone levels and ovarian follicular growth assessed by ultrasonic visualization of the ovary were compared and contrasted in apparently normally menstruating women. The linear correlation coefficient between the mean values for plasma estradiol and follicular diameter on day -5 to day 0 of 15 normal cycles was r = 0.968. When the individual paired data were subjected to multiple linear regression analyses (on days -5 to 0), r = 0.771. These good correlations show that ultrasound measurements of follicular diameter are valuable in the assessment of follicular growth and development. Observations on apparently abnormal cycles are also discussed. Ultrasound measurement of follicular diameter offers an additional technique for the study of ovarian function.", "contents": "Correlation of ultrasonic and endocrinologic assessment of human follicular development. Human ovarian follicular development measured by peripheral plasma hormone levels and ovarian follicular growth assessed by ultrasonic visualization of the ovary were compared and contrasted in apparently normally menstruating women. The linear correlation coefficient between the mean values for plasma estradiol and follicular diameter on day -5 to day 0 of 15 normal cycles was r = 0.968. When the individual paired data were subjected to multiple linear regression analyses (on days -5 to 0), r = 0.771. These good correlations show that ultrasound measurements of follicular diameter are valuable in the assessment of follicular growth and development. Observations on apparently abnormal cycles are also discussed. Ultrasound measurement of follicular diameter offers an additional technique for the study of ovarian function."}
{"id": "PMID:474643", "title": "The effect of oral contraceptives on vitamin B12 metabolism.", "content": "Serum vitamin vitamin B12 levels were determined in 199 women who were on a regimen of oral contraceptives of either the combination or sequential type and in a control group of 196 women. The group using oral contraceptive agents (OCA) had significantly lower serum levels of vitamin B12 vitamin as compared to those of the control group. A total of 19 women using OCAs had serum vitamin B12 levels that were lower than normal values. However, the Schilling test and urinary methylmalonate excretion in this group were normal. The OCA group had a significantly lower total serum vitamin B12 binding capacity, a lower total transcobalamin I level, and a higher transcobalamin III level. Our study suggests that the fall of serum vitamin B12 in OCA users is due to the changes in vitamin B12 binders of serum and does not represent vitamin B12 deficiency. Therefore, according to our data, there is no justification for vitamin B12 supplementation in users of oral contraceptives.", "contents": "The effect of oral contraceptives on vitamin B12 metabolism. Serum vitamin vitamin B12 levels were determined in 199 women who were on a regimen of oral contraceptives of either the combination or sequential type and in a control group of 196 women. The group using oral contraceptive agents (OCA) had significantly lower serum levels of vitamin B12 vitamin as compared to those of the control group. A total of 19 women using OCAs had serum vitamin B12 levels that were lower than normal values. However, the Schilling test and urinary methylmalonate excretion in this group were normal. The OCA group had a significantly lower total serum vitamin B12 binding capacity, a lower total transcobalamin I level, and a higher transcobalamin III level. Our study suggests that the fall of serum vitamin B12 in OCA users is due to the changes in vitamin B12 binders of serum and does not represent vitamin B12 deficiency. Therefore, according to our data, there is no justification for vitamin B12 supplementation in users of oral contraceptives."}
{"id": "PMID:474644", "title": "How does one assess the risk of abnormalities from human in vitro fertilization?", "content": "The procedure of in vitro fertilization, including ova collection and embryo culture and transfer, raises concern about the risk of abnormalities arising from the technique. In vitro versus in vivo comparison might be made in the study of preimplantation embryos and spontaneous abortions, the results of amniocentesis, and the findings among fetal deaths and live births. In vivo, 40% to 50% of implantation blastocysts are estimated to have a chromosomal abnormality, over 99% of which are estimated to be eliminated during the course of pregnancy. In principle, the use of early spontaneous abortions can be more efficient than amniocentesis for detecting an increased risk of chromosomal abnormalities at birth. Unless in vitro fertilization in humans strongly contradicts the experience in domestic animal reproduction, which suggests no increased risk of abnormalities at birth, a large number of births would be required to provide a definitive assessment of risk.", "contents": "How does one assess the risk of abnormalities from human in vitro fertilization? The procedure of in vitro fertilization, including ova collection and embryo culture and transfer, raises concern about the risk of abnormalities arising from the technique. In vitro versus in vivo comparison might be made in the study of preimplantation embryos and spontaneous abortions, the results of amniocentesis, and the findings among fetal deaths and live births. In vivo, 40% to 50% of implantation blastocysts are estimated to have a chromosomal abnormality, over 99% of which are estimated to be eliminated during the course of pregnancy. In principle, the use of early spontaneous abortions can be more efficient than amniocentesis for detecting an increased risk of chromosomal abnormalities at birth. Unless in vitro fertilization in humans strongly contradicts the experience in domestic animal reproduction, which suggests no increased risk of abnormalities at birth, a large number of births would be required to provide a definitive assessment of risk."}
{"id": "PMID:474657", "title": "Dimensions of prenatal anxiety and their influence on pregnancy outcome.", "content": "Wtih the use of perinatal data from a prospective study of 73 primigravid women and their newborn infants, we determined that prenatal anxiety is not a unitary construct, but that it is useful to consider the distinct concerns of pregnancy. From interviews with the pregnant women, three dimensions of anxiety were identified: (1) anxiety about the pregnancy and approaching birth, (2) anxiety about anticipated care of the child, and (3) psychiatric symptomatology. Correlations of the three anxiety dimensions with background and perinatal outcome variables reveal the strongest associations between anxiety about pregnancy and birth with maternal age, education, and preparation for childbirth. Anxieties about pregnancy and birth and about parenting are related to administration of anesthesia during childbirth and the motor maturity of the neonate. The findings thus give evidence that even in a medically uncomplicated population, emotional states of pregnancy are rooted in the woman's background and may have an impact on perinatal events and infant functioning.", "contents": "Dimensions of prenatal anxiety and their influence on pregnancy outcome. Wtih the use of perinatal data from a prospective study of 73 primigravid women and their newborn infants, we determined that prenatal anxiety is not a unitary construct, but that it is useful to consider the distinct concerns of pregnancy. From interviews with the pregnant women, three dimensions of anxiety were identified: (1) anxiety about the pregnancy and approaching birth, (2) anxiety about anticipated care of the child, and (3) psychiatric symptomatology. Correlations of the three anxiety dimensions with background and perinatal outcome variables reveal the strongest associations between anxiety about pregnancy and birth with maternal age, education, and preparation for childbirth. Anxieties about pregnancy and birth and about parenting are related to administration of anesthesia during childbirth and the motor maturity of the neonate. The findings thus give evidence that even in a medically uncomplicated population, emotional states of pregnancy are rooted in the woman's background and may have an impact on perinatal events and infant functioning."}
{"id": "PMID:474659", "title": "Weight gain and the outcome of pregnancy.", "content": "This study determined the relationship of maternal weight gain in pregnancy to its outcome. Mothers who were overweight at the start of pregnancy had the fewest fetal and neonatal deaths with a 16 pound weight gain at term. The optimal weight gain for normally proportioned mothers was 20 pounds and for underweight mothers 30 pounds. For all three groups perinatal mortality rates increased with weight gains less or more than these optimal values. Very low or very high pregnancy weight gains had only a modest influence on the frequency of common placental and fetal disorders. However, once one of these disorders was established, mortality rates from it usually increased severalfold when mothers had very low or very high weight gains.", "contents": "Weight gain and the outcome of pregnancy. This study determined the relationship of maternal weight gain in pregnancy to its outcome. Mothers who were overweight at the start of pregnancy had the fewest fetal and neonatal deaths with a 16 pound weight gain at term. The optimal weight gain for normally proportioned mothers was 20 pounds and for underweight mothers 30 pounds. For all three groups perinatal mortality rates increased with weight gains less or more than these optimal values. Very low or very high pregnancy weight gains had only a modest influence on the frequency of common placental and fetal disorders. However, once one of these disorders was established, mortality rates from it usually increased severalfold when mothers had very low or very high weight gains."}
{"id": "PMID:474660", "title": "Serial relaxin concentrations in human pregnancy.", "content": "Relaxin is a peptide hormone produced by the corpus luteum during pregnancy in human beings. There are no extraluteal sites of production of relaxin in women. By means of a heterologous porcine radioimmunoassay, serial serum relaxin concentrations were determined in three women throughout pregnancy and into labor. All three demonstrated a first-trimester elevation followed by a second-trimester decline; values then remained stable throughout the second and third trimesters and into labor. This further confirms the functional activity of the corpus luteum throughout pregnancy and the value of relaxin as an index of luteal function during gestation.", "contents": "Serial relaxin concentrations in human pregnancy. Relaxin is a peptide hormone produced by the corpus luteum during pregnancy in human beings. There are no extraluteal sites of production of relaxin in women. By means of a heterologous porcine radioimmunoassay, serial serum relaxin concentrations were determined in three women throughout pregnancy and into labor. All three demonstrated a first-trimester elevation followed by a second-trimester decline; values then remained stable throughout the second and third trimesters and into labor. This further confirms the functional activity of the corpus luteum throughout pregnancy and the value of relaxin as an index of luteal function during gestation."}
{"id": "PMID:474661", "title": "Prenatal diagnosis of the hemoglobinopathies: ethical issues.", "content": "Prenatal diagnosis of the hemoglobinopathies has been increasingly reported. This article discusses those ethical issues stemming from pregnancy studies by fetoscopy or placental aspiration: diagnostic accuracy and safety for mother and fetus, abortion of the fetus diagnosed as homozygous for thalassemia or sickle cell diseases, and access to prenatal diagnosis for those who cannot afford it. The author's position on these issues attempts to mediate between the poles in current ethical debate on abortion in public policy.", "contents": "Prenatal diagnosis of the hemoglobinopathies: ethical issues. Prenatal diagnosis of the hemoglobinopathies has been increasingly reported. This article discusses those ethical issues stemming from pregnancy studies by fetoscopy or placental aspiration: diagnostic accuracy and safety for mother and fetus, abortion of the fetus diagnosed as homozygous for thalassemia or sickle cell diseases, and access to prenatal diagnosis for those who cannot afford it. The author's position on these issues attempts to mediate between the poles in current ethical debate on abortion in public policy."}
{"id": "PMID:474662", "title": "The lung profile. I. Normal pregnancy.", "content": "The lung profile run by two-dimensional thin-layer chromatography is described, including the L/S ratio and the percentages of disaturated acetone precipitated lecithin, PI, and PG. Results show that the accuracy of this profile increases that of the mature L/S ratio value even further, while decreasing the missed predictions with low L/S ratios to only about 7%.", "contents": "The lung profile. I. Normal pregnancy. The lung profile run by two-dimensional thin-layer chromatography is described, including the L/S ratio and the percentages of disaturated acetone precipitated lecithin, PI, and PG. Results show that the accuracy of this profile increases that of the mature L/S ratio value even further, while decreasing the missed predictions with low L/S ratios to only about 7%."}
{"id": "PMID:474663", "title": "Origin of the alkaline phosphatases in amniotic fluid.", "content": "The nature and origins of amniotic fluid ALP activity were investigated early (14 to 22 weeks) and late (25 to 44 weeks) in pregnancy. The total enzyme activities for both stages were significantly different and considerable changes in the activities of individual enzyme components occurred. Early activity consists of intestinal (81%), bone/liver/kidney (15%), and placental (4%) ALP. In late fluids, the values are 5%, 69% and 27%, respectively. The intestinal enzyme is shown to be of fetal origin, presumably arising from the direct entry of desquamated intestinal mucosal cells into the amniotic fluid. The bone/liver/kidney enzyme is mostly of maternal serum origin early with an increased fetal contribution toward term. Early in pregnancy, the placental ALP activity is probably derived both directly from the placenta and from the placental activity in maternal serum. The latter source contributes more toward term.", "contents": "Origin of the alkaline phosphatases in amniotic fluid. The nature and origins of amniotic fluid ALP activity were investigated early (14 to 22 weeks) and late (25 to 44 weeks) in pregnancy. The total enzyme activities for both stages were significantly different and considerable changes in the activities of individual enzyme components occurred. Early activity consists of intestinal (81%), bone/liver/kidney (15%), and placental (4%) ALP. In late fluids, the values are 5%, 69% and 27%, respectively. The intestinal enzyme is shown to be of fetal origin, presumably arising from the direct entry of desquamated intestinal mucosal cells into the amniotic fluid. The bone/liver/kidney enzyme is mostly of maternal serum origin early with an increased fetal contribution toward term. Early in pregnancy, the placental ALP activity is probably derived both directly from the placenta and from the placental activity in maternal serum. The latter source contributes more toward term."}
{"id": "PMID:474664", "title": "The effect of diet supplementation and addition of zinc in vitro on the growth-supporting property of amniotic fluid in African women.", "content": "The effect of diet supplementation throughout pregnancy on third-trimester amniotic fluid growth-supporting activity (GSP) was studied in 100 African women; 32 were given zinc supplementation, 22 each animal and vegetable supplements, respectively, and 24 served as control subjects. No difference in GSP was noted in any of the four groups, the majority of fluids (65%) being noninhibitory. Zinc levels in fluids from African women were much lower than those described for other population groups at corresponding periods of gestation. Although zinc levels in liquor rose following dietary zinc supplementation, these remained lower than values described in white patients. In vitro addition of zinc (up to 153 mu moles per liter final concentration) to 17 noninhibitory African liquors resulted in these fluids becoming inhibitory.", "contents": "The effect of diet supplementation and addition of zinc in vitro on the growth-supporting property of amniotic fluid in African women. The effect of diet supplementation throughout pregnancy on third-trimester amniotic fluid growth-supporting activity (GSP) was studied in 100 African women; 32 were given zinc supplementation, 22 each animal and vegetable supplements, respectively, and 24 served as control subjects. No difference in GSP was noted in any of the four groups, the majority of fluids (65%) being noninhibitory. Zinc levels in fluids from African women were much lower than those described for other population groups at corresponding periods of gestation. Although zinc levels in liquor rose following dietary zinc supplementation, these remained lower than values described in white patients. In vitro addition of zinc (up to 153 mu moles per liter final concentration) to 17 noninhibitory African liquors resulted in these fluids becoming inhibitory."}
{"id": "PMID:474665", "title": "Use of the minor hemoglobin ratio for the determination of gestational age.", "content": "Maternal and cord blood hemoglobin A1c were quantitated at the time of delivery by radioimmunoassay. Other cord blood hemoglobins were quantitated by column chromatography using Bio-Rex 70. The minor hemoglobin ratio : formula: (see text),showed an excellent linear correlation (r2  = 0.88) with gestational age. Therefore, the ratio of : formula: (see text), could be a useful test to determine gestational age in the third trimester.", "contents": "Use of the minor hemoglobin ratio for the determination of gestational age. Maternal and cord blood hemoglobin A1c were quantitated at the time of delivery by radioimmunoassay. Other cord blood hemoglobins were quantitated by column chromatography using Bio-Rex 70. The minor hemoglobin ratio : formula: (see text),showed an excellent linear correlation (r2  = 0.88) with gestational age. Therefore, the ratio of : formula: (see text), could be a useful test to determine gestational age in the third trimester."}
{"id": "PMID:474666", "title": "Activity and work during pregnancy and the postpartum period: a cross-cultural study of 202 societies.", "content": "Cross-cultural information on 202 traditional societies was perused for data on customs surrounding the expected work load of pregnant and postpartum women. The most common single pattern of work activity during pregnancy was that of continuing full duties until the onset of labor. A bare majority of societies did encourage a lightening of work at some time during pregnancy. Postnatally, most societies did restrict maternal work activity; however, few suspended usual work duties for prolonged periods of time. About one half of societies expected a return to full duties within 2 weeks. Results suggest that the current American trend toward increased participation of women of childbearing age in the work force may be in keeping with the work loads of women in traditional societies.", "contents": "Activity and work during pregnancy and the postpartum period: a cross-cultural study of 202 societies. Cross-cultural information on 202 traditional societies was perused for data on customs surrounding the expected work load of pregnant and postpartum women. The most common single pattern of work activity during pregnancy was that of continuing full duties until the onset of labor. A bare majority of societies did encourage a lightening of work at some time during pregnancy. Postnatally, most societies did restrict maternal work activity; however, few suspended usual work duties for prolonged periods of time. About one half of societies expected a return to full duties within 2 weeks. Results suggest that the current American trend toward increased participation of women of childbearing age in the work force may be in keeping with the work loads of women in traditional societies."}
{"id": "PMID:474667", "title": "Treating the emotional aspects of infertility: counseling services in an infertility clinic.", "content": "The physician treating the infertile couple, while attempting to diagnose and treat the cause of that problem, all too frequently overlooks the emotional aspects of involuntary sterility. Infertility can cause tremendous strains on the patients, either individually or as a couple. An approach to meeting these needs can be achieved through a program of counseling, provided as a service in an infertility center.", "contents": "Treating the emotional aspects of infertility: counseling services in an infertility clinic. The physician treating the infertile couple, while attempting to diagnose and treat the cause of that problem, all too frequently overlooks the emotional aspects of involuntary sterility. Infertility can cause tremendous strains on the patients, either individually or as a couple. An approach to meeting these needs can be achieved through a program of counseling, provided as a service in an infertility center."}
{"id": "PMID:474668", "title": "Anatomic and clinical correlates of uterine perforation.", "content": "We reviewed cases of uterine perforations which occurred at or were referred to the Boston Hospital for Women, Lying-In Division, over the 2 year period from mid-1975 to mid-1977. There were 25 uterine perforations; twenty patients were pregnant and five were not. In the pregnant patients, 16 perforations involved the cervix or the lower uterine segment, whereas only four were located in the uterine fundus; in the nonpregnant patients, all five were fundal perforations. In the pregnant patients, 12 required laparotomy, eight had serious lacerations of the uterine artery, and three had hysterectomy while none of the nonpregnant patients had lacerations or required subsequent procedures. Thus, there is a significant anatomic difference between those perforations which occurred in the pregnant patients and in the nonpregnant patients, the manner in which they presented clinically, and the need for intervention via laparotomy, subsequent morbidity, and outcome as reflected in future reproductive capability. Perforation at or near the cervix may be more common than previously assumed. Furthermore, two distinct clinical entities of cervical perforation exist in pregnant patients as based on the anatomic location of the defect. The anatomy of cervical perforations, their recognition, and their management are discussed.", "contents": "Anatomic and clinical correlates of uterine perforation. We reviewed cases of uterine perforations which occurred at or were referred to the Boston Hospital for Women, Lying-In Division, over the 2 year period from mid-1975 to mid-1977. There were 25 uterine perforations; twenty patients were pregnant and five were not. In the pregnant patients, 16 perforations involved the cervix or the lower uterine segment, whereas only four were located in the uterine fundus; in the nonpregnant patients, all five were fundal perforations. In the pregnant patients, 12 required laparotomy, eight had serious lacerations of the uterine artery, and three had hysterectomy while none of the nonpregnant patients had lacerations or required subsequent procedures. Thus, there is a significant anatomic difference between those perforations which occurred in the pregnant patients and in the nonpregnant patients, the manner in which they presented clinically, and the need for intervention via laparotomy, subsequent morbidity, and outcome as reflected in future reproductive capability. Perforation at or near the cervix may be more common than previously assumed. Furthermore, two distinct clinical entities of cervical perforation exist in pregnant patients as based on the anatomic location of the defect. The anatomy of cervical perforations, their recognition, and their management are discussed."}
{"id": "PMID:474669", "title": "Vulvar neoplasia in the young.", "content": "Vulvar intraepithelial neoplasms may be occurring more frequently among younger age groups. Six patients with carcinoma in situ or severe vulvar dysplasia are reported, as well as one patient with microinvasive carcinoma, all under the age of 30 years. The vulvar lesions were noted to be mostly multicentric. They were associated with other intraepithelial neoplasias of the cervix or vagina in four of the six cases. The lesions were most often mistaken for condyloma acuminatum. Histologic changes were primarily Bowen's disease or Bowenoid dysplasia.", "contents": "Vulvar neoplasia in the young. Vulvar intraepithelial neoplasms may be occurring more frequently among younger age groups. Six patients with carcinoma in situ or severe vulvar dysplasia are reported, as well as one patient with microinvasive carcinoma, all under the age of 30 years. The vulvar lesions were noted to be mostly multicentric. They were associated with other intraepithelial neoplasias of the cervix or vagina in four of the six cases. The lesions were most often mistaken for condyloma acuminatum. Histologic changes were primarily Bowen's disease or Bowenoid dysplasia."}
{"id": "PMID:474670", "title": "Acute pulmonary complications of molar pregnancy.", "content": "Of 128 patients receiving primary treatment for molar pregnancy at Women's Hospital, Los Angeles County/University of Southern California Medical Center, after uterine evacuation 12 (10.7%) developed self-limited, acute pulmonary complications characterized by tachycardia, tachypnea, and hypoxemia. Multiple contributing factors have been identified which include trophoblastic deportation, hyperthyroidism, fluid overload, dilutional anemia, and pre-eclampsia. While the final outcome was uniformly benign in our group, deaths have been reported. These patients are also at an increased risk for postmolar trophoblastic disease. The diagnosis and management of the postmolar pulmonary complications are discussed.", "contents": "Acute pulmonary complications of molar pregnancy. Of 128 patients receiving primary treatment for molar pregnancy at Women's Hospital, Los Angeles County/University of Southern California Medical Center, after uterine evacuation 12 (10.7%) developed self-limited, acute pulmonary complications characterized by tachycardia, tachypnea, and hypoxemia. Multiple contributing factors have been identified which include trophoblastic deportation, hyperthyroidism, fluid overload, dilutional anemia, and pre-eclampsia. While the final outcome was uniformly benign in our group, deaths have been reported. These patients are also at an increased risk for postmolar trophoblastic disease. The diagnosis and management of the postmolar pulmonary complications are discussed."}
{"id": "PMID:474671", "title": "Evaluation of a balloon dilator before second-trimester abortion by vacuum curettage.", "content": "We evaluated the cervical dilator device (CDD), an expanding balloon, as a substitute for laminaria tents before abortion at 13 to 16 1/2 weeks by extraction and vacuum curettage. The CDD was found to be an effective dilator, although it did not appear to have any advantage over laminaria tents when placed overnight and was associated with significant pain upon insertion and an apparent increased risk of endometritis. The present CDD or a subsequent modification may offer advantages over laminaria tents for short-term placement.", "contents": "Evaluation of a balloon dilator before second-trimester abortion by vacuum curettage. We evaluated the cervical dilator device (CDD), an expanding balloon, as a substitute for laminaria tents before abortion at 13 to 16 1/2 weeks by extraction and vacuum curettage. The CDD was found to be an effective dilator, although it did not appear to have any advantage over laminaria tents when placed overnight and was associated with significant pain upon insertion and an apparent increased risk of endometritis. The present CDD or a subsequent modification may offer advantages over laminaria tents for short-term placement."}
{"id": "PMID:474672", "title": "Vessel density in endometrium of women with and without intrauterine contraceptive devices: a morphometric evaluation.", "content": "Increased vascularity was found in the endometrial functionalis in uteri of women wearing intrauterine contraceptive devices (IUDs) as compared to uteri of women without IUDs (control subjects). Vessel concentration was highest in endometrial tissue adjacent to that tissue which was depressed by the IUD. In control tissues there was a significant variation in vascularity according to geographic location in the following order of magnitude: fundus greater than corpus greater than cornua greater than isthmus. No significant variation was found, however, among different phases of the ovarian cycle in either control or IUD cases. Increased endometrial vascularity could be a reaction to vessel damage caused by the IUD and for several reasons may contribute to IUD-induced endometrial bleeding.", "contents": "Vessel density in endometrium of women with and without intrauterine contraceptive devices: a morphometric evaluation. Increased vascularity was found in the endometrial functionalis in uteri of women wearing intrauterine contraceptive devices (IUDs) as compared to uteri of women without IUDs (control subjects). Vessel concentration was highest in endometrial tissue adjacent to that tissue which was depressed by the IUD. In control tissues there was a significant variation in vascularity according to geographic location in the following order of magnitude: fundus greater than corpus greater than cornua greater than isthmus. No significant variation was found, however, among different phases of the ovarian cycle in either control or IUD cases. Increased endometrial vascularity could be a reaction to vessel damage caused by the IUD and for several reasons may contribute to IUD-induced endometrial bleeding."}
{"id": "PMID:474674", "title": "Corticosteroid-binding globulin and estrogens in maternal and cord blood.", "content": "Corticosteroid-binding globulin (CBG) is produced by the liver in small concentrations and binds steroids with high affinity. Its concentration in the blood is sensitive to endogenous or exogenous estrogens in a dose-response manner and serves as a biological assay for estrogens. CBG concentrations were measured in sera collected from maternal and umbilical vein blood during labor and delivery and at elective cesarean section and correlated with endogenous estradiol and estriol concentrations. CBG and estradiol concentrations of maternal blood were significantly exceeded those of maternal serum. A highly significant increase in cord blood estriol concentration was found following vaginal delivery compared to elective cesarean section, indicating an increase in adrenal activity due to the stress of labor. CBG levels correlated directly with maternal estradiol but not estriol cencentrations. Cord blood CBG concentration varied little despite a wide range of estrogen concentrations. The physiologic significance of CBG concentrations in maternal and fetal blood is discussed.", "contents": "Corticosteroid-binding globulin and estrogens in maternal and cord blood. Corticosteroid-binding globulin (CBG) is produced by the liver in small concentrations and binds steroids with high affinity. Its concentration in the blood is sensitive to endogenous or exogenous estrogens in a dose-response manner and serves as a biological assay for estrogens. CBG concentrations were measured in sera collected from maternal and umbilical vein blood during labor and delivery and at elective cesarean section and correlated with endogenous estradiol and estriol concentrations. CBG and estradiol concentrations of maternal blood were significantly exceeded those of maternal serum. A highly significant increase in cord blood estriol concentration was found following vaginal delivery compared to elective cesarean section, indicating an increase in adrenal activity due to the stress of labor. CBG levels correlated directly with maternal estradiol but not estriol cencentrations. Cord blood CBG concentration varied little despite a wide range of estrogen concentrations. The physiologic significance of CBG concentrations in maternal and fetal blood is discussed."}
{"id": "PMID:474673", "title": "Computer analysis of etiology and pregnancy rate in 636 cases of primary infertility.", "content": "Since the rate of pregnancy is a function of time, conventional pregnancy rates (number of patients achieving pregnancy per number of patients treated) are inadequate for counseling unless the follow-up period is specified. To overcome this problem, the expectancy of pregnancy for 636 cases of primary infertility was calculated with the assumption that the patients were followed up indefinitely. The overall \"conventional\" pregnancy rate was 38%, whereas the overall expectancy of pregnancy was 64%. Endometriosis was found to be the most common factor, comprising 25% of the cases, with a pregnancy rate of 31% and an expectancy of 52%. The expectancy of future pregnancy in a patient who has not achieved pregnancy by a given time is presented for each etiologic factor. This paper also presents a comparison of expectancies of pregnancy by different treatments, which may be helpful in selecting appropriate therapy.", "contents": "Computer analysis of etiology and pregnancy rate in 636 cases of primary infertility. Since the rate of pregnancy is a function of time, conventional pregnancy rates (number of patients achieving pregnancy per number of patients treated) are inadequate for counseling unless the follow-up period is specified. To overcome this problem, the expectancy of pregnancy for 636 cases of primary infertility was calculated with the assumption that the patients were followed up indefinitely. The overall \"conventional\" pregnancy rate was 38%, whereas the overall expectancy of pregnancy was 64%. Endometriosis was found to be the most common factor, comprising 25% of the cases, with a pregnancy rate of 31% and an expectancy of 52%. The expectancy of future pregnancy in a patient who has not achieved pregnancy by a given time is presented for each etiologic factor. This paper also presents a comparison of expectancies of pregnancy by different treatments, which may be helpful in selecting appropriate therapy."}
{"id": "PMID:474675", "title": "Cadmium uptake by the rat embryo as a function of gestational age.", "content": "Maternal blood, liver, kidney, and placental and fetal (embryo) accumulation of cadmium (Cd), a known embryotoxic trace element, was investigated following a single oral dose of various amounts of Cd (10 to 1,000 microgram/rat) as CdCl2 containing 109Cd at days 6, 10, 14, and 17 of gestation. Twenty-four hours after Cd administration the rats were killed and the various tissues were counted in a gamma well counter for determination of 109Cd activity. Maternal liver and kidneys were the main target organs of Cd accumulation at all stages of gestation. Embryo levels of Cd were highest prior to formation of the functional placenta. After placental formation, fetal Cd levels were decreased, while placental accumulation of Cd increased with increasing gestational age. The results indicate that the embryo accumulates the greatest percentage of ingested Cd between implantation and placentation, the early period of organogenesis. The placenta apparently protects the fetus from exposure to this element during the last third of gestation.", "contents": "Cadmium uptake by the rat embryo as a function of gestational age. Maternal blood, liver, kidney, and placental and fetal (embryo) accumulation of cadmium (Cd), a known embryotoxic trace element, was investigated following a single oral dose of various amounts of Cd (10 to 1,000 microgram/rat) as CdCl2 containing 109Cd at days 6, 10, 14, and 17 of gestation. Twenty-four hours after Cd administration the rats were killed and the various tissues were counted in a gamma well counter for determination of 109Cd activity. Maternal liver and kidneys were the main target organs of Cd accumulation at all stages of gestation. Embryo levels of Cd were highest prior to formation of the functional placenta. After placental formation, fetal Cd levels were decreased, while placental accumulation of Cd increased with increasing gestational age. The results indicate that the embryo accumulates the greatest percentage of ingested Cd between implantation and placentation, the early period of organogenesis. The placenta apparently protects the fetus from exposure to this element during the last third of gestation."}
{"id": "PMID:474676", "title": "Changes in 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D during pregnancy.", "content": "Serum concentrations of 25-hydroxyvitamin D (25OHD) and 24,25-dihydroxyvitamin D (24,25(OH)2D) were measured in a cross-sectional study of 94 normal pregnant women at various stages of gestation in order to assess the hormonal regulation of calcium homeostasis during gestation. The 40 week gestational period was divided into four 10 weeks quarters. 25OHD concentration were significantly below control levels (32.2 +/- 3.1 (S.E.) ng/ml) by the second quarter of pregnancy and were even lower at term. Serum levels of 24,25(OH)2D did not decrease until the fourth quarter, when the mean concentration (0.8 +/- 0.1 ng/ml) was approximately one half the control values (1.5 +/- 0.3, p less than 0.025). These data suggest that the metabolic pathways of vitamin D are altered during gestation, perhaps in response to increasing mother-to-fetus transport of calcium. There is decreased 24-hydroxylation and, in view of the lowered 25OHD levels, possibly increased production of 1,25-hydroxyvitamin D (1,25(OH)2D).", "contents": "Changes in 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D during pregnancy. Serum concentrations of 25-hydroxyvitamin D (25OHD) and 24,25-dihydroxyvitamin D (24,25(OH)2D) were measured in a cross-sectional study of 94 normal pregnant women at various stages of gestation in order to assess the hormonal regulation of calcium homeostasis during gestation. The 40 week gestational period was divided into four 10 weeks quarters. 25OHD concentration were significantly below control levels (32.2 +/- 3.1 (S.E.) ng/ml) by the second quarter of pregnancy and were even lower at term. Serum levels of 24,25(OH)2D did not decrease until the fourth quarter, when the mean concentration (0.8 +/- 0.1 ng/ml) was approximately one half the control values (1.5 +/- 0.3, p less than 0.025). These data suggest that the metabolic pathways of vitamin D are altered during gestation, perhaps in response to increasing mother-to-fetus transport of calcium. There is decreased 24-hydroxylation and, in view of the lowered 25OHD levels, possibly increased production of 1,25-hydroxyvitamin D (1,25(OH)2D)."}
{"id": "PMID:474677", "title": "1977 National survey of support in space and dollars for departments of obstetrics and gynecology from their medical schools.", "content": "A questionnaire was sent to all chairpersons in obstetrics and gynecology departments in United States Medical Schools in the fall of 1977 seeking information on the school's support of that department for the year 1977 in terms of space and dollar commitments. A total of 113 schools were contacted; 106 responded (93.8%) and the data were tabulated. There were no significant differences between the private and state school resources. The average space commitment was 9,277 +/- SEM 709 square feet and the average school budget was 441,023 +/- SEM 24,265 dollars/year. These data were adjusted for class size and provide the first data base for assessing the adequacy of a medical school's commitment to a department of obstetrics and gynecology.", "contents": "1977 National survey of support in space and dollars for departments of obstetrics and gynecology from their medical schools. A questionnaire was sent to all chairpersons in obstetrics and gynecology departments in United States Medical Schools in the fall of 1977 seeking information on the school's support of that department for the year 1977 in terms of space and dollar commitments. A total of 113 schools were contacted; 106 responded (93.8%) and the data were tabulated. There were no significant differences between the private and state school resources. The average space commitment was 9,277 +/- SEM 709 square feet and the average school budget was 441,023 +/- SEM 24,265 dollars/year. These data were adjusted for class size and provide the first data base for assessing the adequacy of a medical school's commitment to a department of obstetrics and gynecology."}
{"id": "PMID:474678", "title": "The impact of midtrimester abortion techniques on patients and staff.", "content": "We studied 250 midtrimester abortions by dilatation and extraction (D and E) under general anesthesia and compared them with abortions by the intra-amniotic injection of prostaglandin (amnio) in order to assess the physical and emotional changes experienced by patients and staff under the different circumstances represented by each procedure. We found patients undergoing D and E abortions had fewer physical complications and described the procedure as minor surgery which went smoothly. The patients who had amnio abortions had more pain and reacted with more anger and depression afterward. Nurses were more disturbed by amnio abortions in which they played major roles in supporting the patient as well as in her abortion. Physicians reported the D and E procedures to be emotionally difficult which may limit the adoption of this procedure despite its value to patients.", "contents": "The impact of midtrimester abortion techniques on patients and staff. We studied 250 midtrimester abortions by dilatation and extraction (D and E) under general anesthesia and compared them with abortions by the intra-amniotic injection of prostaglandin (amnio) in order to assess the physical and emotional changes experienced by patients and staff under the different circumstances represented by each procedure. We found patients undergoing D and E abortions had fewer physical complications and described the procedure as minor surgery which went smoothly. The patients who had amnio abortions had more pain and reacted with more anger and depression afterward. Nurses were more disturbed by amnio abortions in which they played major roles in supporting the patient as well as in her abortion. Physicians reported the D and E procedures to be emotionally difficult which may limit the adoption of this procedure despite its value to patients."}
{"id": "PMID:474679", "title": "The evaluation of uterine activity: a comparative analysis.", "content": "In this study a comparison is made between the most widely accepted method of expressing uterine activity, Montevideo units, and an automated on-line method expressed as uterine activity units. Although the actual numerical expression differs, the percentage of variation when the two values are compared is small at various phases of labor. The variation between the methods for uterine activity required to progress from 4 to 6 cm cervical dilatation and from 6 to 10 cm cervical dilatation is less than 5%. Both methods closely agree on the actual expression of uterine activity or uterine work.", "contents": "The evaluation of uterine activity: a comparative analysis. In this study a comparison is made between the most widely accepted method of expressing uterine activity, Montevideo units, and an automated on-line method expressed as uterine activity units. Although the actual numerical expression differs, the percentage of variation when the two values are compared is small at various phases of labor. The variation between the methods for uterine activity required to progress from 4 to 6 cm cervical dilatation and from 6 to 10 cm cervical dilatation is less than 5%. Both methods closely agree on the actual expression of uterine activity or uterine work."}
{"id": "PMID:474687", "title": "Effect of local anesthetics on intraocular penetration of subconjunctival antibiotics.", "content": "The combination of the subconjunctival injection of lidocaine HCl or procaine HCl with the subconjunctival injection of penicillin G, methicillin, cephaloridine, or gentamicin did not influence their intraocular penetration or subsequent antibiotic activity in aqueous humor of experimental animals.", "contents": "Effect of local anesthetics on intraocular penetration of subconjunctival antibiotics. The combination of the subconjunctival injection of lidocaine HCl or procaine HCl with the subconjunctival injection of penicillin G, methicillin, cephaloridine, or gentamicin did not influence their intraocular penetration or subsequent antibiotic activity in aqueous humor of experimental animals."}
{"id": "PMID:474688", "title": "Scanning electron microscopy of congenital corneal leukomas (Peters' anomaly).", "content": "Specimens of three corneas in two patients with Peter's anomaly were obtained at the time of penetrating keratoplasty and studied by scanning and transmission electron microscopy. In one patient, the anomaly was monocular, and the endothelial surface showed a central defect in Descemet's layer with isolated rounded defects in the midperiphery. Fine collagenous material covered the posterior surface. The other two specimens were obtained from a patient with rubella syndrome without cataracts. The cornea showed malformation of Descemet's membrane with fibroblastic overgrowth on the endothelial layer. Epithelial-like cells and leukocytes were also found. The congenital central leukoma we believe was caused by adhesion of the pupillary membrane in our first patient, and possibly was inflammatory in our second patient.", "contents": "Scanning electron microscopy of congenital corneal leukomas (Peters' anomaly). Specimens of three corneas in two patients with Peter's anomaly were obtained at the time of penetrating keratoplasty and studied by scanning and transmission electron microscopy. In one patient, the anomaly was monocular, and the endothelial surface showed a central defect in Descemet's layer with isolated rounded defects in the midperiphery. Fine collagenous material covered the posterior surface. The other two specimens were obtained from a patient with rubella syndrome without cataracts. The cornea showed malformation of Descemet's membrane with fibroblastic overgrowth on the endothelial layer. Epithelial-like cells and leukocytes were also found. The congenital central leukoma we believe was caused by adhesion of the pupillary membrane in our first patient, and possibly was inflammatory in our second patient."}
{"id": "PMID:474689", "title": "Treatment of Thygeson's superficial punctate keratopathy with soft contact lenses.", "content": "Three patients had Thygeson's superficial punctate keratopathy; two of whom previously had required prolonged topical corticosteroid therapy for symptomatic relief. The use of therapeutic soft contact lenses provided improved visual acuity as well as symptomatic relief in all three patients. Corneal opacities faded while the soft contact lenses were in place. One patient who had been on long-term topical corticosteroids was gradually taken off this medication with the use of soft contact lens. Another patient noted only mild symptoms from acute episodes while wearing soft contact lenses; only minimal topical corticosteroids were required to control her symptoms. Two additional patients were treated with soft contact lenses. One could not tolerate soft lens wear and the other chose corticosteroid therapy instead of soft lens wear. Soft contact lenses are an acceptable alternative to topical corticosteroid therapy in the treatment of Thygeson's superficial punctate keratopathy.", "contents": "Treatment of Thygeson's superficial punctate keratopathy with soft contact lenses. Three patients had Thygeson's superficial punctate keratopathy; two of whom previously had required prolonged topical corticosteroid therapy for symptomatic relief. The use of therapeutic soft contact lenses provided improved visual acuity as well as symptomatic relief in all three patients. Corneal opacities faded while the soft contact lenses were in place. One patient who had been on long-term topical corticosteroids was gradually taken off this medication with the use of soft contact lens. Another patient noted only mild symptoms from acute episodes while wearing soft contact lenses; only minimal topical corticosteroids were required to control her symptoms. Two additional patients were treated with soft contact lenses. One could not tolerate soft lens wear and the other chose corticosteroid therapy instead of soft lens wear. Soft contact lenses are an acceptable alternative to topical corticosteroid therapy in the treatment of Thygeson's superficial punctate keratopathy."}
{"id": "PMID:474690", "title": "Results of keratoprosthesis.", "content": "The technique of keratoprosthesis was  used in 21 cases of severe and intractable forms of keratopathy during the past 3 1/2 years. The results in terms of visual acuity were satisfactory in more than half the cases. Significant problems that occurred after this procedure included the diagnosis and management of glaucoma, vitreitis, and retroprosthetic membrane. There were no instances of extrusion of the keratoprosthesis nor were there any cases of endophthalmitis. However, one case developed necrosis of the tissue around the optical cylinder on the eyelid after surgery. Although its use is limited to only some forms of keratopathy, keratoprosthesis seems to offer a solution in cases where no other treatment is available.", "contents": "Results of keratoprosthesis. The technique of keratoprosthesis was  used in 21 cases of severe and intractable forms of keratopathy during the past 3 1/2 years. The results in terms of visual acuity were satisfactory in more than half the cases. Significant problems that occurred after this procedure included the diagnosis and management of glaucoma, vitreitis, and retroprosthetic membrane. There were no instances of extrusion of the keratoprosthesis nor were there any cases of endophthalmitis. However, one case developed necrosis of the tissue around the optical cylinder on the eyelid after surgery. Although its use is limited to only some forms of keratopathy, keratoprosthesis seems to offer a solution in cases where no other treatment is available."}
{"id": "PMID:474691", "title": "A comparison of diagnostic techniques in angle-closure glaucoma.", "content": "We evaluated four diagnostic techniques for angle-closure glaucoma (Koeppe gonioscopy, Goldmann three-mirror gonioscopy, compressive Zeiss four-mirror gonioscopy, and the operative chamber deepening procedure) to ascertain their relative effectiveness in determining: (1) whether the angle was open or closed, and (2) whether the closure was appositional or synechial and permanent. Koeppe gonioscopy was the most reliable way to determine whether the angle was open or closed, because this lens caused no artifactual widening of the peripheral angle and allowed the best view over an often convex iris. Zeiss four-mirror compressive gonioscopy or the operative chamber deepening procedure were equally reliable ways to determine whether the closure was appositional or synechial and permanent. A comparison between compressive Zeiss four-mirror gonioscopy and the operative chamber deepening procedure revealed that the latter has therapeutic value as well as diagnostic value, that is, it can separate recently formed adhesions.", "contents": "A comparison of diagnostic techniques in angle-closure glaucoma. We evaluated four diagnostic techniques for angle-closure glaucoma (Koeppe gonioscopy, Goldmann three-mirror gonioscopy, compressive Zeiss four-mirror gonioscopy, and the operative chamber deepening procedure) to ascertain their relative effectiveness in determining: (1) whether the angle was open or closed, and (2) whether the closure was appositional or synechial and permanent. Koeppe gonioscopy was the most reliable way to determine whether the angle was open or closed, because this lens caused no artifactual widening of the peripheral angle and allowed the best view over an often convex iris. Zeiss four-mirror compressive gonioscopy or the operative chamber deepening procedure were equally reliable ways to determine whether the closure was appositional or synechial and permanent. A comparison between compressive Zeiss four-mirror gonioscopy and the operative chamber deepening procedure revealed that the latter has therapeutic value as well as diagnostic value, that is, it can separate recently formed adhesions."}
{"id": "PMID:474692", "title": "Abnormalities of central contrast sensitivity in glaucoma.", "content": "The detectability of foveally presented low-contrast flickering stimuli was determined for glaucoma patients, ocular hypertensives, and normal control subjects. Two types of stimuli, a homogeneous flickering field, and a counterphase flickering grating of low spatial frequency, were presented on a screen subtending 4 degrees of visual angle. The average of the contrast sensitivities to these two simuli (defined as the dynamic response coefficient) was consistently lower in glaucomatous than in normotensive eyes. The dynamic response coefficient was also below normal in half the ocular hypertensive eyes.", "contents": "Abnormalities of central contrast sensitivity in glaucoma. The detectability of foveally presented low-contrast flickering stimuli was determined for glaucoma patients, ocular hypertensives, and normal control subjects. Two types of stimuli, a homogeneous flickering field, and a counterphase flickering grating of low spatial frequency, were presented on a screen subtending 4 degrees of visual angle. The average of the contrast sensitivities to these two simuli (defined as the dynamic response coefficient) was consistently lower in glaucomatous than in normotensive eyes. The dynamic response coefficient was also below normal in half the ocular hypertensive eyes."}
{"id": "PMID:474693", "title": "HLA antigens and acute angle-closure glaucoma.", "content": "Thirty-five unrelated white patients with acute angle-closure glaucoma and 126 previously healthy unrelated white cadaveric kidney donors were typed for histocompatibility (HLA) antigens. Upon Z-ratio proportionality testing, no association between acute angle closure glaucoma and any specific HLA antigen was noted.", "contents": "HLA antigens and acute angle-closure glaucoma. Thirty-five unrelated white patients with acute angle-closure glaucoma and 126 previously healthy unrelated white cadaveric kidney donors were typed for histocompatibility (HLA) antigens. Upon Z-ratio proportionality testing, no association between acute angle closure glaucoma and any specific HLA antigen was noted."}
{"id": "PMID:474694", "title": "Anterior chamber depth measurement using the split-lamp microscope.", "content": "I devised a new and simple method to measure the anterior chamber depth that requires only a Haag-Streit 900 slit lamp. Viewing the anterior chamber at 45 degrees and matching the height of the corneal slit beam to the depth of the anterior chamber enables the observer to calculate the anterior chamber depth by multiplying the measured slit height by 1.4 or, more accurately, by use of the table provided. The accuracy over the range of clinically encountered depths of the anterior changer is +/- 0.2 mm relative to standard pachometry.", "contents": "Anterior chamber depth measurement using the split-lamp microscope. I devised a new and simple method to measure the anterior chamber depth that requires only a Haag-Streit 900 slit lamp. Viewing the anterior chamber at 45 degrees and matching the height of the corneal slit beam to the depth of the anterior chamber enables the observer to calculate the anterior chamber depth by multiplying the measured slit height by 1.4 or, more accurately, by use of the table provided. The accuracy over the range of clinically encountered depths of the anterior changer is +/- 0.2 mm relative to standard pachometry."}
{"id": "PMID:474695", "title": "The effects of intravitreal irrigation during vitrectomy on the electroretinogram.", "content": "Intravitreal irrigation with Balanced Salt Solution Plus (BSS Plus) produced less decrease in b-wave amplitude than either normal saline solution or Balanced Salt Solution. BSS Plus was more suitable for intravitreal irrigation because it contained the appropriate bicarbonate, pH, and ionic composition necessary for maintenance of normal retinal electrical activity, and it contained glutathione, which is necessary for maintenance of endothelial cell adenosine triphosphatase and for protection against free radical damage and oxidative stress.", "contents": "The effects of intravitreal irrigation during vitrectomy on the electroretinogram. Intravitreal irrigation with Balanced Salt Solution Plus (BSS Plus) produced less decrease in b-wave amplitude than either normal saline solution or Balanced Salt Solution. BSS Plus was more suitable for intravitreal irrigation because it contained the appropriate bicarbonate, pH, and ionic composition necessary for maintenance of normal retinal electrical activity, and it contained glutathione, which is necessary for maintenance of endothelial cell adenosine triphosphatase and for protection against free radical damage and oxidative stress."}
{"id": "PMID:474696", "title": "Clinical results in vitrectomy for diabetic traction retinal detachment.", "content": "A series of 75 eyes underwent pars plana vitrectomy for progressive diabetic traction retinal detachment of the macula. In all cases, the media was sufficiently clear to allow preoperative macular examination. The overall visual improvement was higher in phakic eyes (80%) than aphakic eyes (72%) and the rate of neovascular glaucoma was less in phakic eyes. The difference was not statistically significant, however. The final visual acuity corresponded to the degree of preoperative visual deterioration. Thus, surgery during the rapidly evolving proliferative and retracting process did not adversely affect the visual success rate when compared to previous reports on longstanding vitreous hemorrhage.", "contents": "Clinical results in vitrectomy for diabetic traction retinal detachment. A series of 75 eyes underwent pars plana vitrectomy for progressive diabetic traction retinal detachment of the macula. In all cases, the media was sufficiently clear to allow preoperative macular examination. The overall visual improvement was higher in phakic eyes (80%) than aphakic eyes (72%) and the rate of neovascular glaucoma was less in phakic eyes. The difference was not statistically significant, however. The final visual acuity corresponded to the degree of preoperative visual deterioration. Thus, surgery during the rapidly evolving proliferative and retracting process did not adversely affect the visual success rate when compared to previous reports on longstanding vitreous hemorrhage."}
{"id": "PMID:474697", "title": "Orbital hemorrhage.", "content": "Seventeen patients had spontaneous orbital hemorrhages. The usual symptoms were acute onset of pain, proptosis, and vomiting with decreased vision, limitation of motility, and ecchymosis of the eyelids occurring in some patients. The children often developed a progressive space occupying lesion that simulated a neoplasm. Most patients had underlying venous anomalies, although several elderly patients with atherosclerosis developed arterial hemorrhages with more abrupt and dramatic symptoms. Other associated conditions included hypertension, anemia, labor, and von Willebrand's disease. The visual outcome was good except in the elderly patients, half of whom had severe and permanent visual loss.", "contents": "Orbital hemorrhage. Seventeen patients had spontaneous orbital hemorrhages. The usual symptoms were acute onset of pain, proptosis, and vomiting with decreased vision, limitation of motility, and ecchymosis of the eyelids occurring in some patients. The children often developed a progressive space occupying lesion that simulated a neoplasm. Most patients had underlying venous anomalies, although several elderly patients with atherosclerosis developed arterial hemorrhages with more abrupt and dramatic symptoms. Other associated conditions included hypertension, anemia, labor, and von Willebrand's disease. The visual outcome was good except in the elderly patients, half of whom had severe and permanent visual loss."}
{"id": "PMID:474698", "title": "A simulator for teaching visual field techniques on Goldmann or Goldmann-type perimeters.", "content": "We developed a visual field simulator for teaching perimetry on a Goldmann-type perimeter. It can present reproducible normal and defective visual fields, is portable and can be used without being attached to the perimeter, and will provide an easier way to train individuals in the correct techniques of perimetry.", "contents": "A simulator for teaching visual field techniques on Goldmann or Goldmann-type perimeters. We developed a visual field simulator for teaching perimetry on a Goldmann-type perimeter. It can present reproducible normal and defective visual fields, is portable and can be used without being attached to the perimeter, and will provide an easier way to train individuals in the correct techniques of perimetry."}
{"id": "PMID:474699", "title": "A system for counting endothelial cells from transparencies and negatives.", "content": "We have developed a system for endothelial cell density calculation and morphologic examination, which has proven to be convenient and reproducible, by using a currently available viewing unit, a few inexpensive materials, and simple arithmetic.", "contents": "A system for counting endothelial cells from transparencies and negatives. We have developed a system for endothelial cell density calculation and morphologic examination, which has proven to be convenient and reproducible, by using a currently available viewing unit, a few inexpensive materials, and simple arithmetic."}
{"id": "PMID:474700", "title": "Nontraumatic rupture of the globe secondary to orbital cellulitis.", "content": "A 53-year-old woman had a spontaneous nontraumatic rupture of the globe secondary to severely increased orbital pressure from an orbital cellulitis. To our knowledge this is the first such case reported.", "contents": "Nontraumatic rupture of the globe secondary to orbital cellulitis. A 53-year-old woman had a spontaneous nontraumatic rupture of the globe secondary to severely increased orbital pressure from an orbital cellulitis. To our knowledge this is the first such case reported."}
{"id": "PMID:474705", "title": "Increased myocardial catalase in rats fed ethanol.", "content": "The effects of chronic intake of dietary ethanol upon catalase, an enzyme capable of metabolizing ethanol, as well as upon myocardial morphology and hemodynamics, were studied in the rat. Ethanol, comprising 36% of dietary calories, administered to rats for 5 weeks, was associated with increased myocardial catalase of 45.9 +/- 3.7 IU/mg protein, compared to 21.0 +/- 1.8 IU/mg protein in pair-fed controls. The enzyme activity remained significantly elevated after 18 weeks of ethanol. Hepatic catalase did not differ in these groups. Parallel cytochemical studies confirmed the increase in myocardial catalase by demonstrating an increase in peroxisomes. Gross and light-microscopic examinations revealed no abnormalities at either 5 or 18 weeks. Remarkably few ultrastructural abnormalities were seen in this material fixed by vascular perfusion. Hemodynamic studies after 5 weeks of ethanol revealed decreased left ventricle systolic pressure and decreased mean arterial pressure but no change in ventricular filling pressure. The possibility of catalase playing a metabolic and potentially protective role in rat myocardium chronically exposed to ethanol is discussed.", "contents": "Increased myocardial catalase in rats fed ethanol. The effects of chronic intake of dietary ethanol upon catalase, an enzyme capable of metabolizing ethanol, as well as upon myocardial morphology and hemodynamics, were studied in the rat. Ethanol, comprising 36% of dietary calories, administered to rats for 5 weeks, was associated with increased myocardial catalase of 45.9 +/- 3.7 IU/mg protein, compared to 21.0 +/- 1.8 IU/mg protein in pair-fed controls. The enzyme activity remained significantly elevated after 18 weeks of ethanol. Hepatic catalase did not differ in these groups. Parallel cytochemical studies confirmed the increase in myocardial catalase by demonstrating an increase in peroxisomes. Gross and light-microscopic examinations revealed no abnormalities at either 5 or 18 weeks. Remarkably few ultrastructural abnormalities were seen in this material fixed by vascular perfusion. Hemodynamic studies after 5 weeks of ethanol revealed decreased left ventricle systolic pressure and decreased mean arterial pressure but no change in ventricular filling pressure. The possibility of catalase playing a metabolic and potentially protective role in rat myocardium chronically exposed to ethanol is discussed."}
{"id": "PMID:474706", "title": "Endocrine cells in the intestinal metaplasia of gastric mucosa.", "content": "Sections of gastric mucosa removed during surgery for cancer or peptic ulcer and containing regions of intestinal metaplasia were studied by the immunofluorescence technique using several antiserums against intestinal hormones. Endocrine cells such as cells containing somatostatin, glicentin (gut GLI-I), motilin, and probably cholecystokinin were found within metaplastic intestinal epithelium while secretin and neurotensin, which are present in the normal intestinal mucosa, were not detected in metaplastic epithelium. The endocrine-cell population present in the intestinal metaplasia resembles that found in the cryptal region of the normal small intestine, a finding in accordance with the fact that intestinal metaplasia of gastric mucosa usually reproduces structural and histochemical characteristics of small intestinal crypts.", "contents": "Endocrine cells in the intestinal metaplasia of gastric mucosa. Sections of gastric mucosa removed during surgery for cancer or peptic ulcer and containing regions of intestinal metaplasia were studied by the immunofluorescence technique using several antiserums against intestinal hormones. Endocrine cells such as cells containing somatostatin, glicentin (gut GLI-I), motilin, and probably cholecystokinin were found within metaplastic intestinal epithelium while secretin and neurotensin, which are present in the normal intestinal mucosa, were not detected in metaplastic epithelium. The endocrine-cell population present in the intestinal metaplasia resembles that found in the cryptal region of the normal small intestine, a finding in accordance with the fact that intestinal metaplasia of gastric mucosa usually reproduces structural and histochemical characteristics of small intestinal crypts."}
{"id": "PMID:474707", "title": "Degenerative vascular disease and myocardial infarction in mice with lupus-like syndrome.", "content": "The pathogenesis of the degenerative vascular disease and myocardial infarction that develop in mice with lupus-like disease was studied by immunofluorescence, light microscopy, and electron microscopy. Medium and small coronary arteries and arterioles of both infarcted and noninfarcted hearts had focal degenerative lesions consisting of deposits of periodic-acid--Schiff (PAS)-positive or eosinophilic material in the intima and to a lesser extent in the media, degenerative changes in the media without accompanying cellular inflammation, and occasional proliferation or swelling of intimal cells. These lesions often narrowed and, together with platelet aggregation, occasionally occluded the vascular lumens. Granular deposits of mouse immunoglobulin, C3, and occasionally gp70 were present in the walls of medium and small arteries, arterioles, and venules of both infarcted and noninfarcted myocardium. Dense deposits of foreign material were found by electron microscopy in areas corresponding to the immune deposits. These findings are consistent with the interpretation that these noninflammatory vascular lesions are caused by local deposition of antigen--antibody complexes. The immune-complex--mediated injury appears to lead to thrombotic and/or obliterative vascular changes that contribute to decrease of the coronary blood flow and to the development of myocardial infarction.", "contents": "Degenerative vascular disease and myocardial infarction in mice with lupus-like syndrome. The pathogenesis of the degenerative vascular disease and myocardial infarction that develop in mice with lupus-like disease was studied by immunofluorescence, light microscopy, and electron microscopy. Medium and small coronary arteries and arterioles of both infarcted and noninfarcted hearts had focal degenerative lesions consisting of deposits of periodic-acid--Schiff (PAS)-positive or eosinophilic material in the intima and to a lesser extent in the media, degenerative changes in the media without accompanying cellular inflammation, and occasional proliferation or swelling of intimal cells. These lesions often narrowed and, together with platelet aggregation, occasionally occluded the vascular lumens. Granular deposits of mouse immunoglobulin, C3, and occasionally gp70 were present in the walls of medium and small arteries, arterioles, and venules of both infarcted and noninfarcted myocardium. Dense deposits of foreign material were found by electron microscopy in areas corresponding to the immune deposits. These findings are consistent with the interpretation that these noninflammatory vascular lesions are caused by local deposition of antigen--antibody complexes. The immune-complex--mediated injury appears to lead to thrombotic and/or obliterative vascular changes that contribute to decrease of the coronary blood flow and to the development of myocardial infarction."}
{"id": "PMID:474708", "title": "Eosinophilic fasciitis. A pathologic study of twenty cases.", "content": "This report presents a detailed light-microscopic evaluation of biopsies obtained from 20 patients with eosinophilic fasciitis, a newly recognized disorder characterized by inflammation and thickening of the deep fascia, hypergammaglobulinemia, and peripheral and tissue eosinophilia. Early in the course of the disease, the deep fascia and lower subcutis are edematous and infiltrated with lymphocytes, plasma cells, histiocytes, and eosinophils; these features are associated with impressive peripheral eosinophilia. As the illness progresses, these structures and eventually the dermis become collagenized, thickened, and sclerotic. Tissue eosinophilia may be focal or diffuse and is usually observed in the fascia and/or lower subcutis. Extracutaneous involvement has been limited to a chronic synovitis and tenosynovitis, the latter frequently associated with the carpal tunnel syndrome. Deposits of immunoglobulin and/or complement were found in five of eight biopsies studied by direct immunofluorescence, which suggests that an immunologic stimulus may be responsible for initiating this syndrome. Differential diagnoses are discussed.", "contents": "Eosinophilic fasciitis. A pathologic study of twenty cases. This report presents a detailed light-microscopic evaluation of biopsies obtained from 20 patients with eosinophilic fasciitis, a newly recognized disorder characterized by inflammation and thickening of the deep fascia, hypergammaglobulinemia, and peripheral and tissue eosinophilia. Early in the course of the disease, the deep fascia and lower subcutis are edematous and infiltrated with lymphocytes, plasma cells, histiocytes, and eosinophils; these features are associated with impressive peripheral eosinophilia. As the illness progresses, these structures and eventually the dermis become collagenized, thickened, and sclerotic. Tissue eosinophilia may be focal or diffuse and is usually observed in the fascia and/or lower subcutis. Extracutaneous involvement has been limited to a chronic synovitis and tenosynovitis, the latter frequently associated with the carpal tunnel syndrome. Deposits of immunoglobulin and/or complement were found in five of eight biopsies studied by direct immunofluorescence, which suggests that an immunologic stimulus may be responsible for initiating this syndrome. Differential diagnoses are discussed."}
{"id": "PMID:474709", "title": "Metaplastic and mitotic activity of the ischemic (endocrine) kidney in experimental renal hypertension.", "content": "Partial ligation of the aorta between the renal arteries in the rat induces malignant hypertension, metaplasia of smooth-muscle cells of arterioles and arteries into juxtaglomerular cells, and a complex series of events in tubular cells at all levels of the ischemic kidney. The tubular cells of the outer cortex, particularly the proximal convoluted cells, show a very rapid and progressive simple atrophy. In contrast, necrosis of individual cells is followed by mitotic activity in atrophic tubular cells of the inner cortex, medulla, and papilla. Subsequently, polyploidy and hyperplasia occur in the inner cortex. At the same time, hypertrophy of the protein-synthesizing apparatus and an increase in protein, DNA, and RNA, followed by a decrease in the protein content, are seen in the tubular cells of the inner cortex. In the medulla and papilla, necrosis of individual cells proceeds side by side with waves of mitotic activity. These events take place, albeit to a lesser degree, even in cases of very mild renal ischemia. While they may by unrelated to hypertension, these changes are probably involved in the increase in hydrolytic enzyme activity characteristic of the ischemic renal cortex.", "contents": "Metaplastic and mitotic activity of the ischemic (endocrine) kidney in experimental renal hypertension. Partial ligation of the aorta between the renal arteries in the rat induces malignant hypertension, metaplasia of smooth-muscle cells of arterioles and arteries into juxtaglomerular cells, and a complex series of events in tubular cells at all levels of the ischemic kidney. The tubular cells of the outer cortex, particularly the proximal convoluted cells, show a very rapid and progressive simple atrophy. In contrast, necrosis of individual cells is followed by mitotic activity in atrophic tubular cells of the inner cortex, medulla, and papilla. Subsequently, polyploidy and hyperplasia occur in the inner cortex. At the same time, hypertrophy of the protein-synthesizing apparatus and an increase in protein, DNA, and RNA, followed by a decrease in the protein content, are seen in the tubular cells of the inner cortex. In the medulla and papilla, necrosis of individual cells proceeds side by side with waves of mitotic activity. These events take place, albeit to a lesser degree, even in cases of very mild renal ischemia. While they may by unrelated to hypertension, these changes are probably involved in the increase in hydrolytic enzyme activity characteristic of the ischemic renal cortex."}
{"id": "PMID:474710", "title": "Platelet-leukocyte interactions following arterial endothelial injury.", "content": "Desquamation of canine arterial endothelium was induced by stripping the luminal surface of arteries with an inflated embolectomy catheter. The dogs were killed by intravascular perfusion fixation at intervals varying from 2 hours to 12 weeks after the procedure, and the arteries were studied by light microscopy, transmission electron microscopy, and scanning electron microscopy. A heretofore unreported, apparently specific interaction of aggregated platelets and polymorphonuclear leukocytes (PMNs) was observed on the luminal surface of the denuded arteries. This interaction was abolished by the administration of a single dose of aspirin prior to the application of the catheter. These observations suggest that this platelet--PMN interaction is distinctly different from the classic inflammatory reaction, and that it is an integral part of the arterial response to injury that may affect endothelial regeneration.", "contents": "Platelet-leukocyte interactions following arterial endothelial injury. Desquamation of canine arterial endothelium was induced by stripping the luminal surface of arteries with an inflated embolectomy catheter. The dogs were killed by intravascular perfusion fixation at intervals varying from 2 hours to 12 weeks after the procedure, and the arteries were studied by light microscopy, transmission electron microscopy, and scanning electron microscopy. A heretofore unreported, apparently specific interaction of aggregated platelets and polymorphonuclear leukocytes (PMNs) was observed on the luminal surface of the denuded arteries. This interaction was abolished by the administration of a single dose of aspirin prior to the application of the catheter. These observations suggest that this platelet--PMN interaction is distinctly different from the classic inflammatory reaction, and that it is an integral part of the arterial response to injury that may affect endothelial regeneration."}
{"id": "PMID:474711", "title": "Hemochromatosis caused by excessive vitamin iron intake.", "content": "Rare cases of hemochromatosis have been reported in patients who underwent prolonged oral iron therapy for hemolytic anemia or prolonged self-treatment with iron pills. A proportionately large segment of the South African Bantu tribe, who ingest large quantities of an alcoholic beverage brewed in iron pots, are found to have the disease. Reports of health fadists developing hemochromatosis due to excessive dietary iron intake, however, are extremely rare. This report presents clinical considerations and pathologic findings in a compulsive health fadist who consumed large numbers of vitamins containing iron. Clinical findings included the development and progression of cirrhosis of the liver, bronzing of the skin, and diabetes mellitus, all consistent with a diagnosis of hemochromatosis. Light microscopy of liver biopsies taken late in the course of the disease revealed a massive buildup of iron in the hepatocytes, less in the Kupffer cells, and sparse deposition in the epithelial cells of the bile duct. Minimal periportal fibrosis was noted. Electron microscopy showed numerous pleomorphic siderosomes with varying degrees of crystallization and ferritin attached at uniform intervals to the membranes of residual bodies. Abundant free ferritin was observed in most cells. The aggregated and membrane-associated ferritin was verified by non-dispersive x-ray analysis. An additional finding, noted only by electron microscopy, was the presence of many fat-storing cells of Ito, which are thought to be involved in the onset of fibrosis.", "contents": "Hemochromatosis caused by excessive vitamin iron intake. Rare cases of hemochromatosis have been reported in patients who underwent prolonged oral iron therapy for hemolytic anemia or prolonged self-treatment with iron pills. A proportionately large segment of the South African Bantu tribe, who ingest large quantities of an alcoholic beverage brewed in iron pots, are found to have the disease. Reports of health fadists developing hemochromatosis due to excessive dietary iron intake, however, are extremely rare. This report presents clinical considerations and pathologic findings in a compulsive health fadist who consumed large numbers of vitamins containing iron. Clinical findings included the development and progression of cirrhosis of the liver, bronzing of the skin, and diabetes mellitus, all consistent with a diagnosis of hemochromatosis. Light microscopy of liver biopsies taken late in the course of the disease revealed a massive buildup of iron in the hepatocytes, less in the Kupffer cells, and sparse deposition in the epithelial cells of the bile duct. Minimal periportal fibrosis was noted. Electron microscopy showed numerous pleomorphic siderosomes with varying degrees of crystallization and ferritin attached at uniform intervals to the membranes of residual bodies. Abundant free ferritin was observed in most cells. The aggregated and membrane-associated ferritin was verified by non-dispersive x-ray analysis. An additional finding, noted only by electron microscopy, was the presence of many fat-storing cells of Ito, which are thought to be involved in the onset of fibrosis."}
{"id": "PMID:474712", "title": "Studies on the rat liver following iron overload. Electron probe x-ray microanalysis of acid phosphatase and iron.", "content": "Previous electron-microscopic studies on the liver have shown that following excessive administration of iron to experimental animals, small particles believed to represent ferritin and/or hemosiderin (electron-dense iron-containing particles [IPs]) accumulate in membrane-bound bodies--many with a lysosome-like structure--in liver parenchymal and Kupffer cells. Further identification of the IP-containing bodies has been facilitated by the application of histochemical techniques for the demonstration of acid phosphatase. The results have shown that reaction product was deposited over organelles similar in appearance to the IP-containing ones, indicating that they were lysosomes. However, the granular nature of the reaction product makes it difficult or impossible to decide whether IPs are present simultaneously with reaction product in the organelle. In order to clarify this qualitative aspect, x-ray microanalysis has been utilized to identify iron and lead (reaction product) in the various structures thought to represent lysosomes. The results indicate that all IP-containing bodies also show the presence of reaction product, and thus can be regarded as lysosomes. However, in the parenchymal cells there may exist a small population of iron-deficient lysosomes (only lead could be shown). The latter may correspond to \"primary lysosomes.\"", "contents": "Studies on the rat liver following iron overload. Electron probe x-ray microanalysis of acid phosphatase and iron. Previous electron-microscopic studies on the liver have shown that following excessive administration of iron to experimental animals, small particles believed to represent ferritin and/or hemosiderin (electron-dense iron-containing particles [IPs]) accumulate in membrane-bound bodies--many with a lysosome-like structure--in liver parenchymal and Kupffer cells. Further identification of the IP-containing bodies has been facilitated by the application of histochemical techniques for the demonstration of acid phosphatase. The results have shown that reaction product was deposited over organelles similar in appearance to the IP-containing ones, indicating that they were lysosomes. However, the granular nature of the reaction product makes it difficult or impossible to decide whether IPs are present simultaneously with reaction product in the organelle. In order to clarify this qualitative aspect, x-ray microanalysis has been utilized to identify iron and lead (reaction product) in the various structures thought to represent lysosomes. The results indicate that all IP-containing bodies also show the presence of reaction product, and thus can be regarded as lysosomes. However, in the parenchymal cells there may exist a small population of iron-deficient lysosomes (only lead could be shown). The latter may correspond to \"primary lysosomes.\""}
{"id": "PMID:474715", "title": "Motility of rabbit alveolar cells: role of unsaturated fatty acids.", "content": "A mechanism for the specific accumulation of macrophages in alveoli or other biologic cavities following injury is presented. The data herein indicate that unsaturated fatty acids, ie, linoleic and linolenic acids, which accumulate in rat pleura following injection of carrageenan or during incubation of rabbit alveolar macrophages (AMs), strongly activate migration in vitro of AMs but not of human polymorphonuclear leukocytes (PMNLs). Other anionic lipids, ie, phosphatidylglycerol, as well as various nonspecific proteins, such as gelatin, or albumin were also shown to be potent activators of migration of AMs and not of PMNLs. These observations suggest that the elaboration of unsaturated fatty acids, as well as of nonspecific proteins, is responsible for the specific accumulation of macrophages in injured body spaces, such as alveoli or pleura.", "contents": "Motility of rabbit alveolar cells: role of unsaturated fatty acids. A mechanism for the specific accumulation of macrophages in alveoli or other biologic cavities following injury is presented. The data herein indicate that unsaturated fatty acids, ie, linoleic and linolenic acids, which accumulate in rat pleura following injection of carrageenan or during incubation of rabbit alveolar macrophages (AMs), strongly activate migration in vitro of AMs but not of human polymorphonuclear leukocytes (PMNLs). Other anionic lipids, ie, phosphatidylglycerol, as well as various nonspecific proteins, such as gelatin, or albumin were also shown to be potent activators of migration of AMs and not of PMNLs. These observations suggest that the elaboration of unsaturated fatty acids, as well as of nonspecific proteins, is responsible for the specific accumulation of macrophages in injured body spaces, such as alveoli or pleura."}
{"id": "PMID:474716", "title": "Malignant melanoma in the Sinclair miniature swine: an autopsy study of 60 cases.", "content": "Sinclair miniature swine spontaneously develop multiple cutaneous melanomas which have the ability to metastasize and regress. This study, based on 60 necropsies, documents the similarity of the pathology of the cutaneous malignant melanomas and the organ distribution of metastasis to human melanoma. The invasive cutaneous melanomas have an intraepidermal component analogous to human superficial spreading melanoma. The pathology of the spontaneous regression, characterized by a series of cellular events beginning with a mononuclear inflammatory infiltrate and leading to depigmentation and fibrosis, is likewise similar to cutaneous regression in human melanoma. Just as with human melanoma,metastasis was correlated with deeply invasive cutaneous tumors. Because of both the biologic and histologic similarity of this animal model to human melanoma, the Sinclair miniature swine should serve as an important resource in continuing the study of melanoma.", "contents": "Malignant melanoma in the Sinclair miniature swine: an autopsy study of 60 cases. Sinclair miniature swine spontaneously develop multiple cutaneous melanomas which have the ability to metastasize and regress. This study, based on 60 necropsies, documents the similarity of the pathology of the cutaneous malignant melanomas and the organ distribution of metastasis to human melanoma. The invasive cutaneous melanomas have an intraepidermal component analogous to human superficial spreading melanoma. The pathology of the spontaneous regression, characterized by a series of cellular events beginning with a mononuclear inflammatory infiltrate and leading to depigmentation and fibrosis, is likewise similar to cutaneous regression in human melanoma. Just as with human melanoma,metastasis was correlated with deeply invasive cutaneous tumors. Because of both the biologic and histologic similarity of this animal model to human melanoma, the Sinclair miniature swine should serve as an important resource in continuing the study of melanoma."}
{"id": "PMID:474717", "title": "Alteration of the structure and function of guinea pig peritoneal macrophages by a soybean oil emulsion.", "content": "Studies in humans who have received Intralipid (IL) have demonstrated the presence of a fat pigment and fat droplets in reticuloendothelial phagocytic cells. Clinical data and in vitro studies suggest that these cells do not function normally. We have studied the effect of IL on the morphology and function of guinea pig peritoneal macrophages in vitro. Starch-induced macrophages were exposed to IL for up to 48 hours. Ingestion of increasing amounts of IL over the 48-hour period was confirmed by transmission electron microscopy and by oil red O stain. The uptake of the IL was associated with marked morphologic changes characterized by a decreased ability of the cells to spread and by a decrease in the number and degree of complexity of the membrane ruffles. The ingestion of IL also resulted in decreased capacity of the cells to associate with latex beads (5.7 mu in diameter) or Candida albicans and decreased capacity to adhere to and ingest sheep erythrocytes coated with IgG. After ingestion of latex beads 0.46 mu in diameter, which are similar in size to IL particles, macrophages had normal morphology and function, indicating that neither the morphologic nor functional abnormalities were due to a nonspecific effect of ingestion of small particles. Alterations of human reticuloendothelial macrophage function similar to the effects observed here could compromise host defense against infection.", "contents": "Alteration of the structure and function of guinea pig peritoneal macrophages by a soybean oil emulsion. Studies in humans who have received Intralipid (IL) have demonstrated the presence of a fat pigment and fat droplets in reticuloendothelial phagocytic cells. Clinical data and in vitro studies suggest that these cells do not function normally. We have studied the effect of IL on the morphology and function of guinea pig peritoneal macrophages in vitro. Starch-induced macrophages were exposed to IL for up to 48 hours. Ingestion of increasing amounts of IL over the 48-hour period was confirmed by transmission electron microscopy and by oil red O stain. The uptake of the IL was associated with marked morphologic changes characterized by a decreased ability of the cells to spread and by a decrease in the number and degree of complexity of the membrane ruffles. The ingestion of IL also resulted in decreased capacity of the cells to associate with latex beads (5.7 mu in diameter) or Candida albicans and decreased capacity to adhere to and ingest sheep erythrocytes coated with IgG. After ingestion of latex beads 0.46 mu in diameter, which are similar in size to IL particles, macrophages had normal morphology and function, indicating that neither the morphologic nor functional abnormalities were due to a nonspecific effect of ingestion of small particles. Alterations of human reticuloendothelial macrophage function similar to the effects observed here could compromise host defense against infection."}
{"id": "PMID:474718", "title": "The Ch\u00e9diak-Higashi syndrome: ring-shaped lysomomes in circulating monocytes.", "content": "The giant inclusion anomaly of leukocytes from three patients with the Ch\u00e9diak-Higashi syndrome (CHS) has been reexamined by the ultrastructural and cytochemical techniques placing emphasis on the monocyte. Contrary to previous assumptions that all CHS leukocytes shared a common form of giant granule with similar mechanisms of formation, it is shown that CHS monocytes contain ring-shaped organelles as their principle manifestation of the disease. These granules consist of a double membrane surrounding a segment of cytoplasm, which may or may not be sequestered from the remaining cytoplasm and which usually contains one or more normal-sized azurophilic granules. The material lying between the double membranes contains the specific reaction products for peroxidase and acid phosphatase. Therefore, the ring-shaped organelles share identity with the azurophilic granules and probably share a common origin with the primary lysosomes. The double-ring form of giant CHS organelle has also been found infrequently in neutrophils, eosinophils, and lymphocytes.", "contents": "The Ch\u00e9diak-Higashi syndrome: ring-shaped lysomomes in circulating monocytes. The giant inclusion anomaly of leukocytes from three patients with the Ch\u00e9diak-Higashi syndrome (CHS) has been reexamined by the ultrastructural and cytochemical techniques placing emphasis on the monocyte. Contrary to previous assumptions that all CHS leukocytes shared a common form of giant granule with similar mechanisms of formation, it is shown that CHS monocytes contain ring-shaped organelles as their principle manifestation of the disease. These granules consist of a double membrane surrounding a segment of cytoplasm, which may or may not be sequestered from the remaining cytoplasm and which usually contains one or more normal-sized azurophilic granules. The material lying between the double membranes contains the specific reaction products for peroxidase and acid phosphatase. Therefore, the ring-shaped organelles share identity with the azurophilic granules and probably share a common origin with the primary lysosomes. The double-ring form of giant CHS organelle has also been found infrequently in neutrophils, eosinophils, and lymphocytes."}
{"id": "PMID:474719", "title": "A possible role of arachidonic acid in human neutrophil aggregation and degranulation.", "content": "Chemotactic factors stimulate neutrophils to aggregate and, in the presence of cytochalasin B, to degranulate. Recently, the authors found that arachidonic acid also stimulates human neutrophils to aggregate but does not stimulate cytochalasin-B-treated or untreated cells to degranulate. In this report the authors examined the effect of three blockers of arachidonic acid metabolism on these cellular responses. It was found that the arachidonic acid analog 5,8,11,14-eicosatetraynoic acid and indomethacin, but not aspirin, inhibited no only the arachidonic-acid-induced aggregation response but also the degranulation responses evoked  by C5a or a synthetic oligopeptide chemotactic factor. These results suggest that arachidonic acid may be a precursor of bioactive metabolites that stimulate the aggregation and foster the degranulation responses of neutrophils. Thus, these metabolites may be mediators of neutrophil function. Agents that block their formation may thereby inhibit aggregation and degranulation.", "contents": "A possible role of arachidonic acid in human neutrophil aggregation and degranulation. Chemotactic factors stimulate neutrophils to aggregate and, in the presence of cytochalasin B, to degranulate. Recently, the authors found that arachidonic acid also stimulates human neutrophils to aggregate but does not stimulate cytochalasin-B-treated or untreated cells to degranulate. In this report the authors examined the effect of three blockers of arachidonic acid metabolism on these cellular responses. It was found that the arachidonic acid analog 5,8,11,14-eicosatetraynoic acid and indomethacin, but not aspirin, inhibited no only the arachidonic-acid-induced aggregation response but also the degranulation responses evoked  by C5a or a synthetic oligopeptide chemotactic factor. These results suggest that arachidonic acid may be a precursor of bioactive metabolites that stimulate the aggregation and foster the degranulation responses of neutrophils. Thus, these metabolites may be mediators of neutrophil function. Agents that block their formation may thereby inhibit aggregation and degranulation."}
{"id": "PMID:474720", "title": "Morphologic studies in the skeletal dysplasias.", "content": "Considerable progress has been made in the delineation of the genetic skeletal dysplasias, a heterogeneous group of disorders, that consist of over 80 distinct conditions. Morphologic studies have added a further dimension to the delineation of these conditions, their diagnosis, and the investigation of their pathogenetic mechanisms. In certain diseases, the morphologic alterations are characteristic and pathognomonic. In others only nonspecific alterations are observed, whereas in still other disorders growth-plate structure is essentially normal. Histologic, histochemical, and electronmicroscopic studies of growth-plate cartilage have provided new insights into the complexity of morphogenetic events in normal growth through the demonstration of morphologic defects in the genetic disorders of skeletal growth. As yet, very little is known of the biochemical abnormalities underlying the morphologic abnormalities. However, the great variety of morphologic findings points to a number of different pathogenetic defects in the synthesis, release, and assembly of connective tissue macromolecules and in the cells involved in growth-plate metabolism.", "contents": "Morphologic studies in the skeletal dysplasias. Considerable progress has been made in the delineation of the genetic skeletal dysplasias, a heterogeneous group of disorders, that consist of over 80 distinct conditions. Morphologic studies have added a further dimension to the delineation of these conditions, their diagnosis, and the investigation of their pathogenetic mechanisms. In certain diseases, the morphologic alterations are characteristic and pathognomonic. In others only nonspecific alterations are observed, whereas in still other disorders growth-plate structure is essentially normal. Histologic, histochemical, and electronmicroscopic studies of growth-plate cartilage have provided new insights into the complexity of morphogenetic events in normal growth through the demonstration of morphologic defects in the genetic disorders of skeletal growth. As yet, very little is known of the biochemical abnormalities underlying the morphologic abnormalities. However, the great variety of morphologic findings points to a number of different pathogenetic defects in the synthesis, release, and assembly of connective tissue macromolecules and in the cells involved in growth-plate metabolism."}
{"id": "PMID:474725", "title": "Mental health in a fragmented society: the shattered crystal ball.", "content": "Effects of current social and political forces on mental health are considered, with particular attention to the way in which the unrelenting acceleration of change undermines the individual's sense of temporal continuity. The contributions and limitations of the mental health professions are examined, and it is suggested that the major challenge of psychotherapy in our time may be to aid individuals in coping with a world in flux.", "contents": "Mental health in a fragmented society: the shattered crystal ball. Effects of current social and political forces on mental health are considered, with particular attention to the way in which the unrelenting acceleration of change undermines the individual's sense of temporal continuity. The contributions and limitations of the mental health professions are examined, and it is suggested that the major challenge of psychotherapy in our time may be to aid individuals in coping with a world in flux."}
{"id": "PMID:474726", "title": "The use of two houses in play therapy.", "content": "The use of two miniature house structures equipped with dolls is proposed in play therapy with children who have been traumatized by physical loss, emotional withdrawal, or rejection of family members. The case of a child who worked through unresolved conflicts resulting from separation and placement is described. Feelings of rejection, anger, fear, and guilt were reexperienced in the \"old\" house, while the second house provided a more positive alternative. Implications of the approach for children who have experienced other types of family crisis are discussed.", "contents": "The use of two houses in play therapy. The use of two miniature house structures equipped with dolls is proposed in play therapy with children who have been traumatized by physical loss, emotional withdrawal, or rejection of family members. The case of a child who worked through unresolved conflicts resulting from separation and placement is described. Feelings of rejection, anger, fear, and guilt were reexperienced in the \"old\" house, while the second house provided a more positive alternative. Implications of the approach for children who have experienced other types of family crisis are discussed."}
{"id": "PMID:474727", "title": "The character-disordered family: a community treatment model for family sexual abuse.", "content": "A collaborative approach to treating sexually abusive families is described, in which such families are viewed as analogous to \"character-disordered\" individuals. This model, unlike traditional voluntary treatment models, assumes that effective intervention requires authoritative control and careful corrdination of all professional activity. Phases of treatment are outlined and a case history is presented.", "contents": "The character-disordered family: a community treatment model for family sexual abuse. A collaborative approach to treating sexually abusive families is described, in which such families are viewed as analogous to \"character-disordered\" individuals. This model, unlike traditional voluntary treatment models, assumes that effective intervention requires authoritative control and careful corrdination of all professional activity. Phases of treatment are outlined and a case history is presented."}
{"id": "PMID:474728", "title": "A model of the empathic process for teachers of emotionally disturbed children.", "content": "A model for the demonstration of empathy by teachers, based on interviews with emotionally disturbed youngsters, is described. It is suggested that verbalization of understanding is not the complete process and that at least four interactive modalities are used by teachers to express empathy with a disturbed child.", "contents": "A model of the empathic process for teachers of emotionally disturbed children. A model for the demonstration of empathy by teachers, based on interviews with emotionally disturbed youngsters, is described. It is suggested that verbalization of understanding is not the complete process and that at least four interactive modalities are used by teachers to express empathy with a disturbed child."}
{"id": "PMID:474729", "title": "Infant behavior and maternal adaptations in the first six months of life.", "content": "This study explored the relationship among infant temperament, maternal concern, and adjustment during early infancy, and the stability of these variables over time. Infants identified as difficult were found to change considerably across three time points (between five weeks and six months), suggesting the need for caution in prognostication with respect to temperament difficulties in infancy. Further implications for the care of young infants and their families are discussed.", "contents": "Infant behavior and maternal adaptations in the first six months of life. This study explored the relationship among infant temperament, maternal concern, and adjustment during early infancy, and the stability of these variables over time. Infants identified as difficult were found to change considerably across three time points (between five weeks and six months), suggesting the need for caution in prognostication with respect to temperament difficulties in infancy. Further implications for the care of young infants and their families are discussed."}
{"id": "PMID:474730", "title": "Early death of a parent as an etiological factor in schizophrenia.", "content": "Results of three separate studies comparing adult schizophrenic and other psychiatric patients suggest that premature death of a parent may be a contributing factor in the etiology of schizophrenia. This finding was not attributable to a genetically-linked tendency toward suicide in parents. Parental death was found to occur earlier among schizophrenics than other patients and, in two studies, to be most frequent among schizophrenics with predominantly paranoid symptoms.", "contents": "Early death of a parent as an etiological factor in schizophrenia. Results of three separate studies comparing adult schizophrenic and other psychiatric patients suggest that premature death of a parent may be a contributing factor in the etiology of schizophrenia. This finding was not attributable to a genetically-linked tendency toward suicide in parents. Parental death was found to occur earlier among schizophrenics than other patients and, in two studies, to be most frequent among schizophrenics with predominantly paranoid symptoms."}
{"id": "PMID:474731", "title": "The role of cognitive level and sibling illness in children's conceptualizations of illness.", "content": "Levels of illness conceptualization were studied among children with diabetic siblings. Results revealed a significant association between pretested Piagetian level of cognitive development and illness conceptualization. Children with ill siblings, especially those at the formal operational level,  demonstrated lower conceptualization levels than did children with healthy siblings.", "contents": "The role of cognitive level and sibling illness in children's conceptualizations of illness. Levels of illness conceptualization were studied among children with diabetic siblings. Results revealed a significant association between pretested Piagetian level of cognitive development and illness conceptualization. Children with ill siblings, especially those at the formal operational level,  demonstrated lower conceptualization levels than did children with healthy siblings."}
{"id": "PMID:474732", "title": "Stimulant drugs and activity level in hyperactive children.", "content": "This study of 14 hyperactive boys reports that activity and attention span appear to be affected by methylphenidate even in highly stimulating, informal settings. Comparison of the hyperactive boys with a group of 14 normal controls suggests that this drug induced reduction of activity and inattentiveness is not a \"normalizing\" effect. Implications of the findings are discussed.", "contents": "Stimulant drugs and activity level in hyperactive children. This study of 14 hyperactive boys reports that activity and attention span appear to be affected by methylphenidate even in highly stimulating, informal settings. Comparison of the hyperactive boys with a group of 14 normal controls suggests that this drug induced reduction of activity and inattentiveness is not a \"normalizing\" effect. Implications of the findings are discussed."}
{"id": "PMID:474740", "title": "Differences in ammonia and adenylate metabolism in contracting fast and slow muscle.", "content": "The time course of AMP deamination and IMP reamination was studied during in situ stimulation and subsequent recovery in fast-twitch (gastrocnemius) and slow-twitch (soleus) muscles of pentobarbital-anesthetized rats. Muscles were stimulated tetanically at rates that initially produced comparable decreases (40%) in tension development. In fast muscle, progressive decreases in total adenine nucleotide (TAN) contents of up to 50% were balanced by equivalent increases in IMP contents. Ammonia concentration initially increased in a 1:1 stoichiometry with changes in IMP and TAN. During recovery following stimulation, IMP removal matched, but NH3 removal exceeded the rate of TAN resynthesis. In contrast, TAN content in slow muscle was only slightly decreased (10%) during stimulation and there were no increases in IMP or NH3. Stimulation of the soleus following ligation of the blood supply did not increase TAN depletion. In both fast and slow muscle, changes in glutamate, aspartate, and alanine could be accounted for by transamination. These results illustrate a fundamental difference in adenylate metabolism during intense muscle stimulation between fast- and slow-twitch mammalian muscle.", "contents": "Differences in ammonia and adenylate metabolism in contracting fast and slow muscle. The time course of AMP deamination and IMP reamination was studied during in situ stimulation and subsequent recovery in fast-twitch (gastrocnemius) and slow-twitch (soleus) muscles of pentobarbital-anesthetized rats. Muscles were stimulated tetanically at rates that initially produced comparable decreases (40%) in tension development. In fast muscle, progressive decreases in total adenine nucleotide (TAN) contents of up to 50% were balanced by equivalent increases in IMP contents. Ammonia concentration initially increased in a 1:1 stoichiometry with changes in IMP and TAN. During recovery following stimulation, IMP removal matched, but NH3 removal exceeded the rate of TAN resynthesis. In contrast, TAN content in slow muscle was only slightly decreased (10%) during stimulation and there were no increases in IMP or NH3. Stimulation of the soleus following ligation of the blood supply did not increase TAN depletion. In both fast and slow muscle, changes in glutamate, aspartate, and alanine could be accounted for by transamination. These results illustrate a fundamental difference in adenylate metabolism during intense muscle stimulation between fast- and slow-twitch mammalian muscle."}
{"id": "PMID:474741", "title": "Effect of stretch on conduction velocity and cable properties of cardiac Purkinje fibers.", "content": "Cardiac Purkinje fibers were studied before and after stretch to 30% and 50% in excess of their slack length, and membrane properties and conduction velocity were measured in relation to the stretch. Conduction velocity increased by 26% with 50% stretch. The resting potentials averaged -77 mV and did not change with stretch. In addition, the action potential height, maximal upstroke velocity, duration, and time constant of the foot did not change. These results suggested that the increase in conduction velocity was not due to a change in membrane excitability. Two geometric models of stretch, called unfolding and uncoiling, were considered. Cable analyses were performed to distinguish between these models. The effects were mixed, with about 2/3 of the length change resulting from unfolding and 1/3 from uncoiling. These results support the concept of redundancy of the surface membrane by folding. The changes are likely to be of importance in activation of the heart by the His-Purkinje system, especially in cardiac dilatation due to disease.", "contents": "Effect of stretch on conduction velocity and cable properties of cardiac Purkinje fibers. Cardiac Purkinje fibers were studied before and after stretch to 30% and 50% in excess of their slack length, and membrane properties and conduction velocity were measured in relation to the stretch. Conduction velocity increased by 26% with 50% stretch. The resting potentials averaged -77 mV and did not change with stretch. In addition, the action potential height, maximal upstroke velocity, duration, and time constant of the foot did not change. These results suggested that the increase in conduction velocity was not due to a change in membrane excitability. Two geometric models of stretch, called unfolding and uncoiling, were considered. Cable analyses were performed to distinguish between these models. The effects were mixed, with about 2/3 of the length change resulting from unfolding and 1/3 from uncoiling. These results support the concept of redundancy of the surface membrane by folding. The changes are likely to be of importance in activation of the heart by the His-Purkinje system, especially in cardiac dilatation due to disease."}
{"id": "PMID:474742", "title": "Suppression of myotonia in dystrophic chicken muscle by phenytoin.", "content": "We describe myotonic electromyographic activity in muscles of genetically dystrophic chickens and show that this activity is antagonized in vivo by doses of phenytoin (DPH) that improve righting ability. To test the possibility that the in vivo effects of DPH can be accounted for by a direct action on skeletal muscle we studied posterior latissimus dorsi fibers in vitro at 23 degrees C with intracellular microelectrodes. Compared to normal fibers, fibers from untreated dystrophic chicks had larger diameters, increased membrane capacitance, longer latencies at rheobase, and a greater tendency to fire repetitively in response to direct stimulation. DPH (10 or 50 micrograms/ml in the bath solution) decreased latencies at rheobase and repetitive firing in fibers from untreated chicks. In DPH-free solution fibers from dystrophic chicks treated chronically with DPH were still abnormal with respect to latencies at rheobase and ease of repetitive firing. The data support the hypothesis that abnormalities of membrane electrical properties are major features of dystrophic chicken muscles and furthermore, show that DPH suppresses, but does not abolish, these abnormalities.", "contents": "Suppression of myotonia in dystrophic chicken muscle by phenytoin. We describe myotonic electromyographic activity in muscles of genetically dystrophic chickens and show that this activity is antagonized in vivo by doses of phenytoin (DPH) that improve righting ability. To test the possibility that the in vivo effects of DPH can be accounted for by a direct action on skeletal muscle we studied posterior latissimus dorsi fibers in vitro at 23 degrees C with intracellular microelectrodes. Compared to normal fibers, fibers from untreated dystrophic chicks had larger diameters, increased membrane capacitance, longer latencies at rheobase, and a greater tendency to fire repetitively in response to direct stimulation. DPH (10 or 50 micrograms/ml in the bath solution) decreased latencies at rheobase and repetitive firing in fibers from untreated chicks. In DPH-free solution fibers from dystrophic chicks treated chronically with DPH were still abnormal with respect to latencies at rheobase and ease of repetitive firing. The data support the hypothesis that abnormalities of membrane electrical properties are major features of dystrophic chicken muscles and furthermore, show that DPH suppresses, but does not abolish, these abnormalities."}
{"id": "PMID:474743", "title": "Calcium transport and contractile activity in dissociated mammalian heart cells.", "content": "A homogeneous population of dissociated myocytes can be obtained by enzymic perfusion of adult rabbit hearts. The external membrane of the dissociated cells is freely permeable to electrolytes and is electrochemically shunted. Nevertheless, in the presence of 0.2--0.6 muM Ca2+, the myocytes undergo phasic contractions. Within the range of Ca2+ concentrations eliciting phasic contractions, the dissociated myocytes exhibit Ca2+ uptake activity that is sustained by endogenous ATP in the presence of oxygen and metabolites, or by hexogenous ATP in the presence of oligomycin and antimycin. The uptake is greatly enhanced by oxalate, an effect that is attributed to the activity of sarcoplasmic reticulum. Addition of the calcium ionophore A23187 prevents the occurrence of both Ca2+ accumulation and phasic contractions. In the presence of the ionophore the myocytes remain relaxed at Ca2+ concentrations (less than 1.0 mM) normally sustaining phasic contractile activity, and undergo irreversible contracture at Ca2+ concentrations (greater than 1.0 muM) sufficient to produce direct activation of the myofibrils. It is concluded that Ca2+ transport by sarcoplasmic reticulum is directly related to the occurrence of phasic contractions in dissociated myocytes prepared from rabbit heart. On the other hand, metabolic activity is required for ATP regeneration.", "contents": "Calcium transport and contractile activity in dissociated mammalian heart cells. A homogeneous population of dissociated myocytes can be obtained by enzymic perfusion of adult rabbit hearts. The external membrane of the dissociated cells is freely permeable to electrolytes and is electrochemically shunted. Nevertheless, in the presence of 0.2--0.6 muM Ca2+, the myocytes undergo phasic contractions. Within the range of Ca2+ concentrations eliciting phasic contractions, the dissociated myocytes exhibit Ca2+ uptake activity that is sustained by endogenous ATP in the presence of oxygen and metabolites, or by hexogenous ATP in the presence of oligomycin and antimycin. The uptake is greatly enhanced by oxalate, an effect that is attributed to the activity of sarcoplasmic reticulum. Addition of the calcium ionophore A23187 prevents the occurrence of both Ca2+ accumulation and phasic contractions. In the presence of the ionophore the myocytes remain relaxed at Ca2+ concentrations (less than 1.0 mM) normally sustaining phasic contractile activity, and undergo irreversible contracture at Ca2+ concentrations (greater than 1.0 muM) sufficient to produce direct activation of the myofibrils. It is concluded that Ca2+ transport by sarcoplasmic reticulum is directly related to the occurrence of phasic contractions in dissociated myocytes prepared from rabbit heart. On the other hand, metabolic activity is required for ATP regeneration."}
{"id": "PMID:474744", "title": "Membrane permeability during low potassium depolarization in sheep cardiac Purkinje fibers.", "content": "Exposure of sheep Purkinje fibers to low [K]o leads to marked depolarization to a stable potential of about -40 mV. This level is equivalent to the plateau of the Purkinje fiber action potential. The low [K]o depolarization could be prevented by removal of [Na]o and was modified by tetrodotoxin. The membrane potential in the depolarized state was unresponsive to changes in [Cl]o or [Ca]o and it was poorly responsive to changes in [K]o between 0 and 2 mM. Repolarization was induced by decrease in [Na]o with a slope response of 30 mV/10-fold change in [Na]o. Average internal K activity (aK) in the resting state with a [K]o of 5 mM was 121.4 mM for a membrane potential of -80 mV. During low K depolarization aK was 119.7 mM with a membrane potential of -34 mV. The depolarization was therefore due to a change in membrane permeability, with little change in aK. Upon restoration of [K]o the fiber repolarized to values transiently more negative than the prior resting potential. These transient potentials were more negative than the K equilibrium potential (VK), if it is calculated assuming a uniform [K]o. The hyperpolarization was reduced by ouabain [10(-6)] or by low [Ca]o.", "contents": "Membrane permeability during low potassium depolarization in sheep cardiac Purkinje fibers. Exposure of sheep Purkinje fibers to low [K]o leads to marked depolarization to a stable potential of about -40 mV. This level is equivalent to the plateau of the Purkinje fiber action potential. The low [K]o depolarization could be prevented by removal of [Na]o and was modified by tetrodotoxin. The membrane potential in the depolarized state was unresponsive to changes in [Cl]o or [Ca]o and it was poorly responsive to changes in [K]o between 0 and 2 mM. Repolarization was induced by decrease in [Na]o with a slope response of 30 mV/10-fold change in [Na]o. Average internal K activity (aK) in the resting state with a [K]o of 5 mM was 121.4 mM for a membrane potential of -80 mV. During low K depolarization aK was 119.7 mM with a membrane potential of -34 mV. The depolarization was therefore due to a change in membrane permeability, with little change in aK. Upon restoration of [K]o the fiber repolarized to values transiently more negative than the prior resting potential. These transient potentials were more negative than the K equilibrium potential (VK), if it is calculated assuming a uniform [K]o. The hyperpolarization was reduced by ouabain [10(-6)] or by low [Ca]o."}
{"id": "PMID:474745", "title": "Reversible suppression of skeletal myotube formation in vitro obtained by varying [CO2].", "content": "In primary cultures of chicken skeletal muscle, decreasing the [CO2] of the gaseous phase below 10(-3)% resulted in inhibition of cell proliferation and cytolysis. With 10(-3)% CO2-air, cell proliferation was slightly retarded and myotube formation was inhibited approximately 90% compared to cultures receiving 5% CO2-air. Changes in pH were not effective. Culture in low [CO2] resulted in the accumulation of lipoidal inclusions and unique cytoplasmic structures. Increasing time in culture with low [CO2] resulted in an increase in the length of G1 of the cell cycle. The inhibition was reversed by the addition of 5% CO2-air at any time in culture up to 2 wk with a minimum time of 3--6 h required. Lipoidal inclusions decreased in number and the unique cytoplasmic structures were absent. During the first 3 days in culture, myoblasts showing dependence on [CO2] for myotube formation increased in number, and the effect of elevated [CO2] on these cells was long lasting. The data suggest that some aspect of myoblast differentiation relating to cell recognition and fusion is affected by decreased [CO2].", "contents": "Reversible suppression of skeletal myotube formation in vitro obtained by varying [CO2]. In primary cultures of chicken skeletal muscle, decreasing the [CO2] of the gaseous phase below 10(-3)% resulted in inhibition of cell proliferation and cytolysis. With 10(-3)% CO2-air, cell proliferation was slightly retarded and myotube formation was inhibited approximately 90% compared to cultures receiving 5% CO2-air. Changes in pH were not effective. Culture in low [CO2] resulted in the accumulation of lipoidal inclusions and unique cytoplasmic structures. Increasing time in culture with low [CO2] resulted in an increase in the length of G1 of the cell cycle. The inhibition was reversed by the addition of 5% CO2-air at any time in culture up to 2 wk with a minimum time of 3--6 h required. Lipoidal inclusions decreased in number and the unique cytoplasmic structures were absent. During the first 3 days in culture, myoblasts showing dependence on [CO2] for myotube formation increased in number, and the effect of elevated [CO2] on these cells was long lasting. The data suggest that some aspect of myoblast differentiation relating to cell recognition and fusion is affected by decreased [CO2]."}
{"id": "PMID:474748", "title": "Changes in pituitary and thyroid function with increasing age in young male rats.", "content": "As the age of young adult male rats increased from 30 to 150 days, the serum thyroxine (T4) decreased by 50% and the serum thyroid-stimulating hormone (TSH) increased by 250%. There was no change in the serum triiodothyronine (T3). The increment in serum TSH after injection of thyrotropin-releasing hormone (TRH) was not significantly different at any of the ages studied, but the old animals had significantly lower increments in serum T4 and T3 after subcutaneous administration of bovine TSH. Despite a higher basal serum TSH, the older rats had a lesser increase in serum TSH after thyroidectomy or propylthiouracil. Thus, 1) there is a progressive decline in intrinsic thyroid function between 30 and 150 days of age in male rats, and 2) pituitary TSH response to fall in serum concentration of thyroid hormones is also decreased with age.", "contents": "Changes in pituitary and thyroid function with increasing age in young male rats. As the age of young adult male rats increased from 30 to 150 days, the serum thyroxine (T4) decreased by 50% and the serum thyroid-stimulating hormone (TSH) increased by 250%. There was no change in the serum triiodothyronine (T3). The increment in serum TSH after injection of thyrotropin-releasing hormone (TRH) was not significantly different at any of the ages studied, but the old animals had significantly lower increments in serum T4 and T3 after subcutaneous administration of bovine TSH. Despite a higher basal serum TSH, the older rats had a lesser increase in serum TSH after thyroidectomy or propylthiouracil. Thus, 1) there is a progressive decline in intrinsic thyroid function between 30 and 150 days of age in male rats, and 2) pituitary TSH response to fall in serum concentration of thyroid hormones is also decreased with age."}
{"id": "PMID:474749", "title": "Effect of winter sleep on pituitary-thyroid axis in American black bear.", "content": "During winter sleep the black bear has decreased levels of serum total and free thyroxine (T4) and triiodothyronine (T3) and a prolonged, delayed response of serum thyrotropin (TSH) (bioassay) to thyrotropin-releasing hormone (TRH). Four weeks after the end of winter sleep, levels of serum thyroid hormones increase, and TSH response to TRH is short and brisk. Serum T4 and T3 rise after TRH administration both during and after winter sleep; however, the maximum increment in serum T3 is greater during winter sleep when the TSH rise is also prolonged and exaggerated. These observations suggest that transient hypothyroidism of possible hypothalamic origin occurs in bears during winter sleep.", "contents": "Effect of winter sleep on pituitary-thyroid axis in American black bear. During winter sleep the black bear has decreased levels of serum total and free thyroxine (T4) and triiodothyronine (T3) and a prolonged, delayed response of serum thyrotropin (TSH) (bioassay) to thyrotropin-releasing hormone (TRH). Four weeks after the end of winter sleep, levels of serum thyroid hormones increase, and TSH response to TRH is short and brisk. Serum T4 and T3 rise after TRH administration both during and after winter sleep; however, the maximum increment in serum T3 is greater during winter sleep when the TSH rise is also prolonged and exaggerated. These observations suggest that transient hypothyroidism of possible hypothalamic origin occurs in bears during winter sleep."}
{"id": "PMID:474751", "title": "Steroid secretion by in vitro perfused testes: testosterone biosynthetic pathways.", "content": "Alternative metabolic pathways for the biosynthesis of testosterone from pregnenolone exist in mammalian testes. The following experiments were designed to identify the preferred testosterone biosynthetic pathway in rat and rabbit testes. The experimental protocol included the infusion of steroidogenic reaction inhibitors and testosterone biosynthetic intermediates into testes perfused in vitro. Under these conditions, the testicular steroid secretions were a measure of specific reaction activities. Infusion of medrogestone (6,17-dimethyl-4,6-pregnadiene-3,20-dione, Ayerst), an inhibitor of delta5-4isomerization, permitted the reactions converting pregnenolone to androstenediol to be studied separately from those converting progesterone to testosterone. For example, the activity of the pregnenolone vector 17alpha-hydroxypregnenolone reaction was measured as the total of the 17alpha-hydroxypregnenolone, dehydroepiandrosterone, and androstenediol secreted by medrogestone-inhibited testes. The reactions convering delta5-3beta-hydroxysteroids to delta4-3-ketosteroids were studied in testes infused with SU-10603 (7-chloro-3,4-dihydro-2-[3-pyridyl]-1(2H)-naphthalenone. Ciba-Geigy), an inhibitor of 17alpha-hydroxylation and C-17, C-20 cleavage reactions. The results indicated that preferred testosterone biosynthetic pathways are present and different in rat and rabbit testes.", "contents": "Steroid secretion by in vitro perfused testes: testosterone biosynthetic pathways. Alternative metabolic pathways for the biosynthesis of testosterone from pregnenolone exist in mammalian testes. The following experiments were designed to identify the preferred testosterone biosynthetic pathway in rat and rabbit testes. The experimental protocol included the infusion of steroidogenic reaction inhibitors and testosterone biosynthetic intermediates into testes perfused in vitro. Under these conditions, the testicular steroid secretions were a measure of specific reaction activities. Infusion of medrogestone (6,17-dimethyl-4,6-pregnadiene-3,20-dione, Ayerst), an inhibitor of delta5-4isomerization, permitted the reactions converting pregnenolone to androstenediol to be studied separately from those converting progesterone to testosterone. For example, the activity of the pregnenolone vector 17alpha-hydroxypregnenolone reaction was measured as the total of the 17alpha-hydroxypregnenolone, dehydroepiandrosterone, and androstenediol secreted by medrogestone-inhibited testes. The reactions convering delta5-3beta-hydroxysteroids to delta4-3-ketosteroids were studied in testes infused with SU-10603 (7-chloro-3,4-dihydro-2-[3-pyridyl]-1(2H)-naphthalenone. Ciba-Geigy), an inhibitor of 17alpha-hydroxylation and C-17, C-20 cleavage reactions. The results indicated that preferred testosterone biosynthetic pathways are present and different in rat and rabbit testes."}
{"id": "PMID:474753", "title": "Maternal starvation and progesterone secretion, litter size, and growth in the pig.", "content": "The relationships of maternal nutrition and progesterone secretion to prenatal fetal loss and neonatal survival and growth were investigated in domesticated pigs that normally experience 40% fetal loss. Yorkshire pigs were subjected to prolonged starvation (40 days; 0 kcal/day; water only) in either the middle third or last third of pregnancy and then gradually realimented to a full diet and allowed to advance to parturition; controls received a full diet (7,028 kcal/day) throughout gestation. Pregnancies were maintained in 74% of the pigs starved during either middle or late gestation compared to 100% in controls. In dams starved from days 30--70, progesterone levels remained similar to controls, but were maintained at lower levels during realimentation between days 72 and 99. Progesterone levels in dams starved days 50--90 and controls were similar from days 50 to 130. At parturition, litter size in starved dams (9.4) was similar to full-diet controls (8.0) although birth weight and neonatal growth of piglets were reduced markedly from dams starved in middle or late pregnancy. These results indicate that severe maternal nutrient deprivation during the middle or last third of gestation has little effect on ovarian progesterone secretion and is not a major limitation to fetal survival in this litter-bearing species. Maternal nutrient deprivation imposes a significant detrimental effect, however, on birth weight and neonatal growth to 80 days of age.", "contents": "Maternal starvation and progesterone secretion, litter size, and growth in the pig. The relationships of maternal nutrition and progesterone secretion to prenatal fetal loss and neonatal survival and growth were investigated in domesticated pigs that normally experience 40% fetal loss. Yorkshire pigs were subjected to prolonged starvation (40 days; 0 kcal/day; water only) in either the middle third or last third of pregnancy and then gradually realimented to a full diet and allowed to advance to parturition; controls received a full diet (7,028 kcal/day) throughout gestation. Pregnancies were maintained in 74% of the pigs starved during either middle or late gestation compared to 100% in controls. In dams starved from days 30--70, progesterone levels remained similar to controls, but were maintained at lower levels during realimentation between days 72 and 99. Progesterone levels in dams starved days 50--90 and controls were similar from days 50 to 130. At parturition, litter size in starved dams (9.4) was similar to full-diet controls (8.0) although birth weight and neonatal growth of piglets were reduced markedly from dams starved in middle or late pregnancy. These results indicate that severe maternal nutrient deprivation during the middle or last third of gestation has little effect on ovarian progesterone secretion and is not a major limitation to fetal survival in this litter-bearing species. Maternal nutrient deprivation imposes a significant detrimental effect, however, on birth weight and neonatal growth to 80 days of age."}
{"id": "PMID:474754", "title": "Gastric pacemaker rhythm in conscious dog.", "content": "The pacemaker rhythm in the stomach of six healthy conscious dogs was studied by means of the measurement of sequential electrical control activity (ECA) intervals. Only the rhythm originating in the normal pacemake area was studied. An electrical response activity (ERA) score was used to assess contractile activity. Whereas substantial shortening of nonectopic ECA intervals did not occur, contraction-related interval lengthening was a characteristic phenomenon. The temporal relations between ERA scores and interval durations were found to be dependent on the gastric level at which the signals were derived. The activity front of the interdigestive myoelectric complex (IDMEC) appeared to be correlated with the periodic occurrence of considerably prolonged intervals; this sign could be used for recognition of the IDMEC. The motor quiescence phase of the IDMEC was correlated with small interval-to-interval variation, as was the early postprandial phase.", "contents": "Gastric pacemaker rhythm in conscious dog. The pacemaker rhythm in the stomach of six healthy conscious dogs was studied by means of the measurement of sequential electrical control activity (ECA) intervals. Only the rhythm originating in the normal pacemake area was studied. An electrical response activity (ERA) score was used to assess contractile activity. Whereas substantial shortening of nonectopic ECA intervals did not occur, contraction-related interval lengthening was a characteristic phenomenon. The temporal relations between ERA scores and interval durations were found to be dependent on the gastric level at which the signals were derived. The activity front of the interdigestive myoelectric complex (IDMEC) appeared to be correlated with the periodic occurrence of considerably prolonged intervals; this sign could be used for recognition of the IDMEC. The motor quiescence phase of the IDMEC was correlated with small interval-to-interval variation, as was the early postprandial phase."}
{"id": "PMID:474755", "title": "Lymph capillary pressure of rat intestinal villi during fluid absorption.", "content": "A newly developed intestinal preparation is described for determining lymph capillary pressure (PL) in the villi in vivo and in vitro. Determination of PL provided an estimate of tissue fluid pressure in the villi. PL was related to the fluid absorption rate and increased by lymphatic obstruction. During fluid absorption from isotonic mucosal fluid, PL was 1.4 +/- 0.5 or 1.1 +/- 0.4 cmH2O determined in vivo or in vitro, respectively. Both pressures were essentially in the same range as that (0.7 +/- 0.3--1.3 +/- 0.5 cmH2O) in which the mucosal fluid was isotonic Na2SO4 solution or Na-free solutions from which little fluid absorption occurred. This range of pressures may be taken as the normal tissue fluid pressure in the villi. At a high rate of fluid absorption from hypotonic mucosal fluid, PL increased to 5.2 +/- 1.4 cmH2O and tissue fluid pressure was also similarly increased. It is concluded that the fluid absorptive process by the epithelium could not develop an appreciable hydrostatic pressure in the villus tissue space or in the lymphatics.", "contents": "Lymph capillary pressure of rat intestinal villi during fluid absorption. A newly developed intestinal preparation is described for determining lymph capillary pressure (PL) in the villi in vivo and in vitro. Determination of PL provided an estimate of tissue fluid pressure in the villi. PL was related to the fluid absorption rate and increased by lymphatic obstruction. During fluid absorption from isotonic mucosal fluid, PL was 1.4 +/- 0.5 or 1.1 +/- 0.4 cmH2O determined in vivo or in vitro, respectively. Both pressures were essentially in the same range as that (0.7 +/- 0.3--1.3 +/- 0.5 cmH2O) in which the mucosal fluid was isotonic Na2SO4 solution or Na-free solutions from which little fluid absorption occurred. This range of pressures may be taken as the normal tissue fluid pressure in the villi. At a high rate of fluid absorption from hypotonic mucosal fluid, PL increased to 5.2 +/- 1.4 cmH2O and tissue fluid pressure was also similarly increased. It is concluded that the fluid absorptive process by the epithelium could not develop an appreciable hydrostatic pressure in the villus tissue space or in the lymphatics."}
{"id": "PMID:474756", "title": "Optical techniques for the evaluation of epithelial transport processes.", "content": "Light microscopic observation of living epithelial tissues is a powerful analytical tool in the investigation of solute and water transport. New techniques in quantitative microscopy permit the determination of epithelial cell size and shape as well as accurate measurements of lateral intercellular space dimensions. Traditional techniques for the study of epithelial cell function may be used in conjunction with continuous quantitation of tissue morphology. Fluorescent probes of membrane, cell, and interspace function may be monitored at low light levels to obtain information about the dynamic behavior of the preparation. Application of some of these methods to the Necturus gallbladder epithelium has enabled determination of lateral cell membrane compliance, the hydrostatic pressure equivalent to active transport, the intracellular NaCl transport pool, the rate of active NaCl transport, and the flux of NaCl into the cell across the apical membrane. Possible future applications of optical techniques include measurement of water permeability, solute reflection coefficients, fluid flow paterns, and interspace osmolality.", "contents": "Optical techniques for the evaluation of epithelial transport processes. Light microscopic observation of living epithelial tissues is a powerful analytical tool in the investigation of solute and water transport. New techniques in quantitative microscopy permit the determination of epithelial cell size and shape as well as accurate measurements of lateral intercellular space dimensions. Traditional techniques for the study of epithelial cell function may be used in conjunction with continuous quantitation of tissue morphology. Fluorescent probes of membrane, cell, and interspace function may be monitored at low light levels to obtain information about the dynamic behavior of the preparation. Application of some of these methods to the Necturus gallbladder epithelium has enabled determination of lateral cell membrane compliance, the hydrostatic pressure equivalent to active transport, the intracellular NaCl transport pool, the rate of active NaCl transport, and the flux of NaCl into the cell across the apical membrane. Possible future applications of optical techniques include measurement of water permeability, solute reflection coefficients, fluid flow paterns, and interspace osmolality."}
{"id": "PMID:474757", "title": "Resistance to the phosphaturic effect of parathyroid hormone in the hamster.", "content": "The renal effects of parathyroid hormone (PTH) and dibutyryl 3'5'-cyclic AMP (DBcAMP) were studied in thyroparathyroidectomized hamsters. The hamsters were permitted free access to food and water or fasted for 16 h. PTH caused a phosphaturia in the fed hamster (fractional excretion of phosphate (FEPO4) increased from 5.8 +/- 1.3 to 27.4 +/- 4.6%, P less than 0.001) but not in the fasted hamster (from 9.9 +/- 2.5 to 12.4 +/- 2.5%, NS), whereas calcium excretion decreased significantly in both groups. There was no significant difference in blood acid-base or phosphate levels between the two groups. Insulin did not restore the phosphaturic response to PTH (FEPO4 from 7.7 +/- 2.6 to 5.3 +/- 1.7%), whereas phosphate or NH4Cl infusion did, FEPO4 increasing from 20.9 +/- 3.1 to 38.1 +/- 5.4% (P less than 0.02) and from 19.5 +/- 3.8 to 39.0 +/- 7.5%, respectively. DBcAMP caused a phosphaturia both in the fasted (from 9.6 +/- 2.7 to 20.1 +/- 4.5%, P less than 0.01) and fed (from 2.5 +/- 0.5 to 10.7 +/- 1.5%, P less than 0.02) hamster. A fasting state of up to 64 h did not produce resistance to PTH in the rat. It is concluded that fasting produces resistance to the phosphaturic but not the calcium-retaining effects of PTH in the hamster. The resistance may occur, at least partly, prior to the production of cAMP within the renal tubular cell.", "contents": "Resistance to the phosphaturic effect of parathyroid hormone in the hamster. The renal effects of parathyroid hormone (PTH) and dibutyryl 3'5'-cyclic AMP (DBcAMP) were studied in thyroparathyroidectomized hamsters. The hamsters were permitted free access to food and water or fasted for 16 h. PTH caused a phosphaturia in the fed hamster (fractional excretion of phosphate (FEPO4) increased from 5.8 +/- 1.3 to 27.4 +/- 4.6%, P less than 0.001) but not in the fasted hamster (from 9.9 +/- 2.5 to 12.4 +/- 2.5%, NS), whereas calcium excretion decreased significantly in both groups. There was no significant difference in blood acid-base or phosphate levels between the two groups. Insulin did not restore the phosphaturic response to PTH (FEPO4 from 7.7 +/- 2.6 to 5.3 +/- 1.7%), whereas phosphate or NH4Cl infusion did, FEPO4 increasing from 20.9 +/- 3.1 to 38.1 +/- 5.4% (P less than 0.02) and from 19.5 +/- 3.8 to 39.0 +/- 7.5%, respectively. DBcAMP caused a phosphaturia both in the fasted (from 9.6 +/- 2.7 to 20.1 +/- 4.5%, P less than 0.01) and fed (from 2.5 +/- 0.5 to 10.7 +/- 1.5%, P less than 0.02) hamster. A fasting state of up to 64 h did not produce resistance to PTH in the rat. It is concluded that fasting produces resistance to the phosphaturic but not the calcium-retaining effects of PTH in the hamster. The resistance may occur, at least partly, prior to the production of cAMP within the renal tubular cell."}
{"id": "PMID:474758", "title": "Contribution of renal prostaglandins to the natriuretic action of bradykinin in the dog.", "content": "To study the effects of stimulation of renal prostaglandin biosynthesis by bradykinin, we assessed the changes in renal functions induced by intrarenal infusion of bradykinin (10 ng . min-1 . kg-1) in the dog anesthetized with pentobarbital before and during inhibition of prostaglandin synthesis by sodium meclofenamate (5 mg/kg). Before meclofenamate administration, bradykinin augmented the urinary output of a \"PGE\"-like substance from 1.00 +/- 0.25 to 3.88 +/- 1.09 ng/min (P less than 0.05) and increased renal blood flow by 65 +/- 9 ml/min (P less than 0.001), urine flow by 0.55 +/- 0.23 ml/min (P less than 0.05), and sodium excretion by 64.8 +/- 18.0 mueq/min (P less than 0.01). Administration of meclofenamate did not affect the bradykinin-induced increase in renal blood flow and urine volume, but suppressed the evoked output of \"PGE\" and reduced the associated natriuresis, i.e., sodium excretion increased by only 11.1 +/- 4.8 mueq/min (P greater than 0.05). In contrast, meclofenamate did not affect the natriuresis effected by an equidilator dose of PGE2 (5 ng . min-1 . kg-1) infused intrarenally. These observations suggest that a product of prostaglandin synthetase produced by the kidney during intrarenal infusion of bradykinin contributes to the natriuretic action of the peptide.", "contents": "Contribution of renal prostaglandins to the natriuretic action of bradykinin in the dog. To study the effects of stimulation of renal prostaglandin biosynthesis by bradykinin, we assessed the changes in renal functions induced by intrarenal infusion of bradykinin (10 ng . min-1 . kg-1) in the dog anesthetized with pentobarbital before and during inhibition of prostaglandin synthesis by sodium meclofenamate (5 mg/kg). Before meclofenamate administration, bradykinin augmented the urinary output of a \"PGE\"-like substance from 1.00 +/- 0.25 to 3.88 +/- 1.09 ng/min (P less than 0.05) and increased renal blood flow by 65 +/- 9 ml/min (P less than 0.001), urine flow by 0.55 +/- 0.23 ml/min (P less than 0.05), and sodium excretion by 64.8 +/- 18.0 mueq/min (P less than 0.01). Administration of meclofenamate did not affect the bradykinin-induced increase in renal blood flow and urine volume, but suppressed the evoked output of \"PGE\" and reduced the associated natriuresis, i.e., sodium excretion increased by only 11.1 +/- 4.8 mueq/min (P greater than 0.05). In contrast, meclofenamate did not affect the natriuresis effected by an equidilator dose of PGE2 (5 ng . min-1 . kg-1) infused intrarenally. These observations suggest that a product of prostaglandin synthetase produced by the kidney during intrarenal infusion of bradykinin contributes to the natriuretic action of the peptide."}
{"id": "PMID:474759", "title": "Regional renin release by the cat kidney in vitro and in vivo.", "content": "Differences in the rate of renin release by superficial and deep areas of the cat kidney cortex were studied in vitro and in vivo. Renin release in vitro by outer cortical slices was significantly higher than by their inner counterparts: 19.6 +/- 2.3 vs. 12.8 +/- 1.95 ng angiotensin I-h-1-mg fresh tissue-1-h of incubation-1. In vivo blood was sampled from subcapsular (outer cortical) and deep (inner cortical and medullary) renal venous circulation from anesthetized cats. Renal venous minus arterial plasma renin concentration was respectively, 4.3 +/- 1.12 and 1.9 +/- 1.04 ng angiotensin I-ml-1-h-1 (P less than 0.01). By assuming that in these experiments renal blood flow distribution was approximately equal to each of the two areas of venous drainage, as reported in the isolated perfused cat kidney, we infer from the regional differences in arteriovenous concentration that the rate of renin release of the outer cortex is higher than that of the innder cortex in the cat kidney in vivo. Tissue content of renin was found to decrease toward the deep cortex. The results support the concept that the rate of regional renin release correlates with tissue renin content, at least under the conditions of the present experiments.", "contents": "Regional renin release by the cat kidney in vitro and in vivo. Differences in the rate of renin release by superficial and deep areas of the cat kidney cortex were studied in vitro and in vivo. Renin release in vitro by outer cortical slices was significantly higher than by their inner counterparts: 19.6 +/- 2.3 vs. 12.8 +/- 1.95 ng angiotensin I-h-1-mg fresh tissue-1-h of incubation-1. In vivo blood was sampled from subcapsular (outer cortical) and deep (inner cortical and medullary) renal venous circulation from anesthetized cats. Renal venous minus arterial plasma renin concentration was respectively, 4.3 +/- 1.12 and 1.9 +/- 1.04 ng angiotensin I-ml-1-h-1 (P less than 0.01). By assuming that in these experiments renal blood flow distribution was approximately equal to each of the two areas of venous drainage, as reported in the isolated perfused cat kidney, we infer from the regional differences in arteriovenous concentration that the rate of renin release of the outer cortex is higher than that of the innder cortex in the cat kidney in vivo. Tissue content of renin was found to decrease toward the deep cortex. The results support the concept that the rate of regional renin release correlates with tissue renin content, at least under the conditions of the present experiments."}
{"id": "PMID:474760", "title": "Effects of lumen volume transit time and pressure on loop of Henle function.", "content": "Experiments on Henle's loops were designed to demonstrate the relationships of absorption to distal pressure, transit time, and luminal diameter. Loops of superficial nephrons in hydropenic rats, isolated from the rest of the nephron by oil or solid paraffin blocks, were microperfused at 13.6--20 nl/min. Two samples of fluid were collected from the early distal tubule--one with suction in order to lower distal pressure and reduce luminal volume, the other without suction so that the lumen was distended. Transit times were 30 +/- 2 s without and 19 +/- 2 s with suction. Proximal tubule pressure and perfusion rate were not altered by collection with suction. Absolute absorption, however, descreased from 10.6 +/- 0.4 to 8.4 +/- 0.4 nl/min (P less than 0.001). When salt transport was inhibited by 10(-4) M furosemide in the perfusate, water absorption was 7.8 +/- 0.7 nl/min without suction and 6.1 +/- 0.8 nl/min with suction (P less than 0.01). Computer simulation of Henle's loop shows that these observations cannot be explained by changes in transit time, hydrostatic pressure, or unstirred layers. The observations are simulated when radial fluxes depend on wall thickness and surface area in the descending thin limb.", "contents": "Effects of lumen volume transit time and pressure on loop of Henle function. Experiments on Henle's loops were designed to demonstrate the relationships of absorption to distal pressure, transit time, and luminal diameter. Loops of superficial nephrons in hydropenic rats, isolated from the rest of the nephron by oil or solid paraffin blocks, were microperfused at 13.6--20 nl/min. Two samples of fluid were collected from the early distal tubule--one with suction in order to lower distal pressure and reduce luminal volume, the other without suction so that the lumen was distended. Transit times were 30 +/- 2 s without and 19 +/- 2 s with suction. Proximal tubule pressure and perfusion rate were not altered by collection with suction. Absolute absorption, however, descreased from 10.6 +/- 0.4 to 8.4 +/- 0.4 nl/min (P less than 0.001). When salt transport was inhibited by 10(-4) M furosemide in the perfusate, water absorption was 7.8 +/- 0.7 nl/min without suction and 6.1 +/- 0.8 nl/min with suction (P less than 0.01). Computer simulation of Henle's loop shows that these observations cannot be explained by changes in transit time, hydrostatic pressure, or unstirred layers. The observations are simulated when radial fluxes depend on wall thickness and surface area in the descending thin limb."}
{"id": "PMID:474762", "title": "Transport and metabolism of octanoate by the perfused rat kidney.", "content": "These studies were done to determine the capacity of the perfused rat kidney to metabolize and transport the medium-chain fatty acid, octanoate (C8). Use of C8, which is water soluble, facilitated the study of transport, since protein, normally needed to transport long-chain fatty acids in solution, could be omitted from the perfusate. Using a filtering kidney it was found that total metabolism and incorporation of [1-14C]octanoate occurred at a rate of 3.9 +/- 0.3 micromol-g wet wt-1-20 min-1 when the perfusate octanoate was 2.8 mM, and reabsorptive uptake occurred at the rate of 7.5 +/- 1.3 micromol-g wet wt-1-20 min-1 at the same octanoate concentration. Use of a nonfiltering kidney (10% dextran perfusate) allowed quantitation of peritubular octanoate uptake. This peritubular uptake showed saturation above 0.7 mM perfusate octanoate with an apparent transport maximum (Tmax) at 2.1 micromol-g wet wt-1-20 min-1. Many previous experiments have linked renal fatty acid transport with that of the organic anion transport system. The apparent peritubular Tmax observed for octanoate suggests carrier-mediated transport. However, this transport did not appear to be inhibited by other organic acids such as probenecid (1--2 mM) and p-aminohippurate (1--2 mM).", "contents": "Transport and metabolism of octanoate by the perfused rat kidney. These studies were done to determine the capacity of the perfused rat kidney to metabolize and transport the medium-chain fatty acid, octanoate (C8). Use of C8, which is water soluble, facilitated the study of transport, since protein, normally needed to transport long-chain fatty acids in solution, could be omitted from the perfusate. Using a filtering kidney it was found that total metabolism and incorporation of [1-14C]octanoate occurred at a rate of 3.9 +/- 0.3 micromol-g wet wt-1-20 min-1 when the perfusate octanoate was 2.8 mM, and reabsorptive uptake occurred at the rate of 7.5 +/- 1.3 micromol-g wet wt-1-20 min-1 at the same octanoate concentration. Use of a nonfiltering kidney (10% dextran perfusate) allowed quantitation of peritubular octanoate uptake. This peritubular uptake showed saturation above 0.7 mM perfusate octanoate with an apparent transport maximum (Tmax) at 2.1 micromol-g wet wt-1-20 min-1. Many previous experiments have linked renal fatty acid transport with that of the organic anion transport system. The apparent peritubular Tmax observed for octanoate suggests carrier-mediated transport. However, this transport did not appear to be inhibited by other organic acids such as probenecid (1--2 mM) and p-aminohippurate (1--2 mM)."}
{"id": "PMID:474763", "title": "Effect of potassium deficiency on papillary plasma flow in the rat.", "content": "Chronic potassium (K+) deficiency has been shown previously to cause a reduction in solute content in the renal papilla, an effect that is potentially important as a contributing factor to the concentrating defect seen in this circumstance. The cause of the decrease in papillary solute content has not been adequately explained. Because alterations in the blood flow rate through the renal papilla may affect the solute content of the papilla, the present experiments examined the effect of chronic K+ deficiency on papillary plasma flow (PPF) in the rat. PPF was measured by the radioactive albumin accumulation technique. Sprague-Dawley rats were fed identical quantities of water and either a normal or a K+-deficient diet for 21 days. Total GRR in the control rats, 1.7 +/- 0.17 (SE) ml/min, was similar to that in K+-deficient rats, 1.4 +/- 0.14 ml/min (P greater than 0.01). Total [3H]PAH clearance was also comparable in the two groups, i.e., 4.4 +/- 0.47 in control and 4.7 +/- 0.45 ml/min in K+-deficient rats (P greater than 0.06). PPF was significantly lower in K+-deficient rats, 19.7 +/- 1.1 ml-min-1-100 g-1, than in control rats, 59.8 +/- 1.6 ml-min-1-100 g-1 (P less than 0.001). The decrease in PPF in the K+-deficient rat may reflect a reduction in perfusion to the juxtamedullary nephrons, thereby resulting in a diminution in both solute delivery and blood flow to the papilla.", "contents": "Effect of potassium deficiency on papillary plasma flow in the rat. Chronic potassium (K+) deficiency has been shown previously to cause a reduction in solute content in the renal papilla, an effect that is potentially important as a contributing factor to the concentrating defect seen in this circumstance. The cause of the decrease in papillary solute content has not been adequately explained. Because alterations in the blood flow rate through the renal papilla may affect the solute content of the papilla, the present experiments examined the effect of chronic K+ deficiency on papillary plasma flow (PPF) in the rat. PPF was measured by the radioactive albumin accumulation technique. Sprague-Dawley rats were fed identical quantities of water and either a normal or a K+-deficient diet for 21 days. Total GRR in the control rats, 1.7 +/- 0.17 (SE) ml/min, was similar to that in K+-deficient rats, 1.4 +/- 0.14 ml/min (P greater than 0.01). Total [3H]PAH clearance was also comparable in the two groups, i.e., 4.4 +/- 0.47 in control and 4.7 +/- 0.45 ml/min in K+-deficient rats (P greater than 0.06). PPF was significantly lower in K+-deficient rats, 19.7 +/- 1.1 ml-min-1-100 g-1, than in control rats, 59.8 +/- 1.6 ml-min-1-100 g-1 (P less than 0.001). The decrease in PPF in the K+-deficient rat may reflect a reduction in perfusion to the juxtamedullary nephrons, thereby resulting in a diminution in both solute delivery and blood flow to the papilla."}
{"id": "PMID:474764", "title": "Acute and chronic effects of vasopressin on blood pressure, electrolytes, and fluid volumes.", "content": "Physiological levels of arginine vasopressin (AVP) were continuously infused 24 h/day into six dogs for periods ranging from 7 to 34 days. The acute and chronic responses of the mean arterial pressure (MAP), body fluid volumes, renal function indices, plasma electrolyte concentrations, plasma renin activity, and urinary electrolyte and water excretion rates were measured. MAP was unaffected acutely but rose significantly to a peak on day 9 before declining toward control. MAP was significantly and positively correlated with the plasma volume, but had a diphasic correlation with the plasma sodium concentration and the change in total body sodium. The plasma sodium concentration reached a relatively stable plateau that was maintained in spite of large changes in total body water. We conclude that AVP produces only a transient hypervolemic hypertension; that AVP is a natriuretic agent, either directly or indirectly, both acutely and chronically; and that chronically it is a more potent controller of the plasma sodium concentration than of the total body water except in extreme cases.", "contents": "Acute and chronic effects of vasopressin on blood pressure, electrolytes, and fluid volumes. Physiological levels of arginine vasopressin (AVP) were continuously infused 24 h/day into six dogs for periods ranging from 7 to 34 days. The acute and chronic responses of the mean arterial pressure (MAP), body fluid volumes, renal function indices, plasma electrolyte concentrations, plasma renin activity, and urinary electrolyte and water excretion rates were measured. MAP was unaffected acutely but rose significantly to a peak on day 9 before declining toward control. MAP was significantly and positively correlated with the plasma volume, but had a diphasic correlation with the plasma sodium concentration and the change in total body sodium. The plasma sodium concentration reached a relatively stable plateau that was maintained in spite of large changes in total body water. We conclude that AVP produces only a transient hypervolemic hypertension; that AVP is a natriuretic agent, either directly or indirectly, both acutely and chronically; and that chronically it is a more potent controller of the plasma sodium concentration than of the total body water except in extreme cases."}
{"id": "PMID:474766", "title": "Effect of hypoxia on mechanical properties of hyperthyroid cat papillary muscle.", "content": "It has been previously established that hyperthyroid myocardium exhibits increased performance under well-oxygenated conditions. To date, it is not known whether hyperthyroid cardiac muscle can maintain this increased performance during hypoxia. The responses of isolated right ventricular papillary muscles from hyperthyroid and euthyroid kittens to hypoxia were compared under isometric conditions at 31 degrees C. Under well-oxygenated conditions, the hyperthyroid cardiac muscle exhibited both an increased contractility and an accelerated rate of relaxation. A similar degree of acute hypoxic stress for 15 min resulted in a greater decrease in contractility in the hyperthyroid compared with the euthyroid papillary muscle as indicated by a greater fall in both peak tension development (2.2 +/- 0.25 from 4.2 +/- 0.2 vs. 0.9 +/- 0.15 from 3.2 +/- 0.4 g/mm2, P less than 0.01) and +dT/dt (12.9 +/- 2.3 from 25 +/- 3 vs. 4.0 +/- 0.6 from 14 +/- 1 g-s-1-mm-2, P less than 0.01). In addition, compared with the euthyroid data, hypoxia resulted in impaired myocardial relaxation in the hyperthyroid cardiac muscle. Thus, the hyperthyroid compared with the euthyroid papillary muscle exhibits both a greater decrease in contractility and an impairment of myocardial relaxation during hypoxia, indicating a greater susceptibility to a given hypoxic stress.", "contents": "Effect of hypoxia on mechanical properties of hyperthyroid cat papillary muscle. It has been previously established that hyperthyroid myocardium exhibits increased performance under well-oxygenated conditions. To date, it is not known whether hyperthyroid cardiac muscle can maintain this increased performance during hypoxia. The responses of isolated right ventricular papillary muscles from hyperthyroid and euthyroid kittens to hypoxia were compared under isometric conditions at 31 degrees C. Under well-oxygenated conditions, the hyperthyroid cardiac muscle exhibited both an increased contractility and an accelerated rate of relaxation. A similar degree of acute hypoxic stress for 15 min resulted in a greater decrease in contractility in the hyperthyroid compared with the euthyroid papillary muscle as indicated by a greater fall in both peak tension development (2.2 +/- 0.25 from 4.2 +/- 0.2 vs. 0.9 +/- 0.15 from 3.2 +/- 0.4 g/mm2, P less than 0.01) and +dT/dt (12.9 +/- 2.3 from 25 +/- 3 vs. 4.0 +/- 0.6 from 14 +/- 1 g-s-1-mm-2, P less than 0.01). In addition, compared with the euthyroid data, hypoxia resulted in impaired myocardial relaxation in the hyperthyroid cardiac muscle. Thus, the hyperthyroid compared with the euthyroid papillary muscle exhibits both a greater decrease in contractility and an impairment of myocardial relaxation during hypoxia, indicating a greater susceptibility to a given hypoxic stress."}
{"id": "PMID:474768", "title": "Plasma catecholamine and blood pressure responses to sympathetic stimulation in pithed rats.", "content": "Sympathetic stimulation in pithed rats elicits increases in blood pressure (BP) and plasma levels of catecholamines. The BP is proportional to the logarithm of the plasma norepinephrine (NE) concentration. Adrenal medullectomy diminishes slightly only the initial phase of the BP responses, whereas bretylium blocks completely the BP response and diminishes by about 70% the increase in plasma NE. Adrenal medullectomy completely prevents the increase in plasma epinephrine (Epi) and diminishes by 30% plasma NE. Plasma levels of NE appear to reflect sympathetic neuronal activity, but the NE at sympathetic nerve endings is responsible for the increase in BP. Epi released from the adrenal medulla may enhance and accelerate the initial BP response, but plays only a minor role after the first 15 s.", "contents": "Plasma catecholamine and blood pressure responses to sympathetic stimulation in pithed rats. Sympathetic stimulation in pithed rats elicits increases in blood pressure (BP) and plasma levels of catecholamines. The BP is proportional to the logarithm of the plasma norepinephrine (NE) concentration. Adrenal medullectomy diminishes slightly only the initial phase of the BP responses, whereas bretylium blocks completely the BP response and diminishes by about 70% the increase in plasma NE. Adrenal medullectomy completely prevents the increase in plasma epinephrine (Epi) and diminishes by 30% plasma NE. Plasma levels of NE appear to reflect sympathetic neuronal activity, but the NE at sympathetic nerve endings is responsible for the increase in BP. Epi released from the adrenal medulla may enhance and accelerate the initial BP response, but plays only a minor role after the first 15 s."}
{"id": "PMID:474769", "title": "Cardiac lymph flow in conscious dogs.", "content": "The cardiac lymphatic duct was cannulated in dogs and the exteriorized cannula allowed chronic collection of lymph during the awake state for as long as 3 wk. The surgical methodology and inherent difficulties in the technique are describ:d. Cardiac lymph flow ranged from 0.45--5.6 ml/h in the control state in 14 dogs. An occluding device and flow probe were placed on the circumflex coronary artery (CFX); ultrasonic segment length crystals were placed in the left ventricular free wall in 4 dogs. Occlusion of the CFX in these conscious dogs caused lymph flow to fall as great as 46% below control during the 1st half-hour. Reperfusion of the occluded vessel caused an increase in lymph flow as great as 67% above control. The effect on cardiac lymph flow was demonstrated for a few select drugs that have known effects on the cardiovascular system. Cardiac lymph flow was altered from control as follows: isoproterenol, 42 +/- 11% increase; RO 2-2985, 118 +/- 8% increase; verapamil, 101 +/- 10% increase; propranolol caused no significant change. The conscious dog with cardiac lymph vessel cannulated should provide a model to further study the complexities of cardiac metabolism and physiology without interference of anesthesia and surgical stress.", "contents": "Cardiac lymph flow in conscious dogs. The cardiac lymphatic duct was cannulated in dogs and the exteriorized cannula allowed chronic collection of lymph during the awake state for as long as 3 wk. The surgical methodology and inherent difficulties in the technique are describ:d. Cardiac lymph flow ranged from 0.45--5.6 ml/h in the control state in 14 dogs. An occluding device and flow probe were placed on the circumflex coronary artery (CFX); ultrasonic segment length crystals were placed in the left ventricular free wall in 4 dogs. Occlusion of the CFX in these conscious dogs caused lymph flow to fall as great as 46% below control during the 1st half-hour. Reperfusion of the occluded vessel caused an increase in lymph flow as great as 67% above control. The effect on cardiac lymph flow was demonstrated for a few select drugs that have known effects on the cardiovascular system. Cardiac lymph flow was altered from control as follows: isoproterenol, 42 +/- 11% increase; RO 2-2985, 118 +/- 8% increase; verapamil, 101 +/- 10% increase; propranolol caused no significant change. The conscious dog with cardiac lymph vessel cannulated should provide a model to further study the complexities of cardiac metabolism and physiology without interference of anesthesia and surgical stress."}
{"id": "PMID:474770", "title": "Inotropic responsiveness of atrial myocardium bathed in Tris- or bicarbonate-buffered solutions.", "content": "Atrial muscle of guinea pigs was used to study the inotropic influences of substituting a tris(hydroxymethyl)-aminomethane-buffered solution aerated with 100% O2 for a bicarbonate-buffered solution aerated with 95% O2-5% CO2 under otherwise equivalent in vitro conditions. Basal values of isometric contractile tension and its first derivative (dT/dt) were smaller and time to peak tension and time to 90% relaxation were longer in Tris- than in bicarbonate-bathed muscles. Both groups responded similarly to changes in stimulation frequently (0.1--2.2 Hz) and Ca2+ concentration (1.0--7.0 mM); however, maximal inotropic responses to these variables were smaller in the Tris-bathed atria. The negative inotropic effects of D600 and gentamicin were greater in the Tris group. Tris-bathed atria developed pulsus alternans when exposed to Mn2+ or a reduced Ca2+ concentration, whereas pulsus alternans did not occur in the bicarbonate group. A transient increase in contractility occurred in bicarbonate-bathed atria after treatment with 0.125 mM Mn2+, but only a negative response occurred in the Tris group. Thus important, and seemingly Ca2+-dependent, differences exist between the inotropic influences of Tris and bicarbonate solutions that may affect the utility of Tris-buffered (and/or bicarbonate deprived) heart muscle for studying certain inotropic interventions.", "contents": "Inotropic responsiveness of atrial myocardium bathed in Tris- or bicarbonate-buffered solutions. Atrial muscle of guinea pigs was used to study the inotropic influences of substituting a tris(hydroxymethyl)-aminomethane-buffered solution aerated with 100% O2 for a bicarbonate-buffered solution aerated with 95% O2-5% CO2 under otherwise equivalent in vitro conditions. Basal values of isometric contractile tension and its first derivative (dT/dt) were smaller and time to peak tension and time to 90% relaxation were longer in Tris- than in bicarbonate-bathed muscles. Both groups responded similarly to changes in stimulation frequently (0.1--2.2 Hz) and Ca2+ concentration (1.0--7.0 mM); however, maximal inotropic responses to these variables were smaller in the Tris-bathed atria. The negative inotropic effects of D600 and gentamicin were greater in the Tris group. Tris-bathed atria developed pulsus alternans when exposed to Mn2+ or a reduced Ca2+ concentration, whereas pulsus alternans did not occur in the bicarbonate group. A transient increase in contractility occurred in bicarbonate-bathed atria after treatment with 0.125 mM Mn2+, but only a negative response occurred in the Tris group. Thus important, and seemingly Ca2+-dependent, differences exist between the inotropic influences of Tris and bicarbonate solutions that may affect the utility of Tris-buffered (and/or bicarbonate deprived) heart muscle for studying certain inotropic interventions."}
{"id": "PMID:474771", "title": "Uncoupling cation effects on cardiac contractility and sarcolemmal Ca2+ binding.", "content": "Studies have been performed correlating the effects of cationic uncouplers on intact cardiac muscle and on Ca2+ bound to isolated cardiac plasma membranes. Sarcolemmal vesicles were isolated from neonatal rat hearts. Ca2+ binding experiments were performed and Scatchard plot analysis indicated two classes of Ca2+ binding sites. The ability of certain cations to displace Ca2+ bound to these vesicles was measured. The effects of these same cations on the tension developed by neonatal rat papillary muscles and on the Ca2+ content of tissue culture cells (from neonatal rat heart) were measured. The results show that a) the selectivity sequence (Y3+ greater than Nd3+ greater than La3+, Cd2+ greater than Co2+ greater than Mg2+) of sarcolemmal Ca2+ binding sites is the same as the effective uncoupling sequence; b) the amount of Ca2+ bound at these sites (approximately 700 micromol/kg wet wt) is more than adequate to support tension development; c) the dependence of sarcolemmal Ca2+ binding at low-affinity sites and tension development on [Ca2+]o is essentially the same. It is then reasonable to propose that the Ca2+ bound to these sarcolemmal sites plays an important role in controlling the amount of Ca2+ available to the myofilaments and thus myocardial contractility.", "contents": "Uncoupling cation effects on cardiac contractility and sarcolemmal Ca2+ binding. Studies have been performed correlating the effects of cationic uncouplers on intact cardiac muscle and on Ca2+ bound to isolated cardiac plasma membranes. Sarcolemmal vesicles were isolated from neonatal rat hearts. Ca2+ binding experiments were performed and Scatchard plot analysis indicated two classes of Ca2+ binding sites. The ability of certain cations to displace Ca2+ bound to these vesicles was measured. The effects of these same cations on the tension developed by neonatal rat papillary muscles and on the Ca2+ content of tissue culture cells (from neonatal rat heart) were measured. The results show that a) the selectivity sequence (Y3+ greater than Nd3+ greater than La3+, Cd2+ greater than Co2+ greater than Mg2+) of sarcolemmal Ca2+ binding sites is the same as the effective uncoupling sequence; b) the amount of Ca2+ bound at these sites (approximately 700 micromol/kg wet wt) is more than adequate to support tension development; c) the dependence of sarcolemmal Ca2+ binding at low-affinity sites and tension development on [Ca2+]o is essentially the same. It is then reasonable to propose that the Ca2+ bound to these sarcolemmal sites plays an important role in controlling the amount of Ca2+ available to the myofilaments and thus myocardial contractility."}
{"id": "PMID:474772", "title": "Application of an isolated heart model to investigate blood-oxygen delivery.", "content": "To avoid the compensatory hemodynamic responses, which have limited interpretation of hemoglobin-oxygen affinity modifications in animal experimentation, an isolated blood-perfused rabbit heart model providing metabolic, functional, and vectorcardiographic measurements has been developed. Fixed-flow perfusions of unchanged or affinity-modified red blood cell suspensions were carried out to assess the benefits of high affinity during hypoxic hypoxia and of low affinity during posthypoxic recovery. Using fully saturated suspensions, the influence of affinity level during restricted flow and reperfusion was also studied. Higher myocardial oxygen consumption (MVO2) was associated with high-affinity blood during mild hypoxia and low-affinity blood during posthypoxic recovery. At low flows, heart rate and MVO2 tended to be lower in high-affinity perfusions, and to recover more completely during low-affinity reperfusions. Ventricular function, vectorcardiographic patterns, and lactate levels were affected by hypoxia and ischemia, but not by level of affinity. The relevance of these observations to the therapeutic potential of hemoglobin-oxygen affinity modification is discussed.", "contents": "Application of an isolated heart model to investigate blood-oxygen delivery. To avoid the compensatory hemodynamic responses, which have limited interpretation of hemoglobin-oxygen affinity modifications in animal experimentation, an isolated blood-perfused rabbit heart model providing metabolic, functional, and vectorcardiographic measurements has been developed. Fixed-flow perfusions of unchanged or affinity-modified red blood cell suspensions were carried out to assess the benefits of high affinity during hypoxic hypoxia and of low affinity during posthypoxic recovery. Using fully saturated suspensions, the influence of affinity level during restricted flow and reperfusion was also studied. Higher myocardial oxygen consumption (MVO2) was associated with high-affinity blood during mild hypoxia and low-affinity blood during posthypoxic recovery. At low flows, heart rate and MVO2 tended to be lower in high-affinity perfusions, and to recover more completely during low-affinity reperfusions. Ventricular function, vectorcardiographic patterns, and lactate levels were affected by hypoxia and ischemia, but not by level of affinity. The relevance of these observations to the therapeutic potential of hemoglobin-oxygen affinity modification is discussed."}
{"id": "PMID:474773", "title": "Interaction of thrombin with mammalian platelets.", "content": "In this study we have explored the interaction of thrombin with platelets from human and rat. Compared to human platelets, rat platelets suspended in plasma required a higher concentration of thrombin for aggregation. This difference in sensitivity to thrombin was maintained when platelets were washed and suspended in buffer. Platelets from both mammals bound thrombin and showed a similar number of binding sites. However, the apparent dissociation constant of thrombin binding for rat platelets was approximately 15-fold higher than that for human. Thus, the decreased aggregation response of rat platelets may be due to a reduced binding of thrombin to its receptor. It is known that the thrombin receptor is located on the platelet surface. Gel electrophoresis of platelets followed by specific staining as well as fluorography showed significant differences in the surface glycoproteins of human and rat platelets. Human platelets showed labeled components corresponding to 210,000 and 160,000 daltons, whereas rat platelets showed glycoproteins with molecular weights of 240,000 and 190,000. A 135,000-dalton component was present in platelets from both sources. These results suggest that either or both glycoproteins of 210,000 and 160,000 daltons may be involved in the interaction of thrombin with human platelets.", "contents": "Interaction of thrombin with mammalian platelets. In this study we have explored the interaction of thrombin with platelets from human and rat. Compared to human platelets, rat platelets suspended in plasma required a higher concentration of thrombin for aggregation. This difference in sensitivity to thrombin was maintained when platelets were washed and suspended in buffer. Platelets from both mammals bound thrombin and showed a similar number of binding sites. However, the apparent dissociation constant of thrombin binding for rat platelets was approximately 15-fold higher than that for human. Thus, the decreased aggregation response of rat platelets may be due to a reduced binding of thrombin to its receptor. It is known that the thrombin receptor is located on the platelet surface. Gel electrophoresis of platelets followed by specific staining as well as fluorography showed significant differences in the surface glycoproteins of human and rat platelets. Human platelets showed labeled components corresponding to 210,000 and 160,000 daltons, whereas rat platelets showed glycoproteins with molecular weights of 240,000 and 190,000. A 135,000-dalton component was present in platelets from both sources. These results suggest that either or both glycoproteins of 210,000 and 160,000 daltons may be involved in the interaction of thrombin with human platelets."}
{"id": "PMID:474774", "title": "Aortic nerve activation: effect of stimulus train composition on heart rate.", "content": "The central end of the sectioned left aortic nerve was stimulated within one heart cycle by a train of electrical pulses, producing a reflex reduction in heart rate in the anesthetized rabbit. Relative importance of the stimulation variables (train duration, pulse frequency, and pulse number) in producing bradycardia was investigated by maintaining one variable constant, while altering the other two over a wide range. At each fixed pulse frequency, maximal bradycardia was obtained by the longest train duration that corresponded with the greatest pulse number. At each fixed train duration, maximal bradycardia was achieved with pulse frequencies between 80--120 Hz and remained maximal up to 160 Hz. Stimulating over wide variations in train duration and pulse frequency at any fixed pulse number produced no significant differences in heart rate reduction at that pulse number. These results indicate the dominant importance of total pulse number per cardiac cycle in determining the magnitude of the heart rate response and reveal an apparent frequency limitation within the reflex.", "contents": "Aortic nerve activation: effect of stimulus train composition on heart rate. The central end of the sectioned left aortic nerve was stimulated within one heart cycle by a train of electrical pulses, producing a reflex reduction in heart rate in the anesthetized rabbit. Relative importance of the stimulation variables (train duration, pulse frequency, and pulse number) in producing bradycardia was investigated by maintaining one variable constant, while altering the other two over a wide range. At each fixed pulse frequency, maximal bradycardia was obtained by the longest train duration that corresponded with the greatest pulse number. At each fixed train duration, maximal bradycardia was achieved with pulse frequencies between 80--120 Hz and remained maximal up to 160 Hz. Stimulating over wide variations in train duration and pulse frequency at any fixed pulse number produced no significant differences in heart rate reduction at that pulse number. These results indicate the dominant importance of total pulse number per cardiac cycle in determining the magnitude of the heart rate response and reveal an apparent frequency limitation within the reflex."}
{"id": "PMID:474775", "title": "Early changes in collateral blood flow during myocardial infarction in conscious dogs.", "content": "We studied the early changes in collateral blood flow (CBF) after acute coronary artery occlusion and the relation of these changes to subsequent necrosis. We measured CBF with 7--9 microns radioactive microspheres before and at various times after circumflex artery occlusion in 42 conscious dogs that were killed 48 h later. CBF increased from 20 s postocclusion to later measurements (5 min, 15 min, 1 h, or 6 h) and did so in both necrotic and nonnecrotic areas of the occluded bed. However, the increase in CBF over time was not gradual, but appeared to occur between 20 s and 5 min, with no further changes for up to 6 h. There was a gradation of CBF in the occluded bed, from periphery to center and subepicardium to subendocardium. Central and subendocardial regions with CBF less than 0.40 ml-min-1-g-1 at 5--15 min postocclusion subsequently showed necrosis whereas epicardial and lateral regions with CBF greater than 0.50 ml/min did not. Thus CBF increases very early throughout the occluded coronary bed, and the level of CBF by 5 min appears to determine whether necrosis ultimately occurs.", "contents": "Early changes in collateral blood flow during myocardial infarction in conscious dogs. We studied the early changes in collateral blood flow (CBF) after acute coronary artery occlusion and the relation of these changes to subsequent necrosis. We measured CBF with 7--9 microns radioactive microspheres before and at various times after circumflex artery occlusion in 42 conscious dogs that were killed 48 h later. CBF increased from 20 s postocclusion to later measurements (5 min, 15 min, 1 h, or 6 h) and did so in both necrotic and nonnecrotic areas of the occluded bed. However, the increase in CBF over time was not gradual, but appeared to occur between 20 s and 5 min, with no further changes for up to 6 h. There was a gradation of CBF in the occluded bed, from periphery to center and subepicardium to subendocardium. Central and subendocardial regions with CBF less than 0.40 ml-min-1-g-1 at 5--15 min postocclusion subsequently showed necrosis whereas epicardial and lateral regions with CBF greater than 0.50 ml/min did not. Thus CBF increases very early throughout the occluded coronary bed, and the level of CBF by 5 min appears to determine whether necrosis ultimately occurs."}
{"id": "PMID:474776", "title": "Vascular responses in canine subcutaneous adipose tissue to hypothalamic stimulation.", "content": "The present studies investigated vascular responses to electrical stimulation of the posterior hypothalamus in isolated but perfused and innervated subcutaneous adipose tissue in adult dogs. Three groups of dogs were distinguished: in one, electrical stimulation elicited vasodilation; in another, vasoconstriction, and in a third, neither significant vasodilation nor vasoconstriction occurred. Histological examination revealed that electrode placements were in the medial posterior hypothalamus, the lateral posterior hypothalamus, and the medial septal region, respectively. Hypothalamic stimulation failed to alter concentrations of free fatty acids or glycerol in venous blood from subcutaneous fat. Local beta-adrenergic block (propranolol) reversed the vasodilation to vasoconstriction while local alpha-adrenergic block (dihydroergotamine) abolished the vaso constrictor response. These results suggest that selective stimulation of the posterior hypothalamus results in neurogenic activation of both alpha- and beta-adrenergic mechanisms in adipose tissue vasculature. beta-Adrenergic vasodilation appears to predominate if the electrode is located medially, and alpha-adrenergic vasoconstriction appears to predominate if the electrode is located in the lateral posterior hypothalamus.", "contents": "Vascular responses in canine subcutaneous adipose tissue to hypothalamic stimulation. The present studies investigated vascular responses to electrical stimulation of the posterior hypothalamus in isolated but perfused and innervated subcutaneous adipose tissue in adult dogs. Three groups of dogs were distinguished: in one, electrical stimulation elicited vasodilation; in another, vasoconstriction, and in a third, neither significant vasodilation nor vasoconstriction occurred. Histological examination revealed that electrode placements were in the medial posterior hypothalamus, the lateral posterior hypothalamus, and the medial septal region, respectively. Hypothalamic stimulation failed to alter concentrations of free fatty acids or glycerol in venous blood from subcutaneous fat. Local beta-adrenergic block (propranolol) reversed the vasodilation to vasoconstriction while local alpha-adrenergic block (dihydroergotamine) abolished the vaso constrictor response. These results suggest that selective stimulation of the posterior hypothalamus results in neurogenic activation of both alpha- and beta-adrenergic mechanisms in adipose tissue vasculature. beta-Adrenergic vasodilation appears to predominate if the electrode is located medially, and alpha-adrenergic vasoconstriction appears to predominate if the electrode is located in the lateral posterior hypothalamus."}
{"id": "PMID:474777", "title": "Intracellular recording from beating heart in situ using a special micropipette holder.", "content": "Use of a motion-compensated micropipette holder, which senses cardiac motion and lifts and lowers the micropipette and supporting apparatus in synchrony with the heart beat, allows for stable recording of transmembrane action potentials from subepicardial cells of an in vivo beating heart in an open-chest dog without significantly impairing cardiac hemodynamics. This technique may be used to study the effects of ischemia, hypertrophy, or pharmacologic agents on the cellular electrophysiological parameters of subepicardial ventricular cells of an in vivo beating heart.", "contents": "Intracellular recording from beating heart in situ using a special micropipette holder. Use of a motion-compensated micropipette holder, which senses cardiac motion and lifts and lowers the micropipette and supporting apparatus in synchrony with the heart beat, allows for stable recording of transmembrane action potentials from subepicardial cells of an in vivo beating heart in an open-chest dog without significantly impairing cardiac hemodynamics. This technique may be used to study the effects of ischemia, hypertrophy, or pharmacologic agents on the cellular electrophysiological parameters of subepicardial ventricular cells of an in vivo beating heart."}
{"id": "PMID:474778", "title": "On-line analysis of intracellular electrophysiological data using a microcomputer system.", "content": "Automated analysis of intracellular action potentials from cardiac Purkinje fibers was implemented using a microcomputer system. A dual sampling rate was used during analog-to-digital conversion of action potentials recorded from stimulated fibers. The rapid phase of depolarization was sampled at 42.55 kHz. The repolarization and the diastolic phases were sampled at 1 kHz. The resting potential, action potential amplitude, conduction time, action potential duration measured at 50% and at 90% of repolarization, and the maximum upstroke velocity were obtained on-line. The digital form of the action potential was stored on cassette tape and a table containing the various measurements was assembled during the experiment. In unstimulated fibers, the time interval between consecutive action potentials was measured on-line along with the maximum diastolic potential, the action potential overshoot, and the slope of the diastolic depolarization.", "contents": "On-line analysis of intracellular electrophysiological data using a microcomputer system. Automated analysis of intracellular action potentials from cardiac Purkinje fibers was implemented using a microcomputer system. A dual sampling rate was used during analog-to-digital conversion of action potentials recorded from stimulated fibers. The rapid phase of depolarization was sampled at 42.55 kHz. The repolarization and the diastolic phases were sampled at 1 kHz. The resting potential, action potential amplitude, conduction time, action potential duration measured at 50% and at 90% of repolarization, and the maximum upstroke velocity were obtained on-line. The digital form of the action potential was stored on cassette tape and a table containing the various measurements was assembled during the experiment. In unstimulated fibers, the time interval between consecutive action potentials was measured on-line along with the maximum diastolic potential, the action potential overshoot, and the slope of the diastolic depolarization."}
{"id": "PMID:474781", "title": "Forum on osmosis. II. A criticism of \"solvent tension\" in osmosis.", "content": "Concepts about the liquid state that are inconsistent with the facts are noted, and solvent under tension is one of them. Osmosis is not present in a solution: it is a process in the presence of a semipermeable membrane that can be quantified by the operation of applying a hydrostatic pressure. The classical derivation of the van't Hoff equation from Raoult's law is reviewed. The soundness of the early views of G. H. Lewis has not changed.", "contents": "Forum on osmosis. II. A criticism of \"solvent tension\" in osmosis. Concepts about the liquid state that are inconsistent with the facts are noted, and solvent under tension is one of them. Osmosis is not present in a solution: it is a process in the presence of a semipermeable membrane that can be quantified by the operation of applying a hydrostatic pressure. The classical derivation of the van't Hoff equation from Raoult's law is reviewed. The soundness of the early views of G. H. Lewis has not changed."}
{"id": "PMID:474783", "title": "Forum on osmosis. IV. More on osmosis and diffusion.", "content": "A brief summary is presented of the Gibbsian view of chemical drives and of its mechanical interpretation, including a description of the \"diffusion force\" that arises from an interplay between fluctuations and dissipation. Osmotic flows are shown to be driven by diffusion forces acting at the membrane interface, and not by the effects of Hammel and Scholander's \"solvent tension.\"", "contents": "Forum on osmosis. IV. More on osmosis and diffusion. A brief summary is presented of the Gibbsian view of chemical drives and of its mechanical interpretation, including a description of the \"diffusion force\" that arises from an interplay between fluctuations and dissipation. Osmotic flows are shown to be driven by diffusion forces acting at the membrane interface, and not by the effects of Hammel and Scholander's \"solvent tension.\""}
{"id": "PMID:474785", "title": "Oral motor deficits following lesions of the central nervous system in the rat.", "content": "The effects of lesions of the zona incerta (ZI), globus pallidus (GP), midlateral and far lateral hypothalamus (MLH and FLH), and the central amygdaloid complex (CAC) on oral motor deficits were investigated. Lesions of the ZI, GP, and CAC resulted in a significant reduction in tongue extension and lap volume. MLH lesions significantly reduced tongue extension whereas FLH lesions significantly reduced both tongue extension and lap volume. Injections of 6-hydroxydopamine into the GP, MLH, and CAC also significantly reduced tongue extension and lap volume. Water and/or food intakes were reduced in some of the lesioned groups but the oral motor deficits were observed both in the presence and in the absence of reduced water and food intakes. The possibility that oral motor deficits are associated with damage to striatal and non-striatal dopamine neurons needs further investigation.", "contents": "Oral motor deficits following lesions of the central nervous system in the rat. The effects of lesions of the zona incerta (ZI), globus pallidus (GP), midlateral and far lateral hypothalamus (MLH and FLH), and the central amygdaloid complex (CAC) on oral motor deficits were investigated. Lesions of the ZI, GP, and CAC resulted in a significant reduction in tongue extension and lap volume. MLH lesions significantly reduced tongue extension whereas FLH lesions significantly reduced both tongue extension and lap volume. Injections of 6-hydroxydopamine into the GP, MLH, and CAC also significantly reduced tongue extension and lap volume. Water and/or food intakes were reduced in some of the lesioned groups but the oral motor deficits were observed both in the presence and in the absence of reduced water and food intakes. The possibility that oral motor deficits are associated with damage to striatal and non-striatal dopamine neurons needs further investigation."}
{"id": "PMID:474787", "title": "Computer simulation of metabolism in pyruvate-perfused rat heart. I. Model construction.", "content": "A computer model of energy metabolism was constructed for the pyruvate-perfused rat heart subjected to a sudden increase in work load. The model construction techniques are explained and justified. Hypotheses defining a plausible sequence of physiological events resulting in the observed behavior of the organ were arrived at from the measured metabolite time profiles. There is a lag in increasing pyruvate utilization following the work-jump. A transient increase in fatty acid oxidation is required to account for the observed respiration rate. The observed increase in lactate production of this preparation is due to a transient \"burst\" of glycogenolysis after which the remaining lactate is imported from the interstitium and oxidized, reversing lactate dehydrogenase and hence the alpha-ketoglutarate-malate portion of the malate-aspartate shuttle.", "contents": "Computer simulation of metabolism in pyruvate-perfused rat heart. I. Model construction. A computer model of energy metabolism was constructed for the pyruvate-perfused rat heart subjected to a sudden increase in work load. The model construction techniques are explained and justified. Hypotheses defining a plausible sequence of physiological events resulting in the observed behavior of the organ were arrived at from the measured metabolite time profiles. There is a lag in increasing pyruvate utilization following the work-jump. A transient increase in fatty acid oxidation is required to account for the observed respiration rate. The observed increase in lactate production of this preparation is due to a transient \"burst\" of glycogenolysis after which the remaining lactate is imported from the interstitium and oxidized, reversing lactate dehydrogenase and hence the alpha-ketoglutarate-malate portion of the malate-aspartate shuttle."}
{"id": "PMID:474788", "title": "Computer simulation of metabolism in pyruvate-perfused rat heart. III. Pyruvate dehydrogenase.", "content": "A physiologically and biochemically realistic model of the regulation of pyruvate dehydrogenase complex (PDH) was constructed for the perfused rat heart. It includes conversion between inactive (phospho) and active (dephospho) forms by a specific protein kinase (PDHK) and phosphoprotein phosphatase (PDHP). The activity of the tightly bound PDHK is influenced by synergistic activation/inhibition by acetyl CoA/CoASH and NADH/NAD. PDHK in this simulation was more sensitive to the fraction of ADP that was Mg2+-chelated than to the ATP-to-ADP ratio. Ca2+ stimulates binding of Mg2+-dependent PDHP to the complex; the bound enzyme was considered to be the active species. The fraction of PDH in the active form, rather than substrate and inhibitor levels, determines PDH activity under these conditions. This fraction depends on the present value and recent history of the difference between PDHK and PDHP activities. Both of these are active continuously and continuously control PDH.", "contents": "Computer simulation of metabolism in pyruvate-perfused rat heart. III. Pyruvate dehydrogenase. A physiologically and biochemically realistic model of the regulation of pyruvate dehydrogenase complex (PDH) was constructed for the perfused rat heart. It includes conversion between inactive (phospho) and active (dephospho) forms by a specific protein kinase (PDHK) and phosphoprotein phosphatase (PDHP). The activity of the tightly bound PDHK is influenced by synergistic activation/inhibition by acetyl CoA/CoASH and NADH/NAD. PDHK in this simulation was more sensitive to the fraction of ADP that was Mg2+-chelated than to the ATP-to-ADP ratio. Ca2+ stimulates binding of Mg2+-dependent PDHP to the complex; the bound enzyme was considered to be the active species. The fraction of PDH in the active form, rather than substrate and inhibitor levels, determines PDH activity under these conditions. This fraction depends on the present value and recent history of the difference between PDHK and PDHP activities. Both of these are active continuously and continuously control PDH."}
{"id": "PMID:474789", "title": "Computer simulation of metabolism in pyruvate-perfused rat heart. IV. Model behavior.", "content": "The behavior of a computer model of energy metabolism was determined for perfused rat hearts utilizing pyruvate as sole exogenous fuel and subjected to a rapid increase in work load. Computer-generated metabolite profiles, which are solutions of the differential equations for 1 min elapsed time, closely match 12 experimental curves (involving 120 concentration measurements) and exhibit the following properties. The computed cytosolic pyruvate level oscillates due to large changes in the rates of the processes that produce and consume this metabolite. Cytosolic Mg2+ seems to act as a coordinated controller of glycolytic enzymes; its transient increase permits a transient increase of glycolysis without an accumulation of glucose 6-phosphate. Lactate is exported to the interstitium by a lactate permease and then reimported and oxidized. As a result, the malate-aspartate shuttle reverses direction, and the Krebs cycle is \"unspanned.\"", "contents": "Computer simulation of metabolism in pyruvate-perfused rat heart. IV. Model behavior. The behavior of a computer model of energy metabolism was determined for perfused rat hearts utilizing pyruvate as sole exogenous fuel and subjected to a rapid increase in work load. Computer-generated metabolite profiles, which are solutions of the differential equations for 1 min elapsed time, closely match 12 experimental curves (involving 120 concentration measurements) and exhibit the following properties. The computed cytosolic pyruvate level oscillates due to large changes in the rates of the processes that produce and consume this metabolite. Cytosolic Mg2+ seems to act as a coordinated controller of glycolytic enzymes; its transient increase permits a transient increase of glycolysis without an accumulation of glucose 6-phosphate. Lactate is exported to the interstitium by a lactate permease and then reimported and oxidized. As a result, the malate-aspartate shuttle reverses direction, and the Krebs cycle is \"unspanned.\""}
{"id": "PMID:474790", "title": "Ontogeny of feeding controls in suckling and weanling rats.", "content": "To rationalize conflicting data in the literature, the latency of attachment of fed and fasted rat pups to the nipples of their anesthetized mother has been studied at various postnatal ages. At 10 and 14 days of age, the mode of fasting had a critical effect on the results: pups fasted in a 30 degrees C incubator had shorter attachment latencies than fed pups, whereas when pups were fasted in a 34 degrees C incubator or with a nonlactating foster mother at room temperature, their latencies were no different from those of fed pups. Thus maternal deprivation has an important influence on attachment latencies, unless other steps are taken to maintain the body temperature of fasted pups. When this variable is controlled, it is apparent that at 10 and 14 days of age, the feeding behavior of rat pups is not related to nutritional status. In contrast, from 17 days onward, latencies of fed pups were significantly longer than those of faster pups, regardless of the mode of fasting. This appearance of appetitive behavior during the 3rd postnatal wk was shown to have a temporal correlation with the beginning of weaning, as measured by the appearance of chow in the stomachs.", "contents": "Ontogeny of feeding controls in suckling and weanling rats. To rationalize conflicting data in the literature, the latency of attachment of fed and fasted rat pups to the nipples of their anesthetized mother has been studied at various postnatal ages. At 10 and 14 days of age, the mode of fasting had a critical effect on the results: pups fasted in a 30 degrees C incubator had shorter attachment latencies than fed pups, whereas when pups were fasted in a 34 degrees C incubator or with a nonlactating foster mother at room temperature, their latencies were no different from those of fed pups. Thus maternal deprivation has an important influence on attachment latencies, unless other steps are taken to maintain the body temperature of fasted pups. When this variable is controlled, it is apparent that at 10 and 14 days of age, the feeding behavior of rat pups is not related to nutritional status. In contrast, from 17 days onward, latencies of fed pups were significantly longer than those of faster pups, regardless of the mode of fasting. This appearance of appetitive behavior during the 3rd postnatal wk was shown to have a temporal correlation with the beginning of weaning, as measured by the appearance of chow in the stomachs."}
{"id": "PMID:474792", "title": "Regulation of cardiac output during rapid volume loading.", "content": "Interactions between heart rate (HR) and the Frank-Starling mechanism in augmenting cardiac output (CO) during acute volume loading were studied in dogs under varying conditions. In normal conscious dogs with low HRs of 70--90 beats/min, end-diastolic diameter (EDD) was maximal and CO was increased reflexly by cardioacceleration. By contrast, anesthetized open-chest dogs with high HRs ranging from 140 to 160 beats/min, responded with bradycardia and CO was adjusted solely by increasing stroke volume (SV). In anesthetized open-chest dogs with low HRs, EDD was less than maximal and increases in both HR and SV contributed to augment CO. These data show that both the cardioacceleration reflex and the Frank-Starling mechanism are important determinants of the cardiac response to elevated preload. Although the relative contribution of each control mechanism varies with initial conditions, their interplay extends the range of cardioregulation beyond that attainable by either loop operating alone.", "contents": "Regulation of cardiac output during rapid volume loading. Interactions between heart rate (HR) and the Frank-Starling mechanism in augmenting cardiac output (CO) during acute volume loading were studied in dogs under varying conditions. In normal conscious dogs with low HRs of 70--90 beats/min, end-diastolic diameter (EDD) was maximal and CO was increased reflexly by cardioacceleration. By contrast, anesthetized open-chest dogs with high HRs ranging from 140 to 160 beats/min, responded with bradycardia and CO was adjusted solely by increasing stroke volume (SV). In anesthetized open-chest dogs with low HRs, EDD was less than maximal and increases in both HR and SV contributed to augment CO. These data show that both the cardioacceleration reflex and the Frank-Starling mechanism are important determinants of the cardiac response to elevated preload. Although the relative contribution of each control mechanism varies with initial conditions, their interplay extends the range of cardioregulation beyond that attainable by either loop operating alone."}
{"id": "PMID:474793", "title": "Sinovagal interaction in arterial pressure restoration after 10% hemorrhage.", "content": "The summation between the carotid sinus baroreceptor reflex system (CS system) and the vagally mediated reflex system (V system) was studied as they restore mean arterial pressure (MAP) after 10% quick hemorrhage in splenectomized conscious dogs chronically instrumented with catheters for pressure measurement and hemorrhage. The experiment was repeated under nerve-intact condition (intact), with cold block of the vagi ([V]), after carotid sinus denervation (CS), and CS plus [V] situations. MAP falls at 1.5 min after the hemorrhage were 7.2 in intact, 24.7 in [V], 36.0 in CS, and 67.6 in CS + [V] mmHg. When we calculated the open-loop gains of CS and V systems assuming a simply additive summation between them a self-contradiction occurred. To avoid this contradiction, it was necessary to assume that CS and V systems interact in a facilitatory manner. Mean open-loop gains calculated under this assumption were 1.64 for the CS system alone, 0.89 for the V system alone, and 6.59 for the interacting component between them. These intriguing results warrant further analysis of the summation between the two reflex systems.", "contents": "Sinovagal interaction in arterial pressure restoration after 10% hemorrhage. The summation between the carotid sinus baroreceptor reflex system (CS system) and the vagally mediated reflex system (V system) was studied as they restore mean arterial pressure (MAP) after 10% quick hemorrhage in splenectomized conscious dogs chronically instrumented with catheters for pressure measurement and hemorrhage. The experiment was repeated under nerve-intact condition (intact), with cold block of the vagi ([V]), after carotid sinus denervation (CS), and CS plus [V] situations. MAP falls at 1.5 min after the hemorrhage were 7.2 in intact, 24.7 in [V], 36.0 in CS, and 67.6 in CS + [V] mmHg. When we calculated the open-loop gains of CS and V systems assuming a simply additive summation between them a self-contradiction occurred. To avoid this contradiction, it was necessary to assume that CS and V systems interact in a facilitatory manner. Mean open-loop gains calculated under this assumption were 1.64 for the CS system alone, 0.89 for the V system alone, and 6.59 for the interacting component between them. These intriguing results warrant further analysis of the summation between the two reflex systems."}
{"id": "PMID:474794", "title": "Effects of jejunoileal bypass on meal patterns in genetically obese and lean rats.", "content": "Jejunoileal bypass surgery, designed to reproduce the human operation (long bypass) was contrasted with a short bypass of only a few centimeters of ileum in both genetically obese rats and their lean littermates. In comparison with the short-bypassed rats, long-bypassed rats of both genotypes lost weight and reduced food intake. When adjustments were made for group differences before surgery, no significant differences were detected in the effectiveness of the operation on the two genotypes. The reduction in food intake was accomplished by a decrease in meal size and an increase in intermeal interval. Food intake gradually returned in the long-bypassed rats toward its level in the short-bypassed rats. This increase was accomplished by an increase in meal size alone. Intermeal intervals remained prolonged. In the long-bypassed rats, compared with the short, meal duration was initially elevated and gradually increased more than did meal size, indicating a great slowing of eating rate. Water intake was unaffected, but water-to-food ratio was elevated initially and gradually declined in the long-bypassed rats. Learned food aversion, dehydration, and gastrointestinal and metabolic alterations due to malabsorption are mentioned as possible contributors to the reduction in food intake, but none of these alone can account for all the changes in meal pattern by which intake reduction is accomplished.", "contents": "Effects of jejunoileal bypass on meal patterns in genetically obese and lean rats. Jejunoileal bypass surgery, designed to reproduce the human operation (long bypass) was contrasted with a short bypass of only a few centimeters of ileum in both genetically obese rats and their lean littermates. In comparison with the short-bypassed rats, long-bypassed rats of both genotypes lost weight and reduced food intake. When adjustments were made for group differences before surgery, no significant differences were detected in the effectiveness of the operation on the two genotypes. The reduction in food intake was accomplished by a decrease in meal size and an increase in intermeal interval. Food intake gradually returned in the long-bypassed rats toward its level in the short-bypassed rats. This increase was accomplished by an increase in meal size alone. Intermeal intervals remained prolonged. In the long-bypassed rats, compared with the short, meal duration was initially elevated and gradually increased more than did meal size, indicating a great slowing of eating rate. Water intake was unaffected, but water-to-food ratio was elevated initially and gradually declined in the long-bypassed rats. Learned food aversion, dehydration, and gastrointestinal and metabolic alterations due to malabsorption are mentioned as possible contributors to the reduction in food intake, but none of these alone can account for all the changes in meal pattern by which intake reduction is accomplished."}
{"id": "PMID:474795", "title": "Rate of generation of myocardial power density normalized by cardiac frequency.", "content": "We introduce a frequency normalized, power averaged, rate of generation of power density (FARPD) that extends the range of applicability of the averaged rate of generation of power density (ARPD) to any cardiac frequency encountered in experimental or clinical situations. Normalization eliminates the frequency-dependent mathematical artifacts arising during the computation of quantities containing time derivatives of the pressure, while preserving the effects of changes in heart rate on the cardiac muscle itself (Bowditch type of phenomena). The distribution of normal values in a group of 51 control dogs was very close to the standard normal distribution. These values had no statistically significant differences with those of a smaller control group studied by independent investigators. Isoproterenol and propranolol produced highly significant statistical changes in FARPD. Alterations in pre- and afterload produced changes that were not significant for the former and significant at the 5% level for the latter. These changes were small when compared to those resulting from typical inotropic interventions.", "contents": "Rate of generation of myocardial power density normalized by cardiac frequency. We introduce a frequency normalized, power averaged, rate of generation of power density (FARPD) that extends the range of applicability of the averaged rate of generation of power density (ARPD) to any cardiac frequency encountered in experimental or clinical situations. Normalization eliminates the frequency-dependent mathematical artifacts arising during the computation of quantities containing time derivatives of the pressure, while preserving the effects of changes in heart rate on the cardiac muscle itself (Bowditch type of phenomena). The distribution of normal values in a group of 51 control dogs was very close to the standard normal distribution. These values had no statistically significant differences with those of a smaller control group studied by independent investigators. Isoproterenol and propranolol produced highly significant statistical changes in FARPD. Alterations in pre- and afterload produced changes that were not significant for the former and significant at the 5% level for the latter. These changes were small when compared to those resulting from typical inotropic interventions."}
{"id": "PMID:474796", "title": "Influence of enteric parasitism on hormone-regulated pancreatic secretion in dogs.", "content": "The objective of this study was to test the hypothesis that enteric parasites affect pancreatic secretion in their host. Pancreatic bicarbonate and protein outputs were studied in dogs with gastric and pancreatic fistulas to determine the secretory response to exogenously administered secretin and cholecystokinin and to intraduodenal stimulation with hydrochloric acid and sodium oleate to release endogenous hormones. Bicarbonate and protein concentrations in pancreatic juice were measured prior to infection with Trichinella spiralis and at various periods during primary and secondary infections. Dose-related increases in secretory activity were observed in uninfected hosts in response to all stimuli. Infected dogs responded like controls to exogenous hormones, but showed reduced secretion under duodenal stimulation during the 1st wk of primary infection. This altered response returned to normal 3 wk after primary infection and did not occur following secondary infection. Results support the conclusion that reduced pancreatic secretion is associated with enteric parasitism and is due to a defect in hormone release or in the supply of hormone available for release.", "contents": "Influence of enteric parasitism on hormone-regulated pancreatic secretion in dogs. The objective of this study was to test the hypothesis that enteric parasites affect pancreatic secretion in their host. Pancreatic bicarbonate and protein outputs were studied in dogs with gastric and pancreatic fistulas to determine the secretory response to exogenously administered secretin and cholecystokinin and to intraduodenal stimulation with hydrochloric acid and sodium oleate to release endogenous hormones. Bicarbonate and protein concentrations in pancreatic juice were measured prior to infection with Trichinella spiralis and at various periods during primary and secondary infections. Dose-related increases in secretory activity were observed in uninfected hosts in response to all stimuli. Infected dogs responded like controls to exogenous hormones, but showed reduced secretion under duodenal stimulation during the 1st wk of primary infection. This altered response returned to normal 3 wk after primary infection and did not occur following secondary infection. Results support the conclusion that reduced pancreatic secretion is associated with enteric parasitism and is due to a defect in hormone release or in the supply of hormone available for release."}
{"id": "PMID:474799", "title": "A commitment law for patients, doctors, and lawyers.", "content": "The author proposes a model commitment law that balances the sometimes conflicting points of view among patients, doctors, and lawyers about this subject. Paternalism is affirmed, while safeguards are provided. It is argued that absent patient incompetency to consent to or refuse treatment, or absent an emergency, mental patients should not be treated involuntarily. The author believes that a different procedural approach is required depending on whether the patient is committed under the parens patriae or police power of the state.", "contents": "A commitment law for patients, doctors, and lawyers. The author proposes a model commitment law that balances the sometimes conflicting points of view among patients, doctors, and lawyers about this subject. Paternalism is affirmed, while safeguards are provided. It is argued that absent patient incompetency to consent to or refuse treatment, or absent an emergency, mental patients should not be treated involuntarily. The author believes that a different procedural approach is required depending on whether the patient is committed under the parens patriae or police power of the state."}
{"id": "PMID:474800", "title": "Violent deaths among the young: recent trends in suicide, homicide, and accidents.", "content": "Violent deaths (suicide, homicide, and accidents) are the leading cause of death in people aged 1--39 in the United States. All three types of violent death may represent suicidal tendencies. Among early (10--14 years), middle (15--19), and late (20--24) adolescents, suicide and homicide rates increased with age and have doubled from 1961 to 1975. Accident rates increased with age but have changed little over the 15-year period. Suicide, homicide, and accidents increased dramatically between the 10--14-year-old age group and the 15--19-year-old age group; suicide and homicide rates were remarkably parallel over time; and suicide rates among young white people were higher and increased more than those among young nonwhite people.", "contents": "Violent deaths among the young: recent trends in suicide, homicide, and accidents. Violent deaths (suicide, homicide, and accidents) are the leading cause of death in people aged 1--39 in the United States. All three types of violent death may represent suicidal tendencies. Among early (10--14 years), middle (15--19), and late (20--24) adolescents, suicide and homicide rates increased with age and have doubled from 1961 to 1975. Accident rates increased with age but have changed little over the 15-year period. Suicide, homicide, and accidents increased dramatically between the 10--14-year-old age group and the 15--19-year-old age group; suicide and homicide rates were remarkably parallel over time; and suicide rates among young white people were higher and increased more than those among young nonwhite people."}
{"id": "PMID:474801", "title": "Analgesia to painful stimuli in affective illness.", "content": "Patients with bipolar and unipolar affective illness (N = 76) were compared with 48 control subjects on a psychophysical pain rating procedure using both threshold and signal detection analysis. Affectively ill patients were more analgesic than controls, and depressed men were significantly more analgesic than depressed women or control subjects. Bipolar men showed a different pattern of analgesia than unipolar patients. Pain appreciation in depressed patients may be related to endogenous opiate-like substances; this could be assessed in narcotic antagonist studies of pain-tolerant depressed subjects.", "contents": "Analgesia to painful stimuli in affective illness. Patients with bipolar and unipolar affective illness (N = 76) were compared with 48 control subjects on a psychophysical pain rating procedure using both threshold and signal detection analysis. Affectively ill patients were more analgesic than controls, and depressed men were significantly more analgesic than depressed women or control subjects. Bipolar men showed a different pattern of analgesia than unipolar patients. Pain appreciation in depressed patients may be related to endogenous opiate-like substances; this could be assessed in narcotic antagonist studies of pain-tolerant depressed subjects."}
{"id": "PMID:474802", "title": "Adults' reactions to the death of a parent: a preliminary study.", "content": "The authors conducted a systematic psychodynamic investigation directed explicitly toward the bereavement reactions of people who have lost a parent during their adult life. They conducted a single in-depth interview of 14 people with a mean age of 32.4 years who had experienced the death of a parent in the last 3--20 months. The authors present clinical data illustrating several themes, including the importance of the last meeting with the parent, the experience of grief and aggression, coping resources, and growth-oriented aspects of the bereavement process. They also make a number of suggestions regarding clinical practice and further research.", "contents": "Adults' reactions to the death of a parent: a preliminary study. The authors conducted a systematic psychodynamic investigation directed explicitly toward the bereavement reactions of people who have lost a parent during their adult life. They conducted a single in-depth interview of 14 people with a mean age of 32.4 years who had experienced the death of a parent in the last 3--20 months. The authors present clinical data illustrating several themes, including the importance of the last meeting with the parent, the experience of grief and aggression, coping resources, and growth-oriented aspects of the bereavement process. They also make a number of suggestions regarding clinical practice and further research."}
{"id": "PMID:474803", "title": "Developing a curriculum in psychogeriatrics.", "content": "Psychiatric problems are rampant among the aged, yet the psychiatric profession has not developed sufficient resources for training the necessary number of practitioners able and willing to treat elderly psychiatric patients. The management of psychiatric problems in old age differs substantially from that in younger age groups. The elderly patient is likely to have multiple needs and to require diverse services. Fundamental goals in training geriatric psychiatrists should focus on differential diagnosis and treatment, pharmacologic issues, consultation, community resources, and psychiatric, medical, and psychosocial aspects of care. The authors describe a curriculum tailored to meet these goals.", "contents": "Developing a curriculum in psychogeriatrics. Psychiatric problems are rampant among the aged, yet the psychiatric profession has not developed sufficient resources for training the necessary number of practitioners able and willing to treat elderly psychiatric patients. The management of psychiatric problems in old age differs substantially from that in younger age groups. The elderly patient is likely to have multiple needs and to require diverse services. Fundamental goals in training geriatric psychiatrists should focus on differential diagnosis and treatment, pharmacologic issues, consultation, community resources, and psychiatric, medical, and psychosocial aspects of care. The authors describe a curriculum tailored to meet these goals."}
{"id": "PMID:474804", "title": "Models of a heroin epidemic.", "content": "Heroin addiction may be considered an epidemic disease that is communicated by people who are addicted to the drug. It has been suggested that the most recent epidemic in the United States had its peak incidence in 1969. The age of heroin addicts entering treatment has increased systematically from 1973 to 1976 at a rate of less than one year of age per calendar year. This pattern is consistent with a moderate decline in a national heroin epidemic or a geographical migration of the epidemic from more to less populated areas. There are also seasonal trends in the age of admission to treatment.", "contents": "Models of a heroin epidemic. Heroin addiction may be considered an epidemic disease that is communicated by people who are addicted to the drug. It has been suggested that the most recent epidemic in the United States had its peak incidence in 1969. The age of heroin addicts entering treatment has increased systematically from 1973 to 1976 at a rate of less than one year of age per calendar year. This pattern is consistent with a moderate decline in a national heroin epidemic or a geographical migration of the epidemic from more to less populated areas. There are also seasonal trends in the age of admission to treatment."}
{"id": "PMID:474806", "title": "A new method for treating \"headaches\".", "content": "The author describes a 10-week study designed to gain some understanding of how to treat patients who have suffered for a long time with headaches that have not been alleviated by many attempts at treatment. Fourteen patients whose chief complaint was this kind of headache were given varying amounts of perphanazine and amitriptyline with the direct suggestion that these drugs would be helpful. This treatment appeared successful for 10 of the 14 patients at 6-month and 3-year follow-up.", "contents": "A new method for treating \"headaches\". The author describes a 10-week study designed to gain some understanding of how to treat patients who have suffered for a long time with headaches that have not been alleviated by many attempts at treatment. Fourteen patients whose chief complaint was this kind of headache were given varying amounts of perphanazine and amitriptyline with the direct suggestion that these drugs would be helpful. This treatment appeared successful for 10 of the 14 patients at 6-month and 3-year follow-up."}
{"id": "PMID:474807", "title": "Opiate addiction and suicidal behaviors.", "content": "The authors tested the hypothesis that people addicted to opiates manifest more psychopathology in areas that have been identified as predictors for high risk of suicidal behavior than do normal control subjects. They gave 278 patients in a methadone maintenance program and 207 normal control subjects the index of Potential Suicide (IPS), a scale designed to assess suicidal risk. Using discriminate function analysis, the authors found that 87% of 100 of the methadone patients and 98% of 100 of the normal control subjects were correctly identified on the basis of the IPS data.", "contents": "Opiate addiction and suicidal behaviors. The authors tested the hypothesis that people addicted to opiates manifest more psychopathology in areas that have been identified as predictors for high risk of suicidal behavior than do normal control subjects. They gave 278 patients in a methadone maintenance program and 207 normal control subjects the index of Potential Suicide (IPS), a scale designed to assess suicidal risk. Using discriminate function analysis, the authors found that 87% of 100 of the methadone patients and 98% of 100 of the normal control subjects were correctly identified on the basis of the IPS data."}
{"id": "PMID:474808", "title": "Implicit preadmission screening criteria in an alcoholism treatment program.", "content": "The fact that alcoholism programs are biased in terms of the kind of patients admitted to treatment is well recognized. The authors examined the possible bias of preadmission screening procedures in an alcoholism treatment program and investigated some of the criteria used by staff clinicians in determining an applicant's acceptability for treatment. They found that accepted and rejected applicants had highly similar characteristics, differing only in age (accepted applicants were slightly younger than those rejected) and source of referral. The overall findings suggest that clinicians implicitly evaluate problem drinkers along dimensions related to their perceived \"treatability\" in a given therapeutic setting.", "contents": "Implicit preadmission screening criteria in an alcoholism treatment program. The fact that alcoholism programs are biased in terms of the kind of patients admitted to treatment is well recognized. The authors examined the possible bias of preadmission screening procedures in an alcoholism treatment program and investigated some of the criteria used by staff clinicians in determining an applicant's acceptability for treatment. They found that accepted and rejected applicants had highly similar characteristics, differing only in age (accepted applicants were slightly younger than those rejected) and source of referral. The overall findings suggest that clinicians implicitly evaluate problem drinkers along dimensions related to their perceived \"treatability\" in a given therapeutic setting."}
{"id": "PMID:474809", "title": "Juvenile male sexual assaulters.", "content": "This study compares the psychiatric, neurological, and psychoeducational status of sexually assaultive male juveniles and other violent juveniles. The authors found that juvenile sexual assaulters suffered from neuropsychiatric problems similar to those of other violent juveniles, had committed violent acts other than sexual assault, and had had seriously aberrant behavior since early childhood. The findings contradict prevailing assumptions that sexual assaults by juveniles are rare occurrences and that juvenile sex offenders have low rates of recidivism. Theoretical and treatment implications are discussed.", "contents": "Juvenile male sexual assaulters. This study compares the psychiatric, neurological, and psychoeducational status of sexually assaultive male juveniles and other violent juveniles. The authors found that juvenile sexual assaulters suffered from neuropsychiatric problems similar to those of other violent juveniles, had committed violent acts other than sexual assault, and had had seriously aberrant behavior since early childhood. The findings contradict prevailing assumptions that sexual assaults by juveniles are rare occurrences and that juvenile sex offenders have low rates of recidivism. Theoretical and treatment implications are discussed."}
{"id": "PMID:474833", "title": "Hypnotic deafness: a psychophysical study of responses to tone intensity as modified by hypnosis.", "content": "Hypnotic deafness was suggested for 1000 Hz tones presented in random orders at seven intensities between 17 and 70 db. Subjects were 70 college students stratified into four levels of hypnotic susceptibility, ranging from low to high. Four conditions were presented within a single session. Two conditions tested normal hearing, one in waking and one in hypnosis; two tested reported loudness of the tones as reduced by hypnotic suggestion. The method of magnitude estimation was employed. Hearing reduction was found to correlate .59 with hypnotic susceptibility in the total sample. Few high hypnotizables reduced their hearing to zero; their mean residual hearing during the deafness conditions was 55% of normal. Power functions for the relationship between tone intensity and magnitude estimates for conditions of normal hearing and deafness were found to be relatively parallel and orderly, differing primarily in intercept value. Order effect anomalies are discussed. The \"hidden observer\" method showed that for 4 of the 70 subjects the covert hearing was found to be at least 20% greater than that reported overtly within hypnotic deafness and approached normal hearing. As in our previous hypnotic analgesia research, not all subjects who reduced their hearing significantly gave subsequent covert reports which differed from reported overt hearing. Discussion is given for evidence of two levels of information processing during hypnotically suggested perceptual distortions.", "contents": "Hypnotic deafness: a psychophysical study of responses to tone intensity as modified by hypnosis. Hypnotic deafness was suggested for 1000 Hz tones presented in random orders at seven intensities between 17 and 70 db. Subjects were 70 college students stratified into four levels of hypnotic susceptibility, ranging from low to high. Four conditions were presented within a single session. Two conditions tested normal hearing, one in waking and one in hypnosis; two tested reported loudness of the tones as reduced by hypnotic suggestion. The method of magnitude estimation was employed. Hearing reduction was found to correlate .59 with hypnotic susceptibility in the total sample. Few high hypnotizables reduced their hearing to zero; their mean residual hearing during the deafness conditions was 55% of normal. Power functions for the relationship between tone intensity and magnitude estimates for conditions of normal hearing and deafness were found to be relatively parallel and orderly, differing primarily in intercept value. Order effect anomalies are discussed. The \"hidden observer\" method showed that for 4 of the 70 subjects the covert hearing was found to be at least 20% greater than that reported overtly within hypnotic deafness and approached normal hearing. As in our previous hypnotic analgesia research, not all subjects who reduced their hearing significantly gave subsequent covert reports which differed from reported overt hearing. Discussion is given for evidence of two levels of information processing during hypnotically suggested perceptual distortions."}
{"id": "PMID:474834", "title": "Cognitive processes in the solution of three-term series problems.", "content": "This study investigated the nature of strategies used in solving the three-term series problem. Three presentation modes (auditory, visual/sequential, and visual/simultaneous) were crossed with two question positions (before-premises and after-premises), for a total of six methods of problem presentation. Both high-spatial/imagal and low-spatial/imagal problems were employed, the assumption being that better performance on high-spatial/imagal problems reflected the use of a spatial/imagal strategy, while equal performance on both types of problems indicated the use of an alternative, perhaps verbal, strategy. It was hypothesized that different presentations would lead to differences in memory demands, input/processing interference, and mathemagenic behaviors, and thus to different problem-solving strategies. Response data and subjective reports confirmed this prediction. Results were discussed in terms of the Clark-Huttenlocher controversy (H. H. Clark, Linguistic processes in deductive reasoning, in Psychological Review, 1969, 76, 387--404; J. Huttenlocher, Constructing spatial images: A strategy in reasoning, Psychological Review, 1968, 75, 550--560).", "contents": "Cognitive processes in the solution of three-term series problems. This study investigated the nature of strategies used in solving the three-term series problem. Three presentation modes (auditory, visual/sequential, and visual/simultaneous) were crossed with two question positions (before-premises and after-premises), for a total of six methods of problem presentation. Both high-spatial/imagal and low-spatial/imagal problems were employed, the assumption being that better performance on high-spatial/imagal problems reflected the use of a spatial/imagal strategy, while equal performance on both types of problems indicated the use of an alternative, perhaps verbal, strategy. It was hypothesized that different presentations would lead to differences in memory demands, input/processing interference, and mathemagenic behaviors, and thus to different problem-solving strategies. Response data and subjective reports confirmed this prediction. Results were discussed in terms of the Clark-Huttenlocher controversy (H. H. Clark, Linguistic processes in deductive reasoning, in Psychological Review, 1969, 76, 387--404; J. Huttenlocher, Constructing spatial images: A strategy in reasoning, Psychological Review, 1968, 75, 550--560)."}
{"id": "PMID:474835", "title": "Stimulus and procedural determinants of the forgetting of conditioned fear.", "content": "Two studies investigated factors believed to influence the retention of acquired fear in rats. The results show clearly that the lack of forgetting over 24-hr interval reported by previous investigators is due to a depression of performance on the immediate test (3-min retention interval) resulting from the procedure of not handling the rats during conditioning but then handling them on every trial during the retention test. In accord with the McAllister's interpretation of related results, the depression of performance after short retention intervals may be due to a lack of generalization from training to the testing situation. As the retention interval is lengthened, the amount of generalization increases and escape performance improves on a relative basis. The nature of the retention function may be severely distorted by selection of an inappropriate paradigm for its evaluation.", "contents": "Stimulus and procedural determinants of the forgetting of conditioned fear. Two studies investigated factors believed to influence the retention of acquired fear in rats. The results show clearly that the lack of forgetting over 24-hr interval reported by previous investigators is due to a depression of performance on the immediate test (3-min retention interval) resulting from the procedure of not handling the rats during conditioning but then handling them on every trial during the retention test. In accord with the McAllister's interpretation of related results, the depression of performance after short retention intervals may be due to a lack of generalization from training to the testing situation. As the retention interval is lengthened, the amount of generalization increases and escape performance improves on a relative basis. The nature of the retention function may be severely distorted by selection of an inappropriate paradigm for its evaluation."}
{"id": "PMID:474836", "title": "The repetition effect in judgments of temporal duration across minutes, days, and months.", "content": "Three experiments assessed the stability of the repetition effect in time judgment. In Experiment 1, subjects (N = 18) produced intervals ranging from 8 to 31 sec in a two-phase procedure with a five min rest between phases. In Experiment 2, subjects (N = 20) made verbal estimates of intervals ranging from 8 to 20 sec with a 48-hour delay between phases. Experiment 3 involved daily productions by three subjects of intervals ranging from 8 to 54 sec over several months. The slope of the psychophysical function increased across phases in Experimental 1, declined (as expected for estimation) across days in Experiment 2, and increased across months in Experiment 3. It was concluded that the repetition effect is relatively permanent and thus more similar to habituation than to sensory adaptation.", "contents": "The repetition effect in judgments of temporal duration across minutes, days, and months. Three experiments assessed the stability of the repetition effect in time judgment. In Experiment 1, subjects (N = 18) produced intervals ranging from 8 to 31 sec in a two-phase procedure with a five min rest between phases. In Experiment 2, subjects (N = 20) made verbal estimates of intervals ranging from 8 to 20 sec with a 48-hour delay between phases. Experiment 3 involved daily productions by three subjects of intervals ranging from 8 to 54 sec over several months. The slope of the psychophysical function increased across phases in Experimental 1, declined (as expected for estimation) across days in Experiment 2, and increased across months in Experiment 3. It was concluded that the repetition effect is relatively permanent and thus more similar to habituation than to sensory adaptation."}
{"id": "PMID:474837", "title": "Integration of verbal and visual information as evidenced by distortions in picture memory.", "content": "Subjects were presented with a series of pictures, some of which were general (girl walking down the path) and others specific (girl hiking down the path). These pictures were matched with sentences which were either general or specific (\"The girl is walking [hiking] down the path.\") Subsequently, a forced-choice picture recognition test was administered in which subjects saw pairs of pictures and indicated which member of each pair they had seen before. It was found that labelling the picture with a sentence containing a specific verb substantially increased the likelihood that the specific picture corresponding to that verb would subsequently be falsely recognized. The results are discussed in terms of current theories of memorial representation.", "contents": "Integration of verbal and visual information as evidenced by distortions in picture memory. Subjects were presented with a series of pictures, some of which were general (girl walking down the path) and others specific (girl hiking down the path). These pictures were matched with sentences which were either general or specific (\"The girl is walking [hiking] down the path.\") Subsequently, a forced-choice picture recognition test was administered in which subjects saw pairs of pictures and indicated which member of each pair they had seen before. It was found that labelling the picture with a sentence containing a specific verb substantially increased the likelihood that the specific picture corresponding to that verb would subsequently be falsely recognized. The results are discussed in terms of current theories of memorial representation."}
{"id": "PMID:474841", "title": "Causes of fetal and neonatal mortality by race in a selected U.S. population.", "content": "In a large prospective study (1959--1966), the perinatal mortality rate for U.S. Whites was 34 deaths per 1000 total births, for Blacks 51/1000, for Puerto Ricans 41/1000, and for Orientals 23/1000. A number of disorders were responsible for these differences. Premature rupture of the fetal membranes occurred 92 per cent more often in Blacks than in Whites, marked placental growth retardation 56 per cent more often, amniotic fluid infections 45 per cent more often, and major congenital malformations 15 per cent more often. Most other disorders were less common in Blacks than in Whites. Stratifying the data by selected factors, such as prepregnancy body weight and antenatal medical care, eliminated or greatly reduced almost all of these interracial differences. The only major unexplained differences remaining were an excess of amniotic fluid infections and major congenital malformations in Blacks, and an excess of abruptio placentae and large placental infarcts in Whites.", "contents": "Causes of fetal and neonatal mortality by race in a selected U.S. population. In a large prospective study (1959--1966), the perinatal mortality rate for U.S. Whites was 34 deaths per 1000 total births, for Blacks 51/1000, for Puerto Ricans 41/1000, and for Orientals 23/1000. A number of disorders were responsible for these differences. Premature rupture of the fetal membranes occurred 92 per cent more often in Blacks than in Whites, marked placental growth retardation 56 per cent more often, amniotic fluid infections 45 per cent more often, and major congenital malformations 15 per cent more often. Most other disorders were less common in Blacks than in Whites. Stratifying the data by selected factors, such as prepregnancy body weight and antenatal medical care, eliminated or greatly reduced almost all of these interracial differences. The only major unexplained differences remaining were an excess of amniotic fluid infections and major congenital malformations in Blacks, and an excess of abruptio placentae and large placental infarcts in Whites."}
{"id": "PMID:474842", "title": "Cesarean section, fetal monitoring, and perinatal mortality in California.", "content": "The rate of cesarean section in California has been growing at a compound rate of about 10 per cent per annum since 1969, coinciding with the advent of fetal monitoring. It is of interest, therefore, to study the distribution and efficacy of obstetric interventions. Information derived from the 1977 California birth cohort and a survey questionnaire was used to study the factors associated with the rate of cesarean section in 323 hospitals. Significant positive correlations were observed between the cesarean rate (CSR) and hospital factors indicative of a high degree of technology, including the proportion of labors electronically monitored. Significant negative correlations were observed between the CSR and hospital-specific variables suggestive of socioeconomically underprivileged patient populations. Other factors being constant, hospitals characterized by prepayment health care financing also had lower CSRs. A standaridized mortality ratio (SMR) based on 2.3 million births in the 1970--1976 cohorts was used to adjust the 1977 hospital specific perinatal mortality rates for birth weight, gestational age, sex, race, and plurality. The results show that hospitals which intervene technologically in a large proportion of births have lower risk adjusted perinatal mortality rates.", "contents": "Cesarean section, fetal monitoring, and perinatal mortality in California. The rate of cesarean section in California has been growing at a compound rate of about 10 per cent per annum since 1969, coinciding with the advent of fetal monitoring. It is of interest, therefore, to study the distribution and efficacy of obstetric interventions. Information derived from the 1977 California birth cohort and a survey questionnaire was used to study the factors associated with the rate of cesarean section in 323 hospitals. Significant positive correlations were observed between the cesarean rate (CSR) and hospital factors indicative of a high degree of technology, including the proportion of labors electronically monitored. Significant negative correlations were observed between the CSR and hospital-specific variables suggestive of socioeconomically underprivileged patient populations. Other factors being constant, hospitals characterized by prepayment health care financing also had lower CSRs. A standaridized mortality ratio (SMR) based on 2.3 million births in the 1970--1976 cohorts was used to adjust the 1977 hospital specific perinatal mortality rates for birth weight, gestational age, sex, race, and plurality. The results show that hospitals which intervene technologically in a large proportion of births have lower risk adjusted perinatal mortality rates."}
{"id": "PMID:474843", "title": "An evaluation of efforts to educate mothers about child development in pediatric office practices.", "content": "The efforts expended by pediatricians in a variety of private practice settings to educate mothers of first-born children about child behavior and development were examined in relation to various outcome measures of mother and child functioning over a time period of one-and-a-half years. Mothers learned more about child development in group settings than in solo practice settings, but differences between medical groups with and without nurse practitioners were not significant. Mothers receiving care from pediatricians who made at least a moderate effort to teach, learned more about child development, described more use of positive contact with their children, and felt they were helped more in their childrearing efforts than did mothers receiving care from pediatricians who made little effort to teach. However, there were no significant differences in measures of the child's developmental status related to physician teaching input, and mothers exposed to higher levels of teaching input reported more behavioral problems with their children. The most important predictor of the child's developmental status at 18 months of age was the amount of positive contact between mother and child at one year. It is suggested that the effects of changing the frequency of well-child visits on the mothers' interaction patterns with their children and on their feelings of being supported by the physician be ascertained before making recommendations about the optimal number of such visits.", "contents": "An evaluation of efforts to educate mothers about child development in pediatric office practices. The efforts expended by pediatricians in a variety of private practice settings to educate mothers of first-born children about child behavior and development were examined in relation to various outcome measures of mother and child functioning over a time period of one-and-a-half years. Mothers learned more about child development in group settings than in solo practice settings, but differences between medical groups with and without nurse practitioners were not significant. Mothers receiving care from pediatricians who made at least a moderate effort to teach, learned more about child development, described more use of positive contact with their children, and felt they were helped more in their childrearing efforts than did mothers receiving care from pediatricians who made little effort to teach. However, there were no significant differences in measures of the child's developmental status related to physician teaching input, and mothers exposed to higher levels of teaching input reported more behavioral problems with their children. The most important predictor of the child's developmental status at 18 months of age was the amount of positive contact between mother and child at one year. It is suggested that the effects of changing the frequency of well-child visits on the mothers' interaction patterns with their children and on their feelings of being supported by the physician be ascertained before making recommendations about the optimal number of such visits."}
{"id": "PMID:474844", "title": "The risk of low birthweight.", "content": "Descriptive analyses of birthweight for single live births in the United States during 1974, using birth certificate information, show that several factors are associated with a high incidence of low birthweight babies. Multivariate analyses were performed to determine correlates of low birthweight. When other factors are held constant, race not white, previous reproductive loss, short interpregnancy interval, out-of-wedlock birth, no prenatal care, and maternal age under 18 years or over 35 years each increase the risk of having an infant of low birthweight. High birth order and maternal education under 12 years of schooling were not consistent risk factors for low birthweight. Odds ratios are presented and the method for combining these, to estimate the risk for an individual mother having a low-birthweight infant, is illustrated.", "contents": "The risk of low birthweight. Descriptive analyses of birthweight for single live births in the United States during 1974, using birth certificate information, show that several factors are associated with a high incidence of low birthweight babies. Multivariate analyses were performed to determine correlates of low birthweight. When other factors are held constant, race not white, previous reproductive loss, short interpregnancy interval, out-of-wedlock birth, no prenatal care, and maternal age under 18 years or over 35 years each increase the risk of having an infant of low birthweight. High birth order and maternal education under 12 years of schooling were not consistent risk factors for low birthweight. Odds ratios are presented and the method for combining these, to estimate the risk for an individual mother having a low-birthweight infant, is illustrated."}
{"id": "PMID:474845", "title": "Treatment of women in childbirth: implications for family beginnings.", "content": "This paper presents an ethnographic analysis of a woman's interaction with staff members during the early stage of labor, focusing on the factors that shape the childbirth experience. The observational data were gathered in several hospitals as part of a study of the medical context of childbearing. The paper presents a model of naturalistic inquiry into the transition to parenthood. Nurses and physicians are found to offer arbitrary, uniform, and often inappropriate responses to birthing women that may inhibit well-being.", "contents": "Treatment of women in childbirth: implications for family beginnings. This paper presents an ethnographic analysis of a woman's interaction with staff members during the early stage of labor, focusing on the factors that shape the childbirth experience. The observational data were gathered in several hospitals as part of a study of the medical context of childbearing. The paper presents a model of naturalistic inquiry into the transition to parenthood. Nurses and physicians are found to offer arbitrary, uniform, and often inappropriate responses to birthing women that may inhibit well-being."}
{"id": "PMID:474846", "title": "A comparison of growth: Spanish-surnamed with non-Spanish-surnamed children.", "content": "Weight, height, and head circumference measurements of 4,167 Spanish-surnamed school-aged children were compared with similar data from 2,322 non-Spanish surnamed children who resided in the same Denver, Colorado neighborhoods. These data were also compared with data from six other studies. Both male and female Spanish-surnamed children were found to weigh less, be shorter, and have smaller head circumferences than non-Spanish-surnamed children living in the same Denver neighborhoods. The sizes of the children in these two populations residing in lower and lower-middle class neighborhoods were closer to each other than to the sizes of children from middle and upper-middle socioeconomic classes as measured in previous studies or to the sizes of children in the recently published cross-sectional National Center for Health Statistics study. Such comparisons suggest that growth retardation is more a reflection of socioeconomic factors than of ethnic-genetic factors.", "contents": "A comparison of growth: Spanish-surnamed with non-Spanish-surnamed children. Weight, height, and head circumference measurements of 4,167 Spanish-surnamed school-aged children were compared with similar data from 2,322 non-Spanish surnamed children who resided in the same Denver, Colorado neighborhoods. These data were also compared with data from six other studies. Both male and female Spanish-surnamed children were found to weigh less, be shorter, and have smaller head circumferences than non-Spanish-surnamed children living in the same Denver neighborhoods. The sizes of the children in these two populations residing in lower and lower-middle class neighborhoods were closer to each other than to the sizes of children from middle and upper-middle socioeconomic classes as measured in previous studies or to the sizes of children in the recently published cross-sectional National Center for Health Statistics study. Such comparisons suggest that growth retardation is more a reflection of socioeconomic factors than of ethnic-genetic factors."}
{"id": "PMID:474847", "title": "Attitudes of mothers toward sex education.", "content": "Data are presented on the attitudes of mothers from the entire social class spectrum toward content and timing of sex education for children (CT-Attitudes), and also toward sex education in school (S-Attitudes) in two California communities in 1969. Findings underscore the necessity to consider these two attitudinal variables separately. As a result of their separation for study purposes, it was possible to arrive at a four-fold typology or grouping of mothers: 1) CT liberals in favor, and 2) CT liberals opposed to sex education in schools, 3) CT conservatives in favor, and 4) CT conservatives opposed to school programs of sex education. It is inferred that educational planners need to pay due regard to the sentiments of all four maternal groups and all social classes in developing sex education programs for families and for school children.", "contents": "Attitudes of mothers toward sex education. Data are presented on the attitudes of mothers from the entire social class spectrum toward content and timing of sex education for children (CT-Attitudes), and also toward sex education in school (S-Attitudes) in two California communities in 1969. Findings underscore the necessity to consider these two attitudinal variables separately. As a result of their separation for study purposes, it was possible to arrive at a four-fold typology or grouping of mothers: 1) CT liberals in favor, and 2) CT liberals opposed to sex education in schools, 3) CT conservatives in favor, and 4) CT conservatives opposed to school programs of sex education. It is inferred that educational planners need to pay due regard to the sentiments of all four maternal groups and all social classes in developing sex education programs for families and for school children."}
{"id": "PMID:474848", "title": "Evaluation of the relationship of sodium in drinking water and toxemia of pregnancy.", "content": "This study is a retrospective case comparison analysis of the relationship of water-borne Na+ and toxemia of pregnancy as a part of a continuous investigation at our facility on the role of various water constituents in human chronic disease. Five hundred thirty-seven toxemic pregnant women delivered at Jefferson Davis Hospital, Houston, Texas, during 1976 were matched by age, race, obstetrical history, and month of delivery to 537 non-toxemic controls. Sodium concentration in each patient's drinking water was derived, based on her address, from earlier prepared maps of sodium distribution in Houston's water supplies. Paired t-tests were performed to compare the Na+ levels, which varied from 19 to 250 mg/l in water supplies of cases and controls. Odds ratio analysis was used to test for the presence of a gradient in occurrence of toxemia in parallel with the gradient of Na+. No significant impact of water-borne Na+ in the indicated range was observed. Further studies incorporating still higher ranges are contemplated.", "contents": "Evaluation of the relationship of sodium in drinking water and toxemia of pregnancy. This study is a retrospective case comparison analysis of the relationship of water-borne Na+ and toxemia of pregnancy as a part of a continuous investigation at our facility on the role of various water constituents in human chronic disease. Five hundred thirty-seven toxemic pregnant women delivered at Jefferson Davis Hospital, Houston, Texas, during 1976 were matched by age, race, obstetrical history, and month of delivery to 537 non-toxemic controls. Sodium concentration in each patient's drinking water was derived, based on her address, from earlier prepared maps of sodium distribution in Houston's water supplies. Paired t-tests were performed to compare the Na+ levels, which varied from 19 to 250 mg/l in water supplies of cases and controls. Odds ratio analysis was used to test for the presence of a gradient in occurrence of toxemia in parallel with the gradient of Na+. No significant impact of water-borne Na+ in the indicated range was observed. Further studies incorporating still higher ranges are contemplated."}
{"id": "PMID:474849", "title": "Measles in a partially immunized community.", "content": "During the investigation of a measles outbreak in a rural community, a spectrum of illness was observed which ranged in severity from a brief febrile illness to one characteristic of typical measles. Unimmunized children were more apt to develop measles (p less than 0.001) than were immunized children. However, a proportion of both the immunized and unimmunized children developed a milder, \"measles-associated,\" illness. Employing a logistic model, it was demonstrated that the probability of developing either measles or \"measles-associated\" illness decreased with increasing age at immunization but was not affected by the interval since immunization.", "contents": "Measles in a partially immunized community. During the investigation of a measles outbreak in a rural community, a spectrum of illness was observed which ranged in severity from a brief febrile illness to one characteristic of typical measles. Unimmunized children were more apt to develop measles (p less than 0.001) than were immunized children. However, a proportion of both the immunized and unimmunized children developed a milder, \"measles-associated,\" illness. Employing a logistic model, it was demonstrated that the probability of developing either measles or \"measles-associated\" illness decreased with increasing age at immunization but was not affected by the interval since immunization."}
{"id": "PMID:474850", "title": "Policies toward medical technology: the case of electronic fetal monitoring.", "content": "Electronic fetal monitoring (EFM) is an example of a medical technology that has been widely accepted since its introduction in the mid-1960s. However, review of the literature does not provide convincing evidence of EFM efficacy, and four recent, controlled, clinical trails show little if any benefit in terms of preventing death or long-term disability of the baby. Public and private policies have largely acted to encourage use of EFM, and none have acted to slow or prevent its spread. This need for mechanisms to assure the timely evaluation of new medical technologies before they are accepted as a medical practice has led to a new medical devices program in the Food and Drug Administration, consensus development groups at the National Institutes of Health, and congressional legislation to establish a new National Center for Health Care Technology.", "contents": "Policies toward medical technology: the case of electronic fetal monitoring. Electronic fetal monitoring (EFM) is an example of a medical technology that has been widely accepted since its introduction in the mid-1960s. However, review of the literature does not provide convincing evidence of EFM efficacy, and four recent, controlled, clinical trails show little if any benefit in terms of preventing death or long-term disability of the baby. Public and private policies have largely acted to encourage use of EFM, and none have acted to slow or prevent its spread. This need for mechanisms to assure the timely evaluation of new medical technologies before they are accepted as a medical practice has led to a new medical devices program in the Food and Drug Administration, consensus development groups at the National Institutes of Health, and congressional legislation to establish a new National Center for Health Care Technology."}
{"id": "PMID:474851", "title": "Validation of a poison prevention program.", "content": "The effectiveness of an empirically designed poison warning label and an educational program for three and four year old children was assessed in four groups of 39 such children, with one group serving as a control. The educational program appeared to improve intellectual (verbal) awareness of poisons, the label to improve visual discrimination, and the combination of the two to have the greatest overall impact.", "contents": "Validation of a poison prevention program. The effectiveness of an empirically designed poison warning label and an educational program for three and four year old children was assessed in four groups of 39 such children, with one group serving as a control. The educational program appeared to improve intellectual (verbal) awareness of poisons, the label to improve visual discrimination, and the combination of the two to have the greatest overall impact."}
{"id": "PMID:474852", "title": "Response of low income women and abortion facilities to restriction of public funds for abortion: a study of a large metropolitan area.", "content": "During the first five months after the restriction of public funds for abortion, a large percentage of low-income women seeking abortions in a metropolitan area in Texas obtained abortion that were partially subsizied using a combination of reduced clinic fees and public funds for ancillary non-abortion services. Their own personal funds made up the difference between the subsidy and the full cost of the procedure.", "contents": "Response of low income women and abortion facilities to restriction of public funds for abortion: a study of a large metropolitan area. During the first five months after the restriction of public funds for abortion, a large percentage of low-income women seeking abortions in a metropolitan area in Texas obtained abortion that were partially subsizied using a combination of reduced clinic fees and public funds for ancillary non-abortion services. Their own personal funds made up the difference between the subsidy and the full cost of the procedure."}
{"id": "PMID:474859", "title": "Popliteal hiatus of the lateral meniscus. Anatomy and measurement at dissection of 10 specimens.", "content": "The popliteal hiatus is the opening defined by the fascicles of the lateral meniscus, which permits the popliteal tendon to pursue its course from the tibia to its femoral attachment. The medial border of the popliteal hiatus is the body of the meniscus. Ten fresh-frozen knee specimens from human adult males (with no evidence of previous surgery to the knees) were dissected. The joints were disarticulated so that only the lateral structures remained intact. The perimeter of the meniscal attachment anterior to the hiatus measured 4.2+/ - 0.3 cm, and the perimeter posterior to the hiatus measured 3.4 +/- 0.5 cm. The total perimeter of the lateral meniscus was 8.9 +/- 0.7 cm. The length of the superior fascicle of the lateral meniscus was 0.9 +/- 0.2 cm. In this series, the length of the hiatus was 1.3 +/- 0.1 cm. The anatomy of the popliteal hiatus was constant. The popliteal hiatus is an anatomical structure and must be differentiated from pathologic lesions common to the same area.", "contents": "Popliteal hiatus of the lateral meniscus. Anatomy and measurement at dissection of 10 specimens. The popliteal hiatus is the opening defined by the fascicles of the lateral meniscus, which permits the popliteal tendon to pursue its course from the tibia to its femoral attachment. The medial border of the popliteal hiatus is the body of the meniscus. Ten fresh-frozen knee specimens from human adult males (with no evidence of previous surgery to the knees) were dissected. The joints were disarticulated so that only the lateral structures remained intact. The perimeter of the meniscal attachment anterior to the hiatus measured 4.2+/ - 0.3 cm, and the perimeter posterior to the hiatus measured 3.4 +/- 0.5 cm. The total perimeter of the lateral meniscus was 8.9 +/- 0.7 cm. The length of the superior fascicle of the lateral meniscus was 0.9 +/- 0.2 cm. In this series, the length of the hiatus was 1.3 +/- 0.1 cm. The anatomy of the popliteal hiatus was constant. The popliteal hiatus is an anatomical structure and must be differentiated from pathologic lesions common to the same area."}
{"id": "PMID:474863", "title": "Bladder trauma in the long-distance runner.", "content": "The occurrence of hematuria as a short-lived and occasional event in a number of long-distance runners appears to be secondary to bladder contusions caused by a combination of exertional forces and intraabdominal pressure producing repeated impact of the flaccid wall of the bladder against the bladder base. The bladder may be empty or nearly empty while the runner is in action. This permits apposition of the surfaces of the bladder. The variability of the state of filling of one's bladder from one occasion to another may therefore account for the inconsistency of occurrence of hematuria in the same runner.", "contents": "Bladder trauma in the long-distance runner. The occurrence of hematuria as a short-lived and occasional event in a number of long-distance runners appears to be secondary to bladder contusions caused by a combination of exertional forces and intraabdominal pressure producing repeated impact of the flaccid wall of the bladder against the bladder base. The bladder may be empty or nearly empty while the runner is in action. This permits apposition of the surfaces of the bladder. The variability of the state of filling of one's bladder from one occasion to another may therefore account for the inconsistency of occurrence of hematuria in the same runner."}
{"id": "PMID:474862", "title": "An epidemiologic study of tennis elbow. Incidence, recurrence, and effectiveness of prevention strategies.", "content": "An epidemiologic study of the incidence and recurrence of tennis elbow among over 500 tennis players (278 men, 254 women; age range, 20 to 50 years) indicated that age and amount of playing time per day were contributing factors to the injury. Both incidence and recurrence rates increased with age. An interactive effect of playing time and age was observed with increased playing time associated with higher incidence at younger ages. Larger grip size was also associated with higher incidence in the older group. These findings were interpreted as being consistent with the hypothesis that tennis elbow is a degenerative disease, the onset of which is hastened by overuse of the arm and elbow. Changes in stroke technique and types of racket were successful in preventing recurrence. Least successful was the forearm brace.", "contents": "An epidemiologic study of tennis elbow. Incidence, recurrence, and effectiveness of prevention strategies. An epidemiologic study of the incidence and recurrence of tennis elbow among over 500 tennis players (278 men, 254 women; age range, 20 to 50 years) indicated that age and amount of playing time per day were contributing factors to the injury. Both incidence and recurrence rates increased with age. An interactive effect of playing time and age was observed with increased playing time associated with higher incidence at younger ages. Larger grip size was also associated with higher incidence in the older group. These findings were interpreted as being consistent with the hypothesis that tennis elbow is a degenerative disease, the onset of which is hastened by overuse of the arm and elbow. Changes in stroke technique and types of racket were successful in preventing recurrence. Least successful was the forearm brace."}
{"id": "PMID:474864", "title": "Injuries in women's gymnastics. A 5-year study.", "content": "In this 5-year study of women gymnasts, 70 participants (i.e., one woman for one season) sustained 66 major injuries (i.e., brought to the attention of a physician and produced disability). Forty-seven of the 70 athletes sustained injuries. The trunk had the lowest incidence of injuries (11), the upper extremity had 20, and the lower extremity had the highest incidence (35 injuries). Two categories, stress or trauma, segregated the injuries and provided rationale for the high incidence of injuries on this team. These athletes were of national caliber. They were constantly trying to improve performance. They engaged in intensive practice and difficult maneuvers. The self-motivation of these women increased the number of injuries caused by stress (a total of 21); the combination of intensive practice and the maneuvers is responsible for the traumatic injuries (45). From this evidence, women's gymnastics should be recognized as a hazardous sport for the competitor. Risk factors should be evaluated constantly by her, the coach, and the team physician.", "contents": "Injuries in women's gymnastics. A 5-year study. In this 5-year study of women gymnasts, 70 participants (i.e., one woman for one season) sustained 66 major injuries (i.e., brought to the attention of a physician and produced disability). Forty-seven of the 70 athletes sustained injuries. The trunk had the lowest incidence of injuries (11), the upper extremity had 20, and the lower extremity had the highest incidence (35 injuries). Two categories, stress or trauma, segregated the injuries and provided rationale for the high incidence of injuries on this team. These athletes were of national caliber. They were constantly trying to improve performance. They engaged in intensive practice and difficult maneuvers. The self-motivation of these women increased the number of injuries caused by stress (a total of 21); the combination of intensive practice and the maneuvers is responsible for the traumatic injuries (45). From this evidence, women's gymnastics should be recognized as a hazardous sport for the competitor. Risk factors should be evaluated constantly by her, the coach, and the team physician."}
{"id": "PMID:474860", "title": "The saphenous nerve: its course and importance in medial arthrotomy.", "content": "Both lower extremities of 10 cadavers (20 specimens) were dissected to delineate the course of the saphenous nerve and its two major divisions (sartorial and infrapatellar branches). The course of the saphenous nerve followed the standard text description, except at the point in the sartorius muscle where the infrapatellar branch exited to become a subcutaneous structure. The location of this branch varied slightly in each cadaver but was the same for both lower extremities in the same cadaver. The location of the sartorial nerve and its relationship to the tendons of the pes anserinus was consistant in all 20 specimens. Since 69% of a group of 75 patients found altered sensation significant after routine sectioning of the infrapatellar nerve following medial meniscectomy, a group of surgeons at the University of Michigan is now protecting the infrapatellar branch of the saphenous nerve at operation. Early results on a small number of patients indicate that no alteration in sensation occurs if the nerve is carefully retracted.", "contents": "The saphenous nerve: its course and importance in medial arthrotomy. Both lower extremities of 10 cadavers (20 specimens) were dissected to delineate the course of the saphenous nerve and its two major divisions (sartorial and infrapatellar branches). The course of the saphenous nerve followed the standard text description, except at the point in the sartorius muscle where the infrapatellar branch exited to become a subcutaneous structure. The location of this branch varied slightly in each cadaver but was the same for both lower extremities in the same cadaver. The location of the sartorial nerve and its relationship to the tendons of the pes anserinus was consistant in all 20 specimens. Since 69% of a group of 75 patients found altered sensation significant after routine sectioning of the infrapatellar nerve following medial meniscectomy, a group of surgeons at the University of Michigan is now protecting the infrapatellar branch of the saphenous nerve at operation. Early results on a small number of patients indicate that no alteration in sensation occurs if the nerve is carefully retracted."}
{"id": "PMID:474865", "title": "Injury patterns in children and adolescent skiers.", "content": "Over 3,500 students from five major high schools in the Seattle metropolitan area were studied as to skiing injuries. Each had skiied an average of 13.3 days. The average age of t'he group was 13.5 years, the ages ranging from 3 to 19 years. The students 10 years and under had the lowest incidence of injuries, those aged 11 to 14 years had the highest over-all injury rates, and the group 15 years of age and over laid midway between these two groups in injury rate. There was no evidence in the study that the open physis led to a higher incidence of epiphyseal injuries. The knee was more commonly involved than the ankle, and sprains, contusions, and fractures followed in that order as far as injury rates were concerned. The female was more prone to injury than the male. Skiiers with greater ability had fewer injuries than those with less skill in this particular sport.", "contents": "Injury patterns in children and adolescent skiers. Over 3,500 students from five major high schools in the Seattle metropolitan area were studied as to skiing injuries. Each had skiied an average of 13.3 days. The average age of t'he group was 13.5 years, the ages ranging from 3 to 19 years. The students 10 years and under had the lowest incidence of injuries, those aged 11 to 14 years had the highest over-all injury rates, and the group 15 years of age and over laid midway between these two groups in injury rate. There was no evidence in the study that the open physis led to a higher incidence of epiphyseal injuries. The knee was more commonly involved than the ankle, and sprains, contusions, and fractures followed in that order as far as injury rates were concerned. The female was more prone to injury than the male. Skiiers with greater ability had fewer injuries than those with less skill in this particular sport."}
{"id": "PMID:474861", "title": "Neck motion in the high school football player. Observations and suggestions for diminishing stresses on the neck.", "content": "In a group of 40 high school athletes (height: 166.37 to 189.57 cm, average, 174.40 cm; neck circumference: 35.56 to 41.91 cm, average, 39.12 cm), the neck motions were studied in flexion and extension, clinically, radiographically, and cineoradiographically. Flexion ranged from 34 to 84 degrees (average 72 degrees) and extension from 21 to 64 degrees (average 45 degrees) without helmet and shoulder pads. In well-fitting equipment flexion ranged from 36 to 86 degrees (average 73 degrees) and extension from 12 to 56 degrees(average 34 degrees). No correlation was determined between the size of the athlete's neck and the range of motion. The size of the athlete's neck was important in the determination of maximum stresses in the neck. The helmets impinged on the shoulder pads or interscapular region; this impingement diminished tension on the anterior portion of the cervical spine in extension. The face masks impinged on the shoulder pads in flexion of the neck; this impingement diminished stress on the posterior musculature. Proper fitting equipment, conditioning neck exercises, and changes in the rules of the game so that abuse of the head and neck is not encouraged are some of the aspects that may reduce the risk of injury to the cervical region in football players.", "contents": "Neck motion in the high school football player. Observations and suggestions for diminishing stresses on the neck. In a group of 40 high school athletes (height: 166.37 to 189.57 cm, average, 174.40 cm; neck circumference: 35.56 to 41.91 cm, average, 39.12 cm), the neck motions were studied in flexion and extension, clinically, radiographically, and cineoradiographically. Flexion ranged from 34 to 84 degrees (average 72 degrees) and extension from 21 to 64 degrees (average 45 degrees) without helmet and shoulder pads. In well-fitting equipment flexion ranged from 36 to 86 degrees (average 73 degrees) and extension from 12 to 56 degrees(average 34 degrees). No correlation was determined between the size of the athlete's neck and the range of motion. The size of the athlete's neck was important in the determination of maximum stresses in the neck. The helmets impinged on the shoulder pads or interscapular region; this impingement diminished tension on the anterior portion of the cervical spine in extension. The face masks impinged on the shoulder pads in flexion of the neck; this impingement diminished stress on the posterior musculature. Proper fitting equipment, conditioning neck exercises, and changes in the rules of the game so that abuse of the head and neck is not encouraged are some of the aspects that may reduce the risk of injury to the cervical region in football players."}
{"id": "PMID:474866", "title": "Tennis injuries: prevention and treatment. A review.", "content": "When players are engaged in the sport of tennis, injuries may occur to the eyes, in the neck, to the shoulder and back, arm and elbow, wrist and hand, and feet. The key to prevention and treatment of these injuries is good coaching and a formal stretching and strengthening program. The drooped \"tennis shoulder\" of professionals and senior tennis players is a natural response to heavy use. Shoulder elevating exercises are useful when soreness is associated. The treatment of tennis elbow includes wrist extensor stretching, isometrics, and light weightlifting. When a player follows this program, injections or counterforce braces are rarely needed. It is important for the player to bring his racket to the examination so that his stroke mechanics and grip can be checked. Wrist soreness in a tennis player may denote a hamate hook fracture. Special radiographic views are needed to discern the fracture and it is treated with a short arm cast and little finger extension splint. Nonunion of a hamate hook requires excision. The calf pain prodrome of \"tennis leg\" requires rest and then a stretching program. Tennis shoes should have rolled heels and large toe boxes with reinforced toe bumpers. The physician may have to fashion soft inserts for the tennis shoes; arch supports may be insufficient.", "contents": "Tennis injuries: prevention and treatment. A review. When players are engaged in the sport of tennis, injuries may occur to the eyes, in the neck, to the shoulder and back, arm and elbow, wrist and hand, and feet. The key to prevention and treatment of these injuries is good coaching and a formal stretching and strengthening program. The drooped \"tennis shoulder\" of professionals and senior tennis players is a natural response to heavy use. Shoulder elevating exercises are useful when soreness is associated. The treatment of tennis elbow includes wrist extensor stretching, isometrics, and light weightlifting. When a player follows this program, injections or counterforce braces are rarely needed. It is important for the player to bring his racket to the examination so that his stroke mechanics and grip can be checked. Wrist soreness in a tennis player may denote a hamate hook fracture. Special radiographic views are needed to discern the fracture and it is treated with a short arm cast and little finger extension splint. Nonunion of a hamate hook requires excision. The calf pain prodrome of \"tennis leg\" requires rest and then a stretching program. Tennis shoes should have rolled heels and large toe boxes with reinforced toe bumpers. The physician may have to fashion soft inserts for the tennis shoes; arch supports may be insufficient."}
{"id": "PMID:474867", "title": "Eye injuries in Canadian amateur hockey.", "content": "Two studies, one retrospective (1972 to 1973) and one prospective (1974 to 1975), CONcerning eye injuries incurred by hockey players were conducted by the Canadian Ophthalmological Society with questionnaires to its members. Responses to the questionnaires were analyzed by age, type of injury, cause (i.e., hockey stick, puck, or other means), and results to visual acuity. The results were also designated by organized or unorganized participation. Almost 300 eye injuries were reported in each study. In the first study, 13.7% of the injured players became legally blind as a result of the injury; in the second study, 16% became legally blind. Organized hockey produced more injuries than unorganized hockey. The majority of the injuries were caused by the hockey stick. The injuries were both intraocular and extraocular. The group of 11- to 15-year olds received the highest number of injuries, and the older age group had the higher incidence of blindness. Studies have led to setting more rigid standards, altering rules of the game, and selecting face protectors for hockey players. Older players who care for their equipment prefer the plastic shield face protectors, and the younger players (who complain of fogging and scratching of the plastic) prefer mesh protectors through which neither the stick nor the puck can penetrate. New high sticking (above the shoulder level) rules were included in the 1976 official rule book for Canadian amateur hockey.", "contents": "Eye injuries in Canadian amateur hockey. Two studies, one retrospective (1972 to 1973) and one prospective (1974 to 1975), CONcerning eye injuries incurred by hockey players were conducted by the Canadian Ophthalmological Society with questionnaires to its members. Responses to the questionnaires were analyzed by age, type of injury, cause (i.e., hockey stick, puck, or other means), and results to visual acuity. The results were also designated by organized or unorganized participation. Almost 300 eye injuries were reported in each study. In the first study, 13.7% of the injured players became legally blind as a result of the injury; in the second study, 16% became legally blind. Organized hockey produced more injuries than unorganized hockey. The majority of the injuries were caused by the hockey stick. The injuries were both intraocular and extraocular. The group of 11- to 15-year olds received the highest number of injuries, and the older age group had the higher incidence of blindness. Studies have led to setting more rigid standards, altering rules of the game, and selecting face protectors for hockey players. Older players who care for their equipment prefer the plastic shield face protectors, and the younger players (who complain of fogging and scratching of the plastic) prefer mesh protectors through which neither the stick nor the puck can penetrate. New high sticking (above the shoulder level) rules were included in the 1976 official rule book for Canadian amateur hockey."}
{"id": "PMID:474874", "title": "Preoperative evaluation of pulmonary function.", "content": "Respiratory problems are a major cause of postoperative complications. Detection of the patient with subtle chronic obstructive pulmonary disease is essential to lessen the risk of respiratory complications in the postoperative period. In the patient with known chronic obstructive pulmonary disease, it is important to determine not only whether a lung lesion such as a neoplasm can be completely resected, but also whether the patient's cardiorespiratory reserve is adequate to allow the proposed lung resection. Detection of the patient at increased risk as well as evaluation of the ability to withstand surgery is discussed.", "contents": "Preoperative evaluation of pulmonary function. Respiratory problems are a major cause of postoperative complications. Detection of the patient with subtle chronic obstructive pulmonary disease is essential to lessen the risk of respiratory complications in the postoperative period. In the patient with known chronic obstructive pulmonary disease, it is important to determine not only whether a lung lesion such as a neoplasm can be completely resected, but also whether the patient's cardiorespiratory reserve is adequate to allow the proposed lung resection. Detection of the patient at increased risk as well as evaluation of the ability to withstand surgery is discussed."}
{"id": "PMID:474876", "title": "Anastomotic failure complicating total gastrectomy and esophagogastrectomy for cancer of the stomach.", "content": "Anastomotic failure complicated the postoperative course of 11 per cent of 350 gastric cancer patients who underwent total gastrectomy and esophagogastrectomy and was responsible for 33 per cent of all operative deaths. The extent of disease and the presence of tumor at the margin of resection did not prove to be significant factors in regard to the incidence of anastomotic failure. Gastrectomy combined with resection of other organs was associated with a significantly higher risk of failure. End-to-end esophagogastrectomy and esophagoduodenostomy appeared to be prone to failure, while Roux-en Y, jejunal pouch, and jejunal loop reconstructions were safer. Patients with severe intraabdominal or intrathoracic sepsis had a poor prognosis, and their management with surgical or conservative methods was ineffective. On the basis of these findings, alternatives to manual methods of visceral suturing should be considered.", "contents": "Anastomotic failure complicating total gastrectomy and esophagogastrectomy for cancer of the stomach. Anastomotic failure complicated the postoperative course of 11 per cent of 350 gastric cancer patients who underwent total gastrectomy and esophagogastrectomy and was responsible for 33 per cent of all operative deaths. The extent of disease and the presence of tumor at the margin of resection did not prove to be significant factors in regard to the incidence of anastomotic failure. Gastrectomy combined with resection of other organs was associated with a significantly higher risk of failure. End-to-end esophagogastrectomy and esophagoduodenostomy appeared to be prone to failure, while Roux-en Y, jejunal pouch, and jejunal loop reconstructions were safer. Patients with severe intraabdominal or intrathoracic sepsis had a poor prognosis, and their management with surgical or conservative methods was ineffective. On the basis of these findings, alternatives to manual methods of visceral suturing should be considered."}
{"id": "PMID:474877", "title": "Primary carcinoma of the gallbladder: review of 25 cases.", "content": "The records of 25 patients with carcinoma of the gallbladder occurring during a 5 year period were reviewed. The frequent relation of the disease with cholelithiasis and benign tumors has been widely recognized. Stones were present in the gallbladder in 92 per cent of the patients. In the majority of cases, a diagnosis of carcinoma of the gallbladder was not made clinically. The most common preoperative diagnosis was benign biliary tract disease. The mean survival time after operation was 7 months, and only five patients had a curative resection. The best hope for reducing the mortality from this disease lies in earlier cholecystectomy in patients with benign gallbladder disease.", "contents": "Primary carcinoma of the gallbladder: review of 25 cases. The records of 25 patients with carcinoma of the gallbladder occurring during a 5 year period were reviewed. The frequent relation of the disease with cholelithiasis and benign tumors has been widely recognized. Stones were present in the gallbladder in 92 per cent of the patients. In the majority of cases, a diagnosis of carcinoma of the gallbladder was not made clinically. The most common preoperative diagnosis was benign biliary tract disease. The mean survival time after operation was 7 months, and only five patients had a curative resection. The best hope for reducing the mortality from this disease lies in earlier cholecystectomy in patients with benign gallbladder disease."}
{"id": "PMID:474878", "title": "Pelvic exenteration for advanced rectal carcinoma.", "content": "Twelve patients with advanced rectal cancer and no evidence of extrapelvic metastases underwent pelvic exenteration. The operative mortality rate was 8.3 per cent. Determinate 3 and 5 year survival rates of 54 and 37 per cent were achieved. Criteria for the selection of patients and techniques of supravesical urinary diversion are discussed.", "contents": "Pelvic exenteration for advanced rectal carcinoma. Twelve patients with advanced rectal cancer and no evidence of extrapelvic metastases underwent pelvic exenteration. The operative mortality rate was 8.3 per cent. Determinate 3 and 5 year survival rates of 54 and 37 per cent were achieved. Criteria for the selection of patients and techniques of supravesical urinary diversion are discussed."}
{"id": "PMID:474879", "title": "Inflammatory tissue reaction in rabbit bowel injected with Crohn's homogenates.", "content": "Homogenates from the terminal ileum of a patient with Crohn's disease with granulomas were prepared as snap-frozen or fresh and were injected into the ascending colonic walls of New Zealand white rabbits. Control animals were injected with 1 per cent bovine serum albumin alone. The rabbit bowel was examined after 1 year, and lesions were noted in each of the rabbits injected with Crohn's disease homogenate, irrespective of the type of tissue preparation. The observed lesions were diffuse and occurred both at the injection site and in the terminal ileum. These changes were not noted in the control group. This work confirms earlier results in the same animal model and suggests that either fresh or snap-frozen homogenates will produce the intestinal lesion but that bovine albumin alone will not.", "contents": "Inflammatory tissue reaction in rabbit bowel injected with Crohn's homogenates. Homogenates from the terminal ileum of a patient with Crohn's disease with granulomas were prepared as snap-frozen or fresh and were injected into the ascending colonic walls of New Zealand white rabbits. Control animals were injected with 1 per cent bovine serum albumin alone. The rabbit bowel was examined after 1 year, and lesions were noted in each of the rabbits injected with Crohn's disease homogenate, irrespective of the type of tissue preparation. The observed lesions were diffuse and occurred both at the injection site and in the terminal ileum. These changes were not noted in the control group. This work confirms earlier results in the same animal model and suggests that either fresh or snap-frozen homogenates will produce the intestinal lesion but that bovine albumin alone will not."}
{"id": "PMID:474881", "title": "Tumor variation in the cancer family syndrome: ovarian cancer.", "content": "The Cancer Family Syndrome is a hereditary disorder (autosomal dominant), characterized by early onset cancer of the colon (particularly the proximal colon) and endometrium, with an excess of multiple primary cancers. Recent evidence reflects the possibility of an even broader tumor spectrum consisting of carcinoma of the stomach, the ovary, the kidney, and possibly other organs. A family with the cardinal features of the Cancer Family Syndrome is described, including two sisters and their mother who had ovarian carcinoma at early ages (38, 46, and 49 years). Two of these three women have shown unusual tolerance to cancer, despite invasion of the primary tumors. Implications for cancer surveillance and management are discussed.", "contents": "Tumor variation in the cancer family syndrome: ovarian cancer. The Cancer Family Syndrome is a hereditary disorder (autosomal dominant), characterized by early onset cancer of the colon (particularly the proximal colon) and endometrium, with an excess of multiple primary cancers. Recent evidence reflects the possibility of an even broader tumor spectrum consisting of carcinoma of the stomach, the ovary, the kidney, and possibly other organs. A family with the cardinal features of the Cancer Family Syndrome is described, including two sisters and their mother who had ovarian carcinoma at early ages (38, 46, and 49 years). Two of these three women have shown unusual tolerance to cancer, despite invasion of the primary tumors. Implications for cancer surveillance and management are discussed."}
{"id": "PMID:474882", "title": "Long-term cimetidine and anticholinergic therapy in patients with gastrinoma.", "content": "Two patients with gastrinomas were treated with cimetidine and an anticholinergic drug for greater than 20 months after exploratory laparotomy disclosed no solitary resectable tumor. Both have done well, and thus this therapy is recommended as a safe and effective alternative to total gastrectomy in reliable patients. Additional therapeutic methods are needed to control tumor growth.", "contents": "Long-term cimetidine and anticholinergic therapy in patients with gastrinoma. Two patients with gastrinomas were treated with cimetidine and an anticholinergic drug for greater than 20 months after exploratory laparotomy disclosed no solitary resectable tumor. Both have done well, and thus this therapy is recommended as a safe and effective alternative to total gastrectomy in reliable patients. Additional therapeutic methods are needed to control tumor growth."}
{"id": "PMID:474884", "title": "Effect of surgical scrub on microbial population under the fingernails.", "content": "The effectiveness of two methods of presurgical hand preparation, the 10 minute routine scrub and the 90 second Hydroscrub, in reducing microbial numbers under the fingernails was determined. Bacteriologic cultures of 162 subungual areas of nine subjects revealed prescrub microbial counts of up to 1.9 X 10(5) colony-forming units per area. After the surgical scrub, bacterial concentrations were reduced to a different degree among the persons tested. The mean values of 3.0 X 10(3) to 7.8 X 10(3) colony-forming units immediately after scrub indicated that neither of the two methods tested reduced the microbial population under the fingernails of most persons to acceptable levels. The finding of high postscrub microbial counts under the fingernails is of particular interest and significance in view of the relatively low counts on the fingertips. On the basis of the results of this study, we conclude that degerming of the areas under the fingernails by present methods is not satisfactory. The implication of these findings is discussed.", "contents": "Effect of surgical scrub on microbial population under the fingernails. The effectiveness of two methods of presurgical hand preparation, the 10 minute routine scrub and the 90 second Hydroscrub, in reducing microbial numbers under the fingernails was determined. Bacteriologic cultures of 162 subungual areas of nine subjects revealed prescrub microbial counts of up to 1.9 X 10(5) colony-forming units per area. After the surgical scrub, bacterial concentrations were reduced to a different degree among the persons tested. The mean values of 3.0 X 10(3) to 7.8 X 10(3) colony-forming units immediately after scrub indicated that neither of the two methods tested reduced the microbial population under the fingernails of most persons to acceptable levels. The finding of high postscrub microbial counts under the fingernails is of particular interest and significance in view of the relatively low counts on the fingertips. On the basis of the results of this study, we conclude that degerming of the areas under the fingernails by present methods is not satisfactory. The implication of these findings is discussed."}
{"id": "PMID:474944", "title": "Central dopamine receptors regulate blood eosinophilia in the rat.", "content": "Dopaminergic agents, dopa and apomorphine, affected biphasically the blood eosinophil count in the rat: low doses of the drug elevated, while high doses lowered it. The response to a high dose of dopa was retained in rats pretreated with an inhibitor of dopamine-beta-hydroxylase, U 10, 157, but prevented by a centrally acting dopa decarboxylase inhibitor, NSD 1015. This indicates that the eosinopenia observed after large doses of dopa is due to the action of dopamine formed from the precursor. As intracerebroventricular injections of Ldopa also produce eosinopenia, the central site of dopamine action is indicated. The eosinopenic response to apomorphine was antagonized by a dopamine receptor blocking agent, haloperidol. This indicates that some central dopamine  receptors are involved in the regulation of the eosinophil count in circulating blood. The hypophysis seems to play a crucial role in the phenomenon observed, as no eosinopenia was produced by dopa in hypophysectomized rats.", "contents": "Central dopamine receptors regulate blood eosinophilia in the rat. Dopaminergic agents, dopa and apomorphine, affected biphasically the blood eosinophil count in the rat: low doses of the drug elevated, while high doses lowered it. The response to a high dose of dopa was retained in rats pretreated with an inhibitor of dopamine-beta-hydroxylase, U 10, 157, but prevented by a centrally acting dopa decarboxylase inhibitor, NSD 1015. This indicates that the eosinopenia observed after large doses of dopa is due to the action of dopamine formed from the precursor. As intracerebroventricular injections of Ldopa also produce eosinopenia, the central site of dopamine action is indicated. The eosinopenic response to apomorphine was antagonized by a dopamine receptor blocking agent, haloperidol. This indicates that some central dopamine  receptors are involved in the regulation of the eosinophil count in circulating blood. The hypophysis seems to play a crucial role in the phenomenon observed, as no eosinopenia was produced by dopa in hypophysectomized rats."}
{"id": "PMID:474947", "title": "Intravenous administration of glycopyrronium: effects on cardiac rate and rhythm.", "content": "Intravenous administration of glycopyrronium in doses up to 0.2 mg, was associated with minimal changes in cardiac rate and rhythm. Sinus arrhythmia, nodal rhythm and occasional atrial ectopics were the dysrhythmias observed. There were no accompanying changes in blood pressure.", "contents": "Intravenous administration of glycopyrronium: effects on cardiac rate and rhythm. Intravenous administration of glycopyrronium in doses up to 0.2 mg, was associated with minimal changes in cardiac rate and rhythm. Sinus arrhythmia, nodal rhythm and occasional atrial ectopics were the dysrhythmias observed. There were no accompanying changes in blood pressure."}
{"id": "PMID:474948", "title": "Analgesic effects of sublingual buprenorphine.", "content": "The analgesic effects of sublingually administered buprenorphine 0.4 mg have been compared with morphine 10 mg given intramuscularly in patients following operation. The results indicate a slower onset of action for buprenorphine but of much longer duration than morphine. There were no serious side effects or difference in their incidence between the two drugs.", "contents": "Analgesic effects of sublingual buprenorphine. The analgesic effects of sublingually administered buprenorphine 0.4 mg have been compared with morphine 10 mg given intramuscularly in patients following operation. The results indicate a slower onset of action for buprenorphine but of much longer duration than morphine. There were no serious side effects or difference in their incidence between the two drugs."}
{"id": "PMID:474949", "title": "The evaluation of analgesic drugs. The case for experimental methods.", "content": "The current status of the experimental, as opposed to the traditional clinical, approach to the evaluation of analgesic activity is examined in the light of recent observations made with the submaximal tourniquet and electric shock methods of inducing experimental pain. The results obtained indicate that the submaximal effort tourniquet technique shows an acceptable degree of sensitivity in detecting the analgesic activity of both narcotic and non-narcotic agents which is not the case with the electric shock method. These differences in sensitivities between two different experimental methods are examined and some possible explanations offered for the findings. It is concluded that some modern experimental methods may now provide a valid and desirable alternative to the clinical approach for the evaluation of analgesic agents.", "contents": "The evaluation of analgesic drugs. The case for experimental methods. The current status of the experimental, as opposed to the traditional clinical, approach to the evaluation of analgesic activity is examined in the light of recent observations made with the submaximal tourniquet and electric shock methods of inducing experimental pain. The results obtained indicate that the submaximal effort tourniquet technique shows an acceptable degree of sensitivity in detecting the analgesic activity of both narcotic and non-narcotic agents which is not the case with the electric shock method. These differences in sensitivities between two different experimental methods are examined and some possible explanations offered for the findings. It is concluded that some modern experimental methods may now provide a valid and desirable alternative to the clinical approach for the evaluation of analgesic agents."}
{"id": "PMID:474950", "title": "Accidental intra-arterial injection of papaveretum during anaesthesia.", "content": "The circulatory depressant action of papaveretum was demonstrated when the drug was injected accidentally into the radial artery during neuroanaesthesia in the upright (sitting) position. The signs of air embolism were mimicked. The condition reponded promptly to a vasopressor given intravenously. There were no harmful sequelae to the patient's hand.", "contents": "Accidental intra-arterial injection of papaveretum during anaesthesia. The circulatory depressant action of papaveretum was demonstrated when the drug was injected accidentally into the radial artery during neuroanaesthesia in the upright (sitting) position. The signs of air embolism were mimicked. The condition reponded promptly to a vasopressor given intravenously. There were no harmful sequelae to the patient's hand."}
{"id": "PMID:474951", "title": "Valve replacement in carcinoid syndrome. Anaesthetic management for tricuspid and pulmonary valve surgery.", "content": "This report describes the anaesthetic management of a patient with carcinoid syndrome for cardiac catheterisation followed by replacement of the tricuspid and pulmonary valves. Apart from the precautionary use of aprotinin and steroids, routine techniques of anaesthesia and monitoring were used without complications attributable to secretions from tumour tissue.", "contents": "Valve replacement in carcinoid syndrome. Anaesthetic management for tricuspid and pulmonary valve surgery. This report describes the anaesthetic management of a patient with carcinoid syndrome for cardiac catheterisation followed by replacement of the tricuspid and pulmonary valves. Apart from the precautionary use of aprotinin and steroids, routine techniques of anaesthesia and monitoring were used without complications attributable to secretions from tumour tissue."}
{"id": "PMID:474952", "title": "Errors in fuel cell oxygen analysers during NEEP.", "content": "A fuel cell oxygen analyser was found to underread during the application of negative end-expiratory pressure. Further investigation revealed that air leaks into the analyser head through the plastic housing and causes the analyser to read low. A remedy is suggested.", "contents": "Errors in fuel cell oxygen analysers during NEEP. A fuel cell oxygen analyser was found to underread during the application of negative end-expiratory pressure. Further investigation revealed that air leaks into the analyser head through the plastic housing and causes the analyser to read low. A remedy is suggested."}
{"id": "PMID:474953", "title": "High tracheal bifurcation.", "content": "A case study is reported, outlining abnormal tracheobronchial anatomy in an adult male presented for thoractomy. The anesthetic technique is described. Ureteric catheters were used as introducers for the selective intubation of each main bronchus. The problems encountered postoperatively are also discussed.", "contents": "High tracheal bifurcation. A case study is reported, outlining abnormal tracheobronchial anatomy in an adult male presented for thoractomy. The anesthetic technique is described. Ureteric catheters were used as introducers for the selective intubation of each main bronchus. The problems encountered postoperatively are also discussed."}
{"id": "PMID:474954", "title": "The nature and incidence of complications of peripheral arterial puncture.", "content": "Follow-up of the sites of 839 peripheral arterial punctures has confirmed the technique as safe and free from major sequelae. Minor complications, mainly bruising and tenderness, occurred at 39% of sites; this incidence is much less than that previously reported following short-term arterial cannulation. At only 2% of sites was there any significant pain or tenderness. There was a small incidence of pulse diminution particularly at the radial site but no case of arterial occlusion. The use of a smaller needle (23 s.w.g.) significantly reduced the overall incidence of complications.", "contents": "The nature and incidence of complications of peripheral arterial puncture. Follow-up of the sites of 839 peripheral arterial punctures has confirmed the technique as safe and free from major sequelae. Minor complications, mainly bruising and tenderness, occurred at 39% of sites; this incidence is much less than that previously reported following short-term arterial cannulation. At only 2% of sites was there any significant pain or tenderness. There was a small incidence of pulse diminution particularly at the radial site but no case of arterial occlusion. The use of a smaller needle (23 s.w.g.) significantly reduced the overall incidence of complications."}
{"id": "PMID:474955", "title": "Total intravenous anaesthesia for computerised axial tomography.", "content": "A method of anaesthetising patients for computerised axial tomography using an intravenous infusion of alphaxalone/alphadolone as the sole agent is described. Its successful use in 120 patients of all ages has led to a better understanding of the mode of action of the drug and suggestions are made about its use in more general anaesthetic practice.", "contents": "Total intravenous anaesthesia for computerised axial tomography. A method of anaesthetising patients for computerised axial tomography using an intravenous infusion of alphaxalone/alphadolone as the sole agent is described. Its successful use in 120 patients of all ages has led to a better understanding of the mode of action of the drug and suggestions are made about its use in more general anaesthetic practice."}
{"id": "PMID:474979", "title": "Negative surplus charge of coated erythrocytes.", "content": "The effect on the net negative surface charge of single or multiple coating of erythrocytes was studied on human group A red cells previously washed and fixed. Single coating with either group A antiserum or protectin decreased the electrophoretic mobility of erythrocytes by 16% and 13%, respectively. Following an incubation in group A glycophorin or bovine submaxillary mucin of agglutinin coated cells, the electrophoretic mobility was restored or slightly increased compared with uncoated erythrocytes. Upon repeating this scheme, the second agglutinin binding again reduced these cells' cytophoretic mobility, and they were accelerated once more by a second coating with acid glycoprotein. Intensity and homogeneity of colloidal iron staining of uncoated and variously coated erythrocytes reflected their electrophoretic retardation and acceleration, respectively.", "contents": "Negative surplus charge of coated erythrocytes. The effect on the net negative surface charge of single or multiple coating of erythrocytes was studied on human group A red cells previously washed and fixed. Single coating with either group A antiserum or protectin decreased the electrophoretic mobility of erythrocytes by 16% and 13%, respectively. Following an incubation in group A glycophorin or bovine submaxillary mucin of agglutinin coated cells, the electrophoretic mobility was restored or slightly increased compared with uncoated erythrocytes. Upon repeating this scheme, the second agglutinin binding again reduced these cells' cytophoretic mobility, and they were accelerated once more by a second coating with acid glycoprotein. Intensity and homogeneity of colloidal iron staining of uncoated and variously coated erythrocytes reflected their electrophoretic retardation and acceleration, respectively."}
{"id": "PMID:474980", "title": "[The morphology of the lacrimal gland of Herpestes edwardsi (Viverridae) (author's transl)].", "content": "The morphology from the Glandula lacrimalis of the mongoose Herpestis edwardsi is described. Light microscopical observations show that the acini consist of ramified, mucous tubules and of serous demilunes (Fig. 1, 2). Thus the morphology of this organ of the mongoose differs from those of other species remarkably. Electron microscopical the demilunes and the tubules show characteristical granula which arise from Golgi-vesicles. The granula fuse and eliminate their contents into the clearing by exocytosis. In the demilunes there are granula of different size and shape. Their contents aremostly dense and cristallize sometimes to rod-shaped, lamellar structures (Fig. 5). The cells of the tubules contain abundant bizonal granula which seem to be in contact with the rER (Figs. 6,7). The epithel of the intra- and interlobular ducts includes membran-bounded osmiophilic substances. The cytoplasm of the cells is enforced by filaments which aggregate beneath the apical plasmalemma to a prominant terminal web-like layer (Fig. 9). Intercalated and striated ducts are lacking. The results are discussed with earlier findings at the Glandula lacrimalis of other species.", "contents": "[The morphology of the lacrimal gland of Herpestes edwardsi (Viverridae) (author's transl)]. The morphology from the Glandula lacrimalis of the mongoose Herpestis edwardsi is described. Light microscopical observations show that the acini consist of ramified, mucous tubules and of serous demilunes (Fig. 1, 2). Thus the morphology of this organ of the mongoose differs from those of other species remarkably. Electron microscopical the demilunes and the tubules show characteristical granula which arise from Golgi-vesicles. The granula fuse and eliminate their contents into the clearing by exocytosis. In the demilunes there are granula of different size and shape. Their contents aremostly dense and cristallize sometimes to rod-shaped, lamellar structures (Fig. 5). The cells of the tubules contain abundant bizonal granula which seem to be in contact with the rER (Figs. 6,7). The epithel of the intra- and interlobular ducts includes membran-bounded osmiophilic substances. The cytoplasm of the cells is enforced by filaments which aggregate beneath the apical plasmalemma to a prominant terminal web-like layer (Fig. 9). Intercalated and striated ducts are lacking. The results are discussed with earlier findings at the Glandula lacrimalis of other species."}
{"id": "PMID:474981", "title": "[The three-dimensional ultrastructure of the pancreatic acinar cell (authors transl)].", "content": "The pancreatic acinar cells of normal rats were studied by scanning electron microscope. A three-dimensional image is presented of the structure and arrangement of the zymogen granules as well as of the apical microvilli and the zymogen discharge in the pancreatic acinar cell. The study of these structures in physiologic and pathologic conditions by scanning electron microscopy can be recommended to support the understanding of findings obtained by light and transmission electron microscopy.", "contents": "[The three-dimensional ultrastructure of the pancreatic acinar cell (authors transl)]. The pancreatic acinar cells of normal rats were studied by scanning electron microscope. A three-dimensional image is presented of the structure and arrangement of the zymogen granules as well as of the apical microvilli and the zymogen discharge in the pancreatic acinar cell. The study of these structures in physiologic and pathologic conditions by scanning electron microscopy can be recommended to support the understanding of findings obtained by light and transmission electron microscopy."}
{"id": "PMID:474982", "title": "Morphology of the hypophysis of the freshwater teleost rhamdia hilarii (val., 1840).", "content": "The anatomical relationship between hypophysis and encephalon of the Bagre fish (Rhamdia hilarii, Val.) was studied. The subdivisions, morphology and the relative volume (%) of the different hypophyseal regions during the reproductive stage of the fish were also considered.", "contents": "Morphology of the hypophysis of the freshwater teleost rhamdia hilarii (val., 1840). The anatomical relationship between hypophysis and encephalon of the Bagre fish (Rhamdia hilarii, Val.) was studied. The subdivisions, morphology and the relative volume (%) of the different hypophyseal regions during the reproductive stage of the fish were also considered."}
{"id": "PMID:474983", "title": "Ratio of rods and different cones in fishes of shallow sea.", "content": "The retinas of 4 fishes (Periophthalmus chrysopilos, Scorpaenoides marmoratus, Nemipterus bathybins, Taius tumifrons) were analysed. Rods were found to increase with depth but twin cones were not found to descrease with depth. A regional difference was also observed in the distribution of these elements in the retinas.", "contents": "Ratio of rods and different cones in fishes of shallow sea. The retinas of 4 fishes (Periophthalmus chrysopilos, Scorpaenoides marmoratus, Nemipterus bathybins, Taius tumifrons) were analysed. Rods were found to increase with depth but twin cones were not found to descrease with depth. A regional difference was also observed in the distribution of these elements in the retinas."}
{"id": "PMID:474984", "title": "Arterial pattern of the spleen in the domestic fowl.", "content": "The authors describe the variations on the origin, number and vascularization areas of the splenic artery of 50 fowls, 34 males and 16 females, Gallus gallus, Indian River, of different ages, with medium weight of 2.133 g. The results were expressed in relative percentage figures and compared with the literature cited.", "contents": "Arterial pattern of the spleen in the domestic fowl. The authors describe the variations on the origin, number and vascularization areas of the splenic artery of 50 fowls, 34 males and 16 females, Gallus gallus, Indian River, of different ages, with medium weight of 2.133 g. The results were expressed in relative percentage figures and compared with the literature cited."}
{"id": "PMID:474985", "title": "Macroscopic anatomy of the bronchial arteries.", "content": "Exact and detailed findings concerning a macroscopic anatomy of the bronchial arteries have not been presented. Moreover, positional relations of this artery to the adjacent organs had mostly been neglected, which offered an important key to identify the aberrant vessel in some cardiac malformations. The authors dissected the bronchial arteries from their origin to the pulmonary hilus in 40 Jpanese adult cadavers. Against the descriptions of textbooks of anatomy, 2 right and 2 left arteries in a body were most commonly observed. Therefore, 4 arteries could be distinguished, right superior, right inferior, left superior and left inferior, which passed along cranial and caudal borders of the corresponding bronchus respectively. Origins of these arteries were 1) right aortic intercostal artery, 2) right subclavian artery, 3) left subclavian artery, 4) aortic arch proximal to the left recurrent laryngeal nerve and 5) descending aorta distal to the above-mentioned nerve. As a rule, each of the 4 arteries above had a definite origin and course, as shown in Fig. 7. Postional relations of these arteries to the trachea, esophagus, thoracic duct, azygos vein and the left recurrent laryngeal nerve were also described.", "contents": "Macroscopic anatomy of the bronchial arteries. Exact and detailed findings concerning a macroscopic anatomy of the bronchial arteries have not been presented. Moreover, positional relations of this artery to the adjacent organs had mostly been neglected, which offered an important key to identify the aberrant vessel in some cardiac malformations. The authors dissected the bronchial arteries from their origin to the pulmonary hilus in 40 Jpanese adult cadavers. Against the descriptions of textbooks of anatomy, 2 right and 2 left arteries in a body were most commonly observed. Therefore, 4 arteries could be distinguished, right superior, right inferior, left superior and left inferior, which passed along cranial and caudal borders of the corresponding bronchus respectively. Origins of these arteries were 1) right aortic intercostal artery, 2) right subclavian artery, 3) left subclavian artery, 4) aortic arch proximal to the left recurrent laryngeal nerve and 5) descending aorta distal to the above-mentioned nerve. As a rule, each of the 4 arteries above had a definite origin and course, as shown in Fig. 7. Postional relations of these arteries to the trachea, esophagus, thoracic duct, azygos vein and the left recurrent laryngeal nerve were also described."}
{"id": "PMID:474986", "title": "[The distribution of sensory and motor axons of the intercostal nerves and their branches (author's transl)].", "content": "As it is well established that motor axons in general display a higher acetylcholinesterase-activity than sensory axons do, the histochemical method of KARNOVSKY and ROOTS (1964) was used for the differentiation of motor and sensory fibres in the intercostal nerves and their branches. In the paravertebral sections of the intercostal nerves of the upper segments 30--35% of the nerve fibres show a high enzyme activity and therefore were classified as motoric. The percentage of the motor fibres in comparable zones of the lower segments increases to 45%. Only 15% of the nerve fibres proved to be motoric in the parasternal sections of the intercostal nerves. In a histogram of the acetylcholinesterase-positive intercostal nerve fibres 2 peaks can be seen: one in the alpha-calibre class, the second in the gamma-class. There are more motor axons in the lateral cutaneous branch of the lower intercostal nerve than in upper ones. This may be explained by the participation of these nerve branches in the innervation of the abdominal muscles. In 2 cases nerve branches of the intercostal nerve to the diaphragm were found containing 15--25% motor axons.", "contents": "[The distribution of sensory and motor axons of the intercostal nerves and their branches (author's transl)]. As it is well established that motor axons in general display a higher acetylcholinesterase-activity than sensory axons do, the histochemical method of KARNOVSKY and ROOTS (1964) was used for the differentiation of motor and sensory fibres in the intercostal nerves and their branches. In the paravertebral sections of the intercostal nerves of the upper segments 30--35% of the nerve fibres show a high enzyme activity and therefore were classified as motoric. The percentage of the motor fibres in comparable zones of the lower segments increases to 45%. Only 15% of the nerve fibres proved to be motoric in the parasternal sections of the intercostal nerves. In a histogram of the acetylcholinesterase-positive intercostal nerve fibres 2 peaks can be seen: one in the alpha-calibre class, the second in the gamma-class. There are more motor axons in the lateral cutaneous branch of the lower intercostal nerve than in upper ones. This may be explained by the participation of these nerve branches in the innervation of the abdominal muscles. In 2 cases nerve branches of the intercostal nerve to the diaphragm were found containing 15--25% motor axons."}
{"id": "PMID:474988", "title": "[Histological investigations on adrenal cortex of sand rats (Psammomys obesus) during development of the diabetes mellitus (author's transl)].", "content": "The investigations were carried out on 20 sand rats (6 prediabetic, 6 diabetic and 8 normoglycemic animals). The cortices of adrenal glands were studied with histological methods. The thickness of the zona glomerulosa is in this 3 groups different. In the prediabetic group is the zona glomerulosa higher, in the diabetic group lower than the normoglycemic group. The relationship is 1.5 (normoglycemic group) : 2.9 (prediabetic group) : 0.8 (diabetic group). It is concluded that the zona glomerulosa in the prediabetic group is stimulated.", "contents": "[Histological investigations on adrenal cortex of sand rats (Psammomys obesus) during development of the diabetes mellitus (author's transl)]. The investigations were carried out on 20 sand rats (6 prediabetic, 6 diabetic and 8 normoglycemic animals). The cortices of adrenal glands were studied with histological methods. The thickness of the zona glomerulosa is in this 3 groups different. In the prediabetic group is the zona glomerulosa higher, in the diabetic group lower than the normoglycemic group. The relationship is 1.5 (normoglycemic group) : 2.9 (prediabetic group) : 0.8 (diabetic group). It is concluded that the zona glomerulosa in the prediabetic group is stimulated."}
{"id": "PMID:474989", "title": "Histomorphological and histochemical researches on the digestive tract of the freshwater grass carp, Ctenopharyngodon idella (cypriniformes).", "content": "Ctenopharyngodon idella does not have a stomach, as is shown by the absence of gastric glands. The intestine is a simple coiled tube about twice the length of the body, having no valves and no ceca, and it is joined directly to the esophagus. The mucosa is fairly uniform in appearance, being thrown into folds which give it a mesh-like appearance. No multicellular glands can be detected in any part of the alimentary canal. Goblet cells are present: no differences are found in young specimens. Mucous in the goblet cells from young animals shows no differences in the various parts of the intestine. In the adult animals the mucous is markedly acidic in the first part of the intestine. In the young animals some migrant cells are present in the mucosa and in the connective tissue; these being more numerous in the fingerlings of 6--7 cm in length.", "contents": "Histomorphological and histochemical researches on the digestive tract of the freshwater grass carp, Ctenopharyngodon idella (cypriniformes). Ctenopharyngodon idella does not have a stomach, as is shown by the absence of gastric glands. The intestine is a simple coiled tube about twice the length of the body, having no valves and no ceca, and it is joined directly to the esophagus. The mucosa is fairly uniform in appearance, being thrown into folds which give it a mesh-like appearance. No multicellular glands can be detected in any part of the alimentary canal. Goblet cells are present: no differences are found in young specimens. Mucous in the goblet cells from young animals shows no differences in the various parts of the intestine. In the adult animals the mucous is markedly acidic in the first part of the intestine. In the young animals some migrant cells are present in the mucosa and in the connective tissue; these being more numerous in the fingerlings of 6--7 cm in length."}
{"id": "PMID:474990", "title": "Structure of the esophagus in the adult opossum, Didelphis virginiana.", "content": "The structure of the esophagus has been studied in the adult opossum, Didelphis virginiana. A thickening of both layers of the muscularis externa occurs at the origin of the esophagus and may represent the upper esophageal sphincter; a massive expansion of the muscularis mucosae occurs in the region of the lower esophageal sphincter. The distribution of striated, mixed and smooth muscle in the muscularis externa differs in the inner and outer layers and elements of the myenteric plexus are found to occur even in the region of striated muscle; however, the ganglia of this plexus become much more prominent as smooth muscle makes its appearance. Esophageal glands are found in the lamina propria where they are confined to the 2 ends. They are especially prominent at the distal end where they are responsible for the formation of permanent transverse folds. Similar glands are found in the submucosa, scattered throughout the length of the esophagus but distally, in the region of the transverse folds, the submucous glands disappear. In both of these layers, the glands contain mucous, serous and myoepithelial cells.", "contents": "Structure of the esophagus in the adult opossum, Didelphis virginiana. The structure of the esophagus has been studied in the adult opossum, Didelphis virginiana. A thickening of both layers of the muscularis externa occurs at the origin of the esophagus and may represent the upper esophageal sphincter; a massive expansion of the muscularis mucosae occurs in the region of the lower esophageal sphincter. The distribution of striated, mixed and smooth muscle in the muscularis externa differs in the inner and outer layers and elements of the myenteric plexus are found to occur even in the region of striated muscle; however, the ganglia of this plexus become much more prominent as smooth muscle makes its appearance. Esophageal glands are found in the lamina propria where they are confined to the 2 ends. They are especially prominent at the distal end where they are responsible for the formation of permanent transverse folds. Similar glands are found in the submucosa, scattered throughout the length of the esophagus but distally, in the region of the transverse folds, the submucous glands disappear. In both of these layers, the glands contain mucous, serous and myoepithelial cells."}
{"id": "PMID:474991", "title": "Morphological events occuring in the seminiferous tubules of the Brazilian Nelore zebu associated with puberty.", "content": "The morphological events occurring in the Brazilian Nelore zebu testis from 3 to 18 months of age are described. Spermatozoa were seen only at 16--18 months of age indicating a considerable delay in the sexual development of the Nelore zebu when compared with taurine breeds.", "contents": "Morphological events occuring in the seminiferous tubules of the Brazilian Nelore zebu associated with puberty. The morphological events occurring in the Brazilian Nelore zebu testis from 3 to 18 months of age are described. Spermatozoa were seen only at 16--18 months of age indicating a considerable delay in the sexual development of the Nelore zebu when compared with taurine breeds."}
{"id": "PMID:474992", "title": "[Increase functions of the type dW/dt = ktp-1(tEp--tp)q and their integrals (author's transl)].", "content": "After the investigations into 4 increase functions of organic growth a fifth ansatz should be added as dW/dt = ktp(tE--t)q with only the time t involved on the right side of the equation. In the course of researches about approximations the author has dealed with this type of functions, which can only be integrated within the limits t = 0 and t = tE leading to the Beta-function with W = E as a restricted result. Therefore the basic equation was thoroughly modified as to fit the type dW/dt = cf(t) df(t)/dt. Surprisingly the integration results in a growth function formally equal to the solution for the increase function dW/dt = ktp(E--W)n. Although the determination of the coefficients runs along other lines, the traditional example given in the preceding papers shows values so close to each other as to suppose genuine identity, which is finally proved. The fact, that seemingly quite different increase functions can lead to identical results must be given serious account: judging about increase functions should be taken as a rather delicate matter.", "contents": "[Increase functions of the type dW/dt = ktp-1(tEp--tp)q and their integrals (author's transl)]. After the investigations into 4 increase functions of organic growth a fifth ansatz should be added as dW/dt = ktp(tE--t)q with only the time t involved on the right side of the equation. In the course of researches about approximations the author has dealed with this type of functions, which can only be integrated within the limits t = 0 and t = tE leading to the Beta-function with W = E as a restricted result. Therefore the basic equation was thoroughly modified as to fit the type dW/dt = cf(t) df(t)/dt. Surprisingly the integration results in a growth function formally equal to the solution for the increase function dW/dt = ktp(E--W)n. Although the determination of the coefficients runs along other lines, the traditional example given in the preceding papers shows values so close to each other as to suppose genuine identity, which is finally proved. The fact, that seemingly quite different increase functions can lead to identical results must be given serious account: judging about increase functions should be taken as a rather delicate matter."}
{"id": "PMID:474993", "title": "[Relationships between the height of intervertebral discs and the degree of lordosis of the cervical spine in children. A roentgenometric study (author's transl)].", "content": "On lateral roentgenograms of the cervical spine of 120 normal children between 3 and 14 years of age the ventral and dorsal height of all 5 intervertebral discs were measured and the \"index of the intervertebral disc\" was calculated. In addition, the \"degree of lordosis\" of the cervical spine was determined according to ISHIHARA (1964). Evaluation was in 3 age groups: 3 to 6, 7 to 10, 11 to 14 years; and the arithmetic mean and standard deviation were calculated for each group. All intervertebral discs are more or less \"cuneiform\" (ventrally higher than dorsally); they narrow from cranial to caudal. In children the degree of lordosis of the cervical spine shows a continuous decrease with advancing years. In all age groups girls show a lower degree of lordosis than boys. Statistically the differences of average values of the intervertebral-disc-index among the various shapes of the cervical spine in neutral position (straight, sublordotic, lordotic and hyperlordotic) are highly significant. By means of roentgenometric methods our examinations demonstrate that the lordotic curvature of the healthy cervical spine primarily depends on the cuneiform shape of intervertebral discs.", "contents": "[Relationships between the height of intervertebral discs and the degree of lordosis of the cervical spine in children. A roentgenometric study (author's transl)]. On lateral roentgenograms of the cervical spine of 120 normal children between 3 and 14 years of age the ventral and dorsal height of all 5 intervertebral discs were measured and the \"index of the intervertebral disc\" was calculated. In addition, the \"degree of lordosis\" of the cervical spine was determined according to ISHIHARA (1964). Evaluation was in 3 age groups: 3 to 6, 7 to 10, 11 to 14 years; and the arithmetic mean and standard deviation were calculated for each group. All intervertebral discs are more or less \"cuneiform\" (ventrally higher than dorsally); they narrow from cranial to caudal. In children the degree of lordosis of the cervical spine shows a continuous decrease with advancing years. In all age groups girls show a lower degree of lordosis than boys. Statistically the differences of average values of the intervertebral-disc-index among the various shapes of the cervical spine in neutral position (straight, sublordotic, lordotic and hyperlordotic) are highly significant. By means of roentgenometric methods our examinations demonstrate that the lordotic curvature of the healthy cervical spine primarily depends on the cuneiform shape of intervertebral discs."}
{"id": "PMID:474994", "title": "[Variations in the pathway of the inferior gluteal nerve (author's transl)].", "content": "In 17 out of 112 subjects examined, the inferior gluteal nerve leaves the pelvis through the piriformis and passes to the gluteus maximus. In 3 of the subjects the variation occurs bilaterally, and in 7 cases each it is found on either the right or the left side of the body alone. This variation occurs more often in women than in men. In all cases observed the common peroneal nerve also leaves the pelvis through the piriformis. Aside from the common peroneal nerve, the entire posterior femoral cutaneous nerve, or only its dorsal ramus, often runs alongside the inferior gluteal nerve through the \"foramen intrapiriforme\". The origin of this variations has to be discussed in connection with the specific embryological development of the piriformis.", "contents": "[Variations in the pathway of the inferior gluteal nerve (author's transl)]. In 17 out of 112 subjects examined, the inferior gluteal nerve leaves the pelvis through the piriformis and passes to the gluteus maximus. In 3 of the subjects the variation occurs bilaterally, and in 7 cases each it is found on either the right or the left side of the body alone. This variation occurs more often in women than in men. In all cases observed the common peroneal nerve also leaves the pelvis through the piriformis. Aside from the common peroneal nerve, the entire posterior femoral cutaneous nerve, or only its dorsal ramus, often runs alongside the inferior gluteal nerve through the \"foramen intrapiriforme\". The origin of this variations has to be discussed in connection with the specific embryological development of the piriformis."}
{"id": "PMID:474995", "title": "[Doubling of the arteria testicularis sinistra with variations of origin and course--an embryological consideration (author's transl)].", "content": "During dissection of a human body anomalies in the origin and the course of the testicular artery have been observed. Besides a left testicular artery arching over the left renal vein there existed an additional testicular artery on the same side originating from the distal part of the left renal artery. The development of those anomalies is discussed under embryological aspects.", "contents": "[Doubling of the arteria testicularis sinistra with variations of origin and course--an embryological consideration (author's transl)]. During dissection of a human body anomalies in the origin and the course of the testicular artery have been observed. Besides a left testicular artery arching over the left renal vein there existed an additional testicular artery on the same side originating from the distal part of the left renal artery. The development of those anomalies is discussed under embryological aspects."}
{"id": "PMID:474997", "title": "In vitro studies on the self-differentiating capacities of quail adenohypophysis epithelium.", "content": "Isolated epithelial rudiments of 3--4 days quail embryo adenohypophysis were cultivated in vitro. Differentiation of glandular cells occurred when culture conditions allowed the formation of explants characterized by the presence of a fibroblastic sheet and of an epithelial roof surrounding a central cavity. Differentiation did not occur when culture conditions did not allow the fibroblastic sheet to be established or resulted in the absence of a cavity. The importance of the explant structure in differentiation is also indicated by the location of glandular cells. In vitro they differentiate near the cavity derived from Rathke's pouch lumen while in situ they first appear at the periphery of the rudiment, near the basement membrane. The results of this study show that the adenohypophyseal primordium can differentiate without any mesenchymal influence. The observed differentiation seems to be correlated with fibroblast-secreted material, and this hypothesis is discussed.", "contents": "In vitro studies on the self-differentiating capacities of quail adenohypophysis epithelium. Isolated epithelial rudiments of 3--4 days quail embryo adenohypophysis were cultivated in vitro. Differentiation of glandular cells occurred when culture conditions allowed the formation of explants characterized by the presence of a fibroblastic sheet and of an epithelial roof surrounding a central cavity. Differentiation did not occur when culture conditions did not allow the fibroblastic sheet to be established or resulted in the absence of a cavity. The importance of the explant structure in differentiation is also indicated by the location of glandular cells. In vitro they differentiate near the cavity derived from Rathke's pouch lumen while in situ they first appear at the periphery of the rudiment, near the basement membrane. The results of this study show that the adenohypophyseal primordium can differentiate without any mesenchymal influence. The observed differentiation seems to be correlated with fibroblast-secreted material, and this hypothesis is discussed."}
{"id": "PMID:475000", "title": "The development of monoaminergic brainstem-spinal systems in the North American opossum.", "content": "Evidence is presented for an early appearance of monoaminergic neurites within the spinal cord of the developing opossum. They are present within the marginal zone before hindlimb movements begin (stage I) and they start to grow into the intermediate zone by the time hindlimb movements are first observed (stage II). Monoaminergic neurites grow first into the dorsolateral intermediate zone and the intermediolateral cell column where they can be found by the beginning of stage II. Shortly thereafter, fluorescent varicosities can be traced into the area dorsal to the central canal presumed to become lamina X. Fluorescent processes extend in to the ventral intermediate zone (ventral horn) somewhat later in development. Monoaminergic axons have grown into all of the areas they occupy in the adult animal, except for laminae I and II, by the time immature hindlimb movements can be altered by cutting all brainstem projections to the lumbosacral cord (stage III). Monoaminergic innervation of laminae I and II is the last to develope, but it is present by the time thoracic transection produces complete spinal shock.", "contents": "The development of monoaminergic brainstem-spinal systems in the North American opossum. Evidence is presented for an early appearance of monoaminergic neurites within the spinal cord of the developing opossum. They are present within the marginal zone before hindlimb movements begin (stage I) and they start to grow into the intermediate zone by the time hindlimb movements are first observed (stage II). Monoaminergic neurites grow first into the dorsolateral intermediate zone and the intermediolateral cell column where they can be found by the beginning of stage II. Shortly thereafter, fluorescent varicosities can be traced into the area dorsal to the central canal presumed to become lamina X. Fluorescent processes extend in to the ventral intermediate zone (ventral horn) somewhat later in development. Monoaminergic axons have grown into all of the areas they occupy in the adult animal, except for laminae I and II, by the time immature hindlimb movements can be altered by cutting all brainstem projections to the lumbosacral cord (stage III). Monoaminergic innervation of laminae I and II is the last to develope, but it is present by the time thoracic transection produces complete spinal shock."}
{"id": "PMID:475001", "title": "A re-examination of mitotic activity in the early chick embryo.", "content": "As a result of extensive mitotic index analysis in colchicine-arrested chick embryos during gastrulation, it was ascertained that the primitive streak is a region of elevated mitotic index as compared to the surrounding tissue. Along the cephalo-caudal axis, the embryo displays two large peaks of mitotic index, one at the posterior end of the primitive streak and the other just anterior to Hensen's node. The length of the various phases of the mitotic period was determined in vitro by time-lapse filming, and the colchicine-arrested mitotic indices in vivo and in vitro were determined and compared for various regions. Some observations regarding the orientation of mitotic spindles and abnormal mitosis in vitro are also included, and the relevance of the above observations to early embryonic development is discussed.", "contents": "A re-examination of mitotic activity in the early chick embryo. As a result of extensive mitotic index analysis in colchicine-arrested chick embryos during gastrulation, it was ascertained that the primitive streak is a region of elevated mitotic index as compared to the surrounding tissue. Along the cephalo-caudal axis, the embryo displays two large peaks of mitotic index, one at the posterior end of the primitive streak and the other just anterior to Hensen's node. The length of the various phases of the mitotic period was determined in vitro by time-lapse filming, and the colchicine-arrested mitotic indices in vivo and in vitro were determined and compared for various regions. Some observations regarding the orientation of mitotic spindles and abnormal mitosis in vitro are also included, and the relevance of the above observations to early embryonic development is discussed."}
{"id": "PMID:475002", "title": "Neurogenesis in the basal forebrain of the Chinese hamster (Cricetulus griseus). I. Time of neuron origin.", "content": "The time of neuron origin has been determined in the basal ganglia and related basal forebrain structures of the Chinese hamster with the aid of 3H-thymidine autoradiography. Large-celled structures like the globus pallidus, nucleus of the horizontal limb of the diagonal band of Broca as well as large cells in the rostral part of the substantia innominata, in the caudate-putamen-complex and in the olfactory tubercle arise early (E12--E16), whereas medium-sized and small cells in the basal forebrain have a persistent origin over a much longer period. Neuron formation in the basal forebrain persists decrementally until P4. A clear caudorostral spatiotemporal gradient as well as a distinct 'outside-in' gradient have been observed in the caudate-putamen-complex. Medium-sized neurons in the neostriatum and in the nucleus accumbens, generated simultaneously, are usually arranged in scattered clusters. The present data on time of neuron origin strongly support other evidence which points to the conclusion that the nucleus accumbens can be considered as a ventromedial extension of the caudate-putamen-complex.", "contents": "Neurogenesis in the basal forebrain of the Chinese hamster (Cricetulus griseus). I. Time of neuron origin. The time of neuron origin has been determined in the basal ganglia and related basal forebrain structures of the Chinese hamster with the aid of 3H-thymidine autoradiography. Large-celled structures like the globus pallidus, nucleus of the horizontal limb of the diagonal band of Broca as well as large cells in the rostral part of the substantia innominata, in the caudate-putamen-complex and in the olfactory tubercle arise early (E12--E16), whereas medium-sized and small cells in the basal forebrain have a persistent origin over a much longer period. Neuron formation in the basal forebrain persists decrementally until P4. A clear caudorostral spatiotemporal gradient as well as a distinct 'outside-in' gradient have been observed in the caudate-putamen-complex. Medium-sized neurons in the neostriatum and in the nucleus accumbens, generated simultaneously, are usually arranged in scattered clusters. The present data on time of neuron origin strongly support other evidence which points to the conclusion that the nucleus accumbens can be considered as a ventromedial extension of the caudate-putamen-complex."}
{"id": "PMID:475003", "title": "The choroid plexus of the mature and aging rat: the choroidal epithelium.", "content": "The choroid plexus of mature and old rats has been examined by both scanning and transmission electron microscopy. It has been shown that the macrophages lying upon the ventricular surface of the choroid plexus have a close association with burr-like protrusions that extend from the apical surfaces of the choroidal epithelial cells. These protrusions have a dark cytoplasm filled with vesicles and tubules, and projecting from them are thin, shrunken microvilli. It is suggested that these protrusions are phagocytosed by the macrophages and that they are the source of some of the inclusions which become increasingly common within the cytoplasm of macrophages in older rats. The lateral surfaces of the choroidal epithelial cells have also been examined in the scanning electron microscope after exposure of the surfaces by dissection. In such preparations it is apparent that the elaborate interdigitations between adjacent cells are effected by irregular and vertically arranged folds confined to the basal portions of the lateral cell surfaces. Lastly, it has been shown that at the junction between the choroid plexus and the ependyma in the lateral ventricle, there are two modes of transition between the choroidal and ependymal epithelia. In one, typical choroidal and ependymal epithelial cells lie next to each other to produce a distinct and continuous bondary. In the other mode the boundary is also continuous, but there are modified ependymal cells present. These modified cells have short, relatively sparsely distributed microvilli and not more than one or two cilia.", "contents": "The choroid plexus of the mature and aging rat: the choroidal epithelium. The choroid plexus of mature and old rats has been examined by both scanning and transmission electron microscopy. It has been shown that the macrophages lying upon the ventricular surface of the choroid plexus have a close association with burr-like protrusions that extend from the apical surfaces of the choroidal epithelial cells. These protrusions have a dark cytoplasm filled with vesicles and tubules, and projecting from them are thin, shrunken microvilli. It is suggested that these protrusions are phagocytosed by the macrophages and that they are the source of some of the inclusions which become increasingly common within the cytoplasm of macrophages in older rats. The lateral surfaces of the choroidal epithelial cells have also been examined in the scanning electron microscope after exposure of the surfaces by dissection. In such preparations it is apparent that the elaborate interdigitations between adjacent cells are effected by irregular and vertically arranged folds confined to the basal portions of the lateral cell surfaces. Lastly, it has been shown that at the junction between the choroid plexus and the ependyma in the lateral ventricle, there are two modes of transition between the choroidal and ependymal epithelia. In one, typical choroidal and ependymal epithelial cells lie next to each other to produce a distinct and continuous bondary. In the other mode the boundary is also continuous, but there are modified ependymal cells present. These modified cells have short, relatively sparsely distributed microvilli and not more than one or two cilia."}
{"id": "PMID:475004", "title": "Mandibular growth retardation in corticosteroid-treated juvenile mice.", "content": "Juvenile mice were treated for up to eight weeks with weekly doses of a synthetic analogue of cortisol:triamcinolone hexacetonide. The mandibular condylar cartilage was studied histologically and histochemically at regular intervals. Morphometric measurements were performed along the mandibular posterior vertical dimension (condylar process and ramus). By the second injection significant morphological changes were noted in the condylar cartilage, followed by retardation of bone growth. The most distinctive feature in the cartilage of triamcinolone-treated mice was a marked increase in the dimension of its mineralized zone concomitant with a significant increase in the number of hypertrophic chondrocytes. The role of condylar cartilage in mandibular growth is discussed.", "contents": "Mandibular growth retardation in corticosteroid-treated juvenile mice. Juvenile mice were treated for up to eight weeks with weekly doses of a synthetic analogue of cortisol:triamcinolone hexacetonide. The mandibular condylar cartilage was studied histologically and histochemically at regular intervals. Morphometric measurements were performed along the mandibular posterior vertical dimension (condylar process and ramus). By the second injection significant morphological changes were noted in the condylar cartilage, followed by retardation of bone growth. The most distinctive feature in the cartilage of triamcinolone-treated mice was a marked increase in the dimension of its mineralized zone concomitant with a significant increase in the number of hypertrophic chondrocytes. The role of condylar cartilage in mandibular growth is discussed."}
{"id": "PMID:475005", "title": "Intraspinal non-neuronal cellular responses to peripheral nerve injury.", "content": "The accumulation of non-vascular, non-neuronal cells (designated herein as reactive cells) in association with perikarya of axotomized motor neurons has been described by many investigators. Recently Gilmore ('75) found that sciatic axotomy in immature rats resulted in the occurrence of reactive cells not only in the spinal ventral gray matter but also in the dorsal gray matter. To determine if the presence of these cells in the dorsal gray matter, a finding not reported by others, was related to the immaturity of the animal, sciatic axotomy was performed in rats ranging in age from 17 days to 16 months in the present study. Light microscopic evaluation of the spinal cords three or seven days post-operatively showed that the reactive cells occurred consistently in both dorsal and ventral gray matter irrespective of age. Transection of tibial nerve or the nerve to the medial head of the gastrocnemius muscle elicited a cellular response in both dorsal and ventral gray matter, although transection of the latter nerve resulted in a much less obvious response. Crushing of the sciatic nerve was followed by a response of reactive cells not qualitatively different from that noted following transection. Transection of the sural nerve, primarily a sensory nerve, resulted in the presence of reactive cells in dorsal gray matter but not in the environs of motor neurons in the ventral gray matter. These findings suggest that the reactive cells in the dorsal gray matter of the spinal cord are associated with altered central processes of dorsal root ganglion cells.", "contents": "Intraspinal non-neuronal cellular responses to peripheral nerve injury. The accumulation of non-vascular, non-neuronal cells (designated herein as reactive cells) in association with perikarya of axotomized motor neurons has been described by many investigators. Recently Gilmore ('75) found that sciatic axotomy in immature rats resulted in the occurrence of reactive cells not only in the spinal ventral gray matter but also in the dorsal gray matter. To determine if the presence of these cells in the dorsal gray matter, a finding not reported by others, was related to the immaturity of the animal, sciatic axotomy was performed in rats ranging in age from 17 days to 16 months in the present study. Light microscopic evaluation of the spinal cords three or seven days post-operatively showed that the reactive cells occurred consistently in both dorsal and ventral gray matter irrespective of age. Transection of tibial nerve or the nerve to the medial head of the gastrocnemius muscle elicited a cellular response in both dorsal and ventral gray matter, although transection of the latter nerve resulted in a much less obvious response. Crushing of the sciatic nerve was followed by a response of reactive cells not qualitatively different from that noted following transection. Transection of the sural nerve, primarily a sensory nerve, resulted in the presence of reactive cells in dorsal gray matter but not in the environs of motor neurons in the ventral gray matter. These findings suggest that the reactive cells in the dorsal gray matter of the spinal cord are associated with altered central processes of dorsal root ganglion cells."}
{"id": "PMID:475006", "title": "Effect of thyroparathyroidectomy (TPX) on the zona reticularis: a quantitative ultrastructural study.", "content": "Atrophy of zona reticularis cells was observed two weeks after surgical thyroparathyroidectomy (TPX). Quantitative morphological techniques for electron microscopy showed significant decreases in the volume of cytoplasm, nucleus, mitochondria, smooth endoplasmic reticulum and lipid droplets in the zona reticularis of TPX rats. In addition, many mitochnodria contained lipid droplets, some of which occupied virtually the entire matrix of mitochondria. The volume per cell of mitochondria with these inclusions increased significantly after TPX. The lipid droplets may well arise from cytoplasmic droplets by increased transport or reduced metabolism of cholesterol, or by direct incorporation of droplets into the mitochondrial matrix. The serum corticosterone level of TPX rats sacrificed under quiescent conditions did not differ significantly from that of controls. Hypothyroidism induced a significant increase in the volume of peroxisomes per cell in TPX animals which may be related to changes in lipid metabolism or transport.", "contents": "Effect of thyroparathyroidectomy (TPX) on the zona reticularis: a quantitative ultrastructural study. Atrophy of zona reticularis cells was observed two weeks after surgical thyroparathyroidectomy (TPX). Quantitative morphological techniques for electron microscopy showed significant decreases in the volume of cytoplasm, nucleus, mitochondria, smooth endoplasmic reticulum and lipid droplets in the zona reticularis of TPX rats. In addition, many mitochnodria contained lipid droplets, some of which occupied virtually the entire matrix of mitochondria. The volume per cell of mitochondria with these inclusions increased significantly after TPX. The lipid droplets may well arise from cytoplasmic droplets by increased transport or reduced metabolism of cholesterol, or by direct incorporation of droplets into the mitochondrial matrix. The serum corticosterone level of TPX rats sacrificed under quiescent conditions did not differ significantly from that of controls. Hypothyroidism induced a significant increase in the volume of peroxisomes per cell in TPX animals which may be related to changes in lipid metabolism or transport."}
{"id": "PMID:475007", "title": "Role of the left interventricular sulcus in formation of interventricular septum and crista supraventricularis in normal human cardiogenesis.", "content": "Serial sections of normal human embryos were studied and three-dimensional images reconstructed to determine the early development of the interventricular septum. The position of the interventricular septum is determined in stage 9 of normal development by the formation of the left interventricular sulcus. As a result of unknown properties of the cells of the myocardial layer, the left interventricular sulcus persists while the right disappears, producing the initial lateral asymmetry of the primary heart tube. By stage 14, the left interventricular sulcus forms a spiral which is continuous with the developing interventricular septum. The dorsal limb of the spiral passes to the right between the atrioventricular canal and the origin of the outflow tract, and is lost in the wall of the trabeculated right ventricle. It appears that this dorsal limb of the spiral is the precursor of part of the cirsta supraventricularis. The midportion of the sulcus, the bulboventricular groove, becomes the so-called fibrous continuity between the aortic and mitral valves. The ventral limb of the spiral passes caudally in the anterior interventricular groove and then dorsally and cranially toward the dorsal cushion of the atrioventricular canal. The ventral limb of the spiral is continuous with the crest of the muscular interventricular septum, which develops by apposition of tissue from the expanding right and left ventricles. From stage 14 to stage 19, the muscular interventricular septum, the atrioventricular endocardial cushions, and the ventricular end of the spiral ridges of the outflow tract appose and fuse. Subsequent formation of the membranous interventricular septum completes the physical separation of the right and left ventricles.", "contents": "Role of the left interventricular sulcus in formation of interventricular septum and crista supraventricularis in normal human cardiogenesis. Serial sections of normal human embryos were studied and three-dimensional images reconstructed to determine the early development of the interventricular septum. The position of the interventricular septum is determined in stage 9 of normal development by the formation of the left interventricular sulcus. As a result of unknown properties of the cells of the myocardial layer, the left interventricular sulcus persists while the right disappears, producing the initial lateral asymmetry of the primary heart tube. By stage 14, the left interventricular sulcus forms a spiral which is continuous with the developing interventricular septum. The dorsal limb of the spiral passes to the right between the atrioventricular canal and the origin of the outflow tract, and is lost in the wall of the trabeculated right ventricle. It appears that this dorsal limb of the spiral is the precursor of part of the cirsta supraventricularis. The midportion of the sulcus, the bulboventricular groove, becomes the so-called fibrous continuity between the aortic and mitral valves. The ventral limb of the spiral passes caudally in the anterior interventricular groove and then dorsally and cranially toward the dorsal cushion of the atrioventricular canal. The ventral limb of the spiral is continuous with the crest of the muscular interventricular septum, which develops by apposition of tissue from the expanding right and left ventricles. From stage 14 to stage 19, the muscular interventricular septum, the atrioventricular endocardial cushions, and the ventricular end of the spiral ridges of the outflow tract appose and fuse. Subsequent formation of the membranous interventricular septum completes the physical separation of the right and left ventricles."}
{"id": "PMID:475008", "title": "Early cytodifferentiation of human prostatic urethra and Leydig cells.", "content": "The ultrastructure of the urethral epithelium and mesenchyme of the 6- to 9-week-old human embryos was studied in order to reveal early morphological signs of prostatic development. The morphological changes of the urethral wall were correlated with the cytodifferentiation of the Leydig cells of the same embryos. Throughout the study the urethral epithelium had two or more layers of cuboidal cells. The ultrastructure of the cells was primitive and they did not achieve characteristics of the secretory prostatic cell. The surface cells had well developed apical junctions and slender cytoplasmic processes projecting into widened intercellular spaces appeared during the developmental period. The urethral mesenchyme showed the most salient changes. The mesenchymal cells adjacent to the urethral epithelium differentiated in the ninth week inot fibroblast-like cells with an elongated shape and cytoplasmic processes. Granular endoplamic reticulum appeared in the cytoplasm and collagen fibers were seen in the intercellular space. Mesenchymal cell processes contacting the continuous basal lamina under the epithelium were present. No direct epithelio-mesenchymal cellular contacts could be seen. The differentiation of the mesenchyme before the epithelial outgrowths that the mesenchyme has an essential role in the glandular development. Electron microscopic study of the Leydig cells showed that the amount of agranular endoplasmic reticulum increased considerably in the ninth week. This agrees with earlier biochemical findings on the capability of Leydig cells to produce androgens by this time. The temporal relationship between the cytodifferentiation of Leydig cells and the urethral wall is consistent with the idea that in the human, fetal androgens induce prostatic development.", "contents": "Early cytodifferentiation of human prostatic urethra and Leydig cells. The ultrastructure of the urethral epithelium and mesenchyme of the 6- to 9-week-old human embryos was studied in order to reveal early morphological signs of prostatic development. The morphological changes of the urethral wall were correlated with the cytodifferentiation of the Leydig cells of the same embryos. Throughout the study the urethral epithelium had two or more layers of cuboidal cells. The ultrastructure of the cells was primitive and they did not achieve characteristics of the secretory prostatic cell. The surface cells had well developed apical junctions and slender cytoplasmic processes projecting into widened intercellular spaces appeared during the developmental period. The urethral mesenchyme showed the most salient changes. The mesenchymal cells adjacent to the urethral epithelium differentiated in the ninth week inot fibroblast-like cells with an elongated shape and cytoplasmic processes. Granular endoplamic reticulum appeared in the cytoplasm and collagen fibers were seen in the intercellular space. Mesenchymal cell processes contacting the continuous basal lamina under the epithelium were present. No direct epithelio-mesenchymal cellular contacts could be seen. The differentiation of the mesenchyme before the epithelial outgrowths that the mesenchyme has an essential role in the glandular development. Electron microscopic study of the Leydig cells showed that the amount of agranular endoplasmic reticulum increased considerably in the ninth week. This agrees with earlier biochemical findings on the capability of Leydig cells to produce androgens by this time. The temporal relationship between the cytodifferentiation of Leydig cells and the urethral wall is consistent with the idea that in the human, fetal androgens induce prostatic development."}
{"id": "PMID:475009", "title": "The topography of rat submandibular gland parenchyma as observed with S.E.M.", "content": "The parenchymal components of rat submandibular gland were examined by scanning electron microscopy after removal of the stromal connective tissue by acid hydrolysis and/or enzymatic digestion. Secretory acini have polymorphous topographies but usually consist of three to five truncated lobes emanating from a common hilus. They have a mean volume of about 33,000 micrometers3. Myoepithelial cells are associated with the deeper acini of the gland where they cover congruent to 54% of the surface area of their allied acini. Each flattened myoepithelial cell has a central perikaryon that gives rise to four or five broad primary processes that bifurcate two or three times to produce a total complement of 20 to 40 terminal processes per cell, each 0.5 micrometer to 1.7 micrometers wide. The terminal processes of adjacent myoepithelial cells tend to slightly overlap and to attach to each other by desmosomes forming a network over adjacent acini. The components of the duct system are examined and each characterized by salient features as observed with the SEM.", "contents": "The topography of rat submandibular gland parenchyma as observed with S.E.M. The parenchymal components of rat submandibular gland were examined by scanning electron microscopy after removal of the stromal connective tissue by acid hydrolysis and/or enzymatic digestion. Secretory acini have polymorphous topographies but usually consist of three to five truncated lobes emanating from a common hilus. They have a mean volume of about 33,000 micrometers3. Myoepithelial cells are associated with the deeper acini of the gland where they cover congruent to 54% of the surface area of their allied acini. Each flattened myoepithelial cell has a central perikaryon that gives rise to four or five broad primary processes that bifurcate two or three times to produce a total complement of 20 to 40 terminal processes per cell, each 0.5 micrometer to 1.7 micrometers wide. The terminal processes of adjacent myoepithelial cells tend to slightly overlap and to attach to each other by desmosomes forming a network over adjacent acini. The components of the duct system are examined and each characterized by salient features as observed with the SEM."}
{"id": "PMID:475010", "title": "Morphological study of the desert heteromyid kidney with emphasis on the genus Perognathus.", "content": "The renal morphology of three species of desert dwelling Perognathus rodents were compared to Dipodomys and two species of sympatric cricetid rodents. Perognathus has a highly adapted unipolar kidney capable of urine concentration up to 7,500 mOsm/KgH2O. Two major modifications were observed in these kidneys. (1) There is elongation of both the inner and outer medulla. When the thickness of the regions in P. penicillatus are factored by kidney weight it is found that the outer medulla is 3.8 and the inner medulla is 2.2 times greater in length than the extensively studied Dipodomys merriami. (2) There is great variance in glomerular size with approximately 20% of the glomeruli being very large and located in the juxta medullary region. These glomeruli are 1.5--1.6 times greater in diameter than the more numerous superficial glomeruli. These structural modifications are quantitated and the functional implications are discussed particularly in relation to urine concentrating ability in these desert inhabitants.", "contents": "Morphological study of the desert heteromyid kidney with emphasis on the genus Perognathus. The renal morphology of three species of desert dwelling Perognathus rodents were compared to Dipodomys and two species of sympatric cricetid rodents. Perognathus has a highly adapted unipolar kidney capable of urine concentration up to 7,500 mOsm/KgH2O. Two major modifications were observed in these kidneys. (1) There is elongation of both the inner and outer medulla. When the thickness of the regions in P. penicillatus are factored by kidney weight it is found that the outer medulla is 3.8 and the inner medulla is 2.2 times greater in length than the extensively studied Dipodomys merriami. (2) There is great variance in glomerular size with approximately 20% of the glomeruli being very large and located in the juxta medullary region. These glomeruli are 1.5--1.6 times greater in diameter than the more numerous superficial glomeruli. These structural modifications are quantitated and the functional implications are discussed particularly in relation to urine concentrating ability in these desert inhabitants."}
{"id": "PMID:475011", "title": "Ability of neural crest cells from the embryonic chick to differentiate into cartilage before their migration away from the neural tube.", "content": "Whether neural crest cells from the avian embryo are determined for chondrogenesis before they begin their migration away from the neural tube (i.e., before H. H. stages 8.5--9) was investigated by establishing neural folds from embryos of H. H. stages 5--11 either in organ culture, or as grafts to the chorioallantoic membranes of host embryos. Cartilage differentiated from neural folds taken from embryos of H. H. stages 5--7 but not from those taken from older embryos. This stage specific pattern was reversed when the tissue adjacent to the neural tube was grafted to the chorioallantoic membrane. Cartilage only formed from tissues isolated later than H. H. stage 8; i.e., when these adjacent tissues contain neural crest cells. We concluded that neural crest cells are determined for chondrogenesis while still in the neural tube and before their migration to the face and head. This is in contrast to the situation in the only other group which has been examined, the urodele amphibians.", "contents": "Ability of neural crest cells from the embryonic chick to differentiate into cartilage before their migration away from the neural tube. Whether neural crest cells from the avian embryo are determined for chondrogenesis before they begin their migration away from the neural tube (i.e., before H. H. stages 8.5--9) was investigated by establishing neural folds from embryos of H. H. stages 5--11 either in organ culture, or as grafts to the chorioallantoic membranes of host embryos. Cartilage differentiated from neural folds taken from embryos of H. H. stages 5--7 but not from those taken from older embryos. This stage specific pattern was reversed when the tissue adjacent to the neural tube was grafted to the chorioallantoic membrane. Cartilage only formed from tissues isolated later than H. H. stage 8; i.e., when these adjacent tissues contain neural crest cells. We concluded that neural crest cells are determined for chondrogenesis while still in the neural tube and before their migration to the face and head. This is in contrast to the situation in the only other group which has been examined, the urodele amphibians."}
{"id": "PMID:475012", "title": "Mitoses in peripheral pulmonary epithelium: the effect of colchicine.", "content": "Injury in the periphery of the lung was studied in rats given intratracheal trypsin. Mitotic figures were noted in the alveolar epithelium, and colchicine enhanced the yield. Of 65 mitotic figures examined in detail, 46 were present in the alveolar epithelium. Epithelial mitoses were confined to type II cells, many of which contained lamellar inclusions, abundant rough endoplasmic reticulum, and luminal microvilli. Mitoses were not seen in type I cells or in less well differentiated epithelial cells, but they were noted in occasional endothelial and interstitial cells. Colchicine-arrested type II cells were distinguished by radially disposed chromosomes surrounding pairs of centrioles and randomly oriented microtubules. In addition, many of these cells showed a pronounced cortical zone, devoid of microvilli, and others showed cytotoxic degenerative changes. These observations indicate that the alveolar epithelium is renewed exclusively by division of differentiated type II cells.", "contents": "Mitoses in peripheral pulmonary epithelium: the effect of colchicine. Injury in the periphery of the lung was studied in rats given intratracheal trypsin. Mitotic figures were noted in the alveolar epithelium, and colchicine enhanced the yield. Of 65 mitotic figures examined in detail, 46 were present in the alveolar epithelium. Epithelial mitoses were confined to type II cells, many of which contained lamellar inclusions, abundant rough endoplasmic reticulum, and luminal microvilli. Mitoses were not seen in type I cells or in less well differentiated epithelial cells, but they were noted in occasional endothelial and interstitial cells. Colchicine-arrested type II cells were distinguished by radially disposed chromosomes surrounding pairs of centrioles and randomly oriented microtubules. In addition, many of these cells showed a pronounced cortical zone, devoid of microvilli, and others showed cytotoxic degenerative changes. These observations indicate that the alveolar epithelium is renewed exclusively by division of differentiated type II cells."}
{"id": "PMID:475013", "title": "An autoradiographic and immunocytochemical study of the neonatal rat pituitary gland.", "content": "Two-day-old female rats were injected with 5 nmole/kg of 6,7-3H-11 beta-methoxy-17-ethylestradiol (R 2858 = moxestrol) and killed one hour later. The animals were decapitated and, the pituitary glands were removed, mounted on tissue holders and frozen in liquified propane. The tissue was then processed for autoradiography according to the thaw-mount technique. At the end of the exposure time, prior to photographic development, some of the tissue was fixed in 10% formalin and then photographically developed for autoradiography. The fixed tissue was subsequently stained immunocytochemically using antibodies to luteinizing hormone or prolactin. Between 10 and 15% of the cells of the pars distalis concentrated the synthetic estrogen or its metabolite. The immunocytochemical procedure revealed that both LH-gonadotrophs and lactotrophs concentrated the steroid. These studies along with earlier studies suggest that the neonatal rat pituitary contains only a small portion of the adult complement of estrogen receptors and that these receptors are dispersed across a number of cell types.", "contents": "An autoradiographic and immunocytochemical study of the neonatal rat pituitary gland. Two-day-old female rats were injected with 5 nmole/kg of 6,7-3H-11 beta-methoxy-17-ethylestradiol (R 2858 = moxestrol) and killed one hour later. The animals were decapitated and, the pituitary glands were removed, mounted on tissue holders and frozen in liquified propane. The tissue was then processed for autoradiography according to the thaw-mount technique. At the end of the exposure time, prior to photographic development, some of the tissue was fixed in 10% formalin and then photographically developed for autoradiography. The fixed tissue was subsequently stained immunocytochemically using antibodies to luteinizing hormone or prolactin. Between 10 and 15% of the cells of the pars distalis concentrated the synthetic estrogen or its metabolite. The immunocytochemical procedure revealed that both LH-gonadotrophs and lactotrophs concentrated the steroid. These studies along with earlier studies suggest that the neonatal rat pituitary contains only a small portion of the adult complement of estrogen receptors and that these receptors are dispersed across a number of cell types."}
{"id": "PMID:475014", "title": "Surface area ratios. I. A stereological method for estimating average cell changes in membrane surface areas.", "content": "The study (i) describes a method for estimating relative changes in membrane surface areas as they occur in stereological \"average cells,\" and (ii) considers the effect of the controls on these estimates. The results indicate that changes in five membranes compartments of pancreatic exocrine cells--produced by a secretagogue (carbomylcholine chloride)--were detected similarly when related to either an average cell surface (surface area ratio method) or to an average cell volume (method of Loud, '68). Changes, however, detected with surface densities, which relate these membrane compartments to 1 cm3 of exocrine cell cytoplasm or pancreas, were notably different from the first two estimates. This inconsistency could be explained by the fact that the surface densities were influenced not only by membrane changes within the exocrine cells, but also by changes in the number of these cells filling the cm3 of reference volume. Relating the data to an average cell reference--instead of 1 cm3--improved the accuracy of the estimates for changes in membrane surface areas by as much as several fold; the choice of controls had a similar several-fold effect on the results.", "contents": "Surface area ratios. I. A stereological method for estimating average cell changes in membrane surface areas. The study (i) describes a method for estimating relative changes in membrane surface areas as they occur in stereological \"average cells,\" and (ii) considers the effect of the controls on these estimates. The results indicate that changes in five membranes compartments of pancreatic exocrine cells--produced by a secretagogue (carbomylcholine chloride)--were detected similarly when related to either an average cell surface (surface area ratio method) or to an average cell volume (method of Loud, '68). Changes, however, detected with surface densities, which relate these membrane compartments to 1 cm3 of exocrine cell cytoplasm or pancreas, were notably different from the first two estimates. This inconsistency could be explained by the fact that the surface densities were influenced not only by membrane changes within the exocrine cells, but also by changes in the number of these cells filling the cm3 of reference volume. Relating the data to an average cell reference--instead of 1 cm3--improved the accuracy of the estimates for changes in membrane surface areas by as much as several fold; the choice of controls had a similar several-fold effect on the results."}
{"id": "PMID:475015", "title": "\"Silver dust\"--a tool to study growth interrelationships between bone, periosteum and muscle.", "content": "A cylinder of gelatin containing silver spongy granules was placed in and lay within the masseter muscle, the periosteum, and the mandible, and terminated in the medial pterygoid muscle of young (3 month-old) growing miniature pigs. On the basis of an animal sacrificed one week after placement of the cylinder, it was found that the suspending gelatin was removed by cellular activity. Nine months later the remaining animals were sacrificed. Periodic X-rays were taken during the course of the experiment. After sacrifice, the mandible and associated tissues were histologically examined. The results of this study suggest that the silver granules in the muscles maintained their location during growth; the silver granules in the mandible moved forward with mandibular growth. \"Slippage\" appeared to occur external to the fibrous layer of the periosteum; the site of movement was revealed by the trail of the silver granules. The described method should prove of value in studying the growth interrelationships between bone, periosteum, and muscle.", "contents": "\"Silver dust\"--a tool to study growth interrelationships between bone, periosteum and muscle. A cylinder of gelatin containing silver spongy granules was placed in and lay within the masseter muscle, the periosteum, and the mandible, and terminated in the medial pterygoid muscle of young (3 month-old) growing miniature pigs. On the basis of an animal sacrificed one week after placement of the cylinder, it was found that the suspending gelatin was removed by cellular activity. Nine months later the remaining animals were sacrificed. Periodic X-rays were taken during the course of the experiment. After sacrifice, the mandible and associated tissues were histologically examined. The results of this study suggest that the silver granules in the muscles maintained their location during growth; the silver granules in the mandible moved forward with mandibular growth. \"Slippage\" appeared to occur external to the fibrous layer of the periosteum; the site of movement was revealed by the trail of the silver granules. The described method should prove of value in studying the growth interrelationships between bone, periosteum, and muscle."}
{"id": "PMID:475016", "title": "Chromatin substructure: an electron microscopic study of thin-sectioned chromatin subjected to sequential protein extraction and water swelling procedures.", "content": "Electron microscopic observations and measurements were made on thin-sectioned chromatin fibers and fibrils obtained from nuclei of mature chicken erythrocytes. The nuclei were isolated in low ionic strength gum arabic and octanol then extracted sequentially with (1) 0.14 M NaCl, (2) 0.25 N HCl, (3) buffer saturated phenol, (4) hot 5% SDS and 0.14 M 2-mercaptoethanol and, (5) 0.4 N NaOH. The amount of nuclear protein removed at each of the first four extraction steps was 1, 86, 3 and 11% of the total, respectively. Each extract was characterized by electrophoretic profiles. At each extraction the chromatin was fixed by adding large quantities of a mixture of equal volumes of sodium cacodylate buffered 8% (w/v) glutaraldehyde (pH 6.8) and 2% OsO4 (w/v), directly into (1) an aliquot of the chromatin in extraction fluid, and (2) an aliquot of the chromatin after water washing and swelling. Three size classes of chromatin structure were seen in thin sections prepared for high resolution transmission electron microscopy and stained with uranyl acetate and lead citrate. A thick fiber of about 25 + nm diameter was the predominant large fiber seen in freshly isolated nuclei or in nuclei after salt extraction. This 25 + nm fiber has a substructure consisting of 3.2-5.2 nm diameter fibrils. After water swelling of such freshly isolated or salt extracted nuclei a fiber of about 10 nm diameter was the predominant large fiber instead of the 25 nm diameter fiber. The HCl extraction step which is known to remove histones, caused the disappearance of both the 25 nm and the 10 nm fibers. High magnification (600,000 x) micrographs of the chromatin at all procedural steps, except the last NaOH step, reveal the fibril to be omnipresent. This fibril tends to decrease somewhat in diameter during the protein extraction steps to a 2.5 nm diameter fibril after the hot SDS extraction. A fibril of 2.5 nm diameter is expected of naked double helical DNA stained with a positive stain. The NaOH, which is known to denature DNA, completely destroyed the remaining fibril. We inerpret our results to indicate that the larger chromatin fiber seen in micrographs of thin-sectioned chromatin has a fibrillar substructure which probably represents a double coil of native DNA which may have a thin protein coating of its own. The latter fibril may in turn be wrapped around a hydrophobic histone domain, perhaps reflected in the 10 nm diameter fiber which is seen upon swelling of the chromatin. This 10 nm diameter fiber is thought to be further packaged by folding into the 25 + nm diameter chromatin fiber most frequently reported in thin sections of eukaryotic cell nuclei in situ.", "contents": "Chromatin substructure: an electron microscopic study of thin-sectioned chromatin subjected to sequential protein extraction and water swelling procedures. Electron microscopic observations and measurements were made on thin-sectioned chromatin fibers and fibrils obtained from nuclei of mature chicken erythrocytes. The nuclei were isolated in low ionic strength gum arabic and octanol then extracted sequentially with (1) 0.14 M NaCl, (2) 0.25 N HCl, (3) buffer saturated phenol, (4) hot 5% SDS and 0.14 M 2-mercaptoethanol and, (5) 0.4 N NaOH. The amount of nuclear protein removed at each of the first four extraction steps was 1, 86, 3 and 11% of the total, respectively. Each extract was characterized by electrophoretic profiles. At each extraction the chromatin was fixed by adding large quantities of a mixture of equal volumes of sodium cacodylate buffered 8% (w/v) glutaraldehyde (pH 6.8) and 2% OsO4 (w/v), directly into (1) an aliquot of the chromatin in extraction fluid, and (2) an aliquot of the chromatin after water washing and swelling. Three size classes of chromatin structure were seen in thin sections prepared for high resolution transmission electron microscopy and stained with uranyl acetate and lead citrate. A thick fiber of about 25 + nm diameter was the predominant large fiber seen in freshly isolated nuclei or in nuclei after salt extraction. This 25 + nm fiber has a substructure consisting of 3.2-5.2 nm diameter fibrils. After water swelling of such freshly isolated or salt extracted nuclei a fiber of about 10 nm diameter was the predominant large fiber instead of the 25 nm diameter fiber. The HCl extraction step which is known to remove histones, caused the disappearance of both the 25 nm and the 10 nm fibers. High magnification (600,000 x) micrographs of the chromatin at all procedural steps, except the last NaOH step, reveal the fibril to be omnipresent. This fibril tends to decrease somewhat in diameter during the protein extraction steps to a 2.5 nm diameter fibril after the hot SDS extraction. A fibril of 2.5 nm diameter is expected of naked double helical DNA stained with a positive stain. The NaOH, which is known to denature DNA, completely destroyed the remaining fibril. We inerpret our results to indicate that the larger chromatin fiber seen in micrographs of thin-sectioned chromatin has a fibrillar substructure which probably represents a double coil of native DNA which may have a thin protein coating of its own. The latter fibril may in turn be wrapped around a hydrophobic histone domain, perhaps reflected in the 10 nm diameter fiber which is seen upon swelling of the chromatin. This 10 nm diameter fiber is thought to be further packaged by folding into the 25 + nm diameter chromatin fiber most frequently reported in thin sections of eukaryotic cell nuclei in situ."}
{"id": "PMID:475017", "title": "Ultrastructure of the hepatic perisinusoidal cells in man and other mammalian species.", "content": "Perisinusoidal (P.S.) cells occurring in the spaces of Disse in the livers of normal cats, dogs, miniature pigs, albino rats, human adults and children were examined by electron microscopy. The ultrastructural details of the P.S. cells and their topographic relationships with hepatocytes, sinusoidal lining cells and reticulum fibers are described. Species differences between P.S. cells were primarily a dissimilarity in lipid content: the main ultrastructural features were the same in all species studied. The P.S. cells of the rat liver displayed only low endocytotic activity, and no phagosome formation following intravenous administration of horseradish peroxidase. The close topographic relationship of the P.S. cells with the intralobular reticulum fibers was reminiscent of the intimate connection between fibroblasts and collagen fibers, or, in cat liver, of the reticulum cell--reticulum fiber association seen in lymphoid organs. Fibroblasts were not found inside the hepatic lobules. These findings support the conclusion that the reticulum fibers of hepatic lobules are produced by perisinusoidal cells which, however, display also other functions.", "contents": "Ultrastructure of the hepatic perisinusoidal cells in man and other mammalian species. Perisinusoidal (P.S.) cells occurring in the spaces of Disse in the livers of normal cats, dogs, miniature pigs, albino rats, human adults and children were examined by electron microscopy. The ultrastructural details of the P.S. cells and their topographic relationships with hepatocytes, sinusoidal lining cells and reticulum fibers are described. Species differences between P.S. cells were primarily a dissimilarity in lipid content: the main ultrastructural features were the same in all species studied. The P.S. cells of the rat liver displayed only low endocytotic activity, and no phagosome formation following intravenous administration of horseradish peroxidase. The close topographic relationship of the P.S. cells with the intralobular reticulum fibers was reminiscent of the intimate connection between fibroblasts and collagen fibers, or, in cat liver, of the reticulum cell--reticulum fiber association seen in lymphoid organs. Fibroblasts were not found inside the hepatic lobules. These findings support the conclusion that the reticulum fibers of hepatic lobules are produced by perisinusoidal cells which, however, display also other functions."}
{"id": "PMID:475018", "title": "Response of periodontal ligament cells to orthodontic force: ultrastructural identification of proliferating fibroblasts.", "content": "The morphologic response of periodontal ligament (PDL) cells in an area of tension created by orthodontic force has been assessed by transmission electron microscopy. Young adult male rats were sacrificed at 24, 48, 72, 96 and 120 hours following orthodontic stimulation. The earliest detectable response was the appearance of increased numbers of mitotic cells in the PDL at 24 hours post-stimulation. The most significant ultrastructural feature of these cells was the presence of intracellular vesicles containing collagen microfibrils. These vesicles were identical to profiles present in interphase PDL fibroblasts involved in collagen phagocytosis associated with turnover of the ligament. Between 48 and 120 hours the alveolar bone surface in the region examined was characterized by the presence of newly generated osteoblasts and active bone formation. Intracellular collagen was never observed in osteoblasts. These observations suggest that at least a portio- of the population of PDL cells which proliferate in response to orthodontic force represent functional ligament fibroblasts.", "contents": "Response of periodontal ligament cells to orthodontic force: ultrastructural identification of proliferating fibroblasts. The morphologic response of periodontal ligament (PDL) cells in an area of tension created by orthodontic force has been assessed by transmission electron microscopy. Young adult male rats were sacrificed at 24, 48, 72, 96 and 120 hours following orthodontic stimulation. The earliest detectable response was the appearance of increased numbers of mitotic cells in the PDL at 24 hours post-stimulation. The most significant ultrastructural feature of these cells was the presence of intracellular vesicles containing collagen microfibrils. These vesicles were identical to profiles present in interphase PDL fibroblasts involved in collagen phagocytosis associated with turnover of the ligament. Between 48 and 120 hours the alveolar bone surface in the region examined was characterized by the presence of newly generated osteoblasts and active bone formation. Intracellular collagen was never observed in osteoblasts. These observations suggest that at least a portio- of the population of PDL cells which proliferate in response to orthodontic force represent functional ligament fibroblasts."}
{"id": "PMID:475020", "title": "Mortality experience among anesthesiologists, 1954-1976.", "content": "To determine whether anesthesiologists, because of prolonged exposure to halothane or other inhalational anesthetics, might have higher death rates from all causes, from cancer, and from hepatic or renal disease than other physicians, the mortality rates of male and female members of the American Society of Anesthesiologists (ASA) for the period 1954 to 1976 were examined. Records of all physicians on ASA membership lists in 1954, 1959, 1967 and 1976 were obtained. All members, including life members, retired members and those who had stopped paying dues, were traced. Of the 637 deaths between 1954 and 1976 in male anesthesiologists, death certificates were obtained for 610. Mortality from all causes in those members who were accredited prior to 1960 was 84% of the expected death rate of all physicians. Among male members of record in 1967, it was 93% of the expected rate. There was no suggestion of an increased rate of cancer or hepatic or renal disease. There was a high suicide rate in members less than 55 years old. The overall mortality rate for the relatively small group (n = 66) of female anesthesiologists was 75 to 85% of that expected for all female physicians.", "contents": "Mortality experience among anesthesiologists, 1954-1976. To determine whether anesthesiologists, because of prolonged exposure to halothane or other inhalational anesthetics, might have higher death rates from all causes, from cancer, and from hepatic or renal disease than other physicians, the mortality rates of male and female members of the American Society of Anesthesiologists (ASA) for the period 1954 to 1976 were examined. Records of all physicians on ASA membership lists in 1954, 1959, 1967 and 1976 were obtained. All members, including life members, retired members and those who had stopped paying dues, were traced. Of the 637 deaths between 1954 and 1976 in male anesthesiologists, death certificates were obtained for 610. Mortality from all causes in those members who were accredited prior to 1960 was 84% of the expected death rate of all physicians. Among male members of record in 1967, it was 93% of the expected rate. There was no suggestion of an increased rate of cancer or hepatic or renal disease. There was a high suicide rate in members less than 55 years old. The overall mortality rate for the relatively small group (n = 66) of female anesthesiologists was 75 to 85% of that expected for all female physicians."}
{"id": "PMID:475021", "title": "Myocardial metabolism and oxygenation in man awake and during halothane anesthesia.", "content": "Cardiac catheters were placed in seven healthy conscious patients so that aortic and left ventricular pressures (and the derivative), cardiac output (thermodilution) and myocardial blood flow (argon washin) could be measured. Blood was drawn for measurement of arterial blood-gas and arterial and coronary venous oxygen, glucose, lactate, pyruvate and fatty acid values. After induction of anesthesia by inhalation of halothane, the measurements were made during administration of low (0.70%) and high (1.54%) end-tidal halothane concentrations. Myocardial function decreased in a dose-related fashion without a change in heart rate. Myocardial blood flow and oxygen consumption were depressed in a similar manner. Myocardial oxygen extraction decreased and lactate did not change, suggesting that myocardial oxygenation was adequate. The heart rate-systolic blood pressure product correlated poorly with myocardial oxygen consumption. Systolic blood and the contractile performance index dP/dt/IP were better correlated with myocardial oxygen consumption, but the value of the coefficient was still low. Without significant changes in heart rate, systolic blood pressure is the best correlate of myocardial oxygen consumption in healthy man during the myocardial depression produced by halothane.", "contents": "Myocardial metabolism and oxygenation in man awake and during halothane anesthesia. Cardiac catheters were placed in seven healthy conscious patients so that aortic and left ventricular pressures (and the derivative), cardiac output (thermodilution) and myocardial blood flow (argon washin) could be measured. Blood was drawn for measurement of arterial blood-gas and arterial and coronary venous oxygen, glucose, lactate, pyruvate and fatty acid values. After induction of anesthesia by inhalation of halothane, the measurements were made during administration of low (0.70%) and high (1.54%) end-tidal halothane concentrations. Myocardial function decreased in a dose-related fashion without a change in heart rate. Myocardial blood flow and oxygen consumption were depressed in a similar manner. Myocardial oxygen extraction decreased and lactate did not change, suggesting that myocardial oxygenation was adequate. The heart rate-systolic blood pressure product correlated poorly with myocardial oxygen consumption. Systolic blood and the contractile performance index dP/dt/IP were better correlated with myocardial oxygen consumption, but the value of the coefficient was still low. Without significant changes in heart rate, systolic blood pressure is the best correlate of myocardial oxygen consumption in healthy man during the myocardial depression produced by halothane."}
{"id": "PMID:475062", "title": "Modification of induced airway constriction in healthy subjects.", "content": "Unlike asthmatics, healthy persons are relatively unresponsive to airway constrictors. By using partial expiratory flow-volume (PEFV) curves the authors have demontrated, in healthy subjects, dose-related decreases in flow rates following challenge with histamine and methacholine aerosols. With the use of semilogarithmic dose-response curves they have shown that 80 mg oral propranolol augmented, whereas oral pretreatment with 400 mg W10294A (an experimental bronchodilator) decreased, histamine and methacholine-induced airway constriction. However, 300 mg of orally administered aminophylline failed to modify the airway constrictor effects of histamine and methacholine. Moreover, variation was noted in induced airway constriction in healthy subjects with decreases in flow rates being greater in the morning than in the afternoon. The authors conclude that (1) propranolol augments whereas theophylline fails to prevent nonspecific induced airway constriction in healthy subjects. This suggests that the bronchodilator action of theophylline may be unrelated to its phosphodiesterase inhibitory activity, (2) control of volume history, by use of partial expiratory flow-volume curves, is useful in quantifying pharmacologic protection and augmentation of nonspecific induced airway constriction in healthy subjects and (3) conclusions resulting from observations of induced airway constriction in healthy subjects may be dependent upon the effects of diurnal variation.", "contents": "Modification of induced airway constriction in healthy subjects. Unlike asthmatics, healthy persons are relatively unresponsive to airway constrictors. By using partial expiratory flow-volume (PEFV) curves the authors have demontrated, in healthy subjects, dose-related decreases in flow rates following challenge with histamine and methacholine aerosols. With the use of semilogarithmic dose-response curves they have shown that 80 mg oral propranolol augmented, whereas oral pretreatment with 400 mg W10294A (an experimental bronchodilator) decreased, histamine and methacholine-induced airway constriction. However, 300 mg of orally administered aminophylline failed to modify the airway constrictor effects of histamine and methacholine. Moreover, variation was noted in induced airway constriction in healthy subjects with decreases in flow rates being greater in the morning than in the afternoon. The authors conclude that (1) propranolol augments whereas theophylline fails to prevent nonspecific induced airway constriction in healthy subjects. This suggests that the bronchodilator action of theophylline may be unrelated to its phosphodiesterase inhibitory activity, (2) control of volume history, by use of partial expiratory flow-volume curves, is useful in quantifying pharmacologic protection and augmentation of nonspecific induced airway constriction in healthy subjects and (3) conclusions resulting from observations of induced airway constriction in healthy subjects may be dependent upon the effects of diurnal variation."}
{"id": "PMID:475063", "title": "Neonatal IgE values in the black population.", "content": "Ninety-three cord blood samples from black neonates were evaluted for the total IgE level using the PRIST method. The results reveal values comparable to the IgE levels in other racial groups, suggesting that the influence of racial differences was not obvious at this stage.", "contents": "Neonatal IgE values in the black population. Ninety-three cord blood samples from black neonates were evaluted for the total IgE level using the PRIST method. The results reveal values comparable to the IgE levels in other racial groups, suggesting that the influence of racial differences was not obvious at this stage."}
{"id": "PMID:475064", "title": "A prospective classification of the respiratory manifestations of pollen sensitivity.", "content": "Twenty pollen sensitive subjects were classified historically as Groups I-III based upon the elicitation of lower respiratory symptoms at (I) no time of the year, (II) only during the pollen season or (III) during and apart from the pollen season respectively. Neither antigen skin sensitivity nor antigen or mecholyl inhalational sensitivity was useful in delineating these three groups. However, pulmonary function tests during the season demonstrated changes in MMEFR and airflow at 25% vital capacity in Group II subjects, confirming our classifications.", "contents": "A prospective classification of the respiratory manifestations of pollen sensitivity. Twenty pollen sensitive subjects were classified historically as Groups I-III based upon the elicitation of lower respiratory symptoms at (I) no time of the year, (II) only during the pollen season or (III) during and apart from the pollen season respectively. Neither antigen skin sensitivity nor antigen or mecholyl inhalational sensitivity was useful in delineating these three groups. However, pulmonary function tests during the season demonstrated changes in MMEFR and airflow at 25% vital capacity in Group II subjects, confirming our classifications."}
{"id": "PMID:475065", "title": "Aerosol bronchodilator therapy: a comparison of the effects of bronkometer with isoetharine, isoproterenol and phenylephrine.", "content": "The bronchodilator action and cardiovascular toxicity of aerosols of (1) isoetharine, (2) isoetharine in combination with phenylephrine, (3) isoproterenol, and (4) phenylephrine were compared in a group of severe stable ambulatory asthmatics. All preparations except phenylephrine produced reduction in specific airway resistance and increased flow rates which peaked at 15 minutes. The action of isoproterenol and the two isoetharine preparations peaked at 15 minutes while the peak effect of isoetharine and isoetharine in combination with phenylephrine continued for 60 minutes. The difference between isoproterenol and isoetharine alone or in combination with phenylephrine was not statistically significant. None of the drugs produced any cardiovascular side effects. The authors conclude that isoetharine, though less potent than isoproterenol on a weight-for-weight basis, is an effective and safe bronchodilator. Addition of phenylephrine to isoetharine does not potentiate or prolong the action of the latter.", "contents": "Aerosol bronchodilator therapy: a comparison of the effects of bronkometer with isoetharine, isoproterenol and phenylephrine. The bronchodilator action and cardiovascular toxicity of aerosols of (1) isoetharine, (2) isoetharine in combination with phenylephrine, (3) isoproterenol, and (4) phenylephrine were compared in a group of severe stable ambulatory asthmatics. All preparations except phenylephrine produced reduction in specific airway resistance and increased flow rates which peaked at 15 minutes. The action of isoproterenol and the two isoetharine preparations peaked at 15 minutes while the peak effect of isoetharine and isoetharine in combination with phenylephrine continued for 60 minutes. The difference between isoproterenol and isoetharine alone or in combination with phenylephrine was not statistically significant. None of the drugs produced any cardiovascular side effects. The authors conclude that isoetharine, though less potent than isoproterenol on a weight-for-weight basis, is an effective and safe bronchodilator. Addition of phenylephrine to isoetharine does not potentiate or prolong the action of the latter."}
{"id": "PMID:475066", "title": "A comparison of cancer occurrence in allergic and nonallergic populations.", "content": "Patients with significant allergy were surveyed with regard to the incidence of malignancy and a control group of nonallergic individuals was similarly reviewed. In the allergic population of 500 persons in the cancer-bearing age group, there were 12 malignancies, while the nonallergic group of 421 individuals had 14 malignancies. The Chi-squared test of these data gives a value of 0.75, which indicates that there is no significant difference in the incidence of malignancy in the allergic and nonallergic groups. As one would expect, the test of independence between age, cancer and allergy using the Chi-squared statistic is rejected.", "contents": "A comparison of cancer occurrence in allergic and nonallergic populations. Patients with significant allergy were surveyed with regard to the incidence of malignancy and a control group of nonallergic individuals was similarly reviewed. In the allergic population of 500 persons in the cancer-bearing age group, there were 12 malignancies, while the nonallergic group of 421 individuals had 14 malignancies. The Chi-squared test of these data gives a value of 0.75, which indicates that there is no significant difference in the incidence of malignancy in the allergic and nonallergic groups. As one would expect, the test of independence between age, cancer and allergy using the Chi-squared statistic is rejected."}
{"id": "PMID:475069", "title": "Dinitrochlorobenzene sensitization in children with bronchial asthma.", "content": "Twenty-one patients with bronchial asthma were tested by dinitrochlorobenzene. Eleven did not react (52%). Five of the patients had been previously BCG vaccinated but did not react to PPD. The results indicated that some impairment of cell-mediated immunity in asthmatics exists. The correlation between this finding and pathogenesis of asthma is discussed.", "contents": "Dinitrochlorobenzene sensitization in children with bronchial asthma. Twenty-one patients with bronchial asthma were tested by dinitrochlorobenzene. Eleven did not react (52%). Five of the patients had been previously BCG vaccinated but did not react to PPD. The results indicated that some impairment of cell-mediated immunity in asthmatics exists. The correlation between this finding and pathogenesis of asthma is discussed."}
{"id": "PMID:475070", "title": "[Diagnosis of type III hyperlipoproteinemia: contribution of an air-driven ultracentrifuge associated with an original tube-slicer (author's transl].", "content": "Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three hours (Airfuge Beckman instruments). An original tube-slicer allows the separation of the d less than 1.006 lipoproteins located into top fractions. This simple micromethod might confirm diagnosis of type III in patients and would be useful for clinical laboratories.", "contents": "[Diagnosis of type III hyperlipoproteinemia: contribution of an air-driven ultracentrifuge associated with an original tube-slicer (author's transl]. Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three hours (Airfuge Beckman instruments). An original tube-slicer allows the separation of the d less than 1.006 lipoproteins located into top fractions. This simple micromethod might confirm diagnosis of type III in patients and would be useful for clinical laboratories."}
{"id": "PMID:475071", "title": "[Application of adsorption chromatography to the colorimetric determination of urinary estrogens in late pregnancy (author's transl)].", "content": "A new extraction procedure of urinary estrogens, based upon adsorption chromatography on Amberlite XAD-2, was deviced for a selective and complete extraction of these components. Estrogens were then quantified, without preliminar hydrolysis, by the colorimetric Kober reaction, modified by Ittrich. The reliability of this method has been established by comparison with four other methods.", "contents": "[Application of adsorption chromatography to the colorimetric determination of urinary estrogens in late pregnancy (author's transl)]. A new extraction procedure of urinary estrogens, based upon adsorption chromatography on Amberlite XAD-2, was deviced for a selective and complete extraction of these components. Estrogens were then quantified, without preliminar hydrolysis, by the colorimetric Kober reaction, modified by Ittrich. The reliability of this method has been established by comparison with four other methods."}
{"id": "PMID:475077", "title": "[Detection of rubella specific IgM on gel filtration through Sephadex G 200: use of dithiothreitol and limits with MnCl2-heparine pretreatment (author's transl)].", "content": "The detection of rubella haemagglutination inhibiting antibody, in the IgM fraction of the serum, on gel filtration through Sephadex G 200, needs precautions to exclude false results. Treatment with dithiothreitol is a satisfactory method for confirming the content of rubella IgM antibody. The failure of MnCl2-heparin pretreatment to remove non specific inhibitors of rubella hemagglutinin is unfrequent (7/108) and so do be repeated. Rarely (1/108) aggregated IgG fractionates with IgM and yield false positive results.", "contents": "[Detection of rubella specific IgM on gel filtration through Sephadex G 200: use of dithiothreitol and limits with MnCl2-heparine pretreatment (author's transl)]. The detection of rubella haemagglutination inhibiting antibody, in the IgM fraction of the serum, on gel filtration through Sephadex G 200, needs precautions to exclude false results. Treatment with dithiothreitol is a satisfactory method for confirming the content of rubella IgM antibody. The failure of MnCl2-heparin pretreatment to remove non specific inhibitors of rubella hemagglutinin is unfrequent (7/108) and so do be repeated. Rarely (1/108) aggregated IgG fractionates with IgM and yield false positive results."}
{"id": "PMID:475078", "title": "[Determination of alphaxalone in serum and urine by gas-liquid chromatography. Application in anaesthesiological pharmacology (author's transl)].", "content": "The authors propose a simple and reliable method for determination of alphaxalone [3 alpha-hydroxy(5 alpha)pregnane 11,20 dione] in serum and urine, during long-duration anaesthesia using Althesin, mixture of alphaxalone and alphadolone acetate [21 acetoxy, 3 alpha-hydroxy(5 alpha)pregnane 11,20 dione]. If assays of alphadolone appear difficult and without any precision, because of numerous interferences, the determination of alphaxalone in serum and urine seems interesting for pharmacokinetic and metabolic investigation. We confirm the quick rate of disappearance of alphaxalone from blood, associated with a very active metabolism in the liver. The results show that it exists a relation between the concentration of alphaxalone in blood and the value of anaesthesia judged on clinical and electrical criteria. We cannot however conclude that Althesin presents a cumulative effect, because we used important amounts of anaesthetic agent.", "contents": "[Determination of alphaxalone in serum and urine by gas-liquid chromatography. Application in anaesthesiological pharmacology (author's transl)]. The authors propose a simple and reliable method for determination of alphaxalone [3 alpha-hydroxy(5 alpha)pregnane 11,20 dione] in serum and urine, during long-duration anaesthesia using Althesin, mixture of alphaxalone and alphadolone acetate [21 acetoxy, 3 alpha-hydroxy(5 alpha)pregnane 11,20 dione]. If assays of alphadolone appear difficult and without any precision, because of numerous interferences, the determination of alphaxalone in serum and urine seems interesting for pharmacokinetic and metabolic investigation. We confirm the quick rate of disappearance of alphaxalone from blood, associated with a very active metabolism in the liver. The results show that it exists a relation between the concentration of alphaxalone in blood and the value of anaesthesia judged on clinical and electrical criteria. We cannot however conclude that Althesin presents a cumulative effect, because we used important amounts of anaesthetic agent."}
{"id": "PMID:475079", "title": "[An automated spectrofluorometry method for urinary estrogens determination in pregnant and non-pregnant subjects (author's transl)].", "content": "An automated method has been developed for the measurement of estrogens in the urine of pregnant and non-pregnant subjects. Using the Kober-Ittrich fluorescence procedure, this automated method was applied to the assay of estrogens in the purified urinary extracts of non-pregnant women, men or children. For pregnant-women it had been possible to simplify the purification of urinary extracts, and the reaction was performed directly on the sodium hydroxide fraction. The precision, accuracy, and sensitivity of the method had been analysed.", "contents": "[An automated spectrofluorometry method for urinary estrogens determination in pregnant and non-pregnant subjects (author's transl)]. An automated method has been developed for the measurement of estrogens in the urine of pregnant and non-pregnant subjects. Using the Kober-Ittrich fluorescence procedure, this automated method was applied to the assay of estrogens in the purified urinary extracts of non-pregnant women, men or children. For pregnant-women it had been possible to simplify the purification of urinary extracts, and the reaction was performed directly on the sodium hydroxide fraction. The precision, accuracy, and sensitivity of the method had been analysed."}
{"id": "PMID:475080", "title": "[Erythrocyte bilirubin determination (author's transl)].", "content": "The erythrocyte bilirubin value is of great interest in neonate pathology. Its sudden rising reflects the overbinding capacity to plasmatic albumin. A colorimetric, simple and accurate micromethod with 2-hydrazino-benzothiazol as reagent leads to its quantitative determination. Hemolysis interference is removed. The authors have determined a mean value of erythrocyte bilirubin/total plasmatic bilirubin ratio for newborn population without clinical or biological risks of kernicterus.", "contents": "[Erythrocyte bilirubin determination (author's transl)]. The erythrocyte bilirubin value is of great interest in neonate pathology. Its sudden rising reflects the overbinding capacity to plasmatic albumin. A colorimetric, simple and accurate micromethod with 2-hydrazino-benzothiazol as reagent leads to its quantitative determination. Hemolysis interference is removed. The authors have determined a mean value of erythrocyte bilirubin/total plasmatic bilirubin ratio for newborn population without clinical or biological risks of kernicterus."}
{"id": "PMID:475083", "title": "Percutaneous recording of evoked spinal cord potentials of dogs.", "content": "Using a signal-averaging system, potentials evoked by stimulation of nerves in the limbs or tail of dogs were recorded through needle electrodes placed adjacent to the vertebral column. Cord dorsum potentials (consisting of a small triphasic wave followed by a large negative peak and a subsequent slow positive phase) were recorded at the level of the lumbar enlargement after stimulating nerves in the pelvic limb or tail, and at the cervical enlargement after forelimb nerve stimulation. Ascending volleys were recorded from appropriate levels of the spinal cord or from regions of the cauda equina. After forelimb nerve stimulation, a large slow potential, reflecting activation of an unidentified neuronal pool, was recorded at the level of the first cervical vertebra. Percutaneously recorded potentials were closely similar to those recorded directly from the dura mater, suggesting that the method accurately recorded spinal cord electrical events and, therefore, might be of use in studying clinical or experimental spinal cord disease.", "contents": "Percutaneous recording of evoked spinal cord potentials of dogs. Using a signal-averaging system, potentials evoked by stimulation of nerves in the limbs or tail of dogs were recorded through needle electrodes placed adjacent to the vertebral column. Cord dorsum potentials (consisting of a small triphasic wave followed by a large negative peak and a subsequent slow positive phase) were recorded at the level of the lumbar enlargement after stimulating nerves in the pelvic limb or tail, and at the cervical enlargement after forelimb nerve stimulation. Ascending volleys were recorded from appropriate levels of the spinal cord or from regions of the cauda equina. After forelimb nerve stimulation, a large slow potential, reflecting activation of an unidentified neuronal pool, was recorded at the level of the first cervical vertebra. Percutaneously recorded potentials were closely similar to those recorded directly from the dura mater, suggesting that the method accurately recorded spinal cord electrical events and, therefore, might be of use in studying clinical or experimental spinal cord disease."}
{"id": "PMID:475084", "title": "Ocular hypotensive effects of carbonic anhydrase inhibitors in normotensive and glaucomatous Beagles.", "content": "Four carbonic anhydrase inhibitors (acetazolamide, dichlorphenamide, ethoxzolamide, and methazolamide) cause ocular hypotony in normotensive and glaucomatous Beagles. Four dosages of acetazolamide and methazolamide and three dosages of dichlorphenamide and ethoxzolamide were evaluated. The extent of ocular hypotony after these carbonic anhydrase inhibitors was usually greater in glaucomatous Beagles than it was in normotensive Beagles.", "contents": "Ocular hypotensive effects of carbonic anhydrase inhibitors in normotensive and glaucomatous Beagles. Four carbonic anhydrase inhibitors (acetazolamide, dichlorphenamide, ethoxzolamide, and methazolamide) cause ocular hypotony in normotensive and glaucomatous Beagles. Four dosages of acetazolamide and methazolamide and three dosages of dichlorphenamide and ethoxzolamide were evaluated. The extent of ocular hypotony after these carbonic anhydrase inhibitors was usually greater in glaucomatous Beagles than it was in normotensive Beagles."}
{"id": "PMID:475085", "title": "Abnormal canine bone development associated with hypergravity exposure.", "content": "Chronic centrifugation of 85- to 92-day-old Beagles at 2.0 X g and 2.6 X g for 26 weeks during the time of active skeletal growth caused skeletal abnormalities in the radius and the ulna of ten of 11 dogs. The pattern of change mimicked that found in naturally occurring and experimentally induced premature distal ulnar physeal closure or delayed growth at this physis. Minimal changes in bone density were detected by sensitive photon absorptiometric techniques. Skeletal abnormalities also were found in five of the six cage-control dogs, although the run-control dogs were radiographically normal.", "contents": "Abnormal canine bone development associated with hypergravity exposure. Chronic centrifugation of 85- to 92-day-old Beagles at 2.0 X g and 2.6 X g for 26 weeks during the time of active skeletal growth caused skeletal abnormalities in the radius and the ulna of ten of 11 dogs. The pattern of change mimicked that found in naturally occurring and experimentally induced premature distal ulnar physeal closure or delayed growth at this physis. Minimal changes in bone density were detected by sensitive photon absorptiometric techniques. Skeletal abnormalities also were found in five of the six cage-control dogs, although the run-control dogs were radiographically normal."}
{"id": "PMID:475086", "title": "Asynchronous growth of the canine radius and ulna: effects of retardation of longitudinal growth of the radius.", "content": "After 2,000 rads of x-radiation to the right distal radial physis of eight 70-day-old dogs, the longitudinal growth of the right radius was retarded 42.1% when compared with the growth of the nonirradiated left radius. Asynchronous growth of the right radius and ulna resulted. The gross and radiographic changes that occurred over the subsequent 75 days were described. Subluxation of the humeroradial and humeroulnar joints occurred and was first observed 31 days after irradiation. By 75 days after irradiation, the radius was puller 7.8 mm distally by the radioulnar ligament. However, subluxation of the humeroradial joint was minimized by accelerated growth from the proximal radial physis and articular cartilage. Acceleration of growth also occurred at the distal radial articular cartilage. Growth from the distal ulnar physis and styloid process was retarded. It was thus apparent that after retardation of growth from the distal radial physis, considerable adjustment occurred in the relationship between the radius and the ulna and also in growth from their other physes, which reduced the deleterious effects of the growth retardation.", "contents": "Asynchronous growth of the canine radius and ulna: effects of retardation of longitudinal growth of the radius. After 2,000 rads of x-radiation to the right distal radial physis of eight 70-day-old dogs, the longitudinal growth of the right radius was retarded 42.1% when compared with the growth of the nonirradiated left radius. Asynchronous growth of the right radius and ulna resulted. The gross and radiographic changes that occurred over the subsequent 75 days were described. Subluxation of the humeroradial and humeroulnar joints occurred and was first observed 31 days after irradiation. By 75 days after irradiation, the radius was puller 7.8 mm distally by the radioulnar ligament. However, subluxation of the humeroradial joint was minimized by accelerated growth from the proximal radial physis and articular cartilage. Acceleration of growth also occurred at the distal radial articular cartilage. Growth from the distal ulnar physis and styloid process was retarded. It was thus apparent that after retardation of growth from the distal radial physis, considerable adjustment occurred in the relationship between the radius and the ulna and also in growth from their other physes, which reduced the deleterious effects of the growth retardation."}
{"id": "PMID:475087", "title": "Interferon induction in dogs.", "content": "A synthetic complex of poly (I)-poly (C) with poly-L-lysine and carboxymethyl cellulose (poly ICLC), as well as UV-inactivated Newcastle disease virus, B-1 strain, was used to induce interferon production in dogs. Several criteria were used for interferon specificity. The interferon response depended on dosage and route of inoculation. Serum interferon concentrations usually reached a peak by 8 hours after inoculation (AI), rapidly declined thereafter, and were nondetectable in most instances at 24 hours AI. Dogs responded less to interferon inducers when reinoculated 2 days after primary induction. The interferon response was biphasic (2 and 8 hours AI) when reinoculated 1 week after primary inoculation. Reinoculation 2 weeks after primary inoculation simulated the first response. Although both inducers caused severe lymphopenia in dogs, the toxic side effects would limit clinical use (in dogs) of poly ICLC, but not of UV-inactivated Newcastle disease virus.", "contents": "Interferon induction in dogs. A synthetic complex of poly (I)-poly (C) with poly-L-lysine and carboxymethyl cellulose (poly ICLC), as well as UV-inactivated Newcastle disease virus, B-1 strain, was used to induce interferon production in dogs. Several criteria were used for interferon specificity. The interferon response depended on dosage and route of inoculation. Serum interferon concentrations usually reached a peak by 8 hours after inoculation (AI), rapidly declined thereafter, and were nondetectable in most instances at 24 hours AI. Dogs responded less to interferon inducers when reinoculated 2 days after primary induction. The interferon response was biphasic (2 and 8 hours AI) when reinoculated 1 week after primary inoculation. Reinoculation 2 weeks after primary inoculation simulated the first response. Although both inducers caused severe lymphopenia in dogs, the toxic side effects would limit clinical use (in dogs) of poly ICLC, but not of UV-inactivated Newcastle disease virus."}
{"id": "PMID:475088", "title": "Halothane anesthesia in calves.", "content": "Because of the calf's popularity as an experimental animal and its often noted sensitivity to anesthetics and anesthesia, the potency of halothane was studied in eight, young (x = 5.85 weeks), healthy, male Holstein-Friesian calves. The minimal alveolar halothane-O2 concentration (MAC) which just prevented calf movement in response to a tail clamp was 0.76 +/- SEM 0.03 vol% and is less than predictions based on studies in man. The addition of 50% N2O to inspired gases decreased the halothane MAC to 0.59 +/- 0.03%. In the absence of common modifying factors of anesthesia, halothane-O2 caused cardiopulmonary depression in these calves in proportion to anesthetic dose. Only two (total protein and albumin) of 17 selected blood clinical biochemical values were significantly (P less than 0.05) altered from base line within seven days of anesthesia, indicating insults to major organ systems did not occur.", "contents": "Halothane anesthesia in calves. Because of the calf's popularity as an experimental animal and its often noted sensitivity to anesthetics and anesthesia, the potency of halothane was studied in eight, young (x = 5.85 weeks), healthy, male Holstein-Friesian calves. The minimal alveolar halothane-O2 concentration (MAC) which just prevented calf movement in response to a tail clamp was 0.76 +/- SEM 0.03 vol% and is less than predictions based on studies in man. The addition of 50% N2O to inspired gases decreased the halothane MAC to 0.59 +/- 0.03%. In the absence of common modifying factors of anesthesia, halothane-O2 caused cardiopulmonary depression in these calves in proportion to anesthetic dose. Only two (total protein and albumin) of 17 selected blood clinical biochemical values were significantly (P less than 0.05) altered from base line within seven days of anesthesia, indicating insults to major organ systems did not occur."}
{"id": "PMID:475090", "title": "Critical anthelmintic trials in ponies with oxfendazole and caviphos and concomitant studies on the spontaneous elimination of small strongylids.", "content": "The efficacy of the benzimidazole, oxfendazole, and the organophosphate, caviphos, against gastrointestinal parasites of ponies was evaluated by the critcial test method. Oxfendazole (10 mg/kg of body weight) given in single oral doses was 100% effective against adult large strongylids (Strongylus vulgaris, Strongylus edentatus, and Strongylus equinus), 99% effective against adult small strongylids, and 97% effective against 4th-stage small strongylids (genera identified in order of frequency: Cylicostephanus, Cyathostomum, Cylicocyclus, Triodontophorus, Poteriostomum, Oesophagodontus, Cylicodontophorus, Gyalocephalus, and Craterostomum). Caviphos (40 mg/kg of body weight) admixed in the grain ration (horse crunch) was 89% effective against adult large strongylids (S vulgaris and S edentatus) and 99% effective against adult small strongylids (genera identified earlier in order of frequency above), but only 35% effective against 4th-stage small strongylids. Both drugs were effective (100%) against adult and immature pinworms (Oxyuris equi) but ineffective against Habronema spp and Draschia megastoma. Oxfendazole was only 11% effective against stomach bots (Gasterophilus spp); caviphos was 73% effective against these species. During a three-day pretreatment interval, about 34% of the total population of small strongylids was lost spontaneously from the 29 ponies.", "contents": "Critical anthelmintic trials in ponies with oxfendazole and caviphos and concomitant studies on the spontaneous elimination of small strongylids. The efficacy of the benzimidazole, oxfendazole, and the organophosphate, caviphos, against gastrointestinal parasites of ponies was evaluated by the critcial test method. Oxfendazole (10 mg/kg of body weight) given in single oral doses was 100% effective against adult large strongylids (Strongylus vulgaris, Strongylus edentatus, and Strongylus equinus), 99% effective against adult small strongylids, and 97% effective against 4th-stage small strongylids (genera identified in order of frequency: Cylicostephanus, Cyathostomum, Cylicocyclus, Triodontophorus, Poteriostomum, Oesophagodontus, Cylicodontophorus, Gyalocephalus, and Craterostomum). Caviphos (40 mg/kg of body weight) admixed in the grain ration (horse crunch) was 89% effective against adult large strongylids (S vulgaris and S edentatus) and 99% effective against adult small strongylids (genera identified earlier in order of frequency above), but only 35% effective against 4th-stage small strongylids. Both drugs were effective (100%) against adult and immature pinworms (Oxyuris equi) but ineffective against Habronema spp and Draschia megastoma. Oxfendazole was only 11% effective against stomach bots (Gasterophilus spp); caviphos was 73% effective against these species. During a three-day pretreatment interval, about 34% of the total population of small strongylids was lost spontaneously from the 29 ponies."}
{"id": "PMID:475092", "title": "Effects of feeding cull domestic onions (Allium cepa) to sheep.", "content": "Twenty crossbred Panama yearling ewes were used to determine the effects of feeding cull onions (Allium cepa) to sheep. Ten test ewes were fed onions free choice with supplemntal alfalfa hay and grain, whereas ten control ewes were fed only alfalfa hay and grain. Anemia developed within 3 weeks in the test ewes, but was not seen in the controls. All ewes remained clinically normal during the study. Onions were fed for about 130 days. Recovery of the erythrocyte system began before the end of the feeding period. Even though onions were fed during most of the gestation period, the numbers of lambs born in the test and control groups were essentially the same.", "contents": "Effects of feeding cull domestic onions (Allium cepa) to sheep. Twenty crossbred Panama yearling ewes were used to determine the effects of feeding cull onions (Allium cepa) to sheep. Ten test ewes were fed onions free choice with supplemntal alfalfa hay and grain, whereas ten control ewes were fed only alfalfa hay and grain. Anemia developed within 3 weeks in the test ewes, but was not seen in the controls. All ewes remained clinically normal during the study. Onions were fed for about 130 days. Recovery of the erythrocyte system began before the end of the feeding period. Even though onions were fed during most of the gestation period, the numbers of lambs born in the test and control groups were essentially the same."}
{"id": "PMID:475093", "title": "Serum proteins of normal goats and goats with caseous lymphadenitis.", "content": "Values for total serum proteins and relative percentages of albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin, and gamma-globulin were determined for the goat. These normal values were compared with those obtained for goats infected with Corynebacterium pseudotuberculosis. Goats chronically infected with C pseudotuberculosis show significantly higher total serum protein values than normal goats, apparently due to increased gamma-globulins. This higher protein value is also associated with a decrease in serum alpha 2- and beta-globulins.", "contents": "Serum proteins of normal goats and goats with caseous lymphadenitis. Values for total serum proteins and relative percentages of albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin, and gamma-globulin were determined for the goat. These normal values were compared with those obtained for goats infected with Corynebacterium pseudotuberculosis. Goats chronically infected with C pseudotuberculosis show significantly higher total serum protein values than normal goats, apparently due to increased gamma-globulins. This higher protein value is also associated with a decrease in serum alpha 2- and beta-globulins."}
{"id": "PMID:475094", "title": "Emory milkvetch (Astragalus emoryanus var emoryanus) poisoning in chicks, sheep, and cattle.", "content": "A severe outbreak of Emory milkvetch poisoning in cattle and sheep occurred near Roswell, New Mexico, in the spring of 1975. Mortality averaged 2% to 3% and morbidity averaged 15% to 20%. Emory milkvetch collected from the infested area contained miserotoxin measured as 5 to 9 mg of NO2/g of plant (dry weight). Chicks fed extracts of Emory milkvetch showed toxic signs when fed one dose as 300 mg of NO2/kg of body weight, and died within 5 to 8 hours when fed milkvetch as 400 mg of NO2/kg. A sheep fed Emory milkvetch for 7 days in the form of 38 mg of NO2/kg/day developed signs of nitro poisoning on the 7th day. Cattle were poisoned or died when fed Emory milkvetch as 12 to 20 mg of NO2/ig for several days. The toxic signs observed in the field and under experimental conditions were similar.", "contents": "Emory milkvetch (Astragalus emoryanus var emoryanus) poisoning in chicks, sheep, and cattle. A severe outbreak of Emory milkvetch poisoning in cattle and sheep occurred near Roswell, New Mexico, in the spring of 1975. Mortality averaged 2% to 3% and morbidity averaged 15% to 20%. Emory milkvetch collected from the infested area contained miserotoxin measured as 5 to 9 mg of NO2/g of plant (dry weight). Chicks fed extracts of Emory milkvetch showed toxic signs when fed one dose as 300 mg of NO2/kg of body weight, and died within 5 to 8 hours when fed milkvetch as 400 mg of NO2/kg. A sheep fed Emory milkvetch for 7 days in the form of 38 mg of NO2/kg/day developed signs of nitro poisoning on the 7th day. Cattle were poisoned or died when fed Emory milkvetch as 12 to 20 mg of NO2/ig for several days. The toxic signs observed in the field and under experimental conditions were similar."}
{"id": "PMID:475096", "title": "Transmission of transmissible venereal tumor of the dog to the coyote.", "content": "Three coyotes experimentally implanted with cells from a transmissible venereal tumor of a dog developed neoplasms. Histopathologic appearances of the tumors in the coyotes were similar to those described in the dog. Attempts to transmit the tumor to suckling mice, rats, hamsters, kittens, and opossums were not successful.", "contents": "Transmission of transmissible venereal tumor of the dog to the coyote. Three coyotes experimentally implanted with cells from a transmissible venereal tumor of a dog developed neoplasms. Histopathologic appearances of the tumors in the coyotes were similar to those described in the dog. Attempts to transmit the tumor to suckling mice, rats, hamsters, kittens, and opossums were not successful."}
{"id": "PMID:475097", "title": "Epizootiologic features of canine hydrocephalus.", "content": "In a retrospective study of hydrocephalus (of congenital origin) diagnosed in 564 dogs, 11 breeds (including 8 \"toy\" breeds) were identified as being a significantly high risk. An inverse relationship between risk and body size was detected. The smaller the dam at maturity, either in height at the shoulder or body weight, the greater the risk of hydrocephalus in the offspring.", "contents": "Epizootiologic features of canine hydrocephalus. In a retrospective study of hydrocephalus (of congenital origin) diagnosed in 564 dogs, 11 breeds (including 8 \"toy\" breeds) were identified as being a significantly high risk. An inverse relationship between risk and body size was detected. The smaller the dam at maturity, either in height at the shoulder or body weight, the greater the risk of hydrocephalus in the offspring."}
{"id": "PMID:475098", "title": "Complement activity and conglutinin activity in stored bovine sera.", "content": "Complement activity and conglutinin activity were measured in bovine sera stored under four conditions: phenolized (3 C), lyophilized (3 C), frozen (-23 C), and frozen (-57 C). The complement activity was lost after 12 weeks in phenol (3 C). The other three conditions, ranked in order of increasing effectiveness, are lyophilized (3 C) and frozen (-23 and -57 C). Conglutinin activity was lost after 23 weeks in phenol (3 C). The other three conditions, ranked in order of increasing effectiveness, are frozen (-23 C), lyophilized (3 C) and frozen (-57 C). Thus, the most effective condition to preserve complement and conglutinin simultaneously is freezing at -57 C.", "contents": "Complement activity and conglutinin activity in stored bovine sera. Complement activity and conglutinin activity were measured in bovine sera stored under four conditions: phenolized (3 C), lyophilized (3 C), frozen (-23 C), and frozen (-57 C). The complement activity was lost after 12 weeks in phenol (3 C). The other three conditions, ranked in order of increasing effectiveness, are lyophilized (3 C) and frozen (-23 and -57 C). Conglutinin activity was lost after 23 weeks in phenol (3 C). The other three conditions, ranked in order of increasing effectiveness, are frozen (-23 C), lyophilized (3 C) and frozen (-57 C). Thus, the most effective condition to preserve complement and conglutinin simultaneously is freezing at -57 C."}
{"id": "PMID:475099", "title": "Evaluation of four forms of oxfendazole against nematodes of cattle.", "content": "Oxfendazole, methyl [5-(phenylsulfinyl)-1H-benzimidazol-2-yl] carbamate, was evaluated in calves naturally infected with endoparasites. The compound was used as a drench, a paste, a bolus, and as top-dress crumbles at a dosage of 2.5 mg/kg of body weight. The four formulations produced similar effects and were 99% to 100% effective in removing the following adult parasite genera: Haemonchus, Ostertagia, Trichostrongylus. Cooperia, Bunostomum, Oesophagostomum, and Dictyocaulus. Mean efficacy against Trichuris spp varied from 87% to 95%, depending on the formulation used.", "contents": "Evaluation of four forms of oxfendazole against nematodes of cattle. Oxfendazole, methyl [5-(phenylsulfinyl)-1H-benzimidazol-2-yl] carbamate, was evaluated in calves naturally infected with endoparasites. The compound was used as a drench, a paste, a bolus, and as top-dress crumbles at a dosage of 2.5 mg/kg of body weight. The four formulations produced similar effects and were 99% to 100% effective in removing the following adult parasite genera: Haemonchus, Ostertagia, Trichostrongylus. Cooperia, Bunostomum, Oesophagostomum, and Dictyocaulus. Mean efficacy against Trichuris spp varied from 87% to 95%, depending on the formulation used."}
{"id": "PMID:475100", "title": "Regulation of bovine labor with a long-acting carba-analog of oxytocin: a preliminary report.", "content": "The long-acting oxytocin (OT) analog 1-desamino-1-monocarba-E12-Tyr(OMe)]-OT(dCOMOT) was given IV to 13 pregnant cows near to term, but not in actual labor. The animals were para 1 to 5. Of these cows, four were treated with 20 mg of dexamethoasone 48 hours before the peptide was injected; the remaining nine animals were given no other medication. The animals usually were given a single injection of 5 mg of dCOMOT into the jugular veiw (5 to 7 micrograms/kg of body weight). In those instances where delivery was not complete within six hours, a second injection of peptide was given. In all instances, the first injection initiated labor as judged by the behavior of both uterus and cow. In most instances, there was also intermittent spurting of colostrum from the udder over a six-hour period after injection. In the four cows treated first with dexamethasone, the mean duration of induced labor was 4.35 hours after dCOMOT injection. In the nine non-treated cows, the mean duration of induced labor was 14.25 hours. The difference between the two values was significant. For both groups separately, and all data together, there was a linear inverse relation between the size of the external ostium uteri at injection and the duration of labor after peptide injection. All calves were healthy with no signs of hypoxia and the dose rates used did not result in any instance of uterine tetany or tachyphylaxis.", "contents": "Regulation of bovine labor with a long-acting carba-analog of oxytocin: a preliminary report. The long-acting oxytocin (OT) analog 1-desamino-1-monocarba-E12-Tyr(OMe)]-OT(dCOMOT) was given IV to 13 pregnant cows near to term, but not in actual labor. The animals were para 1 to 5. Of these cows, four were treated with 20 mg of dexamethoasone 48 hours before the peptide was injected; the remaining nine animals were given no other medication. The animals usually were given a single injection of 5 mg of dCOMOT into the jugular veiw (5 to 7 micrograms/kg of body weight). In those instances where delivery was not complete within six hours, a second injection of peptide was given. In all instances, the first injection initiated labor as judged by the behavior of both uterus and cow. In most instances, there was also intermittent spurting of colostrum from the udder over a six-hour period after injection. In the four cows treated first with dexamethasone, the mean duration of induced labor was 4.35 hours after dCOMOT injection. In the nine non-treated cows, the mean duration of induced labor was 14.25 hours. The difference between the two values was significant. For both groups separately, and all data together, there was a linear inverse relation between the size of the external ostium uteri at injection and the duration of labor after peptide injection. All calves were healthy with no signs of hypoxia and the dose rates used did not result in any instance of uterine tetany or tachyphylaxis."}
{"id": "PMID:475101", "title": "Actions of oxytocin and a long-acting carba oxytocin analog on the porcine myometrium in vitro and in vivo.", "content": "The contractile actions of oxytocin and its analog 1-desamino-1-monocarba-[2-Tyr(OMe)]-oxytocin were compared on in vitro (experiment 1) and in vivo (experiment 2) preparations of porcine uterine horns. In both experiments, sows in estrus were selected for maximal sensitivity. Despite the loss of peak amplitude associated with the substitution at sequence position 2 of O-methyltyrosine for tyrosine, the two remaining alterations (N-terminal desamination and replacement of the 1-6 S-S bridge by CH2S) prolonged the action of single-dose presentations. Thus, instead of having a response lasting minutes when the parent hormone is given, an IV dose of about 5 micrograms of the analog/kg of body weight in the intact anesthetized sow resulted in a response lasting two hours.", "contents": "Actions of oxytocin and a long-acting carba oxytocin analog on the porcine myometrium in vitro and in vivo. The contractile actions of oxytocin and its analog 1-desamino-1-monocarba-[2-Tyr(OMe)]-oxytocin were compared on in vitro (experiment 1) and in vivo (experiment 2) preparations of porcine uterine horns. In both experiments, sows in estrus were selected for maximal sensitivity. Despite the loss of peak amplitude associated with the substitution at sequence position 2 of O-methyltyrosine for tyrosine, the two remaining alterations (N-terminal desamination and replacement of the 1-6 S-S bridge by CH2S) prolonged the action of single-dose presentations. Thus, instead of having a response lasting minutes when the parent hormone is given, an IV dose of about 5 micrograms of the analog/kg of body weight in the intact anesthetized sow resulted in a response lasting two hours."}
{"id": "PMID:475102", "title": "Comparative efficacy of ten commercial Campylobacter fetus vaccines in the pregnant guinea pig: challenge with Campylobacter fetus serotype A.", "content": "Efficacy of ten commercial Campylobacter fetus vaccines was tested in pregnant guinea pigs and compared with that of an experimental vaccine prepared from the challenge-exposure strain. If the first lot of vaccine failed to protect 50% of the guinea pigs, one or two additional lots of that vaccine were purchased and retested. Three vaccines for cattle, evaluated, as the most effective of those tested, protected 62%, 72%, and 89% of the guinea pigs from abortion; the experimental vaccine protected 98%. The two vaccines for sheep protected 50% and 61% of the guinea pigs from abortion. With the other five vaccines produced for immunizing cattle, protection was from 0% to 36%, with the exception of one lot of a vaccine that protected 74%. Blood infection was found at necropsy in only 6% of the guinea pigs given vaccines that protected 50% or more from abortion, but was found in 66% of those given vaccines that protected less than 50%. Similarly, tissue infection was found at necropsy in only 18% of the guinea pigs given vaccines that protected more than 50%, but was found in 91% of those given vaccines that protected less than 50% from abortion. Oil-emulsion adjuvants appeared to enhance protection from abortion and infection. Nodules persisted at the injection site in most of the guinea pigs immunized with vaccines containing oil-emulsion adjuvants, but rarely persisted in guinea pigs given aqueous-phase adjuvant vaccines. Comparison of efficacy of the vaccines in guinea pigs with efficacy in sheep and cattle remains to be made.", "contents": "Comparative efficacy of ten commercial Campylobacter fetus vaccines in the pregnant guinea pig: challenge with Campylobacter fetus serotype A. Efficacy of ten commercial Campylobacter fetus vaccines was tested in pregnant guinea pigs and compared with that of an experimental vaccine prepared from the challenge-exposure strain. If the first lot of vaccine failed to protect 50% of the guinea pigs, one or two additional lots of that vaccine were purchased and retested. Three vaccines for cattle, evaluated, as the most effective of those tested, protected 62%, 72%, and 89% of the guinea pigs from abortion; the experimental vaccine protected 98%. The two vaccines for sheep protected 50% and 61% of the guinea pigs from abortion. With the other five vaccines produced for immunizing cattle, protection was from 0% to 36%, with the exception of one lot of a vaccine that protected 74%. Blood infection was found at necropsy in only 6% of the guinea pigs given vaccines that protected 50% or more from abortion, but was found in 66% of those given vaccines that protected less than 50%. Similarly, tissue infection was found at necropsy in only 18% of the guinea pigs given vaccines that protected more than 50%, but was found in 91% of those given vaccines that protected less than 50% from abortion. Oil-emulsion adjuvants appeared to enhance protection from abortion and infection. Nodules persisted at the injection site in most of the guinea pigs immunized with vaccines containing oil-emulsion adjuvants, but rarely persisted in guinea pigs given aqueous-phase adjuvant vaccines. Comparison of efficacy of the vaccines in guinea pigs with efficacy in sheep and cattle remains to be made."}
{"id": "PMID:475103", "title": "A computerized system for retrieval of case information in a veterinary diagnostic laboratory.", "content": "Morbidity and mortality data are necessary bases for the decision-making processes relevant to allocation of public funds for animal disease diagnoses and research. A system for information storage and retrieval capable of handling diagnostic data such as results of microbiology, parasitology, necropsy, and histopathology as well as demographic data such as owner, species, sex, breed, or geographic origin of the animal is described. This information is available to veterinarians, epidemiologists, herdsmen, and others involved in disease prevention or control efforts. The system described utilizes natural language, thus overcoming difficulties encountered in systems with numerical intermediates. Used and revised for the last 10 years, the system described has proved useful for annual administrative quantitation of services performed. In fact, the Concordance Index serves as the annual report of the University of Missouri Veterinary Medical Diagnostic Laboratory. Having accurate detailed information on individual cases, as well as a variety of composite data, has been extremely helpful in the documentation necessary for attracting funding for study of specific disease states.", "contents": "A computerized system for retrieval of case information in a veterinary diagnostic laboratory. Morbidity and mortality data are necessary bases for the decision-making processes relevant to allocation of public funds for animal disease diagnoses and research. A system for information storage and retrieval capable of handling diagnostic data such as results of microbiology, parasitology, necropsy, and histopathology as well as demographic data such as owner, species, sex, breed, or geographic origin of the animal is described. This information is available to veterinarians, epidemiologists, herdsmen, and others involved in disease prevention or control efforts. The system described utilizes natural language, thus overcoming difficulties encountered in systems with numerical intermediates. Used and revised for the last 10 years, the system described has proved useful for annual administrative quantitation of services performed. In fact, the Concordance Index serves as the annual report of the University of Missouri Veterinary Medical Diagnostic Laboratory. Having accurate detailed information on individual cases, as well as a variety of composite data, has been extremely helpful in the documentation necessary for attracting funding for study of specific disease states."}
{"id": "PMID:475104", "title": "Improved method for obtaining streptomycin-dependent mutants from Pasteurella multocida and Pasteurella haemolytica, using N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "N-Methyl-N'-nitro-N-nitrosoguanidine was used in a modified and improved procedure to obtain streptomycin-dependent mutants of type A Pasteurella multocida and type 1 Pasteurella haemolytica. The relative virulence of these mutants was determined by mouse inoculation with and without streptomycin.", "contents": "Improved method for obtaining streptomycin-dependent mutants from Pasteurella multocida and Pasteurella haemolytica, using N-methyl-N'-nitro-N-nitrosoguanidine. N-Methyl-N'-nitro-N-nitrosoguanidine was used in a modified and improved procedure to obtain streptomycin-dependent mutants of type A Pasteurella multocida and type 1 Pasteurella haemolytica. The relative virulence of these mutants was determined by mouse inoculation with and without streptomycin."}
{"id": "PMID:475106", "title": "Cyanide intoxication in sheep: enhancement of efficacy of sodium nitrite, sodium thiosulfate, and cobaltous chloride.", "content": "For treatment of cyanide intoxication of ruminants, the present recommended doses of sodium nitrite (5 mg/kg of body weight) and sodium thiosulfate (25 to 50 mg/kg) are smaller than those recommended for other animals; the decrease is partially attributed to the greater susceptibility of ruminants to the toxic effects of sodium nitrite. Based on the high tissue concentration and activity rate of rhodanese in ruminants, sulfur donors such as sodium thiosulfate could be utilized more efficaciously. Doses of sodium nitrite and sodium thiosulfate (up to 22 and 660 mg/kg, respectively) were evaluated in the present studies. Adjustment of the antidotal combination provided almost three times the protection afforded by the previously recommended doses. Moreover, under the conditions tested, the newly adjusted dose levels of sodium thiosulfate alone were more effective than the previously used antidotal combination of sodium nitrite and sodium thiosulfate and this protective effect was enhanced by cobaltous chloride (10.6 mg/kg) or sodium nitrite. The present recommended therapeutic approach to cyanide intoxication in sheep should be based primarily on administration of a much higher dose of sodium thiosulfate in combination with sodium nitrite or cobaltous chloride (or both).", "contents": "Cyanide intoxication in sheep: enhancement of efficacy of sodium nitrite, sodium thiosulfate, and cobaltous chloride. For treatment of cyanide intoxication of ruminants, the present recommended doses of sodium nitrite (5 mg/kg of body weight) and sodium thiosulfate (25 to 50 mg/kg) are smaller than those recommended for other animals; the decrease is partially attributed to the greater susceptibility of ruminants to the toxic effects of sodium nitrite. Based on the high tissue concentration and activity rate of rhodanese in ruminants, sulfur donors such as sodium thiosulfate could be utilized more efficaciously. Doses of sodium nitrite and sodium thiosulfate (up to 22 and 660 mg/kg, respectively) were evaluated in the present studies. Adjustment of the antidotal combination provided almost three times the protection afforded by the previously recommended doses. Moreover, under the conditions tested, the newly adjusted dose levels of sodium thiosulfate alone were more effective than the previously used antidotal combination of sodium nitrite and sodium thiosulfate and this protective effect was enhanced by cobaltous chloride (10.6 mg/kg) or sodium nitrite. The present recommended therapeutic approach to cyanide intoxication in sheep should be based primarily on administration of a much higher dose of sodium thiosulfate in combination with sodium nitrite or cobaltous chloride (or both)."}
{"id": "PMID:475108", "title": "Trypanosoma theileri Laveran, 1902, in Wyoming cattle.", "content": "Trypanosoma theileri Laveran, 1902, a cosmopolitan blood parasite of cattle, was found in 86 of 377 Wyoming cattle. Acute infection was experimentally produced in 22 cattle, with blood stream trypanosomes reaching peaks (up to 8,00/ml) in the first 2 weeks of infection. Infection subsided after 3 to 4 weeks. In studies of animals with chronic infection, the parasites reappeared in the blood 4 to 6 months after initial exposure and again at 8 months. Morphologically, three trypomastigote forms were seen: short-thin forms, long-thin forms, and long-broad forms. It is considered that the first two types derived from the last. Comparisons of bloodstream trypomastigotes were made with chicken embryo culture forms, and it was concluded that identification and description of trypanosomes from cattle can be made only by examination of bloodstream stages.", "contents": "Trypanosoma theileri Laveran, 1902, in Wyoming cattle. Trypanosoma theileri Laveran, 1902, a cosmopolitan blood parasite of cattle, was found in 86 of 377 Wyoming cattle. Acute infection was experimentally produced in 22 cattle, with blood stream trypanosomes reaching peaks (up to 8,00/ml) in the first 2 weeks of infection. Infection subsided after 3 to 4 weeks. In studies of animals with chronic infection, the parasites reappeared in the blood 4 to 6 months after initial exposure and again at 8 months. Morphologically, three trypomastigote forms were seen: short-thin forms, long-thin forms, and long-broad forms. It is considered that the first two types derived from the last. Comparisons of bloodstream trypomastigotes were made with chicken embryo culture forms, and it was concluded that identification and description of trypanosomes from cattle can be made only by examination of bloodstream stages."}
{"id": "PMID:475109", "title": "Hemodynamic effects of unilateral segmented lumbar epidural analgesia in cattle.", "content": "Hemodynamic measurements, arterial and mixed venous blood gas tensions, and pH were determined in 12 adult cows (mean weight 538 kg) following the injection of 8 ml of 5% procaine between the first and the second lumber vertebra into the epidural space. Comparison of base-line data with obtained during unilateral segmented lumbar analgesia between T(13) and L(3) segments indicated significant decreases (P is less than 0.05) in total peripheral resistance and diastolic and mean arterial blood pressures, and significant increases (P is less than 0.05) in heart rate and cardiac output. Arterial and mixed venous blood gas tensions and pH, O(2)-uptake, stroke volume, left ventricular stroke work, left ventricular minute work and hematocrit did not change significantly (P is less than 0.05). Cardiovascular and respiratory values in two cows (mean weight 700 kg) given xylazine (50 mg, IM), were depressed from base-line data, but did not change significantly (P is less than 0.05) during unilateral segmental epidural analgesia of segments T(13 to L(3). The nonsedated healthy cow tolerates sympathetic vasomotor blockade between T(13) and L(3) segments well and is able to mobilize circulatory mechanisms effectively.", "contents": "Hemodynamic effects of unilateral segmented lumbar epidural analgesia in cattle. Hemodynamic measurements, arterial and mixed venous blood gas tensions, and pH were determined in 12 adult cows (mean weight 538 kg) following the injection of 8 ml of 5% procaine between the first and the second lumber vertebra into the epidural space. Comparison of base-line data with obtained during unilateral segmented lumbar analgesia between T(13) and L(3) segments indicated significant decreases (P is less than 0.05) in total peripheral resistance and diastolic and mean arterial blood pressures, and significant increases (P is less than 0.05) in heart rate and cardiac output. Arterial and mixed venous blood gas tensions and pH, O(2)-uptake, stroke volume, left ventricular stroke work, left ventricular minute work and hematocrit did not change significantly (P is less than 0.05). Cardiovascular and respiratory values in two cows (mean weight 700 kg) given xylazine (50 mg, IM), were depressed from base-line data, but did not change significantly (P is less than 0.05) during unilateral segmental epidural analgesia of segments T(13 to L(3). The nonsedated healthy cow tolerates sympathetic vasomotor blockade between T(13) and L(3) segments well and is able to mobilize circulatory mechanisms effectively."}
{"id": "PMID:475110", "title": "In vitro killing of Pasteurella multocida: the effect of rabbit granulocyte and specific antibody source.", "content": "In vitro granulocyte-killing assays were performed to examine the ability of granulocytes from pasteurella-free or immunized rabbits, the in combination with specific immune serum, to kill Pasteurella multocida. Granulocytes from healthy rabbits and from rabbits with P multocida infections were equally competent. Granulocyte source, serum source, and specific antibody titer had no effect on granulocyte phagocytic activity. Moreover, serum containing specific antibody and complement supported the growth of the bacterium. These data suggest that chronic P multocida infections are not attributable to defective granulocytes or lack of serum antibody production.", "contents": "In vitro killing of Pasteurella multocida: the effect of rabbit granulocyte and specific antibody source. In vitro granulocyte-killing assays were performed to examine the ability of granulocytes from pasteurella-free or immunized rabbits, the in combination with specific immune serum, to kill Pasteurella multocida. Granulocytes from healthy rabbits and from rabbits with P multocida infections were equally competent. Granulocyte source, serum source, and specific antibody titer had no effect on granulocyte phagocytic activity. Moreover, serum containing specific antibody and complement supported the growth of the bacterium. These data suggest that chronic P multocida infections are not attributable to defective granulocytes or lack of serum antibody production."}
{"id": "PMID:475111", "title": "Renal sodium and potassium excretion in sheep given amiloride.", "content": "When amiloride was given (IV) to unanesthetized ewes, potassium excretion decreased to one-third of baseline values, and sodium excretion increased 6- to 180-fold. Potassium excretion during amiloride administration was relatively invariant with respect to duration (0 to 270 minutes) or rate of amiloride administration (0.125 to 2.0 mg/minute), but sodium excretion clearly increased with both duration and dose rate in individual experiments. This increase was independent of the rate of concomitant saline administration. Thus, sheep fed a normal ration (about 600 mEq of potassium per day) respond to amiloride as do man, dogs, and rats. The relationship of sodium excretion to rate and duration of amiloride administration is not unique to sheep, but has not been stressed in previous studies on other species.", "contents": "Renal sodium and potassium excretion in sheep given amiloride. When amiloride was given (IV) to unanesthetized ewes, potassium excretion decreased to one-third of baseline values, and sodium excretion increased 6- to 180-fold. Potassium excretion during amiloride administration was relatively invariant with respect to duration (0 to 270 minutes) or rate of amiloride administration (0.125 to 2.0 mg/minute), but sodium excretion clearly increased with both duration and dose rate in individual experiments. This increase was independent of the rate of concomitant saline administration. Thus, sheep fed a normal ration (about 600 mEq of potassium per day) respond to amiloride as do man, dogs, and rats. The relationship of sodium excretion to rate and duration of amiloride administration is not unique to sheep, but has not been stressed in previous studies on other species."}
{"id": "PMID:475112", "title": "Sequestration and phagocytosis of Haemobartonella felis in the spleen.", "content": "Spleens of two cats infected with Haemobartonella felis were examined by electron microscopy to determine the means by which the organism was sequestered in this organ. The principal means of sequestration occurred when H felis, located on the erythrocytes was removed by phagocytosis by a cordal macrophage, apparently preceded by the adhesion of extended processes of the macrophage to H felis. The second and least frequent means of removal of H felis was by pitting, a process that did not cause destruction of the host erythrocyte. The H felis was pitted from the parasitized erythrocyte when H felis passed through gaps between reticular cells or when the parasitized erythrocyte passed among the cytoplasmic processes of the reticular cells in the splenic cords. Some H felis were closely associated with the plasmalemma of cordal reticular cells and also were located in intracytoplasmic vacuoles of the cells without being influenced by the phagocytic process.", "contents": "Sequestration and phagocytosis of Haemobartonella felis in the spleen. Spleens of two cats infected with Haemobartonella felis were examined by electron microscopy to determine the means by which the organism was sequestered in this organ. The principal means of sequestration occurred when H felis, located on the erythrocytes was removed by phagocytosis by a cordal macrophage, apparently preceded by the adhesion of extended processes of the macrophage to H felis. The second and least frequent means of removal of H felis was by pitting, a process that did not cause destruction of the host erythrocyte. The H felis was pitted from the parasitized erythrocyte when H felis passed through gaps between reticular cells or when the parasitized erythrocyte passed among the cytoplasmic processes of the reticular cells in the splenic cords. Some H felis were closely associated with the plasmalemma of cordal reticular cells and also were located in intracytoplasmic vacuoles of the cells without being influenced by the phagocytic process."}
{"id": "PMID:475113", "title": "Ventricular wall thickness in the fetal dog.", "content": "Ventricular wall thickness of 21 50-to 60-day canine fetuses was determined by direct measurement with calipers. Measurements were made of the left and right ventricular walls and of the interventricular septum, perpendicular to the apico-basilar axis at the location just below the aortic valve, and midway between the coronary sulcus and the apex. Mean determiniations were used to characterize the wall thickne-s at the various levels. The right wall was found to be thickpreciable thickness differences between the septal and left wall at this location. At the level of the midventricular wall, the septal wall thickness exceeded that of the left and right wall. Left and right wall thickness at this level were similar.", "contents": "Ventricular wall thickness in the fetal dog. Ventricular wall thickness of 21 50-to 60-day canine fetuses was determined by direct measurement with calipers. Measurements were made of the left and right ventricular walls and of the interventricular septum, perpendicular to the apico-basilar axis at the location just below the aortic valve, and midway between the coronary sulcus and the apex. Mean determiniations were used to characterize the wall thickne-s at the various levels. The right wall was found to be thickpreciable thickness differences between the septal and left wall at this location. At the level of the midventricular wall, the septal wall thickness exceeded that of the left and right wall. Left and right wall thickness at this level were similar."}
{"id": "PMID:475114", "title": "Effect of fenbendazole on Toxocara canis larvae in tissues of infected dogs.", "content": "The effect of fenbendazole therapy was studied in six dogs fed 10,000 embryonated Toxocara canis eggs. At 47 days after they were fed T canis, four dogs were given fenbendazole in two divided doses totaling 50 mg/kg of body weight each day for 14 days. Two infected dogs were not given fenbendazole. All dogs were necropsied at the end of treatment and the foci were counted in the lungs; their skeletal muscles were digested in 1% trypsin for the recovery of larvae. The T canis larvae were not recovered from the skeletal muscle of the four infected dogs treated with fenbendazole; 15 and 42 larvae/100 g of skeletal muscle were recovered from the two nonmedicated infeected dogs. The number of grossly visible foci on surfaces of lungs in treated dogs was markedly less than in the nonmedicated infected dogs. The results indicate that fenbendazole might be effective in preventing prenatal infection in dogs.", "contents": "Effect of fenbendazole on Toxocara canis larvae in tissues of infected dogs. The effect of fenbendazole therapy was studied in six dogs fed 10,000 embryonated Toxocara canis eggs. At 47 days after they were fed T canis, four dogs were given fenbendazole in two divided doses totaling 50 mg/kg of body weight each day for 14 days. Two infected dogs were not given fenbendazole. All dogs were necropsied at the end of treatment and the foci were counted in the lungs; their skeletal muscles were digested in 1% trypsin for the recovery of larvae. The T canis larvae were not recovered from the skeletal muscle of the four infected dogs treated with fenbendazole; 15 and 42 larvae/100 g of skeletal muscle were recovered from the two nonmedicated infeected dogs. The number of grossly visible foci on surfaces of lungs in treated dogs was markedly less than in the nonmedicated infected dogs. The results indicate that fenbendazole might be effective in preventing prenatal infection in dogs."}
{"id": "PMID:475115", "title": "Efficacy of injectable and tablet formulations of praziquantel against immature Echinococcus granulosus.", "content": "Efficacy of an injectable formulation of praziquantel (given intramuscularly or subcutaneously at a dosage level of 5 mg/kg of body weight) or a tablet formulation (5 mg/kg) against immature Echinococcus granulosus was studied in experimentally infected dogs. The compound was efficacious (100% clearance) if injected via either route or if administered orally.", "contents": "Efficacy of injectable and tablet formulations of praziquantel against immature Echinococcus granulosus. Efficacy of an injectable formulation of praziquantel (given intramuscularly or subcutaneously at a dosage level of 5 mg/kg of body weight) or a tablet formulation (5 mg/kg) against immature Echinococcus granulosus was studied in experimentally infected dogs. The compound was efficacious (100% clearance) if injected via either route or if administered orally."}
{"id": "PMID:475116", "title": "Evaluation of a soluble sustained-release ophthalmic delivery unit in the dog.", "content": "A 5-mg hydroxypropyl cellulose soluble ocular insert was evaluated by placement in the upper and the lower conjunctival and bulbar nictitating membrane fornices in normal dogs. The insert was easily placed in the conjunctival fornices, but was difficult to place in the bulbar nictitating membrane fornix. The conjunctival inserts did not produce irritation of the cornea and the conjunctiva. Placement of the inserts beneath the nictitating membrane produced local irritation and chemosis. The sites of highest to lowest retention for the insert were upper conjunctival fornix, bulbar nictitating membrane fornix, and lastly, lower conjunctival fornix. Dissolution was nearly complete in 8 hours.", "contents": "Evaluation of a soluble sustained-release ophthalmic delivery unit in the dog. A 5-mg hydroxypropyl cellulose soluble ocular insert was evaluated by placement in the upper and the lower conjunctival and bulbar nictitating membrane fornices in normal dogs. The insert was easily placed in the conjunctival fornices, but was difficult to place in the bulbar nictitating membrane fornix. The conjunctival inserts did not produce irritation of the cornea and the conjunctiva. Placement of the inserts beneath the nictitating membrane produced local irritation and chemosis. The sites of highest to lowest retention for the insert were upper conjunctival fornix, bulbar nictitating membrane fornix, and lastly, lower conjunctival fornix. Dissolution was nearly complete in 8 hours."}
{"id": "PMID:475117", "title": "Maintenance of physiologic concentrations of plasma testosterone in the castrated male dog, using testosterone-filled polydimethylsiloxane capsules.", "content": "Effects of various numbers of polydimethylsiloxane (PDS) capsules filled with testosterone (PDS-T) on plasma testosterone (PT) in castrated male dogs were studied. Dogs were implanted with 1 empty PDS capsule or 1, 3, or 5 PDS-T capsules. Blood samples were collected prior to and after implantation, after castration with capsules in situ, and after capsule removal. The PT was determined in these samples by radioimmunoassay. One empty capsule had no effect on PT concentration; after castration, PT values fell to nondetectable amounts. One PDS-T capsule maintained PT at concentrations above nondetectable amounts after castration, but these concentrations were significantly (P less than 0.05) lower than were preimplantation values. Three or five PDS-T capsules were capable of maintaining PT concentrations in the castrated male dog similar to those concentrations seen in the intact dog.", "contents": "Maintenance of physiologic concentrations of plasma testosterone in the castrated male dog, using testosterone-filled polydimethylsiloxane capsules. Effects of various numbers of polydimethylsiloxane (PDS) capsules filled with testosterone (PDS-T) on plasma testosterone (PT) in castrated male dogs were studied. Dogs were implanted with 1 empty PDS capsule or 1, 3, or 5 PDS-T capsules. Blood samples were collected prior to and after implantation, after castration with capsules in situ, and after capsule removal. The PT was determined in these samples by radioimmunoassay. One empty capsule had no effect on PT concentration; after castration, PT values fell to nondetectable amounts. One PDS-T capsule maintained PT at concentrations above nondetectable amounts after castration, but these concentrations were significantly (P less than 0.05) lower than were preimplantation values. Three or five PDS-T capsules were capable of maintaining PT concentrations in the castrated male dog similar to those concentrations seen in the intact dog."}
{"id": "PMID:475118", "title": "Evaluation of methods for localization of urinary tract infection in the female dog.", "content": "Urinary tract infection (UTI) was induced in the left kidney of seven female dogs and in the urinary bladder of eight female dogs. Several methods advocated for localization of UTI in other species were tested in the infected dogs. Although fever, renal pain, and leukocytosis were detected in some dogs with renal infection, findings were transient. Radiographic changes in the kidneys and ureters were detected in some dogs with renal infection, but were absent in others. Bladder washout studies were not reliable for differentiating renal infection from bladder infection. Antibody coating studies were positive in dogs with bladder infection and in dogs with renal infection. The positive results from dogs with bladder infection may have been because of nonspecific binding of immunoglobulins to Staphylococcus aureus after leakage of serum immunoglobulins into urine. Studies of six dogs of both sexes with naturally occurring UTI indicated that their serum contained antibody against common urinary pathogens and that this antibody gained access to the urine in some dogs. It was concluded that the antibody coating test was unreliable for localization of UTI in the dog.", "contents": "Evaluation of methods for localization of urinary tract infection in the female dog. Urinary tract infection (UTI) was induced in the left kidney of seven female dogs and in the urinary bladder of eight female dogs. Several methods advocated for localization of UTI in other species were tested in the infected dogs. Although fever, renal pain, and leukocytosis were detected in some dogs with renal infection, findings were transient. Radiographic changes in the kidneys and ureters were detected in some dogs with renal infection, but were absent in others. Bladder washout studies were not reliable for differentiating renal infection from bladder infection. Antibody coating studies were positive in dogs with bladder infection and in dogs with renal infection. The positive results from dogs with bladder infection may have been because of nonspecific binding of immunoglobulins to Staphylococcus aureus after leakage of serum immunoglobulins into urine. Studies of six dogs of both sexes with naturally occurring UTI indicated that their serum contained antibody against common urinary pathogens and that this antibody gained access to the urine in some dogs. It was concluded that the antibody coating test was unreliable for localization of UTI in the dog."}
{"id": "PMID:475119", "title": "Pharmacokinetic study of sulfadimethoxine depletion in suckling and growing pigs.", "content": "Sulfadimethoxine was administered (IV) to suckling pigs (1 to 2 weeks old) and to growing pigs (11 to 12 weeks old) at a dosage of 55 mg/kg of body weight (single dose). Blood samples were collected over a 48-hour period, and the animals euthanatized were used for measurements of plasma and tissue concentrations of the drug. The blood data were described, using a one-compartment pharmacokinetic model. The blood concentration curves for the two groups of pigs had a consistent depletion pattern with greater than therapeutic concentrations (50 micrograms/ml) of the drug persisting through 12 hours after the drug was given. Sulfonamide blood concentrations were 4 and 11 times that of the method sensitivities in the older (growing) and younger (suckling) pigs, respectively, at 48 hours after treatment. In four of the five pharmacokinetic variables studied, a significantly higher (P less than 0.01) degree of efficiency was observed in the ability of the older pigs to eliminate the drug than in the younger pigs.", "contents": "Pharmacokinetic study of sulfadimethoxine depletion in suckling and growing pigs. Sulfadimethoxine was administered (IV) to suckling pigs (1 to 2 weeks old) and to growing pigs (11 to 12 weeks old) at a dosage of 55 mg/kg of body weight (single dose). Blood samples were collected over a 48-hour period, and the animals euthanatized were used for measurements of plasma and tissue concentrations of the drug. The blood data were described, using a one-compartment pharmacokinetic model. The blood concentration curves for the two groups of pigs had a consistent depletion pattern with greater than therapeutic concentrations (50 micrograms/ml) of the drug persisting through 12 hours after the drug was given. Sulfonamide blood concentrations were 4 and 11 times that of the method sensitivities in the older (growing) and younger (suckling) pigs, respectively, at 48 hours after treatment. In four of the five pharmacokinetic variables studied, a significantly higher (P less than 0.01) degree of efficiency was observed in the ability of the older pigs to eliminate the drug than in the younger pigs."}
{"id": "PMID:475120", "title": "Appearance of 15N-labeled intestinal microbial amino acids in the venous blood of the pig colon.", "content": "Two experiments were done to determine whether pigs possess the ability to absorb amino acids synthesized from urea nitrogen by indigenous microbes in the large intestine. Incorporation of [15N]urea into amino acid fractions of bacterial cells from the rectum and of the deproteinized incubated medium were examined in an experiment in vitro. The isotope was incorporated into 17 amino acids and the ammonia fraction of these samples. The absorption of the microbial amino acids from the colon was investigated by determination of the 15N concentration of the free amino acids in the venous blood of the colon after infusion of the 15N-labeled microorganisms into the cecum. The increase of 15N concentration was also observed in the plasma-free amino acids (threonine, isoleucine, phenylalanine, lysine, histidine, arginine, aspartic acid, serine, alanine, cystine) of the blood from the colic branch of the ileocolic vein. The results of these experiments indicated that pigs have the ability to utilize the microbial amino acids synthesized from urea nitrogen in the large intestine.", "contents": "Appearance of 15N-labeled intestinal microbial amino acids in the venous blood of the pig colon. Two experiments were done to determine whether pigs possess the ability to absorb amino acids synthesized from urea nitrogen by indigenous microbes in the large intestine. Incorporation of [15N]urea into amino acid fractions of bacterial cells from the rectum and of the deproteinized incubated medium were examined in an experiment in vitro. The isotope was incorporated into 17 amino acids and the ammonia fraction of these samples. The absorption of the microbial amino acids from the colon was investigated by determination of the 15N concentration of the free amino acids in the venous blood of the colon after infusion of the 15N-labeled microorganisms into the cecum. The increase of 15N concentration was also observed in the plasma-free amino acids (threonine, isoleucine, phenylalanine, lysine, histidine, arginine, aspartic acid, serine, alanine, cystine) of the blood from the colic branch of the ileocolic vein. The results of these experiments indicated that pigs have the ability to utilize the microbial amino acids synthesized from urea nitrogen in the large intestine."}
{"id": "PMID:475122", "title": "Method for repeated or prolonged rumen infusion without establishing an open fistula.", "content": "A method was developed for repeated or prolonged infusion of substances into the rumen. A blind rumen fistula was surgically formed so that the unopened rumen compartment was easily accessible immediately beneath the skin. A cardiac catheter was inserted into the rumen and was tied to a canvas patch cemented to the animal's coat. This method has been used in cattle and sheep for continuous infusion periods of one to six weeks. This surgical preparation also has been used to put plant material directly into the rumen and to obtain rumen contents. Thus, a large number of fistulated animals can be maintained over long periods without the disadvantages of maintenance required for animals each having an open fistula fitted with a closure device.", "contents": "Method for repeated or prolonged rumen infusion without establishing an open fistula. A method was developed for repeated or prolonged infusion of substances into the rumen. A blind rumen fistula was surgically formed so that the unopened rumen compartment was easily accessible immediately beneath the skin. A cardiac catheter was inserted into the rumen and was tied to a canvas patch cemented to the animal's coat. This method has been used in cattle and sheep for continuous infusion periods of one to six weeks. This surgical preparation also has been used to put plant material directly into the rumen and to obtain rumen contents. Thus, a large number of fistulated animals can be maintained over long periods without the disadvantages of maintenance required for animals each having an open fistula fitted with a closure device."}
{"id": "PMID:475123", "title": "Reference values for a field test to estimate inadequate glutathione peroxidase activity and selenium status in the blood of cattle.", "content": "A clinical screening procedure was developed for estimating glutathione peroxidase (GSH-Px) activity and selenium status in the blood of dairy and beef cattle. The test is based on the rate of defluorescence under long waveform UV irradiation of reduced nicotinamide adenine dinucleotide phosphate in a coupled enzyme reaction involving glutathione reductase. Defluorescence rates were significantly correlated with blood GSH-Px activity and blood selenium concentrations as determined by conventional laboratory procedures. Blood selenium concentrations and blood GSH-Px activity in several herds were compared with the selenium content of the complete ration consumed to establish reference or base-line values for these blood characteristics under field conditions. The GSH-Px screening procedure provides rapid results, is relatively inexpensive, and appears to be useful in differentiating between the reference values and grossly inadequate selenium status in cattle.", "contents": "Reference values for a field test to estimate inadequate glutathione peroxidase activity and selenium status in the blood of cattle. A clinical screening procedure was developed for estimating glutathione peroxidase (GSH-Px) activity and selenium status in the blood of dairy and beef cattle. The test is based on the rate of defluorescence under long waveform UV irradiation of reduced nicotinamide adenine dinucleotide phosphate in a coupled enzyme reaction involving glutathione reductase. Defluorescence rates were significantly correlated with blood GSH-Px activity and blood selenium concentrations as determined by conventional laboratory procedures. Blood selenium concentrations and blood GSH-Px activity in several herds were compared with the selenium content of the complete ration consumed to establish reference or base-line values for these blood characteristics under field conditions. The GSH-Px screening procedure provides rapid results, is relatively inexpensive, and appears to be useful in differentiating between the reference values and grossly inadequate selenium status in cattle."}
{"id": "PMID:475124", "title": "Thermal inactivation of hog cholera virus in ham.", "content": "Experiments were conducted to determine the temperatures required to inactivate hog cholera virus (HCV) in fresh ham after 1 minute and in cured and processed (canned) ham after 90 minutes. A momentary or \"flash\" temperature of 71 C for 1 minute caused inactivation of the virus in 15 of 15 cubes (2 cm3) of ham. Hog cholera virus was destroyed in 21 of 21 canned hams (weighing 0.91 kg each) when an internal temperature of 65 C was sustained for 90 minutes. Pigs were found to be more sensitive than tissue culture cells for detecting viable HCV in heat-processed fresh hams. Virus was isolated by tissue culture technique only from those hams exposed to temperatures below 61 C. The relative concentration of HCV in unheated cured hams of experimentally infected pigs varied over a wide range; these pigs were inoculated with the virulent Ames strain and were killed on postinfection day 6 or 7.", "contents": "Thermal inactivation of hog cholera virus in ham. Experiments were conducted to determine the temperatures required to inactivate hog cholera virus (HCV) in fresh ham after 1 minute and in cured and processed (canned) ham after 90 minutes. A momentary or \"flash\" temperature of 71 C for 1 minute caused inactivation of the virus in 15 of 15 cubes (2 cm3) of ham. Hog cholera virus was destroyed in 21 of 21 canned hams (weighing 0.91 kg each) when an internal temperature of 65 C was sustained for 90 minutes. Pigs were found to be more sensitive than tissue culture cells for detecting viable HCV in heat-processed fresh hams. Virus was isolated by tissue culture technique only from those hams exposed to temperatures below 61 C. The relative concentration of HCV in unheated cured hams of experimentally infected pigs varied over a wide range; these pigs were inoculated with the virulent Ames strain and were killed on postinfection day 6 or 7."}
{"id": "PMID:475125", "title": "Critical tests and clinical trials on oxibendazole in horses with special reference to removal of Parascaris equorum.", "content": "The efficacy of oxibendazole given at dose level of 10 mg/kg of body weight was determined by 10 critical tests in foals and by 2 clinical trials in 20 foals (16 treated, 4 nontreated), with special interest in the drug activity against Parascaris equorum. The drug was uniformly efficacious (100%) against P equorum in the 10 critical-test foals, each having between 22 and 236 ascarids. Posttreatment reductions of ascarid egg counts in fecal samples were also 100% in suckling foals treated with oxibendazole given as a drench. Ascarid eggs did not reappear in fecal samples until the 8th week after foals were treated. A paste formulation of oxibendazole at 10 mg/kg also eliminated ascarid eggs from feces of 12 suckling foals. Strongyle EPG were markedly reduced by oxibendazole in the 10 critical-test foals and in 16 treated sucklings in the clinical trials. Egg and larval count data on foals in both the critical tests and the clinical trials also indicated oxibendazole was active against Strongyloides westeri. Untoward effects of treatment with oxibendazole were not observed.", "contents": "Critical tests and clinical trials on oxibendazole in horses with special reference to removal of Parascaris equorum. The efficacy of oxibendazole given at dose level of 10 mg/kg of body weight was determined by 10 critical tests in foals and by 2 clinical trials in 20 foals (16 treated, 4 nontreated), with special interest in the drug activity against Parascaris equorum. The drug was uniformly efficacious (100%) against P equorum in the 10 critical-test foals, each having between 22 and 236 ascarids. Posttreatment reductions of ascarid egg counts in fecal samples were also 100% in suckling foals treated with oxibendazole given as a drench. Ascarid eggs did not reappear in fecal samples until the 8th week after foals were treated. A paste formulation of oxibendazole at 10 mg/kg also eliminated ascarid eggs from feces of 12 suckling foals. Strongyle EPG were markedly reduced by oxibendazole in the 10 critical-test foals and in 16 treated sucklings in the clinical trials. Egg and larval count data on foals in both the critical tests and the clinical trials also indicated oxibendazole was active against Strongyloides westeri. Untoward effects of treatment with oxibendazole were not observed."}
{"id": "PMID:475127", "title": "Comparative lethal effects on mice of ruminal fluid from cattle fed hay or grain.", "content": "Strained ruminal fluid from cattle fed hay or grain was lethal to mice when injected intraperitoneally, but the fluid from grain-fed cattle was approximately 3.7 times more toxic than that from hay-fed cattle. The lethal factor(s) was not resistant to heat, was nondialyzable, was retained on Seitz and membrane filters, and was associated with the bacterial fraction of ruminal fluid. We concluded that death of the mice resulted from infection produced by facultative bacteria normally in ruminal fluid. Ruminal fluid from grain-fed cattle contained a greater number of facultative bacteria than did that from hay-fed cattle.", "contents": "Comparative lethal effects on mice of ruminal fluid from cattle fed hay or grain. Strained ruminal fluid from cattle fed hay or grain was lethal to mice when injected intraperitoneally, but the fluid from grain-fed cattle was approximately 3.7 times more toxic than that from hay-fed cattle. The lethal factor(s) was not resistant to heat, was nondialyzable, was retained on Seitz and membrane filters, and was associated with the bacterial fraction of ruminal fluid. We concluded that death of the mice resulted from infection produced by facultative bacteria normally in ruminal fluid. Ruminal fluid from grain-fed cattle contained a greater number of facultative bacteria than did that from hay-fed cattle."}
{"id": "PMID:475128", "title": "Infection and immune kinetics of Trichostrongylus axei in calves.", "content": "In one experiment, 21 calves were given daily oral immunizing inoculations of 1,000 infective larvae (L3) of Trichostrongylus axei, an abomasal nematode parasites, for 35 weeks. Calves were euthanatized in groups of three every 5 weeks to determine infection kinetics. Worm populations steadily increased through week 30, but the percentage of total inoculum that became established was about the same through week 30. At week 35, the number of worms dropped markedly. In a second experiment, 27 calves given daily oral inoculations of infective larvae were allotted to three groups comprised of three subgroups each: (A), challenge-exposed vaccinated; (B), nonchallenge-exposed vaccinated; and (C), challenge-exposed nonvaccinated. Calves of subgroups A and C were given single challenge inoculums of 200,000 L3 after the 10th, 20th, and 30th weeks. All calves were necropsied 35 days after challenge exposure. When immunity was determined from the equation: [(No. of worms in [(B + C)-A])/No. of worms in [B + C])] X 100, immunity was 35% at 10 weeks, 52% at 20 weeks, and 100% at 30 weeks.", "contents": "Infection and immune kinetics of Trichostrongylus axei in calves. In one experiment, 21 calves were given daily oral immunizing inoculations of 1,000 infective larvae (L3) of Trichostrongylus axei, an abomasal nematode parasites, for 35 weeks. Calves were euthanatized in groups of three every 5 weeks to determine infection kinetics. Worm populations steadily increased through week 30, but the percentage of total inoculum that became established was about the same through week 30. At week 35, the number of worms dropped markedly. In a second experiment, 27 calves given daily oral inoculations of infective larvae were allotted to three groups comprised of three subgroups each: (A), challenge-exposed vaccinated; (B), nonchallenge-exposed vaccinated; and (C), challenge-exposed nonvaccinated. Calves of subgroups A and C were given single challenge inoculums of 200,000 L3 after the 10th, 20th, and 30th weeks. All calves were necropsied 35 days after challenge exposure. When immunity was determined from the equation: [(No. of worms in [(B + C)-A])/No. of worms in [B + C])] X 100, immunity was 35% at 10 weeks, 52% at 20 weeks, and 100% at 30 weeks."}
{"id": "PMID:475129", "title": "Characterization of the inclusion limiting membrane of anaplasma marginale by immunoferritin labeling.", "content": "Purified anti-erythrocytic membrane antibody (PAMA) was prepared from rabbit anti-bovine erythrocyte serum by an adsorption and elution technique, utilizing bovine erythrocytes. Lysed and washed anaplasma-infected erythrocytes were incubated with PAMA or control reagents. Specimens were then subjected to immunoferritin labeling with ferritin antiglobulin conjugate. Upon examination by electron microscopy, specimens incubated with PAMA showed heavy ferritin labeling of erythrocytic membranes and also the limiting membranes of anaplasmal inclusions. Anaplasmal initial bodies freed from their inclusion membranes were not labeled. Negative control specimens, incubated with normal rabbit serum or PAMA which had been absorbed with erythrocytes, did not show specific ferritin labeling. Intact bovine erythrocytes, which were used as a positive control of anti-bovine erythrocytic membrane specificity, were heavily ferritin-labeled. Avian erythrocytes, a negative control of specificity, remained unlabeled. The results of this study indicate that the limiting membrane of the anaplasmal inclusion is derived from the erythrocytic membrane.", "contents": "Characterization of the inclusion limiting membrane of anaplasma marginale by immunoferritin labeling. Purified anti-erythrocytic membrane antibody (PAMA) was prepared from rabbit anti-bovine erythrocyte serum by an adsorption and elution technique, utilizing bovine erythrocytes. Lysed and washed anaplasma-infected erythrocytes were incubated with PAMA or control reagents. Specimens were then subjected to immunoferritin labeling with ferritin antiglobulin conjugate. Upon examination by electron microscopy, specimens incubated with PAMA showed heavy ferritin labeling of erythrocytic membranes and also the limiting membranes of anaplasmal inclusions. Anaplasmal initial bodies freed from their inclusion membranes were not labeled. Negative control specimens, incubated with normal rabbit serum or PAMA which had been absorbed with erythrocytes, did not show specific ferritin labeling. Intact bovine erythrocytes, which were used as a positive control of anti-bovine erythrocytic membrane specificity, were heavily ferritin-labeled. Avian erythrocytes, a negative control of specificity, remained unlabeled. The results of this study indicate that the limiting membrane of the anaplasmal inclusion is derived from the erythrocytic membrane."}
{"id": "PMID:475130", "title": "Cardiovascular effects of intravenous administration of propylene glycol and of oxytetracycline in propylene glycol in calves.", "content": "Comparisons were made of the acute cardiovascular effects of oxytetracycline, oxytetracycline in propylene glycol, and propylene glycol alone given to conscious dairy calves. The calves were chronically instrumented with intravascular catheters and electromagnetic flowmeter transducers in and on the pulmonary and renal arteries. Injection (IV) of aqueous preparations of oxytetracycline produced no statistically significant (P greater than 0.05) cardiocirculatory changes in these calves. Oxytetracycline in propylene glycol and propylene glycol alone both produced transient (1 to 4 minute) periods of cardiovascular depression characterized by cardiac asystole, systemic hypotension, and decreased pulmonary and renal arterial blood flow. The two preparations, in equivalent doses and volumes, produced statistically similar hemodynamic changes in the calves. The data from this study support the conclusion that the monitored cardiovascular effects of the commercially available oxytetracycline in propylene glycol in the intact, awake calves were due to the solvent propylene glycol. This conclusion is consistent with reports of other injectable products containing the same solvent.", "contents": "Cardiovascular effects of intravenous administration of propylene glycol and of oxytetracycline in propylene glycol in calves. Comparisons were made of the acute cardiovascular effects of oxytetracycline, oxytetracycline in propylene glycol, and propylene glycol alone given to conscious dairy calves. The calves were chronically instrumented with intravascular catheters and electromagnetic flowmeter transducers in and on the pulmonary and renal arteries. Injection (IV) of aqueous preparations of oxytetracycline produced no statistically significant (P greater than 0.05) cardiocirculatory changes in these calves. Oxytetracycline in propylene glycol and propylene glycol alone both produced transient (1 to 4 minute) periods of cardiovascular depression characterized by cardiac asystole, systemic hypotension, and decreased pulmonary and renal arterial blood flow. The two preparations, in equivalent doses and volumes, produced statistically similar hemodynamic changes in the calves. The data from this study support the conclusion that the monitored cardiovascular effects of the commercially available oxytetracycline in propylene glycol in the intact, awake calves were due to the solvent propylene glycol. This conclusion is consistent with reports of other injectable products containing the same solvent."}
{"id": "PMID:475131", "title": "Ultrastruct of the juxtaglomerular apparatus in the dog in a sodium-balanced state.", "content": "The ultrastructure of juxtaglomerular apparatuses (JGA) from four dogs in a state of sodium balance were examined. Plasma renin activity and urinary sodium excretion in the dogs were within normal values. At the vascular pole of the glomerulus, circumferentially oriented smooth muscle cells in the wall of the glomerular arteriole were abruptly replaced by modified smooth muscle cells with complicated interwoven cell processes. These modified cells were indistinguishable from the juxtaglomerular cells of the lacis region (extraglomerular mesangium). The basal lamina surrounding these modified smooth muscle cells in the wall of the glomerular arteriole was continuous with the basal lamina of the juxtaglomerular cells of the lacis (extraglomerular mesangium) and the macula densa. The most striking differences in the JGA of the dog, when compared with the JGA of other laboratory animals, are related to the observation that cytoplasmic secretory granules are less abundant in the dog, are generally smaller than the practical resolving capability of the light microscope, and are restricted to cells of the lacis area in the JGA.", "contents": "Ultrastruct of the juxtaglomerular apparatus in the dog in a sodium-balanced state. The ultrastructure of juxtaglomerular apparatuses (JGA) from four dogs in a state of sodium balance were examined. Plasma renin activity and urinary sodium excretion in the dogs were within normal values. At the vascular pole of the glomerulus, circumferentially oriented smooth muscle cells in the wall of the glomerular arteriole were abruptly replaced by modified smooth muscle cells with complicated interwoven cell processes. These modified cells were indistinguishable from the juxtaglomerular cells of the lacis region (extraglomerular mesangium). The basal lamina surrounding these modified smooth muscle cells in the wall of the glomerular arteriole was continuous with the basal lamina of the juxtaglomerular cells of the lacis (extraglomerular mesangium) and the macula densa. The most striking differences in the JGA of the dog, when compared with the JGA of other laboratory animals, are related to the observation that cytoplasmic secretory granules are less abundant in the dog, are generally smaller than the practical resolving capability of the light microscope, and are restricted to cells of the lacis area in the JGA."}
{"id": "PMID:475132", "title": "Pathophysiologic response of the juxtaglomerular apparatus to dietary sodium restriction in the dog.", "content": "Pathophysiologic changes in the juxtaglomerular apparatus (JGA) of the dog were induced by 10 days of dietary sodium restriction (less than 1 mEq of Na+/day). Plasma renin activity increased 12-fold and plasma aldosterone values increased 60-fold, whereas urinary sodium excretion decreased precipitously. Urinary potassium excretion remained within normal values throughout the period of sodium restriction. The JGA cell counts, determined by light microscopy, were significantly (P = less than 0.05) increased after 2 days of sodium restriction and remained increased through day 10. Adrenal gland weights and the cross-sectional width of the zona glomerulosa were not altered. Ultrastructurally, JGA cells showed progressive hypertrophy and hyperplasia. The Golgi apparatus became more prominent. The endoplasmic reticulum increased, as did the number of ribosomes. Cytoplasmic secretory granules increased in number and size from day 2 through day 6. On days 8 and 10, fewer and smaller secretory granules were encountered, even though plasma renin and aldosterone values continued to increase. In the dog maintained in a balanced sodium state, little renin is stored in cytoplasmic granules of the juxtaglomerular cells. Short-term stimulation results in increased plasma renin values and increased production and storage of renin in JGA cells. Continued stimulation results in depletion of cytoplasmic stores, although plasma renin content continues to rise, suggesting that renin is produced and secreted directly during more prolonged stimulation.", "contents": "Pathophysiologic response of the juxtaglomerular apparatus to dietary sodium restriction in the dog. Pathophysiologic changes in the juxtaglomerular apparatus (JGA) of the dog were induced by 10 days of dietary sodium restriction (less than 1 mEq of Na+/day). Plasma renin activity increased 12-fold and plasma aldosterone values increased 60-fold, whereas urinary sodium excretion decreased precipitously. Urinary potassium excretion remained within normal values throughout the period of sodium restriction. The JGA cell counts, determined by light microscopy, were significantly (P = less than 0.05) increased after 2 days of sodium restriction and remained increased through day 10. Adrenal gland weights and the cross-sectional width of the zona glomerulosa were not altered. Ultrastructurally, JGA cells showed progressive hypertrophy and hyperplasia. The Golgi apparatus became more prominent. The endoplasmic reticulum increased, as did the number of ribosomes. Cytoplasmic secretory granules increased in number and size from day 2 through day 6. On days 8 and 10, fewer and smaller secretory granules were encountered, even though plasma renin and aldosterone values continued to increase. In the dog maintained in a balanced sodium state, little renin is stored in cytoplasmic granules of the juxtaglomerular cells. Short-term stimulation results in increased plasma renin values and increased production and storage of renin in JGA cells. Continued stimulation results in depletion of cytoplasmic stores, although plasma renin content continues to rise, suggesting that renin is produced and secreted directly during more prolonged stimulation."}
{"id": "PMID:475133", "title": "Thyroid gland and arterial lesions of Beagles with familial hypothyroidism and hyperlipoproteinemia.", "content": "Primary hypothyroidism with marked hyperlipoproteinemia and modest to severe atherosclerosis was described in a family of Beagles. The disease was familial and probably hereditary, but the mode of inheritance was unknown. Signs of the disease occurred as early as 1.5 years of age and as late as 5 years of age. The onset of the disease was heralded by reduced concentrations of serum thyroxine, increased binding capacity of thyroxine-binding globulin, and reduced response to injected thyroxine-stimulating hormone. Thereafter, serum lipids, particularly serum cholesterol, increased markedly even though the dogs consumed a diet low in fats and cholesterol. Thyroxine administration resulted in a precipitous decline in serum cholesterol concentrations. Microscopic changes in the thyroid glands included neoplastic transformation, adenomatous hyperplasia, lymphocytic thyroiditis, and atrophy. Moderate to severe atherosclerosis occurred mainly in the coronary and renal arteries. Atherosclerotic arteries were stenotic but patent, and there was no evidence of prior occlusion or thrombosis.", "contents": "Thyroid gland and arterial lesions of Beagles with familial hypothyroidism and hyperlipoproteinemia. Primary hypothyroidism with marked hyperlipoproteinemia and modest to severe atherosclerosis was described in a family of Beagles. The disease was familial and probably hereditary, but the mode of inheritance was unknown. Signs of the disease occurred as early as 1.5 years of age and as late as 5 years of age. The onset of the disease was heralded by reduced concentrations of serum thyroxine, increased binding capacity of thyroxine-binding globulin, and reduced response to injected thyroxine-stimulating hormone. Thereafter, serum lipids, particularly serum cholesterol, increased markedly even though the dogs consumed a diet low in fats and cholesterol. Thyroxine administration resulted in a precipitous decline in serum cholesterol concentrations. Microscopic changes in the thyroid glands included neoplastic transformation, adenomatous hyperplasia, lymphocytic thyroiditis, and atrophy. Moderate to severe atherosclerosis occurred mainly in the coronary and renal arteries. Atherosclerotic arteries were stenotic but patent, and there was no evidence of prior occlusion or thrombosis."}
{"id": "PMID:475134", "title": "Transmission and control of Filaroides hirthi lungworm infection in dogs.", "content": "Transmission of Filaroides hirthi among cagemate pups was demonstrated to occur through the ingestion of first-stage larvae in recently passed feces. It was suggested that coprophagia is the principal mechanism of transmission of F hirthi and that transmission from brood bitches to their litters occurs by this mechanism during and after the fourth or fifth week of the nursing period. Medication of 15 infected brood bitches with two courses of albendazole at a dosage rate of 50 mg/kg of body weight twice each day for 5 days resulted in complete absence of F hirthi infection among all 21 of their progeny, whereas 16 of 19 progeny (84%) from 14 nontreated infected dams harbored F hirthi worms.", "contents": "Transmission and control of Filaroides hirthi lungworm infection in dogs. Transmission of Filaroides hirthi among cagemate pups was demonstrated to occur through the ingestion of first-stage larvae in recently passed feces. It was suggested that coprophagia is the principal mechanism of transmission of F hirthi and that transmission from brood bitches to their litters occurs by this mechanism during and after the fourth or fifth week of the nursing period. Medication of 15 infected brood bitches with two courses of albendazole at a dosage rate of 50 mg/kg of body weight twice each day for 5 days resulted in complete absence of F hirthi infection among all 21 of their progeny, whereas 16 of 19 progeny (84%) from 14 nontreated infected dams harbored F hirthi worms."}
{"id": "PMID:475135", "title": "Free amino acids in the plasma and urine of dogs from birth to senescence.", "content": "Plasma and urinary amino acid concentrations were determined from a cross-sectional sample of dogs of all ages. Reference or base line values for these components were established for five different age groups. A general decrease in urinary excretion of amino acids for each gram of creatinine was found during the developmental period. This decrease was not correlated to changes in plasma concentrations, which varied for the individual amino acids. The decrease did appear to be related to the functional maturation of the kidney. Several methylated basic amino acids were identified in the urine. Of these, NG, NG- and NG,N'G-dimethylarginine, and trimethyllysine had not been previously reported as constituents of dig urine, but are major components of the urinary amino acids, expecially in newborn animals. The 3-methylhistidine is also excreted in large amounts by dogs and measurable quantities of this compound appear in the plasma. These findings suggest an active metabolic function for methylated amino acids in the dog.", "contents": "Free amino acids in the plasma and urine of dogs from birth to senescence. Plasma and urinary amino acid concentrations were determined from a cross-sectional sample of dogs of all ages. Reference or base line values for these components were established for five different age groups. A general decrease in urinary excretion of amino acids for each gram of creatinine was found during the developmental period. This decrease was not correlated to changes in plasma concentrations, which varied for the individual amino acids. The decrease did appear to be related to the functional maturation of the kidney. Several methylated basic amino acids were identified in the urine. Of these, NG, NG- and NG,N'G-dimethylarginine, and trimethyllysine had not been previously reported as constituents of dig urine, but are major components of the urinary amino acids, expecially in newborn animals. The 3-methylhistidine is also excreted in large amounts by dogs and measurable quantities of this compound appear in the plasma. These findings suggest an active metabolic function for methylated amino acids in the dog."}
{"id": "PMID:475136", "title": "Chronic active hepatitis in dogs associated with leptospires.", "content": "Chronic active hepatitis was diagnosed in five American Foxhounds from one kennel. Spirochetes were demonstrated in the liver in four of the dogs. A rising titer to Leptospira interrogans serovar grippotyphosa was found in the fifth dog, although spirochetes were not demonstrated in tissues. A serologic survey at the kennel revealed evidence of exposure of 6 of 13 dogs to grippotyphosa. This is the first report of an association between chronic active hepatitis leptospiral infection in any species.", "contents": "Chronic active hepatitis in dogs associated with leptospires. Chronic active hepatitis was diagnosed in five American Foxhounds from one kennel. Spirochetes were demonstrated in the liver in four of the dogs. A rising titer to Leptospira interrogans serovar grippotyphosa was found in the fifth dog, although spirochetes were not demonstrated in tissues. A serologic survey at the kennel revealed evidence of exposure of 6 of 13 dogs to grippotyphosa. This is the first report of an association between chronic active hepatitis leptospiral infection in any species."}
{"id": "PMID:475137", "title": "Uveoscleral flow of aqueous humor in the normal dog.", "content": "Fluorescein-labeled dextran and sodium fluorescein perfused in the posterior chamber of the normal dog eye egressed through the trabecular meshwork and intrascleral plexus, as well as posteriorly through the ciliary body into the suprachoroidal space and the sclera. Intraocular pressures of 20, 25, 30, and 60 mm of Hg did not produce detectable changes in the fluorescein-labeled dextran movement. Topical 1% atropine and 10% phenylephrine did not affect posterior passage of sodium fluorescein; 2% pilocarpine impeded posterior passage, causing an accumulation of sodium fluorescein and associated fluorescence in the anterior ciliary body.", "contents": "Uveoscleral flow of aqueous humor in the normal dog. Fluorescein-labeled dextran and sodium fluorescein perfused in the posterior chamber of the normal dog eye egressed through the trabecular meshwork and intrascleral plexus, as well as posteriorly through the ciliary body into the suprachoroidal space and the sclera. Intraocular pressures of 20, 25, 30, and 60 mm of Hg did not produce detectable changes in the fluorescein-labeled dextran movement. Topical 1% atropine and 10% phenylephrine did not affect posterior passage of sodium fluorescein; 2% pilocarpine impeded posterior passage, causing an accumulation of sodium fluorescein and associated fluorescence in the anterior ciliary body."}
{"id": "PMID:475138", "title": "Evaluation of data from mitogen studies in CBA mice: comparison of counts per minute, stimulation index and relative proliferation index.", "content": "Lymphocyte proliferation assays were conducted on splenic lymphocytes collected from CBA mice that had been exposed to lead acetate or cadmium chloride for 10 weeks. The ability of mitogens concanavalin A and lipopolysaccharide, as well as purified protein derivative, to induce proliferation in vitro in the presence or absence of macrophages was assessed. Data were compared by using counts per minute (cpm), stimulation index, and relative proliferative index. Data varied considerably within treatment groups performed on different days, which is a typical characteristic of mitogen proliferation investigations. According to cpm data, only 3 of 21 treatments were significantly (P less than 0.05) altered when compared with controls. The stimulation indices were highly influenced by background counts and frequently did not correlate with cpm. The relative proliferative index, on the other hand, calculates net cpm, closely corresponds to cpm, compensates for background counts and is affected less by variation than is the stimulation index, and selected cutoff values give a more sensitive measure for determination of altered proliferative responses when statistical procedures are not applicable. Effects of macrophages, carbon dioxide, and bovine fetal serum on lymphocyte transformation are discussed.", "contents": "Evaluation of data from mitogen studies in CBA mice: comparison of counts per minute, stimulation index and relative proliferation index. Lymphocyte proliferation assays were conducted on splenic lymphocytes collected from CBA mice that had been exposed to lead acetate or cadmium chloride for 10 weeks. The ability of mitogens concanavalin A and lipopolysaccharide, as well as purified protein derivative, to induce proliferation in vitro in the presence or absence of macrophages was assessed. Data were compared by using counts per minute (cpm), stimulation index, and relative proliferative index. Data varied considerably within treatment groups performed on different days, which is a typical characteristic of mitogen proliferation investigations. According to cpm data, only 3 of 21 treatments were significantly (P less than 0.05) altered when compared with controls. The stimulation indices were highly influenced by background counts and frequently did not correlate with cpm. The relative proliferative index, on the other hand, calculates net cpm, closely corresponds to cpm, compensates for background counts and is affected less by variation than is the stimulation index, and selected cutoff values give a more sensitive measure for determination of altered proliferative responses when statistical procedures are not applicable. Effects of macrophages, carbon dioxide, and bovine fetal serum on lymphocyte transformation are discussed."}
{"id": "PMID:475139", "title": "Age-related variations in cortical bone-remodeling measurements in male Beagles 10 to 26 months of age.", "content": "Age-related variations in bone-remodeling rates in the male Beagle were unknown. Variations were examined in 28 male Beagles whose ages were evenly distributed between 10 and 26 months. Results were analyzed by computer and regression lines for a best fit line for each determination were given; the 95% confidence limit for the best-fit line and the 95% confidence limits for the individual measurements were calculated. The results are graphically presented in this paper. There was a slight increase in total cortical area, although there was a decrease in the ratio of total-to-cortical area. Osteoid seams showed a frank decrease. Relatively little variation was found in circumference of osteoid seams and appositional rate. The number of resorption spaces decreased. There was a decrease in radial closure rate. Marked decreases were found in activation frequencies and bone formation rate. Increases were observed in osteon formation times and ratio of resorption to formation. The width of osteoid seams and the percentage of systems labeled decreased slightly. As a result of these experiments concerning variations in bone-remodeling activity carried out on Beagles (using the same dogs as their own controls), it was concluded that results obtained should be compared with the expected age-related activity of bone remodeling and not with the initial preexperimental biopsy results.", "contents": "Age-related variations in cortical bone-remodeling measurements in male Beagles 10 to 26 months of age. Age-related variations in bone-remodeling rates in the male Beagle were unknown. Variations were examined in 28 male Beagles whose ages were evenly distributed between 10 and 26 months. Results were analyzed by computer and regression lines for a best fit line for each determination were given; the 95% confidence limit for the best-fit line and the 95% confidence limits for the individual measurements were calculated. The results are graphically presented in this paper. There was a slight increase in total cortical area, although there was a decrease in the ratio of total-to-cortical area. Osteoid seams showed a frank decrease. Relatively little variation was found in circumference of osteoid seams and appositional rate. The number of resorption spaces decreased. There was a decrease in radial closure rate. Marked decreases were found in activation frequencies and bone formation rate. Increases were observed in osteon formation times and ratio of resorption to formation. The width of osteoid seams and the percentage of systems labeled decreased slightly. As a result of these experiments concerning variations in bone-remodeling activity carried out on Beagles (using the same dogs as their own controls), it was concluded that results obtained should be compared with the expected age-related activity of bone remodeling and not with the initial preexperimental biopsy results."}
{"id": "PMID:475140", "title": "Intraluminal esophageal sphincter manometry in dogs immobilized with xylazine.", "content": "The pressure in the pharyngoesophageal and the gastroesophageal sphincters in six normal dogs immobilized with xylazine was studied, using a single side hole catheter continuously perfused with distilled water by a compressed air pressure pump. Mean maximal resting pressures of the pharyngoesophageal and the gastroesophageal sphincters were 6 +/- 1.2 mm of Hg and 11 +/- 2.9 mm of Hg, respectively. The technique and results will enable better definition of esophageal sphincter abnormalities.", "contents": "Intraluminal esophageal sphincter manometry in dogs immobilized with xylazine. The pressure in the pharyngoesophageal and the gastroesophageal sphincters in six normal dogs immobilized with xylazine was studied, using a single side hole catheter continuously perfused with distilled water by a compressed air pressure pump. Mean maximal resting pressures of the pharyngoesophageal and the gastroesophageal sphincters were 6 +/- 1.2 mm of Hg and 11 +/- 2.9 mm of Hg, respectively. The technique and results will enable better definition of esophageal sphincter abnormalities."}
{"id": "PMID:475141", "title": "Echocardiography in the domestic cat.", "content": "Echocardiographic examinations were performed on 25 normal healthy cats placed in left lateral recumbency. The cats were representative of serveral breeds, consisted of 12 males and 13 females, and weighed between 2 and 6.5 kg. Twelve measurements from the echocardiograms of each cat were made for the purpose of compiling a small data base (reference values). These measurements included wall thicknesses, luminal dimensions, and contractile indices. Left ventricular wall thickness averaged 0.37 +/- 0.08 cm, and the septal wall thickness averaged 0.45 +/- 0.09 cm. The average left ventricular lumen in diastole was 1.48 +/- 0.26 cm, and in systole, it was 0.88 +/- 0.24 cm, which resulted in an average shortening fraction of 41 +/- 7.3%. Velocity of circumferential shortening, an index of contractility, averaged 2.86 +/- 0.78 cm. In eight additional cats, both ventricles were catheterized for echographic chamber identification and then cineangiographic procedures. Then these cats were euthanatized, and postmortem measurements were compared with echocardiographic and cineangiographic measurements. According to the three methods of determining wall thickness, differences were less than 15%, where the range of deviations was between 0 and 0.5 mm and the average deviation was -0.01 +/- 0.26 mm.", "contents": "Echocardiography in the domestic cat. Echocardiographic examinations were performed on 25 normal healthy cats placed in left lateral recumbency. The cats were representative of serveral breeds, consisted of 12 males and 13 females, and weighed between 2 and 6.5 kg. Twelve measurements from the echocardiograms of each cat were made for the purpose of compiling a small data base (reference values). These measurements included wall thicknesses, luminal dimensions, and contractile indices. Left ventricular wall thickness averaged 0.37 +/- 0.08 cm, and the septal wall thickness averaged 0.45 +/- 0.09 cm. The average left ventricular lumen in diastole was 1.48 +/- 0.26 cm, and in systole, it was 0.88 +/- 0.24 cm, which resulted in an average shortening fraction of 41 +/- 7.3%. Velocity of circumferential shortening, an index of contractility, averaged 2.86 +/- 0.78 cm. In eight additional cats, both ventricles were catheterized for echographic chamber identification and then cineangiographic procedures. Then these cats were euthanatized, and postmortem measurements were compared with echocardiographic and cineangiographic measurements. According to the three methods of determining wall thickness, differences were less than 15%, where the range of deviations was between 0 and 0.5 mm and the average deviation was -0.01 +/- 0.26 mm."}
{"id": "PMID:475142", "title": "Molar absorbance of cyanmethemoglobin from blood of different animals.", "content": "The molar absorbance of cyanmethemoglobin at 540 nm was measured in 35 species of mammals, birds, and reptiles. Values obtained were compared with those measured on human cyanmethemoglobin by the same procedure. There was no significant difference (P greater than or equal to 0.1) between the human and animal blood values observed in 29 samples. In six cases (4 mammals, 1 reptile, and 1 bird), the significant difference (P greater than or equal to 0.05) did not exceed 5% of the human value. The practice of applying the accepted absorbance of 1.10 x 10(4) M-1 cm-1 per mole of iron in human cyanmethemeglobin for the analysis of animal blood will not cause more than 5% uncertainty. Such results are satisfactory for use in clinical veterinary medicine except for those research projects concerned with animal hemoglobin function and structure.", "contents": "Molar absorbance of cyanmethemoglobin from blood of different animals. The molar absorbance of cyanmethemoglobin at 540 nm was measured in 35 species of mammals, birds, and reptiles. Values obtained were compared with those measured on human cyanmethemoglobin by the same procedure. There was no significant difference (P greater than or equal to 0.1) between the human and animal blood values observed in 29 samples. In six cases (4 mammals, 1 reptile, and 1 bird), the significant difference (P greater than or equal to 0.05) did not exceed 5% of the human value. The practice of applying the accepted absorbance of 1.10 x 10(4) M-1 cm-1 per mole of iron in human cyanmethemeglobin for the analysis of animal blood will not cause more than 5% uncertainty. Such results are satisfactory for use in clinical veterinary medicine except for those research projects concerned with animal hemoglobin function and structure."}
{"id": "PMID:475144", "title": "Pharmacokinetics of gentamicin in blood plasma of quail, pheasants, and cranes.", "content": "Rate of appearance, peak concentration, and the biological half-life of gentamicin in the plasma of quail (Coturnix coturnix), pheasants (Phasianus colchicus), and cranes (Grus canadensis tabida) were studied. Gentamicin was given IM in doses of 5, 10, and 20 mg/kg of body weight. Peak plasma concentrations occurred earliest in the quail, latest in the cranes. The peak concentrations varied directly with the administered doses in all species. The biological half-life of gentamicin was 42 +/- 12 minutes in the quail, 75 +/- 15 minutes in the pheasants, and 165 +/- 37 minutes in the cranes. On the basis of the present data, dosage regimens for gentaminic of 5 mg/kg every 8 hours in pheasants and cranes, and 10 mg/kg every 6 hours in quail, would be expected to give constant plasma concentrations greater than 4.0 micrograms/ml.", "contents": "Pharmacokinetics of gentamicin in blood plasma of quail, pheasants, and cranes. Rate of appearance, peak concentration, and the biological half-life of gentamicin in the plasma of quail (Coturnix coturnix), pheasants (Phasianus colchicus), and cranes (Grus canadensis tabida) were studied. Gentamicin was given IM in doses of 5, 10, and 20 mg/kg of body weight. Peak plasma concentrations occurred earliest in the quail, latest in the cranes. The peak concentrations varied directly with the administered doses in all species. The biological half-life of gentamicin was 42 +/- 12 minutes in the quail, 75 +/- 15 minutes in the pheasants, and 165 +/- 37 minutes in the cranes. On the basis of the present data, dosage regimens for gentaminic of 5 mg/kg every 8 hours in pheasants and cranes, and 10 mg/kg every 6 hours in quail, would be expected to give constant plasma concentrations greater than 4.0 micrograms/ml."}
{"id": "PMID:475151", "title": "A four-year follow-up study of lung mechanics in smokers.", "content": "We performed a prospective study of pulmonary function in 21 smokers, 9 ex-smokers, and 12 nonsmokers. The smokers and ex-smokers were preselected, because they were participants in a smoking cessation clinic. An average interval of 4 years separated the first and second (follow-up) studies. The smoking group showed a significant decrease in maximal expiratory flow measured at low lung volume, loss of elastic recoil, increase in lung compliance, increase in total lung capacity; increase in the ratio of residual volume to total lung capacity, and an increase in the ratio of functional residual capacity to total lung capacity. The ex-smokers showed changes similar to those of the smokers, but of lesser magnitude. The nonsmoking group demonstrated few changes in function during the study interval. Commonly measured parameters of function, including the ratio of the forced expiratory volume in 1 sec to the forced vital capacity and the maximal expiratory flow after exhalation of 50 per cent of the vital capacity, did not change significantly in any group. Sensitive tests of lung function were abnormal in a very high percentage of the combined group of smokers and ex-smokers when measured at the time of the second study; only a small number of abnormalities in these parameters were noted in the nonsmoking group. We conclude that there was a deterioration of lung function in smokers far in excess of that predicted by age. These changes suggest the development of emphysema and were predictable for the group as a whole by a high prevalence of abnormality of dynamic compliance, closing volume, maximal mid-expiratory flow, and residual volume at the time of the initial study.", "contents": "A four-year follow-up study of lung mechanics in smokers. We performed a prospective study of pulmonary function in 21 smokers, 9 ex-smokers, and 12 nonsmokers. The smokers and ex-smokers were preselected, because they were participants in a smoking cessation clinic. An average interval of 4 years separated the first and second (follow-up) studies. The smoking group showed a significant decrease in maximal expiratory flow measured at low lung volume, loss of elastic recoil, increase in lung compliance, increase in total lung capacity; increase in the ratio of residual volume to total lung capacity, and an increase in the ratio of functional residual capacity to total lung capacity. The ex-smokers showed changes similar to those of the smokers, but of lesser magnitude. The nonsmoking group demonstrated few changes in function during the study interval. Commonly measured parameters of function, including the ratio of the forced expiratory volume in 1 sec to the forced vital capacity and the maximal expiratory flow after exhalation of 50 per cent of the vital capacity, did not change significantly in any group. Sensitive tests of lung function were abnormal in a very high percentage of the combined group of smokers and ex-smokers when measured at the time of the second study; only a small number of abnormalities in these parameters were noted in the nonsmoking group. We conclude that there was a deterioration of lung function in smokers far in excess of that predicted by age. These changes suggest the development of emphysema and were predictable for the group as a whole by a high prevalence of abnormality of dynamic compliance, closing volume, maximal mid-expiratory flow, and residual volume at the time of the initial study."}
{"id": "PMID:475152", "title": "Relationship between the single-breath N test and age, sex, and smoking habit in three North American cities.", "content": "This report describes a collaborative study conducted in Montreal, Canada, Portland, Ore., and Winnipeg, Canada, to establish the relationship between the single-breath N2 test and age, sex, and smoking and to determine the prevalence of functional abnormalities in these populations. In nonsmokers, age-related regressions for closing volume, closing capacity, and the slope of phase III obtained from the single-breath N2 test, plus the ratio of the I-s forced expiratory volume to the forced vital capacity had very similar slopes, suggesting that differences in geographic location, climate, air pollution, and occupation had no effect on lung function detectable by these tests. Among the 6 city/six groups there was no systematic difference in the prevalence of functional abnormalities between the cities, but closing capacity expressed as a percentage of total lung capacity was abnormal most often in men and the slope of the alveolar plateau was abnormal most often in women. The prevalence of respiratory symptoms within different smoking categories was similar in the 3 cities. Although the number of cigarettes smoked had a significant effect on every test except the ratio of the I-s forced expiratory volume to forced vital capacity in men, the effect of age was considerably greater than the effect of smoking, and the dose-response relationship was weak. We conclude that additional factors may interact with smoking to place a smoker at risk of developing chronic airflow limitation.", "contents": "Relationship between the single-breath N test and age, sex, and smoking habit in three North American cities. This report describes a collaborative study conducted in Montreal, Canada, Portland, Ore., and Winnipeg, Canada, to establish the relationship between the single-breath N2 test and age, sex, and smoking and to determine the prevalence of functional abnormalities in these populations. In nonsmokers, age-related regressions for closing volume, closing capacity, and the slope of phase III obtained from the single-breath N2 test, plus the ratio of the I-s forced expiratory volume to the forced vital capacity had very similar slopes, suggesting that differences in geographic location, climate, air pollution, and occupation had no effect on lung function detectable by these tests. Among the 6 city/six groups there was no systematic difference in the prevalence of functional abnormalities between the cities, but closing capacity expressed as a percentage of total lung capacity was abnormal most often in men and the slope of the alveolar plateau was abnormal most often in women. The prevalence of respiratory symptoms within different smoking categories was similar in the 3 cities. Although the number of cigarettes smoked had a significant effect on every test except the ratio of the I-s forced expiratory volume to forced vital capacity in men, the effect of age was considerably greater than the effect of smoking, and the dose-response relationship was weak. We conclude that additional factors may interact with smoking to place a smoker at risk of developing chronic airflow limitation."}
{"id": "PMID:475153", "title": "Controlled exposures of human volunteers to sulfate aerosols. Health effects and aerosol characterization.", "content": "Our laboratory has undertaken the study of possible acute adverse health effects of sulfate aerosols through controlled exposures of volunteer human subjects. Both healthy and asthmatic adult men were exposed for 2-hour periods (with intermittent exercise) to ammonium sulfate, ammonium bisulfate, and sulfuric acid of particle size distributions and concentrations intended to simulate \"worst case\" exposures during Los Angeles smog episodes. Lung function tests were performed by the subjects on entering and before exiting from a carefully controlled environmental chamber. Subject symptoms were evluated in a standardized manner. Aerosol concentrations and size distributions were determined by an on-line computer/aerometric monitoring  system; gravimetric and chemical analyses were performed on impactor and total filter samples after test exposures. We found little or no evidence of adverse health effects from 2-hour multiple-day exposures to any of the compounds at \"worst case\" ambient concentrations.", "contents": "Controlled exposures of human volunteers to sulfate aerosols. Health effects and aerosol characterization. Our laboratory has undertaken the study of possible acute adverse health effects of sulfate aerosols through controlled exposures of volunteer human subjects. Both healthy and asthmatic adult men were exposed for 2-hour periods (with intermittent exercise) to ammonium sulfate, ammonium bisulfate, and sulfuric acid of particle size distributions and concentrations intended to simulate \"worst case\" exposures during Los Angeles smog episodes. Lung function tests were performed by the subjects on entering and before exiting from a carefully controlled environmental chamber. Subject symptoms were evluated in a standardized manner. Aerosol concentrations and size distributions were determined by an on-line computer/aerometric monitoring  system; gravimetric and chemical analyses were performed on impactor and total filter samples after test exposures. We found little or no evidence of adverse health effects from 2-hour multiple-day exposures to any of the compounds at \"worst case\" ambient concentrations."}
{"id": "PMID:475154", "title": "Metronidazole in the treatment of anaerobic infections.", "content": "Using metronidazole in oral dosages of 1.5 to 2 g daily, we treated 13 adults who had anaerobic pleuropulmonary infections, including 11 with lung abscess, one with necrotizing pneumonia, and one with thoracic empyema. Five patients (four with lung abscess and one with necrotizing pneumonia) were cured. The lung abscesses of 5 patients did not respond. For 3 patients (one with epigastric distress who refused metronidazole, one with undrained empyema, and one who died while receiving metronidazole), therapy could not be evaluated. Side effects included leukopenia (2 patients), leukopenia and neutropenia (one), neutropenia (one), dark urine (two), bitter taste (two), and epigastric distress (one). In light of our findings, metronidazole is not uniformly effective in the treatment of anaerobic pleuropulmonary infections.", "contents": "Metronidazole in the treatment of anaerobic infections. Using metronidazole in oral dosages of 1.5 to 2 g daily, we treated 13 adults who had anaerobic pleuropulmonary infections, including 11 with lung abscess, one with necrotizing pneumonia, and one with thoracic empyema. Five patients (four with lung abscess and one with necrotizing pneumonia) were cured. The lung abscesses of 5 patients did not respond. For 3 patients (one with epigastric distress who refused metronidazole, one with undrained empyema, and one who died while receiving metronidazole), therapy could not be evaluated. Side effects included leukopenia (2 patients), leukopenia and neutropenia (one), neutropenia (one), dark urine (two), bitter taste (two), and epigastric distress (one). In light of our findings, metronidazole is not uniformly effective in the treatment of anaerobic pleuropulmonary infections."}
{"id": "PMID:475155", "title": "Influence of dose and fiber type on respiratory malignancy risk in asbestos cement manufacturing.", "content": "This investigation provides information concerning the risk or respiratory malignancy in relation to duration, degree, and fiber type of exposure to asbestos in a manufacturing cohort of 5,645 with long-term follow-up. Excess mortality for this cause was found in groups with moderate and high cumulative exposure (standard mortality ratios of 290 and 226). Analysis of the influence of components of total exposure dose (duration, average concentration) revealed no detectable excess risk in persons employed for less than 2 years or with low-degree average exposure. Exposure to corcidolite (blue) fiber in addition to the predominantly used chrysotile in pipe making appeared to be associated with higher risk than was exposure to chrysotile alone.", "contents": "Influence of dose and fiber type on respiratory malignancy risk in asbestos cement manufacturing. This investigation provides information concerning the risk or respiratory malignancy in relation to duration, degree, and fiber type of exposure to asbestos in a manufacturing cohort of 5,645 with long-term follow-up. Excess mortality for this cause was found in groups with moderate and high cumulative exposure (standard mortality ratios of 290 and 226). Analysis of the influence of components of total exposure dose (duration, average concentration) revealed no detectable excess risk in persons employed for less than 2 years or with low-degree average exposure. Exposure to corcidolite (blue) fiber in addition to the predominantly used chrysotile in pipe making appeared to be associated with higher risk than was exposure to chrysotile alone."}
{"id": "PMID:475157", "title": "The effects of sodium substitution and ouabain on ion transport by dog tracheal epithelium.", "content": "Using ouabain and sodium removal, we studied the mechanism of chloride transport by dog tracheal epithelium. When applied to the submucosal side of the tissue, 2 x 10(-4) M ouabain decreased the short-circuit current, the net chloride flux toward the lumen, and the net sodium flux toward the submucosa to zero after 30 min. Applied to the luminal side, ouabain had little effect. Potassium-free medium, like ouabain, decreased the short-circuit current when present in the submucosal bath; on the luminal side ti produced a slight increase (7.0 +/- 1.5 per cent; n = 6) in short-circuit current. Replacement of sodium in the submucosal bathing medium by choline led to a decrease in net chloride flux of 84 per cent. Sodium replacement in the luminal bath produced no change in net chloride flux. It is proposed that chloride secretion by this tissue depends on active basolateral sodium pumps, and that a component of chloride entry into the transporting cells from the submucosal medium may be sodium-linked.", "contents": "The effects of sodium substitution and ouabain on ion transport by dog tracheal epithelium. Using ouabain and sodium removal, we studied the mechanism of chloride transport by dog tracheal epithelium. When applied to the submucosal side of the tissue, 2 x 10(-4) M ouabain decreased the short-circuit current, the net chloride flux toward the lumen, and the net sodium flux toward the submucosa to zero after 30 min. Applied to the luminal side, ouabain had little effect. Potassium-free medium, like ouabain, decreased the short-circuit current when present in the submucosal bath; on the luminal side ti produced a slight increase (7.0 +/- 1.5 per cent; n = 6) in short-circuit current. Replacement of sodium in the submucosal bathing medium by choline led to a decrease in net chloride flux of 84 per cent. Sodium replacement in the luminal bath produced no change in net chloride flux. It is proposed that chloride secretion by this tissue depends on active basolateral sodium pumps, and that a component of chloride entry into the transporting cells from the submucosal medium may be sodium-linked."}
{"id": "PMID:475159", "title": "Alveolar-capillary membrane permeability. Correlation with functional, radiographic, and postmortem changes after fluid aspiration.", "content": "An index of permeability of the alveolar-capillary membrane was derived from the relative extraction from the lung into arterial blood of 2 tracers, 125I-antipyrine and 51Cr-ethylenediaminetetraacetate. The effect on this index of aspirating 2 ml of isotonic saline, distilled water, or 10(-1) M hydrochloric acid per kg of body weight was studied in 3 groups of rabbits. The severity and time course of changes in the permeability index were correlated with changes in lung mechanics, gas exchange, serial chest roentgenograms, ratio of extravascular lung water to dry weight, and histologic findings. Aspiration of saline produced no change in the permeability index; aspiration of water produced a large mean +/- SEM increase in the index, from a baseline of 0.025 +/- 0.002 to 1,050 +/- 0.054 (P less than 0.001), but this value returned to baseline 15 min later. After hydrochloric acid, the permeability index increased from a baseline of 0.027 +/- 0.003 to 1.068 +/- 0.098 (P less than 0.005), with no evidence of resolution after 60 min. Changes in lung mechanics, gas exchange, and roentgenograms were smallest after aspiration of distilled water and greatest after aspiration of hydrochloric acid. The functional changes after aspiration of water and saline recovered at a rate proportional to the known clearance rates of these liquids from the lung. The changes after hydrochloric acid either showed no tendency toward recovery or, in the case of the roentgenograms, worsened with time. There were no detectable histologic abnormalities or an increase in the ratio of extravascular lung water to dry weight after aspiration of water or saline, but there were extensive histologic abnormalities and a 70 per cent increase in lung water after acid. The increase in the permeability index after aspiration of water was too transient to exert a deleterious effect. In contrast, the persistence of the increase in the permeability index after hydrochloric acid was associated with persistent functional changes attributable to the considerable increase in lung water.", "contents": "Alveolar-capillary membrane permeability. Correlation with functional, radiographic, and postmortem changes after fluid aspiration. An index of permeability of the alveolar-capillary membrane was derived from the relative extraction from the lung into arterial blood of 2 tracers, 125I-antipyrine and 51Cr-ethylenediaminetetraacetate. The effect on this index of aspirating 2 ml of isotonic saline, distilled water, or 10(-1) M hydrochloric acid per kg of body weight was studied in 3 groups of rabbits. The severity and time course of changes in the permeability index were correlated with changes in lung mechanics, gas exchange, serial chest roentgenograms, ratio of extravascular lung water to dry weight, and histologic findings. Aspiration of saline produced no change in the permeability index; aspiration of water produced a large mean +/- SEM increase in the index, from a baseline of 0.025 +/- 0.002 to 1,050 +/- 0.054 (P less than 0.001), but this value returned to baseline 15 min later. After hydrochloric acid, the permeability index increased from a baseline of 0.027 +/- 0.003 to 1.068 +/- 0.098 (P less than 0.005), with no evidence of resolution after 60 min. Changes in lung mechanics, gas exchange, and roentgenograms were smallest after aspiration of distilled water and greatest after aspiration of hydrochloric acid. The functional changes after aspiration of water and saline recovered at a rate proportional to the known clearance rates of these liquids from the lung. The changes after hydrochloric acid either showed no tendency toward recovery or, in the case of the roentgenograms, worsened with time. There were no detectable histologic abnormalities or an increase in the ratio of extravascular lung water to dry weight after aspiration of water or saline, but there were extensive histologic abnormalities and a 70 per cent increase in lung water after acid. The increase in the permeability index after aspiration of water was too transient to exert a deleterious effect. In contrast, the persistence of the increase in the permeability index after hydrochloric acid was associated with persistent functional changes attributable to the considerable increase in lung water."}
{"id": "PMID:475160", "title": "Ultrasonographic evaluation of ventilatory effect on inferior vena caval configuration.", "content": "Ventilatory effect on inferior vena caval configuration was studied by ultrasonography in 14 subjects, including 5 with chronic obstructive pulmonary disease, 3 with cardiac tamponade due to carcinomatous pericardial effusion, one with tuberculous constrictive pericarditis, and 5 normal subjects. The inferior vena caval lumen decreased in the early inspiratory phase, reached a minimum at the end of inspiration, distended again during expiration, and closed transiently 2 to 3 cm below the diaphragm during maximal inspiration. These ventilatory changes of the inferior vena cava reversed with increase in intrathoracic pressure during the Valsalva maneuver and positive-pressure ventilation. When the central venous pressure was increased, as in cardiac tamponade, the inferior vena cava was fully distended through the entire phase of inspiration and expiration. Collapsibility of the inferior vena cava was inversely proportional to central venous pressure when the pressure was less than 10 cm H2O, but not when the pressure was greater than approximately 10 cm H2O. Our results suggest that the study of inferior vena caval configuration with ultrasonogrphy is a valuable noninvasive clinical aid for estimating central venous pressure and for analyzing inferior vena caval hemodynamics in various clinical conditions.", "contents": "Ultrasonographic evaluation of ventilatory effect on inferior vena caval configuration. Ventilatory effect on inferior vena caval configuration was studied by ultrasonography in 14 subjects, including 5 with chronic obstructive pulmonary disease, 3 with cardiac tamponade due to carcinomatous pericardial effusion, one with tuberculous constrictive pericarditis, and 5 normal subjects. The inferior vena caval lumen decreased in the early inspiratory phase, reached a minimum at the end of inspiration, distended again during expiration, and closed transiently 2 to 3 cm below the diaphragm during maximal inspiration. These ventilatory changes of the inferior vena cava reversed with increase in intrathoracic pressure during the Valsalva maneuver and positive-pressure ventilation. When the central venous pressure was increased, as in cardiac tamponade, the inferior vena cava was fully distended through the entire phase of inspiration and expiration. Collapsibility of the inferior vena cava was inversely proportional to central venous pressure when the pressure was less than 10 cm H2O, but not when the pressure was greater than approximately 10 cm H2O. Our results suggest that the study of inferior vena caval configuration with ultrasonogrphy is a valuable noninvasive clinical aid for estimating central venous pressure and for analyzing inferior vena caval hemodynamics in various clinical conditions."}
{"id": "PMID:475164", "title": "Attenuation of prazosin effect on cardiac output in chronic heart failure.", "content": "The persistence of the hemodynamic effects of prazosin was studied in 12 patients with chronic congestive heart failure. Multidose evaluation involving five 5-mg doses showed the initial decrease in systemic vascular resistance and increase in cardiac index, stroke work index, and stroke volume index to be transient. Doubling the dose did not restore effect. Modest decreases in pulmonary capillary-wedge and mean arterial pressures persisted throughout the study. In six patients, plasma prazosin concentration measured at times of hemodynamic observations showed the initial hemodynamic effect of prazosin to attenuate upon further administration despite mean plasma concentrations that exceeded those measured after the first dose. In patients with chronic heart failure, resting hemodynamic studies suggest a rapid attenuation of prazosin-mediated hemodynamic effect in the presence of adequate plasma concentration. Recognizing this phenomenon, if long-term prazosin therapy for congestive heart failure is contemplated, we suggest the hemodynamic response in individual patients be monitored.", "contents": "Attenuation of prazosin effect on cardiac output in chronic heart failure. The persistence of the hemodynamic effects of prazosin was studied in 12 patients with chronic congestive heart failure. Multidose evaluation involving five 5-mg doses showed the initial decrease in systemic vascular resistance and increase in cardiac index, stroke work index, and stroke volume index to be transient. Doubling the dose did not restore effect. Modest decreases in pulmonary capillary-wedge and mean arterial pressures persisted throughout the study. In six patients, plasma prazosin concentration measured at times of hemodynamic observations showed the initial hemodynamic effect of prazosin to attenuate upon further administration despite mean plasma concentrations that exceeded those measured after the first dose. In patients with chronic heart failure, resting hemodynamic studies suggest a rapid attenuation of prazosin-mediated hemodynamic effect in the presence of adequate plasma concentration. Recognizing this phenomenon, if long-term prazosin therapy for congestive heart failure is contemplated, we suggest the hemodynamic response in individual patients be monitored."}
{"id": "PMID:475165", "title": "Coronary artery narrowing in coronary heart disease: comparison of cineangiographic and necropsy findings.", "content": "Of 10 patients with fatal coronary heart disease undergoing coronary angiography 0 to 69 d (average, 21) before necropsy, the amount of narrowing in 61 coronary arteries observed angiographically (diameter reduction) during life by three angiographers was compared with that observed histologically (cross-sectional area) at necropsy. No overestimations of the degree of narrowing were made angiographically. Of 11 coronary arteries or their subdivisions narrowed 0 to 50% in cross-sectional area histologically, none were underestimated angiographically; of eight narrowed 51% to 75% histologically, seven had been underestimated, and of 42 narrowed 76% to 100% histologically, 17 were underestimated angiographically. The coronary atherosclerotic plaquing was diffuse (greater than 25% cross-sectional area narrowing) in 90% of 467 five-millimetre segments of coronary artery examined (24 cm per patient), and this diffuseness of the atherosclerotic process seems to be the major reason for angiographic underestimation of coronary narrowings.", "contents": "Coronary artery narrowing in coronary heart disease: comparison of cineangiographic and necropsy findings. Of 10 patients with fatal coronary heart disease undergoing coronary angiography 0 to 69 d (average, 21) before necropsy, the amount of narrowing in 61 coronary arteries observed angiographically (diameter reduction) during life by three angiographers was compared with that observed histologically (cross-sectional area) at necropsy. No overestimations of the degree of narrowing were made angiographically. Of 11 coronary arteries or their subdivisions narrowed 0 to 50% in cross-sectional area histologically, none were underestimated angiographically; of eight narrowed 51% to 75% histologically, seven had been underestimated, and of 42 narrowed 76% to 100% histologically, 17 were underestimated angiographically. The coronary atherosclerotic plaquing was diffuse (greater than 25% cross-sectional area narrowing) in 90% of 467 five-millimetre segments of coronary artery examined (24 cm per patient), and this diffuseness of the atherosclerotic process seems to be the major reason for angiographic underestimation of coronary narrowings."}
{"id": "PMID:475166", "title": "Reproducibility of hemoglobin AIc and sensitivity to various degrees of glucose intolerance.", "content": "To establish a normal range of hemoglobin (Hb) AIc, and ascertain the sensitivity and reproducibility of this test, three groups were studied: patients with suspected diabetes undergoing a standard oral glucose tolerance test, diabetic outpatients, and subjects with normal carbohydrate tolerance studies on two separate occasions. In Group 1, the mean Hb AIc (+/- SD) in 165 carbohydrate-tolerant subjects was 4.97 +/- 0.50% and the normal range was the mean +/- 2 standard deviations (4.0 to 6.0%). The mean Hb AIc (+/- SD) of the 63 carbohydrate-intolerant subjects was 6.45 +/- 1.14, significantly greater than the normal subjects. In both subgroups, Hb AIc correlated significantly with the fasting blood glucose but not with the glucose areas of the oral glucose-tolerance test. In Group 2, the Hb AIc values were normal in 39% of those diet treated, 10% of those on oral agents, and none of those on insulin therapy. In Group 3, the mean coefficient of variation of the two Hb AIc values for each subject was 6.86% but was 7.15% for the fasting blood glucose. These findings suggest that Hb AIc is highly reproducible and responsive to minor degrees of abnormality of glucose tolerance and may provide an alternative method for defining carbohydrate tolerance or the degree of blood sugar control.", "contents": "Reproducibility of hemoglobin AIc and sensitivity to various degrees of glucose intolerance. To establish a normal range of hemoglobin (Hb) AIc, and ascertain the sensitivity and reproducibility of this test, three groups were studied: patients with suspected diabetes undergoing a standard oral glucose tolerance test, diabetic outpatients, and subjects with normal carbohydrate tolerance studies on two separate occasions. In Group 1, the mean Hb AIc (+/- SD) in 165 carbohydrate-tolerant subjects was 4.97 +/- 0.50% and the normal range was the mean +/- 2 standard deviations (4.0 to 6.0%). The mean Hb AIc (+/- SD) of the 63 carbohydrate-intolerant subjects was 6.45 +/- 1.14, significantly greater than the normal subjects. In both subgroups, Hb AIc correlated significantly with the fasting blood glucose but not with the glucose areas of the oral glucose-tolerance test. In Group 2, the Hb AIc values were normal in 39% of those diet treated, 10% of those on oral agents, and none of those on insulin therapy. In Group 3, the mean coefficient of variation of the two Hb AIc values for each subject was 6.86% but was 7.15% for the fasting blood glucose. These findings suggest that Hb AIc is highly reproducible and responsive to minor degrees of abnormality of glucose tolerance and may provide an alternative method for defining carbohydrate tolerance or the degree of blood sugar control."}
{"id": "PMID:475167", "title": "Q fever endocarditis in the United States.", "content": "A patient with Q fever endocarditis, which is almost unknown in the United States, was followed for a total of 32 months; the study was begun 3 1/2 months before aortic valve replacement. Diagnosis was confirmed by serology, visualization of Coxiella burnetii in excised aortic valve tissue by direct and immunofluorescence staining, and isolation of C. burnetii from aortic valve tissue. Serum antibodies against phase I and phase II antigens of C. burnetii were identified. Almost all phase I and phase II antibodies were IgG. These findings are compared with those in an uncomplicated case of acute Q fever. New findings on the immune response to chronic Q fever are presented.", "contents": "Q fever endocarditis in the United States. A patient with Q fever endocarditis, which is almost unknown in the United States, was followed for a total of 32 months; the study was begun 3 1/2 months before aortic valve replacement. Diagnosis was confirmed by serology, visualization of Coxiella burnetii in excised aortic valve tissue by direct and immunofluorescence staining, and isolation of C. burnetii from aortic valve tissue. Serum antibodies against phase I and phase II antigens of C. burnetii were identified. Almost all phase I and phase II antibodies were IgG. These findings are compared with those in an uncomplicated case of acute Q fever. New findings on the immune response to chronic Q fever are presented."}
{"id": "PMID:475168", "title": "Low-pressure cardiac tamponade.", "content": "An elderly man developed cardiac tamponade from a tuberculous pericardial effusion but without such typical manifestations as pulsus paradoxus and jugular-vein distension. This case illustrates the difficulties in clinical recognition of low-pressure cardiac tamponade, which can develop in the presence of dehydration and hypovolemia. The hemodynamic factors that account for this phenomenon are discussed.", "contents": "Low-pressure cardiac tamponade. An elderly man developed cardiac tamponade from a tuberculous pericardial effusion but without such typical manifestations as pulsus paradoxus and jugular-vein distension. This case illustrates the difficulties in clinical recognition of low-pressure cardiac tamponade, which can develop in the presence of dehydration and hypovolemia. The hemodynamic factors that account for this phenomenon are discussed."}
{"id": "PMID:475169", "title": "Transient thyrotoxicosis associated with acute hemorrhagic infarction of autonomously functioning thyroid nodules.", "content": "Two patients with nontoxic autonomously functioning thyroid nodules had transient hyperthyroidism after acute hemorrhagic infarction. Previously solid \"hot\" nodules were converted to cystic \"cold\" nodules. Aspiration confirmed the hemorrhagic nature of the process. There was marked regression in nodule size, marked reduction of nodular autonomous secretory activity (indicated by a change from a blunted to a normal response to thyrotropin-releasing hormone), and spontaneous recovery of function in the previously suppressed extranodular thyroid tissue. Confusion of this entity with a nontoxic autonomously functioning thyroid nodule that has progressed to the toxic stage may be avoided if the loss of nodular function is noted on imaging. The possibility of granulomatous thyroiditis may be excluded by blood aspiration.", "contents": "Transient thyrotoxicosis associated with acute hemorrhagic infarction of autonomously functioning thyroid nodules. Two patients with nontoxic autonomously functioning thyroid nodules had transient hyperthyroidism after acute hemorrhagic infarction. Previously solid \"hot\" nodules were converted to cystic \"cold\" nodules. Aspiration confirmed the hemorrhagic nature of the process. There was marked regression in nodule size, marked reduction of nodular autonomous secretory activity (indicated by a change from a blunted to a normal response to thyrotropin-releasing hormone), and spontaneous recovery of function in the previously suppressed extranodular thyroid tissue. Confusion of this entity with a nontoxic autonomously functioning thyroid nodule that has progressed to the toxic stage may be avoided if the loss of nodular function is noted on imaging. The possibility of granulomatous thyroiditis may be excluded by blood aspiration."}
{"id": "PMID:475170", "title": "Coccidioidomycosis of the thyroid.", "content": "In two patients with thyroiditis caused by Coccidioides immitis, presented thyroid symptoms and findings were characteristic of subacute thyroiditis. Fine needle aspiration biopsy of the thyroid proved useful in establishing the diagnosis of coccidioidomycosis. This is the first report of coccidioidomycosis presenting as thyroiditis.", "contents": "Coccidioidomycosis of the thyroid. In two patients with thyroiditis caused by Coccidioides immitis, presented thyroid symptoms and findings were characteristic of subacute thyroiditis. Fine needle aspiration biopsy of the thyroid proved useful in establishing the diagnosis of coccidioidomycosis. This is the first report of coccidioidomycosis presenting as thyroiditis."}
{"id": "PMID:475176", "title": "How cardiologists learn about echocardiography. A reminder for medical educators and legislators.", "content": "Mandatory continuing medical education, because of the need to validate participation, rewards classroom activities but not self-education. To determine if self-education is still a major study method for practicing physicians, we surveyed 158 cardiologists to learn how they first heard of, and continued their education in, echocardiography, a technique in which 81% of the physician-sample receiving no training in medical school, residency, or fellowship. Initial and continuing sources of information included professional journals and literature, meetings and conferences, discussion with colleagues, and courses. Professional journals ranked first in use; individual and group learning activities were used about equally by physicians. Recent legislation requiring validation of attendance may cause educators and legislators to ignore the self-learner. This study should remind educators and legislators that variations in learning style must be considered when planning and legislating approaches in continuing medical education.", "contents": "How cardiologists learn about echocardiography. A reminder for medical educators and legislators. Mandatory continuing medical education, because of the need to validate participation, rewards classroom activities but not self-education. To determine if self-education is still a major study method for practicing physicians, we surveyed 158 cardiologists to learn how they first heard of, and continued their education in, echocardiography, a technique in which 81% of the physician-sample receiving no training in medical school, residency, or fellowship. Initial and continuing sources of information included professional journals and literature, meetings and conferences, discussion with colleagues, and courses. Professional journals ranked first in use; individual and group learning activities were used about equally by physicians. Recent legislation requiring validation of attendance may cause educators and legislators to ignore the self-learner. This study should remind educators and legislators that variations in learning style must be considered when planning and legislating approaches in continuing medical education."}
{"id": "PMID:475206", "title": "[Conduction abnormalities after single heart valve replacement (author's transl)].", "content": "A total of 300 patients were operated upon for heart valve disease by the same surgeon. The affected valve was the aortic in 200 cases and the mitral in the other 100 patients. The tricuspid valve, coronary arteries, and myocardium were not involved. This report attempts to define the frequency progression, prognosis, and significance of conduction abnormalities which appear during the course of this operation. In 35 cases, of which 16 were post-operative deaths, a histological examination of the conduction pathways was possible. The conclusions to be drawn from these observations are: Conduction abnormalities after single aortic or mitral valve replacement are frequent (1 out of 3 patients).", "contents": "[Conduction abnormalities after single heart valve replacement (author's transl)]. A total of 300 patients were operated upon for heart valve disease by the same surgeon. The affected valve was the aortic in 200 cases and the mitral in the other 100 patients. The tricuspid valve, coronary arteries, and myocardium were not involved. This report attempts to define the frequency progression, prognosis, and significance of conduction abnormalities which appear during the course of this operation. In 35 cases, of which 16 were post-operative deaths, a histological examination of the conduction pathways was possible. The conclusions to be drawn from these observations are: Conduction abnormalities after single aortic or mitral valve replacement are frequent (1 out of 3 patients)."}
{"id": "PMID:475207", "title": "[Use of urokinase during surgery: a new approach to the treatment of vascular obliterative disease (author's transl)].", "content": "Thrombolysis occurred in five out of eleven patients treated with intra-arterial urokinase for recent raterial obliteration. These positive results were accompanied by perfect tolerance and absence of systemic fibrinolysis. In some cases, therefore, surgical revascularization can be associated with fibrinolytic treatment.", "contents": "[Use of urokinase during surgery: a new approach to the treatment of vascular obliterative disease (author's transl)]. Thrombolysis occurred in five out of eleven patients treated with intra-arterial urokinase for recent raterial obliteration. These positive results were accompanied by perfect tolerance and absence of systemic fibrinolysis. In some cases, therefore, surgical revascularization can be associated with fibrinolytic treatment."}
{"id": "PMID:475208", "title": "[Foot lesions in non-insulin-dependent diabetes. A report on 25 cases (author's transl)].", "content": "Diabetic lesions in 25 patients with maturity-onset non-insulin-dependent diabetes, but with neuropathy and even other complications in spite of previous treatment, were studied. Distribution according to the duration of the diabetes and the age of the patients was comparable to that seen in insulin-dependent diabetes. The most frequent lesions are osteoporosis of the metaphysial line, interphalangeal arthrosis, and hallux valgus. A total of 7 perforating plantar ulcers were noted, and the physiopathology was similar to that described in insulin-dependent diabetes. Lowered insulin levels and the neuropathy which this produres are the major etiological factors, but foot deformities such as talipes equinus and/or flat-feet play a determining role. Treatment consists of insulin administration and avoidance of plantar pressure at the site of the lesions.", "contents": "[Foot lesions in non-insulin-dependent diabetes. A report on 25 cases (author's transl)]. Diabetic lesions in 25 patients with maturity-onset non-insulin-dependent diabetes, but with neuropathy and even other complications in spite of previous treatment, were studied. Distribution according to the duration of the diabetes and the age of the patients was comparable to that seen in insulin-dependent diabetes. The most frequent lesions are osteoporosis of the metaphysial line, interphalangeal arthrosis, and hallux valgus. A total of 7 perforating plantar ulcers were noted, and the physiopathology was similar to that described in insulin-dependent diabetes. Lowered insulin levels and the neuropathy which this produres are the major etiological factors, but foot deformities such as talipes equinus and/or flat-feet play a determining role. Treatment consists of insulin administration and avoidance of plantar pressure at the site of the lesions."}
{"id": "PMID:475209", "title": "[Pulmonary mycobacteriosis due to Mycobacterium chelonei: a report on a new case (authors' transl)].", "content": "Mycobacterium chelonei is a saprophytic germ usually devoid of pathogenic activity. Over a period of about the last ten years, however, several cases have been reported, including twelve cases of bronchopulmonary affections, in which it has been the infecting organism. The radiographic appearance is in every respect similar to that observed in pulmonary tuberculosis. A positive diagnosis of infection due to this germ can be made by the absence of Kuch's bacillus the lack of therapeutic effect of antituberculous medication, a positive skin reaction to specific antigens, and positive Mycobacterium Chelonei cultures from biopsy specimens. A new case of this infection is reported.", "contents": "[Pulmonary mycobacteriosis due to Mycobacterium chelonei: a report on a new case (authors' transl)]. Mycobacterium chelonei is a saprophytic germ usually devoid of pathogenic activity. Over a period of about the last ten years, however, several cases have been reported, including twelve cases of bronchopulmonary affections, in which it has been the infecting organism. The radiographic appearance is in every respect similar to that observed in pulmonary tuberculosis. A positive diagnosis of infection due to this germ can be made by the absence of Kuch's bacillus the lack of therapeutic effect of antituberculous medication, a positive skin reaction to specific antigens, and positive Mycobacterium Chelonei cultures from biopsy specimens. A new case of this infection is reported."}
{"id": "PMID:475210", "title": "[Zollinger-Ellison syndrome with high blood calcium levels resembling the neoplastic type (author's transl)].", "content": "A patient with the Zollinger-Ellison syndrome was found to have increased blood gastrin levels associated with the secretion of a compound which produced high blood calcium levels. This compound differed from immunoreactive parathormone, and caused the clinical picture of primary neoplastic hyperparathyroidism. This case is compared with other cases of multiple-secreting Zollinger-Ellison syndrome reported in the published literature, within the framework of the \"A.P.U.D.\" system.", "contents": "[Zollinger-Ellison syndrome with high blood calcium levels resembling the neoplastic type (author's transl)]. A patient with the Zollinger-Ellison syndrome was found to have increased blood gastrin levels associated with the secretion of a compound which produced high blood calcium levels. This compound differed from immunoreactive parathormone, and caused the clinical picture of primary neoplastic hyperparathyroidism. This case is compared with other cases of multiple-secreting Zollinger-Ellison syndrome reported in the published literature, within the framework of the \"A.P.U.D.\" system."}
{"id": "PMID:475211", "title": "[Is the primary lesion in Behcet's disease a venous \"tropism\" vascularity? (author's transl)].", "content": "The authors studied eight cases of Behcet's disease, all of which were characterized by different degrees of phlebitis disorders. They also reviewed the anatomical and clinical features of a similar type, reported in the published literature. They stress the frequency and severity of changes in the veins in the localization of the various symptomatic lesions of the affection. From these results, they conclude that the primary lesion in this disease could be a phenomenon of vascularity of mainly venous-\"tropism\" which would explain the proteiform clinical features of Behcet's aphthosis.", "contents": "[Is the primary lesion in Behcet's disease a venous \"tropism\" vascularity? (author's transl)]. The authors studied eight cases of Behcet's disease, all of which were characterized by different degrees of phlebitis disorders. They also reviewed the anatomical and clinical features of a similar type, reported in the published literature. They stress the frequency and severity of changes in the veins in the localization of the various symptomatic lesions of the affection. From these results, they conclude that the primary lesion in this disease could be a phenomenon of vascularity of mainly venous-\"tropism\" which would explain the proteiform clinical features of Behcet's aphthosis."}
{"id": "PMID:475212", "title": "[A case of distal mesenteric arteritis (author's transl)].", "content": "A case is reported of distal mesenteric arteritis discovered during operation and confirmed by arteriography. The arteritis was probably not inflammatory in origin, but could have been due to atheroma. Only one other case of this type has been reported.", "contents": "[A case of distal mesenteric arteritis (author's transl)]. A case is reported of distal mesenteric arteritis discovered during operation and confirmed by arteriography. The arteritis was probably not inflammatory in origin, but could have been due to atheroma. Only one other case of this type has been reported."}
{"id": "PMID:475213", "title": "[Acute and chronic tuberculosis of the liver and hematopoietic system (author's transl)].", "content": "The authors report three cases of tuberculosis affecting the liver and hematopoietic system. The difficulty in diagnosing the chronic progressive forms of the disease and the severity of the acute forms is stressed. Liver biopsy is considered to be the most effective examination for confirming the nature of the disease. Acute and chronic forms can also be distinguished histologically, as the former produce nectotic lesions rich in tubercle bacillus but with only slight surrounding cellular reaction, while the latter lesions are productive and are rich in epithelioid histocytic cells.", "contents": "[Acute and chronic tuberculosis of the liver and hematopoietic system (author's transl)]. The authors report three cases of tuberculosis affecting the liver and hematopoietic system. The difficulty in diagnosing the chronic progressive forms of the disease and the severity of the acute forms is stressed. Liver biopsy is considered to be the most effective examination for confirming the nature of the disease. Acute and chronic forms can also be distinguished histologically, as the former produce nectotic lesions rich in tubercle bacillus but with only slight surrounding cellular reaction, while the latter lesions are productive and are rich in epithelioid histocytic cells."}
{"id": "PMID:475214", "title": "[Constrictive pericarditis complicating quiescent rheumatoid arthritis (author's transl)].", "content": "A brief report is given of an extremely rare case of pericardial constriction in a patient with quiescent sero-negative rheumatoid arthritis. Pericardectomy was only partially completed as it was impossible to divide the bands joining the peri- and epicardium.", "contents": "[Constrictive pericarditis complicating quiescent rheumatoid arthritis (author's transl)]. A brief report is given of an extremely rare case of pericardial constriction in a patient with quiescent sero-negative rheumatoid arthritis. Pericardectomy was only partially completed as it was impossible to divide the bands joining the peri- and epicardium."}
{"id": "PMID:475215", "title": "[Grawitz's tumor and provoked hyperlipemia (author's transl)].", "content": "After reporting a case of a patient aged 69 years with an inoperable hypernephroma, in whom the presence of a polycythemia and a reduction in the provoked hyperlipemia curve was found to co-exist, the authors review the data in the published literature on the influence on the small intestine of cancers other than those in the gastro-intestinal system. Basing their comments on studies of hypernephromas, they stress that a reduction or delay in the hyperlipemia curve was found in 23 out of 28 patients. This abnormality can only be explained by hypotheses at the present time.", "contents": "[Grawitz's tumor and provoked hyperlipemia (author's transl)]. After reporting a case of a patient aged 69 years with an inoperable hypernephroma, in whom the presence of a polycythemia and a reduction in the provoked hyperlipemia curve was found to co-exist, the authors review the data in the published literature on the influence on the small intestine of cancers other than those in the gastro-intestinal system. Basing their comments on studies of hypernephromas, they stress that a reduction or delay in the hyperlipemia curve was found in 23 out of 28 patients. This abnormality can only be explained by hypotheses at the present time."}
{"id": "PMID:475216", "title": "[\"In vitro\" interactions between influenza virus and mouse lung alveolar macrophages (author's transl)].", "content": "Interactions between influenza virus A/PR/8/34 (H0N1) and Balb/c mouse lung alveolar macrophages have been studied in vitro. One day after initiation of alveolar macrophage culture in 35 mm Falcon dishes, the virus suspension was allowed to adsorb to the cells for 1 h. Detachment of cells from the plastic substrate, morphological changes in adherent cells and decreased phagocytosis of heat-killed Candida albicans occured slowly as compared to control cultures. These facts appeared to be directly correlated to the concentration of viruses in the inoculum. Data yielded by virus titrations, electron microscopy and immunofluorescence suggest that mouse lung alveolar macrophages are able to take up a large amount of viral particles and inhibit their replication, allowing only an abortive viral cycle.", "contents": "[\"In vitro\" interactions between influenza virus and mouse lung alveolar macrophages (author's transl)]. Interactions between influenza virus A/PR/8/34 (H0N1) and Balb/c mouse lung alveolar macrophages have been studied in vitro. One day after initiation of alveolar macrophage culture in 35 mm Falcon dishes, the virus suspension was allowed to adsorb to the cells for 1 h. Detachment of cells from the plastic substrate, morphological changes in adherent cells and decreased phagocytosis of heat-killed Candida albicans occured slowly as compared to control cultures. These facts appeared to be directly correlated to the concentration of viruses in the inoculum. Data yielded by virus titrations, electron microscopy and immunofluorescence suggest that mouse lung alveolar macrophages are able to take up a large amount of viral particles and inhibit their replication, allowing only an abortive viral cycle."}
{"id": "PMID:475217", "title": "Perithecial ultrastructure and formation of ascospores of Ceratocystis stenoceras (Robak) C. Moreau.", "content": "Fine details of perithecial morphology and aspects of ascospore formation of Ceratocystis stenoceras (Robak) C. Moreau are shown in electron micrographs of ultrathin sections. The envelope of the perithecial body proper consisted of two zones of cells which differed morphologically one from another. Loose aggregates of small electron opaque particles were present at the outer wall surface which may be responsible for the characteristic pigmentation of the body and neck. Cells comprising the ostiolate neck may arise as modifications of spindle-shaped cells of the inner zone of the perithecial envelope. Cell walls of the neck and of zones 1 and 2 may be composed in part of periodic acid-reactive polysaccharide. The perithecial envelope and ascogenous cells were separated by a band of several parallel, double-layered membranes which may function in some manner with biosynthetic activities of ascospore production. In general, the mechanics of ascospore formation by C. stenoceras were in most ways in agreement with recent reports of ascospore outogeny in other ascomycetous fungi. Mature ascospores were somewhat lenticular in shape and the outer space wall was finely sculptured.", "contents": "Perithecial ultrastructure and formation of ascospores of Ceratocystis stenoceras (Robak) C. Moreau. Fine details of perithecial morphology and aspects of ascospore formation of Ceratocystis stenoceras (Robak) C. Moreau are shown in electron micrographs of ultrathin sections. The envelope of the perithecial body proper consisted of two zones of cells which differed morphologically one from another. Loose aggregates of small electron opaque particles were present at the outer wall surface which may be responsible for the characteristic pigmentation of the body and neck. Cells comprising the ostiolate neck may arise as modifications of spindle-shaped cells of the inner zone of the perithecial envelope. Cell walls of the neck and of zones 1 and 2 may be composed in part of periodic acid-reactive polysaccharide. The perithecial envelope and ascogenous cells were separated by a band of several parallel, double-layered membranes which may function in some manner with biosynthetic activities of ascospore production. In general, the mechanics of ascospore formation by C. stenoceras were in most ways in agreement with recent reports of ascospore outogeny in other ascomycetous fungi. Mature ascospores were somewhat lenticular in shape and the outer space wall was finely sculptured."}
{"id": "PMID:475218", "title": "Salmonella 38:g,z51:-, a new Salmonella of sub-genus IV: a serological study on the complex H-antigen g,z51 which can be divided into three sub-genus specific factors.", "content": "A new Salmonella of sub-genus IV with the antigen formula 38:g,z51:- has been isolated from a boa. Comparative studies of the H:g,z51 revealed that this phase only occurs in the sub-genera I, III and IV but not in sub-genus II. Furthermore it became evident that there exist typical serological differences between the g,z51 phases of Salmonella sub-genus I, III and IV which are of significance for the differential diagnosis.", "contents": "Salmonella 38:g,z51:-, a new Salmonella of sub-genus IV: a serological study on the complex H-antigen g,z51 which can be divided into three sub-genus specific factors. A new Salmonella of sub-genus IV with the antigen formula 38:g,z51:- has been isolated from a boa. Comparative studies of the H:g,z51 revealed that this phase only occurs in the sub-genera I, III and IV but not in sub-genus II. Furthermore it became evident that there exist typical serological differences between the g,z51 phases of Salmonella sub-genus I, III and IV which are of significance for the differential diagnosis."}
{"id": "PMID:475219", "title": "[First steps in the bacterial colonization of the digestive tract of neonates (author's transl)].", "content": "Meconium samples obtained from 14 children aged 18 to 51 h were subjected to a quantitative differential analysis. The meconium of one of the children (47 h old) was sterile; in those of the 11 infants aged less than 47 h the dominant flora was composed of either enterobacteria, mainly Escherichia coli sensitive to usual antibiotics, or of streptococci. The genus Acinetobacter constituted the dominant flora in one case only. Staphylococcus, Corynebacterium, Clostridium (C. perfringens), Bacteroides, Peptococcus, when present in the meconium, represented a very small proportion of the total flora. In the two children aged 48 h and more, the flora was more complex and Bacteroides, Bifidobacterium, Veillonella, Peptostreptococcus, Clostridium and Staphylococcus were associated to streptococci and enterobacteria. A very small number of lactobacilli was found in one child only. No yeasts were detected. The sequence of establishment of the various bacteria was studied in a child (OG) by an analysis of 8 samples obtained between 0 and 74 h after its birth. E. coli and Streptococcus became established 24 h after parturition, Bifidobacterium and Bacteroides the 2nd and 3rd day, respectively. Lactobacillus and Peptococcus were only present in very low number in the first 2 meconium samples and did not become established in the child, although they were present in a rather large number in the vaginal and faecal samples from the mother. In addition, use of axenic mice inoculated with the 3rd meconium from the infant OG revealed the existence of certain genera of bacteria probably of faecal origin which had not been detected by the direct analysis of the meconium. These genera did not become established in the child. The hypothesis according to which the meconium chooses among the fortuitous contaminations before true colonization of the digestive tract is established, is discussed. It was also observed that the evolution of the ratio between the number of E. coli and Proteus morganii in the successive samples obtained from OG was very different from that observed in the mice inoculated with these samples.", "contents": "[First steps in the bacterial colonization of the digestive tract of neonates (author's transl)]. Meconium samples obtained from 14 children aged 18 to 51 h were subjected to a quantitative differential analysis. The meconium of one of the children (47 h old) was sterile; in those of the 11 infants aged less than 47 h the dominant flora was composed of either enterobacteria, mainly Escherichia coli sensitive to usual antibiotics, or of streptococci. The genus Acinetobacter constituted the dominant flora in one case only. Staphylococcus, Corynebacterium, Clostridium (C. perfringens), Bacteroides, Peptococcus, when present in the meconium, represented a very small proportion of the total flora. In the two children aged 48 h and more, the flora was more complex and Bacteroides, Bifidobacterium, Veillonella, Peptostreptococcus, Clostridium and Staphylococcus were associated to streptococci and enterobacteria. A very small number of lactobacilli was found in one child only. No yeasts were detected. The sequence of establishment of the various bacteria was studied in a child (OG) by an analysis of 8 samples obtained between 0 and 74 h after its birth. E. coli and Streptococcus became established 24 h after parturition, Bifidobacterium and Bacteroides the 2nd and 3rd day, respectively. Lactobacillus and Peptococcus were only present in very low number in the first 2 meconium samples and did not become established in the child, although they were present in a rather large number in the vaginal and faecal samples from the mother. In addition, use of axenic mice inoculated with the 3rd meconium from the infant OG revealed the existence of certain genera of bacteria probably of faecal origin which had not been detected by the direct analysis of the meconium. These genera did not become established in the child. The hypothesis according to which the meconium chooses among the fortuitous contaminations before true colonization of the digestive tract is established, is discussed. It was also observed that the evolution of the ratio between the number of E. coli and Proteus morganii in the successive samples obtained from OG was very different from that observed in the mice inoculated with these samples."}
{"id": "PMID:475220", "title": "[Glycosylation and isoelectric properties of complete and defective rabies viruses (author's transl)].", "content": "From a structural point of view an essential distinction between complete and defective rabies viruses is difference in size. In addition, isoelectric properties differ. The complete virus has an isoelectric point approaching neutrality, whereas the defective virus focuses between pH 3-4.7. The isoelectric points of the glycoprotein from complete and defective viruses differ in a corresponding fashion. The Pasteur virus cultivated on BHK21C13 cells, contains glycoproteins, the glycopeptides of which have a structure containing the following five monosaccharides: sialic acid, D-galactose, (N-acetyl)D-glucosamine, D-mannose and L-fucose. The glycosylation of the glycoproteins is different, at least in so far as the relative sialic acid/glucosamine ratio is concerned.", "contents": "[Glycosylation and isoelectric properties of complete and defective rabies viruses (author's transl)]. From a structural point of view an essential distinction between complete and defective rabies viruses is difference in size. In addition, isoelectric properties differ. The complete virus has an isoelectric point approaching neutrality, whereas the defective virus focuses between pH 3-4.7. The isoelectric points of the glycoprotein from complete and defective viruses differ in a corresponding fashion. The Pasteur virus cultivated on BHK21C13 cells, contains glycoproteins, the glycopeptides of which have a structure containing the following five monosaccharides: sialic acid, D-galactose, (N-acetyl)D-glucosamine, D-mannose and L-fucose. The glycosylation of the glycoproteins is different, at least in so far as the relative sialic acid/glucosamine ratio is concerned."}
{"id": "PMID:475221", "title": "[Effects of starvation on incorporation of glycine in organs of Philosamia cynthia walkeri].", "content": "The influence of starvation on protein synthesis in organs from growing Philosamia larvae has been determined. In the fat body, a rapid and continuous decrease in synthesis begins at the onset of starvation. A decrease also occurs in the carcass, but of lesser extent. In the intestine and silkgland, the synthesis increases significantly during the first 12 hours and decreases thereafter. In all organs, the acid soluble glycine pools are drastically modified by starvation for 48 hours: this amino-acid accumulates in the silkglands, remains at a rather stable level in the intestine but decreases in the fat body and carcass. No adjustement of these pools is found at the level of haemolymph acid soluble glycine which increases during the 48 hours. These results are discussed in the context of regulatory mechanisms on metabolic level during feeding or food deprivation periods.", "contents": "[Effects of starvation on incorporation of glycine in organs of Philosamia cynthia walkeri]. The influence of starvation on protein synthesis in organs from growing Philosamia larvae has been determined. In the fat body, a rapid and continuous decrease in synthesis begins at the onset of starvation. A decrease also occurs in the carcass, but of lesser extent. In the intestine and silkgland, the synthesis increases significantly during the first 12 hours and decreases thereafter. In all organs, the acid soluble glycine pools are drastically modified by starvation for 48 hours: this amino-acid accumulates in the silkglands, remains at a rather stable level in the intestine but decreases in the fat body and carcass. No adjustement of these pools is found at the level of haemolymph acid soluble glycine which increases during the 48 hours. These results are discussed in the context of regulatory mechanisms on metabolic level during feeding or food deprivation periods."}
{"id": "PMID:475233", "title": "[An assessment of caustic oesophageal burns in the child (author's transl)].", "content": "68 patients who might have had a caustic oesophageal burn were observed in the E.N.T. department of Enfants Malades Hospital between 1973 and 1977. We describe the causes, the age, the character of the burns analysed after systematic oesophagoscopy, and treatment. The results are studied taking into account the depth of the burn, the nature of the toxic agent and the therapeutic possibilities.", "contents": "[An assessment of caustic oesophageal burns in the child (author's transl)]. 68 patients who might have had a caustic oesophageal burn were observed in the E.N.T. department of Enfants Malades Hospital between 1973 and 1977. We describe the causes, the age, the character of the burns analysed after systematic oesophagoscopy, and treatment. The results are studied taking into account the depth of the burn, the nature of the toxic agent and the therapeutic possibilities."}
{"id": "PMID:475234", "title": "[Nasopharyngeal fibroma (author's transl)].", "content": "The authors report 17 cases nasopharyngeal fibroma treated between 1966 and 1978. Half of the cases developed before the age of 16, the other half occurring between 16 and 28 years. Arteriography was performed in only 8 cases and embolisation in 4 cases with 2 vascular accidents. Surgery was performed in 16 cases (15 Rouge Denker, 1 paralatero nasal). Invasion of the pterygomaxillary fossa was invariably found at operation. Amongst the patients treated by surgery, there was only one local recurrence and reoperation was successful. Finally, one patient with a very large fibroma with invasion of the temporal fossa was successfully treated by radiotherapy at a dose of 4 000 R.", "contents": "[Nasopharyngeal fibroma (author's transl)]. The authors report 17 cases nasopharyngeal fibroma treated between 1966 and 1978. Half of the cases developed before the age of 16, the other half occurring between 16 and 28 years. Arteriography was performed in only 8 cases and embolisation in 4 cases with 2 vascular accidents. Surgery was performed in 16 cases (15 Rouge Denker, 1 paralatero nasal). Invasion of the pterygomaxillary fossa was invariably found at operation. Amongst the patients treated by surgery, there was only one local recurrence and reoperation was successful. Finally, one patient with a very large fibroma with invasion of the temporal fossa was successfully treated by radiotherapy at a dose of 4 000 R."}
{"id": "PMID:475235", "title": "[The use of a chondro-metallic prosthesis when the ossicular chain is destroyed (author's transl)].", "content": "The author describes a new prosthesis used in functional rehabilitation when the chain is destroyed. It consists of a tripod part in gold, anchored in the attic region with its foot resting on the plate of the stapes. This prosthesis is covered with a piece of autologous cartilage freshly removed from the tragus which forms the junction with the tympanic membrane or covering fascia. 60 cases are presented, some with a follow-up of 4 years. All of the patients were suffering from the sequelae of chronic otorrhoea, with varying degress of destruction of the chain and tympanic membrane, leaving a mobile plate. Functional rehabilitation in one stage was good in 66% of cases, poor in more than 8% of cases and nil in 25% of cases. Tolerance of the prosthesis was comparable to that of other metallic or plastic materials used in the middle ear. The plate of the stapes would appear to tolerate the prosthesis perfectly since no secondary labyrinthisation was seen. The author suggests the use of this chondro-metallic prosthesis was a reliable means of ensuring functional rehabilitation in the presence of a post-otitic empty middle ear.", "contents": "[The use of a chondro-metallic prosthesis when the ossicular chain is destroyed (author's transl)]. The author describes a new prosthesis used in functional rehabilitation when the chain is destroyed. It consists of a tripod part in gold, anchored in the attic region with its foot resting on the plate of the stapes. This prosthesis is covered with a piece of autologous cartilage freshly removed from the tragus which forms the junction with the tympanic membrane or covering fascia. 60 cases are presented, some with a follow-up of 4 years. All of the patients were suffering from the sequelae of chronic otorrhoea, with varying degress of destruction of the chain and tympanic membrane, leaving a mobile plate. Functional rehabilitation in one stage was good in 66% of cases, poor in more than 8% of cases and nil in 25% of cases. Tolerance of the prosthesis was comparable to that of other metallic or plastic materials used in the middle ear. The plate of the stapes would appear to tolerate the prosthesis perfectly since no secondary labyrinthisation was seen. The author suggests the use of this chondro-metallic prosthesis was a reliable means of ensuring functional rehabilitation in the presence of a post-otitic empty middle ear."}
{"id": "PMID:475236", "title": "[Audiovestibular exploration of the brain stem (author's transl)].", "content": "In the context of a postgraduate lecture for specialist physicians, the author describes techniques of objective audiology used on a daily basis in the exploration of the brain stem in the ENT department of Lariboisiere Hospital. The following are reviewed for each of them: --the corresponding anatomical pathways, --the principles and techniques used. The results are illustrated by examples. Increase in the threshold of stapes acoustic reflex above 95 dB on a frequency of 1,000 Hz is highly suggestive of acoustic nerve involvement or the lower part of the brain stem (bar sign). By contrast, the threshold is normal in endocochlear and supratentorial disorders. Comparison of abnormalities in stapes acoustic reflex in ipsi and contralateral stimulation, and in particular analysis of changes in their dynamics, often makes it possible to localise the problem to the acoustic nerve, the facial nerve or the brain stem itself. Recording of brain stem evoked auditory potentials (BERA), combined if necessary with that of the electro-cochleogram, is a delicate technique. This is the most useful and most precise audiological tool available to make a precise diagnosis of acoustic neuroma and to localise brain stem disease.", "contents": "[Audiovestibular exploration of the brain stem (author's transl)]. In the context of a postgraduate lecture for specialist physicians, the author describes techniques of objective audiology used on a daily basis in the exploration of the brain stem in the ENT department of Lariboisiere Hospital. The following are reviewed for each of them: --the corresponding anatomical pathways, --the principles and techniques used. The results are illustrated by examples. Increase in the threshold of stapes acoustic reflex above 95 dB on a frequency of 1,000 Hz is highly suggestive of acoustic nerve involvement or the lower part of the brain stem (bar sign). By contrast, the threshold is normal in endocochlear and supratentorial disorders. Comparison of abnormalities in stapes acoustic reflex in ipsi and contralateral stimulation, and in particular analysis of changes in their dynamics, often makes it possible to localise the problem to the acoustic nerve, the facial nerve or the brain stem itself. Recording of brain stem evoked auditory potentials (BERA), combined if necessary with that of the electro-cochleogram, is a delicate technique. This is the most useful and most precise audiological tool available to make a precise diagnosis of acoustic neuroma and to localise brain stem disease."}
{"id": "PMID:475237", "title": "[Three case histories of cortical and sub-cortical auditory lesions. Audiological and tomodensimetrical confrontations (author's transl)].", "content": "The authors describe three case histories of auditory lesions involving the cortex or subcortex of the temporal lobes. In two of the cases there are minor lesions of the hemisphere producing an amusia without verbal deafness or agnosia of non-verbal sounds. In one of the two patients, the total transitory deafness makes the use of the term cortical deafness debatable. In the third case, a bilateral vascular lesion of the temporal lobes had produced a pure verbal deafness without loss of tone discrimination or loss of the capacity to recognise changes in rhythm or sound frequency. In these three patients in whom there was no anatomical verification, the data given by the scanner and the study of evoked cortical potentials recorded over the two cerebral hemispheres allowed a fairly good study of the correlations between the topography of the lesions and their semiotic consequences.", "contents": "[Three case histories of cortical and sub-cortical auditory lesions. Audiological and tomodensimetrical confrontations (author's transl)]. The authors describe three case histories of auditory lesions involving the cortex or subcortex of the temporal lobes. In two of the cases there are minor lesions of the hemisphere producing an amusia without verbal deafness or agnosia of non-verbal sounds. In one of the two patients, the total transitory deafness makes the use of the term cortical deafness debatable. In the third case, a bilateral vascular lesion of the temporal lobes had produced a pure verbal deafness without loss of tone discrimination or loss of the capacity to recognise changes in rhythm or sound frequency. In these three patients in whom there was no anatomical verification, the data given by the scanner and the study of evoked cortical potentials recorded over the two cerebral hemispheres allowed a fairly good study of the correlations between the topography of the lesions and their semiotic consequences."}
{"id": "PMID:475239", "title": "[Petromastoid clearance using plastic surgery in the surveillance of large cavities. Re-suture of the ear canal (author's transl)].", "content": "The lesions met with in chronic otitis media and especially in cholesteatoma, can be extremely widespread and, after petromastoid clearance there is a large resulting cavity which is impossible to survey by the usual route of the ear canal. A plastic surgical technique on the ear canal, is described which allows an easy surveillance without esthetic damage. It consists of a resection of a part of the cartilage of the ear canal with conservation of the skin of the inner and outer ear canal. This skin is used to re-suture the piece of cartilage which has been sectioned.", "contents": "[Petromastoid clearance using plastic surgery in the surveillance of large cavities. Re-suture of the ear canal (author's transl)]. The lesions met with in chronic otitis media and especially in cholesteatoma, can be extremely widespread and, after petromastoid clearance there is a large resulting cavity which is impossible to survey by the usual route of the ear canal. A plastic surgical technique on the ear canal, is described which allows an easy surveillance without esthetic damage. It consists of a resection of a part of the cartilage of the ear canal with conservation of the skin of the inner and outer ear canal. This skin is used to re-suture the piece of cartilage which has been sectioned."}
{"id": "PMID:475240", "title": "Evaluation of computerized tomography, cinelaryngoscopy, and laryngography in determining the extent of laryngeal disease.", "content": "A prospective study of over 100 cases comparing computerized tomography (CT) and correlating these studies with photographic motion picture studies of the larynx, conventional tomography and contrast laryngography has been performed. The authors give illustrative examples of cases in which the CT scan has been documented as providing equal and often times greater information concerning not only tumors, but also cystic lesions and traumatic lesions. With the newer technology, the reduced radiation (which is less than one half that of conventional tomography), and the decreased expense (now comparable to that of laryngography alone), eliminates the need for conventional laryngography and tomography examinations. The incorporation of motion picture documentation of the lesions allowing future comparative studies between the original lesion and the CT are recommended for a more accurate retrospective classification and assessment of therapeutic results.", "contents": "Evaluation of computerized tomography, cinelaryngoscopy, and laryngography in determining the extent of laryngeal disease. A prospective study of over 100 cases comparing computerized tomography (CT) and correlating these studies with photographic motion picture studies of the larynx, conventional tomography and contrast laryngography has been performed. The authors give illustrative examples of cases in which the CT scan has been documented as providing equal and often times greater information concerning not only tumors, but also cystic lesions and traumatic lesions. With the newer technology, the reduced radiation (which is less than one half that of conventional tomography), and the decreased expense (now comparable to that of laryngography alone), eliminates the need for conventional laryngography and tomography examinations. The incorporation of motion picture documentation of the lesions allowing future comparative studies between the original lesion and the CT are recommended for a more accurate retrospective classification and assessment of therapeutic results."}
{"id": "PMID:475241", "title": "Leiomyosarcoma of the cervical trachea. Report of a case with reconstruction using a latissimus dorsi island flap.", "content": "A case of leiomyosarcoma of the cervical trachea is described, detailing clinical presentation, pathologic diagnosis and surgical management. The rarity of this tumor in this location accounted for difficulties in clinical diagnosis and pathologic classification. Surgical treatment involved resection of two thirds of the circumference of the infracricoid trachea, with partial resection of the cricoid cartilage and thyroid gland. The defect was reconstructed using bilateral \"butterfly\" advancement flaps and a latissimus dorsi myocutaneous island flap. The technique and advantages of the latissimus dorsi flap are briefly described.", "contents": "Leiomyosarcoma of the cervical trachea. Report of a case with reconstruction using a latissimus dorsi island flap. A case of leiomyosarcoma of the cervical trachea is described, detailing clinical presentation, pathologic diagnosis and surgical management. The rarity of this tumor in this location accounted for difficulties in clinical diagnosis and pathologic classification. Surgical treatment involved resection of two thirds of the circumference of the infracricoid trachea, with partial resection of the cricoid cartilage and thyroid gland. The defect was reconstructed using bilateral \"butterfly\" advancement flaps and a latissimus dorsi myocutaneous island flap. The technique and advantages of the latissimus dorsi flap are briefly described."}
{"id": "PMID:475242", "title": "Endoscopic Teflon keel for anterior glottic web.", "content": "Anterior glottic webs are generally repaired with a McNaught keel placed and removed by laryngofissure. Attempts have been made since 1922 to repair these webs endoscopically. An endoscopic technique using a Teflon keel which has been successful in properly selected cases is presented.", "contents": "Endoscopic Teflon keel for anterior glottic web. Anterior glottic webs are generally repaired with a McNaught keel placed and removed by laryngofissure. Attempts have been made since 1922 to repair these webs endoscopically. An endoscopic technique using a Teflon keel which has been successful in properly selected cases is presented."}
{"id": "PMID:475244", "title": "Benign tumors and lesions of the larynx in children. Surgical excision by CO2 laser.", "content": "Benign lesions of the airway in infants and children may endanger the airway and compromise laryngeal function. The CO2 laser provides an elegant method of transoral surgical excision without compromising laryngeal function or airway competence. It avoids the risks of such other treatment modalities as external surgical approaches, prolonged steroid therapy, and radiation. It is applicable to a wide variety of lesions without the necessity of tracheotomy. Hospitalization is minimized. Ten cases are presented to show the wide applicability of this technique. Gross pathology and pre- and postoperative findings are demonstrated and discussed, as is the safety and efficacy of the laser soft-tissue interaction.", "contents": "Benign tumors and lesions of the larynx in children. Surgical excision by CO2 laser. Benign lesions of the airway in infants and children may endanger the airway and compromise laryngeal function. The CO2 laser provides an elegant method of transoral surgical excision without compromising laryngeal function or airway competence. It avoids the risks of such other treatment modalities as external surgical approaches, prolonged steroid therapy, and radiation. It is applicable to a wide variety of lesions without the necessity of tracheotomy. Hospitalization is minimized. Ten cases are presented to show the wide applicability of this technique. Gross pathology and pre- and postoperative findings are demonstrated and discussed, as is the safety and efficacy of the laser soft-tissue interaction."}
{"id": "PMID:475246", "title": "Effects of irradiation of cervical lymph nodes.", "content": "The effects of irradiation upon the functional integrity of lymph nodes were studied in the isolated cervical region of the dog. After 6000 rads were applied in one dose plan, the measure of lymph flow was determined by the migration of radiogold from the larynx to the associated primary lymphatic terminals at intervals of 2, 4, and 12 weeks, and six months post-irradiation. Interference with lymph flow was identified by radionuclide stasis in the injection depot; alteration in the direction of flow was identified by relative concentration of the tracer item in the associated nodal terminals. The lymph flow was minimally distorted by irradiation in the test period unless secondary damage to the associated lymphatic channels intervened. The relative migration was consistent with the normal data in 52%, depressed in 13% and increased in 35% of the cases studied. Major obstruction and rerouting of lymph flow contralaterally was evident in 19% of the subjects due to fibrosis secondary to local infection.", "contents": "Effects of irradiation of cervical lymph nodes. The effects of irradiation upon the functional integrity of lymph nodes were studied in the isolated cervical region of the dog. After 6000 rads were applied in one dose plan, the measure of lymph flow was determined by the migration of radiogold from the larynx to the associated primary lymphatic terminals at intervals of 2, 4, and 12 weeks, and six months post-irradiation. Interference with lymph flow was identified by radionuclide stasis in the injection depot; alteration in the direction of flow was identified by relative concentration of the tracer item in the associated nodal terminals. The lymph flow was minimally distorted by irradiation in the test period unless secondary damage to the associated lymphatic channels intervened. The relative migration was consistent with the normal data in 52%, depressed in 13% and increased in 35% of the cases studied. Major obstruction and rerouting of lymph flow contralaterally was evident in 19% of the subjects due to fibrosis secondary to local infection."}
{"id": "PMID:475245", "title": "Clinical course of unusual malignant sarcomas of head and neck.", "content": "The clinical manifestations of 29 recently encountered sarcomas of the head and neck were analyzed in an attempt to define more accurately the diagnostic characteristics and therapeutic responses of these unusual tumors. The host factors of age, sex distribution, race, habits and associated features differ sufficiently to distinguish the sarcoma from the carcinoma population. In addition, the clinical course of sarcoma patients as monitored by mode of presentation, site of involvement, tumor histology, diagnostic features and certain elements of management and outcome, further indicates that these tumors can be defined and managed as a distinct group of lesions. A review of the recent literature supports the major conclusions of this study.", "contents": "Clinical course of unusual malignant sarcomas of head and neck. The clinical manifestations of 29 recently encountered sarcomas of the head and neck were analyzed in an attempt to define more accurately the diagnostic characteristics and therapeutic responses of these unusual tumors. The host factors of age, sex distribution, race, habits and associated features differ sufficiently to distinguish the sarcoma from the carcinoma population. In addition, the clinical course of sarcoma patients as monitored by mode of presentation, site of involvement, tumor histology, diagnostic features and certain elements of management and outcome, further indicates that these tumors can be defined and managed as a distinct group of lesions. A review of the recent literature supports the major conclusions of this study."}
{"id": "PMID:475247", "title": "Radiation fibrosis and necrosis of the larynx.", "content": "The deleterious effects of ionizing radiation upon the normal tissues of the larynx, even when it is not the specific target of the radiation therapist, are always apparent to the observant and experienced laryngologist. On occasions they are so marked as to obscure the diagnosis of concomitant persistent or recurrent cancer. There is a direct relationship between the number of rads delivered and the severity of the subsequent radiation reaction. A system of classifying such reactions is proposed. Grade 0 is normal. Grades I and II are expected. Grades III and IV may be considered complications and require specific and even energetic treatment. In grade IV reactions, which require operative intervention, laryngectomy may be the treatment of choice.", "contents": "Radiation fibrosis and necrosis of the larynx. The deleterious effects of ionizing radiation upon the normal tissues of the larynx, even when it is not the specific target of the radiation therapist, are always apparent to the observant and experienced laryngologist. On occasions they are so marked as to obscure the diagnosis of concomitant persistent or recurrent cancer. There is a direct relationship between the number of rads delivered and the severity of the subsequent radiation reaction. A system of classifying such reactions is proposed. Grade 0 is normal. Grades I and II are expected. Grades III and IV may be considered complications and require specific and even energetic treatment. In grade IV reactions, which require operative intervention, laryngectomy may be the treatment of choice."}
{"id": "PMID:475248", "title": "Cholesteatoma of the frontal and ethmoid areas.", "content": "Cholesteatomas of the frontoethmoid region must always be considered in the differential diagnosis of a mass in the frontoethmoid region. A painless, slow-growing mass, often accompanied by proptosis and diplopia, but without history of trauma or infection, should make one suspect  of this pathologic entity. Although histologically a benign lesion, the keratinizing squamous epithelial lining will continue to desquamate and expand, leading to erosion of surrounding structures which could be life-threatening. In order to prevent recurrence, complete removal of the cyst lining is mandatory. Cosmetic reconstruction, often requiring obliteration of the surgical cavity, should be delayed for one year to insure against recurrence of the covering of active squamous tissue adjacent to dura, cribriform plate or ocular structures.", "contents": "Cholesteatoma of the frontal and ethmoid areas. Cholesteatomas of the frontoethmoid region must always be considered in the differential diagnosis of a mass in the frontoethmoid region. A painless, slow-growing mass, often accompanied by proptosis and diplopia, but without history of trauma or infection, should make one suspect  of this pathologic entity. Although histologically a benign lesion, the keratinizing squamous epithelial lining will continue to desquamate and expand, leading to erosion of surrounding structures which could be life-threatening. In order to prevent recurrence, complete removal of the cyst lining is mandatory. Cosmetic reconstruction, often requiring obliteration of the surgical cavity, should be delayed for one year to insure against recurrence of the covering of active squamous tissue adjacent to dura, cribriform plate or ocular structures."}
{"id": "PMID:475250", "title": "Recurrent layngeal nerve section for spasmodic dysphonia.", "content": "Spasmodic dysphonia is a severe disorder of phonation accompanied by extreme tension of the entire phonatory system. The expressive functions of speech such as laughter, singing, and whispering are much less affected if at all. Psychotherapy, speech therapy, stimulant and psychotropic drugs, hypnotism and acupuncture have all been tried as treatment without success. In 1976, Dedo reported 34 patients who were managed with recurrent laryngeal nerve section for spasmodic dysphonia. All of these patients had marked improvement in voice with relief of spasticity. Twenty-two patients with documented spasmodic dysphonia present for at least one year have been managed at the Cleveland Clinic since Dedo's report. None of them had any improvement with conventional voice therapy and were subjected, therefore, to recurrent laryngeal nerve section.", "contents": "Recurrent layngeal nerve section for spasmodic dysphonia. Spasmodic dysphonia is a severe disorder of phonation accompanied by extreme tension of the entire phonatory system. The expressive functions of speech such as laughter, singing, and whispering are much less affected if at all. Psychotherapy, speech therapy, stimulant and psychotropic drugs, hypnotism and acupuncture have all been tried as treatment without success. In 1976, Dedo reported 34 patients who were managed with recurrent laryngeal nerve section for spasmodic dysphonia. All of these patients had marked improvement in voice with relief of spasticity. Twenty-two patients with documented spasmodic dysphonia present for at least one year have been managed at the Cleveland Clinic since Dedo's report. None of them had any improvement with conventional voice therapy and were subjected, therefore, to recurrent laryngeal nerve section."}
{"id": "PMID:475251", "title": "Laryngeal nerve crush for spastic dysphonia.", "content": "Four patients with spastic dysphonia refractory to speech and phychiatric therapy were treated by crushing the recurrent laryngeal nerve. Vocal cord paralysis was produced in all patients. Vocal spasticity subsided in all patients. Vocal cord motion returned in four to six months. Three of four patients remained free of spasticity for a minimum of 24 months.", "contents": "Laryngeal nerve crush for spastic dysphonia. Four patients with spastic dysphonia refractory to speech and phychiatric therapy were treated by crushing the recurrent laryngeal nerve. Vocal cord paralysis was produced in all patients. Vocal spasticity subsided in all patients. Vocal cord motion returned in four to six months. Three of four patients remained free of spasticity for a minimum of 24 months."}
{"id": "PMID:475249", "title": "The problem of the collapsing upper lateral cartilage.", "content": "The collapsing upper lateral cartilage is an airway problem that admits to no ready solution, such as the deviated nasal septum or deflected columella. It is my understanding from experienced nasal surgeons that an attack directly on the upper lateral cartilage has not provided a solution, but in general makes the problem worse. A look at the nasal septal base by simultaneous viewing of both nasal chambers may reveal a dimenionsal of 1 cm or more. In that event, a fairly radical narrowing of the septal base in the plane of the pyriform crest by a subperiosteal and subperichondrial approach with well-placed transfixion sutures can increase the air-flow across that plane. It has done so in eight patients. This operation is for the experienced nasal surgeon, or the otolaryngologist who has prepared himself by careful cadaver preexperience.", "contents": "The problem of the collapsing upper lateral cartilage. The collapsing upper lateral cartilage is an airway problem that admits to no ready solution, such as the deviated nasal septum or deflected columella. It is my understanding from experienced nasal surgeons that an attack directly on the upper lateral cartilage has not provided a solution, but in general makes the problem worse. A look at the nasal septal base by simultaneous viewing of both nasal chambers may reveal a dimenionsal of 1 cm or more. In that event, a fairly radical narrowing of the septal base in the plane of the pyriform crest by a subperiosteal and subperichondrial approach with well-placed transfixion sutures can increase the air-flow across that plane. It has done so in eight patients. This operation is for the experienced nasal surgeon, or the otolaryngologist who has prepared himself by careful cadaver preexperience."}
{"id": "PMID:475253", "title": "Intercellular junctions in the differentiating rat otocyst.", "content": "Intercellular junctions between cells of the rat otocyst on the 12th day of gestation were studied using lanthanum tracer and freeze-fracture techniques. At the luminal surface, the intercellular space is closed by a series of tight junctions. Gap junctions are also present between cells both within and below the luminal junctional complex. The presence of tight and gap junctions at this early stage in the differentiating otocyst is probably essential for the development of a normally functioning adult ear.", "contents": "Intercellular junctions in the differentiating rat otocyst. Intercellular junctions between cells of the rat otocyst on the 12th day of gestation were studied using lanthanum tracer and freeze-fracture techniques. At the luminal surface, the intercellular space is closed by a series of tight junctions. Gap junctions are also present between cells both within and below the luminal junctional complex. The presence of tight and gap junctions at this early stage in the differentiating otocyst is probably essential for the development of a normally functioning adult ear."}
{"id": "PMID:475252", "title": "Electrical stimulation of the auditory nerve in cats. Long term electrophysiological and histological results.", "content": "Each of five cats (one congenitally deaf) had Pt-Ir electrodes placed in the modiolus. Some electrodes were stimulated with a 100 micronamp, 0.25 msec balanced biphasic 50/sec pulse for 2 hours for a total of 20 to 40 hours each over periods of several months. Pre- and poststimulation measures of click-evoked N1 responses, averaged brain stem potentials, and impedances showed no long-term effects of damage to the nerves. Light microscopy showed very acceptable tissue tolerance and no evidence of damage caused by electrical stimulation. The deaf cat had about 10% residual nerve fibers which responded to stimulation.", "contents": "Electrical stimulation of the auditory nerve in cats. Long term electrophysiological and histological results. Each of five cats (one congenitally deaf) had Pt-Ir electrodes placed in the modiolus. Some electrodes were stimulated with a 100 micronamp, 0.25 msec balanced biphasic 50/sec pulse for 2 hours for a total of 20 to 40 hours each over periods of several months. Pre- and poststimulation measures of click-evoked N1 responses, averaged brain stem potentials, and impedances showed no long-term effects of damage to the nerves. Light microscopy showed very acceptable tissue tolerance and no evidence of damage caused by electrical stimulation. The deaf cat had about 10% residual nerve fibers which responded to stimulation."}
{"id": "PMID:475261", "title": "[Cutaneous symptoms of carcinoid tumours of the small bowel (author's transl)].", "content": "Cutaneous symptoms of carcinoid tumours are classified in 4 groups: --Acute type: flusch and vasomotor signs (the caracters of which are described). --Chronic type: telangiectasia, hyperpigmentation, sclerodermia-like, cutaneous maetastasis. --Rare type with non-diagnostic sign. --Types in association with other dermatological conditions either unrelated disease or with a likely related disease such as phacomatosis. Dermatological diagnosis is generally easy as cutaneous symptoms is concomitant with metastatic tumours and increased levels of serotonin and 5-H.I.A. The carcinoid syndrom may take place in the concept of the tumours of the A.P.U.D. system on clinical, embryological, histological and biochemical basis.", "contents": "[Cutaneous symptoms of carcinoid tumours of the small bowel (author's transl)]. Cutaneous symptoms of carcinoid tumours are classified in 4 groups: --Acute type: flusch and vasomotor signs (the caracters of which are described). --Chronic type: telangiectasia, hyperpigmentation, sclerodermia-like, cutaneous maetastasis. --Rare type with non-diagnostic sign. --Types in association with other dermatological conditions either unrelated disease or with a likely related disease such as phacomatosis. Dermatological diagnosis is generally easy as cutaneous symptoms is concomitant with metastatic tumours and increased levels of serotonin and 5-H.I.A. The carcinoid syndrom may take place in the concept of the tumours of the A.P.U.D. system on clinical, embryological, histological and biochemical basis."}
{"id": "PMID:475255", "title": "Brain stem potentials evoked by electrical stimulation of the cochlea in human subjects.", "content": "Brain stem potentials were recorded from scalp electrode to biphasic square wave electrical stimulation of implanted electrodes in the cochlea of three patients. Reliable potentials could be recorded that appeared 1.5 to 2.0 msec prior to the customary acoustically-evoked brain stem potentials. The effects of variations in electrical stimulus parameters of rate and intensity were measured. Brain stem potentials can provide objective indices of the effectiveness of electrical stimulation of the cochlea in man.", "contents": "Brain stem potentials evoked by electrical stimulation of the cochlea in human subjects. Brain stem potentials were recorded from scalp electrode to biphasic square wave electrical stimulation of implanted electrodes in the cochlea of three patients. Reliable potentials could be recorded that appeared 1.5 to 2.0 msec prior to the customary acoustically-evoked brain stem potentials. The effects of variations in electrical stimulus parameters of rate and intensity were measured. Brain stem potentials can provide objective indices of the effectiveness of electrical stimulation of the cochlea in man."}
{"id": "PMID:475254", "title": "Giant functioning cervicomediastinal parathyroid cyst.", "content": "A rare case of a large cervicomediastinal parathyroid cyst associated with hypercalcemia and an elevation of serum parathyroid hormone is presented. The pathogenesis of such a large cyst is discussed, hemorrhage into an adenoma being the most likely cause. Clinical investigations which should be carried out for any cystic mass at the root of the neck should include a serum calcium, and if this is found to be elevated, the possibility of the cyst being of parathyroid origin should be considered. In view of the high concentration of parathyroid hormone found within the cyst, aspiration of the cyst contents for parathyroid hormone estimation might well prove a valuable test to confirm the diagnosis.", "contents": "Giant functioning cervicomediastinal parathyroid cyst. A rare case of a large cervicomediastinal parathyroid cyst associated with hypercalcemia and an elevation of serum parathyroid hormone is presented. The pathogenesis of such a large cyst is discussed, hemorrhage into an adenoma being the most likely cause. Clinical investigations which should be carried out for any cystic mass at the root of the neck should include a serum calcium, and if this is found to be elevated, the possibility of the cyst being of parathyroid origin should be considered. In view of the high concentration of parathyroid hormone found within the cyst, aspiration of the cyst contents for parathyroid hormone estimation might well prove a valuable test to confirm the diagnosis."}
{"id": "PMID:475256", "title": "Micromethod for determination of middle ear gas composition.", "content": "This is a microchromatographic method for simultaneous determinations of O2, N2, CO2 and N2O in gas samples of 40-100 microliters. A Packard 836 U gas chromatograph with a thermal conductivity detector and helium gas as carrier was used. The combination of Porapak and 5A molecular sieve column system was found adequate and is described in detail. The fidelity of this method was proved by a high constancy of the retention time, the linearity of the response and the reproducibility of results. The present method proved to be reliable for determination of all middle ear gases in man and experimental animals during general anesthesia with N2O.", "contents": "Micromethod for determination of middle ear gas composition. This is a microchromatographic method for simultaneous determinations of O2, N2, CO2 and N2O in gas samples of 40-100 microliters. A Packard 836 U gas chromatograph with a thermal conductivity detector and helium gas as carrier was used. The combination of Porapak and 5A molecular sieve column system was found adequate and is described in detail. The fidelity of this method was proved by a high constancy of the retention time, the linearity of the response and the reproducibility of results. The present method proved to be reliable for determination of all middle ear gases in man and experimental animals during general anesthesia with N2O."}
{"id": "PMID:475257", "title": "Osteomas and exostoses of the external auditory canal. A clinical, histopathologic and scanning electron microscopic study.", "content": "Osteomas of the external auditory canal are considered clinically to be discrete, pedunculated bone lesions arising along the tympanosquamous suture. They are benign lesions but often are slowly progressive in size. Exostoses of the external auditory canal are broad-based elevations of bone usually multiple and bilaterally symmetric, involving the tympanic bone. It appears that both clinical and histopathologic evidence is sufficient to justify the impression that these two lesions should be considered separate entities and clinical diagnostic and histopathologic criteria have been suggested for diagnosis of these lesions.", "contents": "Osteomas and exostoses of the external auditory canal. A clinical, histopathologic and scanning electron microscopic study. Osteomas of the external auditory canal are considered clinically to be discrete, pedunculated bone lesions arising along the tympanosquamous suture. They are benign lesions but often are slowly progressive in size. Exostoses of the external auditory canal are broad-based elevations of bone usually multiple and bilaterally symmetric, involving the tympanic bone. It appears that both clinical and histopathologic evidence is sufficient to justify the impression that these two lesions should be considered separate entities and clinical diagnostic and histopathologic criteria have been suggested for diagnosis of these lesions."}
{"id": "PMID:475258", "title": "Accuracy of frozen section diagnosis of parotid lesions.", "content": "The accuracy of frozen section diagnosis was analyzed in a review of 132 parotid lesions. Of 107 benign lesions, 93% were correctly diagnosed on frozen section analysis, but of 25 malignant lesions, only 9 frozen sections were accurately interpreted. This study points out the difficulty encountered in using the frozen section technique when dealing with malignant parotid lesions and the importance of the surgeon's active participation in the analysis.", "contents": "Accuracy of frozen section diagnosis of parotid lesions. The accuracy of frozen section diagnosis was analyzed in a review of 132 parotid lesions. Of 107 benign lesions, 93% were correctly diagnosed on frozen section analysis, but of 25 malignant lesions, only 9 frozen sections were accurately interpreted. This study points out the difficulty encountered in using the frozen section technique when dealing with malignant parotid lesions and the importance of the surgeon's active participation in the analysis."}
{"id": "PMID:475268", "title": "Restenosis of the mitral valve.", "content": "It is suggested that restenosis is inevitable after mitral valvotomy and that only the time taken for it to occur is variable. The major factor affecting the lapse of time between operations is the extent of the original valvotomy. Calification of the valve is less important, and coincident mitral incompetence and the age of the patient have almost no influence on the time taken for restenosis to develop. A study has been made of 281 second closed valvotomies and 53 third ones. The operative mortality for second closed valvotomy was 6.7% and for third closed valvotomy 4%. Late emboli were rare. The average follow-up period after second valvotomies was 12.8 years: the condition of 29% of these patients was still satisfactory; 21% had had third valvotomies. At 5 years the condition of 53% was satisfactory. The average length of follow-up after third valvotomy was 6.4 years: the condition of 47% of patients was still satisfactory and the results were similar to those after second valvotomy. It is concluded that closed valvotomy remains the operation of choice when restenosis has occurred.", "contents": "Restenosis of the mitral valve. It is suggested that restenosis is inevitable after mitral valvotomy and that only the time taken for it to occur is variable. The major factor affecting the lapse of time between operations is the extent of the original valvotomy. Calification of the valve is less important, and coincident mitral incompetence and the age of the patient have almost no influence on the time taken for restenosis to develop. A study has been made of 281 second closed valvotomies and 53 third ones. The operative mortality for second closed valvotomy was 6.7% and for third closed valvotomy 4%. Late emboli were rare. The average follow-up period after second valvotomies was 12.8 years: the condition of 29% of these patients was still satisfactory; 21% had had third valvotomies. At 5 years the condition of 53% was satisfactory. The average length of follow-up after third valvotomy was 6.4 years: the condition of 47% of patients was still satisfactory and the results were similar to those after second valvotomy. It is concluded that closed valvotomy remains the operation of choice when restenosis has occurred."}
{"id": "PMID:475269", "title": "Urinary schistosomiasis in Saudi Arabia.", "content": "The distribution, aetiology, pathology, and clinical aspects of urinary schistosomiasis are outlined and it is emphasised that mortality from the disease is not due to the parasitic ova passed in the urine and faeces but to the complications caused by fibrotic immunological reactions to the retained ova. The treatment both of these complications and of the infestation itself is discussed. The special circumstances governing the transmission of the disease in Saudi Arabia are described and it is suggested that in view of these the energetic application of hygienic, educational, and therapeutic measures should make the complete eradication of schistosomiasis possible in that country.", "contents": "Urinary schistosomiasis in Saudi Arabia. The distribution, aetiology, pathology, and clinical aspects of urinary schistosomiasis are outlined and it is emphasised that mortality from the disease is not due to the parasitic ova passed in the urine and faeces but to the complications caused by fibrotic immunological reactions to the retained ova. The treatment both of these complications and of the infestation itself is discussed. The special circumstances governing the transmission of the disease in Saudi Arabia are described and it is suggested that in view of these the energetic application of hygienic, educational, and therapeutic measures should make the complete eradication of schistosomiasis possible in that country."}
{"id": "PMID:475270", "title": "The postural function of the iliotibial tract.", "content": "A new definition of the iliotibial tract is made. Its anatomical and physical characteristics are summarised and its known functions discussed. The various postures adopted by standing Man are looked at and one resting posture is closely analysed. Hence a new role is proposed for the iliotibial tract. In the hip bone a new bony effect of the iliotibial tract is proposed. The presence of this structure is traced in the fossil record and linked to the anthropological evidence of upright posture.", "contents": "The postural function of the iliotibial tract. A new definition of the iliotibial tract is made. Its anatomical and physical characteristics are summarised and its known functions discussed. The various postures adopted by standing Man are looked at and one resting posture is closely analysed. Hence a new role is proposed for the iliotibial tract. In the hip bone a new bony effect of the iliotibial tract is proposed. The presence of this structure is traced in the fossil record and linked to the anthropological evidence of upright posture."}
{"id": "PMID:475271", "title": "Duplication of the upper urinary tract.", "content": "The possibility of ureteric duplication should always be considered in children presenting with urinary infection. A high degree of suspicion should be present during X-ray investigation. Surgery is nearly always required to cure the problem, vesicoureteric reflux requiring reimplantation and a ureterocoele involving both uncapping and reimplantation techniques. Heminephroureterectomy is rarely required except in cases of bizarre ectopic opening in which incontinence is the presenting feature and the associated renal segment is dysplastic. Ureteropyelostomy is required for the rare situation of saddle reflux in the bifid ureter of incomplete duplication. It is occasionally employed for complete duplication, but it must be stressed that the primary defect is always at the lower end of the ureter and such bypass surgery must be combined with a ureterectomy and, when necessary, reimplantation of the remaining ureters.", "contents": "Duplication of the upper urinary tract. The possibility of ureteric duplication should always be considered in children presenting with urinary infection. A high degree of suspicion should be present during X-ray investigation. Surgery is nearly always required to cure the problem, vesicoureteric reflux requiring reimplantation and a ureterocoele involving both uncapping and reimplantation techniques. Heminephroureterectomy is rarely required except in cases of bizarre ectopic opening in which incontinence is the presenting feature and the associated renal segment is dysplastic. Ureteropyelostomy is required for the rare situation of saddle reflux in the bifid ureter of incomplete duplication. It is occasionally employed for complete duplication, but it must be stressed that the primary defect is always at the lower end of the ureter and such bypass surgery must be combined with a ureterectomy and, when necessary, reimplantation of the remaining ureters."}
{"id": "PMID:475272", "title": "The isolated circuit diathermy.", "content": "When using the conventional diathermy generator in surgery failure to apply the plate electrode can always present a serious risk of a thermoelectrical burn at any point where the patient makes contact with an earthed object on the operating table. The recent introduction of the earth-free (isolated circuit) diathermy should, under most conditions, provide complete protection against such risks. However, there are still certain circumstances under which even the isolated circuit may give rise to a thermoelectrical burn. Two such hypothetical examples are described; the sequences of events for these are fairly frequent occurrence.", "contents": "The isolated circuit diathermy. When using the conventional diathermy generator in surgery failure to apply the plate electrode can always present a serious risk of a thermoelectrical burn at any point where the patient makes contact with an earthed object on the operating table. The recent introduction of the earth-free (isolated circuit) diathermy should, under most conditions, provide complete protection against such risks. However, there are still certain circumstances under which even the isolated circuit may give rise to a thermoelectrical burn. Two such hypothetical examples are described; the sequences of events for these are fairly frequent occurrence."}
{"id": "PMID:475273", "title": "Comparison between general and local anaesthesia for repair of groin hernias.", "content": "A prospective study of 104 cases has been carried out to compare the use of local and general anaesthesia for the elective repair of groin hernias. There were no apparent disadvantages in using a local anaesthetic procedure. Patients having local anaesthetic herniorrhaphy required postoperative analgesics and antiemetics significantly less often and also returned home sooner (3.2 days) than patients having a general anaesthetic (4.1 days). These findings argue strongly in favour of local anaesthesia as a suitable alternative to general anaesthesia for repair of groin hernias.", "contents": "Comparison between general and local anaesthesia for repair of groin hernias. A prospective study of 104 cases has been carried out to compare the use of local and general anaesthesia for the elective repair of groin hernias. There were no apparent disadvantages in using a local anaesthetic procedure. Patients having local anaesthetic herniorrhaphy required postoperative analgesics and antiemetics significantly less often and also returned home sooner (3.2 days) than patients having a general anaesthetic (4.1 days). These findings argue strongly in favour of local anaesthesia as a suitable alternative to general anaesthesia for repair of groin hernias."}
{"id": "PMID:475274", "title": "Axillary hyperhidrosis: surgical cure with aesthetic scars.", "content": "By using a technique in which the hair-bearing skin of the axilla is undermined through three small incisions the eccrine glands can be methodically removed. The resulting scarring is minimal and is aesthetically acceptable. In a series of 25 patients (50 axillae) 23 were totally cured of their hyperhidrosis. The remaining 2 patients were sufficiently improved not to require any further treatment. The technique may be undertaken under either local or general anaesthesia.", "contents": "Axillary hyperhidrosis: surgical cure with aesthetic scars. By using a technique in which the hair-bearing skin of the axilla is undermined through three small incisions the eccrine glands can be methodically removed. The resulting scarring is minimal and is aesthetically acceptable. In a series of 25 patients (50 axillae) 23 were totally cured of their hyperhidrosis. The remaining 2 patients were sufficiently improved not to require any further treatment. The technique may be undertaken under either local or general anaesthesia."}
{"id": "PMID:475275", "title": "Kutler repair for the amputated fingertip.", "content": "Fingertip amputation is a common injury. Although the damage may not be extensive, the disability produced can be considerable. Different surgical procedures are available for reconstruction, but none is absolutely satisfactory. Twenty-four cases of fingertip amputation have been treated by primary skin closure using the Kutler bilateral V-Y advancement technique. The procedure was found to be simple and satisfactory, both functionally and cosmetically.", "contents": "Kutler repair for the amputated fingertip. Fingertip amputation is a common injury. Although the damage may not be extensive, the disability produced can be considerable. Different surgical procedures are available for reconstruction, but none is absolutely satisfactory. Twenty-four cases of fingertip amputation have been treated by primary skin closure using the Kutler bilateral V-Y advancement technique. The procedure was found to be simple and satisfactory, both functionally and cosmetically."}
{"id": "PMID:475276", "title": "PTFE (Goretex) femoropopliteal reconstruction for limb salvage.", "content": "We present the results of the use of polytetrafluoroethylene (PTFE, Goretex) grafts for limb salvage in 20 cases of femoropopliteal or more distal arterial reconstruction. There appears to be no relationship between graft survival and 'run-off' or the overall disease state of the vessels. Cumulative graft patency was almost 50% at 6 months, which is in contrast with higher patency rates reported from other countries.", "contents": "PTFE (Goretex) femoropopliteal reconstruction for limb salvage. We present the results of the use of polytetrafluoroethylene (PTFE, Goretex) grafts for limb salvage in 20 cases of femoropopliteal or more distal arterial reconstruction. There appears to be no relationship between graft survival and 'run-off' or the overall disease state of the vessels. Cumulative graft patency was almost 50% at 6 months, which is in contrast with higher patency rates reported from other countries."}
{"id": "PMID:475278", "title": "Management of the diabetic foot.", "content": "If the diabetic foot fails to respond adequately to a short period of basic primary treatment surgery must be considered. The main factor determining its nature and extent is the severity of large-vessel disease. If investigation shows this to be of minor significance local surgery is usually successful. In the presence of definite large-vessel disease, however, major amputation is usually necessary, though arterial reconstruction with local distal amputation may occasionally be feasible. The factors to be taken into consideration in the choice of treatment are discussed.", "contents": "Management of the diabetic foot. If the diabetic foot fails to respond adequately to a short period of basic primary treatment surgery must be considered. The main factor determining its nature and extent is the severity of large-vessel disease. If investigation shows this to be of minor significance local surgery is usually successful. In the presence of definite large-vessel disease, however, major amputation is usually necessary, though arterial reconstruction with local distal amputation may occasionally be feasible. The factors to be taken into consideration in the choice of treatment are discussed."}
{"id": "PMID:475299", "title": "Sudden postoperative death due to coronary thromboembolus.", "content": "In the last few years sudden deaths have been an object of active research in cardiology, as a high proportion of deaths from coronary arteriosclerosis are sudden and unexpected. In surgical materials, such sudden fatalities during the early postoperative period are relatively rare, because the patients are in the hospital under observation and intensive care can be started immediately in the event of coronary attacks. In the present rare case the patient died suddenly during the 5th postoperative night without any alarming symptoms of coronary thromboembolus.", "contents": "Sudden postoperative death due to coronary thromboembolus. In the last few years sudden deaths have been an object of active research in cardiology, as a high proportion of deaths from coronary arteriosclerosis are sudden and unexpected. In surgical materials, such sudden fatalities during the early postoperative period are relatively rare, because the patients are in the hospital under observation and intensive care can be started immediately in the event of coronary attacks. In the present rare case the patient died suddenly during the 5th postoperative night without any alarming symptoms of coronary thromboembolus."}
{"id": "PMID:475300", "title": "Pregnancy and small cell carcinoma of the uterine cervix.", "content": "A fatal case of a 22-year old pregnant patient with small cell cervical cancer is reported. The fulminant course of the disease was attributable more to the histological type of the tumour than to the pregnancy. Delay in diagnosis as a result of the pregnancy created a problem emphasized, by difficulty of cytological examination due to the very small size of the cancer cells.", "contents": "Pregnancy and small cell carcinoma of the uterine cervix. A fatal case of a 22-year old pregnant patient with small cell cervical cancer is reported. The fulminant course of the disease was attributable more to the histological type of the tumour than to the pregnancy. Delay in diagnosis as a result of the pregnancy created a problem emphasized, by difficulty of cytological examination due to the very small size of the cancer cells."}
{"id": "PMID:475301", "title": "Liver hilus dearterialization. A histological and electron microscopical study in the dog.", "content": "Liver histological and electron microscopical changes were studied in six dogs after liver hilus dearterialization and in two dogs after sham operation. All specimens were taken in vivo. In light microscopy a marked dilatation of the sinusoids was noted 90 minutes after dearterialization. After 24 hours it was even more pronounced. The hepatocytes were shrunken. On the seventh day these alterations persisted in the central area only. None of these changes were found in the sham-operated controls. In electron microscopy rounding and swelling of the mitochondria was observed on the first day after dearterialization. On the seventh day they looked normal. Damage of the endoplasmatic reticulum was found to persist even on the seventh day. The bile canaliculi membrane was damaged after dearterialization but not after sham operation. Dilatation of the sinusoids and widening of the space of Disse was observed on the first and seventh day after dearterialization. In the controls none of these changes were seen. In both dearterialized and sham-operated dogs a transient decrease in liver glycogen was noted. In conclusion, light and electron microscopical studies demonstrated reversible ischemic changes in the liver. These correlated well with our liver pO2 measurements which indicated transient hypoxia and our biochemical studies which indicated reversible damage of the liver.", "contents": "Liver hilus dearterialization. A histological and electron microscopical study in the dog. Liver histological and electron microscopical changes were studied in six dogs after liver hilus dearterialization and in two dogs after sham operation. All specimens were taken in vivo. In light microscopy a marked dilatation of the sinusoids was noted 90 minutes after dearterialization. After 24 hours it was even more pronounced. The hepatocytes were shrunken. On the seventh day these alterations persisted in the central area only. None of these changes were found in the sham-operated controls. In electron microscopy rounding and swelling of the mitochondria was observed on the first day after dearterialization. On the seventh day they looked normal. Damage of the endoplasmatic reticulum was found to persist even on the seventh day. The bile canaliculi membrane was damaged after dearterialization but not after sham operation. Dilatation of the sinusoids and widening of the space of Disse was observed on the first and seventh day after dearterialization. In the controls none of these changes were seen. In both dearterialized and sham-operated dogs a transient decrease in liver glycogen was noted. In conclusion, light and electron microscopical studies demonstrated reversible ischemic changes in the liver. These correlated well with our liver pO2 measurements which indicated transient hypoxia and our biochemical studies which indicated reversible damage of the liver."}
{"id": "PMID:475302", "title": "Liver damage after liver hilus dearterialization in dogs.", "content": "Twenty-one harrier dogs underwent a standardized liver hilus dearterialization with (7) or without (14) cholecystectomy using six sham-operated dogs as controls. Seven dogs died, only one of which was in the dearterialization and cholecystectomy group. S-OCT and s-GDH were increased at 90 minutes after dearterialization. There was no statistical difference between the groups at 90 minutes. On the first day after dearterialization, the activity of s-OCT, s-GDH, s-ALAT rose significantly (p less than 0.005). The activity of s-ASAT was, however, increased to the same extent both in the dearterialized and the sham-operated animals. A normalization of the serum enzyme activities was usually seen after one week. S-ALP increased on the first day (p less than 0.005) and still higher values were noted on the seventh day after dearterialization. Cholecystectomy did not affect any of these results except mortality. Liver oxygen consumption, in vitro was unchanged throughout the experiment. The oxygen consumption of bile duct mucosal scrapings one week after dearterialization was similar to the corresponding values in the controls. The results indicate that the dearterialization leads to a definite but mild and reversible mitochondrial and cell membrane damage. The results indicate further the need of adequate controls when the effects of dearterialization on the liver are studied.", "contents": "Liver damage after liver hilus dearterialization in dogs. Twenty-one harrier dogs underwent a standardized liver hilus dearterialization with (7) or without (14) cholecystectomy using six sham-operated dogs as controls. Seven dogs died, only one of which was in the dearterialization and cholecystectomy group. S-OCT and s-GDH were increased at 90 minutes after dearterialization. There was no statistical difference between the groups at 90 minutes. On the first day after dearterialization, the activity of s-OCT, s-GDH, s-ALAT rose significantly (p less than 0.005). The activity of s-ASAT was, however, increased to the same extent both in the dearterialized and the sham-operated animals. A normalization of the serum enzyme activities was usually seen after one week. S-ALP increased on the first day (p less than 0.005) and still higher values were noted on the seventh day after dearterialization. Cholecystectomy did not affect any of these results except mortality. Liver oxygen consumption, in vitro was unchanged throughout the experiment. The oxygen consumption of bile duct mucosal scrapings one week after dearterialization was similar to the corresponding values in the controls. The results indicate that the dearterialization leads to a definite but mild and reversible mitochondrial and cell membrane damage. The results indicate further the need of adequate controls when the effects of dearterialization on the liver are studied."}
{"id": "PMID:475303", "title": "Vascular access for haemodialysis with autogenous vein graft arteriovenous fistulas.", "content": "Nine autogenous vein grafts (AVG) have been used in nine patients to create arteriovenous fistulae for haemodialysis. All the patients lacked vessels for construction of a regular arteriovenous fistula. Three of the AVG failed during dialysis treatment 3, 10 and 14 months respectively following construction. Another AVG thrombosed but function was restored by thromboectomy. The same graft was later complicated by a false aneurysm and rupture but was then reconstructed with a vein patch. One AVG with a false aneurysm in the proximal anastomosis was repaired within six weeks.", "contents": "Vascular access for haemodialysis with autogenous vein graft arteriovenous fistulas. Nine autogenous vein grafts (AVG) have been used in nine patients to create arteriovenous fistulae for haemodialysis. All the patients lacked vessels for construction of a regular arteriovenous fistula. Three of the AVG failed during dialysis treatment 3, 10 and 14 months respectively following construction. Another AVG thrombosed but function was restored by thromboectomy. The same graft was later complicated by a false aneurysm and rupture but was then reconstructed with a vein patch. One AVG with a false aneurysm in the proximal anastomosis was repaired within six weeks."}
{"id": "PMID:475304", "title": "[Evolution of plasma concentration of gonadotropins and corticosterone during the growth of the male rat (author's transl)].", "content": "Plasma LH, FSH and corticosterone were determined between the 15th and 90th day of life, in normal male Rat. Plasma gonadotropins and corticosterone at basal conditions or after psychic aggression increased from the 15th to the 40th or 45th day of life; then all hormones decreased to adult age. The development of plasma gonadotropins and corticosterone are carried out in a parallel manner during the period studied. It was thought that perhaps the elevation of gonadotropins secretion might have stimulated the adrenals.", "contents": "[Evolution of plasma concentration of gonadotropins and corticosterone during the growth of the male rat (author's transl)]. Plasma LH, FSH and corticosterone were determined between the 15th and 90th day of life, in normal male Rat. Plasma gonadotropins and corticosterone at basal conditions or after psychic aggression increased from the 15th to the 40th or 45th day of life; then all hormones decreased to adult age. The development of plasma gonadotropins and corticosterone are carried out in a parallel manner during the period studied. It was thought that perhaps the elevation of gonadotropins secretion might have stimulated the adrenals."}
{"id": "PMID:475305", "title": "[Modifications of the accessory glands of male genital tract of intact and castrated rats after sulpiride treatment (author's transl)].", "content": "Sulpiride or Dogmatil (Delagrange, Paris) was injected to male rats at the rate of 40 mg/day for either 10 or 20 days. Ponderal fluctuations of the accessory organs followed by a quantitative analysis of fructose and of serum acid phosphatases issued from testis epididymis and prostates (PAP) were studied. The coagulating glands alone reacted strongly and their initial weight doubled in 20 days. On the contrary, the fructose decreased sharply in all accessory glands especially in the coagulating glands. The PAP decreased slightly. The fact the concentration of serum PAP remained relatively high even in the castrated males, can be explained by the increase in the lysosomial activity due to retention and diminution of secretion granules. Fructose and PAP are conditioned by androgens and their quantitative decrease suggests that the testosterone level decreased. Hyperprolactinemia induced by Sulpiride is concomittent to testosterone diminution and the effect of each hormone cannot easily be separated. In the castrated animals the effects of Sulpiride were minimized due to the difficulty in observing significant variations between treated and untreated castrates.", "contents": "[Modifications of the accessory glands of male genital tract of intact and castrated rats after sulpiride treatment (author's transl)]. Sulpiride or Dogmatil (Delagrange, Paris) was injected to male rats at the rate of 40 mg/day for either 10 or 20 days. Ponderal fluctuations of the accessory organs followed by a quantitative analysis of fructose and of serum acid phosphatases issued from testis epididymis and prostates (PAP) were studied. The coagulating glands alone reacted strongly and their initial weight doubled in 20 days. On the contrary, the fructose decreased sharply in all accessory glands especially in the coagulating glands. The PAP decreased slightly. The fact the concentration of serum PAP remained relatively high even in the castrated males, can be explained by the increase in the lysosomial activity due to retention and diminution of secretion granules. Fructose and PAP are conditioned by androgens and their quantitative decrease suggests that the testosterone level decreased. Hyperprolactinemia induced by Sulpiride is concomittent to testosterone diminution and the effect of each hormone cannot easily be separated. In the castrated animals the effects of Sulpiride were minimized due to the difficulty in observing significant variations between treated and untreated castrates."}
{"id": "PMID:475306", "title": "[Thyroid incorporation of radioactive iodine in neotenic tritons (Triturus helveticus Raz.). In vivo studies by high resolution radioautography. Comparison of iodine incorporation in adults and larvae].", "content": "In vivo kinetics of radioiodine incorporation have been established for 70 newts, collected from Triturus helveticus Raz. populations where spontaneous neoteny occurs. Fifty of these newts which appeared to present four degrees of neoteny (more or less metamorphosed), have been studied. Main results obtained were: 1) The intensity of the iodine incorporation varies according to the degree of neoteny. The higher is this one, the lower is the intensity. 2) In general, iodine uptake rates are more or less similar to these of adults, but more often the evolution of the 125I uptake approaches a plateau-curve. From thyroid radioautographies on some animals (17 neotenic and 2 adult newts) for which in vivo iodine kinetics have been examined and on 9 normal larvae in premetamorphosis or in metamorphosis, we have noticed in those neotenic thyroids, a very small number of silver grains on apical vesicles and colloidal lumina when the degree of neoteny is the highest. This number of marked apical vesicles and colloid is more important in the neotenics which have lost some larval characters. Silver grains on colloid droplets indicating thyroid hormone excretion are inexistent in the most larval neotenics, more numerous in most metamorphosed neotenics. But they always are less numerous than in metamorphosing larvae. For a few animals, the fixation curve is very high, probably because of a stimulation of thyroid function, induced by the test and not because of a goitrous tendancy, as radioautographic observation of their thyroid shows. Thyroid function and its relation with the origin of this total and accidental neoteny are discussed.", "contents": "[Thyroid incorporation of radioactive iodine in neotenic tritons (Triturus helveticus Raz.). In vivo studies by high resolution radioautography. Comparison of iodine incorporation in adults and larvae]. In vivo kinetics of radioiodine incorporation have been established for 70 newts, collected from Triturus helveticus Raz. populations where spontaneous neoteny occurs. Fifty of these newts which appeared to present four degrees of neoteny (more or less metamorphosed), have been studied. Main results obtained were: 1) The intensity of the iodine incorporation varies according to the degree of neoteny. The higher is this one, the lower is the intensity. 2) In general, iodine uptake rates are more or less similar to these of adults, but more often the evolution of the 125I uptake approaches a plateau-curve. From thyroid radioautographies on some animals (17 neotenic and 2 adult newts) for which in vivo iodine kinetics have been examined and on 9 normal larvae in premetamorphosis or in metamorphosis, we have noticed in those neotenic thyroids, a very small number of silver grains on apical vesicles and colloidal lumina when the degree of neoteny is the highest. This number of marked apical vesicles and colloid is more important in the neotenics which have lost some larval characters. Silver grains on colloid droplets indicating thyroid hormone excretion are inexistent in the most larval neotenics, more numerous in most metamorphosed neotenics. But they always are less numerous than in metamorphosing larvae. For a few animals, the fixation curve is very high, probably because of a stimulation of thyroid function, induced by the test and not because of a goitrous tendancy, as radioautographic observation of their thyroid shows. Thyroid function and its relation with the origin of this total and accidental neoteny are discussed."}
{"id": "PMID:475307", "title": "[Renal osteodystrophy in two children : a comparison of the effects of 1 alpha-hydroxycholecalciferol (author's transl)].", "content": "Two children suffering from renal osteodystrophy were treated by 1 alpha hydroxycholecalciferol (1 alpha OHD3) 1 microgr. each day, for 18 months. In both the level alkaline phosphatase decreased at the same time as endogenous immunoreactive CT increased, iPTH did not change steadily, whereas plasma creatinine rise. As plasma calcium concentration did not increase, it is suggested that the increase in endogenous CT concentration is a part of the favourable response to the treatment by 1 alpha OHD3.", "contents": "[Renal osteodystrophy in two children : a comparison of the effects of 1 alpha-hydroxycholecalciferol (author's transl)]. Two children suffering from renal osteodystrophy were treated by 1 alpha hydroxycholecalciferol (1 alpha OHD3) 1 microgr. each day, for 18 months. In both the level alkaline phosphatase decreased at the same time as endogenous immunoreactive CT increased, iPTH did not change steadily, whereas plasma creatinine rise. As plasma calcium concentration did not increase, it is suggested that the increase in endogenous CT concentration is a part of the favourable response to the treatment by 1 alpha OHD3."}
{"id": "PMID:475309", "title": "[Metabolic investigation of a population of institutionalized epileptics (author's transl)].", "content": "77 epileptics receiving a combination of anticonvulsants have been studied. 25(OH)D3 levels were diminished in 38% of the patients, markedly so in 25%. iPTH levels were elevated in 13%. Total, as well as ionized, serum calcium levels were significantly lower in the epileptic patients, as compared with a control population. Serum P, Mg, and plasma total CO2 levels remained unaltered. Alkaline phosphatase levels were increased, as well as gamma-GT levels. A transiliac bone biopsy was performed in 15 patients and histomorphometric studies were achieved on decalcified and undecalcified sections. Osteomalacia was present in 4 out of the 15 cases, hyperosteoidosis in one. Three biological features distinguished the cases with osteomalacia : iPTH levels and alkaline phosphatase values were significantly higher, and serum P levels were significantly lower (all were below 2,4 mg/dl) as compared with the non-osteomalacic patients. In an epileptic population, the serum Ca value is lower than in a control population by 0,5 mg/dl. When in addition the serum P is low (which was a feature of male patients), the danger exists for osteomalacia to develop. The former abnormality is connected with low 25(OH)D3 levels, the primary event, while the latter is probably related to high (secondary) iPTH levels.", "contents": "[Metabolic investigation of a population of institutionalized epileptics (author's transl)]. 77 epileptics receiving a combination of anticonvulsants have been studied. 25(OH)D3 levels were diminished in 38% of the patients, markedly so in 25%. iPTH levels were elevated in 13%. Total, as well as ionized, serum calcium levels were significantly lower in the epileptic patients, as compared with a control population. Serum P, Mg, and plasma total CO2 levels remained unaltered. Alkaline phosphatase levels were increased, as well as gamma-GT levels. A transiliac bone biopsy was performed in 15 patients and histomorphometric studies were achieved on decalcified and undecalcified sections. Osteomalacia was present in 4 out of the 15 cases, hyperosteoidosis in one. Three biological features distinguished the cases with osteomalacia : iPTH levels and alkaline phosphatase values were significantly higher, and serum P levels were significantly lower (all were below 2,4 mg/dl) as compared with the non-osteomalacic patients. In an epileptic population, the serum Ca value is lower than in a control population by 0,5 mg/dl. When in addition the serum P is low (which was a feature of male patients), the danger exists for osteomalacia to develop. The former abnormality is connected with low 25(OH)D3 levels, the primary event, while the latter is probably related to high (secondary) iPTH levels."}
{"id": "PMID:475310", "title": "[Osteomalacia in hyperphosphoethanolaminuria without hypophosphatasia (author's transl)].", "content": "Increased urinary excretion of phosphorylethanolamine (P.E.A.) is one of the salient features of hypophosphatasia. This inherited disorder is generally transmitted as an autosomial recessive trait and is characterized by abnormal mineralization of bone, premature loss of deciduous teeth and reduced tissue and serum alkaline phosphatases (A.P.) levels. The authors report a series of patients presenting with pains of skeletal origin attributed to an osteomalacia syndrome on the ground of a bone biopsy. These patients had no history of rickets during childhood but complained of early severe caries of the permanent dentition before the age of twenty. They had neither malabsorption nor renal tubular abnormalities. Their serum 25 OH vitamin D was normal and their serum A.P. levels were within the normal range with a normal isoenzyme distribution. All these patients had increased excretions of urinary P.E.A. and the latter correlate significantly with the degree of osteomalacia. Control patients with a malabsorption syndrome, showing osteomalacia and serum A.P. of the same degree of magnitude as the patients of the first group, have a normal P.E.A. excretion and no correlation appears between the degree of osteomalacia and the P.E.A. excretion. The cases with increased P.E.A. excretion may correspond to adult pseudohypophosphatasia. The signification of increased P.E.A. excretion is discussed.", "contents": "[Osteomalacia in hyperphosphoethanolaminuria without hypophosphatasia (author's transl)]. Increased urinary excretion of phosphorylethanolamine (P.E.A.) is one of the salient features of hypophosphatasia. This inherited disorder is generally transmitted as an autosomial recessive trait and is characterized by abnormal mineralization of bone, premature loss of deciduous teeth and reduced tissue and serum alkaline phosphatases (A.P.) levels. The authors report a series of patients presenting with pains of skeletal origin attributed to an osteomalacia syndrome on the ground of a bone biopsy. These patients had no history of rickets during childhood but complained of early severe caries of the permanent dentition before the age of twenty. They had neither malabsorption nor renal tubular abnormalities. Their serum 25 OH vitamin D was normal and their serum A.P. levels were within the normal range with a normal isoenzyme distribution. All these patients had increased excretions of urinary P.E.A. and the latter correlate significantly with the degree of osteomalacia. Control patients with a malabsorption syndrome, showing osteomalacia and serum A.P. of the same degree of magnitude as the patients of the first group, have a normal P.E.A. excretion and no correlation appears between the degree of osteomalacia and the P.E.A. excretion. The cases with increased P.E.A. excretion may correspond to adult pseudohypophosphatasia. The signification of increased P.E.A. excretion is discussed."}
{"id": "PMID:475311", "title": "[Erythrocyte metabolism in vitamin D deficiency rickets].", "content": "In an attempt to explain the hemolysis observed in vitamin D deficiency rickets in the infant, we studied erythrocytic glycolysis, the level of ATP and some of the key enzymes of the glycolytic pathway. This study was performed in one group of vitamin D deficient infants and in one control group. The values obtained in these two groups did not differ significantly. In the absence of a defect in the glycolytic pathway, we suggest that an anomaly exists in the red cell membrane of these patients.", "contents": "[Erythrocyte metabolism in vitamin D deficiency rickets]. In an attempt to explain the hemolysis observed in vitamin D deficiency rickets in the infant, we studied erythrocytic glycolysis, the level of ATP and some of the key enzymes of the glycolytic pathway. This study was performed in one group of vitamin D deficient infants and in one control group. The values obtained in these two groups did not differ significantly. In the absence of a defect in the glycolytic pathway, we suggest that an anomaly exists in the red cell membrane of these patients."}
{"id": "PMID:475312", "title": "[Metabolism of bone in culture (author's transl)].", "content": "A method for studying in vitro bone matrix resorption by the use of 35S injection was investigated. Various hormones were tested and their effects on 35S and 45Ca metabolism were compared.", "contents": "[Metabolism of bone in culture (author's transl)]. A method for studying in vitro bone matrix resorption by the use of 35S injection was investigated. Various hormones were tested and their effects on 35S and 45Ca metabolism were compared."}
{"id": "PMID:475313", "title": "[Effects of 1,25(OH)2D3 on bone mineralization in vitamin D deficiency (author's transl)].", "content": "Two cases with vitamin D-deficient osteomalacia of digestive origin (coeliac disease) have been treated orally with 1,25(OH)2D3 in dosages of respectively 8 and 3 microgram/24 h. Serial transiliac bone biopsies have been performed. Mineralization fronts were normalized after respectively 4 and 1.5 months, whereas mineralization rates, as demonstrated by double tetracycline labeling, were normalized respectively after 2 and 1.5 months. In both cases osteomalacia healed completely despite persistently low 25(OH)D levels in the serum. It is obvious from these studies that 1,25(OH)2D3 per se is able to cure vitamin D-deficient osteomalacia, a finding that has been disputed. Whether 25(OH)D acts more rapidly on bone, as suggested by the studies performed in one case with anticonvulsant osteomalacia, remains to be proven.", "contents": "[Effects of 1,25(OH)2D3 on bone mineralization in vitamin D deficiency (author's transl)]. Two cases with vitamin D-deficient osteomalacia of digestive origin (coeliac disease) have been treated orally with 1,25(OH)2D3 in dosages of respectively 8 and 3 microgram/24 h. Serial transiliac bone biopsies have been performed. Mineralization fronts were normalized after respectively 4 and 1.5 months, whereas mineralization rates, as demonstrated by double tetracycline labeling, were normalized respectively after 2 and 1.5 months. In both cases osteomalacia healed completely despite persistently low 25(OH)D levels in the serum. It is obvious from these studies that 1,25(OH)2D3 per se is able to cure vitamin D-deficient osteomalacia, a finding that has been disputed. Whether 25(OH)D acts more rapidly on bone, as suggested by the studies performed in one case with anticonvulsant osteomalacia, remains to be proven."}
{"id": "PMID:475314", "title": "[Bone histology and 25-OH vitamin D plasma levels in alcoholics without cirrhosis (author's transl)].", "content": "Bone mineralization is impaired in alcoholic non cirrhotics admitted to hospital for withdrawal. The defect is more marked in women than in men. Low 25-OH vitamin D plasma values may be observed in these patients and seem to be related to malnutrition. The degree of bone demineralization is not statistically correlated to 25-OH vitamin D plasma level.", "contents": "[Bone histology and 25-OH vitamin D plasma levels in alcoholics without cirrhosis (author's transl)]. Bone mineralization is impaired in alcoholic non cirrhotics admitted to hospital for withdrawal. The defect is more marked in women than in men. Low 25-OH vitamin D plasma values may be observed in these patients and seem to be related to malnutrition. The degree of bone demineralization is not statistically correlated to 25-OH vitamin D plasma level."}
{"id": "PMID:475315", "title": "[The effects of 1 alphahydroxycholecalciferol in patients with renal osteodystrophy, Paget's disease of bone and in normal subjects (author's transl)].", "content": "The administration of 1 alpha hydroxycholecalciferol (1 alpha OHD3) (2 microgr. per day) increase the plasma immunoreactive calcitonin (i CT) concentration in normal subjects after six days. This effect is also observed in patients suffering from Paget's disease of bone, suggesting that the mechanism responsable for the secretion of CT following the administration of 1 alpha is not disturbed in that disease. By contrast, the absence of increase in plasma iCT in the patients suffering from chronic renal failure suggests an impaired secretion of CT in that disorder.", "contents": "[The effects of 1 alphahydroxycholecalciferol in patients with renal osteodystrophy, Paget's disease of bone and in normal subjects (author's transl)]. The administration of 1 alpha hydroxycholecalciferol (1 alpha OHD3) (2 microgr. per day) increase the plasma immunoreactive calcitonin (i CT) concentration in normal subjects after six days. This effect is also observed in patients suffering from Paget's disease of bone, suggesting that the mechanism responsable for the secretion of CT following the administration of 1 alpha is not disturbed in that disease. By contrast, the absence of increase in plasma iCT in the patients suffering from chronic renal failure suggests an impaired secretion of CT in that disorder."}
{"id": "PMID:475316", "title": "[Radioimmunoassay of ADH in human urine (author's transl)].", "content": "A simple efficient procedure for extracting and concentrating arginine-8-vasopressin (AVP) from urine has been coupled with a specific and sensitive radioimmunoassay in order to measure antidiuretic hormone (ADH) excretion in normal humans under various physiological stimuli. Antisera have been raised in rabbits injected with lysine-vasopressin (LVP) or AVP coupled with bovine serum albumin. The antiserum selected for the assay which inhibits the antidiuresis induced in the rat by AVP is used at a final dilution of 1 : 50,000 and possesses a high association constant of 1 x 10(11) 1.mol-1. The limit of detection of the RIA system is 0.5 micronUI/ml of urine (1.25 pg). Urinary ADH has been extracted from urine by Miller and Moses method. Mean recovery of added vasopressin averaged 90.2% +/- 11 (SD) and assay of serial dilutions of such extracts showed that they behave in the assay system in the same way as synthetic AVP standards. Moreover comparison of the results obtained by the RIA to those given by the biological method using the ethanol anesthetized rat showed excellent correlation (r = 0.9 p less than 0.001). Under ad libitum fluid and food intake, mean daily urinary excretion of AVP (uncorrected for recovery) determined in 22 subjects was found to be 30.58 +/- 11.64 mU/h with no significant difference between men and women. In response to an oral waterload ADH became undetectable at the peak of diuresis. Following a 16 hr fluid deprivation, ADH rose moderately. A significant correlation has been found between urine osmolality and AVP excretion rate.", "contents": "[Radioimmunoassay of ADH in human urine (author's transl)]. A simple efficient procedure for extracting and concentrating arginine-8-vasopressin (AVP) from urine has been coupled with a specific and sensitive radioimmunoassay in order to measure antidiuretic hormone (ADH) excretion in normal humans under various physiological stimuli. Antisera have been raised in rabbits injected with lysine-vasopressin (LVP) or AVP coupled with bovine serum albumin. The antiserum selected for the assay which inhibits the antidiuresis induced in the rat by AVP is used at a final dilution of 1 : 50,000 and possesses a high association constant of 1 x 10(11) 1.mol-1. The limit of detection of the RIA system is 0.5 micronUI/ml of urine (1.25 pg). Urinary ADH has been extracted from urine by Miller and Moses method. Mean recovery of added vasopressin averaged 90.2% +/- 11 (SD) and assay of serial dilutions of such extracts showed that they behave in the assay system in the same way as synthetic AVP standards. Moreover comparison of the results obtained by the RIA to those given by the biological method using the ethanol anesthetized rat showed excellent correlation (r = 0.9 p less than 0.001). Under ad libitum fluid and food intake, mean daily urinary excretion of AVP (uncorrected for recovery) determined in 22 subjects was found to be 30.58 +/- 11.64 mU/h with no significant difference between men and women. In response to an oral waterload ADH became undetectable at the peak of diuresis. Following a 16 hr fluid deprivation, ADH rose moderately. A significant correlation has been found between urine osmolality and AVP excretion rate."}
{"id": "PMID:475317", "title": "[Experimental hepatic encephalopathy. The effect of portocaval shunt on the evolution of the anterior hypothalamic neurosecretion in the rat (author's transl)].", "content": "The evolution of the anterior hypothalamic neurosecretion is studied in control, sham-operated and portocaval shunt rats. In sham-operated related to control rats, no modification of the anterior hypothalamic neurosecretion is observed. In portocaval shunt rats, the hypothalamic neurosecretion increases. This increase is mainly observed into the median eminence in all the portocaval shunt rats. The hypothesis according to which the modification of neurosecretory acitivity of the hypothalamic structures may be related to a discrepancy between the central monoamines is discussed.", "contents": "[Experimental hepatic encephalopathy. The effect of portocaval shunt on the evolution of the anterior hypothalamic neurosecretion in the rat (author's transl)]. The evolution of the anterior hypothalamic neurosecretion is studied in control, sham-operated and portocaval shunt rats. In sham-operated related to control rats, no modification of the anterior hypothalamic neurosecretion is observed. In portocaval shunt rats, the hypothalamic neurosecretion increases. This increase is mainly observed into the median eminence in all the portocaval shunt rats. The hypothesis according to which the modification of neurosecretory acitivity of the hypothalamic structures may be related to a discrepancy between the central monoamines is discussed."}
{"id": "PMID:475318", "title": "[Digestive hormones and gastric diseases. Facts and hypotheses (author's transl)].", "content": "Relationships between hormonal secretions from the GI tract and gastric functional and/or pathological abnormalities could be studied according to 2 main lines : 1) gastric secretory changes could be the main symptom of hormonal secretory tumors, i.e. acid hypersecretion in the Zollinger Ellison syndrome, acid hyposecretion in pancreatic cholera and in somatostatinoma. In these cases, hormonal hypersecretion is directly responsible for the functional disturbances and the related symptoms; 2) gastric pathological conditions are sometimes accompanied by changes in hormonal secretion, but the level of interdependence is variable : high blood gastrin is directly depending upon the atrophic gastritis in pernicious anemia; this mechanism was also suggested in case of gastric carcinoma. Concerning ulcer disease, numerous problems are unsolved in respect to blood gastrin (basal and stimulated) abnormalities, as well as somatostatin and GIP secretions.", "contents": "[Digestive hormones and gastric diseases. Facts and hypotheses (author's transl)]. Relationships between hormonal secretions from the GI tract and gastric functional and/or pathological abnormalities could be studied according to 2 main lines : 1) gastric secretory changes could be the main symptom of hormonal secretory tumors, i.e. acid hypersecretion in the Zollinger Ellison syndrome, acid hyposecretion in pancreatic cholera and in somatostatinoma. In these cases, hormonal hypersecretion is directly responsible for the functional disturbances and the related symptoms; 2) gastric pathological conditions are sometimes accompanied by changes in hormonal secretion, but the level of interdependence is variable : high blood gastrin is directly depending upon the atrophic gastritis in pernicious anemia; this mechanism was also suggested in case of gastric carcinoma. Concerning ulcer disease, numerous problems are unsolved in respect to blood gastrin (basal and stimulated) abnormalities, as well as somatostatin and GIP secretions."}
{"id": "PMID:475324", "title": "Immunoglobulin levels and genetic polymorphisms in the Sukuma of Tanzania.", "content": "In a sample of 150 Sukuma from north central Tanzania, immunoglobulin G, M, A, and E levels were studied in relation to the phenotypes in 22 polymorphic systems. No strong associations were detected, though there is a suggestion of an association between IgG and haptoglobin type, with elevation of mean levels in heterozygotes.", "contents": "Immunoglobulin levels and genetic polymorphisms in the Sukuma of Tanzania. In a sample of 150 Sukuma from north central Tanzania, immunoglobulin G, M, A, and E levels were studied in relation to the phenotypes in 22 polymorphic systems. No strong associations were detected, though there is a suggestion of an association between IgG and haptoglobin type, with elevation of mean levels in heterozygotes."}
{"id": "PMID:475325", "title": "Changes in plasma inorganic phosphorus and alkaline phosphatase activity during the adolescent growth spurt.", "content": "Longitudinal data on changes in the concentrations of plasma inorganic phosphorus and plasma alkaline phosphatase activity in 23 girls and 44 boys during the adolescent growth spurt are reported together with the height velocities, ages and sexual maturity ratings. The average age at the peak of the growth spurt was 12.5 years in the girls and 14.1 years in the boys with mean annual height gains of 7.0 and 9.7 cm/year respectively. In both sexes, plasma alkaline phosphatase activity rose and fell with the growth velocity during the growth spurt. Plasma inorganic phosphorus rose to reach a peak in the 4 months before the peak of the growth spurt in height; this rise was statisically significant in the boys but not in the girls. Values subsequently fell rapidly towards the normal adult concentrations. Plasma calcium, total protein, and albumin concentrations were also followed during this time, but were not at any point significantly different from normal adult values. These findings provide a guide for the interpretation of plasma biochemistry in adolescent patients.", "contents": "Changes in plasma inorganic phosphorus and alkaline phosphatase activity during the adolescent growth spurt. Longitudinal data on changes in the concentrations of plasma inorganic phosphorus and plasma alkaline phosphatase activity in 23 girls and 44 boys during the adolescent growth spurt are reported together with the height velocities, ages and sexual maturity ratings. The average age at the peak of the growth spurt was 12.5 years in the girls and 14.1 years in the boys with mean annual height gains of 7.0 and 9.7 cm/year respectively. In both sexes, plasma alkaline phosphatase activity rose and fell with the growth velocity during the growth spurt. Plasma inorganic phosphorus rose to reach a peak in the 4 months before the peak of the growth spurt in height; this rise was statisically significant in the boys but not in the girls. Values subsequently fell rapidly towards the normal adult concentrations. Plasma calcium, total protein, and albumin concentrations were also followed during this time, but were not at any point significantly different from normal adult values. These findings provide a guide for the interpretation of plasma biochemistry in adolescent patients."}
{"id": "PMID:475331", "title": "Red cell adenosine deaminase (ADA) polymorphism in Southern Africa, with special reference to ADA deficiency among the !Kung.", "content": "Studies have been carried out on polymorphism of adenosine deaminase in 36 Southern African populations comprising more than 3000 individuals. The common variant allele ADA2 has been found to attain polymorphic frequencies only in those populations descended from non-indigenous (i.e. non-Negro and non-Khoisan) groups. Its presence in certain other populations at low frequencies could be ascribed to small-scale Caucasoid admixture. A deficiency of the enzyme is found in certain members of the !Kung division of the San ('Bushman'). The low levels of enzyme activity are not associated with severe combined immunodeficiency and the gene which determines them appears to be polymorphic in the !Kung and possibly in some other San populations as well as possibly in Negro populations which have received substantial contributions of San genes.", "contents": "Red cell adenosine deaminase (ADA) polymorphism in Southern Africa, with special reference to ADA deficiency among the !Kung. Studies have been carried out on polymorphism of adenosine deaminase in 36 Southern African populations comprising more than 3000 individuals. The common variant allele ADA2 has been found to attain polymorphic frequencies only in those populations descended from non-indigenous (i.e. non-Negro and non-Khoisan) groups. Its presence in certain other populations at low frequencies could be ascribed to small-scale Caucasoid admixture. A deficiency of the enzyme is found in certain members of the !Kung division of the San ('Bushman'). The low levels of enzyme activity are not associated with severe combined immunodeficiency and the gene which determines them appears to be polymorphic in the !Kung and possibly in some other San populations as well as possibly in Negro populations which have received substantial contributions of San genes."}
{"id": "PMID:475326", "title": "Anthropometric survey of children in rural Khartoum, Sudan.", "content": "The results of a cross-sectional anthropometric survey of Sudanese children up to the age of thirteen years and six months is reported. Children in whom a quick clinical examination revealed pathology were excluded, as were children who did not have a true birth certificate. Height, weight and mid-arm circumference were measured in all children, head and chest circumference were measured in children up to the age of 5 years and 6 months. Skinfold thickness at biceps, triceps, subscapular and suprailiac sites were measured in children above the age of 30 months. The children were found to be shorter and lighter, and to have less subcutaneous fat than British children. The findings are also compared with those from other African populations. Comparison with a survey performed in the same village seven years earlier reveals that the heights and weights of the children have hardly changed despite a considerable improvement in economic status. The children have a lower weight for height than British and other African populations.", "contents": "Anthropometric survey of children in rural Khartoum, Sudan. The results of a cross-sectional anthropometric survey of Sudanese children up to the age of thirteen years and six months is reported. Children in whom a quick clinical examination revealed pathology were excluded, as were children who did not have a true birth certificate. Height, weight and mid-arm circumference were measured in all children, head and chest circumference were measured in children up to the age of 5 years and 6 months. Skinfold thickness at biceps, triceps, subscapular and suprailiac sites were measured in children above the age of 30 months. The children were found to be shorter and lighter, and to have less subcutaneous fat than British children. The findings are also compared with those from other African populations. Comparison with a survey performed in the same village seven years earlier reveals that the heights and weights of the children have hardly changed despite a considerable improvement in economic status. The children have a lower weight for height than British and other African populations."}
{"id": "PMID:475333", "title": "The secondary sex ratio, paternal age, maternal age and birth order in Japan.", "content": "The simultaneous effects of several variables on the secondary sex ratio have been examined using data from over 3.7 million births which occurred in Japan during 1975--6. A weak and negative association between sex ratio and birth order was observed but it was not significant in the statistical sense. A negative effect of paternal age--birth order interaction was obtained when maternal age was controlled. The quadratic model is much more powerful than the linear model in explaining the sex ratio variability.", "contents": "The secondary sex ratio, paternal age, maternal age and birth order in Japan. The simultaneous effects of several variables on the secondary sex ratio have been examined using data from over 3.7 million births which occurred in Japan during 1975--6. A weak and negative association between sex ratio and birth order was observed but it was not significant in the statistical sense. A negative effect of paternal age--birth order interaction was obtained when maternal age was controlled. The quadratic model is much more powerful than the linear model in explaining the sex ratio variability."}
{"id": "PMID:475334", "title": "Cultural and biological determinants of lipoprotein concentrations.", "content": "A general linear model is presented here for biological and cultural inheritance involving ten parameters to be estimated from 16 correlations in nuclear families, providing ample degrees of freedom to test goodness of fit. Applied to six lipoprotein traits the model fits acceptably to all, although there is evidence of transient maternal effects for cholesterol and lipemia. Genetic heritability in children ranges from 0.175 for triglyceride to 0.562 for total cholesterol. Cultural heritability in children ranges from 0.012 for VLDL to 0.149 for HDL-cholesterol.", "contents": "Cultural and biological determinants of lipoprotein concentrations. A general linear model is presented here for biological and cultural inheritance involving ten parameters to be estimated from 16 correlations in nuclear families, providing ample degrees of freedom to test goodness of fit. Applied to six lipoprotein traits the model fits acceptably to all, although there is evidence of transient maternal effects for cholesterol and lipemia. Genetic heritability in children ranges from 0.175 for triglyceride to 0.562 for total cholesterol. Cultural heritability in children ranges from 0.012 for VLDL to 0.149 for HDL-cholesterol."}
{"id": "PMID:475329", "title": "Age at menarche in rural France.", "content": "Data upon age at menarche have been collected among 254 schoolgirls of a relatively endogamous rural population in central France. The median age at menarche estimated by probits was 13.05+/-0.18, SD1.42. This accords well with results obtained from urban samples in the years 1965-68. Both rural and urban samples give a median age 0.5 year earlier than date from a national survey performed in 1950.", "contents": "Age at menarche in rural France. Data upon age at menarche have been collected among 254 schoolgirls of a relatively endogamous rural population in central France. The median age at menarche estimated by probits was 13.05+/-0.18, SD1.42. This accords well with results obtained from urban samples in the years 1965-68. Both rural and urban samples give a median age 0.5 year earlier than date from a national survey performed in 1950."}
{"id": "PMID:475328", "title": "Nutrition and menarche in rural Hyderabad.", "content": "Mean age at menarche was estimated in 739 rural Hyderabad girls aged 12 to 18 years using the status-quo method. These girls had been previously measured and were divided into four groups according to their height at age five. Those who were short at age five had a significantly later menarche (15.2+/-0.13) than those who were tall at age five (13.7+/-0.19). Since 14% of the short-at-five girls had had signs of severe undernutrition and none of the tall had had these, the shortness and late menarche are both attributed to undernutrition.", "contents": "Nutrition and menarche in rural Hyderabad. Mean age at menarche was estimated in 739 rural Hyderabad girls aged 12 to 18 years using the status-quo method. These girls had been previously measured and were divided into four groups according to their height at age five. Those who were short at age five had a significantly later menarche (15.2+/-0.13) than those who were tall at age five (13.7+/-0.19). Since 14% of the short-at-five girls had had signs of severe undernutrition and none of the tall had had these, the shortness and late menarche are both attributed to undernutrition."}
{"id": "PMID:475327", "title": "Harpenden electronic read-out (HERO) skinfold calipers.", "content": "The Harpenden skinfold caliper has been modified so as to give a simple digital display or an output which may be fed directly to more sophisticated equipment (e.g. digital computer or X-Y plotter) for further analysis.", "contents": "Harpenden electronic read-out (HERO) skinfold calipers. The Harpenden skinfold caliper has been modified so as to give a simple digital display or an output which may be fed directly to more sophisticated equipment (e.g. digital computer or X-Y plotter) for further analysis."}
{"id": "PMID:475335", "title": "Family handedness in three generations predicted by the right shift theory.", "content": "The hand preferences of Open University (OU) students and their relatives, including children, are described. As in earlier series, estimates of heritability are higher for mothers than fathers. There is no evidence of smaller heritability for paternal than maternal grandparents. The distribution of left-handedness in families is examined in the light of predictions of the right shift theory and on the assumption that the shift depends on a single gene. Good agreement is found between the observed and expected numbers of R x R, L x R and L x L families. Predictions are successful for both strict and generous criteria of sinistrality. Generation differences are found between OU students and their parents and between the students and their children. These are discussed from the viewpoint of a possible heterozygote advantage in intelligence. The higher proportion of sinistral children born to sinistral mothers than fathers can be partly accounted for by supposing that the right shift is more effective in females than males.", "contents": "Family handedness in three generations predicted by the right shift theory. The hand preferences of Open University (OU) students and their relatives, including children, are described. As in earlier series, estimates of heritability are higher for mothers than fathers. There is no evidence of smaller heritability for paternal than maternal grandparents. The distribution of left-handedness in families is examined in the light of predictions of the right shift theory and on the assumption that the shift depends on a single gene. Good agreement is found between the observed and expected numbers of R x R, L x R and L x L families. Predictions are successful for both strict and generous criteria of sinistrality. Generation differences are found between OU students and their parents and between the students and their children. These are discussed from the viewpoint of a possible heterozygote advantage in intelligence. The higher proportion of sinistral children born to sinistral mothers than fathers can be partly accounted for by supposing that the right shift is more effective in females than males."}
{"id": "PMID:475336", "title": "Choice of ascertainment model II. Discrimination between multi-proband models by means of birth order data.", "content": "Probability models have been developed for family data, where each family has been selected or ascertained through a group of m probands, where m is a known parameter, not a random variable. The birth orders of the probands among the affected children are reported for each family. It is demonstrated that this information is sufficient for choice of ascertainment model. The conditional probability that an ascertained family with s children has r affected ones, depends, in addition to the segregation parameter, on the birth order of the youngest proband only. By means of the joint distribution of the birth orders of the other probands, it can be demonstrated if the ascertainment takes place through affected children near to or distant from each other in relative birth orders. Statistical methods have been developed for cases with two and cases with m probands.", "contents": "Choice of ascertainment model II. Discrimination between multi-proband models by means of birth order data. Probability models have been developed for family data, where each family has been selected or ascertained through a group of m probands, where m is a known parameter, not a random variable. The birth orders of the probands among the affected children are reported for each family. It is demonstrated that this information is sufficient for choice of ascertainment model. The conditional probability that an ascertained family with s children has r affected ones, depends, in addition to the segregation parameter, on the birth order of the youngest proband only. By means of the joint distribution of the birth orders of the other probands, it can be demonstrated if the ascertainment takes place through affected children near to or distant from each other in relative birth orders. Statistical methods have been developed for cases with two and cases with m probands."}
{"id": "PMID:475337", "title": "The 'singles' method for segregation analysis under incomplete ascertainment.", "content": "Li & Mantel (1968) proposed a simple new method of segregation analysis under complete ascertainment. This paper shows that, with a slight modification, the method is also valid under incomplete ascertainment. The assumptions concerning ascertainment are discussed and the method compared with the maximum likelihood method and Weinberg's proband method.", "contents": "The 'singles' method for segregation analysis under incomplete ascertainment. Li & Mantel (1968) proposed a simple new method of segregation analysis under complete ascertainment. This paper shows that, with a slight modification, the method is also valid under incomplete ascertainment. The assumptions concerning ascertainment are discussed and the method compared with the maximum likelihood method and Weinberg's proband method."}
{"id": "PMID:475338", "title": "Isolation by distance: reply to Lalouel and Morton.", "content": "Lalouel's assertion that I misinterpreted Mal\u00e9cot's work on isolation by distance may or may not be correct. If so, my assertions of error in Mal\u00e9cot's derivation are wrong, although they do apply to others who have used models involving a spatial continuum. Lalouel's other claims of error in my derivations of the consequences of a spatially continuous model of population reproduction and migration are incorrect, with the exception of one isolated misprint.", "contents": "Isolation by distance: reply to Lalouel and Morton. Lalouel's assertion that I misinterpreted Mal\u00e9cot's work on isolation by distance may or may not be correct. If so, my assertions of error in Mal\u00e9cot's derivation are wrong, although they do apply to others who have used models involving a spatial continuum. Lalouel's other claims of error in my derivations of the consequences of a spatially continuous model of population reproduction and migration are incorrect, with the exception of one isolated misprint."}
{"id": "PMID:475340", "title": "[Immunochemical properties of cyanogen bromide fragments of human serum albumin (author's transl)].", "content": "Fragments A, B and C obtained by CNBr degradation of human serum albumin (HSA) were studied by specific quantitative precipitation with anti-HSA sera. A co-precipitation has been observed between fragments B and C as well as between C and A which follow each others in the albumin molecule. On the contrary, there was no co-precipitation between fragments B and A which correspond respectively to the N- and C-terminal part of the albumin molecule. Moreover, using inhibition of passive haemagglutination, it has been observed that fragment A does not inhibit anti-B antibodies and fragment B does not inhibit anti-A antibodies. These results indicate that there are common antigenic sites to fragments B and C as well as to C and A but not to B and A. This is in agreement with the data upon the structure of HSA showing that it is made of three domains in linear sequence.", "contents": "[Immunochemical properties of cyanogen bromide fragments of human serum albumin (author's transl)]. Fragments A, B and C obtained by CNBr degradation of human serum albumin (HSA) were studied by specific quantitative precipitation with anti-HSA sera. A co-precipitation has been observed between fragments B and C as well as between C and A which follow each others in the albumin molecule. On the contrary, there was no co-precipitation between fragments B and A which correspond respectively to the N- and C-terminal part of the albumin molecule. Moreover, using inhibition of passive haemagglutination, it has been observed that fragment A does not inhibit anti-B antibodies and fragment B does not inhibit anti-A antibodies. These results indicate that there are common antigenic sites to fragments B and C as well as to C and A but not to B and A. This is in agreement with the data upon the structure of HSA showing that it is made of three domains in linear sequence."}
{"id": "PMID:475341", "title": "A study of the breakdown of the third component of human complement (C3).", "content": "This paper reports the description of fragments of human C3, purified from aged sera. Sera were incubated at different temperatures (4 degrees, 20 degrees and 37 degrees C) for 8 days or more. At 4 degrees C, the major isolated fragment was C3b which showed a heterogeneity of structure. Under reducing conditions, it liberated a 40,000 dalton fragment which reacted against an anti-C3d and which we called \"C3d-like\". At 20 degrees C, C3b was partially destroyed to yield the C3c- and C3d-fragments. At 37 degrees C, C3d and C3c were obtained. C3c presented a structural heterogeneity. It was made either of two chains of 75,000 daltons or of three chains with 75,000, 38,000 and 31,000 dalton MW. The comparison of these fragments with those obtained by tryptic hydrolysis led us to propose a pathway to the degradation of C3 and to bring some informations about the structure of this molecule.", "contents": "A study of the breakdown of the third component of human complement (C3). This paper reports the description of fragments of human C3, purified from aged sera. Sera were incubated at different temperatures (4 degrees, 20 degrees and 37 degrees C) for 8 days or more. At 4 degrees C, the major isolated fragment was C3b which showed a heterogeneity of structure. Under reducing conditions, it liberated a 40,000 dalton fragment which reacted against an anti-C3d and which we called \"C3d-like\". At 20 degrees C, C3b was partially destroyed to yield the C3c- and C3d-fragments. At 37 degrees C, C3d and C3c were obtained. C3c presented a structural heterogeneity. It was made either of two chains of 75,000 daltons or of three chains with 75,000, 38,000 and 31,000 dalton MW. The comparison of these fragments with those obtained by tryptic hydrolysis led us to propose a pathway to the degradation of C3 and to bring some informations about the structure of this molecule."}
{"id": "PMID:475342", "title": "[Influence of the irritant used to provoke the peritoneal exudate used in the macrophage migration inhibition test (author's transl)].", "content": "The effect of the irritant chosen to provoke the peritoneal exudate in donors of cells for the in vitro migration inhibition test was studied. Guinea-pigs with delayed hypersensitivity to tubercle bacilli were used. Irritants studied included starch, Bayol and thioglycollate medium. The exudates obtained with thioglycollate were found to show greater inhibition than those obtained with the other irritants in the in vitro response to PPD, Inhibition of migration was seen when 1 microgram/ml of PPD was added to the culture medium; with this dose the inhibition of migration was 53%. The state of activation of macrophages caused by the irritant thus appears to have some importance for their responsiveness to factors liberated by specifically sensitized lymphocytes.", "contents": "[Influence of the irritant used to provoke the peritoneal exudate used in the macrophage migration inhibition test (author's transl)]. The effect of the irritant chosen to provoke the peritoneal exudate in donors of cells for the in vitro migration inhibition test was studied. Guinea-pigs with delayed hypersensitivity to tubercle bacilli were used. Irritants studied included starch, Bayol and thioglycollate medium. The exudates obtained with thioglycollate were found to show greater inhibition than those obtained with the other irritants in the in vitro response to PPD, Inhibition of migration was seen when 1 microgram/ml of PPD was added to the culture medium; with this dose the inhibition of migration was 53%. The state of activation of macrophages caused by the irritant thus appears to have some importance for their responsiveness to factors liberated by specifically sensitized lymphocytes."}
{"id": "PMID:475348", "title": "Homozygous expression of a dominant gene for Charcot-Marie-Tooth neuropathy.", "content": "A kindred of 68 French Acadians who were heterozygous for a dominant gene of Charcot-Marie-Tooth disease associated with peripheral nerve hypertrophy are described. Marriage between 2 heterozygotes resulted in 2 homozygous offspring. Clinical features of the homozygotes were similar to the classic description of Dejerine-Sottas disease. Laboratory studies in this family revealed no chemical, metabolic, or chromosomal abnormalities in either the homozygotes or the heterozygotes.", "contents": "Homozygous expression of a dominant gene for Charcot-Marie-Tooth neuropathy. A kindred of 68 French Acadians who were heterozygous for a dominant gene of Charcot-Marie-Tooth disease associated with peripheral nerve hypertrophy are described. Marriage between 2 heterozygotes resulted in 2 homozygous offspring. Clinical features of the homozygotes were similar to the classic description of Dejerine-Sottas disease. Laboratory studies in this family revealed no chemical, metabolic, or chromosomal abnormalities in either the homozygotes or the heterozygotes."}
{"id": "PMID:475349", "title": "Degeneration of the cerebellar system in X-chromosome-linked copper malabsorption.", "content": "Examination of the cerebellar system of 5 autopsied patients with X-chromosome-linked copper malabsorption led to the discovery that among cerebellar afferent systems, only the dorsal spinocerebellar tract showed consistent degenerative changes. Cerebellar cortical lesions comprised granule cell loss and Purkinje cell abnormalities that included nuclear and cytoplasmic degenerative changes with cell loss and deficient dendritic arborization as well as the presence of somatic sprouts. Perisomatic baskets were often absent in the area of sprouting Purkinje cells. Cerebellar cortical topographical analysis revealed relative sparing of the caudal portion of the cerebellum. The nodulus was intact in all patients. The dentate nucleus and the superior cerebellar peduncle were preserved. The red nucleus was consistently degenerated.", "contents": "Degeneration of the cerebellar system in X-chromosome-linked copper malabsorption. Examination of the cerebellar system of 5 autopsied patients with X-chromosome-linked copper malabsorption led to the discovery that among cerebellar afferent systems, only the dorsal spinocerebellar tract showed consistent degenerative changes. Cerebellar cortical lesions comprised granule cell loss and Purkinje cell abnormalities that included nuclear and cytoplasmic degenerative changes with cell loss and deficient dendritic arborization as well as the presence of somatic sprouts. Perisomatic baskets were often absent in the area of sprouting Purkinje cells. Cerebellar cortical topographical analysis revealed relative sparing of the caudal portion of the cerebellum. The nodulus was intact in all patients. The dentate nucleus and the superior cerebellar peduncle were preserved. The red nucleus was consistently degenerated."}
{"id": "PMID:475344", "title": "Synthesis of antibodies and immunoglobulins bearing recipient allotypic markers and donor idiotypic specificities in irradiated rabbits grafted with allogeneic cells from hyperimmune donors.", "content": "Irradiated rabbits were grafted with a mixture of bone marrow, lymph node and spleen cells from donors hyperimmunized against TMV. Recipient and donors were characterized by different a allotypic specificities. Antibodies synthesized in the recipients display allotypic markers from the recipients but idiotypic specificities cross-reactive with those of donor antibodies. The results show that the differentiation of new host B cells is influenced by the presence of donor memory cells and are interpreted in the light of network concepts.", "contents": "Synthesis of antibodies and immunoglobulins bearing recipient allotypic markers and donor idiotypic specificities in irradiated rabbits grafted with allogeneic cells from hyperimmune donors. Irradiated rabbits were grafted with a mixture of bone marrow, lymph node and spleen cells from donors hyperimmunized against TMV. Recipient and donors were characterized by different a allotypic specificities. Antibodies synthesized in the recipients display allotypic markers from the recipients but idiotypic specificities cross-reactive with those of donor antibodies. The results show that the differentiation of new host B cells is influenced by the presence of donor memory cells and are interpreted in the light of network concepts."}
{"id": "PMID:475350", "title": "Endoneurial fluid pressure in wallerian degeneration.", "content": "Endoneurial fluid pressure (EFP) was recorded by an active, servo-null pressure system after a glass micropipette was inserted into rat sciatic nerve undergoing wallerian degeneration. The lesions were produced by crushing the left sciatic nerve of the anesthetized animal at its point of entry into the thigh. Eighty-four animals were employed in this experiment, in which EFP was recorded from sham-operated rats and other controls as well as from rats with wallerian degeneration. The experiment was designed so that EFP could be recorded from 2 or more experimental animals at daily intervals starting at day 0 and concluding on day 28. Pressure progressively increased during the first week, reaching a peak elevation four to five times normal. The subsequent decline in EFP was more gradual, with values approaching normal during the third week after injury. Linear regression analysis showed the progressive increase in EFP to be statistically significant (p less than or equal to 0.01). To determine the time at which EFP was maximum, we used the Marquardt computer algorithm for lease-squares estimation of nonlinear variables. By this procedure the peak value for EFP occurred at six days. These biophysical observations were correlated with subsequent microscopic examination of 1 mu thick sections of Araldite-embedded sciatic nerve. Microscopy confirmed the presence of wallerian degeneration associated with edema, which was observed in every instance of elevated EFP.", "contents": "Endoneurial fluid pressure in wallerian degeneration. Endoneurial fluid pressure (EFP) was recorded by an active, servo-null pressure system after a glass micropipette was inserted into rat sciatic nerve undergoing wallerian degeneration. The lesions were produced by crushing the left sciatic nerve of the anesthetized animal at its point of entry into the thigh. Eighty-four animals were employed in this experiment, in which EFP was recorded from sham-operated rats and other controls as well as from rats with wallerian degeneration. The experiment was designed so that EFP could be recorded from 2 or more experimental animals at daily intervals starting at day 0 and concluding on day 28. Pressure progressively increased during the first week, reaching a peak elevation four to five times normal. The subsequent decline in EFP was more gradual, with values approaching normal during the third week after injury. Linear regression analysis showed the progressive increase in EFP to be statistically significant (p less than or equal to 0.01). To determine the time at which EFP was maximum, we used the Marquardt computer algorithm for lease-squares estimation of nonlinear variables. By this procedure the peak value for EFP occurred at six days. These biophysical observations were correlated with subsequent microscopic examination of 1 mu thick sections of Araldite-embedded sciatic nerve. Microscopy confirmed the presence of wallerian degeneration associated with edema, which was observed in every instance of elevated EFP."}
{"id": "PMID:475346", "title": "[Equal sensitivity of normal or tumoral fibroblasts to cytotoxic and cytostatic effects of normal or \"Corynebacterium parvum\" activated peritoneal exudate cells (author's transl)].", "content": "Cytotoxic and cytostatic properties of peritoneal exudate cells from BALB/c mice either normal or pretreated with Corynebacterium parvum normal were studied using as target cells two lines of BALB/c fibroblasts one normal (BALB) and one transformed by Kirsten virus (K.BALB).. C. parvum activated cells displayed higher cytotoxic as measured by 51Cr or 3H-thymidine release and cytostatic, as measured by 3H-thymidine incorporation, activities against these lines of syngeneic cells. Cytotoxic effect of normal peritoneal cells was evident when 51Cr or 3H-thymidine release was used whereas no such effect was found when cytolytic plaque formation test was used. Sensitivity of both BALB and K.BALB target cell lines was found to be equal to cytotoxic and cytostatic activities of C. parvum activated or normal syngeneic peritoneal cells.", "contents": "[Equal sensitivity of normal or tumoral fibroblasts to cytotoxic and cytostatic effects of normal or \"Corynebacterium parvum\" activated peritoneal exudate cells (author's transl)]. Cytotoxic and cytostatic properties of peritoneal exudate cells from BALB/c mice either normal or pretreated with Corynebacterium parvum normal were studied using as target cells two lines of BALB/c fibroblasts one normal (BALB) and one transformed by Kirsten virus (K.BALB).. C. parvum activated cells displayed higher cytotoxic as measured by 51Cr or 3H-thymidine release and cytostatic, as measured by 3H-thymidine incorporation, activities against these lines of syngeneic cells. Cytotoxic effect of normal peritoneal cells was evident when 51Cr or 3H-thymidine release was used whereas no such effect was found when cytolytic plaque formation test was used. Sensitivity of both BALB and K.BALB target cell lines was found to be equal to cytotoxic and cytostatic activities of C. parvum activated or normal syngeneic peritoneal cells."}
{"id": "PMID:475351", "title": "Determinants of survival in children with brain tumors.", "content": "A study was conducted in metropolitan Baltimore to examine time trends in survival and factors which influence survival of children with brain tumors. One hundred eighty-four cases diagnosed from 1960 to 1975 were identified; 76.5% occurred in white children and 48% were girls. Five-year survival rates were 35% for children diagnosed in 1960-64, 34% in 1965-69, and 49% in 1970-74. Girls had consistently better survival rates than boys. White children experienced consistently better survival rates than blacks. Of those who survived the first year, children with supratentorial tumors had better five-year survival rates (46%) than those which with infratentorial tumors (27%). Of those who survived the first year, children with astrocytomas had better survival rates than those with other types of tumors.", "contents": "Determinants of survival in children with brain tumors. A study was conducted in metropolitan Baltimore to examine time trends in survival and factors which influence survival of children with brain tumors. One hundred eighty-four cases diagnosed from 1960 to 1975 were identified; 76.5% occurred in white children and 48% were girls. Five-year survival rates were 35% for children diagnosed in 1960-64, 34% in 1965-69, and 49% in 1970-74. Girls had consistently better survival rates than boys. White children experienced consistently better survival rates than blacks. Of those who survived the first year, children with supratentorial tumors had better five-year survival rates (46%) than those which with infratentorial tumors (27%). Of those who survived the first year, children with astrocytomas had better survival rates than those with other types of tumors."}
{"id": "PMID:475352", "title": "Spinal fluid aluminum levels in patients with Alzheimer disease.", "content": "Spinal fluid from 180 male patients was examined for aluminum levels. Values were coordinated for age and protein determinations. In addition, values were listed according to the patient's diagnosis. Although in most instances no correlation could be made, 10 patients with Alzheimer disease showed significantly low levels of aluminum.", "contents": "Spinal fluid aluminum levels in patients with Alzheimer disease. Spinal fluid from 180 male patients was examined for aluminum levels. Values were coordinated for age and protein determinations. In addition, values were listed according to the patient's diagnosis. Although in most instances no correlation could be made, 10 patients with Alzheimer disease showed significantly low levels of aluminum."}
{"id": "PMID:475347", "title": "Ia versus K/D antigens in immunological enhancement of tumour allografts. II. Studies with alloimmune sera prepared in recombinant strains.", "content": "Previous studies have demonstrated that anti-K/D antibodies obtained by specific absorption on and elution from relevant red blood cells were capable of inducing enhancement of tumour cells in allogeneic hosts. Anti-Ia antibodies were not found enhancing except when alloimmune sera prepared by skin grafts were used. In the present report, anti-H-2K and anti-H-2D or anti-H-2I sera were obtained in mouse recombinant strains, in order to obviate such absorption and elution procedures. It has been shown that SaI-tumour cells (H-2a, i.e. H-2k/d) can be enhanced in allogeneic recipients, CBA (H-2k) or C57BL/Ks (H-2d) by antisera directed towards H-2Dd or H-2Kk. Alloimmune sera directed towards H2i-coded antigens prepared by immunization with spleen cells had no enhancing activity on the same tumour allografts in the CBA-A/J strain combination anti-IC'D,Sd,Gd) but caused a transitory effect in the C57BL/Ks-A/J combination (anti-IA'K,IBk,IJk,IEk).", "contents": "Ia versus K/D antigens in immunological enhancement of tumour allografts. II. Studies with alloimmune sera prepared in recombinant strains. Previous studies have demonstrated that anti-K/D antibodies obtained by specific absorption on and elution from relevant red blood cells were capable of inducing enhancement of tumour cells in allogeneic hosts. Anti-Ia antibodies were not found enhancing except when alloimmune sera prepared by skin grafts were used. In the present report, anti-H-2K and anti-H-2D or anti-H-2I sera were obtained in mouse recombinant strains, in order to obviate such absorption and elution procedures. It has been shown that SaI-tumour cells (H-2a, i.e. H-2k/d) can be enhanced in allogeneic recipients, CBA (H-2k) or C57BL/Ks (H-2d) by antisera directed towards H-2Dd or H-2Kk. Alloimmune sera directed towards H2i-coded antigens prepared by immunization with spleen cells had no enhancing activity on the same tumour allografts in the CBA-A/J strain combination anti-IC'D,Sd,Gd) but caused a transitory effect in the C57BL/Ks-A/J combination (anti-IA'K,IBk,IJk,IEk)."}
{"id": "PMID:475353", "title": "Familial arteriovenous malformation.", "content": "A family is reported in which 3 siblings, 2 with the same and the third with a different father, were demonstrated to have cerebral arteriovenous malformations.", "contents": "Familial arteriovenous malformation. A family is reported in which 3 siblings, 2 with the same and the third with a different father, were demonstrated to have cerebral arteriovenous malformations."}
{"id": "PMID:475354", "title": "Hypernatremic hemorrhagic encephalopathy.", "content": "A 12-year-old juvenile diabetic was inadvertently given 500 ml of hypertonic saline intravenously. He developed hypernatremia, hyperosmolality, metabolic acidosis, and hyperglycemia. Seizures and stupor ensued, followed by coma and death. Computerized cranial tomography revealed numerous small subcortical hemorrhages that were verified postmortem.", "contents": "Hypernatremic hemorrhagic encephalopathy. A 12-year-old juvenile diabetic was inadvertently given 500 ml of hypertonic saline intravenously. He developed hypernatremia, hyperosmolality, metabolic acidosis, and hyperglycemia. Seizures and stupor ensued, followed by coma and death. Computerized cranial tomography revealed numerous small subcortical hemorrhages that were verified postmortem."}
{"id": "PMID:475358", "title": "Mechanism and specificity of action of ribavirin.", "content": "Ribavirin at a concentration of 30 mug/ml added immediately after infection completely inhibited influenza A/Port Chalmers/1/73 (H(3)N(2)) virus hemagglutinin production in infected MDCK cells. Under these conditions, host cell protein synthesis was inhibited by only 10 to 20%. Polyacrylamide gel electrophoresis of [(35)S]methionine-labeled material from virus-infected cultures confirmed that ribavirin inhibited viral but not host cell protein synthesis. In parallel experiments, actinomycin D also preferentially inhibited viral protein synthesis. The possibility that ribavirin inhibited viral protein synthesis as a result of general inhibition of ribonucleic acid synthesis was therefore examined. In uninfected cells, ribavirin at 30 mug/ml inhibited the incorporation of [(14)C]inosine or [(3)H]uridine into ribonucleic acid but stimulated the incorporation of [(3)H]guanosine. The effects noted are consistent with an inhibition of the host cell enzyme inosine 5'-monophosphate dehydrogenase. This suggestion is supported by the finding that addition of guanosine, but not inosine, to the culture medium substantially reversed the antiviral effect of ribavirin. There was no separation between the concentration of ribavirin causing inhibition of influenza A viral protein synthesis or inhibition of MDCK cell ribonucleic acid synthesis, suggesting that ribavirin is not specifically antiviral in this system but inhibits viral protein synthesis as a result of the general inhibition of ribonucleic acid synthesis.", "contents": "Mechanism and specificity of action of ribavirin. Ribavirin at a concentration of 30 mug/ml added immediately after infection completely inhibited influenza A/Port Chalmers/1/73 (H(3)N(2)) virus hemagglutinin production in infected MDCK cells. Under these conditions, host cell protein synthesis was inhibited by only 10 to 20%. Polyacrylamide gel electrophoresis of [(35)S]methionine-labeled material from virus-infected cultures confirmed that ribavirin inhibited viral but not host cell protein synthesis. In parallel experiments, actinomycin D also preferentially inhibited viral protein synthesis. The possibility that ribavirin inhibited viral protein synthesis as a result of general inhibition of ribonucleic acid synthesis was therefore examined. In uninfected cells, ribavirin at 30 mug/ml inhibited the incorporation of [(14)C]inosine or [(3)H]uridine into ribonucleic acid but stimulated the incorporation of [(3)H]guanosine. The effects noted are consistent with an inhibition of the host cell enzyme inosine 5'-monophosphate dehydrogenase. This suggestion is supported by the finding that addition of guanosine, but not inosine, to the culture medium substantially reversed the antiviral effect of ribavirin. There was no separation between the concentration of ribavirin causing inhibition of influenza A viral protein synthesis or inhibition of MDCK cell ribonucleic acid synthesis, suggesting that ribavirin is not specifically antiviral in this system but inhibits viral protein synthesis as a result of the general inhibition of ribonucleic acid synthesis."}
{"id": "PMID:475359", "title": "Antimicrobial susceptibility testing of Mycobacterium fortuitum complex.", "content": "A total of 24 strains of the Mycobacterium fortuitum complex were tested for susceptibility to antimicrobial agents by the disk diffusion and agar dilution techniques. By comparing zones of inhibition obtained with the disk diffusion technique with results of minimal inhibitory concentration determinations, it was shown that disk diffusion results could predict in vitro susceptibility to selected antimicrobial agents. All of 17 strains of M. fortuitum were susceptible to </=1 mug of amikacin per ml. The corresponding average zone of inhibition around a 10-mug amikacin disk was 37 mm. Seven M. chelonei strains were more resistant to amikacin, with minimal inhibitory concentrations ranging from 1 to 32 mug/ml, and the corresponding average zone size was 21 mm. Susceptibility of both M. fortuitum and M. chelonei to tetracycline was variable and none of the M. chelonei strains was inhibited by polymyxin B, whereas M. fortuitum strains consistently had zones of inhibition around the polymyxin disk. It appears that identification to species of the M. fortuitum complex may be of importance with regard to antibiotic susceptibility. Separation of M. fortuitum and M. chelonei was readily accomplished in the present study by the nitrate reduction and 3-day arylsulfatase tests.", "contents": "Antimicrobial susceptibility testing of Mycobacterium fortuitum complex. A total of 24 strains of the Mycobacterium fortuitum complex were tested for susceptibility to antimicrobial agents by the disk diffusion and agar dilution techniques. By comparing zones of inhibition obtained with the disk diffusion technique with results of minimal inhibitory concentration determinations, it was shown that disk diffusion results could predict in vitro susceptibility to selected antimicrobial agents. All of 17 strains of M. fortuitum were susceptible to </=1 mug of amikacin per ml. The corresponding average zone of inhibition around a 10-mug amikacin disk was 37 mm. Seven M. chelonei strains were more resistant to amikacin, with minimal inhibitory concentrations ranging from 1 to 32 mug/ml, and the corresponding average zone size was 21 mm. Susceptibility of both M. fortuitum and M. chelonei to tetracycline was variable and none of the M. chelonei strains was inhibited by polymyxin B, whereas M. fortuitum strains consistently had zones of inhibition around the polymyxin disk. It appears that identification to species of the M. fortuitum complex may be of importance with regard to antibiotic susceptibility. Separation of M. fortuitum and M. chelonei was readily accomplished in the present study by the nitrate reduction and 3-day arylsulfatase tests."}
{"id": "PMID:475360", "title": "Characteristics of herpesvirus mutants resistant to phosphonoformate and phosphonoacetate.", "content": "Mutants of herpes simplex virus type 1, resistant to phosphonoformate (PFA) and phosphonoacetate (PAA), have been selected in cell culture. The PFA-resistant mutant (HSV-PFA(r)) and the PAA-resistant mutant (HSV-PAA(r)) were also resistant to PAA and PFA, respectively. This cross-resistance indicates that PFA and PAA interact at the same site. The HSV-PFA(r) had a decreased susceptibility to vidarabine, but no difference in sensitivity to idoxuridine (5-iodo-2'-deoxyuridine) was observed when compared to the wild type. The induced deoxyribonucleic acid polymerases isolated from cells infected with HSV-PFA(r) and HSV-PAA(r) were both cross-resistant to inhibition by PFA and PAA. No increased resistance to vidarabine triphosphate could be observed, but the susceptibility to inhibition by pyrophosphate was about two times lower. None of the mutants showed any increased temperature sensitivity", "contents": "Characteristics of herpesvirus mutants resistant to phosphonoformate and phosphonoacetate. Mutants of herpes simplex virus type 1, resistant to phosphonoformate (PFA) and phosphonoacetate (PAA), have been selected in cell culture. The PFA-resistant mutant (HSV-PFA(r)) and the PAA-resistant mutant (HSV-PAA(r)) were also resistant to PAA and PFA, respectively. This cross-resistance indicates that PFA and PAA interact at the same site. The HSV-PFA(r) had a decreased susceptibility to vidarabine, but no difference in sensitivity to idoxuridine (5-iodo-2'-deoxyuridine) was observed when compared to the wild type. The induced deoxyribonucleic acid polymerases isolated from cells infected with HSV-PFA(r) and HSV-PAA(r) were both cross-resistant to inhibition by PFA and PAA. No increased resistance to vidarabine triphosphate could be observed, but the susceptibility to inhibition by pyrophosphate was about two times lower. None of the mutants showed any increased temperature sensitivity"}
{"id": "PMID:475361", "title": "Rapid antimicrobial susceptibility test using tetrazolium reduction.", "content": "A rapid method of antimicrobial susceptibility testing has been developed, which uses a modified microdilution procedure and an inoculum of 10(7) bacteria per ml. Results are determined within 4 h with an indicator consisting of 2(p-iodophenyl)-3(p-nitrophenyl)-5-phenyltetrazolium chloride. The precipitation of a red formazan by bacteria uninhibited by antimicrobials is accelerated by the addition of phenazine methosulfate. Isolates are classified as resistant, indeterminate, or susceptible, based on growth in up to two antimicrobial concentrations which conform closely to concentrations which correlate with the millimeter breakpoints used in the Bauer-Kirby method. Results of testing 10 antimicrobial agents against 1,126 isolates were compared with results obtained when the Bauer-Kirby method and the agar dilution procedure were used as reference methods. Enterococci were excluded because of false resistance. Discrepancies were classified as very major (false susceptibility), major (false resistance), and minor (combinations of susceptibility or resistance with indeterminate results). The rapid method versus the agar dilution method yielded 2.3% very major, 0.7% major, and 2.9% minor discrepancies, for a total of 6.0%. Of 58 organism-antimicrobial agent combinations tested, 23 displayed 1% very major discrepancies between the rapid method and the agar dilution method. Six were not therapeutically important. The remainder involved Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter sp., and most organisms tested with chloramphenicol. It is suggested that adjustments in antibiotic concentrations and/or inoculum size may eliminate these discrepancies. The rapid method appeared economical when compared with Autobac 1 and the Bauer-Kirby procedure.", "contents": "Rapid antimicrobial susceptibility test using tetrazolium reduction. A rapid method of antimicrobial susceptibility testing has been developed, which uses a modified microdilution procedure and an inoculum of 10(7) bacteria per ml. Results are determined within 4 h with an indicator consisting of 2(p-iodophenyl)-3(p-nitrophenyl)-5-phenyltetrazolium chloride. The precipitation of a red formazan by bacteria uninhibited by antimicrobials is accelerated by the addition of phenazine methosulfate. Isolates are classified as resistant, indeterminate, or susceptible, based on growth in up to two antimicrobial concentrations which conform closely to concentrations which correlate with the millimeter breakpoints used in the Bauer-Kirby method. Results of testing 10 antimicrobial agents against 1,126 isolates were compared with results obtained when the Bauer-Kirby method and the agar dilution procedure were used as reference methods. Enterococci were excluded because of false resistance. Discrepancies were classified as very major (false susceptibility), major (false resistance), and minor (combinations of susceptibility or resistance with indeterminate results). The rapid method versus the agar dilution method yielded 2.3% very major, 0.7% major, and 2.9% minor discrepancies, for a total of 6.0%. Of 58 organism-antimicrobial agent combinations tested, 23 displayed 1% very major discrepancies between the rapid method and the agar dilution method. Six were not therapeutically important. The remainder involved Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter sp., and most organisms tested with chloramphenicol. It is suggested that adjustments in antibiotic concentrations and/or inoculum size may eliminate these discrepancies. The rapid method appeared economical when compared with Autobac 1 and the Bauer-Kirby procedure."}
{"id": "PMID:475362", "title": "Therapy of experimental herpes simplex encephalitis with aciclovir in mice.", "content": "This report is concerned with the capacities of aciclovir to protect mice challenged intracerebrally with multiple lethal doses of type 1 herpes simplex virus and to control multiplication of this virus in the brain. With treatment initiated 12 h after inoculation and continued for 4 consecutive days, aciclovir administered subcutaneously in daily doses ranging from 40 to 100 mg/kg led to 21-day survival rates of from 33 to 73% and reduced virus titers by 1 to (1/2) x 4 logs on postchallenge day 8. The therapeutic accomplishments of the 100-mg/kg doses of aciclovir were comparable to those of 1,000-mg/kg doses of vidarabine (9-beta-d-arabinofuranosyladenine); however, as measured by impact on body weight, aciclovir was better tolerated than vidarabine at these similarly effective doses.", "contents": "Therapy of experimental herpes simplex encephalitis with aciclovir in mice. This report is concerned with the capacities of aciclovir to protect mice challenged intracerebrally with multiple lethal doses of type 1 herpes simplex virus and to control multiplication of this virus in the brain. With treatment initiated 12 h after inoculation and continued for 4 consecutive days, aciclovir administered subcutaneously in daily doses ranging from 40 to 100 mg/kg led to 21-day survival rates of from 33 to 73% and reduced virus titers by 1 to (1/2) x 4 logs on postchallenge day 8. The therapeutic accomplishments of the 100-mg/kg doses of aciclovir were comparable to those of 1,000-mg/kg doses of vidarabine (9-beta-d-arabinofuranosyladenine); however, as measured by impact on body weight, aciclovir was better tolerated than vidarabine at these similarly effective doses."}
{"id": "PMID:475363", "title": "Relationship between aminoglycoside-induced nephrotoxicity and auditory toxicity.", "content": "We have reviewed our data from 391 patients entered into three prospective, double-blind studies of aminoglycosides and evaluated 127 cases to determine whether aminoglycoside-induced auditory toxicity and nephrotoxicity are independent events. The cases selected for evaluation included all patients treated for greater than 3 days (mean, 7.7 days) who had serial creatinine determinations and were able to cooperate with serial bedside audiograms (250 to 8,000 Hz). Patients received either gentamicin, tobramycin, or amikacin. Drug dosage was altered to keep serum levels 1 h after administration between 5 and 10 mug/ml (gentamicin or tobramycin) or 20 and 40 mug/ml (amikacin). The investigators evaluating auditory toxicity and nephrotoxicity were blind to the aminoglycoside being administered. The incidence of auditory toxicity in the nephrotoxic group (18.2%) was not significantly different from that in the nonnephrotoxic group (15.2%) (P = 0.75; Fisher exact test). There was no statistical difference between the nephrotoxic and auditory toxic groups in patient age, total dose of aminoglycoside, initial creatinine level, duration of therapy, or concurrent use of furosemide or cephalothin. We conclude that aminoglycoside-induced auditory toxicity and nephrotoxicity are independent events when the drug is administered for approximately 7 days and when aminoglycoside levels are maintained within a predefined range.", "contents": "Relationship between aminoglycoside-induced nephrotoxicity and auditory toxicity. We have reviewed our data from 391 patients entered into three prospective, double-blind studies of aminoglycosides and evaluated 127 cases to determine whether aminoglycoside-induced auditory toxicity and nephrotoxicity are independent events. The cases selected for evaluation included all patients treated for greater than 3 days (mean, 7.7 days) who had serial creatinine determinations and were able to cooperate with serial bedside audiograms (250 to 8,000 Hz). Patients received either gentamicin, tobramycin, or amikacin. Drug dosage was altered to keep serum levels 1 h after administration between 5 and 10 mug/ml (gentamicin or tobramycin) or 20 and 40 mug/ml (amikacin). The investigators evaluating auditory toxicity and nephrotoxicity were blind to the aminoglycoside being administered. The incidence of auditory toxicity in the nephrotoxic group (18.2%) was not significantly different from that in the nonnephrotoxic group (15.2%) (P = 0.75; Fisher exact test). There was no statistical difference between the nephrotoxic and auditory toxic groups in patient age, total dose of aminoglycoside, initial creatinine level, duration of therapy, or concurrent use of furosemide or cephalothin. We conclude that aminoglycoside-induced auditory toxicity and nephrotoxicity are independent events when the drug is administered for approximately 7 days and when aminoglycoside levels are maintained within a predefined range."}
{"id": "PMID:475364", "title": "In vitro activity of furazlocillin (Bay k 4999) compared with those of mezlocillin, piperacillin, and standard beta-lactam antibiotics.", "content": "The activity of furazlocillin (Bay k 4999) was compared with those of mezlocillin, piperacillin, and standard beta-lactam antibiotics against a number of gram-positive and gram-negative organisms. These new expanded-spectrum penicillins were less active than penicillin G against most gram-positive organisms. Furazlocillin, mezlocillin, and piperacillin showed activity comparable to ampicillin and penicillin G against Haemophilus influenzae and penicillin-susceptible neisseriae, respectively. None of the drugs tested was effective against penicillin-resistant gonococci. The activity of furazlocillin was greater than that of mezlocillin, piperacillin, ampicillin, or carbenicillin against many Enterobacteriaceae. However, certain beta-lactam-resistant strains among these organisms were not highly susceptible to any of the three new penicillins. Furazlocillin was less active than piperacillin against Pseudomonas aeruginosa but was more active than carbenicillin or mezlocillin. Inoculum effects and discrepancies between minimal inhibitory concentrations and minimal bactericidal concentrations were observed with furazlocillin, mezlocillin, and piperacillin against several genera. The kinetics of bacterial killing by the new penicillins were often slow and incomplete over 24 h, especially in tests with Enterobacter and P. aeruginosa. Synergy was demonstrated between furazlocillin and aminoglycosides against a variety of gram-negative bacilli and Streptococcus faecalis.", "contents": "In vitro activity of furazlocillin (Bay k 4999) compared with those of mezlocillin, piperacillin, and standard beta-lactam antibiotics. The activity of furazlocillin (Bay k 4999) was compared with those of mezlocillin, piperacillin, and standard beta-lactam antibiotics against a number of gram-positive and gram-negative organisms. These new expanded-spectrum penicillins were less active than penicillin G against most gram-positive organisms. Furazlocillin, mezlocillin, and piperacillin showed activity comparable to ampicillin and penicillin G against Haemophilus influenzae and penicillin-susceptible neisseriae, respectively. None of the drugs tested was effective against penicillin-resistant gonococci. The activity of furazlocillin was greater than that of mezlocillin, piperacillin, ampicillin, or carbenicillin against many Enterobacteriaceae. However, certain beta-lactam-resistant strains among these organisms were not highly susceptible to any of the three new penicillins. Furazlocillin was less active than piperacillin against Pseudomonas aeruginosa but was more active than carbenicillin or mezlocillin. Inoculum effects and discrepancies between minimal inhibitory concentrations and minimal bactericidal concentrations were observed with furazlocillin, mezlocillin, and piperacillin against several genera. The kinetics of bacterial killing by the new penicillins were often slow and incomplete over 24 h, especially in tests with Enterobacter and P. aeruginosa. Synergy was demonstrated between furazlocillin and aminoglycosides against a variety of gram-negative bacilli and Streptococcus faecalis."}
{"id": "PMID:475365", "title": "Correlation of netilmicin disk diffusion susceptibility and agar dilution susceptibility.", "content": "The minimal inhibitory concentration of netilmicin determined by the agar dilution method was correlated with the disk diffusion zone of inhibition against 322 clinical isolates. Regression line analysis revealed good correlation, r = -0.90, and suggested that isolates with zone sizes </=14 mm should be considered resistant.", "contents": "Correlation of netilmicin disk diffusion susceptibility and agar dilution susceptibility. The minimal inhibitory concentration of netilmicin determined by the agar dilution method was correlated with the disk diffusion zone of inhibition against 322 clinical isolates. Regression line analysis revealed good correlation, r = -0.90, and suggested that isolates with zone sizes </=14 mm should be considered resistant."}
{"id": "PMID:475366", "title": "Comparative pharmacokinetics of cephalexin, cefaclor, cefadroxil, and CGP 9000.", "content": "In a randomized crossover study, the pharmacokinetics of three new cephalosporin antibiotics, cefaclor, cefadroxil, and CGP 9000, in comparison to cephalexin, were determined after oral administration, by capsules, of 1,000 mg on an empty stomach in 12 normal subjects. Serum concentrations were measured during a period of 8 h, and urine recovery was measured during 24 h. The significant parameters of bioavailability of an orally administered substance were determined. The maximal serum concentrations (y(max)) for cephalexin, cefaclor, cefadroxil, and CGP 9000 (in milligrams per liter) were: 38.8 +/- 8.1; 34.6 +/- 7.8; 33.0 +/- 5.4; and 23.3 +/- 7.3, respectively. The areas under the curve (in hours x milligrams per liter) were: 93.0 +/- 14.8; 74.5 +/- 9.9; 70.1 +/- 9.0; and 108.5 +/- 18.4, respectively. In a further crossover study with six subjects, 1,000 mg of cephalexin and of cefadroxil were given during a standard breakfast. The y(max) of cephalexin decreased to 23.1 +/- 6.6 mg/liter, in contrast to cefadroxil, with an unchanged y(max) of 32.7 +/- 3.4 mg/liter.", "contents": "Comparative pharmacokinetics of cephalexin, cefaclor, cefadroxil, and CGP 9000. In a randomized crossover study, the pharmacokinetics of three new cephalosporin antibiotics, cefaclor, cefadroxil, and CGP 9000, in comparison to cephalexin, were determined after oral administration, by capsules, of 1,000 mg on an empty stomach in 12 normal subjects. Serum concentrations were measured during a period of 8 h, and urine recovery was measured during 24 h. The significant parameters of bioavailability of an orally administered substance were determined. The maximal serum concentrations (y(max)) for cephalexin, cefaclor, cefadroxil, and CGP 9000 (in milligrams per liter) were: 38.8 +/- 8.1; 34.6 +/- 7.8; 33.0 +/- 5.4; and 23.3 +/- 7.3, respectively. The areas under the curve (in hours x milligrams per liter) were: 93.0 +/- 14.8; 74.5 +/- 9.9; 70.1 +/- 9.0; and 108.5 +/- 18.4, respectively. In a further crossover study with six subjects, 1,000 mg of cephalexin and of cefadroxil were given during a standard breakfast. The y(max) of cephalexin decreased to 23.1 +/- 6.6 mg/liter, in contrast to cefadroxil, with an unchanged y(max) of 32.7 +/- 3.4 mg/liter."}
{"id": "PMID:475367", "title": "Inhibition of cilia regeneration of Tetrahymena by ansamitocins, new antitumor antibiotics.", "content": "Ansamitocins inhibited cilia regeneration of partially deciliated Tetrahymena pyriformis W, and the activity depended on the acyl groups at the C3 position.", "contents": "Inhibition of cilia regeneration of Tetrahymena by ansamitocins, new antitumor antibiotics. Ansamitocins inhibited cilia regeneration of partially deciliated Tetrahymena pyriformis W, and the activity depended on the acyl groups at the C3 position."}
{"id": "PMID:475368", "title": "In vitro susceptibility of Ureaplasma urealyticum to rosaramicin.", "content": "Thirty-two clinical isolates of Ureaplasma urealyticum were inhibited by 0.036 mug of rosaramicin per ml, whereas 25 (78%) and 13 (41%) of the strains were inhibited by this concentration of minocycline and tetracycline, respectively. These results suggest that rosaramicin may be useful for the treatment of U. urealyticum infections.", "contents": "In vitro susceptibility of Ureaplasma urealyticum to rosaramicin. Thirty-two clinical isolates of Ureaplasma urealyticum were inhibited by 0.036 mug of rosaramicin per ml, whereas 25 (78%) and 13 (41%) of the strains were inhibited by this concentration of minocycline and tetracycline, respectively. These results suggest that rosaramicin may be useful for the treatment of U. urealyticum infections."}
{"id": "PMID:475369", "title": "In vitro of cefotaxime against cephalothin-resistant clinical isolates.", "content": "Cefotaxime is more active than six other cephalosporins against 150 cephalothin-resistant Enterobacteriaceae strains and is the only drug which is more active than ampicillin against Haemophilus. It shows a potentially useful activity against Pseudomonas.", "contents": "In vitro of cefotaxime against cephalothin-resistant clinical isolates. Cefotaxime is more active than six other cephalosporins against 150 cephalothin-resistant Enterobacteriaceae strains and is the only drug which is more active than ampicillin against Haemophilus. It shows a potentially useful activity against Pseudomonas."}
{"id": "PMID:475370", "title": "Comparative in vitro activity of five cephalosporins against Lactobacilli.", "content": "Of five parenteral cephalosporins tested against 43 lactobacilli, cephaloridine, cefazolin, and cefamandole were the most active inhibitory and bactericidal agents. Timed-kill analysis revealed a slow bactericidal effect, with significant declines in mean minimal bactericidal concentration values at 48 h versus 24 h.", "contents": "Comparative in vitro activity of five cephalosporins against Lactobacilli. Of five parenteral cephalosporins tested against 43 lactobacilli, cephaloridine, cefazolin, and cefamandole were the most active inhibitory and bactericidal agents. Timed-kill analysis revealed a slow bactericidal effect, with significant declines in mean minimal bactericidal concentration values at 48 h versus 24 h."}
{"id": "PMID:475371", "title": "Isolation and properties of metronidazole-resistant mutants of Bacteroides fragilis.", "content": "Metronidazole-resistant mutants of Bacteroides fragilis, isolated after mutagenesis, had diminished ability to take up and metabolize the drug. All the metronidazole-resistant strains had depressed levels of pyruvate dehydrogenase compared with parent cultures. Their end products of glucose metabolism also differed from normal B. fragilis products and were consistent with deficiencies in pyruvate dehydrogenase activity.", "contents": "Isolation and properties of metronidazole-resistant mutants of Bacteroides fragilis. Metronidazole-resistant mutants of Bacteroides fragilis, isolated after mutagenesis, had diminished ability to take up and metabolize the drug. All the metronidazole-resistant strains had depressed levels of pyruvate dehydrogenase compared with parent cultures. Their end products of glucose metabolism also differed from normal B. fragilis products and were consistent with deficiencies in pyruvate dehydrogenase activity."}
{"id": "PMID:475372", "title": "Susceptibility of Campylobacter fetus subsp. jejuni to twenty antimicrobiol agents.", "content": "One hundred recent clinical isolates of Campylobacter fetus subsp. jejuni were tested by an agar dilution technique for susceptibility to each of 20 antimicrobial agents. Doxycycline and gentamicin were the most active of the drugs examined, inhibiting all strains at concentrations achievable in serum. Although the median minimal inhibitory concentration of erythromycin was low, 8% of the isolates were highly resistant. All isolates of Campylobacter fetus subsp. jejuni were relatively resistant to the beta-lactam antibiotics. Some strains were highly resistant to metronidazole and tinidazole.", "contents": "Susceptibility of Campylobacter fetus subsp. jejuni to twenty antimicrobiol agents. One hundred recent clinical isolates of Campylobacter fetus subsp. jejuni were tested by an agar dilution technique for susceptibility to each of 20 antimicrobial agents. Doxycycline and gentamicin were the most active of the drugs examined, inhibiting all strains at concentrations achievable in serum. Although the median minimal inhibitory concentration of erythromycin was low, 8% of the isolates were highly resistant. All isolates of Campylobacter fetus subsp. jejuni were relatively resistant to the beta-lactam antibiotics. Some strains were highly resistant to metronidazole and tinidazole."}
{"id": "PMID:475373", "title": "Comparison of a micromethod for performance of the serum bactericidal test with the standard tube dilution method.", "content": "A micromethod for performance of the serum bactericidal test is described, and the results obtained with this method are compared with those obtained with the standard tube dilution macromethod. An agreement within +/- 1 dilution was achieved in 23 of 25 (92%) determinations of the serum bactericidal titer. The micromethod used approximately one-third of the amount of pooled normal human serum and of the technician's time required for performance of the macromethod. The micromethod offers an accurate and economical alternative to the macromethod for the performance of the serum bactericidal test and is particularly useful with infants and children since it minimizes blood loss.", "contents": "Comparison of a micromethod for performance of the serum bactericidal test with the standard tube dilution method. A micromethod for performance of the serum bactericidal test is described, and the results obtained with this method are compared with those obtained with the standard tube dilution macromethod. An agreement within +/- 1 dilution was achieved in 23 of 25 (92%) determinations of the serum bactericidal titer. The micromethod used approximately one-third of the amount of pooled normal human serum and of the technician's time required for performance of the macromethod. The micromethod offers an accurate and economical alternative to the macromethod for the performance of the serum bactericidal test and is particularly useful with infants and children since it minimizes blood loss."}
{"id": "PMID:475374", "title": "Human fibroblast interferon for clinical trials: production, partial purification, and characterization.", "content": "The production and partial purification of human fibroblast interferon for performing clinical trials is described. The interferon was produced by superinduction (exposure to riboinosinic-ribocytidylic acid, cycloheximide, and actinomycin D) of large numbers of human diploid fibroblast cultures. The yield averaged 750 units per cm(2) of culture area. The interferon was concentrated and purified by a two-step procedure involving acid desorption from controlled-pore glass beads and dialysis against polyethylene glycol. Human plasma protein was added as a stabilizer. The lyophilized end product had a specific activity of 0.5 x 10(6) to 1 x 10(6) units/mg of protein; it could be reconstituted for injection at a concentration of 2 x 10(6) units/ml. The composition of this interferon was characterized by crossed immunoelectrophoresis with polyspecific antibodies prepared against the principal sources of potential contaminants: human serum, calf serum, and normal human fibroblasts. Several components of each source were detected. Although the major component of calf serum, bovine serum albumin, was absent, other minor components were retained by the production and purification sequence. One of the main contaminants of fibroblast origin was found to be fibronectin.", "contents": "Human fibroblast interferon for clinical trials: production, partial purification, and characterization. The production and partial purification of human fibroblast interferon for performing clinical trials is described. The interferon was produced by superinduction (exposure to riboinosinic-ribocytidylic acid, cycloheximide, and actinomycin D) of large numbers of human diploid fibroblast cultures. The yield averaged 750 units per cm(2) of culture area. The interferon was concentrated and purified by a two-step procedure involving acid desorption from controlled-pore glass beads and dialysis against polyethylene glycol. Human plasma protein was added as a stabilizer. The lyophilized end product had a specific activity of 0.5 x 10(6) to 1 x 10(6) units/mg of protein; it could be reconstituted for injection at a concentration of 2 x 10(6) units/ml. The composition of this interferon was characterized by crossed immunoelectrophoresis with polyspecific antibodies prepared against the principal sources of potential contaminants: human serum, calf serum, and normal human fibroblasts. Several components of each source were detected. Although the major component of calf serum, bovine serum albumin, was absent, other minor components were retained by the production and purification sequence. One of the main contaminants of fibroblast origin was found to be fibronectin."}
{"id": "PMID:475375", "title": "Human fibroblast interferon for clinical trials: pharmacokinetics and tolerability in experimental animals and humans.", "content": "Human fibroblast interferon (F-interferon) purified by adsorption on controlled-pore glass was given intramuscularly to patients at daily dosages of up to 20 x 10(6) units. Serum levels of antiviral activity were low or undetectable. In contrast, reasonably high serum titers were found in patients receiving interferon prepared from leukocytes (L-interferon). Similarly, in rabbits lower serum titers were seen with F-interferon than with L-interferon. These results are at variance with those obtained earlier (V. G. Edy, A. Billiau, and P. De Somer, J. Infect. Dis. 133:A18-A21, 1976). Possible explanations for this discrepancy are discussed. The F-interferon evoked febrile reactions, delayed skin reactivity, and transitory lymphopenia in humans. Some patients developed an allergic state of the reaginic type as evidenced by a weal and flare reaction after intradermal challenge. However, these patients did not show allergic symptoms after intramuscular injections. None of the side effects was severe enough to prohibit continuation of the treatment; most of them seemed to be due to contaminants not removed by the purification method. The possibility is considered that some of the side effects, e.g., delayed skin reactivity, are sufficiently specific to justify identification of the active principals.", "contents": "Human fibroblast interferon for clinical trials: pharmacokinetics and tolerability in experimental animals and humans. Human fibroblast interferon (F-interferon) purified by adsorption on controlled-pore glass was given intramuscularly to patients at daily dosages of up to 20 x 10(6) units. Serum levels of antiviral activity were low or undetectable. In contrast, reasonably high serum titers were found in patients receiving interferon prepared from leukocytes (L-interferon). Similarly, in rabbits lower serum titers were seen with F-interferon than with L-interferon. These results are at variance with those obtained earlier (V. G. Edy, A. Billiau, and P. De Somer, J. Infect. Dis. 133:A18-A21, 1976). Possible explanations for this discrepancy are discussed. The F-interferon evoked febrile reactions, delayed skin reactivity, and transitory lymphopenia in humans. Some patients developed an allergic state of the reaginic type as evidenced by a weal and flare reaction after intradermal challenge. However, these patients did not show allergic symptoms after intramuscular injections. None of the side effects was severe enough to prohibit continuation of the treatment; most of them seemed to be due to contaminants not removed by the purification method. The possibility is considered that some of the side effects, e.g., delayed skin reactivity, are sufficiently specific to justify identification of the active principals."}
{"id": "PMID:475376", "title": "Ventricular fluid levels of chloramphenicol in infants.", "content": "Investigation of two infants suggested that peak ventricular fluid levels of chloramphenicol after intravenous administration were achieved after 3 h. The penetration of chloramphenicol into ventricular fluid may be unpredictable. In one patient, the peak ventricular fluid level was 57.5% of the peak serum level, and it was only 22.5% in the other patient. This observation may explain some of the past treatment failures when chloramphenicol was utilized in patients with gram-negative ventriculitis and meningitis.", "contents": "Ventricular fluid levels of chloramphenicol in infants. Investigation of two infants suggested that peak ventricular fluid levels of chloramphenicol after intravenous administration were achieved after 3 h. The penetration of chloramphenicol into ventricular fluid may be unpredictable. In one patient, the peak ventricular fluid level was 57.5% of the peak serum level, and it was only 22.5% in the other patient. This observation may explain some of the past treatment failures when chloramphenicol was utilized in patients with gram-negative ventriculitis and meningitis."}
{"id": "PMID:475377", "title": "Pharmacokinetics of cephradine suspension infants and children.", "content": "The pharmacokinetics of cephradine suspension were studied in 16 infants and children who were 13 months to 8 years and 3 months of age (means age, 3.5 years). Mean peak concentrations of 21.3 and 9.9 mug/ml were achieved at 30 min after administration of 60-mg/kg doses to fasting and nonfasting patients. The area under the serum concentration-time curve was 26% larger in fasting than in fed subjects. The half-life of cephradine in serum was 0.8 and 1.0 h in fasting and fed groups, respectively. Antimicrobial activity was detected in 49% of all salivary samples; in 75% of specimens, the concentrations were less than the 50% minimum inhibitory concentration for most pneumococci and group A streptococci. Urinary concentrations of cephradine ranged from 28 to 8,760 mug/ml and were independent of feeding status.", "contents": "Pharmacokinetics of cephradine suspension infants and children. The pharmacokinetics of cephradine suspension were studied in 16 infants and children who were 13 months to 8 years and 3 months of age (means age, 3.5 years). Mean peak concentrations of 21.3 and 9.9 mug/ml were achieved at 30 min after administration of 60-mg/kg doses to fasting and nonfasting patients. The area under the serum concentration-time curve was 26% larger in fasting than in fed subjects. The half-life of cephradine in serum was 0.8 and 1.0 h in fasting and fed groups, respectively. Antimicrobial activity was detected in 49% of all salivary samples; in 75% of specimens, the concentrations were less than the 50% minimum inhibitory concentration for most pneumococci and group A streptococci. Urinary concentrations of cephradine ranged from 28 to 8,760 mug/ml and were independent of feeding status."}
{"id": "PMID:475378", "title": "Effects of dextranases on attachment of Streptococcus mutans to hydroxyapatite.", "content": "A Fusarium dextranase and a Penicillium dextranase were compared for their relative ability to quantitatively reduce the adsorption of (3)H-labeled Steptococcus mutans cells onto hydroxyapatite. Fusarium dextranase-treated hydroxyapatite disks caused a statistically significant decrease in the hydroxyapatite adsorption of both the OMZ 176 and NCTC 10449 strains of S. mutans relative to untreated control disks. The extent of initial bacterial adsorption was not promoted by sucrose-dependent glucan synthesis. Since the Fusarium dextranase has a much greater affinity for hydroxyapatite than the Penicillium dextranase, it could represent an enzyme with improved decay-preventive therapeutic properties. This was concluded because the Fusarium dextranase may interfere with both the initial attachment and later glucan-dependent accumulation of dental plaque microorganisms.", "contents": "Effects of dextranases on attachment of Streptococcus mutans to hydroxyapatite. A Fusarium dextranase and a Penicillium dextranase were compared for their relative ability to quantitatively reduce the adsorption of (3)H-labeled Steptococcus mutans cells onto hydroxyapatite. Fusarium dextranase-treated hydroxyapatite disks caused a statistically significant decrease in the hydroxyapatite adsorption of both the OMZ 176 and NCTC 10449 strains of S. mutans relative to untreated control disks. The extent of initial bacterial adsorption was not promoted by sucrose-dependent glucan synthesis. Since the Fusarium dextranase has a much greater affinity for hydroxyapatite than the Penicillium dextranase, it could represent an enzyme with improved decay-preventive therapeutic properties. This was concluded because the Fusarium dextranase may interfere with both the initial attachment and later glucan-dependent accumulation of dental plaque microorganisms."}
{"id": "PMID:475403", "title": "Thoracic duct cyst in the anterior mediastinum.", "content": "Case report of a 60-year old asymptomatic male with a chylous cyst in the right anterior mediastinum. It communicated with the thoracic duct through a narrow vessel with a length of 4 cm. During the eight years of pre-operative observation the cyst varied in size. At one time it disappeared completely, though temporarily. The rarity of this type of mediastinal tumor is stressed.", "contents": "Thoracic duct cyst in the anterior mediastinum. Case report of a 60-year old asymptomatic male with a chylous cyst in the right anterior mediastinum. It communicated with the thoracic duct through a narrow vessel with a length of 4 cm. During the eight years of pre-operative observation the cyst varied in size. At one time it disappeared completely, though temporarily. The rarity of this type of mediastinal tumor is stressed."}
{"id": "PMID:475404", "title": "Chylous ascites after resection of an abdominal aortic aneurysm.", "content": "A case of chylous ascites after resection of an abdominal aneurysm in a 72-year old male is presented. The complication is extremely rare. Therapy should start medically with a diuretic and medium chain triglyceride (M.C.T) diet. This is the ninth reported case of iatrogenic chylous ascites and the second after resection of an abdominal aortic aneurysm.", "contents": "Chylous ascites after resection of an abdominal aortic aneurysm. A case of chylous ascites after resection of an abdominal aneurysm in a 72-year old male is presented. The complication is extremely rare. Therapy should start medically with a diuretic and medium chain triglyceride (M.C.T) diet. This is the ninth reported case of iatrogenic chylous ascites and the second after resection of an abdominal aortic aneurysm."}
{"id": "PMID:475405", "title": "Cross clamping the lower abdominal aorta during vascular surgery.", "content": "The effect of cross clamping the lower abdominal aorta on systemic blood pressure and right and left heart pressure was studied in 17 patients requiring surgical treatment for abdominal aortic aneurysm or aortic occlusive disease, in neuroleptanaesthesia. After cross clamping the systemic systolic blood pressure rose 24 mmHg (p is less than 0.01) and the systemic diastolic blood pressure 8 mmHg (p is less than 0.01). There was no influence of cross clamping on the right (CVP) and and left (PCWP) heart filling pressure even in patients with preexistent readings beyond the normal range.", "contents": "Cross clamping the lower abdominal aorta during vascular surgery. The effect of cross clamping the lower abdominal aorta on systemic blood pressure and right and left heart pressure was studied in 17 patients requiring surgical treatment for abdominal aortic aneurysm or aortic occlusive disease, in neuroleptanaesthesia. After cross clamping the systemic systolic blood pressure rose 24 mmHg (p is less than 0.01) and the systemic diastolic blood pressure 8 mmHg (p is less than 0.01). There was no influence of cross clamping on the right (CVP) and and left (PCWP) heart filling pressure even in patients with preexistent readings beyond the normal range."}
{"id": "PMID:475406", "title": "Bladder fistulae in Crohn's disease.", "content": "Ten patients with various stages of enterovesical fistulization in Crohn's disease are discussed. These fistulae can develop at different times in the course of the disease, but may also cause the presenting symptoms. Cystoscopy and radiologic examination rarely visualizes the fistulae. Conservative treatment by medication can sometimes suppress the symptoms, but cannot cure. Surgical treatment, which can consist of resection of intestine but need not involve resection of parts of the bladder, gives good results at least so far as the urinary infection is concerned.", "contents": "Bladder fistulae in Crohn's disease. Ten patients with various stages of enterovesical fistulization in Crohn's disease are discussed. These fistulae can develop at different times in the course of the disease, but may also cause the presenting symptoms. Cystoscopy and radiologic examination rarely visualizes the fistulae. Conservative treatment by medication can sometimes suppress the symptoms, but cannot cure. Surgical treatment, which can consist of resection of intestine but need not involve resection of parts of the bladder, gives good results at least so far as the urinary infection is concerned."}
{"id": "PMID:475408", "title": "Neonatal form of dystrophia myotonica. Five cases in preterm babies and a review of earlier reports.", "content": "Five preterm babies with the neonatal form of dystrophia myotonica are reported. In addition to the generally accepted signs and symptoms of the disease, two other features were present in these patients; oedema was notable in all 5 babies and 4 had unexplained haematomas. It is suggested that premature birth may be a result of severe involvement and that prematurity further aggravates the symptoms.", "contents": "Neonatal form of dystrophia myotonica. Five cases in preterm babies and a review of earlier reports. Five preterm babies with the neonatal form of dystrophia myotonica are reported. In addition to the generally accepted signs and symptoms of the disease, two other features were present in these patients; oedema was notable in all 5 babies and 4 had unexplained haematomas. It is suggested that premature birth may be a result of severe involvement and that prematurity further aggravates the symptoms."}
{"id": "PMID:475409", "title": "Plasma 17OH-progesterone concentrations in newborn infants.", "content": "Plasma concentrations of 17OH-progesterone were determined in 60 normal newborn infants aged between 3 and 36 hours. Mean levels decreased rapidly during this time after removal of the placental contribution of this steroid. A further 70 normal infants, studied between ages 2 and 7 days, showed a mean plasma 17OH-progesterone concentration of 3.5 nmol/1 (1.2 ng/ml). By comparison, plasma concentrations in untreated infants with congenital adrenal hyperplasia were markedly raised. At 36 hours of age, there was an obvious difference between plasma levels of this steroid in normal and affected infants. Determination of plasma 17OH-progesterone concentrations are valuable in the evaluation of disorders of sexual differentiation and electrolyte balance in newborn infants, provided due care is given to the timing of sample collections.", "contents": "Plasma 17OH-progesterone concentrations in newborn infants. Plasma concentrations of 17OH-progesterone were determined in 60 normal newborn infants aged between 3 and 36 hours. Mean levels decreased rapidly during this time after removal of the placental contribution of this steroid. A further 70 normal infants, studied between ages 2 and 7 days, showed a mean plasma 17OH-progesterone concentration of 3.5 nmol/1 (1.2 ng/ml). By comparison, plasma concentrations in untreated infants with congenital adrenal hyperplasia were markedly raised. At 36 hours of age, there was an obvious difference between plasma levels of this steroid in normal and affected infants. Determination of plasma 17OH-progesterone concentrations are valuable in the evaluation of disorders of sexual differentiation and electrolyte balance in newborn infants, provided due care is given to the timing of sample collections."}
{"id": "PMID:475410", "title": "Continuing need for mineralocorticoid therapy in salt-losing congenital adrenal hyperplasia.", "content": "Four patients with salt-losing congenital adrenal hyperplasia (CAH) who had stopped mineralocorticoid therapy for several years, showed raised plasma concentrations of 17OH-progesterone and plasma renin activity, despite adequate glucoticoid therapy. One patient was able to reduce urinary sodium excretion when the sodium intake was restricted. Another patient who was a salt-loser, developed signs of an adrenal crisis when salt deprived. In comparison, one nonsalt-loser and 2 normal subjects decreased urinary sodium excretion in response to sodium restriction. The addition of fludrocortisone (100 micrograms) to usual maintenance doses of glucocorticoid, resulted in normal levels of plasma 17OH-progesterone and plasma renin activity in all 4 salt-losers. Two female salt-losers, with raised plasma testosterone concentrations, began menstruating when their plasma testosterone concentrations returned to normal after treatment with fludrocortisone. It is recommended that salt-losing CAH patients should be given mineralocorticoid, in addition to glucocorticoid therapy, at least until adult life.", "contents": "Continuing need for mineralocorticoid therapy in salt-losing congenital adrenal hyperplasia. Four patients with salt-losing congenital adrenal hyperplasia (CAH) who had stopped mineralocorticoid therapy for several years, showed raised plasma concentrations of 17OH-progesterone and plasma renin activity, despite adequate glucoticoid therapy. One patient was able to reduce urinary sodium excretion when the sodium intake was restricted. Another patient who was a salt-loser, developed signs of an adrenal crisis when salt deprived. In comparison, one nonsalt-loser and 2 normal subjects decreased urinary sodium excretion in response to sodium restriction. The addition of fludrocortisone (100 micrograms) to usual maintenance doses of glucocorticoid, resulted in normal levels of plasma 17OH-progesterone and plasma renin activity in all 4 salt-losers. Two female salt-losers, with raised plasma testosterone concentrations, began menstruating when their plasma testosterone concentrations returned to normal after treatment with fludrocortisone. It is recommended that salt-losing CAH patients should be given mineralocorticoid, in addition to glucocorticoid therapy, at least until adult life."}
{"id": "PMID:475411", "title": "Creatine phosphokinase levels in the newborn and their use in screening for Duchenne muscular dystrophy.", "content": "The PKU sample, if taken after the 3rd day, is suitable for Duchenne muscular dystrophy screening using creatine phosphokinase (CPK) levels. 101 babies (53 girls and 48 boys) had CPK levels measured on filter paper blood specimens taken by heel prick on the 1st and 4th days of life. The method used was that described by Zellweger and Antonik (1975). The CPK levels were markedly raised on the 1st day of life but by the 4th they had reduced to within 3 times the upper limit of the normal range for adults. 10,000 boys were then screened for Duchenne muscular dystrophy using the PKU sample, and 2 cases were found.", "contents": "Creatine phosphokinase levels in the newborn and their use in screening for Duchenne muscular dystrophy. The PKU sample, if taken after the 3rd day, is suitable for Duchenne muscular dystrophy screening using creatine phosphokinase (CPK) levels. 101 babies (53 girls and 48 boys) had CPK levels measured on filter paper blood specimens taken by heel prick on the 1st and 4th days of life. The method used was that described by Zellweger and Antonik (1975). The CPK levels were markedly raised on the 1st day of life but by the 4th they had reduced to within 3 times the upper limit of the normal range for adults. 10,000 boys were then screened for Duchenne muscular dystrophy using the PKU sample, and 2 cases were found."}
{"id": "PMID:475412", "title": "Serum 25-hydroxy-vitamin D in hepatobiliary disease in infancy.", "content": "Serum 25-hydroxy-vitamin D (25-OHD) concentrations were measured in 49 patients with hepatobiliary disease in infancy. Low mean values were found in groups of patients with biliary atresia, neonatal hepatitis, choledochal cyst, and chronic intrahepatic cholestatic syndrome. In the group of patients with surgically repaired biliary atresia, the mean value did not differ from normal. Parenteral vitamin D increased 25-OHD in serum in patients with biliary atresia, but did not do so in one patient with neonatal hepatitis. In contrast, oral vitamin D did not increase serum 25-OHD concentrations in patients with biliary atresia. It is concluded that the reduction of serum 25-OHD seen in biliary atresia was largely due to the malabsorption of vitamin D, while in neonatal hepatitis it was due to impairment of 25-hydroxylation of the vitamin.", "contents": "Serum 25-hydroxy-vitamin D in hepatobiliary disease in infancy. Serum 25-hydroxy-vitamin D (25-OHD) concentrations were measured in 49 patients with hepatobiliary disease in infancy. Low mean values were found in groups of patients with biliary atresia, neonatal hepatitis, choledochal cyst, and chronic intrahepatic cholestatic syndrome. In the group of patients with surgically repaired biliary atresia, the mean value did not differ from normal. Parenteral vitamin D increased 25-OHD in serum in patients with biliary atresia, but did not do so in one patient with neonatal hepatitis. In contrast, oral vitamin D did not increase serum 25-OHD concentrations in patients with biliary atresia. It is concluded that the reduction of serum 25-OHD seen in biliary atresia was largely due to the malabsorption of vitamin D, while in neonatal hepatitis it was due to impairment of 25-hydroxylation of the vitamin."}
{"id": "PMID:475413", "title": "Relationship between urinary and blood glucose in diabetic children.", "content": "The relationship of urine glucose concentration with 'average' blood glucose concentration for the corresponding period was investigated in 10 juvenile diabetics during the performance of 15 inpatient metabolic profiles. 0% glucosuria was associated with 'average' blood glucose concentrations less than 6.4 mmol/l (less than 115 mg/100ml), 2% glycosuria with concentrations greater than 8.6 mmol/l (greater than 154 mg/100ml), and over 5% glycosuria with concentrations greater than 11.3 mmol/l (greater than 204 mg/100ml). Urinary glucose loss was large (greater than 278 mmol; greater than 5g) when the 'average' blood glucose was greater than 11.1 mmol/l (greater than 200 mg/100ml). Because many diabetics have blood glucose concentrations up to 11.1 mmol/l, it is advantageous if glucosuria up to 5% is detectable by routine home urine tests. The 2-drop Clinitest method detects glycosuria up to 5% without significant loss of accuracy and is recommended in preference to the 5-drop method.", "contents": "Relationship between urinary and blood glucose in diabetic children. The relationship of urine glucose concentration with 'average' blood glucose concentration for the corresponding period was investigated in 10 juvenile diabetics during the performance of 15 inpatient metabolic profiles. 0% glucosuria was associated with 'average' blood glucose concentrations less than 6.4 mmol/l (less than 115 mg/100ml), 2% glycosuria with concentrations greater than 8.6 mmol/l (greater than 154 mg/100ml), and over 5% glycosuria with concentrations greater than 11.3 mmol/l (greater than 204 mg/100ml). Urinary glucose loss was large (greater than 278 mmol; greater than 5g) when the 'average' blood glucose was greater than 11.1 mmol/l (greater than 200 mg/100ml). Because many diabetics have blood glucose concentrations up to 11.1 mmol/l, it is advantageous if glucosuria up to 5% is detectable by routine home urine tests. The 2-drop Clinitest method detects glycosuria up to 5% without significant loss of accuracy and is recommended in preference to the 5-drop method."}
{"id": "PMID:475414", "title": "Absorption of chlorhexidine from the intact skin of newborn infants.", "content": "34 newborn infants who had been bathed in a standard manner with Hibiscrub were studied to find out whether it was absorbed percutaneously. Low levels of chlorhexidine were found in the blood of all 10 babies sampled by heel prick, and 5 of 24 from whom venous blood was taken. The detection of chlorhexidine varied greatly with the method and timing of sampling, and no correlation was found between gestational or postnatal age and chlorhexidine levels.", "contents": "Absorption of chlorhexidine from the intact skin of newborn infants. 34 newborn infants who had been bathed in a standard manner with Hibiscrub were studied to find out whether it was absorbed percutaneously. Low levels of chlorhexidine were found in the blood of all 10 babies sampled by heel prick, and 5 of 24 from whom venous blood was taken. The detection of chlorhexidine varied greatly with the method and timing of sampling, and no correlation was found between gestational or postnatal age and chlorhexidine levels."}
{"id": "PMID:475415", "title": "Infective pericarditis in Nigerian children.", "content": "53 children with infective pericarditis were seen at the University College Hospital, Ibadan, between 1967 and 1976. Their ages ranged from 10 days to 15 years but 53% of them were aged 5 years and below. Cough, fever, and breathlessness were the most common symptoms; cardiac decompensation was evident in over 30% of them, 23% had muffled heart sounds, but a pericardial friction rub was audible in only one. The main pathogens identified were Mycobacterium tuberculosis (11 cases), Staphylococcus aureus (11 cases), Escherichia coli (4 cases), Pneumococcus and Pseudomonas (3 cases each). Most of the patients had some other associated infection--such as, bronchopneumonia (12 cases), empyema thoracis (10 cases), lung abscess (10 cases), septicaemis (6 cases), and osteomyelitis (3 cases). Errors in diagnosis were common, the diagnosis having been missed in 72% of the cases identified at necropsy. Even if the correct diagnosis had been made during life and appropriate treatment given, the mortality rate (36%) was high. It is suggested that the onset of cardiac failure in any child with bronchopneumonia, empyema, or lung abscess should always arouse a suspicion of infective pericarditis.", "contents": "Infective pericarditis in Nigerian children. 53 children with infective pericarditis were seen at the University College Hospital, Ibadan, between 1967 and 1976. Their ages ranged from 10 days to 15 years but 53% of them were aged 5 years and below. Cough, fever, and breathlessness were the most common symptoms; cardiac decompensation was evident in over 30% of them, 23% had muffled heart sounds, but a pericardial friction rub was audible in only one. The main pathogens identified were Mycobacterium tuberculosis (11 cases), Staphylococcus aureus (11 cases), Escherichia coli (4 cases), Pneumococcus and Pseudomonas (3 cases each). Most of the patients had some other associated infection--such as, bronchopneumonia (12 cases), empyema thoracis (10 cases), lung abscess (10 cases), septicaemis (6 cases), and osteomyelitis (3 cases). Errors in diagnosis were common, the diagnosis having been missed in 72% of the cases identified at necropsy. Even if the correct diagnosis had been made during life and appropriate treatment given, the mortality rate (36%) was high. It is suggested that the onset of cardiac failure in any child with bronchopneumonia, empyema, or lung abscess should always arouse a suspicion of infective pericarditis."}
{"id": "PMID:475416", "title": "Adder bites in children.", "content": "16 children with adder bites were admitted to hospital in Southampton in the years 1969--77. Two children were severely poisoned; these 2, and a 3rd, fulfilled suggested criteria for antivenom administration but they recovered without it. Three children had no treatment, and 9 children no analgesia. Prolonged morbidity was not seen in these children. Careful observation in hospital was the most important factor in management, with early clinical improvement obviating the need for antivenom.", "contents": "Adder bites in children. 16 children with adder bites were admitted to hospital in Southampton in the years 1969--77. Two children were severely poisoned; these 2, and a 3rd, fulfilled suggested criteria for antivenom administration but they recovered without it. Three children had no treatment, and 9 children no analgesia. Prolonged morbidity was not seen in these children. Careful observation in hospital was the most important factor in management, with early clinical improvement obviating the need for antivenom."}
{"id": "PMID:475417", "title": "Pathogenesis of oxytocin-induced neonatal hyperbilirubinaemia.", "content": "100 term (gestation at least 37 weeks), vertex presenting, vaginally delivered, and fetomaternal blood-group-compatible neonates were studied to evaluate the pathogenesis of neonatal hyperbilirubinaemia induced by oxytocin. 50 infants were born after oxytocin infusion for augmentation of labour and the other 50 were delivered spontaneously. The babies born after oxytocin induction of labour attained significantly higher serum bilirubin levels at age 72 +/- 12 hours than the controls. Infants born after oxytocin showed significant hyponatraemia, hypo-osmolality, and enhanced osmotic fragility of erythrocytes at birth. These biochemical and physiological alterations can be explained by the antidiuretic effects of oxytocin and concomitant administration of large quantities of electrolyte-free dextrose solutions used to administer it. Our observations suggest that cord serum sodium and/or osmolality should be estimated and infants with serum sodium less than 125 mmol/l and/or osmolality less than 260 mmol/kg should be considered for prophylactic administration of phenobarbitone.", "contents": "Pathogenesis of oxytocin-induced neonatal hyperbilirubinaemia. 100 term (gestation at least 37 weeks), vertex presenting, vaginally delivered, and fetomaternal blood-group-compatible neonates were studied to evaluate the pathogenesis of neonatal hyperbilirubinaemia induced by oxytocin. 50 infants were born after oxytocin infusion for augmentation of labour and the other 50 were delivered spontaneously. The babies born after oxytocin induction of labour attained significantly higher serum bilirubin levels at age 72 +/- 12 hours than the controls. Infants born after oxytocin showed significant hyponatraemia, hypo-osmolality, and enhanced osmotic fragility of erythrocytes at birth. These biochemical and physiological alterations can be explained by the antidiuretic effects of oxytocin and concomitant administration of large quantities of electrolyte-free dextrose solutions used to administer it. Our observations suggest that cord serum sodium and/or osmolality should be estimated and infants with serum sodium less than 125 mmol/l and/or osmolality less than 260 mmol/kg should be considered for prophylactic administration of phenobarbitone."}
{"id": "PMID:475421", "title": "Clinical events relating to intraventricular haemorrhage in the newborn.", "content": "Continuous measurements of arterial pressures, heart rates, respiratory movements, and respiratory rates were made from birth in 44 infants at risk from intraventricular haemorrhage (IVH). 17 babies died with IVH, in 10 of whom the event was timed objectively. Events in these babies were compared with survivors of similar birthweights, gestational ages, severity of birth asphyxia, and severity of hyaline membrane disease (HMD). IVH followed severe HMD and was associated with cessation of the babies' own respiratory efforts while on a ventilator and also with characteristic cardiorespiratory events. The minimum arterial pressure before IVH was lower than in comparable babies who survived. It is suggested that fluctuations of systemic blood pressure from initial low levels may be important in the pathogenesis of IVH. It is possible that changes in cerebral blood flow are of even greater significance.", "contents": "Clinical events relating to intraventricular haemorrhage in the newborn. Continuous measurements of arterial pressures, heart rates, respiratory movements, and respiratory rates were made from birth in 44 infants at risk from intraventricular haemorrhage (IVH). 17 babies died with IVH, in 10 of whom the event was timed objectively. Events in these babies were compared with survivors of similar birthweights, gestational ages, severity of birth asphyxia, and severity of hyaline membrane disease (HMD). IVH followed severe HMD and was associated with cessation of the babies' own respiratory efforts while on a ventilator and also with characteristic cardiorespiratory events. The minimum arterial pressure before IVH was lower than in comparable babies who survived. It is suggested that fluctuations of systemic blood pressure from initial low levels may be important in the pathogenesis of IVH. It is possible that changes in cerebral blood flow are of even greater significance."}
{"id": "PMID:475422", "title": "Effect of feeding on neonatal oxygen consumption.", "content": "Oxygen consumption was measured in 9 short-gestation infants before feeding and for an hour after, using a closed-circuit metabolism chamber. Using the same system, O2 consumption of 9 term infants was measured for varying periods, beginning one hour from the end of the last feed. In the short-gestation infants a rise was found in the 15--45 minutes after feeding; O2 consumption then fell, and after 60 minutes had reached prefeed levels. The term infants showed no decrease with time after the first hour, as would be expected if the effect of feeding on O2 consumption extended beyond this. It is concluded that increased O2 demand after appropriate feeding does not extend beyond one hour postprandially.", "contents": "Effect of feeding on neonatal oxygen consumption. Oxygen consumption was measured in 9 short-gestation infants before feeding and for an hour after, using a closed-circuit metabolism chamber. Using the same system, O2 consumption of 9 term infants was measured for varying periods, beginning one hour from the end of the last feed. In the short-gestation infants a rise was found in the 15--45 minutes after feeding; O2 consumption then fell, and after 60 minutes had reached prefeed levels. The term infants showed no decrease with time after the first hour, as would be expected if the effect of feeding on O2 consumption extended beyond this. It is concluded that increased O2 demand after appropriate feeding does not extend beyond one hour postprandially."}
{"id": "PMID:475423", "title": "Hearing loss in very low birthweight infants treated with neonatal intensive care.", "content": "The hearing of 111 perinatal intensive care survivors of birthweights 1500 g or less was assessed at a mean age of 6 1/2 years (range 4--12). These 111 infants included 86% of the long-term survivors of this birthweight cared for in the newborn unit of University College Hospital, London, during the years 1966--72. All these infants were nursed in commercially available incubators for periods ranging from 2 to 80 days (mean 37) in which the mean noise threshold was 65 dB. Ten (9%) had sensory neural nearing losses, one (1%) infant had a congenital conductive hearing loss, and 21 (19%) infants had exudative otitis media with a mean loss of 25 dB. Apnoeic attacks in the neotal period were the most significant predictors of hearing loss in these infants (P less than 0.05) and an indirect serum filirubin level of at least 170 micromol/l (10 mg/100 ml) in the neonatal period had an additive effect (P less than 0.05). There was no evidence that ambient noise had affected the hearing of these very low birthweight infants.", "contents": "Hearing loss in very low birthweight infants treated with neonatal intensive care. The hearing of 111 perinatal intensive care survivors of birthweights 1500 g or less was assessed at a mean age of 6 1/2 years (range 4--12). These 111 infants included 86% of the long-term survivors of this birthweight cared for in the newborn unit of University College Hospital, London, during the years 1966--72. All these infants were nursed in commercially available incubators for periods ranging from 2 to 80 days (mean 37) in which the mean noise threshold was 65 dB. Ten (9%) had sensory neural nearing losses, one (1%) infant had a congenital conductive hearing loss, and 21 (19%) infants had exudative otitis media with a mean loss of 25 dB. Apnoeic attacks in the neotal period were the most significant predictors of hearing loss in these infants (P less than 0.05) and an indirect serum filirubin level of at least 170 micromol/l (10 mg/100 ml) in the neonatal period had an additive effect (P less than 0.05). There was no evidence that ambient noise had affected the hearing of these very low birthweight infants."}
{"id": "PMID:475424", "title": "1028 neonatal electrocardiograms.", "content": "Electrocardiograms were recorded from 1028 (99.4%) of 1034 consecutively born babies on the 1st or 2nd day of life. Abnormalities of cardiac rhythm or conduction were found in 49 (4.8%) babies. 17 babies had single or multiple premature beats during the recordings, and 7 babies showed sudden increases in R-R interval possibly due to sinoatrial node dysfunction. One baby had sinus or junctional bradycardia of less than 80 beats/min. One baby had an incessant reciprocating tachycardia and subsequently required digoxin for heart failure. Continuous 24-hour ECG monitoring in 25 babies with abnormalities of the screening ECG, and 25 babies without such abnormalities, failed to show any additional tachyarrhythmias or bradyarrhythmias warranting treatment. In view of the uncertain clinical significance of many neonatal arrhythmias and cardiac conduction disorders, more information concerning their natural history and relationship to sudden unexpected death in infancy is needed before recommending that neonatal ECG screening be generally adopted.", "contents": "1028 neonatal electrocardiograms. Electrocardiograms were recorded from 1028 (99.4%) of 1034 consecutively born babies on the 1st or 2nd day of life. Abnormalities of cardiac rhythm or conduction were found in 49 (4.8%) babies. 17 babies had single or multiple premature beats during the recordings, and 7 babies showed sudden increases in R-R interval possibly due to sinoatrial node dysfunction. One baby had sinus or junctional bradycardia of less than 80 beats/min. One baby had an incessant reciprocating tachycardia and subsequently required digoxin for heart failure. Continuous 24-hour ECG monitoring in 25 babies with abnormalities of the screening ECG, and 25 babies without such abnormalities, failed to show any additional tachyarrhythmias or bradyarrhythmias warranting treatment. In view of the uncertain clinical significance of many neonatal arrhythmias and cardiac conduction disorders, more information concerning their natural history and relationship to sudden unexpected death in infancy is needed before recommending that neonatal ECG screening be generally adopted."}
{"id": "PMID:475425", "title": "Sinusoidal heart rate rhythms in severe neonatal hypoxia.", "content": "Sinusoidal heart rate traces were detected in 8 patients, 6 of whom died; the 2 survivors showed evidence of gross cerebral damage. It is felt that the origin of the sinusoidal curve is probably central and reflects loss of central control of heart rate.", "contents": "Sinusoidal heart rate rhythms in severe neonatal hypoxia. Sinusoidal heart rate traces were detected in 8 patients, 6 of whom died; the 2 survivors showed evidence of gross cerebral damage. It is felt that the origin of the sinusoidal curve is probably central and reflects loss of central control of heart rate."}
{"id": "PMID:475426", "title": "Breath hydrogen test for detecting lactose malabsorption in infants and children. Prevalence of lactose malabsorption in Japanese children and adults.", "content": "The breath hydrogen test (BHT) was adapted for use in young infants and children. The diagnostic criterion of sugar malabsorption in the BHT was determined by oral administration of 0.5 g/kg of unabsorbable sugar (lactulose) to 21 healthy infants and children. A maximum increase in breath hydrogen less than 0.05 ml/min per m2 was observed in all subjects. A good correlation between results by the BHT and by the ordinary lactose tolerance test was obtained after oral administration of 2 g/kg lactose to 21 healthy infants and children, 2 congenital lactase-deficient infants, and 7 adults. Using this test, 80 healthy Japanese infants and children (aged between one month and 15 years) and 18 adults were examined for lactose malabsorption after a dose of 1 g/kg lactose. All infants and children under 2-years old absorbed lactose completely. The incidence of lactose malabsorption was 30% in 3-year, 36% in 4-year, 58% in 5-year, and 86% in 6-year-old children, 85% in schoolchildren, and 89% in adults. Thus the incidence of lactase deficiency gradually increases with age from 3 years, and about 90% of all normal Japanese adults are lactase-deficient.", "contents": "Breath hydrogen test for detecting lactose malabsorption in infants and children. Prevalence of lactose malabsorption in Japanese children and adults. The breath hydrogen test (BHT) was adapted for use in young infants and children. The diagnostic criterion of sugar malabsorption in the BHT was determined by oral administration of 0.5 g/kg of unabsorbable sugar (lactulose) to 21 healthy infants and children. A maximum increase in breath hydrogen less than 0.05 ml/min per m2 was observed in all subjects. A good correlation between results by the BHT and by the ordinary lactose tolerance test was obtained after oral administration of 2 g/kg lactose to 21 healthy infants and children, 2 congenital lactase-deficient infants, and 7 adults. Using this test, 80 healthy Japanese infants and children (aged between one month and 15 years) and 18 adults were examined for lactose malabsorption after a dose of 1 g/kg lactose. All infants and children under 2-years old absorbed lactose completely. The incidence of lactose malabsorption was 30% in 3-year, 36% in 4-year, 58% in 5-year, and 86% in 6-year-old children, 85% in schoolchildren, and 89% in adults. Thus the incidence of lactase deficiency gradually increases with age from 3 years, and about 90% of all normal Japanese adults are lactase-deficient."}
{"id": "PMID:475427", "title": "Defective Candida killing in childhood malnutrition.", "content": "This study shows that malnourished children have impaired candidacidal activity of leucocytes, a finding which runs parallel to the higher rates of isolation of Candida sp. from throat secretions. In well nournished subjects the mean candidacidal ability was 44.5% in the moderately undernourished group it was 17.6%, and in the severely malnourished group it was 13.7%. This impaired candidacidal capacity may be important in the establishment of large numbers of Candida sp. which are commonly found in the upper intestine and in the pathogenesis of diarrhoea in children with malnutrition.", "contents": "Defective Candida killing in childhood malnutrition. This study shows that malnourished children have impaired candidacidal activity of leucocytes, a finding which runs parallel to the higher rates of isolation of Candida sp. from throat secretions. In well nournished subjects the mean candidacidal ability was 44.5% in the moderately undernourished group it was 17.6%, and in the severely malnourished group it was 13.7%. This impaired candidacidal capacity may be important in the establishment of large numbers of Candida sp. which are commonly found in the upper intestine and in the pathogenesis of diarrhoea in children with malnutrition."}
{"id": "PMID:475428", "title": "Bronchial asthma in Nigerian children.", "content": "Clinical and laboratory studies on bronchial asthma in 200 Nigerian children who were seen during a 2 1/2-year period in Ibadan are described. Contrary to reports that the condition is rare in African children, after pulmonary tuberculosis, asthma is the next most common chronic chest disease in Ibadan. While many features of the disease are similar to those seen elsewhere, skin tests indicate that ascaris is the most common antigen associated with asthma, followed by Dermatophagoides pteronyssinus.", "contents": "Bronchial asthma in Nigerian children. Clinical and laboratory studies on bronchial asthma in 200 Nigerian children who were seen during a 2 1/2-year period in Ibadan are described. Contrary to reports that the condition is rare in African children, after pulmonary tuberculosis, asthma is the next most common chronic chest disease in Ibadan. While many features of the disease are similar to those seen elsewhere, skin tests indicate that ascaris is the most common antigen associated with asthma, followed by Dermatophagoides pteronyssinus."}
{"id": "PMID:475429", "title": "Carbamazepine dose-frequency requirement in children.", "content": "The dose-frequency requirement for carbamazepine (CBZ) in children was investigated using serial saliva samples to determine the daily fluctuation in drug levels. Mixed saliva was collected from 6 children (aged between 6 and 13 years) in a steady state, on each of two different dose-frequency resulted in smaller fluctuations in saliva concentration and a shorter time with levels outside the therapeutic range. Toxic features and convulsions appeared to be related to peak and trough concentrations. There was no apparent relationship between the total dose and the mean saliva concentration. The saliva CBZ half-lives in 2 children were 7.3 and 12.7 hours, and the apparent volumes of distribution (saliva) were 1.6 and 1.5 l/kg respectively. Saliva CBZ concentrations are an efficient and convenient means of tailoring individual dosage, and can be used to provide the pharmacokinetic data that rational prescribing demands.", "contents": "Carbamazepine dose-frequency requirement in children. The dose-frequency requirement for carbamazepine (CBZ) in children was investigated using serial saliva samples to determine the daily fluctuation in drug levels. Mixed saliva was collected from 6 children (aged between 6 and 13 years) in a steady state, on each of two different dose-frequency resulted in smaller fluctuations in saliva concentration and a shorter time with levels outside the therapeutic range. Toxic features and convulsions appeared to be related to peak and trough concentrations. There was no apparent relationship between the total dose and the mean saliva concentration. The saliva CBZ half-lives in 2 children were 7.3 and 12.7 hours, and the apparent volumes of distribution (saliva) were 1.6 and 1.5 l/kg respectively. Saliva CBZ concentrations are an efficient and convenient means of tailoring individual dosage, and can be used to provide the pharmacokinetic data that rational prescribing demands."}
{"id": "PMID:475430", "title": "Osteoid osteoma and benign osteoblastoma in childhood.", "content": "Three cases of osteoid osteoma and one of benign osteoblastoma in children are described. The main complaint was severe pain which was worse at night; it was relieved by aspirin or other analgesics. The diagnosis was made on clinical and radiological grounds and was confirmed on histological examination of the central nidus removed at operation. The pain was relieved in the patients with osteoid osteoma, and it was very much less after operative removal of the benign osteoblastoma. Both conditions are probably variations of the same disease process, depending on the anatomical site and the type of bone affected.", "contents": "Osteoid osteoma and benign osteoblastoma in childhood. Three cases of osteoid osteoma and one of benign osteoblastoma in children are described. The main complaint was severe pain which was worse at night; it was relieved by aspirin or other analgesics. The diagnosis was made on clinical and radiological grounds and was confirmed on histological examination of the central nidus removed at operation. The pain was relieved in the patients with osteoid osteoma, and it was very much less after operative removal of the benign osteoblastoma. Both conditions are probably variations of the same disease process, depending on the anatomical site and the type of bone affected."}
{"id": "PMID:475431", "title": "Cushing's disease presenting with growth failure: clinical remission during cyproheptadine therapy.", "content": "A 14.7 year-old boy had almost complete suppression of growth as a result of mild pituitary-dependent Cushing's disease. There was complete clinical remission during treatment with cyproheptadine (12 mg/day) and this was maintained when treatment was stopped after 18 months.", "contents": "Cushing's disease presenting with growth failure: clinical remission during cyproheptadine therapy. A 14.7 year-old boy had almost complete suppression of growth as a result of mild pituitary-dependent Cushing's disease. There was complete clinical remission during treatment with cyproheptadine (12 mg/day) and this was maintained when treatment was stopped after 18 months."}
{"id": "PMID:475432", "title": "Rectal paracetamol in small children with fever.", "content": "37 febrile children aged between 3 months and 6 years were treated with paracetamol in a dose of 15--20 mg/kg by either oral elixir or rectal suppository. The rectal route was found to have an equal antipyretic effect and offers a practical alternative in those children for whom the oral route is not possible.20", "contents": "Rectal paracetamol in small children with fever. 37 febrile children aged between 3 months and 6 years were treated with paracetamol in a dose of 15--20 mg/kg by either oral elixir or rectal suppository. The rectal route was found to have an equal antipyretic effect and offers a practical alternative in those children for whom the oral route is not possible.20"}
{"id": "PMID:475433", "title": "Acute infantile thrombocytosis and vitamin K deficiency associated with intracranial haemorrhage.", "content": "A 5-week-old breast-fed girl presented unconscious and convulsing with heavily blood-stained CSF under increased pressure. This was associated with oozing from venepuncture sites, a grossly prolonged prothrombin time, and a raised platelet count. The prothrombin time became normal 18 hours after she had been given parenteral vitamin K and a transfusion of fresh plasma and red cells, but the thrombocytosis persisted. A moderate communicating hydrocephalus also remained and was treated, and one year later health and development appeared normal. The thrombocytosis resolved after a few weeks and has not recurred.", "contents": "Acute infantile thrombocytosis and vitamin K deficiency associated with intracranial haemorrhage. A 5-week-old breast-fed girl presented unconscious and convulsing with heavily blood-stained CSF under increased pressure. This was associated with oozing from venepuncture sites, a grossly prolonged prothrombin time, and a raised platelet count. The prothrombin time became normal 18 hours after she had been given parenteral vitamin K and a transfusion of fresh plasma and red cells, but the thrombocytosis persisted. A moderate communicating hydrocephalus also remained and was treated, and one year later health and development appeared normal. The thrombocytosis resolved after a few weeks and has not recurred."}
{"id": "PMID:475434", "title": "Premature menarche without other evidence of precocious puberty.", "content": "We describe 4 young girls with recurrent vaginal bleeding in the absence of other signs of precocious sexual development. Investigation showed low oestrogen levels in 2 of them, and basal gonadotrophins were in the upper part of the prepubertal range. We believe that the isolated early menstruation in these patients was possibly related to increased sensitivity of the endometrium to oestrogens.", "contents": "Premature menarche without other evidence of precocious puberty. We describe 4 young girls with recurrent vaginal bleeding in the absence of other signs of precocious sexual development. Investigation showed low oestrogen levels in 2 of them, and basal gonadotrophins were in the upper part of the prepubertal range. We believe that the isolated early menstruation in these patients was possibly related to increased sensitivity of the endometrium to oestrogens."}
{"id": "PMID:475435", "title": "A boy with low-TSH hypothyroidism.", "content": "A case of long-standing mild hypothyroidism is described. This was caused by partial TSH deficiency probably of hypothalamic origin, with no other pituitary hormone deficiencies, although with a decreased response of FSH and LH to LHRH.", "contents": "A boy with low-TSH hypothyroidism. A case of long-standing mild hypothyroidism is described. This was caused by partial TSH deficiency probably of hypothalamic origin, with no other pituitary hormone deficiencies, although with a decreased response of FSH and LH to LHRH."}
{"id": "PMID:475436", "title": "Ring 20 chromosome in a child with seizures, minor anomalies, and retardation.", "content": "A patient is reported with seizures, developmental delay, and minor physical anomalies. Karyotype showed a ring formation of chromosome number 20. Previously reported patients with this chromosomal aberration have typically had seizures and behavioural disorders with considerable variation in the degree of physical abnormality and mental retardation. A correct diagnosis in such a case is important for accurate genetic counselling.", "contents": "Ring 20 chromosome in a child with seizures, minor anomalies, and retardation. A patient is reported with seizures, developmental delay, and minor physical anomalies. Karyotype showed a ring formation of chromosome number 20. Previously reported patients with this chromosomal aberration have typically had seizures and behavioural disorders with considerable variation in the degree of physical abnormality and mental retardation. A correct diagnosis in such a case is important for accurate genetic counselling."}
{"id": "PMID:475440", "title": "Penetration of various corticosteroids through epidermis in vitro.", "content": "The penetration through the epidermis in vitro of various topically used corticosteroids was compared. The amount penetrating through the epidermis from an ethanolic solution showed good correlation with the polarity of the corticosteroids under study. Hydrocortisone, the most polar corticosteroid, penetrates the epidermis the most rapidly; clobetasone butyrate, the least polar, the slowest. Other corticosteroids, i.e., hydrocortisone-17-butyrate, triamcinolone acetonide, and clobetasol-17-propionate, form an intermediate group whose penetration rates and polarities decrease in the indicated sequence. The corticosteroid generally accepted as having greater clinical efficacy in creams or ointments did not permeate better from an ethanolic solution in vitro.", "contents": "Penetration of various corticosteroids through epidermis in vitro. The penetration through the epidermis in vitro of various topically used corticosteroids was compared. The amount penetrating through the epidermis from an ethanolic solution showed good correlation with the polarity of the corticosteroids under study. Hydrocortisone, the most polar corticosteroid, penetrates the epidermis the most rapidly; clobetasone butyrate, the least polar, the slowest. Other corticosteroids, i.e., hydrocortisone-17-butyrate, triamcinolone acetonide, and clobetasol-17-propionate, form an intermediate group whose penetration rates and polarities decrease in the indicated sequence. The corticosteroid generally accepted as having greater clinical efficacy in creams or ointments did not permeate better from an ethanolic solution in vitro."}
{"id": "PMID:475441", "title": "Ultracytochemical study on eccrine acrosyringium of human embryos. Acid phosphatase activity during formation of lumen.", "content": "Ultracytochemical demonstration of acid phosphatase (ACPase) activity during the developing of intraepidermal eccrine sweat duct (eccrine acrosyringium) of human embryos was performed in order to elucidate the functional relationship between multivesicular dense bodies and intracellular cavities formed within the inner cells of eccrine sweat apparatus anlagen and to clarify the ultracytochemical characteristics of the multivesicular dense bodies. ACPase activity was characteristically detected in the unit membrane structures of small pinched-off vesicles within cavities and of immature microvilli lining these cavities as well as in those of multivesicular dense bodies. These findings give strong support to the theory that the multivesicular dense bodies are lysosomes and through their action the autolytic formation of intraepidermal eccrine sweat duct is carried out.", "contents": "Ultracytochemical study on eccrine acrosyringium of human embryos. Acid phosphatase activity during formation of lumen. Ultracytochemical demonstration of acid phosphatase (ACPase) activity during the developing of intraepidermal eccrine sweat duct (eccrine acrosyringium) of human embryos was performed in order to elucidate the functional relationship between multivesicular dense bodies and intracellular cavities formed within the inner cells of eccrine sweat apparatus anlagen and to clarify the ultracytochemical characteristics of the multivesicular dense bodies. ACPase activity was characteristically detected in the unit membrane structures of small pinched-off vesicles within cavities and of immature microvilli lining these cavities as well as in those of multivesicular dense bodies. These findings give strong support to the theory that the multivesicular dense bodies are lysosomes and through their action the autolytic formation of intraepidermal eccrine sweat duct is carried out."}
{"id": "PMID:475443", "title": "[Traumatically induced junctional activity of nevocellular nevi (author's transl)].", "content": "Forty papillomatous nevi, mainly with junctional nests, were partially removed surgically in such a way that a small margin was left intact on one side and the deep nevus cells were retained on the flor of the wound. After healing of the wound, a small layer of scar tissue separated the regenerated epidermis from the deep nevus cells, which were left in the dermis. After very differing intervals, as early as 30 days after the partial surgical removal, typical alterations could be observed in the epidermis above the cicatrical tissue. Most frequently, there was a hyperpigmentation with an increase in the number of basal fluorescing dendritic cells, which could also be found along the uppermost parts of epidermal appendages. Moreover, nest-like conglomerations of pigment-producing cells appeared in the form of junctional nevus cell nests. About 2-3 month after the partial surgical removal \"Abtropfung\" was visible, indicating a regeneration of the nevus. Fluorescence-histochemical and enzyme-histochemical results revealed that marked alterations can be found not only in the newly formed epidermis above the scar, which morphologically show minor alterations.", "contents": "[Traumatically induced junctional activity of nevocellular nevi (author's transl)]. Forty papillomatous nevi, mainly with junctional nests, were partially removed surgically in such a way that a small margin was left intact on one side and the deep nevus cells were retained on the flor of the wound. After healing of the wound, a small layer of scar tissue separated the regenerated epidermis from the deep nevus cells, which were left in the dermis. After very differing intervals, as early as 30 days after the partial surgical removal, typical alterations could be observed in the epidermis above the cicatrical tissue. Most frequently, there was a hyperpigmentation with an increase in the number of basal fluorescing dendritic cells, which could also be found along the uppermost parts of epidermal appendages. Moreover, nest-like conglomerations of pigment-producing cells appeared in the form of junctional nevus cell nests. About 2-3 month after the partial surgical removal \"Abtropfung\" was visible, indicating a regeneration of the nevus. Fluorescence-histochemical and enzyme-histochemical results revealed that marked alterations can be found not only in the newly formed epidermis above the scar, which morphologically show minor alterations."}
{"id": "PMID:475444", "title": "[Perforating granulomatous folliculitis in Becker's nevus (author's transl)].", "content": "Acneform lesions strictly confined to a Becker's nevus were observed in a 18-year-old man. Histologically, a granulomatous folliculitis with partial destruction of the epithelial root sheath and follicular cysts with focal penetration of keratin into the surrounding dermis were present, ruling out acne vulgaris. The concept of Becker's nevus as a complex nevogenic process, affecting ectodermal, neuroectodermal, and mesenchymal structures, also in terms of dysfunction of the pilary complex, is suggested.", "contents": "[Perforating granulomatous folliculitis in Becker's nevus (author's transl)]. Acneform lesions strictly confined to a Becker's nevus were observed in a 18-year-old man. Histologically, a granulomatous folliculitis with partial destruction of the epithelial root sheath and follicular cysts with focal penetration of keratin into the surrounding dermis were present, ruling out acne vulgaris. The concept of Becker's nevus as a complex nevogenic process, affecting ectodermal, neuroectodermal, and mesenchymal structures, also in terms of dysfunction of the pilary complex, is suggested."}
{"id": "PMID:475445", "title": "Nature of collagen in dermatofibrosarcoma protuberans.", "content": "The nature of collagen from 2 cases of dermatofibrosarcoma protuberans was studied. For this purpose, the tumor tissue was carefully separated from adjacent normal dermis. The collagen types comprised in the tumor were identified by CM-cellulose chromatographic and SDS-gel electrophoretic analysis of the component alpha-chains. Semiquantitative evaluation of the relative type III content was established by separation of the cyanogen bromide peptides on gels of 12% polyacrylamide in SDS. These studies showed that dermatofibrosarcoma protuberans contains alpha 1(I)-, alpha 2-, and alpha 1(III)-chains as well, and corresponding type I- and type III-related CNBr peptides. Comparing the collagen from dermatofibrosarcoma protuberans to that of normal skin, the relatively increased type III content in the case of dermatofibrosarcoma protuberans becomes apparent.", "contents": "Nature of collagen in dermatofibrosarcoma protuberans. The nature of collagen from 2 cases of dermatofibrosarcoma protuberans was studied. For this purpose, the tumor tissue was carefully separated from adjacent normal dermis. The collagen types comprised in the tumor were identified by CM-cellulose chromatographic and SDS-gel electrophoretic analysis of the component alpha-chains. Semiquantitative evaluation of the relative type III content was established by separation of the cyanogen bromide peptides on gels of 12% polyacrylamide in SDS. These studies showed that dermatofibrosarcoma protuberans contains alpha 1(I)-, alpha 2-, and alpha 1(III)-chains as well, and corresponding type I- and type III-related CNBr peptides. Comparing the collagen from dermatofibrosarcoma protuberans to that of normal skin, the relatively increased type III content in the case of dermatofibrosarcoma protuberans becomes apparent."}
{"id": "PMID:475446", "title": "A combined chemical-physical treatment for herpes simplex lesions in guinea pigs.", "content": "Determination of the effectiveness of chemical treatments for Herpes simplex type 2 infections has generally relied on a reduction in the rate of mortality. A more natural progression of the disease is seen when non-progressive cutaneous lesions are induced. Treatment of characteristic cutaneous lesions with ultrasound alone, a cream compound designated Herpigon alone, or a combination of ultrasound and Herpigon were compared to determine the time required for lesions to heal and the amount of virus recoverable from treated lesions. Results indicate that the use of Herpigon alone or in combination with ultrasound resulted in a significant reduction in the time required for the lesions to heal. A comparison of the amount of virus present in treated lesions indicated that the combined treatment was most effective in reducing the amount of recoverable virus.", "contents": "A combined chemical-physical treatment for herpes simplex lesions in guinea pigs. Determination of the effectiveness of chemical treatments for Herpes simplex type 2 infections has generally relied on a reduction in the rate of mortality. A more natural progression of the disease is seen when non-progressive cutaneous lesions are induced. Treatment of characteristic cutaneous lesions with ultrasound alone, a cream compound designated Herpigon alone, or a combination of ultrasound and Herpigon were compared to determine the time required for lesions to heal and the amount of virus recoverable from treated lesions. Results indicate that the use of Herpigon alone or in combination with ultrasound resulted in a significant reduction in the time required for the lesions to heal. A comparison of the amount of virus present in treated lesions indicated that the combined treatment was most effective in reducing the amount of recoverable virus."}
{"id": "PMID:475447", "title": "The lipometre: an easy tool for rapid quantitation of skin surface lipids (SSL) in man.", "content": "The purpose of this study is to present a rapid, simple, and accurate method for quantitation of SSL in man. The technique which is derived from the works by Schaefer and Kuhn-Bussius, employs a series of clearn ground glass plates, and an apparatus, the Lipometre, for the determination of the variation of light transmission across the plates as a function of the fat deposited on them. Results on 32 adults, males and females, show that the first four iterative samplings, at constant pressure, on the same forehead area follow an identical physical law independently of whether the skin is fatty or dry. This law affords a precise definition of the Casual-Level, its quantification and its correlation with skin patterns. It is consequently possible, either as an investigative or a routine technique, to predict the total amount of SSL with good accuracy within 2 min. The SSL, for non-pathological skins, fall within the range 150 microgram/cm2 to 900 microgram/cm2 which is in good agreement with values quoted in the literature.", "contents": "The lipometre: an easy tool for rapid quantitation of skin surface lipids (SSL) in man. The purpose of this study is to present a rapid, simple, and accurate method for quantitation of SSL in man. The technique which is derived from the works by Schaefer and Kuhn-Bussius, employs a series of clearn ground glass plates, and an apparatus, the Lipometre, for the determination of the variation of light transmission across the plates as a function of the fat deposited on them. Results on 32 adults, males and females, show that the first four iterative samplings, at constant pressure, on the same forehead area follow an identical physical law independently of whether the skin is fatty or dry. This law affords a precise definition of the Casual-Level, its quantification and its correlation with skin patterns. It is consequently possible, either as an investigative or a routine technique, to predict the total amount of SSL with good accuracy within 2 min. The SSL, for non-pathological skins, fall within the range 150 microgram/cm2 to 900 microgram/cm2 which is in good agreement with values quoted in the literature."}
{"id": "PMID:475448", "title": "The diffuse and macular atrophic dermatosis. A precocious and progressive light degenerative condition.", "content": "Two brothers with the \"diffuse and macular atrophic dermatosis\" making a total of 4 patients are reported. The familial occurrence, numerous large \"peau citr\u00e9ine\" plaques situated on the dorsum of the upper trunk and congenital phimosis with superficial atrophy of glans penis, represent and additional features reported. The light and electron microscopic findings of the least affected site on the arm, of a \"peau citr\u00e9ine\" and of an erythemoscleroatrophic plaque, none previously studied, have been reported. Among other items, it was also found that the elastic stain-positive globe-like structures found in an unusually large number throughout the edematous upper dermis represent more advancely transformed \"Civatte bodies\" containing epidermal filamentous cell material.", "contents": "The diffuse and macular atrophic dermatosis. A precocious and progressive light degenerative condition. Two brothers with the \"diffuse and macular atrophic dermatosis\" making a total of 4 patients are reported. The familial occurrence, numerous large \"peau citr\u00e9ine\" plaques situated on the dorsum of the upper trunk and congenital phimosis with superficial atrophy of glans penis, represent and additional features reported. The light and electron microscopic findings of the least affected site on the arm, of a \"peau citr\u00e9ine\" and of an erythemoscleroatrophic plaque, none previously studied, have been reported. Among other items, it was also found that the elastic stain-positive globe-like structures found in an unusually large number throughout the edematous upper dermis represent more advancely transformed \"Civatte bodies\" containing epidermal filamentous cell material."}
{"id": "PMID:475449", "title": "[Objective measurement of skin colour with the help of spectral photometry (author's transl)].", "content": "The colour of the human skin can be measured exactly by a new equipment which is described here. The method is based on the analysis of the reflexionproperties of the skin in the region of the visible light. On the basis of the measured reflexionproperties the colour of a probe can be computed as a function of physical parameters.", "contents": "[Objective measurement of skin colour with the help of spectral photometry (author's transl)]. The colour of the human skin can be measured exactly by a new equipment which is described here. The method is based on the analysis of the reflexionproperties of the skin in the region of the visible light. On the basis of the measured reflexionproperties the colour of a probe can be computed as a function of physical parameters."}
{"id": "PMID:475450", "title": "Quantitative thin layer chromatography for the analysis of skin surface lipids. A time-saving method using a new TLC plate.", "content": "Recently a new thin layer chromatography plate (Whatman LK 6D) became available which is extremely easy to handle and permits highly reproducible qualitative and quantitative analysis. This plate proved to be of great value for the investigation of skin surface lipids. The use of a fatty acid methyl ester as an internal standard makes it unnecessary to employ additional gravimetrical or photometrical methods for quanitative lipid analysis. The method presented in this paper is simpler and requires much less time than alternative procedures and allows a large number of lipid samples to be processed simultaneously.", "contents": "Quantitative thin layer chromatography for the analysis of skin surface lipids. A time-saving method using a new TLC plate. Recently a new thin layer chromatography plate (Whatman LK 6D) became available which is extremely easy to handle and permits highly reproducible qualitative and quantitative analysis. This plate proved to be of great value for the investigation of skin surface lipids. The use of a fatty acid methyl ester as an internal standard makes it unnecessary to employ additional gravimetrical or photometrical methods for quanitative lipid analysis. The method presented in this paper is simpler and requires much less time than alternative procedures and allows a large number of lipid samples to be processed simultaneously."}
{"id": "PMID:475451", "title": "[Effect of dermatocorticoids on the activity of hepatic tryptophane pyrrolase in the guinea pig (author's transl)].", "content": "The enzyme activity of tryptophane pyrrolase in guinea pig liver can be measurably increased--as in other mammalian species--by systemic administration of corticoids, but this effect is short-lived and achieved only with high s.c. doses of, e.g., prednisolone (Ultracorten-H-hydrosoluble). In contradistinction and rather surprisingly the enzyme activity is variably  but markedly reduced by high-dosed and protracted epicutaneous application (10 times within 2 weeks) of the well-known dermatocorticoids: hydrocortisone, fluocinolone acetonide, and clobetasol propionate. This reaction must be explained by the capacity of these corticoids to inhibit, after their percutaneous absorption, the functional axis pituitary--adrenal cortex--liver, the stimulatory role of which is mandatory for the endogenous basic activity of hepatic tryptophane pyrrolase.", "contents": "[Effect of dermatocorticoids on the activity of hepatic tryptophane pyrrolase in the guinea pig (author's transl)]. The enzyme activity of tryptophane pyrrolase in guinea pig liver can be measurably increased--as in other mammalian species--by systemic administration of corticoids, but this effect is short-lived and achieved only with high s.c. doses of, e.g., prednisolone (Ultracorten-H-hydrosoluble). In contradistinction and rather surprisingly the enzyme activity is variably  but markedly reduced by high-dosed and protracted epicutaneous application (10 times within 2 weeks) of the well-known dermatocorticoids: hydrocortisone, fluocinolone acetonide, and clobetasol propionate. This reaction must be explained by the capacity of these corticoids to inhibit, after their percutaneous absorption, the functional axis pituitary--adrenal cortex--liver, the stimulatory role of which is mandatory for the endogenous basic activity of hepatic tryptophane pyrrolase."}
{"id": "PMID:475452", "title": "Sister chromatid exchange (SCE) in human lymphocytes. Effect of UV C irradiation and age.", "content": "The number of sister chromatid exchanges (SCE) per metaphase was determined in normal human lymphocytes by the Hoechst-Giemsa procedure. UV C irradiation promotes SCE dose-dependent in the range of 0-40 mJ/cm2. No difference of this phenomenon may be observed, compairing young volunteers (20-40 years) with a 60-80-year-old group.", "contents": "Sister chromatid exchange (SCE) in human lymphocytes. Effect of UV C irradiation and age. The number of sister chromatid exchanges (SCE) per metaphase was determined in normal human lymphocytes by the Hoechst-Giemsa procedure. UV C irradiation promotes SCE dose-dependent in the range of 0-40 mJ/cm2. No difference of this phenomenon may be observed, compairing young volunteers (20-40 years) with a 60-80-year-old group."}
{"id": "PMID:475453", "title": "Immunoelectron microscopic studies in IgA linear dermatosis.", "content": "The authors investigated three cases of IgA linear dermatosis by immunoelectron microscopy. In two of the cases there were additionally some IgG deposits in the basement membrane zone. The arrangement of the IgA deposits was found to vary. In two cases it was of the dermal and the lamina lucida type at the same time, and in one case it was so close to basal cell membranes as to leave an electronlucent space on the side facing the basal lamina. In the third case, the arrangement (only IgG and complement were studied) was exclusively of the dermal type. In one of the cases the deposits had first, i.e., 1 1/2 years earlier, been confined to the lamina lucida, whereas now they were also seen below the basal lamina, although the clinical condition of the patient had remained unchanged. It would seem that the localization of IgA deposits in the basement membrane zone may vary, depending on the evolution of the disease.", "contents": "Immunoelectron microscopic studies in IgA linear dermatosis. The authors investigated three cases of IgA linear dermatosis by immunoelectron microscopy. In two of the cases there were additionally some IgG deposits in the basement membrane zone. The arrangement of the IgA deposits was found to vary. In two cases it was of the dermal and the lamina lucida type at the same time, and in one case it was so close to basal cell membranes as to leave an electronlucent space on the side facing the basal lamina. In the third case, the arrangement (only IgG and complement were studied) was exclusively of the dermal type. In one of the cases the deposits had first, i.e., 1 1/2 years earlier, been confined to the lamina lucida, whereas now they were also seen below the basal lamina, although the clinical condition of the patient had remained unchanged. It would seem that the localization of IgA deposits in the basement membrane zone may vary, depending on the evolution of the disease."}
{"id": "PMID:475454", "title": "[On the physiology of the growth of nail plate (author's transl)].", "content": "By following up histologically a localized storage of melanin in certain layers of the nail plate the differing theories on the cellular kinetics of the growth of human nail plate are checked up. Thus it turns out that the hitherto existing and even contradicting conceptions are not helpful to interpret uniquely the morphological phenomena of the concrete case. A plausible modification is presented.", "contents": "[On the physiology of the growth of nail plate (author's transl)]. By following up histologically a localized storage of melanin in certain layers of the nail plate the differing theories on the cellular kinetics of the growth of human nail plate are checked up. Thus it turns out that the hitherto existing and even contradicting conceptions are not helpful to interpret uniquely the morphological phenomena of the concrete case. A plausible modification is presented."}
{"id": "PMID:475455", "title": "Sialyltransferase levels and sialic acid concentrations in sera of patients with malignant melanomas.", "content": "Serum levels of sialyltransferase and sialic acid were measured in patients with malignant melanomas (n = 49), healthy control persons (n = 20), and patients with non-malignant skin disorders (n = 30). Both parameters were found to be higher in malignant melanoma patients than in healthy control persons, but they were not significantly higher in melanoma patients than in patients with benign skin disorders, unless widespread dissemination of metastases had occurred. The highest values were measured in patients with liver and lung metastases. No general correlation was found between sialyltransferase activities and sialic acid concentrations. Sialic acid concentrations seem to be a better index for tumor spreading than sialyltransferase activities. In early stages of the disease, shedding from tumor cells is not the major source of elevated serum levels of sialyltransferase and sialic acid, respectively.", "contents": "Sialyltransferase levels and sialic acid concentrations in sera of patients with malignant melanomas. Serum levels of sialyltransferase and sialic acid were measured in patients with malignant melanomas (n = 49), healthy control persons (n = 20), and patients with non-malignant skin disorders (n = 30). Both parameters were found to be higher in malignant melanoma patients than in healthy control persons, but they were not significantly higher in melanoma patients than in patients with benign skin disorders, unless widespread dissemination of metastases had occurred. The highest values were measured in patients with liver and lung metastases. No general correlation was found between sialyltransferase activities and sialic acid concentrations. Sialic acid concentrations seem to be a better index for tumor spreading than sialyltransferase activities. In early stages of the disease, shedding from tumor cells is not the major source of elevated serum levels of sialyltransferase and sialic acid, respectively."}
{"id": "PMID:475459", "title": "Elevated sodium levels in the public drinking water as a contributor to elevated blood pressure levels in the community.", "content": "Further statistical analyses of previously reported preliminary data upheld results showing elevated levels of sodium in drinking water may exert an adverse health effect on normal, healthy persons. High school sophomores residing in a community with elevated levels of sodium in the drinking water (107 mg/l) exhibited a marked upward shift in blood pressure distribution patterns for systolic and diastolic pressure, when compared with students in an appropriately matched community (8 mg/l). Females exhibited a blood pressure distribution pattern characteristic of persons 10 yr older, while for males the upshift was similar to that of a group approximately 2 yr older.", "contents": "Elevated sodium levels in the public drinking water as a contributor to elevated blood pressure levels in the community. Further statistical analyses of previously reported preliminary data upheld results showing elevated levels of sodium in drinking water may exert an adverse health effect on normal, healthy persons. High school sophomores residing in a community with elevated levels of sodium in the drinking water (107 mg/l) exhibited a marked upward shift in blood pressure distribution patterns for systolic and diastolic pressure, when compared with students in an appropriately matched community (8 mg/l). Females exhibited a blood pressure distribution pattern characteristic of persons 10 yr older, while for males the upshift was similar to that of a group approximately 2 yr older."}
{"id": "PMID:475460", "title": "Effects of electric arc welding on ventilatory lung function.", "content": "Respiratory symptoms, spirometry, forced expiratory flows, and the nitrogen closing volume test were studied in 119 welders and 90 controls, matched with respect to age, height, and smoking habits. Respiratory symptoms according to a questionnaire were more prevalent in the welders. No short-term changes of the measured variables during the day or week attributable to welding were found in twenty-one nonsmoking welders. Compared to the controls, closing volume and closing capacity (i. e., closing volume + residual volume) were significantly higher, and total lung capacity and the amplitude of the cardiogenic oscillations in the nitrogen curve were significantly lower in the welders who were nonsmokers or exsmokers, whereas there were no differences among smokers. These findings in welders may be attributable to deposition of welding fume particles in peripheral small airways or alveoli.", "contents": "Effects of electric arc welding on ventilatory lung function. Respiratory symptoms, spirometry, forced expiratory flows, and the nitrogen closing volume test were studied in 119 welders and 90 controls, matched with respect to age, height, and smoking habits. Respiratory symptoms according to a questionnaire were more prevalent in the welders. No short-term changes of the measured variables during the day or week attributable to welding were found in twenty-one nonsmoking welders. Compared to the controls, closing volume and closing capacity (i. e., closing volume + residual volume) were significantly higher, and total lung capacity and the amplitude of the cardiogenic oscillations in the nitrogen curve were significantly lower in the welders who were nonsmokers or exsmokers, whereas there were no differences among smokers. These findings in welders may be attributable to deposition of welding fume particles in peripheral small airways or alveoli."}
{"id": "PMID:475461", "title": "In vitro mercury uptake by human acatalasemic erythrocytes.", "content": "Human acatalasemic erythrocytes had only 0.01 to 0.06X the uptake found in normal erythrocytes with hydrogen peroxide, and 0.06 to 0.24X the uptake without hydrogen peroxide in regard to their ability of in vitro mercury uptake from air saturated with mercury vapor. Normal erythrocytes with hydrogen peroxide took up 9.3 to 37.4X more mercury than cells without hydrogen peroxide, whereas acatalesemic cells with hydrogen peroxide took up 2.4 to 5.8X more mercury than cells without hydrogen peroxide. Similar results were obtained from normal and acatalasemic hemolysates. In addition, the concentration of hydrogen peroxide affected the uptake of mercury by both normal and acatalasemic erythrocytes. Data indicate that the catalase-hydrogen peroxide system plays a role in mercury uptake, presumably by mercury oxidation, since oxidation states of the mercury were never determined in these studies.", "contents": "In vitro mercury uptake by human acatalasemic erythrocytes. Human acatalasemic erythrocytes had only 0.01 to 0.06X the uptake found in normal erythrocytes with hydrogen peroxide, and 0.06 to 0.24X the uptake without hydrogen peroxide in regard to their ability of in vitro mercury uptake from air saturated with mercury vapor. Normal erythrocytes with hydrogen peroxide took up 9.3 to 37.4X more mercury than cells without hydrogen peroxide, whereas acatalesemic cells with hydrogen peroxide took up 2.4 to 5.8X more mercury than cells without hydrogen peroxide. Similar results were obtained from normal and acatalasemic hemolysates. In addition, the concentration of hydrogen peroxide affected the uptake of mercury by both normal and acatalasemic erythrocytes. Data indicate that the catalase-hydrogen peroxide system plays a role in mercury uptake, presumably by mercury oxidation, since oxidation states of the mercury were never determined in these studies."}
{"id": "PMID:475462", "title": "Lead contamination of urban snow.", "content": "Lead content of newly fallen snow in an urban area ranges from 34 to 56 ppb. After falling, snow may incorporate major additional amounts of lead by dry deposition of lead aerosols from local sources. The highest concentration found was 2,700 ppb. Ingestion of lead-contaminated snow might pose a health hazard to inner city children.", "contents": "Lead contamination of urban snow. Lead content of newly fallen snow in an urban area ranges from 34 to 56 ppb. After falling, snow may incorporate major additional amounts of lead by dry deposition of lead aerosols from local sources. The highest concentration found was 2,700 ppb. Ingestion of lead-contaminated snow might pose a health hazard to inner city children."}
{"id": "PMID:475463", "title": "Acute arsine posioning in two workers cleaning a clogged drain.", "content": "On February 6, 1978, two maintenance workers employed by a chemical company in Atlanta, Georgia, became ill after cleaning a clogged drain. Both were hospitalized with acute fulminant hemolytic anemia and renal failure. While the clinical picture suggested arsine or stibine poisoning, preliminary investigation of the plant revealed no obvious source of arsenic, antimony, or hydrogen gas. During the cleaning operation, the men drained a mixing tank that 5 yr before had been used to store arsenical herbicides. To unclog the drain, they added a standard drain cleaner containing sodium hydroxide and aluminum chips, a combination that reacted to release hydrogen gas. This gas combined with the arsenic residue to form toxic quantities of arsine gas (arsenic trihydride). Arsenic was found in the liquid and gas from the drain and in blood and urine of both patients, and of two other workers who worked in the vicinity of the drain. This investigation suggests that drain cleaners which react to release hydrogen should not be used in situations where arsenic or antimony may be present. Furthermore, maintenance men, who may be exposed to the chemicals used in a plant, should be educated, supervised, and screened so that their risk for occupational hazards may be reduced.", "contents": "Acute arsine posioning in two workers cleaning a clogged drain. On February 6, 1978, two maintenance workers employed by a chemical company in Atlanta, Georgia, became ill after cleaning a clogged drain. Both were hospitalized with acute fulminant hemolytic anemia and renal failure. While the clinical picture suggested arsine or stibine poisoning, preliminary investigation of the plant revealed no obvious source of arsenic, antimony, or hydrogen gas. During the cleaning operation, the men drained a mixing tank that 5 yr before had been used to store arsenical herbicides. To unclog the drain, they added a standard drain cleaner containing sodium hydroxide and aluminum chips, a combination that reacted to release hydrogen gas. This gas combined with the arsenic residue to form toxic quantities of arsine gas (arsenic trihydride). Arsenic was found in the liquid and gas from the drain and in blood and urine of both patients, and of two other workers who worked in the vicinity of the drain. This investigation suggests that drain cleaners which react to release hydrogen should not be used in situations where arsenic or antimony may be present. Furthermore, maintenance men, who may be exposed to the chemicals used in a plant, should be educated, supervised, and screened so that their risk for occupational hazards may be reduced."}
{"id": "PMID:475464", "title": "Effects of nitrogen dioxide on elastin and collagen contents of lung.", "content": "Male Syrian hamsters were exposed to 30 +/- 5 ppm nitrogen dioxide for 22 hr daily for 3 wk. Nitrogen dioxide-exposed hamsters sacrificed at various times during the 3 wk exposure showed a general loss of body weight and an increased dry lung weight when compared with the controls, which were housed in a similar, but nitrogen dioxide-free environment. Analysis of total lung collagen and total lung elastin revealed a net decrease in the moieties within 4 and 10 days, respectively, following commencement of nitrogen dioxide exposure. Total lung collagen returned toward pre-exposure levels by the 14th day of nitrogen dioxide exposure. Total lung elastin did not return toward normal until termination of nitrogen dioxide exposure. Recovery in room air for 3 wk following 21 days of nitrogen dioxide exposure restored the total pulmonary collagen and elastin to valutin and collagen degradation and synthesis differ during and after nitrogen dioxide exposure. Lung collagen loss was observed earlier and was restored to normal values during the continuation of nitrogen dioxide exposure. Lung elastin loss occurred later and persisted during the entire period of exposure but returned to normal after exposure was terminated.", "contents": "Effects of nitrogen dioxide on elastin and collagen contents of lung. Male Syrian hamsters were exposed to 30 +/- 5 ppm nitrogen dioxide for 22 hr daily for 3 wk. Nitrogen dioxide-exposed hamsters sacrificed at various times during the 3 wk exposure showed a general loss of body weight and an increased dry lung weight when compared with the controls, which were housed in a similar, but nitrogen dioxide-free environment. Analysis of total lung collagen and total lung elastin revealed a net decrease in the moieties within 4 and 10 days, respectively, following commencement of nitrogen dioxide exposure. Total lung collagen returned toward pre-exposure levels by the 14th day of nitrogen dioxide exposure. Total lung elastin did not return toward normal until termination of nitrogen dioxide exposure. Recovery in room air for 3 wk following 21 days of nitrogen dioxide exposure restored the total pulmonary collagen and elastin to valutin and collagen degradation and synthesis differ during and after nitrogen dioxide exposure. Lung collagen loss was observed earlier and was restored to normal values during the continuation of nitrogen dioxide exposure. Lung elastin loss occurred later and persisted during the entire period of exposure but returned to normal after exposure was terminated."}
{"id": "PMID:475465", "title": "Human exposure to sulfur dioxide and ozone: absence of a synergistic effect.", "content": "Studies of the human health effects of exposure to a combination of ozone and sulfur dioxide have produced somewhat conflicting results; the possibility of a synergistic enhancement of toxicity when the two gases are present simultaneously remains equivocal. We evaluated the effects of 0.40 ppm sulfur dioxide, 0.40 ppm ozone, and the combination of these two under one environmental condition (25 degrees C and 45% relative humidity). Subjects alternately walked and rested during a 2-hr exposure. Subjects exposed to filtered air or to 0.40 ppm sulfur dioxide showed no significant changes in pulmonary function. When exposed to either ozone or ozone plus sulfur dioxide, significant decreases in maximum expiratory flow, forced vital capacity, and inspiratory capacity were observed. There were no significant differences in response between ozone alone and ozone plus sulfur dioxide exposures, thus, in our subjects on synergistic effects were discernible.", "contents": "Human exposure to sulfur dioxide and ozone: absence of a synergistic effect. Studies of the human health effects of exposure to a combination of ozone and sulfur dioxide have produced somewhat conflicting results; the possibility of a synergistic enhancement of toxicity when the two gases are present simultaneously remains equivocal. We evaluated the effects of 0.40 ppm sulfur dioxide, 0.40 ppm ozone, and the combination of these two under one environmental condition (25 degrees C and 45% relative humidity). Subjects alternately walked and rested during a 2-hr exposure. Subjects exposed to filtered air or to 0.40 ppm sulfur dioxide showed no significant changes in pulmonary function. When exposed to either ozone or ozone plus sulfur dioxide, significant decreases in maximum expiratory flow, forced vital capacity, and inspiratory capacity were observed. There were no significant differences in response between ozone alone and ozone plus sulfur dioxide exposures, thus, in our subjects on synergistic effects were discernible."}
{"id": "PMID:475466", "title": "Mesothelioma and exposure to mixtures of chrysotile and amphibole asbestos.", "content": "This paper provides a new analysis of previously published work and draws attention to the possibility that mixtures of amphiboles and chrysotile appear more commonly in the lungs of mesothelioma patients compared to controls than do either of the main types of fiber alone. The possibility that these results may indicate a synergistic interaction between chrysotile and the amphiboles is discussed in the light of the epidemiological data.", "contents": "Mesothelioma and exposure to mixtures of chrysotile and amphibole asbestos. This paper provides a new analysis of previously published work and draws attention to the possibility that mixtures of amphiboles and chrysotile appear more commonly in the lungs of mesothelioma patients compared to controls than do either of the main types of fiber alone. The possibility that these results may indicate a synergistic interaction between chrysotile and the amphiboles is discussed in the light of the epidemiological data."}
{"id": "PMID:475467", "title": "Airport noise and teratogenesis.", "content": "It has been suggested that exposures to high-noise levels near major airports may cause increased incidence of birth defects in the offspring of parents residing near these airports. Using data gathered in Metropolitan Atlanta during 1970 to 1972, we compared the rates of seventeen categories of defects in high- and low-noise census tracts. No significant differences were observed. However, when we subdivided the category of central nervous system defects into several subcategories of specific defects, we noted a significantly increased incidence of spina bifida without hydrocephalus in the high-noise areas. Because the small number of cases associated with this finding we did a matched case-control study using all cases of central nervous system defects born during the years 1968 to 1976. No significantly increased risk for residents in the high-noise areas was noted in this study. It is our opinion that noise or other factors associated with residence near airports are unlikely to be important environmental teratogens.", "contents": "Airport noise and teratogenesis. It has been suggested that exposures to high-noise levels near major airports may cause increased incidence of birth defects in the offspring of parents residing near these airports. Using data gathered in Metropolitan Atlanta during 1970 to 1972, we compared the rates of seventeen categories of defects in high- and low-noise census tracts. No significant differences were observed. However, when we subdivided the category of central nervous system defects into several subcategories of specific defects, we noted a significantly increased incidence of spina bifida without hydrocephalus in the high-noise areas. Because the small number of cases associated with this finding we did a matched case-control study using all cases of central nervous system defects born during the years 1968 to 1976. No significantly increased risk for residents in the high-noise areas was noted in this study. It is our opinion that noise or other factors associated with residence near airports are unlikely to be important environmental teratogens."}
{"id": "PMID:475470", "title": "Carbon monoxide: a hazard to fire fighters.", "content": "Carbon monoxide levels were measured in twenty-five fires in the city of Los Angeles to obtain information about fire fighters exposure to carbon monoxide. Levels as high as 3,000 ppm were observed for some fires. In general, when carbon monoxide levels were significantly elevated (greater than 100 ppm) the smoke was quite heavy and noxious, but in some cases the smoke was heavy and carbon monoxide levels were minimal. In two-story structures, the highest concentrations of carbon monoxide were found on the second-floor level and were usually still elevated after the fire had been \"knocked down\" but was still smoldering. These data show that fire fighters are exposed to levels of carbon monoxide which could be a serious health hazard and may be related to the high incidence of heart disease in fire fighters.", "contents": "Carbon monoxide: a hazard to fire fighters. Carbon monoxide levels were measured in twenty-five fires in the city of Los Angeles to obtain information about fire fighters exposure to carbon monoxide. Levels as high as 3,000 ppm were observed for some fires. In general, when carbon monoxide levels were significantly elevated (greater than 100 ppm) the smoke was quite heavy and noxious, but in some cases the smoke was heavy and carbon monoxide levels were minimal. In two-story structures, the highest concentrations of carbon monoxide were found on the second-floor level and were usually still elevated after the fire had been \"knocked down\" but was still smoldering. These data show that fire fighters are exposed to levels of carbon monoxide which could be a serious health hazard and may be related to the high incidence of heart disease in fire fighters."}
{"id": "PMID:475469", "title": "Polycythemia produced in rats by environmental contaminants.", "content": "Polycythemia developed in progeny from mothers who were exposed during pregnancy to a combination of methylmercury chloride plus ethylurea and sodium nitrite. The polycythemia occurred as early as one month of age and as many as 24% of the offspring developed the polycythemic condition. Many features of this condition are similar to those of polycythemia vera in man, such as elevated hematocrits and white and red blood cell counts, splenomegaly, and hyperplasia of bone marrow accompanied by megakaryocytosis.", "contents": "Polycythemia produced in rats by environmental contaminants. Polycythemia developed in progeny from mothers who were exposed during pregnancy to a combination of methylmercury chloride plus ethylurea and sodium nitrite. The polycythemia occurred as early as one month of age and as many as 24% of the offspring developed the polycythemic condition. Many features of this condition are similar to those of polycythemia vera in man, such as elevated hematocrits and white and red blood cell counts, splenomegaly, and hyperplasia of bone marrow accompanied by megakaryocytosis."}
{"id": "PMID:475471", "title": "Clinical validation of automated spirometry used in surveys of large occupational groups: comparison with conventional water spirometry.", "content": "An accurate, rugged automated spirometer which provides immediate data is useful both clinically and for screening large groups with various environmental exposures. Because the need for such surveys has been increasing, we compared a computerized mass flow-meter with a conventional water spirometer. The same forced expiration was measured by both instruments in the laboratory and during an occupational survey. Mean values for forced vital capacity (FVC) were 96 cc greater by the automated technique in the laboratory (this equals 2.2% of the value by the conventional method, with a correlation, r, of 0.998) and 97 cc greater in the field (2.3% of the conventional method; r = 0.995). Differences between the two methods in the laboratory and field for forced expiratory volume in one second (FEV 1.0) were +24 cc (0.68%; r = 0.996) and +47 cc (1.6%; r = 0.996) and for forced expiratory flow (FEF)25-75--179cc/sec (4.18%; r = 0.968) and -63 cc/sec (2.1%; r = 0.992), respectively. For FVC and FEV1.0, the differences between paired values were less than or equal to 10% of the value by water spirometry in all instances and less than or equal to 5% in 95.5% and 96% of comparisons, respectively. For FEF 25-75 the differences between paired values were less than or equal to 20% in 97.2% and less than or equal to 10% in 84.7% of comparisons. These findings confirm the validity of the measurements provided by the automated spirometer.", "contents": "Clinical validation of automated spirometry used in surveys of large occupational groups: comparison with conventional water spirometry. An accurate, rugged automated spirometer which provides immediate data is useful both clinically and for screening large groups with various environmental exposures. Because the need for such surveys has been increasing, we compared a computerized mass flow-meter with a conventional water spirometer. The same forced expiration was measured by both instruments in the laboratory and during an occupational survey. Mean values for forced vital capacity (FVC) were 96 cc greater by the automated technique in the laboratory (this equals 2.2% of the value by the conventional method, with a correlation, r, of 0.998) and 97 cc greater in the field (2.3% of the conventional method; r = 0.995). Differences between the two methods in the laboratory and field for forced expiratory volume in one second (FEV 1.0) were +24 cc (0.68%; r = 0.996) and +47 cc (1.6%; r = 0.996) and for forced expiratory flow (FEF)25-75--179cc/sec (4.18%; r = 0.968) and -63 cc/sec (2.1%; r = 0.992), respectively. For FVC and FEV1.0, the differences between paired values were less than or equal to 10% of the value by water spirometry in all instances and less than or equal to 5% in 95.5% and 96% of comparisons, respectively. For FEF 25-75 the differences between paired values were less than or equal to 20% in 97.2% and less than or equal to 10% in 84.7% of comparisons. These findings confirm the validity of the measurements provided by the automated spirometer."}
{"id": "PMID:475468", "title": "Synergism of methylmercury and selenium producing enhanced antibody formation in mice.", "content": "Mice fed 1, 5, and 10 ppm methylmercury plus 6 ppm selenium for 10 wk had a significant increase in antibody synthesis. Since methylmercury singly depresses antibody synthesis and the response was greater than that produced by selenium alone, synergism between methylmercury and selenium occurred. In this case, the synergism is considered to be advantageous to a host, while exposure by other combinations of environmental contaminants may be detrimental. Mercury concentrations in the kidney were markedly elevated when methylmercury and selenium were administered simultaneously compared to when methylmercury was given without selenium supplement. These results indicate that data collected from individual pollutants may not be of value in predicting responses to multiple exposure.", "contents": "Synergism of methylmercury and selenium producing enhanced antibody formation in mice. Mice fed 1, 5, and 10 ppm methylmercury plus 6 ppm selenium for 10 wk had a significant increase in antibody synthesis. Since methylmercury singly depresses antibody synthesis and the response was greater than that produced by selenium alone, synergism between methylmercury and selenium occurred. In this case, the synergism is considered to be advantageous to a host, while exposure by other combinations of environmental contaminants may be detrimental. Mercury concentrations in the kidney were markedly elevated when methylmercury and selenium were administered simultaneously compared to when methylmercury was given without selenium supplement. These results indicate that data collected from individual pollutants may not be of value in predicting responses to multiple exposure."}
{"id": "PMID:475473", "title": "Effect of particle size on lead absorption from the gut.", "content": "The relationship between particle size and absorption of lead particles from the gastrointestinal tract of the rat has been investigated. Preparations of metallic lead of particle size between 0 and 250 micron were incorporated in laboratory rat diets and absorption determined by measurement of tissue lead concentrations attined under standard conditions. An inverse relationship was found between particle size and lead absorption; this relationship was most marked in the 0 to 100 micron range. A five-fold enhancement of absorption was observed from the diet with lead particles of mean size 6 micron, compared with 197 micron particle size. Lead absorption from dried pain films containing lead chromate and lead octoate was measured using a similar technique. A marked enhancement of absorption was observed for both paints when particle size was reduced from 500 to 1,000 micron to less than 50 micron.", "contents": "Effect of particle size on lead absorption from the gut. The relationship between particle size and absorption of lead particles from the gastrointestinal tract of the rat has been investigated. Preparations of metallic lead of particle size between 0 and 250 micron were incorporated in laboratory rat diets and absorption determined by measurement of tissue lead concentrations attined under standard conditions. An inverse relationship was found between particle size and lead absorption; this relationship was most marked in the 0 to 100 micron range. A five-fold enhancement of absorption was observed from the diet with lead particles of mean size 6 micron, compared with 197 micron particle size. Lead absorption from dried pain films containing lead chromate and lead octoate was measured using a similar technique. A marked enhancement of absorption was observed for both paints when particle size was reduced from 500 to 1,000 micron to less than 50 micron."}
{"id": "PMID:475472", "title": "Augmentation of myocardial ischemia by low level carbon monoxide exposure in dogs.", "content": "This study was conducted to determine whether low level exposure to carbon monoxide would increase myocardial ischemia associated with acute myocardial infarction. An hour after coronary artery ligation, eleven anesthetized dogs underwent five sequential respiratory exposures to 5,000 ppm carbon monoxide, producing mean blood carboxyhemoglobin levels of 4.9% to 17.0%. Ischemia, as indicated by the amount of S-T segment elevation in epicardial electrocardiograms, increased significantly at the lowest carboxyhemoglobin level and increased further with increasing carbon monoxide exposure. These changes occurred in the absence of altered heart rate, blood pressure, left atrial pressure, cardiac output, or blood flow to ischemic myocardium. Flow to non-ischemic myocardium increased with carbon monoxide exposure, the percentage increase being approximately double the increase in carboxyhemoglobin level. Thus, low level exposure to carbon monoxide can significantly augment ischemia in acute myocardial infarction, apparently through a reduction in oxygen supplied to ischemic tissue. The data suggest that hypoxia induced by carbon monoxide exposure is more severe than can be accounted for by a simple reduction in oxygenated hemoglobin.", "contents": "Augmentation of myocardial ischemia by low level carbon monoxide exposure in dogs. This study was conducted to determine whether low level exposure to carbon monoxide would increase myocardial ischemia associated with acute myocardial infarction. An hour after coronary artery ligation, eleven anesthetized dogs underwent five sequential respiratory exposures to 5,000 ppm carbon monoxide, producing mean blood carboxyhemoglobin levels of 4.9% to 17.0%. Ischemia, as indicated by the amount of S-T segment elevation in epicardial electrocardiograms, increased significantly at the lowest carboxyhemoglobin level and increased further with increasing carbon monoxide exposure. These changes occurred in the absence of altered heart rate, blood pressure, left atrial pressure, cardiac output, or blood flow to ischemic myocardium. Flow to non-ischemic myocardium increased with carbon monoxide exposure, the percentage increase being approximately double the increase in carboxyhemoglobin level. Thus, low level exposure to carbon monoxide can significantly augment ischemia in acute myocardial infarction, apparently through a reduction in oxygen supplied to ischemic tissue. The data suggest that hypoxia induced by carbon monoxide exposure is more severe than can be accounted for by a simple reduction in oxygenated hemoglobin."}
{"id": "PMID:475479", "title": "[Hydronephrosis caused by an inferior polar vessel].", "content": "We present our statistics and results obtained in the conservative treatment of hydronephrosis in the inferior polar vessel with a kind of operation with certain modifications which we consider to be of practical interest and determined by the following premises:--Disappearance of pain after the operation. --Functional reestablishment and anatomical involution of the deformity. --Conservation of all the kidney tissue and its vascularization. --No possibility of infection due to permanent drainage and catheters.", "contents": "[Hydronephrosis caused by an inferior polar vessel]. We present our statistics and results obtained in the conservative treatment of hydronephrosis in the inferior polar vessel with a kind of operation with certain modifications which we consider to be of practical interest and determined by the following premises:--Disappearance of pain after the operation. --Functional reestablishment and anatomical involution of the deformity. --Conservation of all the kidney tissue and its vascularization. --No possibility of infection due to permanent drainage and catheters."}
{"id": "PMID:475486", "title": "Intrapleural instillation of quinacrine for treatment of recurrent spontaneous pneumothorax.", "content": "We used intrapleural instillation of quinacrine hydrochloride in 20 patients (Group A) with recurrent spontaneous pneumothorax (one bilateral) and compared their clinical course with 19 patients who underwent thoracotomy and scarification or pleurectomy (Group B) and 63 patients treated by tube thoracostomy alone (Group C). In Group A, there was one complication of treatment, a pneumothorax immediately following tube removal, which necessitated repeat tube thoracostomy, and there was one late ipsilateral recurrence 2 years after treatment. These 20 patients with 21 recurrent spontaneous pneumothoraces treated with intrapleurally administered quinacrine have been followed for from 6 months to more than 4 years with only one late recurrence on the treated side. Eight patients in Group B had postoperative complications: 2 patients who had had pleurectomy required reoperation for postoperative bleeding; lobar pneumonia developed in 3; 1 had lack of total expansion of the lung; an intrathoracic hematoma developed in 1; and an ipsilateral pneumothorax necessitating tube thoracostomy developed in 1. In Group C, the rate of recurrence of pneumothorax was 23% during the first year following treatment. Intrapleural instillation of quinacrine is a simple, low-risk, reliable, and effective treatment for recurrent spontaneous pneumothorax, and is equally as effective as thoracotomy and scarification.", "contents": "Intrapleural instillation of quinacrine for treatment of recurrent spontaneous pneumothorax. We used intrapleural instillation of quinacrine hydrochloride in 20 patients (Group A) with recurrent spontaneous pneumothorax (one bilateral) and compared their clinical course with 19 patients who underwent thoracotomy and scarification or pleurectomy (Group B) and 63 patients treated by tube thoracostomy alone (Group C). In Group A, there was one complication of treatment, a pneumothorax immediately following tube removal, which necessitated repeat tube thoracostomy, and there was one late ipsilateral recurrence 2 years after treatment. These 20 patients with 21 recurrent spontaneous pneumothoraces treated with intrapleurally administered quinacrine have been followed for from 6 months to more than 4 years with only one late recurrence on the treated side. Eight patients in Group B had postoperative complications: 2 patients who had had pleurectomy required reoperation for postoperative bleeding; lobar pneumonia developed in 3; 1 had lack of total expansion of the lung; an intrathoracic hematoma developed in 1; and an ipsilateral pneumothorax necessitating tube thoracostomy developed in 1. In Group C, the rate of recurrence of pneumothorax was 23% during the first year following treatment. Intrapleural instillation of quinacrine is a simple, low-risk, reliable, and effective treatment for recurrent spontaneous pneumothorax, and is equally as effective as thoracotomy and scarification."}
{"id": "PMID:475481", "title": "[Rhabdomyosarcoma of the spermatic cord: apropos of a case].", "content": "The rhabdomyosarcoma of the spermatic cord is a rare tumour of the genital apparatus, of which 80 cases have been published in world literature. In the present paper, we publish a case of pleomorphic rhabdomyosarcoma of the spermatic cord, pointing out the highly malignant nature of this lesion (a 10% survival rate after 5 years) as well as the delay in its diagnosis on some occasions due to confusion with hydroceles, hernias and cord cysts. As far as the treatment is concerned, all the authors agree on the extensive exeresis of the tumour, with a need for a para-aortic lymphadenectomy when there are greater divergences. In spite of these tumours being only slightly radio-sensitive, its usefulness when used locally to prevent relapses has been proved. The experience with chemiotherapy is very limited.", "contents": "[Rhabdomyosarcoma of the spermatic cord: apropos of a case]. The rhabdomyosarcoma of the spermatic cord is a rare tumour of the genital apparatus, of which 80 cases have been published in world literature. In the present paper, we publish a case of pleomorphic rhabdomyosarcoma of the spermatic cord, pointing out the highly malignant nature of this lesion (a 10% survival rate after 5 years) as well as the delay in its diagnosis on some occasions due to confusion with hydroceles, hernias and cord cysts. As far as the treatment is concerned, all the authors agree on the extensive exeresis of the tumour, with a need for a para-aortic lymphadenectomy when there are greater divergences. In spite of these tumours being only slightly radio-sensitive, its usefulness when used locally to prevent relapses has been proved. The experience with chemiotherapy is very limited."}
{"id": "PMID:475487", "title": "Surgical repair of dissection of the upper descending thoracic aorta and discrete ascending aortic aneurysm.", "content": "This report describes successful staged surgical repair in 2 patients with dissection of the upper descending thoracic aorta (DeBakey type III) with coexisting discrete Marfan's aneurysms of the ascending aorta. Initial repair of the descending aortic dissection was done through a left thoracotomy using a transverse aorta--femoral artery shunt in 1 patient and a left ventricular apex--femoral artery shunt without systemic heparinization in the other. Emphasis is placed on the need for pharmacological reduction of blood pressure during aortic cross-clamping as well as the use of a shunt to prevent dissection of the ascending aortic aneurysm. In both patients, subsequent repair of the ascending aortic aneurysm was accomplished using composite graft replacement of the aortic valve and ascending aorta. This operation is advised for such patients even in the absence of notable aortic valve incompetence.", "contents": "Surgical repair of dissection of the upper descending thoracic aorta and discrete ascending aortic aneurysm. This report describes successful staged surgical repair in 2 patients with dissection of the upper descending thoracic aorta (DeBakey type III) with coexisting discrete Marfan's aneurysms of the ascending aorta. Initial repair of the descending aortic dissection was done through a left thoracotomy using a transverse aorta--femoral artery shunt in 1 patient and a left ventricular apex--femoral artery shunt without systemic heparinization in the other. Emphasis is placed on the need for pharmacological reduction of blood pressure during aortic cross-clamping as well as the use of a shunt to prevent dissection of the ascending aortic aneurysm. In both patients, subsequent repair of the ascending aortic aneurysm was accomplished using composite graft replacement of the aortic valve and ascending aorta. This operation is advised for such patients even in the absence of notable aortic valve incompetence."}
{"id": "PMID:475483", "title": "[Urothelial tumor of the renal pelvis in horseshoe kidney].", "content": "The authors describe a case of tumour of transitional cells in patient with a horseshoe-shaped kidney, the suspected diagnosis of which was reached with the aid of a selective cinearteriography. They stress the low incidence of the same in the literature consulted which reveals the importance of studying the patients with this malformation in an attempt to prevent complications and in particular, to make an early diagnosis of a possible malignant transformation.", "contents": "[Urothelial tumor of the renal pelvis in horseshoe kidney]. The authors describe a case of tumour of transitional cells in patient with a horseshoe-shaped kidney, the suspected diagnosis of which was reached with the aid of a selective cinearteriography. They stress the low incidence of the same in the literature consulted which reveals the importance of studying the patients with this malformation in an attempt to prevent complications and in particular, to make an early diagnosis of a possible malignant transformation."}
{"id": "PMID:475488", "title": "Clinical experience with the Lillehei-Kaster valve prosthesis.", "content": "The Lillehei-Kaster valve was used in 215 patients over a 7-year period. The aortic valve was replaced in 81 of them. Hospital mortality was 21% (17 patients). Ten patients were lost to follow-up and long-term mortality was 5% (3 patients). The incidence of thromboembolism was 2.6 per 100 patient-years, and actuarial survival was 96% at 5 and 87% at 7 years. Mitral valve replacement was performed in 108 patients. Hospital mortality was 20% (22 patients), 24 patients were lost to follow-up, and long-term mortality was 13% (11 patients). The combined incidence of thromboembolism was 5.0 per 100 patient-years, and actuarial survival was 81% at 5 years and 75% at 7 years. In both groups, the majority of patients improved clinically. Fifteen patients had double-valve replacement, and 11 had coronary revascularization in addition to valve replacement.", "contents": "Clinical experience with the Lillehei-Kaster valve prosthesis. The Lillehei-Kaster valve was used in 215 patients over a 7-year period. The aortic valve was replaced in 81 of them. Hospital mortality was 21% (17 patients). Ten patients were lost to follow-up and long-term mortality was 5% (3 patients). The incidence of thromboembolism was 2.6 per 100 patient-years, and actuarial survival was 96% at 5 and 87% at 7 years. Mitral valve replacement was performed in 108 patients. Hospital mortality was 20% (22 patients), 24 patients were lost to follow-up, and long-term mortality was 13% (11 patients). The combined incidence of thromboembolism was 5.0 per 100 patient-years, and actuarial survival was 81% at 5 years and 75% at 7 years. In both groups, the majority of patients improved clinically. Fifteen patients had double-valve replacement, and 11 had coronary revascularization in addition to valve replacement."}
{"id": "PMID:475489", "title": "Effect of therapeutic-dose irradiation on left ventricular function in conscious dogs.", "content": "Every week, 8 conscious, chronically instrumented dogs underwent left ventricular (LV) function studies before, during, and after cardiac irradiation with cobalt 60 (myocardial dose of 5,000 rads at 200 rads per day through a 5 X 5 cm port). During the weekly LV function studies, left atrial pressures were raised by rapid infusion of balanced saline solution. Heart rate, aortic pressures, left and right atrial pressures, LV pressure, left ventricular end-diastolic pressure (LVEDP), and maximum rate of rise of LV pressure were recorded. Electrocardiograms were made. Cardiac outputs were obtained by thermodilution. Stroke volume, LV stroke work, and LV minute work were calculated. LV function curves were constructed each week. All dogs lost weight and became irritable after approximately 800 rads. The electrocardiograms showed signs of myocardial injury after 1,200 rads. All variables were slightly depressed during the first 8 weeks following irradiation. At the eleventh week, both left atrial pressure and LVEDP increased significantly and LV function declined. There was also clinical evidence of LV failure at rest and after volume loading. This study documents that external cardiac irradiation, in a therapeutic dose and schedule range, causes depression of LV function. These functional changes were partially reversed when the follow-up study was continued to six months after irradiation.", "contents": "Effect of therapeutic-dose irradiation on left ventricular function in conscious dogs. Every week, 8 conscious, chronically instrumented dogs underwent left ventricular (LV) function studies before, during, and after cardiac irradiation with cobalt 60 (myocardial dose of 5,000 rads at 200 rads per day through a 5 X 5 cm port). During the weekly LV function studies, left atrial pressures were raised by rapid infusion of balanced saline solution. Heart rate, aortic pressures, left and right atrial pressures, LV pressure, left ventricular end-diastolic pressure (LVEDP), and maximum rate of rise of LV pressure were recorded. Electrocardiograms were made. Cardiac outputs were obtained by thermodilution. Stroke volume, LV stroke work, and LV minute work were calculated. LV function curves were constructed each week. All dogs lost weight and became irritable after approximately 800 rads. The electrocardiograms showed signs of myocardial injury after 1,200 rads. All variables were slightly depressed during the first 8 weeks following irradiation. At the eleventh week, both left atrial pressure and LVEDP increased significantly and LV function declined. There was also clinical evidence of LV failure at rest and after volume loading. This study documents that external cardiac irradiation, in a therapeutic dose and schedule range, causes depression of LV function. These functional changes were partially reversed when the follow-up study was continued to six months after irradiation."}
{"id": "PMID:475484", "title": "[Nephrectomy in hypertensive unilateral nephropathies].", "content": "A nephrectomy has been performed on eight cases of hypertensive, unilateral nephropathies, in patients aged between 20 and 68, with a clear predominance of the female sex, namely 75% of the cases. The results were excellent and the patients maintained a normal tension for a very long period, about two years after the nephrectomy. The authors comment upon the means of diagnosis and treatment. They stress that the future of patients suffering from a unilateral, hypertensive nephrectomy depends upon the possibility of logical and correct surgery in order to prevent malignancy from developing.", "contents": "[Nephrectomy in hypertensive unilateral nephropathies]. A nephrectomy has been performed on eight cases of hypertensive, unilateral nephropathies, in patients aged between 20 and 68, with a clear predominance of the female sex, namely 75% of the cases. The results were excellent and the patients maintained a normal tension for a very long period, about two years after the nephrectomy. The authors comment upon the means of diagnosis and treatment. They stress that the future of patients suffering from a unilateral, hypertensive nephrectomy depends upon the possibility of logical and correct surgery in order to prevent malignancy from developing."}
{"id": "PMID:475490", "title": "Blood conservation during myocardial revascularization.", "content": "A prospective study of blood utilization in 50 consecutive patients undergoing elective coronary artery bypass was undertaken. Blood was removed from all patients during induction of anesthesia and reinfused after bypass (mean, 675 ml). Intraoperatively, all discard suction was routed through a regionally heparinized collecting and processing system, and the resulting red cell concentrate was transfused. At the conclusion of bypass, all blood remaining in the pump oxygenator was retained for transfusion. After operation, shed mediastinal blood was collected in a sterile, filtered collection system and transfused. Normovolemic anemia was accepted in hemodynamically stable patients. The mean amount of patients' blood salvaged by the intraoperative system was 259 ml (range, 0 to 724 ml) and by the postoperative system, 194 ml (range, 0 to 564 ml). Ninety-four percent (47/50) of the patients received no bank blood or blood products during their hospital stay. No patients received bank blood intraoperatively or during the first 24 hours following operation. There were no complications attributable to blood salvage techniques.", "contents": "Blood conservation during myocardial revascularization. A prospective study of blood utilization in 50 consecutive patients undergoing elective coronary artery bypass was undertaken. Blood was removed from all patients during induction of anesthesia and reinfused after bypass (mean, 675 ml). Intraoperatively, all discard suction was routed through a regionally heparinized collecting and processing system, and the resulting red cell concentrate was transfused. At the conclusion of bypass, all blood remaining in the pump oxygenator was retained for transfusion. After operation, shed mediastinal blood was collected in a sterile, filtered collection system and transfused. Normovolemic anemia was accepted in hemodynamically stable patients. The mean amount of patients' blood salvaged by the intraoperative system was 259 ml (range, 0 to 724 ml) and by the postoperative system, 194 ml (range, 0 to 564 ml). Ninety-four percent (47/50) of the patients received no bank blood or blood products during their hospital stay. No patients received bank blood intraoperatively or during the first 24 hours following operation. There were no complications attributable to blood salvage techniques."}
{"id": "PMID:475494", "title": "[The system of complement in active rheumatic fever].", "content": "Pathogenesis of inflammation in acute rheumatic fever is still unknown. There is humoral immune response against streptococcal products and streptococcal infection plays a role in etiopathogenesis. When inflammation is due to humoral immune mechanisms, the serum Complement system is the most important mediator. We studied functional aspects of the Complement system in nine patients suffering acute rheumatic fever, all of them with clear humoral immune response against streptococcus. We could not show any abnormalities in Complement. Probably, inflammation during acute rheumatic fever depends on Complement independent mechanisms.", "contents": "[The system of complement in active rheumatic fever]. Pathogenesis of inflammation in acute rheumatic fever is still unknown. There is humoral immune response against streptococcal products and streptococcal infection plays a role in etiopathogenesis. When inflammation is due to humoral immune mechanisms, the serum Complement system is the most important mediator. We studied functional aspects of the Complement system in nine patients suffering acute rheumatic fever, all of them with clear humoral immune response against streptococcus. We could not show any abnormalities in Complement. Probably, inflammation during acute rheumatic fever depends on Complement independent mechanisms."}
{"id": "PMID:475495", "title": "[Endocardial monophasic action potentials obtained by means of suction catheters].", "content": "Endocardial monophasic active potentials (M.A.P.) recorded by suction --tip intracardiac catheters constitute a new research-- method in cardiac arrythmias, electrophysiology and pharmacology. The characteristics of MAP at auricular and ventricular levels are analyzed. The limitations of the method in its incapacity to record the MAP of conduction tissues is discussed. This method of recording MAP is evaluated with antiarrythmic drugs of tipes I, II, III and IV. The results are identical to recordings performed with intracellular microelectrodes.", "contents": "[Endocardial monophasic action potentials obtained by means of suction catheters]. Endocardial monophasic active potentials (M.A.P.) recorded by suction --tip intracardiac catheters constitute a new research-- method in cardiac arrythmias, electrophysiology and pharmacology. The characteristics of MAP at auricular and ventricular levels are analyzed. The limitations of the method in its incapacity to record the MAP of conduction tissues is discussed. This method of recording MAP is evaluated with antiarrythmic drugs of tipes I, II, III and IV. The results are identical to recordings performed with intracellular microelectrodes."}
{"id": "PMID:475496", "title": "[Phonomechanocardiographic parameters of the ventricular function in the healthy adult].", "content": "There are studied several phonomechanocardiographic parameters of ventricular function and the obtained values in a normal patients' lot. It is analyzed the semiology of those data which offer information about pre-charge (apexcardiogramme's \"a\" index) of the myocardiac contractile state (true isosvstolic phase, ventricular pression's elevation middle velocity, integrated isovolumetric pression, and contracility index) and of its \"pump\" function (expulsion fraction); in the same way it is discussed the measurement's potential utility of diastolic intervals. It is emphasized the value that these parameters' measurement can have to know cardiac capacity and the affectation this can have by pharmacologic influence or by the ilnes.", "contents": "[Phonomechanocardiographic parameters of the ventricular function in the healthy adult]. There are studied several phonomechanocardiographic parameters of ventricular function and the obtained values in a normal patients' lot. It is analyzed the semiology of those data which offer information about pre-charge (apexcardiogramme's \"a\" index) of the myocardiac contractile state (true isosvstolic phase, ventricular pression's elevation middle velocity, integrated isovolumetric pression, and contracility index) and of its \"pump\" function (expulsion fraction); in the same way it is discussed the measurement's potential utility of diastolic intervals. It is emphasized the value that these parameters' measurement can have to know cardiac capacity and the affectation this can have by pharmacologic influence or by the ilnes."}
{"id": "PMID:475497", "title": "[Phonomechanocardiography of constrictive pericarditis].", "content": "There were studied 19 constrictive pericarditis cases demonstrated by anatomist study. It was evident, at all of them, systemic veiny hypertension's syndrome. \"Extinguished\" cardiac noises and \"quiet\" heart only appeared at the 42% of the cases. 73% of patients were found with important incapacity. Lyan's pericardic protodiastolic crack was registered at the 75% of the cases and only at 2 cases (10.9%) it was found reinforcement of pulmonary noise II. It is agree with the haemodynamic discovery of pulmonary pression's light elevation. Characteristically, precordiogrammes showed great \"A\" wave, and it was agree with telediastolic pression's elevation of the two ventricles obtained by catheterism. Phlebogramme was characteristic of systemic veiny hypertension by impediment of ventricular filled at all the studied cases. Measurement of cardiac cycle's phases showed diminution at PE, Blumberger's intrasystolic quotient, left expulsion fraction (Carrard's method) and ventricular pression's elevation middle velocity (VPEMV). By the contrary Weissler's index was found elevated. Eventhough found ciphers could be considered like bordering normal values, there is a difference statistically significance in relation with the values that were found in sane subjects. These discoveries were interpreted in the base that the patient's heart with constrictive pericarditis acts at the curve's ascendent part of the ventricular function because it has incapacity to utilize Starling's mechanism. Process' chronicity produces myocardic atrophy by \"discuss\" and, by this, ventricular function's improvement can be no immediate to pericardiectomy. Apexcardiogramme shows the impedement to ventricular filled with its diastolic morphology which is very similar to intracavitary pression's curve (\"square root's image\"). It is postuled the hypothesis that these sicks do not develop important pulmonary hypertension, because right ventricle's poor diastolic distension impides generation of major expense and systolic pression and, by other side, the impedement to ventricular filled has repercussion over right auricle and systemic veiny territory much more distensible, with the known clinical consequences.", "contents": "[Phonomechanocardiography of constrictive pericarditis]. There were studied 19 constrictive pericarditis cases demonstrated by anatomist study. It was evident, at all of them, systemic veiny hypertension's syndrome. \"Extinguished\" cardiac noises and \"quiet\" heart only appeared at the 42% of the cases. 73% of patients were found with important incapacity. Lyan's pericardic protodiastolic crack was registered at the 75% of the cases and only at 2 cases (10.9%) it was found reinforcement of pulmonary noise II. It is agree with the haemodynamic discovery of pulmonary pression's light elevation. Characteristically, precordiogrammes showed great \"A\" wave, and it was agree with telediastolic pression's elevation of the two ventricles obtained by catheterism. Phlebogramme was characteristic of systemic veiny hypertension by impediment of ventricular filled at all the studied cases. Measurement of cardiac cycle's phases showed diminution at PE, Blumberger's intrasystolic quotient, left expulsion fraction (Carrard's method) and ventricular pression's elevation middle velocity (VPEMV). By the contrary Weissler's index was found elevated. Eventhough found ciphers could be considered like bordering normal values, there is a difference statistically significance in relation with the values that were found in sane subjects. These discoveries were interpreted in the base that the patient's heart with constrictive pericarditis acts at the curve's ascendent part of the ventricular function because it has incapacity to utilize Starling's mechanism. Process' chronicity produces myocardic atrophy by \"discuss\" and, by this, ventricular function's improvement can be no immediate to pericardiectomy. Apexcardiogramme shows the impedement to ventricular filled with its diastolic morphology which is very similar to intracavitary pression's curve (\"square root's image\"). It is postuled the hypothesis that these sicks do not develop important pulmonary hypertension, because right ventricle's poor diastolic distension impides generation of major expense and systolic pression and, by other side, the impedement to ventricular filled has repercussion over right auricle and systemic veiny territory much more distensible, with the known clinical consequences."}
{"id": "PMID:475498", "title": "[Congestive myocardiopathy and early dyastolic closure of the tricuspid valve. Report of a case with acute tricuspidization].", "content": "A case with congestive cardiomiopathy and early diastolic closure of the tricuspid valve is presented. This abnormal echocardiographic finding was probably the result of severe tricuspid regurgitation occurring in a restricted right ventricle. The absence of paradoxical septum was determinedly the left ventricular pathology. In this case, echocardiography was more sensitive for the detection of the left sided abnormalities, than the heart cath data.", "contents": "[Congestive myocardiopathy and early dyastolic closure of the tricuspid valve. Report of a case with acute tricuspidization]. A case with congestive cardiomiopathy and early diastolic closure of the tricuspid valve is presented. This abnormal echocardiographic finding was probably the result of severe tricuspid regurgitation occurring in a restricted right ventricle. The absence of paradoxical septum was determinedly the left ventricular pathology. In this case, echocardiography was more sensitive for the detection of the left sided abnormalities, than the heart cath data."}
{"id": "PMID:475500", "title": "[Clinical experience with the use of dura mater prosthesis (the 1st 60 patients)].", "content": "It is presented the experience in the first 60 patients with duramater's prothesis. It is exposed the methodology which was utilized for these patients, in the same manner, the valve's confection technic. There are signaled the advantages, over mechanic valves, that these prothesis have, specially the no utilization of anticoagulatings, a minor incidence of tromboembolic phenomena, insignificant transvalvular gradient, major resistance to infection, central flux, minor turbulence and lower cost. It is informed about the obtained results with electrocardiographic, radiologic, phonomechanocardiographic, echocardiographic and haemodynamic postoperation studies, evolution after two years has been very good, 90% of the patients shows substantial improvement and only 30% need to take anticoagulants. There were 5 hospital deaths and 1 late death by hepatitis.", "contents": "[Clinical experience with the use of dura mater prosthesis (the 1st 60 patients)]. It is presented the experience in the first 60 patients with duramater's prothesis. It is exposed the methodology which was utilized for these patients, in the same manner, the valve's confection technic. There are signaled the advantages, over mechanic valves, that these prothesis have, specially the no utilization of anticoagulatings, a minor incidence of tromboembolic phenomena, insignificant transvalvular gradient, major resistance to infection, central flux, minor turbulence and lower cost. It is informed about the obtained results with electrocardiographic, radiologic, phonomechanocardiographic, echocardiographic and haemodynamic postoperation studies, evolution after two years has been very good, 90% of the patients shows substantial improvement and only 30% need to take anticoagulants. There were 5 hospital deaths and 1 late death by hepatitis."}
{"id": "PMID:475501", "title": "[Antihypertensive effect of the vasodilator minoxidil combined with dihydrochlorothiazide and propranolol in resistent hypertension].", "content": "The antihipertensive effect of a diuretic (dihidrochlorothiazide 100-200 mgr.), a betablocker (propranolol 20-180 mgr.) and the vasodilating agent minoxidil (25-70 mgr.), administered daily during 10-16 weeks, was asessed in 18 patients with refractory arterial hypertension (RAH). The arterial blood pressure (mm. of Hg.) differences obtained at the end of a 2 weeks diuretic period and at the end of the triple theraphy period was --53.1 +/- 18.1, p less than or equal to 0.001 (mean, standard deviation and significance) and --26.5 +/- 11.8, p less than or equal to 0.001, for clinostatic sistolic and diastolic pressures, respectively and --45.7 +/- 24.1, p less than or equal to 0.001 and --21.6 +/- 40 (p less than or equal to 0.001) for orthostatic sistolic and diastolic pressures, respectively. 66.6% of cases normalized the blood pressure at the end of the protocol. No significant changes were observed in body weight and pulse rate. In 94.4, 66.6 and 16.6% of cases appeared hypertricosis lanuginosa; edema or palpitations, respectively, as theraphy related symptoms. RAH is an entity of poor prognosis and difficult therapheutic management. In this study a significant antihipertensive response was achieved, which was superior to the one obtained in a similar population treated with other regimens in the same clinic. Despite the opposed attitude to continue the treatment exihibited by most female patients, we believe that minoxidil has enriched the therapheutic armamentarium of this dreadfull type of hypertension.", "contents": "[Antihypertensive effect of the vasodilator minoxidil combined with dihydrochlorothiazide and propranolol in resistent hypertension]. The antihipertensive effect of a diuretic (dihidrochlorothiazide 100-200 mgr.), a betablocker (propranolol 20-180 mgr.) and the vasodilating agent minoxidil (25-70 mgr.), administered daily during 10-16 weeks, was asessed in 18 patients with refractory arterial hypertension (RAH). The arterial blood pressure (mm. of Hg.) differences obtained at the end of a 2 weeks diuretic period and at the end of the triple theraphy period was --53.1 +/- 18.1, p less than or equal to 0.001 (mean, standard deviation and significance) and --26.5 +/- 11.8, p less than or equal to 0.001, for clinostatic sistolic and diastolic pressures, respectively and --45.7 +/- 24.1, p less than or equal to 0.001 and --21.6 +/- 40 (p less than or equal to 0.001) for orthostatic sistolic and diastolic pressures, respectively. 66.6% of cases normalized the blood pressure at the end of the protocol. No significant changes were observed in body weight and pulse rate. In 94.4, 66.6 and 16.6% of cases appeared hypertricosis lanuginosa; edema or palpitations, respectively, as theraphy related symptoms. RAH is an entity of poor prognosis and difficult therapheutic management. In this study a significant antihipertensive response was achieved, which was superior to the one obtained in a similar population treated with other regimens in the same clinic. Despite the opposed attitude to continue the treatment exihibited by most female patients, we believe that minoxidil has enriched the therapheutic armamentarium of this dreadfull type of hypertension."}
{"id": "PMID:475502", "title": "[Arteriovenous fistula and rheumatic cardiopathy].", "content": "A case of pulmonary arteriovenous fistula associated with rheumatic mitral heart disease is presented. We have reviewed the literature and no published case with such association has been described. The loud mitral insufficiency murmur hide the continuous murmur of the fistula. The angiographic study demonstrated the A-V fistula. Retrospectively the diagnosis should have been suspected due to cyanosis, slight clubbing, the radiologic findings and the important Qs/Qt shunt. The associated pulmonary embolic phenomena made more difficult the diagnosis.", "contents": "[Arteriovenous fistula and rheumatic cardiopathy]. A case of pulmonary arteriovenous fistula associated with rheumatic mitral heart disease is presented. We have reviewed the literature and no published case with such association has been described. The loud mitral insufficiency murmur hide the continuous murmur of the fistula. The angiographic study demonstrated the A-V fistula. Retrospectively the diagnosis should have been suspected due to cyanosis, slight clubbing, the radiologic findings and the important Qs/Qt shunt. The associated pulmonary embolic phenomena made more difficult the diagnosis."}
{"id": "PMID:475507", "title": "Methyldopa abolishes neurally evoked contractions of the vas deferens in vitro.", "content": "Low concentrations of methyldopa (2 microgram/ml - 20 microgram/ml) reduced neurally evoked contractions of the isolated guinea-pig and the rat vas deferens. These contractions were completely abolished when the vas was incubated with methyldopa (200 microgram/ml - 400 microgram/ml) for 1.5 hr +/- 0.1 hr (guinea-pigs) and 3.0 hr +/- 0.5 hr (rats). Vasa deferentia incubated with methyldopa became supersensitive to noradrenaline and dopamine. Vas deferentia from reserpinized animals showed reduced contractions upon electrical stimulation but became supersensitive to dopamine and noradrenaline. Reserpine pretreatment diminished the response of the vas to tyramine; however, methyldopa restored the responses to a greater magnitude suggesting the formation of methyldopamine and methylnoradrenaline. The possible mechanisms of action of methyldopa is discussed.", "contents": "Methyldopa abolishes neurally evoked contractions of the vas deferens in vitro. Low concentrations of methyldopa (2 microgram/ml - 20 microgram/ml) reduced neurally evoked contractions of the isolated guinea-pig and the rat vas deferens. These contractions were completely abolished when the vas was incubated with methyldopa (200 microgram/ml - 400 microgram/ml) for 1.5 hr +/- 0.1 hr (guinea-pigs) and 3.0 hr +/- 0.5 hr (rats). Vasa deferentia incubated with methyldopa became supersensitive to noradrenaline and dopamine. Vas deferentia from reserpinized animals showed reduced contractions upon electrical stimulation but became supersensitive to dopamine and noradrenaline. Reserpine pretreatment diminished the response of the vas to tyramine; however, methyldopa restored the responses to a greater magnitude suggesting the formation of methyldopamine and methylnoradrenaline. The possible mechanisms of action of methyldopa is discussed."}
{"id": "PMID:475508", "title": "Neuro-muscular depressant action of pindolol in comparison with propranolol and procaine.", "content": "The action of beta-adrenergic blockers on neuromuscular function has been reexamined using a recent beta-blocker pindolol (Visken), along with propranolol and procaine, on three isolated skeletal muscle preparations from the same species, frog: hyoglossus muscle stimulated through hypoglossal nerve, rectus abdominis and hyoglossus muscles using acetylcholine as an agonist. The depressant action of pindolol and propranolol was found to be non-competitive. The three test-drugs potentiated the depressant actions of d-tubocurarine and succinylcholine. The results point to a local anaesthetic mode of action of beta-blockers. Pindolol which is 10-40 times more potent than propranolol as a beta-blocker but now shown to be only one-fourth as potent in its neuro-muscular depressant action, may have lesser neuro-muscular side effects than propranolol in patients receiving high doses of propranolol for antihypertensive therapy.", "contents": "Neuro-muscular depressant action of pindolol in comparison with propranolol and procaine. The action of beta-adrenergic blockers on neuromuscular function has been reexamined using a recent beta-blocker pindolol (Visken), along with propranolol and procaine, on three isolated skeletal muscle preparations from the same species, frog: hyoglossus muscle stimulated through hypoglossal nerve, rectus abdominis and hyoglossus muscles using acetylcholine as an agonist. The depressant action of pindolol and propranolol was found to be non-competitive. The three test-drugs potentiated the depressant actions of d-tubocurarine and succinylcholine. The results point to a local anaesthetic mode of action of beta-blockers. Pindolol which is 10-40 times more potent than propranolol as a beta-blocker but now shown to be only one-fourth as potent in its neuro-muscular depressant action, may have lesser neuro-muscular side effects than propranolol in patients receiving high doses of propranolol for antihypertensive therapy."}
{"id": "PMID:475503", "title": "[Total transposition of the great vessels associated to a peculiar preexcitation syndrome].", "content": "Following the historical introduction concerning the evolution of our present knowledge of the W-P-W syndrome, we discuss a unique case of complete transposition of the great vessels with an unusual type of pre-excitation. We attributed this excitation to an uncommonly placed bundle of Kent, which, to our knowledge, has not been previously reported as coexisting with the aforementioned congenital heart malformation. A review of the literature, a discussion of other congenital heart diseases associated with disturbances of A-V conduction, as well as the clinical and para-clinical data upon which we base our diagnosis are presented.", "contents": "[Total transposition of the great vessels associated to a peculiar preexcitation syndrome]. Following the historical introduction concerning the evolution of our present knowledge of the W-P-W syndrome, we discuss a unique case of complete transposition of the great vessels with an unusual type of pre-excitation. We attributed this excitation to an uncommonly placed bundle of Kent, which, to our knowledge, has not been previously reported as coexisting with the aforementioned congenital heart malformation. A review of the literature, a discussion of other congenital heart diseases associated with disturbances of A-V conduction, as well as the clinical and para-clinical data upon which we base our diagnosis are presented."}
{"id": "PMID:475509", "title": "Purine effects at the neuromuscular junction and their modification by theophylline, imidazole and verapamil.", "content": "Interactions between some substances (theophylline, noradrenaline, imidazole, ouabain and verapamil) and adenosine or adenosine triphosphate (ATP) were examined by recording the twitch tension of partially magnesium blocked phrenic-rat diaphragm preparations stimulated indirectly. Theophylline (an inhibitor of phosphodieterases) prevented and reversed the neuromuscular depression induced either by adenosine or ATP, and these substances antagonized the neuromuscular facilitation caused by imidazole (an activator of phosphodiesterases); noradrenaline and ouabain did not modify and verapamil increased that depression. These results indicate that the putative purine presynaptic receptor is not the ATPase, that it does not appear to operate by implication of cyclic AMP, but that it could mediate a process involved in the reduction of transmitter release by regulating the entry of calcium that follows the depolorization of the motor nerve endings.", "contents": "Purine effects at the neuromuscular junction and their modification by theophylline, imidazole and verapamil. Interactions between some substances (theophylline, noradrenaline, imidazole, ouabain and verapamil) and adenosine or adenosine triphosphate (ATP) were examined by recording the twitch tension of partially magnesium blocked phrenic-rat diaphragm preparations stimulated indirectly. Theophylline (an inhibitor of phosphodieterases) prevented and reversed the neuromuscular depression induced either by adenosine or ATP, and these substances antagonized the neuromuscular facilitation caused by imidazole (an activator of phosphodiesterases); noradrenaline and ouabain did not modify and verapamil increased that depression. These results indicate that the putative purine presynaptic receptor is not the ATPase, that it does not appear to operate by implication of cyclic AMP, but that it could mediate a process involved in the reduction of transmitter release by regulating the entry of calcium that follows the depolorization of the motor nerve endings."}
{"id": "PMID:475504", "title": "[Autoimmune hemolytic anemia induced by alpha methyldopa].", "content": "We present in this paper two cases of auto-immune hemolytic anemie caused by ingestion of alpha-metildope Coombs' test positive. We suggest to make Coombs test to every patient in whose treatment has been included the use of alpha-metildope.", "contents": "[Autoimmune hemolytic anemia induced by alpha methyldopa]. We present in this paper two cases of auto-immune hemolytic anemie caused by ingestion of alpha-metildope Coombs' test positive. We suggest to make Coombs test to every patient in whose treatment has been included the use of alpha-metildope."}
{"id": "PMID:475510", "title": "Cholinergic and adrenergic innervations of the muscularis mucosae in guinea-pig esophagus.", "content": "Transmural stimulation of the isolated muscularis mucosae from guinea-pig esophagus induced contraction, which was completely inhibited by the pretreatment with atropine (o.1 microM) or tetrodotoxin (0.1 microM). In the presence of atropine, muscularis mucosae which contracted with histamine 2 microM slightly relaxed by transmural stimulation in 8 out of 20 preparations. The relaxation was inhibited by an addition of guanethidine (10 microM) or propranolol (2 microM). The vagal stimulation or an addition of nicotine also induced contractions of muscularis mucosae which were blocked by hexamethonium (20-50 microM), tetrodotoxin (0.1 microM) or atropine (0.1 microM). Using the formaldehydeinduced fluorescence technique, a few catecholamine-containing nerve fibres were observed in muscularis mucosae or in the wall of small blood vessels of guinea-pig esophagus. From these results, it is suggested that muscularis mucosae in guinea-pig esophagus is innervated mostly with excitatory cholinergic nerves, and sparsely with inhibitory adrenergic nerves, but not with non-adrenergic and non-cholinergic nerves.", "contents": "Cholinergic and adrenergic innervations of the muscularis mucosae in guinea-pig esophagus. Transmural stimulation of the isolated muscularis mucosae from guinea-pig esophagus induced contraction, which was completely inhibited by the pretreatment with atropine (o.1 microM) or tetrodotoxin (0.1 microM). In the presence of atropine, muscularis mucosae which contracted with histamine 2 microM slightly relaxed by transmural stimulation in 8 out of 20 preparations. The relaxation was inhibited by an addition of guanethidine (10 microM) or propranolol (2 microM). The vagal stimulation or an addition of nicotine also induced contractions of muscularis mucosae which were blocked by hexamethonium (20-50 microM), tetrodotoxin (0.1 microM) or atropine (0.1 microM). Using the formaldehydeinduced fluorescence technique, a few catecholamine-containing nerve fibres were observed in muscularis mucosae or in the wall of small blood vessels of guinea-pig esophagus. From these results, it is suggested that muscularis mucosae in guinea-pig esophagus is innervated mostly with excitatory cholinergic nerves, and sparsely with inhibitory adrenergic nerves, but not with non-adrenergic and non-cholinergic nerves."}
{"id": "PMID:475511", "title": "Influence of R-805 on the contractility and reactivity of rat myometrium.", "content": "It has been found that R-805 reduces and then eliminates the contractile activity of the isolated rat myometrium. R-805 weakens and then abolishes the reaction of the myometrium to polypeptides (oxytocin, vasopressin, bradykinin and hypertensin). In the presence of R-805, exogenous prostaglandin F(2) alpha restores the myometrial reactivity to the investigated polypeptides. On the basis of the obtained results and data from the literature a tentative hypothesis has been put forward as to the mechanism of R-805 action on the myometrial reactivity to polypeptides.", "contents": "Influence of R-805 on the contractility and reactivity of rat myometrium. It has been found that R-805 reduces and then eliminates the contractile activity of the isolated rat myometrium. R-805 weakens and then abolishes the reaction of the myometrium to polypeptides (oxytocin, vasopressin, bradykinin and hypertensin). In the presence of R-805, exogenous prostaglandin F(2) alpha restores the myometrial reactivity to the investigated polypeptides. On the basis of the obtained results and data from the literature a tentative hypothesis has been put forward as to the mechanism of R-805 action on the myometrial reactivity to polypeptides."}
{"id": "PMID:475505", "title": "[Mitral insufficiency after a penetrating thoracic wound. Report of a case].", "content": "It is communicated an isolated mytral insufficiency case, secondary to thorax's penetrative wound by steel arm. There are commented the clinic discoveries, phonomechanocardiographic, echocardiographic, haemodynamic and operation results of a unique orifice in the mytral's septal valve with important overflow. When perforation was sutured it was necessary reoperate it in order to suture's dehiscence and valve's substitution by a Bjork-Shilley's prothesis. The patient persists asymptomatic 35 months after intervention.", "contents": "[Mitral insufficiency after a penetrating thoracic wound. Report of a case]. It is communicated an isolated mytral insufficiency case, secondary to thorax's penetrative wound by steel arm. There are commented the clinic discoveries, phonomechanocardiographic, echocardiographic, haemodynamic and operation results of a unique orifice in the mytral's septal valve with important overflow. When perforation was sutured it was necessary reoperate it in order to suture's dehiscence and valve's substitution by a Bjork-Shilley's prothesis. The patient persists asymptomatic 35 months after intervention."}
{"id": "PMID:475506", "title": "[Arterosclerosis and its prevention. The problem and its natural controversy].", "content": "It scarcely has a glimpse about the knowledge of aterosclerosis nature. About preventive recommendations, it has not yet surpassed hypothetic level, but problem is in force and requires to prove hypothetic and theoretical models that have been proposed. Everything that has been said as preventive recommendations has arisen all kind of opinions that constitute the existent controversy. Discussion is composed by the diametrically adverse points of view, in spite of that they are refered to the same data. Positive aspect that can remain is to centralize the knowledge and to comprehend best the problem nature. Controversy is a part of the collective thinking process about the theme. Obviously no one researcher has the complete solution, neither it is a lonely person work. There are exposed at the work some details of the subjacent process implicit into the term of risk factors. In order to comprehend the controversy, it is necessary to take on account the process that has been followed for the concepts formation, by no one manner it can be taken with frivolity and less to under-value it. Most part of researches on the subject agree that infancy and youthfulness are the best ages to make intervene primary prevention. There are revised these concept's bases and it is concluded that it is necessary at our ambient to acquire own experience about the theme, but we have to be very cautious at the best resources' utilization that are to our disposition.", "contents": "[Arterosclerosis and its prevention. The problem and its natural controversy]. It scarcely has a glimpse about the knowledge of aterosclerosis nature. About preventive recommendations, it has not yet surpassed hypothetic level, but problem is in force and requires to prove hypothetic and theoretical models that have been proposed. Everything that has been said as preventive recommendations has arisen all kind of opinions that constitute the existent controversy. Discussion is composed by the diametrically adverse points of view, in spite of that they are refered to the same data. Positive aspect that can remain is to centralize the knowledge and to comprehend best the problem nature. Controversy is a part of the collective thinking process about the theme. Obviously no one researcher has the complete solution, neither it is a lonely person work. There are exposed at the work some details of the subjacent process implicit into the term of risk factors. In order to comprehend the controversy, it is necessary to take on account the process that has been followed for the concepts formation, by no one manner it can be taken with frivolity and less to under-value it. Most part of researches on the subject agree that infancy and youthfulness are the best ages to make intervene primary prevention. There are revised these concept's bases and it is concluded that it is necessary at our ambient to acquire own experience about the theme, but we have to be very cautious at the best resources' utilization that are to our disposition."}
{"id": "PMID:475512", "title": "Actometric effects of intravenous cocaine in rats.", "content": "Intravenous infusions of cocaine, in dosages which have been reported to maintain self-administration behavior, were administered to cannulated rats. Ten identical infusions were administered at 6 min intervals within a session. The activity occuriring in the initial minute following infusions was compared to that produced by saline infusions. Dosages of 200, 400, 800 and 1200 microgram/kg significantly increased activity. However, this effect was not maintained throughout the session. The tenth infusion no longer increased activity as compared to the initial infusion. Therefore these data would not support the hypothesis that cocaine-induced activity was responsible for maintaining cocaine self-administration behavior in this species. Pretreatment with agents which disrupt the synthesis of dopamine and/or norepinephrine failed to antagonize this initial increase in activity. These data would suggest that the activity effect of cocaine is not dependent on newly synthesized pools of the catecholamines.", "contents": "Actometric effects of intravenous cocaine in rats. Intravenous infusions of cocaine, in dosages which have been reported to maintain self-administration behavior, were administered to cannulated rats. Ten identical infusions were administered at 6 min intervals within a session. The activity occuriring in the initial minute following infusions was compared to that produced by saline infusions. Dosages of 200, 400, 800 and 1200 microgram/kg significantly increased activity. However, this effect was not maintained throughout the session. The tenth infusion no longer increased activity as compared to the initial infusion. Therefore these data would not support the hypothesis that cocaine-induced activity was responsible for maintaining cocaine self-administration behavior in this species. Pretreatment with agents which disrupt the synthesis of dopamine and/or norepinephrine failed to antagonize this initial increase in activity. These data would suggest that the activity effect of cocaine is not dependent on newly synthesized pools of the catecholamines."}
{"id": "PMID:475513", "title": "Effect of purine compounds on the vascular responsiveness of bovine coronary and lingual arteries.", "content": "The vascular effects of several purine compounds were evaluated using isolated arteries from bovine heart and tongue. At almost all concentrations tested, adenosine, AMP, ADP, ATP, guanosine, GMP, GDP and inosine produced significant relaxation of the lingual artery. In general, these compounds were much less effective in the coronary artery. Dipyridamole and nitrobenzylthioinosine (NBMPR), compounds which block the cellular uptake of nucleosides, partially prevented the actions of these compounds in the lingual artery but not in the coronary artery. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), a potent inhibitor of adenosine deaminase also altered the relaxant effect of adenosine. These results suggest that at least part of the action of purine compounds on the vascular smooth muscle of the lingual artery is a result of an intracellular effect.", "contents": "Effect of purine compounds on the vascular responsiveness of bovine coronary and lingual arteries. The vascular effects of several purine compounds were evaluated using isolated arteries from bovine heart and tongue. At almost all concentrations tested, adenosine, AMP, ADP, ATP, guanosine, GMP, GDP and inosine produced significant relaxation of the lingual artery. In general, these compounds were much less effective in the coronary artery. Dipyridamole and nitrobenzylthioinosine (NBMPR), compounds which block the cellular uptake of nucleosides, partially prevented the actions of these compounds in the lingual artery but not in the coronary artery. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), a potent inhibitor of adenosine deaminase also altered the relaxant effect of adenosine. These results suggest that at least part of the action of purine compounds on the vascular smooth muscle of the lingual artery is a result of an intracellular effect."}
{"id": "PMID:475514", "title": "Comparison of various vasodilator drugs using lower body negative pressure.", "content": "The present investigations were designed to enable a differentiation in vivo between various types of vasodilator drugs by studying their influence on mean arterial blood pressure (MAP) in anaesthetized rats, simultaneously subjected to lower body negative pressure (LBNP). In anaesthetized rats the application of various degrees of LBNP caused a proportional decrease in MAP, cardiac output and central venous pressure, whereas heart rate remained unchanged. Reflex tachycardia was not observed as a result of general anaesthesia. The autonomic nervous system does not play a part in the physiological events induced by LBNP, since various pretreatments (vagotomy, adrenalectomy, pharmacological sympathectomy) did not change the response to LBNP. It is concluded that the reduction in MAP due to LBNP is a purely mechanical, haemodynamic phenomenon. Upon combined application of drugs and LBNP, vasodilator drugs which mainly dilate veins (sodium nitrite, isosorbide, nitroglycerine) were more active with respect to their hypotensive action at a LBNP of 5 mm than at 3 mm Hg. However, vasodilator drugs with a predominantly arterial site of action (hydralazine, papaverine, phentolamine, diazoxide) were more potent at a LBNP of 3 mm than at 5 mm Hg. This different behavior may be used to discriminate in vivo between various types of vasodilator drugs. The method is accurate, reproduci0le and relatively easy to perform.", "contents": "Comparison of various vasodilator drugs using lower body negative pressure. The present investigations were designed to enable a differentiation in vivo between various types of vasodilator drugs by studying their influence on mean arterial blood pressure (MAP) in anaesthetized rats, simultaneously subjected to lower body negative pressure (LBNP). In anaesthetized rats the application of various degrees of LBNP caused a proportional decrease in MAP, cardiac output and central venous pressure, whereas heart rate remained unchanged. Reflex tachycardia was not observed as a result of general anaesthesia. The autonomic nervous system does not play a part in the physiological events induced by LBNP, since various pretreatments (vagotomy, adrenalectomy, pharmacological sympathectomy) did not change the response to LBNP. It is concluded that the reduction in MAP due to LBNP is a purely mechanical, haemodynamic phenomenon. Upon combined application of drugs and LBNP, vasodilator drugs which mainly dilate veins (sodium nitrite, isosorbide, nitroglycerine) were more active with respect to their hypotensive action at a LBNP of 5 mm than at 3 mm Hg. However, vasodilator drugs with a predominantly arterial site of action (hydralazine, papaverine, phentolamine, diazoxide) were more potent at a LBNP of 3 mm than at 5 mm Hg. This different behavior may be used to discriminate in vivo between various types of vasodilator drugs. The method is accurate, reproduci0le and relatively easy to perform."}
{"id": "PMID:475515", "title": "On the possible role of adenosine in the hypoxia-induced alterations of the electrical and mechanical activity of the atrial myocardium.", "content": "The effects of adenosine on transmembrane potential and myocardial contractility were compared with those of hypoxia in electrically driven left atrial preparations of guinea-pigs. The amplitude and duration of action potential, contractile force and maximum rate of rise of tension development were decreased by both adenosine and hypoxia. Both the adenosine- and hypoxia-induced changes could readily be prevented by aminophylline, a competitive antagonist of adenosine. Serious changes of transmembrane atrial potential due to long-lasting hypoxia could also be reversed by aminophylline. On the basis of the present results it is assumed that in acute myocardial hypoxia the increased tissue level of adenosine might contribute to the functional impairment of the atrial myocardium.", "contents": "On the possible role of adenosine in the hypoxia-induced alterations of the electrical and mechanical activity of the atrial myocardium. The effects of adenosine on transmembrane potential and myocardial contractility were compared with those of hypoxia in electrically driven left atrial preparations of guinea-pigs. The amplitude and duration of action potential, contractile force and maximum rate of rise of tension development were decreased by both adenosine and hypoxia. Both the adenosine- and hypoxia-induced changes could readily be prevented by aminophylline, a competitive antagonist of adenosine. Serious changes of transmembrane atrial potential due to long-lasting hypoxia could also be reversed by aminophylline. On the basis of the present results it is assumed that in acute myocardial hypoxia the increased tissue level of adenosine might contribute to the functional impairment of the atrial myocardium."}
{"id": "PMID:475516", "title": "The effects of cortisol on protein metabolism and on transfer ribonucleic acid methylase activity in rhabdomyosarcoma of rats.", "content": "The effects of cortisol on protein metabolism were examined in rhabdomyosarcoma tissue, experimentally provoked in rats by a single intramuscular injection of cobalt powder into the adductor muscle of the hind limb. In vivo treatment of tumour bearing animals with 1 and 10 mg cortisol resulted in a reduced incorporation of [2-14C] glycine into the tumour proteins. In vitro application of 0.01 to 100 micrograms cortisol on tumour slices equally reduced the amino acid incorporation into the proteins. These inhibitions could not be explained by modifications in protein, RNA, DNA content or by changes in the membrane function. The factors inhibiting the amino acid incorporation into tumour proteins could be located in the 105,000 X g supernatant protein fraction. The tumour transfer RNA methylase activity became markedly inhibited by cortisol treatment.", "contents": "The effects of cortisol on protein metabolism and on transfer ribonucleic acid methylase activity in rhabdomyosarcoma of rats. The effects of cortisol on protein metabolism were examined in rhabdomyosarcoma tissue, experimentally provoked in rats by a single intramuscular injection of cobalt powder into the adductor muscle of the hind limb. In vivo treatment of tumour bearing animals with 1 and 10 mg cortisol resulted in a reduced incorporation of [2-14C] glycine into the tumour proteins. In vitro application of 0.01 to 100 micrograms cortisol on tumour slices equally reduced the amino acid incorporation into the proteins. These inhibitions could not be explained by modifications in protein, RNA, DNA content or by changes in the membrane function. The factors inhibiting the amino acid incorporation into tumour proteins could be located in the 105,000 X g supernatant protein fraction. The tumour transfer RNA methylase activity became markedly inhibited by cortisol treatment."}
{"id": "PMID:475517", "title": "Hemodialysis in the eighth and ninth decades of life.", "content": "In a review of 45 patients who started receiving hemodialysis (HD) after the age of 70 years (mean, 75 years), compared with a control of 70 HD patients (mean age, 42 years), the two-year survival for elderly patients was 42% and 58% for controls. In the elderly group, age did not correlate with survival. Nine elderly patients were over 80 years old and had a two-year survival of 41%. The elderly patients had a significantly lower mean predialysis blood pressure (BP) (142/73 +/- 3/1 mm Hg) than the controls (158/88 +/- 2/1 mm Hg) (P less than .001). Only 13% of the elderly patients received antihypertensive medication, compared with 41% of controls (P less than .01). The BP showed a significant negative correlation with age in both elderly ( r - .41, P less than .01) and control (r = .35, P less than .001) patients. Glomerulonephritis was less common in the elderly (9%) than control (31%) groups, and pyelonephritis was more common (29% vs 16%).", "contents": "Hemodialysis in the eighth and ninth decades of life. In a review of 45 patients who started receiving hemodialysis (HD) after the age of 70 years (mean, 75 years), compared with a control of 70 HD patients (mean age, 42 years), the two-year survival for elderly patients was 42% and 58% for controls. In the elderly group, age did not correlate with survival. Nine elderly patients were over 80 years old and had a two-year survival of 41%. The elderly patients had a significantly lower mean predialysis blood pressure (BP) (142/73 +/- 3/1 mm Hg) than the controls (158/88 +/- 2/1 mm Hg) (P less than .001). Only 13% of the elderly patients received antihypertensive medication, compared with 41% of controls (P less than .01). The BP showed a significant negative correlation with age in both elderly ( r - .41, P less than .01) and control (r = .35, P less than .001) patients. Glomerulonephritis was less common in the elderly (9%) than control (31%) groups, and pyelonephritis was more common (29% vs 16%)."}
{"id": "PMID:475518", "title": "Diabetes insipidus and breast cancer.", "content": "Diabetes insipidus, resulting from metastatic involvement of the neurohypophysial system, is a rare complication of breast cancer. This review examined the clinical features, metastatic pattern, and radiological and postmortem findings of 39 breast cancer patients with this complication. All patients had polyuria and polydipsia, and all had evidence of advanced metastatic breast cancer. A high incidence of meningeal carcinoma carcinomatosis and/or sellar metastases was observed. In view of the anatomical proximity of the posterior pituitary to the dura mater and the sella turcica, our findings suggest that metastases to the neurohypophysis can occur not only as a result of hematogenous dissemination of malignant cells, but also from direct tumor extension and/or invasion from adjacent structures. Although satisfactory symptomatic relief can be obtained with vasopressin tannate, complete resolution of the diabetic insipidus syndrome was evident only in those patients who had achieved control of the underlying breast disease.", "contents": "Diabetes insipidus and breast cancer. Diabetes insipidus, resulting from metastatic involvement of the neurohypophysial system, is a rare complication of breast cancer. This review examined the clinical features, metastatic pattern, and radiological and postmortem findings of 39 breast cancer patients with this complication. All patients had polyuria and polydipsia, and all had evidence of advanced metastatic breast cancer. A high incidence of meningeal carcinoma carcinomatosis and/or sellar metastases was observed. In view of the anatomical proximity of the posterior pituitary to the dura mater and the sella turcica, our findings suggest that metastases to the neurohypophysis can occur not only as a result of hematogenous dissemination of malignant cells, but also from direct tumor extension and/or invasion from adjacent structures. Although satisfactory symptomatic relief can be obtained with vasopressin tannate, complete resolution of the diabetic insipidus syndrome was evident only in those patients who had achieved control of the underlying breast disease."}
{"id": "PMID:475519", "title": "Clinically occult diffuse proliferative lupus nephritis. An age-related phenomenon.", "content": "Thirteen patients with systemic lupus erythematosus (SLE) who had normal results of urinalysis, absence of proteinuria, and normal serum creatinine values underwent renal biopsy. Three of 13 patients had diffuse proliferative glomerulonephritis (group 1). Biopsy specimens showed segmental fibrinoid necrosis, diffuse mesangial hypercellularity, and substantial immunoglobulin deposition. Group 2 comprised those patients whose histologic findings did not portend a poor prognosis. Four had mesangial proliferative glomerulonephritis, three had focal proliferative glomerulonephritis, and three had minimal mesangial widening. The values of inulin clearance in group 1 did not differ significantly from those in group 2. Patients in group 1 had a mean age of 19 years, a value significantly lower than in group 2 (41.8 years). Review of previous reports also supports the thesis that this phenomenon is age related. Our study underscores the importance of renal biopsy in patients with SLE despite the absence of clinical evidence of renal involvement, particularly in patients under 30 years of age.", "contents": "Clinically occult diffuse proliferative lupus nephritis. An age-related phenomenon. Thirteen patients with systemic lupus erythematosus (SLE) who had normal results of urinalysis, absence of proteinuria, and normal serum creatinine values underwent renal biopsy. Three of 13 patients had diffuse proliferative glomerulonephritis (group 1). Biopsy specimens showed segmental fibrinoid necrosis, diffuse mesangial hypercellularity, and substantial immunoglobulin deposition. Group 2 comprised those patients whose histologic findings did not portend a poor prognosis. Four had mesangial proliferative glomerulonephritis, three had focal proliferative glomerulonephritis, and three had minimal mesangial widening. The values of inulin clearance in group 1 did not differ significantly from those in group 2. Patients in group 1 had a mean age of 19 years, a value significantly lower than in group 2 (41.8 years). Review of previous reports also supports the thesis that this phenomenon is age related. Our study underscores the importance of renal biopsy in patients with SLE despite the absence of clinical evidence of renal involvement, particularly in patients under 30 years of age."}
{"id": "PMID:475520", "title": "Serious staphylococcal infections with strains tolerant to bactericidal antibiotics.", "content": "The clinical response in 20 cases of serious staphylococcal infection was compared with the in vitro resistance or \"tolerance\" of the infecting Staphylococcus to killing by antibiotics used in treatment. Cases were divided into two groups: (1) patients who initially received nonbactericidal antibiotics (ten cases), and (2) patients who initially received bactericidal antibiotics with or without nonbactericidal antibiotics. Mortality due to uncontrolled staphylococcal infection was 40% (4/10) in group 1 as compared with no mortality (1/10) in group0) in group 1 as compared with no mortality (0/10) in group 2. The duration of positive cultures after start of therapy in group 1 (mean, 6.1 days) was significantly longer than that in group 2 (mean, 1.3 days). The duration of fever after start of therapy in group 1 was not significantly different when compared with group 2.", "contents": "Serious staphylococcal infections with strains tolerant to bactericidal antibiotics. The clinical response in 20 cases of serious staphylococcal infection was compared with the in vitro resistance or \"tolerance\" of the infecting Staphylococcus to killing by antibiotics used in treatment. Cases were divided into two groups: (1) patients who initially received nonbactericidal antibiotics (ten cases), and (2) patients who initially received bactericidal antibiotics with or without nonbactericidal antibiotics. Mortality due to uncontrolled staphylococcal infection was 40% (4/10) in group 1 as compared with no mortality (1/10) in group0) in group 1 as compared with no mortality (0/10) in group 2. The duration of positive cultures after start of therapy in group 1 (mean, 6.1 days) was significantly longer than that in group 2 (mean, 1.3 days). The duration of fever after start of therapy in group 1 was not significantly different when compared with group 2."}
{"id": "PMID:475522", "title": "Myocardial infarction in pregnancy associated with a coronary artery thrombus.", "content": "The case of a 36-year-old multigravid woman in whom acute myocardial infarction developed at five months post partum is described. Coronary angiography performed 11 days following myocardial infarction demonstrated a thrombus-like lesion in the coronary artery branch supplying the area of myocardial damage with no other evidence of coronary artery disease. This finding suggests that the development of a coronary artery thrombus is part of the mechanism of myocardial infarction in this case where the phenomenon of angiographically normal coronary arteries following myocardial infarction has bee- described. The mechanism leading to the development of a coronary thrombus in pregnancy is obscure, but may be similar to other clotting abnormalities complicating the third trimester and puerperium.", "contents": "Myocardial infarction in pregnancy associated with a coronary artery thrombus. The case of a 36-year-old multigravid woman in whom acute myocardial infarction developed at five months post partum is described. Coronary angiography performed 11 days following myocardial infarction demonstrated a thrombus-like lesion in the coronary artery branch supplying the area of myocardial damage with no other evidence of coronary artery disease. This finding suggests that the development of a coronary artery thrombus is part of the mechanism of myocardial infarction in this case where the phenomenon of angiographically normal coronary arteries following myocardial infarction has bee- described. The mechanism leading to the development of a coronary thrombus in pregnancy is obscure, but may be similar to other clotting abnormalities complicating the third trimester and puerperium."}
{"id": "PMID:475523", "title": "Severe anemia. Clinical observations in 100 patients with very low hemoglobin levels.", "content": "One hundred consecutive patients with hemoglobin concentration less than 3.5 g/dL (hematocrit reading, less than 10%) were admitted to the University of Baghdad Teaching Hospital, Iraq, during a 30-month period. Twenty-eight patients had aplastic anemia, 27 had leukemia or other hemopoietic malignancies, 16 had chronic renal failure, eight had iron-deficiency anemia, eight had hemolytic anemia, seven had thalassemia major, and six had other conditions. Twenty-three patients died within seven days of admission, mostly due to the underlying disease or complications thereof. Heart failure developed in ten patients, and five had retinal exudates and hemorrhages attributed to severe anemia. Arrhythmias and ECG abnormalities were noted in 20 of 68 patients. Blood transfusion was instituted in all but three patients, whose anemia was corrected with specific therapy without blood transfusion. The tolerance of the 100 patients to such severe anemia was remarkable.", "contents": "Severe anemia. Clinical observations in 100 patients with very low hemoglobin levels. One hundred consecutive patients with hemoglobin concentration less than 3.5 g/dL (hematocrit reading, less than 10%) were admitted to the University of Baghdad Teaching Hospital, Iraq, during a 30-month period. Twenty-eight patients had aplastic anemia, 27 had leukemia or other hemopoietic malignancies, 16 had chronic renal failure, eight had iron-deficiency anemia, eight had hemolytic anemia, seven had thalassemia major, and six had other conditions. Twenty-three patients died within seven days of admission, mostly due to the underlying disease or complications thereof. Heart failure developed in ten patients, and five had retinal exudates and hemorrhages attributed to severe anemia. Arrhythmias and ECG abnormalities were noted in 20 of 68 patients. Blood transfusion was instituted in all but three patients, whose anemia was corrected with specific therapy without blood transfusion. The tolerance of the 100 patients to such severe anemia was remarkable."}
{"id": "PMID:475524", "title": "Inferior vena cava obstruction. A complication of prostate cancer.", "content": "Inferior vena cava (IVC) obstruction, manifested as bilateral, asymmetric, asymptomatic, pitting leg edema and scrotal swelling, developed in two patients with advanced prostatic cancer. Radiological confirmation was obtained in both patients. Inferior vena cava obstruction was the initial manifestation of disease progression and occurred in patients who were ambulatory without evidence of congestive heart failure or concurrent estrogen therapy. Early IVC contrast study is indicated in similar patients in whom asymptomatic bilateral leg edema of obscure origin develops.", "contents": "Inferior vena cava obstruction. A complication of prostate cancer. Inferior vena cava (IVC) obstruction, manifested as bilateral, asymmetric, asymptomatic, pitting leg edema and scrotal swelling, developed in two patients with advanced prostatic cancer. Radiological confirmation was obtained in both patients. Inferior vena cava obstruction was the initial manifestation of disease progression and occurred in patients who were ambulatory without evidence of congestive heart failure or concurrent estrogen therapy. Early IVC contrast study is indicated in similar patients in whom asymptomatic bilateral leg edema of obscure origin develops."}
{"id": "PMID:475525", "title": "Upper extremity lymphedema in filariasis.", "content": "Arm lymphedema associated with shoulder pain and axillary lymphadenopathy was diagnosed in a 40-year-old California man. This is an unusual domestic presentation of filariasis, an endemic tropical disease that is often asymptomatic and undiagnosed. Prognosis for recovery is excellent with the diethylcarbamazine citrate treatment indicated for hemoparasitemia.", "contents": "Upper extremity lymphedema in filariasis. Arm lymphedema associated with shoulder pain and axillary lymphadenopathy was diagnosed in a 40-year-old California man. This is an unusual domestic presentation of filariasis, an endemic tropical disease that is often asymptomatic and undiagnosed. Prognosis for recovery is excellent with the diethylcarbamazine citrate treatment indicated for hemoparasitemia."}
{"id": "PMID:475535", "title": "How big is the normal liver?", "content": "Ultrasonographic determinations of the craniocaudal dimension of the liver in the right midcalvicular line were performed on 96 healthy subjects. These values were compared to those obtained in the same individuals by two techniques of percussion, as well as the values for hepatic dullness predicted for those individuals using a popular nomogram. It was found that the traditional nomogram values, and both techniques of percussion, tended to underestimate this dimension of the liver as determined by ultrasound.", "contents": "How big is the normal liver? Ultrasonographic determinations of the craniocaudal dimension of the liver in the right midcalvicular line were performed on 96 healthy subjects. These values were compared to those obtained in the same individuals by two techniques of percussion, as well as the values for hepatic dullness predicted for those individuals using a popular nomogram. It was found that the traditional nomogram values, and both techniques of percussion, tended to underestimate this dimension of the liver as determined by ultrasound."}
{"id": "PMID:475536", "title": "Adult bacteremic Haemophilus parainfluenzae infections. Seven reports of cases and a review of the literature.", "content": "Seven cases of adult Haemophilus parainfluenzae infections diagnosed by positive blood cultures are compared with cases previously reported in the English literature. Three patients had pneumonia, while the others had epiglottitis with meningitis, pharyngitis, arthritis, and endocarditis, respectively. Nonendocarditic manifestations of adult H parainfluenzae infection were reported in four other cases. In addition to the diseases of our patients, H parainfluenzae also has been isolated from cerebral abscesses. Patients did well with antibiotic therapy and there were no deaths. Patients did well with antibiotic therapy and there were no deaths. Report of antibiotic sensitivity testing of 50 strains disclosed 6% of isolates resistant to ampicillin sodium, with all sensitive to chloramphenicol. If the antibiotic sensitivity of the organism is unknown, then chloramphenicol therapy should be instituted until adequate susceptibility studies have been performed. If the organism is sensitive to ampicillin, then this is the drug of choice.", "contents": "Adult bacteremic Haemophilus parainfluenzae infections. Seven reports of cases and a review of the literature. Seven cases of adult Haemophilus parainfluenzae infections diagnosed by positive blood cultures are compared with cases previously reported in the English literature. Three patients had pneumonia, while the others had epiglottitis with meningitis, pharyngitis, arthritis, and endocarditis, respectively. Nonendocarditic manifestations of adult H parainfluenzae infection were reported in four other cases. In addition to the diseases of our patients, H parainfluenzae also has been isolated from cerebral abscesses. Patients did well with antibiotic therapy and there were no deaths. Patients did well with antibiotic therapy and there were no deaths. Report of antibiotic sensitivity testing of 50 strains disclosed 6% of isolates resistant to ampicillin sodium, with all sensitive to chloramphenicol. If the antibiotic sensitivity of the organism is unknown, then chloramphenicol therapy should be instituted until adequate susceptibility studies have been performed. If the organism is sensitive to ampicillin, then this is the drug of choice."}
{"id": "PMID:475537", "title": "Results of parathyroidectomy for autonomous hyperparathyroidism.", "content": "Autonomous hyperparathyroidism occurred in 15% of 152 patients maintained by long-term home dialysis during the past nine years. Twenty-two patients with elevated serum parathormone levels and progressive bone disease in the presence of normal serum phosphate and calcium levels were treated by subtotal parathyroidectomy. All had parathyroid hyperplasia. Eighteen of the 22 patients are presently alive and undergo dialysis. Symptoms of bone pain, pruritus, and muscle cramps had improved in three fourths of the patients. The serum parathormone level decreased from a preoperative average of 576 muLEq/mL to an average of 188 muLEq/mL postoperatively. All 18 patients, observed for six to 77 months, showed improvement in x-ray films of their bone disease. The autonomous hyperparathyroidism of end-stage renal disease is corrected by subtotal parathyroidectomy, and the effect is sustained.", "contents": "Results of parathyroidectomy for autonomous hyperparathyroidism. Autonomous hyperparathyroidism occurred in 15% of 152 patients maintained by long-term home dialysis during the past nine years. Twenty-two patients with elevated serum parathormone levels and progressive bone disease in the presence of normal serum phosphate and calcium levels were treated by subtotal parathyroidectomy. All had parathyroid hyperplasia. Eighteen of the 22 patients are presently alive and undergo dialysis. Symptoms of bone pain, pruritus, and muscle cramps had improved in three fourths of the patients. The serum parathormone level decreased from a preoperative average of 576 muLEq/mL to an average of 188 muLEq/mL postoperatively. All 18 patients, observed for six to 77 months, showed improvement in x-ray films of their bone disease. The autonomous hyperparathyroidism of end-stage renal disease is corrected by subtotal parathyroidectomy, and the effect is sustained."}
{"id": "PMID:475538", "title": "Circulating levels of vasoactive intestinal polypeptide in liver disease.", "content": "In animals, the effects of vasoactive intestinal polypeptide (VIP) include peripheral vasodilation, hyperdynamic circulation, hyperglycemia, and hyperventilation. Because these phenomena are noted in patients with cirrhosis, it has been postulated that VIP might be escaping hepatic inactivation and entering the systemic circulatory system and contributing to these abnormalities. The major purpose of this study is to establish whether or not VIP levels are elevated in patients with cirrhosis. Additional goals are to determine if VIP levels are elevated in acute liver disease and in chronic illnesses with secondary liver involvement. The data demonstrate that patients with cirrhosis and those with acute liver disease or chronic illnesses with secondary hepatic involvement have a wide range of VIP levels with mean values significantly above that of normal individuals and patients with chronic illness and no liver involvement.", "contents": "Circulating levels of vasoactive intestinal polypeptide in liver disease. In animals, the effects of vasoactive intestinal polypeptide (VIP) include peripheral vasodilation, hyperdynamic circulation, hyperglycemia, and hyperventilation. Because these phenomena are noted in patients with cirrhosis, it has been postulated that VIP might be escaping hepatic inactivation and entering the systemic circulatory system and contributing to these abnormalities. The major purpose of this study is to establish whether or not VIP levels are elevated in patients with cirrhosis. Additional goals are to determine if VIP levels are elevated in acute liver disease and in chronic illnesses with secondary liver involvement. The data demonstrate that patients with cirrhosis and those with acute liver disease or chronic illnesses with secondary hepatic involvement have a wide range of VIP levels with mean values significantly above that of normal individuals and patients with chronic illness and no liver involvement."}
{"id": "PMID:475539", "title": "Effect of hemodialysis on glucose tolerance in children with chronic renal failure.", "content": "Five uremic (7.0 - 15.2 years), non-dialyzed children treated medically (group 1) had an intravenous glucose tolerance test (0.5 g/kg). Blood samples were obtained at frequent intervals and duplicate determinations of plasma glucose (glucose-oxidase method on Somogyi-Nelson protein-free filtrates), insulin and free fatty acids were performed. The same study was performed in eleven children (12.3 - 18.0 years) with chronic renal failure undergoing long-term hemodialysis (group 2), before and two hours after dialysis. Results were compared to a group (group 3) of eight healthy children (7.5 -- 17.5 years). In group 1 a \"normal\" insulin response was observed in the presence of hyperglycemia and the plasma glucose clearance was in the diabetic range. Twice weekly hemodialysis produced no beneficial acute effects on group 2, based on absolute glucose values or plasma glucose clearance rates, on groups as well as on individual basis; however, a clear hyperinsulinemia was present which was of even higher magnitude three and six months after the initial study. These results demonstrated that chronic hemodialysis had no beneficial acute effects on plasma glucose concentration or plasma glucose clearance rate. However, it may have obvious long term beneficial effects on glucose tolerance by enhancing pancreatic insulin release (to overcome peripheral insulin resistance) to such an extent as to maintain a normal carbohydrate tolerance.", "contents": "Effect of hemodialysis on glucose tolerance in children with chronic renal failure. Five uremic (7.0 - 15.2 years), non-dialyzed children treated medically (group 1) had an intravenous glucose tolerance test (0.5 g/kg). Blood samples were obtained at frequent intervals and duplicate determinations of plasma glucose (glucose-oxidase method on Somogyi-Nelson protein-free filtrates), insulin and free fatty acids were performed. The same study was performed in eleven children (12.3 - 18.0 years) with chronic renal failure undergoing long-term hemodialysis (group 2), before and two hours after dialysis. Results were compared to a group (group 3) of eight healthy children (7.5 -- 17.5 years). In group 1 a \"normal\" insulin response was observed in the presence of hyperglycemia and the plasma glucose clearance was in the diabetic range. Twice weekly hemodialysis produced no beneficial acute effects on group 2, based on absolute glucose values or plasma glucose clearance rates, on groups as well as on individual basis; however, a clear hyperinsulinemia was present which was of even higher magnitude three and six months after the initial study. These results demonstrated that chronic hemodialysis had no beneficial acute effects on plasma glucose concentration or plasma glucose clearance rate. However, it may have obvious long term beneficial effects on glucose tolerance by enhancing pancreatic insulin release (to overcome peripheral insulin resistance) to such an extent as to maintain a normal carbohydrate tolerance."}
{"id": "PMID:475540", "title": "Acute autonomic neuropathy.", "content": "Case report of acute autonomic neuropathy. It is the twelfth case reported in literature and the second case associated to positive serological tests for infectious mononucleosis. We are reporting for the first time plasma renin activity, decrease response to repetitive pacing of the S-A node, insulin curve with radio-immunoassay and cardiac catheterization findings.", "contents": "Acute autonomic neuropathy. Case report of acute autonomic neuropathy. It is the twelfth case reported in literature and the second case associated to positive serological tests for infectious mononucleosis. We are reporting for the first time plasma renin activity, decrease response to repetitive pacing of the S-A node, insulin curve with radio-immunoassay and cardiac catheterization findings."}
{"id": "PMID:475541", "title": "The form of early development. Continuity and discontinuity in emergent competences.", "content": "Conceptions of psychological development devised by Western theorists have assumed connectivity of stages, stability of acquired structures, and continuity of process, and have resisted the possibility that some emerging psychological qualities might have relatively short histories. Empirical studies of the first two years of life suggest that the enhancement of retrieval memory at 8 to 10 months of age, the emergence of a linguistic set at 15 to 18 months of age, and sensitivity to standards of competence prior to the second birthday are emergent human competences that may not necessarily have a historical link to the events of early infancy. Developmental theorists should be as receptive to sequences that are relatively discontinuous as they are to those that are gradual and cumulative.", "contents": "The form of early development. Continuity and discontinuity in emergent competences. Conceptions of psychological development devised by Western theorists have assumed connectivity of stages, stability of acquired structures, and continuity of process, and have resisted the possibility that some emerging psychological qualities might have relatively short histories. Empirical studies of the first two years of life suggest that the enhancement of retrieval memory at 8 to 10 months of age, the emergence of a linguistic set at 15 to 18 months of age, and sensitivity to standards of competence prior to the second birthday are emergent human competences that may not necessarily have a historical link to the events of early infancy. Developmental theorists should be as receptive to sequences that are relatively discontinuous as they are to those that are gradual and cumulative."}
{"id": "PMID:475542", "title": "A comparative trial of home and hospital psychiatric care. One-year follow-up.", "content": "The effectiveness of community-based treatment stressing home care was compared with hospital-based psychiatric care. One hundred and fifty-five patients destined for inpatient psychiatric care were randomly assigned to Home Care (76 patients) and to Hospital Care (79 patients). Symptoms, role functioning, and psychosocial burden on the family were similar at admission, one month, three months, six months, and one year. The mean in-hospital stay of Hospital Care patients was 41.7 days compared with a mean stay of 14.5 days for Home Care patients. The difference in the amount of ambulatory care received by patients in the two groups was not significant. The evidence is consistent: community-based psychiatric care is an effective alternative to hospital-based care for many but not all severely disabled patients. The active ingredients of successful community treatment are known, yet the lag in implementing these programs persists.", "contents": "A comparative trial of home and hospital psychiatric care. One-year follow-up. The effectiveness of community-based treatment stressing home care was compared with hospital-based psychiatric care. One hundred and fifty-five patients destined for inpatient psychiatric care were randomly assigned to Home Care (76 patients) and to Hospital Care (79 patients). Symptoms, role functioning, and psychosocial burden on the family were similar at admission, one month, three months, six months, and one year. The mean in-hospital stay of Hospital Care patients was 41.7 days compared with a mean stay of 14.5 days for Home Care patients. The difference in the amount of ambulatory care received by patients in the two groups was not significant. The evidence is consistent: community-based psychiatric care is an effective alternative to hospital-based care for many but not all severely disabled patients. The active ingredients of successful community treatment are known, yet the lag in implementing these programs persists."}
{"id": "PMID:475543", "title": "Novel antidepressants and the biogenic amine hypothesis of depression. The case for iprindole and mianserin.", "content": "The introduction of two tricyclic compounds (iprindole and mianserin) that are reported to have antidepressant properties but to be relatively devoid of effects on central amine neurotransmitter systems has raised questions about the amine hypothesis of depression and about the mechanism of action of tricyclics in general. In view of the importance of these questions, a critical review of both the clinical and pharmacological profiles of iprindole and mianserin was undertaken. Iprindole is a relatively weak inhibitor of both norepinephrine (NE) and serotonin, whereas mianserin possesses at least modest potency as an inhibitor of NE uptake. However, the evidence is as yet insufficient to prove the superiority of iprindole over placebo in the treatment of those depressions characterized by endogenous symptoms. In considering the pharmacological profiles of these two drugs together with their clinical profiles, the data are not inconsistent with the hypothesized role of biogenic amines in major depression.", "contents": "Novel antidepressants and the biogenic amine hypothesis of depression. The case for iprindole and mianserin. The introduction of two tricyclic compounds (iprindole and mianserin) that are reported to have antidepressant properties but to be relatively devoid of effects on central amine neurotransmitter systems has raised questions about the amine hypothesis of depression and about the mechanism of action of tricyclics in general. In view of the importance of these questions, a critical review of both the clinical and pharmacological profiles of iprindole and mianserin was undertaken. Iprindole is a relatively weak inhibitor of both norepinephrine (NE) and serotonin, whereas mianserin possesses at least modest potency as an inhibitor of NE uptake. However, the evidence is as yet insufficient to prove the superiority of iprindole over placebo in the treatment of those depressions characterized by endogenous symptoms. In considering the pharmacological profiles of these two drugs together with their clinical profiles, the data are not inconsistent with the hypothesized role of biogenic amines in major depression."}
{"id": "PMID:475544", "title": "Urinary MHPG levels and tricyclic antidepressant drug selection. A preliminary communication on improved drug selection in clinical practice.", "content": "The 24-hour urinary 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) output was used as the basis for selection of tricyclic antidepressant drug therapy for the depressed patient population treated by one psychiatrist over a period of ten months in a psychiatric clinic. Use of MHPG output level as the criterion for drug selection resulted in significantly better clinical results than had been obtained previously by the same psychiatrist using more traditional selection methods on a similar depressed patient population. A correlation was noted between patients' pretreatment MHPG output levels and three symptoms of depression (guilt, agitation, and diurnal variation) as measured on the Hamilton Rating Scale for Depression.", "contents": "Urinary MHPG levels and tricyclic antidepressant drug selection. A preliminary communication on improved drug selection in clinical practice. The 24-hour urinary 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) output was used as the basis for selection of tricyclic antidepressant drug therapy for the depressed patient population treated by one psychiatrist over a period of ten months in a psychiatric clinic. Use of MHPG output level as the criterion for drug selection resulted in significantly better clinical results than had been obtained previously by the same psychiatrist using more traditional selection methods on a similar depressed patient population. A correlation was noted between patients' pretreatment MHPG output levels and three symptoms of depression (guilt, agitation, and diurnal variation) as measured on the Hamilton Rating Scale for Depression."}
{"id": "PMID:475545", "title": "Suicide in Britain. More attempts, fewer deaths, lessons for public policy.", "content": "The steady reduction in suicide deaths in Britain from 1963 to 1971 might superficially be credited to systematic programs to reduce suicidal behavior; but the actual saving of lives does not reflect the real upward trend in total suicidal behavior. The number of would-be suicides was in fact increasing steadily, while the proportion of fatal outcomes was being reduced, probably because the methods most commonly used for suicide had become less lethal and methods of resuscitation had improved. Attempts to reduce the amount of suicidal behavior by psychiatric treatment and by suicide prevention programs (primary prevention) seem therefore to have had little measurable impact so far. Although reduction in deaths (secondary prevention) is a more limited goal, the evidence presented justifies more systematic and deliberate efforts to reduce the lethality of the methods available.", "contents": "Suicide in Britain. More attempts, fewer deaths, lessons for public policy. The steady reduction in suicide deaths in Britain from 1963 to 1971 might superficially be credited to systematic programs to reduce suicidal behavior; but the actual saving of lives does not reflect the real upward trend in total suicidal behavior. The number of would-be suicides was in fact increasing steadily, while the proportion of fatal outcomes was being reduced, probably because the methods most commonly used for suicide had become less lethal and methods of resuscitation had improved. Attempts to reduce the amount of suicidal behavior by psychiatric treatment and by suicide prevention programs (primary prevention) seem therefore to have had little measurable impact so far. Although reduction in deaths (secondary prevention) is a more limited goal, the evidence presented justifies more systematic and deliberate efforts to reduce the lethality of the methods available."}
{"id": "PMID:475546", "title": "Specific vs nonspecific factors in psychotherapy. A controlled study of outcome.", "content": "This study explored the relative contribution of the therapist's technical skills and the qualities inherent in any good human relationship to outcome in time-limited individual psychotherapy. Highly experienced psychotherapists treated 15 patients drawn from a relatively homogeneous patient population (male college students, selected primarily on the basis of elevations on the depression, anxiety, and social introversion scales of the Minnesota Multiphasic Personality Inventory). By traditional diagnostic categories, they would be classified as neurotic depression or anxiety reactions. Obsessional trends and borderline personalities were common. A comparable patient group was treated by college professors chosen for their ability to form understanding relationships. Patients treated by professors showed, on the average, as much improvement as patients treated by professional therapists. Treated groups slightly exceeded the controls. Group means, however, obscured considerable individual variability.", "contents": "Specific vs nonspecific factors in psychotherapy. A controlled study of outcome. This study explored the relative contribution of the therapist's technical skills and the qualities inherent in any good human relationship to outcome in time-limited individual psychotherapy. Highly experienced psychotherapists treated 15 patients drawn from a relatively homogeneous patient population (male college students, selected primarily on the basis of elevations on the depression, anxiety, and social introversion scales of the Minnesota Multiphasic Personality Inventory). By traditional diagnostic categories, they would be classified as neurotic depression or anxiety reactions. Obsessional trends and borderline personalities were common. A comparable patient group was treated by college professors chosen for their ability to form understanding relationships. Patients treated by professors showed, on the average, as much improvement as patients treated by professional therapists. Treated groups slightly exceeded the controls. Group means, however, obscured considerable individual variability."}
{"id": "PMID:475547", "title": "Significance of serum fucose, sialic acid, haptoglobine and phospholipids levels in the evolution and treatment of breast cancer.", "content": "Serum fucose, sialic acid, haptoglobine and phospholipids were determined in 167 women with breast cancer stages I--III, 30 with benign lesions of the breast, 42 women in various physiological states of the mammary gland (pregnancy, early childbed and lactation) and compared with 30 healthy women as control. Serial determinations of these parameters during the radio-surgical treatment were done in 28 patients with breast cancer stage III. Fucose and phospholipids levels were significantly increased respectively decreased in the group of patients with breast cancers but unmodified in the others. Sialic acid and haptoglobine -- increased in patients with cancer -- were also elevated in patients with early childbed and benign affections of the breast. The surveillance of these four parameters during the radio-surgical treatment of breast cancer evidenced a good correlation between their modified levels and clinical state of the patients. The increase in fucose, sialic acid and haptoglobine respectively the decrease in phospholipids levels was associated with the clinical evidence of recurrences and metastases.", "contents": "Significance of serum fucose, sialic acid, haptoglobine and phospholipids levels in the evolution and treatment of breast cancer. Serum fucose, sialic acid, haptoglobine and phospholipids were determined in 167 women with breast cancer stages I--III, 30 with benign lesions of the breast, 42 women in various physiological states of the mammary gland (pregnancy, early childbed and lactation) and compared with 30 healthy women as control. Serial determinations of these parameters during the radio-surgical treatment were done in 28 patients with breast cancer stage III. Fucose and phospholipids levels were significantly increased respectively decreased in the group of patients with breast cancers but unmodified in the others. Sialic acid and haptoglobine -- increased in patients with cancer -- were also elevated in patients with early childbed and benign affections of the breast. The surveillance of these four parameters during the radio-surgical treatment of breast cancer evidenced a good correlation between their modified levels and clinical state of the patients. The increase in fucose, sialic acid and haptoglobine respectively the decrease in phospholipids levels was associated with the clinical evidence of recurrences and metastases."}
{"id": "PMID:475548", "title": "[Interference microscopic determinations of dry mass of cell nuclei of normal and dysplastic epithelium, carcinoma in situ and cervical carcinoma cultivated in vitro (author's transl)].", "content": "Dry mass of cell nuclei was studied in 34 explant cultures of normal epithelium (8), persisting dysplasias (7), Ca in situ (9) and cervical carcinomas (10) by interference microscopic determination. Dysplasias, Ca in situ and carcinomas can be distinguished from normal epithelium by a significantly higher dry mass value. Such a difference does not exist between dysplasia and Ca in situ. The percentage of dispersion of the dry mass is significantly higher in carcinomas than in all other examined tissues. The biological meaning of these results is discussed. The interference microscopic determination of dry mass of the cell nuclei is a suitable parameter for the characterization of properties of cervical carcinomas and their intraepithelial prestages cultivated in vitro.", "contents": "[Interference microscopic determinations of dry mass of cell nuclei of normal and dysplastic epithelium, carcinoma in situ and cervical carcinoma cultivated in vitro (author's transl)]. Dry mass of cell nuclei was studied in 34 explant cultures of normal epithelium (8), persisting dysplasias (7), Ca in situ (9) and cervical carcinomas (10) by interference microscopic determination. Dysplasias, Ca in situ and carcinomas can be distinguished from normal epithelium by a significantly higher dry mass value. Such a difference does not exist between dysplasia and Ca in situ. The percentage of dispersion of the dry mass is significantly higher in carcinomas than in all other examined tissues. The biological meaning of these results is discussed. The interference microscopic determination of dry mass of the cell nuclei is a suitable parameter for the characterization of properties of cervical carcinomas and their intraepithelial prestages cultivated in vitro."}
{"id": "PMID:475549", "title": "[On the problem of the \"more early\" diagnosis of spinal cord tumours in children and infants (author's transl)].", "content": "A report is given on 40 spinal cord tumours in children and infants, the diagnosis of which was considerably delayed in part. 28 children were hospitalised because of fixed paralysis, incomplete or complete compression of spinal cord or cauda. Persisting pain in various sections of the spine, often combined with abnormalities in posture and position are suspicious on tumour as long as another cause is proved. X-ray pictures and analysis of lumbarcerebrospinal fluid are in the most cases of decisive importance for a progressing diagnosis. Myeloscintigraphy is specially useful for an early and exclusing diagnosis.", "contents": "[On the problem of the \"more early\" diagnosis of spinal cord tumours in children and infants (author's transl)]. A report is given on 40 spinal cord tumours in children and infants, the diagnosis of which was considerably delayed in part. 28 children were hospitalised because of fixed paralysis, incomplete or complete compression of spinal cord or cauda. Persisting pain in various sections of the spine, often combined with abnormalities in posture and position are suspicious on tumour as long as another cause is proved. X-ray pictures and analysis of lumbarcerebrospinal fluid are in the most cases of decisive importance for a progressing diagnosis. Myeloscintigraphy is specially useful for an early and exclusing diagnosis."}
{"id": "PMID:475550", "title": "[Clinical administration of BCG-immunotherapy in malignant melanoma (author's transl)].", "content": "In the Department of Dermatology of the University of Jena 60 patients with malignant melanoma received the immunotherapy by means of the BCG high-dosis scarification method after maximum surgical removal of the tumour. The results after 3 years of treatment demonstrate that the BCG strain Jena can cause immunostimulation. Rates of remission and survival could be well influenced. No serious side effects were observed.", "contents": "[Clinical administration of BCG-immunotherapy in malignant melanoma (author's transl)]. In the Department of Dermatology of the University of Jena 60 patients with malignant melanoma received the immunotherapy by means of the BCG high-dosis scarification method after maximum surgical removal of the tumour. The results after 3 years of treatment demonstrate that the BCG strain Jena can cause immunostimulation. Rates of remission and survival could be well influenced. No serious side effects were observed."}
{"id": "PMID:475551", "title": "[Hyaluronic acid in pleural fluids: an additional parameter for clinical diagnosis on diffuse mesotheliomas (author's transl)].", "content": "187 pleural fluids of various etiology are examined with the intention to find hyaluronic acid. The colorimetric method of Morgan-Elson was used for this purpose. The mucopolysaccharid was identified in 34 cases: 18 in 30 cases of diffuse mesotheliomas 1 in 74 cases of secondary tumours of the pleura 8 in 20 cases of tuberculous pleurisy 5 in 37 cases of nonspecific pleurisy 2 in 6 cases of purulent pleursy. A level from more than 212 mg/litre was detected in 10 cases of mesotheliomas only. Such a concentration is consequently the proof for diffuse mesothelioma. The diagnostical improvement in other cases of mesotheliomas resulted from a combination of the following parameters: coagulation of protein by the method of Weltmann, cytological investigation of cellsedimentation and measurement of hyaluronic acid. Our experience with the diagnosis of diffuse mesotheliomas is discussed also in account to the results which are published since 1965 from a scientific team working in Lille, France, reporting the value of detection and measurement on hyaluronic acid in pleural fluids.", "contents": "[Hyaluronic acid in pleural fluids: an additional parameter for clinical diagnosis on diffuse mesotheliomas (author's transl)]. 187 pleural fluids of various etiology are examined with the intention to find hyaluronic acid. The colorimetric method of Morgan-Elson was used for this purpose. The mucopolysaccharid was identified in 34 cases: 18 in 30 cases of diffuse mesotheliomas 1 in 74 cases of secondary tumours of the pleura 8 in 20 cases of tuberculous pleurisy 5 in 37 cases of nonspecific pleurisy 2 in 6 cases of purulent pleursy. A level from more than 212 mg/litre was detected in 10 cases of mesotheliomas only. Such a concentration is consequently the proof for diffuse mesothelioma. The diagnostical improvement in other cases of mesotheliomas resulted from a combination of the following parameters: coagulation of protein by the method of Weltmann, cytological investigation of cellsedimentation and measurement of hyaluronic acid. Our experience with the diagnosis of diffuse mesotheliomas is discussed also in account to the results which are published since 1965 from a scientific team working in Lille, France, reporting the value of detection and measurement on hyaluronic acid in pleural fluids."}
{"id": "PMID:475552", "title": "[Endocarditis in cancer necropsies (author's transl)].", "content": "Terminal endocarditis develope in cancer patients almost latently. There is no difference between the so-called \"tumor-endocarditis\" and other verrucous endocarditis associated with terminal tuberculosis, sepsis or rheumatism. It is more frequent in cases with large or ulcerated primary tumours and multiple metastases than in cases with early cancer. It also develope more frequently in well differentiated cancer (squamous and adenocarcinoma) than in indifferentiated forms of cancer. Terminal endocarditis is often seen in patients with cancer of the gallbladder, pancreas, liver, stomach, rectum, and ovary. In carcinoma of the liver, pancreas and biliary tract the trend to embolism is more reduced through icterus than the trend to terminal endocarditis.", "contents": "[Endocarditis in cancer necropsies (author's transl)]. Terminal endocarditis develope in cancer patients almost latently. There is no difference between the so-called \"tumor-endocarditis\" and other verrucous endocarditis associated with terminal tuberculosis, sepsis or rheumatism. It is more frequent in cases with large or ulcerated primary tumours and multiple metastases than in cases with early cancer. It also develope more frequently in well differentiated cancer (squamous and adenocarcinoma) than in indifferentiated forms of cancer. Terminal endocarditis is often seen in patients with cancer of the gallbladder, pancreas, liver, stomach, rectum, and ovary. In carcinoma of the liver, pancreas and biliary tract the trend to embolism is more reduced through icterus than the trend to terminal endocarditis."}
{"id": "PMID:475553", "title": "[The lymphocyte transformation test and its relation to the duration of disease in patients with oral carcinomas (author's transl)].", "content": "Cell mediated immune reactions take an increasing part for the progress of malignant tumors besides numerous other factors. With support of the lymphocyte transformation test a significant difference was pointed out between orofacial tumors with long course and a control group of healthy persons by means of the interpretation of the PHA (phythemagglutinin)-stimulation. These results are to find indicated also in comparison of the antigen preparation with candidin in tumors with long course.", "contents": "[The lymphocyte transformation test and its relation to the duration of disease in patients with oral carcinomas (author's transl)]. Cell mediated immune reactions take an increasing part for the progress of malignant tumors besides numerous other factors. With support of the lymphocyte transformation test a significant difference was pointed out between orofacial tumors with long course and a control group of healthy persons by means of the interpretation of the PHA (phythemagglutinin)-stimulation. These results are to find indicated also in comparison of the antigen preparation with candidin in tumors with long course."}
{"id": "PMID:475554", "title": "[Germinating capacity of Clostridium butyricum Jena H 8 in transplantation tumors (author's transl)].", "content": "Following parenteral application of spores of Clostridium butyricum their intratumoral germination and oncolysis were demonstrated on a variety of mouse transplantation tumors. The germination and propagation of the clostridia was restricted exclusively to the tumor tissue, they could not be demonstrated bacterioscopically in parallelly run normal tissue specimens. The observed differences of germination and lysis rate are due, in part, to tumor host and transplantate-specific properties.", "contents": "[Germinating capacity of Clostridium butyricum Jena H 8 in transplantation tumors (author's transl)]. Following parenteral application of spores of Clostridium butyricum their intratumoral germination and oncolysis were demonstrated on a variety of mouse transplantation tumors. The germination and propagation of the clostridia was restricted exclusively to the tumor tissue, they could not be demonstrated bacterioscopically in parallelly run normal tissue specimens. The observed differences of germination and lysis rate are due, in part, to tumor host and transplantate-specific properties."}
{"id": "PMID:475555", "title": "Cytotoxic action of diethanolamine oleate on Ehrlich exudative carcinoma in mice, compared with the action of polyoxyethylene sorbitan mono-oleate (Tween 80).", "content": "The salt of oleic acid and diethanolamine, and polyoxyethylene sorbitan mono-oleate inhibited growth of Ehrlich exudative carcinoma in mice.", "contents": "Cytotoxic action of diethanolamine oleate on Ehrlich exudative carcinoma in mice, compared with the action of polyoxyethylene sorbitan mono-oleate (Tween 80). The salt of oleic acid and diethanolamine, and polyoxyethylene sorbitan mono-oleate inhibited growth of Ehrlich exudative carcinoma in mice."}
{"id": "PMID:475556", "title": "The influence of 6-hydroxydopamine and 6-hydroxydopa on the phospholipids content in the brain of rats.", "content": "6-hydroxydopamine and 6-hydroxydopa injected intraperitoneally in dose of 50 microgram/g in newborn rats, two and six weeks old caused a drop in content of phosphatidylcholine and phosphatidylserine, and an increase in content of phosphatidylinositol in the brains of two weeks old rats treated with 6-hydroxydopamine in comparison with controls. 6-hydroxydopa administration caused an increase of the content of phosphatidylcholine and phosphatidylinositol and decrease of diphosphatidylglycerol in the brain of two weeks old rats. In the brains of six weeks old rats only a decrease in content of phosphatidic acid was observed after administration of either 6-hydroxydopamine or 6-hydroxydopa. The results are further evidence of a nonspecific toxic action of both compounds.", "contents": "The influence of 6-hydroxydopamine and 6-hydroxydopa on the phospholipids content in the brain of rats. 6-hydroxydopamine and 6-hydroxydopa injected intraperitoneally in dose of 50 microgram/g in newborn rats, two and six weeks old caused a drop in content of phosphatidylcholine and phosphatidylserine, and an increase in content of phosphatidylinositol in the brains of two weeks old rats treated with 6-hydroxydopamine in comparison with controls. 6-hydroxydopa administration caused an increase of the content of phosphatidylcholine and phosphatidylinositol and decrease of diphosphatidylglycerol in the brain of two weeks old rats. In the brains of six weeks old rats only a decrease in content of phosphatidic acid was observed after administration of either 6-hydroxydopamine or 6-hydroxydopa. The results are further evidence of a nonspecific toxic action of both compounds."}
{"id": "PMID:475557", "title": "Influence of chlorpromazine, diazepam, imipramine and pyrazole on ethanol-induced changes in activity of some enzymes in isolated rat liver.", "content": "Studies on the isolated rat liver showed distinct interaction of chlorpromazine, diazepam and imipramine with ethanol. Injected intraperitoneally in doses of 20 mg/kg, these drugs distinctly influenced elimination of ethanol, although not as strongly as pyrazole in vitro in the concentration of 20 mg/100 ml. On the other hand, ethanol altered the effect of these substances on the glucose curve, lactate and pyruvate levels, and activities of glutamic pyruvic and oxalacetic transaminases and aldolase.", "contents": "Influence of chlorpromazine, diazepam, imipramine and pyrazole on ethanol-induced changes in activity of some enzymes in isolated rat liver. Studies on the isolated rat liver showed distinct interaction of chlorpromazine, diazepam and imipramine with ethanol. Injected intraperitoneally in doses of 20 mg/kg, these drugs distinctly influenced elimination of ethanol, although not as strongly as pyrazole in vitro in the concentration of 20 mg/100 ml. On the other hand, ethanol altered the effect of these substances on the glucose curve, lactate and pyruvate levels, and activities of glutamic pyruvic and oxalacetic transaminases and aldolase."}
{"id": "PMID:475558", "title": "A temporal profile of changes in myocardial manganese after isoprenaline induced cardiac necrosis in albino rats.", "content": "Myocardial-necrosis was produced in two groups of albino rats by subcutaneous injection of isoprenaline in doses of 85 mg/kg and 42.5 mg/kg. The necrosis was confirmed by ECG changes, increased serum activities of AspAT, AlAT, GPT and LDH, and by histological examination. Myocardial manganese after isoprenaline administration was estimated over a period of 5 days. The decrease in myocardial manganese was proportional to the extent of cardiac damage. The possible mechanisms and the importance of these changes are discussed.", "contents": "A temporal profile of changes in myocardial manganese after isoprenaline induced cardiac necrosis in albino rats. Myocardial-necrosis was produced in two groups of albino rats by subcutaneous injection of isoprenaline in doses of 85 mg/kg and 42.5 mg/kg. The necrosis was confirmed by ECG changes, increased serum activities of AspAT, AlAT, GPT and LDH, and by histological examination. Myocardial manganese after isoprenaline administration was estimated over a period of 5 days. The decrease in myocardial manganese was proportional to the extent of cardiac damage. The possible mechanisms and the importance of these changes are discussed."}
{"id": "PMID:475559", "title": "Central action of new imido derivatives of succinic acid.", "content": "New imido derivatives of succinic acid were screened pharmacologically with regard to their influence on the central nervous system. No relation was found between the character and position of substituents and depressant action on the CNS. However, it was remarked that potentiation of the central influence of DOPA probably depends on the position of the p-chlorophenyl group.", "contents": "Central action of new imido derivatives of succinic acid. New imido derivatives of succinic acid were screened pharmacologically with regard to their influence on the central nervous system. No relation was found between the character and position of substituents and depressant action on the CNS. However, it was remarked that potentiation of the central influence of DOPA probably depends on the position of the p-chlorophenyl group."}
{"id": "PMID:475560", "title": "Central action of new derivatives of tetrahydropirimidinedione-4,6.", "content": "The central action and LD50 of 11 new 2,5-substituted derivates of tetrahydropirimidinedione-4,6 with an aryl group at C2 were investigated. The most favorable action was exerted by 2-furfurylamine derivatives with an alkil or benzyl group at C5. These compounds acted in doses of 0.0025--0.01 of their LD50 synergistically with chloral hydrate and strongly with hexobarbital, delayed convulsions induced with pentetrazole and potentiated the central action of DOPA in mice pargyline-inhibited MAO activity. They did not antagonize electrogenic convulsions, amphetamine potentiated motility and the action of reserpine, and had no analgetic action. Their LD50s were 670-- 1660 mg/kg.", "contents": "Central action of new derivatives of tetrahydropirimidinedione-4,6. The central action and LD50 of 11 new 2,5-substituted derivates of tetrahydropirimidinedione-4,6 with an aryl group at C2 were investigated. The most favorable action was exerted by 2-furfurylamine derivatives with an alkil or benzyl group at C5. These compounds acted in doses of 0.0025--0.01 of their LD50 synergistically with chloral hydrate and strongly with hexobarbital, delayed convulsions induced with pentetrazole and potentiated the central action of DOPA in mice pargyline-inhibited MAO activity. They did not antagonize electrogenic convulsions, amphetamine potentiated motility and the action of reserpine, and had no analgetic action. Their LD50s were 670-- 1660 mg/kg."}
{"id": "PMID:475563", "title": "Acetabular reinforcement in total hip replacement.", "content": "In conclusion, based on our experimental and clinical material and analysis of failures, we recommend wire mesh reinforcement and bone graft for patients with mild to moderate protrusio. If the floor is strong but the pillars deficient, then the Eichler ring alone is the best implant. The combined system of wire mesh and Eichler ring should be used in cases of protrusion with a large deficiency of the acetabular floor and for protrusio with pillar weakness. Furthermore a bone graft should be used to reinforce the acetabular floor in addition to any prosthetic device.", "contents": "Acetabular reinforcement in total hip replacement. In conclusion, based on our experimental and clinical material and analysis of failures, we recommend wire mesh reinforcement and bone graft for patients with mild to moderate protrusio. If the floor is strong but the pillars deficient, then the Eichler ring alone is the best implant. The combined system of wire mesh and Eichler ring should be used in cases of protrusion with a large deficiency of the acetabular floor and for protrusio with pillar weakness. Furthermore a bone graft should be used to reinforce the acetabular floor in addition to any prosthetic device."}
{"id": "PMID:475564", "title": "[Differential diagnosis of a metastasizing adamantinoma of the tibia and fibula (author's transl)].", "content": "A case of an adamantinoma of the tibia and fibula is described in a 10-year-old girl who died after 17 years with pulmonary metastasis. For a long time, an exact diagnosis couldn't be made. Histologically the lesion was called chronic osteomyelitis (Brodie's abscess), synovial sarcoma and spindle cell sarcoma. The revision of the histological sections revealed a fibrous dysplasia-like pattern in the first lesion of the tibia with tiny foci of an epitheloid-like pattern. This had could be the key to the right diagnosis, because sometimes adamantinoma of long bones is associated with a fibrous dysplasia-like pattern. The reason for misinterpretation of the histological features is seen in the typical variable histological pattern of this tumor (basaloids, squamoid, spindled and tubular pattern). In this connection is refered to the necessity to correlate the histological feature with the X-ray in making diagnosis in bone pathology.", "contents": "[Differential diagnosis of a metastasizing adamantinoma of the tibia and fibula (author's transl)]. A case of an adamantinoma of the tibia and fibula is described in a 10-year-old girl who died after 17 years with pulmonary metastasis. For a long time, an exact diagnosis couldn't be made. Histologically the lesion was called chronic osteomyelitis (Brodie's abscess), synovial sarcoma and spindle cell sarcoma. The revision of the histological sections revealed a fibrous dysplasia-like pattern in the first lesion of the tibia with tiny foci of an epitheloid-like pattern. This had could be the key to the right diagnosis, because sometimes adamantinoma of long bones is associated with a fibrous dysplasia-like pattern. The reason for misinterpretation of the histological features is seen in the typical variable histological pattern of this tumor (basaloids, squamoid, spindled and tubular pattern). In this connection is refered to the necessity to correlate the histological feature with the X-ray in making diagnosis in bone pathology."}
{"id": "PMID:475566", "title": "[Dedifferentiation of chrondrosarcomas (author's transl)].", "content": "The clinical course and the results of radiographic and histological investigations in three patients with dedifferentiated chondrosarcoma are presented. The tumors were characterized by chondrosarcomatous tumor sections of low grade malignancy adjacent to fibrosarcomatous tissue sections. One to two and one-half year after the onset of the first clinical symptoms pulmonary metastases appeared and in one case a subcutaneous mass presented, which had the histological appearance of a fibrosarcoma. Differentiation of a chondrosarcoma customarily has an adverse effect on the prognosis with both the early appearance of metastases and a rapidly fatal clinical course. Cartilagenous tumors of the axial skeleton and the long bones must be extirpated surgically in an early and radical fashion, particularly if there are radiographic signs of proliferation.", "contents": "[Dedifferentiation of chrondrosarcomas (author's transl)]. The clinical course and the results of radiographic and histological investigations in three patients with dedifferentiated chondrosarcoma are presented. The tumors were characterized by chondrosarcomatous tumor sections of low grade malignancy adjacent to fibrosarcomatous tissue sections. One to two and one-half year after the onset of the first clinical symptoms pulmonary metastases appeared and in one case a subcutaneous mass presented, which had the histological appearance of a fibrosarcoma. Differentiation of a chondrosarcoma customarily has an adverse effect on the prognosis with both the early appearance of metastases and a rapidly fatal clinical course. Cartilagenous tumors of the axial skeleton and the long bones must be extirpated surgically in an early and radical fashion, particularly if there are radiographic signs of proliferation."}
{"id": "PMID:475567", "title": "Intramuscular neurolysis for spasticity in children.", "content": "Intramuscular neutrolysis with phenol has been used for 10 years in the management of spasticity in children. Best results depend on fastidious technique and realistic use of the procedure. Sedation or anesthesia was used in all cases -- 5% phenol in water was used for all procedures. The main indications were spasticity which interfered with function, either actual or potential, or with care. Where uninhibited vestibular or tonic neck reflexes affect muscle tone, or there is dystonia or athetosis, the procedure is less effective than where spasticity alone is present. Duration of relief of spasticity ranged from 1 month to more than 2 years. About one half of the lower extremity muscle treated required tenotomy later. Generally training was required after the procedure to obtain improved function. A representative sample of muscles treated, repeat procedures, and later surgery is discussed. The procedure is recommended for use in the management of spasticity in children as a way of improving function and/or care.", "contents": "Intramuscular neurolysis for spasticity in children. Intramuscular neutrolysis with phenol has been used for 10 years in the management of spasticity in children. Best results depend on fastidious technique and realistic use of the procedure. Sedation or anesthesia was used in all cases -- 5% phenol in water was used for all procedures. The main indications were spasticity which interfered with function, either actual or potential, or with care. Where uninhibited vestibular or tonic neck reflexes affect muscle tone, or there is dystonia or athetosis, the procedure is less effective than where spasticity alone is present. Duration of relief of spasticity ranged from 1 month to more than 2 years. About one half of the lower extremity muscle treated required tenotomy later. Generally training was required after the procedure to obtain improved function. A representative sample of muscles treated, repeat procedures, and later surgery is discussed. The procedure is recommended for use in the management of spasticity in children as a way of improving function and/or care."}
{"id": "PMID:475568", "title": "Autonomic hyperreflexia and its differentiation from pheochromocytoma.", "content": "Conceivably pheochromocytoma may have to be differentiated from autonomic hyperreflexia as the cause of paroxysmal hypertension. Elevated urinary catecholamine metabolites may occur in both conditions. However, marked elevations of serum dopamine-beta-hydroxylase (DBH) accompanied by relatively slight elevations of plasma catecholamines are observed during hypertensive paroxysms in patients with autonomic hyperreflexia, while marked elevations of plasma catecholamines with little if any change in serum DBH concentration are characteristic of hypertensive paroxysms caused by pheochromocytoma.", "contents": "Autonomic hyperreflexia and its differentiation from pheochromocytoma. Conceivably pheochromocytoma may have to be differentiated from autonomic hyperreflexia as the cause of paroxysmal hypertension. Elevated urinary catecholamine metabolites may occur in both conditions. However, marked elevations of serum dopamine-beta-hydroxylase (DBH) accompanied by relatively slight elevations of plasma catecholamines are observed during hypertensive paroxysms in patients with autonomic hyperreflexia, while marked elevations of plasma catecholamines with little if any change in serum DBH concentration are characteristic of hypertensive paroxysms caused by pheochromocytoma."}
{"id": "PMID:475569", "title": "Electrode implantation and electrostimulation in rabbits.", "content": "The investigators studied functional electrostimulation by means of an implanted electrode placed around the peroneal nerve in rabbits. Current pulses were administered from a stimulating unit outside the body, via a connecting skin plug and subcutaneous leads to the electrode. It is concluded that if this form of stimulation is to be praticable without undesirable results, certain mechanical problems require further study.", "contents": "Electrode implantation and electrostimulation in rabbits. The investigators studied functional electrostimulation by means of an implanted electrode placed around the peroneal nerve in rabbits. Current pulses were administered from a stimulating unit outside the body, via a connecting skin plug and subcutaneous leads to the electrode. It is concluded that if this form of stimulation is to be praticable without undesirable results, certain mechanical problems require further study."}
{"id": "PMID:475570", "title": "Funicular localization in partial median nerve injury at the wrists.", "content": "The funiculi of the median nerve at the wrist carrying the motor and sensory fibers innervating the hand were described in 1945 by Sunderland after an extensive anatomic study. In an attempt to confirm clinically the findings of that study, 7 patients with partial median nerve laceration at the wrist are presented. The findings in these patients support the schema developed from Sunderland's study except for the funiculi subserving the 1st and 2nd lumbricals which appear to be more dorsally placed. They also tend to confirm Sunderland's finding that the mixing of nerve fibers from different funiculi and the formation of intraneural plexuses occur more proximal than at the levels of injuries in the patients studied.", "contents": "Funicular localization in partial median nerve injury at the wrists. The funiculi of the median nerve at the wrist carrying the motor and sensory fibers innervating the hand were described in 1945 by Sunderland after an extensive anatomic study. In an attempt to confirm clinically the findings of that study, 7 patients with partial median nerve laceration at the wrist are presented. The findings in these patients support the schema developed from Sunderland's study except for the funiculi subserving the 1st and 2nd lumbricals which appear to be more dorsally placed. They also tend to confirm Sunderland's finding that the mixing of nerve fibers from different funiculi and the formation of intraneural plexuses occur more proximal than at the levels of injuries in the patients studied."}
{"id": "PMID:475571", "title": "Fractures in patients with myopathies.", "content": "During the past 4 years, 15 fractures in 10 patients were encountered among 48 hospitalized patients with myopathies. The dominant sites were the femur (supracondylar) and humerus (subcapital). By assessing the total body potassium volume, bone mineral content, serum calcium, inorganic phosphorus, 25-OH vitamin D3, parathyroid hormone, and calcitonin, it is concluded that the proclivity to fracture in myopathic patients is caused by bone atrophy due to a lack of muscle tension related to decrease in muscle volume.", "contents": "Fractures in patients with myopathies. During the past 4 years, 15 fractures in 10 patients were encountered among 48 hospitalized patients with myopathies. The dominant sites were the femur (supracondylar) and humerus (subcapital). By assessing the total body potassium volume, bone mineral content, serum calcium, inorganic phosphorus, 25-OH vitamin D3, parathyroid hormone, and calcitonin, it is concluded that the proclivity to fracture in myopathic patients is caused by bone atrophy due to a lack of muscle tension related to decrease in muscle volume."}
{"id": "PMID:475572", "title": "Lumbar fracture-dislocation related to range-of-motion exercises.", "content": "Range-of-motion exercises are an established and widely accepted therapeutic modality in the treatment of the person with spinal cord injury and are usually uneventful. Occasionally, however, accidents do occur; among them are fractures and dislocations of limbs. An unusual \"slice\" fracture of L4, related to range of motion exercises, occurred in a young man who was a quadriplegic and converted him from upper to lower motor neuron bowel, bladder and sexual function. This iatrogenic fracture, although extremely rare, can be prevented if symptoms are properly interpreted.", "contents": "Lumbar fracture-dislocation related to range-of-motion exercises. Range-of-motion exercises are an established and widely accepted therapeutic modality in the treatment of the person with spinal cord injury and are usually uneventful. Occasionally, however, accidents do occur; among them are fractures and dislocations of limbs. An unusual \"slice\" fracture of L4, related to range of motion exercises, occurred in a young man who was a quadriplegic and converted him from upper to lower motor neuron bowel, bladder and sexual function. This iatrogenic fracture, although extremely rare, can be prevented if symptoms are properly interpreted."}
{"id": "PMID:475574", "title": "Adjustment of homosexual and heterosexual women: a cross-national replication.", "content": "British homosexual and heterosexual women were evaluated on the same adjustment inventories used with two similar U.S. groups. The results of both studies were quite parallel, and the findings again supported similarities rather than differences in adjustment between homosexuals and heterosexuals.", "contents": "Adjustment of homosexual and heterosexual women: a cross-national replication. British homosexual and heterosexual women were evaluated on the same adjustment inventories used with two similar U.S. groups. The results of both studies were quite parallel, and the findings again supported similarities rather than differences in adjustment between homosexuals and heterosexuals."}
{"id": "PMID:475573", "title": "Sex hormones and female homosexuality: a critical examination.", "content": "To ascertain the validity of hormonal theories of human homosexuality, which are based on animal research, this article reviews psychoendocrine data on lesbian and transsexual women. Sex hormone levels were found to be normal in the majority of homosexual women, but about a third of the subjects studied had elevated androgen levels. In women with prenatal androgen excess, heterosexuality appears to be more frequent than bisexuality, and exclusive homosexuality is rare. Two recent reports suggest abnormalities of the neuroendocrine regulation of LH secretion in female transsexuals. Clearly, prenatal or postpubertal hormone levels do not determine the development of sexual orientation, but a facilitating neuroendocrine predisposition cannot be ruled out at present.", "contents": "Sex hormones and female homosexuality: a critical examination. To ascertain the validity of hormonal theories of human homosexuality, which are based on animal research, this article reviews psychoendocrine data on lesbian and transsexual women. Sex hormone levels were found to be normal in the majority of homosexual women, but about a third of the subjects studied had elevated androgen levels. In women with prenatal androgen excess, heterosexuality appears to be more frequent than bisexuality, and exclusive homosexuality is rare. Two recent reports suggest abnormalities of the neuroendocrine regulation of LH secretion in female transsexuals. Clearly, prenatal or postpubertal hormone levels do not determine the development of sexual orientation, but a facilitating neuroendocrine predisposition cannot be ruled out at present."}
{"id": "PMID:475575", "title": "Group treatment of single males with erectile dysfunction.", "content": "Nine men with chronic erectile dysfunction (three primary, six secondary) who had no regular sexual partner were treated in two 12-session all-male psychoeducational therapy groups. Treatment intervention addressed specific factors which inhibited adequate sexual function with a focus on coping skills to overcome those factors. Pre, post, and follow-up behavioral self-report data and responses on a goal attainment scale questionnaire indicated that the treatment groups were successful for five men with secondary and one man with primary erectile dysfunction. Subjective report and pre- and posttreatment fantasy productions to TAT cards for the first group indicated that all men significantly improved their attitudes about sexuality and their sexual self-concept. The results suggest that this is a viable, cost-effective treatment for secondary erectile dysfunction, but not for primary erectile dysfunction unless supplementary individual therapy is provided.", "contents": "Group treatment of single males with erectile dysfunction. Nine men with chronic erectile dysfunction (three primary, six secondary) who had no regular sexual partner were treated in two 12-session all-male psychoeducational therapy groups. Treatment intervention addressed specific factors which inhibited adequate sexual function with a focus on coping skills to overcome those factors. Pre, post, and follow-up behavioral self-report data and responses on a goal attainment scale questionnaire indicated that the treatment groups were successful for five men with secondary and one man with primary erectile dysfunction. Subjective report and pre- and posttreatment fantasy productions to TAT cards for the first group indicated that all men significantly improved their attitudes about sexuality and their sexual self-concept. The results suggest that this is a viable, cost-effective treatment for secondary erectile dysfunction, but not for primary erectile dysfunction unless supplementary individual therapy is provided."}
{"id": "PMID:475577", "title": "Coital and contraceptive behavior of female adolescents.", "content": "Structural interviews conducted with 532 female clients of a teen contraceptive clinic revealed a moderate level of coital activity i(in terms of both frequency of intercourse and number of partners) which typically began at age 15. Prior use of contraception was sporadic and almost exclusively confined to nonprescription methods. Primary reasons for nonuse of birth control included fear of lessening the pleasure of intercourse and lack of access to contraception. The data indicate that a combination of comprehensive sex education and ready access to birth control is necessary to prevent unintended adolescent pregnancy.", "contents": "Coital and contraceptive behavior of female adolescents. Structural interviews conducted with 532 female clients of a teen contraceptive clinic revealed a moderate level of coital activity i(in terms of both frequency of intercourse and number of partners) which typically began at age 15. Prior use of contraception was sporadic and almost exclusively confined to nonprescription methods. Primary reasons for nonuse of birth control included fear of lessening the pleasure of intercourse and lack of access to contraception. The data indicate that a combination of comprehensive sex education and ready access to birth control is necessary to prevent unintended adolescent pregnancy."}
{"id": "PMID:475578", "title": "Sexual response in spinal cord injured adults: a review of the literature.", "content": "The literature on sexual response in spinal cord injured (SCI) adults is reviewed and criticized. Principal characteristics of spinal cord injury are reviewed, and variables usually taken into account in studying sexual behavior in the SCI population are specified. Target behaviors of erection, ejaculation, attempts at intercourse, and male and female orgasm are isolated, and relevant data are reported. Attention is given to conceptual and methodological shortcomings of past research. Literature relevant to remediation of sexual problems in the SCI population is reported, and outcomes are noted. Future directions for research are indicated with particular emphasis placed on the appropriate utilization of psychophysiological techniques.", "contents": "Sexual response in spinal cord injured adults: a review of the literature. The literature on sexual response in spinal cord injured (SCI) adults is reviewed and criticized. Principal characteristics of spinal cord injury are reviewed, and variables usually taken into account in studying sexual behavior in the SCI population are specified. Target behaviors of erection, ejaculation, attempts at intercourse, and male and female orgasm are isolated, and relevant data are reported. Attention is given to conceptual and methodological shortcomings of past research. Literature relevant to remediation of sexual problems in the SCI population is reported, and outcomes are noted. Future directions for research are indicated with particular emphasis placed on the appropriate utilization of psychophysiological techniques."}
{"id": "PMID:475579", "title": "Experimental studies of the etiology of genital exhibitionism.", "content": "Several studies are reported which examine the critical stimuli and responses as well as personality factors important in genital exhibitionism. Using penile volume measurement, sex history questionnaires, personality tests, and behavioral observations, it was found that (1) exhibitionists responded most to mature females and were comparable to normals in reactions to children and men; (2) while exhibitionists indicated narcissistic desires in exposing, in a laboratory study they did not differ from controls in reactions to exposing with various reactions of the female, from sexual arousal and admiration to fear and anger; (3) the only strong features distinguishing exhibitionists from controls and other sexually anomalous subjects were peeping associated with orgasm and outdoor solitary masturbation; (4) exhibitionists seemed to be less assertive and less feminine than controls, but results were weak and inconsistent; (5) exhibitionists and controls did not differ in separation and divorce or marital satisfaction; (6) blood testosterone and penile reactivity of exhibitionists were within normal limits. Overall, the results suggest that most major theories of genital exhibitionism are wanting. It is suggested that narcissism and pedophilia be examined further in connection with exhibitionism.", "contents": "Experimental studies of the etiology of genital exhibitionism. Several studies are reported which examine the critical stimuli and responses as well as personality factors important in genital exhibitionism. Using penile volume measurement, sex history questionnaires, personality tests, and behavioral observations, it was found that (1) exhibitionists responded most to mature females and were comparable to normals in reactions to children and men; (2) while exhibitionists indicated narcissistic desires in exposing, in a laboratory study they did not differ from controls in reactions to exposing with various reactions of the female, from sexual arousal and admiration to fear and anger; (3) the only strong features distinguishing exhibitionists from controls and other sexually anomalous subjects were peeping associated with orgasm and outdoor solitary masturbation; (4) exhibitionists seemed to be less assertive and less feminine than controls, but results were weak and inconsistent; (5) exhibitionists and controls did not differ in separation and divorce or marital satisfaction; (6) blood testosterone and penile reactivity of exhibitionists were within normal limits. Overall, the results suggest that most major theories of genital exhibitionism are wanting. It is suggested that narcissism and pedophilia be examined further in connection with exhibitionism."}
{"id": "PMID:475580", "title": "Klinefelter's syndrome: sexual development and activity.", "content": "Determination of plasma testosterone in 105 patients with Klinefelter's syndrome 16--45 years of age revealed that at each 5-year age interval values of male hormone were lower than in a control group of 25 normal adolescents and 85 fertile and potent men. Analysis of heterosexual development by means of the HTDM Questionnaire in 110 patients with Klinefelter's syndrome age 21--40 years, who were examined mainly for sterility and signs of imperfect somatosexual development, revealed a distinct retardation of sociosexual development compared to that of 325 normozoospermic men from sterile marriages. For eight out of 12 items on the HTDM Questionnaire the differences were statistically significant. Examination by means of the SAM Questionnaire in these two groups revealed that the sexual activity of examinees with Klinefelter's syndrome was significantly weaker than that of fertile and potent men. For 15 out of 18 items on the SAM Questionnaire the differences were statistically significant.", "contents": "Klinefelter's syndrome: sexual development and activity. Determination of plasma testosterone in 105 patients with Klinefelter's syndrome 16--45 years of age revealed that at each 5-year age interval values of male hormone were lower than in a control group of 25 normal adolescents and 85 fertile and potent men. Analysis of heterosexual development by means of the HTDM Questionnaire in 110 patients with Klinefelter's syndrome age 21--40 years, who were examined mainly for sterility and signs of imperfect somatosexual development, revealed a distinct retardation of sociosexual development compared to that of 325 normozoospermic men from sterile marriages. For eight out of 12 items on the HTDM Questionnaire the differences were statistically significant. Examination by means of the SAM Questionnaire in these two groups revealed that the sexual activity of examinees with Klinefelter's syndrome was significantly weaker than that of fertile and potent men. For 15 out of 18 items on the SAM Questionnaire the differences were statistically significant."}
{"id": "PMID:475581", "title": "Ego development and sex attitudes in heterosexual and homosexual men and women.", "content": "A comparison was made of heterosexual and homosexual men and women utilizing Loevinger's concept of ego development and focusing on the relationships among ego levels and attitudes toward homosexuality and on personal sex guilt and other sociosexual variables. Previous comparative studies were critically analyzed with respect to the adequacy of definition and description of sampling procedures, and the appropriateness of research question. A pilot study reported here concerns the development of a measure of \"attitudes toward homosexuality.\" The major study utilized 200 subjects who completed an anonymous and lengthy questionnaire. This sample was relatively young, well-educated, white, and Protestant. Self-ratings on the two Kinsey-type scales clearly differentiated the self-identified primarily heterosexual and homosexual groups. In this study there were no significant differences in ego levels between the heterosexual and the homosexual groups, suggesting that ego development and sexual orientation development are independent phenomena. Among both homosexual and heterosexual subjects there were low but significant correlations between higher levels of ego development and more positive attitudes toward homosexuality. More negative attitudes toward homosexuality were correlated with higher levels of personal sex guilt for heterosexual and homosexual men and for heterosexual women. Our measure of sex guilt proved to be related to ego level only for heterosexual males, although the measure of this construct requires additional research and refinement. Other correlates of these variables are also reported.", "contents": "Ego development and sex attitudes in heterosexual and homosexual men and women. A comparison was made of heterosexual and homosexual men and women utilizing Loevinger's concept of ego development and focusing on the relationships among ego levels and attitudes toward homosexuality and on personal sex guilt and other sociosexual variables. Previous comparative studies were critically analyzed with respect to the adequacy of definition and description of sampling procedures, and the appropriateness of research question. A pilot study reported here concerns the development of a measure of \"attitudes toward homosexuality.\" The major study utilized 200 subjects who completed an anonymous and lengthy questionnaire. This sample was relatively young, well-educated, white, and Protestant. Self-ratings on the two Kinsey-type scales clearly differentiated the self-identified primarily heterosexual and homosexual groups. In this study there were no significant differences in ego levels between the heterosexual and the homosexual groups, suggesting that ego development and sexual orientation development are independent phenomena. Among both homosexual and heterosexual subjects there were low but significant correlations between higher levels of ego development and more positive attitudes toward homosexuality. More negative attitudes toward homosexuality were correlated with higher levels of personal sex guilt for heterosexual and homosexual men and for heterosexual women. Our measure of sex guilt proved to be related to ego level only for heterosexual males, although the measure of this construct requires additional research and refinement. Other correlates of these variables are also reported."}
{"id": "PMID:475582", "title": "Vaginal photoplethysmography: methodological considerations.", "content": "Methodological considerations related to the use of vaginal photoplethysmography in the experimental investigation of physiological sexual arousal in women are discussed. Specific topics covered include the operation and use of the device, methods of data reduction and analysis, and the role of vaginal photoplethysmography in the study of female sexual arousal. A review of the literature focusing on methodological issues is also included.", "contents": "Vaginal photoplethysmography: methodological considerations. Methodological considerations related to the use of vaginal photoplethysmography in the experimental investigation of physiological sexual arousal in women are discussed. Specific topics covered include the operation and use of the device, methods of data reduction and analysis, and the role of vaginal photoplethysmography in the study of female sexual arousal. A review of the literature focusing on methodological issues is also included."}
{"id": "PMID:475583", "title": "The intelligence of rapists: new data.", "content": "IQs of violent sexual offenders (rapists) were compared to those of alleged nonviolent sexual offenders, nonsexual violent offenders, and nonsexual nonviolent offenders. Data were gathered from defendants referred to the medical coauthor for pretrial psychiatric evaluation. Rapists scored lower in the IQ test, but this difference did not appear to be significant in the pairwise X2 comparisons. This held true after controlling for the race of the defendant.", "contents": "The intelligence of rapists: new data. IQs of violent sexual offenders (rapists) were compared to those of alleged nonviolent sexual offenders, nonsexual violent offenders, and nonsexual nonviolent offenders. Data were gathered from defendants referred to the medical coauthor for pretrial psychiatric evaluation. Rapists scored lower in the IQ test, but this difference did not appear to be significant in the pairwise X2 comparisons. This held true after controlling for the race of the defendant."}
{"id": "PMID:475584", "title": "Exorcism as psychiatric treatment: a homosexual case study.", "content": "A case of exorcism as apparently successful treatment of transsexualism has previously been reported. The actual procedure in a similar case of exorcism by a psychiatrist is reported, together with details of the outcome and a discussion of theoretical, applied, and ethical aspects of the case.", "contents": "Exorcism as psychiatric treatment: a homosexual case study. A case of exorcism as apparently successful treatment of transsexualism has previously been reported. The actual procedure in a similar case of exorcism by a psychiatrist is reported, together with details of the outcome and a discussion of theoretical, applied, and ethical aspects of the case."}
{"id": "PMID:475585", "title": "[Influence of native crude fibers on the true digestibility of nitrogen and the amino acids].", "content": "In several test series with rats, the influence of native crude fibres (straw meal and pea hulls) on the amount of metabolic fecal nitrogen (MFN) was tested. It could be ascertained that native crude fibres increase MFN. The influence of the increased MFN on the true digestibility of nitrogen and the amino acids was investigated in test series with casein diets. The casein diets were supplemented with native crude fibres. Generalising, we can state that native crude fibres--due to their structure (lignin)--fix amino acids and thus withhold them from absorption. They do not, however, increase the MFN by mechanical action (increase of the scraping off of intestinal epithelium).", "contents": "[Influence of native crude fibers on the true digestibility of nitrogen and the amino acids]. In several test series with rats, the influence of native crude fibres (straw meal and pea hulls) on the amount of metabolic fecal nitrogen (MFN) was tested. It could be ascertained that native crude fibres increase MFN. The influence of the increased MFN on the true digestibility of nitrogen and the amino acids was investigated in test series with casein diets. The casein diets were supplemented with native crude fibres. Generalising, we can state that native crude fibres--due to their structure (lignin)--fix amino acids and thus withhold them from absorption. They do not, however, increase the MFN by mechanical action (increase of the scraping off of intestinal epithelium)."}
{"id": "PMID:475586", "title": "[Food value of straw meal in the nutrition of pigs. 1. Digestibility of the nutrients and N-balance after feeding with partially hydrolized straw meal].", "content": "Straw Meal was mixed with concentrated HCl at a mass proportion of approximately 10 : 1. After a one-hour duration of action the mixture was diluted with water (in proporation to the added HCl volume the double amount of water) and steamed in an electric steaming caldron for one hour. The partially hydrolised final product was neutralised with calcium hydroxide (3,5 kg per 10 kg HCl). In a digestibility experiment 4 pigs were fed according to the differential method with barley (supplemented with lysine and methionine) without straw meal resp. with straw meal (717 g barley dry matter+386 g straw meal dry matter per animal and day for pigs with an average live weight of 64.3 kg). In this test 21.0% of the organic matter of partially hydrolised straw meal, 0.5% of the crude fibre and 42.3% of the N-free extractives were digestible. When the animals were fed with barley exclusively (1045 g dry matter per day and animal of an average body weight of 61,4 kg), 17,1% of the 26,0 t N per animal and day were excreted in feces. After the feeding with straw meal admixtures, 34,5% of the 20,9 g N per animal and day were excreted in feces. When the feed consisted of 1,99 kg barley, 220 g fish meal and 565 g straw meal (dry matter in each case) per animal and day for the same pigs with an average body weight of 97,1 kg a digestibility of the organic matter of the partially hydrolised straw meal of 24,6% was achieved. 24.3% of the nitrogen consumed (72.9 g) by each animal per day were excreted in feces. After slaughtering, the pH-value in the caecum sould be ascertained as 6.0 to 6.5. The conclusion of this is that partially hydrolised straw meal contains approximately 20% utilisable carbohydrates and has a positive dietic effect in the digestive tract.", "contents": "[Food value of straw meal in the nutrition of pigs. 1. Digestibility of the nutrients and N-balance after feeding with partially hydrolized straw meal]. Straw Meal was mixed with concentrated HCl at a mass proportion of approximately 10 : 1. After a one-hour duration of action the mixture was diluted with water (in proporation to the added HCl volume the double amount of water) and steamed in an electric steaming caldron for one hour. The partially hydrolised final product was neutralised with calcium hydroxide (3,5 kg per 10 kg HCl). In a digestibility experiment 4 pigs were fed according to the differential method with barley (supplemented with lysine and methionine) without straw meal resp. with straw meal (717 g barley dry matter+386 g straw meal dry matter per animal and day for pigs with an average live weight of 64.3 kg). In this test 21.0% of the organic matter of partially hydrolised straw meal, 0.5% of the crude fibre and 42.3% of the N-free extractives were digestible. When the animals were fed with barley exclusively (1045 g dry matter per day and animal of an average body weight of 61,4 kg), 17,1% of the 26,0 t N per animal and day were excreted in feces. After the feeding with straw meal admixtures, 34,5% of the 20,9 g N per animal and day were excreted in feces. When the feed consisted of 1,99 kg barley, 220 g fish meal and 565 g straw meal (dry matter in each case) per animal and day for the same pigs with an average body weight of 97,1 kg a digestibility of the organic matter of the partially hydrolised straw meal of 24,6% was achieved. 24.3% of the nitrogen consumed (72.9 g) by each animal per day were excreted in feces. After slaughtering, the pH-value in the caecum sould be ascertained as 6.0 to 6.5. The conclusion of this is that partially hydrolised straw meal contains approximately 20% utilisable carbohydrates and has a positive dietic effect in the digestive tract."}
{"id": "PMID:475587", "title": "[Metabolism of colostomized laying hens with 15N-labeled wheat. 2. Incorporation of the labeled nitrogen into fractions of the egg].", "content": "The colostomised hens received 15N-labelled wheat. For a period of 8 days the incorporation of the 15N excess (15N') into the albumen, the yolk and the egg-shell was measured. In addition to that, the values for atom-percent of 15N' in the amino acids lysine, histidien and arginine of the albumen and the yolk were ascertained. The egg-shell and the albumen were labelled shortly after the beginning of the experiment. In both fractions the atom-per cent of 15N' rises more quickly than in the yolk. After the withdrawal of the labelled feed the decrease in the yolk was slower than in the egg-shell and the albumen. Concerning the atom-per cent of 15N' there is hardly a difference between the total N and the amino acids. Despite the different supply with amino acids, the relation of histidine 15N' : lysine 15N' : arginine-15N' in the yolk and the albumen remains largely constant. It is 1 : 2 : 3 and corresponds to the quantitative relation of the N content of the three amino acids in the egg protein. The utilisation of 14N and 15N' of the wheat lysin for the egg synthesis amounts to approximately 50%. There are considerable differences between the utilisation of 15N' of the histidine and 15N' of the arginine and the 14N of the two dietary amino acids.", "contents": "[Metabolism of colostomized laying hens with 15N-labeled wheat. 2. Incorporation of the labeled nitrogen into fractions of the egg]. The colostomised hens received 15N-labelled wheat. For a period of 8 days the incorporation of the 15N excess (15N') into the albumen, the yolk and the egg-shell was measured. In addition to that, the values for atom-percent of 15N' in the amino acids lysine, histidien and arginine of the albumen and the yolk were ascertained. The egg-shell and the albumen were labelled shortly after the beginning of the experiment. In both fractions the atom-per cent of 15N' rises more quickly than in the yolk. After the withdrawal of the labelled feed the decrease in the yolk was slower than in the egg-shell and the albumen. Concerning the atom-per cent of 15N' there is hardly a difference between the total N and the amino acids. Despite the different supply with amino acids, the relation of histidine 15N' : lysine 15N' : arginine-15N' in the yolk and the albumen remains largely constant. It is 1 : 2 : 3 and corresponds to the quantitative relation of the N content of the three amino acids in the egg protein. The utilisation of 14N and 15N' of the wheat lysin for the egg synthesis amounts to approximately 50%. There are considerable differences between the utilisation of 15N' of the histidine and 15N' of the arginine and the 14N of the two dietary amino acids."}
{"id": "PMID:475589", "title": "[Utilization of feed energy by growing pigs. 3. Energy requirement for the growth and fattening of pigs].", "content": "The test series for the investigation of the energy consumption of growing pigs of the breeds large white and improved land race pig as well as cross breeds of the two breeds in a total of 369 metabolism periods (as described in the first two pieces of information of this publication series -- Hoffmann and others, 1977 and Jentsch and Hoffmann, 1977) were statistically analysed for the purpose of the derivation of the energy requirement for maintenance and the partial energy requirement for growth in order to test the possibilities of the factorial analysis for the derivation of energy requirement values of growing pigs. The dependence of the maintenance requirement of growing pigs (investigations in the live weight range of 10 to 40 kg -- see 1st information--were made with boars those in the live weight range of 30 to 120 kg were made with gelded boars, 2nd information) on the live weight can best be characterised by applying a power exponent of 0,61 or 0,62 for the live weight. A definition is offered to be discussed for the energetic maintenance requirement of productive live stock and laboratory animals as a conventional value. The energy requirement values derived from the doubly-factorial statistical analysis show a satisfactory adaptation to the measured values as such concerning energy intake and observed growth performance of the test animals. The conclusion is drawn that the factorial analysis of the energy requirement (maintenance plus partial performances) results in a better estimate of the requirement of growing animals than the assessment according only to live weight and live weight increase without characterising the energy requirement for partial performances. This is important for the further working on and more exact definition of requirement norms.", "contents": "[Utilization of feed energy by growing pigs. 3. Energy requirement for the growth and fattening of pigs]. The test series for the investigation of the energy consumption of growing pigs of the breeds large white and improved land race pig as well as cross breeds of the two breeds in a total of 369 metabolism periods (as described in the first two pieces of information of this publication series -- Hoffmann and others, 1977 and Jentsch and Hoffmann, 1977) were statistically analysed for the purpose of the derivation of the energy requirement for maintenance and the partial energy requirement for growth in order to test the possibilities of the factorial analysis for the derivation of energy requirement values of growing pigs. The dependence of the maintenance requirement of growing pigs (investigations in the live weight range of 10 to 40 kg -- see 1st information--were made with boars those in the live weight range of 30 to 120 kg were made with gelded boars, 2nd information) on the live weight can best be characterised by applying a power exponent of 0,61 or 0,62 for the live weight. A definition is offered to be discussed for the energetic maintenance requirement of productive live stock and laboratory animals as a conventional value. The energy requirement values derived from the doubly-factorial statistical analysis show a satisfactory adaptation to the measured values as such concerning energy intake and observed growth performance of the test animals. The conclusion is drawn that the factorial analysis of the energy requirement (maintenance plus partial performances) results in a better estimate of the requirement of growing animals than the assessment according only to live weight and live weight increase without characterising the energy requirement for partial performances. This is important for the further working on and more exact definition of requirement norms."}
{"id": "PMID:475590", "title": "Molecular mechanism of 1,1-dichloroethylene toxicity: excreted metabolites reveal different pathways of reactive intermediates.", "content": "The excretion and biotransformation of [14C] 1,1-dichloroethylene (vinylidene chloride, VDC) after administration of a single oral dose has been investigated in female rats. Seventy-two hours after a dose of 0.5, 5.0, and 50.0 mg/kg, 1.26, 9.70, 16.47%, respectively, are exhaled as unchanged VDC, and 13.64, 11.35, 6.13% as 14CO2. The main pathway of elimination is through renal excretion with 43.55, 53.88, 42.11% of the administered radioactivity. Through the biliary system, 15.74, 14.54, 7.65% of the activity are eliminated. The isolation of the main metabolites of VDC from 24 h urine is accomplished through the combined application of solvent extraction, ion exchange chromatography and thin layer chromatography. Then gas chromatography and mass spectrometry are used for their identification. Three metabolites have been identified: thiodiglycolic acid, N-acetyl-S-(2-carboxymethyl)cysteine and methyl-thio-acetylaminoethanol. In addition, three smaller unidentified radioactive peaks have been found. Thiodiglycolic acid is the main metabolite in VDC metabolism. The simultaneous formation of an ethanolamine- and a cysteine-conjugation product points to different reaction pathways of the postulated intermediate reactive epoxide; ethanolamine probably originates from membrane lipids, which react with VDC-epoxide and/or its derivatives. This pathway could explain, in part, the parenchyma damaging effect of VDC.", "contents": "Molecular mechanism of 1,1-dichloroethylene toxicity: excreted metabolites reveal different pathways of reactive intermediates. The excretion and biotransformation of [14C] 1,1-dichloroethylene (vinylidene chloride, VDC) after administration of a single oral dose has been investigated in female rats. Seventy-two hours after a dose of 0.5, 5.0, and 50.0 mg/kg, 1.26, 9.70, 16.47%, respectively, are exhaled as unchanged VDC, and 13.64, 11.35, 6.13% as 14CO2. The main pathway of elimination is through renal excretion with 43.55, 53.88, 42.11% of the administered radioactivity. Through the biliary system, 15.74, 14.54, 7.65% of the activity are eliminated. The isolation of the main metabolites of VDC from 24 h urine is accomplished through the combined application of solvent extraction, ion exchange chromatography and thin layer chromatography. Then gas chromatography and mass spectrometry are used for their identification. Three metabolites have been identified: thiodiglycolic acid, N-acetyl-S-(2-carboxymethyl)cysteine and methyl-thio-acetylaminoethanol. In addition, three smaller unidentified radioactive peaks have been found. Thiodiglycolic acid is the main metabolite in VDC metabolism. The simultaneous formation of an ethanolamine- and a cysteine-conjugation product points to different reaction pathways of the postulated intermediate reactive epoxide; ethanolamine probably originates from membrane lipids, which react with VDC-epoxide and/or its derivatives. This pathway could explain, in part, the parenchyma damaging effect of VDC."}
{"id": "PMID:475591", "title": "Induction of dominant lethal mutations in male mice by fosfestrol.", "content": "Using the dominant lethal assay, the ability of fosfestrol, a diethylstilboestrol derivate, to induce mutations in male mice was tested and confirmed. Up to 300 mg/kg of fosfestrol the induction of mutations occurs exclusively in spermatozoa. A dose of 600 mg/kg of fosfestrol induces dominant lethal mutations up to 10 days posttreatment. The majority of induced dominant lethal mutations in all dose groups are expressed as loss after implantation.", "contents": "Induction of dominant lethal mutations in male mice by fosfestrol. Using the dominant lethal assay, the ability of fosfestrol, a diethylstilboestrol derivate, to induce mutations in male mice was tested and confirmed. Up to 300 mg/kg of fosfestrol the induction of mutations occurs exclusively in spermatozoa. A dose of 600 mg/kg of fosfestrol induces dominant lethal mutations up to 10 days posttreatment. The majority of induced dominant lethal mutations in all dose groups are expressed as loss after implantation."}
{"id": "PMID:475592", "title": "The effects of aspirin on the development of the mouse third molar. A potential screening system for weak teratogens.", "content": "Aspirin is teratogenic at high doses in rodents. A dose related effect is shown at much lower levels in an animal testing system which permits subthreshold effects on development to be measured. This system, which depends on changes in tooth size in the mouse, has advantages in assessing weak or cumulative teratogens.", "contents": "The effects of aspirin on the development of the mouse third molar. A potential screening system for weak teratogens. Aspirin is teratogenic at high doses in rodents. A dose related effect is shown at much lower levels in an animal testing system which permits subthreshold effects on development to be measured. This system, which depends on changes in tooth size in the mouse, has advantages in assessing weak or cumulative teratogens."}
{"id": "PMID:475593", "title": "Effects of dichlorvos (DDVP) inhalation on the activity of acetylcholinesterase in the bronchial tissue of rats.", "content": "The experiments presented here deal with the effects of the inhalation of dichlorvos [dimethyl-(2,2 dichlorvinyl)-phosphate, DDVP] vapor on acetylcholinesterase (ACHE) activity in rat bronchial tissue. Exposure to DDVP concentrations of 0.8 and 1.8 micrograms/l for 3 days reduced ACHE activity in the bronchial tissue (62.8 +/- 0.8 and 51.6 +/- 1.6% of the control), but did not elicit any changes in blood ACHE activity (101 +/- 4.5% of the control each). Higher concentrations (4.3 micrograms/l) induced a decline in ACHE activity also in the blood (38.2 +/- 1.1% of the control). In the histochemical preparations used to demonstrate ACHE activity in bronchial tissue (thiolacetic acid method), a staining of the bronchial glands and smooth muscles characteristic of the enzyme activity was strongly reduced after exposure of the animals to even the lowest dose applied (0.2 microgram/l). The question of whether localized inhibition of ACHE in the bronchial tissue might cause increases in airway resistance due to activation of a broncho-bronchial reflex is discussed. This efferent cholinergic mechanism has been found to be at least partly responsible for maintenance of bronchospasm and hypersecretion in chronic obstructive diseases of the respiratory system.", "contents": "Effects of dichlorvos (DDVP) inhalation on the activity of acetylcholinesterase in the bronchial tissue of rats. The experiments presented here deal with the effects of the inhalation of dichlorvos [dimethyl-(2,2 dichlorvinyl)-phosphate, DDVP] vapor on acetylcholinesterase (ACHE) activity in rat bronchial tissue. Exposure to DDVP concentrations of 0.8 and 1.8 micrograms/l for 3 days reduced ACHE activity in the bronchial tissue (62.8 +/- 0.8 and 51.6 +/- 1.6% of the control), but did not elicit any changes in blood ACHE activity (101 +/- 4.5% of the control each). Higher concentrations (4.3 micrograms/l) induced a decline in ACHE activity also in the blood (38.2 +/- 1.1% of the control). In the histochemical preparations used to demonstrate ACHE activity in bronchial tissue (thiolacetic acid method), a staining of the bronchial glands and smooth muscles characteristic of the enzyme activity was strongly reduced after exposure of the animals to even the lowest dose applied (0.2 microgram/l). The question of whether localized inhibition of ACHE in the bronchial tissue might cause increases in airway resistance due to activation of a broncho-bronchial reflex is discussed. This efferent cholinergic mechanism has been found to be at least partly responsible for maintenance of bronchospasm and hypersecretion in chronic obstructive diseases of the respiratory system."}
{"id": "PMID:475594", "title": "Spontaneous activity of autonomic functions in coma due to drug overdose.", "content": "Spontaneous heart rate, respiration rate and electrodermal activity were studied in 32 patients with different grades of severe poisoning by ingestion of various drugs. The degree of their behavioural impairment was determined with the Munich Coma Scale (MCS) which measures stimulus evoked somatomotor responses. There was a significant reduction of spontaneous variations of heart rate and respiration rate with increasing severity of coma. Spontaneous electrodermal fluctuations were present only at the least severe grades of impairment. Thus, cardiorespiratory and electrodermal activity can provide significant information on the level of behavioural impairment in severe poisoning.", "contents": "Spontaneous activity of autonomic functions in coma due to drug overdose. Spontaneous heart rate, respiration rate and electrodermal activity were studied in 32 patients with different grades of severe poisoning by ingestion of various drugs. The degree of their behavioural impairment was determined with the Munich Coma Scale (MCS) which measures stimulus evoked somatomotor responses. There was a significant reduction of spontaneous variations of heart rate and respiration rate with increasing severity of coma. Spontaneous electrodermal fluctuations were present only at the least severe grades of impairment. Thus, cardiorespiratory and electrodermal activity can provide significant information on the level of behavioural impairment in severe poisoning."}
{"id": "PMID:475595", "title": "The influence of 2-/o-cresyl/-4 H-1 : 3 : 2-benzodioxa-phosphorin-2-oxide (CBDP) on organophosphate poisoning and its therapy.", "content": "The aim of the experiments was to obtain more information on the toxicity of organophosphates and protection against them. Pretreatment of mice with CBDP increased the s.c. toxicity of soman 19.1-fold, and its i.p. toxicity 17.8-fold. The protective effect of atropine and the oximes HS-3, HS-6 and HI-6 in soman poisoning was much greater in CBDP pretreated than in control animals. Atropine + HI-6 raised the s.c. LD50 of soman in the CBDP pretreated animals from 6.8 micrograms/kg to 166 micrograms/kg (PI = 24.3), but in control animals the i.p. LD50 was only raised from 370 micrograms/kg to 608 micrograms/kg (PI = 0.6). CBDP inhibited blood and brain AChE activity, but had no effect on aliesterase (AE) activity in plasma, liver and brain of mice in vivo. CBDP increased s.d. toxicities of sarin 11-fold, of tabun 5-fold and of VX 0.24-fold. The protective index of atropine + HS-3 in sarin poisoning, as in the case of soman poisoning, was much higher in CBDP pretreated than in control animals (20.1 : 13.6), only slightly higher in tabun poisoning (4.3 : 3.4) and in the case of VX poisoning lower in CBDP pretreated than in control animals (32 : 47). The results indicate that CBDP potentiates soman, sarin and tabun toxicities mainly by blocking their binding to non-specific sites in the body.", "contents": "The influence of 2-/o-cresyl/-4 H-1 : 3 : 2-benzodioxa-phosphorin-2-oxide (CBDP) on organophosphate poisoning and its therapy. The aim of the experiments was to obtain more information on the toxicity of organophosphates and protection against them. Pretreatment of mice with CBDP increased the s.c. toxicity of soman 19.1-fold, and its i.p. toxicity 17.8-fold. The protective effect of atropine and the oximes HS-3, HS-6 and HI-6 in soman poisoning was much greater in CBDP pretreated than in control animals. Atropine + HI-6 raised the s.c. LD50 of soman in the CBDP pretreated animals from 6.8 micrograms/kg to 166 micrograms/kg (PI = 24.3), but in control animals the i.p. LD50 was only raised from 370 micrograms/kg to 608 micrograms/kg (PI = 0.6). CBDP inhibited blood and brain AChE activity, but had no effect on aliesterase (AE) activity in plasma, liver and brain of mice in vivo. CBDP increased s.d. toxicities of sarin 11-fold, of tabun 5-fold and of VX 0.24-fold. The protective index of atropine + HS-3 in sarin poisoning, as in the case of soman poisoning, was much higher in CBDP pretreated than in control animals (20.1 : 13.6), only slightly higher in tabun poisoning (4.3 : 3.4) and in the case of VX poisoning lower in CBDP pretreated than in control animals (32 : 47). The results indicate that CBDP potentiates soman, sarin and tabun toxicities mainly by blocking their binding to non-specific sites in the body."}
{"id": "PMID:475596", "title": "[On the effect of cadmium on tissue respiration and gluconeogenesis in rat kidney cortex (author's transl)].", "content": "Oxygen consumption (QO2), respiratory quotient (RQ) and gluconeogenesis of rat kidney cortex slices were determined (Warburg technique). QO2 was not influenced by 0.01 mM CdCl2 in the incubation medium (no exogenous substrate added), 0.1 mM, 1mM and 10 mM, respectively, reduced QO2 by 43%, 45% and 68%. In the presence of 8 mM glucose, 10 mM acetate or 5 mM butyrate, 1 mM Cd resulted in a decrease in QO2 by about 50% each. Addition of 20 g/l albumine to the medium abolished this effect of cadmium. RQ was not influenced by a mM Cd, both without and with 8 mM glucose in the medium. Glucose production from 7 mM pyruvate or 5 mM glutaminate in slices from fed and starving rats was reduced by 1 mM Cd to half the value found in controls.", "contents": "[On the effect of cadmium on tissue respiration and gluconeogenesis in rat kidney cortex (author's transl)]. Oxygen consumption (QO2), respiratory quotient (RQ) and gluconeogenesis of rat kidney cortex slices were determined (Warburg technique). QO2 was not influenced by 0.01 mM CdCl2 in the incubation medium (no exogenous substrate added), 0.1 mM, 1mM and 10 mM, respectively, reduced QO2 by 43%, 45% and 68%. In the presence of 8 mM glucose, 10 mM acetate or 5 mM butyrate, 1 mM Cd resulted in a decrease in QO2 by about 50% each. Addition of 20 g/l albumine to the medium abolished this effect of cadmium. RQ was not influenced by a mM Cd, both without and with 8 mM glucose in the medium. Glucose production from 7 mM pyruvate or 5 mM glutaminate in slices from fed and starving rats was reduced by 1 mM Cd to half the value found in controls."}
{"id": "PMID:475609", "title": "[Hernia hiatal. Detection of gastroesophageal reflux with the telemetric Heidelberg capsule].", "content": "120 patients with upper gastrointestinal symptoms were studied and submitted to gastric secretory tests, upper gastrointestinal X-rays and endoscopy. Gastroesophageal reflux was assessed by means of a Heidelberg telemetric capsule. 60 patients had hiatal hernia. Gastroesophageal reflux was present in 63.33%. 76% of the patients with hiatal hernia had gastroesophageal reflux, especially on those with small hernias and on the 5th and 6th decade of life. Gastric hiperacidity facilitates the incidence and presence of reflux. X-rays and endoscopy allowed us to diagnose diseases at the gastroesophageal junction. The correlation between these 2 methods and the presence or not of reflux, allowed differentiation between patients with organic diseases and patients with \"functional\" symptoms.", "contents": "[Hernia hiatal. Detection of gastroesophageal reflux with the telemetric Heidelberg capsule]. 120 patients with upper gastrointestinal symptoms were studied and submitted to gastric secretory tests, upper gastrointestinal X-rays and endoscopy. Gastroesophageal reflux was assessed by means of a Heidelberg telemetric capsule. 60 patients had hiatal hernia. Gastroesophageal reflux was present in 63.33%. 76% of the patients with hiatal hernia had gastroesophageal reflux, especially on those with small hernias and on the 5th and 6th decade of life. Gastric hiperacidity facilitates the incidence and presence of reflux. X-rays and endoscopy allowed us to diagnose diseases at the gastroesophageal junction. The correlation between these 2 methods and the presence or not of reflux, allowed differentiation between patients with organic diseases and patients with \"functional\" symptoms."}
{"id": "PMID:475610", "title": "Radiological routine examination of the stomach and duodenum by means of double contrast method.", "content": "The double contrast radiologic examination performed routinely allows us to improve the study of the mucosal relief, thus increasing the diagnosis of gastric ulcers, polyps and gastric cancer. In the operated stomach one can obtain better results with this technique than with the conventional examinations.", "contents": "Radiological routine examination of the stomach and duodenum by means of double contrast method. The double contrast radiologic examination performed routinely allows us to improve the study of the mucosal relief, thus increasing the diagnosis of gastric ulcers, polyps and gastric cancer. In the operated stomach one can obtain better results with this technique than with the conventional examinations."}
{"id": "PMID:475612", "title": "Serum immunoglobulins in patients with schistosomiasis mansoni.", "content": "The authors evaluated immunglobulins IgA, IgC and IgM in two groups of schistosomiasis patients, 20 presenting the hepato-intestinal form (HI) and 20 the hepatosplenic form (HS). The results indicated abnormalities in the following proportions: IgA: HI = 55% HS = 45% IgC: HI = 85% HS = 95% IgM: HI = 75% HS = 85% The authors compare the values of each immunoglobulin in the two groups of patients and evaluate their results.", "contents": "Serum immunoglobulins in patients with schistosomiasis mansoni. The authors evaluated immunglobulins IgA, IgC and IgM in two groups of schistosomiasis patients, 20 presenting the hepato-intestinal form (HI) and 20 the hepatosplenic form (HS). The results indicated abnormalities in the following proportions: IgA: HI = 55% HS = 45% IgC: HI = 85% HS = 95% IgM: HI = 75% HS = 85% The authors compare the values of each immunoglobulin in the two groups of patients and evaluate their results."}
{"id": "PMID:475611", "title": "[South American blastomycosis. Diagnosis by colonoscopy].", "content": "Two cases of South American Blastomycosis are presented; the disease is not frequent in our country; the cases are a segmentary and difuse type primitive localized in the colon. In both of them the colonoscopy was a very important aid in the diagnosis.", "contents": "[South American blastomycosis. Diagnosis by colonoscopy]. Two cases of South American Blastomycosis are presented; the disease is not frequent in our country; the cases are a segmentary and difuse type primitive localized in the colon. In both of them the colonoscopy was a very important aid in the diagnosis."}
{"id": "PMID:475614", "title": "[Salivary amylase in kwashiorkor. III. Investigative study of salivary concentration and secretion in 4 patients presenting complications during nutritional recovery].", "content": "In four kwashiorkor children with ages between 20 and 36 months, the salivary volume (ml), the amylase concentration (U/ml) and secretion (U/Kg/5 min.) was studied, since the first day in the hospital until the discharge. These children had same diseases during the nutritional recuperation. The control group had eighteen children of the same age and eutrophic. The salivary secretion during 5 minutes was taken by special technic and in the same moment in each day. In one patient with serious kwashiorkor the level of the salivary secretion was slow while the nutritional state was bad. The levels of the salivary amylase secretion and concentration was slow while the disease was present making difficult the nutritional recuperation in each patient.", "contents": "[Salivary amylase in kwashiorkor. III. Investigative study of salivary concentration and secretion in 4 patients presenting complications during nutritional recovery]. In four kwashiorkor children with ages between 20 and 36 months, the salivary volume (ml), the amylase concentration (U/ml) and secretion (U/Kg/5 min.) was studied, since the first day in the hospital until the discharge. These children had same diseases during the nutritional recuperation. The control group had eighteen children of the same age and eutrophic. The salivary secretion during 5 minutes was taken by special technic and in the same moment in each day. In one patient with serious kwashiorkor the level of the salivary secretion was slow while the nutritional state was bad. The levels of the salivary amylase secretion and concentration was slow while the disease was present making difficult the nutritional recuperation in each patient."}
{"id": "PMID:475613", "title": "[Articular manifestations of intestinal diseases].", "content": "The articular manifestations of the intestinal diseases can be quite variable affecting primarily either the pripheral joints, or sacroiliac areas and the spine. The presence of the haplotype HLA-B27 is associated in the majority of cases with the presence of spinal involvement. The treatment of the arthritis does not usually follow the natural history of the inflammatory bowel disease.", "contents": "[Articular manifestations of intestinal diseases]. The articular manifestations of the intestinal diseases can be quite variable affecting primarily either the pripheral joints, or sacroiliac areas and the spine. The presence of the haplotype HLA-B27 is associated in the majority of cases with the presence of spinal involvement. The treatment of the arthritis does not usually follow the natural history of the inflammatory bowel disease."}
{"id": "PMID:475618", "title": "Familial tapetoretinal degeneration and epilepsy.", "content": "Tapetoretinal degeneration is described in two siblings in association with generalized major motor seizures and intellectual impairment. Neither of these patients have the characteristic dysmorphic features or biochemical abnormalities seen in previously described cases, which are reviewed. Inheritance was probably autosomal recessive.", "contents": "Familial tapetoretinal degeneration and epilepsy. Tapetoretinal degeneration is described in two siblings in association with generalized major motor seizures and intellectual impairment. Neither of these patients have the characteristic dysmorphic features or biochemical abnormalities seen in previously described cases, which are reviewed. Inheritance was probably autosomal recessive."}
{"id": "PMID:475619", "title": "Histologic findings in motor neuron disease. Relation to clinically determined activity, duration, and severity of disease.", "content": "Correlation of 18 histologic variables with age and sex of 24 patients with motor neuron disease (MND), and the duration, severity, and activity of their disease, showed that high density of atrophic fibers correlated with degree of muscle weakness and the worst prognosis and that type I grouping correlated with the best prognosis. Although both type I and type II fibers are involved in the majority of patients with MND, the data suggest that involvement of type I fibers is more important in relation to activity of the disease.", "contents": "Histologic findings in motor neuron disease. Relation to clinically determined activity, duration, and severity of disease. Correlation of 18 histologic variables with age and sex of 24 patients with motor neuron disease (MND), and the duration, severity, and activity of their disease, showed that high density of atrophic fibers correlated with degree of muscle weakness and the worst prognosis and that type I grouping correlated with the best prognosis. Although both type I and type II fibers are involved in the majority of patients with MND, the data suggest that involvement of type I fibers is more important in relation to activity of the disease."}
{"id": "PMID:475620", "title": "Chiasmal syndrome due to metastasis.", "content": "We examined three patients with a chiasmal syndrome due to metastasis and were able to find only two additional cases in the literature. The characteristic presentation consisted of progressive impairment of vision without headache or abnormal plain skull x-ray films in patients with weight loss or diabetes insipidus. In three of the five patients, the primary malignant neoplasm was occult when symptoms and signs of chiasmal dysfunction were seen. Four patients responded well to irradiation. The chiasm may be compromised by suprasellar extension of pituitary metastasis, by infiltration from an infundicular or hypothalamic metastasis, or by hematogenous spread of tumor.", "contents": "Chiasmal syndrome due to metastasis. We examined three patients with a chiasmal syndrome due to metastasis and were able to find only two additional cases in the literature. The characteristic presentation consisted of progressive impairment of vision without headache or abnormal plain skull x-ray films in patients with weight loss or diabetes insipidus. In three of the five patients, the primary malignant neoplasm was occult when symptoms and signs of chiasmal dysfunction were seen. Four patients responded well to irradiation. The chiasm may be compromised by suprasellar extension of pituitary metastasis, by infiltration from an infundicular or hypothalamic metastasis, or by hematogenous spread of tumor."}
{"id": "PMID:475621", "title": "Familial periodic ataxia.", "content": "Two members of a family suffer from recurrent attacks of either vertigo or cerebellar incoordination. The occurrence of these two distinct types of attack in the same patient may reconcile the disparate features of previously reported cases of familial periodic ataxia. The finding of downbeating nystagmus suggests a medullary disturbance. As in a previously reported family, the attacks were relieved by acetazolamide.", "contents": "Familial periodic ataxia. Two members of a family suffer from recurrent attacks of either vertigo or cerebellar incoordination. The occurrence of these two distinct types of attack in the same patient may reconcile the disparate features of previously reported cases of familial periodic ataxia. The finding of downbeating nystagmus suggests a medullary disturbance. As in a previously reported family, the attacks were relieved by acetazolamide."}
{"id": "PMID:475622", "title": "Eaton-Lambert syndrome in a 9-year-old girl.", "content": "A girl with Eaton-Lambert syndrome had symptoms beginning at the age of 9 years. An extensive search for underlying neoplasm or definable autoimmune disease gave negative findings.", "contents": "Eaton-Lambert syndrome in a 9-year-old girl. A girl with Eaton-Lambert syndrome had symptoms beginning at the age of 9 years. An extensive search for underlying neoplasm or definable autoimmune disease gave negative findings."}
{"id": "PMID:475616", "title": "[Angiographic anatomy of the cecal artery].", "content": "After studying the angiographic anatomy of the cecal artery in 80 post--mortem examinations we have demonstrated the following: -The common cecal trunk (76,2% of the cases) had its origin from the right colic artery in 12 (15%) and from the ileal artery in 49 (61,2%) observations. -The anterior and posterior cecal arteries had a separate origin in 19 observations (23,7%). In 7 of these observations (8,7%) the anterior cecal artery had its origin from the right colic artery and, the posterior cecal artery originated directly from the ileal artery. In 11 observations (13,8%) both cecal arteries, originated directly from the ileal artery and, in one case (1,2%) the anterior cecal artery originated from the right colic artery and the posterior cecal artery from the ileocolic artery.", "contents": "[Angiographic anatomy of the cecal artery]. After studying the angiographic anatomy of the cecal artery in 80 post--mortem examinations we have demonstrated the following: -The common cecal trunk (76,2% of the cases) had its origin from the right colic artery in 12 (15%) and from the ileal artery in 49 (61,2%) observations. -The anterior and posterior cecal arteries had a separate origin in 19 observations (23,7%). In 7 of these observations (8,7%) the anterior cecal artery had its origin from the right colic artery and, the posterior cecal artery originated directly from the ileal artery. In 11 observations (13,8%) both cecal arteries, originated directly from the ileal artery and, in one case (1,2%) the anterior cecal artery originated from the right colic artery and the posterior cecal artery from the ileocolic artery."}
{"id": "PMID:475623", "title": "Micromyoclonic seizures of sleep.", "content": "A patient with primary generalized epilepsy had convulsive seizures refractory to anticonvulsant therapy. Several waking EEGs failed to show paroxysmal activity, but a sleep recording revealed polyspike EEG discharges associated with individual electromyographic discharges localized to the chin. The discharges occurred with a 90- to 100-minute period during the night. An increased frequency and duration of the EEG discharges on the last night of recording preceded a marked increase in the number of convulsions the following day.", "contents": "Micromyoclonic seizures of sleep. A patient with primary generalized epilepsy had convulsive seizures refractory to anticonvulsant therapy. Several waking EEGs failed to show paroxysmal activity, but a sleep recording revealed polyspike EEG discharges associated with individual electromyographic discharges localized to the chin. The discharges occurred with a 90- to 100-minute period during the night. An increased frequency and duration of the EEG discharges on the last night of recording preceded a marked increase in the number of convulsions the following day."}
{"id": "PMID:475624", "title": "Value of second lumbar puncture in confirming a diagnosis of aseptic meningitis. A prospective study.", "content": "In patients with viral meningitis, polymorphonuclear leukocytes sometimes predominate in the CSF on initial examination. In a prospective analysis of this phenomenon, 16 consecutive patients with viral meningitis were followed up with serial CSF studies. The percentage of polymorphonuclear leukocytes showed a significant fall between initial and second examinations (41.75 +/- 27.0 to 8.6 +/- 8.78 [mean +/- 2 SD], P less than .001), while total WBC counts and the protein and sugar content levels remained unchanged. Based mainly upon this \"polymorph shift,\" antibiotic therapy was correctly withheld from 100% of patients reexamined. On subsequent examinations, the percentage of polymorphonuclear cells remained low. All patients recovered completely without any specific treatment. In otherwise healthy subjects with the aseptic meningitis syndrome, antibiotic therapy can be withheld even when polymorphonuclear cells predominate on initial CSF examination. If suspicion arises regarding the diagnosis, another examination will demonstrate a significant fall in polymorphonuclear cells if the initial impression was correct.", "contents": "Value of second lumbar puncture in confirming a diagnosis of aseptic meningitis. A prospective study. In patients with viral meningitis, polymorphonuclear leukocytes sometimes predominate in the CSF on initial examination. In a prospective analysis of this phenomenon, 16 consecutive patients with viral meningitis were followed up with serial CSF studies. The percentage of polymorphonuclear leukocytes showed a significant fall between initial and second examinations (41.75 +/- 27.0 to 8.6 +/- 8.78 [mean +/- 2 SD], P less than .001), while total WBC counts and the protein and sugar content levels remained unchanged. Based mainly upon this \"polymorph shift,\" antibiotic therapy was correctly withheld from 100% of patients reexamined. On subsequent examinations, the percentage of polymorphonuclear cells remained low. All patients recovered completely without any specific treatment. In otherwise healthy subjects with the aseptic meningitis syndrome, antibiotic therapy can be withheld even when polymorphonuclear cells predominate on initial CSF examination. If suspicion arises regarding the diagnosis, another examination will demonstrate a significant fall in polymorphonuclear cells if the initial impression was correct."}
{"id": "PMID:475625", "title": "Necrotizing myelopathy complicating Hodgkin's disease.", "content": "A case of necrotizing myelopathy is presented as a complication of Hodgkin's disease. The distinctive pathologic feature of this case was severe acute vasculitis involving only the vessels of the spinal cord that was considered to have been the cause of the segmental necrosis.", "contents": "Necrotizing myelopathy complicating Hodgkin's disease. A case of necrotizing myelopathy is presented as a complication of Hodgkin's disease. The distinctive pathologic feature of this case was severe acute vasculitis involving only the vessels of the spinal cord that was considered to have been the cause of the segmental necrosis."}
{"id": "PMID:475626", "title": "Does gastrectomy predispose to amyotrophic lateral sclerosis?", "content": "Some authors have suggested that amyotrophic lateral sclerosis (ALS) may be precipitated by a \"deficiency factor\" following gastrectomy. Methods to establish a statistical association of the disease with gastrectomy include a case-control study. Two such studies have been made in Japan, one involving 712 and the other 158 cases of the diseases. No statistically significant association between gastrectomy and the subsequent occurrence of ALS was found, indicating it is unlikely that the operation predisposes to ALS. None of many other factors studied except mechanical injury showed a statistically significant association in either study.", "contents": "Does gastrectomy predispose to amyotrophic lateral sclerosis? Some authors have suggested that amyotrophic lateral sclerosis (ALS) may be precipitated by a \"deficiency factor\" following gastrectomy. Methods to establish a statistical association of the disease with gastrectomy include a case-control study. Two such studies have been made in Japan, one involving 712 and the other 158 cases of the diseases. No statistically significant association between gastrectomy and the subsequent occurrence of ALS was found, indicating it is unlikely that the operation predisposes to ALS. None of many other factors studied except mechanical injury showed a statistically significant association in either study."}
{"id": "PMID:475627", "title": "Electromyographic abnormalities following myelography.", "content": "Electromyographic (EMG) abnormalities were found in 13 of 32 patients with previously normal studies following myelography. These abnormalities usually developed by the first day after myelography and resolved by the fourth day. The changes were evenly divided between those restricted to a single paraspinal muscle level, thus mimicking the frequent findings in radioculopathy, and those present bilaterally and at multiple levels. We were unable to correlate these EMG changes with the clinical status of the patient or with events occurring at myelography. Thus, the performance of EMG within four days following myelography can lead to false-positive results if paraspinal muscle EMG abnormalities are used as the diagnostic criteria.", "contents": "Electromyographic abnormalities following myelography. Electromyographic (EMG) abnormalities were found in 13 of 32 patients with previously normal studies following myelography. These abnormalities usually developed by the first day after myelography and resolved by the fourth day. The changes were evenly divided between those restricted to a single paraspinal muscle level, thus mimicking the frequent findings in radioculopathy, and those present bilaterally and at multiple levels. We were unable to correlate these EMG changes with the clinical status of the patient or with events occurring at myelography. Thus, the performance of EMG within four days following myelography can lead to false-positive results if paraspinal muscle EMG abnormalities are used as the diagnostic criteria."}
{"id": "PMID:475628", "title": "'Agenesis' of the temporal lobe. A case report with autopsy findings.", "content": "The temporal lobe agenesis syndrome is a rare congenital abnormality. In previous case reports, this syndrome has been described in association with arachnoid cysts or abnormal collections of CSF. An autopsy performed in the case of our 25-year-old patient revealed agenesis of frontal and temporal lobes without an anatomic cyst. During life the patient had no neurologic abnormalities that could be related to the lesion.", "contents": "'Agenesis' of the temporal lobe. A case report with autopsy findings. The temporal lobe agenesis syndrome is a rare congenital abnormality. In previous case reports, this syndrome has been described in association with arachnoid cysts or abnormal collections of CSF. An autopsy performed in the case of our 25-year-old patient revealed agenesis of frontal and temporal lobes without an anatomic cyst. During life the patient had no neurologic abnormalities that could be related to the lesion."}
{"id": "PMID:475629", "title": "Presenile dementia with motor neuron disease in Japan. A new entity?", "content": "A 61-year-old woman suffered the gradual onset of difficulty with memory, concentration, and cognition at age 58. Progressively more severe dementia was accompanied by muscle wasting and fasciculation prominent in hand and bulbar muscles. An electromyogram and a muscle biopsy specimen demonstrated denervation patterns, and a computerized tomographic scan showed considerable cerebral atrophy. This report reviews cases of presenile dementia with motor neuron disease reported in Japan and discusses the possibility of a new clinicopathologic entity.", "contents": "Presenile dementia with motor neuron disease in Japan. A new entity? A 61-year-old woman suffered the gradual onset of difficulty with memory, concentration, and cognition at age 58. Progressively more severe dementia was accompanied by muscle wasting and fasciculation prominent in hand and bulbar muscles. An electromyogram and a muscle biopsy specimen demonstrated denervation patterns, and a computerized tomographic scan showed considerable cerebral atrophy. This report reviews cases of presenile dementia with motor neuron disease reported in Japan and discusses the possibility of a new clinicopathologic entity."}
{"id": "PMID:475632", "title": "Tear osmolarity and ocular surface disease in keratoconjunctivitis sicca.", "content": "Tear osmolarity and Rose Bengal staining were studied in a group of patients with keratoconjunctivitis sicca (KCS) before and after treatment with isotonic and one-half isotonic saline to compare treatment with these two solutions and to ascertain whether tear film osmolarity could be correlated with ocular surface disease. When 20 KCS eyes were treated with isotonic saline drops every three hours for one week, the average (+/-SD) tear osmolarity decreased from 365 +/- 77 mOsm/L to 329 +/- 47 mOsm/L, and the average (+/-SD) Rose Bengal staining score decreased from 4.1 +/- 3.1 to 3.6 +/- 3.3. There was no objective difference between treatment with isotonic and one-half isotonic solutions, in a double-masked comparison, however, four of five patients with diagnostically significant Rose Bengal staining preferred the half isotonic solution. There was a significant positive correlation between tear film osmolarity and Rose Bengal staining.", "contents": "Tear osmolarity and ocular surface disease in keratoconjunctivitis sicca. Tear osmolarity and Rose Bengal staining were studied in a group of patients with keratoconjunctivitis sicca (KCS) before and after treatment with isotonic and one-half isotonic saline to compare treatment with these two solutions and to ascertain whether tear film osmolarity could be correlated with ocular surface disease. When 20 KCS eyes were treated with isotonic saline drops every three hours for one week, the average (+/-SD) tear osmolarity decreased from 365 +/- 77 mOsm/L to 329 +/- 47 mOsm/L, and the average (+/-SD) Rose Bengal staining score decreased from 4.1 +/- 3.1 to 3.6 +/- 3.3. There was no objective difference between treatment with isotonic and one-half isotonic solutions, in a double-masked comparison, however, four of five patients with diagnostically significant Rose Bengal staining preferred the half isotonic solution. There was a significant positive correlation between tear film osmolarity and Rose Bengal staining."}
{"id": "PMID:475633", "title": "Specular microscopy of traumatic posterior annular keratopathy.", "content": "Specular microscopic studies in two patients with traumatic posterior annular keratopathy demonstrated that the endothelial rings consist of disrupted and swollen endothelial cells. The damaged cells were still evident many days after the rings had disappeared. The amount of endothelial cell loss depends on the severity of the injury, and a measurable decrease in cell density occurs only in the more severely injured patients. A physical explanation for the occurrence of these endothelial rings is discussed.", "contents": "Specular microscopy of traumatic posterior annular keratopathy. Specular microscopic studies in two patients with traumatic posterior annular keratopathy demonstrated that the endothelial rings consist of disrupted and swollen endothelial cells. The damaged cells were still evident many days after the rings had disappeared. The amount of endothelial cell loss depends on the severity of the injury, and a measurable decrease in cell density occurs only in the more severely injured patients. A physical explanation for the occurrence of these endothelial rings is discussed."}
{"id": "PMID:475634", "title": "Germinating seed in anterior chamber. Report of an unusual case.", "content": "A seed introduced into the eye of an 8-year-old boy through a penetrating corneal wound became embedded in the iris. Many months later it germinated. The germinating seed was successfully removed from the iris and anterior chamber with retention of excellent visual acuity. The seed was identified as a dicotyledonous seedling of the family Compositae.", "contents": "Germinating seed in anterior chamber. Report of an unusual case. A seed introduced into the eye of an 8-year-old boy through a penetrating corneal wound became embedded in the iris. Many months later it germinated. The germinating seed was successfully removed from the iris and anterior chamber with retention of excellent visual acuity. The seed was identified as a dicotyledonous seedling of the family Compositae."}
{"id": "PMID:475635", "title": "Lacrimal function tests.", "content": "A comparative study of lacrimal function tests was undertaken. The primary Jones fluorescein test, the fluorescein dye disappearance (FDD) test, the Hornblass saccharin taste test and the Schirmer secretory test were tested for their reliability and effectiveness in 60 lacrimal systems with no known pathologic history or physical findings. Schirmer test values diminish with advancing age. The correlation between the FDD test, the Jones fluorescein test, and the saccharin taste test was low. The FDD test is an unsatisfactory test. The saccharin taste test time was shorter in patients with a positive Jones fluorescein test and longer in patients with a negative Jones fluorescein test. Forty-eight percent or 31/60 lacrimal systems had a negative Jones fluorescein test. This is higher than previously reported. The saccharin taste test was found to be the most practical and reliable of all lacrimal excretory function tests despite its subjectivity.", "contents": "Lacrimal function tests. A comparative study of lacrimal function tests was undertaken. The primary Jones fluorescein test, the fluorescein dye disappearance (FDD) test, the Hornblass saccharin taste test and the Schirmer secretory test were tested for their reliability and effectiveness in 60 lacrimal systems with no known pathologic history or physical findings. Schirmer test values diminish with advancing age. The correlation between the FDD test, the Jones fluorescein test, and the saccharin taste test was low. The FDD test is an unsatisfactory test. The saccharin taste test time was shorter in patients with a positive Jones fluorescein test and longer in patients with a negative Jones fluorescein test. Forty-eight percent or 31/60 lacrimal systems had a negative Jones fluorescein test. This is higher than previously reported. The saccharin taste test was found to be the most practical and reliable of all lacrimal excretory function tests despite its subjectivity."}
{"id": "PMID:475636", "title": "Congenital mucocele of the lacrimal sac.", "content": "Two infants had congenital mucocele of the lacrimal sac. The diagnosis of this condition was aided by ultrasound. Congenital mucocele is one of the few indications for immediate lacrimal system probing in the neonatal period. Probing in both patients was successful.", "contents": "Congenital mucocele of the lacrimal sac. Two infants had congenital mucocele of the lacrimal sac. The diagnosis of this condition was aided by ultrasound. Congenital mucocele is one of the few indications for immediate lacrimal system probing in the neonatal period. Probing in both patients was successful."}
{"id": "PMID:475637", "title": "Blepharophimosis, ptosis, epicanthus inversus, and primary amenorrhea. A dominant trait.", "content": "The syndrome of blepharophimosis, ptosis, and epicanthus inversus is a rare but well-documented autosomal dominant disorder. Although autosomal dominant inheritance has been repeatedly confirmed, several observers have noted that transmission of the trait is predominantly through an affected male parent. To our knowledge, no published observations have accounted for this unusual differential transmission. Described here is a family with typical features of this disorder and in addition primary amenorrhea in the affected women. The primary amenorrhea, which has not been previously documented in this disorder, does account for differential transmission in this family and possibly in other families. Since affected individuals are treated by ophthalmologists, the possible occurrence of this extraocular manifestation should be recognized in the management of patients and their families.", "contents": "Blepharophimosis, ptosis, epicanthus inversus, and primary amenorrhea. A dominant trait. The syndrome of blepharophimosis, ptosis, and epicanthus inversus is a rare but well-documented autosomal dominant disorder. Although autosomal dominant inheritance has been repeatedly confirmed, several observers have noted that transmission of the trait is predominantly through an affected male parent. To our knowledge, no published observations have accounted for this unusual differential transmission. Described here is a family with typical features of this disorder and in addition primary amenorrhea in the affected women. The primary amenorrhea, which has not been previously documented in this disorder, does account for differential transmission in this family and possibly in other families. Since affected individuals are treated by ophthalmologists, the possible occurrence of this extraocular manifestation should be recognized in the management of patients and their families."}
{"id": "PMID:475638", "title": "Pigmentary dispersion and glaucoma. A new theory.", "content": "A new theory states that mechanical rubbing between anterior packets of zonules and the peripheral iris in predisposed eyes is the cause of the loss of iris pigment in pigmentary glaucoma and in the pigmentary dispersion syndrome. The peripheral radial defects were in close approximation to anterior packets. The total number of iris defects in advanced cases correlated with the average number of anterior packets. Histologic study showed a groove in the stroma of the iris as well as loss of the pigmented neuroepithelium and was compatible with mechanical damage. The theory explains why the disease predominates in young myopic men, why it decreases in incidence with age, and why, in some cases, it regresses. Treatment with an alpha-adrenergic blocking agent may be advantageous in this condition.", "contents": "Pigmentary dispersion and glaucoma. A new theory. A new theory states that mechanical rubbing between anterior packets of zonules and the peripheral iris in predisposed eyes is the cause of the loss of iris pigment in pigmentary glaucoma and in the pigmentary dispersion syndrome. The peripheral radial defects were in close approximation to anterior packets. The total number of iris defects in advanced cases correlated with the average number of anterior packets. Histologic study showed a groove in the stroma of the iris as well as loss of the pigmented neuroepithelium and was compatible with mechanical damage. The theory explains why the disease predominates in young myopic men, why it decreases in incidence with age, and why, in some cases, it regresses. Treatment with an alpha-adrenergic blocking agent may be advantageous in this condition."}
{"id": "PMID:475639", "title": "Saccadic intrusions in strabismus.", "content": "Fixational eye movements were studied to determine the presence of and to quantify saccadic intrusions under monocular and binocular viewing conditions in subjects with intermittent strabismus, amblyopia without stabismus, or constant strabismus amblyopia. Saccadic intrusions were present under most test conditions in intermittent strabismus, were rarely observed in amblyopia without strabismus, and were prominent during monocular fixation with the amblyopic eye in constant strabismus anblyopia. This suggests that the presence of saccadic intrusions was related to strabismus and not amblyopia. There was no relationship between saccadic intrusion amplitude and visual acuity. Two possible mechanisms for producing intrusions are abnormally rapid regional visual adaptation and strabismus-induced fixation degradation.", "contents": "Saccadic intrusions in strabismus. Fixational eye movements were studied to determine the presence of and to quantify saccadic intrusions under monocular and binocular viewing conditions in subjects with intermittent strabismus, amblyopia without stabismus, or constant strabismus amblyopia. Saccadic intrusions were present under most test conditions in intermittent strabismus, were rarely observed in amblyopia without strabismus, and were prominent during monocular fixation with the amblyopic eye in constant strabismus anblyopia. This suggests that the presence of saccadic intrusions was related to strabismus and not amblyopia. There was no relationship between saccadic intrusion amplitude and visual acuity. Two possible mechanisms for producing intrusions are abnormally rapid regional visual adaptation and strabismus-induced fixation degradation."}
{"id": "PMID:475640", "title": "Balloon cell melanomas of the ciliary body.", "content": "In spite of the fact that balloon cell change has been recognized ophthalmoscopically and histologically in certain posterior choroidal melanomas, the nature and importance of this observation remain controversial. This article provides a clinicopathologic correlation in two cases of ciliary body melanoma with almost total balloon cell transformation. On the basis of special stains and electron microscopic observations, we have concluded that balloon cells in such cases represent spindle melanoma cells that have undergoing extensive cytoplasmic lipid metamorphosis. The possible mechanisms for this change are considered. Melanomas containing an abundant number of balloon cells are probably comparatively dormant and benign tumors that offer a relatively good prognosis. Although clinical recognition may be difficult, the use of needle biopsy coupled with cytologic diagnosis in selected ciliary body tumors may disclose balloon cell change and help to direct therapy.", "contents": "Balloon cell melanomas of the ciliary body. In spite of the fact that balloon cell change has been recognized ophthalmoscopically and histologically in certain posterior choroidal melanomas, the nature and importance of this observation remain controversial. This article provides a clinicopathologic correlation in two cases of ciliary body melanoma with almost total balloon cell transformation. On the basis of special stains and electron microscopic observations, we have concluded that balloon cells in such cases represent spindle melanoma cells that have undergoing extensive cytoplasmic lipid metamorphosis. The possible mechanisms for this change are considered. Melanomas containing an abundant number of balloon cells are probably comparatively dormant and benign tumors that offer a relatively good prognosis. Although clinical recognition may be difficult, the use of needle biopsy coupled with cytologic diagnosis in selected ciliary body tumors may disclose balloon cell change and help to direct therapy."}
{"id": "PMID:475641", "title": "Intraocular penetration of miconazole in rabbits.", "content": "Data from this in vivo albino rabbit study suggest that miconazole nitrate may penetrate the ocular compartments better than either natamycin or amphotericin B after intravenous, subconjunctival, or topical administration. The concentrations of miconazole in cornea and in aqueous humor after either topical or subconjunctival administration were very high, and a further threefold increase in the levels was seen if the corneal epithelium had been removed prior to drug therapy. Miconazole was found in the vitreous in some animals after subconjunctival injections of the drug. Intravenous administration produced high concentrations of miconazole in the aqueous humor, which rapidly fell over eight hours. No signs of toxicity or adverse reactions were found in these short-term experiments. Miconazole may be a useful addition in our methods of treating keratomycosis and oculomycosis.", "contents": "Intraocular penetration of miconazole in rabbits. Data from this in vivo albino rabbit study suggest that miconazole nitrate may penetrate the ocular compartments better than either natamycin or amphotericin B after intravenous, subconjunctival, or topical administration. The concentrations of miconazole in cornea and in aqueous humor after either topical or subconjunctival administration were very high, and a further threefold increase in the levels was seen if the corneal epithelium had been removed prior to drug therapy. Miconazole was found in the vitreous in some animals after subconjunctival injections of the drug. Intravenous administration produced high concentrations of miconazole in the aqueous humor, which rapidly fell over eight hours. No signs of toxicity or adverse reactions were found in these short-term experiments. Miconazole may be a useful addition in our methods of treating keratomycosis and oculomycosis."}
{"id": "PMID:475642", "title": "Experimental vitreous fibroplasia following perforating ocular injuries.", "content": "Biochemical data and histopathologic observations were made on an experimental model of vitreous fibroplasia in the rabbit eye, with comparisons between the change in vitreous collagen concentration and histologic characteristics 7, 14, and 30 days after injury. Prior to two weeks after injury there is a relatively small amount of new vitreous collagen formed, associated with an inflammatory and vascular reaction. At four weeks there is a marked increase in new vitreous collagen and dense fibrous sheets are noted. Vitreous hemorrhage accelerates collagen formation, and methylprednisolone sodium succinate does not decrease vitreous fibrosis. Our results suggest that the optimum time for vitrectomy, under the conditions of this experiment, is two weeks after primary repair. The efficacy of steroids in retarding vitreous fibroplasia is not supported.", "contents": "Experimental vitreous fibroplasia following perforating ocular injuries. Biochemical data and histopathologic observations were made on an experimental model of vitreous fibroplasia in the rabbit eye, with comparisons between the change in vitreous collagen concentration and histologic characteristics 7, 14, and 30 days after injury. Prior to two weeks after injury there is a relatively small amount of new vitreous collagen formed, associated with an inflammatory and vascular reaction. At four weeks there is a marked increase in new vitreous collagen and dense fibrous sheets are noted. Vitreous hemorrhage accelerates collagen formation, and methylprednisolone sodium succinate does not decrease vitreous fibrosis. Our results suggest that the optimum time for vitrectomy, under the conditions of this experiment, is two weeks after primary repair. The efficacy of steroids in retarding vitreous fibroplasia is not supported."}
{"id": "PMID:475643", "title": "Vitreous inhibition of tumor neovascularization.", "content": "Cartilage has been reported to contain a factor that inhibits tumor neovascularization. Rabbit vitreous, another avascular tissue, has also been shown recently to have an inhibitory effect on tumor neovascularization. This report describes our results on the effects of bovine and human vitreous on tumor neovascularization with use of a rabbit cornea model. Inhibition of neovascularization was observed with both bovine and human vitreous in the form of reduced new blood vessel growth over and around the vitreous pellet and subsequent delayed exophytic tumor growth. Stimulation of neovascularization by bovine and human vitreous was also observed as an early, short-lived response.", "contents": "Vitreous inhibition of tumor neovascularization. Cartilage has been reported to contain a factor that inhibits tumor neovascularization. Rabbit vitreous, another avascular tissue, has also been shown recently to have an inhibitory effect on tumor neovascularization. This report describes our results on the effects of bovine and human vitreous on tumor neovascularization with use of a rabbit cornea model. Inhibition of neovascularization was observed with both bovine and human vitreous in the form of reduced new blood vessel growth over and around the vitreous pellet and subsequent delayed exophytic tumor growth. Stimulation of neovascularization by bovine and human vitreous was also observed as an early, short-lived response."}
{"id": "PMID:475644", "title": "Clinical specular microscopy. I. Optical principles.", "content": "The clinical specular microscope yields a corneal endothelial image. Depending on the slit width of the illumination source, a typical endothelial photomicrograph contains three or four distinct zones. The appearance of the boundary between the endothelial cell pattern and the adjacent dark zone, called the dark boundary, reflects the configuration of the endothelial cell-aqueous humor interface and provides important information about the posterior corneal surface.", "contents": "Clinical specular microscopy. I. Optical principles. The clinical specular microscope yields a corneal endothelial image. Depending on the slit width of the illumination source, a typical endothelial photomicrograph contains three or four distinct zones. The appearance of the boundary between the endothelial cell pattern and the adjacent dark zone, called the dark boundary, reflects the configuration of the endothelial cell-aqueous humor interface and provides important information about the posterior corneal surface."}
{"id": "PMID:475645", "title": "Clinical specular microscopy. II. Qualitative evaluation of corneal endothelial photomicrographs.", "content": "The clinical specular microscope shows the morphological appearance of the endothelium in normal and abnormal corneas. This instrument resolves the endothelial mosaic of the normal cornea into a quasiregular pattern of contiguous cells having well-defined cell boundaries. Cell size varies over a wide range in a number of disorders, and endothelial cells may assume shapes that are substantially different from their usual hexagonal appearance. Cell boundaries are dark and most commonly appear as a straight, narrow line. However, other types of cell boundaries, collectively referred to as doubled boundaries, have been encountered. Cell boundaries normally intersect in a manner that results in three angles of intersection, each approximately 60 degrees, but variations from this pattern are seen. A number of noncellular structures also can be seen in the endothelial zone.", "contents": "Clinical specular microscopy. II. Qualitative evaluation of corneal endothelial photomicrographs. The clinical specular microscope shows the morphological appearance of the endothelium in normal and abnormal corneas. This instrument resolves the endothelial mosaic of the normal cornea into a quasiregular pattern of contiguous cells having well-defined cell boundaries. Cell size varies over a wide range in a number of disorders, and endothelial cells may assume shapes that are substantially different from their usual hexagonal appearance. Cell boundaries are dark and most commonly appear as a straight, narrow line. However, other types of cell boundaries, collectively referred to as doubled boundaries, have been encountered. Cell boundaries normally intersect in a manner that results in three angles of intersection, each approximately 60 degrees, but variations from this pattern are seen. A number of noncellular structures also can be seen in the endothelial zone."}
{"id": "PMID:475646", "title": "Auditory potentials of cochlear nerve and brain stem in multiple sclerosis.", "content": "Auditory evoked potentials of the cochlear nerve and the brain stem were recorded in ten patients with multiple sclerosis (MS) and four patients thought to possibly have MS. A characteristic pattern materialized, with a prolonged N1-P4 conduction time and absent or small N2 and/or N5 waves. We discuss possible clinical implications.", "contents": "Auditory potentials of cochlear nerve and brain stem in multiple sclerosis. Auditory evoked potentials of the cochlear nerve and the brain stem were recorded in ten patients with multiple sclerosis (MS) and four patients thought to possibly have MS. A characteristic pattern materialized, with a prolonged N1-P4 conduction time and absent or small N2 and/or N5 waves. We discuss possible clinical implications."}
{"id": "PMID:475647", "title": "Delineating the cleft in upper blepharoplasty.", "content": "The subject of the esthetic qualities of the upper eyelid-eyebrow complex is discussed and how the cosmetic surgeon should diagnostically evaluate the problems occurring in this area. Emphasis is put on the other contributing factors that may contribute to blepharochalasis of the upper lid other than redundant skin limited to the upper lid space. A blepharoplasty technique with an incision at the supratarsal fold and evaluation of a skin muscle flap is discussed briefly.", "contents": "Delineating the cleft in upper blepharoplasty. The subject of the esthetic qualities of the upper eyelid-eyebrow complex is discussed and how the cosmetic surgeon should diagnostically evaluate the problems occurring in this area. Emphasis is put on the other contributing factors that may contribute to blepharochalasis of the upper lid other than redundant skin limited to the upper lid space. A blepharoplasty technique with an incision at the supratarsal fold and evaluation of a skin muscle flap is discussed briefly."}
{"id": "PMID:475648", "title": "Hearing aid selection. An analysis and point of view.", "content": "Hearing aid selection and hearing aid evaluation are related, but separate, processes. Adequate selection must precede evaluation, which can only be applied to a few of the limitless amplification possibilities. I discuss the theoretical principles of aid selection and illustrate their application by reference to aid selection procedures developed in the National Acoustic Laboratories, Australia. Unless selection is arbitrary, a theoretical method is being used even if not recognized or applied consistently. It is argued that casual methods cannot be adequate and should be replaced by the adoption of theoretical procedures that are explicit, systematic, comprehensive, and subject to critical examination and refinement.", "contents": "Hearing aid selection. An analysis and point of view. Hearing aid selection and hearing aid evaluation are related, but separate, processes. Adequate selection must precede evaluation, which can only be applied to a few of the limitless amplification possibilities. I discuss the theoretical principles of aid selection and illustrate their application by reference to aid selection procedures developed in the National Acoustic Laboratories, Australia. Unless selection is arbitrary, a theoretical method is being used even if not recognized or applied consistently. It is argued that casual methods cannot be adequate and should be replaced by the adoption of theoretical procedures that are explicit, systematic, comprehensive, and subject to critical examination and refinement."}
{"id": "PMID:475649", "title": "The retroconchal space of the lateral nasal wall.", "content": "The retroconchal space is defined in this article as the posterior part of the lateral wall of the nasal fossa. Considerable variations in the size and shape of this retroconchal space exist, these in turn depending on the variations of the middle and inferior conchae. The retroconchal space can be defined in plain radiographs or in nasopharyngograms. This would be of help in pinpointing the site of a neoplasm.", "contents": "The retroconchal space of the lateral nasal wall. The retroconchal space is defined in this article as the posterior part of the lateral wall of the nasal fossa. Considerable variations in the size and shape of this retroconchal space exist, these in turn depending on the variations of the middle and inferior conchae. The retroconchal space can be defined in plain radiographs or in nasopharyngograms. This would be of help in pinpointing the site of a neoplasm."}
{"id": "PMID:475650", "title": "Transmastoid decompression of the facial nerve in Bell's palsy.", "content": "We describe the technique of decompression of the facial nerve from the geniculate ganglion to the stylomastoid foramen. The decompression was made on 51 patients with Bell's palsy, and the results of follow-up study were compared with those of 77 patients in whom the pyramidal and vertical segments were decompressed. The decompressions were indicated when a severe denervation of the nerve was confirmed by electrodiagnostic examinations. Incidences of notable residual palsy and of pronounced sequelae were always lower in the former group of patients regardless of the operational timing. In either group, a more desirable result was obtained by the decompression within 30 days, but a favorable effect of the decompression was recognizable even when the surgery was performed between 31 and 90 days after the onset.", "contents": "Transmastoid decompression of the facial nerve in Bell's palsy. We describe the technique of decompression of the facial nerve from the geniculate ganglion to the stylomastoid foramen. The decompression was made on 51 patients with Bell's palsy, and the results of follow-up study were compared with those of 77 patients in whom the pyramidal and vertical segments were decompressed. The decompressions were indicated when a severe denervation of the nerve was confirmed by electrodiagnostic examinations. Incidences of notable residual palsy and of pronounced sequelae were always lower in the former group of patients regardless of the operational timing. In either group, a more desirable result was obtained by the decompression within 30 days, but a favorable effect of the decompression was recognizable even when the surgery was performed between 31 and 90 days after the onset."}
{"id": "PMID:475651", "title": "Surgical management of drooling.", "content": "The presence of drooling is an indication of an upset in the coordinated mechanism of facial, tongue, and palate muscles. This upset is particularly common in children with cerebral palsy. After extensive investigation and the establishment of the relative significance of the drooling and the degree of cerebral palsy, positive treatment with physiotherapy is then commenced. Those patients whose conditions fail to improve adequately with positive physiotherapy can be helped by staging surgery. Surgery treatment is carried out as a planned procedure. The submandibular ducts are transposed. Submucosal dissection and redirection of salivary flow from the submandibular glands excludes the necessity for extirpation of the salivary glands.", "contents": "Surgical management of drooling. The presence of drooling is an indication of an upset in the coordinated mechanism of facial, tongue, and palate muscles. This upset is particularly common in children with cerebral palsy. After extensive investigation and the establishment of the relative significance of the drooling and the degree of cerebral palsy, positive treatment with physiotherapy is then commenced. Those patients whose conditions fail to improve adequately with positive physiotherapy can be helped by staging surgery. Surgery treatment is carried out as a planned procedure. The submandibular ducts are transposed. Submucosal dissection and redirection of salivary flow from the submandibular glands excludes the necessity for extirpation of the salivary glands."}
{"id": "PMID:475652", "title": "Effect of urea on osmolality of perilymph.", "content": "Blood osmolality was altered in chinchillas by intravenous administration of urea. Serum osmolality peaked rapidly at 30 minutes after administration and decreased slightly to a plateau for 180 minutes. Perilymph and CSF osmolality lagged substantially behind the increase in serum osmolality and equaled serum osmolality only after one hour. Perilymph osmolality followed changes in the serum up to 60 minutes with a definite time lag. This phenomenon suggests the existence of a selective blood-labyrinth barrier that is permeable to urea and water. However, the time lag due to the barrier may permit the reduction of hydrostatic pressure in the labyrinth. The results of the present study seem to render partial explanation of improved hearing in patients with Meniere's disease who were treated with urea.", "contents": "Effect of urea on osmolality of perilymph. Blood osmolality was altered in chinchillas by intravenous administration of urea. Serum osmolality peaked rapidly at 30 minutes after administration and decreased slightly to a plateau for 180 minutes. Perilymph and CSF osmolality lagged substantially behind the increase in serum osmolality and equaled serum osmolality only after one hour. Perilymph osmolality followed changes in the serum up to 60 minutes with a definite time lag. This phenomenon suggests the existence of a selective blood-labyrinth barrier that is permeable to urea and water. However, the time lag due to the barrier may permit the reduction of hydrostatic pressure in the labyrinth. The results of the present study seem to render partial explanation of improved hearing in patients with Meniere's disease who were treated with urea."}
{"id": "PMID:475653", "title": "Clinical evaluation of nasal obstruction. A study of 1,000 patients.", "content": "Nasal airway resistance was evaluated in 1,000 patients who were seen at the Mayo Clinic between 1971 and 1977 for rhinologic complaints. Nasal obstruction was reported as the major symptom in 863 patients. Nasal airway resistance correlated well with the side and the severity of the obstructive symptoms. Symptoms of nasal obstruction typically occurred with a total nasal resistance greater than 3 cm H2O/L/s. Rhinoscopic evidence of nasal deformity could not be used to predict an increase in nasal resistance since many patients with rhinoscopically abnormal noses had \"normal\" nasal resistances. We conclude that the calculation of nasal airway resistance is a useful technique in the clinical evaluation of nasal obstruction. Nasal airway resistance data provide objective values that can substantiate and quantitate the symptoms of nasal obstruction.", "contents": "Clinical evaluation of nasal obstruction. A study of 1,000 patients. Nasal airway resistance was evaluated in 1,000 patients who were seen at the Mayo Clinic between 1971 and 1977 for rhinologic complaints. Nasal obstruction was reported as the major symptom in 863 patients. Nasal airway resistance correlated well with the side and the severity of the obstructive symptoms. Symptoms of nasal obstruction typically occurred with a total nasal resistance greater than 3 cm H2O/L/s. Rhinoscopic evidence of nasal deformity could not be used to predict an increase in nasal resistance since many patients with rhinoscopically abnormal noses had \"normal\" nasal resistances. We conclude that the calculation of nasal airway resistance is a useful technique in the clinical evaluation of nasal obstruction. Nasal airway resistance data provide objective values that can substantiate and quantitate the symptoms of nasal obstruction."}
{"id": "PMID:475654", "title": "Osteonecrosis of hyoid bone and thyroid cartilage.", "content": "A 55-year-old man with carcinoma of the right pyriform fossa was treated with cobalt therapy. Subsequently, osteonecrosis of the right greater horn and the right superior horn of the thyroid cartilage developed, followed by pathologic fractures of the processes and spontaneous expulsion of the sequestrae.", "contents": "Osteonecrosis of hyoid bone and thyroid cartilage. A 55-year-old man with carcinoma of the right pyriform fossa was treated with cobalt therapy. Subsequently, osteonecrosis of the right greater horn and the right superior horn of the thyroid cartilage developed, followed by pathologic fractures of the processes and spontaneous expulsion of the sequestrae."}
{"id": "PMID:475655", "title": "Reversible compression neuropathy of the eighth cranial nerve from a large jugular foramen schwannoma.", "content": "Jugular foramen schwannomas are unusual tumors occasionally resulting in neural hearing losses. Two previously reported cases document preservation of hearing following removal of a jugular foramen schwannoma. I report the third such case, in which there was progressive auditory and vestibular dysfunction seen on serial preoperative auditory tests and recovery of function seen on postoperative auditory and vestibular tests. The presumed pathophysiologic feature sub-serving this observation is compression neuropathy. I discuss experiences with compression neuropathy in animals and man under other circumstances.", "contents": "Reversible compression neuropathy of the eighth cranial nerve from a large jugular foramen schwannoma. Jugular foramen schwannomas are unusual tumors occasionally resulting in neural hearing losses. Two previously reported cases document preservation of hearing following removal of a jugular foramen schwannoma. I report the third such case, in which there was progressive auditory and vestibular dysfunction seen on serial preoperative auditory tests and recovery of function seen on postoperative auditory and vestibular tests. The presumed pathophysiologic feature sub-serving this observation is compression neuropathy. I discuss experiences with compression neuropathy in animals and man under other circumstances."}
{"id": "PMID:475657", "title": "An electron microscopic study on the lining mucosa of developing rat vocal cords.", "content": "To explain the developmental process of epithelial cells of true vocal cords, larynges obtained from 42 fetal and 36 developing young rats were examined using scanning and transmission electron microscopies. Although the membranous portion was indistinguishable from the arytenoid portion in the prenatal stage, there was no squamous cell epithelium on the glottis. The lining epithelium of vocal cords in 15, 16, and 17 day fetal rats was composed of cuboidal and columnar cells which had a primary cilium. Immediately after birth the membranous portion of vocal cords became recognizable, and ciliated cells present on vocal cords retrogressed rapidly while nonciliated squamous cells grew and extended. Results of the present study show that epithelial cells of ciliated columnar type covering vocal cords change remarkably to nonciliated squamous cells between prenatal and postnatal stages.", "contents": "An electron microscopic study on the lining mucosa of developing rat vocal cords. To explain the developmental process of epithelial cells of true vocal cords, larynges obtained from 42 fetal and 36 developing young rats were examined using scanning and transmission electron microscopies. Although the membranous portion was indistinguishable from the arytenoid portion in the prenatal stage, there was no squamous cell epithelium on the glottis. The lining epithelium of vocal cords in 15, 16, and 17 day fetal rats was composed of cuboidal and columnar cells which had a primary cilium. Immediately after birth the membranous portion of vocal cords became recognizable, and ciliated cells present on vocal cords retrogressed rapidly while nonciliated squamous cells grew and extended. Results of the present study show that epithelial cells of ciliated columnar type covering vocal cords change remarkably to nonciliated squamous cells between prenatal and postnatal stages."}
{"id": "PMID:475658", "title": "Nerve supply to the inner sensory cells in a human cochlea.", "content": "A choclear apical turn taken from a 66-year-old woman showed an area with marked decrease in number of the inner sensory cells. After observation by scanning electron microscope, the same specimen was thin-sectioned tangentially to the osseous spiral lamina. Numbers of myelinated nerve fibers were counted and the thickness of the fiber was measured by computed measuring equipment. Compared to the area with inner sensory cells intact, the area without inner sensory cells showed a 70% decrease in number of myelinated fibers. These degenerated fibers might have had intimate relationship with the inner sensory cells, probably being the afferent nerve supply to the inner sensory cells.", "contents": "Nerve supply to the inner sensory cells in a human cochlea. A choclear apical turn taken from a 66-year-old woman showed an area with marked decrease in number of the inner sensory cells. After observation by scanning electron microscope, the same specimen was thin-sectioned tangentially to the osseous spiral lamina. Numbers of myelinated nerve fibers were counted and the thickness of the fiber was measured by computed measuring equipment. Compared to the area with inner sensory cells intact, the area without inner sensory cells showed a 70% decrease in number of myelinated fibers. These degenerated fibers might have had intimate relationship with the inner sensory cells, probably being the afferent nerve supply to the inner sensory cells."}
{"id": "PMID:475659", "title": "[On the interpretation of B\u00e9k\u00e9sy audiograms (author's transl)].", "content": "A group of 112 subjects with sensorineural hearing loss was checked by an audiological test battery consisting of: pure tone audiometry, SISI Test, tone decay test and fixed frequency B\u00e9k\u00e9sy audiometry. Classification of B\u00e9k\u00e9sy tracings by two audiologists showes good agreement when introducing a hybrid type I/II, which completes Jerger's classification system of types I--IV. The results of our investigation exhibit no significant differences of manual pure tone threshold and automatic tracing contrary to some other authors. Finally the correlation of diagnosis and type of B\u00e9k\u00e9sy audiogram is discussed.", "contents": "[On the interpretation of B\u00e9k\u00e9sy audiograms (author's transl)]. A group of 112 subjects with sensorineural hearing loss was checked by an audiological test battery consisting of: pure tone audiometry, SISI Test, tone decay test and fixed frequency B\u00e9k\u00e9sy audiometry. Classification of B\u00e9k\u00e9sy tracings by two audiologists showes good agreement when introducing a hybrid type I/II, which completes Jerger's classification system of types I--IV. The results of our investigation exhibit no significant differences of manual pure tone threshold and automatic tracing contrary to some other authors. Finally the correlation of diagnosis and type of B\u00e9k\u00e9sy audiogram is discussed."}
{"id": "PMID:475660", "title": "DC potential and K+ activity in experimental endolymphatic hydrops.", "content": "No alteration in K+ activity of endolymph and perilymph was seen during development of endolymphatic hydrops in guinea pigs. The DC-potential was not changed during enlargement of endolymphatic space.", "contents": "DC potential and K+ activity in experimental endolymphatic hydrops. No alteration in K+ activity of endolymph and perilymph was seen during development of endolymphatic hydrops in guinea pigs. The DC-potential was not changed during enlargement of endolymphatic space."}
{"id": "PMID:475661", "title": "Early auditory-evoked responses in the cat: rate effects.", "content": "The brain-stem electric responses (BSER) of the cat have been evoked with a 4-kHz tone burst at three stimulus intensity levels, and at interstimulus intervals (ISI) varying fromm 500 to 20 ms. Response amplitude and latency have also been compared in different post-natal age groups, and in bilaterally hearing and unilaterally deaf animals. Increasing reptition rates produce greater latency changes in the youngest animals, whilst all age groups show amplitude decrement at shorter ISI, apart from wave III which increases in amplitude, particularly in bilaterally hearing cats at higher stimulus intensity levels. Wave I shows greater amplitude reduction than do the succeeding waves, which may indicate that the primary cochlear nuclei contribute to later waves of the BSER.", "contents": "Early auditory-evoked responses in the cat: rate effects. The brain-stem electric responses (BSER) of the cat have been evoked with a 4-kHz tone burst at three stimulus intensity levels, and at interstimulus intervals (ISI) varying fromm 500 to 20 ms. Response amplitude and latency have also been compared in different post-natal age groups, and in bilaterally hearing and unilaterally deaf animals. Increasing reptition rates produce greater latency changes in the youngest animals, whilst all age groups show amplitude decrement at shorter ISI, apart from wave III which increases in amplitude, particularly in bilaterally hearing cats at higher stimulus intensity levels. Wave I shows greater amplitude reduction than do the succeeding waves, which may indicate that the primary cochlear nuclei contribute to later waves of the BSER."}
{"id": "PMID:475662", "title": "Two-tone masking and auditory critical bandwidths.", "content": "Two-tone masking of 1,000-Hz signals was investigated in 5 normally-hearing listerners. The results indicate a consistent increase in critical bandwidth with level of the two masking tones (approximately 3%/dB). Irregularities in the individual two-tone masking functions led to further investigations with a single listener. For subcritical frequency separations between masking tones, -10 dB/decade masking slopes were demonstrated along with large dips or notches in the two-tone masking functions. Both the -10 dB/decade masking slopes and the large notches were replicated in detail when only the lower-frequency tones of the two-tone masking pairs were employed as maskers. Separate masking bands were then introduced that eliminated the -10 dB/decade masking slope and the large notch. It is concluded that the two-tone masking experiment is really a single-tone experiment, and that the detection of beats, roughness, and combination tones determines the form of single-tone and two-tone masking functions. If critical bnadwidth estimates are to be obtained from these experiments, it is most appropriate to refer to those estimates as \"critical bandwidths for tonal interaction\".", "contents": "Two-tone masking and auditory critical bandwidths. Two-tone masking of 1,000-Hz signals was investigated in 5 normally-hearing listerners. The results indicate a consistent increase in critical bandwidth with level of the two masking tones (approximately 3%/dB). Irregularities in the individual two-tone masking functions led to further investigations with a single listener. For subcritical frequency separations between masking tones, -10 dB/decade masking slopes were demonstrated along with large dips or notches in the two-tone masking functions. Both the -10 dB/decade masking slopes and the large notches were replicated in detail when only the lower-frequency tones of the two-tone masking pairs were employed as maskers. Separate masking bands were then introduced that eliminated the -10 dB/decade masking slope and the large notch. It is concluded that the two-tone masking experiment is really a single-tone experiment, and that the detection of beats, roughness, and combination tones determines the form of single-tone and two-tone masking functions. If critical bnadwidth estimates are to be obtained from these experiments, it is most appropriate to refer to those estimates as \"critical bandwidths for tonal interaction\"."}
{"id": "PMID:475663", "title": "Two experiments on subtle mid-frequency hearing loss and its influence on word discrimination in noise-exposed listeners.", "content": "The present study consisted of two experiments. Experiment I was directed toward the detection of subtle mid-frequency cochlear dysfunction and the determination of word discrimination in noise for persons with noise-induced hearing loss. The presence of significantly elevated aural-overload thresholds, consistent with the presence of cochlear pathology in regions of normal pure-tone sensitivity, confirmed previous findings obtained with this subject population by other investigators. In addition, a marked reduction in word discrimination was observed in the presence of a background of competing noise. For experiment II, normal hearers listened to consonant-nucleus-consonant monosyllables filtered to match the mean audiometric configuration of the noise-exposed subjects in experiment I. Results showed significantly better performance with these filtered words by the normally hearing subjects than that by the noise-exposed group for unfiltered materials. The results of the second experiment were interpreted as providing further support for the presence of subtle auditory dysfunction in the mid-frequencies despite normal threshold sensitivity in this same frequency region.", "contents": "Two experiments on subtle mid-frequency hearing loss and its influence on word discrimination in noise-exposed listeners. The present study consisted of two experiments. Experiment I was directed toward the detection of subtle mid-frequency cochlear dysfunction and the determination of word discrimination in noise for persons with noise-induced hearing loss. The presence of significantly elevated aural-overload thresholds, consistent with the presence of cochlear pathology in regions of normal pure-tone sensitivity, confirmed previous findings obtained with this subject population by other investigators. In addition, a marked reduction in word discrimination was observed in the presence of a background of competing noise. For experiment II, normal hearers listened to consonant-nucleus-consonant monosyllables filtered to match the mean audiometric configuration of the noise-exposed subjects in experiment I. Results showed significantly better performance with these filtered words by the normally hearing subjects than that by the noise-exposed group for unfiltered materials. The results of the second experiment were interpreted as providing further support for the presence of subtle auditory dysfunction in the mid-frequencies despite normal threshold sensitivity in this same frequency region."}
{"id": "PMID:475664", "title": "Age effect in hearing - a comparative analysis of published threshold data.", "content": "Numerical data from the literature on presbyacusis, in the form of pure-tone hearing threshold levels as a function of age, are subjected to critical evaluation and analysis. A formula is derived for predicting the age effect for otologically screened groups of males and females. Further, the complete distribution of hearing levels for each audiometric frequency in the range 0.125-12 kHz and at any age can be estimated with the aid of tabulated coefficients and some simple rules. The study was undertaken in connection with the work of ISO on standardization of normative audiometric data.", "contents": "Age effect in hearing - a comparative analysis of published threshold data. Numerical data from the literature on presbyacusis, in the form of pure-tone hearing threshold levels as a function of age, are subjected to critical evaluation and analysis. A formula is derived for predicting the age effect for otologically screened groups of males and females. Further, the complete distribution of hearing levels for each audiometric frequency in the range 0.125-12 kHz and at any age can be estimated with the aid of tabulated coefficients and some simple rules. The study was undertaken in connection with the work of ISO on standardization of normative audiometric data."}
{"id": "PMID:475665", "title": "Physical and subjective correlates of earmould occlusion.", "content": "Physical and subjective aspects of earmould occlusion are examined and related. In particular, measurements made in an ear-like coupler for various venting arrangements are compared with those obtained subjectively by a loudness balance procedure. The results demonstrate the effectiveness of an open (completely non-occluding) earmould at low-frequency sound reduction but indicate some of the factors which make its individual effect difficult to predict. It is further seen the group of hearing-impaired persons was less able to make systematic judgements on sound quality than was the control group of normally hearing persons.", "contents": "Physical and subjective correlates of earmould occlusion. Physical and subjective aspects of earmould occlusion are examined and related. In particular, measurements made in an ear-like coupler for various venting arrangements are compared with those obtained subjectively by a loudness balance procedure. The results demonstrate the effectiveness of an open (completely non-occluding) earmould at low-frequency sound reduction but indicate some of the factors which make its individual effect difficult to predict. It is further seen the group of hearing-impaired persons was less able to make systematic judgements on sound quality than was the control group of normally hearing persons."}
{"id": "PMID:475666", "title": "Enhancement of nitroblue tetrazolium dye reduction by leucocytes exposed to a factor released from sensitised lymphocytes.", "content": "Leucocytes from purified protein derivative (PPD) skin test positive individuals, after exposure to PPD in vitro, show a significant increase in nitroblue tetrazolium (NBT) dye reduction when compared to leucocytes from PPD negative donors. The active principle was shown to be soluble, and was released from lymphocytes after a rapid, immunologically specific interaction with the sensitising antigen.", "contents": "Enhancement of nitroblue tetrazolium dye reduction by leucocytes exposed to a factor released from sensitised lymphocytes. Leucocytes from purified protein derivative (PPD) skin test positive individuals, after exposure to PPD in vitro, show a significant increase in nitroblue tetrazolium (NBT) dye reduction when compared to leucocytes from PPD negative donors. The active principle was shown to be soluble, and was released from lymphocytes after a rapid, immunologically specific interaction with the sensitising antigen."}
{"id": "PMID:475667", "title": "Production of tremorgenic toxins by Penicillium janthinellum Biourge: a possible aetiological factor in ryegrass staggers.", "content": "Topsoil, herbage and faeces collected during an outbreak of ryegrass staggers in sheep were examined for tremorgenic penicillia. No such fungi were recovered from the plant material, but they were found among the predominant fungi in the soil and faecal samples. The commonest species of Penicillium, and almost the only tremorgenic species encountered, was Penicillium janthinellum Biourge. When fed to sheep, the mycelium of this fungus evoked a number of the clinical signs seen in field cases of ryegrass staggers. Two tremorgenic toxins were isolated from the mycelial felts and available evidence indicates that they are verruculogen and fumitremorgin A. P. janthinellum also produced these tremorgens when cultured in moist, autoclaved soil, but not in unheated soil. The results obtained from this study are in accord with the hypothesis that ryegrass staggers is caused by tremorgenic mycotoxins.", "contents": "Production of tremorgenic toxins by Penicillium janthinellum Biourge: a possible aetiological factor in ryegrass staggers. Topsoil, herbage and faeces collected during an outbreak of ryegrass staggers in sheep were examined for tremorgenic penicillia. No such fungi were recovered from the plant material, but they were found among the predominant fungi in the soil and faecal samples. The commonest species of Penicillium, and almost the only tremorgenic species encountered, was Penicillium janthinellum Biourge. When fed to sheep, the mycelium of this fungus evoked a number of the clinical signs seen in field cases of ryegrass staggers. Two tremorgenic toxins were isolated from the mycelial felts and available evidence indicates that they are verruculogen and fumitremorgin A. P. janthinellum also produced these tremorgens when cultured in moist, autoclaved soil, but not in unheated soil. The results obtained from this study are in accord with the hypothesis that ryegrass staggers is caused by tremorgenic mycotoxins."}
{"id": "PMID:475668", "title": "A method for the analysis of sigmoid stimulus-response curves.", "content": "This paper describes a method, using an interactive computer programme, for fitting a curve to data which is expected to assume a sigmoid form. The programme is specifically designed to generate best estimates of the limits and maximum slope of stimulus-response curves for the carotid sinus baroreceptor reflex, but is applicable to curve-fitting tasks in other fields such as immunology, pharmacology, biochemistry and neurophysiology.", "contents": "A method for the analysis of sigmoid stimulus-response curves. This paper describes a method, using an interactive computer programme, for fitting a curve to data which is expected to assume a sigmoid form. The programme is specifically designed to generate best estimates of the limits and maximum slope of stimulus-response curves for the carotid sinus baroreceptor reflex, but is applicable to curve-fitting tasks in other fields such as immunology, pharmacology, biochemistry and neurophysiology."}
{"id": "PMID:475669", "title": "The binding of urate by plasma proteins.", "content": "Protein binding of urate may have some pertinence to the pathogenesis of gout. However, binding studies have been hampered by problems with in vitro methodology and by the problem of relating the results of in vitro studies to the physiological situation. In the present study urate binding was determined by an ultrafiltration procedure. All manipulations were performed anaerobically and at 37 degrees in order to maintain the sample under physiological conditions. Normal urate bound was 15 +/- 7.5% in males and 10 +/- 6.6% in females. Urate binding did not correlate significantly with either the albumin or total urate concentration. It is suggested that the interaction between protein and urate is influenced by a number of factors other than the concentrations of free urate and binding protein. Some possible ones are discussed. The techniques described might usefully be applied to a study of urate binding in various pathological states.", "contents": "The binding of urate by plasma proteins. Protein binding of urate may have some pertinence to the pathogenesis of gout. However, binding studies have been hampered by problems with in vitro methodology and by the problem of relating the results of in vitro studies to the physiological situation. In the present study urate binding was determined by an ultrafiltration procedure. All manipulations were performed anaerobically and at 37 degrees in order to maintain the sample under physiological conditions. Normal urate bound was 15 +/- 7.5% in males and 10 +/- 6.6% in females. Urate binding did not correlate significantly with either the albumin or total urate concentration. It is suggested that the interaction between protein and urate is influenced by a number of factors other than the concentrations of free urate and binding protein. Some possible ones are discussed. The techniques described might usefully be applied to a study of urate binding in various pathological states."}
{"id": "PMID:475670", "title": "A comparison of methods for measuring intestinal antibodies against Vibrio cholerae in mice.", "content": "A re-evaluation was made of the efficiency with which some of the commonly used assays would detect intestinal antibodies. The data indicate that the most sensitive assays for the detection of intestinal antibodies are the baby mouse protection test and the radioimmunoassay. The reasons for the lack of sensitivity with other assay methods are discussed.", "contents": "A comparison of methods for measuring intestinal antibodies against Vibrio cholerae in mice. A re-evaluation was made of the efficiency with which some of the commonly used assays would detect intestinal antibodies. The data indicate that the most sensitive assays for the detection of intestinal antibodies are the baby mouse protection test and the radioimmunoassay. The reasons for the lack of sensitivity with other assay methods are discussed."}
{"id": "PMID:475671", "title": "Intestinal antibody to Vibrio cholerae in immunised mice.", "content": "The immune response of the mouse to priming and booster doses of V. cholerae was studied to establish whether serum antibody could be used as a correlate of local immunity. Serum antibody titres following oral boosting of orally-primed animals were shown to reflect the state of local intestinal immunity. This was not the case when the same oral booster dose was given to parenterally-primed animals. These results were discussed in relation to the human endemic situation. The highest titres of intestinal protective antibodies were found following combination of the oral and parenteral routes of immunisation. Various killed or extracted preparations of V. cholerae were used as oral vaccines to test their ability to induce protective antibodies in the gut. Only Boivin antigen was capable of inducing as good an intestinal antibody response as would the living organism.", "contents": "Intestinal antibody to Vibrio cholerae in immunised mice. The immune response of the mouse to priming and booster doses of V. cholerae was studied to establish whether serum antibody could be used as a correlate of local immunity. Serum antibody titres following oral boosting of orally-primed animals were shown to reflect the state of local intestinal immunity. This was not the case when the same oral booster dose was given to parenterally-primed animals. These results were discussed in relation to the human endemic situation. The highest titres of intestinal protective antibodies were found following combination of the oral and parenteral routes of immunisation. Various killed or extracted preparations of V. cholerae were used as oral vaccines to test their ability to induce protective antibodies in the gut. Only Boivin antigen was capable of inducing as good an intestinal antibody response as would the living organism."}
{"id": "PMID:475672", "title": "Lymphocyte sensitization in sheep inoculated with extracts of spontaneously occurring malignant lymphomas.", "content": "A macrophage migration inhibition test showed that the blood lymphocytes of 15 of 27 sheep inoculated in utero or at birth with disrupted cells from ovine malignant lymphomas responded in culture to antigens derived from three other lymphomas. A close association was seen between animals whose lymphocytes were antigen-sensitive and those which developed lymphocytosis. This association and the specificity of reaction between the various tumour antigens employed in the tests is interpreted as further evidence toward a viral aetiology in ovine malignant lymphoma.", "contents": "Lymphocyte sensitization in sheep inoculated with extracts of spontaneously occurring malignant lymphomas. A macrophage migration inhibition test showed that the blood lymphocytes of 15 of 27 sheep inoculated in utero or at birth with disrupted cells from ovine malignant lymphomas responded in culture to antigens derived from three other lymphomas. A close association was seen between animals whose lymphocytes were antigen-sensitive and those which developed lymphocytosis. This association and the specificity of reaction between the various tumour antigens employed in the tests is interpreted as further evidence toward a viral aetiology in ovine malignant lymphoma."}
{"id": "PMID:475673", "title": "Radioimmunoassay of secretin in serum.", "content": "A radioimmunoassay for the measurement of immunoreactive secretin has been developed. The assay is specific and sensitive so that 2.8 fmol/ml can be measured. Basal secretin levels in man are generally undetectable and a protein-rich meal does not elicit a secretin response. However, the intraduodenal instillation of HCl leads to a prompt rise in circulating immunoreactive secretin and exogenous secretin administered intravenously is easily and accurately measured.", "contents": "Radioimmunoassay of secretin in serum. A radioimmunoassay for the measurement of immunoreactive secretin has been developed. The assay is specific and sensitive so that 2.8 fmol/ml can be measured. Basal secretin levels in man are generally undetectable and a protein-rich meal does not elicit a secretin response. However, the intraduodenal instillation of HCl leads to a prompt rise in circulating immunoreactive secretin and exogenous secretin administered intravenously is easily and accurately measured."}
{"id": "PMID:475674", "title": "Presumptive 1/29 autosomal translocation in Australian cattle.", "content": "A cytogenetical study was made of 9 descendants of a Charolais bull, heterozygous for a presumptive 1/29 translocation, three of the dams of some of these descendants, and three unrelated bulls which were mated to some of the descendants. Photographic karyotypes of Giemsa stained and C-banded chromosomes were prepared for each animal. The dams of the progeny showed no chromosomal abnormalities indicating that translocations were inherited from the sire. Three daughters of the Charolais bull and two of his grand daughters were heterozygous for the translocation, with a modal chromosomal number of 59, while the remaining three daughters and their progeny possessed normal karyotypes. No phenotypic abnormalities were observed in the animals examined. Measurements of the arms of the chromosomes suggested that the translocation chromosome (a large submetacentric) contained chromosomes 1 and 29. The submetacentric translocation chromosome had a single C-band, the two submetacentric X chromosomes showed no C-bands and each acrocentric autosome had a single C-band. All cattle heterozygous for the translocation showed normal fertility.", "contents": "Presumptive 1/29 autosomal translocation in Australian cattle. A cytogenetical study was made of 9 descendants of a Charolais bull, heterozygous for a presumptive 1/29 translocation, three of the dams of some of these descendants, and three unrelated bulls which were mated to some of the descendants. Photographic karyotypes of Giemsa stained and C-banded chromosomes were prepared for each animal. The dams of the progeny showed no chromosomal abnormalities indicating that translocations were inherited from the sire. Three daughters of the Charolais bull and two of his grand daughters were heterozygous for the translocation, with a modal chromosomal number of 59, while the remaining three daughters and their progeny possessed normal karyotypes. No phenotypic abnormalities were observed in the animals examined. Measurements of the arms of the chromosomes suggested that the translocation chromosome (a large submetacentric) contained chromosomes 1 and 29. The submetacentric translocation chromosome had a single C-band, the two submetacentric X chromosomes showed no C-bands and each acrocentric autosome had a single C-band. All cattle heterozygous for the translocation showed normal fertility."}
{"id": "PMID:475675", "title": "Distribution of Cysticercus bovis in lightly infected young cattle.", "content": "Sixty-seven young cattle grazed on a sewage farm since birth were slaughtered and the carcases finely dissected in search of Cysticercus bovis. Forty-seven (70%) were infected, but only 49% of infected cattle had cysts in tissues considered to be sites of predilection and only 19.8% of all cysts found were located in these sites. Most of the remaining cysts were distributed randomly throughout the masculature.", "contents": "Distribution of Cysticercus bovis in lightly infected young cattle. Sixty-seven young cattle grazed on a sewage farm since birth were slaughtered and the carcases finely dissected in search of Cysticercus bovis. Forty-seven (70%) were infected, but only 49% of infected cattle had cysts in tissues considered to be sites of predilection and only 19.8% of all cysts found were located in these sites. Most of the remaining cysts were distributed randomly throughout the masculature."}
{"id": "PMID:475676", "title": "Diagnosis of Johne's disease in cattle using mesenteric lymph node biopsy: accuracy in clinical suspects.", "content": "Histopathological examination of biopsies of mesenteric lymph node obtained from the region of the terminal ileum proved to be a very accurate method of diagnosing Johne's disease in the living animal. Identical conclusions were reached in 100 clinical suspects following examination of either mesenteric lymph nodes alone, or a wider range of routine specimens including ileum, caecum, colon and ileocaecal lymph nodes. A method of obtaining biopsy specimens from mesenteric lymph nodes is described and is recommended when an early definitive diagnosis is required.", "contents": "Diagnosis of Johne's disease in cattle using mesenteric lymph node biopsy: accuracy in clinical suspects. Histopathological examination of biopsies of mesenteric lymph node obtained from the region of the terminal ileum proved to be a very accurate method of diagnosing Johne's disease in the living animal. Identical conclusions were reached in 100 clinical suspects following examination of either mesenteric lymph nodes alone, or a wider range of routine specimens including ileum, caecum, colon and ileocaecal lymph nodes. A method of obtaining biopsy specimens from mesenteric lymph nodes is described and is recommended when an early definitive diagnosis is required."}
{"id": "PMID:475677", "title": "Bromosulphophthalein clearance rates in sheep with pyrrolizidine liver damage.", "content": "Sheep fed a ration containing 50% of dried Heliotropium europaeum showed a marked decline in bromosulphophthalein (BSP) fractional clearance rate during the first 3 months feeding. Thereafter, the response of individual animals varied widely on a time basis, although 3 groups could be identified. In the terminal stages, mean clearance rates were below 20% of initial values, with some sheep showing a decline in excess of 90%. Loss of liver functional capacity was generally much greater than indicated by the degree of damage revealed by histopathology. Thus, a suitably modified test could have considerable prognostic value in the field. In this investigation, all sheep with clearance rates below 0.15 died when exposed to a further period of H. europaeum feeding.", "contents": "Bromosulphophthalein clearance rates in sheep with pyrrolizidine liver damage. Sheep fed a ration containing 50% of dried Heliotropium europaeum showed a marked decline in bromosulphophthalein (BSP) fractional clearance rate during the first 3 months feeding. Thereafter, the response of individual animals varied widely on a time basis, although 3 groups could be identified. In the terminal stages, mean clearance rates were below 20% of initial values, with some sheep showing a decline in excess of 90%. Loss of liver functional capacity was generally much greater than indicated by the degree of damage revealed by histopathology. Thus, a suitably modified test could have considerable prognostic value in the field. In this investigation, all sheep with clearance rates below 0.15 died when exposed to a further period of H. europaeum feeding."}
{"id": "PMID:475679", "title": "Resistance to thiabendazole in a field population of Ostertagia circumcincta from sheep.", "content": "A population of Ostertagia circumcincta from sheep has been found which, in field and laboratory trials, showed a lack of response to the recommended dose (44 mg/kg) of thiabendazole, suggesting that a degree of resistance exists to this anthelmintic. Preliminary experiments indicated a less than expected response to levamisole. This was not confirmed in a laboratory trial and therefore resistance to this anthelmintic has not been shown conclusively.", "contents": "Resistance to thiabendazole in a field population of Ostertagia circumcincta from sheep. A population of Ostertagia circumcincta from sheep has been found which, in field and laboratory trials, showed a lack of response to the recommended dose (44 mg/kg) of thiabendazole, suggesting that a degree of resistance exists to this anthelmintic. Preliminary experiments indicated a less than expected response to levamisole. This was not confirmed in a laboratory trial and therefore resistance to this anthelmintic has not been shown conclusively."}
{"id": "PMID:475680", "title": "Efficacy of oxfendazole against inhibited Ostertagia ostertagi in naturally infected cattle.", "content": "Oxfendazole was administered to pregnant cows at 2.5 and 5 mg/kg body weight to determine the anthelmintic efficacy against naturally acquired larvae which became inhibited at the early 4th stage. The experimental design included three groups of orally-treated cows, that is, 10 placebo treated control cows, 11 cows treated with 2.5 mg/kg of oxfendazole and 10 cows treated with 5.0 mg/kg of oxfendazole. Oxfendazole at 2.5 mg/kg body weight was 82 and 94% effective against EL-4 and adult O. ostertagi, respectively. At 5 mg/kg, Oxfendazole was 95 and 99% effective against EL-4 And adult O. ostertagi, respectively. The results suggested the use of a field dosage level of 5 mg/kg body weight oxfendazole where inhibited larvae may be encountered.", "contents": "Efficacy of oxfendazole against inhibited Ostertagia ostertagi in naturally infected cattle. Oxfendazole was administered to pregnant cows at 2.5 and 5 mg/kg body weight to determine the anthelmintic efficacy against naturally acquired larvae which became inhibited at the early 4th stage. The experimental design included three groups of orally-treated cows, that is, 10 placebo treated control cows, 11 cows treated with 2.5 mg/kg of oxfendazole and 10 cows treated with 5.0 mg/kg of oxfendazole. Oxfendazole at 2.5 mg/kg body weight was 82 and 94% effective against EL-4 and adult O. ostertagi, respectively. At 5 mg/kg, Oxfendazole was 95 and 99% effective against EL-4 And adult O. ostertagi, respectively. The results suggested the use of a field dosage level of 5 mg/kg body weight oxfendazole where inhibited larvae may be encountered."}
{"id": "PMID:475681", "title": "Effect of adding superphosphate to the drinking water on the fertility of dairy cows.", "content": "Mean serum inorganic phosphorus concentrations of cows in an infertile dairy herd of 170 cows in the Hunter Valley increased from less than 4.5 mg/100 ml to greater than 5.8 mg/100 ml after defluorinated superphosphate was added to their drinking water at the rate of 2.5 kg/450 1 once weekly from September, 1976. The first service pregnancy rate increased from 36.5% to 63.2%, the mean calving to conception interval decreased from 109 days to 85 days and the number of cows culled each year for infertility fell from 15 to 5. The pasture fed to the milking cows contained on average greater than 17% crude protein, greater than or equal to 0.39% phosphorus, greater than or equal to 9 ppm copper and 38--46 ppm manganese according to the dominant species. The Ca/P ratio ranged from 2 to 5.5:1. Of these, only the Ca/P ratio and the manganese concentrations appeared to be abnormal.", "contents": "Effect of adding superphosphate to the drinking water on the fertility of dairy cows. Mean serum inorganic phosphorus concentrations of cows in an infertile dairy herd of 170 cows in the Hunter Valley increased from less than 4.5 mg/100 ml to greater than 5.8 mg/100 ml after defluorinated superphosphate was added to their drinking water at the rate of 2.5 kg/450 1 once weekly from September, 1976. The first service pregnancy rate increased from 36.5% to 63.2%, the mean calving to conception interval decreased from 109 days to 85 days and the number of cows culled each year for infertility fell from 15 to 5. The pasture fed to the milking cows contained on average greater than 17% crude protein, greater than or equal to 0.39% phosphorus, greater than or equal to 9 ppm copper and 38--46 ppm manganese according to the dominant species. The Ca/P ratio ranged from 2 to 5.5:1. Of these, only the Ca/P ratio and the manganese concentrations appeared to be abnormal."}
{"id": "PMID:475682", "title": "Activity against Ostertagia ostertagi of low doses of oxfendazole continuously released from intraruminal capsules in cattle.", "content": "Slow release from intraruminal capsules of 0.48 mg/kg oxfendazole per day for 5.5 days gave percentage efficiencies of 99 +/- 0.6, 86.6 +/- 6.3 and 93.1 +/- 4.5 for the removal of adult worms, developing 4th stages and inhibited larvae of Ostertagia ostertagi respectively. These efficiencies were not significantly different from those obtained from a single oral dose of 2.5 mg/kg. Peak plasma levels of oxfendazole were similar for the 2 types of administration but high levels were maintained longer in cattle given capsules.", "contents": "Activity against Ostertagia ostertagi of low doses of oxfendazole continuously released from intraruminal capsules in cattle. Slow release from intraruminal capsules of 0.48 mg/kg oxfendazole per day for 5.5 days gave percentage efficiencies of 99 +/- 0.6, 86.6 +/- 6.3 and 93.1 +/- 4.5 for the removal of adult worms, developing 4th stages and inhibited larvae of Ostertagia ostertagi respectively. These efficiencies were not significantly different from those obtained from a single oral dose of 2.5 mg/kg. Peak plasma levels of oxfendazole were similar for the 2 types of administration but high levels were maintained longer in cattle given capsules."}
{"id": "PMID:475701", "title": "Aircrew helmet protection against potential cerebral concussion in low-magnitude impacts.", "content": "The response of the Gentex DH-151 (contact type) and Gentex 411 (suspension type) aircrew helmets to low-magnitude impacts, such as those sometimes encountered during cockpit buffeting, in ejection, and in parachute landings, was studied to augment the data base on helmet performance. The helmets, mounted on a Hodgson headform, were dropped on the crown and rear at impact velocities up to 4.97 m/s. Acceleration time histories were tape recorded and digitized and Gadd Severity Indices (GSI), among others, were calculated from the resultant acceleration curve. Both helmets kept the GSI below predicted concussion thresholds at 4.97 m/s and were considered to perform well on initial impacts. On second impacts, the GSI rose considerably because the shell and liner of the DH-151 cracked and the suspension of the \"141\" stretched during the first blow. Improvement of the multiple impact performance of both helmets appears desirable, although the suspension helmet performed slightly better than the contact helmet with respect to the criterion used.", "contents": "Aircrew helmet protection against potential cerebral concussion in low-magnitude impacts. The response of the Gentex DH-151 (contact type) and Gentex 411 (suspension type) aircrew helmets to low-magnitude impacts, such as those sometimes encountered during cockpit buffeting, in ejection, and in parachute landings, was studied to augment the data base on helmet performance. The helmets, mounted on a Hodgson headform, were dropped on the crown and rear at impact velocities up to 4.97 m/s. Acceleration time histories were tape recorded and digitized and Gadd Severity Indices (GSI), among others, were calculated from the resultant acceleration curve. Both helmets kept the GSI below predicted concussion thresholds at 4.97 m/s and were considered to perform well on initial impacts. On second impacts, the GSI rose considerably because the shell and liner of the DH-151 cracked and the suspension of the \"141\" stretched during the first blow. Improvement of the multiple impact performance of both helmets appears desirable, although the suspension helmet performed slightly better than the contact helmet with respect to the criterion used."}
{"id": "PMID:475702", "title": "Physiological effects of a military training program on male and female cadets.", "content": "Tested at the beginning and the end of the 6-week training program which all incoming cadets (plebes) undergo upon entering the U.S. Military Academy were 29 males and 26 females (17-21 years old). The aerobic training consisted of running for 30 min 5-6 d/week at varied speeds depending upon performance in an initial 1.5-mile run test. Females responded to training with a significant increase (p is less than 0.001) in VO2 max from 46.0 +/- 1.0 to 49.7 +/- 0.8 ml/kg.min (7.9%). Males did not increase their initial VO2 max (59.4 +/- 1.1 ml/kg.min) significantly. Both groups significantly reduced HRmax and percent body fat. Their initial VO2 max values and activity history accounted for the lack of a significant increase in this highly-fit population of males. Blood lactates were significantly decreased (p is less than 0.05) at the same two submaximal workloads after training. The initial difference in aerobic power between males and females was reduced from 22% to 18%.", "contents": "Physiological effects of a military training program on male and female cadets. Tested at the beginning and the end of the 6-week training program which all incoming cadets (plebes) undergo upon entering the U.S. Military Academy were 29 males and 26 females (17-21 years old). The aerobic training consisted of running for 30 min 5-6 d/week at varied speeds depending upon performance in an initial 1.5-mile run test. Females responded to training with a significant increase (p is less than 0.001) in VO2 max from 46.0 +/- 1.0 to 49.7 +/- 0.8 ml/kg.min (7.9%). Males did not increase their initial VO2 max (59.4 +/- 1.1 ml/kg.min) significantly. Both groups significantly reduced HRmax and percent body fat. Their initial VO2 max values and activity history accounted for the lack of a significant increase in this highly-fit population of males. Blood lactates were significantly decreased (p is less than 0.05) at the same two submaximal workloads after training. The initial difference in aerobic power between males and females was reduced from 22% to 18%."}
{"id": "PMID:475703", "title": "Doppler detection of thresholds for decompression-induced venous gas emboli in the awake rat.", "content": "Awake, unrestrained rats with chronic implants of a Doppler probe on the vena cava, were saturated in a small plexiglass chamber at a maximum pressure (P1), rapidly decompressed to a predetermined pressure (P2) and then monitored for the presence of intravascular bubbles. If there were no indication of bubbles within 1 h, the decompression was considered bubble free. For each saturation pressure, an increasing pressure difference (deltaP = P1 - P2) was tried on each exposure until the threshold for bubble detection was found. The relationship deltaP = a P1 + b, where a and b are constants defining the threshold for bubble detection, was experimentally determined at pressures from 3-10 ATA. The depth-dependent linear relationship deltaP = 0.38P, + 1.29 (r = 0.97, P is less than 0.001) was found for first exposures and deltaP = 0.62 P1 + 0.16 (r = 0.99, p is less than 0.001) for repeat exposures. The chronic Doppler preparation allows a more sensitive threshold determination than past methods for small animals and also allows repeat pressure exposures without the effects of a severe decompression stress.", "contents": "Doppler detection of thresholds for decompression-induced venous gas emboli in the awake rat. Awake, unrestrained rats with chronic implants of a Doppler probe on the vena cava, were saturated in a small plexiglass chamber at a maximum pressure (P1), rapidly decompressed to a predetermined pressure (P2) and then monitored for the presence of intravascular bubbles. If there were no indication of bubbles within 1 h, the decompression was considered bubble free. For each saturation pressure, an increasing pressure difference (deltaP = P1 - P2) was tried on each exposure until the threshold for bubble detection was found. The relationship deltaP = a P1 + b, where a and b are constants defining the threshold for bubble detection, was experimentally determined at pressures from 3-10 ATA. The depth-dependent linear relationship deltaP = 0.38P, + 1.29 (r = 0.97, P is less than 0.001) was found for first exposures and deltaP = 0.62 P1 + 0.16 (r = 0.99, p is less than 0.001) for repeat exposures. The chronic Doppler preparation allows a more sensitive threshold determination than past methods for small animals and also allows repeat pressure exposures without the effects of a severe decompression stress."}
{"id": "PMID:475704", "title": "Psychiatric aspects of adaptation to long submarine missions.", "content": "The case histories of 261 submarines disqualified psychiatrically for further submarine duty revealed that the major symptomatology was emotional in nature, with character pathology second in frequency. The latter pathology resulted in disqualification much earlier than the former, a fact indicative of effective neurotic defenses. The submariners who show these slow-developing neurotic symptoms appear to be reacting maladaptively to the stresses of the 60-d submarine patrols. Some etiological factors appear to be related to the role of man occupies within the crew, to the nature of the mission itself and, perhaps, to the length of the submarine patrols.", "contents": "Psychiatric aspects of adaptation to long submarine missions. The case histories of 261 submarines disqualified psychiatrically for further submarine duty revealed that the major symptomatology was emotional in nature, with character pathology second in frequency. The latter pathology resulted in disqualification much earlier than the former, a fact indicative of effective neurotic defenses. The submariners who show these slow-developing neurotic symptoms appear to be reacting maladaptively to the stresses of the 60-d submarine patrols. Some etiological factors appear to be related to the role of man occupies within the crew, to the nature of the mission itself and, perhaps, to the length of the submarine patrols."}
{"id": "PMID:475705", "title": "Effect of diet on dichlorovinyl dimethyl phosphate toxicity in rats.", "content": "Young male albino rats (120+/-5 g) were kept for 30 d on the following synthetic diets--High Protein Diet (HPD): 59% casein; Low Protein Diet (LPD): 5% casein; High Fat Diet (HFD): 51% fat; and Standard Diet (SD); 19% casein, 11% fat, and 60% sucrose. Composition of diet per se did not significantly affect plasma and RBC cholinesterase (ChE) activity, Mortality of animals on different diets, due to administration of DDVP (Vapona, dichlorovos) 50 mg/kg i.p. was: SD: 37%; HPD: 26.5%, LPD: 53%; and HFD: 44%. Plasma ChE inhibition after 24 h of DDVP administration (in surviving animals) was SD: 42%; HPD: 52%; LPD: 55%; and HFD: 47%. Erythrocyte ChE inhibition was 40% in SD, HPD, and HFD and 60% in the LPD rats. Five days following DDVP administration, plasma ChE was normal and erythrocyte ChE was still significantly increased and erythrocyte ChE was normal.", "contents": "Effect of diet on dichlorovinyl dimethyl phosphate toxicity in rats. Young male albino rats (120+/-5 g) were kept for 30 d on the following synthetic diets--High Protein Diet (HPD): 59% casein; Low Protein Diet (LPD): 5% casein; High Fat Diet (HFD): 51% fat; and Standard Diet (SD); 19% casein, 11% fat, and 60% sucrose. Composition of diet per se did not significantly affect plasma and RBC cholinesterase (ChE) activity, Mortality of animals on different diets, due to administration of DDVP (Vapona, dichlorovos) 50 mg/kg i.p. was: SD: 37%; HPD: 26.5%, LPD: 53%; and HFD: 44%. Plasma ChE inhibition after 24 h of DDVP administration (in surviving animals) was SD: 42%; HPD: 52%; LPD: 55%; and HFD: 47%. Erythrocyte ChE inhibition was 40% in SD, HPD, and HFD and 60% in the LPD rats. Five days following DDVP administration, plasma ChE was normal and erythrocyte ChE was still significantly increased and erythrocyte ChE was normal."}
{"id": "PMID:475706", "title": "Effect of induced cyclic changes of deep body temperature on task performances.", "content": "Performance of three tests was studied during induced cycles of deep body temperature between limits of 37.8 degrees C and 38.9 degrees C. During heating phases skin temperature was 38.8 degrees C and during cooling it was 36.1 degrees C. A verbal transformation test, performed at the midpoint of each temperature cycle, showed no significant effect from the large differences in skin temperature and subjective comfort between heating and cooling. The test was considered to be insufficiently difficult. A pursuit rotor test and a colour/word interference test, performed at the end of the heating and cooling phases, showed mean decrements in performance of 15% and 4%, respectively. These results are related to the measured levels of deep body and skin temperature and to subjective assessments of comfort.", "contents": "Effect of induced cyclic changes of deep body temperature on task performances. Performance of three tests was studied during induced cycles of deep body temperature between limits of 37.8 degrees C and 38.9 degrees C. During heating phases skin temperature was 38.8 degrees C and during cooling it was 36.1 degrees C. A verbal transformation test, performed at the midpoint of each temperature cycle, showed no significant effect from the large differences in skin temperature and subjective comfort between heating and cooling. The test was considered to be insufficiently difficult. A pursuit rotor test and a colour/word interference test, performed at the end of the heating and cooling phases, showed mean decrements in performance of 15% and 4%, respectively. These results are related to the measured levels of deep body and skin temperature and to subjective assessments of comfort."}
{"id": "PMID:475707", "title": "Regional heat loss in resting man during immersion in 25.2 degrees C water.", "content": "Five male subjects having a wide range of relative body fat, 9.2-20.2%, were studied during total body immersion in water at 25.2 degrees C. The regional surface area of each subject was calculated from anthropometric data utilizing a segmental geometric model. Skin temperatures (Tsk) and regional skin heat loss were measured prior to and during 30 min immersion at 13 sites. During immersion, mean Tsk was 25.9 degrees C and remained significantly higher than the water temperature. A measurable temperature gradient for heat flow was observed from all body segments. Segimental temperature in water ranged from 26.7-25.4 degrees C, being warmest at the neck and coolest at the foot. Heat the flow per regional area was highest in the neck, 187 W/m2, and least at the foot, 12 W/m2. Heat flow from each body region was dependent on regional Tsk. Skinfold thickness was a minor factor in altering regional heat flow in the foot, hand, lower arm, upper arm, thigh, and calf; in the torso, neck, and head regions it was of major importance in detering heat loss.", "contents": "Regional heat loss in resting man during immersion in 25.2 degrees C water. Five male subjects having a wide range of relative body fat, 9.2-20.2%, were studied during total body immersion in water at 25.2 degrees C. The regional surface area of each subject was calculated from anthropometric data utilizing a segmental geometric model. Skin temperatures (Tsk) and regional skin heat loss were measured prior to and during 30 min immersion at 13 sites. During immersion, mean Tsk was 25.9 degrees C and remained significantly higher than the water temperature. A measurable temperature gradient for heat flow was observed from all body segments. Segimental temperature in water ranged from 26.7-25.4 degrees C, being warmest at the neck and coolest at the foot. Heat the flow per regional area was highest in the neck, 187 W/m2, and least at the foot, 12 W/m2. Heat flow from each body region was dependent on regional Tsk. Skinfold thickness was a minor factor in altering regional heat flow in the foot, hand, lower arm, upper arm, thigh, and calf; in the torso, neck, and head regions it was of major importance in detering heat loss."}
{"id": "PMID:475708", "title": "Human tilt perception in a dynamic environment.", "content": "This study examines the perception of tilt and linear acceleration as sensed through the utricular otolith of the vestibular system. Any sensation derived from the otolith is a consequence of the displacement of the otolith parallel to its own plane. This leads, naturally, to the definition of a sensation plane. A model is presented which defines this sensation plane. It assumes that the sacular otolith contribution is negligible, an assumption substantiated by correlation with experimental data by Sch\u00f6ne. Applying this model to perceived pitch angle, a simple equation is presented which relates the number of Gs and actual pitch angle to the perceived pitch angle. Experimental data are needed to develop perceived sensation curves for the sensation plane. The model implies that a state of confusion exists outside the realm of our prior experiences. Disorientation or motion sickness may be related to this confused state. In addition, new avenues of inquiry are introduced.", "contents": "Human tilt perception in a dynamic environment. This study examines the perception of tilt and linear acceleration as sensed through the utricular otolith of the vestibular system. Any sensation derived from the otolith is a consequence of the displacement of the otolith parallel to its own plane. This leads, naturally, to the definition of a sensation plane. A model is presented which defines this sensation plane. It assumes that the sacular otolith contribution is negligible, an assumption substantiated by correlation with experimental data by Sch\u00f6ne. Applying this model to perceived pitch angle, a simple equation is presented which relates the number of Gs and actual pitch angle to the perceived pitch angle. Experimental data are needed to develop perceived sensation curves for the sensation plane. The model implies that a state of confusion exists outside the realm of our prior experiences. Disorientation or motion sickness may be related to this confused state. In addition, new avenues of inquiry are introduced."}
{"id": "PMID:475709", "title": "Thermal strain resulting from protective clothing of an armored vehicle crew in warm conditions.", "content": "The purpose of the study is to define a method of evaluation of physiological strain resulting from protective garments worn in warm conditions by the armored vehicle crew. A technique is developed evaluating evaporative transfer through clothing by continuous weighing of the active man (accuracy +/- 3/g). An index is defined (Iw) as the ratio of steady-state evaporating rate in clothed conditions to steady-state evaporation of nude subject in the same conditions of work and heat stress. The Iw index is significantly related to physiological strain determined by increased body heat content and reduced tolerance time. The results are compared to other previous findings concerning evaporative transfer through clothing and physiological strain indexes. The technique shows that evaporation through heavy clothing is not negligible. It is suggested that usual static measurements using physical models underestimate the evaporative heat transfer through clothing layers.", "contents": "Thermal strain resulting from protective clothing of an armored vehicle crew in warm conditions. The purpose of the study is to define a method of evaluation of physiological strain resulting from protective garments worn in warm conditions by the armored vehicle crew. A technique is developed evaluating evaporative transfer through clothing by continuous weighing of the active man (accuracy +/- 3/g). An index is defined (Iw) as the ratio of steady-state evaporating rate in clothed conditions to steady-state evaporation of nude subject in the same conditions of work and heat stress. The Iw index is significantly related to physiological strain determined by increased body heat content and reduced tolerance time. The results are compared to other previous findings concerning evaporative transfer through clothing and physiological strain indexes. The technique shows that evaporation through heavy clothing is not negligible. It is suggested that usual static measurements using physical models underestimate the evaporative heat transfer through clothing layers."}
{"id": "PMID:475710", "title": "Physiologic and performance measurements in simulated airborne combined stress environments.", "content": "The Naval Air Development Center's Human Centrifuge was used to assess the effects of repeated exposures to a simulated Air Combat Maneuvering (ACM) environment on various measures of physiological function and psychomotor performance. The environment consisted of a realistic ACM profile that included associated noise, high speed/stall buffet conditions, and increased intra-cockpit temperatures. The effects of varying the subject's seatback angle, as a function of the environment, was also measured. Analysis of the various physiological and performance data revealed the following: 1) heart rate was negatively correlated with tracking accuracy under the combined stress conditions of this study, 2) test conditions that included acceleration and buffet resulted in a significant decrement in tracking accuracy, 3) the addition of increased intra-cocpit temperatures, when combined with acceleration and/or buffet, produced a significant decrease in tracking accuracy, 4) increasing the seatback angle from the vertical partially ameliorated the effects of the other stressors. Details of these findings, as well as results of various biochemical analyses, are discussed.", "contents": "Physiologic and performance measurements in simulated airborne combined stress environments. The Naval Air Development Center's Human Centrifuge was used to assess the effects of repeated exposures to a simulated Air Combat Maneuvering (ACM) environment on various measures of physiological function and psychomotor performance. The environment consisted of a realistic ACM profile that included associated noise, high speed/stall buffet conditions, and increased intra-cockpit temperatures. The effects of varying the subject's seatback angle, as a function of the environment, was also measured. Analysis of the various physiological and performance data revealed the following: 1) heart rate was negatively correlated with tracking accuracy under the combined stress conditions of this study, 2) test conditions that included acceleration and buffet resulted in a significant decrement in tracking accuracy, 3) the addition of increased intra-cocpit temperatures, when combined with acceleration and/or buffet, produced a significant decrease in tracking accuracy, 4) increasing the seatback angle from the vertical partially ameliorated the effects of the other stressors. Details of these findings, as well as results of various biochemical analyses, are discussed."}
{"id": "PMID:475711", "title": "Investigation of a simple, retrospective test for in-flight hyperventilation.", "content": "The experiment was designed to study the feasibility of using a single rebreathing estimate of mixed venous carbon dioxide tension (PvCO2) was a simple field test for hyperventilation in pilots. The results confirmed that the fall of end tidal carbon dioxide tension (P(ET)CO2) during hyperventilation and rise during recovery was exponential. The results also showed that the relationships between PvCO2 and P(ET)CO2 values during the unsteady states of carbon dioxide washout and accumulation may be described as a loop which encloses the theoretically derived line for the steady-state relationships. The deviation from the steady-state line appears on theoretical consideration to be directly proportional to carbon dioxide elimination rate, and indirectly proportional to cardiac output. Because of the exponential recovery, and because one value of PvCO2 could correspond to a range of values of P(ET)CO2, it is concluded that a field test for hyperventilation based on a single rebreathing estimate of PvCO2 would not be of value. The finding of a low value of PvCO2 would, however, be an indication that hyperventilation had taken place.", "contents": "Investigation of a simple, retrospective test for in-flight hyperventilation. The experiment was designed to study the feasibility of using a single rebreathing estimate of mixed venous carbon dioxide tension (PvCO2) was a simple field test for hyperventilation in pilots. The results confirmed that the fall of end tidal carbon dioxide tension (P(ET)CO2) during hyperventilation and rise during recovery was exponential. The results also showed that the relationships between PvCO2 and P(ET)CO2 values during the unsteady states of carbon dioxide washout and accumulation may be described as a loop which encloses the theoretically derived line for the steady-state relationships. The deviation from the steady-state line appears on theoretical consideration to be directly proportional to carbon dioxide elimination rate, and indirectly proportional to cardiac output. Because of the exponential recovery, and because one value of PvCO2 could correspond to a range of values of P(ET)CO2, it is concluded that a field test for hyperventilation based on a single rebreathing estimate of PvCO2 would not be of value. The finding of a low value of PvCO2 would, however, be an indication that hyperventilation had taken place."}
{"id": "PMID:475712", "title": "Motion sickness in cats: a symptom rating scale used in laboratory and flight tests.", "content": "The cat is proposed as a model for the study of motion and space sickness. Development of a scale for rating the motion sickness severity in the cat is described. The scale is used to evaluate an antimotion sickness drug, d-amphetamine plus scopolamine, and to determine whether it is possible to predict sickness susceptibility during parabolic flight, including zero-G maneuvers, from scores obtained during ground based trials.", "contents": "Motion sickness in cats: a symptom rating scale used in laboratory and flight tests. The cat is proposed as a model for the study of motion and space sickness. Development of a scale for rating the motion sickness severity in the cat is described. The scale is used to evaluate an antimotion sickness drug, d-amphetamine plus scopolamine, and to determine whether it is possible to predict sickness susceptibility during parabolic flight, including zero-G maneuvers, from scores obtained during ground based trials."}
{"id": "PMID:475713", "title": "Psychiatric education in aerospace medicine.", "content": "In order to facilitate curriculum design for psychiatric education in aerospace medicine, three groups of physicians were surveyed: experienced flight surgeons, student flight surgeons, and psychiatrists trained in aerospace medicine. Two measures were obtained: 1) an assessment of the usefulness of particular psychiatric skills and knowledge in the practice of aerospace medicine, and 2) a rating of the clinician's abilities in these areas. Results support the primary importance of interviewing and diagnostic skills. Student flight surgeons show deficiencies in the areas of administrative knowledge and in knowledge of the impact of the flyer's environment upon his mental status. These findings link psychiatry in aerospace medicine with the emphasis on understanding the man/environment interface and on the flight surgeon's organizational function of boundary control through decisions on fitness to fly.", "contents": "Psychiatric education in aerospace medicine. In order to facilitate curriculum design for psychiatric education in aerospace medicine, three groups of physicians were surveyed: experienced flight surgeons, student flight surgeons, and psychiatrists trained in aerospace medicine. Two measures were obtained: 1) an assessment of the usefulness of particular psychiatric skills and knowledge in the practice of aerospace medicine, and 2) a rating of the clinician's abilities in these areas. Results support the primary importance of interviewing and diagnostic skills. Student flight surgeons show deficiencies in the areas of administrative knowledge and in knowledge of the impact of the flyer's environment upon his mental status. These findings link psychiatry in aerospace medicine with the emphasis on understanding the man/environment interface and on the flight surgeon's organizational function of boundary control through decisions on fitness to fly."}
{"id": "PMID:475714", "title": "Frequency analysis of heart rate variability under flight conditions.", "content": "The factors causing heart rate variability (HRV) were investigated under various kinds of workloads and flight conditions. Frequency analysis of heart rate, and cross-correlation analysis with blood pressure and respiration, were performed by using the fast Fourier analyser. Consequently, the main frequency of HRV at rest consisted of 0.1 Hz and 0.25-0.3 Hz. These fluctuations disappeared under physical loads, but the value of 0.1 Hz at first increased under moderate mental load and thereafter decreased as the mental load increased. It was suggested that the behavior of the frequency 0.1 Hz under mental load was influenced partly by higher brain centers. For the actual flight, the frequency decreased under takeoff and landing, and remained as at rest during the gunnery and acrobatic training flight. It was concluded that the evaluation of workloads under flight conditions by using the analysis of the factors of HRV was very useful to improve a man-machine interface.", "contents": "Frequency analysis of heart rate variability under flight conditions. The factors causing heart rate variability (HRV) were investigated under various kinds of workloads and flight conditions. Frequency analysis of heart rate, and cross-correlation analysis with blood pressure and respiration, were performed by using the fast Fourier analyser. Consequently, the main frequency of HRV at rest consisted of 0.1 Hz and 0.25-0.3 Hz. These fluctuations disappeared under physical loads, but the value of 0.1 Hz at first increased under moderate mental load and thereafter decreased as the mental load increased. It was suggested that the behavior of the frequency 0.1 Hz under mental load was influenced partly by higher brain centers. For the actual flight, the frequency decreased under takeoff and landing, and remained as at rest during the gunnery and acrobatic training flight. It was concluded that the evaluation of workloads under flight conditions by using the analysis of the factors of HRV was very useful to improve a man-machine interface."}
{"id": "PMID:475715", "title": "Concurrent loss of consciousness and sino-atrial block during +Gz stress.", "content": "Current USAF fighter aircraft easily exceed human physiologic limits with their rapid onset of head-to-foot acceleration forces (+Gz). Sudden in-flight incapacitation caused by these increased +Gz forces could be disastrous with loss of materiel and human life. The physiologic mechanisms responsible for loss of consciousness (LOC) secondary to high +Gz must be fully understood so that maximum protection against it can be provided. An interesting case of an episode of LOC with concurrent sino-atrial block occurring during a relaxed rapid onset (1 G/s) centrifuge run is presented. The patient was undergoing flight medical evaluation for an episode of syncope, etiology unknown. An unusual characteristic of the patient was his high level of endurance training. The possibility of an excessive increase in vagal tone, developed by endurance training, is discussed as a probable etiology for this patient's prolonged time of incapacitation evidenced after +Gz-induced loss of consciousness.", "contents": "Concurrent loss of consciousness and sino-atrial block during +Gz stress. Current USAF fighter aircraft easily exceed human physiologic limits with their rapid onset of head-to-foot acceleration forces (+Gz). Sudden in-flight incapacitation caused by these increased +Gz forces could be disastrous with loss of materiel and human life. The physiologic mechanisms responsible for loss of consciousness (LOC) secondary to high +Gz must be fully understood so that maximum protection against it can be provided. An interesting case of an episode of LOC with concurrent sino-atrial block occurring during a relaxed rapid onset (1 G/s) centrifuge run is presented. The patient was undergoing flight medical evaluation for an episode of syncope, etiology unknown. An unusual characteristic of the patient was his high level of endurance training. The possibility of an excessive increase in vagal tone, developed by endurance training, is discussed as a probable etiology for this patient's prolonged time of incapacitation evidenced after +Gz-induced loss of consciousness."}
{"id": "PMID:475716", "title": "Fighter index of thermal stress (FITS): guidance for hot-weather aircraft operations.", "content": "Operation of fighter and trainer aircraft at low altitude in hot weather often involves significant heat stress on aircrews. Guidance for control of this stress and its adverse consequences has not heretofore been available. The Fighter Index of Thermal Stress (FITS) was derived from the Wet Bulb Globe Temperature (WBGT) using recent in-flight data on cockpit environments and assuming a fixed contribution from solar heating. The FITS table is entered with ground dry bulb temperature and dewpoint temperature, and yields an estimate of cockpit thermal stress. Caution and Danger Zones are designated on the table, based upon typical aircrew clothing, metabolic rate, and physiological status. Appropriate protective measures are recommended, including awareness of heat stress, limitations on ground operations, allowance of adequate recovery intervals, provision for fluid intake, and cancellation of flights under severe conditions. Possible applications of FITS are discussed together with its potential impact on flight operations at 30 USAF bases.", "contents": "Fighter index of thermal stress (FITS): guidance for hot-weather aircraft operations. Operation of fighter and trainer aircraft at low altitude in hot weather often involves significant heat stress on aircrews. Guidance for control of this stress and its adverse consequences has not heretofore been available. The Fighter Index of Thermal Stress (FITS) was derived from the Wet Bulb Globe Temperature (WBGT) using recent in-flight data on cockpit environments and assuming a fixed contribution from solar heating. The FITS table is entered with ground dry bulb temperature and dewpoint temperature, and yields an estimate of cockpit thermal stress. Caution and Danger Zones are designated on the table, based upon typical aircrew clothing, metabolic rate, and physiological status. Appropriate protective measures are recommended, including awareness of heat stress, limitations on ground operations, allowance of adequate recovery intervals, provision for fluid intake, and cancellation of flights under severe conditions. Possible applications of FITS are discussed together with its potential impact on flight operations at 30 USAF bases."}
{"id": "PMID:475725", "title": "[An indirect proof of stretch-induced Ca++ release from the sarcoplasmic reticulum in glycerinated skeletal and heart muscle preparations (author's transl)].", "content": "The existence of still functioning vesicles of the sarcoplasmic reticulum (SR) in glycerinated rabbit psoas muscle fibers and cat myocardium is demonstrated by the use of activation (force development and shortening) of the contractile system as an indicator for \"sarcoplasmic\", free Ca++ concentration and by electron microscopic studies. Furthermore, the conditions for Ca++ release and uptake by the SR vesicles were analyzed. (1) As indicated by electron microscopic studies, glycerinated skeletal and heart muscle preparations contain SR vesicles (diameter 0.1--0.2 mu). Detergent treatment as used by Julian (1971) causes neither morphological change in the contractile system nor in the SR vesicles. (2) The delay in tension development of glycerinated preparations after an increase in free Ca++ concentration of the bathing medium is demonstrated to be caused at least partly by Ca++ uptake in these SR vesicles. (3) The SR vesicles of glycerinated preparations are able to accumulate Ca++ just at concentrations which are subthreshold for tension development. The accumulated Ca++ can be detected by a transient force development resulting from abolishment of the Ca++ storage capacity of the SR vesicles caused by high concentrations of detergents. (4) Ca++-induced Ca++ release is also demonstrated in glycerinated preparations. After Ca++ loading this release mechanism occurs at Ca++ concentrations in the bathing medium starting at about 10(-7,48) Mol/l both in skeletal and heart muscle preparations. (5) Quick stretch of Ca++-loaded, relaxed preparations caused local contractions of the myofibrils in both single fibers and fiber bundles of glycerinated rabbit psoas muscle. Skeletal fiber bundles and cat myocardium show a transient tension development parallel to these local contractions. These effects are demonstrated to be due to a stretch-induced Ca++ release from the SR vesicles. (6) Length-controlled quick stretches or quick releases of partly activated preparations cause sinusoidal tension oscillations superimposed on the well-known, delayed active tension development. In contrast, fully activated preparations do not show superimposed oscillations in tension. Force-controlled quick stretches or quick releases induce equivalent oscillations in length which are more distinct than the tension oscillations, however. These oscillations are also demonstrated to be due to stretch or release-induced changes in the Ca++ fluxes between SR vesicles and the \"sarcoplasm\". (7) This stretch induced release of Ca++ from the SR could be one reason for the multiple-phase course of active and passive isometric tension development in vivo following quick changes in length, and could also partly explain the prestretch dependence of tension development which cannot be explained from the degree of overlapping of the contractile filaments alone...", "contents": "[An indirect proof of stretch-induced Ca++ release from the sarcoplasmic reticulum in glycerinated skeletal and heart muscle preparations (author's transl)]. The existence of still functioning vesicles of the sarcoplasmic reticulum (SR) in glycerinated rabbit psoas muscle fibers and cat myocardium is demonstrated by the use of activation (force development and shortening) of the contractile system as an indicator for \"sarcoplasmic\", free Ca++ concentration and by electron microscopic studies. Furthermore, the conditions for Ca++ release and uptake by the SR vesicles were analyzed. (1) As indicated by electron microscopic studies, glycerinated skeletal and heart muscle preparations contain SR vesicles (diameter 0.1--0.2 mu). Detergent treatment as used by Julian (1971) causes neither morphological change in the contractile system nor in the SR vesicles. (2) The delay in tension development of glycerinated preparations after an increase in free Ca++ concentration of the bathing medium is demonstrated to be caused at least partly by Ca++ uptake in these SR vesicles. (3) The SR vesicles of glycerinated preparations are able to accumulate Ca++ just at concentrations which are subthreshold for tension development. The accumulated Ca++ can be detected by a transient force development resulting from abolishment of the Ca++ storage capacity of the SR vesicles caused by high concentrations of detergents. (4) Ca++-induced Ca++ release is also demonstrated in glycerinated preparations. After Ca++ loading this release mechanism occurs at Ca++ concentrations in the bathing medium starting at about 10(-7,48) Mol/l both in skeletal and heart muscle preparations. (5) Quick stretch of Ca++-loaded, relaxed preparations caused local contractions of the myofibrils in both single fibers and fiber bundles of glycerinated rabbit psoas muscle. Skeletal fiber bundles and cat myocardium show a transient tension development parallel to these local contractions. These effects are demonstrated to be due to a stretch-induced Ca++ release from the SR vesicles. (6) Length-controlled quick stretches or quick releases of partly activated preparations cause sinusoidal tension oscillations superimposed on the well-known, delayed active tension development. In contrast, fully activated preparations do not show superimposed oscillations in tension. Force-controlled quick stretches or quick releases induce equivalent oscillations in length which are more distinct than the tension oscillations, however. These oscillations are also demonstrated to be due to stretch or release-induced changes in the Ca++ fluxes between SR vesicles and the \"sarcoplasm\". (7) This stretch induced release of Ca++ from the SR could be one reason for the multiple-phase course of active and passive isometric tension development in vivo following quick changes in length, and could also partly explain the prestretch dependence of tension development which cannot be explained from the degree of overlapping of the contractile filaments alone..."}
{"id": "PMID:475724", "title": "Effects of adaptation to high altitude hypoxia on the contractile function and adrenoreactivity of the heart.", "content": "Effects of adaptation to mountain altitude of 3200 m on the contractile function and adrenoreactivity of the heart in rats have been investigated. It was shown that adaptation to altitude increased the contractile force and contraction and relaxation velocities of the left ventricle as compared to the controls. Simultaneously, a significant increase in cardiac response to noradrenaline developed in the course of adaptation. The increased response was accompanied by its more rapid disappearance. These changes may be explained by the increase in the activity of myocardial adenylate cyclase and phosphodiesterase. It was also shown that the decrease in cardiac function produced by cardiac denervation was less pronounced in adapted rats. This fact may be explained by increased effectiveness of cardiac autoregulatory mechanism. The comparison of these results with the data of other investigators suggests that in well adapted animals the strength of interaction between the levels of the regulatory hierarchy of the whole organism changes, i.e., the capacity of autoregulatory cell mechanisms and their reactivity to neurohumoral stimuli increase. As a result, control of the organism's reactions by higher levels of the regulatory system is more economical in adapted animals.", "contents": "Effects of adaptation to high altitude hypoxia on the contractile function and adrenoreactivity of the heart. Effects of adaptation to mountain altitude of 3200 m on the contractile function and adrenoreactivity of the heart in rats have been investigated. It was shown that adaptation to altitude increased the contractile force and contraction and relaxation velocities of the left ventricle as compared to the controls. Simultaneously, a significant increase in cardiac response to noradrenaline developed in the course of adaptation. The increased response was accompanied by its more rapid disappearance. These changes may be explained by the increase in the activity of myocardial adenylate cyclase and phosphodiesterase. It was also shown that the decrease in cardiac function produced by cardiac denervation was less pronounced in adapted rats. This fact may be explained by increased effectiveness of cardiac autoregulatory mechanism. The comparison of these results with the data of other investigators suggests that in well adapted animals the strength of interaction between the levels of the regulatory hierarchy of the whole organism changes, i.e., the capacity of autoregulatory cell mechanisms and their reactivity to neurohumoral stimuli increase. As a result, control of the organism's reactions by higher levels of the regulatory system is more economical in adapted animals."}
{"id": "PMID:475727", "title": "Experimental occlusion of two small coronary arteries in the same heart. A new validation method for infarct size manipulation.", "content": "A method for the evaluation of interventions aimed at manipulation of infarct size is described. This method has 2 advantages over other methods: 1. Two small-to-medium sized coronary branches of the same heart are occluded and reperfused in sequence. Thereby a \"control-infarct\" can be compared with a \"test-infarct\", both within the same heart. 2. The amount of infarcted tissue in both areas is quantitated by using the p-NBT method. A condition of the method is that the areas of perfusion of both arteries are equal. Infarct size is always expressed as a percentage of the perfusion area of the occluded artery. Validation experiments with simultaneous occlusion and reperfusion of both arteries resulted in infarcts of equal size. Validation experiments with regard to the p-NBT method showed that reperfusion accelerates the washout of dehydrogenases from infarcted tissue which allows early and precise diagnosis of infarcts after relatively short occlusion- and reperfusion periods.", "contents": "Experimental occlusion of two small coronary arteries in the same heart. A new validation method for infarct size manipulation. A method for the evaluation of interventions aimed at manipulation of infarct size is described. This method has 2 advantages over other methods: 1. Two small-to-medium sized coronary branches of the same heart are occluded and reperfused in sequence. Thereby a \"control-infarct\" can be compared with a \"test-infarct\", both within the same heart. 2. The amount of infarcted tissue in both areas is quantitated by using the p-NBT method. A condition of the method is that the areas of perfusion of both arteries are equal. Infarct size is always expressed as a percentage of the perfusion area of the occluded artery. Validation experiments with simultaneous occlusion and reperfusion of both arteries resulted in infarcts of equal size. Validation experiments with regard to the p-NBT method showed that reperfusion accelerates the washout of dehydrogenases from infarcted tissue which allows early and precise diagnosis of infarcts after relatively short occlusion- and reperfusion periods."}
{"id": "PMID:475730", "title": "Myofibroblasts in subepicardium after local cold injury.", "content": "Rat hearts were exposed and injured locally by direct cryoapplication. The hearts were examined 2, 4, 20 and 48 hours, 7 and 12 days later. In the first 48 hours mast cell and polymorphonuclear (mainly eosinophilic) granulocyte infiltration was followed by proliferation of mononuclear phagocytic and fibroblastic cells and new capillaries. The underlying muscle showed freeze-thaw-rigor damage, segmental intramyofiber necrosis, phagocytosis and focal regeneration. After 7 days numerous myofibroblasts appeared and they were intimately related to myofibers undergoing degeneration and repair. After 12 days there was increased fibrosis while mesothelial covering cells of the epicardium and myofibers returned to normal. It is suggested that amines released from mast cells are involved in the formation of myofibroblasts.", "contents": "Myofibroblasts in subepicardium after local cold injury. Rat hearts were exposed and injured locally by direct cryoapplication. The hearts were examined 2, 4, 20 and 48 hours, 7 and 12 days later. In the first 48 hours mast cell and polymorphonuclear (mainly eosinophilic) granulocyte infiltration was followed by proliferation of mononuclear phagocytic and fibroblastic cells and new capillaries. The underlying muscle showed freeze-thaw-rigor damage, segmental intramyofiber necrosis, phagocytosis and focal regeneration. After 7 days numerous myofibroblasts appeared and they were intimately related to myofibers undergoing degeneration and repair. After 12 days there was increased fibrosis while mesothelial covering cells of the epicardium and myofibers returned to normal. It is suggested that amines released from mast cells are involved in the formation of myofibroblasts."}
{"id": "PMID:475733", "title": "Experimental validation of methods for the measurement of coronary sinus blood flow in man.", "content": "The measurement accuracy of clinically applicable methods for blood flow measurement in coronary sinus -- continuous local thermodilution (LTD), differential pressure (DP), ultrasonic Doppler (US) and the electromagnetic flow measurement method (EMF) -- was examined in 15 anaesthetized closed chest dogs with left ventricle weights between 150 and 200 g. The LTD, DP, US and the EMF were examined in each experiment in the two following arrangements. 1. In coronary sinus -- left jugular vein by-pass: This arrangement allowed four reference methods for measurement of coronary sinus blood flow (CBF). 2. In \"clinical\" position, without by-pass, which allowed two reference methods for CBF measurements. The results on the measurement accuracy of the LTD, DP and US are, depending on the measurement arrangement, contradictory. In the by-pass arrangement 1 there was observed a good agreement of the LTD, DP and US CBF values with the reference values. In the \"clinical\" position, without by-pass 2 the measurement accuracy of LTD was not sufficient for exact measurement of CBF and derived parameters. The examined velocity tip flow probes (US, DP) gave no correlation with the reference methods. US and DP are even for semiquantitative estimation of CBF unsuitable. The EMF tip flow probe was for the CBF measurement unsuitable, because of disturbance by the electrical activity of myocardium.", "contents": "Experimental validation of methods for the measurement of coronary sinus blood flow in man. The measurement accuracy of clinically applicable methods for blood flow measurement in coronary sinus -- continuous local thermodilution (LTD), differential pressure (DP), ultrasonic Doppler (US) and the electromagnetic flow measurement method (EMF) -- was examined in 15 anaesthetized closed chest dogs with left ventricle weights between 150 and 200 g. The LTD, DP, US and the EMF were examined in each experiment in the two following arrangements. 1. In coronary sinus -- left jugular vein by-pass: This arrangement allowed four reference methods for measurement of coronary sinus blood flow (CBF). 2. In \"clinical\" position, without by-pass, which allowed two reference methods for CBF measurements. The results on the measurement accuracy of the LTD, DP and US are, depending on the measurement arrangement, contradictory. In the by-pass arrangement 1 there was observed a good agreement of the LTD, DP and US CBF values with the reference values. In the \"clinical\" position, without by-pass 2 the measurement accuracy of LTD was not sufficient for exact measurement of CBF and derived parameters. The examined velocity tip flow probes (US, DP) gave no correlation with the reference methods. US and DP are even for semiquantitative estimation of CBF unsuitable. The EMF tip flow probe was for the CBF measurement unsuitable, because of disturbance by the electrical activity of myocardium."}
{"id": "PMID:475731", "title": "Coronary reactive hyperaemia and coronary dilator action of adenosine during intracoronary infusion of angiotensin II.", "content": "Investigations were carried out in chloralose-anesthetized, thoracotomized dogs. The effects of an intracoronary infusion of angiotensin II (3 ng/ml coronary blood) on reactive hyperaemic blood flow and adenosine-induced coronary vasodilation were established. Coronary artery occlusions were performed over periods of 5, 10 and 25 heart beats and adenosine was injected intracoronarily at dosages of 2, 4, and 8 microgram/kg body weight. The vasodilator effect of adenosine and reactive hyperaemia after 10 and 25 heart beats were both significantly diminished by angiotensin II. By contrast, reactive hyperaemia after coronary artery occlusion lasting 5 heart beats remained unchanged. The present results obtained with angiotensin II further confirm that adenosine is only partially involved in mediating reactive hyperaemia and that this involvement takes place only after sufficiently long periods of coronary artery occlusion.", "contents": "Coronary reactive hyperaemia and coronary dilator action of adenosine during intracoronary infusion of angiotensin II. Investigations were carried out in chloralose-anesthetized, thoracotomized dogs. The effects of an intracoronary infusion of angiotensin II (3 ng/ml coronary blood) on reactive hyperaemic blood flow and adenosine-induced coronary vasodilation were established. Coronary artery occlusions were performed over periods of 5, 10 and 25 heart beats and adenosine was injected intracoronarily at dosages of 2, 4, and 8 microgram/kg body weight. The vasodilator effect of adenosine and reactive hyperaemia after 10 and 25 heart beats were both significantly diminished by angiotensin II. By contrast, reactive hyperaemia after coronary artery occlusion lasting 5 heart beats remained unchanged. The present results obtained with angiotensin II further confirm that adenosine is only partially involved in mediating reactive hyperaemia and that this involvement takes place only after sufficiently long periods of coronary artery occlusion."}
{"id": "PMID:475732", "title": "Vagal control of coronary blood flow in dogs.", "content": "The experiments were performed on open-chest mongrel dogs, the hearts being paced at a constant rate. Both vagi were cut in the neck and the peripheral ends were stimulated with frequencies of 1, 5, 10, 15, 20, 25, 30, 35, 40 and 50 cycles per sec (30 sec, 8 V, 2 msec). Adrenergic beta-receptors were blocked with propranolol and adrenergic alpha-receptors with dihydroergotamine. Coronary flow was measured with electromagnetic flowmeters on the descending branch of the left coronary artery. A significant increase in coronary flow was observed with rising frequency of stimulation. This increase was 15 percent with 30 Hz and decreased with higher frequencies. The total coronary resistance reached its lowest level at the frequency of 30 cycles per sec. The results clearly indicate the parasympathetic control of myocardial blood flow.", "contents": "Vagal control of coronary blood flow in dogs. The experiments were performed on open-chest mongrel dogs, the hearts being paced at a constant rate. Both vagi were cut in the neck and the peripheral ends were stimulated with frequencies of 1, 5, 10, 15, 20, 25, 30, 35, 40 and 50 cycles per sec (30 sec, 8 V, 2 msec). Adrenergic beta-receptors were blocked with propranolol and adrenergic alpha-receptors with dihydroergotamine. Coronary flow was measured with electromagnetic flowmeters on the descending branch of the left coronary artery. A significant increase in coronary flow was observed with rising frequency of stimulation. This increase was 15 percent with 30 Hz and decreased with higher frequencies. The total coronary resistance reached its lowest level at the frequency of 30 cycles per sec. The results clearly indicate the parasympathetic control of myocardial blood flow."}
{"id": "PMID:475736", "title": "Long term effects of the anorectic agent fenfluramine alone and in combination with aminorex on pulmonary and systemic circulation in the pig.", "content": "We have investigated the effects of chronic oral administration of two anorectic substances, fenfluramine and aminorex, especially on the pulmonary circulation in young pigs with one ligated pulmonary artery. The pulmonary vascular reactivity was tested by alveolar hypoxia, alveolar hyperoxia and infusion of prostaglandin F2ALPHA. No elevation of pulmonary arterial pressures or resistances were found due to the intake of fenfluramine or aminorex over a three month period. The responses to the vasoconstrictor stimuli, hypoxia and prostaglandin F2alpha, and to the vasodilator stimulus, hyperoxia, were equal and not augmented in the drug groups. Fenfluramine or aminorex could therefore could therefore not be shown to have an adverse effect on the pulmonary circulation or on the reactivity of the pulmonary vascular bed in the pig, but fenfluramine elevated systemic arterial pressure.", "contents": "Long term effects of the anorectic agent fenfluramine alone and in combination with aminorex on pulmonary and systemic circulation in the pig. We have investigated the effects of chronic oral administration of two anorectic substances, fenfluramine and aminorex, especially on the pulmonary circulation in young pigs with one ligated pulmonary artery. The pulmonary vascular reactivity was tested by alveolar hypoxia, alveolar hyperoxia and infusion of prostaglandin F2ALPHA. No elevation of pulmonary arterial pressures or resistances were found due to the intake of fenfluramine or aminorex over a three month period. The responses to the vasoconstrictor stimuli, hypoxia and prostaglandin F2alpha, and to the vasodilator stimulus, hyperoxia, were equal and not augmented in the drug groups. Fenfluramine or aminorex could therefore could therefore not be shown to have an adverse effect on the pulmonary circulation or on the reactivity of the pulmonary vascular bed in the pig, but fenfluramine elevated systemic arterial pressure."}
{"id": "PMID:475737", "title": "[The adaptation of AV-nodal conduction time on gliding increase and decrease of atrial frequency before and after autonomic blockade (author's transl)].", "content": "In 19 patients with healthy AV-nodes the adaptation of the intranodal conduction time (A-H time) to gliding increase and decrease in atrial frequency and to the blockade of the autonomic nervous system was investigated using His bundle electrograms. The measurements were performed during right atrial stimulation with three frequencies, each with a duration of one minute, before and after blockade of the parasympathetic (8 pat.; 1 mg atropine i.v.) and the sympathetic (11 pat.; 0.4 mg Visken i.v.) nervous system. Gliding increase and decrease in atrial frequency results in a staircase pattern of A-H-adaptation in 18 of the patients. The height of the steps was identical in both phases of stimulation in each individual patient. One patient showed functional dissociation of intranodal conduction which was different during increase and decrease of atrial frequency. With parasympathetic blockade the staircase behavior of the A-H time basically remained unchanged with the exception of shorter A-H intervals resulting in lower steps. Atropine abolished the functional dissociation of intranodal conduction; thus the drug might prevent reentrytachycardias due to functional dissociation in the AV-node. Sympathetic blockade lengthens the intranodal conduction time; thus shifting the staircase pattern of the A-H time to higher levels. The results are discussed with respect to the electrophysiological characteristics of AV nodal cells as slow response fibers, and to the changes caused by atrial stimulation, acetylcholine and adrenaline.", "contents": "[The adaptation of AV-nodal conduction time on gliding increase and decrease of atrial frequency before and after autonomic blockade (author's transl)]. In 19 patients with healthy AV-nodes the adaptation of the intranodal conduction time (A-H time) to gliding increase and decrease in atrial frequency and to the blockade of the autonomic nervous system was investigated using His bundle electrograms. The measurements were performed during right atrial stimulation with three frequencies, each with a duration of one minute, before and after blockade of the parasympathetic (8 pat.; 1 mg atropine i.v.) and the sympathetic (11 pat.; 0.4 mg Visken i.v.) nervous system. Gliding increase and decrease in atrial frequency results in a staircase pattern of A-H-adaptation in 18 of the patients. The height of the steps was identical in both phases of stimulation in each individual patient. One patient showed functional dissociation of intranodal conduction which was different during increase and decrease of atrial frequency. With parasympathetic blockade the staircase behavior of the A-H time basically remained unchanged with the exception of shorter A-H intervals resulting in lower steps. Atropine abolished the functional dissociation of intranodal conduction; thus the drug might prevent reentrytachycardias due to functional dissociation in the AV-node. Sympathetic blockade lengthens the intranodal conduction time; thus shifting the staircase pattern of the A-H time to higher levels. The results are discussed with respect to the electrophysiological characteristics of AV nodal cells as slow response fibers, and to the changes caused by atrial stimulation, acetylcholine and adrenaline."}
{"id": "PMID:475735", "title": "Substrate effects on mitochondrial function and tissue lipids in low-flow hypoxia of isolated perfused rat hearts.", "content": "A possible causal relationship between tissue FFA contents and the depression in mitochondrial oxidative phosphorylation in myocardial ischaemia has been suggested. To test this hypothesis, the effects of different substrates added to the perfusates of hypoxic, low-flow perfused hearts were examined on oxidative phosphorylation catalysed by mitochondria isolated from such tissue. In an additional series of experiments tissue neutral glyceride and FFA levels were analysed and correlated with changes in mitochondrial function. Mitochondria isolated from hearts with a high tissue FFA content exhibited the lowest ADP/O ratios, RCI and QO2 values. On the other hand, mitochondria isolated from hearts with reduced FFA contents, performed significantly better with respect to these parameters of mitochondrial function studied.", "contents": "Substrate effects on mitochondrial function and tissue lipids in low-flow hypoxia of isolated perfused rat hearts. A possible causal relationship between tissue FFA contents and the depression in mitochondrial oxidative phosphorylation in myocardial ischaemia has been suggested. To test this hypothesis, the effects of different substrates added to the perfusates of hypoxic, low-flow perfused hearts were examined on oxidative phosphorylation catalysed by mitochondria isolated from such tissue. In an additional series of experiments tissue neutral glyceride and FFA levels were analysed and correlated with changes in mitochondrial function. Mitochondria isolated from hearts with a high tissue FFA content exhibited the lowest ADP/O ratios, RCI and QO2 values. On the other hand, mitochondria isolated from hearts with reduced FFA contents, performed significantly better with respect to these parameters of mitochondrial function studied."}
{"id": "PMID:475748", "title": "Mass-spectral identification and purification of phycoerythrobilin and phycocyanobilin.", "content": "The bile-pigment chromophores of C-phycoerythrin (phycoerythrobilin) and C-phycocyanin (phycocyanobilin) were cleaved from their respective proteins with boiling methanol or butan-1-ol. They were purified as dicarboxylic acids by preparative reverse-phase liquid chromatography. Each pigment existed in two principal forms, which were characterized by using 20 pmol samples by proton-transfer chemical-ionization mass spectroscopy. These two principal forms were isomeric species, and all had protonated parent molecular ions with m/e 587, corresponding to a molecular weight of 586.", "contents": "Mass-spectral identification and purification of phycoerythrobilin and phycocyanobilin. The bile-pigment chromophores of C-phycoerythrin (phycoerythrobilin) and C-phycocyanin (phycocyanobilin) were cleaved from their respective proteins with boiling methanol or butan-1-ol. They were purified as dicarboxylic acids by preparative reverse-phase liquid chromatography. Each pigment existed in two principal forms, which were characterized by using 20 pmol samples by proton-transfer chemical-ionization mass spectroscopy. These two principal forms were isomeric species, and all had protonated parent molecular ions with m/e 587, corresponding to a molecular weight of 586."}
{"id": "PMID:475749", "title": "Characterization of lipid-protein interactions in acetylcholinesterase lipoprotein extracted from bovine erythrocytes.", "content": "Acetylcholinesterase was released from bovine erythrocytes in hypo-osmotic sodium phosphate buffer. Initially, about 30% of the enzyme was released in a soluble lipoprotein form, and further incubation resulted in the progressive release of the enzyme in a particulate form. Solubilization of the acetylcholinesterase in the particulate fraction with Lubrol WX (2 mg/ml) resulted in the loss of all lipids except a non-exchangeable fraction identified as cardiolipin. Addition of a mixture of erythrocyte phospholipids to the soluble forms and to the Lubrol WX-solubilized enzyme resulted in the formation of particulate forms of the enzyme with increased partial specific volume and Stokes radius, and a break in the Arrhenius plot of the enzyme activity around 20 degrees C. The break in the Arrhenius plot was abolished by treatment of a soluble enzyme preparation with 1.8 M salt (NaCl) in phosphate buffer, conditions that allowed the extraction of cardiolipin from the enzyme by chloroform/methanol. Failure of the high-salt treatment to decrease the Stokes radius made it unlikely that the bound cardiolipin formed a boundary layer or annulus around the protein. It is suggested that cardiolipin is bound to the core of the dimeric protein structure, thereby controlling the acetylcholinesterase activity.", "contents": "Characterization of lipid-protein interactions in acetylcholinesterase lipoprotein extracted from bovine erythrocytes. Acetylcholinesterase was released from bovine erythrocytes in hypo-osmotic sodium phosphate buffer. Initially, about 30% of the enzyme was released in a soluble lipoprotein form, and further incubation resulted in the progressive release of the enzyme in a particulate form. Solubilization of the acetylcholinesterase in the particulate fraction with Lubrol WX (2 mg/ml) resulted in the loss of all lipids except a non-exchangeable fraction identified as cardiolipin. Addition of a mixture of erythrocyte phospholipids to the soluble forms and to the Lubrol WX-solubilized enzyme resulted in the formation of particulate forms of the enzyme with increased partial specific volume and Stokes radius, and a break in the Arrhenius plot of the enzyme activity around 20 degrees C. The break in the Arrhenius plot was abolished by treatment of a soluble enzyme preparation with 1.8 M salt (NaCl) in phosphate buffer, conditions that allowed the extraction of cardiolipin from the enzyme by chloroform/methanol. Failure of the high-salt treatment to decrease the Stokes radius made it unlikely that the bound cardiolipin formed a boundary layer or annulus around the protein. It is suggested that cardiolipin is bound to the core of the dimeric protein structure, thereby controlling the acetylcholinesterase activity."}
{"id": "PMID:475750", "title": "4-pyridylmethyl, a thiol-protecting group suitable for the partial synthesis of proteins.", "content": "Cysteine is converted into S-4-pyridylmethylcysteine [Gosden, Stevenson & Young (1972) J. Chem. Soc. Chem Commun. 1123-1124] by 4-pyridylmethyl chloride in aqueous propanol at pH 8.3. The derivative is stable to the conditions of total acid hydrolysis. Reduction and alkylation of bovine insulin (pH 8.3, aq. 50% propanol) gives fully S-substituted derivatives in excellent yields. The S-pyridylmethylated A- and B-chains of insulin were separated by gel filtration: each of them has good solubility properties. The pyridylmethyl group is cleaved by electrolysis in a dilute acid medium, pH 2.6, to give reduced chains. They can be recombined to give insulin in the same yield and with the same degree of biological activity as chains which had not been subjected to the protection and de-protection steps. The results indicate that pyridylmethyl satisfactorily meets requirements for partial synthesis and suggest that it warrants more general use.", "contents": "4-pyridylmethyl, a thiol-protecting group suitable for the partial synthesis of proteins. Cysteine is converted into S-4-pyridylmethylcysteine [Gosden, Stevenson & Young (1972) J. Chem. Soc. Chem Commun. 1123-1124] by 4-pyridylmethyl chloride in aqueous propanol at pH 8.3. The derivative is stable to the conditions of total acid hydrolysis. Reduction and alkylation of bovine insulin (pH 8.3, aq. 50% propanol) gives fully S-substituted derivatives in excellent yields. The S-pyridylmethylated A- and B-chains of insulin were separated by gel filtration: each of them has good solubility properties. The pyridylmethyl group is cleaved by electrolysis in a dilute acid medium, pH 2.6, to give reduced chains. They can be recombined to give insulin in the same yield and with the same degree of biological activity as chains which had not been subjected to the protection and de-protection steps. The results indicate that pyridylmethyl satisfactorily meets requirements for partial synthesis and suggest that it warrants more general use."}
{"id": "PMID:475751", "title": "2-Sulphobenzyl, a new solubilizing and reversible protecting group for cysteine in proteins. Its scope and limitations.", "content": "S-2-Sulphobenzylcysteine and S-2-(sulphomethyl)benzylcysteine are prepared by alkylation of cysteine with omega-toluenesultone and 2,3-benzo-1,4-butanesultone respectively. Owing to the presence of the sulphonic acid group, these protected cysteine derivatives are extremely water-soluble and are stable to acid hydrolysis. The groups can be removed by treatment with sodium in liquid NH3. Reduction with tributylphosphine and simultaneous alkylation of insulin with toluenesultone under mild conditions (pH 8.3, aq. 50% propanol) gives the fully S-substituted derivatives in excellent yield; they can be separated by isoelectric precipitation of the S-sulphobenzylated B-chain. Treatment of the latter with sodium in liquid NH3 led simultaneously to the removal of the protecting groups and to the well-documented cleavage at the threonine-proline bond which can be prevented by addition of sodium amide. When deprotected A-chain was recombined with B-chain, insulin was isolated in the same yield and with the same degree of biological activity as that in the control experiment.", "contents": "2-Sulphobenzyl, a new solubilizing and reversible protecting group for cysteine in proteins. Its scope and limitations. S-2-Sulphobenzylcysteine and S-2-(sulphomethyl)benzylcysteine are prepared by alkylation of cysteine with omega-toluenesultone and 2,3-benzo-1,4-butanesultone respectively. Owing to the presence of the sulphonic acid group, these protected cysteine derivatives are extremely water-soluble and are stable to acid hydrolysis. The groups can be removed by treatment with sodium in liquid NH3. Reduction with tributylphosphine and simultaneous alkylation of insulin with toluenesultone under mild conditions (pH 8.3, aq. 50% propanol) gives the fully S-substituted derivatives in excellent yield; they can be separated by isoelectric precipitation of the S-sulphobenzylated B-chain. Treatment of the latter with sodium in liquid NH3 led simultaneously to the removal of the protecting groups and to the well-documented cleavage at the threonine-proline bond which can be prevented by addition of sodium amide. When deprotected A-chain was recombined with B-chain, insulin was isolated in the same yield and with the same degree of biological activity as that in the control experiment."}
{"id": "PMID:475752", "title": "Kinetic behaviour of acid phosphatase-albumin co-polymers in homogeneous phase and under gel-immobilized conditions.", "content": "1. An analysis of the kinetic behaviour of immobilized acid phosphatase (EC 3.1.3.2) layers, gelled on the active surface of an ultrafiltration membrane, was carried out. 2. Two possible forms of such immobilized-enzyme systems were dealt with, namely enzyme-polyalbumin co-gelation through an ultrafiltration process, and enzyme co-polymerization to the same albumin polymers and subsequent gelation. 3. A preliminary analysis was also performed on both the corresponding homogeneous-phase (soluble systems to provide reference kinetics. 4. The main conclusions drawn are: (i) the enzyme-albumin co-polymers show a decrease in specific activity compared with the corresponding free enzyme in both soluble and immobilized forms; (ii) in the homogeneous phase a slight increase in the apparent Michaelis constant was measured for the co-polymerized enzyme compared with the free one, which suggests a decrease in affinity towards substrate; (iii) the activation energy in the immobilized phase is halved, compared with that in the homogeneous phase, which indicates that the combined mass-transfer/reaction step is rate-controlling.", "contents": "Kinetic behaviour of acid phosphatase-albumin co-polymers in homogeneous phase and under gel-immobilized conditions. 1. An analysis of the kinetic behaviour of immobilized acid phosphatase (EC 3.1.3.2) layers, gelled on the active surface of an ultrafiltration membrane, was carried out. 2. Two possible forms of such immobilized-enzyme systems were dealt with, namely enzyme-polyalbumin co-gelation through an ultrafiltration process, and enzyme co-polymerization to the same albumin polymers and subsequent gelation. 3. A preliminary analysis was also performed on both the corresponding homogeneous-phase (soluble systems to provide reference kinetics. 4. The main conclusions drawn are: (i) the enzyme-albumin co-polymers show a decrease in specific activity compared with the corresponding free enzyme in both soluble and immobilized forms; (ii) in the homogeneous phase a slight increase in the apparent Michaelis constant was measured for the co-polymerized enzyme compared with the free one, which suggests a decrease in affinity towards substrate; (iii) the activation energy in the immobilized phase is halved, compared with that in the homogeneous phase, which indicates that the combined mass-transfer/reaction step is rate-controlling."}
{"id": "PMID:475753", "title": "The effect of restricted hydration on the rate of reaction of glucose 6-phosphate dehydrogenase, phosphoglucose isomerase, hexokinase and fumarase.", "content": "A method is described for the measurement of enzyme activity under xeric conditions. The reaction mixtures had water contents ranging between 0.1 and 0.6g/g of reaction mixture. For glucose 6-phosphate dehydrogenase, hexokinase and fumarase, enzyme activity became detectable (about 0.05% of the fully hydrated rate) when the water content was about 0.2g/g of reaction mixture, and for phosphoglucose isomerase, around 0.15g/g of reaction mixture. With the water content raised to 0.3g/g of reaction mixture the reaction rates were only increased to 0.1-3% of the fully hydrated rate. When the combined rates for phosphoglucose isomerase and glucose 6-phosphate dehydrogenase were measured, reasonable agreement was found between the experimental data and those calculated from the individual experimentally determined rates on the assumption that diffusion was not further limiting. A method was devised for measuring the diffusion coefficients of low-molecular-weight substances in solutions having low water contents. The diffusion coefficients of riboflavin in sorbitol solution decreased by about 100-fold when the water content of the latter was reduced from 3 to 0.25g/g of sorbitol. It is concluded that to detect enzyme activity a certain minimal amount of water is required and that above this minimum the rate is still restricted by diffusion limitation. The relevance of the results to the physical state of water in reaction mixtures and to metabolism in seeds and spores in xeric conditions is discussed.", "contents": "The effect of restricted hydration on the rate of reaction of glucose 6-phosphate dehydrogenase, phosphoglucose isomerase, hexokinase and fumarase. A method is described for the measurement of enzyme activity under xeric conditions. The reaction mixtures had water contents ranging between 0.1 and 0.6g/g of reaction mixture. For glucose 6-phosphate dehydrogenase, hexokinase and fumarase, enzyme activity became detectable (about 0.05% of the fully hydrated rate) when the water content was about 0.2g/g of reaction mixture, and for phosphoglucose isomerase, around 0.15g/g of reaction mixture. With the water content raised to 0.3g/g of reaction mixture the reaction rates were only increased to 0.1-3% of the fully hydrated rate. When the combined rates for phosphoglucose isomerase and glucose 6-phosphate dehydrogenase were measured, reasonable agreement was found between the experimental data and those calculated from the individual experimentally determined rates on the assumption that diffusion was not further limiting. A method was devised for measuring the diffusion coefficients of low-molecular-weight substances in solutions having low water contents. The diffusion coefficients of riboflavin in sorbitol solution decreased by about 100-fold when the water content of the latter was reduced from 3 to 0.25g/g of sorbitol. It is concluded that to detect enzyme activity a certain minimal amount of water is required and that above this minimum the rate is still restricted by diffusion limitation. The relevance of the results to the physical state of water in reaction mixtures and to metabolism in seeds and spores in xeric conditions is discussed."}
{"id": "PMID:475755", "title": "Binding of ethidium bromide and quinacrine hydrochloride to nucleic acids and reconstituted nucleohistones.", "content": "Studies of binding of ethidium bromide and quinacrine hydrochloride to native DNA at low ionic strength indicate that for both compounds the binding is selective, with about one binding site for about four nucleotides. Annealing of unfractionated histones to DNA by a salt-gradient dialysis method slightly decreases the binding of the dyes to DNA. Similar observations made with reconstituted preparations by using individual histone fractions reveal that the arginine-rich histones (histones H3 and H4) are most effective in decreasing the binding. The binding studies with ethidium bromide at high ionic strength and with denatured DNA show that strong dye binding to DNA is strongly dependent on the ionic strength and on the secondary structure of DNA. The histones are not effective in decreasing the dye binding under conditions of high ionic strength. The results are consistent with the observations [Oliver & Chalkley (1974) Biochemistry13, 5093-5098; Axel, Melchoir, Sollner-Web & Felsenfield (1974) Proc. Natl. Acad. Sci. U.S.A.71, 4101-4105] that histones form some kind of surface structures on DNA through non-specific interactions and [Kornberg & Thomas (1974) Science184, 865-868; Kornberg (1974) Science184, 868-871; D'Anna & Isenberg (1974) Biochemistry13, 4992-4997; Vandegrift, Serra, Marve & Wagner (1974) Biochemistry13, 5087-5092] that the tendency of arginine-rich histones to aggregate may be an important factor in determining the structure of chromatin.", "contents": "Binding of ethidium bromide and quinacrine hydrochloride to nucleic acids and reconstituted nucleohistones. Studies of binding of ethidium bromide and quinacrine hydrochloride to native DNA at low ionic strength indicate that for both compounds the binding is selective, with about one binding site for about four nucleotides. Annealing of unfractionated histones to DNA by a salt-gradient dialysis method slightly decreases the binding of the dyes to DNA. Similar observations made with reconstituted preparations by using individual histone fractions reveal that the arginine-rich histones (histones H3 and H4) are most effective in decreasing the binding. The binding studies with ethidium bromide at high ionic strength and with denatured DNA show that strong dye binding to DNA is strongly dependent on the ionic strength and on the secondary structure of DNA. The histones are not effective in decreasing the dye binding under conditions of high ionic strength. The results are consistent with the observations [Oliver & Chalkley (1974) Biochemistry13, 5093-5098; Axel, Melchoir, Sollner-Web & Felsenfield (1974) Proc. Natl. Acad. Sci. U.S.A.71, 4101-4105] that histones form some kind of surface structures on DNA through non-specific interactions and [Kornberg & Thomas (1974) Science184, 865-868; Kornberg (1974) Science184, 868-871; D'Anna & Isenberg (1974) Biochemistry13, 4992-4997; Vandegrift, Serra, Marve & Wagner (1974) Biochemistry13, 5087-5092] that the tendency of arginine-rich histones to aggregate may be an important factor in determining the structure of chromatin."}
{"id": "PMID:475754", "title": "Neutral glycosphingolipids and gangliosides of human lung and lung tumours.", "content": "In order to help determine whether alterations of the profiles of glycosphingolipids occur consistently in human tumours, the neutral glycosphingolipids and gangliosides of nine lung tumours (one adenocarcinoma, four squamous cell, two mixed adeno-squamous cell, one large cell and one oat-cell carcinomata) were analysed. The control tissue consisted of adjacent lung; it contained neutral glycosphingolipids corresponding in properties to glucosyl-, lactosyl-, globotriaosyl- and globotetraosyl-ceramides. All of the tumours also contained these four neutral glycosphingolipids. However, in addition, five of the tumours (two of the squamous, the large cell and the two mixed adeno-squamous cell carcinomata) contained neutral glycosphingolipids corresponding in properties to lactotriaosyl- and neolactotetraosyl-ceramides; these same tumours also exhibited higher amounts of lactosylceramide than the other tumours analysed. Both of the two former neutral glycosphingolipids and very substantial amounts of the latter neutral glycosphingolipid were detected in pneumonic lung and in polymorphonuclear leucocytes; it thus appears possible that these particular compounds were derived from these latter cells rather than from the tumour cells. The ganglioside patterns of the tumours were almost equivalent in complexity to that exhibited by the control lung tissue. This study shows that the profiles of two major classes of glycosphingolipids (neutral glycosphingolipids and gangliosides) occurring in lung tumours are almost as complex as those of the parent tissue, a finding in contrast with the notably simplified patterns of these lipids found in many cancer cells grown in vitro. It also suggests that when lactotriaosyl- and neolactotetraosyl-ceramides and high amounts of lactosylceramide are detected in human tumours, the possibility must be considered that these compounds are derived from polymorphonuclear leucocytes.", "contents": "Neutral glycosphingolipids and gangliosides of human lung and lung tumours. In order to help determine whether alterations of the profiles of glycosphingolipids occur consistently in human tumours, the neutral glycosphingolipids and gangliosides of nine lung tumours (one adenocarcinoma, four squamous cell, two mixed adeno-squamous cell, one large cell and one oat-cell carcinomata) were analysed. The control tissue consisted of adjacent lung; it contained neutral glycosphingolipids corresponding in properties to glucosyl-, lactosyl-, globotriaosyl- and globotetraosyl-ceramides. All of the tumours also contained these four neutral glycosphingolipids. However, in addition, five of the tumours (two of the squamous, the large cell and the two mixed adeno-squamous cell carcinomata) contained neutral glycosphingolipids corresponding in properties to lactotriaosyl- and neolactotetraosyl-ceramides; these same tumours also exhibited higher amounts of lactosylceramide than the other tumours analysed. Both of the two former neutral glycosphingolipids and very substantial amounts of the latter neutral glycosphingolipid were detected in pneumonic lung and in polymorphonuclear leucocytes; it thus appears possible that these particular compounds were derived from these latter cells rather than from the tumour cells. The ganglioside patterns of the tumours were almost equivalent in complexity to that exhibited by the control lung tissue. This study shows that the profiles of two major classes of glycosphingolipids (neutral glycosphingolipids and gangliosides) occurring in lung tumours are almost as complex as those of the parent tissue, a finding in contrast with the notably simplified patterns of these lipids found in many cancer cells grown in vitro. It also suggests that when lactotriaosyl- and neolactotetraosyl-ceramides and high amounts of lactosylceramide are detected in human tumours, the possibility must be considered that these compounds are derived from polymorphonuclear leucocytes."}
{"id": "PMID:475756", "title": "Interferon effect on ribosomal ribonucleic acid related to chromosome 21 ploidy.", "content": "Antiviral and cell-growth-inhibitory activities of human interferon were shown to be related to the activity of a gene or genes present on chromosome 21. The 18s rRNA is vital to cell growth; it is capable of a viral-mRNA-recognition function and it is coded for by genes a portion of which are present on chromosome-21. A previously reported ability of human interferon to affect rRNA metabolism is characterized by a decrease in the sucrose-gradient-peak ratio of radiolabelled 28S to 18S rRNA in extracts from the cytoplasm of interferon-treated human fibroblasts. In the present report, interferon dose-response curves are presented demonstrating a direct relationship between a decrease in this ratio and interferon concentrations in the media. By using this virus-independent cytoplasmic rRNA assay, eight human fibroblast lines, differing in chromosome 21 ploidy, were tested for sensitivity to human interferon. Two monosomy-21, two euploid-21 and four trisomy-21 cell lines were tested. The monosomy-21 cell populations were significantly less sensitive to interferon than the other six cell types tested. Of the cell lines tested, the most sensitive, by a wide margin, was a trisomy-21 line. Trisomy-21 cell monolayer sensitivity, however, varied widely within the range from normal to supersensitive. These observations suggest that interferon's ability to affect rRNA metabolism is related to the activity of a gene or genes present on chromosome 21.", "contents": "Interferon effect on ribosomal ribonucleic acid related to chromosome 21 ploidy. Antiviral and cell-growth-inhibitory activities of human interferon were shown to be related to the activity of a gene or genes present on chromosome 21. The 18s rRNA is vital to cell growth; it is capable of a viral-mRNA-recognition function and it is coded for by genes a portion of which are present on chromosome-21. A previously reported ability of human interferon to affect rRNA metabolism is characterized by a decrease in the sucrose-gradient-peak ratio of radiolabelled 28S to 18S rRNA in extracts from the cytoplasm of interferon-treated human fibroblasts. In the present report, interferon dose-response curves are presented demonstrating a direct relationship between a decrease in this ratio and interferon concentrations in the media. By using this virus-independent cytoplasmic rRNA assay, eight human fibroblast lines, differing in chromosome 21 ploidy, were tested for sensitivity to human interferon. Two monosomy-21, two euploid-21 and four trisomy-21 cell lines were tested. The monosomy-21 cell populations were significantly less sensitive to interferon than the other six cell types tested. Of the cell lines tested, the most sensitive, by a wide margin, was a trisomy-21 line. Trisomy-21 cell monolayer sensitivity, however, varied widely within the range from normal to supersensitive. These observations suggest that interferon's ability to affect rRNA metabolism is related to the activity of a gene or genes present on chromosome 21."}
{"id": "PMID:475757", "title": "Changes in intermediate haemoglobins during methaemoglobin reduction by NADPH-flavin reductase.", "content": "The changes in intermediate haemoglobins produced during methaemoglobin reduction by NADPH-flavin reductase were analysed by an isoelectric-focusing method. The alpha 3+ beta 2+ and alpha 2+ beta 3+ valency hybrids were observed as intermediate haemoglobins and changed consecutively with time during the reaction. On the basis of the analyses, the course of methaemoglobin reduction was found to involve two different pathways: (1) methaemoglobin kappa+1 leads to alpha 3+ beta 2+ kappa+2 leads to oxyhaemoglobin; (2) methaemoglobin kappa+3 leads to alpha 2+ beta 3+ kappa+4 leads to oxyhaemoglobin. The reaction rate constants of each phase (kappa+1--kappa+4) were also estimated. The addition of inositol hexaphosphate to the reaction mixture did not affect the overall reaction. The mechanism of methaemoglobin reduction by NADPH-flavin reductase is discussed on the basis of these results.", "contents": "Changes in intermediate haemoglobins during methaemoglobin reduction by NADPH-flavin reductase. The changes in intermediate haemoglobins produced during methaemoglobin reduction by NADPH-flavin reductase were analysed by an isoelectric-focusing method. The alpha 3+ beta 2+ and alpha 2+ beta 3+ valency hybrids were observed as intermediate haemoglobins and changed consecutively with time during the reaction. On the basis of the analyses, the course of methaemoglobin reduction was found to involve two different pathways: (1) methaemoglobin kappa+1 leads to alpha 3+ beta 2+ kappa+2 leads to oxyhaemoglobin; (2) methaemoglobin kappa+3 leads to alpha 2+ beta 3+ kappa+4 leads to oxyhaemoglobin. The reaction rate constants of each phase (kappa+1--kappa+4) were also estimated. The addition of inositol hexaphosphate to the reaction mixture did not affect the overall reaction. The mechanism of methaemoglobin reduction by NADPH-flavin reductase is discussed on the basis of these results."}
{"id": "PMID:475758", "title": "Effects of modification of the tyrosine residues of bacteriorhodopsin with tetranitromethane.", "content": "Treatment of the purple membrane of Halobacterium halobium with tetranitromethane led to modification of tyrosine residues. Modification of more than 3-4 tyrosine residues per bacteriorhodopsin monomer caused a decrease in the light-induced proton-pumping ability of purple membrane in synthetic lipid vesicles, loss of the sharp X-ray-diffraction patterns characteristic of the crystal lattice, loss of the absorbance maximum at 560 nm, and change in the buoyant density of the membrane. No modification of lipid was detected. These changes were interpreted as a gradual denaturation of the protein component such that when 8-9 tyrosine residues are modified, no proton pumping is observed. Modification of less than 3-4 tyrosine residues with tetranitromethane caused an increse in light-induced proton pumping. It was possible to generate partly modified purple membrane which had completely lost the property of diffracting X-rays into the sharp pattern observed with native purple membrane, but which still retained the ability to pump protons in a vectorial manner. Retention of crystal lattice is not essential for proton pumping.", "contents": "Effects of modification of the tyrosine residues of bacteriorhodopsin with tetranitromethane. Treatment of the purple membrane of Halobacterium halobium with tetranitromethane led to modification of tyrosine residues. Modification of more than 3-4 tyrosine residues per bacteriorhodopsin monomer caused a decrease in the light-induced proton-pumping ability of purple membrane in synthetic lipid vesicles, loss of the sharp X-ray-diffraction patterns characteristic of the crystal lattice, loss of the absorbance maximum at 560 nm, and change in the buoyant density of the membrane. No modification of lipid was detected. These changes were interpreted as a gradual denaturation of the protein component such that when 8-9 tyrosine residues are modified, no proton pumping is observed. Modification of less than 3-4 tyrosine residues with tetranitromethane caused an increse in light-induced proton pumping. It was possible to generate partly modified purple membrane which had completely lost the property of diffracting X-rays into the sharp pattern observed with native purple membrane, but which still retained the ability to pump protons in a vectorial manner. Retention of crystal lattice is not essential for proton pumping."}
{"id": "PMID:475759", "title": "The isolation, characterization and partial sequence of a peptide rich in glutamic acid and aspartic acid (HGA-2 peptide) from calf thymus non-histone chromosomal protein HMG 2. Comparison with a similar peptide (HGA-1 peptide) from calf thymus non-histone chromosomal protein HMG 1.", "content": "A 41-residue peptide (HGA-2) containing a continuous sequence of 35 glutamic and aspartic residues was isolated from non-histone chromosomal protein HMG 2. This highly acidic peptide is compared with a similar peptide (HGA-1) isolated from non-histone chromosomal protein HMG 1.", "contents": "The isolation, characterization and partial sequence of a peptide rich in glutamic acid and aspartic acid (HGA-2 peptide) from calf thymus non-histone chromosomal protein HMG 2. Comparison with a similar peptide (HGA-1 peptide) from calf thymus non-histone chromosomal protein HMG 1. A 41-residue peptide (HGA-2) containing a continuous sequence of 35 glutamic and aspartic residues was isolated from non-histone chromosomal protein HMG 2. This highly acidic peptide is compared with a similar peptide (HGA-1) isolated from non-histone chromosomal protein HMG 1."}
{"id": "PMID:475760", "title": "Biosynthesis of a 7-alpha-methoxycephalosporin. Incorporation of molecular oxygen.", "content": "A 7-alpha-methoxycephalosporin containing a carbamoyloxymethyl substituent at C-3 (cephamycin C) has been isolated from the extracellular fluid of an aqueous suspension of Streptomyces clavuligerus shaken in the presence of 18O2. The cephalosporin has been converted into its N-acetyl dimethyl ester and the distribution of 18O in the latter determined by chemical-ionization mass spectrometry. The results indicate that the oxygen atom of the methoxy group, as well as that linked to the exocyclic methylene group at C-3, is derived from molecular O2.", "contents": "Biosynthesis of a 7-alpha-methoxycephalosporin. Incorporation of molecular oxygen. A 7-alpha-methoxycephalosporin containing a carbamoyloxymethyl substituent at C-3 (cephamycin C) has been isolated from the extracellular fluid of an aqueous suspension of Streptomyces clavuligerus shaken in the presence of 18O2. The cephalosporin has been converted into its N-acetyl dimethyl ester and the distribution of 18O in the latter determined by chemical-ionization mass spectrometry. The results indicate that the oxygen atom of the methoxy group, as well as that linked to the exocyclic methylene group at C-3, is derived from molecular O2."}
{"id": "PMID:475761", "title": "Sequential degradation of a chondroitin sulphate trisaccharide by lysosomal enzymes from embryonic-chick epiphysial cartilage.", "content": "The disulphated trisaccharide D-N-acetylgalactosamine sulphate-beta-D-glucuronic acid-beta-D-N-acetylgalactosamine sulphate prepared from 35S- or 14C-labelled chondroitin sulphate was incubated with a preparation of lysosomal enzymes from embryonic-chick epiphysial cartilage. Degradation was demonstrated by analysis of the reaction products. By use of the appropriate intermediate products as substrates, in conjunction with specific enzyme inhibitors, it was shown that the degradation proceeded sequentially from the non-reducing end. It was initiated by sulphatase (preferentially hydrolysing sulphate ester groups at the 6-position), followed by beta-N-acetylgalactosaminidase and beta-glucuronidase, converting the substrate into monosaccharides and inorganic sulphate. The latter enzyme preferentially attacked disaccharides carrying their sulphate ester group at C-4 of the hexosamine residue. Generation of chondroitin sulphate oligosaccharides may occur by the action of an endoglycosidase, previously demonstrated in embryonic-chick cartilage. Endo- and exo-enzymes may thus form a functional unit in lysosomal degradation of chondroitin sulphate.", "contents": "Sequential degradation of a chondroitin sulphate trisaccharide by lysosomal enzymes from embryonic-chick epiphysial cartilage. The disulphated trisaccharide D-N-acetylgalactosamine sulphate-beta-D-glucuronic acid-beta-D-N-acetylgalactosamine sulphate prepared from 35S- or 14C-labelled chondroitin sulphate was incubated with a preparation of lysosomal enzymes from embryonic-chick epiphysial cartilage. Degradation was demonstrated by analysis of the reaction products. By use of the appropriate intermediate products as substrates, in conjunction with specific enzyme inhibitors, it was shown that the degradation proceeded sequentially from the non-reducing end. It was initiated by sulphatase (preferentially hydrolysing sulphate ester groups at the 6-position), followed by beta-N-acetylgalactosaminidase and beta-glucuronidase, converting the substrate into monosaccharides and inorganic sulphate. The latter enzyme preferentially attacked disaccharides carrying their sulphate ester group at C-4 of the hexosamine residue. Generation of chondroitin sulphate oligosaccharides may occur by the action of an endoglycosidase, previously demonstrated in embryonic-chick cartilage. Endo- and exo-enzymes may thus form a functional unit in lysosomal degradation of chondroitin sulphate."}
{"id": "PMID:475762", "title": "C1 inhibitor-dependent dissociation of human complement component C1 bound to immune complexes.", "content": "The interaction of C1 inhibitor with complement component C1 bound to immune complexes was examined by using 125I-labelled C1 subcomponents. The inhibitor binds rapidly to subcomponent C1s, and more slowly to subcomponent C1r. Formation of the C1r-C1 inhibitor complex causes rapid dissociation of subcomponents C1r and C1s from the antibody-antigen-component C1 aggregate. The rate and extent of this release are proportional to C1 Inhibitor concentration and are also dependent on ionic strength. Results obtained with purified C1 Inhibitor, plasma or serum as source of C1 Inhibitor are all closely comparable. Only slight dissociation of subcomponent C1q is observed under the same range of conditions. The implications of the release phenomenon are discussed in relation to the structure of component C1 and the possibility of differential turnover of C1 subcomponents.", "contents": "C1 inhibitor-dependent dissociation of human complement component C1 bound to immune complexes. The interaction of C1 inhibitor with complement component C1 bound to immune complexes was examined by using 125I-labelled C1 subcomponents. The inhibitor binds rapidly to subcomponent C1s, and more slowly to subcomponent C1r. Formation of the C1r-C1 inhibitor complex causes rapid dissociation of subcomponents C1r and C1s from the antibody-antigen-component C1 aggregate. The rate and extent of this release are proportional to C1 Inhibitor concentration and are also dependent on ionic strength. Results obtained with purified C1 Inhibitor, plasma or serum as source of C1 Inhibitor are all closely comparable. Only slight dissociation of subcomponent C1q is observed under the same range of conditions. The implications of the release phenomenon are discussed in relation to the structure of component C1 and the possibility of differential turnover of C1 subcomponents."}
{"id": "PMID:475763", "title": "The formation and distribution of bilirubin monoglucuronide and diglucuronide in rat liver slices.", "content": "1. Bilirubin conjugation in rat liver slices was reassessed by using analysis of ethyl anthranilate azopigments to estimate separately the formation of bilirubin mono- and di-glucuronides. 2. Conjugation in slices resembles the situation in vivo more closely than does microsomal conjugation, in that diglucuronide is formed in appreciable quantity. 3. Both bilirubin mono- and di-glucuronides were present in slices in approximately equal amounts, but the monoglucuronide was the major product found in the incubation medium. 4. These results are discussed in relation to recent theories on the relationship between bilirubin mono- and di-glucuronide formation in vivo.", "contents": "The formation and distribution of bilirubin monoglucuronide and diglucuronide in rat liver slices. 1. Bilirubin conjugation in rat liver slices was reassessed by using analysis of ethyl anthranilate azopigments to estimate separately the formation of bilirubin mono- and di-glucuronides. 2. Conjugation in slices resembles the situation in vivo more closely than does microsomal conjugation, in that diglucuronide is formed in appreciable quantity. 3. Both bilirubin mono- and di-glucuronides were present in slices in approximately equal amounts, but the monoglucuronide was the major product found in the incubation medium. 4. These results are discussed in relation to recent theories on the relationship between bilirubin mono- and di-glucuronide formation in vivo."}
{"id": "PMID:475764", "title": "The atypical velocity response by  pyruvate carboxylase to increasing concentrations of acetyl-coenzyme A.", "content": "An investigation was made of the interaction of pyruvate carboxylase with its allosteric effector, acetyl-CoA, and the velocity profile of the deacylation of acetyl-CoA as a function of acetyl-CoA concentration indicated that this ligand does not bind to this enzyme in a positive homotropic co-operative manner. An examination was therefore made of the factors that contribute to the sigmoidicity of the rate curves obtained for pyruvate carboxylation with various concentrations of acetyl-CoA. Hill coefficients for acetyl-CoA obtained with both sheep and chicken liver pyruvate carboxylases were found to be dependent on the fixed pyruvate concentration used in the assay solution. Thus, by varying the acetyl-CoA concentration, the degree of saturation of the enzyme by pyruvate was also changed. A further consequence of non-saturating concentrations of pyruvate was that the non-productive hydrolysis of the enzyme- carboxybiotin complex increased, resulting in an under-estimate of the reaction velocity measured by oxaloacetate formation. Another factor contributing to the sigmoidicity is that, at non-saturating concentrations of acetyl-CoA, the enzyme undergoes inactivation upon dilution to low protein concentrations, again resulting in an under-estimate of the reaction velocity. Under conditions where none of the above factors was operating and the only effect of varying acetyl-CoA concentrations was to alter the proportion of the enzyme catalysing the carboxylation reaction at acetyl-CoA-dependent and -independent rates, the sigmoidicity of the acetyl-CoA velocity profile was completely eliminated.", "contents": "The atypical velocity response by  pyruvate carboxylase to increasing concentrations of acetyl-coenzyme A. An investigation was made of the interaction of pyruvate carboxylase with its allosteric effector, acetyl-CoA, and the velocity profile of the deacylation of acetyl-CoA as a function of acetyl-CoA concentration indicated that this ligand does not bind to this enzyme in a positive homotropic co-operative manner. An examination was therefore made of the factors that contribute to the sigmoidicity of the rate curves obtained for pyruvate carboxylation with various concentrations of acetyl-CoA. Hill coefficients for acetyl-CoA obtained with both sheep and chicken liver pyruvate carboxylases were found to be dependent on the fixed pyruvate concentration used in the assay solution. Thus, by varying the acetyl-CoA concentration, the degree of saturation of the enzyme by pyruvate was also changed. A further consequence of non-saturating concentrations of pyruvate was that the non-productive hydrolysis of the enzyme- carboxybiotin complex increased, resulting in an under-estimate of the reaction velocity measured by oxaloacetate formation. Another factor contributing to the sigmoidicity is that, at non-saturating concentrations of acetyl-CoA, the enzyme undergoes inactivation upon dilution to low protein concentrations, again resulting in an under-estimate of the reaction velocity. Under conditions where none of the above factors was operating and the only effect of varying acetyl-CoA concentrations was to alter the proportion of the enzyme catalysing the carboxylation reaction at acetyl-CoA-dependent and -independent rates, the sigmoidicity of the acetyl-CoA velocity profile was completely eliminated."}
{"id": "PMID:475765", "title": "Analysis of the ratio of alpha- to beta-globin and globin messenger ribonucleic acid content of fractionated rabbit erythroid bone-marrow cells.", "content": "The ratio of alpha- to beta-globin mRNA was measured by hybridization of a constant amount of highly purified alpha- or beta-globin cDNA (complementary DNA) with increasing amounts of RNA in the range up to 20% cDNA hybridization, where an essentially linear reaction is obtained. Statistical analysis indicates that the ratio of alpha- to beta-globin can be measured within a maximal error of +/- 0.3 and in most cases is better than +/- 0.15. Under these conditions there is no significant deviation from the ratio of 1.3 in the alpha- to beta-globin mRNA ratio of RNA isolated from erythroid cells rich in pronormoblasts through to reticulocytes. If the ratio of alpha- to beta-globin mRNA exceeded 1.7 or was less than 0.9 in pronormoblasts, it would be detected in these experiments. The overall globin mRNA content increases to a maximal value in the fractions rich in basophilic normoblasts of 30,000--50,000 molecules/cell. However, the accuracy of these determinations is not as great as for the ratio determinations, and no significant deviations were seen except in the cells rich in pronormoblasts, which contained less globin mRNA than the later stages.", "contents": "Analysis of the ratio of alpha- to beta-globin and globin messenger ribonucleic acid content of fractionated rabbit erythroid bone-marrow cells. The ratio of alpha- to beta-globin mRNA was measured by hybridization of a constant amount of highly purified alpha- or beta-globin cDNA (complementary DNA) with increasing amounts of RNA in the range up to 20% cDNA hybridization, where an essentially linear reaction is obtained. Statistical analysis indicates that the ratio of alpha- to beta-globin can be measured within a maximal error of +/- 0.3 and in most cases is better than +/- 0.15. Under these conditions there is no significant deviation from the ratio of 1.3 in the alpha- to beta-globin mRNA ratio of RNA isolated from erythroid cells rich in pronormoblasts through to reticulocytes. If the ratio of alpha- to beta-globin mRNA exceeded 1.7 or was less than 0.9 in pronormoblasts, it would be detected in these experiments. The overall globin mRNA content increases to a maximal value in the fractions rich in basophilic normoblasts of 30,000--50,000 molecules/cell. However, the accuracy of these determinations is not as great as for the ratio determinations, and no significant deviations were seen except in the cells rich in pronormoblasts, which contained less globin mRNA than the later stages."}
{"id": "PMID:475766", "title": "Proteoglycans of the intervertebral disc. Homology of structure with laryngeal proteoglycans.", "content": "The structure of the proteoglycans from normal pig nucleus pulposus and relatively normal human annulus fibrosus and nucleus pulposus was investigated in detail and the results were compared with the current structural model of proteoglycans of hyaline cartilage. Like proteoglycans of cartilage, those of intervertebral disc contain keratan sulphate and chondroitin sulphate attached to a protein core; they are able to aggregate to hyaluronic acid; the protein core likewise has three regions, one lacking glycosaminoglycans, another rich in keratan sulphate and a third region rich in chondroitin sulphate. However, disc proteoglycans contain more keratan sulphate and protein and less chondroitin sulphate and are also considerably smaller than cartilage proteoglycans. In proteoglycans of human discs, these differences appeared to be due principally to a shorter region of the core protein bearing the chondroitin sulphate chains, whereas in proteoglycans of pig discs their smaller size and relatively low uronic acid content were due to shorter chondroitin sulphate chains. There were subtle differences between proteoglycans from the nucleus and annulus of human discs. In the latter a higher proportion of proteoglycans was capable of binding to hyaluronate.", "contents": "Proteoglycans of the intervertebral disc. Homology of structure with laryngeal proteoglycans. The structure of the proteoglycans from normal pig nucleus pulposus and relatively normal human annulus fibrosus and nucleus pulposus was investigated in detail and the results were compared with the current structural model of proteoglycans of hyaline cartilage. Like proteoglycans of cartilage, those of intervertebral disc contain keratan sulphate and chondroitin sulphate attached to a protein core; they are able to aggregate to hyaluronic acid; the protein core likewise has three regions, one lacking glycosaminoglycans, another rich in keratan sulphate and a third region rich in chondroitin sulphate. However, disc proteoglycans contain more keratan sulphate and protein and less chondroitin sulphate and are also considerably smaller than cartilage proteoglycans. In proteoglycans of human discs, these differences appeared to be due principally to a shorter region of the core protein bearing the chondroitin sulphate chains, whereas in proteoglycans of pig discs their smaller size and relatively low uronic acid content were due to shorter chondroitin sulphate chains. There were subtle differences between proteoglycans from the nucleus and annulus of human discs. In the latter a higher proportion of proteoglycans was capable of binding to hyaluronate."}
{"id": "PMID:475767", "title": "Proteoglycans of the intervertebral disc. Absence of degradation during the isolation of proteoglycans from the intervertebral disc.", "content": "Proteoglycans extracted with 4M-guanidinium chloride from pig intervetebral discs, and purified by equilibrium density-gradient centrifugation in CsCl, were of smaller hydrodynamic size than those extracted and purified in the same way from the laryngeal cartilage of the same animal. Whether this difference in size arose from degradation during the extraction and purification of the proteoglycans of the disc was investigated. Purified proteoglycans labelled either in the chondroitin sulphate chains or in the core protein were obtained from laryngeal cartilage by short-term organ culture. These labelled proteoglycans were added at the beginning of the extraction of the disc proteoglycans, and labelled cartilage and unlabelled disc proteoglycans were isolated and purified together. There was no appreciable loss of radioactivity after density-gradient centrifugation nor decrease in hydrodynamic size of the labelled cartilage proteoglycans on chromatography on Sepharose 2B, when these were present during the extraction of disc proteoglycans. It is concluded that disc proteoglycans are intrinsically of smaller size than cartilage proteoglycans and this difference in size does not arise from degradation during the extraction.", "contents": "Proteoglycans of the intervertebral disc. Absence of degradation during the isolation of proteoglycans from the intervertebral disc. Proteoglycans extracted with 4M-guanidinium chloride from pig intervetebral discs, and purified by equilibrium density-gradient centrifugation in CsCl, were of smaller hydrodynamic size than those extracted and purified in the same way from the laryngeal cartilage of the same animal. Whether this difference in size arose from degradation during the extraction and purification of the proteoglycans of the disc was investigated. Purified proteoglycans labelled either in the chondroitin sulphate chains or in the core protein were obtained from laryngeal cartilage by short-term organ culture. These labelled proteoglycans were added at the beginning of the extraction of the disc proteoglycans, and labelled cartilage and unlabelled disc proteoglycans were isolated and purified together. There was no appreciable loss of radioactivity after density-gradient centrifugation nor decrease in hydrodynamic size of the labelled cartilage proteoglycans on chromatography on Sepharose 2B, when these were present during the extraction of disc proteoglycans. It is concluded that disc proteoglycans are intrinsically of smaller size than cartilage proteoglycans and this difference in size does not arise from degradation during the extraction."}
{"id": "PMID:475768", "title": "The kinetics of rat liver and heart mitochondrial beta-hydroxybutyrate dehydrogenase.", "content": "The kinetic mechanisms of the beta-hydroxybutyrate dehydrogenase from rat heart and liver mitochondria were investigated. Both enzymes, show an Ordered Bi Bi mechanism and there are no major differences in the kinetic constants. In both cases, the solubilized enzyme, re-activated with phosphatidylcholine, shows kinetic properties very similar to those of the enzyme bound to the mitochondrial membrane.", "contents": "The kinetics of rat liver and heart mitochondrial beta-hydroxybutyrate dehydrogenase. The kinetic mechanisms of the beta-hydroxybutyrate dehydrogenase from rat heart and liver mitochondria were investigated. Both enzymes, show an Ordered Bi Bi mechanism and there are no major differences in the kinetic constants. In both cases, the solubilized enzyme, re-activated with phosphatidylcholine, shows kinetic properties very similar to those of the enzyme bound to the mitochondrial membrane."}
{"id": "PMID:475769", "title": "The multiple forms of alpha-D-mannosidase in human plasma.", "content": "The acidic alpha-D-mannosidase in human plasma closely resembles liver acidic alpha-D-mannosidase in its affinity for concanavalin A-Sepharose, molecular weight and resolution into multiple components on DEAE-cellulose. A combination of chromatography on concanavalin A-Sepharose and gel filtration on Sephadex G-200 and Sepharose 6B suggests that four forms of intermediate alpha-D-mannosidase, which differ either in their molecular weight of affinity for concanavalin A, exist in human plasma. A practical classification and nomenclature for the multiple forms of intermediate alpha-D-mannosidase in plasma based on molecular weight and affinity for concanavalin A is proposed. Multiple forms of intermediate alpha-D-mannosidase were also observed by ion-exchange chromatography on DEAE-cellulose, but there was not a simple correlation between these forms and those obtained with the other separation procedures. The form of intermediate alpha-D-mannosidase least abundant in plasma, approx. 7% of the activity, has very similar properties to the neutral alpha-D-mannosidase in human liver. In contrast, the other three forms of intermediate alpha-D-mannosidase, which account for over 90% of the activity, do not appear to be present in liver, except perhaps in trace amounts.", "contents": "The multiple forms of alpha-D-mannosidase in human plasma. The acidic alpha-D-mannosidase in human plasma closely resembles liver acidic alpha-D-mannosidase in its affinity for concanavalin A-Sepharose, molecular weight and resolution into multiple components on DEAE-cellulose. A combination of chromatography on concanavalin A-Sepharose and gel filtration on Sephadex G-200 and Sepharose 6B suggests that four forms of intermediate alpha-D-mannosidase, which differ either in their molecular weight of affinity for concanavalin A, exist in human plasma. A practical classification and nomenclature for the multiple forms of intermediate alpha-D-mannosidase in plasma based on molecular weight and affinity for concanavalin A is proposed. Multiple forms of intermediate alpha-D-mannosidase were also observed by ion-exchange chromatography on DEAE-cellulose, but there was not a simple correlation between these forms and those obtained with the other separation procedures. The form of intermediate alpha-D-mannosidase least abundant in plasma, approx. 7% of the activity, has very similar properties to the neutral alpha-D-mannosidase in human liver. In contrast, the other three forms of intermediate alpha-D-mannosidase, which account for over 90% of the activity, do not appear to be present in liver, except perhaps in trace amounts."}
{"id": "PMID:475770", "title": "Characterization of domains obtained from a mollusc haemocyanin by limited proteolytic digestion.", "content": "The haemocyanin from the freshwater gastropod Lymnaea stagnalis was digested with proteolytic enzymes under conditions where it existed as whole (native) molecules (mol.wt. approx. 9 X 10(6)), or as one-tenth molecules. Digestion of whole molecules yielded a fragment of mol.wt. approx. 110,000 believed to correspond to the 'collar' of the molecule, and an aggregate some 20--30 times the size of the original native molecule formed by end-to-end polymerization of the molecule after removal of the collar. Digestion of one-tenth molecules yielded a mixture of products that could be separated into three fractions by gel filtration. Analysis of these by sodium dodecylsulphate/polyacrylamide-gel electrophoresis revealed that they typically contained two or three components. The collar fragment was present as a component of the intermediate-molecular-weight fraction, and it dissociated on sodium dodecyl sulphate/polyacrylamide gels to give two bands corresponding to apparent mol.wts. 65,000 and 60,000. The c.d. spectra of the separated fractions were recorded and fitted with Gaussian curves by a computer procedure. The fractions each possessed distinct c.d. spectra, by which they could be identified: the collar-fragment c.d. and absorption spectra showed the most striking differences compared with those of the other fragments. The results were interpreted in terms of the postulated existence, within the haemocyanin molecule, of multi-domain structures, each comprising a single polypeptide chain of mol.wt. 200,000--300,000.", "contents": "Characterization of domains obtained from a mollusc haemocyanin by limited proteolytic digestion. The haemocyanin from the freshwater gastropod Lymnaea stagnalis was digested with proteolytic enzymes under conditions where it existed as whole (native) molecules (mol.wt. approx. 9 X 10(6)), or as one-tenth molecules. Digestion of whole molecules yielded a fragment of mol.wt. approx. 110,000 believed to correspond to the 'collar' of the molecule, and an aggregate some 20--30 times the size of the original native molecule formed by end-to-end polymerization of the molecule after removal of the collar. Digestion of one-tenth molecules yielded a mixture of products that could be separated into three fractions by gel filtration. Analysis of these by sodium dodecylsulphate/polyacrylamide-gel electrophoresis revealed that they typically contained two or three components. The collar fragment was present as a component of the intermediate-molecular-weight fraction, and it dissociated on sodium dodecyl sulphate/polyacrylamide gels to give two bands corresponding to apparent mol.wts. 65,000 and 60,000. The c.d. spectra of the separated fractions were recorded and fitted with Gaussian curves by a computer procedure. The fractions each possessed distinct c.d. spectra, by which they could be identified: the collar-fragment c.d. and absorption spectra showed the most striking differences compared with those of the other fragments. The results were interpreted in terms of the postulated existence, within the haemocyanin molecule, of multi-domain structures, each comprising a single polypeptide chain of mol.wt. 200,000--300,000."}
{"id": "PMID:475771", "title": "Purification and characterization of a phospholipase A2 from the venom of the coral snake, Micrurus fulvius microgalbineus (Brown and Smith).", "content": "A phospholipase A2 was purified from the Mexican coral snake Micrurus fulvius microgalbieus (Brown and Smith). Gel filtration of the soluble crude venom on Sephadex g-50 resolved five fractions, of which fraction II had 98% of the total phospholipase activity. This fraction was rechromatographed on a CM-cellulose column that resolved eight fractions, four of which had an important phospholipase activity. The first fraction (II-1) was homogeneous by polyacrylamide-gel electrophoresis and displayed a phospholipase specific activity of 920 units/mg of protein. The apparent molecular weight as determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis was approx. 14000. The amino acid analysis revealed the presence of 119 amino acid residues, with 12 half-cystines. the N-terminal sequence was shown to be Ser-Leu-Leu-Asx-Phe-Lys-Asx-Met-Ile-Glu-Ser-Thr..., which is homologous with that of phospholipases from other snake venoms.", "contents": "Purification and characterization of a phospholipase A2 from the venom of the coral snake, Micrurus fulvius microgalbineus (Brown and Smith). A phospholipase A2 was purified from the Mexican coral snake Micrurus fulvius microgalbieus (Brown and Smith). Gel filtration of the soluble crude venom on Sephadex g-50 resolved five fractions, of which fraction II had 98% of the total phospholipase activity. This fraction was rechromatographed on a CM-cellulose column that resolved eight fractions, four of which had an important phospholipase activity. The first fraction (II-1) was homogeneous by polyacrylamide-gel electrophoresis and displayed a phospholipase specific activity of 920 units/mg of protein. The apparent molecular weight as determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis was approx. 14000. The amino acid analysis revealed the presence of 119 amino acid residues, with 12 half-cystines. the N-terminal sequence was shown to be Ser-Leu-Leu-Asx-Phe-Lys-Asx-Met-Ile-Glu-Ser-Thr..., which is homologous with that of phospholipases from other snake venoms."}
{"id": "PMID:475772", "title": "Amino acid sequence of the N-terminal non-collagenous segment of dermatosparactic sheep procollagen type I.", "content": "The non-collagenous N-terminal segment of type I procollagen from dermatosparactic sheep skin was isolated in the form of the peptide Col 1 from a collagenase digest of the protein. The peptide has a blocked N-terminus, which was identified as pyrrolid-2-one-5-carboxylic acid. Appropriate overlapping fragments were prepared from reduced and alkylated peptide Col 1 by cleavage with trypsin at lysine, arginine and S-aminoethyl-cysteine residues and by cleavage with staphylococcal proteinase at glutamate residues. Amino acid sequence analysis of these fragments by Edman degradation and mass spectrometry established the whole sequence of peptide Col 1 except for a peptide junction (7--8) and a single Asx residue (44), and demonstrated that peptide Col 1 consists of 98 amino acid residues. The N-terminal portion of peptide Col 1 (86 residues) shows an irregular distribution of glycine, whereas the C-terminal portion (12 residues) possesses the triplet structure Gly-Xy and is apparently derived from the precursor-specific collagenous domain of procollagen. The central region of the peptide contains ten cysteine residues located between positions 18 and 73 and shows alternating polar and hydrophobic sequence elements. The regions adjacent to the cysteine-rich portion have a hydrophilic nature and are abundant in glutamic acid. The data are consistent with previous physicochemical and immunological evidence that distinct regions at the N- and C-termini of the non-collagenous domain possess a less rigid conformation than does the central portion of the molecule.", "contents": "Amino acid sequence of the N-terminal non-collagenous segment of dermatosparactic sheep procollagen type I. The non-collagenous N-terminal segment of type I procollagen from dermatosparactic sheep skin was isolated in the form of the peptide Col 1 from a collagenase digest of the protein. The peptide has a blocked N-terminus, which was identified as pyrrolid-2-one-5-carboxylic acid. Appropriate overlapping fragments were prepared from reduced and alkylated peptide Col 1 by cleavage with trypsin at lysine, arginine and S-aminoethyl-cysteine residues and by cleavage with staphylococcal proteinase at glutamate residues. Amino acid sequence analysis of these fragments by Edman degradation and mass spectrometry established the whole sequence of peptide Col 1 except for a peptide junction (7--8) and a single Asx residue (44), and demonstrated that peptide Col 1 consists of 98 amino acid residues. The N-terminal portion of peptide Col 1 (86 residues) shows an irregular distribution of glycine, whereas the C-terminal portion (12 residues) possesses the triplet structure Gly-Xy and is apparently derived from the precursor-specific collagenous domain of procollagen. The central region of the peptide contains ten cysteine residues located between positions 18 and 73 and shows alternating polar and hydrophobic sequence elements. The regions adjacent to the cysteine-rich portion have a hydrophilic nature and are abundant in glutamic acid. The data are consistent with previous physicochemical and immunological evidence that distinct regions at the N- and C-termini of the non-collagenous domain possess a less rigid conformation than does the central portion of the molecule."}
{"id": "PMID:475773", "title": "Evidence for an oleoyl phosphatidylcholine desaturase in microsomal preparations from cotyledons of safflower (Carthamus tinctorius) seed.", "content": "1. [14C]Oleoyl-CoA was metabolized rapidly and essentially completely by microsomal preparations from developing safflower (Carthamus tinctorius) cotyledons, and most of the [14C]oleate was incorporated into 3-sn-phosphatidylcholine. 2. In aerobic reaction mixtures containing NADH2 the [14C]oleate in 3-sn-phosphatidylcholine was converted into [14C]linoleate without any change in the specific radioactivity of the lipid. Over a 60 min incubation period the extent of conversion of [14C]oleoyl phosphatidylcholine into [14C]linoleoyl phosphatidylcholine was generally greater than 60%. The rate of desaturation of endogenous [14C]oleoyl phosphatidylcholine labelled from [14C]oleoyl-CoA was much greater that of exogenous [14C]dioleoyl phosphatidylcholine the specific radioactivity of the oleoyl moiety of the lipid remained constant, indicating that labelled and unlabelled oleate were desaturated at the same rate. On this assumption an initial rate of desaturation of about 15 nmol of oleate desaturated/min per mumol of 3-sn-phosphatidylcholine was estimated. 4. [14C]Oleate esterified at positions 1 and 2 of both endogenous and exogenous 3-sn-phosphatidylcholine was desaturated. 5. Attempts to demonstrate the presence of an oleoyl-CoA desaturase in safflower microsomal fractions by the appearance of linoleoyl-CoA in reaction mixtures were inconclusive.", "contents": "Evidence for an oleoyl phosphatidylcholine desaturase in microsomal preparations from cotyledons of safflower (Carthamus tinctorius) seed. 1. [14C]Oleoyl-CoA was metabolized rapidly and essentially completely by microsomal preparations from developing safflower (Carthamus tinctorius) cotyledons, and most of the [14C]oleate was incorporated into 3-sn-phosphatidylcholine. 2. In aerobic reaction mixtures containing NADH2 the [14C]oleate in 3-sn-phosphatidylcholine was converted into [14C]linoleate without any change in the specific radioactivity of the lipid. Over a 60 min incubation period the extent of conversion of [14C]oleoyl phosphatidylcholine into [14C]linoleoyl phosphatidylcholine was generally greater than 60%. The rate of desaturation of endogenous [14C]oleoyl phosphatidylcholine labelled from [14C]oleoyl-CoA was much greater that of exogenous [14C]dioleoyl phosphatidylcholine the specific radioactivity of the oleoyl moiety of the lipid remained constant, indicating that labelled and unlabelled oleate were desaturated at the same rate. On this assumption an initial rate of desaturation of about 15 nmol of oleate desaturated/min per mumol of 3-sn-phosphatidylcholine was estimated. 4. [14C]Oleate esterified at positions 1 and 2 of both endogenous and exogenous 3-sn-phosphatidylcholine was desaturated. 5. Attempts to demonstrate the presence of an oleoyl-CoA desaturase in safflower microsomal fractions by the appearance of linoleoyl-CoA in reaction mixtures were inconclusive."}
{"id": "PMID:475774", "title": "The reaction of menadione with haemoglobin. Mechanism and effect of superoxide dismutase.", "content": "1. Menadione was found to react with both the haem groups and the beta-93 thiol groups of haemoglobin. 2. It oxidized the haem groups of oxyhaemoglobin, giving mainly methaemoglobin and a smaller amount of haemichrome. The reaction rate was decrease in the presence of catalase and markedly accelerated in the presence of superoxide dismutase. It is proposed that the overall reaction involves the initial reversible formation of methaemoglobin and the semiquinone, and that the effect of superoxide dismutase is to prevent the reverse reaction, by removing superoxide and hene O2-. E.s.r. evidence for the information of the semi-quinone and its reactions is presented. 3. The reaction of menadione with the beta-93 thiol groups of haemoglobin appeared to be similar to that with other thiols, forming the 3-thioether derivative of menadione, but it was also accompanied by reduction of methaemoglobin. This reduction was prevented by superoxide dismutase, but appeared to be caused by the semiquinone radical, which was produced as an intermediate. 4. Reduced glutathione functioned only to a limited extent as a scavenger of the menadione semiquinone. Its main reaction was directly with menadione to form the thioether. Ascorbate was a more efficient scavenger, and accelerated the oxidation of oxyhaemoglobin by menadione. 5. The significance of these findings in relation to menadione-induced erythrocyte haemolysis is discussed.", "contents": "The reaction of menadione with haemoglobin. Mechanism and effect of superoxide dismutase. 1. Menadione was found to react with both the haem groups and the beta-93 thiol groups of haemoglobin. 2. It oxidized the haem groups of oxyhaemoglobin, giving mainly methaemoglobin and a smaller amount of haemichrome. The reaction rate was decrease in the presence of catalase and markedly accelerated in the presence of superoxide dismutase. It is proposed that the overall reaction involves the initial reversible formation of methaemoglobin and the semiquinone, and that the effect of superoxide dismutase is to prevent the reverse reaction, by removing superoxide and hene O2-. E.s.r. evidence for the information of the semi-quinone and its reactions is presented. 3. The reaction of menadione with the beta-93 thiol groups of haemoglobin appeared to be similar to that with other thiols, forming the 3-thioether derivative of menadione, but it was also accompanied by reduction of methaemoglobin. This reduction was prevented by superoxide dismutase, but appeared to be caused by the semiquinone radical, which was produced as an intermediate. 4. Reduced glutathione functioned only to a limited extent as a scavenger of the menadione semiquinone. Its main reaction was directly with menadione to form the thioether. Ascorbate was a more efficient scavenger, and accelerated the oxidation of oxyhaemoglobin by menadione. 5. The significance of these findings in relation to menadione-induced erythrocyte haemolysis is discussed."}
{"id": "PMID:475775", "title": "The determination of binding parameters when the total and free substrate concentrations are not approximately equal.", "content": "By using a standard graphical method values of Km and V may be found that are independent of the conditions and assumptions that the total substrate concentration approximates to its free concentration and that Km is much larger than the enzyme concentration. The procedure is also applicable to the determination of equilibrium binding parameters of a ligand to a macromolecule.", "contents": "The determination of binding parameters when the total and free substrate concentrations are not approximately equal. By using a standard graphical method values of Km and V may be found that are independent of the conditions and assumptions that the total substrate concentration approximates to its free concentration and that Km is much larger than the enzyme concentration. The procedure is also applicable to the determination of equilibrium binding parameters of a ligand to a macromolecule."}
{"id": "PMID:475776", "title": "The separation of sheep liver cytoplasmic and mitochondrial aldehyde dehydrogenases by isoelectric focusing, and observations on the purity of preparations of the cytoplasmic enzyme, and their sensitivity towards inhibition by disulfiram.", "content": "Preparations of sheep liver cytoplasmic aldehyde dehydrogenase obtained by published methods were found by analytical isoelectric focusing in the pH range 5--8 to contain 5--10% by weight of the mitochondrial aldehyde dehydrogenase. Under the conditions used the pI of the cytoplasmic enzyme is 6.2 and that of the mitochondrial enzyme 6.6. The mitochondrial enzyme can be removed from the preparation by selective precipitation of the cytoplasmic enzyme with (NH4)2SO4. Kinetic experiments and inhibition experiments with disulfiram show that the properties of the two sheep liver enzymes are so different that the presence of 10% mitochondrial enzyme in preparations of the cytoplasmic enzyme can introduce serious errors into results. Our results suggest that the presence of 10 microM-disulfiram in assays may completely inactivate the pure cytoplasmic enzyme. This result is in contrast with a previous report [kitson (1978) Biochem. U. 175, 83--90].", "contents": "The separation of sheep liver cytoplasmic and mitochondrial aldehyde dehydrogenases by isoelectric focusing, and observations on the purity of preparations of the cytoplasmic enzyme, and their sensitivity towards inhibition by disulfiram. Preparations of sheep liver cytoplasmic aldehyde dehydrogenase obtained by published methods were found by analytical isoelectric focusing in the pH range 5--8 to contain 5--10% by weight of the mitochondrial aldehyde dehydrogenase. Under the conditions used the pI of the cytoplasmic enzyme is 6.2 and that of the mitochondrial enzyme 6.6. The mitochondrial enzyme can be removed from the preparation by selective precipitation of the cytoplasmic enzyme with (NH4)2SO4. Kinetic experiments and inhibition experiments with disulfiram show that the properties of the two sheep liver enzymes are so different that the presence of 10% mitochondrial enzyme in preparations of the cytoplasmic enzyme can introduce serious errors into results. Our results suggest that the presence of 10 microM-disulfiram in assays may completely inactivate the pure cytoplasmic enzyme. This result is in contrast with a previous report [kitson (1978) Biochem. U. 175, 83--90]."}
{"id": "PMID:475777", "title": "Co-ordinate regulation of ethanolamine kinase and phosphoethanolamine cytidylyltransferase in the biosynthesis of phosphatidylethanolamine in rat liver.", "content": "Essential-fatty acid deficiency produces a 52% increase in the rate of phosphatidyl-ethanolamine synthesis in rat liver as calculated from results obtained in vivo [Trewhella & Collins (1973) Biochem. Biophys. Acta 296, 34--50]. This flux change was used to test the possible regulatory roles of ethanolamine kinase and of phosphoethanolamine cytidylyltransferase, which are rate-limiting enzymes of the cytidine pathway for the synthesis of phosphatidylethanolamine [Infante (1977) Biochem. J. 167, 847--849]. The results show that essential-fatty acid deficiency produces 50% and 53% increases respectively in the specific activity of these enzymes, accounting for the increased rate of phosphatidylethanolamine synthesis produced by this dietary insufficiency. This evidence leads to the conclusion that ethanolamine kinase and phosphoethanolamine cytidylyl-transferase have co-ordinated regulatory roles in the flux control of the cytidine pathway, and its sphinganine 1-phosphate lyase branch reaction, for the synthesis of phosphatidylethanolamine.", "contents": "Co-ordinate regulation of ethanolamine kinase and phosphoethanolamine cytidylyltransferase in the biosynthesis of phosphatidylethanolamine in rat liver. Essential-fatty acid deficiency produces a 52% increase in the rate of phosphatidyl-ethanolamine synthesis in rat liver as calculated from results obtained in vivo [Trewhella & Collins (1973) Biochem. Biophys. Acta 296, 34--50]. This flux change was used to test the possible regulatory roles of ethanolamine kinase and of phosphoethanolamine cytidylyltransferase, which are rate-limiting enzymes of the cytidine pathway for the synthesis of phosphatidylethanolamine [Infante (1977) Biochem. J. 167, 847--849]. The results show that essential-fatty acid deficiency produces 50% and 53% increases respectively in the specific activity of these enzymes, accounting for the increased rate of phosphatidylethanolamine synthesis produced by this dietary insufficiency. This evidence leads to the conclusion that ethanolamine kinase and phosphoethanolamine cytidylyl-transferase have co-ordinated regulatory roles in the flux control of the cytidine pathway, and its sphinganine 1-phosphate lyase branch reaction, for the synthesis of phosphatidylethanolamine."}
{"id": "PMID:475868", "title": "Early complement component depletion and mixed cryoglobulinemia in a \"healthy\" family.", "content": "A family containing two apparently healthy brothers with profound early complement component depletion (C1, C4, C2) and mixed IgG-IgM polyclonal cryoglobulins was studied. The cryoglobulins possessed rheumatoid factor activity and depleted early complement components in normal human serum. Circulating immune complexes could not be detected by utilizing standard methods. The phenomenon was not HLA-linked. This study demonstrates the familial occurrence of a chronic hypocomplementemic state associated with cryoglobulinemia in clinically normal subjects.", "contents": "Early complement component depletion and mixed cryoglobulinemia in a \"healthy\" family. A family containing two apparently healthy brothers with profound early complement component depletion (C1, C4, C2) and mixed IgG-IgM polyclonal cryoglobulins was studied. The cryoglobulins possessed rheumatoid factor activity and depleted early complement components in normal human serum. Circulating immune complexes could not be detected by utilizing standard methods. The phenomenon was not HLA-linked. This study demonstrates the familial occurrence of a chronic hypocomplementemic state associated with cryoglobulinemia in clinically normal subjects."}
{"id": "PMID:475873", "title": "Does corticosteroid therapy affect the survival of patients with systemic lupus erythematosus?", "content": "The hypothesis that corticosteroid therapy is responsible for the striking improvement in survival of patients with systemic lupus erythematosus (SLE) was investigated with two approaches: Of 250 published papers on SLE, 52 were chosen for the first analysis because they included sufficient information on diagnostic criteria and survival but were not limited to patients selected for a particular target organ. From each article percent survival by series, average duration to death, and 1 and 5 year survival curves were abstracted. Each statistic showed linear improvement in survival since the 1930s without a significant change (P greater than 0.10) in slope for the time period following the introduction of corticosteroids. The second analysis examined the effect of corticosteroid therapy on 142 patients with SLE followed at the Massachusetts General Hospital between 1922 and 1966. Although the steroid use was conservative, the patients' survival, from year of entry, was comparable to the 52 abstracted series. A prognostic index was used to stratify patients admitted in the steroid era (after 1950) for life table analysis of survival with and without steroids. Steroids had no discernible effect on overall survival in low and medium risk groups. Use of steroids was associated with improved survival among high rish patients (P less than 0.05).", "contents": "Does corticosteroid therapy affect the survival of patients with systemic lupus erythematosus? The hypothesis that corticosteroid therapy is responsible for the striking improvement in survival of patients with systemic lupus erythematosus (SLE) was investigated with two approaches: Of 250 published papers on SLE, 52 were chosen for the first analysis because they included sufficient information on diagnostic criteria and survival but were not limited to patients selected for a particular target organ. From each article percent survival by series, average duration to death, and 1 and 5 year survival curves were abstracted. Each statistic showed linear improvement in survival since the 1930s without a significant change (P greater than 0.10) in slope for the time period following the introduction of corticosteroids. The second analysis examined the effect of corticosteroid therapy on 142 patients with SLE followed at the Massachusetts General Hospital between 1922 and 1966. Although the steroid use was conservative, the patients' survival, from year of entry, was comparable to the 52 abstracted series. A prognostic index was used to stratify patients admitted in the steroid era (after 1950) for life table analysis of survival with and without steroids. Steroids had no discernible effect on overall survival in low and medium risk groups. Use of steroids was associated with improved survival among high rish patients (P less than 0.05)."}
{"id": "PMID:475874", "title": "IgG rheumatoid factor. Relationship to seropositive rheumatoid arthritis and absence in seronegative disorders.", "content": "IgG rheumatoid factor was detected in the sera of the majority of patients with seropositive rheumatoid arthritis. Values suggestive of IgG rheumatoid factor were noted in one-quarter of patients with seronegative inflammatory arthropathies. These determinations were always low and correlated with elevated IgG concentrations, suggesting nonspecific adherence of IgG rather than a true antigen-antibody reaction. In support of this conclusion, nonrheumatoid factor IgG was capable of concentration-dependent nonspecific adsorption to the solid phase. IgG, but not IgM, rheumatoid factor corresponded with disease activity in patients with seropositive rheumatoid arthritis, suggesting that IgG rheumatoid factor may be important in the pathogenesis.", "contents": "IgG rheumatoid factor. Relationship to seropositive rheumatoid arthritis and absence in seronegative disorders. IgG rheumatoid factor was detected in the sera of the majority of patients with seropositive rheumatoid arthritis. Values suggestive of IgG rheumatoid factor were noted in one-quarter of patients with seronegative inflammatory arthropathies. These determinations were always low and correlated with elevated IgG concentrations, suggesting nonspecific adherence of IgG rather than a true antigen-antibody reaction. In support of this conclusion, nonrheumatoid factor IgG was capable of concentration-dependent nonspecific adsorption to the solid phase. IgG, but not IgM, rheumatoid factor corresponded with disease activity in patients with seropositive rheumatoid arthritis, suggesting that IgG rheumatoid factor may be important in the pathogenesis."}
{"id": "PMID:475875", "title": "The rarity of Felty's syndrome in blacks.", "content": "Evidence is presented that Felty's syndrome (FS) is rare among black patients with rheumatoid arthritis (RA). All of 12 patients with FS seen at Parkland Memorial Hospital, Dallas, Texas betwen 1964 and 1978 were white. During this period 52% of patients admitted to the Parkland medical service were black and 31% of patients dischargd with a diagnosis of RA were black. The number of expected black cases of FS on the basis of the racial distribution of hospitalized patients with RA was 3.7 (P is less than 0.02 when the zero incidence in blacks was compared with the expected incidence). All 7 cases of FS observed at Charity Hospital, New Orleans, Louisiana between 1968 and 1978 were also white. During this period, 65% of patients discharged with a diagnosis of RA were black, and the number of expected black cases of FS was 4.5 (P is less than 0.001). These findings suggest a genetic basis for the development of leukopenia and splenomegaly in RA patients.", "contents": "The rarity of Felty's syndrome in blacks. Evidence is presented that Felty's syndrome (FS) is rare among black patients with rheumatoid arthritis (RA). All of 12 patients with FS seen at Parkland Memorial Hospital, Dallas, Texas betwen 1964 and 1978 were white. During this period 52% of patients admitted to the Parkland medical service were black and 31% of patients dischargd with a diagnosis of RA were black. The number of expected black cases of FS on the basis of the racial distribution of hospitalized patients with RA was 3.7 (P is less than 0.02 when the zero incidence in blacks was compared with the expected incidence). All 7 cases of FS observed at Charity Hospital, New Orleans, Louisiana between 1968 and 1978 were also white. During this period, 65% of patients discharged with a diagnosis of RA were black, and the number of expected black cases of FS was 4.5 (P is less than 0.001). These findings suggest a genetic basis for the development of leukopenia and splenomegaly in RA patients."}
{"id": "PMID:475876", "title": "Neurogenic regulation of lipid metabolism in the rabbit. A mechanism for the cholesterol-lowering effect of acupuncture.", "content": "Plasma and aortic cholesterol levels are known to fall significantly after needling at the specific point which is innervated by a branch of the deep peroneal nerve. Different types of stimulation were applied to the specific nerve, using solely needling, injection of normal saline with needling and injection of a small dose of novocain with needling at the specific point in several groups of rabbits fed a high cholesterol diet for two or three weeks. Significant decreases in plasma and aortic cholesterol were obtained in the combined acupuncture group after one or two weeks of acupuncture. When the specific point was blocked by injection of a larger dose of novocain or the deep peroneal nerve was cut, then needling at the specific point lost its hypocholesterolemic effect. It is thus suspected that stimulation of sensory receptors of the deep peroneal nerve at the specific point may be the mechanism of the cholesterol-lowering action of acupuncture.", "contents": "Neurogenic regulation of lipid metabolism in the rabbit. A mechanism for the cholesterol-lowering effect of acupuncture. Plasma and aortic cholesterol levels are known to fall significantly after needling at the specific point which is innervated by a branch of the deep peroneal nerve. Different types of stimulation were applied to the specific nerve, using solely needling, injection of normal saline with needling and injection of a small dose of novocain with needling at the specific point in several groups of rabbits fed a high cholesterol diet for two or three weeks. Significant decreases in plasma and aortic cholesterol were obtained in the combined acupuncture group after one or two weeks of acupuncture. When the specific point was blocked by injection of a larger dose of novocain or the deep peroneal nerve was cut, then needling at the specific point lost its hypocholesterolemic effect. It is thus suspected that stimulation of sensory receptors of the deep peroneal nerve at the specific point may be the mechanism of the cholesterol-lowering action of acupuncture."}
{"id": "PMID:475878", "title": "Some particular aspects of the microarchitecture of human coronary arteries.", "content": "The following light-microscopic features characterize the microarchitecture of human coronary arteries compared with other organ arteries of muscular type and similar size: (a) the rapid development of a diffuse thickened intima which in adolescents becomes the main layer of the coronary artery wall; (b) the slow post-natal increase in the number of circularly oriented rows of smooth muscle cells of the media; (c) the occurrence of numerous longitudinal muscle columns, particularly in arterial segments and branches which run from the base to the apex of the heart; (d) the complex organization of branching points, including the presence of large pads or cushions, a very thick intima and many longitudinal muscle columns, some of which exhibit a spiral course at the level of the branch mouths with the formation of muscle rings round the entrance orifices.", "contents": "Some particular aspects of the microarchitecture of human coronary arteries. The following light-microscopic features characterize the microarchitecture of human coronary arteries compared with other organ arteries of muscular type and similar size: (a) the rapid development of a diffuse thickened intima which in adolescents becomes the main layer of the coronary artery wall; (b) the slow post-natal increase in the number of circularly oriented rows of smooth muscle cells of the media; (c) the occurrence of numerous longitudinal muscle columns, particularly in arterial segments and branches which run from the base to the apex of the heart; (d) the complex organization of branching points, including the presence of large pads or cushions, a very thick intima and many longitudinal muscle columns, some of which exhibit a spiral course at the level of the branch mouths with the formation of muscle rings round the entrance orifices."}
{"id": "PMID:475879", "title": "Study of fibrous plaques occurring in the coronary arteries of children.", "content": "The pathology of the coronary arteries of children, in relation to atherosclerotic involvement, appeared as a pathology of the main emergence areas and branching points of the left coronary artery and particularly of the anterior descending artery. The first atherosclerotic lesions occurred as non-raised fibrous plaques in 2% of children 6--10 years old and in 4% of children and juveniles 11--15 years old. In the latter age group fatty streaks and gelatinous plaques were also seen in 6% of the subjects; in their appearance they lag behind fibrous plaques by 5--8 years. Indirect evidence was obtained that some branch pads or cushions might be converted into fibrous plaques. The transitional aspects included edema, histolysis (elastolysis, collagenolysis, ground-substance depletion and degenerative cell changes), followed by reorganization and homogeneization of the pre-existing heterogeneous microarchitecture; in a final stage the prevalent processes seemed to be the nodular proliferation of smooth muscle cells and the abundant neoformation of collagen fibers. In essence the results show that in atherosclerosis the coronary arteries are involved in a different sequence and histogenetic pattern than the aorta.", "contents": "Study of fibrous plaques occurring in the coronary arteries of children. The pathology of the coronary arteries of children, in relation to atherosclerotic involvement, appeared as a pathology of the main emergence areas and branching points of the left coronary artery and particularly of the anterior descending artery. The first atherosclerotic lesions occurred as non-raised fibrous plaques in 2% of children 6--10 years old and in 4% of children and juveniles 11--15 years old. In the latter age group fatty streaks and gelatinous plaques were also seen in 6% of the subjects; in their appearance they lag behind fibrous plaques by 5--8 years. Indirect evidence was obtained that some branch pads or cushions might be converted into fibrous plaques. The transitional aspects included edema, histolysis (elastolysis, collagenolysis, ground-substance depletion and degenerative cell changes), followed by reorganization and homogeneization of the pre-existing heterogeneous microarchitecture; in a final stage the prevalent processes seemed to be the nodular proliferation of smooth muscle cells and the abundant neoformation of collagen fibers. In essence the results show that in atherosclerosis the coronary arteries are involved in a different sequence and histogenetic pattern than the aorta."}
{"id": "PMID:475880", "title": "The fatty acid pattern of triglycerides and FFA in serum of spontaneously hypertensive rats (SHR).", "content": "The fatty acid patterns of serum triglycerides and FFA in SHR and in normotensive controls aged 4, 8 and 26 weeks were estimated by gas-liquid chromatography. In serum triglycerides of SHR, the percentage of linoleic acid (C18:2) was lower and the content of arachidonic acid (C20:4) higher than in age-matched control animals. A continuous increase in palmitic (C16) and linoleic acids as well as a decrease in arachidonic acid has been found with advancing age, the most striking differences existing between 4- and 8-week-old animals, i.e. before onset of arterial hypertension in SHR. In the pre-hypertensive stage, the percentage of arachidonic acid was about 3 times as high as in later stages in SHR. This gradation was, however, even more pronounced in normotensive control rats. The C18:2/C20:4-ratio of triglycerides was lower in SHR but increased with age in both groups reaching a 5--8-fold level. Similar behavior of the FFA fatty acid pattern was less marked. Alterations in levels of linoleic and arachidonic acids are of interest because of their pathogenic role as precursors of prostaglandins in the development of genetically spontaneous hypertension in rats. The results are discussed in connection with the hypotensive effect of a linoleic acid-rich diet recently reported in hypertensive rats.", "contents": "The fatty acid pattern of triglycerides and FFA in serum of spontaneously hypertensive rats (SHR). The fatty acid patterns of serum triglycerides and FFA in SHR and in normotensive controls aged 4, 8 and 26 weeks were estimated by gas-liquid chromatography. In serum triglycerides of SHR, the percentage of linoleic acid (C18:2) was lower and the content of arachidonic acid (C20:4) higher than in age-matched control animals. A continuous increase in palmitic (C16) and linoleic acids as well as a decrease in arachidonic acid has been found with advancing age, the most striking differences existing between 4- and 8-week-old animals, i.e. before onset of arterial hypertension in SHR. In the pre-hypertensive stage, the percentage of arachidonic acid was about 3 times as high as in later stages in SHR. This gradation was, however, even more pronounced in normotensive control rats. The C18:2/C20:4-ratio of triglycerides was lower in SHR but increased with age in both groups reaching a 5--8-fold level. Similar behavior of the FFA fatty acid pattern was less marked. Alterations in levels of linoleic and arachidonic acids are of interest because of their pathogenic role as precursors of prostaglandins in the development of genetically spontaneous hypertension in rats. The results are discussed in connection with the hypotensive effect of a linoleic acid-rich diet recently reported in hypertensive rats."}
{"id": "PMID:475881", "title": "Early increase of a new platelet coagulant activity in rats fed a thrombogenic diet.", "content": "A recently described platelet coagulant activity (factor X activating activity), whose pathophysiological significance is as yet unknown, was studied in rats fed a fat-rich (thrombogenic) diet for 1, 2 and 7 weeks and compared to rats fed normal laboratory chow. Whatever the duration of the special feeding period, a highly significant shortening of the clotting time, used for measuring this activity, was observed. When the platelet coagulant activity of individual \"fat-fed\"rats was quantitated by reference to that of individual control animals, we found a mean increase of 350% (n = 9) after one week and 267% (n = 3) after two weeks of dietary treatment. Partial thromboplastin time, thrombin time and soluble fibrin monomer complexes did not differ in control and treated animals. It seems that platelet coagulant activity, as measured in our test system, is one of the first laboratory parameters to be modified by fat-rich diets. These findings may be relevant to an understanding of the role of platelet coagulant activities other than platelet factor 3 in thrombotic phenomena.", "contents": "Early increase of a new platelet coagulant activity in rats fed a thrombogenic diet. A recently described platelet coagulant activity (factor X activating activity), whose pathophysiological significance is as yet unknown, was studied in rats fed a fat-rich (thrombogenic) diet for 1, 2 and 7 weeks and compared to rats fed normal laboratory chow. Whatever the duration of the special feeding period, a highly significant shortening of the clotting time, used for measuring this activity, was observed. When the platelet coagulant activity of individual \"fat-fed\"rats was quantitated by reference to that of individual control animals, we found a mean increase of 350% (n = 9) after one week and 267% (n = 3) after two weeks of dietary treatment. Partial thromboplastin time, thrombin time and soluble fibrin monomer complexes did not differ in control and treated animals. It seems that platelet coagulant activity, as measured in our test system, is one of the first laboratory parameters to be modified by fat-rich diets. These findings may be relevant to an understanding of the role of platelet coagulant activities other than platelet factor 3 in thrombotic phenomena."}
{"id": "PMID:475882", "title": "Increased growth of human fibroblasts and arterial smooth muscle cells from diabetic patients related to diabetic serum factors and cell origin.", "content": "Fibroblasts from 3 diabetic patients (DF) grew faster, resulting in higher cell counts in the stationary phase than fibroblasts from 3 age-matched healthy volunteers (NF). This difference was apparent when DF or NF were cultured in either diabetic (DS) or normal serum (NS). Diabetic serum increased growth of both DF and NF compared with normal serum. Total protein content per plate paralleled the increase of cell number per plate in relation to cell origin and serum type. DS increased growth and total protein per plate in the arterial smooth muscle cell line from a non-diabetic patient in a way similar to in DF and NF. It is concluded that increased growth of DF in vivo could result in an increased turnover of vascular cells with a shortened replicative lifespan, leading to an accumulation of basal lamina. This effect would be even further accentuated by exposure of DF to DS. Taken together with the increased protein synthesis the accelerated development of diabetic angiopathy could be the final consequence.", "contents": "Increased growth of human fibroblasts and arterial smooth muscle cells from diabetic patients related to diabetic serum factors and cell origin. Fibroblasts from 3 diabetic patients (DF) grew faster, resulting in higher cell counts in the stationary phase than fibroblasts from 3 age-matched healthy volunteers (NF). This difference was apparent when DF or NF were cultured in either diabetic (DS) or normal serum (NS). Diabetic serum increased growth of both DF and NF compared with normal serum. Total protein content per plate paralleled the increase of cell number per plate in relation to cell origin and serum type. DS increased growth and total protein per plate in the arterial smooth muscle cell line from a non-diabetic patient in a way similar to in DF and NF. It is concluded that increased growth of DF in vivo could result in an increased turnover of vascular cells with a shortened replicative lifespan, leading to an accumulation of basal lamina. This effect would be even further accentuated by exposure of DF to DS. Taken together with the increased protein synthesis the accelerated development of diabetic angiopathy could be the final consequence."}
{"id": "PMID:475883", "title": "Lipoprotein lipase activity in human adipose tissue during induced hypertriglyceridaemia.", "content": "Needle biopsies of adipose tissue and blood samples were obtained before and at short intervals after a \"bolus\" injection of 10% Intralipid. Lipoprotein lipase activities were measured in acetone--ether extracts of the tissue samples. Levels of serum triglyceride began to fall less than 5 min after the injection of the Intralipid with a half-life of 20 min. During this time interval, no significant changes were observed in the activities of lipoprotein lipase in adipose tissue. A patient with a severe hypertriglyceridaemia (Type V) underwent plasma exchange with a reduction in serum triglyceride levels from 11 to 4 mm/l. There was a parallel fall in adipose tissue lipoprotein lipase activity. We conclude that lipoprotein lipase in adipose tissue is unaltered during experimental hypertriglyceridaemia and that the activity of the enzyme in adipose tissue is probably not reduced as a secondary feature of an elevated plasma triglyceride level.", "contents": "Lipoprotein lipase activity in human adipose tissue during induced hypertriglyceridaemia. Needle biopsies of adipose tissue and blood samples were obtained before and at short intervals after a \"bolus\" injection of 10% Intralipid. Lipoprotein lipase activities were measured in acetone--ether extracts of the tissue samples. Levels of serum triglyceride began to fall less than 5 min after the injection of the Intralipid with a half-life of 20 min. During this time interval, no significant changes were observed in the activities of lipoprotein lipase in adipose tissue. A patient with a severe hypertriglyceridaemia (Type V) underwent plasma exchange with a reduction in serum triglyceride levels from 11 to 4 mm/l. There was a parallel fall in adipose tissue lipoprotein lipase activity. We conclude that lipoprotein lipase in adipose tissue is unaltered during experimental hypertriglyceridaemia and that the activity of the enzyme in adipose tissue is probably not reduced as a secondary feature of an elevated plasma triglyceride level."}
{"id": "PMID:475884", "title": "Adipose cell size in spontaneously hypertensive rats (SHR).", "content": "The size of adipose cells in spontaneously hypertensive rats (SHR) and normotensive controls has been evaluated at 4, 8 and 26 weeks of age. Age-matched groups showed significant differences only in 8-week-old rats, but this can be explained by the lower body weight of SHR. In both groups of animals fat cell size varies with body weight (r = 0.965 in SHR and r = 0.863 in normotensive rats) independent of the stage of hypertension. The regression lines are not significantly different. Thus, no evidence of enlarged adipocytes in SHR has been obtained.", "contents": "Adipose cell size in spontaneously hypertensive rats (SHR). The size of adipose cells in spontaneously hypertensive rats (SHR) and normotensive controls has been evaluated at 4, 8 and 26 weeks of age. Age-matched groups showed significant differences only in 8-week-old rats, but this can be explained by the lower body weight of SHR. In both groups of animals fat cell size varies with body weight (r = 0.965 in SHR and r = 0.863 in normotensive rats) independent of the stage of hypertension. The regression lines are not significantly different. Thus, no evidence of enlarged adipocytes in SHR has been obtained."}
{"id": "PMID:475888", "title": "Properties of flavins where the 8-methyl group is replaced by mercapto- residues.", "content": "Sulfur functions in position 8 of the flavin nucleus give rise to new modified flavin derivatives, which should prove useful as probes of the flavin binding domains of flavoproteins. Here, we report on some properties of 8-nor-8-alkylmercaptoflavins and 8-nor-8-mercaptoflavin which are readily formed by nucleophilic displacement by alkylmercaptides or sulfide, with 8-nor-8-chloroflavins as starting material. The new flavins are characterized by extensive shifts in spectral properties, with very high extinction coefficients. 8-nor-8-mercaptoriboflavin is easily and reversibly converted to its (-S-S-) dimer. Oxidation of the sulfur group by peracids forms first sulfoxides and then sulfones, in which the characteristic usual flavin spectrum is regained. A comparison of 8-SR-8-nor-flavins with 8-OR-8-nor-flavins (Ghisla, S., and Mayhew, S.G. (1976) Eur. J. Biochem 63, 373-390) indicates that in both classes of compounds, optical properties, ionization constants, and oxidation-reduction potentials follow similar patterns.", "contents": "Properties of flavins where the 8-methyl group is replaced by mercapto- residues. Sulfur functions in position 8 of the flavin nucleus give rise to new modified flavin derivatives, which should prove useful as probes of the flavin binding domains of flavoproteins. Here, we report on some properties of 8-nor-8-alkylmercaptoflavins and 8-nor-8-mercaptoflavin which are readily formed by nucleophilic displacement by alkylmercaptides or sulfide, with 8-nor-8-chloroflavins as starting material. The new flavins are characterized by extensive shifts in spectral properties, with very high extinction coefficients. 8-nor-8-mercaptoriboflavin is easily and reversibly converted to its (-S-S-) dimer. Oxidation of the sulfur group by peracids forms first sulfoxides and then sulfones, in which the characteristic usual flavin spectrum is regained. A comparison of 8-SR-8-nor-flavins with 8-OR-8-nor-flavins (Ghisla, S., and Mayhew, S.G. (1976) Eur. J. Biochem 63, 373-390) indicates that in both classes of compounds, optical properties, ionization constants, and oxidation-reduction potentials follow similar patterns."}
{"id": "PMID:475889", "title": "Chromosomal breakage in Crohn's disease: anticlastogenic effect of D-penicillamine and L-cysteine.", "content": "The incidence of chromosome breakage was found to be elevated in 42 patients with Crohn's disease. This phenomenon was much more striking in cultures set up with TCM 199 than in cultures set up with RPMI 1629 rich in L-cysteine. The drug D-penicillamine, a close analog of L-cysteine, gave an apparent therapeutic response in several patients and reduced the chromosome breakage frequency in the lymphocytes of these patients in vitro and in vivo.", "contents": "Chromosomal breakage in Crohn's disease: anticlastogenic effect of D-penicillamine and L-cysteine. The incidence of chromosome breakage was found to be elevated in 42 patients with Crohn's disease. This phenomenon was much more striking in cultures set up with TCM 199 than in cultures set up with RPMI 1629 rich in L-cysteine. The drug D-penicillamine, a close analog of L-cysteine, gave an apparent therapeutic response in several patients and reduced the chromosome breakage frequency in the lymphocytes of these patients in vitro and in vivo."}
{"id": "PMID:475890", "title": "Subclinical effects of chronic increased lead absorption--a prospective study. I. Study design and analysis of symptoms.", "content": "Seventy workers exposed to lead for at least one year and 35 control workers have been enrolled in a prospective study of possible neurologic effects of chronic lead absorption at or below the current standard of 80 microgram per 100 ml whole blood. The study design is described in detail. Initial results of analysis of lead-related symptoms from baseline studies indicate few differences between the exposed and nonexposed workers. The majority of differences were for central nervous system (CNS) symptoms and muscle or joint pain. Little correlation was found between symptom reporting and indices of lead absorption. The evidence suggests that factors other than lead absorption itself may be important in symptom reporting.", "contents": "Subclinical effects of chronic increased lead absorption--a prospective study. I. Study design and analysis of symptoms. Seventy workers exposed to lead for at least one year and 35 control workers have been enrolled in a prospective study of possible neurologic effects of chronic lead absorption at or below the current standard of 80 microgram per 100 ml whole blood. The study design is described in detail. Initial results of analysis of lead-related symptoms from baseline studies indicate few differences between the exposed and nonexposed workers. The majority of differences were for central nervous system (CNS) symptoms and muscle or joint pain. Little correlation was found between symptom reporting and indices of lead absorption. The evidence suggests that factors other than lead absorption itself may be important in symptom reporting."}
{"id": "PMID:475892", "title": "[Treatment of cardiac insufficiency in the acute stage of cardiac infarct].", "content": "The studies on Strophantin effect upon the hemodynamics in the acute stage of cardiac infarction has revealed that it elevates the pressure in pulmonary artery and reduces the cardiac output, in certain cases deteriorating the patient's state. Furanthril and Droperidol reduce the cardiac output but at the same time diminish the pressure in the pulmonary artery and reduce the pulmonary resistance, thus having a favourable effect on the patient's state. Proceeding from the results obtained, a treatment scheme, including Furanthril, Droperidol and Strophantin, is recommended for the cardiac insufficiency treatment in the acute stage of cardiac infarction depending on the disease onset and the degree of cardiac insufficiency manifested.", "contents": "[Treatment of cardiac insufficiency in the acute stage of cardiac infarct]. The studies on Strophantin effect upon the hemodynamics in the acute stage of cardiac infarction has revealed that it elevates the pressure in pulmonary artery and reduces the cardiac output, in certain cases deteriorating the patient's state. Furanthril and Droperidol reduce the cardiac output but at the same time diminish the pressure in the pulmonary artery and reduce the pulmonary resistance, thus having a favourable effect on the patient's state. Proceeding from the results obtained, a treatment scheme, including Furanthril, Droperidol and Strophantin, is recommended for the cardiac insufficiency treatment in the acute stage of cardiac infarction depending on the disease onset and the degree of cardiac insufficiency manifested."}
{"id": "PMID:475893", "title": "[Dynamic changes in the indices of myocardial infarct patients at the polyclinical stage of rehabilitation].", "content": "Twenty five patients with myocardial infarction were followed up under the conditions of systematic rehabilitation. They were tested by the veloergometer at the beginning of the fourth and seventh month post the coronary incident. The dynamics of the indices concerning the outer work and oxygen consumption of the myocardium was followed up. The author found that the indices of outer work (capacity of threshold loading and volume of the work carried out) reflect the improved physical capacity of the patients respectively. The dynamics of the indices for the heart work, myocardial oxygen consumption respis not so regular and puts certain problems forward. That fact demanded the inclusion of additional parameters, being revelant to the myocardial contractility. In this way, the rehabilitation regimen could be more precisely determined as well as the effectivity of the therapeutic measures carried out.", "contents": "[Dynamic changes in the indices of myocardial infarct patients at the polyclinical stage of rehabilitation]. Twenty five patients with myocardial infarction were followed up under the conditions of systematic rehabilitation. They were tested by the veloergometer at the beginning of the fourth and seventh month post the coronary incident. The dynamics of the indices concerning the outer work and oxygen consumption of the myocardium was followed up. The author found that the indices of outer work (capacity of threshold loading and volume of the work carried out) reflect the improved physical capacity of the patients respectively. The dynamics of the indices for the heart work, myocardial oxygen consumption respis not so regular and puts certain problems forward. That fact demanded the inclusion of additional parameters, being revelant to the myocardial contractility. In this way, the rehabilitation regimen could be more precisely determined as well as the effectivity of the therapeutic measures carried out."}
{"id": "PMID:475895", "title": "[Circulation immune complexes in chronic glomerulonephritis].", "content": "Thirty six patients with chronic glomerulonephritis, 35 -- with chronic pyelonephritis, 5 -- with diffuse glomerulonephritis with systemic lupus erythematosus and 60 healthy subjects were examined with the method by sedimentation with 3.5 per cent solution of polyethyleneglycol for the determination of circulating immune complexes. The average value for the healthy subjects is X = 0.123 +/- 0.047 mg/ml. The average value plus two standard deviations = 0.217 mg/ml is accepted as normal in healthy subjects. An elevated level of circulating immune complexes is found in 60 per cent of the patients with lupus nephritis and 25 per cent of the patients with chronic glomerulonephritis. The average values for the last two groups are X = 0.475 +/- 0.554 mg/ml and X = 0.184 +/- 0.185 mg/ml respectively. Whereas in the patients with lupus nephritis the values for the single patients are considerably over the adopted norm, in patients with chronic glomerulonephritis, in the majority of the cases, they are about its upper limit.", "contents": "[Circulation immune complexes in chronic glomerulonephritis]. Thirty six patients with chronic glomerulonephritis, 35 -- with chronic pyelonephritis, 5 -- with diffuse glomerulonephritis with systemic lupus erythematosus and 60 healthy subjects were examined with the method by sedimentation with 3.5 per cent solution of polyethyleneglycol for the determination of circulating immune complexes. The average value for the healthy subjects is X = 0.123 +/- 0.047 mg/ml. The average value plus two standard deviations = 0.217 mg/ml is accepted as normal in healthy subjects. An elevated level of circulating immune complexes is found in 60 per cent of the patients with lupus nephritis and 25 per cent of the patients with chronic glomerulonephritis. The average values for the last two groups are X = 0.475 +/- 0.554 mg/ml and X = 0.184 +/- 0.185 mg/ml respectively. Whereas in the patients with lupus nephritis the values for the single patients are considerably over the adopted norm, in patients with chronic glomerulonephritis, in the majority of the cases, they are about its upper limit."}
{"id": "PMID:475896", "title": "[Abdominal manifestations of chronic pyelonephritis].", "content": "The most frequent abdominal complaints in chronic pyelonephritis are analyzed and one patient is described, who with the exacerbation of the disease manifested the clinical picture of acute abdomen. The involvement of visceral-dermal reflexes with hyperalgesia, muscular defense, vomiting, meteroism and disturbances in intestinal motility imitated the picture of acute abdominal drama, forcing the surgical intervention. The varied abdominal symptomatics in about 20 per cent of the cases with chronic exacerbated pyelonephritis, should be critically assessed by the therapeutists.", "contents": "[Abdominal manifestations of chronic pyelonephritis]. The most frequent abdominal complaints in chronic pyelonephritis are analyzed and one patient is described, who with the exacerbation of the disease manifested the clinical picture of acute abdomen. The involvement of visceral-dermal reflexes with hyperalgesia, muscular defense, vomiting, meteroism and disturbances in intestinal motility imitated the picture of acute abdominal drama, forcing the surgical intervention. The varied abdominal symptomatics in about 20 per cent of the cases with chronic exacerbated pyelonephritis, should be critically assessed by the therapeutists."}
{"id": "PMID:475897", "title": "[Echocardiographic indices of the pumping and contractile functions of the left ventricle and the hemodynamic data obtained from left and/or right cardiac catheterization].", "content": "A juxtaposition of the EchoC-indices for the pump and contraction function of left ventricle and the hemodynamic data obtained with right and left cardiac catheterization was carried out in 95 patients with different myocardial and valvular heart diseases. The following EchoC-indices were determined for the assessment of the left-ventricle function -- telediastolic dimension and volume of left ventricle, stroke and minute heart volume, fraction of shortening (FS), average contraction speed of circumferential fibres (CCF), fraction of expulsion (FE), left auricle dimension. A considerable correlation was found among the EchoC-indices for pump and contraction function of left ventricle and the final diastolic left ventricle pressure and the average left auricle pressure. In heart patients with a normal final diastolic pressure in left ventricle and average left auricle pressure, pulmonary capillaries (PC) resp., the average values of all functional EchoC-indices are normal. With an elevated final diastolic pressure in left ventricle, diminished values of dP/dt max and elevated mean pressure in left auricle, the pulmonary capillaries resp., the values of all EchoC-indices for the pump and contraction left ventricle function are pathological -- enlarged telediastolic dimension and left ventricle volume, enlarged dimension of left ventricle, reduced values of FS, CCF, FE, SV and MV. EchoC-functional indices properly differentiate the pathological from the normal hemodynamics of left ventricle. determined by catheterization in 86 per cent of the cases. The false positive and false negative results are obtained in 14 per cent of the cases.", "contents": "[Echocardiographic indices of the pumping and contractile functions of the left ventricle and the hemodynamic data obtained from left and/or right cardiac catheterization]. A juxtaposition of the EchoC-indices for the pump and contraction function of left ventricle and the hemodynamic data obtained with right and left cardiac catheterization was carried out in 95 patients with different myocardial and valvular heart diseases. The following EchoC-indices were determined for the assessment of the left-ventricle function -- telediastolic dimension and volume of left ventricle, stroke and minute heart volume, fraction of shortening (FS), average contraction speed of circumferential fibres (CCF), fraction of expulsion (FE), left auricle dimension. A considerable correlation was found among the EchoC-indices for pump and contraction function of left ventricle and the final diastolic left ventricle pressure and the average left auricle pressure. In heart patients with a normal final diastolic pressure in left ventricle and average left auricle pressure, pulmonary capillaries (PC) resp., the average values of all functional EchoC-indices are normal. With an elevated final diastolic pressure in left ventricle, diminished values of dP/dt max and elevated mean pressure in left auricle, the pulmonary capillaries resp., the values of all EchoC-indices for the pump and contraction left ventricle function are pathological -- enlarged telediastolic dimension and left ventricle volume, enlarged dimension of left ventricle, reduced values of FS, CCF, FE, SV and MV. EchoC-functional indices properly differentiate the pathological from the normal hemodynamics of left ventricle. determined by catheterization in 86 per cent of the cases. The false positive and false negative results are obtained in 14 per cent of the cases."}
{"id": "PMID:475898", "title": "[Bicuspid aortic valve. Echocardiographic diagnostic potentials].", "content": "The echocardiographic potentialities in the diagnosis of bicuspid aortic valve are discussed. In the group of 32 patients examined with a clinic and murmurs suspected for that disease, the index of excentricity in five patients, was found to be respectively 1.6, 1.4, 1.6, 1.8, 1.5, accepted as a reliable index for the diagnosis of aortic bicuspidia. Excentricity index is equal to 1/2 of the aortic diameter, divided to the minimal distance from the diastolic echo of the closed aortic valve to the closer aortic wall. The control group consisted of 44 patients with tricuspid aortic valve, 17 of them being with aortic defect (isolated stenosis or combined aortic defect) and the rest 28 -- without valvular heart disease. Excentricity index for the control group was low -- 1.0--1.33 (average value 1.28). Conclusions are drawn that the echocardiographic method, from the group of the bloodless methods, is with the best diagnostic potentialities in the bicuspid valve and should be looked for in patients with expulsion murmur and click of aortic origin.", "contents": "[Bicuspid aortic valve. Echocardiographic diagnostic potentials]. The echocardiographic potentialities in the diagnosis of bicuspid aortic valve are discussed. In the group of 32 patients examined with a clinic and murmurs suspected for that disease, the index of excentricity in five patients, was found to be respectively 1.6, 1.4, 1.6, 1.8, 1.5, accepted as a reliable index for the diagnosis of aortic bicuspidia. Excentricity index is equal to 1/2 of the aortic diameter, divided to the minimal distance from the diastolic echo of the closed aortic valve to the closer aortic wall. The control group consisted of 44 patients with tricuspid aortic valve, 17 of them being with aortic defect (isolated stenosis or combined aortic defect) and the rest 28 -- without valvular heart disease. Excentricity index for the control group was low -- 1.0--1.33 (average value 1.28). Conclusions are drawn that the echocardiographic method, from the group of the bloodless methods, is with the best diagnostic potentialities in the bicuspid valve and should be looked for in patients with expulsion murmur and click of aortic origin."}
{"id": "PMID:475899", "title": "[Ischemic heart disease and embolisms and thomboses in the lesser circulation].", "content": "All deceased with ischemic heart disease (IHD) -- 450 cases and with embolism and thrombosis in pulmonary circulation (ETPC) -- 601 cases were purposefully examined for a period of 10 years (1963--1972). In 75 of the examined a combination of IHD and ETPC was present. In 41 out of them only cicatrices of past infarctions were found and in 34 -- recent myocardial infarctions. In 14 of the examined (41%) it was established, clinically and morphologically, that ETPC are with a longer duration than the recent myocardial infarctions and very likely have played a role in their pathogenesis. The confirmation of acute ischemic changes in myocardium gives a support -- undulation of the myofibrils and fuchsinophylic necrosis in the deceased of ETPC without preceeding IHD. The cardiac hypertrophy and coronary atherosclerosis lead to an intensification of the myocardial ischemic alterations in ETPC.", "contents": "[Ischemic heart disease and embolisms and thomboses in the lesser circulation]. All deceased with ischemic heart disease (IHD) -- 450 cases and with embolism and thrombosis in pulmonary circulation (ETPC) -- 601 cases were purposefully examined for a period of 10 years (1963--1972). In 75 of the examined a combination of IHD and ETPC was present. In 41 out of them only cicatrices of past infarctions were found and in 34 -- recent myocardial infarctions. In 14 of the examined (41%) it was established, clinically and morphologically, that ETPC are with a longer duration than the recent myocardial infarctions and very likely have played a role in their pathogenesis. The confirmation of acute ischemic changes in myocardium gives a support -- undulation of the myofibrils and fuchsinophylic necrosis in the deceased of ETPC without preceeding IHD. The cardiac hypertrophy and coronary atherosclerosis lead to an intensification of the myocardial ischemic alterations in ETPC."}
{"id": "PMID:475900", "title": "[Excretion of uric acid in cardiac decompensation].", "content": "The role of hyperuricemia is confirmed, in literature, as a risk factor in ischemic heart disease and in the development of atherosclerotic complications. The problem of uric acid concentration in serum is still not elucidated as well as the role of the eventual hyperuricemia as a risk factor and its excretion from the kidneys of patients with heart diseases in a decompensation stage. Thirty five patients with different heart diseases in various decompensation stages were observed. In all patients, the uric acid concentration in serum were studied as well as the clearance of uric acid, reabsorption percentage in tubules and glomerular filtration according to the clearance of endogenous creatinine. The results obtained revealed that hyperuricemia (over 7 mg%) is found in a great number of the patients (40%) with heart decompensation. The hyperuricemia established resulted primarily from the reduced filtration of glomeruli. In some of the cases, an increased reabsorption of uric acid in tubules was found. In other, single cases, data exist about uric acid secretion in tubules. The problem of heart patients treatment in decompensation stage with the presence of hyperuricemia is discussed. In those cases, very likely, prophylactic measures should be undertaken due to the fact that hyperuricemia is a factor, secondarily leading to atherosclerotic heart alterations.", "contents": "[Excretion of uric acid in cardiac decompensation]. The role of hyperuricemia is confirmed, in literature, as a risk factor in ischemic heart disease and in the development of atherosclerotic complications. The problem of uric acid concentration in serum is still not elucidated as well as the role of the eventual hyperuricemia as a risk factor and its excretion from the kidneys of patients with heart diseases in a decompensation stage. Thirty five patients with different heart diseases in various decompensation stages were observed. In all patients, the uric acid concentration in serum were studied as well as the clearance of uric acid, reabsorption percentage in tubules and glomerular filtration according to the clearance of endogenous creatinine. The results obtained revealed that hyperuricemia (over 7 mg%) is found in a great number of the patients (40%) with heart decompensation. The hyperuricemia established resulted primarily from the reduced filtration of glomeruli. In some of the cases, an increased reabsorption of uric acid in tubules was found. In other, single cases, data exist about uric acid secretion in tubules. The problem of heart patients treatment in decompensation stage with the presence of hyperuricemia is discussed. In those cases, very likely, prophylactic measures should be undertaken due to the fact that hyperuricemia is a factor, secondarily leading to atherosclerotic heart alterations."}
{"id": "PMID:475902", "title": "[Malignant, rapidly progressing nephritis in Sch\u00f6nlein-Henoch disease].", "content": "A case of malignant quickly progressing Henoch-nephritis is described, with a lethal end one and a half month after the initial manifestations. The disease develops with a clinical picture typical for systemic vasculitis, with joint-pain, skin-rash and grave abdominal syndrome, being the reason for unnecessary surgical intervention. The renal changes originate even at the very beginning of the disease and are clinically characterized, besides the exceptionally quick evolution, by the picture of acute renal insufficiency with progressing azotemia, severe electrolyte disturbances and anemic syndrome and pathomorphologically by the picture of severe diffuse intra- and extracapillary proliferative glomerulitis. The treatment with antihistamine preparations, heparin and corticosteroids failed to give an effect.", "contents": "[Malignant, rapidly progressing nephritis in Sch\u00f6nlein-Henoch disease]. A case of malignant quickly progressing Henoch-nephritis is described, with a lethal end one and a half month after the initial manifestations. The disease develops with a clinical picture typical for systemic vasculitis, with joint-pain, skin-rash and grave abdominal syndrome, being the reason for unnecessary surgical intervention. The renal changes originate even at the very beginning of the disease and are clinically characterized, besides the exceptionally quick evolution, by the picture of acute renal insufficiency with progressing azotemia, severe electrolyte disturbances and anemic syndrome and pathomorphologically by the picture of severe diffuse intra- and extracapillary proliferative glomerulitis. The treatment with antihistamine preparations, heparin and corticosteroids failed to give an effect."}
{"id": "PMID:475910", "title": "[Particular ultrastructural aspects of human Paneth's cells].", "content": "Our research is about the ultrastructural aspects of Paneth cells obtained from human jejunal mucosa. The cells appear columnar in shape and show a roundish nucleus; their cytoplasm is characterized by a great amount of R.E.R. and by typical large granules in sopranuclear position. Our results confirm those previously described by other authors about the morphology of Paneth cells. In our specimens we have found moreover a variable number of particular cytoplasmic structures; these may show a fibrillar or granular matrix and are always surrounded by a membrane.", "contents": "[Particular ultrastructural aspects of human Paneth's cells]. Our research is about the ultrastructural aspects of Paneth cells obtained from human jejunal mucosa. The cells appear columnar in shape and show a roundish nucleus; their cytoplasm is characterized by a great amount of R.E.R. and by typical large granules in sopranuclear position. Our results confirm those previously described by other authors about the morphology of Paneth cells. In our specimens we have found moreover a variable number of particular cytoplasmic structures; these may show a fibrillar or granular matrix and are always surrounded by a membrane."}
{"id": "PMID:475911", "title": "[Prolactin after repeated intravenous injections of cimetidine].", "content": "To 4 normal women a bolus of mg 400 of cimetidine was injected intravenously once an hour for three hours in succession and plasma prolactin behaviour studied. No depletion in prolactin pituitary storage was observed in all subjects. The possible usefulness of cimetidine for clinical diagnostic purposes is discussed.", "contents": "[Prolactin after repeated intravenous injections of cimetidine]. To 4 normal women a bolus of mg 400 of cimetidine was injected intravenously once an hour for three hours in succession and plasma prolactin behaviour studied. No depletion in prolactin pituitary storage was observed in all subjects. The possible usefulness of cimetidine for clinical diagnostic purposes is discussed."}
{"id": "PMID:475912", "title": "A multi-channel, tele-metric system for analysing biological data: extern work, the electrocardiogram, etc...", "content": "A new multichannel, telemetric system for analysing biological data was developed and tested, in collaboration with the Berta Firm in Turin. This system was tested on subjects at 4650 meters in Peru. The results obtained show the usefulness of this tool. In addition it is very good in evaluating the progress of post-myocardial infarctuated patients.", "contents": "A multi-channel, tele-metric system for analysing biological data: extern work, the electrocardiogram, etc... A new multichannel, telemetric system for analysing biological data was developed and tested, in collaboration with the Berta Firm in Turin. This system was tested on subjects at 4650 meters in Peru. The results obtained show the usefulness of this tool. In addition it is very good in evaluating the progress of post-myocardial infarctuated patients."}
{"id": "PMID:475913", "title": "Thoracic and pulmonary radiographic modification during immersion in apnea: 1--a special apparatus.", "content": "Thoracic and pulmonary radiographic modification during immersion in apnea, were studied. An Apparatus \"Nuclearisub\" was used, it consists of two parts: An x-ray generator (100 KV, 40 mA) and a image intensifier tube (brightness gain 1200) with a transistorized telecamera. Through continuous radioscopy during immersion, can be analyzed the variations caused by redistribution of the blood, as well as the changes in pressure.", "contents": "Thoracic and pulmonary radiographic modification during immersion in apnea: 1--a special apparatus. Thoracic and pulmonary radiographic modification during immersion in apnea, were studied. An Apparatus \"Nuclearisub\" was used, it consists of two parts: An x-ray generator (100 KV, 40 mA) and a image intensifier tube (brightness gain 1200) with a transistorized telecamera. Through continuous radioscopy during immersion, can be analyzed the variations caused by redistribution of the blood, as well as the changes in pressure."}
{"id": "PMID:475916", "title": "[Experimental poisoning with gaseous hydrocarbons: changes in concentrations of propane and butane in the lung and adipose tissue in relation to the time of death].", "content": "Postmortal changes of propane, butane and iso-butane concentration in the lung and fat of mice exposed to lethal intoxication with commercial liquid gas are studied. The hydrocarbon tissue concentrations after exposure were determined by gas liquid chromatography. The hydrocarbons progressively decreased in the lung and disappeared, depending on the degree of putrefaction; even in case of remarkable putrefaction, it was still possible to detect them in the fat tissue.", "contents": "[Experimental poisoning with gaseous hydrocarbons: changes in concentrations of propane and butane in the lung and adipose tissue in relation to the time of death]. Postmortal changes of propane, butane and iso-butane concentration in the lung and fat of mice exposed to lethal intoxication with commercial liquid gas are studied. The hydrocarbon tissue concentrations after exposure were determined by gas liquid chromatography. The hydrocarbons progressively decreased in the lung and disappeared, depending on the degree of putrefaction; even in case of remarkable putrefaction, it was still possible to detect them in the fat tissue."}
{"id": "PMID:475918", "title": "Effect of acute arterio-venous fistulae on mean and phasic coronary flow in the dog.", "content": "The effect of acute arterio-venous fistula on mean and phasic coronary flow was studied on 4 open-chest dogs. When the pressure is basically high, after the opening of the fistula the fall which coronary flow shows during the isometric systole occurs to a lesser extent; conversely, when the pressure is basically normal, the fall of the coronary flow may be absent.", "contents": "Effect of acute arterio-venous fistulae on mean and phasic coronary flow in the dog. The effect of acute arterio-venous fistula on mean and phasic coronary flow was studied on 4 open-chest dogs. When the pressure is basically high, after the opening of the fistula the fall which coronary flow shows during the isometric systole occurs to a lesser extent; conversely, when the pressure is basically normal, the fall of the coronary flow may be absent."}
{"id": "PMID:475919", "title": "[Cholinesterase of the chick optic lobe. Molecular forms and behavior after deafferentation].", "content": "The behaviour of AChE and ChE has been studied quantitatively in the chick optic lobe (tectum and nuclei) under normal conditions and after deafferentation. Eye extirpation was carried out at the 1st day after hatching. Both in the tectum and in the nuclei, a low decrease in specific activity was observed, in comparison with the control. Nevertheless, when the total activity is calculated, a significant decrease is evident, owing to marked underdevelopment of the deafferented lobe. In the normal chick lobe, 2 molecular forms of AChE (6.58 and 11S) have been observed. The distribution of these 2 forms is not altered by eye extirpation, until at least 120 days of age.", "contents": "[Cholinesterase of the chick optic lobe. Molecular forms and behavior after deafferentation]. The behaviour of AChE and ChE has been studied quantitatively in the chick optic lobe (tectum and nuclei) under normal conditions and after deafferentation. Eye extirpation was carried out at the 1st day after hatching. Both in the tectum and in the nuclei, a low decrease in specific activity was observed, in comparison with the control. Nevertheless, when the total activity is calculated, a significant decrease is evident, owing to marked underdevelopment of the deafferented lobe. In the normal chick lobe, 2 molecular forms of AChE (6.58 and 11S) have been observed. The distribution of these 2 forms is not altered by eye extirpation, until at least 120 days of age."}
{"id": "PMID:475920", "title": "Evidence for heterogeneous turnover of rat liver lysosome hydrolases.", "content": "The activity of several Lysosome Hydrolases in the liver of rats submitted to intensive treatment with cycloheximide has been tested. From their results the authors suggest that the lysosomal turnover is not only heterogenous but also rapid.", "contents": "Evidence for heterogeneous turnover of rat liver lysosome hydrolases. The activity of several Lysosome Hydrolases in the liver of rats submitted to intensive treatment with cycloheximide has been tested. From their results the authors suggest that the lysosomal turnover is not only heterogenous but also rapid."}
{"id": "PMID:475921", "title": "Inhibition of liver protein degradation in vivo by intensive treatment with cycloheximide.", "content": "The AA in experiments performed on male rats treated with 0,5 mCi/kg of cycloheximide have observed an inhibition of liver protein degradation and have suggested two mechanism: 1) cycloheximide prevents the acute proteolytic response induced in fibroblast culture by exposure to serum-deficient media and in rat livers after perfusion probably by inhibiting the lysosomal - autophagic system, without modifications in total activity of lysosome proteinases. 2) It may be that protein degradation rate and level of lysosomal proteinases reinterrelated; the latter probably being a factor controlling the overall cell protein turnover rate.", "contents": "Inhibition of liver protein degradation in vivo by intensive treatment with cycloheximide. The AA in experiments performed on male rats treated with 0,5 mCi/kg of cycloheximide have observed an inhibition of liver protein degradation and have suggested two mechanism: 1) cycloheximide prevents the acute proteolytic response induced in fibroblast culture by exposure to serum-deficient media and in rat livers after perfusion probably by inhibiting the lysosomal - autophagic system, without modifications in total activity of lysosome proteinases. 2) It may be that protein degradation rate and level of lysosomal proteinases reinterrelated; the latter probably being a factor controlling the overall cell protein turnover rate."}
{"id": "PMID:475922", "title": "Fluctuations in the level of liver cathepsins after partial hepatectomy.", "content": "Fluctuations in the level of liver cathepsins after partial hepatectomy were studied. The reduction in proteinase activities in dividing cells might conceivably reveal larger than that measured on the whole tissue since no more than 40-50% of residual liver cells takes part into the first mitotic wave after partial hepatectomy.", "contents": "Fluctuations in the level of liver cathepsins after partial hepatectomy. Fluctuations in the level of liver cathepsins after partial hepatectomy were studied. The reduction in proteinase activities in dividing cells might conceivably reveal larger than that measured on the whole tissue since no more than 40-50% of residual liver cells takes part into the first mitotic wave after partial hepatectomy."}
{"id": "PMID:475925", "title": "Acetylcholine's inhibitory effect on the calcium-dependent depolarization in the heart cells of a reptile (Lacerta sicula campestris). Preliminary notes.", "content": "The results of experiments performed with high - K+ solutions suggest that in the lizard atrial cells Ach normally displays its negative inotropic effects by a double mechanism = an \"indirect\" inhibition, mediated by the increase of the membrane permeability to K+ ions; and a \"direct\" inhibition of the Ca- carried slow inward current. In the ventricular fibres there isn't a direct-effect of this drug, under normal conditions.", "contents": "Acetylcholine's inhibitory effect on the calcium-dependent depolarization in the heart cells of a reptile (Lacerta sicula campestris). Preliminary notes. The results of experiments performed with high - K+ solutions suggest that in the lizard atrial cells Ach normally displays its negative inotropic effects by a double mechanism = an \"indirect\" inhibition, mediated by the increase of the membrane permeability to K+ ions; and a \"direct\" inhibition of the Ca- carried slow inward current. In the ventricular fibres there isn't a direct-effect of this drug, under normal conditions."}
{"id": "PMID:475927", "title": "Uncontrolled ventricular rate in atrial fibrillation. A manifestation of dissimilar atrial rhythms.", "content": "A patient with coarse atrial fibrillation and a rapid ventricular response developed periods of high grade atrioventricular block interpersed with periods of rapid ventricular conduction after the administration of digitalis and propranolol. Intracardiac atrial recordings showed similar atrial rhythms of high right atrial flutter and left atrial fibrillation. The low right atrial recordings showed flutter during the periods of fast ventricular rates and fibrillation during periods of slower ventricular rates.", "contents": "Uncontrolled ventricular rate in atrial fibrillation. A manifestation of dissimilar atrial rhythms. A patient with coarse atrial fibrillation and a rapid ventricular response developed periods of high grade atrioventricular block interpersed with periods of rapid ventricular conduction after the administration of digitalis and propranolol. Intracardiac atrial recordings showed similar atrial rhythms of high right atrial flutter and left atrial fibrillation. The low right atrial recordings showed flutter during the periods of fast ventricular rates and fibrillation during periods of slower ventricular rates."}
{"id": "PMID:475928", "title": "Coronary artery spasm of different degrees as cause of angina at rest with ST segment depression and elevation.", "content": "Chest pain induced by ergometrine was associated with ST depression and subsequent elevation in anterior leads in a 42-year-old man suffering from angina at rest. Coronary arteriography during the attack showed that coronary arterial spasm of different degrees was responsible for chest pain and both types of electrocardiographic abnormality.", "contents": "Coronary artery spasm of different degrees as cause of angina at rest with ST segment depression and elevation. Chest pain induced by ergometrine was associated with ST depression and subsequent elevation in anterior leads in a 42-year-old man suffering from angina at rest. Coronary arteriography during the attack showed that coronary arterial spasm of different degrees was responsible for chest pain and both types of electrocardiographic abnormality."}
{"id": "PMID:475929", "title": "Echocardiographic diagnosis of mobile left ventricular tumour.", "content": "Echocardiography detected a mobile left ventricular tumour. The tracing showed a cluster of echoes in the left ventricular outflow tract corresponding to the location of the tumour as confirmed by cineangiograms. At operation, a thrombus was discovered, attached to a chorda tendineae.", "contents": "Echocardiographic diagnosis of mobile left ventricular tumour. Echocardiography detected a mobile left ventricular tumour. The tracing showed a cluster of echoes in the left ventricular outflow tract corresponding to the location of the tumour as confirmed by cineangiograms. At operation, a thrombus was discovered, attached to a chorda tendineae."}
{"id": "PMID:475930", "title": "Double outlet from chambers of left ventricular morphology.", "content": "This series of 5 cases with double outlet of morphologically left ventricular chamber includes 4 found during a review of 1700 heart specimens (incidence 0.23%) and 1 found at operation and successfully corrected. Abnormal atrioventricular connection precluding total correction was present in the 4 anatomical cases. Clinical diagnosis may be difficult and it is suggested that axial cineangiography may make anatomical diagnosis easier. Absence of the infundibular septum and aortic laevoposition are frequent. As some cases can be surgically corrected, accurate information is required on the size of the right ventricle, the morphology and function of the atrioventricular valves, the presence, size, and position of the ventricular septal defect, and the degree and type of outflow tract obstruction.", "contents": "Double outlet from chambers of left ventricular morphology. This series of 5 cases with double outlet of morphologically left ventricular chamber includes 4 found during a review of 1700 heart specimens (incidence 0.23%) and 1 found at operation and successfully corrected. Abnormal atrioventricular connection precluding total correction was present in the 4 anatomical cases. Clinical diagnosis may be difficult and it is suggested that axial cineangiography may make anatomical diagnosis easier. Absence of the infundibular septum and aortic laevoposition are frequent. As some cases can be surgically corrected, accurate information is required on the size of the right ventricle, the morphology and function of the atrioventricular valves, the presence, size, and position of the ventricular septal defect, and the degree and type of outflow tract obstruction."}
{"id": "PMID:475931", "title": "Two-chambered right ventricle: simulating two-chambered left ventricle.", "content": "Two cases are described of a most unusual variant of two-chambered right ventricle. In both the ventricular septal defect was between the distal chamber of the right ventricle and the left ventricle. However the extensive dividing 'septum' between proximal and distal parts of the right ventricle converted the latter, haemodynamically, into part of the left ventricle. In the first case the distal chamber supported the aorta in the left anterior position, the pulmonary artery arising from the proximal part of the right ventricle. In the second the pulmonary artery arose from the distal chamber and the aorta from the proximal chamber. Though in both the ventriculoarterial connection was double outlet right ventricle, functionally there was arterial concordance in case 1 and discordance in case 2. A further disconcerting feature was the resemblance of the distal right ventricular chamber to the rudimentary chamber of a univentricular heart of left ventricular type.", "contents": "Two-chambered right ventricle: simulating two-chambered left ventricle. Two cases are described of a most unusual variant of two-chambered right ventricle. In both the ventricular septal defect was between the distal chamber of the right ventricle and the left ventricle. However the extensive dividing 'septum' between proximal and distal parts of the right ventricle converted the latter, haemodynamically, into part of the left ventricle. In the first case the distal chamber supported the aorta in the left anterior position, the pulmonary artery arising from the proximal part of the right ventricle. In the second the pulmonary artery arose from the distal chamber and the aorta from the proximal chamber. Though in both the ventriculoarterial connection was double outlet right ventricle, functionally there was arterial concordance in case 1 and discordance in case 2. A further disconcerting feature was the resemblance of the distal right ventricular chamber to the rudimentary chamber of a univentricular heart of left ventricular type."}
{"id": "PMID:475933", "title": "Haemodynamic effects of dobutamine with special reference to myocardial blood flow. A comparison with dopamine and isoprenaline.", "content": "The haemodynamic effects of dobutamine (2.5 to 10 micrograms/min per kg) were determined in 5 patients without cardiac failure who were undergoing cardiac catheterisation for suspected coronary disease. Myocardial blood flow was determined by the coronary sinus thermodilution technique. Data were compared with those from two groups of 5 patients who received dopamine (4-8 micrograms/min per kg) and isoprenaline (0.005-0.025 micrograms/min per kg). Each drug was given in a lower and a higher dose, and all increased mean cardiac index (dobutamine, 18% and 39%; dopamine, 11% and 23%; isoprenaline, 15% and 44%). These increases were associated with significant increases in mean myocardial oxygen consumption (dobutamine, 38% and 61%; dopamine, 25% and 62%; isoprenaline, 20% and 45%). Mean myocardial blood flow was increased by each drug but mean myocardial oxygen extraction was decreased by isoprenaline, was increased by dopamine, and was unchanged by dobutamine. Each inotropic agent has a similar effect on myocardial oxygen consumption, but isoprenaline has a direct coronary vasodilator action while dopamine has a coronary vasoconstrictor action. Dobutamine has no direct effect upon coronary vascular tone.", "contents": "Haemodynamic effects of dobutamine with special reference to myocardial blood flow. A comparison with dopamine and isoprenaline. The haemodynamic effects of dobutamine (2.5 to 10 micrograms/min per kg) were determined in 5 patients without cardiac failure who were undergoing cardiac catheterisation for suspected coronary disease. Myocardial blood flow was determined by the coronary sinus thermodilution technique. Data were compared with those from two groups of 5 patients who received dopamine (4-8 micrograms/min per kg) and isoprenaline (0.005-0.025 micrograms/min per kg). Each drug was given in a lower and a higher dose, and all increased mean cardiac index (dobutamine, 18% and 39%; dopamine, 11% and 23%; isoprenaline, 15% and 44%). These increases were associated with significant increases in mean myocardial oxygen consumption (dobutamine, 38% and 61%; dopamine, 25% and 62%; isoprenaline, 20% and 45%). Mean myocardial blood flow was increased by each drug but mean myocardial oxygen extraction was decreased by isoprenaline, was increased by dopamine, and was unchanged by dobutamine. Each inotropic agent has a similar effect on myocardial oxygen consumption, but isoprenaline has a direct coronary vasodilator action while dopamine has a coronary vasoconstrictor action. Dobutamine has no direct effect upon coronary vascular tone."}
{"id": "PMID:475935", "title": "Age, sex, and ischaemic heart disease as prognostic indicators in long-term cardiac pacing.", "content": "The influence of age, sex, and overt ischaemic heart disease on survival after pacing was studied in a population of 427 patients. The median survival for the whole group was 7.75 years. Women between 55 and 74 years and men between 65 and 74 years enjoyed median survivals in excess of 10 years. Patients over 74 years and men with ischaemic heart disease had median survivals of the order of 5 years and 3 1/2 years, respectively. The findings may help to match generator lifetimes to patient-survival prospects.", "contents": "Age, sex, and ischaemic heart disease as prognostic indicators in long-term cardiac pacing. The influence of age, sex, and overt ischaemic heart disease on survival after pacing was studied in a population of 427 patients. The median survival for the whole group was 7.75 years. Women between 55 and 74 years and men between 65 and 74 years enjoyed median survivals in excess of 10 years. Patients over 74 years and men with ischaemic heart disease had median survivals of the order of 5 years and 3 1/2 years, respectively. The findings may help to match generator lifetimes to patient-survival prospects."}
{"id": "PMID:475937", "title": "Persistence and effects of sinus rhythm after Fontan procedure for tricuspid atresia.", "content": "Four patients who had had a Fontan type of procedure for tricuspid atresia 23, 6, 6, and 11 months previously were investigated by ambulatory electrocardiographic recording and simultaneous recording of the jugular venous pressure and echocardiogram of the conduit or pulmonary valve. All had been considerably improved by the operation. In 1 patient episodes of supraventricular tachycardia were recorded but no rhythm disturbance was detected in the other 3. Pulmonary blood flow was shown to be pulsatile and atrial systole is an important factor in this. The conduit valve showed delayed opening and slow closure suggesting that its presence in the pulmonary circuit may be unnecessary.", "contents": "Persistence and effects of sinus rhythm after Fontan procedure for tricuspid atresia. Four patients who had had a Fontan type of procedure for tricuspid atresia 23, 6, 6, and 11 months previously were investigated by ambulatory electrocardiographic recording and simultaneous recording of the jugular venous pressure and echocardiogram of the conduit or pulmonary valve. All had been considerably improved by the operation. In 1 patient episodes of supraventricular tachycardia were recorded but no rhythm disturbance was detected in the other 3. Pulmonary blood flow was shown to be pulsatile and atrial systole is an important factor in this. The conduit valve showed delayed opening and slow closure suggesting that its presence in the pulmonary circuit may be unnecessary."}
{"id": "PMID:475938", "title": "Prinzmetal's angina:reflex cardiovascular response during episode of pain.", "content": "Previous angiographic studies have shown that coronary spasm occurs in association with the variant angina described by Prinzmetal, confirming his original hypothesis. In this work we recorded the heart rate changes and the incidence of arrhythmias during variant angina. The patients were divided into two groups: anterior, with electrocardiographic signs of anterior ischaemia, and inferior, with changes in the inferior leads. There was a significant increase of heart rate during pain in anterior myocardial ischaemia and a significant decrease when the ischaemia was inferior. The incidence of ectopic arrhythmias during pain was significantly greater in patients with anterior ischaemia, but there was a high incidence of atrioventricular block in patients with inferior ischaemia. We suggest that these findings can be explained by different responses of the automatic nervous system to anterior and inferior acute myocardial ischaemia.", "contents": "Prinzmetal's angina:reflex cardiovascular response during episode of pain. Previous angiographic studies have shown that coronary spasm occurs in association with the variant angina described by Prinzmetal, confirming his original hypothesis. In this work we recorded the heart rate changes and the incidence of arrhythmias during variant angina. The patients were divided into two groups: anterior, with electrocardiographic signs of anterior ischaemia, and inferior, with changes in the inferior leads. There was a significant increase of heart rate during pain in anterior myocardial ischaemia and a significant decrease when the ischaemia was inferior. The incidence of ectopic arrhythmias during pain was significantly greater in patients with anterior ischaemia, but there was a high incidence of atrioventricular block in patients with inferior ischaemia. We suggest that these findings can be explained by different responses of the automatic nervous system to anterior and inferior acute myocardial ischaemia."}
{"id": "PMID:475940", "title": "Atresia of the right atrioventricular orifice with atrioventricular concordance.", "content": "Three hearts are described in which a fibrous membrane was interposed between the right atrium and a formed but hypoplastic right ventricle, which possessed recognisable inlet, trabecular, and infundibular components. In these hearts the distribution of the conducting tissue was as expected for concordant atrioventricular connections, and contrasts with that seen in the classical type of 'tricuspid atresia'. The distinctive morphological and histological features of these specimens lend further support to our view that the majority of cases of atresia of the right atrioventricular orifice should be regarded as coming within the designation of 'the univentricular heart'.", "contents": "Atresia of the right atrioventricular orifice with atrioventricular concordance. Three hearts are described in which a fibrous membrane was interposed between the right atrium and a formed but hypoplastic right ventricle, which possessed recognisable inlet, trabecular, and infundibular components. In these hearts the distribution of the conducting tissue was as expected for concordant atrioventricular connections, and contrasts with that seen in the classical type of 'tricuspid atresia'. The distinctive morphological and histological features of these specimens lend further support to our view that the majority of cases of atresia of the right atrioventricular orifice should be regarded as coming within the designation of 'the univentricular heart'."}
{"id": "PMID:475941", "title": "Truncus arteriosus communis with intact ventricular septum.", "content": "This is the first documented case of truncus arteriosus communis with intact ventricular septum in which extensive clinical, haemodynamic, angiographic, and pathological data are available. Angiography suggested the presence of two discrete semilunar valves but necropsy showed a basically single semilunar valve. This case fills a gap in the spectrum of aorticopulmonary, truncal, and infundibular septal defects, and reinforces the belief that the essence of truncus arteriosus communis is a single semilunar valve, common to both ventricles, which need not be associated with the defects in the adjacent parts of the structural continuum.", "contents": "Truncus arteriosus communis with intact ventricular septum. This is the first documented case of truncus arteriosus communis with intact ventricular septum in which extensive clinical, haemodynamic, angiographic, and pathological data are available. Angiography suggested the presence of two discrete semilunar valves but necropsy showed a basically single semilunar valve. This case fills a gap in the spectrum of aorticopulmonary, truncal, and infundibular septal defects, and reinforces the belief that the essence of truncus arteriosus communis is a single semilunar valve, common to both ventricles, which need not be associated with the defects in the adjacent parts of the structural continuum."}
{"id": "PMID:475944", "title": "Drugs and human memory: effects of low doses of nitrazepam and hyoscine on retention.", "content": "1 The effects of diazepam (5 mg) and hyoscine hydrobromide (0.3 mg) were assessed in two memory tasks: short-term retention of digit strings and the free recall of items from categorizable lists. 2 One hundred and two healthy subjects were tested in an independent-groups design. Subjects were assigned randomly to either placebo, diazepam or hyoscine groups. Treatments were administered orally under double-blind conditions. 3 The short-term retention of digits was impaired to an equivalent degree and locus for both drugs (P less than 0.05). This effect was ascribed to the action on primary memory. 4 The drugs produced no significant effects on the recall of categorizable lists either in terms of the number of words recalled or the cohesiveness of categorical recall. 5 These results demonstrate that drugs of different pharmacological actions produce isomorphic psychological deficits in memory and that 'anti-memory' effects on one task should not be extrapolated to all aspects of memory.", "contents": "Drugs and human memory: effects of low doses of nitrazepam and hyoscine on retention. 1 The effects of diazepam (5 mg) and hyoscine hydrobromide (0.3 mg) were assessed in two memory tasks: short-term retention of digit strings and the free recall of items from categorizable lists. 2 One hundred and two healthy subjects were tested in an independent-groups design. Subjects were assigned randomly to either placebo, diazepam or hyoscine groups. Treatments were administered orally under double-blind conditions. 3 The short-term retention of digits was impaired to an equivalent degree and locus for both drugs (P less than 0.05). This effect was ascribed to the action on primary memory. 4 The drugs produced no significant effects on the recall of categorizable lists either in terms of the number of words recalled or the cohesiveness of categorical recall. 5 These results demonstrate that drugs of different pharmacological actions produce isomorphic psychological deficits in memory and that 'anti-memory' effects on one task should not be extrapolated to all aspects of memory."}
{"id": "PMID:475945", "title": "The activity of aryl hydrocarbon hydroxylase in adult human skin.", "content": "1 Aryl hydrocarbon hydroxylase (AHH), a mixed-function oxidase system, has been identified in microsomal preparations of adult breast skin and foreskin. 2 Separation of dermis from epidermis by stretching, showed that AHH activity in human skin is almost exclusively located within the epidermis. 3 Preincubation of whole chopped skin with benzanthracene (5 muM to 100 muM) using a tissue culture system was accompanied by a concentration dependent increase in AHH activity. 4 Although there was no significant difference in AHH activity between the two sites, individuals differed markedly from one another in activity at any one site. Activity was observed to be positively correlated with age.", "contents": "The activity of aryl hydrocarbon hydroxylase in adult human skin. 1 Aryl hydrocarbon hydroxylase (AHH), a mixed-function oxidase system, has been identified in microsomal preparations of adult breast skin and foreskin. 2 Separation of dermis from epidermis by stretching, showed that AHH activity in human skin is almost exclusively located within the epidermis. 3 Preincubation of whole chopped skin with benzanthracene (5 muM to 100 muM) using a tissue culture system was accompanied by a concentration dependent increase in AHH activity. 4 Although there was no significant difference in AHH activity between the two sites, individuals differed markedly from one another in activity at any one site. Activity was observed to be positively correlated with age."}
{"id": "PMID:475946", "title": "Phenytoin absorption in patients with ileojejunal bypass.", "content": "1 The absorption and elimination of phenytoin was studied after a 200 mg oral dose was administered to seven patients with ileojejunal bypass and nine control patients. 2 Analysis of the area under the plasma concentration time curve showed the absorption of phenytoin was decreased in the subjects with ileojejunal bypass (P less than 0.005). 3 The half life of elimination of the drug was shorter in the bypass group (15.1 h) than in the controls (17.8 h, P less than 0.05). 4 Subjects with an ileojejunal bypass receiving phenytoin are likely to require increased oral dosages to achieve an optimal plasma concentration. There is probably malabsorption of other poorly soluble drugs and parenteral therapy may be necessary to ensure adequate bioavailability.", "contents": "Phenytoin absorption in patients with ileojejunal bypass. 1 The absorption and elimination of phenytoin was studied after a 200 mg oral dose was administered to seven patients with ileojejunal bypass and nine control patients. 2 Analysis of the area under the plasma concentration time curve showed the absorption of phenytoin was decreased in the subjects with ileojejunal bypass (P less than 0.005). 3 The half life of elimination of the drug was shorter in the bypass group (15.1 h) than in the controls (17.8 h, P less than 0.05). 4 Subjects with an ileojejunal bypass receiving phenytoin are likely to require increased oral dosages to achieve an optimal plasma concentration. There is probably malabsorption of other poorly soluble drugs and parenteral therapy may be necessary to ensure adequate bioavailability."}
{"id": "PMID:475947", "title": "The effect of food and tablet formulation on plasma prednisolone levels following administration of enteric-coated tablets.", "content": "1 Plasma prednisolone levels have been compared following the administration of enteric-coated prednisolone to fasted and non-fasted subjects. The effect on plasma levels of altering the formulation of the enteric-coating has also been studied. 2 The presence of food in the stomach at the time of administration does not affect the absorption of enteric-coated prednisolone tablets. 3 There was considerable inter-subject variation in plasma prednisolone levels after administration of shellac based enteric-coated tablets. However, plasma levels were more consistent when a preparation whose formulation was based upon cellulose acetate phthalate (CAP) was given. 4 It is concluded that the pattern of absorption and plasma prednisolone levels depend on the formulation of the enteric coating. The bioavailability of the CAP based preparation is similar to that of plain prednisolone.", "contents": "The effect of food and tablet formulation on plasma prednisolone levels following administration of enteric-coated tablets. 1 Plasma prednisolone levels have been compared following the administration of enteric-coated prednisolone to fasted and non-fasted subjects. The effect on plasma levels of altering the formulation of the enteric-coating has also been studied. 2 The presence of food in the stomach at the time of administration does not affect the absorption of enteric-coated prednisolone tablets. 3 There was considerable inter-subject variation in plasma prednisolone levels after administration of shellac based enteric-coated tablets. However, plasma levels were more consistent when a preparation whose formulation was based upon cellulose acetate phthalate (CAP) was given. 4 It is concluded that the pattern of absorption and plasma prednisolone levels depend on the formulation of the enteric coating. The bioavailability of the CAP based preparation is similar to that of plain prednisolone."}
{"id": "PMID:475958", "title": "Tumour site and renal dysfunction as factors influencing leucopenia after chemotherapy for Burkitt's lymphoma.", "content": "Forty-four (44) patients with Burkitt's lymphoma received identical combination chemotherapy on the basis of body surface area. Patients with renal dysfunction, more common in those with abdominal tumours, were at significantly greater risk of developing severe leucopenia (less than 1000 cells/dl) than those with normal renal function (P less than 0.0001). Similar results were seen in a series of 8 patients with normal marrows treated with only i.v. cyclophosphamide and intrathecal methotrexate. Giving a lower initial dose of cyclophosphamide seemed to reduce the risk of severe leucopenia in 5 additional patients with evidence of renal dysfunction. The mechanism is postulated as delayed excretion of the active metabolites of cyclophosphamide. Adjustment of the chemotherapeutic dose should be considered when treating patients with renal dysfunction.", "contents": "Tumour site and renal dysfunction as factors influencing leucopenia after chemotherapy for Burkitt's lymphoma. Forty-four (44) patients with Burkitt's lymphoma received identical combination chemotherapy on the basis of body surface area. Patients with renal dysfunction, more common in those with abdominal tumours, were at significantly greater risk of developing severe leucopenia (less than 1000 cells/dl) than those with normal renal function (P less than 0.0001). Similar results were seen in a series of 8 patients with normal marrows treated with only i.v. cyclophosphamide and intrathecal methotrexate. Giving a lower initial dose of cyclophosphamide seemed to reduce the risk of severe leucopenia in 5 additional patients with evidence of renal dysfunction. The mechanism is postulated as delayed excretion of the active metabolites of cyclophosphamide. Adjustment of the chemotherapeutic dose should be considered when treating patients with renal dysfunction."}
{"id": "PMID:475960", "title": "Tissue distribution and tumour localization of 99m-technetium-labelled liposomes in cancer patients.", "content": "The possible use of liposomes (phospholipid vesicles) to direct cytotoxic drugs to tumours has led us to investigate the tissue localization of i.v. injected 99m-Tc-labelled liposomes in cancer patients. Twenty mg or 300 mg doses of liposomal lipid (7:2:1 molar ratio of phosphatidylcholine : cholesterol : phosphatidic acid) were used in a study of 13 patients with advanced cancer and one with polycythaemia rubra vera (PRV). In all cases except the patient with PRV the major site of uptake of the label was the liver and spleen. In the patient with PRV the liver uptake was greatly reduced and the major site of uptake was found in regions corresponding to marrow. With the exception of one patient with a primary hepatoma, there was no significant tumour uptake of the label.", "contents": "Tissue distribution and tumour localization of 99m-technetium-labelled liposomes in cancer patients. The possible use of liposomes (phospholipid vesicles) to direct cytotoxic drugs to tumours has led us to investigate the tissue localization of i.v. injected 99m-Tc-labelled liposomes in cancer patients. Twenty mg or 300 mg doses of liposomal lipid (7:2:1 molar ratio of phosphatidylcholine : cholesterol : phosphatidic acid) were used in a study of 13 patients with advanced cancer and one with polycythaemia rubra vera (PRV). In all cases except the patient with PRV the major site of uptake of the label was the liver and spleen. In the patient with PRV the liver uptake was greatly reduced and the major site of uptake was found in regions corresponding to marrow. With the exception of one patient with a primary hepatoma, there was no significant tumour uptake of the label."}
{"id": "PMID:475961", "title": "Results of treatment of female breast cancer in the Cambridge area 1960-71.", "content": "The results of treatment of female breast cancer in the Cambridge area during the period of 1960-71 are presented and compared with an earlier series treated in the period of 1947-50. It is estimated that about 29% of the patients treated in the later period are cured, in the sense that they have a life expectancy similar to that of the normal population, compared with just under 20% in the earlier period, but this improvement is mainly due to an increased proportion of Stage I cases in the latter period. The percentage cured is discussed in relation to the ratio of deaths to registrations in the East Anglian Region and it is suggested that under-registration of deaths from cancer of the breast may occur.", "contents": "Results of treatment of female breast cancer in the Cambridge area 1960-71. The results of treatment of female breast cancer in the Cambridge area during the period of 1960-71 are presented and compared with an earlier series treated in the period of 1947-50. It is estimated that about 29% of the patients treated in the later period are cured, in the sense that they have a life expectancy similar to that of the normal population, compared with just under 20% in the earlier period, but this improvement is mainly due to an increased proportion of Stage I cases in the latter period. The percentage cured is discussed in relation to the ratio of deaths to registrations in the East Anglian Region and it is suggested that under-registration of deaths from cancer of the breast may occur."}
{"id": "PMID:475962", "title": "Assays of drug sensitivity for cells from human tumours: in vitro and in vivo tests on a xenografted tumour.", "content": "A human tumour which grows as a xenograft in immune-suppressed mice and forms colonies in vitro has been used to test the correlation between 2 methods of exposure of human tumour cells to chemotherapeutic agents. In vivo exposure to drugs was achieved by injection of tumour-bearing mice with each of 8 cytotoxic agents. For the in vitro exposure, cell suspensions were incubated for 1 h with the same series of drugs. The survival of tumour clonogenic cells was assayed in vitro after either treatment or dose-response curves were obtained. The 8 drugs were ranked according to their in vivo effect at doses equitoxic to mice, and according to their in vitro effect at concentrations designed to approximate to levels of drugs in human plasma. The ranks for in vivo and in vitro exposure correlated well.", "contents": "Assays of drug sensitivity for cells from human tumours: in vitro and in vivo tests on a xenografted tumour. A human tumour which grows as a xenograft in immune-suppressed mice and forms colonies in vitro has been used to test the correlation between 2 methods of exposure of human tumour cells to chemotherapeutic agents. In vivo exposure to drugs was achieved by injection of tumour-bearing mice with each of 8 cytotoxic agents. For the in vitro exposure, cell suspensions were incubated for 1 h with the same series of drugs. The survival of tumour clonogenic cells was assayed in vitro after either treatment or dose-response curves were obtained. The 8 drugs were ranked according to their in vivo effect at doses equitoxic to mice, and according to their in vitro effect at concentrations designed to approximate to levels of drugs in human plasma. The ranks for in vivo and in vitro exposure correlated well."}
{"id": "PMID:475963", "title": "Inhibition of DNA synthesis by nitroheterocycles. I. Correlation with half-wave reduction potential.", "content": "Twenty-one nitroheterocycles, including metronidazole, misonidazole and AF-2, were tested for their ability to inhibit DNA synthesis in mouse L-929 cells growing in culture. All those tested inhibited the rate of incorporation of 3H-thymidine into L cells following drug treatment for 4 h under aerobic conditions. Only 4 drugs reached their limits of solubility before the uptake of 3H-thymidine was inhibited by 50% or more. For the remaining 17, the log of the concentration producing 50% inhibition of incorporation was directly correlated with the half-wave reduction potential of the compound.", "contents": "Inhibition of DNA synthesis by nitroheterocycles. I. Correlation with half-wave reduction potential. Twenty-one nitroheterocycles, including metronidazole, misonidazole and AF-2, were tested for their ability to inhibit DNA synthesis in mouse L-929 cells growing in culture. All those tested inhibited the rate of incorporation of 3H-thymidine into L cells following drug treatment for 4 h under aerobic conditions. Only 4 drugs reached their limits of solubility before the uptake of 3H-thymidine was inhibited by 50% or more. For the remaining 17, the log of the concentration producing 50% inhibition of incorporation was directly correlated with the half-wave reduction potential of the compound."}
{"id": "PMID:475964", "title": "Inhibition of DNA synthesis by nitroheterocycles. II. Mechanisms of cytotoxicity.", "content": "Nitroheterocycles have been shown to inhibit the incorporation of 3H-TdR by cultured L-929 cells, and the degree of inhibition is related to their electron-affinity. On the basis of their chemical reactivity and potential clinical utility, nitrofurazone, misonidazole and metronidazole were selected for more detailed studies of the mechanism of inhibition of DNA synthesis. Double-isotope labelling in conjunction with hydroxyapatite chromatography allowed the evaluation of drug effects on initiation of DNA replicons, DNA chain elongation and DNA damage (single-strand breaks), and their correlation with eventual cell viability. Partial inhibition of initiation of DNA synthesis generally preceded other measurable effects, and was not reversed by incubation in the absence of drug. In the absence of DNA strand breaks (at low drug doses or after a repair interval) the rate of elongation was similar in both treated and untreated cell populations. Measurable DNA damage (strand breaks) was predictive for cytotoxicity. At lower drug doses, or under aerobic conditions, DNA synthesis was not always associated with a decrease in plating efficiency (cytotoxicity) but was reflected in decreased colony size (growth rate) of the cells. Thus the aerobic \"toxicity\" previously reported for chronic exposure to these agents may be better described as a \"cytostatic\" effect. Under anaerobic conditions (where cell killing is much greater) inhibition of initiation plays a less important role, and the nitroheterocycles are metabolically reduced to intermediates which are truly cytotoxic.", "contents": "Inhibition of DNA synthesis by nitroheterocycles. II. Mechanisms of cytotoxicity. Nitroheterocycles have been shown to inhibit the incorporation of 3H-TdR by cultured L-929 cells, and the degree of inhibition is related to their electron-affinity. On the basis of their chemical reactivity and potential clinical utility, nitrofurazone, misonidazole and metronidazole were selected for more detailed studies of the mechanism of inhibition of DNA synthesis. Double-isotope labelling in conjunction with hydroxyapatite chromatography allowed the evaluation of drug effects on initiation of DNA replicons, DNA chain elongation and DNA damage (single-strand breaks), and their correlation with eventual cell viability. Partial inhibition of initiation of DNA synthesis generally preceded other measurable effects, and was not reversed by incubation in the absence of drug. In the absence of DNA strand breaks (at low drug doses or after a repair interval) the rate of elongation was similar in both treated and untreated cell populations. Measurable DNA damage (strand breaks) was predictive for cytotoxicity. At lower drug doses, or under aerobic conditions, DNA synthesis was not always associated with a decrease in plating efficiency (cytotoxicity) but was reflected in decreased colony size (growth rate) of the cells. Thus the aerobic \"toxicity\" previously reported for chronic exposure to these agents may be better described as a \"cytostatic\" effect. Under anaerobic conditions (where cell killing is much greater) inhibition of initiation plays a less important role, and the nitroheterocycles are metabolically reduced to intermediates which are truly cytotoxic."}
{"id": "PMID:475965", "title": "Racial differences in melanoma incidence.", "content": "The incidences of malignant melanoma recorded by 59 population-based cancer registries were investigated to determine the effects of racial and skin-colour differences. White populations exhibited a wide range of melanoma incidences and females commonly, though not invariably, had a higher incidence than males. Non-white populations experienced in general a much lower incidence of melanoma although there was some overlap of white and non-white rates. No predominant sex difference emerged among non-whites. Populations of African descent were found to have a higher incidence than those of Asiatic origin, but it was concluded that this was due largely to the high frequency of tumours among Africans on the sole of the foot. A clear negative correlation between degree of skin pigmentation and melanoma incidence emerged for the exposed body sites. These data provide strong support for the hypotheses that UV radiation is a major cause of malignant melanoma and that melanin pigmentation protects against it. Further research is required to elucidate the aetiology of melanoma of the sole of the foot.", "contents": "Racial differences in melanoma incidence. The incidences of malignant melanoma recorded by 59 population-based cancer registries were investigated to determine the effects of racial and skin-colour differences. White populations exhibited a wide range of melanoma incidences and females commonly, though not invariably, had a higher incidence than males. Non-white populations experienced in general a much lower incidence of melanoma although there was some overlap of white and non-white rates. No predominant sex difference emerged among non-whites. Populations of African descent were found to have a higher incidence than those of Asiatic origin, but it was concluded that this was due largely to the high frequency of tumours among Africans on the sole of the foot. A clear negative correlation between degree of skin pigmentation and melanoma incidence emerged for the exposed body sites. These data provide strong support for the hypotheses that UV radiation is a major cause of malignant melanoma and that melanin pigmentation protects against it. Further research is required to elucidate the aetiology of melanoma of the sole of the foot."}
{"id": "PMID:475967", "title": "Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases.", "content": "The modal DNA values of 1465 tumours, together with other factors of possible prognostic importance, were related to the survival of the patients, using regression models (Kay, 1977). For most tumour sites except the testis, the distributions of modal DNA values were bimodal, with peaks at the diploid level and in the triploid-tetraploid range. For all tumour sites except the cervix uteri, patients in the low (near-diploid) range showed better survival; the reverse was true for squamous-cell carcinoma of the cervix uteri. Other variables showed the following effects: for all sites except the testis, younger patients showed a better survival; for the cervix and corpus uteri, breast and ovary, increasing clinical stage was associated with poorer survival. Where evaluated, histological grade appeared to be associated with survival rate, the less well differentiated tumours having a worse prognosis, except for the breast, where the reverse correlation was noted. For carcinoma of the bladder, females and a poorer survival rate than males.", "contents": "Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases. The modal DNA values of 1465 tumours, together with other factors of possible prognostic importance, were related to the survival of the patients, using regression models (Kay, 1977). For most tumour sites except the testis, the distributions of modal DNA values were bimodal, with peaks at the diploid level and in the triploid-tetraploid range. For all tumour sites except the cervix uteri, patients in the low (near-diploid) range showed better survival; the reverse was true for squamous-cell carcinoma of the cervix uteri. Other variables showed the following effects: for all sites except the testis, younger patients showed a better survival; for the cervix and corpus uteri, breast and ovary, increasing clinical stage was associated with poorer survival. Where evaluated, histological grade appeared to be associated with survival rate, the less well differentiated tumours having a worse prognosis, except for the breast, where the reverse correlation was noted. For carcinoma of the bladder, females and a poorer survival rate than males."}
{"id": "PMID:475966", "title": "Giant-cell tumour of bone: cytological studies.", "content": "Cell-surface markers were investigated in 7 patients with giant-cell tumours and 30 patients with other tumours as controls. 25--55% of mononuclear cells in giant-cell tumours showed immunoglobulin-mediated phagocytosis. These phagocytic cells showed rapid adherence, trypsin resistance and potent nonspecific esterase activity. Thus, giant-cell tumours contained considerable numbers of macrophages with typical characteristics and functions. Macrophages did not proliferate in cultures of giant-cell tumours, whereas the non-adherent cells did. Further, established cell lines from these tumours consisted of spindle-shaped cells without surface markers or the ability to phagocytose or display nonspecific esterase activity. We consider that macrophages, which may be precursors of giant cells in giant-cell tumours, are non-malignant cells of host origin rather than tumour cells acquiring some properties of macrophages. We found that macrophages were more abundant in giant-cell tumours than in other tumours of mesenchymal origin, but any effect of their presence on the clinical behaviour and prognosis of the tumour remains highly speculative.", "contents": "Giant-cell tumour of bone: cytological studies. Cell-surface markers were investigated in 7 patients with giant-cell tumours and 30 patients with other tumours as controls. 25--55% of mononuclear cells in giant-cell tumours showed immunoglobulin-mediated phagocytosis. These phagocytic cells showed rapid adherence, trypsin resistance and potent nonspecific esterase activity. Thus, giant-cell tumours contained considerable numbers of macrophages with typical characteristics and functions. Macrophages did not proliferate in cultures of giant-cell tumours, whereas the non-adherent cells did. Further, established cell lines from these tumours consisted of spindle-shaped cells without surface markers or the ability to phagocytose or display nonspecific esterase activity. We consider that macrophages, which may be precursors of giant cells in giant-cell tumours, are non-malignant cells of host origin rather than tumour cells acquiring some properties of macrophages. We found that macrophages were more abundant in giant-cell tumours than in other tumours of mesenchymal origin, but any effect of their presence on the clinical behaviour and prognosis of the tumour remains highly speculative."}
{"id": "PMID:475968", "title": "Inactivation by the mitotic inhibitor NY 3170 of human cells in vitro.", "content": "Inactivation of NHIK 3025 cells ny the mitotic inhibitor NY 3170 (1-propargyl-5-chloropyrimidin-2-one) was measured as loss of colony-forming ability. NY 3170 at a concentration of 0.15 nM allowed no formation of colonies after 12 days of continuous exposure to the drug. Metaphase arrest after treatment with NY 3170 was reversible if the drug was removed immediately after the onset of the arrest. When the cells were kept in mitosis by the presence of NY 3170, inactivation was complete after 8h incubation of mitotic cells with 0.4 nM NY 3170. Using synchronized cell populations, it was shown that mitosis is by far the most sensitive stage of the cell cycle to inactivation by NY 3170. This leads to the suggestion that there is a connection between the inactivating and the metaphase-arresting effect of this drug. The age response curves show that after mitosis the stages in order of decreasing sensitivity to NY3170 are: G2, late S, early S and G1. This is a similar age response to that reported for proliferating cells treated with bleomycin, whereas the mitotic inhibitors vincristine and vinblastine have shown qhite different age response curves.", "contents": "Inactivation by the mitotic inhibitor NY 3170 of human cells in vitro. Inactivation of NHIK 3025 cells ny the mitotic inhibitor NY 3170 (1-propargyl-5-chloropyrimidin-2-one) was measured as loss of colony-forming ability. NY 3170 at a concentration of 0.15 nM allowed no formation of colonies after 12 days of continuous exposure to the drug. Metaphase arrest after treatment with NY 3170 was reversible if the drug was removed immediately after the onset of the arrest. When the cells were kept in mitosis by the presence of NY 3170, inactivation was complete after 8h incubation of mitotic cells with 0.4 nM NY 3170. Using synchronized cell populations, it was shown that mitosis is by far the most sensitive stage of the cell cycle to inactivation by NY 3170. This leads to the suggestion that there is a connection between the inactivating and the metaphase-arresting effect of this drug. The age response curves show that after mitosis the stages in order of decreasing sensitivity to NY3170 are: G2, late S, early S and G1. This is a similar age response to that reported for proliferating cells treated with bleomycin, whereas the mitotic inhibitors vincristine and vinblastine have shown qhite different age response curves."}
{"id": "PMID:475969", "title": "Antibody-dependent cellular cytotoxicity to human colon-tumour cells. I. Lack of tumour specificity in a population study.", "content": "The humoral and cellular components of the antibody-dependent cellular cytotoxicity (ADCC) against allogeneic human colonic tumour cell lines were evaluated. The 2 colon cell lines used in this study (HT-29 and ACC-20) were found by immunofluorescence to have carcinoembryonic antigen (CEA) on their surface, and to become sensitive to the lytic effect of unstimulated lymphocytes after coating with heterologous anti-CEA. This reaction was used to evaluate the ADCC activity of mononuclear cells from the peripheral blood of patients with gastrointestinal cancer (mostly local extensive colo-rectal). Remarkable variability was found in the lytic capability (2-50% specific lysis) of both cancer and non-cancer mononuclear cells, with no significant difference between them. Sera from 127 cancer patients and 91 non-cancer patients were tested, using the reaction with heterologous anti-CEA as positive control and as a reference point. In 46 cases (21%) the sera were reactive in this system, and 43 of them were of Blood Group O. However, there was no difference between the cancer patients and the normal controls. The antigenic determinant involved in this reaction is not the Blood Group A specificity but, most probably, a polypeptide common to CEA and A (as shown in the following publication). In addition, trials for the elimination of the non-tumour-specific reaction, by absorption or inhibition, failed to disclose a tumour-specific one. The value of the ADCC assay in monitoring human tumour immunity, and possible ways of eliminating reactivity to normal antigens in this system, are discussed in the light of these findings.", "contents": "Antibody-dependent cellular cytotoxicity to human colon-tumour cells. I. Lack of tumour specificity in a population study. The humoral and cellular components of the antibody-dependent cellular cytotoxicity (ADCC) against allogeneic human colonic tumour cell lines were evaluated. The 2 colon cell lines used in this study (HT-29 and ACC-20) were found by immunofluorescence to have carcinoembryonic antigen (CEA) on their surface, and to become sensitive to the lytic effect of unstimulated lymphocytes after coating with heterologous anti-CEA. This reaction was used to evaluate the ADCC activity of mononuclear cells from the peripheral blood of patients with gastrointestinal cancer (mostly local extensive colo-rectal). Remarkable variability was found in the lytic capability (2-50% specific lysis) of both cancer and non-cancer mononuclear cells, with no significant difference between them. Sera from 127 cancer patients and 91 non-cancer patients were tested, using the reaction with heterologous anti-CEA as positive control and as a reference point. In 46 cases (21%) the sera were reactive in this system, and 43 of them were of Blood Group O. However, there was no difference between the cancer patients and the normal controls. The antigenic determinant involved in this reaction is not the Blood Group A specificity but, most probably, a polypeptide common to CEA and A (as shown in the following publication). In addition, trials for the elimination of the non-tumour-specific reaction, by absorption or inhibition, failed to disclose a tumour-specific one. The value of the ADCC assay in monitoring human tumour immunity, and possible ways of eliminating reactivity to normal antigens in this system, are discussed in the light of these findings."}
{"id": "PMID:475970", "title": "Tissue distribution and biochemical properties of an interspecific tumour-associated gamma foetal antigen.", "content": "A late-gestation neonatal antigen (gamma foetal antigen; gamma-FA) immunologically and biochemically unrelated to murine alpha-foetoprotein, was identified in several spontaneous and carcinogen-induced sarcomas and hepatic carcinomas of the mouse and rat. An approximate mol. wt of 35,000 for gamma-FA from both foetus and tumour was obtained by molecular-sieve chromatography and sucrose-gradient centrifugation. Radial immunodiffusion analyses of organ extracts indicated that gamma-FA could be found in several neonatal tissues, the highest concentration occurring in the spleen. In the 2-month-old mouse, only splenic tissue contained gamma-FA and at much lower levels than in the organ of the newborn mouse.", "contents": "Tissue distribution and biochemical properties of an interspecific tumour-associated gamma foetal antigen. A late-gestation neonatal antigen (gamma foetal antigen; gamma-FA) immunologically and biochemically unrelated to murine alpha-foetoprotein, was identified in several spontaneous and carcinogen-induced sarcomas and hepatic carcinomas of the mouse and rat. An approximate mol. wt of 35,000 for gamma-FA from both foetus and tumour was obtained by molecular-sieve chromatography and sucrose-gradient centrifugation. Radial immunodiffusion analyses of organ extracts indicated that gamma-FA could be found in several neonatal tissues, the highest concentration occurring in the spleen. In the 2-month-old mouse, only splenic tissue contained gamma-FA and at much lower levels than in the organ of the newborn mouse."}
{"id": "PMID:475971", "title": "Effects of discontinuation of chronic feeding of diethylnitrosamine on the development of hepatomas in adult rats.", "content": "Diethylnitrosamine (DENA) at 10 mg/kg was fed to adult rats either continuously or for periods ranging from 1 to 10 weeks. Survival correlated inversely with the duration of carcinogen feeding. Less than 4 weeks of DENA feeding produced only preneoplastic foci that persisted indefinitely; 4 weeks were found to be necessary for the transformation of preneoplastic lesions into liver cancers; after 6 weeks, the incidence of hepatomas was 100%. The process of liver cancerization appeared to be identical whether DENA was fed for 8 weeks or continuously up to the time of death. These results are discussed in the light of the evolution of the homoeostatic control of liver-cell division during DENA feeding, in order to distinguish the different successive roles played by the carcinogen.", "contents": "Effects of discontinuation of chronic feeding of diethylnitrosamine on the development of hepatomas in adult rats. Diethylnitrosamine (DENA) at 10 mg/kg was fed to adult rats either continuously or for periods ranging from 1 to 10 weeks. Survival correlated inversely with the duration of carcinogen feeding. Less than 4 weeks of DENA feeding produced only preneoplastic foci that persisted indefinitely; 4 weeks were found to be necessary for the transformation of preneoplastic lesions into liver cancers; after 6 weeks, the incidence of hepatomas was 100%. The process of liver cancerization appeared to be identical whether DENA was fed for 8 weeks or continuously up to the time of death. These results are discussed in the light of the evolution of the homoeostatic control of liver-cell division during DENA feeding, in order to distinguish the different successive roles played by the carcinogen."}
{"id": "PMID:475972", "title": "Release from the Crabtree effect by hypoxic cell radiosensitizers.", "content": "The Crabtree effect can be observed when the O2 consumption of tumour cells or of mammalian cells grown in culture is measured in physiological medium containing glucose. The effect of 2 hypoxic cell radiosensitizers, misonidazole and NDPP, on the O2 consumption of Ehrlich ascites tumour cells was compared in media with and without glucose. A stimulatory effect on O2 consumption was found for 5--20mM misonidazole as well as for 0.5mM NDPP, both in media containing 10(-2)M glucose. Thus glucose induced a Crabtree effect in Ehrlich tumour cells, expressed as 38--45% inhibition of O2 consumption relative to that in the same medium without glucose. The stimulatory effect of misonidazole and NDPP on O2 utilization in medium with glucose undoubtedly appeared as a release from the Crabtree effect.", "contents": "Release from the Crabtree effect by hypoxic cell radiosensitizers. The Crabtree effect can be observed when the O2 consumption of tumour cells or of mammalian cells grown in culture is measured in physiological medium containing glucose. The effect of 2 hypoxic cell radiosensitizers, misonidazole and NDPP, on the O2 consumption of Ehrlich ascites tumour cells was compared in media with and without glucose. A stimulatory effect on O2 consumption was found for 5--20mM misonidazole as well as for 0.5mM NDPP, both in media containing 10(-2)M glucose. Thus glucose induced a Crabtree effect in Ehrlich tumour cells, expressed as 38--45% inhibition of O2 consumption relative to that in the same medium without glucose. The stimulatory effect of misonidazole and NDPP on O2 utilization in medium with glucose undoubtedly appeared as a release from the Crabtree effect."}
{"id": "PMID:475973", "title": "Effect of human cord and postoperative serum on experimental inflammation in the rat.", "content": "Human cord and postoperative serum depressed the oedema provoked by mediators of the inflammatory reaction such as bradykinin, histamine, serotonin and prostaglandin E2 and also the experimental inflammation caused by carrageenin. Normal human and pregnancy serum did not have such an effect. In two cases of open neural tube defect, one of anencephaly and another of spina bifida, human amniotic fluid also had a strongly depressing effect on the experimental oedema provoked by serotonin. Human amniotic fluid from normal pregnancies did not inhibit this experimental inflammation. A protein-fraction of mol. wt 30,000--100,000 has been isolated from the inhibiting sera and shows the anti-inflammatory activity to be dose-related towards all the oedema-provoking substances used. Immunological studies showed that the inhibiting factor could be a protein in the pre-albuminic region, while alpha-foetoprotein did not appear to be responsible for the anti-inflammatory activity. Our conclusion is that human serum contains a protein of foetal origin with an acute-phase character and strong anti-inflammatory activities analogous to rat alpha 2-macrofoetoprotein.", "contents": "Effect of human cord and postoperative serum on experimental inflammation in the rat. Human cord and postoperative serum depressed the oedema provoked by mediators of the inflammatory reaction such as bradykinin, histamine, serotonin and prostaglandin E2 and also the experimental inflammation caused by carrageenin. Normal human and pregnancy serum did not have such an effect. In two cases of open neural tube defect, one of anencephaly and another of spina bifida, human amniotic fluid also had a strongly depressing effect on the experimental oedema provoked by serotonin. Human amniotic fluid from normal pregnancies did not inhibit this experimental inflammation. A protein-fraction of mol. wt 30,000--100,000 has been isolated from the inhibiting sera and shows the anti-inflammatory activity to be dose-related towards all the oedema-provoking substances used. Immunological studies showed that the inhibiting factor could be a protein in the pre-albuminic region, while alpha-foetoprotein did not appear to be responsible for the anti-inflammatory activity. Our conclusion is that human serum contains a protein of foetal origin with an acute-phase character and strong anti-inflammatory activities analogous to rat alpha 2-macrofoetoprotein."}
{"id": "PMID:475976", "title": "The effects of perhexiline maleate (Pexid) and alcohol on rat liver.", "content": "Perhexiline maleate is an anti-anginal drug believed to cause liver damage. The possibility that this effect could be potentiated by alcohol has been investigated. Rats fed both the drug and alcohol did not show more severe liver damage than those fed alcohol alone. Livers from rats fed perhexiline alone did not differ from controls. There is no evidence from this study that alcohol potentiates the effect of perhexiline in rat liver. These results may have a bearing in perhexiline-induced liver damage in humans, but clinical studies are required.", "contents": "The effects of perhexiline maleate (Pexid) and alcohol on rat liver. Perhexiline maleate is an anti-anginal drug believed to cause liver damage. The possibility that this effect could be potentiated by alcohol has been investigated. Rats fed both the drug and alcohol did not show more severe liver damage than those fed alcohol alone. Livers from rats fed perhexiline alone did not differ from controls. There is no evidence from this study that alcohol potentiates the effect of perhexiline in rat liver. These results may have a bearing in perhexiline-induced liver damage in humans, but clinical studies are required."}
{"id": "PMID:475974", "title": "An ultrastructural study of peritoneal mononuclear phagocytes from Corynebacterium parvum-injected mice.", "content": "Peritoneal mononuclear phagocytes harvested from mice 24 h after i.p. injection of C. parvum displayed hypertrophy of the Golgi and smooth endoplasmic reticulum complex with attendant increase in lysosome production. The ingested bacilli were identified within phagolysosomes, within which large myelin figures accumulated. In addition to heterophagic vacuoles, autophagosomes and lipid droplets were observed. These latter two inclusions may reflect a direct or indirect cytopathic effect of C. parvum on the phagocytes.", "contents": "An ultrastructural study of peritoneal mononuclear phagocytes from Corynebacterium parvum-injected mice. Peritoneal mononuclear phagocytes harvested from mice 24 h after i.p. injection of C. parvum displayed hypertrophy of the Golgi and smooth endoplasmic reticulum complex with attendant increase in lysosome production. The ingested bacilli were identified within phagolysosomes, within which large myelin figures accumulated. In addition to heterophagic vacuoles, autophagosomes and lipid droplets were observed. These latter two inclusions may reflect a direct or indirect cytopathic effect of C. parvum on the phagocytes."}
{"id": "PMID:475977", "title": "Effects of non-haemorrhagic injury on the cardiovascular response to tilting in the rat.", "content": "In the unanaesthetized uninjured rat a head-up tilt elicited a pressor response, the magnitude of which was directly related to the angle of tilt. The pressor response was reduced by a 4 h period of bilateral hind-limb ischaemia and by a non-fatal scald, although the slope of the regression line relating percentage rise in mean arterial blood pressure to the angle of tilt was unaltered. The degree of impairment of the response was directly related to the severity of the injury. In the uninjured rat there was a sigmoid relationship between the angle of tilt and the mean heart rate during the tilt. No relationship could be demonstrated between angle of tilt and heart rate in the injured rats.", "contents": "Effects of non-haemorrhagic injury on the cardiovascular response to tilting in the rat. In the unanaesthetized uninjured rat a head-up tilt elicited a pressor response, the magnitude of which was directly related to the angle of tilt. The pressor response was reduced by a 4 h period of bilateral hind-limb ischaemia and by a non-fatal scald, although the slope of the regression line relating percentage rise in mean arterial blood pressure to the angle of tilt was unaltered. The degree of impairment of the response was directly related to the severity of the injury. In the uninjured rat there was a sigmoid relationship between the angle of tilt and the mean heart rate during the tilt. No relationship could be demonstrated between angle of tilt and heart rate in the injured rats."}
{"id": "PMID:475975", "title": "Inhibitory effect of pregnancy on the migration of the inflammatory cells: a quantitative histological study.", "content": "The migration of inflammatory cells and fibroblasts into dacron mesh tissue (Mersilene), immersed in saline or in a solution containing live BCG and implanted under the skin of virgin and pregnant female guinea-pigs, was investigated. Cell counts have shown that in pregnant females significant reduction (about 50%) of migration of the inflammatory cells occurred in both saline and live-BCG-immersed implants. The effect was maximal (-81%) on macrophage level in implants immersed in saline, whereas the action was prominent on neutrophils level (-66%) in implants containing live BCG. In the case of implants devoid of live BCG, the inhibitory effect of pregnancy on migration of the inflammatory cells and fibroblasts seemed not to be directly related to any immunological process. This experiment suggests that inhibition could be mediated via an undefined factor interacting with cell membranes to diminish their functional activities.", "contents": "Inhibitory effect of pregnancy on the migration of the inflammatory cells: a quantitative histological study. The migration of inflammatory cells and fibroblasts into dacron mesh tissue (Mersilene), immersed in saline or in a solution containing live BCG and implanted under the skin of virgin and pregnant female guinea-pigs, was investigated. Cell counts have shown that in pregnant females significant reduction (about 50%) of migration of the inflammatory cells occurred in both saline and live-BCG-immersed implants. The effect was maximal (-81%) on macrophage level in implants immersed in saline, whereas the action was prominent on neutrophils level (-66%) in implants containing live BCG. In the case of implants devoid of live BCG, the inhibitory effect of pregnancy on migration of the inflammatory cells and fibroblasts seemed not to be directly related to any immunological process. This experiment suggests that inhibition could be mediated via an undefined factor interacting with cell membranes to diminish their functional activities."}
{"id": "PMID:475979", "title": "A morphological assessment of macrophages attaching to subcutaneously implanted coverslips in dogs with chronic lymphoedema.", "content": "In an experimental model of chronic lymphoedema in the dog leg it has been shown that the presence of lymphoedema was associated with reduced numbers of macrophages found on subcutaneously implanted coverslips. Total numbers of mononuclear cells were not, however, significantly affected. When expressed as a percentage of the total mononuclear cells attached, the lymphoedematous limbs always had only half those of the corresponding normal limbs. Similarly, the percentage of macrophages with two or more pseudopods on the coverslips in the lymphoedematous limbs was only 1/3 of that for the same morphological criteria in normal limbs. However, when the macrophages with vacuoles were expressed as a percentage of the total macrophages, there was always a higher percentage in the lymphoedematous limbs compared with the corresponding normal ones.", "contents": "A morphological assessment of macrophages attaching to subcutaneously implanted coverslips in dogs with chronic lymphoedema. In an experimental model of chronic lymphoedema in the dog leg it has been shown that the presence of lymphoedema was associated with reduced numbers of macrophages found on subcutaneously implanted coverslips. Total numbers of mononuclear cells were not, however, significantly affected. When expressed as a percentage of the total mononuclear cells attached, the lymphoedematous limbs always had only half those of the corresponding normal limbs. Similarly, the percentage of macrophages with two or more pseudopods on the coverslips in the lymphoedematous limbs was only 1/3 of that for the same morphological criteria in normal limbs. However, when the macrophages with vacuoles were expressed as a percentage of the total macrophages, there was always a higher percentage in the lymphoedematous limbs compared with the corresponding normal ones."}
{"id": "PMID:475978", "title": "In vitro prediction of the pathogenicity of mineral dusts.", "content": "Eight mineral dusts, of which 7 were fibrous, were tested in 3 in vitro systems. The dusts had previously been tested for their ability to produce mesotheliomata in rats. The in vivo and in vitro biological activities of these dusts are compared with the number and size of fibres they contain. It is concluded that all the in vitro systems would predict which dusts are pathogenic in vivo, and that both in vivo and in vitro biological activities are determined by the number of long, fine fibres.", "contents": "In vitro prediction of the pathogenicity of mineral dusts. Eight mineral dusts, of which 7 were fibrous, were tested in 3 in vitro systems. The dusts had previously been tested for their ability to produce mesotheliomata in rats. The in vivo and in vitro biological activities of these dusts are compared with the number and size of fibres they contain. It is concluded that all the in vitro systems would predict which dusts are pathogenic in vivo, and that both in vivo and in vitro biological activities are determined by the number of long, fine fibres."}
{"id": "PMID:475980", "title": "The effect of a calcium antagonist (D600) on isoprenaline-induced myocardial necrosis in the rat.", "content": "The effect of inhibiting isoprenaline-induced intracellular calcium accumulation on the degree of damage produced in the rat myocardium by this amine has been investigated by simultaneously dosing rats with the calcium antagonistic drug D600. The degree of myocardial necrosis produced in animals given isoprenaline alone and those given D600 was measured by the use of a standard point counting method to show absence of formazan from dead muscle fibres in sections treated to demonstrate succinic dehydrogenase. The use of the calcium antagonistic drug D600 considerably reduced the degree of myocardial damage produced by a standard dose of isoprenaline bitartrate. This was associated with a decrease in the isoprenaline-induced cellular calcium uptake. The results are discussed in relation to the possible protection conferred by lowering of the calcium influx at the cell membrane and the maintenance of the cells' high-energy phosphates at a level which permits \"normal\" cell function.", "contents": "The effect of a calcium antagonist (D600) on isoprenaline-induced myocardial necrosis in the rat. The effect of inhibiting isoprenaline-induced intracellular calcium accumulation on the degree of damage produced in the rat myocardium by this amine has been investigated by simultaneously dosing rats with the calcium antagonistic drug D600. The degree of myocardial necrosis produced in animals given isoprenaline alone and those given D600 was measured by the use of a standard point counting method to show absence of formazan from dead muscle fibres in sections treated to demonstrate succinic dehydrogenase. The use of the calcium antagonistic drug D600 considerably reduced the degree of myocardial damage produced by a standard dose of isoprenaline bitartrate. This was associated with a decrease in the isoprenaline-induced cellular calcium uptake. The results are discussed in relation to the possible protection conferred by lowering of the calcium influx at the cell membrane and the maintenance of the cells' high-energy phosphates at a level which permits \"normal\" cell function."}
{"id": "PMID:475983", "title": "The effect of substrate on the uptake of oxygen and sulphate ions by heat-damaged skin.", "content": "Respiration and sulphate uptake have been measured in guinea-pig skin subjected to heat damage and maintained on a medium containing sodium succinate. Succinate was found to have a protective effect against loss of oxygen uptake, a higher temperature being required for 50% effect than that reported with glucose as substrate. The protective effect did not extend to sulphate incorporation, suggesting that this process is itself heat-labile and that its failure with heat damage is not directly caused by diminution of the energy supply.", "contents": "The effect of substrate on the uptake of oxygen and sulphate ions by heat-damaged skin. Respiration and sulphate uptake have been measured in guinea-pig skin subjected to heat damage and maintained on a medium containing sodium succinate. Succinate was found to have a protective effect against loss of oxygen uptake, a higher temperature being required for 50% effect than that reported with glucose as substrate. The protective effect did not extend to sulphate incorporation, suggesting that this process is itself heat-labile and that its failure with heat damage is not directly caused by diminution of the energy supply."}
{"id": "PMID:475984", "title": "Leukocyte function in granuloma annulare.", "content": "Leukocytes from patients with granuloma annulare were studied in vitro in order to detect functional abnormalities. Random migration and chemotaxis of both polymorphonuclear leukocytes and monocytes were similar in patients and controls. Preincubation of normal cells with patients' plasma did not enhance or inhibit locomotion. Phagocytosis and oxidative burst as reflected by chemiluminescence were also normal. Peripheral blood lymphocytes incorporated uridine normally with and without stimulation by the non-specific mitogen phytohaemagglutinin. These studies suggest that peripheral blood leukocyte function in patients with granuloma annulare is normal, but do not exclude a functional abnormality in tissue.", "contents": "Leukocyte function in granuloma annulare. Leukocytes from patients with granuloma annulare were studied in vitro in order to detect functional abnormalities. Random migration and chemotaxis of both polymorphonuclear leukocytes and monocytes were similar in patients and controls. Preincubation of normal cells with patients' plasma did not enhance or inhibit locomotion. Phagocytosis and oxidative burst as reflected by chemiluminescence were also normal. Peripheral blood lymphocytes incorporated uridine normally with and without stimulation by the non-specific mitogen phytohaemagglutinin. These studies suggest that peripheral blood leukocyte function in patients with granuloma annulare is normal, but do not exclude a functional abnormality in tissue."}
{"id": "PMID:475985", "title": "Comparison of immunoglobulin and complement deposition in multiple biopsies from the uninvolved skin in dermatitis herpetiformis.", "content": "The distribution of immunoglobulins and C3 component of complement (C3) in the skin of twenty-four patients with dermatitis herpetiformis was studied. Skin biopsies were taken from three sites, the extensor surface of the elbow, the flexor surface of the forearm and from the thigh. Twenty-two (90%) patients showed variation in deposits of immunoglobulins and C3 at the three sites. IgA was present in all patients, but differed in quantities deposited at the three sites in nineteen (80%) patients. Similar variation between sites was observed with IgG, IgM and C3. There was variation within three biopsies, IgA being absent from some sections and present in others. Three patients had a continuous pattern of IgA deposition. One had both continuous and papillary deposits within a single section. There was no difference in the incidence or quantity of immunoglobulin and C3 between the elbow, forearm and thigh. There was observed to be a diminution in quantity of IgA and incidence of C3 in patients taking a gluten-free diet. Deposition of IgA, IgM, IgG and C3 is not uniform throughout the skin and conclusions drawn from the quantity of immunoglobulin and C3 in a single biopsy may not be reliable.", "contents": "Comparison of immunoglobulin and complement deposition in multiple biopsies from the uninvolved skin in dermatitis herpetiformis. The distribution of immunoglobulins and C3 component of complement (C3) in the skin of twenty-four patients with dermatitis herpetiformis was studied. Skin biopsies were taken from three sites, the extensor surface of the elbow, the flexor surface of the forearm and from the thigh. Twenty-two (90%) patients showed variation in deposits of immunoglobulins and C3 at the three sites. IgA was present in all patients, but differed in quantities deposited at the three sites in nineteen (80%) patients. Similar variation between sites was observed with IgG, IgM and C3. There was variation within three biopsies, IgA being absent from some sections and present in others. Three patients had a continuous pattern of IgA deposition. One had both continuous and papillary deposits within a single section. There was no difference in the incidence or quantity of immunoglobulin and C3 between the elbow, forearm and thigh. There was observed to be a diminution in quantity of IgA and incidence of C3 in patients taking a gluten-free diet. Deposition of IgA, IgM, IgG and C3 is not uniform throughout the skin and conclusions drawn from the quantity of immunoglobulin and C3 in a single biopsy may not be reliable."}
{"id": "PMID:475986", "title": "Melanotic freckle of Hutchinson: treatment of macular and nodular phases with cryotherapy.", "content": "Fourteen cases of Hutchinson's lentigo maligna and eight further cases with nodular invasive areas in whom liquid nitrogen spray has been curative are reported. We suggest that in view of its simplicity and efficacy, cryotherapy is the treatment of choice for the macular phase and possibly also for the nodular invasive stage.", "contents": "Melanotic freckle of Hutchinson: treatment of macular and nodular phases with cryotherapy. Fourteen cases of Hutchinson's lentigo maligna and eight further cases with nodular invasive areas in whom liquid nitrogen spray has been curative are reported. We suggest that in view of its simplicity and efficacy, cryotherapy is the treatment of choice for the macular phase and possibly also for the nodular invasive stage."}
{"id": "PMID:475987", "title": "Treatment of cold urticaria.", "content": "In a single patient with severe essential acquired cold urticaria, the following drug regimes were assessed: diphenhydramine, cyproheptadine, hydroxyzine, terbutaline, cimetidine, hydroxyzine plus cimetidine, and hydroxyzine plus terbutaline. Each regime showed favourable results, but a significance level of P = 0.01 was reached only for (a) hydroxyzine plus cimetidine in the suppression of erythema, and (b) the histamine H1 antagonists in the suppression of wealing.", "contents": "Treatment of cold urticaria. In a single patient with severe essential acquired cold urticaria, the following drug regimes were assessed: diphenhydramine, cyproheptadine, hydroxyzine, terbutaline, cimetidine, hydroxyzine plus cimetidine, and hydroxyzine plus terbutaline. Each regime showed favourable results, but a significance level of P = 0.01 was reached only for (a) hydroxyzine plus cimetidine in the suppression of erythema, and (b) the histamine H1 antagonists in the suppression of wealing."}
{"id": "PMID:475988", "title": "The effect of H1 and H2 histamine antagonists on symptomatic dermographism.", "content": "In ten patients suffering from symptomatic dermographism the combined administration of chlorpheniramine + cimetidine produced a greater reduction in the weal and flare response provoked by a standardized scratch than the administration of chlorpheniramine alone. There was a statistically significant improvement in the overall assessment of the patient's skin condition with the combined administration of chlorpheniramine + cimetidine. Chlorpheniramine given alone produced no significant benefit whilst cimetidine alone produced a marked exacerbation in itching in nearly half the patients who initially entered the study and was sufficient to require withdrawal.", "contents": "The effect of H1 and H2 histamine antagonists on symptomatic dermographism. In ten patients suffering from symptomatic dermographism the combined administration of chlorpheniramine + cimetidine produced a greater reduction in the weal and flare response provoked by a standardized scratch than the administration of chlorpheniramine alone. There was a statistically significant improvement in the overall assessment of the patient's skin condition with the combined administration of chlorpheniramine + cimetidine. Chlorpheniramine given alone produced no significant benefit whilst cimetidine alone produced a marked exacerbation in itching in nearly half the patients who initially entered the study and was sufficient to require withdrawal."}
{"id": "PMID:475989", "title": "Tuberculoid cutaneous infection due to a niacin-positive Mycobacterium chelonei.", "content": "A case of cutaneous infection due to a niacin-positive Mycobacterium chelonei (previously called M. abscessus) on the dorsum of the hand of a professional cotton-classifier is reported from Hong Kong. The infection was probably directly acquired from handling contaminated raw cotton. The patient was successfully treated with combined anti-tuberculous drugs over a period of 1 year. Histologically, the skin lesion showed tuberculoid granulomas, in addition to abscesses.", "contents": "Tuberculoid cutaneous infection due to a niacin-positive Mycobacterium chelonei. A case of cutaneous infection due to a niacin-positive Mycobacterium chelonei (previously called M. abscessus) on the dorsum of the hand of a professional cotton-classifier is reported from Hong Kong. The infection was probably directly acquired from handling contaminated raw cotton. The patient was successfully treated with combined anti-tuberculous drugs over a period of 1 year. Histologically, the skin lesion showed tuberculoid granulomas, in addition to abscesses."}
{"id": "PMID:475990", "title": "Mycobacterium avium infection of the skin resembling lepromatous leprosy.", "content": "A patient on systemic steroid therapy developed a cutaneous papule while histologically resembled lepromatous leprosy. Cultures of this lesion grew Mycobacterium avium. Since there was no evidence of disseminated infection the lesion was excised and the patient continues to do well.", "contents": "Mycobacterium avium infection of the skin resembling lepromatous leprosy. A patient on systemic steroid therapy developed a cutaneous papule while histologically resembled lepromatous leprosy. Cultures of this lesion grew Mycobacterium avium. Since there was no evidence of disseminated infection the lesion was excised and the patient continues to do well."}
{"id": "PMID:475991", "title": "A sporotrichoid-like Mycobacterium kansasii infection of the skin treated with minocycline hydrochloride.", "content": "A sporotrichoid-like Mycobacterium kansasii infection of the skin is reported. This is the fifth reported case in the English literature of dermatological manifestations of a M. kansasii infection and the first reported case of a response to minocycline hydrochloride therapy.", "contents": "A sporotrichoid-like Mycobacterium kansasii infection of the skin treated with minocycline hydrochloride. A sporotrichoid-like Mycobacterium kansasii infection of the skin is reported. This is the fifth reported case in the English literature of dermatological manifestations of a M. kansasii infection and the first reported case of a response to minocycline hydrochloride therapy."}
{"id": "PMID:475992", "title": "Neonatal lupus erythematosus progressing into systemic lupus erythematosus. A 15 year follow-up.", "content": "An infant with neonatal lupus erythematosus developed systemic lupus erythematosus at the age of 13. The clinical distinction between neonatal lupus erythematosus and familial lupus erythematosus may not be as clear cut as previously reported.", "contents": "Neonatal lupus erythematosus progressing into systemic lupus erythematosus. A 15 year follow-up. An infant with neonatal lupus erythematosus developed systemic lupus erythematosus at the age of 13. The clinical distinction between neonatal lupus erythematosus and familial lupus erythematosus may not be as clear cut as previously reported."}
{"id": "PMID:475993", "title": "Systemic lupus erythematosus in pregnancy treated with plasmapheresis.", "content": "A young woman developed severe systemic lupus erythematosus (SLE) at the beginning of her second pregnancy: her disease was extremely difficult to control and the baby died unexpectedly when 8 weeks old. She became very ill at the same time in her third pregnancy; plasmapheresis appeared to make her management easier, and the 7-month-old baby is doing well.", "contents": "Systemic lupus erythematosus in pregnancy treated with plasmapheresis. A young woman developed severe systemic lupus erythematosus (SLE) at the beginning of her second pregnancy: her disease was extremely difficult to control and the baby died unexpectedly when 8 weeks old. She became very ill at the same time in her third pregnancy; plasmapheresis appeared to make her management easier, and the 7-month-old baby is doing well."}
{"id": "PMID:475994", "title": "Systemic sclerosis with subcutaneous nodules.", "content": "A case of systemic sclerosis with subcutaneous nodules is described. The nodules consisted of fibrinoid degeneration with surrounding fibrosis, but lacked the typical histiocytic palisade of the rheumatoid nodule. The patient had neither coexistent rheumatoid arthritis nor circulating rheumatoid factor.", "contents": "Systemic sclerosis with subcutaneous nodules. A case of systemic sclerosis with subcutaneous nodules is described. The nodules consisted of fibrinoid degeneration with surrounding fibrosis, but lacked the typical histiocytic palisade of the rheumatoid nodule. The patient had neither coexistent rheumatoid arthritis nor circulating rheumatoid factor."}
{"id": "PMID:475995", "title": "Variations in granulocyte colony forming cell numbers in adult blood.", "content": "Blood granulocyte colony forming units (CFU-C) were studied in normal adults to establish: (i) a normal range; (ii) variability due to the culture technique; (iii) variability of blood CFU-C within individuals. Thirty men studied had 98 (range 8--300) CFU-C X 10(3)/1, and 28 women studied had 44 (range 0--260) CFU-C X 10(3)/1. This difference was significant (P less than 0.001). There was also a significant sex difference in the total number of cells forming colonies and clusters per litre; and in the incidence of colony formers and of cluster formers in buffy coat and mononuclear cell blood fractions. CFU-C were assayed in four subjects over a 10 week period. When buffy coat cells were used as a source of colony stimulation the week to week variation in the combined growth of the four subjects was wide (+/- 36%) but with conditioned medium the variation was smaller (+/- 14%). In all subjects colony and cluster growth varied in the same way (r = 0.77, P = .001) but there was no correlation with the total leucocyte count. A 3--4 week cyclical change in CFU-C/1 was found which was independent of the variation inherent in the technique. The physiological significance of the sex difference and the apparent cyclical changes in blood CFU-C are not explained, but the results emphasize the wide fluctuations in CFU-C that may occur in normal individuals.", "contents": "Variations in granulocyte colony forming cell numbers in adult blood. Blood granulocyte colony forming units (CFU-C) were studied in normal adults to establish: (i) a normal range; (ii) variability due to the culture technique; (iii) variability of blood CFU-C within individuals. Thirty men studied had 98 (range 8--300) CFU-C X 10(3)/1, and 28 women studied had 44 (range 0--260) CFU-C X 10(3)/1. This difference was significant (P less than 0.001). There was also a significant sex difference in the total number of cells forming colonies and clusters per litre; and in the incidence of colony formers and of cluster formers in buffy coat and mononuclear cell blood fractions. CFU-C were assayed in four subjects over a 10 week period. When buffy coat cells were used as a source of colony stimulation the week to week variation in the combined growth of the four subjects was wide (+/- 36%) but with conditioned medium the variation was smaller (+/- 14%). In all subjects colony and cluster growth varied in the same way (r = 0.77, P = .001) but there was no correlation with the total leucocyte count. A 3--4 week cyclical change in CFU-C/1 was found which was independent of the variation inherent in the technique. The physiological significance of the sex difference and the apparent cyclical changes in blood CFU-C are not explained, but the results emphasize the wide fluctuations in CFU-C that may occur in normal individuals."}
{"id": "PMID:475996", "title": "Effect of antilymphocyte globulin on granulocyte precursors in aplastic anaemia.", "content": "Granylocyte colony forming units (CFU-C) were studied in 22 patients with severe aplastic anaemia before and after treatment with antilymphocyte globulin (ALG). Nine patients showed a clinical response to ALG characterized by a rise in the granulocyte count to over I X 10(9)/1 within 30 d. These patients were distinguished in vitro from non-responders by an increase in CFU-C numbers after incubation of bone marrow cells with ALG, and by the presence of inhibitors of normal CFU-C in the serum in six out of seven patients tested. In responding patients bone marrow CFU-C rose while most non-responding patients showed no change or a fall in CFU-C after treatment. In addition in three out of four responding patients examined serum inhibitors disappeared after treatment. The horse ALG used in this study also stimulated normal CFU-C in vitro. This evidence is contrary to the hypothesis that ALG stimulates CFU-C in aplasia by inactivating an abnormal suppressor lymphocyte population. The nature of the stimulation by ALG remains unclear. But in practice the effect of ALG on bone marrow cells and study of CFU-C inhibitors in serum could be used to select patients likely to respond to ALG treatment.", "contents": "Effect of antilymphocyte globulin on granulocyte precursors in aplastic anaemia. Granylocyte colony forming units (CFU-C) were studied in 22 patients with severe aplastic anaemia before and after treatment with antilymphocyte globulin (ALG). Nine patients showed a clinical response to ALG characterized by a rise in the granulocyte count to over I X 10(9)/1 within 30 d. These patients were distinguished in vitro from non-responders by an increase in CFU-C numbers after incubation of bone marrow cells with ALG, and by the presence of inhibitors of normal CFU-C in the serum in six out of seven patients tested. In responding patients bone marrow CFU-C rose while most non-responding patients showed no change or a fall in CFU-C after treatment. In addition in three out of four responding patients examined serum inhibitors disappeared after treatment. The horse ALG used in this study also stimulated normal CFU-C in vitro. This evidence is contrary to the hypothesis that ALG stimulates CFU-C in aplasia by inactivating an abnormal suppressor lymphocyte population. The nature of the stimulation by ALG remains unclear. But in practice the effect of ALG on bone marrow cells and study of CFU-C inhibitors in serum could be used to select patients likely to respond to ALG treatment."}
{"id": "PMID:475997", "title": "Red blood cell enzyme abnormalities in patients treated with chemotherapy.", "content": "Sxiteen red blood cell enzyme activities and fetal haemoglobin level have been assayed in 60 patients treated for haematologic or nonhaematologic malignant diseases with various combinations of cytostatic drugs. Acquired enzyme deficiency was found in 20 patients. The most frequently decreased activities were those of G6PD (12 cases), PK (seven cases), PFK (six cases) and AK (three cases). In many patients decreased activity of some enzymes contrasted with increased activity of others such as ALD, TPI, G3PD, PGK, ENOL and 6PGD. The number of abnormalities seems to be related to the duration of the treatment. Incidence of enzyme deficiencies was similar in patients previously treated or not with radiotherapy. Enzyme abnormalities were correlated neither with haemoglobin level nor with reticulocyte count. They were very similar to those observed in primary acquired dyserythropoietic and preleukaemic states. Their prognostic value and mechanism are discussed.", "contents": "Red blood cell enzyme abnormalities in patients treated with chemotherapy. Sxiteen red blood cell enzyme activities and fetal haemoglobin level have been assayed in 60 patients treated for haematologic or nonhaematologic malignant diseases with various combinations of cytostatic drugs. Acquired enzyme deficiency was found in 20 patients. The most frequently decreased activities were those of G6PD (12 cases), PK (seven cases), PFK (six cases) and AK (three cases). In many patients decreased activity of some enzymes contrasted with increased activity of others such as ALD, TPI, G3PD, PGK, ENOL and 6PGD. The number of abnormalities seems to be related to the duration of the treatment. Incidence of enzyme deficiencies was similar in patients previously treated or not with radiotherapy. Enzyme abnormalities were correlated neither with haemoglobin level nor with reticulocyte count. They were very similar to those observed in primary acquired dyserythropoietic and preleukaemic states. Their prognostic value and mechanism are discussed."}
{"id": "PMID:475998", "title": "Fatty acid composition of erythrocytes in hereditary spherocytosis.", "content": "The fatty acid distribution of the four major phospholipids has been determined in the erythrocytes of five patients with hereditary spherocytosis and in five control subjects. In contrast to a report by other workers, we are unable to confirm their findings of a defect in fatty acid chain lengthening activity leading to disappearance of long chain fatty acid conjugates of more than 20 carbon atoms in some of the phospholipid fractions.", "contents": "Fatty acid composition of erythrocytes in hereditary spherocytosis. The fatty acid distribution of the four major phospholipids has been determined in the erythrocytes of five patients with hereditary spherocytosis and in five control subjects. In contrast to a report by other workers, we are unable to confirm their findings of a defect in fatty acid chain lengthening activity leading to disappearance of long chain fatty acid conjugates of more than 20 carbon atoms in some of the phospholipid fractions."}
{"id": "PMID:475999", "title": "Detection of Haemophilia A carriers by replicate factor VIII activity and factor VIII antigenicity determinations.", "content": "Factor VIII (fVIII) activity and antigenicity were determined on replicate fresh blood samples drawn on three separate occasions in 48 normal females and 37 obligatory haemophilia A carriers, in an attempt to improve the detection rate. fVIII activity was assayed by a one-stage method and fVIII antigenicity by the Laurell's technique employing an anti-fVIII antiserum prepared in rabbits. The mean results for the individual subjects were analysed after establishing a discriminant function. The probability of being a carrier was determined on the basis of laboratory data and family history. The probability data were translated into more simple terms applicable for genetic counselling by defining three categories: Definite carriers, doubtful carriers and definite normals. Of the obligatory carriers, 83.8% were classified definite carriers, 13.5% doubtful carriers and 2.7% as definite normals. Of the normal females, 4.2% were classified as definite carriers, 18.7% doubtful carriers and 77.1% as definite normals. Adjustment of the results according to age was found unnecessary. The precision of the determinations of fVIII antigenicity and fVIII activity in individual subjects was calculated for different numbers of replicate tests performed in the same individual. It was found that if only one test is performed, a substantial error might occur. Three replicate tests considerably diminish this error, although it is still in the range of +/- 16--29%. For suspected carriers who fall in the doubtful category it is suggested that six or even more tests are performed.", "contents": "Detection of Haemophilia A carriers by replicate factor VIII activity and factor VIII antigenicity determinations. Factor VIII (fVIII) activity and antigenicity were determined on replicate fresh blood samples drawn on three separate occasions in 48 normal females and 37 obligatory haemophilia A carriers, in an attempt to improve the detection rate. fVIII activity was assayed by a one-stage method and fVIII antigenicity by the Laurell's technique employing an anti-fVIII antiserum prepared in rabbits. The mean results for the individual subjects were analysed after establishing a discriminant function. The probability of being a carrier was determined on the basis of laboratory data and family history. The probability data were translated into more simple terms applicable for genetic counselling by defining three categories: Definite carriers, doubtful carriers and definite normals. Of the obligatory carriers, 83.8% were classified definite carriers, 13.5% doubtful carriers and 2.7% as definite normals. Of the normal females, 4.2% were classified as definite carriers, 18.7% doubtful carriers and 77.1% as definite normals. Adjustment of the results according to age was found unnecessary. The precision of the determinations of fVIII antigenicity and fVIII activity in individual subjects was calculated for different numbers of replicate tests performed in the same individual. It was found that if only one test is performed, a substantial error might occur. Three replicate tests considerably diminish this error, although it is still in the range of +/- 16--29%. For suspected carriers who fall in the doubtful category it is suggested that six or even more tests are performed."}
{"id": "PMID:476000", "title": "Coagulation activation and hyperviscosity in infection.", "content": "A serial study of coagulation activation and whole-blood viscosity was performed on 37 patients with local or systemic bacterial infection, malaria, or a viral infection. Thrombocytopenia, without consumption of coagulation factors, was the main feature of benign tertian malaria and viral infection, whereas in septicaemia and malignant tertian malaria it was associated with activation of coagulation and fibrinolysis. Patients with evidence of intravascular coagulation showed the highest levels of factor VIII related antigen which did not correlate with fibrinogen and probably reflected vascular endothelial cell damage rather than an acute-phase protein reaction. Hyperviscosity, which has been implicated in the pathogenesis of endotoxic shock and cerebral malaria, occurred in parallel with the acute-phase rise in plasma fibrinogen. There was, however, no evidence to implicate hyperviscosity as a major causative factor in the pathogenesis of septic shock or severe infective illness.", "contents": "Coagulation activation and hyperviscosity in infection. A serial study of coagulation activation and whole-blood viscosity was performed on 37 patients with local or systemic bacterial infection, malaria, or a viral infection. Thrombocytopenia, without consumption of coagulation factors, was the main feature of benign tertian malaria and viral infection, whereas in septicaemia and malignant tertian malaria it was associated with activation of coagulation and fibrinolysis. Patients with evidence of intravascular coagulation showed the highest levels of factor VIII related antigen which did not correlate with fibrinogen and probably reflected vascular endothelial cell damage rather than an acute-phase protein reaction. Hyperviscosity, which has been implicated in the pathogenesis of endotoxic shock and cerebral malaria, occurred in parallel with the acute-phase rise in plasma fibrinogen. There was, however, no evidence to implicate hyperviscosity as a major causative factor in the pathogenesis of septic shock or severe infective illness."}
{"id": "PMID:476004", "title": "The effect of nitrous oxide on the cell cycle in human bone marrow.", "content": "The effect of 24 h exposure to nitrous oxide on the cell division cycle in human bone marrow has been studied in vivo using the technique of DNA flow microfluorimetry. All patients who received nitrous oxide showed a significant increase in the proportion of early S-phase cells with a decrease in late S, G2 and mitotic cells. These changes resemble those seen following the use of S-phase-specific cytotoxic drugs. Control patients showed no such effect. Parallel studies have suggested that interference with the function of vitamin B12 underlies this response. Nitrous oxide may provide a convenient method for studying the cell kinetic aspects of acute B12 deficiency and the possibility of using it to increase the therapeutic index of antitumour drugs is discussed.", "contents": "The effect of nitrous oxide on the cell cycle in human bone marrow. The effect of 24 h exposure to nitrous oxide on the cell division cycle in human bone marrow has been studied in vivo using the technique of DNA flow microfluorimetry. All patients who received nitrous oxide showed a significant increase in the proportion of early S-phase cells with a decrease in late S, G2 and mitotic cells. These changes resemble those seen following the use of S-phase-specific cytotoxic drugs. Control patients showed no such effect. Parallel studies have suggested that interference with the function of vitamin B12 underlies this response. Nitrous oxide may provide a convenient method for studying the cell kinetic aspects of acute B12 deficiency and the possibility of using it to increase the therapeutic index of antitumour drugs is discussed."}
{"id": "PMID:476005", "title": "The kinetics of serum and tissue ferritins: relation to carbohydrate content.", "content": "Significant differences were observed in the rate of disappearance from plasma of ferritins purified from rat serum and from different organs. Ferritin from all sources including purified serum ferritin was rapidly removed from plasma by the liver. No difference in biological half-life was observed between apoferritin prepared by ultracentrifugation of liver ferritin and whole liver ferritin and iron-loaded animals cleared injected serum ferritin from plasma at a comparable rate to normal rats. When amounts of 100 microgram of ferritin were injected into rats the half-life was significantly lengthened. The study confirmed the fact that ferritin iron and ferritin protein were removed from plasma at the same rate. No consistent effect of acidic or more basic isoferritin composition on biological half-life was apparent. After chromatography on concanavalin A-Sepharose 6B those ferritins which were predominantly bound to Con A-Sepharose had a half-life which was approximately twice that of ferritins which did not bind. It is concluded that the variation in plasma disappearance of ferritins of different tissue origin was explainable on the basis of carbohydrate content of the molecule.", "contents": "The kinetics of serum and tissue ferritins: relation to carbohydrate content. Significant differences were observed in the rate of disappearance from plasma of ferritins purified from rat serum and from different organs. Ferritin from all sources including purified serum ferritin was rapidly removed from plasma by the liver. No difference in biological half-life was observed between apoferritin prepared by ultracentrifugation of liver ferritin and whole liver ferritin and iron-loaded animals cleared injected serum ferritin from plasma at a comparable rate to normal rats. When amounts of 100 microgram of ferritin were injected into rats the half-life was significantly lengthened. The study confirmed the fact that ferritin iron and ferritin protein were removed from plasma at the same rate. No consistent effect of acidic or more basic isoferritin composition on biological half-life was apparent. After chromatography on concanavalin A-Sepharose 6B those ferritins which were predominantly bound to Con A-Sepharose had a half-life which was approximately twice that of ferritins which did not bind. It is concluded that the variation in plasma disappearance of ferritins of different tissue origin was explainable on the basis of carbohydrate content of the molecule."}
{"id": "PMID:476006", "title": "Studies in desferrioxamine and ferrioxamine metabolism in normal and iron-loaded subjects.", "content": "Plasma concentrations of desferrioxamine and ferrioxamine were measured following bolus injections of desferrioxamine and during 24 h infusions of the drug. [59Fe]ferrioxamine clearance and urinary iron excretion were also measured. Higher plasma ferrioxamine concentrations were found in iron loaded subjects and higher desferrioxamine concentrations in subjects with normal ironloads. There is a correlation between the circulating concentration of ferrioxamine during an infusion and the 48 h urinary iron excretion. The data suggests that the amount of iron chelated in vivo is related to an increase in the size of an intermediate chelatable pool rather than the total amount of the iron load. The well-recognized delay in urinary iron excretion appears to be related to active tubular reabsorption of ferrioxamine.", "contents": "Studies in desferrioxamine and ferrioxamine metabolism in normal and iron-loaded subjects. Plasma concentrations of desferrioxamine and ferrioxamine were measured following bolus injections of desferrioxamine and during 24 h infusions of the drug. [59Fe]ferrioxamine clearance and urinary iron excretion were also measured. Higher plasma ferrioxamine concentrations were found in iron loaded subjects and higher desferrioxamine concentrations in subjects with normal ironloads. There is a correlation between the circulating concentration of ferrioxamine during an infusion and the 48 h urinary iron excretion. The data suggests that the amount of iron chelated in vivo is related to an increase in the size of an intermediate chelatable pool rather than the total amount of the iron load. The well-recognized delay in urinary iron excretion appears to be related to active tubular reabsorption of ferrioxamine."}
{"id": "PMID:476007", "title": "Haemoglobin Lepore trait: haematological and structural studies on the Italian population.", "content": "Haematological data on 59 heterozygotes for haemoglobin (Hb) Lepore and 10 double heterozygotes for Hb Lepore and beta thalassaemia from 36 Italian families are reported. The red cell indices are defined and compared with those of groups of non-thalassaemic and beta thalassaemic subjects of comparable number, age and sex distribution. The relative level of each haemoglobin fraction and the absolute production of single polypeptide chains are calculated in order to compare the expression of the non-alpha chain genes in Hb Lepore trait and beta thalassaemia. Structural studies demonstrate that the haemoglobin Lepore is of the Boston type (delta 87 beta 116) in all subjects, confirming that this type of fusion variant is probably the only one which occurs in Mediterranean populations. The distribution and incidence of the Lepore haemoglobinopathy are discussed.", "contents": "Haemoglobin Lepore trait: haematological and structural studies on the Italian population. Haematological data on 59 heterozygotes for haemoglobin (Hb) Lepore and 10 double heterozygotes for Hb Lepore and beta thalassaemia from 36 Italian families are reported. The red cell indices are defined and compared with those of groups of non-thalassaemic and beta thalassaemic subjects of comparable number, age and sex distribution. The relative level of each haemoglobin fraction and the absolute production of single polypeptide chains are calculated in order to compare the expression of the non-alpha chain genes in Hb Lepore trait and beta thalassaemia. Structural studies demonstrate that the haemoglobin Lepore is of the Boston type (delta 87 beta 116) in all subjects, confirming that this type of fusion variant is probably the only one which occurs in Mediterranean populations. The distribution and incidence of the Lepore haemoglobinopathy are discussed."}
{"id": "PMID:476008", "title": "Neonatal erythrocyte glutathione peroxidase deficiency as a consequence of selenium imbalance during pregnancy.", "content": "The red blood cell (RBC) glutathione peroxidase (GSH-Px) activity and routine haematological parameters were measured in 38 healthy north Italian full-term pregnant women and in their newborn infants. In 31 pairs the serum selenium concentration was also measured. Data were compared with those of 20 normal adult controls (10 males and 10 females). Newborn infants exhibited significantly lower RBC GSH-Px activity and serum selenium concentrations than adult controls. Pregnant women had serum selenium values intermediate between those of adult female controls and their newborn infants. In both the pregnant women and newborns the RBC GSH-Px activity correlated with the level of selenium in serum which suggests that the neonatal RBC GSH-Px deficiency may be partially due to insufficient availability of selenium during pregancy. Factors other than selenium concentration, e.g. hormonal and genetic, might also affect the RBC GSH-Px activity as suggested by sex differences and mother/child concordances in enzyme activity found in our cases.", "contents": "Neonatal erythrocyte glutathione peroxidase deficiency as a consequence of selenium imbalance during pregnancy. The red blood cell (RBC) glutathione peroxidase (GSH-Px) activity and routine haematological parameters were measured in 38 healthy north Italian full-term pregnant women and in their newborn infants. In 31 pairs the serum selenium concentration was also measured. Data were compared with those of 20 normal adult controls (10 males and 10 females). Newborn infants exhibited significantly lower RBC GSH-Px activity and serum selenium concentrations than adult controls. Pregnant women had serum selenium values intermediate between those of adult female controls and their newborn infants. In both the pregnant women and newborns the RBC GSH-Px activity correlated with the level of selenium in serum which suggests that the neonatal RBC GSH-Px deficiency may be partially due to insufficient availability of selenium during pregancy. Factors other than selenium concentration, e.g. hormonal and genetic, might also affect the RBC GSH-Px activity as suggested by sex differences and mother/child concordances in enzyme activity found in our cases."}
{"id": "PMID:476009", "title": "Hereditary acanthocytosis associated with the McLeod phenotype of the Kell blood group system.", "content": "Some boys with X-linked chronic granulomatous disease (CGD) have red cells of the rare McLeod phenotype in the Kell blood group system. Only one example of this phenotype has previously been described in a non-CGD subject. We have studied a 10-year-old boy and a maternal brother who do not have CGD and whose red cells are of the McLeod type . The boy presented as a haematological problem with red-cell abnormalities. These were acanthocytosis, anisocytosis and 'tailing' in the osmotic fragility curve, changes now known to occur with the McLeod phenotype. Subsequent studies revealed his rare blood group. A family study has established that an uncle also has acanthocytic red cells and the McLeod phenotype. In addition the boy's sister, mother and maternal grandmother all show red-cell mosaicism with double populations of McLeod acanthocytes and normal red cells of common Kell type. The gene that determines inheritance of the McLeod phenotype is X-linked and the mosaicism present in female carriers is believed to result from X chromosome inactivation by the Lyon effect. The study provides further evidence that the McLeod phenotype arises by inheritance of a variant X-linked modifying gene and not through inheritance of a variant gene at the Kell autosomal locus. It also represents the first occasion that a person of rare blood group has been recognized because of an associated anomaly in red cell morphology.", "contents": "Hereditary acanthocytosis associated with the McLeod phenotype of the Kell blood group system. Some boys with X-linked chronic granulomatous disease (CGD) have red cells of the rare McLeod phenotype in the Kell blood group system. Only one example of this phenotype has previously been described in a non-CGD subject. We have studied a 10-year-old boy and a maternal brother who do not have CGD and whose red cells are of the McLeod type . The boy presented as a haematological problem with red-cell abnormalities. These were acanthocytosis, anisocytosis and 'tailing' in the osmotic fragility curve, changes now known to occur with the McLeod phenotype. Subsequent studies revealed his rare blood group. A family study has established that an uncle also has acanthocytic red cells and the McLeod phenotype. In addition the boy's sister, mother and maternal grandmother all show red-cell mosaicism with double populations of McLeod acanthocytes and normal red cells of common Kell type. The gene that determines inheritance of the McLeod phenotype is X-linked and the mosaicism present in female carriers is believed to result from X chromosome inactivation by the Lyon effect. The study provides further evidence that the McLeod phenotype arises by inheritance of a variant X-linked modifying gene and not through inheritance of a variant gene at the Kell autosomal locus. It also represents the first occasion that a person of rare blood group has been recognized because of an associated anomaly in red cell morphology."}
{"id": "PMID:476011", "title": "Coagulation abnormalities produced by plasma exchange on the cell separator with special reference to fibrinogen and platelet levels.", "content": "Five patients with immunopathologic renal disease, 12 with malignant paraproteinaemia and one with myasthenia gravis underwent a total of 179 plasma exchanges on a continuous flow cell separator. Replacement fluids devoid of coagulation factors were used in 160 exchanges while 19 exchanges were replaced with Fresh Frozen Plasma. Coagulation screening was done immediately before and 30 min after each plasma exchange. Plasma fibrinogen concentrations fell to a mean of 25% of initial levels during individual exchanges. Sequential reduction to 10.7% was observed after five consecutive daily exchanges and in one patient fell to 1.2% after 10 daily exchanges. Low levels of fibrinogen could be maintained with daily or alternate daily exchanges. Platelet counts fell to a mean of 50% of pre-exchange levels during individual exchanges. Consecutive daily exchanges resulted in mean reductions to 20.7% after 5 d, but further reductions were not observed with longer periods of exchange. Platelet counts recovered to pre-exchange values during exchange intervals of 2 or more days. Mild clinical bleeding episodes, probably related to low platelet counts, occurred in one exchange in each of three patients. Haemostasis was rapidly achieved in these patients by infusions of platelet concentrates. Coagulation screening, including prothrombin ratio, thrombin time, reptilase time and partial thromboplastin time with kaolin showed progressively greater abnormalities as the intervals between exchanges shortened. The low incidence of clinical haemorrhagic episodes, three of 179 exchanges (2.2%), despite markedly abnormal coagulation parameters, emphasize the safety of the procedure even in moribund patients. We conclude that the use of FFP in intensive exchange programmes solely for the prevention of spontaneous haemorrhagic phenoma is unjustified.", "contents": "Coagulation abnormalities produced by plasma exchange on the cell separator with special reference to fibrinogen and platelet levels. Five patients with immunopathologic renal disease, 12 with malignant paraproteinaemia and one with myasthenia gravis underwent a total of 179 plasma exchanges on a continuous flow cell separator. Replacement fluids devoid of coagulation factors were used in 160 exchanges while 19 exchanges were replaced with Fresh Frozen Plasma. Coagulation screening was done immediately before and 30 min after each plasma exchange. Plasma fibrinogen concentrations fell to a mean of 25% of initial levels during individual exchanges. Sequential reduction to 10.7% was observed after five consecutive daily exchanges and in one patient fell to 1.2% after 10 daily exchanges. Low levels of fibrinogen could be maintained with daily or alternate daily exchanges. Platelet counts fell to a mean of 50% of pre-exchange levels during individual exchanges. Consecutive daily exchanges resulted in mean reductions to 20.7% after 5 d, but further reductions were not observed with longer periods of exchange. Platelet counts recovered to pre-exchange values during exchange intervals of 2 or more days. Mild clinical bleeding episodes, probably related to low platelet counts, occurred in one exchange in each of three patients. Haemostasis was rapidly achieved in these patients by infusions of platelet concentrates. Coagulation screening, including prothrombin ratio, thrombin time, reptilase time and partial thromboplastin time with kaolin showed progressively greater abnormalities as the intervals between exchanges shortened. The low incidence of clinical haemorrhagic episodes, three of 179 exchanges (2.2%), despite markedly abnormal coagulation parameters, emphasize the safety of the procedure even in moribund patients. We conclude that the use of FFP in intensive exchange programmes solely for the prevention of spontaneous haemorrhagic phenoma is unjustified."}
{"id": "PMID:476013", "title": "A genetic variant of factor IX with decreased capacity for Ca2+ binding.", "content": "A genetic variant of factor IX is described that behaves identically to PIVKA/IX (the precursor factor IX molecule induced by the absence of vitamin K or presence of vitamin K antagonists, acarboxy factor IX). It shows an increased electrophoretic mobility in the presence of Ca2+, a low affinity for adsorption to A1(OH)3 and a very low specific coagulant activity. This variant of factor IX has been demonstrated in the plasma of a patient with severe haemophilia B and in the plasmas of a number of possible carriers from the probands' pedigree.", "contents": "A genetic variant of factor IX with decreased capacity for Ca2+ binding. A genetic variant of factor IX is described that behaves identically to PIVKA/IX (the precursor factor IX molecule induced by the absence of vitamin K or presence of vitamin K antagonists, acarboxy factor IX). It shows an increased electrophoretic mobility in the presence of Ca2+, a low affinity for adsorption to A1(OH)3 and a very low specific coagulant activity. This variant of factor IX has been demonstrated in the plasma of a patient with severe haemophilia B and in the plasmas of a number of possible carriers from the probands' pedigree."}
{"id": "PMID:476012", "title": "Indium-III: a new radionuclide label for studying human platelet kinetics.", "content": "Indium-III. when complexed with 8-hydroxyquinoline (oxine), has been employed as a radioactive platelet label for thrombus imaging in animals and man. The short half-life (2.8 d) and high yield of gamma photons of 111In make it ideal for in vitro counting and external imaging. To evaluate its suitability for studies of platelet turnover in man, platelet kinetic studies were carried out on 10 healthy volunteers using 111In-and 51Cr-platelets concurrently. For 111In labelling, platelets were harvested by differential centrifugation from 43 ml of whole blood drawn into acid-citrate dextrose (ACD) solution. The platelets were washed and suspended in a mixture of ACD and isotonic saline and then incubated with 111In-oxine, rewashed, and suspended in plasma for reinfusion. 51Cr labelling was performed using standard methods. Mean labelling efficiency was 73% with 111In and 6.5% with 51Cr. In vitro studies demonstrated minimal release, elution, and reutilization of the 111In label. There was no significant difference in the aggregation response of 111In- and 51Cr-platelets to ADP and collagen. The in vivo recovery of 111In-platelets was approximately 50% greater than that of 51Cr-platelets whereas the platelet life spans were similar. These results indicate that 111In labelled platelets may be useful for thromobokinetic studies in man. The new method offers the advantages of reduced blood requirements, higher labelling efficiency, and the ability to perform external imaging of platelet distribution in vivo.", "contents": "Indium-III: a new radionuclide label for studying human platelet kinetics. Indium-III. when complexed with 8-hydroxyquinoline (oxine), has been employed as a radioactive platelet label for thrombus imaging in animals and man. The short half-life (2.8 d) and high yield of gamma photons of 111In make it ideal for in vitro counting and external imaging. To evaluate its suitability for studies of platelet turnover in man, platelet kinetic studies were carried out on 10 healthy volunteers using 111In-and 51Cr-platelets concurrently. For 111In labelling, platelets were harvested by differential centrifugation from 43 ml of whole blood drawn into acid-citrate dextrose (ACD) solution. The platelets were washed and suspended in a mixture of ACD and isotonic saline and then incubated with 111In-oxine, rewashed, and suspended in plasma for reinfusion. 51Cr labelling was performed using standard methods. Mean labelling efficiency was 73% with 111In and 6.5% with 51Cr. In vitro studies demonstrated minimal release, elution, and reutilization of the 111In label. There was no significant difference in the aggregation response of 111In- and 51Cr-platelets to ADP and collagen. The in vivo recovery of 111In-platelets was approximately 50% greater than that of 51Cr-platelets whereas the platelet life spans were similar. These results indicate that 111In labelled platelets may be useful for thromobokinetic studies in man. The new method offers the advantages of reduced blood requirements, higher labelling efficiency, and the ability to perform external imaging of platelet distribution in vivo."}
{"id": "PMID:476014", "title": "The treatment of advanced cystadenocarcinoma of the ovary with gestronol and continuous oral cyclophosphamide.", "content": "Fifty-four patients with advanced cystadenocarcinoma of the ovary were treated with gestronol (Depostat), 200 mg/week, by intramuscular injection and continuous oral cyclophosphamide, the dose of the latter being varied to maintain the patient's white cell count at about 1.5 x 10(9)/l. A combined partial and complete remission rate of 76 per cent was obtained. The length of survival depended upon the type of remission and the type of remission was markedly affected by the amount of tumour removed at initial laparotomy.", "contents": "The treatment of advanced cystadenocarcinoma of the ovary with gestronol and continuous oral cyclophosphamide. Fifty-four patients with advanced cystadenocarcinoma of the ovary were treated with gestronol (Depostat), 200 mg/week, by intramuscular injection and continuous oral cyclophosphamide, the dose of the latter being varied to maintain the patient's white cell count at about 1.5 x 10(9)/l. A combined partial and complete remission rate of 76 per cent was obtained. The length of survival depended upon the type of remission and the type of remission was markedly affected by the amount of tumour removed at initial laparotomy."}
{"id": "PMID:476015", "title": "Hormonal profiles in lactating and non-lactating women immediately after delivery and their relationship to breast engorgement.", "content": "Prolactin, human placental lactogen (HPL), oestrone, oestradiol and progesterone levels in plasma were measured before and during the first seven days after delivery in women who did not breast feed. The results confirmed the rapid clearance of placental steroids from the circulation after delivery. Plasma prolactin levels remained elevated during the early puerperium and the range of values were the same in non breast-feeding women and a group of breast feeding women. Of the 25 women studied, six developed breast engorgement. No difference in hormonal profiles were found leading to the conclusion that there is no endocrine basis for breast engorgement in non-breast feeding women.", "contents": "Hormonal profiles in lactating and non-lactating women immediately after delivery and their relationship to breast engorgement. Prolactin, human placental lactogen (HPL), oestrone, oestradiol and progesterone levels in plasma were measured before and during the first seven days after delivery in women who did not breast feed. The results confirmed the rapid clearance of placental steroids from the circulation after delivery. Plasma prolactin levels remained elevated during the early puerperium and the range of values were the same in non breast-feeding women and a group of breast feeding women. Of the 25 women studied, six developed breast engorgement. No difference in hormonal profiles were found leading to the conclusion that there is no endocrine basis for breast engorgement in non-breast feeding women."}
{"id": "PMID:476016", "title": "A comparison of crown-rump length measurements using a real-time scanner in an antenatal clinic and a conventional B-scanner.", "content": "In a series of 168 patients in the first trimester of pregnancy, fetal crown-rump length (CRL) measurements were attempted in the Antenatal Clinic using a linear-array real-time ultrasound scanner. The measurements could not be made in 23 of the patients; most of them had a gestational age of less than 10 weeks and the difficulty in obtaining measurements was usually due to an underfilled maternal bladder. When compared with CRL measurements obtained with a conventional B-scanner, a good correlation was obtained (r = 0.92); 80 per cent of the 'real-time' values fell within a range of +/- 5 mm of the conventional values. In all patients the estimates of gestational age using the two methods were within one week of each other.", "contents": "A comparison of crown-rump length measurements using a real-time scanner in an antenatal clinic and a conventional B-scanner. In a series of 168 patients in the first trimester of pregnancy, fetal crown-rump length (CRL) measurements were attempted in the Antenatal Clinic using a linear-array real-time ultrasound scanner. The measurements could not be made in 23 of the patients; most of them had a gestational age of less than 10 weeks and the difficulty in obtaining measurements was usually due to an underfilled maternal bladder. When compared with CRL measurements obtained with a conventional B-scanner, a good correlation was obtained (r = 0.92); 80 per cent of the 'real-time' values fell within a range of +/- 5 mm of the conventional values. In all patients the estimates of gestational age using the two methods were within one week of each other."}
{"id": "PMID:476017", "title": "The accuracy of radiological estimates of gestational age using early fetal crown-rump length measurements by ultrasound as a basis for comparison.", "content": "Radiological estimates of gestational age in late pregnancy were compared with the gestational ages based on first trimester ultrasound crown-rump length measurements in a series of 125 patients. It was found that the 95 per cent confidence limits of a radiological estimate were +/- 3 1/2 weeks, that both distal femoral and upper tibial epiphyses were frequently present much earlier than 36 and 38 weeks respectively, and that in almost half of the growth-retarded babies in the series there was a bone-age discrepancy of three or more weeks. It is recommended that more liberal use is made of early ultrasound measurements for the assessment of gestational age.", "contents": "The accuracy of radiological estimates of gestational age using early fetal crown-rump length measurements by ultrasound as a basis for comparison. Radiological estimates of gestational age in late pregnancy were compared with the gestational ages based on first trimester ultrasound crown-rump length measurements in a series of 125 patients. It was found that the 95 per cent confidence limits of a radiological estimate were +/- 3 1/2 weeks, that both distal femoral and upper tibial epiphyses were frequently present much earlier than 36 and 38 weeks respectively, and that in almost half of the growth-retarded babies in the series there was a bone-age discrepancy of three or more weeks. It is recommended that more liberal use is made of early ultrasound measurements for the assessment of gestational age."}
{"id": "PMID:476018", "title": "Routine induction of labour with extra-amniotic prostaglandin E2 in a viscous gel.", "content": "Prostaglandin E2, 350 microgram dispersed in a viscous gel, tylose, was introduced into the extra-amniotic space as a single dose in 285 patients to induce labour. With a favourable cervix, 82 per cent of multiparae and 50 per cent of primiparae were successfully induced. With unfavourable induction features, the success rates were 48 per cent and 24 per cent respectively. In the remaining patients, all but four were successfully delivered when intravenous oxytocin was also used. The method was safe, simple and inexpensive and had many advantages for patients and nursing staff.", "contents": "Routine induction of labour with extra-amniotic prostaglandin E2 in a viscous gel. Prostaglandin E2, 350 microgram dispersed in a viscous gel, tylose, was introduced into the extra-amniotic space as a single dose in 285 patients to induce labour. With a favourable cervix, 82 per cent of multiparae and 50 per cent of primiparae were successfully induced. With unfavourable induction features, the success rates were 48 per cent and 24 per cent respectively. In the remaining patients, all but four were successfully delivered when intravenous oxytocin was also used. The method was safe, simple and inexpensive and had many advantages for patients and nursing staff."}
{"id": "PMID:476019", "title": "Normal and abnormal antenatal ultrasonic cardiographic patterns.", "content": "Data is presented from 1019 antenatal ultrasonic cardiographs performed on 391 patients; uterine contractions were not monitored concurrently with the fetal heart rate (FHR). No fetal deaths in utero or preterminal FHR patterns were seen within 24 hours of a normal trace. A comprehensive classification of FHR patterns is presented.", "contents": "Normal and abnormal antenatal ultrasonic cardiographic patterns. Data is presented from 1019 antenatal ultrasonic cardiographs performed on 391 patients; uterine contractions were not monitored concurrently with the fetal heart rate (FHR). No fetal deaths in utero or preterminal FHR patterns were seen within 24 hours of a normal trace. A comprehensive classification of FHR patterns is presented."}
{"id": "PMID:476021", "title": "Outcome of pregnancy in women using different methods of contraception.", "content": "During the years 1968-74, 17,032 women were recruited to the Oxford Family Planning Association prospective study of the effects of different methods of contraception. The present analysis, which concerns 5700 pregnancies experienced by the participants in the investigation, adds to the evidence that ex-users of oral contraceptives and intrauterine devices suffer no delecterious effects on the outcome of pregnancy in terms of miscarriage, ectopic gestation, stillbirth, congenital malformation, alteration in the sex ratio or reduction in birth weight. Accidental pregnancies in women taking oral contraceptives were few in the present study (66 in all), but it seems likely that harmful effects of exposure of the fetus to the pill in utero, if any, occur infrequently. Women experiencing an accidental pregnancy with an intrauterine device in situ are at a markedly increased risk of miscarriage and ectopic gestation and are more likely to give birth to an underweight infant than other women.", "contents": "Outcome of pregnancy in women using different methods of contraception. During the years 1968-74, 17,032 women were recruited to the Oxford Family Planning Association prospective study of the effects of different methods of contraception. The present analysis, which concerns 5700 pregnancies experienced by the participants in the investigation, adds to the evidence that ex-users of oral contraceptives and intrauterine devices suffer no delecterious effects on the outcome of pregnancy in terms of miscarriage, ectopic gestation, stillbirth, congenital malformation, alteration in the sex ratio or reduction in birth weight. Accidental pregnancies in women taking oral contraceptives were few in the present study (66 in all), but it seems likely that harmful effects of exposure of the fetus to the pill in utero, if any, occur infrequently. Women experiencing an accidental pregnancy with an intrauterine device in situ are at a markedly increased risk of miscarriage and ectopic gestation and are more likely to give birth to an underweight infant than other women."}
{"id": "PMID:476022", "title": "Multiple births in former oral contraceptive users.", "content": "Multiple births were studied in a cohort of 2953 former oral contraceptive users and 13,630 controls, all the women were interviewed post partum. There were 238 multiple births, a rate of 14.4/1000 deliveries. Former oral contraceptive users had 13.5/1000 sets of multiple births compared to 14.5/1000 in the controls. When women who had received treatment for anovulation were excluded, these rates were 11.1 and 12.7/1000 respectively, a difference which is not statistically significant. There was, however, a significant deficit of multiple births among the small group of former pill users who were underweight in relation to their height at the time of conception. Multiple births were also increased after stopping sequential pills and preparations containing high doses of oestrogen, and there was an excess of multiple births following breakthrough pregnancies. These findings, although statistically significant, are based on small numbers, and require confirmation from other studies.", "contents": "Multiple births in former oral contraceptive users. Multiple births were studied in a cohort of 2953 former oral contraceptive users and 13,630 controls, all the women were interviewed post partum. There were 238 multiple births, a rate of 14.4/1000 deliveries. Former oral contraceptive users had 13.5/1000 sets of multiple births compared to 14.5/1000 in the controls. When women who had received treatment for anovulation were excluded, these rates were 11.1 and 12.7/1000 respectively, a difference which is not statistically significant. There was, however, a significant deficit of multiple births among the small group of former pill users who were underweight in relation to their height at the time of conception. Multiple births were also increased after stopping sequential pills and preparations containing high doses of oestrogen, and there was an excess of multiple births following breakthrough pregnancies. These findings, although statistically significant, are based on small numbers, and require confirmation from other studies."}
{"id": "PMID:476023", "title": "A comparison between the effects of nystatin, clotrimazole and miconazole on vaginal candidiasis.", "content": "Nystatin (100 000 units/day for 14 days) and clotrimazole (100 mg/day for 6 days) were equivalent to miconazole (100 mg/day for 14 days or 100 mg twice daily for 7 days) in curing vaginal candidiasis. However significantly more patients relapsed in the nystatin and clotrimazole groups than in either of the miconazole groups.", "contents": "A comparison between the effects of nystatin, clotrimazole and miconazole on vaginal candidiasis. Nystatin (100 000 units/day for 14 days) and clotrimazole (100 mg/day for 6 days) were equivalent to miconazole (100 mg/day for 14 days or 100 mg twice daily for 7 days) in curing vaginal candidiasis. However significantly more patients relapsed in the nystatin and clotrimazole groups than in either of the miconazole groups."}
{"id": "PMID:476024", "title": "Prenatal ultrasound in the diagnosis and management of fetal exomphalos. Case reports.", "content": "A patient is presented in whom ultrasonic examination at 39 weeks detected fetal exomphalos with an intact sac and no associated gross abnormalities. These findings served as major indications for the safe delivery by Caesarean section of a baby that survived and had successful corrective surgery when 6 months old. The infant might well not have survived vaginal delivery.", "contents": "Prenatal ultrasound in the diagnosis and management of fetal exomphalos. Case reports. A patient is presented in whom ultrasonic examination at 39 weeks detected fetal exomphalos with an intact sac and no associated gross abnormalities. These findings served as major indications for the safe delivery by Caesarean section of a baby that survived and had successful corrective surgery when 6 months old. The infant might well not have survived vaginal delivery."}
{"id": "PMID:476027", "title": "Ultrasonic examination in severe diabetic eye disease.", "content": "B-scan ultrasound of the posterior segment is reported in 154 patients with severe diabetic eye disease. Epiretinal fibrosis, vitreous haemorrhage, vitreous detachment, and retinal detachment were frequently diagnosed. The ultrasonic findings are discussed in relation to the pathological changes in the vitreous in proliferative diabetic retinopathy.", "contents": "Ultrasonic examination in severe diabetic eye disease. B-scan ultrasound of the posterior segment is reported in 154 patients with severe diabetic eye disease. Epiretinal fibrosis, vitreous haemorrhage, vitreous detachment, and retinal detachment were frequently diagnosed. The ultrasonic findings are discussed in relation to the pathological changes in the vitreous in proliferative diabetic retinopathy."}
{"id": "PMID:476028", "title": "Dark adaptation in diabetes mellitus.", "content": "The effect of diabetes mellitus on the normal dark adaptation curve is investigated. It has been found that diabetic patients take longer to adapt and that their absolute thresholds are raised. The degree of elevation in the final threshold correlates with the duration of diabetes.", "contents": "Dark adaptation in diabetes mellitus. The effect of diabetes mellitus on the normal dark adaptation curve is investigated. It has been found that diabetic patients take longer to adapt and that their absolute thresholds are raised. The degree of elevation in the final threshold correlates with the duration of diabetes."}
{"id": "PMID:476029", "title": "Blepharochalasis.", "content": "The condition of blepharochalasis and its treatment are discussed. Four cases are presented which show a physical sign that may help in its diagnosis.", "contents": "Blepharochalasis. The condition of blepharochalasis and its treatment are discussed. Four cases are presented which show a physical sign that may help in its diagnosis."}
{"id": "PMID:476030", "title": "Dark adaptation testing in heterozygotes of Usher's syndrome.", "content": "Fourteen heterozygous carriers of Usher's syndrome were evaluated by ophthalmoscopy and dark adaptation testing. The normal findings in our study are discussed and compared with those of previous reports, which suggested that abnormal dark adaptation thresholds and fundus abnormalities may be seen in heterozygotes.", "contents": "Dark adaptation testing in heterozygotes of Usher's syndrome. Fourteen heterozygous carriers of Usher's syndrome were evaluated by ophthalmoscopy and dark adaptation testing. The normal findings in our study are discussed and compared with those of previous reports, which suggested that abnormal dark adaptation thresholds and fundus abnormalities may be seen in heterozygotes."}
{"id": "PMID:476031", "title": "A scanning electron microscopic examination of retinoblastoma in tissue culture.", "content": "Tissue cultures were established from 6 retinoblastomas and the cultured cells examined by scanning electron microscopy. Cells from cultures of normal fetal retinal and glial tissue were also examined by SEM. Though a number of different cell types were found to coexist in the retinoblastoma cultures, most had the features of cells of either glial or neuronal origin. Evidence of further differentiation of cells in each series was seen. These findings indicate that the cell of origin in retinoblastoma is a multipotential stem cell which, though neoplastic, is still capable of differentiation into both glial and neuronal series.", "contents": "A scanning electron microscopic examination of retinoblastoma in tissue culture. Tissue cultures were established from 6 retinoblastomas and the cultured cells examined by scanning electron microscopy. Cells from cultures of normal fetal retinal and glial tissue were also examined by SEM. Though a number of different cell types were found to coexist in the retinoblastoma cultures, most had the features of cells of either glial or neuronal origin. Evidence of further differentiation of cells in each series was seen. These findings indicate that the cell of origin in retinoblastoma is a multipotential stem cell which, though neoplastic, is still capable of differentiation into both glial and neuronal series."}
{"id": "PMID:476032", "title": "Retinoblastoma: correlation of invasion of the optic nerve and choroid with prognosis and metastases.", "content": "The histological sections of 74 retinoblastoma patients were reviewed. The extent of optic nerve invasion was correlated with choroidal/scleral extension. Choroidal invasion carries 100% survival provided that the sclera, iris, and whole optic nerve are not also involved. Invasion of the optic nerve beyond the lamina cribrosa also carries 100% survival provided that the resection line is free and that invasion does not involve the sclera or iris. Plentiful rosettes were usually found in those tumours that had not extended beyond the choroid or as far as the resection line of the optic nerve and were therefore associated with a good prognosis. The absence of rosettes did not necessarily indicate a poor prognosis. The site of metastases was related to optic nerve and scleral/extrascleral extension. The various systems of staging retinoblastoma are compared, and a new system is proposed to cover the whole spectrum of the disease and to offer a reliable guide to prognosis and treatment.", "contents": "Retinoblastoma: correlation of invasion of the optic nerve and choroid with prognosis and metastases. The histological sections of 74 retinoblastoma patients were reviewed. The extent of optic nerve invasion was correlated with choroidal/scleral extension. Choroidal invasion carries 100% survival provided that the sclera, iris, and whole optic nerve are not also involved. Invasion of the optic nerve beyond the lamina cribrosa also carries 100% survival provided that the resection line is free and that invasion does not involve the sclera or iris. Plentiful rosettes were usually found in those tumours that had not extended beyond the choroid or as far as the resection line of the optic nerve and were therefore associated with a good prognosis. The absence of rosettes did not necessarily indicate a poor prognosis. The site of metastases was related to optic nerve and scleral/extrascleral extension. The various systems of staging retinoblastoma are compared, and a new system is proposed to cover the whole spectrum of the disease and to offer a reliable guide to prognosis and treatment."}
{"id": "PMID:476033", "title": "Prognostic significance of aqueous humour lactic dehydrogenase activity.", "content": "The lactic dehydrogenase (LDH) activity of the aqueous humour has been estimated in both eyes of 7 patients having uniocular retinoblastoma. In 1 patient the aqueous humour LDH activity in the healthy eye was above normal, but there was no clinical evidence of malignancy. Tumour tissue was detected in this eye 9 months later, and the aqueous humour showed a rise in LDH activity. A high LDH activity persisted even after irradiation, though no tumour tissue was visible ophthalmoscopically. It is suggested that the estimation of the LDH activity in the aqueous humour of the healthy eye in cases of uniocular retinoblastoma might be of value in the early detection of a bilateral retinoblastoma.", "contents": "Prognostic significance of aqueous humour lactic dehydrogenase activity. The lactic dehydrogenase (LDH) activity of the aqueous humour has been estimated in both eyes of 7 patients having uniocular retinoblastoma. In 1 patient the aqueous humour LDH activity in the healthy eye was above normal, but there was no clinical evidence of malignancy. Tumour tissue was detected in this eye 9 months later, and the aqueous humour showed a rise in LDH activity. A high LDH activity persisted even after irradiation, though no tumour tissue was visible ophthalmoscopically. It is suggested that the estimation of the LDH activity in the aqueous humour of the healthy eye in cases of uniocular retinoblastoma might be of value in the early detection of a bilateral retinoblastoma."}
{"id": "PMID:476034", "title": "Correlation of aqueous humour lactic acid dehydrogenase activity with intraocular pathology.", "content": "An analysis of the lactic acid dehydrogenase (LDH) activity in the aqueous humour of 24 enucleated eyes with retinoblastoma showed that though there was no relationship between the LDH levels and the age of the patient, there appeared to be an increase in the LDH activity with increase in the duration of the tumour. Undifferentiated tumour cells and tumour cell necrosis seemed to contribute to an increase of LDH levels in the aqueous humour, but there was no relationship between the occurrence of calcification and the LDH secretion into the aqueous humour. Massive cell necrosis caused by radiotherapy and central retinal artery occlusion significantly increased the LDH levels in the aqueous. It also appeared that recurrence was common after external cobalt therapy and that secondary extension of the tumour into the optic nerve and choroid was favoured by this procedure.", "contents": "Correlation of aqueous humour lactic acid dehydrogenase activity with intraocular pathology. An analysis of the lactic acid dehydrogenase (LDH) activity in the aqueous humour of 24 enucleated eyes with retinoblastoma showed that though there was no relationship between the LDH levels and the age of the patient, there appeared to be an increase in the LDH activity with increase in the duration of the tumour. Undifferentiated tumour cells and tumour cell necrosis seemed to contribute to an increase of LDH levels in the aqueous humour, but there was no relationship between the occurrence of calcification and the LDH secretion into the aqueous humour. Massive cell necrosis caused by radiotherapy and central retinal artery occlusion significantly increased the LDH levels in the aqueous. It also appeared that recurrence was common after external cobalt therapy and that secondary extension of the tumour into the optic nerve and choroid was favoured by this procedure."}
{"id": "PMID:476035", "title": "A new method for measuring the body density of obese adults.", "content": "1. A new apparatus is described with which it is possible to measure the volume (and hence density) of obese patients without requiring them to immerse totally in water. Replicate measurements of subjects with 6, 23 and 38 kg body fat had a standard deviation not greater than 0.3 kg fat. 2. In nineteen obese women body fat was measured by density, total body water, and total body potassium at the beginning, and again at the end, of a period of 3--4 weeks on a reducing diet, during which they lost 5.43 (SD 1.83) kg in weight. The composition of weight loss was also estimated both by energy balance and nitrogen balance during the interval between the two measurements of body composition. 3. The estimates of fat content of the ninetten women at the start of the balance period were 45.63 (SD 14.50) kg by density, 48.07 (SD 13.88) kg by K and 47.09 (SD 13.85) kg by water. The correlation coefficient between the density and K estimate was 0.949, and for the density and water estimate it was 0.971. 4. It is concluded that measurement of density by the new method provides a convenient method for estimating body fatness, and change in fat content, which compares favourably with estimates based on total body water or total body K. However, these methods cannot be used to provide an accurate estimate of the composition of a small weight loss in an individual since deviations up to 4 kg fat occur between fat loss based on change in density and those based on the more reliable (but more tedious) energy balance method.", "contents": "A new method for measuring the body density of obese adults. 1. A new apparatus is described with which it is possible to measure the volume (and hence density) of obese patients without requiring them to immerse totally in water. Replicate measurements of subjects with 6, 23 and 38 kg body fat had a standard deviation not greater than 0.3 kg fat. 2. In nineteen obese women body fat was measured by density, total body water, and total body potassium at the beginning, and again at the end, of a period of 3--4 weeks on a reducing diet, during which they lost 5.43 (SD 1.83) kg in weight. The composition of weight loss was also estimated both by energy balance and nitrogen balance during the interval between the two measurements of body composition. 3. The estimates of fat content of the ninetten women at the start of the balance period were 45.63 (SD 14.50) kg by density, 48.07 (SD 13.88) kg by K and 47.09 (SD 13.85) kg by water. The correlation coefficient between the density and K estimate was 0.949, and for the density and water estimate it was 0.971. 4. It is concluded that measurement of density by the new method provides a convenient method for estimating body fatness, and change in fat content, which compares favourably with estimates based on total body water or total body K. However, these methods cannot be used to provide an accurate estimate of the composition of a small weight loss in an individual since deviations up to 4 kg fat occur between fat loss based on change in density and those based on the more reliable (but more tedious) energy balance method."}
{"id": "PMID:476036", "title": "The relationship between protein turnover and energy balance in lean and genetically obese (ob/ob) mice.", "content": "1. Groups of lean and genetically obese (ob/ob) mice were adapted to varying energy intakes and the rates of total protein turnover in liver, gut and kidney were measured. 2. Lean mice gained less weight when fed above maintenance and lost less weight when fed below maintance than obese mice. 3. Hepatic protein turnover (mg/d) was sigmoidally related to digestible energy intake in lean mice but showed no significant changes with dietary intake in obese mice. 4. The changes in protein turnover resulted from changes in both the half-lives of protein synthesis and catabolism and in tissue protein content. 5. In the lean mice, protein turnover in kidney and gut was not significantly changed with increasing energy intake until the highest level was reached. 6. The findings suggest that protein turnover may be an important cycle for the regulation of energy balance in mice and that this cycle is impaired in the genetically obese (ob/ob) mice.", "contents": "The relationship between protein turnover and energy balance in lean and genetically obese (ob/ob) mice. 1. Groups of lean and genetically obese (ob/ob) mice were adapted to varying energy intakes and the rates of total protein turnover in liver, gut and kidney were measured. 2. Lean mice gained less weight when fed above maintenance and lost less weight when fed below maintance than obese mice. 3. Hepatic protein turnover (mg/d) was sigmoidally related to digestible energy intake in lean mice but showed no significant changes with dietary intake in obese mice. 4. The changes in protein turnover resulted from changes in both the half-lives of protein synthesis and catabolism and in tissue protein content. 5. In the lean mice, protein turnover in kidney and gut was not significantly changed with increasing energy intake until the highest level was reached. 6. The findings suggest that protein turnover may be an important cycle for the regulation of energy balance in mice and that this cycle is impaired in the genetically obese (ob/ob) mice."}
{"id": "PMID:476037", "title": "Effects of saponins on bile acids and plasma lipids in the rat.", "content": "1. The effects of feeding isolated saponins on plasma lipid concentrations and on concentrations of biliary and faecal bile acids and neutral sterols were studied in the rat. 2. The animals were given one of four diets, i.e. a standard low-cholesterol synthetic diet, the diet + 10 g saponins/kg, the diet + 10 g cholesterol/kg, the diet + 10 g cholesterol + 10 g saponins/kg. 3. Saponins partially reversed the hypercholesterolaemia caused by the high-cholesterol diet and increased both the rate of bile acid secretion and the faecal excretion of bile acids and neutral sterols. The proportionate contribution of the primary bile acids (particularly chenodeoxycholic) to faecal excretion was also increased by saponins. 4. The results are discussed in relation to the hypothesis that saponins act by inducing the adsorption of bile acids by dietary fibre.", "contents": "Effects of saponins on bile acids and plasma lipids in the rat. 1. The effects of feeding isolated saponins on plasma lipid concentrations and on concentrations of biliary and faecal bile acids and neutral sterols were studied in the rat. 2. The animals were given one of four diets, i.e. a standard low-cholesterol synthetic diet, the diet + 10 g saponins/kg, the diet + 10 g cholesterol/kg, the diet + 10 g cholesterol + 10 g saponins/kg. 3. Saponins partially reversed the hypercholesterolaemia caused by the high-cholesterol diet and increased both the rate of bile acid secretion and the faecal excretion of bile acids and neutral sterols. The proportionate contribution of the primary bile acids (particularly chenodeoxycholic) to faecal excretion was also increased by saponins. 4. The results are discussed in relation to the hypothesis that saponins act by inducing the adsorption of bile acids by dietary fibre."}
{"id": "PMID:476038", "title": "Experiments with the long-term rumen simulation technique (Rusitec); response to supplementation of basal rations.", "content": "1. It is shown that a basal roughage diet can be successfully supplemented with glucose, as long as nitrogen is not limiting. Lack of N depresses the digestibility of the basal ration, results in incomplete fermentation and the increased recoveries of N are consistent with fixation of atmospheric N2. 2. Using a complex, but soluble supplement (whey powder) it is shown that reproducible incremental measurements can be made and that the supplement used gives increases in production of characteristic end-products only (carbon dioxide, methane, acetic and butyric acids). 3. The location of the solid dietary components within the reaction vessel is not important and it is possible to measure changes in a particular component in the presence of others. It is shown that there is sequestration of bacteria and protozoa on the solid digesta.", "contents": "Experiments with the long-term rumen simulation technique (Rusitec); response to supplementation of basal rations. 1. It is shown that a basal roughage diet can be successfully supplemented with glucose, as long as nitrogen is not limiting. Lack of N depresses the digestibility of the basal ration, results in incomplete fermentation and the increased recoveries of N are consistent with fixation of atmospheric N2. 2. Using a complex, but soluble supplement (whey powder) it is shown that reproducible incremental measurements can be made and that the supplement used gives increases in production of characteristic end-products only (carbon dioxide, methane, acetic and butyric acids). 3. The location of the solid dietary components within the reaction vessel is not important and it is possible to measure changes in a particular component in the presence of others. It is shown that there is sequestration of bacteria and protozoa on the solid digesta."}
{"id": "PMID:476039", "title": "Experiments with the longterm rumen simulation technique (Rusitec); use of soluble food and an inert solid matrix.", "content": "1. The role of soluble nutrients and of the solid matrix in rumen fermentation was investigated in some detail, and experiments designed to explore the possibility of using a balanced soluble diet and an inert solid matrix, are described. 2. The use of a balanced soluble substrate as the only source of nutrients in the presence of an inert solid phase in the reaction vessels results in vigorous fermentation but is accompanied by disappearance of protozoa from the effluent. 3. In the absence of digestible solid phase, the rate of fermentation and the fermentation pattern depends mainly on the amount and type of nutrients supplied and to a smaller extent on the dilution rate, the variations being greatest at low dilution rates. 4. The solid matrix in the form of wood shavings or the residue remaining after prolonged digestion of hay could sequestrate micro-organisms and could be used as solid phase, but the defined mixture of soluble substrates used resulted in somewhat abnormal fermentation compared with fermentation obtained with solid food. 5. When the solid food included some hay extract and when the dilution rate was not too great a reduced output of protozoa could be maintained. At high dilution rate the outflow of protozoa was negligible and yet considerable numbers of protozoa were found in the solid matrix and associated liquid. 6. A three-compartment model was developed to describe the flow of liquid and microbial matter within the simplified system.", "contents": "Experiments with the longterm rumen simulation technique (Rusitec); use of soluble food and an inert solid matrix. 1. The role of soluble nutrients and of the solid matrix in rumen fermentation was investigated in some detail, and experiments designed to explore the possibility of using a balanced soluble diet and an inert solid matrix, are described. 2. The use of a balanced soluble substrate as the only source of nutrients in the presence of an inert solid phase in the reaction vessels results in vigorous fermentation but is accompanied by disappearance of protozoa from the effluent. 3. In the absence of digestible solid phase, the rate of fermentation and the fermentation pattern depends mainly on the amount and type of nutrients supplied and to a smaller extent on the dilution rate, the variations being greatest at low dilution rates. 4. The solid matrix in the form of wood shavings or the residue remaining after prolonged digestion of hay could sequestrate micro-organisms and could be used as solid phase, but the defined mixture of soluble substrates used resulted in somewhat abnormal fermentation compared with fermentation obtained with solid food. 5. When the solid food included some hay extract and when the dilution rate was not too great a reduced output of protozoa could be maintained. At high dilution rate the outflow of protozoa was negligible and yet considerable numbers of protozoa were found in the solid matrix and associated liquid. 6. A three-compartment model was developed to describe the flow of liquid and microbial matter within the simplified system."}
{"id": "PMID:476041", "title": "Effects of the protracted feeding of copper sulphate-supplemented diets to laying, domestic fowl on egg production and on specific tissues, with special reference to mineral content.", "content": "1. Two experiments are reported. In both experiments a cereal-based diet containing 5 mg copper/kg was fed to two breeds of laying hens for 336 d. In Expt 1 four other groups were given this diet with the addition of CuSO4.5H2O to give added levels of 200, 400, 600 and 800 mg Cu/kg diet. In Expt 2 the levels of added dietary Cu used were 100, 200, 300 and 400 mg/kg. 2. In Expt 1 records were kept of food intake, water intake, body-weight and egg production for eight 28 d periods and body-weight and egg number only were recorded for the full twelve periods. In Expt 2 full records, excluding water intake, were taken for all twelve periods. 3. Food and water intake showed a quadratic response to level of added dietary Cu, being enhanced at lower levels and depressed at higher levels of addition. 4. There was a quadratic response of total egg weight, mean egg weight and egg number to added dietary Cu. In Expt 1 egg number was maximum at 235 mg added Cu/kg diet for Warren Studler SSL (breed 1) and at 170 mg added Cu/kg diet for Shaver 288 (breed 2). In Expt 2 no breed effect occurred, the maximum egg number being calculated to occur at 176 mg added Cu/kg diet. 5. Depression of body-weight gain occurred at high levels of Cu addition. The depression of liver and oviduct weight found at high levels of addition appeared to be directly related to body-weight. A marked amount of feather loss also occurred at a high inclusion of CuSO4 in the diet. 6. The reproductive systems of the hens did not appear to be adversely affected at the levels of additive used. Gross and microscopic examination of specific tissues revealed no pathological effects although gizzard and intestinal weights were increased and caecal weight decreased by high levels of added Cu. THose aspects of the blood chemistry examined did not reveal any consistent effect between the two experiments. 7. The liver Cu analyses indicate that between 600 and 800 mg added Cu/kg diet the liver Cu concentration rises sharply. Both liver Fe and Zn concentrations showed a positive linear response to added dietary Cu. In the kidney Cu and Zn concentrations were increased but only to a limited extent, while the concentration of Fe was unaffected.", "contents": "Effects of the protracted feeding of copper sulphate-supplemented diets to laying, domestic fowl on egg production and on specific tissues, with special reference to mineral content. 1. Two experiments are reported. In both experiments a cereal-based diet containing 5 mg copper/kg was fed to two breeds of laying hens for 336 d. In Expt 1 four other groups were given this diet with the addition of CuSO4.5H2O to give added levels of 200, 400, 600 and 800 mg Cu/kg diet. In Expt 2 the levels of added dietary Cu used were 100, 200, 300 and 400 mg/kg. 2. In Expt 1 records were kept of food intake, water intake, body-weight and egg production for eight 28 d periods and body-weight and egg number only were recorded for the full twelve periods. In Expt 2 full records, excluding water intake, were taken for all twelve periods. 3. Food and water intake showed a quadratic response to level of added dietary Cu, being enhanced at lower levels and depressed at higher levels of addition. 4. There was a quadratic response of total egg weight, mean egg weight and egg number to added dietary Cu. In Expt 1 egg number was maximum at 235 mg added Cu/kg diet for Warren Studler SSL (breed 1) and at 170 mg added Cu/kg diet for Shaver 288 (breed 2). In Expt 2 no breed effect occurred, the maximum egg number being calculated to occur at 176 mg added Cu/kg diet. 5. Depression of body-weight gain occurred at high levels of Cu addition. The depression of liver and oviduct weight found at high levels of addition appeared to be directly related to body-weight. A marked amount of feather loss also occurred at a high inclusion of CuSO4 in the diet. 6. The reproductive systems of the hens did not appear to be adversely affected at the levels of additive used. Gross and microscopic examination of specific tissues revealed no pathological effects although gizzard and intestinal weights were increased and caecal weight decreased by high levels of added Cu. THose aspects of the blood chemistry examined did not reveal any consistent effect between the two experiments. 7. The liver Cu analyses indicate that between 600 and 800 mg added Cu/kg diet the liver Cu concentration rises sharply. Both liver Fe and Zn concentrations showed a positive linear response to added dietary Cu. In the kidney Cu and Zn concentrations were increased but only to a limited extent, while the concentration of Fe was unaffected."}
{"id": "PMID:476042", "title": "Studies on digestion and absorption in the intestines of growing pigs. 7. Measurements of the flow of total carbohydrate, total reducing substances and glucose.", "content": "1. Seventeen pigs were fitted with single re-entrant cannulas in either the duodenum (posterior to the entry of the bile and pancreatic ducts), the mid-jejunum, or the terminal ileum. A further twenty-four pigs were used in a conventional digestibility trial. 2. Three diets were used: these contained barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF) ; starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut meal (diet SSG) or casein (diet SSC). 3. The quantities of total carbohydrate (TC), total reducing substances (TRS) and glucose (G) passing through the re-entrant cannulas and excreted in the faeces in 24 h were measured. These were used to determine the net absorption of the carbohydrate fractions in the different regions of the intestine. 4. The small intestine was the principal site of absorption of TC, TRS and G, but there were differences between the diets in the quantities of each of these carbohydrate fractions that were absorbed in the different regions of the small intestine studied. 5. The quantities of TRS and G in solution were very low for all diets at all sites, indicating that the rate of absorption of the products of hydrolysis kept pace with their rate of formation.", "contents": "Studies on digestion and absorption in the intestines of growing pigs. 7. Measurements of the flow of total carbohydrate, total reducing substances and glucose. 1. Seventeen pigs were fitted with single re-entrant cannulas in either the duodenum (posterior to the entry of the bile and pancreatic ducts), the mid-jejunum, or the terminal ileum. A further twenty-four pigs were used in a conventional digestibility trial. 2. Three diets were used: these contained barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF) ; starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut meal (diet SSG) or casein (diet SSC). 3. The quantities of total carbohydrate (TC), total reducing substances (TRS) and glucose (G) passing through the re-entrant cannulas and excreted in the faeces in 24 h were measured. These were used to determine the net absorption of the carbohydrate fractions in the different regions of the intestine. 4. The small intestine was the principal site of absorption of TC, TRS and G, but there were differences between the diets in the quantities of each of these carbohydrate fractions that were absorbed in the different regions of the small intestine studied. 5. The quantities of TRS and G in solution were very low for all diets at all sites, indicating that the rate of absorption of the products of hydrolysis kept pace with their rate of formation."}
{"id": "PMID:476043", "title": "Studies on digestion and absorption in the intestines of growing pigs. 8. Measurements of the flow of total lipid, acid-detergent fibre and volatile fatty acids.", "content": "1. Seventeen pigs fitted with single re-entrant cannulas in either the duodenum (posterior to the point of entry of the bile and pancreatic ducts), the mid-jejunum, or the terminal ileum, and a further twenty-four non-cannulated pigs were used. 2. Three diets were used: these contained barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF); starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut meal (diet SSG) or casein (diet SSC). 3. The quantities of total lipid (TL), acid-detergent fibre (ADF) and volatile fatty acids (VFA) passing through the re-entrant cannulas and excreted in the faeces in 24 h were measured. These were used to determine the net absorption (or synthesis or secretion) in the different regions of the intestine. 4. There was substantial secretion of TL into the proximal small intestine and major absorption from the distal portion for all three diets. In the large intestine there was little or no further net absorption for diets SSG and SSC, but significant synthesis or secretion for diet BWF. 5. Fibre digestion occurred in both the small and large intestines and the apparent absorption of ADF in the different regions studied was influenced by diet. 6. There were detectable amounts of VFA at all sites but only at the ileum and in the faeces could they be accurately measured. Diet did not affect the molar proportions of acetate, propionate and butyrate in ileal digesta, but did have an effect on these VFA in the faeces.", "contents": "Studies on digestion and absorption in the intestines of growing pigs. 8. Measurements of the flow of total lipid, acid-detergent fibre and volatile fatty acids. 1. Seventeen pigs fitted with single re-entrant cannulas in either the duodenum (posterior to the point of entry of the bile and pancreatic ducts), the mid-jejunum, or the terminal ileum, and a further twenty-four non-cannulated pigs were used. 2. Three diets were used: these contained barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF); starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut meal (diet SSG) or casein (diet SSC). 3. The quantities of total lipid (TL), acid-detergent fibre (ADF) and volatile fatty acids (VFA) passing through the re-entrant cannulas and excreted in the faeces in 24 h were measured. These were used to determine the net absorption (or synthesis or secretion) in the different regions of the intestine. 4. There was substantial secretion of TL into the proximal small intestine and major absorption from the distal portion for all three diets. In the large intestine there was little or no further net absorption for diets SSG and SSC, but significant synthesis or secretion for diet BWF. 5. Fibre digestion occurred in both the small and large intestines and the apparent absorption of ADF in the different regions studied was influenced by diet. 6. There were detectable amounts of VFA at all sites but only at the ileum and in the faeces could they be accurately measured. Diet did not affect the molar proportions of acetate, propionate and butyrate in ileal digesta, but did have an effect on these VFA in the faeces."}
{"id": "PMID:476044", "title": "Protein quality in cereals and pulses. 2. Influence of polyethyleneglycol on the nutritional availability of methionine in sorghum (Sorghum vulgar Pers.), field beans (Vicia faba L.) and barley.", "content": "1. Polyethyleneglycol (PEG 4000) was examined for its influence on relative nutritional value (RNV) and available methionine in sorghum (Sorghum vulgare Pers.), field beans (Vicia faba L.) and barley, as measured microbiologically with Streptococcus zymogenes. The results were assessed in relation to the content of tannins in the test samples. 2. In grain of hybrid sorghum the RNV averaged 87 (range 79--92) for six low-tannin varieties and 41 (30--53) for eleven high-tannin varieties. The corresponding available methionine values averaged 17.0 (15.7--18.9) and 8.9 (6.7--11.0) g/kg protein. Addition of PEG 4000 to the test samples increased the average RNV of the high-tannin varieties from 41 to 78, and the average available methionine content from 8.9 to 16.2 g/kg protein. 3. With seed of ten coloured flowered varieties of field beans, treatment with PEG gave a small but consistent increase in the available methionine content, which resulted from the inactivation of tannins in the testa. 4. In twenty-three samples of barley grain, treatment with PEG had no effect on the values obtained for available methionine. 5. Treatment of high-tannin sorghum grain with ammonia has been reported to inactivate the tannins and increase the nutritional value for rats and chicks. This finding was confirmed. The present study showed that ammonia and PEG 4000 were equally effective in enhancing the nutritional quality as measured in the microbiological tests.", "contents": "Protein quality in cereals and pulses. 2. Influence of polyethyleneglycol on the nutritional availability of methionine in sorghum (Sorghum vulgar Pers.), field beans (Vicia faba L.) and barley. 1. Polyethyleneglycol (PEG 4000) was examined for its influence on relative nutritional value (RNV) and available methionine in sorghum (Sorghum vulgare Pers.), field beans (Vicia faba L.) and barley, as measured microbiologically with Streptococcus zymogenes. The results were assessed in relation to the content of tannins in the test samples. 2. In grain of hybrid sorghum the RNV averaged 87 (range 79--92) for six low-tannin varieties and 41 (30--53) for eleven high-tannin varieties. The corresponding available methionine values averaged 17.0 (15.7--18.9) and 8.9 (6.7--11.0) g/kg protein. Addition of PEG 4000 to the test samples increased the average RNV of the high-tannin varieties from 41 to 78, and the average available methionine content from 8.9 to 16.2 g/kg protein. 3. With seed of ten coloured flowered varieties of field beans, treatment with PEG gave a small but consistent increase in the available methionine content, which resulted from the inactivation of tannins in the testa. 4. In twenty-three samples of barley grain, treatment with PEG had no effect on the values obtained for available methionine. 5. Treatment of high-tannin sorghum grain with ammonia has been reported to inactivate the tannins and increase the nutritional value for rats and chicks. This finding was confirmed. The present study showed that ammonia and PEG 4000 were equally effective in enhancing the nutritional quality as measured in the microbiological tests."}
{"id": "PMID:476045", "title": "Protein quality in cereals and pulses. 3. Bioassays with rats and chickens on sorghum (Sorghum vulgare Pers.), barley and field beans (Vicia faba L.). Influence of polyethylene glycol on digestibility on the protein in high-tannin grain.", "content": "1. Two preceding papers in this series describe the application of microbiological and other in vitro tests in the evaluation of sorghum (Sorghum vulgare Pers.), field beans (Vicia faba L.) and barley, and in assessing the influence of polyethylene glycol (PEG 4000) on the nutritional availability of the methionine. The present paper gives for comparison the results of bioassays on some of the same test samples. Net protein utilization (NPU) in rats was measured by the nitrogen balance method, and N digestibility in chickens by the ileal analysis procedure. 2. In rat tests on sorghum, N in grain of high-tannin varieties was poorly digested. Supplementation of the test diets with 0.1 g PEG 4000/g protein gave a large improvemnet, which was partly offset by an apparent decrease in biological value (BV). With chickens N digestibility was even lower, and was similarly improved with PEG 4000. Treatment of high-tannin grain with ammonia solution was also effective in improving N digestibility. 3. With low-tannin sorghum the amino acid digestibilities were uniformly high and were not affected by addition of PEG to the test diet. With high-tannin sorghums they were low and less uniform, and were much improved by PEG 4000. 4. With field beans, the influence of the seed-coat tannin on protein utilization was much less pronounced than with sorghum. In chickens there was a significant effect (P less than 0.05) of PEG 4000 on N idgestibility in a high-tannin variety. With rats the effect was smaller and not significant. 5. In four samples of barley. N digestibility was high (0.87--0.96) and was not further improved by PEG 4000. The BV of a high-lysine cultivar proved marginally inferior to that of a normal variety. Possible reasons for this are discussed. 6. Over all, the results were closely consistent with those from microbiological tests with Streptococcus zymogenes.", "contents": "Protein quality in cereals and pulses. 3. Bioassays with rats and chickens on sorghum (Sorghum vulgare Pers.), barley and field beans (Vicia faba L.). Influence of polyethylene glycol on digestibility on the protein in high-tannin grain. 1. Two preceding papers in this series describe the application of microbiological and other in vitro tests in the evaluation of sorghum (Sorghum vulgare Pers.), field beans (Vicia faba L.) and barley, and in assessing the influence of polyethylene glycol (PEG 4000) on the nutritional availability of the methionine. The present paper gives for comparison the results of bioassays on some of the same test samples. Net protein utilization (NPU) in rats was measured by the nitrogen balance method, and N digestibility in chickens by the ileal analysis procedure. 2. In rat tests on sorghum, N in grain of high-tannin varieties was poorly digested. Supplementation of the test diets with 0.1 g PEG 4000/g protein gave a large improvemnet, which was partly offset by an apparent decrease in biological value (BV). With chickens N digestibility was even lower, and was similarly improved with PEG 4000. Treatment of high-tannin grain with ammonia solution was also effective in improving N digestibility. 3. With low-tannin sorghum the amino acid digestibilities were uniformly high and were not affected by addition of PEG to the test diet. With high-tannin sorghums they were low and less uniform, and were much improved by PEG 4000. 4. With field beans, the influence of the seed-coat tannin on protein utilization was much less pronounced than with sorghum. In chickens there was a significant effect (P less than 0.05) of PEG 4000 on N idgestibility in a high-tannin variety. With rats the effect was smaller and not significant. 5. In four samples of barley. N digestibility was high (0.87--0.96) and was not further improved by PEG 4000. The BV of a high-lysine cultivar proved marginally inferior to that of a normal variety. Possible reasons for this are discussed. 6. Over all, the results were closely consistent with those from microbiological tests with Streptococcus zymogenes."}
{"id": "PMID:476047", "title": "Histone gene switch in the sea urchin embryo. Identification of late embryonic histone messenger ribonucleic acids and the control of their synthesis.", "content": "During embryogenesis in the sea urchin Strongylocentrotus purpuratus, there is a shift from one histone mRNA population to another. The early and late embryonic histone mRNAs, previously shown to differ considerably in sequence from each other by hybrid melting studies, are shown here to differ also in electrophoretic mobility on polyacrylamide gels as the positions of the early and late mRNAs are completely noncoincident. The various species of both early and late samples are identified as particular histone mRNAs by hybridization to cloned histone DNAs containing part of the early-type repeat unit or to restriction enzyme fragments derived from these unit. Four bands in the early mRNA sample are identified as H1, H3, H2A \" H2B, and H4 mRNA while at least 10 bands can be seen in the late mRNA preparation with unambiguous identification of H1, H2B, and H4 mRNAs. A cluster of late species is shown to contain both H3 and H2A mRNA. When a polysomal RNA preparation from the 26-h embryo is hybridized to the histone DNA, eluted, and then translated in vitro in a wheat germ system, the histone products migrate in the position of late histones when subjected to electrophoresis on Triton X-urea gels. Using DNA which contains genes for H2A + H3 or H2A alone, we demonstrate the specificity of the early-type DNA probes for these two late histones. Therefore, by hybridization of newly synthesized RNAs and translation of the total polysomal RNA present in the late embryo, it is shown that mRNAs for all five histone classes may cross-react with the cloned early-type DNA. The hybrids formed, however, are much less stable than those formed with the early histone mRNA. In vitro translation of total cytoplasmic RNA from various embryonic stages indicates that transition between the two classes occurs during most of the blastula period.", "contents": "Histone gene switch in the sea urchin embryo. Identification of late embryonic histone messenger ribonucleic acids and the control of their synthesis. During embryogenesis in the sea urchin Strongylocentrotus purpuratus, there is a shift from one histone mRNA population to another. The early and late embryonic histone mRNAs, previously shown to differ considerably in sequence from each other by hybrid melting studies, are shown here to differ also in electrophoretic mobility on polyacrylamide gels as the positions of the early and late mRNAs are completely noncoincident. The various species of both early and late samples are identified as particular histone mRNAs by hybridization to cloned histone DNAs containing part of the early-type repeat unit or to restriction enzyme fragments derived from these unit. Four bands in the early mRNA sample are identified as H1, H3, H2A \" H2B, and H4 mRNA while at least 10 bands can be seen in the late mRNA preparation with unambiguous identification of H1, H2B, and H4 mRNAs. A cluster of late species is shown to contain both H3 and H2A mRNA. When a polysomal RNA preparation from the 26-h embryo is hybridized to the histone DNA, eluted, and then translated in vitro in a wheat germ system, the histone products migrate in the position of late histones when subjected to electrophoresis on Triton X-urea gels. Using DNA which contains genes for H2A + H3 or H2A alone, we demonstrate the specificity of the early-type DNA probes for these two late histones. Therefore, by hybridization of newly synthesized RNAs and translation of the total polysomal RNA present in the late embryo, it is shown that mRNAs for all five histone classes may cross-react with the cloned early-type DNA. The hybrids formed, however, are much less stable than those formed with the early histone mRNA. In vitro translation of total cytoplasmic RNA from various embryonic stages indicates that transition between the two classes occurs during most of the blastula period."}
{"id": "PMID:476048", "title": "Hemocyanin of Octopus vulgaris. The molecular weight of the minimal functional subunit in 3 M urea.", "content": "In the presence of 3 M urea Octopus vulgaris (Mollusca) hemocyanin dissociates completely, giving a single functional component which reassociates in the original aggregate after removal of urea. The molecular weight of this subunit has been determined by gel filtration, by viscosity measurements, and by ultracentrifugation. The values obtrained with the different methods range from 247,000 to 262,000. Electron microscopy shows that the reassociation of the functional subunit after removal of urea is complete and gives the typical cylindrical structure of the native protein. This component is the minimal functional subunit which can be obtained from Octopus hemocyanin without splitting covalent bonds. It is suggested that this component is made by five protomers (50,000 daltons) containing one oxygen binding site each bound covalently through, perhaps, the carbohydrate moieties of the protein.", "contents": "Hemocyanin of Octopus vulgaris. The molecular weight of the minimal functional subunit in 3 M urea. In the presence of 3 M urea Octopus vulgaris (Mollusca) hemocyanin dissociates completely, giving a single functional component which reassociates in the original aggregate after removal of urea. The molecular weight of this subunit has been determined by gel filtration, by viscosity measurements, and by ultracentrifugation. The values obtrained with the different methods range from 247,000 to 262,000. Electron microscopy shows that the reassociation of the functional subunit after removal of urea is complete and gives the typical cylindrical structure of the native protein. This component is the minimal functional subunit which can be obtained from Octopus hemocyanin without splitting covalent bonds. It is suggested that this component is made by five protomers (50,000 daltons) containing one oxygen binding site each bound covalently through, perhaps, the carbohydrate moieties of the protein."}
{"id": "PMID:476051", "title": "Synthesis of 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid and detection of skin fibroblast neuraminidase in normal humans and in sialidosis.", "content": "A procedure for the synthesis of the fluorogenic substrate analogue 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid for the human acid neuraminidase has been developed. The substrate was employed for the characterization of the enzyme in sonicates of cultured human skin fibroblasts and for enzymatic detection of the neuraminidase deficiency in the neurological storage disorder, sialidosis. Synthesis was accomplished by reacting 2-deoxy-2-chloro-4,7,8,9-tetra-O-acetyl-N-acetylneuraminic acid methyl ester with the sodium salt of 4-methylumbelliferone in acetonitrile at room temperature. The coupled product was purified on silicic acid chromatography, followed by base-catalyzed removal of the O-acetyl and methoxy blocking groups, and with additional purification of the hydrolyzed product on silicic acid. The overall yield, based on N-acetylneuraminic acid, was 37%. Under linear assay conditions, at pH 4.3, the apparent maximal velocities (nmol (mg of protein)-1 h-1) for normal fibroblasts were 58--115, 0.2--1.8 for sialidosis fibroblasts, and 28--38 for obligate heterozygotes. The apparent Km for normals was 0.13 mM.", "contents": "Synthesis of 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid and detection of skin fibroblast neuraminidase in normal humans and in sialidosis. A procedure for the synthesis of the fluorogenic substrate analogue 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid for the human acid neuraminidase has been developed. The substrate was employed for the characterization of the enzyme in sonicates of cultured human skin fibroblasts and for enzymatic detection of the neuraminidase deficiency in the neurological storage disorder, sialidosis. Synthesis was accomplished by reacting 2-deoxy-2-chloro-4,7,8,9-tetra-O-acetyl-N-acetylneuraminic acid methyl ester with the sodium salt of 4-methylumbelliferone in acetonitrile at room temperature. The coupled product was purified on silicic acid chromatography, followed by base-catalyzed removal of the O-acetyl and methoxy blocking groups, and with additional purification of the hydrolyzed product on silicic acid. The overall yield, based on N-acetylneuraminic acid, was 37%. Under linear assay conditions, at pH 4.3, the apparent maximal velocities (nmol (mg of protein)-1 h-1) for normal fibroblasts were 58--115, 0.2--1.8 for sialidosis fibroblasts, and 28--38 for obligate heterozygotes. The apparent Km for normals was 0.13 mM."}
{"id": "PMID:476052", "title": "Thymidylate synthetase. Catalysis of dehalogenation of 5-bromo- and 5-iodo-2'-deoxyuridylate.", "content": "Tymidylate synthetase catalyzes the facile dehalogenation of 5-bromo-2'-deoxyuridylate (BrdUMP) and 5-iodo-2'-deoxyuridylate )IdUMP) to give 2'-deoxyuridylate (dUMP), the natural substrate of the enzyme. The reaction does not require folate cofactors and stoichiometrically consumes 2 equiv of thiol. In addition to dUMP, a minor product is formed during the debromination of BrdUMP which has been identified as a 5-alkylthio derivative formed by displacement of bromide ion by thiolate. The reaction has been found to proceed with a substantial alpha-secondary inverse tritium isotope effect (kT/kH = 1.212--1.258) with [2-14C,6-3H]-BrdUMP as the substrate. Similarly, an inverse tritiumisotope effect of 1.18 was observed in the nonenzymatic chemical counterpart of this reaction, the cysteine-promoted dehalogenation of [2-14C,6-3H]-5-bromo-2'-deoxyuridine. Previous evidence for the mechanism of action of this enzyme has rested largely on chemical model studies and on information obtained from its stoichiometric interaction with the inhibitor 5-fluoro-2'-deoxyuridylate. The magnitude of the secondary isotope effect during the enzymatic dehalogenation described here provides direct proof for nucleophilic catalysis and formation of 5,6-dihydroprimidine intermediates in a reaction catalyzed by thymidylate synthetase.", "contents": "Thymidylate synthetase. Catalysis of dehalogenation of 5-bromo- and 5-iodo-2'-deoxyuridylate. Tymidylate synthetase catalyzes the facile dehalogenation of 5-bromo-2'-deoxyuridylate (BrdUMP) and 5-iodo-2'-deoxyuridylate )IdUMP) to give 2'-deoxyuridylate (dUMP), the natural substrate of the enzyme. The reaction does not require folate cofactors and stoichiometrically consumes 2 equiv of thiol. In addition to dUMP, a minor product is formed during the debromination of BrdUMP which has been identified as a 5-alkylthio derivative formed by displacement of bromide ion by thiolate. The reaction has been found to proceed with a substantial alpha-secondary inverse tritium isotope effect (kT/kH = 1.212--1.258) with [2-14C,6-3H]-BrdUMP as the substrate. Similarly, an inverse tritiumisotope effect of 1.18 was observed in the nonenzymatic chemical counterpart of this reaction, the cysteine-promoted dehalogenation of [2-14C,6-3H]-5-bromo-2'-deoxyuridine. Previous evidence for the mechanism of action of this enzyme has rested largely on chemical model studies and on information obtained from its stoichiometric interaction with the inhibitor 5-fluoro-2'-deoxyuridylate. The magnitude of the secondary isotope effect during the enzymatic dehalogenation described here provides direct proof for nucleophilic catalysis and formation of 5,6-dihydroprimidine intermediates in a reaction catalyzed by thymidylate synthetase."}
{"id": "PMID:476055", "title": "Dialdehydes derived from adenine nucleosides as substrates and inhibitors of adenosine aminohydrolase.", "content": "A series of nucleoside dialdehydes have been obtained as powders after treatment of various adenine nucleosides with paraperiodic acid. Thus, oxidation gave dialdehydes derived from adenosine (1), 9-alpha-D-mannopyranosyladenine (2), 9-(5-deoxy-alpha-D-arabinofuranosyl)adenine (3), 9-alpha-L-rhamnopyranosyladenine (4), 9-beta-L-fucopyranosyladenine (5), 9-beta-D-fucopyranosyladenine (6), 9-alpha-D-arabinopyranosyladenine (7), 9-beta-D-ribopyranosyladenine (8), and 9-(5-deoxy-beta-D-erythro-pent-4-enofuranosyl)adenine (9). Nucleoside dialdehydes 1-3 and 6-8 were weak substrates for adenosine aminohydrolase from calf intestinal mucosa. Dialdehyde 8 had the strongest affinity, but 1 had the highest Vmax. All of the dialdehydes except 5 were inhibitors of the enzyme. The best inhibitors were 9 (Ki = 4 microM) and 4 (ki = 28 microM), and neither were substrates. The inhibitors did not exhibit time-dependent inhibition and did not appear to form covalent bonds with the protein. The data strongly suggest that the active form of the dialdehydes is as the open-chain dihydrates. The alcohol obtained by reduction of 9 (compound 10) was the strongest inhibitor (Ki = 0.9 microM among the related alcohols and the nucleoside dialdehydes.", "contents": "Dialdehydes derived from adenine nucleosides as substrates and inhibitors of adenosine aminohydrolase. A series of nucleoside dialdehydes have been obtained as powders after treatment of various adenine nucleosides with paraperiodic acid. Thus, oxidation gave dialdehydes derived from adenosine (1), 9-alpha-D-mannopyranosyladenine (2), 9-(5-deoxy-alpha-D-arabinofuranosyl)adenine (3), 9-alpha-L-rhamnopyranosyladenine (4), 9-beta-L-fucopyranosyladenine (5), 9-beta-D-fucopyranosyladenine (6), 9-alpha-D-arabinopyranosyladenine (7), 9-beta-D-ribopyranosyladenine (8), and 9-(5-deoxy-beta-D-erythro-pent-4-enofuranosyl)adenine (9). Nucleoside dialdehydes 1-3 and 6-8 were weak substrates for adenosine aminohydrolase from calf intestinal mucosa. Dialdehyde 8 had the strongest affinity, but 1 had the highest Vmax. All of the dialdehydes except 5 were inhibitors of the enzyme. The best inhibitors were 9 (Ki = 4 microM) and 4 (ki = 28 microM), and neither were substrates. The inhibitors did not exhibit time-dependent inhibition and did not appear to form covalent bonds with the protein. The data strongly suggest that the active form of the dialdehydes is as the open-chain dihydrates. The alcohol obtained by reduction of 9 (compound 10) was the strongest inhibitor (Ki = 0.9 microM among the related alcohols and the nucleoside dialdehydes."}
{"id": "PMID:476056", "title": "Amino acid catalyzed condensation of purines and pyrimidines with 2-deoxyribose.", "content": "Mild heating of aqueous mixtures containing 2-deoxyribose, amino compounds, and purines or pyrimidines produces derivatives of the purines and pyrimidines in high yield. Among the major products formed are 2,3-dideoxy-3-(1'-pyrimidino)pentose and 2,3-dideoxy-3-(9'-purino)pentose. The mechanism of the reaction includes amine-catalzyed dehydration of the alpha, beta positions of the sugar followed by addition of the purine or pyrimidine to the double bond. Rapid addition of purines and pyrimidines to alpha, beta-unsaturated carbonyl compounds (such as acrolin) is a general phenomenon which does not require an amine catalyst. While multiple derivatization of the purines will take place, the N-9 derivative is formed first.", "contents": "Amino acid catalyzed condensation of purines and pyrimidines with 2-deoxyribose. Mild heating of aqueous mixtures containing 2-deoxyribose, amino compounds, and purines or pyrimidines produces derivatives of the purines and pyrimidines in high yield. Among the major products formed are 2,3-dideoxy-3-(1'-pyrimidino)pentose and 2,3-dideoxy-3-(9'-purino)pentose. The mechanism of the reaction includes amine-catalzyed dehydration of the alpha, beta positions of the sugar followed by addition of the purine or pyrimidine to the double bond. Rapid addition of purines and pyrimidines to alpha, beta-unsaturated carbonyl compounds (such as acrolin) is a general phenomenon which does not require an amine catalyst. While multiple derivatization of the purines will take place, the N-9 derivative is formed first."}
{"id": "PMID:476059", "title": "Influence of hydrogen bonding in DNA and polynucleotides on reaction of nitrogens and oxygens toward ethylnitrosourea.", "content": "The reactivity of ethylnitrosourea toward hydrogen-bonded sites in double-stranded DNA or oly(rA).poly(rU) was compared with those sites in single-stranded DNA, RNA, or poly(rA). Alkylation of the N-1 of A in poly(rA).poly(rU) was almost suppressed at 5 degrees C but could be markedly increased by raining the reaction temperature to 25 degrees C, well below the Tm of 56 degrees C. In contrast, the N-7 and N-6 of A, which are not hydrogen bonded, reacted to the same extent at temperatures ranging from 5 to 65 degrees C. The extent of reaction at the N-3 of A varied inversely with the reactivity of the N-1 of A, indicating that of these two nitrogens the N-1 of A is the most reactive. The proportion of reaction at the various nitrogens in poly(rA) was not affected by temperature. Hydrogen-bonded oxygens in double-stranded DNA are the O-6 of G, the O-4 of T, and the O2 of C. All are equally reactive at 5, 25, and 51 degrees C. It is concluded that the observed temperature independence is due to these oxygens having an electron pair not involved in hydrogen bonding and, thus, available for reaction. In contrast, the electron pair of the N-1 of A (or the N-3 of C) is involved in hydrogen bonding, and the extent of their reactivity is dependent on thermal fluctuation providing transiently open base pairs at temperatures far below the Tm.", "contents": "Influence of hydrogen bonding in DNA and polynucleotides on reaction of nitrogens and oxygens toward ethylnitrosourea. The reactivity of ethylnitrosourea toward hydrogen-bonded sites in double-stranded DNA or oly(rA).poly(rU) was compared with those sites in single-stranded DNA, RNA, or poly(rA). Alkylation of the N-1 of A in poly(rA).poly(rU) was almost suppressed at 5 degrees C but could be markedly increased by raining the reaction temperature to 25 degrees C, well below the Tm of 56 degrees C. In contrast, the N-7 and N-6 of A, which are not hydrogen bonded, reacted to the same extent at temperatures ranging from 5 to 65 degrees C. The extent of reaction at the N-3 of A varied inversely with the reactivity of the N-1 of A, indicating that of these two nitrogens the N-1 of A is the most reactive. The proportion of reaction at the various nitrogens in poly(rA) was not affected by temperature. Hydrogen-bonded oxygens in double-stranded DNA are the O-6 of G, the O-4 of T, and the O2 of C. All are equally reactive at 5, 25, and 51 degrees C. It is concluded that the observed temperature independence is due to these oxygens having an electron pair not involved in hydrogen bonding and, thus, available for reaction. In contrast, the electron pair of the N-1 of A (or the N-3 of C) is involved in hydrogen bonding, and the extent of their reactivity is dependent on thermal fluctuation providing transiently open base pairs at temperatures far below the Tm."}
{"id": "PMID:476060", "title": "Determination of the size of rat ribosomal deoxyribonucleic acid repeating units by electron microscopy.", "content": "The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtained by this affinity chromatography procedure. This degree of enrichment made it possible to visualize these R loop containing molecules in the electron microscope and, thus, to obtain a map of the transcribed and spacer regions of rat rDNA. Eleven of the molecules that were observed contained either 3 or 4 R loops, or else 2 R loops separated by a long spacer. Thus, these molecules provided direct information in regard to the length of rat rDNA repeating units. The mean length of the repeating units was 37.2 kbp with a standard deviation of 1.3 kbp. Within the errors of the measurements, these could all represent repeating units of exactly the same length, although a certain degree of length heterogeneity, possibly up to 4 or 5 kbp, cannot be ruled out by the data. If significantly longer or shorter rDNA repeating units exist in the rat genome, they are probably much less common than the 37.2 kbp unit. These electron microscopic measurements provide the most definitive data yet available on the size of the repeating units of mammalian rRNA genes.", "contents": "Determination of the size of rat ribosomal deoxyribonucleic acid repeating units by electron microscopy. The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtained by this affinity chromatography procedure. This degree of enrichment made it possible to visualize these R loop containing molecules in the electron microscope and, thus, to obtain a map of the transcribed and spacer regions of rat rDNA. Eleven of the molecules that were observed contained either 3 or 4 R loops, or else 2 R loops separated by a long spacer. Thus, these molecules provided direct information in regard to the length of rat rDNA repeating units. The mean length of the repeating units was 37.2 kbp with a standard deviation of 1.3 kbp. Within the errors of the measurements, these could all represent repeating units of exactly the same length, although a certain degree of length heterogeneity, possibly up to 4 or 5 kbp, cannot be ruled out by the data. If significantly longer or shorter rDNA repeating units exist in the rat genome, they are probably much less common than the 37.2 kbp unit. These electron microscopic measurements provide the most definitive data yet available on the size of the repeating units of mammalian rRNA genes."}
{"id": "PMID:476061", "title": "Nucleosome reconstitution: effect of DNA length on nuclesome structure.", "content": "Core histones (H2A, H2B, H3, and H4) are reconstituted by salt gradient dialysis with DNA molecules ranging in length from 177 bp down to 50 bp. While reconstituted particles containing 125 bp are very similar to native particles, those particles containing a single piece of shorter DNA tend to aggregate. The aggregation depends on the ionic strength and DNA length. The DNA placement on the histone core is not random as determined by pancreatic DNase I digestions of particles containing 32P 5'-end-labeled DNA. Rather, it is found that all DNA molecules, up to 161 bp in length, reassociate with core histones in such a way as to produce defined patterns of DNase I cutting with respect to the 5' ends. Particles were made that contained two pieces of 65-bp DNA. These particles are very similar to native particles under most conditions but tended to dissociation results in the production of two half-nucleosomes (hemisones).", "contents": "Nucleosome reconstitution: effect of DNA length on nuclesome structure. Core histones (H2A, H2B, H3, and H4) are reconstituted by salt gradient dialysis with DNA molecules ranging in length from 177 bp down to 50 bp. While reconstituted particles containing 125 bp are very similar to native particles, those particles containing a single piece of shorter DNA tend to aggregate. The aggregation depends on the ionic strength and DNA length. The DNA placement on the histone core is not random as determined by pancreatic DNase I digestions of particles containing 32P 5'-end-labeled DNA. Rather, it is found that all DNA molecules, up to 161 bp in length, reassociate with core histones in such a way as to produce defined patterns of DNase I cutting with respect to the 5' ends. Particles were made that contained two pieces of 65-bp DNA. These particles are very similar to native particles under most conditions but tended to dissociation results in the production of two half-nucleosomes (hemisones)."}
{"id": "PMID:476062", "title": "Resonance Raman study of flavins and the flavoprotein fatty acyl coenzyme A dehydrogenase.", "content": "The resonance Raman (RR) spectra of FMN, FAD, FAD in D2O, and 7,8-dimethyl-1, 10-ethyleneisoalloxazinium perchlorate have been obtained by employing KI as a collisional fluorescence-quenching agent. The spectra are very similar to those obtained recently by using the CARS technique to eliminate fluorescence. Spectra have also been obtained for several species in which flavin is known to fluoresce only weakly. We report RR spectra of protonated FMN, FMN semiquinone cation, the general fatty acyl-CoA dehydrogenase, and two \"charge-transfer\" complexes of fatty acyl-CoA dehydrogenase. Tentative assignment of several vibrational bands can be made on the basis of our flavin spectra. RR spectra of fatty acyl-CoA and its complexes are consistent with the previous hypothesis that visible spectral shifts observed during formation of acetoacetyl-CoA and crotonyl-CoA complexes of fatty acyl-CoA dehydrogenase result from charge-transfer interactions in which the ground state is essentially nonbonding as opposed to interactions in which complete electron transfer occurs to form FAD semiquinone. The only significant change in the RR spectrum of FAD on binding to enzyme occurs in the 1250-cm-1 region of the spectrum, a region associated with delta N--H of N-3. The position of this band in fatty acyl-CoA dehydrogenase and the other flavoproteins studied to date is discussed in terms of hydrogen bonding between flavin and protein.", "contents": "Resonance Raman study of flavins and the flavoprotein fatty acyl coenzyme A dehydrogenase. The resonance Raman (RR) spectra of FMN, FAD, FAD in D2O, and 7,8-dimethyl-1, 10-ethyleneisoalloxazinium perchlorate have been obtained by employing KI as a collisional fluorescence-quenching agent. The spectra are very similar to those obtained recently by using the CARS technique to eliminate fluorescence. Spectra have also been obtained for several species in which flavin is known to fluoresce only weakly. We report RR spectra of protonated FMN, FMN semiquinone cation, the general fatty acyl-CoA dehydrogenase, and two \"charge-transfer\" complexes of fatty acyl-CoA dehydrogenase. Tentative assignment of several vibrational bands can be made on the basis of our flavin spectra. RR spectra of fatty acyl-CoA and its complexes are consistent with the previous hypothesis that visible spectral shifts observed during formation of acetoacetyl-CoA and crotonyl-CoA complexes of fatty acyl-CoA dehydrogenase result from charge-transfer interactions in which the ground state is essentially nonbonding as opposed to interactions in which complete electron transfer occurs to form FAD semiquinone. The only significant change in the RR spectrum of FAD on binding to enzyme occurs in the 1250-cm-1 region of the spectrum, a region associated with delta N--H of N-3. The position of this band in fatty acyl-CoA dehydrogenase and the other flavoproteins studied to date is discussed in terms of hydrogen bonding between flavin and protein."}
{"id": "PMID:476065", "title": "Structural and kinetic studies on the solubilization of lecithin by sodium deoxycholate.", "content": "Mixed dispersions of egg phosphatidylcholine (PC) and the bile salt sodium deoxycholate (DOC) were prepared by various methods, and their turbidities and proton magnetic resonance spectra were studied as a function of time. The spectra of dispersions prepared by dissolving both components in a common organic solvent and replacing the organic solvent by water did not change with time, indicating that the mixed aggregates formed represent \"a state of equilibrium\". In the 1H NMR spectra of these mixed aggregates, only signals from small mixed micellar structures were narrow enough to be observed. The dependence of the NMR line widths on the molar ratio of DOC to PC (R) is interpreted in terms of a model for the PC--DOC mixed micelles, according to which PC is arranged as a curved bilayer, the curvature of which increases with increasing R. Upon mixing PC with aqueous solutions of DOC, we found that the mixed aggregates formed are slowly reorganized and ultimately reach the same state of equilibrium. This reorganization was found to be a pseudo-first-order process, the rate constant of which depends linearly upon the detergent concentration. This process involves saturation of the outer bilayers of the multilamellar PC by detergent, followed by transformation of these bilayers into mixed micelles. It is concluded that the solubilization occurs through consecutive \"peeling off\" of lecithin bilayers.", "contents": "Structural and kinetic studies on the solubilization of lecithin by sodium deoxycholate. Mixed dispersions of egg phosphatidylcholine (PC) and the bile salt sodium deoxycholate (DOC) were prepared by various methods, and their turbidities and proton magnetic resonance spectra were studied as a function of time. The spectra of dispersions prepared by dissolving both components in a common organic solvent and replacing the organic solvent by water did not change with time, indicating that the mixed aggregates formed represent \"a state of equilibrium\". In the 1H NMR spectra of these mixed aggregates, only signals from small mixed micellar structures were narrow enough to be observed. The dependence of the NMR line widths on the molar ratio of DOC to PC (R) is interpreted in terms of a model for the PC--DOC mixed micelles, according to which PC is arranged as a curved bilayer, the curvature of which increases with increasing R. Upon mixing PC with aqueous solutions of DOC, we found that the mixed aggregates formed are slowly reorganized and ultimately reach the same state of equilibrium. This reorganization was found to be a pseudo-first-order process, the rate constant of which depends linearly upon the detergent concentration. This process involves saturation of the outer bilayers of the multilamellar PC by detergent, followed by transformation of these bilayers into mixed micelles. It is concluded that the solubilization occurs through consecutive \"peeling off\" of lecithin bilayers."}
{"id": "PMID:476066", "title": "Partial purification and characterization of mammary stimulating factor, a protein which promotes proliferation of mammary epithelium.", "content": "This report describes the partial purification and characterization of mammary stimulating factor (MSF), a mitogenic peptide isolated from serum which initiates growth in mouse mammary epithelium. By using ion-exchange chromatography, gel filtration, and isoelectric focusing, MSF was purified 250-fold from porcine serum. It is a heat-stable protein of molecular weight 10,100--10,400 with an isoelectric point of 5.5--6.0. MSF initiates DNA synthesis in vitro in mammary epithelium to a greater extent than in mouse mammary tumor cells (CZF), 3T3 cells, or chick embryo cells. Comparison of the biological, physical, and immunological properties of MSF with other established growth-promoting peptides suggests that MSF is a unique serum factor.", "contents": "Partial purification and characterization of mammary stimulating factor, a protein which promotes proliferation of mammary epithelium. This report describes the partial purification and characterization of mammary stimulating factor (MSF), a mitogenic peptide isolated from serum which initiates growth in mouse mammary epithelium. By using ion-exchange chromatography, gel filtration, and isoelectric focusing, MSF was purified 250-fold from porcine serum. It is a heat-stable protein of molecular weight 10,100--10,400 with an isoelectric point of 5.5--6.0. MSF initiates DNA synthesis in vitro in mammary epithelium to a greater extent than in mouse mammary tumor cells (CZF), 3T3 cells, or chick embryo cells. Comparison of the biological, physical, and immunological properties of MSF with other established growth-promoting peptides suggests that MSF is a unique serum factor."}
{"id": "PMID:476067", "title": "Evidence of a precursor form of stratum corneum basic protein in rat epidermis.", "content": "The fully differentiated anucleate cells of the stratum corneum of newborn rat epidermis contain a cationic protein called stratum corneum basic protein (SCBP). This protein has a molecular weight (49 000) and an amino acid composition similar to a protein extracted from the less differentiated cell layers of the epidermis. Pulse--chase experiments with radiolabeled histidine were undertaken to test the possiblity that SCBP is derived from a preexisting protein. A protein of 52 000 daltons is rapidly but transiently labeled in extracts of the less differentiated cell layers. As the amount of label in the 52 000-dalton protein decreases, an increase in radiolabel is observed in extracts of the fully differentiated cells. This label is found in SCBP, a protein of lower molecular weight (49 000) than that initially labeled. These proteins are immunologically related and both are resistant to cyanogen bromide cleavage. They differ in apparent molecular weight on sodium dodecyl sulfate--polyacrylamide gels and in their net charge. The results are consistent with the conversion of a precursor protein into SCBP.", "contents": "Evidence of a precursor form of stratum corneum basic protein in rat epidermis. The fully differentiated anucleate cells of the stratum corneum of newborn rat epidermis contain a cationic protein called stratum corneum basic protein (SCBP). This protein has a molecular weight (49 000) and an amino acid composition similar to a protein extracted from the less differentiated cell layers of the epidermis. Pulse--chase experiments with radiolabeled histidine were undertaken to test the possiblity that SCBP is derived from a preexisting protein. A protein of 52 000 daltons is rapidly but transiently labeled in extracts of the less differentiated cell layers. As the amount of label in the 52 000-dalton protein decreases, an increase in radiolabel is observed in extracts of the fully differentiated cells. This label is found in SCBP, a protein of lower molecular weight (49 000) than that initially labeled. These proteins are immunologically related and both are resistant to cyanogen bromide cleavage. They differ in apparent molecular weight on sodium dodecyl sulfate--polyacrylamide gels and in their net charge. The results are consistent with the conversion of a precursor protein into SCBP."}
{"id": "PMID:476068", "title": "Thrombocytin, a serine protease from Bothrops atrox venom. 1. Purification and characterization of the enzyme.", "content": "Thrombocytin, a platelet-activating enzyme from Bothrops atrox venom, has been purified to homogeneity by precipitation with sodium salicylate and chromatography on heparin--agarose. Thrombocytin is a single-chain glycoprotein with a molecular weight of 36 000 which contains 5.6% carbohydrate. It causes platelet aggregation, release of platelet serotonin, and activation of factor XIII. The most sensitive substrate for the amidolytic activity of thrombocytin was Tos-Gly-Pro-Arg-p-nitroanilide hydrochloride. The activity of thrombocytin on this substrate and on platelets was inhibited by diisopropyl fluorophosphate (DFP), soybean trypsin inhibitor, and several arginine chloromethyl ketones. Active site titration with nitrophenyl guanidinobenzoate demonstrated that approximately 86% of the preparation was in the active form. These experiments demonstrate the presence of serine and histidine in the active site of thrombocytin and suggest that thrombocytin is a classical serine protease with a platelet-activating activity similar to thrombin.", "contents": "Thrombocytin, a serine protease from Bothrops atrox venom. 1. Purification and characterization of the enzyme. Thrombocytin, a platelet-activating enzyme from Bothrops atrox venom, has been purified to homogeneity by precipitation with sodium salicylate and chromatography on heparin--agarose. Thrombocytin is a single-chain glycoprotein with a molecular weight of 36 000 which contains 5.6% carbohydrate. It causes platelet aggregation, release of platelet serotonin, and activation of factor XIII. The most sensitive substrate for the amidolytic activity of thrombocytin was Tos-Gly-Pro-Arg-p-nitroanilide hydrochloride. The activity of thrombocytin on this substrate and on platelets was inhibited by diisopropyl fluorophosphate (DFP), soybean trypsin inhibitor, and several arginine chloromethyl ketones. Active site titration with nitrophenyl guanidinobenzoate demonstrated that approximately 86% of the preparation was in the active form. These experiments demonstrate the presence of serine and histidine in the active site of thrombocytin and suggest that thrombocytin is a classical serine protease with a platelet-activating activity similar to thrombin."}
{"id": "PMID:476069", "title": "Thrombocytin, a serine protease from Bothrops atrox venom. 2. Interaction with platelets and plasma-clotting factors.", "content": "Thrombocytin, a serine protease from Bothrops atrox venom, caused platelet aggregation and release of platelet constituents at a concentration of 10(-7) M and clot retraction at a concentration of 2 x 10(-9) M. Thrombocytin was slightly more active when tested on platelets in plasma than on washed platelets suspended in Tyrode--albumin solution. Thrombin was 5 times more active than thrombocytin when tested on platelets in plasma and 50 times more active when tested on washed platelets. The patterns or release induced by thrombocytin and thrombin were similar. Prostaglandin E1 (10(-5) M) produced complete inhibition of platelet release induced by thrombocytin and thrombin. Indomethacin (10(-4) M) was without any effect. Antithrombin III, in the presence of heparin, inhibited the action of thrombocytin on platelets and on a synthetic peptide substrate (Tos-Gly-Pro-Arg-pNA.HCl). formation of an antithrombin III--thrombocytin complex was demonstrated on NaDodSO4--polyacrylamide gel electrophoresis. Hirudin and alpha 1-antitrypsin did not inactivate thrombocytin. Thrombocytin had a low fibrinogen-clotting activity (less than 0.06% that of thrombin). Thrombocytin also caused progressive degradation of the alpha chain of human fibrinogen, and it cleaved prothrombin, releasing products similar to intermediate 1 and fragment 1 produced by thrombin. Thrombocytin activated factor XIII by limited proteolysis and increased the procoagulant activity of factor VIII in a manner analogous to that of thrombin.", "contents": "Thrombocytin, a serine protease from Bothrops atrox venom. 2. Interaction with platelets and plasma-clotting factors. Thrombocytin, a serine protease from Bothrops atrox venom, caused platelet aggregation and release of platelet constituents at a concentration of 10(-7) M and clot retraction at a concentration of 2 x 10(-9) M. Thrombocytin was slightly more active when tested on platelets in plasma than on washed platelets suspended in Tyrode--albumin solution. Thrombin was 5 times more active than thrombocytin when tested on platelets in plasma and 50 times more active when tested on washed platelets. The patterns or release induced by thrombocytin and thrombin were similar. Prostaglandin E1 (10(-5) M) produced complete inhibition of platelet release induced by thrombocytin and thrombin. Indomethacin (10(-4) M) was without any effect. Antithrombin III, in the presence of heparin, inhibited the action of thrombocytin on platelets and on a synthetic peptide substrate (Tos-Gly-Pro-Arg-pNA.HCl). formation of an antithrombin III--thrombocytin complex was demonstrated on NaDodSO4--polyacrylamide gel electrophoresis. Hirudin and alpha 1-antitrypsin did not inactivate thrombocytin. Thrombocytin had a low fibrinogen-clotting activity (less than 0.06% that of thrombin). Thrombocytin also caused progressive degradation of the alpha chain of human fibrinogen, and it cleaved prothrombin, releasing products similar to intermediate 1 and fragment 1 produced by thrombin. Thrombocytin activated factor XIII by limited proteolysis and increased the procoagulant activity of factor VIII in a manner analogous to that of thrombin."}
{"id": "PMID:476072", "title": "Time-resolved resonance Raman characterization of the bO640 intermediate of bacteriorhodopsin. Reprotonation of the Schiff base.", "content": "The resonance Raman spectrum of photolyzed bacteriorhodopsin under conditions known to increase the concentration of the bO640 intermediate in both H2O and D2O is presented. By use of computer subtraction techniques and a knowledge of the Raman spectra of the unphotolyzed bacteriorhodopsin as well as the other intermediates in the cycle, a qualitative spectrum of bO640 is determined. The shift of a band at 1630 cm-1 in H2O to 1616 cm-1 in D2O suggests that the Schiff base of bO640 is protonated. Additional bands at 947, 965, and 992 cm-1 that appear only in D2O suspensions confirm that a proton is coupled to the retinal chromophore of bO640. The reprotonation of the Schiff base thus occurs during the bM412 to bO640 step. The fingerprint region, sensitive to the isomeric configuration of the retinal chromophore of bO640, is dissimilar to the fingerprint regions of published model compounds and other forms of bacteriorhodopsin.", "contents": "Time-resolved resonance Raman characterization of the bO640 intermediate of bacteriorhodopsin. Reprotonation of the Schiff base. The resonance Raman spectrum of photolyzed bacteriorhodopsin under conditions known to increase the concentration of the bO640 intermediate in both H2O and D2O is presented. By use of computer subtraction techniques and a knowledge of the Raman spectra of the unphotolyzed bacteriorhodopsin as well as the other intermediates in the cycle, a qualitative spectrum of bO640 is determined. The shift of a band at 1630 cm-1 in H2O to 1616 cm-1 in D2O suggests that the Schiff base of bO640 is protonated. Additional bands at 947, 965, and 992 cm-1 that appear only in D2O suspensions confirm that a proton is coupled to the retinal chromophore of bO640. The reprotonation of the Schiff base thus occurs during the bM412 to bO640 step. The fingerprint region, sensitive to the isomeric configuration of the retinal chromophore of bO640, is dissimilar to the fingerprint regions of published model compounds and other forms of bacteriorhodopsin."}
{"id": "PMID:476073", "title": "Spectroscopic studies of pyridoxamine (pyridoxine) 5'-phosphate oxidase. Equilibrium dissociation constants and spectra for riboflavin 5'-phosphate and analogues.", "content": "Pyridoxamine (pyridoxine) 5'-phosphate oxidase (EC 1.4.3.5) has been shown to bind 1 mol of riboflavin 5'-phosphate (FMN) per mol of apoenzyme and is active with or inhibited by numerous FMN analogues [Kazarinoff, M. N., & McCormick, D. B. (1975) J. Biol. Chem. 250, 3436--3442]. The KD values and spectra for selected apoenzyme--flavin complexes have been determined and used to elucidate some of the properties of the FMN-binding site of this flavoprotein. Alterations of the pyrimidinoid portion of the flavin ring decrease binding considerably. The absorption spectra for the protein complexes with 3-deaza-FMN and 8-hydroxy-FMN indicate the presence of a dipolar or positively charged protein group near N1 and O2. The substitution of methyl for hydrogen at N3 apparently causes distortion of the interaction between the flavin ring and an active-site aromatic amino acid residue. Although binding is also decreased somewhat by substitutions at postions 8 and 8 alpha, considerable bulk [e.g., 8-(diethylamino)-FMN and 8 alpha-S-(N-acetyl-cysteinyl)-FMN] is accommodated. Hence, this portion of the flavin ring is probably oriented toward, possibly in contact with, solvent, as has been found for the flavodoxins. The importance of optimum interactions between the flavin and the apoprotein is further emphasized by large differences in the activity of flavin analogues that have similar midpoint potentials in solution.", "contents": "Spectroscopic studies of pyridoxamine (pyridoxine) 5'-phosphate oxidase. Equilibrium dissociation constants and spectra for riboflavin 5'-phosphate and analogues. Pyridoxamine (pyridoxine) 5'-phosphate oxidase (EC 1.4.3.5) has been shown to bind 1 mol of riboflavin 5'-phosphate (FMN) per mol of apoenzyme and is active with or inhibited by numerous FMN analogues [Kazarinoff, M. N., & McCormick, D. B. (1975) J. Biol. Chem. 250, 3436--3442]. The KD values and spectra for selected apoenzyme--flavin complexes have been determined and used to elucidate some of the properties of the FMN-binding site of this flavoprotein. Alterations of the pyrimidinoid portion of the flavin ring decrease binding considerably. The absorption spectra for the protein complexes with 3-deaza-FMN and 8-hydroxy-FMN indicate the presence of a dipolar or positively charged protein group near N1 and O2. The substitution of methyl for hydrogen at N3 apparently causes distortion of the interaction between the flavin ring and an active-site aromatic amino acid residue. Although binding is also decreased somewhat by substitutions at postions 8 and 8 alpha, considerable bulk [e.g., 8-(diethylamino)-FMN and 8 alpha-S-(N-acetyl-cysteinyl)-FMN] is accommodated. Hence, this portion of the flavin ring is probably oriented toward, possibly in contact with, solvent, as has been found for the flavodoxins. The importance of optimum interactions between the flavin and the apoprotein is further emphasized by large differences in the activity of flavin analogues that have similar midpoint potentials in solution."}
{"id": "PMID:476074", "title": "Adenosine triphosphate compartmentation in living hearts: a phosphorus nuclear magnetic resonance saturation transfer study.", "content": "31P nuclear magnetic resonance (NMR) studies of creatine phosphokinase (CPK) kinetics using saturation transfer techniques are reported. The phosphocreatine (PCr) and adenosine triphosphate (ATP) levels in perfused hearts can be altered experimentally by stopping the flow of perusate (ischemia) to the heart for 35-min periods, followed by reperfusion to produce stable levels of performance. Utilization of energy by the heart was altered by administration of 25 mM potassium chloride (KCl) in the perfusate, which arrests contraction of the myocardium. Compared with control heart studies, the unidirectional rates measured during ischemia and KCl arrest are altered. The rates observed in the control experiments indicate that the CPK system is not in a steady state. This apparent deviation from steady-state conditions is ascribed to the existence of intracellular compartmentation of ATP.", "contents": "Adenosine triphosphate compartmentation in living hearts: a phosphorus nuclear magnetic resonance saturation transfer study. 31P nuclear magnetic resonance (NMR) studies of creatine phosphokinase (CPK) kinetics using saturation transfer techniques are reported. The phosphocreatine (PCr) and adenosine triphosphate (ATP) levels in perfused hearts can be altered experimentally by stopping the flow of perusate (ischemia) to the heart for 35-min periods, followed by reperfusion to produce stable levels of performance. Utilization of energy by the heart was altered by administration of 25 mM potassium chloride (KCl) in the perfusate, which arrests contraction of the myocardium. Compared with control heart studies, the unidirectional rates measured during ischemia and KCl arrest are altered. The rates observed in the control experiments indicate that the CPK system is not in a steady state. This apparent deviation from steady-state conditions is ascribed to the existence of intracellular compartmentation of ATP."}
{"id": "PMID:476076", "title": "Enthalpy of nucleotides binding to myosin.", "content": "The enthalpies of binding adenosine 5'-diphosphate (ADP) and 5'-adenylyl imidodiphosphate [AMP-P(NH)P] to rabbit skeletal myosin have been measured in Pipes and Tris buffers at pH 7.8 and 15 degrees C. For ADP the enthalpy of binding was exothermic, whereas the enthalpy of binding AMP-P(NH)P, a nonhydrolyzable ATP analogue, was small and endothermic. For the reaction of ATP and myosin, the development of enthalpy was resolved into two phases: a fast endothermic phase, which is the summation of binding and hydrolysis, and a slow exothermic phase, which is associated with product-release steps. These results are discussed in terms of their implications for energy transduction.", "contents": "Enthalpy of nucleotides binding to myosin. The enthalpies of binding adenosine 5'-diphosphate (ADP) and 5'-adenylyl imidodiphosphate [AMP-P(NH)P] to rabbit skeletal myosin have been measured in Pipes and Tris buffers at pH 7.8 and 15 degrees C. For ADP the enthalpy of binding was exothermic, whereas the enthalpy of binding AMP-P(NH)P, a nonhydrolyzable ATP analogue, was small and endothermic. For the reaction of ATP and myosin, the development of enthalpy was resolved into two phases: a fast endothermic phase, which is the summation of binding and hydrolysis, and a slow exothermic phase, which is associated with product-release steps. These results are discussed in terms of their implications for energy transduction."}
{"id": "PMID:476075", "title": "Pyruvate carboxylase from a thermophilic Bacillus: some molecular characteristics.", "content": "Analysis of the native enzyme and of the subunits produced upon its denaturation shows that pyruvate carboxylase from a thermophilic Bacillus is a tetramer with a molecular weight (mean value) of 558,000 and that the four polypeptide subunits are probably identical. The three functions (carboxyl carrier, carboxylation, and carboxyl transfer) in the pyruvate carboxylation reaction must therefore reside in this quarter-molecular polypeptide. The enzyme molecule contains four atoms of zinc and four molecules of D-biotin, and in the electron microscope the disposition of its four subunits presents a rhombic appearance. Reaction of the denatured enzyme with 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) reveals 10 sulfhydryl groups/subunit. In the native enzyme less than one of these groups reacts with DTNB. By contrast, all of these groups (11/subunit) of the native chicken liver pyruvate carboxylase are accessible to DTNB. The thermophile enzyme is also more resistant to other sulfhydryl reagents and to denaturation under certain conditions than the avian enzyme.", "contents": "Pyruvate carboxylase from a thermophilic Bacillus: some molecular characteristics. Analysis of the native enzyme and of the subunits produced upon its denaturation shows that pyruvate carboxylase from a thermophilic Bacillus is a tetramer with a molecular weight (mean value) of 558,000 and that the four polypeptide subunits are probably identical. The three functions (carboxyl carrier, carboxylation, and carboxyl transfer) in the pyruvate carboxylation reaction must therefore reside in this quarter-molecular polypeptide. The enzyme molecule contains four atoms of zinc and four molecules of D-biotin, and in the electron microscope the disposition of its four subunits presents a rhombic appearance. Reaction of the denatured enzyme with 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) reveals 10 sulfhydryl groups/subunit. In the native enzyme less than one of these groups reacts with DTNB. By contrast, all of these groups (11/subunit) of the native chicken liver pyruvate carboxylase are accessible to DTNB. The thermophile enzyme is also more resistant to other sulfhydryl reagents and to denaturation under certain conditions than the avian enzyme."}
{"id": "PMID:476079", "title": "Insulin binding to isolated liver nuclei from obese and lean mice.", "content": "Nuclei isolated from the livers of mice are capable of binding [125I]insulin. A class of high-affinity binding sites having a Kd of 1--2 nM and a capacity of approximately 2000 insulin molecules/nucleus are present on these nuclei. Removal of nuclear membranes by Triton X-100 treatment of the nuclei reduces or eliminates the high-affinity binding sites. Nuclei prepared from livers of the genetically obese mouse (ob/ob) lack, or have markedly reduced numbers of, the high-affinity binding sites whether or not the obese nuclei have been exposed to Triton X-100. The reduced insulin-binding capacity of the obese nuclei correlates with the reported decreased binding of insulin to plasma membranes prepared from target tissue of these animals. The possible physiological significance of nuclear insulin binding is discussed.", "contents": "Insulin binding to isolated liver nuclei from obese and lean mice. Nuclei isolated from the livers of mice are capable of binding [125I]insulin. A class of high-affinity binding sites having a Kd of 1--2 nM and a capacity of approximately 2000 insulin molecules/nucleus are present on these nuclei. Removal of nuclear membranes by Triton X-100 treatment of the nuclei reduces or eliminates the high-affinity binding sites. Nuclei prepared from livers of the genetically obese mouse (ob/ob) lack, or have markedly reduced numbers of, the high-affinity binding sites whether or not the obese nuclei have been exposed to Triton X-100. The reduced insulin-binding capacity of the obese nuclei correlates with the reported decreased binding of insulin to plasma membranes prepared from target tissue of these animals. The possible physiological significance of nuclear insulin binding is discussed."}
{"id": "PMID:476078", "title": "Biosynthesis of slaframine, (1S,6S,8aS)-1-acetoxy-6-aminooctahydroindolizine, a parasympathomimetic alkaloid of fungal origin. 4. Metabolic fate of ethyl pipecolylacetate, 1,3-dioxooctahydroindolizine, and 1-hydroxyoctahydroindolizine in Rhizoctonia leguminicola.", "content": "Known or suspected intermediates in the biosynthesis of slaframine and 3,4,5-trihydroxyoctahydro-1-pyrindine, piperidine alkaloids of the phytopathogenic fungus Rhizoctonia leguminicola, were prepared and tested for biological conversions. Ethyl pipecolylacetate, an analogue of the postulated condensation product of pipecolic and malonic acids (two previously identified alkaloid precursors), was insufficiently stable for feeding experiments. The lactam of pipecolylacetate, 1,3-dioxooctahydroindolizine, was degraded by the fungus without direct incorporation into alkaloids. The known slaframine precursor 1-hydroxyoctahydroindolizine was prepared by a novel route which permitted high levels of deuterium enrichment at C-1 and C-3. Mass spectrometric examination of the slaframine biosynthesized from cis- and trans-[1,3,3-2H]-1-hydroxyoctahydroindolizine strengthened arguments that 1-oxooctahydroindolizine is an intermediate in slaframine biogenesis.", "contents": "Biosynthesis of slaframine, (1S,6S,8aS)-1-acetoxy-6-aminooctahydroindolizine, a parasympathomimetic alkaloid of fungal origin. 4. Metabolic fate of ethyl pipecolylacetate, 1,3-dioxooctahydroindolizine, and 1-hydroxyoctahydroindolizine in Rhizoctonia leguminicola. Known or suspected intermediates in the biosynthesis of slaframine and 3,4,5-trihydroxyoctahydro-1-pyrindine, piperidine alkaloids of the phytopathogenic fungus Rhizoctonia leguminicola, were prepared and tested for biological conversions. Ethyl pipecolylacetate, an analogue of the postulated condensation product of pipecolic and malonic acids (two previously identified alkaloid precursors), was insufficiently stable for feeding experiments. The lactam of pipecolylacetate, 1,3-dioxooctahydroindolizine, was degraded by the fungus without direct incorporation into alkaloids. The known slaframine precursor 1-hydroxyoctahydroindolizine was prepared by a novel route which permitted high levels of deuterium enrichment at C-1 and C-3. Mass spectrometric examination of the slaframine biosynthesized from cis- and trans-[1,3,3-2H]-1-hydroxyoctahydroindolizine strengthened arguments that 1-oxooctahydroindolizine is an intermediate in slaframine biogenesis."}
{"id": "PMID:476077", "title": "Biosynthesis of slaframine, (1S,6S,8aS)-1-acetoxy-6-aminooctahydroindolizine, a parasympathomimetic alkaloid of fungal origin. 3. Origin of the pyrrolidine ring.", "content": "The phytopathogen Rhizoctonia leguminicola has previously been shown to incorporate pipecolic acid into the piperidine alkaloids 1-acetoxy-6-aminooctahydroindolizine (slaframine) and 3,4,5-trihydroxyoctahydro-1-pyrindine. In the experiments described here, resting cultures of R. leguminicola were incubated with [1-14C]- and [2-14C]malonic acid and with [1-14C]- and [2-2H]acetic acid. Both acids were incorporated into the ring systems of both alkaloids. Mass spectrometric analysis of 2H-enriched slaframine showed that the label resides in the five-membered ring and that the methyl carbon of acetate is joined to the carboxyl carbon of pipecolate. A pipecolate-dependent decarboxylation of [1-14C]malonate was demonstrated in cell-free extracts of R. leguminicola. The results account for previously unattributed carbons in the two alkaloids and suggest the formation of an eight-carbon intermediate common to both alkaloids by acylation of malonate with pipecolic acid.", "contents": "Biosynthesis of slaframine, (1S,6S,8aS)-1-acetoxy-6-aminooctahydroindolizine, a parasympathomimetic alkaloid of fungal origin. 3. Origin of the pyrrolidine ring. The phytopathogen Rhizoctonia leguminicola has previously been shown to incorporate pipecolic acid into the piperidine alkaloids 1-acetoxy-6-aminooctahydroindolizine (slaframine) and 3,4,5-trihydroxyoctahydro-1-pyrindine. In the experiments described here, resting cultures of R. leguminicola were incubated with [1-14C]- and [2-14C]malonic acid and with [1-14C]- and [2-2H]acetic acid. Both acids were incorporated into the ring systems of both alkaloids. Mass spectrometric analysis of 2H-enriched slaframine showed that the label resides in the five-membered ring and that the methyl carbon of acetate is joined to the carboxyl carbon of pipecolate. A pipecolate-dependent decarboxylation of [1-14C]malonate was demonstrated in cell-free extracts of R. leguminicola. The results account for previously unattributed carbons in the two alkaloids and suggest the formation of an eight-carbon intermediate common to both alkaloids by acylation of malonate with pipecolic acid."}
{"id": "PMID:476081", "title": "Nucleosome organization during germ cell development in the sea cucumber Holothuria tubulosa.", "content": "Conformational changes that occur in chromatin from developing germ cells of the echinoderm Holothuria tubulosa have been probed with micrococcal nuclease. The results indicate that the extent of DNA degradation to acid-soluble nucleotides is highest in chromatin at the early stages of gonad growth, being drastically subdued in the mature sperm cell. Production of nucleosomal particles also varies with development, involving at least 70% of the chromatin at the final stage of maturation, whereas in immature germ cells it remains much lower. In contrast, electrophoretic analysis for DNA size has shown that the average nucleosome repeat length, about 227 base pairs, does not change throughout the maturation process. However, kinetics of the enzyme reaction have revealed that, although brief digestion of chromatin from both immature gonads and sperm yields comparable series of higher oligomers, extensive digest patterns differ widely. Sperm chromatin, highly protected, releases a 275 base pair intermediate fragment, wholly absent in immature gonads. The 145 base pair core released in both chromatins is not further digested in sperm. In comparison to sperm chromatin, that of immature germ cells is much more susceptible to fragmentation, yielding the usual set of smaller subnucleosomal fragments. These data suggest the induction of differential accessibilities of chromatin DNA with maturation, which is not accompanied by displacement of the histone complement. The histone variants present in this species may well be instrumental in the process.", "contents": "Nucleosome organization during germ cell development in the sea cucumber Holothuria tubulosa. Conformational changes that occur in chromatin from developing germ cells of the echinoderm Holothuria tubulosa have been probed with micrococcal nuclease. The results indicate that the extent of DNA degradation to acid-soluble nucleotides is highest in chromatin at the early stages of gonad growth, being drastically subdued in the mature sperm cell. Production of nucleosomal particles also varies with development, involving at least 70% of the chromatin at the final stage of maturation, whereas in immature germ cells it remains much lower. In contrast, electrophoretic analysis for DNA size has shown that the average nucleosome repeat length, about 227 base pairs, does not change throughout the maturation process. However, kinetics of the enzyme reaction have revealed that, although brief digestion of chromatin from both immature gonads and sperm yields comparable series of higher oligomers, extensive digest patterns differ widely. Sperm chromatin, highly protected, releases a 275 base pair intermediate fragment, wholly absent in immature gonads. The 145 base pair core released in both chromatins is not further digested in sperm. In comparison to sperm chromatin, that of immature germ cells is much more susceptible to fragmentation, yielding the usual set of smaller subnucleosomal fragments. These data suggest the induction of differential accessibilities of chromatin DNA with maturation, which is not accompanied by displacement of the histone complement. The histone variants present in this species may well be instrumental in the process."}
{"id": "PMID:476083", "title": "Chromatin fractionation procedure that yields nucleosomes containing near-stoichiometric amounts of high mobility group nonhistone chromosomal proteins.", "content": "Initial results of an approach to the isolation of functionally active chromatin are described. Slight digestion of mouse myeloma nuclei at 0 degrees C with micrococcal nuclease, followed by dialysis against near-physiological saline solution containing 1 mM Mg2+, caused release of up to 17% of the nuclear DNA as soluble nucleoproteins. This soluble (S) fraction was relatively depleted in H1 histones and methylated DNA (5-methylcytosine) but highly enriched in RNA, single-stranded DNA, and nonhistone chromosomal proteins, particularly two species of the high mobility group identified as HMG 1 and HMG 2. The S fraction released most rapidly (6--8% of the total DNA) consisted mainly of mono- and small oligonucleosomes. The mononucleosomes appeared normal in terms of sedimentation behavior, DNA length, and content of histones H2A, H2B, H3, and H4, but lacked H1, and instead were associated with approximately stoichiometric amounts of HMG 1 and HMG 2. Studies using isolated, fluorescence-labeled, total mouse HMG proteins indicated that added HMG 1 and HMG 2 do not bind strongly to S-fraction nucleoproteins but that two smaller HMG species (probably HMG 14 and HMG 17) do bind preferentially to S-fraction mono- and dinucleosomes. These results argue against artifactual redistribution of HMG 1 and HMG 2 during this fractionation but suggest caution in interpreting the distribution of smaller HMG proteins after digestion of chromatin. The potential relationship of this soluble fraction to transcriptionally active chromatin is discussed.", "contents": "Chromatin fractionation procedure that yields nucleosomes containing near-stoichiometric amounts of high mobility group nonhistone chromosomal proteins. Initial results of an approach to the isolation of functionally active chromatin are described. Slight digestion of mouse myeloma nuclei at 0 degrees C with micrococcal nuclease, followed by dialysis against near-physiological saline solution containing 1 mM Mg2+, caused release of up to 17% of the nuclear DNA as soluble nucleoproteins. This soluble (S) fraction was relatively depleted in H1 histones and methylated DNA (5-methylcytosine) but highly enriched in RNA, single-stranded DNA, and nonhistone chromosomal proteins, particularly two species of the high mobility group identified as HMG 1 and HMG 2. The S fraction released most rapidly (6--8% of the total DNA) consisted mainly of mono- and small oligonucleosomes. The mononucleosomes appeared normal in terms of sedimentation behavior, DNA length, and content of histones H2A, H2B, H3, and H4, but lacked H1, and instead were associated with approximately stoichiometric amounts of HMG 1 and HMG 2. Studies using isolated, fluorescence-labeled, total mouse HMG proteins indicated that added HMG 1 and HMG 2 do not bind strongly to S-fraction nucleoproteins but that two smaller HMG species (probably HMG 14 and HMG 17) do bind preferentially to S-fraction mono- and dinucleosomes. These results argue against artifactual redistribution of HMG 1 and HMG 2 during this fractionation but suggest caution in interpreting the distribution of smaller HMG proteins after digestion of chromatin. The potential relationship of this soluble fraction to transcriptionally active chromatin is discussed."}
{"id": "PMID:476084", "title": "Purification and characterization of the crown gall specific enzyme nopaline synthase.", "content": "Nopaline synthase of sunflower (Helianthus annuus L.) crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37 (nopaline utilizers) was purified to homogeneity as judged by analytical disc gel electrophoresis. The native enzyme elutes from a column of Ultrogen AcA 34 as a single peak with an estimated molecular weight of 158,000. The dissociated enzyme migrates on NaDodSO4-polyacrylamide gels as a single band with a molecular weight of 40,000. Thus, the native enzyme appears to be composed of four equal-weight subunits. Nopaline synthesizing activity is found exclusively in crown gall tissues induced by strains of A. tumefaciens that utilize nopaline (e.g., C58 and T37). We found the same tissue specificity for the purified protein that we believe represents nopaline synthase. The results of kinetic studies of the purified enzyme are consistent with a ter-bi rapid-equilibrium random-order mechanism. Nopaline synthase is probably responsible for the in vivo synthesis of both N2-(1,3-dicarboxypropyl)arginine (nopaline) and N2-(1,3-dicarboxypropyl)ornithine (ornaline) in crown gall tissues since substrate specificities and Km values do not change during purification.", "contents": "Purification and characterization of the crown gall specific enzyme nopaline synthase. Nopaline synthase of sunflower (Helianthus annuus L.) crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37 (nopaline utilizers) was purified to homogeneity as judged by analytical disc gel electrophoresis. The native enzyme elutes from a column of Ultrogen AcA 34 as a single peak with an estimated molecular weight of 158,000. The dissociated enzyme migrates on NaDodSO4-polyacrylamide gels as a single band with a molecular weight of 40,000. Thus, the native enzyme appears to be composed of four equal-weight subunits. Nopaline synthesizing activity is found exclusively in crown gall tissues induced by strains of A. tumefaciens that utilize nopaline (e.g., C58 and T37). We found the same tissue specificity for the purified protein that we believe represents nopaline synthase. The results of kinetic studies of the purified enzyme are consistent with a ter-bi rapid-equilibrium random-order mechanism. Nopaline synthase is probably responsible for the in vivo synthesis of both N2-(1,3-dicarboxypropyl)arginine (nopaline) and N2-(1,3-dicarboxypropyl)ornithine (ornaline) in crown gall tissues since substrate specificities and Km values do not change during purification."}
{"id": "PMID:476085", "title": "Purification and properties of strictosidine synthetase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cells.", "content": "Strictosidine synthetase, which catalyzes the condensation of tryptamine with secologanin to form strictosidine (isovincoside), was purified 740-fold to homogeneity from cultured cells of Catharanthus roseus in 10% yield. The specific activity is 5.85 nkat/mg. The molecular weight as estimated by gel filtration is 38,000. The isoelectric point is 4.6. Apparent Km values for tryptamine and secologanin are 0.83 and 0.46 mM, respectively. The enzyme shows a broad pH optimum between 5.0 and 7.5. The product of the enzymic reaction is exclusively strictosidine, while no trace of its epimer vincoside can be detected. Sulfhydryl inhibitors have no effect on the enzyme. End products in the biosynthetic pathway of indole alkaloids such as ajmalicine, vindoline, and catharanthine do not inhibit the activity of strictosidine synthetase.", "contents": "Purification and properties of strictosidine synthetase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cells. Strictosidine synthetase, which catalyzes the condensation of tryptamine with secologanin to form strictosidine (isovincoside), was purified 740-fold to homogeneity from cultured cells of Catharanthus roseus in 10% yield. The specific activity is 5.85 nkat/mg. The molecular weight as estimated by gel filtration is 38,000. The isoelectric point is 4.6. Apparent Km values for tryptamine and secologanin are 0.83 and 0.46 mM, respectively. The enzyme shows a broad pH optimum between 5.0 and 7.5. The product of the enzymic reaction is exclusively strictosidine, while no trace of its epimer vincoside can be detected. Sulfhydryl inhibitors have no effect on the enzyme. End products in the biosynthetic pathway of indole alkaloids such as ajmalicine, vindoline, and catharanthine do not inhibit the activity of strictosidine synthetase."}
{"id": "PMID:476086", "title": "Conformation of the active site of thiolsubtilisin: reaction with specific chloromethyl ketones and arylacryloylimidazoles.", "content": "The conformation of the active site of thiolsubtilisin, prepared from subtilisin by transformation of the active site Ser to Cys, was compared with that of subtilisin by kinetic and spectroscopic methods. Carbobenzyloxy-L-alanylglycyl-L-phenylalanine chloromethyl ketone inhibited thiolsubtilisin approximately 10(2) times faster than subtilisin; alkylation occurred at the sulfhydryl rather than the imidazolyl group of the active site. pH dependence of the inhibition is different from that of the reaction between a simple thiol with haloacetamide. Furthermore, several native chromophoric arylacryloyl-thiolsubtilisins and arylacryloyl-subtilisins showed similar red shifts when compared with their denatured forms. The rate of deacylation of arylacryloyl-thiolsubtilisins was faster than (or of the same order of magnitude as) the deacylation rate of the analogous arylacryloyl-subtilisins in 30% dioxane (v/v), pH 5--10. The deacylation rate--pH profiles of these arylacryloyl-thiolsubtilisins in 30% dioxane all give pK values of 7.7 which is identical with the pK in the deacylation of acyl-subtilisins. These facts strongly suggest that the active-site conformation remains intact on conversion from subtilisin to thiolsubtilisin. The low esterase and peptidase activities of thiolsubtilisin are most likely due to the relatively low basicity of -SH (compared with -OH).", "contents": "Conformation of the active site of thiolsubtilisin: reaction with specific chloromethyl ketones and arylacryloylimidazoles. The conformation of the active site of thiolsubtilisin, prepared from subtilisin by transformation of the active site Ser to Cys, was compared with that of subtilisin by kinetic and spectroscopic methods. Carbobenzyloxy-L-alanylglycyl-L-phenylalanine chloromethyl ketone inhibited thiolsubtilisin approximately 10(2) times faster than subtilisin; alkylation occurred at the sulfhydryl rather than the imidazolyl group of the active site. pH dependence of the inhibition is different from that of the reaction between a simple thiol with haloacetamide. Furthermore, several native chromophoric arylacryloyl-thiolsubtilisins and arylacryloyl-subtilisins showed similar red shifts when compared with their denatured forms. The rate of deacylation of arylacryloyl-thiolsubtilisins was faster than (or of the same order of magnitude as) the deacylation rate of the analogous arylacryloyl-subtilisins in 30% dioxane (v/v), pH 5--10. The deacylation rate--pH profiles of these arylacryloyl-thiolsubtilisins in 30% dioxane all give pK values of 7.7 which is identical with the pK in the deacylation of acyl-subtilisins. These facts strongly suggest that the active-site conformation remains intact on conversion from subtilisin to thiolsubtilisin. The low esterase and peptidase activities of thiolsubtilisin are most likely due to the relatively low basicity of -SH (compared with -OH)."}
{"id": "PMID:476087", "title": "Protected nucleotide sequences in nuclear ribonucleoprotein.", "content": "The rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for dougble-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.", "contents": "Protected nucleotide sequences in nuclear ribonucleoprotein. The rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for dougble-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence."}
{"id": "PMID:476088", "title": "Rare transfer ribonucleic acid essential for phage growth. Nucleotide sequence comparison of normal and mutant T4 isoleucine-accepting transfer ribonucleic acid.", "content": "One of the eight tRNA species coded by bacteriophage T4 is unique in that (1) it is found in a yield lower by three- to fourfold than that of any other tRNA and (2) while dispensable for growth in standard laboratory hosts, it is essential for phage propagation in a natural isolate of Escherichia coli (strain CT439). We report here the nucleotide sequence of this tRNA and of several mutationally altered forms. The molecule is 77 nucleotides in length and has the anticodon N-A-U. Depending on the pairing properties of the \"wobble\" nucleotide N, this sequence could correspond to one or more of the isoleucine-specific codons (formula: see text) or to the methionine-specific codon A-U-G. Since a T4-specific acceptor activity for isoleucine which is stimulated in ribosome binding by A-U-A but not A-U-U has been reported previously, we infer that we have sequenced a tRNA Ile species which preferentially recognizes A-U-A. Mutant HA1 is unable to grow in CT439; it produces no tRNA Ile. The primary mutational alteration is a transition four residues from the 5'terminus which converts a C.G to a U.G base pair. The consequences of this lesion can be partially reversed by second-site mutations nearby in the acceptor stem. Unexpectedly, the tRNA Ile synthesized in these revertants still retains two unusual structural features found in the wild-type molecule: the opposition of two Up residues in the amino acid acceptor stem and the opposition of an Ap and a Gp residue in the anticodon stem. Implications of these structual anomalies for a possibly unique physiological role of this minor tRNA species are discussed.", "contents": "Rare transfer ribonucleic acid essential for phage growth. Nucleotide sequence comparison of normal and mutant T4 isoleucine-accepting transfer ribonucleic acid. One of the eight tRNA species coded by bacteriophage T4 is unique in that (1) it is found in a yield lower by three- to fourfold than that of any other tRNA and (2) while dispensable for growth in standard laboratory hosts, it is essential for phage propagation in a natural isolate of Escherichia coli (strain CT439). We report here the nucleotide sequence of this tRNA and of several mutationally altered forms. The molecule is 77 nucleotides in length and has the anticodon N-A-U. Depending on the pairing properties of the \"wobble\" nucleotide N, this sequence could correspond to one or more of the isoleucine-specific codons (formula: see text) or to the methionine-specific codon A-U-G. Since a T4-specific acceptor activity for isoleucine which is stimulated in ribosome binding by A-U-A but not A-U-U has been reported previously, we infer that we have sequenced a tRNA Ile species which preferentially recognizes A-U-A. Mutant HA1 is unable to grow in CT439; it produces no tRNA Ile. The primary mutational alteration is a transition four residues from the 5'terminus which converts a C.G to a U.G base pair. The consequences of this lesion can be partially reversed by second-site mutations nearby in the acceptor stem. Unexpectedly, the tRNA Ile synthesized in these revertants still retains two unusual structural features found in the wild-type molecule: the opposition of two Up residues in the amino acid acceptor stem and the opposition of an Ap and a Gp residue in the anticodon stem. Implications of these structual anomalies for a possibly unique physiological role of this minor tRNA species are discussed."}
{"id": "PMID:476089", "title": "Antigody-nucleic acid complexes. Inhibition of translation of silkmoth chorion messenger ribonucleic acid with antibodies specific for 7-methylguanosine.", "content": "Antibodies specific for 7-methylguanosine (m7G) were evaluated for their ability to inhibit the translation of chorion mRNA in a wheat germ, cell-free amino acid incorporating system. Results obtained with antibody concentrations of 0.5--1.5 microM revealed dose-dependent inhibition of [3H]-labeled amino acid incorporation into acid-insoluble radioactivity. Inhibition of translation was attributed to the interaction of anti-m7G antibodies with the 5' termini of chorion mRNAs on the basis that (a) anti-m7G antibodies coupled to Sepharose (anti-m7G-Sepharose) immunospecifically retained 5'-terminal cap structures of chorion mRNAs, i.e., m7G (5')ppp(5')Nm, (b) significant inhibition of translation required a 2-h preincubation of anti-m7G antibodies with mRNA, and (c) similar preincubation periods with anti-m7G antibodies in the presence of the competing nucleoside hapten (m7G) obviated the inhibitory effect of the antibody. The nature of the anti-m7G antibody-mRNA complex was examined by digesting chorion mRNA with nuclease P1 before (predigested) and after (postdigested) immunospecific adsorption to anti-m7G-Sepharose adsorbent. Whereas predigested preparations yielded a single cap structure of the type m7G(5')ppp(5')N, the predominating cap in the postdigested sample was m7G(5')ppp(5')NpNpN. These latter data revealed that the nucleotide sequence adjacent to the cap was not significantly masked by the antibody and suggest the utility of anti-m7G antibody as a site-specific probe.", "contents": "Antigody-nucleic acid complexes. Inhibition of translation of silkmoth chorion messenger ribonucleic acid with antibodies specific for 7-methylguanosine. Antibodies specific for 7-methylguanosine (m7G) were evaluated for their ability to inhibit the translation of chorion mRNA in a wheat germ, cell-free amino acid incorporating system. Results obtained with antibody concentrations of 0.5--1.5 microM revealed dose-dependent inhibition of [3H]-labeled amino acid incorporation into acid-insoluble radioactivity. Inhibition of translation was attributed to the interaction of anti-m7G antibodies with the 5' termini of chorion mRNAs on the basis that (a) anti-m7G antibodies coupled to Sepharose (anti-m7G-Sepharose) immunospecifically retained 5'-terminal cap structures of chorion mRNAs, i.e., m7G (5')ppp(5')Nm, (b) significant inhibition of translation required a 2-h preincubation of anti-m7G antibodies with mRNA, and (c) similar preincubation periods with anti-m7G antibodies in the presence of the competing nucleoside hapten (m7G) obviated the inhibitory effect of the antibody. The nature of the anti-m7G antibody-mRNA complex was examined by digesting chorion mRNA with nuclease P1 before (predigested) and after (postdigested) immunospecific adsorption to anti-m7G-Sepharose adsorbent. Whereas predigested preparations yielded a single cap structure of the type m7G(5')ppp(5')N, the predominating cap in the postdigested sample was m7G(5')ppp(5')NpNpN. These latter data revealed that the nucleotide sequence adjacent to the cap was not significantly masked by the antibody and suggest the utility of anti-m7G antibody as a site-specific probe."}
{"id": "PMID:476090", "title": "High-yield cleavage of tryptophanyl peptide bonds by o-iodosobenzoic acid.", "content": "A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yields in the cleavage are 70--100%. Tryptophanyl bonds to alanine, glycine, serine, threonine, glutamine, arginine, and S-(pyridylethyl)cysteine are split in nearly quantitative yield, while those preceding isoleucine or valine are split in approximately 70% yield in the proteins examined in this work. The chemical mechanism for tryptophanyl bond cleavage has not been defined, but it is likely that oxidation of the indole ring occurs during the reaction with o-iodosobenzoic acid. Some problems with the quality of commercial preparations of the reagent are discussed.", "contents": "High-yield cleavage of tryptophanyl peptide bonds by o-iodosobenzoic acid. A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yields in the cleavage are 70--100%. Tryptophanyl bonds to alanine, glycine, serine, threonine, glutamine, arginine, and S-(pyridylethyl)cysteine are split in nearly quantitative yield, while those preceding isoleucine or valine are split in approximately 70% yield in the proteins examined in this work. The chemical mechanism for tryptophanyl bond cleavage has not been defined, but it is likely that oxidation of the indole ring occurs during the reaction with o-iodosobenzoic acid. Some problems with the quality of commercial preparations of the reagent are discussed."}
{"id": "PMID:476091", "title": "Transfer of singlet energy within trypsin.", "content": "Transfers of singlet energy within trypsin were investigated by measuring the fluorescence absorption anisotropy of its tryptophan residues. A ratio of the anisotropy of trypsin to that for N-acetyl-L-tryptophanamide was determined between 306 and 250 nm. The ratio had an average value of 0.7, whether the trypsin anisotropy was measured at 228 of 296 K. However, trypsin dissolved in 5 M guanidine hydrochloride showed little fluorescence depolarization at 228 K (the anisotropy ratio was approximately equal to 0.9). Thus, there is an extensive conformation-dependent energy transfer between tryptophans in trypsin. The ratio of anisotropies of tyrpsin at 304--270 nm was used to estimate energy transfer from tyrosine to tryptophan. Ratios of 1.8 and 1.7 were obtained at 296 K for the native and guanidinium-unfolded enzyme, respectively. The comparable value for N-acetyl-L-tryptophanamide was 1.7. This indicates that there is little transfer from tyrosine to tryptophan in trypsin at 296 K. As confirmation, the excitation wavelength dependencies of the indole fluorescence quantum yield were the same for native and unfolded trypsin. When experiments were performed at 228 K, the 304--270-nm anisotropy ratios were 2.6 for native and 2.1 for unfolded trypsin at pH2. This indicates that the efficiency of energy transfer from tyrosine to tryptophan increases at low temperatures. A photochemical source of error in the quantitation of the efficiency of energy transfer from tyrosine to tryptophan is also described.", "contents": "Transfer of singlet energy within trypsin. Transfers of singlet energy within trypsin were investigated by measuring the fluorescence absorption anisotropy of its tryptophan residues. A ratio of the anisotropy of trypsin to that for N-acetyl-L-tryptophanamide was determined between 306 and 250 nm. The ratio had an average value of 0.7, whether the trypsin anisotropy was measured at 228 of 296 K. However, trypsin dissolved in 5 M guanidine hydrochloride showed little fluorescence depolarization at 228 K (the anisotropy ratio was approximately equal to 0.9). Thus, there is an extensive conformation-dependent energy transfer between tryptophans in trypsin. The ratio of anisotropies of tyrpsin at 304--270 nm was used to estimate energy transfer from tyrosine to tryptophan. Ratios of 1.8 and 1.7 were obtained at 296 K for the native and guanidinium-unfolded enzyme, respectively. The comparable value for N-acetyl-L-tryptophanamide was 1.7. This indicates that there is little transfer from tyrosine to tryptophan in trypsin at 296 K. As confirmation, the excitation wavelength dependencies of the indole fluorescence quantum yield were the same for native and unfolded trypsin. When experiments were performed at 228 K, the 304--270-nm anisotropy ratios were 2.6 for native and 2.1 for unfolded trypsin at pH2. This indicates that the efficiency of energy transfer from tyrosine to tryptophan increases at low temperatures. A photochemical source of error in the quantitation of the efficiency of energy transfer from tyrosine to tryptophan is also described."}
{"id": "PMID:476093", "title": "A mechanism of erythrocyte lysis by lysophosphatidylcholine.", "content": "Lysophosphatidylcholine micelles liberate several cell surface polypeptides from erythrocyte membranes, inducing a sodium-selective permeability defect which leads to colloid osmotic lysis. Evidence is presented to support the hypothesis that at the lowest lytic lysophospholipid concentrations, selective disruption of membrane protein function, rather than gross structural reorganization of the membrane, is the primary lytic mechanism.", "contents": "A mechanism of erythrocyte lysis by lysophosphatidylcholine. Lysophosphatidylcholine micelles liberate several cell surface polypeptides from erythrocyte membranes, inducing a sodium-selective permeability defect which leads to colloid osmotic lysis. Evidence is presented to support the hypothesis that at the lowest lytic lysophospholipid concentrations, selective disruption of membrane protein function, rather than gross structural reorganization of the membrane, is the primary lytic mechanism."}
{"id": "PMID:476094", "title": "The differential effect of thyrotropin on the electrical responses of thyroid cells in monolayer cultures of varying duration.", "content": "The acute effects of thyrotropin on the membrane potential of thyroid cells maintained in the presence or absence of thyrotropin (0.2 U/ml) in the culture medium was determined. Monolayer cultures were prepared from porcine thyroid glands and cultured for 4--17 days after which the culture medium was exchanged for a buffered salt solution for intracellular measurements of the membrane potential. Cells were serially impaled with a microelectrode, first in the absence and then in the presence of 10 mU/ml thyrotropin. Cells cultured for 4--9 days depolarized from --29.6 +/- 1.7 (mean +/- S.E.) to --19.3 +/- 1.3 mV within 10 min after acute addition of 10 mU/ml thyrotropin. From 11 to 17 days of culture, basal membrane potentials were lower and, in most instances, cell hyperpolarization occurred within 30 min in response to thyrotropin. There was no difference in the electrical response of cells maintained in culture with or without thyrotropin. However, cells cultured with thyrotropin formed follicle-like structures in contrast to the monolayer formation of cells cultured without thyrotropin. The changes in the basal and stimulated electrical responses occur within a time frame similar to that reported for changes in the biosynthetic capacity of thyroid cells in culture. The data further emphasize the possible regulatory role of the cell membrane in stimulus-secretion coupling in the thyroid.", "contents": "The differential effect of thyrotropin on the electrical responses of thyroid cells in monolayer cultures of varying duration. The acute effects of thyrotropin on the membrane potential of thyroid cells maintained in the presence or absence of thyrotropin (0.2 U/ml) in the culture medium was determined. Monolayer cultures were prepared from porcine thyroid glands and cultured for 4--17 days after which the culture medium was exchanged for a buffered salt solution for intracellular measurements of the membrane potential. Cells were serially impaled with a microelectrode, first in the absence and then in the presence of 10 mU/ml thyrotropin. Cells cultured for 4--9 days depolarized from --29.6 +/- 1.7 (mean +/- S.E.) to --19.3 +/- 1.3 mV within 10 min after acute addition of 10 mU/ml thyrotropin. From 11 to 17 days of culture, basal membrane potentials were lower and, in most instances, cell hyperpolarization occurred within 30 min in response to thyrotropin. There was no difference in the electrical response of cells maintained in culture with or without thyrotropin. However, cells cultured with thyrotropin formed follicle-like structures in contrast to the monolayer formation of cells cultured without thyrotropin. The changes in the basal and stimulated electrical responses occur within a time frame similar to that reported for changes in the biosynthetic capacity of thyroid cells in culture. The data further emphasize the possible regulatory role of the cell membrane in stimulus-secretion coupling in the thyroid."}
{"id": "PMID:476095", "title": "The modulation of membrane fluidity by hydrogenation processes. III. The hydrogenation of biomembranes of spinach chloroplasts and a study of the effect of this on photosynthetic electron transport.", "content": "A method is reported for the in situ modification of the lipids of isolated spinach chloroplast membranes. The technique is based on a direct hydrogenation of the lipid double bonds in the presence of the catalyst, chlorotris(triphenylphosphine)rhodium (I). The pattern of hydrogenation achieved suggests that the catalyst distributes amongst all of the membranes. The polyunsaturated lipids within the membranes are hydrogenated at a faster rate and at an earlier stage than are the monoenoic lipids. Whilst addition of the catalyst to the chloroplast causes an initial 10--20% decrease in Hill activity, saturation of up to 40% of the double bonds present can be accomplished without causing further significant alterations in photosynthetic electron transport processes or marked morphological changes of the chloroplast structure as observed in the electron microscope.", "contents": "The modulation of membrane fluidity by hydrogenation processes. III. The hydrogenation of biomembranes of spinach chloroplasts and a study of the effect of this on photosynthetic electron transport. A method is reported for the in situ modification of the lipids of isolated spinach chloroplast membranes. The technique is based on a direct hydrogenation of the lipid double bonds in the presence of the catalyst, chlorotris(triphenylphosphine)rhodium (I). The pattern of hydrogenation achieved suggests that the catalyst distributes amongst all of the membranes. The polyunsaturated lipids within the membranes are hydrogenated at a faster rate and at an earlier stage than are the monoenoic lipids. Whilst addition of the catalyst to the chloroplast causes an initial 10--20% decrease in Hill activity, saturation of up to 40% of the double bonds present can be accomplished without causing further significant alterations in photosynthetic electron transport processes or marked morphological changes of the chloroplast structure as observed in the electron microscope."}
{"id": "PMID:476097", "title": "Thermotropic phase transitions of phosphatidylcholines with odd-numbered n-acyl chains.", "content": "Diacyl phosphatidylcholines with n-C13, -C15, -C17 and -C19 saturated acyl chains have been synthesized and their phase transitions in the presence of excess water monitored by differential thermal analysis. The C15-, C17- and C19-diacyl species show gel to liquid-crystalline transitions and pretransitions like those of the even-chain phosphatidylcholines. A plot of the main phase transition temperature, Tc, vs. acyl chain length is a smooth curve on which the Tc values of both odd- and even-chain species fall, while a similar plot for the pretransition temperature, Tp, shows significant alternation of Tp between odd- and even-chain species. Consideration of these results in terms of the physical basis of the odd-even alternation of phase transition temperatures in homologous series of paraffinic compounds suggests that the acyl chains of disaturated phosphatidylcholines are tilted with respect to the bilayer normal below Tp but become perpendicular to the bilayer surface above the pretransition temperature.", "contents": "Thermotropic phase transitions of phosphatidylcholines with odd-numbered n-acyl chains. Diacyl phosphatidylcholines with n-C13, -C15, -C17 and -C19 saturated acyl chains have been synthesized and their phase transitions in the presence of excess water monitored by differential thermal analysis. The C15-, C17- and C19-diacyl species show gel to liquid-crystalline transitions and pretransitions like those of the even-chain phosphatidylcholines. A plot of the main phase transition temperature, Tc, vs. acyl chain length is a smooth curve on which the Tc values of both odd- and even-chain species fall, while a similar plot for the pretransition temperature, Tp, shows significant alternation of Tp between odd- and even-chain species. Consideration of these results in terms of the physical basis of the odd-even alternation of phase transition temperatures in homologous series of paraffinic compounds suggests that the acyl chains of disaturated phosphatidylcholines are tilted with respect to the bilayer normal below Tp but become perpendicular to the bilayer surface above the pretransition temperature."}
{"id": "PMID:476096", "title": "Physicochemical characterization of 1,2-diphytanoyl-sn-glycero-3-phosphocholine in model membrane systems.", "content": "We report here on a series of studies aimed at characterization of the structural and dynamical properties of the synthetic lipid diphytanoyl phosphatidylcholine, in multilamellar dispersions and vesicle suspensions. The lipid exhibits no detectable gel to liquid crystalline phase transition over a large temperature range (-120 degrees C to +120 degrees C). Examination of proton nuclear magnetic resonance (NMR) free induction decays obtained from multilayer dispersions of diphytanoyl phosphatidylcholine provided an estimate of the methylene proton order parameter. The estimated magnitude of 0.21 is comparable to those determined for other phospholipids. Sonication of aqueous dispersions of diphytanoyl phosphatidylcholine led to formation of bilayer vesicles as determined by the measurement of the outer/inner choline methyl proton resonances, vesicle sizes in electron micrographs, and comparison of proton NMR linewidths between multilayer and sonicated dispersions. Ultracentrifugation studies of diphytanoyl phosphatidylcholine vesicles in H2O and 2H2O media yielded a value of 1.013 +/- 0.026 ml/g for the partial specific volume of this lipid. We have measured spin lattice relaxation rates for the methyl and methylenemethyne protons of the hydrocarbon chains of diphytanoyl phosphatidylcholine in bilayer vesicles over a range of temperatures and at two NMR frequencies (100 and 220 MHz). The observed relaxation rates for the methylene protons in this system were approximately twice those previously reported for dipalmitoyl phosphatidylcholine at comparable temperatures and resonance frequencies, whereas the relaxation rates measured for the methyl protons were greater than those of the straight chain lipid by an order of magnitude. Measurement of the spin lattice relaxation rates of the hydrocarbon protons of the diphytanoyl phosphatidylcholine in a 10 mol% mixture of the branched-chain lipid in a deuterated host lipid, diperdeuteropalmitoyl phosphatidylcholine, showed a discontinuity in the temperature dependence of the proton NMR longitudinal relaxation rates of the branched-chain lipid in the region of the gel to liquid crystalline phase transition temperature of the deuterated dipalmitoyl phosphatidylcholine host lipid. This result may be taken as evidence of lateral phase separation of a liquid cyrstalline phase enriched in diphytanoyl phosphatidylcholine from a gel phase enriched in diperdeuteropalmitoyl phosphatidylcholine at temperatures below the phase transition temperature of deuterated host lipid. This conclusion is supported by the observation of an abrupt change in the hydrocarbon methylene linewidth (at 100 MHz) of 10 mol% diphytanoyl phosphatidylcholine in diperdeuteropalmitoyl phosphatidylcholine over the temperature range where lateral phase separation is taking place according to differential thermograms.", "contents": "Physicochemical characterization of 1,2-diphytanoyl-sn-glycero-3-phosphocholine in model membrane systems. We report here on a series of studies aimed at characterization of the structural and dynamical properties of the synthetic lipid diphytanoyl phosphatidylcholine, in multilamellar dispersions and vesicle suspensions. The lipid exhibits no detectable gel to liquid crystalline phase transition over a large temperature range (-120 degrees C to +120 degrees C). Examination of proton nuclear magnetic resonance (NMR) free induction decays obtained from multilayer dispersions of diphytanoyl phosphatidylcholine provided an estimate of the methylene proton order parameter. The estimated magnitude of 0.21 is comparable to those determined for other phospholipids. Sonication of aqueous dispersions of diphytanoyl phosphatidylcholine led to formation of bilayer vesicles as determined by the measurement of the outer/inner choline methyl proton resonances, vesicle sizes in electron micrographs, and comparison of proton NMR linewidths between multilayer and sonicated dispersions. Ultracentrifugation studies of diphytanoyl phosphatidylcholine vesicles in H2O and 2H2O media yielded a value of 1.013 +/- 0.026 ml/g for the partial specific volume of this lipid. We have measured spin lattice relaxation rates for the methyl and methylenemethyne protons of the hydrocarbon chains of diphytanoyl phosphatidylcholine in bilayer vesicles over a range of temperatures and at two NMR frequencies (100 and 220 MHz). The observed relaxation rates for the methylene protons in this system were approximately twice those previously reported for dipalmitoyl phosphatidylcholine at comparable temperatures and resonance frequencies, whereas the relaxation rates measured for the methyl protons were greater than those of the straight chain lipid by an order of magnitude. Measurement of the spin lattice relaxation rates of the hydrocarbon protons of the diphytanoyl phosphatidylcholine in a 10 mol% mixture of the branched-chain lipid in a deuterated host lipid, diperdeuteropalmitoyl phosphatidylcholine, showed a discontinuity in the temperature dependence of the proton NMR longitudinal relaxation rates of the branched-chain lipid in the region of the gel to liquid crystalline phase transition temperature of the deuterated dipalmitoyl phosphatidylcholine host lipid. This result may be taken as evidence of lateral phase separation of a liquid cyrstalline phase enriched in diphytanoyl phosphatidylcholine from a gel phase enriched in diperdeuteropalmitoyl phosphatidylcholine at temperatures below the phase transition temperature of deuterated host lipid. This conclusion is supported by the observation of an abrupt change in the hydrocarbon methylene linewidth (at 100 MHz) of 10 mol% diphytanoyl phosphatidylcholine in diperdeuteropalmitoyl phosphatidylcholine over the temperature range where lateral phase separation is taking place according to differential thermograms."}
{"id": "PMID:476099", "title": "Negatively charged phospholipids and their position in the cholesterol affinity sequence.", "content": "The effects of low concentrations of cholesterol in mixtures of a negatively charged phospholipid (phosphatidylserine or phosphatidylglycerol) and another phospholipid (phosphatidylcholine, sphingomyelin or phosphatidylethanolamine) have been studied by differential scanning calorimetry. Only mixtures which showed a gel phase miscibility gap have been employed. It was demonstrated that in mixtures with phosphatidylethanolamine, cholesterol was preferentially associated with the negatively charged phospholipid, regardless whether this species represented the component with the high or with the low transition temperature in the mixture. In mixtures of a negatively charged phospholipid and phosphatidylcholine, cholesterol associated with the negatively charged phospholipid; when the phosphatidylcholine was the species with the low transition temperature, cholesterol had an affinity for the phosphatidylcholine and for the negatively charged phospholipid as well. Cholesterol, in a mixture of sphingomyeline with a high and phosphatidylserine with a low transition temperature, was preferentially associated with sphingomyelin. From these experiments it is concluded that phospholipids show a decrease in affinity for cholesterol in the following order: sphingomyelin greater than phosphatidylserine, phosphatidylglycerol greater than phosphatidylcholine greater than phosphatidylethanolamine.", "contents": "Negatively charged phospholipids and their position in the cholesterol affinity sequence. The effects of low concentrations of cholesterol in mixtures of a negatively charged phospholipid (phosphatidylserine or phosphatidylglycerol) and another phospholipid (phosphatidylcholine, sphingomyelin or phosphatidylethanolamine) have been studied by differential scanning calorimetry. Only mixtures which showed a gel phase miscibility gap have been employed. It was demonstrated that in mixtures with phosphatidylethanolamine, cholesterol was preferentially associated with the negatively charged phospholipid, regardless whether this species represented the component with the high or with the low transition temperature in the mixture. In mixtures of a negatively charged phospholipid and phosphatidylcholine, cholesterol associated with the negatively charged phospholipid; when the phosphatidylcholine was the species with the low transition temperature, cholesterol had an affinity for the phosphatidylcholine and for the negatively charged phospholipid as well. Cholesterol, in a mixture of sphingomyeline with a high and phosphatidylserine with a low transition temperature, was preferentially associated with sphingomyelin. From these experiments it is concluded that phospholipids show a decrease in affinity for cholesterol in the following order: sphingomyelin greater than phosphatidylserine, phosphatidylglycerol greater than phosphatidylcholine greater than phosphatidylethanolamine."}
{"id": "PMID:476098", "title": "The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles.", "content": "Triton X-100 (in concentrations which did not cause a significant solubilization of membrane material) caused aggregation of the intramembrane particles of human erythrocyte ghosts. Ghosts from which the extrinsic proteins had been removed by alkali treatment showed a temperature-induced aggregation of the particles. With virtually no spectrin present, the particles in these stripped ghosts could still be aggregated by manipulations with ionic strength and pH, or by the addition of calcium. Recombinant vesicles were made from a Triton X-100 extract and a mixture of phospholipids with a composition which resembled that of the inner monolayer of erythrocyte membrane. In these recombinants the same manipulations with ionic strength and pH and the addition of calcium caused a rearrangement of the particles, resulting in the appearance of particle-free areas. In recombinants prepared from a Triton X-100 extract and egg phosphatidylcholine the lateral distribution of the particles was not altered by these manipulations. It is concluded that in the erythrocyte membrane the intramembrane particles can be aggregated by effects of external agents on lipid components. In this light the role of spectrin in stabilizing the membrane by interactions with lipids in the inner monolayer is discussed.", "contents": "The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles. Triton X-100 (in concentrations which did not cause a significant solubilization of membrane material) caused aggregation of the intramembrane particles of human erythrocyte ghosts. Ghosts from which the extrinsic proteins had been removed by alkali treatment showed a temperature-induced aggregation of the particles. With virtually no spectrin present, the particles in these stripped ghosts could still be aggregated by manipulations with ionic strength and pH, or by the addition of calcium. Recombinant vesicles were made from a Triton X-100 extract and a mixture of phospholipids with a composition which resembled that of the inner monolayer of erythrocyte membrane. In these recombinants the same manipulations with ionic strength and pH and the addition of calcium caused a rearrangement of the particles, resulting in the appearance of particle-free areas. In recombinants prepared from a Triton X-100 extract and egg phosphatidylcholine the lateral distribution of the particles was not altered by these manipulations. It is concluded that in the erythrocyte membrane the intramembrane particles can be aggregated by effects of external agents on lipid components. In this light the role of spectrin in stabilizing the membrane by interactions with lipids in the inner monolayer is discussed."}
{"id": "PMID:476100", "title": "Inside-outside distribution and diffusion of phosphatidylcholine in rat sarcoplasmic reticulum as determined by 13C NMR and phosphatidylcholine exchange protein.", "content": "1. The transverse distribution of phosphatidylcholine in rat sarcoplasmic reticulum was investigated employing 13C NMR in conjunction with the shift reagent DyCl3. 2. Sarcoplasmic reticulum phosphatidylcholine was enriched with 13C by feeding rats a diet containing [N-Me3-13C]choline. Up to 32% of the sarcoplasmic reticulum phosphatidyl-[N-Me3-12C]choline was replaced by phosphatidyl-[N-Me3-13C]choline. 3. Titration of 13C-enriched sarcoplasmic reticulum with Dy3+ indicates that 40% of the phosphatidyl-[N-Me3-13C]choline is exposed to the external medium, whereas 60% is shielded from interaction with Dy3+. 4. Incubation of 32P-labelled sarcoplasmic reticulum with excess mitochondria and phosphatidylcholine exchange protein results in a fast transport of approx. 80% of [32P]phosphatydylcholine to the mitochondria indicating that part of the phosphatidylcholine pool is involved in a rapid transbilayer movement.", "contents": "Inside-outside distribution and diffusion of phosphatidylcholine in rat sarcoplasmic reticulum as determined by 13C NMR and phosphatidylcholine exchange protein. 1. The transverse distribution of phosphatidylcholine in rat sarcoplasmic reticulum was investigated employing 13C NMR in conjunction with the shift reagent DyCl3. 2. Sarcoplasmic reticulum phosphatidylcholine was enriched with 13C by feeding rats a diet containing [N-Me3-13C]choline. Up to 32% of the sarcoplasmic reticulum phosphatidyl-[N-Me3-12C]choline was replaced by phosphatidyl-[N-Me3-13C]choline. 3. Titration of 13C-enriched sarcoplasmic reticulum with Dy3+ indicates that 40% of the phosphatidyl-[N-Me3-13C]choline is exposed to the external medium, whereas 60% is shielded from interaction with Dy3+. 4. Incubation of 32P-labelled sarcoplasmic reticulum with excess mitochondria and phosphatidylcholine exchange protein results in a fast transport of approx. 80% of [32P]phosphatydylcholine to the mitochondria indicating that part of the phosphatidylcholine pool is involved in a rapid transbilayer movement."}
{"id": "PMID:476102", "title": "The occurrence of lipidic particles in lipid bilayers as seen by 31P NMR and freeze-fracture electron-microscopy.", "content": "A new type of lipid organization is observed in mixtures of phosphatidyl-choline with cardiolipin (in the presence of Ca2+), monoglycosyldiglyceride and phosphatidylethanolamine (in the presence of cholesterol). This phase is characterised by an isotropic 31P NMR signal and is visualised by freeze-fracturing as particles and pits on the fracture faces of the lipid bilayer. As the most favourable model for this phase we propose the inverted micelle sandwiched in between the two monolayers of the lipid bilayer.", "contents": "The occurrence of lipidic particles in lipid bilayers as seen by 31P NMR and freeze-fracture electron-microscopy. A new type of lipid organization is observed in mixtures of phosphatidyl-choline with cardiolipin (in the presence of Ca2+), monoglycosyldiglyceride and phosphatidylethanolamine (in the presence of cholesterol). This phase is characterised by an isotropic 31P NMR signal and is visualised by freeze-fracturing as particles and pits on the fracture faces of the lipid bilayer. As the most favourable model for this phase we propose the inverted micelle sandwiched in between the two monolayers of the lipid bilayer."}
{"id": "PMID:476101", "title": "Transverse distribution and movement of lysophosphatidylcholine in sarcoplasmic reticulum membranes as determined by 13C NMR and lysophospholipase.", "content": "1. The transverse distribution of 1-palmitoyl-sn-glycero-3-phospho-N-[Me-13C]-choline in vitro incorporated in sarcoplasmic reticulum has been measured by means of 13C NMR and DyCl3 as an impermeable shift reagent. 2. Lysophosphatidylcholine added to the membranes equilibrates within 30 min at 20 degrees C between outer and inner membrane leaflet so that 42% is located in the inner leaflet. 3. Lysophosphatidylcholine diffuses back from the inner leaflet to the outer upon lysophospholipase action on the outer lysophosphatidylcholine pool.", "contents": "Transverse distribution and movement of lysophosphatidylcholine in sarcoplasmic reticulum membranes as determined by 13C NMR and lysophospholipase. 1. The transverse distribution of 1-palmitoyl-sn-glycero-3-phospho-N-[Me-13C]-choline in vitro incorporated in sarcoplasmic reticulum has been measured by means of 13C NMR and DyCl3 as an impermeable shift reagent. 2. Lysophosphatidylcholine added to the membranes equilibrates within 30 min at 20 degrees C between outer and inner membrane leaflet so that 42% is located in the inner leaflet. 3. Lysophosphatidylcholine diffuses back from the inner leaflet to the outer upon lysophospholipase action on the outer lysophosphatidylcholine pool."}
{"id": "PMID:476104", "title": "Calcium transport by pigeon erythrocyte membrane vesicles.", "content": "Membrane vesicles from pigeon erythrocytes show a rapid, ATP-dependent accumulation of 45Ca2+.Ca2+ accumulation ratios greater than or approximately equal to 10(4) are readily attained. For ATP-dependent Ca2+ uptake, V is 1.5 mmol.1(-1).min(-1) at 27 degrees C (approx. 0.9 nmol.mg-1 protein.min-1), [Ca2+]1/2 is 0.18 microM, [ATP]1/2 is 30--60 microM, the Ca2+ uptake rate depends on [Ca2+]2 and the dependence of uptake rate on ATP concentration implies strong ATP-ATP cooperativity. The Arrhenius activation energy is 19.1 +/- 1.4 kcal/mol and the pH optimum is approx. 6.9.", "contents": "Calcium transport by pigeon erythrocyte membrane vesicles. Membrane vesicles from pigeon erythrocytes show a rapid, ATP-dependent accumulation of 45Ca2+.Ca2+ accumulation ratios greater than or approximately equal to 10(4) are readily attained. For ATP-dependent Ca2+ uptake, V is 1.5 mmol.1(-1).min(-1) at 27 degrees C (approx. 0.9 nmol.mg-1 protein.min-1), [Ca2+]1/2 is 0.18 microM, [ATP]1/2 is 30--60 microM, the Ca2+ uptake rate depends on [Ca2+]2 and the dependence of uptake rate on ATP concentration implies strong ATP-ATP cooperativity. The Arrhenius activation energy is 19.1 +/- 1.4 kcal/mol and the pH optimum is approx. 6.9."}
{"id": "PMID:476103", "title": "Molecular and biosynthetic heterogeneity of fucosyl glycoproteins associated with rat brain synaptic functions.", "content": "The composition and biosynthesis of fucosyl glycoproteins present in rat brain synaptic membranes and synaptic junctions were investigated. Reaction with 125I-labelled fucose-binding protein (Lotus tetragonolobus) following sodium dodecyl sulphate gel electrophoresis identified 6--8 fucosyl glycoproteins in synaptic membranes but only three major high molecular classes (Mr = 180 000, 130 000 and 110 000) in synaptic junctions. Affinity chromatography on concanavalin A-Sepharose resolved each of the synaptic junctional fucosyl glycoproteins into concanavalin A-positive and negative components indicating the presence of at least six high molecular weight fucosyl glycoproteins in synaptic junctions. Following the administration of [3H]fucose synaptic membranes, synaptic junctions and post-synaptic densities incorporated isotope, the order of relative specific activities being synaptic membranes greater than synaptic junctions greater than post-synaptic densities. Fractionation of [3H]fucose-labelled synaptic junctions on concanavalin A-Sepharose revealed a time-dependent increase in the percentage of isotope associated with the concanavalin A-positive glycoproteins. The results demonstrate both molecular and biosynthetic heterogeneity of fucosyl glycoproteins associated with synaptic junctions.", "contents": "Molecular and biosynthetic heterogeneity of fucosyl glycoproteins associated with rat brain synaptic functions. The composition and biosynthesis of fucosyl glycoproteins present in rat brain synaptic membranes and synaptic junctions were investigated. Reaction with 125I-labelled fucose-binding protein (Lotus tetragonolobus) following sodium dodecyl sulphate gel electrophoresis identified 6--8 fucosyl glycoproteins in synaptic membranes but only three major high molecular classes (Mr = 180 000, 130 000 and 110 000) in synaptic junctions. Affinity chromatography on concanavalin A-Sepharose resolved each of the synaptic junctional fucosyl glycoproteins into concanavalin A-positive and negative components indicating the presence of at least six high molecular weight fucosyl glycoproteins in synaptic junctions. Following the administration of [3H]fucose synaptic membranes, synaptic junctions and post-synaptic densities incorporated isotope, the order of relative specific activities being synaptic membranes greater than synaptic junctions greater than post-synaptic densities. Fractionation of [3H]fucose-labelled synaptic junctions on concanavalin A-Sepharose revealed a time-dependent increase in the percentage of isotope associated with the concanavalin A-positive glycoproteins. The results demonstrate both molecular and biosynthetic heterogeneity of fucosyl glycoproteins associated with synaptic junctions."}
{"id": "PMID:476105", "title": "Active Ca2+ transport by vesicles reconstituted from Triton X-100-solubilized pigeon erythrocyte membrane.", "content": "Pigeon erythrocyte membrane was solubilized partially, but relatively unselectively by Triton X-100. Vesicles were reconstituted from mixtures of Triton-solubilized membrane and lipid (phosphatidylcholine plus phosphatidyl-ethanolamine plus cholesterol) by addition of bovine high-density lipoprotein. This efficiently removed the Triton X-100. Sodium dodecyl sulfate-polyacrylamide gel electropherograms of reconstituted vesicles showed band patterns resembling those of the original membrane. The reconstituted vesicles showed ATP-dependent active accumulation of 45Ca2+. ATP-dependent 45Ca2+ uptake by the reconstituted vesicles resembled the corresponding activity of the original membrane vesicles; in both preparations the Ca2+ uptake rate depended on the square of the Ca2+ concentration and had similar [Ca2+]1/2 values, 0.16 microM and 0.18 microM, respectively.", "contents": "Active Ca2+ transport by vesicles reconstituted from Triton X-100-solubilized pigeon erythrocyte membrane. Pigeon erythrocyte membrane was solubilized partially, but relatively unselectively by Triton X-100. Vesicles were reconstituted from mixtures of Triton-solubilized membrane and lipid (phosphatidylcholine plus phosphatidyl-ethanolamine plus cholesterol) by addition of bovine high-density lipoprotein. This efficiently removed the Triton X-100. Sodium dodecyl sulfate-polyacrylamide gel electropherograms of reconstituted vesicles showed band patterns resembling those of the original membrane. The reconstituted vesicles showed ATP-dependent active accumulation of 45Ca2+. ATP-dependent 45Ca2+ uptake by the reconstituted vesicles resembled the corresponding activity of the original membrane vesicles; in both preparations the Ca2+ uptake rate depended on the square of the Ca2+ concentration and had similar [Ca2+]1/2 values, 0.16 microM and 0.18 microM, respectively."}
{"id": "PMID:476106", "title": "Effect of cholesterol on the valinomycin-mediated uptake of rubidium into erythrocytes and phospholipid vesicles.", "content": "Human erythrocytes have been treated with lipid vesicles in order to alter the cholesterol content of the cell membrane. Erythrocytes have been produced with cholesterol concentrations between 33 and 66 mol% of total lipid. The rate of valinomycin-mediated uptake of rubidium into the red cells at 37 degrees C was lowered by increasing the cholesterol concentration of the cell membrane. Cholesterol increased the permeability to valinomycin at 20 degrees C of small (less than 50 nm), unilamellar egg phosphatidylcholine vesicles formed by sonication. Cholesterol decreased the permeability to valinomycin at 20 degrees C of large (up to 200 nm) unilamellar egg phosphatidylcholine vesicles formed by freeze-thaw plus brief sonication. It is concluded that cholesterol increases the permeability of small membrane vesicles to hydrophobic penetrating substances while above the transition temperature but has the opposite effect on large membrane vesicles and on the membranes of even larger cells.", "contents": "Effect of cholesterol on the valinomycin-mediated uptake of rubidium into erythrocytes and phospholipid vesicles. Human erythrocytes have been treated with lipid vesicles in order to alter the cholesterol content of the cell membrane. Erythrocytes have been produced with cholesterol concentrations between 33 and 66 mol% of total lipid. The rate of valinomycin-mediated uptake of rubidium into the red cells at 37 degrees C was lowered by increasing the cholesterol concentration of the cell membrane. Cholesterol increased the permeability to valinomycin at 20 degrees C of small (less than 50 nm), unilamellar egg phosphatidylcholine vesicles formed by sonication. Cholesterol decreased the permeability to valinomycin at 20 degrees C of large (up to 200 nm) unilamellar egg phosphatidylcholine vesicles formed by freeze-thaw plus brief sonication. It is concluded that cholesterol increases the permeability of small membrane vesicles to hydrophobic penetrating substances while above the transition temperature but has the opposite effect on large membrane vesicles and on the membranes of even larger cells."}
{"id": "PMID:476108", "title": "Exchange and interactions between lipid layers at the surface of a liposome solution.", "content": "Lipid organization and lipid transport processes occurring at the air-water interface of a liposome (lipid vesicle) solution are studied by conventional surface pressure-area measurements and interpreted by an adequate theory. At the interface of a dioleoyl phosphatidylcholine vesicle solution, used for demonstration, a well defined two layer structure selfassembles: vesicles disintegrate at the interface forming a surface-adsorbed lipid monolayer, which prevents further disintegration beyond about 1 dyne/cm surface pressure. A layer of vesicles now assembles in close association with the monolayer. This layer is in vesicle diffusion exchange with the solution and in lipid exchange with the monolayer. The lipid exchange occurs exclusively between the monolayer and the outer lipid layer of the vesicles; it is absent between outer and inner vesicle layers. Equilibration of the lipid density in the monolayer with that in the vesicle outer layer provides a coherent and quantitative explanation of the observed hysteresis effects and equilibrium states. The correspondence between monolayer and vesicle outer layer is traced down to equilibrium constants and rate constants and their dependences on surface pressure, vesicle size and concentration. Other alternate realizations of surface structure and exchange, including induced lipid flip-flop within vesicles or vesicle monolayer adhesion or fusion are potential applications of the proposed analysis.", "contents": "Exchange and interactions between lipid layers at the surface of a liposome solution. Lipid organization and lipid transport processes occurring at the air-water interface of a liposome (lipid vesicle) solution are studied by conventional surface pressure-area measurements and interpreted by an adequate theory. At the interface of a dioleoyl phosphatidylcholine vesicle solution, used for demonstration, a well defined two layer structure selfassembles: vesicles disintegrate at the interface forming a surface-adsorbed lipid monolayer, which prevents further disintegration beyond about 1 dyne/cm surface pressure. A layer of vesicles now assembles in close association with the monolayer. This layer is in vesicle diffusion exchange with the solution and in lipid exchange with the monolayer. The lipid exchange occurs exclusively between the monolayer and the outer lipid layer of the vesicles; it is absent between outer and inner vesicle layers. Equilibration of the lipid density in the monolayer with that in the vesicle outer layer provides a coherent and quantitative explanation of the observed hysteresis effects and equilibrium states. The correspondence between monolayer and vesicle outer layer is traced down to equilibrium constants and rate constants and their dependences on surface pressure, vesicle size and concentration. Other alternate realizations of surface structure and exchange, including induced lipid flip-flop within vesicles or vesicle monolayer adhesion or fusion are potential applications of the proposed analysis."}
{"id": "PMID:476107", "title": "Sodium influx rate and ouabain-sensitive rubidium uptake in isolated guinea pig atria.", "content": "1. Ouabain-sensitive 86Rb+ uptake by tissue preparations has been used as an estimate of Na+ pump activity. This uptake, however, may be a measure of the Na+ influx rate, rather than capacity of the Na+ pump, since intracellular Na+ concentration is a determinant of the active Na+/Rb+ exchange reaction under certain conditions. This aspect was examined by studying the effect of altered Na+ influx rate on ouabain-sensitive 86Rb+ uptake in atrial preparations of guinea pig hearts. 2. Electrical stimulation markedly enhanced ouabain-sensitive 86Rb+ uptake without affecting nonspecific, ouabain-insensitive uptake. Paired-pulse stimulation studies indicate that the stimulation-induced enhancement of 86Rb+ uptake is due to membrane depolarizations, and hence related to the rate of Na+ influx. 3. Alterations in the extracellular Ca2+ concentration failed to affect the 86Rb+ uptake indicating that the force of contraction does not influence 86Rb+ uptake. 4. Reduced Na+ influx by low extracellular Na+ concentration decreased 86Rb+ uptake, and an increased Na+ influx by a Na+-specific ionophore, monensin, enhanced 86Rb+ uptake in quiescent atria. 5. Grayanotoxins, agents that increase transmembrane Na+ influx, and high concentrations of monensin appear to have inhibitory effects on ouabain-sensitive 86Rb+ uptake in electrically stimulated and in quiescent atria. 6. Electrical stimulation or monensin enhanced ouabain binding to (Na+ + K+)-ATPase and also increased the potency of ouabain to inhibit 86Rb+ uptake indicating that the intracellular Na+ available to the Na+ pump is increased under these conditions. 7. The ouabain-sensitive 86Rb+ uptake in electrically stimulated atria was less sensitive to alterations in the extracellular Na+ concentration, temperature and monensin than that in quiescent atria. 8. These results indicate that the rate of Na+ influx is the primary determinant of ouabain-sensitive 86Rb+ uptake in isolated atria. Electrical stimulation most effectively increases the Na+ available to the Na+ pump system. The ouabain-sensitive 86Rb+ uptake by atrial preparations under electrical stimulation at a relatively high frequency seems to represent the maximal capacity of the Na+ pump in this tissue.", "contents": "Sodium influx rate and ouabain-sensitive rubidium uptake in isolated guinea pig atria. 1. Ouabain-sensitive 86Rb+ uptake by tissue preparations has been used as an estimate of Na+ pump activity. This uptake, however, may be a measure of the Na+ influx rate, rather than capacity of the Na+ pump, since intracellular Na+ concentration is a determinant of the active Na+/Rb+ exchange reaction under certain conditions. This aspect was examined by studying the effect of altered Na+ influx rate on ouabain-sensitive 86Rb+ uptake in atrial preparations of guinea pig hearts. 2. Electrical stimulation markedly enhanced ouabain-sensitive 86Rb+ uptake without affecting nonspecific, ouabain-insensitive uptake. Paired-pulse stimulation studies indicate that the stimulation-induced enhancement of 86Rb+ uptake is due to membrane depolarizations, and hence related to the rate of Na+ influx. 3. Alterations in the extracellular Ca2+ concentration failed to affect the 86Rb+ uptake indicating that the force of contraction does not influence 86Rb+ uptake. 4. Reduced Na+ influx by low extracellular Na+ concentration decreased 86Rb+ uptake, and an increased Na+ influx by a Na+-specific ionophore, monensin, enhanced 86Rb+ uptake in quiescent atria. 5. Grayanotoxins, agents that increase transmembrane Na+ influx, and high concentrations of monensin appear to have inhibitory effects on ouabain-sensitive 86Rb+ uptake in electrically stimulated and in quiescent atria. 6. Electrical stimulation or monensin enhanced ouabain binding to (Na+ + K+)-ATPase and also increased the potency of ouabain to inhibit 86Rb+ uptake indicating that the intracellular Na+ available to the Na+ pump is increased under these conditions. 7. The ouabain-sensitive 86Rb+ uptake in electrically stimulated atria was less sensitive to alterations in the extracellular Na+ concentration, temperature and monensin than that in quiescent atria. 8. These results indicate that the rate of Na+ influx is the primary determinant of ouabain-sensitive 86Rb+ uptake in isolated atria. Electrical stimulation most effectively increases the Na+ available to the Na+ pump system. The ouabain-sensitive 86Rb+ uptake by atrial preparations under electrical stimulation at a relatively high frequency seems to represent the maximal capacity of the Na+ pump in this tissue."}
{"id": "PMID:476109", "title": "A high affinity site for sugar transport at the inner face of the human erythrocyte membrane?", "content": "A disagreement centering on a method of analysis as to the existence of a high affinity site for glucose transport at the inner face of the human red cell membrane is resolved by using direct fitting methods to confirm the original parameter estimates.", "contents": "A high affinity site for sugar transport at the inner face of the human erythrocyte membrane? A disagreement centering on a method of analysis as to the existence of a high affinity site for glucose transport at the inner face of the human red cell membrane is resolved by using direct fitting methods to confirm the original parameter estimates."}
{"id": "PMID:476110", "title": "Hypoglycemic drugs increase liver plasma membrane microviscosity in vitro.", "content": "The effect of hypoglycemic drugs chlorpropamide and phenformin has been tested on isolated liver plasma membranes as to their microviscosity parameters. Results reported suggest that both drugs are able to increase in vitro plasma membrane microviscosity in a dose-dependent way.", "contents": "Hypoglycemic drugs increase liver plasma membrane microviscosity in vitro. The effect of hypoglycemic drugs chlorpropamide and phenformin has been tested on isolated liver plasma membranes as to their microviscosity parameters. Results reported suggest that both drugs are able to increase in vitro plasma membrane microviscosity in a dose-dependent way."}
{"id": "PMID:476111", "title": "The structure of melittin in lipid bilayer membranes.", "content": "0.15 M inorganic phosphate dramatically increased the alpha-helix content of melittin in aqueous solution. When melittin interacted with egg yolk phosphatidylcholine liposomes in the absence of inorganic phosphate, it was converted to an alpha-helix rich form, as postulated by Dawson et al. (Dawson, C.R., Drake, A.F. Helliwell, J. and Hider, R.C. (1978) Biochim. Biophys. Acta 510, 75--86).", "contents": "The structure of melittin in lipid bilayer membranes. 0.15 M inorganic phosphate dramatically increased the alpha-helix content of melittin in aqueous solution. When melittin interacted with egg yolk phosphatidylcholine liposomes in the absence of inorganic phosphate, it was converted to an alpha-helix rich form, as postulated by Dawson et al. (Dawson, C.R., Drake, A.F. Helliwell, J. and Hider, R.C. (1978) Biochim. Biophys. Acta 510, 75--86)."}
{"id": "PMID:476112", "title": "Sodium dependence of neutral amino acid uptake into rabbit ileum.", "content": "Alanine uses two mediated pathways to enter the rabbit ileal mucosa. Present results suggest that one of them (Km = 4.1 mM) is fully dependent on sodium in the mucosal medium, while the other (Km = 91 mM) is sodium-independent. Similar results are obtained for methionine and serine. Reinterpretation of previous alanine/sodium coupling coefficients suggests that two sodium ions per alanine molecule are transported via the high affinity system.", "contents": "Sodium dependence of neutral amino acid uptake into rabbit ileum. Alanine uses two mediated pathways to enter the rabbit ileal mucosa. Present results suggest that one of them (Km = 4.1 mM) is fully dependent on sodium in the mucosal medium, while the other (Km = 91 mM) is sodium-independent. Similar results are obtained for methionine and serine. Reinterpretation of previous alanine/sodium coupling coefficients suggests that two sodium ions per alanine molecule are transported via the high affinity system."}
{"id": "PMID:476113", "title": "Complex phase mixing of phosphatidylcholine and phosphatidylserine in multilamellar membrane vesicles.", "content": "The phase mixing in dipalmitoyl phosphatidylcholine and bovine brain phosphatidylserine mixtures suspended in aqueous salt solutions was studied by differential scanning calorimetry, freeze-fracture electron microscopy and X-ray and electron diffraction. The pure dipalmitoyl phosphatidylcholine has two well-defined solidus phases P beta' and L beta' and a liquidus phase L alpha while the pure phosphatidylserine has a broad transition from L beta to L alpha. The mixture exhibits some dipalmitoyl phosphatidylcholine characteristics up to 30% phosphatidylserine when pure P beta' phase no longer exists. Phase mixing is observed at all compositions. An addition of 3% phosphatidylserine is found to be sufficient to destroy the sharp L beta'--P beta' transition observed in pure dipalmitoyl phosphatidylcholine. The transition between L beta' and P beta' in these mixtures is displaced to lower temperatures and becomes gradual as depicted by all three techniques, but freeze-fracture gives more definite information of the continuous transition. The most striking observation on the morphology of the mixed membranes (3--30% phosphatidylserine in dipalmitoyl phosphatidylcholine) is the presence of banded patterns (P beta') at temperatures well below the main transition peak as detected by differential scanning calorimetry.", "contents": "Complex phase mixing of phosphatidylcholine and phosphatidylserine in multilamellar membrane vesicles. The phase mixing in dipalmitoyl phosphatidylcholine and bovine brain phosphatidylserine mixtures suspended in aqueous salt solutions was studied by differential scanning calorimetry, freeze-fracture electron microscopy and X-ray and electron diffraction. The pure dipalmitoyl phosphatidylcholine has two well-defined solidus phases P beta' and L beta' and a liquidus phase L alpha while the pure phosphatidylserine has a broad transition from L beta to L alpha. The mixture exhibits some dipalmitoyl phosphatidylcholine characteristics up to 30% phosphatidylserine when pure P beta' phase no longer exists. Phase mixing is observed at all compositions. An addition of 3% phosphatidylserine is found to be sufficient to destroy the sharp L beta'--P beta' transition observed in pure dipalmitoyl phosphatidylcholine. The transition between L beta' and P beta' in these mixtures is displaced to lower temperatures and becomes gradual as depicted by all three techniques, but freeze-fracture gives more definite information of the continuous transition. The most striking observation on the morphology of the mixed membranes (3--30% phosphatidylserine in dipalmitoyl phosphatidylcholine) is the presence of banded patterns (P beta') at temperatures well below the main transition peak as detected by differential scanning calorimetry."}
{"id": "PMID:476114", "title": "Stimulation of monovalent cation fluxes by electron donors in the human red cell membrane.", "content": "When human red cells are incubated at 37 degrees C with the artificial electron donor system ascorbate + phenazine methosulphate the fluxes of Rb+ (K+) through the cell membrane are increased. The effect of this donor system is much stronger in energy-depleted than in normal cells. The same effects are produced by HS-glutathione, NADH or NADPH loaded into resealed ghosts, but these electron donors were ineffective when added to the incubation medium. The Rb+ (K+) fluxes induced by electron donors resemble closely those induced by an increase of intracellular Ca2+ (Gardos effect). The electron donors require the presence of intracellular Ca2+ to be effective, but at levels that do not stimulate by themselves the fluxes of K+. Flavoenzyme inhibitors (atebrin and chlorpromazine), oligomycin and quinine prevented the effects of both electron donors and Ca2+ alone; antimycin, upcouplers and ethacrynic acid inhibited them partially; ouabain, furosemide, and rotenone had no effect. The results could be explained if the effect of electron donors is to bring about a change in the redox state of some membrane component(s) that makes intracellular Ca2+ more effective to elicit rapid K+ movements. Plasma membrane oxidoreductase activities could be engaged in this change.", "contents": "Stimulation of monovalent cation fluxes by electron donors in the human red cell membrane. When human red cells are incubated at 37 degrees C with the artificial electron donor system ascorbate + phenazine methosulphate the fluxes of Rb+ (K+) through the cell membrane are increased. The effect of this donor system is much stronger in energy-depleted than in normal cells. The same effects are produced by HS-glutathione, NADH or NADPH loaded into resealed ghosts, but these electron donors were ineffective when added to the incubation medium. The Rb+ (K+) fluxes induced by electron donors resemble closely those induced by an increase of intracellular Ca2+ (Gardos effect). The electron donors require the presence of intracellular Ca2+ to be effective, but at levels that do not stimulate by themselves the fluxes of K+. Flavoenzyme inhibitors (atebrin and chlorpromazine), oligomycin and quinine prevented the effects of both electron donors and Ca2+ alone; antimycin, upcouplers and ethacrynic acid inhibited them partially; ouabain, furosemide, and rotenone had no effect. The results could be explained if the effect of electron donors is to bring about a change in the redox state of some membrane component(s) that makes intracellular Ca2+ more effective to elicit rapid K+ movements. Plasma membrane oxidoreductase activities could be engaged in this change."}
{"id": "PMID:476116", "title": "Lipid composition and microviscosity of subcellular fractions from rabbit thymocytes. Differences in the microviscosity of plasma membranes from subclasses of thymocytes.", "content": "There are indications from freeze-fracture experiments that subclasses of rabbit thymocytes show different mobilities of plasma membrane components. Consequently, one would expect differences in the fluidity of the plasma membrane. For this reason, rabbit thymocytes were separated on a Ficoll/Metrizoate gradient yielding three subclasses representing various levels of cell differentiation. These thymocyte subclasses did not show any significant differences in the degree of fluorescence polarization using the probe 1,6-diphenyl-1,3,5-hexatriene. The fluorescence polarization of the plasma membrane may be overshadowed by the contribution of all cellular lipids due to penetration of the fluorescent probe into the cell. Therefore, plasma membranes were isolated from rabbit thymocytes using a cell-disrupting pump, differential centrifugation, and sucrose density gradient centrifugation. As shown by biochemical and electron microscopical analyses, plasma membranes with a high degree of purity were obtained. As expected the plasma membrane fractions showed a higher microviscosity than the other subcellular fractions. This was attributed to a higher cholesterol to phospholipid molar ratio and a higher degree of saturation of phospholipid fatty acid chains. Subsequently, the microviscosity was measured of plasma membrane preparations obtained from two main subclasses of thymocytes representing mature and immature lymphocytes. The immature thymocytes yielded two plasma membrane fractions with higher microviscosity than the mature cells. These finding is in line with earlier observed differences in the glycerol-induced clustering of intramembranous particles. Furthermore, the results of this study support the view that the fluorescence polarization technique applied to whole cells does not exclusively monitor the plasma membrane.", "contents": "Lipid composition and microviscosity of subcellular fractions from rabbit thymocytes. Differences in the microviscosity of plasma membranes from subclasses of thymocytes. There are indications from freeze-fracture experiments that subclasses of rabbit thymocytes show different mobilities of plasma membrane components. Consequently, one would expect differences in the fluidity of the plasma membrane. For this reason, rabbit thymocytes were separated on a Ficoll/Metrizoate gradient yielding three subclasses representing various levels of cell differentiation. These thymocyte subclasses did not show any significant differences in the degree of fluorescence polarization using the probe 1,6-diphenyl-1,3,5-hexatriene. The fluorescence polarization of the plasma membrane may be overshadowed by the contribution of all cellular lipids due to penetration of the fluorescent probe into the cell. Therefore, plasma membranes were isolated from rabbit thymocytes using a cell-disrupting pump, differential centrifugation, and sucrose density gradient centrifugation. As shown by biochemical and electron microscopical analyses, plasma membranes with a high degree of purity were obtained. As expected the plasma membrane fractions showed a higher microviscosity than the other subcellular fractions. This was attributed to a higher cholesterol to phospholipid molar ratio and a higher degree of saturation of phospholipid fatty acid chains. Subsequently, the microviscosity was measured of plasma membrane preparations obtained from two main subclasses of thymocytes representing mature and immature lymphocytes. The immature thymocytes yielded two plasma membrane fractions with higher microviscosity than the mature cells. These finding is in line with earlier observed differences in the glycerol-induced clustering of intramembranous particles. Furthermore, the results of this study support the view that the fluorescence polarization technique applied to whole cells does not exclusively monitor the plasma membrane."}
{"id": "PMID:476115", "title": "Sodium gradient dependence of proline and glycine uptake in rat renal brush-border membrane vesicles.", "content": "The sodium-dependent entry of proline and glycine into rat renal brush-border membrane vesicles was examined. The high Km system for proline shows no sodium dependence. The low Km system for glycine entry is strictly dependent on a Na+ gradient but shows no evidence of the carrier system having any affinity for Na+. The low Km system for proline and high Km system for glycine transport appear to be shared. Both systems are stimulated by a Na+ gradient and appear to have an affinity for the Na+. The effect of decreasing the Na+ concentration in the ionic gradient is to alter the Km for amino acid entry and, at low Na+ concentrations, to inhibit the V for glycine entry.", "contents": "Sodium gradient dependence of proline and glycine uptake in rat renal brush-border membrane vesicles. The sodium-dependent entry of proline and glycine into rat renal brush-border membrane vesicles was examined. The high Km system for proline shows no sodium dependence. The low Km system for glycine entry is strictly dependent on a Na+ gradient but shows no evidence of the carrier system having any affinity for Na+. The low Km system for proline and high Km system for glycine transport appear to be shared. Both systems are stimulated by a Na+ gradient and appear to have an affinity for the Na+. The effect of decreasing the Na+ concentration in the ionic gradient is to alter the Km for amino acid entry and, at low Na+ concentrations, to inhibit the V for glycine entry."}
{"id": "PMID:476117", "title": "The calculated glucose concentration profile in the intercellular spaces of everted jejunum of rat.", "content": "On the basis of experimental data from isolated and everted rat jejunum and on extension of Diamond and Bossert's mathematical model, the glucose concentration in the intercellular spaces has been calculated and has been found higher (less than 2 mM) than that present in the serosal space.", "contents": "The calculated glucose concentration profile in the intercellular spaces of everted jejunum of rat. On the basis of experimental data from isolated and everted rat jejunum and on extension of Diamond and Bossert's mathematical model, the glucose concentration in the intercellular spaces has been calculated and has been found higher (less than 2 mM) than that present in the serosal space."}
{"id": "PMID:476118", "title": "Levels and distributions of phospholipids and cholesterol in the plasma membrane of neuroblastoma cells.", "content": "Murine neuroblastoma cells (clone N-2A) grown in suspension (spinner cells) or attached on a plastic surface (monolayer cells) were used in studies of the phospholipid and cholesterol composition of whole cells, primary plasma membranes, plasma membranes internalized during phagocytosis of polystyrene latex beads, mitochondria and microsomes. Monolayer cells contained higher concentrations of total phospholipid, phosphatidylserine and phosphatidylcholine, and lower concentration of phosphatidylethanolamine than spinner cells. The cholesterol levels and the relative proportions of the various phospholipids were similar in both cell types except phosphatidylethanolamine and sphingomyelin whose proportions were lower in monolayer cells. The primary plasma membranes of the two cell types differed significantly in the relative proportions of all phospholipids, except sphingomyelin, and the phospholipid to protein and the cholesterol to protein ratios were all higher in the membranes of spinner cells. In contrast to these results, all the phospholipid to protein and the cholesterol to protein ratios of the internalized plasma membranes were higher in monolayer than in spinner cells, and the proportions of all phospholipids, except phosphatidylethanolamine, were similar in both cell types. The membrane distributions of individual phospholipids and cholesterol were inferred from comparison of the phospholipid and cholesterol compositions of primary plasma membranes and plasma membranes internalized during phagocytosis of polystyrene beads. The results are consistent with a non-random distribution of most phospholipids in both spinner and monolayer cells, but the patterns of these distributions were different in the two cell types. With regard to cholesterol the results are compatible with a random or a heterogeneous distribution. All the phospholipid to protein ratios of the mitochondrial fraction of both cell types were lower than those of the plasma membranes. However, these ratios of the microsomal fraction were higher than those of the plasma membranes of monolayer cells, whereas they were comparable, with a few exceptions, to those of spinner cell membranes. The cholesterol to phospholipid molar ratios of plasma membranes were 6.4 and 4.3 fold greater than those of the mitochondrial and microsomal fractions, respectively.", "contents": "Levels and distributions of phospholipids and cholesterol in the plasma membrane of neuroblastoma cells. Murine neuroblastoma cells (clone N-2A) grown in suspension (spinner cells) or attached on a plastic surface (monolayer cells) were used in studies of the phospholipid and cholesterol composition of whole cells, primary plasma membranes, plasma membranes internalized during phagocytosis of polystyrene latex beads, mitochondria and microsomes. Monolayer cells contained higher concentrations of total phospholipid, phosphatidylserine and phosphatidylcholine, and lower concentration of phosphatidylethanolamine than spinner cells. The cholesterol levels and the relative proportions of the various phospholipids were similar in both cell types except phosphatidylethanolamine and sphingomyelin whose proportions were lower in monolayer cells. The primary plasma membranes of the two cell types differed significantly in the relative proportions of all phospholipids, except sphingomyelin, and the phospholipid to protein and the cholesterol to protein ratios were all higher in the membranes of spinner cells. In contrast to these results, all the phospholipid to protein and the cholesterol to protein ratios of the internalized plasma membranes were higher in monolayer than in spinner cells, and the proportions of all phospholipids, except phosphatidylethanolamine, were similar in both cell types. The membrane distributions of individual phospholipids and cholesterol were inferred from comparison of the phospholipid and cholesterol compositions of primary plasma membranes and plasma membranes internalized during phagocytosis of polystyrene beads. The results are consistent with a non-random distribution of most phospholipids in both spinner and monolayer cells, but the patterns of these distributions were different in the two cell types. With regard to cholesterol the results are compatible with a random or a heterogeneous distribution. All the phospholipid to protein ratios of the mitochondrial fraction of both cell types were lower than those of the plasma membranes. However, these ratios of the microsomal fraction were higher than those of the plasma membranes of monolayer cells, whereas they were comparable, with a few exceptions, to those of spinner cell membranes. The cholesterol to phospholipid molar ratios of plasma membranes were 6.4 and 4.3 fold greater than those of the mitochondrial and microsomal fractions, respectively."}
{"id": "PMID:476119", "title": "Synthesis and characterization of a new fluorescent phospholipid.", "content": "A novel fluorescent phospholipid, whose structure was tentatively assigned as 1-(2'-thio-1'-hydroxyethyl)-2-(ethylphosphatidyl)isoindole), was synthesized by reacting O-phthalaldehyde and beta-mercaptoethanol with phosphatidylethanolamine. The fluorescent lipid product was purified by silicic acid chromatography. The purity was demonstrated by thin-layer chromatography. This fluorescent phospholipid could not form stable lipid vesicles. However, a mixture of phosphatidylcholine and this fluorescent phospholipid did form stable vesicles after sonication, as demonstrated by Sepharose 4B column chromatography and electron microscopy. The absorption and fluorescence properties of this lipid, both as aqueous micelles or incorporated into vesicles, have been determined. The potential usage of this new fluorescent phospholipid in membrane studies is discussed.", "contents": "Synthesis and characterization of a new fluorescent phospholipid. A novel fluorescent phospholipid, whose structure was tentatively assigned as 1-(2'-thio-1'-hydroxyethyl)-2-(ethylphosphatidyl)isoindole), was synthesized by reacting O-phthalaldehyde and beta-mercaptoethanol with phosphatidylethanolamine. The fluorescent lipid product was purified by silicic acid chromatography. The purity was demonstrated by thin-layer chromatography. This fluorescent phospholipid could not form stable lipid vesicles. However, a mixture of phosphatidylcholine and this fluorescent phospholipid did form stable vesicles after sonication, as demonstrated by Sepharose 4B column chromatography and electron microscopy. The absorption and fluorescence properties of this lipid, both as aqueous micelles or incorporated into vesicles, have been determined. The potential usage of this new fluorescent phospholipid in membrane studies is discussed."}
{"id": "PMID:476120", "title": "Characteristics of phospholipids in microvillar membranes of octopus photoreceptor cells.", "content": "Characteristics of lipids in the microvillar membranes of octopus photoreceptor cells were studied in order to obtain some information on the membrane environment with rhodopsin in the invertebrate. (1) The membranes contain lipid and protein in almost equal proportion. The majority of lipids are phospholipids. Neutral lipids make up 16% of the total lipids, the major constituent of which is cholesterol. (2) Phosphatidylethanolamine and phosphatidylcholine are the major phospholipids. Phosphatidylserine, ceramide 2-aminoethylphosphonate and sphingomyelin occur as minor components. An unidentified alkaline and acid stable phospholipid was found. (3) The predominant fatty acids of phosphatidylethanolamine and phosphatidylcholine are highly unsaturated such as 22 : 6, 20 : 5 and 20 : 4. The 22 : 6 and 20 : 5 are exclusively linked at the 2-position, but the 20 : 4 is linked significantly at the 1-position of the phospholipids. (4) Major molecular species are 16 : 0/22 : 6 (48.4%) and 16 : 0/20 : 4 (19.6%) in phosphatidylcholine, and 20 : 4/22 : 6 (50.7%) and 16 : 0/22 : 6 (25.6%) in phosphatidylethanolamine.", "contents": "Characteristics of phospholipids in microvillar membranes of octopus photoreceptor cells. Characteristics of lipids in the microvillar membranes of octopus photoreceptor cells were studied in order to obtain some information on the membrane environment with rhodopsin in the invertebrate. (1) The membranes contain lipid and protein in almost equal proportion. The majority of lipids are phospholipids. Neutral lipids make up 16% of the total lipids, the major constituent of which is cholesterol. (2) Phosphatidylethanolamine and phosphatidylcholine are the major phospholipids. Phosphatidylserine, ceramide 2-aminoethylphosphonate and sphingomyelin occur as minor components. An unidentified alkaline and acid stable phospholipid was found. (3) The predominant fatty acids of phosphatidylethanolamine and phosphatidylcholine are highly unsaturated such as 22 : 6, 20 : 5 and 20 : 4. The 22 : 6 and 20 : 5 are exclusively linked at the 2-position, but the 20 : 4 is linked significantly at the 1-position of the phospholipids. (4) Major molecular species are 16 : 0/22 : 6 (48.4%) and 16 : 0/20 : 4 (19.6%) in phosphatidylcholine, and 20 : 4/22 : 6 (50.7%) and 16 : 0/22 : 6 (25.6%) in phosphatidylethanolamine."}
{"id": "PMID:476121", "title": "A theory of the electric field-induced phase transition of phospholipid bilayers.", "content": "Improving the statistical mechanical model of Jacobs et al. (Jacobs, R.E., Hudson, B. and Andersen, H.C. (1975) Proc. Natl. Acad. Sci. U.S. 72, 3993--3997) we have constructed a model which describes not only the temperature but also the external field dependence of the membrane structure of phospholipid bilayers. In addition to the interactions between head groups, between hydrocarbon chains, and the internal conformational energy of the chains (which were considered in Jacobs' model), our model includes the energy of deformation and the field energy as well. By the aid of this model we can explain the phenomenon of dielectric breakdown, the non-linearity of current-voltage characteristics, and the mechanism of membrane elasticity. The free energy of the membrane, the average number of the gauche conformations in the hydrocarbon interior and at the membrane surface, gauche distribution along the chain, the membrane thickness, area and volume are calculated at different temperatures and voltages. The calculation also gives the temperature dependence of Young's modulus and that of the linear thermal expansion coefficient.", "contents": "A theory of the electric field-induced phase transition of phospholipid bilayers. Improving the statistical mechanical model of Jacobs et al. (Jacobs, R.E., Hudson, B. and Andersen, H.C. (1975) Proc. Natl. Acad. Sci. U.S. 72, 3993--3997) we have constructed a model which describes not only the temperature but also the external field dependence of the membrane structure of phospholipid bilayers. In addition to the interactions between head groups, between hydrocarbon chains, and the internal conformational energy of the chains (which were considered in Jacobs' model), our model includes the energy of deformation and the field energy as well. By the aid of this model we can explain the phenomenon of dielectric breakdown, the non-linearity of current-voltage characteristics, and the mechanism of membrane elasticity. The free energy of the membrane, the average number of the gauche conformations in the hydrocarbon interior and at the membrane surface, gauche distribution along the chain, the membrane thickness, area and volume are calculated at different temperatures and voltages. The calculation also gives the temperature dependence of Young's modulus and that of the linear thermal expansion coefficient."}
{"id": "PMID:476123", "title": "Phosphonic and arsonic acids as inhibitors of human red cell acid phosphatase and their use in affinity chromatography.", "content": "1. In order to obtain an effective ligand for affinity chromatography of the low molecular weight acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) from human red cells nine phosphonic and two arsonic acid substrate analogues were investigated as potential inhibitors. The two forms of acid phosphatase type B (b1 and b2) were isolated and partially purified using conventional methods and the inhibitory action of the substrate analogs investigated. 2. Four of the phosphonic acids were relatively effective competitive inhibitors. It appears that certain structural and electronic requirements have to be fulfilled by the phosphonic acids in order to exhibit significant affinity for the enzyme. A high affinity appears to require the presence of a bulky, hydrophobic moiety which has to be separated from the phosphorus atom by the distance of one atom. 3. p-Aminobenzylphosphonic acid exerted the highest affinity for acid phosphatase with a pH optimum at 6.5. Ki values of 4 . 10(-4) and 6 . 10(-4) M were found for the b1 and b2 forms, respectively. 4. Coupling of p-aminobenzylphosphonic acid to Agarose yielded an effective and specific affinity medium. By means of affinity chromatography using this medium, acid phosphatase was purified 500-fold in a single step.", "contents": "Phosphonic and arsonic acids as inhibitors of human red cell acid phosphatase and their use in affinity chromatography. 1. In order to obtain an effective ligand for affinity chromatography of the low molecular weight acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) from human red cells nine phosphonic and two arsonic acid substrate analogues were investigated as potential inhibitors. The two forms of acid phosphatase type B (b1 and b2) were isolated and partially purified using conventional methods and the inhibitory action of the substrate analogs investigated. 2. Four of the phosphonic acids were relatively effective competitive inhibitors. It appears that certain structural and electronic requirements have to be fulfilled by the phosphonic acids in order to exhibit significant affinity for the enzyme. A high affinity appears to require the presence of a bulky, hydrophobic moiety which has to be separated from the phosphorus atom by the distance of one atom. 3. p-Aminobenzylphosphonic acid exerted the highest affinity for acid phosphatase with a pH optimum at 6.5. Ki values of 4 . 10(-4) and 6 . 10(-4) M were found for the b1 and b2 forms, respectively. 4. Coupling of p-aminobenzylphosphonic acid to Agarose yielded an effective and specific affinity medium. By means of affinity chromatography using this medium, acid phosphatase was purified 500-fold in a single step."}
{"id": "PMID:476124", "title": "Post-proline cleaving enzyme. Synthesis of a new fluorogenic substrate and distribution of the endopeptidase in rat tissues and body fluids of man.", "content": "Synthesis and application of the first fluorogenic substrate, N-carbobenzoxyglycylprolyl-4-methylcoumarinyl amide (Z-Gly-Pro-MeCouNH) for the determination of the post-proline cleaving enzyme (EC 3.4.21.-) were reported. Maximal activity of the enzyme purified from lamb kidney for the new substrate was observed at pH 7.0. This substrate showed a higher affinity (Km = 0.02 mM) for the enzyme than the proline containing substrates studied previously and allowed the detection of 10-50 ng post-proline cleaving enzyme activity per ml sample after a 1 min incubation period. Distribution of post-proline cleaving enzyme and other proline specific peptidases in rat tissues was studied using Z-Gly-Pro-MeCouNH and other proline-containing substrates. High post-proline cleaving enzyme activity was observed in testis, liver and skeletal muscle. Inhibition experiments indicated that post-proline cleaving enzyme activity was completely inactivated by 0.1 mM diisopropylphosphofluoridate and Z-Gly-Pro-chloromethylketone, as had been found in the case of the enzyme isolated from lamb kidney. Activity in human body fluids was also tested for levels of post-proline cleaving enzyme activity using Z-Gly-Pro-MeCouNH and semen was found to show the highest cleaving activity.", "contents": "Post-proline cleaving enzyme. Synthesis of a new fluorogenic substrate and distribution of the endopeptidase in rat tissues and body fluids of man. Synthesis and application of the first fluorogenic substrate, N-carbobenzoxyglycylprolyl-4-methylcoumarinyl amide (Z-Gly-Pro-MeCouNH) for the determination of the post-proline cleaving enzyme (EC 3.4.21.-) were reported. Maximal activity of the enzyme purified from lamb kidney for the new substrate was observed at pH 7.0. This substrate showed a higher affinity (Km = 0.02 mM) for the enzyme than the proline containing substrates studied previously and allowed the detection of 10-50 ng post-proline cleaving enzyme activity per ml sample after a 1 min incubation period. Distribution of post-proline cleaving enzyme and other proline specific peptidases in rat tissues was studied using Z-Gly-Pro-MeCouNH and other proline-containing substrates. High post-proline cleaving enzyme activity was observed in testis, liver and skeletal muscle. Inhibition experiments indicated that post-proline cleaving enzyme activity was completely inactivated by 0.1 mM diisopropylphosphofluoridate and Z-Gly-Pro-chloromethylketone, as had been found in the case of the enzyme isolated from lamb kidney. Activity in human body fluids was also tested for levels of post-proline cleaving enzyme activity using Z-Gly-Pro-MeCouNH and semen was found to show the highest cleaving activity."}
{"id": "PMID:476125", "title": "A purification and some properties of two proteases from papaya latex.", "content": "Two proteases, one of which is papaya peptidase A and the other a previously unknown enzyme in papaya latex have been purified to homogeneity in a simple two stage process. Both are markedly less reactive than papain or chymopapain. Each has a molecular weight of 24,000, N-terminal sequences commencing Leu-Pro-Glu, and contains no carbohydrate. Their amino acid compositions differ for several residues. The essential -SH groups of the enzymes examined appear to be 'masked' in the native state.", "contents": "A purification and some properties of two proteases from papaya latex. Two proteases, one of which is papaya peptidase A and the other a previously unknown enzyme in papaya latex have been purified to homogeneity in a simple two stage process. Both are markedly less reactive than papain or chymopapain. Each has a molecular weight of 24,000, N-terminal sequences commencing Leu-Pro-Glu, and contains no carbohydrate. Their amino acid compositions differ for several residues. The essential -SH groups of the enzymes examined appear to be 'masked' in the native state."}
{"id": "PMID:476126", "title": "Isolation of completely inactive plasma prorenin and its activation by kallikreins. A possible new link between renin and kallikrein.", "content": "Existing views on prorenin are conflicting and its physiological activation mechanism is not clear. In an attempt to obtain clearcut views on the molecular properties of prorenin in human plasma, the renin zymogen (prorenin) was separated from active renin by two steps of affinity chromatography and it was demonstrated that prorenin is a completely inactivate zymogen contrary to the existing information. Inactive prorenin has an apparent molecular of 56,000 contrary to 46,000-43,000 of partially active prorenin. Isolated and acid-treated human prorenin was shown to be activated by kallikreins from human urine and plasma. This activation was completely blocked by Trasylol. Hog pancreatic kallikrein also activated human prorenin. The kallikrein mediated activation of prorenin indicates the existence of a new link between the vasoconstricting renin-angiotensin system and the vasodilating kallikreinkinin system.", "contents": "Isolation of completely inactive plasma prorenin and its activation by kallikreins. A possible new link between renin and kallikrein. Existing views on prorenin are conflicting and its physiological activation mechanism is not clear. In an attempt to obtain clearcut views on the molecular properties of prorenin in human plasma, the renin zymogen (prorenin) was separated from active renin by two steps of affinity chromatography and it was demonstrated that prorenin is a completely inactivate zymogen contrary to the existing information. Inactive prorenin has an apparent molecular of 56,000 contrary to 46,000-43,000 of partially active prorenin. Isolated and acid-treated human prorenin was shown to be activated by kallikreins from human urine and plasma. This activation was completely blocked by Trasylol. Hog pancreatic kallikrein also activated human prorenin. The kallikrein mediated activation of prorenin indicates the existence of a new link between the vasoconstricting renin-angiotensin system and the vasodilating kallikreinkinin system."}
{"id": "PMID:476127", "title": "Fluoride inhibition of inorganic pyrophosphatase. IV. Evidence for metal participation in the active center and a four-site model of metal effect on catalysis.", "content": "Atomic spectroscopy of native yeast inorganic pyrophosphatase (pyrophosphate phosphohydrolase, EC 3.6.1.1) after gel filtration showed that it only binds activating Mg2% in an easily dissociable manner. Formation of a covalent intermediate between the enzyme and an entire substrate molecular in the presence of fluoride, however, dramatically strengthened the binding of two Mg2+ per subunit and eliminated at neutral pH the effect of added metals on protein fluorescence but not on the absorption spectrum, suggesting that different mental binding sites influence the two spectra. This conclusion was confirmed by spectra studied on native enzyme. A third, low-affinity site for Mg2+ was found on the enzyme pH greater than 8. A model of enzyme-substrate-metal interactions was proposed, according to which the fluorescence-controlling site belongs to the active center and substrate can only be bound to it as a 1 : 1 complex with metals.", "contents": "Fluoride inhibition of inorganic pyrophosphatase. IV. Evidence for metal participation in the active center and a four-site model of metal effect on catalysis. Atomic spectroscopy of native yeast inorganic pyrophosphatase (pyrophosphate phosphohydrolase, EC 3.6.1.1) after gel filtration showed that it only binds activating Mg2% in an easily dissociable manner. Formation of a covalent intermediate between the enzyme and an entire substrate molecular in the presence of fluoride, however, dramatically strengthened the binding of two Mg2+ per subunit and eliminated at neutral pH the effect of added metals on protein fluorescence but not on the absorption spectrum, suggesting that different mental binding sites influence the two spectra. This conclusion was confirmed by spectra studied on native enzyme. A third, low-affinity site for Mg2+ was found on the enzyme pH greater than 8. A model of enzyme-substrate-metal interactions was proposed, according to which the fluorescence-controlling site belongs to the active center and substrate can only be bound to it as a 1 : 1 complex with metals."}
{"id": "PMID:476129", "title": "Mechanism of action of iodothyronine-5'-deiodinase.", "content": "Production of 3,3'-di-iodothyronine (3,3'-T2) from 3,3',5'-tri-iodothyronine (reverse T3, rT3) as catalysed by rat liver microsomal fraction was measured with a specific radioimmunoassay. The effect of the addition of 2-thiouracil and of varying concentrations of cofactor (dithiothreito) on the kinetic parameters of this reaction were studied. It was found that thiouracil is an uncompetitive inhibitor with respect to substrate and a competitive inhibitor with respect to cofactor. The effect of a decrease in the concentration of cofactor was similar to the effect of addition of thiouracil, i.e. a proportional decrease in Km and V. The results strongly suggest that enzymatic 5'-deiodination of iodothyronines follows a ping-pong mechanisms, which may be envisaged as a transiodination and the subsequent reduction of the iodo-enzyme complex by cofactor. The intermediate is probably a sulfenyl iodide form of the enzyme, which reacts with thiouracil to yield a mixed disulfide.", "contents": "Mechanism of action of iodothyronine-5'-deiodinase. Production of 3,3'-di-iodothyronine (3,3'-T2) from 3,3',5'-tri-iodothyronine (reverse T3, rT3) as catalysed by rat liver microsomal fraction was measured with a specific radioimmunoassay. The effect of the addition of 2-thiouracil and of varying concentrations of cofactor (dithiothreito) on the kinetic parameters of this reaction were studied. It was found that thiouracil is an uncompetitive inhibitor with respect to substrate and a competitive inhibitor with respect to cofactor. The effect of a decrease in the concentration of cofactor was similar to the effect of addition of thiouracil, i.e. a proportional decrease in Km and V. The results strongly suggest that enzymatic 5'-deiodination of iodothyronines follows a ping-pong mechanisms, which may be envisaged as a transiodination and the subsequent reduction of the iodo-enzyme complex by cofactor. The intermediate is probably a sulfenyl iodide form of the enzyme, which reacts with thiouracil to yield a mixed disulfide."}
{"id": "PMID:476128", "title": "A study of the reaction catalysed by alginate lyase VI from the sea mollusc, Littorina sp.", "content": "The molecular weight of polymeric alginic acid digested by alginate lyase (poly(1,4-beta-D-mannuronide) lyase, EC 4.2.2.3) was determined at various stages of the lysis. Low molecular weigh fragments were detected only after 60-100% lysis. Some high molecular weight fragments remained intact even after addition of a fresh aliquot of enzyme to the digest. The enzyme showed maximal activity at pH 5.6 in 0.05 M salt. Enzyme activity was stimulated by addition of 7.5 mM CaCl2 and 0.2 M NaCl, when the pH optimum was between 8 and 8.5. Only mannuronic acid was detected at the reducing end of fragments after exhausive enzymolysis, reduction and hydrolysis. On studying the reaction products by NMR, a double-bound signal (sigma = 5.98 ppm) was observed. A considerable decrease in intensity of the D-mannuronic acid residue signal was detected after hydrolysis of alginate lyase VI on poly-(ManUA-GulUA), but not poly(GulUA). The results suggest that alginate lyase VI may be an endoalginate lyase that splits glycoside bonds only between two mannuronic acid residues.", "contents": "A study of the reaction catalysed by alginate lyase VI from the sea mollusc, Littorina sp. The molecular weight of polymeric alginic acid digested by alginate lyase (poly(1,4-beta-D-mannuronide) lyase, EC 4.2.2.3) was determined at various stages of the lysis. Low molecular weigh fragments were detected only after 60-100% lysis. Some high molecular weight fragments remained intact even after addition of a fresh aliquot of enzyme to the digest. The enzyme showed maximal activity at pH 5.6 in 0.05 M salt. Enzyme activity was stimulated by addition of 7.5 mM CaCl2 and 0.2 M NaCl, when the pH optimum was between 8 and 8.5. Only mannuronic acid was detected at the reducing end of fragments after exhausive enzymolysis, reduction and hydrolysis. On studying the reaction products by NMR, a double-bound signal (sigma = 5.98 ppm) was observed. A considerable decrease in intensity of the D-mannuronic acid residue signal was detected after hydrolysis of alginate lyase VI on poly-(ManUA-GulUA), but not poly(GulUA). The results suggest that alginate lyase VI may be an endoalginate lyase that splits glycoside bonds only between two mannuronic acid residues."}
{"id": "PMID:476130", "title": "The oxidation and decarboxylation of retinoic acid by horseradish peroxidase.", "content": "The decarboxylation of retinoic acid by horseradish peroxidase was investigated. A marked increase in the yield of products was obtained. However, the data indicated the reaction was a nonenzymatic, heme catalyzed peroxidation. Previously reported requirements for phosphate, oxygen and ferrous ion were eliminated when hydrogen peroxide was provided. Peroxide also eliminated the EDTA and cyanide induced inhibition of the phosphate dependent system. In the presence of hydrogen peroxide, horseradish peroxidase was not essential to the reaction; heme equivalent amounts of hemoglobin decarboxylated retinoic acid with equal facility. However, hemoglobin was ineffective in the absence of hydrogen peroxide. Attainment of 50--60% decarboxylation represented complete utilization of the available retinoic acid. Thus the products of the reaction can be divided into two groups, products of retinoic acid oxidation and products of an oxidative decarboxylation of retinoic acid.", "contents": "The oxidation and decarboxylation of retinoic acid by horseradish peroxidase. The decarboxylation of retinoic acid by horseradish peroxidase was investigated. A marked increase in the yield of products was obtained. However, the data indicated the reaction was a nonenzymatic, heme catalyzed peroxidation. Previously reported requirements for phosphate, oxygen and ferrous ion were eliminated when hydrogen peroxide was provided. Peroxide also eliminated the EDTA and cyanide induced inhibition of the phosphate dependent system. In the presence of hydrogen peroxide, horseradish peroxidase was not essential to the reaction; heme equivalent amounts of hemoglobin decarboxylated retinoic acid with equal facility. However, hemoglobin was ineffective in the absence of hydrogen peroxide. Attainment of 50--60% decarboxylation represented complete utilization of the available retinoic acid. Thus the products of the reaction can be divided into two groups, products of retinoic acid oxidation and products of an oxidative decarboxylation of retinoic acid."}
{"id": "PMID:476131", "title": "Purification and properties of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.", "content": "3-Hydroxy-3-methylglutaryl coenzyme A reductase has been purified from rat liver microsomes with a recovery of approx. 25%. The enzyme was homogeneous on gel electrophoresis and enzyme activity comigrated  with the single protein band. The molecular weight of the reductase determined by gel filtration on Sephadex G-200 was 200,000. SDS-polyacrylamide gel electrophoresis gave a subunit molecular weight of 52,000 +/- 2000, suggesting that the enzyme was a tetramer. The specific activities of the purified enzyme obtained from rats fed diets containing 0% or 5% cholestyramine were 11,303 and 19,584 nmol NADPH oxidized/min per mg protein, respectively. The reductase showed unique binding properties to Cibacron Blue Sepharose; the enzyme was bound to the Cibacron Blue via the binding sites for both substrates, NADPH and (S)-3-hydroxy-3-methylglutaryl coenzyme A. Antibodies prepared against purified reductase inactivated 100% of the soluble and at least 91% of the microsomal enzyme activity. Immunotitrations of solubilized enzyme obtained from normal and cholestyramine-fed rats indicated that cholestyramine feeding both increased the amount of enzyme protein and resulted in enzyme activation. Administration of increasing amounts of mevalonolactone to rats decreased the equivalence point obtained from immunotitration studies with solubilized enzyme. These data indicate that the antibody cross-reacts with the inactive enzyme formed after mevalonolactone treatment.", "contents": "Purification and properties of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase. 3-Hydroxy-3-methylglutaryl coenzyme A reductase has been purified from rat liver microsomes with a recovery of approx. 25%. The enzyme was homogeneous on gel electrophoresis and enzyme activity comigrated  with the single protein band. The molecular weight of the reductase determined by gel filtration on Sephadex G-200 was 200,000. SDS-polyacrylamide gel electrophoresis gave a subunit molecular weight of 52,000 +/- 2000, suggesting that the enzyme was a tetramer. The specific activities of the purified enzyme obtained from rats fed diets containing 0% or 5% cholestyramine were 11,303 and 19,584 nmol NADPH oxidized/min per mg protein, respectively. The reductase showed unique binding properties to Cibacron Blue Sepharose; the enzyme was bound to the Cibacron Blue via the binding sites for both substrates, NADPH and (S)-3-hydroxy-3-methylglutaryl coenzyme A. Antibodies prepared against purified reductase inactivated 100% of the soluble and at least 91% of the microsomal enzyme activity. Immunotitrations of solubilized enzyme obtained from normal and cholestyramine-fed rats indicated that cholestyramine feeding both increased the amount of enzyme protein and resulted in enzyme activation. Administration of increasing amounts of mevalonolactone to rats decreased the equivalence point obtained from immunotitration studies with solubilized enzyme. These data indicate that the antibody cross-reacts with the inactive enzyme formed after mevalonolactone treatment."}
{"id": "PMID:476132", "title": "Lysozyme sensitivity of pantothenate-deficient Lactobacillus plantarum grown with exogenous fatty acids.", "content": "A pantothenic acid deficiency in Lactobacillus plantarum reduces lipid synthesis, prevents normal uptake and retention of extracellular amino acids and markedly increases sensitivity of these cells to lysozyme induced lysis. Pantothenate-deficient cells provided with exogenous fatty acids synthesize additional lipids and express nearly normal solute transport activities. The present study has shown that such cells retain a heightened sensitivity to lysozyme induced lysis. These observations indicate that the lysozyme sensitivity of pantothenate-deficient cells is not produced as in indirect effect of membrane lipid depletion, but represents an independent consequence of pantothenate insufficiency.", "contents": "Lysozyme sensitivity of pantothenate-deficient Lactobacillus plantarum grown with exogenous fatty acids. A pantothenic acid deficiency in Lactobacillus plantarum reduces lipid synthesis, prevents normal uptake and retention of extracellular amino acids and markedly increases sensitivity of these cells to lysozyme induced lysis. Pantothenate-deficient cells provided with exogenous fatty acids synthesize additional lipids and express nearly normal solute transport activities. The present study has shown that such cells retain a heightened sensitivity to lysozyme induced lysis. These observations indicate that the lysozyme sensitivity of pantothenate-deficient cells is not produced as in indirect effect of membrane lipid depletion, but represents an independent consequence of pantothenate insufficiency."}
{"id": "PMID:476133", "title": "Factors regulating the elongation of palmitic and stearic acid by rat liver microsomes.", "content": "Analysis of the rates of overall chain elongation and condensation of malonyl-CoA with palmitoyl-CoA and stearoyl-CoA as primers demonstrated that for each primer, the rate of the overall metabolic process was similar to the initial condensation. The specific activity for condensation with palmitoyl-CoA was eleven times greater than for stearoyl-CoA. The specific activities of both the beta-hydroxyacyl-CoA dehydrase and 2-trans-enoyl-CoA reductase reactions were much higher than for either condensation or chain elongation, although these rates were somewhat greater with the intermediates required in chain elongating palmitoyl-CoA than for stearoyl-CoA. Both substrates were incorporated into phospholipids at low rates and there was a time-dependent hydrolytic cleavage of the acyl-CoA primers which was partially prevented by bovine serum albumin. These findings demonstrate that there was no selective removal of either primer which could result in specific substrate depletion and an apparent reduction in the rate of condensation. These combined results firmly establish the rate-limiting nature and high degree of substrate specificity exhibited during the initial condensation step in fatty acid elongation.", "contents": "Factors regulating the elongation of palmitic and stearic acid by rat liver microsomes. Analysis of the rates of overall chain elongation and condensation of malonyl-CoA with palmitoyl-CoA and stearoyl-CoA as primers demonstrated that for each primer, the rate of the overall metabolic process was similar to the initial condensation. The specific activity for condensation with palmitoyl-CoA was eleven times greater than for stearoyl-CoA. The specific activities of both the beta-hydroxyacyl-CoA dehydrase and 2-trans-enoyl-CoA reductase reactions were much higher than for either condensation or chain elongation, although these rates were somewhat greater with the intermediates required in chain elongating palmitoyl-CoA than for stearoyl-CoA. Both substrates were incorporated into phospholipids at low rates and there was a time-dependent hydrolytic cleavage of the acyl-CoA primers which was partially prevented by bovine serum albumin. These findings demonstrate that there was no selective removal of either primer which could result in specific substrate depletion and an apparent reduction in the rate of condensation. These combined results firmly establish the rate-limiting nature and high degree of substrate specificity exhibited during the initial condensation step in fatty acid elongation."}
{"id": "PMID:476134", "title": "Origin of the arachidonic acid released post-mortem in rat forebrain.", "content": "To determine the origins of the arachidonic acid released post-mortem in brain tissue, [3H]arachidonic acid was injected by the intracerebro-ventricular route and radioactivity monitored in complex lipids and free arachidonic acid at various times after decapitation. The specific activity of the released arachidonic acid was close to that in the total phospholipid fraction and much lower than that of the neutral lipids. The phospholipid with the closest specific activity to the free arachidonic acid recovered at the end of the post-mortem period was phosphatidylinositol. Phosphatidylcholine showed a small but significant decrease in radioactivity post-mortem and could contribute 37% of the arachidonic acid released to the free fatty acid fraction. Arachidonic acid released in rat forebrain after decapitation thus comes from a mixture of phospholipids with phosphatidylinositol and phosphatidylcholine being the major source. Phosphatidylserine and phosphatidic acid did not make important contributions to the free arachidonic acid. In the microsomal fraction, the specific activity of the free arachidonic acid was very close to that in phosphatidylinositol.", "contents": "Origin of the arachidonic acid released post-mortem in rat forebrain. To determine the origins of the arachidonic acid released post-mortem in brain tissue, [3H]arachidonic acid was injected by the intracerebro-ventricular route and radioactivity monitored in complex lipids and free arachidonic acid at various times after decapitation. The specific activity of the released arachidonic acid was close to that in the total phospholipid fraction and much lower than that of the neutral lipids. The phospholipid with the closest specific activity to the free arachidonic acid recovered at the end of the post-mortem period was phosphatidylinositol. Phosphatidylcholine showed a small but significant decrease in radioactivity post-mortem and could contribute 37% of the arachidonic acid released to the free fatty acid fraction. Arachidonic acid released in rat forebrain after decapitation thus comes from a mixture of phospholipids with phosphatidylinositol and phosphatidylcholine being the major source. Phosphatidylserine and phosphatidic acid did not make important contributions to the free arachidonic acid. In the microsomal fraction, the specific activity of the free arachidonic acid was very close to that in phosphatidylinositol."}
{"id": "PMID:476135", "title": "Metabolism of prostaglandin E1 and of glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1) by prostaglandin 9-ketoreductase from rabbit kidney.", "content": "Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1). Apparent Km (GSH-prostaglandin A1) 13 microM and apparent Km (prostaglandin E1) 200 microM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E1 and GSH-prostaglandin A1 occurs by means of the same fractions. Furthermore, prostaglandin E1 and GSH-prostaglandin A1 are competitive inhibitors of the enzyme, when GSH-prostaglandin A1 and prostaglandin E1 are tested as substrates, respectively. It si concluded, that GSH-prostaglandin A1 is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E1.", "contents": "Metabolism of prostaglandin E1 and of glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1) by prostaglandin 9-ketoreductase from rabbit kidney. Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1). Apparent Km (GSH-prostaglandin A1) 13 microM and apparent Km (prostaglandin E1) 200 microM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E1 and GSH-prostaglandin A1 occurs by means of the same fractions. Furthermore, prostaglandin E1 and GSH-prostaglandin A1 are competitive inhibitors of the enzyme, when GSH-prostaglandin A1 and prostaglandin E1 are tested as substrates, respectively. It si concluded, that GSH-prostaglandin A1 is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E1."}
{"id": "PMID:476136", "title": "Plasma membrane lipids of human diploid fibroblasts from normal individuals and patients with cystic fibrosis.", "content": "The lipid composition of isolated plasma membranes of human skin fibroblasts is described for the first time. Plasma membranes from a number of strains of fibroblasts from patients with cystic fibrosis and matched normals were isolated by a recently described procedure and analysed for major phospholipid classes, cholesterol and fatty acids. No differences in the quantities of these compounds were detected between cells of the two different origins. The fetal calf serum used to supplement the growth medium contained relatively more palmitoleate and oleate but less stearate than the membranes. There were also no consistent differences between cystic fibrosis and normal membranes in terms of the fatty acid compositions of their individual phospholipid classes. Consistent with this lack of chemical change in the lipids of membranes of cystic fibrosis cells, the degree of fluorescence polarization of diphenylhexatriene, an index of fluidity, was also unchanged.", "contents": "Plasma membrane lipids of human diploid fibroblasts from normal individuals and patients with cystic fibrosis. The lipid composition of isolated plasma membranes of human skin fibroblasts is described for the first time. Plasma membranes from a number of strains of fibroblasts from patients with cystic fibrosis and matched normals were isolated by a recently described procedure and analysed for major phospholipid classes, cholesterol and fatty acids. No differences in the quantities of these compounds were detected between cells of the two different origins. The fetal calf serum used to supplement the growth medium contained relatively more palmitoleate and oleate but less stearate than the membranes. There were also no consistent differences between cystic fibrosis and normal membranes in terms of the fatty acid compositions of their individual phospholipid classes. Consistent with this lack of chemical change in the lipids of membranes of cystic fibrosis cells, the degree of fluorescence polarization of diphenylhexatriene, an index of fluidity, was also unchanged."}
{"id": "PMID:476137", "title": "Studies on molecular species of choline glycerophospholipids of developing rat brain.", "content": "The chronological changes in molecular species of choline glycerophospholipids were studied for cerebra of 17-, 19- and 21-day-old rat fetuses, and 3-, 6-, 12-, 24- and 90-day-old rats. The molecular species found by gas chromatography-mass spectrometry and selected ion retrieval technique were phosphatidylcholines of '30 : 0, 32 : 0, 32 : 1, 34 : 0, 34 : 1, 34 : 2, 36 : 0, 36 : 1, 36 : 2, 36 : 3, and 36 : 4' where the larger number indicates the sum of chain lengths on positions C-1 and C-2; the smaller number is the total number of double bonds. Of these molecular species, '32 : 0' (mainly 16 : 0/16 : 0, dipalmitoyl glycerophosphorylcholine), '34 : 1' (mainly 16 : 0/18 : 1, palmitoyloleoyl glycerophosphorylcholine), '34 : 0' (16 : 0/18 : 0, palmitoylstearoyl glycerophosphorylcholine), '32 : 1' (mainly 16 : 0/16 : 1, palmitoylpalmitoleoyl glycerophosphorylcholine and '30 : 0' (14 : 0/16 : 0, myristoylpalmitoyl glycerophosphorylcholine) were main species. The '32 : 0' species increased to about 44% at around the 10th day and thereafter remained nearly constant. '34 : 1' and '34 : 0' decreased to about 17 and 6% at that time and then increased to about 30 and 14%, respectively. '30 : 0' increased from last stage of gestation to the 6th day and then decreased. '32 : 1' was about 16% for 17-day-old fetus and decreased grandually. '36 : 1' (18 : 0/18 : 1, stearoyloleoyl glycerophosphorylcholine) increased at the latter part of development.", "contents": "Studies on molecular species of choline glycerophospholipids of developing rat brain. The chronological changes in molecular species of choline glycerophospholipids were studied for cerebra of 17-, 19- and 21-day-old rat fetuses, and 3-, 6-, 12-, 24- and 90-day-old rats. The molecular species found by gas chromatography-mass spectrometry and selected ion retrieval technique were phosphatidylcholines of '30 : 0, 32 : 0, 32 : 1, 34 : 0, 34 : 1, 34 : 2, 36 : 0, 36 : 1, 36 : 2, 36 : 3, and 36 : 4' where the larger number indicates the sum of chain lengths on positions C-1 and C-2; the smaller number is the total number of double bonds. Of these molecular species, '32 : 0' (mainly 16 : 0/16 : 0, dipalmitoyl glycerophosphorylcholine), '34 : 1' (mainly 16 : 0/18 : 1, palmitoyloleoyl glycerophosphorylcholine), '34 : 0' (16 : 0/18 : 0, palmitoylstearoyl glycerophosphorylcholine), '32 : 1' (mainly 16 : 0/16 : 1, palmitoylpalmitoleoyl glycerophosphorylcholine and '30 : 0' (14 : 0/16 : 0, myristoylpalmitoyl glycerophosphorylcholine) were main species. The '32 : 0' species increased to about 44% at around the 10th day and thereafter remained nearly constant. '34 : 1' and '34 : 0' decreased to about 17 and 6% at that time and then increased to about 30 and 14%, respectively. '30 : 0' increased from last stage of gestation to the 6th day and then decreased. '32 : 1' was about 16% for 17-day-old fetus and decreased grandually. '36 : 1' (18 : 0/18 : 1, stearoyloleoyl glycerophosphorylcholine) increased at the latter part of development."}
{"id": "PMID:476138", "title": "A facile permethylation of cord factor.", "content": "Small quantities of cord factor (trehalose-6,6'-dimycolates) can be readily and almost quantitatively permethylated in anhydrous diethyl ether/dimethylformamide mixtures with CH3I and NaH-oil dispersion in the presence of molecular sieve. Hydrolysis of the permethylated products from cord factor \"Peurois\" and 'P3-Aoyama-b' prove these to be 6,6'-dimycolates. Experiments with a 4-palmitoyl glucose suggest that acyl migration in this system may not occur.", "contents": "A facile permethylation of cord factor. Small quantities of cord factor (trehalose-6,6'-dimycolates) can be readily and almost quantitatively permethylated in anhydrous diethyl ether/dimethylformamide mixtures with CH3I and NaH-oil dispersion in the presence of molecular sieve. Hydrolysis of the permethylated products from cord factor \"Peurois\" and 'P3-Aoyama-b' prove these to be 6,6'-dimycolates. Experiments with a 4-palmitoyl glucose suggest that acyl migration in this system may not occur."}
{"id": "PMID:476139", "title": "Positional distribution of exogenous and endogenous fatty acids in triacylglycerols formed by rat adipocytes in vitro.", "content": "Rat adipocytes were used in vitro to compare the positional distributions of fatty acids of intra- and extra-cellular origin in triacyl-sn-glycerols. Fatty acids of extracellular origin were esterified to each position in similar, but not identical, proportions to the natural distributions. A high proportion of the oleic acid synthesised in the tissue by desaturation of exogenous stearic acid was found in position sn-3. When palmitic acid was the only fatty acid added, tripalmitoylglycerol was synthesised by the adipocytes. The rate and pattern of fatty acids synthesised de novo from acetate was dependent on the age of the donor rat and the concentration of acetate and presence or absence of long-chain fatty acids in the medium. The newly synthesised fatty acids were esterified in very different proportions from the natural distributions and thus from those of extracellular fatty acids. The results are discussed in terms of esterification of the fatty acids from the two sources in different compartments of the cell.", "contents": "Positional distribution of exogenous and endogenous fatty acids in triacylglycerols formed by rat adipocytes in vitro. Rat adipocytes were used in vitro to compare the positional distributions of fatty acids of intra- and extra-cellular origin in triacyl-sn-glycerols. Fatty acids of extracellular origin were esterified to each position in similar, but not identical, proportions to the natural distributions. A high proportion of the oleic acid synthesised in the tissue by desaturation of exogenous stearic acid was found in position sn-3. When palmitic acid was the only fatty acid added, tripalmitoylglycerol was synthesised by the adipocytes. The rate and pattern of fatty acids synthesised de novo from acetate was dependent on the age of the donor rat and the concentration of acetate and presence or absence of long-chain fatty acids in the medium. The newly synthesised fatty acids were esterified in very different proportions from the natural distributions and thus from those of extracellular fatty acids. The results are discussed in terms of esterification of the fatty acids from the two sources in different compartments of the cell."}
{"id": "PMID:476140", "title": "Isolation and structure of diglycosylsterols and triglycosylsterols in rice bran.", "content": "Novel oligoglycosylsterols, a series of sterylglycosides, were detected in the glycolipids of rice bran, and di- and triglycosylsterols were isolated and their structures were investigated. The principal sterol components were sitosterol, campesterol and stigmasterol; glucose was the only component sugar. The major species of diglycosylsterol and triglycosylsterol were characterized as D-glucopyranosyl-(beta 1 leads to 4)-D-glucopyranosyl-(beta 1 leads to 3')-sitosterol, i.e. cellobiosylsitosterol, and D-glucopyranosyl-(beta 1 leads to 4)-D-glucopyranosyl-(beta 1 leads to 4)-D-glucopyranosyl-(beta 1 leads to 3')-sitosterol, i.e. cellotriosylsitosterol, respectively.", "contents": "Isolation and structure of diglycosylsterols and triglycosylsterols in rice bran. Novel oligoglycosylsterols, a series of sterylglycosides, were detected in the glycolipids of rice bran, and di- and triglycosylsterols were isolated and their structures were investigated. The principal sterol components were sitosterol, campesterol and stigmasterol; glucose was the only component sugar. The major species of diglycosylsterol and triglycosylsterol were characterized as D-glucopyranosyl-(beta 1 leads to 4)-D-glucopyranosyl-(beta 1 leads to 3')-sitosterol, i.e. cellobiosylsitosterol, and D-glucopyranosyl-(beta 1 leads to 4)-D-glucopyranosyl-(beta 1 leads to 4)-D-glucopyranosyl-(beta 1 leads to 3')-sitosterol, i.e. cellotriosylsitosterol, respectively."}
{"id": "PMID:476141", "title": "Pyruvate cycling involving possible oxaloacetate decarboxylase activity.", "content": "With high concentrations of pyruvate as substrate for hepatocytes from fasted rats, high rates of cycling between pyruvate and the dicarboxylic acids occur, as shown isotopically. This rate of cycling is adequate to account for the hydrogen translocation from the mitochondria to the cytosol to furnish NADH for lactate formation. Addition of sufficiently high concentrations of mercaptopicolinate to block almost completely glucose formation from pyruvate, depresses isotopic cycling and lactate formation by only about 50-75%. Under some conditions, when the normal phosphoenolpyruvate carboxykinase activity is inhibited, cytosolic oxaloacetate may be decarboxylated directly to pyruvate, possibly via the decarboxylase activity of phosphoenolpyruvate carboxykinase.", "contents": "Pyruvate cycling involving possible oxaloacetate decarboxylase activity. With high concentrations of pyruvate as substrate for hepatocytes from fasted rats, high rates of cycling between pyruvate and the dicarboxylic acids occur, as shown isotopically. This rate of cycling is adequate to account for the hydrogen translocation from the mitochondria to the cytosol to furnish NADH for lactate formation. Addition of sufficiently high concentrations of mercaptopicolinate to block almost completely glucose formation from pyruvate, depresses isotopic cycling and lactate formation by only about 50-75%. Under some conditions, when the normal phosphoenolpyruvate carboxykinase activity is inhibited, cytosolic oxaloacetate may be decarboxylated directly to pyruvate, possibly via the decarboxylase activity of phosphoenolpyruvate carboxykinase."}
{"id": "PMID:476144", "title": "Quantitative assay of types I and III collagen synthesized by keloid biopsyes and fibroblasts.", "content": "Molecular sieve column chromatography was used to determine the amount of type I and III collagen synthesized by normal dermis and keloid biopsies and fibroblasts derived from these tissues. After incubation with radioactive proline, the collagen was extracted and separated into types I and III and then quantitated. There was no significant difference in the percent type III collagen synthesized by fresh keloid biopsies compared to normal dermis. Likewise, there was no significant difference in the percent type III collagen synthesized by keloid fibroblasts compared to normal dermal fibroblasts. However, fibroblasts from both keloid and normal dermis synthesized a lower percentage of type III collagen in cell culture compared to the original biopsies. These findings demonstrate that keloid collagen has the same type distribution as normal dermis and suggest that increased collagen synthesis in these lesions is not related to altered collagen types.", "contents": "Quantitative assay of types I and III collagen synthesized by keloid biopsyes and fibroblasts. Molecular sieve column chromatography was used to determine the amount of type I and III collagen synthesized by normal dermis and keloid biopsies and fibroblasts derived from these tissues. After incubation with radioactive proline, the collagen was extracted and separated into types I and III and then quantitated. There was no significant difference in the percent type III collagen synthesized by fresh keloid biopsies compared to normal dermis. Likewise, there was no significant difference in the percent type III collagen synthesized by keloid fibroblasts compared to normal dermal fibroblasts. However, fibroblasts from both keloid and normal dermis synthesized a lower percentage of type III collagen in cell culture compared to the original biopsies. These findings demonstrate that keloid collagen has the same type distribution as normal dermis and suggest that increased collagen synthesis in these lesions is not related to altered collagen types."}
{"id": "PMID:476145", "title": "Age-related effects on the incorporation of acetate into rat liver histones.", "content": "Incorporation of sodium [3H]acetate into histones of rats was examined as a function of age. Incorporation was observed to decline with age up to 24 months, at which time a levelling occurred. Controls indicated that this decrease in histone acetylation could not be attributed to variability in isotope delivery to the liver or to alterations in intracellular 'pools' available for acetylation. Polyacrylamide gel electrophoresis established that, in all cases, acetate was incorporated primarily into histone fractions H3 and H4 and the pattern of incorporation exhibited age-dependent phenomena. H4 was predominantly labelled in 2 months animals, while in 12, 16, and 24 months animals H3 was more highly labelled; at 27 months the two fractions were labelled equally. Assessment of histone acetylase and deacetylase activities indicates that deacetylase activity increased with age.", "contents": "Age-related effects on the incorporation of acetate into rat liver histones. Incorporation of sodium [3H]acetate into histones of rats was examined as a function of age. Incorporation was observed to decline with age up to 24 months, at which time a levelling occurred. Controls indicated that this decrease in histone acetylation could not be attributed to variability in isotope delivery to the liver or to alterations in intracellular 'pools' available for acetylation. Polyacrylamide gel electrophoresis established that, in all cases, acetate was incorporated primarily into histone fractions H3 and H4 and the pattern of incorporation exhibited age-dependent phenomena. H4 was predominantly labelled in 2 months animals, while in 12, 16, and 24 months animals H3 was more highly labelled; at 27 months the two fractions were labelled equally. Assessment of histone acetylase and deacetylase activities indicates that deacetylase activity increased with age."}
{"id": "PMID:476147", "title": "Partition behaviour of a cultured mouse mammary cancer cell line in aqueous two-phase polymer systems.", "content": "Cell surface-associated changes in behaviour of cultured cells on partition in an aqueous two-phase polymer system were studied using FM3A cell line (a cultured mammary cancer of mouse) with respect to aging. The aqueous polymer system consisted of dextran, polyethyleneglycol and sodium phosphate, equilibrated at 6 degrees C to separate into two phases. Enzyme treatment of cells with neuraminidase reduced cell electrophoretic mobility, as well as the cell partition ratio. Hyaluronidase produced no observable effects on partition and cell electrophoretic mobility, suggesting that the partition is related to culture time was similar for both cell electrophoretic mobility and cell partition, showing a rise and fall of charge-associated cell surface change during cell growth, the maxium occurring at the beginning of exponential growth. This change was reflected in the pattern of countercurrent distribution of the cells in respective stages of growth. Countercurrent distribution with our two-phase system is expected to be capable of fractionating cell populations according to cell surface properties.", "contents": "Partition behaviour of a cultured mouse mammary cancer cell line in aqueous two-phase polymer systems. Cell surface-associated changes in behaviour of cultured cells on partition in an aqueous two-phase polymer system were studied using FM3A cell line (a cultured mammary cancer of mouse) with respect to aging. The aqueous polymer system consisted of dextran, polyethyleneglycol and sodium phosphate, equilibrated at 6 degrees C to separate into two phases. Enzyme treatment of cells with neuraminidase reduced cell electrophoretic mobility, as well as the cell partition ratio. Hyaluronidase produced no observable effects on partition and cell electrophoretic mobility, suggesting that the partition is related to culture time was similar for both cell electrophoretic mobility and cell partition, showing a rise and fall of charge-associated cell surface change during cell growth, the maxium occurring at the beginning of exponential growth. This change was reflected in the pattern of countercurrent distribution of the cells in respective stages of growth. Countercurrent distribution with our two-phase system is expected to be capable of fractionating cell populations according to cell surface properties."}
{"id": "PMID:476148", "title": "Secretion and uptake of beta-N-acetylglucosaminidase by fibroblasts. Effect of chloroquine and mannose 6-phosphate.", "content": "The effects of chloroquine and mannose 6-hosphate on the secretion and uptake of the lysosomal enzyme, beta-N-acetylglucosaminidase (EC 3.2.1.30), by human fibroblasts have been compared. There was a reciprocal relationship between intracellular depletion, and extracellular accumulation, of enzyme at chloroquine concentrations ranging from 5 micrometers to 100 micrometers. A loss of enzyme activity from the system (intra- plus extracellular activity) with increasing concentrations of chloroquine was due to inhibition of the beta-N-acetylglucosaminidase. At a concentration of 50 micrometers, chloroquine elicited a three fold increase in the extracellular accumulation of beta-N-acetylglucosaminidase in 24 h whereas the addition of 5 micrometers mannose 6-phosphate (a competitive inhibitor of receptor-mediated uptake) resulted in only a 13% increase. Uptake of beta-N-acetylglucosaminidase by enzyme-deficient fibroblasts was completely inhibited by 5 micrometers mannose 6-phosphate. In the presence of chloroquine there was also no uptake of enzyme, however ther was a marked decrease in the residual activity of the cells. The results suggest that the effect of chloroquine on fibroblasts is to stimulate secretion rather than to inhibit uptake as previously reported. The isoenzyme pattern of the beta-N-acetylglucosaminidase from normal culture medium was compared with that accumulating in the medium following exposure of the cells to 50 micrometers chloroquine. In the presence of chloroquine, there was an increase in the A isoenzyme, however the activity was eluted in a broad peak which probably represents several closely related forms of the enzyme. There was an almost total loss of the A isoenzyme of beta-N-acetylglucosaminidase from fibroblasts cultured in the presence of chloroquine. A small peak of activity eluting at a similar position to the secreted, As, isoenzyme was present in extracts of chloroquine-treated fibroblasts, suggesting that the As isoenzyme is formed and/or stored at a site distinct from the intracellular isoenzyme.", "contents": "Secretion and uptake of beta-N-acetylglucosaminidase by fibroblasts. Effect of chloroquine and mannose 6-phosphate. The effects of chloroquine and mannose 6-hosphate on the secretion and uptake of the lysosomal enzyme, beta-N-acetylglucosaminidase (EC 3.2.1.30), by human fibroblasts have been compared. There was a reciprocal relationship between intracellular depletion, and extracellular accumulation, of enzyme at chloroquine concentrations ranging from 5 micrometers to 100 micrometers. A loss of enzyme activity from the system (intra- plus extracellular activity) with increasing concentrations of chloroquine was due to inhibition of the beta-N-acetylglucosaminidase. At a concentration of 50 micrometers, chloroquine elicited a three fold increase in the extracellular accumulation of beta-N-acetylglucosaminidase in 24 h whereas the addition of 5 micrometers mannose 6-phosphate (a competitive inhibitor of receptor-mediated uptake) resulted in only a 13% increase. Uptake of beta-N-acetylglucosaminidase by enzyme-deficient fibroblasts was completely inhibited by 5 micrometers mannose 6-phosphate. In the presence of chloroquine there was also no uptake of enzyme, however ther was a marked decrease in the residual activity of the cells. The results suggest that the effect of chloroquine on fibroblasts is to stimulate secretion rather than to inhibit uptake as previously reported. The isoenzyme pattern of the beta-N-acetylglucosaminidase from normal culture medium was compared with that accumulating in the medium following exposure of the cells to 50 micrometers chloroquine. In the presence of chloroquine, there was an increase in the A isoenzyme, however the activity was eluted in a broad peak which probably represents several closely related forms of the enzyme. There was an almost total loss of the A isoenzyme of beta-N-acetylglucosaminidase from fibroblasts cultured in the presence of chloroquine. A small peak of activity eluting at a similar position to the secreted, As, isoenzyme was present in extracts of chloroquine-treated fibroblasts, suggesting that the As isoenzyme is formed and/or stored at a site distinct from the intracellular isoenzyme."}
{"id": "PMID:476149", "title": "Stimulation of de novo synthesis of L-phenylalanine ammonia-lyase in relation to phytoalexin accumulation in Colletotrichum lindemuthianum elicitor-treated cell suspension cultures of french bean (Phaseolus vulgaris).", "content": "(1) The regulation of the accumulation of the isoflavonoid-derived phytoalexin phaseollin in cell suspension cultures of Dwarf French Bean (Phaseolus vulgaris/ has been investigated. (2) An elicitor preparation from cell walls of Colletotrichum lindemuthianum, the causal agent of anthracnose disease of French bean, caused a marked accumulation of phaseollin in the cultures. The elicitor induced phaseollin accumulation to a level of 60% that obtained with the artificial elicitor autoclaved ribonuclease A and was maximally active at a concentration (weight basis) of at least 50 times lower than required for maximal response to ribonuclease. (3) Elicitor preparations from cell walls of Phytophthora megasperma var. sojae, a fungal pathogen of soybean, and Botrytis cinerea, the common grey mould, were much less effective than the C. lindemuthianum wall-released elicitor. (4) There was a marked but transient increase in the extractable activity of phenylalanine ammonia-lyase, the enzyme catalysing the first reaction in the biosynthesis of phaseollin from L-phenylalanine, in response to the elicitor from C. lindemuthianum. (5) Comparative density labelling with 2H from 2H2O indicated that the elicitor stimulates de novo synthesis of phenylalanine ammonie findings provide the basis of a scheme for elicitor induction of phytoalexin accumulation.", "contents": "Stimulation of de novo synthesis of L-phenylalanine ammonia-lyase in relation to phytoalexin accumulation in Colletotrichum lindemuthianum elicitor-treated cell suspension cultures of french bean (Phaseolus vulgaris). (1) The regulation of the accumulation of the isoflavonoid-derived phytoalexin phaseollin in cell suspension cultures of Dwarf French Bean (Phaseolus vulgaris/ has been investigated. (2) An elicitor preparation from cell walls of Colletotrichum lindemuthianum, the causal agent of anthracnose disease of French bean, caused a marked accumulation of phaseollin in the cultures. The elicitor induced phaseollin accumulation to a level of 60% that obtained with the artificial elicitor autoclaved ribonuclease A and was maximally active at a concentration (weight basis) of at least 50 times lower than required for maximal response to ribonuclease. (3) Elicitor preparations from cell walls of Phytophthora megasperma var. sojae, a fungal pathogen of soybean, and Botrytis cinerea, the common grey mould, were much less effective than the C. lindemuthianum wall-released elicitor. (4) There was a marked but transient increase in the extractable activity of phenylalanine ammonia-lyase, the enzyme catalysing the first reaction in the biosynthesis of phaseollin from L-phenylalanine, in response to the elicitor from C. lindemuthianum. (5) Comparative density labelling with 2H from 2H2O indicated that the elicitor stimulates de novo synthesis of phenylalanine ammonie findings provide the basis of a scheme for elicitor induction of phytoalexin accumulation."}
{"id": "PMID:476150", "title": "Absorption, serum levels and urinary excretion of inorganic sulfate after oral administration of sodium sulfate in the conscious rat.", "content": "The absorption of inorganic sulfate after ingestion was investigated in rats. After oral administration of Na235SO4, 35S radioactivity was measurable in plasma already after 15 min and its plasma concentration reached a peak after about 1.5--2 h. The 35S-radioactivity excreted in urine during 24 h after ingestion of Na235SO4 together with varying amounts of unlabelled Na2SO4 (0.25--5.0 mmol Na2SO4 per rat) indicated an almost complete absorption of inorganic sulfate from the gastrointestinal tract. Determination of the inorganic sulfate concentration in rat serum 2 h after oral administration of 5.0 mmol Na2SO4 revealed a three-fold increase in serum sulfate concentration. The data suggest a rapid and almost complete absorption of inorganic sulfate after oral administration in the rat. Its importance in relation to the sulfate availability for sulfate conjugation of drugs is discussed.", "contents": "Absorption, serum levels and urinary excretion of inorganic sulfate after oral administration of sodium sulfate in the conscious rat. The absorption of inorganic sulfate after ingestion was investigated in rats. After oral administration of Na235SO4, 35S radioactivity was measurable in plasma already after 15 min and its plasma concentration reached a peak after about 1.5--2 h. The 35S-radioactivity excreted in urine during 24 h after ingestion of Na235SO4 together with varying amounts of unlabelled Na2SO4 (0.25--5.0 mmol Na2SO4 per rat) indicated an almost complete absorption of inorganic sulfate from the gastrointestinal tract. Determination of the inorganic sulfate concentration in rat serum 2 h after oral administration of 5.0 mmol Na2SO4 revealed a three-fold increase in serum sulfate concentration. The data suggest a rapid and almost complete absorption of inorganic sulfate after oral administration in the rat. Its importance in relation to the sulfate availability for sulfate conjugation of drugs is discussed."}
{"id": "PMID:476152", "title": "Lipid carriers in the synthesis of high-mannose glycoproteins in algae.", "content": "Particulate preparations from the chlorophyta Prototheca zopfii catalyze the incorporation of mannose and N-acetylglucosamine into glycolipids. These had been characterized as lipid monophosphate mannose, lipid pyrophosphate N,N'-diacetylchitobiose and various lipid-linked oligosaccharides containing two N-acetylglucosamine residues plus a variable number of mannose residues. The lipid moiety has the properties expected for dolichyl phosphate. The oligosacchride-linked lipids serve as precursors for the formation of a polymer sensible to pronase digestion. The oligosaccharide is linked by N-glycosidic linkage to an asparagine residue. In longer incubation periods, a polymer insensitive to pronase hydrolysis, but precipitable by copper salts such as cell wall mannans is formed. Polymer formation is inhibited by 1 mM bacitracin. The reactions leading to the formation of the mannoprotein were found associated to the rough endoplasmic reticulum. The synthesis of mannans was found to occur in the Golgi vesicles.", "contents": "Lipid carriers in the synthesis of high-mannose glycoproteins in algae. Particulate preparations from the chlorophyta Prototheca zopfii catalyze the incorporation of mannose and N-acetylglucosamine into glycolipids. These had been characterized as lipid monophosphate mannose, lipid pyrophosphate N,N'-diacetylchitobiose and various lipid-linked oligosaccharides containing two N-acetylglucosamine residues plus a variable number of mannose residues. The lipid moiety has the properties expected for dolichyl phosphate. The oligosacchride-linked lipids serve as precursors for the formation of a polymer sensible to pronase digestion. The oligosaccharide is linked by N-glycosidic linkage to an asparagine residue. In longer incubation periods, a polymer insensitive to pronase hydrolysis, but precipitable by copper salts such as cell wall mannans is formed. Polymer formation is inhibited by 1 mM bacitracin. The reactions leading to the formation of the mannoprotein were found associated to the rough endoplasmic reticulum. The synthesis of mannans was found to occur in the Golgi vesicles."}
{"id": "PMID:476151", "title": "ADP-ribosylation of membrane proteins and activation of adenylate cyclase by cholera toxin in fat cell ghosts from euthyroid and hypothyroid rats.", "content": "Incubation of fat cell ghosts with activated cholera toxin, nucleoside triphosphate, cytosol, and NAD results in increased adenylate cyclase activity and the transfer of ADP-ribose to membrane proteins. The major ADP-ribose protein comigrates on sodium dodecyl sulfate-polyacrylamide gels with the putative GTP-binding protein of pigeon erythrocyte membranes (Mr 42 000), which is also ADP-ribosylated by cholera toxin. The treatment with cholera toxin enhances the stimulation of the fat cell membrane adenylate cyclase by GTP, but the stimulation by guanyl-5'-yl imidodiphosphate is unaltered. Subsequent stimulation of fat cell adenylate cyclase by 10 micrometers epinephrine is not particularly affected. These changes were qualititatively the same for membranes isolated from fat cells of hypothyroid rats. Although the cyclase of these membranes has a reduced response to epinephrine, guanyl-5'-yl imidodiphosphate or GTP, as compared to euthyroid rat fat cell membranes, the defect is not rectified by toxin treatment and cannot be explained by a deficiency in the cholera toxin target.", "contents": "ADP-ribosylation of membrane proteins and activation of adenylate cyclase by cholera toxin in fat cell ghosts from euthyroid and hypothyroid rats. Incubation of fat cell ghosts with activated cholera toxin, nucleoside triphosphate, cytosol, and NAD results in increased adenylate cyclase activity and the transfer of ADP-ribose to membrane proteins. The major ADP-ribose protein comigrates on sodium dodecyl sulfate-polyacrylamide gels with the putative GTP-binding protein of pigeon erythrocyte membranes (Mr 42 000), which is also ADP-ribosylated by cholera toxin. The treatment with cholera toxin enhances the stimulation of the fat cell membrane adenylate cyclase by GTP, but the stimulation by guanyl-5'-yl imidodiphosphate is unaltered. Subsequent stimulation of fat cell adenylate cyclase by 10 micrometers epinephrine is not particularly affected. These changes were qualititatively the same for membranes isolated from fat cells of hypothyroid rats. Although the cyclase of these membranes has a reduced response to epinephrine, guanyl-5'-yl imidodiphosphate or GTP, as compared to euthyroid rat fat cell membranes, the defect is not rectified by toxin treatment and cannot be explained by a deficiency in the cholera toxin target."}
{"id": "PMID:476153", "title": "Epinephrine and glucagon counteract inhibition of protein synthesis induced by D-galactosamine in isolated mouse hepatocytes.", "content": "10 mM D-galactosamine enhibited protein synthesis (1 h incubation time) by 67% in isolated mouse liver cells. Counteracting uridylate deficiency induced by D-galactosamine by preventive administration of 20 mM uridine did not decrease the extent of protein synthesis inhibition. 20 mM D-galactose reverted the inhibition of protein synthesis by D-galactosamine. 10(-5) M epinephrine and 10(-7) M glucagon decreased the incorporation of D-galactosamine into glycogen to 38% and 26% of the control value, respectively, after a 35 min incubation and reduced the inhibition of protein synthesis by D-galactosamine effectively. Experimental evidence supports the view that aminoglycogen formed after D-galactosamine treatment is responsible for the inhibition of protein synthesis.", "contents": "Epinephrine and glucagon counteract inhibition of protein synthesis induced by D-galactosamine in isolated mouse hepatocytes. 10 mM D-galactosamine enhibited protein synthesis (1 h incubation time) by 67% in isolated mouse liver cells. Counteracting uridylate deficiency induced by D-galactosamine by preventive administration of 20 mM uridine did not decrease the extent of protein synthesis inhibition. 20 mM D-galactose reverted the inhibition of protein synthesis by D-galactosamine. 10(-5) M epinephrine and 10(-7) M glucagon decreased the incorporation of D-galactosamine into glycogen to 38% and 26% of the control value, respectively, after a 35 min incubation and reduced the inhibition of protein synthesis by D-galactosamine effectively. Experimental evidence supports the view that aminoglycogen formed after D-galactosamine treatment is responsible for the inhibition of protein synthesis."}
{"id": "PMID:476154", "title": "Ultracentrifugation evidence for a somatostatin-binding protein in serum.", "content": "Using a technique of high speed centrifugation of serum and a well validated immunoassay for the measurement of serum somatostatin-like immunoreactivity, we have demonstrated that somatostatin, unlike other peptide hormones, appears to sediment with large molecular weight proteins. When synthetic somatostatin of increasing concentration was incubated with serum prior to ultracentrifugation, a linear plot of concentration of somatostatin added against concentration sedimenting (or apparently bound to protein) revealed an association curve. These data provide further evidence for the existence of a serum-binding protein for somatostatin.", "contents": "Ultracentrifugation evidence for a somatostatin-binding protein in serum. Using a technique of high speed centrifugation of serum and a well validated immunoassay for the measurement of serum somatostatin-like immunoreactivity, we have demonstrated that somatostatin, unlike other peptide hormones, appears to sediment with large molecular weight proteins. When synthetic somatostatin of increasing concentration was incubated with serum prior to ultracentrifugation, a linear plot of concentration of somatostatin added against concentration sedimenting (or apparently bound to protein) revealed an association curve. These data provide further evidence for the existence of a serum-binding protein for somatostatin."}
{"id": "PMID:476155", "title": "Interaction of lectins with human platelets. Effects on platelet stimulation by thrombin and ristocetin.", "content": "Wheat germ agglutinin induced aggregation and secretion of fresh platelets. Aggregation, but not secretion of serotonin by platelets in plasma, by the lectin was inhibited by 5 mM EDTA. Further, the lectin-induced stimulation of fresh platelets was blocked by prostaglandin E1. Thus, this lectin stimulates platelets by a mechanism which closely mimics thrombin activation and is independent of intercellular crosslinking. Lentil lectin did not stimulate platelets. Each platelet contained about 6 . 10(-5) binding sites for the lectins with an apparent dissociation constant of 3.0 . 10(-7) M. Wheat germ agglutinin, which binds mainly to glycoprotein I (Mr 150 000), increased the subsequent binding of thrombin to fixed platelets while lentil lectin was without effect. It appears that thrombin and wheat germ agglutinin bind to independent but interacting sites. Wheat germ agglutinin, but neither thrombin nor lentil lectin, inhibited the agglutination of platelets by ristocetin. Further, rat platelets were not aggregated by either ristocetin or wheat germ agglutinin. It appears that the interaction sites of ristocetin and wheat germ agglutinin on platelets are overlapping.", "contents": "Interaction of lectins with human platelets. Effects on platelet stimulation by thrombin and ristocetin. Wheat germ agglutinin induced aggregation and secretion of fresh platelets. Aggregation, but not secretion of serotonin by platelets in plasma, by the lectin was inhibited by 5 mM EDTA. Further, the lectin-induced stimulation of fresh platelets was blocked by prostaglandin E1. Thus, this lectin stimulates platelets by a mechanism which closely mimics thrombin activation and is independent of intercellular crosslinking. Lentil lectin did not stimulate platelets. Each platelet contained about 6 . 10(-5) binding sites for the lectins with an apparent dissociation constant of 3.0 . 10(-7) M. Wheat germ agglutinin, which binds mainly to glycoprotein I (Mr 150 000), increased the subsequent binding of thrombin to fixed platelets while lentil lectin was without effect. It appears that thrombin and wheat germ agglutinin bind to independent but interacting sites. Wheat germ agglutinin, but neither thrombin nor lentil lectin, inhibited the agglutination of platelets by ristocetin. Further, rat platelets were not aggregated by either ristocetin or wheat germ agglutinin. It appears that the interaction sites of ristocetin and wheat germ agglutinin on platelets are overlapping."}
{"id": "PMID:476156", "title": "Evidence for a plasma inhibitor of the heparin accelerated inhibition of factor Xa by antithrombin III.", "content": "The ability of heparin fractions of different molecular weight to potentiate the action of antithrombin III against the coagulation factors thrombin and Xa has been examined in purified reaction mixtures and in plasma. Residual thrombin and Xa have been determined by their peptidase activities against the synthetic peptide substrates H-D-Phe-Pip-Arg-pNA and Bz-Ile-Gly-Arg-pNA. High molecular weight heparin fractions were found to have higher anticoagulant activities than low molecular weight heparin when studied with both thrombin and Xa incubation mixtures in purified mixtures and in plasma. The inhibition of thrombin by heparin fractions and antithrombin III was unaffected by other plasma components. However, normal human plasma contained a component that inhibited the heparin and antithrombin III inhibition of Xa particularly when the high molecular weight heparin fraction was used. Experiments using a purified preparation of platelet factor 4 suggested that the platelet-derived heparin-neutralizing protein was not responsible for the inhibition.", "contents": "Evidence for a plasma inhibitor of the heparin accelerated inhibition of factor Xa by antithrombin III. The ability of heparin fractions of different molecular weight to potentiate the action of antithrombin III against the coagulation factors thrombin and Xa has been examined in purified reaction mixtures and in plasma. Residual thrombin and Xa have been determined by their peptidase activities against the synthetic peptide substrates H-D-Phe-Pip-Arg-pNA and Bz-Ile-Gly-Arg-pNA. High molecular weight heparin fractions were found to have higher anticoagulant activities than low molecular weight heparin when studied with both thrombin and Xa incubation mixtures in purified mixtures and in plasma. The inhibition of thrombin by heparin fractions and antithrombin III was unaffected by other plasma components. However, normal human plasma contained a component that inhibited the heparin and antithrombin III inhibition of Xa particularly when the high molecular weight heparin fraction was used. Experiments using a purified preparation of platelet factor 4 suggested that the platelet-derived heparin-neutralizing protein was not responsible for the inhibition."}
{"id": "PMID:476157", "title": "Estrogen-dependent and progesterone-arrested synthesis and secretion of sulfated glycoproteins in luminal epithelia of rat uteri.", "content": "1. 35SO4 administered intraperitoneally was specifically incorporated into a glycopeptide component separated by electrophoresis of the glycosaminoglycan fraction prepared from the uterine epithelia (luminal), as well as the uterine fluid of ovariectomized rats treated with estradiol-17 beta, in contrast to the rats without estrogen treatment. 2. The epithelial cells of uteri isolated from estrogen-treated ovariectomized rats incorporated 35SO4 in vitro into at least two macromolecular components. The larger molecular weight component (sodium dodecyl sulfate polyacryl-amide gel electrophoresis and/or gel filtration) labelled with 35S was observed in both the cytosol and particulate fractions, whereas the smaller molecular weight component labelled with 35S was found only in the particulate fraction. 35SO4 was also incorporated into two macromolecular components in the incubation medium, similarly to the particulate fraction. A 35 SO4-labelled glycopeptide similar to that from the epithelial particulate fraction and the incubation medium, and not from the epithelial cytosol fraction. 3. Progesterone, in contrast to estrogen, did not stimulate the sulfated blycoprotein synthesis. Moreover, progesterone administered together with or after estrogen-administration completely arrested the estrogen-dependent synthesis and secretion of the sulfated glycoproteins in the uterine epithelia.", "contents": "Estrogen-dependent and progesterone-arrested synthesis and secretion of sulfated glycoproteins in luminal epithelia of rat uteri. 1. 35SO4 administered intraperitoneally was specifically incorporated into a glycopeptide component separated by electrophoresis of the glycosaminoglycan fraction prepared from the uterine epithelia (luminal), as well as the uterine fluid of ovariectomized rats treated with estradiol-17 beta, in contrast to the rats without estrogen treatment. 2. The epithelial cells of uteri isolated from estrogen-treated ovariectomized rats incorporated 35SO4 in vitro into at least two macromolecular components. The larger molecular weight component (sodium dodecyl sulfate polyacryl-amide gel electrophoresis and/or gel filtration) labelled with 35S was observed in both the cytosol and particulate fractions, whereas the smaller molecular weight component labelled with 35S was found only in the particulate fraction. 35SO4 was also incorporated into two macromolecular components in the incubation medium, similarly to the particulate fraction. A 35 SO4-labelled glycopeptide similar to that from the epithelial particulate fraction and the incubation medium, and not from the epithelial cytosol fraction. 3. Progesterone, in contrast to estrogen, did not stimulate the sulfated blycoprotein synthesis. Moreover, progesterone administered together with or after estrogen-administration completely arrested the estrogen-dependent synthesis and secretion of the sulfated glycoproteins in the uterine epithelia."}
{"id": "PMID:476158", "title": "Properties and affinity purification of the mixed-type putative acetylcholine receptor from wild and a mutant strain of hose flies.", "content": "Binding of decamethonium to a soluble preparation from house fly head (either wild or a mutant strain) showed a single kind of binding with values for wild strain of Kd = 0.095 micrometers and Bmax = 0.22 nmol/mg protein. The mutant had a four-fold greater affinity and a seven-fold lesser amount. The binding was blocked by both nicotinic and muscarinic drugs. The decamethonium binding migrated in sucrose gradients as a single peak, with sedimentation coefficient s20,w = 12.5 S and therefore a molecular weight of 342 000. Purification by affinity chromatography was achieved with only partial loss of activity, andthe purified material demonstrated a single band on analytical disc gel electrophoresis. Electrophoresis in sodium dodecyl sulphate gels showed two subunits of molecular wegiths 94 000 and 64 000. Both subunits had an isoelectric point of 4.8.", "contents": "Properties and affinity purification of the mixed-type putative acetylcholine receptor from wild and a mutant strain of hose flies. Binding of decamethonium to a soluble preparation from house fly head (either wild or a mutant strain) showed a single kind of binding with values for wild strain of Kd = 0.095 micrometers and Bmax = 0.22 nmol/mg protein. The mutant had a four-fold greater affinity and a seven-fold lesser amount. The binding was blocked by both nicotinic and muscarinic drugs. The decamethonium binding migrated in sucrose gradients as a single peak, with sedimentation coefficient s20,w = 12.5 S and therefore a molecular weight of 342 000. Purification by affinity chromatography was achieved with only partial loss of activity, andthe purified material demonstrated a single band on analytical disc gel electrophoresis. Electrophoresis in sodium dodecyl sulphate gels showed two subunits of molecular wegiths 94 000 and 64 000. Both subunits had an isoelectric point of 4.8."}
{"id": "PMID:476159", "title": "Characterization of endogenous folate and incorporation of labelled folates into the brain of the South African fruit bat.", "content": "Folate in the brain of the South African fruit bat consists of 10-formyltetrahydropteroyglutamic acid and the tri-, tetra- and penta- forms of 5-methyltetrahydropteroylglutamic acid. Following parenteral injection, only 5-[3H]-methyltetrahydropteroylglutamic acid was taken up by the brain, but none of a dose of 14C-labelled tetrahydropteroylglutamic acid was detectable. Only trace smounts of the 5-methyltetrahydropteroylglutamic acid were converted into the formyl compounds and a small amount of methyltetrahydropteroyltriglutamic acid appeared after 96 h. There was no significant difference in vitamin B-12-deficient animals.", "contents": "Characterization of endogenous folate and incorporation of labelled folates into the brain of the South African fruit bat. Folate in the brain of the South African fruit bat consists of 10-formyltetrahydropteroyglutamic acid and the tri-, tetra- and penta- forms of 5-methyltetrahydropteroylglutamic acid. Following parenteral injection, only 5-[3H]-methyltetrahydropteroylglutamic acid was taken up by the brain, but none of a dose of 14C-labelled tetrahydropteroylglutamic acid was detectable. Only trace smounts of the 5-methyltetrahydropteroylglutamic acid were converted into the formyl compounds and a small amount of methyltetrahydropteroyltriglutamic acid appeared after 96 h. There was no significant difference in vitamin B-12-deficient animals."}
{"id": "PMID:476160", "title": "Ionic partial molar volumes of density gradient salts at finite concentrations.", "content": "A general method is presented for estimating concentration-dependent partial molar volumes for individual ions of heavy density gradient salts used in the isopycnic ultracentrifugation of charged macromolecules.", "contents": "Ionic partial molar volumes of density gradient salts at finite concentrations. A general method is presented for estimating concentration-dependent partial molar volumes for individual ions of heavy density gradient salts used in the isopycnic ultracentrifugation of charged macromolecules."}
{"id": "PMID:476161", "title": "The increase in hexokinase activity in hereditary avian muscular dystrophy.", "content": "Hexokinase activity was found to be increased in both the more severely affected red (thigh) muscle of dystrophic chickens. The increase in activity was largely associated with the particulate fraction. These findings may indicate early events in the pathogenesis of avian muscular dystrophy.", "contents": "The increase in hexokinase activity in hereditary avian muscular dystrophy. Hexokinase activity was found to be increased in both the more severely affected red (thigh) muscle of dystrophic chickens. The increase in activity was largely associated with the particulate fraction. These findings may indicate early events in the pathogenesis of avian muscular dystrophy."}
{"id": "PMID:476163", "title": "[Comparative study of the characteristics of the proton NMR spin echo decay of different globular proteins in water].", "content": "Spin-echo decays were studied for protons of 7 enzymes and 4 other globular proteins. Similar decay behaviour of the skin-echo decay curves was observed for all non-enzymatic proteins (motion of protein globules as whole units was taken into account). The spin-echo decay surves of 5 monomeric enzymes also exhibited very close decay patterns, although they decayed more slowly than non-enzymatic proteins. Greater slowing of the decay was observed for proton spin echoes in two subunit enzymes. Observed slowing of the decay is interpreted in terms of more intense inner motions within the macromolecules of enzymes, compared to non-enzymes. In subunit enzymes it occurs in addition because of the motion of separate units.", "contents": "[Comparative study of the characteristics of the proton NMR spin echo decay of different globular proteins in water]. Spin-echo decays were studied for protons of 7 enzymes and 4 other globular proteins. Similar decay behaviour of the skin-echo decay curves was observed for all non-enzymatic proteins (motion of protein globules as whole units was taken into account). The spin-echo decay surves of 5 monomeric enzymes also exhibited very close decay patterns, although they decayed more slowly than non-enzymatic proteins. Greater slowing of the decay was observed for proton spin echoes in two subunit enzymes. Observed slowing of the decay is interpreted in terms of more intense inner motions within the macromolecules of enzymes, compared to non-enzymes. In subunit enzymes it occurs in addition because of the motion of separate units."}
{"id": "PMID:476167", "title": "[Conformation of hydrocarbon chains in a lipid bilayer].", "content": "Based on the linear relationship between the entropy change deltaS during the phase transition and the length of he hydrocarbon chains of some lipids, the dependence of the probability of the trans--(pt) and gauche--(pg) conformation of the \"averaged\" methylene group of the chain on the increment deltas is deduced. Values of the probabilities for liquid n-alkanes, tri-glycerines and fatty acids are pt approximately equal to 0.6 and pg+ approximately equal to pg- approximately equal to 0.2, for liquid crystalline lecithin bilayers pt approximately equal to 0.8 and pg+ = pg- approximately equal to 0.1. From these data the average number of gaucheisomers per a plamitin chain in the dipalmitoylphosphatidylcholine molecule is estimated to be approximately 2.8-3.", "contents": "[Conformation of hydrocarbon chains in a lipid bilayer]. Based on the linear relationship between the entropy change deltaS during the phase transition and the length of he hydrocarbon chains of some lipids, the dependence of the probability of the trans--(pt) and gauche--(pg) conformation of the \"averaged\" methylene group of the chain on the increment deltas is deduced. Values of the probabilities for liquid n-alkanes, tri-glycerines and fatty acids are pt approximately equal to 0.6 and pg+ approximately equal to pg- approximately equal to 0.2, for liquid crystalline lecithin bilayers pt approximately equal to 0.8 and pg+ = pg- approximately equal to 0.1. From these data the average number of gaucheisomers per a plamitin chain in the dipalmitoylphosphatidylcholine molecule is estimated to be approximately 2.8-3."}
{"id": "PMID:476162", "title": "[Study of the kinetics of chlorophyll photosensitized dye reduction by photopolarography].", "content": "It has been shown by photopolarographic method that under stationary illumination chlorophyll-photosensitized reduction of dyes with ascorbic acid proceeds according to the first kinetic order. Rate constants of photoreduction of vitamin K3, methelene red, safranin-T and methylviologen were found in the course of sensitization which proceeded by oxidative and reduction mechanisms. Similarity of kinetics at both sensitization mechanisms was shown. Results obtained by polarographic and spectroscopic methods were compared.", "contents": "[Study of the kinetics of chlorophyll photosensitized dye reduction by photopolarography]. It has been shown by photopolarographic method that under stationary illumination chlorophyll-photosensitized reduction of dyes with ascorbic acid proceeds according to the first kinetic order. Rate constants of photoreduction of vitamin K3, methelene red, safranin-T and methylviologen were found in the course of sensitization which proceeded by oxidative and reduction mechanisms. Similarity of kinetics at both sensitization mechanisms was shown. Results obtained by polarographic and spectroscopic methods were compared."}
{"id": "PMID:476178", "title": "[Mechanisms of adaptive regulation of complex motor systems (regulation with respect to position and acceleration)].", "content": "Possible mechanisms of adaptive control over human and animal motor systems are described for the case when the information about position or movement acceleration of its parts is used. A model of the control system with the standard which functions in two modes of operations is treated. The first mode is distinguished by measured control signal leaps used for the identification of a system state and for a further movement correction and precise definition of active muscle composition. The second mode is characterized by the estimation of movement direction only, in case when a certain value of a regulated parameter is maintained or reached. Some problems of the control over a multilinked system are also discussed.", "contents": "[Mechanisms of adaptive regulation of complex motor systems (regulation with respect to position and acceleration)]. Possible mechanisms of adaptive control over human and animal motor systems are described for the case when the information about position or movement acceleration of its parts is used. A model of the control system with the standard which functions in two modes of operations is treated. The first mode is distinguished by measured control signal leaps used for the identification of a system state and for a further movement correction and precise definition of active muscle composition. The second mode is characterized by the estimation of movement direction only, in case when a certain value of a regulated parameter is maintained or reached. Some problems of the control over a multilinked system are also discussed."}
{"id": "PMID:476187", "title": "The effects of EMG-assisted relaxation training with hyperkinetic children: a behavioral alternative.", "content": "Six developmentally hyperkinetic children, ages 6 to 9, participated in either an A-B-A-B-A or an A-B-A-BC-A research design to evaluate the effect of EMG-assisted relaxation training in the acquisition of self regulatory skills to decrease hyperkinetic behavior. Descriptive verbal feedback regarding subject performance was provided during the BC phase in addition to the continuous rate-proportional auditory feedback normally available. Posttraining psychological assessment yielded significant gains for the experimental group on various visual motor tasks at the .05 level. All six subjects significantly decreased EMG activity across the 15 sessions. In a follow-up, five of the six maintained such activity at or below levels reached at the conclusion of the study. Five out of six made significant behavioral improvements at home during training and were maintaining these at the follow up. One out of four made significant improvements in school behavior during training, with five out of six exhibiting such improvements at the follow-up during the next school year. Auditory feedback was effective at the .05 level; the benefit of the verbal feedback, however, was not demonstrated. Two subjects were maintained on stimulant medication, one with a slight dose reduction during training. According to the observations of their parents and teachers, four subjects for whom medication had been recommended prior to the training were subsequently judged not to need it at the time of the follow-up.", "contents": "The effects of EMG-assisted relaxation training with hyperkinetic children: a behavioral alternative. Six developmentally hyperkinetic children, ages 6 to 9, participated in either an A-B-A-B-A or an A-B-A-BC-A research design to evaluate the effect of EMG-assisted relaxation training in the acquisition of self regulatory skills to decrease hyperkinetic behavior. Descriptive verbal feedback regarding subject performance was provided during the BC phase in addition to the continuous rate-proportional auditory feedback normally available. Posttraining psychological assessment yielded significant gains for the experimental group on various visual motor tasks at the .05 level. All six subjects significantly decreased EMG activity across the 15 sessions. In a follow-up, five of the six maintained such activity at or below levels reached at the conclusion of the study. Five out of six made significant behavioral improvements at home during training and were maintaining these at the follow up. One out of four made significant improvements in school behavior during training, with five out of six exhibiting such improvements at the follow-up during the next school year. Auditory feedback was effective at the .05 level; the benefit of the verbal feedback, however, was not demonstrated. Two subjects were maintained on stimulant medication, one with a slight dose reduction during training. According to the observations of their parents and teachers, four subjects for whom medication had been recommended prior to the training were subsequently judged not to need it at the time of the follow-up."}
{"id": "PMID:476188", "title": "Alpha detection: some comments on Hardt and Kamiya, \"Conflicting results in EEG alpha feedback studies\".", "content": "The problem of defining EEG alpha activity, and of detecting and measuring it, is outlined. Some advantages of a threshold method are presented and compared with an amplitude-integration method. Characteristics of percent-time scoring, based upon observable alpha spindles in the waking EEG, are discussed. A probability distribution of percent-time alpha as a function of detection threshold is derived and compared with empirical data.", "contents": "Alpha detection: some comments on Hardt and Kamiya, \"Conflicting results in EEG alpha feedback studies\". The problem of defining EEG alpha activity, and of detecting and measuring it, is outlined. Some advantages of a threshold method are presented and compared with an amplitude-integration method. Characteristics of percent-time scoring, based upon observable alpha spindles in the waking EEG, are discussed. A probability distribution of percent-time alpha as a function of detection threshold is derived and compared with empirical data."}
{"id": "PMID:476189", "title": "Biofeedback control of skin potential level.", "content": "This study explored the possibility of using biofeedback to arrest spontaneous declines in tonic electrodermal levels. For 3 days, 10 subjects (S) received 20 minutes of training in increasing skin potential level (SPL) negativity (arrested declines), followed by 20 minutes of training in decreasing SPL negativity (facilitating declines), with reverse order for half of the Ss. Simultaneous analogue and binary feedback of SPL were used. Training in the direction of SPL increases resulted in arrest of spontaneous declines in SPL negativity but did not result in large-magnitude increases above baseline. Training in the direction of SPL decreases facilitated declines in SPL. The absolute mean difference between the increase condition and the decrease condition on the 3rd day was 8.28 mV. Correlations suggested that considerable intraindividual variability may have been independently related to the Locus of Control personality dimension and to a Law of Initial Values limitation on SPL change. It was concluded that SPL was controllable to the extent of facilitating or arresting spontaneous declines.", "contents": "Biofeedback control of skin potential level. This study explored the possibility of using biofeedback to arrest spontaneous declines in tonic electrodermal levels. For 3 days, 10 subjects (S) received 20 minutes of training in increasing skin potential level (SPL) negativity (arrested declines), followed by 20 minutes of training in decreasing SPL negativity (facilitating declines), with reverse order for half of the Ss. Simultaneous analogue and binary feedback of SPL were used. Training in the direction of SPL increases resulted in arrest of spontaneous declines in SPL negativity but did not result in large-magnitude increases above baseline. Training in the direction of SPL decreases facilitated declines in SPL. The absolute mean difference between the increase condition and the decrease condition on the 3rd day was 8.28 mV. Correlations suggested that considerable intraindividual variability may have been independently related to the Locus of Control personality dimension and to a Law of Initial Values limitation on SPL change. It was concluded that SPL was controllable to the extent of facilitating or arresting spontaneous declines."}
{"id": "PMID:476166", "title": "[Conformational transitions of DNA in concentrated neutral salt solutions].", "content": "Salt anions can be arranged in lyotropic series of the action of conformational stability of DNA. This effect is universal for proteins and DNA. It is explained by the salt changes of transfer free energy of macromolecular inner groups to the solvent. Effect mechanism is the combination of anion direct interaction with the exposed inner groups and in indirect way through the changes of water structure. Individual features of the salt effect on DNA are the induced transitions in DNA duplex and the changes of differential stability of AT- and GC-base pairs. An important role in these phenomena plays the dehydration of DNA in the reduction of water activity in concentrated salt solutions. Local changes in medium polarity, hydration level of DNA molecule at different stages of their functioning can explain the regulatory role in intracellular processes, as well as selectivity and specificity of the action of individual ions in the cell.", "contents": "[Conformational transitions of DNA in concentrated neutral salt solutions]. Salt anions can be arranged in lyotropic series of the action of conformational stability of DNA. This effect is universal for proteins and DNA. It is explained by the salt changes of transfer free energy of macromolecular inner groups to the solvent. Effect mechanism is the combination of anion direct interaction with the exposed inner groups and in indirect way through the changes of water structure. Individual features of the salt effect on DNA are the induced transitions in DNA duplex and the changes of differential stability of AT- and GC-base pairs. An important role in these phenomena plays the dehydration of DNA in the reduction of water activity in concentrated salt solutions. Local changes in medium polarity, hydration level of DNA molecule at different stages of their functioning can explain the regulatory role in intracellular processes, as well as selectivity and specificity of the action of individual ions in the cell."}
{"id": "PMID:476185", "title": "[Successive phases in continuous work of human skeletal muscle during dentate tetanus functioning].", "content": "The sustained unfused tetanus of the human flexor digitorum sublimis was studied in isometrical conditions during electrical stimulation of the muscle. The frequency of stimulation was 8-12 p.p.s., train duration 20-60 s. 3 distinct successive phases of tetanus were revealed. These phases differ in the ratio of the mean tension to the amplitude of its oscillations, in relaxation rate and the efficiency of the duble pulse action. The duration of phases depends on muscle length, stimulation frequency and previous activity. It is supposed that phase II is adopted for the quick movements and muscle properties in phase III for the efficient mantaining of sustained tension.", "contents": "[Successive phases in continuous work of human skeletal muscle during dentate tetanus functioning]. The sustained unfused tetanus of the human flexor digitorum sublimis was studied in isometrical conditions during electrical stimulation of the muscle. The frequency of stimulation was 8-12 p.p.s., train duration 20-60 s. 3 distinct successive phases of tetanus were revealed. These phases differ in the ratio of the mean tension to the amplitude of its oscillations, in relaxation rate and the efficiency of the duble pulse action. The duration of phases depends on muscle length, stimulation frequency and previous activity. It is supposed that phase II is adopted for the quick movements and muscle properties in phase III for the efficient mantaining of sustained tension."}
{"id": "PMID:476184", "title": "[Dynamics of intramembrane particles during proteolysis of certain membranes].", "content": "Freeze-fracture electron microscopy has been used to study the ultrastructure of proteolytic enzymes treated of the bovine photoreceptor membranes, the rat liver microsome ghosts and the rabbit sarcoplasmic reticulum membranes. The observed increase in the intramembranous particle number in the inner fracture face suggests transmembrane dipping of amphipathic integral proteins affected by the partial proteolysis.", "contents": "[Dynamics of intramembrane particles during proteolysis of certain membranes]. Freeze-fracture electron microscopy has been used to study the ultrastructure of proteolytic enzymes treated of the bovine photoreceptor membranes, the rat liver microsome ghosts and the rabbit sarcoplasmic reticulum membranes. The observed increase in the intramembranous particle number in the inner fracture face suggests transmembrane dipping of amphipathic integral proteins affected by the partial proteolysis."}
{"id": "PMID:476174", "title": "[Ionic balance during cultivation of cell populations in a spatially homogeneous medium].", "content": "In culture medium many ionic reactions proceed. Extracellular substances and the cell proper participate in these reactions. For the analysis of ionic processes proceeding in this system the classical approach of physical chemistry is broadened, the effect of flow and cells being taken into account. Fast and slow variables are divided. Ionic equilibria in culture medium are subjected to the same laws as in the absence of the flow and cells. Slow variables are common concentrations of substances of each type. An equation for the cell charge is derived. The set of equations derived describes the dependence of pH culture medium, concentration of the biomass, substrates, metabolic products, charge of these substances on flow rates and metabolism stechiometry.", "contents": "[Ionic balance during cultivation of cell populations in a spatially homogeneous medium]. In culture medium many ionic reactions proceed. Extracellular substances and the cell proper participate in these reactions. For the analysis of ionic processes proceeding in this system the classical approach of physical chemistry is broadened, the effect of flow and cells being taken into account. Fast and slow variables are divided. Ionic equilibria in culture medium are subjected to the same laws as in the absence of the flow and cells. Slow variables are common concentrations of substances of each type. An equation for the cell charge is derived. The set of equations derived describes the dependence of pH culture medium, concentration of the biomass, substrates, metabolic products, charge of these substances on flow rates and metabolism stechiometry."}
{"id": "PMID:476180", "title": "[Photosensitized luminescence of singlet oxygen in aqueous solutions].", "content": "The photoluminescence of singlet oxygen has been observed in air saturated solutions of riboflavin in D2O and mixtures of D2O and H2O. The excitation spectrum coincides with the absorption spectrum of the pigment, the emission maximum lies at 1275 nm. In D2O the quantum yield is approximately 1,2 x 10(-7). H2O quenches the luminescence. Analysis of quenching has shown that the quantum yield in H2O is less than in D2O by the factor of 20.", "contents": "[Photosensitized luminescence of singlet oxygen in aqueous solutions]. The photoluminescence of singlet oxygen has been observed in air saturated solutions of riboflavin in D2O and mixtures of D2O and H2O. The excitation spectrum coincides with the absorption spectrum of the pigment, the emission maximum lies at 1275 nm. In D2O the quantum yield is approximately 1,2 x 10(-7). H2O quenches the luminescence. Analysis of quenching has shown that the quantum yield in H2O is less than in D2O by the factor of 20."}
{"id": "PMID:476190", "title": "Operant conditioning of brain steady potential shifts in man.", "content": "Steady potential shifts (SPS) recorded from the scalp were conditioned operantly by visual and acoustical feedback. Three groups of seven subjects were each tested with a different response-reinforcement contingency: positive reinforcement for a positive SPS after a cue stimulus, positive reinforcement for a negative SPS after a cue stimulus, and noncontingent reinforcement. The steady potential shifts learned under these three conditions differed significantly. Negative shifts were associated with subjective feelings of activation, positive shifts with inactivation. Cortical genesis and possible artifacts are discussed.", "contents": "Operant conditioning of brain steady potential shifts in man. Steady potential shifts (SPS) recorded from the scalp were conditioned operantly by visual and acoustical feedback. Three groups of seven subjects were each tested with a different response-reinforcement contingency: positive reinforcement for a positive SPS after a cue stimulus, positive reinforcement for a negative SPS after a cue stimulus, and noncontingent reinforcement. The steady potential shifts learned under these three conditions differed significantly. Negative shifts were associated with subjective feelings of activation, positive shifts with inactivation. Cortical genesis and possible artifacts are discussed."}
{"id": "PMID:476191", "title": "Biofeedback as therapy in Raynaud's disease.", "content": "The following dimensions of Raynaud's disease are reviewed: (a) etiological factors, particularly those of a psychological nature, (b) proposed biological mechanisms of vasospastic episodes, (c) efficacy of pharmaceutical and surgical interventions, and (d) use of biofeedback as therapy. Emotional stress appears to be wholly sufficient to induce vasopastic episodes in victims. Some authors further hypothesize that suppressed anger may be involved in the phenomenon. Vehicles proposed to account for the symptoms include local vascular pathology, sympathetic discharge, and biochemical phenomena. Sympathectomy does not produce permanent remission and vasodilator medications tend to have undesirable side effects even when successful. Documented success with feedback techniques is sparse but encouraging. Published outcome studies are often confounded by the use of additional treatment modes such as relaxation training and hypnosis. The study of Raynaud's disease has enormous potential for the understanding of psychosomatic illness in a holistic fashion. Stresses induce immediate physiological responses, and corrective conditioning is easily implemented and monitored.", "contents": "Biofeedback as therapy in Raynaud's disease. The following dimensions of Raynaud's disease are reviewed: (a) etiological factors, particularly those of a psychological nature, (b) proposed biological mechanisms of vasospastic episodes, (c) efficacy of pharmaceutical and surgical interventions, and (d) use of biofeedback as therapy. Emotional stress appears to be wholly sufficient to induce vasopastic episodes in victims. Some authors further hypothesize that suppressed anger may be involved in the phenomenon. Vehicles proposed to account for the symptoms include local vascular pathology, sympathetic discharge, and biochemical phenomena. Sympathectomy does not produce permanent remission and vasodilator medications tend to have undesirable side effects even when successful. Documented success with feedback techniques is sparse but encouraging. Published outcome studies are often confounded by the use of additional treatment modes such as relaxation training and hypnosis. The study of Raynaud's disease has enormous potential for the understanding of psychosomatic illness in a holistic fashion. Stresses induce immediate physiological responses, and corrective conditioning is easily implemented and monitored."}
{"id": "PMID:476192", "title": "Treatment of hypertonicity in muscles of lip retraction.", "content": "An EMG biofeedback program was developed for a 56-year-old Parkinsonism patient who exhibited pathological lip hypertonia and retraction. The program was designed to achieve the following goals: (1) to demonstrate a reduction in postural lip hypertonicity and (2) to demonstrate a reduction in lip hypertonicity during a series of increasingly complex speech activities. To achieve the first goal, contrastive tasks of full contraction and relaxation were utilized. Each posture was sustained while voltage measurements were made at specific intervals. Procedures to modify lip retraction during speech included five tasks in which the patient was to monitor the audio feedback signal. The tasks involved: prolongation of a neutral vowel, consonant-vowel combinations, monosyllabic words, sentences, and a paragraph-reading task. Data collected over six biofeedback sessions are presented. Trend analyses showed consistent muscular reduction within each task. The following explanations for the decrease in the patient's hypertonicity were discussed: (1) reduction of anisometric contraction, (2) reduction of isometric contraction, (3) relearning of agonistic-antagonistic muscle balance.", "contents": "Treatment of hypertonicity in muscles of lip retraction. An EMG biofeedback program was developed for a 56-year-old Parkinsonism patient who exhibited pathological lip hypertonia and retraction. The program was designed to achieve the following goals: (1) to demonstrate a reduction in postural lip hypertonicity and (2) to demonstrate a reduction in lip hypertonicity during a series of increasingly complex speech activities. To achieve the first goal, contrastive tasks of full contraction and relaxation were utilized. Each posture was sustained while voltage measurements were made at specific intervals. Procedures to modify lip retraction during speech included five tasks in which the patient was to monitor the audio feedback signal. The tasks involved: prolongation of a neutral vowel, consonant-vowel combinations, monosyllabic words, sentences, and a paragraph-reading task. Data collected over six biofeedback sessions are presented. Trend analyses showed consistent muscular reduction within each task. The following explanations for the decrease in the patient's hypertonicity were discussed: (1) reduction of anisometric contraction, (2) reduction of isometric contraction, (3) relearning of agonistic-antagonistic muscle balance."}
{"id": "PMID:476165", "title": "[Study of structural transformations of the sugar-phosphate chain and nitrogen bases of DNA hydrate by IR-spectroscopy].", "content": "The IR-spectra (900-1800 cm-1) of films of native and denatured DNA were studied within the temperature range 4-100 degrees C at different relative humidity (r.h.). Two temperature intervals of S-like change of spectral parameters of absorption bands of sugar-phosphate chain of double-helical DNA are found on temperature relationships at high humidity. These two intervals point to two types of cooperative transitions of sugar-phosphate pattern. The band parameters of nitrous bases are changed only under high temperature transition. In the region of structural transitions of different subsystems of DNA hydrate hyperchromism was wound for the bands of sugar-phosphate chain and hydrate cover unlike hyperchromism specific of most nitrous bases bands. From the data obtained it is proposed to use the basic characteristics of temperature relationship of spectral parameters of the absorption bands of sugar-phosphate pattern and hydrate envelope in the region of low-temperature transition for the \"in vivo\" analysis of the DNA structure in complex biological systems.", "contents": "[Study of structural transformations of the sugar-phosphate chain and nitrogen bases of DNA hydrate by IR-spectroscopy]. The IR-spectra (900-1800 cm-1) of films of native and denatured DNA were studied within the temperature range 4-100 degrees C at different relative humidity (r.h.). Two temperature intervals of S-like change of spectral parameters of absorption bands of sugar-phosphate chain of double-helical DNA are found on temperature relationships at high humidity. These two intervals point to two types of cooperative transitions of sugar-phosphate pattern. The band parameters of nitrous bases are changed only under high temperature transition. In the region of structural transitions of different subsystems of DNA hydrate hyperchromism was wound for the bands of sugar-phosphate chain and hydrate cover unlike hyperchromism specific of most nitrous bases bands. From the data obtained it is proposed to use the basic characteristics of temperature relationship of spectral parameters of the absorption bands of sugar-phosphate pattern and hydrate envelope in the region of low-temperature transition for the \"in vivo\" analysis of the DNA structure in complex biological systems."}
{"id": "PMID:476182", "title": "[Heavy cation position in the structure of the crystalline B-form of DNA].", "content": "Coordinates of caesium ions in DNA crystalline B-form were obtained by X-ray study of CsDNA with the use of synchrotron radiation. In conformity with the model that well meets the obtained set of structure factor amplitudes, the cations are located rather close to diad axes lying between the planes of successive nucleotide pairs. The cations in the narrow groove touch DNA surface, while those in the wide groove are rather far from the DNA molecule.", "contents": "[Heavy cation position in the structure of the crystalline B-form of DNA]. Coordinates of caesium ions in DNA crystalline B-form were obtained by X-ray study of CsDNA with the use of synchrotron radiation. In conformity with the model that well meets the obtained set of structure factor amplitudes, the cations are located rather close to diad axes lying between the planes of successive nucleotide pairs. The cations in the narrow groove touch DNA surface, while those in the wide groove are rather far from the DNA molecule."}
{"id": "PMID:476186", "title": "[Structure of the B-form of DNA using the homopolymer poly(dA) . poly(dT) as an example. Refinement in accordance with x-ray and energy criteria].", "content": "The refinement of the B-form DNA structure was made using homopolymer poly(dA) (dT) as a model system. In the course of refinement 9 conformational and 22 independent experimental parameters (20 intensities of reflections and 2 helical parameters) were used. After the goal-function, including R-factor, helical and conformational parameters, was optimised, the structures with the values of potential energy and R-factor close correspondingly to the optimal values were selected. \"The best\" conformations obtained according to energy criteria have the value of an axial projection per one nucleotide pair of about 2,9 A that is equal to the corresponding value in the recently discovered relaxed form of DNA in solution. The most probable conformations according to combine energy and structural criteria have better value of the R-factor in comparison with those derived on the basis of X-ray only, although they are slightly less satisfactory according to the energy criteria. The conformation of DNA which have the values of an axial projection per one nucleotide pair--3,29 A; of the angle of rotation of neighboring nucleotide pair relative each other--36 degrees and of the angle of nucleotide pair with an axis of the helix -13,7 degrees seems to be the most accurate description of the doublehelical structure of poly(dA) . poly(dT). The atomic coordinates of the new model may be used in analysis of stereochemistry of the complexes of nucleic acids with polypeptide and proteins, that is in consideration of the problem of protein-nucleic acid recognition.", "contents": "[Structure of the B-form of DNA using the homopolymer poly(dA) . poly(dT) as an example. Refinement in accordance with x-ray and energy criteria]. The refinement of the B-form DNA structure was made using homopolymer poly(dA) (dT) as a model system. In the course of refinement 9 conformational and 22 independent experimental parameters (20 intensities of reflections and 2 helical parameters) were used. After the goal-function, including R-factor, helical and conformational parameters, was optimised, the structures with the values of potential energy and R-factor close correspondingly to the optimal values were selected. \"The best\" conformations obtained according to energy criteria have the value of an axial projection per one nucleotide pair of about 2,9 A that is equal to the corresponding value in the recently discovered relaxed form of DNA in solution. The most probable conformations according to combine energy and structural criteria have better value of the R-factor in comparison with those derived on the basis of X-ray only, although they are slightly less satisfactory according to the energy criteria. The conformation of DNA which have the values of an axial projection per one nucleotide pair--3,29 A; of the angle of rotation of neighboring nucleotide pair relative each other--36 degrees and of the angle of nucleotide pair with an axis of the helix -13,7 degrees seems to be the most accurate description of the doublehelical structure of poly(dA) . poly(dT). The atomic coordinates of the new model may be used in analysis of stereochemistry of the complexes of nucleic acids with polypeptide and proteins, that is in consideration of the problem of protein-nucleic acid recognition."}
{"id": "PMID:476193", "title": "The use of temperature biofeedback in the treatment of chronic pain due to causalgia.", "content": "A patient with chronic pain due to a reflex sympathetic dystrophy in his hand and arm was successfully treated with temperature biofeedback after several months of conservative standard medical care brought little relief. Over the 18 treatment sessions the patient learned to emit a reliable handwarming response of 1 to 1.5 degrees C. Coincident with his learning, the pain in his hand and arm decreased markedly and remained absent at 1-year follow-up.", "contents": "The use of temperature biofeedback in the treatment of chronic pain due to causalgia. A patient with chronic pain due to a reflex sympathetic dystrophy in his hand and arm was successfully treated with temperature biofeedback after several months of conservative standard medical care brought little relief. Over the 18 treatment sessions the patient learned to emit a reliable handwarming response of 1 to 1.5 degrees C. Coincident with his learning, the pain in his hand and arm decreased markedly and remained absent at 1-year follow-up."}
{"id": "PMID:476195", "title": "Glycosylation of mouse embryonic hemoglobins in cultured yolk sac erythroblasts.", "content": "Embryonic hemoglobins of yolk sac nucleated erythroblasts were glycosylated in vitro by (3H)-glucose and the rate of glycosylation was maximal within 24 h of incubation. Isoelectric focusing of the glycosylated products revealed that both EI and EII embryonic hemoglobins have glycosylated components, EIg, EIIg, with pI very close to the native hemoglobins.", "contents": "Glycosylation of mouse embryonic hemoglobins in cultured yolk sac erythroblasts. Embryonic hemoglobins of yolk sac nucleated erythroblasts were glycosylated in vitro by (3H)-glucose and the rate of glycosylation was maximal within 24 h of incubation. Isoelectric focusing of the glycosylated products revealed that both EI and EII embryonic hemoglobins have glycosylated components, EIg, EIIg, with pI very close to the native hemoglobins."}
{"id": "PMID:476196", "title": "Influence of progressive starvation upon brain and adrenal monoaminergic activity in developing rats of two different ages.", "content": "Experiments show the influence of progressive starvation upon the synthetic and metabolic activity of monoaminergic function in the brain and the adrenal gland of young rats of two different ages. Brain and adrenal monoamine oxidase (MAO) showed a tendency to decline with the prolongation of the starvation interval. After 60 h of starvation, MAO activity was irreversibly decreased, even with 24 h of feeding, in the two age groups. Cerebral catechol-O-methyltransferase (COMT) activity was very slightly affected in response to the starvation in the older group, but the younger group showed an increased level of enzyme activity, and refeeding after 60 h of starvation of the young rats produced further increases. 60 h of starvation produced an increase in COMT activity of the adrenal gland of the older rats whereas the younger group did not show any marked change. Adrenal phenylethanolamine-N-methyltransferase (PNMT) declined after 24 and 48 h of starvation in the older rats, but the younger rats showed progressive increases after similar intervals of starvation. After 60 h of starvation, PNMT in the adrenal gland of the old rats increased significantly when compared to the control value, but the younger rats did not show any important change. Adrenal stores of adrenaline rose progressively up to 60 h of starvation in the old rats whereas the younger group responded in a contrary manner. Adrenal noradrenaline followed a similar pattern of evolution in both groups up to 60 h of starvation (when the results are expressed per milligram of adrenal protein), and refeeding had very little influence on the effects of starvation. The effects of starvation upon adrenal and cerebral MAO activity were verified with two different substrates. The results provide evidence that the metabolism of monoamines by oxidative deamination can be markedly affected by starvation, and this can be irreversible even after 24 h of feeding of starved rats. COMT activity augments when MAO activity declines.", "contents": "Influence of progressive starvation upon brain and adrenal monoaminergic activity in developing rats of two different ages. Experiments show the influence of progressive starvation upon the synthetic and metabolic activity of monoaminergic function in the brain and the adrenal gland of young rats of two different ages. Brain and adrenal monoamine oxidase (MAO) showed a tendency to decline with the prolongation of the starvation interval. After 60 h of starvation, MAO activity was irreversibly decreased, even with 24 h of feeding, in the two age groups. Cerebral catechol-O-methyltransferase (COMT) activity was very slightly affected in response to the starvation in the older group, but the younger group showed an increased level of enzyme activity, and refeeding after 60 h of starvation of the young rats produced further increases. 60 h of starvation produced an increase in COMT activity of the adrenal gland of the older rats whereas the younger group did not show any marked change. Adrenal phenylethanolamine-N-methyltransferase (PNMT) declined after 24 and 48 h of starvation in the older rats, but the younger rats showed progressive increases after similar intervals of starvation. After 60 h of starvation, PNMT in the adrenal gland of the old rats increased significantly when compared to the control value, but the younger rats did not show any important change. Adrenal stores of adrenaline rose progressively up to 60 h of starvation in the old rats whereas the younger group responded in a contrary manner. Adrenal noradrenaline followed a similar pattern of evolution in both groups up to 60 h of starvation (when the results are expressed per milligram of adrenal protein), and refeeding had very little influence on the effects of starvation. The effects of starvation upon adrenal and cerebral MAO activity were verified with two different substrates. The results provide evidence that the metabolism of monoamines by oxidative deamination can be markedly affected by starvation, and this can be irreversible even after 24 h of feeding of starved rats. COMT activity augments when MAO activity declines."}
{"id": "PMID:476197", "title": "Monoamine oxidase inhibition and recovery in the mouse brain at various ages after birth.", "content": "Mice between the ages of 1 day postpartum and adulthood were exposed to the monoamine oxidase inhibitors tranylcypromine and pargyline. The mice were killed at seven different times following drug injection and assayed in vitro for monoamine oxidase activity in four brain regions. Inhibition and recovery of monoamine oxidase activity varied by age, and with the two inhibitors used. During the early maturational period, the potential for new monoamine oxidase synthesis was greater than normal maturational patterns of increase would suggest; and during later maturation either the proportions of various monoamine oxidase forms or their rates of synthesis are different.", "contents": "Monoamine oxidase inhibition and recovery in the mouse brain at various ages after birth. Mice between the ages of 1 day postpartum and adulthood were exposed to the monoamine oxidase inhibitors tranylcypromine and pargyline. The mice were killed at seven different times following drug injection and assayed in vitro for monoamine oxidase activity in four brain regions. Inhibition and recovery of monoamine oxidase activity varied by age, and with the two inhibitors used. During the early maturational period, the potential for new monoamine oxidase synthesis was greater than normal maturational patterns of increase would suggest; and during later maturation either the proportions of various monoamine oxidase forms or their rates of synthesis are different."}
{"id": "PMID:476198", "title": "Electrokinetic behaviour of cord blood platelets in acidosis: an in vitro study.", "content": "The effect of acidosis on platelet surface potential was studied in vitro in samples obtained from newborn infants. It was found that acidosis induced by CO2 and lactic acid decreased the electrophoretic mobility of platelets in the presence of plasma. It is suggested that the changed electrokinetic properties of platelets in acidosis may be an additional factor contributing to bleeding manifestations and thrombotic complications often seen in babies with perinatal asphyxia.", "contents": "Electrokinetic behaviour of cord blood platelets in acidosis: an in vitro study. The effect of acidosis on platelet surface potential was studied in vitro in samples obtained from newborn infants. It was found that acidosis induced by CO2 and lactic acid decreased the electrophoretic mobility of platelets in the presence of plasma. It is suggested that the changed electrokinetic properties of platelets in acidosis may be an additional factor contributing to bleeding manifestations and thrombotic complications often seen in babies with perinatal asphyxia."}
{"id": "PMID:476199", "title": "Maternal and fetal plasma renin activity during ritodrine infusion to the mother.", "content": "The effect of ritodrine to the mother on plasma renin activity (PRA) in the mother and fetus was examined in 10 mothers coming to elective cesarean section. 10 comparable mothers without ritodrine infusion served as controls. At the end of 2 h infusion of ritodrine, the mean maternal PRA was significantly (p less than 0.001) higher than in the control group. Also the mean PRA levels in the umbilical vein (p less than 0.001) and umbilical artery (p less than 0.01) were significantly higher after ridodrine infusion to the mother than the respective levels in the control group.", "contents": "Maternal and fetal plasma renin activity during ritodrine infusion to the mother. The effect of ritodrine to the mother on plasma renin activity (PRA) in the mother and fetus was examined in 10 mothers coming to elective cesarean section. 10 comparable mothers without ritodrine infusion served as controls. At the end of 2 h infusion of ritodrine, the mean maternal PRA was significantly (p less than 0.001) higher than in the control group. Also the mean PRA levels in the umbilical vein (p less than 0.001) and umbilical artery (p less than 0.01) were significantly higher after ridodrine infusion to the mother than the respective levels in the control group."}
{"id": "PMID:476200", "title": "Correlations between neonatal body weight and adolescent brain development in rats.", "content": "Correlations between neonatal body weight, and body weight and several brain parameters at 30 days of age were studied in normal rats. At 30 days ('adolescence') cortex has already reached its final thickness and the rat exhibits long-term memory. Brain parameters included cerebral weight, DNA, protein and cholesterol contents and densities, as well as cortical and cerebral dimensions (cerebral sections). As expected, most of these parameters in 30-day-old animals were significantly correlated with each other. Unexpectedly, neonatal body weight was also significantly correlated with cholesterol content and density at 30 days, as well as with cortical and cerebral dimensions at 30 days. Thus, statistically, neonatal body weight already predetermines the extent of neuronal (cerebral) development at adolescence (30 days). This finding also makes it possible to make at birth statistical predictions about future brain development without having to sacrifice neonatal animals.", "contents": "Correlations between neonatal body weight and adolescent brain development in rats. Correlations between neonatal body weight, and body weight and several brain parameters at 30 days of age were studied in normal rats. At 30 days ('adolescence') cortex has already reached its final thickness and the rat exhibits long-term memory. Brain parameters included cerebral weight, DNA, protein and cholesterol contents and densities, as well as cortical and cerebral dimensions (cerebral sections). As expected, most of these parameters in 30-day-old animals were significantly correlated with each other. Unexpectedly, neonatal body weight was also significantly correlated with cholesterol content and density at 30 days, as well as with cortical and cerebral dimensions at 30 days. Thus, statistically, neonatal body weight already predetermines the extent of neuronal (cerebral) development at adolescence (30 days). This finding also makes it possible to make at birth statistical predictions about future brain development without having to sacrifice neonatal animals."}
{"id": "PMID:476170", "title": "[Spectral forms of chlorophyll and chloroplast structure of Chlamydomonas mutants with disortions in their light-gathering pigments].", "content": "A 2-3-fold reduction of chlorophyll content in pigment mutants of Chlamydomonas reinhardii is conditioned by the disturbances in light-harvesting pigments. When the activity of both photosystems is preserved, the reduction of short-wave forms of chlorophyll a (667, 678 nm) is accompanied by a fall of the fluorescence maximum at 685-690 nm; the disturbance of the long-wave forms with the adsorption maxima at 689, 698 an 703 nm results in a decrease of the chlorophyll fluorescence band at 707-718 nm. The long-wave forms of chlorophyll appertain to the pigment-protein complex of PS-1 which is not involved in the formation of contacts between the grand thylacoids.", "contents": "[Spectral forms of chlorophyll and chloroplast structure of Chlamydomonas mutants with disortions in their light-gathering pigments]. A 2-3-fold reduction of chlorophyll content in pigment mutants of Chlamydomonas reinhardii is conditioned by the disturbances in light-harvesting pigments. When the activity of both photosystems is preserved, the reduction of short-wave forms of chlorophyll a (667, 678 nm) is accompanied by a fall of the fluorescence maximum at 685-690 nm; the disturbance of the long-wave forms with the adsorption maxima at 689, 698 an 703 nm results in a decrease of the chlorophyll fluorescence band at 707-718 nm. The long-wave forms of chlorophyll appertain to the pigment-protein complex of PS-1 which is not involved in the formation of contacts between the grand thylacoids."}
{"id": "PMID:476203", "title": "Reincorporation of iodinated peptides in vivo and the comparative effectiveness of tungstic and trichloroacetic acids in peptide separation.", "content": "The absorption of 125I-bovine IgG after oral administration to young rats has been followed by gel filtration and quantitatively by TCA precipitation. The wide spectrum of protein derivatives crossing the gut wall have been utilised to compare the relative effectiveness of 10% TCA, and 1% tungstic acid, as reagents for the quantitative assessment of radioactive proteins and peptides. Radioactive iodide was the chief radioactive metabolite when 125I-labelled bovine IgG was fed to young rats, there was no significant incorporation of small radioactive metabolites into protein during a second exposure to the gut wall.", "contents": "Reincorporation of iodinated peptides in vivo and the comparative effectiveness of tungstic and trichloroacetic acids in peptide separation. The absorption of 125I-bovine IgG after oral administration to young rats has been followed by gel filtration and quantitatively by TCA precipitation. The wide spectrum of protein derivatives crossing the gut wall have been utilised to compare the relative effectiveness of 10% TCA, and 1% tungstic acid, as reagents for the quantitative assessment of radioactive proteins and peptides. Radioactive iodide was the chief radioactive metabolite when 125I-labelled bovine IgG was fed to young rats, there was no significant incorporation of small radioactive metabolites into protein during a second exposure to the gut wall."}
{"id": "PMID:476201", "title": "Maintenance of central and peripheral monoamine oxidase activity in developing rats subjected to disturbed alimentary rhythms and undernutrition.", "content": "Monoamine oxidase (MAO) activity was studied in whole brain, hypothalamus, adrenals and liver of developing rats subjected to disturbed feeding patterns or undernutrition for 3 weeks. The rats were divided into six groups: (1) normally fed controls; (2) rats starved for 24 h, fed for the following 8 h and killed after the last starvation period (PAS); (3) same treatment as in (2) but killed after the last feeding period (PAF); (4) rats starved for 16 h, fed for the following 8 h at a constant schedule, and killed after the last starvation period (PS); (5) same treatment as in (4) but killed after the last feeding period (PF), and (6) undernourished (U). Alteration of the feeding time resulted in significant decreases of MAO activity in the brain and the adrenal gland whereas the hypothalamus and the liver showed a slight increase in activity in the PAS group. In PS rats, MAO activity increased in the brain, adrenals and hypothalamus; in PF rats, the effects of the treatment were inverse. Both in the PS and PF rats, hepatic MAO activity was strongly decreased when assayed with kynuramine. In U rats, hepatic MAO activity was highly increased when assayed with kynuramine but the other tissues responded differently. The adrenaline and noradrenaline stocks of the adrenal gland were markedly increased in all the treated groups; the maximum increase in noradrenaline was observed in the PS rats. The results suggest that any disturbance in the feeding pattern affects the MAO activity in the central and peripheral regions of the young rat during postnatal development. The developing rat seems to get accustomed to new alimentary rhythms, and normal monoaminergic function is rapidly restored when the rat is given a compensatory diet. Increased adrenal catecholamines after a disturbance in the feeding patterns seem to be a response to stress.", "contents": "Maintenance of central and peripheral monoamine oxidase activity in developing rats subjected to disturbed alimentary rhythms and undernutrition. Monoamine oxidase (MAO) activity was studied in whole brain, hypothalamus, adrenals and liver of developing rats subjected to disturbed feeding patterns or undernutrition for 3 weeks. The rats were divided into six groups: (1) normally fed controls; (2) rats starved for 24 h, fed for the following 8 h and killed after the last starvation period (PAS); (3) same treatment as in (2) but killed after the last feeding period (PAF); (4) rats starved for 16 h, fed for the following 8 h at a constant schedule, and killed after the last starvation period (PS); (5) same treatment as in (4) but killed after the last feeding period (PF), and (6) undernourished (U). Alteration of the feeding time resulted in significant decreases of MAO activity in the brain and the adrenal gland whereas the hypothalamus and the liver showed a slight increase in activity in the PAS group. In PS rats, MAO activity increased in the brain, adrenals and hypothalamus; in PF rats, the effects of the treatment were inverse. Both in the PS and PF rats, hepatic MAO activity was strongly decreased when assayed with kynuramine. In U rats, hepatic MAO activity was highly increased when assayed with kynuramine but the other tissues responded differently. The adrenaline and noradrenaline stocks of the adrenal gland were markedly increased in all the treated groups; the maximum increase in noradrenaline was observed in the PS rats. The results suggest that any disturbance in the feeding pattern affects the MAO activity in the central and peripheral regions of the young rat during postnatal development. The developing rat seems to get accustomed to new alimentary rhythms, and normal monoaminergic function is rapidly restored when the rat is given a compensatory diet. Increased adrenal catecholamines after a disturbance in the feeding patterns seem to be a response to stress."}
{"id": "PMID:476205", "title": "Single injection clearance in the neonate.", "content": "The single injection clearance of inulin was compared to the standard clearance in neonates aged 1--20 days. In the first 3 days of life, the single injection method overestimated glomerular filtration rate by approximately 30%. From the 2nd week of life, values obtained by the two techniques were not significantly different. We conclude that caution should be paid when interpreting results obtained by the single injection technique during the very first days of life.", "contents": "Single injection clearance in the neonate. The single injection clearance of inulin was compared to the standard clearance in neonates aged 1--20 days. In the first 3 days of life, the single injection method overestimated glomerular filtration rate by approximately 30%. From the 2nd week of life, values obtained by the two techniques were not significantly different. We conclude that caution should be paid when interpreting results obtained by the single injection technique during the very first days of life."}
{"id": "PMID:476206", "title": "Immunochemical characterization of mature and immature glomerular basement membrane.", "content": "20 kidneys from premature infants (27th to 38th weeks of gestation), 5 kidneys of mature newborns and 5 kidneys of children between 5 and 15 years of age were obtained at necropsy and glomerular basement membranes (GBM) isolated. The isolated GBMs were degraded by papain and the degradation products were characterized by immunoelectrophoresis applying an antihuman GBM antiserum from the rabbit. GBMs of children between 5 and 15 years showed in each case 3 precipitation lines distributed from the alpha-gamma-zone. The examined newborns and 17 premature infants presented two precipitation lines only, moving with alpha 1-mobility and beta-gamma-inter-region. 3 premature infants showed a pattern with 2--4 different precipitation lines of different mobility, maybe interpretable as a result of bacterial digestion. On the grounds of these findings we postulate that the GBM is, from an immunochemical point of view, immature at birth and in the late fetal life becoming mature in the child with about 5.", "contents": "Immunochemical characterization of mature and immature glomerular basement membrane. 20 kidneys from premature infants (27th to 38th weeks of gestation), 5 kidneys of mature newborns and 5 kidneys of children between 5 and 15 years of age were obtained at necropsy and glomerular basement membranes (GBM) isolated. The isolated GBMs were degraded by papain and the degradation products were characterized by immunoelectrophoresis applying an antihuman GBM antiserum from the rabbit. GBMs of children between 5 and 15 years showed in each case 3 precipitation lines distributed from the alpha-gamma-zone. The examined newborns and 17 premature infants presented two precipitation lines only, moving with alpha 1-mobility and beta-gamma-inter-region. 3 premature infants showed a pattern with 2--4 different precipitation lines of different mobility, maybe interpretable as a result of bacterial digestion. On the grounds of these findings we postulate that the GBM is, from an immunochemical point of view, immature at birth and in the late fetal life becoming mature in the child with about 5."}
{"id": "PMID:476208", "title": "Assessment of ventilation-perfusion inequality by aADN2 in newborn infants.", "content": "The contribution (Qo/Qt) of gas-filled air spaces with reduced ventilation-perfusion ratio (VA/Qc) to the production of total venous admixture in nondistressed premature infant and newborn infants with transient tachypnea was assessed by the aADN2 and AaDo2. The mean value for Qo/Qt in both nondistressed prematures and infants with transient tachypnea was 0.08. In both groups this represented about 30% of total venous admixutre.", "contents": "Assessment of ventilation-perfusion inequality by aADN2 in newborn infants. The contribution (Qo/Qt) of gas-filled air spaces with reduced ventilation-perfusion ratio (VA/Qc) to the production of total venous admixture in nondistressed premature infant and newborn infants with transient tachypnea was assessed by the aADN2 and AaDo2. The mean value for Qo/Qt in both nondistressed prematures and infants with transient tachypnea was 0.08. In both groups this represented about 30% of total venous admixutre."}
{"id": "PMID:476207", "title": "Effects of low-dose prenatal corticosteroid administration on the premature rat.", "content": "Administration of dexamethasone (0.2 mg/kg/day) to the pregnant rat on days 19 and 20 of gestation has been found to have marked effects on the body and organ weight of fetuses delivered 1 day prematurely on day 21 of gestation. Longer-term dexamethasone treatment earlier in gestation (days 14-18) had even more pronounced effects on body and organ weights in rat fetuses delivered three days prematurely. Dexamethasone treatment for 2 days prior to delivery had no effect on the survival of 1-, 2- and 3-day premature rats. The marked inhibitory effect on organ and body weight observed in the premature rats resulted from prenatal steroid therapy approximating that used in human pregnancies (dexamethasone 0.2 mg/kg/day for 48 h prior to premature delivery).", "contents": "Effects of low-dose prenatal corticosteroid administration on the premature rat. Administration of dexamethasone (0.2 mg/kg/day) to the pregnant rat on days 19 and 20 of gestation has been found to have marked effects on the body and organ weight of fetuses delivered 1 day prematurely on day 21 of gestation. Longer-term dexamethasone treatment earlier in gestation (days 14-18) had even more pronounced effects on body and organ weights in rat fetuses delivered three days prematurely. Dexamethasone treatment for 2 days prior to delivery had no effect on the survival of 1-, 2- and 3-day premature rats. The marked inhibitory effect on organ and body weight observed in the premature rats resulted from prenatal steroid therapy approximating that used in human pregnancies (dexamethasone 0.2 mg/kg/day for 48 h prior to premature delivery)."}
{"id": "PMID:476209", "title": "Human fetal respiratory movements: a technique for noninvasive monitoring with the use of a tocodynamometer.", "content": "A tocodynamometer applied to the maternal abdomen is used for monitoring human fetal respiratory movements (FRM). This provides a recording of fetal chest wall movements from which observations and measurements relating to fetal respiratory physiology may be made. The FRM must be differentiated from materanal vascular and respiratory movements, as well as fetal movements and cardiac pulsations. The technique lends itself to extended periods of observation, since it does not transmit energy to the fetus as in other techniques. The simplicity of the technique, combined with the use of monitoring devices already available in most hospitals, warrants further observation of this method of respiratory movement monitoring in the developing fetus.", "contents": "Human fetal respiratory movements: a technique for noninvasive monitoring with the use of a tocodynamometer. A tocodynamometer applied to the maternal abdomen is used for monitoring human fetal respiratory movements (FRM). This provides a recording of fetal chest wall movements from which observations and measurements relating to fetal respiratory physiology may be made. The FRM must be differentiated from materanal vascular and respiratory movements, as well as fetal movements and cardiac pulsations. The technique lends itself to extended periods of observation, since it does not transmit energy to the fetus as in other techniques. The simplicity of the technique, combined with the use of monitoring devices already available in most hospitals, warrants further observation of this method of respiratory movement monitoring in the developing fetus."}
{"id": "PMID:476211", "title": "Neonatal changes in the plasma levels of cortisol, cortisone and aldosterone in the calf.", "content": "Plasma levels of cortisol, cortisone and aldosterone were measured during the first 20 days of life in 10 healthy calves. Cortisolemia, high at birth, decreased from birth to day 15. Changes in plasma cortisone were similar to those of cortisol. The value of the cortisol/cortisone plasma ratio increased from birth to day 8 and remained steady until day 20. A positive correlation was found between plasma levels of cortisol and cortisone, during the 6- to 20-day period; no correlation was observed before that. These results raise the question of whether, as is assumed in the human, maternal cortisol would cross the placenta with conversion to cortisone in the bovine. Plasma aldosterone increased from day 1 to day 3, and decreased steadily until day 20 when its level was very low. Negative correlation found between the values of aldosterone and sodium led us to think that natremia regulation by this hormone could be functional in the neonatal calf.", "contents": "Neonatal changes in the plasma levels of cortisol, cortisone and aldosterone in the calf. Plasma levels of cortisol, cortisone and aldosterone were measured during the first 20 days of life in 10 healthy calves. Cortisolemia, high at birth, decreased from birth to day 15. Changes in plasma cortisone were similar to those of cortisol. The value of the cortisol/cortisone plasma ratio increased from birth to day 8 and remained steady until day 20. A positive correlation was found between plasma levels of cortisol and cortisone, during the 6- to 20-day period; no correlation was observed before that. These results raise the question of whether, as is assumed in the human, maternal cortisol would cross the placenta with conversion to cortisone in the bovine. Plasma aldosterone increased from day 1 to day 3, and decreased steadily until day 20 when its level was very low. Negative correlation found between the values of aldosterone and sodium led us to think that natremia regulation by this hormone could be functional in the neonatal calf."}
{"id": "PMID:476210", "title": "Effect of development on the gangliosides of rabbit brain.", "content": "The effect of growth and development on the DNA and ganglioside-NANA of the rabbit forebrain, cerebellum and brainstem was studied. Animals were killed at 1 day of age and at intervals up to 180 days. The growth rate of each part of the brain fell during the first 30 days, but showed a spurt at about 40 days. The absolute amount of DNA reached its mature value by about 40 days. In the forebrain, the deposition of ganglioside-NANA rose sharply to a peak value at 50 days, falling to 120 days and rising again to 180 days, whereas in the cerebellum and brainstem the amount rose almost steadily to a mature value at 90 and 120 days, respectively. In the forebrain, the proportion of GD1a and GM1 showed a reciprocal rise and fall, respectively, during the first 10 days after birth, subsequently falling and rising to near mature values by 50 days. GD1a was the major ganglioside in the mature forebrain, whereas GT1 and GD1b were the major gangliosides in the mature cerebellum and in the mature brainstem, respectively.", "contents": "Effect of development on the gangliosides of rabbit brain. The effect of growth and development on the DNA and ganglioside-NANA of the rabbit forebrain, cerebellum and brainstem was studied. Animals were killed at 1 day of age and at intervals up to 180 days. The growth rate of each part of the brain fell during the first 30 days, but showed a spurt at about 40 days. The absolute amount of DNA reached its mature value by about 40 days. In the forebrain, the deposition of ganglioside-NANA rose sharply to a peak value at 50 days, falling to 120 days and rising again to 180 days, whereas in the cerebellum and brainstem the amount rose almost steadily to a mature value at 90 and 120 days, respectively. In the forebrain, the proportion of GD1a and GM1 showed a reciprocal rise and fall, respectively, during the first 10 days after birth, subsequently falling and rising to near mature values by 50 days. GD1a was the major ganglioside in the mature forebrain, whereas GT1 and GD1b were the major gangliosides in the mature cerebellum and in the mature brainstem, respectively."}
{"id": "PMID:476214", "title": "Plasma thyroid hormones and cholesterol in the newborn of genetically different types of cattle in a tropical environment.", "content": "The patterns of plasma concentrations of thyroxine (T4), triiodothyronine (T3) and cholesterol were determined in tropically adapted (ZX) and temperate (SH) breeds of cattle in the first 6 days of their lives in a tropical environment. T4 concentrations were high at birth and fell to within normal adult values at 6 days of age. In contrast, plasma cholesterol levels were low at birth and increased daily to the highest values on day 6. T3 levels increased from birth to maximum values at 12 h after birth and then declined progressively. The T4/T3 molar ratios were high at birth and decreased to lower values on day 2. T4 and cholesterol levels were  higher (p less than 0.001) in ZX than in SH calves, and in calves born in winter were higher (p less than 0.001) in females than in males. There were significant (p less than 0.001) animals within-breed differences in all parameters. The results are discussed with reference to the use of physiological and biochemical indices for the early selection of cattle suited to tropical conditions.", "contents": "Plasma thyroid hormones and cholesterol in the newborn of genetically different types of cattle in a tropical environment. The patterns of plasma concentrations of thyroxine (T4), triiodothyronine (T3) and cholesterol were determined in tropically adapted (ZX) and temperate (SH) breeds of cattle in the first 6 days of their lives in a tropical environment. T4 concentrations were high at birth and fell to within normal adult values at 6 days of age. In contrast, plasma cholesterol levels were low at birth and increased daily to the highest values on day 6. T3 levels increased from birth to maximum values at 12 h after birth and then declined progressively. The T4/T3 molar ratios were high at birth and decreased to lower values on day 2. T4 and cholesterol levels were  higher (p less than 0.001) in ZX than in SH calves, and in calves born in winter were higher (p less than 0.001) in females than in males. There were significant (p less than 0.001) animals within-breed differences in all parameters. The results are discussed with reference to the use of physiological and biochemical indices for the early selection of cattle suited to tropical conditions."}
{"id": "PMID:476213", "title": "Malonyldialdehyde formation, oxygen consumption, fatty acid composition in newborn platelets stimulated by thrombin.", "content": "The release reaction, the formation of malonyldialdehyde (MDA), the pattern of oxygen consumption, and the variation in fatty acid composition after addition of thrombin (1.67 U/ml) have been investigated in newborn platelets, comparing the obtained data with analogous values showed by adult platelets assumed as normal controls. Newborn platelets showed a release reaction 20% lower than that of controls. MDA formation, even in the presence of NEM and the burst in oxygen consumption, resulted to be similar in newborn and adult platelets (p greater than 0.3); the burst was also similar after the addition of thrombin (10 U/ml) in the presence of antimycin and aspirin. The ratio between formed MDA and oxygen consumption was 1:10 in adult platelets while it was 1:15 in those of newborns. The study of fatty acid composition demonstrated that in newborn platelets at rest, arachidonic acid is significantly in a higher concentration than in controls and that it decreases after stimulation with thrombin. It is concluded that the pathway of prostaglandins is normally stimulated by thrombin in newborn platelets.", "contents": "Malonyldialdehyde formation, oxygen consumption, fatty acid composition in newborn platelets stimulated by thrombin. The release reaction, the formation of malonyldialdehyde (MDA), the pattern of oxygen consumption, and the variation in fatty acid composition after addition of thrombin (1.67 U/ml) have been investigated in newborn platelets, comparing the obtained data with analogous values showed by adult platelets assumed as normal controls. Newborn platelets showed a release reaction 20% lower than that of controls. MDA formation, even in the presence of NEM and the burst in oxygen consumption, resulted to be similar in newborn and adult platelets (p greater than 0.3); the burst was also similar after the addition of thrombin (10 U/ml) in the presence of antimycin and aspirin. The ratio between formed MDA and oxygen consumption was 1:10 in adult platelets while it was 1:15 in those of newborns. The study of fatty acid composition demonstrated that in newborn platelets at rest, arachidonic acid is significantly in a higher concentration than in controls and that it decreases after stimulation with thrombin. It is concluded that the pathway of prostaglandins is normally stimulated by thrombin in newborn platelets."}
{"id": "PMID:476212", "title": "Organic acids in urine from preterm infants.", "content": "The organic acid excretion was studied in urine samples from 26 preterm infants on the 1st and 5th days of life and the results compared to those obtained in 5 samples from full-term neonates. Gas-chromatography-mass spectrometry with a computer system was the method used in this work. The acids tabulated were those more closely related to lactic acidosis and the Krebs cycle. Great variations were found in the excretion of these acids in preterm infants in contrast to the very homogeneous pattern obtained in full-term neonates.", "contents": "Organic acids in urine from preterm infants. The organic acid excretion was studied in urine samples from 26 preterm infants on the 1st and 5th days of life and the results compared to those obtained in 5 samples from full-term neonates. Gas-chromatography-mass spectrometry with a computer system was the method used in this work. The acids tabulated were those more closely related to lactic acidosis and the Krebs cycle. Great variations were found in the excretion of these acids in preterm infants in contrast to the very homogeneous pattern obtained in full-term neonates."}
{"id": "PMID:476215", "title": "Integrated cardiovascular responses to combined somatic and visceral afferent stimulation in newborn piglets.", "content": "Mean aortic pressure, arterial flow and heart rate response to interactions of afferent stimulation of the sciatic nerve (SNS) and carotid sinus stimulation by infusion (CSI) or inhibition by bilateral common carotid occlusion (BCCO) were studied in 25 newborn piglets under halothane-N2O anesthesia. Combined stimulation of the somatic and visceral afferents produced no heart rate changes. Combinations of pressor stimuli (BCCO and high frequency SNS) showed facilitation; femoral and renal resistances increased. However, combinations of depressor stimuli (CSI and low frequency SNS) showed occlusion. In combinations of pressor with depressor stimuli, the pressor pattern of responses dominated. Thus, dissimilar types of excitatory afferent inputs are capable of evoking facilitation in the neonatal cardiovascular regulatory system.", "contents": "Integrated cardiovascular responses to combined somatic and visceral afferent stimulation in newborn piglets. Mean aortic pressure, arterial flow and heart rate response to interactions of afferent stimulation of the sciatic nerve (SNS) and carotid sinus stimulation by infusion (CSI) or inhibition by bilateral common carotid occlusion (BCCO) were studied in 25 newborn piglets under halothane-N2O anesthesia. Combined stimulation of the somatic and visceral afferents produced no heart rate changes. Combinations of pressor stimuli (BCCO and high frequency SNS) showed facilitation; femoral and renal resistances increased. However, combinations of depressor stimuli (CSI and low frequency SNS) showed occlusion. In combinations of pressor with depressor stimuli, the pressor pattern of responses dominated. Thus, dissimilar types of excitatory afferent inputs are capable of evoking facilitation in the neonatal cardiovascular regulatory system."}
{"id": "PMID:476204", "title": "Light- and electron-microscopic studies on the gastric parietal cells in perinatal rats. Effects of fetal and neonatal oral administration of milk.", "content": "Perinatal changes of the gastric parietal cells were studied under normal and various experimental conditions. Histologic examination revealed that the parietal cells appeared markedly increased in number from late fetal to early neonatal days. When premature newborn rats were delivered by cesarean section and were nursed by foster mothers for 1 or 2 days, the degree of increase of these cells reached nearly that in the normal neonates. On the other hand, in fetuses retained in utero 1 day beyond the normal gestation, these cells appeared not increased in number. When these postmature fetuses were delivered, the parietal cells turned to increase in number in a degree similar to that in the normal neonates. Milk given to the premature newborn rats caused 6 h later a marked increase in the number of the parietal cells. Milk given to fetuses in utero also caused an increase of these cells. The results suggest that the development of the parietal cells in perinatal rats is accelerated by the intake of milk and that the fetal parietal cells are ready to respond to milk given at least 1 day before birth.", "contents": "Light- and electron-microscopic studies on the gastric parietal cells in perinatal rats. Effects of fetal and neonatal oral administration of milk. Perinatal changes of the gastric parietal cells were studied under normal and various experimental conditions. Histologic examination revealed that the parietal cells appeared markedly increased in number from late fetal to early neonatal days. When premature newborn rats were delivered by cesarean section and were nursed by foster mothers for 1 or 2 days, the degree of increase of these cells reached nearly that in the normal neonates. On the other hand, in fetuses retained in utero 1 day beyond the normal gestation, these cells appeared not increased in number. When these postmature fetuses were delivered, the parietal cells turned to increase in number in a degree similar to that in the normal neonates. Milk given to the premature newborn rats caused 6 h later a marked increase in the number of the parietal cells. Milk given to fetuses in utero also caused an increase of these cells. The results suggest that the development of the parietal cells in perinatal rats is accelerated by the intake of milk and that the fetal parietal cells are ready to respond to milk given at least 1 day before birth."}
{"id": "PMID:476216", "title": "Developmental pattern of pulmonary lipoprotein lipase in growing rats.", "content": "The developmental pattern of pulmonary lipoprotein lipase (LPL) activity was measured in growing male and female rats from the neonatal period to the adult age. The pulmonary phospholipid content showed a sharp increase at term from premature low level. At the same time the LPL activity increased markedly showing thereafter a decrease at 1 week of age and increasing thereafter to the adult age. Also the phospholipid content was low at 1 week of age. Thus the pulmonary phospholipid content and LPL activity change in the same way during the development indicating possibly that the LPL by supplying free fatty acids for the phospholipid biosynthesis may be one of the rate-limiting enzymes in the pulmonary surfactant synthesis.", "contents": "Developmental pattern of pulmonary lipoprotein lipase in growing rats. The developmental pattern of pulmonary lipoprotein lipase (LPL) activity was measured in growing male and female rats from the neonatal period to the adult age. The pulmonary phospholipid content showed a sharp increase at term from premature low level. At the same time the LPL activity increased markedly showing thereafter a decrease at 1 week of age and increasing thereafter to the adult age. Also the phospholipid content was low at 1 week of age. Thus the pulmonary phospholipid content and LPL activity change in the same way during the development indicating possibly that the LPL by supplying free fatty acids for the phospholipid biosynthesis may be one of the rate-limiting enzymes in the pulmonary surfactant synthesis."}
{"id": "PMID:476217", "title": "Pulmonary aeration after unilateral fetal ligation of pulmonary artery in neonatal rabbits.", "content": "When the pulmonary artery was ligated at the gestational age of 30 days, 9 out of the 17 rabbit fetuses had vital lungs and in 7 of these the lungs were aerated after breathing for 15 min to 1 h. Ligation before this gestational age caused necrotic lungs in all 13 rabbit fetuses. On the basis of this we can conclude that the bronchial circulation is not sufficient to ensure vitality of the fetal lung and the pulmonary circulation does not play a major role in the primary aeration of the lungs at birth.", "contents": "Pulmonary aeration after unilateral fetal ligation of pulmonary artery in neonatal rabbits. When the pulmonary artery was ligated at the gestational age of 30 days, 9 out of the 17 rabbit fetuses had vital lungs and in 7 of these the lungs were aerated after breathing for 15 min to 1 h. Ligation before this gestational age caused necrotic lungs in all 13 rabbit fetuses. On the basis of this we can conclude that the bronchial circulation is not sufficient to ensure vitality of the fetal lung and the pulmonary circulation does not play a major role in the primary aeration of the lungs at birth."}
{"id": "PMID:476218", "title": "Balance of net base in the rat. V. Effects of oral ammonium chloride loading.", "content": "The physiology of oral ammonium chloride loading was studied in four groups of male weanling rats weighing about 100 g and fed either standard ground Rostock rat food (containing 317 mmol net base/kg) or ground barley (containing only 20 mmol net base/kg). One group of animals on the Rostock diet received oral supplements of ammonium chloride (approximately 32 mmol . kg-1 . day-1) sufficient to provide a net zero rate of oral net base intake. In this group, ongoing fecal net base excretion caused net acid to be absorbed at an average rate of 15 mmol . kg-1 . day-1. The mean rate of renal net acid excretion rose markedly (by 29.4 mmol . kg-1 . day-1); and over an 8-day balance period the animals were able to maintain near-normal balances of net base. During a subsequent 8-day recovery the pattern of mineral turnover returned to normal. Even in the barley-fed rats, gastrointestinal net acid absorption was observed. These animals suffered a 61% reduction in the rate of body growth, but the overall rate of net base retention, per kilogram of mass gain, was close to the reference value. Finally, the combination of barley and ammonium chloride led to weight loss, positive net acid balances (8.5 mmol . kg-1 . day-1), and a maximal rate of renal net acid excretion (50.8 mmol . kg-1 . day-1). Some implications for the metabolism of organic acids are discussed.", "contents": "Balance of net base in the rat. V. Effects of oral ammonium chloride loading. The physiology of oral ammonium chloride loading was studied in four groups of male weanling rats weighing about 100 g and fed either standard ground Rostock rat food (containing 317 mmol net base/kg) or ground barley (containing only 20 mmol net base/kg). One group of animals on the Rostock diet received oral supplements of ammonium chloride (approximately 32 mmol . kg-1 . day-1) sufficient to provide a net zero rate of oral net base intake. In this group, ongoing fecal net base excretion caused net acid to be absorbed at an average rate of 15 mmol . kg-1 . day-1. The mean rate of renal net acid excretion rose markedly (by 29.4 mmol . kg-1 . day-1); and over an 8-day balance period the animals were able to maintain near-normal balances of net base. During a subsequent 8-day recovery the pattern of mineral turnover returned to normal. Even in the barley-fed rats, gastrointestinal net acid absorption was observed. These animals suffered a 61% reduction in the rate of body growth, but the overall rate of net base retention, per kilogram of mass gain, was close to the reference value. Finally, the combination of barley and ammonium chloride led to weight loss, positive net acid balances (8.5 mmol . kg-1 . day-1), and a maximal rate of renal net acid excretion (50.8 mmol . kg-1 . day-1). Some implications for the metabolism of organic acids are discussed."}
{"id": "PMID:476202", "title": "Effect of nicotine on the development of fetal and suckling rats.", "content": "Nicotine, administered at a dose of 100 micrograms/kg/day from day 14 of gestation, did not affect maternal food intake, weight gain, length of gestation, litter size or fetal development; however, a daily dose of 1 mg/kg led to smaller litter size and higher incidence of stillbirth. Continued maternal administration of nicotine (100 micrograms/kg/day) until 12 days post partum did not affect newborn growth (body weight and length and size of heart and lung) during the first week after birth; during the second week, however, the nicotine-treated group lagged behind the controls. The stomachs of pups of nicotine-treated rats contained less food than those of controls; this difference increased with age, becoming more than 40% at 12 days. We suggest that lower milk production of nicotine-treated rats interferes with the normal development of the offspring during periods of rapid growth.", "contents": "Effect of nicotine on the development of fetal and suckling rats. Nicotine, administered at a dose of 100 micrograms/kg/day from day 14 of gestation, did not affect maternal food intake, weight gain, length of gestation, litter size or fetal development; however, a daily dose of 1 mg/kg led to smaller litter size and higher incidence of stillbirth. Continued maternal administration of nicotine (100 micrograms/kg/day) until 12 days post partum did not affect newborn growth (body weight and length and size of heart and lung) during the first week after birth; during the second week, however, the nicotine-treated group lagged behind the controls. The stomachs of pups of nicotine-treated rats contained less food than those of controls; this difference increased with age, becoming more than 40% at 12 days. We suggest that lower milk production of nicotine-treated rats interferes with the normal development of the offspring during periods of rapid growth."}
{"id": "PMID:476220", "title": "The effect of tricyclic antidepressants on cerebral fluid dynamics.", "content": "Tricyclic antidepressants are thought to act primarily via effects on adrenergic neurotransmitters. Recent research supports the concept that a major function of the central adrenergic system is the modulation of cerebral fluid dynamics. Based on this concept, studies in the rat were conducted to assess the effects of these drugs on cerebral capillary permeability and flow by quantitating changes in the extraction fraction of water (Ew). Amitriptyline and nortriptyline produced significant increased in Ew for the total forebrain (from control values of 0.67 to experimental values as high as 0.99) while protriptyline had no effect on Ew. The amitriptyline-induced increase in Ew occurred at doses which produced plasma levels (500 ng/ml) near the range defined as therapeutic in depression studies. The magnitude of the effect was similar for both amitriptyline and nortriptyline representing a 35--40% increase over control values. The effects were uniformly observed throughout the forebrain: rostral telencephalon, caudal telencephalon, and diencephalon.", "contents": "The effect of tricyclic antidepressants on cerebral fluid dynamics. Tricyclic antidepressants are thought to act primarily via effects on adrenergic neurotransmitters. Recent research supports the concept that a major function of the central adrenergic system is the modulation of cerebral fluid dynamics. Based on this concept, studies in the rat were conducted to assess the effects of these drugs on cerebral capillary permeability and flow by quantitating changes in the extraction fraction of water (Ew). Amitriptyline and nortriptyline produced significant increased in Ew for the total forebrain (from control values of 0.67 to experimental values as high as 0.99) while protriptyline had no effect on Ew. The amitriptyline-induced increase in Ew occurred at doses which produced plasma levels (500 ng/ml) near the range defined as therapeutic in depression studies. The magnitude of the effect was similar for both amitriptyline and nortriptyline representing a 35--40% increase over control values. The effects were uniformly observed throughout the forebrain: rostral telencephalon, caudal telencephalon, and diencephalon."}
{"id": "PMID:476221", "title": "Calcium: bivalent cation in the bivalent psychoses.", "content": "Decreases in cerebrospinal fluid (CSF) calcium accompany mood elevation and motor activation in depressed patients undergoing treatment with ECT, lithium, and total sleep deprivation. Similarly, decreases in CSF calcium occur during acute psychotic agitation or mania. On the other hand, periodic recurrences of such agitated states are accompanied at their onset by transient increases in serum calcium and phosphorus. Several observations suggest that such serum ion shifts may trigger the more enduring and opposite shifts in CSF calcium and, in turn, the manic behavior. Progressive restriction of dietary calcium was earlier reported to mitigate and finally abolish both rhythmic rises in serum calcium and periodic agitated episodes in one psychotic patient. Lithium, which decreases the efficiency of alimentary calcium absorption, may function similarly. Conversely, a modest oral calcium lactate supplement (approximately one additional Recommended Daily Allowance of dietary calcium) seemed to slightly intensify agitation in six patients. Dihydrotachysterol (DHT), an analogue of vitamin D, which more exactly mimics the increase in both serum calcium and phosphorus, appeared in at least one periodically psychotic patient to trigger and opposite shift in CSF calcium. Moreover, in eight patients, manic symptomatology appeared de novo or grew significantly and substantially worse during 2 to 6 weeks of oral DHT administration. On the other hand, in 12 patients, subcutaneous injections of synthetic salmon calcitonin (SCT) decreased serum calcium and phosphorus, increased CSF calcium, and decreased agitation while augmenting depressive symptomatology. SCT also decreased quantified motor activity, frequency and severity of periodic agitated episodes, serum CPK and prolactin, and nocturnal sleep, while DHT or calcium lactate had opposite effects on the same parameters.", "contents": "Calcium: bivalent cation in the bivalent psychoses. Decreases in cerebrospinal fluid (CSF) calcium accompany mood elevation and motor activation in depressed patients undergoing treatment with ECT, lithium, and total sleep deprivation. Similarly, decreases in CSF calcium occur during acute psychotic agitation or mania. On the other hand, periodic recurrences of such agitated states are accompanied at their onset by transient increases in serum calcium and phosphorus. Several observations suggest that such serum ion shifts may trigger the more enduring and opposite shifts in CSF calcium and, in turn, the manic behavior. Progressive restriction of dietary calcium was earlier reported to mitigate and finally abolish both rhythmic rises in serum calcium and periodic agitated episodes in one psychotic patient. Lithium, which decreases the efficiency of alimentary calcium absorption, may function similarly. Conversely, a modest oral calcium lactate supplement (approximately one additional Recommended Daily Allowance of dietary calcium) seemed to slightly intensify agitation in six patients. Dihydrotachysterol (DHT), an analogue of vitamin D, which more exactly mimics the increase in both serum calcium and phosphorus, appeared in at least one periodically psychotic patient to trigger and opposite shift in CSF calcium. Moreover, in eight patients, manic symptomatology appeared de novo or grew significantly and substantially worse during 2 to 6 weeks of oral DHT administration. On the other hand, in 12 patients, subcutaneous injections of synthetic salmon calcitonin (SCT) decreased serum calcium and phosphorus, increased CSF calcium, and decreased agitation while augmenting depressive symptomatology. SCT also decreased quantified motor activity, frequency and severity of periodic agitated episodes, serum CPK and prolactin, and nocturnal sleep, while DHT or calcium lactate had opposite effects on the same parameters."}
{"id": "PMID:476222", "title": "Dopamine-beta-hydroxylase in the cerebrospinal fluid: relationship to disulfiram-induced psychosis.", "content": "Cerebrospinal fluid (CSF) dopamine-beta-hydroxylase (DBH) activity in 32 male alcoholics was measured using a modification of the radioenzymatic method of Molinoff et al. In most, the CSF was obtained before treatment with disulfiram, while in others it was obtained while they were on the drug (250 or 500 mg). As expected, treatment with this reversible DBH inhibitor had no effect on the activity of the enzyme measured in our in vitro assay. However, low pretreatment DBH activity was found to correlate with adverse reactions to disulfiram. Mean DBH activity of four individuals who went on to become psychotic on disulfiram was 0.13 +/- 0.02 nmole/ml per hr (mean +/- SEM). An additional four individuals who developed dysphoric but nonpsychotic reactions had a mean DBH of 0.23 +/- 0.03. Both these values were significantly lower than the mean DBH activity of the remaining 24 individuals treated with disulfiram who had no adverse side effects, 0.53 +/- 0.06 p less than 0.02 and p less than 0.05, respectively, 2-tailed t-test.", "contents": "Dopamine-beta-hydroxylase in the cerebrospinal fluid: relationship to disulfiram-induced psychosis. Cerebrospinal fluid (CSF) dopamine-beta-hydroxylase (DBH) activity in 32 male alcoholics was measured using a modification of the radioenzymatic method of Molinoff et al. In most, the CSF was obtained before treatment with disulfiram, while in others it was obtained while they were on the drug (250 or 500 mg). As expected, treatment with this reversible DBH inhibitor had no effect on the activity of the enzyme measured in our in vitro assay. However, low pretreatment DBH activity was found to correlate with adverse reactions to disulfiram. Mean DBH activity of four individuals who went on to become psychotic on disulfiram was 0.13 +/- 0.02 nmole/ml per hr (mean +/- SEM). An additional four individuals who developed dysphoric but nonpsychotic reactions had a mean DBH of 0.23 +/- 0.03. Both these values were significantly lower than the mean DBH activity of the remaining 24 individuals treated with disulfiram who had no adverse side effects, 0.53 +/- 0.06 p less than 0.02 and p less than 0.05, respectively, 2-tailed t-test."}
{"id": "PMID:476223", "title": "d-Amphetamine: effects on memory in a depressed population.", "content": "The effect of intravenous d-amphetamine on memory functions in a group of depressed patients was examined in a double-blind placebo-controlled study. Active drug administration resulted in an increase in verbal free recall but no change in cued recall, suggesting specific effects on memory processes. The level of psychological processing of the presented stimulus was shown to interact with drug-induced facilitation of recall. Improvement in memory of more shallowly processed material under amphetamine related significantly to subjects' base-line indices of noradrenergic function. Drug-induced changes in mood did not correlate with improvement in cognitive functioning. The interrelationships between biochemical determinants of mood and memory are discussed in light of these findings.", "contents": "d-Amphetamine: effects on memory in a depressed population. The effect of intravenous d-amphetamine on memory functions in a group of depressed patients was examined in a double-blind placebo-controlled study. Active drug administration resulted in an increase in verbal free recall but no change in cued recall, suggesting specific effects on memory processes. The level of psychological processing of the presented stimulus was shown to interact with drug-induced facilitation of recall. Improvement in memory of more shallowly processed material under amphetamine related significantly to subjects' base-line indices of noradrenergic function. Drug-induced changes in mood did not correlate with improvement in cognitive functioning. The interrelationships between biochemical determinants of mood and memory are discussed in light of these findings."}
{"id": "PMID:476224", "title": "Somatosensory and auditory evoked potential studies of functional differences between the cerebral hemispheres in psychosis.", "content": "Evidence of lateralized hemispheric dysfunction in schizophrenia was previously found with a measure of visual evoked potential (VEP) wave-shape stability. The purpose of this study was to determine whether the VEP findings would extend to the auditory and somatosensory modalities. Measures of wave-shape stability were computed for auditory (AEP) and somatosensory (SEP) evoked potentials, recorded from one EOG and 14 scalp leads. Subjects were 74 unmedicated patients (49 schizophrenics, 25 nonpsychotics) and 27 nonpatients; two sets of age- and sex-matched comparison groups were formed. Main findings were: (i) Overtly psychotic schizophrenics exhibited lower than normal stability in left hemisphere AEPs (15-100 msec poststimulus). (ii) Both overtly psychotic and latent schizophrenics showed lower than normal wave-form stability, bilaterally, in the later epoch of AEPs (101-450 msec poststimulus). (iii) A subgroup of overt schizophrenics (other than chronic paranoid or chronic undifferentiated) had lower than normal stability in SEPs (15-100 msec) evoked by right median nerve stimuli and recorded from the right (ipsilateral) hemisphere. It was concluded that only the AEP results augment the previous VEP evidence indicating left hemisphere involvement in schizophrenic dysfunction.", "contents": "Somatosensory and auditory evoked potential studies of functional differences between the cerebral hemispheres in psychosis. Evidence of lateralized hemispheric dysfunction in schizophrenia was previously found with a measure of visual evoked potential (VEP) wave-shape stability. The purpose of this study was to determine whether the VEP findings would extend to the auditory and somatosensory modalities. Measures of wave-shape stability were computed for auditory (AEP) and somatosensory (SEP) evoked potentials, recorded from one EOG and 14 scalp leads. Subjects were 74 unmedicated patients (49 schizophrenics, 25 nonpsychotics) and 27 nonpatients; two sets of age- and sex-matched comparison groups were formed. Main findings were: (i) Overtly psychotic schizophrenics exhibited lower than normal stability in left hemisphere AEPs (15-100 msec poststimulus). (ii) Both overtly psychotic and latent schizophrenics showed lower than normal wave-form stability, bilaterally, in the later epoch of AEPs (101-450 msec poststimulus). (iii) A subgroup of overt schizophrenics (other than chronic paranoid or chronic undifferentiated) had lower than normal stability in SEPs (15-100 msec) evoked by right median nerve stimuli and recorded from the right (ipsilateral) hemisphere. It was concluded that only the AEP results augment the previous VEP evidence indicating left hemisphere involvement in schizophrenic dysfunction."}
{"id": "PMID:476225", "title": "Psychological characteristics corresponding to low versus high platelet monoamine oxidase activity.", "content": "From an original sample of 375 college students and employees studied for platelet MAO activity, 66 subjects representing the lower and upper deciles of the sample were contacted for further study, including administration of the booklet form of the MMPI. We analyzed what psychological characteristics might be associated with differences in MAO activity and attempted to cross-validate these characteristics by using them to predict the separability of low vs. high MAO subjects. Each subject was randomly assigned to one of two groups in which the low and high MAO subjects were balanced for sex. For 16 women in group A, a MAO scale discriminated low and high subjects with 100% accuracy. For 18 men in group A, a separate MAO scale discriminated with 94% accuracy. To cross-validate these results, the two scales were applied to another population; both discriminated low and high MAO women and men with a combined 97% accuracy. The thematic content of the two scales is discussed in the light of other reports on the psychological characteristics of low and high MAO subjects, including the apparent relationship between the scale content and the clinical features of bipolar affective disorder.", "contents": "Psychological characteristics corresponding to low versus high platelet monoamine oxidase activity. From an original sample of 375 college students and employees studied for platelet MAO activity, 66 subjects representing the lower and upper deciles of the sample were contacted for further study, including administration of the booklet form of the MMPI. We analyzed what psychological characteristics might be associated with differences in MAO activity and attempted to cross-validate these characteristics by using them to predict the separability of low vs. high MAO subjects. Each subject was randomly assigned to one of two groups in which the low and high MAO subjects were balanced for sex. For 16 women in group A, a MAO scale discriminated low and high subjects with 100% accuracy. For 18 men in group A, a separate MAO scale discriminated with 94% accuracy. To cross-validate these results, the two scales were applied to another population; both discriminated low and high MAO women and men with a combined 97% accuracy. The thematic content of the two scales is discussed in the light of other reports on the psychological characteristics of low and high MAO subjects, including the apparent relationship between the scale content and the clinical features of bipolar affective disorder."}
{"id": "PMID:476226", "title": "Familial biochemical and clinical correlates of alcoholics with low platelet monoamine oxidase activity.", "content": "This study substantiates previous reports that low platelet MAO activity is associated with alcoholism. The results also indicate that low platelet MAO activity in alcoholic probands is associated with a higher prevalence of psychiatric hospitalization in first-degree family members as well as alcoholism and suicide attempts among alcoholics. Psychiatrically ill family members of the alcoholic probands with low platelet MAO activity also demonstrate low enzyme activity whereas the well family members have normal enzyme activity.", "contents": "Familial biochemical and clinical correlates of alcoholics with low platelet monoamine oxidase activity. This study substantiates previous reports that low platelet MAO activity is associated with alcoholism. The results also indicate that low platelet MAO activity in alcoholic probands is associated with a higher prevalence of psychiatric hospitalization in first-degree family members as well as alcoholism and suicide attempts among alcoholics. Psychiatrically ill family members of the alcoholic probands with low platelet MAO activity also demonstrate low enzyme activity whereas the well family members have normal enzyme activity."}
{"id": "PMID:476227", "title": "The mimicking of mania in \"benign\" herpes simplex encephalitis.", "content": "This paper reports on a relative rarity, the case of a 41-year-old male who, after receiving an original admission diagnosis of clear-cut acute mania, actually turned out to be suffering from herpes simplex encephalitis. Interestingly enough, his illness was unusually \"benign\" in the sense that at no time during massive cycling bipolar mood disturbances did any clinically apparent neurological signs and symptoms or other serious somatic complications or any psychopathology pointing to an acute organic brain syndrome appear. Karl Bonhoeffer's often forgotten but still relevant clinical insights on acute organic psychoses are then discussed in the light of recent research attempting to link manic states and infectious illness.", "contents": "The mimicking of mania in \"benign\" herpes simplex encephalitis. This paper reports on a relative rarity, the case of a 41-year-old male who, after receiving an original admission diagnosis of clear-cut acute mania, actually turned out to be suffering from herpes simplex encephalitis. Interestingly enough, his illness was unusually \"benign\" in the sense that at no time during massive cycling bipolar mood disturbances did any clinically apparent neurological signs and symptoms or other serious somatic complications or any psychopathology pointing to an acute organic brain syndrome appear. Karl Bonhoeffer's often forgotten but still relevant clinical insights on acute organic psychoses are then discussed in the light of recent research attempting to link manic states and infectious illness."}
{"id": "PMID:476229", "title": "Succinylcholine drug interactions during electroconvulsive therapy.", "content": "Electroconvulsive therapy (ECT) is frequently described as being almost free of absolute contraindications. However, drug interactions with succinylcholine pose potential hazards to which this paper calls attention. In addition to its neuromuscular-blocking action, succinylcholine has other acetylcholine-like effects. Drugs which inhibit cholinesterase activity or which inhibit acetylcholine release or synthesis may produce prolonged respiratory paralysis when combined with succinylcholine. The increasing longevity of the population receiving chemotherapy for physical ailments implies that greater numbers of these patients may also become candidates for ECT and therefore be at risk for adverse drug interactions with succinylcholine. Of particular importance to the psychiatrist are the various psychotropic drugs that have been reported to interact with succinylcholine. Precautions in using drug combinations and treatment of complications are described.", "contents": "Succinylcholine drug interactions during electroconvulsive therapy. Electroconvulsive therapy (ECT) is frequently described as being almost free of absolute contraindications. However, drug interactions with succinylcholine pose potential hazards to which this paper calls attention. In addition to its neuromuscular-blocking action, succinylcholine has other acetylcholine-like effects. Drugs which inhibit cholinesterase activity or which inhibit acetylcholine release or synthesis may produce prolonged respiratory paralysis when combined with succinylcholine. The increasing longevity of the population receiving chemotherapy for physical ailments implies that greater numbers of these patients may also become candidates for ECT and therefore be at risk for adverse drug interactions with succinylcholine. Of particular importance to the psychiatrist are the various psychotropic drugs that have been reported to interact with succinylcholine. Precautions in using drug combinations and treatment of complications are described."}
{"id": "PMID:476231", "title": "The psychological effects of dopamine-beta-hydroxylase inhibition in normal subjects.", "content": "Acute administration of the dopamine-beta-hydroxylase (DBH) inhibitor, fusaric acid, combined with L-dopa, 500 mg, produced discernible psychological abnormalities in normal subjects. These were not found after L-dopa alone, or after placebo. The changes, lasting a few hours, were manifested by significant increases in \"total psychopathology,\" and in a number of subscales on the Brief Psychiatric Rating Scale. Changes were also found in mood ratings on adjective checklists, and inkblot tests. A variety of effects were found on psychiatric interview. Since DBH does not occur in dopamine neurons, it is likely that the psychological effects were due to changes in dopamine or norepinephrine in norepinephrine neurons.", "contents": "The psychological effects of dopamine-beta-hydroxylase inhibition in normal subjects. Acute administration of the dopamine-beta-hydroxylase (DBH) inhibitor, fusaric acid, combined with L-dopa, 500 mg, produced discernible psychological abnormalities in normal subjects. These were not found after L-dopa alone, or after placebo. The changes, lasting a few hours, were manifested by significant increases in \"total psychopathology,\" and in a number of subscales on the Brief Psychiatric Rating Scale. Changes were also found in mood ratings on adjective checklists, and inkblot tests. A variety of effects were found on psychiatric interview. Since DBH does not occur in dopamine neurons, it is likely that the psychological effects were due to changes in dopamine or norepinephrine in norepinephrine neurons."}
{"id": "PMID:476232", "title": "3-Methoxy-4-hydroxyphenylglycol and primary depression: clinical and pharmacological considerations.", "content": "Pretherapeutic urinary excretion of 3-methoxy-4-hydroxyphenylglycol (MHPG) was studied in 25 primary depressed patients. The results indicate that: (i) There exists a wide variability among primary depressives with respect to MHPG excretion. (ii) Age of onset together with polarity of the disease may account for the nonhomogeneous division of the patients according to MHPG levels. (iii). The other variables considered, with the possible exception of motor activity, do not explain the dichotomy between high or normal and low MHPG levels, even though it is possible that they influence MHPG excretion to some extent, with the consequent possibility of errors in subclassification of the patients at the boundaries between the two groups. (iv) The correlation between motor retardation and low MHPG excretion is positive, but probably due to a frequent association between this motricity state and primary depression of bipolar early onset type. (v) Treatments with chlorimipramine and, to a lesser degree of specificity, with amitriptyline are particularly indicated in patients with normal or high MHPG. Some practical and theoretical implications deriving from these data are briefly discussed.", "contents": "3-Methoxy-4-hydroxyphenylglycol and primary depression: clinical and pharmacological considerations. Pretherapeutic urinary excretion of 3-methoxy-4-hydroxyphenylglycol (MHPG) was studied in 25 primary depressed patients. The results indicate that: (i) There exists a wide variability among primary depressives with respect to MHPG excretion. (ii) Age of onset together with polarity of the disease may account for the nonhomogeneous division of the patients according to MHPG levels. (iii). The other variables considered, with the possible exception of motor activity, do not explain the dichotomy between high or normal and low MHPG levels, even though it is possible that they influence MHPG excretion to some extent, with the consequent possibility of errors in subclassification of the patients at the boundaries between the two groups. (iv) The correlation between motor retardation and low MHPG excretion is positive, but probably due to a frequent association between this motricity state and primary depression of bipolar early onset type. (v) Treatments with chlorimipramine and, to a lesser degree of specificity, with amitriptyline are particularly indicated in patients with normal or high MHPG. Some practical and theoretical implications deriving from these data are briefly discussed."}
{"id": "PMID:476233", "title": "Epileptic phenomena induced in the cat by the antidepressants maprotiline, imipramine, clomipramine, and amitriptyline.", "content": "The epileptogenic properties of four tricyclic antidepressant drugs: maprotiline, imipramine, clomipramine, amitriptyline, were investigated in locally anesthetized cats immobilized with gallamine and supplied with neocortical, hippocampal, and reticular recording electrodes. The drugs were infused intravenously at a constant rate (0.5 or, in some cases, 0.25 mg/kg per min) up to a final dose of 45 mg/kg. Already in small doses (1 to 5 mg/kg) all four antidepressants produced local signs of epileptiform pathology. Generalized sustained discharges occurred, on the average, at between 20 and 25 mg/kg with all four drugs. Imipramine and amitriptyline, after the first or first few generalized discharges, led to a pattern of repeated short generalized seizures alternating with silent periods. Maprotiline invariably produced this later alternating pattern only after a 10- to 30-min period of a seminormal high amplitude pattern. Clomipramine assumed a position between maprotiline on the one hand and imipramine and amitriptyline on the other. Starting at doses of 2-4 mg/kg, imipramine, clomipramine and amitriptyline, all three being norepinephrine and serotonin uptake inhibitors, induced a high amplitude \"sleep\" pattern. Maprotiline, a  norepinephrine uptake inhibitor, which is thought devoid of serotonin-uptake inhibiting properties, led to high amplitude slow waves only with doses of at least 12.5 to 15 mg/kg.", "contents": "Epileptic phenomena induced in the cat by the antidepressants maprotiline, imipramine, clomipramine, and amitriptyline. The epileptogenic properties of four tricyclic antidepressant drugs: maprotiline, imipramine, clomipramine, amitriptyline, were investigated in locally anesthetized cats immobilized with gallamine and supplied with neocortical, hippocampal, and reticular recording electrodes. The drugs were infused intravenously at a constant rate (0.5 or, in some cases, 0.25 mg/kg per min) up to a final dose of 45 mg/kg. Already in small doses (1 to 5 mg/kg) all four antidepressants produced local signs of epileptiform pathology. Generalized sustained discharges occurred, on the average, at between 20 and 25 mg/kg with all four drugs. Imipramine and amitriptyline, after the first or first few generalized discharges, led to a pattern of repeated short generalized seizures alternating with silent periods. Maprotiline invariably produced this later alternating pattern only after a 10- to 30-min period of a seminormal high amplitude pattern. Clomipramine assumed a position between maprotiline on the one hand and imipramine and amitriptyline on the other. Starting at doses of 2-4 mg/kg, imipramine, clomipramine and amitriptyline, all three being norepinephrine and serotonin uptake inhibitors, induced a high amplitude \"sleep\" pattern. Maprotiline, a  norepinephrine uptake inhibitor, which is thought devoid of serotonin-uptake inhibiting properties, led to high amplitude slow waves only with doses of at least 12.5 to 15 mg/kg."}
{"id": "PMID:476256", "title": "Material effects in shear-induced hemolysis.", "content": "A rotating-disk apparatus for shearing blood was employed to compare 22 materials for their tendency to cause hemolysis during standardized low-stress (130 dynes/cm2 maximum) laminar flow conditions. Rigid plastics, hydrogels, and carbons were among the materials tested. Time-independent ranking of the materials was possible for 75% of the data; these rankings correlated linearly (for polymers) with the critical surface tension gammac over the range 20--46 dyne/cm, with low gammac being associated with low hemolysis. Surface morphology was also found to influence hemolysis. Roughness in the 1--15 micron range had a distinct effect on hemolysis kinetics for polyethylene surfaces. This suggest that failure to find time-independence in 25% of the material rankings can be attributed to inadequate control of the roughness variable. It also emphasizes the importance of surface morphology, as well as surface chemistry, as a biocompatibility parameter.", "contents": "Material effects in shear-induced hemolysis. A rotating-disk apparatus for shearing blood was employed to compare 22 materials for their tendency to cause hemolysis during standardized low-stress (130 dynes/cm2 maximum) laminar flow conditions. Rigid plastics, hydrogels, and carbons were among the materials tested. Time-independent ranking of the materials was possible for 75% of the data; these rankings correlated linearly (for polymers) with the critical surface tension gammac over the range 20--46 dyne/cm, with low gammac being associated with low hemolysis. Surface morphology was also found to influence hemolysis. Roughness in the 1--15 micron range had a distinct effect on hemolysis kinetics for polyethylene surfaces. This suggest that failure to find time-independence in 25% of the material rankings can be attributed to inadequate control of the roughness variable. It also emphasizes the importance of surface morphology, as well as surface chemistry, as a biocompatibility parameter."}
{"id": "PMID:476257", "title": "Observations on shear-induced hemolysis.", "content": "New data are reported on chemical and mechanical factors affecting the hemolysis of blood in a rotating-parallel-disk apparatus used in biomaterials studies described elsewhere. The hemolytic potential for a blood is found to correlate with plasma components LDH and total triglycerides existing prior to shear. Antibiotics are shown to suppress hemolysis, unrelated to bacterial action. The kinetics of measured hemoglobin release are analyzed in terms of a red cell population having a distribution of fragilities, sample storage age, and possible hemoglobin adsorption. Blood/gas interfacial effects are examined by varying the gas and the extent of interface, and found to be minimal under normal testing conditions. Several geometric and dynamic parameters are varied to illustrate the complexities of engineering design and scale-up.", "contents": "Observations on shear-induced hemolysis. New data are reported on chemical and mechanical factors affecting the hemolysis of blood in a rotating-parallel-disk apparatus used in biomaterials studies described elsewhere. The hemolytic potential for a blood is found to correlate with plasma components LDH and total triglycerides existing prior to shear. Antibiotics are shown to suppress hemolysis, unrelated to bacterial action. The kinetics of measured hemoglobin release are analyzed in terms of a red cell population having a distribution of fragilities, sample storage age, and possible hemoglobin adsorption. Blood/gas interfacial effects are examined by varying the gas and the extent of interface, and found to be minimal under normal testing conditions. Several geometric and dynamic parameters are varied to illustrate the complexities of engineering design and scale-up."}
{"id": "PMID:476258", "title": "Characterization of homogeneous and pseudocomposite homopolymers and copolymers for articular cartilage replacement.", "content": "Semicrystalline swollen polymeric networks are prime candidates for biomedical applications. Purity, controlled structure, easy preparation, non-biodegradability and improved mechanical properties are some of their most important characteristics. New articular cartilage simulants in the form of hydrogels with surface hydroxyl groups have been developed and characterized. The mechanical properties and physical behavior of these materials are described by high elastic moduli, ultimate tensile strength and elongation at break, low tear propagation resistance and excellent friction properties. Crystallinities vary between 15% and 35% and the crystallites are stable within the range of practical applications. The hydrogels show no permeability to hyaluronic acid and can be grafted with cationic monomers or polymers to form boundary lubricant layers. Techniques have also been developed for the preparation of deformable pseudocomposites consisting of layers of swollen semicrystalline and amorphous polymer.", "contents": "Characterization of homogeneous and pseudocomposite homopolymers and copolymers for articular cartilage replacement. Semicrystalline swollen polymeric networks are prime candidates for biomedical applications. Purity, controlled structure, easy preparation, non-biodegradability and improved mechanical properties are some of their most important characteristics. New articular cartilage simulants in the form of hydrogels with surface hydroxyl groups have been developed and characterized. The mechanical properties and physical behavior of these materials are described by high elastic moduli, ultimate tensile strength and elongation at break, low tear propagation resistance and excellent friction properties. Crystallinities vary between 15% and 35% and the crystallites are stable within the range of practical applications. The hydrogels show no permeability to hyaluronic acid and can be grafted with cationic monomers or polymers to form boundary lubricant layers. Techniques have also been developed for the preparation of deformable pseudocomposites consisting of layers of swollen semicrystalline and amorphous polymer."}
{"id": "PMID:476260", "title": "Comparative studies of hemoperfusion devices. II. Pressure drop and flow uniformity tests.", "content": "One resin-based hemoperfusion device and three charcoal-based hemoperfusion devices were tested to determine their pressure drop and flow uniformity characteristics. Measurements were made on pressure drop versus flow rate using distilled water and on pressure drop versus time using bovine blood. Effluent concentration curves obtained after the step-change introduction of a high molecular weight dye solution to each unit were used to determine the priming volumes of the devices and were interpreted to yield information regarding the uniformities of flow in each device. The pressure drop and priming volume values for the resin-based device were significantly higher than the corresponding values for the charcoal-based units.", "contents": "Comparative studies of hemoperfusion devices. II. Pressure drop and flow uniformity tests. One resin-based hemoperfusion device and three charcoal-based hemoperfusion devices were tested to determine their pressure drop and flow uniformity characteristics. Measurements were made on pressure drop versus flow rate using distilled water and on pressure drop versus time using bovine blood. Effluent concentration curves obtained after the step-change introduction of a high molecular weight dye solution to each unit were used to determine the priming volumes of the devices and were interpreted to yield information regarding the uniformities of flow in each device. The pressure drop and priming volume values for the resin-based device were significantly higher than the corresponding values for the charcoal-based units."}
{"id": "PMID:476264", "title": "Calculation of blood velocity and pressure in stenosed renal artery considered as a Venturi tube.", "content": "The authors propose using a Venturi tube as a model of stenosed renal artery; they can thus calculate blood velocity and pressure in the stenosed part from estimated renal flow, systemic pressure and the hydraulic characteristics of the model.", "contents": "Calculation of blood velocity and pressure in stenosed renal artery considered as a Venturi tube. The authors propose using a Venturi tube as a model of stenosed renal artery; they can thus calculate blood velocity and pressure in the stenosed part from estimated renal flow, systemic pressure and the hydraulic characteristics of the model."}
{"id": "PMID:476266", "title": "Migraine. A platelet hypothesis.", "content": "This paper presents evidence for my hypothesis that the diverse features of a migraine attack, both during the prodromal and the headache phases, become explicable if migraine is caused by a primary abnormality of platelet behaviour. This hypothesis places migraine among the most common disorders of the blood.", "contents": "Migraine. A platelet hypothesis. This paper presents evidence for my hypothesis that the diverse features of a migraine attack, both during the prodromal and the headache phases, become explicable if migraine is caused by a primary abnormality of platelet behaviour. This hypothesis places migraine among the most common disorders of the blood."}
{"id": "PMID:476265", "title": "Further investigations about actinomycin D action on mouse erythropoietin responsive cell.", "content": "Repeated injections of 100 micrograms/kg/day of Actinomycin D entirely eradicated erythropoiesis in normal mice. The study of the effect of graded Actinomycin D doses on the erythropoiesis displayed a reproducible dose-effect relationship. The same relation appeared in the action of various Actinomycin D doses on the response to erythropoietin in hypertransfused-mice. To decide whether Actinomycin D had destructive or inhibitory action on its target cell, myleran was used. A single injection of myleran reduced the pluripotent haematopoietic stem cell (CFU) and the erythropoietin responsive cell (ERC) in polycythemic mice to around 1% that of the controls. When repeated injections of erythropoietin restored a transitory ERC population in the absence of CFU, Actinomycin D to the doses of 90 and 120 micrograms/kg-mouse/day did not prevent the erythropoietin effect on self replication of ERC. This result directly showed that Actinomycin D acted on the ERC by selective inhibition of their differentiation without cytotoxic effect.", "contents": "Further investigations about actinomycin D action on mouse erythropoietin responsive cell. Repeated injections of 100 micrograms/kg/day of Actinomycin D entirely eradicated erythropoiesis in normal mice. The study of the effect of graded Actinomycin D doses on the erythropoiesis displayed a reproducible dose-effect relationship. The same relation appeared in the action of various Actinomycin D doses on the response to erythropoietin in hypertransfused-mice. To decide whether Actinomycin D had destructive or inhibitory action on its target cell, myleran was used. A single injection of myleran reduced the pluripotent haematopoietic stem cell (CFU) and the erythropoietin responsive cell (ERC) in polycythemic mice to around 1% that of the controls. When repeated injections of erythropoietin restored a transitory ERC population in the absence of CFU, Actinomycin D to the doses of 90 and 120 micrograms/kg-mouse/day did not prevent the erythropoietin effect on self replication of ERC. This result directly showed that Actinomycin D acted on the ERC by selective inhibition of their differentiation without cytotoxic effect."}
{"id": "PMID:476267", "title": "Inborn hypouricemia due to isolated renal tubular defect.", "content": "Inborn hypouricemia due to isolated renal tubular defect is a rare disorder. Thus far eight documented families with this condition have been reported. In reviewing the data on these families, hyperuricosuria was found to be a constant associate of the hypouricemia, and hypercalciuria, mainly of the absorptive type, was frequently associated. Urolithiasis appears to be common. The mode of inheritance of this hypouricemia was autosomal, probably recessive. The magnitude of renal urate clearance and the effects on it of probenecid and pyrazinamide suggest the inborn renal hypouricemia to be of two types, due to defective re-secretory tubular urate reabsorption and to total defective tubular urate reabsorption. The question of the renal tubular abnormality for urate transport being the primary defect or secondary to an abnormal metabolite is discussed.", "contents": "Inborn hypouricemia due to isolated renal tubular defect. Inborn hypouricemia due to isolated renal tubular defect is a rare disorder. Thus far eight documented families with this condition have been reported. In reviewing the data on these families, hyperuricosuria was found to be a constant associate of the hypouricemia, and hypercalciuria, mainly of the absorptive type, was frequently associated. Urolithiasis appears to be common. The mode of inheritance of this hypouricemia was autosomal, probably recessive. The magnitude of renal urate clearance and the effects on it of probenecid and pyrazinamide suggest the inborn renal hypouricemia to be of two types, due to defective re-secretory tubular urate reabsorption and to total defective tubular urate reabsorption. The question of the renal tubular abnormality for urate transport being the primary defect or secondary to an abnormal metabolite is discussed."}
{"id": "PMID:476268", "title": "Immuno-heterogeneity of the calcitonins of hypercalcemia, breast and lung cancers and medullary carcinoma of thyroid.", "content": "Four antisera raised in the goat have very different properties: all recognized the immunoreactive calcitonin (iCT) of medullary carcinoma of the thyroid (MCT), one the response of normal subjects to induced or endogenous hypercalcemia and 2 others a different molecular species which occurs in half the patients with cancer of breast and 3/4 of patients with cancer of the lung. The latter two antisera are most sensitive to the 22-32 sequence of human calcitonin. Depending on the antiserum used, 4 or 7 peaks of immunoreactivity are found in eluates by column chromatography or stimulated serum from MCT. Not all elevated levels of iCT in serum are diagnostic of MCT and ectopic production by lung and breast cancer must be considered. Presence of higher levels of iCT with greater amounts of cancer tissue and undetectable levels after surgery or radiotherapy when using antisera which require intact molecule of calcitonin for recognition suggest the possibility that sequential calcitonin levels with differentiating antisera may be helpful in assessing the extent of disease and response to therapy.", "contents": "Immuno-heterogeneity of the calcitonins of hypercalcemia, breast and lung cancers and medullary carcinoma of thyroid. Four antisera raised in the goat have very different properties: all recognized the immunoreactive calcitonin (iCT) of medullary carcinoma of the thyroid (MCT), one the response of normal subjects to induced or endogenous hypercalcemia and 2 others a different molecular species which occurs in half the patients with cancer of breast and 3/4 of patients with cancer of the lung. The latter two antisera are most sensitive to the 22-32 sequence of human calcitonin. Depending on the antiserum used, 4 or 7 peaks of immunoreactivity are found in eluates by column chromatography or stimulated serum from MCT. Not all elevated levels of iCT in serum are diagnostic of MCT and ectopic production by lung and breast cancer must be considered. Presence of higher levels of iCT with greater amounts of cancer tissue and undetectable levels after surgery or radiotherapy when using antisera which require intact molecule of calcitonin for recognition suggest the possibility that sequential calcitonin levels with differentiating antisera may be helpful in assessing the extent of disease and response to therapy."}
{"id": "PMID:476270", "title": "BCG immunotherapy in previously treated malignant melanoma patients.", "content": "Eighteen patients with histologically confirmed metastatic malignant melanoma were treated with intradermal BCG. Before starting immunotherapy their immunocompetence was tested in vivo and in vitro. Four of 15 (27%) achieved complete regression, and two (13%) a regression of more than 50%, after systemic BCG. All three patients treated with perinodular injection of BCG had complete regression of treated as well as some of untreated nodules. Treatment was unsuccessful in 9 patients. Five of them (33%) had a disease stabilisation for more than 5 months. The results of BCG immunotherapy were compared with those of chemotherapy alone. The immunotherapy patients had longer remissions and survived longer than those treated by chemotherapy. All patients had repeat skin tests with PPD after BCG but showed no significant improvement in tuberculin reactivity. Despite, their clinical condition often being improved after BCG. We conclude that BCG may be of considerable benefit to malignant melanoma patients.", "contents": "BCG immunotherapy in previously treated malignant melanoma patients. Eighteen patients with histologically confirmed metastatic malignant melanoma were treated with intradermal BCG. Before starting immunotherapy their immunocompetence was tested in vivo and in vitro. Four of 15 (27%) achieved complete regression, and two (13%) a regression of more than 50%, after systemic BCG. All three patients treated with perinodular injection of BCG had complete regression of treated as well as some of untreated nodules. Treatment was unsuccessful in 9 patients. Five of them (33%) had a disease stabilisation for more than 5 months. The results of BCG immunotherapy were compared with those of chemotherapy alone. The immunotherapy patients had longer remissions and survived longer than those treated by chemotherapy. All patients had repeat skin tests with PPD after BCG but showed no significant improvement in tuberculin reactivity. Despite, their clinical condition often being improved after BCG. We conclude that BCG may be of considerable benefit to malignant melanoma patients."}
{"id": "PMID:476269", "title": "Pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) and various acute phase reactants in rheumatoid arthritis and osteoarthritis.", "content": "Pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) concentrations have been measured in matched sera and synovial fluid samples obtained from 36 patients with rheumatoid arthritis and 10 patients with osteoarthritis. Levels of alpha 2-PAG in serum and synovial fluid were significantly higher in rheumatoid arthritis than in osteoarthritis. Calculation of the synovial fluid/serum ratios for alpha 2-PAG gave results which are explicable only if this protein were being synthesised locally. In a longitudinal study of 15 patients with rheumatoid arthritis, concentrations of alpha 2-PAG did not reflect disease activity, unlike those of the classical acute phase reactants, C-reactive protein and caeruloplasmin.", "contents": "Pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) and various acute phase reactants in rheumatoid arthritis and osteoarthritis. Pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) concentrations have been measured in matched sera and synovial fluid samples obtained from 36 patients with rheumatoid arthritis and 10 patients with osteoarthritis. Levels of alpha 2-PAG in serum and synovial fluid were significantly higher in rheumatoid arthritis than in osteoarthritis. Calculation of the synovial fluid/serum ratios for alpha 2-PAG gave results which are explicable only if this protein were being synthesised locally. In a longitudinal study of 15 patients with rheumatoid arthritis, concentrations of alpha 2-PAG did not reflect disease activity, unlike those of the classical acute phase reactants, C-reactive protein and caeruloplasmin."}
{"id": "PMID:476271", "title": "Decreased calcium content in patients with digoxin intoxication.", "content": "The platelet calcium content, the plasma calcium level and the serum digoxin were examined in 90 individuals divided into the following four groups : I -- patients with digoxin intoxication; II -- treated with digoxin; III -- treated with digoxin and furosemide, and IV -- control subjects. The patients with digoxin intoxication showed a lower platelet calcium content as compared to subjects of the other groups (p less than 0.001), whereas the serum digoxin level was significantly higher than in the patients of groups II and III (p less than 0.001). Since the examination of the platelet calcium level is a relatively simple and rapid procedure, with reliable results, its use as a routine method in the determination of digoxin intoxication is suggested.", "contents": "Decreased calcium content in patients with digoxin intoxication. The platelet calcium content, the plasma calcium level and the serum digoxin were examined in 90 individuals divided into the following four groups : I -- patients with digoxin intoxication; II -- treated with digoxin; III -- treated with digoxin and furosemide, and IV -- control subjects. The patients with digoxin intoxication showed a lower platelet calcium content as compared to subjects of the other groups (p less than 0.001), whereas the serum digoxin level was significantly higher than in the patients of groups II and III (p less than 0.001). Since the examination of the platelet calcium level is a relatively simple and rapid procedure, with reliable results, its use as a routine method in the determination of digoxin intoxication is suggested."}
{"id": "PMID:476272", "title": "Electrophoretic mobility of S\u00e9zary syndrome lymphoid cells.", "content": "The variations in electrophoretic mobilities found in different lymphocyte populations present in circulating blood of three patients with S\u00e9zary syndrome have been studied. Evidence is given for variations in electrophoretic mobilities being a function of the cytological type of the pathological cells found. The existence of a significant increase of \"null\" cells is shown, which seem to correspond to S\u00e9zary cells.", "contents": "Electrophoretic mobility of S\u00e9zary syndrome lymphoid cells. The variations in electrophoretic mobilities found in different lymphocyte populations present in circulating blood of three patients with S\u00e9zary syndrome have been studied. Evidence is given for variations in electrophoretic mobilities being a function of the cytological type of the pathological cells found. The existence of a significant increase of \"null\" cells is shown, which seem to correspond to S\u00e9zary cells."}
{"id": "PMID:476273", "title": "The suicide technique in vitro for granulocytic progenitor cells (CFC) by application of hydroxyurea (HU) to low concentrations of human bone marrow cells.", "content": "A suicide technique applicable to human bone marrow granulocytic progenitor cells has been previously described (3). This study discusses the conditions for its use on bone marrow aspirates poor in cells. When the number of cells exposed to hydroxyurea (HU) is proportional to the quantity of HU it is possible to avoid artifacts due to HU toxicity. This technique can repeatedly reproduce a 40--50% suicide. To obtain this result we suggest using a 6.10(-6) mM/ml/cell HU concentration.", "contents": "The suicide technique in vitro for granulocytic progenitor cells (CFC) by application of hydroxyurea (HU) to low concentrations of human bone marrow cells. A suicide technique applicable to human bone marrow granulocytic progenitor cells has been previously described (3). This study discusses the conditions for its use on bone marrow aspirates poor in cells. When the number of cells exposed to hydroxyurea (HU) is proportional to the quantity of HU it is possible to avoid artifacts due to HU toxicity. This technique can repeatedly reproduce a 40--50% suicide. To obtain this result we suggest using a 6.10(-6) mM/ml/cell HU concentration."}
{"id": "PMID:476274", "title": "On the mechanism of the tetrahydropteridine cofactor oxidation in aerobic and H2O2-peroxydase media.", "content": "It is commonly postulated that the enzymatic hydroxylation of phenylalanine, tyrosine and tryptophan involves the concomitant oxidation of a tetrahydropteridinic cofactor to an unstable quinonoid product, converted to the initial compound under the catalytic action of dihydropteridine reductase. We now report UV, NMR, mass spectrum and spectroscopic studies of 2-amino-4-hydroxy-6,7-dimethyl-5, 6, 7, 8-tetrahydropteridine oxidation process either by atmospheric O2 or by the H2O2-peroxidase system. No quinonoid form was visualized and, moreover, the spectral characteristics of UV absorbance spectra, initially reported as specific for the quinonoid form, are related to other oxidation products whose formation is explained here.", "contents": "On the mechanism of the tetrahydropteridine cofactor oxidation in aerobic and H2O2-peroxydase media. It is commonly postulated that the enzymatic hydroxylation of phenylalanine, tyrosine and tryptophan involves the concomitant oxidation of a tetrahydropteridinic cofactor to an unstable quinonoid product, converted to the initial compound under the catalytic action of dihydropteridine reductase. We now report UV, NMR, mass spectrum and spectroscopic studies of 2-amino-4-hydroxy-6,7-dimethyl-5, 6, 7, 8-tetrahydropteridine oxidation process either by atmospheric O2 or by the H2O2-peroxidase system. No quinonoid form was visualized and, moreover, the spectral characteristics of UV absorbance spectra, initially reported as specific for the quinonoid form, are related to other oxidation products whose formation is explained here."}
{"id": "PMID:476278", "title": "Inhibition of murine bone marrow colonies growth by adenosine containing nucleotides.", "content": "The effect of various compounds: NAD+, NADP+, Adenosine Diphospho-Ribose, Nicotinamide, Ribose 5 Phosphate, Adenosine, cAMP, cGMP, on the growth of granulocytic and erythroid mouse bone marrow colonies (CFUc and CFUE) was evaluated. The substances were either added directly to the semi-solid culture system, to obtain a continuous exposure during colony growth, or preincubated for various times with bone marrow cells before plating. The results indicate an inhibitory effect on the number of both colonies of several adenosine containing compounds. The effect is concentration dependent and irreversible. CFUE and CFUC show different sensitivity to the inhibition.", "contents": "Inhibition of murine bone marrow colonies growth by adenosine containing nucleotides. The effect of various compounds: NAD+, NADP+, Adenosine Diphospho-Ribose, Nicotinamide, Ribose 5 Phosphate, Adenosine, cAMP, cGMP, on the growth of granulocytic and erythroid mouse bone marrow colonies (CFUc and CFUE) was evaluated. The substances were either added directly to the semi-solid culture system, to obtain a continuous exposure during colony growth, or preincubated for various times with bone marrow cells before plating. The results indicate an inhibitory effect on the number of both colonies of several adenosine containing compounds. The effect is concentration dependent and irreversible. CFUE and CFUC show different sensitivity to the inhibition."}
{"id": "PMID:476277", "title": "Kidney 15-hydroxy-prostaglandin-dehydrogenase activity during the development of experimental hypertension in the rat.", "content": "15-hydroxy-prostaglandin-dehydrogenase (PGDH) activity was studied in rat kidney homogenates during the development of hypertension, within 20 days after left renal artery constriction by a solid silver clip. In the ischemic kidney PGDH activity increased at day 6, reached maximum at day 10, then progressively at day 15 and returned to normal levels at day 20. No difference was found between contralateral kidneys and kidneys of normotensive control rats. Variations of PGDH activity did not seem to be related to either renal perfusion pressure or renin production. Increased PGDH activity may be a consequence of an enzyme induction following increased PG-synthetase activity, or it could be viewed as a defence mechanism, according to the hypothesis of a prohypertensive effect of PG in the rat.", "contents": "Kidney 15-hydroxy-prostaglandin-dehydrogenase activity during the development of experimental hypertension in the rat. 15-hydroxy-prostaglandin-dehydrogenase (PGDH) activity was studied in rat kidney homogenates during the development of hypertension, within 20 days after left renal artery constriction by a solid silver clip. In the ischemic kidney PGDH activity increased at day 6, reached maximum at day 10, then progressively at day 15 and returned to normal levels at day 20. No difference was found between contralateral kidneys and kidneys of normotensive control rats. Variations of PGDH activity did not seem to be related to either renal perfusion pressure or renin production. Increased PGDH activity may be a consequence of an enzyme induction following increased PG-synthetase activity, or it could be viewed as a defence mechanism, according to the hypothesis of a prohypertensive effect of PG in the rat."}
{"id": "PMID:476279", "title": "Adverse effects of interferon treatment on the life span of NZB mice.", "content": "NZB mice treated with interferon from birth and for over a year, display early some characteristics of the autoimmune disease. In these animals, mortality is clearly higher than in controls. Their death occurs 3 to 6 months before that of mice injected with a \"mock\" preparation.", "contents": "Adverse effects of interferon treatment on the life span of NZB mice. NZB mice treated with interferon from birth and for over a year, display early some characteristics of the autoimmune disease. In these animals, mortality is clearly higher than in controls. Their death occurs 3 to 6 months before that of mice injected with a \"mock\" preparation."}
{"id": "PMID:476281", "title": "Determination of free glycerol on human skin surface.", "content": "In water soluble substances obtained by water extraction from human skin surface, free glycerol was detected by gas chromatography mass spectrometry. A selected ion monitoring method was employed to measure low concentration of free glycerol and the mean amounts were found to be about 0.7 microgram cm-2 on the cheek and 0.2 microgram cm-2 on the forearm and sole.", "contents": "Determination of free glycerol on human skin surface. In water soluble substances obtained by water extraction from human skin surface, free glycerol was detected by gas chromatography mass spectrometry. A selected ion monitoring method was employed to measure low concentration of free glycerol and the mean amounts were found to be about 0.7 microgram cm-2 on the cheek and 0.2 microgram cm-2 on the forearm and sole."}
{"id": "PMID:476280", "title": "Comparison of radioimmunoassay and gas chromatographic mass spectrometric assay for d-amphetamine.", "content": "Quantification of low levels of psychotropic drugs (10(-7) to 10(-9) g ml-1) in small volumes of plasma requires sensitive and accurate methods. Validation of these methods is best achieved by comparing results obtained using several techniques. In this study, amphetamine levels in plasma were measured using gas chromatography mass spectrometry and radioimmunoassay. Correlation of the results obtained by the two methods was found to be positive and high (R = 0.9822). The average coefficient of variation between assays for gas chromatography mass spectrometry was 5.8% and for radioimmunoassay was 12.3%, while the average coefficient of variation within assays for gas chromatography mass spectrometry was 4.9% and for radioimmunoassay 6.9%. Although gas chromatography mass spectrometry was 1.9 times more sensitive than radioimmunoassay, for most purposes, the convenience of the radioimmunoassay method outweights the technical superiority of gas chromatography mass spectrometry.", "contents": "Comparison of radioimmunoassay and gas chromatographic mass spectrometric assay for d-amphetamine. Quantification of low levels of psychotropic drugs (10(-7) to 10(-9) g ml-1) in small volumes of plasma requires sensitive and accurate methods. Validation of these methods is best achieved by comparing results obtained using several techniques. In this study, amphetamine levels in plasma were measured using gas chromatography mass spectrometry and radioimmunoassay. Correlation of the results obtained by the two methods was found to be positive and high (R = 0.9822). The average coefficient of variation between assays for gas chromatography mass spectrometry was 5.8% and for radioimmunoassay was 12.3%, while the average coefficient of variation within assays for gas chromatography mass spectrometry was 4.9% and for radioimmunoassay 6.9%. Although gas chromatography mass spectrometry was 1.9 times more sensitive than radioimmunoassay, for most purposes, the convenience of the radioimmunoassay method outweights the technical superiority of gas chromatography mass spectrometry."}
{"id": "PMID:476283", "title": "A sensitive method for the simultaneous determination in biological fluids of imipramine and desipramine or clomipramine and N-desmethylclomipramine by gas chromatography mass spectrometry.", "content": "A procedure is described which permits the simultaneous determination of imipramine and desipramine or clomipramine and N-desmethylclomipramine in serum or plasma for concentrations in the range of 1-200 ng ml-1. Detection limits of 0.2 ng ml-1 for imipramine and 0.1 ng ml-1 for desipramine were demonstrated with a signal-to-noise ratio maintained at 2:1 or better. The method relies on the derivatization of the secondary amines with heptafluorobutyric anhydride and is based on the combined use of gas chromatography, electron impact mass spectrometry and computerized data handling. The assaying procedure is specific, accurate and precise. It is suitable for routine analyses and has sufficient sensitivity to permit monitoring the drug and metabolite levels in human plasma or serum resulting from a single therapeutic dose.", "contents": "A sensitive method for the simultaneous determination in biological fluids of imipramine and desipramine or clomipramine and N-desmethylclomipramine by gas chromatography mass spectrometry. A procedure is described which permits the simultaneous determination of imipramine and desipramine or clomipramine and N-desmethylclomipramine in serum or plasma for concentrations in the range of 1-200 ng ml-1. Detection limits of 0.2 ng ml-1 for imipramine and 0.1 ng ml-1 for desipramine were demonstrated with a signal-to-noise ratio maintained at 2:1 or better. The method relies on the derivatization of the secondary amines with heptafluorobutyric anhydride and is based on the combined use of gas chromatography, electron impact mass spectrometry and computerized data handling. The assaying procedure is specific, accurate and precise. It is suitable for routine analyses and has sufficient sensitivity to permit monitoring the drug and metabolite levels in human plasma or serum resulting from a single therapeutic dose."}
{"id": "PMID:476282", "title": "Determination of uracil and thymine and their nucleosides and nucleotides in picomole amounts by gas chromatography mass spectrometry selected ion monitoring.", "content": "A stable isotope dilution method is presented by which uracil (Ura) and thymine (Thy) can be determined with high precision and sensitivity at the picomole level utilizing stable isotope dilution and gas chromatography electron impact mass spectrometry in the selected ion monitoring mode. [15N2]Ura and [2H3]Thy served as internal standards. The molecular ions as well as the [M-CH3]+ ion fragments of silylated Ura and Thy (Ura-TMS and Thy-TMS) were suitable for the assay which provides evidence of specificity, if identical results are obtained at both ions. Nucleosides and nucleotides of Ura and Thy were determined following quantitative hydrolysis in 6 N HCl at 180 degrees C for two hours. Other hydrolysis procedures did not give satisfactory results. Levels of free Ura and Thy were measured in human and rat plasma after solvent extraction with a sensitivity of 20-40 pm ml-1 demonstrating ready applicability of the assay method to biological samples. The potential physiological role of circulating Ura and Thy is discussed.", "contents": "Determination of uracil and thymine and their nucleosides and nucleotides in picomole amounts by gas chromatography mass spectrometry selected ion monitoring. A stable isotope dilution method is presented by which uracil (Ura) and thymine (Thy) can be determined with high precision and sensitivity at the picomole level utilizing stable isotope dilution and gas chromatography electron impact mass spectrometry in the selected ion monitoring mode. [15N2]Ura and [2H3]Thy served as internal standards. The molecular ions as well as the [M-CH3]+ ion fragments of silylated Ura and Thy (Ura-TMS and Thy-TMS) were suitable for the assay which provides evidence of specificity, if identical results are obtained at both ions. Nucleosides and nucleotides of Ura and Thy were determined following quantitative hydrolysis in 6 N HCl at 180 degrees C for two hours. Other hydrolysis procedures did not give satisfactory results. Levels of free Ura and Thy were measured in human and rat plasma after solvent extraction with a sensitivity of 20-40 pm ml-1 demonstrating ready applicability of the assay method to biological samples. The potential physiological role of circulating Ura and Thy is discussed."}
{"id": "PMID:476284", "title": "Plasma quantitation of warfarin and warfarin alcohol by gas chromatography chemical ionization mass spectrometry in patients on warfarin maintenance therapy.", "content": "A quantitative method has been developed to measure plasma concentrations of warfarin and warfarin alcohol. The analytical procedure uses deuterated analogues as internal standards, and the technique of selected ion monitoring following gas chromatography methane chemical ionization mass spectrometry of the 4'-methyl ethers of warfarin and warfarin alcohol. Concentrations of warfarin and warfarin alcohol have been measured in plasma samples from 43 patients maintained on chronic warfarin therapy and compared with the 'apparent warfarin' concentration as measured by a fluorometric procedure. The study demonstrated a high degree of correlation between the gas chromatography mass spectrometric derived sum of the individual concentrations of warfarin and warfarin alcohol, and the 'apparent warfarin' concentration determined from a spectrofluorometric assay.", "contents": "Plasma quantitation of warfarin and warfarin alcohol by gas chromatography chemical ionization mass spectrometry in patients on warfarin maintenance therapy. A quantitative method has been developed to measure plasma concentrations of warfarin and warfarin alcohol. The analytical procedure uses deuterated analogues as internal standards, and the technique of selected ion monitoring following gas chromatography methane chemical ionization mass spectrometry of the 4'-methyl ethers of warfarin and warfarin alcohol. Concentrations of warfarin and warfarin alcohol have been measured in plasma samples from 43 patients maintained on chronic warfarin therapy and compared with the 'apparent warfarin' concentration as measured by a fluorometric procedure. The study demonstrated a high degree of correlation between the gas chromatography mass spectrometric derived sum of the individual concentrations of warfarin and warfarin alcohol, and the 'apparent warfarin' concentration determined from a spectrofluorometric assay."}
{"id": "PMID:476285", "title": "Identification of urinary metabolites of flavanone in the rat.", "content": "The urinary metabolites of flavanone in the rate were investigated using a gas chromatographic mass spectrometric method employing an OV-1 capillary column. Forty-three metabolites were detected and the most prominent of these were identified. The most common metabolic reactions were reduction of the keto group and hydroxylation at the 3- or 6-positions. Little hydroxylation occurred in ring B. The major metabolites were flavan-4 alpha-ol, trans-3-hydroxyflavan-4 beta-ol, 6-hydroxyflavanone and 6-hydroxyflavan-4 beta-ol. Gas chromatography analysis of underivatized flavanone metabolites, especially with stainless steel columns, results in the formation of dehydrated products including flavone, flav-3-ene and flavanone itself.", "contents": "Identification of urinary metabolites of flavanone in the rat. The urinary metabolites of flavanone in the rate were investigated using a gas chromatographic mass spectrometric method employing an OV-1 capillary column. Forty-three metabolites were detected and the most prominent of these were identified. The most common metabolic reactions were reduction of the keto group and hydroxylation at the 3- or 6-positions. Little hydroxylation occurred in ring B. The major metabolites were flavan-4 alpha-ol, trans-3-hydroxyflavan-4 beta-ol, 6-hydroxyflavanone and 6-hydroxyflavan-4 beta-ol. Gas chromatography analysis of underivatized flavanone metabolites, especially with stainless steel columns, results in the formation of dehydrated products including flavone, flav-3-ene and flavanone itself."}
{"id": "PMID:476286", "title": "A comparison of calculation procedures for isotope dilution determinations using gas chromatography mass spectrometry.", "content": "Several data reduction procedures have been used for converting isotope ratios, determined using gas chromatography mass spectrometry, into mole ratios for the purpose of quantitating organic compounds. In this report, these procedures are shown to be related under certain limiting approximations of original sample and internal standard isotope ratios. With the understanding that some of the procedures involve approximations, the incorporation of related systematic errors into calculated mole ratio values was investigated. The results indicate that certain data reduction procedures allow quantitation over wider mole ratio ranges and with internal standards which contain a significant quantity of unlabeled impurity.", "contents": "A comparison of calculation procedures for isotope dilution determinations using gas chromatography mass spectrometry. Several data reduction procedures have been used for converting isotope ratios, determined using gas chromatography mass spectrometry, into mole ratios for the purpose of quantitating organic compounds. In this report, these procedures are shown to be related under certain limiting approximations of original sample and internal standard isotope ratios. With the understanding that some of the procedures involve approximations, the incorporation of related systematic errors into calculated mole ratio values was investigated. The results indicate that certain data reduction procedures allow quantitation over wider mole ratio ranges and with internal standards which contain a significant quantity of unlabeled impurity."}
{"id": "PMID:476287", "title": "Selected ion monitoring of glycospingolipid mixtures. Identification of several blood group type glycolipids in the small intestine of an individual rabbit.", "content": "A novel application of selected ion monitoring was used for a mixture of non-acid glycosphingolipids of one rabbit small intestine. Earlier studies of permethylated and permethylated-reduced (LiAIH4) derivatives of model compounds have revealed a specificity and abundance of saccharide ions (terminal monosaccharide(s), disaccharide, trisaccharide, etc., and all sugars plus fatty acid) and of ceramide fragments that permit a conclusive detection of separate glycolipid species in a mixture. The sample (50-200 micrograms) was evaporated slowly (1-5 degrees C min-1 from 150-350 degrees C) from the direct inlet probe of an MS 902 mass spectrometer (electron ionization). Mass spectra with fragments up to about m/z 200 were collected on-line by a computer system. A successive partial separation was obtained for glycolipids with from one up to seven sugars. The structures of eight different compounds were identified. They all had 16:0, 22:0 and 24:0 2-hydroxy fatty acids and 18:0 trihydroxy base (phytosphingosine) as major ceramide components. The dominating complex glycolipid was a hexaglycosylceramide with a blood group B type of sequence. A blood group A type sequence was found in a second hexaglycosylceramide. In support of this, the native mixture showed blood group A and B activity. An intense peak, m/z 182, collected from methylated derivatives were evidence for a dominating type 2 carbohydrate chain of the core tetrasaccharide.", "contents": "Selected ion monitoring of glycospingolipid mixtures. Identification of several blood group type glycolipids in the small intestine of an individual rabbit. A novel application of selected ion monitoring was used for a mixture of non-acid glycosphingolipids of one rabbit small intestine. Earlier studies of permethylated and permethylated-reduced (LiAIH4) derivatives of model compounds have revealed a specificity and abundance of saccharide ions (terminal monosaccharide(s), disaccharide, trisaccharide, etc., and all sugars plus fatty acid) and of ceramide fragments that permit a conclusive detection of separate glycolipid species in a mixture. The sample (50-200 micrograms) was evaporated slowly (1-5 degrees C min-1 from 150-350 degrees C) from the direct inlet probe of an MS 902 mass spectrometer (electron ionization). Mass spectra with fragments up to about m/z 200 were collected on-line by a computer system. A successive partial separation was obtained for glycolipids with from one up to seven sugars. The structures of eight different compounds were identified. They all had 16:0, 22:0 and 24:0 2-hydroxy fatty acids and 18:0 trihydroxy base (phytosphingosine) as major ceramide components. The dominating complex glycolipid was a hexaglycosylceramide with a blood group B type of sequence. A blood group A type sequence was found in a second hexaglycosylceramide. In support of this, the native mixture showed blood group A and B activity. An intense peak, m/z 182, collected from methylated derivatives were evidence for a dominating type 2 carbohydrate chain of the core tetrasaccharide."}
{"id": "PMID:476288", "title": "A quantitative selected ion monitoring assay for ketoprofen determination in human biological fluids.", "content": "A new very sensitive method of ketoprofen [(benzoyl-3-phenyl) 2-propionic acid] determination in various biological fluids using selected ion monitoring is described. After ether extraction is acidic medium, ketoprofen and (benzoyl-4-phenyl)-2-butyric acid used as internal standard are methylated and their concentrations determined by monitoring the m/z values correponding to their respective molecular ions. Standard deviation is below 5% at 100 ng ml-1. Detection limits range from 10 ng ml-1 for urine and serum samples to 50 ng ml-1 for maternal milk specimens. This method has been applied successfully to various biological studies: pharmacokinetic profiles, bioavailability and transfer of the drug through physiological barriers.", "contents": "A quantitative selected ion monitoring assay for ketoprofen determination in human biological fluids. A new very sensitive method of ketoprofen [(benzoyl-3-phenyl) 2-propionic acid] determination in various biological fluids using selected ion monitoring is described. After ether extraction is acidic medium, ketoprofen and (benzoyl-4-phenyl)-2-butyric acid used as internal standard are methylated and their concentrations determined by monitoring the m/z values correponding to their respective molecular ions. Standard deviation is below 5% at 100 ng ml-1. Detection limits range from 10 ng ml-1 for urine and serum samples to 50 ng ml-1 for maternal milk specimens. This method has been applied successfully to various biological studies: pharmacokinetic profiles, bioavailability and transfer of the drug through physiological barriers."}
{"id": "PMID:476289", "title": "Metabolites of loperamide in rats.", "content": "Following intraperitoneal administration to rats of [14C]loperamide, [carbonyl-14] 4-(p-chlorophenyl)-4-hydroxy-N,N-dimethyl-alpha, alpha-diphenyl-1-piperidine butyramide, metabolites in feces and urine were separated, and identified by means of mass spectrometry. In feces, six metabolites were identified in addition to the unchanged drug. The main metabolic pathways involved are dealkylation in the dimethyl amide moiety to give desmethyl- and didesmethylloperamide, both of which were in turn monohydroxylated either in the alpha-phenyl ring or possibly in the alpha-carbon in the piperidine ring. It is noteworthy that metabolites hydroxylated in the piperidine ring were isolated as pyridinium derivatives, possibly due to spontaneous aromatization of its 2,4-dihydroxy-4-(p-chlorophenyl)piperidine ring. In urine, only two metabolites were found and identified to be desmethyl- and didesmethylloperamide, since [14C]loperamide was excreted into urine only in a small amount.", "contents": "Metabolites of loperamide in rats. Following intraperitoneal administration to rats of [14C]loperamide, [carbonyl-14] 4-(p-chlorophenyl)-4-hydroxy-N,N-dimethyl-alpha, alpha-diphenyl-1-piperidine butyramide, metabolites in feces and urine were separated, and identified by means of mass spectrometry. In feces, six metabolites were identified in addition to the unchanged drug. The main metabolic pathways involved are dealkylation in the dimethyl amide moiety to give desmethyl- and didesmethylloperamide, both of which were in turn monohydroxylated either in the alpha-phenyl ring or possibly in the alpha-carbon in the piperidine ring. It is noteworthy that metabolites hydroxylated in the piperidine ring were isolated as pyridinium derivatives, possibly due to spontaneous aromatization of its 2,4-dihydroxy-4-(p-chlorophenyl)piperidine ring. In urine, only two metabolites were found and identified to be desmethyl- and didesmethylloperamide, since [14C]loperamide was excreted into urine only in a small amount."}
{"id": "PMID:476290", "title": "The identification of bile acid methyl esters by gas chromatography methane chemical ionization mass spectrometry.", "content": "Methane chemical ionization mass spectrometric data and gas chromatography retention parameters are reported for 31 bile acid methyl esters. The group includes both saturated and unsaturated compounds with up to three keto or hydroxyl groups. Major ions include the quasimolecular ions and fragments resulting from the loss of neutral species such as methanol, water and methane. The combination of gas-liquid chromatography retention and mass spectral data is sufficient for an unequivocal distinction among all the bile acids studied. Application of this data to the identification of bile acid metabolites is cited.", "contents": "The identification of bile acid methyl esters by gas chromatography methane chemical ionization mass spectrometry. Methane chemical ionization mass spectrometric data and gas chromatography retention parameters are reported for 31 bile acid methyl esters. The group includes both saturated and unsaturated compounds with up to three keto or hydroxyl groups. Major ions include the quasimolecular ions and fragments resulting from the loss of neutral species such as methanol, water and methane. The combination of gas-liquid chromatography retention and mass spectral data is sufficient for an unequivocal distinction among all the bile acids studied. Application of this data to the identification of bile acid metabolites is cited."}
{"id": "PMID:476300", "title": "Alloimmunization following prophylactic granulocyte transfusion.", "content": "Nineteen noninfected adults receiving initial induction chemotherapy for acute nonlymphocytic leukemia (ANLL) were randomized to receive either prophylactic granulocyte transfusion or platelet transfusion alone on an alternate-day schedule. An average of 11 granulocyte transfusions (range 3--19) were administered/patient with a mean dose of 11.5 X 10(9) granulocytes/transfusion. The groups were identical with respect to age, sex, number of days on study, granulocytopenic days, percent of days receiving systemic antibiotics, febrile days, complete remission rate, and incidence of minor infection. Significant transfusion reactions were much increased in the granulocyte transfusion group (7/10 versus 1/9 in controls) and were associated with the development of lymphocytotoxic antibodies (7/10 versus 4/9 controls), refractoriness to platelet transfusion, repeated fevers, and a pulmonary infiltrate in one patient. Alloimmunization to granulocytes occurred as early as the second week in some  patients complicating platelet support during induction and maintenance. No severe infections occurred in the granulocyte transfusion group while three fungal infections occurred in the controls. The high rate of alloimmunization suggests that histocompatibility considerations indicate that prophylactic granulocyte transfusion should not be routine therapy and should be studied only in investigational settings.", "contents": "Alloimmunization following prophylactic granulocyte transfusion. Nineteen noninfected adults receiving initial induction chemotherapy for acute nonlymphocytic leukemia (ANLL) were randomized to receive either prophylactic granulocyte transfusion or platelet transfusion alone on an alternate-day schedule. An average of 11 granulocyte transfusions (range 3--19) were administered/patient with a mean dose of 11.5 X 10(9) granulocytes/transfusion. The groups were identical with respect to age, sex, number of days on study, granulocytopenic days, percent of days receiving systemic antibiotics, febrile days, complete remission rate, and incidence of minor infection. Significant transfusion reactions were much increased in the granulocyte transfusion group (7/10 versus 1/9 in controls) and were associated with the development of lymphocytotoxic antibodies (7/10 versus 4/9 controls), refractoriness to platelet transfusion, repeated fevers, and a pulmonary infiltrate in one patient. Alloimmunization to granulocytes occurred as early as the second week in some  patients complicating platelet support during induction and maintenance. No severe infections occurred in the granulocyte transfusion group while three fungal infections occurred in the controls. The high rate of alloimmunization suggests that histocompatibility considerations indicate that prophylactic granulocyte transfusion should not be routine therapy and should be studied only in investigational settings."}
{"id": "PMID:476301", "title": "Prolonged hematopoiesis in a primate bone marrow culture system: characteristics of stem cell production and the hematopoietic microenvironment.", "content": "Maintenance of myelopoiesis and pluripotential stem cell production for prolonged periods in vitro hitherto has been limited to mouse bone marrow culture. In an effort to adapt the system for use in higher species, particularly in human and non-human primates, studies were undertaken using the prosimian species, Tupaia glis (tree shrew). In a number of experiments the duration of sustained normal hematopoiesis observed in cultures of this species, following a single inoculum of 5 X 10(6)--10(7) bone marrow cells, with or without addition of fresh allogeneic bone marrow exceeded 1 yr. Analysis of suspension cells obtained by weekly demidepopulation of such cultures revealed production of CFU-C, differentiating neutrophils, and basophils at high levels. Direct comparison with murine cultures indicated that in both species a complex series of cellular interactions takes place within an adherent environment of marrow-derived endothelial cells, macrophages, and fat-containing cells. Certain functional and ultrastructural features served to distinguish murine from Tupaia marrow cultures, and the prolonged duration of in vitro hematopoiesis in the latter species could be attributed to a regenerative capacity possessed by its adherent hematopoietic microenvironment. The availability of this primate marrow culture system should facilitate studies of hematopoiesis, viral leukemogenesis, and transplantation biology, which have more direct relevance to man than that provided by the existing murine system.", "contents": "Prolonged hematopoiesis in a primate bone marrow culture system: characteristics of stem cell production and the hematopoietic microenvironment. Maintenance of myelopoiesis and pluripotential stem cell production for prolonged periods in vitro hitherto has been limited to mouse bone marrow culture. In an effort to adapt the system for use in higher species, particularly in human and non-human primates, studies were undertaken using the prosimian species, Tupaia glis (tree shrew). In a number of experiments the duration of sustained normal hematopoiesis observed in cultures of this species, following a single inoculum of 5 X 10(6)--10(7) bone marrow cells, with or without addition of fresh allogeneic bone marrow exceeded 1 yr. Analysis of suspension cells obtained by weekly demidepopulation of such cultures revealed production of CFU-C, differentiating neutrophils, and basophils at high levels. Direct comparison with murine cultures indicated that in both species a complex series of cellular interactions takes place within an adherent environment of marrow-derived endothelial cells, macrophages, and fat-containing cells. Certain functional and ultrastructural features served to distinguish murine from Tupaia marrow cultures, and the prolonged duration of in vitro hematopoiesis in the latter species could be attributed to a regenerative capacity possessed by its adherent hematopoietic microenvironment. The availability of this primate marrow culture system should facilitate studies of hematopoiesis, viral leukemogenesis, and transplantation biology, which have more direct relevance to man than that provided by the existing murine system."}
{"id": "PMID:476304", "title": "Adhesion of normal and sickle erythrocytes to endothelial monolayer cultures.", "content": "Experiments were carried out to test the hypothesis that the differences between the surfaces of erythrocytes from normal and sickle cell patients are reflected in the degree of attachment to the capillary lining. An assay was used that measured the number of 51Cr-labeled erythrocytes (normal or sickle) attaching to a monolayer of endothelium cultured from calf aortas. Under these conditions, erythrocytes from sickle cell patients adhered better to the endothelium than did those from normal patients. The results suggested that the enhanced adhesion of the sickle cells to the endothelium may be partially responsible for the increased blockage of capillaries that produce the symptoms in sickle cell anemia.", "contents": "Adhesion of normal and sickle erythrocytes to endothelial monolayer cultures. Experiments were carried out to test the hypothesis that the differences between the surfaces of erythrocytes from normal and sickle cell patients are reflected in the degree of attachment to the capillary lining. An assay was used that measured the number of 51Cr-labeled erythrocytes (normal or sickle) attaching to a monolayer of endothelium cultured from calf aortas. Under these conditions, erythrocytes from sickle cell patients adhered better to the endothelium than did those from normal patients. The results suggested that the enhanced adhesion of the sickle cells to the endothelium may be partially responsible for the increased blockage of capillaries that produce the symptoms in sickle cell anemia."}
{"id": "PMID:476305", "title": "Serum erythropoietin concentration in chronic renal failure: relationship to degree of anemia and excretory renal function.", "content": "By use of the fetal mouse liver cell assay, serum erythropoietin (SEp) concentration was measured in 135 patients at various stages of chronic renal failure and in 59 healthy subjects. In patients with creatinine clearances (CCr) ranging from 2 to 40 ml/min/1.73 sq m, endocrine renal function was found to deteriorate in parallel to excretory renal function. The known negative correlation between SEp and hematocrit (Hct) was not apparent, probably because of the loss of renal mass accompanying progress of anemia and renal insufficiency. In contrast, in patients with minimal variation of residual excretory renal function, as in individual patients investigated repeatedly within a short period of time, changes of Hct were always accompanied by opposite changes of corresponding SEp concentrations. Thus, patients with chronic renal failure have a sustained regulatory feedback mechanism between Hct and SEp, which probably works at a lower level.", "contents": "Serum erythropoietin concentration in chronic renal failure: relationship to degree of anemia and excretory renal function. By use of the fetal mouse liver cell assay, serum erythropoietin (SEp) concentration was measured in 135 patients at various stages of chronic renal failure and in 59 healthy subjects. In patients with creatinine clearances (CCr) ranging from 2 to 40 ml/min/1.73 sq m, endocrine renal function was found to deteriorate in parallel to excretory renal function. The known negative correlation between SEp and hematocrit (Hct) was not apparent, probably because of the loss of renal mass accompanying progress of anemia and renal insufficiency. In contrast, in patients with minimal variation of residual excretory renal function, as in individual patients investigated repeatedly within a short period of time, changes of Hct were always accompanied by opposite changes of corresponding SEp concentrations. Thus, patients with chronic renal failure have a sustained regulatory feedback mechanism between Hct and SEp, which probably works at a lower level."}
{"id": "PMID:476306", "title": "Serum lactic dehydrogenase as a prognostic tool for non-Hodgkin lymphomas.", "content": "Serum LDH levels have been found to be significantly increased in non-Hodgkin lymphoma (NHL) patients, both histiocytic and lymphocytic. The duration of survival of NHL negatively correlates with the level of serum lactic dehydrogenase (LDH), and statistical analysis reveals that patients with lower levels of LDH have a longer survival rate than the patients with higher LDH activity, irrespective of their histologic classification. The analysis of the results by the Test for Trend in Prognosis allows us to establish that the correlation of the rate of survival and LDH levels is independent from other clinical parameters.", "contents": "Serum lactic dehydrogenase as a prognostic tool for non-Hodgkin lymphomas. Serum LDH levels have been found to be significantly increased in non-Hodgkin lymphoma (NHL) patients, both histiocytic and lymphocytic. The duration of survival of NHL negatively correlates with the level of serum lactic dehydrogenase (LDH), and statistical analysis reveals that patients with lower levels of LDH have a longer survival rate than the patients with higher LDH activity, irrespective of their histologic classification. The analysis of the results by the Test for Trend in Prognosis allows us to establish that the correlation of the rate of survival and LDH levels is independent from other clinical parameters."}
{"id": "PMID:476307", "title": "Megakaryocyte colony formation from human bone marrow precursors.", "content": "We report the growth in plasma clot culture of megakaryocyte colonies from adult bone marrow cells with the use of four different sources of erythropoietin (Ep) as the stimulating factor. A major proportion of the megakaryocyte colonies (75%) were pure, while the others were mixed, involving erythroblasts and megakaryocytes. Ultrastructural studies have shown that the maturation of megakaryocytes was essentially normal and that platelet shedding occurred. Megakaryocyte colony formation required a large number of plated cells (greater than 3 X 10(5)/ml). In the absence of erythropoietin, rare spontaneous megakaryocyte colonies could be observed, while no erythroid colonies were present. However, erythropoietin induced a fivefold increase in the total number of colonies. These data suggest that erythropoietin is involved in the differentiation of human megakaryocytes, but that it does not act alone, since another factor related to the number of seeded cells appears essential for the formation of human megakaryocyte colonies.", "contents": "Megakaryocyte colony formation from human bone marrow precursors. We report the growth in plasma clot culture of megakaryocyte colonies from adult bone marrow cells with the use of four different sources of erythropoietin (Ep) as the stimulating factor. A major proportion of the megakaryocyte colonies (75%) were pure, while the others were mixed, involving erythroblasts and megakaryocytes. Ultrastructural studies have shown that the maturation of megakaryocytes was essentially normal and that platelet shedding occurred. Megakaryocyte colony formation required a large number of plated cells (greater than 3 X 10(5)/ml). In the absence of erythropoietin, rare spontaneous megakaryocyte colonies could be observed, while no erythroid colonies were present. However, erythropoietin induced a fivefold increase in the total number of colonies. These data suggest that erythropoietin is involved in the differentiation of human megakaryocytes, but that it does not act alone, since another factor related to the number of seeded cells appears essential for the formation of human megakaryocyte colonies."}
{"id": "PMID:476308", "title": "Dense (aged) circulating red cells contain normal concentrations of adenosine triphosphate (ATP).", "content": "The densest 0.1%--1% of circulating red cells were separated from fresh blood, and the ATP content of a representative sample of such cells was determined. The dense (\"old\") cells had decreased amounts of ATP relative to unfractionated cells. However, the dense cells were also smaller, and the concentration of ATP in these cells was the same as in controls. Therefore, it seems unlikely that loss of cellular ATP is a causative factor in removal of senescent red cells from the circulation.", "contents": "Dense (aged) circulating red cells contain normal concentrations of adenosine triphosphate (ATP). The densest 0.1%--1% of circulating red cells were separated from fresh blood, and the ATP content of a representative sample of such cells was determined. The dense (\"old\") cells had decreased amounts of ATP relative to unfractionated cells. However, the dense cells were also smaller, and the concentration of ATP in these cells was the same as in controls. Therefore, it seems unlikely that loss of cellular ATP is a causative factor in removal of senescent red cells from the circulation."}
{"id": "PMID:476309", "title": "Erythroid colony stimulating and inhibiting cells in peripheral blood of transfused dogs: separation of function by velocity sedimentation.", "content": "We have previously shown that the addition of normal dog peripheral blood lymphocytes (PBL) to cultures of allogeneic marrow increases the number of marrow-derived erythroid colonies (EC), but that PBL from transfused dogs usually inhibit EC growth from marrow of the transfusion donor. In this study, the cells in normal dog PBL responsible for stimulating EC growth were shown to sediment in a narrow peak at 4.30 mm/hr. A similar population of stimulating cells exists in transfused dogs and can be separated, on the basis of size, from cells that inhibit EC growth. EC-stimulating cells from transfused dog PBL sediment at 3.3--5.0 mm/hr, while cells responsible for inhibition are larger and sediment more rapidly at 5.4--8.1 mm/hr. These data demonstrate that cells capable of stimulating allogeneic EC are present in transfused dogs, but their stimulating ability is masked by the presence of EC-inhibiting cells. Thus, coculture experiments designed to test lymphocyte/marrow cell interactions may miss significant but opposing effects if unfractionated cells are used.", "contents": "Erythroid colony stimulating and inhibiting cells in peripheral blood of transfused dogs: separation of function by velocity sedimentation. We have previously shown that the addition of normal dog peripheral blood lymphocytes (PBL) to cultures of allogeneic marrow increases the number of marrow-derived erythroid colonies (EC), but that PBL from transfused dogs usually inhibit EC growth from marrow of the transfusion donor. In this study, the cells in normal dog PBL responsible for stimulating EC growth were shown to sediment in a narrow peak at 4.30 mm/hr. A similar population of stimulating cells exists in transfused dogs and can be separated, on the basis of size, from cells that inhibit EC growth. EC-stimulating cells from transfused dog PBL sediment at 3.3--5.0 mm/hr, while cells responsible for inhibition are larger and sediment more rapidly at 5.4--8.1 mm/hr. These data demonstrate that cells capable of stimulating allogeneic EC are present in transfused dogs, but their stimulating ability is masked by the presence of EC-inhibiting cells. Thus, coculture experiments designed to test lymphocyte/marrow cell interactions may miss significant but opposing effects if unfractionated cells are used."}
{"id": "PMID:476311", "title": "Leukocyte alkaline phosphatase in malignancies.", "content": "The leukocyte alklaine phosphatase (LAP) levels were determined in 183 patients with malignant diseases and 71 normal controls. The median LAP scores were 64 units (range 0 to 290) for the patients and 55 (range 2 to 158) for the controls, respectively, and no significant difference could be established. When analyzed according to primary malignancy, only in patients with Hodgkin's disease (n = 14) was the median value higher than normal (p less than 0.001). In patients with distant metastases (n = 48), higher LAP levels were demonstrated (M = 76, range 21 to 290) as compared to patients with no evidence of metastases (M = 53, range 0 to 229), (p less than 0.01). Thus, LAP activity has very limited value in the diagnosis of malignancies. Its elevation in the presence of malignant disease might, however, indicate metastases.", "contents": "Leukocyte alkaline phosphatase in malignancies. The leukocyte alklaine phosphatase (LAP) levels were determined in 183 patients with malignant diseases and 71 normal controls. The median LAP scores were 64 units (range 0 to 290) for the patients and 55 (range 2 to 158) for the controls, respectively, and no significant difference could be established. When analyzed according to primary malignancy, only in patients with Hodgkin's disease (n = 14) was the median value higher than normal (p less than 0.001). In patients with distant metastases (n = 48), higher LAP levels were demonstrated (M = 76, range 21 to 290) as compared to patients with no evidence of metastases (M = 53, range 0 to 229), (p less than 0.01). Thus, LAP activity has very limited value in the diagnosis of malignancies. Its elevation in the presence of malignant disease might, however, indicate metastases."}
{"id": "PMID:476312", "title": "Congenital dyserythropoietic anemia type I: report of a pair of siblings in Japan.", "content": "This is the first case of two siblings with congenital dyserythrobpoietic anemia (CDA) type I to be reported from a Japanese family. Both of these cases showed characteristic morphological aberration of erythroid precursors, ineffective erythropoiesis, and negative acidified serum test. The ultrastructural study of erythroblasts also revealed characteristics quite compatible with CDA type I.", "contents": "Congenital dyserythropoietic anemia type I: report of a pair of siblings in Japan. This is the first case of two siblings with congenital dyserythrobpoietic anemia (CDA) type I to be reported from a Japanese family. Both of these cases showed characteristic morphological aberration of erythroid precursors, ineffective erythropoiesis, and negative acidified serum test. The ultrastructural study of erythroblasts also revealed characteristics quite compatible with CDA type I."}
{"id": "PMID:476313", "title": "Erythrocyte agglomeration and survival studies in citrate-phosphate-dextrose (CPD) units.", "content": "We have previously reported increased tendency to agglomeration in CPD-stored erythrocytes. In the present study we investigated the deterioration pattern of fresh units, as detected by agglomeration, free hemoglobin levels, ATP levels, osmotic fragility, and certain enzymatic activities. When the negative test was converted to a positive one, the 24-h survival values of these units were determined by autotransfusion. A positive agglomeration occurred while viability was still above 70%. Fresh units had high ATP levels and negative agglomeration while old units had low ATP levels and positive agglomeration. However, in intermediate-storage units, no definite correlation between ATP and agglomeration was found. Agglomeration seems to reflect the degree of changes in stored erythrocytes. However, further studies are needed before this test may serve for prediction of post-transfusion viability.", "contents": "Erythrocyte agglomeration and survival studies in citrate-phosphate-dextrose (CPD) units. We have previously reported increased tendency to agglomeration in CPD-stored erythrocytes. In the present study we investigated the deterioration pattern of fresh units, as detected by agglomeration, free hemoglobin levels, ATP levels, osmotic fragility, and certain enzymatic activities. When the negative test was converted to a positive one, the 24-h survival values of these units were determined by autotransfusion. A positive agglomeration occurred while viability was still above 70%. Fresh units had high ATP levels and negative agglomeration while old units had low ATP levels and positive agglomeration. However, in intermediate-storage units, no definite correlation between ATP and agglomeration was found. Agglomeration seems to reflect the degree of changes in stored erythrocytes. However, further studies are needed before this test may serve for prediction of post-transfusion viability."}
{"id": "PMID:476314", "title": "Human cold agglutinins against \"cryptic\" erythrocyte antigens.", "content": "Two examples of human IgM cold agglutinins agglutinated human RBC only after enzyme treatment in vitro. Proteases were optimally effective, neuraminidase was also effective. The cold agglutinins did not coat native RBC but were directed against \"cryptic\" RBC determinants. The cold agglutinins belonged to the anti -I/-i complex indicating a \"new\" type of I/i determinants. They were strongly accessible to cold agglutinin interaction on native RBC of a patient with congenital dyserythropoietic anaemia. Enzyme treatment of RBC was shown to be not only suited for defining cold agglutinin specificities but also essential for detecting the \"new\" type of cold agglutinins, obviously causing autoimmune haemolytic anaemia in vivo.", "contents": "Human cold agglutinins against \"cryptic\" erythrocyte antigens. Two examples of human IgM cold agglutinins agglutinated human RBC only after enzyme treatment in vitro. Proteases were optimally effective, neuraminidase was also effective. The cold agglutinins did not coat native RBC but were directed against \"cryptic\" RBC determinants. The cold agglutinins belonged to the anti -I/-i complex indicating a \"new\" type of I/i determinants. They were strongly accessible to cold agglutinin interaction on native RBC of a patient with congenital dyserythropoietic anaemia. Enzyme treatment of RBC was shown to be not only suited for defining cold agglutinin specificities but also essential for detecting the \"new\" type of cold agglutinins, obviously causing autoimmune haemolytic anaemia in vivo."}
{"id": "PMID:476315", "title": "A haemagglutination inhibition test kit for routine analysis of erythropoietin.", "content": "A commercially available haemagglutination inhibition test kit for routine analysis of erythropoietin was shown to be unable to detect elevated levels of serum Epo.", "contents": "A haemagglutination inhibition test kit for routine analysis of erythropoietin. A commercially available haemagglutination inhibition test kit for routine analysis of erythropoietin was shown to be unable to detect elevated levels of serum Epo."}
{"id": "PMID:476316", "title": "Results-oriented management though MBO.", "content": "Management by Objectives (MBO) as it has been implemented in the Houston Academy of Medicine--Texas Medical Center Library is described. That MBO must be a total management system and not just another library program is emphasized throughout the discussion and definitions of the MBO system parts: (1) mission statement; (2) role functions; (3) role relationships; (4) effectiveness areas; (5) objective; (6) action plans; and (7) performance review and evaluation. Examples from the library's implementation are given within the discussion of each part to give the reader a clearer picture of the library's actual experiences with the MBO process. Tables are included for further clarification. In conclusion some points are made which the author feels are particularly crucial to any library MBO implementation.", "contents": "Results-oriented management though MBO. Management by Objectives (MBO) as it has been implemented in the Houston Academy of Medicine--Texas Medical Center Library is described. That MBO must be a total management system and not just another library program is emphasized throughout the discussion and definitions of the MBO system parts: (1) mission statement; (2) role functions; (3) role relationships; (4) effectiveness areas; (5) objective; (6) action plans; and (7) performance review and evaluation. Examples from the library's implementation are given within the discussion of each part to give the reader a clearer picture of the library's actual experiences with the MBO process. Tables are included for further clarification. In conclusion some points are made which the author feels are particularly crucial to any library MBO implementation."}
{"id": "PMID:476317", "title": "A regional cooperative acquisition program for monographs.", "content": "A cooperative acquisition program for monographs for the twelve resource libraries in Region IX of the Regional Medical Library Network is described. Each of the participating libraries has agreed to purchase all books of an assigned publisher which fall within a prescribed subject-format profile. It is hoped that this will help to reduce unnecessary duplication and contribute toward the development of resources in the region.", "contents": "A regional cooperative acquisition program for monographs. A cooperative acquisition program for monographs for the twelve resource libraries in Region IX of the Regional Medical Library Network is described. Each of the participating libraries has agreed to purchase all books of an assigned publisher which fall within a prescribed subject-format profile. It is hoped that this will help to reduce unnecessary duplication and contribute toward the development of resources in the region."}
{"id": "PMID:476318", "title": "Medical school graduates' retrospective evaluation of a clinical medical librarian program.", "content": "This paper reports on the results of a survey of sixty-six graduates of the University of Missouri-Kansas City (UMKC) School of Medicine conducted in the spring of 1977. The graduates were questioned about their present library use behavior and their restrospective perceptions of the clinical medical librarian (CML) services which they received as medical students at UMKC. The results show that these young physicians, after regular association with other, more tradional medical library services, hold very positive impressions of the CML program. The graduates also typically credit the CML'S with helping them to learn to use library resources effectively. These retrospective perceptions of the CML match the short-term benefits reported in other studies of similar programs.", "contents": "Medical school graduates' retrospective evaluation of a clinical medical librarian program. This paper reports on the results of a survey of sixty-six graduates of the University of Missouri-Kansas City (UMKC) School of Medicine conducted in the spring of 1977. The graduates were questioned about their present library use behavior and their restrospective perceptions of the clinical medical librarian (CML) services which they received as medical students at UMKC. The results show that these young physicians, after regular association with other, more tradional medical library services, hold very positive impressions of the CML program. The graduates also typically credit the CML'S with helping them to learn to use library resources effectively. These retrospective perceptions of the CML match the short-term benefits reported in other studies of similar programs."}
{"id": "PMID:476319", "title": "New library buildings: the Health Sciences Library, Memorial University of Newfoundland, St. John's.", "content": "The new Health Sciences Library of Memorial University of Newfoundland is described and illustrated. A library facility that forms part of a larger health sciences center, this is a medium-sized academic health sciences library built on a single level. Along with a physical description of the library and its features, the concepts of single-level libraries, phased occupancy, and the project management approach to building a large health center library are discussed in detail.", "contents": "New library buildings: the Health Sciences Library, Memorial University of Newfoundland, St. John's. The new Health Sciences Library of Memorial University of Newfoundland is described and illustrated. A library facility that forms part of a larger health sciences center, this is a medium-sized academic health sciences library built on a single level. Along with a physical description of the library and its features, the concepts of single-level libraries, phased occupancy, and the project management approach to building a large health center library are discussed in detail."}
{"id": "PMID:476320", "title": "Cooperation strengthens small hospital libraries in a rural area of New England: a five-year experience.", "content": "Before 1970, library facilities and services at the small hospitals in rural Vermont were essentially nonexistent. Similar findings were later encountered along the Connecticut River in New Hampshire and in a small area of upstate New York. The Hospital Library Development Services program was established at the University of Vermont's Dana Medical Library to improve these conditions. Financial assistance was received from the National Library of Medicine, and by the end of 1974, thirty-three hospitals had staffed libraries. Earlier that year it has been decided to begin emphasing cooperation among the developing libraries, including the production of union lists and regular meetings of staff members from geographically proximate hospital libaries to plan and implement various activities. An additional one-year award from NLM was received in 1975. Results achieved during and after the period of grant support are reported. Cooperation among hospital libraries is seen as a feasible and beneficial undertaking provided that the participating libraries are internally supported and developing.", "contents": "Cooperation strengthens small hospital libraries in a rural area of New England: a five-year experience. Before 1970, library facilities and services at the small hospitals in rural Vermont were essentially nonexistent. Similar findings were later encountered along the Connecticut River in New Hampshire and in a small area of upstate New York. The Hospital Library Development Services program was established at the University of Vermont's Dana Medical Library to improve these conditions. Financial assistance was received from the National Library of Medicine, and by the end of 1974, thirty-three hospitals had staffed libraries. Earlier that year it has been decided to begin emphasing cooperation among the developing libraries, including the production of union lists and regular meetings of staff members from geographically proximate hospital libaries to plan and implement various activities. An additional one-year award from NLM was received in 1975. Results achieved during and after the period of grant support are reported. Cooperation among hospital libraries is seen as a feasible and beneficial undertaking provided that the participating libraries are internally supported and developing."}
{"id": "PMID:476347", "title": "Male infertility.", "content": "The modern management of male subfertility is based on recognition and correction of the underlying defect. Treatment starts nonspecifically with improvement in the general environment of spermatogenesis and any surgical abnormalities are dealt with. If supplementary endocrine treatment is necessary it is given rationally after estimation of pituitary and testicular hormone levels. The presence or absence of antisperm antibodies is established and their effect on sperm behaviour is observed before and during treatment. Progress in understanding the complex processes involved in human reproduction requires careful observation of accurately defined facts. Much research remains to be done.", "contents": "Male infertility. The modern management of male subfertility is based on recognition and correction of the underlying defect. Treatment starts nonspecifically with improvement in the general environment of spermatogenesis and any surgical abnormalities are dealt with. If supplementary endocrine treatment is necessary it is given rationally after estimation of pituitary and testicular hormone levels. The presence or absence of antisperm antibodies is established and their effect on sperm behaviour is observed before and during treatment. Progress in understanding the complex processes involved in human reproduction requires careful observation of accurately defined facts. Much research remains to be done."}
{"id": "PMID:476349", "title": "Ambulatory ECG monitoring.", "content": "The development and widespread application of 24-hour ambulatory ECG monitoring in the last 15 years represent major advances in cardiology. It is the most sensitive method of detecting arrhythmias and allows objective evaluation of such abnormalities and their response to treatment as the patient continues the activities of daily life. Information on prognosis and early diagnosis in patients with coronary artery disease has been obtained but more data and a greater understanding of their relevance in normal populations are necessary. Until then the significance of the findings in many situations, especially in asymptomatic patients, will remain speculative and decisions on whether or not to institute therapy will continue to be somewhat arbitrary.", "contents": "Ambulatory ECG monitoring. The development and widespread application of 24-hour ambulatory ECG monitoring in the last 15 years represent major advances in cardiology. It is the most sensitive method of detecting arrhythmias and allows objective evaluation of such abnormalities and their response to treatment as the patient continues the activities of daily life. Information on prognosis and early diagnosis in patients with coronary artery disease has been obtained but more data and a greater understanding of their relevance in normal populations are necessary. Until then the significance of the findings in many situations, especially in asymptomatic patients, will remain speculative and decisions on whether or not to institute therapy will continue to be somewhat arbitrary."}
{"id": "PMID:476356", "title": "High-dose propranolol in schizophrenia.", "content": "Eight male schizophrenics were treated in an open study with d.l. propranolol. The dose was increased by 160 mg/day until a maximum of 2,400 mg/day was achieved on day 15, and this remained constant until day 21. Seven patients showed significant clinical evidence of psychiatric improvement, while the incidence of toxic side-effects was low. There was wide inter-patient variation in plasma levels of propranolol. Despite a significant increase of plasma propranolol between day 15 and day 21 of the study, there was no significant change in serum prolactin.", "contents": "High-dose propranolol in schizophrenia. Eight male schizophrenics were treated in an open study with d.l. propranolol. The dose was increased by 160 mg/day until a maximum of 2,400 mg/day was achieved on day 15, and this remained constant until day 21. Seven patients showed significant clinical evidence of psychiatric improvement, while the incidence of toxic side-effects was low. There was wide inter-patient variation in plasma levels of propranolol. Despite a significant increase of plasma propranolol between day 15 and day 21 of the study, there was no significant change in serum prolactin."}
{"id": "PMID:476354", "title": "Motivation, responsibility and sickness in the psychiatric treatment of alcoholism.", "content": "Two dominant themes of alcohological thinking and practice are considered in this paper; the disease concept and the concept of motivation. Both are examined in terms of (a) the rights, obligations and responsibilities associated with different types of illness and degrees of severity, and (b) the routine care and treatment of alcoholism in two out-patient alcoholism clinics. The allocation of responsibility and attribution of motivation in psychiatric alcoholism consultations are examined. Finally, the relationship between responsibility allocation, motivation attribution and judgements of a potentially moral nature is considered. It is concluded that a Mutual Participation model of sickness behaviour and doctor-patient relationship is operative in the psychiatric treatment of alcoholism, based upon patients' self-responsibility and strength of character (will-power). In this respect, the psychiatric treatment of alcoholism contrasts with other (particularly non-psychiatric) conditions and treatments.", "contents": "Motivation, responsibility and sickness in the psychiatric treatment of alcoholism. Two dominant themes of alcohological thinking and practice are considered in this paper; the disease concept and the concept of motivation. Both are examined in terms of (a) the rights, obligations and responsibilities associated with different types of illness and degrees of severity, and (b) the routine care and treatment of alcoholism in two out-patient alcoholism clinics. The allocation of responsibility and attribution of motivation in psychiatric alcoholism consultations are examined. Finally, the relationship between responsibility allocation, motivation attribution and judgements of a potentially moral nature is considered. It is concluded that a Mutual Participation model of sickness behaviour and doctor-patient relationship is operative in the psychiatric treatment of alcoholism, based upon patients' self-responsibility and strength of character (will-power). In this respect, the psychiatric treatment of alcoholism contrasts with other (particularly non-psychiatric) conditions and treatments."}
{"id": "PMID:476357", "title": "Averaged evoked responses in relation to cognitive and affective state of elderly psychiatric patients.", "content": "Averaged cortical evoked responses to auditory and somatosensory stimuli were recorded in elderly depressives, dements, patients with a combination of depression and dementia and normal elderly controls. The subjects were also given a battery of cognitive tests and clinical ratings at stated intervals. The latency of the auditory response was significantly longer in dements than in controls. Depressives had intermediate latencies which did not return to normal after recovery. Although somatosensory stimulu produced the results that pointed in the same direction, the mean response latencies were not statistically significant. Mixed cases were too few for separate statistical analysis but their latencies fell between those of the dements and those of the depressives. Significant correlations emerged between latencies of auditory responses and some cognitive tests, but not with measures of depressive symptomatology. The delay in response to auditory stimuli may be a useful adjunct to diagnosis: in depressives it may reflect organic cerebral change playing a part in the emergence of depressive symptoms in old age.", "contents": "Averaged evoked responses in relation to cognitive and affective state of elderly psychiatric patients. Averaged cortical evoked responses to auditory and somatosensory stimuli were recorded in elderly depressives, dements, patients with a combination of depression and dementia and normal elderly controls. The subjects were also given a battery of cognitive tests and clinical ratings at stated intervals. The latency of the auditory response was significantly longer in dements than in controls. Depressives had intermediate latencies which did not return to normal after recovery. Although somatosensory stimulu produced the results that pointed in the same direction, the mean response latencies were not statistically significant. Mixed cases were too few for separate statistical analysis but their latencies fell between those of the dements and those of the depressives. Significant correlations emerged between latencies of auditory responses and some cognitive tests, but not with measures of depressive symptomatology. The delay in response to auditory stimuli may be a useful adjunct to diagnosis: in depressives it may reflect organic cerebral change playing a part in the emergence of depressive symptoms in old age."}
{"id": "PMID:476358", "title": "Reality orientation and staff attention: a controlled study.", "content": "A trial was carried out of Reality Orientation (R.O.) in a residential home for the elderly mentally infirm with a 'social therapy' control group, receiving the same amount of staff attention as the R.O. group, in addition to an untreated control group. R.O. residents improved more on tests of information and orientation and various aspects of memory than controls. These results support the hypothesis that the nature of staff attention is crucial and that it should encourage and reward appropriate behaviour. No differential effects of treatment were found on rating scale assessments of the resident's functioning in the home.", "contents": "Reality orientation and staff attention: a controlled study. A trial was carried out of Reality Orientation (R.O.) in a residential home for the elderly mentally infirm with a 'social therapy' control group, receiving the same amount of staff attention as the R.O. group, in addition to an untreated control group. R.O. residents improved more on tests of information and orientation and various aspects of memory than controls. These results support the hypothesis that the nature of staff attention is crucial and that it should encourage and reward appropriate behaviour. No differential effects of treatment were found on rating scale assessments of the resident's functioning in the home."}
{"id": "PMID:476360", "title": "Problems of repertory grid analysis and a cluster analysis solution.", "content": "Statistical and requirement difficulties encountered with existing methods of Repertory Grid Analysis are considered. A simple purpose-built method of Cluster Analysis is outlined, and its extension to the difficult problem of comparative analysis described.", "contents": "Problems of repertory grid analysis and a cluster analysis solution. Statistical and requirement difficulties encountered with existing methods of Repertory Grid Analysis are considered. A simple purpose-built method of Cluster Analysis is outlined, and its extension to the difficult problem of comparative analysis described."}
{"id": "PMID:476355", "title": "Alcohol withdrawal states and the need for treatment.", "content": "In an attempt to evaluate the clinical significance of alcohol withdrawal, the rate of symptoms and incidence of treatment were determined in 84 female alcoholics following abrupt withdrawal. Results indicated that abstinence symptoms of an emergency type (delirium tremens, convulsions) only rarely occur and require active treatment, whereas those physical and emotional disturbances frequently seen in alcohol withdrawal need very little medical help. This finding is in contradistinction to current clinical opinion.", "contents": "Alcohol withdrawal states and the need for treatment. In an attempt to evaluate the clinical significance of alcohol withdrawal, the rate of symptoms and incidence of treatment were determined in 84 female alcoholics following abrupt withdrawal. Results indicated that abstinence symptoms of an emergency type (delirium tremens, convulsions) only rarely occur and require active treatment, whereas those physical and emotional disturbances frequently seen in alcohol withdrawal need very little medical help. This finding is in contradistinction to current clinical opinion."}
{"id": "PMID:476359", "title": "Validity and uses of a screening questionnaire (GHQ) in the community.", "content": "The 30-item General Health Questionnaire (GHQ) has been validated against the criterion of a standardized psychiatric interview in the community. Used for screening, the GHQ misclassified 25 per cent of 105 respondents, being worse for men. Specificity and sensitivity were above .70. The alternative use of the GHQ as an estimator and as an indicator of morbidity in epidemiological surveys is described and discussed.", "contents": "Validity and uses of a screening questionnaire (GHQ) in the community. The 30-item General Health Questionnaire (GHQ) has been validated against the criterion of a standardized psychiatric interview in the community. Used for screening, the GHQ misclassified 25 per cent of 105 respondents, being worse for men. Specificity and sensitivity were above .70. The alternative use of the GHQ as an estimator and as an indicator of morbidity in epidemiological surveys is described and discussed."}
{"id": "PMID:476366", "title": "Women who kill their children.", "content": "During a 6 year period (1970-75) 89 women charged with the killing or attempted murder of their children were examined in a female remand prison. Six types of maternal filicide were distinguished: battering mothers (36 cases), mentally ill mothers (24 cases), neonaticides (11 cases), retaliating mothers (9 cases), women who killed unwanted children (8 cases) and mercy killing (1 case). Types of filicide were compared on a number of social and psychiatric characteristics and on their offence patterns and court disposals. The operation of the Infanticide Act is discussed in the light of these findings.", "contents": "Women who kill their children. During a 6 year period (1970-75) 89 women charged with the killing or attempted murder of their children were examined in a female remand prison. Six types of maternal filicide were distinguished: battering mothers (36 cases), mentally ill mothers (24 cases), neonaticides (11 cases), retaliating mothers (9 cases), women who killed unwanted children (8 cases) and mercy killing (1 case). Types of filicide were compared on a number of social and psychiatric characteristics and on their offence patterns and court disposals. The operation of the Infanticide Act is discussed in the light of these findings."}
{"id": "PMID:476367", "title": "Depressive classification and prediction of response to phenelzine.", "content": "A variety of depressive classifications were used to predict response to four weeks' treatment with phenelzine. Better response was found in outpatients rather than inpatients, in atypical depressives, in less severe depressives with a pattern of anxiety and other neurotic symptoms, and in groups characterized as hostile and agitated. The findings, although a little patchy, gave clear support to the concept of a specific clinical group responsive to MAO inhibitors.", "contents": "Depressive classification and prediction of response to phenelzine. A variety of depressive classifications were used to predict response to four weeks' treatment with phenelzine. Better response was found in outpatients rather than inpatients, in atypical depressives, in less severe depressives with a pattern of anxiety and other neurotic symptoms, and in groups characterized as hostile and agitated. The findings, although a little patchy, gave clear support to the concept of a specific clinical group responsive to MAO inhibitors."}
{"id": "PMID:476368", "title": "Pre-anaesthetic assessment and the prevention of post-ECT morbidity.", "content": "The introduction of anaesthesia and muscle relaxation has made ECT relatively safe. In a retrospective study of the case notes of 367 consecutive patients receiving ECT over a two year period, chest X-ray and full blood count added no significant information and were ineffective in predicting morbidity, in contrast to physical examination alone.", "contents": "Pre-anaesthetic assessment and the prevention of post-ECT morbidity. The introduction of anaesthesia and muscle relaxation has made ECT relatively safe. In a retrospective study of the case notes of 367 consecutive patients receiving ECT over a two year period, chest X-ray and full blood count added no significant information and were ineffective in predicting morbidity, in contrast to physical examination alone."}
{"id": "PMID:476369", "title": "The course and determinants of reactions to myocardial infarction.", "content": "The growth of speculative theories about response to physical illness has not been backed by quantitative information. The introduction of standardized measures of psychological and social adjustment has allowed description and analysis of disability for 100 patients and their spouses two and twelve months after a first myocardial infarction. (1) Global measures of social outcome were found to be inadequate and misleading and therefore work, leisure, marriage and family relationships, sex and compliance were separately examined. (2) There was considerable continuity in individual reactions throughout convalescence and it was possible (3) to identify factors in the premorbid psychosocial state predictive of outcome and (4) to describe characteristic patterns of coping. (5) Outcome for spouses can be similarly described and understood. The findings have clinical applications for diagnosis, prophylaxis, treatment and the evaluation of medical care. The conclusion also extend our understanding of the basic principles underlying response to physical illness.", "contents": "The course and determinants of reactions to myocardial infarction. The growth of speculative theories about response to physical illness has not been backed by quantitative information. The introduction of standardized measures of psychological and social adjustment has allowed description and analysis of disability for 100 patients and their spouses two and twelve months after a first myocardial infarction. (1) Global measures of social outcome were found to be inadequate and misleading and therefore work, leisure, marriage and family relationships, sex and compliance were separately examined. (2) There was considerable continuity in individual reactions throughout convalescence and it was possible (3) to identify factors in the premorbid psychosocial state predictive of outcome and (4) to describe characteristic patterns of coping. (5) Outcome for spouses can be similarly described and understood. The findings have clinical applications for diagnosis, prophylaxis, treatment and the evaluation of medical care. The conclusion also extend our understanding of the basic principles underlying response to physical illness."}
{"id": "PMID:476370", "title": "The predictability of thought disordered speech in schizophrenic patients.", "content": "Previous research has resulted in inconsistent findings regarding the predictability of schizophrenic speech samples. It was hypothesized that the predictability of schizophrenic speech varies as a function of clinically manifest thought disorder. In an experiment based on the Cloze procedure, raters were asked to predict ten passages of schizophrenic speech and eight passages of normal speech under conditions of fourth- and fifth-word deletion. Differences emerged between the samples only for the fifth-word deletion procedure. When the schizophrenic samples were grouped according to the presence of thought disorder, thought-disordered speech was significantly less predictable than normal and non-thought disordered schizophrenic speech. Furthermore, non-thought-disordered schizophrenic speech was no less predictable than normal speech. It is concluded that schizophrenia should be more carefully defined and that thought disorder should be routinely assessed in future investigations.", "contents": "The predictability of thought disordered speech in schizophrenic patients. Previous research has resulted in inconsistent findings regarding the predictability of schizophrenic speech samples. It was hypothesized that the predictability of schizophrenic speech varies as a function of clinically manifest thought disorder. In an experiment based on the Cloze procedure, raters were asked to predict ten passages of schizophrenic speech and eight passages of normal speech under conditions of fourth- and fifth-word deletion. Differences emerged between the samples only for the fifth-word deletion procedure. When the schizophrenic samples were grouped according to the presence of thought disorder, thought-disordered speech was significantly less predictable than normal and non-thought disordered schizophrenic speech. Furthermore, non-thought-disordered schizophrenic speech was no less predictable than normal speech. It is concluded that schizophrenia should be more carefully defined and that thought disorder should be routinely assessed in future investigations."}
{"id": "PMID:476371", "title": "Relationship between cognitive defect, affect response and community adjustment in chronic schizophrenics.", "content": "The relationship between mental status and community adjustment has been examined in 100 outpatient chronic schizophrenics. Sixty-six per cent presented at least one of the signs of Schneiderian First Rank Symptoms, which, however, failed to discriminate the diagnostic subgroups. Total adjustment correlated with total mental status and appeared to differentiate the diagnostic subclassifications of schizophrenia. From the major components of mental status, anxiety and depression appear to be significantly correlated with the functioning and stress of the individual in community adjustment while primary symptoms are only peripherally correlated with adjustment.", "contents": "Relationship between cognitive defect, affect response and community adjustment in chronic schizophrenics. The relationship between mental status and community adjustment has been examined in 100 outpatient chronic schizophrenics. Sixty-six per cent presented at least one of the signs of Schneiderian First Rank Symptoms, which, however, failed to discriminate the diagnostic subgroups. Total adjustment correlated with total mental status and appeared to differentiate the diagnostic subclassifications of schizophrenia. From the major components of mental status, anxiety and depression appear to be significantly correlated with the functioning and stress of the individual in community adjustment while primary symptoms are only peripherally correlated with adjustment."}
{"id": "PMID:476373", "title": "An extension of the Random Error Coefficient of Agreement to N x N tables.", "content": "Maxwell has proposed the Random Error Coefficient of Agreement (RE), as a measure of agreement for the binary case based upon the assumption of random distribution of questionable cases. The present paper presents a more general formula for RE's computation and shows how the new form is applicable to cases with more than two response categories.", "contents": "An extension of the Random Error Coefficient of Agreement to N x N tables. Maxwell has proposed the Random Error Coefficient of Agreement (RE), as a measure of agreement for the binary case based upon the assumption of random distribution of questionable cases. The present paper presents a more general formula for RE's computation and shows how the new form is applicable to cases with more than two response categories."}
{"id": "PMID:476372", "title": "Severity of psychiatric disorder and the 30-item general health questionnaire.", "content": "The 30-item General Health Questionnaire misclassified 26 per cent of respondents in two samples of women who were interviewed by a psychiatrist using the Present State Examination. False negatives were likely to be women with chronic disorders, particularly anxiety states. False positives were likely to be distressed by severe physical illness, a recent adverse life event, or loneliness. Applying a higher threshold score to their GHQ responses would help to separate those with a diagnosable psychiatric disorder from those in states of distress.", "contents": "Severity of psychiatric disorder and the 30-item general health questionnaire. The 30-item General Health Questionnaire misclassified 26 per cent of respondents in two samples of women who were interviewed by a psychiatrist using the Present State Examination. False negatives were likely to be women with chronic disorders, particularly anxiety states. False positives were likely to be distressed by severe physical illness, a recent adverse life event, or loneliness. Applying a higher threshold score to their GHQ responses would help to separate those with a diagnosable psychiatric disorder from those in states of distress."}
{"id": "PMID:476374", "title": "Chlormethiazole abuse.", "content": "This report describes 17 cases of chlormethiazole abuse or dependence. These include one case with symptoms and signs of withdrawal and two other similar cases where undoubted dependence was combined with excessive alcohol intake. Seven other patients with alcoholism who indulged in drug-seeking behaviour involving chlormethiazole are also reported, together with a further seven abusers of various other drug who were also discovered to be taking chlormethiazole.", "contents": "Chlormethiazole abuse. This report describes 17 cases of chlormethiazole abuse or dependence. These include one case with symptoms and signs of withdrawal and two other similar cases where undoubted dependence was combined with excessive alcohol intake. Seven other patients with alcoholism who indulged in drug-seeking behaviour involving chlormethiazole are also reported, together with a further seven abusers of various other drug who were also discovered to be taking chlormethiazole."}
{"id": "PMID:476375", "title": "Consanguinity and schizophrenia in Sudan.", "content": "The rate of first-cousin marriages among the patients of schizophrenics was compared with a control group in an isolated highly inbred sudanese community, no significant difference being found.", "contents": "Consanguinity and schizophrenia in Sudan. The rate of first-cousin marriages among the patients of schizophrenics was compared with a control group in an isolated highly inbred sudanese community, no significant difference being found."}
{"id": "PMID:476385", "title": "Bilateral extracranial internal carotid artery injury due to non-penetrating trauma--report of three cases.", "content": "Angiographic features of three patients with bilateral internal carotid artery injury (spasm/dissection/thrombosis) due to non-penetrating trauma are reported. All three patients were involved in motor vehicle accidents. Focal-localizing neurological signs were present and the patients were suspected of having extracerebral haematomas. The diagnosis of traumatic spasm/dissection/thrombosis of the cervical portion of the internal carotid arteries was made following angiography. The need to include views of the cervical carotid arteries in cases of traumatic hemiparesis is stressed and possible effect of cranial CT scanning in these patients on diagnosis of carotid artery injury discussed.", "contents": "Bilateral extracranial internal carotid artery injury due to non-penetrating trauma--report of three cases. Angiographic features of three patients with bilateral internal carotid artery injury (spasm/dissection/thrombosis) due to non-penetrating trauma are reported. All three patients were involved in motor vehicle accidents. Focal-localizing neurological signs were present and the patients were suspected of having extracerebral haematomas. The diagnosis of traumatic spasm/dissection/thrombosis of the cervical portion of the internal carotid arteries was made following angiography. The need to include views of the cervical carotid arteries in cases of traumatic hemiparesis is stressed and possible effect of cranial CT scanning in these patients on diagnosis of carotid artery injury discussed."}
{"id": "PMID:476386", "title": "The value of a tracer dose in predicting the kinetics of therapeutic doses of 131I in thyrotoxicosis.", "content": "The kinetics of tracer doses of 131I, assessed by measuring uptake and effective half-life, correlate well with the kinetics of therapy doses. The effective half-life was measured using thermoluminescent discs fixed to the patients' anterior neck surface. This method gives results comparable with those obtained using an uptake probe, and met with a good response by the patients. Using the kinetics of the tracer dose to assist prescription of radioiodine therapy would considerably reduce the spread of absorbed doses to the glands of a group of patients. The case for attempting more exact dosimetry in this field is reviewed.", "contents": "The value of a tracer dose in predicting the kinetics of therapeutic doses of 131I in thyrotoxicosis. The kinetics of tracer doses of 131I, assessed by measuring uptake and effective half-life, correlate well with the kinetics of therapy doses. The effective half-life was measured using thermoluminescent discs fixed to the patients' anterior neck surface. This method gives results comparable with those obtained using an uptake probe, and met with a good response by the patients. Using the kinetics of the tracer dose to assist prescription of radioiodine therapy would considerably reduce the spread of absorbed doses to the glands of a group of patients. The case for attempting more exact dosimetry in this field is reviewed."}
{"id": "PMID:476387", "title": "Doses to patients from pantomographic and conventional dental radiography.", "content": "The increasing popularity of pantographic dental radiography and particularly its recent use for routine screening of asymptomatic patients have prompted the NRPB to compare the risks to patients from this technique with those from more conventional dental diagnostic procedures. Pantographic equipment from seven manufacturers have been investigated along with five procedures for obtaining similar information using conventional dental X-ray sets. Lithium borate thermoluminescent dosimeters located at 141 sites in the head and neck of a Rando phantom have been used to measure the mean absorbed dose to organs of interest as well as the total energy imparted to the phantom. Relative values of the energy imparted by the various techniques provide an estimate of their relative somatic risk to the patient. Pantomographic techniques were generally found to impart less than half the energy to the phantom than that given by a conventional full-mouth periapical series conducted at 45 kV, and a similar amount of energy to four bitewing intra-oral films. The restriction of the frequency of pantomographic examinations to no more than once per year during adolescence and once per three to five years during adulthood has been shown to involve only a minimal increase in the risk of somatic injury to the patient.", "contents": "Doses to patients from pantomographic and conventional dental radiography. The increasing popularity of pantographic dental radiography and particularly its recent use for routine screening of asymptomatic patients have prompted the NRPB to compare the risks to patients from this technique with those from more conventional dental diagnostic procedures. Pantographic equipment from seven manufacturers have been investigated along with five procedures for obtaining similar information using conventional dental X-ray sets. Lithium borate thermoluminescent dosimeters located at 141 sites in the head and neck of a Rando phantom have been used to measure the mean absorbed dose to organs of interest as well as the total energy imparted to the phantom. Relative values of the energy imparted by the various techniques provide an estimate of their relative somatic risk to the patient. Pantomographic techniques were generally found to impart less than half the energy to the phantom than that given by a conventional full-mouth periapical series conducted at 45 kV, and a similar amount of energy to four bitewing intra-oral films. The restriction of the frequency of pantomographic examinations to no more than once per year during adolescence and once per three to five years during adulthood has been shown to involve only a minimal increase in the risk of somatic injury to the patient."}
{"id": "PMID:476388", "title": "Radiation dose to the fetus in obstetric radiography.", "content": "The mean dose to the fetus and fetal head, together with the doses to the fetal gonads and maternal gonads, have been estimated to be 107, 56, 131 and 136 mrad respectively for an obstetric prone oblique examination of the abdomen, using a rare-earth screen-film combination. The doses were derived by measuring skin doses on a series of women in late pregnancy undergoing the above examination and by using measured depth-dose information. The position of the fetus relative to the maternal skin surface was determined by ultrasonic scanning.", "contents": "Radiation dose to the fetus in obstetric radiography. The mean dose to the fetus and fetal head, together with the doses to the fetal gonads and maternal gonads, have been estimated to be 107, 56, 131 and 136 mrad respectively for an obstetric prone oblique examination of the abdomen, using a rare-earth screen-film combination. The doses were derived by measuring skin doses on a series of women in late pregnancy undergoing the above examination and by using measured depth-dose information. The position of the fetus relative to the maternal skin surface was determined by ultrasonic scanning."}
{"id": "PMID:476389", "title": "Lung damage in C57B1 mice following thoracic irradiation: enhancement by chemotherapy.", "content": "C57Bl mice were treated with thoracic irradiation to doses in the range 12.0 to 18.9 gray. Few deaths were observed in the period 80--160 days after irradiation (an end-point of lung damage used by other investigators) and the median survival times ranged from 200 to 310 days. CBA mice treated under identical conditions predominantly died between 80--160 days and it is therefore concluded that C57Bl mice show unusually prolonged survival following this treatment. Six chemotherapeutic agents were given to C57Bl mice together with thoracic irradiation, in most cases two weeks beforehand. Adriamycin, bleomycin and cyclophosphamide enhanced the mortality of the mice. Most agents had little effect on radiation-induced skin damage.", "contents": "Lung damage in C57B1 mice following thoracic irradiation: enhancement by chemotherapy. C57Bl mice were treated with thoracic irradiation to doses in the range 12.0 to 18.9 gray. Few deaths were observed in the period 80--160 days after irradiation (an end-point of lung damage used by other investigators) and the median survival times ranged from 200 to 310 days. CBA mice treated under identical conditions predominantly died between 80--160 days and it is therefore concluded that C57Bl mice show unusually prolonged survival following this treatment. Six chemotherapeutic agents were given to C57Bl mice together with thoracic irradiation, in most cases two weeks beforehand. Adriamycin, bleomycin and cyclophosphamide enhanced the mortality of the mice. Most agents had little effect on radiation-induced skin damage."}
{"id": "PMID:476401", "title": "Effect of general practitioners' advice against smoking.", "content": "During four weeks all 2138 cigarette smokers attending the surgeries of 28 general practitioners (GPs) in five group practices in London were allocated to one of four groups: group 1 comprised non-intervention controls; group 2 comprised questionnaire-only controls; group 3 were advised by their GP to stop smoking; and group 4 were advised to stop smoking, given a leaflet to help them, and warned that they would be followed-up. Adequate data for follow-up were obtained from 1884 patients (88%) at one month and 1567 (73%) at one year. Changes in motivation and intention to stop smoking were evident immediately after advice was given. Of the people who stopped smoking, most did so because of the advice. This was achieved by motivating more people to try to stop smoking rather than increasing the success rate among those who did try. The effect was strongest during the first month but still evident over the next three months and was enhanced by the leaflet and warning about follow-up. An additional effect over the longer term was a lower relapse rate among those who stopped, but this was not enhanced by the leaflet and warning about follow-up. The proportions who stopped smoking during the first month and were still not smoking one year later were 0.3%, 1.6%, 3.3%, and 5.1% in the four groups respectively (P <0.001).The results suggest that any GP who adopts this simple routine could expect about 25 long-term successes yearly. If all GPs in the UK participated the yield would exceed half a million ex-smokers a year. This target could not be matched by increasing the present 50 or so special withdrawal clinics to 10 000.", "contents": "Effect of general practitioners' advice against smoking. During four weeks all 2138 cigarette smokers attending the surgeries of 28 general practitioners (GPs) in five group practices in London were allocated to one of four groups: group 1 comprised non-intervention controls; group 2 comprised questionnaire-only controls; group 3 were advised by their GP to stop smoking; and group 4 were advised to stop smoking, given a leaflet to help them, and warned that they would be followed-up. Adequate data for follow-up were obtained from 1884 patients (88%) at one month and 1567 (73%) at one year. Changes in motivation and intention to stop smoking were evident immediately after advice was given. Of the people who stopped smoking, most did so because of the advice. This was achieved by motivating more people to try to stop smoking rather than increasing the success rate among those who did try. The effect was strongest during the first month but still evident over the next three months and was enhanced by the leaflet and warning about follow-up. An additional effect over the longer term was a lower relapse rate among those who stopped, but this was not enhanced by the leaflet and warning about follow-up. The proportions who stopped smoking during the first month and were still not smoking one year later were 0.3%, 1.6%, 3.3%, and 5.1% in the four groups respectively (P <0.001).The results suggest that any GP who adopts this simple routine could expect about 25 long-term successes yearly. If all GPs in the UK participated the yield would exceed half a million ex-smokers a year. This target could not be matched by increasing the present 50 or so special withdrawal clinics to 10 000."}
{"id": "PMID:476402", "title": "Association between raw milk and human Salmonella dublin infection.", "content": "Between 1971 and 1975 the mean annual incidence of human Salmonella dublin infection in California increased more than five-fold. Investigation of the increase showed an association with exposure to raw milk in 44 out of 113 cases. Of these 44 patients, 35 had used certified raw milk from a single dairy. Faecal swabs confirmed S dublin infection in the dairy herd and the milk, and so a pasteurisation order was issued. S dublin appears to be an unusually invasive and life-threatening salmonella serotype: 65% of isolations were obtained from non-faecal specimens (mainly blood cultures), 89 patients (80%) were admitted to hospital and 22 patients died. Almost three-quarters of the patients were aged 20 or over, and half had serious underlying diseases, particularly leukaemias and lymphomas. Five patients presented with infected vascular lesions that included aneurysms with abscesses and infections of previous arterial graft sites. The public's increasing desire for a \"health food\" such as raw milk should be tempered with an appreciation of its attendant risk to health.", "contents": "Association between raw milk and human Salmonella dublin infection. Between 1971 and 1975 the mean annual incidence of human Salmonella dublin infection in California increased more than five-fold. Investigation of the increase showed an association with exposure to raw milk in 44 out of 113 cases. Of these 44 patients, 35 had used certified raw milk from a single dairy. Faecal swabs confirmed S dublin infection in the dairy herd and the milk, and so a pasteurisation order was issued. S dublin appears to be an unusually invasive and life-threatening salmonella serotype: 65% of isolations were obtained from non-faecal specimens (mainly blood cultures), 89 patients (80%) were admitted to hospital and 22 patients died. Almost three-quarters of the patients were aged 20 or over, and half had serious underlying diseases, particularly leukaemias and lymphomas. Five patients presented with infected vascular lesions that included aneurysms with abscesses and infections of previous arterial graft sites. The public's increasing desire for a \"health food\" such as raw milk should be tempered with an appreciation of its attendant risk to health."}
{"id": "PMID:476405", "title": "Regional organisation of neonatal intensive care in the North-west.", "content": "From January 1976 to July 1978, 234 ill neonates were transported to the regional neonatal intensive care unit (ICU) of St Mary's Hospital, Manchester, from 26 maternity hospitals three to 120 miles (4.8-193.1 km) away. Ninety per cent were transferred from 15 hospitals within a 30-mile (48.3 km) radius of the ICU. Most referring hospitals had more than 2000 births a year and offered good nursing supervision of high-risk neonates in conventional special care baby units (SCBUs). In 200 of the 234 babies respiratory symptoms precipitated referral, hyaline membrane disease being the most common final diagnosis. Altogether 143 babies received definitive mechanical ventilation, and most of them were ventilated in the ambulance en route to the ICU. The neonatal survival rates for all babies and for those who received mechanical ventilation were 61% and 45% respectively. Lethal inoperable malformations and tentorial tears accounted for 23 of the 92 deaths.A crisis-orientated referral service directed towards the treatment of babies with impending or actual respiratory failure needs close collaboration between the regional ICU and referring SCBUs, with parallel development of their facilities and skills. A complementary but distinct pattern of referral is necessary to cater for those high-risk but not critically ill babies who are born in hospitals that lack staff and facilities to provide the vigilant and anticipatory care that is so necessary.", "contents": "Regional organisation of neonatal intensive care in the North-west. From January 1976 to July 1978, 234 ill neonates were transported to the regional neonatal intensive care unit (ICU) of St Mary's Hospital, Manchester, from 26 maternity hospitals three to 120 miles (4.8-193.1 km) away. Ninety per cent were transferred from 15 hospitals within a 30-mile (48.3 km) radius of the ICU. Most referring hospitals had more than 2000 births a year and offered good nursing supervision of high-risk neonates in conventional special care baby units (SCBUs). In 200 of the 234 babies respiratory symptoms precipitated referral, hyaline membrane disease being the most common final diagnosis. Altogether 143 babies received definitive mechanical ventilation, and most of them were ventilated in the ambulance en route to the ICU. The neonatal survival rates for all babies and for those who received mechanical ventilation were 61% and 45% respectively. Lethal inoperable malformations and tentorial tears accounted for 23 of the 92 deaths.A crisis-orientated referral service directed towards the treatment of babies with impending or actual respiratory failure needs close collaboration between the regional ICU and referring SCBUs, with parallel development of their facilities and skills. A complementary but distinct pattern of referral is necessary to cater for those high-risk but not critically ill babies who are born in hospitals that lack staff and facilities to provide the vigilant and anticipatory care that is so necessary."}
{"id": "PMID:476407", "title": "Relative costs of home and hospital confinement.", "content": "The first study in a series concerned with the costs of different strategies of maternity care showed the differences between the costs of booked confinement at home, in a GP hospital for 48 hours, and in a consultant obstetric unit for 48 hours to be small. The study costed 1977 home confinements in Dudley and compared them with matched samples of hospital births. The factors that compensated, in the home booked deliveries, for the cost of hospital stay were an average of 11 extra visits from community midwives, midwife attendance for six hours at delivery, and a proportion who were transferred to hospital after receiving the extra antenatal care of a home booked delivery. This differential transfer rate is a crucial factor that merits further investigation. Family-borne costs were included in the study. The most significant was time off work by the husband. Average family costs were 68 pounds, about 30% of public sector costs. Any acceptable strategy of home confinements must provide for last-minute transfers to hospital.", "contents": "Relative costs of home and hospital confinement. The first study in a series concerned with the costs of different strategies of maternity care showed the differences between the costs of booked confinement at home, in a GP hospital for 48 hours, and in a consultant obstetric unit for 48 hours to be small. The study costed 1977 home confinements in Dudley and compared them with matched samples of hospital births. The factors that compensated, in the home booked deliveries, for the cost of hospital stay were an average of 11 extra visits from community midwives, midwife attendance for six hours at delivery, and a proportion who were transferred to hospital after receiving the extra antenatal care of a home booked delivery. This differential transfer rate is a crucial factor that merits further investigation. Family-borne costs were included in the study. The most significant was time off work by the husband. Average family costs were 68 pounds, about 30% of public sector costs. Any acceptable strategy of home confinements must provide for last-minute transfers to hospital."}
{"id": "PMID:476433", "title": "Diagnostic value of routine exercise testing in hospital patients with angina pectoris.", "content": "A group of patients with angina pectoris were investigated prospectively using a simple and well-recognised exercise test protocol, and S-T segment displacements during exercise were correlated with the results of coronary arteriography. Definitive exercise results in 100 patients correlated well with findings on coronary angiograms: 69 out of 70 patients with positive exercise test results had significant coronary disease on angiography, while 23 out of 30 with negative exercise test results had no significant coronary disease. In six patients a period of physical training changed indeterminate initial tests to definitive ones after the training. These results show that the use of a widely available exercise test and a light physical training programme, with rigid adherence to the protocol, can reduce the overall demand on coronary arteriography.", "contents": "Diagnostic value of routine exercise testing in hospital patients with angina pectoris. A group of patients with angina pectoris were investigated prospectively using a simple and well-recognised exercise test protocol, and S-T segment displacements during exercise were correlated with the results of coronary arteriography. Definitive exercise results in 100 patients correlated well with findings on coronary angiograms: 69 out of 70 patients with positive exercise test results had significant coronary disease on angiography, while 23 out of 30 with negative exercise test results had no significant coronary disease. In six patients a period of physical training changed indeterminate initial tests to definitive ones after the training. These results show that the use of a widely available exercise test and a light physical training programme, with rigid adherence to the protocol, can reduce the overall demand on coronary arteriography."}
{"id": "PMID:476434", "title": "Serum thyroglobulin concentrations and whole-body radioiodine scan in follow-up of differentiated thyroid cancer after thyroid ablation.", "content": "Measurement of serum thyroglobulin (Tg) concentrations and whole-body radioiodine scan were performed simultaneously during follow-up of 32 patients with differentiated thyroid cancer who had undergone thyroid ablation by operation and radioiodine. Almost all patients in whom serum Tg was undetectable had normal scans. Concentrations exceeding 50 ng/ml were invariably associated with residual or metastatic tumour uptake in the scan. Out of 21 observations of detectable values below 50 ng/ml, 14 were in patients whose scans showed subclinical or sub-radiological tumour uptake and seven in patients with normal scans. The sensitivity of serum Tg as a tumour marker compared favourably to that of the whole-body scan. A scan is unnecessary when serum Tg is undetectable, but in patients with detectable serum Tg concentrations, particularly if these are below 50 ng/ml, a scan is important to assess and localise tumour uptake of iodine before advising treatmet with iodine-131.", "contents": "Serum thyroglobulin concentrations and whole-body radioiodine scan in follow-up of differentiated thyroid cancer after thyroid ablation. Measurement of serum thyroglobulin (Tg) concentrations and whole-body radioiodine scan were performed simultaneously during follow-up of 32 patients with differentiated thyroid cancer who had undergone thyroid ablation by operation and radioiodine. Almost all patients in whom serum Tg was undetectable had normal scans. Concentrations exceeding 50 ng/ml were invariably associated with residual or metastatic tumour uptake in the scan. Out of 21 observations of detectable values below 50 ng/ml, 14 were in patients whose scans showed subclinical or sub-radiological tumour uptake and seven in patients with normal scans. The sensitivity of serum Tg as a tumour marker compared favourably to that of the whole-body scan. A scan is unnecessary when serum Tg is undetectable, but in patients with detectable serum Tg concentrations, particularly if these are below 50 ng/ml, a scan is important to assess and localise tumour uptake of iodine before advising treatmet with iodine-131."}
{"id": "PMID:476435", "title": "Relative contributions of diet and sunlight to vitamin D state in the elderly.", "content": "In winter the vitamin D state of elderly people may reach levels associated with osteomalacia, although the disease may not be clinically apparent. A statistical correlation was observed in a group of elderly subjects during the winter between dietary vitamin D intake and vitamin D state, but the intake was generally too low to make a biologically important contribution to maintaining vitamin D concentrations. Ultraviolet light (UVL) is the primary determinant of vitamin D state in summer and winter, in winter owing to the pools of vitamin D built up during the previous summer. Plasma concentrations of 25-hydroxy vitamin D (25-OHD) in winter of 15.0-22.5 nmol/l (6-9 ng/ml) require that the concentration in the previous summer was over 40 nmol/l (16 ng/ml). To maintain plasma concentrations in the elderly above those associated with osteomalacia a mean dietary vitamin D intake of over 5 microgram/day is required. A more physiological approach, however, would be to increase exposure to UVL.", "contents": "Relative contributions of diet and sunlight to vitamin D state in the elderly. In winter the vitamin D state of elderly people may reach levels associated with osteomalacia, although the disease may not be clinically apparent. A statistical correlation was observed in a group of elderly subjects during the winter between dietary vitamin D intake and vitamin D state, but the intake was generally too low to make a biologically important contribution to maintaining vitamin D concentrations. Ultraviolet light (UVL) is the primary determinant of vitamin D state in summer and winter, in winter owing to the pools of vitamin D built up during the previous summer. Plasma concentrations of 25-hydroxy vitamin D (25-OHD) in winter of 15.0-22.5 nmol/l (6-9 ng/ml) require that the concentration in the previous summer was over 40 nmol/l (16 ng/ml). To maintain plasma concentrations in the elderly above those associated with osteomalacia a mean dietary vitamin D intake of over 5 microgram/day is required. A more physiological approach, however, would be to increase exposure to UVL."}
{"id": "PMID:476444", "title": "Patients' knowledge of their condition and treatment: how it might be improved.", "content": "A study was carried out in which patients were asked questions to assess their knowledge of their condition and treatment, the questions relating particularly to care before siting an intravenous infusion and preoperative care. A high proportion of patients knew little about the procedure they were about to undergo. It is recommended that patients should be told more about matters relating to their condition and treatment. Written information could be given to supplement that given verbally by staff. A check list might be drawn up to ensure that patients receive all relevant information.", "contents": "Patients' knowledge of their condition and treatment: how it might be improved. A study was carried out in which patients were asked questions to assess their knowledge of their condition and treatment, the questions relating particularly to care before siting an intravenous infusion and preoperative care. A high proportion of patients knew little about the procedure they were about to undergo. It is recommended that patients should be told more about matters relating to their condition and treatment. Written information could be given to supplement that given verbally by staff. A check list might be drawn up to ensure that patients receive all relevant information."}
{"id": "PMID:476446", "title": "Fetal malnutrition--the price of upright posture?", "content": "The pattern of preterm fetal growth faltering, normally seen in man, differs from that observed in animals. This type of fetal growth cannot be considered as an adaptation to facilitate birth but is more likely to be due to rapid evolution and imperfect adaptation to the upright posture. The pattern of posture and physical activity during pregnancy may therefore be an important determinant of fetal growth. Differences in intrauterine nutrition existing between social groups, usually ascribed to variations of maternal diet and nutrition, may well result from different patterns of maternal activity in the weeks preceding birth.", "contents": "Fetal malnutrition--the price of upright posture? The pattern of preterm fetal growth faltering, normally seen in man, differs from that observed in animals. This type of fetal growth cannot be considered as an adaptation to facilitate birth but is more likely to be due to rapid evolution and imperfect adaptation to the upright posture. The pattern of posture and physical activity during pregnancy may therefore be an important determinant of fetal growth. Differences in intrauterine nutrition existing between social groups, usually ascribed to variations of maternal diet and nutrition, may well result from different patterns of maternal activity in the weeks preceding birth."}
{"id": "PMID:476480", "title": "Spinal cord projections from the medial cerebellar nucleus in tree shrew (Tupaia glis).", "content": "Electrolytic lesions were placed in the medial cerebellar nucleus in tree shrews (Tupaia glis) or in fibers issuing from this nucleus. Brains and spinal cords were processed according to Fink-Heimer procedure following survival times of 2-7 days. In control animals lesions were placed in the cerebellar cortex and, in one case, in the olfactory bulb. Degenerating fibers were seen entering the cervical spinal cord and continuing to thoracic and lumbar levels. The projection is relatively profuse in the cervical cord, becoming sparse as the fibers proceed to more caudal levels. Fibers run in the lateral funiculus, predominantly contralateral to the lesion. Some fibers are observed to travel directly through the intermediate gray matter of the spinal cord. Preterminal degeneration is seen primarily in the intermediate gray of the spinal cord. Results are discussed in relation to typical locomotor behavior of tree shrew.", "contents": "Spinal cord projections from the medial cerebellar nucleus in tree shrew (Tupaia glis). Electrolytic lesions were placed in the medial cerebellar nucleus in tree shrews (Tupaia glis) or in fibers issuing from this nucleus. Brains and spinal cords were processed according to Fink-Heimer procedure following survival times of 2-7 days. In control animals lesions were placed in the cerebellar cortex and, in one case, in the olfactory bulb. Degenerating fibers were seen entering the cervical spinal cord and continuing to thoracic and lumbar levels. The projection is relatively profuse in the cervical cord, becoming sparse as the fibers proceed to more caudal levels. Fibers run in the lateral funiculus, predominantly contralateral to the lesion. Some fibers are observed to travel directly through the intermediate gray matter of the spinal cord. Preterminal degeneration is seen primarily in the intermediate gray of the spinal cord. Results are discussed in relation to typical locomotor behavior of tree shrew."}
{"id": "PMID:476481", "title": "Action of gamma-aminobutyric acid on hypothalamo-neurohypophysial axons.", "content": "gamma-Aminobutyric acid (GABA) and the related compound, muscimol, were bath-applied to a preparation containing the rat median eminence, pituitary stalk and neurohypophysis, and are shown to reversibly reduce the amplitude, and increase the latency, of the antidromic compound action potential recorded from hypothalamoneurohypophysial axons in response to electrical stimulation of the posterior pituitary lobe. Both actions were antagonized by bicuculline, but were unaffected by strychnine. The data suggest that the peptidergic axons of the hypothalamo-neurohypophysial tract are endowed with receptors for GABA, which is believed to be synthesized and released by neurohypophysial glial cells.", "contents": "Action of gamma-aminobutyric acid on hypothalamo-neurohypophysial axons. gamma-Aminobutyric acid (GABA) and the related compound, muscimol, were bath-applied to a preparation containing the rat median eminence, pituitary stalk and neurohypophysis, and are shown to reversibly reduce the amplitude, and increase the latency, of the antidromic compound action potential recorded from hypothalamoneurohypophysial axons in response to electrical stimulation of the posterior pituitary lobe. Both actions were antagonized by bicuculline, but were unaffected by strychnine. The data suggest that the peptidergic axons of the hypothalamo-neurohypophysial tract are endowed with receptors for GABA, which is believed to be synthesized and released by neurohypophysial glial cells."}
{"id": "PMID:476482", "title": "Identification of direct competition for, and indirect influences on, striatal muscarinic cholinergic receptors: in vivo [3H]quinuclidinyl benzilate binding in rats.", "content": "[3H]Quinuclidinyl benzilate (QNB) binds to specific muscarinic receptors of rat striatum, in vivo. The binding is saturable and displaceable by muscarinic drugs. Clozapine and thioridazine are unique antipsychotic agents with low liability for extrapyramidal side-effects, and both displaced ONB, while several other neuroleptics did not. In addition to this apparent direct competition for cholinergic receptors, morphine and amphetamine increased ONB binding by indirect influences on muscarinic receptors. In vivo QNB binding not only confirms in vitro findings, but it also detects indirect, probably transsynaptic, alterations of muscarinic cholinergic receptor dynamics.", "contents": "Identification of direct competition for, and indirect influences on, striatal muscarinic cholinergic receptors: in vivo [3H]quinuclidinyl benzilate binding in rats. [3H]Quinuclidinyl benzilate (QNB) binds to specific muscarinic receptors of rat striatum, in vivo. The binding is saturable and displaceable by muscarinic drugs. Clozapine and thioridazine are unique antipsychotic agents with low liability for extrapyramidal side-effects, and both displaced ONB, while several other neuroleptics did not. In addition to this apparent direct competition for cholinergic receptors, morphine and amphetamine increased ONB binding by indirect influences on muscarinic receptors. In vivo QNB binding not only confirms in vitro findings, but it also detects indirect, probably transsynaptic, alterations of muscarinic cholinergic receptor dynamics."}
{"id": "PMID:476483", "title": "Alterations in uptake and metabolism of aspartate and glutamate in brain of thiamine deficient animals.", "content": "The high affinity uptake systems of aspartate, glutamate, glycine and taurine were studied in synaptosomal preparations isolated from the cerebellum, medulla-pons and the telencephalon of rats made thiamine deficient (TD) by diet or pyrithiamine (PT). There was a significant enhancement in the uptake of asparate/glutamate (probably transported by the same carrier) by the synaptosomal preparations of the cerebellum only, in both groups of thiamine-deficient animals as compared to controls. This was due to an increase in the number of uptake sites and not to an alteration of the binding affinity. Aspartate levels decreased significantly in all three brain areas of PT-treated animals and this change was greatest in the medulla-pons and the cerebellum and least in the cortex. Glutamate and serine levels were significantly decreased only in the medulla-pons whilst the concentration of glutamine was significantly increased in the three brain regions studied. The changes in both uptake and levels of amino acids in TD rats were reversed by thiamine therapy. Though the uptake studies do not discriminate between an altered aspartergic or glutamergic system, the changes in the levels of aspartate in the cerebellum suggest that the aspartergic system is involved. Since earlier studies showed a selective impairment in the high affinity uptake of serotonin by cerebellar synaptosomes, thiamine deficiency could impair cerebellar function by inducing an imbalance in its neurotransmitter systems.", "contents": "Alterations in uptake and metabolism of aspartate and glutamate in brain of thiamine deficient animals. The high affinity uptake systems of aspartate, glutamate, glycine and taurine were studied in synaptosomal preparations isolated from the cerebellum, medulla-pons and the telencephalon of rats made thiamine deficient (TD) by diet or pyrithiamine (PT). There was a significant enhancement in the uptake of asparate/glutamate (probably transported by the same carrier) by the synaptosomal preparations of the cerebellum only, in both groups of thiamine-deficient animals as compared to controls. This was due to an increase in the number of uptake sites and not to an alteration of the binding affinity. Aspartate levels decreased significantly in all three brain areas of PT-treated animals and this change was greatest in the medulla-pons and the cerebellum and least in the cortex. Glutamate and serine levels were significantly decreased only in the medulla-pons whilst the concentration of glutamine was significantly increased in the three brain regions studied. The changes in both uptake and levels of amino acids in TD rats were reversed by thiamine therapy. Though the uptake studies do not discriminate between an altered aspartergic or glutamergic system, the changes in the levels of aspartate in the cerebellum suggest that the aspartergic system is involved. Since earlier studies showed a selective impairment in the high affinity uptake of serotonin by cerebellar synaptosomes, thiamine deficiency could impair cerebellar function by inducing an imbalance in its neurotransmitter systems."}
{"id": "PMID:476484", "title": "The effect of phenytoin on the action potential of a vertebrate spinal neuron.", "content": "The effect of phenytoin (PTN) 20 microgram/ml was tested on the passive membrane properties and the action potential of the dorsal cells in the spinal cord of the river lamprey. Dorsal cells are primary sensory neurons with no synaptic input, and thus allow examination of membrane properties in the absence of contaminating synaptic currents. PTN did not affect the resting membrane potential and slightly raised the input resistance. However, it greatly raised the threshold voltage and current for activation of action potentials by intracellularly injected current. It also reduced the maximum rate of rise of the action potential, the spike overshoot and the spike undershoot, while increasing the spike duration. In contrast to findings in other vertebrate sensory neurons, dorsal cell action potentials were blocked in zero sodium or tetrodotoxin, and not affected by zero calcium or 1 mM manganese. Thus they PTN on dorsal cells is that it partially blocks the activated sodium conductance increase of the action potential. Because a long delay was observed for maximal effect of PTN and for washout, it is postulated that the drug may require partitioning into the lipid membrane, or entry into the cell for its pharmacological action.", "contents": "The effect of phenytoin on the action potential of a vertebrate spinal neuron. The effect of phenytoin (PTN) 20 microgram/ml was tested on the passive membrane properties and the action potential of the dorsal cells in the spinal cord of the river lamprey. Dorsal cells are primary sensory neurons with no synaptic input, and thus allow examination of membrane properties in the absence of contaminating synaptic currents. PTN did not affect the resting membrane potential and slightly raised the input resistance. However, it greatly raised the threshold voltage and current for activation of action potentials by intracellularly injected current. It also reduced the maximum rate of rise of the action potential, the spike overshoot and the spike undershoot, while increasing the spike duration. In contrast to findings in other vertebrate sensory neurons, dorsal cell action potentials were blocked in zero sodium or tetrodotoxin, and not affected by zero calcium or 1 mM manganese. Thus they PTN on dorsal cells is that it partially blocks the activated sodium conductance increase of the action potential. Because a long delay was observed for maximal effect of PTN and for washout, it is postulated that the drug may require partitioning into the lipid membrane, or entry into the cell for its pharmacological action."}
{"id": "PMID:476490", "title": "Origin of descending afferents to the rostral part of dorsal cap of inferior olive which transfers contralateral optic activities to the flocculus. A horseradish peroxidase study.", "content": "Neurons in the ventromedial tegmental area that project to the rostral part of the dorsal cap of the ipsilateral inferior olive were labeled by the technique of retrograde transport of horseradish peroxidase (HRP). Following iontophoresis of HRP into the rostral part of the dorsal cap a cluster of labeled cells are found ipsilaterally in the area ventromedial to the red nucleus as well as in the area between the substantia nigra and the medial lemniscus. Following HRP injections into the medial accessory olive just beneath the dorsal cap, labeled cells are found exclusively contralaterally in the deep layers of the superior colliculus and in the adjacent reticular formation. These findings clearly show that neuronal groups in the ventromedial tegmental area are involved in the pathway which from the contralateral eye via the rostral half of the dorsal cap conveys impulses to the flocculus, recorded as climbing fiber activation.", "contents": "Origin of descending afferents to the rostral part of dorsal cap of inferior olive which transfers contralateral optic activities to the flocculus. A horseradish peroxidase study. Neurons in the ventromedial tegmental area that project to the rostral part of the dorsal cap of the ipsilateral inferior olive were labeled by the technique of retrograde transport of horseradish peroxidase (HRP). Following iontophoresis of HRP into the rostral part of the dorsal cap a cluster of labeled cells are found ipsilaterally in the area ventromedial to the red nucleus as well as in the area between the substantia nigra and the medial lemniscus. Following HRP injections into the medial accessory olive just beneath the dorsal cap, labeled cells are found exclusively contralaterally in the deep layers of the superior colliculus and in the adjacent reticular formation. These findings clearly show that neuronal groups in the ventromedial tegmental area are involved in the pathway which from the contralateral eye via the rostral half of the dorsal cap conveys impulses to the flocculus, recorded as climbing fiber activation."}
{"id": "PMID:476491", "title": "A comparison of the effect of mid-thoracic spinal hemisection in the neonatal or weanling rat on the distribution and density of dorsal root axons in the lumbosacral spinal cord of the adult.", "content": "Transecting the thoracic spinal cord of the rat has markedly different effects on behavioral responses of the hindlimbs if the lesion is made at the neonatal or weanling stage of development. The present investigation tested the possibility that the behavioral differences were related to a difference in the distribution or density of dorsal root connections in the lumbosacral spinal cord. In order to use each animal as its own control the distribution and density of dorsal root axons was compared on the two sides of the L5-S1 segments of the lumbosacral spinal cord in adult rats given a mid-thoracic spinal hemisection at the neonatal or weanling stage of development. Comparing the experimental (initially hemisected side) and control sides of the cord, we found no evidence for a change in the distribution of dorsal root axons. The distribution of Fink-Heimer stained degeneration 4--6 days after bilateral spinal root section was virtually identical on the two sides of the cord from animals hemisected at either stage. However, in rats spinally hemisected at the neonatal stage (n = 8), a significantly greater density of dorsal root degeneration was found within the intermediate nucleus of Cajal (INC) on the experimental side using coded material and a blind analysis. No difference in the density of dorsal root degeneration was detected in the group of rats spinally hemisected at the weanling stage (n = 6). Controls indicated that the increased density of degeneration was not due to compression resulting from shrinkage of the INC or to degeneration remaining from the initial hemisection. We conclude that the increased amount of argyrophilia within the INC of neonatally hemisected rats is due to an increased density of dorsal root axons in this zone. This result supports the hypothesis that the behavioral differences found when comparing animals transected at the neonatal or weanling stages of development are related to an increased number of dorsal root connections within the lumbosacral spinal cord.", "contents": "A comparison of the effect of mid-thoracic spinal hemisection in the neonatal or weanling rat on the distribution and density of dorsal root axons in the lumbosacral spinal cord of the adult. Transecting the thoracic spinal cord of the rat has markedly different effects on behavioral responses of the hindlimbs if the lesion is made at the neonatal or weanling stage of development. The present investigation tested the possibility that the behavioral differences were related to a difference in the distribution or density of dorsal root connections in the lumbosacral spinal cord. In order to use each animal as its own control the distribution and density of dorsal root axons was compared on the two sides of the L5-S1 segments of the lumbosacral spinal cord in adult rats given a mid-thoracic spinal hemisection at the neonatal or weanling stage of development. Comparing the experimental (initially hemisected side) and control sides of the cord, we found no evidence for a change in the distribution of dorsal root axons. The distribution of Fink-Heimer stained degeneration 4--6 days after bilateral spinal root section was virtually identical on the two sides of the cord from animals hemisected at either stage. However, in rats spinally hemisected at the neonatal stage (n = 8), a significantly greater density of dorsal root degeneration was found within the intermediate nucleus of Cajal (INC) on the experimental side using coded material and a blind analysis. No difference in the density of dorsal root degeneration was detected in the group of rats spinally hemisected at the weanling stage (n = 6). Controls indicated that the increased density of degeneration was not due to compression resulting from shrinkage of the INC or to degeneration remaining from the initial hemisection. We conclude that the increased amount of argyrophilia within the INC of neonatally hemisected rats is due to an increased density of dorsal root axons in this zone. This result supports the hypothesis that the behavioral differences found when comparing animals transected at the neonatal or weanling stages of development are related to an increased number of dorsal root connections within the lumbosacral spinal cord."}
{"id": "PMID:476492", "title": "Nicotinic cholinergic receptors in brain synaptosomes.", "content": "In order to elucidate pharmacological characteristics of nicotinic receptors in the brain, competitive binding of nicotine analogues or cholinergic agents to brain particles was studied utilizing [3H]nicotine. The binding of [3H]nicotine to brain crude nitochondrial or synaptosomal fraction was progressively inhibited by the addition of increasing amounts of native nicotine or nornicotine, but cotinine had little effect. Of the myelin, synaptosomal and mitochondrial subfractions of the crude mitochondrial fraction, [3H]nicotine binding was almost exclusively confined to synaptosomes. In this binding, the affinity constant was 1.97 X 10(9)/M and the maximum binding capacity was 7.30 X 40(-8) M/g of protein. This binding was reduced by 50% in the presence of 4 X 10(-7) M D-tubocurarine and 10(-6) M carbamylcholine, while atropine was the least effective of the drugs tested. These findings suggest the possible existence of nicotinic cholinergic receptors in the synaptic region. A study of regional differences in nicotinic cholinergic receptors showed that higher specific bindings of nicotine to synaptosomes occurred in the hypothalamus, hippocampus and thalamus. These findings suggest that the nicotinic cholinergic mechanism plays an important role in these regions of the central nervous system.", "contents": "Nicotinic cholinergic receptors in brain synaptosomes. In order to elucidate pharmacological characteristics of nicotinic receptors in the brain, competitive binding of nicotine analogues or cholinergic agents to brain particles was studied utilizing [3H]nicotine. The binding of [3H]nicotine to brain crude nitochondrial or synaptosomal fraction was progressively inhibited by the addition of increasing amounts of native nicotine or nornicotine, but cotinine had little effect. Of the myelin, synaptosomal and mitochondrial subfractions of the crude mitochondrial fraction, [3H]nicotine binding was almost exclusively confined to synaptosomes. In this binding, the affinity constant was 1.97 X 10(9)/M and the maximum binding capacity was 7.30 X 40(-8) M/g of protein. This binding was reduced by 50% in the presence of 4 X 10(-7) M D-tubocurarine and 10(-6) M carbamylcholine, while atropine was the least effective of the drugs tested. These findings suggest the possible existence of nicotinic cholinergic receptors in the synaptic region. A study of regional differences in nicotinic cholinergic receptors showed that higher specific bindings of nicotine to synaptosomes occurred in the hypothalamus, hippocampus and thalamus. These findings suggest that the nicotinic cholinergic mechanism plays an important role in these regions of the central nervous system."}
{"id": "PMID:476493", "title": "Dissociation of active from immobility components of sexual behavior in female rats by central 6-hydroxydopamine: implications for CA involvement in sexual behavior and sensorimotor responsiveness.", "content": "Ovariectomized female rats were given a hormone treatment (2 X 8 micrograms/kg estradiol benzoate) that normally supports only low levels of lordosis responding and no soliciting behavior in tests with sexually active males. When subjected to an intraventricular 6-hydroxydopamine (6-OHDA) procedure (with pargyline pretreatment) that produced 85% and 95% depletions of caudate dopamine and cortical norepinephrine respectively, these females exhibited a dramatic increase in the intensity and frequency of lordotic responding but no soliciting behavior over 3 weekly tests. The increase in lordosis was not due to a drug- or stress-induced release of adrenal progesterone, since dexamethasone suppressed the progesterone levels, as documented by radioimmunoassay, but not the higher receptivity of 6-OHDA treated females. In other ovariectomized females given a hormone regimen (2 X 50 micrograms/kg estradiol benzoate plus 500 micrograms progesterone) that supported maximal levels of lordosis and soliciting, the same 6-OHDA treatment prolonged the average duration of lordosis while actually decreasing the incidence and duration of soliciting. The hypothesis is put forward that the differential effects of interfering with catecholamine, and more likely dopamine function on the soliciting and lordosis components of female sexual behavior might best be understood as a dissociation between mutually antagonistic behavior patterns such that responsiveness involving active orientation and forward locomotion is suppressed, whereas responses requiring immobility are augmented.", "contents": "Dissociation of active from immobility components of sexual behavior in female rats by central 6-hydroxydopamine: implications for CA involvement in sexual behavior and sensorimotor responsiveness. Ovariectomized female rats were given a hormone treatment (2 X 8 micrograms/kg estradiol benzoate) that normally supports only low levels of lordosis responding and no soliciting behavior in tests with sexually active males. When subjected to an intraventricular 6-hydroxydopamine (6-OHDA) procedure (with pargyline pretreatment) that produced 85% and 95% depletions of caudate dopamine and cortical norepinephrine respectively, these females exhibited a dramatic increase in the intensity and frequency of lordotic responding but no soliciting behavior over 3 weekly tests. The increase in lordosis was not due to a drug- or stress-induced release of adrenal progesterone, since dexamethasone suppressed the progesterone levels, as documented by radioimmunoassay, but not the higher receptivity of 6-OHDA treated females. In other ovariectomized females given a hormone regimen (2 X 50 micrograms/kg estradiol benzoate plus 500 micrograms progesterone) that supported maximal levels of lordosis and soliciting, the same 6-OHDA treatment prolonged the average duration of lordosis while actually decreasing the incidence and duration of soliciting. The hypothesis is put forward that the differential effects of interfering with catecholamine, and more likely dopamine function on the soliciting and lordosis components of female sexual behavior might best be understood as a dissociation between mutually antagonistic behavior patterns such that responsiveness involving active orientation and forward locomotion is suppressed, whereas responses requiring immobility are augmented."}
{"id": "PMID:476494", "title": "Incorporation of [3H]leucine into hypothalamic nerve and glial cells. A comparison by EM autoradiography.", "content": "Incorporation of L-[3H]leucine into the arcuate and supraoptic nuclei of rat and mice hypothalamus was studied by EM autoradiography at 30 min after intracerebral and intravenous application, respectively. The analysis applied provides information about (1) the distribution and (2) the concentration of radioactivity in the various tissue compartments, in particular nerve and glial cells. In the supraoptic nucleus, the proportion of radioactivity present in neuronal perikarya markedly exceeds that in the neuropil. Similar amounts of radioactivity in neuronal perikarya and neuropil were found for the arcuate nucleus. In both hypothalamic nuclei, only a small percentage of radioactivity is localized in glial cell bodies. However, the concentration of radioactivity in glial cells is nearly as high as in nerve cells and is lowest in the neuropil. Furthermore analysis of the subcompartments of the neuropil reveals that a considerable proportion of its radioactivity can be attributed to astrocytic processes. Conditions are discussed under which grain densities can be taken as measurements of relative synthesis rates of proteins. The results which are at variance with earlier autoradiographic studies are largely consistent with those of more recent biochemical studies on brain tissue separated into neuronal and glial fractions.", "contents": "Incorporation of [3H]leucine into hypothalamic nerve and glial cells. A comparison by EM autoradiography. Incorporation of L-[3H]leucine into the arcuate and supraoptic nuclei of rat and mice hypothalamus was studied by EM autoradiography at 30 min after intracerebral and intravenous application, respectively. The analysis applied provides information about (1) the distribution and (2) the concentration of radioactivity in the various tissue compartments, in particular nerve and glial cells. In the supraoptic nucleus, the proportion of radioactivity present in neuronal perikarya markedly exceeds that in the neuropil. Similar amounts of radioactivity in neuronal perikarya and neuropil were found for the arcuate nucleus. In both hypothalamic nuclei, only a small percentage of radioactivity is localized in glial cell bodies. However, the concentration of radioactivity in glial cells is nearly as high as in nerve cells and is lowest in the neuropil. Furthermore analysis of the subcompartments of the neuropil reveals that a considerable proportion of its radioactivity can be attributed to astrocytic processes. Conditions are discussed under which grain densities can be taken as measurements of relative synthesis rates of proteins. The results which are at variance with earlier autoradiographic studies are largely consistent with those of more recent biochemical studies on brain tissue separated into neuronal and glial fractions."}
{"id": "PMID:476501", "title": "Mode of uptake and degradation of 125I-labelled insulin by isolated hepatocytes and H4 hepatoma cells.", "content": "The effects of various agents on the binding and degradation of 125I-labelled insulin by isolated rat hepatocytes and cultured H4 hepatoma cells were studied. Various lysosomotropic agents, including chloroquine, ammonium chloride, and the topical anesthetics, lidocaine and procaine inhibited insulin degradation by H4 hepatoma cells but had little effect on the binding of the hormone. Similarly, tosyl-L-lysyl chloromethyl ketone selectively inhibited the degradation of 125I-labelled insulin by isolated hepatocytes, as did the sulfhydryl reagents, p-hydroxy- and p-chloromercuriphenyl sulfonic acid. Inhibitors of energy production, including sodium fluoride, sodium azide, and dinitrophenol, also selectively inhibited the degradation of insulin by hepatocytes, although cyanide had no effect under the conditions used. Lectins and antimicrotubular agents, which are known to affect the mobility of plasma membrane proteins or of intracytoplasmic vesicles, selectively inhibited insulin degradation by hepatocytes to varying degrees, whereas agents which inhibit the function of microfilaments had no effect. At temperatures below 20 degrees C, insulin degradation was negligible but rose rapidly between 20 and 37 degrees C, suggesting that a membrane-related step is rate limiting in the overall degradative process. These results are all consistent with a model of insulin uptake by target tissue involving pinocytosis of receptor-bound hormone followed by intralysosomal degradation.", "contents": "Mode of uptake and degradation of 125I-labelled insulin by isolated hepatocytes and H4 hepatoma cells. The effects of various agents on the binding and degradation of 125I-labelled insulin by isolated rat hepatocytes and cultured H4 hepatoma cells were studied. Various lysosomotropic agents, including chloroquine, ammonium chloride, and the topical anesthetics, lidocaine and procaine inhibited insulin degradation by H4 hepatoma cells but had little effect on the binding of the hormone. Similarly, tosyl-L-lysyl chloromethyl ketone selectively inhibited the degradation of 125I-labelled insulin by isolated hepatocytes, as did the sulfhydryl reagents, p-hydroxy- and p-chloromercuriphenyl sulfonic acid. Inhibitors of energy production, including sodium fluoride, sodium azide, and dinitrophenol, also selectively inhibited the degradation of insulin by hepatocytes, although cyanide had no effect under the conditions used. Lectins and antimicrotubular agents, which are known to affect the mobility of plasma membrane proteins or of intracytoplasmic vesicles, selectively inhibited insulin degradation by hepatocytes to varying degrees, whereas agents which inhibit the function of microfilaments had no effect. At temperatures below 20 degrees C, insulin degradation was negligible but rose rapidly between 20 and 37 degrees C, suggesting that a membrane-related step is rate limiting in the overall degradative process. These results are all consistent with a model of insulin uptake by target tissue involving pinocytosis of receptor-bound hormone followed by intralysosomal degradation."}
{"id": "PMID:476502", "title": "Structural relationships in the two-zinc insulin hexamer.", "content": "The refinement of the crystal structure of two-Zn pig insulin using 1.5-A (1A = 0.1 nm) resolution data by Fourier and fast Fourier least-squares methods allows us to make detailed comparisons between the two independent molecules present in the two-Zn insulin dimer and to describe their interactions in the monomer, dimer, and hexamer. The main chain structures for the two molecules agree well except at the N terminus of the A chain and the C terminus of the B chain. The residues along the line of the local two-fold axes, apart from the B25 side chain, conform extremely closely to the two-fold symmetry, although the discrepancies are much more apparent away from this axis. The ability of the insulin molecule to adopt different conformations may be an important factor in the expression of its biological activity.", "contents": "Structural relationships in the two-zinc insulin hexamer. The refinement of the crystal structure of two-Zn pig insulin using 1.5-A (1A = 0.1 nm) resolution data by Fourier and fast Fourier least-squares methods allows us to make detailed comparisons between the two independent molecules present in the two-Zn insulin dimer and to describe their interactions in the monomer, dimer, and hexamer. The main chain structures for the two molecules agree well except at the N terminus of the A chain and the C terminus of the B chain. The residues along the line of the local two-fold axes, apart from the B25 side chain, conform extremely closely to the two-fold symmetry, although the discrepancies are much more apparent away from this axis. The ability of the insulin molecule to adopt different conformations may be an important factor in the expression of its biological activity."}
{"id": "PMID:476503", "title": "Effect of thyrotropin on the phosphorylation of thyroid chromosomal proteins.", "content": "Thyrotropin (TSH) stimulated the phosphorylation of histone H1 in calf thyroid slices but had no effect on other classes of histones. Phosphorylation of total phenol-soluble nonhistone chromosomal proteins was not affected by incubation with TSH. However, when these phenol-soluble nonhistone chromosomal proteins were analysed by two-dimensional gels involving isoelectrofocusing and dodecyl sulfate-polyacrylamide gel electrophoresis, TSH was shown to stimulate the phosphorylation of two specific groups of phosphoproteins with molecular weights between 35,000 and 45,000 and isoelectric points at pH values of 5.4-6.0. This increase in phosphorylation with TSH stimulation was confirmed by quantitative analysis of one-dimensional isoelectrofocusing gels.", "contents": "Effect of thyrotropin on the phosphorylation of thyroid chromosomal proteins. Thyrotropin (TSH) stimulated the phosphorylation of histone H1 in calf thyroid slices but had no effect on other classes of histones. Phosphorylation of total phenol-soluble nonhistone chromosomal proteins was not affected by incubation with TSH. However, when these phenol-soluble nonhistone chromosomal proteins were analysed by two-dimensional gels involving isoelectrofocusing and dodecyl sulfate-polyacrylamide gel electrophoresis, TSH was shown to stimulate the phosphorylation of two specific groups of phosphoproteins with molecular weights between 35,000 and 45,000 and isoelectric points at pH values of 5.4-6.0. This increase in phosphorylation with TSH stimulation was confirmed by quantitative analysis of one-dimensional isoelectrofocusing gels."}
{"id": "PMID:476504", "title": "Immunological and biological activities of fragments and analogs of bradykinin.", "content": "Using a number of analogs and fragments of a short-chain peptide bradykinin, a series of experiments have been carried out to assess the effect of modifications to the basic structure of the parent molecule on its myotropic and immunoreactive properties. Binding kinetics of both an antibody raised against the authentic nonapeptide and its specific biological receptor found in the guinea pig ileum were used to study these alteration effects. Peptide derivatives of bradykinin with an extension at the N-terminal (Lys- and Met-Lys-bradykinin) cross-react with the antibody raised to bradykinin 59 and 70% respectively. On the other hand, internal fragments with intact C-termini (2-9 and 3-9 bradykinin) react with this same antibody to an extent of 250 and 875% respectively, indicating that they are more potent antigens than the vasopressor molecule itself. Other internal fragments, as well as 9-substituted analogs effectively and not interact. These results indicated that the C terminal arginine of bradykinin is indeed essential in the binding mechanism with its antibody. This in turn illustrates the role of the carrier ovalbumin in the development of antiserum to the ovalbumin-toluene-diisocyanate-bradykinin complex. The physiological experiments with the guinea pig bioassay preparations lead to similar conclusions. Most internal fragments of bradykinin are devoid of activity, whereas N-terminal fragments (2-9, 3-9, and 5-9 bradykinin) have retained some activity again indicating a need for an intact arginine residue at the C-terminus of the molecule. Any modification in position 9 results in severe impairment of biological activity. Thus, the C-terminal residue of bradykinin must be conserved in order that the molecule may retain its immunological and physiological activities. Any extensions, deletions, or modifications of this site will severely retard these functions.", "contents": "Immunological and biological activities of fragments and analogs of bradykinin. Using a number of analogs and fragments of a short-chain peptide bradykinin, a series of experiments have been carried out to assess the effect of modifications to the basic structure of the parent molecule on its myotropic and immunoreactive properties. Binding kinetics of both an antibody raised against the authentic nonapeptide and its specific biological receptor found in the guinea pig ileum were used to study these alteration effects. Peptide derivatives of bradykinin with an extension at the N-terminal (Lys- and Met-Lys-bradykinin) cross-react with the antibody raised to bradykinin 59 and 70% respectively. On the other hand, internal fragments with intact C-termini (2-9 and 3-9 bradykinin) react with this same antibody to an extent of 250 and 875% respectively, indicating that they are more potent antigens than the vasopressor molecule itself. Other internal fragments, as well as 9-substituted analogs effectively and not interact. These results indicated that the C terminal arginine of bradykinin is indeed essential in the binding mechanism with its antibody. This in turn illustrates the role of the carrier ovalbumin in the development of antiserum to the ovalbumin-toluene-diisocyanate-bradykinin complex. The physiological experiments with the guinea pig bioassay preparations lead to similar conclusions. Most internal fragments of bradykinin are devoid of activity, whereas N-terminal fragments (2-9, 3-9, and 5-9 bradykinin) have retained some activity again indicating a need for an intact arginine residue at the C-terminus of the molecule. Any modification in position 9 results in severe impairment of biological activity. Thus, the C-terminal residue of bradykinin must be conserved in order that the molecule may retain its immunological and physiological activities. Any extensions, deletions, or modifications of this site will severely retard these functions."}
{"id": "PMID:476505", "title": "Effect of nucleotides on UDP-N-acetylglucosamine pyrophosphatase and N-acetylglucosaminyltransferase activities in microsomal membranes.", "content": "Rat liver microsomes solubilized by incubating with lysolecithin or Triton X-100 showed very active UDP-N-acetylglucosamine pyrophosphatase activity leading to the hydrolysis of the substrate into N-acetylglucosamine-P and N-acetylglucosamine. ATP, GTP, CDPcholine, and CDPglucose exerted a considerable inhibitory effect on the solubilized membrane pyrophosphatase activity. CDPcholine and CDPglucose, in addition, appeared to stimulate the transfer of N-acetylglucosamine into endogenous and exogenous acceptor proteins. Evidence is also presented of an inhibitory effect of ATP (and to some extent GTP) on N-acetylglucosaminyltransferase activity. This inhibitory effect of ATP and GTP became clearly evident when the pyrophosphatase activity in the membranes was virtually eliminated in the presence of CDP-choline and CDPglucose. The effect of ATP and GTP on the solubilized membrane enzymes indicated that the inhibition of pyrophosphatase activity alone did not determine the rate of transfer of sugar to protein. The results also suggested that the UDP-N-acetylglucosamine pyrophosphatase and N-acetylglucosaminyltransferase activities were controlled independently and the effect of each nucleotide on these enzymes should, therefore, be carefully evaluated to understood its role in glycopolymer biosynthesis. Also, a possible role of choline and its derivatives in glycoprotein synthesis is discussed.", "contents": "Effect of nucleotides on UDP-N-acetylglucosamine pyrophosphatase and N-acetylglucosaminyltransferase activities in microsomal membranes. Rat liver microsomes solubilized by incubating with lysolecithin or Triton X-100 showed very active UDP-N-acetylglucosamine pyrophosphatase activity leading to the hydrolysis of the substrate into N-acetylglucosamine-P and N-acetylglucosamine. ATP, GTP, CDPcholine, and CDPglucose exerted a considerable inhibitory effect on the solubilized membrane pyrophosphatase activity. CDPcholine and CDPglucose, in addition, appeared to stimulate the transfer of N-acetylglucosamine into endogenous and exogenous acceptor proteins. Evidence is also presented of an inhibitory effect of ATP (and to some extent GTP) on N-acetylglucosaminyltransferase activity. This inhibitory effect of ATP and GTP became clearly evident when the pyrophosphatase activity in the membranes was virtually eliminated in the presence of CDP-choline and CDPglucose. The effect of ATP and GTP on the solubilized membrane enzymes indicated that the inhibition of pyrophosphatase activity alone did not determine the rate of transfer of sugar to protein. The results also suggested that the UDP-N-acetylglucosamine pyrophosphatase and N-acetylglucosaminyltransferase activities were controlled independently and the effect of each nucleotide on these enzymes should, therefore, be carefully evaluated to understood its role in glycopolymer biosynthesis. Also, a possible role of choline and its derivatives in glycoprotein synthesis is discussed."}
{"id": "PMID:476508", "title": "Chemical synthesis of sn-3-phosphatidyl sulfocholine, a sulfonium analog of lecithin.", "content": "A sulfonium analog of lecithin has been reported to replace the ubiquitous phosphatidyl choline in a non-photosynthetic diatom, Nitzschia alba. The structure of this sulfonium analog has now been established by chemical synthesis using the following methods: (i) condensation of sn-3-phosphatidic acid (dimyristoyl-, dipalmitoyl-, distearoyl-, distearoyl-, and dioleoyl-) with sulfocholine chloride in the presence of triisopropylbenzenesulfonylchloride in chloroform-pyridine (9:1); and (ii) phosphorylation of 1,2-dipalmitoyl-sn-glycerol with monophenylphosphoryl-dichloridate followed by a reaction with sulfocholine in the presence of pyridine and finally removal of the blocking phenyl group by catalytic hydrogenolysis. The desired synthetic products were obtained in overall yields of 50-70% and 11% for methods (i) and (ii), respectively, and were characterized by elemental analyses; infrared spectroscopy, nuclear magnetic resonance spectrometry, and mass spectrometry; optical rotation; and thin-layer chromatography mobilities. Comparison of the synthetic analogs with the natural sulfolecithin showed them to be identical, except for the nature of the fatty acid chains, thus establishing the natural product as sn-3-phosphatidyl sulfocholine.", "contents": "Chemical synthesis of sn-3-phosphatidyl sulfocholine, a sulfonium analog of lecithin. A sulfonium analog of lecithin has been reported to replace the ubiquitous phosphatidyl choline in a non-photosynthetic diatom, Nitzschia alba. The structure of this sulfonium analog has now been established by chemical synthesis using the following methods: (i) condensation of sn-3-phosphatidic acid (dimyristoyl-, dipalmitoyl-, distearoyl-, distearoyl-, and dioleoyl-) with sulfocholine chloride in the presence of triisopropylbenzenesulfonylchloride in chloroform-pyridine (9:1); and (ii) phosphorylation of 1,2-dipalmitoyl-sn-glycerol with monophenylphosphoryl-dichloridate followed by a reaction with sulfocholine in the presence of pyridine and finally removal of the blocking phenyl group by catalytic hydrogenolysis. The desired synthetic products were obtained in overall yields of 50-70% and 11% for methods (i) and (ii), respectively, and were characterized by elemental analyses; infrared spectroscopy, nuclear magnetic resonance spectrometry, and mass spectrometry; optical rotation; and thin-layer chromatography mobilities. Comparison of the synthetic analogs with the natural sulfolecithin showed them to be identical, except for the nature of the fatty acid chains, thus establishing the natural product as sn-3-phosphatidyl sulfocholine."}
{"id": "PMID:476507", "title": "On hepatic mitochondrial monoamine oxidase activity in lipid deficiency.", "content": "A marked decrease in liver mitochondrial monoamine oxidase activity was noticed in rats fed a fat-free diet as compared with that of their controls. In lipid-deprived rats, the specific activity of this enzyme was very low towards different substrates studied. The activity of kynurenine 3-monooxygenase, which like monoamine oxidase is localized on the mitochondrial outer membrane, was similarly depressed under conditions of lipid deprivation. On the other hand no major changes were observed in the activity of the inner membrane enzyme, kynurenine amino-transferase. Mitochondria from fat-free diet-fed rats were deficient in essential fatty acids whereas no appreciable variations were found in the relative proportions of phospholipids in comparison with those of control mitochondria. Mitochondrial monoamine oxidase activity of the deficient rats retained its sensitivities to inhibitor drugs like clorgyline and deprenyl. No changes were noticeable in the substrate specificity of monoamine oxidase in these rats. When we switched the fat-free diet-fed rats to a diet supplemented with a source of essential fatty acids, there was an elevation in the activities of both monoamine oxidase and kynurenine 3-monooxygenase, their levels approaching those of the control rats.", "contents": "On hepatic mitochondrial monoamine oxidase activity in lipid deficiency. A marked decrease in liver mitochondrial monoamine oxidase activity was noticed in rats fed a fat-free diet as compared with that of their controls. In lipid-deprived rats, the specific activity of this enzyme was very low towards different substrates studied. The activity of kynurenine 3-monooxygenase, which like monoamine oxidase is localized on the mitochondrial outer membrane, was similarly depressed under conditions of lipid deprivation. On the other hand no major changes were observed in the activity of the inner membrane enzyme, kynurenine amino-transferase. Mitochondria from fat-free diet-fed rats were deficient in essential fatty acids whereas no appreciable variations were found in the relative proportions of phospholipids in comparison with those of control mitochondria. Mitochondrial monoamine oxidase activity of the deficient rats retained its sensitivities to inhibitor drugs like clorgyline and deprenyl. No changes were noticeable in the substrate specificity of monoamine oxidase in these rats. When we switched the fat-free diet-fed rats to a diet supplemented with a source of essential fatty acids, there was an elevation in the activities of both monoamine oxidase and kynurenine 3-monooxygenase, their levels approaching those of the control rats."}
{"id": "PMID:476509", "title": "Lipid requirements for the aggregation of CTP:phosphocholine cytidylyltransferase in rat liver cytosol.", "content": "Two forms of CTP:phosphocholine cytidylyltransferase were identified in rat liver cytosol by gel filtration chromatography. The low molecular weight form (L form) is the major form in fresh cytosol. The enzyme associates into a high molecular weight form (H form) upon storage of the cytosol at 4 degrees C. Aggregation of the purified L form of cytidylyltransferase is caused by total rat liver lipids, neutral lipids, diacylglycerol, or phosphatidylglycerol. Diacylglycerol was the only lipid isolated from the rat liver that caused aggregation of the purified enzyme. Although the addition of diacylglycerol to the cytosol did not change the amount of aggregation of the enzyme, a 2.5-fold increase in H form was observed in cytosol pretreated with phospholipase C, or in cytosol from rats fed a high cholesterol diet. In both of these cytosolic preparations, the concentration of diacylglycerol was elevated twofold. Phosphatidylglycerol did not seem to affect the association of the enzyme in cytosol since it is present in very low concentrations in the rat liver cytosol, and its degradation in cytosol by a specific phospholipase did not affect the rate of aggregation. The results suggest that diacylglycerol in an appropriate form is required for association of cytidylyltransferase in rat liver cytosol.", "contents": "Lipid requirements for the aggregation of CTP:phosphocholine cytidylyltransferase in rat liver cytosol. Two forms of CTP:phosphocholine cytidylyltransferase were identified in rat liver cytosol by gel filtration chromatography. The low molecular weight form (L form) is the major form in fresh cytosol. The enzyme associates into a high molecular weight form (H form) upon storage of the cytosol at 4 degrees C. Aggregation of the purified L form of cytidylyltransferase is caused by total rat liver lipids, neutral lipids, diacylglycerol, or phosphatidylglycerol. Diacylglycerol was the only lipid isolated from the rat liver that caused aggregation of the purified enzyme. Although the addition of diacylglycerol to the cytosol did not change the amount of aggregation of the enzyme, a 2.5-fold increase in H form was observed in cytosol pretreated with phospholipase C, or in cytosol from rats fed a high cholesterol diet. In both of these cytosolic preparations, the concentration of diacylglycerol was elevated twofold. Phosphatidylglycerol did not seem to affect the association of the enzyme in cytosol since it is present in very low concentrations in the rat liver cytosol, and its degradation in cytosol by a specific phospholipase did not affect the rate of aggregation. The results suggest that diacylglycerol in an appropriate form is required for association of cytidylyltransferase in rat liver cytosol."}
{"id": "PMID:476510", "title": "Suitability of L-[35S]methionine for studying the biosynthesis of the polypeptides of mouse liver endoplasmic reticulum membrane fractions in vivo.", "content": "The use of L-[35S]methionine (500-700 Ci/mmol (1 Ci = 37 GBq) for labelling the polypeptides of liver rough (R) and smooth (S)endoplasmic reticulum (ER) membrane fractions in vivo was studied. Adult mice were injected intraperitoneally with 400 muCi of the isotope and killed at various times (2'min to 24 h) thereafter. RER and SER fractions were prepared, stripped of ribosomes, and treated with Triton X-100 to remove intravesicular contents. Sufficient radioactivity was present in individual aliquots (75 microgram protein) of the ER membrane fractions to permit their analysis by fluorography after separation by electrophoresis in polyacrylamide gels containing sodium dodecyl sulphate. By 3 min, although the majority of the labelled components were of intravesicular origin, some 12 membrane polypeptides were labelled in the RER fraction (including one corresponding in migration to cytochrome P-450); some 6 of these latter polypeptides were labelled to a lesser degree in the SER membrane fraction at this time. By 5 min, the patterns of radioactive polypeptides of the RER and SER fractions (including both membrane and intravesicular components) were identical. By 7 min, some 28 labelled membrane polypeptides were detectable in the total microsomal membrane. Analysis of the 24-h samples revealed that all the membrane polypeptides seen by staining with Coomassie blue were visualised by fluorography. Other studies revealed the applicability of the approach used for producing highly labelled cell sap and serum proteins. The overall results demonstrate the suitability of L-[35S]methionine administered in vivo for producing mouse liver ER membrane polypeptides of relatively high radioactivity and are consistent with a rapid conversion of RER to SER by ribosome detachment or membrane flow.", "contents": "Suitability of L-[35S]methionine for studying the biosynthesis of the polypeptides of mouse liver endoplasmic reticulum membrane fractions in vivo. The use of L-[35S]methionine (500-700 Ci/mmol (1 Ci = 37 GBq) for labelling the polypeptides of liver rough (R) and smooth (S)endoplasmic reticulum (ER) membrane fractions in vivo was studied. Adult mice were injected intraperitoneally with 400 muCi of the isotope and killed at various times (2'min to 24 h) thereafter. RER and SER fractions were prepared, stripped of ribosomes, and treated with Triton X-100 to remove intravesicular contents. Sufficient radioactivity was present in individual aliquots (75 microgram protein) of the ER membrane fractions to permit their analysis by fluorography after separation by electrophoresis in polyacrylamide gels containing sodium dodecyl sulphate. By 3 min, although the majority of the labelled components were of intravesicular origin, some 12 membrane polypeptides were labelled in the RER fraction (including one corresponding in migration to cytochrome P-450); some 6 of these latter polypeptides were labelled to a lesser degree in the SER membrane fraction at this time. By 5 min, the patterns of radioactive polypeptides of the RER and SER fractions (including both membrane and intravesicular components) were identical. By 7 min, some 28 labelled membrane polypeptides were detectable in the total microsomal membrane. Analysis of the 24-h samples revealed that all the membrane polypeptides seen by staining with Coomassie blue were visualised by fluorography. Other studies revealed the applicability of the approach used for producing highly labelled cell sap and serum proteins. The overall results demonstrate the suitability of L-[35S]methionine administered in vivo for producing mouse liver ER membrane polypeptides of relatively high radioactivity and are consistent with a rapid conversion of RER to SER by ribosome detachment or membrane flow."}
{"id": "PMID:476511", "title": "Resolution of saturated and unsaturated 5 beta-cholanoic acids by gas-liquid and thin-layer chromatography.", "content": "The thin-layer and gas-liquid chromatographic properties of the methyl ester acetates were determined for a series of 20 monounsaturated 5 beta-cholanoic acids representing the simple chemical and enzymic dehydration products of the common bile acids. The unsaturated acids were generally indistinguishable from their saturated analogues by thin-layer chromatography on plain silica gel, but resolution was achieved on silica gel impregnated with silver nitrate for compounds having sterically exposed double bonds. The gas-liquid chromatographic behaviour of the unsaturated bile acids on the OV-225, SE-30, and Poly-S-179 liquid phases was closely similar to that observed for the saturated bile acids. The 5 beta-cholenoic acids obeyed the general rules of chromatographic mobility based on the overall shape of the molecule and the number and configuration of functional groups, with a constant retention factor attributable to the olefinic bond. The structural information provided by the chromatographic behaviour of the standard unsaturated bile acids allows a distinction to be made among most of the isomeric 5 beta-cholenoates. A complete identification of all isomeric olefins is possible when chromatographic and mass spectrometric data are combined.", "contents": "Resolution of saturated and unsaturated 5 beta-cholanoic acids by gas-liquid and thin-layer chromatography. The thin-layer and gas-liquid chromatographic properties of the methyl ester acetates were determined for a series of 20 monounsaturated 5 beta-cholanoic acids representing the simple chemical and enzymic dehydration products of the common bile acids. The unsaturated acids were generally indistinguishable from their saturated analogues by thin-layer chromatography on plain silica gel, but resolution was achieved on silica gel impregnated with silver nitrate for compounds having sterically exposed double bonds. The gas-liquid chromatographic behaviour of the unsaturated bile acids on the OV-225, SE-30, and Poly-S-179 liquid phases was closely similar to that observed for the saturated bile acids. The 5 beta-cholenoic acids obeyed the general rules of chromatographic mobility based on the overall shape of the molecule and the number and configuration of functional groups, with a constant retention factor attributable to the olefinic bond. The structural information provided by the chromatographic behaviour of the standard unsaturated bile acids allows a distinction to be made among most of the isomeric 5 beta-cholenoates. A complete identification of all isomeric olefins is possible when chromatographic and mass spectrometric data are combined."}
{"id": "PMID:476512", "title": "Effects of dietary carbohydrate on cholesterol metabolism in rats and rabbits.", "content": "Semipurified diets produce a marked hypercholesterolemia in rabbits and tend to elevate the level of plasma cholesterol in rats. They also decrease rates of oxidation of [26-14C]cholesterol to respiratory 14CO2 and excretion of label in fecal lipid, compared with commercial feed. In both species, the hypercholesterolemia was prevented and the rate of oxidation of [26-14C]cholesterol increased by using unmodified potato starch as the carbohydrate component of the semipurified diets. Potato starch was poorly digested by rats but appeared to be well utilized by rabbits. A semipurifed diet containing cooked potato starch gave results in rats comparable to those obtained with diets containing other types of carbohydrate. Cooked potato starch produced diarrhea in rabbits, thus complicating interpretation of the results. When the diarrhea was treated with antibiotics, the results were similar to those obtained with other carbohydrates. Rats fed commercial diet which had been heated in an oven or autoclaved had higher plasma levels than those fed untreated commercial diet but no significant differences in rates of oxidation or excretion of cholesterol were observed.", "contents": "Effects of dietary carbohydrate on cholesterol metabolism in rats and rabbits. Semipurified diets produce a marked hypercholesterolemia in rabbits and tend to elevate the level of plasma cholesterol in rats. They also decrease rates of oxidation of [26-14C]cholesterol to respiratory 14CO2 and excretion of label in fecal lipid, compared with commercial feed. In both species, the hypercholesterolemia was prevented and the rate of oxidation of [26-14C]cholesterol increased by using unmodified potato starch as the carbohydrate component of the semipurified diets. Potato starch was poorly digested by rats but appeared to be well utilized by rabbits. A semipurifed diet containing cooked potato starch gave results in rats comparable to those obtained with diets containing other types of carbohydrate. Cooked potato starch produced diarrhea in rabbits, thus complicating interpretation of the results. When the diarrhea was treated with antibiotics, the results were similar to those obtained with other carbohydrates. Rats fed commercial diet which had been heated in an oven or autoclaved had higher plasma levels than those fed untreated commercial diet but no significant differences in rates of oxidation or excretion of cholesterol were observed."}
{"id": "PMID:476513", "title": "Binding of mitochondrial malate dehydrogenase to mitoplasts.", "content": "The binding of 14C-labelled bovine and porcine malate dehydrogenase (EC 1.1.1.37) to rat liver mitochondria and mitoplasts was examined. The bovine enzyme was found to associate nonspecifically with isolated mitochondria and sonicated mitoplasts. Scatchard plot analysis suggested a specific binding to mitoplasts of the order of 5 pmol malate dehydrogenase per milligram of mitoplast protein. Porcine malate dehydrogenase dimer but not monomer exhibited a similar binding. The results are discussed in relation to the mechanism of uptake of the enzyme by mitochondria after synthesis on cytosolic ribosomes.", "contents": "Binding of mitochondrial malate dehydrogenase to mitoplasts. The binding of 14C-labelled bovine and porcine malate dehydrogenase (EC 1.1.1.37) to rat liver mitochondria and mitoplasts was examined. The bovine enzyme was found to associate nonspecifically with isolated mitochondria and sonicated mitoplasts. Scatchard plot analysis suggested a specific binding to mitoplasts of the order of 5 pmol malate dehydrogenase per milligram of mitoplast protein. Porcine malate dehydrogenase dimer but not monomer exhibited a similar binding. The results are discussed in relation to the mechanism of uptake of the enzyme by mitochondria after synthesis on cytosolic ribosomes."}
{"id": "PMID:476514", "title": "Identification of nonhistone chromatin proteins in chromatin subunits (or mononucleosomes) devoid of histone H1.", "content": "Rat liver chromatin was digested by micrococcal nuclease. Chromatin subunits (or mononucleosomes) were isolated by sucrose density gradient and subsequently fractionated by 6% polyacrylamide gel electrophoresis into two major components. One component (MN1) of the mononucleosomes had a higher mobility, contained histones H2A, H2B, H3, H4, and shorter DNA fragments (140 base pairs) while the other (MN2) contained all five histones and longer DNA fragments (180 base pairs). Both submononucleosomes (MN1 and MN2) were found to contain nonhistone chromatin proteins (NHCP). By electrophoresis in 15% sodium dodecyl sulfate-polyacrylamide gel, 9 and 11 major fractions of NHCP were identified in the submononucleosomes MN1 and MN2, respectively. It was also observed that treatment of mononucleosomes with 0.6 M NaCl removes most of these NHCP and histone H1 except for two major NHCP which remain in the core particles.", "contents": "Identification of nonhistone chromatin proteins in chromatin subunits (or mononucleosomes) devoid of histone H1. Rat liver chromatin was digested by micrococcal nuclease. Chromatin subunits (or mononucleosomes) were isolated by sucrose density gradient and subsequently fractionated by 6% polyacrylamide gel electrophoresis into two major components. One component (MN1) of the mononucleosomes had a higher mobility, contained histones H2A, H2B, H3, H4, and shorter DNA fragments (140 base pairs) while the other (MN2) contained all five histones and longer DNA fragments (180 base pairs). Both submononucleosomes (MN1 and MN2) were found to contain nonhistone chromatin proteins (NHCP). By electrophoresis in 15% sodium dodecyl sulfate-polyacrylamide gel, 9 and 11 major fractions of NHCP were identified in the submononucleosomes MN1 and MN2, respectively. It was also observed that treatment of mononucleosomes with 0.6 M NaCl removes most of these NHCP and histone H1 except for two major NHCP which remain in the core particles."}
{"id": "PMID:476515", "title": "Chick pineal serotonin acetyltransferase: a diurnal cycle maintained in vitro and its regulation by light.", "content": "We have reproduced in vitro the diurnal cycles in levels of serotonin acetyltransferase activity found in the chick pineal gland in vivo. The more closely the lighting conditions of culture matched those under which the birds were raised, the closer was the similarity between cycles in levels of enzyme activity in vitro and in vivo. Repetitive cycles in levels of acetyltransferase activity persisted in culture for at least 4 days under a diurnal cycle of illumination, and at least 2 days in continuous darkness. When glands were explanted into culture in the light phase of a cycle, short periods of further exposure to light markedly stimulated subsequent increase of acetyltransferase in the dark (after a short lag). Prolonged exposure to light in culture markedly inhibited increase of enzyme activity. Cycles in the levels of enzyme activity in glands cultured under altered light cycles were regulated primarily by changes in illumination. However, the endogenous biological 'clock' remained at least partly entrained to the original light cycle. Increase of acetyltransferase activity in vitro was markedly stimulated by theophylline plus compound Ro. 20.1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone) under all lighting conditions. Kinetics (to the time of attaining maximum levels in situ) of the increase under diurnal lighting and in constant darkness were indistinguishable from those in vivo. A high concentration of dl-propranolol markedly stimulated an increase in acetyltransferase activity in glands cultured in constant darkness but had little effect on glands under diurnal lighting or continuous illumination.", "contents": "Chick pineal serotonin acetyltransferase: a diurnal cycle maintained in vitro and its regulation by light. We have reproduced in vitro the diurnal cycles in levels of serotonin acetyltransferase activity found in the chick pineal gland in vivo. The more closely the lighting conditions of culture matched those under which the birds were raised, the closer was the similarity between cycles in levels of enzyme activity in vitro and in vivo. Repetitive cycles in levels of acetyltransferase activity persisted in culture for at least 4 days under a diurnal cycle of illumination, and at least 2 days in continuous darkness. When glands were explanted into culture in the light phase of a cycle, short periods of further exposure to light markedly stimulated subsequent increase of acetyltransferase in the dark (after a short lag). Prolonged exposure to light in culture markedly inhibited increase of enzyme activity. Cycles in the levels of enzyme activity in glands cultured under altered light cycles were regulated primarily by changes in illumination. However, the endogenous biological 'clock' remained at least partly entrained to the original light cycle. Increase of acetyltransferase activity in vitro was markedly stimulated by theophylline plus compound Ro. 20.1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone) under all lighting conditions. Kinetics (to the time of attaining maximum levels in situ) of the increase under diurnal lighting and in constant darkness were indistinguishable from those in vivo. A high concentration of dl-propranolol markedly stimulated an increase in acetyltransferase activity in glands cultured in constant darkness but had little effect on glands under diurnal lighting or continuous illumination."}
{"id": "PMID:476516", "title": "Structural homologies in alanine-rich acidic ribosomal proteins from procaryotes and eucaryotes.", "content": "The amino acid composition and amino-terminal sequence have been determined for the alanine-rich, acidic ribosomal 'A' protein (equivalent to Escherichia coli L7/L12) from three procaryotic cell types that live under extreme environmental conditions (Arthrobacter glacialis, Clostridium pasteurianum, and Bacillus stearothermophilus) as well as from wheat germ, a eucaryote source. These data are compared with previously published 'A' protein sequences from other procaryotes and eucaryotes. All the procaryotic 'A' proteins, with the exception of the very acidic 'A' protein from Halobacterium cutirubrum, show similar charge, size, and amino acid composition, as well as an extensive sequence homology in the N-terminal region. Some differences are observed between gram-negative and gram-positive bacteria. The 'A' proteins from eucaryotes contain two tyrosine molecules, an amino acid absent in procaryotic 'A' proteins, as well as a reduced number of valine residues and an increased amount of aspartic acid. The N-terminal sequence of wheat germ 'A' protein shows considerable homology with other eucaryotic 'A' proteins and also with H. cutirubrum. It also shows some sequence homology with E. coli 'A' proteins.", "contents": "Structural homologies in alanine-rich acidic ribosomal proteins from procaryotes and eucaryotes. The amino acid composition and amino-terminal sequence have been determined for the alanine-rich, acidic ribosomal 'A' protein (equivalent to Escherichia coli L7/L12) from three procaryotic cell types that live under extreme environmental conditions (Arthrobacter glacialis, Clostridium pasteurianum, and Bacillus stearothermophilus) as well as from wheat germ, a eucaryote source. These data are compared with previously published 'A' protein sequences from other procaryotes and eucaryotes. All the procaryotic 'A' proteins, with the exception of the very acidic 'A' protein from Halobacterium cutirubrum, show similar charge, size, and amino acid composition, as well as an extensive sequence homology in the N-terminal region. Some differences are observed between gram-negative and gram-positive bacteria. The 'A' proteins from eucaryotes contain two tyrosine molecules, an amino acid absent in procaryotic 'A' proteins, as well as a reduced number of valine residues and an increased amount of aspartic acid. The N-terminal sequence of wheat germ 'A' protein shows considerable homology with other eucaryotic 'A' proteins and also with H. cutirubrum. It also shows some sequence homology with E. coli 'A' proteins."}
{"id": "PMID:476517", "title": "Electron microscopy of serum amyloid protein in the presence of calcium; alternative forms of assembly of pentagonal molecules in two-dimensional lattices.", "content": "The P component is a protein believed to be of serum origin commonly found in the highly ordered proteinaceous tissue deposits called amyloid. Both the protein recovered from the tissue and its serum counterpart have an identical characteristic appearance in the electron microscope, consisting of pentagonal plates which are often assembled as columns of stacked discs. These images, seen in the absence of calcium, are replaced by three more complex assemblies when low concentrations of calcium are present. One of the structures is crystalline (III), the other two appear to be planar lattices, one having a distinguishable structure with threefold symmetry (1). The structural elements of the other lattice (II) which takes the form of a cylinder are less distinct. It is suggested that the regular arrays which P component forms in the presence of calcium may be the basic framework on which amyloid deposits form.", "contents": "Electron microscopy of serum amyloid protein in the presence of calcium; alternative forms of assembly of pentagonal molecules in two-dimensional lattices. The P component is a protein believed to be of serum origin commonly found in the highly ordered proteinaceous tissue deposits called amyloid. Both the protein recovered from the tissue and its serum counterpart have an identical characteristic appearance in the electron microscope, consisting of pentagonal plates which are often assembled as columns of stacked discs. These images, seen in the absence of calcium, are replaced by three more complex assemblies when low concentrations of calcium are present. One of the structures is crystalline (III), the other two appear to be planar lattices, one having a distinguishable structure with threefold symmetry (1). The structural elements of the other lattice (II) which takes the form of a cylinder are less distinct. It is suggested that the regular arrays which P component forms in the presence of calcium may be the basic framework on which amyloid deposits form."}
{"id": "PMID:476518", "title": "The amino acid sequence of porcine intestinal calcium-binding protein.", "content": "The complete amino acid sequence of the calcium-binding protein (CaBP) from pig intestinal mucosa has been determined: Ac-Ser-Ala-Gln-Lys-Ser-Pro-Ala-Glu-Leu-Lys-Ser-Ile-Phe-Glu-Lys-Tyr-Ala-Ala-Lys-Glu-Gly-Asp-Pro-Asn-Gln-Leu-Ser-Lys-Glu-Glu-Leu-Lys-Gln-Leu-Ile-Gln-Ala-Glu-Phe-Pro-Ser-Leu-Leu-Lys-Gly-Pro-Arg-Thr-Leu-Asp-Asp-Leu-Phe-Gln-Glu-Leu-Asp-Lys-Asn-Gly-Asn-Gly-Glu-Val-Ser-Phe-Glu-Glu-Phe-Gln-Val-Leu-Val-Lys-Lys-Ile-Ser-Gln-OH. The N-terminal octapeptide sequence was determined by mass spectrometric analysis by Morris and Dell. The first 45 residues of bovine CaBP differ only in six positions from the corresponding sequence of the porcine protein, except that the sequence starts in position two of the porcine sequence. The mammalian intestinal CaBP's belong to the troponin-C superfamily on the basis of an analysis by Barker and Dayhoff.", "contents": "The amino acid sequence of porcine intestinal calcium-binding protein. The complete amino acid sequence of the calcium-binding protein (CaBP) from pig intestinal mucosa has been determined: Ac-Ser-Ala-Gln-Lys-Ser-Pro-Ala-Glu-Leu-Lys-Ser-Ile-Phe-Glu-Lys-Tyr-Ala-Ala-Lys-Glu-Gly-Asp-Pro-Asn-Gln-Leu-Ser-Lys-Glu-Glu-Leu-Lys-Gln-Leu-Ile-Gln-Ala-Glu-Phe-Pro-Ser-Leu-Leu-Lys-Gly-Pro-Arg-Thr-Leu-Asp-Asp-Leu-Phe-Gln-Glu-Leu-Asp-Lys-Asn-Gly-Asn-Gly-Glu-Val-Ser-Phe-Glu-Glu-Phe-Gln-Val-Leu-Val-Lys-Lys-Ile-Ser-Gln-OH. The N-terminal octapeptide sequence was determined by mass spectrometric analysis by Morris and Dell. The first 45 residues of bovine CaBP differ only in six positions from the corresponding sequence of the porcine protein, except that the sequence starts in position two of the porcine sequence. The mammalian intestinal CaBP's belong to the troponin-C superfamily on the basis of an analysis by Barker and Dayhoff."}
{"id": "PMID:476519", "title": "The interaction between beef cardiac troponin T and troponin I as demonstrated by ultraviolet absorption difference spectroscopy, circular dichroism, and gel filtration.", "content": "The specific interaction of bovine cardiac troponin T with troponin I has been demonstrated at a 1:1 molar ratio by absorption difference spectroscopy, near and far ultraviolet circular dichroism, and gel filtration chromatography. The maintenance of the sulfhydryl groups of both proteins in the reduced state was essential in order to demonstrate interaction between cardiac troponin I and troponin T using the aforementioned methodology. Carboxamido-methylated troponin I and troponin T samples were prepared by reaction with iodoacetamide. Spectrophotometric titration of the two proteins with 2-chloromercurinitrophenol and amino acid analysis of their carboxamidomethylated derivatives revealed that cardiac troponin I possesses two cysteine residues while cardiac troponin T has one. The modified troponin T possesses properties identical to those of the native molecule. The modification of troponin I is accompanied by an increase in secondary structure and a loss in ability to interact with troponin T at 0.5 M NaCl ionic strength. However, at 0.3 M NaCl the modified troponin I was shown by gel filtration chromoatography to interact very weakly with troponin T. On the other hand, the modified troponin I interacts with troponin C in a manner identical to the native protein, indicating that the troponin T interaction domain of the molecule is distinct from that region which interacts with troponin C.", "contents": "The interaction between beef cardiac troponin T and troponin I as demonstrated by ultraviolet absorption difference spectroscopy, circular dichroism, and gel filtration. The specific interaction of bovine cardiac troponin T with troponin I has been demonstrated at a 1:1 molar ratio by absorption difference spectroscopy, near and far ultraviolet circular dichroism, and gel filtration chromatography. The maintenance of the sulfhydryl groups of both proteins in the reduced state was essential in order to demonstrate interaction between cardiac troponin I and troponin T using the aforementioned methodology. Carboxamido-methylated troponin I and troponin T samples were prepared by reaction with iodoacetamide. Spectrophotometric titration of the two proteins with 2-chloromercurinitrophenol and amino acid analysis of their carboxamidomethylated derivatives revealed that cardiac troponin I possesses two cysteine residues while cardiac troponin T has one. The modified troponin T possesses properties identical to those of the native molecule. The modification of troponin I is accompanied by an increase in secondary structure and a loss in ability to interact with troponin T at 0.5 M NaCl ionic strength. However, at 0.3 M NaCl the modified troponin I was shown by gel filtration chromoatography to interact very weakly with troponin T. On the other hand, the modified troponin I interacts with troponin C in a manner identical to the native protein, indicating that the troponin T interaction domain of the molecule is distinct from that region which interacts with troponin C."}
{"id": "PMID:476520", "title": "Lysyl dipeptide and tripeptide model systems for racemization studies in amino acid and peptide chemistry.", "content": "Four series of diastereomeric peptide pairs (X-Lys, Lys-Y, Gly-X-Lys, and Gly-Lys-Y; where X and Y = Ala, Leu, Val, Ile, Phe, and Pro) have been synthesized by conventional procedures using benzyl-based protecting groups. Chromatographic separation of each pair, except Gly-Lys-Pro, has been achieved by elution from a 15-cm Aminex A-5 resin column using pH 5.5 buffer for tripeptides, pH 6.5 buffer for dipeptides, and pH 7.5 buffer for phenylalanyl peptides. The isomers have been identified by chromatography of the L-L isomers. Examples of the use of the model peptides for optical purity determinations and as tests for racemization are presented. The series Gly-X-Lys allows comparison of the tendencies to racemize of residues X during couplings. The separations of other peptides where Y = MeVal and X = N- and O-methylhydroxyamino acids are also described.", "contents": "Lysyl dipeptide and tripeptide model systems for racemization studies in amino acid and peptide chemistry. Four series of diastereomeric peptide pairs (X-Lys, Lys-Y, Gly-X-Lys, and Gly-Lys-Y; where X and Y = Ala, Leu, Val, Ile, Phe, and Pro) have been synthesized by conventional procedures using benzyl-based protecting groups. Chromatographic separation of each pair, except Gly-Lys-Pro, has been achieved by elution from a 15-cm Aminex A-5 resin column using pH 5.5 buffer for tripeptides, pH 6.5 buffer for dipeptides, and pH 7.5 buffer for phenylalanyl peptides. The isomers have been identified by chromatography of the L-L isomers. Examples of the use of the model peptides for optical purity determinations and as tests for racemization are presented. The series Gly-X-Lys allows comparison of the tendencies to racemize of residues X during couplings. The separations of other peptides where Y = MeVal and X = N- and O-methylhydroxyamino acids are also described."}
{"id": "PMID:476521", "title": "Lysozyme catalysis: kinetics of the hydrolysis of cell wall oligosaccharides.", "content": "The cleavage of cell wall tetrasaccharide, the beta(1 leads to 4)-linked dimer of the basic repeating disaccharide N-acetyl-D-glucosamine-beta(1 leads to 4)-N-acetyl-D-muramic acid, by lysozyme has been studied at various concentrations of lysozyme and over long time ranges. A theoretical analysis of the kinetic results indicates that direct hydrolysis of the tetrasaccharide by binding in subsities CDEF of the active site of lysozyme is significant at long times relative to the transglycosylation pathway. The binding constant for tetrasaccharide in CDEF is shown to be 10(3) times larger than that determined on the basis of an analysis of kinetic data over a more restricted range of times and concentrations.", "contents": "Lysozyme catalysis: kinetics of the hydrolysis of cell wall oligosaccharides. The cleavage of cell wall tetrasaccharide, the beta(1 leads to 4)-linked dimer of the basic repeating disaccharide N-acetyl-D-glucosamine-beta(1 leads to 4)-N-acetyl-D-muramic acid, by lysozyme has been studied at various concentrations of lysozyme and over long time ranges. A theoretical analysis of the kinetic results indicates that direct hydrolysis of the tetrasaccharide by binding in subsities CDEF of the active site of lysozyme is significant at long times relative to the transglycosylation pathway. The binding constant for tetrasaccharide in CDEF is shown to be 10(3) times larger than that determined on the basis of an analysis of kinetic data over a more restricted range of times and concentrations."}
{"id": "PMID:476523", "title": "Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase from porcine liver. Location of the activities in two domains of the multifunctional polypeptide.", "content": "Chymotryptic cleavage of the trifunctional protein methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase from pig liver yields a fragment of two-thirds the original polypeptide that retains only synthetase activity. A smaller polypeptide corresponding to about one-third of the original polypeptide was shown earlier to retain dehydrogenase-cyclohydrolase activity. On immunodiffusion, the synthetase fragment cross-reacts and shows partial identity with antibodies raised against the uncleaved enzyme but shows nonidentity with the dehydrogenase-cyclohydrolase fragment, suggesting that the two fragments are derived from different regions of the polypeptide. Amino-terminal analysis of the peptides and uncleaved enzyme indicate that the dehydrogenase-cyclohydrolase activities are located at the amino-terminal region and the synthetase near the carboxyl-terminal portion of the polypeptide.", "contents": "Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase from porcine liver. Location of the activities in two domains of the multifunctional polypeptide. Chymotryptic cleavage of the trifunctional protein methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase from pig liver yields a fragment of two-thirds the original polypeptide that retains only synthetase activity. A smaller polypeptide corresponding to about one-third of the original polypeptide was shown earlier to retain dehydrogenase-cyclohydrolase activity. On immunodiffusion, the synthetase fragment cross-reacts and shows partial identity with antibodies raised against the uncleaved enzyme but shows nonidentity with the dehydrogenase-cyclohydrolase fragment, suggesting that the two fragments are derived from different regions of the polypeptide. Amino-terminal analysis of the peptides and uncleaved enzyme indicate that the dehydrogenase-cyclohydrolase activities are located at the amino-terminal region and the synthetase near the carboxyl-terminal portion of the polypeptide."}
{"id": "PMID:476524", "title": "Relationships among purine nucleoside metabolism, adenosine triphosphate catabolism, and glycolysis in human erythrocytes.", "content": "In human erythrocytes incubated with both naturally occurring purine nucleosides and with a variety of purine nucleoside analogs, ATP catabolism was accelerated and lactate accumulation was increased. Tubercidin was a particularly potent inducer of ATP catabolism. In cells incubated with tubercidin, the major route of adenylate metabolism was deamination, whereas in cells incubated with deoxyglucose, the major pathway was dephosphorylation.", "contents": "Relationships among purine nucleoside metabolism, adenosine triphosphate catabolism, and glycolysis in human erythrocytes. In human erythrocytes incubated with both naturally occurring purine nucleosides and with a variety of purine nucleoside analogs, ATP catabolism was accelerated and lactate accumulation was increased. Tubercidin was a particularly potent inducer of ATP catabolism. In cells incubated with tubercidin, the major route of adenylate metabolism was deamination, whereas in cells incubated with deoxyglucose, the major pathway was dephosphorylation."}
{"id": "PMID:476525", "title": "The ribosomal RNA of the trypanosomatid protozoan Crithidia fasciculata: physical characteristics and methylated sequences.", "content": "When extracted and analyzed under conditions which maintain noncovalently associated RNA-RNA complexes, the bulk cellular RNA of Crithidia fasciculata contains species of apparent molecular weights 1.3, 0.825, 0.08, 0.065, and 0.045 x 10(6) in addition to 5S rRNA and tRNA. Heat denaturation results in the disappearance of the 1.3 x 10(6) dalton RNA and the appearance of three new species having molecular weights of 0.67, 0.575, and 0.059 x 10(6). In addition, the apparent molecular weight of the 0.825 x 10(6) dalton component is reproducibly lowered to 0.81 x 10(6) after heat treatment. With the exception of tRNA, all of the RNA species are present in close to equimolar amounts in either undenatured or heat-denatured C. fasciculata bulk cellular RNA. On the basis of previous observations on the ribosomal RNA of the closely related organism, Crithidia oncopelti (Spencer, R. & Cross, G.A.M. (1976) J. Gen. Microbiol. 93, 82-88), the 1.3 and 0.825 x 10(6) dalton RNA's are considered to be components of the large and small subunits, respectively, of C. fasciculata ribosomes, but the subunit localization of the other RNA's described here has not yet been determined. O2'-Methylnucleosides account for about 1.4 mol% of the total nucleoside constituents of unfractionated C. fasciculata rRNA. Quantitative analysis suggests that the rRNA molecules in a C. fasciculata ribosome contain a total of 95-100 O2'-methyl groups, distributed in 80-85 Nm-Np sequences (including four 'hypermodified' Nm-Np, each containing a modification of a base or base-sugar linkage in addition to sugar methylation), six different Nm-Nm-Np sequences, and one Nm-Nm-Nm-Np sequence. While the specific pattern of O2'-methylation in the rRNA of C. fasciculata is distinct, both qualitatively and quantitatively, from the pattern observed in other organisms, Crithidia rRNA does contain certain 'universal' O2'-methylated sequences which appear to have been extensively conserved in evolution. The base-methylated nucleoside, N6,N6-dimethyladenosine (m26A), has been isolated from both C. fasciculata and wheat embryo rRNA in the form of the alkali-resistant dinucleotide, m26A-m26Ap. This dinucleotide and its enzymatic degradation products have been characterized by examination of their ultraviolet absorption spectra and electrophoretic and chromatographic properties.", "contents": "The ribosomal RNA of the trypanosomatid protozoan Crithidia fasciculata: physical characteristics and methylated sequences. When extracted and analyzed under conditions which maintain noncovalently associated RNA-RNA complexes, the bulk cellular RNA of Crithidia fasciculata contains species of apparent molecular weights 1.3, 0.825, 0.08, 0.065, and 0.045 x 10(6) in addition to 5S rRNA and tRNA. Heat denaturation results in the disappearance of the 1.3 x 10(6) dalton RNA and the appearance of three new species having molecular weights of 0.67, 0.575, and 0.059 x 10(6). In addition, the apparent molecular weight of the 0.825 x 10(6) dalton component is reproducibly lowered to 0.81 x 10(6) after heat treatment. With the exception of tRNA, all of the RNA species are present in close to equimolar amounts in either undenatured or heat-denatured C. fasciculata bulk cellular RNA. On the basis of previous observations on the ribosomal RNA of the closely related organism, Crithidia oncopelti (Spencer, R. & Cross, G.A.M. (1976) J. Gen. Microbiol. 93, 82-88), the 1.3 and 0.825 x 10(6) dalton RNA's are considered to be components of the large and small subunits, respectively, of C. fasciculata ribosomes, but the subunit localization of the other RNA's described here has not yet been determined. O2'-Methylnucleosides account for about 1.4 mol% of the total nucleoside constituents of unfractionated C. fasciculata rRNA. Quantitative analysis suggests that the rRNA molecules in a C. fasciculata ribosome contain a total of 95-100 O2'-methyl groups, distributed in 80-85 Nm-Np sequences (including four 'hypermodified' Nm-Np, each containing a modification of a base or base-sugar linkage in addition to sugar methylation), six different Nm-Nm-Np sequences, and one Nm-Nm-Nm-Np sequence. While the specific pattern of O2'-methylation in the rRNA of C. fasciculata is distinct, both qualitatively and quantitatively, from the pattern observed in other organisms, Crithidia rRNA does contain certain 'universal' O2'-methylated sequences which appear to have been extensively conserved in evolution. The base-methylated nucleoside, N6,N6-dimethyladenosine (m26A), has been isolated from both C. fasciculata and wheat embryo rRNA in the form of the alkali-resistant dinucleotide, m26A-m26Ap. This dinucleotide and its enzymatic degradation products have been characterized by examination of their ultraviolet absorption spectra and electrophoretic and chromatographic properties."}
{"id": "PMID:476528", "title": "Protamine messenger RNA: partial purification and characterization of a heterogeneous family of polyadenylated messenger components.", "content": "Poly(A)+ protamine mRNA (pmRNA) components were isolated after separation on denaturing preparative polyacrylamide gels. The four size classes of protamine mRNA described previously were found to contain poly(A) tracts of different lengths. The pmRNA1 was found to be associated with (A)110, pmRNA2 with (A)90, pmRNA3 with (A)85, and pmRNA4 with (A)69. Following deadenylation with RNase H after duplex formation with oligo-dT, the isolated mRNAs were found to be still heterogeneous, although highly enriched in certain of the deadenylated components. DNA complementary to the isolated mRNAs (cDNA) was synthesized in vitro. Following depurination, the oligopyrimidine maps indicated that C7T4, corresponding to an Arg-Arg-Gly-Gly sequence in protamine and originally thought to be characteristic of all mRNA components, is present in only one or possibly tow of the components. Cross-hybridizations between the cDNAs and the four poly(A)+ pmRNAs indicated that a basic polynucleotide unit of substantial length is common to all four mRNAs and that the existing nucleotide sequence variations probably originate from one or both of the non-coding portions of the mRNA molecules.", "contents": "Protamine messenger RNA: partial purification and characterization of a heterogeneous family of polyadenylated messenger components. Poly(A)+ protamine mRNA (pmRNA) components were isolated after separation on denaturing preparative polyacrylamide gels. The four size classes of protamine mRNA described previously were found to contain poly(A) tracts of different lengths. The pmRNA1 was found to be associated with (A)110, pmRNA2 with (A)90, pmRNA3 with (A)85, and pmRNA4 with (A)69. Following deadenylation with RNase H after duplex formation with oligo-dT, the isolated mRNAs were found to be still heterogeneous, although highly enriched in certain of the deadenylated components. DNA complementary to the isolated mRNAs (cDNA) was synthesized in vitro. Following depurination, the oligopyrimidine maps indicated that C7T4, corresponding to an Arg-Arg-Gly-Gly sequence in protamine and originally thought to be characteristic of all mRNA components, is present in only one or possibly tow of the components. Cross-hybridizations between the cDNAs and the four poly(A)+ pmRNAs indicated that a basic polynucleotide unit of substantial length is common to all four mRNAs and that the existing nucleotide sequence variations probably originate from one or both of the non-coding portions of the mRNA molecules."}
{"id": "PMID:476530", "title": "Conservation of nucleolus organizer regions during evolution in sheep, goat, cattle and aoudad.", "content": "There are ten nucleolus organizer regions (NORs) in domestic sheep (Ovis aries L.). cattle (Bos taurus L.), goat (Capra hircus L.) and aoudad (Ammotragus lervia Blyth) and these are located terminally on chromosomes with homologous (G-banding patterns. The similarity in number of nucleolus organizer regions in these species may indicate that their ribosomal DNA regions are infrequently involved in exchange events which could lead to different numbers of active nucleolus organizer regions. Other possible explanations of the conservation of number of nucleolus organizer regions in these species are discussed. The homology of NOR location in these species supports the idea that the Bovidae karyotype tends to be fairly stable apart from changes due to centric fusion events.", "contents": "Conservation of nucleolus organizer regions during evolution in sheep, goat, cattle and aoudad. There are ten nucleolus organizer regions (NORs) in domestic sheep (Ovis aries L.). cattle (Bos taurus L.), goat (Capra hircus L.) and aoudad (Ammotragus lervia Blyth) and these are located terminally on chromosomes with homologous (G-banding patterns. The similarity in number of nucleolus organizer regions in these species may indicate that their ribosomal DNA regions are infrequently involved in exchange events which could lead to different numbers of active nucleolus organizer regions. Other possible explanations of the conservation of number of nucleolus organizer regions in these species are discussed. The homology of NOR location in these species supports the idea that the Bovidae karyotype tends to be fairly stable apart from changes due to centric fusion events."}
{"id": "PMID:476531", "title": "The use of the Salmonella/microsomal assay to determine mutagenicity in paired chemical mixtures.", "content": "The mutagenicities of two sets of chemicals acting singly and in pairwise combinations were determined by use of the Salmonella/microsomal assay. The first set consisted of the promutagens of benzo(a)pyrene and benzo(rst)pentaphene. The second set contained the direct-acting mutagens methyl-nitro-nitroso-guanidine and ethyl methane sulfonate. In the tests with the promutagens, the quantities of S-9 mix were varied over the range of 0.05 ml to 1.0 ml with increasing quantities of each chemical. The mutagenic responses or production of revertant colonies of the promutagens, acting singly and in pairwise combinations failed to show an additive effect. Excess quantities of S-9 mix appeared to inhibit partially or totally the mutagenic activity of each chemical, although for each particular dose there was an optimal quantity of S-9 mix to induce maximum activity. However, the direct-acting mutagens produced, individually, almost linear dose responses with increasing concentrations. In pairwise combinations, these chemicals also showed linear responses that closely approximated the theoretical additivity indicating that the mutagenicity of the mixtures was the sum of the activities of each component.", "contents": "The use of the Salmonella/microsomal assay to determine mutagenicity in paired chemical mixtures. The mutagenicities of two sets of chemicals acting singly and in pairwise combinations were determined by use of the Salmonella/microsomal assay. The first set consisted of the promutagens of benzo(a)pyrene and benzo(rst)pentaphene. The second set contained the direct-acting mutagens methyl-nitro-nitroso-guanidine and ethyl methane sulfonate. In the tests with the promutagens, the quantities of S-9 mix were varied over the range of 0.05 ml to 1.0 ml with increasing quantities of each chemical. The mutagenic responses or production of revertant colonies of the promutagens, acting singly and in pairwise combinations failed to show an additive effect. Excess quantities of S-9 mix appeared to inhibit partially or totally the mutagenic activity of each chemical, although for each particular dose there was an optimal quantity of S-9 mix to induce maximum activity. However, the direct-acting mutagens produced, individually, almost linear dose responses with increasing concentrations. In pairwise combinations, these chemicals also showed linear responses that closely approximated the theoretical additivity indicating that the mutagenicity of the mixtures was the sum of the activities of each component."}
{"id": "PMID:476532", "title": "Frequencies of centromeric heteromorphisms of human chromosomes 3 and 4 as detected by QFQ technique: can they be identified by RFA technique?", "content": "One hundred normal Caucasians were studied by sequential QFQ and RFA banding techniques in order to estimate the type and frequency of heteromorphisms in the centromeric regions of chromosome 3 and 4. Intensity variants were classified into 1 of 5 levels of QFQ banding. QFQ intensity heteromorphisms (greater than or equal to level 3) for chromosomes 3 and 4 were 62 and 15 percent respectively. The interrelationship between QFQ and RFA variants were also examined. When the centromere was brilliant by QFQ, it was found that it was deep red by RFA; when it was pale by QFQ, it was light red by RFA. Neverthless, a blind coded study could not pick up these color variants by RFA. QFQ banding showed variations of the centromeric regions of chromosomes 3 and 4 while RFA banding failed to demonstrate it. It was concluded that QFQ  is the most useful technique in detecting the different intensity levels in the centromeric regions of chromsomes 3 and 4.", "contents": "Frequencies of centromeric heteromorphisms of human chromosomes 3 and 4 as detected by QFQ technique: can they be identified by RFA technique? One hundred normal Caucasians were studied by sequential QFQ and RFA banding techniques in order to estimate the type and frequency of heteromorphisms in the centromeric regions of chromosome 3 and 4. Intensity variants were classified into 1 of 5 levels of QFQ banding. QFQ intensity heteromorphisms (greater than or equal to level 3) for chromosomes 3 and 4 were 62 and 15 percent respectively. The interrelationship between QFQ and RFA variants were also examined. When the centromere was brilliant by QFQ, it was found that it was deep red by RFA; when it was pale by QFQ, it was light red by RFA. Neverthless, a blind coded study could not pick up these color variants by RFA. QFQ banding showed variations of the centromeric regions of chromosomes 3 and 4 while RFA banding failed to demonstrate it. It was concluded that QFQ  is the most useful technique in detecting the different intensity levels in the centromeric regions of chromsomes 3 and 4."}
{"id": "PMID:476533", "title": "Sperm size abnormalities in homozygous and heterozygous In(5)9Rk mice.", "content": "Sperm abnormalities were scored in In(5)9Rk homozygotes, heterozygotes and in the inbred strains, C57BL/6J, C3H/HeJ, DBA/2J and JUFaCt, used to produce the geterozygotes. The sperm abnormalities of the inversion heterozygotes were remarkable, about 40% were either double-headed or abnormally large in size. The double-headed sperm had head dimensions similar to those of normal-sized sperm, but the large sperm heads were 1.2 times longer and 1.3 times wider and presumably had twice the usual nuclear volume. This observation suggests that the anaphase bridge formed in inversion heterozygotes resulting from the paracentric inversion may constitute an impediment to cytokinesis and may lead to two genomes being packaged into a single sperm. If this is a general phenomenon, sperm morphology may provide a useful screen for paracentric inversions.", "contents": "Sperm size abnormalities in homozygous and heterozygous In(5)9Rk mice. Sperm abnormalities were scored in In(5)9Rk homozygotes, heterozygotes and in the inbred strains, C57BL/6J, C3H/HeJ, DBA/2J and JUFaCt, used to produce the geterozygotes. The sperm abnormalities of the inversion heterozygotes were remarkable, about 40% were either double-headed or abnormally large in size. The double-headed sperm had head dimensions similar to those of normal-sized sperm, but the large sperm heads were 1.2 times longer and 1.3 times wider and presumably had twice the usual nuclear volume. This observation suggests that the anaphase bridge formed in inversion heterozygotes resulting from the paracentric inversion may constitute an impediment to cytokinesis and may lead to two genomes being packaged into a single sperm. If this is a general phenomenon, sperm morphology may provide a useful screen for paracentric inversions."}
{"id": "PMID:476534", "title": "Considerations of a method of analyzing diallel crosses of atlantic salmon.", "content": "Inferences derived from a proposed mixed model analysis of a diallel cross involving four stocks of Atlantic salmon (Salmo salar) are illustrated with growth data on weight and length. Variation between stocks was more apparent when samples represented dams as opposed to sires, thus stressing the relative importance of maternal effects. However, the ranking of stocks was not altered when either the means of sire sources or the means of dam sources were considered. No heterotic effects were found for the growth traits studied.", "contents": "Considerations of a method of analyzing diallel crosses of atlantic salmon. Inferences derived from a proposed mixed model analysis of a diallel cross involving four stocks of Atlantic salmon (Salmo salar) are illustrated with growth data on weight and length. Variation between stocks was more apparent when samples represented dams as opposed to sires, thus stressing the relative importance of maternal effects. However, the ranking of stocks was not altered when either the means of sire sources or the means of dam sources were considered. No heterotic effects were found for the growth traits studied."}
{"id": "PMID:476535", "title": "The chromosomes of the Canadian Beaver Castor canadensis.", "content": "A chromosome analysis of 24 Canadian beavers, Castor canadensis Kuhl (12 males and 12 females), captured in Laurentides Park, Q\u00e9bec, has been performed from preparations of blood lymphocyte and skin cultures. The chromosome number was found to be 2n = 40. Measurements were made to determine relative lengths and arm ratios of chromosomes, which are metacentric or submetacentric. Results are in agreement with those already published regarding the chromosome number, but differ in the identification of the X chromosome, and in the morphology of the Y and some autosomes. C- ad G-banding techniques allowed the precise identification of individual chromosome pairs. A detailed idiogram of G-bands is presented.", "contents": "The chromosomes of the Canadian Beaver Castor canadensis. A chromosome analysis of 24 Canadian beavers, Castor canadensis Kuhl (12 males and 12 females), captured in Laurentides Park, Q\u00e9bec, has been performed from preparations of blood lymphocyte and skin cultures. The chromosome number was found to be 2n = 40. Measurements were made to determine relative lengths and arm ratios of chromosomes, which are metacentric or submetacentric. Results are in agreement with those already published regarding the chromosome number, but differ in the identification of the X chromosome, and in the morphology of the Y and some autosomes. C- ad G-banding techniques allowed the precise identification of individual chromosome pairs. A detailed idiogram of G-bands is presented."}
{"id": "PMID:476536", "title": "Direct genetic and postnatal maternal genetic effects on body composition in mice selected for body weight.", "content": "Line crossfostering techniques were used to study differences among selected and control lines of mice in direct genetic and postnatal maternal genetic influences on preweaning (day 12) body weight and composition. The lines were selected for high (H6) and low (L6) 6-week body weight and the control line (C2) was maintained by random selection. There were positive correlated responses to selection in both direct genetic and postnatal maternal genetic effects on body weight and weights of all body components (P less than 0.01) except for water and ash weight in H6. The correlated responses in postnatal maternal genetic effects were of the same order of magnitude as those in direct genetic effects. Correlated responses were greater in L6 than in H6. Correlated responses in direct genetic effects were positive (P less than 0.01) for water percent in H6 and ether extract percent in L6, and negative (P less than 0.01) for water percent and lean percent in L6. Correlated responses in postnatal maternal genetic effects were positive for ether extract percent and negative for water percent (P less than 0.01). Correlated responses were far greater in L6 than in H6 and were greater for postnatal maternal genetic effects than for direct genetic effects. Analyses of covariance results indicated line differences in the relative growth rates of the body components.", "contents": "Direct genetic and postnatal maternal genetic effects on body composition in mice selected for body weight. Line crossfostering techniques were used to study differences among selected and control lines of mice in direct genetic and postnatal maternal genetic influences on preweaning (day 12) body weight and composition. The lines were selected for high (H6) and low (L6) 6-week body weight and the control line (C2) was maintained by random selection. There were positive correlated responses to selection in both direct genetic and postnatal maternal genetic effects on body weight and weights of all body components (P less than 0.01) except for water and ash weight in H6. The correlated responses in postnatal maternal genetic effects were of the same order of magnitude as those in direct genetic effects. Correlated responses were greater in L6 than in H6. Correlated responses in direct genetic effects were positive (P less than 0.01) for water percent in H6 and ether extract percent in L6, and negative (P less than 0.01) for water percent and lean percent in L6. Correlated responses in postnatal maternal genetic effects were positive for ether extract percent and negative for water percent (P less than 0.01). Correlated responses were far greater in L6 than in H6 and were greater for postnatal maternal genetic effects than for direct genetic effects. Analyses of covariance results indicated line differences in the relative growth rates of the body components."}
{"id": "PMID:476537", "title": "Electrophoretic variation and differentiation in four strains of domesticated rainbow trout (Salmo gairdneri).", "content": "A total of 1462 rainbow trout (Salmo gairdneri Richardson) representing four California domestic strains, were examined electrophoretically at 24 gene loci to investigate the amount of genetic variability within strains and genetic differentiation between strains, relative to wild populations. Estimated heterozygosities of 0.071 to 0.134 were similar to those reported for wild populations and thus indicated that the domestic strains were at least as variable as wild populations. Genetic distances between strains ranged from 0.011 to 0.067, values typical of the level of differentiation observed between local conspecific populations in a variety of organisms. It was suggested that this high level of heterozygosity is due to mixing of populations in the strains' histories and perhaps also to balancing selection.", "contents": "Electrophoretic variation and differentiation in four strains of domesticated rainbow trout (Salmo gairdneri). A total of 1462 rainbow trout (Salmo gairdneri Richardson) representing four California domestic strains, were examined electrophoretically at 24 gene loci to investigate the amount of genetic variability within strains and genetic differentiation between strains, relative to wild populations. Estimated heterozygosities of 0.071 to 0.134 were similar to those reported for wild populations and thus indicated that the domestic strains were at least as variable as wild populations. Genetic distances between strains ranged from 0.011 to 0.067, values typical of the level of differentiation observed between local conspecific populations in a variety of organisms. It was suggested that this high level of heterozygosity is due to mixing of populations in the strains' histories and perhaps also to balancing selection."}
{"id": "PMID:476538", "title": "Can mutation theories of carcinogenesis set priorities for carcinogen testing programs?", "content": "The recent activity in designing, validating and implementing short-term tests for carcinogens has been spurred by the fairly convincing correlation between the carcinogenicity and mutagenicity of chemicals and by the assumption that mutations are somehow involved in neoplastic transformation. Moreover, it has been tacitly assumed that the mutagenic capacity alone of compounds would induce regulatory agencies to pass rules for their removal from man's environment, and would lead the public to avoid them. The actual response, however, is quite different. Government departments shy away from making any decisions on the basis of in vitro test systems, the public at large is becoming irritated by daily announcements that many of their cherished habits could adversely affect their health, and industries feel threatened and may reduce their search for new beneficial chemicals. The reluctance to accept wholeheartedly the mutagenicity tests for the detection of carcinogens is partly due to the uncertainty about the involvement of mutations in the formation of benign and malignant tumors. Following the initial rapid advances in the detection of environmental chemicals with carcinogenic and mutagenic properties, we seem to have arrived at the cross roads: we must now set new priorities for future research, and must make an unbiased assessment of the actual hazard of a compound to man and the human population.", "contents": "Can mutation theories of carcinogenesis set priorities for carcinogen testing programs? The recent activity in designing, validating and implementing short-term tests for carcinogens has been spurred by the fairly convincing correlation between the carcinogenicity and mutagenicity of chemicals and by the assumption that mutations are somehow involved in neoplastic transformation. Moreover, it has been tacitly assumed that the mutagenic capacity alone of compounds would induce regulatory agencies to pass rules for their removal from man's environment, and would lead the public to avoid them. The actual response, however, is quite different. Government departments shy away from making any decisions on the basis of in vitro test systems, the public at large is becoming irritated by daily announcements that many of their cherished habits could adversely affect their health, and industries feel threatened and may reduce their search for new beneficial chemicals. The reluctance to accept wholeheartedly the mutagenicity tests for the detection of carcinogens is partly due to the uncertainty about the involvement of mutations in the formation of benign and malignant tumors. Following the initial rapid advances in the detection of environmental chemicals with carcinogenic and mutagenic properties, we seem to have arrived at the cross roads: we must now set new priorities for future research, and must make an unbiased assessment of the actual hazard of a compound to man and the human population."}
{"id": "PMID:476539", "title": "Structure of nitrogen-fixing nodules formed by Rhizobium on roots of Parasponia andersonii Planch.", "content": "The structure of nitrogen-fixing nodules produced by Rhizobium infection of the non-legume Parasponia andersonii was examined by light and electron (both SEM and TEM) microscopy. Comparisons were made with the nodules previously described on P. rugosa. Like the nodules on different non-legumes formed by other types of endophytes, the Rhizobium nodules on Parasponia resembled modified roots by having a central vascular bundle surrounded by an endophyte-infected zone. The intimate association between the Rhizobium and the host nodule cell was compared with the Rhizobium association found in legumes. The rhizobia were not released from the infection thread as happens in the legume. The infection thread, which propagates the Rhizobium infection to new cells, was transformed within a nodule cell from a darkly stained (light microscopy) or very electron-dense (TEM) structure to a number of thread types. The walls of the threads varied greatly in thickness and often the thread structures were without rigid walls and were only enclosed by a plasma membrane. If the rhizobia are transformed into bacteroids, as in the legumes, it would have to occur when the threads had reached their mature size, when bacterial division had ceased. Nitrogen fixation was considered to occur in all thread types.", "contents": "Structure of nitrogen-fixing nodules formed by Rhizobium on roots of Parasponia andersonii Planch. The structure of nitrogen-fixing nodules produced by Rhizobium infection of the non-legume Parasponia andersonii was examined by light and electron (both SEM and TEM) microscopy. Comparisons were made with the nodules previously described on P. rugosa. Like the nodules on different non-legumes formed by other types of endophytes, the Rhizobium nodules on Parasponia resembled modified roots by having a central vascular bundle surrounded by an endophyte-infected zone. The intimate association between the Rhizobium and the host nodule cell was compared with the Rhizobium association found in legumes. The rhizobia were not released from the infection thread as happens in the legume. The infection thread, which propagates the Rhizobium infection to new cells, was transformed within a nodule cell from a darkly stained (light microscopy) or very electron-dense (TEM) structure to a number of thread types. The walls of the threads varied greatly in thickness and often the thread structures were without rigid walls and were only enclosed by a plasma membrane. If the rhizobia are transformed into bacteroids, as in the legumes, it would have to occur when the threads had reached their mature size, when bacterial division had ceased. Nitrogen fixation was considered to occur in all thread types."}
{"id": "PMID:476540", "title": "Deoxyribonucleic acid relationships among members of the genus Aeromonas.", "content": "Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains. Large variations in genome size were found and % GC ranged from 57.1 to 62.9%. On the basis of the strains examined, the Genus Aeromonas consists of two genotypically legitimate groups: a diverse group of motile aeromonads, and the genetically more homogeneous non-motile aeromonads, comprising the species Aeromonas salmonicida. Internal homology groups could not be demonstrated within the motile aeromonads, and significant divergence in related sequences was indicated. This diverse motile group forms the single species Aeromonas hydrophila.", "contents": "Deoxyribonucleic acid relationships among members of the genus Aeromonas. Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains. Large variations in genome size were found and % GC ranged from 57.1 to 62.9%. On the basis of the strains examined, the Genus Aeromonas consists of two genotypically legitimate groups: a diverse group of motile aeromonads, and the genetically more homogeneous non-motile aeromonads, comprising the species Aeromonas salmonicida. Internal homology groups could not be demonstrated within the motile aeromonads, and significant divergence in related sequences was indicated. This diverse motile group forms the single species Aeromonas hydrophila."}
{"id": "PMID:476541", "title": "Effect of glycerol on viability and other properties of starved Mycobacterium fortuitum.", "content": "Cells of Mycobacterium fortuitum kept in 0.85% saline solution containing 0.1% Tween 80 (without glycerol) survive for a long time. In glycerol-enriched medium, they continue to lose their viability at a high rate; after 71 days of exposure the percentage of survival as indicated by colony formation and respiratory and dehydrogenase activities is lower than 1%. Surviving cells starved in medium without glycerol revealed unchanged sensitivity to streptomycin, p-aminosalicylic acid, and isoniazid.", "contents": "Effect of glycerol on viability and other properties of starved Mycobacterium fortuitum. Cells of Mycobacterium fortuitum kept in 0.85% saline solution containing 0.1% Tween 80 (without glycerol) survive for a long time. In glycerol-enriched medium, they continue to lose their viability at a high rate; after 71 days of exposure the percentage of survival as indicated by colony formation and respiratory and dehydrogenase activities is lower than 1%. Surviving cells starved in medium without glycerol revealed unchanged sensitivity to streptomycin, p-aminosalicylic acid, and isoniazid."}
{"id": "PMID:476542", "title": "Mannose transport in Amphora coffeaeformis var. perpusilla (Bacillariophyceae).", "content": "Amphora coffeaeformis var. perpusilla is able to concentrate but not metabolize mannose. The sugar, which can be accumulated against a concentration gradient, is taken up only after a short lag. Preincubation with glucose allows immediate mannose uptake. Substances that reduce the ATP content of the diatom cells also inhibit mannose uptake as do sugars of similar stereochemical configuration. Mannose appears to act as a non-metabolizable analogue of glucose in these respects.", "contents": "Mannose transport in Amphora coffeaeformis var. perpusilla (Bacillariophyceae). Amphora coffeaeformis var. perpusilla is able to concentrate but not metabolize mannose. The sugar, which can be accumulated against a concentration gradient, is taken up only after a short lag. Preincubation with glucose allows immediate mannose uptake. Substances that reduce the ATP content of the diatom cells also inhibit mannose uptake as do sugars of similar stereochemical configuration. Mannose appears to act as a non-metabolizable analogue of glucose in these respects."}
{"id": "PMID:476543", "title": "Effect of thiols on macroconidia of Fusarium sulphureum.", "content": "Treatment of Fusarium sulphureum macroconidial cells with five thiols alters their morphology. Macroconidial cells incubated in dithiothreitol (DTT), dithioerythritol (DTE), or thiourea differentiate into thick-walled, chlamydospore-like cells (thiol-induced spores). These cells appear similar in size and shape to chlamydospores in the light microscope, but differ markedly in cell wall structure when viewed in the electron microscope (EM). Incubation of macroconidia with both DTT and DTE also leads to the formation of large swollen cells (giant cells) which have a parietal cytoplasm and electron-tranparent cell walls; most of these giant cells lyse within 3 to 7 days of incubation. Thiourea-induced spores are characterized by the deposition of a thick, electron-dense, extracellular layer and an accumulation of mitochondria. DTT and DTE, at the concentrations used, inhibit macroconidial germination while thiourea, mercaptoethanol, and cysteine do not. With the latter three thiols, the newly formed hyphal cells become elongated with either one or both ends swollen. Mercaptoethanol-treated cells contain an abundance of mitochondria. The DTT-induced spore differs from both macroconidia and chlamydospores with respect to cellular lipid and cell wall composition. While the thiols have different effects on the macroconidia, the fact that they all induce cell expansion suggests that they react at some common sites.", "contents": "Effect of thiols on macroconidia of Fusarium sulphureum. Treatment of Fusarium sulphureum macroconidial cells with five thiols alters their morphology. Macroconidial cells incubated in dithiothreitol (DTT), dithioerythritol (DTE), or thiourea differentiate into thick-walled, chlamydospore-like cells (thiol-induced spores). These cells appear similar in size and shape to chlamydospores in the light microscope, but differ markedly in cell wall structure when viewed in the electron microscope (EM). Incubation of macroconidia with both DTT and DTE also leads to the formation of large swollen cells (giant cells) which have a parietal cytoplasm and electron-tranparent cell walls; most of these giant cells lyse within 3 to 7 days of incubation. Thiourea-induced spores are characterized by the deposition of a thick, electron-dense, extracellular layer and an accumulation of mitochondria. DTT and DTE, at the concentrations used, inhibit macroconidial germination while thiourea, mercaptoethanol, and cysteine do not. With the latter three thiols, the newly formed hyphal cells become elongated with either one or both ends swollen. Mercaptoethanol-treated cells contain an abundance of mitochondria. The DTT-induced spore differs from both macroconidia and chlamydospores with respect to cellular lipid and cell wall composition. While the thiols have different effects on the macroconidia, the fact that they all induce cell expansion suggests that they react at some common sites."}
{"id": "PMID:476544", "title": "[Effect of the saccharide chain length of bacterial lipopolysacchrides on the stimulation of immune system].", "content": "Three lipopolysaccharides (LPS) of different chain length were used as adjuvant. The one of shortest chain length is the most effective in enhancing antibody titer in rats against sheep red blood cells, suggesting that lipid A and KDO were responsible for the stimulatory effect of LPS.", "contents": "[Effect of the saccharide chain length of bacterial lipopolysacchrides on the stimulation of immune system]. Three lipopolysaccharides (LPS) of different chain length were used as adjuvant. The one of shortest chain length is the most effective in enhancing antibody titer in rats against sheep red blood cells, suggesting that lipid A and KDO were responsible for the stimulatory effect of LPS."}
{"id": "PMID:476545", "title": "A dominant temperature-sensitive assembly mutant of adenovirus type 2.", "content": "An assembly negative temperature-sensitive mutant of Ad2, ts48 was shown to exert dominance over other ts mutants and wild-type virus during coinfection, by inhibiting virion assembly. Dominance was only expressed at the nonpermissive temperature.", "contents": "A dominant temperature-sensitive assembly mutant of adenovirus type 2. An assembly negative temperature-sensitive mutant of Ad2, ts48 was shown to exert dominance over other ts mutants and wild-type virus during coinfection, by inhibiting virion assembly. Dominance was only expressed at the nonpermissive temperature."}
{"id": "PMID:476546", "title": "Immunologic evidence that staphylococcal alpha toxin is oriented on membranes.", "content": "Antibodies to staphylococcal alpha toxin were separated into two distinct populations. One population prevented binding of alpha toxin onto erythrocyte membranes. The other population neutralized after the toxin was bound onto erythrocytes and thereby brought about an indirect hemagglutination reaction. The data suggest that alpha toxin has a membrane-binding region.", "contents": "Immunologic evidence that staphylococcal alpha toxin is oriented on membranes. Antibodies to staphylococcal alpha toxin were separated into two distinct populations. One population prevented binding of alpha toxin onto erythrocyte membranes. The other population neutralized after the toxin was bound onto erythrocytes and thereby brought about an indirect hemagglutination reaction. The data suggest that alpha toxin has a membrane-binding region."}
{"id": "PMID:476547", "title": "Isolation of Azospirullum from diverse geographic regions.", "content": "We have isolated Azospirillum (Spirullum lipoferum) from roots of grasses of several genera collected from a number of tropical and subtropical-temperate locations. Pure cultures were obtained from a small percentage of samples; no higher percentage was secured from tropical than from other grasses. Acetylene reduction and distinctive growth in N-free soft agar deeps were inadequate to identify this genus, although helpful in initial screening. Fluorescent antibody tests with antiserum against characterized strains were helpful. There is some evidence that this genus of bacteria may be favored in the rhizoplane.", "contents": "Isolation of Azospirullum from diverse geographic regions. We have isolated Azospirillum (Spirullum lipoferum) from roots of grasses of several genera collected from a number of tropical and subtropical-temperate locations. Pure cultures were obtained from a small percentage of samples; no higher percentage was secured from tropical than from other grasses. Acetylene reduction and distinctive growth in N-free soft agar deeps were inadequate to identify this genus, although helpful in initial screening. Fluorescent antibody tests with antiserum against characterized strains were helpful. There is some evidence that this genus of bacteria may be favored in the rhizoplane."}
{"id": "PMID:476548", "title": "[Study of a new group of Enterobacteriacea (group H1) related to Enterobacter cloacae strain].", "content": "A DNA-DNA hybridization study was carried out on a new group of enterobacteria (group H1) previously studied by numerical taxonomy work on the genus Enterobacter. This group showed a very high genetic homogeneity since the average relative binding ratio of nine analysed strains is equal to 91%. The taxonomic position of this group into the family of enterobacteria is discussed with the species E. cloacae (37 to 61%), K. pneumoniae (44 to 60%), K. oxytoca (57-58%), L. malonatica (syn. Citrobacter intermedius, a:46 to 54%), L. amalonatica (syn. C. intermedius, b: 51%), and the group H3 (52-61%). The group H1 is defined on phenotypic and genetic data.", "contents": "[Study of a new group of Enterobacteriacea (group H1) related to Enterobacter cloacae strain]. A DNA-DNA hybridization study was carried out on a new group of enterobacteria (group H1) previously studied by numerical taxonomy work on the genus Enterobacter. This group showed a very high genetic homogeneity since the average relative binding ratio of nine analysed strains is equal to 91%. The taxonomic position of this group into the family of enterobacteria is discussed with the species E. cloacae (37 to 61%), K. pneumoniae (44 to 60%), K. oxytoca (57-58%), L. malonatica (syn. Citrobacter intermedius, a:46 to 54%), L. amalonatica (syn. C. intermedius, b: 51%), and the group H3 (52-61%). The group H1 is defined on phenotypic and genetic data."}
{"id": "PMID:476549", "title": "Thiosulfate formation and associated isotope effects during sulfite reduction by Clostridium pasteurianum.", "content": "During growth of Clostridium pasteurianum on sulfite, approximately half the sulfite was reduced to sulfide and half to thiosulfate. Sulfide was enriched in 32S or 34S at different stages of growth and thiosulfate was enriched in 32S, particularly in the sulfane atom. It is suggested that thiosulfate in these bacterial cultures arose from a secondary chemical reaction. The chemical formation of thiosulfate from sulfide and sulfite was also accompanied by sulfur isotope fractionation. The implications of these results with respect to 'inverse' isotopic effects are discussed.", "contents": "Thiosulfate formation and associated isotope effects during sulfite reduction by Clostridium pasteurianum. During growth of Clostridium pasteurianum on sulfite, approximately half the sulfite was reduced to sulfide and half to thiosulfate. Sulfide was enriched in 32S or 34S at different stages of growth and thiosulfate was enriched in 32S, particularly in the sulfane atom. It is suggested that thiosulfate in these bacterial cultures arose from a secondary chemical reaction. The chemical formation of thiosulfate from sulfide and sulfite was also accompanied by sulfur isotope fractionation. The implications of these results with respect to 'inverse' isotopic effects are discussed."}
{"id": "PMID:476550", "title": "Minute tubular forms in soil.", "content": "Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be composed of parallel fibers held together by protein. Most of the tubules fell within a ranged of 10 to 50 nm in width. Lengths (of broken pieces) ranged from 0.2 to 1 micron or more. A few unbroken ends were found. They were rounded. Tubular structures of similar dimensions were found surrounding lysed cells in sectioned preparations of bacteria that had been separated from soil. The tubules were present in surface soils but not a subsurface sample. Their numbers decreased during bacterial multiplication in soil or broth containing soil. No evidence was found of cleared areas or increase in number of tubules when the tubules were plated with a heterogeneous microbial flora from soil.", "contents": "Minute tubular forms in soil. Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be composed of parallel fibers held together by protein. Most of the tubules fell within a ranged of 10 to 50 nm in width. Lengths (of broken pieces) ranged from 0.2 to 1 micron or more. A few unbroken ends were found. They were rounded. Tubular structures of similar dimensions were found surrounding lysed cells in sectioned preparations of bacteria that had been separated from soil. The tubules were present in surface soils but not a subsurface sample. Their numbers decreased during bacterial multiplication in soil or broth containing soil. No evidence was found of cleared areas or increase in number of tubules when the tubules were plated with a heterogeneous microbial flora from soil."}
{"id": "PMID:476551", "title": "Effect of oxygen on batch and continuous cultures of a nitrogen-fixing Arthrobacter sp.", "content": "Growth, acetylene reduction, and respiration rate were studied in batch and continuous cultures of Arthrobacter fluorescents at different oxygen partial pressures. The optimum pO2 values for growth and acetylene reduction were 0.05 and 0.025 atm, respectively, but microorganisms can tolerate higher pO2 values. The growth of cultures provided with combined nitrogen was dependent on oxygen availability, and strict anaerobic conditions did not support growth. Acetylene reduction of a population grown in continuous culture and adapted to low pO2 (0.02 atm) was much more sensitive to oxygenation than that of a population adapted to high pO2 (0.4 atm). Their maximum nitrogenase activity, at their optimal pO2 values, were quite different. The respiratory activity of nitrogen-fixing cultures increased with increasing oxygen tensions until a pO2 of 0.2 atm. At higher pO2 values, the respiration rate began to decrease.", "contents": "Effect of oxygen on batch and continuous cultures of a nitrogen-fixing Arthrobacter sp. Growth, acetylene reduction, and respiration rate were studied in batch and continuous cultures of Arthrobacter fluorescents at different oxygen partial pressures. The optimum pO2 values for growth and acetylene reduction were 0.05 and 0.025 atm, respectively, but microorganisms can tolerate higher pO2 values. The growth of cultures provided with combined nitrogen was dependent on oxygen availability, and strict anaerobic conditions did not support growth. Acetylene reduction of a population grown in continuous culture and adapted to low pO2 (0.02 atm) was much more sensitive to oxygenation than that of a population adapted to high pO2 (0.4 atm). Their maximum nitrogenase activity, at their optimal pO2 values, were quite different. The respiratory activity of nitrogen-fixing cultures increased with increasing oxygen tensions until a pO2 of 0.2 atm. At higher pO2 values, the respiration rate began to decrease."}
{"id": "PMID:476552", "title": "Investigation of antibodies against Fasciola hepatica in moose Alces americana.", "content": "A serological investigation was carried out to check the presence of antibodies against Fasciola hepatica in a collection of 125 sera moose Alces americana killed in the Province of Quebec. As revealed by hemagglutination tests, two moose had titers suggesting contact with this parasite.", "contents": "Investigation of antibodies against Fasciola hepatica in moose Alces americana. A serological investigation was carried out to check the presence of antibodies against Fasciola hepatica in a collection of 125 sera moose Alces americana killed in the Province of Quebec. As revealed by hemagglutination tests, two moose had titers suggesting contact with this parasite."}
{"id": "PMID:476553", "title": "Chlortetracycline and sulfonamide resistance of fecal bacteria in swine receiving medicated feed.", "content": "The fecal bacterial flora of swine receiving a ration supplemented with chlortetracycline, sulfamethazine, and penicillin was tested for resistance to chlortetracycline and sulfamethazine using anaerobic techniques and medium M-10. Approximately 15.5 and 1.4% of the flora grew in the presence of 25 and 100 microgram of tetracycline/mL, respectively. Higher numbers of bacteria grew in the presence of similar concentrations of sulfamethazine. Thirty-five chlortetracycline-resistant isolates were tentatively identified by genera. Nine different genera were identified, four of these were Gram-positive and five were Gram-negative. The most common genera isolated were Streptococcus and Eubacterium. This demonstrates that in the fecal flora of swine fed rations supplemented with chlortetra-cycline, a wide variety of bacterial genera can be resistant to this antibiotic.", "contents": "Chlortetracycline and sulfonamide resistance of fecal bacteria in swine receiving medicated feed. The fecal bacterial flora of swine receiving a ration supplemented with chlortetracycline, sulfamethazine, and penicillin was tested for resistance to chlortetracycline and sulfamethazine using anaerobic techniques and medium M-10. Approximately 15.5 and 1.4% of the flora grew in the presence of 25 and 100 microgram of tetracycline/mL, respectively. Higher numbers of bacteria grew in the presence of similar concentrations of sulfamethazine. Thirty-five chlortetracycline-resistant isolates were tentatively identified by genera. Nine different genera were identified, four of these were Gram-positive and five were Gram-negative. The most common genera isolated were Streptococcus and Eubacterium. This demonstrates that in the fecal flora of swine fed rations supplemented with chlortetra-cycline, a wide variety of bacterial genera can be resistant to this antibiotic."}
{"id": "PMID:476554", "title": "Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants.", "content": "Total cellular DNA from Rhizobium trifolii, R. melitoti, and R. japonicum strains 110 and 117 were prepared. DNA fragments generated with restriction endonuclease EcoRI from these DNA samples were compared in agarose gels after electrophoresis. DNA cleavage patterns generated from R. japonicum strain 110, R. trifolii, and R. meliloti were clearly distinguishable from each other. Restriction endonuclease cleavage patterns of DNA from R. japonicum strain 110 and presumptive R. trifolii mutant strains that nodulate soybean were found to be similar. Rhizobium trifolii mutant strains were also lysed by a phage specific for R. japonicum strain 110. These results show that \"R. trifolii mutant strains\" are indeed derivatives of R. japonicum strain 110 and not R. trifolii.", "contents": "Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants. Total cellular DNA from Rhizobium trifolii, R. melitoti, and R. japonicum strains 110 and 117 were prepared. DNA fragments generated with restriction endonuclease EcoRI from these DNA samples were compared in agarose gels after electrophoresis. DNA cleavage patterns generated from R. japonicum strain 110, R. trifolii, and R. meliloti were clearly distinguishable from each other. Restriction endonuclease cleavage patterns of DNA from R. japonicum strain 110 and presumptive R. trifolii mutant strains that nodulate soybean were found to be similar. Rhizobium trifolii mutant strains were also lysed by a phage specific for R. japonicum strain 110. These results show that \"R. trifolii mutant strains\" are indeed derivatives of R. japonicum strain 110 and not R. trifolii."}
{"id": "PMID:476555", "title": "The fine structure of mature and germinating chlamydospores of Fusarium oxysporum.", "content": "A number of features not described previously has been revealed in electron-microscope studies of mature chlamydospores of Fusarium oxysporum. On the maturation of one isolate, many spores formed a thick matrix-like layer containing electron-dense particles on the exterior surface of the spore wall. In thin sections of mature chlamydospores of the same isolate, cisternae of endoplasmic reticulum surrounding, and in close apposition to, the limiting boundary of the lipid bodies were revealed. The germination of chlamydospores was accompanied by (a) the rapid appearance of polysaccharide deposits and changes in the configuration of some subcellular organelles, (b) the formation of a new wall layer between the plasma membrane and the innermost layer of the spore wall, (c) the rupture of the outermost coats of the spore wall, and (d) the emergence of the germ tube as an extension of the new wall layer.", "contents": "The fine structure of mature and germinating chlamydospores of Fusarium oxysporum. A number of features not described previously has been revealed in electron-microscope studies of mature chlamydospores of Fusarium oxysporum. On the maturation of one isolate, many spores formed a thick matrix-like layer containing electron-dense particles on the exterior surface of the spore wall. In thin sections of mature chlamydospores of the same isolate, cisternae of endoplasmic reticulum surrounding, and in close apposition to, the limiting boundary of the lipid bodies were revealed. The germination of chlamydospores was accompanied by (a) the rapid appearance of polysaccharide deposits and changes in the configuration of some subcellular organelles, (b) the formation of a new wall layer between the plasma membrane and the innermost layer of the spore wall, (c) the rupture of the outermost coats of the spore wall, and (d) the emergence of the germ tube as an extension of the new wall layer."}
{"id": "PMID:476556", "title": "Bacterial utilization of a hydrazine derivative as nitrogen source for growth.", "content": "The ability of microbes to metabolize the N--N bond seems rare. Pseudomonas sp. from soil can utilize 1,4,5,6-tetrahydro-6-oxo-3-pyridazinecarboxylic acid as C and N source. This appears to be the first report that a microbe can cleave a nitrogen--nitrogen bond in an organic compound and use the products for growth.", "contents": "Bacterial utilization of a hydrazine derivative as nitrogen source for growth. The ability of microbes to metabolize the N--N bond seems rare. Pseudomonas sp. from soil can utilize 1,4,5,6-tetrahydro-6-oxo-3-pyridazinecarboxylic acid as C and N source. This appears to be the first report that a microbe can cleave a nitrogen--nitrogen bond in an organic compound and use the products for growth."}
{"id": "PMID:476557", "title": "The ultrastructure of the major species of an enriched methanogenic culture utilizing acetic acid.", "content": "The ultrastructure of the cells of the major component of an enriched culture of a presumed methanogen which utilized acetic acid was studied by transmission and scanning electron microscopy. The filaments were composed of Gram-positive, rod-shaped cells, 1--2 micrometer in length and about 0.5 micrometer in breadth, attached end to end. Septa between cells were complex, with a central, electron-dense sheet which had a spherical enlargement in the center separating the cell walls. The cells walls themselves were of variable thickness with a light, fluffy, thin portion on the outside and a denser, thicker portion within. They contain a series of rings stacked side by side which are composed of material that stains strongly and positively with phosphotungstate ion. The cytoplasmic membrane of these cells had an outer leaflet which stains more densely with uranium and lead ions than the inner leaflet. There were no recognizable organelles in the cytoplasm other than ribosomes. It is shown in these observations that the presumed methanogen may likely be a new species.", "contents": "The ultrastructure of the major species of an enriched methanogenic culture utilizing acetic acid. The ultrastructure of the cells of the major component of an enriched culture of a presumed methanogen which utilized acetic acid was studied by transmission and scanning electron microscopy. The filaments were composed of Gram-positive, rod-shaped cells, 1--2 micrometer in length and about 0.5 micrometer in breadth, attached end to end. Septa between cells were complex, with a central, electron-dense sheet which had a spherical enlargement in the center separating the cell walls. The cells walls themselves were of variable thickness with a light, fluffy, thin portion on the outside and a denser, thicker portion within. They contain a series of rings stacked side by side which are composed of material that stains strongly and positively with phosphotungstate ion. The cytoplasmic membrane of these cells had an outer leaflet which stains more densely with uranium and lead ions than the inner leaflet. There were no recognizable organelles in the cytoplasm other than ribosomes. It is shown in these observations that the presumed methanogen may likely be a new species."}
{"id": "PMID:476558", "title": "[Study of the structural polypeptides of Chilo suppressalis iridescent virus (Iridovirus type 6)].", "content": "We report a procedure for the purification of Chilo iridescent virus (Iridovirus type 6), an evaluation of the purification procedure, and the results of analyses of the virion proteins by acrylamide gel electrophoresis. Purity was evaluated in three ways, i.e., by analysis of purified virions from artificial mixtures of infected and labeled uninfected larvae, electrophoresis at neutral pH, and electron-microscopic examination. Analysis of the polypeptides of purified CIV gave the following results: (i) after solubilization with SDS-B-mercaptoethanol, 16 polypeptides could be resolved in Coomassie brillant blue-stained electrophoretograms with molecular weights ranging from 18,000 to 115,000; (ii) after solubilization with SDS-urea, 26 polypeptides could be resolved with molecular weights ranging from 10,000 to 230,000 daltons.", "contents": "[Study of the structural polypeptides of Chilo suppressalis iridescent virus (Iridovirus type 6)]. We report a procedure for the purification of Chilo iridescent virus (Iridovirus type 6), an evaluation of the purification procedure, and the results of analyses of the virion proteins by acrylamide gel electrophoresis. Purity was evaluated in three ways, i.e., by analysis of purified virions from artificial mixtures of infected and labeled uninfected larvae, electrophoresis at neutral pH, and electron-microscopic examination. Analysis of the polypeptides of purified CIV gave the following results: (i) after solubilization with SDS-B-mercaptoethanol, 16 polypeptides could be resolved in Coomassie brillant blue-stained electrophoretograms with molecular weights ranging from 18,000 to 115,000; (ii) after solubilization with SDS-urea, 26 polypeptides could be resolved with molecular weights ranging from 10,000 to 230,000 daltons."}
{"id": "PMID:476559", "title": "Evidence of a competitive hierarchy among coprophilous fungal populations.", "content": "Evidence is presented that interference competition may be important in later states of fungal colonization of cattle feces from a semiarid grassland in Colorado. Cultural antagonism was examined among fungal isolates representing early sporulating colonists (Ascobolus furfuraceus and Saccobolus truncatus), later sporulating colonists (Iodophanus carneus, Coniochaeta discospora, Hypocopra merdaria, and Poronia punctata), and one early successional species that is able to persist (sporulate) through later stages (Podospora decipiens). Poronia punctata, a comparatively slower-growing and later-appearing colonist (18- to 54-month-old fecal pats), is uniformly antagonistic to all of seven earlier-appearing and co-occurring fungal species. Antibiosis is believed to account for the observed antagonism. The authors suggest that the evolutionary product of interference competition among coprophilous fungal populations may be a pattern of competitive hierarchy in which certain slower-growing, later-successional species can limit the reproductive potential of other fungal colonists on fecal substrates.", "contents": "Evidence of a competitive hierarchy among coprophilous fungal populations. Evidence is presented that interference competition may be important in later states of fungal colonization of cattle feces from a semiarid grassland in Colorado. Cultural antagonism was examined among fungal isolates representing early sporulating colonists (Ascobolus furfuraceus and Saccobolus truncatus), later sporulating colonists (Iodophanus carneus, Coniochaeta discospora, Hypocopra merdaria, and Poronia punctata), and one early successional species that is able to persist (sporulate) through later stages (Podospora decipiens). Poronia punctata, a comparatively slower-growing and later-appearing colonist (18- to 54-month-old fecal pats), is uniformly antagonistic to all of seven earlier-appearing and co-occurring fungal species. Antibiosis is believed to account for the observed antagonism. The authors suggest that the evolutionary product of interference competition among coprophilous fungal populations may be a pattern of competitive hierarchy in which certain slower-growing, later-successional species can limit the reproductive potential of other fungal colonists on fecal substrates."}
{"id": "PMID:476560", "title": "The effect of 5-fluorouracil and adriamycin on heterotransplantation of Noble rat prostatic tumors in congenitally athymic (nude) mice.", "content": "The Nb rat prostatic adenocarcinoma is a well-characterized, hormonally induced family of tumors that are all readily transplantable into congenitally athymic (nude) mice. Because of this versatile heterotransplantation model, multiple replicate copies of individual tumors can be studied \"in rodent.\" We have extended this by studying the chemotherapeutic response of such tumors and believe that this provides a useful avenue for evaluation of cytotoxic agents. Indeed, this combination of both animal model systems may provide a useful experimental tool to evaluate tumor growth, histopathologic changes and responsiveness to appropriate therapy. We report herein that two Nb rat prostatic carcinomas (2 Pr-129-D-11A and Pr-90) and thie responsiveness to Adriamycin and 5-fluorouracil are objective by studying both growth rates and tumor histology.", "contents": "The effect of 5-fluorouracil and adriamycin on heterotransplantation of Noble rat prostatic tumors in congenitally athymic (nude) mice. The Nb rat prostatic adenocarcinoma is a well-characterized, hormonally induced family of tumors that are all readily transplantable into congenitally athymic (nude) mice. Because of this versatile heterotransplantation model, multiple replicate copies of individual tumors can be studied \"in rodent.\" We have extended this by studying the chemotherapeutic response of such tumors and believe that this provides a useful avenue for evaluation of cytotoxic agents. Indeed, this combination of both animal model systems may provide a useful experimental tool to evaluate tumor growth, histopathologic changes and responsiveness to appropriate therapy. We report herein that two Nb rat prostatic carcinomas (2 Pr-129-D-11A and Pr-90) and thie responsiveness to Adriamycin and 5-fluorouracil are objective by studying both growth rates and tumor histology."}
{"id": "PMID:476562", "title": "Remission of giant lymph node hyperplasia with anemia after radiotherapy.", "content": "We have described a 51-year-old patient with unresectable mesenteric giant lymph node hyperplasia of the plasma cell type, severe systemic manifestations, and profound anemia. Supression of erythropoiesis may have been related to the presence of a circulating erythropoietic inhibitor produced by the lymphoid tumor. Markedly elevated titers to Epstein-Barr virus capsid antigen suggest that this virus may be important in the etiology of the abnormal lymphoid proliferation. The marked clinical response and decrease in the size of the tumor following irradiation suggests that radiation therapy may be an alternative form of treatment for similar patients with unresectable lesions.", "contents": "Remission of giant lymph node hyperplasia with anemia after radiotherapy. We have described a 51-year-old patient with unresectable mesenteric giant lymph node hyperplasia of the plasma cell type, severe systemic manifestations, and profound anemia. Supression of erythropoiesis may have been related to the presence of a circulating erythropoietic inhibitor produced by the lymphoid tumor. Markedly elevated titers to Epstein-Barr virus capsid antigen suggest that this virus may be important in the etiology of the abnormal lymphoid proliferation. The marked clinical response and decrease in the size of the tumor following irradiation suggests that radiation therapy may be an alternative form of treatment for similar patients with unresectable lesions."}
{"id": "PMID:476564", "title": "Slipped capital femoral epiphysis (SCFE) following pelvic irradiation for rhabdomyosarcoma.", "content": "A 7-year-old child developed slipped capital femoral epiphysis (SCFE) 3 years following pelvic irradiation for rhabdomyosarcoma. The reasons for this occurrence are discussed. This is the second report of radiation-induced SCFE and it is anticipated that more such cases are likely to occur.", "contents": "Slipped capital femoral epiphysis (SCFE) following pelvic irradiation for rhabdomyosarcoma. A 7-year-old child developed slipped capital femoral epiphysis (SCFE) 3 years following pelvic irradiation for rhabdomyosarcoma. The reasons for this occurrence are discussed. This is the second report of radiation-induced SCFE and it is anticipated that more such cases are likely to occur."}
{"id": "PMID:476563", "title": "Gallium67 scanning compared with physical examination in the preoperative staging of malignant melanoma.", "content": "Sixty patients with Stage II malignant melanoma were evaluated in order to compare preoperative gallium67 total body scan, physical examination findings, and ultimate histologic findings in regard to the site of the primary lesion and status of the regional lymph nodes. Physical examination was a more efficient and a more sensitive method of evaluation compared to gallium67 scanning in these patients when assessing nodal involvement.", "contents": "Gallium67 scanning compared with physical examination in the preoperative staging of malignant melanoma. Sixty patients with Stage II malignant melanoma were evaluated in order to compare preoperative gallium67 total body scan, physical examination findings, and ultimate histologic findings in regard to the site of the primary lesion and status of the regional lymph nodes. Physical examination was a more efficient and a more sensitive method of evaluation compared to gallium67 scanning in these patients when assessing nodal involvement."}
{"id": "PMID:476565", "title": "Inhibition of spontaneous AKR leukemia by multiple inoculations of Corynebacterium parvum.", "content": "In previous studies we have shown that AKR mice could be protected from spontaneous leukemia by specific immunotherapy in combination with splenectomy. In this experiment we investigated the effects of nonspecific immunotherapy with C. parvum in a similar regimen. It was found that bi-weekly, ip inoculations of 0.7 mg of C. parvum could significantly protect AKR mice from spontaneous tumors, and that splenectomy could not modify this effect. Thus, the spleen does not appear to play a determinant role in mediating the protective effects of C. parvum, as had been suggested in other systems.", "contents": "Inhibition of spontaneous AKR leukemia by multiple inoculations of Corynebacterium parvum. In previous studies we have shown that AKR mice could be protected from spontaneous leukemia by specific immunotherapy in combination with splenectomy. In this experiment we investigated the effects of nonspecific immunotherapy with C. parvum in a similar regimen. It was found that bi-weekly, ip inoculations of 0.7 mg of C. parvum could significantly protect AKR mice from spontaneous tumors, and that splenectomy could not modify this effect. Thus, the spleen does not appear to play a determinant role in mediating the protective effects of C. parvum, as had been suggested in other systems."}
{"id": "PMID:476566", "title": "Plasma IgA, IgG and IgM and their relationship to breast cancer in British, Japanese and Hawaiian-Japanese women.", "content": "The plasma levels of immunoglobulins IgA, IgG and IgM have been measured in 35 British, 44 Hawaiian-Japanese and 37 Japanese healthy adult women. Previous investigations showed that the mean levels of all three immunoglobulins were higher in Japanese than in British normal women. The present study finds that Hawaiian-Japanese women have \"Japanese\" levels of IgA, \"British\" levels of IgM and are intermediate for IgG. Thus, plasma IgM concentrations correlate with breast cancer incidence rates in the three racial groups and the reduced amounts of plasma IgM found in Japanese patients with breast cancer support this association.", "contents": "Plasma IgA, IgG and IgM and their relationship to breast cancer in British, Japanese and Hawaiian-Japanese women. The plasma levels of immunoglobulins IgA, IgG and IgM have been measured in 35 British, 44 Hawaiian-Japanese and 37 Japanese healthy adult women. Previous investigations showed that the mean levels of all three immunoglobulins were higher in Japanese than in British normal women. The present study finds that Hawaiian-Japanese women have \"Japanese\" levels of IgA, \"British\" levels of IgM and are intermediate for IgG. Thus, plasma IgM concentrations correlate with breast cancer incidence rates in the three racial groups and the reduced amounts of plasma IgM found in Japanese patients with breast cancer support this association."}
{"id": "PMID:476567", "title": "Intralesional immunotherapy of malignant melanoma with mycobacterium smegmatis cell wall skeleton combined with trehalose dimycolate (P3).", "content": "The clinical efficacy of intralesional immunotherapy utilizing Mycobacterium smegmatis cell wall skeleton (CWS) and trehalose dimycolate attached to oil droplets was investigated in 15 patients with advanced malignant melanoma. Patients received 300 microgram to 1050 microgram of the CWS combined with one-half that amount of trehalose dimycolate every 1 to 2 weeks for a total of 8 treatments. Therapy was continued if regression of injected lesions only occurred. Therapy was discontinued if regression of noninjected disease also occurred. Six of the 15 patients had regression of at least one injected lesion. Four of these 6 patients also had regression of noninjected disease lasting 4+, 6, 16 and 18+ months. Response was highly related to immune status. Six (83%) of 7 patients who reacted to one of a battery of skin tests responded. All 8 patients who did not react to skin tests failed to respond to therapy. There was no correlation of response with sex, prior therapy, disease-free interval or presence of visceral disease. Mycobacterial CWS and trehalose dimycolate is an effective immunotherapeutic agent. Additional studies of purified immunoadjuvants are warranted.", "contents": "Intralesional immunotherapy of malignant melanoma with mycobacterium smegmatis cell wall skeleton combined with trehalose dimycolate (P3). The clinical efficacy of intralesional immunotherapy utilizing Mycobacterium smegmatis cell wall skeleton (CWS) and trehalose dimycolate attached to oil droplets was investigated in 15 patients with advanced malignant melanoma. Patients received 300 microgram to 1050 microgram of the CWS combined with one-half that amount of trehalose dimycolate every 1 to 2 weeks for a total of 8 treatments. Therapy was continued if regression of injected lesions only occurred. Therapy was discontinued if regression of noninjected disease also occurred. Six of the 15 patients had regression of at least one injected lesion. Four of these 6 patients also had regression of noninjected disease lasting 4+, 6, 16 and 18+ months. Response was highly related to immune status. Six (83%) of 7 patients who reacted to one of a battery of skin tests responded. All 8 patients who did not react to skin tests failed to respond to therapy. There was no correlation of response with sex, prior therapy, disease-free interval or presence of visceral disease. Mycobacterial CWS and trehalose dimycolate is an effective immunotherapeutic agent. Additional studies of purified immunoadjuvants are warranted."}
{"id": "PMID:476568", "title": "Electron microscopy of Hodgkin's disease tissue cultures.", "content": "Cells from 9 monolayer tissue cultures prepared from Hodgkin's disease tumors in the spleen were examined in the electron microscope. Three established culture lines (carried in vitro for greater than 3 years and passaged greater than 200 times) that contained aneuploid karyotypes were composed of oval cells with numerous interdigitating surface microvilli. The nuclei were complex and convoluted with multiple large nucleoli and dispersed chromatin. The cytoplasm contained lysosomes, microfilaments, a complex Golgi apparatus, nondilated rough endoplasmic reticulum, polyribosomes, fat, and glycogen. One Hodgkin's disease monolayer with aneuploid chromosomes examined from the 4th to 48th passage in culture was composed of larger cells with fewer microvilli and numerous multinuclear giant cells. Two monolayers derived from transplanted tumors in nude mice inoculated with Hodgkin's disease cultured cells were similar to the original cell lines. The ultrastructural features of these 6 cultures with aneuploid karyotypes differed from those of 3 monolayers which, although prepared from Hodgkin's disease splenic tumors, were composed of fibroblastic cells with diploid chromosomes. The aneuploid Hodgkin's disease cultures did not resemble 6 normal spleen, thymus, or lung monolayers, Raji lymphoblastoid suspension cultures, or Hela cells. Our electron microscopic studies indicate that adherent cells which replicate in some monolayer tissue cultures derived from Hodgkin's disease tumors are related to and possibly derived from neoplastic macrophages.", "contents": "Electron microscopy of Hodgkin's disease tissue cultures. Cells from 9 monolayer tissue cultures prepared from Hodgkin's disease tumors in the spleen were examined in the electron microscope. Three established culture lines (carried in vitro for greater than 3 years and passaged greater than 200 times) that contained aneuploid karyotypes were composed of oval cells with numerous interdigitating surface microvilli. The nuclei were complex and convoluted with multiple large nucleoli and dispersed chromatin. The cytoplasm contained lysosomes, microfilaments, a complex Golgi apparatus, nondilated rough endoplasmic reticulum, polyribosomes, fat, and glycogen. One Hodgkin's disease monolayer with aneuploid chromosomes examined from the 4th to 48th passage in culture was composed of larger cells with fewer microvilli and numerous multinuclear giant cells. Two monolayers derived from transplanted tumors in nude mice inoculated with Hodgkin's disease cultured cells were similar to the original cell lines. The ultrastructural features of these 6 cultures with aneuploid karyotypes differed from those of 3 monolayers which, although prepared from Hodgkin's disease splenic tumors, were composed of fibroblastic cells with diploid chromosomes. The aneuploid Hodgkin's disease cultures did not resemble 6 normal spleen, thymus, or lung monolayers, Raji lymphoblastoid suspension cultures, or Hela cells. Our electron microscopic studies indicate that adherent cells which replicate in some monolayer tissue cultures derived from Hodgkin's disease tumors are related to and possibly derived from neoplastic macrophages."}
{"id": "PMID:476569", "title": "Necrolytic migratory erythema, presenting as candidiasis, due to a pancreatic glucagonoma.", "content": "A 54-year-old male with diabetes, weight loss, glossitis and Candidiasis presented with the typical cutaneous eruption of necrolytic migratory erythema. The suspicion of pancreatic glucagonoma was confirmed by an elevated plasma glucagon level. Surgical removal of the pancreatic alpha cell tumor resulted in a complete disappearance of all symptoms. The importance of the recognition of the skin eruption of necrolytic migratory erythema as a clue to the presence of pancreatic glucagonoma is emphasized.", "contents": "Necrolytic migratory erythema, presenting as candidiasis, due to a pancreatic glucagonoma. A 54-year-old male with diabetes, weight loss, glossitis and Candidiasis presented with the typical cutaneous eruption of necrolytic migratory erythema. The suspicion of pancreatic glucagonoma was confirmed by an elevated plasma glucagon level. Surgical removal of the pancreatic alpha cell tumor resulted in a complete disappearance of all symptoms. The importance of the recognition of the skin eruption of necrolytic migratory erythema as a clue to the presence of pancreatic glucagonoma is emphasized."}
{"id": "PMID:476571", "title": "Significance of squamous components in endometrioid carcinoma of the ovary.", "content": "Forty-nine cases of ovarian endometrioid carcinoma with squamous foci were reviewed. Of particular interest was the biologic behavior of the neoplasms in relation to the appearance of the epithelium. As previously noted for the analogous uterine endometrial tumors, the ovarian adenosquamous lesions occurred later in life, were more advanced, were associated with less differentiated adenocarcinomatous components, and had a poorer prognosis. The five-year survival rate for patients with adenosquamous endometrioid ovarian carcinoma was 21% in comparison with the 90% survival found in patients with ovarian endometrioid adenocanthoma.", "contents": "Significance of squamous components in endometrioid carcinoma of the ovary. Forty-nine cases of ovarian endometrioid carcinoma with squamous foci were reviewed. Of particular interest was the biologic behavior of the neoplasms in relation to the appearance of the epithelium. As previously noted for the analogous uterine endometrial tumors, the ovarian adenosquamous lesions occurred later in life, were more advanced, were associated with less differentiated adenocarcinomatous components, and had a poorer prognosis. The five-year survival rate for patients with adenosquamous endometrioid ovarian carcinoma was 21% in comparison with the 90% survival found in patients with ovarian endometrioid adenocanthoma."}
{"id": "PMID:476570", "title": "Ultrastructure of a primary fibrosarcoma of the human thyroid gland.", "content": "A primary fibrosarcoma of the thyroid occurring in a patient with a nodular goiter is described. Light microscopy showed interwoven bundles of spindle cells admixed with plump ovoid cells, and foci of multinucleated giant cells. Remnants of distorted thyroid follicles were found only at the periphery of the tumor. \"Transitional\" epithelial elements were not discernible but the tumor resembled an anaplastic giant cell carcinoma. Multiple blocks studied by electron microscopy, however, revealed that the tumor cells, including the giant cells, have the ultrastructure features of a fibroblast.", "contents": "Ultrastructure of a primary fibrosarcoma of the human thyroid gland. A primary fibrosarcoma of the thyroid occurring in a patient with a nodular goiter is described. Light microscopy showed interwoven bundles of spindle cells admixed with plump ovoid cells, and foci of multinucleated giant cells. Remnants of distorted thyroid follicles were found only at the periphery of the tumor. \"Transitional\" epithelial elements were not discernible but the tumor resembled an anaplastic giant cell carcinoma. Multiple blocks studied by electron microscopy, however, revealed that the tumor cells, including the giant cells, have the ultrastructure features of a fibroblast."}
{"id": "PMID:476572", "title": "Clear-cell chondrosarcoma: a report of five cases including ultrastructural study.", "content": "Five cases of clear-cell variant of chondrosarcoma (Unni et al.) are reported. The tumors occurred in the epiphyseal region of long bones; three in the femoral head. Roentgenographically, the lesion was usually a well-defined and benign appearing one, either purely lytic (3 cases) or with central radiodensity (2 cases). Histologically, all five cases had areas of conventional chondrosarcoma; however, the greater portion of the tumor was made up of sheets of clear-cells intermixed with nonneoplastic bone trabeculae but devoid of chondroid matrix. Electron microscopic studies showed that these clear-cells possess cytoplasmic microvilli, abundant glycogen particles and prominent golgi complexes, like normal or tumorous chondroid cells usually have. In our experience, the best treatment seemed to be en bloc resection with joint replacement; indeed, despite the fact that they are true chondrosarcomas, these tumors usually have a very slow rate of growth.", "contents": "Clear-cell chondrosarcoma: a report of five cases including ultrastructural study. Five cases of clear-cell variant of chondrosarcoma (Unni et al.) are reported. The tumors occurred in the epiphyseal region of long bones; three in the femoral head. Roentgenographically, the lesion was usually a well-defined and benign appearing one, either purely lytic (3 cases) or with central radiodensity (2 cases). Histologically, all five cases had areas of conventional chondrosarcoma; however, the greater portion of the tumor was made up of sheets of clear-cells intermixed with nonneoplastic bone trabeculae but devoid of chondroid matrix. Electron microscopic studies showed that these clear-cells possess cytoplasmic microvilli, abundant glycogen particles and prominent golgi complexes, like normal or tumorous chondroid cells usually have. In our experience, the best treatment seemed to be en bloc resection with joint replacement; indeed, despite the fact that they are true chondrosarcomas, these tumors usually have a very slow rate of growth."}
{"id": "PMID:476573", "title": "Biochemical studies of the matrix of craniovertebral chordoma and a metastasis.", "content": "Glycosaminoglycans of human chordoma and a metastasis were studied. The glycosaminoglycans were derived from the extracellular matrix and consisted of chondroitin 4- and 6-sulphate, keratan sulphate and hyaluronate. The ratio of chondroitin sulphate to keratan sulphate was much lower in the metastasis than in the primary. Proteoglycan extracted with 4M guanidinium chloride and purified by associative density-gradient centrifugation was assessed on Sepharose 2B before and after reduction and alkylation. The result of this experiment suggested that only a small proportion of the proteoglycans were aggregated.", "contents": "Biochemical studies of the matrix of craniovertebral chordoma and a metastasis. Glycosaminoglycans of human chordoma and a metastasis were studied. The glycosaminoglycans were derived from the extracellular matrix and consisted of chondroitin 4- and 6-sulphate, keratan sulphate and hyaluronate. The ratio of chondroitin sulphate to keratan sulphate was much lower in the metastasis than in the primary. Proteoglycan extracted with 4M guanidinium chloride and purified by associative density-gradient centrifugation was assessed on Sepharose 2B before and after reduction and alkylation. The result of this experiment suggested that only a small proportion of the proteoglycans were aggregated."}
{"id": "PMID:476574", "title": "Family study of serum carcinoembryonic antigen in inherited medullary carcinoma of the thyroid.", "content": "Serum carcinoembryonic antigen (CEA) and calcitonin were assayed in 8 patients with medullary carcinoma of the thyroid (MCT) and 14 unaffected family members, from 4 pedigrees of Sipple's syndrome and one pedigree with inherited MCT. Serum CEA ranged from 5.4 to 44.5 ng/ml in the patients, but less than 2.5 ng/ml in the unaffected. This is in contrast with retinoblastoma and cancer family syndrome, where not only the patients but also unaffected family members show high serum CEA, and cytoplasmic or selective maternal inheritance of serum CEA level is presumed. In inheritable MCT, serum CEA increases in association with the development of MCT, and serum CEA level per se is not inherited.", "contents": "Family study of serum carcinoembryonic antigen in inherited medullary carcinoma of the thyroid. Serum carcinoembryonic antigen (CEA) and calcitonin were assayed in 8 patients with medullary carcinoma of the thyroid (MCT) and 14 unaffected family members, from 4 pedigrees of Sipple's syndrome and one pedigree with inherited MCT. Serum CEA ranged from 5.4 to 44.5 ng/ml in the patients, but less than 2.5 ng/ml in the unaffected. This is in contrast with retinoblastoma and cancer family syndrome, where not only the patients but also unaffected family members show high serum CEA, and cytoplasmic or selective maternal inheritance of serum CEA level is presumed. In inheritable MCT, serum CEA increases in association with the development of MCT, and serum CEA level per se is not inherited."}
{"id": "PMID:476575", "title": "Relationship between proliferative activity and estrogen receptors in breast cancer.", "content": "The proliferative activity of breast cancer has been analyzed in relation to the hormonal characteristics of the host and of the tumor for 199 patients. The analyses of labeling index frequency distributions of estrogen receptor positive (ER+) and negative (ER-) cancers from premenopausal and postmenopausal patients have allowed us to identify three different kinetic groups. A first group, with a very low proliferative activity, includes ER+ cancers from postmenopausal patients; a second group, with an intermediate proliferative activity, includes ER+ cancers from premenopausal and ER- cancers from postmenopausal patients; and a third group, with a very high proliferative activity, includes ER- cancers from premenopausal patients. Generally, the amount of estrogen receptors in ER+ cancers is inversely correlated with the proliferative activity. Lower levels of ER in premenopausal in comparison to postmenopausal patients were found in low proliferative activity tumors.", "contents": "Relationship between proliferative activity and estrogen receptors in breast cancer. The proliferative activity of breast cancer has been analyzed in relation to the hormonal characteristics of the host and of the tumor for 199 patients. The analyses of labeling index frequency distributions of estrogen receptor positive (ER+) and negative (ER-) cancers from premenopausal and postmenopausal patients have allowed us to identify three different kinetic groups. A first group, with a very low proliferative activity, includes ER+ cancers from postmenopausal patients; a second group, with an intermediate proliferative activity, includes ER+ cancers from premenopausal and ER- cancers from postmenopausal patients; and a third group, with a very high proliferative activity, includes ER- cancers from premenopausal patients. Generally, the amount of estrogen receptors in ER+ cancers is inversely correlated with the proliferative activity. Lower levels of ER in premenopausal in comparison to postmenopausal patients were found in low proliferative activity tumors."}
{"id": "PMID:476576", "title": "Prognostic value of estrogen receptors in primary breast cancer.", "content": "The estrogen receptor status in 335 primary breast carcinomas was correlated with disease-free interval, survival and site of recurrent disease. Estrogen receptor positive carcinomas had a longer disease-free interval, a longer survival (mastectomy-death) and a longer time interval between recurrence and death. These parameters were also influenced by the lymph node status at mastectomy. Estrogen receptor positive cancers had a significantly better chance of survival independent of lymph node status. Estrogen receptors also delayed recurrence in node-positive carcinomas, but this advantage gradually disappeared with increasing interval after mastectomy. Estrogen receptor positive or estrogen receptor negative primary carcinomas did not show any predilection for spread to any particular site.", "contents": "Prognostic value of estrogen receptors in primary breast cancer. The estrogen receptor status in 335 primary breast carcinomas was correlated with disease-free interval, survival and site of recurrent disease. Estrogen receptor positive carcinomas had a longer disease-free interval, a longer survival (mastectomy-death) and a longer time interval between recurrence and death. These parameters were also influenced by the lymph node status at mastectomy. Estrogen receptor positive cancers had a significantly better chance of survival independent of lymph node status. Estrogen receptors also delayed recurrence in node-positive carcinomas, but this advantage gradually disappeared with increasing interval after mastectomy. Estrogen receptor positive or estrogen receptor negative primary carcinomas did not show any predilection for spread to any particular site."}
{"id": "PMID:476577", "title": "Calcitonin as a marker for bronchogenic cancer: a prospective study.", "content": "A prospective study was done of serum calcitonin (HCT) levels in 61 patients with bronchogenic cancer. Initially, 52% of patients had hypercalcitonemia. Hypercalcitonemia was not confined to patients with any particular histologic type. Seventy-eight percent of those with high calcitonin remained normocalcemic. There was no correlation between high calcitonin levels and osseous metastases. Selective thyroid venous sampling delineated two types of hypercalcitonemia: thyroidal and ectopic. To date, the ectopic type has been associated with the small cell bronchogenic carcinoma. High initial calcitonin levels decreased significantly in 75% of patients on antitumor therapy. In 13 evaluable patients calcitonin levels mirrored clinical status changes 67% of the time. Calcitonin may be a useful marker to assess the results of therapy in patients with bronchogenic cancer.", "contents": "Calcitonin as a marker for bronchogenic cancer: a prospective study. A prospective study was done of serum calcitonin (HCT) levels in 61 patients with bronchogenic cancer. Initially, 52% of patients had hypercalcitonemia. Hypercalcitonemia was not confined to patients with any particular histologic type. Seventy-eight percent of those with high calcitonin remained normocalcemic. There was no correlation between high calcitonin levels and osseous metastases. Selective thyroid venous sampling delineated two types of hypercalcitonemia: thyroidal and ectopic. To date, the ectopic type has been associated with the small cell bronchogenic carcinoma. High initial calcitonin levels decreased significantly in 75% of patients on antitumor therapy. In 13 evaluable patients calcitonin levels mirrored clinical status changes 67% of the time. Calcitonin may be a useful marker to assess the results of therapy in patients with bronchogenic cancer."}
{"id": "PMID:476578", "title": "Multicystic, peritoneal mesothelioma: a report with electron microscopy of a case mimicking intra-abdominal cystic hygroma (lymphangioma).", "content": "A 27-year-old Caucasian female complained of persistent, poorly localized abdominal pain of several months' duration, found to be due to a multicystic diffuse lesion involving omentum, peritoneum and pelvic viscera. Three resections were required within a ten-month period for symptomatic control. The tumor showed many of the clinical, operative, gross and light microscopic features of intra-abdominal lymphagioma. Tissues removed at the third operation, however, were examined by electron microscopy, and found to consist of a myriad of small cysts and channels lined by mesothelial cells. In view of these findings, the lesion was regarded as a cystic peritoneal mesothelioma. Similarities between lymphangioma and peritoneal mesothelioma are discussed. It is suggested that electron microscopic examination of lining cells of cystic lesions which are considered grossly consistent with lymphagiomas may yield additional similar cases.", "contents": "Multicystic, peritoneal mesothelioma: a report with electron microscopy of a case mimicking intra-abdominal cystic hygroma (lymphangioma). A 27-year-old Caucasian female complained of persistent, poorly localized abdominal pain of several months' duration, found to be due to a multicystic diffuse lesion involving omentum, peritoneum and pelvic viscera. Three resections were required within a ten-month period for symptomatic control. The tumor showed many of the clinical, operative, gross and light microscopic features of intra-abdominal lymphagioma. Tissues removed at the third operation, however, were examined by electron microscopy, and found to consist of a myriad of small cysts and channels lined by mesothelial cells. In view of these findings, the lesion was regarded as a cystic peritoneal mesothelioma. Similarities between lymphangioma and peritoneal mesothelioma are discussed. It is suggested that electron microscopic examination of lining cells of cystic lesions which are considered grossly consistent with lymphagiomas may yield additional similar cases."}
{"id": "PMID:476580", "title": "Giant cell tumor in Paget's disease of bone: familial and geographic clustering.", "content": "Four patients with benign giant cell tumor and one patient with probable benign giant cell tumor associated with Paget's disease of bone are reported. The familial and geographic clustering of these cases is unique in that three patients were related and all patients traced ancestral roots to the same area of southern Italy. Tumors arose from the cranial or facial bones in three patients and from vertebral bodies in two patients. All caused symptoms by local compression, and treatment by curettage or radiotherapy was successful in all patients. Three separate tumors in one patient shrank dramatically in response to treatment with high doses of dexamethasone, and one patient whose tumor caused spinal cord compression showed marked improvement in neurologic function on therapy with dexamathasone.", "contents": "Giant cell tumor in Paget's disease of bone: familial and geographic clustering. Four patients with benign giant cell tumor and one patient with probable benign giant cell tumor associated with Paget's disease of bone are reported. The familial and geographic clustering of these cases is unique in that three patients were related and all patients traced ancestral roots to the same area of southern Italy. Tumors arose from the cranial or facial bones in three patients and from vertebral bodies in two patients. All caused symptoms by local compression, and treatment by curettage or radiotherapy was successful in all patients. Three separate tumors in one patient shrank dramatically in response to treatment with high doses of dexamethasone, and one patient whose tumor caused spinal cord compression showed marked improvement in neurologic function on therapy with dexamathasone."}
{"id": "PMID:476581", "title": "Male breast cancer: a natural history study.", "content": "Eighty-seven cases of male breast cancer seen over a 30-year period were reviewed. The overall observed and corrected five-year survival rates of 42% and 53% correspond well with the results in other series. Factors predicting disease-free interval and survival included size of primary lesion, ipsilateral axillary status, and presence of ulceration, which appear to be similar to those observed for female breast cancer. Eighteen patients (21%) had a second primary tumor (seven with cutaneous malignancies) and were of an older age group with a higher incidence of positive family history of breast cancer as well as other tumors. The overall prognosis of breast cancer in males does not appear to be less favorable than that in females.", "contents": "Male breast cancer: a natural history study. Eighty-seven cases of male breast cancer seen over a 30-year period were reviewed. The overall observed and corrected five-year survival rates of 42% and 53% correspond well with the results in other series. Factors predicting disease-free interval and survival included size of primary lesion, ipsilateral axillary status, and presence of ulceration, which appear to be similar to those observed for female breast cancer. Eighteen patients (21%) had a second primary tumor (seven with cutaneous malignancies) and were of an older age group with a higher incidence of positive family history of breast cancer as well as other tumors. The overall prognosis of breast cancer in males does not appear to be less favorable than that in females."}
{"id": "PMID:476582", "title": "Blindness as initial manifestation of meningeal carcinomatosis in breast cancer.", "content": "The sudden onset of blindness in adults, with or without a history of malignancy should raise the possibility of meningeal carcinomatosis. The diagnosis is best confirmed with examination of the spinal fluid. The actual mechanism by which blindness occurs is probably a combination of tumor cuffing around the optic nerve, direct infiltration and chronic papilledema. We are reporting two patients with disseminated breast cancer who presented with blindness as the first manifestation of meningeal involvement. Treatment with whole brain radiation and intrathecal Cytosine Arabinoside produced temporary remissions, but both died a few months later of their cancer.", "contents": "Blindness as initial manifestation of meningeal carcinomatosis in breast cancer. The sudden onset of blindness in adults, with or without a history of malignancy should raise the possibility of meningeal carcinomatosis. The diagnosis is best confirmed with examination of the spinal fluid. The actual mechanism by which blindness occurs is probably a combination of tumor cuffing around the optic nerve, direct infiltration and chronic papilledema. We are reporting two patients with disseminated breast cancer who presented with blindness as the first manifestation of meningeal involvement. Treatment with whole brain radiation and intrathecal Cytosine Arabinoside produced temporary remissions, but both died a few months later of their cancer."}
{"id": "PMID:476583", "title": "Prognostic factors in metastatic and hormonally unresponsive carcinoma of the prostate.", "content": "Eighty-eight patients with hormone-resistant Stage IV prostate cancer were treated with a five-drug chemotherapy program. Patient demographic data, prior therapy, symptoms, extent of disease, and laboratory studies were analyzed statistically to evaluate the association of these parameters with survival from the onset of chemotherapy. Factors associated with short survival included age greater than 65, severe bone pain, poor performance status, presence of soft tissue metastases, anemia, elevation of serum LDH, SGOT, alkaline and acid phosphatases, and prolactin, and hypoalbuminemia. Race, stage at initial diagnosis, prior radiation therapy, prior orchiectomy, and elevation of CEA had no prognostic association. We suggest that clinical trials of new therapies of hormone-resistant prostate cancer take into account the presence of these prognostic factors in the analysis of the results of therapeutic programs.", "contents": "Prognostic factors in metastatic and hormonally unresponsive carcinoma of the prostate. Eighty-eight patients with hormone-resistant Stage IV prostate cancer were treated with a five-drug chemotherapy program. Patient demographic data, prior therapy, symptoms, extent of disease, and laboratory studies were analyzed statistically to evaluate the association of these parameters with survival from the onset of chemotherapy. Factors associated with short survival included age greater than 65, severe bone pain, poor performance status, presence of soft tissue metastases, anemia, elevation of serum LDH, SGOT, alkaline and acid phosphatases, and prolactin, and hypoalbuminemia. Race, stage at initial diagnosis, prior radiation therapy, prior orchiectomy, and elevation of CEA had no prognostic association. We suggest that clinical trials of new therapies of hormone-resistant prostate cancer take into account the presence of these prognostic factors in the analysis of the results of therapeutic programs."}
{"id": "PMID:476584", "title": "The thorax as the initial site for systemic relapse in malignant melanoma: a prospective survey of 324 patients.", "content": "Thirteen of 324 patients with malignant melanoma followed during a 24 month period experienced dissemination. The thorax was the initial site for relapse in 12, all of whom were asymptomatic. Ten gave no evidence of extrathoracic disease. Retrospective analysis of previous x-rays originally interpreted as negative revealed metastases in 33%. Life table analysis demonstrated a significantly longer survival for the subset with isolated intrathoracic metastases treated surgically than for their counterparts with metastases no longer amenable to surgery and treated by other modalities. We conclude that the thorax is the site of predilection for initial systemic relapse in malignant melanoma, that detection of early, surgically resectable metastases correlates with longer patient survival, and that routine chest roentgenography is inadequate in reliably uncovering such early disease. These data suggest the potential value of more vigorous radiographic surveillance (with either computed tomography or conventional full lung tomography) in patients at high risk for relapse.", "contents": "The thorax as the initial site for systemic relapse in malignant melanoma: a prospective survey of 324 patients. Thirteen of 324 patients with malignant melanoma followed during a 24 month period experienced dissemination. The thorax was the initial site for relapse in 12, all of whom were asymptomatic. Ten gave no evidence of extrathoracic disease. Retrospective analysis of previous x-rays originally interpreted as negative revealed metastases in 33%. Life table analysis demonstrated a significantly longer survival for the subset with isolated intrathoracic metastases treated surgically than for their counterparts with metastases no longer amenable to surgery and treated by other modalities. We conclude that the thorax is the site of predilection for initial systemic relapse in malignant melanoma, that detection of early, surgically resectable metastases correlates with longer patient survival, and that routine chest roentgenography is inadequate in reliably uncovering such early disease. These data suggest the potential value of more vigorous radiographic surveillance (with either computed tomography or conventional full lung tomography) in patients at high risk for relapse."}
{"id": "PMID:476585", "title": "Leukoerythroblastosis and cancer frequency, prognosis, and physiopathologic significance.", "content": "This investigation was carried out on 100 bone marrow biopsies with metastases and 56 autopsies on patients with evidence of cancer. Leukoerythroblastosis was found in 44% of the patients with bone marrow mestastases and was more frequent in prostatic and gastric carcinoma. Moreover, the postmortem study of patients who died with cancer showed that leukoerythroblastosis was always the sign of bone marrow metastasis. A significant correlation was found between these blood changes and bone marrow fibrosis around the metastasis. Furthermore, leukoerythroblastosis seems caused by hepatosplenic extra medullary hematopoiesis.", "contents": "Leukoerythroblastosis and cancer frequency, prognosis, and physiopathologic significance. This investigation was carried out on 100 bone marrow biopsies with metastases and 56 autopsies on patients with evidence of cancer. Leukoerythroblastosis was found in 44% of the patients with bone marrow mestastases and was more frequent in prostatic and gastric carcinoma. Moreover, the postmortem study of patients who died with cancer showed that leukoerythroblastosis was always the sign of bone marrow metastasis. A significant correlation was found between these blood changes and bone marrow fibrosis around the metastasis. Furthermore, leukoerythroblastosis seems caused by hepatosplenic extra medullary hematopoiesis."}
{"id": "PMID:476587", "title": "Swallowing disorders in three types of head and neck surgical patients.", "content": "This study examined swallowing transit times and motility problems in three groups of patients following ablative surgery for oropharyngeal carcinoma and in a control group of 10 normal subjects. A total of 30 patients was studied: 10 after anterior floor of mouth resection, 12 after tonsil/base of tongue resection, and 8 after supraglottic laryngectomy. Videofluoroscopic studies of liquid, thin paste, thick paste, and thick paste plus liquid swallows were completed 1 week post-initiation of oral feeding following surgery. From the videotapes, oral and pharyngeal transit times were measured, and motility disturbances were defined during each stage of the swallow. All three types of patients in this study showed severe problems with swallowing. The anterior floor of mouth resection patients had problems with preparation for the swallow and oral transit. Tonsil/base of tongue resection patients had slowing in the preparation for the swallow and in the oral and pharyngeal stages. After supraglottic laryngectomy, patients showed only slight slowing in oral transit and pharyngeal transit as compared to other types of surgical patients.", "contents": "Swallowing disorders in three types of head and neck surgical patients. This study examined swallowing transit times and motility problems in three groups of patients following ablative surgery for oropharyngeal carcinoma and in a control group of 10 normal subjects. A total of 30 patients was studied: 10 after anterior floor of mouth resection, 12 after tonsil/base of tongue resection, and 8 after supraglottic laryngectomy. Videofluoroscopic studies of liquid, thin paste, thick paste, and thick paste plus liquid swallows were completed 1 week post-initiation of oral feeding following surgery. From the videotapes, oral and pharyngeal transit times were measured, and motility disturbances were defined during each stage of the swallow. All three types of patients in this study showed severe problems with swallowing. The anterior floor of mouth resection patients had problems with preparation for the swallow and oral transit. Tonsil/base of tongue resection patients had slowing in the preparation for the swallow and in the oral and pharyngeal stages. After supraglottic laryngectomy, patients showed only slight slowing in oral transit and pharyngeal transit as compared to other types of surgical patients."}
{"id": "PMID:476588", "title": "Asbestos, dental X-rays, tobacco, and alcohol in the epidemiology of laryngeal cancer.", "content": "A case-control study of 47 laryngeal cancers in males of three counties of Washington State was conducted. Personal interview was used to obtain information on smoking, alcohol use, exposure to asbestos, and other substances, and x-rays of the head and neck area. Smoking and alcohol consumption were found to increase risk of laryngeal cancer independently, with a clear dose-response relationship. Neither asbestos exposure nor exposure to other substances was found to significantly increase the risk of laryngeal cancer, although the relative risk with asbestos exposure was 1.75. Lifetime history of exposure to dental x-rays on five or more occasions was associated with significantly increased risk of laryngeal cancer among heavy smokers but not among light smokers. The importance of tobacco and alcohol in the epidemiology of laryngeal cancer was re-affirmed, the importance of asbestos exposure was brought into question, and a possible relationship of laryngeal cancer with exposure to dental x-rays among heavy smokers was demonstrated.", "contents": "Asbestos, dental X-rays, tobacco, and alcohol in the epidemiology of laryngeal cancer. A case-control study of 47 laryngeal cancers in males of three counties of Washington State was conducted. Personal interview was used to obtain information on smoking, alcohol use, exposure to asbestos, and other substances, and x-rays of the head and neck area. Smoking and alcohol consumption were found to increase risk of laryngeal cancer independently, with a clear dose-response relationship. Neither asbestos exposure nor exposure to other substances was found to significantly increase the risk of laryngeal cancer, although the relative risk with asbestos exposure was 1.75. Lifetime history of exposure to dental x-rays on five or more occasions was associated with significantly increased risk of laryngeal cancer among heavy smokers but not among light smokers. The importance of tobacco and alcohol in the epidemiology of laryngeal cancer was re-affirmed, the importance of asbestos exposure was brought into question, and a possible relationship of laryngeal cancer with exposure to dental x-rays among heavy smokers was demonstrated."}
{"id": "PMID:476589", "title": "Surgical repair of esophagobronchial fistula following successful treatment of Hodgkin's disease.", "content": "A 33-year-old man with stage III-BS nodular sclerosing Hodgkin's disease received combination chemotherapy and subsequently developed a right middle lobe infiltrate in the lung unresponsive to antibiotic therapy. Thoracotomy revealed an esophagobronchial fistula with no evidence of malignancy. Surgical correction of the fistula was technically difficulty but proved lifesaving for this patient.", "contents": "Surgical repair of esophagobronchial fistula following successful treatment of Hodgkin's disease. A 33-year-old man with stage III-BS nodular sclerosing Hodgkin's disease received combination chemotherapy and subsequently developed a right middle lobe infiltrate in the lung unresponsive to antibiotic therapy. Thoracotomy revealed an esophagobronchial fistula with no evidence of malignancy. Surgical correction of the fistula was technically difficulty but proved lifesaving for this patient."}
{"id": "PMID:476590", "title": "Evaluation of response criteria in advanced lung cancer.", "content": "Because we found it illogical to attempt measurement of nonmeasurable but visible tumors in patients with advanced lung cancer, we devised and used a separate set of response criteria for patients with evaluable, nonmeasurable tumors. Patients with evaluable disease who had obtained a tumor regression after therapy were compared to patients with measurable disease who had obtained a tumor regression according to standard criteria. Among 191 cases evaluated (54% with measurable and 46% with evaluable disease), 59 tumor regressions were found (35 in patients with measurable disease and 24 in patients with evaluable disease). Evaluating regressions in patients with both types of disease, we could not detect statistical differences in regression rates, times to regression, durations of regression or survival. We believe the response criteria used for patients with evaluable, nonmeasurable lung cancer to be valid in assessing response to therapy.", "contents": "Evaluation of response criteria in advanced lung cancer. Because we found it illogical to attempt measurement of nonmeasurable but visible tumors in patients with advanced lung cancer, we devised and used a separate set of response criteria for patients with evaluable, nonmeasurable tumors. Patients with evaluable disease who had obtained a tumor regression after therapy were compared to patients with measurable disease who had obtained a tumor regression according to standard criteria. Among 191 cases evaluated (54% with measurable and 46% with evaluable disease), 59 tumor regressions were found (35 in patients with measurable disease and 24 in patients with evaluable disease). Evaluating regressions in patients with both types of disease, we could not detect statistical differences in regression rates, times to regression, durations of regression or survival. We believe the response criteria used for patients with evaluable, nonmeasurable lung cancer to be valid in assessing response to therapy."}
{"id": "PMID:476591", "title": "Gastric remnant carcinoma.", "content": "One hundred cases of gastric carcinoma at The New York Hospital-Cornell Medical Center included 7 cases of gastric remnant carcinomas, which were analyzed and compared with prior reports in the literature. Gastric remnant carcinoma is a distinct entity, noted in greatest incidence beginning approximately 15 years after surgery for benign gastric disease. Although the etiology is unknown, the high incidence of bile reflux and concomitant existence of histologic change may account for a premalignant environment in which the stomach assumes some absorptive function. Such new activity may result in the influx of potentially carcinogenic compounds and subsequent tumor development. Early diagnosis of remnant carcinoma following gastric surgery is important to improved prognosis, and endoscopic followup is recommended for all such patients beginning 10-15 years postoperatively.", "contents": "Gastric remnant carcinoma. One hundred cases of gastric carcinoma at The New York Hospital-Cornell Medical Center included 7 cases of gastric remnant carcinomas, which were analyzed and compared with prior reports in the literature. Gastric remnant carcinoma is a distinct entity, noted in greatest incidence beginning approximately 15 years after surgery for benign gastric disease. Although the etiology is unknown, the high incidence of bile reflux and concomitant existence of histologic change may account for a premalignant environment in which the stomach assumes some absorptive function. Such new activity may result in the influx of potentially carcinogenic compounds and subsequent tumor development. Early diagnosis of remnant carcinoma following gastric surgery is important to improved prognosis, and endoscopic followup is recommended for all such patients beginning 10-15 years postoperatively."}
{"id": "PMID:476592", "title": "Hemangioendothelial sarcoma of penis.", "content": "Hemangioendothelial sarcoma of the penis in a 44-year-old man was treated by preoperative radiation, penectomy, and chemotherapy. The patient was free of evident disease 2 years later. Electronmicroscopy showed differentiated vascular structures at the periphery of the lesion and anaplastic cells throughout the remainder of the tumor.", "contents": "Hemangioendothelial sarcoma of penis. Hemangioendothelial sarcoma of the penis in a 44-year-old man was treated by preoperative radiation, penectomy, and chemotherapy. The patient was free of evident disease 2 years later. Electronmicroscopy showed differentiated vascular structures at the periphery of the lesion and anaplastic cells throughout the remainder of the tumor."}
{"id": "PMID:476593", "title": "Ureteral obstruction secondary to metastatic breast carcinoma.", "content": "An uncommon manifestation of breast cancer is ureteral obstruction secondary to metastatic disease. Five patients who recently developed this complication from two to 20 years after the diagnosis of breast cancer are described. Only two of the five patients had urinary symptoms. All of the patients were older, postmenopausal females who had bone metastases and all had responded to previous hormonal manipulation. Bone scanning was useful in detecting unsuspected hydronephrosis in two patients. Retroperitoneal disease appears to be a complication of long standing breast cancer which is usually hormonally dependent. Routine examination of the bone scan for renal asymmetry may aid in the diagnosis, especially in asymptomatic patients.", "contents": "Ureteral obstruction secondary to metastatic breast carcinoma. An uncommon manifestation of breast cancer is ureteral obstruction secondary to metastatic disease. Five patients who recently developed this complication from two to 20 years after the diagnosis of breast cancer are described. Only two of the five patients had urinary symptoms. All of the patients were older, postmenopausal females who had bone metastases and all had responded to previous hormonal manipulation. Bone scanning was useful in detecting unsuspected hydronephrosis in two patients. Retroperitoneal disease appears to be a complication of long standing breast cancer which is usually hormonally dependent. Routine examination of the bone scan for renal asymmetry may aid in the diagnosis, especially in asymptomatic patients."}
{"id": "PMID:476594", "title": "Adjuvant chemotherapy of breast cancer.", "content": "One hundred women with primary breast cancer with 4 or more metastatic axillary nodes were treated for 9 months postoperatively with vincristine, prednisone, cyclophosphamide, methotrexate, and fluorouracil (VPCMF). Sixty-five women have been observed for a minimum of 5 years or until failure and the rest for 3 years or more. For 73 women who received adjuvant chemotherapy only, observed for 5 1/2 years median, disease-free status by life table analysis is 68% at 8 years. No significance difference was found between response of pre- and postmenopausal women in disease-free interval or survival. Mortality compared to expectation was sharply reduced; only 9 of 73 have died. These findings demonstrate the long term effectiveness of relatively short-term surgical adjuvant combination chemotherapy in pre- and post-menopausal patients with breast cancer at high risk.", "contents": "Adjuvant chemotherapy of breast cancer. One hundred women with primary breast cancer with 4 or more metastatic axillary nodes were treated for 9 months postoperatively with vincristine, prednisone, cyclophosphamide, methotrexate, and fluorouracil (VPCMF). Sixty-five women have been observed for a minimum of 5 years or until failure and the rest for 3 years or more. For 73 women who received adjuvant chemotherapy only, observed for 5 1/2 years median, disease-free status by life table analysis is 68% at 8 years. No significance difference was found between response of pre- and postmenopausal women in disease-free interval or survival. Mortality compared to expectation was sharply reduced; only 9 of 73 have died. These findings demonstrate the long term effectiveness of relatively short-term surgical adjuvant combination chemotherapy in pre- and post-menopausal patients with breast cancer at high risk."}
{"id": "PMID:476595", "title": "Analysis of proliferative compartments in human tumors. I. Renal adenocarcinoma.", "content": "Vascular perfusion of 16 renal adenocarcinomas with radioactive DNA precursors provides a possibility of characterize proliferative compartments of this tumor type. Immediately after resection of the tumor-bearing kidney, the organ is perfused via renal artery with dextran-diluted, heparinized oxygenated blood at physiological temperature, pH, flow, and pressure in a recirculation system. DNA synthetizing cells are labeled by addition of 3H- or 14C-thymidine or both isotopes at different intervals. Beta camera scans and whole-tumor autoradiograms disclose a striking proliferative heterogeneity of the tumor. Cell proliferation depends on intratumoral localization, cellular differentiation, histological structure and vascular supply. Subpopulations of high proliferative activity are found at the invasive borderline near normal kidney, focally in subcapsular areas and in intrarenal metastases, but also immediately adjacent to necrotic areas in the tumor center. Quantitative evaluation of autoradiograms yields, at the cellular level, a significantly higher labeling index in granular cells (3.21%) than in clear cells (0.65%), with a large variability dependent on the histological structure. The highest number of DNA synthetizing cells is seen in papillary and mixed solid-tubular zones and at peripheral parts of solid areas, whereas in central parts of solid tumor cords and in highly differentiated tubular areas lower labeling indices are observed. The labeling index decreases exponentially as a function of the distance from the supporting blood vessel. In solid cords, no labeled cells are seen at a distance of more than 200 micron from the capillary. The ts determined by 3H/14C-thymidine double labeling is between 9.9 and 16.8 hr for granular cells and about 9.2 hr for clear cells. Potential population doubling time calculated for various subpopulations yields values between 4 and 50 days. It is concluded that cell loss is high, for granular cells in particular. Besides cell loss, a large nonproliferating compartment contributes to a delay of the tumor volume doubling time. Proliferative heterogeneity of advanced human tumors, as exemplified by the renal adenocarcinoma, bears important implications for therapy and prognosis.", "contents": "Analysis of proliferative compartments in human tumors. I. Renal adenocarcinoma. Vascular perfusion of 16 renal adenocarcinomas with radioactive DNA precursors provides a possibility of characterize proliferative compartments of this tumor type. Immediately after resection of the tumor-bearing kidney, the organ is perfused via renal artery with dextran-diluted, heparinized oxygenated blood at physiological temperature, pH, flow, and pressure in a recirculation system. DNA synthetizing cells are labeled by addition of 3H- or 14C-thymidine or both isotopes at different intervals. Beta camera scans and whole-tumor autoradiograms disclose a striking proliferative heterogeneity of the tumor. Cell proliferation depends on intratumoral localization, cellular differentiation, histological structure and vascular supply. Subpopulations of high proliferative activity are found at the invasive borderline near normal kidney, focally in subcapsular areas and in intrarenal metastases, but also immediately adjacent to necrotic areas in the tumor center. Quantitative evaluation of autoradiograms yields, at the cellular level, a significantly higher labeling index in granular cells (3.21%) than in clear cells (0.65%), with a large variability dependent on the histological structure. The highest number of DNA synthetizing cells is seen in papillary and mixed solid-tubular zones and at peripheral parts of solid areas, whereas in central parts of solid tumor cords and in highly differentiated tubular areas lower labeling indices are observed. The labeling index decreases exponentially as a function of the distance from the supporting blood vessel. In solid cords, no labeled cells are seen at a distance of more than 200 micron from the capillary. The ts determined by 3H/14C-thymidine double labeling is between 9.9 and 16.8 hr for granular cells and about 9.2 hr for clear cells. Potential population doubling time calculated for various subpopulations yields values between 4 and 50 days. It is concluded that cell loss is high, for granular cells in particular. Besides cell loss, a large nonproliferating compartment contributes to a delay of the tumor volume doubling time. Proliferative heterogeneity of advanced human tumors, as exemplified by the renal adenocarcinoma, bears important implications for therapy and prognosis."}
{"id": "PMID:476596", "title": "Evaluation of fast neutron teletherapy for advanced carcinomas of the major salivary glands.", "content": "This review analyzes the local control rate of fast neutron beam teletherapy for malignant salivary gland carcinomas compared to conventional photon irradiation at the same institution. Thirty patients with malignant parotid or submaxillary gland tumors were treated with either cobalt-60 (19 patients) or neutrons +/- photons (11 patients). The 19 photon-treated patients were historical controls. No advantage is found for neutrons in patients with tumors less than 3 cm where 100% local control was achieved with either modality. In patients with tumor masses 3-6 cm in size, neutrons demonstrated a possible advantage with a local control rate of 100% (followup, 7-18 months) compared to a control rate of 33% with conventional photon irradiation. Acute treatment-related morbidity was slightly greater in neutron-treated patients, but no long-term complications have been observed to date.", "contents": "Evaluation of fast neutron teletherapy for advanced carcinomas of the major salivary glands. This review analyzes the local control rate of fast neutron beam teletherapy for malignant salivary gland carcinomas compared to conventional photon irradiation at the same institution. Thirty patients with malignant parotid or submaxillary gland tumors were treated with either cobalt-60 (19 patients) or neutrons +/- photons (11 patients). The 19 photon-treated patients were historical controls. No advantage is found for neutrons in patients with tumors less than 3 cm where 100% local control was achieved with either modality. In patients with tumor masses 3-6 cm in size, neutrons demonstrated a possible advantage with a local control rate of 100% (followup, 7-18 months) compared to a control rate of 33% with conventional photon irradiation. Acute treatment-related morbidity was slightly greater in neutron-treated patients, but no long-term complications have been observed to date."}
{"id": "PMID:476598", "title": "MACC chemotherapy for adenocarcinoma and epidermoid carcinoma of the lung: low response rate in a Cooperative Group Study. Eastern Cooperative Oncology Group.", "content": "The MACC (methotrexate, adriamycin, cyclophosphamide, CCNU) regimen was administered to 43 patients with advanced epidermoid and adenocarcinoma of the lung. Only 5 patients (12%), all of whom were ambulatory responded with partial remissions. Median time to progression for the 5 responders was 20 weeks from start of treatment. Median survival was 15.5 weeks for patients with epidermoid cancer and 14.4 weeks for those with adenocarcinoma. Hematologic toxicity was severe, with 2 treatment-related deaths during profound myelosuppression. White blood counts below 2000/microliter were reported in 47%, and below 1,000/microliter in 26%. Since the activity of this regimen, given as it was in full doses, is not superior to that achieved with standard doses of single agents which are less toxic, further employment of the MACC regimen is not recommended, either for advanced disease or as a surgical adjuvant.", "contents": "MACC chemotherapy for adenocarcinoma and epidermoid carcinoma of the lung: low response rate in a Cooperative Group Study. Eastern Cooperative Oncology Group. The MACC (methotrexate, adriamycin, cyclophosphamide, CCNU) regimen was administered to 43 patients with advanced epidermoid and adenocarcinoma of the lung. Only 5 patients (12%), all of whom were ambulatory responded with partial remissions. Median time to progression for the 5 responders was 20 weeks from start of treatment. Median survival was 15.5 weeks for patients with epidermoid cancer and 14.4 weeks for those with adenocarcinoma. Hematologic toxicity was severe, with 2 treatment-related deaths during profound myelosuppression. White blood counts below 2000/microliter were reported in 47%, and below 1,000/microliter in 26%. Since the activity of this regimen, given as it was in full doses, is not superior to that achieved with standard doses of single agents which are less toxic, further employment of the MACC regimen is not recommended, either for advanced disease or as a surgical adjuvant."}
{"id": "PMID:476600", "title": "Malignant peritoneal mesothelioma following radiotherapy for seminoma of the testis.", "content": "The difficulties encountered in establishing a diagnosis of a malignant peritoneal mesothelioma are emphasized in a patient who developed the lesion 16 years after radiation therapy for a seminoma of the testis. Historically, histologically, electron microscopically, and by microincineration, there was no evidence of asbestos exposure. We believe that present lesion may be a consequence of prior radiation therapy.", "contents": "Malignant peritoneal mesothelioma following radiotherapy for seminoma of the testis. The difficulties encountered in establishing a diagnosis of a malignant peritoneal mesothelioma are emphasized in a patient who developed the lesion 16 years after radiation therapy for a seminoma of the testis. Historically, histologically, electron microscopically, and by microincineration, there was no evidence of asbestos exposure. We believe that present lesion may be a consequence of prior radiation therapy."}
{"id": "PMID:476599", "title": "A fatal case of inappropriate ADH secretion induced by cyclophosphamide therapy.", "content": "Cyclophosphamide is used extensively to treat malignancies. A 5-year-old boy with stage IV neuroblastoma is described who developed a fatal syndrome of inappropriate antidiuretic hormone (ADH) secretion after high dose cyclophosphamide therapy.", "contents": "A fatal case of inappropriate ADH secretion induced by cyclophosphamide therapy. Cyclophosphamide is used extensively to treat malignancies. A 5-year-old boy with stage IV neuroblastoma is described who developed a fatal syndrome of inappropriate antidiuretic hormone (ADH) secretion after high dose cyclophosphamide therapy."}
{"id": "PMID:476601", "title": "Neutrophil function in lymphoreticular malignancy.", "content": "Neutrophil chemotaxis, phagocytosis, and metabolic stimulation were studied in 25 patients with untreated lymphoreticular malignancies, 8 patients with untreated carcinomas, and 8 patients undergoing therapy for lymphoreticular tumors. Phagocytic activity and metabolic stimulation during phagocytosis were comparable to controls in all patient groups. Slight, but significant, differences were found in chemotactic responsiveness of untreated patient groups when compared to controls. In almost all instances, chemotactic responsiveness reverted to normal in most patients studied after therapy for the malignant process. In patients with intact immunologic function these minor differences in chemotactic responsiveness are probably not sufficient to predispose to infection. However, this defect could be additive, and when present in combination with impaired immunity may contribute to compromised host defense.", "contents": "Neutrophil function in lymphoreticular malignancy. Neutrophil chemotaxis, phagocytosis, and metabolic stimulation were studied in 25 patients with untreated lymphoreticular malignancies, 8 patients with untreated carcinomas, and 8 patients undergoing therapy for lymphoreticular tumors. Phagocytic activity and metabolic stimulation during phagocytosis were comparable to controls in all patient groups. Slight, but significant, differences were found in chemotactic responsiveness of untreated patient groups when compared to controls. In almost all instances, chemotactic responsiveness reverted to normal in most patients studied after therapy for the malignant process. In patients with intact immunologic function these minor differences in chemotactic responsiveness are probably not sufficient to predispose to infection. However, this defect could be additive, and when present in combination with impaired immunity may contribute to compromised host defense."}
{"id": "PMID:476602", "title": "Histopathologic changes of nasal mucosa in nickel workers: a pilot study.", "content": "Histologic examination of standard biopsy specimens from nasal mucosa were performed on 98 former and present employees at a nickel refinery and 61 controls. Histologic characteristics of the epithelium were graded blindly according to a point score table. The histologic scores were compared to certain epidemiologic data, such as the degree and duration of nickel exposure, duration of industrial work without nickel exposure, age, and smoking habits. High histologic scores were correlated to high nickel exposure, to the duration of this exposure, to age, and to industrial work not involved with nickel. One person with nasal carcinoma was detected in the nickel-exposed group. During the investigation period, three additional nasal carcinomas in former nickel workers were discovered and later included in the study. Furthermore, 18 cases of epithelial dysplasia were found in the nickelexposed group, but none among the controls. The cases with nasal carcinomas were or had been working with processes that gave an estimated high nickel dust exposure. All the individuals with epithelial dysplasia were nickel process workers.", "contents": "Histopathologic changes of nasal mucosa in nickel workers: a pilot study. Histologic examination of standard biopsy specimens from nasal mucosa were performed on 98 former and present employees at a nickel refinery and 61 controls. Histologic characteristics of the epithelium were graded blindly according to a point score table. The histologic scores were compared to certain epidemiologic data, such as the degree and duration of nickel exposure, duration of industrial work without nickel exposure, age, and smoking habits. High histologic scores were correlated to high nickel exposure, to the duration of this exposure, to age, and to industrial work not involved with nickel. One person with nasal carcinoma was detected in the nickel-exposed group. During the investigation period, three additional nasal carcinomas in former nickel workers were discovered and later included in the study. Furthermore, 18 cases of epithelial dysplasia were found in the nickelexposed group, but none among the controls. The cases with nasal carcinomas were or had been working with processes that gave an estimated high nickel dust exposure. All the individuals with epithelial dysplasia were nickel process workers."}
{"id": "PMID:476603", "title": "Noninvasive precursor lesions of adenocarcinoma and mixed adenosquamous carcinoma of the cervix uteri.", "content": "It is postulated that squamous cell carcinoma, adenocarcinoma, and mixed adenosquamous cell carcinoma of the uterine cervix all have a common cell or origin, the subcolumnar reserve cell. The relative frequency with which the various types of carcinoma in situ are seen is in part explained by the ubiquitous nature of squamous metaplasia in the region of the transformation zone in women of reproductive age. It is suggested that squamous metaplasia is the soil on which most squamous carcinomas of the cervix evolve. The relatively low frequency with which adenocarcinoma and adenosquamous carcinoma in situ are encountered may also be dependent on their less accessible location in the endocervix.", "contents": "Noninvasive precursor lesions of adenocarcinoma and mixed adenosquamous carcinoma of the cervix uteri. It is postulated that squamous cell carcinoma, adenocarcinoma, and mixed adenosquamous cell carcinoma of the uterine cervix all have a common cell or origin, the subcolumnar reserve cell. The relative frequency with which the various types of carcinoma in situ are seen is in part explained by the ubiquitous nature of squamous metaplasia in the region of the transformation zone in women of reproductive age. It is suggested that squamous metaplasia is the soil on which most squamous carcinomas of the cervix evolve. The relatively low frequency with which adenocarcinoma and adenosquamous carcinoma in situ are encountered may also be dependent on their less accessible location in the endocervix."}
{"id": "PMID:476604", "title": "Synergistic effect of diethylstilbestrol on the mutagenicity of 2-acetylaminofluorene and N-hydroxy-acetylaminofluorene in the Salmonella assay system.", "content": "Salmonella typhimurium, TA-1538, was used to investigate the mutagenic potential of N-2-acetylaminofluorene (2-AAF), N-hydroxy-N-2-acetylaminofluorene (N-OH-2-AAF) and diethylstilbestrol (DES) individual and in combination. In the presence of an induced or uninduced rat liver metabolizing system (S-9), the histidine requiring strain of bacteria was reverted to prototrophy by the aromatic amines but not by the synthetic estrogen. However, when DES was combined with 2-AAF or N-OH-I-AAF in the presence of the induced S-9 fraction, the number of revertant colonies was increased 2- to 4-fold above the levels obtained with the aromatic amines alone. The synergistic effect of DES, a non-mutagen, on the mutagenicity of these aromatic amines was observed only when a 3-methylcholanthrene induced rat liver S-9 fraction was used as the source of mammalian enzymes. When uninduced mouse or rat liver S-9 fractions were used in this test system, an inhibitory effect rather than an enhancing effect was observed.", "contents": "Synergistic effect of diethylstilbestrol on the mutagenicity of 2-acetylaminofluorene and N-hydroxy-acetylaminofluorene in the Salmonella assay system. Salmonella typhimurium, TA-1538, was used to investigate the mutagenic potential of N-2-acetylaminofluorene (2-AAF), N-hydroxy-N-2-acetylaminofluorene (N-OH-2-AAF) and diethylstilbestrol (DES) individual and in combination. In the presence of an induced or uninduced rat liver metabolizing system (S-9), the histidine requiring strain of bacteria was reverted to prototrophy by the aromatic amines but not by the synthetic estrogen. However, when DES was combined with 2-AAF or N-OH-I-AAF in the presence of the induced S-9 fraction, the number of revertant colonies was increased 2- to 4-fold above the levels obtained with the aromatic amines alone. The synergistic effect of DES, a non-mutagen, on the mutagenicity of these aromatic amines was observed only when a 3-methylcholanthrene induced rat liver S-9 fraction was used as the source of mammalian enzymes. When uninduced mouse or rat liver S-9 fractions were used in this test system, an inhibitory effect rather than an enhancing effect was observed."}
{"id": "PMID:476605", "title": "Reactions of N-methyl-N-nitrosobenzylamine and related substrates with enzyme-containing cell fractions isolated from various organs of rats and mice.", "content": "Incubation of the following N-nitroso compounds with microsomes from mouse liver and an NADPH-generating system formed the products stated: benzaldehyde from N-methyl-N-nitrosobenzylamine (MNBA), acetone and formaldehyde from N-methyl-N-nitroso-(alpha-phenyl)-ethylamine (MNPE) and formaldehyde from N-methyl-N-nitroso-(2-phenyl)-isopropylamine (MNPI). Enzymes isolated from mouse liver showed more activity towards MNBA by comparison with rat liver preparations. Microsomes from the organs of female rats showed decreasing activity in the following sequence: esophagus greater than forestomach greater than liver and for female mice: liver greater than forestomach.", "contents": "Reactions of N-methyl-N-nitrosobenzylamine and related substrates with enzyme-containing cell fractions isolated from various organs of rats and mice. Incubation of the following N-nitroso compounds with microsomes from mouse liver and an NADPH-generating system formed the products stated: benzaldehyde from N-methyl-N-nitrosobenzylamine (MNBA), acetone and formaldehyde from N-methyl-N-nitroso-(alpha-phenyl)-ethylamine (MNPE) and formaldehyde from N-methyl-N-nitroso-(2-phenyl)-isopropylamine (MNPI). Enzymes isolated from mouse liver showed more activity towards MNBA by comparison with rat liver preparations. Microsomes from the organs of female rats showed decreasing activity in the following sequence: esophagus greater than forestomach greater than liver and for female mice: liver greater than forestomach."}
{"id": "PMID:476606", "title": "Epidermal prostaglandins after topical application of a tumor promotor.", "content": "The topical application of the promoter, 12-O-tetradecanoyl-13-acetate, on the shaved backs of mice was attended by a striking increase in the PGE concentration within epidermis as early as 1 h later. This increase peaked at 24 h but the prostaglandin value remained elevated for at least 48 h. PGF, on the other hand, showed only a modest increase in epidermis with a maximum at 12 h. By 48 h, the PGF concentration returned to normal.", "contents": "Epidermal prostaglandins after topical application of a tumor promotor. The topical application of the promoter, 12-O-tetradecanoyl-13-acetate, on the shaved backs of mice was attended by a striking increase in the PGE concentration within epidermis as early as 1 h later. This increase peaked at 24 h but the prostaglandin value remained elevated for at least 48 h. PGF, on the other hand, showed only a modest increase in epidermis with a maximum at 12 h. By 48 h, the PGF concentration returned to normal."}
{"id": "PMID:476607", "title": "The morphologic and biologic patterns of chemically induced pancreatic adenocarcinoma in Syrian golden hamsters after homologous transplantation.", "content": "A pancreatic adenocarcinoma induced by N-nitrosobis(2-oxopropyl)amine in the Syrian golden hamster was successfully transplanted to a homologous host by subcutaneous inoculation through 10 successive passages. The rate of 'tumor take' increased progressively with each generation from 60% to 100%, and the latency period after inoculation was reduced simultaneously from 6 weeks to 1 week in the second and following passages. The tumors grew rapidly, ulcerated the overlying skin, and metastasized to the regional lymph nodes and lungs. The animals usually died with multiple lung metastases between the 5th and 20th weeks. All transplanted tumors and their metastases retained the pattern of the original, well-differentiated adenocarcinomas.", "contents": "The morphologic and biologic patterns of chemically induced pancreatic adenocarcinoma in Syrian golden hamsters after homologous transplantation. A pancreatic adenocarcinoma induced by N-nitrosobis(2-oxopropyl)amine in the Syrian golden hamster was successfully transplanted to a homologous host by subcutaneous inoculation through 10 successive passages. The rate of 'tumor take' increased progressively with each generation from 60% to 100%, and the latency period after inoculation was reduced simultaneously from 6 weeks to 1 week in the second and following passages. The tumors grew rapidly, ulcerated the overlying skin, and metastasized to the regional lymph nodes and lungs. The animals usually died with multiple lung metastases between the 5th and 20th weeks. All transplanted tumors and their metastases retained the pattern of the original, well-differentiated adenocarcinomas."}
{"id": "PMID:476608", "title": "Mutagenicity of N-nitroso pyridinol carbamate with the Salmonella/mammalian microsome test.", "content": "Pyridinol carbamate was nitrosated to the N,N1-dinitroso derivative and the structure was proved by spectroscopic methods, including chemical ionization mass spectroscopy. By the Ames test, the dinitroso derivative showed a significant dose-dependent mutagenic response with Salmonella typhimurium strains TA 1535 and TA 100; the response became more pronounced in the presence of microsomes. As the dosage of N-nitroso pyridinol carbamate increased, the number of revertant colonies also increased. Pyridinol carbamate was not mutagenic.", "contents": "Mutagenicity of N-nitroso pyridinol carbamate with the Salmonella/mammalian microsome test. Pyridinol carbamate was nitrosated to the N,N1-dinitroso derivative and the structure was proved by spectroscopic methods, including chemical ionization mass spectroscopy. By the Ames test, the dinitroso derivative showed a significant dose-dependent mutagenic response with Salmonella typhimurium strains TA 1535 and TA 100; the response became more pronounced in the presence of microsomes. As the dosage of N-nitroso pyridinol carbamate increased, the number of revertant colonies also increased. Pyridinol carbamate was not mutagenic."}
{"id": "PMID:476609", "title": "Differential excision from DNA of the C-8 deoxyguanosine reaction products of N-hydroxy-2-aminofluorene and N-acetoxy-N-acetyl-2-aminofluorene by endonuclease S1 from Aspergillus oryzae.", "content": "Calf thymus DNA was modified in vitro with [G-3H]N-hydroxy-2-aminofluorene and [G-3H]N-acetoxy-N-acetyl-2-aminofluorene and the nuclease S1 digestion was studied under identical conditions. The ratios of the maximum reaction rate (V) and the Michaelis constant (Km), V/Km, indicate that 2-aminofluorene(AF)-modified DNA is hydrolyzed 3 times more slowly than N-acetyl-2-aminofluorene(AAF)-modified DNA under similar reaction conditions. The AF-modified DNA was slightly more susceptible to partial digestion by nuclease S1 than unmodified control DNA. These results suggest that the local regions of denaturation induced by AF substitution are smaller than those associated with AAF modification.", "contents": "Differential excision from DNA of the C-8 deoxyguanosine reaction products of N-hydroxy-2-aminofluorene and N-acetoxy-N-acetyl-2-aminofluorene by endonuclease S1 from Aspergillus oryzae. Calf thymus DNA was modified in vitro with [G-3H]N-hydroxy-2-aminofluorene and [G-3H]N-acetoxy-N-acetyl-2-aminofluorene and the nuclease S1 digestion was studied under identical conditions. The ratios of the maximum reaction rate (V) and the Michaelis constant (Km), V/Km, indicate that 2-aminofluorene(AF)-modified DNA is hydrolyzed 3 times more slowly than N-acetyl-2-aminofluorene(AAF)-modified DNA under similar reaction conditions. The AF-modified DNA was slightly more susceptible to partial digestion by nuclease S1 than unmodified control DNA. These results suggest that the local regions of denaturation induced by AF substitution are smaller than those associated with AAF modification."}
{"id": "PMID:476610", "title": "Aryl hydrocarbon hydroxylase induction in mitogen-stimulated lymphocytes by benzanthracene or cigarette tars adsorbed to asbestos fibers.", "content": "Human mitogen-stimulated lymphocytes, cultured in the presence of amosite asbestos (AS), demonstrated a slight increase in aryl hydrocarbon hydroxylase (AHH) activity compared with non-induced (control) cultures (P = 0.005). A much greater increase in enzyme activity occurred following addition of the inducers benzanthracene (BA) or cigarette tars (CT) to cell cultures (P less than 0.001 in both instances). Significant enzyme induction also occurred when AS fibers were first preincubated with CT or BA, washed with acetone, then added to lymphocyte cultures (P less than 0.003 in all instances). This increase in AHH activity was not as great, however, as the induction observed when BA or CT was added to cell cultures. No further increase in enzyme activity was noted when AS and CT or AS and BA were simultaneously added to culture lymphocytes (P greater than 0.070 in all instances). The results demonstrate that polycyclic aromatic hydrocarbons (PAH), such as BA and other components of CT, are adsorbed and transported by amosite AS particles. These AS-PAH complexes are capable of inducing AHH in cultured human lymphocytes.", "contents": "Aryl hydrocarbon hydroxylase induction in mitogen-stimulated lymphocytes by benzanthracene or cigarette tars adsorbed to asbestos fibers. Human mitogen-stimulated lymphocytes, cultured in the presence of amosite asbestos (AS), demonstrated a slight increase in aryl hydrocarbon hydroxylase (AHH) activity compared with non-induced (control) cultures (P = 0.005). A much greater increase in enzyme activity occurred following addition of the inducers benzanthracene (BA) or cigarette tars (CT) to cell cultures (P less than 0.001 in both instances). Significant enzyme induction also occurred when AS fibers were first preincubated with CT or BA, washed with acetone, then added to lymphocyte cultures (P less than 0.003 in all instances). This increase in AHH activity was not as great, however, as the induction observed when BA or CT was added to cell cultures. No further increase in enzyme activity was noted when AS and CT or AS and BA were simultaneously added to culture lymphocytes (P greater than 0.070 in all instances). The results demonstrate that polycyclic aromatic hydrocarbons (PAH), such as BA and other components of CT, are adsorbed and transported by amosite AS particles. These AS-PAH complexes are capable of inducing AHH in cultured human lymphocytes."}
{"id": "PMID:476611", "title": "Effects of temperature and time on mutagen formation in pan-fried hamburger.", "content": "Mutagenic activity generated in hamburger during pan-frying is dependent upon both temperature and time, with temperature appearing to be the more important variable. Uniformly prepared frozen hamburger pattie (115 g; 19% fat) were fried under carefully controlled conditions at 143 degrees C, 191 degrees C and 210 degrees C. Mutagenic activity assayed with the Ames test was not detected in uncooked hamburger, and in hamburger fried at 143 degrees C mutagenic activity remained low at all times studied (4--20 min). In contrast, frying at 191 degrees C or 210 degrees C for up to 10 min resulted in the generation of considerably higher levels of mutagenic activity. Mutagenic activity in fried hamburgers sold at selected restaurants ranged from very low to moderately high. Evidence is also presented for mutagenic inhibitory activity in uncooked and fried hamburger. Mutagenic inhibitory activity decreased mutagenesis mediated by liver S-9 from normal rats but not from Aroclor 1254-treated rats.", "contents": "Effects of temperature and time on mutagen formation in pan-fried hamburger. Mutagenic activity generated in hamburger during pan-frying is dependent upon both temperature and time, with temperature appearing to be the more important variable. Uniformly prepared frozen hamburger pattie (115 g; 19% fat) were fried under carefully controlled conditions at 143 degrees C, 191 degrees C and 210 degrees C. Mutagenic activity assayed with the Ames test was not detected in uncooked hamburger, and in hamburger fried at 143 degrees C mutagenic activity remained low at all times studied (4--20 min). In contrast, frying at 191 degrees C or 210 degrees C for up to 10 min resulted in the generation of considerably higher levels of mutagenic activity. Mutagenic activity in fried hamburgers sold at selected restaurants ranged from very low to moderately high. Evidence is also presented for mutagenic inhibitory activity in uncooked and fried hamburger. Mutagenic inhibitory activity decreased mutagenesis mediated by liver S-9 from normal rats but not from Aroclor 1254-treated rats."}
{"id": "PMID:476612", "title": "High incidence of rat mammary carcinoma by oral administration of ethyl methanesulphonate.", "content": "Ethyl methanesulphonate (EMS), one of the alkylating agents, is known to be a potent mutagen. We tested EMS for its carcinogenicity in female rats by oral administration. EMS was dissolved in drinking tap water at a concentration of 10(-3) M and was given for the first 12 weeks. The subcutaneous tumors were noticed as early as 16 weeks after initiating the experiment. At the 32nd week, all the surviving rats produced tumors; the majority were multiple tumors in the neck, axillar and inguinal areas corresponding to bilateral mammary glands. Histologically, the prevailing feature of the tumors was infiltrating medullary adenocarcinoma consistent with carcinoma of mammary duct origin. Neither regional lymph node involvement nor distant metastases were shown, but intraductal spread of carcinoma was a marked finding during the 32-week period.", "contents": "High incidence of rat mammary carcinoma by oral administration of ethyl methanesulphonate. Ethyl methanesulphonate (EMS), one of the alkylating agents, is known to be a potent mutagen. We tested EMS for its carcinogenicity in female rats by oral administration. EMS was dissolved in drinking tap water at a concentration of 10(-3) M and was given for the first 12 weeks. The subcutaneous tumors were noticed as early as 16 weeks after initiating the experiment. At the 32nd week, all the surviving rats produced tumors; the majority were multiple tumors in the neck, axillar and inguinal areas corresponding to bilateral mammary glands. Histologically, the prevailing feature of the tumors was infiltrating medullary adenocarcinoma consistent with carcinoma of mammary duct origin. Neither regional lymph node involvement nor distant metastases were shown, but intraductal spread of carcinoma was a marked finding during the 32-week period."}
{"id": "PMID:476613", "title": "Enhancement of experimental photocarcinogenesis by topical retinoic acid.", "content": "Topical application of retinoic acid (RA) solutions greatly enhanced the response of hairless mouse skin to a moderate dose of simulated sunlight. Tumors appeared much earlier, and in much greater numbers, in animals treated daily with 1 or 10 micrograms of RA in methanol immediately after 2 h exposure to a xenon arc filtered through 2 mm of Schott WG 320 glass (approximately equivalent in human erythema effectiveness to 5 min of mid-summer noon solar exposure in northern mid-latitudes), compared to mice treated with light and methanol only. The higher amount of RA, in combination with light, produced moderate epidermal hyperplasia and some scaling and transient erythema, but no gross ulceration or inflammation of skin. The lower amount of RA, though about equally effective in carcinogenesis, produced minimal epidermal hyperplasia compared to the ultraviolet radiation + methanol control.", "contents": "Enhancement of experimental photocarcinogenesis by topical retinoic acid. Topical application of retinoic acid (RA) solutions greatly enhanced the response of hairless mouse skin to a moderate dose of simulated sunlight. Tumors appeared much earlier, and in much greater numbers, in animals treated daily with 1 or 10 micrograms of RA in methanol immediately after 2 h exposure to a xenon arc filtered through 2 mm of Schott WG 320 glass (approximately equivalent in human erythema effectiveness to 5 min of mid-summer noon solar exposure in northern mid-latitudes), compared to mice treated with light and methanol only. The higher amount of RA, in combination with light, produced moderate epidermal hyperplasia and some scaling and transient erythema, but no gross ulceration or inflammation of skin. The lower amount of RA, though about equally effective in carcinogenesis, produced minimal epidermal hyperplasia compared to the ultraviolet radiation + methanol control."}
{"id": "PMID:476614", "title": "Further studies of the effect of pepstatin on ascites accumulation in tumor-bearing mice.", "content": "Studies were done of the effect of pepstatin on ascites accumulation in mice bearing MM46, Ehrlich, CCM, SN36, L1210, and NTF ascites tumors. When pepstatin was injected subcutaneously at 80 mg/kg body wt before ascites accumulation, it inhibited the accumulation in all strains of the tumors tested. In MM46, CCM and NTF tumor strains there was also a decrease in the tumor cell numbers following pepstatin treatment. Kinetic studies on ascites accumulation with tumor strain MM46 demonstrated that even when pepstatin was injected after ascites accumulation it reduced the ascites volume. A dose-dependent effect was observed in this tumor strain when pepstatin was injected both before and after ascites accumulation. The results confirm previous studies of pepstatin's ability to retard ascites in L1210 and P-815Y ascites tumors and also broaden the concept of the mechanisms by which petstatin may be acting.", "contents": "Further studies of the effect of pepstatin on ascites accumulation in tumor-bearing mice. Studies were done of the effect of pepstatin on ascites accumulation in mice bearing MM46, Ehrlich, CCM, SN36, L1210, and NTF ascites tumors. When pepstatin was injected subcutaneously at 80 mg/kg body wt before ascites accumulation, it inhibited the accumulation in all strains of the tumors tested. In MM46, CCM and NTF tumor strains there was also a decrease in the tumor cell numbers following pepstatin treatment. Kinetic studies on ascites accumulation with tumor strain MM46 demonstrated that even when pepstatin was injected after ascites accumulation it reduced the ascites volume. A dose-dependent effect was observed in this tumor strain when pepstatin was injected both before and after ascites accumulation. The results confirm previous studies of pepstatin's ability to retard ascites in L1210 and P-815Y ascites tumors and also broaden the concept of the mechanisms by which petstatin may be acting."}
{"id": "PMID:476615", "title": "Comparison of the skin tumor initiating activity of 3-methylcholanthrene and 3,11-dimethylcholanthrene in mice.", "content": "The abilities of 3-methylcholanthrene (3-MC) and 3,11-dimethylcholanthrene (3,11-DMC) to initiate skin tumors in Sencar mice were determined by using a 2-stage system of tumorigenesis. 3,11-DMC was found to have very weak skin tumor initiating activity when compared to the potent activity of 3-MC. The only difference between 3-MC and 3,11-DMC is the substitution of a methyl group in position 11 which is part of the 'K-region' or the 'peri' position. From these results, we suggest that an unhindered peri position adjacent to an angular benzene ring is necessary for carcinogenic activity of 3-MC.", "contents": "Comparison of the skin tumor initiating activity of 3-methylcholanthrene and 3,11-dimethylcholanthrene in mice. The abilities of 3-methylcholanthrene (3-MC) and 3,11-dimethylcholanthrene (3,11-DMC) to initiate skin tumors in Sencar mice were determined by using a 2-stage system of tumorigenesis. 3,11-DMC was found to have very weak skin tumor initiating activity when compared to the potent activity of 3-MC. The only difference between 3-MC and 3,11-DMC is the substitution of a methyl group in position 11 which is part of the 'K-region' or the 'peri' position. From these results, we suggest that an unhindered peri position adjacent to an angular benzene ring is necessary for carcinogenic activity of 3-MC."}
{"id": "PMID:476617", "title": "Effect of methylprednisolone on the cell kinetic response of C3H/HeJ mammary tumors to cyclophosphamide and adriamycin.", "content": "The effect of methylprednisolone (MP) on the cell kinetic response to cyclophosphamide (CP) and Adriamycin (ADR) in C3H/HeJ spontaneous mammary tumors and hematopoietic tissue was investigated. The [3H]deoxythymidine labelingg index, the primer-dependent DNA polymerase labeling index (an estimate of tumor growth fraction), and the mitotic index were determined at various intervals after treatment. Treatment consisted of CP (200 mg/kg) on Day 0 plus ADR (2 mg/kg) on Day 1 with or without MP every 12 hr for 9 doses beginning on Day 2. In tumors treated with CP and ADR alone, changes in the kinetic parameters suggested proliferative recovery between Days 3 and 4 which coincided with bone marrow recovery. In tumors treated with CP, ADR, and MP, although the timing of the hematopoietic recovery was not affected by MP, the overshoot of the [3H]deoxythymidine labelin index on Days 3 and 4 was abolished. Proliferative recovery in the tumor was delayed until after cessation of MP treatments. Cell kinetic changes in the tumor after CP, ADR, and MP were used to design effective sequential chemotherapy which obviated the hematopoietic toxicity associated with sequential therapy designed from cell kinetic changes after CP and ADR alone.", "contents": "Effect of methylprednisolone on the cell kinetic response of C3H/HeJ mammary tumors to cyclophosphamide and adriamycin. The effect of methylprednisolone (MP) on the cell kinetic response to cyclophosphamide (CP) and Adriamycin (ADR) in C3H/HeJ spontaneous mammary tumors and hematopoietic tissue was investigated. The [3H]deoxythymidine labelingg index, the primer-dependent DNA polymerase labeling index (an estimate of tumor growth fraction), and the mitotic index were determined at various intervals after treatment. Treatment consisted of CP (200 mg/kg) on Day 0 plus ADR (2 mg/kg) on Day 1 with or without MP every 12 hr for 9 doses beginning on Day 2. In tumors treated with CP and ADR alone, changes in the kinetic parameters suggested proliferative recovery between Days 3 and 4 which coincided with bone marrow recovery. In tumors treated with CP, ADR, and MP, although the timing of the hematopoietic recovery was not affected by MP, the overshoot of the [3H]deoxythymidine labelin index on Days 3 and 4 was abolished. Proliferative recovery in the tumor was delayed until after cessation of MP treatments. Cell kinetic changes in the tumor after CP, ADR, and MP were used to design effective sequential chemotherapy which obviated the hematopoietic toxicity associated with sequential therapy designed from cell kinetic changes after CP and ADR alone."}
{"id": "PMID:476618", "title": "Influence of immune status on the metastasis of three murine fibrosarcomas of different immunogenicities.", "content": "Three fibrosarcomas of different immunogenicities were tested for their ability to form spontaneous and experimentally induced metastases in normal, sham-suppressed, immunosuppressed, and immunologically restored syngeneic mice. Immunosuppression, achieved by adult thymectomy and sublethal X-irradiation (450 R), affected experimental metastasis of the three tumors in different ways. Upon i.v. injection, the highly immunogenic fibrosarcoma formed more pulmonary tumor colonies in immunosuppressed mice than in normal, sham-suppressed (sham thymectomy and 450 R), or immunologically reconstituted animals (thymectomy, X-irradiation, plus 10(7) normal syngeneic lymphocytes given i.v.). A fibrosarcoma of intermediate immunogenicity also formed more pulmonary metastases in immunosuppressed recipients, but this increase could not be reversed by reconstitution with 10(7) lymphocytes. In contrast, the least immunogenic tumor formed fewer pulmonary tumor colonies in immunosuppressed mice than in normal, sham-suppressed, or immunologically reconstituted mice. We conclude that the role of the immune system in experimental cancer metastasis varies for different tumors and that tumor immunogenicity is an important factor in the relationship between host immunity and tumor dissemination.", "contents": "Influence of immune status on the metastasis of three murine fibrosarcomas of different immunogenicities. Three fibrosarcomas of different immunogenicities were tested for their ability to form spontaneous and experimentally induced metastases in normal, sham-suppressed, immunosuppressed, and immunologically restored syngeneic mice. Immunosuppression, achieved by adult thymectomy and sublethal X-irradiation (450 R), affected experimental metastasis of the three tumors in different ways. Upon i.v. injection, the highly immunogenic fibrosarcoma formed more pulmonary tumor colonies in immunosuppressed mice than in normal, sham-suppressed (sham thymectomy and 450 R), or immunologically reconstituted animals (thymectomy, X-irradiation, plus 10(7) normal syngeneic lymphocytes given i.v.). A fibrosarcoma of intermediate immunogenicity also formed more pulmonary metastases in immunosuppressed recipients, but this increase could not be reversed by reconstitution with 10(7) lymphocytes. In contrast, the least immunogenic tumor formed fewer pulmonary tumor colonies in immunosuppressed mice than in normal, sham-suppressed, or immunologically reconstituted mice. We conclude that the role of the immune system in experimental cancer metastasis varies for different tumors and that tumor immunogenicity is an important factor in the relationship between host immunity and tumor dissemination."}
{"id": "PMID:476619", "title": "Synergistic action of 5-aza-2'-deoxycytidine and 3-deazauridine on L1210 leukemic cells and EMT6 tumor cells.", "content": "The biochemical and biological effects of the combination of 5-aza-2'-deoxycytidine (5-aza-dCyd) and 3-deazauridine (3-DU) on L1210 leukemic cells and EMT6 tumor cells were investigated. The cytotoxic action of 5-aza-dCyd and 3-DU on both L1210 and EMT6 cells in vitro was synergistic when these agents were used in combination. The combination of 5-aza-dCyd and 3-DU produced a greater inhibition of in vitro growth of L1210 and EMT6 cells than did either agent alone. The in vivo antineoplastic activity of this combination was synergistic with respect to the increased survival time of BALB/c x DBA/2 F1 mice with L1210 leukemia. 3-DU, an agent that reduces the intracellular pool size of cytosine nucleotides, stimulated the incorporation of [3H]-5-aza-dCyd into DNA of both L1210 and EMT6 cells, suggesting that the synergistic action of this combination is related to the increased incorporation of 5-aza-dCyd in the presence of 3-DU.", "contents": "Synergistic action of 5-aza-2'-deoxycytidine and 3-deazauridine on L1210 leukemic cells and EMT6 tumor cells. The biochemical and biological effects of the combination of 5-aza-2'-deoxycytidine (5-aza-dCyd) and 3-deazauridine (3-DU) on L1210 leukemic cells and EMT6 tumor cells were investigated. The cytotoxic action of 5-aza-dCyd and 3-DU on both L1210 and EMT6 cells in vitro was synergistic when these agents were used in combination. The combination of 5-aza-dCyd and 3-DU produced a greater inhibition of in vitro growth of L1210 and EMT6 cells than did either agent alone. The in vivo antineoplastic activity of this combination was synergistic with respect to the increased survival time of BALB/c x DBA/2 F1 mice with L1210 leukemia. 3-DU, an agent that reduces the intracellular pool size of cytosine nucleotides, stimulated the incorporation of [3H]-5-aza-dCyd into DNA of both L1210 and EMT6 cells, suggesting that the synergistic action of this combination is related to the increased incorporation of 5-aza-dCyd in the presence of 3-DU."}
{"id": "PMID:476620", "title": "Suppression of tumor-associated hyperfibrinogenemia and free fatty acidemia with p-phenoxybenzalbutyrate (clofibrate).", "content": "Previous work in our laboratory has indicated that free fatty acids stimulate synthesis of fibrinogen by the liver. The effect of the hypolipidemic agent clofibrate on hyperfibrinogenemia associated with tumors was evaluated by monitoring clofibrate-induced changes in plasma fibrinogen concentration and biosynthesis of the protein in Buffalo rats implanted with a localized, nonmetastasizing neoplasm derived from a tumorigenic hepatoma cell line (HTC4). In tumor-bearing animals not treated with clofibrate, cancer growth was associated with elevated rates of fibrinogen synthesis and a doubling of plasma fibrinogen concentrations. Plasma free fatty acid concentrations and serum free fatty acid/albumin molar ratios were also increased in tumor-bearing rats. Treatment with clofibrate in doses which normalized the plasma free fatty acid/albumin ratio also prevented the tumor-associated rise in plasma fibrinogen. Rates of fibrinogen synthesis were lowered significantly in clofibrate-treated animals. Tumor growth was not affected by clofibrate. These results indicate that hyperfibrinogenemia associated with nonmetastasizing tumors may reflect changes in lipid metabolism which are neutralized by clofibrate. Thus, treatment with clofibrate or other hypolipidemic agents should be evaluated in cancer patients with elevated plasma fibrinogen levels and their attendant complications.", "contents": "Suppression of tumor-associated hyperfibrinogenemia and free fatty acidemia with p-phenoxybenzalbutyrate (clofibrate). Previous work in our laboratory has indicated that free fatty acids stimulate synthesis of fibrinogen by the liver. The effect of the hypolipidemic agent clofibrate on hyperfibrinogenemia associated with tumors was evaluated by monitoring clofibrate-induced changes in plasma fibrinogen concentration and biosynthesis of the protein in Buffalo rats implanted with a localized, nonmetastasizing neoplasm derived from a tumorigenic hepatoma cell line (HTC4). In tumor-bearing animals not treated with clofibrate, cancer growth was associated with elevated rates of fibrinogen synthesis and a doubling of plasma fibrinogen concentrations. Plasma free fatty acid concentrations and serum free fatty acid/albumin molar ratios were also increased in tumor-bearing rats. Treatment with clofibrate in doses which normalized the plasma free fatty acid/albumin ratio also prevented the tumor-associated rise in plasma fibrinogen. Rates of fibrinogen synthesis were lowered significantly in clofibrate-treated animals. Tumor growth was not affected by clofibrate. These results indicate that hyperfibrinogenemia associated with nonmetastasizing tumors may reflect changes in lipid metabolism which are neutralized by clofibrate. Thus, treatment with clofibrate or other hypolipidemic agents should be evaluated in cancer patients with elevated plasma fibrinogen levels and their attendant complications."}
{"id": "PMID:476621", "title": "Galactosyl transferase activity in human transitional cell carcinoma lines and in benign and neoplastic human bladder epithelium.", "content": "Cultured cells of human transitional cell carcinoma line MGH-U1, in suspension, were assayed for galactosyl transferase by measurement of the transfer of [3H]galactose from uridine diphosphate:[3H]galactose to desialylated ovine submaxillary mucin. The assay was optimized with respect to time and to protein, uridine disphosphate:galactose, desialyated ovine submaxillary mucin, and Triton X-100 concentrations. This assay was then applied to fresh specimens of benign, inflamed, and neoplastic bladder epithelium from 33 patients who under went cold-cup biopsies at cytoscopy. Transitional cell carcinoma specimens gave values in the range of 24.7 to 184.8 cpm [3H]galactose transferred per microgram protein per hr [72.0 +/- 44.7 (S.D.); n = 25]; normal and inflamed specimens ranged from 0.8 to 46.1 cpm/microgram protein per hr [8.3 +/- 8.4 (S.D.); n = 35]. By using a known method of cell rupture, cell ghosts, representing cell-surface membranes, were isolated both from the cultured cell line and from two biopsy specimens of transitional cell carcinoma. Although a complete enzymatic and electron microscopic analysis was not undertaken, the coincidence of an enzyme marker with the cell ghost fraction containing the elevated galactosyl transferase made it appear probable that this enzyme is located in the cell surface.", "contents": "Galactosyl transferase activity in human transitional cell carcinoma lines and in benign and neoplastic human bladder epithelium. Cultured cells of human transitional cell carcinoma line MGH-U1, in suspension, were assayed for galactosyl transferase by measurement of the transfer of [3H]galactose from uridine diphosphate:[3H]galactose to desialylated ovine submaxillary mucin. The assay was optimized with respect to time and to protein, uridine disphosphate:galactose, desialyated ovine submaxillary mucin, and Triton X-100 concentrations. This assay was then applied to fresh specimens of benign, inflamed, and neoplastic bladder epithelium from 33 patients who under went cold-cup biopsies at cytoscopy. Transitional cell carcinoma specimens gave values in the range of 24.7 to 184.8 cpm [3H]galactose transferred per microgram protein per hr [72.0 +/- 44.7 (S.D.); n = 25]; normal and inflamed specimens ranged from 0.8 to 46.1 cpm/microgram protein per hr [8.3 +/- 8.4 (S.D.); n = 35]. By using a known method of cell rupture, cell ghosts, representing cell-surface membranes, were isolated both from the cultured cell line and from two biopsy specimens of transitional cell carcinoma. Although a complete enzymatic and electron microscopic analysis was not undertaken, the coincidence of an enzyme marker with the cell ghost fraction containing the elevated galactosyl transferase made it appear probable that this enzyme is located in the cell surface."}
{"id": "PMID:476622", "title": "Effect of surgical removal on the growth and kinetics of residual tumor.", "content": "Findings from this study using a transplantable C3H mammary tumor failed to indicate interaction relative to growth parameters between two foci present in the same host. Whether they were growing alone or in the presence of a second focus, tumor growth rates were similar until the combined mass of multiple tumors approached that which was incompatible with survival. Only then was a difference in growth observed. Cytokinetic parameters, i.e., labeling index, primer-dependent DNA polymerase index or growth fraction, DNA synthesis time, tumor doubling time, and cell cycle time, were also similar whether tumors grew alone or in the presence of a second focus. Following removal of a tumor, changes were observed within 24 hr in the kinetics of the residual focus. There was an increase in labeling index (duration approximately equal to 10 days) and primer-dependent DNA polymerase index with a decrease in the tumor doubling time. Minimal change was noted in DNA synthesis time and cell cycle time. The kinetic changes observed were reflected in a measureable increase in tumor size approximately equal to a week following tumor removal. Absence of an alteration in DNA synthesis time and cell cycle time indicates that the increase in tumor growth was probably due to a conversion of noncycling cells in G0 phase into proliferation. Relationship of the findings to the use of adjuvant chemotherapy is considered.", "contents": "Effect of surgical removal on the growth and kinetics of residual tumor. Findings from this study using a transplantable C3H mammary tumor failed to indicate interaction relative to growth parameters between two foci present in the same host. Whether they were growing alone or in the presence of a second focus, tumor growth rates were similar until the combined mass of multiple tumors approached that which was incompatible with survival. Only then was a difference in growth observed. Cytokinetic parameters, i.e., labeling index, primer-dependent DNA polymerase index or growth fraction, DNA synthesis time, tumor doubling time, and cell cycle time, were also similar whether tumors grew alone or in the presence of a second focus. Following removal of a tumor, changes were observed within 24 hr in the kinetics of the residual focus. There was an increase in labeling index (duration approximately equal to 10 days) and primer-dependent DNA polymerase index with a decrease in the tumor doubling time. Minimal change was noted in DNA synthesis time and cell cycle time. The kinetic changes observed were reflected in a measureable increase in tumor size approximately equal to a week following tumor removal. Absence of an alteration in DNA synthesis time and cell cycle time indicates that the increase in tumor growth was probably due to a conversion of noncycling cells in G0 phase into proliferation. Relationship of the findings to the use of adjuvant chemotherapy is considered."}
{"id": "PMID:476623", "title": "Activity of thymidine as a chemotherapeutic agent against human tumor xenografts in nude mice.", "content": "The chemotherapeutic activity of thymidine (dThd) was tested against four human tumor xenografts growing in nude mice, including a melanoma, an oat cell carcinoma of the lung, a colon carcinoma, and a breast carcinoma. Tumor-bearing mice were given an infusion of dThd (1 g/kg/day) s.c. for 72 hr each week for three weeks. Tumor growth in the treated mice was compared to that in randomized concurrent control mice infused with media alone. A significant effect was found only for the melanoma, and it was cytostatic rather than cytotoxic. Even when melanomas of very small initial volume were treated, there were no complete regressions, and tumor growth resumed when dThd treatment was stopped. In culture, sustained dThd concentrations of greater than 3.2 mM were required to cause death of the melanoma cells; in the mice the dThd level during infusion ranged from 1 to 5 mM. This exposure to dThd, although failing to produce a tumor response, did produce significant toxicity in the nude mice in the form of myelosuppression and leukopenia. Flow cytometric analysis of marrow cells during the dThd infusion showed an accumulation of cells in S phase, but proliferation was not completely halted since cells with G2-M content of DNA were present in the marrow even after 72 hr of dThd exposure. This study failed to demonstrate a therapeutically useful effect of dThd on these tumors.", "contents": "Activity of thymidine as a chemotherapeutic agent against human tumor xenografts in nude mice. The chemotherapeutic activity of thymidine (dThd) was tested against four human tumor xenografts growing in nude mice, including a melanoma, an oat cell carcinoma of the lung, a colon carcinoma, and a breast carcinoma. Tumor-bearing mice were given an infusion of dThd (1 g/kg/day) s.c. for 72 hr each week for three weeks. Tumor growth in the treated mice was compared to that in randomized concurrent control mice infused with media alone. A significant effect was found only for the melanoma, and it was cytostatic rather than cytotoxic. Even when melanomas of very small initial volume were treated, there were no complete regressions, and tumor growth resumed when dThd treatment was stopped. In culture, sustained dThd concentrations of greater than 3.2 mM were required to cause death of the melanoma cells; in the mice the dThd level during infusion ranged from 1 to 5 mM. This exposure to dThd, although failing to produce a tumor response, did produce significant toxicity in the nude mice in the form of myelosuppression and leukopenia. Flow cytometric analysis of marrow cells during the dThd infusion showed an accumulation of cells in S phase, but proliferation was not completely halted since cells with G2-M content of DNA were present in the marrow even after 72 hr of dThd exposure. This study failed to demonstrate a therapeutically useful effect of dThd on these tumors."}
{"id": "PMID:476624", "title": "Phase I clinical and pharmacological study of 4'-(9-acridinylamino)-methanesulfon-m-anisidide using an intermittent biweekly schedule.", "content": "4'-(9-Acridinylamino)methanesulfon-m-anisidide (m-AMSA, NSC 249992), an acridine derivative, was given to 28 patients with solid tumors and one patient with Hodgkin's disease in a Phase I clinical trial. The dose schedule used was a single dose given every 14 days for three doses. The amount given ranged from 10 to 120 mg/sq m/dose. Dose-limiting toxicity was moderate to severe leukopenia which occurred at and above 70 mg/sq m. Thrombocytopenia was infrequent and did not require transfusion. Nonhematological side effects were mild and included nausea, vomiting, local irritation, and fever. Antineoplastic activity was noted in liposarcoma, adenocarcinoma of unknown primary origin, and squamous carcinoma of unknown primary origin (one patient each). Pharmacokinetics studies were done in 19 patients. Total m-AMSA and free m-AMSA concentrations showed a biphasic distribution with an initial rapid phase of t1/2 = 10 to 15 min for both, and a second slow phase of t1/2 = 8 to 9 hr for total m-AMSA and 3 hr for free m-AMSA. Phase II studies with m-AMSA, in hematological cancers are warranted, since its most consistent effect is on leukocytes. The recommended dosages for solid-tumor Phase II studies are 70 mg/sq m for good-risk patients and 50 mg/sq m for poor-risk patients, given as a single dose every other week, or 120 mg/sq m for poor-risk patients for the single-dose every-3-week schedule.", "contents": "Phase I clinical and pharmacological study of 4'-(9-acridinylamino)-methanesulfon-m-anisidide using an intermittent biweekly schedule. 4'-(9-Acridinylamino)methanesulfon-m-anisidide (m-AMSA, NSC 249992), an acridine derivative, was given to 28 patients with solid tumors and one patient with Hodgkin's disease in a Phase I clinical trial. The dose schedule used was a single dose given every 14 days for three doses. The amount given ranged from 10 to 120 mg/sq m/dose. Dose-limiting toxicity was moderate to severe leukopenia which occurred at and above 70 mg/sq m. Thrombocytopenia was infrequent and did not require transfusion. Nonhematological side effects were mild and included nausea, vomiting, local irritation, and fever. Antineoplastic activity was noted in liposarcoma, adenocarcinoma of unknown primary origin, and squamous carcinoma of unknown primary origin (one patient each). Pharmacokinetics studies were done in 19 patients. Total m-AMSA and free m-AMSA concentrations showed a biphasic distribution with an initial rapid phase of t1/2 = 10 to 15 min for both, and a second slow phase of t1/2 = 8 to 9 hr for total m-AMSA and 3 hr for free m-AMSA. Phase II studies with m-AMSA, in hematological cancers are warranted, since its most consistent effect is on leukocytes. The recommended dosages for solid-tumor Phase II studies are 70 mg/sq m for good-risk patients and 50 mg/sq m for poor-risk patients, given as a single dose every other week, or 120 mg/sq m for poor-risk patients for the single-dose every-3-week schedule."}
{"id": "PMID:476626", "title": "Establishment of photoaffinity label derivatives of fluorene as probes in studies of chemical carcinogenesis in mammalian cell culture.", "content": "Several azido fluorenes, photosensitive analogs of the established carcinogen 2-acetylaminofluorene, have been synthesized to serve as probes in chemical carcinogenesis and mutagenesis studies. Unlike 2-acetylaminofluorene, these compounds do not require metabolic activation. However, alkylation to critical targets is achieved through the generation of nitrenes by photolysis at 360 nm in situ. We have found that the bifunctional azidofluorenes 2,5-diazidofluorene and 2,7-diazidofluorene were more toxic to and more transforming of the mouse embryo C3H 10T1/2 clone 8 cells when photolyzed in situ than were the monofunctional azides 2-azidofluorene and 7-bromo-2-azidofluorene. When the drugs were photolyzed in phosphate-buffered saline (pH 7.4) and then added to the cells these preirradiated derivatives were not transforming but were slightly toxic. However, there is not relationship between the number of photosensitive azido groups and the cytotoxicities of the preirradiated derivatives as measured by the plating efficiency method. The transformation experiments were performed under conditions in which photolysis was carried out for as little as 15 sec. This is significant because the major events that lead to oncogenesis apparently can occur within this narrow time channel, thereby making the drugs excellent probes in studies in chemical carcinogenesis. Although near ultraviolet light alone may transform the cells (8 to 16 min of irradiation), under the conditions studied ultraviolet light was neither toxic nor transforming.", "contents": "Establishment of photoaffinity label derivatives of fluorene as probes in studies of chemical carcinogenesis in mammalian cell culture. Several azido fluorenes, photosensitive analogs of the established carcinogen 2-acetylaminofluorene, have been synthesized to serve as probes in chemical carcinogenesis and mutagenesis studies. Unlike 2-acetylaminofluorene, these compounds do not require metabolic activation. However, alkylation to critical targets is achieved through the generation of nitrenes by photolysis at 360 nm in situ. We have found that the bifunctional azidofluorenes 2,5-diazidofluorene and 2,7-diazidofluorene were more toxic to and more transforming of the mouse embryo C3H 10T1/2 clone 8 cells when photolyzed in situ than were the monofunctional azides 2-azidofluorene and 7-bromo-2-azidofluorene. When the drugs were photolyzed in phosphate-buffered saline (pH 7.4) and then added to the cells these preirradiated derivatives were not transforming but were slightly toxic. However, there is not relationship between the number of photosensitive azido groups and the cytotoxicities of the preirradiated derivatives as measured by the plating efficiency method. The transformation experiments were performed under conditions in which photolysis was carried out for as little as 15 sec. This is significant because the major events that lead to oncogenesis apparently can occur within this narrow time channel, thereby making the drugs excellent probes in studies in chemical carcinogenesis. Although near ultraviolet light alone may transform the cells (8 to 16 min of irradiation), under the conditions studied ultraviolet light was neither toxic nor transforming."}
{"id": "PMID:476629", "title": "Isolation and characterization of canine venereal tumor-associated inhibitory and blocking factors.", "content": "Spontaneous regression of the canine venereal tumor is associated with the production of a serum factor which inhibits in vitro tumor colony-forming units in agar. Logarithmic or persistent tumor growth, on the other hand, is characterized by a serum factor which protects cells against in vitro inhibition (blocking factor). These factors have been characterized by immunochemical methods. Whole regressor and blocking sera were fractionated by Sephadex G-200 filtration and immunoabsorption with rabbit antiserum specific for canine immunoglobulin G2a. Fractions were characterized by immunoelectrophoresis, radial immunodiffusion, and disc gel electrophoresis. In vitro inhibitory and blocking activity of the whole serum was accounted for by the purified immunoglobulin G2a. Blocking activity was also found in protein eluted from logarithmically growing tumors. Preparative polyacrylamide electrophoresis revealed five major fractions with blocking activity only in the immunoglobulin G fraction. Tumor eluates and immunoglobulin G isolated from serially removed tumors demonstrated with the clinical course of the tumor. Using ultrafiltration and sodium dodecyl sulfate electrophoresis of tumor-associated immunoglobulin G at low pH, it was not possible to identify an antigen complexed to the blocking antibody.", "contents": "Isolation and characterization of canine venereal tumor-associated inhibitory and blocking factors. Spontaneous regression of the canine venereal tumor is associated with the production of a serum factor which inhibits in vitro tumor colony-forming units in agar. Logarithmic or persistent tumor growth, on the other hand, is characterized by a serum factor which protects cells against in vitro inhibition (blocking factor). These factors have been characterized by immunochemical methods. Whole regressor and blocking sera were fractionated by Sephadex G-200 filtration and immunoabsorption with rabbit antiserum specific for canine immunoglobulin G2a. Fractions were characterized by immunoelectrophoresis, radial immunodiffusion, and disc gel electrophoresis. In vitro inhibitory and blocking activity of the whole serum was accounted for by the purified immunoglobulin G2a. Blocking activity was also found in protein eluted from logarithmically growing tumors. Preparative polyacrylamide electrophoresis revealed five major fractions with blocking activity only in the immunoglobulin G fraction. Tumor eluates and immunoglobulin G isolated from serially removed tumors demonstrated with the clinical course of the tumor. Using ultrafiltration and sodium dodecyl sulfate electrophoresis of tumor-associated immunoglobulin G at low pH, it was not possible to identify an antigen complexed to the blocking antibody."}
{"id": "PMID:476628", "title": "Effect of phorbol ester tumor promoters on the expression of melanogenesis in B-16 melanoma cells.", "content": "Cells of the C3 clone of B-16 melanoma synthesize melanin only at confluence after which they senesce and can no longer be passaged. Addition to the cultures of 10(-8)--10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA) shortly after plating delayed by about 2 days the onset of melanogenesis. TPA did not, however, affect the growth of the cells or the time at which they reached confluence. The ability of a series of phorbol esters to delay melanogenesis correlated with their tumor-promoting activity on mouse skin. The optimum time for addition of TPA was within the first 24 hr after plating; the inhibitory effect decreased when TPA was added at later points. alpha-melanocyte-stimulating hormone (5 x 10(-7) M) added to B-16 cultures 24 hr after plating slowed the growth of the cells and caused them to differentiate when still subconfluent. TPA also inhibited this alpha-melanocyte-stimulating hormone-induced melanogenesis. These results suggest that TPA inhibits a very early stage in a stepwise process that leads to the differentiation of these cultures. For reasons that are not apparent, the cells eventually escape from this inhibition. The B-16 melanoma cell culture system may be useful for studying the mechanism by which TPA and related tumor promoters affect cellular differentiation.", "contents": "Effect of phorbol ester tumor promoters on the expression of melanogenesis in B-16 melanoma cells. Cells of the C3 clone of B-16 melanoma synthesize melanin only at confluence after which they senesce and can no longer be passaged. Addition to the cultures of 10(-8)--10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA) shortly after plating delayed by about 2 days the onset of melanogenesis. TPA did not, however, affect the growth of the cells or the time at which they reached confluence. The ability of a series of phorbol esters to delay melanogenesis correlated with their tumor-promoting activity on mouse skin. The optimum time for addition of TPA was within the first 24 hr after plating; the inhibitory effect decreased when TPA was added at later points. alpha-melanocyte-stimulating hormone (5 x 10(-7) M) added to B-16 cultures 24 hr after plating slowed the growth of the cells and caused them to differentiate when still subconfluent. TPA also inhibited this alpha-melanocyte-stimulating hormone-induced melanogenesis. These results suggest that TPA inhibits a very early stage in a stepwise process that leads to the differentiation of these cultures. For reasons that are not apparent, the cells eventually escape from this inhibition. The B-16 melanoma cell culture system may be useful for studying the mechanism by which TPA and related tumor promoters affect cellular differentiation."}
{"id": "PMID:476631", "title": "Formation of carbonyl chloride in carbon tetrachloride metabolism by rat liver in vitro.", "content": "In order to identify intermediates of CCl4 metabolism, whole, suitably fortified rat liver homogenates were incubated with 14CCl4 in the presence and absence of \"pools\" of unlabeled suspected intermediates. In the presence of NADH or NADPH, incorporation of radioactivity was rapid and substantial in CO2, lipid, protein, and the acid-soluble fraction. It was not influenced by the presence of large pools of unlabeled chloroform or formate, thus excluding these substances as obligatory intermediates. However, when incubated with L-cysteine, radioactivity incorporation in the acid-soluble fraction was almost doubled, and about one-third of the radioactivity of this fraction was identified as 2-oxothiazolidine 4-carboxylic acid. This substance is formed chemically by condensation of cysteine with carbonyl chloride and has been identified previously by others as a product of chloroform metabolism by liver microsomes in the presence of L-cysteine. Based on current knowledge of CCl4 metabolism, the following aerobic pathway is envisioned: microsomal cleavage to Cl- and .CCl3 and oxidation of the latter to the unstable intermediate, Cl3COH, which loses HCl to yield COCl2. COCl2 is likely to be the major source of CO2 from CCl4 but is probably not the intermediate that binds to lipid and protein. The addition of glutathione had no effect on CCl4 metabolism in rat liver homogenate, suggesting that glutathione S-transferases, which catalyze other dehalogenation reactions, do not play a role in CCl4 metabolism.", "contents": "Formation of carbonyl chloride in carbon tetrachloride metabolism by rat liver in vitro. In order to identify intermediates of CCl4 metabolism, whole, suitably fortified rat liver homogenates were incubated with 14CCl4 in the presence and absence of \"pools\" of unlabeled suspected intermediates. In the presence of NADH or NADPH, incorporation of radioactivity was rapid and substantial in CO2, lipid, protein, and the acid-soluble fraction. It was not influenced by the presence of large pools of unlabeled chloroform or formate, thus excluding these substances as obligatory intermediates. However, when incubated with L-cysteine, radioactivity incorporation in the acid-soluble fraction was almost doubled, and about one-third of the radioactivity of this fraction was identified as 2-oxothiazolidine 4-carboxylic acid. This substance is formed chemically by condensation of cysteine with carbonyl chloride and has been identified previously by others as a product of chloroform metabolism by liver microsomes in the presence of L-cysteine. Based on current knowledge of CCl4 metabolism, the following aerobic pathway is envisioned: microsomal cleavage to Cl- and .CCl3 and oxidation of the latter to the unstable intermediate, Cl3COH, which loses HCl to yield COCl2. COCl2 is likely to be the major source of CO2 from CCl4 but is probably not the intermediate that binds to lipid and protein. The addition of glutathione had no effect on CCl4 metabolism in rat liver homogenate, suggesting that glutathione S-transferases, which catalyze other dehalogenation reactions, do not play a role in CCl4 metabolism."}
{"id": "PMID:476632", "title": "Utilization of 2'-deoxy-6-thioguanosine 5'-triphosphate in DNA synthesis in vitro by DNA polymerase alpha from calf thymus.", "content": "Chemically synthesized beta-2'-deoxy-6-thioguanosine 5'-triphosphate, a putative active form of beta-2'-deoxy-6-thioguanosine, was used efficiently as a substrate for DNA synthesis catalyzed by DNA polymerase alpha from calf thymus. The deoxythioguanylate was incorporated into DNA by replacing deoxyguanylate and supported the further elongation of DNA chains on activated calf thymus DNA. In contrast, DNA polymerase beta used beta-2'-deoxy-6-thioguanosine 5'-triphosphate at a much lower rate. The reaction product of DNA polymerase alpha, i.e., 6-thioguanine-containing DNA, adsorbed specifically to the organomercurial agarose column, and showed a peak of UV absorption at 342 nm, which is characteristic of thioguanine.", "contents": "Utilization of 2'-deoxy-6-thioguanosine 5'-triphosphate in DNA synthesis in vitro by DNA polymerase alpha from calf thymus. Chemically synthesized beta-2'-deoxy-6-thioguanosine 5'-triphosphate, a putative active form of beta-2'-deoxy-6-thioguanosine, was used efficiently as a substrate for DNA synthesis catalyzed by DNA polymerase alpha from calf thymus. The deoxythioguanylate was incorporated into DNA by replacing deoxyguanylate and supported the further elongation of DNA chains on activated calf thymus DNA. In contrast, DNA polymerase beta used beta-2'-deoxy-6-thioguanosine 5'-triphosphate at a much lower rate. The reaction product of DNA polymerase alpha, i.e., 6-thioguanine-containing DNA, adsorbed specifically to the organomercurial agarose column, and showed a peak of UV absorption at 342 nm, which is characteristic of thioguanine."}
{"id": "PMID:476633", "title": "Influence of factors derived from EMT6 tumors and from bone marrow of tumor-bearing mice on tumor and bone marrow stem cell kinetics.", "content": "Untreated EMT6 tumors in BALB/c mice were used to assess the regulatory mechanisms of tumor growth in these animals. This tumor can be quantitated for clonogenic cells by in vitro techniques, and the hydroxyurea suicide method makes it possible to determine the kinetic status of the clonogenic cells. The untreated EMT6 tumor does not seem to have internal humoral regulatory mechanisms explaining tumor growth kinetics. However, the exponentially growing EMT6 experimental tumor releases a factor capable of stimulating quiescent splenic colony-forming units into cycle. This is also true of bone marrow taken from tumor-bearing mice. This study was made possible using an in vivo-in vitro technique which separates the effector cells from the responder cells by a Millipore filter floating on the culture medium. The relationship between tumor growth and normal hematopoietic tissue of the tumor-bearing animal is discussed.", "contents": "Influence of factors derived from EMT6 tumors and from bone marrow of tumor-bearing mice on tumor and bone marrow stem cell kinetics. Untreated EMT6 tumors in BALB/c mice were used to assess the regulatory mechanisms of tumor growth in these animals. This tumor can be quantitated for clonogenic cells by in vitro techniques, and the hydroxyurea suicide method makes it possible to determine the kinetic status of the clonogenic cells. The untreated EMT6 tumor does not seem to have internal humoral regulatory mechanisms explaining tumor growth kinetics. However, the exponentially growing EMT6 experimental tumor releases a factor capable of stimulating quiescent splenic colony-forming units into cycle. This is also true of bone marrow taken from tumor-bearing mice. This study was made possible using an in vivo-in vitro technique which separates the effector cells from the responder cells by a Millipore filter floating on the culture medium. The relationship between tumor growth and normal hematopoietic tissue of the tumor-bearing animal is discussed."}
{"id": "PMID:476634", "title": "Sensitivity to chemotherapeutic and immunomodulating agents of two mouse lymphomas and of a macrophage tumor.", "content": "The effect of treatment with 15 chemotherapeutic and 10 immunomodulating agents on the growth of T-cell lymphoma EL-4, macrophage tumor J774, and B-cell lymphoma 70Z/2 of the mouse has been studied using the prolongation of median survival time of tumor-bearing hosts as an index of therapeutic effectiveness. The survival time of mice bearing 70Z/2 was prolonged more than 100% by single-agent therapy with actinomycin D, cyclophosphamide, 6-mercaptopurine, mitomycin C, and vinblastine; a similar response of J774 was produced by therapy with Adriamycin, cyclophosphamide, or 6-mercaptopurine. No chemotherapeutic agent prolonged the median survival time of mice bearing EL-4 by 100% or more. Of the immunomodulating agents, mycobacterial preparations (Bacillus Calmette-Gu\u00e9rin or interphase material), Corynebacterium parvum, and polyinosinic-polycytidylic acid moderately prolonged the survival time of mice bearing J774 or 70Z/2; the EL-4 lymphoma was refractory to all 10 immunomodulating agents.", "contents": "Sensitivity to chemotherapeutic and immunomodulating agents of two mouse lymphomas and of a macrophage tumor. The effect of treatment with 15 chemotherapeutic and 10 immunomodulating agents on the growth of T-cell lymphoma EL-4, macrophage tumor J774, and B-cell lymphoma 70Z/2 of the mouse has been studied using the prolongation of median survival time of tumor-bearing hosts as an index of therapeutic effectiveness. The survival time of mice bearing 70Z/2 was prolonged more than 100% by single-agent therapy with actinomycin D, cyclophosphamide, 6-mercaptopurine, mitomycin C, and vinblastine; a similar response of J774 was produced by therapy with Adriamycin, cyclophosphamide, or 6-mercaptopurine. No chemotherapeutic agent prolonged the median survival time of mice bearing EL-4 by 100% or more. Of the immunomodulating agents, mycobacterial preparations (Bacillus Calmette-Gu\u00e9rin or interphase material), Corynebacterium parvum, and polyinosinic-polycytidylic acid moderately prolonged the survival time of mice bearing J774 or 70Z/2; the EL-4 lymphoma was refractory to all 10 immunomodulating agents."}
{"id": "PMID:476636", "title": "Effect of the duration of retinyl acetate feeding on inhibition of 1-methyl-1-nitrosourea-induced mammary carcinogenesis in the rat.", "content": "The effect of the duration of retinoid treatment on the inhibition of 1-methyl-1-nitrosourea-induced mammary carcinogenesis was studied. Female Sprague-Dawley rats were given i.v. injections of 50 mg 1-methyl-1-nitrosourea per kg body weight at both 50 and 57 days of age. Feeding of a placebo diet or diet supplemented with 323 mg retinyl acetate per kg diet (retinoid treatment) was initiated at 10 days after the first carcinogen injection. Retinoid treatment was either continued or discontinued after 60 days postcarcinogen, and the study was terminated at 182 days postcarcinogen. Retinoid treatment between 10 and 60 days postcarcinogen prolonged the cancer latency and reduced the average number of cancers per rat in comparison to that in placebo-treated rats. Continuation or cessation of retinoid treatment in 60-day tumor-bearing rats had no effect on the time of appearance of additional cancers. In 60-day tumor-free rats, continuation of retinoid treatment prolonged cancer latency in comparison to either 60-day tumor-free rats changed to placebo or rats continuously treated with placebo. The cessation of retinoid treatment in 60-day tumor-free rats resulted in a rapid increase in the appearance of cancers; at the termination of the study, the average number of cancers per rat was similar to that of animals fed only the placebo. The data indicated that some rats are more responsive to the retinoid than are others. Retinoid treatment apparently prevented the progression of early neoplastic lesions, and a continuous daily intake of the retinoid appears necessary to sustain the chemopreventive effect under the experimental conditions imposed.", "contents": "Effect of the duration of retinyl acetate feeding on inhibition of 1-methyl-1-nitrosourea-induced mammary carcinogenesis in the rat. The effect of the duration of retinoid treatment on the inhibition of 1-methyl-1-nitrosourea-induced mammary carcinogenesis was studied. Female Sprague-Dawley rats were given i.v. injections of 50 mg 1-methyl-1-nitrosourea per kg body weight at both 50 and 57 days of age. Feeding of a placebo diet or diet supplemented with 323 mg retinyl acetate per kg diet (retinoid treatment) was initiated at 10 days after the first carcinogen injection. Retinoid treatment was either continued or discontinued after 60 days postcarcinogen, and the study was terminated at 182 days postcarcinogen. Retinoid treatment between 10 and 60 days postcarcinogen prolonged the cancer latency and reduced the average number of cancers per rat in comparison to that in placebo-treated rats. Continuation or cessation of retinoid treatment in 60-day tumor-bearing rats had no effect on the time of appearance of additional cancers. In 60-day tumor-free rats, continuation of retinoid treatment prolonged cancer latency in comparison to either 60-day tumor-free rats changed to placebo or rats continuously treated with placebo. The cessation of retinoid treatment in 60-day tumor-free rats resulted in a rapid increase in the appearance of cancers; at the termination of the study, the average number of cancers per rat was similar to that of animals fed only the placebo. The data indicated that some rats are more responsive to the retinoid than are others. Retinoid treatment apparently prevented the progression of early neoplastic lesions, and a continuous daily intake of the retinoid appears necessary to sustain the chemopreventive effect under the experimental conditions imposed."}
{"id": "PMID:476635", "title": "Hormonally induced tumors of the reproductive system of parabiosed male rats.", "content": "Parabiosis of intact male rats to castrated males or oophorectomized females for a period of approximately 20 months resulted in three interstitial cell tumors of the testis. When unilateral nephrectomy was added to the parabiotic procedure in ten pairs, eight interstitial cell tumors of the testis and four adenocarcinomas of the prostate occurred in the target male parabionts. These changes were preceded by elevations in luteinizing and follicle-stimulating hormone levels in the serum of the castrates and high levels of testosterone and, to a lesser degree, of androstenedion in the target partners developing the tumors.", "contents": "Hormonally induced tumors of the reproductive system of parabiosed male rats. Parabiosis of intact male rats to castrated males or oophorectomized females for a period of approximately 20 months resulted in three interstitial cell tumors of the testis. When unilateral nephrectomy was added to the parabiotic procedure in ten pairs, eight interstitial cell tumors of the testis and four adenocarcinomas of the prostate occurred in the target male parabionts. These changes were preceded by elevations in luteinizing and follicle-stimulating hormone levels in the serum of the castrates and high levels of testosterone and, to a lesser degree, of androstenedion in the target partners developing the tumors."}
{"id": "PMID:476637", "title": "Effect of tetrahydrouridine on the action of 1-beta-D-arabinofuranosylcytosine in synchronized cultures of normal rat kidney cells.", "content": "Tetrahydrouridine (THU) is a reduced pyrimidine nucleoside and has been found to be a potent cytidine deaminase inhibitor. We have examined the effect of THU on the action of 1-beta-D-arabinofuranosylcytosine (ara-C) on DNA synthesis and its cytotoxic effect on normal rat kidney (NRK) cells. The results indicated that THU, when present alone, has no effect on the DNA synthesis or viability of the NRK cells. The addition of THU can, however, further increase the cytotoxic effect of sublethal doses of ara-C and can enhance its ability to inhibit DNA synthesis. Studies on the duration of exposure of the NRK cells to ara-C and on the effect of ara-C on synchronized cultures of NRK cells also support the earlier reports that ara C, through its active compound 1-beta-D-arabinofuranosylcytosine 5'-triphosphate, is capable of blocking cells from progression from G1 into S phase of the cell cycle. Furthermore, we have shown that this inhibition is reversible after the drug has been removed. The addition of THU in the presence of noninhibiting levels of ara-C can also result in the blocking of cells from progression into S phase of the cell cycle. Similar to the effect of ara-C, this inhibition is also reversible. These studies suggest that the addition of THU enhances the cytotoxicity of sublethal doses of ara-C and its ability to inhibit DNA synthesis.", "contents": "Effect of tetrahydrouridine on the action of 1-beta-D-arabinofuranosylcytosine in synchronized cultures of normal rat kidney cells. Tetrahydrouridine (THU) is a reduced pyrimidine nucleoside and has been found to be a potent cytidine deaminase inhibitor. We have examined the effect of THU on the action of 1-beta-D-arabinofuranosylcytosine (ara-C) on DNA synthesis and its cytotoxic effect on normal rat kidney (NRK) cells. The results indicated that THU, when present alone, has no effect on the DNA synthesis or viability of the NRK cells. The addition of THU can, however, further increase the cytotoxic effect of sublethal doses of ara-C and can enhance its ability to inhibit DNA synthesis. Studies on the duration of exposure of the NRK cells to ara-C and on the effect of ara-C on synchronized cultures of NRK cells also support the earlier reports that ara C, through its active compound 1-beta-D-arabinofuranosylcytosine 5'-triphosphate, is capable of blocking cells from progression from G1 into S phase of the cell cycle. Furthermore, we have shown that this inhibition is reversible after the drug has been removed. The addition of THU in the presence of noninhibiting levels of ara-C can also result in the blocking of cells from progression into S phase of the cell cycle. Similar to the effect of ara-C, this inhibition is also reversible. These studies suggest that the addition of THU enhances the cytotoxicity of sublethal doses of ara-C and its ability to inhibit DNA synthesis."}
{"id": "PMID:476639", "title": "Physicochemical differences between glucocorticoid-binding components from the corticoid-sensitive and -resistant strains of mouse lymphoma P1798.", "content": "The physicochemical properties of nuclear and cytosolic glucocorticoid-binding components from corticoid-sensitive (CS) and corticoid-resistant (CR) mouse lymphoma P1798 cells have been compared. Nuclei or cytosol fractions were prepared from lymphocytes that had been labeled at 37 or 4 degrees, respectively, with 30 nM [3H]triamcinolone acetonide ([3H]TA). [3H]TA was extracted with 0.6 M KCl, 10 mM spermidine, or 4.5 mM MgCl2 from CS nuclei and with 0.6 M KCl or 10 mM spermidine from CR nuclei. As reported previously, nuclear-associated [3H]TA in CR cells was resistant to extraction with mM concentrations of MgCl2. Loss of bound steroid during extraction with 0.6 M KCl was minimized by including the chymotrypsin inhibitor, carbobenzoxy-L-phenylalanine, in the extraction buffer. The inhibitor was not required during extraction with spermidine or MgCl2. Nuclear and cytosolic extracts were examined by analytical agarose gel filtration and glycerol density gradient centrifugation under high salt (0.6 M KCl) conditions. The glucocorticoid-binding component in KCl, spermidine, and MgCl2 extracts from CS nuclei was considerably larger and more asymmetrical [Stokes radius, 57 to 59 A; sedimentation coefficient, 3.64 to 3.70S; molecular weight, 90,000 daltons; frictional ratio, 1.8; axial ratio (prolate ellipsoid), 15] than the [3H]TA-macromolecular complex in KCl and spermidine extracts from CR nuclei[Stokes radius, 29 A; sedimentation coefficient, 3.23 to 3.30S; molecular weight, 40,000 daltons; frictional ratio, 1.25; axial ratio (prolate ellipsoid), 5]. Control experiments showed that the smaller size of the glucocorticoid-binding component in CR nuclei was probably not due to cleavage of a larger, CS-like complex during the extraction procedure. The larger size of the CS [3H]TA complex did not appear to result from aggregation of s a smaller species. No difference in physicochemical parameters of the binding component was observed if cells were labeled with [3H]dexamethasone instead of [3H]TA. However, [3H]dexamethasone complexes were less stable than those formed with [3H]TA as indicated by considerable dissociation of [3H]dexamethasone during gel filtration and gradient centrifugation. This may be due to the 3- to 5-fold lower relative binding affinity of [3H]dexamethasone. Analysis of [3H]TA-labeled cytosol by gel filtration and gradient centrifugation revealed the presence of a single binding component with physicochemical properties similar to those of nuclear [3H]TA complexes from the same strain of tumor. These results suggest that previously described differences in extractability of nuclear-associated [3H]TA between the CS and CR strains of mouse lymphoma P1798 and the lack of response of CR P1798 to glucocorticoid administration may be due, at least in part, to the presence of an altered glucocorticoid-binding component in the resistant tumor cells.", "contents": "Physicochemical differences between glucocorticoid-binding components from the corticoid-sensitive and -resistant strains of mouse lymphoma P1798. The physicochemical properties of nuclear and cytosolic glucocorticoid-binding components from corticoid-sensitive (CS) and corticoid-resistant (CR) mouse lymphoma P1798 cells have been compared. Nuclei or cytosol fractions were prepared from lymphocytes that had been labeled at 37 or 4 degrees, respectively, with 30 nM [3H]triamcinolone acetonide ([3H]TA). [3H]TA was extracted with 0.6 M KCl, 10 mM spermidine, or 4.5 mM MgCl2 from CS nuclei and with 0.6 M KCl or 10 mM spermidine from CR nuclei. As reported previously, nuclear-associated [3H]TA in CR cells was resistant to extraction with mM concentrations of MgCl2. Loss of bound steroid during extraction with 0.6 M KCl was minimized by including the chymotrypsin inhibitor, carbobenzoxy-L-phenylalanine, in the extraction buffer. The inhibitor was not required during extraction with spermidine or MgCl2. Nuclear and cytosolic extracts were examined by analytical agarose gel filtration and glycerol density gradient centrifugation under high salt (0.6 M KCl) conditions. The glucocorticoid-binding component in KCl, spermidine, and MgCl2 extracts from CS nuclei was considerably larger and more asymmetrical [Stokes radius, 57 to 59 A; sedimentation coefficient, 3.64 to 3.70S; molecular weight, 90,000 daltons; frictional ratio, 1.8; axial ratio (prolate ellipsoid), 15] than the [3H]TA-macromolecular complex in KCl and spermidine extracts from CR nuclei[Stokes radius, 29 A; sedimentation coefficient, 3.23 to 3.30S; molecular weight, 40,000 daltons; frictional ratio, 1.25; axial ratio (prolate ellipsoid), 5]. Control experiments showed that the smaller size of the glucocorticoid-binding component in CR nuclei was probably not due to cleavage of a larger, CS-like complex during the extraction procedure. The larger size of the CS [3H]TA complex did not appear to result from aggregation of s a smaller species. No difference in physicochemical parameters of the binding component was observed if cells were labeled with [3H]dexamethasone instead of [3H]TA. However, [3H]dexamethasone complexes were less stable than those formed with [3H]TA as indicated by considerable dissociation of [3H]dexamethasone during gel filtration and gradient centrifugation. This may be due to the 3- to 5-fold lower relative binding affinity of [3H]dexamethasone. Analysis of [3H]TA-labeled cytosol by gel filtration and gradient centrifugation revealed the presence of a single binding component with physicochemical properties similar to those of nuclear [3H]TA complexes from the same strain of tumor. These results suggest that previously described differences in extractability of nuclear-associated [3H]TA between the CS and CR strains of mouse lymphoma P1798 and the lack of response of CR P1798 to glucocorticoid administration may be due, at least in part, to the presence of an altered glucocorticoid-binding component in the resistant tumor cells."}
{"id": "PMID:476640", "title": "Immune-mediated arrest and reversal of established visceral metastases in athymic mice.", "content": "The metastasizing MDAY-D2 tumor of DBA/2 mice disseminates in BALB/c allogeneic athymic nude (nu/nu) mice in a manner identical to that observed in the syngeneic host. Both the kinetics and organ distribution pattern of metastases from s.c. implants of MDAY-D2 are routinely predictable at any given tumor dose. BALB/c heterozygote (nu/+) litter-mates reject MDAY-D2 grafts on the basis of the multiple minor histocompatibility differences that exist between DBA/2 and BALB/c mice. The in vitro cell-mediated cytotoxic response detected in tumor-bearing BALB/c nu/+ mice is \"low grade\" (isotope release is approximately 40 to 50% by 24-hr 111-indium-8-hydroxyquinoline assay and approximately 6 to 8% by 6-hr 51Cr assay) and yet correlates directly with tumor rejection. BALB/c nu/nu mice can be protected against MDAY-D2 by previous reconstitution with lymphoid cells from normal or MDAY-D2-sensitized BALB/c nu/+ mice. In addition, surgically documented, established visceral metastases in BALB/c nu/nu mice can be arrested and regressed by the adoptive transfer of MDAY-D2-sensitized BALB/c nu/+ spleen cells. This represents one of the few models where established metastases have been immunotherapeutically regressed. As such, the MDAY-D2 BALB/c nu/nu mouse model offers unique advantages for studying the role of the immune system in regulating the metastatic process.", "contents": "Immune-mediated arrest and reversal of established visceral metastases in athymic mice. The metastasizing MDAY-D2 tumor of DBA/2 mice disseminates in BALB/c allogeneic athymic nude (nu/nu) mice in a manner identical to that observed in the syngeneic host. Both the kinetics and organ distribution pattern of metastases from s.c. implants of MDAY-D2 are routinely predictable at any given tumor dose. BALB/c heterozygote (nu/+) litter-mates reject MDAY-D2 grafts on the basis of the multiple minor histocompatibility differences that exist between DBA/2 and BALB/c mice. The in vitro cell-mediated cytotoxic response detected in tumor-bearing BALB/c nu/+ mice is \"low grade\" (isotope release is approximately 40 to 50% by 24-hr 111-indium-8-hydroxyquinoline assay and approximately 6 to 8% by 6-hr 51Cr assay) and yet correlates directly with tumor rejection. BALB/c nu/nu mice can be protected against MDAY-D2 by previous reconstitution with lymphoid cells from normal or MDAY-D2-sensitized BALB/c nu/+ mice. In addition, surgically documented, established visceral metastases in BALB/c nu/nu mice can be arrested and regressed by the adoptive transfer of MDAY-D2-sensitized BALB/c nu/+ spleen cells. This represents one of the few models where established metastases have been immunotherapeutically regressed. As such, the MDAY-D2 BALB/c nu/nu mouse model offers unique advantages for studying the role of the immune system in regulating the metastatic process."}
{"id": "PMID:476641", "title": "Advanced stage and early relapse of breast carcinomas associated with high thymidine labeling indices.", "content": "The thymidine labeling index (TLI) showed a lognormal distribution for 133 primary breast carcinomas with a median of 2.21 and a range of 0.04 to 18.6/100. High TLI's were associated significantly with nuclear anaplasia, pushing tumor border, necrosis, cellular inflammation, and total replacement of lymph nodes involved by tumor, and the data suggested an association of high TLI with large primary tumor and the presence of axillary metastasis. Five patients who initially had local or distant spread all had above median TLI's. They and the 38 patients who developed recurrent tumor during a mean interval of 3.2 years of observation after mastectomy showed a higher geometric mean TLI than those who remained tumor-free (2.93 versus 1.85; p less than 0.05). The rate of early relapse for operable patients with above-median TLI's was significantly greater than that for patients with below-median TLI's (p = 0.0018). High TLI appears to be associated with rapid evolution of breast carcinoma both prior to and following diagnosis and treatment.", "contents": "Advanced stage and early relapse of breast carcinomas associated with high thymidine labeling indices. The thymidine labeling index (TLI) showed a lognormal distribution for 133 primary breast carcinomas with a median of 2.21 and a range of 0.04 to 18.6/100. High TLI's were associated significantly with nuclear anaplasia, pushing tumor border, necrosis, cellular inflammation, and total replacement of lymph nodes involved by tumor, and the data suggested an association of high TLI with large primary tumor and the presence of axillary metastasis. Five patients who initially had local or distant spread all had above median TLI's. They and the 38 patients who developed recurrent tumor during a mean interval of 3.2 years of observation after mastectomy showed a higher geometric mean TLI than those who remained tumor-free (2.93 versus 1.85; p less than 0.05). The rate of early relapse for operable patients with above-median TLI's was significantly greater than that for patients with below-median TLI's (p = 0.0018). High TLI appears to be associated with rapid evolution of breast carcinoma both prior to and following diagnosis and treatment."}
{"id": "PMID:476642", "title": "Methotrexate-induced changes in the levels of 1-beta-D-arabinofuranosylcytosine triphosphate in L1210 cells.", "content": "Treatment with methotrexate (MTX) plus 1-beta-D-arabinofuranosylcytosine (ara-C) on Days 1, 4, and 7 after i.p. inoculation of L1210 ascites cells was more effective than treatment with one drug on Days 1, 4, and 7 followed by the second drug on Days 2, 5, and 8. Simultaneous treatment with both drugs was associated with a retention of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate (ara-CTP) but no increase in the activity of deoxycytidine kinase in L1210 cells, whereas pretreatment with MTX 24 hr before the administration of ara-C was associated with approximately 2-fold increases in the level of ara-CTP and of deoxycytidine kinase in L1210 cells. However, from 5 hr after treatment with ara-C, higher levels of ara-CTP were observed in L1210 cells treated simultaneously with both drugs than in cells from animals pretreated with MTX 24 hr before treatment with ara-C. The superiority of simultaneous treatment over sequential treatment and the synergism between MTX and ara-C, previously reported for this schedule of simultaneous treatment, are attributed in part to the MTX-induced retention of ara-CTP and the increased exposure of L1210 cells to ara-CTP that results from the slower clearance of ara-CTP.", "contents": "Methotrexate-induced changes in the levels of 1-beta-D-arabinofuranosylcytosine triphosphate in L1210 cells. Treatment with methotrexate (MTX) plus 1-beta-D-arabinofuranosylcytosine (ara-C) on Days 1, 4, and 7 after i.p. inoculation of L1210 ascites cells was more effective than treatment with one drug on Days 1, 4, and 7 followed by the second drug on Days 2, 5, and 8. Simultaneous treatment with both drugs was associated with a retention of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate (ara-CTP) but no increase in the activity of deoxycytidine kinase in L1210 cells, whereas pretreatment with MTX 24 hr before the administration of ara-C was associated with approximately 2-fold increases in the level of ara-CTP and of deoxycytidine kinase in L1210 cells. However, from 5 hr after treatment with ara-C, higher levels of ara-CTP were observed in L1210 cells treated simultaneously with both drugs than in cells from animals pretreated with MTX 24 hr before treatment with ara-C. The superiority of simultaneous treatment over sequential treatment and the synergism between MTX and ara-C, previously reported for this schedule of simultaneous treatment, are attributed in part to the MTX-induced retention of ara-CTP and the increased exposure of L1210 cells to ara-CTP that results from the slower clearance of ara-CTP."}
{"id": "PMID:476643", "title": "Inhibition of human melanoma colony formation by retinoids.", "content": "We studied the effects of retinoids on the in vitro survival of melanoma colony-forming cells in biopsies obtained from ten patients with metastatic melanoma. The results indicate that specific retinoids reduce the ability of fresh human melanoma cells to form colonies in soft agar. The retinoids studied had differential effects on the survival of clonogenic melanoma cells, and these effects vary from patient to patient. The data provide support for the clinical trial of selected retinoids in micrometastatic and advanced melanoma.", "contents": "Inhibition of human melanoma colony formation by retinoids. We studied the effects of retinoids on the in vitro survival of melanoma colony-forming cells in biopsies obtained from ten patients with metastatic melanoma. The results indicate that specific retinoids reduce the ability of fresh human melanoma cells to form colonies in soft agar. The retinoids studied had differential effects on the survival of clonogenic melanoma cells, and these effects vary from patient to patient. The data provide support for the clinical trial of selected retinoids in micrometastatic and advanced melanoma."}
{"id": "PMID:476644", "title": "Isoenzymes of beta-hexosaminidase from normal rat colon and colonic carcinoma.", "content": "N-Acetyl-beta-D-hexosaminidase isolated from normal rat colon was compared to that obtained from a transplantable rat colonic carcinoma. Levels of total hexosaminidase from purified epithelial cells of normal colon were similar to those from purified malignant cells from the transplantable tumor. Cultured malignant cells had significantly higher levels of activity than did freshly purified tumor cells. Isoelectric focusing of hexosaminidase from normal rat colon indicated approximately equal amounts of A (pI 5.0 ) and B (pI 8.1) isoenzyme activity. The B isoenzyme (normal cell) was more stable to heat inactivation than was the A isoenzyme and had significantly higher activity at low pH's. In contrast, the B isoenzyme from the tumor was relatively unstable to heat and low pH.", "contents": "Isoenzymes of beta-hexosaminidase from normal rat colon and colonic carcinoma. N-Acetyl-beta-D-hexosaminidase isolated from normal rat colon was compared to that obtained from a transplantable rat colonic carcinoma. Levels of total hexosaminidase from purified epithelial cells of normal colon were similar to those from purified malignant cells from the transplantable tumor. Cultured malignant cells had significantly higher levels of activity than did freshly purified tumor cells. Isoelectric focusing of hexosaminidase from normal rat colon indicated approximately equal amounts of A (pI 5.0 ) and B (pI 8.1) isoenzyme activity. The B isoenzyme (normal cell) was more stable to heat inactivation than was the A isoenzyme and had significantly higher activity at low pH's. In contrast, the B isoenzyme from the tumor was relatively unstable to heat and low pH."}
{"id": "PMID:476645", "title": "Effects of macromomycin on the ultrastructure and biological properties of cultured mammalian cells.", "content": "Macromomycin is shown to inhibit the biosynthesis of RNA, DNA, and protein in cultured cells of KB, HBL-100, SW-613, MCF-7, and A1Ab. There was no substantial increase in cell numbers in cultures containing macromomycin (5 microgram/ml), but after 24 to 48 hr the cells were two to three times the diameter of control cells with concomitant increase in cell protein. The ultrastructural changes induced by macromomycin in KB cells demonstrate an increase in nuclear size without similar changes in the size of other cytoplasmic subcellular units. It was of interest to note the general proliferation of cellular organelles and the increased occurrence of annulate lamellae followed, after prolonged treatment, by the appearance of larger numbers of lipid droplets and lysosomes; vacuoles developed to a significant extent after the cells detached from the monolayer. A1Ab cells show ultrastructural changes similar to those of KB cells when treated with macromomycin.", "contents": "Effects of macromomycin on the ultrastructure and biological properties of cultured mammalian cells. Macromomycin is shown to inhibit the biosynthesis of RNA, DNA, and protein in cultured cells of KB, HBL-100, SW-613, MCF-7, and A1Ab. There was no substantial increase in cell numbers in cultures containing macromomycin (5 microgram/ml), but after 24 to 48 hr the cells were two to three times the diameter of control cells with concomitant increase in cell protein. The ultrastructural changes induced by macromomycin in KB cells demonstrate an increase in nuclear size without similar changes in the size of other cytoplasmic subcellular units. It was of interest to note the general proliferation of cellular organelles and the increased occurrence of annulate lamellae followed, after prolonged treatment, by the appearance of larger numbers of lipid droplets and lysosomes; vacuoles developed to a significant extent after the cells detached from the monolayer. A1Ab cells show ultrastructural changes similar to those of KB cells when treated with macromomycin."}
{"id": "PMID:476649", "title": "Penetration of 3-deazauridine into human brain, intracerebral tumor, and cerebrospinal fluid.", "content": "The antitumor agent 3-deazauridine (DAU) was administered rapidly to four patients before surgical removal of intracerebral tumor. Tumor, adjacent brain tissue, and temporalis muscle were assayed for DAU by high-pressure liquid chromatography. DAU penetrated comparably into tumor, brain, and muscle; in one patient, tissue concentrations were higher than concurrent plasma concentrations. The active metabolite 3-deazauridine 5'-triphosphate was quantitated in one tumor sample and greatly exceeded its Ki for cytidine 5'-triphosphate synthetase. DAU was also present in autopsy brain specimens from two patients treated shortly antemortem. Cerebrospinal fluid concentrations were 22.1 and 59.0%, respectively, of concurrent plasma concentrations during continuous infusion of DAU in two patients. Cerebrospinal fluid concentration was 3.1 microgram/ml 2 hr after a 30-min infusion of 1.5 g of drug per sq m and fell to 1.9 microgram/ml at 16 hr. Thus, DAU is capable of penetrating into intracerebral tumor, brain, and cerebrospinal fluid and is worthy of investigation in the treatment of intracerebral and meningeal neoplasms.", "contents": "Penetration of 3-deazauridine into human brain, intracerebral tumor, and cerebrospinal fluid. The antitumor agent 3-deazauridine (DAU) was administered rapidly to four patients before surgical removal of intracerebral tumor. Tumor, adjacent brain tissue, and temporalis muscle were assayed for DAU by high-pressure liquid chromatography. DAU penetrated comparably into tumor, brain, and muscle; in one patient, tissue concentrations were higher than concurrent plasma concentrations. The active metabolite 3-deazauridine 5'-triphosphate was quantitated in one tumor sample and greatly exceeded its Ki for cytidine 5'-triphosphate synthetase. DAU was also present in autopsy brain specimens from two patients treated shortly antemortem. Cerebrospinal fluid concentrations were 22.1 and 59.0%, respectively, of concurrent plasma concentrations during continuous infusion of DAU in two patients. Cerebrospinal fluid concentration was 3.1 microgram/ml 2 hr after a 30-min infusion of 1.5 g of drug per sq m and fell to 1.9 microgram/ml at 16 hr. Thus, DAU is capable of penetrating into intracerebral tumor, brain, and cerebrospinal fluid and is worthy of investigation in the treatment of intracerebral and meningeal neoplasms."}
{"id": "PMID:476650", "title": "A selective effect of methylglyoxal-bis(guanylhydrazone) on the synthesis of mitochondrial DNA of cultured L1210 leukemia cells.", "content": "Methylglyoxal-bis(guanylhydrazone) (MGBG) is a polycationic drug which is useful in the chemotherapy of lymphoid and myeloid proliferative disorders. The drug has recently been shown to produce selective ultrastructural damage to the mitochondria of proliferating cell populations. It is important to understand the molecular basis for this action, since it may be related to the known ability of MGBG to block polyamine biosynthesis. Accordingly, the effect of MGBG treatment on the incorporation of [3H]thymidine into both mitochondrial and nuclear DNA has been examined. Exponentially growing L1210 leukemia cells were prelabeled with [14C]thymidine, treated with MGBG for 1.5 to 16 hr, and then pulse labeled with [3H]-thymidine. Incorporation of [3H]thymidine into mitochondrial DNA was selectively inhibited at 5 hr with concentrations of 1 to 10 microM MGBG. Nuclear DNA, however, was not similarly affected until 8 to 11 hr of drug treatment. Dye-CsCl gradients of mitochondrial DNA indicated that the inhibition of synthesis occurred in replicative forms of circular DNA. Uptake studies excluded the possibility of drug interference with cellular uptake of thymidine. Ultrastructural studies revealed a very close correlation between the dose-response curve for mitochondrial damage and that for MGBG inhibition of mitochondrial DNA synthesis. This correlation suggests a direct cause-and-effect relationship between inhibition of mitochondrial DNA synthesis and ultrastructural damage, but the possibility of both phenomena being related to another action by the drug, such as inhibition of polyamine biosynthesis, or a drug effect on mitochondrial function, must also be considered.", "contents": "A selective effect of methylglyoxal-bis(guanylhydrazone) on the synthesis of mitochondrial DNA of cultured L1210 leukemia cells. Methylglyoxal-bis(guanylhydrazone) (MGBG) is a polycationic drug which is useful in the chemotherapy of lymphoid and myeloid proliferative disorders. The drug has recently been shown to produce selective ultrastructural damage to the mitochondria of proliferating cell populations. It is important to understand the molecular basis for this action, since it may be related to the known ability of MGBG to block polyamine biosynthesis. Accordingly, the effect of MGBG treatment on the incorporation of [3H]thymidine into both mitochondrial and nuclear DNA has been examined. Exponentially growing L1210 leukemia cells were prelabeled with [14C]thymidine, treated with MGBG for 1.5 to 16 hr, and then pulse labeled with [3H]-thymidine. Incorporation of [3H]thymidine into mitochondrial DNA was selectively inhibited at 5 hr with concentrations of 1 to 10 microM MGBG. Nuclear DNA, however, was not similarly affected until 8 to 11 hr of drug treatment. Dye-CsCl gradients of mitochondrial DNA indicated that the inhibition of synthesis occurred in replicative forms of circular DNA. Uptake studies excluded the possibility of drug interference with cellular uptake of thymidine. Ultrastructural studies revealed a very close correlation between the dose-response curve for mitochondrial damage and that for MGBG inhibition of mitochondrial DNA synthesis. This correlation suggests a direct cause-and-effect relationship between inhibition of mitochondrial DNA synthesis and ultrastructural damage, but the possibility of both phenomena being related to another action by the drug, such as inhibition of polyamine biosynthesis, or a drug effect on mitochondrial function, must also be considered."}
{"id": "PMID:476651", "title": "Enhancement of the chromosome-damaging action of ascorbate by transition metals.", "content": "Freshly prepared ascorbate inhibited mitosis and induced chromosome aberrations in cultured Chinese hamster ovary cells. Cu(II) and Mn(II) (10(-4) or 10(-5) M) enhanced both actions. Fe(II) and Fe(III) (10(-4) or 10(-5) M) reduced or abolished the mitosis-inhibiting action of ascorbate. At 10(-4) M, Fe(II) and Fe(III) strongly enhanced the chromosome-damaging capacity of ascorbate. Up to 100% of all examined metaphase plates had multiple chromosome exchanges or breaks. Since the cytostatic and clastogenic effect of ascorbate of H2O2 to induce chromosome aberrations was examined. H2O2 and a H2O2: Fe(II) mixture (Fenton reagent) induced chromosome breaks and exchanges but to a lesser degree than did ascorbate: Cu(II), Mn(II), Fe(II), or Fe(III) mixtures. Whether the strong chromosome damaging capacity of ascorbate plus transition metals as seen in the in vitro test system poses a health hazard only properly designed in vivo studies can reveal.", "contents": "Enhancement of the chromosome-damaging action of ascorbate by transition metals. Freshly prepared ascorbate inhibited mitosis and induced chromosome aberrations in cultured Chinese hamster ovary cells. Cu(II) and Mn(II) (10(-4) or 10(-5) M) enhanced both actions. Fe(II) and Fe(III) (10(-4) or 10(-5) M) reduced or abolished the mitosis-inhibiting action of ascorbate. At 10(-4) M, Fe(II) and Fe(III) strongly enhanced the chromosome-damaging capacity of ascorbate. Up to 100% of all examined metaphase plates had multiple chromosome exchanges or breaks. Since the cytostatic and clastogenic effect of ascorbate of H2O2 to induce chromosome aberrations was examined. H2O2 and a H2O2: Fe(II) mixture (Fenton reagent) induced chromosome breaks and exchanges but to a lesser degree than did ascorbate: Cu(II), Mn(II), Fe(II), or Fe(III) mixtures. Whether the strong chromosome damaging capacity of ascorbate plus transition metals as seen in the in vitro test system poses a health hazard only properly designed in vivo studies can reveal."}
{"id": "PMID:476653", "title": "Fate of nickel subsulfide during carcinogenesis studied by autoradiography and X-ray powder diffraction.", "content": "Sarcomas in mice were induced by i.m. and s.c. administration of 63Ni- and 35S-labeled nickel subsulfide (Ni3S2), and the fate of the Ni3S2 was studied in tumors and normal tissues during carcinogenesis. Whole-body autoradiography showed a gradual loss of solubilized 63Ni and 35S radioactivity from the site of injection. There was also a loss of nonsolubilized dust particles which appeared to be phagocytized by reticuloendothelial cells in the liver, spleen, and regional lymph nodes. Microautoradiography showed that the totally dominating radioactivity within both the 63Ni3S2- and the Ni3(35)S2-induced tumors was associated with dust particles. There was no specific or excessive localization of solubilized radioactivity in the tumors or in metastases (when present). Two patterns of localization of dust particles within the tumors were observed: one with particles concentrated in a central part of the tumor and one with the particles present in the periphery of the tumor. X-ray powder diffraction of the insoluble crystalline material in the tumors indicated that a conversion of the alpha Ni3S2 to alpha Ni7S6 and beta NiS had occurred.", "contents": "Fate of nickel subsulfide during carcinogenesis studied by autoradiography and X-ray powder diffraction. Sarcomas in mice were induced by i.m. and s.c. administration of 63Ni- and 35S-labeled nickel subsulfide (Ni3S2), and the fate of the Ni3S2 was studied in tumors and normal tissues during carcinogenesis. Whole-body autoradiography showed a gradual loss of solubilized 63Ni and 35S radioactivity from the site of injection. There was also a loss of nonsolubilized dust particles which appeared to be phagocytized by reticuloendothelial cells in the liver, spleen, and regional lymph nodes. Microautoradiography showed that the totally dominating radioactivity within both the 63Ni3S2- and the Ni3(35)S2-induced tumors was associated with dust particles. There was no specific or excessive localization of solubilized radioactivity in the tumors or in metastases (when present). Two patterns of localization of dust particles within the tumors were observed: one with particles concentrated in a central part of the tumor and one with the particles present in the periphery of the tumor. X-ray powder diffraction of the insoluble crystalline material in the tumors indicated that a conversion of the alpha Ni3S2 to alpha Ni7S6 and beta NiS had occurred."}
{"id": "PMID:476654", "title": "Immune elimination of host fibroblasts for the cultivation of human tumors transplanted into nude mice.", "content": "We report here a useful method for the isolation and cultivation of human tumor cells in vitro from human tumors grown in nude mice. A rabbit was immunized with spleen cells obtained from adult nude mice. The rabbit antiserum in the presence of complement effectively killed cultured cells derived from various mouse tissues, but it was not cytotoxic to cultured cells from human tissues including tumors. When mixed cultures consisting of human tumor cells and nude mouse fibroblasts were treated with the antiserum and complement, the nude mouse fibroblasts were completely removed from the cultures, and the human tumor cells could be propagated without noticeable changes in morphological features. Primary cultures of heterotransplanted human tumors grown in nude mice were also successfully treated, resulting in the ultimate elimination of fibroblastic cells derived from the stroma of the tumor. The functional properties of the tumor cells (production of human chorionic gonadotropin by choriocarcinoma cells and production of carcinoembryonic antigens by pancreas carcinoma cells) were also maintained after the antiserum treatment.", "contents": "Immune elimination of host fibroblasts for the cultivation of human tumors transplanted into nude mice. We report here a useful method for the isolation and cultivation of human tumor cells in vitro from human tumors grown in nude mice. A rabbit was immunized with spleen cells obtained from adult nude mice. The rabbit antiserum in the presence of complement effectively killed cultured cells derived from various mouse tissues, but it was not cytotoxic to cultured cells from human tissues including tumors. When mixed cultures consisting of human tumor cells and nude mouse fibroblasts were treated with the antiserum and complement, the nude mouse fibroblasts were completely removed from the cultures, and the human tumor cells could be propagated without noticeable changes in morphological features. Primary cultures of heterotransplanted human tumors grown in nude mice were also successfully treated, resulting in the ultimate elimination of fibroblastic cells derived from the stroma of the tumor. The functional properties of the tumor cells (production of human chorionic gonadotropin by choriocarcinoma cells and production of carcinoembryonic antigens by pancreas carcinoma cells) were also maintained after the antiserum treatment."}
{"id": "PMID:476658", "title": "Enhancement of the nuclear reactivity of alkylating agents by prednisolone.", "content": "We have compared the nuclear reactions of the alkylating agents chlorambucil, melphalan, and cyclophosphamide used alone, with those produced when the same drugs were used in combination with the corticosteroid prednisolone. Studies were performed in vivo in Wistar rats bearing ascites forms of either the Yoshida sarcoma or the Walker carcinosarcoma. Strains of both tumor lines, which were sensitive to alkylating agents, showed loss of condensed chromatin, increased phosphorylation of nuclear proteins, and increased DNA cross-linking after exposure to the alkylating agents alone; these changes were generally enhanced in the presence of prednisolone. When paired tumor lines which exhibited acquired resistance to alkylating agents were studied, no nuclear changes were detected when individual alkylating agents were given alone. However, in combination with prednisolone, each of the three alkylating agents produced loss of condensed chromatin, increased nuclear protein phosphorylation, and DNA cross-linking. These chromatin changes were associated with tumor cell death and may determine drug sensitivity in the tumors studied.", "contents": "Enhancement of the nuclear reactivity of alkylating agents by prednisolone. We have compared the nuclear reactions of the alkylating agents chlorambucil, melphalan, and cyclophosphamide used alone, with those produced when the same drugs were used in combination with the corticosteroid prednisolone. Studies were performed in vivo in Wistar rats bearing ascites forms of either the Yoshida sarcoma or the Walker carcinosarcoma. Strains of both tumor lines, which were sensitive to alkylating agents, showed loss of condensed chromatin, increased phosphorylation of nuclear proteins, and increased DNA cross-linking after exposure to the alkylating agents alone; these changes were generally enhanced in the presence of prednisolone. When paired tumor lines which exhibited acquired resistance to alkylating agents were studied, no nuclear changes were detected when individual alkylating agents were given alone. However, in combination with prednisolone, each of the three alkylating agents produced loss of condensed chromatin, increased nuclear protein phosphorylation, and DNA cross-linking. These chromatin changes were associated with tumor cell death and may determine drug sensitivity in the tumors studied."}
{"id": "PMID:476661", "title": "Prolonged retention of estradiol by human breast cancer cells in tissue culture.", "content": "Conditions are described under which prolonged estradiol retention and estrogenic activity are observed in human breast cancer cells in tissue culture. The cells were incubated for three hr with a physiological concentration of [3H]estradiol (3 to 5 nM) and then were washed with 3 successive exchanges of medium 3, 17, and 24 or 48 hr following incubation with [3H]estradiol. The total wash period was 78 hr. The following parameters were monitored to assess the duration of estrogen action in MCF-7 human breast cancer cells in tissue culture; (a) the concentration of [3H]estradiol and [3H]estradiol metabolites in the media washes; (b) the intracellular concentration of [3H]estradiol and [3H]estradiol metabolites; and (c) the time course of estradiol-enhanced rates of radiolabeled thymidine incorporation. The [3H]estradiol concentration in the final medium wash was approximately 0.05 nM. The total intracellular concentration of tritium was about 50 nM prior to wash and 9 nM following 78 hr of wash. The intracellular concentration of specifically bound [3H]estradiol was initially 18 nM, and after 78 hr of wash, it was 2.8 nM. After 48 hr of wash, nearly all specifically bound [3H]estradiol was present in the nucleus. Following incubation of the cells with 5 nM estradiol and an identical wash procedure, estrogenic activity as measured by a stimulation of thymidine incorporation was observed throughout the 78 hr monitored. When 10(-6) M tamoxifen or 10(-7) M unlabeled estradiol was included in the medium washes, the washout of nonspecific binding was unaffected; however, specifically bound [3H]estradiol was essentially eliminated within 24 hr. When bovine serum albumin was included in the medium washes, total, nonspecific, and specific [3H]estradiol binding was reduced in a parallel and dose-dependent fashion. After 48 hr, cells washed with medium containing 3.5 or 7% bovine serum albumin contained one-tenth of the [3H]estradiol present in cells washed with medium alone. We conclude that medium exchanges alone do not effectively remove estradiol from MCF-7 cells, and suggest that estrogen retention by estrogen-responsive cells may mask in vitro assessments of such responsiveness in this and other systems. Inclusion of bovine serum albumin in the washes may alleviate this problem.", "contents": "Prolonged retention of estradiol by human breast cancer cells in tissue culture. Conditions are described under which prolonged estradiol retention and estrogenic activity are observed in human breast cancer cells in tissue culture. The cells were incubated for three hr with a physiological concentration of [3H]estradiol (3 to 5 nM) and then were washed with 3 successive exchanges of medium 3, 17, and 24 or 48 hr following incubation with [3H]estradiol. The total wash period was 78 hr. The following parameters were monitored to assess the duration of estrogen action in MCF-7 human breast cancer cells in tissue culture; (a) the concentration of [3H]estradiol and [3H]estradiol metabolites in the media washes; (b) the intracellular concentration of [3H]estradiol and [3H]estradiol metabolites; and (c) the time course of estradiol-enhanced rates of radiolabeled thymidine incorporation. The [3H]estradiol concentration in the final medium wash was approximately 0.05 nM. The total intracellular concentration of tritium was about 50 nM prior to wash and 9 nM following 78 hr of wash. The intracellular concentration of specifically bound [3H]estradiol was initially 18 nM, and after 78 hr of wash, it was 2.8 nM. After 48 hr of wash, nearly all specifically bound [3H]estradiol was present in the nucleus. Following incubation of the cells with 5 nM estradiol and an identical wash procedure, estrogenic activity as measured by a stimulation of thymidine incorporation was observed throughout the 78 hr monitored. When 10(-6) M tamoxifen or 10(-7) M unlabeled estradiol was included in the medium washes, the washout of nonspecific binding was unaffected; however, specifically bound [3H]estradiol was essentially eliminated within 24 hr. When bovine serum albumin was included in the medium washes, total, nonspecific, and specific [3H]estradiol binding was reduced in a parallel and dose-dependent fashion. After 48 hr, cells washed with medium containing 3.5 or 7% bovine serum albumin contained one-tenth of the [3H]estradiol present in cells washed with medium alone. We conclude that medium exchanges alone do not effectively remove estradiol from MCF-7 cells, and suggest that estrogen retention by estrogen-responsive cells may mask in vitro assessments of such responsiveness in this and other systems. Inclusion of bovine serum albumin in the washes may alleviate this problem."}
{"id": "PMID:476662", "title": "Arming of normal leukocytes with sera from patients with adenocarcinoma of the prostate.", "content": "A tumor-associated antigen-induced leukocyte adherence inhibition assay was used to evaluate the effect of serum from patients with adenocarcinoma of the prostate on the antitumor reactivity of normal leukocytes. Peripheral blood leukocytes from 53 normal (control) subjects were armed with serum from 22 patients with localized (Stage A) and metastatic (Stage D) prostatic cancer and reacted with allogenic extract of malignant prostate as specific tumor-associated antigen. Leukocytes pre-treated with serum from patients with Stage A cancer show significantly stronger responses to malignant prostate than do those pretreated with serum from patients with Stage D cancer, which induced little or no response. This may be attributed to an \"arming factor\" present in the sera of patients with an initial stage of prostatic cancer which appears to be capable of sensitizing normal leukocytes and making them specifically reactive to tumor extract. The specificity of arming with individual and pooled patient's sera was delineated by the use of extracts from other genitourinary tumors.", "contents": "Arming of normal leukocytes with sera from patients with adenocarcinoma of the prostate. A tumor-associated antigen-induced leukocyte adherence inhibition assay was used to evaluate the effect of serum from patients with adenocarcinoma of the prostate on the antitumor reactivity of normal leukocytes. Peripheral blood leukocytes from 53 normal (control) subjects were armed with serum from 22 patients with localized (Stage A) and metastatic (Stage D) prostatic cancer and reacted with allogenic extract of malignant prostate as specific tumor-associated antigen. Leukocytes pre-treated with serum from patients with Stage A cancer show significantly stronger responses to malignant prostate than do those pretreated with serum from patients with Stage D cancer, which induced little or no response. This may be attributed to an \"arming factor\" present in the sera of patients with an initial stage of prostatic cancer which appears to be capable of sensitizing normal leukocytes and making them specifically reactive to tumor extract. The specificity of arming with individual and pooled patient's sera was delineated by the use of extracts from other genitourinary tumors."}
{"id": "PMID:476663", "title": "Inhibition of phorbol myristate acetate and phytohemagglutinin stimulation of human lymphocytes by retinol.", "content": "Retinol (vitamin A) in concentrations above (5 microgram/ml inhibits the stimulation of human lymphocytes by phytohemagglutinin and phorbol myristate acetate. This is in contrast to bovine lymphocytes in which retinol inhibits only phorbol myristate acetate stimulation of lymphocytes. The cause of the inhibition does not appear to be due to a direct toxic action because the viability and ultrastructure of lymphocytes exposed to a concentration of 20 microgram/ml for 72 hr were normal. The mechanism of action of the retinol is not known, but it may be mediated by a retinol-binding protein in the cells.", "contents": "Inhibition of phorbol myristate acetate and phytohemagglutinin stimulation of human lymphocytes by retinol. Retinol (vitamin A) in concentrations above (5 microgram/ml inhibits the stimulation of human lymphocytes by phytohemagglutinin and phorbol myristate acetate. This is in contrast to bovine lymphocytes in which retinol inhibits only phorbol myristate acetate stimulation of lymphocytes. The cause of the inhibition does not appear to be due to a direct toxic action because the viability and ultrastructure of lymphocytes exposed to a concentration of 20 microgram/ml for 72 hr were normal. The mechanism of action of the retinol is not known, but it may be mediated by a retinol-binding protein in the cells."}
{"id": "PMID:476664", "title": "An estimate of the maximum in vivo covalent binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to rat liver protein, ribosomal RNA, and DNA.", "content": "2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an extraordinarily potent toxin, has recently been found to be a potent carcinogen producing mucosal, lung, and liver tumors in female rats. In light of this carcinogenicity, we reexamined the in vivo covalent binding of [3H]TCDD to rat liver macromolecules. Immature Sprague-Dawley rats, receiving [3H]TCDD (0.87 mCi/kg; specific activity, 39 Ci/mmol) concentrated 18 to 64% of the total administered dose in their livers, but virtually all of this radioactivity (greater than 99.9%) was extractable. The maximum unextractable radioactivity was: protein, 60 pmol TCDD per mol of amino acid residue; rRNA, 12 pmol TCDD per mol of nucleotide residue; and DNA, 6 pmol TCDD per mol of nucleotide residue. If one assumes that this small residual amount of radioactivity represents covalent binding, this binding is 4 to 6 orders of magnitude lower than that of most chemical carcinogens, and the binding to DNA is equivalent to one molecule of TCDD per DNA, equivalent to 35 cells. The results suggest it is unlikely that TCDD-induced oncogenesis is through a mechanism of covalent binding to DNA and somatic mutation.", "contents": "An estimate of the maximum in vivo covalent binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to rat liver protein, ribosomal RNA, and DNA. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an extraordinarily potent toxin, has recently been found to be a potent carcinogen producing mucosal, lung, and liver tumors in female rats. In light of this carcinogenicity, we reexamined the in vivo covalent binding of [3H]TCDD to rat liver macromolecules. Immature Sprague-Dawley rats, receiving [3H]TCDD (0.87 mCi/kg; specific activity, 39 Ci/mmol) concentrated 18 to 64% of the total administered dose in their livers, but virtually all of this radioactivity (greater than 99.9%) was extractable. The maximum unextractable radioactivity was: protein, 60 pmol TCDD per mol of amino acid residue; rRNA, 12 pmol TCDD per mol of nucleotide residue; and DNA, 6 pmol TCDD per mol of nucleotide residue. If one assumes that this small residual amount of radioactivity represents covalent binding, this binding is 4 to 6 orders of magnitude lower than that of most chemical carcinogens, and the binding to DNA is equivalent to one molecule of TCDD per DNA, equivalent to 35 cells. The results suggest it is unlikely that TCDD-induced oncogenesis is through a mechanism of covalent binding to DNA and somatic mutation."}
{"id": "PMID:476665", "title": "Early effects of dimethylnitrosamine on protein chain initiation and postmicrosomal polyadenylic acid-containing RNA content in mouse liver.", "content": "A mouse liver S-30 system was used to study the early effects of dimethylnitrosamine (DMNA) on polypeptide chain initiation and messenger RNA content. The inhibition of protein synthesis after DMNA administration was associated with a reduced capacity of the S-30 system to form 80S ribosomal initiation complexes. The binding of formylatable methionyl transfer RNA to polysomes was also depressed. The initiation defect was detectable in the assay system slightly later than the decrease in protein synthesis. Addition of mRNA stimulated both translation and 80S initiation complex formation but could not fully restore the activity of the S-30 system from DMNA-treated mice. A loss of poly(A)+ RNA from the postmicrosomal subfraction of the S-30 fraction was observed as early as 15 min after DMNA administration. Later, polyriboadenylic acid also decreased in the microsomal fraction. Monosomes accumulating in response to DMNA treatment were deficient in mRNA as measured by polyriboadenylic acid analysis. Conversely, the proportion of polyriboadenylic acid in the remaining polysomes increased, indicating that the mRNA had become less densely occupied with ribosomes.", "contents": "Early effects of dimethylnitrosamine on protein chain initiation and postmicrosomal polyadenylic acid-containing RNA content in mouse liver. A mouse liver S-30 system was used to study the early effects of dimethylnitrosamine (DMNA) on polypeptide chain initiation and messenger RNA content. The inhibition of protein synthesis after DMNA administration was associated with a reduced capacity of the S-30 system to form 80S ribosomal initiation complexes. The binding of formylatable methionyl transfer RNA to polysomes was also depressed. The initiation defect was detectable in the assay system slightly later than the decrease in protein synthesis. Addition of mRNA stimulated both translation and 80S initiation complex formation but could not fully restore the activity of the S-30 system from DMNA-treated mice. A loss of poly(A)+ RNA from the postmicrosomal subfraction of the S-30 fraction was observed as early as 15 min after DMNA administration. Later, polyriboadenylic acid also decreased in the microsomal fraction. Monosomes accumulating in response to DMNA treatment were deficient in mRNA as measured by polyriboadenylic acid analysis. Conversely, the proportion of polyriboadenylic acid in the remaining polysomes increased, indicating that the mRNA had become less densely occupied with ribosomes."}
{"id": "PMID:476666", "title": "Agglutination of bladder cells by concanavalin A during the early phase of treatment of rats with N-butyl-N-(4-hydroxybutyl)nitrosamine.", "content": "N-Butyl-N-(4-hydroxybutyl)nitrosamine was given to male Wistar rats at a dose of 0.05% in the drinking water for one to five weeks, and agglutination of cell isolated from their bladder by concanavalin A (Con A) was determined at intervals during and after treatment. Mucosal cells were isolated from everted bladder by ethylenediaminetetraacetate treatment and sonication. As early as one week after the start of treatment, Con A caused some agglutination of isolated bladder cells, and this agglutination increased with time, reaching an almost constant value from the third week. Con A agglutination of bladder cells induced by N-butyl-N-(4-hydroxybutyl)nitrosamine treatment for only one week appeared to be irreversible, and it was still observed two weeks after the end of treatment. Scanning electron microscopy showed that microvilli developed on the luminal surface of mucosal cells in situ at the time when the isolated cells became agglutinable with Con A. Measurement of agglutinability of isolated bladder cells with Con A might be a useful way of detecting very early changes in bladder carcinogenesis.", "contents": "Agglutination of bladder cells by concanavalin A during the early phase of treatment of rats with N-butyl-N-(4-hydroxybutyl)nitrosamine. N-Butyl-N-(4-hydroxybutyl)nitrosamine was given to male Wistar rats at a dose of 0.05% in the drinking water for one to five weeks, and agglutination of cell isolated from their bladder by concanavalin A (Con A) was determined at intervals during and after treatment. Mucosal cells were isolated from everted bladder by ethylenediaminetetraacetate treatment and sonication. As early as one week after the start of treatment, Con A caused some agglutination of isolated bladder cells, and this agglutination increased with time, reaching an almost constant value from the third week. Con A agglutination of bladder cells induced by N-butyl-N-(4-hydroxybutyl)nitrosamine treatment for only one week appeared to be irreversible, and it was still observed two weeks after the end of treatment. Scanning electron microscopy showed that microvilli developed on the luminal surface of mucosal cells in situ at the time when the isolated cells became agglutinable with Con A. Measurement of agglutinability of isolated bladder cells with Con A might be a useful way of detecting very early changes in bladder carcinogenesis."}
{"id": "PMID:476667", "title": "Distribution of radiolabeled alloantibodies in mice bearing 3-methylcholanthrene-induced sarcoma.", "content": "The distribution of purified 125I-labeled alloantibodies, prepared from the serum of DBA/2J mice obtained after immunization with C3H/HeJ spleen cells, was studied in immunosuppressed DBA/2J mice bearing either allogeneic C3H/HeJ 3-methylcholanthrene sarcomas growing s.c. or syngeneic SaD2 3-methylcholanthrene sarcomas. Once purified radiolabeled antibody was isolated from 125I-labeled immune gamma-globulin by a single adsorption onto C3H/HeJ RBC and elution from stroma prepared from these cells, by using 0.1 M glycine buffer (pH 3.0). Twice-purified alloantibody was then produced by Bio-Gel P-200 or Sephadex G-200 gel filtration chromatography or DEAE A-50 ion-exchange chromatography. In vitro, such purified antibodies bound specifically to C3H/HeJ RBC. In vivo, they localized to a significant extent following i.p. injection, preferentially in C3H/HeJ 3-methylcholanthrene sarcomas (4.4 to 8.9% of the injected dose per g of tumor equal to 1% of mouse weight), with mean tumor/blood ratios of 4.0 to 7.8, at 24 or 48 hr after injection. The percentage of injected dose localized in tumor and the tumor/blood ratio did not show significant differences with respect to time or method of antibody purification. Normal tissue/blood ratios in C3H/HeJ or SaD2 sarcoma-bearing mice were less than 0.9. The tumor/blood ratios in SaD2 sarcomas were approximately 0.6. Injection of 131I-labeled normal DBA/2J gamma-globulin resulted in normal tissue/blood and tumor/blood ratios of less than 0.9 in C3H/HeJ tumor-bearing mice.", "contents": "Distribution of radiolabeled alloantibodies in mice bearing 3-methylcholanthrene-induced sarcoma. The distribution of purified 125I-labeled alloantibodies, prepared from the serum of DBA/2J mice obtained after immunization with C3H/HeJ spleen cells, was studied in immunosuppressed DBA/2J mice bearing either allogeneic C3H/HeJ 3-methylcholanthrene sarcomas growing s.c. or syngeneic SaD2 3-methylcholanthrene sarcomas. Once purified radiolabeled antibody was isolated from 125I-labeled immune gamma-globulin by a single adsorption onto C3H/HeJ RBC and elution from stroma prepared from these cells, by using 0.1 M glycine buffer (pH 3.0). Twice-purified alloantibody was then produced by Bio-Gel P-200 or Sephadex G-200 gel filtration chromatography or DEAE A-50 ion-exchange chromatography. In vitro, such purified antibodies bound specifically to C3H/HeJ RBC. In vivo, they localized to a significant extent following i.p. injection, preferentially in C3H/HeJ 3-methylcholanthrene sarcomas (4.4 to 8.9% of the injected dose per g of tumor equal to 1% of mouse weight), with mean tumor/blood ratios of 4.0 to 7.8, at 24 or 48 hr after injection. The percentage of injected dose localized in tumor and the tumor/blood ratio did not show significant differences with respect to time or method of antibody purification. Normal tissue/blood ratios in C3H/HeJ or SaD2 sarcoma-bearing mice were less than 0.9. The tumor/blood ratios in SaD2 sarcomas were approximately 0.6. Injection of 131I-labeled normal DBA/2J gamma-globulin resulted in normal tissue/blood and tumor/blood ratios of less than 0.9 in C3H/HeJ tumor-bearing mice."}
{"id": "PMID:476668", "title": "Metabolic activation of arylhydroxamic acids by N-O-acyltransferase of rat mammary gland.", "content": "The lactating mammary glands of rats contain an arylhydroxamic acid N,O-acyltransferase that catalyzes the formation of arylamine-substituted nucleic acid on incubation with N-hydroxy-N-2-acetylaminofluorene or N-hydroxy-N-4-acetylaminobiphenyl and transfer RNA. The acyltransferase activity migrates as a single component with a molecular weight of 28,000 on gel filtration on Sephadex G-100. Acyltransferase activities of the lactating mammary glands of Sprague-Dawley animals are approximately twice those of the less susceptible Fischer strain as determined by assay with either hydroxamic acid. The fluorene substrate was 15 times as efficient as the biphenyl compound in promoting adduct formation. Ribosomal RNA adducts formed in vivo after administration of N-hydroxy-N-2-acetylaminofluorene were consistent with an acyltransferase mechanism of activation in that the adducts did not retain the acetyl group.", "contents": "Metabolic activation of arylhydroxamic acids by N-O-acyltransferase of rat mammary gland. The lactating mammary glands of rats contain an arylhydroxamic acid N,O-acyltransferase that catalyzes the formation of arylamine-substituted nucleic acid on incubation with N-hydroxy-N-2-acetylaminofluorene or N-hydroxy-N-4-acetylaminobiphenyl and transfer RNA. The acyltransferase activity migrates as a single component with a molecular weight of 28,000 on gel filtration on Sephadex G-100. Acyltransferase activities of the lactating mammary glands of Sprague-Dawley animals are approximately twice those of the less susceptible Fischer strain as determined by assay with either hydroxamic acid. The fluorene substrate was 15 times as efficient as the biphenyl compound in promoting adduct formation. Ribosomal RNA adducts formed in vivo after administration of N-hydroxy-N-2-acetylaminofluorene were consistent with an acyltransferase mechanism of activation in that the adducts did not retain the acetyl group."}
{"id": "PMID:476669", "title": "beta-Hexosaminidase isoenzymes in tissues, cultured cells, and media from human fetal intestine and colonic adenocarcinoma.", "content": "Isoelectric focusing of tissue homogenates revealed a predominance of beta-hexosaminidase B in colonic adenocarcinoma, whereas beta-hexosaminidase A was greater in paired normal-appearing colonic mucosa from the same patients as well as in a specimen of human fetal colonic mucosa. Because of the recognized cellular heterogeneity of these tissues, our studies were extended to an examination of these isoenzymes in 20 cultured epithelial cell lines derived from human fetal intestine and colonic carcinoma as well as the secreted enzymes in their culture media. While the B:A isoenzyme ratio was higher in human cancer cells as compared to fetal cells, some of the cancer cell lines had a greater proportion of the A isoenzyme. Examination of the isoenzyme profiles in the media of these cells revealed a greater B:A ratio whether of fetal or cancer cell origin. These studies parallel the apparent biological differences of neoplastic colonic epithelium occurring in vivo and are reminiscent of reported oncodevelopmental changes in enzymatic properties present in some malignant tissues. The differential stabilities of these two isoenzymes derived from the culture media of both types of cell lines in vitro may limit their value as markers of human colonic neoplasia.", "contents": "beta-Hexosaminidase isoenzymes in tissues, cultured cells, and media from human fetal intestine and colonic adenocarcinoma. Isoelectric focusing of tissue homogenates revealed a predominance of beta-hexosaminidase B in colonic adenocarcinoma, whereas beta-hexosaminidase A was greater in paired normal-appearing colonic mucosa from the same patients as well as in a specimen of human fetal colonic mucosa. Because of the recognized cellular heterogeneity of these tissues, our studies were extended to an examination of these isoenzymes in 20 cultured epithelial cell lines derived from human fetal intestine and colonic carcinoma as well as the secreted enzymes in their culture media. While the B:A isoenzyme ratio was higher in human cancer cells as compared to fetal cells, some of the cancer cell lines had a greater proportion of the A isoenzyme. Examination of the isoenzyme profiles in the media of these cells revealed a greater B:A ratio whether of fetal or cancer cell origin. These studies parallel the apparent biological differences of neoplastic colonic epithelium occurring in vivo and are reminiscent of reported oncodevelopmental changes in enzymatic properties present in some malignant tissues. The differential stabilities of these two isoenzymes derived from the culture media of both types of cell lines in vitro may limit their value as markers of human colonic neoplasia."}
{"id": "PMID:476670", "title": "The N-hydroxy metabolites of N-methyl-4-aminoazobenzene and related dyes as proximate carcinogens in the rat and mouse.", "content": "The carcinogenicities for rats and mice of N-methyl-4-aminoazobenzene (MAB) and its hepatic microsomal metabolite N-hydroxy-N-methyl-4-aminoazobenzene (N-hydroxy-MAB) were compared under several conditions. N-Ethyl-4-aminoazobenzene, 4-aminoazobenzene, and their N-hydroxy derivatives were also included in some of the assays. About 25% of the rats given MAB or N-hydroxy-MAB (3 to 5 mmol/kg body weight) by stomach tube over a 5-week period developed hepatic tumors by 18 to 22 months. Similarly treated rats subsequently given phenobarbital in the drinking water until the termination of the experiment developed about twice as many hepatic tumors. N-Hydroxy-MAB, administered p.o., but not MAB, also induced multiple papillomas and extensive carcinomas of the forestomach in approximately 50% of the rats. Only low incidence of hepatocellular carcinomas occurred in partially hepatectomized rats given a single i.p. injection of 180 mumol/kg body weight of MAB or N-hydroxy-MAB with or without subsequent administration of phenobarbital. Although repeated s.c. doses of N-benzoyloxy-N-methyl-4-aminoazobenzene induced sarcomas at the injection site in 90% of the rats, only 3 of 20 rats developed sarcomas at the site of s.c. injections of N-hydroxy-MAB. N-Ethyl-4-aminoazobenzene, 4-aminoazobenzene, and their N-hydroxy derivatives did not induce significant numbers of tumors in any of the above assay systems. Administration to preweaning male mice of MAB, N-hydroxy-MAB, N-hydroxy-N-ethyl-4-aminoazobenzene, and N-hydroxy-4-aminoazobenzene resulted in high incidences and high multiplicities of hepatic tumors (averages of 5 to 7 tumors/mouse) within 1 year. N-Ethyl-4-aminoazobenzene and 4-aminoazobenzene also induced hepatic tumors under the same conditions, but they were less active. These data support the conclusion that the N-hydroxy metabolites of these aminoazo dyes are proximate carcinogens.", "contents": "The N-hydroxy metabolites of N-methyl-4-aminoazobenzene and related dyes as proximate carcinogens in the rat and mouse. The carcinogenicities for rats and mice of N-methyl-4-aminoazobenzene (MAB) and its hepatic microsomal metabolite N-hydroxy-N-methyl-4-aminoazobenzene (N-hydroxy-MAB) were compared under several conditions. N-Ethyl-4-aminoazobenzene, 4-aminoazobenzene, and their N-hydroxy derivatives were also included in some of the assays. About 25% of the rats given MAB or N-hydroxy-MAB (3 to 5 mmol/kg body weight) by stomach tube over a 5-week period developed hepatic tumors by 18 to 22 months. Similarly treated rats subsequently given phenobarbital in the drinking water until the termination of the experiment developed about twice as many hepatic tumors. N-Hydroxy-MAB, administered p.o., but not MAB, also induced multiple papillomas and extensive carcinomas of the forestomach in approximately 50% of the rats. Only low incidence of hepatocellular carcinomas occurred in partially hepatectomized rats given a single i.p. injection of 180 mumol/kg body weight of MAB or N-hydroxy-MAB with or without subsequent administration of phenobarbital. Although repeated s.c. doses of N-benzoyloxy-N-methyl-4-aminoazobenzene induced sarcomas at the injection site in 90% of the rats, only 3 of 20 rats developed sarcomas at the site of s.c. injections of N-hydroxy-MAB. N-Ethyl-4-aminoazobenzene, 4-aminoazobenzene, and their N-hydroxy derivatives did not induce significant numbers of tumors in any of the above assay systems. Administration to preweaning male mice of MAB, N-hydroxy-MAB, N-hydroxy-N-ethyl-4-aminoazobenzene, and N-hydroxy-4-aminoazobenzene resulted in high incidences and high multiplicities of hepatic tumors (averages of 5 to 7 tumors/mouse) within 1 year. N-Ethyl-4-aminoazobenzene and 4-aminoazobenzene also induced hepatic tumors under the same conditions, but they were less active. These data support the conclusion that the N-hydroxy metabolites of these aminoazo dyes are proximate carcinogens."}
{"id": "PMID:476672", "title": "Serine hydroxymethyltransferase activity and serine incorporation in leukocytes.", "content": "Studies of serine hydroxymethyltransferase activity in extracts of leukocytes from normal and leukemic subjects showed that the enzyme is present in lymphocytes and granulocytes but that activity is higher in lymphocytes. It is also higher than normal in lymphocytes from patients with chronic lymphocytic leukemia and to a lesser extent in the leukocytes of patients with acute myelocytic leukemia and acute lymphocytic leukemia. A striking increase in activity occurs in lymphocytes stimulated by phytohemagglutinin to divide in culture. Enzyme activity rises severalfold before cell number increases. Stimulated lymphocytes take up [3-14C]serine from the medium and incorporate its radioactivity into DNA, RNA, and other cell fractions. The rate of incorporation increases sharply before the rise in cell number. Thus, serine hydroxymethyltransferase activity and serine incorporation in vivo show a temporal correlation in stimulated lymphocytes. Inhibitors of DNA synthesis (e.g., fluorodeoxyuridine or high concentrations of adenosine or thymidine) block incorporation of serine radioactivity into DNA and other cell fractions. The results suggest that serine hydroxymethyltransferase activity and cellular uptake of serine have a significant role in proliferating cells.", "contents": "Serine hydroxymethyltransferase activity and serine incorporation in leukocytes. Studies of serine hydroxymethyltransferase activity in extracts of leukocytes from normal and leukemic subjects showed that the enzyme is present in lymphocytes and granulocytes but that activity is higher in lymphocytes. It is also higher than normal in lymphocytes from patients with chronic lymphocytic leukemia and to a lesser extent in the leukocytes of patients with acute myelocytic leukemia and acute lymphocytic leukemia. A striking increase in activity occurs in lymphocytes stimulated by phytohemagglutinin to divide in culture. Enzyme activity rises severalfold before cell number increases. Stimulated lymphocytes take up [3-14C]serine from the medium and incorporate its radioactivity into DNA, RNA, and other cell fractions. The rate of incorporation increases sharply before the rise in cell number. Thus, serine hydroxymethyltransferase activity and serine incorporation in vivo show a temporal correlation in stimulated lymphocytes. Inhibitors of DNA synthesis (e.g., fluorodeoxyuridine or high concentrations of adenosine or thymidine) block incorporation of serine radioactivity into DNA and other cell fractions. The results suggest that serine hydroxymethyltransferase activity and cellular uptake of serine have a significant role in proliferating cells."}
{"id": "PMID:476673", "title": "Enhancement of antitumor activity of 2,4-diamino-5-(3',4'-dichlorophenyl)-6-methylpyrimidine and Baker's antifol (triazinate) with carboxypeptidase G1.", "content": "Carboxypeptidase G1 (CPDG1), an enzyme that degrades folates but not the nonclassical folate antagonists triazinate (TZT, Baker's antifol) and 2,4-diamino-5-(3',4'-dichlorophenyl)-6-methylpyrimidine (DDMP), enhanced the antineoplastic activity of these antifolates. In 6-day cell culture experiments with Walker 256 carcinosarcoma, CPDG1 at levels up to 0.54 unit/ml showed very little inhibitory effect on growth. In the presence of 10(-7) M DDMP, the grown of Walker 256 cells was similar to that of controls, but in combination with CPDG1 (0.1 unit/ml) 80% growth inhibition was observed. TZT at a concentration of 10(-8) M did not affect the growth of Walker 256 cells. The combination of 10(-8) M TZT with CPDG1 (0.1 unit/ml), however, strongly inhibited cell growth. In experiments with rats bearing Walker 256 carcinosarcoma, the administration of CPDG1 (800 units/kg/day) from Day 1 to Day 6 resulted in no significant increase in life span. Administration of TZT in doses up to 0.05 mg/kg on Day 1 or that of DDMP up to 15 mg/kg on Days 1, 3, and 5 had no antitumor effects as measured by survival of the animals. However, CPDG1 (800 units/kg/day) from Day 1 to Day 6 in combination with TZT (0.05 mg/kg on Day 1) or DDMP (15 mg/kg on Days 1, 3, and 5) resulted in increases of 50 and 30%, respectively, in the survival of the tumor-bearing animals. These results demonstrate that CPDG1 enhances the antitumor effects of TZT or DDMP.", "contents": "Enhancement of antitumor activity of 2,4-diamino-5-(3',4'-dichlorophenyl)-6-methylpyrimidine and Baker's antifol (triazinate) with carboxypeptidase G1. Carboxypeptidase G1 (CPDG1), an enzyme that degrades folates but not the nonclassical folate antagonists triazinate (TZT, Baker's antifol) and 2,4-diamino-5-(3',4'-dichlorophenyl)-6-methylpyrimidine (DDMP), enhanced the antineoplastic activity of these antifolates. In 6-day cell culture experiments with Walker 256 carcinosarcoma, CPDG1 at levels up to 0.54 unit/ml showed very little inhibitory effect on growth. In the presence of 10(-7) M DDMP, the grown of Walker 256 cells was similar to that of controls, but in combination with CPDG1 (0.1 unit/ml) 80% growth inhibition was observed. TZT at a concentration of 10(-8) M did not affect the growth of Walker 256 cells. The combination of 10(-8) M TZT with CPDG1 (0.1 unit/ml), however, strongly inhibited cell growth. In experiments with rats bearing Walker 256 carcinosarcoma, the administration of CPDG1 (800 units/kg/day) from Day 1 to Day 6 resulted in no significant increase in life span. Administration of TZT in doses up to 0.05 mg/kg on Day 1 or that of DDMP up to 15 mg/kg on Days 1, 3, and 5 had no antitumor effects as measured by survival of the animals. However, CPDG1 (800 units/kg/day) from Day 1 to Day 6 in combination with TZT (0.05 mg/kg on Day 1) or DDMP (15 mg/kg on Days 1, 3, and 5) resulted in increases of 50 and 30%, respectively, in the survival of the tumor-bearing animals. These results demonstrate that CPDG1 enhances the antitumor effects of TZT or DDMP."}
{"id": "PMID:476674", "title": "An improved method for analyzing survival data from combination chemotherapy experiments.", "content": "In this paper, we present a new method for analyzing survival data from combination chemotherapy experiments. The analysis consists of relating survival to the dosage level of each drug in the combination and using response surface techniques to determine the importance of drug interactions and to estimate optimal doses. A combination experiment using cyclophosphamide, mechlorethamine, and mitomycin C in early L1210 leukemia, advanced L1210 leukemia, and advanced P388 leukemia is used to illustrate the analyses. A therapeutic synergism has been shown. As a result of the various drug interactions, the predicted optimal dose of mitomycin C is found to be zero. This result was duplicated in each tumor system studied.", "contents": "An improved method for analyzing survival data from combination chemotherapy experiments. In this paper, we present a new method for analyzing survival data from combination chemotherapy experiments. The analysis consists of relating survival to the dosage level of each drug in the combination and using response surface techniques to determine the importance of drug interactions and to estimate optimal doses. A combination experiment using cyclophosphamide, mechlorethamine, and mitomycin C in early L1210 leukemia, advanced L1210 leukemia, and advanced P388 leukemia is used to illustrate the analyses. A therapeutic synergism has been shown. As a result of the various drug interactions, the predicted optimal dose of mitomycin C is found to be zero. This result was duplicated in each tumor system studied."}
{"id": "PMID:476675", "title": "Enhancement of the thermal response of animal tumors by Corynebacterium parvum.", "content": "The effect of Corynecbacterium parvum treatment on the thermal response of animal tumors was studied. Tumors were methylcholanthrene-induced (FSa-II) and spontaneous (FSa-I) fibrosarcomas in C3Hf/Sed mice. C. parvum was given i.v. and was followed by local hyperthermia at 43.5 degrees 3 days later. Cell survival determined by lung colony assays showed that preadministration of C. parvum insignificantly enhanced the thermal response of both tumors. Studies of delay of tumor growth for FSa-II demonstrated that the enhancement ratio decreased with increasing time of treatment and reached a minimum of approximately equal to 1.7. The enhancement ratio for the time at hyperthermia which achieved tumor control in one-half of the treated tumors was 1.7. Together with our previous results on normal tissue responses, the therapeutic gain factor for obtaining 50% tumor control was found to be 1.1 (1.7/1.55) for the weekly immunogenic FSa-II tumor, while it was 2.3 for moderately immunogenic FSa-I as reported previously.", "contents": "Enhancement of the thermal response of animal tumors by Corynebacterium parvum. The effect of Corynecbacterium parvum treatment on the thermal response of animal tumors was studied. Tumors were methylcholanthrene-induced (FSa-II) and spontaneous (FSa-I) fibrosarcomas in C3Hf/Sed mice. C. parvum was given i.v. and was followed by local hyperthermia at 43.5 degrees 3 days later. Cell survival determined by lung colony assays showed that preadministration of C. parvum insignificantly enhanced the thermal response of both tumors. Studies of delay of tumor growth for FSa-II demonstrated that the enhancement ratio decreased with increasing time of treatment and reached a minimum of approximately equal to 1.7. The enhancement ratio for the time at hyperthermia which achieved tumor control in one-half of the treated tumors was 1.7. Together with our previous results on normal tissue responses, the therapeutic gain factor for obtaining 50% tumor control was found to be 1.1 (1.7/1.55) for the weekly immunogenic FSa-II tumor, while it was 2.3 for moderately immunogenic FSa-I as reported previously."}
{"id": "PMID:476677", "title": "Enhancing effect of thyroxine on tumor growth and metastases in syngeneic mouse tumor systems.", "content": "We studied the effect of thyroxine treatment on tumor growth and metastases resulting from tumor implants on the hind feet of mice in two syngeneic systems. In control, untreated A/Jax mice, tumor Sarcoma 1 at Day 14 after implantation had average tumor weight of 582 +/- 60 (S.D.) mg and showed an incidence of 57% metastases to regional popliteal nodes and 5% metastases to thymus. In contrast, the thyroxine-treated group (40 microgram/mouse s.c., 5 times/week for 1 month) had an average tumor weight of 808 +/- 56 mg (p less than 0.001), and metastases to popliteal nodes and thymus were 90 and 35%, respectively. In another syngeneic tumor system, Lewis fibrosarcoma was implanted in C57BL/6J mice, and the tumor weight and metastatic index (derived from the number and size of the pulmonary tumor foci) were determined at Day 28. Again, the synthetic L-thyroxine treated group showed a significant enhancement tumor growth and metastatic index. The mean tumor weight in the treated group was 385 +/- 26 mg (control, 694 +/- 25 mg; p less than 0.005) and metastatic index was 84 +/- 29 (control, 30 +/- 25; p less than 0.001). Induced hypothyroidism (treatment with 131I, 100 microCi/mouse i.p.) showed the reverse effect on both tumor systems. These results suggest that both tumor systems are dependent on thyroid hormones for their growth and spread.", "contents": "Enhancing effect of thyroxine on tumor growth and metastases in syngeneic mouse tumor systems. We studied the effect of thyroxine treatment on tumor growth and metastases resulting from tumor implants on the hind feet of mice in two syngeneic systems. In control, untreated A/Jax mice, tumor Sarcoma 1 at Day 14 after implantation had average tumor weight of 582 +/- 60 (S.D.) mg and showed an incidence of 57% metastases to regional popliteal nodes and 5% metastases to thymus. In contrast, the thyroxine-treated group (40 microgram/mouse s.c., 5 times/week for 1 month) had an average tumor weight of 808 +/- 56 mg (p less than 0.001), and metastases to popliteal nodes and thymus were 90 and 35%, respectively. In another syngeneic tumor system, Lewis fibrosarcoma was implanted in C57BL/6J mice, and the tumor weight and metastatic index (derived from the number and size of the pulmonary tumor foci) were determined at Day 28. Again, the synthetic L-thyroxine treated group showed a significant enhancement tumor growth and metastatic index. The mean tumor weight in the treated group was 385 +/- 26 mg (control, 694 +/- 25 mg; p less than 0.005) and metastatic index was 84 +/- 29 (control, 30 +/- 25; p less than 0.001). Induced hypothyroidism (treatment with 131I, 100 microCi/mouse i.p.) showed the reverse effect on both tumor systems. These results suggest that both tumor systems are dependent on thyroid hormones for their growth and spread."}
{"id": "PMID:476678", "title": "Mutagen-induced disturbances in the DNA of human lymphocytes detected by antinucleoside antibodies.", "content": "The alkylating mutagens N-methyl-N'-nitro-N-nitrosoguanidine, methyl methanesulfonate, and N-nitroso-methylurea induced immunoreactivity to antinucleoside antibodies in human peripheral blood lymphocytes in vitro. This could also be detected in lymphocytes taken from a patient soon after i.v. administration of cyclophosphamide. The immunoreactivity response, which indicates denatured DNA or DNA single-strand breaks, was scored by immunofluorescent or immunoperoxidase techniques. Examination of blood from 10 normal subjects showed that 32 +/- 4% (S.E.) of resting peripheral blood lymphocytes were immunoreactive to antinucleoside antibodies. We have shown that these naturally occurring immunoreactive lymphocytes are largely accounted for by a subpopulation of thymus-derived lymphocytes bearing the Fc receptor for immunoglobulin M. The presence of these cells did not interfere with the use of peripheral blood lymphocytes for in vitro measurement of additional immunoreactivity caused by alkylating mutagens. The response proved to be dose dependent; up to 90% of lymphocytes could be rendered immunoreactive. Parallel studies with HeLa cells showed a similar dose-response relationship between mutagen action and immunoreactivity. With some agents, the immunoreactivity technique detected effects at lower concentrations than could be detected by HeLa cell survival studies. With N-nitrosomethylurea, measurement of DNA repair synthesis by [3H]thymidine autoradiography showed that in HeLa cells these two parameters of response to DNA damage increased in parallel. Our results provide a new basis for detecting the action of alkylating mutagens on human lymphocytes in vitro or in vivo.", "contents": "Mutagen-induced disturbances in the DNA of human lymphocytes detected by antinucleoside antibodies. The alkylating mutagens N-methyl-N'-nitro-N-nitrosoguanidine, methyl methanesulfonate, and N-nitroso-methylurea induced immunoreactivity to antinucleoside antibodies in human peripheral blood lymphocytes in vitro. This could also be detected in lymphocytes taken from a patient soon after i.v. administration of cyclophosphamide. The immunoreactivity response, which indicates denatured DNA or DNA single-strand breaks, was scored by immunofluorescent or immunoperoxidase techniques. Examination of blood from 10 normal subjects showed that 32 +/- 4% (S.E.) of resting peripheral blood lymphocytes were immunoreactive to antinucleoside antibodies. We have shown that these naturally occurring immunoreactive lymphocytes are largely accounted for by a subpopulation of thymus-derived lymphocytes bearing the Fc receptor for immunoglobulin M. The presence of these cells did not interfere with the use of peripheral blood lymphocytes for in vitro measurement of additional immunoreactivity caused by alkylating mutagens. The response proved to be dose dependent; up to 90% of lymphocytes could be rendered immunoreactive. Parallel studies with HeLa cells showed a similar dose-response relationship between mutagen action and immunoreactivity. With some agents, the immunoreactivity technique detected effects at lower concentrations than could be detected by HeLa cell survival studies. With N-nitrosomethylurea, measurement of DNA repair synthesis by [3H]thymidine autoradiography showed that in HeLa cells these two parameters of response to DNA damage increased in parallel. Our results provide a new basis for detecting the action of alkylating mutagens on human lymphocytes in vitro or in vivo."}
{"id": "PMID:476679", "title": "Evaluation of ribonucleoside and deoxyribonucleoside triphosphate pools in cultured leukemia cells during exposure to methotrexate or methotrexate plus thymidine.", "content": "Continuous exposure to inhibitory concentrations of methotrexate produces distinct rates of steady-state growth of murine leukemia L1210 and human leukemia CCRF-CEM cells in culture. Addition of thymidine to the medium produces reversal (6 to 40%) of this steady-state growth rate inhibition. This study utilized combinations of methotrexate and thymidine for an evaluation of the accompanying relationship between steady-state growth rate and changes in the ribo- and deoxyribonucleoside triphosphate pools. In L1210 cells exposed to methotrexate alone, the deoxythymidine 5'-phosphate (dTTP) pools decreased, whereas deoxyadenosine 5'-triphosphate, deoxyguanosine 5'-triphosphate, and deoxycytidine 5'-triphosphate (dCTP) remained relatively constant up to 70% inhibition of growth rate, with dCTP at a constant 112% of controls. The corresponding ribonucleoside triphosphates decreased only slightly. With the combination of methotrexate and thymidine resulting in up to 40% inhibition of growth rate, there was also a decrease in the dTTP pool while the other deoxyribonucleoside triphosphates remained relatively constant, and the corresponding ribonucleoside triphosphates again decreased only slightly. The dCTP pool was reduced to a constant 42% of control comparable to that produced by thymidine alone. With greater than 40% (with thymidine) or 70% (without thymidine) inhibition of growth rate, all pools decreased, but only dTTP was substantially reduced in proportion to the growth rate inhibition caused by methotrexate. The dTTP pool became depleted in spite of the presence of exogenous thymidine. Evaluation of CCRF-CEM cells indicated that inhibition of growth rate and nucleotide pool perturbations by methotrexate were similar to those observed in L1210 cells. However, in the presence of thymidine, inhibition of growth rate appeared related to decreased pools of dCTP, deoxyadenosine 5'-triphosphate, and deoxyguanosine 5'-triphosphate, rather than dTTP as was observed for L1210 cells. Hence, mammalian cells were capable of responding in a differential fashion to pharmacological perturbations, and this capacity may play a role in determining therapeutic selectivity. Since the ribonucleoside triphosphate decreases were slight and relatively uniform during methotrexate-induced perturbations, the deoxyribonucleoside triphosphate pools appear to be more directly related to inhibition of growth rate. The results are consistent with the concept that slight imbalances in the deoxyribonucleoside triphosphate pools dramatically inhibit DNA synthesis, as mediated through their interaction with DNA polymerase.", "contents": "Evaluation of ribonucleoside and deoxyribonucleoside triphosphate pools in cultured leukemia cells during exposure to methotrexate or methotrexate plus thymidine. Continuous exposure to inhibitory concentrations of methotrexate produces distinct rates of steady-state growth of murine leukemia L1210 and human leukemia CCRF-CEM cells in culture. Addition of thymidine to the medium produces reversal (6 to 40%) of this steady-state growth rate inhibition. This study utilized combinations of methotrexate and thymidine for an evaluation of the accompanying relationship between steady-state growth rate and changes in the ribo- and deoxyribonucleoside triphosphate pools. In L1210 cells exposed to methotrexate alone, the deoxythymidine 5'-phosphate (dTTP) pools decreased, whereas deoxyadenosine 5'-triphosphate, deoxyguanosine 5'-triphosphate, and deoxycytidine 5'-triphosphate (dCTP) remained relatively constant up to 70% inhibition of growth rate, with dCTP at a constant 112% of controls. The corresponding ribonucleoside triphosphates decreased only slightly. With the combination of methotrexate and thymidine resulting in up to 40% inhibition of growth rate, there was also a decrease in the dTTP pool while the other deoxyribonucleoside triphosphates remained relatively constant, and the corresponding ribonucleoside triphosphates again decreased only slightly. The dCTP pool was reduced to a constant 42% of control comparable to that produced by thymidine alone. With greater than 40% (with thymidine) or 70% (without thymidine) inhibition of growth rate, all pools decreased, but only dTTP was substantially reduced in proportion to the growth rate inhibition caused by methotrexate. The dTTP pool became depleted in spite of the presence of exogenous thymidine. Evaluation of CCRF-CEM cells indicated that inhibition of growth rate and nucleotide pool perturbations by methotrexate were similar to those observed in L1210 cells. However, in the presence of thymidine, inhibition of growth rate appeared related to decreased pools of dCTP, deoxyadenosine 5'-triphosphate, and deoxyguanosine 5'-triphosphate, rather than dTTP as was observed for L1210 cells. Hence, mammalian cells were capable of responding in a differential fashion to pharmacological perturbations, and this capacity may play a role in determining therapeutic selectivity. Since the ribonucleoside triphosphate decreases were slight and relatively uniform during methotrexate-induced perturbations, the deoxyribonucleoside triphosphate pools appear to be more directly related to inhibition of growth rate. The results are consistent with the concept that slight imbalances in the deoxyribonucleoside triphosphate pools dramatically inhibit DNA synthesis, as mediated through their interaction with DNA polymerase."}
{"id": "PMID:476682", "title": "Metabolic conversion of indicine N-oxide to indicine in rabbits and humans.", "content": "Indicine N-oxide, a pyrrolizidine alkaloid N-oxide that exhibits antitumor activity without some of the toxic effects associated with other pyrrolizidine alkaloids, is metabolized to indicine in rabbits and humans. Indicine can be detected in the plasma and is excreted in the urine in a dose-dependent manner following the i.v. administration of indicine N-oxide. The p.o. administration of indicine N-oxide leads to an increased plasma concentration and an increased urinary excretion of indicine. The hepatic microsomal fraction and the gut flora both catalyze the anaerobic reduction of indicine N-oxide to indicine in vitro. Whole-animal studies suggest that the gut flora play a major role in the metabolic reduction of indicine N-oxide by the rabbit. Indicine N-oxide is not actively excreted in the bile, and it is probable that indicine N-oxide finds its way into the gut by passive diffusion following i.v. administration. Neomycin and erythromycin, which reduce the number of anaerobic bacteria in the gut, lead to decreased plasma levels and a decreased urinary excretion of indicine.", "contents": "Metabolic conversion of indicine N-oxide to indicine in rabbits and humans. Indicine N-oxide, a pyrrolizidine alkaloid N-oxide that exhibits antitumor activity without some of the toxic effects associated with other pyrrolizidine alkaloids, is metabolized to indicine in rabbits and humans. Indicine can be detected in the plasma and is excreted in the urine in a dose-dependent manner following the i.v. administration of indicine N-oxide. The p.o. administration of indicine N-oxide leads to an increased plasma concentration and an increased urinary excretion of indicine. The hepatic microsomal fraction and the gut flora both catalyze the anaerobic reduction of indicine N-oxide to indicine in vitro. Whole-animal studies suggest that the gut flora play a major role in the metabolic reduction of indicine N-oxide by the rabbit. Indicine N-oxide is not actively excreted in the bile, and it is probable that indicine N-oxide finds its way into the gut by passive diffusion following i.v. administration. Neomycin and erythromycin, which reduce the number of anaerobic bacteria in the gut, lead to decreased plasma levels and a decreased urinary excretion of indicine."}
{"id": "PMID:476683", "title": "Cytotoxic effects of chemotherapeutic drugs on mouse testis cells.", "content": "Studies of testicular cell killing in mice by several chemotherapeutic drugs have been performed to EVALUATE THE HARMFUL EFFECTS OF ONCOLYTIC AGENTS ON REPRODUCTION. Seven drugs, Adriamycin, 1-beta-D-arabinofuranosylcytosine, bleomycin, cyclophosphamide, hydroxyurea, vinblastine, and vincristine, given as single injections, were cytotoxic to differentiated spermatogonia. Adriamycin was also highly effective in killing stem cells. The other drugs produced little or no stem cell loss even at doses toxic to the animals. Negligible killing of spermatocytes and spermatids was noted at any dose level. The results demonstrated that oncolytic agents preferntially killed cells at specific stages of the spermatogenic pathway in mice at doses within the clinical range for humans.", "contents": "Cytotoxic effects of chemotherapeutic drugs on mouse testis cells. Studies of testicular cell killing in mice by several chemotherapeutic drugs have been performed to EVALUATE THE HARMFUL EFFECTS OF ONCOLYTIC AGENTS ON REPRODUCTION. Seven drugs, Adriamycin, 1-beta-D-arabinofuranosylcytosine, bleomycin, cyclophosphamide, hydroxyurea, vinblastine, and vincristine, given as single injections, were cytotoxic to differentiated spermatogonia. Adriamycin was also highly effective in killing stem cells. The other drugs produced little or no stem cell loss even at doses toxic to the animals. Negligible killing of spermatocytes and spermatids was noted at any dose level. The results demonstrated that oncolytic agents preferntially killed cells at specific stages of the spermatogenic pathway in mice at doses within the clinical range for humans."}
{"id": "PMID:476684", "title": "Induction of sarcomas in nude mice by implantation of Syrian hamster fetal cells exposed in vitro to nickel subsulfide.", "content": "In vitro exposure of Syrian hamster fetal cells to nickel subsulfide (alpha Ni3S2) yielded positive colony assays for morphological transformation. A dose-response relationship was found between the concentration of alpha Ni3S2 and the incidence of morphological transformation. Exposures of alpha Ni3S2 induced morphological transformation at concentrations (0.1 or 1.0 microgram/ml of culture medium) which did not impair cell plating efficiency. Nickel monosulfide (NiS) did not induce morphological transformation of Syrian hamster fetal cells under the same conditions. Clones of alpha Ni3S2-transformed cells were able to grow in soft agar medium and demonstrated increased basal and induced activities of ornithine decarboxylase. Undifferentiated sarcomas developed in 26 of 27 nude mice at the site of s.c. injection of clones of alpha Ni3S2-transformed cells. No tumors developed in 19 control nude mice which were given s.c. injections of nontransformed Syrian hamster fetal cells which had not been exposed to alpha Ni3S2. This study demonstrates that fetal cells which undergo transformation following exposure to alpha Ni3S2 are capable of producing malignant tumors in nude mice.", "contents": "Induction of sarcomas in nude mice by implantation of Syrian hamster fetal cells exposed in vitro to nickel subsulfide. In vitro exposure of Syrian hamster fetal cells to nickel subsulfide (alpha Ni3S2) yielded positive colony assays for morphological transformation. A dose-response relationship was found between the concentration of alpha Ni3S2 and the incidence of morphological transformation. Exposures of alpha Ni3S2 induced morphological transformation at concentrations (0.1 or 1.0 microgram/ml of culture medium) which did not impair cell plating efficiency. Nickel monosulfide (NiS) did not induce morphological transformation of Syrian hamster fetal cells under the same conditions. Clones of alpha Ni3S2-transformed cells were able to grow in soft agar medium and demonstrated increased basal and induced activities of ornithine decarboxylase. Undifferentiated sarcomas developed in 26 of 27 nude mice at the site of s.c. injection of clones of alpha Ni3S2-transformed cells. No tumors developed in 19 control nude mice which were given s.c. injections of nontransformed Syrian hamster fetal cells which had not been exposed to alpha Ni3S2. This study demonstrates that fetal cells which undergo transformation following exposure to alpha Ni3S2 are capable of producing malignant tumors in nude mice."}
{"id": "PMID:476685", "title": "Isolation and characterization of alpha-lactalbumin from a rat mammary tumor.", "content": "alpha-Lactalbumin was purified to homogeneity from the R3230AC rat mammary adenocarcinoma. alpha-Lactalbumin from the R3230AC tumor had the same amino acid and carbohydrate composition, migration on gel electrophoresis, and specific activity as did alpha-lactalbumin isolated from normal rat milk.", "contents": "Isolation and characterization of alpha-lactalbumin from a rat mammary tumor. alpha-Lactalbumin was purified to homogeneity from the R3230AC rat mammary adenocarcinoma. alpha-Lactalbumin from the R3230AC tumor had the same amino acid and carbohydrate composition, migration on gel electrophoresis, and specific activity as did alpha-lactalbumin isolated from normal rat milk."}
{"id": "PMID:476687", "title": "Protection of mice against lethal dosages of nebularine by nitrobenzylthioinosine, an inhibitor of nucleoside transport.", "content": "In the presence of nitrobenzylthioinosine (NBMPR) a potent inhibitor of nucleoside transport, Roswell Park Memorial Institute 6410 cells proliferating in culture were protected from otherwise inhibitory concentrations of 9-beta-D-ribofuranosylpurine (nebularine); cellular uptake of nebularine was greatly reduced under these circumstances. Initial rates of nebularine uptake by Roswell Park Memorial Institute 6410 cells were inhibited by NBMPR, indicating that the latter interfered with nebularine transport. NBMPR protected mice against potentially lethal treatment regimens with nebularine, 4-amino-7-(beta-D-ribofuranosyl)pyrrolo[2,3-d]pyrimidine (tubercidin) or 4-amino-5-cyano-7-(beta-D-ribofuranosyl)pyrrolo[2,3-d]pyrimidine (toyocamycin); protection resulted when NBMPR was administered i.p. in advance of or simultaneously with nebularine, but not when NBMPR followed nebularine by 1 hr. Both NBMPR and its 5'-monophosphate protected mice against nebularine lethality when administered s.c.", "contents": "Protection of mice against lethal dosages of nebularine by nitrobenzylthioinosine, an inhibitor of nucleoside transport. In the presence of nitrobenzylthioinosine (NBMPR) a potent inhibitor of nucleoside transport, Roswell Park Memorial Institute 6410 cells proliferating in culture were protected from otherwise inhibitory concentrations of 9-beta-D-ribofuranosylpurine (nebularine); cellular uptake of nebularine was greatly reduced under these circumstances. Initial rates of nebularine uptake by Roswell Park Memorial Institute 6410 cells were inhibited by NBMPR, indicating that the latter interfered with nebularine transport. NBMPR protected mice against potentially lethal treatment regimens with nebularine, 4-amino-7-(beta-D-ribofuranosyl)pyrrolo[2,3-d]pyrimidine (tubercidin) or 4-amino-5-cyano-7-(beta-D-ribofuranosyl)pyrrolo[2,3-d]pyrimidine (toyocamycin); protection resulted when NBMPR was administered i.p. in advance of or simultaneously with nebularine, but not when NBMPR followed nebularine by 1 hr. Both NBMPR and its 5'-monophosphate protected mice against nebularine lethality when administered s.c."}
{"id": "PMID:476689", "title": "Carcinogenesis in rat esophagus by intraperitoneal injection of different doses of methyl-n-amylnitrosamine.", "content": "The carcinogenicity of methyl-n-amylnitrosamine in MRC-Wistar rats was determined after i.p. injection at a variety of dose schedules. After 6 weekly methyl-n-amylnitrosamine injections of 25 mg/kg or 12 weekly injections of either 12.5 or 25 mg/kg, the incidence of esophageal squamous cell papillomas was 85 to 100% and that of esophageal squamous cell carcinomas was 40 to 65%. With 12 injections, the mean survival time was 25 to 31 weeks. Treatment with 1 or 2 doses of 50 mg/kg produced a lesser incidence (less than 20%) of esophageal tumors, with a longer survival time of 67 to 77 weeks. One 85-mg/kg injection caused esophageal carcinomas in 5 of 7 rats. The treated groups also had squamous cell papillomas and carcinomas in the nasal cavity (up to 50% incidence) and trachea (up to 30% incidence). Hence, a 6- or 12-week treatment schedule was adequate for inducing esophageal tumors and could be used for studies on agents modifying esophageal tumor induction by methyl-n-amylnitrosamine.", "contents": "Carcinogenesis in rat esophagus by intraperitoneal injection of different doses of methyl-n-amylnitrosamine. The carcinogenicity of methyl-n-amylnitrosamine in MRC-Wistar rats was determined after i.p. injection at a variety of dose schedules. After 6 weekly methyl-n-amylnitrosamine injections of 25 mg/kg or 12 weekly injections of either 12.5 or 25 mg/kg, the incidence of esophageal squamous cell papillomas was 85 to 100% and that of esophageal squamous cell carcinomas was 40 to 65%. With 12 injections, the mean survival time was 25 to 31 weeks. Treatment with 1 or 2 doses of 50 mg/kg produced a lesser incidence (less than 20%) of esophageal tumors, with a longer survival time of 67 to 77 weeks. One 85-mg/kg injection caused esophageal carcinomas in 5 of 7 rats. The treated groups also had squamous cell papillomas and carcinomas in the nasal cavity (up to 50% incidence) and trachea (up to 30% incidence). Hence, a 6- or 12-week treatment schedule was adequate for inducing esophageal tumors and could be used for studies on agents modifying esophageal tumor induction by methyl-n-amylnitrosamine."}
{"id": "PMID:476691", "title": "Quantitative models for growth inhibition of human leukemia cells by antitumor anthracycline derivatives.", "content": "A batch elution method with hydroxylapatite was developed to assay DNA damage by a set of antitumor anthracycline derivatives and was standardized with respect to the kinetics of unwinding, size of the alkaline unwinding unit, and fidelity of selective elution of single and double-stranded DNA. The method was applied to a study of a set of 10 antitumor anthracycline derivatives which inhibit growth of CCRF-CEM human leukemia cells over a range of potencies exceeding four orders of magnitude. The derivatives, including Adriamycin, daunorubicin, and carminomycin, vary in structure at C-4 and C-13, with substitutions at C-14 and N and stereochemical differences at C-4'. In a static model (fixed drug concentrations and incubation times), the potency [1/ID37 (concentration of agent that inhibits cell growth by 37%)] of nine of the ten derivatives may be expressed as functions of DNA damage (n), inhibition of thymidine incorporation (l), and drug retention (r): ID37 = Ka(r/l . n)kb, with a coefficient of correlation of greater than 0.99. A kinetic model with 4-demethoxydaunorubicin (varying concentrations and incubation times) was also described. Following initial uptake and a period of rapid loss after cells are washed free of excess drug, the change in agent concentration in the cells follows first-order kinetics. The cell index (cell number after 50 hr in drug-free growth medium/cell number after initial 2-hr exposure with drug) may be expressed linearly in terms of the kinetics of drug loss (coefficient of correlation, greater than 0.98), or as functions of 1/n (coefficient of correlation, greater than 0.958), 1/l . n (coefficient of correlation, greater than 0.963, or r/l . n (coefficient of correlation, greater than 0.963). These studies may be used to define a class of similarly acting anthracycline agents and to give some insight into the mechanism of action of the agents that fall within the class.", "contents": "Quantitative models for growth inhibition of human leukemia cells by antitumor anthracycline derivatives. A batch elution method with hydroxylapatite was developed to assay DNA damage by a set of antitumor anthracycline derivatives and was standardized with respect to the kinetics of unwinding, size of the alkaline unwinding unit, and fidelity of selective elution of single and double-stranded DNA. The method was applied to a study of a set of 10 antitumor anthracycline derivatives which inhibit growth of CCRF-CEM human leukemia cells over a range of potencies exceeding four orders of magnitude. The derivatives, including Adriamycin, daunorubicin, and carminomycin, vary in structure at C-4 and C-13, with substitutions at C-14 and N and stereochemical differences at C-4'. In a static model (fixed drug concentrations and incubation times), the potency [1/ID37 (concentration of agent that inhibits cell growth by 37%)] of nine of the ten derivatives may be expressed as functions of DNA damage (n), inhibition of thymidine incorporation (l), and drug retention (r): ID37 = Ka(r/l . n)kb, with a coefficient of correlation of greater than 0.99. A kinetic model with 4-demethoxydaunorubicin (varying concentrations and incubation times) was also described. Following initial uptake and a period of rapid loss after cells are washed free of excess drug, the change in agent concentration in the cells follows first-order kinetics. The cell index (cell number after 50 hr in drug-free growth medium/cell number after initial 2-hr exposure with drug) may be expressed linearly in terms of the kinetics of drug loss (coefficient of correlation, greater than 0.98), or as functions of 1/n (coefficient of correlation, greater than 0.958), 1/l . n (coefficient of correlation, greater than 0.963, or r/l . n (coefficient of correlation, greater than 0.963). These studies may be used to define a class of similarly acting anthracycline agents and to give some insight into the mechanism of action of the agents that fall within the class."}
{"id": "PMID:476693", "title": "Ultrastructure of doxorubicin (adriamycin)-induced skin ulcers in rats.", "content": "Skin necrosis was produced in 24 male Fischer 344 rats by intradermal injection of 0.5 ml of doxorubicin (Adriamycin) at a concentration of 2 mg/ml. The resulting wounds healed slowly over 6 to 7 weeks with the reduced contraction rate paralleling the prolonged morbidity of doxorubicin ulcers in humans. Electron microscopy showed bizarre rough endoplasmic reticulum, double-walled  vacuoles, and swollen mitochondria from 1 through 12 weeks after injury. Myofibroblasts with 60- to 80-A microfilaments with electron-dense bodies, intercellular connections, and prominent microtubules were seen from 4 through 12 weeks after injury. Although the appearance of myofibroblasts was delayed, their structure was normal. The delayed contraction of doxorubicin-induced skin ulcers thus appears due to persistent nonspecific cellular damage at the nuclear level rather than to specific derangement of myofibroblast function.", "contents": "Ultrastructure of doxorubicin (adriamycin)-induced skin ulcers in rats. Skin necrosis was produced in 24 male Fischer 344 rats by intradermal injection of 0.5 ml of doxorubicin (Adriamycin) at a concentration of 2 mg/ml. The resulting wounds healed slowly over 6 to 7 weeks with the reduced contraction rate paralleling the prolonged morbidity of doxorubicin ulcers in humans. Electron microscopy showed bizarre rough endoplasmic reticulum, double-walled  vacuoles, and swollen mitochondria from 1 through 12 weeks after injury. Myofibroblasts with 60- to 80-A microfilaments with electron-dense bodies, intercellular connections, and prominent microtubules were seen from 4 through 12 weeks after injury. Although the appearance of myofibroblasts was delayed, their structure was normal. The delayed contraction of doxorubicin-induced skin ulcers thus appears due to persistent nonspecific cellular damage at the nuclear level rather than to specific derangement of myofibroblast function."}
{"id": "PMID:476696", "title": "Photodynamic inactivation of synchronized human cells in vitro in the presence of hematoporphyrin.", "content": "Dose-response curves for inactivation of synchronized cells from the human cell line NHK 3025 in the presence of hematoporphyrin and light are shown. Sensitivity toward photodynamic inactivation is increasing as the cells move in the cell cycle from G1 to mid-S. The increase is mostly due to a change in the extent of the shoulder of the dose-response curve at low doses. The quasithreshold dose (Dq) is high in early G1 and much lower in mid-S. No great variations were found in the slope (1/Do) of the dose-response curves at high doses. The shape of the dose-response curves indicates a higher capacity for repair of sublethal damage in the early part of the cell cycle than in the later stages.", "contents": "Photodynamic inactivation of synchronized human cells in vitro in the presence of hematoporphyrin. Dose-response curves for inactivation of synchronized cells from the human cell line NHK 3025 in the presence of hematoporphyrin and light are shown. Sensitivity toward photodynamic inactivation is increasing as the cells move in the cell cycle from G1 to mid-S. The increase is mostly due to a change in the extent of the shoulder of the dose-response curve at low doses. The quasithreshold dose (Dq) is high in early G1 and much lower in mid-S. No great variations were found in the slope (1/Do) of the dose-response curves at high doses. The shape of the dose-response curves indicates a higher capacity for repair of sublethal damage in the early part of the cell cycle than in the later stages."}
{"id": "PMID:476697", "title": "Effects of daunorubicin and doxorubicin, free and associated with DNA, on hemopoietic stem cells.", "content": "We have compared daunorubicin (DNR)-DNA with free DNR and doxorubicin (DOX)-DNA with free DOX for their effects in vivo in mice on pluripotent stem cells and granulocytic committed stem cells. Dose-survival, time-survival, and recovery curves were obtained after one i.v. injection of either drug. The dose-survival curves of colony-forming units-spleen (CFU-S) and colony-forming units-committed stem cells (CFU-C) were exponential in shape with both agents. DNR-DNA appeared more toxic to the hemopoietic precursor cells than did free DNR. In contrast, DOX-DNA was less toxic toward CFU-S and as toxic as DOX toward CFU-C. Time-survival curves indicated a minimum level of CFU-S and CFU-C at about 33 hr. After that, the recovery of CFU-S was rapid for DNR-treated mice but remained below 50% of the controls on Day 12 for the DNR-DNA-treated group. In mice previously given injections of DOX or DOX-DNA, the recovery of the CFU-S was more protracted in time with a better recovery in mice treated with DOX-DNA. Both DNR and DNR-DNA induced an initial CFU-C decrease followed by a rapid but transient rise with a maximum on Day 4 after chemotherapy. On Day 12, the CFU-C recovery was still incomplete in both DNR- and DNR-DNA-treated mice. In the groups treated with DOX, the CFU-C recovery was more important after DOX-DNA complex than after free DOX. The results are discussed in view of the \"lysosomotropic chemotherapy\" hypothesis.", "contents": "Effects of daunorubicin and doxorubicin, free and associated with DNA, on hemopoietic stem cells. We have compared daunorubicin (DNR)-DNA with free DNR and doxorubicin (DOX)-DNA with free DOX for their effects in vivo in mice on pluripotent stem cells and granulocytic committed stem cells. Dose-survival, time-survival, and recovery curves were obtained after one i.v. injection of either drug. The dose-survival curves of colony-forming units-spleen (CFU-S) and colony-forming units-committed stem cells (CFU-C) were exponential in shape with both agents. DNR-DNA appeared more toxic to the hemopoietic precursor cells than did free DNR. In contrast, DOX-DNA was less toxic toward CFU-S and as toxic as DOX toward CFU-C. Time-survival curves indicated a minimum level of CFU-S and CFU-C at about 33 hr. After that, the recovery of CFU-S was rapid for DNR-treated mice but remained below 50% of the controls on Day 12 for the DNR-DNA-treated group. In mice previously given injections of DOX or DOX-DNA, the recovery of the CFU-S was more protracted in time with a better recovery in mice treated with DOX-DNA. Both DNR and DNR-DNA induced an initial CFU-C decrease followed by a rapid but transient rise with a maximum on Day 4 after chemotherapy. On Day 12, the CFU-C recovery was still incomplete in both DNR- and DNR-DNA-treated mice. In the groups treated with DOX, the CFU-C recovery was more important after DOX-DNA complex than after free DOX. The results are discussed in view of the \"lysosomotropic chemotherapy\" hypothesis."}
{"id": "PMID:476698", "title": "Quantitation of intracellular metabolites of [35S]-6-mercaptopurine in L5178Y cells grown in time-course incubates.", "content": "6-Mercaptopurine (6MP) metabolism was quantitatively determined in L5178Y murine lymphoma. Cells grown in time-course incubates with [35S]-6MP were extracted with cold perchloric acid, and the buffered extracts were subjected to high-performance liquid cation-exchange chromatography prior to and after hydrolysis with alkaline phosphatase. Free sulfate, 6-thiouric acid, 6-thioxanthosine, 6-thioguanosine, 6-thioinosine, free 6MP, and 6-methylthioinosine were separated from each other; identified in the radiochromatograms by elution volume, UV spectroscopic data, and enzymatic peak-shifting analyses with purine nucleoside phosphorylase; and quantitatively determined by means of 35S radioactivity. Gross intracellular 35S concentrations remained constant at 5 x 10(-5) M after 1 hr of incubation. 6MP metabolism in L5178Y cells was distinguished into an early phase (to 1 hr of incubation) in which 6MP was predominantly catabolized to 6-thiouric acid and free sulfate, into an intermediate phase (to 8 hr) in which substantial amounts of free 6MP and of ribonucleotides of 6-thioxanthosine and 6-thioguanosine were present while the concentrations of nonnucleotide oxidation products sharply decreased, and into a late phase (to 24 hr) in which the ribonucleotides of 6MP, of 6-thioguanosine and, in particular, of 6-methylthioinosine were the most abundant metabolites.", "contents": "Quantitation of intracellular metabolites of [35S]-6-mercaptopurine in L5178Y cells grown in time-course incubates. 6-Mercaptopurine (6MP) metabolism was quantitatively determined in L5178Y murine lymphoma. Cells grown in time-course incubates with [35S]-6MP were extracted with cold perchloric acid, and the buffered extracts were subjected to high-performance liquid cation-exchange chromatography prior to and after hydrolysis with alkaline phosphatase. Free sulfate, 6-thiouric acid, 6-thioxanthosine, 6-thioguanosine, 6-thioinosine, free 6MP, and 6-methylthioinosine were separated from each other; identified in the radiochromatograms by elution volume, UV spectroscopic data, and enzymatic peak-shifting analyses with purine nucleoside phosphorylase; and quantitatively determined by means of 35S radioactivity. Gross intracellular 35S concentrations remained constant at 5 x 10(-5) M after 1 hr of incubation. 6MP metabolism in L5178Y cells was distinguished into an early phase (to 1 hr of incubation) in which 6MP was predominantly catabolized to 6-thiouric acid and free sulfate, into an intermediate phase (to 8 hr) in which substantial amounts of free 6MP and of ribonucleotides of 6-thioxanthosine and 6-thioguanosine were present while the concentrations of nonnucleotide oxidation products sharply decreased, and into a late phase (to 24 hr) in which the ribonucleotides of 6MP, of 6-thioguanosine and, in particular, of 6-methylthioinosine were the most abundant metabolites."}
{"id": "PMID:476699", "title": "Effect of dietary fiber on the induction of colorectal tumors and fecal beta-glucuronidase activity in the rat.", "content": "The purpose of the present study was to investigate whether three different types of dietary fiber, wheat bran, carrot fiber, and citrus pectin, influenced the induction of colorectal tumors produced by 1,2-dimethylhydrazine in rats. In all groups, the tumor yield was high (87 to 97%). In the wheat bran and carrot fiber groups, the incidence of colorectal tumors was not significantly different from that of the group fed on the fiber-free basic diet. The citrus pectin group, however, had a significantly higher incidence of colorectal tumors (p less than 0.001). An increased number of auditory duct tumors was also noted in this group. In a separate experiment, dietary pectin induced a 10-fold increase in fecal beta-glucuronidase activity but did not alter this activity in the bowel wall. It has been suggested that dietary fiber protects against the induction of colorectal tumors, but this was not the case in the experiment. It is possible that the high tumor yield made the demonstration of a weak protective effect of wheat bran impossible. The reason for the increased occurrence of tumors in the citrus pectin group is obscure and will be subjected to further investigation. Fecal beta-glucuronidase activity might be one factor of importance in the activation of the carcinogen.", "contents": "Effect of dietary fiber on the induction of colorectal tumors and fecal beta-glucuronidase activity in the rat. The purpose of the present study was to investigate whether three different types of dietary fiber, wheat bran, carrot fiber, and citrus pectin, influenced the induction of colorectal tumors produced by 1,2-dimethylhydrazine in rats. In all groups, the tumor yield was high (87 to 97%). In the wheat bran and carrot fiber groups, the incidence of colorectal tumors was not significantly different from that of the group fed on the fiber-free basic diet. The citrus pectin group, however, had a significantly higher incidence of colorectal tumors (p less than 0.001). An increased number of auditory duct tumors was also noted in this group. In a separate experiment, dietary pectin induced a 10-fold increase in fecal beta-glucuronidase activity but did not alter this activity in the bowel wall. It has been suggested that dietary fiber protects against the induction of colorectal tumors, but this was not the case in the experiment. It is possible that the high tumor yield made the demonstration of a weak protective effect of wheat bran impossible. The reason for the increased occurrence of tumors in the citrus pectin group is obscure and will be subjected to further investigation. Fecal beta-glucuronidase activity might be one factor of importance in the activation of the carcinogen."}
{"id": "PMID:476700", "title": "Luminal plasma membrane organization in rat urinary bladder urothelium after direct exposure in vivo to N-methyl-N-nitrosourea.", "content": "Adult female rat urinary bladders were exposed to N-methyl-N-nitrosourea in vivo. After 12 weeks, urothelial hyperplasia was established. Ultrastructural changes of the luminal plasma membrane were studied with thin sectioning and freeze-fracturing techniques. The mosaic pattern which is a characteristic specialization of the normal luminal plasma membrane and of cytoplasmic vesicles disappeared. The plasma membrane of the transformed urothelium was indistinguishable from an undifferentiated membrane in the following respects: thickness; apparent symmetry in cross-section; and distribution of intramembranous particles. Zonulae occludentes became permeable to colloidal lanthanum and appeared disassembled, while desmosomes developed concomitantly. These membrane changes implicate increased permeability and less adaptability of urothelial function to mechanical stress caused by bladder volume changes. The increased desmosome formation may indicate that squamous metaplasia is a feature of early malignant urothelium transformation. It is proposed that N-methyl-N-nitrosourea treatment induces a change in the plasma membrane assembly and specialization.", "contents": "Luminal plasma membrane organization in rat urinary bladder urothelium after direct exposure in vivo to N-methyl-N-nitrosourea. Adult female rat urinary bladders were exposed to N-methyl-N-nitrosourea in vivo. After 12 weeks, urothelial hyperplasia was established. Ultrastructural changes of the luminal plasma membrane were studied with thin sectioning and freeze-fracturing techniques. The mosaic pattern which is a characteristic specialization of the normal luminal plasma membrane and of cytoplasmic vesicles disappeared. The plasma membrane of the transformed urothelium was indistinguishable from an undifferentiated membrane in the following respects: thickness; apparent symmetry in cross-section; and distribution of intramembranous particles. Zonulae occludentes became permeable to colloidal lanthanum and appeared disassembled, while desmosomes developed concomitantly. These membrane changes implicate increased permeability and less adaptability of urothelial function to mechanical stress caused by bladder volume changes. The increased desmosome formation may indicate that squamous metaplasia is a feature of early malignant urothelium transformation. It is proposed that N-methyl-N-nitrosourea treatment induces a change in the plasma membrane assembly and specialization."}
{"id": "PMID:476701", "title": "Enhancement of mammalian cell killing by 5-fluorouracil in combination with X-rays.", "content": "The enhanced cytocidal effect of a combination of X-rays and 5-fluorouracil was investigated by means of colony-forming ability in mouse L-cells. Cells were treated with 5-fluorouracil immediately preceding (preirradiation treatment) or following (postirradiation treatment) irradiation. In either pre- or postirradiation treatment with various concentrations of the drug for a fixed time, the enhanced effect was augmented with increasing concentrations of 5-fluorouracil up to 20 microgram/ml. When cells were subjected to postirradiation treatment with a fixed concentration of drug for varying times, the cytocidal effect was further enhanced with increasing duration of drug treatment. In preirradiation treatment, however, drug treatment for longer than 3 hr did not exhibit any further enhancement. Postirradiation treatment with 5-fluorouracil to synchronous cells at various ages demonstrated enhancement at all ages during the cell cycle. The greatest enhancement was observed in the DNA-synthetic phase. Postirradiation treatment with 5-fluorouracil (2.5 microgram/ml) for 24 hr markedly reduced the width of the shoulder of the X-ray survival curve without significantly altering the slope of the exponential portion of the curve. Recovery from sublethal radiation damage was not suppressed by 5-fluorouracil when cells were treated with drug between fractionated X-ray doses. These results indicate that damages caused by 5-fluorouracil and X-rays interact additively to induce cell killing.", "contents": "Enhancement of mammalian cell killing by 5-fluorouracil in combination with X-rays. The enhanced cytocidal effect of a combination of X-rays and 5-fluorouracil was investigated by means of colony-forming ability in mouse L-cells. Cells were treated with 5-fluorouracil immediately preceding (preirradiation treatment) or following (postirradiation treatment) irradiation. In either pre- or postirradiation treatment with various concentrations of the drug for a fixed time, the enhanced effect was augmented with increasing concentrations of 5-fluorouracil up to 20 microgram/ml. When cells were subjected to postirradiation treatment with a fixed concentration of drug for varying times, the cytocidal effect was further enhanced with increasing duration of drug treatment. In preirradiation treatment, however, drug treatment for longer than 3 hr did not exhibit any further enhancement. Postirradiation treatment with 5-fluorouracil to synchronous cells at various ages demonstrated enhancement at all ages during the cell cycle. The greatest enhancement was observed in the DNA-synthetic phase. Postirradiation treatment with 5-fluorouracil (2.5 microgram/ml) for 24 hr markedly reduced the width of the shoulder of the X-ray survival curve without significantly altering the slope of the exponential portion of the curve. Recovery from sublethal radiation damage was not suppressed by 5-fluorouracil when cells were treated with drug between fractionated X-ray doses. These results indicate that damages caused by 5-fluorouracil and X-rays interact additively to induce cell killing."}
{"id": "PMID:476702", "title": "Effect of splenectomy on the immune response of BALB/c mice bearing an immunoglobulin M plasmacytoma (TEPC-183).", "content": "Mice bearing TEPC-183, an immunoglobulin M(kappa)-secreting plasmacytoma, exhibit severe suppression of their immune responses to both thymus-dependent and thymus-independent antigens, 2,4-dinitrophenyl, and the type 3 pneumococcal polysaccharide SSS-III. This immunosuppression is not lifted by splenectomy of the tumor-bearing mice or prevented by removal of the spleen prior to tumor injection. On the contrary, splenectomy either before or after tumor implantation further accentuates the immunosuppressed state of tumor bearers and even depresses the immune response of normal mice. A secondary immune response of normal mice 34 to 51 days after splenectomy is still reduced. Thus, spleen cells may play a dual role. While splenectomy may remove a source of suppressor cells in tumor-bearing mice, it also eliminates a major source of antibody-producing cells and results in reduced immune responses of normal and TEPC-183-bearing mice. These findings have clinical relevance since splenectomy is used as a therapeutic and diagnostic procedure in neoplastic lymphoproliferative disorders.", "contents": "Effect of splenectomy on the immune response of BALB/c mice bearing an immunoglobulin M plasmacytoma (TEPC-183). Mice bearing TEPC-183, an immunoglobulin M(kappa)-secreting plasmacytoma, exhibit severe suppression of their immune responses to both thymus-dependent and thymus-independent antigens, 2,4-dinitrophenyl, and the type 3 pneumococcal polysaccharide SSS-III. This immunosuppression is not lifted by splenectomy of the tumor-bearing mice or prevented by removal of the spleen prior to tumor injection. On the contrary, splenectomy either before or after tumor implantation further accentuates the immunosuppressed state of tumor bearers and even depresses the immune response of normal mice. A secondary immune response of normal mice 34 to 51 days after splenectomy is still reduced. Thus, spleen cells may play a dual role. While splenectomy may remove a source of suppressor cells in tumor-bearing mice, it also eliminates a major source of antibody-producing cells and results in reduced immune responses of normal and TEPC-183-bearing mice. These findings have clinical relevance since splenectomy is used as a therapeutic and diagnostic procedure in neoplastic lymphoproliferative disorders."}
{"id": "PMID:476703", "title": "Bypassing the \"species barrier\" with carbohydrate-altered interferon from leukocytes.", "content": "Interferon, a glycoprotein with demonstrated antitumor and antiviral properties, can be obtained from cells of different species, and some of these interferons can be effective across species lines. I hypothesize that the ability to cross or not to cross species lines lies in the carbohydrate moiety, and cross-species biological activity is a property of the polypeptide. This would represent one of the first major roles uncovered for glycosylation in any biological system. If this hypothesis is correct, then it should be possible to use animal-derived interferon as a large scale, inexpensive source to treat human diseases.", "contents": "Bypassing the \"species barrier\" with carbohydrate-altered interferon from leukocytes. Interferon, a glycoprotein with demonstrated antitumor and antiviral properties, can be obtained from cells of different species, and some of these interferons can be effective across species lines. I hypothesize that the ability to cross or not to cross species lines lies in the carbohydrate moiety, and cross-species biological activity is a property of the polypeptide. This would represent one of the first major roles uncovered for glycosylation in any biological system. If this hypothesis is correct, then it should be possible to use animal-derived interferon as a large scale, inexpensive source to treat human diseases."}
{"id": "PMID:476704", "title": "Multiple myeloma resistant to melphalan: treatment with cyclophosphamide, prednisone, and BCNU.", "content": "Eighty-nine patients with multiple myeloma resistant to melphalan were randomized to receive cyclophosphamide plus prednisone (CP) (47 patients) or cyclophosphamide plus BCNU plus prednisone (CBP) (42 patients). No differences were detected in the two groups prior to therapy. Objective responses occurred in three (7%) of the CP patients and in seven (17%) of the CBP patients. About 40% of the patients in each group achieved some response. Toxic reactions consisted mainly of leukopenia and thrombocytopenia. Median survival was not different in the two groups. The median survival time was 31 months among those patients with an objective response and 9.4 months among those without an objective response. The addition of BCNU to CP increased the frequency of objective response, but not significantly. This triple combination (CBP) cannot be recommended.", "contents": "Multiple myeloma resistant to melphalan: treatment with cyclophosphamide, prednisone, and BCNU. Eighty-nine patients with multiple myeloma resistant to melphalan were randomized to receive cyclophosphamide plus prednisone (CP) (47 patients) or cyclophosphamide plus BCNU plus prednisone (CBP) (42 patients). No differences were detected in the two groups prior to therapy. Objective responses occurred in three (7%) of the CP patients and in seven (17%) of the CBP patients. About 40% of the patients in each group achieved some response. Toxic reactions consisted mainly of leukopenia and thrombocytopenia. Median survival was not different in the two groups. The median survival time was 31 months among those patients with an objective response and 9.4 months among those without an objective response. The addition of BCNU to CP increased the frequency of objective response, but not significantly. This triple combination (CBP) cannot be recommended."}
{"id": "PMID:476705", "title": "Association between steroid hormone receptor status and disease-free interval in breast cancer.", "content": "The possibility of an association between steroid hormone receptor status and disease-free interval was examined in 292 patients with breast cancer. Estrogen receptor positivity was associated with a prolonged disease-free interval. This association was independent of age, menopausal status, tumor size, or nodal status. There was no association between the presence or absence of progesterone, androgen, or glucocorticoid receptor and disease-free interval.", "contents": "Association between steroid hormone receptor status and disease-free interval in breast cancer. The possibility of an association between steroid hormone receptor status and disease-free interval was examined in 292 patients with breast cancer. Estrogen receptor positivity was associated with a prolonged disease-free interval. This association was independent of age, menopausal status, tumor size, or nodal status. There was no association between the presence or absence of progesterone, androgen, or glucocorticoid receptor and disease-free interval."}
{"id": "PMID:476706", "title": "Mechanisms of sensitivity or resistance of murine tumors to N-(phosphonacetyl)-L-aspartate (PALA).", "content": "Several biochemical parameters were examined relative to the sensitivity or resistance of representative rodent tumors to N-(phosphonacetyl)-L-aspartate (PALA). The activity of the target enzyme, L-aspartate transcarbamylase (ATCase), was evaluated in homogenates of a spectrum of murine neoplasms. ATCase activity was significantly lower in PALA-sensitive as opposed to PALA-refractory tumors. However, among tumors sensitive to PALA, there was no clearcut relationship between ATCase activity and degree of sensitivity to PALA. Thus, a number of hypotheses were proposed to explain differential sensitivity to PALA in vivo. Enzyme activities in the salvage pathway which phosphorylate pyrimidine nucleosides and deoxynucleosides were found to be greater in refractory tumors. The uptake of PALA, in vitro, though quite slow, was found to be two to eight times greater in two sensitive tumors as compared to the refractory L1210 leukemia. However, in vivo, 24 hours following graduated doses of PALA, nearly identical intratumoral drug concentrations were observed in representative sensitive and refractory tumors. Thus, ultimately, PALA transport would not appear to correlate with differences in drug sensitivity. A number of other biochemical parameters were also found to have no association with sensitivity to PALA in vivo. These included: kinetics of inhibition of ATCase, capacity for restitution of ATCase activity after a dose of PALA, degree of inhibition of ATCase at various doses of PALA, detoxification of PALA by tumor cells, kinetics of uptake of uridine, or catabolism of pyrimidines or pyrimidine nucleosides.", "contents": "Mechanisms of sensitivity or resistance of murine tumors to N-(phosphonacetyl)-L-aspartate (PALA). Several biochemical parameters were examined relative to the sensitivity or resistance of representative rodent tumors to N-(phosphonacetyl)-L-aspartate (PALA). The activity of the target enzyme, L-aspartate transcarbamylase (ATCase), was evaluated in homogenates of a spectrum of murine neoplasms. ATCase activity was significantly lower in PALA-sensitive as opposed to PALA-refractory tumors. However, among tumors sensitive to PALA, there was no clearcut relationship between ATCase activity and degree of sensitivity to PALA. Thus, a number of hypotheses were proposed to explain differential sensitivity to PALA in vivo. Enzyme activities in the salvage pathway which phosphorylate pyrimidine nucleosides and deoxynucleosides were found to be greater in refractory tumors. The uptake of PALA, in vitro, though quite slow, was found to be two to eight times greater in two sensitive tumors as compared to the refractory L1210 leukemia. However, in vivo, 24 hours following graduated doses of PALA, nearly identical intratumoral drug concentrations were observed in representative sensitive and refractory tumors. Thus, ultimately, PALA transport would not appear to correlate with differences in drug sensitivity. A number of other biochemical parameters were also found to have no association with sensitivity to PALA in vivo. These included: kinetics of inhibition of ATCase, capacity for restitution of ATCase activity after a dose of PALA, degree of inhibition of ATCase at various doses of PALA, detoxification of PALA by tumor cells, kinetics of uptake of uridine, or catabolism of pyrimidines or pyrimidine nucleosides."}
{"id": "PMID:476707", "title": "Role of plasma carcinoembryonic antigen in evaluating patients with breast cancer treated with adjuvant chemotherapy.", "content": "Serial plasma carcinoembryonic antigen (CEA) levels were determined in 84 patients with breast cancer who were receiving postsurgical adjuvant chemoimmunotherapy or adjuvant chemotherapy. CEA values were correlated with clinical status, scintiscans, alkaline phosphatase, LDH, and SGOT. CEA values greater than 2.5 ng/ml were considered abnormal. Thirty patients had normal serial CEA values; all remain disease-free. In 54 patients one or more abnormal CEA values were recorded; nine of 54 developed overt metastatic disease. Relapses occurred in four of 38 patients in whom values up to 5 ng/ml were recorded, in three of 14 patients in whom values up to 10 ng/ml were recorded, and in two of two patients in whom values greater than 10 ng/ml were recorded.", "contents": "Role of plasma carcinoembryonic antigen in evaluating patients with breast cancer treated with adjuvant chemotherapy. Serial plasma carcinoembryonic antigen (CEA) levels were determined in 84 patients with breast cancer who were receiving postsurgical adjuvant chemoimmunotherapy or adjuvant chemotherapy. CEA values were correlated with clinical status, scintiscans, alkaline phosphatase, LDH, and SGOT. CEA values greater than 2.5 ng/ml were considered abnormal. Thirty patients had normal serial CEA values; all remain disease-free. In 54 patients one or more abnormal CEA values were recorded; nine of 54 developed overt metastatic disease. Relapses occurred in four of 38 patients in whom values up to 5 ng/ml were recorded, in three of 14 patients in whom values up to 10 ng/ml were recorded, and in two of two patients in whom values greater than 10 ng/ml were recorded."}
{"id": "PMID:476710", "title": "Phase II trial of piperazinedione in malignant melanoma: a report by the Southeastern Cancer Study Group.", "content": "Forty-one evaluable patients with malignant melanoma resistant to other chemotherapeutic agents received 9 mg/m2 of piperazinedione every 3 weeks. Two patients had partial responses and one additional patient had stable disease. One of the partial responses occurred in a patient with subcutaneous metastases and the other occurred in a patient with pulmonary and osseous metastases. Both antitumor responses occurred in the 17 patients with a performance status of greater than or equal to 80%. The dose-limiting toxicity was myelosuppression; thrombocytopenia was more frequently observed than granulocytopenia.", "contents": "Phase II trial of piperazinedione in malignant melanoma: a report by the Southeastern Cancer Study Group. Forty-one evaluable patients with malignant melanoma resistant to other chemotherapeutic agents received 9 mg/m2 of piperazinedione every 3 weeks. Two patients had partial responses and one additional patient had stable disease. One of the partial responses occurred in a patient with subcutaneous metastases and the other occurred in a patient with pulmonary and osseous metastases. Both antitumor responses occurred in the 17 patients with a performance status of greater than or equal to 80%. The dose-limiting toxicity was myelosuppression; thrombocytopenia was more frequently observed than granulocytopenia."}
{"id": "PMID:476711", "title": "Phase II study demonstrating failure of both a five-drug continuous-therapy regimen and a two-drug pulse-therapy regimen in the treatment of metastatic neuroblastoma: Southwest Oncology Group Study 822.", "content": "Twenty-six children greater than 1 year of age with previously untreated stage IV neuroblastoma were randomized to receive either a five-drug regimen (prednisone, cyclophosphamide, actinomycin D, vincristine, and daunorubicin) or a two-drug regimen (cyclophosphamide and vincristine). Complete response rates were 6% and 9% for the five-drug and the two-drug regimens respectively. Partial response rates were 13% and 27% for the five-drug and the two-drug regimens respectively. The mean duration of the seven responses was 9 months, and all 26 patients had died within 2 years. Neither regimen was effective for long-term disease control in these children with neuroblastoma.", "contents": "Phase II study demonstrating failure of both a five-drug continuous-therapy regimen and a two-drug pulse-therapy regimen in the treatment of metastatic neuroblastoma: Southwest Oncology Group Study 822. Twenty-six children greater than 1 year of age with previously untreated stage IV neuroblastoma were randomized to receive either a five-drug regimen (prednisone, cyclophosphamide, actinomycin D, vincristine, and daunorubicin) or a two-drug regimen (cyclophosphamide and vincristine). Complete response rates were 6% and 9% for the five-drug and the two-drug regimens respectively. Partial response rates were 13% and 27% for the five-drug and the two-drug regimens respectively. The mean duration of the seven responses was 9 months, and all 26 patients had died within 2 years. Neither regimen was effective for long-term disease control in these children with neuroblastoma."}
{"id": "PMID:476709", "title": "Phase II evaluation of diglycoaldehyde, VP-16-213, and the combination of methyl-CCNU and beta-2'-deoxythioguanosine in previously treated patients with colorectal cancer: an Eastern Cooperative Oncology Group study (EST-1275).", "content": "The Eastern Cooperative Oncology Group assessed the activity of diglycoaldehyde (DGA), VP-16-213, and the combination of methyl-CCNU and beta-2'-deoxythioguanosine in previously treated patients with advanced colorectal cancer. Objective responses were seen in two of 40 evaluable patients receiving methyl-CCNU and beta-2'-deoxythioguanosine and in one of 35 patients receiving DGA. None of 33 patients responded to VP-16-213, but one death related to sepsis and bone marrow failure occurred. Survival of patients whose previous chemotherapy included a nitrosourea was markedly shortened compared to those who had not been exposed to nitrosoureas. With the possible exception of DGA, further treatment of patients with colorectal cancer with these therapies is not warranted.", "contents": "Phase II evaluation of diglycoaldehyde, VP-16-213, and the combination of methyl-CCNU and beta-2'-deoxythioguanosine in previously treated patients with colorectal cancer: an Eastern Cooperative Oncology Group study (EST-1275). The Eastern Cooperative Oncology Group assessed the activity of diglycoaldehyde (DGA), VP-16-213, and the combination of methyl-CCNU and beta-2'-deoxythioguanosine in previously treated patients with advanced colorectal cancer. Objective responses were seen in two of 40 evaluable patients receiving methyl-CCNU and beta-2'-deoxythioguanosine and in one of 35 patients receiving DGA. None of 33 patients responded to VP-16-213, but one death related to sepsis and bone marrow failure occurred. Survival of patients whose previous chemotherapy included a nitrosourea was markedly shortened compared to those who had not been exposed to nitrosoureas. With the possible exception of DGA, further treatment of patients with colorectal cancer with these therapies is not warranted."}
{"id": "PMID:476712", "title": "ICRF-159 (razoxane) in the treatment of pediatric solid tumors: a Southwest Oncology Group study.", "content": "ICRF-159 is active in several animal tumor model systems and human adult malignancies. In this phase II study, ICRF-159 was given on a weekly schedule, 3000 mg/m2/day, orally in three divided doses at 6-hour intervals to 78 children with a variety of malignant neoplasms. Fifty-three patients were evaluable for tumor response. Toxicity was primarily hematopoietic and gastrointestinal. There were no responses in any of the eight patients with osteogenic sarcoma, four with lymphoma, five with Ewing's sarcoma, ten with neuroblastoma, or six with rhabdomyosarcoma. There was a transient partial response in one of four children with Wilms' tumor. Further trials with this drug using this schedule are not indicated for the common childhood solid tumors.", "contents": "ICRF-159 (razoxane) in the treatment of pediatric solid tumors: a Southwest Oncology Group study. ICRF-159 is active in several animal tumor model systems and human adult malignancies. In this phase II study, ICRF-159 was given on a weekly schedule, 3000 mg/m2/day, orally in three divided doses at 6-hour intervals to 78 children with a variety of malignant neoplasms. Fifty-three patients were evaluable for tumor response. Toxicity was primarily hematopoietic and gastrointestinal. There were no responses in any of the eight patients with osteogenic sarcoma, four with lymphoma, five with Ewing's sarcoma, ten with neuroblastoma, or six with rhabdomyosarcoma. There was a transient partial response in one of four children with Wilms' tumor. Further trials with this drug using this schedule are not indicated for the common childhood solid tumors."}
{"id": "PMID:476715", "title": "Combination chemotherapy for extracranial metastases of a primary malignant cerebral neoplasm.", "content": "A patient with a mixed malignant glioma metastatic to the bones and lungs failed to respond to two different broad-spectrum combination chemotherapy regimens which included Adriamycin, methotrexate, cyclophosphamide, CCNU and cis-dichlorodiammineplatinum(II).", "contents": "Combination chemotherapy for extracranial metastases of a primary malignant cerebral neoplasm. A patient with a mixed malignant glioma metastatic to the bones and lungs failed to respond to two different broad-spectrum combination chemotherapy regimens which included Adriamycin, methotrexate, cyclophosphamide, CCNU and cis-dichlorodiammineplatinum(II)."}
{"id": "PMID:476718", "title": "Structural investigations of the lipopolysaccharide isolated from Vibrio cholera, Inaba 569 B.", "content": "On hydrolysis, the purified lipopolysaccharide (LPS) isolated from Vibrio cholera, Inaba 569 B, yielded glucose, mannose, a heptose behaving like D-glycero-L-manno-heptose and one behaving like D-glycero-L-gluco-heptose, 2-amino-2-deoxyglucose, and glucuronic acid in the molar ratios of approximately 9:4:5:1:2:5. Studies on the LPS, the polysaccharide (PS), and carboxyl-reduced LPS showed that the PS has a branched structure, with (1 leads to 2)-linked mannopyranosyl and a heptopyranosyl, and (1 leads to 4)-linked glucopyranosyluronic and 2-amino-2-deoxyglucopyranosyl residues in the interior part of the molecule, and glucopyranosyl and heptopyranosyl residues as nonreducing end-groups.", "contents": "Structural investigations of the lipopolysaccharide isolated from Vibrio cholera, Inaba 569 B. On hydrolysis, the purified lipopolysaccharide (LPS) isolated from Vibrio cholera, Inaba 569 B, yielded glucose, mannose, a heptose behaving like D-glycero-L-manno-heptose and one behaving like D-glycero-L-gluco-heptose, 2-amino-2-deoxyglucose, and glucuronic acid in the molar ratios of approximately 9:4:5:1:2:5. Studies on the LPS, the polysaccharide (PS), and carboxyl-reduced LPS showed that the PS has a branched structure, with (1 leads to 2)-linked mannopyranosyl and a heptopyranosyl, and (1 leads to 4)-linked glucopyranosyluronic and 2-amino-2-deoxyglucopyranosyl residues in the interior part of the molecule, and glucopyranosyl and heptopyranosyl residues as nonreducing end-groups."}
{"id": "PMID:476722", "title": "An alternative synthesis of 4-deoxy-4-fluoro-D-glucose and its transport in the human erythrocyte.", "content": "Treatment of methyl 4-O-mesy-alpha-D-galactopyranoside with benzyl bromide in N,N-dimethylformamide in the presence of silver oxide yielded methyl 2,3,6-tri-O-benzyl-4-O-mesyl-alpha-D-galactopyranoside which with tert-butylammonium fluoride at reflux underwent nucleophilic displacement to give methyl 2,3,6-tri-O-benzyl-4-deoxy-4-fluoro-alpha-D-glucopyranoside. This compound on hydrogenolysis provided crystalline methyl 4-deoxy-4-fluoro-alpha-D-glucopyranoside (9). The structure of 9 was established by its conversion to the 2,3,6-tri-O-acetyl derivative and by n.m.r. and m.s. analysis. Acid hydrolysis of 9 gave 4-deoxy-4-fluoro-D-glucose (1). A modification of an established synthesis of 4-deoxy-D-xylo-hexose (2) from methyl 2,3,6-tri-O-benzoyl-alpha-D-galactopyranoside is described. A systematic comparison was made of the transport parameters (Kx and Vmax) of D-glucose, 2, and 1 in human erythrocytes. The Kx values observed for the above sugars are: 4.0mM, 4.5mM, and 4.6mM, respectively. These results indicate that O-4 in beta-D-glucopyranose is not involved in hydrogen bonding to the carrier protein associated with the transport of D-glucose in the erythrocyte.", "contents": "An alternative synthesis of 4-deoxy-4-fluoro-D-glucose and its transport in the human erythrocyte. Treatment of methyl 4-O-mesy-alpha-D-galactopyranoside with benzyl bromide in N,N-dimethylformamide in the presence of silver oxide yielded methyl 2,3,6-tri-O-benzyl-4-O-mesyl-alpha-D-galactopyranoside which with tert-butylammonium fluoride at reflux underwent nucleophilic displacement to give methyl 2,3,6-tri-O-benzyl-4-deoxy-4-fluoro-alpha-D-glucopyranoside. This compound on hydrogenolysis provided crystalline methyl 4-deoxy-4-fluoro-alpha-D-glucopyranoside (9). The structure of 9 was established by its conversion to the 2,3,6-tri-O-acetyl derivative and by n.m.r. and m.s. analysis. Acid hydrolysis of 9 gave 4-deoxy-4-fluoro-D-glucose (1). A modification of an established synthesis of 4-deoxy-D-xylo-hexose (2) from methyl 2,3,6-tri-O-benzoyl-alpha-D-galactopyranoside is described. A systematic comparison was made of the transport parameters (Kx and Vmax) of D-glucose, 2, and 1 in human erythrocytes. The Kx values observed for the above sugars are: 4.0mM, 4.5mM, and 4.6mM, respectively. These results indicate that O-4 in beta-D-glucopyranose is not involved in hydrogen bonding to the carrier protein associated with the transport of D-glucose in the erythrocyte."}
{"id": "PMID:476723", "title": "The structure of the capsular polysaccharide of Klebsiella K-type 31.", "content": "The structure of the Klebsiella K31 capsular polysaccharide has been elucidated by periodate oxidation, methylation analysis, characterization of oligosaccharides obtained by partial, acid hydrolysis, and proton magnetic resonance. The polymer consists of pentasaccharide repeating-units having the following structure (1).", "contents": "The structure of the capsular polysaccharide of Klebsiella K-type 31. The structure of the Klebsiella K31 capsular polysaccharide has been elucidated by periodate oxidation, methylation analysis, characterization of oligosaccharides obtained by partial, acid hydrolysis, and proton magnetic resonance. The polymer consists of pentasaccharide repeating-units having the following structure (1)."}
{"id": "PMID:476724", "title": "Protection of sodium dodecyl sulfate-induced aggregation of concanalvalin A by saccharide ligands.", "content": "Concanavalin A is visibly aggregated by low concentrations of sodium dodecyl sulfate, maximum aggregation being obtained at pH 4.6. Other denaturants, such as urea, guanidine hydrochloride, Triton X-100, cetyltrimethylammonium bromide, Tween 80, and Brij 35 are ineffective in promoting visible aggregation. The sodium dodecyl sulfate-induced aggregation of concanavalin A requires the presence of an intact, saccharide-ligand binding-site. Rapid and complete reversal of the detergent effect was achieved by use of saccharides which bind to the lectin. Such compounds as tryptophan and o-nitrophenyl beta-D-galactopyranoside did not inhibit the aggregation of concanavalin A by sodium dodecyl sulfate, suggesting that the detergent does not bind the hydrophobic pocket on the surface of the protein. The results suggest that concanavalin A may have an additional, ligand-binding site which is metal-dependent and which can be modified by the addition of a saccharide ligand.", "contents": "Protection of sodium dodecyl sulfate-induced aggregation of concanalvalin A by saccharide ligands. Concanavalin A is visibly aggregated by low concentrations of sodium dodecyl sulfate, maximum aggregation being obtained at pH 4.6. Other denaturants, such as urea, guanidine hydrochloride, Triton X-100, cetyltrimethylammonium bromide, Tween 80, and Brij 35 are ineffective in promoting visible aggregation. The sodium dodecyl sulfate-induced aggregation of concanavalin A requires the presence of an intact, saccharide-ligand binding-site. Rapid and complete reversal of the detergent effect was achieved by use of saccharides which bind to the lectin. Such compounds as tryptophan and o-nitrophenyl beta-D-galactopyranoside did not inhibit the aggregation of concanavalin A by sodium dodecyl sulfate, suggesting that the detergent does not bind the hydrophobic pocket on the surface of the protein. The results suggest that concanavalin A may have an additional, ligand-binding site which is metal-dependent and which can be modified by the addition of a saccharide ligand."}
{"id": "PMID:476725", "title": "The binding of aromatic derivatives of beta-D-galactopyranosides to the FAB' of immunoglobulin J539. Observations on the nature of ligand-antibody interactions.", "content": "A number of D-galactopyranosides bearing aromatic substituents have been prepared, and their binding to immunoglobulin J539 (Fab') has been studied. It appears that the main contribution of the 6-O-aromatic moiety to binding arises from the fact that it imparts an increased hydrophobicity to the ligand, causing a decrease in its hydration (solubility) that results in a greater free-energy of binding. In the D-galactosides having an aromatic aglycon, the phenyl group appears to partake in actual interactions with the protein.", "contents": "The binding of aromatic derivatives of beta-D-galactopyranosides to the FAB' of immunoglobulin J539. Observations on the nature of ligand-antibody interactions. A number of D-galactopyranosides bearing aromatic substituents have been prepared, and their binding to immunoglobulin J539 (Fab') has been studied. It appears that the main contribution of the 6-O-aromatic moiety to binding arises from the fact that it imparts an increased hydrophobicity to the ligand, causing a decrease in its hydration (solubility) that results in a greater free-energy of binding. In the D-galactosides having an aromatic aglycon, the phenyl group appears to partake in actual interactions with the protein."}
{"id": "PMID:476726", "title": "Improved purification procedure for the extracellular D-glucosyltransferase from Streptococcus mutans 6715.", "content": "The exocellular D-glucosyltransferase from Streptococcus mutans 6715 has been highly purified with minimal loss of enzymic activity. The organisms were cultured in trypticase soy-broth that had been treated with invertase and filtered through an ultrafilter fitted with a membrane having a cut-off molecular weight at 10,000. To the growth medium was added Tween 80, which prevented the enzyme from aggregating. The final step in the purification employed insoluble, streptococcal dextran as an affinity support. Two D-glucosyltransferase activities were detected, viz., one that did not adsorb to the insoluble dextran and one that did. The enzymic fraction that had adsorbed to the insoluble dextran in the affinity column was strongly inhibited by added insoluble dextran.", "contents": "Improved purification procedure for the extracellular D-glucosyltransferase from Streptococcus mutans 6715. The exocellular D-glucosyltransferase from Streptococcus mutans 6715 has been highly purified with minimal loss of enzymic activity. The organisms were cultured in trypticase soy-broth that had been treated with invertase and filtered through an ultrafilter fitted with a membrane having a cut-off molecular weight at 10,000. To the growth medium was added Tween 80, which prevented the enzyme from aggregating. The final step in the purification employed insoluble, streptococcal dextran as an affinity support. Two D-glucosyltransferase activities were detected, viz., one that did not adsorb to the insoluble dextran and one that did. The enzymic fraction that had adsorbed to the insoluble dextran in the affinity column was strongly inhibited by added insoluble dextran."}
{"id": "PMID:476727", "title": "Vascular hemodynamics: deep rooted misconceptions misnomers.", "content": "Erroneous concepts about vascular hemodynamics are widespread, notably as regards the effect of gravity on blood flow. Vascular pressure at any point is equal to the sum of two pressures of entirely different origins: (1) pressure caused by the pumping action of the heart ('cardiodynamic' pressure which is vital for circulation) and (2) pressure due to gravity acting on the blood ('gravitational' or hydrostatic pressure which plays no direct role in blood flow). Gravity neither helps nor hinders circulation because of the U tube or siphon principle. The gravitational energy of the column of blood in arteries is balanced exactly by the gravitational energy of the column in veins and vice versa. Thus, contrary to common belief, gravity does not hinder blood flow to the head in the upright position, nor does it hinder venous return from the dependent parts of the body. For this reason, in Poiseuille's equation, perfusion pressure should exclude gravitational pressure. Postural effects on circulation result from the distension vessels (particulary veins) subsequent to changes in gravitational pressure of blood.", "contents": "Vascular hemodynamics: deep rooted misconceptions misnomers. Erroneous concepts about vascular hemodynamics are widespread, notably as regards the effect of gravity on blood flow. Vascular pressure at any point is equal to the sum of two pressures of entirely different origins: (1) pressure caused by the pumping action of the heart ('cardiodynamic' pressure which is vital for circulation) and (2) pressure due to gravity acting on the blood ('gravitational' or hydrostatic pressure which plays no direct role in blood flow). Gravity neither helps nor hinders circulation because of the U tube or siphon principle. The gravitational energy of the column of blood in arteries is balanced exactly by the gravitational energy of the column in veins and vice versa. Thus, contrary to common belief, gravity does not hinder blood flow to the head in the upright position, nor does it hinder venous return from the dependent parts of the body. For this reason, in Poiseuille's equation, perfusion pressure should exclude gravitational pressure. Postural effects on circulation result from the distension vessels (particulary veins) subsequent to changes in gravitational pressure of blood."}
{"id": "PMID:476728", "title": "Complete atrio-ventricular block due to cardiac echinococcosis.", "content": "A 33-year-old man presented a complete antrio-ventricular block due to two hydatid cysts localized in the interventricular septum and interrupting both bundle branches. Intracardiac rupture within the right ventricle led to extensive pulmonary hydatidosis and death.", "contents": "Complete atrio-ventricular block due to cardiac echinococcosis. A 33-year-old man presented a complete antrio-ventricular block due to two hydatid cysts localized in the interventricular septum and interrupting both bundle branches. Intracardiac rupture within the right ventricle led to extensive pulmonary hydatidosis and death."}
{"id": "PMID:476730", "title": "Differences in atrial septal activation with an intrasinoatrial nodal pacemaker and epicardial sinoatrial nodal pacing.", "content": "Changes in Intra-SA nodal pacemaker localization were produced through stimulation of the decentralized cervical vagi and stellate ganglia in the anesthetized dog. Shifts in pacemaker to the rostral, middle, or caudal regions of the SA node produced a change in the timing as well as a change in the sequence of activation of recording sites overlying the AV node. Epicardial pacing with a plaque electrode from either the rostral, middle, or caudal regions of the SA node produced the same activation sequence of the AV nodal electrodes irrespective of the epicardial SA nodal pacing site. The inability of epicardial SA nodal pacing to precisely reproduce the activation pattern of the atrial septum overlying the AV node observed with a natural SA nodal pacemaker can be explained by the geographic relationship of the pacemaker cells within the node to the preferential internodal pathways and the area of atrial tissue stimulated by pacing. Pacing activates a large mass of tissue, whereas an intrinsic pacemaker probably acts as a more localized focus. The inability of pacing to reproduce the activation pattern seen with spontaneous rhythm may be a determinant in the varied P wave morphology seen with coronary sinus or AV nodal junctional rhythms, as compared with more consistent morphology seen with pacing.", "contents": "Differences in atrial septal activation with an intrasinoatrial nodal pacemaker and epicardial sinoatrial nodal pacing. Changes in Intra-SA nodal pacemaker localization were produced through stimulation of the decentralized cervical vagi and stellate ganglia in the anesthetized dog. Shifts in pacemaker to the rostral, middle, or caudal regions of the SA node produced a change in the timing as well as a change in the sequence of activation of recording sites overlying the AV node. Epicardial pacing with a plaque electrode from either the rostral, middle, or caudal regions of the SA node produced the same activation sequence of the AV nodal electrodes irrespective of the epicardial SA nodal pacing site. The inability of epicardial SA nodal pacing to precisely reproduce the activation pattern of the atrial septum overlying the AV node observed with a natural SA nodal pacemaker can be explained by the geographic relationship of the pacemaker cells within the node to the preferential internodal pathways and the area of atrial tissue stimulated by pacing. Pacing activates a large mass of tissue, whereas an intrinsic pacemaker probably acts as a more localized focus. The inability of pacing to reproduce the activation pattern seen with spontaneous rhythm may be a determinant in the varied P wave morphology seen with coronary sinus or AV nodal junctional rhythms, as compared with more consistent morphology seen with pacing."}
{"id": "PMID:476731", "title": "Familial Ebstein's anomaly.", "content": "2 adult siblings with Ebstein's anomaly are presented in this report. The second sibling was found to have the anomaly 20 years after the first sibling was diagnosed. The use of echocardiography in screening for Ebstein's anomaly and the diagnostic features are emphasized. Documentation by electrode catheter recording intracardiac electrocardiogram and pressures in the right heart were made.", "contents": "Familial Ebstein's anomaly. 2 adult siblings with Ebstein's anomaly are presented in this report. The second sibling was found to have the anomaly 20 years after the first sibling was diagnosed. The use of echocardiography in screening for Ebstein's anomaly and the diagnostic features are emphasized. Documentation by electrode catheter recording intracardiac electrocardiogram and pressures in the right heart were made."}
{"id": "PMID:476732", "title": "Effect of a physical conditioning program upon left ventricular ejection fractions determined serially by a noninvasive technique.", "content": "6 clinically normal subjects underwent a 3-month physical conditioning program with the ejection fractions determined before and after physical conditioning using a scintillation probe. All subjects achieved a conditioning effect as evidenced by increased treadmill test duration after conditioning (mean duration before conditioning: 658 vs. 715 sec after conditioning; p less than 0.02). All 6 subjects increased resting ejection fractions after conditioning (mean ejection fraction before conditioning: 54.5 +/- 5.4%; mean ejection fraction after conditioning: 67.0 +/- 9.0%; p less than 0.01). Thus, an aerobic physical conditioning program appears to increase resting ejection fractions in normal subjects.", "contents": "Effect of a physical conditioning program upon left ventricular ejection fractions determined serially by a noninvasive technique. 6 clinically normal subjects underwent a 3-month physical conditioning program with the ejection fractions determined before and after physical conditioning using a scintillation probe. All subjects achieved a conditioning effect as evidenced by increased treadmill test duration after conditioning (mean duration before conditioning: 658 vs. 715 sec after conditioning; p less than 0.02). All 6 subjects increased resting ejection fractions after conditioning (mean ejection fraction before conditioning: 54.5 +/- 5.4%; mean ejection fraction after conditioning: 67.0 +/- 9.0%; p less than 0.01). Thus, an aerobic physical conditioning program appears to increase resting ejection fractions in normal subjects."}
{"id": "PMID:476733", "title": "Beneficial effects of ibuprofen in acute myocardial ischemia.", "content": "Ibuprofen, a nonsteriodal anti-inflammatory agent, was studied in the early stages of myocardial ischemia in order to determine whether it helps preserve myocardial integrity. Ibuprofen was administered intravenously at a dose of 12.5 mg/kg at the time of coronary artery occlusion and again 2.5 h later. Ibuprofen significantly prevented the loss of myocardial creatine phosphokinase (CPK) release in ischemic cardiac tissue. In addition, this drug significantly returned S-T segment elevation toward normal values, and significantly prevented the myocardial loss of compounds having free amino nitrogen groups, an index of proteolysis. Although ibuprofen moderated the increased plasma CPK activity, plasma CPK values 5 h after coronary occlusion were above control values. Thus, ibuprofen significantly prevented alterations in three of the four indices used to assess myocardial ischemic damage. The protective mechanism of ibuprofen may be via stabilization of cellular membranes (i.e., lysosomal membranes) and to a lesser extent on reduction in myocardial oxygen demand.", "contents": "Beneficial effects of ibuprofen in acute myocardial ischemia. Ibuprofen, a nonsteriodal anti-inflammatory agent, was studied in the early stages of myocardial ischemia in order to determine whether it helps preserve myocardial integrity. Ibuprofen was administered intravenously at a dose of 12.5 mg/kg at the time of coronary artery occlusion and again 2.5 h later. Ibuprofen significantly prevented the loss of myocardial creatine phosphokinase (CPK) release in ischemic cardiac tissue. In addition, this drug significantly returned S-T segment elevation toward normal values, and significantly prevented the myocardial loss of compounds having free amino nitrogen groups, an index of proteolysis. Although ibuprofen moderated the increased plasma CPK activity, plasma CPK values 5 h after coronary occlusion were above control values. Thus, ibuprofen significantly prevented alterations in three of the four indices used to assess myocardial ischemic damage. The protective mechanism of ibuprofen may be via stabilization of cellular membranes (i.e., lysosomal membranes) and to a lesser extent on reduction in myocardial oxygen demand."}
{"id": "PMID:476734", "title": "Incidence and prognostic significance of intraventricular block in acute myocardial infarction.", "content": "In 404 consecutively admitted patients with their first myocardial infarction (MI), intraventricular block (IV) was a complication in 124 (31%). The following types of block were encountered: 21 (5%) had left bundle-branch block (LBBB), 73 (18%) left anterior hemiblock (LAH), 13 (3%) left posterior hemiblock (LPH); 7 (2%) right bundle-branch block (RBBB); 9 (2%) RBBB + LAH, and 1 (0.3%) RBBB + LPH. Patients with IV block at the time of admission did not develop total atrioventricular block more frequently in the acute phase of MI (0-30 days) or in the follow-up period (3-5 years) than patients without IV block. During the acute phase, only patients with RBBB with or without hemiblock showed significantly higher mortality than patients without IV blocks. The other types of IV block did not influence the short-term prognosis. Among patients who survived the acute phase, significantly lower long-term survival rates were found in patients with LBBB compared to patients without IV block, whereas the presence of LAH did not affect the long-term prognosis.", "contents": "Incidence and prognostic significance of intraventricular block in acute myocardial infarction. In 404 consecutively admitted patients with their first myocardial infarction (MI), intraventricular block (IV) was a complication in 124 (31%). The following types of block were encountered: 21 (5%) had left bundle-branch block (LBBB), 73 (18%) left anterior hemiblock (LAH), 13 (3%) left posterior hemiblock (LPH); 7 (2%) right bundle-branch block (RBBB); 9 (2%) RBBB + LAH, and 1 (0.3%) RBBB + LPH. Patients with IV block at the time of admission did not develop total atrioventricular block more frequently in the acute phase of MI (0-30 days) or in the follow-up period (3-5 years) than patients without IV block. During the acute phase, only patients with RBBB with or without hemiblock showed significantly higher mortality than patients without IV blocks. The other types of IV block did not influence the short-term prognosis. Among patients who survived the acute phase, significantly lower long-term survival rates were found in patients with LBBB compared to patients without IV block, whereas the presence of LAH did not affect the long-term prognosis."}
{"id": "PMID:476735", "title": "Sudden coronary death and coronary artery disease. A clinicopathologic appraisal.", "content": "Clinicopathologic and epidemiological aspects of coronary artery disease (CAD) in relation to sudden death (SD) were studied in three postmortem series, two of sudden cardiac death and one of male violent death. There appears to be a critical level of severity of CAD which determines the risk of SD. The more severe the CAD within this range, the higher is the risk. Every annual cohort of SDs includes many patients with extremely severe, or 'burned-out', disease. CAD alone, however, is not a selective factor for sudden or not-sudden death. Epidemiological analysis (a) determines the age and frequency of persons reaching the critical risk level of CAD severity in the population (atherogenic factors); (b) influences the selection of the SD victims from the critical population (precipitating factors), and (c) determines the individual variation of CAD severity with which coronary heart disease and SD manifest themselves (sensitising and protecting factors).", "contents": "Sudden coronary death and coronary artery disease. A clinicopathologic appraisal. Clinicopathologic and epidemiological aspects of coronary artery disease (CAD) in relation to sudden death (SD) were studied in three postmortem series, two of sudden cardiac death and one of male violent death. There appears to be a critical level of severity of CAD which determines the risk of SD. The more severe the CAD within this range, the higher is the risk. Every annual cohort of SDs includes many patients with extremely severe, or 'burned-out', disease. CAD alone, however, is not a selective factor for sudden or not-sudden death. Epidemiological analysis (a) determines the age and frequency of persons reaching the critical risk level of CAD severity in the population (atherogenic factors); (b) influences the selection of the SD victims from the critical population (precipitating factors), and (c) determines the individual variation of CAD severity with which coronary heart disease and SD manifest themselves (sensitising and protecting factors)."}
{"id": "PMID:476736", "title": "Bifascicular block produced by hyperkalemia.", "content": "Bifascicular block is an unusual manifestation of hyperkalemia. A patient who developed right bundle branch block and left anterior hemiblock as the result of elevated serum potassium concentration is reported. After correction of his hyperkalemia, atrial fibrillation with a rapid ventricular response occurred. There was normal ventricular conduction despite this rate-related stress to his conduction system.", "contents": "Bifascicular block produced by hyperkalemia. Bifascicular block is an unusual manifestation of hyperkalemia. A patient who developed right bundle branch block and left anterior hemiblock as the result of elevated serum potassium concentration is reported. After correction of his hyperkalemia, atrial fibrillation with a rapid ventricular response occurred. There was normal ventricular conduction despite this rate-related stress to his conduction system."}
{"id": "PMID:476737", "title": "Reversibility of alcoholic cardiomyopathy with abstention from alcohol.", "content": "A 46-year-old chronic alcoholic patient with typical and severe congestive cardiomyopathy was studied hemodynamically on two separate occasions. The second study followed after a 1 1/2-year period of virtually complete abstention from alcohol and revealed that the left ventricular dysfunction had disappeared. Depsite persisting atrial fibrillation, the response to moderate exercise and to plasma volume expansion was within the normal range. When last seen, 29 months after initial hospitalization, the patient was symptom-free and was not given any treatment.", "contents": "Reversibility of alcoholic cardiomyopathy with abstention from alcohol. A 46-year-old chronic alcoholic patient with typical and severe congestive cardiomyopathy was studied hemodynamically on two separate occasions. The second study followed after a 1 1/2-year period of virtually complete abstention from alcohol and revealed that the left ventricular dysfunction had disappeared. Depsite persisting atrial fibrillation, the response to moderate exercise and to plasma volume expansion was within the normal range. When last seen, 29 months after initial hospitalization, the patient was symptom-free and was not given any treatment."}
{"id": "PMID:476738", "title": "Comparison of five different stress testing methods in the ECG diagnosis of coronary artery disease. Correlation with coronary arteriography.", "content": "Five different stress testing methods: bicycle ergometer exercise (BE), treadmill exercise (TD), isoproterenol infusion test (IPN), dopamine infusion test (DPM), and atrial pacing (AP), were performed on 90 male patients who underwent coronary arteriography. Ischemic S-T segment depression of 1.0 mm or greater was used as the criterion for a positive test. Within the group of 56 subjects having significant coronary artery disease (CAD) the diagnostic sensitivity of the single tests was as follows: 64.3% for BE, 66.1% for TD, 69.6% for IPN, 41.1% for DPM, 75.0% for AP. For the 34 subjects with no CAD the folowing specificity was found: 88.2% for BE and for TD, 82.3% for IPN, 85.3% for DPM, 63.8% for AP. When the results of the different tests were combined, it was seen that the association of an ergometric test with IPN enhanced the sensitivity of the exercise test (p less than 0.05) without significantly decreasing the specificity.", "contents": "Comparison of five different stress testing methods in the ECG diagnosis of coronary artery disease. Correlation with coronary arteriography. Five different stress testing methods: bicycle ergometer exercise (BE), treadmill exercise (TD), isoproterenol infusion test (IPN), dopamine infusion test (DPM), and atrial pacing (AP), were performed on 90 male patients who underwent coronary arteriography. Ischemic S-T segment depression of 1.0 mm or greater was used as the criterion for a positive test. Within the group of 56 subjects having significant coronary artery disease (CAD) the diagnostic sensitivity of the single tests was as follows: 64.3% for BE, 66.1% for TD, 69.6% for IPN, 41.1% for DPM, 75.0% for AP. For the 34 subjects with no CAD the folowing specificity was found: 88.2% for BE and for TD, 82.3% for IPN, 85.3% for DPM, 63.8% for AP. When the results of the different tests were combined, it was seen that the association of an ergometric test with IPN enhanced the sensitivity of the exercise test (p less than 0.05) without significantly decreasing the specificity."}
{"id": "PMID:476739", "title": "Afterload as a predeterminant of haemodynamics and segmental wall motion following coronary artery occlusion.", "content": "Maximal changes in haemodynamics and segmental wall motion were seen 2 min after coronary occlusion and were examined in relation to the loading conditions of the left ventricle before occlusion in 20 open chest dogs. There was a significant inverse relationship between the preligation mean aortic pressure and the percentage decrease in stroke volume following ligation. This relationship was observed whether afterload was distributed randomly (mean aortic pressure ranging from 9.7 to 17.6 kPa [73 to 132 mmHg]) between all dogs (r = 0.65; P less than 0.001) or altered by methoxamine (+4 kPa [+30 mmHg]) and nitroprusside (-3.2 kPa [-24 mmHg]) within the same dog (r = 0.82; P less than 0.001; n = 8). Although occlusion of the anterior descending artery caused a small (+5.5%) but significant increase in end-diastolic length of the non-ischaemic epicardial segment, the capacity for compensatory ventricular dilatation was not dependent on preligation afterload. However, the capacity of the ischaemic segment to undergo systolic expansion was significantly greater (+30.2% of end-systolic segment length) in those dogs with the lowest preligation MAP (8 to 12 kPa [60 to 90 mmHg]) compared with systolic lengthening of only 15.8% in the high afterload group (15 to 18 kPa [112 to 135 mmHg]). These data indicate that the loading conditions of the left ventricle predetermine the extent of global and segmental left ventricular dysfunction during the early phase of acute ischaemic injury.", "contents": "Afterload as a predeterminant of haemodynamics and segmental wall motion following coronary artery occlusion. Maximal changes in haemodynamics and segmental wall motion were seen 2 min after coronary occlusion and were examined in relation to the loading conditions of the left ventricle before occlusion in 20 open chest dogs. There was a significant inverse relationship between the preligation mean aortic pressure and the percentage decrease in stroke volume following ligation. This relationship was observed whether afterload was distributed randomly (mean aortic pressure ranging from 9.7 to 17.6 kPa [73 to 132 mmHg]) between all dogs (r = 0.65; P less than 0.001) or altered by methoxamine (+4 kPa [+30 mmHg]) and nitroprusside (-3.2 kPa [-24 mmHg]) within the same dog (r = 0.82; P less than 0.001; n = 8). Although occlusion of the anterior descending artery caused a small (+5.5%) but significant increase in end-diastolic length of the non-ischaemic epicardial segment, the capacity for compensatory ventricular dilatation was not dependent on preligation afterload. However, the capacity of the ischaemic segment to undergo systolic expansion was significantly greater (+30.2% of end-systolic segment length) in those dogs with the lowest preligation MAP (8 to 12 kPa [60 to 90 mmHg]) compared with systolic lengthening of only 15.8% in the high afterload group (15 to 18 kPa [112 to 135 mmHg]). These data indicate that the loading conditions of the left ventricle predetermine the extent of global and segmental left ventricular dysfunction during the early phase of acute ischaemic injury."}
{"id": "PMID:476740", "title": "Left ventricular dysfunction of isolated working rat hearts after chronic alcohol consumption.", "content": "The mechanical, haemodynamic, and energetic functions of isolated perfused working hearts from chronic alcoholic and control rats were studied. Male Long-Evans rats were fed a nutritionally-complete liquid diet containing 38% of daily calories as ethanol (isocaloric replacement with dextrin-maltose for controls) for 34 to 48 weeks. Alcoholics and controls received either 7.4% (low-fat) or 21.0% (high-fat) of calories from lipid. Animals were matched by dry heart weight for comparisons and hearts were evaluated at a constant heart rate in an improved isolated ejecting heart perfusion preparation. Ventricular function curves and the response to 10(-7) mol . litre-1 dobutamine HCl were obtained during normoxic and hypoxic perfusion. In normoxia, the hearts from the high- and low-fat alcoholic animals exhibited a significantly lower response of left ventricular maximum systolic pressure, left ventricular maximum rate of pressure rise and maximum ejection velocity to dobutamine than did the controls. In hypoxia, the left ventricular maximum systolic pressure of the low-fat alcoholic hearts was slightly, but significantly, lower than that of the low-fat control hearts at the three filling pressures studied. Also in hypoxia, the increase in left ventricular maximum rate of pressure rise with dobutamine was significantly less in the alcoholic hearts than in controls. These data suggest that long-term consumption of moderate amounts of alcohol even along with a nutritionally-adequate diet can result in depressed myocardial contractility and pump function that may be manifest when the heart is subjected to stress conditions.", "contents": "Left ventricular dysfunction of isolated working rat hearts after chronic alcohol consumption. The mechanical, haemodynamic, and energetic functions of isolated perfused working hearts from chronic alcoholic and control rats were studied. Male Long-Evans rats were fed a nutritionally-complete liquid diet containing 38% of daily calories as ethanol (isocaloric replacement with dextrin-maltose for controls) for 34 to 48 weeks. Alcoholics and controls received either 7.4% (low-fat) or 21.0% (high-fat) of calories from lipid. Animals were matched by dry heart weight for comparisons and hearts were evaluated at a constant heart rate in an improved isolated ejecting heart perfusion preparation. Ventricular function curves and the response to 10(-7) mol . litre-1 dobutamine HCl were obtained during normoxic and hypoxic perfusion. In normoxia, the hearts from the high- and low-fat alcoholic animals exhibited a significantly lower response of left ventricular maximum systolic pressure, left ventricular maximum rate of pressure rise and maximum ejection velocity to dobutamine than did the controls. In hypoxia, the left ventricular maximum systolic pressure of the low-fat alcoholic hearts was slightly, but significantly, lower than that of the low-fat control hearts at the three filling pressures studied. Also in hypoxia, the increase in left ventricular maximum rate of pressure rise with dobutamine was significantly less in the alcoholic hearts than in controls. These data suggest that long-term consumption of moderate amounts of alcohol even along with a nutritionally-adequate diet can result in depressed myocardial contractility and pump function that may be manifest when the heart is subjected to stress conditions."}
{"id": "PMID:476741", "title": "Factors determining delayed peak flow in canine myocardial reactive hyperaemia.", "content": "It has been suggested that delayed restoration of flow to the deeper layers of the myocardium is responsible for the gradual rise to peak flow rate characteristic of myocardial hyperaemia (RH). The distribution of coronary flow during RH was studied by injecting radiomicrospheres into the left atrium open chest dog preparations just prior to the end of a 15 s occlusion of the left circumflex branch. The artery was then reoccluded after 1, 2, or 4 s of RH. Myocardium perfused by the left anterior decending branch served as a control; the ratio of endocardial to epicardial (end/epi) perfusion in this bed averaged 1.14. During coronary occlusion and during the first second of RH the end/epi ratio in the hyperaemic zone averaged 50% of control, but rose progressively to equal control at the time of peak flow. Although evanescent subendocardial ischaemia may contribute to the gradual rise to peak flow rate it cannot by itself account for the time course of this response.", "contents": "Factors determining delayed peak flow in canine myocardial reactive hyperaemia. It has been suggested that delayed restoration of flow to the deeper layers of the myocardium is responsible for the gradual rise to peak flow rate characteristic of myocardial hyperaemia (RH). The distribution of coronary flow during RH was studied by injecting radiomicrospheres into the left atrium open chest dog preparations just prior to the end of a 15 s occlusion of the left circumflex branch. The artery was then reoccluded after 1, 2, or 4 s of RH. Myocardium perfused by the left anterior decending branch served as a control; the ratio of endocardial to epicardial (end/epi) perfusion in this bed averaged 1.14. During coronary occlusion and during the first second of RH the end/epi ratio in the hyperaemic zone averaged 50% of control, but rose progressively to equal control at the time of peak flow. Although evanescent subendocardial ischaemia may contribute to the gradual rise to peak flow rate it cannot by itself account for the time course of this response."}
{"id": "PMID:476742", "title": "Comparison of the arrhythmogenic effect of myocardial infarction in the cat and dog.", "content": "Myocardial infarction was produced in dogs and cats by occlusion of the left anterior descending coronary artery. Arrhythmia was present in dogs but not in cats 6 to 48 h after occlusion. The absence of arrhythmia in cats was not due to persistent myocardial depressant effects of anaesthesia administered during surgery. Studies in cats with surgically-induced heart block revealed multiple ventricular pacemakers but no change in average ventricular rate following coronary occlusion. These results suggest that sinus overdrive, although not elevated compared with the dog, is sufficient to suppress arrhythmia in the cat. Further, since small dogs developed significantly less arrhythmia than large dogs, heart size may be an additional factor in explaining the absence of arrhythmia in the cat.", "contents": "Comparison of the arrhythmogenic effect of myocardial infarction in the cat and dog. Myocardial infarction was produced in dogs and cats by occlusion of the left anterior descending coronary artery. Arrhythmia was present in dogs but not in cats 6 to 48 h after occlusion. The absence of arrhythmia in cats was not due to persistent myocardial depressant effects of anaesthesia administered during surgery. Studies in cats with surgically-induced heart block revealed multiple ventricular pacemakers but no change in average ventricular rate following coronary occlusion. These results suggest that sinus overdrive, although not elevated compared with the dog, is sufficient to suppress arrhythmia in the cat. Further, since small dogs developed significantly less arrhythmia than large dogs, heart size may be an additional factor in explaining the absence of arrhythmia in the cat."}
{"id": "PMID:476743", "title": "Augmentation of collateral blood flow to the ischaemic myocardium by oxygen inhalation following experimental coronary artery occlusion.", "content": "Since the mechanism by which oxygen reduces infarct size is not yet completely understood, we tested the hypothesis that breathing 100% oxygen may alter regional myocardial blood flow. In 23 anaesthetised open-chest dogs with acute occlusions of the left anterior descending coronary artery or of its apical branch, regional myocardial blood flow was determined by the microsphere technique at 15 and 30 min following coronary artery occlusion. Fifteen treated dogs were ventilated with 100% oxygen beginning 17 min after occlusion (ie, after the first determination of regional myocardial blood flow) and continuing until the end of the experiment, while the other 8 dogs served as controls and were ventilated continuously with room air. In treated dogs, following the administration of 100% oxygen, transmural flow at sites remote from the distribution of the occluded vessel (nonischaemic sites) decreased from 121 +/- 5 before oxygen to 108 +/- 6 cm3 . min-1 . 100g-1 (P less than 0.05). However, in the ischaemic sites it increased from 28 +/- 3 before oxygen to 32 +/- 3 cm3 . min-1 . 100g-1 (P less than 0.05). In the control dogs transmural flow, 15 and 30 min after occlusion, was, in nonischaemic sites, 118 +/- 7 and 125 +/- 10 cm3 . min-1 . 100g-1 NS, and in the ischaemic sites 27 +/- 4 and 25 +/- 3 cm3 . min-1 . 100g-1 NS, respectively. Thus, ventilation with 100% oxygen can augment perfusion of the ischaemic myocardium and this may be, in part, the mechanism by which it reduces infarct size.", "contents": "Augmentation of collateral blood flow to the ischaemic myocardium by oxygen inhalation following experimental coronary artery occlusion. Since the mechanism by which oxygen reduces infarct size is not yet completely understood, we tested the hypothesis that breathing 100% oxygen may alter regional myocardial blood flow. In 23 anaesthetised open-chest dogs with acute occlusions of the left anterior descending coronary artery or of its apical branch, regional myocardial blood flow was determined by the microsphere technique at 15 and 30 min following coronary artery occlusion. Fifteen treated dogs were ventilated with 100% oxygen beginning 17 min after occlusion (ie, after the first determination of regional myocardial blood flow) and continuing until the end of the experiment, while the other 8 dogs served as controls and were ventilated continuously with room air. In treated dogs, following the administration of 100% oxygen, transmural flow at sites remote from the distribution of the occluded vessel (nonischaemic sites) decreased from 121 +/- 5 before oxygen to 108 +/- 6 cm3 . min-1 . 100g-1 (P less than 0.05). However, in the ischaemic sites it increased from 28 +/- 3 before oxygen to 32 +/- 3 cm3 . min-1 . 100g-1 (P less than 0.05). In the control dogs transmural flow, 15 and 30 min after occlusion, was, in nonischaemic sites, 118 +/- 7 and 125 +/- 10 cm3 . min-1 . 100g-1 NS, and in the ischaemic sites 27 +/- 4 and 25 +/- 3 cm3 . min-1 . 100g-1 NS, respectively. Thus, ventilation with 100% oxygen can augment perfusion of the ischaemic myocardium and this may be, in part, the mechanism by which it reduces infarct size."}
{"id": "PMID:476744", "title": "Fibroblast function and the maintenance of the aortic-valve matrix.", "content": "The long-term behaviour of the aortic-valve allograft seems to be dependent on the maintenance of its matrix. Protein and collagen synthesis was studied in rat aortic-valves. Quantitative and qualitative methods proved the production of at least two protein pools. One protein pool is localised intracellularily with a 'turn-over' time of about 2 weeks, and a second pool is localised extracellularly with a 'turn-over' time of at least 8 weeks. The latter protein pool mainly consists of collagen.", "contents": "Fibroblast function and the maintenance of the aortic-valve matrix. The long-term behaviour of the aortic-valve allograft seems to be dependent on the maintenance of its matrix. Protein and collagen synthesis was studied in rat aortic-valves. Quantitative and qualitative methods proved the production of at least two protein pools. One protein pool is localised intracellularily with a 'turn-over' time of about 2 weeks, and a second pool is localised extracellularly with a 'turn-over' time of at least 8 weeks. The latter protein pool mainly consists of collagen."}
{"id": "PMID:476745", "title": "Effects of heparin and protamine on left ventricular performance in the dog.", "content": "The effects of protamine on left ventricular (LV) function were measured under conditions of controlled heart rate and proximal aortic pressure in eight anaesthetized, heparinised dogs. Protamine 3 mg.kg-1 produced a 21% decrease in LV dP/dt max, a 43% decrease in cardiac output, a 47% decrease in stroke work and decreases in systolic and diastolic pressures (-16%, -19% respectively). Protamine 6 mg.kg-1 resulted in a 17% decrease in LV dP/dt max, a 26% decrease in cardiac output, a 50% decrease in LV stroke work and 25 and 30% decreases in systolic and diastolic pressures. These results show that an impairment of LV function plays an important part in the circulatory depression produced by protamine.", "contents": "Effects of heparin and protamine on left ventricular performance in the dog. The effects of protamine on left ventricular (LV) function were measured under conditions of controlled heart rate and proximal aortic pressure in eight anaesthetized, heparinised dogs. Protamine 3 mg.kg-1 produced a 21% decrease in LV dP/dt max, a 43% decrease in cardiac output, a 47% decrease in stroke work and decreases in systolic and diastolic pressures (-16%, -19% respectively). Protamine 6 mg.kg-1 resulted in a 17% decrease in LV dP/dt max, a 26% decrease in cardiac output, a 50% decrease in LV stroke work and 25 and 30% decreases in systolic and diastolic pressures. These results show that an impairment of LV function plays an important part in the circulatory depression produced by protamine."}
{"id": "PMID:476746", "title": "Myocardial subcellular fractionation studies on cardiomyopathic Syrian hamsters.", "content": "Myocardial homogenates from control animals and from hamsters with hereditary cardiomyopathy were subjected to analytical subcellular fractionation and enzymic microanalysis. Animals without ventricular hypertrophy or overt heart failure were used in this study. The principal subcellular organelles were characterised by density gradient centrifugation. Apart from evidence of enhanced lysosomal and peroxisomal fragility, probably secondary to the intracellular oedema, the intracellular organelles investigated in this study were unaffected by the myopathic process. Highly significant increases in 5'-nucleotidase activity, a marker for the sarcolemma, and an increased equilibrium density of this organelle were found in the myopathic tissue. Ultrastructural studies revealed patchy myocytolysis associated with lysosomes and with more extensive invaginations of the sarcolemma. It is suggested that a primary defect in membrane composition, leading to increased cation permeability, is the underlying abnormality in the myopathic hamster.", "contents": "Myocardial subcellular fractionation studies on cardiomyopathic Syrian hamsters. Myocardial homogenates from control animals and from hamsters with hereditary cardiomyopathy were subjected to analytical subcellular fractionation and enzymic microanalysis. Animals without ventricular hypertrophy or overt heart failure were used in this study. The principal subcellular organelles were characterised by density gradient centrifugation. Apart from evidence of enhanced lysosomal and peroxisomal fragility, probably secondary to the intracellular oedema, the intracellular organelles investigated in this study were unaffected by the myopathic process. Highly significant increases in 5'-nucleotidase activity, a marker for the sarcolemma, and an increased equilibrium density of this organelle were found in the myopathic tissue. Ultrastructural studies revealed patchy myocytolysis associated with lysosomes and with more extensive invaginations of the sarcolemma. It is suggested that a primary defect in membrane composition, leading to increased cation permeability, is the underlying abnormality in the myopathic hamster."}
{"id": "PMID:476747", "title": "Role of angiotensin II in the development of peripheral vasoconstriction during cardiopulmonary bypass.", "content": "The possible relationship between angiotensin II -- the end product of the renin-angiotensin system, and the increase in peripheral vasoconstriction associated with cardiopulmonary bypass, has been investigated in 12 patients undergoing elective open-heart surgical procedures. Plasma angiotensin II (AII) levels were measured by radio-immunoassay at the start and the end of the period of cardiopulmonary bypass. Measurements of peripheral vascular resistance index were made at the times of AII sampling. Plasma AII levels rose during CPB from 78.0 to 231.3 pg.cm-3 (normal values less than 35 pg.cm-3). Peripheral vascular resistance index also rose during perfusion from 20.87 to 27.83 units. The rise in plasma AII levels and in peripheral vascular resistance index were correlated for each of the 12 patients. A highly significant correlation was obtained (r = 0.91; P less than 0.001). These results confirm the presence of parallel rises in plasma angiotensin II levels and in peripheral vasoconstriction during conventional non-pulsatile cardiopulmonary bypass, and suggest that increased plasma AII levels may be principal causative factor in the increased vasoconstriction associated with open-heart surgical procedures.", "contents": "Role of angiotensin II in the development of peripheral vasoconstriction during cardiopulmonary bypass. The possible relationship between angiotensin II -- the end product of the renin-angiotensin system, and the increase in peripheral vasoconstriction associated with cardiopulmonary bypass, has been investigated in 12 patients undergoing elective open-heart surgical procedures. Plasma angiotensin II (AII) levels were measured by radio-immunoassay at the start and the end of the period of cardiopulmonary bypass. Measurements of peripheral vascular resistance index were made at the times of AII sampling. Plasma AII levels rose during CPB from 78.0 to 231.3 pg.cm-3 (normal values less than 35 pg.cm-3). Peripheral vascular resistance index also rose during perfusion from 20.87 to 27.83 units. The rise in plasma AII levels and in peripheral vascular resistance index were correlated for each of the 12 patients. A highly significant correlation was obtained (r = 0.91; P less than 0.001). These results confirm the presence of parallel rises in plasma angiotensin II levels and in peripheral vasoconstriction during conventional non-pulsatile cardiopulmonary bypass, and suggest that increased plasma AII levels may be principal causative factor in the increased vasoconstriction associated with open-heart surgical procedures."}
{"id": "PMID:476748", "title": "Fallibility of the epicardial electrocardiogram in quantitation of myocardial necrosis.", "content": "Estimation of infarct size utilising the epicardial electrocardiogram has been the subject of considerable controversy. The present study re-examines this technique in a dog model. It demonstrates that epicardial Q waves and S-T elevation are qualitative markers of local necrosis with different degrees of reliability; neither, however, is quantitatively related in a simple linear fashion to extent of necrosis. Conclusions from previous studies have been influenced both by different mathematical analyses of data and the inclusions of nonhomogenous sites in linear regression models.", "contents": "Fallibility of the epicardial electrocardiogram in quantitation of myocardial necrosis. Estimation of infarct size utilising the epicardial electrocardiogram has been the subject of considerable controversy. The present study re-examines this technique in a dog model. It demonstrates that epicardial Q waves and S-T elevation are qualitative markers of local necrosis with different degrees of reliability; neither, however, is quantitatively related in a simple linear fashion to extent of necrosis. Conclusions from previous studies have been influenced both by different mathematical analyses of data and the inclusions of nonhomogenous sites in linear regression models."}
{"id": "PMID:476749", "title": "Electrophysiological actions of mexiletine on isolated rabbit atria and canine ventricular muscle and purkinje fibres.", "content": "The electrophysiological effects of varying concentrations of mexiletine on isolated rabbit sinus node and atrial preparations. Purkinje fibres, Purkinje-fibre-ventricular muscle junction and distal conduction system ('gate') were studied in oxygenated Tyrode solution at 35 degrees C using standard microelectrode techniques. 'Therapeutic' concentrations of mexiletine (0.5 to 2.0 micrograms.cm-3) had little effect on the atrial action potentials or on sinus node automaticity but sino-atrial conduction was delayed. In Purkinje fibres, these concentrations of the drug depressed the dV/dtmax of phase 0, conduction velocity and membrane responsiveness accompanied by significant shortening of the action potential duration and the effective refractory period, the change in the former always being greater than that in the latter. Mexiletine decreased antegrade as well as retrograde conduction at the Purkinje-fibre-ventricular muscle junction, while producing a uniform shortening of the action potential duration in the distal conduction system without a preferential effect at the 'gate'. The mechanisms of antiarrhythmic actions of mexiletine are thus complex but resemble those of lignocaine in superfused isolated cardiac muscle.", "contents": "Electrophysiological actions of mexiletine on isolated rabbit atria and canine ventricular muscle and purkinje fibres. The electrophysiological effects of varying concentrations of mexiletine on isolated rabbit sinus node and atrial preparations. Purkinje fibres, Purkinje-fibre-ventricular muscle junction and distal conduction system ('gate') were studied in oxygenated Tyrode solution at 35 degrees C using standard microelectrode techniques. 'Therapeutic' concentrations of mexiletine (0.5 to 2.0 micrograms.cm-3) had little effect on the atrial action potentials or on sinus node automaticity but sino-atrial conduction was delayed. In Purkinje fibres, these concentrations of the drug depressed the dV/dtmax of phase 0, conduction velocity and membrane responsiveness accompanied by significant shortening of the action potential duration and the effective refractory period, the change in the former always being greater than that in the latter. Mexiletine decreased antegrade as well as retrograde conduction at the Purkinje-fibre-ventricular muscle junction, while producing a uniform shortening of the action potential duration in the distal conduction system without a preferential effect at the 'gate'. The mechanisms of antiarrhythmic actions of mexiletine are thus complex but resemble those of lignocaine in superfused isolated cardiac muscle."}
{"id": "PMID:476750", "title": "Extracellular potassium activity changes in the canine myocardium after acute coronary occlusion and the influence of beta-blockade.", "content": "Extracellular potassium activity before and after coronary occlusion was measured in the canine heart by means of potassium-selective surface electrodes. In the ischaemic myocardium potassium activity rapidly increased from a preocclusion value of 3.2 +/- 0.3 mmol.litre-1 to 10 +/- 0.6 mmol.litre-1 within the first 5 min and to about 11.3 +/- 0.5 mmol.litre-1 after 10 min of ischaemia (range from 9.5 to 14.5 mmol.litre-1). Following the initial 10 min of ischaemia no further increase was measured. In the non-ischaemic area potassium activity remained constant. Acute beta-blockade significantly attenuated the initial rate of increase in potassium activity; however, beta-blockade did not influence maximal values of extracellular potassium activity measured after occlusion. Lowering of heart rate by vagal stimulation did not modify the pattern of increase in potassium during acute ischaemia. Following ventricular fibrillation, a slow but continuous rise in potassium activity was found. These results demonstrate that extracellular potassium activity in the acutely ischaemic myocardium is considerably higher than indicated by the technique of coronary vein sampling and is in the range necessary for the development of re-entrant arrhythmias in the early phase after coronary occlusion.", "contents": "Extracellular potassium activity changes in the canine myocardium after acute coronary occlusion and the influence of beta-blockade. Extracellular potassium activity before and after coronary occlusion was measured in the canine heart by means of potassium-selective surface electrodes. In the ischaemic myocardium potassium activity rapidly increased from a preocclusion value of 3.2 +/- 0.3 mmol.litre-1 to 10 +/- 0.6 mmol.litre-1 within the first 5 min and to about 11.3 +/- 0.5 mmol.litre-1 after 10 min of ischaemia (range from 9.5 to 14.5 mmol.litre-1). Following the initial 10 min of ischaemia no further increase was measured. In the non-ischaemic area potassium activity remained constant. Acute beta-blockade significantly attenuated the initial rate of increase in potassium activity; however, beta-blockade did not influence maximal values of extracellular potassium activity measured after occlusion. Lowering of heart rate by vagal stimulation did not modify the pattern of increase in potassium during acute ischaemia. Following ventricular fibrillation, a slow but continuous rise in potassium activity was found. These results demonstrate that extracellular potassium activity in the acutely ischaemic myocardium is considerably higher than indicated by the technique of coronary vein sampling and is in the range necessary for the development of re-entrant arrhythmias in the early phase after coronary occlusion."}
{"id": "PMID:476751", "title": "Effect of mexiletine on monophasic action potentials recorded from the right ventricle in man.", "content": "The effect of mexiletine, a new antiarrhythmic agent, on ventricular refractoriness and monophasic action potentials recorded from the right ventricle was studied in nine subjects. The effective refractory period of the right ventricle was determined by the extra stimulus technique using a pacing electrode situated at the right ventricular apex. Following this determination the right ventricular apex was paced at a constant cycle length and premature stimuli were introduced starting at a coupling interval of 2 ms greater than the ventricular refractory period and then at progressively increasing coupling intervals of 5 ms increments. Simultaneous recordings of monophasic action potentials of both the regular paced beats and the induced premature beats were made using a  specially designed suction electrode catheter. The monophasic action potential durations were measured at 50% and 90% repolarisation. All these control measurements were repeated after an intravenous dose of 2 mg.kg-1 body wt. of mexiletine. The results showed that mexiletine did not significantly change the effective ventricular refractory period nor did it alter the monophasic action potential duration of the regular paced beat. The drug did, however, significantly prolong the monophasic action potential duration of the early induced premature beats and it is possible that this property of the drug may be related to its antiarrhythmic activity.", "contents": "Effect of mexiletine on monophasic action potentials recorded from the right ventricle in man. The effect of mexiletine, a new antiarrhythmic agent, on ventricular refractoriness and monophasic action potentials recorded from the right ventricle was studied in nine subjects. The effective refractory period of the right ventricle was determined by the extra stimulus technique using a pacing electrode situated at the right ventricular apex. Following this determination the right ventricular apex was paced at a constant cycle length and premature stimuli were introduced starting at a coupling interval of 2 ms greater than the ventricular refractory period and then at progressively increasing coupling intervals of 5 ms increments. Simultaneous recordings of monophasic action potentials of both the regular paced beats and the induced premature beats were made using a  specially designed suction electrode catheter. The monophasic action potential durations were measured at 50% and 90% repolarisation. All these control measurements were repeated after an intravenous dose of 2 mg.kg-1 body wt. of mexiletine. The results showed that mexiletine did not significantly change the effective ventricular refractory period nor did it alter the monophasic action potential duration of the regular paced beat. The drug did, however, significantly prolong the monophasic action potential duration of the early induced premature beats and it is possible that this property of the drug may be related to its antiarrhythmic activity."}
{"id": "PMID:476752", "title": "Effect of mexiletine on conduction of premature ventricular beats in man: a study using monophasic action potential recordings from the right ventricle.", "content": "Ventricular conduction intervals between a stimulating pacemaker electrode at the right ventricular apex and an impulse detecting monophasic action potential electrode at the right ventricular septum were measured in nine subjects. The right ventricle was paced at a constant rate and programmed premature ventricular stimuli were introduced after every eighth paced beat beginning at the refractory period. The ventricular conduction intervals were measured from the pacemaker artefact to the onset of depolarisation of the MAP recording. In the control recordings a short period of prolonged (subnormal) conduction lasting for 5 to 26 ms after the refractory period was present in six of the nine subjects. In eight subjects a period of 'supernormal' conduction lasting from 50 to 180 ms was present. The effect of mexiletine was to increase the subnormal conduction and to abolish the supernormal conduction. It is concluded that a small degree of supernormal conduction occurs in the normal human ventricle possibly as a result of phase 4 depolarisation of cells of the specialised conducting system.", "contents": "Effect of mexiletine on conduction of premature ventricular beats in man: a study using monophasic action potential recordings from the right ventricle. Ventricular conduction intervals between a stimulating pacemaker electrode at the right ventricular apex and an impulse detecting monophasic action potential electrode at the right ventricular septum were measured in nine subjects. The right ventricle was paced at a constant rate and programmed premature ventricular stimuli were introduced after every eighth paced beat beginning at the refractory period. The ventricular conduction intervals were measured from the pacemaker artefact to the onset of depolarisation of the MAP recording. In the control recordings a short period of prolonged (subnormal) conduction lasting for 5 to 26 ms after the refractory period was present in six of the nine subjects. In eight subjects a period of 'supernormal' conduction lasting from 50 to 180 ms was present. The effect of mexiletine was to increase the subnormal conduction and to abolish the supernormal conduction. It is concluded that a small degree of supernormal conduction occurs in the normal human ventricle possibly as a result of phase 4 depolarisation of cells of the specialised conducting system."}
{"id": "PMID:476753", "title": "Coupling interval, exit block, and periodicity of ventricular parasystolic rhythm.", "content": "The electrocardiograms in this study of parasystolic rhythm were tape recorded and then analysed with a special purpose hybrid computer. The rate of appearance of specific inter-ectopic intervals was shown to change significantly with small changes in either the sinus or parasystolic pacemaker period. Natural changes in the period of the ectopic pacemakers were observed over several hours. Changes amounting to 14% within 10 min and 18% overall were observed in one patient, and a gradual lengthening of 10% over 3 h in another. A previously undescribed form of exit block has been discovered in one patient where the block remained active for a given time only after propagation from the parasystolic focus. Different interectopic intervals were shown to contribute to specific and restricted coupling interval locations in diastole. Hence this form of exit block, by preventing some inter-ectopic intervals from propagating, limited the locations in diastole in which parasystolic ectopic complexes could appear. In this case later diastolic complexes were inhibited and hence fusion complexes were completely absent. Parasystolic rhythm, with and without the exit block described, was simulated successfully by a digital computer. The simulations aided our understanding of the clinical data.", "contents": "Coupling interval, exit block, and periodicity of ventricular parasystolic rhythm. The electrocardiograms in this study of parasystolic rhythm were tape recorded and then analysed with a special purpose hybrid computer. The rate of appearance of specific inter-ectopic intervals was shown to change significantly with small changes in either the sinus or parasystolic pacemaker period. Natural changes in the period of the ectopic pacemakers were observed over several hours. Changes amounting to 14% within 10 min and 18% overall were observed in one patient, and a gradual lengthening of 10% over 3 h in another. A previously undescribed form of exit block has been discovered in one patient where the block remained active for a given time only after propagation from the parasystolic focus. Different interectopic intervals were shown to contribute to specific and restricted coupling interval locations in diastole. Hence this form of exit block, by preventing some inter-ectopic intervals from propagating, limited the locations in diastole in which parasystolic ectopic complexes could appear. In this case later diastolic complexes were inhibited and hence fusion complexes were completely absent. Parasystolic rhythm, with and without the exit block described, was simulated successfully by a digital computer. The simulations aided our understanding of the clinical data."}
{"id": "PMID:476755", "title": "Release of alpha hydroxybutyrate from neonatal rat heart cell cultures exposed to anoxia and reoxygenation: comparison with impairment of structure and function of damaged cardiac cells.", "content": "Spontaneously beating monolayer cultures of neonatal rat heart cells first exposed to depletion of oxygen and metabolic substrates for 1 to 7 h (anoxia). Subsequently the cultures were resupplied with oxygen and substrates (reoxygenation). The release of alpha-hydroxybutyrate dehydrogenase (HBDH) from the cells, the extent of necrosis, and the changes in spontaneous contractile activity were measured. HBDH release was observed to start after 1 h of anoxia and to increase to 84% of intracellular HBDH activity after 7 h of anoxia. Reoxygenation of anoxic heart cells is associated with accelerated HBDH release (oxygen paradox). The activity of HBDH released by cardiac cells, the number of necrotic cells and the impairment of beating capacity of the cells depend on the duration of the anoxic period in a similar way. This study demonstrates that cardiac cell death can be assessed quantitatively and reliably by the measurement of the activity of HBDH released by the cells.", "contents": "Release of alpha hydroxybutyrate from neonatal rat heart cell cultures exposed to anoxia and reoxygenation: comparison with impairment of structure and function of damaged cardiac cells. Spontaneously beating monolayer cultures of neonatal rat heart cells first exposed to depletion of oxygen and metabolic substrates for 1 to 7 h (anoxia). Subsequently the cultures were resupplied with oxygen and substrates (reoxygenation). The release of alpha-hydroxybutyrate dehydrogenase (HBDH) from the cells, the extent of necrosis, and the changes in spontaneous contractile activity were measured. HBDH release was observed to start after 1 h of anoxia and to increase to 84% of intracellular HBDH activity after 7 h of anoxia. Reoxygenation of anoxic heart cells is associated with accelerated HBDH release (oxygen paradox). The activity of HBDH released by cardiac cells, the number of necrotic cells and the impairment of beating capacity of the cells depend on the duration of the anoxic period in a similar way. This study demonstrates that cardiac cell death can be assessed quantitatively and reliably by the measurement of the activity of HBDH released by the cells."}
{"id": "PMID:476756", "title": "Electrophysiological effects of ethmozin on canine myocardium.", "content": "The electrophysiological effects of ethmozin on canine myocardium were studied in anesthetised open-chest animals and in superfused Purkinje fibres. The drug did not change spontaneous sinus node cycle length and atrioventricular conduction time when selectively injected into the sinus nodal artery and into the posterior septal artery, respectively. Right and left ventricular diastolic excitability threshold and refractory period were increased following the intravenous administration of ethmozin, 4 mg/kg-1. Ethmozin (3 to 5 mg/kg-1 i.v.) markedly increased conduction delay in the ischaemic zone of the left ventricle during acute coronary artery occlusion. This change was associated with the development of ventricular fibrillation in 50% of the dogs. In vitro, ethmozin 1 X 10(-3) and 1 X 10(-2) g.litre-1 shortened Purkinje fibres' action potential duration. At 1 X 10(-2) g.litre-1, ethmozin decreased the rate of rise of phase 0, and slightly reduced action potential amplitude.", "contents": "Electrophysiological effects of ethmozin on canine myocardium. The electrophysiological effects of ethmozin on canine myocardium were studied in anesthetised open-chest animals and in superfused Purkinje fibres. The drug did not change spontaneous sinus node cycle length and atrioventricular conduction time when selectively injected into the sinus nodal artery and into the posterior septal artery, respectively. Right and left ventricular diastolic excitability threshold and refractory period were increased following the intravenous administration of ethmozin, 4 mg/kg-1. Ethmozin (3 to 5 mg/kg-1 i.v.) markedly increased conduction delay in the ischaemic zone of the left ventricle during acute coronary artery occlusion. This change was associated with the development of ventricular fibrillation in 50% of the dogs. In vitro, ethmozin 1 X 10(-3) and 1 X 10(-2) g.litre-1 shortened Purkinje fibres' action potential duration. At 1 X 10(-2) g.litre-1, ethmozin decreased the rate of rise of phase 0, and slightly reduced action potential amplitude."}
{"id": "PMID:476772", "title": "Changes in growth kinetics of jejunal epithelium in mice maintained on an elemental diet.", "content": "Changes in the kinetics of the intestinal epithelium were observed in mice maintained on an elemental diet containing hydrolysed protein and medium chain triglycerides. An increase in the length of the villi seen shortly after commencement of the diet was followed by a reduction in the rate of proliferation in the crypt. After 7 days on the diet, an equilibrium state was reached with the cellularity of the villi being 120% that of control while the number of proliferative cells/crypt was reduced by 35%. The proliferative response of the crypt following irradiation occurred 16 hr later in diet-fed mice than in controls. It was postulated that, because of the increased cellularity of the villus compartment in diet-fed mice, additional time was required to reduce the number of villus cells to a critical level at which a proliferative response is induced in the crypt.", "contents": "Changes in growth kinetics of jejunal epithelium in mice maintained on an elemental diet. Changes in the kinetics of the intestinal epithelium were observed in mice maintained on an elemental diet containing hydrolysed protein and medium chain triglycerides. An increase in the length of the villi seen shortly after commencement of the diet was followed by a reduction in the rate of proliferation in the crypt. After 7 days on the diet, an equilibrium state was reached with the cellularity of the villi being 120% that of control while the number of proliferative cells/crypt was reduced by 35%. The proliferative response of the crypt following irradiation occurred 16 hr later in diet-fed mice than in controls. It was postulated that, because of the increased cellularity of the villus compartment in diet-fed mice, additional time was required to reduce the number of villus cells to a critical level at which a proliferative response is induced in the crypt."}
{"id": "PMID:476773", "title": "Estimation of effective eosinopoiesis and bone marrow eosinophil reserve capacity in normal man.", "content": "The eosinophil reserve capacity of the post-mitotic granulocyte compartment in the bone marrow and the effective eosinopoiesis in three haematologically normal men have been quantified by means of kinetic parameters of [3H]thymidine flash-labelled peripheral blood eosinophils. From (a) the time of the appearance in the blood of labelled eosinophils after the tracer injection, (b) the inflow characteristics of the labelled eosinophils in the blood and (c) the magnitude of the eosinophil granulocyte pool in the venous blood, the effective eosinopoiesis (i.e. the eosinophil turnover) was calculated to range between 0.014 and 0.031 x 10(9) cells/kg body weight per day (mean 0.22 x 10(9) cell/kg per day). The post-mitotic eosinophil reserve capacity of the bone marrow ranged from 0.09 to 0.20 x 10(9) cells/kg body weight (mean 0.14 x 10(9) cells/kg). The large reserve pool and the high turnover rate may contribute to sudden rises of the peripheral blood oesinophil counts in some cases of eosinophilia.", "contents": "Estimation of effective eosinopoiesis and bone marrow eosinophil reserve capacity in normal man. The eosinophil reserve capacity of the post-mitotic granulocyte compartment in the bone marrow and the effective eosinopoiesis in three haematologically normal men have been quantified by means of kinetic parameters of [3H]thymidine flash-labelled peripheral blood eosinophils. From (a) the time of the appearance in the blood of labelled eosinophils after the tracer injection, (b) the inflow characteristics of the labelled eosinophils in the blood and (c) the magnitude of the eosinophil granulocyte pool in the venous blood, the effective eosinopoiesis (i.e. the eosinophil turnover) was calculated to range between 0.014 and 0.031 x 10(9) cells/kg body weight per day (mean 0.22 x 10(9) cell/kg per day). The post-mitotic eosinophil reserve capacity of the bone marrow ranged from 0.09 to 0.20 x 10(9) cells/kg body weight (mean 0.14 x 10(9) cells/kg). The large reserve pool and the high turnover rate may contribute to sudden rises of the peripheral blood oesinophil counts in some cases of eosinophilia."}
{"id": "PMID:476774", "title": "Further studies on mobilization of CFUs.", "content": "Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after i.v. injection. Some variation in activity could not be excluded. As with Salmonella typhosa LPS, zymosan gave an increase in circulating CFUs during the first few hr and a second peak a few days later. After injection of zymosan as well as S. typhosa LPS the second peak in the blood was accompanied by a large increase in CFUs numbers in the spleen. PHA gave an immediate mobilization of CFUs, but the mobilization after injection of Con A during the first few hr occurred more slowly. After injection of S. typhosa LPS, zymosan and PHA the blood C3 level was found to be depressed considerably. This might indicate that the complement system is involved in the early mobilization of CFUs. Dexamethasone, a synthetic hormone which has been reported to give sequestration of several cell types in the bone marrow, did not inhibit the early and late mobilization of CFUs which normally occurs after injection of S. typhosa LPS.", "contents": "Further studies on mobilization of CFUs. Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after i.v. injection. Some variation in activity could not be excluded. As with Salmonella typhosa LPS, zymosan gave an increase in circulating CFUs during the first few hr and a second peak a few days later. After injection of zymosan as well as S. typhosa LPS the second peak in the blood was accompanied by a large increase in CFUs numbers in the spleen. PHA gave an immediate mobilization of CFUs, but the mobilization after injection of Con A during the first few hr occurred more slowly. After injection of S. typhosa LPS, zymosan and PHA the blood C3 level was found to be depressed considerably. This might indicate that the complement system is involved in the early mobilization of CFUs. Dexamethasone, a synthetic hormone which has been reported to give sequestration of several cell types in the bone marrow, did not inhibit the early and late mobilization of CFUs which normally occurs after injection of S. typhosa LPS."}
{"id": "PMID:476775", "title": "The effect of temperature on the turnover of taste bud cells in catfish.", "content": "Renewal of taste bud cells on the barbels of channel catfish was studied. Groups of catfish, held in and acclimitized to 14 degrees C, 18 degrees C, 22 degrees C and 30 degrees C dechlorinated tap water were injected with [3H]thymidine (3.0 muCi/g body weight intraperitoneally). Barbels were sampled at various times after injection and prepared for light microscope autoradiography. Results show that epithelial cells surrounding the taste buds divide and some of their daughter cells migrate into the taste buds. The time at which 50% of the labelled cells have degenerated is taken as the average turnover time or average life span of the taste bud cells. The average life span as well as the time spent inside the taste buds is highly temperature-dependent. At 14 degrees C, 18 degrees C, 22 degrees C and 30 degrees C the average life span is on the order of 40, 30, 15 and 12 days respectively. Further studies indicate that both light and dark staining cells of the taste bud were labelled.", "contents": "The effect of temperature on the turnover of taste bud cells in catfish. Renewal of taste bud cells on the barbels of channel catfish was studied. Groups of catfish, held in and acclimitized to 14 degrees C, 18 degrees C, 22 degrees C and 30 degrees C dechlorinated tap water were injected with [3H]thymidine (3.0 muCi/g body weight intraperitoneally). Barbels were sampled at various times after injection and prepared for light microscope autoradiography. Results show that epithelial cells surrounding the taste buds divide and some of their daughter cells migrate into the taste buds. The time at which 50% of the labelled cells have degenerated is taken as the average turnover time or average life span of the taste bud cells. The average life span as well as the time spent inside the taste buds is highly temperature-dependent. At 14 degrees C, 18 degrees C, 22 degrees C and 30 degrees C the average life span is on the order of 40, 30, 15 and 12 days respectively. Further studies indicate that both light and dark staining cells of the taste bud were labelled."}
{"id": "PMID:476776", "title": "A cell kinetic model to explain the time of appearance of skin reaction after X-rays or ultraviolet light irradiation.", "content": "Skin reactions to various doses of X-rays (300 and 10 kV) and ultraviolet light (u.v.) have been compared using hairless mice. Two regions of epidermis with widely differing cell kinetics and gross structure have been compared. Little evidence could be found to support the idea that the early phases of the reaction are dependent on cell cycle time. The data can be explained by a model based on the assumption that epidermis contains only a small fraction of clonogenic (stem) cells and this fraction may vary in different epidermal regions. X-rays appear to exert their greatest destructive action on these clonogenic cells while u.v. is more indiscriminate in its action, killing both clonogenic and non-clonogenic cells.", "contents": "A cell kinetic model to explain the time of appearance of skin reaction after X-rays or ultraviolet light irradiation. Skin reactions to various doses of X-rays (300 and 10 kV) and ultraviolet light (u.v.) have been compared using hairless mice. Two regions of epidermis with widely differing cell kinetics and gross structure have been compared. Little evidence could be found to support the idea that the early phases of the reaction are dependent on cell cycle time. The data can be explained by a model based on the assumption that epidermis contains only a small fraction of clonogenic (stem) cells and this fraction may vary in different epidermal regions. X-rays appear to exert their greatest destructive action on these clonogenic cells while u.v. is more indiscriminate in its action, killing both clonogenic and non-clonogenic cells."}
{"id": "PMID:476777", "title": "The enhancement of haemopoietic stem cell recovery in irradiated mice by prior treatment with cyclophosphamide.", "content": "Studies are reported of the enhancement of stem cell recovery following whole body irradiation as a result of prior administration of cyclophosphamide. It is shown that the much larger enhancement of regeneration observed for the hosts own surviving stem cells, compared to the regeneration of injected bone marrow stem cells, is due to the different numbers of stem cells initiating the regeneration in conjunction with the time course of stem cell regeneration. The results show that the environmental changes produced by cyclophosphmide greatly enhance haemopoietic recovery even though at the dose used this agent is relatively toxic to stem cells. Furthermore it has been shown that the level of stem cell regeneration is nearly independent of the gamma-ray dose in the range 3-8 gray (300-800 rad). If human bone marrow should respond similarly it follows that regeneration produced by cytotoxic drugs administered prior to radiation embodies a considerable safety factor as far as recovery of the haemopoietic system is concerned.", "contents": "The enhancement of haemopoietic stem cell recovery in irradiated mice by prior treatment with cyclophosphamide. Studies are reported of the enhancement of stem cell recovery following whole body irradiation as a result of prior administration of cyclophosphamide. It is shown that the much larger enhancement of regeneration observed for the hosts own surviving stem cells, compared to the regeneration of injected bone marrow stem cells, is due to the different numbers of stem cells initiating the regeneration in conjunction with the time course of stem cell regeneration. The results show that the environmental changes produced by cyclophosphmide greatly enhance haemopoietic recovery even though at the dose used this agent is relatively toxic to stem cells. Furthermore it has been shown that the level of stem cell regeneration is nearly independent of the gamma-ray dose in the range 3-8 gray (300-800 rad). If human bone marrow should respond similarly it follows that regeneration produced by cytotoxic drugs administered prior to radiation embodies a considerable safety factor as far as recovery of the haemopoietic system is concerned."}
{"id": "PMID:476779", "title": "Circadian rhythms in mouse epidermal basal cell proliferation. Variations in compartment size, flux and phase duration.", "content": "Several kinetic parameters of basal cell proliferation in hairless mouse epidermis were studied, and all parameters clearly showed circadian fluctuations during two successive 24 hr periods. Mitotic indices and the mitotic rate were studied in histological sections; the proportions of cells with S and G2 phase DNA content were measured by flow cytometry of isolated basal cells, and the [3H]TdR labelling indices and grain densities were determined by autoradiography in smears from basal cell suspensions. The influx and efflux of cells from each cell cycle phase were calculated from sinusoidal curves adapted to the cell kinetic findings and the phase durations were determined. A peak of cells in S phase was observed around midnight, and a cohort of partially synchronized cells passed from the S phase to the G2 phase and traversed the G2 phase and mitosis in the early morning. The fluctuations in the influx of cells into the S phase were small compared with the variations in efflux from the S phase and the flux through the subsequent cell cycle phases. The resulting delay in cell cycle traverse through S phase before midnight could well account for the accumulation of cells in S phase and, therefore, also the subsequent partial synchrony of cell cycle traverse through the G2 phase and mitosis. Circadian variations in the duration of the S phase, the G2 phase and mitosis were clearly demonstrated.", "contents": "Circadian rhythms in mouse epidermal basal cell proliferation. Variations in compartment size, flux and phase duration. Several kinetic parameters of basal cell proliferation in hairless mouse epidermis were studied, and all parameters clearly showed circadian fluctuations during two successive 24 hr periods. Mitotic indices and the mitotic rate were studied in histological sections; the proportions of cells with S and G2 phase DNA content were measured by flow cytometry of isolated basal cells, and the [3H]TdR labelling indices and grain densities were determined by autoradiography in smears from basal cell suspensions. The influx and efflux of cells from each cell cycle phase were calculated from sinusoidal curves adapted to the cell kinetic findings and the phase durations were determined. A peak of cells in S phase was observed around midnight, and a cohort of partially synchronized cells passed from the S phase to the G2 phase and traversed the G2 phase and mitosis in the early morning. The fluctuations in the influx of cells into the S phase were small compared with the variations in efflux from the S phase and the flux through the subsequent cell cycle phases. The resulting delay in cell cycle traverse through S phase before midnight could well account for the accumulation of cells in S phase and, therefore, also the subsequent partial synchrony of cell cycle traverse through the G2 phase and mitosis. Circadian variations in the duration of the S phase, the G2 phase and mitosis were clearly demonstrated."}
{"id": "PMID:476778", "title": "Studies on the mechanism of haemopoietic stem cell (CFUs) mobilization. A role of the complement system.", "content": "A variety of substances can mobilize haemopoietic stem cells (CFUs) into the peripheral blood. In this study the involvement of the complement system in the mobilization process was investigated. Pretreatment of mice with the complement-activating factor of cobra venom (CoF), which lowered the serum C3 levels to 10-25% of the normal value, could completely prevent CFUs mobilization induced by high doses of CoF, endotoxin (ET) from Salmonella typhosa, inulin, zymosan and the proteolytic enzymes proteinase and trypsin. On the other hand, mobilization induced by the polyanions dextran sulphate and the copolymer of polymethacrylic acid and styrene could not be prevented, or at least affected only slightly. There appears to be a relationship between the extent of decomplementation by CoF and the extent of CFUs mobilization induced by ET. The results indicate that certain agents mobilize CFUs via the complement system, whereas other agents induce CFUs mobilization independent of the availability of complement components.", "contents": "Studies on the mechanism of haemopoietic stem cell (CFUs) mobilization. A role of the complement system. A variety of substances can mobilize haemopoietic stem cells (CFUs) into the peripheral blood. In this study the involvement of the complement system in the mobilization process was investigated. Pretreatment of mice with the complement-activating factor of cobra venom (CoF), which lowered the serum C3 levels to 10-25% of the normal value, could completely prevent CFUs mobilization induced by high doses of CoF, endotoxin (ET) from Salmonella typhosa, inulin, zymosan and the proteolytic enzymes proteinase and trypsin. On the other hand, mobilization induced by the polyanions dextran sulphate and the copolymer of polymethacrylic acid and styrene could not be prevented, or at least affected only slightly. There appears to be a relationship between the extent of decomplementation by CoF and the extent of CFUs mobilization induced by ET. The results indicate that certain agents mobilize CFUs via the complement system, whereas other agents induce CFUs mobilization independent of the availability of complement components."}
{"id": "PMID:476780", "title": "Non-random association of mitotic cells from cultured human lymphocyte suspensions.", "content": "By using chromosome markers in two separate cell lines of a human dispermic chimera, it was shown that 4.9% of metaphases in suspensions of PHA-cultured lymphocytes were paired because of non-random factors. A similar amount of metaphase pairing occurred in cultured lymphocytes of normal donors, and evidence from the relative mitotic cycles of the paired cells indicated that some of this pairing was non-random. Such non-random pairing could be a source of bias in cell kinetic and other studies involving metaphase cells in lymphocyte cultures.", "contents": "Non-random association of mitotic cells from cultured human lymphocyte suspensions. By using chromosome markers in two separate cell lines of a human dispermic chimera, it was shown that 4.9% of metaphases in suspensions of PHA-cultured lymphocytes were paired because of non-random factors. A similar amount of metaphase pairing occurred in cultured lymphocytes of normal donors, and evidence from the relative mitotic cycles of the paired cells indicated that some of this pairing was non-random. Such non-random pairing could be a source of bias in cell kinetic and other studies involving metaphase cells in lymphocyte cultures."}
{"id": "PMID:476781", "title": "Transit times through the cycle phases of jejunal crypt cells of the mouse. Analysis in terms of the mean values and the variances.", "content": "Mean transit times as well as variances of the transit times through the individual phases of the cell cycle have been determined for the crypt epithelial cells of the jejunum of the mouse. To achieve this the fraction of labelled mitoses (FLM) technique has been modified by double labelling with [3H] and [14C]thymidine. Mice were given a first injection of [3H]thymidine, and 2 hr later a second injection of [14C]thymidine. This produces a narrow subpopulation of purely 3H-labelled cells at the beginning of G2-phase and a corresponding subpopulation of purely 14C-labelled cells at the beginning of the S-phase. When these two subpopulations progress through the cell cycle, one obtains FLM waves of purely 3H- and purely 14C-labelled mitoses. These waves have considerably better resolution than the conventional FLM-curves. From the temporal positions of the observed maxima the mean transit times of the cells through the individual phases of the cycle can be determined. Moreover one obtains from the width of the individual waves the variances of the transit times through the individual phases. It has been found, that the variances of the transit times through successive phases are additive. This indicates that the transit times of cells through successive phases are independently distributed. This statistical independence is an implicit assumption in most of the models applied to the analysis of FLM curves, however there had previously been no experimental support of this assumption. A further result is, that the variance of the transit time through any phase of the cycle is proportional to the mean transit time. This implies that the progress of the crypt epithelial cells is subject to an equal degree of randomness in the various phases of the cycle.", "contents": "Transit times through the cycle phases of jejunal crypt cells of the mouse. Analysis in terms of the mean values and the variances. Mean transit times as well as variances of the transit times through the individual phases of the cell cycle have been determined for the crypt epithelial cells of the jejunum of the mouse. To achieve this the fraction of labelled mitoses (FLM) technique has been modified by double labelling with [3H] and [14C]thymidine. Mice were given a first injection of [3H]thymidine, and 2 hr later a second injection of [14C]thymidine. This produces a narrow subpopulation of purely 3H-labelled cells at the beginning of G2-phase and a corresponding subpopulation of purely 14C-labelled cells at the beginning of the S-phase. When these two subpopulations progress through the cell cycle, one obtains FLM waves of purely 3H- and purely 14C-labelled mitoses. These waves have considerably better resolution than the conventional FLM-curves. From the temporal positions of the observed maxima the mean transit times of the cells through the individual phases of the cycle can be determined. Moreover one obtains from the width of the individual waves the variances of the transit times through the individual phases. It has been found, that the variances of the transit times through successive phases are additive. This indicates that the transit times of cells through successive phases are independently distributed. This statistical independence is an implicit assumption in most of the models applied to the analysis of FLM curves, however there had previously been no experimental support of this assumption. A further result is, that the variance of the transit time through any phase of the cycle is proportional to the mean transit time. This implies that the progress of the crypt epithelial cells is subject to an equal degree of randomness in the various phases of the cycle."}
{"id": "PMID:476782", "title": "Interference of sex-related factors in the response of liver cells to experimental mitotic stimuli.", "content": "Stimulation of liver cell multiplication was obtained under two different experimental conditions. (1) A single injection of casein solution resulted in (a) an identical synchronized mitotic wave response in 10-day old male and female rats and (b) a significantly lower response in adult male rats compared to females, a difference which was reduced by castration of males at birth but essentially maintained if animals were operated when 10 days old. (2) Partial hepatectomy shortly after puberty resulted in active hepatocyte multiplication occurring 3 hr earlier in females were ovariectomized at birth and significantly reduced when they were spayed at a later age. Hepatocytes of castrated females entered actively into S phase 2 hr later than the sham-operated controls. Unilateral ovariectomy on the other hand indicated that during compensatory and/or hypercompensatory activity of the single ovary there was a maximum difference between the male and female rate of [3H]thymidine uptake in liver nuclei 20 hr after hepatectomy. A further kinetic study (t = 25, 30,40, 65, 90 hr) indicated no significant sex-related difference in the number of S phases per 10,000 cells. The DNA content of regenerating versus control livers was comparable in both sexes at t = 22 and 90 hr but higher in females at t = 40 and 65 hr. A possible early postnatal interference of certain hormonal mechanisms in the receptivity to mitotic stimuli is postulated and discussed.", "contents": "Interference of sex-related factors in the response of liver cells to experimental mitotic stimuli. Stimulation of liver cell multiplication was obtained under two different experimental conditions. (1) A single injection of casein solution resulted in (a) an identical synchronized mitotic wave response in 10-day old male and female rats and (b) a significantly lower response in adult male rats compared to females, a difference which was reduced by castration of males at birth but essentially maintained if animals were operated when 10 days old. (2) Partial hepatectomy shortly after puberty resulted in active hepatocyte multiplication occurring 3 hr earlier in females were ovariectomized at birth and significantly reduced when they were spayed at a later age. Hepatocytes of castrated females entered actively into S phase 2 hr later than the sham-operated controls. Unilateral ovariectomy on the other hand indicated that during compensatory and/or hypercompensatory activity of the single ovary there was a maximum difference between the male and female rate of [3H]thymidine uptake in liver nuclei 20 hr after hepatectomy. A further kinetic study (t = 25, 30,40, 65, 90 hr) indicated no significant sex-related difference in the number of S phases per 10,000 cells. The DNA content of regenerating versus control livers was comparable in both sexes at t = 22 and 90 hr but higher in females at t = 40 and 65 hr. A possible early postnatal interference of certain hormonal mechanisms in the receptivity to mitotic stimuli is postulated and discussed."}
{"id": "PMID:476783", "title": "Effects of intestinally absorbed thymidine on tritiated thymidine utilization.", "content": "Experimental evidence presented suggests that [3H]TdR can be rapidly and efficiently transported from the intestine to the systemic circulation. This pathway for thymidine transport may be physiologically important since administration of cold thymidine in the drinking water enhances the utilization of a parenterally injected dose of [3H]TdR in several body tissues of the mouse.", "contents": "Effects of intestinally absorbed thymidine on tritiated thymidine utilization. Experimental evidence presented suggests that [3H]TdR can be rapidly and efficiently transported from the intestine to the systemic circulation. This pathway for thymidine transport may be physiologically important since administration of cold thymidine in the drinking water enhances the utilization of a parenterally injected dose of [3H]TdR in several body tissues of the mouse."}
{"id": "PMID:476784", "title": "Alteration of neonatal rat parotid gland acinar cell proliferation by guandethidine-induced sympathectomy.", "content": "Newborn rats were injected with guanethidine-sulfate (20 micrograms/g body weight) every 48 hr from 12 hr after birth until day 14 (eight injections per animal). The guanethidine treatment resulted in an 86% absolute reduction in cell number in the superior cervical ganglia of 15 day old rats. The cells which remained after guanethidine treatment showed destruction of mitochondria and an extensive decrease in endoplasmic reticulum. Chemical sympathectomy with guanethidine induced a 3.1 hr lengthening of the acinar cell generation cycle time (17.4 hr to 20.5 hr), resulting from a longer G1 period (6.9 hr in the control group as compared to 10.5 hr in the guanethidine-treated group), as well as a cecrease in the mean percentage of [3H]thymidine-labeled acinar cells (22.3 +/- 0.5% to 19.3 +/- 0.5%) and mean acinar cell mitotic index (2.6 +/- 0.2% to 2.1 +/- 0.1%). A circadian rhythm was found to exist in parotid gland acinar cell mitotic activity of 15 day old rats and the amplitude of the rhythm was reduced from 26.5% to 14.9% in guanethidine-treated rats. This study indicates that the diminution of sympathetic influence on the developing parotid gland results in a slight, but significant alteration in acnar cell proliferation.", "contents": "Alteration of neonatal rat parotid gland acinar cell proliferation by guandethidine-induced sympathectomy. Newborn rats were injected with guanethidine-sulfate (20 micrograms/g body weight) every 48 hr from 12 hr after birth until day 14 (eight injections per animal). The guanethidine treatment resulted in an 86% absolute reduction in cell number in the superior cervical ganglia of 15 day old rats. The cells which remained after guanethidine treatment showed destruction of mitochondria and an extensive decrease in endoplasmic reticulum. Chemical sympathectomy with guanethidine induced a 3.1 hr lengthening of the acinar cell generation cycle time (17.4 hr to 20.5 hr), resulting from a longer G1 period (6.9 hr in the control group as compared to 10.5 hr in the guanethidine-treated group), as well as a cecrease in the mean percentage of [3H]thymidine-labeled acinar cells (22.3 +/- 0.5% to 19.3 +/- 0.5%) and mean acinar cell mitotic index (2.6 +/- 0.2% to 2.1 +/- 0.1%). A circadian rhythm was found to exist in parotid gland acinar cell mitotic activity of 15 day old rats and the amplitude of the rhythm was reduced from 26.5% to 14.9% in guanethidine-treated rats. This study indicates that the diminution of sympathetic influence on the developing parotid gland results in a slight, but significant alteration in acnar cell proliferation."}
{"id": "PMID:476785", "title": "Low level incorporation of tritiated thymidine into the nuclear DNA of Purkinje neurons of adult mice.", "content": "Adult mice were pulse labeled with tritiated thymidine [3H]TdR and killed 9 hr later. A low level incorporation of [3H]TdT into the nuclear DNA of Purkinje neurons was found in autoradiographs. Enzymatic digestions with DNase and with RNase in combination with autoradiographic grain counts indicate that a portion of nuclear DNA is not stable in the Purkinje nucleus. These results are discussed in light of reports of the stable nature of DNA in Purkinje neurons of adult mice.", "contents": "Low level incorporation of tritiated thymidine into the nuclear DNA of Purkinje neurons of adult mice. Adult mice were pulse labeled with tritiated thymidine [3H]TdR and killed 9 hr later. A low level incorporation of [3H]TdT into the nuclear DNA of Purkinje neurons was found in autoradiographs. Enzymatic digestions with DNase and with RNase in combination with autoradiographic grain counts indicate that a portion of nuclear DNA is not stable in the Purkinje nucleus. These results are discussed in light of reports of the stable nature of DNA in Purkinje neurons of adult mice."}
{"id": "PMID:476786", "title": "Ultrastructure of the swim bladder of the goldfish, Carassius auratus.", "content": "The swim bladder of the cyprinid Carassius auratus (goldfish) is a two-chambered organ connected to the esophagus by a pneumatic duct. The anterior chamber is lined by a single type of squamous epithelial cell. Two types of epithelial cells are present in the posterior chamber. Flattened cells with differences in the electron density of the cytoplasm line most of the chamber. Darker cells generally contain large amounts of glycogen. Cuboidal epithelial cells also occur in the posterior chamber. A glandular layer external to the muscularis in the posterior chamber is composed of large cells containing little glycogen, an extensive Golgi apparatus, and numerous mitochondria with single large granules. Capillaries and nerves are present in large numbers in this layer. Blood vessels form micro-retia mirabilia in the submuscular layer external to the glandular layer. Vessels are of two distinct types with wide lumina and flattened endothelium characterizing the venous vessels. Arterial vessels and have smaller lumina, thick endothelial cells with prominent pinocytotic vesicles, and surrounding pericytes. Collagen is present in three forms in this swim bladder - large tactoids in the tunica externa of the anterior chamber, smaller tactoids in the lamina propria of the posterior chamber, and small fibrils in all other areas.", "contents": "Ultrastructure of the swim bladder of the goldfish, Carassius auratus. The swim bladder of the cyprinid Carassius auratus (goldfish) is a two-chambered organ connected to the esophagus by a pneumatic duct. The anterior chamber is lined by a single type of squamous epithelial cell. Two types of epithelial cells are present in the posterior chamber. Flattened cells with differences in the electron density of the cytoplasm line most of the chamber. Darker cells generally contain large amounts of glycogen. Cuboidal epithelial cells also occur in the posterior chamber. A glandular layer external to the muscularis in the posterior chamber is composed of large cells containing little glycogen, an extensive Golgi apparatus, and numerous mitochondria with single large granules. Capillaries and nerves are present in large numbers in this layer. Blood vessels form micro-retia mirabilia in the submuscular layer external to the glandular layer. Vessels are of two distinct types with wide lumina and flattened endothelium characterizing the venous vessels. Arterial vessels and have smaller lumina, thick endothelial cells with prominent pinocytotic vesicles, and surrounding pericytes. Collagen is present in three forms in this swim bladder - large tactoids in the tunica externa of the anterior chamber, smaller tactoids in the lamina propria of the posterior chamber, and small fibrils in all other areas."}
{"id": "PMID:476787", "title": "The androgenic effect on the fine structure on the Harderian gland in the male hamster.", "content": "A sexual dimorphism of the hamster Harderian gland at the ultrastructural level has been reported. The effect of testosterone on the fine structure of the gland from castrated male golden hamsters is reported here. Harderian glands from the following three groups of animals were examined at regular intervals up to 60 days after castration: (1) castrated; (2) castrated-sham-injected, receiving 0.1 ml sesame oil per day; (3) castrated-testosterone-injected, receiving 2 mg testosterone propionate in 0.1 ml sesame oil per day. In groups 1 and 2, clusters of cylindrical tubules, typical of the male gland, decreased in number and disappeared almost completely 2 wailed in these two groups throughout the remaining period of experiment. On the other hand, these changes were prevented in the group of castrated animals maintained on testosterone propionate. It is concluded that castration modified the ultrastructure of the male hamster Harderian gland toward the female type and that daily administration of testosterone propionate prevented this change.", "contents": "The androgenic effect on the fine structure on the Harderian gland in the male hamster. A sexual dimorphism of the hamster Harderian gland at the ultrastructural level has been reported. The effect of testosterone on the fine structure of the gland from castrated male golden hamsters is reported here. Harderian glands from the following three groups of animals were examined at regular intervals up to 60 days after castration: (1) castrated; (2) castrated-sham-injected, receiving 0.1 ml sesame oil per day; (3) castrated-testosterone-injected, receiving 2 mg testosterone propionate in 0.1 ml sesame oil per day. In groups 1 and 2, clusters of cylindrical tubules, typical of the male gland, decreased in number and disappeared almost completely 2 wailed in these two groups throughout the remaining period of experiment. On the other hand, these changes were prevented in the group of castrated animals maintained on testosterone propionate. It is concluded that castration modified the ultrastructure of the male hamster Harderian gland toward the female type and that daily administration of testosterone propionate prevented this change."}
{"id": "PMID:476788", "title": "Fenestration of the epithelium lining the roof of the fourth cerebral ventricle in amphibia.", "content": "Scanning electron microscopy of the caudal end of the roof of the fourth cerebral ventricle in four amphibian species shows that numerous pores occur between the ependymal cells. These pores have diameters ranging from 5--100 micron; they permit bluk flow of cerebrospinal fluid out of the ventricular system into the subarachnoid space.", "contents": "Fenestration of the epithelium lining the roof of the fourth cerebral ventricle in amphibia. Scanning electron microscopy of the caudal end of the roof of the fourth cerebral ventricle in four amphibian species shows that numerous pores occur between the ependymal cells. These pores have diameters ranging from 5--100 micron; they permit bluk flow of cerebrospinal fluid out of the ventricular system into the subarachnoid space."}
{"id": "PMID:476790", "title": "Development of cell-to-cell relationships in the thyroid gland of the chick embryo.", "content": "Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The focal tight junction develops into the zonula occludens when the primitive follicle lumen first forms. The zonula occludens is, at first, composed of 4.6 +/- 2.45 strands, but with increasing embryonic age the number of strands increases to 5.9 +/- 1.41 in 13 day-old, and 8.0 +/- 1.75 in 19 day-old embryos. Thyroglobulin stored within the embryonic gland lumina is isolated from the mesenchymal tissue even at the first appearance of these follicle cavities. Well developed gap junctions already occur in the thyroid gland of the 7 day-old embryo, so that an intimate relationship and communication between these cells already exists at the time of their functional differentiation.", "contents": "Development of cell-to-cell relationships in the thyroid gland of the chick embryo. Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The focal tight junction develops into the zonula occludens when the primitive follicle lumen first forms. The zonula occludens is, at first, composed of 4.6 +/- 2.45 strands, but with increasing embryonic age the number of strands increases to 5.9 +/- 1.41 in 13 day-old, and 8.0 +/- 1.75 in 19 day-old embryos. Thyroglobulin stored within the embryonic gland lumina is isolated from the mesenchymal tissue even at the first appearance of these follicle cavities. Well developed gap junctions already occur in the thyroid gland of the 7 day-old embryo, so that an intimate relationship and communication between these cells already exists at the time of their functional differentiation."}
{"id": "PMID:476791", "title": "A histochemical study of the innervation of the cerebral blood vessels in the domestic fowl.", "content": "Adrenergic and cholinergic nerves innervating the cerebral arteries of the domestic fowl were examined by specific histochemical techniques. The adrenergic nerve plexuses of the cerebral carotid system are markedly denser than those of other vertebrates observed by similar techniques. They form longitudinally elongated meshworks of fine fibres in the vascular wall of the arterial branches. Those innervating the vertebro-basilar system are less dense and more elongated, and, as the size of the artery diminishes, the fibres of the plexus become coarser. In the small pial and parenchymal arteries they are reduced to a few fibres running parallel to, or spiralling around the vascular axis. The cholinergic nerve plexuses are not as dense as the adrenergic system. The acetylcholinesterase activity is very weak, except in the plexuses innervating the cerebral carotid artery and proximal portion of the anterior and posterior rami. In the vertebro-basilar system, a few thick nerve bundles run alongside the blood vessels of the vertebral and basilar arteries. Cholinergic nerves enter the cranial cavity along the internal carotid, the vertebral and possibly the cerebroethmoidal arteries. Intracerebral capillaries and some arterioles are not innervated with cholinergic and adrenergic fibres of peripheral origin, but with ones arising from parenchymal nerve cells.", "contents": "A histochemical study of the innervation of the cerebral blood vessels in the domestic fowl. Adrenergic and cholinergic nerves innervating the cerebral arteries of the domestic fowl were examined by specific histochemical techniques. The adrenergic nerve plexuses of the cerebral carotid system are markedly denser than those of other vertebrates observed by similar techniques. They form longitudinally elongated meshworks of fine fibres in the vascular wall of the arterial branches. Those innervating the vertebro-basilar system are less dense and more elongated, and, as the size of the artery diminishes, the fibres of the plexus become coarser. In the small pial and parenchymal arteries they are reduced to a few fibres running parallel to, or spiralling around the vascular axis. The cholinergic nerve plexuses are not as dense as the adrenergic system. The acetylcholinesterase activity is very weak, except in the plexuses innervating the cerebral carotid artery and proximal portion of the anterior and posterior rami. In the vertebro-basilar system, a few thick nerve bundles run alongside the blood vessels of the vertebral and basilar arteries. Cholinergic nerves enter the cranial cavity along the internal carotid, the vertebral and possibly the cerebroethmoidal arteries. Intracerebral capillaries and some arterioles are not innervated with cholinergic and adrenergic fibres of peripheral origin, but with ones arising from parenchymal nerve cells."}
{"id": "PMID:476792", "title": "Impermeability of hagfish cerebral capillaries to horseradish peroxidase. An ultrastructural study.", "content": "Brain capillaires and their permeability to intravenously injected horseradish peroxidase, HRP, (MW: 40,000) were examined electron-microscopically in an attempt to find a structural explanation for the poorly developed blood-brain barrier in the hagfish, Myxine glutinosa. In particular, it was the aim of this study to examine the role of the numerous endothelial vesicles and tubules in the transport of this tracer between blood and brain. Many of the vesicles and tubules were found to be in continuity with the luminal or abluminal surfaces, but tubules generating channels through the endothelial cells were never observed. The cleft between adjacent endothelial cells was obliterated by punctate junctions. HRP, which was aloowed to circulate for up to 35 min, was not found in the basal lamina or in the surrounding brain parenchyma. Few of the luminal vesicles and tubules were marked by the tracer. In the intercellular cleft HRP was stopped by the junctions. It is concluded that the hagfish like other vertebrates has a blood-brain barrier to HRP, and the numerous vesicles and tubules occurring in hagfish brain endothelium are not involved in the transendothelial transport of this macromolecule.", "contents": "Impermeability of hagfish cerebral capillaries to horseradish peroxidase. An ultrastructural study. Brain capillaires and their permeability to intravenously injected horseradish peroxidase, HRP, (MW: 40,000) were examined electron-microscopically in an attempt to find a structural explanation for the poorly developed blood-brain barrier in the hagfish, Myxine glutinosa. In particular, it was the aim of this study to examine the role of the numerous endothelial vesicles and tubules in the transport of this tracer between blood and brain. Many of the vesicles and tubules were found to be in continuity with the luminal or abluminal surfaces, but tubules generating channels through the endothelial cells were never observed. The cleft between adjacent endothelial cells was obliterated by punctate junctions. HRP, which was aloowed to circulate for up to 35 min, was not found in the basal lamina or in the surrounding brain parenchyma. Few of the luminal vesicles and tubules were marked by the tracer. In the intercellular cleft HRP was stopped by the junctions. It is concluded that the hagfish like other vertebrates has a blood-brain barrier to HRP, and the numerous vesicles and tubules occurring in hagfish brain endothelium are not involved in the transendothelial transport of this macromolecule."}
{"id": "PMID:476793", "title": "Premetamorphic changes in the ultrastructure of Calliphora fat cells.", "content": "In the fat body of Calliphora a drastic reorganization of the cell structure, which is related to premetamorphic functional changes, can be observed during the 3rd larval stage of development. These changes include an accumulation of protein storage granules, a decrease in the relative volume of the mitochondria and the surface area of endoplasmic reticulum membrane, and the disappearance of vacuoles. \"Absolute\" values obtained for organelle areas and membrane perimeters confirm the assumption that large numbers of organelles are eliminated by autophagic activity prior to the pupation period. A temporary restoration of the endoplasmic reticulum is found immediately before pupariation. Large variations in organelle density were found between individual fat cells and between the nuclear, mid- and peripheral regions of the cytoplasm.", "contents": "Premetamorphic changes in the ultrastructure of Calliphora fat cells. In the fat body of Calliphora a drastic reorganization of the cell structure, which is related to premetamorphic functional changes, can be observed during the 3rd larval stage of development. These changes include an accumulation of protein storage granules, a decrease in the relative volume of the mitochondria and the surface area of endoplasmic reticulum membrane, and the disappearance of vacuoles. \"Absolute\" values obtained for organelle areas and membrane perimeters confirm the assumption that large numbers of organelles are eliminated by autophagic activity prior to the pupation period. A temporary restoration of the endoplasmic reticulum is found immediately before pupariation. Large variations in organelle density were found between individual fat cells and between the nuclear, mid- and peripheral regions of the cytoplasm."}
{"id": "PMID:476795", "title": "The pigmentary system of planaria. I. Morphology.", "content": "The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), has been studied by light and electron microscopy. The system consists of granules contained in chromatophore-like cells embedded in the parenchyma. The cell processes penetrate between the muscle layers and extend to the sub-epidermal basal lamina. The nature of the pigment and the comparative anatomical significance of the chromatophore structure is discussed.", "contents": "The pigmentary system of planaria. I. Morphology. The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), has been studied by light and electron microscopy. The system consists of granules contained in chromatophore-like cells embedded in the parenchyma. The cell processes penetrate between the muscle layers and extend to the sub-epidermal basal lamina. The nature of the pigment and the comparative anatomical significance of the chromatophore structure is discussed."}
{"id": "PMID:476789", "title": "Experimental studies of apocrine secretion in the dorsal prostate epithelium of the rat.", "content": "Rat dorsal prostate epithelium was studied in intact adult animals, in animals castrated for three days and in rats after inhibition of prolactin secretion. Thin sections, electron-microscopic autoradiographs and freeze-fracture replicas were used to analyze the process of apocrine secretion in this gland. The rough endoplasmic reticulum and the Golgi apparatus of the secretory cells are well developed, but secretory granules are absent. The only sign indicating release of secretory material is the appearance of blebs originating from the apical plasma membrane. Freeze-fracture replicas of the apical plasma membrane reveal that the blebs develop randomly from the bases of microvilli-like protrusions. In vitro pulse labeling of the proteins using 3H-leucine resulted in a labeling of the apical blebs. A post-castration period of three days was sufficient to reduce drastically the number and size of the apical blebs conhree weeks by application of lisuride, a synthetic ergot alkaloid, also induced regressive changes in the secretory cells. The apical blebs were still present, but they were shrunken and their content appeared condensed. These experimental conditions proved that the apical blebs are closely related to the functional activity of the cells and are interpreted as true apocrine secretion in the rat dorsal epithelium.", "contents": "Experimental studies of apocrine secretion in the dorsal prostate epithelium of the rat. Rat dorsal prostate epithelium was studied in intact adult animals, in animals castrated for three days and in rats after inhibition of prolactin secretion. Thin sections, electron-microscopic autoradiographs and freeze-fracture replicas were used to analyze the process of apocrine secretion in this gland. The rough endoplasmic reticulum and the Golgi apparatus of the secretory cells are well developed, but secretory granules are absent. The only sign indicating release of secretory material is the appearance of blebs originating from the apical plasma membrane. Freeze-fracture replicas of the apical plasma membrane reveal that the blebs develop randomly from the bases of microvilli-like protrusions. In vitro pulse labeling of the proteins using 3H-leucine resulted in a labeling of the apical blebs. A post-castration period of three days was sufficient to reduce drastically the number and size of the apical blebs conhree weeks by application of lisuride, a synthetic ergot alkaloid, also induced regressive changes in the secretory cells. The apical blebs were still present, but they were shrunken and their content appeared condensed. These experimental conditions proved that the apical blebs are closely related to the functional activity of the cells and are interpreted as true apocrine secretion in the rat dorsal epithelium."}
{"id": "PMID:476794", "title": "Autoradiographic detection of indolamine and catecholamine neurons in the nervous system of Owenia fusiformis (Polychaeta, Annelida).", "content": "Light- and electron-microscopic autoradiographic studies were carried out on Owenia to detect selectively catecholaminergic and indolaminergic neurons at two appropriate body levels according to the regenerative properties of this annelid, i.e. in the 1st and the 4th abdominal segments. Autoradiographically, intense 3H-5-hydroxytryptamine accumulation is seen in the 1st abdominal segment, and a less intense autoradiographic reaction in the 4th segment. Roughly, a similar difference in the distribution of the label is seen at both levels following 3H-noradrenaline administration. These observations, confirmed by a quantitative uptake analysis, suggest the presence of catecholaminergic and indolaminergic neurons in Owenia.", "contents": "Autoradiographic detection of indolamine and catecholamine neurons in the nervous system of Owenia fusiformis (Polychaeta, Annelida). Light- and electron-microscopic autoradiographic studies were carried out on Owenia to detect selectively catecholaminergic and indolaminergic neurons at two appropriate body levels according to the regenerative properties of this annelid, i.e. in the 1st and the 4th abdominal segments. Autoradiographically, intense 3H-5-hydroxytryptamine accumulation is seen in the 1st abdominal segment, and a less intense autoradiographic reaction in the 4th segment. Roughly, a similar difference in the distribution of the label is seen at both levels following 3H-noradrenaline administration. These observations, confirmed by a quantitative uptake analysis, suggest the presence of catecholaminergic and indolaminergic neurons in Owenia."}
{"id": "PMID:476796", "title": "Probit analysis of the atrioventricular (AV) junctional tissues of the ferret heart.", "content": "Probit frequency analysis, a graphic method for determining whether a population is normally distributed, skewed, or multinodal, was used to determine whether P cells are present in different regions of the AV junction in the ferret heart. This analysis indicated that at least 95% of the cells of the transitional zone, superficial AV node, deep AV node, and distal AV bundle of the ferret heart are morphologically homogeneous. In the proximal AV bundle a large cell population is found in addition to the AV bundle cells. The probit analysis was also used to characterize the shape of the cells of each region of the AV junction further. AV nodal cells are not as elongated as the atrial muscle cells and AV bundle cells. These nodal cells also do not branch as extensively as the AV bundle cells.", "contents": "Probit analysis of the atrioventricular (AV) junctional tissues of the ferret heart. Probit frequency analysis, a graphic method for determining whether a population is normally distributed, skewed, or multinodal, was used to determine whether P cells are present in different regions of the AV junction in the ferret heart. This analysis indicated that at least 95% of the cells of the transitional zone, superficial AV node, deep AV node, and distal AV bundle of the ferret heart are morphologically homogeneous. In the proximal AV bundle a large cell population is found in addition to the AV bundle cells. The probit analysis was also used to characterize the shape of the cells of each region of the AV junction further. AV nodal cells are not as elongated as the atrial muscle cells and AV bundle cells. These nodal cells also do not branch as extensively as the AV bundle cells."}
{"id": "PMID:476797", "title": "The fine structure of the parietal retinas of Anolis carolinensis and Iguana iguana.", "content": "An electron microscopic examination of the parietal retinas of Anolis carolinensis and Iguana iguana demonstrated within each retina (1) two distinct populations of neurons, (2) two populations of glia, and (3) a population of photoreceptors which could not be subdivided. A small population of very electron-dense cells, in many respects similar to photoreceptors, was also found in the iguana. Correspondingly dark processes were found in the plexiform layer of each retina. Parietal photoreceptors generally resemble cones of the lateral eye. Glial cells were sub-classified on the basis of the location of their somata and the disposition of their processes. Neurons were identified by virtue of their cytology and their reception of axosomatic ribbon synapses from unidentified plexiform layer processes. Neuronal subtypes were located on opposite sides of the plexiform layer. Neurons distal to that layer were found to project the initial segments of their processes into the plexiform layer parallel to its long axis, while neurons central to the plexiform layer projected axons centrally and dendrites radially into the plexiform layer. The existence of at least two neuronal populations and of interphotoreceptor synapses suggests that photosensory processing within the parietal retina may be more complex than previously assumed.", "contents": "The fine structure of the parietal retinas of Anolis carolinensis and Iguana iguana. An electron microscopic examination of the parietal retinas of Anolis carolinensis and Iguana iguana demonstrated within each retina (1) two distinct populations of neurons, (2) two populations of glia, and (3) a population of photoreceptors which could not be subdivided. A small population of very electron-dense cells, in many respects similar to photoreceptors, was also found in the iguana. Correspondingly dark processes were found in the plexiform layer of each retina. Parietal photoreceptors generally resemble cones of the lateral eye. Glial cells were sub-classified on the basis of the location of their somata and the disposition of their processes. Neurons were identified by virtue of their cytology and their reception of axosomatic ribbon synapses from unidentified plexiform layer processes. Neuronal subtypes were located on opposite sides of the plexiform layer. Neurons distal to that layer were found to project the initial segments of their processes into the plexiform layer parallel to its long axis, while neurons central to the plexiform layer projected axons centrally and dendrites radially into the plexiform layer. The existence of at least two neuronal populations and of interphotoreceptor synapses suggests that photosensory processing within the parietal retina may be more complex than previously assumed."}
{"id": "PMID:476798", "title": "Estrogen-induced ultrastructural changes in cultured chick embryo hepatocytes.", "content": "Administration of estradiol-17-beta induces the synthesis of vitellogenin in primary cultures of chick embryo liver. The ultrastructural changes accompanying steroid induced vitellogenin synthesis were investigated in hepatocytes cultures incubated for 0, 12 and 24 h following hormonal treatment. Both electron microscopy and immuno-chemical techniques were used. The immuno-fluorescence data indicate that the cultures contain a cell population of 90--95% hepatocytes, both in control and estradiol-treated groups. Ultrastructurally, cultured hepatocytes are similar to in vivo ones, except for a reduced accumulation of storage materials, glycogen and lipids. In the estradiol-treated cultures hepatocytes show an accumulation of free ribosomes, enlargement of rough endoplasmic reticulum, and development of the Golgi apparatus.", "contents": "Estrogen-induced ultrastructural changes in cultured chick embryo hepatocytes. Administration of estradiol-17-beta induces the synthesis of vitellogenin in primary cultures of chick embryo liver. The ultrastructural changes accompanying steroid induced vitellogenin synthesis were investigated in hepatocytes cultures incubated for 0, 12 and 24 h following hormonal treatment. Both electron microscopy and immuno-chemical techniques were used. The immuno-fluorescence data indicate that the cultures contain a cell population of 90--95% hepatocytes, both in control and estradiol-treated groups. Ultrastructurally, cultured hepatocytes are similar to in vivo ones, except for a reduced accumulation of storage materials, glycogen and lipids. In the estradiol-treated cultures hepatocytes show an accumulation of free ribosomes, enlargement of rough endoplasmic reticulum, and development of the Golgi apparatus."}
{"id": "PMID:476799", "title": "Reformation of gap and tight junctions in regenerating liver after cholestasis.", "content": "Morphometric analysis of the alterations in interhepatocyte junctions induced by bile duct ligation revealed that after 48 h, during which time the serum bilirubin increased 6 to 8 fold, the membrane area occupied by gap junctions on the apico-lateral and medio-lateral sides decreased from 3.6% in controls to 0.02% in the ligated group. The strands of the zonulae occludentes were reduced in number and showed increased discontinuities. Within 45 min of recanalization of the common bile duct, clusters of particles appeared within and adjacent to the tight junctional areas or in the lateral hepatocyte membrane. Subsequently, the particle aggregations localized in the apico-lateral membrane areas increased in number and size becoming finally indistinguishable from those of controls within 96 h after the onset of recanalization. The zonulae occludentes also rearranged and reestablished their original structure during this period. The serum bilirubin fell to normal within 24 h of recanalization. It is concluded that metabolic and ultrastructural restitution associated with the recanalization of the ligated bile duct have no strict temporal correlation to one another. These studies provide further evidence that alterations in gap and tight junctions induced by pathological processes, e.g. during bile duct ligation, are completely reversible when regeneration occurs.", "contents": "Reformation of gap and tight junctions in regenerating liver after cholestasis. Morphometric analysis of the alterations in interhepatocyte junctions induced by bile duct ligation revealed that after 48 h, during which time the serum bilirubin increased 6 to 8 fold, the membrane area occupied by gap junctions on the apico-lateral and medio-lateral sides decreased from 3.6% in controls to 0.02% in the ligated group. The strands of the zonulae occludentes were reduced in number and showed increased discontinuities. Within 45 min of recanalization of the common bile duct, clusters of particles appeared within and adjacent to the tight junctional areas or in the lateral hepatocyte membrane. Subsequently, the particle aggregations localized in the apico-lateral membrane areas increased in number and size becoming finally indistinguishable from those of controls within 96 h after the onset of recanalization. The zonulae occludentes also rearranged and reestablished their original structure during this period. The serum bilirubin fell to normal within 24 h of recanalization. It is concluded that metabolic and ultrastructural restitution associated with the recanalization of the ligated bile duct have no strict temporal correlation to one another. These studies provide further evidence that alterations in gap and tight junctions induced by pathological processes, e.g. during bile duct ligation, are completely reversible when regeneration occurs."}
{"id": "PMID:476800", "title": "Subsurface cisterns in paraventricular nuclei of the hypothalamus of the rat.", "content": "Structures identified as subsurface cisterns (SSC's) were found in neurons of the paraventricular nuclei of the rat hypothalamus. They appeared as cytoplasmic organelles consisting most often of stacks of parallel cisterns apposed to the neuronal plasmalemma. These SSC's were located in the interneurons of the parvocellular system, but not in neurosecretory cells and glial cells. SSC's were seen at zones of cytoplasm apposed to neuronal or glial cell processes, showing in some instances specific relationships with synaptic areas. The morphological features of these SSC's are described, and their possible functional significance is briefly discussed.", "contents": "Subsurface cisterns in paraventricular nuclei of the hypothalamus of the rat. Structures identified as subsurface cisterns (SSC's) were found in neurons of the paraventricular nuclei of the rat hypothalamus. They appeared as cytoplasmic organelles consisting most often of stacks of parallel cisterns apposed to the neuronal plasmalemma. These SSC's were located in the interneurons of the parvocellular system, but not in neurosecretory cells and glial cells. SSC's were seen at zones of cytoplasm apposed to neuronal or glial cell processes, showing in some instances specific relationships with synaptic areas. The morphological features of these SSC's are described, and their possible functional significance is briefly discussed."}
{"id": "PMID:476801", "title": "The fine structure of the hypostome and mouth of hydra. I. Scanning electron microscopy.", "content": "The hypostome and mouth of fresh-water Hydra were examined by scanning electron microscopy. The external surface of the hypostome possesses cnidocils, possibly sensory hairs, and small spiny protrusions surrounding the mouth; the internal surface has cylindrical microvilli, free flagella and adherent flagella. The adherent flagella are most numerous close to the mouth where they cause the cell surface to appear smooth when viewed at low magnifications. Free flagella and leaf-like microvilli increase in prominence towards the tentacles and enter on proper. The edge of the mouth has an abrupt boundary marking the apposition of epidermal and gastrodermal cells. A transitional groove occurs at the boundary and the cells underlying the groove are smaller than those on other regions of the hypostome. The transition groove may represent a site of cell loss in normal cell turnover. Some of the small underlying cells may represent nervous elements involved in regulating hypostome activity during the feeding reation.", "contents": "The fine structure of the hypostome and mouth of hydra. I. Scanning electron microscopy. The hypostome and mouth of fresh-water Hydra were examined by scanning electron microscopy. The external surface of the hypostome possesses cnidocils, possibly sensory hairs, and small spiny protrusions surrounding the mouth; the internal surface has cylindrical microvilli, free flagella and adherent flagella. The adherent flagella are most numerous close to the mouth where they cause the cell surface to appear smooth when viewed at low magnifications. Free flagella and leaf-like microvilli increase in prominence towards the tentacles and enter on proper. The edge of the mouth has an abrupt boundary marking the apposition of epidermal and gastrodermal cells. A transitional groove occurs at the boundary and the cells underlying the groove are smaller than those on other regions of the hypostome. The transition groove may represent a site of cell loss in normal cell turnover. Some of the small underlying cells may represent nervous elements involved in regulating hypostome activity during the feeding reation."}
{"id": "PMID:476802", "title": "The fine structure of the hypostome and mouth of hydra. II. Transmission electron microscopy.", "content": "The normal morphology of the hypostome and mouth of hydra were examined by transmission electron microscopy with conventional thin sections and freeze-fracture replicas. Myonemes of the hypostome are small in diameter, have gap and intermediate-type cell junctions within each epithelial layer and are associated with the opposite epithelial layer by transmesogleal processes and gap junctions. Nematocysts and sensory cells are aggregated in the circumoral region. The fine structure of adherent flagella arising from gastrodermal gland cells, and the transition region at the mouth between epidermis and gastrodermis are described in detail for the first time. The possible functional significance of the findings is discussed.", "contents": "The fine structure of the hypostome and mouth of hydra. II. Transmission electron microscopy. The normal morphology of the hypostome and mouth of hydra were examined by transmission electron microscopy with conventional thin sections and freeze-fracture replicas. Myonemes of the hypostome are small in diameter, have gap and intermediate-type cell junctions within each epithelial layer and are associated with the opposite epithelial layer by transmesogleal processes and gap junctions. Nematocysts and sensory cells are aggregated in the circumoral region. The fine structure of adherent flagella arising from gastrodermal gland cells, and the transition region at the mouth between epidermis and gastrodermis are described in detail for the first time. The possible functional significance of the findings is discussed."}
{"id": "PMID:476803", "title": "Morphometric estimation of stromal edema during delayed implantation in the rat.", "content": "Morphometric methods were used at the light microscopic level to investigate the appearance of edema in the endometrial stroma of rats during estradiol-induced implantation after an experimental delay. Comparisons between blastocyst-free and blastocyst-containing sites were made at 18, 12, 16 and 24 h after injection of estradiol (h.a.e.). The development of stromal edema during implantation was found to be diphasic. First, during the initial 8--12 h.a.e., a generalized edema developed all along the uterine horns. Later, from 16 h.a.e. onwards, a local edema was present around the blastocysts. The Pontamine Blue Reaction (PBR) became visible between 20 and 24 h.a.e. The results indicate that the blastocyst is recognized by the stroma considerably before the PBR. The appearance of a local edema around the blastocysts before the PBR might be related to a slow increase in vascular permeability and/or to the increased stromal cell synthetic activity that is known to precede the PBR during early implantation.", "contents": "Morphometric estimation of stromal edema during delayed implantation in the rat. Morphometric methods were used at the light microscopic level to investigate the appearance of edema in the endometrial stroma of rats during estradiol-induced implantation after an experimental delay. Comparisons between blastocyst-free and blastocyst-containing sites were made at 18, 12, 16 and 24 h after injection of estradiol (h.a.e.). The development of stromal edema during implantation was found to be diphasic. First, during the initial 8--12 h.a.e., a generalized edema developed all along the uterine horns. Later, from 16 h.a.e. onwards, a local edema was present around the blastocysts. The Pontamine Blue Reaction (PBR) became visible between 20 and 24 h.a.e. The results indicate that the blastocyst is recognized by the stroma considerably before the PBR. The appearance of a local edema around the blastocysts before the PBR might be related to a slow increase in vascular permeability and/or to the increased stromal cell synthetic activity that is known to precede the PBR during early implantation."}
{"id": "PMID:476804", "title": "On the development of midpiece mitochondria of mouse spermatozoa.", "content": "As seen by scanning electron microscopy, the mitochondrial helix in the developing midpiece of mouse testicular spermatozoa is dextral in direction and consists of spherical mitochondrial units arranged in an orderly array of four units per gyre: three appearing in face view and a fourth hidden from view at the back of the gyre. As the spermatozoa mature, the dextral helix is transformed into a sinistral helix. Its constituent spherical mitochondria either change direction abruptly without changing shape; or having first become semilunar or diamond-shaped, they change direction gradually. Mitochondrial division follows the change in helical pitch producing a double sinistral helix. The spherical (or semilunar/diamond-shaped) mitochondria presumably elongate to form the units of the mature midpiece.", "contents": "On the development of midpiece mitochondria of mouse spermatozoa. As seen by scanning electron microscopy, the mitochondrial helix in the developing midpiece of mouse testicular spermatozoa is dextral in direction and consists of spherical mitochondrial units arranged in an orderly array of four units per gyre: three appearing in face view and a fourth hidden from view at the back of the gyre. As the spermatozoa mature, the dextral helix is transformed into a sinistral helix. Its constituent spherical mitochondria either change direction abruptly without changing shape; or having first become semilunar or diamond-shaped, they change direction gradually. Mitochondrial division follows the change in helical pitch producing a double sinistral helix. The spherical (or semilunar/diamond-shaped) mitochondria presumably elongate to form the units of the mature midpiece."}
{"id": "PMID:476805", "title": "Vacuolated neurons in the hypogastric ganglion of the rat.", "content": "The vacuolated neurons (VN) of the main hypogastric ganglion of the male rat were studied using the formaldehyde-induced fluorescence (FIF) method for the histochemical demonstration of catecholamines. Microspectrofluorimetry was performed to identify the fluorophores and to quantify the FIF. The thiocholine method (Koelle-Gomori) was used to demonstrate acetylcholinesterase activity. The fine structure of the VN was studied using glutaraldehyde/OsO4 fixation. (1) In the untreated adult male rat VN represent only a small population of the total number of hypogastric neurons (0.8--1.2%). The vacuoles are similar to those of the VN from the corresponding female ganglion. (2) The VN are considered to be adrenergic due to the nature of their fluorophore, indicating a primary catecholamine. (3) The first VN appear in the hypogastric ganglia at the age of 7 weeks. After testosterone administration to young rats, VN are found at the age of 4 weeks. (4) The basic fine structure of the VN is similar to that of other ordinary neurons of the hypogastric ganglia. (5) The content of the vacuoles could not be identified. (6) Indications of degeneration were not observed in the VN. (7) The VN are interpreted as being a functional stage of the \"short\" adrenergic neurons, which are under the control of steroid hormones. (8) Fifteen months after castration, no VN could be found in the hypogastric ganglia, while their number was normal in the corresponding control animals.", "contents": "Vacuolated neurons in the hypogastric ganglion of the rat. The vacuolated neurons (VN) of the main hypogastric ganglion of the male rat were studied using the formaldehyde-induced fluorescence (FIF) method for the histochemical demonstration of catecholamines. Microspectrofluorimetry was performed to identify the fluorophores and to quantify the FIF. The thiocholine method (Koelle-Gomori) was used to demonstrate acetylcholinesterase activity. The fine structure of the VN was studied using glutaraldehyde/OsO4 fixation. (1) In the untreated adult male rat VN represent only a small population of the total number of hypogastric neurons (0.8--1.2%). The vacuoles are similar to those of the VN from the corresponding female ganglion. (2) The VN are considered to be adrenergic due to the nature of their fluorophore, indicating a primary catecholamine. (3) The first VN appear in the hypogastric ganglia at the age of 7 weeks. After testosterone administration to young rats, VN are found at the age of 4 weeks. (4) The basic fine structure of the VN is similar to that of other ordinary neurons of the hypogastric ganglia. (5) The content of the vacuoles could not be identified. (6) Indications of degeneration were not observed in the VN. (7) The VN are interpreted as being a functional stage of the \"short\" adrenergic neurons, which are under the control of steroid hormones. (8) Fifteen months after castration, no VN could be found in the hypogastric ganglia, while their number was normal in the corresponding control animals."}
{"id": "PMID:476806", "title": "Chloride cells and chloride exchange in the skin of a sea-water teleost, the shanny (Blennius pholis L.).", "content": "The fine structure of the skin and its importance in chloride outfluxes were investigated in a sea-water teleost, the shanny (Blennius pholis L.). The epidermis is composed of three cells types: epithelial cells, mucous cells and chloride cells. These chloride cells typically contain a great number of mitochondria and an extensive agranular reticulum extending through the whole cell body. They open at the surface of the epidermis into an apical pit. An undifferentiated small cell is often observed near these chloride cells and probably corresponds to the \"adjacent chloride cell\". The values of chloride outfluxes through the skin and the gills are respectively 5333+/-884 muEq.h-1.kh-1 and 4479+/-2521 muEq.h-1.kg-1; n = 6; t degrees = 13+/-0.5 degrees C. Thus the ratio between skin chloride outflux and total chloride outflux is 64.7 +/- 9.3%.", "contents": "Chloride cells and chloride exchange in the skin of a sea-water teleost, the shanny (Blennius pholis L.). The fine structure of the skin and its importance in chloride outfluxes were investigated in a sea-water teleost, the shanny (Blennius pholis L.). The epidermis is composed of three cells types: epithelial cells, mucous cells and chloride cells. These chloride cells typically contain a great number of mitochondria and an extensive agranular reticulum extending through the whole cell body. They open at the surface of the epidermis into an apical pit. An undifferentiated small cell is often observed near these chloride cells and probably corresponds to the \"adjacent chloride cell\". The values of chloride outfluxes through the skin and the gills are respectively 5333+/-884 muEq.h-1.kh-1 and 4479+/-2521 muEq.h-1.kg-1; n = 6; t degrees = 13+/-0.5 degrees C. Thus the ratio between skin chloride outflux and total chloride outflux is 64.7 +/- 9.3%."}
{"id": "PMID:476807", "title": "Freeze-etch study of the teleostean pituitary.", "content": "The structure of membranes in the pituitary of several teleostean species was studied with the freeze-etching technique. Nuclear pore density is higher in hormone-secreting than in stellate cells, suggesting a higher rate of nucleo-cytoplasmic interactions in the secretory cells. The perinuclear endoplasmic reticulum (ER) in the MAS-secreting cells has a large number of fenestrae, whereas in the ER whorls of the prolactin-secreting cells the fenestration occurs only rarely. Three different types of membrane specializations are described, presumably related to exo-endocytotic events at the level of the plasmalemma.", "contents": "Freeze-etch study of the teleostean pituitary. The structure of membranes in the pituitary of several teleostean species was studied with the freeze-etching technique. Nuclear pore density is higher in hormone-secreting than in stellate cells, suggesting a higher rate of nucleo-cytoplasmic interactions in the secretory cells. The perinuclear endoplasmic reticulum (ER) in the MAS-secreting cells has a large number of fenestrae, whereas in the ER whorls of the prolactin-secreting cells the fenestration occurs only rarely. Three different types of membrane specializations are described, presumably related to exo-endocytotic events at the level of the plasmalemma."}
{"id": "PMID:476808", "title": "The smooth endoplasmic reticulum in neurohypophysial axons of the rat: possible involvement in transport, storage and release of neurosecretory material.", "content": "The intra-axonal organization of the smooth endoplasmic reticulum was studied in the neurohypophysis of rats during and after water deprivation. Parallel to conventional electron microscopy, the material was treated with a double impregnation staining technique specifically designed to contrast the intracellular membranous system. In conventionally stained ultrathin sections from severely dehydrated rats most axons appeared to be free of membranous organelles, whereas corresponding axons treated with the double-impregnation technique generally exhibited a highly developed system of smooth endoplasmic reticulum. In axonal endings, both techniques revealed a profusion of microvesicles in intimate relationship with tubular elements of the smooth endoplasmic reticulum. In short-term (12h) rehydrated rats, a similarly developed system of smooth endoplasmic reticulum was stilll observed at all axonal levels with both procedures. After 24 to 48 h or rehydration the tubules of the smooth endoplasmic reticulum exhibited, in double impregnated material, numerous dilatations which resembled the adjacent neurosecretory granules. In conventionally stained ultrathin sections, an accumulation of electron dense material occurred within tubules of the smooth endoplasmic reticulum in the more proximal axonal segments, while in the more terminal segments, which contained numerous elongated granules, membrane continuity was frequently observed between newly formed granules and the smooth endoplasmic reticulum. After 7 days of rehydration the general pattern of the axonal smooth endoplasmic reticulum was comparable to that in untreated rats. These results are discussed in the light of a suggested involvement of the axonal smooth endoplasmic reticulum in the non-granlular transport of neurosecretory material in connection with (1) storage in distally formed granules, and (2) release via microvesicles.", "contents": "The smooth endoplasmic reticulum in neurohypophysial axons of the rat: possible involvement in transport, storage and release of neurosecretory material. The intra-axonal organization of the smooth endoplasmic reticulum was studied in the neurohypophysis of rats during and after water deprivation. Parallel to conventional electron microscopy, the material was treated with a double impregnation staining technique specifically designed to contrast the intracellular membranous system. In conventionally stained ultrathin sections from severely dehydrated rats most axons appeared to be free of membranous organelles, whereas corresponding axons treated with the double-impregnation technique generally exhibited a highly developed system of smooth endoplasmic reticulum. In axonal endings, both techniques revealed a profusion of microvesicles in intimate relationship with tubular elements of the smooth endoplasmic reticulum. In short-term (12h) rehydrated rats, a similarly developed system of smooth endoplasmic reticulum was stilll observed at all axonal levels with both procedures. After 24 to 48 h or rehydration the tubules of the smooth endoplasmic reticulum exhibited, in double impregnated material, numerous dilatations which resembled the adjacent neurosecretory granules. In conventionally stained ultrathin sections, an accumulation of electron dense material occurred within tubules of the smooth endoplasmic reticulum in the more proximal axonal segments, while in the more terminal segments, which contained numerous elongated granules, membrane continuity was frequently observed between newly formed granules and the smooth endoplasmic reticulum. After 7 days of rehydration the general pattern of the axonal smooth endoplasmic reticulum was comparable to that in untreated rats. These results are discussed in the light of a suggested involvement of the axonal smooth endoplasmic reticulum in the non-granlular transport of neurosecretory material in connection with (1) storage in distally formed granules, and (2) release via microvesicles."}
{"id": "PMID:476810", "title": "Photoreceptor membrane breakdown in the spider Dinopis: localisation of acid phosphatases.", "content": "The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and Kao (1978). Weak and infrequent reactions are obtained in multivesicular bodies (mvbs) and multilamellar bodies (mlbs) derived from them. Residual bodies (rbs) begin to react strongly as they lyse. Source of AcPhs is endoplasmic reticulum which has barely differentiated towards the GERL configuration; it becomes reactive as it is incorporated into secondary lysosomes. GERL tubules, Y-bodies and vesicles respond erratically and weakly, and are also incorporated into rbs. No evidence was found for a significant participation of Golgi bodies in these processes, and acid phosphatase cytochemistry fails to reveal a topographical relationship between GERL in these cells and Golgi saccules. Coated vesicle clusters found in the predawn receptive segments are AcPh-negative; this implies that their previous identification as GERL-derived \"Nebenkerne\" carrying hydrolytic enzymes to newly-formed mvbs (Blest, Kao and Powell, 1978) is dubious. Isolation bodies and autophagic vacuoles enclosing other organelles in pathological receptors give strong reactions while adjacent secondary lysosomes derived from rhabdomere membrane and associated GERL give weak ones. It is concluded that rhabdomere-derived rb lysis is more tightly regulated than other autophagic processes, and it is suggested that a high degree of control is necessary in a receptor which may repeat the autophagy of a large mass of transductive membrane at least 60--100 times in the course of its working life.", "contents": "Photoreceptor membrane breakdown in the spider Dinopis: localisation of acid phosphatases. The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and Kao (1978). Weak and infrequent reactions are obtained in multivesicular bodies (mvbs) and multilamellar bodies (mlbs) derived from them. Residual bodies (rbs) begin to react strongly as they lyse. Source of AcPhs is endoplasmic reticulum which has barely differentiated towards the GERL configuration; it becomes reactive as it is incorporated into secondary lysosomes. GERL tubules, Y-bodies and vesicles respond erratically and weakly, and are also incorporated into rbs. No evidence was found for a significant participation of Golgi bodies in these processes, and acid phosphatase cytochemistry fails to reveal a topographical relationship between GERL in these cells and Golgi saccules. Coated vesicle clusters found in the predawn receptive segments are AcPh-negative; this implies that their previous identification as GERL-derived \"Nebenkerne\" carrying hydrolytic enzymes to newly-formed mvbs (Blest, Kao and Powell, 1978) is dubious. Isolation bodies and autophagic vacuoles enclosing other organelles in pathological receptors give strong reactions while adjacent secondary lysosomes derived from rhabdomere membrane and associated GERL give weak ones. It is concluded that rhabdomere-derived rb lysis is more tightly regulated than other autophagic processes, and it is suggested that a high degree of control is necessary in a receptor which may repeat the autophagy of a large mass of transductive membrane at least 60--100 times in the course of its working life."}
{"id": "PMID:476811", "title": "Synaptic ribbons during postnatal development of the pineal gland in the golden hamster (Mesocricetus auratus).", "content": "Synaptic ribbons, functionally enigmatic structures of mammalian pinealocytes, were studied during the postnatal development of the pineal gland in the golden hamster (Mesocricetus auratus). On day 4 post partum, synaptic ribbons appear in cells that have already started to differentiate into pinealocytes. Between days 4 and 9, an increase in the number of synaptic ribbons occurs, concomitant with the continuing differentiation of the pineal tissue. Between days 9 and 16, when differentiation of this tissue is almost completed, the number of synaptic ribbons decreases and approaches that characteristic of the adult pineal gland. During development, the synaptic ribbons increase in length, and dense core vesicles are frequently found in the vicinity of these structures. It is assumed that a functional relationship exists between dense core vesicles and the synaptic ribbons, which are considered to be engaged in a certain form of secretory activity of the mammalian pineal gland.", "contents": "Synaptic ribbons during postnatal development of the pineal gland in the golden hamster (Mesocricetus auratus). Synaptic ribbons, functionally enigmatic structures of mammalian pinealocytes, were studied during the postnatal development of the pineal gland in the golden hamster (Mesocricetus auratus). On day 4 post partum, synaptic ribbons appear in cells that have already started to differentiate into pinealocytes. Between days 4 and 9, an increase in the number of synaptic ribbons occurs, concomitant with the continuing differentiation of the pineal tissue. Between days 9 and 16, when differentiation of this tissue is almost completed, the number of synaptic ribbons decreases and approaches that characteristic of the adult pineal gland. During development, the synaptic ribbons increase in length, and dense core vesicles are frequently found in the vicinity of these structures. It is assumed that a functional relationship exists between dense core vesicles and the synaptic ribbons, which are considered to be engaged in a certain form of secretory activity of the mammalian pineal gland."}
{"id": "PMID:476809", "title": "Effect of estradiol-17 beta on the cytology of the liver, gonads and pituitary, and on plasma electrolytes in the female freshwater eel.", "content": "Female freshwater eels injected with estradiol-17 beta (E2) for 15--78 days appear paler and secrete more mucus than controls. The resulting strongly opalescent blood plasma indicates that vitellogenin synthesis occurs in the liver, which shows a significant hypertrophy, an increased vacuolization (lipid material) and glycogen depletion. Plasma sodium is lowered, but calcium levels are considerably increased. The gonosomatic index increases (0.92 +/- 0.1 to a maximum of 2.21). Oocytes are enlarged, but the incorporation of vitellogenin remains discrete. Gonadotrophs (GTH cells), small and scarcely visible in the pituitary of control eels, are hypertrophied and contain numerous glycoprotein granules after E2-administration. E2 may act on the pituitary and/or hypothalamus via a positive feedback to induce gonadotrophin (GTH) synthesis; GTH release seems to be very limited as indicated by the ovarian response. The differentiation of GTH cells in eels treated with fish pituitary extracts is most probably due to secretion of E2 by the ovary, which reacts on the pituitary. Various hypotheses are considered to explain the low GTH release. Thyrotrophs, somatotrophs and prolactin cells of the pituitary are stimulated. In the pars intermedia, MSH and PAS-positive cells appear less active. A possible antidopaminergic effect of E2 is discussed. E2 administration constitutes a simple and economic technique to induce the synthesis of GTH and will facilitate the biochemical and biological study of the latter hormone in eels.", "contents": "Effect of estradiol-17 beta on the cytology of the liver, gonads and pituitary, and on plasma electrolytes in the female freshwater eel. Female freshwater eels injected with estradiol-17 beta (E2) for 15--78 days appear paler and secrete more mucus than controls. The resulting strongly opalescent blood plasma indicates that vitellogenin synthesis occurs in the liver, which shows a significant hypertrophy, an increased vacuolization (lipid material) and glycogen depletion. Plasma sodium is lowered, but calcium levels are considerably increased. The gonosomatic index increases (0.92 +/- 0.1 to a maximum of 2.21). Oocytes are enlarged, but the incorporation of vitellogenin remains discrete. Gonadotrophs (GTH cells), small and scarcely visible in the pituitary of control eels, are hypertrophied and contain numerous glycoprotein granules after E2-administration. E2 may act on the pituitary and/or hypothalamus via a positive feedback to induce gonadotrophin (GTH) synthesis; GTH release seems to be very limited as indicated by the ovarian response. The differentiation of GTH cells in eels treated with fish pituitary extracts is most probably due to secretion of E2 by the ovary, which reacts on the pituitary. Various hypotheses are considered to explain the low GTH release. Thyrotrophs, somatotrophs and prolactin cells of the pituitary are stimulated. In the pars intermedia, MSH and PAS-positive cells appear less active. A possible antidopaminergic effect of E2 is discussed. E2 administration constitutes a simple and economic technique to induce the synthesis of GTH and will facilitate the biochemical and biological study of the latter hormone in eels."}
{"id": "PMID:476812", "title": "Effects  of pimozide on the ultrastructure of the pars distalis in the rat.", "content": "The effects of pimozide, a dopamine receptor-blocking agent, were studied in the pars distalis of the rat. The animals received 100 micrograms/100 g pimozide daily for 5, 10, 15, and 20 days. Pimozide induces striking ultrastructural changes after 5 days of treatment. The number of luteotroph (LTH) cells is signficantly increased; they display characteristics of stimulation. The extrusion of granules into the intercellular space via exocytosis is frequently observed. The intercellular spaces are highly dilated, forming a lacunar system filled with an amorphous material, erythrocytes and involuted LTH cells. Transitional stages in the process of involution are observed in LTH cells. Luteotroph cells also form a syncytium. Twenty days after treatment the above-described changes decrease in magnitude. The present findings suggest that pimozide stimulates the mechanism of synthesis and release in the luteotroph cells, an effect that is less evident with longer treatment.", "contents": "Effects  of pimozide on the ultrastructure of the pars distalis in the rat. The effects of pimozide, a dopamine receptor-blocking agent, were studied in the pars distalis of the rat. The animals received 100 micrograms/100 g pimozide daily for 5, 10, 15, and 20 days. Pimozide induces striking ultrastructural changes after 5 days of treatment. The number of luteotroph (LTH) cells is signficantly increased; they display characteristics of stimulation. The extrusion of granules into the intercellular space via exocytosis is frequently observed. The intercellular spaces are highly dilated, forming a lacunar system filled with an amorphous material, erythrocytes and involuted LTH cells. Transitional stages in the process of involution are observed in LTH cells. Luteotroph cells also form a syncytium. Twenty days after treatment the above-described changes decrease in magnitude. The present findings suggest that pimozide stimulates the mechanism of synthesis and release in the luteotroph cells, an effect that is less evident with longer treatment."}
{"id": "PMID:476813", "title": "A combined horseradish peroxidase and Golgi study on the afferent connections of the ventrobasal complex of the thalamus in the cat.", "content": "Afferent connections to the ventrobasal complex (VB) of the thalamus were studied by means of retrograde transport of horseradish peroxidase (HRP) and by the Golgi-method. After HRP-injection into the VB, peroxidase-positive cells were observed contralaterally in the dorsal column nuclei (DCN), in the trigeminal nuclei and in the lateral cervical nucleus (LCN), and ipsilaterally in the somatosensoty I (SI) and II (SII) cortical areas. Labeled cells of different shape and size were compared with neurons impregnated by the Golgi-technique. On tn size and shape even within one region and that they correspond to the relay or efferent neurons observed in the Golgi-material.", "contents": "A combined horseradish peroxidase and Golgi study on the afferent connections of the ventrobasal complex of the thalamus in the cat. Afferent connections to the ventrobasal complex (VB) of the thalamus were studied by means of retrograde transport of horseradish peroxidase (HRP) and by the Golgi-method. After HRP-injection into the VB, peroxidase-positive cells were observed contralaterally in the dorsal column nuclei (DCN), in the trigeminal nuclei and in the lateral cervical nucleus (LCN), and ipsilaterally in the somatosensoty I (SI) and II (SII) cortical areas. Labeled cells of different shape and size were compared with neurons impregnated by the Golgi-technique. On tn size and shape even within one region and that they correspond to the relay or efferent neurons observed in the Golgi-material."}
{"id": "PMID:476814", "title": "Cytological changes in the pars distalis of the female rat hypophysis grafted under the kidney capsule.", "content": "Intact female rats received transplants of two hypophysial partes distales under the kidney capsule. The plasma levels of prolactin were determined, and the ultrastructure of the grafted gland was studied 15, 45 and 90 days after the operation. Although prolactin levels in the three experimental groups were significantly higher than those in control rats, a decrease in prolactin level was detected in the 45-day samples. Parallel ultrastructural changes suggest that between the 45th and 90th postoperative day a process is initiated leading to hyperplasia and hypertrophy of prolactotrophs.", "contents": "Cytological changes in the pars distalis of the female rat hypophysis grafted under the kidney capsule. Intact female rats received transplants of two hypophysial partes distales under the kidney capsule. The plasma levels of prolactin were determined, and the ultrastructure of the grafted gland was studied 15, 45 and 90 days after the operation. Although prolactin levels in the three experimental groups were significantly higher than those in control rats, a decrease in prolactin level was detected in the 45-day samples. Parallel ultrastructural changes suggest that between the 45th and 90th postoperative day a process is initiated leading to hyperplasia and hypertrophy of prolactotrophs."}
{"id": "PMID:476826", "title": "Teratocarcinoma stem cells have a cell surface carbohydrate-binding component implicated in cell-cell adhesion.", "content": "Teratocarcinoma stem cells maintained in the undifferentiated state express a carbohydrate-binding component that recognizes oligomannosyl residues. This cell surface molecule is detected by a rosetta assay in which the stem cells form rosettes with glutaraldehyde-fixed trypsinized rabbit erythrocytes. Addition of simple sugars to the assay mixture has little effect, but rosette formation is inhibited by a series of mannose-rich glycoproteins (yeast invertase, yeast mannans and horseradish peroxidase). Periodate oxidation eliminates the inhibitory activity of invertase whereas pronase digestion has little effect, indicating that carbohydrate moieties are essential for inhibition. Invertase and its glycopeptide derivatives also inhibit the reaggregation of dispersed stem cells and promote the dissociation of preformed aggregates. These results suggest that intercellular adhesion of teratocarcinoma stem cels may be the consequence of the interaction of a lectin-like component detected in the rosette assay with a complementary oligosaccharide receptor on adjacent cells.", "contents": "Teratocarcinoma stem cells have a cell surface carbohydrate-binding component implicated in cell-cell adhesion. Teratocarcinoma stem cells maintained in the undifferentiated state express a carbohydrate-binding component that recognizes oligomannosyl residues. This cell surface molecule is detected by a rosetta assay in which the stem cells form rosettes with glutaraldehyde-fixed trypsinized rabbit erythrocytes. Addition of simple sugars to the assay mixture has little effect, but rosette formation is inhibited by a series of mannose-rich glycoproteins (yeast invertase, yeast mannans and horseradish peroxidase). Periodate oxidation eliminates the inhibitory activity of invertase whereas pronase digestion has little effect, indicating that carbohydrate moieties are essential for inhibition. Invertase and its glycopeptide derivatives also inhibit the reaggregation of dispersed stem cells and promote the dissociation of preformed aggregates. These results suggest that intercellular adhesion of teratocarcinoma stem cels may be the consequence of the interaction of a lectin-like component detected in the rosette assay with a complementary oligosaccharide receptor on adjacent cells."}
{"id": "PMID:476827", "title": "Fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts.", "content": "The state of chick embryo chondroblasts in culture was found to be sensitive to both fibronectin and another substance(s) (activity A) which could be extracted from chick embryo fibroblasts with 1 M urea or from conditioned medium. In the presence of either of these activities at concentrations of 25-150 micrograms/ml, chondroblasts, which normally grow as mixed cultures of floating and adherent cells, all immediately became attached to the tissue culture dish and spread. After several days, the morphology of these typically epithelioid cells became fibroblastic. This did not involve a selection process, since the effect was reversible. The synthetic program of these cells was also dramatically modified: the cultures no longer synthesized the chondroblast-unique type IV sulfated proteoglycan and began synthesizing alpha 2 collagen chains typical of fibroblastic or early limb bud cells. Fibronectin was resolved from activity A by gelatin affinity chromatography or gel filtration. Both activities were trypsin-sensitive. The two activities differed, however, on the basis of how the protein fractions in which they were found migrated in SDS-polyacrylamide gels, their specific activities and their effects on cell morphology and cell growth.", "contents": "Fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts. The state of chick embryo chondroblasts in culture was found to be sensitive to both fibronectin and another substance(s) (activity A) which could be extracted from chick embryo fibroblasts with 1 M urea or from conditioned medium. In the presence of either of these activities at concentrations of 25-150 micrograms/ml, chondroblasts, which normally grow as mixed cultures of floating and adherent cells, all immediately became attached to the tissue culture dish and spread. After several days, the morphology of these typically epithelioid cells became fibroblastic. This did not involve a selection process, since the effect was reversible. The synthetic program of these cells was also dramatically modified: the cultures no longer synthesized the chondroblast-unique type IV sulfated proteoglycan and began synthesizing alpha 2 collagen chains typical of fibroblastic or early limb bud cells. Fibronectin was resolved from activity A by gelatin affinity chromatography or gel filtration. Both activities were trypsin-sensitive. The two activities differed, however, on the basis of how the protein fractions in which they were found migrated in SDS-polyacrylamide gels, their specific activities and their effects on cell morphology and cell growth."}
{"id": "PMID:476828", "title": "Abnormal lipid-linked oligosaccharides in class E Thy-1-negative mutant lymphomas.", "content": "The glycosylation defect of Thy-1-mutant lymphomas of the class E complementation group has been identified as a block in the synthesis of the lipid-linked oligosaccharide precursor of the asparagine-linked oligosaccharides of glycoproteins. Two major lipid-linked oligosaccharides were isolated from the mutant cells. Both oligosaccharides were smaller than the lipid-linkid oligosaccharides of wild-type lymphomas and, in contrast to the lipid-linked oligosaccharides isolated from wild-type cells, both were resistant to digestion with endoglycosidase H. The oligosaccharides of newly synthesized polypeptides in class E Thy-1-cells were also resistant to endoglycosidase H digestion, providing strong evidence that they are derived from the abnormal lipid-linked oligosaccharides.", "contents": "Abnormal lipid-linked oligosaccharides in class E Thy-1-negative mutant lymphomas. The glycosylation defect of Thy-1-mutant lymphomas of the class E complementation group has been identified as a block in the synthesis of the lipid-linked oligosaccharide precursor of the asparagine-linked oligosaccharides of glycoproteins. Two major lipid-linked oligosaccharides were isolated from the mutant cells. Both oligosaccharides were smaller than the lipid-linkid oligosaccharides of wild-type lymphomas and, in contrast to the lipid-linked oligosaccharides isolated from wild-type cells, both were resistant to digestion with endoglycosidase H. The oligosaccharides of newly synthesized polypeptides in class E Thy-1-cells were also resistant to endoglycosidase H digestion, providing strong evidence that they are derived from the abnormal lipid-linked oligosaccharides."}
{"id": "PMID:476830", "title": "Export of proteins from oocytes of Xenopus laevis.", "content": "When human lymphoblastoid mRNA was microinjected into X. laevis oocytes, titers of interferon rapidly reached a maximum inside the oocyte while accumulation of interferon continued in the incubation medium for at least 45 hr. If interferon protein was injected into oocytes it was rapidly inactivated. Significantly, newly synthesized interferon but not injected interferon was found to be membrane-associated. Further experiments involving the co-injection of mRNAs coding for secretory proteins (guinea pig milk proteins and human interferon) and nonsecretory proteins (rabbit globin) revealed that only the secretory proteins were exported from the oocyte. Moreover, different proteins were exported at different rates. A distinct subclass of newly synthesized oocyte proteins of unknown function also accumulated in the incubation medium. Since the information encoded in the messenger RNAs of secretory proteins is sufficient to specify synthesis, compartmentation and secretion of these proteins, the oocyte may provide a complete system for the analysis of the secretory process.", "contents": "Export of proteins from oocytes of Xenopus laevis. When human lymphoblastoid mRNA was microinjected into X. laevis oocytes, titers of interferon rapidly reached a maximum inside the oocyte while accumulation of interferon continued in the incubation medium for at least 45 hr. If interferon protein was injected into oocytes it was rapidly inactivated. Significantly, newly synthesized interferon but not injected interferon was found to be membrane-associated. Further experiments involving the co-injection of mRNAs coding for secretory proteins (guinea pig milk proteins and human interferon) and nonsecretory proteins (rabbit globin) revealed that only the secretory proteins were exported from the oocyte. Moreover, different proteins were exported at different rates. A distinct subclass of newly synthesized oocyte proteins of unknown function also accumulated in the incubation medium. Since the information encoded in the messenger RNAs of secretory proteins is sufficient to specify synthesis, compartmentation and secretion of these proteins, the oocyte may provide a complete system for the analysis of the secretory process."}
{"id": "PMID:476832", "title": "Morphogenesis in Volvox: analysis of critical variables.", "content": "Inversion, the process by which Volvox embryos turn inside out, was analyzed by a combination of geometrical and experimental techniques. It was shown that simple geometric figures are adequate to represent cell shapes during inversion and that cell volumes remain constant as cell shapes change and the embryo inverts. The first stage of inversion, phialopore opening, results from the release of compressive forces as the embryo withdraws from its surrounding vesicle during a two-stage contraction of each cell around its radial axis. Premature phialopore opening occurs when withdrawal of the embryo from the vesicle is elicited artificially by exposure to either calcium ionophore or hypertonic solutions. The major event of inversion, generation of negative curvature, requires both microtubule-driven elongation of cells (to produce a classical \"flask\" shape) and cytochalasin-sensitive active migration of cytoplasmic bridges to the outermost ends of flask cells. Colchicine, cyclic GMP and isobutyl methyl xanthine (individually) block both normal elongation and bridge migration; cytochalasin D blocks bridge migration selectively. Flask cell formation and bridge migration are adequate to account for the negative curvature observed. An asymmetric bending of flask cell stalks along the ring of maximum curvature accounts for the fact that the embryo is not constricted in a \"purse-string\" fashion as negative curvature is generated. Inversion of the posterior hemisphere involves an elastic snap-through resulting from a combination of compressive stresses generated by inversion of the anterior hemisphere and the circumferential restraint imposed by cells at the equator. We conclude that the observed changes in cell shape and the migration of cytoplasmic bridges are the result of an ordered process of membrane-cytoskeletal interactions, and both necessary and sufficient to account for the morphogenetic process of inversion in Volvox.", "contents": "Morphogenesis in Volvox: analysis of critical variables. Inversion, the process by which Volvox embryos turn inside out, was analyzed by a combination of geometrical and experimental techniques. It was shown that simple geometric figures are adequate to represent cell shapes during inversion and that cell volumes remain constant as cell shapes change and the embryo inverts. The first stage of inversion, phialopore opening, results from the release of compressive forces as the embryo withdraws from its surrounding vesicle during a two-stage contraction of each cell around its radial axis. Premature phialopore opening occurs when withdrawal of the embryo from the vesicle is elicited artificially by exposure to either calcium ionophore or hypertonic solutions. The major event of inversion, generation of negative curvature, requires both microtubule-driven elongation of cells (to produce a classical \"flask\" shape) and cytochalasin-sensitive active migration of cytoplasmic bridges to the outermost ends of flask cells. Colchicine, cyclic GMP and isobutyl methyl xanthine (individually) block both normal elongation and bridge migration; cytochalasin D blocks bridge migration selectively. Flask cell formation and bridge migration are adequate to account for the negative curvature observed. An asymmetric bending of flask cell stalks along the ring of maximum curvature accounts for the fact that the embryo is not constricted in a \"purse-string\" fashion as negative curvature is generated. Inversion of the posterior hemisphere involves an elastic snap-through resulting from a combination of compressive stresses generated by inversion of the anterior hemisphere and the circumferential restraint imposed by cells at the equator. We conclude that the observed changes in cell shape and the migration of cytoplasmic bridges are the result of an ordered process of membrane-cytoskeletal interactions, and both necessary and sufficient to account for the morphogenetic process of inversion in Volvox."}
{"id": "PMID:476833", "title": "Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.", "content": "A series of adenovirus type 5 (Ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. The mutants were selected as variants which lack the Xba I endonuclease cleavage site at 4 map units on the viral chromosome. The deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. The mutants can be propagated in 293 cells (Ad5-transformed human embryonic kidney cells), but are defective for growth in HeLa or human embryonic kidney cells. No viral DNA synthesis was observed in mutant virus-infected HeLa cells. All but one of the deletion mutants tested were defective for transformation of rat embryo and rat embryo brain cells.", "contents": "Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. A series of adenovirus type 5 (Ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. The mutants were selected as variants which lack the Xba I endonuclease cleavage site at 4 map units on the viral chromosome. The deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. The mutants can be propagated in 293 cells (Ad5-transformed human embryonic kidney cells), but are defective for growth in HeLa or human embryonic kidney cells. No viral DNA synthesis was observed in mutant virus-infected HeLa cells. All but one of the deletion mutants tested were defective for transformation of rat embryo and rat embryo brain cells."}
{"id": "PMID:476837", "title": "Studies on microplasmodia of Physarum polycephalum V: electrical activity of different types of microplasmodia and macroplasmodia.", "content": "Results obtained by three different electrophysiological methods delivered mainly oscillating potentials (independently of the type of organization of Physarum polycephalum) with an average period length of 1 min 21 sec. This value is in good statistical agreement with corresponding data for the periodical oscillation of the shuttle streaming (1 min 47 sec) and coincides also with the radial tensiometric contractile activity (1 min 34 sec). The investigation favours the hypothesis that ion fluxes across the cell membrane and perhaps between intracellular compartments may act as a trigger mechanism for a still unknown oscillator-system which controls cell movement phenomena in the acellular slime molds.", "contents": "Studies on microplasmodia of Physarum polycephalum V: electrical activity of different types of microplasmodia and macroplasmodia. Results obtained by three different electrophysiological methods delivered mainly oscillating potentials (independently of the type of organization of Physarum polycephalum) with an average period length of 1 min 21 sec. This value is in good statistical agreement with corresponding data for the periodical oscillation of the shuttle streaming (1 min 47 sec) and coincides also with the radial tensiometric contractile activity (1 min 34 sec). The investigation favours the hypothesis that ion fluxes across the cell membrane and perhaps between intracellular compartments may act as a trigger mechanism for a still unknown oscillator-system which controls cell movement phenomena in the acellular slime molds."}
{"id": "PMID:476838", "title": "Septate-like junctions between cells of the immune system in vivo.", "content": "Septate-like junctions between lymphocytes, lymphocytes and macrophages, and macrophages were observed in cellular capsules surrounding larvae of the tapeworm Taenia crassiceps after intraperitoneal inoculation into immunized mice. The morphology of the junctions found in vivo is similar to that reported for septate-like junctions between cells of the immune system in vitro. These findings support the possibility that septate-like junctions may be involved in functional interactions between cells of the immune system both in vivo and in vitro.", "contents": "Septate-like junctions between cells of the immune system in vivo. Septate-like junctions between lymphocytes, lymphocytes and macrophages, and macrophages were observed in cellular capsules surrounding larvae of the tapeworm Taenia crassiceps after intraperitoneal inoculation into immunized mice. The morphology of the junctions found in vivo is similar to that reported for septate-like junctions between cells of the immune system in vitro. These findings support the possibility that septate-like junctions may be involved in functional interactions between cells of the immune system both in vivo and in vitro."}
{"id": "PMID:476839", "title": "Growth in short term primary cell cultures derived from pregnancy-dependent mammary tumors.", "content": "The behavior of cells in primary cultures derived from autonomous and pregnancy-dependent mouse mammary tumors was studied. Despite initial growth both dependent and autonomous mammary tumors produced only short-term primary cultures. Initial plating density had a marked effect on growth with only cultures plated at greater than or equal to 2 X 10(5) cells/cm2 showing any short-termed growth. Time lapse analysis showed that the lack of growth was due to failures of cytokinesis and increased death rate and intermitotic time in cultures plated at less than 2 X 10(5) cells/cm2. Using continuous label autoradiographic techniques, a partial synchronous wave of DNA synthesis was observed with newly plated and restimulated cultures. DNA synthesis reached a peak 24-48 hrs. after plating or restimulation and then dropped to low values for the next few days. Attempts to maintain the initial high rate of DNA synthesis or to induce another round of DNA synthesis by enriched media, increased serum concentrations, or other types of serum and plasma were at best only partially successful. Important hormones necessary for growth of mammary tissue in vivo may be necessary for sustained growth in vitro.", "contents": "Growth in short term primary cell cultures derived from pregnancy-dependent mammary tumors. The behavior of cells in primary cultures derived from autonomous and pregnancy-dependent mouse mammary tumors was studied. Despite initial growth both dependent and autonomous mammary tumors produced only short-term primary cultures. Initial plating density had a marked effect on growth with only cultures plated at greater than or equal to 2 X 10(5) cells/cm2 showing any short-termed growth. Time lapse analysis showed that the lack of growth was due to failures of cytokinesis and increased death rate and intermitotic time in cultures plated at less than 2 X 10(5) cells/cm2. Using continuous label autoradiographic techniques, a partial synchronous wave of DNA synthesis was observed with newly plated and restimulated cultures. DNA synthesis reached a peak 24-48 hrs. after plating or restimulation and then dropped to low values for the next few days. Attempts to maintain the initial high rate of DNA synthesis or to induce another round of DNA synthesis by enriched media, increased serum concentrations, or other types of serum and plasma were at best only partially successful. Important hormones necessary for growth of mammary tissue in vivo may be necessary for sustained growth in vitro."}
{"id": "PMID:476841", "title": "Polyadenylate-protein complexes in resting and growing 3T3 cells.", "content": "The properties of the ribonuclease resistant cytoplasmic ribonucleoprotein particles were studied in contact-inhibited and serum induced proliferating 3T3 cells. The RNP particles were fractionated by oligo (dT)-cellulose chromatography and banded in CsSO4 gradients. The main RNP fraction, eluted with 25% formamide, contained the major ribonuclease resistant RNA sequences in both resting and growing cells. The protein component of this fraction had a molecular weight of about 72,000 in contact-inhibited cells and 81,000 in serum induced cells.", "contents": "Polyadenylate-protein complexes in resting and growing 3T3 cells. The properties of the ribonuclease resistant cytoplasmic ribonucleoprotein particles were studied in contact-inhibited and serum induced proliferating 3T3 cells. The RNP particles were fractionated by oligo (dT)-cellulose chromatography and banded in CsSO4 gradients. The main RNP fraction, eluted with 25% formamide, contained the major ribonuclease resistant RNA sequences in both resting and growing cells. The protein component of this fraction had a molecular weight of about 72,000 in contact-inhibited cells and 81,000 in serum induced cells."}
{"id": "PMID:476840", "title": "Effect of hormones on primary cell cultures from pregnancy-dependent mouse mammary tumors.", "content": "No hormone combination was successful in maintaining long term primary cultures of pregnancy-dependent mammary tumors. Insulin provided the most consistent dose dependent stimulatory effect on short term proliferation as measured by 3H-TdR incorporation into DNA. Insulin in combination with corticosterone and prolactin produced the greatest stimulatory effect in most tumors. Both insulin at 5 microgram/ml and insulin, prolactin and hydrocortisone induced a partially synchronous wave of DNA synthesis in restimulated cultures. The time course of the wave of DNA synthesis was different for different hormone treatments. Insulin as 5 microgram/ml caused an earlier wave of DNA synthesis than insulin, prolactin plus corticosterone.", "contents": "Effect of hormones on primary cell cultures from pregnancy-dependent mouse mammary tumors. No hormone combination was successful in maintaining long term primary cultures of pregnancy-dependent mammary tumors. Insulin provided the most consistent dose dependent stimulatory effect on short term proliferation as measured by 3H-TdR incorporation into DNA. Insulin in combination with corticosterone and prolactin produced the greatest stimulatory effect in most tumors. Both insulin at 5 microgram/ml and insulin, prolactin and hydrocortisone induced a partially synchronous wave of DNA synthesis in restimulated cultures. The time course of the wave of DNA synthesis was different for different hormone treatments. Insulin as 5 microgram/ml caused an earlier wave of DNA synthesis than insulin, prolactin plus corticosterone."}
{"id": "PMID:476852", "title": "Antithyroid and antiperoxidase activity of tropolone and 3-hydroxy-4-pyrone.", "content": "Tropolone (TR) and 3-hydroxy-4-pyrone were investigated for antithyroid activity following the finding that the 2-hydroxy-oxo pyridine, 3-hydroxy-4(1H)-pyridone (DHP, I), is goitrogenic. Both compounds inhibited the thyroidal uptake of radioiodine in rats and resembled the thioamide drugs in inhibiting the organic binding of iodine by the thyroid gland rather than the trapping of iodide, but were weaker binding inhibitors than 6-methyl-2-thiouracil (MeTU). Both compounds also inhibited the iodination of bovine serum albumin and thyroglobulin, catalyzed by thyroidperoxidase (TPO), lactoperoxidase (LPO), chloroperoxidase (CPO) and horseradish peroxidase (HPO) in vitro. The inhibitory effect of TR but not that of 3-hydroxy-4-pyrone was antagonized by ferrous ions. When fed to mice at levels of intake expected to produce goitre both compounds were toxic and caused severe liver damage. Thyroid enlargement was not observed in any of these feeiding experiments, but the thyroids of mice fed 0.1% TR showed moderate hyperplasia. It was concluded that both compounds are weakly goitrogenic. Hyperactivity was observed in the mice fed TR which may be associated with inhibition of catechol methyl transferase (COMT).", "contents": "Antithyroid and antiperoxidase activity of tropolone and 3-hydroxy-4-pyrone. Tropolone (TR) and 3-hydroxy-4-pyrone were investigated for antithyroid activity following the finding that the 2-hydroxy-oxo pyridine, 3-hydroxy-4(1H)-pyridone (DHP, I), is goitrogenic. Both compounds inhibited the thyroidal uptake of radioiodine in rats and resembled the thioamide drugs in inhibiting the organic binding of iodine by the thyroid gland rather than the trapping of iodide, but were weaker binding inhibitors than 6-methyl-2-thiouracil (MeTU). Both compounds also inhibited the iodination of bovine serum albumin and thyroglobulin, catalyzed by thyroidperoxidase (TPO), lactoperoxidase (LPO), chloroperoxidase (CPO) and horseradish peroxidase (HPO) in vitro. The inhibitory effect of TR but not that of 3-hydroxy-4-pyrone was antagonized by ferrous ions. When fed to mice at levels of intake expected to produce goitre both compounds were toxic and caused severe liver damage. Thyroid enlargement was not observed in any of these feeiding experiments, but the thyroids of mice fed 0.1% TR showed moderate hyperplasia. It was concluded that both compounds are weakly goitrogenic. Hyperactivity was observed in the mice fed TR which may be associated with inhibition of catechol methyl transferase (COMT)."}
{"id": "PMID:476851", "title": "Accumulation of cadmium and lead in the gills of Mytilus edulis: X-ray microanalysis and chemical analysis.", "content": "The accumulation of Cd and Pb in the gills of the lamellibranch mollusc Mytilus edulis has been studied by electron microscopy, X-ray microanalysis, atomic absorption spectroscopy and radionuclide monitoring. The patterns of accumulation of the two elements differ markedly as do the sites of deposition whithin the gills. Lead is found extracellularly as crystalline deposits in the basal lamina which forms the capillary walls of the gill lamellae. The Pb is found associated with Ca in equiatomic ratios and occurs either as a mixed or complex carbonate. Cadmium is always associated with S and frequently with P in membrane bound vesicles within the cells of the gill epithelium and in the amoebocytes. The S is probably attributable to the presence of cysteine residues in a metal binding protein which can be extracted from the gills. Analysis of the metal binding protein shows that it binds Ag, Cd, Cu, Fe, Hg, Sn and Zn. Its amino acid composition is similar to that reported for eels and limpets but has a lower cysteine content than mammalian metal binding protein.", "contents": "Accumulation of cadmium and lead in the gills of Mytilus edulis: X-ray microanalysis and chemical analysis. The accumulation of Cd and Pb in the gills of the lamellibranch mollusc Mytilus edulis has been studied by electron microscopy, X-ray microanalysis, atomic absorption spectroscopy and radionuclide monitoring. The patterns of accumulation of the two elements differ markedly as do the sites of deposition whithin the gills. Lead is found extracellularly as crystalline deposits in the basal lamina which forms the capillary walls of the gill lamellae. The Pb is found associated with Ca in equiatomic ratios and occurs either as a mixed or complex carbonate. Cadmium is always associated with S and frequently with P in membrane bound vesicles within the cells of the gill epithelium and in the amoebocytes. The S is probably attributable to the presence of cysteine residues in a metal binding protein which can be extracted from the gills. Analysis of the metal binding protein shows that it binds Ag, Cd, Cu, Fe, Hg, Sn and Zn. Its amino acid composition is similar to that reported for eels and limpets but has a lower cysteine content than mammalian metal binding protein."}
{"id": "PMID:476853", "title": "Mutagenicity testing on chinese hamster V79 cells treated in the in vitro liver perfusion system. Comparative investigation of different in vitro metabolising systems with dimethylnitrosamine and benzo[a]pyrene.", "content": "A comparative study of three in vitro metabolising systems was performed in combination with Chinese hamster V79 cells, at which point mutation to 6-thioguanine resistance was scored. The three metabolising systems used were: (1) rat liver microsomal fraction (S9-mix); (2) feeder layer of primary embryonic golden hamster cells, according to Hubermann's system; (3) in vitro perfusion of rat liver according to the system of Beije et al. As model substances dimethylnitrosamine (DMN) and benzo[a]pyrene (BP) was used. The liver perfusion was more efficient than S9-mix as an activating system of DMN, while the feeder layer of embryonic cells was unable to activate this compound. The activation of DMN with S9-mix was dependent on the presence of NADP. By exposing the target cells in the liver perfusion at different distances from the liver the biological half life of the active metabolite of DMN could be estimated to less than 5 s. With BP the three metabolising systems showed reversed results as compared with DMN--both the feeder layer cells and S9-mix activated BP, the feeder layer cells being most efficient. With liver perfusion, the perfusate itself was totally negative. Only the bile showed a week mutagenic effect. These results are in accordance with the notion that intact liver cells perform both an activation and a subsequent deactivation of BP. Because of the importance of hepatic bio-transformation in chemical mutagenesis and carcinogenesis it is emphasied that a liver perfusion system could be used in a testing protocol for genotoxic effects as a valuable tool in order to analyse the mechanism of action of mutagenic and carcinogenic compounds detected in other test systems, for instance bacterial/microsomal tests.", "contents": "Mutagenicity testing on chinese hamster V79 cells treated in the in vitro liver perfusion system. Comparative investigation of different in vitro metabolising systems with dimethylnitrosamine and benzo[a]pyrene. A comparative study of three in vitro metabolising systems was performed in combination with Chinese hamster V79 cells, at which point mutation to 6-thioguanine resistance was scored. The three metabolising systems used were: (1) rat liver microsomal fraction (S9-mix); (2) feeder layer of primary embryonic golden hamster cells, according to Hubermann's system; (3) in vitro perfusion of rat liver according to the system of Beije et al. As model substances dimethylnitrosamine (DMN) and benzo[a]pyrene (BP) was used. The liver perfusion was more efficient than S9-mix as an activating system of DMN, while the feeder layer of embryonic cells was unable to activate this compound. The activation of DMN with S9-mix was dependent on the presence of NADP. By exposing the target cells in the liver perfusion at different distances from the liver the biological half life of the active metabolite of DMN could be estimated to less than 5 s. With BP the three metabolising systems showed reversed results as compared with DMN--both the feeder layer cells and S9-mix activated BP, the feeder layer cells being most efficient. With liver perfusion, the perfusate itself was totally negative. Only the bile showed a week mutagenic effect. These results are in accordance with the notion that intact liver cells perform both an activation and a subsequent deactivation of BP. Because of the importance of hepatic bio-transformation in chemical mutagenesis and carcinogenesis it is emphasied that a liver perfusion system could be used in a testing protocol for genotoxic effects as a valuable tool in order to analyse the mechanism of action of mutagenic and carcinogenic compounds detected in other test systems, for instance bacterial/microsomal tests."}
{"id": "PMID:476854", "title": "Effect of a single treatment with the alkylating carcinogens dimethylnitrosamine and methyl methanesulphonate on liver regenerating after partial hepatectomy. IV. Effect on methylase-mediated methylation of DNA.", "content": "The possibility that carcinogens may affect methylase-mediated methylation of replicating DNA was investigated. A system eminently suitable for this purpose is liver regenerating after partial hepatectomy, as one injection of dimethylnitrosamine (DMN) given during the ensuing period of increased DNA synthesis induces hepatocellular carcinoma. Methylation of DNA by DNA methylase normally occurs only in proportion to DNA synthesis. Therefore simultaneous measurements were made of synthesis (incorporation of [14C]adenine into DNA adenine, or of d[5-3H]cytidine into DNA cytosine), and of methylation (incorporation of [methyl-3H]methionine into 5-methylcytosine of DNA) in liver regenerating after partial hepatectomy. After treatment with DMN, the ratio of methylation: synthesis remained within the normal range. Methyl methanesulphonate (MMS), a compound which damages DNA in regenerating liver in a similar but not identical way to DMN and which does not induce tumors in liver even when given after partial hepatectomy, caused an increase in methylation in relation to synthesis. These experiments therefore do not support the view that altered DNA methylase activity is involved in carcinogenesis.", "contents": "Effect of a single treatment with the alkylating carcinogens dimethylnitrosamine and methyl methanesulphonate on liver regenerating after partial hepatectomy. IV. Effect on methylase-mediated methylation of DNA. The possibility that carcinogens may affect methylase-mediated methylation of replicating DNA was investigated. A system eminently suitable for this purpose is liver regenerating after partial hepatectomy, as one injection of dimethylnitrosamine (DMN) given during the ensuing period of increased DNA synthesis induces hepatocellular carcinoma. Methylation of DNA by DNA methylase normally occurs only in proportion to DNA synthesis. Therefore simultaneous measurements were made of synthesis (incorporation of [14C]adenine into DNA adenine, or of d[5-3H]cytidine into DNA cytosine), and of methylation (incorporation of [methyl-3H]methionine into 5-methylcytosine of DNA) in liver regenerating after partial hepatectomy. After treatment with DMN, the ratio of methylation: synthesis remained within the normal range. Methyl methanesulphonate (MMS), a compound which damages DNA in regenerating liver in a similar but not identical way to DMN and which does not induce tumors in liver even when given after partial hepatectomy, caused an increase in methylation in relation to synthesis. These experiments therefore do not support the view that altered DNA methylase activity is involved in carcinogenesis."}
{"id": "PMID:476855", "title": "Altered RNA transport without derepression in a rat kidney tumor induced by dimethylnitrosamine.", "content": "RNA/DNA hybridization in the presence of competing RNA was used to study transcription and transport of RNA from the nucleus to the cytoplasm in a rat renal tumor induced by a single intraperitoneal injection of dimethylnitrosamine (DMN). Neither repression nor derepression of transcription of repetitive DNA can be detected in this tumor, but transcripts of all active families of such sequences are found in the tumor cytoplasm. This is the pattern of RNA change found in the chemically-induced hepatocellular carcinomas and contrasts with the pattern of virus-induced renal tumors in which both altered transport and derepression of host repetitive sequences have been demonstrated. The alteration of RNA transport is nearly complete in the kidney only two days after the above treatment, but there is no such alteration detectable in the kidney after nine days of feeding a liver carcinogen which is not carcinogenic to kidneys. This data supports the significance and specificity of the loss of ability to restrict certain RNA sequences to the nucleus early during malignant transformation.", "contents": "Altered RNA transport without derepression in a rat kidney tumor induced by dimethylnitrosamine. RNA/DNA hybridization in the presence of competing RNA was used to study transcription and transport of RNA from the nucleus to the cytoplasm in a rat renal tumor induced by a single intraperitoneal injection of dimethylnitrosamine (DMN). Neither repression nor derepression of transcription of repetitive DNA can be detected in this tumor, but transcripts of all active families of such sequences are found in the tumor cytoplasm. This is the pattern of RNA change found in the chemically-induced hepatocellular carcinomas and contrasts with the pattern of virus-induced renal tumors in which both altered transport and derepression of host repetitive sequences have been demonstrated. The alteration of RNA transport is nearly complete in the kidney only two days after the above treatment, but there is no such alteration detectable in the kidney after nine days of feeding a liver carcinogen which is not carcinogenic to kidneys. This data supports the significance and specificity of the loss of ability to restrict certain RNA sequences to the nucleus early during malignant transformation."}
{"id": "PMID:476867", "title": "The effect of fluid volume loading on exclusion of interstitial albumin and lymph flow in the dog lung.", "content": "The excluded volume fraction for interstitial albumin (FE) was estimated in the lungs of seven mongrel dogs during steady state conditions following intravenous infusions of Ringer's solution, amounting to 0, 5, 10, and 15% of body weight (BW). We estimated the tissue blood volume with 51Cr red cells, extracellular space with 99mTc-diethylenetriaminepentaacetic acid (99mTc-DTPA), and the albumin pool with 125I human serum albumin. A prenodal tracheobronchial lymphatic was cannulated for recording lymph flow (QL), total protein (CL), and albumin [CL(A)] concentrations. From these measurements, we calculated the extravascular albumin content (QA) and 99mTc-DTPA space (VI) of lung tissue samples collected at the successive volume expansions. The apparent tissue concentration of albumin (CApp = QA/VI) decreased from a control value (mean +/- SE) of 0.89 +/- 0.06 to 0.46 +/- 0.04 g/dl following the 15% BW infusion, whereas CL(A) decreased from 1.43 +/- 0.16 to 0.50 +/- 0.07 g/dl for the same volume expansion. By assuming that pulmonary lymph represented tissue fluid, we calculated a control FE of 0.38 +/- 0.02 using the equation, FE = 1 - [CApp/CL(A)]. FE decreased following successive infusions to 0.28 +/- 0.03, 0.16 +/- 0.02, and 0.10 +/- 0.02. These data indicate a significant contribution by the decrease in FE to the total decrease in tissue albumin concentration as interstitial fluid volume increased. Somewhat unexpectedly, the mean steady state QL increased by only 2.1-fold following the 5% BW expansion, but did not further increase following subsequent volume expansions. This has been attributed to a nonlinear interstitial compliance, sequestration of interstitial fluid, or possible deterioration of the experimental preparation.", "contents": "The effect of fluid volume loading on exclusion of interstitial albumin and lymph flow in the dog lung. The excluded volume fraction for interstitial albumin (FE) was estimated in the lungs of seven mongrel dogs during steady state conditions following intravenous infusions of Ringer's solution, amounting to 0, 5, 10, and 15% of body weight (BW). We estimated the tissue blood volume with 51Cr red cells, extracellular space with 99mTc-diethylenetriaminepentaacetic acid (99mTc-DTPA), and the albumin pool with 125I human serum albumin. A prenodal tracheobronchial lymphatic was cannulated for recording lymph flow (QL), total protein (CL), and albumin [CL(A)] concentrations. From these measurements, we calculated the extravascular albumin content (QA) and 99mTc-DTPA space (VI) of lung tissue samples collected at the successive volume expansions. The apparent tissue concentration of albumin (CApp = QA/VI) decreased from a control value (mean +/- SE) of 0.89 +/- 0.06 to 0.46 +/- 0.04 g/dl following the 15% BW infusion, whereas CL(A) decreased from 1.43 +/- 0.16 to 0.50 +/- 0.07 g/dl for the same volume expansion. By assuming that pulmonary lymph represented tissue fluid, we calculated a control FE of 0.38 +/- 0.02 using the equation, FE = 1 - [CApp/CL(A)]. FE decreased following successive infusions to 0.28 +/- 0.03, 0.16 +/- 0.02, and 0.10 +/- 0.02. These data indicate a significant contribution by the decrease in FE to the total decrease in tissue albumin concentration as interstitial fluid volume increased. Somewhat unexpectedly, the mean steady state QL increased by only 2.1-fold following the 5% BW expansion, but did not further increase following subsequent volume expansions. This has been attributed to a nonlinear interstitial compliance, sequestration of interstitial fluid, or possible deterioration of the experimental preparation."}
{"id": "PMID:476868", "title": "Evidence from bioassay studies indicating a role for adenosine in cardiac ischemic and hypoxic dilation in the dog.", "content": "Previous studies using bioassay demonstrated the presence of a vasoactive substance or substances in coronary sinus blood during myocardial reactive hyperemia which had, on bioassay, characteristics of adenosine and/or AMP. In the present studies, specific blockers were applied to an improved bioassay system to define more precisely the nature of the substance or substances and to determine whether it also appears during local cardiac hypoxia. In the anesthetized dog, coronary sinus blood was bioassayed in an isolated autologous kidney during reactive and hypoxic dilation. During reactive dilation, the bioassay kidney responded with a large resistance increase which was blocked by theophylline and adenosine deaminase and converted to a decrease by adenosine autoblockade. The same was true for hypoxic dilation, except in this instance, only adenosine deaminase reduced the response (40%). Theophylline and adenosine autoblockade eliminated responses of the bioassay organ to both exogenous adenosine and exogenous AMP, but adenosine deaminase was specific for adenosine. These results confirm that one or more vasoactive substances appear in sinus blood in vasoactive quantities during cardiac reactive dilation, and that the peak concentration correlates roughly with the peak flow. In addition, they show that this also is the case for hypoxic dilation. More importantly, they demonstrate that the substance almost certainly is adenosine in the case of reactive dilation, and that increased adenosine levels also are present during hypoxic dilation, but in addition, suggest that AMP sometimes appears in coronary sinus blood during severe cardiac hypoxia in the dog heart.", "contents": "Evidence from bioassay studies indicating a role for adenosine in cardiac ischemic and hypoxic dilation in the dog. Previous studies using bioassay demonstrated the presence of a vasoactive substance or substances in coronary sinus blood during myocardial reactive hyperemia which had, on bioassay, characteristics of adenosine and/or AMP. In the present studies, specific blockers were applied to an improved bioassay system to define more precisely the nature of the substance or substances and to determine whether it also appears during local cardiac hypoxia. In the anesthetized dog, coronary sinus blood was bioassayed in an isolated autologous kidney during reactive and hypoxic dilation. During reactive dilation, the bioassay kidney responded with a large resistance increase which was blocked by theophylline and adenosine deaminase and converted to a decrease by adenosine autoblockade. The same was true for hypoxic dilation, except in this instance, only adenosine deaminase reduced the response (40%). Theophylline and adenosine autoblockade eliminated responses of the bioassay organ to both exogenous adenosine and exogenous AMP, but adenosine deaminase was specific for adenosine. These results confirm that one or more vasoactive substances appear in sinus blood in vasoactive quantities during cardiac reactive dilation, and that the peak concentration correlates roughly with the peak flow. In addition, they show that this also is the case for hypoxic dilation. More importantly, they demonstrate that the substance almost certainly is adenosine in the case of reactive dilation, and that increased adenosine levels also are present during hypoxic dilation, but in addition, suggest that AMP sometimes appears in coronary sinus blood during severe cardiac hypoxia in the dog heart."}
{"id": "PMID:476869", "title": "Coronary vasoactivity of adenosine in the conscious dog.", "content": "Intracoronary adenosine infusions in conscious dogs produced half-maximal coronary vasodilation at 0.57 +/- 0.18 (SD) microns and at 1.01 +/- 0.25 microns in open-chest dogs. In both preparations, adenosine at concentrations in the range found in cardiac muscle by direct analysis produced coronary vasodilation equal to that attained during a maximum reactive hyperemic response. The quantitative structure-activity relationship technique was applied to data on the coronary vasoactivity of 68 adenosine analogs to identify the chemical features of this molecule that determine its vasoactivity. These are: (1) the size of the purine base; (2) the inductive effect of C-2 substituent; (3) the electron-withdrawing effect of the C-6 substituent; (4) the glycosylic torsion angle; (5) the ability of the C-2' and C-3' hydroxyls to participate in hydrogen bonding; (7) the absence of sterically hindering groups in the vicinity of C-2' and, more importantly, C-3'; and (8) the inductive effect of the C-5' substituent. The hydrophobicity of these analogs did not correlate with vasoactivity, suggesting that the hydrophilicity of the ribose moiety overshadows any hydrophobic influence of the very weakly aromatic purine base.", "contents": "Coronary vasoactivity of adenosine in the conscious dog. Intracoronary adenosine infusions in conscious dogs produced half-maximal coronary vasodilation at 0.57 +/- 0.18 (SD) microns and at 1.01 +/- 0.25 microns in open-chest dogs. In both preparations, adenosine at concentrations in the range found in cardiac muscle by direct analysis produced coronary vasodilation equal to that attained during a maximum reactive hyperemic response. The quantitative structure-activity relationship technique was applied to data on the coronary vasoactivity of 68 adenosine analogs to identify the chemical features of this molecule that determine its vasoactivity. These are: (1) the size of the purine base; (2) the inductive effect of C-2 substituent; (3) the electron-withdrawing effect of the C-6 substituent; (4) the glycosylic torsion angle; (5) the ability of the C-2' and C-3' hydroxyls to participate in hydrogen bonding; (7) the absence of sterically hindering groups in the vicinity of C-2' and, more importantly, C-3'; and (8) the inductive effect of the C-5' substituent. The hydrophobicity of these analogs did not correlate with vasoactivity, suggesting that the hydrophilicity of the ribose moiety overshadows any hydrophobic influence of the very weakly aromatic purine base."}
{"id": "PMID:476870", "title": "Sensitization of isolated canine coronary arteries to calcium ions after exposure to cholesterol.", "content": "The abundance of membrane cholesterol is an important determinant of the functional properties of biomembranes. To determine whether arterial smooth muscle acquires altered contractile properties in a high cholesterol environment, isolated canine coronary arteries were exposed to cholesterol in stable aqueous solution. Cholesterol, 10(-12) to 10(-10) M, was an efficacious vasoconstrictor, as maximum contractions equaled those obtained with 15 mM KCl. Antiadrenergic interventions, including chemical sympathectomy in vivo with 6-hydroxydopamine and alpha- and beta-adrenergic blockade with phentolamine and L-propranolol (both 10(-6 M), did not significantly attenuate the contractions. However, responses to cholesterol were abolished completely by (+/-)-verapamil (10(-6) M). Cholesterol in picomolar concentration enhanced the constrictor effects of CaCl2 and KCl, both in the presence and absence of alpha- and beta-adrenergic blockade. Increases in tone in response to graded elevations in the CaCl2 concentration (0-2 mM) were augmented up to 1.5-fold by 10(-12) M cholesterol (P less than 0.01). Results indicate that cholesterol sensitizes isolated coronary arteries to external Ca2+ by a nonadrenergic mechanism. The findings are consistent with the hypothesis that acquisition of membrane cholesterol may alter the contractile properties of coronary arterial smooth muscle, a phenomenon that could play a role in the pathophysiology of atherosclerotic heart disease.", "contents": "Sensitization of isolated canine coronary arteries to calcium ions after exposure to cholesterol. The abundance of membrane cholesterol is an important determinant of the functional properties of biomembranes. To determine whether arterial smooth muscle acquires altered contractile properties in a high cholesterol environment, isolated canine coronary arteries were exposed to cholesterol in stable aqueous solution. Cholesterol, 10(-12) to 10(-10) M, was an efficacious vasoconstrictor, as maximum contractions equaled those obtained with 15 mM KCl. Antiadrenergic interventions, including chemical sympathectomy in vivo with 6-hydroxydopamine and alpha- and beta-adrenergic blockade with phentolamine and L-propranolol (both 10(-6 M), did not significantly attenuate the contractions. However, responses to cholesterol were abolished completely by (+/-)-verapamil (10(-6) M). Cholesterol in picomolar concentration enhanced the constrictor effects of CaCl2 and KCl, both in the presence and absence of alpha- and beta-adrenergic blockade. Increases in tone in response to graded elevations in the CaCl2 concentration (0-2 mM) were augmented up to 1.5-fold by 10(-12) M cholesterol (P less than 0.01). Results indicate that cholesterol sensitizes isolated coronary arteries to external Ca2+ by a nonadrenergic mechanism. The findings are consistent with the hypothesis that acquisition of membrane cholesterol may alter the contractile properties of coronary arterial smooth muscle, a phenomenon that could play a role in the pathophysiology of atherosclerotic heart disease."}
{"id": "PMID:476871", "title": "Brain adenosine production in the rat during 60 seconds of ischemia.", "content": "In rats, cerebral perfusion pressure were altered abruptly by aortic transection to determine the production by ischemic brain of adenosine and its metabolites, inosine and hypoxanthine. Brain samples were obtained after 0, 5, 10, 15, 30, and 60 seconds of ischemia. Also measured were ATP, ADP, AMP, phosphocreatine (PCr), lactate, and pyruvate. Blood pressure was monitored continuously, and arterial PO2, PCO2, and pH were measured just prior to induction of ischemia. Adenosine was elevated t 2.30 +/- 0.31 (SE) nmol/g at 5 seconds from a control value of 0.96 +/- 0.07. A significant elevation of adenosine continued to 60 seconds (5.50 +/- 1.24). Furthermore, inosine showed a progressive upward trend during the entire 60 seconds of ischemia, whereas no change in hypoxanthine occurred between the moment of transection (31.81 +/- 2.01 nmol/g) and 60 seconds of ischemia (34.72 +/- 2.93). PCr decreased by 1.24 mumol/g within the first 5 seconds. After the onset of hypotension, significant changes did not occur in AMP and ADP until 30 seconds, and in ATP and pyruvate until 60 seconds after aortic transection; lactate was elevated by 10 seconds. The rapid rise of cerebral adenosine within 5 seconds after the onset of ischemia supports a role for adenosine in the regulation of cerebral blood flow.", "contents": "Brain adenosine production in the rat during 60 seconds of ischemia. In rats, cerebral perfusion pressure were altered abruptly by aortic transection to determine the production by ischemic brain of adenosine and its metabolites, inosine and hypoxanthine. Brain samples were obtained after 0, 5, 10, 15, 30, and 60 seconds of ischemia. Also measured were ATP, ADP, AMP, phosphocreatine (PCr), lactate, and pyruvate. Blood pressure was monitored continuously, and arterial PO2, PCO2, and pH were measured just prior to induction of ischemia. Adenosine was elevated t 2.30 +/- 0.31 (SE) nmol/g at 5 seconds from a control value of 0.96 +/- 0.07. A significant elevation of adenosine continued to 60 seconds (5.50 +/- 1.24). Furthermore, inosine showed a progressive upward trend during the entire 60 seconds of ischemia, whereas no change in hypoxanthine occurred between the moment of transection (31.81 +/- 2.01 nmol/g) and 60 seconds of ischemia (34.72 +/- 2.93). PCr decreased by 1.24 mumol/g within the first 5 seconds. After the onset of hypotension, significant changes did not occur in AMP and ADP until 30 seconds, and in ATP and pyruvate until 60 seconds after aortic transection; lactate was elevated by 10 seconds. The rapid rise of cerebral adenosine within 5 seconds after the onset of ischemia supports a role for adenosine in the regulation of cerebral blood flow."}
{"id": "PMID:476872", "title": "Sarcomere length-resting tension relation in single frog atrial cardiac cells.", "content": "It generally has been thought that the relatively high resting tension characteristic of cardiac tissue resides in structures (collagen, elastin) external to the individual cardiac cells, but the evidence to support this conclusion has been indirect, since the resting tension of intact single cardiac cells has not been determined previously. The purpose of the present investigation was to determine the resting tension (stress)-sarcomere length relationships of single intact frog atrial cells. For tension determinations, a single cell was attached between two poly-L-lysine coated glass beams; one beam served as a compliant calibrated cantilevered force beam, and length changes were imposed on the cell by movement of the other beam. Coventional bright-field light microscope techniques were used to view the cell, the sarcomere pattern within the cell, and the position of the force beam. The resting tension of the intact cell increased from a value of about 10 nN at a sarcomere length of 2.35 microns to a value of about 130 nN at a sarcomere length of 3.45 microns. Lagrangian and Eulerian resting stress-sarcomere length relationships were computed from the resting tension-sarcomere length relationships. The Lagrangian stress increased from a value of about 0.6 mN/mm2 at a sarcomere length of 2.35 microns to a value of about 7 mN/mm2 at a sarcomere length of 3.45 microns. These values of stress are about 8- to 30-fold less than those previously reported for intact frog atrial tissue and indicate that the resting tension of intact frog atrial preparations resides primarily in structures external to the individual cardiac cell.", "contents": "Sarcomere length-resting tension relation in single frog atrial cardiac cells. It generally has been thought that the relatively high resting tension characteristic of cardiac tissue resides in structures (collagen, elastin) external to the individual cardiac cells, but the evidence to support this conclusion has been indirect, since the resting tension of intact single cardiac cells has not been determined previously. The purpose of the present investigation was to determine the resting tension (stress)-sarcomere length relationships of single intact frog atrial cells. For tension determinations, a single cell was attached between two poly-L-lysine coated glass beams; one beam served as a compliant calibrated cantilevered force beam, and length changes were imposed on the cell by movement of the other beam. Coventional bright-field light microscope techniques were used to view the cell, the sarcomere pattern within the cell, and the position of the force beam. The resting tension of the intact cell increased from a value of about 10 nN at a sarcomere length of 2.35 microns to a value of about 130 nN at a sarcomere length of 3.45 microns. Lagrangian and Eulerian resting stress-sarcomere length relationships were computed from the resting tension-sarcomere length relationships. The Lagrangian stress increased from a value of about 0.6 mN/mm2 at a sarcomere length of 2.35 microns to a value of about 7 mN/mm2 at a sarcomere length of 3.45 microns. These values of stress are about 8- to 30-fold less than those previously reported for intact frog atrial tissue and indicate that the resting tension of intact frog atrial preparations resides primarily in structures external to the individual cardiac cell."}
{"id": "PMID:476874", "title": "Comparison of M-mode and cross-sectional echocardiography in infective endocarditis.", "content": "Cross-sectional and M-mode echocardiograms were performed on 23 consecutive patients with infective endocarditis. Both M-mode and cross-sectional echocardiography identified vegetations in 18 patients, 10 of whom required valve replacement within 1 month of presentation. Cross-sectional echocardiography alone identified a vegetation in one patient with a prosthetic valve. Neither technique identified vegetations in five instances. The size and shape of a vegetation on cross-sectional echocardiography did not accurately predict the need for early valve replacement of the incidence of major peripheral emboli.", "contents": "Comparison of M-mode and cross-sectional echocardiography in infective endocarditis. Cross-sectional and M-mode echocardiograms were performed on 23 consecutive patients with infective endocarditis. Both M-mode and cross-sectional echocardiography identified vegetations in 18 patients, 10 of whom required valve replacement within 1 month of presentation. Cross-sectional echocardiography alone identified a vegetation in one patient with a prosthetic valve. Neither technique identified vegetations in five instances. The size and shape of a vegetation on cross-sectional echocardiography did not accurately predict the need for early valve replacement of the incidence of major peripheral emboli."}
{"id": "PMID:476876", "title": "Can cardiac tamponade be diagnosed by echocardiography? Experimental studies.", "content": "The purpose of this study was to determine if respiratory variation and/or absolute size of echocardiographically measured right or left ventricular internal dimension at end-diastole (RVIDd or LIVIDd) are accurate indicators of the presence or severity of cardiac tamponade. We measured RVIDd or LVIDd by echocardiography in nine closed-chest, spontaneously breathing dogs in control and during hypotensive tamponade. With tamponade, the end-expiratory RVIDd and LVIDd were significantly smaller than control. Inspiratory increases in RVIDd and decreases in LVIDd were exaggerated during tamponade. Because of the wide range and overlap of RVIDd and LVIDd, no single expiratory value or amount of respiratory change indicated the presence or severity of tamponade. We conclude that if serial echocardiograms show a pericardial effusion, a decreasing end-expiratory RVIDd and LVIDd and an increasing percentage change in ventricular diameter with inspiration, a progressive degree of tamponade should be suspected However, a single echocardiogram cannot accurately predict the presence or severity of tamponade.", "contents": "Can cardiac tamponade be diagnosed by echocardiography? Experimental studies. The purpose of this study was to determine if respiratory variation and/or absolute size of echocardiographically measured right or left ventricular internal dimension at end-diastole (RVIDd or LIVIDd) are accurate indicators of the presence or severity of cardiac tamponade. We measured RVIDd or LVIDd by echocardiography in nine closed-chest, spontaneously breathing dogs in control and during hypotensive tamponade. With tamponade, the end-expiratory RVIDd and LVIDd were significantly smaller than control. Inspiratory increases in RVIDd and decreases in LVIDd were exaggerated during tamponade. Because of the wide range and overlap of RVIDd and LVIDd, no single expiratory value or amount of respiratory change indicated the presence or severity of tamponade. We conclude that if serial echocardiograms show a pericardial effusion, a decreasing end-expiratory RVIDd and LVIDd and an increasing percentage change in ventricular diameter with inspiration, a progressive degree of tamponade should be suspected However, a single echocardiogram cannot accurately predict the presence or severity of tamponade."}
{"id": "PMID:476877", "title": "Dynamic exercise echocardiography.", "content": "To evaluate left ventricular (LV) reserve, we developed a method of dynamic exercise echocardiography (DEE). Forty-six healthy persons and 47 cardiac patients performed bicycle ergometer exercise in the supine position. A special table was used on which the subjects could be firmly attached at shoulder level to prevent bodily movements which might disturb the recordings. In 83% of the subjects in whom a clear echocardiogram was obtained at rest, a clear echocardiogram was also obtained during dynamic exercise. During exercise, cardiac output estimated from the echocardiogram and that from the dye-dilution method showed an excellent correlation. The changes of the mean velocity of LV circumferential shortening during exercise permitted discrimination between older and younger healthy men, and also between healthy subjects and those with either mild or severe LV dysfunction. We conclude that DEE is useful for evaluating LV reserve.", "contents": "Dynamic exercise echocardiography. To evaluate left ventricular (LV) reserve, we developed a method of dynamic exercise echocardiography (DEE). Forty-six healthy persons and 47 cardiac patients performed bicycle ergometer exercise in the supine position. A special table was used on which the subjects could be firmly attached at shoulder level to prevent bodily movements which might disturb the recordings. In 83% of the subjects in whom a clear echocardiogram was obtained at rest, a clear echocardiogram was also obtained during dynamic exercise. During exercise, cardiac output estimated from the echocardiogram and that from the dye-dilution method showed an excellent correlation. The changes of the mean velocity of LV circumferential shortening during exercise permitted discrimination between older and younger healthy men, and also between healthy subjects and those with either mild or severe LV dysfunction. We conclude that DEE is useful for evaluating LV reserve."}
{"id": "PMID:476879", "title": "Assessment of left ventricular ejection fraction and volumes by real-time, two-dimensional echocardiography. A comparison of cineangiographic and radionuclide techniques.", "content": "Five different algorithms for determining left ventricular (LV) ejection fraction (EF) and volumes from two-dimensional echocardiographic examination (TDE) were compared with standard methods for obtaining EF and volume from x-ray cineangiography (cine) and EF from radionuclide ventriculography (RVG) in 35 patients. Although all methods correlated positively, the degree of correlation varied with the algorithm used. For EF determination, TDE algorithms (especially those using multiple planes of section) were superior to unidimensional algorithms commonly used with M-mode echocardiography. The best algorithm (modified Simpson's rule) correlated well enough with cine EF (r = 0.78; SEE 0.097) and RVG EF (r = 0.75; SEE 0.087) to make clinically useful estimates. TDE volumes also correlated meaningfully with cine end-diastolic and end-systole volumes (r = 084; n = 70) but were associated with a large standard error of the estimate (43 ml) and offered less advantage over unidimensional volume estimates. Quantitative application of TDE appears to be a useful noninvasive method of evaluating LVEF, but is not as useful for estimating LV volumes.", "contents": "Assessment of left ventricular ejection fraction and volumes by real-time, two-dimensional echocardiography. A comparison of cineangiographic and radionuclide techniques. Five different algorithms for determining left ventricular (LV) ejection fraction (EF) and volumes from two-dimensional echocardiographic examination (TDE) were compared with standard methods for obtaining EF and volume from x-ray cineangiography (cine) and EF from radionuclide ventriculography (RVG) in 35 patients. Although all methods correlated positively, the degree of correlation varied with the algorithm used. For EF determination, TDE algorithms (especially those using multiple planes of section) were superior to unidimensional algorithms commonly used with M-mode echocardiography. The best algorithm (modified Simpson's rule) correlated well enough with cine EF (r = 0.78; SEE 0.097) and RVG EF (r = 0.75; SEE 0.087) to make clinically useful estimates. TDE volumes also correlated meaningfully with cine end-diastolic and end-systole volumes (r = 084; n = 70) but were associated with a large standard error of the estimate (43 ml) and offered less advantage over unidimensional volume estimates. Quantitative application of TDE appears to be a useful noninvasive method of evaluating LVEF, but is not as useful for estimating LV volumes."}
{"id": "PMID:476882", "title": "Pathologic basis of thallium-201 scintigraphic defects in patients with fatal myocardial injury.", "content": "Using a quantitative, computer-aided circumferential profile technique, we have shown that thallium-201 scintigrams with large defects can identify a group of patients with a high mortality after acute myocardial infarction. To determine whether high-risk thallium scintigrams predict poor survival because of a critical loss of myocardium, we correlated infarct size in 24 autopsied patients with the extent of thallium defect in three views. Of 13 patients with large defects (computer score greater than or equal to 7.0) eight (62%) had greater than 25% loss of left ventricular (LV) myocardium, but five (38%) had smaller infarcts (4--24% of LV myocardium), suggesting that part of the scintigraphic defect was related to ischemia without necrosis. Eight of the nine patients with loss greater than or equal to 25% LV myocardium had large defects. In 10 of 11 patients with small defects (computer score less than 7.0), infarcts involved less than 20% of LV myocardium. Although scintigrams with large defects predicted a critical loss of myocardium in over 60% of our patients, they included an important second group, in which the scintigraphic defect appeared to reflect a small infarct and a large surrounding area of reversibly ischemic myocardium.", "contents": "Pathologic basis of thallium-201 scintigraphic defects in patients with fatal myocardial injury. Using a quantitative, computer-aided circumferential profile technique, we have shown that thallium-201 scintigrams with large defects can identify a group of patients with a high mortality after acute myocardial infarction. To determine whether high-risk thallium scintigrams predict poor survival because of a critical loss of myocardium, we correlated infarct size in 24 autopsied patients with the extent of thallium defect in three views. Of 13 patients with large defects (computer score greater than or equal to 7.0) eight (62%) had greater than 25% loss of left ventricular (LV) myocardium, but five (38%) had smaller infarcts (4--24% of LV myocardium), suggesting that part of the scintigraphic defect was related to ischemia without necrosis. Eight of the nine patients with loss greater than or equal to 25% LV myocardium had large defects. In 10 of 11 patients with small defects (computer score less than 7.0), infarcts involved less than 20% of LV myocardium. Although scintigrams with large defects predicted a critical loss of myocardium in over 60% of our patients, they included an important second group, in which the scintigraphic defect appeared to reflect a small infarct and a large surrounding area of reversibly ischemic myocardium."}
{"id": "PMID:476883", "title": "The effects of coronary artery disease on the ventricular fibrillation threshold in man.", "content": "The ventricular fibrillation threshold (VFT) was measured in 28 patients at the time of cardiac surgery. The VFT was measured with a 100 Hz train of 24 rectangular pulses positioned across the ST segment and T wave. Current was applied to the epicardial surface of either ventricle with a bipolar electrode probe. In six patients, the normal right VFT was 24.3 +/- 5.2 mA, and in 10 patients the normal left VFT was 33.6 +/- mA (p less than 0.05). In 12 patients with greater than or equal to 75% obstruction of the left anterior descending coronary artery, the left VFT was 18.6 +/- 6.9 mA. This value was significantly less than the left VFT in patients without coronary artery disease (p less than 0.001). This study shows that the VFT can be measured in man and that coronary artery disease reduces this parameter.", "contents": "The effects of coronary artery disease on the ventricular fibrillation threshold in man. The ventricular fibrillation threshold (VFT) was measured in 28 patients at the time of cardiac surgery. The VFT was measured with a 100 Hz train of 24 rectangular pulses positioned across the ST segment and T wave. Current was applied to the epicardial surface of either ventricle with a bipolar electrode probe. In six patients, the normal right VFT was 24.3 +/- 5.2 mA, and in 10 patients the normal left VFT was 33.6 +/- mA (p less than 0.05). In 12 patients with greater than or equal to 75% obstruction of the left anterior descending coronary artery, the left VFT was 18.6 +/- 6.9 mA. This value was significantly less than the left VFT in patients without coronary artery disease (p less than 0.001). This study shows that the VFT can be measured in man and that coronary artery disease reduces this parameter."}
{"id": "PMID:476884", "title": "Effect of submaximal exercise on vulnerability to fibrillation in the canine ventricle.", "content": "The risk of instantaneous death due to ventricular fibrillation was compared in resting and exercised dogs. Three weeks before testing, all dogs had bipolar left ventricular stimulating electrodes implanted and a reversible snare was placed around the anterior descending coronary artery. The dogs were randomly assigned to either an exercise (13 dogs) or a control (12 dogs) group. We measured ventricular fibrillation thresholds (VFTs) in all dogs before and after inducing ischemia by tightening the snare while the dogs stood at rest. The next day, nonischemic and ischemic VFTs were redetermined for control dogs at rest and for the exercise group during a treadmill run. No statistically significant changes were noted within and between groups in nonischemic or in ischemic VFTs at rest. In five exercise dogs, spontaneous ventricular fibrillation occurred during the first 8 minutes of the ischemic run, For the eight other exercise dogs, running increased the mean drop in VFTs during coronary occlusion by 23% (p less than 0.01). These data suggest that moderate dynamic exercise may greatly enhance the risk of ventricular fibrillation and sudden death in the presence of myocardial ischemia. In the absence of ischemia, exercise does not appear to increase vulnerability to ventricular fibrillation.", "contents": "Effect of submaximal exercise on vulnerability to fibrillation in the canine ventricle. The risk of instantaneous death due to ventricular fibrillation was compared in resting and exercised dogs. Three weeks before testing, all dogs had bipolar left ventricular stimulating electrodes implanted and a reversible snare was placed around the anterior descending coronary artery. The dogs were randomly assigned to either an exercise (13 dogs) or a control (12 dogs) group. We measured ventricular fibrillation thresholds (VFTs) in all dogs before and after inducing ischemia by tightening the snare while the dogs stood at rest. The next day, nonischemic and ischemic VFTs were redetermined for control dogs at rest and for the exercise group during a treadmill run. No statistically significant changes were noted within and between groups in nonischemic or in ischemic VFTs at rest. In five exercise dogs, spontaneous ventricular fibrillation occurred during the first 8 minutes of the ischemic run, For the eight other exercise dogs, running increased the mean drop in VFTs during coronary occlusion by 23% (p less than 0.01). These data suggest that moderate dynamic exercise may greatly enhance the risk of ventricular fibrillation and sudden death in the presence of myocardial ischemia. In the absence of ischemia, exercise does not appear to increase vulnerability to ventricular fibrillation."}
{"id": "PMID:476886", "title": "Evaluation of warning arrhythmias before paroxysmal ventricular tachycardia during acute myocardial infarction in man.", "content": "In order to determine the relationship of paroxysmal ventricular tachycardia (PVT) to any antecedent (premonitory) ventricular arrhythmias during the early phases of acute myocardial infarctions, 24-hour Holter monitoring was begun on 52 male patients an average of 12.6 hours after the onset of prolonged chest pain that was documented as acute infraction. Twenty-four patients had PVT and 28 did not. We analyzed in detail the incidence of frequency of premature ventricular complexes (PVCs), prematurity and pairing during the 10 minutes immediately preceding PVT from a continuous 10-minute rhythm strip. There was no positive correlation between PVT and the number or complexity of PVCs in the 10 minutes immediately before ventricular tachycardia. These findings suggest that there is no consistent pattern or frequency of ventricular arrhythmia that could be identified as premonitory for PVT during the immediate pre-PVT period, even during the acute phase of myocardial infarction in man.", "contents": "Evaluation of warning arrhythmias before paroxysmal ventricular tachycardia during acute myocardial infarction in man. In order to determine the relationship of paroxysmal ventricular tachycardia (PVT) to any antecedent (premonitory) ventricular arrhythmias during the early phases of acute myocardial infarctions, 24-hour Holter monitoring was begun on 52 male patients an average of 12.6 hours after the onset of prolonged chest pain that was documented as acute infraction. Twenty-four patients had PVT and 28 did not. We analyzed in detail the incidence of frequency of premature ventricular complexes (PVCs), prematurity and pairing during the 10 minutes immediately preceding PVT from a continuous 10-minute rhythm strip. There was no positive correlation between PVT and the number or complexity of PVCs in the 10 minutes immediately before ventricular tachycardia. These findings suggest that there is no consistent pattern or frequency of ventricular arrhythmia that could be identified as premonitory for PVT during the immediate pre-PVT period, even during the acute phase of myocardial infarction in man."}
{"id": "PMID:476887", "title": "Suppression of chronic ventricular arrhythmias with propranolol.", "content": "The antiarrhythmic efficacy of propranolol was evaluated in 32 patients with chronic high frequency ventricular arrhythmias in a placebo-controlled protocol. After a placebo control period, propranolol was begun and the dosage increased sequentially until arrhythmia suppression was achieved, side effects appeared, or a maximum dosage of 960 mg/day was reached. Computerized analysis of ambulatory recordings was used to quantify the arrhythmias. Twenty-four patients had 70--100% arrhythmia suppression at plasma levels ranging from 12--1100 ng/ml (end of dosing interval). Eight patients in this group had frequent episodes of ventricular tachycardia that were totally suppressed at or below the dosage that produced greater than or equal to 70% suppression of ventricular ectopic depolarizations (VEDs). A biphasic dose-response curve was seen in five patients who responded with a decrease in arrhythmia frequency in the lower ranges of dosages but had increased frequency of ectopic rhythms as the dosage was increased above the optimal level. Only one-third of patients responded at doses less than or equal to 160 mg/day. However, with dosages of 200--640 mg/day, an additional 40% responded. Propranolol appears to control ventricular arrhythmias safely and effectively in many patients. The finding that the antiarrhythmic effect in many patients required plasma concentrations greater than those that produce substantial beta-adrenergic blockage raises a question whether blockade of cardiac beta receptors can directly account for all of the antiarrhythmic actions of propranolol.", "contents": "Suppression of chronic ventricular arrhythmias with propranolol. The antiarrhythmic efficacy of propranolol was evaluated in 32 patients with chronic high frequency ventricular arrhythmias in a placebo-controlled protocol. After a placebo control period, propranolol was begun and the dosage increased sequentially until arrhythmia suppression was achieved, side effects appeared, or a maximum dosage of 960 mg/day was reached. Computerized analysis of ambulatory recordings was used to quantify the arrhythmias. Twenty-four patients had 70--100% arrhythmia suppression at plasma levels ranging from 12--1100 ng/ml (end of dosing interval). Eight patients in this group had frequent episodes of ventricular tachycardia that were totally suppressed at or below the dosage that produced greater than or equal to 70% suppression of ventricular ectopic depolarizations (VEDs). A biphasic dose-response curve was seen in five patients who responded with a decrease in arrhythmia frequency in the lower ranges of dosages but had increased frequency of ectopic rhythms as the dosage was increased above the optimal level. Only one-third of patients responded at doses less than or equal to 160 mg/day. However, with dosages of 200--640 mg/day, an additional 40% responded. Propranolol appears to control ventricular arrhythmias safely and effectively in many patients. The finding that the antiarrhythmic effect in many patients required plasma concentrations greater than those that produce substantial beta-adrenergic blockage raises a question whether blockade of cardiac beta receptors can directly account for all of the antiarrhythmic actions of propranolol."}
{"id": "PMID:476889", "title": "Characteristics of ventricular function in single ventricle.", "content": "Funtional characteristics of the single ventricle were studied by means of biplane angiocardiography in 34 patients. Group 1 consisted of 14 patients with normal or increased pulmonary vascular marking on chest film and no pulmonary stenosis. Group 2 included 20 patients with severe angiographic pulmonary stenosis and decreased pulmonary vascular marking. Ventricular volume parameters were calculated according to Simpson's rule and were compared with normal values. The sum of the normal left and right ventricular volumes was assumed to be 100%. In group 1, ventricular end-diastolic volumes averaged 143 +/- 11% and were significantly (p less than 0.001) larger than end-diastolic volumes in group 2 (81 +/- 4%). The presence or absence of severe pulmonary stenosis affecting pulmonary blood flow was a main factor regulating the ventricular chamber size in single ventricle. In both groups, the ejection fraction of a single ventricle was significantly lower than that of a normal left or right ventricle. Ventricular size and function in patients with a single ventricle should be carefully assessed before ventricular septation surgery.", "contents": "Characteristics of ventricular function in single ventricle. Funtional characteristics of the single ventricle were studied by means of biplane angiocardiography in 34 patients. Group 1 consisted of 14 patients with normal or increased pulmonary vascular marking on chest film and no pulmonary stenosis. Group 2 included 20 patients with severe angiographic pulmonary stenosis and decreased pulmonary vascular marking. Ventricular volume parameters were calculated according to Simpson's rule and were compared with normal values. The sum of the normal left and right ventricular volumes was assumed to be 100%. In group 1, ventricular end-diastolic volumes averaged 143 +/- 11% and were significantly (p less than 0.001) larger than end-diastolic volumes in group 2 (81 +/- 4%). The presence or absence of severe pulmonary stenosis affecting pulmonary blood flow was a main factor regulating the ventricular chamber size in single ventricle. In both groups, the ejection fraction of a single ventricle was significantly lower than that of a normal left or right ventricle. Ventricular size and function in patients with a single ventricle should be carefully assessed before ventricular septation surgery."}
{"id": "PMID:476893", "title": "Platelet suppressant therapy in patients with prosthetic cardiac valves. Relationship of clinical effectiveness to alteration of platelet survival time.", "content": "Platelet survival time (SURV) has correlated with thromboembolism in patients with prosthetic cardiac valves. Sulfinpyrazone increases SURV. SURV (autologous labeling with 51Chromium) was measured in 126 patients who had aortic or mitral valve replacement. These patients were followed prospectively. Ninety-four with shortened SURV received sulfinpyrazone; 32 with normal SURV were not treated with platelet suppressants. Eighty-seven patients were anticoagulated with warfarin--67 with shortened SURV and 20 with normal SURV. Eleven patients have had thromboembolism, and all had shortened SURV (2.4 +/- 0.08 days; average half-time +/- SEM; normal 3.7 +/- 0.03 days; n = 26) none had an increase of SURV with sulfinpyrazone (2.3 +/- 0.09 days). Of 83 patients with shortened SURV who did not have embolism, sulfinpyrazone increased SURV in 59 (71%) 2.6 +/- 0.05 to 2.9 +/- 0.06 days). Of 35 patients with shortened SURV who failed to increase SURV with sulfinpyrazone, 11 (31%) had embolism; none of 59 (0%) with an increase of SURV with sulfinpyrazone had thromboembolism. These results suggest that patients with thromboembolism after prosthetic cardiac valve replacement have shortened SURV and that patients treated with slufinpyrazone who have thromboembolism do not have an increased SURV.", "contents": "Platelet suppressant therapy in patients with prosthetic cardiac valves. Relationship of clinical effectiveness to alteration of platelet survival time. Platelet survival time (SURV) has correlated with thromboembolism in patients with prosthetic cardiac valves. Sulfinpyrazone increases SURV. SURV (autologous labeling with 51Chromium) was measured in 126 patients who had aortic or mitral valve replacement. These patients were followed prospectively. Ninety-four with shortened SURV received sulfinpyrazone; 32 with normal SURV were not treated with platelet suppressants. Eighty-seven patients were anticoagulated with warfarin--67 with shortened SURV and 20 with normal SURV. Eleven patients have had thromboembolism, and all had shortened SURV (2.4 +/- 0.08 days; average half-time +/- SEM; normal 3.7 +/- 0.03 days; n = 26) none had an increase of SURV with sulfinpyrazone (2.3 +/- 0.09 days). Of 83 patients with shortened SURV who did not have embolism, sulfinpyrazone increased SURV in 59 (71%) 2.6 +/- 0.05 to 2.9 +/- 0.06 days). Of 35 patients with shortened SURV who failed to increase SURV with sulfinpyrazone, 11 (31%) had embolism; none of 59 (0%) with an increase of SURV with sulfinpyrazone had thromboembolism. These results suggest that patients with thromboembolism after prosthetic cardiac valve replacement have shortened SURV and that patients treated with slufinpyrazone who have thromboembolism do not have an increased SURV."}
{"id": "PMID:476895", "title": "Evaluation of the echocardiogram as an epidemiologic tool in an asymptomatic population.", "content": "An asymptomatic adult population of 196 men and women was studied with the echocardiogram to derive age- and sex-specific \"normal\" values for a number of clinically used echocardiograhic variables. The results are in general agreement with previously published normal values. Body position during the examination, age and sex influence the echocardiographic results; body surface area correction normalized most of these effects. The prevalence of occult abnormalities determined by the echocardiogram is 7%; the most common finding was mitral valve prolapse. Inter- and intraobserver variability was assessed. The interobserver differences found on analysis are statistically, but not clinically , significant. The echocardiogram appears to be a suitable tool to use in epidemiologic studies to detect selected cardiac abnormalities, but is limited for this purpose because some subjects in such a population cannot be adequately examined.", "contents": "Evaluation of the echocardiogram as an epidemiologic tool in an asymptomatic population. An asymptomatic adult population of 196 men and women was studied with the echocardiogram to derive age- and sex-specific \"normal\" values for a number of clinically used echocardiograhic variables. The results are in general agreement with previously published normal values. Body position during the examination, age and sex influence the echocardiographic results; body surface area correction normalized most of these effects. The prevalence of occult abnormalities determined by the echocardiogram is 7%; the most common finding was mitral valve prolapse. Inter- and intraobserver variability was assessed. The interobserver differences found on analysis are statistically, but not clinically , significant. The echocardiogram appears to be a suitable tool to use in epidemiologic studies to detect selected cardiac abnormalities, but is limited for this purpose because some subjects in such a population cannot be adequately examined."}
{"id": "PMID:476896", "title": "Electrophysiologic studies in patients with ventricular inversion and \"corrected transposition\".", "content": "We evaluated the intracardiac conduction intervals using His bundle recordings in 40 patients with ventricular inversion and 1-transposition of the great arteries. Twenty-nine subjects had 1:1 atrioventricular (AV) conduction. In 15 of those with normal PR intervals and QRS durations, the conduction intervals were not different from those of subjects with normal hearts. In the 14 patients with first-degree AV block, the block was located between the sinus node and AV node in four, between the low right atrium and bundle of His in seven, and below the common bundle of His in four. In 11 subjects with complete AV block, the stie of block was above the site of the His potential in four, below in two and within the His bundle in one. In four patients we could not record a His potential and thus could not localize the site of block. Complete block below the His recording site was associated with syncope in one patient and sudden death in another. His bundle recording is a safe technique for studying the conduction system in children with ventricular inversion and 1-transposition of the great arteries.", "contents": "Electrophysiologic studies in patients with ventricular inversion and \"corrected transposition\". We evaluated the intracardiac conduction intervals using His bundle recordings in 40 patients with ventricular inversion and 1-transposition of the great arteries. Twenty-nine subjects had 1:1 atrioventricular (AV) conduction. In 15 of those with normal PR intervals and QRS durations, the conduction intervals were not different from those of subjects with normal hearts. In the 14 patients with first-degree AV block, the block was located between the sinus node and AV node in four, between the low right atrium and bundle of His in seven, and below the common bundle of His in four. In 11 subjects with complete AV block, the stie of block was above the site of the His potential in four, below in two and within the His bundle in one. In four patients we could not record a His potential and thus could not localize the site of block. Complete block below the His recording site was associated with syncope in one patient and sudden death in another. His bundle recording is a safe technique for studying the conduction system in children with ventricular inversion and 1-transposition of the great arteries."}
{"id": "PMID:476897", "title": "Depressed responsiveness to vasoconstrictor and dilator agents and baroreflex sensitivity in conscious, newborn lambs.", "content": "The effects of vasoconstrictors and vasodilators were compared in conscious, newborn lambs and adult sheep instrumented with electromagnetic flow probes on the ascending aorta and catheters in the thoracic aorta. Methoxamine, angiotensin II, norepinephrine, nitroglycerin and isoproterenol were administered intravenously to evaluate their effects on arterial pressure, cardiac output and systemic vascular resistance (SVR). The difference in response between adults and newborns was most apparent with methoxamine. Methoxamine, 400 micrograms/kg, i.v., which increased mean arterial pressure by 57 +/- 6% and SVR by 278 +/- 27% in newborn lambs, caused greater increases (p less than 0.01) of 81 +/- 8% and 1418 +/- 141%, respectively, in the adults. Responses also differed significantly between newborn and adult animals to norepinephrine, angiotensin II, nitroglycerin and isoproterenol. In a second group of animals in which smaller amounts of methoxamine and isoproterenol were injected directly into the terminal aorta, changes in terminal aortic flow and resistance were examined. Again, both vasoconstrictor and vasodilator responses were more marked in adults than in newborns. Finally, the sensitivity of the arterial baroreceptor reflex was evaluated by comparing the regression of pulse interval (PI) on systolic arterial pressure (SAP) after an intravenous dose of methoxamine in conscious, adult and newborn animals. The PI/SAP slopes in adult sheep, 45.4 +/- 3.5 msec/mm Hg, were significantly greater (p less than 0.01) than in newborn lambs, 11.7 +/- 2.2 msec/mm Hg.", "contents": "Depressed responsiveness to vasoconstrictor and dilator agents and baroreflex sensitivity in conscious, newborn lambs. The effects of vasoconstrictors and vasodilators were compared in conscious, newborn lambs and adult sheep instrumented with electromagnetic flow probes on the ascending aorta and catheters in the thoracic aorta. Methoxamine, angiotensin II, norepinephrine, nitroglycerin and isoproterenol were administered intravenously to evaluate their effects on arterial pressure, cardiac output and systemic vascular resistance (SVR). The difference in response between adults and newborns was most apparent with methoxamine. Methoxamine, 400 micrograms/kg, i.v., which increased mean arterial pressure by 57 +/- 6% and SVR by 278 +/- 27% in newborn lambs, caused greater increases (p less than 0.01) of 81 +/- 8% and 1418 +/- 141%, respectively, in the adults. Responses also differed significantly between newborn and adult animals to norepinephrine, angiotensin II, nitroglycerin and isoproterenol. In a second group of animals in which smaller amounts of methoxamine and isoproterenol were injected directly into the terminal aorta, changes in terminal aortic flow and resistance were examined. Again, both vasoconstrictor and vasodilator responses were more marked in adults than in newborns. Finally, the sensitivity of the arterial baroreceptor reflex was evaluated by comparing the regression of pulse interval (PI) on systolic arterial pressure (SAP) after an intravenous dose of methoxamine in conscious, adult and newborn animals. The PI/SAP slopes in adult sheep, 45.4 +/- 3.5 msec/mm Hg, were significantly greater (p less than 0.01) than in newborn lambs, 11.7 +/- 2.2 msec/mm Hg."}
{"id": "PMID:476898", "title": "Determination of His-Purkinje refractoriness in man with His bundle pacing.", "content": "Consistent His bundle pacing was accomplished in one of 60 consecutive patients. Intracardiac electrophysiologic studies in this patient revealed rapid atrioventricular nodal conduction (AH = 55 msec). While the relative refractory period of the His-Purkinje system as a whole determined by His bundle pacing (using the extrastimulus technique) was identical to that determined by atrial pacing, changes in right bundle branch refractoriness differed. An atrial extrasystolic interval of 370 msec resulted in right bundle branch block, whereas direct His pacing at a shorter extrasystolic interval (360 msec) failed to produce a bundle branch block pattern. The effective refractory period of the His-Purkinje system could not be determined by atrial pacing because of atrial refractoriness, but was obtained by His bundle pacing. Theoretically, direct His bundle pacing may be of value in determining His-Purkinje system refractoriness when this parameter is unobtainable due to either atrial or atrioventricular nodal refractoriness. This technique appears to have limited clinical usefulness, however, because of the very small success rates. Finally, His-Purkinje system refractoriness may differ, depending on site of stimulation, presumably due to differing inputs into the His-Purkinje system.", "contents": "Determination of His-Purkinje refractoriness in man with His bundle pacing. Consistent His bundle pacing was accomplished in one of 60 consecutive patients. Intracardiac electrophysiologic studies in this patient revealed rapid atrioventricular nodal conduction (AH = 55 msec). While the relative refractory period of the His-Purkinje system as a whole determined by His bundle pacing (using the extrastimulus technique) was identical to that determined by atrial pacing, changes in right bundle branch refractoriness differed. An atrial extrasystolic interval of 370 msec resulted in right bundle branch block, whereas direct His pacing at a shorter extrasystolic interval (360 msec) failed to produce a bundle branch block pattern. The effective refractory period of the His-Purkinje system could not be determined by atrial pacing because of atrial refractoriness, but was obtained by His bundle pacing. Theoretically, direct His bundle pacing may be of value in determining His-Purkinje system refractoriness when this parameter is unobtainable due to either atrial or atrioventricular nodal refractoriness. This technique appears to have limited clinical usefulness, however, because of the very small success rates. Finally, His-Purkinje system refractoriness may differ, depending on site of stimulation, presumably due to differing inputs into the His-Purkinje system."}
{"id": "PMID:476905", "title": "Prevalence of asthma: a comparative study of urban and rural Xhosa children.", "content": "An epidemiological study was undertaken to determine the prevalence of asthma in young urban and rural black (Xhosa) children. One thousand three hundred and seventy five children were studied, 694 from a Cape Town african township and 671 from a rural area in Transkei. The exercise tolerance test which required free range running at maximum effort for 6 min was used to identify asthmatic subjects. A fall of 15% or more in the post-exercise FEV1 and PEFR values was regarded as a positive result. Twenty-three children were found to be asthmatic, twenty-two from the city area, but only one from the country, giving a prevalence figure for asthma of 3.17% in the first group and 0.14% for the second. Possible reasons for these differences are discussed. The exercise tolerance test was found to be a useful tool for epidemiological studies of asthma.", "contents": "Prevalence of asthma: a comparative study of urban and rural Xhosa children. An epidemiological study was undertaken to determine the prevalence of asthma in young urban and rural black (Xhosa) children. One thousand three hundred and seventy five children were studied, 694 from a Cape Town african township and 671 from a rural area in Transkei. The exercise tolerance test which required free range running at maximum effort for 6 min was used to identify asthmatic subjects. A fall of 15% or more in the post-exercise FEV1 and PEFR values was regarded as a positive result. Twenty-three children were found to be asthmatic, twenty-two from the city area, but only one from the country, giving a prevalence figure for asthma of 3.17% in the first group and 0.14% for the second. Possible reasons for these differences are discussed. The exercise tolerance test was found to be a useful tool for epidemiological studies of asthma."}
{"id": "PMID:476907", "title": "Asthma, IgE and environment in northern Nigeria.", "content": "Sixty-four asthmatics and seventy-five control subjects from rural villages and various urban settings have been investigated. Rural asthmatics had a significantly higher IgE level than both urban asthmatics and village controls. Urban asthmatics had similar IgE levels to urban controls. There was no relationship between IgE levels and the results of skin tests for asthma allergens. Stool parasites were found as frequently in asthmatics as in controls. Our findings do not support the theory that parasitic infestation, high IgE levels or rural living protect from asthma.", "contents": "Asthma, IgE and environment in northern Nigeria. Sixty-four asthmatics and seventy-five control subjects from rural villages and various urban settings have been investigated. Rural asthmatics had a significantly higher IgE level than both urban asthmatics and village controls. Urban asthmatics had similar IgE levels to urban controls. There was no relationship between IgE levels and the results of skin tests for asthma allergens. Stool parasites were found as frequently in asthmatics as in controls. Our findings do not support the theory that parasitic infestation, high IgE levels or rural living protect from asthma."}
{"id": "PMID:476906", "title": "Bronchial asthma in the southern Maldives.", "content": "A whole population survey was performed in Addu Atoll, southern Maldives to establish the prevalence of bronchial asthma. Assessments of atopic eczema, allergic rhinitis, conjunctivitis and intestinal infection were made, and serum specific IgE titres to a number of allergens were measured. Asthma is a common condition in the southern Maldives, and there is no evidence that the IgE produced by intestinal helminths prevents the condition.", "contents": "Bronchial asthma in the southern Maldives. A whole population survey was performed in Addu Atoll, southern Maldives to establish the prevalence of bronchial asthma. Assessments of atopic eczema, allergic rhinitis, conjunctivitis and intestinal infection were made, and serum specific IgE titres to a number of allergens were measured. Asthma is a common condition in the southern Maldives, and there is no evidence that the IgE produced by intestinal helminths prevents the condition."}
{"id": "PMID:476908", "title": "Serum IgE in healthy infants fed breast milk or cow's milk-based formulas.", "content": "A group of ninety-five infants was followed from birth to one year of age, and the total serum IgE concentration was studied. The infants were fed on breast milk, home-prepared cow's milk formula, or proprietary infant milk formula. Solid foods were introduced at 3.5 months of age. The breast milk group had lower IgE than the cow's milk group up to 4 months of age, i.e. until the end of exclusive milk feeding. The formula group had intermediate IgE values. The difference was not due to the presence of IgE antibodies to cow's milk in bottle-fed infants, since no such antibodies could be detected. After the introduction of solid foods the differences between the groups disappeared. Our data suggest that exclusive breast milk feeding can reduce total serum IgE concentration in early infancy. Reference values for unselected infants and for infants with no atopic manifestations are reported.", "contents": "Serum IgE in healthy infants fed breast milk or cow's milk-based formulas. A group of ninety-five infants was followed from birth to one year of age, and the total serum IgE concentration was studied. The infants were fed on breast milk, home-prepared cow's milk formula, or proprietary infant milk formula. Solid foods were introduced at 3.5 months of age. The breast milk group had lower IgE than the cow's milk group up to 4 months of age, i.e. until the end of exclusive milk feeding. The formula group had intermediate IgE values. The difference was not due to the presence of IgE antibodies to cow's milk in bottle-fed infants, since no such antibodies could be detected. After the introduction of solid foods the differences between the groups disappeared. Our data suggest that exclusive breast milk feeding can reduce total serum IgE concentration in early infancy. Reference values for unselected infants and for infants with no atopic manifestations are reported."}
{"id": "PMID:476909", "title": "Natural history of allergy to Hymenoptera.", "content": "One hundred and nineteen patients who had a generalized reaction to a bee sting and who subsequently had a history of being re-stung before desensitization were studied. The reaction, in these 119 patients was as follows: 44.5% had an improved grade of reaction, 42.9% had the same grade of reaction and 12.6% had a worse grade of reaction. Of these 119 patients, 39.5% had only a local reaction to their re-sting. Those patients who were re-stung within 2 weeks (anergic period) or over 5 years after a generalized reaction to a sting had significantly improved response. Results of pretreatment RAST to venoms of hornet, yellow jacket, wasp, honey bee and to phospholipase A appeared to correlate with the clinical signs and symptoms of the previous sting in 75% of cases.", "contents": "Natural history of allergy to Hymenoptera. One hundred and nineteen patients who had a generalized reaction to a bee sting and who subsequently had a history of being re-stung before desensitization were studied. The reaction, in these 119 patients was as follows: 44.5% had an improved grade of reaction, 42.9% had the same grade of reaction and 12.6% had a worse grade of reaction. Of these 119 patients, 39.5% had only a local reaction to their re-sting. Those patients who were re-stung within 2 weeks (anergic period) or over 5 years after a generalized reaction to a sting had significantly improved response. Results of pretreatment RAST to venoms of hornet, yellow jacket, wasp, honey bee and to phospholipase A appeared to correlate with the clinical signs and symptoms of the previous sting in 75% of cases."}
{"id": "PMID:476910", "title": "Haemagglutinating antibodies to cat dander in relation to exposure and respiratory allergy.", "content": "Cat epithelial extract was coupled with chromic chloride to red cells and used as an antigen in a haemagglutination assay. Agglutinins coexisted with IgE type antibodies in 44% of patients with respiratory allergy and sensitivity to cat dander but occurred in only 1% of asthmatic patients sensitized to other allergens or in controls. The agglutinins were detected in six out of 104 cat fanciers, and three of those with agglutinins had asthma. The agglutinins were of IgG type and were heterogeneous in specificity.", "contents": "Haemagglutinating antibodies to cat dander in relation to exposure and respiratory allergy. Cat epithelial extract was coupled with chromic chloride to red cells and used as an antigen in a haemagglutination assay. Agglutinins coexisted with IgE type antibodies in 44% of patients with respiratory allergy and sensitivity to cat dander but occurred in only 1% of asthmatic patients sensitized to other allergens or in controls. The agglutinins were detected in six out of 104 cat fanciers, and three of those with agglutinins had asthma. The agglutinins were of IgG type and were heterogeneous in specificity."}
{"id": "PMID:476911", "title": "Treatment of superficial cancers by combination hyperthermia and radiation therapy.", "content": "Clinical studies are being carried out to evaluate whether radiation combined with hyperthermia evokes better tumor control than that achieved with radiation alone. Thus far, 54 patients with various superficial cancers have been treated with combination hyperthermia and radiation. The heating methods used have been temperature regulated water bath immersion and radiofrequency inductive heating. Hyperthermia alone has caused significant, though transitory, tumor regression in 10 out of 19 patients. Selective heating of tumor tissues has been achieved in 20 out of 24 lesions following radiofrequency inductive hyperthermia. The overall local tumor control rate after the combined therapy was 78 percent compared to 26 percent after radiation alone.", "contents": "Treatment of superficial cancers by combination hyperthermia and radiation therapy. Clinical studies are being carried out to evaluate whether radiation combined with hyperthermia evokes better tumor control than that achieved with radiation alone. Thus far, 54 patients with various superficial cancers have been treated with combination hyperthermia and radiation. The heating methods used have been temperature regulated water bath immersion and radiofrequency inductive heating. Hyperthermia alone has caused significant, though transitory, tumor regression in 10 out of 19 patients. Selective heating of tumor tissues has been achieved in 20 out of 24 lesions following radiofrequency inductive hyperthermia. The overall local tumor control rate after the combined therapy was 78 percent compared to 26 percent after radiation alone."}
{"id": "PMID:476915", "title": "A model program for education and training of clinical chemists.", "content": "Some general guidelines can help in the assessment of clinical chemistry training and training programs. A clinical chemist must be, first of all, a good chemist, but to this must be added other knowledge and skills. A program that begins with a bachelor's degree in chemistry equivalent to the American Chemical Society's standards is proposed for the clinical chemistry technologist. The model provides more flexibility as the education proceeds toward the doctoral and postdoctoral levels and as the opportunities and responsibilities become increasingly more diverse. An essential portion of the training at all levels is experience in a clinical laboratory. It is strongly recommended that this be started early and be continued throughout the program. Certification by an independent agency (National Registry in Clinical Chemistry or American Board of Clinical Chemistry) is strongly recommended as a final step in all plans.", "contents": "A model program for education and training of clinical chemists. Some general guidelines can help in the assessment of clinical chemistry training and training programs. A clinical chemist must be, first of all, a good chemist, but to this must be added other knowledge and skills. A program that begins with a bachelor's degree in chemistry equivalent to the American Chemical Society's standards is proposed for the clinical chemistry technologist. The model provides more flexibility as the education proceeds toward the doctoral and postdoctoral levels and as the opportunities and responsibilities become increasingly more diverse. An essential portion of the training at all levels is experience in a clinical laboratory. It is strongly recommended that this be started early and be continued throughout the program. Certification by an independent agency (National Registry in Clinical Chemistry or American Board of Clinical Chemistry) is strongly recommended as a final step in all plans."}
{"id": "PMID:476917", "title": "Hemoglobinuria and hematuria: accuracy and precision of laboratory diagnosis.", "content": "Five methods of assessing blood in urine were studied and compared: sedimentation count, hemacytometry, \"Clini-lab\" reagent strip and \"N-Multistix\" and \"ChemStrip-8\" dipsticks. The minimum sensitivity of two commercially available urine dip-stick procedures for blood was established. The study includes method association, assay variation among and within technologists, accuracy, precision, and sensitivity. Our results demonstrate generally poor association between results by the dip-stick procedures and the hemacytometer or sedimentation count. Results by the last two procedures also showed very poor association with each other.", "contents": "Hemoglobinuria and hematuria: accuracy and precision of laboratory diagnosis. Five methods of assessing blood in urine were studied and compared: sedimentation count, hemacytometry, \"Clini-lab\" reagent strip and \"N-Multistix\" and \"ChemStrip-8\" dipsticks. The minimum sensitivity of two commercially available urine dip-stick procedures for blood was established. The study includes method association, assay variation among and within technologists, accuracy, precision, and sensitivity. Our results demonstrate generally poor association between results by the dip-stick procedures and the hemacytometer or sedimentation count. Results by the last two procedures also showed very poor association with each other."}
{"id": "PMID:476916", "title": "Column chromatography and immunoassay compared for measuring the isoenzymes of aspartate aminotransferase in serum.", "content": "We compare a column-chromatographic method and a homogeneous immunoassay method for separately measuring the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase. Analytical recovery for the two methods averaged 102% (SD, 2%) and 103% (SD, 4%), respectively, for 11 pools prepared by adding the purified isoenzymes to serum and 102% (SD 8.9%) and 89% (SD, 8.1%) for 26 unaltered specimens of human serum. In comparing the results of the immunoassay method (y) to the chromatographic method (x), our measurements agreed closely for the mitochondrial (y = 0.947 X + 7, r = 0.9991, standard error of estimate = 2.9 U/L) and cytoplasmic (y = 0.92x-6, r = 0.9995, standard error of estimate = 2.1 U/L) isoenzymes in pools prepared from the purified isoenzymes. Similar measurements of the 26 human serum specimens yielded the following results for least-squares evaluation; cytoplasmic isoenzyme y = 1.03x-11, r = 0.994, and standard error of estimate = 6.0 U/L; mitochondrial isoenzyme y = 0.75x+0, r = 0.927, and standard error of estimate = 3.9 U/L.", "contents": "Column chromatography and immunoassay compared for measuring the isoenzymes of aspartate aminotransferase in serum. We compare a column-chromatographic method and a homogeneous immunoassay method for separately measuring the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase. Analytical recovery for the two methods averaged 102% (SD, 2%) and 103% (SD, 4%), respectively, for 11 pools prepared by adding the purified isoenzymes to serum and 102% (SD 8.9%) and 89% (SD, 8.1%) for 26 unaltered specimens of human serum. In comparing the results of the immunoassay method (y) to the chromatographic method (x), our measurements agreed closely for the mitochondrial (y = 0.947 X + 7, r = 0.9991, standard error of estimate = 2.9 U/L) and cytoplasmic (y = 0.92x-6, r = 0.9995, standard error of estimate = 2.1 U/L) isoenzymes in pools prepared from the purified isoenzymes. Similar measurements of the 26 human serum specimens yielded the following results for least-squares evaluation; cytoplasmic isoenzyme y = 1.03x-11, r = 0.994, and standard error of estimate = 6.0 U/L; mitochondrial isoenzyme y = 0.75x+0, r = 0.927, and standard error of estimate = 3.9 U/L."}
{"id": "PMID:476918", "title": "Improved radioimmunoassay for 11-deoxycortisol (compound S) in plasma.", "content": "We describe a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9, and 69.9 micrograms/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 micrograms/L gave inter-assay CV's of 10.1, 9.7, and 6.2%, respectively. A normal range (mean +/- 2SD) of 0.5 to 12.0 micrograms/L was obtained. This procedure is useful for the specific determination of 11-deoxycortisol, commonly determined in blood as part of the metyrapone test for assessing pituitary corticotropin reserve.", "contents": "Improved radioimmunoassay for 11-deoxycortisol (compound S) in plasma. We describe a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9, and 69.9 micrograms/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 micrograms/L gave inter-assay CV's of 10.1, 9.7, and 6.2%, respectively. A normal range (mean +/- 2SD) of 0.5 to 12.0 micrograms/L was obtained. This procedure is useful for the specific determination of 11-deoxycortisol, commonly determined in blood as part of the metyrapone test for assessing pituitary corticotropin reserve."}
{"id": "PMID:476919", "title": "Determination of 4-O-methylated catecholamine metabolites in urine by mass fragmentography.", "content": "We describe the determination of the 4-O-methylated catecholamine metabolites 4-methoxy-3-hydroxyphenylacetic acid (iso-HVA), 4-methoxy-3-hydroxyphenylmandelic acid (iso-VMA) and 4-methoxy-3-hydroxyphenylethylene glycol (iso-MOPEG) in urine with use of mass fragmentography and deuterated internal standards. Normal values, expressed in terms of creatinine, are given as a function of age. Urinary excretion values during neuroblastoma, pheochromocytoma, and parkinsonism treated with L-DOPA, and during the intravenous administration of dopamine are discussed. For normal persons, the mean percentages (+/- SD) of 4-O-methylated metabolites relative to their 3-O-methylated analogs were 6.29 +/- 2.79% (iso-HVA/HVA), 0.70 +/- 0.57% (iso-VMA/VMA), and 0.77 +/- 0.53% (iso-MOPEG/MOPEG). We observed no significant changes in these parameters for the patients studied and conclude that the determination of 4-O-methylated catecholamine metabolites for these patients does not add information to the results obtained from their quantitatively more important 3-O-methylated analogs.", "contents": "Determination of 4-O-methylated catecholamine metabolites in urine by mass fragmentography. We describe the determination of the 4-O-methylated catecholamine metabolites 4-methoxy-3-hydroxyphenylacetic acid (iso-HVA), 4-methoxy-3-hydroxyphenylmandelic acid (iso-VMA) and 4-methoxy-3-hydroxyphenylethylene glycol (iso-MOPEG) in urine with use of mass fragmentography and deuterated internal standards. Normal values, expressed in terms of creatinine, are given as a function of age. Urinary excretion values during neuroblastoma, pheochromocytoma, and parkinsonism treated with L-DOPA, and during the intravenous administration of dopamine are discussed. For normal persons, the mean percentages (+/- SD) of 4-O-methylated metabolites relative to their 3-O-methylated analogs were 6.29 +/- 2.79% (iso-HVA/HVA), 0.70 +/- 0.57% (iso-VMA/VMA), and 0.77 +/- 0.53% (iso-MOPEG/MOPEG). We observed no significant changes in these parameters for the patients studied and conclude that the determination of 4-O-methylated catecholamine metabolites for these patients does not add information to the results obtained from their quantitatively more important 3-O-methylated analogs."}
{"id": "PMID:476920", "title": "Arylesterase in serum: elaboration and clinical application of a fixed-incubation method.", "content": "A sensitive, specific, and simple method for determining serum or urine arylesterase (EC 3.1.1.2) is described. The enzyme acts on phenyl acetate to release phenol, which produces a stable indophenol dye with 4-aminoantipyrine and potassium ferricyanide. Arylesterase, a thiol enzyme, is reactivated by 2-mercaptoethanol and by cysteine, but not by reduced glutathione. Calcium is indispensable to stabilize and to activate (Km = 0.85 mmol/L) the enzyme; complete protection is achieved at CaCl2 20 mmol/L. Magnesium acts as a weak (Ki = 116 mmol/L), lanthanum as a potent (Ki = 5 mumol/L) competitive inhibitor. The activity is measured in diluted sera at phenyl acetate 4.0 mmol/L (Km = 1.12 mmol/L), pH 7.8 and 25 degrees C. The normal range extends from 53 to 186 kU/L, and four isoenzymes are present in sera from healthy adults. Arylesterase decreases in hepatic disorders, especially in cirrhosis and carcinoma of the liver, with reduction of the penultimate fraction in polyacryalmide gel electrophoresis.", "contents": "Arylesterase in serum: elaboration and clinical application of a fixed-incubation method. A sensitive, specific, and simple method for determining serum or urine arylesterase (EC 3.1.1.2) is described. The enzyme acts on phenyl acetate to release phenol, which produces a stable indophenol dye with 4-aminoantipyrine and potassium ferricyanide. Arylesterase, a thiol enzyme, is reactivated by 2-mercaptoethanol and by cysteine, but not by reduced glutathione. Calcium is indispensable to stabilize and to activate (Km = 0.85 mmol/L) the enzyme; complete protection is achieved at CaCl2 20 mmol/L. Magnesium acts as a weak (Ki = 116 mmol/L), lanthanum as a potent (Ki = 5 mumol/L) competitive inhibitor. The activity is measured in diluted sera at phenyl acetate 4.0 mmol/L (Km = 1.12 mmol/L), pH 7.8 and 25 degrees C. The normal range extends from 53 to 186 kU/L, and four isoenzymes are present in sera from healthy adults. Arylesterase decreases in hepatic disorders, especially in cirrhosis and carcinoma of the liver, with reduction of the penultimate fraction in polyacryalmide gel electrophoresis."}
{"id": "PMID:476921", "title": "Chemical inhibition used in a kinetic urease/glutamate dehydrogenase method for urea in serum.", "content": "We describe a fixed-time-interval, kinetic inhibition method, with use of a competitive inhibitor (l) of the urease/glutamate dehydrogenase reaction to increase the \"apparent\" Michaelis constant by a factor of (1 + [l]lKl). This allows greater flexibility in selecting an appropriate sample dilution for kinetic determinations of urea in serum (i.e., [S]lKm ratio). Nine compounds were screened as potential inhibitors for this study. Adding 5 mmol of hydroxyurea per liter increases the \"apparent\" Michaelis constant for the coupled enzyme reaction by 10-fold. We used a sample dilution of 21-fold vs. dilutions of 141- to 350-fold for previously reported kinetic methods. Mean analytical recovery with this method was 100.2%. Reaction rate vs. urea concentration was linear, and complete recovery extended to 30 mmol of urea per liter. Of 22 potential interferents, only fluoride (250 mmol/L) and bilirubin (1 mmol/L, or 580 mg/L) caused greater than 5% interference. We discuss precision and effects of specimen dilution, and compare results for 100 specimens with those by a manual Berthelot-indophenol method, a manual diacetyl monoxime method, and a diacetyl monoxime method adapted to continuous-flow analysis.", "contents": "Chemical inhibition used in a kinetic urease/glutamate dehydrogenase method for urea in serum. We describe a fixed-time-interval, kinetic inhibition method, with use of a competitive inhibitor (l) of the urease/glutamate dehydrogenase reaction to increase the \"apparent\" Michaelis constant by a factor of (1 + [l]lKl). This allows greater flexibility in selecting an appropriate sample dilution for kinetic determinations of urea in serum (i.e., [S]lKm ratio). Nine compounds were screened as potential inhibitors for this study. Adding 5 mmol of hydroxyurea per liter increases the \"apparent\" Michaelis constant for the coupled enzyme reaction by 10-fold. We used a sample dilution of 21-fold vs. dilutions of 141- to 350-fold for previously reported kinetic methods. Mean analytical recovery with this method was 100.2%. Reaction rate vs. urea concentration was linear, and complete recovery extended to 30 mmol of urea per liter. Of 22 potential interferents, only fluoride (250 mmol/L) and bilirubin (1 mmol/L, or 580 mg/L) caused greater than 5% interference. We discuss precision and effects of specimen dilution, and compare results for 100 specimens with those by a manual Berthelot-indophenol method, a manual diacetyl monoxime method, and a diacetyl monoxime method adapted to continuous-flow analysis."}
{"id": "PMID:476922", "title": "DuPont aca III performance as tested according to NCCLS guidelines.", "content": "We evaluated the analytical performance of 10 representative channels on the new microprocessor-controlled DuPont aca III according to the guidelines (draft documents PSEP-2, 3, and 4) proposed by the Instrument Evaluation Subcommittee of the National Committee for Clinical Laboratory Standards. These guidelines were used for the experimental design and data analysis for the precision and accuracy testing, the latter by comparison with an aca II, with results of atomic absorption spectroscopy for calcium, and with the National Glucose Reference Method. From a 20-day replication study, we estimated within-run, between-run/within-day, between-run/between-day, and total standard deviations at three concentrations for each method. From duplicate analyses of 100 samples on the aca III, aca II, and other methods, we estimated the bias from the regression line at specific concentrations and total error from a tolerance limit about the regression line. Analytical performance of the aca III was judged acceptable because these estimated errors were small.", "contents": "DuPont aca III performance as tested according to NCCLS guidelines. We evaluated the analytical performance of 10 representative channels on the new microprocessor-controlled DuPont aca III according to the guidelines (draft documents PSEP-2, 3, and 4) proposed by the Instrument Evaluation Subcommittee of the National Committee for Clinical Laboratory Standards. These guidelines were used for the experimental design and data analysis for the precision and accuracy testing, the latter by comparison with an aca II, with results of atomic absorption spectroscopy for calcium, and with the National Glucose Reference Method. From a 20-day replication study, we estimated within-run, between-run/within-day, between-run/between-day, and total standard deviations at three concentrations for each method. From duplicate analyses of 100 samples on the aca III, aca II, and other methods, we estimated the bias from the regression line at specific concentrations and total error from a tolerance limit about the regression line. Analytical performance of the aca III was judged acceptable because these estimated errors were small."}
{"id": "PMID:476923", "title": "Metabolic balance of aluminum studied in six men.", "content": "The metabolism of aluminum was followed in patients who were receiving strictly controlled diets, either unaltered or supplemented with aluminum-containing antacids. Aluminum was determined by atomic absorption spectrophotometry with a graphite furnace. All food, water, and medications were analyzed, as were aliquots of urine and stool collected throughout six-day periods. Patients were usually studied for five consecutive six-day periods on the same diet and aluminum intake. This study included six patients who were studied for seven to 21 such six-day periods. During control periods, when the patients were receiving less than 5 mg of aluminum per day, the balance was usually slightly negative, but when the diet was supplemented with antacids to contain 1--3 g of aluminum per day, an average positive balance of 23 to 313 mg of aluminum per day was observed for the total time on the same intake. Consecutive six-day periods while a subject was receiving antacids might show either positive or negative balances, but the average retention for 18--84 days on antacids was always positive. Thus the potential effects of the storage of aluminum in the tissues must be considered in the chronic administration of antacids.", "contents": "Metabolic balance of aluminum studied in six men. The metabolism of aluminum was followed in patients who were receiving strictly controlled diets, either unaltered or supplemented with aluminum-containing antacids. Aluminum was determined by atomic absorption spectrophotometry with a graphite furnace. All food, water, and medications were analyzed, as were aliquots of urine and stool collected throughout six-day periods. Patients were usually studied for five consecutive six-day periods on the same diet and aluminum intake. This study included six patients who were studied for seven to 21 such six-day periods. During control periods, when the patients were receiving less than 5 mg of aluminum per day, the balance was usually slightly negative, but when the diet was supplemented with antacids to contain 1--3 g of aluminum per day, an average positive balance of 23 to 313 mg of aluminum per day was observed for the total time on the same intake. Consecutive six-day periods while a subject was receiving antacids might show either positive or negative balances, but the average retention for 18--84 days on antacids was always positive. Thus the potential effects of the storage of aluminum in the tissues must be considered in the chronic administration of antacids."}
{"id": "PMID:476924", "title": "The use of immobilized enzyme reactors in continuous-flow analyzers for the determination of glucose, urea, and uric acid.", "content": "We describe the use of immobilized enzymes in assay methods for the determination of glucose with glucose oxidase, uric acid with uricase, and urea with urease in serum samples. The enzyme reactor tubes were adapted to continuous-flow analyzers (Technicon AA II, SMA 12/60, and SMAC) used in routine laboratory determinations, and results with their use were compared to those from assays involving soluble enzymes. We substituted the reactors for the free enzyme reagents in the respective channels of the SMA 12/60 and SMAC, without modifying the parameters of the remaining channels. We compared assay sensitivity, precision, and carryover for immobilized and conventional liquid enzymes. Immobilized enzyme reactors provide accurate, reliable, convenient, and economical alternatives to the use of free enzyme reagents in these systems.", "contents": "The use of immobilized enzyme reactors in continuous-flow analyzers for the determination of glucose, urea, and uric acid. We describe the use of immobilized enzymes in assay methods for the determination of glucose with glucose oxidase, uric acid with uricase, and urea with urease in serum samples. The enzyme reactor tubes were adapted to continuous-flow analyzers (Technicon AA II, SMA 12/60, and SMAC) used in routine laboratory determinations, and results with their use were compared to those from assays involving soluble enzymes. We substituted the reactors for the free enzyme reagents in the respective channels of the SMA 12/60 and SMAC, without modifying the parameters of the remaining channels. We compared assay sensitivity, precision, and carryover for immobilized and conventional liquid enzymes. Immobilized enzyme reactors provide accurate, reliable, convenient, and economical alternatives to the use of free enzyme reagents in these systems."}
{"id": "PMID:476925", "title": "Rapid analysis for iodotyrosines and iodothyronines in thyroglobulin by reversed-phase liquid chromatography.", "content": "We describe a 10-min reversed-phase \"high-pressure' liquid-chromatographic procedure for measuring tyrosine, monoiodotyrosine, diiodotyrosine, 3,5-diiodothyronine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine, and thyroxine. Resolution and quantitation of a mixture of these amino acids were excellent on LiChrosorb (Altex) RP-8 with isocratic elution (1.5 mL/min) with acetonitrile/water/glacial acetic acid (50/49/1 by vol). As little as 100 ng of each iodoamino acid could be detected and quantitated with a conventional 1-cm, flow-through spectrophotometric (254-nm) detector coupled to a 10-mV strip-chart recorder. Analyses for monoiodotyrosine, diiodotyrosine, 3,5,3'-triiodothyronine, and thyroxine in hog and beef thyroglobulin hydrolysates (sequential digestion with pronase and aminopeptidase) agreed well with results by anion-exchange chromatography and by competitive radioassays. To prevent interference by tryptophan in the analysis for diiodotyrosine, we batch-separated the iodoamino acids by anion-exchange chromatography before the procedure. The procedure we describe seems generally useful for detection and quantitation of thyroid hormones, thyroid hormone metabolites, and iodotyrosines.", "contents": "Rapid analysis for iodotyrosines and iodothyronines in thyroglobulin by reversed-phase liquid chromatography. We describe a 10-min reversed-phase \"high-pressure' liquid-chromatographic procedure for measuring tyrosine, monoiodotyrosine, diiodotyrosine, 3,5-diiodothyronine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine, and thyroxine. Resolution and quantitation of a mixture of these amino acids were excellent on LiChrosorb (Altex) RP-8 with isocratic elution (1.5 mL/min) with acetonitrile/water/glacial acetic acid (50/49/1 by vol). As little as 100 ng of each iodoamino acid could be detected and quantitated with a conventional 1-cm, flow-through spectrophotometric (254-nm) detector coupled to a 10-mV strip-chart recorder. Analyses for monoiodotyrosine, diiodotyrosine, 3,5,3'-triiodothyronine, and thyroxine in hog and beef thyroglobulin hydrolysates (sequential digestion with pronase and aminopeptidase) agreed well with results by anion-exchange chromatography and by competitive radioassays. To prevent interference by tryptophan in the analysis for diiodotyrosine, we batch-separated the iodoamino acids by anion-exchange chromatography before the procedure. The procedure we describe seems generally useful for detection and quantitation of thyroid hormones, thyroid hormone metabolites, and iodotyrosines."}
{"id": "PMID:476926", "title": "Spurious hyperbilirubinemia in uremic patients on propranolol therapy.", "content": "Chronic, low-grade, unexplained increases of total serum bilirubin concentration were observed in 16 of 132 (12%) chronic dialysis patients. Cessation of antihypertensive medication in four patients resulted in disappearance of hyperbilirubinemia. Propranolol was the only antihypertensive drug common to all 16 patients. Daily dosage varied among the patients, but the mean dose was 308 mg (+/- 51 SEM). Serial determinations of sera from individual patients given different doses and from the group as a whole demonstrated a linear relationship between propranolol dose and apparent total serum bilirubin concentration with continuous-flow analysis. When serum specimens from uremic patients receiving propranolol were treated with diazotized fulfanilic acid and examined spectrophotometrically, an absorbance peak distinct from but overlapping that of bilirubin was consistently demonstrated. The material producing the peak disappeared when the drug was stopped, did not dialyze, and was not reproduced by the in vitro addition of propranolol to uremic serum. We postulate that a metabolite(s) of propranolol is retained in uremic serum and interferes with the bilirubin determination.", "contents": "Spurious hyperbilirubinemia in uremic patients on propranolol therapy. Chronic, low-grade, unexplained increases of total serum bilirubin concentration were observed in 16 of 132 (12%) chronic dialysis patients. Cessation of antihypertensive medication in four patients resulted in disappearance of hyperbilirubinemia. Propranolol was the only antihypertensive drug common to all 16 patients. Daily dosage varied among the patients, but the mean dose was 308 mg (+/- 51 SEM). Serial determinations of sera from individual patients given different doses and from the group as a whole demonstrated a linear relationship between propranolol dose and apparent total serum bilirubin concentration with continuous-flow analysis. When serum specimens from uremic patients receiving propranolol were treated with diazotized fulfanilic acid and examined spectrophotometrically, an absorbance peak distinct from but overlapping that of bilirubin was consistently demonstrated. The material producing the peak disappeared when the drug was stopped, did not dialyze, and was not reproduced by the in vitro addition of propranolol to uremic serum. We postulate that a metabolite(s) of propranolol is retained in uremic serum and interferes with the bilirubin determination."}
{"id": "PMID:476927", "title": "Radiochemical method for measuring plasma clearance and urinary excretion of pteroylglutamic acid.", "content": "A radiochemical procedure is described for specific determination of pteroylglutamate in serum and urine. This method depends on denaturation of methyltetrahydrofolate with peroxide and measurement of the residual folate by a ligand-binding radioassay. The binding determinant for the radioassay is a folate-binding protein, partially purified from chronic myelogenous lekemia cells, that has low affinity for the reduced folates and thus will preferentially measure residual pteroylglutamate rather than any nondenatured residual methyltetrahydrofolate. We used this assay to measure the clearance from plasma and the urinary excretion of pteroylglutamate and a small fraction of serum folate that is stable to this oxidation procedure. The plasma clearance after intravenous injection is characterized by an initial rapid distribution phase followed by a second, slower metabolic phase; after about 2 h all of the administered pteroylglutamate has been cleared from the blood. The peak concentration of total folate in serum 1--2 min after administration of pteroylglutamate exceeded the sum of the endogenous stable and baseline serum folate, indicating that a reduced labile folate was released from the liver and perhaps from other tissues. This reduced folate had a slower metabolic clearance rate and was excreted to some extent in urine. Only 2.3 and 7.9% of the pteroylglutamate administered to two normal subjects was excreted as stable folate.", "contents": "Radiochemical method for measuring plasma clearance and urinary excretion of pteroylglutamic acid. A radiochemical procedure is described for specific determination of pteroylglutamate in serum and urine. This method depends on denaturation of methyltetrahydrofolate with peroxide and measurement of the residual folate by a ligand-binding radioassay. The binding determinant for the radioassay is a folate-binding protein, partially purified from chronic myelogenous lekemia cells, that has low affinity for the reduced folates and thus will preferentially measure residual pteroylglutamate rather than any nondenatured residual methyltetrahydrofolate. We used this assay to measure the clearance from plasma and the urinary excretion of pteroylglutamate and a small fraction of serum folate that is stable to this oxidation procedure. The plasma clearance after intravenous injection is characterized by an initial rapid distribution phase followed by a second, slower metabolic phase; after about 2 h all of the administered pteroylglutamate has been cleared from the blood. The peak concentration of total folate in serum 1--2 min after administration of pteroylglutamate exceeded the sum of the endogenous stable and baseline serum folate, indicating that a reduced labile folate was released from the liver and perhaps from other tissues. This reduced folate had a slower metabolic clearance rate and was excreted to some extent in urine. Only 2.3 and 7.9% of the pteroylglutamate administered to two normal subjects was excreted as stable folate."}
{"id": "PMID:476928", "title": "Measurement of acetate in human blood by gas chromatography: effects of sample preparation, feeding, and various diseases.", "content": "We measured acetate concentrations in whole blood, serum, and plasma by a modification of a previously described method involving vacuum distillation and gas chromatography. The mean acetate concentration of fresh venous plasma from 27 normal subjects was 51 +/- 5 mumol/L (95% confidence limits ranged from 0 to 103 mumol/L). The acetate concentrations of serum and plasma incubated for 2 h at either 4 degrees C or 27 degrees C were the same. The acetate concentration of whole blood incubated at 27 degrees C was significantly greater than that of blood incubated at 4 degrees C. This change may have resulted from the production of acetate by erythrocytes or from the hydrolysis of acetate esters. Storage of plasma at -20 degrees C for 24 h significantly increased acetate concentrations from 26 +/- 6 mumol/L to 63 +/- 4 mumol/L. After the subjects consumed a standard breakfast, venous plasma acetate concentrations increased from 58 to 97 mumol/L at 30 min. Acetate concentrations in arterial plasma exceeded those in venous plasma. Plasma acetate concentrations were not significantly altered in patients with malignancy or diabetes mellitus, but severe liver disease and severe acidosis were both associated with increased acetate concentrations. These preliminary observations suggest that plasma acetate concentrations may be altered in several disease states.", "contents": "Measurement of acetate in human blood by gas chromatography: effects of sample preparation, feeding, and various diseases. We measured acetate concentrations in whole blood, serum, and plasma by a modification of a previously described method involving vacuum distillation and gas chromatography. The mean acetate concentration of fresh venous plasma from 27 normal subjects was 51 +/- 5 mumol/L (95% confidence limits ranged from 0 to 103 mumol/L). The acetate concentrations of serum and plasma incubated for 2 h at either 4 degrees C or 27 degrees C were the same. The acetate concentration of whole blood incubated at 27 degrees C was significantly greater than that of blood incubated at 4 degrees C. This change may have resulted from the production of acetate by erythrocytes or from the hydrolysis of acetate esters. Storage of plasma at -20 degrees C for 24 h significantly increased acetate concentrations from 26 +/- 6 mumol/L to 63 +/- 4 mumol/L. After the subjects consumed a standard breakfast, venous plasma acetate concentrations increased from 58 to 97 mumol/L at 30 min. Acetate concentrations in arterial plasma exceeded those in venous plasma. Plasma acetate concentrations were not significantly altered in patients with malignancy or diabetes mellitus, but severe liver disease and severe acidosis were both associated with increased acetate concentrations. These preliminary observations suggest that plasma acetate concentrations may be altered in several disease states."}
{"id": "PMID:476929", "title": "Measurement of 4-hydroxyphenylacetic aciduria as a screening test for small-bowel disease.", "content": "We evaluted measurement of urinary 4-hydroxyphenyl acetic acid as a potential screening method for small-bowel disease and bacterial overgrowth syndromes in 360 unselected acutely ill infants and children. Control data were obtained on 120 healthy children, ages 1.5 to 15 years, from a general medical practice, 48 healthy infants, ages one to five years, from local day nurseries, and 150 healthy babies, ages less than one to eight days. Comparative data were from 300 acutely ill hospitalized babies and children, ranging in age from less than one day to 15 years and without clinical evidence for small-bowel disease and bacterial overgrowth syndrome. No false-negative results and only 2% false-positive results were observed. Among the 10 patients whose urinary excretion of the analyte was considered to be abnormal were patients with Giardia lamblia infestation, ileal resection with blind loop, and other diseases of the small intestine associated with bacterial overgrowth. We conclude that measurement of 4-hydroxyphenylacetic acid excretion is useful in screening for such diseases.", "contents": "Measurement of 4-hydroxyphenylacetic aciduria as a screening test for small-bowel disease. We evaluted measurement of urinary 4-hydroxyphenyl acetic acid as a potential screening method for small-bowel disease and bacterial overgrowth syndromes in 360 unselected acutely ill infants and children. Control data were obtained on 120 healthy children, ages 1.5 to 15 years, from a general medical practice, 48 healthy infants, ages one to five years, from local day nurseries, and 150 healthy babies, ages less than one to eight days. Comparative data were from 300 acutely ill hospitalized babies and children, ranging in age from less than one day to 15 years and without clinical evidence for small-bowel disease and bacterial overgrowth syndrome. No false-negative results and only 2% false-positive results were observed. Among the 10 patients whose urinary excretion of the analyte was considered to be abnormal were patients with Giardia lamblia infestation, ileal resection with blind loop, and other diseases of the small intestine associated with bacterial overgrowth. We conclude that measurement of 4-hydroxyphenylacetic acid excretion is useful in screening for such diseases."}
{"id": "PMID:476930", "title": "Increase in dialyzable calcium associated with therapy with lithium.", "content": "We measured total and dialyzable calcium concentrations in consecutive sera submitted for routine lithium analysis. Of 98 samples from 61 different individuals, six (6.1%) total calcium results and 32 (33%) dialyzable calcium results were above the respective reference intervals. By comparison, when both total and dialyzable calcium were measured on 50 different apparently healthy volunteers, no results were outside either reference interval (2.20--2.58 mmol/L for total and 1.30--1.47 mmol/L for dialyzable calcium). These increases were not due to age or sex differences between the patients and controls. From the dialyzable calcium data, there appears to be an even higher incidence of mild hypercalcemia in patients receiving oral lithium salts than is indicated by the total calcium concentration alone.", "contents": "Increase in dialyzable calcium associated with therapy with lithium. We measured total and dialyzable calcium concentrations in consecutive sera submitted for routine lithium analysis. Of 98 samples from 61 different individuals, six (6.1%) total calcium results and 32 (33%) dialyzable calcium results were above the respective reference intervals. By comparison, when both total and dialyzable calcium were measured on 50 different apparently healthy volunteers, no results were outside either reference interval (2.20--2.58 mmol/L for total and 1.30--1.47 mmol/L for dialyzable calcium). These increases were not due to age or sex differences between the patients and controls. From the dialyzable calcium data, there appears to be an even higher incidence of mild hypercalcemia in patients receiving oral lithium salts than is indicated by the total calcium concentration alone."}
{"id": "PMID:476932", "title": "Adsorption of organic acids from amniotic fluid and urine onto silica gel before analysis by gas chromatography and combined gas chromatography/mass spectrometry.", "content": "We describe a method for adsorbing organic acids from amniotic fluid and urine specimens onto a column of silica gel before analysis by gas chromatography and by combined gas chromatography/mass spectrometry. Analytical recoveries of individual organic acids by our adsorption method compare favorably with those obtained by a more laborious manual extraction with ethyl acetate. Results for specimens of amniotic fluid and urine show that our method gives better recoveries of most of the organic acids. Furthermore, because citric acid is also extracted in our method without extracting significant amounts of sulfate or phosphate, the method is a suitable alternative to methods involving the use of DEAE-Sephadex for concentrating these acids.", "contents": "Adsorption of organic acids from amniotic fluid and urine onto silica gel before analysis by gas chromatography and combined gas chromatography/mass spectrometry. We describe a method for adsorbing organic acids from amniotic fluid and urine specimens onto a column of silica gel before analysis by gas chromatography and by combined gas chromatography/mass spectrometry. Analytical recoveries of individual organic acids by our adsorption method compare favorably with those obtained by a more laborious manual extraction with ethyl acetate. Results for specimens of amniotic fluid and urine show that our method gives better recoveries of most of the organic acids. Furthermore, because citric acid is also extracted in our method without extracting significant amounts of sulfate or phosphate, the method is a suitable alternative to methods involving the use of DEAE-Sephadex for concentrating these acids."}
{"id": "PMID:476933", "title": "Washington University Case Conference. Multiple myeloma and hypercalcemia?", "content": "We present a case of multiple myeloma with high values for total calcium. The case discussion focuses on the role of the laboratory in diagnosis and monitoring of multiple myeloma and on the causes of the associated hypercalcemia. We present evidence for calcium binding to the paraprotein as a cause for the \"hypercalcemia\" in this case and we document a difference in the results for total calcium among various methods when this patient's serum was analyzed.", "contents": "Washington University Case Conference. Multiple myeloma and hypercalcemia? We present a case of multiple myeloma with high values for total calcium. The case discussion focuses on the role of the laboratory in diagnosis and monitoring of multiple myeloma and on the causes of the associated hypercalcemia. We present evidence for calcium binding to the paraprotein as a cause for the \"hypercalcemia\" in this case and we document a difference in the results for total calcium among various methods when this patient's serum was analyzed."}
{"id": "PMID:476934", "title": "Three different radioiodination methods for human spleen ferritin compared.", "content": "We compared three different methods for radioiodination of human spleen ferritin. Tracers prepared by direct oxidative iodination with use of Chloramine T or 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (Iodogen) were structurally altered by the labeling procedure, as was made apparent by gel filtration and electrophoresis on polyacrylamide gel. Tracers prepared by conjugation to radioiodinated N-succinimidyl-6-(4-hydroxyphenyl)propionate retained their structural integrity, as assessed by physicochemical methods. Such tracers bound more than 90% in antibody excess, yielded self-displacement curves parallel to unlabeled ferritin standards, and retained their immunoreactivity for nine weeks. By contrast, immunological activity and stability of tracers prepared by the oxidative methods were less satisfactory. Tracers prepared by using lodogen were unsuitable for use in the ferritin radioimmunoassay because of shallow standard-inhibition curves and poor binding in the absence of added standard. Thus, we found the conjugation procedure to be the most suitable of the three methods for preparation of spleen-ferritin tracers.", "contents": "Three different radioiodination methods for human spleen ferritin compared. We compared three different methods for radioiodination of human spleen ferritin. Tracers prepared by direct oxidative iodination with use of Chloramine T or 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (Iodogen) were structurally altered by the labeling procedure, as was made apparent by gel filtration and electrophoresis on polyacrylamide gel. Tracers prepared by conjugation to radioiodinated N-succinimidyl-6-(4-hydroxyphenyl)propionate retained their structural integrity, as assessed by physicochemical methods. Such tracers bound more than 90% in antibody excess, yielded self-displacement curves parallel to unlabeled ferritin standards, and retained their immunoreactivity for nine weeks. By contrast, immunological activity and stability of tracers prepared by the oxidative methods were less satisfactory. Tracers prepared by using lodogen were unsuitable for use in the ferritin radioimmunoassay because of shallow standard-inhibition curves and poor binding in the absence of added standard. Thus, we found the conjugation procedure to be the most suitable of the three methods for preparation of spleen-ferritin tracers."}
{"id": "PMID:476935", "title": "Evaluation of the DuPont aca amylase method.", "content": "The dilution/activity curve for the DuPont aca kinetic amylase (EC 3.2.1.1) method is linear to approximately six times the upper limit of the expected range. Total reaction time on the aca is 4.3 min; however, there is a nonlinear rate of change in absorbance for at least the first 8 min, which is consistent with a lag phase. The within-run and day-to-day coefficients of variation were 2.4% at 100 U/L and 3.6% at 114 U/L, respectively. There was no interference by marked hemolysis (hemoglobin 4 g/L) or by glucose concentrations of 10--50 g/L. Comparisons by the split-sample method between the Amylochrome procedure and the aca procedure gave correlation coefficients of 0.992 for serum and 0.989 for urine. A second comparison, between the AutoAnalyzer I method and the aca, gave correlation coefficients of 0.998 for serum and 0.909 for urine. The expected range for serum was 18--106 U/L and for urine 2--33 U/2 h.", "contents": "Evaluation of the DuPont aca amylase method. The dilution/activity curve for the DuPont aca kinetic amylase (EC 3.2.1.1) method is linear to approximately six times the upper limit of the expected range. Total reaction time on the aca is 4.3 min; however, there is a nonlinear rate of change in absorbance for at least the first 8 min, which is consistent with a lag phase. The within-run and day-to-day coefficients of variation were 2.4% at 100 U/L and 3.6% at 114 U/L, respectively. There was no interference by marked hemolysis (hemoglobin 4 g/L) or by glucose concentrations of 10--50 g/L. Comparisons by the split-sample method between the Amylochrome procedure and the aca procedure gave correlation coefficients of 0.992 for serum and 0.989 for urine. A second comparison, between the AutoAnalyzer I method and the aca, gave correlation coefficients of 0.998 for serum and 0.909 for urine. The expected range for serum was 18--106 U/L and for urine 2--33 U/2 h."}
{"id": "PMID:476938", "title": "Modification of the DuPont aca as exemplified by a new procedure for neonatal total bilirubin.", "content": "Hemoglobin interferes with determination of total bilirubin by the diazo procedure with the DuPont aca. We compared results by that procedure with those by a manual direct spectrophotometric two-wavelength method. Because the manual method was not inhibited by hemolysis as was the DuPont aca diazo procedure, we reprogrammed our DuPont aca to measure the absorbance of diluted serum samples at 452 and 577 nm with use of DuPont absorbance packs as the sample pack and cuvette. Results correlated well with those by the manual spectrophotometric method, and hemolysis no longer interfered. The method is primarily intended for use with serum from neonates.", "contents": "Modification of the DuPont aca as exemplified by a new procedure for neonatal total bilirubin. Hemoglobin interferes with determination of total bilirubin by the diazo procedure with the DuPont aca. We compared results by that procedure with those by a manual direct spectrophotometric two-wavelength method. Because the manual method was not inhibited by hemolysis as was the DuPont aca diazo procedure, we reprogrammed our DuPont aca to measure the absorbance of diluted serum samples at 452 and 577 nm with use of DuPont absorbance packs as the sample pack and cuvette. Results correlated well with those by the manual spectrophotometric method, and hemolysis no longer interfered. The method is primarily intended for use with serum from neonates."}
{"id": "PMID:476936", "title": "Fluorometric measurement of theophylline.", "content": "We have developed a simple, sensitive procedure in which theophylline in a solution of cerium(IV) acts as an oxidizable compound to produce fluorescent cerium(III) when combined with a microsolvent for extraction at pH 7.9. This method provides excellent sensitivity and specificity because other xanthines and phenobarbital, secobarbital, amobarbital, and phenytoin do not interfere. Day-to-day reproducibility (coefficient of variation, 6%) is attained for theophylline concentrations of 10 and 20 mg/L. Results correlate well with liquid chromatography and enzyme immunoassay procedures. Total analysis time for a single sample is 10 min.", "contents": "Fluorometric measurement of theophylline. We have developed a simple, sensitive procedure in which theophylline in a solution of cerium(IV) acts as an oxidizable compound to produce fluorescent cerium(III) when combined with a microsolvent for extraction at pH 7.9. This method provides excellent sensitivity and specificity because other xanthines and phenobarbital, secobarbital, amobarbital, and phenytoin do not interfere. Day-to-day reproducibility (coefficient of variation, 6%) is attained for theophylline concentrations of 10 and 20 mg/L. Results correlate well with liquid chromatography and enzyme immunoassay procedures. Total analysis time for a single sample is 10 min."}
{"id": "PMID:476937", "title": "Comparison of theophylline concentrations in venous blood and capillary blood in asthmatic children.", "content": "Twenty children, five to 16 years old, diagnosed as having asthma, were studied to compare the serum concentrations of theophylline obtained by standard venipuncture with capillary concentrations obtained by finger lancet. The mean theophylline concentration in venous samples was 9.27 mg/L and in capillary samples was 9.26 mg/L. Comparison by the paired t-test showed no statistical difference between kinds of samples. When surveyed as to their preference, nine patients preferred the venous method, five the capillary method, and six had no preference.", "contents": "Comparison of theophylline concentrations in venous blood and capillary blood in asthmatic children. Twenty children, five to 16 years old, diagnosed as having asthma, were studied to compare the serum concentrations of theophylline obtained by standard venipuncture with capillary concentrations obtained by finger lancet. The mean theophylline concentration in venous samples was 9.27 mg/L and in capillary samples was 9.26 mg/L. Comparison by the paired t-test showed no statistical difference between kinds of samples. When surveyed as to their preference, nine patients preferred the venous method, five the capillary method, and six had no preference."}
{"id": "PMID:476939", "title": "A commercial digoxin radioassay modified for use as an emergency (\"stat\") procedure.", "content": "A recent \"stat\" procedure recommended by the manufacturer for use with a commercial digoxin radioassay involves doubling the amount of antibody used in the regular procedure, to compensate for the decrease in binding that results from use of a shorter (10 min) incubation. This has two obvious disadvantages: increased material cost and decreased sensitivity because of the increase in the binding capacity of the assay. In our modified procedure, we kept the binding capacity constant by decreasing the volume of the incubation mixture without increasing the amount of antibody. Total binding and the rate of binding on 10-min incubation are 50.4 and less than 1.3% per minute, respectively. Results by our modified stat procedure compare well with those by the manufacturer's suggested regular procedure in terms of sensitivity, accuracy, and precision, and are more sensitive and economical than those by the manufacturer's suggested stat procedure.", "contents": "A commercial digoxin radioassay modified for use as an emergency (\"stat\") procedure. A recent \"stat\" procedure recommended by the manufacturer for use with a commercial digoxin radioassay involves doubling the amount of antibody used in the regular procedure, to compensate for the decrease in binding that results from use of a shorter (10 min) incubation. This has two obvious disadvantages: increased material cost and decreased sensitivity because of the increase in the binding capacity of the assay. In our modified procedure, we kept the binding capacity constant by decreasing the volume of the incubation mixture without increasing the amount of antibody. Total binding and the rate of binding on 10-min incubation are 50.4 and less than 1.3% per minute, respectively. Results by our modified stat procedure compare well with those by the manufacturer's suggested regular procedure in terms of sensitivity, accuracy, and precision, and are more sensitive and economical than those by the manufacturer's suggested stat procedure."}
{"id": "PMID:476940", "title": "Evaluation of the BMC glucose oxidase/peroxidase-4-aminophenazone-phenol procedure for glucose as adapted to the Technicon SMAC.", "content": "We evaluated the analytical performance of Trinder's glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) 4-aminophenazone-phenol method for the quantification of serum glucose as adapted to the Technicon SMAC. Our results correlated well with those by the routine SMAC glucose oxidase/peroxidase 3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline method (y = 1.02x - 49.4; r = 0.99) and the glucose oxidase oxygen-rate method (y = 0.99x + 14; r = 0.99) with the Beckman Glucose Analyzer. Sample-to-sample interaction was less than 1%. Ascorbic acid or uric acid in concentrations as high as 200 mg/L were without demonstrable effect on results for glucose. Intra- and inter-assay precisions (CV) were 1.6 and 2.3%, respectively. The upper limit of linearity was about 5 g/L. Adaptation of the Trinder method for glucose to the SMAC is simple and provides an analytically acceptable and economical alternative to the methods ordinarily used with the SMAC.", "contents": "Evaluation of the BMC glucose oxidase/peroxidase-4-aminophenazone-phenol procedure for glucose as adapted to the Technicon SMAC. We evaluated the analytical performance of Trinder's glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) 4-aminophenazone-phenol method for the quantification of serum glucose as adapted to the Technicon SMAC. Our results correlated well with those by the routine SMAC glucose oxidase/peroxidase 3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline method (y = 1.02x - 49.4; r = 0.99) and the glucose oxidase oxygen-rate method (y = 0.99x + 14; r = 0.99) with the Beckman Glucose Analyzer. Sample-to-sample interaction was less than 1%. Ascorbic acid or uric acid in concentrations as high as 200 mg/L were without demonstrable effect on results for glucose. Intra- and inter-assay precisions (CV) were 1.6 and 2.3%, respectively. The upper limit of linearity was about 5 g/L. Adaptation of the Trinder method for glucose to the SMAC is simple and provides an analytically acceptable and economical alternative to the methods ordinarily used with the SMAC."}
{"id": "PMID:476942", "title": "Myocardial infarction?--a conflict between electrocardiographic changes and biochemical data.", "content": "We present a case of hypovolemic shock accompanied by electrocardiographic changes classically associated with acute myocardial infarction. Prompt therapeutic intervention, which included correction of the hypovolemic shock, resulted in stabilization of the patient's clinical course, increased activities of the cardiac-specific enzymes in serum were not documented. Serial electrophoretic determinations of the isoenzymes of serum lactate dehydrogenase did not show the characteristic changes associated with myocardial infarction. Accurate determination of the serum isoenzymes provided valuable diagnostic information which, accompanied by the patient's clinical improvement, militated strongly against the occurrence of myocardial infarction.", "contents": "Myocardial infarction?--a conflict between electrocardiographic changes and biochemical data. We present a case of hypovolemic shock accompanied by electrocardiographic changes classically associated with acute myocardial infarction. Prompt therapeutic intervention, which included correction of the hypovolemic shock, resulted in stabilization of the patient's clinical course, increased activities of the cardiac-specific enzymes in serum were not documented. Serial electrophoretic determinations of the isoenzymes of serum lactate dehydrogenase did not show the characteristic changes associated with myocardial infarction. Accurate determination of the serum isoenzymes provided valuable diagnostic information which, accompanied by the patient's clinical improvement, militated strongly against the occurrence of myocardial infarction."}
{"id": "PMID:476953", "title": "Automated analysis of hydroxyproline with elimination of non-specific reacting substances.", "content": "A modified urinary hydroxyproline method is presented which utilizes an automated technique, Technicon instrumentation and Ehrlich's reagent. Acid hydrolysates are neutralized automatically. Non-specific reacting substances are determined simultaneously with each sample and subtracted as a blank. This method has been found to be superior to the use of charcoal-resin as an absorbent. After blank correction, the mean hydroxyproline excretion of normal and osteoporotic patients are similar. In all groups studied, including Paget's disease, the blanks reresent a significant fraction of total reacting substances and show wide variation between samples. Multiple samples from patients over a two year period show little variation in hydroxyproline excretion when corrected for non-specific reacting substances.", "contents": "Automated analysis of hydroxyproline with elimination of non-specific reacting substances. A modified urinary hydroxyproline method is presented which utilizes an automated technique, Technicon instrumentation and Ehrlich's reagent. Acid hydrolysates are neutralized automatically. Non-specific reacting substances are determined simultaneously with each sample and subtracted as a blank. This method has been found to be superior to the use of charcoal-resin as an absorbent. After blank correction, the mean hydroxyproline excretion of normal and osteoporotic patients are similar. In all groups studied, including Paget's disease, the blanks reresent a significant fraction of total reacting substances and show wide variation between samples. Multiple samples from patients over a two year period show little variation in hydroxyproline excretion when corrected for non-specific reacting substances."}
{"id": "PMID:476954", "title": "A new sensitive method for assay of histidase in human skin and detection of heterozygotes for histidinemia.", "content": "A new sensitive method was developed for assay of histidase in the stratum corneum of human skin with [14C] histidine as substrate. With this method histidase could be detected using less than 1 mg of stratum corneum. Skin histidase was markedly reduced in seven infants with histidinemia. The enzyme activities in thirteen parents of the patients were about 50% of the normal values, and even the highest values in the parents did not overlap the lowest normal values. Thus heterozygotes in the autosomal recessive form of histidinemia can be distinguished from normal persons by their skin histidase activity measured by this sensitive assay method.", "contents": "A new sensitive method for assay of histidase in human skin and detection of heterozygotes for histidinemia. A new sensitive method was developed for assay of histidase in the stratum corneum of human skin with [14C] histidine as substrate. With this method histidase could be detected using less than 1 mg of stratum corneum. Skin histidase was markedly reduced in seven infants with histidinemia. The enzyme activities in thirteen parents of the patients were about 50% of the normal values, and even the highest values in the parents did not overlap the lowest normal values. Thus heterozygotes in the autosomal recessive form of histidinemia can be distinguished from normal persons by their skin histidase activity measured by this sensitive assay method."}
{"id": "PMID:476955", "title": "Automated determination of total plasma cholesterol: a serum calibration technique.", "content": "The automated (AutoAnalyzer II) determination of cholesterol in nine serum pools in the concentration range 3.465-8.871 mmol/l, gave results that were approximately 10% higher than reference values when the analyses were based on unesterified cholesterol standards containing the same amount of water as the sample extracts (1963 analyses in 12 laboratories during a 12 month period; automated value = 0.032 + 1.10 X (Reference value)). A serum calibration procedure was successful ilues, and was equally effective in correcting the values observed for aliquots of 368 fresh-frozen plasma samples analyzed in each of the 12 laboratories during a 38 month period.", "contents": "Automated determination of total plasma cholesterol: a serum calibration technique. The automated (AutoAnalyzer II) determination of cholesterol in nine serum pools in the concentration range 3.465-8.871 mmol/l, gave results that were approximately 10% higher than reference values when the analyses were based on unesterified cholesterol standards containing the same amount of water as the sample extracts (1963 analyses in 12 laboratories during a 12 month period; automated value = 0.032 + 1.10 X (Reference value)). A serum calibration procedure was successful ilues, and was equally effective in correcting the values observed for aliquots of 368 fresh-frozen plasma samples analyzed in each of the 12 laboratories during a 38 month period."}
{"id": "PMID:476956", "title": "Comparison of platelet count and platelet protein methods for determination of platelet MAO activity.", "content": "Platelet monoamine oxidase (MAO) activity in 10 normal volunteers was studied as a function of platelet protein or electronically-determined platelet counts. Comparisons of the two methods were made for samples assayed on the same day as well as one week later. The MAO activities resulting from both methods were significantly correlated and reproducible but the results of the platelet count method were, in most instances, slightly but significantly more reliable than the platelet protein method. The relevance of these results to the controversy concerning platelet MAO activity in schizophrenia is discussed.", "contents": "Comparison of platelet count and platelet protein methods for determination of platelet MAO activity. Platelet monoamine oxidase (MAO) activity in 10 normal volunteers was studied as a function of platelet protein or electronically-determined platelet counts. Comparisons of the two methods were made for samples assayed on the same day as well as one week later. The MAO activities resulting from both methods were significantly correlated and reproducible but the results of the platelet count method were, in most instances, slightly but significantly more reliable than the platelet protein method. The relevance of these results to the controversy concerning platelet MAO activity in schizophrenia is discussed."}
{"id": "PMID:476959", "title": "A new enzymatic method for the determination of glycollate in urine and plasma.", "content": "An enzymatic assay for the measurement of glycollate in urine and plasma is described. Gycollic acid oxidase (glycollate:oxygen oxidoreductase, EC 1.1.3), extracted from spinach leaves, is used in the enzymatic oxidation of glycollate to produce glyoxylate and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide oxidatively couples sulphonated 2,4-dichlorophenol and 4-aminophenazone to form a soluble purple quinoneimine dye. Interfering substances are removed from plasma by deproteinisation and from urine by adsorption onto activated charcoal before analysis. Glycollic acid oxidase also catalyses the oxidation of lactic acid which therefore has to be determined separately. The mean normal urinary glycollate for adults is 0.19 mmol/24 h with no difference between males and females. The mean normal plasma glycollate for adults is 0.17 mmol/l, but higher in males than in females.", "contents": "A new enzymatic method for the determination of glycollate in urine and plasma. An enzymatic assay for the measurement of glycollate in urine and plasma is described. Gycollic acid oxidase (glycollate:oxygen oxidoreductase, EC 1.1.3), extracted from spinach leaves, is used in the enzymatic oxidation of glycollate to produce glyoxylate and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide oxidatively couples sulphonated 2,4-dichlorophenol and 4-aminophenazone to form a soluble purple quinoneimine dye. Interfering substances are removed from plasma by deproteinisation and from urine by adsorption onto activated charcoal before analysis. Glycollic acid oxidase also catalyses the oxidation of lactic acid which therefore has to be determined separately. The mean normal urinary glycollate for adults is 0.19 mmol/24 h with no difference between males and females. The mean normal plasma glycollate for adults is 0.17 mmol/l, but higher in males than in females."}
{"id": "PMID:476960", "title": "The urinary excretions of iron and chromium by children with burns and scalds.", "content": "There is a wide variation in the urinary output of chromium by children who have been burned or scalded. It seems possible that when the excretion of chromium is increased this may be related to an increased plasma concentration of glucose which may follow injury, and when the excretion of chromium is reduced this may be related to a low body content of chromium. During the first seven days after injury there is a significant increase in the excretion of iron. Likewise the iron/chromium ratio is also raised over the first seven days. The overall loss of iron during the first twelve days or thereabouts is probably not sufficient to require nutritional supplements over this period.", "contents": "The urinary excretions of iron and chromium by children with burns and scalds. There is a wide variation in the urinary output of chromium by children who have been burned or scalded. It seems possible that when the excretion of chromium is increased this may be related to an increased plasma concentration of glucose which may follow injury, and when the excretion of chromium is reduced this may be related to a low body content of chromium. During the first seven days after injury there is a significant increase in the excretion of iron. Likewise the iron/chromium ratio is also raised over the first seven days. The overall loss of iron during the first twelve days or thereabouts is probably not sufficient to require nutritional supplements over this period."}
{"id": "PMID:476961", "title": "Acquired pyruvate kinase deficiency. The effect of maleic acid upon human erythrocyte pyruvate kinase.", "content": "1. Maleic acid is shown to be able to bind the thiol compound 2-mercaptoethanol. This is fully consistent with the data of Morgan and Friedman (1938). 2. Human erythrocyte pyruvate kinase dissolved and quantitated in Tris-maleate shows a loss of positive homotropic interactions, as compared to the same preparation in Tris-HCl. Hill coefficients (n) of n = 1.0-1.2 and n = 1.6-1.8 are obtained in Tris-maleate and Tris-HCl respectively. Half saturation [S] 0.5 and Vmax remain unchanged. Pyruvate kinase in Tris-maleate is slightly more stable to heating at 60 degrees C than in Tris-HCl. Incubation of the enzyme in Tris-maleate for one h with high concentrations of dithiotreitol restores the positive homotropic interactions. 3. It is proposed, that the abnormalities of the pyruvate kinase of some patients with acquired pyruvate kinase deficiency, obtained from a study in Tris-maleate, may partly be induced by the buffer itself.", "contents": "Acquired pyruvate kinase deficiency. The effect of maleic acid upon human erythrocyte pyruvate kinase. 1. Maleic acid is shown to be able to bind the thiol compound 2-mercaptoethanol. This is fully consistent with the data of Morgan and Friedman (1938). 2. Human erythrocyte pyruvate kinase dissolved and quantitated in Tris-maleate shows a loss of positive homotropic interactions, as compared to the same preparation in Tris-HCl. Hill coefficients (n) of n = 1.0-1.2 and n = 1.6-1.8 are obtained in Tris-maleate and Tris-HCl respectively. Half saturation [S] 0.5 and Vmax remain unchanged. Pyruvate kinase in Tris-maleate is slightly more stable to heating at 60 degrees C than in Tris-HCl. Incubation of the enzyme in Tris-maleate for one h with high concentrations of dithiotreitol restores the positive homotropic interactions. 3. It is proposed, that the abnormalities of the pyruvate kinase of some patients with acquired pyruvate kinase deficiency, obtained from a study in Tris-maleate, may partly be induced by the buffer itself."}
{"id": "PMID:476962", "title": "Fatty acid differences amongst leukemia cell types, with special reference to hairy cell leukemia: a preliminary report.", "content": "Fatty acid analyses of malignant cells from hairy cell leukemia patients revealed a profile which was rich in arachidonate and relatively low in linoleate and oleate. This profile was distinct from that of mononuclear cells derived from patients with other lymphoproliferative diseases or myeloproliferative disorders, and may reflect the unique membrane morphology of the hairy cell.", "contents": "Fatty acid differences amongst leukemia cell types, with special reference to hairy cell leukemia: a preliminary report. Fatty acid analyses of malignant cells from hairy cell leukemia patients revealed a profile which was rich in arachidonate and relatively low in linoleate and oleate. This profile was distinct from that of mononuclear cells derived from patients with other lymphoproliferative diseases or myeloproliferative disorders, and may reflect the unique membrane morphology of the hairy cell."}
{"id": "PMID:476964", "title": "A sensitive fluorescent assay for N-acetyltransferase activity in human lymphocytes from newborns and adults.", "content": "We have developed a simplified assay for the enzyme N-acetyltransferase, based upon the loss of fluorescence after acetylation of the substrate p-aminobenzoic acid. This method is sufficiently sensitive to permit the quantitation of N-acetyltransferase activity in 10(5) human lymphocytes. Using this method, we have compared the level of N-acetyltransferase activity in lymphocytes from adult peripheral blood and from cord blood samples.", "contents": "A sensitive fluorescent assay for N-acetyltransferase activity in human lymphocytes from newborns and adults. We have developed a simplified assay for the enzyme N-acetyltransferase, based upon the loss of fluorescence after acetylation of the substrate p-aminobenzoic acid. This method is sufficiently sensitive to permit the quantitation of N-acetyltransferase activity in 10(5) human lymphocytes. Using this method, we have compared the level of N-acetyltransferase activity in lymphocytes from adult peripheral blood and from cord blood samples."}
{"id": "PMID:476963", "title": "Aggregation-induced stability of dexamethasone receptors in rat kidney.", "content": "The stability of the dexamethasone-receptor complex of rat kidney cytosol was studied using homogenizing media of varying pH and composition. It was found that the complex is less stable with buffers usually employed for the study of steroid receptors such as Tris, EDTA etc., whereas the stability increases considerably with a solution composed of monothioglycerol 12 mmol/l and glycerol 5% in distilled water. Sepharose 4-B column chromatography revealed that the increased stability was associated with a larger form (probably an aggregate) of the receptor-steroid complex.", "contents": "Aggregation-induced stability of dexamethasone receptors in rat kidney. The stability of the dexamethasone-receptor complex of rat kidney cytosol was studied using homogenizing media of varying pH and composition. It was found that the complex is less stable with buffers usually employed for the study of steroid receptors such as Tris, EDTA etc., whereas the stability increases considerably with a solution composed of monothioglycerol 12 mmol/l and glycerol 5% in distilled water. Sepharose 4-B column chromatography revealed that the increased stability was associated with a larger form (probably an aggregate) of the receptor-steroid complex."}
{"id": "PMID:476965", "title": "Pacemakers, cardiac mapping and EEG in the community hospital: a concise review of background and basic technical considerations.", "content": "The heart is a pump. The objective of cardiac pacing is to improve the cardiac output. The most popular method of cardiac pacing (RV) is not physiologic and results in reduced stroke output when compared to that of normal sinus or atrial paced rhythm. Recognition of pacemaker induced low output states and pacemaker aggravated neurological symptoms has quickened interest in physiologic atrial pacing. The increased complexity of atrial pacing has been discouraging in the past, but technical advances in electrode and pacemaker design are promising. Selection of the optimal pacing site appears critical and requires the determination of the focal electrical potential and stimulating threshold before electrodes are positioned. With minimal expense, the EEG can be adapted for this type of cardiac mapping in the community hospital.", "contents": "Pacemakers, cardiac mapping and EEG in the community hospital: a concise review of background and basic technical considerations. The heart is a pump. The objective of cardiac pacing is to improve the cardiac output. The most popular method of cardiac pacing (RV) is not physiologic and results in reduced stroke output when compared to that of normal sinus or atrial paced rhythm. Recognition of pacemaker induced low output states and pacemaker aggravated neurological symptoms has quickened interest in physiologic atrial pacing. The increased complexity of atrial pacing has been discouraging in the past, but technical advances in electrode and pacemaker design are promising. Selection of the optimal pacing site appears critical and requires the determination of the focal electrical potential and stimulating threshold before electrodes are positioned. With minimal expense, the EEG can be adapted for this type of cardiac mapping in the community hospital."}
{"id": "PMID:476967", "title": "Acute subdural hematoma and the periodic lateralized epileptiform discharges.", "content": "Focal motor seizures associated with periodic lateralized epileptiform discharges (PLEDs) were observed in two patients with acute subudral hematoma. Following the surgery, one patient continued to exhibit PLEDs but clinical seizures were absent PLEDs recurred in the second patient due to inadequate anticonvulsant medication. Autopsy study in one patient suggests that underlying cerebral contusion was not responsible for the occurrence of PLEDs.", "contents": "Acute subdural hematoma and the periodic lateralized epileptiform discharges. Focal motor seizures associated with periodic lateralized epileptiform discharges (PLEDs) were observed in two patients with acute subudral hematoma. Following the surgery, one patient continued to exhibit PLEDs but clinical seizures were absent PLEDs recurred in the second patient due to inadequate anticonvulsant medication. Autopsy study in one patient suggests that underlying cerebral contusion was not responsible for the occurrence of PLEDs."}
{"id": "PMID:476968", "title": "Visual evoked potentials in Reye's syndrome.", "content": "Visual evoked potentials (VEPs) induced by flash stimulation in a child who was recovering from Reye's syndrome with complaints of poor left-eye vision were recorded. Ophthalmological examination disclosed intact visual fields and normal visual acuity. Analysis of VEP's, however, showed a grossly abnormal configuration of wave forms, marked prolongation in latency and reduction in amplitude when the left eye was stimulated. Follow-up observation 3 weeks later showed parallel improvements in VEPs and subjective complaints. Thus, from VEP analysis, it may be possible to detect subtle changes in the visual system that have diagnostic and prognostic value.", "contents": "Visual evoked potentials in Reye's syndrome. Visual evoked potentials (VEPs) induced by flash stimulation in a child who was recovering from Reye's syndrome with complaints of poor left-eye vision were recorded. Ophthalmological examination disclosed intact visual fields and normal visual acuity. Analysis of VEP's, however, showed a grossly abnormal configuration of wave forms, marked prolongation in latency and reduction in amplitude when the left eye was stimulated. Follow-up observation 3 weeks later showed parallel improvements in VEPs and subjective complaints. Thus, from VEP analysis, it may be possible to detect subtle changes in the visual system that have diagnostic and prognostic value."}
{"id": "PMID:476969", "title": "Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in men.", "content": "Unconjugated oestrone and unconjugated oestradiol-17 beta were measured in 24 h urine specimens from twenty normal men. The concentrations of non-protein-bound oestrone and non-protein-bound oestradiol-17 beta, in samples of blood obtained from these subjects during the time in which the urine was collected, were measured by equilibrium dialysis (using a correction for plasma dilution). The mean excretion of unconjugated oestrone (0.44+/- 0.36 nmol/24 h, mean +/- SD) was significantly greater (Pless than 0.01) than that of unconjugated oestradiol-17 beta (0.20 +/- 0.13 nmol/24 h). The concentrations of non-protein-bound oestrone and non-protein-bound oestradiol-17 beta in plasma were 5.52 +/- 2.69pmol/1 and 2.42 +/- 0.73 pmol/1, respectively. There was no correlation between the quantity of unconjugated oestrone excreted and the concentration of non-protein-bound oestrone in plasma (r=0.27) nor between the quantity of unconjugated oestradiol-17 beta in the urine and the concentration of non-protein-bound oestradiol-17 beta in plasma (r=0.05). Therefore in normal men, estimation of unconjugated oestrone or oestradiol-17 beta in urine provides no guide to the concentration of the corresponding non-protein-bound oestrogen in plasma.", "contents": "Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in men. Unconjugated oestrone and unconjugated oestradiol-17 beta were measured in 24 h urine specimens from twenty normal men. The concentrations of non-protein-bound oestrone and non-protein-bound oestradiol-17 beta, in samples of blood obtained from these subjects during the time in which the urine was collected, were measured by equilibrium dialysis (using a correction for plasma dilution). The mean excretion of unconjugated oestrone (0.44+/- 0.36 nmol/24 h, mean +/- SD) was significantly greater (Pless than 0.01) than that of unconjugated oestradiol-17 beta (0.20 +/- 0.13 nmol/24 h). The concentrations of non-protein-bound oestrone and non-protein-bound oestradiol-17 beta in plasma were 5.52 +/- 2.69pmol/1 and 2.42 +/- 0.73 pmol/1, respectively. There was no correlation between the quantity of unconjugated oestrone excreted and the concentration of non-protein-bound oestrone in plasma (r=0.27) nor between the quantity of unconjugated oestradiol-17 beta in the urine and the concentration of non-protein-bound oestradiol-17 beta in plasma (r=0.05). Therefore in normal men, estimation of unconjugated oestrone or oestradiol-17 beta in urine provides no guide to the concentration of the corresponding non-protein-bound oestrogen in plasma."}
{"id": "PMID:476970", "title": "Amniotic fluid concentrations of 3,3',5'-tri-iodothyronine (reverse T3), 3,3'-di-iodothyronine, 3,5,3'-tri-iodothyronine (T3) and thyroxine (T4) in normal and complicated pregnancy.", "content": "Amniotic fluid concentrations of 3,3',5'-tri-iodothyronine (rT3), 3,3'-Di-iodothyronine (3,3'-T2), 3,5,3'-tri-iodothyronine (T3) and T4 were studied in 384 women during normal and complicated pregnancy. An inverse correlation was observed between decreasing rT3 and increasing 3,3'-T2 concentrations in amniotic fluid with gestational age. The mean rT3 level in normal pregnancy was 2.81 nmol/1 at 12-20 weeks and decreased significantly to 1.06 nmol/1 at 36-42 weeks of gestation. The mean 3,3'-T2 concentration was 49.1 pmol/1 at12-20 weeks increasing to 119 pmol/1 at 36-42 weeks. The mean T4 value of 3.83 nmol/1 at 12-20 weeks was about half that of later periods. The T3 concentration in a random sample of 45 amniotic fluids ranged from less than 28 to 370 pmol/1 (mean 102 pmol/1). The mean rT3, 3,3'-T2 and T4 values measured in patients with intra-uterine malnutrition, gestation diabetes, tocolysis, placental insufficiency and rhesus incompatibility at 31-40 weeks of gestation were not significantly different from those in uncomplicated pregnancy. Significantly decreased rT3 and T4 concentrations were found in toxaemia. From the results obtained in complicated pregnancy it may be concluded that measurements of iodothyronines, especially rT3, in amniotic fluid have insignificant diagnostic value in the recognition of intra-uterine lesions with the probable exception of fetal hypothyroidism. The analysis of the dependence of iodothyronine concentrations on the gestational age showed a maximum of rT3 and T4 levels between 20 and 30 weeks of pregnancy. This marked rise of iodothyronine concentrations in amniotic fluid at mid-gestation may be due to the onsetting maturation of the hypothalamic-pituitary-thyroid control system of the fetus.", "contents": "Amniotic fluid concentrations of 3,3',5'-tri-iodothyronine (reverse T3), 3,3'-di-iodothyronine, 3,5,3'-tri-iodothyronine (T3) and thyroxine (T4) in normal and complicated pregnancy. Amniotic fluid concentrations of 3,3',5'-tri-iodothyronine (rT3), 3,3'-Di-iodothyronine (3,3'-T2), 3,5,3'-tri-iodothyronine (T3) and T4 were studied in 384 women during normal and complicated pregnancy. An inverse correlation was observed between decreasing rT3 and increasing 3,3'-T2 concentrations in amniotic fluid with gestational age. The mean rT3 level in normal pregnancy was 2.81 nmol/1 at 12-20 weeks and decreased significantly to 1.06 nmol/1 at 36-42 weeks of gestation. The mean 3,3'-T2 concentration was 49.1 pmol/1 at12-20 weeks increasing to 119 pmol/1 at 36-42 weeks. The mean T4 value of 3.83 nmol/1 at 12-20 weeks was about half that of later periods. The T3 concentration in a random sample of 45 amniotic fluids ranged from less than 28 to 370 pmol/1 (mean 102 pmol/1). The mean rT3, 3,3'-T2 and T4 values measured in patients with intra-uterine malnutrition, gestation diabetes, tocolysis, placental insufficiency and rhesus incompatibility at 31-40 weeks of gestation were not significantly different from those in uncomplicated pregnancy. Significantly decreased rT3 and T4 concentrations were found in toxaemia. From the results obtained in complicated pregnancy it may be concluded that measurements of iodothyronines, especially rT3, in amniotic fluid have insignificant diagnostic value in the recognition of intra-uterine lesions with the probable exception of fetal hypothyroidism. The analysis of the dependence of iodothyronine concentrations on the gestational age showed a maximum of rT3 and T4 levels between 20 and 30 weeks of pregnancy. This marked rise of iodothyronine concentrations in amniotic fluid at mid-gestation may be due to the onsetting maturation of the hypothalamic-pituitary-thyroid control system of the fetus."}
{"id": "PMID:476972", "title": "Decreased blood pressure in response to an angiotensin II antagonist in Addison's disease.", "content": "The synthetic angiotensin II antagonist, 1-sarcosine, 8-isoleucine angiotensin II was infused in two patients with untreated Addison's disease. Blood pressure decreased following infusion of this angiotensin II antagonist. Re-infusion of the antagonist after cortisol replacement therapy for one month caused a slight increase in blood pressure. Addison's disease is one of the conditions in which the renin-angiotensin system is involved in the maintenance of blood pressure.", "contents": "Decreased blood pressure in response to an angiotensin II antagonist in Addison's disease. The synthetic angiotensin II antagonist, 1-sarcosine, 8-isoleucine angiotensin II was infused in two patients with untreated Addison's disease. Blood pressure decreased following infusion of this angiotensin II antagonist. Re-infusion of the antagonist after cortisol replacement therapy for one month caused a slight increase in blood pressure. Addison's disease is one of the conditions in which the renin-angiotensin system is involved in the maintenance of blood pressure."}
{"id": "PMID:476973", "title": "Prolactin concentrations in normal menstrual cycles and conception cycles.", "content": "Plasma prolactin concentrations were measured daily throughout twenty-three menstrual cycles from regularly ovulating women. In five of the cycles conception occurred spontaneously. The frequency distribution of prolactin concentrations was calculated and an appropriate linear transform obtained. Means and 95% confidence limits were determined by inspection of the logarithmic probability plots and these gave mean concentrations (and ranges) of 302 (106-871) mU/1 for the nonconception cycles and 178 (58-550) mU/1 for the conception cycles. Distribution of prolactin concentrations was log-normal in all cases, although the population containing the conception cycles was distinct from the population of non-conception cycles. It is suggested that the normal range found in ovulating women is 80-800 mU/1 but that for conception to occur levels should be slightly lower and approximate to the range 60-600 mU/1. Thus the lower limit of normal is about 60 mU/1 and the upper limit about 800 mU/1. Any concentration over 800 mU/1 should be designated as hyperprolactinaemia.", "contents": "Prolactin concentrations in normal menstrual cycles and conception cycles. Plasma prolactin concentrations were measured daily throughout twenty-three menstrual cycles from regularly ovulating women. In five of the cycles conception occurred spontaneously. The frequency distribution of prolactin concentrations was calculated and an appropriate linear transform obtained. Means and 95% confidence limits were determined by inspection of the logarithmic probability plots and these gave mean concentrations (and ranges) of 302 (106-871) mU/1 for the nonconception cycles and 178 (58-550) mU/1 for the conception cycles. Distribution of prolactin concentrations was log-normal in all cases, although the population containing the conception cycles was distinct from the population of non-conception cycles. It is suggested that the normal range found in ovulating women is 80-800 mU/1 but that for conception to occur levels should be slightly lower and approximate to the range 60-600 mU/1. Thus the lower limit of normal is about 60 mU/1 and the upper limit about 800 mU/1. Any concentration over 800 mU/1 should be designated as hyperprolactinaemia."}
{"id": "PMID:476974", "title": "Effect of human growth hormone therapy on head circumference in children with hypopituitarism.", "content": "Head circumference was measured before and during hGH therapy in fourteen children with isolated growth hormone deficiency (IGHD) and in twenty-one children with multiple pituitary hormone deficiencies (MPHD). In both groups there was a retardation in growth of the neurocranium, more marked in the children with IGHD, which was less than the retardation in linear height. In the group with IGHD, initiation of hGH therapy before a chronological age of 5 and a bone age of 3 had been reached led to a rapid catch-up in cranial growth with normalization of the head size. In older children the induction of head growth was similar to that achieved by the long bones but without a true catch-up phenomenon. In three adults with hereditary IGHD without therapy or with irregular treatment during late puberty, the head circumference was below normal range. In view of the possible role played by development of brain tissue upon cranial size, the importance of early diagnosis and initiation of therapy in infants and young children with a deficiency of hGH is stressed.", "contents": "Effect of human growth hormone therapy on head circumference in children with hypopituitarism. Head circumference was measured before and during hGH therapy in fourteen children with isolated growth hormone deficiency (IGHD) and in twenty-one children with multiple pituitary hormone deficiencies (MPHD). In both groups there was a retardation in growth of the neurocranium, more marked in the children with IGHD, which was less than the retardation in linear height. In the group with IGHD, initiation of hGH therapy before a chronological age of 5 and a bone age of 3 had been reached led to a rapid catch-up in cranial growth with normalization of the head size. In older children the induction of head growth was similar to that achieved by the long bones but without a true catch-up phenomenon. In three adults with hereditary IGHD without therapy or with irregular treatment during late puberty, the head circumference was below normal range. In view of the possible role played by development of brain tissue upon cranial size, the importance of early diagnosis and initiation of therapy in infants and young children with a deficiency of hGH is stressed."}
{"id": "PMID:476976", "title": "Serum T3 and T4 levels in patients with anorexia nervosa showing transient hyperthyroidism during weight gain.", "content": "Serum thyroxine (T4) and triiodothyronine (T3) levels were measured in a group of thirty-three patients with anorexia nervosa (AN) and compared with twenty-five control women presenting with hirsutism and twenty-one patients with primary myxoedema. T3 levels in the AN patients were significantly lower than in the control subjects and in the patients with myxoedema while T4 levels were significantly higher than in the patients with hypothyroidism but significantly lower than in the control group. Seventeen anorexia patients had further T3 and T4 levels measured following an arbitrary 25% weight gain and both levels had increased significantly. For individual patients, the absolute rise in T3 levels was significantly correlated with the rate of weight gain. Thirteen patients had serial T3 and T4 levels measured during their periods of weight gain. Eight of these subjects showed a gradual rise in T3 levels from subnormal or low normal levels to values in the upper normal range. Four subjects showed a distinct and self limiting overshoot of T3 levels and, associated with this, the patients had clinical features of mild hyperthyroidism.", "contents": "Serum T3 and T4 levels in patients with anorexia nervosa showing transient hyperthyroidism during weight gain. Serum thyroxine (T4) and triiodothyronine (T3) levels were measured in a group of thirty-three patients with anorexia nervosa (AN) and compared with twenty-five control women presenting with hirsutism and twenty-one patients with primary myxoedema. T3 levels in the AN patients were significantly lower than in the control subjects and in the patients with myxoedema while T4 levels were significantly higher than in the patients with hypothyroidism but significantly lower than in the control group. Seventeen anorexia patients had further T3 and T4 levels measured following an arbitrary 25% weight gain and both levels had increased significantly. For individual patients, the absolute rise in T3 levels was significantly correlated with the rate of weight gain. Thirteen patients had serial T3 and T4 levels measured during their periods of weight gain. Eight of these subjects showed a gradual rise in T3 levels from subnormal or low normal levels to values in the upper normal range. Four subjects showed a distinct and self limiting overshoot of T3 levels and, associated with this, the patients had clinical features of mild hyperthyroidism."}
{"id": "PMID:476975", "title": "Disturbed mineral metabolism in hyperthyroidism: good correlation with tri-iodothyronine.", "content": "Mineral metabolism is frequently disturbed in hyperthyroidism. In a group of seventy-two patients with hyperthyroidism, we observed an increase in serum diffusible calcium in 50% of the cases, elevated inorganic phosphorus in 30% and elevated alkaline phosphatase in 44% of the cases. Correlations existed between the values of diffusible calcium, inorganic phosphorus, alkaline phosphatase and certain indices of thyroid function (T4, FT41, T3, FT3I), of which that with T3 was the best (P less than 0.001). Our results suggest that the magnitude of the disturbances of mineral metabolism depends on the severity of the hyperthyroidism and that it is the T3 level that constitutes the best index of that severity.", "contents": "Disturbed mineral metabolism in hyperthyroidism: good correlation with tri-iodothyronine. Mineral metabolism is frequently disturbed in hyperthyroidism. In a group of seventy-two patients with hyperthyroidism, we observed an increase in serum diffusible calcium in 50% of the cases, elevated inorganic phosphorus in 30% and elevated alkaline phosphatase in 44% of the cases. Correlations existed between the values of diffusible calcium, inorganic phosphorus, alkaline phosphatase and certain indices of thyroid function (T4, FT41, T3, FT3I), of which that with T3 was the best (P less than 0.001). Our results suggest that the magnitude of the disturbances of mineral metabolism depends on the severity of the hyperthyroidism and that it is the T3 level that constitutes the best index of that severity."}
{"id": "PMID:476978", "title": "Plasma levels of somatostatin-like immunoreactivity-independence of kidney function.", "content": "Plasma levels of somatostatin-like immunoreactivity (SLI) and gastrin were determined by radioimmunoassay in patients with varying degrees of renal dysfunction. The plasma SLI was not increased in patients with end-stage renal failure as compared to healthy controls. In patients with varying degrees of renal insufficiency no significant correlation was found between the plasma SLI and kidney function as estimated by the 51Cr-EDTA-clearance rate. In uraemic patients a significant inverse correlation was found between plasma SLI and plasma gastrin.", "contents": "Plasma levels of somatostatin-like immunoreactivity-independence of kidney function. Plasma levels of somatostatin-like immunoreactivity (SLI) and gastrin were determined by radioimmunoassay in patients with varying degrees of renal dysfunction. The plasma SLI was not increased in patients with end-stage renal failure as compared to healthy controls. In patients with varying degrees of renal insufficiency no significant correlation was found between the plasma SLI and kidney function as estimated by the 51Cr-EDTA-clearance rate. In uraemic patients a significant inverse correlation was found between plasma SLI and plasma gastrin."}
{"id": "PMID:476979", "title": "Ectopic functioning adrenocortico-myelolipoma in longstanding Nelson's syndrome.", "content": "A 26-year-old man with the Nelson's syndrome is described, in whom an abdominal tumour developed, accompanied by recurrent Cushing's syndrome 11 years after total adrenalectomy. Upon removal the tumour was a myelolipoma containing islands of adrenocortical-like elements. On incubation of the tumour with radioactive precursors, its capacity to produce cortisol in vitro was shown. The name adrenocortico-myelolipoma is suggested for this tumour.", "contents": "Ectopic functioning adrenocortico-myelolipoma in longstanding Nelson's syndrome. A 26-year-old man with the Nelson's syndrome is described, in whom an abdominal tumour developed, accompanied by recurrent Cushing's syndrome 11 years after total adrenalectomy. Upon removal the tumour was a myelolipoma containing islands of adrenocortical-like elements. On incubation of the tumour with radioactive precursors, its capacity to produce cortisol in vitro was shown. The name adrenocortico-myelolipoma is suggested for this tumour."}
{"id": "PMID:476977", "title": "The effect of somatostatin on metabolic and hormonal changes during and after exercise.", "content": "The effect of intravenous somatostatin on blood levels of metabolites and hormones has been examined in normal subjects who performed a 30-minute period of bicycle exercises at 70% maximal exercise capacity. The results have been compared with control studies in the same subjects. Measurements were made of blood levels of lactate, glucose, free fatty acids, glycerol, acetoacetate, 3-hydroxybutyrate, insulin, glucagon, growth hormone (hGH) and prolactin. Growth hormone and glucagon release were suppressed during exercise with somatostatin and there was a subsequent elevation during recovery. There was slight post-exercise depression of insulin, but no alteration of plasma prolactin secretion. Blood glucose was reduced during exercise with somatostatin and increased during recovery. The elevation of ketone bodies after exercise was greater in the investigation with somatostatin, but there were no significant changes in other metabolites. Somatostatin, although causing inhibition of hGH release, appeared to have no significant effect upon fatty acid mobilization during exercise.", "contents": "The effect of somatostatin on metabolic and hormonal changes during and after exercise. The effect of intravenous somatostatin on blood levels of metabolites and hormones has been examined in normal subjects who performed a 30-minute period of bicycle exercises at 70% maximal exercise capacity. The results have been compared with control studies in the same subjects. Measurements were made of blood levels of lactate, glucose, free fatty acids, glycerol, acetoacetate, 3-hydroxybutyrate, insulin, glucagon, growth hormone (hGH) and prolactin. Growth hormone and glucagon release were suppressed during exercise with somatostatin and there was a subsequent elevation during recovery. There was slight post-exercise depression of insulin, but no alteration of plasma prolactin secretion. Blood glucose was reduced during exercise with somatostatin and increased during recovery. The elevation of ketone bodies after exercise was greater in the investigation with somatostatin, but there were no significant changes in other metabolites. Somatostatin, although causing inhibition of hGH release, appeared to have no significant effect upon fatty acid mobilization during exercise."}
{"id": "PMID:476980", "title": "Mineralocorticoid replacement in Addison's disease.", "content": "Eight patients with treated Addison's disease were studied whilst receiving different doses of fludrocortisone together with a constant intake of glucocorticoid. Plasma renin activity (PRA), blood pressure, pulse rate and plasma potassium and urea concentrations were measured after 2-week periods on each dose. In two patients, PRA measurements indicated that mineralocorticoid replacement therapy had been inadequate. In four others, PRA remained normal throughout the study, even after fludrocortisone had been discontinued, suggesting that the drug was unnecessary for the maintenance of normal sodium balance in these patients. Asymptomatic fludrocortisone overdosage was indicated by a low plasma potassium concentration, but not by PRA measurements which failed in this study to distinguish between adequate and excessive mineralocorticoid replacement.", "contents": "Mineralocorticoid replacement in Addison's disease. Eight patients with treated Addison's disease were studied whilst receiving different doses of fludrocortisone together with a constant intake of glucocorticoid. Plasma renin activity (PRA), blood pressure, pulse rate and plasma potassium and urea concentrations were measured after 2-week periods on each dose. In two patients, PRA measurements indicated that mineralocorticoid replacement therapy had been inadequate. In four others, PRA remained normal throughout the study, even after fludrocortisone had been discontinued, suggesting that the drug was unnecessary for the maintenance of normal sodium balance in these patients. Asymptomatic fludrocortisone overdosage was indicated by a low plasma potassium concentration, but not by PRA measurements which failed in this study to distinguish between adequate and excessive mineralocorticoid replacement."}
{"id": "PMID:476982", "title": "Propranolol, triiodothyronine, reverse triiodothyronine and thyroid disease.", "content": "Propranolol alone was given to sixteen hyperthyroid, and concomitantly with thyroxine therapy to ten hypothyroid patients. Following treatment of the hyperthyroid group for 1-2 weeks there was a significant decrease in serum triiodothyronine (T3) which correlated with the plasma propranolol steady state concentration. The serum reverse T3 (rT3) rose significantly. Weight loss ceased in this group while weight gain occurred in patients who had a marked fall in serum T3. One patient with T3 toxicosis went into remission. The reduction in serum T3 was maintained in six patients receiving propranolol for more than 1 month. In the hypothyroid group the mean serum T3 level achieved with 0.15 mg thyroxine per day was significantly lower than in a control group who did not receive propranolol. In five patients following propranolol withdrawal there was a significant rise in T3, a fall in rT3 and TSH, and weight loss. Propranol may therefore have a clinically significant and direct action on the peripheral conversion of thyroxine to T3 and rT3.", "contents": "Propranolol, triiodothyronine, reverse triiodothyronine and thyroid disease. Propranolol alone was given to sixteen hyperthyroid, and concomitantly with thyroxine therapy to ten hypothyroid patients. Following treatment of the hyperthyroid group for 1-2 weeks there was a significant decrease in serum triiodothyronine (T3) which correlated with the plasma propranolol steady state concentration. The serum reverse T3 (rT3) rose significantly. Weight loss ceased in this group while weight gain occurred in patients who had a marked fall in serum T3. One patient with T3 toxicosis went into remission. The reduction in serum T3 was maintained in six patients receiving propranolol for more than 1 month. In the hypothyroid group the mean serum T3 level achieved with 0.15 mg thyroxine per day was significantly lower than in a control group who did not receive propranolol. In five patients following propranolol withdrawal there was a significant rise in T3, a fall in rT3 and TSH, and weight loss. Propranol may therefore have a clinically significant and direct action on the peripheral conversion of thyroxine to T3 and rT3."}
{"id": "PMID:476983", "title": "Screening for neonatal hypothyroidism by thyroxine and thyrotrophin radioimmunoassays using dried blood samples on filter paper.", "content": "A routine and automatized methodology for thyroxine (T4) and thyrotrophin (TSH) radioimmunoassay (RIA) using dried blood samples on filter paper is described. T4-RIA was performed on one single dot (5 mm diameter equivalent to 4 microliters of serum) while two dots were necessary for TSH-RIA. Reference filter papers were introduced in each assay for quality control. In a preliminary study on 4,155 neonates, samples generally obtained between the 5th--7th day gave a mean 'dot-T4' of 97.95 +/- 36.04 nmol/l and a mean 'dot-TSH' of 10.19 mU/l +/- 8.25, corresponding to 2.47 mU/l of serum. Within an 18-month period (November 1976-April 1978), a total of 16.522 neonates have been screened allowing detection of three cases of congenital hypothyroidism (incidence 1 : 5507), two cases of congenitally low TBG and thirty-three cases of transient hypothyroidism.", "contents": "Screening for neonatal hypothyroidism by thyroxine and thyrotrophin radioimmunoassays using dried blood samples on filter paper. A routine and automatized methodology for thyroxine (T4) and thyrotrophin (TSH) radioimmunoassay (RIA) using dried blood samples on filter paper is described. T4-RIA was performed on one single dot (5 mm diameter equivalent to 4 microliters of serum) while two dots were necessary for TSH-RIA. Reference filter papers were introduced in each assay for quality control. In a preliminary study on 4,155 neonates, samples generally obtained between the 5th--7th day gave a mean 'dot-T4' of 97.95 +/- 36.04 nmol/l and a mean 'dot-TSH' of 10.19 mU/l +/- 8.25, corresponding to 2.47 mU/l of serum. Within an 18-month period (November 1976-April 1978), a total of 16.522 neonates have been screened allowing detection of three cases of congenital hypothyroidism (incidence 1 : 5507), two cases of congenitally low TBG and thirty-three cases of transient hypothyroidism."}
{"id": "PMID:476984", "title": "Effect of somatostatin on abnormal growth hormone and prolactin secretion in patients with the carcinoid syndrome.", "content": "Growth hormone (GH) secretion has been studied in two patients with the carcinoid syndrome during glucose loading and growth hormone-release inhibiting hormone (GHRIH, somatostatin) infusion. Both patients had elevated fasting GH levels which were not suppressed by glucose; GH levels fell rapidly during GHRIH infusion. One patient also had hyperprolactinaemia with galactorrhoea and the prolactin (PRL) levels were unaltered by GHRIH. The association between carcinoid tumours and abnormalities of GH and PRL secretion is discussed.", "contents": "Effect of somatostatin on abnormal growth hormone and prolactin secretion in patients with the carcinoid syndrome. Growth hormone (GH) secretion has been studied in two patients with the carcinoid syndrome during glucose loading and growth hormone-release inhibiting hormone (GHRIH, somatostatin) infusion. Both patients had elevated fasting GH levels which were not suppressed by glucose; GH levels fell rapidly during GHRIH infusion. One patient also had hyperprolactinaemia with galactorrhoea and the prolactin (PRL) levels were unaltered by GHRIH. The association between carcinoid tumours and abnormalities of GH and PRL secretion is discussed."}
{"id": "PMID:476985", "title": "Free-running circadian plasma cortisol rhythm in a blind human subject.", "content": "The plasma cortisol rhythm in man has been presumed to be an endogenous circadian rhythm, synchronized by some external stimulus to an exact 24-h period. Sleep/wake and 'social activity' cycles have been considered as candidates for this synchronizer. Previous studies have suggested that the dark/light phase shifts associated with the sleep-wake cycle may be the external synchronizer, rather than the sleep/wake cycle itself. A totally blind, but otherwise normal subject was studied for a period of 50 days. Her hourly sleep/wake status and hourly integrated mean plasma cortisol concentrations were determined, and the data were subjected to non-parametric mathematical analysis. The subject was found to have a free-running rhythm in plasma cortisol with a period of approximately 24.5 h. Her sleep/wake rhythm, determined by similar analysis, had a period of exactly 24 h. In addition to the dominant 24.5-h cortisol rhythm, there appeared to be a minor 24-h cortisol rhythm with a peak that coincided approximately with the time of awakening. It was not possible to determine whether this sleep/wake-related peak represented a minor component of the circadian rhythm, synchronized by some stimulus associated with sleep/wake activity, or merely an acute response to awakening itself. Nevertheless, the two rhythms exhibited 'beating' behaviour, resulting in maximal peak cortisol concentrations when they were in synchrony and minimal peak concentrations when they were not. It is concluded that environment dark/light phase shifts are the dominant synchronizer of the circadian rhythm in plasma cortisol concentrations in man, as they are for a variety of circadian rhythms in other living things.", "contents": "Free-running circadian plasma cortisol rhythm in a blind human subject. The plasma cortisol rhythm in man has been presumed to be an endogenous circadian rhythm, synchronized by some external stimulus to an exact 24-h period. Sleep/wake and 'social activity' cycles have been considered as candidates for this synchronizer. Previous studies have suggested that the dark/light phase shifts associated with the sleep-wake cycle may be the external synchronizer, rather than the sleep/wake cycle itself. A totally blind, but otherwise normal subject was studied for a period of 50 days. Her hourly sleep/wake status and hourly integrated mean plasma cortisol concentrations were determined, and the data were subjected to non-parametric mathematical analysis. The subject was found to have a free-running rhythm in plasma cortisol with a period of approximately 24.5 h. Her sleep/wake rhythm, determined by similar analysis, had a period of exactly 24 h. In addition to the dominant 24.5-h cortisol rhythm, there appeared to be a minor 24-h cortisol rhythm with a peak that coincided approximately with the time of awakening. It was not possible to determine whether this sleep/wake-related peak represented a minor component of the circadian rhythm, synchronized by some stimulus associated with sleep/wake activity, or merely an acute response to awakening itself. Nevertheless, the two rhythms exhibited 'beating' behaviour, resulting in maximal peak cortisol concentrations when they were in synchrony and minimal peak concentrations when they were not. It is concluded that environment dark/light phase shifts are the dominant synchronizer of the circadian rhythm in plasma cortisol concentrations in man, as they are for a variety of circadian rhythms in other living things."}
{"id": "PMID:476986", "title": "Rapid conversion of carbimazole to methimazole in serum; evidence for an enzymatic mechanism.", "content": "Carbimazole (CBZ) is one of the major drugs currently used for the treatment of Graves' disease. It is a carbethoxy derivative of methimazole (MMI), originally developed in the hope of obtaining a longer acting drug than methimazole. In the present study we have demonstrated that carbimazole is rapidly converted to methimazole in vitro by serum from rats and humans, and we have obtained evidence that this conversion is enzymatic. Experiments with [35S] CBZ in rats showed that the drug is so rapidly transformed to MMI after i.v. injection (within 3 min) that very little of the unchanged drug would be expected to reach the thyroid gland. The antithyroid action of CBZ in rats, therefore, can be ascribed entirely to the MMI to which it is rapidly converted. Although no experiments were performed with human subjects in vivo, the very rapid conversion of CBZ to MMI by human serum in vitro suggests that the antithyroid action of CBZ in humans can also be attributed to MMI. The original expectation of a longer acting drug has, therefore, not been met by CBZ. On the basis of the studies reported here there appears to be no advantage in using CBZ in preference to MMI for the treatment of Graves' disease. Although the in vivo action of CBZ must be attributed to its rapid conversion to MMI, the drug does possess inherent antithyroid activity. This was shown in the present study by the finding that CBZ is as potent as MMI in blocking thyroid peroxidase-catalysed iodination of thyroglobulin.", "contents": "Rapid conversion of carbimazole to methimazole in serum; evidence for an enzymatic mechanism. Carbimazole (CBZ) is one of the major drugs currently used for the treatment of Graves' disease. It is a carbethoxy derivative of methimazole (MMI), originally developed in the hope of obtaining a longer acting drug than methimazole. In the present study we have demonstrated that carbimazole is rapidly converted to methimazole in vitro by serum from rats and humans, and we have obtained evidence that this conversion is enzymatic. Experiments with [35S] CBZ in rats showed that the drug is so rapidly transformed to MMI after i.v. injection (within 3 min) that very little of the unchanged drug would be expected to reach the thyroid gland. The antithyroid action of CBZ in rats, therefore, can be ascribed entirely to the MMI to which it is rapidly converted. Although no experiments were performed with human subjects in vivo, the very rapid conversion of CBZ to MMI by human serum in vitro suggests that the antithyroid action of CBZ in humans can also be attributed to MMI. The original expectation of a longer acting drug has, therefore, not been met by CBZ. On the basis of the studies reported here there appears to be no advantage in using CBZ in preference to MMI for the treatment of Graves' disease. Although the in vivo action of CBZ must be attributed to its rapid conversion to MMI, the drug does possess inherent antithyroid activity. This was shown in the present study by the finding that CBZ is as potent as MMI in blocking thyroid peroxidase-catalysed iodination of thyroglobulin."}
{"id": "PMID:476987", "title": "Human growth hormone (hGH) stimulation tests: the sequential exercise and L-dopa procedure.", "content": "Forty-eight normal volunteers, thirteen subjects with short stature without apparent cause and thirty-one patients with delayed growth clinically highly suggestive of growth hormone deficiency (GHD), with chronological ages of 11.4 +/- 0.4 years (mean +/- SEM), 14.0 +/- 0.7 years and 12.8 +/- 0.8 years; height age of 11.0 +/- 0.4 years, 8.9 +/- 0.7 years and 6.3 +/- 0.4 years and bone age of 10.7 +/- 0.7 years, 9.9 +/- 0.8 years and 7.5 +/- 0.7 years respectively, were tested with provocative tests of human growth hormone (hGH) release-insulin-induced hypoglycaemia, arginine infusion, L-DOPA, exercise and sequential exercise and L-DOPA--in order to identify growth hormone deficiency. In the 'normal' subjects (control plus short stature) the sequential exercise and L-DOPA test induced the greatest peak and integrated secretion rates of plasma hGH (16.5 +- 1.2 ng/ml and 679 +/- 70 ng/ml/120 min respectively) when compared to other tests. This combined stumulus was the only one to which all 'normal' subjects responded: a similar degree of responsiveness has not been earlier described for other hGH-stimuli. The responses observed with all stimuli were significantly lower (P less than 0.001) in GHD group compared to the 'normal subjects'. Emphasis was given to the sequential exercise and L-DOPA test as an innocuous, sensitive and simplified procedure in the evaluation of children with growth retardation.", "contents": "Human growth hormone (hGH) stimulation tests: the sequential exercise and L-dopa procedure. Forty-eight normal volunteers, thirteen subjects with short stature without apparent cause and thirty-one patients with delayed growth clinically highly suggestive of growth hormone deficiency (GHD), with chronological ages of 11.4 +/- 0.4 years (mean +/- SEM), 14.0 +/- 0.7 years and 12.8 +/- 0.8 years; height age of 11.0 +/- 0.4 years, 8.9 +/- 0.7 years and 6.3 +/- 0.4 years and bone age of 10.7 +/- 0.7 years, 9.9 +/- 0.8 years and 7.5 +/- 0.7 years respectively, were tested with provocative tests of human growth hormone (hGH) release-insulin-induced hypoglycaemia, arginine infusion, L-DOPA, exercise and sequential exercise and L-DOPA--in order to identify growth hormone deficiency. In the 'normal' subjects (control plus short stature) the sequential exercise and L-DOPA test induced the greatest peak and integrated secretion rates of plasma hGH (16.5 +- 1.2 ng/ml and 679 +/- 70 ng/ml/120 min respectively) when compared to other tests. This combined stumulus was the only one to which all 'normal' subjects responded: a similar degree of responsiveness has not been earlier described for other hGH-stimuli. The responses observed with all stimuli were significantly lower (P less than 0.001) in GHD group compared to the 'normal subjects'. Emphasis was given to the sequential exercise and L-DOPA test as an innocuous, sensitive and simplified procedure in the evaluation of children with growth retardation."}
{"id": "PMID:476989", "title": "Deliberate hypotension in ENT surgery.", "content": "The development of deliberate hypotension for ENT surgery is described. A hitherto unrecorded case of paraplegia complicating hypotensive anaesthesia is reported. The use of sodium nitroprusside as a hypotensive agent is assessed. Indications and contraindications to deliberate hypotension are reviewed. Possible neurological damage is considered.", "contents": "Deliberate hypotension in ENT surgery. The development of deliberate hypotension for ENT surgery is described. A hitherto unrecorded case of paraplegia complicating hypotensive anaesthesia is reported. The use of sodium nitroprusside as a hypotensive agent is assessed. Indications and contraindications to deliberate hypotension are reviewed. Possible neurological damage is considered."}
{"id": "PMID:476990", "title": "Otosclerosis and pregnancy: a study of the influence of pregnancy on the hearing threshold before and after stapedectomy.", "content": "In a retrospective study of 144 fertile women subjected to operation for otosclerosis by the method of Shea or House, we found a fall in the hearing threshold in the operated ear which seemed to be a little greater in the patients who became pregnant after the operation than in those who did not. However, the hearing loss was significantly greater in the non-operated ear in the patients who became pregnant after the operation than in those who did not.", "contents": "Otosclerosis and pregnancy: a study of the influence of pregnancy on the hearing threshold before and after stapedectomy. In a retrospective study of 144 fertile women subjected to operation for otosclerosis by the method of Shea or House, we found a fall in the hearing threshold in the operated ear which seemed to be a little greater in the patients who became pregnant after the operation than in those who did not. However, the hearing loss was significantly greater in the non-operated ear in the patients who became pregnant after the operation than in those who did not."}
{"id": "PMID:476991", "title": "The occurrence of secretory otitis media in allergic children.", "content": "One hundred and forty-four ears (72 patients), forming part of the computer controlled respiratory allergy group of the University of Turku, were analyzed with tympanometry and audiometry to diagnose secretory otitis media. Effusion  was found in 3 ears (2 patients) and a pathological tympanogram in a total of 15 ears (10 patients). The incidence of secretory otitis media was 3% and of pathological tympanograms 14%. This study supports the opinion that immediate allergy is not an aetiological factor in secretory otitis media.", "contents": "The occurrence of secretory otitis media in allergic children. One hundred and forty-four ears (72 patients), forming part of the computer controlled respiratory allergy group of the University of Turku, were analyzed with tympanometry and audiometry to diagnose secretory otitis media. Effusion  was found in 3 ears (2 patients) and a pathological tympanogram in a total of 15 ears (10 patients). The incidence of secretory otitis media was 3% and of pathological tympanograms 14%. This study supports the opinion that immediate allergy is not an aetiological factor in secretory otitis media."}
{"id": "PMID:476992", "title": "Perineural spread in squamous cell carcinomas of the head and neck: a clinicopathological study.", "content": "Perineural spread of tumour has been demonstrated in 24% of an unselected series of 70 patients with squamous carcinomas of the head and neck treated by surgery. Slightly more than half the patients had primary tumours arising within the buccal cavity. Clinical features suggesting perineural infiltration were found in about two thirds of the cases; the symptoms and signs were usually sensory and occurred early in the disease. The mandibular division of the trigeminal nerve was most commonly affected. Perineural spread was more likely to occur with large carcinomas, moderate or poorly differentiated, showing local invasion and lymph node metastases. The associated pathological changes are described and a high incidence of damage to nerve fibres is recorded. The practical implications of perineural spread of tumour are discussed with reference to indications for more radical surgery or for more conservative measures supplemented with other modes of treatment.", "contents": "Perineural spread in squamous cell carcinomas of the head and neck: a clinicopathological study. Perineural spread of tumour has been demonstrated in 24% of an unselected series of 70 patients with squamous carcinomas of the head and neck treated by surgery. Slightly more than half the patients had primary tumours arising within the buccal cavity. Clinical features suggesting perineural infiltration were found in about two thirds of the cases; the symptoms and signs were usually sensory and occurred early in the disease. The mandibular division of the trigeminal nerve was most commonly affected. Perineural spread was more likely to occur with large carcinomas, moderate or poorly differentiated, showing local invasion and lymph node metastases. The associated pathological changes are described and a high incidence of damage to nerve fibres is recorded. The practical implications of perineural spread of tumour are discussed with reference to indications for more radical surgery or for more conservative measures supplemented with other modes of treatment."}
{"id": "PMID:476993", "title": "The forehead flap: a modification in technique.", "content": "The use of the forehead flap for reconstruction within the oral cavity after tumour resection is discussed. A minor modification in technique is described. The sub-zygomatic route to the oral cavity is used and a piece of teflon or sialalastic sheet is sewn over the raw surfaces of the flap which lies within the tunnel in the cheek to facilitate retrieval of the pedicle.", "contents": "The forehead flap: a modification in technique. The use of the forehead flap for reconstruction within the oral cavity after tumour resection is discussed. A minor modification in technique is described. The sub-zygomatic route to the oral cavity is used and a piece of teflon or sialalastic sheet is sewn over the raw surfaces of the flap which lies within the tunnel in the cheek to facilitate retrieval of the pedicle."}
{"id": "PMID:477009", "title": "Hallervorden-Spatz disease.", "content": "We had the opportunity to study a family, five of whose members were affected by the Hallervorden-Spatz disease (three males and twin girls). The characteristics of the condition were analyzed and compared with those cases considered by other authors to be affected by the condition. Intrafamilial and interfamilial variations were analysed, and it was the latter that contributed most to the overall variation of the condition. It was clearly established from the reported cases and our family that this is an autosomal recessive condition (P greater than 0.23 +/- 0.08). It is suggested that the condition probably originated in Europe and that it is caused by an inborn error of metabolism related to neuromelanin and the dopaminergic system. The condition affects the muscular tone and voluntary movements progressively, making voluntary coordinated movements, and chewing and swallowing almost impossible, and in the last part of its development mental deterioration, emaciation, severe feeding difficulties and visual impairment are common clinical manifestations. The ages of both onset and death are distributed in a unimodal curve. The mean survival time after diagnosis was 11.18 +/- 7.8 years.", "contents": "Hallervorden-Spatz disease. We had the opportunity to study a family, five of whose members were affected by the Hallervorden-Spatz disease (three males and twin girls). The characteristics of the condition were analyzed and compared with those cases considered by other authors to be affected by the condition. Intrafamilial and interfamilial variations were analysed, and it was the latter that contributed most to the overall variation of the condition. It was clearly established from the reported cases and our family that this is an autosomal recessive condition (P greater than 0.23 +/- 0.08). It is suggested that the condition probably originated in Europe and that it is caused by an inborn error of metabolism related to neuromelanin and the dopaminergic system. The condition affects the muscular tone and voluntary movements progressively, making voluntary coordinated movements, and chewing and swallowing almost impossible, and in the last part of its development mental deterioration, emaciation, severe feeding difficulties and visual impairment are common clinical manifestations. The ages of both onset and death are distributed in a unimodal curve. The mean survival time after diagnosis was 11.18 +/- 7.8 years."}
{"id": "PMID:477011", "title": "Partial trisomy 12q associated with a familial translocation.", "content": "A case is described of a female infant, who was trisomic for the terminal bands of the long arm of chromosome 12. Congenital abnormalities were present. Cytogenetic analysis on the proband's parents revealed the father to have a rare, simple translocation involving chromosomes 12 and 18.", "contents": "Partial trisomy 12q associated with a familial translocation. A case is described of a female infant, who was trisomic for the terminal bands of the long arm of chromosome 12. Congenital abnormalities were present. Cytogenetic analysis on the proband's parents revealed the father to have a rare, simple translocation involving chromosomes 12 and 18."}
{"id": "PMID:477013", "title": "Counseling problems when twins are discovered at genetic amniocentesis.", "content": "With the increased use of routine ultrasonography at the time of genetic amniocentesis, twins are increasingly likely to be discovered at the time when the procedure is to be carried out. In the presence of twins the likelihood of finding an abnormality may be significantly increased, but at the same time problems such as discordance between the twins for the abnormality, or the inability to test both twins may occur. This altered situation may affect the parents' decision  as to whether or not to undertake amniocentesis, and the need for additional counseling prior to proceeding with the amniocentesis is stressed.", "contents": "Counseling problems when twins are discovered at genetic amniocentesis. With the increased use of routine ultrasonography at the time of genetic amniocentesis, twins are increasingly likely to be discovered at the time when the procedure is to be carried out. In the presence of twins the likelihood of finding an abnormality may be significantly increased, but at the same time problems such as discordance between the twins for the abnormality, or the inability to test both twins may occur. This altered situation may affect the parents' decision  as to whether or not to undertake amniocentesis, and the need for additional counseling prior to proceeding with the amniocentesis is stressed."}
{"id": "PMID:477012", "title": "Alcohol metabolizing enzymes: studies of isozymes in human biopsies and cultured fibroblasts.", "content": "Rapid and sensitive micromethods for the study of alcohol dehydrogenase and adehyde dehydrogenase isozymes in skin extracts, cultured fibroblasts and other organs are presented. Possibilities for the application of these techniques to the study of interindividual variations in response to alcohol are discussed. While fibroblasts cultured from a skin biopsy from one Japanese individual revealed a heterodimer (ADH2 2-1) of alcohol dehydrogenase, skin extract from another Japanese showed a homodimer (ADH2 2-2). Up to four isozyme sets for aldehyde dehydrogenase (ALDH) were detected in various human organs and at least three sets were found in skin and fibroblasts extracts. Our preliminary data on liver, stomach, and skin indicate that ALDH is polymorphic and several loci are concerned in the determination of these isozyme sets.", "contents": "Alcohol metabolizing enzymes: studies of isozymes in human biopsies and cultured fibroblasts. Rapid and sensitive micromethods for the study of alcohol dehydrogenase and adehyde dehydrogenase isozymes in skin extracts, cultured fibroblasts and other organs are presented. Possibilities for the application of these techniques to the study of interindividual variations in response to alcohol are discussed. While fibroblasts cultured from a skin biopsy from one Japanese individual revealed a heterodimer (ADH2 2-1) of alcohol dehydrogenase, skin extract from another Japanese showed a homodimer (ADH2 2-2). Up to four isozyme sets for aldehyde dehydrogenase (ALDH) were detected in various human organs and at least three sets were found in skin and fibroblasts extracts. Our preliminary data on liver, stomach, and skin indicate that ALDH is polymorphic and several loci are concerned in the determination of these isozyme sets."}
{"id": "PMID:477015", "title": "Distal trisomy 17q.", "content": "A 3-year-old, male patient with trisomy 17q231qter due to a paternal t(5;17)(p151;q231) is compared to three other patients reported in the literature who are trisomic for the same segment due to a familial t(17;21)(q23;q22). The features common to the four patients are: profound mental retardation; dwarfism; frontal bossing and temporal retraction; narrow squinty eyes; thin lips with overlapping of the lower lip by the upper lip; very low-set and abnormal ears; cleft palate; and hyperlaxity of the ligaments. It thus seems possible to delineate a new cytogenetic syndrome.", "contents": "Distal trisomy 17q. A 3-year-old, male patient with trisomy 17q231qter due to a paternal t(5;17)(p151;q231) is compared to three other patients reported in the literature who are trisomic for the same segment due to a familial t(17;21)(q23;q22). The features common to the four patients are: profound mental retardation; dwarfism; frontal bossing and temporal retraction; narrow squinty eyes; thin lips with overlapping of the lower lip by the upper lip; very low-set and abnormal ears; cleft palate; and hyperlaxity of the ligaments. It thus seems possible to delineate a new cytogenetic syndrome."}
{"id": "PMID:477019", "title": "Chromosomal variants in mentally retarded and normal men.", "content": "The possible phenotypic effect of chromosomal variants is as yet an unsolved problem. QM- and C-banded chromosomes of 100 male patients with idiopathic mental retardation were compared with chromosomes of 100 Royal Military College cadets, as controls. Increased size of 9qh seems to be a factor with possible negative effects. 9qh- was found to be more common in the control sample. Another variant found more often in the retarded subjects was 16qh-. Increased frequencies of Yq+ or small inversions in chromosomes 3 and 9 were not found in the retarded.", "contents": "Chromosomal variants in mentally retarded and normal men. The possible phenotypic effect of chromosomal variants is as yet an unsolved problem. QM- and C-banded chromosomes of 100 male patients with idiopathic mental retardation were compared with chromosomes of 100 Royal Military College cadets, as controls. Increased size of 9qh seems to be a factor with possible negative effects. 9qh- was found to be more common in the control sample. Another variant found more often in the retarded subjects was 16qh-. Increased frequencies of Yq+ or small inversions in chromosomes 3 and 9 were not found in the retarded."}
{"id": "PMID:477020", "title": "Catecholamine metabolism in familial amyloid polyneuropathy.", "content": "In order to evaluate the involvement of the peripheral autonomic nervous system in the pathogenesis of type 1 familial amyloid polyneuropathy, the urinary excretion rates of catecholamines and serum dopamine-beta-hydroxylase (DB/) activity were examined in 22 patients at various clinical stages. Changes in both indices were closely linked to the progression of the illness; urinary excretion rates of catecholamines were first decreased in patients suffering from moderate autonomic dysfunction, while serum DBH activity was significantly reduced only in patients with far advanced disease. These findings suggested that patients with advanced disease might be suffering from a chronic deficiency of catecholamines in the peripheral sympathetic nerves. Administration of L-dopa, however, failed to improve the clinical manifestations.", "contents": "Catecholamine metabolism in familial amyloid polyneuropathy. In order to evaluate the involvement of the peripheral autonomic nervous system in the pathogenesis of type 1 familial amyloid polyneuropathy, the urinary excretion rates of catecholamines and serum dopamine-beta-hydroxylase (DB/) activity were examined in 22 patients at various clinical stages. Changes in both indices were closely linked to the progression of the illness; urinary excretion rates of catecholamines were first decreased in patients suffering from moderate autonomic dysfunction, while serum DBH activity was significantly reduced only in patients with far advanced disease. These findings suggested that patients with advanced disease might be suffering from a chronic deficiency of catecholamines in the peripheral sympathetic nerves. Administration of L-dopa, however, failed to improve the clinical manifestations."}
{"id": "PMID:477022", "title": "Evolutionary conservation of large chromosomal segments reflected in mammalian gene maps.", "content": "Conservation of genetic linkage over long periods of time is exemplifted. Comparisons are made between chromosomal regions in different species as well as within two species, man and the house mouse. Homologous regions are defined and the phenomenon of differential silencing of genes is described. The importance of conservation of particular sequences of genes is discussed in relation to medical genetics, animal breeding, evolutionary theory and genetic regulation.", "contents": "Evolutionary conservation of large chromosomal segments reflected in mammalian gene maps. Conservation of genetic linkage over long periods of time is exemplifted. Comparisons are made between chromosomal regions in different species as well as within two species, man and the house mouse. Homologous regions are defined and the phenomenon of differential silencing of genes is described. The importance of conservation of particular sequences of genes is discussed in relation to medical genetics, animal breeding, evolutionary theory and genetic regulation."}
{"id": "PMID:477023", "title": "Anthropometry in the Cri du Chat syndrome.", "content": "Anthropometric and cranial X-ray measurements of 35 individuals with a 5p- karyotype showed a general growth retardation. Height, weight, circumference of the thorax, pelvic breadth, and the size of the skull, face, hands and feet were all subnormal. Only the inner canthal distance was moderately increased, especially in young individuals, but there was no true hypertelorism. The palate was not high-arched. Large and small terminal deletions produced much the same anthropometric features; and the proband's sex did not have a major influence. Age variations within parameters examined followed the developmental pattern of normal individuals. A certain phenotypical variation in the Cri du Chat syndrome may therefore be attributed to normal changes or to intrapersonal conditions.", "contents": "Anthropometry in the Cri du Chat syndrome. Anthropometric and cranial X-ray measurements of 35 individuals with a 5p- karyotype showed a general growth retardation. Height, weight, circumference of the thorax, pelvic breadth, and the size of the skull, face, hands and feet were all subnormal. Only the inner canthal distance was moderately increased, especially in young individuals, but there was no true hypertelorism. The palate was not high-arched. Large and small terminal deletions produced much the same anthropometric features; and the proband's sex did not have a major influence. Age variations within parameters examined followed the developmental pattern of normal individuals. A certain phenotypical variation in the Cri du Chat syndrome may therefore be attributed to normal changes or to intrapersonal conditions."}
{"id": "PMID:477024", "title": "Immunological features of juvenile onset diabetic patients correlated to HLA type.", "content": "Ninety-six juvenile onset type diabetics showed an increase in the frequency of HLA B8 and B15 and a decrease in frequency of HLA B7 antigens. Sixty-four maturity onset diabetics showed no disturbance in the frequency of these antigens. Fifty-four of the juvenile onset type diabetics, with an average duration of disease of 3.2 years were tested for the presence of islet cell antibodies (ICAs). Thirty-two per cent were positive, the incidence decreasing from 70% in those patients tested within 1 year of diagnosis to zero in those patients tested within 1 year of diagnosis to zero in those patients tested more than 5 years after diagnosis. No correlation was found between the incidence of ICAs and either cell-mediated immune reactions or HLA type. B15 positive patients were associated with cell-mediated immune reactions to pancreatic antigens and with the presence of other tissue autoantibodies. HLA phenotypes were not associated with environmental data. Diabetic siblings had identical HLA A-B haplotypes more often than could be expected to occur by chance.", "contents": "Immunological features of juvenile onset diabetic patients correlated to HLA type. Ninety-six juvenile onset type diabetics showed an increase in the frequency of HLA B8 and B15 and a decrease in frequency of HLA B7 antigens. Sixty-four maturity onset diabetics showed no disturbance in the frequency of these antigens. Fifty-four of the juvenile onset type diabetics, with an average duration of disease of 3.2 years were tested for the presence of islet cell antibodies (ICAs). Thirty-two per cent were positive, the incidence decreasing from 70% in those patients tested within 1 year of diagnosis to zero in those patients tested within 1 year of diagnosis to zero in those patients tested more than 5 years after diagnosis. No correlation was found between the incidence of ICAs and either cell-mediated immune reactions or HLA type. B15 positive patients were associated with cell-mediated immune reactions to pancreatic antigens and with the presence of other tissue autoantibodies. HLA phenotypes were not associated with environmental data. Diabetic siblings had identical HLA A-B haplotypes more often than could be expected to occur by chance."}
{"id": "PMID:477027", "title": "Goodpasture's syndrome--effects of plasmapheresis.", "content": "Two patients with Goodpasture's syndrome, who underwent plasmapheresis are presented. One patient received prednisone but the other patient received no concomitant cytotoxic therapy. Results indicate that plasmapheresis reduces morbidity in patients with Goodpasture's syndrome. The possible mode of action of plasmapheresis is discussed. The use of cytotoxic  therapy as an adjunct to plasmapheresis may not be necessary since the anti-GBM production appears to be a self-limiting process.", "contents": "Goodpasture's syndrome--effects of plasmapheresis. Two patients with Goodpasture's syndrome, who underwent plasmapheresis are presented. One patient received prednisone but the other patient received no concomitant cytotoxic therapy. Results indicate that plasmapheresis reduces morbidity in patients with Goodpasture's syndrome. The possible mode of action of plasmapheresis is discussed. The use of cytotoxic  therapy as an adjunct to plasmapheresis may not be necessary since the anti-GBM production appears to be a self-limiting process."}
{"id": "PMID:477026", "title": "Intravenous immunoglobulin therapy for antibody deficiency.", "content": "Twenty patients with antibody deficiency were treated at random with either intramuscular immune serum globulin (ISG) or intravenous modified immune serum globulin (M-ISG). Fourteen patients received of 259 M-ISG infusions during 242 months of treatment. Catastrophic vasomotor reactions were not observed. A single dose of 150 mg/kilo M-ISG increased serum IgG values a mean 248 mg%. Intravenous M-ISG therapy was effective in reducing the incidence of acute infections. Subjects receiving M-ISG developed 0.103 acute infections per month of treatment. Patients injected with ISG had 0.295 acute infections per month of treatment. Seven subjects had separate courses of both intravenous M-ISG and intramuscular ISG. Acute infections per month of treatment for M-ISG and ISG were 0.104 and 0.406, respectively.", "contents": "Intravenous immunoglobulin therapy for antibody deficiency. Twenty patients with antibody deficiency were treated at random with either intramuscular immune serum globulin (ISG) or intravenous modified immune serum globulin (M-ISG). Fourteen patients received of 259 M-ISG infusions during 242 months of treatment. Catastrophic vasomotor reactions were not observed. A single dose of 150 mg/kilo M-ISG increased serum IgG values a mean 248 mg%. Intravenous M-ISG therapy was effective in reducing the incidence of acute infections. Subjects receiving M-ISG developed 0.103 acute infections per month of treatment. Patients injected with ISG had 0.295 acute infections per month of treatment. Seven subjects had separate courses of both intravenous M-ISG and intramuscular ISG. Acute infections per month of treatment for M-ISG and ISG were 0.104 and 0.406, respectively."}
{"id": "PMID:477025", "title": "Transfer factor and hepatitis B: a double blind study.", "content": "A prospective, double blind placebo-controlled trial was carried out on twenty-nine patients with hepatitis B. Thirteen received transfer factor and sixteen placebo. There were no significant differences between the two groups in any clinical or laboratory measurements made, although a rapid early reduction of serum aspartate transaminase levels by transfer factor is possible. Similarly, no significant changes were delineated by the in vitro measurements of lymphocyte function. Transfer factor did not alter the natural course of hepatitis B.", "contents": "Transfer factor and hepatitis B: a double blind study. A prospective, double blind placebo-controlled trial was carried out on twenty-nine patients with hepatitis B. Thirteen received transfer factor and sixteen placebo. There were no significant differences between the two groups in any clinical or laboratory measurements made, although a rapid early reduction of serum aspartate transaminase levels by transfer factor is possible. Similarly, no significant changes were delineated by the in vitro measurements of lymphocyte function. Transfer factor did not alter the natural course of hepatitis B."}
{"id": "PMID:477028", "title": "A comparison of two 125I C1q binding tests to detect soluble immune complexes in serum of patients with malignant disease.", "content": "The 125I C1q deviation test and the modified 125I C1q PEG precipitation test were compared in their ability to detect soluble immune complexes in serum using a model system of HSA-rabbit-anti-HSA, and were then applied to sera collected from patients with malignant and non-malignant conditions. Despite close agreement in the model system, the two tests gave divergent results for the presence of C1q binding substances in individual serum samples collected from patients. The inherent complexities of interpreting C1q binding in serum, in terms of the presence of soluble immune complexes, makes it questionable whether either test can be relied upon to provide a means of identifying these complexes in the sera from patients with malignant disease.", "contents": "A comparison of two 125I C1q binding tests to detect soluble immune complexes in serum of patients with malignant disease. The 125I C1q deviation test and the modified 125I C1q PEG precipitation test were compared in their ability to detect soluble immune complexes in serum using a model system of HSA-rabbit-anti-HSA, and were then applied to sera collected from patients with malignant and non-malignant conditions. Despite close agreement in the model system, the two tests gave divergent results for the presence of C1q binding substances in individual serum samples collected from patients. The inherent complexities of interpreting C1q binding in serum, in terms of the presence of soluble immune complexes, makes it questionable whether either test can be relied upon to provide a means of identifying these complexes in the sera from patients with malignant disease."}
{"id": "PMID:477029", "title": "Maternal smoking and cord blood immunity function.", "content": "Thymidine uptake in PHA-stimulated culture of cord blood cells from smoking mothers is greater than that from non-smoking mothers. There was no such difference when separated lymphocytes were studied; this suggests that smoking suppresses a suppressor cell. No difference was detected in haemoglobin, immunoglobulins, transferrin, cell counts, E-rosette counts and leucocyte mobility.", "contents": "Maternal smoking and cord blood immunity function. Thymidine uptake in PHA-stimulated culture of cord blood cells from smoking mothers is greater than that from non-smoking mothers. There was no such difference when separated lymphocytes were studied; this suggests that smoking suppresses a suppressor cell. No difference was detected in haemoglobin, immunoglobulins, transferrin, cell counts, E-rosette counts and leucocyte mobility."}
{"id": "PMID:477030", "title": "The effect of cortisone acetate on pulmonary lesions in a rabbit model of hypersensitivity pneumonitis (cortisone and hypersensitivity pneumonitis).", "content": "Rabbits were sensitized with Micropolyspora faeni by intratracheal inoculations and later challenged with the same antigen either with or without parenteral administration of cortisone acetate prior to challenge. Animals developed anti-M. faeni serum precipitins, M. faeni-induced alveolar macrophage migration inhibition, and positive 48-hr skin reactivity to M. faeni. Sensitized animals also demonstrated an augmented pulmonary histopathological response following respiratory challenge with M. faeni when compared to non-sensitized controls. Cortisone acetate abrogated this augmented pulmonary histopathological response following challenge with M. faeni. Cortisone acetate also abolished the positive alveolar macrophage migration inhibition found in sensitized animals.", "contents": "The effect of cortisone acetate on pulmonary lesions in a rabbit model of hypersensitivity pneumonitis (cortisone and hypersensitivity pneumonitis). Rabbits were sensitized with Micropolyspora faeni by intratracheal inoculations and later challenged with the same antigen either with or without parenteral administration of cortisone acetate prior to challenge. Animals developed anti-M. faeni serum precipitins, M. faeni-induced alveolar macrophage migration inhibition, and positive 48-hr skin reactivity to M. faeni. Sensitized animals also demonstrated an augmented pulmonary histopathological response following respiratory challenge with M. faeni when compared to non-sensitized controls. Cortisone acetate abrogated this augmented pulmonary histopathological response following challenge with M. faeni. Cortisone acetate also abolished the positive alveolar macrophage migration inhibition found in sensitized animals."}
{"id": "PMID:477032", "title": "Influence of supernatants from polymorphonuclear leucocytes on blastogenesis of syngeneic and allogeneic murine splenocytes.", "content": "Supernatant was produced from activated peritoneal polymorphonuclear leucocyte-rich cell populations from different strains of mice. These supernatants were studied for their ability to modify spontaneous and mitogen-induced blastogenesis of syngeneic and allogeneic splenocytes. Our results indicate that polymorphonuclear leucocyte-rich cell cultures from two strains of mice, A/J and BALB/c, produced a supernatant that could enhance PHA-induced blastogenesis of syngeneic and allogeneic splenocytes. Cells from a third strain C57B1/6, did not produce an active supernatant. In general, the response by splenocytes from these three strains paralleled the production of active supernatant that we observed. The response to the active supernatant was dependent upon the mitogen stimulation of the splenocytes, the mitogen dilution and the supernatant activity. These functions are believed to be genetically determined.", "contents": "Influence of supernatants from polymorphonuclear leucocytes on blastogenesis of syngeneic and allogeneic murine splenocytes. Supernatant was produced from activated peritoneal polymorphonuclear leucocyte-rich cell populations from different strains of mice. These supernatants were studied for their ability to modify spontaneous and mitogen-induced blastogenesis of syngeneic and allogeneic splenocytes. Our results indicate that polymorphonuclear leucocyte-rich cell cultures from two strains of mice, A/J and BALB/c, produced a supernatant that could enhance PHA-induced blastogenesis of syngeneic and allogeneic splenocytes. Cells from a third strain C57B1/6, did not produce an active supernatant. In general, the response by splenocytes from these three strains paralleled the production of active supernatant that we observed. The response to the active supernatant was dependent upon the mitogen stimulation of the splenocytes, the mitogen dilution and the supernatant activity. These functions are believed to be genetically determined."}
{"id": "PMID:477031", "title": "Electrophoretic behaviour of blood and synovial fluid lymphocytes in rheumatoid arthritis.", "content": "Probit analysis of the electrophoretic mobilities of human blood lymphocytes identifies at least three main subpopulations. According to their rate of movement in an electrical field, the subpopulations are referred to as the fast, intermediate and slow cell distributions. Lymphocytes of the fast and intermediate populations appear to be T cells, while the slow cell population includes cells with B cell characteristics. Compared with normal subjects, lymphocytes of intermediate mobility are significantly increased in the blood of rheumatoid patients and comprise a major fraction of the lymphocyte exudate in rheumatoid synovial fluid.", "contents": "Electrophoretic behaviour of blood and synovial fluid lymphocytes in rheumatoid arthritis. Probit analysis of the electrophoretic mobilities of human blood lymphocytes identifies at least three main subpopulations. According to their rate of movement in an electrical field, the subpopulations are referred to as the fast, intermediate and slow cell distributions. Lymphocytes of the fast and intermediate populations appear to be T cells, while the slow cell population includes cells with B cell characteristics. Compared with normal subjects, lymphocytes of intermediate mobility are significantly increased in the blood of rheumatoid patients and comprise a major fraction of the lymphocyte exudate in rheumatoid synovial fluid."}
{"id": "PMID:477034", "title": "Impaired neutrophil chemotaxis in Pelger-Hu\u00ebt anomaly.", "content": "A family has previously been described in which four members with Pelger-Hu\u00ebt (P-H) anomaly suffered from recurrent attacks of abdominal pain and fever, while one member, whose polymorphonuclear leucocytes (PMNs) were also hyposegmented, was asymptomatic. We studied chemotaxis, chemokinesis and spontaneous locomotion of PMNs in the three surviving symptomatic sisters, in their asymptomatic brother and in two asymptomatic members of another family with P-H anomaly. The spontaneous migration of the PMNs of the three sisters was significantly slower both under agarose and in a membrane filter than that of the PMNs of the asymptomatic patients with P-H anomaly. Chemotactic and chemokinetic locomotion of the PMNs of the symptomatic sisters was also slow. Our results suggests that the impaired chemotaxis was due to a defect in the intrinsic locomotor capacity of PMNs rather than in their deformability or their responsiveness to the chemotactic stimulus.", "contents": "Impaired neutrophil chemotaxis in Pelger-Hu\u00ebt anomaly. A family has previously been described in which four members with Pelger-Hu\u00ebt (P-H) anomaly suffered from recurrent attacks of abdominal pain and fever, while one member, whose polymorphonuclear leucocytes (PMNs) were also hyposegmented, was asymptomatic. We studied chemotaxis, chemokinesis and spontaneous locomotion of PMNs in the three surviving symptomatic sisters, in their asymptomatic brother and in two asymptomatic members of another family with P-H anomaly. The spontaneous migration of the PMNs of the three sisters was significantly slower both under agarose and in a membrane filter than that of the PMNs of the asymptomatic patients with P-H anomaly. Chemotactic and chemokinetic locomotion of the PMNs of the symptomatic sisters was also slow. Our results suggests that the impaired chemotaxis was due to a defect in the intrinsic locomotor capacity of PMNs rather than in their deformability or their responsiveness to the chemotactic stimulus."}
{"id": "PMID:477033", "title": "Elevated serum levels of a biliary glycoprotein (BGP I) in patients with liver or biliary tract disease.", "content": "Human hepatic bile contains a glycoprotein (biliary glycoprotein I, BGP I) which cross-reacts with the carcinoembryonic antigen (CEA). A radioimmunoassay for BGP I was developed. The interference of CEA or 'non-specific cross-reacting antigen' (NCA) in the assay was small. The serum levels of BGP I were determined in healthy subjects, in patients with hepato-biliary diseases and in patients with various infectious or inflammatory disorders. Healthy individuals, including pregnant women, had a serum BGP I concentration of about 0.5-1 mg/l. Diseases of the liver or biliary tract (e.g. hepatitis A or B, cytomegalovirus hepatitis, obstructive jaundice or primary biliary cirrhosis) were associated with elevated serum levels of BGP I, as opposed to infectious diseases not affecting the liver mostly showing values within the normal range. Raised levels of serum BGP I activity may reflect biliary obstruction as a result of interference with normal BGP I secretion to the bile.", "contents": "Elevated serum levels of a biliary glycoprotein (BGP I) in patients with liver or biliary tract disease. Human hepatic bile contains a glycoprotein (biliary glycoprotein I, BGP I) which cross-reacts with the carcinoembryonic antigen (CEA). A radioimmunoassay for BGP I was developed. The interference of CEA or 'non-specific cross-reacting antigen' (NCA) in the assay was small. The serum levels of BGP I were determined in healthy subjects, in patients with hepato-biliary diseases and in patients with various infectious or inflammatory disorders. Healthy individuals, including pregnant women, had a serum BGP I concentration of about 0.5-1 mg/l. Diseases of the liver or biliary tract (e.g. hepatitis A or B, cytomegalovirus hepatitis, obstructive jaundice or primary biliary cirrhosis) were associated with elevated serum levels of BGP I, as opposed to infectious diseases not affecting the liver mostly showing values within the normal range. Raised levels of serum BGP I activity may reflect biliary obstruction as a result of interference with normal BGP I secretion to the bile."}
{"id": "PMID:477035", "title": "The time of day of antigen encounter influences the magnitude of the immune response.", "content": "Studies in the rat and in man have shown that the time of day at which an antigen is encountered has an influence on the expression of any subsequent cell-mediated immunity, when the response is measured after a fixed interval. This suggests that immune processes are modulated by intrinsic biological rhythms. Experiments are now reported in which sensitized rats were ear-challenged with oxazolone and studied at intervals during the delayed hypersensitivity reaction. These show that differences in the expression of immunity due to the clock time of challenge became apparent at an early stage and were still present after the maximum response, assessed by the change in ear thickness. The experiments also show that the circadian variations in antigen responsiveness are present during the second recall of immunity and can be manipulated by altering the lighting regimen. The light-dark cycle is often a synchronizer for biological rhythms, although its precise role in the oxazolone system remains to be evaluated. This study demonstrates that phase reversal of the lighting regimen alters the proportions of lymphocytes present in rat blood at two clock times, one of which is the time of day at which the maximum immune response to oxazolone is initiated.", "contents": "The time of day of antigen encounter influences the magnitude of the immune response. Studies in the rat and in man have shown that the time of day at which an antigen is encountered has an influence on the expression of any subsequent cell-mediated immunity, when the response is measured after a fixed interval. This suggests that immune processes are modulated by intrinsic biological rhythms. Experiments are now reported in which sensitized rats were ear-challenged with oxazolone and studied at intervals during the delayed hypersensitivity reaction. These show that differences in the expression of immunity due to the clock time of challenge became apparent at an early stage and were still present after the maximum response, assessed by the change in ear thickness. The experiments also show that the circadian variations in antigen responsiveness are present during the second recall of immunity and can be manipulated by altering the lighting regimen. The light-dark cycle is often a synchronizer for biological rhythms, although its precise role in the oxazolone system remains to be evaluated. This study demonstrates that phase reversal of the lighting regimen alters the proportions of lymphocytes present in rat blood at two clock times, one of which is the time of day at which the maximum immune response to oxazolone is initiated."}
{"id": "PMID:477044", "title": "Pseudohypoaldosteronism.", "content": "Three children, two of whom were siblings, had pseudohypoaldosteronism. The features of this condition include failure to thrive, hyperkalemia, metabolic acidosis, salt wasting, elevated peripheral renin activity, and increased plasma aldosterone concentration. Hyperplasia of juxtaglomerular apparatus was seen in a renal biopsy specimen from one of these patients. Administration of large quantities of salt normalized the serum electrolyte abnormalities and permitted normal growth. Furthermore, the serum electrolyte abnormalities were prevented by administration of increased amounts of salt to one of these children from birth.", "contents": "Pseudohypoaldosteronism. Three children, two of whom were siblings, had pseudohypoaldosteronism. The features of this condition include failure to thrive, hyperkalemia, metabolic acidosis, salt wasting, elevated peripheral renin activity, and increased plasma aldosterone concentration. Hyperplasia of juxtaglomerular apparatus was seen in a renal biopsy specimen from one of these patients. Administration of large quantities of salt normalized the serum electrolyte abnormalities and permitted normal growth. Furthermore, the serum electrolyte abnormalities were prevented by administration of increased amounts of salt to one of these children from birth."}
{"id": "PMID:477045", "title": "Bicarbonate hemodialysis using a sorbent regenerative system.", "content": "In light of the concern about the use of acetate in dialyzate a simplified means of performing acetate-free hemodialysis has been developed using the sorbent regenerative system (RED). Fifteen sorbent bicarbonate hemodialyses free of acetate were performed on two patients for 4 or 6 hours. The initial dialyzate mixture contained 135 mEq/l NaHCO3 only. The infusate cations of K, Ca and Mg were in the form of chloride salts. A 30-minute titration of the sorbent cartridge with the bicarbonate dialyzate was necessary to release the excessive hydrogen ion from the cartridge which allowed the dialyzate bicarbonate to stabilize. The dialyzate Na fell during titration but rose during dialysis. The dialyzate Cl rose throughout titration and dialyses. The plasma bicarbonate rose significantly by the end of 4 and 6 hour dialyses. The blood pO2 and pCO2 did not change during dialysis and a significant rise in blood pH resulted. We conclude that bicarbonate dialysis is feasible and relatively easily accomplished in the sorbent system.", "contents": "Bicarbonate hemodialysis using a sorbent regenerative system. In light of the concern about the use of acetate in dialyzate a simplified means of performing acetate-free hemodialysis has been developed using the sorbent regenerative system (RED). Fifteen sorbent bicarbonate hemodialyses free of acetate were performed on two patients for 4 or 6 hours. The initial dialyzate mixture contained 135 mEq/l NaHCO3 only. The infusate cations of K, Ca and Mg were in the form of chloride salts. A 30-minute titration of the sorbent cartridge with the bicarbonate dialyzate was necessary to release the excessive hydrogen ion from the cartridge which allowed the dialyzate bicarbonate to stabilize. The dialyzate Na fell during titration but rose during dialysis. The dialyzate Cl rose throughout titration and dialyses. The plasma bicarbonate rose significantly by the end of 4 and 6 hour dialyses. The blood pO2 and pCO2 did not change during dialysis and a significant rise in blood pH resulted. We conclude that bicarbonate dialysis is feasible and relatively easily accomplished in the sorbent system."}
{"id": "PMID:477046", "title": "Serum C-peptide in renal failure patients following stimulation of pancreatic secretion.", "content": "We have demonstrated persistently elevated serum C-peptide concentrations in patients with chronic renal failure on chronic hemodialysis. A blunted serum C-peptide response to intravenous glucose, glucagon and tolbutamide was also found. However, the response to oral glucose stimulation was greater and more prolonged than in control subjects, probably related to the magnitude of hyperglycemia found in patients with chronic renal failure. These observations suggest the existence of a defect in the renal clearance of C-peptide although an abnormality in C-peptide secretion cannot be excluded.", "contents": "Serum C-peptide in renal failure patients following stimulation of pancreatic secretion. We have demonstrated persistently elevated serum C-peptide concentrations in patients with chronic renal failure on chronic hemodialysis. A blunted serum C-peptide response to intravenous glucose, glucagon and tolbutamide was also found. However, the response to oral glucose stimulation was greater and more prolonged than in control subjects, probably related to the magnitude of hyperglycemia found in patients with chronic renal failure. These observations suggest the existence of a defect in the renal clearance of C-peptide although an abnormality in C-peptide secretion cannot be excluded."}
{"id": "PMID:477049", "title": "The Ask-Upmark kidney in a premature infant.", "content": "A premature infant with segmental hypoplasia of one kidney is reported. Since 1929 when Ask-Upmark first reported this renal anomaly and its association with hypertension, the nature of the lesion has remained in doubt. The question whether it is acquired post-natally or whether it is a developmental mal-formation has not been resolved. The case which we now report clearly documents the prenatal development of the lesion as well as its association with arrested nephrogenesis and the presence of abnormal vasculature. The cause of these changes is not clear, but it is suggested that ureteral reflux was present.", "contents": "The Ask-Upmark kidney in a premature infant. A premature infant with segmental hypoplasia of one kidney is reported. Since 1929 when Ask-Upmark first reported this renal anomaly and its association with hypertension, the nature of the lesion has remained in doubt. The question whether it is acquired post-natally or whether it is a developmental mal-formation has not been resolved. The case which we now report clearly documents the prenatal development of the lesion as well as its association with arrested nephrogenesis and the presence of abnormal vasculature. The cause of these changes is not clear, but it is suggested that ureteral reflux was present."}
{"id": "PMID:477048", "title": "Inconsistency in radiographic evaluation of progressive renal osteodystrophy.", "content": "Five radiologists graded 49 series of bone X-rays of 20 patients with chronic renal failure treated by hemodialysis. There was a high incidence of osteodystrophy which progressed identifiably over intervals exceeding 12 months. The severity grade of osteodystrophy was poorly reproducible among patients, among radiologists, and even between interpretations by the same radiologist after an interval of 10 months. Although the severity of osteodystrophy correlated with serum alkaline phosphatase values, the latter was not an accurate predictor of the severity of the bone lesions. Radiographic reassessment at intervals of one year or less in the asymptomatic patient has less reproducibility than the anticipated changes. More sensitive and reliable techniques are recommended.", "contents": "Inconsistency in radiographic evaluation of progressive renal osteodystrophy. Five radiologists graded 49 series of bone X-rays of 20 patients with chronic renal failure treated by hemodialysis. There was a high incidence of osteodystrophy which progressed identifiably over intervals exceeding 12 months. The severity grade of osteodystrophy was poorly reproducible among patients, among radiologists, and even between interpretations by the same radiologist after an interval of 10 months. Although the severity of osteodystrophy correlated with serum alkaline phosphatase values, the latter was not an accurate predictor of the severity of the bone lesions. Radiographic reassessment at intervals of one year or less in the asymptomatic patient has less reproducibility than the anticipated changes. More sensitive and reliable techniques are recommended."}
{"id": "PMID:477050", "title": "Inadequate removal of methanol and formate using the sorbent based regeneration hemodialysis delivery system.", "content": "The sorbent based hemodialysis delivery system was shown to be ineffective for the treatment of methanol intoxication. Clinical and in vitro studies showed rapid saturation of the sorbent cartridge for methanol and formate adsorption. Methanol and formate clearances were four fold less than achieved with the recirculating single pass delivery system. The sorbent delivery system should not be used for methanol intoxication.", "contents": "Inadequate removal of methanol and formate using the sorbent based regeneration hemodialysis delivery system. The sorbent based hemodialysis delivery system was shown to be ineffective for the treatment of methanol intoxication. Clinical and in vitro studies showed rapid saturation of the sorbent cartridge for methanol and formate adsorption. Methanol and formate clearances were four fold less than achieved with the recirculating single pass delivery system. The sorbent delivery system should not be used for methanol intoxication."}
{"id": "PMID:477051", "title": "The influence of several factors that intervene in hemodialysis on serum levels of triglycerides and free fatty acids.", "content": "The effect of the administration of heparin and glucose, in doses and sequence similar to those of hemodialysis, on serum free fatty acids and triglycerides, was studied in eight patients with chronic renal failure who had not undergone hemodialysis. The results are compared with the action exerted by hemodialysis on the same parameters. During hemodialysis, the values of triglycerides are not significantly different from those obtained at similar intervals after the administration of glucose and/or heparin, with the exception that the values obtained five hours after beginning hemodialysis are lower than those obtained five hours after the administration of glucose (P less than 0.005). The levels of free fatty acids obtained during hemodialysis are higher (P less than 0.005) than those found during the other tests. The possible origin of these high fatty acid values is discussed, and the possibility that they originate from the acetate in the dialysis liquid is suggested.", "contents": "The influence of several factors that intervene in hemodialysis on serum levels of triglycerides and free fatty acids. The effect of the administration of heparin and glucose, in doses and sequence similar to those of hemodialysis, on serum free fatty acids and triglycerides, was studied in eight patients with chronic renal failure who had not undergone hemodialysis. The results are compared with the action exerted by hemodialysis on the same parameters. During hemodialysis, the values of triglycerides are not significantly different from those obtained at similar intervals after the administration of glucose and/or heparin, with the exception that the values obtained five hours after beginning hemodialysis are lower than those obtained five hours after the administration of glucose (P less than 0.005). The levels of free fatty acids obtained during hemodialysis are higher (P less than 0.005) than those found during the other tests. The possible origin of these high fatty acid values is discussed, and the possibility that they originate from the acetate in the dialysis liquid is suggested."}
{"id": "PMID:477052", "title": "Aluminum, zinc and copper concentrations in plasma in chronic renal insufficiency.", "content": "Plasma aluminium, zinc and copper were determined in 68 patients with chronic renal insufficiency and in 20 healthy individuals. In the renal insufficiency group 16 patients had received aluminium containing substances particularly phosphate binding drugs (aluminium hydroxide) for a long time. We observed that only the patients taking aluminium hydroxide showed elevated aluminium levels in plasma (controls: 0.86 +/- 0.27 mumoles/l; renal insufficiency: 3.05 +/- 1.63 mumoles/l). In two of these patients with symptoms of \"dialysis dementia\", plasma aluminium concentrations were markedly increased. The zinc concentrations in plasma of patients with renal insufficiency were slightly decreased (controls: 14.48 +/- 2.65 mumoles/l; renal insufficiency: 11.77 +/- 2.35 mumoles/l); the plasma copper concentrations were slightly increased (controls: 17.3 +/- 2.83 mumoles/l; renal insufficiency: 22.98 +/- 4.7 mumoles/l). There was a distinct decrease of plasma zinc concentrations in patients with raised aluminium levels. The clinical aspects of these changes are discussed.", "contents": "Aluminum, zinc and copper concentrations in plasma in chronic renal insufficiency. Plasma aluminium, zinc and copper were determined in 68 patients with chronic renal insufficiency and in 20 healthy individuals. In the renal insufficiency group 16 patients had received aluminium containing substances particularly phosphate binding drugs (aluminium hydroxide) for a long time. We observed that only the patients taking aluminium hydroxide showed elevated aluminium levels in plasma (controls: 0.86 +/- 0.27 mumoles/l; renal insufficiency: 3.05 +/- 1.63 mumoles/l). In two of these patients with symptoms of \"dialysis dementia\", plasma aluminium concentrations were markedly increased. The zinc concentrations in plasma of patients with renal insufficiency were slightly decreased (controls: 14.48 +/- 2.65 mumoles/l; renal insufficiency: 11.77 +/- 2.35 mumoles/l); the plasma copper concentrations were slightly increased (controls: 17.3 +/- 2.83 mumoles/l; renal insufficiency: 22.98 +/- 4.7 mumoles/l). There was a distinct decrease of plasma zinc concentrations in patients with raised aluminium levels. The clinical aspects of these changes are discussed."}
{"id": "PMID:477053", "title": "Incidence and significance of rising blood acetate levels during hemodialysis.", "content": "Recent work suggests that rising arterial acetate levels occur in some patients undergoing hemodialysis and that they may be responsible for some dialysis problems, particularly cardiovascular instability. Blood acetate levels and acetate flux rates have been determined in 20 adult and 4 pediatric patients during hemodialysis as well as in 4 patients with combined renal and hepatic failure. Rising acetate levels occurred in 25% of the adult patients, although they were stable in the children and the patients with renal and hepatic failure. The occurrence of hypotension during dialysis was unrelated to a high blood acetate level.", "contents": "Incidence and significance of rising blood acetate levels during hemodialysis. Recent work suggests that rising arterial acetate levels occur in some patients undergoing hemodialysis and that they may be responsible for some dialysis problems, particularly cardiovascular instability. Blood acetate levels and acetate flux rates have been determined in 20 adult and 4 pediatric patients during hemodialysis as well as in 4 patients with combined renal and hepatic failure. Rising acetate levels occurred in 25% of the adult patients, although they were stable in the children and the patients with renal and hepatic failure. The occurrence of hypotension during dialysis was unrelated to a high blood acetate level."}
{"id": "PMID:477054", "title": "Acute massive hydrothorax complicating peritoneal dialysis, report of 2 cases and a review of the literature.", "content": "Acute massive right-sided hydrothorax is a relatively rare but serious complication of peritoneal dialysis and may be responsible for the development of dyspnea during peritoneal dialysis. The pleural fluid appears to arise from the peritoneal dialyzate based on the time of its appearance and its chemical composition. It should be included in the differential diagnosis when a patient becomes dyspneic during peritoneal dialysis along with exaccerbation of congestive heart failure, pneumonia, atelectasis, and purulent bronchitis. Its occurrence is an indication to stop the peritoneal dialysis and contraindicates further use of this form of dialysis. Treatment may be conservative or aggressive (thoracentesis) depending on the clinical condition of the patient. Etiology is poorly understood. In some cases, there may be traumatic diaphragmatic fenestrations, but the majority of cases appear to be due to less well defined communications between the peritoneal and pleural spaces.", "contents": "Acute massive hydrothorax complicating peritoneal dialysis, report of 2 cases and a review of the literature. Acute massive right-sided hydrothorax is a relatively rare but serious complication of peritoneal dialysis and may be responsible for the development of dyspnea during peritoneal dialysis. The pleural fluid appears to arise from the peritoneal dialyzate based on the time of its appearance and its chemical composition. It should be included in the differential diagnosis when a patient becomes dyspneic during peritoneal dialysis along with exaccerbation of congestive heart failure, pneumonia, atelectasis, and purulent bronchitis. Its occurrence is an indication to stop the peritoneal dialysis and contraindicates further use of this form of dialysis. Treatment may be conservative or aggressive (thoracentesis) depending on the clinical condition of the patient. Etiology is poorly understood. In some cases, there may be traumatic diaphragmatic fenestrations, but the majority of cases appear to be due to less well defined communications between the peritoneal and pleural spaces."}
{"id": "PMID:477056", "title": "The antibody response in chronic pyelonephritis.", "content": "Serum antibodies specific for the bacteria isolated from the urines of 41 patients with chronic pyelonephritis and 14 with asymptomatic bacteriuria were assayed. Even though it is clear that infections which involve the kidney evoke higher antibody responses than do purely bladder infections, it is difficult to establish a cut-off level for distinguishing the two by this method. There is a greater probability that there will be an upper urinary tract infection without an antibody response than that there will be a good antibody response in cases with lower tract infections. In our case list, 49% of the patients with chronic pyelonephritis had titers equal to or greater than 1:400, and another 44% had titers between 1:50 and 1:100. Therefore it appears that the magnitude of the antibody response depends not only on the location of the infection but also on other factors, such as the intrinsic immunogenicity of the different bacterial strains and the properties of the immunological system of the patient.", "contents": "The antibody response in chronic pyelonephritis. Serum antibodies specific for the bacteria isolated from the urines of 41 patients with chronic pyelonephritis and 14 with asymptomatic bacteriuria were assayed. Even though it is clear that infections which involve the kidney evoke higher antibody responses than do purely bladder infections, it is difficult to establish a cut-off level for distinguishing the two by this method. There is a greater probability that there will be an upper urinary tract infection without an antibody response than that there will be a good antibody response in cases with lower tract infections. In our case list, 49% of the patients with chronic pyelonephritis had titers equal to or greater than 1:400, and another 44% had titers between 1:50 and 1:100. Therefore it appears that the magnitude of the antibody response depends not only on the location of the infection but also on other factors, such as the intrinsic immunogenicity of the different bacterial strains and the properties of the immunological system of the patient."}
{"id": "PMID:477060", "title": "Compression osteotomy of the tibia.", "content": "Compression osteotomy of the tibia is to allow knee motion postoperatively without case immobilization. Rigid internal stabilization by axial compression and tension band wiring enhances union. The rigid internal stabilization prevents loss of position of the osteotomy, often seen in other techniques. The benchmark for this osteotomy operation is no fractures of the osteotomy fragments, no position loss, no thromboemboli, and no deep wound infections.", "contents": "Compression osteotomy of the tibia. Compression osteotomy of the tibia is to allow knee motion postoperatively without case immobilization. Rigid internal stabilization by axial compression and tension band wiring enhances union. The rigid internal stabilization prevents loss of position of the osteotomy, often seen in other techniques. The benchmark for this osteotomy operation is no fractures of the osteotomy fragments, no position loss, no thromboemboli, and no deep wound infections."}
{"id": "PMID:477061", "title": "The use of the Vidal-Adrey external fixation system. Part 1: The treatment of open fractures.", "content": "The average time for healing of open fractures treated by the Vidal-Adrey external fixation method was 9 1/2 months for the lower extremity and 4 months for the upper extremity injuries. In a series of 33 cases, the results were excellent or good in 21.", "contents": "The use of the Vidal-Adrey external fixation system. Part 1: The treatment of open fractures. The average time for healing of open fractures treated by the Vidal-Adrey external fixation method was 9 1/2 months for the lower extremity and 4 months for the upper extremity injuries. In a series of 33 cases, the results were excellent or good in 21."}
{"id": "PMID:477062", "title": "Computed tomography in the diagnosis of dislocations of the sternoclavicular joint.", "content": "Dislocations of the sternoclavicular joint can be easily characterized with computed tomography. Two typical cases, one demonstrating superior dislocation and the other showing posterior dislocation of the clavicle, illustrate the value of the method. Correlated anatomical observations demonstrate the proximity of the great vessels to the sternoclavicular joint.", "contents": "Computed tomography in the diagnosis of dislocations of the sternoclavicular joint. Dislocations of the sternoclavicular joint can be easily characterized with computed tomography. Two typical cases, one demonstrating superior dislocation and the other showing posterior dislocation of the clavicle, illustrate the value of the method. Correlated anatomical observations demonstrate the proximity of the great vessels to the sternoclavicular joint."}
{"id": "PMID:477063", "title": "The use of the Vidal-Adrey external fixation system. Part 2: The treatment of infected and previously infected pseudarthrosis.", "content": "A total of 23 infected or previously infected non-unions were treated by the Vidal-Adrey modification of the Hoffmann apparatus. The external fixators were applied after decortication and bone grafting either immediately in cases with no evidence of infection or later in cases of draining, infected wounds. The average healing time for these non-unions, that is time when no external support was required, was 12 months after the initial application of the Vidal-Adrey external fixators. The results were considered good or acceptable in 21 of 23 cases. Union occurred in 21, although union did not necessarily mean a good result overall when all other factors (shortening, etc.) were considered. This fixation device, when correctly applied and in conjuction with adequate debridement and bone grafting is an excellent method for the treatment of severe non-unions where infection is or was a problem.", "contents": "The use of the Vidal-Adrey external fixation system. Part 2: The treatment of infected and previously infected pseudarthrosis. A total of 23 infected or previously infected non-unions were treated by the Vidal-Adrey modification of the Hoffmann apparatus. The external fixators were applied after decortication and bone grafting either immediately in cases with no evidence of infection or later in cases of draining, infected wounds. The average healing time for these non-unions, that is time when no external support was required, was 12 months after the initial application of the Vidal-Adrey external fixators. The results were considered good or acceptable in 21 of 23 cases. Union occurred in 21, although union did not necessarily mean a good result overall when all other factors (shortening, etc.) were considered. This fixation device, when correctly applied and in conjuction with adequate debridement and bone grafting is an excellent method for the treatment of severe non-unions where infection is or was a problem."}
{"id": "PMID:477064", "title": "Familial osteochondritis dissecans of the knee.", "content": "The autosomal dominant pattern of osteochondritis dissecans and association with mild dwarfism were observed during a study of 4 generations of one family. Twelve of 31 family members had proven osteochondritis dissecans and 8 others appeared to have or (now deceased) have had it; 10 of these 20 patients were of short stature (less than 5%). At the onset, children under 11 years of age had bilateral irregularities of ossification. Three of the 11 adolescents (13-19 years) had osteochondritis dissecans with development of the condition from irregularities of ossification that could be traced in the roentgenograph of one. Two of the 3 surviving adults now in their sixth or seventh decade have osteoarthritis (one moderate, one severe). The younger adults, now in the 20's and 40's, have minimal knee sysmptoms.", "contents": "Familial osteochondritis dissecans of the knee. The autosomal dominant pattern of osteochondritis dissecans and association with mild dwarfism were observed during a study of 4 generations of one family. Twelve of 31 family members had proven osteochondritis dissecans and 8 others appeared to have or (now deceased) have had it; 10 of these 20 patients were of short stature (less than 5%). At the onset, children under 11 years of age had bilateral irregularities of ossification. Three of the 11 adolescents (13-19 years) had osteochondritis dissecans with development of the condition from irregularities of ossification that could be traced in the roentgenograph of one. Two of the 3 surviving adults now in their sixth or seventh decade have osteoarthritis (one moderate, one severe). The younger adults, now in the 20's and 40's, have minimal knee sysmptoms."}
{"id": "PMID:477065", "title": "Late results of Hauser procedure for recurrent dislocation of the patella.", "content": "To determine whether the Hauser procedure for recurrent dislocation of the patella prevents repeated dislocation and provides a functional knee without late degenerative arthritis, 52 patients with 67 surgically treated knees were studied clinically and roentgenographically with an average follow-up of 17.1 years. Greater than 70% of patients had an excellent or good result up to as long as 30 years postoperatively. There appeared to be no predisposition to the development of degenerative arthritis. Recurrent dislocation persisted in 7% of cases and female patients with generalized ligamentous laxity had a high incidence of persistant, recurrent dislocation and poorer long-term results. Development of genu recurvatum is a definite risk in patients who have tibial tubercle transfer prior to epiphyseal plate closure.", "contents": "Late results of Hauser procedure for recurrent dislocation of the patella. To determine whether the Hauser procedure for recurrent dislocation of the patella prevents repeated dislocation and provides a functional knee without late degenerative arthritis, 52 patients with 67 surgically treated knees were studied clinically and roentgenographically with an average follow-up of 17.1 years. Greater than 70% of patients had an excellent or good result up to as long as 30 years postoperatively. There appeared to be no predisposition to the development of degenerative arthritis. Recurrent dislocation persisted in 7% of cases and female patients with generalized ligamentous laxity had a high incidence of persistant, recurrent dislocation and poorer long-term results. Development of genu recurvatum is a definite risk in patients who have tibial tubercle transfer prior to epiphyseal plate closure."}
{"id": "PMID:477067", "title": "Bilateral total knee replacement under one anesthetic.", "content": "The postoperative results and complications among 41 patients undergoing bilateral total knee arthroplasty were compared with 40 patients who underwent unilateral total knee replacement. Among the bilateral group, the most common diagnosis was rheumatoid arthritis (68%), while degenerative arthritis prevailed in the unilateral group (50%). Preoperatively, patients in the bilateral group exhibited generally a greater degree of physical disability and limitation of activity due to multiple joint involvement. Postoperatively, no change in the arc of motion was noted in either group, although flexion contractures were significantly diminished in both. The degree of postoperative pain relief was identical in both groups as was the decreased need for walking devices. The most commonly observed complication among the unilateral group was prosthetic loosening (12.%). This was felt explainable on the basis of the increased postoperative activity level of patients with degenerative arthritis and single joint disease. Patients in the bilateral group showed a higher incidence of wound problems, presumably secondary to the long-term use of steroids. The infection rate was identical in each group, but the bilateral group displayed a higher incidence of pulmonary emboli (9.7 vs. 2.5%). The postoperative need for rehabilitation services and the duration of time before dependent walking was achieved was not prolonged in the bilateral group. Hospitalization of the bilateral group was increased only 20%. No deaths were reported in either group. When the need for total knee replacement exists bilaterally, bilateral replacement with a single anesthetic would appear reasonable, provided careful patient selection and technical guidelines are followed.", "contents": "Bilateral total knee replacement under one anesthetic. The postoperative results and complications among 41 patients undergoing bilateral total knee arthroplasty were compared with 40 patients who underwent unilateral total knee replacement. Among the bilateral group, the most common diagnosis was rheumatoid arthritis (68%), while degenerative arthritis prevailed in the unilateral group (50%). Preoperatively, patients in the bilateral group exhibited generally a greater degree of physical disability and limitation of activity due to multiple joint involvement. Postoperatively, no change in the arc of motion was noted in either group, although flexion contractures were significantly diminished in both. The degree of postoperative pain relief was identical in both groups as was the decreased need for walking devices. The most commonly observed complication among the unilateral group was prosthetic loosening (12.%). This was felt explainable on the basis of the increased postoperative activity level of patients with degenerative arthritis and single joint disease. Patients in the bilateral group showed a higher incidence of wound problems, presumably secondary to the long-term use of steroids. The infection rate was identical in each group, but the bilateral group displayed a higher incidence of pulmonary emboli (9.7 vs. 2.5%). The postoperative need for rehabilitation services and the duration of time before dependent walking was achieved was not prolonged in the bilateral group. Hospitalization of the bilateral group was increased only 20%. No deaths were reported in either group. When the need for total knee replacement exists bilaterally, bilateral replacement with a single anesthetic would appear reasonable, provided careful patient selection and technical guidelines are followed."}
{"id": "PMID:477066", "title": "GUEPAR knee arthroplasty results and late complications.", "content": "One hundred eight GUEPAR knee arthroplasties have been studied with a follow-up of one year on 41; 2 years on 22 and 3 years on 45 knees. Overall results were 17% excellent, 44% good, 10% fair and 29% poor. Excellent results were comparatively fewer in rheumatoid arthritis. There was a deterioration in the quality of results of arthroplasty with longer follow-up. The incidence of deep infection was 11%. There was a significant correlation between early wound drainage and deep infection. More than half of the infected knees have not required intervenition as yet. One knee was revised and 3 had attempted arthrodesis with one successful fusion. Two patients died with septicemia. Axle migration occurred in 8 knees and femoral stem breakage in 2 knees. Loosening was found in 27% of the knees with progressive reduction in quality of the arthroplasty. Incomplete cementing predisposed to loosening. Patellar symptoms were present in 28% of the knees. Patellar subluxation and dislocation occurred in 49% of the knees. More than half of these were symptomatic. With normal patellofemoral alignment, pain was more common in the osteoarthritic knee. Use of a patellar implant with GUEPAR knee prostesis should be restricted to severely disabled patients with major fixed deformities. Mechanical failure can be minimized by proper positioning of the implant, correct alignment of the extensor mechanism and adequate cement around the entire stem.", "contents": "GUEPAR knee arthroplasty results and late complications. One hundred eight GUEPAR knee arthroplasties have been studied with a follow-up of one year on 41; 2 years on 22 and 3 years on 45 knees. Overall results were 17% excellent, 44% good, 10% fair and 29% poor. Excellent results were comparatively fewer in rheumatoid arthritis. There was a deterioration in the quality of results of arthroplasty with longer follow-up. The incidence of deep infection was 11%. There was a significant correlation between early wound drainage and deep infection. More than half of the infected knees have not required intervenition as yet. One knee was revised and 3 had attempted arthrodesis with one successful fusion. Two patients died with septicemia. Axle migration occurred in 8 knees and femoral stem breakage in 2 knees. Loosening was found in 27% of the knees with progressive reduction in quality of the arthroplasty. Incomplete cementing predisposed to loosening. Patellar symptoms were present in 28% of the knees. Patellar subluxation and dislocation occurred in 49% of the knees. More than half of these were symptomatic. With normal patellofemoral alignment, pain was more common in the osteoarthritic knee. Use of a patellar implant with GUEPAR knee prostesis should be restricted to severely disabled patients with major fixed deformities. Mechanical failure can be minimized by proper positioning of the implant, correct alignment of the extensor mechanism and adequate cement around the entire stem."}
{"id": "PMID:477069", "title": "Mycobacterium abscessus (M. chelonei) infection of the knee joint: report of two cases following intra-articular injection of corticosteroids.", "content": "In 2 patients (3 knees) acute infectious arthritis of the knee developed following intra-articular injection of corticosteroids. The pathogenic organism responsible for the infection was found to be Mycobacterium Abscessus (M. Chelonei). Diagnostic difficulties were encountered due to the rarity of such infections and elusive identification of the organism with routine laboratory procedures. Therapeutic approaches necessitated combined radical surgical procedures together with antituberculosis drugs. Surgical treatment also afforded correct and definitive identification of the organism, from material obtained at operation. Although these mycobacteriae were found to be resistent to antituberculosis drugs, these same drugs proved effective clinically.", "contents": "Mycobacterium abscessus (M. chelonei) infection of the knee joint: report of two cases following intra-articular injection of corticosteroids. In 2 patients (3 knees) acute infectious arthritis of the knee developed following intra-articular injection of corticosteroids. The pathogenic organism responsible for the infection was found to be Mycobacterium Abscessus (M. Chelonei). Diagnostic difficulties were encountered due to the rarity of such infections and elusive identification of the organism with routine laboratory procedures. Therapeutic approaches necessitated combined radical surgical procedures together with antituberculosis drugs. Surgical treatment also afforded correct and definitive identification of the organism, from material obtained at operation. Although these mycobacteriae were found to be resistent to antituberculosis drugs, these same drugs proved effective clinically."}
{"id": "PMID:477070", "title": "Chronic dislocation of the sternoclavicular joint: an operative repair.", "content": "Chronic spontaneous dislocation of the sternoclavicular joint occurs more frequently in athletes than nonathletes, causing weakness and sometimes pain during prolonged stress upon the arm. Five such joints were successfully treated in 4 patients by means of a dynamic method of repair--tenodesis of the sternal head of the sternomastoid muscle. The sternal origin of the sternomastoid muscle in continuity with its muscle belly and strip of sternal periosteum is looped under the first rib, back through a drill hole in the clavicle and sutured to itself to replace the damaged costoclavicular ligament. Dislocations of the joint should not be repaired for purely cosmetic reasons as the risk of keloid scar formation in this region is notoriously high. All the patients who were young and athletically inclined, recovered fully within 4 months of the operation and returned to their sporting activities. Follow-up has ranged from 12 to 38 months. There have been no late complications or failures of the technique.", "contents": "Chronic dislocation of the sternoclavicular joint: an operative repair. Chronic spontaneous dislocation of the sternoclavicular joint occurs more frequently in athletes than nonathletes, causing weakness and sometimes pain during prolonged stress upon the arm. Five such joints were successfully treated in 4 patients by means of a dynamic method of repair--tenodesis of the sternal head of the sternomastoid muscle. The sternal origin of the sternomastoid muscle in continuity with its muscle belly and strip of sternal periosteum is looped under the first rib, back through a drill hole in the clavicle and sutured to itself to replace the damaged costoclavicular ligament. Dislocations of the joint should not be repaired for purely cosmetic reasons as the risk of keloid scar formation in this region is notoriously high. All the patients who were young and athletically inclined, recovered fully within 4 months of the operation and returned to their sporting activities. Follow-up has ranged from 12 to 38 months. There have been no late complications or failures of the technique."}
{"id": "PMID:477071", "title": "Complete replacement of the peroneus longus muscle by a ganglion with compression of the peroneal nerve: a case report.", "content": "In a 38-year-old woman, the entire muscle belly of the peroneus longus was replaced by a ganglion. Signs of peroneal nerve dysfunction resulted from direct pressure of the ganglion. Excision of the ganglion required a tenodesis of the peroneus longus tendon to provide a normal gait pattern which was maintained during a 17 month follow-up period. This entity needs careful, prompt evaluation to avoid neurological damage and to distinguish it from compartment syndrome.", "contents": "Complete replacement of the peroneus longus muscle by a ganglion with compression of the peroneal nerve: a case report. In a 38-year-old woman, the entire muscle belly of the peroneus longus was replaced by a ganglion. Signs of peroneal nerve dysfunction resulted from direct pressure of the ganglion. Excision of the ganglion required a tenodesis of the peroneus longus tendon to provide a normal gait pattern which was maintained during a 17 month follow-up period. This entity needs careful, prompt evaluation to avoid neurological damage and to distinguish it from compartment syndrome."}
{"id": "PMID:477072", "title": "Surgical treatment of reruptures of the tendoachilles following nonsurgical treatment.", "content": "Nine patients who sustained a rerupture of their Achilles tendon after nonsurgical treatment were evaluated both subjectively and objectively with the Cybex isokinetic dynamometer. Subjectively, only 5 of the 8 patients were satisfied as compared to a 93% satisfaction rate in our previous series of patients with primary surgical repair. Cybex measurements were statistically inconsistent, unlike the primary repairs. This small series of patients in which the tendoachilles was reconstructed following failed nonsurgical treatment suggests that early surgical repair is the treatment of choice unless otherwise medically contraindicated.", "contents": "Surgical treatment of reruptures of the tendoachilles following nonsurgical treatment. Nine patients who sustained a rerupture of their Achilles tendon after nonsurgical treatment were evaluated both subjectively and objectively with the Cybex isokinetic dynamometer. Subjectively, only 5 of the 8 patients were satisfied as compared to a 93% satisfaction rate in our previous series of patients with primary surgical repair. Cybex measurements were statistically inconsistent, unlike the primary repairs. This small series of patients in which the tendoachilles was reconstructed following failed nonsurgical treatment suggests that early surgical repair is the treatment of choice unless otherwise medically contraindicated."}
{"id": "PMID:477073", "title": "The role of soft tissue reconstruction in flexible implant arthroplasty of the metacarpophalangeal joint.", "content": "In 116 hand metacarpophalangeal joint arthroplasties of the flexible type, the operative technique did not include reconstruction of capsular or ligamentous structures, but employed meticulous relocation of the common extensor tendons, and a modification of the metacarpal osteotomy plane and shape. A closely supervised program of splintage and therapy was instituted postoperatively. This series does not vary significantly with respect to range of motion or recurrence of ulnar drift (seen only when patients failed to wear splints as directed) in comparison to other reports. The results, therefore, fail to support the concept that radial capsular and ligamentous reconstruction is an essential part of this procedure.", "contents": "The role of soft tissue reconstruction in flexible implant arthroplasty of the metacarpophalangeal joint. In 116 hand metacarpophalangeal joint arthroplasties of the flexible type, the operative technique did not include reconstruction of capsular or ligamentous structures, but employed meticulous relocation of the common extensor tendons, and a modification of the metacarpal osteotomy plane and shape. A closely supervised program of splintage and therapy was instituted postoperatively. This series does not vary significantly with respect to range of motion or recurrence of ulnar drift (seen only when patients failed to wear splints as directed) in comparison to other reports. The results, therefore, fail to support the concept that radial capsular and ligamentous reconstruction is an essential part of this procedure."}
{"id": "PMID:477074", "title": "A hand infection from A. calcoaceticus (M. polymorpha).", "content": "Acinetobacter calcoaceticus (Mima polymorpha) is not a well known organism, and its identification can be confusing due to its variable morphology and sensitivity pattern. In a joint infection, delay in identification or appropriate treatment can result in added morbidity. An A. calcoaceticus (Mima) infection in the hand seems not to have been previously reported. The present case involving a finger joint infection illustrates the pathogenicity of A. calcoaceticus (Mima) and problems of treatment. Minocycline is recommended as the drug of choice in subsequent A. calcoaceticus (Mima) and (Herella) infections.", "contents": "A hand infection from A. calcoaceticus (M. polymorpha). Acinetobacter calcoaceticus (Mima polymorpha) is not a well known organism, and its identification can be confusing due to its variable morphology and sensitivity pattern. In a joint infection, delay in identification or appropriate treatment can result in added morbidity. An A. calcoaceticus (Mima) infection in the hand seems not to have been previously reported. The present case involving a finger joint infection illustrates the pathogenicity of A. calcoaceticus (Mima) and problems of treatment. Minocycline is recommended as the drug of choice in subsequent A. calcoaceticus (Mima) and (Herella) infections."}
{"id": "PMID:477075", "title": "Superior gluteal artery laceration, a complication of iliac bone graft surgery.", "content": "In the 2 cases cited, laceration of the superior gluteal artery occurred when removing bone from the posterior ilium. A review of the anatomy shows how the superior gluteal artery, as it exists the sciatic notch, can be compressed locally and exposed for clipping or ligation. The internal iliac artery can be occluded by embolization of a Fogerty catheter. The bony origin of the gluteus maximus is a good landmark to help avoid entering the sciatic notch and a special retractor provides the necessary exposure.", "contents": "Superior gluteal artery laceration, a complication of iliac bone graft surgery. In the 2 cases cited, laceration of the superior gluteal artery occurred when removing bone from the posterior ilium. A review of the anatomy shows how the superior gluteal artery, as it exists the sciatic notch, can be compressed locally and exposed for clipping or ligation. The internal iliac artery can be occluded by embolization of a Fogerty catheter. The bony origin of the gluteus maximus is a good landmark to help avoid entering the sciatic notch and a special retractor provides the necessary exposure."}
{"id": "PMID:477076", "title": "Acute bilateral anterior dislocation of the shoulders.", "content": "In 3 cases of simultaneous bilateral anterior dislocation of the shoulders, considering the forces which predispose to anterior dislocations, it is postulated that in each case forced abduction and external rotation or abduction with hyperextension causes these injuries to occur.", "contents": "Acute bilateral anterior dislocation of the shoulders. In 3 cases of simultaneous bilateral anterior dislocation of the shoulders, considering the forces which predispose to anterior dislocations, it is postulated that in each case forced abduction and external rotation or abduction with hyperextension causes these injuries to occur."}
{"id": "PMID:477077", "title": "Calcium requirement and calcium therapy.", "content": "The mean calcium requirement of normal adults has been estimated from 212 calcium balances on 84 normal subjects and found to be 578 mg. The calcium allowance required to ensure that 95% of normal adults are in calcium balance is about 900 mg. Calcium requirement is relatively higher than that of phosphorus or magnesium because plasma calcium--and therefore urine calcium--is maintained at the expense of the skeleton in the presence of calcium deficiency, whereas plasma phosphate and magnesium--and therefore urine phosphate and magnesium--fall on phosphorus and magnesium deficient diets. Calcium requirement appears to rise at the menopause, and postmenopausal bone loss can be reduced by calcium supplements. In postmenopausal osteoporosis, the severe negative calcium balance can be corrected by giving calcium or calcium and vitamin D, but vitamin D alone is not beneficial. Similarly, bone loss, height loss and further vertebral compression can be reduced by calcium or calcium and vitamin D but not by vitamin D alone.", "contents": "Calcium requirement and calcium therapy. The mean calcium requirement of normal adults has been estimated from 212 calcium balances on 84 normal subjects and found to be 578 mg. The calcium allowance required to ensure that 95% of normal adults are in calcium balance is about 900 mg. Calcium requirement is relatively higher than that of phosphorus or magnesium because plasma calcium--and therefore urine calcium--is maintained at the expense of the skeleton in the presence of calcium deficiency, whereas plasma phosphate and magnesium--and therefore urine phosphate and magnesium--fall on phosphorus and magnesium deficient diets. Calcium requirement appears to rise at the menopause, and postmenopausal bone loss can be reduced by calcium supplements. In postmenopausal osteoporosis, the severe negative calcium balance can be corrected by giving calcium or calcium and vitamin D, but vitamin D alone is not beneficial. Similarly, bone loss, height loss and further vertebral compression can be reduced by calcium or calcium and vitamin D but not by vitamin D alone."}
{"id": "PMID:477078", "title": "Compartment syndrome of the interosseous muscles: early recognition and treatment.", "content": "A compartment syndrome of the interosseous muscles can be a challenging diagnosis as there is generally no neurovascular compromise to the digits involved. The most sensitive clinical sign is pain with passive motion at the metacarpal phalangeal joint of the involved digit. In this report, a 31-year-old man developed a compartment syndrome of the first, second, and third dorsal interosseous muscles following an injection of heroin in the \"snuffbox\" area. Compartmental tissue pressure measurements were 80, 75, and 55 mmHg respectively, and were a significant aid in the early diagnosis as well as management.", "contents": "Compartment syndrome of the interosseous muscles: early recognition and treatment. A compartment syndrome of the interosseous muscles can be a challenging diagnosis as there is generally no neurovascular compromise to the digits involved. The most sensitive clinical sign is pain with passive motion at the metacarpal phalangeal joint of the involved digit. In this report, a 31-year-old man developed a compartment syndrome of the first, second, and third dorsal interosseous muscles following an injection of heroin in the \"snuffbox\" area. Compartmental tissue pressure measurements were 80, 75, and 55 mmHg respectively, and were a significant aid in the early diagnosis as well as management."}
{"id": "PMID:477079", "title": "Circulatory and vascular changes in the hip following traumatic hip dislocation.", "content": "An experimental study of the extraosseous and intraosseous vascular and circulatory changes following traumatic hip dislocation in dogs and rabbits is reported. The observations were made by dissection, angiography and histology following posterior dislocation and reduction at varying intervals. Traumatic dislocation of the hip causes extraosseous circulatory disturbance in the hip resulting in intraosseous circulatory deficiency in the femoral head. The sites of the vascular lesions are the extraosseous branches of the femoral circumflex arteries and their extraosseous branches of the epiphyseal and metaphyseal arteries and the retinacular artery of the circulus vasculosus articularis. The types of the vascular damage are irreversible tear of the minority of vessels along the ruptured capsule and teres ligament, and the compression, traction and spasm of the majority of intact vessels which are reversible by early reduction. In prolonged dislocation the circulatory disturbance persists or worsens due to additional pathological changes such as posttraumatic inflammatory changes, fixed deformity in the dislocated position, thrombosis, fibrosis and occlusion of vessels which may eventually cause an ischemic necrosis of the femoral head. Early reduction within several hours after dislocation restores nearly normal regional anatomy and relieves vessels from compression, traction or spasm and restores extra and intraosseous circulation. It also prevents or minimizes thrombosis, fibrosis and occlusion of vessels and avascular necrosis of the femoral head.", "contents": "Circulatory and vascular changes in the hip following traumatic hip dislocation. An experimental study of the extraosseous and intraosseous vascular and circulatory changes following traumatic hip dislocation in dogs and rabbits is reported. The observations were made by dissection, angiography and histology following posterior dislocation and reduction at varying intervals. Traumatic dislocation of the hip causes extraosseous circulatory disturbance in the hip resulting in intraosseous circulatory deficiency in the femoral head. The sites of the vascular lesions are the extraosseous branches of the femoral circumflex arteries and their extraosseous branches of the epiphyseal and metaphyseal arteries and the retinacular artery of the circulus vasculosus articularis. The types of the vascular damage are irreversible tear of the minority of vessels along the ruptured capsule and teres ligament, and the compression, traction and spasm of the majority of intact vessels which are reversible by early reduction. In prolonged dislocation the circulatory disturbance persists or worsens due to additional pathological changes such as posttraumatic inflammatory changes, fixed deformity in the dislocated position, thrombosis, fibrosis and occlusion of vessels which may eventually cause an ischemic necrosis of the femoral head. Early reduction within several hours after dislocation restores nearly normal regional anatomy and relieves vessels from compression, traction or spasm and restores extra and intraosseous circulation. It also prevents or minimizes thrombosis, fibrosis and occlusion of vessels and avascular necrosis of the femoral head."}
{"id": "PMID:477080", "title": "Conservative treatment of low velocity gunshot wounds.", "content": "A retrospective study of gunshot wounds of the extremities present 148 patients who had sufficient clinical and radiological follow-up data to assess the results. The routine treatment for this injury consisted mainly of cleansing the wound, introduction of systemic antibiotics in the emergency room followed by hospitalization. More extensive treatment and surgical debridement were done only when intraarticular or vascular injury occurred. Two patients developed infected wounds. In 6 displaced humeral shaft fractures, there were 2 non-unions and one delayed union. In 11 comminuted, displaced fractures of the femoral shaft, one non-union occurred.", "contents": "Conservative treatment of low velocity gunshot wounds. A retrospective study of gunshot wounds of the extremities present 148 patients who had sufficient clinical and radiological follow-up data to assess the results. The routine treatment for this injury consisted mainly of cleansing the wound, introduction of systemic antibiotics in the emergency room followed by hospitalization. More extensive treatment and surgical debridement were done only when intraarticular or vascular injury occurred. Two patients developed infected wounds. In 6 displaced humeral shaft fractures, there were 2 non-unions and one delayed union. In 11 comminuted, displaced fractures of the femoral shaft, one non-union occurred."}
{"id": "PMID:477082", "title": "Effects of ligamentous injury on ankle and subtalar joints: a kinematic study.", "content": "Using 10 normal fresh cadaveric legs, kinematic effects of ligamentous injuries around the ankle joint were studied while the ankle joint moved from maximum plantar flexion to maximum dorsiflexion in saggittal plane. A series of anteroposterior and lateral sequential roentgenograms was taken both in the normal and subsequently created injured condition, to evaluate instant center of rotation and horizontal rotation pattern in the hindfoot complex. Although the pattern of instant center of rotation of the ankle joint varied among normal condition, the majority fell in a prescribed area. The deviation of instant centers from those in uninjured condition was most evident in lateral ligament injury. The average horizontal rotation between the tibia and calcaneus was 8 degrees in the uninjured condition; 6 degrees in the subtalar joint and 2 degrees in the ankle joint. In both medial and lateral injuries, more rotation in the subtalar joint was noted in plantar flexion phase. In the ankle joint, the degree of excessive rotation in plantar flexion range was greater in lateral injury than medial injury, but that in dorsiflexion range was greater in medial injury. It appeared that while every component of collateral ligaments around the ankle was important in controlling rotation in plantar flexion range, the posterior portion of the deltoid ligament is most important in the forsiflexion range.", "contents": "Effects of ligamentous injury on ankle and subtalar joints: a kinematic study. Using 10 normal fresh cadaveric legs, kinematic effects of ligamentous injuries around the ankle joint were studied while the ankle joint moved from maximum plantar flexion to maximum dorsiflexion in saggittal plane. A series of anteroposterior and lateral sequential roentgenograms was taken both in the normal and subsequently created injured condition, to evaluate instant center of rotation and horizontal rotation pattern in the hindfoot complex. Although the pattern of instant center of rotation of the ankle joint varied among normal condition, the majority fell in a prescribed area. The deviation of instant centers from those in uninjured condition was most evident in lateral ligament injury. The average horizontal rotation between the tibia and calcaneus was 8 degrees in the uninjured condition; 6 degrees in the subtalar joint and 2 degrees in the ankle joint. In both medial and lateral injuries, more rotation in the subtalar joint was noted in plantar flexion phase. In the ankle joint, the degree of excessive rotation in plantar flexion range was greater in lateral injury than medial injury, but that in dorsiflexion range was greater in medial injury. It appeared that while every component of collateral ligaments around the ankle was important in controlling rotation in plantar flexion range, the posterior portion of the deltoid ligament is most important in the forsiflexion range."}
{"id": "PMID:477081", "title": "Experimental knee instability: early mechanical property changes in articular cartilage in a rabbit model.", "content": "Utilizing a knee joint instability model for traumatic osteoarthritis, quantitative measurements of the biomechanical properties of the articular cartilage demonstrate that the cartilage deforms and that the deformation rapidly occurs within 24 hours of injury. These changes in mechanical properties may be a more sensitive indicator of early degenerative changes in articular cartilage than the ordinary histological measurements.", "contents": "Experimental knee instability: early mechanical property changes in articular cartilage in a rabbit model. Utilizing a knee joint instability model for traumatic osteoarthritis, quantitative measurements of the biomechanical properties of the articular cartilage demonstrate that the cartilage deforms and that the deformation rapidly occurs within 24 hours of injury. These changes in mechanical properties may be a more sensitive indicator of early degenerative changes in articular cartilage than the ordinary histological measurements."}
{"id": "PMID:477085", "title": "Paramedian neuroma presenting as a ganglion.", "content": "Peripheral nerve tumors are rare, usually encapsulated and intraneural in location. A 33-year-old woman with a nonencapsulated paraneural neoplasm of the median nerve obtained pain relief without recurrence from surgical excision of the tumor. This represents an exceedingly rare disorder of peripheral nerves.", "contents": "Paramedian neuroma presenting as a ganglion. Peripheral nerve tumors are rare, usually encapsulated and intraneural in location. A 33-year-old woman with a nonencapsulated paraneural neoplasm of the median nerve obtained pain relief without recurrence from surgical excision of the tumor. This represents an exceedingly rare disorder of peripheral nerves."}
{"id": "PMID:477086", "title": "A quantitative sutdy of vertebral displacement and angulation in the normal cervical spine under axial load.", "content": "Cervical traction of one-third the person's weight was applied for 3 minutes to normal subjects. The mean and standard deviation of displacement and rotation between adjacent cervical vertebra was calculated in the sagittal plane. The average separation between adjacent vertebra was 0.7 mm with a standard deviation of 0.5 mm. The average angular motion between adjacent vertebra was 0.9 degrees with a standard deviation of 2.4 degrees.", "contents": "A quantitative sutdy of vertebral displacement and angulation in the normal cervical spine under axial load. Cervical traction of one-third the person's weight was applied for 3 minutes to normal subjects. The mean and standard deviation of displacement and rotation between adjacent cervical vertebra was calculated in the sagittal plane. The average separation between adjacent vertebra was 0.7 mm with a standard deviation of 0.5 mm. The average angular motion between adjacent vertebra was 0.9 degrees with a standard deviation of 2.4 degrees."}
{"id": "PMID:477087", "title": "Rattlesnake bites: current concepts.", "content": "The management of snake bites once the patient reaches the hospital should be to obtain the necessary blood parameters (type and cross-matched blood, complete blood count, platelets, PT, PTT, fibrinogen and fibrin split products, electrolytes and calcium), to evaluate the fang marks and the neurovascular status of the involved extremity and to monitor systemic signs and symptoms. These steps are extensively described in the literature, and are commonly agreed upon. We then start intravenous antivenom, after appropriate skin testing for horse serum allergy, beginning with 4--5 vials (8--10 in children) over the initial 30--60 minutes and titrating to cessation of the progression of the toxic signs. Concomitantly, we measure intracompartmental and subcutaneous tissue pressure in all clinically suspected areas. If intracompartment pressures are less than 30 mm Hg, surgical intervention is not necessary; antivenom is continued as necessary and the wick catheter measurements are repeated if indicated. If pressures are greater than 30 mm Hg,immediate surgical decompression is advisable.", "contents": "Rattlesnake bites: current concepts. The management of snake bites once the patient reaches the hospital should be to obtain the necessary blood parameters (type and cross-matched blood, complete blood count, platelets, PT, PTT, fibrinogen and fibrin split products, electrolytes and calcium), to evaluate the fang marks and the neurovascular status of the involved extremity and to monitor systemic signs and symptoms. These steps are extensively described in the literature, and are commonly agreed upon. We then start intravenous antivenom, after appropriate skin testing for horse serum allergy, beginning with 4--5 vials (8--10 in children) over the initial 30--60 minutes and titrating to cessation of the progression of the toxic signs. Concomitantly, we measure intracompartmental and subcutaneous tissue pressure in all clinically suspected areas. If intracompartment pressures are less than 30 mm Hg, surgical intervention is not necessary; antivenom is continued as necessary and the wick catheter measurements are repeated if indicated. If pressures are greater than 30 mm Hg,immediate surgical decompression is advisable."}
{"id": "PMID:477088", "title": "Coccidioidomycosis of the spine: unusual roentgenographic presentation.", "content": "Vertebral osteomyelitis is a frequent consequence of disseminated coccidioidomycosis. In a 25-year-old man, the sequela was a single level unilateral osseous bridge between the second and third lumbar vertebra. A review of previous cases has disclosed one case with a similar roentgenographic picture. The occurrence of a single level unilateral reactive process should arouse suspicion of a localized chronic bone infection.", "contents": "Coccidioidomycosis of the spine: unusual roentgenographic presentation. Vertebral osteomyelitis is a frequent consequence of disseminated coccidioidomycosis. In a 25-year-old man, the sequela was a single level unilateral osseous bridge between the second and third lumbar vertebra. A review of previous cases has disclosed one case with a similar roentgenographic picture. The occurrence of a single level unilateral reactive process should arouse suspicion of a localized chronic bone infection."}
{"id": "PMID:477090", "title": "The spike procedure of Mumford: a simple technique is described for stabilizing open fractures of tubular bones.", "content": "The spike procedure is applicable as a substitute for a fixation device. In open tubular bone fractures, the spike procedure utilizes the open fracture bone ends to create an intrinsically stable situation in place of pins or extrinsic devices. One end of the tubular bone fracture is fitted into the medullary canal of the other end.", "contents": "The spike procedure of Mumford: a simple technique is described for stabilizing open fractures of tubular bones. The spike procedure is applicable as a substitute for a fixation device. In open tubular bone fractures, the spike procedure utilizes the open fracture bone ends to create an intrinsically stable situation in place of pins or extrinsic devices. One end of the tubular bone fracture is fitted into the medullary canal of the other end."}
{"id": "PMID:477089", "title": "A histochemical study of alkaline and acid phosphatase activity in subchondral bone from osteoarthrotic human hips.", "content": "In order to analyze the presence of alkaline and acid phosphatase activity in osteoarthrotic subchondral bone frozen sections from 24 femoral heads were prepared for semiquantitative analysis, using the enzyme-histochemical methods described by Burstone and by Barka and Anderson. Different areas of the subchondral bone, viz. weight-bearing, nonweight-bearing and osteophytes as well as central regions were analyzed. Furthermore, the cartilagenous changes were determined by histological-histochemical grading. There were wide variations within the same femoral head with significantly greater activity of both alkaline and acid phosphatase in weight-bearing than in nonweight-bearing areas and in subchondral bone than in central regions. Osteophytes excluded, the enzyme activities correlated directly with the severity of the cartilage lesions. These enzyme activities presumably reflect the degree of bone regeneration and bone resorption respectively.", "contents": "A histochemical study of alkaline and acid phosphatase activity in subchondral bone from osteoarthrotic human hips. In order to analyze the presence of alkaline and acid phosphatase activity in osteoarthrotic subchondral bone frozen sections from 24 femoral heads were prepared for semiquantitative analysis, using the enzyme-histochemical methods described by Burstone and by Barka and Anderson. Different areas of the subchondral bone, viz. weight-bearing, nonweight-bearing and osteophytes as well as central regions were analyzed. Furthermore, the cartilagenous changes were determined by histological-histochemical grading. There were wide variations within the same femoral head with significantly greater activity of both alkaline and acid phosphatase in weight-bearing than in nonweight-bearing areas and in subchondral bone than in central regions. Osteophytes excluded, the enzyme activities correlated directly with the severity of the cartilage lesions. These enzyme activities presumably reflect the degree of bone regeneration and bone resorption respectively."}
{"id": "PMID:477092", "title": "Mechanical stability of the greater trochanter following osteotomy and reattachment by wiring.", "content": "Greater trochanters of fresh and formalin-perserved cadaver femora were osteotomized, rewired using techniques described by Amstutz, Charnley, Coventry and Harris, and then mechanically tested. Force simulating abductor muscle pull was applied to the trochanter in the plane of section while its displacement was measured continuously with an extensometer. Although many trochanters appeared rigidly fixed when stressed manually, all displaced visibly when 27 kg load was first applied. When released, permanent sets averaging 0.1 mm (Charnley and Harris) to 3--4 mm (Coventry) were measured. Subsequent repeated loadings (0--27 kg) from this new \"rest\" position produced recoverable cyclic \"functional\" displacements averaging 0.7 mm (Charnley and Harris) to 2.3 mm (Coventry). With the exception of the specimens wired by the Coventry technique, results for a simulated 45 degrees anterior pull were generally comparable to those for a neutral pull. Ultimate failure loads of the trochanteric fixation were on the order of 110 kg for selected fresh specimens.", "contents": "Mechanical stability of the greater trochanter following osteotomy and reattachment by wiring. Greater trochanters of fresh and formalin-perserved cadaver femora were osteotomized, rewired using techniques described by Amstutz, Charnley, Coventry and Harris, and then mechanically tested. Force simulating abductor muscle pull was applied to the trochanter in the plane of section while its displacement was measured continuously with an extensometer. Although many trochanters appeared rigidly fixed when stressed manually, all displaced visibly when 27 kg load was first applied. When released, permanent sets averaging 0.1 mm (Charnley and Harris) to 3--4 mm (Coventry) were measured. Subsequent repeated loadings (0--27 kg) from this new \"rest\" position produced recoverable cyclic \"functional\" displacements averaging 0.7 mm (Charnley and Harris) to 2.3 mm (Coventry). With the exception of the specimens wired by the Coventry technique, results for a simulated 45 degrees anterior pull were generally comparable to those for a neutral pull. Ultimate failure loads of the trochanteric fixation were on the order of 110 kg for selected fresh specimens."}
{"id": "PMID:477093", "title": "The mechanisms of severe arterial injury in surgery of the hip joint.", "content": "Vascular accidents occurring in the course of hip surgery may reach potentially catastrophic dimensions by posing an immediate and sudden threat to life and limb. This is a report of 15 cases with severe arterial injury representing 0.2--0.3% of all reconstructive hip operations performed during an 8 year period. In 6 cases perforation of either the external iliac artery, the common femoral artery of main branches of the lateral and medial circumflex femoral artery were caused by the tip of a narrow-pointed Hohmann retractor used to expose the hip joint. Other mechanisms were: intimal tear with appositional thrombosis, probably caused by mechanical strain imposed on atherosclerotic arteries, giving rise to complete limb ischemia (2 cases); the dangers associated with the entry of bone cement through a defective acetabulum into the pelvis causing thrombotic occlusion due to polymerization heat (one case) or intimate adhesion of artificial bone to the external iliac artery subsequently being ripped open during replacement of the cup (one case); the increased hazards of replacing firmly embedded hip prosthesis (3 cases of direct arterial injury with chisel, knife and cutting edge of protruding bone); and the complications associated with the development of a false aneurysm (2 cases). Fourteen of the 15 extremities were salvaged. Above-knee amputation was unavoidable in one case owing to delay of vascular repair. There was no immediate operative mortality. Knowledge of the causative mechanisms prevents arterial injury during hip surgery. The relatively low rate of vascular complications in spite of vicinity of main vessels gives credit to the well standardized technique of hip surgery, especially hip replacement. However, it is suggested that the surgeon should be sufficiently acquainted with the exposure of the main vessels above and below the groin to be able to control life threatening hemorrhage at all times. A McBurney incision with retroperitoneal exposure and clamping of the external iliac artery will suffice to diminish bleeding considerably. Thereupon careful dissection and placement of snares around the common femoral artery, the arteria profunda femoris, and whenever necessary, the lateral or medial circumflex femoral artery will enable closure of the lacerated artery. For hemorrhage resulting during replacement of firmly embedded hip prosthesis it might become necessary to ligate the internal iliac artery. Reconstruction of obliterated arteries should call for the cooperation of the vascular surgeon for eventual angioplasty. Angiologic examination of the lower extremities is mandatory whenever severe arterial trauma has occurred in the course of hip surgery and is best performed by measuring the ankle blood pressure with a Doppler ultrasound probe.", "contents": "The mechanisms of severe arterial injury in surgery of the hip joint. Vascular accidents occurring in the course of hip surgery may reach potentially catastrophic dimensions by posing an immediate and sudden threat to life and limb. This is a report of 15 cases with severe arterial injury representing 0.2--0.3% of all reconstructive hip operations performed during an 8 year period. In 6 cases perforation of either the external iliac artery, the common femoral artery of main branches of the lateral and medial circumflex femoral artery were caused by the tip of a narrow-pointed Hohmann retractor used to expose the hip joint. Other mechanisms were: intimal tear with appositional thrombosis, probably caused by mechanical strain imposed on atherosclerotic arteries, giving rise to complete limb ischemia (2 cases); the dangers associated with the entry of bone cement through a defective acetabulum into the pelvis causing thrombotic occlusion due to polymerization heat (one case) or intimate adhesion of artificial bone to the external iliac artery subsequently being ripped open during replacement of the cup (one case); the increased hazards of replacing firmly embedded hip prosthesis (3 cases of direct arterial injury with chisel, knife and cutting edge of protruding bone); and the complications associated with the development of a false aneurysm (2 cases). Fourteen of the 15 extremities were salvaged. Above-knee amputation was unavoidable in one case owing to delay of vascular repair. There was no immediate operative mortality. Knowledge of the causative mechanisms prevents arterial injury during hip surgery. The relatively low rate of vascular complications in spite of vicinity of main vessels gives credit to the well standardized technique of hip surgery, especially hip replacement. However, it is suggested that the surgeon should be sufficiently acquainted with the exposure of the main vessels above and below the groin to be able to control life threatening hemorrhage at all times. A McBurney incision with retroperitoneal exposure and clamping of the external iliac artery will suffice to diminish bleeding considerably. Thereupon careful dissection and placement of snares around the common femoral artery, the arteria profunda femoris, and whenever necessary, the lateral or medial circumflex femoral artery will enable closure of the lacerated artery. For hemorrhage resulting during replacement of firmly embedded hip prosthesis it might become necessary to ligate the internal iliac artery. Reconstruction of obliterated arteries should call for the cooperation of the vascular surgeon for eventual angioplasty. Angiologic examination of the lower extremities is mandatory whenever severe arterial trauma has occurred in the course of hip surgery and is best performed by measuring the ankle blood pressure with a Doppler ultrasound probe."}
{"id": "PMID:477094", "title": "The efficacy of low-dose heparin--warfarin anticoagulation prophylaxis after total hip replacement arthroplasty.", "content": "The prime therapeutic objective of prophylactic anticoagulation for patients undergoing total hip replacement is to reduce to a minimum fatalities from pulmonary embolism. Our low-dose heparin-warfarin prophylactic anticoagulation protocol affords significant protection in this regard (one fatal pulmonary embolism in 796 cases) without the use of venography or other objective tests to check for deep venous thrombosis and for all patients including those with venous disease or a history of prior thromboembolic disease. The 13.1% hematoma rate (5.0% late major) is a small, nonlethal, and acceptable price to pay for this protection.", "contents": "The efficacy of low-dose heparin--warfarin anticoagulation prophylaxis after total hip replacement arthroplasty. The prime therapeutic objective of prophylactic anticoagulation for patients undergoing total hip replacement is to reduce to a minimum fatalities from pulmonary embolism. Our low-dose heparin-warfarin prophylactic anticoagulation protocol affords significant protection in this regard (one fatal pulmonary embolism in 796 cases) without the use of venography or other objective tests to check for deep venous thrombosis and for all patients including those with venous disease or a history of prior thromboembolic disease. The 13.1% hematoma rate (5.0% late major) is a small, nonlethal, and acceptable price to pay for this protection."}
{"id": "PMID:477095", "title": "Total hip replacement following infection in the opposite hip.", "content": "Total hip replacements without ensuing infection were performed in 31 patients in the presence of infection in the contralateral hip. Infection in the opposite hip was proved bacteriologically in the follow-up in 19 cases averaged 5 years (2 years 3 months to 10 years). In another 12 hips in the same circumstances, without positive bacteriological evidence of active infection on the opposite side, healing occurred without infection.", "contents": "Total hip replacement following infection in the opposite hip. Total hip replacements without ensuing infection were performed in 31 patients in the presence of infection in the contralateral hip. Infection in the opposite hip was proved bacteriologically in the follow-up in 19 cases averaged 5 years (2 years 3 months to 10 years). In another 12 hips in the same circumstances, without positive bacteriological evidence of active infection on the opposite side, healing occurred without infection."}
{"id": "PMID:477097", "title": "Femoral shaft perforation: a complication of total hip reconstruction.", "content": "Perforation of the femoral shaft by the stem of the prosthesis is a rare, but serious complication following total hip replacement. It is a complication that is avoidable if meticulous attention is given to preoperative planning and intraoperative technique. The major predisposing factors for this complication are previous hip surgery, hip dysplasia, osteoporosis and poor exposure. Every attempt should be made to discover a perforation during surgery and to correct the alignment of the femoral stem. The patients who have experienced this complication have had a poor result suffering pain, loosening or femoral shaft fracture necessitating surgical revision.", "contents": "Femoral shaft perforation: a complication of total hip reconstruction. Perforation of the femoral shaft by the stem of the prosthesis is a rare, but serious complication following total hip replacement. It is a complication that is avoidable if meticulous attention is given to preoperative planning and intraoperative technique. The major predisposing factors for this complication are previous hip surgery, hip dysplasia, osteoporosis and poor exposure. Every attempt should be made to discover a perforation during surgery and to correct the alignment of the femoral stem. The patients who have experienced this complication have had a poor result suffering pain, loosening or femoral shaft fracture necessitating surgical revision."}
{"id": "PMID:477096", "title": "Treatment of subacute sepsis of the hip by antibiotics and joint replacement. Criteria for diagnosis with evaluation of twenty-six cases.", "content": "Total hip replacement fixed with acrylic cement can be successful despite past or present hip infection. Recovery of the infecting organism must be vigorously pursued, either by repeated aspirations or open biopsies. Staging the reconstruction is determined by the pathogenicity of the infecting organism, gram stain, frozen section, and above all the surgeon's observations of the condition of the wound. If in doubt, treatment of infection must take precedence over the reconstruction. Adequate antibiotic coverage should begin as soon as the diagnosis is certain and the organism identified. Antibiotics should be continued postoperatively for one month intravenously, followed by 4--8 months oral therapy, though the length of oral antibiotics could be debated. A number of authors have based their diagnosis of hip infection on clinical, roentgenographic, laboratory and bacteriologic studies. However, they have not given specific guidelines for the determination of deep hip infection. The application of the criteria outlined in this paper can distinguish superficial from deep hip infection as illustrated in 40 cases with no evidence of recurrence of infection. For the present only relative guidelines may be drawn for the treatment of subacute or recently arrested sepsis of the hip. Considering some 80,000 total hip replacements done in the U.S. in the past year, a significant number of patients will require treatment of this type and appropriate programs should be established for the welfare of the patients.", "contents": "Treatment of subacute sepsis of the hip by antibiotics and joint replacement. Criteria for diagnosis with evaluation of twenty-six cases. Total hip replacement fixed with acrylic cement can be successful despite past or present hip infection. Recovery of the infecting organism must be vigorously pursued, either by repeated aspirations or open biopsies. Staging the reconstruction is determined by the pathogenicity of the infecting organism, gram stain, frozen section, and above all the surgeon's observations of the condition of the wound. If in doubt, treatment of infection must take precedence over the reconstruction. Adequate antibiotic coverage should begin as soon as the diagnosis is certain and the organism identified. Antibiotics should be continued postoperatively for one month intravenously, followed by 4--8 months oral therapy, though the length of oral antibiotics could be debated. A number of authors have based their diagnosis of hip infection on clinical, roentgenographic, laboratory and bacteriologic studies. However, they have not given specific guidelines for the determination of deep hip infection. The application of the criteria outlined in this paper can distinguish superficial from deep hip infection as illustrated in 40 cases with no evidence of recurrence of infection. For the present only relative guidelines may be drawn for the treatment of subacute or recently arrested sepsis of the hip. Considering some 80,000 total hip replacements done in the U.S. in the past year, a significant number of patients will require treatment of this type and appropriate programs should be established for the welfare of the patients."}
{"id": "PMID:477091", "title": "Trochanteric osteotomy: analysis of pattern of wire fixation failure and complications.", "content": "Problems and complications following trochanteric osteotomy in 277 total hip replacements demonstrated that while the value of trochanteric osteotomy is obvious, the incidence of problems and complications in 277 total hip replacements was about 15%. Analysis was made of the location and sequence of wire failure following different techniques of trochanteric wiring. The usual method of wire failure was by fatigue fracture, although early fracture in the post-operative phase may be secondary to tensile failure, The incidence of wire tensile failure can be increased by inadvertent kinking or scoring of the wire during trochanteric fixation. An ununited trochanter should be protected against wire failure and its sequelae.", "contents": "Trochanteric osteotomy: analysis of pattern of wire fixation failure and complications. Problems and complications following trochanteric osteotomy in 277 total hip replacements demonstrated that while the value of trochanteric osteotomy is obvious, the incidence of problems and complications in 277 total hip replacements was about 15%. Analysis was made of the location and sequence of wire failure following different techniques of trochanteric wiring. The usual method of wire failure was by fatigue fracture, although early fracture in the post-operative phase may be secondary to tensile failure, The incidence of wire tensile failure can be increased by inadvertent kinking or scoring of the wire during trochanteric fixation. An ununited trochanter should be protected against wire failure and its sequelae."}
{"id": "PMID:477098", "title": "Precise identification of radiographic acetabular landmarks.", "content": "Accurate radiographic demonstration of pelvic anatomical landmarks is predicated on proper positioning of the patient. Interpretation of acetabular abnormality especially may be difficult when to the artifact created by pelvic asymmetrical positioning there is added true femoral head displacement. A radiograph of hip joint abnormalities in a child with congenital hip dislocation where positional distortion was present served to illustrate the problem. To clarify the direction and magnitude of acetabluar alterations with specific pelvic positions, radiographs and photographs were taken of anatomic models outlined by suitably placed lead markers. Clues to inappropriate pelvic positioning are loss of symmetry in the widths of the iliac alae, sizes of the obturator foramina, and positions of the \"tear drop\" figures.", "contents": "Precise identification of radiographic acetabular landmarks. Accurate radiographic demonstration of pelvic anatomical landmarks is predicated on proper positioning of the patient. Interpretation of acetabular abnormality especially may be difficult when to the artifact created by pelvic asymmetrical positioning there is added true femoral head displacement. A radiograph of hip joint abnormalities in a child with congenital hip dislocation where positional distortion was present served to illustrate the problem. To clarify the direction and magnitude of acetabluar alterations with specific pelvic positions, radiographs and photographs were taken of anatomic models outlined by suitably placed lead markers. Clues to inappropriate pelvic positioning are loss of symmetry in the widths of the iliac alae, sizes of the obturator foramina, and positions of the \"tear drop\" figures."}
{"id": "PMID:477099", "title": "The Giliberty bipolar prosthesis: a clinical and radiographical review.", "content": "An interim report of 65 Giliberty bipolar hip units followed for an average of 19 months shows excellent short-term results in regard to their clinical condition. Radiographic study of the prosthesis with special attention to cup movement portends a favorable long-term result. Nevertheless, the snap-fit mechanism of the prosthetic head in the cup and the moving cup justify a cautious attitude until a larger series of a longer term can be completed.", "contents": "The Giliberty bipolar prosthesis: a clinical and radiographical review. An interim report of 65 Giliberty bipolar hip units followed for an average of 19 months shows excellent short-term results in regard to their clinical condition. Radiographic study of the prosthesis with special attention to cup movement portends a favorable long-term result. Nevertheless, the snap-fit mechanism of the prosthetic head in the cup and the moving cup justify a cautious attitude until a larger series of a longer term can be completed."}
{"id": "PMID:477100", "title": "\"Modes of failure\" of cemented stem-type femoral components: a radiographic analysis of loosening.", "content": "In view of the increasing incidence of stem-type femoral component loosening, a detailed retrospective radiographic zonal analysis of 389 total hip replacements indicated a 19.5% incidence (76 hips) of radiological evidences of mechanical looseness, i.e., fractured acrylic cement and/or a radiolucent gap at the stem-cement or cement-bone interfaces. Detailed serial radiographic examination demonstrated progressive loosening in 56 of the 76 hips and these were categorized into mechanical modes of failure. The 4 modes of failure characterizing stem-type component progressive loosening mechanisms consisted of stem pistoning within the acrylic (3.3%), cement-embedded stem pistoning with the femur (5.1%), medial midstem pivot (2.5%), calcar pivot (0.7%) and bending (fatigue) cantilever (3.3%).", "contents": "\"Modes of failure\" of cemented stem-type femoral components: a radiographic analysis of loosening. In view of the increasing incidence of stem-type femoral component loosening, a detailed retrospective radiographic zonal analysis of 389 total hip replacements indicated a 19.5% incidence (76 hips) of radiological evidences of mechanical looseness, i.e., fractured acrylic cement and/or a radiolucent gap at the stem-cement or cement-bone interfaces. Detailed serial radiographic examination demonstrated progressive loosening in 56 of the 76 hips and these were categorized into mechanical modes of failure. The 4 modes of failure characterizing stem-type component progressive loosening mechanisms consisted of stem pistoning within the acrylic (3.3%), cement-embedded stem pistoning with the femur (5.1%), medial midstem pivot (2.5%), calcar pivot (0.7%) and bending (fatigue) cantilever (3.3%)."}
{"id": "PMID:477101", "title": "Slipped capital femoral epiphysis long-term results after 10--38 years.", "content": "Thirty-four patients with chronic slipped capital femoral epiphysis (SCFE) involving 45 hips were followed over a period of no less than 10 years and as great as 38 years. Excluding those who had had intraoperative complications, 30 hips evaluated between 10 and 20 years follow-up were found to have good or excellent results. Fifteen hips followed greater than 20 years, however, showed fair to poor grading in 10 of 15 hips. This progessive change seemed to be related most closely to the bilateralism of the disease. These observations suggest that while follow-up of a 20 year duration may show good clinical results, over a longer period of time the prognosis of SCFE is guarded.", "contents": "Slipped capital femoral epiphysis long-term results after 10--38 years. Thirty-four patients with chronic slipped capital femoral epiphysis (SCFE) involving 45 hips were followed over a period of no less than 10 years and as great as 38 years. Excluding those who had had intraoperative complications, 30 hips evaluated between 10 and 20 years follow-up were found to have good or excellent results. Fifteen hips followed greater than 20 years, however, showed fair to poor grading in 10 of 15 hips. This progessive change seemed to be related most closely to the bilateralism of the disease. These observations suggest that while follow-up of a 20 year duration may show good clinical results, over a longer period of time the prognosis of SCFE is guarded."}
{"id": "PMID:477103", "title": "Intertrochanteric fractures of the hip treated with the hip compression screw: analysis of problems.", "content": "Seventy-five patients were treated for intertrochanteric hip fractures with the hip compression screw. There were 4 cases in which the lag screw was inserted twice into the femoral head. Three of these patients had a poor result due to superolateral migration and extrusion of the lag screw. The fact that the lag screw is large in comparison to the femoral head makes double placement dangerous. Satisfactory guide wire placement is essential for a one time, precise insertion of the lag screw into the femoral head. The use of a threaded tip guide wire minimizes the chance of it falling out when withdrawing the reamer or tap. If the position of a lag screw is unacceptable, it seems better to insert a flanged nail rather than a second screw in a second track.", "contents": "Intertrochanteric fractures of the hip treated with the hip compression screw: analysis of problems. Seventy-five patients were treated for intertrochanteric hip fractures with the hip compression screw. There were 4 cases in which the lag screw was inserted twice into the femoral head. Three of these patients had a poor result due to superolateral migration and extrusion of the lag screw. The fact that the lag screw is large in comparison to the femoral head makes double placement dangerous. Satisfactory guide wire placement is essential for a one time, precise insertion of the lag screw into the femoral head. The use of a threaded tip guide wire minimizes the chance of it falling out when withdrawing the reamer or tap. If the position of a lag screw is unacceptable, it seems better to insert a flanged nail rather than a second screw in a second track."}
{"id": "PMID:477104", "title": "Intertrochanteric fractures of the hip in the elderly: a retrospective analysis of 236 cases.", "content": "A series of 236 patients over the age of 80 with intertrochanteric fractures were treated on a protocol established basis in an attempt to decrease postoperative morbidity and mortality. Rigid fixation with interfragmentary compression using the compression hip screw permitted us to begin early mobilization and immediate weight-bearing. Vigorous pulmonary tiolette, prophylactic antibiotics, and aspirin \"anticoagulation\" were used as adjuncts. Three month mortality was 7%. Overall, 76% of the patients who ambulated in any manner before their fracture walked again after surgery. Many, however, lost one grade level of walking postoperatively. Effective treatment for a patient with an intertrochanteric fracture, regardless of advanced age, is surgery and early mobilization.", "contents": "Intertrochanteric fractures of the hip in the elderly: a retrospective analysis of 236 cases. A series of 236 patients over the age of 80 with intertrochanteric fractures were treated on a protocol established basis in an attempt to decrease postoperative morbidity and mortality. Rigid fixation with interfragmentary compression using the compression hip screw permitted us to begin early mobilization and immediate weight-bearing. Vigorous pulmonary tiolette, prophylactic antibiotics, and aspirin \"anticoagulation\" were used as adjuncts. Three month mortality was 7%. Overall, 76% of the patients who ambulated in any manner before their fracture walked again after surgery. Many, however, lost one grade level of walking postoperatively. Effective treatment for a patient with an intertrochanteric fracture, regardless of advanced age, is surgery and early mobilization."}
{"id": "PMID:477106", "title": "Clinical study on internal fixation of femoral neck fractures.", "content": "A clinical study on the results of internal fixation of femoral neck fractures treated by multiple Knowles pinning was undertaken and correlated with the results of an experimental study. Experimentally, failures were produced by downwards migration of the Knowles pins. This phenomenon accounts for the settling of the femoral head on the neck and the frequency of non-union or malunion in patients with subcapital fractures. The type of fracture, type of reduction and age correlate with failure rate. This has to be explained by biomechanical considerations based upon the mode of failure of the internal fixation. A Garden stage III and IV, a varus or anatomical reduction of the fracture and an old age predispose to failure of the internal fixation and consequent non-union.", "contents": "Clinical study on internal fixation of femoral neck fractures. A clinical study on the results of internal fixation of femoral neck fractures treated by multiple Knowles pinning was undertaken and correlated with the results of an experimental study. Experimentally, failures were produced by downwards migration of the Knowles pins. This phenomenon accounts for the settling of the femoral head on the neck and the frequency of non-union or malunion in patients with subcapital fractures. The type of fracture, type of reduction and age correlate with failure rate. This has to be explained by biomechanical considerations based upon the mode of failure of the internal fixation. A Garden stage III and IV, a varus or anatomical reduction of the fracture and an old age predispose to failure of the internal fixation and consequent non-union."}
{"id": "PMID:477105", "title": "Internal fixation versus nontreatment of hip fractures in senile patients.", "content": "Institutionalized senile patients with hip fractures have a high mortality regardless of whether treatment consists of internal stabilization or is non-operative. In an effort to select the best therapeutic approach in this type of patient, 150 patients were examined in 3 comparable groups. In the first group of 50 patients treatment consisted of surgical stabilization of the fracture. In the second group of 45 patients treatment was non-operative; mortality in these 2 groups was over 50% at the end of 16 weeks. In an effort to improve this degree of risk we transferred 53 patients from the psychiatric hospital to a general hospital for prompt internal stabilization of the hip fractures. There was a striking decrease in mortality to 28% in the third group. We have concluded that under optimal conditions, prompt surgical stabilization of hip fractures in elderly senile individuals offers them the best opportunity of surviving the injury and is preferable to nontreatment.", "contents": "Internal fixation versus nontreatment of hip fractures in senile patients. Institutionalized senile patients with hip fractures have a high mortality regardless of whether treatment consists of internal stabilization or is non-operative. In an effort to select the best therapeutic approach in this type of patient, 150 patients were examined in 3 comparable groups. In the first group of 50 patients treatment consisted of surgical stabilization of the fracture. In the second group of 45 patients treatment was non-operative; mortality in these 2 groups was over 50% at the end of 16 weeks. In an effort to improve this degree of risk we transferred 53 patients from the psychiatric hospital to a general hospital for prompt internal stabilization of the hip fractures. There was a striking decrease in mortality to 28% in the third group. We have concluded that under optimal conditions, prompt surgical stabilization of hip fractures in elderly senile individuals offers them the best opportunity of surviving the injury and is preferable to nontreatment."}
{"id": "PMID:477107", "title": "An uncemented total hip replacement: experimental study and review of 300 madreporique arthroplasties.", "content": "Mafreporique arthroplasty is not intended as a bustitute for acrylic cement stabilized arthroplasty but is available as an effective alternative. An experimental and clinical investigation including radiological analysis of 300 madreporique arthroplasties has convinced us of the reality and reliability of biological anchoring by endosteal osteogenesis. The madreporique method does not result in any local or general complications usually associated with the cement technique. Full weight-bearing is permitted at the same postoperative time as the cemented prosthesis. The results at early and interim follow-up periods compare favorably with cemented hip arthroplasties. Living bone that undergoes remodelling provides for long-term anchor of the prosthesis and eliminates the chemical intermediary which leads, more or less, with time to loosening of the implant in a significant number of cases.", "contents": "An uncemented total hip replacement: experimental study and review of 300 madreporique arthroplasties. Mafreporique arthroplasty is not intended as a bustitute for acrylic cement stabilized arthroplasty but is available as an effective alternative. An experimental and clinical investigation including radiological analysis of 300 madreporique arthroplasties has convinced us of the reality and reliability of biological anchoring by endosteal osteogenesis. The madreporique method does not result in any local or general complications usually associated with the cement technique. Full weight-bearing is permitted at the same postoperative time as the cemented prosthesis. The results at early and interim follow-up periods compare favorably with cemented hip arthroplasties. Living bone that undergoes remodelling provides for long-term anchor of the prosthesis and eliminates the chemical intermediary which leads, more or less, with time to loosening of the implant in a significant number of cases."}
{"id": "PMID:477109", "title": "Experimental study on internal fixation of femoral neck fractures.", "content": "The relative strength and the failure mode of internal fixation by multiple pinning and nailing was investigated on 35 specimens. Internal fixation consisted of 3, 5 and 7 Knowles pins, a Smith-Petersen nail or a sliding nail plate. The strength of a specimen with a triflanged nail is only half of that with multiple Knowles pins. There is no significant difference in strength of 3, 5, or 7 pin specimens. The strength of an internal fixation with a sliding nail plate is not superior to the strength of multiple pinning. The mode of failure for the different types of internal fixation is primarily a crush fracture of the supporting trabecular bone at the femoral neck with downward and outward migration of the pins or nail. Except with the fixation with 3 pins where an initial bending could be observed, failure is not a plastic deformation of the internal fixation device.", "contents": "Experimental study on internal fixation of femoral neck fractures. The relative strength and the failure mode of internal fixation by multiple pinning and nailing was investigated on 35 specimens. Internal fixation consisted of 3, 5 and 7 Knowles pins, a Smith-Petersen nail or a sliding nail plate. The strength of a specimen with a triflanged nail is only half of that with multiple Knowles pins. There is no significant difference in strength of 3, 5, or 7 pin specimens. The strength of an internal fixation with a sliding nail plate is not superior to the strength of multiple pinning. The mode of failure for the different types of internal fixation is primarily a crush fracture of the supporting trabecular bone at the femoral neck with downward and outward migration of the pins or nail. Except with the fixation with 3 pins where an initial bending could be observed, failure is not a plastic deformation of the internal fixation device."}
{"id": "PMID:477110", "title": "A review of fractures in the proximal femur treated with the Zickel nail.", "content": "The Zickel intramedullary fixation device was used to treat 26 patients with subtrochanteric or impending subtrochanteric fractures. The use of this device has advantages over traditional traction methods as well as nail plate fixation. Twenty-two patients were ambulatory within one month following surgery. Healing of the fracture by roentenogram required an average of 4.9 months and had a high union rate, 17 out of 18 patients was apparent with the use of the Zickel device. There is no classification of fractures at present which has a prognostic value when intramedullary fixation is used. Traumatic and pathologic fractures were treated with similar results. There was no appliance failure in this series.", "contents": "A review of fractures in the proximal femur treated with the Zickel nail. The Zickel intramedullary fixation device was used to treat 26 patients with subtrochanteric or impending subtrochanteric fractures. The use of this device has advantages over traditional traction methods as well as nail plate fixation. Twenty-two patients were ambulatory within one month following surgery. Healing of the fracture by roentenogram required an average of 4.9 months and had a high union rate, 17 out of 18 patients was apparent with the use of the Zickel device. There is no classification of fractures at present which has a prognostic value when intramedullary fixation is used. Traumatic and pathologic fractures were treated with similar results. There was no appliance failure in this series."}
{"id": "PMID:477111", "title": "Hip disease of Mseleni.", "content": "Mseleni in Northern Zululand is a remote geographically isolated improverished district in which about 2,000 persons suffer from a crippling joint disease similar to dysplasia epiphysealis multiplex and spondyloepiphyseal dysplasia. Eleven per cent of the men and 39% of the women in young adult life are seriously disabled and, with concurrent osteoarthritis, 90% of the population are grossly incapacitated in old age. Affected and unaffected control areas have been compared. Analysis of the climate, dietary factors, social taboos and soil and water does not implicate these as causes. Genetic studies, despite considerable field work, sociological surveys and laboratory investigations have not provided the answer. Despite a high incidence in the Mseleni area no disturbance of blood group percentages could be determined. The disease had dissimilarities from Kashin Beck disease in which fusarium fungus allegedly causes small joint polyarthritis. Fusarium sporotrichiella has not been grown in subtropical Zululand and thus could not be a causative agent. Mseleni disease also differs from Handigodu disease in Karnataka India, in which the hips are also destroyed in 80% of cases in the acute form of this disease which is brought on by environmental factors related to subtropical climate, low animal protein intake and intake of a variety of possibly toxic products of vegetable origin. Investigation of stored groundnuts in the affected districts has not revealed a fungus toxin in food which can as yet be implicated. A multifactorial genetic and environmental cause appears probable.", "contents": "Hip disease of Mseleni. Mseleni in Northern Zululand is a remote geographically isolated improverished district in which about 2,000 persons suffer from a crippling joint disease similar to dysplasia epiphysealis multiplex and spondyloepiphyseal dysplasia. Eleven per cent of the men and 39% of the women in young adult life are seriously disabled and, with concurrent osteoarthritis, 90% of the population are grossly incapacitated in old age. Affected and unaffected control areas have been compared. Analysis of the climate, dietary factors, social taboos and soil and water does not implicate these as causes. Genetic studies, despite considerable field work, sociological surveys and laboratory investigations have not provided the answer. Despite a high incidence in the Mseleni area no disturbance of blood group percentages could be determined. The disease had dissimilarities from Kashin Beck disease in which fusarium fungus allegedly causes small joint polyarthritis. Fusarium sporotrichiella has not been grown in subtropical Zululand and thus could not be a causative agent. Mseleni disease also differs from Handigodu disease in Karnataka India, in which the hips are also destroyed in 80% of cases in the acute form of this disease which is brought on by environmental factors related to subtropical climate, low animal protein intake and intake of a variety of possibly toxic products of vegetable origin. Investigation of stored groundnuts in the affected districts has not revealed a fungus toxin in food which can as yet be implicated. A multifactorial genetic and environmental cause appears probable."}
{"id": "PMID:477112", "title": "Penetration of gown material by organisms from the surgical team.", "content": "The routes by which viable organisms shed by the surgical team reach the wound are not yet fully understood. Bacteriologic studies show that shedding is greater in surgeons than their assistants or scrub nurses, and is increased by activity and temperature, but is primarily related to the individual's shedding characteristics. Comparison of the ability of 3 types of cotton gowns to contain the surgical team's bacterial effluent shows that the body exhaust system gowns developed by Charnley are superior to more conventional gowns. It is clear that viable organisms can penetrate very closely woven Ventile (pore size 10 microns) as well as balloon cloth (pore size 50 microns). Ventile used without a body exhaust system does not appear to increase gown efficiency. Penetration of gown material by organisms from the surgical team is responsible for 20% of wound contamination. The gown glove cuff junction is an important leakage point for organisms shed by the surgical team. A newly designed \"mitt\" cuff more effectively seals this area than the conventional stockinette cuff.", "contents": "Penetration of gown material by organisms from the surgical team. The routes by which viable organisms shed by the surgical team reach the wound are not yet fully understood. Bacteriologic studies show that shedding is greater in surgeons than their assistants or scrub nurses, and is increased by activity and temperature, but is primarily related to the individual's shedding characteristics. Comparison of the ability of 3 types of cotton gowns to contain the surgical team's bacterial effluent shows that the body exhaust system gowns developed by Charnley are superior to more conventional gowns. It is clear that viable organisms can penetrate very closely woven Ventile (pore size 10 microns) as well as balloon cloth (pore size 50 microns). Ventile used without a body exhaust system does not appear to increase gown efficiency. Penetration of gown material by organisms from the surgical team is responsible for 20% of wound contamination. The gown glove cuff junction is an important leakage point for organisms shed by the surgical team. A newly designed \"mitt\" cuff more effectively seals this area than the conventional stockinette cuff."}
{"id": "PMID:477113", "title": "Internal derangement of the sternoclavicular joint.", "content": "Indirect injuries to the sternoclavicular joint can result in an internal derangement. The major injury is to the intra-articular disk. The condition is generally caused by indirect injury to the shoulder, and causes tenderness, mild swelling and a clicking sensation over the joint. An arthrogram may well prove helpful in diagnosis. The lesion is treated by simple disk excision.", "contents": "Internal derangement of the sternoclavicular joint. Indirect injuries to the sternoclavicular joint can result in an internal derangement. The major injury is to the intra-articular disk. The condition is generally caused by indirect injury to the shoulder, and causes tenderness, mild swelling and a clicking sensation over the joint. An arthrogram may well prove helpful in diagnosis. The lesion is treated by simple disk excision."}
{"id": "PMID:477114", "title": "Distal symphalangism: symbrachydactylism arising in a family with distal symphalangism.", "content": "A recent survey of congenital hand anomalies has revealed that symphalangism of the distal interphalangeal joint is more frequent than had previously been reported. To date, however, only 3 familial pedigrees have been documented in the literature. The family reported in this article had 9 individuals spanning 4 generations affected with symphalangism of the distal interphalangeal joint. In all cases, this was associated with ankylosis of the distal interphalangeal joint of the second through fifth toes. One child in the family also had brachyphalangism bilaterally as well as a bifid distal phalanx of the thumb. The world's literature does not seem to include a similar case. As with previous cases of fusion of the distal interphalangeal joint, there was little functional disability resulting from this anomaly and we do not recommend operative procedures to restore motion.", "contents": "Distal symphalangism: symbrachydactylism arising in a family with distal symphalangism. A recent survey of congenital hand anomalies has revealed that symphalangism of the distal interphalangeal joint is more frequent than had previously been reported. To date, however, only 3 familial pedigrees have been documented in the literature. The family reported in this article had 9 individuals spanning 4 generations affected with symphalangism of the distal interphalangeal joint. In all cases, this was associated with ankylosis of the distal interphalangeal joint of the second through fifth toes. One child in the family also had brachyphalangism bilaterally as well as a bifid distal phalanx of the thumb. The world's literature does not seem to include a similar case. As with previous cases of fusion of the distal interphalangeal joint, there was little functional disability resulting from this anomaly and we do not recommend operative procedures to restore motion."}
{"id": "PMID:477115", "title": "Morton's neuroma--pathogenesis and ultrastructure.", "content": "In an attempt to clarify the pathogenesis of Morton's neuroma, 106 documented cases were analyzed by multiple staining techniques and electron microscopy. The lesion was found to consist of a progressive fibrosis which enveloped and disrupted nerves and arteries. No evidence of a nerve proliferation nor of a specific inflammatory process was encountered. Based on these observations, it is concluded that repeated trauma in the connective tissue elements, including nerves and arteries in the interdigital clefts, lead to a reactive overgrowth of connective tissue (scarring) that disrupts the nerves and the arteries. Sclerosis of the arteries and the narrowing of their lumen contributes to ischemia and further nerve atrophy. The nerves and arteries caught in this reactive scar become more sensitive to pressure and cause characteristic pain.", "contents": "Morton's neuroma--pathogenesis and ultrastructure. In an attempt to clarify the pathogenesis of Morton's neuroma, 106 documented cases were analyzed by multiple staining techniques and electron microscopy. The lesion was found to consist of a progressive fibrosis which enveloped and disrupted nerves and arteries. No evidence of a nerve proliferation nor of a specific inflammatory process was encountered. Based on these observations, it is concluded that repeated trauma in the connective tissue elements, including nerves and arteries in the interdigital clefts, lead to a reactive overgrowth of connective tissue (scarring) that disrupts the nerves and the arteries. Sclerosis of the arteries and the narrowing of their lumen contributes to ischemia and further nerve atrophy. The nerves and arteries caught in this reactive scar become more sensitive to pressure and cause characteristic pain."}
{"id": "PMID:477116", "title": "Growth of osteoid osteoma transplanted into athymic nude mice.", "content": "An osteoid osteoma, excised from the neck of the femur of a 23-year-old man, was cut into four 1.5 mm3 fragments and immediately transplanted into muscle pouches in athymic nude mice. One fragment was devitalized by lyophilization before implantation. The viable tumor cell xenografts grew, differentiated into uncalcified osteoid, and retained the characteristics of the original tumor. The killed implants were resorbed, but both the surviving viable and nonviable tumor tissue induced the connective tissue cells of the mouse host bed to proliferate and differentiate into normal cartilage and calcified bone. The mouse new bone deposits were remodeled and colonized by bone marrow, a tissue not seen in osteoid osteomas. These observations suggest that the sclerotic bone shell characteristic of osteoid osteomas may be an inductive reaction of host bed tissue to an osteoma cell product that is comparable to bone morphogenetic protein (BMP) produced by normal bone cells and transferred by normal bone matrix.", "contents": "Growth of osteoid osteoma transplanted into athymic nude mice. An osteoid osteoma, excised from the neck of the femur of a 23-year-old man, was cut into four 1.5 mm3 fragments and immediately transplanted into muscle pouches in athymic nude mice. One fragment was devitalized by lyophilization before implantation. The viable tumor cell xenografts grew, differentiated into uncalcified osteoid, and retained the characteristics of the original tumor. The killed implants were resorbed, but both the surviving viable and nonviable tumor tissue induced the connective tissue cells of the mouse host bed to proliferate and differentiate into normal cartilage and calcified bone. The mouse new bone deposits were remodeled and colonized by bone marrow, a tissue not seen in osteoid osteomas. These observations suggest that the sclerotic bone shell characteristic of osteoid osteomas may be an inductive reaction of host bed tissue to an osteoma cell product that is comparable to bone morphogenetic protein (BMP) produced by normal bone cells and transferred by normal bone matrix."}
{"id": "PMID:477117", "title": "Long-term follow-up of Charnley arthroplasty of the hip.", "content": "A review of 11.5 year (average interval) follow-up examination of 409 low friction arthroplasties performed included only 3 late failures. The excellent results of the successes have been fully maintained for as long as 14 years. This was true with regard to pain, function of walking and mobility. Resorption of the calcar femorale was rare and no correlation was found with the wear of the socket. The average wear of 1.5 mm was less than predicted by Professor Sir John Charnley. Although 10 patients (7.8%) had excessive wear no patient is at present near the stage of requiring replacement of the socket for this reason. No adverse reaction was found to the implant or the cement.", "contents": "Long-term follow-up of Charnley arthroplasty of the hip. A review of 11.5 year (average interval) follow-up examination of 409 low friction arthroplasties performed included only 3 late failures. The excellent results of the successes have been fully maintained for as long as 14 years. This was true with regard to pain, function of walking and mobility. Resorption of the calcar femorale was rare and no correlation was found with the wear of the socket. The average wear of 1.5 mm was less than predicted by Professor Sir John Charnley. Although 10 patients (7.8%) had excessive wear no patient is at present near the stage of requiring replacement of the socket for this reason. No adverse reaction was found to the implant or the cement."}
{"id": "PMID:477118", "title": "The ultrastructural localization of secretory acid phosphatase in giant cell tumor of bone.", "content": "Three cases of giant cell tumor of bone were studied with the light and electron microscopes to determine the histochemical and cytochemical distribution of acid phosphatase isoenzymes. Using beta-glycerophosphate as a nonspecific substrate, acid phosphatase was found in the giant cells as well as the stromal cells. Ultrastructurally, using this substrate, the enzyme was found to be associated with lysosomes in the stromal cells and giant cells and also with the profuse system of tubules and vesicles in the cytoplasm of the giant cells. Using phosphorylcholine and D-ephedrinephosphate, which are substrates for a specific secretory isoenzyme of acid phosphatase, activity was found only in the vesicles of the giant cell cytoplasm. Lysosomes did not show activity with these substrates. Multinucleated cells of giant cell tumor contain a specific secretory type of acid phosphatase which is not present in the stromal cells. This suggests that the giant cells are attempting to form a secretory system similar to osteoclasts and that the vesicles in the cytoplasm which contain this acid phosphates are the morphologic expression of an abortive secretory system.", "contents": "The ultrastructural localization of secretory acid phosphatase in giant cell tumor of bone. Three cases of giant cell tumor of bone were studied with the light and electron microscopes to determine the histochemical and cytochemical distribution of acid phosphatase isoenzymes. Using beta-glycerophosphate as a nonspecific substrate, acid phosphatase was found in the giant cells as well as the stromal cells. Ultrastructurally, using this substrate, the enzyme was found to be associated with lysosomes in the stromal cells and giant cells and also with the profuse system of tubules and vesicles in the cytoplasm of the giant cells. Using phosphorylcholine and D-ephedrinephosphate, which are substrates for a specific secretory isoenzyme of acid phosphatase, activity was found only in the vesicles of the giant cell cytoplasm. Lysosomes did not show activity with these substrates. Multinucleated cells of giant cell tumor contain a specific secretory type of acid phosphatase which is not present in the stromal cells. This suggests that the giant cells are attempting to form a secretory system similar to osteoclasts and that the vesicles in the cytoplasm which contain this acid phosphates are the morphologic expression of an abortive secretory system."}
{"id": "PMID:477119", "title": "The effect of calcitonin on fracture healing.", "content": "Corrective osteotomy of the long bones may be required in patients with Paget's disease. Experiments on rabbits suggest that the safety of the procedures is of concern while the patient receives Calcitonin. Twenty-four rabbits were divided into 4 groups of 6 animals. Half of each group received maintenance doses of Calcitonin subcutaneously. In all animals the left and right ulnae were osteotomized through middiaphysis. The right ulna was fixed with a \"mini\" dynamic compression plate. The left ulna was not immobilized. Each group of animals was sacrificed at successive intervals of 3 weeks. Histologically, no difference was found in the healing of the fracture of test or control animals. Radiographically, the fracture line persisted longer in animals receiving Calcitonin (6 weeks vs 3 weeks). All fractures healed by 9 weeks. The fracture line disappeared more rapidly in all the immobilized bones. Based on these observations, administration of Calcitonin is not a contraindication for corrective osteotomy in Paget's disease. Osteotomies should be rigidly fixed to maintain the correction achieved surgically until healing is established.", "contents": "The effect of calcitonin on fracture healing. Corrective osteotomy of the long bones may be required in patients with Paget's disease. Experiments on rabbits suggest that the safety of the procedures is of concern while the patient receives Calcitonin. Twenty-four rabbits were divided into 4 groups of 6 animals. Half of each group received maintenance doses of Calcitonin subcutaneously. In all animals the left and right ulnae were osteotomized through middiaphysis. The right ulna was fixed with a \"mini\" dynamic compression plate. The left ulna was not immobilized. Each group of animals was sacrificed at successive intervals of 3 weeks. Histologically, no difference was found in the healing of the fracture of test or control animals. Radiographically, the fracture line persisted longer in animals receiving Calcitonin (6 weeks vs 3 weeks). All fractures healed by 9 weeks. The fracture line disappeared more rapidly in all the immobilized bones. Based on these observations, administration of Calcitonin is not a contraindication for corrective osteotomy in Paget's disease. Osteotomies should be rigidly fixed to maintain the correction achieved surgically until healing is established."}
{"id": "PMID:477123", "title": "A method for determining success following total hip replacement surgery.", "content": "The success of a total hip replacement surgery, using the hip evaluation form, was investigated in 2 groups of surgical patients which differed in terms of relative success of surgery. For the men, the group which did not respond as well to surgery had less education and were more retired individuals; this group also had more pain, less ambulation, and greater functional handicaps prior to surgery. For the women, there were no significant differences between the groups with regard to the demographic variables and the hip evaluation measures prior to surgery. The Minnesota multiphasic personality inventory administered following surgery suggests that the women who did not respond as well to surgery had more physical symptoms, anxiety, depression, and difficulties in relating to people.", "contents": "A method for determining success following total hip replacement surgery. The success of a total hip replacement surgery, using the hip evaluation form, was investigated in 2 groups of surgical patients which differed in terms of relative success of surgery. For the men, the group which did not respond as well to surgery had less education and were more retired individuals; this group also had more pain, less ambulation, and greater functional handicaps prior to surgery. For the women, there were no significant differences between the groups with regard to the demographic variables and the hip evaluation measures prior to surgery. The Minnesota multiphasic personality inventory administered following surgery suggests that the women who did not respond as well to surgery had more physical symptoms, anxiety, depression, and difficulties in relating to people."}
{"id": "PMID:477124", "title": "Risk factor assessment in 101 total hip arthroplasties: a medical perspective.", "content": "Our experience with 101 consecutive T.H.A.'s in 91 patients was examined in an attempt to identify preoperative risk factors for postoperative medical complications, especially pulmonary embolism and thrombophlebitis. Six per cent of patients developed postoperative clinical thrombophlebitis, and 8% a pulmonary embolism. Advancing age and previous venous thrombosis served as predictors for pulmonary embolism and thrombophlebitis, respectively (p less than 0.01), but obesity, venous varicosities, diabetes mellitus, cigarette smoking, previous pulmonary embolism, and length of surgery did not, for either. Fifty per cent of the patients with preoperative abnormal kidney function developed some form of medical complication postoperatively, a significant increase in risk (p less than 0.05) over patients with normal kidney function. We were unable to identify an increase in postoperative atelectasis or pneumonia associated with smoking or obesity in these patients. No consistent decrease in post-operative medical morbidity could be assigned to preoperative medical consultations, suggesting that we have not yet identified all significant risk factors. A thorough preoperative preparation and improvement in intra- and postoperative techniques and management may account for differences found in this study from traditionally held risk factors.", "contents": "Risk factor assessment in 101 total hip arthroplasties: a medical perspective. Our experience with 101 consecutive T.H.A.'s in 91 patients was examined in an attempt to identify preoperative risk factors for postoperative medical complications, especially pulmonary embolism and thrombophlebitis. Six per cent of patients developed postoperative clinical thrombophlebitis, and 8% a pulmonary embolism. Advancing age and previous venous thrombosis served as predictors for pulmonary embolism and thrombophlebitis, respectively (p less than 0.01), but obesity, venous varicosities, diabetes mellitus, cigarette smoking, previous pulmonary embolism, and length of surgery did not, for either. Fifty per cent of the patients with preoperative abnormal kidney function developed some form of medical complication postoperatively, a significant increase in risk (p less than 0.05) over patients with normal kidney function. We were unable to identify an increase in postoperative atelectasis or pneumonia associated with smoking or obesity in these patients. No consistent decrease in post-operative medical morbidity could be assigned to preoperative medical consultations, suggesting that we have not yet identified all significant risk factors. A thorough preoperative preparation and improvement in intra- and postoperative techniques and management may account for differences found in this study from traditionally held risk factors."}
{"id": "PMID:477127", "title": "A method of management of the fractured stem in total hip replacement.", "content": "A method of removal of the fractured stem of a total hip replacement consists of drilling the distal fragment and extracting it through the open neck of the femur. Good exposure and careful preparation of the medullary canal to take a heavier stem in a well supported position will prevent stem fracture. Extended neck prosthesis may be indicated to ensure stability. Secure reattachment of the trochanter is essential.", "contents": "A method of management of the fractured stem in total hip replacement. A method of removal of the fractured stem of a total hip replacement consists of drilling the distal fragment and extracting it through the open neck of the femur. Good exposure and careful preparation of the medullary canal to take a heavier stem in a well supported position will prevent stem fracture. Extended neck prosthesis may be indicated to ensure stability. Secure reattachment of the trochanter is essential."}
{"id": "PMID:477126", "title": "Femoral component loosening after total hip arthroplasty.", "content": "To determine the long-term durability of total hip arthroplasty and to identify factors responsible for femoral component loosening, 106 total hip arthroplasties were reviewed in patients with a minimum postoperative period of 3 years. Seventy-four had Charnley-M\u00fcller prostheses and 32 had Charnley prostheses. Femoral component loosening occurred in 16 of 106 hips. Not all loose components were symptomatic. The femoral component at risk was the femoral Charnley-M\u00fcller type that had been inserted in varus position in a functioning active heavy patient who previously had had a femoral head prosthesis.", "contents": "Femoral component loosening after total hip arthroplasty. To determine the long-term durability of total hip arthroplasty and to identify factors responsible for femoral component loosening, 106 total hip arthroplasties were reviewed in patients with a minimum postoperative period of 3 years. Seventy-four had Charnley-M\u00fcller prostheses and 32 had Charnley prostheses. Femoral component loosening occurred in 16 of 106 hips. Not all loose components were symptomatic. The femoral component at risk was the femoral Charnley-M\u00fcller type that had been inserted in varus position in a functioning active heavy patient who previously had had a femoral head prosthesis."}
{"id": "PMID:477128", "title": "Methylmethacrylate metabolism in man. The hydrolysis of methylmethacrylate to methacrylic acid during total hip replacement.", "content": "Methylmethacrylate, the monomeric component of the polymethylmethacrylate cement used in orthopedic surgery, has been shown to undergo hydrolysis to methacrylic acid during hip replacement operations. Circulating levels of methacrylic acid were comparable with those of methylmethacrylate. Concentrations of both methylmethacrylate and methacrylic acid normally lay in the range 0--15 micrograms/cc. No correlation could be discerned between changes in the concentrations of methylmethacrylate and methacrylic acid, and changes in blood pressure.", "contents": "Methylmethacrylate metabolism in man. The hydrolysis of methylmethacrylate to methacrylic acid during total hip replacement. Methylmethacrylate, the monomeric component of the polymethylmethacrylate cement used in orthopedic surgery, has been shown to undergo hydrolysis to methacrylic acid during hip replacement operations. Circulating levels of methacrylic acid were comparable with those of methylmethacrylate. Concentrations of both methylmethacrylate and methacrylic acid normally lay in the range 0--15 micrograms/cc. No correlation could be discerned between changes in the concentrations of methylmethacrylate and methacrylic acid, and changes in blood pressure."}
{"id": "PMID:477129", "title": "Effect of gentamicin on shear and interface strengths of bone cement.", "content": "Antibiotic impregnated cement offers a potential method for salvage of infected total joint replacements. The addition of gentamicin in concentrations of .5, 1.0, and 2.0 per 40 significantly affects the shear strength of Palacos acrylic bone cement. The clinical significance of this strnegth drop is not clear. Large grained antibiotic additives have deleterious effect on shear strength. Measurements were made to determine the effect of gentamicin concentration on the bone-cement interface strength but no trend could be determined. The interface strength was much less than the strength of Palacos alone and suggested that bone strength is the limiting factor.", "contents": "Effect of gentamicin on shear and interface strengths of bone cement. Antibiotic impregnated cement offers a potential method for salvage of infected total joint replacements. The addition of gentamicin in concentrations of .5, 1.0, and 2.0 per 40 significantly affects the shear strength of Palacos acrylic bone cement. The clinical significance of this strnegth drop is not clear. Large grained antibiotic additives have deleterious effect on shear strength. Measurements were made to determine the effect of gentamicin concentration on the bone-cement interface strength but no trend could be determined. The interface strength was much less than the strength of Palacos alone and suggested that bone strength is the limiting factor."}
{"id": "PMID:477141", "title": "Differential diagnosis of jaundice by 99mTc-IDA hepatobiliary imaging.", "content": "The utility of 99mTc-IDA imaging in the differential diagnosis of jaundice was assessed. Although overall accuracy was 84%, this method is still inferior to the reported efficiency of ultrasonography and CT in making the correct diagnosis. Radionuclide imaging is a good alternative when these other modalities are not available and it functions well in an adjuvant capacity. The diagnostic limitations of 99mTc-dimethyl IDA imaging in cases of moderate to severe jaundice are largely overcome by the use of 99mTc-p-butyl IDA.", "contents": "Differential diagnosis of jaundice by 99mTc-IDA hepatobiliary imaging. The utility of 99mTc-IDA imaging in the differential diagnosis of jaundice was assessed. Although overall accuracy was 84%, this method is still inferior to the reported efficiency of ultrasonography and CT in making the correct diagnosis. Radionuclide imaging is a good alternative when these other modalities are not available and it functions well in an adjuvant capacity. The diagnostic limitations of 99mTc-dimethyl IDA imaging in cases of moderate to severe jaundice are largely overcome by the use of 99mTc-p-butyl IDA."}
{"id": "PMID:477142", "title": "Successful visualization of cortical malignant lesions on renal scintigram using 99mTc-dimercaptosuccinic acid.", "content": "The early (dynamic) and late (static) renal images obtained with 99mTc-dimercaptosuccinic acid (DMSA) were utilized to evaluate renal cortical malignant tumors in 30 cases of renal cell carcinoma each of which had been confirmed by operation or autopsy. Early images, performed 20--50 seconds after radionuclide administration, demonstrated abnormal vascular pools in the cortex. A cold area without DMSA uptake was demonstrated on the 2-hour or late images, reflecting a non-functioning renal mass. Lesions demonstrated with combined early and late images showed a good correlation with those demonstrated by renal angiography in 25 out of 27 cases. However, when almost all the renal tumor was replaced by necrotic lesions, the early image did not reveal hypervascularity. When a highly vascular renal tumor extended to the perinephric tissues, the tumor area was sometimes overestimated on the early image. It is almost impossible to diagnose renal cell carcinoma using radionuclide imaging alone. However, 99mTc-DMSA renal studies, using both early and late imaging, have proven to be a useful, noninvasive adjunct in the detection of malignant cortical lesions.", "contents": "Successful visualization of cortical malignant lesions on renal scintigram using 99mTc-dimercaptosuccinic acid. The early (dynamic) and late (static) renal images obtained with 99mTc-dimercaptosuccinic acid (DMSA) were utilized to evaluate renal cortical malignant tumors in 30 cases of renal cell carcinoma each of which had been confirmed by operation or autopsy. Early images, performed 20--50 seconds after radionuclide administration, demonstrated abnormal vascular pools in the cortex. A cold area without DMSA uptake was demonstrated on the 2-hour or late images, reflecting a non-functioning renal mass. Lesions demonstrated with combined early and late images showed a good correlation with those demonstrated by renal angiography in 25 out of 27 cases. However, when almost all the renal tumor was replaced by necrotic lesions, the early image did not reveal hypervascularity. When a highly vascular renal tumor extended to the perinephric tissues, the tumor area was sometimes overestimated on the early image. It is almost impossible to diagnose renal cell carcinoma using radionuclide imaging alone. However, 99mTc-DMSA renal studies, using both early and late imaging, have proven to be a useful, noninvasive adjunct in the detection of malignant cortical lesions."}
{"id": "PMID:477143", "title": "The effect of diaphragmatic attenuation on 201Tl images.", "content": "The effect of diaphragmatic attenuation on 231Ti images of the heart was studied by intermittent recording with patients in inspiration and expiration. These studies suggest that the commonly observed posteroinferior defects in the left lateral projection can be explained by this mechanism. Possible effects on the anterior view are also suggested.", "contents": "The effect of diaphragmatic attenuation on 201Tl images. The effect of diaphragmatic attenuation on 231Ti images of the heart was studied by intermittent recording with patients in inspiration and expiration. These studies suggest that the commonly observed posteroinferior defects in the left lateral projection can be explained by this mechanism. Possible effects on the anterior view are also suggested."}
{"id": "PMID:477144", "title": "Adrenal gland imaging with multiplane tomography.", "content": "Multiplane tomography can be employed successfully to image the adrenal glands after the intravenous administration of 6 beta-131I-iodomethyl-19-nor cholest-5(10)-en-3 beta-ol (NP-59). The tomographic display allows separation of the adrenal glands from other organs that often obscure the adrenal image when gamma cameras are used and the depth of the adrenal glands may be determined without obtaining lateral views.", "contents": "Adrenal gland imaging with multiplane tomography. Multiplane tomography can be employed successfully to image the adrenal glands after the intravenous administration of 6 beta-131I-iodomethyl-19-nor cholest-5(10)-en-3 beta-ol (NP-59). The tomographic display allows separation of the adrenal glands from other organs that often obscure the adrenal image when gamma cameras are used and the depth of the adrenal glands may be determined without obtaining lateral views."}
{"id": "PMID:477145", "title": "Cisternography and CT scanning with 111In-DTPA in evaluation of posterior fossa arachnoid cysts.", "content": "A patient with a large, long-standing posterior fossa arachnoid cyst was evaluated. Computed tomography was used to demonstrate the cyst. 1111In-DTPA cisternography demonstrated the communication of the cyst with the subarachnoid space, stasis of the 111In in the cystic space, and incomplete ascent of cerebrospinal fluid over the cerebral hemispheres.", "contents": "Cisternography and CT scanning with 111In-DTPA in evaluation of posterior fossa arachnoid cysts. A patient with a large, long-standing posterior fossa arachnoid cyst was evaluated. Computed tomography was used to demonstrate the cyst. 1111In-DTPA cisternography demonstrated the communication of the cyst with the subarachnoid space, stasis of the 111In in the cystic space, and incomplete ascent of cerebrospinal fluid over the cerebral hemispheres."}
{"id": "PMID:477146", "title": "Localization of bone-seeking agent within a desmoid tumor.", "content": "This is a case report of a patient who had a desmoid tumor which localized 99mTc-diphosphonate. The mechanism for this localization was felt to be the affinity of the bone agent for the immature proliferating collagen in this tumor.", "contents": "Localization of bone-seeking agent within a desmoid tumor. This is a case report of a patient who had a desmoid tumor which localized 99mTc-diphosphonate. The mechanism for this localization was felt to be the affinity of the bone agent for the immature proliferating collagen in this tumor."}
{"id": "PMID:477149", "title": "Accumulation of 99mTc-methylene diphosphonate in an adenocarcinoma of the lung.", "content": "Increased accumulation of 99mTc-methylene diphosphonate (MDP) was observed in an adenocarcinoma of the lung during the performance of routine bone imaging.", "contents": "Accumulation of 99mTc-methylene diphosphonate in an adenocarcinoma of the lung. Increased accumulation of 99mTc-methylene diphosphonate (MDP) was observed in an adenocarcinoma of the lung during the performance of routine bone imaging."}
{"id": "PMID:477151", "title": "Reliability of stress thallium-201 scanning in the clinical evaluation of coronary artery disease.", "content": "Stress thallium-201 scanning proved to be a highly sensitive and specific screening procedure for myocardial ischemia in 52 consecutive cases with coronary arteriograms. Stress thallium-201 scanning was considerably more accurate and more specific than the exercise electrocardiogram (ECG) as evidenced by a sensitivity of 83% compared to 55%, and a specificity of 94% compared to 82%. When submaximal stress tests were excluded, improved sensitivity of thallium scan to exercise ECG was 95% to 64% with no change in specificity. Factors producing negative thallium scans in the presence of coronary artery disease were: delay in obtaining the first post-exercise scan, positioning and observing variability, severe three-vessel disease uniformly affecting all myocardial segments, and failure to achieve maximal stress testing due to attenuation of the heart-rate response by propranolol or somatic complaints.", "contents": "Reliability of stress thallium-201 scanning in the clinical evaluation of coronary artery disease. Stress thallium-201 scanning proved to be a highly sensitive and specific screening procedure for myocardial ischemia in 52 consecutive cases with coronary arteriograms. Stress thallium-201 scanning was considerably more accurate and more specific than the exercise electrocardiogram (ECG) as evidenced by a sensitivity of 83% compared to 55%, and a specificity of 94% compared to 82%. When submaximal stress tests were excluded, improved sensitivity of thallium scan to exercise ECG was 95% to 64% with no change in specificity. Factors producing negative thallium scans in the presence of coronary artery disease were: delay in obtaining the first post-exercise scan, positioning and observing variability, severe three-vessel disease uniformly affecting all myocardial segments, and failure to achieve maximal stress testing due to attenuation of the heart-rate response by propranolol or somatic complaints."}
{"id": "PMID:477152", "title": "Accumulation of 201Tl-chloride in a parathyroid adenoma.", "content": "A 201Tl-chloride sacn was performed on a patient with primary hyperparathyroidism. 201Tl-chloride accumulated in the region of the right lower thyroidal pole and a diagnosis of parathyroid adenoma was confirmed intraoperatively. 201Tl-chloride imaging of a hyperfunctioning parathyroid gland seems to be useful for the detection and preoperative localization of a parathyroid adenoma.", "contents": "Accumulation of 201Tl-chloride in a parathyroid adenoma. A 201Tl-chloride sacn was performed on a patient with primary hyperparathyroidism. 201Tl-chloride accumulated in the region of the right lower thyroidal pole and a diagnosis of parathyroid adenoma was confirmed intraoperatively. 201Tl-chloride imaging of a hyperfunctioning parathyroid gland seems to be useful for the detection and preoperative localization of a parathyroid adenoma."}
{"id": "PMID:477154", "title": "The Anger camera and the pertechnetate ion in the routine evaluation of thyroid uptake and imaging.", "content": "The thyroid traps the pertechnetate ion and iodine in a similar manner. Thus, 99mTc-pertechnetate may be used for thyroid uptake studies (including stimulation and suppression tests)8 as well as imaging. Images are obtained after an intravenous administration of 2--4 mCi of 99mTc-pertechnetate, and the scan is preceded by a trapping index at 20 minutes, measured as the neck to thigh ratio. In 1973, we compared 99mTc-pertechnetate with 131I in diagnostic uptake and imaging studies of the thyroid gland in 100 patients. We have had experience with an additional 100 patients, and modified our procedure so that the Anger camera with pinhole collimator will adequately replace the standard scintillation probe. Using the camera alone, with the 99mTc-pertechnetate makes the study less expensive and briefer, with no loss of information. Routine use of the scan aids in distinguishing diffuse from localized diseases.", "contents": "The Anger camera and the pertechnetate ion in the routine evaluation of thyroid uptake and imaging. The thyroid traps the pertechnetate ion and iodine in a similar manner. Thus, 99mTc-pertechnetate may be used for thyroid uptake studies (including stimulation and suppression tests)8 as well as imaging. Images are obtained after an intravenous administration of 2--4 mCi of 99mTc-pertechnetate, and the scan is preceded by a trapping index at 20 minutes, measured as the neck to thigh ratio. In 1973, we compared 99mTc-pertechnetate with 131I in diagnostic uptake and imaging studies of the thyroid gland in 100 patients. We have had experience with an additional 100 patients, and modified our procedure so that the Anger camera with pinhole collimator will adequately replace the standard scintillation probe. Using the camera alone, with the 99mTc-pertechnetate makes the study less expensive and briefer, with no loss of information. Routine use of the scan aids in distinguishing diffuse from localized diseases."}
{"id": "PMID:477155", "title": "Primary tuberculosis diagnosed by gallium scan.", "content": "We report a case of tuberculosis which, although clinically unsuspected, was diagnosed on a 67Ga scan. It is well known that 67Ga scintigraphy may be used as an adjunct to radiography for assessing extent and localization of inflammatory activity and for follow-up and progression evaluation of diffuse granulomatous diseases such as sarcoid and tuberculosis.", "contents": "Primary tuberculosis diagnosed by gallium scan. We report a case of tuberculosis which, although clinically unsuspected, was diagnosed on a 67Ga scan. It is well known that 67Ga scintigraphy may be used as an adjunct to radiography for assessing extent and localization of inflammatory activity and for follow-up and progression evaluation of diffuse granulomatous diseases such as sarcoid and tuberculosis."}
{"id": "PMID:477156", "title": "Cranial cholesteatoma: unusual 99mTc-Sn polyphosphate and 99mTc-pertechnetate scintiphotos.", "content": "Results of the brain and bone scintigraphy in a case of cranial cholesteatoma are described and the differential diagnosis is discussed. With both techniques, the lesion is characterized by diminished central and increased peripheral activity.", "contents": "Cranial cholesteatoma: unusual 99mTc-Sn polyphosphate and 99mTc-pertechnetate scintiphotos. Results of the brain and bone scintigraphy in a case of cranial cholesteatoma are described and the differential diagnosis is discussed. With both techniques, the lesion is characterized by diminished central and increased peripheral activity."}
{"id": "PMID:477163", "title": "Gallium scan in recurrent Hodgkin's disease in children.", "content": "In 18 of 88 children with biopsy proven and previously untreated Hodgkin's disease, recurrence developed during a period from four to 53 months after therapy (median period, 22 months). In 16 patients in whom gallium scans were performed, 21 positive gallium scans were obtained during 26 episodes of recurrence. Abnormalities were noted in half of them during a period from one to 10 months prior to physical, laboratory, radiographic or histologic confirmation of recurrence (median period about 5 months). We have concluded that the gallium scan is very useful in initial workup and is sensitive in detecting early recurrence in children with Hodgkin's disease. Such scans are indicated when there is clinical suspicion of recurrence, when other modalities are unavailable or when the results of other studies are equivocal.", "contents": "Gallium scan in recurrent Hodgkin's disease in children. In 18 of 88 children with biopsy proven and previously untreated Hodgkin's disease, recurrence developed during a period from four to 53 months after therapy (median period, 22 months). In 16 patients in whom gallium scans were performed, 21 positive gallium scans were obtained during 26 episodes of recurrence. Abnormalities were noted in half of them during a period from one to 10 months prior to physical, laboratory, radiographic or histologic confirmation of recurrence (median period about 5 months). We have concluded that the gallium scan is very useful in initial workup and is sensitive in detecting early recurrence in children with Hodgkin's disease. Such scans are indicated when there is clinical suspicion of recurrence, when other modalities are unavailable or when the results of other studies are equivocal."}
{"id": "PMID:477164", "title": "Abnormal images of right heart disorders.", "content": "Increasing attention is being given to radioisotopic studies of the right heart. Measurement of right ventricular ejection fraction has become more common. We present examples of cardiac images which were obtained during the scintigraphic assessment of right sided cardiac disorders. Images of the first pass, of the gated cardiac study, and of the nongated cardiac blood pools offer complementary information to the overall radionuclide evaluation of right heart disease.", "contents": "Abnormal images of right heart disorders. Increasing attention is being given to radioisotopic studies of the right heart. Measurement of right ventricular ejection fraction has become more common. We present examples of cardiac images which were obtained during the scintigraphic assessment of right sided cardiac disorders. Images of the first pass, of the gated cardiac study, and of the nongated cardiac blood pools offer complementary information to the overall radionuclide evaluation of right heart disease."}
{"id": "PMID:477166", "title": "Brain scintigraphy in arteriovenous malformation.", "content": "Brain scintigraphy demonstrated an unusual image pattern which was associated with an arteriovenous malformation (AVM). A preoperative diagnosis of AVM was suspected on the radionuclide study and confirmed by contrast angiography and surgery. We review the criteria established for diagnosis of AVM by dynamic and static scintigraphy and discuss additional findings which can be helpful when noncharacteristic AVM presentations are encountered.", "contents": "Brain scintigraphy in arteriovenous malformation. Brain scintigraphy demonstrated an unusual image pattern which was associated with an arteriovenous malformation (AVM). A preoperative diagnosis of AVM was suspected on the radionuclide study and confirmed by contrast angiography and surgery. We review the criteria established for diagnosis of AVM by dynamic and static scintigraphy and discuss additional findings which can be helpful when noncharacteristic AVM presentations are encountered."}
{"id": "PMID:477165", "title": "Hepatic adenoma: imaging with different radionuclides.", "content": "A case of a benign hepatic-cell adenoma studies with dynamic radionuclide angiography and static imaging with different tracers is presented.", "contents": "Hepatic adenoma: imaging with different radionuclides. A case of a benign hepatic-cell adenoma studies with dynamic radionuclide angiography and static imaging with different tracers is presented."}
{"id": "PMID:477168", "title": "Regional cerebral blood flow measurement using a scintillation camera.", "content": "A scintillation camera connected to auxillary equipment with off-line data processing or connected to an on-line dedicated computer system permits measurement of hemispheric and regional cerebral blood flow. Reliable flow values are obtained from regions limited in size by spatial resolution and the count rates achieved. Flow measurements obtained with the camera are able to resolve inhomogeneities of cerebral circulation in normal subjects. In a variety of clinical conditions, the localization, severity and extent of flow alterations are shown. Results of flow measurements in individual cases elucidate the pathogenesis of neurologic deficits, quantify the damage to the brain, indicate therapeutic measures of potential value and permit an estimation of the further clinical course. With restricted spatial resolution, flow measurements after intravenous 133Xe injection are also feasible.", "contents": "Regional cerebral blood flow measurement using a scintillation camera. A scintillation camera connected to auxillary equipment with off-line data processing or connected to an on-line dedicated computer system permits measurement of hemispheric and regional cerebral blood flow. Reliable flow values are obtained from regions limited in size by spatial resolution and the count rates achieved. Flow measurements obtained with the camera are able to resolve inhomogeneities of cerebral circulation in normal subjects. In a variety of clinical conditions, the localization, severity and extent of flow alterations are shown. Results of flow measurements in individual cases elucidate the pathogenesis of neurologic deficits, quantify the damage to the brain, indicate therapeutic measures of potential value and permit an estimation of the further clinical course. With restricted spatial resolution, flow measurements after intravenous 133Xe injection are also feasible."}
{"id": "PMID:477173", "title": "Extrahepatic portal hypertension due to congenital obstruction of the portal vein and associated gross hepatic lobulation.", "content": "A 10-233k old girl presented with splenomegaly and recurrent hematemesis from esophageal varices. Splenoportography revealed a dilated extrahepatic portion of the portal vein with nonvisualization of its intrahepatic tributaries. The child died following an episode of hematemesis and was found to have a dilated portal vein which ended blindly. In addition, there was abnormal lobulation of the inferior surface of the liver which was not cirrhotic. The portal vascular anomaly, which presumably was responsible for the portal hypertension, was probably due to failure of communication between the embryonic vitelline veins or to atresia of the portal vein secondary to pressure from the abnormal hepatic lobulation in utero. It would appear that congenital factors may be significant in the etiology and pathogenesis of some cases of extrahepatic portal hypertension in early life and recognition of such developmental anomalies is of importantance in management.", "contents": "Extrahepatic portal hypertension due to congenital obstruction of the portal vein and associated gross hepatic lobulation. A 10-233k old girl presented with splenomegaly and recurrent hematemesis from esophageal varices. Splenoportography revealed a dilated extrahepatic portion of the portal vein with nonvisualization of its intrahepatic tributaries. The child died following an episode of hematemesis and was found to have a dilated portal vein which ended blindly. In addition, there was abnormal lobulation of the inferior surface of the liver which was not cirrhotic. The portal vascular anomaly, which presumably was responsible for the portal hypertension, was probably due to failure of communication between the embryonic vitelline veins or to atresia of the portal vein secondary to pressure from the abnormal hepatic lobulation in utero. It would appear that congenital factors may be significant in the etiology and pathogenesis of some cases of extrahepatic portal hypertension in early life and recognition of such developmental anomalies is of importantance in management."}
{"id": "PMID:477172", "title": "Iron poisoning: assessment of radiography in diagnosis and management.", "content": "Severe acute iron poisoning developed in a 1 1/2-year-old child who had eaten an iron preparation that resembles a popular chocolate candy. Tablets containing iron, with and without vitamins, and the sugar-coated candy of similar appearance, were radiographed with human gastric juice in vitro. Times of dissolution of the radiopaque tablets were noted, to assess the value of abdominal radiographs in the diagnosis and management of acute iron poisoning.", "contents": "Iron poisoning: assessment of radiography in diagnosis and management. Severe acute iron poisoning developed in a 1 1/2-year-old child who had eaten an iron preparation that resembles a popular chocolate candy. Tablets containing iron, with and without vitamins, and the sugar-coated candy of similar appearance, were radiographed with human gastric juice in vitro. Times of dissolution of the radiopaque tablets were noted, to assess the value of abdominal radiographs in the diagnosis and management of acute iron poisoning."}
{"id": "PMID:477174", "title": "Neonatal appendicitis.", "content": "Another example of neonatal appendicitis is presented, and the clinical details of 33 cases previously documented in the literature are reviewed. This rare condition is characterized by an inordinate delay in diagnosis, a high incidence of perforation of the appendix and a high mortality.", "contents": "Neonatal appendicitis. Another example of neonatal appendicitis is presented, and the clinical details of 33 cases previously documented in the literature are reviewed. This rare condition is characterized by an inordinate delay in diagnosis, a high incidence of perforation of the appendix and a high mortality."}
{"id": "PMID:477175", "title": "Collaborative research between a Department of Pediatrics and its clinical faculty.", "content": "Collaborative research involving members of the full-time and clinical faculties of a Department of Pediatrics increases our knowledge of problems encountered in primary care practice by bringing the practitioner's perspective on the nature of such problems and the population of patients he serves to the investigative arena. For the practitioner, active involvement in collaborative research is rewarding because he or she formulates the questions to be addressed and takes part in planning and implementing the research and reporting results. Through such collaboration, the academician gains immeasurably by being able to study common clinical problems in primary care settings, where they are encountered most often. To be successful, collaborative research must be a joint effort with ongoing communication and sharing of responsibilities between academicians and practitioners.", "contents": "Collaborative research between a Department of Pediatrics and its clinical faculty. Collaborative research involving members of the full-time and clinical faculties of a Department of Pediatrics increases our knowledge of problems encountered in primary care practice by bringing the practitioner's perspective on the nature of such problems and the population of patients he serves to the investigative arena. For the practitioner, active involvement in collaborative research is rewarding because he or she formulates the questions to be addressed and takes part in planning and implementing the research and reporting results. Through such collaboration, the academician gains immeasurably by being able to study common clinical problems in primary care settings, where they are encountered most often. To be successful, collaborative research must be a joint effort with ongoing communication and sharing of responsibilities between academicians and practitioners."}
{"id": "PMID:477176", "title": "Cholestyramine treatment of chronic diarrhea associated with immune deficiency syndrome.", "content": "A 5-year-old boy with known severe combined immunodeficiency disease presented with chronic diarrhea, malabsorption and retarded growth. Candida albicans was found in distal duodenal fluid, and invading the intestinal mucosa. Chronic diarrhea persisted after antimycotic therapy, but responded to treatment with cholestyramine. Repeated courses of cholestyramine resin over a 6-month period were required for complete resolution of the gastrointestinal symptomatology.", "contents": "Cholestyramine treatment of chronic diarrhea associated with immune deficiency syndrome. A 5-year-old boy with known severe combined immunodeficiency disease presented with chronic diarrhea, malabsorption and retarded growth. Candida albicans was found in distal duodenal fluid, and invading the intestinal mucosa. Chronic diarrhea persisted after antimycotic therapy, but responded to treatment with cholestyramine. Repeated courses of cholestyramine resin over a 6-month period were required for complete resolution of the gastrointestinal symptomatology."}
{"id": "PMID:477181", "title": "Kinetics of mucosal influx of glycylsarcosine, glycine and leucine into hamster jejunum and ileum in vitro.", "content": "1. This paper describes an investigation of the kinetics of influx of the dipeptide glycylsarcosine and the amino acids glycine and L-leucine into rings of everted hamster small intestine in vitro, in proximal and distal small intestine (jejunum and ileum). Results were expressed per unit wet weight of intestine. 2. At all concentrations studied (0.1--100 mmol/l), influx of glycylsarcosine was more rapid in the jejunum than in the ileum. In contrast, at all concentrations studied, influx of glycine and leucine was more rapid in the ileum than the jejunum. 3. Estimates of the simple diffusion component in total influx were made. This component became increasingly large as the substrate concentration was raised. After correction for simple diffusion, transport of all three substrates conformed to Michaelis-Menten kinetics in both jejunum and ileum. Values for simple diffusion, apparent Kt and Vmax, are reported. 4. Possibly physiological implications of the results are discussed, and it is pointed out that under experimental conditions similar to our own, simple diffusion is too large a component in total influx to be ignored.", "contents": "Kinetics of mucosal influx of glycylsarcosine, glycine and leucine into hamster jejunum and ileum in vitro. 1. This paper describes an investigation of the kinetics of influx of the dipeptide glycylsarcosine and the amino acids glycine and L-leucine into rings of everted hamster small intestine in vitro, in proximal and distal small intestine (jejunum and ileum). Results were expressed per unit wet weight of intestine. 2. At all concentrations studied (0.1--100 mmol/l), influx of glycylsarcosine was more rapid in the jejunum than in the ileum. In contrast, at all concentrations studied, influx of glycine and leucine was more rapid in the ileum than the jejunum. 3. Estimates of the simple diffusion component in total influx were made. This component became increasingly large as the substrate concentration was raised. After correction for simple diffusion, transport of all three substrates conformed to Michaelis-Menten kinetics in both jejunum and ileum. Values for simple diffusion, apparent Kt and Vmax, are reported. 4. Possibly physiological implications of the results are discussed, and it is pointed out that under experimental conditions similar to our own, simple diffusion is too large a component in total influx to be ignored."}
{"id": "PMID:477182", "title": "Arterial wall renin and renal venous renin in the hypertensive rat.", "content": "1. Infusion of sufficient renin to raise the blood pressure of normal rats to hypertensive levels resulted in increased renin in the arterial wall. 2. Arterial wall renin and renal venous renin were normal in younger spontaneously hypertensive rats, but in older spontaneously hypertensive rats arterial wall renin was significantly increased and renal venous renin was significantly decreased. 3. Arterial wall renin in rats with either acute or chronic two-kidney Goldblatt renal hypertension was significantly increased, whereas circulatory renin was elevated in the former, but depressed in the latter. 4. Arterial wall renin may play a role in the maintenance of acute and chronic renal hypertension and also perhaps of spontaneous hypertension of long duration in older rats.", "contents": "Arterial wall renin and renal venous renin in the hypertensive rat. 1. Infusion of sufficient renin to raise the blood pressure of normal rats to hypertensive levels resulted in increased renin in the arterial wall. 2. Arterial wall renin and renal venous renin were normal in younger spontaneously hypertensive rats, but in older spontaneously hypertensive rats arterial wall renin was significantly increased and renal venous renin was significantly decreased. 3. Arterial wall renin in rats with either acute or chronic two-kidney Goldblatt renal hypertension was significantly increased, whereas circulatory renin was elevated in the former, but depressed in the latter. 4. Arterial wall renin may play a role in the maintenance of acute and chronic renal hypertension and also perhaps of spontaneous hypertension of long duration in older rats."}
{"id": "PMID:477183", "title": "Fluorescent lipid-peroxidation products in synovial fluid.", "content": "1. Samples and extracts from synovial effusions were examined for the presence of fluorescent degradation products of free-radical oxidation (peroxidation). 2. Two classes of fluorescent compounds were identified and their fluorescence and physical properties are described. The fluorescence in the aqueous methanol phase of the extracts had the characteristics of conjugated Schiff bases. 3. Changes in synovial fluid and synovial fluid extracts in vitro induced by u.v. irradiation, prolonged incubation and the enzymic generation of oxidizing free radicals, with or without admixed peroxidizing arachidonic acid, and in the absence and presence of antioxidants, suggest that the fluorescent material is derived from peroxidized polyunsaturated lipids in damaged synovial tissue.", "contents": "Fluorescent lipid-peroxidation products in synovial fluid. 1. Samples and extracts from synovial effusions were examined for the presence of fluorescent degradation products of free-radical oxidation (peroxidation). 2. Two classes of fluorescent compounds were identified and their fluorescence and physical properties are described. The fluorescence in the aqueous methanol phase of the extracts had the characteristics of conjugated Schiff bases. 3. Changes in synovial fluid and synovial fluid extracts in vitro induced by u.v. irradiation, prolonged incubation and the enzymic generation of oxidizing free radicals, with or without admixed peroxidizing arachidonic acid, and in the absence and presence of antioxidants, suggest that the fluorescent material is derived from peroxidized polyunsaturated lipids in damaged synovial tissue."}
{"id": "PMID:477185", "title": "Time-dependent changes in prostaglandin excretion in response to frusemide in man.", "content": "1. To evaluate in man by a non-invasive technique the possible role of prostaglandin (PG) compounds in initial renal haemodynamic effects after frusemide we studied the urinary excretion of PGE2 and of PGF2 alpha before and at 15 min and 120 min after intravenous injection of this drug. 2. An increase of PGE2 and of PGF2 alpha excretion was found in all 19 volunteer subjects within 15 min after frusemide, and PG excretion had returned towards control values at 120 min. The stimulation of PGF2 alpha excretion by frusemide was markedly lower in men than in women, but this difference was statistically not significant. 3. No clear-cut relation was found between urinary PG compounds, on the one hand, and urinary volume, urinary sodium and urinary potassium, on the other hand, during the study. 4. The results support the assumption that the rapid increase of urinary PG compounds after frusemide, which parallels the changes in renal haemodynamics, may be an indicator of an activation of the PG system, in part or predominantly, in the vascular compartment.", "contents": "Time-dependent changes in prostaglandin excretion in response to frusemide in man. 1. To evaluate in man by a non-invasive technique the possible role of prostaglandin (PG) compounds in initial renal haemodynamic effects after frusemide we studied the urinary excretion of PGE2 and of PGF2 alpha before and at 15 min and 120 min after intravenous injection of this drug. 2. An increase of PGE2 and of PGF2 alpha excretion was found in all 19 volunteer subjects within 15 min after frusemide, and PG excretion had returned towards control values at 120 min. The stimulation of PGF2 alpha excretion by frusemide was markedly lower in men than in women, but this difference was statistically not significant. 3. No clear-cut relation was found between urinary PG compounds, on the one hand, and urinary volume, urinary sodium and urinary potassium, on the other hand, during the study. 4. The results support the assumption that the rapid increase of urinary PG compounds after frusemide, which parallels the changes in renal haemodynamics, may be an indicator of an activation of the PG system, in part or predominantly, in the vascular compartment."}
{"id": "PMID:477186", "title": "Binding of human serum ferritin to concanavalin A.", "content": "1. A high proportion of the ferritin in normal serum binds to concanavalin A. Binding is prevented by the addition of alpha-D-methylglucoside to the reaction mixture. 2. Ferritin in extracts of normal heart, liver and spleen or serum ferritin from patients with massive hepatic necrosis does not bind to concanavalin A. 3. Isoelectric focusing of preparations of serum ferritin from patients with primary haemochromatosis shows that the ferritin fraction binding to concanavalin A consists, predominantly, of the more acidic isoferritins. 4. These findings suggest that carbohydrate residues may be added to ferritin during its secretion into the plasma. Glycosylation may account for the heterogeneity of serum ferritin on isoelectric focusing. 5. Direct release of intracellular ferritin from damaged tissue may be indicated by an increase in the proportion of circulating ferritin which does not bind to concanavalin A. Such an increase has been found in sera from patients with iron overload.", "contents": "Binding of human serum ferritin to concanavalin A. 1. A high proportion of the ferritin in normal serum binds to concanavalin A. Binding is prevented by the addition of alpha-D-methylglucoside to the reaction mixture. 2. Ferritin in extracts of normal heart, liver and spleen or serum ferritin from patients with massive hepatic necrosis does not bind to concanavalin A. 3. Isoelectric focusing of preparations of serum ferritin from patients with primary haemochromatosis shows that the ferritin fraction binding to concanavalin A consists, predominantly, of the more acidic isoferritins. 4. These findings suggest that carbohydrate residues may be added to ferritin during its secretion into the plasma. Glycosylation may account for the heterogeneity of serum ferritin on isoelectric focusing. 5. Direct release of intracellular ferritin from damaged tissue may be indicated by an increase in the proportion of circulating ferritin which does not bind to concanavalin A. Such an increase has been found in sera from patients with iron overload."}
{"id": "PMID:477187", "title": "Niacin depletion in Parkinsonian patients treated with L-dopa, benserazide and carbidopa.", "content": "1. Benserazide and carbidopa, decarboxylase inhibitors used in the treatment of Parkinson's disease, have been shown to inhibit the enzyme kynurenine hydrolase in rat and mouse liver. This results in reduced synthesis of nicotinamide coenzymes from tryptophan, and hence an increased reliance on dietary niacin. 2. Pellagra might be expected as a result of this inhibition of endogenous synthesis of nicotinamide nucleotides, but has not been reported in patients treated with either drug. 3. The urinary excretion of N1-methyl-nicotinamide, a product of nicotinamide nucleotide metabolism, is considerably reduced in patients treated with dopa alone or in combination with an inhibitor of peripheral dopa decarboxylase, to as low as 40% of the control value. This means that many of these patients could be classified as 'at risk' of niacin deficiency, even if not frankly deficient. 4. Patients treated with dopa plus a decarboxylase inhibitor, but not those treated with dopa alone, also show a reduced excretion of xanthurenic acid, and an increased excretion of kynurenine, as would be expected after inhibition of the kynurenine pathway, and possibly indicative of marginal vitamin B6 deficiency.", "contents": "Niacin depletion in Parkinsonian patients treated with L-dopa, benserazide and carbidopa. 1. Benserazide and carbidopa, decarboxylase inhibitors used in the treatment of Parkinson's disease, have been shown to inhibit the enzyme kynurenine hydrolase in rat and mouse liver. This results in reduced synthesis of nicotinamide coenzymes from tryptophan, and hence an increased reliance on dietary niacin. 2. Pellagra might be expected as a result of this inhibition of endogenous synthesis of nicotinamide nucleotides, but has not been reported in patients treated with either drug. 3. The urinary excretion of N1-methyl-nicotinamide, a product of nicotinamide nucleotide metabolism, is considerably reduced in patients treated with dopa alone or in combination with an inhibitor of peripheral dopa decarboxylase, to as low as 40% of the control value. This means that many of these patients could be classified as 'at risk' of niacin deficiency, even if not frankly deficient. 4. Patients treated with dopa plus a decarboxylase inhibitor, but not those treated with dopa alone, also show a reduced excretion of xanthurenic acid, and an increased excretion of kynurenine, as would be expected after inhibition of the kynurenine pathway, and possibly indicative of marginal vitamin B6 deficiency."}
{"id": "PMID:477188", "title": "The influence of ammonia and octanoic acid on liver regeneration in the rat.", "content": "1. We have investigated the effect of intermittent doses of ammonia and octanoic acid on liver regeneration in the rat after partial hepatectomy. Regeneration was assessed by uptake of tritiated thymidine into liver deoxyribonucleic acid. 2. We have found a reduction in hepatic regenerative response in animals treated with ammonium acetate as a source of ammonia. 3. No inhibiting effect of octanoic acid on liver regeneration was found, at the dose used. 4. The implications of the results for management of fulminant hepatic failure are discussed.", "contents": "The influence of ammonia and octanoic acid on liver regeneration in the rat. 1. We have investigated the effect of intermittent doses of ammonia and octanoic acid on liver regeneration in the rat after partial hepatectomy. Regeneration was assessed by uptake of tritiated thymidine into liver deoxyribonucleic acid. 2. We have found a reduction in hepatic regenerative response in animals treated with ammonium acetate as a source of ammonia. 3. No inhibiting effect of octanoic acid on liver regeneration was found, at the dose used. 4. The implications of the results for management of fulminant hepatic failure are discussed."}
{"id": "PMID:477189", "title": "Presence of apolipoprotein-CII in commercially available albumin fractions.", "content": "1. Commercially available bovine serum albumin as Cohn fraction V was demonstrated to contain small amounts of apolipoprotein-CII. 2. This apolipoprotein activated lipoprotein lipase in the same way as apolipoprotein-CII purified from human very-low-density lipoproteins.", "contents": "Presence of apolipoprotein-CII in commercially available albumin fractions. 1. Commercially available bovine serum albumin as Cohn fraction V was demonstrated to contain small amounts of apolipoprotein-CII. 2. This apolipoprotein activated lipoprotein lipase in the same way as apolipoprotein-CII purified from human very-low-density lipoproteins."}
{"id": "PMID:477191", "title": "Inactive renin in rabbit plasma: effect of haemorrhage.", "content": "1. Renin activity in rabbit plasma increases after acidification (pH 3.3), probably due to activation of an inactive form of renin. 2. Both active and inactive renin in plasma increase after haemorrhage. This stimulus does not change the relative proportions of the two forms. 3. After ligation of the renal blood vessels neither form of renin increases in response to haemorrhage. 4. One day after bilateral nephrectomy no inactive renin could be demonstrated in plasma. 5. In the rabbit, therefore, the kidney is a major source of the inactive renin in plasma.", "contents": "Inactive renin in rabbit plasma: effect of haemorrhage. 1. Renin activity in rabbit plasma increases after acidification (pH 3.3), probably due to activation of an inactive form of renin. 2. Both active and inactive renin in plasma increase after haemorrhage. This stimulus does not change the relative proportions of the two forms. 3. After ligation of the renal blood vessels neither form of renin increases in response to haemorrhage. 4. One day after bilateral nephrectomy no inactive renin could be demonstrated in plasma. 5. In the rabbit, therefore, the kidney is a major source of the inactive renin in plasma."}
{"id": "PMID:477192", "title": "Hypertension with mineralocorticoid administration to the Long-Evans rat.", "content": "1. The Carworth Long-Evans rat has been reported to develop adrenal-regeneration hypertension but not deoxycorticosterone acetate (DOCA) hypertension. Deficiency of a hypothalamic receptor for deoxycorticosterone which mediates saline polydipsia has been postulated to underlie this resistance. Since a mineralocorticoid etiology for adrenal-regeneration hypertension has been postulated and all mineralocorticoids are thought to act on common receptors, these previous reports are difficult to reconcile. 2. To determine if an absolute or relative resistance to mineralocorticoids is present, Charles River Long-Evans and Sprague-Dawley rats were given 40 mg (107 micromol) of DOCA pellets/rat or 250 microgrms (0.65 micromol) of 2 alpha-methyl-9-alpha-fluorocortisol/day subcutaneously. 3. Saline polydipsia occurred with both steroids with both rat strains, though significantly less with the Long-Evans rats. Both types of rats became hypertensive and developed cardiac and renal enlargement with both steroids. Hypertension developed more rapidly with 2 alpha-methyl-9 alpha-fluorocortisol. 4. Thus mineralocorticoid hypertension can be produced in the Charles River Long-Evans rat, and the development of adrenal-regeneration hypertension in this rat strain is not incompatible with a mineralocorticoid etiology for adrenal-regeneration hypertension.", "contents": "Hypertension with mineralocorticoid administration to the Long-Evans rat. 1. The Carworth Long-Evans rat has been reported to develop adrenal-regeneration hypertension but not deoxycorticosterone acetate (DOCA) hypertension. Deficiency of a hypothalamic receptor for deoxycorticosterone which mediates saline polydipsia has been postulated to underlie this resistance. Since a mineralocorticoid etiology for adrenal-regeneration hypertension has been postulated and all mineralocorticoids are thought to act on common receptors, these previous reports are difficult to reconcile. 2. To determine if an absolute or relative resistance to mineralocorticoids is present, Charles River Long-Evans and Sprague-Dawley rats were given 40 mg (107 micromol) of DOCA pellets/rat or 250 microgrms (0.65 micromol) of 2 alpha-methyl-9-alpha-fluorocortisol/day subcutaneously. 3. Saline polydipsia occurred with both steroids with both rat strains, though significantly less with the Long-Evans rats. Both types of rats became hypertensive and developed cardiac and renal enlargement with both steroids. Hypertension developed more rapidly with 2 alpha-methyl-9 alpha-fluorocortisol. 4. Thus mineralocorticoid hypertension can be produced in the Charles River Long-Evans rat, and the development of adrenal-regeneration hypertension in this rat strain is not incompatible with a mineralocorticoid etiology for adrenal-regeneration hypertension."}
{"id": "PMID:477193", "title": "Evidence for activation of circulating inactive renin by the human kidney.", "content": "1. In eight patients with essential hypertension (EHT) and six patients with renovascular hypertension (RVHT) peripheral venous enzymatically active and inactive renin values were followed after acute stimulation of renin release by the vasodilating agent diazoxide (300 mg intravenously). Active renin rose during the first hour after diazoxide and remained high during the following 15 h, but inactive renin fell during the first hour and rose thereafter. Peripheral venous active and inactive renin were not different from arterial values both before and after diazoxide. 2. Sixteen patients with EHT received propranolol, 80 mg, four times a day. Six of them had a first injection of diazoxide the day before propranolol was started and a second one after 10--14 days of propranolol treatment. Peripheral vein active renin was lowered by propranolol, but inactive renin was raised. Both the diazoxide-induced rapid rise of active renin and the fall of inactive renin observed in untreated patients were absent during treatment with propranolol. 3. In four patients with EHT and seven patients with RVHT renal vein sampling was performed before and 30 min after diazoxide. Increased release of active renin from kidneys that were not markedly contracted was associated with a fall of the renal vein to peripheral vein ratio of inactive renin to a value less than one. 4. It is concluded that under certain circumstances stimulated release of active renin is associated with removal of inactive renin from the circulation by the kidney. This may in fact be due to intrarenal transformation of circulating inactive renin into its active counterpart. The findings suggest that a beta-adrenoreceptor might be involved in this activation process.", "contents": "Evidence for activation of circulating inactive renin by the human kidney. 1. In eight patients with essential hypertension (EHT) and six patients with renovascular hypertension (RVHT) peripheral venous enzymatically active and inactive renin values were followed after acute stimulation of renin release by the vasodilating agent diazoxide (300 mg intravenously). Active renin rose during the first hour after diazoxide and remained high during the following 15 h, but inactive renin fell during the first hour and rose thereafter. Peripheral venous active and inactive renin were not different from arterial values both before and after diazoxide. 2. Sixteen patients with EHT received propranolol, 80 mg, four times a day. Six of them had a first injection of diazoxide the day before propranolol was started and a second one after 10--14 days of propranolol treatment. Peripheral vein active renin was lowered by propranolol, but inactive renin was raised. Both the diazoxide-induced rapid rise of active renin and the fall of inactive renin observed in untreated patients were absent during treatment with propranolol. 3. In four patients with EHT and seven patients with RVHT renal vein sampling was performed before and 30 min after diazoxide. Increased release of active renin from kidneys that were not markedly contracted was associated with a fall of the renal vein to peripheral vein ratio of inactive renin to a value less than one. 4. It is concluded that under certain circumstances stimulated release of active renin is associated with removal of inactive renin from the circulation by the kidney. This may in fact be due to intrarenal transformation of circulating inactive renin into its active counterpart. The findings suggest that a beta-adrenoreceptor might be involved in this activation process."}
{"id": "PMID:477194", "title": "Structural and functional alterations in the mucosa of self-filling intestinal blind loops in rats.", "content": "1. Self-filling blind loops of jejunum were constructed in three groups of rats; in the first, blind loops were created without further manipulation; in the second the bile was diverted permanently into the lower ileum below the blind loop, whereas in a third neomycin was added to the drinking water throughout the experiment. Two weeks after the creation of the blind loops, they were used for structural and functional studies. 2. Morphometric and microdissection techniques demonstrated that the surface area of the individual villi of the mucosa of 'ordinary' blind loops had increased fourfold in comparison with corresponding control jejunum, whereas the increase was only twofold in rats with bile diversion or in the series treated with neomycin. There were proportional increases in crypt length and mitotoic activity of the crypts in all three series, which suggest that the alterations in the mucosa were due to hyperplasia in both villus and crypt compartments. 3. Sucrase, succinate dehydrogenase, alkaline phosphatase and non-specific esterase activities, determined biochemically or histochemically, were reduced in the mucosae of all blind loops, though the changes were most pronounced in the 'ordinary' blind loops. The accumulation of L-phenylalanine by mucosal slices in vitro was depressed, although the decrease was less marked in the series treated with neomycin. 4. These results suggest that both bacteria and deconjugated bile acids play a role in the development of the hyperplastic changes of the blind-loop mucosa, but that another factor might also be involved: as a possible candidate, stasis of the intestinal contents was considered. 5. To test this hypothesis, loops of rat colon were transposed into the jejunum. Above the transposed loop, the jejunal mucosa developed hyperplasia of both villus and crypt compartments, with a reduction in its ability to accumulate L-phenylalanine. It is argued that these changes, probably caused by stasis of the intestinal contents, are triggered off by the dilatation of the gut, which may also be implicated in the mucosal alterations in blind loops.", "contents": "Structural and functional alterations in the mucosa of self-filling intestinal blind loops in rats. 1. Self-filling blind loops of jejunum were constructed in three groups of rats; in the first, blind loops were created without further manipulation; in the second the bile was diverted permanently into the lower ileum below the blind loop, whereas in a third neomycin was added to the drinking water throughout the experiment. Two weeks after the creation of the blind loops, they were used for structural and functional studies. 2. Morphometric and microdissection techniques demonstrated that the surface area of the individual villi of the mucosa of 'ordinary' blind loops had increased fourfold in comparison with corresponding control jejunum, whereas the increase was only twofold in rats with bile diversion or in the series treated with neomycin. There were proportional increases in crypt length and mitotoic activity of the crypts in all three series, which suggest that the alterations in the mucosa were due to hyperplasia in both villus and crypt compartments. 3. Sucrase, succinate dehydrogenase, alkaline phosphatase and non-specific esterase activities, determined biochemically or histochemically, were reduced in the mucosae of all blind loops, though the changes were most pronounced in the 'ordinary' blind loops. The accumulation of L-phenylalanine by mucosal slices in vitro was depressed, although the decrease was less marked in the series treated with neomycin. 4. These results suggest that both bacteria and deconjugated bile acids play a role in the development of the hyperplastic changes of the blind-loop mucosa, but that another factor might also be involved: as a possible candidate, stasis of the intestinal contents was considered. 5. To test this hypothesis, loops of rat colon were transposed into the jejunum. Above the transposed loop, the jejunal mucosa developed hyperplasia of both villus and crypt compartments, with a reduction in its ability to accumulate L-phenylalanine. It is argued that these changes, probably caused by stasis of the intestinal contents, are triggered off by the dilatation of the gut, which may also be implicated in the mucosal alterations in blind loops."}
{"id": "PMID:477198", "title": "The role of the baroreceptor reflex in the cardiovascular effects of propranolol in the conscious spontaneously hypertensive rat.", "content": "1. The role of baroreceptors in the cardiovascular mechanism of action of DL-propranolol has been studied by comparing the acute effects of subcutaneous injection of 1 and 5 mg/kg (3.3 x 10(-6) and 16.5 x 10(-6) mol/kg) of this drug in conscious baroreceptor-denervated spontaneously hypertensive (SH) rats with those in sham-operated control SH rats. 2. At 5 mg/kg (16.5 x 10(-6) mol/kg) propranolol caused a small, but significant, increase in blood pressure in sham-operated SH rats, whereas both after 1 and 5 mg/kg (3.3 x 10(-6) and 16.5 x 10(-6) mol/kg) immediate hypotension was observed in baroreceptor-denervated animals. 3. Heart rate dropped rapidly after injection of 1 or 5 mg/kg (3.3 x 10(-6) and 16.5 x 10(-6) mol/kg) propranolol both in the baroreceptor-denervated and sham-operated SH rats. Bradycardia was significantly larger in the baroreceptor-denervated animals after an injection of 5 mg/kg (16.5 x 10(-6) mol/kg). 4. It is concluded that the lack of an early hypotensive effect of propranolol in intact animals is caused by an increased baroreceptor reflex activity as a consequence of the fall in cardiac output.", "contents": "The role of the baroreceptor reflex in the cardiovascular effects of propranolol in the conscious spontaneously hypertensive rat. 1. The role of baroreceptors in the cardiovascular mechanism of action of DL-propranolol has been studied by comparing the acute effects of subcutaneous injection of 1 and 5 mg/kg (3.3 x 10(-6) and 16.5 x 10(-6) mol/kg) of this drug in conscious baroreceptor-denervated spontaneously hypertensive (SH) rats with those in sham-operated control SH rats. 2. At 5 mg/kg (16.5 x 10(-6) mol/kg) propranolol caused a small, but significant, increase in blood pressure in sham-operated SH rats, whereas both after 1 and 5 mg/kg (3.3 x 10(-6) and 16.5 x 10(-6) mol/kg) immediate hypotension was observed in baroreceptor-denervated animals. 3. Heart rate dropped rapidly after injection of 1 or 5 mg/kg (3.3 x 10(-6) and 16.5 x 10(-6) mol/kg) propranolol both in the baroreceptor-denervated and sham-operated SH rats. Bradycardia was significantly larger in the baroreceptor-denervated animals after an injection of 5 mg/kg (16.5 x 10(-6) mol/kg). 4. It is concluded that the lack of an early hypotensive effect of propranolol in intact animals is caused by an increased baroreceptor reflex activity as a consequence of the fall in cardiac output."}
{"id": "PMID:477199", "title": "Renal sodium retention in cirrhosis: relation to aldosterone and nephron site.", "content": "1. In a group of patients with cirrhosis who showed a wide range of values for the rate of renal sodium excretion, the latter was found to be inversely related to both the plasma concentration and rate of renal excretion of aldosterone. However, for a given sodium excretion the values for aldosterone were significantly lower in the patients than for a group of healthy control subjects. These findings suggest either an increased renal tubular sensitivity to aldosterone or the participation of other factors in the pathogenesis of the sodium retention. 2. Based on measurements of the rate of urine flow and the clearances of free water and inulin during a maximal water diuresis, the fractional reabsorption of sodium by the 'proximal', 'diluting segment' and 'distal' segments of the nephron was estimated. For patients retaining sodium the enhanced reabsorption occurred at both proximal and distal sites, the latter being quantitatively more important. There was no significantly enhanced sodium reabsorption in the diluting segment.", "contents": "Renal sodium retention in cirrhosis: relation to aldosterone and nephron site. 1. In a group of patients with cirrhosis who showed a wide range of values for the rate of renal sodium excretion, the latter was found to be inversely related to both the plasma concentration and rate of renal excretion of aldosterone. However, for a given sodium excretion the values for aldosterone were significantly lower in the patients than for a group of healthy control subjects. These findings suggest either an increased renal tubular sensitivity to aldosterone or the participation of other factors in the pathogenesis of the sodium retention. 2. Based on measurements of the rate of urine flow and the clearances of free water and inulin during a maximal water diuresis, the fractional reabsorption of sodium by the 'proximal', 'diluting segment' and 'distal' segments of the nephron was estimated. For patients retaining sodium the enhanced reabsorption occurred at both proximal and distal sites, the latter being quantitatively more important. There was no significantly enhanced sodium reabsorption in the diluting segment."}
{"id": "PMID:477197", "title": "Myopathy in bone loss of ageing: improvement by treatment with 1 alpha-hydroxycholecalciferol and calcium.", "content": "1. Eleven patients with the bone loss of ageing were treated with the vitamin D analogue 1 alpha-hydroxycholecalciferol and calcium for 3--6 months. 2. Muscle biopsies were taken from the vastus lateralis before and after the treatment and the activity of several enzymes was measured. Succinate dehydrogenase and total phosphorylase activities, which are a measure of the oxidative capacity, were low and increased significantly with the treatment. The lactate dehydrogenase activity, which can be taken as a measure of the anaerobic metabolism, was normal and did not change with treatment. The phosphagen stores, ATP and creatine phosphate were low and increased to normal with treatment. 3. Histochemical classification of the fibre composition revealed that the treatment induced an increase in the relative number of fast-twitch a (FTa or type II A) fibres accompanied by a reduction of the fast-twitch b (FTb or type II B) fibres. The cross-sectional area of the FTa fibres also increased with the treatment. 4. The present findings indicate that treatment with the active vitamin D analogue, 1 alpha-hydroxycholecalciferol, and calcium improves the myopathy associated with the bone loss of ageing.", "contents": "Myopathy in bone loss of ageing: improvement by treatment with 1 alpha-hydroxycholecalciferol and calcium. 1. Eleven patients with the bone loss of ageing were treated with the vitamin D analogue 1 alpha-hydroxycholecalciferol and calcium for 3--6 months. 2. Muscle biopsies were taken from the vastus lateralis before and after the treatment and the activity of several enzymes was measured. Succinate dehydrogenase and total phosphorylase activities, which are a measure of the oxidative capacity, were low and increased significantly with the treatment. The lactate dehydrogenase activity, which can be taken as a measure of the anaerobic metabolism, was normal and did not change with treatment. The phosphagen stores, ATP and creatine phosphate were low and increased to normal with treatment. 3. Histochemical classification of the fibre composition revealed that the treatment induced an increase in the relative number of fast-twitch a (FTa or type II A) fibres accompanied by a reduction of the fast-twitch b (FTb or type II B) fibres. The cross-sectional area of the FTa fibres also increased with the treatment. 4. The present findings indicate that treatment with the active vitamin D analogue, 1 alpha-hydroxycholecalciferol, and calcium improves the myopathy associated with the bone loss of ageing."}
{"id": "PMID:477200", "title": "Pregnancy-associated disease of the renal microcirculation.", "content": "1. Platelet factor 3, platelet survival, renal platelet localization and fibrinolytic activity have been studied in normotensive subjects with a previous history of pregnancy-associated hypertension. Studies were carried out up to 6 years after the complicated pregnancy. 2. Renal platelet localization was found to be significantly increased despite normal platelet survival and platelet factor 3 availability. Significant impairment of both resting and post-venous-occlusion fibrinolytic activity was also demonstrable. 3. The significance of these findings in relation to mediation of progressive intrarenal vascular disease is discussed.", "contents": "Pregnancy-associated disease of the renal microcirculation. 1. Platelet factor 3, platelet survival, renal platelet localization and fibrinolytic activity have been studied in normotensive subjects with a previous history of pregnancy-associated hypertension. Studies were carried out up to 6 years after the complicated pregnancy. 2. Renal platelet localization was found to be significantly increased despite normal platelet survival and platelet factor 3 availability. Significant impairment of both resting and post-venous-occlusion fibrinolytic activity was also demonstrable. 3. The significance of these findings in relation to mediation of progressive intrarenal vascular disease is discussed."}
{"id": "PMID:477201", "title": "Carbohydrate-containing materials in urine from normal and diabetic subjects.", "content": "1. Abnormalities in glycoprotein metabolism are believed to play a role in diabetic microangiopathy. We have therefore measured the urinary excretion of carbohydrate-containing materials in normal and diabetic subjects. 2. Diabetic subjects were found to excrete excessive quantities of such material, which may parallel the increases observed in plasma and structural glycoproteins found in previous studies. 3. Systemic administration of a synthetic derivative of vasicine, which is known to affect mucus glycoprotein, was shown to restore these elevated urinary concentrations seen in diabetic subjects to values close to normal, but his drug had no significant effect on urinary concentrations of such materials in normal subjects.", "contents": "Carbohydrate-containing materials in urine from normal and diabetic subjects. 1. Abnormalities in glycoprotein metabolism are believed to play a role in diabetic microangiopathy. We have therefore measured the urinary excretion of carbohydrate-containing materials in normal and diabetic subjects. 2. Diabetic subjects were found to excrete excessive quantities of such material, which may parallel the increases observed in plasma and structural glycoproteins found in previous studies. 3. Systemic administration of a synthetic derivative of vasicine, which is known to affect mucus glycoprotein, was shown to restore these elevated urinary concentrations seen in diabetic subjects to values close to normal, but his drug had no significant effect on urinary concentrations of such materials in normal subjects."}
{"id": "PMID:477204", "title": "The pattern of breathing in patients with chronic airflow obstruction.", "content": "1. The pattern of breathing in 12 patients with severe irreversible airflow obstruction has been studied during ventilatory stimulation by rebreathing CO2. Mean maximum tidal volume response was only 1.23 +/- 0.30 litres (mean +/- SD); this represented 65% of mean measured vital capacity and 82% of mean measured inspiratory capacity. During the course of rebreathing mean total breath duration was reduced from 3.48 +/- 0.93 to 2.44 +/- 0.48 s. 2.  End-expiratory thoracic gas volume (FRC) was elevated at rest in all subjects and increased significantly by a further 0.50 +/- 1.90 litres during ventilatory stimulation in 10 of the 12 subjects. The maximum increase in FRC was proportional to the degree of airflow obstruction afforded by the airways in each subject. 3. It is suggested that the increase in FRC during ventilatory stimulation is responsible for the diminished tidal volume response and is an important determinant of breathing pattern and symptomatology in patients with airflow obstruction.", "contents": "The pattern of breathing in patients with chronic airflow obstruction. 1. The pattern of breathing in 12 patients with severe irreversible airflow obstruction has been studied during ventilatory stimulation by rebreathing CO2. Mean maximum tidal volume response was only 1.23 +/- 0.30 litres (mean +/- SD); this represented 65% of mean measured vital capacity and 82% of mean measured inspiratory capacity. During the course of rebreathing mean total breath duration was reduced from 3.48 +/- 0.93 to 2.44 +/- 0.48 s. 2.  End-expiratory thoracic gas volume (FRC) was elevated at rest in all subjects and increased significantly by a further 0.50 +/- 1.90 litres during ventilatory stimulation in 10 of the 12 subjects. The maximum increase in FRC was proportional to the degree of airflow obstruction afforded by the airways in each subject. 3. It is suggested that the increase in FRC during ventilatory stimulation is responsible for the diminished tidal volume response and is an important determinant of breathing pattern and symptomatology in patients with airflow obstruction."}
{"id": "PMID:477203", "title": "Diuretics, hepatic and thoracic duct lymph flows in the dog.", "content": "1. Simultaneous thoracic duct and hepatic lymph flows were measured in 29 mongrel dogs before and after the intravenous administration of mannitol, ethacrynic acid, frusemide and chlorothiazide in separate experiments. 2. Thoracic duct lymph flow increased significantly after each diuretic agent was administered. 3. Hepatic lymph flow increased only after ethacrynic acid and mannitol administration. Frusemide and chlorothiazide did not alter hepatic lymph flow. 4. These data show that increases in thoracic duct lymph flow after ethacrynic acid and mannitol arise partly from the liver, as well as from other organs.", "contents": "Diuretics, hepatic and thoracic duct lymph flows in the dog. 1. Simultaneous thoracic duct and hepatic lymph flows were measured in 29 mongrel dogs before and after the intravenous administration of mannitol, ethacrynic acid, frusemide and chlorothiazide in separate experiments. 2. Thoracic duct lymph flow increased significantly after each diuretic agent was administered. 3. Hepatic lymph flow increased only after ethacrynic acid and mannitol administration. Frusemide and chlorothiazide did not alter hepatic lymph flow. 4. These data show that increases in thoracic duct lymph flow after ethacrynic acid and mannitol arise partly from the liver, as well as from other organs."}
{"id": "PMID:477205", "title": "Erythropoiesis, iron stores and tissue iron exchange in man.", "content": "1. The exchange of iron between plasma and erythroid and non-erythroid tissues was measured in 14 normal subjects, 17 iron-deficient patients, nine patients with primary idiopathic haemochromatosis and in 13 patients with haemolytic disorders. 2. The two main factors determining tissue iron turnover were shown to be the level of iron stores and the erythropoietic activity of the bone marrow.", "contents": "Erythropoiesis, iron stores and tissue iron exchange in man. 1. The exchange of iron between plasma and erythroid and non-erythroid tissues was measured in 14 normal subjects, 17 iron-deficient patients, nine patients with primary idiopathic haemochromatosis and in 13 patients with haemolytic disorders. 2. The two main factors determining tissue iron turnover were shown to be the level of iron stores and the erythropoietic activity of the bone marrow."}
{"id": "PMID:477208", "title": "Plasma renin activity in diabetes mellitus.", "content": "1. The plasma renin activity (PRA) was measured in 76 diabetic patients who were attending an outpatients clinic. Of these patients 16 had untreated hypertension and 28 had diabetic complications, which ranged from microaneurysms to renal failure and blindness. 2. Compared with age- and sex-matched normotensive control subjects, both normotensive and hypertensive diabetic patients had significantly higher PRA (P less than 0.001). 3. Hypertensive diabetic patients also showed a higher PRA than matched hypertensive control subjects (P less than 0.005). There were no significant differences between diabetic patients with hypertension or complications compared with those without these features. 4. Although this elevation of PRA could be due to a change in another component of the renin-angiotensin system, hypersecretion of renin is the most likely explanation.", "contents": "Plasma renin activity in diabetes mellitus. 1. The plasma renin activity (PRA) was measured in 76 diabetic patients who were attending an outpatients clinic. Of these patients 16 had untreated hypertension and 28 had diabetic complications, which ranged from microaneurysms to renal failure and blindness. 2. Compared with age- and sex-matched normotensive control subjects, both normotensive and hypertensive diabetic patients had significantly higher PRA (P less than 0.001). 3. Hypertensive diabetic patients also showed a higher PRA than matched hypertensive control subjects (P less than 0.005). There were no significant differences between diabetic patients with hypertension or complications compared with those without these features. 4. Although this elevation of PRA could be due to a change in another component of the renin-angiotensin system, hypersecretion of renin is the most likely explanation."}
{"id": "PMID:477209", "title": "Plasma and urine dopamine in man given sodium chloride in the diet.", "content": "1. Plasma and urine free dopamine were measured daily for 5 days in six normal subjects maintained on a low sodium diet. The subjects were then given dietary supplements of sodium chloride for 5 days and the measurements repeated. 2. Throughout the experiment the 24 h free dopamine excretion rates for all the subjects were higher than could be accounted for by renal clearance. Dopamine excretion increased significantly in response to the added sodium chloride whereas plasma dopamine remained unchanged. The rise in dopamine excretion preceded that of sodium excretion. 3. It is concluded that free dopamine is formed within the kidney in response to increased dietary sodium and may have a role in the control of sodium excretion.", "contents": "Plasma and urine dopamine in man given sodium chloride in the diet. 1. Plasma and urine free dopamine were measured daily for 5 days in six normal subjects maintained on a low sodium diet. The subjects were then given dietary supplements of sodium chloride for 5 days and the measurements repeated. 2. Throughout the experiment the 24 h free dopamine excretion rates for all the subjects were higher than could be accounted for by renal clearance. Dopamine excretion increased significantly in response to the added sodium chloride whereas plasma dopamine remained unchanged. The rise in dopamine excretion preceded that of sodium excretion. 3. It is concluded that free dopamine is formed within the kidney in response to increased dietary sodium and may have a role in the control of sodium excretion."}
{"id": "PMID:477210", "title": "Sites of clearance of leucocyte pyrogen in the rabbit.", "content": "1. The dose-response curve for sustained infusions of leucocyte pyrogen has been demonstrated, and an optimum dose indicated for leucocyte pyrogen clearance experiments. 2. The lungs, liver and small bowel are not significantly involved in removal of leucocyte pyrogen from the circulation in conscious rabbits. 3. A single circulation through one kidney removes up to half of the infused dose of leucocyte pyrogen.", "contents": "Sites of clearance of leucocyte pyrogen in the rabbit. 1. The dose-response curve for sustained infusions of leucocyte pyrogen has been demonstrated, and an optimum dose indicated for leucocyte pyrogen clearance experiments. 2. The lungs, liver and small bowel are not significantly involved in removal of leucocyte pyrogen from the circulation in conscious rabbits. 3. A single circulation through one kidney removes up to half of the infused dose of leucocyte pyrogen."}
{"id": "PMID:477212", "title": "Correlation of ethanol concentrations in blood and saliva.", "content": "1. The concentrations of ethanol in blood, mixed saliva obtained before and after rinsing and drying the mouth and parotid saliva have been monitored in 12 healthy subjects after the ingestion of alcohol. 2. A highly significant linear correlation was found between blood and the three types of saliva examined from 20 min after completion of drinking. 3. Blood and mixed saliva samples were obtained from 20 patients attending the Casualty Department with evidence of ethanol intoxication. A similar correlation was obtained. 4. These results show that salivary ethanol may be used as an index of blood ethanol concentrations, provided that the salivary sample is not obtained within 20 min of the ingestion of alcohol.", "contents": "Correlation of ethanol concentrations in blood and saliva. 1. The concentrations of ethanol in blood, mixed saliva obtained before and after rinsing and drying the mouth and parotid saliva have been monitored in 12 healthy subjects after the ingestion of alcohol. 2. A highly significant linear correlation was found between blood and the three types of saliva examined from 20 min after completion of drinking. 3. Blood and mixed saliva samples were obtained from 20 patients attending the Casualty Department with evidence of ethanol intoxication. A similar correlation was obtained. 4. These results show that salivary ethanol may be used as an index of blood ethanol concentrations, provided that the salivary sample is not obtained within 20 min of the ingestion of alcohol."}
{"id": "PMID:477214", "title": "Plasma level and renal clearance of oxalate in normal subjects and in patients with primary hyperoxaluria or chronic renal failure or both.", "content": "1. Plasma oxalate has been measured by a radioisotopic method applicable to all concentrations of plasma oxalate and renal function, and also by an enzymatic method which was only applicable to raised concentrations of plasma oxalate. 2. Where the two methods could be applied simultaneously, the agreement between them was good. 3. Plasma oxalate was 86% ultrafiltrable at concentrations of up to 44 micromol/l. 4. Oxalate clearance and the exchangeable oxalate pool were also measured. The ratio of oxalate clearance to creatinine clearance was greater than unity in most normal subjects and patients. 5. These methods were used in normal subjects and in patients with primary hyperoxaluria and/or chronic renal failure. A raised plasma oxalate concentration was found in both conditions. Chronic renal failure is probably the most common cause of a raised plasma oxalate.", "contents": "Plasma level and renal clearance of oxalate in normal subjects and in patients with primary hyperoxaluria or chronic renal failure or both. 1. Plasma oxalate has been measured by a radioisotopic method applicable to all concentrations of plasma oxalate and renal function, and also by an enzymatic method which was only applicable to raised concentrations of plasma oxalate. 2. Where the two methods could be applied simultaneously, the agreement between them was good. 3. Plasma oxalate was 86% ultrafiltrable at concentrations of up to 44 micromol/l. 4. Oxalate clearance and the exchangeable oxalate pool were also measured. The ratio of oxalate clearance to creatinine clearance was greater than unity in most normal subjects and patients. 5. These methods were used in normal subjects and in patients with primary hyperoxaluria and/or chronic renal failure. A raised plasma oxalate concentration was found in both conditions. Chronic renal failure is probably the most common cause of a raised plasma oxalate."}
{"id": "PMID:477215", "title": "Effect of the exercise of seven consecutive days hill-walking on fluid homeostasis.", "content": "1. The effect of 7 consecutive days of strenuous exercise, hill-walking, on water balance and distribution was studied in five subjects. The exercise was preceded and followed by 3 control days. The diet was fixed throughout but water was allowed ad libitum. 2. Packed cell volume was measured daily. Serum electrolytes and arginine vasopressin were measured twice daily. Daily water, sodium and potassium balances were calculated. 3. During exercise there was a fall in packed cell volume, reaching a maximum of 11% by day 5 and a retention of sodium reaching a cumulative maximum of 358 mmol by day 6. During and immediately after exercise there was a retention of potassium, reaching a total of 120 mmol by day 3 after stopping exercise. 4. There was a loss of 650 ml of water on day 1 of exercise, followed by a modest retention reaching a cumulative maximum of 650 ml on day 5 of exercise. 5. Neither arginine vasopressin nor serum electrolyte concentrations were affected by exercise. 6. From the packed cell volume, sodium and water balances it was calculated that by day 5 of exercise there was an increase in plasma volume of .068 litre (22%), an increase in interstitial fluid volume of 2.0 litres (17%) and a decrease in intracellular fluid volume of 1.8 litres (8%). 7. These changes, together with the clinical observation of facial and ankle oedema during the experiemnt, suggest that continuous exercise may cause oedema and thus may be a factor in the aetiology of high-altitude oedema.", "contents": "Effect of the exercise of seven consecutive days hill-walking on fluid homeostasis. 1. The effect of 7 consecutive days of strenuous exercise, hill-walking, on water balance and distribution was studied in five subjects. The exercise was preceded and followed by 3 control days. The diet was fixed throughout but water was allowed ad libitum. 2. Packed cell volume was measured daily. Serum electrolytes and arginine vasopressin were measured twice daily. Daily water, sodium and potassium balances were calculated. 3. During exercise there was a fall in packed cell volume, reaching a maximum of 11% by day 5 and a retention of sodium reaching a cumulative maximum of 358 mmol by day 6. During and immediately after exercise there was a retention of potassium, reaching a total of 120 mmol by day 3 after stopping exercise. 4. There was a loss of 650 ml of water on day 1 of exercise, followed by a modest retention reaching a cumulative maximum of 650 ml on day 5 of exercise. 5. Neither arginine vasopressin nor serum electrolyte concentrations were affected by exercise. 6. From the packed cell volume, sodium and water balances it was calculated that by day 5 of exercise there was an increase in plasma volume of .068 litre (22%), an increase in interstitial fluid volume of 2.0 litres (17%) and a decrease in intracellular fluid volume of 1.8 litres (8%). 7. These changes, together with the clinical observation of facial and ankle oedema during the experiemnt, suggest that continuous exercise may cause oedema and thus may be a factor in the aetiology of high-altitude oedema."}
{"id": "PMID:477218", "title": "Central and peripheral circulation in relation to muscle-fibre composition in normo- and hyper-tensive man.", "content": "1. Fibre composition in the vastus lateralis muscle, and blood pressure, were determined in age-matched normotensive (n = 22) subjects and previously untreated patients (n = 19) with essential hypertension. 2. In all subjects the muscle fibres were classified, as fast-twitch (FT) and slow-twitch (ST), and blood pressure was recorded. Eleven patients and seven of the normotensive subjects participated in an extended haemodynamic study with intra-arterial pressure measurements and determinations of leg blood flow. 3. In both normo- and hyper-tensive subjects, ambulant and intra-arterial blood pressure and leg vascular resistance were negatively correlated to the percentage of ST fibres; no significant correlation was found to total leg blood flow. 4. The findings show that muscles with a high proportion of FT fibres have a higher resistance than muscles rich in ST fibres and suggest that the type of fibre in skeletal muscle might be of importance for the development of the hypertensive disease.", "contents": "Central and peripheral circulation in relation to muscle-fibre composition in normo- and hyper-tensive man. 1. Fibre composition in the vastus lateralis muscle, and blood pressure, were determined in age-matched normotensive (n = 22) subjects and previously untreated patients (n = 19) with essential hypertension. 2. In all subjects the muscle fibres were classified, as fast-twitch (FT) and slow-twitch (ST), and blood pressure was recorded. Eleven patients and seven of the normotensive subjects participated in an extended haemodynamic study with intra-arterial pressure measurements and determinations of leg blood flow. 3. In both normo- and hyper-tensive subjects, ambulant and intra-arterial blood pressure and leg vascular resistance were negatively correlated to the percentage of ST fibres; no significant correlation was found to total leg blood flow. 4. The findings show that muscles with a high proportion of FT fibres have a higher resistance than muscles rich in ST fibres and suggest that the type of fibre in skeletal muscle might be of importance for the development of the hypertensive disease."}
{"id": "PMID:477219", "title": "Age-dependent changes in the rate of myofibrillar protein degradation in humans as assessed by 3-methylhistidine and creatinine excretion.", "content": "1. Urinary excretion of 3-methylhistidine and creatinine have been measured in normal male and female humans ranging in age from pre-term neonates to 68 years to assess changes in the rate of muscle protein degradation and in muscle mass. 2. The 3-methylhistidine excretion by six adult men was measured before and after subjects were transferred to meat-free diets. It was established that a meat-free diet should be eaten for a minimum of 3 days before urine collection to eliminate exogenous sources of 3-methylhistidine. 3. The 3-methylhistidine/creatinine excretion ratio declined about twofold between normal full-term birth and maturity. The ratio in pre-term neonates was higher than for full-term neonates. 4. The variability of 3-methylhistidine/creatinine ratios between individual untime urine samples within a subject is similar to the variability between different subjects with total daily collections. 5. The 3-methylhistidine content of human muscle averaged 3.63 +/- 0.06 micromol/g for subjects aged between 4 and 65 years with protein accounting for 20.4 +/- 0.8% of muscle weight. These values are used to relate 3-methylhistidine excretion to muscle protein degradation. 6. It is concluded that control groups must be matched by age and sex to the group being examined. Where creatinine excretion is not perturbed it can be used as a reference base for comparisons of 3-methylhistidine excretion to indicate the average fractional degradation rates of muscle protein.", "contents": "Age-dependent changes in the rate of myofibrillar protein degradation in humans as assessed by 3-methylhistidine and creatinine excretion. 1. Urinary excretion of 3-methylhistidine and creatinine have been measured in normal male and female humans ranging in age from pre-term neonates to 68 years to assess changes in the rate of muscle protein degradation and in muscle mass. 2. The 3-methylhistidine excretion by six adult men was measured before and after subjects were transferred to meat-free diets. It was established that a meat-free diet should be eaten for a minimum of 3 days before urine collection to eliminate exogenous sources of 3-methylhistidine. 3. The 3-methylhistidine/creatinine excretion ratio declined about twofold between normal full-term birth and maturity. The ratio in pre-term neonates was higher than for full-term neonates. 4. The variability of 3-methylhistidine/creatinine ratios between individual untime urine samples within a subject is similar to the variability between different subjects with total daily collections. 5. The 3-methylhistidine content of human muscle averaged 3.63 +/- 0.06 micromol/g for subjects aged between 4 and 65 years with protein accounting for 20.4 +/- 0.8% of muscle weight. These values are used to relate 3-methylhistidine excretion to muscle protein degradation. 6. It is concluded that control groups must be matched by age and sex to the group being examined. Where creatinine excretion is not perturbed it can be used as a reference base for comparisons of 3-methylhistidine excretion to indicate the average fractional degradation rates of muscle protein."}
{"id": "PMID:477220", "title": "Increased turnover of muscle contractile proteins in Duchenne muscular dystrophy as assessed by 3-methylhistidine and creatinine excretion.", "content": "1. Myofibrillar protein degradation has been measured in patients with Duchenne muscular dystrophy, normal boys, adult males and Duchenne carriers by the rate of 3-methylhistidine excretion after transfer of subjects to a meat-free diet. 2. Although absolute rates of protein breakdown are lower in Duchenne patients, expression of the data to allow for differences in muscle mass gives fractional degradation rates 2--3 times higher than in age-matched controls. 3. Fractional rates of muscle protein synthesis are increased in the Duchenne patients to almost the same extent as protein breakdown. 4. Rates of muscle protein breakdown in obligate and presumed carriers of the Duchenne gene are not different from controls.", "contents": "Increased turnover of muscle contractile proteins in Duchenne muscular dystrophy as assessed by 3-methylhistidine and creatinine excretion. 1. Myofibrillar protein degradation has been measured in patients with Duchenne muscular dystrophy, normal boys, adult males and Duchenne carriers by the rate of 3-methylhistidine excretion after transfer of subjects to a meat-free diet. 2. Although absolute rates of protein breakdown are lower in Duchenne patients, expression of the data to allow for differences in muscle mass gives fractional degradation rates 2--3 times higher than in age-matched controls. 3. Fractional rates of muscle protein synthesis are increased in the Duchenne patients to almost the same extent as protein breakdown. 4. Rates of muscle protein breakdown in obligate and presumed carriers of the Duchenne gene are not different from controls."}
{"id": "PMID:477221", "title": "Acyl-coenzyme A--cholesterol acyltransferase activity in human liver.", "content": "1. In the presence of CoA and ATP, human liver microsomes catalyse the incorporation of [14C]oleate or [14C]cholesterol into cholesteryl oleate, thus demonstrating the presence of acyl-coenzyme A-cholesterol acyltransferase (cholesterol acyltransferase) in human liver. 2. The enzyme has properties similar to those of rat liver enzyme and with both the concentration of endogenous cholesterol in the microsomal fraction is adequate to support a constant initial rate of esterification. However, unlike the rat liver enzyme, the human cholesterol acyltransferase does not efficiently utilize added cholesterol as substrate. 3. The activity of cholesterol acyltransferase in human liver was 25% of that measured in rat liver under similar conditions of assay.", "contents": "Acyl-coenzyme A--cholesterol acyltransferase activity in human liver. 1. In the presence of CoA and ATP, human liver microsomes catalyse the incorporation of [14C]oleate or [14C]cholesterol into cholesteryl oleate, thus demonstrating the presence of acyl-coenzyme A-cholesterol acyltransferase (cholesterol acyltransferase) in human liver. 2. The enzyme has properties similar to those of rat liver enzyme and with both the concentration of endogenous cholesterol in the microsomal fraction is adequate to support a constant initial rate of esterification. However, unlike the rat liver enzyme, the human cholesterol acyltransferase does not efficiently utilize added cholesterol as substrate. 3. The activity of cholesterol acyltransferase in human liver was 25% of that measured in rat liver under similar conditions of assay."}
{"id": "PMID:477222", "title": "Changes in cholesterol metabolism with dietary cholesterol in children with familial hypercholesterolaemia.", "content": "1. Possible defects in cholesterol metabolism were sought in children with familial hypercholesterolaemia. 2. In nine affected children (eight heterozygotes and one homozygote) and in five healthy children, cholesterol synthesis and bile acid synthesis were determined from the excretion of steroids in the faeces during a low cholesterol diet. Cholesterol synthesis of 10.1 +/- 4.4 mg day-1 kg-1 in the hypercholesterolaemic children was similar to that in these and other normal children. Mean bile acid synthesis of 4.0 +/- 2.1 mg day-1 kg-1 also resembled normal values though three severely affected heterozygotes excreted substantially less. 3. The response to 4 weeks' additional 450 mg of dietary cholesterol/day led to variable changes in the plasma cholesterol and in the sterol balance. On average the affected children showed a rise in plasma cholesterol which resembled that in healthy subjects. The sterol balance fell in most, suggesting a reduction in cholesterol synthesis, which is the normal response to dietary cholesterol. 4. The response to dietary cholesterol was therefore at least qualitatively similar in the hypercholesterolaemic children to that reported in healthy subjects.", "contents": "Changes in cholesterol metabolism with dietary cholesterol in children with familial hypercholesterolaemia. 1. Possible defects in cholesterol metabolism were sought in children with familial hypercholesterolaemia. 2. In nine affected children (eight heterozygotes and one homozygote) and in five healthy children, cholesterol synthesis and bile acid synthesis were determined from the excretion of steroids in the faeces during a low cholesterol diet. Cholesterol synthesis of 10.1 +/- 4.4 mg day-1 kg-1 in the hypercholesterolaemic children was similar to that in these and other normal children. Mean bile acid synthesis of 4.0 +/- 2.1 mg day-1 kg-1 also resembled normal values though three severely affected heterozygotes excreted substantially less. 3. The response to 4 weeks' additional 450 mg of dietary cholesterol/day led to variable changes in the plasma cholesterol and in the sterol balance. On average the affected children showed a rise in plasma cholesterol which resembled that in healthy subjects. The sterol balance fell in most, suggesting a reduction in cholesterol synthesis, which is the normal response to dietary cholesterol. 4. The response to dietary cholesterol was therefore at least qualitatively similar in the hypercholesterolaemic children to that reported in healthy subjects."}
{"id": "PMID:477223", "title": "Prolactin in primary aldosteronism.", "content": "1. This study was done to examine the possibility that escape from the sodium retention produced by aldosterone may be associated with an inhibition of prolactin secretion. Plasma prolactin concentrations were determined in seven patients with adrenal adenoma and aldosteronism, before and after unilateral adrenalectomy, at a time when they were in balance on a sodium intake of 109 mmol/day. 2. After operation, plasma aldosterone was normal [before operation, 52.7 +/- 12.1 (supine), 64.6 +/- 9.1 ng/dl (upright); after operation, 6.8 +/- 1.5 (supine), 11.1 +/- 3.0 ng/dl (upright)], while plasma prolactin remained unchanged [before operation, 19.6 +/- 2.8 (supine), 15.5 +/- 3.3 ng/ml (upright); after operation, 23.8 +/- 2.3 (supine), 11.1 +/- 3.0 ng/ml (upright)]. 3. Our results do not support a role for prolactin in the renal response to aldosterone in man.", "contents": "Prolactin in primary aldosteronism. 1. This study was done to examine the possibility that escape from the sodium retention produced by aldosterone may be associated with an inhibition of prolactin secretion. Plasma prolactin concentrations were determined in seven patients with adrenal adenoma and aldosteronism, before and after unilateral adrenalectomy, at a time when they were in balance on a sodium intake of 109 mmol/day. 2. After operation, plasma aldosterone was normal [before operation, 52.7 +/- 12.1 (supine), 64.6 +/- 9.1 ng/dl (upright); after operation, 6.8 +/- 1.5 (supine), 11.1 +/- 3.0 ng/dl (upright)], while plasma prolactin remained unchanged [before operation, 19.6 +/- 2.8 (supine), 15.5 +/- 3.3 ng/ml (upright); after operation, 23.8 +/- 2.3 (supine), 11.1 +/- 3.0 ng/ml (upright)]. 3. Our results do not support a role for prolactin in the renal response to aldosterone in man."}
{"id": "PMID:477225", "title": "Absorption of inorganic phosphate in the human small intestine.", "content": "1. Intestinal phosphate absorption in human subjects was studied by the technique of triple lumen intestinal perfusion in vivo. 2. Ileal phosphate absorption increased as the intraluminal phosphate concentration was increased. 3. Ileal rates of phosphate absorption were lower at any given intraluminal phosphate concentration than previously described jejunal rates. Acidification of the ileal lumen did not increase phosphate absorption. 4. Phosphate absorption was shown in the jejunum to be dependent on the intraluminal sodium concentration. 5. Phosphate absorption in the human small intestine consists of at least two components, one directly proportional to water movement and the second apparently independent of water movement.", "contents": "Absorption of inorganic phosphate in the human small intestine. 1. Intestinal phosphate absorption in human subjects was studied by the technique of triple lumen intestinal perfusion in vivo. 2. Ileal phosphate absorption increased as the intraluminal phosphate concentration was increased. 3. Ileal rates of phosphate absorption were lower at any given intraluminal phosphate concentration than previously described jejunal rates. Acidification of the ileal lumen did not increase phosphate absorption. 4. Phosphate absorption was shown in the jejunum to be dependent on the intraluminal sodium concentration. 5. Phosphate absorption in the human small intestine consists of at least two components, one directly proportional to water movement and the second apparently independent of water movement."}
{"id": "PMID:477224", "title": "Studies on mineralocorticoid 'escape' in cirrhosis.", "content": "1. The mineralocorticoid 9 alpha-fluorohydrocortisone was given to 12 patients with cirrhosis without ascites. In seven an 'escape' from its sodium-retaining effects was observed, the other five continuing to retain sodium. 2. Changes in plasma renin activity (PRA) and inulin clearance (Cinulin) were used in the assessment of possible changes in the 'effective' extracellular fluid volume. PRA fell and Cinulin increased to a similar extent in each of the two groups of patients. The findings do not support the concept that the failure to show the mineralocorticoid escape in some patients with cirrhosis is due to a failure of expansion of the effective extracellular fluid volume. 3. Sodium reabsorption in the different segments of the nephron as estimated by clearance techniques under conditions of maximal water diuresis showed that the greatest changes to account for both mineralocorticoid escape and sodium retention were in the part of the nephron beyond the diluting segment.", "contents": "Studies on mineralocorticoid 'escape' in cirrhosis. 1. The mineralocorticoid 9 alpha-fluorohydrocortisone was given to 12 patients with cirrhosis without ascites. In seven an 'escape' from its sodium-retaining effects was observed, the other five continuing to retain sodium. 2. Changes in plasma renin activity (PRA) and inulin clearance (Cinulin) were used in the assessment of possible changes in the 'effective' extracellular fluid volume. PRA fell and Cinulin increased to a similar extent in each of the two groups of patients. The findings do not support the concept that the failure to show the mineralocorticoid escape in some patients with cirrhosis is due to a failure of expansion of the effective extracellular fluid volume. 3. Sodium reabsorption in the different segments of the nephron as estimated by clearance techniques under conditions of maximal water diuresis showed that the greatest changes to account for both mineralocorticoid escape and sodium retention were in the part of the nephron beyond the diluting segment."}
{"id": "PMID:477226", "title": "Arterial distensibility in normal and hypertensive man.", "content": "1. The pulse-wave velocity along the brachial radial artery was measured in 38 subjects and volume distensibility values were calculated. 2. Measurements made at each subject's resting blood pressure showed that those with high blood pressure had higher pulse-wave velocity values and hence less distensible arteries. 3. An acute rise in arterial distending pressure was accompanied by a rise in pulse-wave velocity. 4. When pulse-wave velocity was remeasured in all subjects at identical arterial distengin pressures, no difference was found between hypertensive and normal subjects. 5. It appears that poor distensibility of large arteries in hypertensive subjects is a consequence of the elevated distending pressures and not due to irreversible structural changes in the arterial wall.", "contents": "Arterial distensibility in normal and hypertensive man. 1. The pulse-wave velocity along the brachial radial artery was measured in 38 subjects and volume distensibility values were calculated. 2. Measurements made at each subject's resting blood pressure showed that those with high blood pressure had higher pulse-wave velocity values and hence less distensible arteries. 3. An acute rise in arterial distending pressure was accompanied by a rise in pulse-wave velocity. 4. When pulse-wave velocity was remeasured in all subjects at identical arterial distengin pressures, no difference was found between hypertensive and normal subjects. 5. It appears that poor distensibility of large arteries in hypertensive subjects is a consequence of the elevated distending pressures and not due to irreversible structural changes in the arterial wall."}
{"id": "PMID:477235", "title": "A defect in zinc uptake by jejunal biopsies in acrodermatitis enteropathica.", "content": "1. In a system in vitro, 65Zn accumulation by jejunal mucosal biopsies from patients with acrodermatitis enteropathica was found to be markedly reduced compared with controls. 2. We suggest that defective uptake of zinc by enterocytes is the primary abnormality responsible for the zinc deficiency underlying this disorder.", "contents": "A defect in zinc uptake by jejunal biopsies in acrodermatitis enteropathica. 1. In a system in vitro, 65Zn accumulation by jejunal mucosal biopsies from patients with acrodermatitis enteropathica was found to be markedly reduced compared with controls. 2. We suggest that defective uptake of zinc by enterocytes is the primary abnormality responsible for the zinc deficiency underlying this disorder."}
{"id": "PMID:477236", "title": "Faecal methylamine in normal and uraemic subjects.", "content": "1. A ninhydrin-reacting substance seen during ion-exchange chromatography of faecal dialysate obtained in vivo from normal and uraemic subjects has been isolated and identified as methylamine. 2. Faecal dialysate concentrations of methylamine were higher in uraemic subjects and were related to the degree of renal impairment (P less than 0.05). 3. Methylamine concentrations in faecal dialysate and centrifugate were not significantly different.", "contents": "Faecal methylamine in normal and uraemic subjects. 1. A ninhydrin-reacting substance seen during ion-exchange chromatography of faecal dialysate obtained in vivo from normal and uraemic subjects has been isolated and identified as methylamine. 2. Faecal dialysate concentrations of methylamine were higher in uraemic subjects and were related to the degree of renal impairment (P less than 0.05). 3. Methylamine concentrations in faecal dialysate and centrifugate were not significantly different."}
{"id": "PMID:477238", "title": "Analysis of 47Ca kinetics in normal subjects by means of a compartmental model with a non-exchangeable plasma calcium fraction.", "content": "1. 47Ca kinetic studies were performed in eight normal young adult males. Plasma, urinary and faecal stable and radioactive calcium and whole-body radioactivity were measured for up to 40 days after intravenous administration of the tracer. 2. The plasma radioactivity data could be fitted to the sum of four exponentials corresponding to a four-compartment model, but this model did not account for the consistently greater 47Ca specific radioactivities in plasma relative to those in urine at the same time in five of the subjects. 3. A non-exchangeable fraction of plasma calcium estimated as 5% of the mass in the plasma compartment was postulated to account for this finding. 4. An estimate of the insensible losses of calcium was obtained from the whole-body radioactivity counting data.", "contents": "Analysis of 47Ca kinetics in normal subjects by means of a compartmental model with a non-exchangeable plasma calcium fraction. 1. 47Ca kinetic studies were performed in eight normal young adult males. Plasma, urinary and faecal stable and radioactive calcium and whole-body radioactivity were measured for up to 40 days after intravenous administration of the tracer. 2. The plasma radioactivity data could be fitted to the sum of four exponentials corresponding to a four-compartment model, but this model did not account for the consistently greater 47Ca specific radioactivities in plasma relative to those in urine at the same time in five of the subjects. 3. A non-exchangeable fraction of plasma calcium estimated as 5% of the mass in the plasma compartment was postulated to account for this finding. 4. An estimate of the insensible losses of calcium was obtained from the whole-body radioactivity counting data."}
{"id": "PMID:477241", "title": "Accidental hypothermia and rewarming in dogs.", "content": "1. Twenty lightly anaesthetized dogs were cooled to 29 degrees C by cold-water immersion. Ventilation was spontaneous and the animals were allowed to shiver freely. Metabolic heat production and respiratory heat exchange were measured during rewarming. 2. The animals were divided into four groups each of five dogs and each group was rewarmed by a different technique. The control group was allowed to rewarm spontaneously; a second group was given warm (45-50 degrees C) fully humidified air to breathe in addition; a third group was rewarmed in a hot-water bath (42-44 degrees C) and the remaining group was given in muscle relaxant to abolish shivering and rewarmed by warm inspired air only. 3. The group rewarmed in hot water achieved normal core temperature most rapidly but there was no difference in the rewarming rates of the group rewarmed spontaneously and of the group given warm air to breathe in addition. 4. The group given a muscle relaxant and rewarmed with warm inspired air required 12 h to achieve the same core temperature as the shivering groups achieved in 2 h. Compared with the heat produced by shivering the amount of heat which it was possible to transfer across the respiratory tract was so small that it did not materially influence the rate of rewarming.", "contents": "Accidental hypothermia and rewarming in dogs. 1. Twenty lightly anaesthetized dogs were cooled to 29 degrees C by cold-water immersion. Ventilation was spontaneous and the animals were allowed to shiver freely. Metabolic heat production and respiratory heat exchange were measured during rewarming. 2. The animals were divided into four groups each of five dogs and each group was rewarmed by a different technique. The control group was allowed to rewarm spontaneously; a second group was given warm (45-50 degrees C) fully humidified air to breathe in addition; a third group was rewarmed in a hot-water bath (42-44 degrees C) and the remaining group was given in muscle relaxant to abolish shivering and rewarmed by warm inspired air only. 3. The group rewarmed in hot water achieved normal core temperature most rapidly but there was no difference in the rewarming rates of the group rewarmed spontaneously and of the group given warm air to breathe in addition. 4. The group given a muscle relaxant and rewarmed with warm inspired air required 12 h to achieve the same core temperature as the shivering groups achieved in 2 h. Compared with the heat produced by shivering the amount of heat which it was possible to transfer across the respiratory tract was so small that it did not materially influence the rate of rewarming."}
{"id": "PMID:477243", "title": "Vitamin D and magnesium absorption in man.", "content": "1. The effects of vitamin D and its hydroxylated derivatives on calcium and magnesium absorption have been examined in 47 balance studies on patients with various disorders of calcium or bone metabolism. 2. Vitamin D significantly increased the mean net absorption of calcium and also the calcium balance. The mean net absorption of magnesium was also significantly increased although the rise was much less than that of calcium and the mean magnesium balance was unaffected. 3. It is suggested that the slight effect of vitamin D on magnesium absorption may be incidental to its stimulation of active calcium transport, since the latter system has weak affinities for other alkaline earth ions including strontium, barium and magnesium.", "contents": "Vitamin D and magnesium absorption in man. 1. The effects of vitamin D and its hydroxylated derivatives on calcium and magnesium absorption have been examined in 47 balance studies on patients with various disorders of calcium or bone metabolism. 2. Vitamin D significantly increased the mean net absorption of calcium and also the calcium balance. The mean net absorption of magnesium was also significantly increased although the rise was much less than that of calcium and the mean magnesium balance was unaffected. 3. It is suggested that the slight effect of vitamin D on magnesium absorption may be incidental to its stimulation of active calcium transport, since the latter system has weak affinities for other alkaline earth ions including strontium, barium and magnesium."}
{"id": "PMID:477245", "title": "Neurogenic activity--angiotensin II interaction during the development and maintenance of renal hypertension in the rat.", "content": "1. Pentolinium tartrate (a ganglionic blocker) was injected in conscious rats during the early and late phases of two-kidney renal hypertension produced by aortic ligation. 2. In the early phase ( 5 days after aortic ligation), ganglionic blockade resulted in a decrease in blood pressure equal to that obtained in normotensive rats. Later, at days 12 and 40, for equally severe hypertension, ganglion blockade resulted in a greater decrease in blood pressure. 3. A 30 min infusion of [Sar1, Ala8]angiotensin II during the pentolinium-induced nadir in blood pressure resulted in a further decrease in blood pressure at day 5. Later, at days 12 and 40, this effect was smaller. 4. A 300 min infusion of [Sar1, Ala8]angiotensin II normalized the blood pressure in hypertensive rats at day 40. This delay response may be secondary to a central effect of the antagonist, reducing neurogenic tone or peripheral antagonism of locally generated angiotensin II in the blood vessel walls. 5. At day 40, removal of the small left kidney resulted in a greater decrease in blood pressure. This suggests the presence of a renal factor other than renin in the chronic phase of this hypertension.", "contents": "Neurogenic activity--angiotensin II interaction during the development and maintenance of renal hypertension in the rat. 1. Pentolinium tartrate (a ganglionic blocker) was injected in conscious rats during the early and late phases of two-kidney renal hypertension produced by aortic ligation. 2. In the early phase ( 5 days after aortic ligation), ganglionic blockade resulted in a decrease in blood pressure equal to that obtained in normotensive rats. Later, at days 12 and 40, for equally severe hypertension, ganglion blockade resulted in a greater decrease in blood pressure. 3. A 30 min infusion of [Sar1, Ala8]angiotensin II during the pentolinium-induced nadir in blood pressure resulted in a further decrease in blood pressure at day 5. Later, at days 12 and 40, this effect was smaller. 4. A 300 min infusion of [Sar1, Ala8]angiotensin II normalized the blood pressure in hypertensive rats at day 40. This delay response may be secondary to a central effect of the antagonist, reducing neurogenic tone or peripheral antagonism of locally generated angiotensin II in the blood vessel walls. 5. At day 40, removal of the small left kidney resulted in a greater decrease in blood pressure. This suggests the presence of a renal factor other than renin in the chronic phase of this hypertension."}
{"id": "PMID:477250", "title": "A comparison of chromium sesquioxide and [51Cr]chromic chloride as inert markers in calcium balance studies.", "content": "1. Chromium sesquioxide (Cr2O3; 1.5 g/day) and [51Cr]chromic chloride [51CrCl3 (0.3 muCi/day)] were compared as continuously administered non-absorbed markers for the correction of faecal recoveries in 14 calcium balance studies each lasting at least 18 days. 2. The mean recoveries of each, 98.4% for Cr2O3 and 101.9% for 51CrCl3, were not significantly different from 100%. 3. The two markers reduced the uncertainity in a typical 3 x 6 day calcium balance study to a similar extent (SD = 1.4 mmol/day for Cr2O3 and SD = 1.5 mmol/day for 51CrCl3. 4. 51CrCl3 is a convenient and satisfactorily alternative to Cr2O3 when the laboratory hazards associated with estimating the latter cannot easily be eliminated.", "contents": "A comparison of chromium sesquioxide and [51Cr]chromic chloride as inert markers in calcium balance studies. 1. Chromium sesquioxide (Cr2O3; 1.5 g/day) and [51Cr]chromic chloride [51CrCl3 (0.3 muCi/day)] were compared as continuously administered non-absorbed markers for the correction of faecal recoveries in 14 calcium balance studies each lasting at least 18 days. 2. The mean recoveries of each, 98.4% for Cr2O3 and 101.9% for 51CrCl3, were not significantly different from 100%. 3. The two markers reduced the uncertainity in a typical 3 x 6 day calcium balance study to a similar extent (SD = 1.4 mmol/day for Cr2O3 and SD = 1.5 mmol/day for 51CrCl3. 4. 51CrCl3 is a convenient and satisfactorily alternative to Cr2O3 when the laboratory hazards associated with estimating the latter cannot easily be eliminated."}
{"id": "PMID:477252", "title": "Measurement of water distribution and transcapillary solute flux in dog lung by external radioactivity counting.", "content": "1. A method for measuring the pulmonary water compartments in vivo in anaesthetized dogs is described. 2. The technique depends upon the sequential intravenous injection of markers labelled with gamma-ray-emitting isotopes. Each is allowed to become evenly distributed throughout the compartment to which it is confined. A calibrated gamma-counter placed at the surface of the inflated lung then records a count rate delated to the total quantity of isotope in unit volume of tissue. The size of each water compartment can be calculated from the external count rate and the isotopic concentration of the marker in plasma. 3. The markers used were 125I-labelled serum albumin for plasma, [125I]iodide for extracellular water and [125I]iodoantipyrine for total water. 4. The method was extended to calculate trans-capillary flux rates of serum albumin and showed that rapid exchange of serum albumin takes place between the plasma and interstitial space. 5. Results obtained non-invasively by the isotopic method were compared with direct measurements. Agreement was good.", "contents": "Measurement of water distribution and transcapillary solute flux in dog lung by external radioactivity counting. 1. A method for measuring the pulmonary water compartments in vivo in anaesthetized dogs is described. 2. The technique depends upon the sequential intravenous injection of markers labelled with gamma-ray-emitting isotopes. Each is allowed to become evenly distributed throughout the compartment to which it is confined. A calibrated gamma-counter placed at the surface of the inflated lung then records a count rate delated to the total quantity of isotope in unit volume of tissue. The size of each water compartment can be calculated from the external count rate and the isotopic concentration of the marker in plasma. 3. The markers used were 125I-labelled serum albumin for plasma, [125I]iodide for extracellular water and [125I]iodoantipyrine for total water. 4. The method was extended to calculate trans-capillary flux rates of serum albumin and showed that rapid exchange of serum albumin takes place between the plasma and interstitial space. 5. Results obtained non-invasively by the isotopic method were compared with direct measurements. Agreement was good."}
{"id": "PMID:477251", "title": "Fractured neck of femur in the elderly: an attempt to identify patients at risk.", "content": "1. During a 15-month period, 110 elderly patients admitted with fractured neck of femur were studied in comparison with 72 elderly control patients undergoing elective surgery admitted over the same period to the same orthopaedic wards. 2. A striking finding was the marked similarity of all variables measured in the fracture and control groups. The principal differences between the two populations were that the fracture patients had a lower mean forearm bone mineral content, and that their serum concentrations of albumin, globulin and phosphate were reduced. 3. Neither measurements of radioisotopic calcium absorption, nor those of the serum concentrations of calcium, 25-hydroxy-vitamin D3 nor alkaline phosphatase provided satisfactory discrimination between the groups. 4. Several indices were devised, based on linear combinations of the test results obtained, in an attempt to predict the liability to future fractures of patients being considered for prophylactic therapy with oestrogens or other drugs. However, further work is required to define an index of improved predictive power and to evaluate it prospectively.", "contents": "Fractured neck of femur in the elderly: an attempt to identify patients at risk. 1. During a 15-month period, 110 elderly patients admitted with fractured neck of femur were studied in comparison with 72 elderly control patients undergoing elective surgery admitted over the same period to the same orthopaedic wards. 2. A striking finding was the marked similarity of all variables measured in the fracture and control groups. The principal differences between the two populations were that the fracture patients had a lower mean forearm bone mineral content, and that their serum concentrations of albumin, globulin and phosphate were reduced. 3. Neither measurements of radioisotopic calcium absorption, nor those of the serum concentrations of calcium, 25-hydroxy-vitamin D3 nor alkaline phosphatase provided satisfactory discrimination between the groups. 4. Several indices were devised, based on linear combinations of the test results obtained, in an attempt to predict the liability to future fractures of patients being considered for prophylactic therapy with oestrogens or other drugs. However, further work is required to define an index of improved predictive power and to evaluate it prospectively."}
{"id": "PMID:477254", "title": "Evidence for the local occurrence of angiotensin II in rat kidney and its modulation by dietary sodium intake and converting enzyme blockade.", "content": "1. Angiotensin II (ANG II) was measured in acid-ethanol homogenates of rapidly frozen rat kidneys by a method involving ion-exchange and immunoadsorbent purification of peptides before radioimmunoassay. 2. Concentrations of ANG II found in kidney were 10--20 times that in plasma. 3. Perfusion of the kidneys via the renal artery with isotonic sodium chloride solution or with disodium EDTA solution did not alter the concentrations of intrarenal ANG II. 4. Animals fed on a sodium-deficient diet for 8 days had markedly higher concentrations of intrarenal ANG II, plasma renin activity and kidney renin concentration than sodium-replete animals. 5. After oral sodium loading for 3 weeks, rats had suppressed plasma renin activity and kidney renin concentration but unchanged intrarenal ANG II when compared with animals on a normal sodium intake. 6. One hour after the administration of a converting enzyme inhibitor (SQ 20881) plasma renin activity was elevated, kidney renin concentration unchanged and intrarenal ANG II was depressed. 7. These results demonstrate the presence of ANG II in the extravascular compartment of the kidney. They further suggest that its quantity is influenced by sodium intake and that angiotensin I converting enzyme is essential for its formation.", "contents": "Evidence for the local occurrence of angiotensin II in rat kidney and its modulation by dietary sodium intake and converting enzyme blockade. 1. Angiotensin II (ANG II) was measured in acid-ethanol homogenates of rapidly frozen rat kidneys by a method involving ion-exchange and immunoadsorbent purification of peptides before radioimmunoassay. 2. Concentrations of ANG II found in kidney were 10--20 times that in plasma. 3. Perfusion of the kidneys via the renal artery with isotonic sodium chloride solution or with disodium EDTA solution did not alter the concentrations of intrarenal ANG II. 4. Animals fed on a sodium-deficient diet for 8 days had markedly higher concentrations of intrarenal ANG II, plasma renin activity and kidney renin concentration than sodium-replete animals. 5. After oral sodium loading for 3 weeks, rats had suppressed plasma renin activity and kidney renin concentration but unchanged intrarenal ANG II when compared with animals on a normal sodium intake. 6. One hour after the administration of a converting enzyme inhibitor (SQ 20881) plasma renin activity was elevated, kidney renin concentration unchanged and intrarenal ANG II was depressed. 7. These results demonstrate the presence of ANG II in the extravascular compartment of the kidney. They further suggest that its quantity is influenced by sodium intake and that angiotensin I converting enzyme is essential for its formation."}
{"id": "PMID:477256", "title": "Cysteine-homocysteine mixed disulphide: differing plasma concentrations in normal men and women.", "content": "1. The mixed disulphide of cysteine and homocysteine is known always to be present in the plasma of patients with homocystinuria, an abnormality of methionine metabolism. Recently we have shown that it is also detectable in low concentration in the plasma of normal fasting man. In the present study we measured mixed disulphide concentrations after an overnight fast in 24 normal men and compared the findings with those obtained in 24 normal premenopausal women of similar age. 2. The mean value for men (+/- SD) of 3.3 +/- 0.8 micromol/l was significantly higher than that for women (2.4 +/- 0.7 micromol/l; P less than 0.001). Of the other neutral and acidic amino acids measured mean values for leucine, isoleucine and valine (P less than 0.001) and cystine (P less than 0.01) were also higher in the men but methionine concentrations were not significantly different. 3. The higher branched-chain amino acid concentrations in men could be related to larger muscle bulk and protein intake, but the higher cysteine-homocysteine mixed disulphide concentrations are consistent with differences in methionine metabolism between men and women under the age of 50 years.", "contents": "Cysteine-homocysteine mixed disulphide: differing plasma concentrations in normal men and women. 1. The mixed disulphide of cysteine and homocysteine is known always to be present in the plasma of patients with homocystinuria, an abnormality of methionine metabolism. Recently we have shown that it is also detectable in low concentration in the plasma of normal fasting man. In the present study we measured mixed disulphide concentrations after an overnight fast in 24 normal men and compared the findings with those obtained in 24 normal premenopausal women of similar age. 2. The mean value for men (+/- SD) of 3.3 +/- 0.8 micromol/l was significantly higher than that for women (2.4 +/- 0.7 micromol/l; P less than 0.001). Of the other neutral and acidic amino acids measured mean values for leucine, isoleucine and valine (P less than 0.001) and cystine (P less than 0.01) were also higher in the men but methionine concentrations were not significantly different. 3. The higher branched-chain amino acid concentrations in men could be related to larger muscle bulk and protein intake, but the higher cysteine-homocysteine mixed disulphide concentrations are consistent with differences in methionine metabolism between men and women under the age of 50 years."}
{"id": "PMID:477257", "title": "Protein synthesis in isolated human skeletal muscle tissue: evaluation of an experimental model.", "content": "Amino acids were incorporated into soluble proteins, myosin and myoglobin at constant rates for at least 4 h on incubation of isolated human skeletal muscle fibres. The time course of incorporation of leucine into proteins not attached to ribosomes was approximately constant, suggesting a continuous termination of proteins. A comparison between estimated pool size of ribosomes and incorporation rate of leucine into proteins indicated that initiation of peptides occurred even in the absence of insulin.", "contents": "Protein synthesis in isolated human skeletal muscle tissue: evaluation of an experimental model. Amino acids were incorporated into soluble proteins, myosin and myoglobin at constant rates for at least 4 h on incubation of isolated human skeletal muscle fibres. The time course of incorporation of leucine into proteins not attached to ribosomes was approximately constant, suggesting a continuous termination of proteins. A comparison between estimated pool size of ribosomes and incorporation rate of leucine into proteins indicated that initiation of peptides occurred even in the absence of insulin."}
{"id": "PMID:477258", "title": "Sodium excretion in man, and adaptation to a low-sodium diet: effect of intravenous sodium chloride.", "content": "1. The aim of this study was to test whether a postulated gastrointestinal or portal monitor of sodium intake plays any part in adjusting renal sodium excretion when dietary sodium is reduced. 2. Normal male subjects were given 50 mmol of sodium chloride intravenously three times daily for 3 days to replace or to supplement a constant oral intake of sodium chloride. 3. When oral sodium chloride was replaced with intravenous sodium chloride, renal sodium excretion remained constant. 4. When oral sodium chloride was kept constant, sodium administered as intravenous sodium chloride was promptly excreted in three out of four subjects. There was a delay in the increase in sodium excretion in the fourth subject. 5. Infusions containing 50 mmol of sodium chloride in 50 ml given intravenously over 22 min produced a rise in plasma sodium concentration and a fall in concentration of total plasma solids. 6. These results provide no evidence for a gastrointestinal or portal monitor of sodium intake, but do not disprove the existence of such a monitor.", "contents": "Sodium excretion in man, and adaptation to a low-sodium diet: effect of intravenous sodium chloride. 1. The aim of this study was to test whether a postulated gastrointestinal or portal monitor of sodium intake plays any part in adjusting renal sodium excretion when dietary sodium is reduced. 2. Normal male subjects were given 50 mmol of sodium chloride intravenously three times daily for 3 days to replace or to supplement a constant oral intake of sodium chloride. 3. When oral sodium chloride was replaced with intravenous sodium chloride, renal sodium excretion remained constant. 4. When oral sodium chloride was kept constant, sodium administered as intravenous sodium chloride was promptly excreted in three out of four subjects. There was a delay in the increase in sodium excretion in the fourth subject. 5. Infusions containing 50 mmol of sodium chloride in 50 ml given intravenously over 22 min produced a rise in plasma sodium concentration and a fall in concentration of total plasma solids. 6. These results provide no evidence for a gastrointestinal or portal monitor of sodium intake, but do not disprove the existence of such a monitor."}
{"id": "PMID:477259", "title": "Changes in blood glucose and plasma insulin after intravenous galactose in human injury.", "content": "1. The increase in blood glucose after intravenous galactose was measured at 6--8 h and 14 days after hip replacement (14 patients) and accidental injury (14 patients). 2. After hip replacement there was a greater rise of glucose after galactose on the day of the operation than on recovery, despite basal hyperglycaemia. This earlier period was also associated with inappropriately low insulin concentrations for the prevailing glucose concentration, and hyperketonaemia (13 out 14 patients). 3. After accidental injury patients with initial hyperketonaemia (nine out of 14) also had a greater rise of blood glucose after galactose than on recovery and had relative insulin deficiency; in contrast those who were initially normoketonaemic (five out of 14) showed a rise in glucose comparable with that after recovery and basal insulin concentrations more appropriate to the existing glucose concentration. 4. It is concluded that in most subjects after injury hepatic glucose release after galactose administration is not suppressed despite hyperglycaemia.", "contents": "Changes in blood glucose and plasma insulin after intravenous galactose in human injury. 1. The increase in blood glucose after intravenous galactose was measured at 6--8 h and 14 days after hip replacement (14 patients) and accidental injury (14 patients). 2. After hip replacement there was a greater rise of glucose after galactose on the day of the operation than on recovery, despite basal hyperglycaemia. This earlier period was also associated with inappropriately low insulin concentrations for the prevailing glucose concentration, and hyperketonaemia (13 out 14 patients). 3. After accidental injury patients with initial hyperketonaemia (nine out of 14) also had a greater rise of blood glucose after galactose than on recovery and had relative insulin deficiency; in contrast those who were initially normoketonaemic (five out of 14) showed a rise in glucose comparable with that after recovery and basal insulin concentrations more appropriate to the existing glucose concentration. 4. It is concluded that in most subjects after injury hepatic glucose release after galactose administration is not suppressed despite hyperglycaemia."}
{"id": "PMID:477260", "title": "Effect of age on hypertensive stimuli and the development of hypertension in Brattleboro rats.", "content": "1. In rats with inherited diabetes insipidus, unilateral nephrectomy plus drinking of 0.6% NaCl solution (saline) did not influence blood pressure in adult rats. However, when these factors applied before puberty, they produced hypertension. 2. We therefore analysed whether saline administration before puberty or unilateral nephrectomy before puberty was more important for this hypertensive response. 3. Saline drinking was found to be necessary for the response because hypertension was elicited by unilateral nephrectomy in adult rats only if saline consumption began before puberty.", "contents": "Effect of age on hypertensive stimuli and the development of hypertension in Brattleboro rats. 1. In rats with inherited diabetes insipidus, unilateral nephrectomy plus drinking of 0.6% NaCl solution (saline) did not influence blood pressure in adult rats. However, when these factors applied before puberty, they produced hypertension. 2. We therefore analysed whether saline administration before puberty or unilateral nephrectomy before puberty was more important for this hypertensive response. 3. Saline drinking was found to be necessary for the response because hypertension was elicited by unilateral nephrectomy in adult rats only if saline consumption began before puberty."}
{"id": "PMID:477261", "title": "Peptiduria in experimental Fanconi syndrome in rats.", "content": "1. The plasma concentrations and urinary output of proline and hydroxyproline contained in peptides were measured in normal rats and in rats with Fanconi syndrome produced by injection of sodium maleate. All animals received a prior injection of 10 mg of the dipeptide L-prolyl-L-hydroxy-proline to increase plasma and urinary peptide content. There was a significant increase of urinary output of the two imino acids contained in peptides and a fall in their plasma concentrations. 2. It is concluded that increased output of peptides derived from collagen degradation in the experimental Fanconi syndrome in rats is at least in part due to diminished tubular reabsorption of these compounds from the glomerular filtrate. The results are claimed to be relevant to the increased output of urinary peptides in the Fanconi syndrome in man.", "contents": "Peptiduria in experimental Fanconi syndrome in rats. 1. The plasma concentrations and urinary output of proline and hydroxyproline contained in peptides were measured in normal rats and in rats with Fanconi syndrome produced by injection of sodium maleate. All animals received a prior injection of 10 mg of the dipeptide L-prolyl-L-hydroxy-proline to increase plasma and urinary peptide content. There was a significant increase of urinary output of the two imino acids contained in peptides and a fall in their plasma concentrations. 2. It is concluded that increased output of peptides derived from collagen degradation in the experimental Fanconi syndrome in rats is at least in part due to diminished tubular reabsorption of these compounds from the glomerular filtrate. The results are claimed to be relevant to the increased output of urinary peptides in the Fanconi syndrome in man."}
{"id": "PMID:477287", "title": "Bedside maneuvers in the diagnosis of heart disease.", "content": "Numerous other pathologic conditions are encountered. The relatively few maneuvers and drugs presented here were chosen as the ones most commonly used in daily practice. Cardiac auscultation is just one aspect of the cardiovascular examination; equal attention should be paid to determination of jugular venous pressure, jugular venous wave form, carotid upstroke characteristics, and to observation and palpation of the precordium. Finally, if the examiner keeps in mind the hemodynamic changes induced by the various maneuvers and pharmacologic agents while auscultating the heart, and if he understands the pathophysiology of the commonly encountered cardiac lesions, he should be able to diagnose heart disease and to evaluate more intelligently various murmurs and heart sounds without necessitating the use of expensive invasive or noninvasive procedures.", "contents": "Bedside maneuvers in the diagnosis of heart disease. Numerous other pathologic conditions are encountered. The relatively few maneuvers and drugs presented here were chosen as the ones most commonly used in daily practice. Cardiac auscultation is just one aspect of the cardiovascular examination; equal attention should be paid to determination of jugular venous pressure, jugular venous wave form, carotid upstroke characteristics, and to observation and palpation of the precordium. Finally, if the examiner keeps in mind the hemodynamic changes induced by the various maneuvers and pharmacologic agents while auscultating the heart, and if he understands the pathophysiology of the commonly encountered cardiac lesions, he should be able to diagnose heart disease and to evaluate more intelligently various murmurs and heart sounds without necessitating the use of expensive invasive or noninvasive procedures."}
{"id": "PMID:477297", "title": "Religious beliefs and psychiatric disorder in Sri Lanka.", "content": "After a brief description of the popular beliefs on the causation of illness held in Sir Lanka, eight illustrative case histories are presented. Although the incidence of religious-supernatural contents of mental disorders has been decreasing in the West it is by no means rare. It does, however, occur mostly in psychotic illness, whereas in Sri Lanka it occurs with great frequency in both psychotic and neurotic conditions. The indigenous exorcistic methods of treatment will be more effective in some cases than in others and a careful diagnosis will provide indications as to the choice of treatment.", "contents": "Religious beliefs and psychiatric disorder in Sri Lanka. After a brief description of the popular beliefs on the causation of illness held in Sir Lanka, eight illustrative case histories are presented. Although the incidence of religious-supernatural contents of mental disorders has been decreasing in the West it is by no means rare. It does, however, occur mostly in psychotic illness, whereas in Sri Lanka it occurs with great frequency in both psychotic and neurotic conditions. The indigenous exorcistic methods of treatment will be more effective in some cases than in others and a careful diagnosis will provide indications as to the choice of treatment."}
{"id": "PMID:477298", "title": "Further data on the diagnostic value of spontaneous drawing.", "content": "In discussing 2 patients, the authors point out that a study of spontaneous drawings by psychotics can be of considerable diagnostic importance in clinical practice. The clear structure of the drawings threw doubt on the diagnosis of schizophrenia in the case of the first patient who could be classified in the schizophenia spectrum on the basis of the formal symptoms. In the case of the second patient, where th clinical picture suggested different diagnosis, the drawings represent the 'ossified' essence of the experience and behavior symptoms and thus provided an objective basis for nosological classification. In addition, a characteristic feature of the difference between the creativity of the schizophrenic and the creative artist could be seen from a comparison with medieval Gothic art.", "contents": "Further data on the diagnostic value of spontaneous drawing. In discussing 2 patients, the authors point out that a study of spontaneous drawings by psychotics can be of considerable diagnostic importance in clinical practice. The clear structure of the drawings threw doubt on the diagnosis of schizophrenia in the case of the first patient who could be classified in the schizophenia spectrum on the basis of the formal symptoms. In the case of the second patient, where th clinical picture suggested different diagnosis, the drawings represent the 'ossified' essence of the experience and behavior symptoms and thus provided an objective basis for nosological classification. In addition, a characteristic feature of the difference between the creativity of the schizophrenic and the creative artist could be seen from a comparison with medieval Gothic art."}
{"id": "PMID:477300", "title": "[Word play and schizophrenic language disorder - what constitutes the difference?].", "content": "The listener often perceives a similarity between humorous comments by schizophrenic patients and linguistic word plays, especially puns. A structural analysis of specific examples illustrates the basic difference. In 'good' as well as 'bad' word plays the humorous effect stems from the surprising similarity of word pairs (homonyms and homophones), while the seemingly humorous comments of the schizophrenic patient are rooted in an alteration of the semantic field of the word(s) employed. In spite of certain similarities in superficial structure there is a basic and specific difference in the semantic structure of these two forms of word play.", "contents": "[Word play and schizophrenic language disorder - what constitutes the difference?]. The listener often perceives a similarity between humorous comments by schizophrenic patients and linguistic word plays, especially puns. A structural analysis of specific examples illustrates the basic difference. In 'good' as well as 'bad' word plays the humorous effect stems from the surprising similarity of word pairs (homonyms and homophones), while the seemingly humorous comments of the schizophrenic patient are rooted in an alteration of the semantic field of the word(s) employed. In spite of certain similarities in superficial structure there is a basic and specific difference in the semantic structure of these two forms of word play."}
{"id": "PMID:477299", "title": "Relationship between one individual and another - relationship between the individual and society: limitations in the formation of psychotic disturbances.", "content": "The relations of the individual to others and family are understood through 'intersubjective' and/or 'interfamiliar' concepts. This 'inter' is always the origin of the relations between subjects; it is a reality which is of an atmospheric pneumatic nature. Its creative power and the uniqueness of the word are clarified by a selection of examples from oriental and occidental cultures. In contrast to it, the relations of the individual are also determined by norms, in which the 'objektive Geist' (Hegel) of a particular time is manifested. The individual is, in this relation, first of all a carrier of roles. A conditional analysis of psychotic disturbances must be based on the concept that the individual's psyche, in both spheres, is not a 'tabula rasa' (Locke), but always conveys a selection of that which has been offered in the situation (Leibniz). Out of this come constellations of a conditional nature conductive to the development of endogenous psychoses which exhibit themselves in the inter-sphere and role-sphere. These can only develop where a coherence of specific personality-structures with specific situations occurs. What makes various situations pathogenic for a particular, predisposed type, is a specific inference of meaning from peristatic precipitations.", "contents": "Relationship between one individual and another - relationship between the individual and society: limitations in the formation of psychotic disturbances. The relations of the individual to others and family are understood through 'intersubjective' and/or 'interfamiliar' concepts. This 'inter' is always the origin of the relations between subjects; it is a reality which is of an atmospheric pneumatic nature. Its creative power and the uniqueness of the word are clarified by a selection of examples from oriental and occidental cultures. In contrast to it, the relations of the individual are also determined by norms, in which the 'objektive Geist' (Hegel) of a particular time is manifested. The individual is, in this relation, first of all a carrier of roles. A conditional analysis of psychotic disturbances must be based on the concept that the individual's psyche, in both spheres, is not a 'tabula rasa' (Locke), but always conveys a selection of that which has been offered in the situation (Leibniz). Out of this come constellations of a conditional nature conductive to the development of endogenous psychoses which exhibit themselves in the inter-sphere and role-sphere. These can only develop where a coherence of specific personality-structures with specific situations occurs. What makes various situations pathogenic for a particular, predisposed type, is a specific inference of meaning from peristatic precipitations."}
{"id": "PMID:477312", "title": "Blood levels of medroxyprogesterone acetate after multiple injections of depoprovera or cycloprovera.", "content": "Mean levels of MPA in blood taken 90 days after injection were not significantly different between women who had received a single injection of DepoPrevera and those who had received eight injections at 90-day intervals. There was a wide variation in plasma MPA concentration in the two groups of women. In women who had received 31 to 45 injections of CycloProvera, plasma levels of MPA 28 days after injection were significantly higher than those of women who had received a single injection. The levels were also higher than those found in women 90 days after injection of DepoProvera. The results suggest that the dose of MPA in CycloProvera could be reduced.", "contents": "Blood levels of medroxyprogesterone acetate after multiple injections of depoprovera or cycloprovera. Mean levels of MPA in blood taken 90 days after injection were not significantly different between women who had received a single injection of DepoPrevera and those who had received eight injections at 90-day intervals. There was a wide variation in plasma MPA concentration in the two groups of women. In women who had received 31 to 45 injections of CycloProvera, plasma levels of MPA 28 days after injection were significantly higher than those of women who had received a single injection. The levels were also higher than those found in women 90 days after injection of DepoProvera. The results suggest that the dose of MPA in CycloProvera could be reduced."}
{"id": "PMID:477313", "title": "Bleeding and ovulation control with use of a small contraceptive vaginal ring releasing levonorgestrel and estradiol.", "content": "Levonorgestrel and estradiol releasing contraceptive vaginal rings (CVR) with an outer diameter of 50 mm were used by twenty women. The treatment was given in three-week cycles followed by one treatment-free week. The treatment was planned to cover six cycles. All subjects kept records of bleeding and were controlled clinically in the course of treatment. Three subjects were followed by blood sampling. Plasma levonorgestrel, estradiol, progesterone and gonadotropins were determined. The subjects experienced no difficulties in using the CVR and 90 per cent continued the treatment through the whole experimental period of six months. One subject discontinued after three cycles because of irregular bleedings and one subject after five cycles because of urinary discomfort. Regular bleedings were observed only in three subjects and in nine cases the bleeding started with the CVR in situ during the last days of the three-week treatment period. Breakthrough bleeding occurred in the remaining eight subjects. The subjective side-effects were as follows: weight gain in four subjects, oedema in one subject and urinary discomfort in one subject. Pituitary function was not generally suppressed as judged by plasma gonadotropins. Out of the three subjects studied, two experienced ovulatory plasma progesterone concentrations.", "contents": "Bleeding and ovulation control with use of a small contraceptive vaginal ring releasing levonorgestrel and estradiol. Levonorgestrel and estradiol releasing contraceptive vaginal rings (CVR) with an outer diameter of 50 mm were used by twenty women. The treatment was given in three-week cycles followed by one treatment-free week. The treatment was planned to cover six cycles. All subjects kept records of bleeding and were controlled clinically in the course of treatment. Three subjects were followed by blood sampling. Plasma levonorgestrel, estradiol, progesterone and gonadotropins were determined. The subjects experienced no difficulties in using the CVR and 90 per cent continued the treatment through the whole experimental period of six months. One subject discontinued after three cycles because of irregular bleedings and one subject after five cycles because of urinary discomfort. Regular bleedings were observed only in three subjects and in nine cases the bleeding started with the CVR in situ during the last days of the three-week treatment period. Breakthrough bleeding occurred in the remaining eight subjects. The subjective side-effects were as follows: weight gain in four subjects, oedema in one subject and urinary discomfort in one subject. Pituitary function was not generally suppressed as judged by plasma gonadotropins. Out of the three subjects studied, two experienced ovulatory plasma progesterone concentrations."}
{"id": "PMID:477314", "title": "Effect of prior pregnancy and combined oral contraceptives on baseline menstrual blood loss and bleeding response to intrauterine devices.", "content": "No effect of pregnancy was found on baseline menstrual blood loss (MBL) in women within one year of parturition or abortion. The increased bleeding response of women to intrauterine devices (IUDs) was found to be independent of pregnancy status during the year preceding IUD sertion. Women pregnant within a year of insertion had no different MBL than those pregnant more than a year prior to insertion. MBL quantified in subjects within three months of discontinuing combined oral contraceptives (OCs) was significantly lower than in prior non-OC users. Furthermore, MBL was significantly reduced in the former group during the first three menses following IUD insertion. At the sixth and twelfth menses post-insertion, MBL was still lower in prior OC users, but the difference between users and non-users was less and no longer statistically significant.", "contents": "Effect of prior pregnancy and combined oral contraceptives on baseline menstrual blood loss and bleeding response to intrauterine devices. No effect of pregnancy was found on baseline menstrual blood loss (MBL) in women within one year of parturition or abortion. The increased bleeding response of women to intrauterine devices (IUDs) was found to be independent of pregnancy status during the year preceding IUD sertion. Women pregnant within a year of insertion had no different MBL than those pregnant more than a year prior to insertion. MBL quantified in subjects within three months of discontinuing combined oral contraceptives (OCs) was significantly lower than in prior non-OC users. Furthermore, MBL was significantly reduced in the former group during the first three menses following IUD insertion. At the sixth and twelfth menses post-insertion, MBL was still lower in prior OC users, but the difference between users and non-users was less and no longer statistically significant."}
{"id": "PMID:477296", "title": "[Nonverbal communication and its implication for psychiatry].", "content": "Psychiatry unlike philosophy (with its problem of solipsism) recognizes the existence of other minds from the nonverbal communication between doctor and patient. This nonverbal, or having deeper roots in evolution, preverbal communication, is the connection within a herd or horde. As psychiatry gains many of its diagnostic criteria like mood, excitation or fear by nonverbal communication, it brings the patient in a biological-ethological frame where aim, biological sense and evolutional origin are justified questions of normal and abnormal behavior and of psychological events.", "contents": "[Nonverbal communication and its implication for psychiatry]. Psychiatry unlike philosophy (with its problem of solipsism) recognizes the existence of other minds from the nonverbal communication between doctor and patient. This nonverbal, or having deeper roots in evolution, preverbal communication, is the connection within a herd or horde. As psychiatry gains many of its diagnostic criteria like mood, excitation or fear by nonverbal communication, it brings the patient in a biological-ethological frame where aim, biological sense and evolutional origin are justified questions of normal and abnormal behavior and of psychological events."}
{"id": "PMID:477315", "title": "A randomized double blind study of two oral contraceptives.", "content": "To study the question of whether one brand of oral contraceptives may be as acceptable as another for use of publicly-assisted family planning programs, a double blind study of two well-known brands, Ovral and Norinyl, was undertaken in Costa Rica and Trinidad. The pills were randomly assigned to 1,200 women. Common side effects - nausea, dizziness, vomiting, headaches - were associated with both Norinyl and Ovral. Differences in event rates for these conditions were much more marked by country than by the pill used. Ovral was associated with increases in skin problems, notably chloasma, in Cost Rica. A higher percentage of women using Norinyl reported intermenstrual bleeding and spotting in both countries. In Costa Rica continuation rates for Norinyl were adversely affected by this. With these exceptions there appear to be no important differences between the brands that would affect their use in family planning programs.", "contents": "A randomized double blind study of two oral contraceptives. To study the question of whether one brand of oral contraceptives may be as acceptable as another for use of publicly-assisted family planning programs, a double blind study of two well-known brands, Ovral and Norinyl, was undertaken in Costa Rica and Trinidad. The pills were randomly assigned to 1,200 women. Common side effects - nausea, dizziness, vomiting, headaches - were associated with both Norinyl and Ovral. Differences in event rates for these conditions were much more marked by country than by the pill used. Ovral was associated with increases in skin problems, notably chloasma, in Cost Rica. A higher percentage of women using Norinyl reported intermenstrual bleeding and spotting in both countries. In Costa Rica continuation rates for Norinyl were adversely affected by this. With these exceptions there appear to be no important differences between the brands that would affect their use in family planning programs."}
{"id": "PMID:477316", "title": "Antifertility efficacy of the plant Aristolochia indica linn on mouse.", "content": "Two compounds isolated from the alcoholic extract of the roots of Aristolochia indica Linn were tested on day 6 pregnant mice. One of the compounds, p-coumaric acid showed 100% interceptive activity at the single oral dose of 50 mg/kg of body weight. The antifertility efficacy of these two compounds is discussed.", "contents": "Antifertility efficacy of the plant Aristolochia indica linn on mouse. Two compounds isolated from the alcoholic extract of the roots of Aristolochia indica Linn were tested on day 6 pregnant mice. One of the compounds, p-coumaric acid showed 100% interceptive activity at the single oral dose of 50 mg/kg of body weight. The antifertility efficacy of these two compounds is discussed."}
{"id": "PMID:477317", "title": "Microsurgical tubal anastomosis in the rabbit following three types of sterilisation procedure.", "content": "The reversibility of Falope ring, Pomeroy and unipolar cautery methods of sterilisation was examined in 30 rabbits. Using microsurgical techniques, an overall pregnancy rate of 56.6% was attained. Reversibility was more readily achieved and resulted in a higher pregnancy rate after Falope ring than Pomeroy or cautery sterilisation. The pregnancy rate after each method of sterilisation seems to correlate with the results obtained for reversal of sterilisation in the human.", "contents": "Microsurgical tubal anastomosis in the rabbit following three types of sterilisation procedure. The reversibility of Falope ring, Pomeroy and unipolar cautery methods of sterilisation was examined in 30 rabbits. Using microsurgical techniques, an overall pregnancy rate of 56.6% was attained. Reversibility was more readily achieved and resulted in a higher pregnancy rate after Falope ring than Pomeroy or cautery sterilisation. The pregnancy rate after each method of sterilisation seems to correlate with the results obtained for reversal of sterilisation in the human."}
{"id": "PMID:477320", "title": "Veterinary medicine in New York State: overview of distribution patterns.", "content": "An understanding of the distributional patterns of Veterinary practitioners in New York State can assist in optimizing veterinary services and opportunities. Veterinarians have been arrayed by type of practice and location within counties and major land form regions. Relationships between the number of practitioners and human and animal populations have been examined in these areas and show the inadequacy of basing veterinary needs on human population data. In contrast, large animal numbers show a much closer correspondence to veterinary practitioners, with ranges of 3341 to 6561 animals being serviced per veterinarian in the large and mixed service fields (98% of these totals are food animals). Data on age composition and degree institution (in-state versus out-of-state) provide indications of the length of service, turnover rate and retention rate for practitioners trained in New York State. At present 60 percent of New York State practitioners received their education at Cornell and more than three quarters of the active total completed their education since 1950.", "contents": "Veterinary medicine in New York State: overview of distribution patterns. An understanding of the distributional patterns of Veterinary practitioners in New York State can assist in optimizing veterinary services and opportunities. Veterinarians have been arrayed by type of practice and location within counties and major land form regions. Relationships between the number of practitioners and human and animal populations have been examined in these areas and show the inadequacy of basing veterinary needs on human population data. In contrast, large animal numbers show a much closer correspondence to veterinary practitioners, with ranges of 3341 to 6561 animals being serviced per veterinarian in the large and mixed service fields (98% of these totals are food animals). Data on age composition and degree institution (in-state versus out-of-state) provide indications of the length of service, turnover rate and retention rate for practitioners trained in New York State. At present 60 percent of New York State practitioners received their education at Cornell and more than three quarters of the active total completed their education since 1950."}
{"id": "PMID:477319", "title": "Pharmacokinetics of norethindrone in Indian Women.", "content": "Pharmacokinetics of norethindrone (NET) was evaluated in eleven women belonging to a low socio-economic group and in five womem belonging to the high socio-economic group after the administration of an oral dose of 0.35 mg NET minipill on an empty stomach. Blood samples were collected at different intervals of time over a period of 24 hours. Plasma NET was estimated by radioimmunoassay. In all women, peak levels of NET occurred within 1-2 hours and a semi-log plot of plasma NET levels showed a biexponential decline. The half-life of plasma NET clearance was relatively shorter in women of low socio-economic group with poor nutritional status as indicated by anthropometric indices, as compared to that in well nourished women of high socio-economic group. There was a significant positive correlation between weight/(height) 2x 100 index on the one hand and t1/2 (beta) on the other in all the women studied, thereby suggesting a role for nutritional status in the metabolic handling of NET.", "contents": "Pharmacokinetics of norethindrone in Indian Women. Pharmacokinetics of norethindrone (NET) was evaluated in eleven women belonging to a low socio-economic group and in five womem belonging to the high socio-economic group after the administration of an oral dose of 0.35 mg NET minipill on an empty stomach. Blood samples were collected at different intervals of time over a period of 24 hours. Plasma NET was estimated by radioimmunoassay. In all women, peak levels of NET occurred within 1-2 hours and a semi-log plot of plasma NET levels showed a biexponential decline. The half-life of plasma NET clearance was relatively shorter in women of low socio-economic group with poor nutritional status as indicated by anthropometric indices, as compared to that in well nourished women of high socio-economic group. There was a significant positive correlation between weight/(height) 2x 100 index on the one hand and t1/2 (beta) on the other in all the women studied, thereby suggesting a role for nutritional status in the metabolic handling of NET."}
{"id": "PMID:477323", "title": "Acquired hyposegmentation of granulocytes (pseudo-Pelger-Hu\u00ebt anomaly) in a dog.", "content": "Hyposegmentation of granulocyte nuclei was diagnosed in an adult dog after unsuccessful attempt at finding an occult chronic infection. Intermittent signs of prostatitis were temporarily observed, but the immature nature of circulating granulocytes persisted nine months. Electron microscopic examination and neutrophil function studies revealed no abnormalities. Since previous blood smear evaluation had been normal, it is proposed that the granulocyte anomaly was acquired and may have resulted from an idiosyncratic reaction to one of several chemotherapeutic agents administered.", "contents": "Acquired hyposegmentation of granulocytes (pseudo-Pelger-Hu\u00ebt anomaly) in a dog. Hyposegmentation of granulocyte nuclei was diagnosed in an adult dog after unsuccessful attempt at finding an occult chronic infection. Intermittent signs of prostatitis were temporarily observed, but the immature nature of circulating granulocytes persisted nine months. Electron microscopic examination and neutrophil function studies revealed no abnormalities. Since previous blood smear evaluation had been normal, it is proposed that the granulocyte anomaly was acquired and may have resulted from an idiosyncratic reaction to one of several chemotherapeutic agents administered."}
{"id": "PMID:477322", "title": "Nodular skin disease associated with Dirofilaria immitis infection in the dog.", "content": "Three dogs with dirofilariasis had a dermatologic syndrome, characterized by pruritus and multifocal nodules which tended to ulcerate. The cutaneous nodules revealed angiocentric pyogranulomatous inflammation with variable numbers of eosinophils. Microfilariae were often present in the blood vessels surrounded by the pyogranulomatous process. Therapy for dirofilariasis resulted in complete resolution of the dermatitis.", "contents": "Nodular skin disease associated with Dirofilaria immitis infection in the dog. Three dogs with dirofilariasis had a dermatologic syndrome, characterized by pruritus and multifocal nodules which tended to ulcerate. The cutaneous nodules revealed angiocentric pyogranulomatous inflammation with variable numbers of eosinophils. Microfilariae were often present in the blood vessels surrounded by the pyogranulomatous process. Therapy for dirofilariasis resulted in complete resolution of the dermatitis."}
{"id": "PMID:477325", "title": "Demodex cati Hirst 1919: a redescription.", "content": "All life stages of Demodex cati are described and compared with D. canis. Presence of D. cati is reported for the first time from the external auditory meatus. In the two cases examined mites occurred in large numbers with little pathogenic effect.", "contents": "Demodex cati Hirst 1919: a redescription. All life stages of Demodex cati are described and compared with D. canis. Presence of D. cati is reported for the first time from the external auditory meatus. In the two cases examined mites occurred in large numbers with little pathogenic effect."}
{"id": "PMID:477324", "title": "Ethylene glycol toxicosis in cattle.", "content": "A 1-month-old Jersey calf died of oxalate nephropathy. The calf had access to antifreeze (ethylene glycol) 3 days prior to death. Since ethylene glycol toxicosis had not been reported in cattle, the effects or oral administration of ethylene glycol were studied in 7 calves and 3 cows. The toxic dose ranged from 2 to 10 ml of ethylene glycol per kg of body weight. Clinical signs were increased respiration, staggering gait, paraparesis, depression and later, recumbency and death. Hemoglobinuria and epistaxis were seen at doses of 10mg/kg of body weight. Azotemia, hypocalcemia and neutrophilia were constant findings whereas acidosis, plasma hyperosmolality and hemolytic anemia were seen in the animals receiving the higher doses. A diagnosis of ethylene glycol toxicosis must be based upon a history of ingestion and the presence of calcium oxalate crystals in body tissues (especially the kidney and brain).", "contents": "Ethylene glycol toxicosis in cattle. A 1-month-old Jersey calf died of oxalate nephropathy. The calf had access to antifreeze (ethylene glycol) 3 days prior to death. Since ethylene glycol toxicosis had not been reported in cattle, the effects or oral administration of ethylene glycol were studied in 7 calves and 3 cows. The toxic dose ranged from 2 to 10 ml of ethylene glycol per kg of body weight. Clinical signs were increased respiration, staggering gait, paraparesis, depression and later, recumbency and death. Hemoglobinuria and epistaxis were seen at doses of 10mg/kg of body weight. Azotemia, hypocalcemia and neutrophilia were constant findings whereas acidosis, plasma hyperosmolality and hemolytic anemia were seen in the animals receiving the higher doses. A diagnosis of ethylene glycol toxicosis must be based upon a history of ingestion and the presence of calcium oxalate crystals in body tissues (especially the kidney and brain)."}
{"id": "PMID:477326", "title": "Surgical procedure for exposure of the chorda tympani in dogs: a ventral approach.", "content": "An experimental neuroanatomic investigation of the innervation of the dog's tongue by the method of retrograde axonal transport of horseradish peroxidase required that chorda tympani neurectomy be performed in neonatal pups. Surgical exposure and transection of the chorda tympani proximal to its union with the lingual branch of the mandibular nerve in the past has been accomplished by radical lateral approach which is suited only for acute experimentation. This report describes a ventral surgical exposure of the extracranial portion of the chorda tympani in the dog. This surgical procedure permits long-term survival and is considered especially useful for the chronic neuroanatomic and neurophysiologic investigations on the role of the chorda tympany in taste, salivation and lingual vasodilation in the dog.", "contents": "Surgical procedure for exposure of the chorda tympani in dogs: a ventral approach. An experimental neuroanatomic investigation of the innervation of the dog's tongue by the method of retrograde axonal transport of horseradish peroxidase required that chorda tympani neurectomy be performed in neonatal pups. Surgical exposure and transection of the chorda tympani proximal to its union with the lingual branch of the mandibular nerve in the past has been accomplished by radical lateral approach which is suited only for acute experimentation. This report describes a ventral surgical exposure of the extracranial portion of the chorda tympani in the dog. This surgical procedure permits long-term survival and is considered especially useful for the chronic neuroanatomic and neurophysiologic investigations on the role of the chorda tympany in taste, salivation and lingual vasodilation in the dog."}
{"id": "PMID:477321", "title": "An immunohematological study of chronic copper toxicity in sheep.", "content": "Direct and indirect Coombs tests were performed on six experimentally chronic copper poisoned sheep and on two control sheep. Initially, all sheep were direct Coombs test negative and five of six experimental and one of two control sheep were indirect Coombs test positive. During intoxication, five of six experimental sheep became direct Coombs test positive while controls remained negative. Eluates, prepared from the red cells of three experimental sheep, sensitized normal sheep red cells, as measured by the indirect Coombs test. Similarly prepared eluates from control sheep were negative.", "contents": "An immunohematological study of chronic copper toxicity in sheep. Direct and indirect Coombs tests were performed on six experimentally chronic copper poisoned sheep and on two control sheep. Initially, all sheep were direct Coombs test negative and five of six experimental and one of two control sheep were indirect Coombs test positive. During intoxication, five of six experimental sheep became direct Coombs test positive while controls remained negative. Eluates, prepared from the red cells of three experimental sheep, sensitized normal sheep red cells, as measured by the indirect Coombs test. Similarly prepared eluates from control sheep were negative."}
{"id": "PMID:477328", "title": "Relationship between clinical presentation of atrioventricular block and its localization defined by His bundle electrogram.", "content": "The frequency of clinical and electrocardiographic symptoms in dependence on the localization of the atrioventricular (AV) block, defined with the aid of His bundle electrogram, was investigated in 49 patients with 2nd-or 3rd-degree AV block. The Adams-Stokes syndrome, broad ventricular complex, and Mobitz II-type AV block were most frequently encountered in patients with infrahisian block, whereas Mobitz I type block was most frequently found in patients with suprahisian block. A chronic course of AV block was most frequent in patients with intrahisian block. The mean ventricular frequency of complete blockade was highest in suprahisian block, and lowest in infrahisian block. The localization of the block is a decisive factor for estimation of the prognosis and for indication of pacemaker implantation.", "contents": "Relationship between clinical presentation of atrioventricular block and its localization defined by His bundle electrogram. The frequency of clinical and electrocardiographic symptoms in dependence on the localization of the atrioventricular (AV) block, defined with the aid of His bundle electrogram, was investigated in 49 patients with 2nd-or 3rd-degree AV block. The Adams-Stokes syndrome, broad ventricular complex, and Mobitz II-type AV block were most frequently encountered in patients with infrahisian block, whereas Mobitz I type block was most frequently found in patients with suprahisian block. A chronic course of AV block was most frequent in patients with intrahisian block. The mean ventricular frequency of complete blockade was highest in suprahisian block, and lowest in infrahisian block. The localization of the block is a decisive factor for estimation of the prognosis and for indication of pacemaker implantation."}
{"id": "PMID:477329", "title": "Evaluation of the left ventricular ejection function by means of single beam echocardiography.", "content": "Echocardiographic examination of the left ventricle was performed in a group of 50 patients with various heart diseases. Five methods proposed for left ventricular pump function calculation were used, based on measurements of ventricular end-diastolic diameters. The results have shown that all tested methods of determining the ejection fraction or its functional equivalent (fractional shortening) correlate significantly with the ejection fraction obtained from the considered angiocardiographic technique. The closest correlation (r=0.771) was found when the ejection fraction was calculated from volume values determined by the cubed formula. Among other methods, that of fractional shortening and ejection fraction derived from those values seemed to be well interpretable. Other methods of ejection fraction calculation have a rationale in some cases, but have also their specific limitations.", "contents": "Evaluation of the left ventricular ejection function by means of single beam echocardiography. Echocardiographic examination of the left ventricle was performed in a group of 50 patients with various heart diseases. Five methods proposed for left ventricular pump function calculation were used, based on measurements of ventricular end-diastolic diameters. The results have shown that all tested methods of determining the ejection fraction or its functional equivalent (fractional shortening) correlate significantly with the ejection fraction obtained from the considered angiocardiographic technique. The closest correlation (r=0.771) was found when the ejection fraction was calculated from volume values determined by the cubed formula. Among other methods, that of fractional shortening and ejection fraction derived from those values seemed to be well interpretable. Other methods of ejection fraction calculation have a rationale in some cases, but have also their specific limitations."}
{"id": "PMID:477327", "title": "Accumulated creatine phosphokinase release as a prognostic index in the course of acute myocardial infarction.", "content": "Accumulated creatine phosphokinase (ACPK) in 25 patients with acute transmural myocardial infarction (MI) was evaluated as a prognostic criterion of clinical severity of the disease. It has been shown that ACPK values above 2.5U/ml are observed in 91.7% in MI complicated by pump failure. This does not apply to repeated MI. The possibility of mathematical estimation of the course of CPK release was also verified. The contribution of this method for the clinician is discussed.", "contents": "Accumulated creatine phosphokinase release as a prognostic index in the course of acute myocardial infarction. Accumulated creatine phosphokinase (ACPK) in 25 patients with acute transmural myocardial infarction (MI) was evaluated as a prognostic criterion of clinical severity of the disease. It has been shown that ACPK values above 2.5U/ml are observed in 91.7% in MI complicated by pump failure. This does not apply to repeated MI. The possibility of mathematical estimation of the course of CPK release was also verified. The contribution of this method for the clinician is discussed."}
{"id": "PMID:477331", "title": "Acute myocardial infarction--prehospital and posthospital phases.", "content": "The prognosis of acute myocardial infarction depends to a large extent on quick access to medical facilities, preferably in a hospital. Total delay in hospitalization (THD) consists of patient's delay (PD--time from the onset of the attack to the call for a doctor), doctor's delay (DD--time from the call for a doctor to his arrival), and hospitalization delay (HD--time from the doctor's arrival to the admission in a hospital). In a group of 507 cases of acute myocardial infarction the median THD was 215 min., PD--95 min., DD--20 min., HD--35 min. Better interpretation of symptoms both by the patient and the doctor seems to be one of the most effective means of shortening the total hospitalization delay. The final outcome of infarction in terms of the patient's return to normal life depends on proper rehabilitation and treatment in the posthospital phase.", "contents": "Acute myocardial infarction--prehospital and posthospital phases. The prognosis of acute myocardial infarction depends to a large extent on quick access to medical facilities, preferably in a hospital. Total delay in hospitalization (THD) consists of patient's delay (PD--time from the onset of the attack to the call for a doctor), doctor's delay (DD--time from the call for a doctor to his arrival), and hospitalization delay (HD--time from the doctor's arrival to the admission in a hospital). In a group of 507 cases of acute myocardial infarction the median THD was 215 min., PD--95 min., DD--20 min., HD--35 min. Better interpretation of symptoms both by the patient and the doctor seems to be one of the most effective means of shortening the total hospitalization delay. The final outcome of infarction in terms of the patient's return to normal life depends on proper rehabilitation and treatment in the posthospital phase."}
{"id": "PMID:477330", "title": "Capillary diffusion capacity for I-131 and capillary filtration rate in female patients with idiopathic oedema.", "content": "In female patients with idiopathic oedema the capillary filtration rate and capillary diffusion capacity for I-131 were studied in lower-limb muscles and subcutaneous tissue. The capillary filtration rate averaged 0.276 ml/100 ml per minute, and the capillary diffusion capacity for I-131 averaged 9.8 mol/(mol/ml) per 100 g per minute in the gastroonemius muscle and 8.7 mol/(mol/ml) per 100 g per minute in the lower-limb subcutaneous tissue. All of these figures are statistically significantly higher than the corresponding control findings. The results are compatible with the concept of disturbed capillary permeability, and etiopathogenetic factor of idiopathic oedema.", "contents": "Capillary diffusion capacity for I-131 and capillary filtration rate in female patients with idiopathic oedema. In female patients with idiopathic oedema the capillary filtration rate and capillary diffusion capacity for I-131 were studied in lower-limb muscles and subcutaneous tissue. The capillary filtration rate averaged 0.276 ml/100 ml per minute, and the capillary diffusion capacity for I-131 averaged 9.8 mol/(mol/ml) per 100 g per minute in the gastroonemius muscle and 8.7 mol/(mol/ml) per 100 g per minute in the lower-limb subcutaneous tissue. All of these figures are statistically significantly higher than the corresponding control findings. The results are compatible with the concept of disturbed capillary permeability, and etiopathogenetic factor of idiopathic oedema."}
{"id": "PMID:477332", "title": "Effects of centrally applied thiamenidine on pressure response to central nervous system stimulation.", "content": "Rise in blood pressure and heart rhythm disorders were provoked in rabbits submitted to electrical stimulation of the central nervous system. When injected into the cisterna magna, the hypotensive agent thiamenidine at a single dose of 100 microgram caused a fall in blood pressure and heart rate; this was not the case when the same dose was applied intravenously. Thiamenidine did not hinder the pressor response following stimulation of subthalamic zones, yet a reduction of rhythm disorders was obtained when the drug was administered by either route. The results suggest a central nervous action of thiamenidine.", "contents": "Effects of centrally applied thiamenidine on pressure response to central nervous system stimulation. Rise in blood pressure and heart rhythm disorders were provoked in rabbits submitted to electrical stimulation of the central nervous system. When injected into the cisterna magna, the hypotensive agent thiamenidine at a single dose of 100 microgram caused a fall in blood pressure and heart rate; this was not the case when the same dose was applied intravenously. Thiamenidine did not hinder the pressor response following stimulation of subthalamic zones, yet a reduction of rhythm disorders was obtained when the drug was administered by either route. The results suggest a central nervous action of thiamenidine."}
{"id": "PMID:477333", "title": "Estimation of size of infarcted focus by spatial quantitative vectorcardiography in patients with acute anterior myocardial infarction.", "content": "The study included 113 patients with acute myocardial infarction of the left ventricular anterior wall. The patients were repeatedly followed up from the first hours of the affection onward in the course of the individual stages of treatment (stage of intensive monitoring and therapy; intermediate stage; initial rehabilitation with application of active therapy; early mobilization, activation and rehabilitation). A new approach to the estimation of the size of the infarcted focus is described, namely, the method using spatial quantitative vectorcardiography. It was found that the spatial localization of the 40-ms vector, especially its azimuth, is a specific indicator, with the aid of which the localization, extent and depth of the lesion in the left ventricular anterior wall can be assessed with a high probability in most instances. The compensatory adaptive and reparative capacity of the affected myocardium, estimated on the basis of spatial quantitative VCG, is closely correlated with the size of the infarcted focus.", "contents": "Estimation of size of infarcted focus by spatial quantitative vectorcardiography in patients with acute anterior myocardial infarction. The study included 113 patients with acute myocardial infarction of the left ventricular anterior wall. The patients were repeatedly followed up from the first hours of the affection onward in the course of the individual stages of treatment (stage of intensive monitoring and therapy; intermediate stage; initial rehabilitation with application of active therapy; early mobilization, activation and rehabilitation). A new approach to the estimation of the size of the infarcted focus is described, namely, the method using spatial quantitative vectorcardiography. It was found that the spatial localization of the 40-ms vector, especially its azimuth, is a specific indicator, with the aid of which the localization, extent and depth of the lesion in the left ventricular anterior wall can be assessed with a high probability in most instances. The compensatory adaptive and reparative capacity of the affected myocardium, estimated on the basis of spatial quantitative VCG, is closely correlated with the size of the infarcted focus."}
{"id": "PMID:477335", "title": "Lateral asymmetry in testing long-term memory for faces.", "content": "Accuracy of recognition memory for faces initially seen in the central visual field was found to be greater 48 hours when previously seen and new faces were presented to the left visual field than when presentation was to for right visual field. It is argued that this result reflects relative specialization for the right hemisphere in storing visual patterns.", "contents": "Lateral asymmetry in testing long-term memory for faces. Accuracy of recognition memory for faces initially seen in the central visual field was found to be greater 48 hours when previously seen and new faces were presented to the left visual field than when presentation was to for right visual field. It is argued that this result reflects relative specialization for the right hemisphere in storing visual patterns."}
{"id": "PMID:477336", "title": "Incidental learning of image-mediated or pronounced words after right temporal lobectomy.", "content": "The use of visual images in word recall by patients with right temporal lobectomy was studied by testing the incidental learning of 40 (20 abstract, 20 concrete) words that had been either carefully pronounced or the referent visualized. When tested immediately after presentation of the list, the right temporal-lobe group (N = 30) did not differ from a matched normal control group, but in delayed recall 2 hours later the patient group showed impaired recall of concrete words that had been visualized. Recall of visualized abstract words was also poor but not significantly so, while pronounced words, whether abstract or concrete, were recalled normally. Further analysis revealed an impaired recall for visualized concrete words in both immediate and delayed testing in patients having a large right hippocampal removal, indicating an important role of the mesial temporal structures in image recall.", "contents": "Incidental learning of image-mediated or pronounced words after right temporal lobectomy. The use of visual images in word recall by patients with right temporal lobectomy was studied by testing the incidental learning of 40 (20 abstract, 20 concrete) words that had been either carefully pronounced or the referent visualized. When tested immediately after presentation of the list, the right temporal-lobe group (N = 30) did not differ from a matched normal control group, but in delayed recall 2 hours later the patient group showed impaired recall of concrete words that had been visualized. Recall of visualized abstract words was also poor but not significantly so, while pronounced words, whether abstract or concrete, were recalled normally. Further analysis revealed an impaired recall for visualized concrete words in both immediate and delayed testing in patients having a large right hippocampal removal, indicating an important role of the mesial temporal structures in image recall."}
{"id": "PMID:477337", "title": "Naming to picture versus description in three aphasic subgroups.", "content": "Twenty-three male aphasics, classified as Broca's, Wernicke's and anomic, were tested on their ability to name objects to either visual confrontation or oral associative description. For the Broca's and Wernicke's aphasics, naming to visual confrontation was significantly better than to oral description. Broca's aphasics tended to produce more correct names than both other groups in both conditions, although significance was reached only in oral description. Differences were not significantly affected by the comprehension deficit or severity of aphasia. With picture naming, all groups tended to name immediately or not al all. For oral description, there was a greater proportion of long latency responses for the posterior patients. When given phonemic or visual cues to assist naming, Broca's aphasics benefited the most, the anomics performing most poorly. All these results suggest qualitative differences in naming ability in aphasic subgroups.", "contents": "Naming to picture versus description in three aphasic subgroups. Twenty-three male aphasics, classified as Broca's, Wernicke's and anomic, were tested on their ability to name objects to either visual confrontation or oral associative description. For the Broca's and Wernicke's aphasics, naming to visual confrontation was significantly better than to oral description. Broca's aphasics tended to produce more correct names than both other groups in both conditions, although significance was reached only in oral description. Differences were not significantly affected by the comprehension deficit or severity of aphasia. With picture naming, all groups tended to name immediately or not al all. For oral description, there was a greater proportion of long latency responses for the posterior patients. When given phonemic or visual cues to assist naming, Broca's aphasics benefited the most, the anomics performing most poorly. All these results suggest qualitative differences in naming ability in aphasic subgroups."}
{"id": "PMID:477339", "title": "Cognitive impairment of aphasics in a colour-to-picture matching task.", "content": "Broca's and Wernicke's aphasics, non-aphasic brain-damaged patients and chronic schizophrenics were tested in a colour-to-picture matching task. For each of 42 black-and-white drawings of objects the most \"typical\" colour was to be selected from a set of 24 colour strips. Separate scores for the appropriateness of the choices as to hue, brightness, and saturation were obtained by comparison with the data from 50 normal Ss. The choices of both aphasic groups were more deviant than those of brain-damaged controls for chromatically coloured objects with respect to all three colour dimensions, and for achromatically coloured objects with respect to saturation and brightness. Wherever the aphasics' performance was worse than that of the controls, the deviancy-scores correlated significantly with the Token Test. Results are interpreted as an impairment of aphasics in the ability to isolate analytically specific features of objects or concepts which might be conceived of as a prerequisite of propositional language.", "contents": "Cognitive impairment of aphasics in a colour-to-picture matching task. Broca's and Wernicke's aphasics, non-aphasic brain-damaged patients and chronic schizophrenics were tested in a colour-to-picture matching task. For each of 42 black-and-white drawings of objects the most \"typical\" colour was to be selected from a set of 24 colour strips. Separate scores for the appropriateness of the choices as to hue, brightness, and saturation were obtained by comparison with the data from 50 normal Ss. The choices of both aphasic groups were more deviant than those of brain-damaged controls for chromatically coloured objects with respect to all three colour dimensions, and for achromatically coloured objects with respect to saturation and brightness. Wherever the aphasics' performance was worse than that of the controls, the deviancy-scores correlated significantly with the Token Test. Results are interpreted as an impairment of aphasics in the ability to isolate analytically specific features of objects or concepts which might be conceived of as a prerequisite of propositional language."}
{"id": "PMID:477338", "title": "Problems in the acquisition of imagery mnemonics: three brain-damaged cases.", "content": "The literature provides little direction on how to overcome difficulties which some brain-damaged patients have in acquiring imagery mnemonics as a memory aid during the period of anterograde amnesia. For those interested in the therepeutic usefulness of imagery mediation, we provide a detailed account of the acquisition of some mnemonic skill in three brain-damaged patients who initially failed in using visual imagery mediators to recall words lists.", "contents": "Problems in the acquisition of imagery mnemonics: three brain-damaged cases. The literature provides little direction on how to overcome difficulties which some brain-damaged patients have in acquiring imagery mnemonics as a memory aid during the period of anterograde amnesia. For those interested in the therepeutic usefulness of imagery mediation, we provide a detailed account of the acquisition of some mnemonic skill in three brain-damaged patients who initially failed in using visual imagery mediators to recall words lists."}
{"id": "PMID:477340", "title": "Semantic judgment ability in adult aphasia.", "content": "Recent psycholinguistic literature suggests that language usage often involves the ability to make judgments or appraisals about various aspects of language. It was therefore the purpose of the present study to examine the semantic judgment or evaluation abilities of thirty persons with aphasia in comparison to these same behaviors in a group of thirty normal individuals. That is, this study examined subject ability to use knowledge to make comparisons or judgments in reference to specific criteria. Results support the existence of a judgment capacity and indicate that persons with aphasia are impaired in their ability to evaluate semantic material. Therefore, speech pathologists may wish to include semantic judgment tasks in their evaluation procedures and plan therapy directed toward the retrieval of judgment responses.", "contents": "Semantic judgment ability in adult aphasia. Recent psycholinguistic literature suggests that language usage often involves the ability to make judgments or appraisals about various aspects of language. It was therefore the purpose of the present study to examine the semantic judgment or evaluation abilities of thirty persons with aphasia in comparison to these same behaviors in a group of thirty normal individuals. That is, this study examined subject ability to use knowledge to make comparisons or judgments in reference to specific criteria. Results support the existence of a judgment capacity and indicate that persons with aphasia are impaired in their ability to evaluate semantic material. Therefore, speech pathologists may wish to include semantic judgment tasks in their evaluation procedures and plan therapy directed toward the retrieval of judgment responses."}
{"id": "PMID:477341", "title": "Individual differences and asymmetry effects in memory for unfamiliar faces.", "content": "Visual field effects for recognition of unfamiliar faces was investigated using memory intervals of 0, 10, and 20 sec. and 8 stimuli in a same-different comparison paradigm. In Experiment I, 24 male and 24 female familial righthanders, half of whom had high and the other half, low Block Design (BD) scaled scores participated. Faces were recognized significantly more often in the LVF. A visual field x memory interval interaction resulted from reliable LVF superiorities with the 0 and 10 sec. memory intervals and a non-significant RVF superiority with the 20 sec. memory interval. Performance decreased significantly as the duration of the memory interval increased. No sex differences were found. Subjects with high and low BD scores performed similarly. In Experiment II, 16 stimuli were employed and the task repeated. Twenty-four male and 24 female familial righthanderds were given the BD and Vocabulary subtests of the WAIS as well as a brightness discrimination task. Field effects were non-significant. Performance decreased with longer memory intervals. For males, field effects for the 0 sec. memory interval were positively related to brightness discrimination scores.", "contents": "Individual differences and asymmetry effects in memory for unfamiliar faces. Visual field effects for recognition of unfamiliar faces was investigated using memory intervals of 0, 10, and 20 sec. and 8 stimuli in a same-different comparison paradigm. In Experiment I, 24 male and 24 female familial righthanders, half of whom had high and the other half, low Block Design (BD) scaled scores participated. Faces were recognized significantly more often in the LVF. A visual field x memory interval interaction resulted from reliable LVF superiorities with the 0 and 10 sec. memory intervals and a non-significant RVF superiority with the 20 sec. memory interval. Performance decreased significantly as the duration of the memory interval increased. No sex differences were found. Subjects with high and low BD scores performed similarly. In Experiment II, 16 stimuli were employed and the task repeated. Twenty-four male and 24 female familial righthanderds were given the BD and Vocabulary subtests of the WAIS as well as a brightness discrimination task. Field effects were non-significant. Performance decreased with longer memory intervals. For males, field effects for the 0 sec. memory interval were positively related to brightness discrimination scores."}
{"id": "PMID:477342", "title": "Impaired recognition memory after head injury.", "content": "A continuous recognition memory task employing 120 line drawings of familiar stimuli was administered to patients recovering from closed head injury of varying severity and control patients. Mildly injured patients obtained significantly more correct responses, and higher memory sensitivity (d') values than moderately and severely injured patients who performed similarly. The measure of response criterion, c, was significantly lower in patients with injury of moderate severity when compared to mildly injured cases and control patients. Controls and mildly injured patients did not differ in their performance. In general, the total correct responses most impressively differentiated head injured patients with measurable coma duration as more than two-thirds had scores below the control group. Group differences in hits, false alarms and misses were examined also. Age and education, skull fracture, and hematoma were generally unrelated to any of the measures, whereas coma duration was significantly related to most measures of mnemonic efficiency.", "contents": "Impaired recognition memory after head injury. A continuous recognition memory task employing 120 line drawings of familiar stimuli was administered to patients recovering from closed head injury of varying severity and control patients. Mildly injured patients obtained significantly more correct responses, and higher memory sensitivity (d') values than moderately and severely injured patients who performed similarly. The measure of response criterion, c, was significantly lower in patients with injury of moderate severity when compared to mildly injured cases and control patients. Controls and mildly injured patients did not differ in their performance. In general, the total correct responses most impressively differentiated head injured patients with measurable coma duration as more than two-thirds had scores below the control group. Group differences in hits, false alarms and misses were examined also. Age and education, skull fracture, and hematoma were generally unrelated to any of the measures, whereas coma duration was significantly related to most measures of mnemonic efficiency."}
{"id": "PMID:477343", "title": "Differential effects of age and cerebral atrophy upon span of immediate recall and paired-associate learning in older patients suspected of dementia.", "content": "Seventy-eight patients suspected of dementia, 50 years of age or older and without evidence of focal cerebral lesion or systemic illness, were administered the Digit-Span Forward and Paired-Associate Learning subtests of the Wechsler Memory Scale. Computerized axial tomography, allowing a visualization and measurement of the lateral ventricles and cortical sulci, was employed to measure cerebral atrophy. Four groups were defined as follows: (1) age 50-69, without atrophy (N = 27), (2) age 50-69, with atrophy (N = 17), (3) age 70-89, without atrophy (N = 18), (4) age 70-89, with atrophy (N = 16). Results of analyses of variance indicate that Digit-Span is affected by cerebral atrophy but not by age, while Paired-Associate Learning is affected by both age and cerebral atrophy. No interaction effect between age and cerebral atrophy was found for either task. These results are discussed within the context of previous research, and implications for both models of memory and theories of the effects of cerebral atrophy in different ag groups are suggested.", "contents": "Differential effects of age and cerebral atrophy upon span of immediate recall and paired-associate learning in older patients suspected of dementia. Seventy-eight patients suspected of dementia, 50 years of age or older and without evidence of focal cerebral lesion or systemic illness, were administered the Digit-Span Forward and Paired-Associate Learning subtests of the Wechsler Memory Scale. Computerized axial tomography, allowing a visualization and measurement of the lateral ventricles and cortical sulci, was employed to measure cerebral atrophy. Four groups were defined as follows: (1) age 50-69, without atrophy (N = 27), (2) age 50-69, with atrophy (N = 17), (3) age 70-89, without atrophy (N = 18), (4) age 70-89, with atrophy (N = 16). Results of analyses of variance indicate that Digit-Span is affected by cerebral atrophy but not by age, while Paired-Associate Learning is affected by both age and cerebral atrophy. No interaction effect between age and cerebral atrophy was found for either task. These results are discussed within the context of previous research, and implications for both models of memory and theories of the effects of cerebral atrophy in different ag groups are suggested."}
{"id": "PMID:477344", "title": "Recovery from alexia without agraphia: report of an autopsy.", "content": "The patient is a 58-year-old Japanese teacher of German literature who suffered twice from cerebrovascular accidents, showing alexia without agraphia. Pathological examination showed an old infarct in the posterior two-thirds of the fusiform and almost the whole lingual gyrus, involving the posterior border of the parahippocampal gyrus in the left hemisphere. The left cuneus and the calcarine cortex were preserved. There was degeneration of the lower third of the splenium of the corpus callosum, extending to its occipital radiation and tapetum on both sides. Comparing clinico-pathological findings of the 31 known autopsy cases, it was proposed that the lesion of the left spleno-lingual system produces alexia without agraphia but it may ameliorate. In addition, when spleno-cuneate system is also involved alexia becomes persistent and it may accompany object agnosia or optic aphasia.", "contents": "Recovery from alexia without agraphia: report of an autopsy. The patient is a 58-year-old Japanese teacher of German literature who suffered twice from cerebrovascular accidents, showing alexia without agraphia. Pathological examination showed an old infarct in the posterior two-thirds of the fusiform and almost the whole lingual gyrus, involving the posterior border of the parahippocampal gyrus in the left hemisphere. The left cuneus and the calcarine cortex were preserved. There was degeneration of the lower third of the splenium of the corpus callosum, extending to its occipital radiation and tapetum on both sides. Comparing clinico-pathological findings of the 31 known autopsy cases, it was proposed that the lesion of the left spleno-lingual system produces alexia without agraphia but it may ameliorate. In addition, when spleno-cuneate system is also involved alexia becomes persistent and it may accompany object agnosia or optic aphasia."}
{"id": "PMID:477345", "title": "Ipsilateral and contralateral reactions to monaural lateralized stimuli.", "content": "Twelve subjects, six right-handers and six left-handers, participated in an experiment in which they were tested on an ipsilateral and contralateral auditory-manual reaction-time task. No difference in reaction-time was found between left and right ear presentation in the ipsilateral task, whereas for nearly all subjects, right-handers as well as left-handers, a difference between the left and right ear presentation was recorded in the contralateral task. The right-handers showed a tendency to a right ear preference, the left-handers to a left ear preference. The results are discussed in terms of a combination of S-R compatibility and the cerebral organization in man.", "contents": "Ipsilateral and contralateral reactions to monaural lateralized stimuli. Twelve subjects, six right-handers and six left-handers, participated in an experiment in which they were tested on an ipsilateral and contralateral auditory-manual reaction-time task. No difference in reaction-time was found between left and right ear presentation in the ipsilateral task, whereas for nearly all subjects, right-handers as well as left-handers, a difference between the left and right ear presentation was recorded in the contralateral task. The right-handers showed a tendency to a right ear preference, the left-handers to a left ear preference. The results are discussed in terms of a combination of S-R compatibility and the cerebral organization in man."}
{"id": "PMID:477347", "title": "Handedness and sleep duration.", "content": "The differences in habitual sleep duration and absolute deviations in habitual sleep duration from the normative mean between 60 left-, 36 mixed- and 125 right-handed college students were tested. It was found that mixed-handers tend to sleep less and as a group show significantly greater deviations in habitual sleep durations than either left- or right-handers. The heuristic value of this relationship for future research was discussed briefly.", "contents": "Handedness and sleep duration. The differences in habitual sleep duration and absolute deviations in habitual sleep duration from the normative mean between 60 left-, 36 mixed- and 125 right-handed college students were tested. It was found that mixed-handers tend to sleep less and as a group show significantly greater deviations in habitual sleep durations than either left- or right-handers. The heuristic value of this relationship for future research was discussed briefly."}
{"id": "PMID:477346", "title": "Alpha hemispheric asymmetry of males and females on verbal and non-verbal tasks: some preliminary results.", "content": "The present study gathered preliminary data from males and females for verbal and non-verbal tasks to explore differences in alpha hemispheric asymmetry. Results indicated a significant task by hemispheric interaction effect which revealed significantly less alpha for the left hemisphere, as compared to the right hemisphere, for the verbal task. Significantly less alpha was found for the right hemisphere during the non-verbal task as compared to the right hemisphere on the verbal task. While significant differences between males and females were not revealed, task, subject, and stimuli differences are discussed as variables which may contribute to results obtained from hemispheric processing research.", "contents": "Alpha hemispheric asymmetry of males and females on verbal and non-verbal tasks: some preliminary results. The present study gathered preliminary data from males and females for verbal and non-verbal tasks to explore differences in alpha hemispheric asymmetry. Results indicated a significant task by hemispheric interaction effect which revealed significantly less alpha for the left hemisphere, as compared to the right hemisphere, for the verbal task. Significantly less alpha was found for the right hemisphere during the non-verbal task as compared to the right hemisphere on the verbal task. While significant differences between males and females were not revealed, task, subject, and stimuli differences are discussed as variables which may contribute to results obtained from hemispheric processing research."}
{"id": "PMID:477348", "title": "Early childhood amnesia: a quantitative study with implications for the study of retrograde amnesia after brain injury.", "content": "Measurement of retrieval of episodic memories ascribed to the various years of early childhood was made over a three-month period of search by 17 normal young adults. The distribution of episodes ascribed to various ages is described, and it is shown that the recovery of memory from early childhood tends to show features of shrinking retrograde amnesia. Methods which were used to recover very early episodes are described, and implications for the study of shrinking retrograde amnesia in brain-damaged patients are discussed.", "contents": "Early childhood amnesia: a quantitative study with implications for the study of retrograde amnesia after brain injury. Measurement of retrieval of episodic memories ascribed to the various years of early childhood was made over a three-month period of search by 17 normal young adults. The distribution of episodes ascribed to various ages is described, and it is shown that the recovery of memory from early childhood tends to show features of shrinking retrograde amnesia. Methods which were used to recover very early episodes are described, and implications for the study of shrinking retrograde amnesia in brain-damaged patients are discussed."}
{"id": "PMID:477349", "title": "Evaluation of delayed auditory feedback (DAF) effect: comparison between subjective judgments and objective measures.", "content": "The effect of Delayed Auditory Feedback (DAF) on speech duration was evaluated subjectively and measured objectively in 30 subjects (10 normals, 10 nonfluent aphasics and 10 fluent aphasia). The average correlation coefficient between the subjective and objective data was .52 for 180 msec. delay and .46 for 360 msec. delay. Analysis of variance of data derived from the two methods showed the same highly significant differences among groups, delay and tasks. We conclude from these results that both objective and subjective procedures are equally useful in evaluating DAF effect on speech duration, and that both may also be valuable in assessing other types of behavior.", "contents": "Evaluation of delayed auditory feedback (DAF) effect: comparison between subjective judgments and objective measures. The effect of Delayed Auditory Feedback (DAF) on speech duration was evaluated subjectively and measured objectively in 30 subjects (10 normals, 10 nonfluent aphasics and 10 fluent aphasia). The average correlation coefficient between the subjective and objective data was .52 for 180 msec. delay and .46 for 360 msec. delay. Analysis of variance of data derived from the two methods showed the same highly significant differences among groups, delay and tasks. We conclude from these results that both objective and subjective procedures are equally useful in evaluating DAF effect on speech duration, and that both may also be valuable in assessing other types of behavior."}
{"id": "PMID:477353", "title": "Significance and application of blood gas determinations from the left atrial catheter.", "content": "Left atrial catheters are used to measure left heart filling pressure in patients after open-heart surgery. It was observed that in some patients blood gases obtained from the left atrial catheters had a markedly higher PO2 as compared to PaO2 in the presence of severe hypoxemia. Twenty-five patients were studied consecutively; pulmonary venous admixture calculated from arterial blood was higher in 19 patients and lower in 5 as compared with that calculated from blood withdrawn from the left atrial catheter. These differences in venous admixture are due to regional changes in the lungs. This observation can be utilized in concentrating respiratory therapy to the regions of the lungs involved with significant therapeutic benefit to the patient.", "contents": "Significance and application of blood gas determinations from the left atrial catheter. Left atrial catheters are used to measure left heart filling pressure in patients after open-heart surgery. It was observed that in some patients blood gases obtained from the left atrial catheters had a markedly higher PO2 as compared to PaO2 in the presence of severe hypoxemia. Twenty-five patients were studied consecutively; pulmonary venous admixture calculated from arterial blood was higher in 19 patients and lower in 5 as compared with that calculated from blood withdrawn from the left atrial catheter. These differences in venous admixture are due to regional changes in the lungs. This observation can be utilized in concentrating respiratory therapy to the regions of the lungs involved with significant therapeutic benefit to the patient."}
{"id": "PMID:477352", "title": "Effects of closed-chest cardiac massage on intracranial pressure.", "content": "Closed-chest cardiac massage results in a marked increase in intrathoracic pressure and unusual patterns of blood flow. Among the physiological consequences of these changes appears to be a marked increase in intracranial pressure associated with chest compression as documented by the following patient case studies. While temporary, the marked nature of this rise in intracranial pressure suggests that the technique of closed-chest massage may, in itself, be responsible for clinically significant alterations in cerebral hemodynamics.", "contents": "Effects of closed-chest cardiac massage on intracranial pressure. Closed-chest cardiac massage results in a marked increase in intrathoracic pressure and unusual patterns of blood flow. Among the physiological consequences of these changes appears to be a marked increase in intracranial pressure associated with chest compression as documented by the following patient case studies. While temporary, the marked nature of this rise in intracranial pressure suggests that the technique of closed-chest massage may, in itself, be responsible for clinically significant alterations in cerebral hemodynamics."}
{"id": "PMID:477354", "title": "Oxygen consumption and recovery from surgical shock in rats: a comparison of the efficacy of different plasma substitutes.", "content": "Shock was induced in 537 rats by exteriorization of the intestines and occlusion of the superior mesenteric circulation for 1 hour. After 1 hour of this intestinal ischemia shock, oxygen consumption (VO2) decreased to half of the preexperimental values. When no infusion was given, the survival rate at 24 hours was 22%; this was correlated with the degree of restoration of VO2 at 1 hour after shock. VO2 and survival rate improved with infusion of albumin, dextran 40, and dextran 40, and dextran 70. With increasing doses of colloids, both VO2 and survival rate increased; the optimal effect was at the dose of 2 g/kg body weight. When no other therapy was given, colloids at concentrations of 3.5 or 6% solutions had a better effect on survival than the 10% solution. Colloids were more effective than Ringer's acetate when the latter was given in the same volume and up to three times the volume of the colloids.", "contents": "Oxygen consumption and recovery from surgical shock in rats: a comparison of the efficacy of different plasma substitutes. Shock was induced in 537 rats by exteriorization of the intestines and occlusion of the superior mesenteric circulation for 1 hour. After 1 hour of this intestinal ischemia shock, oxygen consumption (VO2) decreased to half of the preexperimental values. When no infusion was given, the survival rate at 24 hours was 22%; this was correlated with the degree of restoration of VO2 at 1 hour after shock. VO2 and survival rate improved with infusion of albumin, dextran 40, and dextran 40, and dextran 70. With increasing doses of colloids, both VO2 and survival rate increased; the optimal effect was at the dose of 2 g/kg body weight. When no other therapy was given, colloids at concentrations of 3.5 or 6% solutions had a better effect on survival than the 10% solution. Colloids were more effective than Ringer's acetate when the latter was given in the same volume and up to three times the volume of the colloids."}
{"id": "PMID:477377", "title": "Cryosurgery of verrucous carcinoma of the penis (Buschke-Lowenstein tumor).", "content": "Verrucous carcinoma of the penis (Buschke-Lowenstein tumor, giant condyloma accuminatum) is a common clearly defined variant of epidermoid carcinoma which features relentless local growth and little tendency to metastasize. Two cases successfully treated by liquid nitrogen cryosurgery are presented herein.", "contents": "Cryosurgery of verrucous carcinoma of the penis (Buschke-Lowenstein tumor). Verrucous carcinoma of the penis (Buschke-Lowenstein tumor, giant condyloma accuminatum) is a common clearly defined variant of epidermoid carcinoma which features relentless local growth and little tendency to metastasize. Two cases successfully treated by liquid nitrogen cryosurgery are presented herein."}
{"id": "PMID:477376", "title": "Oculoauriculovertebral dysplasia (Goldenhar's syndrome).", "content": "Oculoauriculovertebral dysplasia is a developmental disorder characterized by a triad of anomalies; epibulbar dermoids with or without lipodermoids, preauricular appendages or blind preauricular fistulae, and vertebral anomalies. Multiple associated anomalies have been reported. A case of this syndrome and a review of the literature are presented herein.", "contents": "Oculoauriculovertebral dysplasia (Goldenhar's syndrome). Oculoauriculovertebral dysplasia is a developmental disorder characterized by a triad of anomalies; epibulbar dermoids with or without lipodermoids, preauricular appendages or blind preauricular fistulae, and vertebral anomalies. Multiple associated anomalies have been reported. A case of this syndrome and a review of the literature are presented herein."}
{"id": "PMID:477378", "title": "Acquired digital fibrokeratoma.", "content": "In 1965, Steele reported three cases of garlic-clove fibromas that histologically resembled the acquired digital fibrokeratoma described by Bart in 1968. We present a case that clinically resembles garlic-clove fibroma and fits the histologic criteria of acquired digital fibrokeratoma. We believe they are the same entity.", "contents": "Acquired digital fibrokeratoma. In 1965, Steele reported three cases of garlic-clove fibromas that histologically resembled the acquired digital fibrokeratoma described by Bart in 1968. We present a case that clinically resembles garlic-clove fibroma and fits the histologic criteria of acquired digital fibrokeratoma. We believe they are the same entity."}
{"id": "PMID:477380", "title": "Arteriovenous hemangioma.", "content": "A case of the recently described vascular tumor, the arteriovenous hemangioma, is presented herein. Clinical and histologic differentiation from other benign, acquired vascular lesions is stressed.", "contents": "Arteriovenous hemangioma. A case of the recently described vascular tumor, the arteriovenous hemangioma, is presented herein. Clinical and histologic differentiation from other benign, acquired vascular lesions is stressed."}
{"id": "PMID:477381", "title": "Propranolol and alopecia.", "content": "A case is presented herein of alopecia developing in a patient receiving propranolol. The literature is reviewed and this case compared to the first one reported. The two cases have similarities, and most importantly, both showed an effluvium of the telogen type. This may be important as a diagnostic aid, if substantiated in further cases. In a survey of several major textbooks this side effect of propranolol is not noted and it is hoped that this article will make practitioners aware of the possible complication.", "contents": "Propranolol and alopecia. A case is presented herein of alopecia developing in a patient receiving propranolol. The literature is reviewed and this case compared to the first one reported. The two cases have similarities, and most importantly, both showed an effluvium of the telogen type. This may be important as a diagnostic aid, if substantiated in further cases. In a survey of several major textbooks this side effect of propranolol is not noted and it is hoped that this article will make practitioners aware of the possible complication."}
{"id": "PMID:477379", "title": "Sarcoid granulomas in venipuncture sites.", "content": "Scar changes may be an important clue in the diagnosis of sarcoidosis. Any patient complaining of changes in scars, areas of trauma, or venipuncture sites, should be evaluated for sarcoidosis. In patients already suspected of having the disease, these areas should not be overlooked as an easy source of tissue specimens for histopathologic confirmation of the diagnosis.", "contents": "Sarcoid granulomas in venipuncture sites. Scar changes may be an important clue in the diagnosis of sarcoidosis. Any patient complaining of changes in scars, areas of trauma, or venipuncture sites, should be evaluated for sarcoidosis. In patients already suspected of having the disease, these areas should not be overlooked as an easy source of tissue specimens for histopathologic confirmation of the diagnosis."}
{"id": "PMID:477382", "title": "Annular atrophic plaques of the face and upper body. An unusual variant of lichen sclerosus et atrophicus or lichen planus.", "content": "A particular scarring atrophic disorder occurring in tropical countries and usually affecting dark-skinned persons is presented herein.", "contents": "Annular atrophic plaques of the face and upper body. An unusual variant of lichen sclerosus et atrophicus or lichen planus. A particular scarring atrophic disorder occurring in tropical countries and usually affecting dark-skinned persons is presented herein."}
{"id": "PMID:477386", "title": "Localized tick bite reaction.", "content": "A woman presented with a localized tick bite reaction. The tick (Amblyomma americanum, the \"lone star\" tick) was still attached to the erythematous, indurated skin of the upper back. After several unsuccessful attempts at removal, a punch biopsy was performed, and the resultant histologic sections incorporated both tick and involved skin. The various systemic and localized tick bite reactions are discussed, as are the diagnostic dilemmas which may arise when the organism is no longer present. Punch biopsy is suggested as an effective means of removing the organism.", "contents": "Localized tick bite reaction. A woman presented with a localized tick bite reaction. The tick (Amblyomma americanum, the \"lone star\" tick) was still attached to the erythematous, indurated skin of the upper back. After several unsuccessful attempts at removal, a punch biopsy was performed, and the resultant histologic sections incorporated both tick and involved skin. The various systemic and localized tick bite reactions are discussed, as are the diagnostic dilemmas which may arise when the organism is no longer present. Punch biopsy is suggested as an effective means of removing the organism."}
{"id": "PMID:477387", "title": "Innovative adjuncts in hair transplantation.", "content": "After development of the technical, organizational, and esthetic phases, hair transplantation has progressed to a fourth stage. This is a comprehensive approach determined by the patient's degree of baldness and may require a combination of procedures to best correct the patient's defect. The punch autograft technique remains fundamental for most patients at this time, while scalp reduction, flaps, and various types of grafts may be selective adjuncts. \"Individualization\" is being achieved through these various ancillary modalities.", "contents": "Innovative adjuncts in hair transplantation. After development of the technical, organizational, and esthetic phases, hair transplantation has progressed to a fourth stage. This is a comprehensive approach determined by the patient's degree of baldness and may require a combination of procedures to best correct the patient's defect. The punch autograft technique remains fundamental for most patients at this time, while scalp reduction, flaps, and various types of grafts may be selective adjuncts. \"Individualization\" is being achieved through these various ancillary modalities."}
{"id": "PMID:477388", "title": "The treatment of geographic tongue with topical Retin-A solution.", "content": "A new treatment for geographic tongue with topically applied tretinoin is suggested and its effective use on three patients is described.", "contents": "The treatment of geographic tongue with topical Retin-A solution. A new treatment for geographic tongue with topically applied tretinoin is suggested and its effective use on three patients is described."}
{"id": "PMID:477389", "title": "An unusual organoid nevus.", "content": "The sites of predilection, stages of development, and suggested therapy for organoid nevi are discussed herein, and the case of an unusual nevus is presented.", "contents": "An unusual organoid nevus. The sites of predilection, stages of development, and suggested therapy for organoid nevi are discussed herein, and the case of an unusual nevus is presented."}
{"id": "PMID:477390", "title": "Molluscum contagiosum: the volcano phenomenon.", "content": "Molluscum contagiosum, a common cutaneous viral infection, responds to a variety of therapeutic modalities, but patient cooperation often limits the practitioner's choice. In an effort to provide minimally traumatic treatment for a four year old boy with this dermatosis, an interesting phenomenon was chanced upon, which greatly facilitated therapy.", "contents": "Molluscum contagiosum: the volcano phenomenon. Molluscum contagiosum, a common cutaneous viral infection, responds to a variety of therapeutic modalities, but patient cooperation often limits the practitioner's choice. In an effort to provide minimally traumatic treatment for a four year old boy with this dermatosis, an interesting phenomenon was chanced upon, which greatly facilitated therapy."}
{"id": "PMID:477392", "title": "Atrophie blanche. An unusual ulcerative state of the lower limb.", "content": "A case of atrophie blanche, an unusual ulcerative state of the lower limb, is presented herein.", "contents": "Atrophie blanche. An unusual ulcerative state of the lower limb. A case of atrophie blanche, an unusual ulcerative state of the lower limb, is presented herein."}
{"id": "PMID:477391", "title": "Giant condyloma acuminatum.", "content": "The subtle histologic differences between condyloma acuminatum and giant condyloma acuminatum are presented in the following report.", "contents": "Giant condyloma acuminatum. The subtle histologic differences between condyloma acuminatum and giant condyloma acuminatum are presented in the following report."}
{"id": "PMID:477393", "title": "Acanthoma fissuratum. Histopathology and review of the literature.", "content": "A review of the literature and report of a case describing perhaps the youngest and first black patient recorded in whom bilateral lesions of acanthoma fissuratum developed and were successfully treated with intralesional corticosteroids is presented herein. Awareness of the clinical characteristics of this benign process can prevent potentially costly and needless diagnostic and therapeutic procedures.", "contents": "Acanthoma fissuratum. Histopathology and review of the literature. A review of the literature and report of a case describing perhaps the youngest and first black patient recorded in whom bilateral lesions of acanthoma fissuratum developed and were successfully treated with intralesional corticosteroids is presented herein. Awareness of the clinical characteristics of this benign process can prevent potentially costly and needless diagnostic and therapeutic procedures."}
{"id": "PMID:477399", "title": "Tumor of the follicular infundibulum.", "content": "A histopathologically unique tumor originating from the infundibular portion of vellus hair follicles is presented and the clinical and microscopic features of such a case are described. A superficial subepidermal shelf of trichilemmal structure differentiates the lesion from the lobular acanthosis of trichilemmoma.", "contents": "Tumor of the follicular infundibulum. A histopathologically unique tumor originating from the infundibular portion of vellus hair follicles is presented and the clinical and microscopic features of such a case are described. A superficial subepidermal shelf of trichilemmal structure differentiates the lesion from the lobular acanthosis of trichilemmoma."}
{"id": "PMID:477403", "title": "Regional assignment of human genes TPI1, GAPDH, LDHB, SHMT, and PEPB on chromosome 12.", "content": "Karyological analysis was performed on a series of human-Chinese hamster cell hybrids containing deletions of human chromosome 12. Chromosome breakage was produced by treatment of the cells with either X-rays or 5-bromodeoxyuridine and near-visible light. The hybrid clones were analyzed for the presence or absence of the following five human gene markers known to be located on chromosome 12: triosephosphate isomerase-1 (TPI1),  glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenase-B (LDHB), serine hydroxymethyltransferase (SHMT), and peptidase-B (PEPB). Based on the correlation between the isozyme markers and karyological analysis of these clones, a regional map of the five human genes on chromosome 12 was established. The linear order for these genes is: pter-TPI1-GAPDH-LDHB-centromere-SHMT-PEPB-qter. The locations of these genes are: TPI1, GAPDH, LDHB: pter leads to p12; SHMT: q12 leads to q14; PEPB: q14 leads to qter. Statistical analysis similar to that of Goss and Harris (1975, 1977a, b) has been performed on the segregation data in the hybrid clones. The statistical map, in general, agrees with the cytogenetic map and further localizes PEPB to 12q21.", "contents": "Regional assignment of human genes TPI1, GAPDH, LDHB, SHMT, and PEPB on chromosome 12. Karyological analysis was performed on a series of human-Chinese hamster cell hybrids containing deletions of human chromosome 12. Chromosome breakage was produced by treatment of the cells with either X-rays or 5-bromodeoxyuridine and near-visible light. The hybrid clones were analyzed for the presence or absence of the following five human gene markers known to be located on chromosome 12: triosephosphate isomerase-1 (TPI1),  glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenase-B (LDHB), serine hydroxymethyltransferase (SHMT), and peptidase-B (PEPB). Based on the correlation between the isozyme markers and karyological analysis of these clones, a regional map of the five human genes on chromosome 12 was established. The linear order for these genes is: pter-TPI1-GAPDH-LDHB-centromere-SHMT-PEPB-qter. The locations of these genes are: TPI1, GAPDH, LDHB: pter leads to p12; SHMT: q12 leads to q14; PEPB: q14 leads to qter. Statistical analysis similar to that of Goss and Harris (1975, 1977a, b) has been performed on the segregation data in the hybrid clones. The statistical map, in general, agrees with the cytogenetic map and further localizes PEPB to 12q21."}
{"id": "PMID:477404", "title": "Incidence of nondisjunction in mouse oocytes.", "content": "The chromosomes of more than 3000 ovulated mouse oocytes from strains C3H/Han, NMRI/Han, and (101 X C3H)F1 have been analyzed after spontaneous and hormonally induced ovulation. No significant difference in the incidence of nondisjunction was observed among the three strains with or without hormonal pretreatment. The incidence of nondisjunction was estimated to be 0.47% in NMRI/Han, 0.62% in C3H/Han, and 0.81% in (101 X C3H/F1. The incidence of chromosomal imbalance after the first meiotic division was slightly higher after adding the events following presegregation. Diploidy-spontaneous and hormonally induced-reached a significant leve in NMRI/Han. This may be interpreted as a consequence of hormonal interference with a genetically defined malfunction of gene product(s) during the late phase of oogenesis.", "contents": "Incidence of nondisjunction in mouse oocytes. The chromosomes of more than 3000 ovulated mouse oocytes from strains C3H/Han, NMRI/Han, and (101 X C3H)F1 have been analyzed after spontaneous and hormonally induced ovulation. No significant difference in the incidence of nondisjunction was observed among the three strains with or without hormonal pretreatment. The incidence of nondisjunction was estimated to be 0.47% in NMRI/Han, 0.62% in C3H/Han, and 0.81% in (101 X C3H/F1. The incidence of chromosomal imbalance after the first meiotic division was slightly higher after adding the events following presegregation. Diploidy-spontaneous and hormonally induced-reached a significant leve in NMRI/Han. This may be interpreted as a consequence of hormonal interference with a genetically defined malfunction of gene product(s) during the late phase of oogenesis."}
{"id": "PMID:477409", "title": "Breeding studies reveal segregation of a canine Robertsonian translocation along Mendelian proportions.", "content": "Six matings of golden retriever-type mongrel dogs with a 13/23 Robertsonian translocation were studied. Three matings between normal dogs and dogs heterozygous for the translocation produced 12 normals and 11 heterozygotes. In three matings between heterozygotes, the distribution of normal, heterozygous, and homozygous proteny was Mendelian (8:12:5). No gross phenotypic anomalies were observed with the exception of congenital inguinal hernia in two female homozygotes. Nondisjunction in the form of trisomic or monosomic individuals for either autosome 13 or 23 was not observed.", "contents": "Breeding studies reveal segregation of a canine Robertsonian translocation along Mendelian proportions. Six matings of golden retriever-type mongrel dogs with a 13/23 Robertsonian translocation were studied. Three matings between normal dogs and dogs heterozygous for the translocation produced 12 normals and 11 heterozygotes. In three matings between heterozygotes, the distribution of normal, heterozygous, and homozygous proteny was Mendelian (8:12:5). No gross phenotypic anomalies were observed with the exception of congenital inguinal hernia in two female homozygotes. Nondisjunction in the form of trisomic or monosomic individuals for either autosome 13 or 23 was not observed."}
{"id": "PMID:477408", "title": "The effect of dexamethasone on the karyotype of A9HT cells.", "content": "In the majority of glucocorticoid-resistant receptor containing variants of A9HT cells, the numbers of dot chromosomes was found to be significantly higher than in the parent cells. The higher number of dot chromosomes, however, did not always correlate with the resistance of L cells to dexamethasone. The formation of dot chromosomes was not the consequence of dexamethasone-induced chromosome breaks. Dot-chromosome formation could not be induced by dexamethasone in human diploid cells. It seems likely that dexamethasone can select for A9HT cells with higher number of dot chromosomes.", "contents": "The effect of dexamethasone on the karyotype of A9HT cells. In the majority of glucocorticoid-resistant receptor containing variants of A9HT cells, the numbers of dot chromosomes was found to be significantly higher than in the parent cells. The higher number of dot chromosomes, however, did not always correlate with the resistance of L cells to dexamethasone. The formation of dot chromosomes was not the consequence of dexamethasone-induced chromosome breaks. Dot-chromosome formation could not be induced by dexamethasone in human diploid cells. It seems likely that dexamethasone can select for A9HT cells with higher number of dot chromosomes."}
{"id": "PMID:477412", "title": "Chromosome studies on a human liposarcoma cell line.", "content": "Numerical and structural chromosome analysis of a human retroperitoneal liposarcoma cell line maintained under standard cell culture conditions revealed a very stable hypodiploid mode. If the cells were not trypsinized for several generations, a near-triploid stemline, which was generally a duplication of the hypodiploid mode, emerged. Some chromosomes appeared to be relatively stable pairs (1, 2, 7, 9, and 12), but most had \"lost\" one homolog or both (4 and 21) or were rearranged into \"new\" marker chromosomes. Quantitation of the genetic material showed a loss of 12.0 +/- 3.7% per spread. Only one characteristically long marker chromosome, which is present in every cell, could be identified with certainty as a translocation between chromosomes 4 and 11. Several of the marker chromosomes showed interstitial negatively staining regions with the trypsin-Giemsa method.", "contents": "Chromosome studies on a human liposarcoma cell line. Numerical and structural chromosome analysis of a human retroperitoneal liposarcoma cell line maintained under standard cell culture conditions revealed a very stable hypodiploid mode. If the cells were not trypsinized for several generations, a near-triploid stemline, which was generally a duplication of the hypodiploid mode, emerged. Some chromosomes appeared to be relatively stable pairs (1, 2, 7, 9, and 12), but most had \"lost\" one homolog or both (4 and 21) or were rearranged into \"new\" marker chromosomes. Quantitation of the genetic material showed a loss of 12.0 +/- 3.7% per spread. Only one characteristically long marker chromosome, which is present in every cell, could be identified with certainty as a translocation between chromosomes 4 and 11. Several of the marker chromosomes showed interstitial negatively staining regions with the trypsin-Giemsa method."}
{"id": "PMID:477410", "title": "Pattern of segregation of chicken HPRT phenotype in Chinese hamster-chick red blood cell hybrids.", "content": "The pattern of segregation of hypoxanthine phosphoribosyltransferase (HPRT, E.C. 2.4.2.8) was determined in synchronized Chinese hamster-chick red blood cell hybrids. Three hybrid lines were synchronized at the G1-S boundary. Bromodeoxyuridine pulses were subsequently applied throughout the S phase, and the frequency of the segregant clones was determined. It was found that the segregation of the chicken-specific HPRT phenotype associated with the loss of a chromosome was potentiated by bromodeoxyuridine administered during the first hour following release of the block.", "contents": "Pattern of segregation of chicken HPRT phenotype in Chinese hamster-chick red blood cell hybrids. The pattern of segregation of hypoxanthine phosphoribosyltransferase (HPRT, E.C. 2.4.2.8) was determined in synchronized Chinese hamster-chick red blood cell hybrids. Three hybrid lines were synchronized at the G1-S boundary. Bromodeoxyuridine pulses were subsequently applied throughout the S phase, and the frequency of the segregant clones was determined. It was found that the segregation of the chicken-specific HPRT phenotype associated with the loss of a chromosome was potentiated by bromodeoxyuridine administered during the first hour following release of the block."}
{"id": "PMID:477413", "title": "Studies on isolated cloned populations from irradiated human embryonic cell cultures.", "content": "The recovery of substrains with stable chromosome aberrations from irradiated fibroblast culture are reported. Four human fetal cell strains were exposed to 600 rad of gamma rays at 200 rad/min. The efficiency of recovering viable cloned subpopulations was approximately 87%, and the frequency of clones with abnormal chromosomes was 40/100 colonies. G-band chromosome analyses for 34 abnormal substrains are described. Karyotypes of some of the clones with complex rearrangements are also presented. Analyses of a total of 47 aberrant events in the 34 abnormal substrains revealed at 7:1 and a 9:1 translocation-inversion and translocation-deletion ratios, respectively. Five of the abnormal substrains were continuously cultured; all except one showed signs of sensecence toward the end of 44 +/- 10 doublings. Unusual prolonged proliferation capacity was observed in substrain FFS-1-9. The significance of this finding is discussed.", "contents": "Studies on isolated cloned populations from irradiated human embryonic cell cultures. The recovery of substrains with stable chromosome aberrations from irradiated fibroblast culture are reported. Four human fetal cell strains were exposed to 600 rad of gamma rays at 200 rad/min. The efficiency of recovering viable cloned subpopulations was approximately 87%, and the frequency of clones with abnormal chromosomes was 40/100 colonies. G-band chromosome analyses for 34 abnormal substrains are described. Karyotypes of some of the clones with complex rearrangements are also presented. Analyses of a total of 47 aberrant events in the 34 abnormal substrains revealed at 7:1 and a 9:1 translocation-inversion and translocation-deletion ratios, respectively. Five of the abnormal substrains were continuously cultured; all except one showed signs of sensecence toward the end of 44 +/- 10 doublings. Unusual prolonged proliferation capacity was observed in substrain FFS-1-9. The significance of this finding is discussed."}
{"id": "PMID:477414", "title": "Retinoblastoma, chromosome 13, and in vitro cellular radiosensitivity.", "content": "Five diploid fibroblast strains from patients with deletions mapping in the long arm of chromosome 13 and three strains bearing partial trisomies of chromosome 13 were assayed for clonogenic survival following X-irradiation. Results of these experiments suggest the existence of a region on 13q14 which is related to increased sensitivity to cell killing in vitro by X-rays. This locus seems to be distinct from but close to the retinoblastoma locus, which has been associated with the same band.", "contents": "Retinoblastoma, chromosome 13, and in vitro cellular radiosensitivity. Five diploid fibroblast strains from patients with deletions mapping in the long arm of chromosome 13 and three strains bearing partial trisomies of chromosome 13 were assayed for clonogenic survival following X-irradiation. Results of these experiments suggest the existence of a region on 13q14 which is related to increased sensitivity to cell killing in vitro by X-rays. This locus seems to be distinct from but close to the retinoblastoma locus, which has been associated with the same band."}
{"id": "PMID:477411", "title": "Assignment of a gene for uridine diphosphate galactose-4-epimerase to human chromosome 1 by somatic cell hybridization, with evidence for a regional assignment to 1pter yields 1p21.", "content": "The presence of human uridine diphosphate galactose-4-epimerase (GALE) was found to correlate with the presence of chromosome 1 in somatic cell hybrids between man and mouse. The gene for GALE can therefore be assigned to human chromosome 1. Using a chromosome 1 rearrangement, we have been able to regionally assign GALE to the pter yields p21 region.", "contents": "Assignment of a gene for uridine diphosphate galactose-4-epimerase to human chromosome 1 by somatic cell hybridization, with evidence for a regional assignment to 1pter yields 1p21. The presence of human uridine diphosphate galactose-4-epimerase (GALE) was found to correlate with the presence of chromosome 1 in somatic cell hybrids between man and mouse. The gene for GALE can therefore be assigned to human chromosome 1. Using a chromosome 1 rearrangement, we have been able to regionally assign GALE to the pter yields p21 region."}
{"id": "PMID:477425", "title": "Progression of aortic stenosis.", "content": "We assessed progressive stenosis of the aortic valve in 11 adult patients (mean age of 48 years) with aortic stenosis who had undergone two cardiac catheterizations without intervening aortic valve surgery. The mean time between cardiac catheterization was 59 months (range 20 to 133). No patients had mitral valve disease. Two patients had coronary artery disease. The results showed that progressive stenosis of the aortic valve occurred in 10 of 11 patients with a significant decrease in the calculated mean aortic valve area from 1.2 +/- 0.2 sq cm to 0.7 +/- 0.1 sq cm (P less than 0.005); a significant increase in mean left ventricular peak systolic pressure from 149 +/- 8 mm Hg to 199 +/- 3 mm Hg (P less than 0.01), and a significant increase in mean left ventricular aortic pressure gradient from 31 +/- 4 mm Hg to 75 +/- 13 mm Hg (P less than 0.005). On an individual basis, the change in left ventricular pressure and the left ventricular-aortic gradient did not always reflect the decrease in aortic valve area because of variations in cardiac output. The shortest period of time in which progression of aortic stenosis occurred was 27 to 29 months. Thus, progressive stenosis of the aortic valve occurs in adults with isolated aortic valvular stenosis. Significant decrease in the aortic valve area can develop in as short a period as 27 to 29 months.", "contents": "Progression of aortic stenosis. We assessed progressive stenosis of the aortic valve in 11 adult patients (mean age of 48 years) with aortic stenosis who had undergone two cardiac catheterizations without intervening aortic valve surgery. The mean time between cardiac catheterization was 59 months (range 20 to 133). No patients had mitral valve disease. Two patients had coronary artery disease. The results showed that progressive stenosis of the aortic valve occurred in 10 of 11 patients with a significant decrease in the calculated mean aortic valve area from 1.2 +/- 0.2 sq cm to 0.7 +/- 0.1 sq cm (P less than 0.005); a significant increase in mean left ventricular peak systolic pressure from 149 +/- 8 mm Hg to 199 +/- 3 mm Hg (P less than 0.01), and a significant increase in mean left ventricular aortic pressure gradient from 31 +/- 4 mm Hg to 75 +/- 13 mm Hg (P less than 0.005). On an individual basis, the change in left ventricular pressure and the left ventricular-aortic gradient did not always reflect the decrease in aortic valve area because of variations in cardiac output. The shortest period of time in which progression of aortic stenosis occurred was 27 to 29 months. Thus, progressive stenosis of the aortic valve occurs in adults with isolated aortic valvular stenosis. Significant decrease in the aortic valve area can develop in as short a period as 27 to 29 months."}
{"id": "PMID:477424", "title": "Variability in interpretation of pulmonary function tests.", "content": "We asked 26 pulmonary physicians to interpret results of ten consecutive pulmonary function tests from one laboratory. There was frequent disagreement in their assessment of respiratory impairment. This is likely due to the variety of criteria for grading the severity of obstructive and restrictive defects. Since the narrative interpretation of pulmonary function data may influence patient care, physicians should consider the numerical magnitude of the derangements, as well as the descriptive terms applied.", "contents": "Variability in interpretation of pulmonary function tests. We asked 26 pulmonary physicians to interpret results of ten consecutive pulmonary function tests from one laboratory. There was frequent disagreement in their assessment of respiratory impairment. This is likely due to the variety of criteria for grading the severity of obstructive and restrictive defects. Since the narrative interpretation of pulmonary function data may influence patient care, physicians should consider the numerical magnitude of the derangements, as well as the descriptive terms applied."}
{"id": "PMID:477423", "title": "Spirometric testing. Number of tests required and selection of data.", "content": "To address the questions of whether three or five maneuvers for forced vital capacity (FVC) should be required for adequate spirometric testing and whether the largest or mean values for FVC and the forced expiratory volume in the first second (FEV1) should be used for analysis, we analyzed spirometric data obtained from approximately 3,000 people for each of three consecutive years. The following four algorithms for selection of data were tested: (1) the average of the best two of five measurements; (2) the average of the best two of the first three; (3) the best of five; and (4) the best of the first three. There were no significant differences between any two algorithms within a given year and no differences between any two years for any algorithm. We conclude that there is little to be gained by requiring more than three maneuvers for FVC or in using values other than the largest FEV1 and FVC.", "contents": "Spirometric testing. Number of tests required and selection of data. To address the questions of whether three or five maneuvers for forced vital capacity (FVC) should be required for adequate spirometric testing and whether the largest or mean values for FVC and the forced expiratory volume in the first second (FEV1) should be used for analysis, we analyzed spirometric data obtained from approximately 3,000 people for each of three consecutive years. The following four algorithms for selection of data were tested: (1) the average of the best two of five measurements; (2) the average of the best two of the first three; (3) the best of five; and (4) the best of the first three. There were no significant differences between any two algorithms within a given year and no differences between any two years for any algorithm. We conclude that there is little to be gained by requiring more than three maneuvers for FVC or in using values other than the largest FEV1 and FVC."}
{"id": "PMID:477422", "title": "Pulmonary hemodynamics in systemic hypertension. Long-term effect of minoxidil.", "content": "To assess the effect of minoxidil on pulmonary hemodynamics, we performed cardiac catheterization on ten patients, prior to the administration of minoxidil, at six months after its addition to their existing drug regimens, and on seven patients following 12 or more months of minoxidil therapy. Systemic blood pressure and systemic vascular resistance were significantly reduced at six months and following 12 or more months of minoxidil therapy. Before receiving minoxidil, nine of ten patients had elevated pulmonary vascular resistance. There were no significant changes in pulmonary vascular resistance following the initiation of minoxidil. The data suggest that pulmonary hypertension is common in patients with long-standing poorly controlled systemic hypertension, and that minoxidil, in doses sufficient to significantly reduce systemic blood pressure, does not cause or aggravate pulmonary hypertension.", "contents": "Pulmonary hemodynamics in systemic hypertension. Long-term effect of minoxidil. To assess the effect of minoxidil on pulmonary hemodynamics, we performed cardiac catheterization on ten patients, prior to the administration of minoxidil, at six months after its addition to their existing drug regimens, and on seven patients following 12 or more months of minoxidil therapy. Systemic blood pressure and systemic vascular resistance were significantly reduced at six months and following 12 or more months of minoxidil therapy. Before receiving minoxidil, nine of ten patients had elevated pulmonary vascular resistance. There were no significant changes in pulmonary vascular resistance following the initiation of minoxidil. The data suggest that pulmonary hypertension is common in patients with long-standing poorly controlled systemic hypertension, and that minoxidil, in doses sufficient to significantly reduce systemic blood pressure, does not cause or aggravate pulmonary hypertension."}
{"id": "PMID:477426", "title": "The radiologic significance of the left pulmonary ligament. Experience with 26 patients.", "content": "Pathologic processes confined to or by the left pulmonary ligament present a confusing radiographic appearance. Such processes may simulate pleural scarring, parenchymal scarring, or even left lower lobe collapse. Radiologic awareness of this structure is limited because in the normal state, it is not visualized on either posteroanterior or lateral chest x-ray films. The absence of secondary signs of left lower lobe collapse, together with a process which may extend above the level of the left hilum, is valuable in confirming that such a triangular density seen behind the left cardiac border is disease in or confined by the pulmonary ligament, rather than left lower lobe collapse.", "contents": "The radiologic significance of the left pulmonary ligament. Experience with 26 patients. Pathologic processes confined to or by the left pulmonary ligament present a confusing radiographic appearance. Such processes may simulate pleural scarring, parenchymal scarring, or even left lower lobe collapse. Radiologic awareness of this structure is limited because in the normal state, it is not visualized on either posteroanterior or lateral chest x-ray films. The absence of secondary signs of left lower lobe collapse, together with a process which may extend above the level of the left hilum, is valuable in confirming that such a triangular density seen behind the left cardiac border is disease in or confined by the pulmonary ligament, rather than left lower lobe collapse."}
{"id": "PMID:477427", "title": "Necrotizing sarcoid granulomatosis.", "content": "Twelve cases of necrotizing sarcoid granulomatosis are presented as a retrospective study. The population of patients consisted of ten women and two men, with an average age of 50 years. Nine patients initially had a variety of pulmonary and nonpulmonary complaints, while three were asymptomatic. Chest roentgenograms demonstrated bilateral nodules in seven patients, solitary nodules in four, and a miliary pattern progressing to nodules in one. Enlarged hilar nodes were found in six. Histologically, all biopsies showed a combination of individual granulomas, confluent masses of granulomas which formed the nodular masses seen on the roentgenograms, a variable amount of necrosis of tissue and hyalinization, and a granulomatous vasculitis. Follow-up periods ranged from four months to 11 years. Eleven patients are alive and asymptomatic. The sole death occurred in a patient treated with an immunosuppressive agent (cyclophosphamide). We conclude that the clinical behavior of necrotizing sarcoid granulomatosis is not similar to that of the other angiocentric granulomatoses and that most patients with this disease can be left untreated or be treated with steroids alone. We suggest the possibility that necrotizing sarcoid granulomatosis may be the histologic counterpart of so-called nodular sarcoid.", "contents": "Necrotizing sarcoid granulomatosis. Twelve cases of necrotizing sarcoid granulomatosis are presented as a retrospective study. The population of patients consisted of ten women and two men, with an average age of 50 years. Nine patients initially had a variety of pulmonary and nonpulmonary complaints, while three were asymptomatic. Chest roentgenograms demonstrated bilateral nodules in seven patients, solitary nodules in four, and a miliary pattern progressing to nodules in one. Enlarged hilar nodes were found in six. Histologically, all biopsies showed a combination of individual granulomas, confluent masses of granulomas which formed the nodular masses seen on the roentgenograms, a variable amount of necrosis of tissue and hyalinization, and a granulomatous vasculitis. Follow-up periods ranged from four months to 11 years. Eleven patients are alive and asymptomatic. The sole death occurred in a patient treated with an immunosuppressive agent (cyclophosphamide). We conclude that the clinical behavior of necrotizing sarcoid granulomatosis is not similar to that of the other angiocentric granulomatoses and that most patients with this disease can be left untreated or be treated with steroids alone. We suggest the possibility that necrotizing sarcoid granulomatosis may be the histologic counterpart of so-called nodular sarcoid."}
{"id": "PMID:477428", "title": "Doxapram hydrochloride: a respiratory stimulant for patients with primary alveolar hypoventilation.", "content": "Four patients (ages 43 to 51) with primary alveolar hypoventilation (PAH) syndrome were studied to characterize the pharmacologic augmentation of ventilation with intravenous doxapram hydrochloride. Doxapram hydrochloride evoked a rapid ventilatory increase of 50 to 100 percent in all four subjects with a consequent decrease in arterial CO2 tension. Blood pressure and heart rate measurements showed small increases during the doxapram infusion. These responses, however, were only sustained during the infusion, decreasing to their predoxapram level when the drug was discontinued. The ventilatory response to the drug was more marked in the pressence of hypoxia than during high O2 breathing, suggesting that the carotid bodies are a site of action for this drug in man. Doxapram hydrochloride can be an effective respiratory stimulant in patients with PAH.", "contents": "Doxapram hydrochloride: a respiratory stimulant for patients with primary alveolar hypoventilation. Four patients (ages 43 to 51) with primary alveolar hypoventilation (PAH) syndrome were studied to characterize the pharmacologic augmentation of ventilation with intravenous doxapram hydrochloride. Doxapram hydrochloride evoked a rapid ventilatory increase of 50 to 100 percent in all four subjects with a consequent decrease in arterial CO2 tension. Blood pressure and heart rate measurements showed small increases during the doxapram infusion. These responses, however, were only sustained during the infusion, decreasing to their predoxapram level when the drug was discontinued. The ventilatory response to the drug was more marked in the pressence of hypoxia than during high O2 breathing, suggesting that the carotid bodies are a site of action for this drug in man. Doxapram hydrochloride can be an effective respiratory stimulant in patients with PAH."}
{"id": "PMID:477429", "title": "Bacterial colonization, tracheobronchitis, and pneumonia following tracheostomy and long-term intubation in pediatric patients.", "content": "Serial tracheal cultures for aerobic and anaerobic bacteria were obtained from 27 pediatric patients during one year of follow-up. The patients had required tracheostomy and prolonged intubation for periods ranging from 3 to 12 months (average, 7 1/2 months). Cultures of tracheal aspirates yielded 1,508 isolates of pathogenic aerobic (969 isolates) and anaerobic (539 isolates) bacteria. The most frequent aerobic isolates were Streptococcus pneumoniae and Staphylococcus aureus. The predominant anaerobes were anaerobic gram-positive cocci, Fusobacterium nucleatum, and Bacteroides fragilis. Replacement of one pathogen by another occurred frequently. Tracheobronchitis occurred in 24 patients, all of whom had episodes of pneumonia. The data suggest that anaerobic bacteria are a part of the bacterial flora in colonization, tracheobronchitis, and pneumonia in patients with tracheostomy and prolonged intubation.", "contents": "Bacterial colonization, tracheobronchitis, and pneumonia following tracheostomy and long-term intubation in pediatric patients. Serial tracheal cultures for aerobic and anaerobic bacteria were obtained from 27 pediatric patients during one year of follow-up. The patients had required tracheostomy and prolonged intubation for periods ranging from 3 to 12 months (average, 7 1/2 months). Cultures of tracheal aspirates yielded 1,508 isolates of pathogenic aerobic (969 isolates) and anaerobic (539 isolates) bacteria. The most frequent aerobic isolates were Streptococcus pneumoniae and Staphylococcus aureus. The predominant anaerobes were anaerobic gram-positive cocci, Fusobacterium nucleatum, and Bacteroides fragilis. Replacement of one pathogen by another occurred frequently. Tracheobronchitis occurred in 24 patients, all of whom had episodes of pneumonia. The data suggest that anaerobic bacteria are a part of the bacterial flora in colonization, tracheobronchitis, and pneumonia in patients with tracheostomy and prolonged intubation."}
{"id": "PMID:477430", "title": "Determination of static pulmonary volumes after bronchodilator therapy.", "content": "Increased (more positive) end-expiratory and decreased (more negative) end-inspiratory values for intrapleural pressure (PpI) invariably accompany acute bronchoconstriction. We hypothesize that both the increase in vital capacity (VC) and the decrease in residual volume (RV) observed after dilation of the central airways in patients with reversible obstruction of the airways result, in part, from a restoration of normal PpI during unforced exhalation. To test this hypothesis, we examined the end-expiratory PpI during breathing at rest in ten emphysematous and eight asthmatic subjects before and after inhalation of isoproterenol. The VC increased by 0.38 L after therapy, and the specific airway resistance and the RV decreased by 6.8 cm H2O.sec and 0.63 L, respectively. Total lung capacity was unchanged. The response of the VC to administration of isoproterenol is an important sequel to dilation of the large airways. Bronchioles close at a critical PpI during exhalation. Because PpI normalizes with administration of isoproterenol, this closure may be delayed to a lower pulmonary volume even if improvement in the function of peripheral airways does not occur.", "contents": "Determination of static pulmonary volumes after bronchodilator therapy. Increased (more positive) end-expiratory and decreased (more negative) end-inspiratory values for intrapleural pressure (PpI) invariably accompany acute bronchoconstriction. We hypothesize that both the increase in vital capacity (VC) and the decrease in residual volume (RV) observed after dilation of the central airways in patients with reversible obstruction of the airways result, in part, from a restoration of normal PpI during unforced exhalation. To test this hypothesis, we examined the end-expiratory PpI during breathing at rest in ten emphysematous and eight asthmatic subjects before and after inhalation of isoproterenol. The VC increased by 0.38 L after therapy, and the specific airway resistance and the RV decreased by 6.8 cm H2O.sec and 0.63 L, respectively. Total lung capacity was unchanged. The response of the VC to administration of isoproterenol is an important sequel to dilation of the large airways. Bronchioles close at a critical PpI during exhalation. Because PpI normalizes with administration of isoproterenol, this closure may be delayed to a lower pulmonary volume even if improvement in the function of peripheral airways does not occur."}
{"id": "PMID:477431", "title": "Failure of surgery in preexcitation. Correlation with pathologic findings.", "content": "A 37-year-old man with mitral stenosis and recurrent drug-resistant paroxysmal atrial fibrillation, paroxysmal supraventricular tachycardia, and preexcitation, underwent two surgical attempts to ablate an anomalous pathway (AP). Electrophysiologic study demonstrated a left posterior AP with a short antegrade refractory period. Epicardial mapping at the time of mitral valve replacement (left lateral thoracotomy) suggested a posterior right AP. Mitral valve replacement and incision of the left atrial wall failed to cure preexcitation. Epicardial mapping at a second operation (median sternotomy) demonstrated a subepicardial left posterior AP. Right atrial and atrial septal incisions failed to cure preexcitation. Serial section of the atrioventricular rings and conduction system demonstrated an intact left posterior anomalous atrioventricular muscle bundle with surgical incision placed above the plane of the mitral anulus.", "contents": "Failure of surgery in preexcitation. Correlation with pathologic findings. A 37-year-old man with mitral stenosis and recurrent drug-resistant paroxysmal atrial fibrillation, paroxysmal supraventricular tachycardia, and preexcitation, underwent two surgical attempts to ablate an anomalous pathway (AP). Electrophysiologic study demonstrated a left posterior AP with a short antegrade refractory period. Epicardial mapping at the time of mitral valve replacement (left lateral thoracotomy) suggested a posterior right AP. Mitral valve replacement and incision of the left atrial wall failed to cure preexcitation. Epicardial mapping at a second operation (median sternotomy) demonstrated a subepicardial left posterior AP. Right atrial and atrial septal incisions failed to cure preexcitation. Serial section of the atrioventricular rings and conduction system demonstrated an intact left posterior anomalous atrioventricular muscle bundle with surgical incision placed above the plane of the mitral anulus."}
{"id": "PMID:477432", "title": "Tricuspid and pulmonic valve echoes in tricuspid and pulmonary atresia.", "content": "An echocardiographic pattern of normal tricuspid valve motion in a patient with tricuspid atresia, and pulmonic valve motion in a patient with transposition of the great vessels and pulmonary atresia were documented. The recordings of the valve motion resulted in an initial erroneous diagnosis. Although M-mode echocardiography is very useful for the initial diagnostic evaluation of critically ill newborn infants with complex congenital heart diseases, on occasion recording of valvular echoes may result in conflicting clinical and pathologic correlation.", "contents": "Tricuspid and pulmonic valve echoes in tricuspid and pulmonary atresia. An echocardiographic pattern of normal tricuspid valve motion in a patient with tricuspid atresia, and pulmonic valve motion in a patient with transposition of the great vessels and pulmonary atresia were documented. The recordings of the valve motion resulted in an initial erroneous diagnosis. Although M-mode echocardiography is very useful for the initial diagnostic evaluation of critically ill newborn infants with complex congenital heart diseases, on occasion recording of valvular echoes may result in conflicting clinical and pathologic correlation."}
{"id": "PMID:477433", "title": "Echocardiographic indices of left ventricular function. A comparison.", "content": "Multiple M-mode echocardiographic indices of left ventricular function are currently in use, but their reliability is disputed. Therefore, we correlated echo ejection fraction (EF) using quadratic (Q) and cubic (C) formulae, percentage of minor axis shortening (%S), mean velocity of circumferential fiber shortening (mVCF), and peak VCF with angiographic EF in 37 subjects, including 10 normal subjects. None had echocardiographic segmental wall motion abnormalities. Significant linear correlations were found between angiographic EF and EFc (r = 0.78), %S (r = 0.77), EFQ(r = 0.68), peak VCF (r = 0.68), and mVCF (r = 0.58). However, for all indices, the scatter was too great to permit reliable quantitative estimations of angiographic EF based on echo date. Peak VCF was qualitatively the most reliable predictor of ventricular function, being greater than or equal to 2.00 circumference per second in 23 of 25 patients with angio EF greater than or equal to 55 percent and less than 2.00 circumferences per second in 11 of 12 with EF less than 55 (sensitivity [sens] 92 percent, specificity [spec] 92 percent). Mean VCF (sens 83 percent, spec 80 percent), %S (sens 50 percent, spec 96 percent), EFC (senc 58 percent, spec 96 percent), and EFQ (sens 58 percent, spec 92 percent) were less satisfactory. We conclude that echo indices are reliable only as qualitative parameters of ventricular function. Peak VCF appears to be the most sensitive and specific qualitative index now available.", "contents": "Echocardiographic indices of left ventricular function. A comparison. Multiple M-mode echocardiographic indices of left ventricular function are currently in use, but their reliability is disputed. Therefore, we correlated echo ejection fraction (EF) using quadratic (Q) and cubic (C) formulae, percentage of minor axis shortening (%S), mean velocity of circumferential fiber shortening (mVCF), and peak VCF with angiographic EF in 37 subjects, including 10 normal subjects. None had echocardiographic segmental wall motion abnormalities. Significant linear correlations were found between angiographic EF and EFc (r = 0.78), %S (r = 0.77), EFQ(r = 0.68), peak VCF (r = 0.68), and mVCF (r = 0.58). However, for all indices, the scatter was too great to permit reliable quantitative estimations of angiographic EF based on echo date. Peak VCF was qualitatively the most reliable predictor of ventricular function, being greater than or equal to 2.00 circumference per second in 23 of 25 patients with angio EF greater than or equal to 55 percent and less than 2.00 circumferences per second in 11 of 12 with EF less than 55 (sensitivity [sens] 92 percent, specificity [spec] 92 percent). Mean VCF (sens 83 percent, spec 80 percent), %S (sens 50 percent, spec 96 percent), EFC (senc 58 percent, spec 96 percent), and EFQ (sens 58 percent, spec 92 percent) were less satisfactory. We conclude that echo indices are reliable only as qualitative parameters of ventricular function. Peak VCF appears to be the most sensitive and specific qualitative index now available."}
{"id": "PMID:477434", "title": "Relation between pulmonary transvascular fluid filtration rate and measured Starling's forces after major burn.", "content": "Our purpose was to determine the reliability of the currently used measurements of pulmonary vascular hydrostatic and oncotic pressures in monitoring pulmonary water after a major burn. The flow of pulmonary lymph, a reliable indicator of the rate of filtration of pulmonary transvascular fluid (Qf), was measured before and for 72 hours after a third-degree burn over 40 to 55 percent of the total body surface in sheep. Changes in Qf were correlated with measured changes in pulmonary microvascular (Pmv), plasma colloid osmotic pressure (pi p), and interstitial colloid osmotic pressure (pi i), as measured in the pulmonary lymph. The periods from 0 to 24 hours (resuscitation), from 24 to 48 hours (early mobilization of fluid), and from 48 to 72 hours (early recovery) were compared with baseline. Regression equations and correlation coefficients for Qf vs Pmv, Qf vs Pmv minus pi p, and Qf vs Pmv minus (pi p -ph i), the oncotic gradient, were calculated. There were significant differences in slopes between the periods of time, with all three comparisons indicating that the response of the microcirculation to the same changes in pressure was different in each of the periods. The correlation between Qf and the comparisons of pressures was clearly best in the period from 48 to 72 hours. The comparison of Pmv minus pi p was not better than Pmv alone, while the comparison of Pmv minus the gradient (pi p -- pi i) was significantly better than either of the other comparisons in predicting Qf.", "contents": "Relation between pulmonary transvascular fluid filtration rate and measured Starling's forces after major burn. Our purpose was to determine the reliability of the currently used measurements of pulmonary vascular hydrostatic and oncotic pressures in monitoring pulmonary water after a major burn. The flow of pulmonary lymph, a reliable indicator of the rate of filtration of pulmonary transvascular fluid (Qf), was measured before and for 72 hours after a third-degree burn over 40 to 55 percent of the total body surface in sheep. Changes in Qf were correlated with measured changes in pulmonary microvascular (Pmv), plasma colloid osmotic pressure (pi p), and interstitial colloid osmotic pressure (pi i), as measured in the pulmonary lymph. The periods from 0 to 24 hours (resuscitation), from 24 to 48 hours (early mobilization of fluid), and from 48 to 72 hours (early recovery) were compared with baseline. Regression equations and correlation coefficients for Qf vs Pmv, Qf vs Pmv minus pi p, and Qf vs Pmv minus (pi p -ph i), the oncotic gradient, were calculated. There were significant differences in slopes between the periods of time, with all three comparisons indicating that the response of the microcirculation to the same changes in pressure was different in each of the periods. The correlation between Qf and the comparisons of pressures was clearly best in the period from 48 to 72 hours. The comparison of Pmv minus pi p was not better than Pmv alone, while the comparison of Pmv minus the gradient (pi p -- pi i) was significantly better than either of the other comparisons in predicting Qf."}
{"id": "PMID:477439", "title": "Pericardial effusions in sarcoidosis.", "content": "A case of sarcoid pericarditis is presented. The associated pericardial effusion was a transudate with a low complement level. Investigating the prevalence of pericardial effusions in sarcoid by echocardiography, we found small effusions in 19 percent of 48 consecutive patients with sarcoid. Additionally, ten previously reported cases of symptomatic parietal pericardial sarcoid are reviewed.", "contents": "Pericardial effusions in sarcoidosis. A case of sarcoid pericarditis is presented. The associated pericardial effusion was a transudate with a low complement level. Investigating the prevalence of pericardial effusions in sarcoid by echocardiography, we found small effusions in 19 percent of 48 consecutive patients with sarcoid. Additionally, ten previously reported cases of symptomatic parietal pericardial sarcoid are reviewed."}
{"id": "PMID:477440", "title": "Swallowing-induced supraventricular tachycardia.", "content": "A rare form of paroxysmal supraventricular tachycardia (SVT), associated with swallowing solid food, is described. Repeated triggering of the arrhythmia during esophagoscopy was produced by mechanical stimulation of the anterior esophageal wall, 30 cm from the incisors. Treatment with verapamil abolished this tachycardia. Esophagoscopic examination, the first of its kind in this setting, aided the investigation.", "contents": "Swallowing-induced supraventricular tachycardia. A rare form of paroxysmal supraventricular tachycardia (SVT), associated with swallowing solid food, is described. Repeated triggering of the arrhythmia during esophagoscopy was produced by mechanical stimulation of the anterior esophageal wall, 30 cm from the incisors. Treatment with verapamil abolished this tachycardia. Esophagoscopic examination, the first of its kind in this setting, aided the investigation."}
{"id": "PMID:477441", "title": "Elimination of Mycobacterium intracellulare from sputum after bronchial hygiene.", "content": "Three cases are presented, each of which had more than 20 positive cultures of sputum for Mycobacterium intracellulare, infiltrations, and a history of bronchiectasis. Two had complete and the third nearly complete, sputum conversion during a program of bronchial hygiene without chemotherapy. It is probable that these cases were only noninvasive colonizations in previously diseases lungs. Recommendations are given for a therapeutic approach in such cases.", "contents": "Elimination of Mycobacterium intracellulare from sputum after bronchial hygiene. Three cases are presented, each of which had more than 20 positive cultures of sputum for Mycobacterium intracellulare, infiltrations, and a history of bronchiectasis. Two had complete and the third nearly complete, sputum conversion during a program of bronchial hygiene without chemotherapy. It is probable that these cases were only noninvasive colonizations in previously diseases lungs. Recommendations are given for a therapeutic approach in such cases."}
{"id": "PMID:477442", "title": "Disseminated echinococcosis with repeated anaphylactic shock. Treated with mebendazole.", "content": "A 46-year-old man, who was twice operated on for echinococcosis and who suffered from recurrent angioneurotic edema, was hospitalized with anaphylactic shock. Chest x-ray examination showed round lesions in the lungs; Casoni, immunofluorescence and hemagglutination tests for echinococcosis were strongly positive. With a diagnosis of disseminated echinococcosis, drug treatment with mebenzadole was given for 29 weeks (the last 22 weeks a dose of 1,200 mg daily). No side effects were observed, allergic manifestations disappeared, and the round lesions were noticeably reduced. The titer of hemagglutination fell gradually, and IgE concentration initially increased and later fell. Mebendazole is proposed as a drug of choice in the cure of disseminated hydatidosis; it may both alter the indication for operation and considerably improve the prognosis.", "contents": "Disseminated echinococcosis with repeated anaphylactic shock. Treated with mebendazole. A 46-year-old man, who was twice operated on for echinococcosis and who suffered from recurrent angioneurotic edema, was hospitalized with anaphylactic shock. Chest x-ray examination showed round lesions in the lungs; Casoni, immunofluorescence and hemagglutination tests for echinococcosis were strongly positive. With a diagnosis of disseminated echinococcosis, drug treatment with mebenzadole was given for 29 weeks (the last 22 weeks a dose of 1,200 mg daily). No side effects were observed, allergic manifestations disappeared, and the round lesions were noticeably reduced. The titer of hemagglutination fell gradually, and IgE concentration initially increased and later fell. Mebendazole is proposed as a drug of choice in the cure of disseminated hydatidosis; it may both alter the indication for operation and considerably improve the prognosis."}
{"id": "PMID:477443", "title": "Partial right-sided congenital pericardial defect with herniation of right atrium and right ventricle.", "content": "In an unusual case of right-sided pericardial defect with herniation of the right atrium and right ventricle, cardiac blood pool isotope imaging is introduced as a new, noninvasive diagnostic procedure. Surgery which consisted of excision of right-sided pericardium relieved the chest pain which was the only symptom the patient had prior to surgery.", "contents": "Partial right-sided congenital pericardial defect with herniation of right atrium and right ventricle. In an unusual case of right-sided pericardial defect with herniation of the right atrium and right ventricle, cardiac blood pool isotope imaging is introduced as a new, noninvasive diagnostic procedure. Surgery which consisted of excision of right-sided pericardium relieved the chest pain which was the only symptom the patient had prior to surgery."}
{"id": "PMID:477444", "title": "Intracoronary anastomosis in the absence of obstructive lesions of the coronary arteries.", "content": "The patient had collateral flow from the right coronary to left circumflex coronary artery in the absence of proximal obstructive disease. This supports the hypothesis that the intercoronary collaterals seen in coronary atherosclerotic disease are present in the normal heart.", "contents": "Intracoronary anastomosis in the absence of obstructive lesions of the coronary arteries. The patient had collateral flow from the right coronary to left circumflex coronary artery in the absence of proximal obstructive disease. This supports the hypothesis that the intercoronary collaterals seen in coronary atherosclerotic disease are present in the normal heart."}
{"id": "PMID:477452", "title": "Concentration of thiamphenicol in the human prostate and testis.", "content": "The concentration of thiamphenicol in prostatic tissue, testicular tissue and serum after a single intravenous injection of 1 g thiamphenicol glycinate ester was investigated. The prostatic and testicular specimens were obtained by prostatectomy and plastic orchidectomy, respectively, from patients with adenomas and carcinomas of the prostate. 2 h after dosing, the prostatic tissue concentration ranged from 2.1 to 15.1 microgram/g (mean value 5.7 microgram/g) and the testicular tissue from 3.4 to 8.4 microgram/g (mean value 6.1 microgram/g). At the same time the thiamphenicol serum levels varied in the patients with prostate adenomas from 4.6 to 14.5 microgram/ml (mean 8.9 microgram/ml) and in the prostate carcinoma patients from 5.2 to 10.4 microgram/ml (mean 8.5 microgram/ml). Several factors influencing the diffusion of thiamphenicol into the prostate and testis are discussed. The levels of thiamphenicol in the prostate and testis suggest that the antibiotic may be valuable for the treatment of infections of the prostate and testis caused by gram-positive and gram-negative cocci, but is of questionable value for the treatment of infections caused by gram-negative bacilli.", "contents": "Concentration of thiamphenicol in the human prostate and testis. The concentration of thiamphenicol in prostatic tissue, testicular tissue and serum after a single intravenous injection of 1 g thiamphenicol glycinate ester was investigated. The prostatic and testicular specimens were obtained by prostatectomy and plastic orchidectomy, respectively, from patients with adenomas and carcinomas of the prostate. 2 h after dosing, the prostatic tissue concentration ranged from 2.1 to 15.1 microgram/g (mean value 5.7 microgram/g) and the testicular tissue from 3.4 to 8.4 microgram/g (mean value 6.1 microgram/g). At the same time the thiamphenicol serum levels varied in the patients with prostate adenomas from 4.6 to 14.5 microgram/ml (mean 8.9 microgram/ml) and in the prostate carcinoma patients from 5.2 to 10.4 microgram/ml (mean 8.5 microgram/ml). Several factors influencing the diffusion of thiamphenicol into the prostate and testis are discussed. The levels of thiamphenicol in the prostate and testis suggest that the antibiotic may be valuable for the treatment of infections of the prostate and testis caused by gram-positive and gram-negative cocci, but is of questionable value for the treatment of infections caused by gram-negative bacilli."}
{"id": "PMID:477453", "title": "On the mechanism of the antimycobacterial activity of isoniazid + prothionamide + dapsone (Isoprodian).", "content": "Increased antimycobacterial activity of Isoprodian (isoniazid + prothionamide + dapsone) may be due to (i) decreased mutation rate for INH resistance provoked in mycobacteria by DDS (in 2 of 3 strains tested); (ii) leakage of K+, Na+ or Ca++ caused by INH and/or by PTH (in all 3 strains tested), and (iii) indicating damages which may increase the low level penetration of DDS into the cell in sub-MIC concentrations as shown by phage proliferation changes (tested in 1 strain with 1 phage).", "contents": "On the mechanism of the antimycobacterial activity of isoniazid + prothionamide + dapsone (Isoprodian). Increased antimycobacterial activity of Isoprodian (isoniazid + prothionamide + dapsone) may be due to (i) decreased mutation rate for INH resistance provoked in mycobacteria by DDS (in 2 of 3 strains tested); (ii) leakage of K+, Na+ or Ca++ caused by INH and/or by PTH (in all 3 strains tested), and (iii) indicating damages which may increase the low level penetration of DDS into the cell in sub-MIC concentrations as shown by phage proliferation changes (tested in 1 strain with 1 phage)."}
{"id": "PMID:477454", "title": "Cefazolin treatment of bacterial infections. In vitro and in vivo evaluation.", "content": "Cefazolin sodium was used to treat 20 serious bacterial infections in 19 patients, 11 of whom were infected by coagulase-positive Staphylococci. Clinical and bacteriologic success were initially obtained in 15 patients with 16 infections. 2 additional patients were cured after surgery for removal of foreign bodies and a second course of the antibiotic. 1 asplenic patient had bacteremia and died 5 days after initiating therapy with negative blood cultures and multiple abscesses. 1 patient with endocarditis relapsed 19 months after treatment with 6 weeks of cefazolin and prolonged oral antibiotics. Calculated median, trough, free drug levels were 1.5--15.3 times the minimum inhibitory concentration for 27 of 29 pathogens.", "contents": "Cefazolin treatment of bacterial infections. In vitro and in vivo evaluation. Cefazolin sodium was used to treat 20 serious bacterial infections in 19 patients, 11 of whom were infected by coagulase-positive Staphylococci. Clinical and bacteriologic success were initially obtained in 15 patients with 16 infections. 2 additional patients were cured after surgery for removal of foreign bodies and a second course of the antibiotic. 1 asplenic patient had bacteremia and died 5 days after initiating therapy with negative blood cultures and multiple abscesses. 1 patient with endocarditis relapsed 19 months after treatment with 6 weeks of cefazolin and prolonged oral antibiotics. Calculated median, trough, free drug levels were 1.5--15.3 times the minimum inhibitory concentration for 27 of 29 pathogens."}
{"id": "PMID:477460", "title": "What can be expected from the surgical treatment of craniopharyngiomas in children. Report of 92 cases.", "content": "92 cases of craniopharyngiomas have been operated, 63 with a follow-up extending from 30 months to 20 years. The possibilities of the surgical treatment are mainly discussed on the basis of the anatomical location of the tumors. Simple intrasellar craniopharyngiomas can be operated by a transphenoidal approach with, sometimes, spectacular improvement (case of spontaneous normal pregnancy). The tumors most frequently seen are those with predominantly prechiasmatic expansion. A subfrontal approach is always required, aiming at radical excision. Microscopy can offer interesting possibilities for selective surgery. However, if the vital prognosis is excellent and recurrences rare, functional results remain very poor. In retrochiasmatic tumors involving the posterior hypothalamus, the principle of radical excision must be seriously discussed and sometimes challenged. The various combined approaches are described with their own limits and risks. Other therapeutic techniques are suggested. In many cases, selective neurosurgical dissection may raise some hope for better improvement of functional results.", "contents": "What can be expected from the surgical treatment of craniopharyngiomas in children. Report of 92 cases. 92 cases of craniopharyngiomas have been operated, 63 with a follow-up extending from 30 months to 20 years. The possibilities of the surgical treatment are mainly discussed on the basis of the anatomical location of the tumors. Simple intrasellar craniopharyngiomas can be operated by a transphenoidal approach with, sometimes, spectacular improvement (case of spontaneous normal pregnancy). The tumors most frequently seen are those with predominantly prechiasmatic expansion. A subfrontal approach is always required, aiming at radical excision. Microscopy can offer interesting possibilities for selective surgery. However, if the vital prognosis is excellent and recurrences rare, functional results remain very poor. In retrochiasmatic tumors involving the posterior hypothalamus, the principle of radical excision must be seriously discussed and sometimes challenged. The various combined approaches are described with their own limits and risks. Other therapeutic techniques are suggested. In many cases, selective neurosurgical dissection may raise some hope for better improvement of functional results."}
{"id": "PMID:477461", "title": "Optic glioma in children. Review of 16 cases given megavoltage radiation therapy.", "content": "From 1967 to 1975, 16 consecutive children with optic-tract glioma were treated with postoperative irradiation. Clinical, surgical and pathologic findings have been summarized. Doses ranged from 4,000 to 6,000 rad. As of 1976, follow-up had been obtained in 13 of 16 children. 9 of 13 survived and 3 were lost to follow-up. We have been unable to relate survival to age, sex, pathologic grade, radiation dose of field size. Although all patients in our series were irradiated, it appears that survival is excellent for those with gliomas that are confined to the optic nerve or chiasm without hydrocephalus even if postoperative irradiation is not given. In patients with extension beyond the chiasm or with hydrocephalus, survival may be improved by the addition of irradiation. A staging system is proposed.", "contents": "Optic glioma in children. Review of 16 cases given megavoltage radiation therapy. From 1967 to 1975, 16 consecutive children with optic-tract glioma were treated with postoperative irradiation. Clinical, surgical and pathologic findings have been summarized. Doses ranged from 4,000 to 6,000 rad. As of 1976, follow-up had been obtained in 13 of 16 children. 9 of 13 survived and 3 were lost to follow-up. We have been unable to relate survival to age, sex, pathologic grade, radiation dose of field size. Although all patients in our series were irradiated, it appears that survival is excellent for those with gliomas that are confined to the optic nerve or chiasm without hydrocephalus even if postoperative irradiation is not given. In patients with extension beyond the chiasm or with hydrocephalus, survival may be improved by the addition of irradiation. A staging system is proposed."}
{"id": "PMID:477455", "title": "Adoption of Korean children by New York area couples: a preliminary study.", "content": "This paper reports on the first segment of a longitudinal study of the effects of transracial and transcultural transplantation into American families.", "contents": "Adoption of Korean children by New York area couples: a preliminary study. This paper reports on the first segment of a longitudinal study of the effects of transracial and transcultural transplantation into American families."}
{"id": "PMID:477462", "title": "Childhood moyamoya syndrome. Postradiation pathogenesis.", "content": "The development of typical clinical and radiological features of moyamoya syndrome is described in 3 children following radiation therapy for a gliomatous tumor at the base of the brain. The effect of radiation therapy on the medium-sized arteries is discussed with a review of the literature. Radiation therapy as the causative factor for the narrowing or occlusion of the carotid siphon is discussed.", "contents": "Childhood moyamoya syndrome. Postradiation pathogenesis. The development of typical clinical and radiological features of moyamoya syndrome is described in 3 children following radiation therapy for a gliomatous tumor at the base of the brain. The effect of radiation therapy on the medium-sized arteries is discussed with a review of the literature. Radiation therapy as the causative factor for the narrowing or occlusion of the carotid siphon is discussed."}
{"id": "PMID:477463", "title": "Lipoma of the corpus callosum: report of a case and review of the literature.", "content": "A case of lipoma of the corpus callosum associated with subcutaneous lipoma, whose symptoms were headache and trembling of the extremities, was reported. Roentgenograms showed characteristic findings and computerized tomography revealed a mass of very low density. The literature was reviewed and 15 cases not included in previous compilations were found. Clinical and radiological findings in 89 cases including the present case were analyzed.", "contents": "Lipoma of the corpus callosum: report of a case and review of the literature. A case of lipoma of the corpus callosum associated with subcutaneous lipoma, whose symptoms were headache and trembling of the extremities, was reported. Roentgenograms showed characteristic findings and computerized tomography revealed a mass of very low density. The literature was reviewed and 15 cases not included in previous compilations were found. Clinical and radiological findings in 89 cases including the present case were analyzed."}
{"id": "PMID:477464", "title": "Comparison of the effects of surgical decompression and resection of local edema in the therapy of experimental brain trauma. Investigation of ICP, EEG and cerebral metabolism in cats.", "content": "The effect of decompressive trepanation was compared to that of surgical resection of the traumatized tissue in the course of traumatic brain edema in standardized experimental brain trauma. Following a right parietal cold injury, the following parameters were monitored continuously in 12 cats: ventricular pressure, epidural pressure over both hemispheres, arterial and central venous pressure and heart rate. The EEG was evaluated continuously, using a computer (power spectra). After catheterization of the superior sagittal sinus, cerebral arteriovenous differences of glucose, lactate, O2 and CO2 were calculated. 6 animals were treated surgically when showing elevated intracranial pressure ICP and markedly altered EEG. In 3 animals, the right hemisphere was decompressed by extensive resection of bone and dura. In 3 further animals, the softened brain tissue of the cold lesion was resected and the skull defect closed. 6 untreated animals were used in controls. A decompression by skull hemiresection for ablation of the injured cortex abolished the high intracranial pressure, but only the latter method seemed to prevent further damage. This could be demonstrated by the EEG registration, and by the normalization of arteriovenous metabolite differences. Only animals treated with edema resection had a normal arousal reaction and survived the trauma. The results indicate, that only an ablation of the local injury will prevent further damage to the brain. After decompressive trepanation alone, the progression of tissue edema is not interrupted. As can be seen from the literature, the poor results obtained even from extensive decompressive operations in traumatic brain edema, indicate that the further development of edema is independent of the intracranial pressure, whereas the favorable results of resection of lobar contusions show an interruption of the spread of dysbolism.", "contents": "Comparison of the effects of surgical decompression and resection of local edema in the therapy of experimental brain trauma. Investigation of ICP, EEG and cerebral metabolism in cats. The effect of decompressive trepanation was compared to that of surgical resection of the traumatized tissue in the course of traumatic brain edema in standardized experimental brain trauma. Following a right parietal cold injury, the following parameters were monitored continuously in 12 cats: ventricular pressure, epidural pressure over both hemispheres, arterial and central venous pressure and heart rate. The EEG was evaluated continuously, using a computer (power spectra). After catheterization of the superior sagittal sinus, cerebral arteriovenous differences of glucose, lactate, O2 and CO2 were calculated. 6 animals were treated surgically when showing elevated intracranial pressure ICP and markedly altered EEG. In 3 animals, the right hemisphere was decompressed by extensive resection of bone and dura. In 3 further animals, the softened brain tissue of the cold lesion was resected and the skull defect closed. 6 untreated animals were used in controls. A decompression by skull hemiresection for ablation of the injured cortex abolished the high intracranial pressure, but only the latter method seemed to prevent further damage. This could be demonstrated by the EEG registration, and by the normalization of arteriovenous metabolite differences. Only animals treated with edema resection had a normal arousal reaction and survived the trauma. The results indicate, that only an ablation of the local injury will prevent further damage to the brain. After decompressive trepanation alone, the progression of tissue edema is not interrupted. As can be seen from the literature, the poor results obtained even from extensive decompressive operations in traumatic brain edema, indicate that the further development of edema is independent of the intracranial pressure, whereas the favorable results of resection of lobar contusions show an interruption of the spread of dysbolism."}
{"id": "PMID:477456", "title": "A model for systematic child protective service training.", "content": "A model training program structured to provide basic knowledge and skills for child protective workers appears to be meeting its objectives.", "contents": "A model for systematic child protective service training. A model training program structured to provide basic knowledge and skills for child protective workers appears to be meeting its objectives."}
{"id": "PMID:477468", "title": "[Topography and surgico-tactical principles of the mesocaval shunt].", "content": "The mesocaval shunt has special advantages. It can be applied also in patient with worse condition (Child B and C). The technically simple performance according to the original method of Drapanas is recommended provided that anatomical variants at the surgical trunk of the vena mesenterica superior are known. The anatomical situation should be investigated preoperatively by direct (umbilical vein catheter) or indirect mesentericography. The access via umbilical vein offers in addition the possibility of coronary vein embolisation in emergency patients.", "contents": "[Topography and surgico-tactical principles of the mesocaval shunt]. The mesocaval shunt has special advantages. It can be applied also in patient with worse condition (Child B and C). The technically simple performance according to the original method of Drapanas is recommended provided that anatomical variants at the surgical trunk of the vena mesenterica superior are known. The anatomical situation should be investigated preoperatively by direct (umbilical vein catheter) or indirect mesentericography. The access via umbilical vein offers in addition the possibility of coronary vein embolisation in emergency patients."}
{"id": "PMID:477457", "title": "Clinical illustrations of the sexual misuse of girls.", "content": "The sexual misuse of girls, usually incestuous, often results in profound psychological impairment, sometimes transmitted to the next generation.", "contents": "Clinical illustrations of the sexual misuse of girls. The sexual misuse of girls, usually incestuous, often results in profound psychological impairment, sometimes transmitted to the next generation."}
{"id": "PMID:477469", "title": "[Prevention of postoperative pancreatic complications following duodenopancreatectomy using somatostatin].", "content": "Somatostatin was prophylactically administered to 10 patients who had surgery for pancreatic diseases. The postoperative course uncomplicated with no increase in serum amylase levels. Bile induced acute pancreatitis in six beagles was prophylactically treated with somatostatin. The results demonstrate that SRIF not only inhibits basal but also pancreatic-induced blood amylase and enzyme activities in the dog. SRIF-treated animals were in good general condition compared with untreated controls, macroscopic and histologic aspects of the pancreas were significantly improved.", "contents": "[Prevention of postoperative pancreatic complications following duodenopancreatectomy using somatostatin]. Somatostatin was prophylactically administered to 10 patients who had surgery for pancreatic diseases. The postoperative course uncomplicated with no increase in serum amylase levels. Bile induced acute pancreatitis in six beagles was prophylactically treated with somatostatin. The results demonstrate that SRIF not only inhibits basal but also pancreatic-induced blood amylase and enzyme activities in the dog. SRIF-treated animals were in good general condition compared with untreated controls, macroscopic and histologic aspects of the pancreas were significantly improved."}
{"id": "PMID:477471", "title": "[Clinical and morphological properties of cold nodules formed in the thyroid gland of patients treated with I-131 for hyperthyreosis].", "content": "The authors report on the clinical and morphological features of patients who had been previously treated for toxic adenoma with different doses of 131I isotope, and were then operated on for cold nodule. Progressive pseudoatypic and H\u00fcrthle-cell transformations, which are difficult to distinguish from different-type thyroid carcinomas, were revealed in one and a solid anaplastic carcinoma in another of the five patients during histological examination of operative specimens in serial sections. On the basis of these findings the authors support the function-protective operative treatment of single toxic adenomas, although the causal relationship between isotope treatment and consecutive carcinoma can not be verified unanimously.", "contents": "[Clinical and morphological properties of cold nodules formed in the thyroid gland of patients treated with I-131 for hyperthyreosis]. The authors report on the clinical and morphological features of patients who had been previously treated for toxic adenoma with different doses of 131I isotope, and were then operated on for cold nodule. Progressive pseudoatypic and H\u00fcrthle-cell transformations, which are difficult to distinguish from different-type thyroid carcinomas, were revealed in one and a solid anaplastic carcinoma in another of the five patients during histological examination of operative specimens in serial sections. On the basis of these findings the authors support the function-protective operative treatment of single toxic adenomas, although the causal relationship between isotope treatment and consecutive carcinoma can not be verified unanimously."}
{"id": "PMID:477470", "title": "[Studies on the effect of 4-methyl-umbelliferon (Hymecromone) in patients following surgical revision of the biliary pathways].", "content": "The effect of 4-methylumbelliferone (hymecromone) on postoperative gall volume and residual pressure, serum enzymes, and other parameters in 50 patients after cholecystectomy and choledochotomy with T-tube drainage is measured by a clinical double-blind study. There were several interesting findings: (a) significant less gall flowing from the T tube, (b) highly significant less activity of the enzymes basic phosphatase and of the gamma-glutamyltranspeptidase, and (c) fewer need of analgesics.", "contents": "[Studies on the effect of 4-methyl-umbelliferon (Hymecromone) in patients following surgical revision of the biliary pathways]. The effect of 4-methylumbelliferone (hymecromone) on postoperative gall volume and residual pressure, serum enzymes, and other parameters in 50 patients after cholecystectomy and choledochotomy with T-tube drainage is measured by a clinical double-blind study. There were several interesting findings: (a) significant less gall flowing from the T tube, (b) highly significant less activity of the enzymes basic phosphatase and of the gamma-glutamyltranspeptidase, and (c) fewer need of analgesics."}
{"id": "PMID:477458", "title": "Preventing worker burnout in child welfare.", "content": "Awareness is growing that worker burnout is a serious problem in the child welfare field. This paper examines the multiple causes of the phenomenon, and suggests approaches to prevention.", "contents": "Preventing worker burnout in child welfare. Awareness is growing that worker burnout is a serious problem in the child welfare field. This paper examines the multiple causes of the phenomenon, and suggests approaches to prevention."}
{"id": "PMID:477472", "title": "[Man in old age: from the viewpoint of the surgeon].", "content": "The most important problems of surgery in the aged patient are: immediate recognition of risk factor (few, but reliable laboratory parameters in the sense of \"searching tactics\": status of blood, blood sugar, urea-N, creatinine, cholinesterase), careful procedural choice, immediate mobilisation postoperatively, subsequent rehabilitation, if necessary. The operation itself must be fast but careful and as restricted as possible.", "contents": "[Man in old age: from the viewpoint of the surgeon]. The most important problems of surgery in the aged patient are: immediate recognition of risk factor (few, but reliable laboratory parameters in the sense of \"searching tactics\": status of blood, blood sugar, urea-N, creatinine, cholinesterase), careful procedural choice, immediate mobilisation postoperatively, subsequent rehabilitation, if necessary. The operation itself must be fast but careful and as restricted as possible."}
{"id": "PMID:477475", "title": "Cellular density of normal corneal endothelium.", "content": "With the aid of the specular microscope photographs were made of the endothelium of 72 subjects, 41 men and 31 women, aged from 24 to 86 years. The number of endothelial cells was counted by projecting the photographs and counting the cells in a given area. The average variation per eye was 4.9% with a standard deviation of 3.7. A difference in magnification associated with differences in the thickness of the cornea was not found. There was a significant negative correlation (Pearson) between age and cell density: correlation coefficient 0.395, significance P = 0.001. Regression analysis of this correlation gave the following equation: CL = 3081 - 8.8 L, where CL = cell density at age L. The error is 97 cells more or less than this figure.", "contents": "Cellular density of normal corneal endothelium. With the aid of the specular microscope photographs were made of the endothelium of 72 subjects, 41 men and 31 women, aged from 24 to 86 years. The number of endothelial cells was counted by projecting the photographs and counting the cells in a given area. The average variation per eye was 4.9% with a standard deviation of 3.7. A difference in magnification associated with differences in the thickness of the cornea was not found. There was a significant negative correlation (Pearson) between age and cell density: correlation coefficient 0.395, significance P = 0.001. Regression analysis of this correlation gave the following equation: CL = 3081 - 8.8 L, where CL = cell density at age L. The error is 97 cells more or less than this figure."}
{"id": "PMID:477476", "title": "Iatrogenic endothelial damage during cataract extraction.", "content": "Morphological changes in the endothelium of rabbits' corneas after rinsing with Ringer's solution, chymytrase 0.05% or acetylcholine could not be demonstrated by vital staining with nitroblue tetrazolium. Local lesions caused by manipulations in the anterior chamber were conspicuous. Endothelial lesions due to manipulations with an intra-ocular lens were studied in the same way.", "contents": "Iatrogenic endothelial damage during cataract extraction. Morphological changes in the endothelium of rabbits' corneas after rinsing with Ringer's solution, chymytrase 0.05% or acetylcholine could not be demonstrated by vital staining with nitroblue tetrazolium. Local lesions caused by manipulations in the anterior chamber were conspicuous. Endothelial lesions due to manipulations with an intra-ocular lens were studied in the same way."}
{"id": "PMID:477477", "title": "Continuous lavage in (chemical) corneal lesions.", "content": "In a small number of patients with severe chemical corneal damage and in a few patients with corneal ulcers, continuous lavage of the conjunctival sac with a mixture of the patient's own serum, tissue culture fluid and various medicaments was carried out with great success.", "contents": "Continuous lavage in (chemical) corneal lesions. In a small number of patients with severe chemical corneal damage and in a few patients with corneal ulcers, continuous lavage of the conjunctival sac with a mixture of the patient's own serum, tissue culture fluid and various medicaments was carried out with great success."}
{"id": "PMID:477478", "title": "Pathologico-optic approach to cataract and lens.", "content": "From the weight of the lenses of patients with senile cataract, ultrasonic measurements of the eyes of patients with acute diabetic refractive changes, and calculations on the basis of Legrand's theoretical eye, it appears that the conclusion can be drawn that these refractive changes are due to alterations in the refractive indices of the lens.", "contents": "Pathologico-optic approach to cataract and lens. From the weight of the lenses of patients with senile cataract, ultrasonic measurements of the eyes of patients with acute diabetic refractive changes, and calculations on the basis of Legrand's theoretical eye, it appears that the conclusion can be drawn that these refractive changes are due to alterations in the refractive indices of the lens."}
{"id": "PMID:477479", "title": "Lens injury in children treated with irido-capsula supported intra-ocular lenses.", "content": "The treatment of lens injury in children with irido-capsula supported intraocular lenses is described. A consecutive series of 73 children is analyzed as to the functional results and complications. Surgery and anaesthesia are discussed in detail. Guidelines are given for future straightforward management of eye injury in children, using this promising possibility of visual rehabilitation.", "contents": "Lens injury in children treated with irido-capsula supported intra-ocular lenses. The treatment of lens injury in children with irido-capsula supported intraocular lenses is described. A consecutive series of 73 children is analyzed as to the functional results and complications. Surgery and anaesthesia are discussed in detail. Guidelines are given for future straightforward management of eye injury in children, using this promising possibility of visual rehabilitation."}
{"id": "PMID:477483", "title": "The superior oblique: a challenging extraocular muscle.", "content": "Isolated paralysis of one or both superior oblique muscles in a consecutive series of 43 patients was found to occur most often by indirect injury to the skull. The etiology in the group of spontaneous cases was mostly vascular. With rare exceptions the latter recovered spontaneously. Those with traumatic palsy may be due to \"contrecoup\" trauma at the decussation of the nerves or by decompensation of the cerebral fusional mechanism, with dissociation of the images of the two eyes and subsequent vertical deviation. The traumatic cases never recover spontaneously and recession or myectomy of the ipsilateral inferior oblique with or without recession of the contralateral inferior rectus is recommended but only in cases of more than 8 diopters squint, and in cases which do not adapt themselves by minor head-tilting.", "contents": "The superior oblique: a challenging extraocular muscle. Isolated paralysis of one or both superior oblique muscles in a consecutive series of 43 patients was found to occur most often by indirect injury to the skull. The etiology in the group of spontaneous cases was mostly vascular. With rare exceptions the latter recovered spontaneously. Those with traumatic palsy may be due to \"contrecoup\" trauma at the decussation of the nerves or by decompensation of the cerebral fusional mechanism, with dissociation of the images of the two eyes and subsequent vertical deviation. The traumatic cases never recover spontaneously and recession or myectomy of the ipsilateral inferior oblique with or without recession of the contralateral inferior rectus is recommended but only in cases of more than 8 diopters squint, and in cases which do not adapt themselves by minor head-tilting."}
{"id": "PMID:477484", "title": "The influence of clonidine on intraocular pressure.", "content": "In order to study the influence of clonidine on intraocular pressure (IOP), the drug was administered via different routes. The experiments were performed on chloralose-anaesthetized cats. The rise in IOP observed after administration of clonidine into the external carotid arteries was subjected to a pharmacological analysis. Our experiments suggest that the increase in IOP is due to the contraction of eyelids and extraocular muscles, mediated by the influence of clonidine on alpha-adrenoceptors. The IOP-lowering effect of clonidine was more pronounced after its infusion into the left vertebral artery than after its intravenous injection. This difference would suggest that the clonidine-induced decrease in IOP may be mediated via a central mechanism.", "contents": "The influence of clonidine on intraocular pressure. In order to study the influence of clonidine on intraocular pressure (IOP), the drug was administered via different routes. The experiments were performed on chloralose-anaesthetized cats. The rise in IOP observed after administration of clonidine into the external carotid arteries was subjected to a pharmacological analysis. Our experiments suggest that the increase in IOP is due to the contraction of eyelids and extraocular muscles, mediated by the influence of clonidine on alpha-adrenoceptors. The IOP-lowering effect of clonidine was more pronounced after its infusion into the left vertebral artery than after its intravenous injection. This difference would suggest that the clonidine-induced decrease in IOP may be mediated via a central mechanism."}
{"id": "PMID:477485", "title": "Tobacco amblyopia: the effect of treatment on the electroretinogram.", "content": "In seven recently diagnosed and untreated patients with tobacco amblyopia the light and dark adapted electroretionograms were recorded together with visual acuity, central fields and the Farnsworth-Munsell 100-Hue test. After 3 months treatment with injections of hydroxocobalamin all these tests were repeated. An improvement in visual acuity resulted in all cases. A significant reduction in the Farnsworth-Munsell total error score of the seven patients was noted (p less than 0.01). A significant increase in the amplitude of the light adapted b-wave of the ERG was found (0.01 less than p less than 0.02) following the course of hydroxocobalamin injections. No other significant changes in the ERG were demonstrated. It is suggested that the ERG changes demonstrated an improvement in an abnormal biochemical state in those bipolar and perhaps M\u00fcller cell of the inner nuclear layer of the retina which make electrophysiological connnections chiefly with foveal cones.", "contents": "Tobacco amblyopia: the effect of treatment on the electroretinogram. In seven recently diagnosed and untreated patients with tobacco amblyopia the light and dark adapted electroretionograms were recorded together with visual acuity, central fields and the Farnsworth-Munsell 100-Hue test. After 3 months treatment with injections of hydroxocobalamin all these tests were repeated. An improvement in visual acuity resulted in all cases. A significant reduction in the Farnsworth-Munsell total error score of the seven patients was noted (p less than 0.01). A significant increase in the amplitude of the light adapted b-wave of the ERG was found (0.01 less than p less than 0.02) following the course of hydroxocobalamin injections. No other significant changes in the ERG were demonstrated. It is suggested that the ERG changes demonstrated an improvement in an abnormal biochemical state in those bipolar and perhaps M\u00fcller cell of the inner nuclear layer of the retina which make electrophysiological connnections chiefly with foveal cones."}
{"id": "PMID:477489", "title": "S.I. units in ophthalmological practice.", "content": "The introduction of S.I. units internationally means that an adaptation of the use of certain units in ophthalmological practice will have to be considered. This has important consequences, especially for the measurement of intra-ocular pressure. A survey is given and some suggestions are made for the practical use of an adapted unit for intra-ocular pressure measurement.", "contents": "S.I. units in ophthalmological practice. The introduction of S.I. units internationally means that an adaptation of the use of certain units in ophthalmological practice will have to be considered. This has important consequences, especially for the measurement of intra-ocular pressure. A survey is given and some suggestions are made for the practical use of an adapted unit for intra-ocular pressure measurement."}
{"id": "PMID:477488", "title": "TV pattern evoked cortical responses without and with film mixing.", "content": "To obtain measurable TV pattern evoked cortical responses of children, the alpha-rhythm of the EEG must be suppressed. In our study, mixing pattern stimuli and film proved to be an effective way of doing this.", "contents": "TV pattern evoked cortical responses without and with film mixing. To obtain measurable TV pattern evoked cortical responses of children, the alpha-rhythm of the EEG must be suppressed. In our study, mixing pattern stimuli and film proved to be an effective way of doing this."}
{"id": "PMID:477520", "title": "[Diagnosis of leucocytes in urine by means of a test-strip (author's transl)].", "content": "A test-strip for diagnosing urinary leucocytes (Cytur) was evaluated by 11 centres, on a total of 1985 voided urine specimens. Results were compared with chamber counting of uncentrifuged urine, as well as by microscopic examination of spun sediment. With a reaction time of 15 minutes the test-strip compared well with the other two methods. When urine is allowed to stand, the lysis of leucocytes exaggerates the colour reaction of the test-strip. Red blood cells, bacteria, sperm and nitrite in urine do not influence the results, except Trichomonas which increases the reaction. Ascorbic acid and albumin slightly inhibit the reaction.", "contents": "[Diagnosis of leucocytes in urine by means of a test-strip (author's transl)]. A test-strip for diagnosing urinary leucocytes (Cytur) was evaluated by 11 centres, on a total of 1985 voided urine specimens. Results were compared with chamber counting of uncentrifuged urine, as well as by microscopic examination of spun sediment. With a reaction time of 15 minutes the test-strip compared well with the other two methods. When urine is allowed to stand, the lysis of leucocytes exaggerates the colour reaction of the test-strip. Red blood cells, bacteria, sperm and nitrite in urine do not influence the results, except Trichomonas which increases the reaction. Ascorbic acid and albumin slightly inhibit the reaction."}
{"id": "PMID:477521", "title": "[Fibrinolytic treatment with urokinase of post acute phlebothromboses (author's transl)].", "content": "Standardised urokinase-heparin treatment was administered to 32 patients one to six weeks after deep-vein thrombosis. Treatment generally continued over 7-14 days. Complete recanalisation occurred in six, partial in 18 patients. In eight patients there was no evidence of thrombolysis in the phlebogram.", "contents": "[Fibrinolytic treatment with urokinase of post acute phlebothromboses (author's transl)]. Standardised urokinase-heparin treatment was administered to 32 patients one to six weeks after deep-vein thrombosis. Treatment generally continued over 7-14 days. Complete recanalisation occurred in six, partial in 18 patients. In eight patients there was no evidence of thrombolysis in the phlebogram."}
{"id": "PMID:477536", "title": "[\"Plica disease\" (synovial folds) of the knee-joint: arthroscopic and histological findings, with suggestions for treatment (author's transl)].", "content": "A mediopatellar plica (synovial fold) of the knee-joint may develop without recognisable cause in adolescents or young adults, predominantly females. It leads to pain on pressure over the medial knee compartment, sudden or \"springing\" intraarticular movements and pseudolocking of the joint. Similar plicae occur after traumatic joint contusion, with meniscus disease, or more rarely with arthrosis deformans. Histologically they are characterized by band-like fibrosed evaginations of the synovial membrane and of the synovial fat and connective tissue into the joint spaces. The following therapeutic suggestions, based on the personal experience of 15 cases, are made in the knowledge that significant inflammatory or proliferative arthritic changes can be excluded: the plica can be cut through under arthroscopy; chondromalacial defects, directly or indirectly caused by plical rubbing, of the medial femoral condyle and the medial patella can be removed, also under arthroscopic control, with an electric razor. Arthrotomy is no longer needed in most cases.", "contents": "[\"Plica disease\" (synovial folds) of the knee-joint: arthroscopic and histological findings, with suggestions for treatment (author's transl)]. A mediopatellar plica (synovial fold) of the knee-joint may develop without recognisable cause in adolescents or young adults, predominantly females. It leads to pain on pressure over the medial knee compartment, sudden or \"springing\" intraarticular movements and pseudolocking of the joint. Similar plicae occur after traumatic joint contusion, with meniscus disease, or more rarely with arthrosis deformans. Histologically they are characterized by band-like fibrosed evaginations of the synovial membrane and of the synovial fat and connective tissue into the joint spaces. The following therapeutic suggestions, based on the personal experience of 15 cases, are made in the knowledge that significant inflammatory or proliferative arthritic changes can be excluded: the plica can be cut through under arthroscopy; chondromalacial defects, directly or indirectly caused by plical rubbing, of the medial femoral condyle and the medial patella can be removed, also under arthroscopic control, with an electric razor. Arthrotomy is no longer needed in most cases."}
{"id": "PMID:477537", "title": "[Danazol treatment of hereditary angioneurotic oedema (author's transl)].", "content": "Danazol, an attenuated androgen, was administered to four patients with hereditary angioneurotic oedema, with rapid and complete response without side-effects. The follow-up period has now been up to 17 months. In all patients there was an indirect indication that their hormonal state influenced the course of the disease.", "contents": "[Danazol treatment of hereditary angioneurotic oedema (author's transl)]. Danazol, an attenuated androgen, was administered to four patients with hereditary angioneurotic oedema, with rapid and complete response without side-effects. The follow-up period has now been up to 17 months. In all patients there was an indirect indication that their hormonal state influenced the course of the disease."}
{"id": "PMID:477549", "title": "[Local recurrences of carcinoma of the breast (author's transl)].", "content": "Distant metastases were sought for in 90 patients with local recurrence of breast carcinoma. Only 11 patients had demonstrable spread of the disease at the time of referral. Two-thirds of all local recurrences presented within two years of the primary operation. In 66 out of 90 patients the recurrence was in the chest wall, in 11 cases it was in the chest wall and in the lymphatic system and in only 13 cases was it primarily in the regional lymph nodes. The recurrence rate depended mainly on the previous treatment of the primary tumour: 42 recurrences were found in those cases treated only by surgery, 46 in cases treated surgically and with low-dose post-operative irradiation and only two in patients treated with over 4500 rad to the area of subsequent recurrence.", "contents": "[Local recurrences of carcinoma of the breast (author's transl)]. Distant metastases were sought for in 90 patients with local recurrence of breast carcinoma. Only 11 patients had demonstrable spread of the disease at the time of referral. Two-thirds of all local recurrences presented within two years of the primary operation. In 66 out of 90 patients the recurrence was in the chest wall, in 11 cases it was in the chest wall and in the lymphatic system and in only 13 cases was it primarily in the regional lymph nodes. The recurrence rate depended mainly on the previous treatment of the primary tumour: 42 recurrences were found in those cases treated only by surgery, 46 in cases treated surgically and with low-dose post-operative irradiation and only two in patients treated with over 4500 rad to the area of subsequent recurrence."}
{"id": "PMID:477550", "title": "[Bronchodilator effect of methylxanthine derivatives (author's transl)].", "content": "The bronchodilator effect of theophylline ethylenediamine was compared with that of a combination of the methylxanthines theophylline, diprophylline and proxyphylline, after administration of slow-release capsules. At an initial dose of twice two capsules the two drug preparations produced similar, therapeutically significant, effects. When reducing the dose to twice one capsule, during long-term treatment, there was a clinically and statistically significantly greater bronchodilator effect among those patients receiving the combined methylxanthine preparation.", "contents": "[Bronchodilator effect of methylxanthine derivatives (author's transl)]. The bronchodilator effect of theophylline ethylenediamine was compared with that of a combination of the methylxanthines theophylline, diprophylline and proxyphylline, after administration of slow-release capsules. At an initial dose of twice two capsules the two drug preparations produced similar, therapeutically significant, effects. When reducing the dose to twice one capsule, during long-term treatment, there was a clinically and statistically significantly greater bronchodilator effect among those patients receiving the combined methylxanthine preparation."}
{"id": "PMID:477551", "title": "[Interaction of rifampicin with phenprocoumon (author's transl)].", "content": "Prothrombin time and plasma phenprocoumon levels were serially controlled, at short intervals, in three tubercular patients receiving both tuberculostatic drugs and phenprocoumon. The required phenprocoumon dose was continuously adapted to the prothrombin time values. During and after treatment with rifampicin optimal levels of prothrombin time were difficult or even impossible to achieve in two of the three patients. This confirms the known loss of effectiveness of the coumarins induced by rifampicin. This action has a slower onset and disappears more gradually than after acenocoumarol or warfarin. It is, therefore, likely that these two latter drugs are more suitable in patients treated with rifampicin than phenprocoumon.", "contents": "[Interaction of rifampicin with phenprocoumon (author's transl)]. Prothrombin time and plasma phenprocoumon levels were serially controlled, at short intervals, in three tubercular patients receiving both tuberculostatic drugs and phenprocoumon. The required phenprocoumon dose was continuously adapted to the prothrombin time values. During and after treatment with rifampicin optimal levels of prothrombin time were difficult or even impossible to achieve in two of the three patients. This confirms the known loss of effectiveness of the coumarins induced by rifampicin. This action has a slower onset and disappears more gradually than after acenocoumarol or warfarin. It is, therefore, likely that these two latter drugs are more suitable in patients treated with rifampicin than phenprocoumon."}
{"id": "PMID:477559", "title": "[The effectiveness of cervical cytology screening for cervical cancer (author's transl)].", "content": "Cervical cytology screening was performed, between July 1971 and the end of 1976, on 503,870 specimens from 226,428 women. During this period the rate of positive or suspicious findings fell from 2.6% to 0.9%. The number of specimens judged suspicious for invasive carcinoma, carcinoma in situ or with severe dysplasia (among those women who had undergone serial examinations) was 5 per thousand for the first specimen, 2 per thousand for a subsequent second specimen, and 1.6 per thousand for a subsequent third specimen. The screening programme detected (later confirmed histologically) 272 cases of invasive carcinoma and 948 of carcinoma in situ or severe dysplasia. Related to the number of women screended at various ages, the frequency of carcinoma in situ and severe dysplasia had a peak between the 25th and 29th year of life, with a second, lower peak at around the 65th year. For invasive cervical carcinoma the frequency remained high into old age. The proportion of histologically proven cases of invasive carcinoma fell continuously from 1963/66, at 3.6 per thousand, until 1976, at 1.6 per thousand.", "contents": "[The effectiveness of cervical cytology screening for cervical cancer (author's transl)]. Cervical cytology screening was performed, between July 1971 and the end of 1976, on 503,870 specimens from 226,428 women. During this period the rate of positive or suspicious findings fell from 2.6% to 0.9%. The number of specimens judged suspicious for invasive carcinoma, carcinoma in situ or with severe dysplasia (among those women who had undergone serial examinations) was 5 per thousand for the first specimen, 2 per thousand for a subsequent second specimen, and 1.6 per thousand for a subsequent third specimen. The screening programme detected (later confirmed histologically) 272 cases of invasive carcinoma and 948 of carcinoma in situ or severe dysplasia. Related to the number of women screended at various ages, the frequency of carcinoma in situ and severe dysplasia had a peak between the 25th and 29th year of life, with a second, lower peak at around the 65th year. For invasive cervical carcinoma the frequency remained high into old age. The proportion of histologically proven cases of invasive carcinoma fell continuously from 1963/66, at 3.6 per thousand, until 1976, at 1.6 per thousand."}
{"id": "PMID:477560", "title": "[Does low-dosage heparin treatment require serial haematological controls? (author's transl)].", "content": "Blanket serial controls are not necessary in low-dosage heparin treatment. It would, in any case, be difficult under normal clinical conditions and would run counter to the whole conception of low-dose heparin treatment. However, in problem cases with an increased thrombo-embolic risk, sensitive methods for monitoring the heparin effect are recommended. A study on 150 patients has indicated that the most sensitive method is the use of chromogenic substrates. Thrombin time, using low-concentration thrombin solution of 1.5 NIH units/ml, thrombelastogram and activated partial thromboplastin time are less sensitive. Antithrombin III levels should be determined in all cases of increased heparin tolerance. With reduced antithrombin III levels and higher body weight an increase of the standard dose from 5000 U.S.P. units heparin t. i. d. subcutaneously to 7500 U.S.P. units t. i. d. should be considered.", "contents": "[Does low-dosage heparin treatment require serial haematological controls? (author's transl)]. Blanket serial controls are not necessary in low-dosage heparin treatment. It would, in any case, be difficult under normal clinical conditions and would run counter to the whole conception of low-dose heparin treatment. However, in problem cases with an increased thrombo-embolic risk, sensitive methods for monitoring the heparin effect are recommended. A study on 150 patients has indicated that the most sensitive method is the use of chromogenic substrates. Thrombin time, using low-concentration thrombin solution of 1.5 NIH units/ml, thrombelastogram and activated partial thromboplastin time are less sensitive. Antithrombin III levels should be determined in all cases of increased heparin tolerance. With reduced antithrombin III levels and higher body weight an increase of the standard dose from 5000 U.S.P. units heparin t. i. d. subcutaneously to 7500 U.S.P. units t. i. d. should be considered."}
{"id": "PMID:477561", "title": "[The diagnosis of phaeochromocytoma: sensitivity of vanillylmandelic acid and urinary catecholamine determination and the Katecult test (author's transl)].", "content": "Tumour weight was compared with maximal vanillylmandelic acid and catecholamine excretion in 24-hour urine in 21 patients with phaeochromocytoma. The tumour weight correlated both with vanillylmandelic acid (r = 0.805, P less than 0.001) as well as urinary catecholamine levels (r = 0.725, P less than 0.001). Normal vanillylmandelic acid excretion was found in seven patients; urinary catecholamine levels were abnormal in all patients. The Katecult test was additionally performed in ten patients, with nine positive results.", "contents": "[The diagnosis of phaeochromocytoma: sensitivity of vanillylmandelic acid and urinary catecholamine determination and the Katecult test (author's transl)]. Tumour weight was compared with maximal vanillylmandelic acid and catecholamine excretion in 24-hour urine in 21 patients with phaeochromocytoma. The tumour weight correlated both with vanillylmandelic acid (r = 0.805, P less than 0.001) as well as urinary catecholamine levels (r = 0.725, P less than 0.001). Normal vanillylmandelic acid excretion was found in seven patients; urinary catecholamine levels were abnormal in all patients. The Katecult test was additionally performed in ten patients, with nine positive results."}
{"id": "PMID:477562", "title": "[Effect of a 2 mg thyroxine bolus on the hypophyseal-thyroid axis (author's transl)].", "content": "The effect of a single dose of 2 mg L-thyroxine on central and peripheral parameters of the hypophyseal-thyroid axis was studied on ten euthyroid and ten hypothyroid patients, and ten with euthyroid goitre. Without the occurrence of hyperthyroid symptoms, the T4 serum level was raised for five days in the euthyroid group, for 11 days in the hypothyroid ones. During this time the T3 serum level did not alter. Suppression of iodine uptake by the thyroid and TSH secretion of the hypophysis persisted longer, both for basal and TRH-stimulated liberation, than the substitution effect. Even when the T4 bolus has to be at a higher total T4 dose for the same therapeutic effect than with daily small single doses, it is a comparable alternative to present-day forms of treatment. It is of particular advantage where there are medical reasons for widely spaced drug intake or rapid normalisation of the T4 serum level is deemed desirable.", "contents": "[Effect of a 2 mg thyroxine bolus on the hypophyseal-thyroid axis (author's transl)]. The effect of a single dose of 2 mg L-thyroxine on central and peripheral parameters of the hypophyseal-thyroid axis was studied on ten euthyroid and ten hypothyroid patients, and ten with euthyroid goitre. Without the occurrence of hyperthyroid symptoms, the T4 serum level was raised for five days in the euthyroid group, for 11 days in the hypothyroid ones. During this time the T3 serum level did not alter. Suppression of iodine uptake by the thyroid and TSH secretion of the hypophysis persisted longer, both for basal and TRH-stimulated liberation, than the substitution effect. Even when the T4 bolus has to be at a higher total T4 dose for the same therapeutic effect than with daily small single doses, it is a comparable alternative to present-day forms of treatment. It is of particular advantage where there are medical reasons for widely spaced drug intake or rapid normalisation of the T4 serum level is deemed desirable."}
{"id": "PMID:477563", "title": "[Noninvasive screening of cerebral death by Doppler sonography (author's transl)].", "content": "Directional Doppler sonography of the supratrochlear and extracranial cerebral arteries was performed on 16 patients without any cerebral flow, as confirmed angiographically. The findings were inconsistent for the supratrochlear artery, but the internal carotid and vertebral arteries had characteristic changes in the Doppler curve with clearly diminished systolic flow velocity and flow reversal during diastole. These results were never seen in arteriosclerotic occlusion, hypoxaemic or toxic cerebral oedema. Doppler sonography thus helps to formulate indications for early cerebral angiography.", "contents": "[Noninvasive screening of cerebral death by Doppler sonography (author's transl)]. Directional Doppler sonography of the supratrochlear and extracranial cerebral arteries was performed on 16 patients without any cerebral flow, as confirmed angiographically. The findings were inconsistent for the supratrochlear artery, but the internal carotid and vertebral arteries had characteristic changes in the Doppler curve with clearly diminished systolic flow velocity and flow reversal during diastole. These results were never seen in arteriosclerotic occlusion, hypoxaemic or toxic cerebral oedema. Doppler sonography thus helps to formulate indications for early cerebral angiography."}
{"id": "PMID:477568", "title": "[Possibilities of radiation-dose reduction in the treatment of Hodgkin's disease. I. Exclusion of the mediastinum from the radiation field in primary high-cervical or infradiaphragmatic involvement and stage I or II (author's transl)].", "content": "A retrospective analysis on 482 patients with localised Hodgkin's disease revealed that none of those with primary high-cervical or primary infradiaphragmatic involvement had any infiltration of the mediastinum, either at the time or later. As a result, prophylactic mediastinal radiation was not practised if high-cervical or infradiaphragmatic lymph-nodes had been affected first. None of these patients had any mediastinal recurrence after at least seven years.", "contents": "[Possibilities of radiation-dose reduction in the treatment of Hodgkin's disease. I. Exclusion of the mediastinum from the radiation field in primary high-cervical or infradiaphragmatic involvement and stage I or II (author's transl)]. A retrospective analysis on 482 patients with localised Hodgkin's disease revealed that none of those with primary high-cervical or primary infradiaphragmatic involvement had any infiltration of the mediastinum, either at the time or later. As a result, prophylactic mediastinal radiation was not practised if high-cervical or infradiaphragmatic lymph-nodes had been affected first. None of these patients had any mediastinal recurrence after at least seven years."}
{"id": "PMID:477569", "title": "[Bacteria of the campylobacter species as cause of enteritis (author's transl)].", "content": "In an eight-year-old boy who had acute gastroenteritis, examination of the faeces with specific isolation technique revealed Campylobacter fetus subspecies jejuni as the responsible pathogen. This is the first observation in the Federal Republic of Germany of an enteritis caused by Campylobacter.", "contents": "[Bacteria of the campylobacter species as cause of enteritis (author's transl)]. In an eight-year-old boy who had acute gastroenteritis, examination of the faeces with specific isolation technique revealed Campylobacter fetus subspecies jejuni as the responsible pathogen. This is the first observation in the Federal Republic of Germany of an enteritis caused by Campylobacter."}
{"id": "PMID:477573", "title": "Individualisation of oral anticoagulant therapy.", "content": "The hepatic synthesis of vitamin K dependent coagulation factors is modified by oral anticoagulant drugs, resulting in the release of functionally deficient coagulation factors into the circulation and consequently anticoagulation. Since their introduction into clinical medicine over 30 years ago, both clinical and scientific evidence has demonstrated the value of oral anticoagulants in the treatment and prophylaxis of venous thrombosis. In the treatment of arterial disease, however, both the indications for and usefulness of oral anticoagulants remain very much in doubt despite their widespread use in the 1950s and 1960s and in numerous clinical trials. The initiation and continuation of oral anticoagulant therapy is a co-operative venture involving the patient, the clinician and the laboratory. The clinician must have a thorough knowledge of the indications for and contraindications to the use of these drugs, and regular, accurate laboratory control is essential if haemorrhage, the major side effect, is to be avoided or reduced to a minimum. The patient must bear the responsibility for regular clinic attendance, abstinence from proprietary medications, and must immediately seek medical advice if any sign of haemorrhage occurs.", "contents": "Individualisation of oral anticoagulant therapy. The hepatic synthesis of vitamin K dependent coagulation factors is modified by oral anticoagulant drugs, resulting in the release of functionally deficient coagulation factors into the circulation and consequently anticoagulation. Since their introduction into clinical medicine over 30 years ago, both clinical and scientific evidence has demonstrated the value of oral anticoagulants in the treatment and prophylaxis of venous thrombosis. In the treatment of arterial disease, however, both the indications for and usefulness of oral anticoagulants remain very much in doubt despite their widespread use in the 1950s and 1960s and in numerous clinical trials. The initiation and continuation of oral anticoagulant therapy is a co-operative venture involving the patient, the clinician and the laboratory. The clinician must have a thorough knowledge of the indications for and contraindications to the use of these drugs, and regular, accurate laboratory control is essential if haemorrhage, the major side effect, is to be avoided or reduced to a minimum. The patient must bear the responsibility for regular clinic attendance, abstinence from proprietary medications, and must immediately seek medical advice if any sign of haemorrhage occurs."}
{"id": "PMID:477572", "title": "Nomifensine: A review of its pharmacological properties and therapeutic efficacy in depressive illness.", "content": "Nomifensine is a tetrahydoisoquinoline antidepressant which is chemically unrelated to the tricyclic or tetracyclic antidepressants, the monoamine oxidase inhibitors or the recently introduced agents. Nomifensine resembles the tricyclic antidepressants in many of its pharmacological effects in animal models of depressive illness, but differs from them in that it strongly inhibits the re-uptake of dopamine as well as noradrenaline and is a relatively weak inhibitor of serotonin uptake. It has an overall efficacy comparable with that of imipramine and amitriptyline in depressive illness, but at dosages which have achieved a similar overall clinical improvement, nomifensine causes little or no sedation, fewer and milder anticholinergic side effects, and also appears less likely than these drugs to cause serious cardiotoxicity on overdosage. Nomifensine may aggravate the psychopathology of patients with schizo-affective disorders and intensification of the psychosis may require neuroleptic therapy. Nomifensine also has antianxiety activity, but its role in treating anxiety associated with primary depression has still to be clarified. Nomifensine appears to be well tolerated by the elderly.", "contents": "Nomifensine: A review of its pharmacological properties and therapeutic efficacy in depressive illness. Nomifensine is a tetrahydoisoquinoline antidepressant which is chemically unrelated to the tricyclic or tetracyclic antidepressants, the monoamine oxidase inhibitors or the recently introduced agents. Nomifensine resembles the tricyclic antidepressants in many of its pharmacological effects in animal models of depressive illness, but differs from them in that it strongly inhibits the re-uptake of dopamine as well as noradrenaline and is a relatively weak inhibitor of serotonin uptake. It has an overall efficacy comparable with that of imipramine and amitriptyline in depressive illness, but at dosages which have achieved a similar overall clinical improvement, nomifensine causes little or no sedation, fewer and milder anticholinergic side effects, and also appears less likely than these drugs to cause serious cardiotoxicity on overdosage. Nomifensine may aggravate the psychopathology of patients with schizo-affective disorders and intensification of the psychosis may require neuroleptic therapy. Nomifensine also has antianxiety activity, but its role in treating anxiety associated with primary depression has still to be clarified. Nomifensine appears to be well tolerated by the elderly."}
{"id": "PMID:477588", "title": "[Disorders of cognitive activity in schizophrenics].", "content": "4 tests are exploring the cognitive activity of 3 groups of persons: normal, mental patients of various types, schizophrenics, homogeneous as far as the I.Q. is concerned (above 110) and education (secondary school, or university). Whereas normal and mental patients give identical results, except that they are worse for the latter, schizophrenics have better success than other patients in two tests of logic-mathematical reasoning and obviously worse in two tests of experimental logic. These results are interpreted in the frame of Piaget's theory as demonstrating the discordance of the very dynamics of schizophrenic thinking whose cognitive activity is at the same time too near to the object by adherence to the perceived structure (too concrete) and too far from it by adherence to formal reasoning schemes acquired under genetic development (too abstract). These results are coherent with clinical features showing that autistic thinking is not only discordant by its contents and its meaning, but also by the formal dynamic patterns of its modus operandi.", "contents": "[Disorders of cognitive activity in schizophrenics]. 4 tests are exploring the cognitive activity of 3 groups of persons: normal, mental patients of various types, schizophrenics, homogeneous as far as the I.Q. is concerned (above 110) and education (secondary school, or university). Whereas normal and mental patients give identical results, except that they are worse for the latter, schizophrenics have better success than other patients in two tests of logic-mathematical reasoning and obviously worse in two tests of experimental logic. These results are interpreted in the frame of Piaget's theory as demonstrating the discordance of the very dynamics of schizophrenic thinking whose cognitive activity is at the same time too near to the object by adherence to the perceived structure (too concrete) and too far from it by adherence to formal reasoning schemes acquired under genetic development (too abstract). These results are coherent with clinical features showing that autistic thinking is not only discordant by its contents and its meaning, but also by the formal dynamic patterns of its modus operandi."}
{"id": "PMID:477589", "title": "[Abnormal movements induced by psychotropic drugs].", "content": "The abnormal movements produced by the psychotropic drugs are related to various physiopathological mechanisms: -- dopaminergic receptors blockage provokes neuroleptic parkinsoniam tremor; -- neuroleptic-induced tardive dyskinesia is similar to 1. Dopa abnormal movements; tardive dyskinesia is due to denervation induced hypersensitivity of the dopamine receptor; the 1. Dopa dyskinesia is probably related to altered responsiveness of these receptors due to an increase in dopaminergic receptor sensitivity; -- antidepressant drugs, particularly lithium, provoke tremor, alleviated by beta blockage; it is probably caused by an abnormal sensitivity of the beta noradrenergic receptors.", "contents": "[Abnormal movements induced by psychotropic drugs]. The abnormal movements produced by the psychotropic drugs are related to various physiopathological mechanisms: -- dopaminergic receptors blockage provokes neuroleptic parkinsoniam tremor; -- neuroleptic-induced tardive dyskinesia is similar to 1. Dopa abnormal movements; tardive dyskinesia is due to denervation induced hypersensitivity of the dopamine receptor; the 1. Dopa dyskinesia is probably related to altered responsiveness of these receptors due to an increase in dopaminergic receptor sensitivity; -- antidepressant drugs, particularly lithium, provoke tremor, alleviated by beta blockage; it is probably caused by an abnormal sensitivity of the beta noradrenergic receptors."}
{"id": "PMID:477591", "title": "[The psychosis-epilepsy relationship in children].", "content": "From four cases studied both clinically and with the help of repeated and prolonged electroencephalograms, the problems raised by the association psychosis-epilepsy in children are reconsidered. The difficulties in evaluating: the actual weight of the \"epilepsy\" component; the role of the epileptic discharge for the subject's psychic economy; the role of drug therapies, are particularly underlined.", "contents": "[The psychosis-epilepsy relationship in children]. From four cases studied both clinically and with the help of repeated and prolonged electroencephalograms, the problems raised by the association psychosis-epilepsy in children are reconsidered. The difficulties in evaluating: the actual weight of the \"epilepsy\" component; the role of the epileptic discharge for the subject's psychic economy; the role of drug therapies, are particularly underlined."}
{"id": "PMID:477590", "title": "[Contribution of radiography and gammagraphy to the monitoring of the development of psychoses].", "content": "The authors emphasize the frequency of intracranial tumors in psychiatric patients. They make a distinction between the rare cases of psychiatric patients with intracranial tumors and the even most-frequent cases of tumors that express themselves through psychiatric disorders. According to the authors, the most important detection-tests are the brain scan and, more recently, the EMI scan or tomodensitometer.", "contents": "[Contribution of radiography and gammagraphy to the monitoring of the development of psychoses]. The authors emphasize the frequency of intracranial tumors in psychiatric patients. They make a distinction between the rare cases of psychiatric patients with intracranial tumors and the even most-frequent cases of tumors that express themselves through psychiatric disorders. According to the authors, the most important detection-tests are the brain scan and, more recently, the EMI scan or tomodensitometer."}
{"id": "PMID:477592", "title": "[Mental disorders and prolactin secretion].", "content": "Recent reports suggest that selective endocrine disturbances are often associated with mental disease, and that hormonal responses to treatments with antipsychotic drugs can be related to their clinical efficiency. The importance of central monoamine neuron systems for both hormonal control and mental disease probably accounts for these correlations. Measurement of prolactin, a hormone primarily regulated by hypothalamic dopaminergic neurons, provides a useful tool which can help to define the symptomatology of schizophrenic or depressed patients, to calibrate drug therapy and to obtain advance warning of therapeutical side effects. The hormone can also be used as an excellent index for screening dopamine agonist or antagonist properties of new drugs.", "contents": "[Mental disorders and prolactin secretion]. Recent reports suggest that selective endocrine disturbances are often associated with mental disease, and that hormonal responses to treatments with antipsychotic drugs can be related to their clinical efficiency. The importance of central monoamine neuron systems for both hormonal control and mental disease probably accounts for these correlations. Measurement of prolactin, a hormone primarily regulated by hypothalamic dopaminergic neurons, provides a useful tool which can help to define the symptomatology of schizophrenic or depressed patients, to calibrate drug therapy and to obtain advance warning of therapeutical side effects. The hormone can also be used as an excellent index for screening dopamine agonist or antagonist properties of new drugs."}
{"id": "PMID:477593", "title": "[Psychiatric and postsurographic approach in the study of post-concussion syndromes following head or whiplash injuries. Correlation and additional aspects].", "content": "Purpose of this paper is to point out the interest in case of post-concussional syndroma following an head or whiplash injury to practice both: a posturographic examination objectiving the functioning of the different sensori-motor systems who maintain the body equilibrium giving an approach of the possibility of an organic etiology, psychiatric one giving the psychogenic aspect. Different therapeutics are proposed in regard of this double approach. Four cases illustrate this purpose.", "contents": "[Psychiatric and postsurographic approach in the study of post-concussion syndromes following head or whiplash injuries. Correlation and additional aspects]. Purpose of this paper is to point out the interest in case of post-concussional syndroma following an head or whiplash injury to practice both: a posturographic examination objectiving the functioning of the different sensori-motor systems who maintain the body equilibrium giving an approach of the possibility of an organic etiology, psychiatric one giving the psychogenic aspect. Different therapeutics are proposed in regard of this double approach. Four cases illustrate this purpose."}
{"id": "PMID:477594", "title": "Depot neuroleptics in the treatment of acute psychoses.", "content": "The data reported in the present study are derived from clinical records of 43 acute inpatients treated with the depot neuroleptic F.D. during a one year period, at the Psychiatric Institute of the University of Pisa. The criteria of selection of acute patients of schizophrenic, manic and schizoaffective type are reported. With such treatment it was possible to eliminate the stressful forced repeated drug administrations without any particular side-effects and the drug combinations were greatley reduced. However the clinical observations of this study need further confirm.", "contents": "Depot neuroleptics in the treatment of acute psychoses. The data reported in the present study are derived from clinical records of 43 acute inpatients treated with the depot neuroleptic F.D. during a one year period, at the Psychiatric Institute of the University of Pisa. The criteria of selection of acute patients of schizophrenic, manic and schizoaffective type are reported. With such treatment it was possible to eliminate the stressful forced repeated drug administrations without any particular side-effects and the drug combinations were greatley reduced. However the clinical observations of this study need further confirm."}
{"id": "PMID:477595", "title": "[An epidemiologic approach to psychoses].", "content": "This study is an epidemiological approach of psychoses based on morbidity statistics from french psyschiatric hospitals for the years 1968, 1971 and 1975. It compares some of the most interesting census and first admission data for the following categories: manic-depressive psychoses, chronic schizophrenia and paranoid states on the one hand, neurotic conditions, personality disorders and non-psychotic depressions on the other. Major trends are analysed. Two of them seem particularly interesting: 1) the decreasing number of cases classified as manic-depressive psychoses; 2) the diminishing proportion of females in most instances. Some explanatory hypotheses are suggested.", "contents": "[An epidemiologic approach to psychoses]. This study is an epidemiological approach of psychoses based on morbidity statistics from french psyschiatric hospitals for the years 1968, 1971 and 1975. It compares some of the most interesting census and first admission data for the following categories: manic-depressive psychoses, chronic schizophrenia and paranoid states on the one hand, neurotic conditions, personality disorders and non-psychotic depressions on the other. Major trends are analysed. Two of them seem particularly interesting: 1) the decreasing number of cases classified as manic-depressive psychoses; 2) the diminishing proportion of females in most instances. Some explanatory hypotheses are suggested."}
{"id": "PMID:477596", "title": "Studies of the biological and immunological properties of parathyroid hormone, labeled selectively on the methionine residues by [3H]methyl exchange to high specific activity.", "content": "A technique is described for labeling bovine parathyroid hormone (bPTH) with tritium by [3Hmethyl exchange. The methionine residues were first methylated with [3H]methyl iodide at pH 4, and the reaction products were separated by cation exchange chromatography. The major peak consisted to hormone in which both methionines were converted to [3H]methyl methionine sulfonium iodide (3H-methylated bPTH). This product was then demethylated with 2-mercaptoethanol (6 M) at pH 8.6 to regenerate the hormone in an unmodified but tritiated form ([3H]bPTH), with a specific activity of 1.7 Ci/mmol. High pressure liquid chromatographic analysis showed that 96% of the radioactivity was incorporated into the methionine residues. There was no evidence of any alteration in the primary structure, as [3H]bPTH was found to run in the same position as unlabeled bPTH on cation exchange chromatography and disc gel electrophoresis and to have an identical absorption spectrum in the 240- to 330-nm range. Moreover, [3H]bPTH had full biological activity, as measured by an in vitro bioassay based on activation of rat renal cortical adenylate cyclase, although 3H-methylated bPTH was almost completely inactive. Similarly, while 3H-methylated bPTH had reduced potency in a RIA specific for antigenic sites in the 1--34 region of the sequence, [3H]bPTH was found to have full activity. The preparation of labeled bPTH was repeated using [14C]methyl iodide, with similar results, although [14C]bPTH was found to have somewhat reduced immunological and biological activities. While [3H]bPTH had a lower specific activity than can be obtained by various other techniques for incorporating tritium or 125I into peptides, biosynthetic labeling is at present the only alternative method for preparing biologically active, labeled bPTH without altering the primary structure. By comparison with this technique, the present method gave a product of a much higher specific activity which was labeled specifically in the biologically essential amino-terminal region. The same simple chemical procedures are clearly of wide potential application to the preparation of other labeled peptides.", "contents": "Studies of the biological and immunological properties of parathyroid hormone, labeled selectively on the methionine residues by [3H]methyl exchange to high specific activity. A technique is described for labeling bovine parathyroid hormone (bPTH) with tritium by [3Hmethyl exchange. The methionine residues were first methylated with [3H]methyl iodide at pH 4, and the reaction products were separated by cation exchange chromatography. The major peak consisted to hormone in which both methionines were converted to [3H]methyl methionine sulfonium iodide (3H-methylated bPTH). This product was then demethylated with 2-mercaptoethanol (6 M) at pH 8.6 to regenerate the hormone in an unmodified but tritiated form ([3H]bPTH), with a specific activity of 1.7 Ci/mmol. High pressure liquid chromatographic analysis showed that 96% of the radioactivity was incorporated into the methionine residues. There was no evidence of any alteration in the primary structure, as [3H]bPTH was found to run in the same position as unlabeled bPTH on cation exchange chromatography and disc gel electrophoresis and to have an identical absorption spectrum in the 240- to 330-nm range. Moreover, [3H]bPTH had full biological activity, as measured by an in vitro bioassay based on activation of rat renal cortical adenylate cyclase, although 3H-methylated bPTH was almost completely inactive. Similarly, while 3H-methylated bPTH had reduced potency in a RIA specific for antigenic sites in the 1--34 region of the sequence, [3H]bPTH was found to have full activity. The preparation of labeled bPTH was repeated using [14C]methyl iodide, with similar results, although [14C]bPTH was found to have somewhat reduced immunological and biological activities. While [3H]bPTH had a lower specific activity than can be obtained by various other techniques for incorporating tritium or 125I into peptides, biosynthetic labeling is at present the only alternative method for preparing biologically active, labeled bPTH without altering the primary structure. By comparison with this technique, the present method gave a product of a much higher specific activity which was labeled specifically in the biologically essential amino-terminal region. The same simple chemical procedures are clearly of wide potential application to the preparation of other labeled peptides."}
{"id": "PMID:477597", "title": "Neonatal estrogen treatment alters sexual differentiation of hepatic histidase.", "content": "Hepatic histidase was used as an enzyme marker for the study of neonatal programming in intact rats. Diethylstilbestrol (DES) or 17 beta-estradiol (E2) treatment for days, 2, 4, and 6 post partum resulted in decreased histidase activities in the adult female, but no effect was seen in prepubertal male and female rats or in adult males. In contrast, similar neonatal doses of testosterone propionate (TP) had no effect on histidase. Dose-response experiments demonstrate a 3-fold greater neonatal sensitivity to DES than to E2. The action of neonatal estrogen treatment is demonstrated to be permanent and irreversible. Neonatal treatment with E2, DES, or TP resulted in decreased uterine wet weights in adult females (E2 less than DES less than TP less than controls). Circulating sera estrogen levels were lower in adult E2- and DES-treated females than in TP-treated and control females. Our results suggest that these alterations may be due to direct toxic effects on the postnatal development of the female reproductive tract and endocrine system and/or to organizational effects on nerve endings in the hypothalamus that result in programming for altered sexual differentiation of hepatic metabolism.", "contents": "Neonatal estrogen treatment alters sexual differentiation of hepatic histidase. Hepatic histidase was used as an enzyme marker for the study of neonatal programming in intact rats. Diethylstilbestrol (DES) or 17 beta-estradiol (E2) treatment for days, 2, 4, and 6 post partum resulted in decreased histidase activities in the adult female, but no effect was seen in prepubertal male and female rats or in adult males. In contrast, similar neonatal doses of testosterone propionate (TP) had no effect on histidase. Dose-response experiments demonstrate a 3-fold greater neonatal sensitivity to DES than to E2. The action of neonatal estrogen treatment is demonstrated to be permanent and irreversible. Neonatal treatment with E2, DES, or TP resulted in decreased uterine wet weights in adult females (E2 less than DES less than TP less than controls). Circulating sera estrogen levels were lower in adult E2- and DES-treated females than in TP-treated and control females. Our results suggest that these alterations may be due to direct toxic effects on the postnatal development of the female reproductive tract and endocrine system and/or to organizational effects on nerve endings in the hypothalamus that result in programming for altered sexual differentiation of hepatic metabolism."}
{"id": "PMID:477601", "title": "Ether releases large amounts of prolactin from rat pituitaries previously \"depleted\" by short term suckling.", "content": "The effect of ether inhalation upon plasma PRL levels was assessed in lactating rats whose pituitary stores of the hormone first had been depleted by a short term (10-min) period of suckling in comparison with those whose PRL stores had not been depleted by prior suckling. Ether, unlike suckling or handling-decapitation stress, did not deplete pituitary PRL and caused only a small increase (36 to 52 ng/ml) in the plasma concentration of the hormone. However, when ether was given 10, 60, or 120 min after the plasma concentration had subsided from a prior 10-min period of suckling, the plasma concentration of PRL in each instance rose within 5--10 min to 200--250 ng/ml and was sustained at that level for the remainder of the 30-min exposure period. The amplitude and profile of the plasma PRL concentration after ether inhalation were similar to those obtained when a second suckling, rather than ether, was administered after the short term suckling. These data support the hypothesis that releasable PRL derives from depleted PRL and also indicate that, once it is formed, releasable PRL remains available for discharge into the circulation for a relatively long period of time.", "contents": "Ether releases large amounts of prolactin from rat pituitaries previously \"depleted\" by short term suckling. The effect of ether inhalation upon plasma PRL levels was assessed in lactating rats whose pituitary stores of the hormone first had been depleted by a short term (10-min) period of suckling in comparison with those whose PRL stores had not been depleted by prior suckling. Ether, unlike suckling or handling-decapitation stress, did not deplete pituitary PRL and caused only a small increase (36 to 52 ng/ml) in the plasma concentration of the hormone. However, when ether was given 10, 60, or 120 min after the plasma concentration had subsided from a prior 10-min period of suckling, the plasma concentration of PRL in each instance rose within 5--10 min to 200--250 ng/ml and was sustained at that level for the remainder of the 30-min exposure period. The amplitude and profile of the plasma PRL concentration after ether inhalation were similar to those obtained when a second suckling, rather than ether, was administered after the short term suckling. These data support the hypothesis that releasable PRL derives from depleted PRL and also indicate that, once it is formed, releasable PRL remains available for discharge into the circulation for a relatively long period of time."}
{"id": "PMID:477604", "title": "Rapid inibition of basal and glucose-stimulated insulin release by xylazine.", "content": "Xylazine is a tranquilizer that is widely used in both biomedical research and veterinary medicine. We now report that in dogs, clinically effective doses of xylazine both markedly decrease basal insulin levels and completely abolish the rise in insulin produced by iv glucose. These changes in insulin levels lead to elevations of fasting plasma glucose and glucose intolerance. In contrast, glucagon levels are unchanged. These studies suggest that xylazine may be a useful agent in the study of glucose metabolism and beta-cell function.", "contents": "Rapid inibition of basal and glucose-stimulated insulin release by xylazine. Xylazine is a tranquilizer that is widely used in both biomedical research and veterinary medicine. We now report that in dogs, clinically effective doses of xylazine both markedly decrease basal insulin levels and completely abolish the rise in insulin produced by iv glucose. These changes in insulin levels lead to elevations of fasting plasma glucose and glucose intolerance. In contrast, glucagon levels are unchanged. These studies suggest that xylazine may be a useful agent in the study of glucose metabolism and beta-cell function."}
{"id": "PMID:477605", "title": "The effects of metergoline and other serotonin receptor antagonists on serum corticosterone in rats.", "content": "Metergoline antagonized the elevation of serum corticosterone by quipazine in rats. The ED50 of metergoline was less than 0.1 mg/kg, ip, and the effects of a 3 mg/kg dose persisted for more than 24 h. Metergoline did not antagonize the elevation of serum corticosterone by theophylline or ketamine (i.e. did not prevent corticosterone release nonspecifically) and did not affect the concentration of quipazine in the brain. Since quipazine is a serotonin receptor agonist, the antagonistic effects of metergoline may have been due to competition with quipazine at serotonin receptor sites in the brain. Some other agents capable of blocking serotonin receptors also antagonzied the elevation of serum corticosterone by quipazine. These included LY53857, which gave complete blockade at 3 mg/kg, and cyproheptadine and spiperone, which gave significant but incomplete antagonism at 1 mg/kg. Methysergide at 3 mg/kg did not alter the effect of quipazine. Metergoline did not antagonize the elevation of serum corticosterone by other agents throught to act via serotoninergic mechanisms, namely fluoxetine, fenfluramine, L-5-hydroxytryptophan, N,N-demthyl-5-methoxytryptamine, and 1-(m-trifluoromethylphenyl)piperazine. Thus, the interactions between metergoline and quipazine may have occurred at receptors that are not serotonin receptors or that represent a subset of serotonin receptors not mediating the actions of serotoninergic agents other than quipazine.", "contents": "The effects of metergoline and other serotonin receptor antagonists on serum corticosterone in rats. Metergoline antagonized the elevation of serum corticosterone by quipazine in rats. The ED50 of metergoline was less than 0.1 mg/kg, ip, and the effects of a 3 mg/kg dose persisted for more than 24 h. Metergoline did not antagonize the elevation of serum corticosterone by theophylline or ketamine (i.e. did not prevent corticosterone release nonspecifically) and did not affect the concentration of quipazine in the brain. Since quipazine is a serotonin receptor agonist, the antagonistic effects of metergoline may have been due to competition with quipazine at serotonin receptor sites in the brain. Some other agents capable of blocking serotonin receptors also antagonzied the elevation of serum corticosterone by quipazine. These included LY53857, which gave complete blockade at 3 mg/kg, and cyproheptadine and spiperone, which gave significant but incomplete antagonism at 1 mg/kg. Methysergide at 3 mg/kg did not alter the effect of quipazine. Metergoline did not antagonize the elevation of serum corticosterone by other agents throught to act via serotoninergic mechanisms, namely fluoxetine, fenfluramine, L-5-hydroxytryptophan, N,N-demthyl-5-methoxytryptamine, and 1-(m-trifluoromethylphenyl)piperazine. Thus, the interactions between metergoline and quipazine may have occurred at receptors that are not serotonin receptors or that represent a subset of serotonin receptors not mediating the actions of serotoninergic agents other than quipazine."}
{"id": "PMID:477606", "title": "Vitamin D-dependent calcium-binding protein of rat intestine: changes during postnatal development and sensitivity to 1,25-dihydroxycholecalciferol.", "content": "To study the role of the vitamin D-endocrine system during the perinatal period, we monitored vitamin D-dependent calcium-binding protein (CaBP) in rat intestine by radial immunodiffusion and polyacrylamide disc gel electrophoresis. Small amounts of CaBp were present 2 days before birth; these levels increased 74-fold by day 38 after birth. Approximately 80% of the increase in CaBP concentration occurred in a 5-day period at the time of weaning (days 17--22 after birth). Before this period, the concentration of CaBP was comparable to that found in rachitic (adult) rats. Administration of 1,25-dihydroxycholecalciferol to suckling rats on days 15 and/or 16 was followed by a premature increase in the amount of intestinal CaBP. These data demonstrate that although vitamin D-dependent CaBP is low in preweaned rats, the rat intestine is responsive to exogeneous 1,25-dihydroxyvitamin D3 at least as early as day 15 after birth. The close temporal correspondence between the increases in CaBP and previously reported changes in calcium transport and vitamin D metabolism suggest that the vitamin D-endocrine system plays a role in postnatal intestinal maturation and adaptation during the weaning period.", "contents": "Vitamin D-dependent calcium-binding protein of rat intestine: changes during postnatal development and sensitivity to 1,25-dihydroxycholecalciferol. To study the role of the vitamin D-endocrine system during the perinatal period, we monitored vitamin D-dependent calcium-binding protein (CaBP) in rat intestine by radial immunodiffusion and polyacrylamide disc gel electrophoresis. Small amounts of CaBp were present 2 days before birth; these levels increased 74-fold by day 38 after birth. Approximately 80% of the increase in CaBP concentration occurred in a 5-day period at the time of weaning (days 17--22 after birth). Before this period, the concentration of CaBP was comparable to that found in rachitic (adult) rats. Administration of 1,25-dihydroxycholecalciferol to suckling rats on days 15 and/or 16 was followed by a premature increase in the amount of intestinal CaBP. These data demonstrate that although vitamin D-dependent CaBP is low in preweaned rats, the rat intestine is responsive to exogeneous 1,25-dihydroxyvitamin D3 at least as early as day 15 after birth. The close temporal correspondence between the increases in CaBP and previously reported changes in calcium transport and vitamin D metabolism suggest that the vitamin D-endocrine system plays a role in postnatal intestinal maturation and adaptation during the weaning period."}
{"id": "PMID:477607", "title": "Mechanism of the dipsogenic action of tetradecapeptide renin substrate in dogs.", "content": "Dogs with chronically implanted third ventricular cannulae showed significant drinking responses to central injections of angiotensin II and tetradecapeptide renin substrate (TDP). The threshold dose for angiotensin II was 1 pmol and for TDP was 70 pmol. Although central injections of TDP led to drinking and appearance of angiotensin II in cerebrospinal fluid, renin substrate prepared from dog cerebrospinal fluid had no effect. The dipsogenic action of TDP was blocked by prior administration of converting enzyme inhibitor SQ20881 (P less than 0.01) but was not affected by either pepstatin or N-acetyl-pepstatin. Thus, converting enzyme acts directly on TDP to produce angiotensin I and then angiotensin II. The results of the present study do not provide evidence for the presence of an enzyme in the brain with renin-like activity.", "contents": "Mechanism of the dipsogenic action of tetradecapeptide renin substrate in dogs. Dogs with chronically implanted third ventricular cannulae showed significant drinking responses to central injections of angiotensin II and tetradecapeptide renin substrate (TDP). The threshold dose for angiotensin II was 1 pmol and for TDP was 70 pmol. Although central injections of TDP led to drinking and appearance of angiotensin II in cerebrospinal fluid, renin substrate prepared from dog cerebrospinal fluid had no effect. The dipsogenic action of TDP was blocked by prior administration of converting enzyme inhibitor SQ20881 (P less than 0.01) but was not affected by either pepstatin or N-acetyl-pepstatin. Thus, converting enzyme acts directly on TDP to produce angiotensin I and then angiotensin II. The results of the present study do not provide evidence for the presence of an enzyme in the brain with renin-like activity."}
{"id": "PMID:477608", "title": "The development of gonadotropin and steroid hormone patterns in male and female hamsters from birth to puberty.", "content": "Male (1--60 days old) and female (1--30 days old) hamsters were decapitated and serum levels of LH, FSH, PRL, progesterone, androgens (males), and estradiol (females) were measured by RIA. Males and females had similar levels of LH until 15 days of age and of FSH until 12 days of age, at which times gonadotropin levels increased significantly in females. Peak levels for females occurred on days 19--21 for LH and on days -2--24 for FSH, later than the times reported for female rats. Adjusting female gonadotropin peaks for gestation length places these peaks for hamsters and rats at the same time in postmating age. In female hamsters, large variations occur in LH between 16--25 days of age, as reported for female rats. Males reached peak serum levels of LH and FSH on day 40, just before the first motile epididymal sperm. Serum PRL levels were identical in male and female hamsters until at least day 30. PRL levels sharply increased in both sexes after day 18 and remained elevated until at least day 30. In males, serum androgens were low until 30 days of age, in contrast to high levels reported for infantile rats. Androgens rose sharply in male hamsters after day 30 to peak levels on day 50. Progesterone in males also remained low until after day 30. Serum estradiol in females did not attain the extremely high elevations seen in rats. Some fluctuations occurred between 10--30 days of age, which presumably represent maturational changes in the ovary. Serum progesterone in females followed a pattern of development similar to estradiol.", "contents": "The development of gonadotropin and steroid hormone patterns in male and female hamsters from birth to puberty. Male (1--60 days old) and female (1--30 days old) hamsters were decapitated and serum levels of LH, FSH, PRL, progesterone, androgens (males), and estradiol (females) were measured by RIA. Males and females had similar levels of LH until 15 days of age and of FSH until 12 days of age, at which times gonadotropin levels increased significantly in females. Peak levels for females occurred on days 19--21 for LH and on days -2--24 for FSH, later than the times reported for female rats. Adjusting female gonadotropin peaks for gestation length places these peaks for hamsters and rats at the same time in postmating age. In female hamsters, large variations occur in LH between 16--25 days of age, as reported for female rats. Males reached peak serum levels of LH and FSH on day 40, just before the first motile epididymal sperm. Serum PRL levels were identical in male and female hamsters until at least day 30. PRL levels sharply increased in both sexes after day 18 and remained elevated until at least day 30. In males, serum androgens were low until 30 days of age, in contrast to high levels reported for infantile rats. Androgens rose sharply in male hamsters after day 30 to peak levels on day 50. Progesterone in males also remained low until after day 30. Serum estradiol in females did not attain the extremely high elevations seen in rats. Some fluctuations occurred between 10--30 days of age, which presumably represent maturational changes in the ovary. Serum progesterone in females followed a pattern of development similar to estradiol."}
{"id": "PMID:477609", "title": "Estrogen receptor in the thymus of the castrated mice.", "content": "Sucrose density gradient ultracentrifugation and dextran-coated charcoal adsorption permitted us to characterize the estrogen-binding proteins in cytosols obtained from the thymus, spleen and mesenteric lymph node of the castrated male and female mice of C57BL strain. The thymic cytosol from both sexes incubated with 3H-estradiol-17 beta in the presence of excess unlabeled steroids showed a specific estrogenbinding 4 S protein with its binding capacity of 10(-14) moles/mg protein for males and 4 x 10(-15) moles/mg protein for females, respectively. The dissociation constant was of 4 x 10(-10) M for males and 3 x 10(-10) M for females, respectively. No specific binding was, however, found in the cytosols of the spleen and mesenteric lymph node. Steroid analysis by thin-layer chromatography of the thymic cytosols after incubation of them with 3H-estradiol-17 beta showed that a fair amount (around 60%) of radioactivity was from the undegradated radioactive steroid still bound to 4 S binder in both sexes. Enzyme study and heat experiment revealed that the estrogen specific 4 S binding component in the thymic cytosols bears at least protein in nature and is of heat-labile nature. These results strongly suggest that the thymus of the castrated mice contain a specific estrogen receptor, the nature of which is in part protein and heat-labile.", "contents": "Estrogen receptor in the thymus of the castrated mice. Sucrose density gradient ultracentrifugation and dextran-coated charcoal adsorption permitted us to characterize the estrogen-binding proteins in cytosols obtained from the thymus, spleen and mesenteric lymph node of the castrated male and female mice of C57BL strain. The thymic cytosol from both sexes incubated with 3H-estradiol-17 beta in the presence of excess unlabeled steroids showed a specific estrogenbinding 4 S protein with its binding capacity of 10(-14) moles/mg protein for males and 4 x 10(-15) moles/mg protein for females, respectively. The dissociation constant was of 4 x 10(-10) M for males and 3 x 10(-10) M for females, respectively. No specific binding was, however, found in the cytosols of the spleen and mesenteric lymph node. Steroid analysis by thin-layer chromatography of the thymic cytosols after incubation of them with 3H-estradiol-17 beta showed that a fair amount (around 60%) of radioactivity was from the undegradated radioactive steroid still bound to 4 S binder in both sexes. Enzyme study and heat experiment revealed that the estrogen specific 4 S binding component in the thymic cytosols bears at least protein in nature and is of heat-labile nature. These results strongly suggest that the thymus of the castrated mice contain a specific estrogen receptor, the nature of which is in part protein and heat-labile."}
{"id": "PMID:477612", "title": "Comparison of human calcitonin secretion after a 1-minute calcium infusion in young normal and in elderly subjects.", "content": "Human calcitonin (hCT) response to a 1-minute calcium infusion was studied in 6 male and 4 female young normal subjects and in 6 male and 6 female elderly subjects. In the young subjects plasma hCT levels increased significantly (p less than 0.01) from mean basal value of 82.3 +/- 48.7 pg/ml to maximum level of 407.5 +/-198.0 pg/ml in the male, while it increased from 96.3 +/- 89.2 to 216.3 +/- 89.1 pg/ml p less than 0.05) in the female. In the elderly subjects, it increased from 80.3 +/- 56.9 pg/ml to maximum level of 229.2 +/- 130.6 pg/ml (p less than 0.05) in the male, while it increased from 109.2 +/- 43.7 to 163.3 +/- 55.7 pg/ml (p less than 0.005) in the female. There was no significant difference in the basal hCT level of these 4 groups. In both the young and the elderly, however, mean increment of hCT level was significantly (p less than 0.05) higher in the male than in the female of the same-aged group. In both the female and the male subjects, it was significantly (p less than 0.05) higher in the young than in the elderly of the same sex. A 1-minute infusion of calcium can be used as a reliable provocative test for hCT secretion in human subjects.", "contents": "Comparison of human calcitonin secretion after a 1-minute calcium infusion in young normal and in elderly subjects. Human calcitonin (hCT) response to a 1-minute calcium infusion was studied in 6 male and 4 female young normal subjects and in 6 male and 6 female elderly subjects. In the young subjects plasma hCT levels increased significantly (p less than 0.01) from mean basal value of 82.3 +/- 48.7 pg/ml to maximum level of 407.5 +/-198.0 pg/ml in the male, while it increased from 96.3 +/- 89.2 to 216.3 +/- 89.1 pg/ml p less than 0.05) in the female. In the elderly subjects, it increased from 80.3 +/- 56.9 pg/ml to maximum level of 229.2 +/- 130.6 pg/ml (p less than 0.05) in the male, while it increased from 109.2 +/- 43.7 to 163.3 +/- 55.7 pg/ml (p less than 0.005) in the female. There was no significant difference in the basal hCT level of these 4 groups. In both the young and the elderly, however, mean increment of hCT level was significantly (p less than 0.05) higher in the male than in the female of the same-aged group. In both the female and the male subjects, it was significantly (p less than 0.05) higher in the young than in the elderly of the same sex. A 1-minute infusion of calcium can be used as a reliable provocative test for hCT secretion in human subjects."}
{"id": "PMID:477613", "title": "Thyroglobulin and microsomal antibodies in patients with insulin dependent diabetes mellitus and their relatives.", "content": "The sera for 88 parents and 9 siblings of 73 patients with insulin dependent diabetes mellitus in childhood and 437 controls matched in age and sex, were tested by the thyroglobulin and microsome-coated tanned red cell hemagglutination test (Fuji-Zoki Co. Tokyo). None of 73 children with diabetes mellitus had antithyroglobulin antibodies, whereas twelve (16.4%) had antimicrosomal antibodies compared with the incidence of 0.4% and 1.1%, respectively, in 437 controls. In the parents and siblings of these probands, thyroid antibodies were also found in increased incidence. The incidence of antimicrosomal antibodies in the 68 mothers was significantly higher than in controls matched for age and sex, but the incidence of the positive thyroid antibodies in the 20 fathers and 9 siblings was not significantly different from that in control populations. The incidence of thyroid antibodies tended to be higher, though not significant, in parents and siblings of diabetic children with positive thyroid antibodies than in those of diabetics with negative ones. These findings suggest that immunogenetic factors may be responsible for the pathogenesis of some cases of diabetes mellitus in childhood.", "contents": "Thyroglobulin and microsomal antibodies in patients with insulin dependent diabetes mellitus and their relatives. The sera for 88 parents and 9 siblings of 73 patients with insulin dependent diabetes mellitus in childhood and 437 controls matched in age and sex, were tested by the thyroglobulin and microsome-coated tanned red cell hemagglutination test (Fuji-Zoki Co. Tokyo). None of 73 children with diabetes mellitus had antithyroglobulin antibodies, whereas twelve (16.4%) had antimicrosomal antibodies compared with the incidence of 0.4% and 1.1%, respectively, in 437 controls. In the parents and siblings of these probands, thyroid antibodies were also found in increased incidence. The incidence of antimicrosomal antibodies in the 68 mothers was significantly higher than in controls matched for age and sex, but the incidence of the positive thyroid antibodies in the 20 fathers and 9 siblings was not significantly different from that in control populations. The incidence of thyroid antibodies tended to be higher, though not significant, in parents and siblings of diabetic children with positive thyroid antibodies than in those of diabetics with negative ones. These findings suggest that immunogenetic factors may be responsible for the pathogenesis of some cases of diabetes mellitus in childhood."}
{"id": "PMID:477614", "title": "Effects of vasoactive intestinal peptide on glycogenolysis in cultured liver cells.", "content": "When isolated rat liver cells were incubated in the presence of vasoactive intestinal peptide at the concentrations ranging from 0.2 microgram to 2 micrograms per ml, glycogenolysis was maximally stimulated within 15 min. However, somatostatin inhibited the liver glycogenolysis. The combined addition to the incubation medium showed that insulin and somatostatin inhibited the stimulated glycogenolysis induced by vasoactive intestinal peptide, while vasoactive intestinal peptide plus secretin showed no additive effect on glycogenolysis, as compared with single the addition of vasoactive intestinal peptide. On the other hand, the additon of glucagon to vasoactive intestinal peptide showed additive effects on glycogenolysis. These results suggest that the receptor site for vasoactive intestinal peptide may be distinguishable from that for glucagon. Extracellular calcium ions were demonstrated to play an important role in the modulation of vasoactive intestinal peptide-induced glycogenolysis. The evidence presented in this paper indicates that glucose metabolism may be partly regulated by the direct action of vasoactive intestinal peptide on hepatocytes, which is referred to as an enterohepatic axis and that the axis is inhibited by insulin and somatostatin.", "contents": "Effects of vasoactive intestinal peptide on glycogenolysis in cultured liver cells. When isolated rat liver cells were incubated in the presence of vasoactive intestinal peptide at the concentrations ranging from 0.2 microgram to 2 micrograms per ml, glycogenolysis was maximally stimulated within 15 min. However, somatostatin inhibited the liver glycogenolysis. The combined addition to the incubation medium showed that insulin and somatostatin inhibited the stimulated glycogenolysis induced by vasoactive intestinal peptide, while vasoactive intestinal peptide plus secretin showed no additive effect on glycogenolysis, as compared with single the addition of vasoactive intestinal peptide. On the other hand, the additon of glucagon to vasoactive intestinal peptide showed additive effects on glycogenolysis. These results suggest that the receptor site for vasoactive intestinal peptide may be distinguishable from that for glucagon. Extracellular calcium ions were demonstrated to play an important role in the modulation of vasoactive intestinal peptide-induced glycogenolysis. The evidence presented in this paper indicates that glucose metabolism may be partly regulated by the direct action of vasoactive intestinal peptide on hepatocytes, which is referred to as an enterohepatic axis and that the axis is inhibited by insulin and somatostatin."}
{"id": "PMID:477616", "title": "The stimulatory roles of catecholamines and acetylcholine in the regulation of gonadotropin release in ovariectomized estrogen-primed rats.", "content": "Injections of 2 mg of progesterone into ovariectomized estrogen-primed rats significantly increased serum LH and FSH concentrations 3, 5 and 8 hr later. Receptor blockers of noradrenaline (NA), dopamine (DA) or acetylcholine (ACH), phenoxybenzamine (20 mg/kg body weight), pimozide (1mg/kg body weight) or atropine (700 mg/kg body weight), respectively, prevented the progesterone-induced gonadotropin release. On the other hand, none of them blocked the gonadotropin release following unilateral electrochemical stimulation (100 microA for 60 sec) of the medial preoptic area which occurred 0.5 and 1.5 hr later, although pimozide or atropine reduced serum LH concentrations at 4.0 hr after stimulation. Furthermore, the sites of action of NA, DA and ACH with respect to LH release were examined by intracerebral implantation in ovariectomized estrogen-primed rats DA or ACH, when implanted unilaterally into the medial preoptic urea, induced a significant increase in serum LH 5 hr later, whereas NA decreased LH levels. Implantations of NA or ACH into the bed nucleus of the stria terminalis or the medial amygdala increased serum LH although the effect of NA into the latter was not statistically significant. Only implantations of NA among the three substances into the lateral septum induced LH release. These results suggest that all of NA, DA and ACH play stimulatory roles in the regulation of gonadotropin secretion, and that there are regional differences of their effectivenesses in releasing LH within the limbic-preoptic area.", "contents": "The stimulatory roles of catecholamines and acetylcholine in the regulation of gonadotropin release in ovariectomized estrogen-primed rats. Injections of 2 mg of progesterone into ovariectomized estrogen-primed rats significantly increased serum LH and FSH concentrations 3, 5 and 8 hr later. Receptor blockers of noradrenaline (NA), dopamine (DA) or acetylcholine (ACH), phenoxybenzamine (20 mg/kg body weight), pimozide (1mg/kg body weight) or atropine (700 mg/kg body weight), respectively, prevented the progesterone-induced gonadotropin release. On the other hand, none of them blocked the gonadotropin release following unilateral electrochemical stimulation (100 microA for 60 sec) of the medial preoptic area which occurred 0.5 and 1.5 hr later, although pimozide or atropine reduced serum LH concentrations at 4.0 hr after stimulation. Furthermore, the sites of action of NA, DA and ACH with respect to LH release were examined by intracerebral implantation in ovariectomized estrogen-primed rats DA or ACH, when implanted unilaterally into the medial preoptic urea, induced a significant increase in serum LH 5 hr later, whereas NA decreased LH levels. Implantations of NA or ACH into the bed nucleus of the stria terminalis or the medial amygdala increased serum LH although the effect of NA into the latter was not statistically significant. Only implantations of NA among the three substances into the lateral septum induced LH release. These results suggest that all of NA, DA and ACH play stimulatory roles in the regulation of gonadotropin secretion, and that there are regional differences of their effectivenesses in releasing LH within the limbic-preoptic area."}
{"id": "PMID:477618", "title": "Effects of hypothalamic deafferentation on hormonal facilitation of lordosis in ovariectomized rats.", "content": "Lordosis response in estradiol benzoate (EB)-progesterone (P) primed castrated female rats was studied after 4 types of deafferentation of the medial hypothalamus. Tests were started 4-5 weeks after the brain surgery and ovariectomy. Anterior deafferentation (half-dome cut) at the level behind the suprachiasmatic nucleus (AD-I) and island isolation of the medial basal hypothalamus (MBH island) were highly effective to suppress lordosis response in EB-P primed females. However, the cuts placed dorsal (AD-II) or posterior (AD-III) to the AD-I were without apparent suppressive effect on the EB-P induced display of lordosis. These results suggest that the anterolateral fiber connection with the MBH may participate in lordosis facilitatory mechanisms in EB-P primed females. When reserpine (R) was injected instead of P to EB-primed rats, R effectively facilitated lordosis response in rats with or without the deafferentation (AD-I or MBH island). This suggests that the MBH is not necessary to facilitate lordosis in EB-R primed rats. The site of action of R in the central nervous system for facilitating lordosis may not be the same as that of P.", "contents": "Effects of hypothalamic deafferentation on hormonal facilitation of lordosis in ovariectomized rats. Lordosis response in estradiol benzoate (EB)-progesterone (P) primed castrated female rats was studied after 4 types of deafferentation of the medial hypothalamus. Tests were started 4-5 weeks after the brain surgery and ovariectomy. Anterior deafferentation (half-dome cut) at the level behind the suprachiasmatic nucleus (AD-I) and island isolation of the medial basal hypothalamus (MBH island) were highly effective to suppress lordosis response in EB-P primed females. However, the cuts placed dorsal (AD-II) or posterior (AD-III) to the AD-I were without apparent suppressive effect on the EB-P induced display of lordosis. These results suggest that the anterolateral fiber connection with the MBH may participate in lordosis facilitatory mechanisms in EB-P primed females. When reserpine (R) was injected instead of P to EB-primed rats, R effectively facilitated lordosis response in rats with or without the deafferentation (AD-I or MBH island). This suggests that the MBH is not necessary to facilitate lordosis in EB-R primed rats. The site of action of R in the central nervous system for facilitating lordosis may not be the same as that of P."}
{"id": "PMID:477619", "title": "The release and metabolism of pancreatic hormones after major hepatectomy in the dog.", "content": "Major hepatectomy in the dog induced a 50% decrease in peripheral serum glucose, a 11-fold increase in portal plasma glucagon and a 36-fold increase in the portal glucagon/insulin ratio 3 hr after operation. Peripheral serum glucose levels were inversely correlated to the logarithmic value of portal plasma glucagon (r = -0.50, p less than 0.01) and that of the portal glucagon/insulin ratio (r = -0.85, p less than 0.01) for 1-6 hr after operation. The ratio of peripheral to portal plasma glucagon was also inversely correlated to the logarithmic value of portal plasma glucagon (r = -0.59, p less than 0.01). In case of glucose infusion, plasma glucagon levels were not elevated after major hepatectomy. The data suggest that glucose deficiency after major hepatectomy in the dog may cause hyperglucagonemia with an enhanced glucagon requirement.", "contents": "The release and metabolism of pancreatic hormones after major hepatectomy in the dog. Major hepatectomy in the dog induced a 50% decrease in peripheral serum glucose, a 11-fold increase in portal plasma glucagon and a 36-fold increase in the portal glucagon/insulin ratio 3 hr after operation. Peripheral serum glucose levels were inversely correlated to the logarithmic value of portal plasma glucagon (r = -0.50, p less than 0.01) and that of the portal glucagon/insulin ratio (r = -0.85, p less than 0.01) for 1-6 hr after operation. The ratio of peripheral to portal plasma glucagon was also inversely correlated to the logarithmic value of portal plasma glucagon (r = -0.59, p less than 0.01). In case of glucose infusion, plasma glucagon levels were not elevated after major hepatectomy. The data suggest that glucose deficiency after major hepatectomy in the dog may cause hyperglucagonemia with an enhanced glucagon requirement."}
{"id": "PMID:477620", "title": "Insulin and glucagon relationships during aging in rats.", "content": "Oral glucose tolerance tests were performed under pentobarbital anesthesia in 43 male Wistar rats 2 to 18 months of age in order to determine if insulin and glucagon secretion are altered with aging. Although any linear correlation was not demonstrated between aging and blood glucose, plasma insulin or glucagon levels, post-glucose levels of blood glucose were significantly suppressed and those of plasma glucagon were significantly elevated at 4 to 6 months of age. No significant difference was found between young (2 months of age) and aged rats (12 to 14 and 17 to 18 months of age) in either blood glucose or plasma insulin levels during oral glucose load. On the other hand, post-glucose plasma glucagon levels of the aged rats were significantly higher than those of the young ones. Furthermore, comparisons of various kinds of indices among the different age groups, such as insulinogenic index, insulin/glucagon and so forth during oral glucose tolerance tests also indicate the significant alteration of glucagon secretion during aging process. It is concluded from the present data that glucose tolerance does not apparently deteriorate during aging process in rats but that glucagon responses to oral glucose administration are elevated with aging.", "contents": "Insulin and glucagon relationships during aging in rats. Oral glucose tolerance tests were performed under pentobarbital anesthesia in 43 male Wistar rats 2 to 18 months of age in order to determine if insulin and glucagon secretion are altered with aging. Although any linear correlation was not demonstrated between aging and blood glucose, plasma insulin or glucagon levels, post-glucose levels of blood glucose were significantly suppressed and those of plasma glucagon were significantly elevated at 4 to 6 months of age. No significant difference was found between young (2 months of age) and aged rats (12 to 14 and 17 to 18 months of age) in either blood glucose or plasma insulin levels during oral glucose load. On the other hand, post-glucose plasma glucagon levels of the aged rats were significantly higher than those of the young ones. Furthermore, comparisons of various kinds of indices among the different age groups, such as insulinogenic index, insulin/glucagon and so forth during oral glucose tolerance tests also indicate the significant alteration of glucagon secretion during aging process. It is concluded from the present data that glucose tolerance does not apparently deteriorate during aging process in rats but that glucagon responses to oral glucose administration are elevated with aging."}
{"id": "PMID:477621", "title": "Effects of oral administration of positively charged insulin liposomes on alloxan diabetic rats: preliminary study.", "content": "Insulin encapsulated in liposomes of various lipid compositions were prepared. The amount of insulin trapped in these liposomes increased in the order, negatively charged liposomes less than neutral liposomes less than positively charged liposomes. In positively charged liposomes, the amount of insulin trapped increased with increase in the amount of amphiphile stearylamine. Under the conditions tested, the highest insulin content (about 50%) was obtained with liposomes composed of phosphatidyl choline/cholesterol/stearylamine in a molar ratio of 7/2/2.25. These liposomes were stable on incubation for 3 hr at 37 degrees C in solutions of pepsin, trypsin, and pancreatin, and after these incubations, a considerable amount of insulin was still associated with the liposomes. However, the liposomes released almost all the insulin into the medium on treatment with bile. When the liposomes were administered orally to rats in the 3rd phase of acute alloxan diabetes, reduction of the blood glucose level was observed in 7 of 11 animals, the reduction persisted for several hours and was ranging from 30 to 75%. In alloxan diabetic rats showing hyperglycemia for 3 to 6 months, the liposomes also increased the glucose tolerance in half the animals tested.", "contents": "Effects of oral administration of positively charged insulin liposomes on alloxan diabetic rats: preliminary study. Insulin encapsulated in liposomes of various lipid compositions were prepared. The amount of insulin trapped in these liposomes increased in the order, negatively charged liposomes less than neutral liposomes less than positively charged liposomes. In positively charged liposomes, the amount of insulin trapped increased with increase in the amount of amphiphile stearylamine. Under the conditions tested, the highest insulin content (about 50%) was obtained with liposomes composed of phosphatidyl choline/cholesterol/stearylamine in a molar ratio of 7/2/2.25. These liposomes were stable on incubation for 3 hr at 37 degrees C in solutions of pepsin, trypsin, and pancreatin, and after these incubations, a considerable amount of insulin was still associated with the liposomes. However, the liposomes released almost all the insulin into the medium on treatment with bile. When the liposomes were administered orally to rats in the 3rd phase of acute alloxan diabetes, reduction of the blood glucose level was observed in 7 of 11 animals, the reduction persisted for several hours and was ranging from 30 to 75%. In alloxan diabetic rats showing hyperglycemia for 3 to 6 months, the liposomes also increased the glucose tolerance in half the animals tested."}
{"id": "PMID:477622", "title": "Sex hormones metabolism in the brain: influence of central acting drugs on 5 alpha-reduction in rat diencephalon.", "content": "Rats after adrenalectomy-testectomy showed a gradual increase in diencephalon 3-oxo-5 alpha-steroid: (acceptor) delta4-oxidoreductase (5 alpha-reductase) activity for 3 days. The activity then returned near to the normal range on the 4th postoperative day. When rats were given testosterone propionate (TP) 3 days after adrenalectomy-testectomy, diencephalon 5 alpha-reductase activity returned to the preoperative range 2 hr after TP administration. Diencephalon 5 alpha-reductase activity showed a highly significant increase (p less than 0.01) after a single administration of carbamazepine, reserpine, diazepam, phenytoin, phenobarbital or disulfiram. A significant increase (p less than 0.05) was also found after a single administration of methylphenidate, caffeine or methamphetamine. Plasma testosterone decreased concurrently after administration of all these agents, except diazepam. Diencephalon enzyme activity decreased significantly after repeated disulfiram administrations (p less than 0.01) but increased significantly after methamphetamine administrations (p less than 0.05). Plasma testosterone showed a tendency to decrease after repeated methamphetamine administrations but tended to increase after repeated disulfiram administrations.", "contents": "Sex hormones metabolism in the brain: influence of central acting drugs on 5 alpha-reduction in rat diencephalon. Rats after adrenalectomy-testectomy showed a gradual increase in diencephalon 3-oxo-5 alpha-steroid: (acceptor) delta4-oxidoreductase (5 alpha-reductase) activity for 3 days. The activity then returned near to the normal range on the 4th postoperative day. When rats were given testosterone propionate (TP) 3 days after adrenalectomy-testectomy, diencephalon 5 alpha-reductase activity returned to the preoperative range 2 hr after TP administration. Diencephalon 5 alpha-reductase activity showed a highly significant increase (p less than 0.01) after a single administration of carbamazepine, reserpine, diazepam, phenytoin, phenobarbital or disulfiram. A significant increase (p less than 0.05) was also found after a single administration of methylphenidate, caffeine or methamphetamine. Plasma testosterone decreased concurrently after administration of all these agents, except diazepam. Diencephalon enzyme activity decreased significantly after repeated disulfiram administrations (p less than 0.01) but increased significantly after methamphetamine administrations (p less than 0.05). Plasma testosterone showed a tendency to decrease after repeated methamphetamine administrations but tended to increase after repeated disulfiram administrations."}
{"id": "PMID:477623", "title": "Effect of parathyroid hormone on the increase in serum glucose and insulin levels after a glucose load to thyroparathyroidectomized rats.", "content": "Thyroparathyroidectomy (TPTX) caused a significant increase in serum glucose and a corresponding fall in serum calcium in both fed and fasted rats. The increase in serum glucose, induced by TPTX, was markedly potentiated by a single intraperitoneal administration of calcium (2 mg/100 g BW) which caused a significant elevation of serum calcium in thyroparathyroidectomized rats. Parathyroid hormone (PTH; 20 U/100 g BW) administered subcutaneously to thyroparathyroidectomized rats, caused a significant decrease in serum glucose (0.1 g/100 g BW) to sham-operated rats significantly increased both serum glucose and insulin. The rise of serum glucose produced by a glucose load was markedly potentiated by TPTX, but the increase in serum insulin was not promoted significantly. The administration of PTH decreased both serum glucose and insulin levels increased by a glucose load to thyroparathyroidectomized rats, in a dose-dependent manner. The administration of calcitonin (80 MRC mU/100 g BW) significantly prevented the effect of PTH to decrease serum glucose after a glucose load to thyroparathyroidectomized rats, and calcitonin increased serum insulin. These results suggest that the effect of PTH on serum glucose does not involve insulin secretion.", "contents": "Effect of parathyroid hormone on the increase in serum glucose and insulin levels after a glucose load to thyroparathyroidectomized rats. Thyroparathyroidectomy (TPTX) caused a significant increase in serum glucose and a corresponding fall in serum calcium in both fed and fasted rats. The increase in serum glucose, induced by TPTX, was markedly potentiated by a single intraperitoneal administration of calcium (2 mg/100 g BW) which caused a significant elevation of serum calcium in thyroparathyroidectomized rats. Parathyroid hormone (PTH; 20 U/100 g BW) administered subcutaneously to thyroparathyroidectomized rats, caused a significant decrease in serum glucose (0.1 g/100 g BW) to sham-operated rats significantly increased both serum glucose and insulin. The rise of serum glucose produced by a glucose load was markedly potentiated by TPTX, but the increase in serum insulin was not promoted significantly. The administration of PTH decreased both serum glucose and insulin levels increased by a glucose load to thyroparathyroidectomized rats, in a dose-dependent manner. The administration of calcitonin (80 MRC mU/100 g BW) significantly prevented the effect of PTH to decrease serum glucose after a glucose load to thyroparathyroidectomized rats, and calcitonin increased serum insulin. These results suggest that the effect of PTH on serum glucose does not involve insulin secretion."}
{"id": "PMID:477624", "title": "Changes in fat content and some characteristics of lipolytic activity during pregnancy and lactation in mouse mammary gland.", "content": "The amount of free fatty acid in the mouse mammary gland continuously increased throughout pregnancy and lactation, while the amount of triglyceride which had been stored in the gland rapidly decreased after parturition. Higher lipolytic activity in the gland was observed in pregnancy than in nonpregnant and lactating animals. The optimum pH of the activities before and after parturition were about 6 and 7, respectively, and the activities did not decrease at high ionic strength in contrast to the ion dependent inactivation described in lipoprotein lipase. Incubation of the enzyme extract of the lactating mouse mammary gland at 50 degrees C for 10 min led to a remarkable increase in the lipolytic activity measured at pH 6.0, suggesting the existence of either an inactive form of the lipase whose optimum pH is 6.0 or some heat sensitive inhibitor(s) or inactivator(s) of the enzyme in the lactating mammary gland. The triglyceride stored in the gland in pregnancy will be consumed within the first 3rd days after parturition, and the lipases play an important role in the decomposition of the triglyceride.", "contents": "Changes in fat content and some characteristics of lipolytic activity during pregnancy and lactation in mouse mammary gland. The amount of free fatty acid in the mouse mammary gland continuously increased throughout pregnancy and lactation, while the amount of triglyceride which had been stored in the gland rapidly decreased after parturition. Higher lipolytic activity in the gland was observed in pregnancy than in nonpregnant and lactating animals. The optimum pH of the activities before and after parturition were about 6 and 7, respectively, and the activities did not decrease at high ionic strength in contrast to the ion dependent inactivation described in lipoprotein lipase. Incubation of the enzyme extract of the lactating mouse mammary gland at 50 degrees C for 10 min led to a remarkable increase in the lipolytic activity measured at pH 6.0, suggesting the existence of either an inactive form of the lipase whose optimum pH is 6.0 or some heat sensitive inhibitor(s) or inactivator(s) of the enzyme in the lactating mammary gland. The triglyceride stored in the gland in pregnancy will be consumed within the first 3rd days after parturition, and the lipases play an important role in the decomposition of the triglyceride."}
{"id": "PMID:477626", "title": "Effect of somatostatin on plasma renin activity.", "content": "Intravenous infusion of somatostatin in mongrel dogs caused a significant decrease in the peripheral plasma renin activity (PRA) enhanced by pentobarbital sodium anesthesia or furosemide treatment. However, the inhibitory activity vanished within 10 min after termination of somatostatin infusion. Intrarenal arterial infusion of somatostatin decreased furosemide-enhanced PRA in renal vein by 24.0%, 16.6% and 8.6% in dose of 0.1, 0.5 and 1.0 microgram, respectively. On the other hand, high doses of the peptide (50-200 microgram) failed to decrease. The changes in PRA occurred in the absence of any alteration in blood pressure during the intravenous infusion under furosemide treatment. In an in vitro study, the addition of somatostatin in doses of 0.01 and 0.05 microgram suppressed the renin release in dog renal cortical cell suspension by 74.3% and 53.6%, respectively. Therefore, in both intrarenal arterial infusion and the cell suspension system, somatostatin was increasingly effective in decreasing renin release towards the lower end of the dose range tested. These results suggest that the effect of somatostatin on hyperreninemia may involve an inhibition of renin release at the cell level in the kidney.", "contents": "Effect of somatostatin on plasma renin activity. Intravenous infusion of somatostatin in mongrel dogs caused a significant decrease in the peripheral plasma renin activity (PRA) enhanced by pentobarbital sodium anesthesia or furosemide treatment. However, the inhibitory activity vanished within 10 min after termination of somatostatin infusion. Intrarenal arterial infusion of somatostatin decreased furosemide-enhanced PRA in renal vein by 24.0%, 16.6% and 8.6% in dose of 0.1, 0.5 and 1.0 microgram, respectively. On the other hand, high doses of the peptide (50-200 microgram) failed to decrease. The changes in PRA occurred in the absence of any alteration in blood pressure during the intravenous infusion under furosemide treatment. In an in vitro study, the addition of somatostatin in doses of 0.01 and 0.05 microgram suppressed the renin release in dog renal cortical cell suspension by 74.3% and 53.6%, respectively. Therefore, in both intrarenal arterial infusion and the cell suspension system, somatostatin was increasingly effective in decreasing renin release towards the lower end of the dose range tested. These results suggest that the effect of somatostatin on hyperreninemia may involve an inhibition of renin release at the cell level in the kidney."}
{"id": "PMID:477627", "title": "Possible role of brain norepinephrine in the hypothalamic hypophyseal adrenal system.", "content": "Intracisternal injection of bethanidine in rats decreased brain norepinephrine turnover without affecting its endogenous level, and increased both cardiac norepinephrine turnover and serum corticosterone level. A negative correlation was observed between brain norepinephrine turnover rate and serum corticosterone level. On the other hand, when cardiac norepinephrine turnover was suppressed by intraperitoneal injection of bethanidine, serum corticosterone did not change significantly. Next, ether inhalation was added after intracisternal injection of bethanidine. Then, serum corticosterone increased more even brain norepinephrine turnover was suppressed only slightly. These data may indicate that serum corticosterone increases by selective decrease in brain norepinephrine turnover via the humoral route; from the hypothalamus down to the adrenal cortex. Inversely, intracisternal injection of corticosterone increased brain norepinephrine turnover. These results suggest that brain norepinephrine may play an inhibitory role in the tonic regulation of CRF-ACTH secretion in the higher center than the hypothalamus and there may be a closed-loop feedback system between brain norepinephrine and serum corticosterone.", "contents": "Possible role of brain norepinephrine in the hypothalamic hypophyseal adrenal system. Intracisternal injection of bethanidine in rats decreased brain norepinephrine turnover without affecting its endogenous level, and increased both cardiac norepinephrine turnover and serum corticosterone level. A negative correlation was observed between brain norepinephrine turnover rate and serum corticosterone level. On the other hand, when cardiac norepinephrine turnover was suppressed by intraperitoneal injection of bethanidine, serum corticosterone did not change significantly. Next, ether inhalation was added after intracisternal injection of bethanidine. Then, serum corticosterone increased more even brain norepinephrine turnover was suppressed only slightly. These data may indicate that serum corticosterone increases by selective decrease in brain norepinephrine turnover via the humoral route; from the hypothalamus down to the adrenal cortex. Inversely, intracisternal injection of corticosterone increased brain norepinephrine turnover. These results suggest that brain norepinephrine may play an inhibitory role in the tonic regulation of CRF-ACTH secretion in the higher center than the hypothalamus and there may be a closed-loop feedback system between brain norepinephrine and serum corticosterone."}
{"id": "PMID:477628", "title": "Effects of chlorpromazine and estradiol benzoate on prolactin secretion in gonadectomized male and female rats.", "content": "The effects of chlorpromazine (CPZ) and estradiol benzoate (EB) on serum prolactin (PRL) levels were studied in gonadectomized male and female rats. In both sexes CPZ (25 mg/kg body weight) produced an elevation of PRL when measured 2 hr after the injection, but the elevated levels were higher in ovariectomized rats than in orchidectomized rats. These results reconfirm a sexual difference in the regulatory mechanism of PRL secretion in response to the dopamine receptor blocker. Pretreatment with 5 microgram EB 48 hr before CPZ injection abolished this sexual difference in serum PRL concentration.", "contents": "Effects of chlorpromazine and estradiol benzoate on prolactin secretion in gonadectomized male and female rats. The effects of chlorpromazine (CPZ) and estradiol benzoate (EB) on serum prolactin (PRL) levels were studied in gonadectomized male and female rats. In both sexes CPZ (25 mg/kg body weight) produced an elevation of PRL when measured 2 hr after the injection, but the elevated levels were higher in ovariectomized rats than in orchidectomized rats. These results reconfirm a sexual difference in the regulatory mechanism of PRL secretion in response to the dopamine receptor blocker. Pretreatment with 5 microgram EB 48 hr before CPZ injection abolished this sexual difference in serum PRL concentration."}
{"id": "PMID:477629", "title": "Kindling phenomenon: impairment by auditory stimuli.", "content": "By coupling kindling stimuli with tones, it is shown that kindled epileptiform activity is not easily classically conditioned such that it can be evoked by an auditory stimulus. On the contrary, a tone presented 400 msec before and during the kindling stimulus impedes the rate of kindling.", "contents": "Kindling phenomenon: impairment by auditory stimuli. By coupling kindling stimuli with tones, it is shown that kindled epileptiform activity is not easily classically conditioned such that it can be evoked by an auditory stimulus. On the contrary, a tone presented 400 msec before and during the kindling stimulus impedes the rate of kindling."}
{"id": "PMID:477630", "title": "An electrophysiological analysis of the anticonvulsant action of cannabidiol on limbic seizures in conscious rats.", "content": "The effects of cannabidiol (CBD) on electrically evoked kindled seizures were studied in conscious, unrestrained rats with chronically implanted cortical and limbic electrodes, and the results were compared with those of delta 9-tetrahydrocannabinol (delta 9-THC), phenytoin (PHT), and ethosuximide (ESM). All drugs were anticonvulsant, but there were marked differences in their effects on afterdischarge (AD) threshold, duration, and amplitude. CBD, like PHT and delta 9-THC, elevated the AD threshold; in contrast, ESM decreased the threshold but suppressed AD spread. CBD, however, also resembled ESM inasmuch as both drugs decreased AD duration and amplitude. Electrophysiologically, the antiseizure effects of CBD were a combination of those of PHT and ESM. The combination of effects may account for the observation that CBD was the most efficacious of the drugs tested against limbic ADs and convulsions. Other properties of CBD were also noted: For example, compared with delta 9-THC, it is a much more selective anticonvulsant vis-\u00e0-vis motor toxicity. CBD also lacks the CNS excitatory effects produced by delta 9-THC, PHT, and ESM. These characteristics, combined with its apparently unique set of electrophysiological properties, support the suggestion that CBD has therapeutic potential as an antiepileptic.", "contents": "An electrophysiological analysis of the anticonvulsant action of cannabidiol on limbic seizures in conscious rats. The effects of cannabidiol (CBD) on electrically evoked kindled seizures were studied in conscious, unrestrained rats with chronically implanted cortical and limbic electrodes, and the results were compared with those of delta 9-tetrahydrocannabinol (delta 9-THC), phenytoin (PHT), and ethosuximide (ESM). All drugs were anticonvulsant, but there were marked differences in their effects on afterdischarge (AD) threshold, duration, and amplitude. CBD, like PHT and delta 9-THC, elevated the AD threshold; in contrast, ESM decreased the threshold but suppressed AD spread. CBD, however, also resembled ESM inasmuch as both drugs decreased AD duration and amplitude. Electrophysiologically, the antiseizure effects of CBD were a combination of those of PHT and ESM. The combination of effects may account for the observation that CBD was the most efficacious of the drugs tested against limbic ADs and convulsions. Other properties of CBD were also noted: For example, compared with delta 9-THC, it is a much more selective anticonvulsant vis-\u00e0-vis motor toxicity. CBD also lacks the CNS excitatory effects produced by delta 9-THC, PHT, and ESM. These characteristics, combined with its apparently unique set of electrophysiological properties, support the suggestion that CBD has therapeutic potential as an antiepileptic."}
{"id": "PMID:477631", "title": "Hemispheric asymmetry of pentamethylenetetrazol-induced wave-spike discharges and motor imbalance in rats.", "content": "An intrinsic asymmetry of nigrostriatal dopamine system postulated to be responsible for motor imbalance in normal rats (Glick et al., 1976) is believed to create asymmetry of arousal-sensitive wave-spike discharges. In the present study, bilaterally recorded pentamethylenetetrazol (PMZ)-activated wave-spike discharges were correlated with circling behavior in intact rats. Thirteen naive rats (10 male, 3 female) were implanted with electrodes symmetrically placed over the visual cortices, and their rotation directionality was assessed in the rotometer subsequent to administration of d-methamphetamine sul-ate, 1.5 mg/kg, i.p. In 9 rats a PMZ injection, 20 mg/kg, i.p., revealed asymmetric wave-spike bursts. All of them reliably rotated in the dirction opposite to the hemisphere with lower amplitude wave-spike discharges. It is believed that the nigrostriatal dopamine system plays a major role in modulating the asymmetry of wave-spike seizures. The findings are discussed as they relate to asymmetric generalized wave-spike dischanges found in certain petit mal patients.", "contents": "Hemispheric asymmetry of pentamethylenetetrazol-induced wave-spike discharges and motor imbalance in rats. An intrinsic asymmetry of nigrostriatal dopamine system postulated to be responsible for motor imbalance in normal rats (Glick et al., 1976) is believed to create asymmetry of arousal-sensitive wave-spike discharges. In the present study, bilaterally recorded pentamethylenetetrazol (PMZ)-activated wave-spike discharges were correlated with circling behavior in intact rats. Thirteen naive rats (10 male, 3 female) were implanted with electrodes symmetrically placed over the visual cortices, and their rotation directionality was assessed in the rotometer subsequent to administration of d-methamphetamine sul-ate, 1.5 mg/kg, i.p. In 9 rats a PMZ injection, 20 mg/kg, i.p., revealed asymmetric wave-spike bursts. All of them reliably rotated in the dirction opposite to the hemisphere with lower amplitude wave-spike discharges. It is believed that the nigrostriatal dopamine system plays a major role in modulating the asymmetry of wave-spike seizures. The findings are discussed as they relate to asymmetric generalized wave-spike dischanges found in certain petit mal patients."}
{"id": "PMID:477632", "title": "Cortical modification of an epileptogenic focus.", "content": "The influence of cerebral cortex on penicillin-induced spikes in striate cortex was studied with the technique of hypothermic block. Direct cooling of the involved or contralateral striate cortex markedly reduced seizure activity. Significant reduction in seizure activity was also seen with cooling of ipsilateral and contralateral visual association cortex. None of the other cortical areas tested had a reproducible effect on the frequency of seizures. These findings are discussed in term of local and remote factors influencing the epileptogenic focus.", "contents": "Cortical modification of an epileptogenic focus. The influence of cerebral cortex on penicillin-induced spikes in striate cortex was studied with the technique of hypothermic block. Direct cooling of the involved or contralateral striate cortex markedly reduced seizure activity. Significant reduction in seizure activity was also seen with cooling of ipsilateral and contralateral visual association cortex. None of the other cortical areas tested had a reproducible effect on the frequency of seizures. These findings are discussed in term of local and remote factors influencing the epileptogenic focus."}
{"id": "PMID:477633", "title": "Metabolism and disposition of trimethadione in pregnant rats.", "content": "The metabolism and disposition of a suspected human teratogen, trimethadione (TMO), was studied in pregnant rats following administration of the drug at doses of 60 and 240 mg/kg/day during 6 to 15 days of gestion, with a view to understanding the fetotoxicity of the drug. Following the last dose, animals were sacrificed at 6, 12, and 24 hr, and the fetuses were removed by caesarean section. The concentrations of TMO and its N-demethylated metabolite, dimethadione (DMO), were determined by a specific GLC procedure in maternal plasma, urine, brain, and liver, as well as in placenta and whole fetus. The plasma and liver concentrations of TMO and DMO suggested that the parent drug is rapidly converted to DMO. Total 24 hr urinary recoveries of the unchanged drug and the metabolite were 61 and 82% following 240 and 60 mg/kg/day doses of TMO, respectively. The DMO concentrations in brain and all other tissues analyzed were far greater than those of TMO. The fetus to maternal plasma concentration ratios of TMO suggested that the placental transfer of the drug was greater than the clearance from the fetus over the periods examined, whereas the transfer of the metablite seemed to be independent of dose. Furthermore, the rate of decline of DMO in fetus was far slower than that of the placenta and maternal plasma, causing accumulation of DMO in the fetus. The results suggest that the fetotoxic effects produced by TMO when given to pregnant rats could be due to accumulation of DMO in fetus.", "contents": "Metabolism and disposition of trimethadione in pregnant rats. The metabolism and disposition of a suspected human teratogen, trimethadione (TMO), was studied in pregnant rats following administration of the drug at doses of 60 and 240 mg/kg/day during 6 to 15 days of gestion, with a view to understanding the fetotoxicity of the drug. Following the last dose, animals were sacrificed at 6, 12, and 24 hr, and the fetuses were removed by caesarean section. The concentrations of TMO and its N-demethylated metabolite, dimethadione (DMO), were determined by a specific GLC procedure in maternal plasma, urine, brain, and liver, as well as in placenta and whole fetus. The plasma and liver concentrations of TMO and DMO suggested that the parent drug is rapidly converted to DMO. Total 24 hr urinary recoveries of the unchanged drug and the metabolite were 61 and 82% following 240 and 60 mg/kg/day doses of TMO, respectively. The DMO concentrations in brain and all other tissues analyzed were far greater than those of TMO. The fetus to maternal plasma concentration ratios of TMO suggested that the placental transfer of the drug was greater than the clearance from the fetus over the periods examined, whereas the transfer of the metablite seemed to be independent of dose. Furthermore, the rate of decline of DMO in fetus was far slower than that of the placenta and maternal plasma, causing accumulation of DMO in the fetus. The results suggest that the fetotoxic effects produced by TMO when given to pregnant rats could be due to accumulation of DMO in fetus."}
{"id": "PMID:477634", "title": "Flow-dependent salivary primidone levels in epileptic children.", "content": "In 36 epileptic children treated with primidone alone or in combination with additional anticonvulsants, salivary drug levels were compared in resting (I) and in flow-stimulated (II) saliva and were related to the corresponding serum levels. Primidone levels in saliva I and saliva II were highly correlated (r = 0.97) but were significantly (p less than 0.001) lower in saliva II; the mean difference was -38%. Serum primidone levels were highly correlated to salivary primidone levels both in saliva I (r = 0.92) and in salvia II (r = 0.91). A significant negative correlation could be established between the salivary flow rate and the saliva/serum ratio of primidone, especially in saliva I (r = 0.61; p less than 0.001). The mean saliva I/serum ratio was 1.115, reflecting drug accumulation in resting saliva. The reason primidone accumulates remains unclear. When salivary flow was stimulated, the mean saliva/serum ratio decreased to 0.7, indicating the development of a drug concentration slope from blood to saliva. This is explained by the limited permeation of the drug through cellular membranes due to its rather low lipid solubility. From the data it can be concluded that saliva is suitable for monitoring primidone levels provided the conditions of sample collection are standardized.", "contents": "Flow-dependent salivary primidone levels in epileptic children. In 36 epileptic children treated with primidone alone or in combination with additional anticonvulsants, salivary drug levels were compared in resting (I) and in flow-stimulated (II) saliva and were related to the corresponding serum levels. Primidone levels in saliva I and saliva II were highly correlated (r = 0.97) but were significantly (p less than 0.001) lower in saliva II; the mean difference was -38%. Serum primidone levels were highly correlated to salivary primidone levels both in saliva I (r = 0.92) and in salvia II (r = 0.91). A significant negative correlation could be established between the salivary flow rate and the saliva/serum ratio of primidone, especially in saliva I (r = 0.61; p less than 0.001). The mean saliva I/serum ratio was 1.115, reflecting drug accumulation in resting saliva. The reason primidone accumulates remains unclear. When salivary flow was stimulated, the mean saliva/serum ratio decreased to 0.7, indicating the development of a drug concentration slope from blood to saliva. This is explained by the limited permeation of the drug through cellular membranes due to its rather low lipid solubility. From the data it can be concluded that saliva is suitable for monitoring primidone levels provided the conditions of sample collection are standardized."}
{"id": "PMID:477637", "title": "The use of althesin in drug-resistent status epilepticus.", "content": "The properties of Althesin (anticonvulsant activity, depression of oxygen consumption, lowering of ICP, rapid excretion) led us to use this steroid combination to treat 11 patients in status epilepticus resistant to the standard drugs (benzodiazepines and barbiturates). The administration of Althesin by slow intravenous injection was ineffective in 2 of the 3 patients thus treated. The doses used (2--10 ml) were probably too small. One only administration of a 10% solution of Althesin in 10% fructose by intravenous drip (the rate was calculated so as to obtain the burst suppression stage at the EEG) stopped status epilepticus in 7 of the 9 patients thus treated. In this group the doses used varied from 25 to 50 ml. The 2 patients in whom it was necessary to repeat Althesin administration and combine it with other drugs had both been operated on for severe brain injuries involving marked cerebral edema. In spite of the very small number of cases, the definitive arrest of status epilepticus obtained in 8 out of 11 patients first treated with other drugs is encouraging: Althesin probably may be regarded as an adjunct in the treatment of status epilepticus.", "contents": "The use of althesin in drug-resistent status epilepticus. The properties of Althesin (anticonvulsant activity, depression of oxygen consumption, lowering of ICP, rapid excretion) led us to use this steroid combination to treat 11 patients in status epilepticus resistant to the standard drugs (benzodiazepines and barbiturates). The administration of Althesin by slow intravenous injection was ineffective in 2 of the 3 patients thus treated. The doses used (2--10 ml) were probably too small. One only administration of a 10% solution of Althesin in 10% fructose by intravenous drip (the rate was calculated so as to obtain the burst suppression stage at the EEG) stopped status epilepticus in 7 of the 9 patients thus treated. In this group the doses used varied from 25 to 50 ml. The 2 patients in whom it was necessary to repeat Althesin administration and combine it with other drugs had both been operated on for severe brain injuries involving marked cerebral edema. In spite of the very small number of cases, the definitive arrest of status epilepticus obtained in 8 out of 11 patients first treated with other drugs is encouraging: Althesin probably may be regarded as an adjunct in the treatment of status epilepticus."}
{"id": "PMID:477638", "title": "Causes of death in institutionalized epileptics.", "content": "To assess the causes of death in institutionalized epileptics, the patient records, death certificates, and other data from the only hospital for epileptics in Finland (Vaajasalo Hospital) were reexamined. During the years 1900--1976, 179 inpatients in Vaajasalo Hospital died; this was 12% of all inpatients. The most common causes of death were as follows: pneumonia in 40 cases, seizures in 34 cases (single seizure in 18 and status epileptics in 16), drowning in 29 cases, stroke in 10 cases, and heart infarct in 9 cases. Chronic intoxication caused by phenytoin and/or phenobarbitol was a common supplementary factor leading to death in patients who died of pneumonia or seizures. Thirteen deaths were recorded as suicides or suspected suicides (11 by drowning and 2 by strangulation). The results reflect the severe epilepsy and the poor conditions of the patients, as well as the poor condition of the local facilities in the past. The information obtained should be useful in the effort to improve medical care for these patients.", "contents": "Causes of death in institutionalized epileptics. To assess the causes of death in institutionalized epileptics, the patient records, death certificates, and other data from the only hospital for epileptics in Finland (Vaajasalo Hospital) were reexamined. During the years 1900--1976, 179 inpatients in Vaajasalo Hospital died; this was 12% of all inpatients. The most common causes of death were as follows: pneumonia in 40 cases, seizures in 34 cases (single seizure in 18 and status epileptics in 16), drowning in 29 cases, stroke in 10 cases, and heart infarct in 9 cases. Chronic intoxication caused by phenytoin and/or phenobarbitol was a common supplementary factor leading to death in patients who died of pneumonia or seizures. Thirteen deaths were recorded as suicides or suspected suicides (11 by drowning and 2 by strangulation). The results reflect the severe epilepsy and the poor conditions of the patients, as well as the poor condition of the local facilities in the past. The information obtained should be useful in the effort to improve medical care for these patients."}
{"id": "PMID:477639", "title": "Hemisphere-dependent cognitive performances in epileptic patients.", "content": "Short-term memory (STM), long-term memory (LTM), and recency judgment were investigated in 75 right-handed nonaphasic patients without visual deficits who had epileptic foci localized in one of four different regions of the brain: right temporal lobe, left temporal lobe, right frontal lobe, and left frontal lobe. The results led to the following conclusions: (1) left temporal lesions yielded comparatively greater impairment for the verbal LTM task, whereas right temporal lesions were more disruptive for the spatial LTM task; (2) no significant differences between left- and right-brain-damaged patients in STM tasks were obtained; (3) left frontal lesions yielded deficits in a recency task in accordance with hemispheric specialization; (4) only prolonged epileptic syndromes impaired neuropsychological performance in those tests; (5) comparisons between male and female patients and between those with and without clear evidence of a structural lesion did not reach statistical significance.", "contents": "Hemisphere-dependent cognitive performances in epileptic patients. Short-term memory (STM), long-term memory (LTM), and recency judgment were investigated in 75 right-handed nonaphasic patients without visual deficits who had epileptic foci localized in one of four different regions of the brain: right temporal lobe, left temporal lobe, right frontal lobe, and left frontal lobe. The results led to the following conclusions: (1) left temporal lesions yielded comparatively greater impairment for the verbal LTM task, whereas right temporal lesions were more disruptive for the spatial LTM task; (2) no significant differences between left- and right-brain-damaged patients in STM tasks were obtained; (3) left frontal lesions yielded deficits in a recency task in accordance with hemispheric specialization; (4) only prolonged epileptic syndromes impaired neuropsychological performance in those tests; (5) comparisons between male and female patients and between those with and without clear evidence of a structural lesion did not reach statistical significance."}
{"id": "PMID:477640", "title": "Distinction between normal and epileptic rhythms in rodent sensorimotor cortex.", "content": "Characteristics of spontaneous spindling in electrocorticograms from awake rodents are described. Comparisons are made between this spontaneous activity and epileptic activity generated in the sensorimotor cortex.", "contents": "Distinction between normal and epileptic rhythms in rodent sensorimotor cortex. Characteristics of spontaneous spindling in electrocorticograms from awake rodents are described. Comparisons are made between this spontaneous activity and epileptic activity generated in the sensorimotor cortex."}
{"id": "PMID:477641", "title": "Do anticonvulsants reduce the efficacy of oral contraceptives?", "content": "Women in our area with epilepsy who were also taking oral contraceptives were identified. Of 82 patients taking oral contraceptives, 41 had used both anticonvulsants and oral contraceptives for a total of 955 months. Three documented oral contraceptive (pill) failures occurred during this period, whereas the expected number of 0.12 (relative risk, 25; 95% confience interval, 5 to 73). No pill failures were observed in 2,278 months among women with epilepsy who were taking oral contraceptives but who were not taking anticonvulsants at this time. Thus our data support the suggestion that there is an increased rate of pill failure among women taking anticonvulsants. In view of this diminished effectiveness, the advisability of using oral contraceptives rather than one of the other forms of contraception when anticonvulsant medication is being used concurrently may need to be reevaluated.", "contents": "Do anticonvulsants reduce the efficacy of oral contraceptives? Women in our area with epilepsy who were also taking oral contraceptives were identified. Of 82 patients taking oral contraceptives, 41 had used both anticonvulsants and oral contraceptives for a total of 955 months. Three documented oral contraceptive (pill) failures occurred during this period, whereas the expected number of 0.12 (relative risk, 25; 95% confience interval, 5 to 73). No pill failures were observed in 2,278 months among women with epilepsy who were taking oral contraceptives but who were not taking anticonvulsants at this time. Thus our data support the suggestion that there is an increased rate of pill failure among women taking anticonvulsants. In view of this diminished effectiveness, the advisability of using oral contraceptives rather than one of the other forms of contraception when anticonvulsant medication is being used concurrently may need to be reevaluated."}
{"id": "PMID:477642", "title": "Ictal body scheme disturbance induced by looking through a small opening.", "content": "The case of a patient is reported in which seizures consisting of a feeling that the left arm was absent were induced by looking through a small opening (e.g. camera view finder). The EEG showed right parietal sharp wave discharges between and during such seizures. This observation is interpreted as an example of a reflex epilepsy triggered by the specific stimulus of looking through a small opening, and involving the right parietal lobe.", "contents": "Ictal body scheme disturbance induced by looking through a small opening. The case of a patient is reported in which seizures consisting of a feeling that the left arm was absent were induced by looking through a small opening (e.g. camera view finder). The EEG showed right parietal sharp wave discharges between and during such seizures. This observation is interpreted as an example of a reflex epilepsy triggered by the specific stimulus of looking through a small opening, and involving the right parietal lobe."}
{"id": "PMID:477643", "title": "Pseudohypoparathyroidism and epilepsy: diagnostic value of computerized cranial tomography.", "content": "Computerized cranial tomograms (CCTs) unexpectedly showed bilateral symmetrical calcifications in the basal ganglia and frontal areas in two unrelated epileptic patients 12 and 13 years of age. The patients presented with a variety of seizures, some with focal features; these seizures were resistant to medication in the first case. Subsequent testing revealed hypocalcemia and other biochemical and radiologic features of pseudohypoparathyroidism, despite absence of the usual phenotypic features, tetanic symptoms, and positive family history. The CCT scan may provide the first clue to an underlying hypocalcemic disorder in an epileptic patient even when the skull X-ray is normal. Early detection of this metabolic condition by CCT scanning allows specific treatment to restore serum calcium levels to normal, which usually eliminates seizures and favors optimal cerebral functioning. Serial CCT scanning also provides a useful means for following the intracerebral calcifications, which remained unchanged after 1 and 2 years of normocalcemia in our 2 patients.", "contents": "Pseudohypoparathyroidism and epilepsy: diagnostic value of computerized cranial tomography. Computerized cranial tomograms (CCTs) unexpectedly showed bilateral symmetrical calcifications in the basal ganglia and frontal areas in two unrelated epileptic patients 12 and 13 years of age. The patients presented with a variety of seizures, some with focal features; these seizures were resistant to medication in the first case. Subsequent testing revealed hypocalcemia and other biochemical and radiologic features of pseudohypoparathyroidism, despite absence of the usual phenotypic features, tetanic symptoms, and positive family history. The CCT scan may provide the first clue to an underlying hypocalcemic disorder in an epileptic patient even when the skull X-ray is normal. Early detection of this metabolic condition by CCT scanning allows specific treatment to restore serum calcium levels to normal, which usually eliminates seizures and favors optimal cerebral functioning. Serial CCT scanning also provides a useful means for following the intracerebral calcifications, which remained unchanged after 1 and 2 years of normocalcemia in our 2 patients."}
{"id": "PMID:477644", "title": "Effectiveness of clonazepan in startle-induced seizures.", "content": "Five cerebral-palsied children and adolescents with severe startle epilepsy became seizure-free after clonazepan was introduced into their existing anticonvulsant drug regimens. The drug was withdrawn in one case because of side effects. Two hemiparetic patients who had startle epilepsy as the only epileptic manifestation remained permanently controlled after a mean of 34 months of continuous therapy. Reappearance of startle-induced seizures occurred after 1 and 4 years in two other patients with the Lennox-Gastaut syndrome. A possible explanation for the effectiveness of clonazepan in this form of reflex epilepsy may involve inhibition of brainstem mechanisms mediating pathologically enhanced reactions in these patients, thus avoiding secondary activation of a discharging focus in the vicinity of the motor supplementary area.", "contents": "Effectiveness of clonazepan in startle-induced seizures. Five cerebral-palsied children and adolescents with severe startle epilepsy became seizure-free after clonazepan was introduced into their existing anticonvulsant drug regimens. The drug was withdrawn in one case because of side effects. Two hemiparetic patients who had startle epilepsy as the only epileptic manifestation remained permanently controlled after a mean of 34 months of continuous therapy. Reappearance of startle-induced seizures occurred after 1 and 4 years in two other patients with the Lennox-Gastaut syndrome. A possible explanation for the effectiveness of clonazepan in this form of reflex epilepsy may involve inhibition of brainstem mechanisms mediating pathologically enhanced reactions in these patients, thus avoiding secondary activation of a discharging focus in the vicinity of the motor supplementary area."}
{"id": "PMID:477646", "title": "The use of ionising radiation for the treatment of injuries to flexor tendons and supporting ligaments in horses.", "content": "A technique was developed using radioactive isotopes as a source of radiation for the treatment of injuries to the superficial and deep flexor tendons and the associated ligaments in the horse. The treatment area was sub-divided so that different dosages could be applied over the limb as necessary. A plaster of Paris impression was taken of the whole area to be treated. In the isotope laboratory a plaster negative was made and loaded with the dose of radioactive isotope. The loaded cast was then strapped to the horse's limb for the calculated time, usually about three days. A total of 42 horses were treated and follow up information was obtained from 28. Twenty-five animals raced again; two relapsed before racing and one was destroyed with navicular disease. Ten of the 42 horses had been treated by firing before irradiation. Five of these returned to racing but the history of four of them was not known.", "contents": "The use of ionising radiation for the treatment of injuries to flexor tendons and supporting ligaments in horses. A technique was developed using radioactive isotopes as a source of radiation for the treatment of injuries to the superficial and deep flexor tendons and the associated ligaments in the horse. The treatment area was sub-divided so that different dosages could be applied over the limb as necessary. A plaster of Paris impression was taken of the whole area to be treated. In the isotope laboratory a plaster negative was made and loaded with the dose of radioactive isotope. The loaded cast was then strapped to the horse's limb for the calculated time, usually about three days. A total of 42 horses were treated and follow up information was obtained from 28. Twenty-five animals raced again; two relapsed before racing and one was destroyed with navicular disease. Ten of the 42 horses had been treated by firing before irradiation. Five of these returned to racing but the history of four of them was not known."}
{"id": "PMID:477648", "title": "Oxygenation of pulmonary vein blood in conscious and anaesthetised ponies.", "content": "Catheters were implanted through the left atrium into large veins drawing blood from regions of the diaphragmatic lobes in five ponies. Measurement of oxygen and carbon dioxide tensions in blood samples drawn through these catheters showed that in laterally recumbent conscious and anaesthetised animals the function of the lowermost lung is progressively impaired while in the supine position the function of both lungs is adversely affected.", "contents": "Oxygenation of pulmonary vein blood in conscious and anaesthetised ponies. Catheters were implanted through the left atrium into large veins drawing blood from regions of the diaphragmatic lobes in five ponies. Measurement of oxygen and carbon dioxide tensions in blood samples drawn through these catheters showed that in laterally recumbent conscious and anaesthetised animals the function of the lowermost lung is progressively impaired while in the supine position the function of both lungs is adversely affected."}
{"id": "PMID:477649", "title": "Enteroliths in horses--a retrospective study of 30 cases.", "content": "In a retrospective study of selected cases, abdominal colic in 30 horses was attributed to enterolith obstructions of the large intestine. Obstructions caused by \"true\" enteroliths were confined to horses more than four years old. Prominent clinical features were recurrent mild abdominal pain, inappetance, gaseous distension and minimal intestinal motility. The various aspects of the clinical syndrome, including diagnostic problems and clinical management, are discussed. Most obstructing enteroliths were found near the beginning of the small colon and most horses contained only a single major concretion. Enteroliths were formed by mineral deposition in concentric layers about a central nidus of ingested material and were spherical or tetrahedral in shape. Intestinal concretions were found to consist primarily of ammonium magnesium phosphate.", "contents": "Enteroliths in horses--a retrospective study of 30 cases. In a retrospective study of selected cases, abdominal colic in 30 horses was attributed to enterolith obstructions of the large intestine. Obstructions caused by \"true\" enteroliths were confined to horses more than four years old. Prominent clinical features were recurrent mild abdominal pain, inappetance, gaseous distension and minimal intestinal motility. The various aspects of the clinical syndrome, including diagnostic problems and clinical management, are discussed. Most obstructing enteroliths were found near the beginning of the small colon and most horses contained only a single major concretion. Enteroliths were formed by mineral deposition in concentric layers about a central nidus of ingested material and were spherical or tetrahedral in shape. Intestinal concretions were found to consist primarily of ammonium magnesium phosphate."}
{"id": "PMID:477650", "title": "Staphylococcal septic arthritis in three horses.", "content": "Three horses were diagnosed as having monarticular septic arthritis due to Staphylococcus aureus on the basis of culture of articular cartilage, synovial membrane and/or synovial fluid. The organisms were all well recognised human phage types and in two cases demonstrated beta-lactamase (penicillinase) activity. Details of case histories are presented and the bacteriological techniques and antibiotic management with cloxacillin, methicillin and penicillin discussed. Following treatment, sterile cultures of synovial fluid were achieved in all cases, but in two horses the infections resulted in degenerative articular changes. This necessitated arthrodesis of the fetlock joint in one case.", "contents": "Staphylococcal septic arthritis in three horses. Three horses were diagnosed as having monarticular septic arthritis due to Staphylococcus aureus on the basis of culture of articular cartilage, synovial membrane and/or synovial fluid. The organisms were all well recognised human phage types and in two cases demonstrated beta-lactamase (penicillinase) activity. Details of case histories are presented and the bacteriological techniques and antibiotic management with cloxacillin, methicillin and penicillin discussed. Following treatment, sterile cultures of synovial fluid were achieved in all cases, but in two horses the infections resulted in degenerative articular changes. This necessitated arthrodesis of the fetlock joint in one case."}
{"id": "PMID:477651", "title": "A case of carpal intersynovial fistula in a horse.", "content": "A tentative diagnosis of a fistula between the tendon sheaths of the right extensor carpi radialis and the intercarpal joint was confirmed by positive contrast radiography following injection of sodium diatrizoate into the joint. Surgical removal of a tag of synovial membrane and repair of the fistula was carried out. Post surgical treatment included mild exercise to prevent adhesions within the sheath. A month following discharge, contrast radiography revealed no communication between joint and tendon sheath. At this time mild distension of the sheath was relieved by aspiration and it did not recur when the horse returned to work following a further month of rest.", "contents": "A case of carpal intersynovial fistula in a horse. A tentative diagnosis of a fistula between the tendon sheaths of the right extensor carpi radialis and the intercarpal joint was confirmed by positive contrast radiography following injection of sodium diatrizoate into the joint. Surgical removal of a tag of synovial membrane and repair of the fistula was carried out. Post surgical treatment included mild exercise to prevent adhesions within the sheath. A month following discharge, contrast radiography revealed no communication between joint and tendon sheath. At this time mild distension of the sheath was relieved by aspiration and it did not recur when the horse returned to work following a further month of rest."}
{"id": "PMID:477658", "title": "Purification and some properties of a hitherto-unknown enzyme reducing the carbon-carbon double bond of alpha, beta-unsaturated carboxylate anions.", "content": "2-Enoate-reductase, a previously unknown soluble enzyme is present in Clostridium kluyveri and another Clostridium species growing on (E)-2-butenoate. From the latter the reductase was purified 88-fold with an overall yield up to 74%. The enzyme was pure as judged by polyacrylamide gel electrophoresis with and without sodium dodecyl sulphate as well as by isoelectric focusing. The purification of the enzyme was performed in the presence of (E)-2-methyl-2-butenoate as substrate to keep the enzyme in the oxidized state and under anaerobic conditions. The purification procedure included an ammonium sulphate precipitation, chromatography on DEAE-Sepharose CL-6B, hydroxylapatite and Sepharose CL-6B. The enzyme reduces different alpha,beta-unsaturated carboxylate anions such as (E)-2-butenoate, (E)-2-methyl-2-butenoate, (E)-cinnamate and probably many others in a NADH-dependent reaction to the saturated carboxylate anions. Fumarate, 3-phenyl-2-propinate, 2-enoyl-methyl and CoA esters proved not to be substrates for the purified reductase. NADPH does not act as an electron donor. The enzyme was shown to have a molecular weight of about 450,000 by gel chromatography. It consists of subunits with a molecular weight of 78,000. Per subunit about 1 FAD, 3.5--3.8 atoms of iron and 4.0 labile sulphur atoms have been found indicating a conjugated iron-sulphur flavo-protein. Copper could not be detected. The isoelectric point was 8.4. As shown by absorption spectroscopy the enzyme can be reduced by NADH and reoxidized with dichloroindophenol, hexacyanoferrate III, oxygen and substrates. Addition of 8 mol p-hydroxymercuribenzoate to 1 mol subunit completely destroyed the activity of the reductase. So far no physiological role of the enzyme is known.", "contents": "Purification and some properties of a hitherto-unknown enzyme reducing the carbon-carbon double bond of alpha, beta-unsaturated carboxylate anions. 2-Enoate-reductase, a previously unknown soluble enzyme is present in Clostridium kluyveri and another Clostridium species growing on (E)-2-butenoate. From the latter the reductase was purified 88-fold with an overall yield up to 74%. The enzyme was pure as judged by polyacrylamide gel electrophoresis with and without sodium dodecyl sulphate as well as by isoelectric focusing. The purification of the enzyme was performed in the presence of (E)-2-methyl-2-butenoate as substrate to keep the enzyme in the oxidized state and under anaerobic conditions. The purification procedure included an ammonium sulphate precipitation, chromatography on DEAE-Sepharose CL-6B, hydroxylapatite and Sepharose CL-6B. The enzyme reduces different alpha,beta-unsaturated carboxylate anions such as (E)-2-butenoate, (E)-2-methyl-2-butenoate, (E)-cinnamate and probably many others in a NADH-dependent reaction to the saturated carboxylate anions. Fumarate, 3-phenyl-2-propinate, 2-enoyl-methyl and CoA esters proved not to be substrates for the purified reductase. NADPH does not act as an electron donor. The enzyme was shown to have a molecular weight of about 450,000 by gel chromatography. It consists of subunits with a molecular weight of 78,000. Per subunit about 1 FAD, 3.5--3.8 atoms of iron and 4.0 labile sulphur atoms have been found indicating a conjugated iron-sulphur flavo-protein. Copper could not be detected. The isoelectric point was 8.4. As shown by absorption spectroscopy the enzyme can be reduced by NADH and reoxidized with dichloroindophenol, hexacyanoferrate III, oxygen and substrates. Addition of 8 mol p-hydroxymercuribenzoate to 1 mol subunit completely destroyed the activity of the reductase. So far no physiological role of the enzyme is known."}
{"id": "PMID:477660", "title": "Hydrodynamic parameters of the detergent-solubilised hydrogenase from Paracoccus denitrificans.", "content": "The hydrogenase from Paracoccus denitrificans, which is an intrinsic membrane protein, has been solubilised from membranes by Triton X-100. The partial specific volume of the solubilised protein has been determined using sucrose density gradient centrifugation in H2O and 2H2O. The values of the specific volumes of hydrogenase, measured in the presence or absence of Triton X-100, are 0.73 and 0.74 ml . g-1, respectively, indicating that hydrogenase binds much less than one micelle of Triton X-100. The sedimentation coefficient of hydrogenase is increased from 10.4 S to 15.9 S on removal of detergent. The Stokes' radius of hydrogenase, determined by gel filtration on Sepharose 6B, is 5.5 nm in the presence of Triton X-100 compared to 6.7 nm in the absence of detergent. The apparent molecular weight therefore increases from 242,500 to 466,000 on removal of detergent. In the presence of urea and sodium dodecylsulphate, the hydrogenase has an apparent molecular weight of 63,000. The enzyme therefore behaves as a non-covalently linked tetramer in the presence of Triton X-100. Removal of Triton X-100 results in association of tetramers to form octamers.", "contents": "Hydrodynamic parameters of the detergent-solubilised hydrogenase from Paracoccus denitrificans. The hydrogenase from Paracoccus denitrificans, which is an intrinsic membrane protein, has been solubilised from membranes by Triton X-100. The partial specific volume of the solubilised protein has been determined using sucrose density gradient centrifugation in H2O and 2H2O. The values of the specific volumes of hydrogenase, measured in the presence or absence of Triton X-100, are 0.73 and 0.74 ml . g-1, respectively, indicating that hydrogenase binds much less than one micelle of Triton X-100. The sedimentation coefficient of hydrogenase is increased from 10.4 S to 15.9 S on removal of detergent. The Stokes' radius of hydrogenase, determined by gel filtration on Sepharose 6B, is 5.5 nm in the presence of Triton X-100 compared to 6.7 nm in the absence of detergent. The apparent molecular weight therefore increases from 242,500 to 466,000 on removal of detergent. In the presence of urea and sodium dodecylsulphate, the hydrogenase has an apparent molecular weight of 63,000. The enzyme therefore behaves as a non-covalently linked tetramer in the presence of Triton X-100. Removal of Triton X-100 results in association of tetramers to form octamers."}
{"id": "PMID:477662", "title": "The kinetics of ATP-dependent exonuclease V from Micrococcus lysodeikticus. A Michaelian dependence on DNA concentration.", "content": "The ATP-dependent exonuclease V from Micrococcus lysodekticus shows a Michaelian relation between steady-state velocity and the concentration of T7 DNA substrate. The Km (expressed as a mass concentration) does not change when the T7 DNA is broken into smaller fragments by a restriction enzyme. This is interpreted to mean that the predominant process by which the exonuclease-V--DNA complex breaks down is digestion of the entire DNA molecule rather than physical dissociation, in accord with the already known processive nature of degradation by this enzyme. The way that the V and Km towards DNA vary with ATP and ADP concentration suggests that enzyme-DNA complex is predominantly formed by reaction of DNA with an enzyme-ATP complex rather than with bare enzyme.", "contents": "The kinetics of ATP-dependent exonuclease V from Micrococcus lysodeikticus. A Michaelian dependence on DNA concentration. The ATP-dependent exonuclease V from Micrococcus lysodekticus shows a Michaelian relation between steady-state velocity and the concentration of T7 DNA substrate. The Km (expressed as a mass concentration) does not change when the T7 DNA is broken into smaller fragments by a restriction enzyme. This is interpreted to mean that the predominant process by which the exonuclease-V--DNA complex breaks down is digestion of the entire DNA molecule rather than physical dissociation, in accord with the already known processive nature of degradation by this enzyme. The way that the V and Km towards DNA vary with ATP and ADP concentration suggests that enzyme-DNA complex is predominantly formed by reaction of DNA with an enzyme-ATP complex rather than with bare enzyme."}
{"id": "PMID:477663", "title": "Regulation of glycogen synthesis in rat-hepatocyte cultures by glucose, insulin and glucocorticoids.", "content": "The changes in glycogen content and in its rate of synthesis in two-day-old primary cultures of rat hepatocytes were assessed under various conditions. Hepatocytes cultivated in serum-free and hormone-free medium switch from glycogen degradation to glycogen deposition at 10.3 mM glucose. After pretreatment of the cells with glucocorticoids this threshold was reduced, in the absence or presence of insulin, to 5.4 or 1.2 mM glucose, respectively. The rate of glycogen synthesis in the presence of 10 mM glucose was amplified from 5 nmol x h-1 x mg protein-1 to 20 nmol glucose x h-1 x mg protein-1 after pretreatment with triamcinolone. Glucagon pretreatment also significantly increased the subsequent glycogen synthesis rate. Insulin addition accelerated glycogen synthesis about twofold regardless of the pretreatment. The dose-response relationship between insulin concentration and glycogen synthesis rate showed half-maximal effect at 0.62 +/- 0.22 nM (mean +/- S.D.) insulin. Pretreatment of hepatocytes with glucocorticoids, glucagon, insulin or combinations of these hormones did not significantly change the concentration which gives the half-maximal effect.", "contents": "Regulation of glycogen synthesis in rat-hepatocyte cultures by glucose, insulin and glucocorticoids. The changes in glycogen content and in its rate of synthesis in two-day-old primary cultures of rat hepatocytes were assessed under various conditions. Hepatocytes cultivated in serum-free and hormone-free medium switch from glycogen degradation to glycogen deposition at 10.3 mM glucose. After pretreatment of the cells with glucocorticoids this threshold was reduced, in the absence or presence of insulin, to 5.4 or 1.2 mM glucose, respectively. The rate of glycogen synthesis in the presence of 10 mM glucose was amplified from 5 nmol x h-1 x mg protein-1 to 20 nmol glucose x h-1 x mg protein-1 after pretreatment with triamcinolone. Glucagon pretreatment also significantly increased the subsequent glycogen synthesis rate. Insulin addition accelerated glycogen synthesis about twofold regardless of the pretreatment. The dose-response relationship between insulin concentration and glycogen synthesis rate showed half-maximal effect at 0.62 +/- 0.22 nM (mean +/- S.D.) insulin. Pretreatment of hepatocytes with glucocorticoids, glucagon, insulin or combinations of these hormones did not significantly change the concentration which gives the half-maximal effect."}
{"id": "PMID:477664", "title": "Trans-membrane translocation of proteins. The direct transfer model.", "content": "As a start towards a deeper understanding of the transmembrane transport of proteins, the transfer of a nascent chain through the lipophilic core of a membrane is discussed from a physico-chemical point of view. Some simple considerations of the energetics of protein structure, together with experimental data on the transfer process, form the basis for a detailed and quantifiable model, accounting for the extrusion of secreted proteins as well as for the insertion of trans-membrane proteins.", "contents": "Trans-membrane translocation of proteins. The direct transfer model. As a start towards a deeper understanding of the transmembrane transport of proteins, the transfer of a nascent chain through the lipophilic core of a membrane is discussed from a physico-chemical point of view. Some simple considerations of the energetics of protein structure, together with experimental data on the transfer process, form the basis for a detailed and quantifiable model, accounting for the extrusion of secreted proteins as well as for the insertion of trans-membrane proteins."}
{"id": "PMID:477666", "title": "Partial purification and characterization of the proteins from the 40-S ribosomes of Artemia salina and wheat germ.", "content": "The proteins were extracted from purified 40-S ribosomes derived from wheat germ and Artemia salina and separated by carboxymethylcellulose ion-exchange chromatography. Approximately four proteins from Artemia and four proteins from wheat germ were separated in a state of high purity. All proteins were identified by co-electrophoresis using a two-dimensional polyacrylamide gel system. A total of 30 unique proteins were found for Artemia and 32 proteins for wheat. The molecular weights of all proteins were estimated by sodium dodecylsulfate gel electrophoresis. Assuming each protein to be present in one copy per 40-S ribosome, the total protein molecular weight was estimated to be 560,000 associated with Artemia 40-S particles and 550,000 associated with wheat germ 40-S ribosomes.", "contents": "Partial purification and characterization of the proteins from the 40-S ribosomes of Artemia salina and wheat germ. The proteins were extracted from purified 40-S ribosomes derived from wheat germ and Artemia salina and separated by carboxymethylcellulose ion-exchange chromatography. Approximately four proteins from Artemia and four proteins from wheat germ were separated in a state of high purity. All proteins were identified by co-electrophoresis using a two-dimensional polyacrylamide gel system. A total of 30 unique proteins were found for Artemia and 32 proteins for wheat. The molecular weights of all proteins were estimated by sodium dodecylsulfate gel electrophoresis. Assuming each protein to be present in one copy per 40-S ribosome, the total protein molecular weight was estimated to be 560,000 associated with Artemia 40-S particles and 550,000 associated with wheat germ 40-S ribosomes."}
{"id": "PMID:477667", "title": "Effect of sulphated glycopeptides on kinetics of 3-O-methyl glucose and 2-deoxyglucose transport by epithelial cells isolated from rabbit small intestine.", "content": "Suspensions of cells isolated from rabbit small intestine were prepared which showed a considerable rate of respiration after 4 h of their isolation. Rabbit intestinal cells accumulated 3-O-methyl-glucose by an active transport mechanism which was Na+-dependent whereas 2-deoxyglucose was transported by a saturable pathway. Analysis of the initial transport data indicated that the uptake of both sugars by cells followed Michaelis-Menten-type kinetics. These cells also showed a considerable degree of 2-deoxyglucose phosphorylation and the transport of the sugar into the cells appeared to be a rate-limiting factor for its phosphorylation. Sulphated glycopeptide had no effect on the respiration rate of these cells but it increased the transport of 3-O-methyl-glucose and 2-deoxyglucose into cells by many fold compared with untreated cells. Kinetic analysis of the data indicated that the increase in the transport was due to increased V while the Km remained unchanged. Presence of sulphated glycopeptide had no effect on the kinetics of phosphorylation of 2-deoxyglucose. These effects of sulphated glycopeptide on the transport activity of the cells have been discussed as a result of an increase in the number of sugar sites in the cell membrane due to unmasking or activation of preexisting sites owing to the interaction between the cell membrane components and highly polyanionic sulphated glycopeptides.", "contents": "Effect of sulphated glycopeptides on kinetics of 3-O-methyl glucose and 2-deoxyglucose transport by epithelial cells isolated from rabbit small intestine. Suspensions of cells isolated from rabbit small intestine were prepared which showed a considerable rate of respiration after 4 h of their isolation. Rabbit intestinal cells accumulated 3-O-methyl-glucose by an active transport mechanism which was Na+-dependent whereas 2-deoxyglucose was transported by a saturable pathway. Analysis of the initial transport data indicated that the uptake of both sugars by cells followed Michaelis-Menten-type kinetics. These cells also showed a considerable degree of 2-deoxyglucose phosphorylation and the transport of the sugar into the cells appeared to be a rate-limiting factor for its phosphorylation. Sulphated glycopeptide had no effect on the respiration rate of these cells but it increased the transport of 3-O-methyl-glucose and 2-deoxyglucose into cells by many fold compared with untreated cells. Kinetic analysis of the data indicated that the increase in the transport was due to increased V while the Km remained unchanged. Presence of sulphated glycopeptide had no effect on the kinetics of phosphorylation of 2-deoxyglucose. These effects of sulphated glycopeptide on the transport activity of the cells have been discussed as a result of an increase in the number of sugar sites in the cell membrane due to unmasking or activation of preexisting sites owing to the interaction between the cell membrane components and highly polyanionic sulphated glycopeptides."}
{"id": "PMID:477668", "title": "N-Acetylaminoacyl-p-nitranilidase from human placenta. Purification and some properties.", "content": "An enzyme hydrolyzing N-acetylaminoacyl-p-nitroanilides has been isolated from mature human placentae by a six-step procedure comprising extraction from a placenta homogenate, ammonium sulfate fractionation, treatment with isopentyl alcohol, chromatography on CM-Sephadex, protamine sulfate precipitation and gel filtration on an Ultrogel AcA 34 column. About 2500-fold enrichement was achieved from placenta homogenate. The purified enzyme preparation showed a single band on polyacrylamide disc electrophoresis. The molecular weight was estimated to be 380,000 by gel filtration. Placental extracts contain two main isoenzymes of pI 3.9 and 4.5 respectively. Activity was strongly inhibited by chloromercuribenzoate, slightly inhibited by Ca2+ and cysteine; no activation could be detected. The enzyme exhibits an exopeptidase-like activity towards acetyl-dipeptides with a certain specifity towards N-acetylalanyl-alanine; N-acetylalanine-p-nitroanilide, however, is hydrolyzed four times faster. With N-acetylalanine-p-nitroanilide as substrate the pH optimum was 8.0--8.2; Km was 2.13 mmol/l. N-Acetylleucine-p-nitroanilide and N-acetyltyrosine-p-nitroanilide were split slowly; N-acetylalanyl-alanyl-alanine-p-nitroanilide, N-butyloxycarbonyl-alanyl-alanine-p-nitroanilide, unsubstituted analogous aminoacyl-p-nitroanilides and several protein substrates were not hydrolyzed. The functions of the enzyme are still unknown.", "contents": "N-Acetylaminoacyl-p-nitranilidase from human placenta. Purification and some properties. An enzyme hydrolyzing N-acetylaminoacyl-p-nitroanilides has been isolated from mature human placentae by a six-step procedure comprising extraction from a placenta homogenate, ammonium sulfate fractionation, treatment with isopentyl alcohol, chromatography on CM-Sephadex, protamine sulfate precipitation and gel filtration on an Ultrogel AcA 34 column. About 2500-fold enrichement was achieved from placenta homogenate. The purified enzyme preparation showed a single band on polyacrylamide disc electrophoresis. The molecular weight was estimated to be 380,000 by gel filtration. Placental extracts contain two main isoenzymes of pI 3.9 and 4.5 respectively. Activity was strongly inhibited by chloromercuribenzoate, slightly inhibited by Ca2+ and cysteine; no activation could be detected. The enzyme exhibits an exopeptidase-like activity towards acetyl-dipeptides with a certain specifity towards N-acetylalanyl-alanine; N-acetylalanine-p-nitroanilide, however, is hydrolyzed four times faster. With N-acetylalanine-p-nitroanilide as substrate the pH optimum was 8.0--8.2; Km was 2.13 mmol/l. N-Acetylleucine-p-nitroanilide and N-acetyltyrosine-p-nitroanilide were split slowly; N-acetylalanyl-alanyl-alanine-p-nitroanilide, N-butyloxycarbonyl-alanyl-alanine-p-nitroanilide, unsubstituted analogous aminoacyl-p-nitroanilides and several protein substrates were not hydrolyzed. The functions of the enzyme are still unknown."}
{"id": "PMID:477669", "title": "Binding of Semliki Forest virus and its spike glycoproteins to cells.", "content": "We have studied the binding of the Semliki Forest virus and its isolated spike glycoproteins, in the form of water-soluble octameric complexes, to various cells at 5 degrees C. The number of viruses bound per cell increased strongly with increasing free concentrations of virus up to about 0.2 nM. At higher concentrations smaller increases in binding were observed but saturation was not achieved. The number of viruses bound at a given free concentration was widely different for different cells. For some cells the binding of the virus was maximal at pH 6.8 with little decrease at lower pH, for other cells it was maximal around pH 6.0. The spike protein complexes were used at 100 times higher molar concentrations than the virus. The binding increased strongly with increasing free concentrations up to about 50 nM and saturation was obtained at higher concentrations. Up to 1.3 X 10(6) spike protein complexes could be bound per cell but great variation could be seen between different cell types. For all cells maximal binding was found below pH 6.0. Together with earlier observations, our results suggest that the virus can bind to a cell by two different modes. Around neutral pH the virus binds to specific glycoproteins and at low pH unspecifically to the lipids of the plasma membrane. The possible physiological roles of these two types of binding are discussed.", "contents": "Binding of Semliki Forest virus and its spike glycoproteins to cells. We have studied the binding of the Semliki Forest virus and its isolated spike glycoproteins, in the form of water-soluble octameric complexes, to various cells at 5 degrees C. The number of viruses bound per cell increased strongly with increasing free concentrations of virus up to about 0.2 nM. At higher concentrations smaller increases in binding were observed but saturation was not achieved. The number of viruses bound at a given free concentration was widely different for different cells. For some cells the binding of the virus was maximal at pH 6.8 with little decrease at lower pH, for other cells it was maximal around pH 6.0. The spike protein complexes were used at 100 times higher molar concentrations than the virus. The binding increased strongly with increasing free concentrations up to about 50 nM and saturation was obtained at higher concentrations. Up to 1.3 X 10(6) spike protein complexes could be bound per cell but great variation could be seen between different cell types. For all cells maximal binding was found below pH 6.0. Together with earlier observations, our results suggest that the virus can bind to a cell by two different modes. Around neutral pH the virus binds to specific glycoproteins and at low pH unspecifically to the lipids of the plasma membrane. The possible physiological roles of these two types of binding are discussed."}
{"id": "PMID:477670", "title": "Methyl acceptors for protein methylase II from human-erythrocyte membrane.", "content": "Membrane proteins from human erythrocytes were methylated with purified protein methylase II (S-adenosylmethionine:protein-carboxyl O-methyltransferase, EC.2.1.1.24). The methylated proteins were analyzed by dodecyl sulfate/polyacrylamide gel electrophoresis. Monomeric and dimeric glycophorin A (NaIO4/Schiff-2 and NaIO4/Schiff-1 positive bands) and 'band 4.5' were identified as two major classes of methyl-acceptor polypeptides for protein methylase II. In rabbit erythrocyte membrane where glycophorin A is absent, 'band 4.5' was the only major methyl-acceptor protein component. Extracted and purified glycophorin A from human erythrocytes was also found to be an excellent substrate for protein methylase II with a Km of 35.7 microM. The role of erythrocyte membrane protein methylation is discussed with regard to membrane function.", "contents": "Methyl acceptors for protein methylase II from human-erythrocyte membrane. Membrane proteins from human erythrocytes were methylated with purified protein methylase II (S-adenosylmethionine:protein-carboxyl O-methyltransferase, EC.2.1.1.24). The methylated proteins were analyzed by dodecyl sulfate/polyacrylamide gel electrophoresis. Monomeric and dimeric glycophorin A (NaIO4/Schiff-2 and NaIO4/Schiff-1 positive bands) and 'band 4.5' were identified as two major classes of methyl-acceptor polypeptides for protein methylase II. In rabbit erythrocyte membrane where glycophorin A is absent, 'band 4.5' was the only major methyl-acceptor protein component. Extracted and purified glycophorin A from human erythrocytes was also found to be an excellent substrate for protein methylase II with a Km of 35.7 microM. The role of erythrocyte membrane protein methylation is discussed with regard to membrane function."}
{"id": "PMID:477671", "title": "Permissive effect of dexamethasone on glucagon induction of urea-cycle enzymes in perifused primary monolayer cultures of rat hepatocytes.", "content": "Parenchymal cells from adult rat liver, cultured in perifused monolayers, increased the levels of urea-cycle enzymes between 15% and 60% in response to glucagon within 24 h. This stimulation was drastically enhanced by the simultaneous presence of dexamethasone, especially in the case of argininosuccinate synthetase and argininosuccinate lyase, which increased nearly threefold. Dexamethasone itself produced only negligible stimulation, but exerted a similar effect on the stimulatory action of glucagon, if it was exclusively present during 6 h prior to the glucagon treatment, suggesting a permissive action of this hormone. The effect of glucagon, particularly in the presence of dexamethasone, was mimicked by dibutyryl adenosine 3':5'-monophosphate, whereas epinephrine was ineffective. All stimulations induced by hormones or dibutyryl adenosine 3':5'-monophosphate were abolished by cycloheximide, suggesting the involvement of protein synthesis in the induction process. Using the usual culture technique with a discontinuous supply of medium no significant effect of glucagon and dexamethasone could be measured. This striking difference between both culture systems indicates that perifusion is the more adequate in vitro system for studies of the regulation of enzyme levels. Possible reasons for the failure of hormonal stimulation of urea-cycle enzymes in normal monolayer culture are discussed.", "contents": "Permissive effect of dexamethasone on glucagon induction of urea-cycle enzymes in perifused primary monolayer cultures of rat hepatocytes. Parenchymal cells from adult rat liver, cultured in perifused monolayers, increased the levels of urea-cycle enzymes between 15% and 60% in response to glucagon within 24 h. This stimulation was drastically enhanced by the simultaneous presence of dexamethasone, especially in the case of argininosuccinate synthetase and argininosuccinate lyase, which increased nearly threefold. Dexamethasone itself produced only negligible stimulation, but exerted a similar effect on the stimulatory action of glucagon, if it was exclusively present during 6 h prior to the glucagon treatment, suggesting a permissive action of this hormone. The effect of glucagon, particularly in the presence of dexamethasone, was mimicked by dibutyryl adenosine 3':5'-monophosphate, whereas epinephrine was ineffective. All stimulations induced by hormones or dibutyryl adenosine 3':5'-monophosphate were abolished by cycloheximide, suggesting the involvement of protein synthesis in the induction process. Using the usual culture technique with a discontinuous supply of medium no significant effect of glucagon and dexamethasone could be measured. This striking difference between both culture systems indicates that perifusion is the more adequate in vitro system for studies of the regulation of enzyme levels. Possible reasons for the failure of hormonal stimulation of urea-cycle enzymes in normal monolayer culture are discussed."}
{"id": "PMID:477672", "title": "Ribosome biosynthesis is not necessary for initiation of DNA replication.", "content": "Synthesis of mature 28-S ribosomal RNA and 60-S ribosomal subunits is inhibited in baby hamster kidney (BHK) cell line ts 422E at non-permissive temperature (39 degrees C). This leads to a 66% decrease of total ribosomes per cell, a marked imbalance between the large and small ribosomal subunits in the cytoplasm and a decrease of cells per dish after prolonged culture at 30 degrees C. However, inhibition of ribosome synthesis does not affect progression of cells through the G1 period of the cell division cycle, the length of the pre-replicative period, and the rate of entry of cells into S phase. In contrast to culture at non-permissive temperature, culture of BHK ts 422E cells in the presence of 0.04 micrograms/ml actinomycin D at 33 degrees C inhibits markedly the entry into S period. It is concluded that low doses of actinomycin D exert their inhibitory effect on cell growth by preventing maturation and transport of mRNA rather than by interfering with ribosome synthesis. Microfluorometric analysis revealed only slight differences in the distribution of BHK ts 422E cells in G1, S and G2 phases of the cycle either when cultured at 33 degrees C or at 39 degrees C. When too few ribosomes per cell are produced in BHK ts 422E cells at 39 degrees C, cells do not seem to be arrested reversibly at a specific point of the cell cycle but rather to die at random.", "contents": "Ribosome biosynthesis is not necessary for initiation of DNA replication. Synthesis of mature 28-S ribosomal RNA and 60-S ribosomal subunits is inhibited in baby hamster kidney (BHK) cell line ts 422E at non-permissive temperature (39 degrees C). This leads to a 66% decrease of total ribosomes per cell, a marked imbalance between the large and small ribosomal subunits in the cytoplasm and a decrease of cells per dish after prolonged culture at 30 degrees C. However, inhibition of ribosome synthesis does not affect progression of cells through the G1 period of the cell division cycle, the length of the pre-replicative period, and the rate of entry of cells into S phase. In contrast to culture at non-permissive temperature, culture of BHK ts 422E cells in the presence of 0.04 micrograms/ml actinomycin D at 33 degrees C inhibits markedly the entry into S period. It is concluded that low doses of actinomycin D exert their inhibitory effect on cell growth by preventing maturation and transport of mRNA rather than by interfering with ribosome synthesis. Microfluorometric analysis revealed only slight differences in the distribution of BHK ts 422E cells in G1, S and G2 phases of the cycle either when cultured at 33 degrees C or at 39 degrees C. When too few ribosomes per cell are produced in BHK ts 422E cells at 39 degrees C, cells do not seem to be arrested reversibly at a specific point of the cell cycle but rather to die at random."}
{"id": "PMID:477673", "title": "Purification and properties of a histone acetyltransferase from Artemia salina, highly efficient with H1 histone.", "content": "An histone acetyltransferase has been purified from nuclei of 40-h-old Artemia salina larvae. The enzyme is very unstable at 0 degrees C, requires free -SH groups for activity and is rapidly inactivated at 40 degrees C. The optimal pH for activity is 8.5 and the activity is half inhibited by millimolar concentrations of Mn2+, Ca2+ or Mg2+ or decimolar concentrations of Na+ and K+. The molecular weight of the enzyme, determined by gel filtration chromatography, changed with the ionic strength of the medium (280,000 in 10 mM Tris . HCl, 170,000 in 0.2 M KCl). The very-lysine-rich histone H1 is a better substrate acceptor than the arginine-rich histones H3 or H4. Under proper conditions, the enzyme can modify all the internal lysyl residues in histones H1 and H4. The acetylation of H1 is inhibited when all the other histone fractions are present in the assay mixture.", "contents": "Purification and properties of a histone acetyltransferase from Artemia salina, highly efficient with H1 histone. An histone acetyltransferase has been purified from nuclei of 40-h-old Artemia salina larvae. The enzyme is very unstable at 0 degrees C, requires free -SH groups for activity and is rapidly inactivated at 40 degrees C. The optimal pH for activity is 8.5 and the activity is half inhibited by millimolar concentrations of Mn2+, Ca2+ or Mg2+ or decimolar concentrations of Na+ and K+. The molecular weight of the enzyme, determined by gel filtration chromatography, changed with the ionic strength of the medium (280,000 in 10 mM Tris . HCl, 170,000 in 0.2 M KCl). The very-lysine-rich histone H1 is a better substrate acceptor than the arginine-rich histones H3 or H4. Under proper conditions, the enzyme can modify all the internal lysyl residues in histones H1 and H4. The acetylation of H1 is inhibited when all the other histone fractions are present in the assay mixture."}
{"id": "PMID:477674", "title": "1H-Nuclear magnetic-resonance studies on glycophorin and its carbohydrate-containing tryptic peptides.", "content": "The proton nuclear magnetic resonance (1H-NMR) spectra of glycophorin and its tryptic sialoglycopeptides were investigated. From the intensities of the assigned resonances it was concluded that all of the residues in the sialoglycopeptides are sufficiently mobile in conformation to give sharp resonances, while in glycophorin this is true for only approximately 80% of the peptide backbone. The resonances of the central sequence of some 20 of the hydrophobic residues are strongly broadened. This region is probably that of alpha-helical structure which is known to aggregate. The linewidths and intensities of the resonances are not, or only slightly, affected by changing the ionic strength, temperature or by carboxymethylation of the Met-81 residue in glycophorin. Glycophorin was found to bind about 100 mol sodium dodecylsulphate/mol protein as derived from studies on linebroadening of the latter's C-3 to C-11 methylene resonances. The bound dodecyl-sulphate probably increases the mobilities of the hydrophobic residues in the protein as these resonance intensities are increased by the binding. The carbohydrate chains in glycophorin were conformationally mobile; no evidence was found for tight carbohydrate-protein interactions. The relevance of flexible carbohydrate chains in membrane glycoproteins is discussed in relation to cell surface chemistry.", "contents": "1H-Nuclear magnetic-resonance studies on glycophorin and its carbohydrate-containing tryptic peptides. The proton nuclear magnetic resonance (1H-NMR) spectra of glycophorin and its tryptic sialoglycopeptides were investigated. From the intensities of the assigned resonances it was concluded that all of the residues in the sialoglycopeptides are sufficiently mobile in conformation to give sharp resonances, while in glycophorin this is true for only approximately 80% of the peptide backbone. The resonances of the central sequence of some 20 of the hydrophobic residues are strongly broadened. This region is probably that of alpha-helical structure which is known to aggregate. The linewidths and intensities of the resonances are not, or only slightly, affected by changing the ionic strength, temperature or by carboxymethylation of the Met-81 residue in glycophorin. Glycophorin was found to bind about 100 mol sodium dodecylsulphate/mol protein as derived from studies on linebroadening of the latter's C-3 to C-11 methylene resonances. The bound dodecyl-sulphate probably increases the mobilities of the hydrophobic residues in the protein as these resonance intensities are increased by the binding. The carbohydrate chains in glycophorin were conformationally mobile; no evidence was found for tight carbohydrate-protein interactions. The relevance of flexible carbohydrate chains in membrane glycoproteins is discussed in relation to cell surface chemistry."}
{"id": "PMID:477675", "title": "Purification, some properties and the complete primary structures of two protease inhibitors (DE-3 and DE-4) from Macrotyloma axillare seed.", "content": "The Macrotyloma axillare plant, belonging to the Leguminosae family, is a perennial climbing or trailing herb 0.2--3.5 m long. The plant is indigenous to South Africa and it occurs in the warm dry northern parts of the Transvaal. It has been introduced into Australia, where the seed are used as animal food. Two protease inhibitors, DE-3 and DE-4, were purified from Macrotyloma axillare seed by gel filtration on Sephadex G-50 followed by ion-exchange chromatography on DEAE-cellulose. They each comprise 76 amino acid residues including 14 half-cystine residues. The complete primary structures of the two protease innibitors have been elucidated and their sequences are 67% identical. The inhibitor specificities, the sequences, the invariant amino acid residues and the reactive inhibitor sites of protease inhibitors DE-3 and DE-4 resemble the corresponding properties of the Bowman-Birk double-headed protease inhibitor group. The cysteine residues are in similar locations to those in protease inhibitors of known structure so they are presumed to link similarly.", "contents": "Purification, some properties and the complete primary structures of two protease inhibitors (DE-3 and DE-4) from Macrotyloma axillare seed. The Macrotyloma axillare plant, belonging to the Leguminosae family, is a perennial climbing or trailing herb 0.2--3.5 m long. The plant is indigenous to South Africa and it occurs in the warm dry northern parts of the Transvaal. It has been introduced into Australia, where the seed are used as animal food. Two protease inhibitors, DE-3 and DE-4, were purified from Macrotyloma axillare seed by gel filtration on Sephadex G-50 followed by ion-exchange chromatography on DEAE-cellulose. They each comprise 76 amino acid residues including 14 half-cystine residues. The complete primary structures of the two protease innibitors have been elucidated and their sequences are 67% identical. The inhibitor specificities, the sequences, the invariant amino acid residues and the reactive inhibitor sites of protease inhibitors DE-3 and DE-4 resemble the corresponding properties of the Bowman-Birk double-headed protease inhibitor group. The cysteine residues are in similar locations to those in protease inhibitors of known structure so they are presumed to link similarly."}
{"id": "PMID:477676", "title": "A double-blind pediatric evaluation of levamisole in the prevention of recurrent upper respiratory tract infections.", "content": "Levamisole was tested double-blind in 106 children with recurrent upper respiratory tract infections. They received either levamisole (n = 53) or placebo (n = 53) 0.5 ml/kg bodyweight b.i.d. for two consecutive days each week for six months. A control examination was performed every two months. Both groups were compared by means of the Fisher-test and the Mann-Whitney U-test (two-tailed probability each). Improvement was observed more frequently in the levamisole group with regard to the number of episodes of infection, and the total duration and severity of the infections. No side-effects, except for some stomach complaints in one levamisole patient, were reported.", "contents": "A double-blind pediatric evaluation of levamisole in the prevention of recurrent upper respiratory tract infections. Levamisole was tested double-blind in 106 children with recurrent upper respiratory tract infections. They received either levamisole (n = 53) or placebo (n = 53) 0.5 ml/kg bodyweight b.i.d. for two consecutive days each week for six months. A control examination was performed every two months. Both groups were compared by means of the Fisher-test and the Mann-Whitney U-test (two-tailed probability each). Improvement was observed more frequently in the levamisole group with regard to the number of episodes of infection, and the total duration and severity of the infections. No side-effects, except for some stomach complaints in one levamisole patient, were reported."}
{"id": "PMID:477678", "title": "Coagulation studies on umbilical arterial and venous blood from normal newborn babies.", "content": "Coagulation studies using conventional methods and chromogenic substrates were performed on umbilical arterial and venous blood from 33 newborns after delivery. In the arterial samples, thrombin time (TT) was significantly prolonged and the activities of factors I, II, V and VII, as well as the inhibitors heparin, antithrombin III and antiplasmin, were significantly decreased. This could probably be explained by a mild form of disseminated intravascular coagulation (DIC) occurring in the baby during delivery.", "contents": "Coagulation studies on umbilical arterial and venous blood from normal newborn babies. Coagulation studies using conventional methods and chromogenic substrates were performed on umbilical arterial and venous blood from 33 newborns after delivery. In the arterial samples, thrombin time (TT) was significantly prolonged and the activities of factors I, II, V and VII, as well as the inhibitors heparin, antithrombin III and antiplasmin, were significantly decreased. This could probably be explained by a mild form of disseminated intravascular coagulation (DIC) occurring in the baby during delivery."}
{"id": "PMID:477677", "title": "Pattern of growth hormone response to insulin, arginine and haemodialysis in uraemic children.", "content": "Plasma growth hormone (GH) concentrations after insulin and arginine stimulation were estimated in 11 dialyzed and 6 non-dialyzed children with chronic renal failure. Twenty healthy children served as controls. Plasma GH peak concentration and estimation of the total area under the plasma GH concentration-time curve by the trapezoidal rule were used to evaluate results. Elevated basal GH levels and an exaggerated response to the stimuli were seen in several of the patients. The causes of the abnormal GH secretion and the role of high GH levels in carbohydrate intolerance are discussed. No consistent pattern was seen in GH secretion during haemodialysis without glucose in the dialysate. In children undergoing haemodialysis with a fluid containing glucose, plasma GH fell considerably.", "contents": "Pattern of growth hormone response to insulin, arginine and haemodialysis in uraemic children. Plasma growth hormone (GH) concentrations after insulin and arginine stimulation were estimated in 11 dialyzed and 6 non-dialyzed children with chronic renal failure. Twenty healthy children served as controls. Plasma GH peak concentration and estimation of the total area under the plasma GH concentration-time curve by the trapezoidal rule were used to evaluate results. Elevated basal GH levels and an exaggerated response to the stimuli were seen in several of the patients. The causes of the abnormal GH secretion and the role of high GH levels in carbohydrate intolerance are discussed. No consistent pattern was seen in GH secretion during haemodialysis without glucose in the dialysate. In children undergoing haemodialysis with a fluid containing glucose, plasma GH fell considerably."}
{"id": "PMID:477679", "title": "Two sisters with isovaleric acidaemia, multiple attacks of ketoacidosis and normal development.", "content": "Two sisters with isovaleric acidaemia are described. Both had multiple attacks of acetonaemic vomiting, sometimes leading to subcoma. Despite this they showed a completely normal mental development. Biochemical studies, clinical follow-up and attempts at treatment are presented.", "contents": "Two sisters with isovaleric acidaemia, multiple attacks of ketoacidosis and normal development. Two sisters with isovaleric acidaemia are described. Both had multiple attacks of acetonaemic vomiting, sometimes leading to subcoma. Despite this they showed a completely normal mental development. Biochemical studies, clinical follow-up and attempts at treatment are presented."}
{"id": "PMID:477680", "title": "Diaphragmatic hernia in a female newborn with focal dermal hypoplasia and marked asymmetric malformations (Goltz-Gorlin syndrome).", "content": "We report a female newborn with focal dermal hypoplasia (Goltz-Gorlin Syndrome) and marked asymmetric malformations on the right side of the body. Diaphragmatic hernia on the same side, which has not been reported in this syndrome, led to perinatal complications.", "contents": "Diaphragmatic hernia in a female newborn with focal dermal hypoplasia and marked asymmetric malformations (Goltz-Gorlin syndrome). We report a female newborn with focal dermal hypoplasia (Goltz-Gorlin Syndrome) and marked asymmetric malformations on the right side of the body. Diaphragmatic hernia on the same side, which has not been reported in this syndrome, led to perinatal complications."}
{"id": "PMID:477681", "title": "Biochemical assessment of thiamine nutrition in childhood.", "content": "The activity of red cell transketolase and TPP-effect were determined in 1028 infants and children, aged 1 month to 14 years, using a micromethod. All the children were healthy and without evidence of malnutrition. Transketolase activity decreased in the second year of life, followed by a constant increase up to the 6th year. Activity did not change in the next four years, then a steady decrease occurred. TPP-effect was not as stable as transketolase activity, and decreased significantly from years 3 to 5 and increased significantly from years 5 to 7. Beginning with year 10, TPP-effect decreased gradually. 6.6% of all children investigated had marginally deficient TPP-effects and 1.3% were biochemically deficient without clinical signs of avitaminosis. A group of 37 adults had transketolase activities and TPP-effect comparable to those of the 13 year-olds. Effect appeared to be marginal in 5.4% and deficient in 2.7%.", "contents": "Biochemical assessment of thiamine nutrition in childhood. The activity of red cell transketolase and TPP-effect were determined in 1028 infants and children, aged 1 month to 14 years, using a micromethod. All the children were healthy and without evidence of malnutrition. Transketolase activity decreased in the second year of life, followed by a constant increase up to the 6th year. Activity did not change in the next four years, then a steady decrease occurred. TPP-effect was not as stable as transketolase activity, and decreased significantly from years 3 to 5 and increased significantly from years 5 to 7. Beginning with year 10, TPP-effect decreased gradually. 6.6% of all children investigated had marginally deficient TPP-effects and 1.3% were biochemically deficient without clinical signs of avitaminosis. A group of 37 adults had transketolase activities and TPP-effect comparable to those of the 13 year-olds. Effect appeared to be marginal in 5.4% and deficient in 2.7%."}
{"id": "PMID:477682", "title": "Hypercalciuria in idiopathic Fanconi syndrome.", "content": "A 9 year old girl with idiopathic Fanconi syndrome and hypercalciuria is described. In order to determine whether the increased calcium excretion was directly or indirectly due to the disturbed phosphate metabolism, the behavior of the calcium excretion during therapy, the serum levels of 1,25-dihydroxyvitamin D and parathyroid hormone, and the effect of parathyroid hormone on the renal tubules were investigated. Normal serum 1,25-dihydroxyvitamin D and parathyroid hormone levels, lack of a correlation between the serum phosphate concentration and the degree of hypercalciuria, as well as unsuccessful therapy of the hypercalciuria with oral phosphate indicate that the increased calcium excretion cannot be explained by impaired renal phosphate reabsorption. The hypercalciuria in the patient was therefore regarded as being due to a primary decrease of tubular calcium reabsorption.", "contents": "Hypercalciuria in idiopathic Fanconi syndrome. A 9 year old girl with idiopathic Fanconi syndrome and hypercalciuria is described. In order to determine whether the increased calcium excretion was directly or indirectly due to the disturbed phosphate metabolism, the behavior of the calcium excretion during therapy, the serum levels of 1,25-dihydroxyvitamin D and parathyroid hormone, and the effect of parathyroid hormone on the renal tubules were investigated. Normal serum 1,25-dihydroxyvitamin D and parathyroid hormone levels, lack of a correlation between the serum phosphate concentration and the degree of hypercalciuria, as well as unsuccessful therapy of the hypercalciuria with oral phosphate indicate that the increased calcium excretion cannot be explained by impaired renal phosphate reabsorption. The hypercalciuria in the patient was therefore regarded as being due to a primary decrease of tubular calcium reabsorption."}
{"id": "PMID:477683", "title": "Association between HLA-A1,B8 in children with extrinsic asthma and IgA deficiency.", "content": "The tissue types, immunoglobulin levels, and the presence of circulating autoantibodies were investigated in 57 children. Fifteen of these children suffered from bronchial asthma and, in addition, had no or very little IgA in their serum and saliva (Group 1 patients). Another fifteen children with asthma but normal immunoglobulin levels in serum and saliva (Group 2 patients), seven patients with selective IgA deficiency but without allergic diseases (Group 3 patients), and twenty healthy children served as controls. Sixty per cent of the Group 1 patients had the phenotype HLA-A1,B8, whereas this tissue type was found only in 27, 14 and 15 per cent, respectively, of the Group 2 and Group 3 patients and the healthy children. Furthermore, high IgM- and IgE levels were observed in most Group 1 patients, and in five of these patients (33 per cent) autoantibodies were present in the serum. In addition, eczema and glomerulonephritis occurred rather frequently in this group of patients. Conversely, normal immunoglobulin levels and absence of circulating autoantibodies were found in the remaining three groups of children. The results emphasize the heterogeneity of the IgA deficiency syndrome, and the question is raised as to whether the tissue type HLA-A1,B8 observed in most Group 1 patients reflects the abnormal immune reactivity of these patients.", "contents": "Association between HLA-A1,B8 in children with extrinsic asthma and IgA deficiency. The tissue types, immunoglobulin levels, and the presence of circulating autoantibodies were investigated in 57 children. Fifteen of these children suffered from bronchial asthma and, in addition, had no or very little IgA in their serum and saliva (Group 1 patients). Another fifteen children with asthma but normal immunoglobulin levels in serum and saliva (Group 2 patients), seven patients with selective IgA deficiency but without allergic diseases (Group 3 patients), and twenty healthy children served as controls. Sixty per cent of the Group 1 patients had the phenotype HLA-A1,B8, whereas this tissue type was found only in 27, 14 and 15 per cent, respectively, of the Group 2 and Group 3 patients and the healthy children. Furthermore, high IgM- and IgE levels were observed in most Group 1 patients, and in five of these patients (33 per cent) autoantibodies were present in the serum. In addition, eczema and glomerulonephritis occurred rather frequently in this group of patients. Conversely, normal immunoglobulin levels and absence of circulating autoantibodies were found in the remaining three groups of children. The results emphasize the heterogeneity of the IgA deficiency syndrome, and the question is raised as to whether the tissue type HLA-A1,B8 observed in most Group 1 patients reflects the abnormal immune reactivity of these patients."}
{"id": "PMID:477684", "title": "Trisomy 9 mosaicism with punctate mineralization in developing cartilages.", "content": "A case of mosaic trisomy 9 (46,XX/47,XX,+9) is described. The main clinical findings included intrauterine growth retardation, failure to thrive, hydrocephalus, deeply set eyes, prominent antihelix, highly arched palate, subluxation of the interphalangeal joints of some fingers, hip dislocation, excessive sweating, and punctate mineralization in developing cartilages.", "contents": "Trisomy 9 mosaicism with punctate mineralization in developing cartilages. A case of mosaic trisomy 9 (46,XX/47,XX,+9) is described. The main clinical findings included intrauterine growth retardation, failure to thrive, hydrocephalus, deeply set eyes, prominent antihelix, highly arched palate, subluxation of the interphalangeal joints of some fingers, hip dislocation, excessive sweating, and punctate mineralization in developing cartilages."}
{"id": "PMID:477685", "title": "A rapid unfavorable outcome of Wegener's granulomatosis in early childhood.", "content": "Wegener's Granulomatosis was suspected in a 27-month-old female with a nodular, necrotizing lesion of the nose, diffuse subcutaneous nodules, and erythematous desquamation of the entire body. From 20 months of age on she had a purulent nasal discharge, recurrent infections of the upper and lower respiratory tract, a Coombs positive anemia, and enlargement of the spleen and liver. Treatment with azathioprine and corticosteroids produced transient improvement but three months later a dramatic relapse occurred. Cyclophosphamide was substituted for azathioprine but 10 days later the patient died and the autopsy confirmed the diagnosis of Wegener's Granulomatosis. The early age of onset of the disease may explain the unfavorable outcome, despite treatment with cytotoxic agents.", "contents": "A rapid unfavorable outcome of Wegener's granulomatosis in early childhood. Wegener's Granulomatosis was suspected in a 27-month-old female with a nodular, necrotizing lesion of the nose, diffuse subcutaneous nodules, and erythematous desquamation of the entire body. From 20 months of age on she had a purulent nasal discharge, recurrent infections of the upper and lower respiratory tract, a Coombs positive anemia, and enlargement of the spleen and liver. Treatment with azathioprine and corticosteroids produced transient improvement but three months later a dramatic relapse occurred. Cyclophosphamide was substituted for azathioprine but 10 days later the patient died and the autopsy confirmed the diagnosis of Wegener's Granulomatosis. The early age of onset of the disease may explain the unfavorable outcome, despite treatment with cytotoxic agents."}
{"id": "PMID:477686", "title": "Prognosis of prophylactic anticoagulant treatment in ischaemic stroke.", "content": "Two groups of patients who had a stroke, prolonged reversible ischaemic neurological deficit or transient ischaemic attacks were compared; one group was treated with anticoagulants (phenprocoumon) and a matched control group was treated with other substances. Results showed a similar survival rate in both groups, but significantly fewer recurrences were seen in the surviving patients treated with anticoagulants.", "contents": "Prognosis of prophylactic anticoagulant treatment in ischaemic stroke. Two groups of patients who had a stroke, prolonged reversible ischaemic neurological deficit or transient ischaemic attacks were compared; one group was treated with anticoagulants (phenprocoumon) and a matched control group was treated with other substances. Results showed a similar survival rate in both groups, but significantly fewer recurrences were seen in the surviving patients treated with anticoagulants."}
{"id": "PMID:477687", "title": "Zoster meningitis and radiculomeningitis after tick bite. Cytological findings in cerebrospinal fluid.", "content": "Cytological changes in 9 samples of cerebrospinal fluid (CSF) from 5 patients with zoster meningitis and 12 samples from 5 patients with radiculomeningitis after tick bite were examined. 5 days after the onset of the skin eruption, the CSF cells in zoster meningitis consisted of many mononuclear blast forms--large lymphocytes and 'immature' plasma cells. 11-15 days after the onset of the skin rash, they were replaced by small lymphocytes, some 'mature' plasma cells and monohistiocytes. In radiculomeningitis after tick bite, however, the CSF cells examined 10-21 days after the onset of radicular pain consisted of many large lymphocytes, immature plasma cells and a few neutrophils. From these findings, it might be suggested that the acute meningeal reaction of zoster meningitis subsides within about 1 week after the onset of the skin rash, but that of radiculomeningitis after tick bite continues at least for 2-3 weeks after the onset of radicular pain.", "contents": "Zoster meningitis and radiculomeningitis after tick bite. Cytological findings in cerebrospinal fluid. Cytological changes in 9 samples of cerebrospinal fluid (CSF) from 5 patients with zoster meningitis and 12 samples from 5 patients with radiculomeningitis after tick bite were examined. 5 days after the onset of the skin eruption, the CSF cells in zoster meningitis consisted of many mononuclear blast forms--large lymphocytes and 'immature' plasma cells. 11-15 days after the onset of the skin rash, they were replaced by small lymphocytes, some 'mature' plasma cells and monohistiocytes. In radiculomeningitis after tick bite, however, the CSF cells examined 10-21 days after the onset of radicular pain consisted of many large lymphocytes, immature plasma cells and a few neutrophils. From these findings, it might be suggested that the acute meningeal reaction of zoster meningitis subsides within about 1 week after the onset of the skin rash, but that of radiculomeningitis after tick bite continues at least for 2-3 weeks after the onset of radicular pain."}
{"id": "PMID:477688", "title": "Reciprocal reflex activity in ocular muscles: implications in spontaneous blinking and Bell's phenomenon.", "content": "Levator palpebrae (m. lev. palp.) and rectus superior (m. rect. sup.) are two synergistic muscles in vertical gaze movements. However, they show an antagonistic behavior during electrically induced blink and glabellar reflexes. This can be considered as an easy and useful method for their differentiation in the electrophysiological study of certain oculopalpebral motor disorders. Blinking consists of a series of reflex activities, the most outstanding ones being an early m. lev. palp. basal activity inhibition, followed by the activation of the orbicularis oculi muscle and a brief increase in m. rect. sup. activity (reflex Bell phenomenon). This coordinated reflex organization could be perfectly related to that known in other human nociceptive reflexes.", "contents": "Reciprocal reflex activity in ocular muscles: implications in spontaneous blinking and Bell's phenomenon. Levator palpebrae (m. lev. palp.) and rectus superior (m. rect. sup.) are two synergistic muscles in vertical gaze movements. However, they show an antagonistic behavior during electrically induced blink and glabellar reflexes. This can be considered as an easy and useful method for their differentiation in the electrophysiological study of certain oculopalpebral motor disorders. Blinking consists of a series of reflex activities, the most outstanding ones being an early m. lev. palp. basal activity inhibition, followed by the activation of the orbicularis oculi muscle and a brief increase in m. rect. sup. activity (reflex Bell phenomenon). This coordinated reflex organization could be perfectly related to that known in other human nociceptive reflexes."}
{"id": "PMID:477689", "title": "A genetic study of febrile convulsions.", "content": "307 probands with febrile convulsions classed as the simple type (131 children) and the complicated type (176 children) were genetically analyzed. There was a tendency toward familial aggregation of febrile convulsions, and genetic involvement was suggested. The multifactorial mode of inheritance best agreed with the observations. (1) The ratio of incidence of febrile convulsions in siblings of probands in the present study (19.9%) to the incidence in the general population (2.9%), i.e., 6.85, was rather close to the expected ratio of 5.87 from the multifactorial inheritance system. (2) The incidence in siblings tended to be higher when either or both of their parents had a history of febrile convulsions. In 2-child families where the first child was affected, the incidence in the second child tended to increase in parallel with the increasing incidence in parents. (3) The incidence in siblings was higher if probands were males. (4) The heritability, when estimated by Falconer's procedure, was as high as 76%, showing that febrile convulsions are strongly genetically predisposed. These findings were more distinctly observed in the simple type than in the complicated type.", "contents": "A genetic study of febrile convulsions. 307 probands with febrile convulsions classed as the simple type (131 children) and the complicated type (176 children) were genetically analyzed. There was a tendency toward familial aggregation of febrile convulsions, and genetic involvement was suggested. The multifactorial mode of inheritance best agreed with the observations. (1) The ratio of incidence of febrile convulsions in siblings of probands in the present study (19.9%) to the incidence in the general population (2.9%), i.e., 6.85, was rather close to the expected ratio of 5.87 from the multifactorial inheritance system. (2) The incidence in siblings tended to be higher when either or both of their parents had a history of febrile convulsions. In 2-child families where the first child was affected, the incidence in the second child tended to increase in parallel with the increasing incidence in parents. (3) The incidence in siblings was higher if probands were males. (4) The heritability, when estimated by Falconer's procedure, was as high as 76%, showing that febrile convulsions are strongly genetically predisposed. These findings were more distinctly observed in the simple type than in the complicated type."}
{"id": "PMID:477690", "title": "Normal functional capacity of blood lymphocytes in multiple sclerosis measured by stimulation with mitogens.", "content": "The effect of phytohemagglutinin (PHA) and pokeweed mitogen (PWM) on blood lymphocytes was studied in multiple sclerosis, patients with acute CNS infections, and healthy controls. No significant differences in lymphocyte proliferation were registered between the group of MS patients and the two other groups, irrespective of mitogen used. The ratio between the PWM and PHA responses did not differ significantly from the ratios in the two other groups. The lymphocyte proliferation with PWM was significantly lower in the group with acute CNS infections compared with controls. The results indicate that alterations in cell-mediated immunity, if present in MS, are not confined to lymphocytes outside the CNS.", "contents": "Normal functional capacity of blood lymphocytes in multiple sclerosis measured by stimulation with mitogens. The effect of phytohemagglutinin (PHA) and pokeweed mitogen (PWM) on blood lymphocytes was studied in multiple sclerosis, patients with acute CNS infections, and healthy controls. No significant differences in lymphocyte proliferation were registered between the group of MS patients and the two other groups, irrespective of mitogen used. The ratio between the PWM and PHA responses did not differ significantly from the ratios in the two other groups. The lymphocyte proliferation with PWM was significantly lower in the group with acute CNS infections compared with controls. The results indicate that alterations in cell-mediated immunity, if present in MS, are not confined to lymphocytes outside the CNS."}
{"id": "PMID:477691", "title": "A study on Sturge-Weber syndrome. Report of a case associated with infantile spasms and electroencephalographic evolution in five cases.", "content": "A case of classical Sturge-Weber syndrome associated with infantile spasms (female infant, aged 4 months) was presented. The association of both conditions would be very rare since no similar case was found among 214 cases of Sturge-Weber syndrome and 1,180 cases of infantile spasms gathered from the literature, except for one who was reported by Millichap et al. Evolutional changes of electroencephalographic findings were followed up for about 3 years on average in 5 personal cases of Sturge-Weber syndrome. Unilateral depression of electrical activity in the cerebral hemisphere ipsilateral to the facial nevi was the constant finding. Focal spike discharges were noticed only in the contralateral hemisphere in 3 cases, only in the ipsilateral in 1, and in the bilateral in 1.", "contents": "A study on Sturge-Weber syndrome. Report of a case associated with infantile spasms and electroencephalographic evolution in five cases. A case of classical Sturge-Weber syndrome associated with infantile spasms (female infant, aged 4 months) was presented. The association of both conditions would be very rare since no similar case was found among 214 cases of Sturge-Weber syndrome and 1,180 cases of infantile spasms gathered from the literature, except for one who was reported by Millichap et al. Evolutional changes of electroencephalographic findings were followed up for about 3 years on average in 5 personal cases of Sturge-Weber syndrome. Unilateral depression of electrical activity in the cerebral hemisphere ipsilateral to the facial nevi was the constant finding. Focal spike discharges were noticed only in the contralateral hemisphere in 3 cases, only in the ipsilateral in 1, and in the bilateral in 1."}
{"id": "PMID:477692", "title": "Oral zinc sulphate as long-term treatment in Wilson's disease (hepatolenticular degeneration).", "content": "Clinical amelioration, clearance of Kayser-Fleischer rings and rising of ceruloplasmin concentration are described in a patient with the classical findings of Wilson's disease. These changes occurred during a 14-year period in which he used oral zinc sulphate (three times daily 200 mg) as the only medication to influence copper metabolism. Before starting this long-term zinc sulphate therapy he had used D-penicillamine (three times daily 300 mg) for only 6 weeks. The antagonistic action of zinc sulphate on copper resorption with amelioration of the clinical condition has been described before in this patient in 1961 by Schouwink. The patient had used at that time oral zinc sulphate for approximately 1.5 years. No changes in Kayser-Fleischer rings and ceruloplasmin levels were mentioned. Our findings suggest that oral zinc sulphate may not only prevent storage of copper in the tissues but may also contribute to the mobilization and excretion of deposits of copper.", "contents": "Oral zinc sulphate as long-term treatment in Wilson's disease (hepatolenticular degeneration). Clinical amelioration, clearance of Kayser-Fleischer rings and rising of ceruloplasmin concentration are described in a patient with the classical findings of Wilson's disease. These changes occurred during a 14-year period in which he used oral zinc sulphate (three times daily 200 mg) as the only medication to influence copper metabolism. Before starting this long-term zinc sulphate therapy he had used D-penicillamine (three times daily 300 mg) for only 6 weeks. The antagonistic action of zinc sulphate on copper resorption with amelioration of the clinical condition has been described before in this patient in 1961 by Schouwink. The patient had used at that time oral zinc sulphate for approximately 1.5 years. No changes in Kayser-Fleischer rings and ceruloplasmin levels were mentioned. Our findings suggest that oral zinc sulphate may not only prevent storage of copper in the tissues but may also contribute to the mobilization and excretion of deposits of copper."}
{"id": "PMID:477693", "title": "Facial palsy as the initial symptom of a lymphoreticular malignancy. Case report.", "content": "In this case report an adult patient is described who initially manifested a peripheral facial palsy mimicking 'Bell's palsy', which later on appeared to be the first manifestation of a lymphoreticular malignancy. Cranial nerve involvement appears to be very rare as a first symptom of malignant systemic disease. The importance of additional investigations including lumbar puncture and CSF cytology in cases of facial nerve palsy is stressed.", "contents": "Facial palsy as the initial symptom of a lymphoreticular malignancy. Case report. In this case report an adult patient is described who initially manifested a peripheral facial palsy mimicking 'Bell's palsy', which later on appeared to be the first manifestation of a lymphoreticular malignancy. Cranial nerve involvement appears to be very rare as a first symptom of malignant systemic disease. The importance of additional investigations including lumbar puncture and CSF cytology in cases of facial nerve palsy is stressed."}
{"id": "PMID:477694", "title": "Effect of partial gastric devascularization on mucosal blood flow and acid secretion in cats.", "content": "Partial devascularization of the stomach was performed in cats anesthetized with pentobarbital. The devascularization included all blood vessels along the greater curvature and two of the main branches of the left gastric artery. Gastric mucosal blood flow and cardiac output were determined by means of the microsphere distribution technique. The acid (H+) output from the 30th to the 60th min of pentagastrin stimulation was used as the 'acid response value'. Blood flow and acid secretion were determined before and approximately 2 h after devascularization or sham operation. A marked decrease in acid secretion and mucosal blood flow of the corpus/fundus occurred after devascularization. A high degree of correlation was found between the decrease in acid secretion and the decrease in mucosal blood flow caused by the devascularization. The regression coefficient of acid secretion on mucosal blood flow did not change significantly after devascularization.", "contents": "Effect of partial gastric devascularization on mucosal blood flow and acid secretion in cats. Partial devascularization of the stomach was performed in cats anesthetized with pentobarbital. The devascularization included all blood vessels along the greater curvature and two of the main branches of the left gastric artery. Gastric mucosal blood flow and cardiac output were determined by means of the microsphere distribution technique. The acid (H+) output from the 30th to the 60th min of pentagastrin stimulation was used as the 'acid response value'. Blood flow and acid secretion were determined before and approximately 2 h after devascularization or sham operation. A marked decrease in acid secretion and mucosal blood flow of the corpus/fundus occurred after devascularization. A high degree of correlation was found between the decrease in acid secretion and the decrease in mucosal blood flow caused by the devascularization. The regression coefficient of acid secretion on mucosal blood flow did not change significantly after devascularization."}
{"id": "PMID:477695", "title": "Experimental acute pancreatitis: action of atropine and beta-haloethylamine furoate on muscarine-induced exocrine pancreatic secretion.", "content": "Intraarterially administered muscarine induced predictable and reproducible experimental acute pancreatitis with a simultaneous increase in amylase levels in blood. Muscarine also caused a transient rise followed by a lowering of blood glucose levels. The stimulated amylase secretion was dose-response related. The guinea pigs survived 2--2 1/2 h after muscarine administration. Atropine (3 and 5 mg/kg), an antimuscarinic agent, injected intraperitoneally 2 h prior to muscarine administration, (a) inhibited muscarine-induced amylase secretion, and (b) marginally increased the survival time of guinea pigs, but could not sustain the animals for further experimentation. The high death rate of experimental animals prevented the use of this method as a model for investigation of experimental acute pancreatitis.", "contents": "Experimental acute pancreatitis: action of atropine and beta-haloethylamine furoate on muscarine-induced exocrine pancreatic secretion. Intraarterially administered muscarine induced predictable and reproducible experimental acute pancreatitis with a simultaneous increase in amylase levels in blood. Muscarine also caused a transient rise followed by a lowering of blood glucose levels. The stimulated amylase secretion was dose-response related. The guinea pigs survived 2--2 1/2 h after muscarine administration. Atropine (3 and 5 mg/kg), an antimuscarinic agent, injected intraperitoneally 2 h prior to muscarine administration, (a) inhibited muscarine-induced amylase secretion, and (b) marginally increased the survival time of guinea pigs, but could not sustain the animals for further experimentation. The high death rate of experimental animals prevented the use of this method as a model for investigation of experimental acute pancreatitis."}
{"id": "PMID:477696", "title": "Early metabolic disturbances in the liver following unilateral hepatic or common bile duct obstruction in rabbits.", "content": "The early metabolic disturbances in the liver following ligation of the left hepatic or the common duct were studied in the rabbit. In the rabbits subjected to ligation of the left hepatic bile duct, the phosphorylative activity of the liver mitochondria in the ligated lobe decreased to about 50% of controls 48 h after the ligation, while that in the nonligated lobe increased 2-fold. These rabbits continued to live. On the other hand, in the rabbits subjected to ligation of the common bile duct, the mitochondrial phosphorylative activity increased slightly at 3 h after the ligation but fell rapidly thereafter. The mitochondrial free NAD+ to NADH ratios and hepatic energy charge (ATP+1/2ADP/ATP+ADP+AMP) markedly decreased after the ligation. Most of these rabbits did not survive over 2 days. These results indicate that the metabolic disturbances in the jaundiced liver are due to an impairment of the oxidative phosphorylation. In addition, it is suggested that the maintenance of the energy balance by enhanced mitochondrial phosphorylative activity is readily impaired in the jaundiced liver.", "contents": "Early metabolic disturbances in the liver following unilateral hepatic or common bile duct obstruction in rabbits. The early metabolic disturbances in the liver following ligation of the left hepatic or the common duct were studied in the rabbit. In the rabbits subjected to ligation of the left hepatic bile duct, the phosphorylative activity of the liver mitochondria in the ligated lobe decreased to about 50% of controls 48 h after the ligation, while that in the nonligated lobe increased 2-fold. These rabbits continued to live. On the other hand, in the rabbits subjected to ligation of the common bile duct, the mitochondrial phosphorylative activity increased slightly at 3 h after the ligation but fell rapidly thereafter. The mitochondrial free NAD+ to NADH ratios and hepatic energy charge (ATP+1/2ADP/ATP+ADP+AMP) markedly decreased after the ligation. Most of these rabbits did not survive over 2 days. These results indicate that the metabolic disturbances in the jaundiced liver are due to an impairment of the oxidative phosphorylation. In addition, it is suggested that the maintenance of the energy balance by enhanced mitochondrial phosphorylative activity is readily impaired in the jaundiced liver."}
{"id": "PMID:477697", "title": "Some functions of canine liver perfused ex vivo with bloodless fluorocarbon emulsion.", "content": "6 canine livers were perfused extracorporeally with bloodless fluorocarbon emulsion for 6 h. Perfusate was oxygenated in vitro with a mixture of oxygen and carbon dioxide. Biochemical studies were performed on the samples of perfusate taken from the arterial and venous lines of the perfusion circuit, and of the bile. Hepatic tissue was sampled prior to and following 6 h of perfusion for histological and biochemical studies. Changes, if any, found in the concentrations of various components of perfusate and bile, were considered due to the metabolic activity of the liver. Moderate changes of the liver architecture and a decrease of glycogen were found in hepatic tissues studied microscopically. Under the conditions of the present preliminary experiment, canine livers remained functional after 6 h of perfusion.", "contents": "Some functions of canine liver perfused ex vivo with bloodless fluorocarbon emulsion. 6 canine livers were perfused extracorporeally with bloodless fluorocarbon emulsion for 6 h. Perfusate was oxygenated in vitro with a mixture of oxygen and carbon dioxide. Biochemical studies were performed on the samples of perfusate taken from the arterial and venous lines of the perfusion circuit, and of the bile. Hepatic tissue was sampled prior to and following 6 h of perfusion for histological and biochemical studies. Changes, if any, found in the concentrations of various components of perfusate and bile, were considered due to the metabolic activity of the liver. Moderate changes of the liver architecture and a decrease of glycogen were found in hepatic tissues studied microscopically. Under the conditions of the present preliminary experiment, canine livers remained functional after 6 h of perfusion."}
{"id": "PMID:477698", "title": "Effects of somatostatin on gastric mucosal histamine and serotonin content in restrained rats.", "content": "The effects of continuous intravenous perfusion of somatostatin on the incidence of ulcers and the histamine and serotonin gastric mucosal content in rats subjected to 12 h of restraint were studied. 51 sets of Sprague-Dawley male rats (102 animals) were divided at random into two groups. Those in group I were immobilized and treated with an intravenous infusion of isotonic saline solution. Animals in group II were also immobilized and given intravenous cyclic somatostatin dissolved in isotonic saline solution; the initial dose was 25 microgram and a continuous infusion of 2.5 microgram/kg/h was administered thereafter. A decrease in the ulcer index was observed in the rats perfused with somatostatin (p less than 0.01) as well as a decrease in the histamine (p less than 0.001) and serotonin (p less than 0.01) gastric mucosal contents. These effects of somatostatin on restraint-induced stress ulcer in rats are probably to be explained by microcirculatory changes in the gastric mucosa determined by the vasoactive amines (histamine and serotonin). They constitute an important factor in the pathogenesis of these lesions.", "contents": "Effects of somatostatin on gastric mucosal histamine and serotonin content in restrained rats. The effects of continuous intravenous perfusion of somatostatin on the incidence of ulcers and the histamine and serotonin gastric mucosal content in rats subjected to 12 h of restraint were studied. 51 sets of Sprague-Dawley male rats (102 animals) were divided at random into two groups. Those in group I were immobilized and treated with an intravenous infusion of isotonic saline solution. Animals in group II were also immobilized and given intravenous cyclic somatostatin dissolved in isotonic saline solution; the initial dose was 25 microgram and a continuous infusion of 2.5 microgram/kg/h was administered thereafter. A decrease in the ulcer index was observed in the rats perfused with somatostatin (p less than 0.01) as well as a decrease in the histamine (p less than 0.001) and serotonin (p less than 0.01) gastric mucosal contents. These effects of somatostatin on restraint-induced stress ulcer in rats are probably to be explained by microcirculatory changes in the gastric mucosa determined by the vasoactive amines (histamine and serotonin). They constitute an important factor in the pathogenesis of these lesions."}
{"id": "PMID:477699", "title": "Systolic time intervals in middle-aged men according to smoking habits and other risk factors of coronary heart disease.", "content": "In the course of a prospective study on coronary heart disease (CHD) risk factors, systolic time intervals (STI) were measured at rest in 92 men aged 52--56 yr and free of any clinical symptoms of CHD. The ratio preejection period/left ventricular ejection period (PEP/LVEP) was significantly augmented in current smokers vs nonsmokers (0.406 +/- 0.075 vs 0.384 +/- 0.068, P less than 0.05). The regression of this parameter on cigarette consumption per day, taking into account heart rate, systolic blood pressure, body mass index, serum cholesterol and triglycerides, remains significant. An acute experiment, involving 50 smokers recruited similarly, showed no significant variation of the STI after the smoking of 2 cigarettes. We concluded that the augmentation of the PEP/LVEP ratio may represent a chronic effect of tobacco smoking on cardiac function.", "contents": "Systolic time intervals in middle-aged men according to smoking habits and other risk factors of coronary heart disease. In the course of a prospective study on coronary heart disease (CHD) risk factors, systolic time intervals (STI) were measured at rest in 92 men aged 52--56 yr and free of any clinical symptoms of CHD. The ratio preejection period/left ventricular ejection period (PEP/LVEP) was significantly augmented in current smokers vs nonsmokers (0.406 +/- 0.075 vs 0.384 +/- 0.068, P less than 0.05). The regression of this parameter on cigarette consumption per day, taking into account heart rate, systolic blood pressure, body mass index, serum cholesterol and triglycerides, remains significant. An acute experiment, involving 50 smokers recruited similarly, showed no significant variation of the STI after the smoking of 2 cigarettes. We concluded that the augmentation of the PEP/LVEP ratio may represent a chronic effect of tobacco smoking on cardiac function."}
{"id": "PMID:477700", "title": "'Bradycardia-tachycardia' syndrome 8 yr after correction of Fallot's tetralogy.", "content": "A 13-yr-old boy presented with atrial flutter 8 yr after surgical correction of tetralogy of Fallot; antiarrhythmic therapy caused depression of the sinoatrial node, with syncope. Disordered sinoatrial function and intraventricular conduction were demonstrated by intracardiac electrography, and appear to have resulted from the operation. Sinoatrial disease may be responsible for supraventricular arrhythmias or syncope long after operative correction of Fallot's tetralogy and may be one of the explanations for the tendency of such patients to die suddenly.", "contents": "'Bradycardia-tachycardia' syndrome 8 yr after correction of Fallot's tetralogy. A 13-yr-old boy presented with atrial flutter 8 yr after surgical correction of tetralogy of Fallot; antiarrhythmic therapy caused depression of the sinoatrial node, with syncope. Disordered sinoatrial function and intraventricular conduction were demonstrated by intracardiac electrography, and appear to have resulted from the operation. Sinoatrial disease may be responsible for supraventricular arrhythmias or syncope long after operative correction of Fallot's tetralogy and may be one of the explanations for the tendency of such patients to die suddenly."}
{"id": "PMID:477701", "title": "Sinus node disease affecting both parents and both children.", "content": "A family is described in which both parents and both of their children developed conducting system disease before the age of 40. They all had evidence of possible sinus node dysfunction. The father and son had an unexplained polyneuritis during childhood.", "contents": "Sinus node disease affecting both parents and both children. A family is described in which both parents and both of their children developed conducting system disease before the age of 40. They all had evidence of possible sinus node dysfunction. The father and son had an unexplained polyneuritis during childhood."}
{"id": "PMID:477702", "title": "Coarctation of the aorta operated upon in infancy. Long-term follow-up.", "content": "A long-term follow-up study was performed in 14 patients, operated for coarctation of the aorta in infancy. The median age at operation was 4 mth. Mean and median age at follow-up were 16 yr, and the follow-up time varied between 7 and 22 yr (mean and median 15 yr). At follow-up recoarctation was or had been present in 6 patients; there was no relation between the development of recoarctation and the age at operation. Concomitant aortic valve anomaly had been overlooked at the initial investigation in 3 patients. The systolic and diastolic blood pressure at follow-up was significantly elevated above the mean of normals; 2 of 8 patients without recoarctation clearly had diastolic hypertension. It seems as if even operation in infancy does not prevent hypertension later in childhood or adolescence in some of the patients, and it hardly seems justified to perform elective operations for coarctation of the aorta in infancy with the appreciable risk of recoarctation, until more long-term follow-up results are known. The patients should not be lost to follow-up.", "contents": "Coarctation of the aorta operated upon in infancy. Long-term follow-up. A long-term follow-up study was performed in 14 patients, operated for coarctation of the aorta in infancy. The median age at operation was 4 mth. Mean and median age at follow-up were 16 yr, and the follow-up time varied between 7 and 22 yr (mean and median 15 yr). At follow-up recoarctation was or had been present in 6 patients; there was no relation between the development of recoarctation and the age at operation. Concomitant aortic valve anomaly had been overlooked at the initial investigation in 3 patients. The systolic and diastolic blood pressure at follow-up was significantly elevated above the mean of normals; 2 of 8 patients without recoarctation clearly had diastolic hypertension. It seems as if even operation in infancy does not prevent hypertension later in childhood or adolescence in some of the patients, and it hardly seems justified to perform elective operations for coarctation of the aorta in infancy with the appreciable risk of recoarctation, until more long-term follow-up results are known. The patients should not be lost to follow-up."}
{"id": "PMID:477703", "title": "Canrenoate--a spironolactone metabolite. Acute cardiac effects in digitalized patients.", "content": "Canrenoate (Soldactone)--a spironolactone metabolite for intravenous use--has recently been suggested as a specific antidote in patients suffering from digitalis intoxication. Theoretically, this would imply that canrenoate could counteract the effect of digoxin. The influence of canrenoate on inotropy in 12 digitalized patients was investigated by measurement of sytolic time intervals. Canrenoate improved the left ventricular contractility, since both preejection period (PEP) and the ratio between PEP and left ventricular ejection time (PEP/LVET) decreased significantly. The maximal changes in controlled atrial fibrillation, canrenoate had no effect on ventricular rate, whereas atropine increased the ventricular rate significantly.", "contents": "Canrenoate--a spironolactone metabolite. Acute cardiac effects in digitalized patients. Canrenoate (Soldactone)--a spironolactone metabolite for intravenous use--has recently been suggested as a specific antidote in patients suffering from digitalis intoxication. Theoretically, this would imply that canrenoate could counteract the effect of digoxin. The influence of canrenoate on inotropy in 12 digitalized patients was investigated by measurement of sytolic time intervals. Canrenoate improved the left ventricular contractility, since both preejection period (PEP) and the ratio between PEP and left ventricular ejection time (PEP/LVET) decreased significantly. The maximal changes in controlled atrial fibrillation, canrenoate had no effect on ventricular rate, whereas atropine increased the ventricular rate significantly."}
{"id": "PMID:477704", "title": "An experimental model for angina pectoris using krypton-81m. The dynamic balance between myocardial perfusion and metabolism.", "content": "16 anesthetized and open chest dogs were studied. Regional myocardial perfusion was assessed using a constant infusion of krypton-81m (half-life 13 sec) into the aortic sinuses and a gamma camera linked to a digital computer. The epicardial electrocardiogram was recorded and the plasma activity of creatine kinase was measured in serial blood samples from the aorta and a local coronary vein draining the area of myocardium supplied by the left anterior descending coronary artery (LAD). These parameters were observed throughout the whole period of a 5-h experiment. Two reversible snares were positioned on the middle portion of this artery. A critical narrowing of this vessel was produced and a peripheral venous infusion of isoproterenol (causing a 5--10% increase in heart rate and a 10--15% fall in blood pressure) was used to increase myocardial oxygen demand. During infusion there was both a relative and absolute fall in regional myocardial perfusion together with evidence of myocardial ischemia in the epicardial electrocardiogram. Provided the infusion was discontinued within 30 min (8 dogs) myocardial perfusion and the epicardial electrocardiogram returned to normal during a 5-h recovery period. In addition there was no efflux of creatine kinase activity from the ischemic area. When infusion was continued for 1 h (4 dogs) permanent alterations in myocardial perfusion and the epicardial electrocardiogram occurred and there was increased creatine kinase activity released from the area of myocardium by the narrowed vessel. Infusion for 40 min in 4 dogs produced permanent alterations in the parameters measured in 2 and complete recovery in the remaining 2. A further 4 dogs were studied in the same way but without a snare on the coronary artery. Isoproterenol given for 1 h produced no effects on any of the parameters either during or after infusion.", "contents": "An experimental model for angina pectoris using krypton-81m. The dynamic balance between myocardial perfusion and metabolism. 16 anesthetized and open chest dogs were studied. Regional myocardial perfusion was assessed using a constant infusion of krypton-81m (half-life 13 sec) into the aortic sinuses and a gamma camera linked to a digital computer. The epicardial electrocardiogram was recorded and the plasma activity of creatine kinase was measured in serial blood samples from the aorta and a local coronary vein draining the area of myocardium supplied by the left anterior descending coronary artery (LAD). These parameters were observed throughout the whole period of a 5-h experiment. Two reversible snares were positioned on the middle portion of this artery. A critical narrowing of this vessel was produced and a peripheral venous infusion of isoproterenol (causing a 5--10% increase in heart rate and a 10--15% fall in blood pressure) was used to increase myocardial oxygen demand. During infusion there was both a relative and absolute fall in regional myocardial perfusion together with evidence of myocardial ischemia in the epicardial electrocardiogram. Provided the infusion was discontinued within 30 min (8 dogs) myocardial perfusion and the epicardial electrocardiogram returned to normal during a 5-h recovery period. In addition there was no efflux of creatine kinase activity from the ischemic area. When infusion was continued for 1 h (4 dogs) permanent alterations in myocardial perfusion and the epicardial electrocardiogram occurred and there was increased creatine kinase activity released from the area of myocardium by the narrowed vessel. Infusion for 40 min in 4 dogs produced permanent alterations in the parameters measured in 2 and complete recovery in the remaining 2. A further 4 dogs were studied in the same way but without a snare on the coronary artery. Isoproterenol given for 1 h produced no effects on any of the parameters either during or after infusion."}
{"id": "PMID:477705", "title": "Effect of respiration on echocardiographic ventricular dimensions and relationship to the second heart sound.", "content": "The effects of normal respiration on echocardiographic right and left ventricular dimensions were studied in 8 normal subjects (group 1) and in 10 patients with ventricular volume overload due to anaemia (group 2). Right ventricular internal diameter (RVID) increased on inspiration in both groups of patients (group 1, 0.3 +/- 0.1 cm; group 2, 0.6 +/- 0.4 cm), while left ventricular internal diameter (LVID) decreased (group 1, 0.3 +/- 0.2 cm; group 2, 0.4 +/- 0.3 cm). The changes in echocardiographic diameter were related to the duration of electromechanical systole and to the changes in the splitting of the second heart sound; there was a linear relationship between RVID and Q--P2 (P less than 0.001) and A2--P2 (P less than 0.001). Echocardiographic measurements of cardiac chamber size should take into account the changes which occur during respiration.", "contents": "Effect of respiration on echocardiographic ventricular dimensions and relationship to the second heart sound. The effects of normal respiration on echocardiographic right and left ventricular dimensions were studied in 8 normal subjects (group 1) and in 10 patients with ventricular volume overload due to anaemia (group 2). Right ventricular internal diameter (RVID) increased on inspiration in both groups of patients (group 1, 0.3 +/- 0.1 cm; group 2, 0.6 +/- 0.4 cm), while left ventricular internal diameter (LVID) decreased (group 1, 0.3 +/- 0.2 cm; group 2, 0.4 +/- 0.3 cm). The changes in echocardiographic diameter were related to the duration of electromechanical systole and to the changes in the splitting of the second heart sound; there was a linear relationship between RVID and Q--P2 (P less than 0.001) and A2--P2 (P less than 0.001). Echocardiographic measurements of cardiac chamber size should take into account the changes which occur during respiration."}
{"id": "PMID:477707", "title": "Effect of nifedipine on plasma renin, aldosterone and catecholamines in arterial hypertension.", "content": "Acute sublingual administration of nifedipine 10--20 mg to 13 hypertensive patients caused a rapid decrease in blood pressure (BP) and a concomitant increase in heart rate (HR), plasma noradrenaline (NA) and plasma renin activity (PRA); there was no significant change in plasma adrenaline (A) or aldosterone (ALDO). Basal PRA was the major determinant of the rise in PRA, as a close correlation was present between the basal value and the increase caused by nifedipine (r = 0.92), p less than 0.001). The rise in PRA was also correlated with the plasma concentration of nifedipine after 60 min (r = 0.80, p less than 0.01), but it was not correlated with the decrease in BP, the rise in HR or the increase in NA. Nifedipine 30--60 mg daily for 6 weeks caused a reduction in mean BP from 133 to 113 mmHg (p less than 0.001). Body weight and serum potassium decreased but no consistent change was noted in NA, PRA, ALDO or 24 h-excretion of catecholamines. A significant correlation was present between the change in NA and that in PRA (r = 0.74, p less than 0.01). The alterations in the various parameters in the acute and chronic studies were not correlated. The findings indicate that different regulatory mechanisms are activated during acute and chronic administration of nifedipine. It is suggested that an initial rise in sympathetic activity gradually decreases during prolonged therapy, but it still remains a determinant of PRA.", "contents": "Effect of nifedipine on plasma renin, aldosterone and catecholamines in arterial hypertension. Acute sublingual administration of nifedipine 10--20 mg to 13 hypertensive patients caused a rapid decrease in blood pressure (BP) and a concomitant increase in heart rate (HR), plasma noradrenaline (NA) and plasma renin activity (PRA); there was no significant change in plasma adrenaline (A) or aldosterone (ALDO). Basal PRA was the major determinant of the rise in PRA, as a close correlation was present between the basal value and the increase caused by nifedipine (r = 0.92), p less than 0.001). The rise in PRA was also correlated with the plasma concentration of nifedipine after 60 min (r = 0.80, p less than 0.01), but it was not correlated with the decrease in BP, the rise in HR or the increase in NA. Nifedipine 30--60 mg daily for 6 weeks caused a reduction in mean BP from 133 to 113 mmHg (p less than 0.001). Body weight and serum potassium decreased but no consistent change was noted in NA, PRA, ALDO or 24 h-excretion of catecholamines. A significant correlation was present between the change in NA and that in PRA (r = 0.74, p less than 0.01). The alterations in the various parameters in the acute and chronic studies were not correlated. The findings indicate that different regulatory mechanisms are activated during acute and chronic administration of nifedipine. It is suggested that an initial rise in sympathetic activity gradually decreases during prolonged therapy, but it still remains a determinant of PRA."}
{"id": "PMID:477708", "title": "Intracardiac electrophysiological effects of lorcainide in man.", "content": "The electrophysiological effects of lorcainide 1.25 or 2.5 mg/kg given iv over 2 or 4 min, were studied in 21 patients with normal and diseased impulse formation and conduction, by means of intracardiac recording and stimulation. Sinus rate and the effective atrial refractory period rose following both doses of lorcainide. The corrected sinus node recovery time rose only after lorcainide 2.5 mg/kg and then most markedly in patients with sinus node dysfunction. The P-A interval remained unchanged following the drug. The A-H interval during sinus rhythm, and the pooled A-H intervals during atrial pacing, increased slightly, and the functional and effective A-V nodal refactory period changed variably. Wenckebach periods above the bundle of His occurred at lower atrial pacing rates following both doses of lorcainide in 7 patients, at the same atrial pacing rate in 9 and at higher rates in 3. H-V intervals, pooled H-V intervals and QRS-width lengthened in all patients, most markedly in cases with a conduction delay below the His bundle, who had received lorcainide 2.5 mg/kg. Thus, lorcainide shares some electrophysiological properties with procainamide and aprindine. Higher doses should be used with caution in patients with pre-existing conduction delay below the bundle His.", "contents": "Intracardiac electrophysiological effects of lorcainide in man. The electrophysiological effects of lorcainide 1.25 or 2.5 mg/kg given iv over 2 or 4 min, were studied in 21 patients with normal and diseased impulse formation and conduction, by means of intracardiac recording and stimulation. Sinus rate and the effective atrial refractory period rose following both doses of lorcainide. The corrected sinus node recovery time rose only after lorcainide 2.5 mg/kg and then most markedly in patients with sinus node dysfunction. The P-A interval remained unchanged following the drug. The A-H interval during sinus rhythm, and the pooled A-H intervals during atrial pacing, increased slightly, and the functional and effective A-V nodal refactory period changed variably. Wenckebach periods above the bundle of His occurred at lower atrial pacing rates following both doses of lorcainide in 7 patients, at the same atrial pacing rate in 9 and at higher rates in 3. H-V intervals, pooled H-V intervals and QRS-width lengthened in all patients, most markedly in cases with a conduction delay below the His bundle, who had received lorcainide 2.5 mg/kg. Thus, lorcainide shares some electrophysiological properties with procainamide and aprindine. Higher doses should be used with caution in patients with pre-existing conduction delay below the bundle His."}
{"id": "PMID:477709", "title": "Why are psychotropic drugs prescribed to out-patients? A methodological study.", "content": "The prescription of psychotropic drugs at a multidoctor district health centre in northern Sweden in 1973, was analysed by means of problem-oriented medical records. Of the 22,000 inhabitants of the district 10,700 consulted the health centre. Psychotropic drugs were prescribed for 11.3% of the patients, corresponding to 5% of the inhabitants of the area. Sixty per cent of the patients received one psychotropic prescription and 90% not more than three. Two-thirds of prescriptions were for women. Hypnotics, sedatives and minor tranquillisers constituted 64% of all prescriptions, major tranquillisers 24% and antidepressants 12%. One fifth of the patients obtained drugs belonging to more than one of the major psychotropic groups during the year. Insomnia, psychoneurosis and depression made up two-thrids of the indications for psychotropic drug therapy. More than thirty different psychotropic drugs were prescribed for the two major indications. There was considerable variation in how the different doctors prescribed drugs for the same indication. Fifty-nine different drug products were prescribed, of which the commonest five constituted more than half of the total number. Individual doctors used from 22 to 38 different psychotropic drugs.", "contents": "Why are psychotropic drugs prescribed to out-patients? A methodological study. The prescription of psychotropic drugs at a multidoctor district health centre in northern Sweden in 1973, was analysed by means of problem-oriented medical records. Of the 22,000 inhabitants of the district 10,700 consulted the health centre. Psychotropic drugs were prescribed for 11.3% of the patients, corresponding to 5% of the inhabitants of the area. Sixty per cent of the patients received one psychotropic prescription and 90% not more than three. Two-thirds of prescriptions were for women. Hypnotics, sedatives and minor tranquillisers constituted 64% of all prescriptions, major tranquillisers 24% and antidepressants 12%. One fifth of the patients obtained drugs belonging to more than one of the major psychotropic groups during the year. Insomnia, psychoneurosis and depression made up two-thrids of the indications for psychotropic drug therapy. More than thirty different psychotropic drugs were prescribed for the two major indications. There was considerable variation in how the different doctors prescribed drugs for the same indication. Fifty-nine different drug products were prescribed, of which the commonest five constituted more than half of the total number. Individual doctors used from 22 to 38 different psychotropic drugs."}
{"id": "PMID:477710", "title": "Blood level of cimetidine in relation to age.", "content": "The blood level versus time curve for unchanged cimetidine after a 200 mg oral dose has been determined in 20 apparently healthy subjects, ranging from 22 to 84 years of age. A significant relationship between the area under the curve (AUC) and age was found (r = 0.81; P less than 0.001). The peak concentrations of cimetidine were significantly inversely related to body weight (r = -0.71; P less than 0.001). The age-related increase in bioavailability of oral cimetidine, as measured by AUC, was probably due to decreased total clearance of the drug, which resulted from the opposed changes (by themselves not significant) of distribution volume towards a decrease and of half-life towards an increase with age. Reduction in the standard oral dose of cimetidine by one third to one half should be feasible in the elderly without loss of efficacy, and it may be advisable in order to obviate extreme individual responses that may occur in this population.", "contents": "Blood level of cimetidine in relation to age. The blood level versus time curve for unchanged cimetidine after a 200 mg oral dose has been determined in 20 apparently healthy subjects, ranging from 22 to 84 years of age. A significant relationship between the area under the curve (AUC) and age was found (r = 0.81; P less than 0.001). The peak concentrations of cimetidine were significantly inversely related to body weight (r = -0.71; P less than 0.001). The age-related increase in bioavailability of oral cimetidine, as measured by AUC, was probably due to decreased total clearance of the drug, which resulted from the opposed changes (by themselves not significant) of distribution volume towards a decrease and of half-life towards an increase with age. Reduction in the standard oral dose of cimetidine by one third to one half should be feasible in the elderly without loss of efficacy, and it may be advisable in order to obviate extreme individual responses that may occur in this population."}
{"id": "PMID:477711", "title": "Influence of food on the absorption of phenytoin in man.", "content": "The influence of food intake on the absorption of phenytoin was examined in eight healthy volunteers, by study of single-dose kinetics following ingestion of phenytoin 300 mg either with a standardized breakfast or on an empty stomach. Blood samples were collected at regular intervals from 0 to 48 h, and serum concentrations of unmetabolized phenytoin were determined by gas chromatography. Serum concentrations of the major metabolite of phenytoin, 4-hydroxyphenytoin, were measured by mass fragmentography. Concurrent intake of food and phenytoin appeared to accelerate absorption of the drug from the formulation used, and the peak concentrations were significantly higher (mean increase 40%) in the postprandial than in the preprandial state. As reflected by the AUC (area under the curve), the amount of drug absorbed was increased during postprandial conditions, although the difference only reached borderline significance. It is suggested that phenytoin should always be taken in a defined relation to meals.", "contents": "Influence of food on the absorption of phenytoin in man. The influence of food intake on the absorption of phenytoin was examined in eight healthy volunteers, by study of single-dose kinetics following ingestion of phenytoin 300 mg either with a standardized breakfast or on an empty stomach. Blood samples were collected at regular intervals from 0 to 48 h, and serum concentrations of unmetabolized phenytoin were determined by gas chromatography. Serum concentrations of the major metabolite of phenytoin, 4-hydroxyphenytoin, were measured by mass fragmentography. Concurrent intake of food and phenytoin appeared to accelerate absorption of the drug from the formulation used, and the peak concentrations were significantly higher (mean increase 40%) in the postprandial than in the preprandial state. As reflected by the AUC (area under the curve), the amount of drug absorbed was increased during postprandial conditions, although the difference only reached borderline significance. It is suggested that phenytoin should always be taken in a defined relation to meals."}
{"id": "PMID:477712", "title": "Pharmacokinetics of bromocriptine during continuous oral treatment of Parkinson's disease.", "content": "The plasma kinetics of bromocriptine (BCT), a long-acting dopamine agonist, was studied in twelve patients with Parkinson's disease, using a newly developed gas chromatographic method of analysis. Each patient received BCT for at least three weeks in a constant but different dose regimen. Concomitant treatment with 1-DOPA was not allowed. During a 6-day hospitalization period, a blood sample was taken immediately before the afternoon dose at 14.00 h (Cmin) to determine the steady-state level. On the 6th day blood samples were collected every hour during two 8 h dose intervals. The results showed a significant correlation between the mean values of the AUC and the Cmin. First order elimination kinetics appeared to be followed by BCT, at least for the plasma concentrations commonly found. Considerable inter-individual variation was demonstrated both for the dose/plasma concentration ratio and for calculated plasma clearances. No serious side-effects were observed during the investigation.", "contents": "Pharmacokinetics of bromocriptine during continuous oral treatment of Parkinson's disease. The plasma kinetics of bromocriptine (BCT), a long-acting dopamine agonist, was studied in twelve patients with Parkinson's disease, using a newly developed gas chromatographic method of analysis. Each patient received BCT for at least three weeks in a constant but different dose regimen. Concomitant treatment with 1-DOPA was not allowed. During a 6-day hospitalization period, a blood sample was taken immediately before the afternoon dose at 14.00 h (Cmin) to determine the steady-state level. On the 6th day blood samples were collected every hour during two 8 h dose intervals. The results showed a significant correlation between the mean values of the AUC and the Cmin. First order elimination kinetics appeared to be followed by BCT, at least for the plasma concentrations commonly found. Considerable inter-individual variation was demonstrated both for the dose/plasma concentration ratio and for calculated plasma clearances. No serious side-effects were observed during the investigation."}
{"id": "PMID:477714", "title": "Excretion of hydroflumethiazide in bile and urine of man.", "content": "Biliary and urinary excretion of hydroflumethiazide (HFT) and its metabolite, 2,4-disulfamyl-5-trifluoromethylaniline (DTA), was investigated in 5 otherwise healthy patients with a T-drain in the common bile duct after cholecystectomy and choledochotomy. After a single oral dose of HFT 302--453 micromoles, a mean of 0.051% (range 0.028--0.075) of the dose was excreted in bile and 34.9% (range 23.7--45.4) in urine during the first 6 hours after administration. Biliary excretion appeared to be of minor importance in the elimination of HFT by man. DTA could not be detected in bile.", "contents": "Excretion of hydroflumethiazide in bile and urine of man. Biliary and urinary excretion of hydroflumethiazide (HFT) and its metabolite, 2,4-disulfamyl-5-trifluoromethylaniline (DTA), was investigated in 5 otherwise healthy patients with a T-drain in the common bile duct after cholecystectomy and choledochotomy. After a single oral dose of HFT 302--453 micromoles, a mean of 0.051% (range 0.028--0.075) of the dose was excreted in bile and 34.9% (range 23.7--45.4) in urine during the first 6 hours after administration. Biliary excretion appeared to be of minor importance in the elimination of HFT by man. DTA could not be detected in bile."}
{"id": "PMID:477716", "title": "An opposing role for the adrenals in the hypotensive effects of propranolol in the spontaneously hypertensive rat.", "content": "d,l-Propranolol (1 and 5 mg/kg s.c.) did not cause a fall in blood pressure and induced only a limited decrease in heart rate in conscious spontaneously) hypertensive rats (SHR). In contrast, after bilateral adrenalectomy, d,l-propranolol induced a rapid and profound decrease in blood pressure and heart rate. Decreases in heart rate and blood pressure in the individual animals were not correlated. The effects were mainly caused by l-propranolol but an additional effect of d-propranolol cannot be excluded. The decrease in blood pressure was not observed after removal of the adrenal medulla. Heart rate decreased only slightly in these animals. After treatment of adrenalectomized SHR with corticosterone (1 mg/kg b.w./h) the decrease in blood pressure due to d,l-propranolol was completely abolished. The fall in heart rate was diminished. Central injection of d,l-propranolol into the lateral brain ventricle of adrenalectomized SHR caused cardiovascular changes which were less pronounced than those following peripheral injection of comparable doses. The inhibitory effects of d,l-propranolol also occurred in adrenalectomized normotensive Wistar Kyoto rats. However, no significant changes in blood pressure and only a limited fall in heart rate were observed in adrenalectomized normotensive and renal hypertensive Wistar rats. It is concluded that the presence of the adrenal cortex, but not of the adrenal medulla prevents acute hypotension and bradycardia after propranolol in the conscious SHR.", "contents": "An opposing role for the adrenals in the hypotensive effects of propranolol in the spontaneously hypertensive rat. d,l-Propranolol (1 and 5 mg/kg s.c.) did not cause a fall in blood pressure and induced only a limited decrease in heart rate in conscious spontaneously) hypertensive rats (SHR). In contrast, after bilateral adrenalectomy, d,l-propranolol induced a rapid and profound decrease in blood pressure and heart rate. Decreases in heart rate and blood pressure in the individual animals were not correlated. The effects were mainly caused by l-propranolol but an additional effect of d-propranolol cannot be excluded. The decrease in blood pressure was not observed after removal of the adrenal medulla. Heart rate decreased only slightly in these animals. After treatment of adrenalectomized SHR with corticosterone (1 mg/kg b.w./h) the decrease in blood pressure due to d,l-propranolol was completely abolished. The fall in heart rate was diminished. Central injection of d,l-propranolol into the lateral brain ventricle of adrenalectomized SHR caused cardiovascular changes which were less pronounced than those following peripheral injection of comparable doses. The inhibitory effects of d,l-propranolol also occurred in adrenalectomized normotensive Wistar Kyoto rats. However, no significant changes in blood pressure and only a limited fall in heart rate were observed in adrenalectomized normotensive and renal hypertensive Wistar rats. It is concluded that the presence of the adrenal cortex, but not of the adrenal medulla prevents acute hypotension and bradycardia after propranolol in the conscious SHR."}
{"id": "PMID:477718", "title": "Inhibition of gastric acid secretion by a glycoprotein isolated from human urine (human urinary gastric inhibitor).", "content": "The gastric antisecretory activity of an inhibitor newly isolated from human urine (Human Urinary Gastric Inhibitor or HUGI) has been studied. HUGI was given intravenously and its activity determined in the following test systems: gastric secretion in the rat with pyloric ligation; gastric secretion in the dog with a Heidenhain pouch stimulated with pentagastrin, histamine and a protein meal; acid secretion by the isolated gastric mucosa of the rat; gastrointestinal motility; bile flow and gall-bladder tone and arterial and venous blood pressure and heart rate. HUGI was found to have marked activity only in the pyloric-ligated rats and in the dogs with Heidenhain pouches stimulated by a protein meal. Particularly in the dog, HUGI (0.1 to 6.4 micrograms/kg, i.v) markedly inhibited gastric secretion, dose-dependently and without changing the plasma gastrin concentration. Negative results were obtained in the other tests, but these results serve to demonstrate that HUGI is an inhibitor well-differentiated from other glycoproteins or peptides with gastric antisecretory activity, such as urogastrone and GIP. The results obtained to date are not sufficient to allow the mechanism of action of HUGI to be defined.", "contents": "Inhibition of gastric acid secretion by a glycoprotein isolated from human urine (human urinary gastric inhibitor). The gastric antisecretory activity of an inhibitor newly isolated from human urine (Human Urinary Gastric Inhibitor or HUGI) has been studied. HUGI was given intravenously and its activity determined in the following test systems: gastric secretion in the rat with pyloric ligation; gastric secretion in the dog with a Heidenhain pouch stimulated with pentagastrin, histamine and a protein meal; acid secretion by the isolated gastric mucosa of the rat; gastrointestinal motility; bile flow and gall-bladder tone and arterial and venous blood pressure and heart rate. HUGI was found to have marked activity only in the pyloric-ligated rats and in the dogs with Heidenhain pouches stimulated by a protein meal. Particularly in the dog, HUGI (0.1 to 6.4 micrograms/kg, i.v) markedly inhibited gastric secretion, dose-dependently and without changing the plasma gastrin concentration. Negative results were obtained in the other tests, but these results serve to demonstrate that HUGI is an inhibitor well-differentiated from other glycoproteins or peptides with gastric antisecretory activity, such as urogastrone and GIP. The results obtained to date are not sufficient to allow the mechanism of action of HUGI to be defined."}
{"id": "PMID:477719", "title": "3,4-Dihydroxyphenylacetic acid and homovanillic acid in rat plasma: possible indicators of central dopaminergic activity.", "content": "The concentrations of dopamine (DA) metabolites (free and conjugated) was measured in plasma and brain regions of rats by the mass spectrometric method of selected ion monitoring. Experimental treatments which altered the function of central dopamine neurons also induced concomitant changes in plasma 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA). Stimulation of the nigrostriatal pathway increased plasma DOPAC and HVA whereas lesion of the pathway decreased plasma metabolites. Several drug treatments induced parallel changes in brain and plasma concentrations of DA metabolites. It is suggested that changes in the concentration of DOPAC and HVA in rat brain are reflected by parallel changes in plasma. No conjugated forms of DOPAC and HVA were found in plasma and brain tissue of vervet monkeys (Cercopithecus aethiops).", "contents": "3,4-Dihydroxyphenylacetic acid and homovanillic acid in rat plasma: possible indicators of central dopaminergic activity. The concentrations of dopamine (DA) metabolites (free and conjugated) was measured in plasma and brain regions of rats by the mass spectrometric method of selected ion monitoring. Experimental treatments which altered the function of central dopamine neurons also induced concomitant changes in plasma 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA). Stimulation of the nigrostriatal pathway increased plasma DOPAC and HVA whereas lesion of the pathway decreased plasma metabolites. Several drug treatments induced parallel changes in brain and plasma concentrations of DA metabolites. It is suggested that changes in the concentration of DOPAC and HVA in rat brain are reflected by parallel changes in plasma. No conjugated forms of DOPAC and HVA were found in plasma and brain tissue of vervet monkeys (Cercopithecus aethiops)."}
{"id": "PMID:477720", "title": "Mobilization of stored calcium for phasic contraction induced by norepinephrine in rabbit aorta.", "content": "In Ca-free solution norepinephrine (NE) produced only a phasic contraction in the media-intima layer of rabbit aorta. The second application of NE was almost ineffective. Incubation of the muscle with Ca for a short period (Ca loading) restored the ability to produce a phasic contraction in Ca-free solution. Various ions and compounds were applied to the muscle prior to the Ca loading or prior to NE application and it was found that these treatments variously affected the NE-induced phasic contraction. The data suggest that the NE-induced phasic contraction in Ca-free solution results from Ca release from cellular sites and that a hyperbolic relationship exists between the amount of Ca at these sites and the magnitude of the contraction. These sites take up extracellular Ca by a process sensitive to La3+ but not to D600; the Ca influx stimulated by high K solution, which is sensitive to D600 and also contributes to refilling of these sites; NE releases this stored Ca by a process sensitive to procaine, caffeine and theophylline and, in this manner, elicits a phasic contraction.", "contents": "Mobilization of stored calcium for phasic contraction induced by norepinephrine in rabbit aorta. In Ca-free solution norepinephrine (NE) produced only a phasic contraction in the media-intima layer of rabbit aorta. The second application of NE was almost ineffective. Incubation of the muscle with Ca for a short period (Ca loading) restored the ability to produce a phasic contraction in Ca-free solution. Various ions and compounds were applied to the muscle prior to the Ca loading or prior to NE application and it was found that these treatments variously affected the NE-induced phasic contraction. The data suggest that the NE-induced phasic contraction in Ca-free solution results from Ca release from cellular sites and that a hyperbolic relationship exists between the amount of Ca at these sites and the magnitude of the contraction. These sites take up extracellular Ca by a process sensitive to La3+ but not to D600; the Ca influx stimulated by high K solution, which is sensitive to D600 and also contributes to refilling of these sites; NE releases this stored Ca by a process sensitive to procaine, caffeine and theophylline and, in this manner, elicits a phasic contraction."}
{"id": "PMID:477721", "title": "Analysis of the effects of bromocriptine on blood pressure and sympathetic nerve function.", "content": "Bromocriptine, a dopamine receptor agonist, lowered arterial blood pressure and caused significant impairment of the cardioacceleration observed during the stimulation of the right postganglionic cardiac sympathetic nerve fibers in pentobarbital-anethetized dogs. Prior administration of pimozide antagonized the hypotensive and the inhibitory action of bromocriptine on cardiac sympathetic function. These results suggest that while presynaptic dopamine receptors seem to be involved in mediating the inhibitory action of bromocriptine on cardiac sympathetic function, the role of presynaptic as well as dopamine receptors located at other sites in mediating the hypotensive action of bromocriptine needs to be further investigated.", "contents": "Analysis of the effects of bromocriptine on blood pressure and sympathetic nerve function. Bromocriptine, a dopamine receptor agonist, lowered arterial blood pressure and caused significant impairment of the cardioacceleration observed during the stimulation of the right postganglionic cardiac sympathetic nerve fibers in pentobarbital-anethetized dogs. Prior administration of pimozide antagonized the hypotensive and the inhibitory action of bromocriptine on cardiac sympathetic function. These results suggest that while presynaptic dopamine receptors seem to be involved in mediating the inhibitory action of bromocriptine on cardiac sympathetic function, the role of presynaptic as well as dopamine receptors located at other sites in mediating the hypotensive action of bromocriptine needs to be further investigated."}
{"id": "PMID:477722", "title": "Vasodilator response to histamine: dependence upon the site of administration.", "content": "Histamine causes vasodilation in part by interacting with histamine H2 receptors. The present study was undertaken to evaluate the difference in vasodilator sensitivity of H2 receptors on the inside compared to the outside of the vessel. In order to induce tone, norepinephrine was administered to an in vitro preparation of rabbit ear artery treated with mepyramine to block H1 receptors. Histamine was then either selectively perfused through the artery or added to the outside of the vessel via the organ bath. The outside of the artery was found to be twice as sensitive to the vasodilator effect of histamine as the inside. These data provide the first evidence for greater extraluminal sensitivity of vascular smooth muscle and suggest that the response to histamine will depend in part on the route the amine takes to reach receptors, e.g., from blood-borne sources or from extraluminal stores.", "contents": "Vasodilator response to histamine: dependence upon the site of administration. Histamine causes vasodilation in part by interacting with histamine H2 receptors. The present study was undertaken to evaluate the difference in vasodilator sensitivity of H2 receptors on the inside compared to the outside of the vessel. In order to induce tone, norepinephrine was administered to an in vitro preparation of rabbit ear artery treated with mepyramine to block H1 receptors. Histamine was then either selectively perfused through the artery or added to the outside of the vessel via the organ bath. The outside of the artery was found to be twice as sensitive to the vasodilator effect of histamine as the inside. These data provide the first evidence for greater extraluminal sensitivity of vascular smooth muscle and suggest that the response to histamine will depend in part on the route the amine takes to reach receptors, e.g., from blood-borne sources or from extraluminal stores."}
{"id": "PMID:477724", "title": "Sulpiride: a weak antagonist of norepinephrine and 5-hydroxytryptamine.", "content": "Antagonism of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) by sulpiride was studied in vitro on rabbit aortic strips. Concentrations of sulpiride ranging from 3 X 10(-5) to 3 X 10(-3) M caused a progressive shift of the dose response curves of both NE and 5-HT to the right without inhibiting the responses to KCl. pA2 values of sulpiride calculated from Schild plots against NE and 5-HT were 4.6 and 4.4, respectively. Thus, sulpiride, which has been previously shown to be the most potent antagonist of the dopamine vascular receptors, is a very weak alpha-adrenergic or 5-HT antagonist.", "contents": "Sulpiride: a weak antagonist of norepinephrine and 5-hydroxytryptamine. Antagonism of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) by sulpiride was studied in vitro on rabbit aortic strips. Concentrations of sulpiride ranging from 3 X 10(-5) to 3 X 10(-3) M caused a progressive shift of the dose response curves of both NE and 5-HT to the right without inhibiting the responses to KCl. pA2 values of sulpiride calculated from Schild plots against NE and 5-HT were 4.6 and 4.4, respectively. Thus, sulpiride, which has been previously shown to be the most potent antagonist of the dopamine vascular receptors, is a very weak alpha-adrenergic or 5-HT antagonist."}
{"id": "PMID:477723", "title": "Enhancement of in vitro binding and some of the pharmacological properties of diazepam by a novel anthelmintic agent, Avermectin B1a.", "content": "A novel macrocyclic lactone disaccharide anthelmintic agent, Avermectin B1a (AVM) has been found to cause a concentration-dependent increase in the in vitro binding of 3H-diazepam to rat and mouse brain membranes. The increase in binding is manifested as both an increase in the affinity and number of bindings sites for 3H-diazepam. Preliminary in vivo studies demonstrate that AVM can also enhance some of the pharmacological actions of diazepam.", "contents": "Enhancement of in vitro binding and some of the pharmacological properties of diazepam by a novel anthelmintic agent, Avermectin B1a. A novel macrocyclic lactone disaccharide anthelmintic agent, Avermectin B1a (AVM) has been found to cause a concentration-dependent increase in the in vitro binding of 3H-diazepam to rat and mouse brain membranes. The increase in binding is manifested as both an increase in the affinity and number of bindings sites for 3H-diazepam. Preliminary in vivo studies demonstrate that AVM can also enhance some of the pharmacological actions of diazepam."}
{"id": "PMID:477725", "title": "Cooperative interactions at [3H]kainic acid binding sites in rat and human cerebellum.", "content": "Inhibition of the specific binding of [3H]kainic acid was studied in membranes isolated from rat and human cerebellum; the sequence of potencies in both species were: kainic acid greater than L-glutamic acid greater than dihydrokainic acid greater than D-glutamic acid. Whereas the Hill coefficient for unlabelled Kainate was 1.0, dihydrokainic acid and D- and L-glutamic acids exhibited negative cooperativity with Hill coefficients of near 0.5. This allosteric interaction of glutamic acid at the kainic acid recognition site suggests a biochemical correlate for the synergistic effects of these compounds in vivo.", "contents": "Cooperative interactions at [3H]kainic acid binding sites in rat and human cerebellum. Inhibition of the specific binding of [3H]kainic acid was studied in membranes isolated from rat and human cerebellum; the sequence of potencies in both species were: kainic acid greater than L-glutamic acid greater than dihydrokainic acid greater than D-glutamic acid. Whereas the Hill coefficient for unlabelled Kainate was 1.0, dihydrokainic acid and D- and L-glutamic acids exhibited negative cooperativity with Hill coefficients of near 0.5. This allosteric interaction of glutamic acid at the kainic acid recognition site suggests a biochemical correlate for the synergistic effects of these compounds in vivo."}
{"id": "PMID:477729", "title": "Vasopressin analogs: sedative properties and passive avoidance behavior in rats.", "content": "The effects of several types of vasopressin analogs that are considered to be resistant to some of the physiologically significant enzymatic systems were investigated utilizing rats trained in a passive avoidance task. Enhancement of avoidance latencies was observed 2, 7 and 13 days after the single learning trial when deamino-carbavasopressins, triglycyl-8-lysine-vasopressin or its des-glycinamide derivative, and deamino-D-arginine-vasopressin were given shortly after the learning trial in the dose of 1 microgram s.c. (8-L-Arginine)deamino-6-carba-vasopressin and (8-L-ornithine)deamino-6-carba-vasopressin were also active in the dose of 0.1 microgram. Lysine vasopressin and its des-glycinamide derivative failed to enhance avoidance latencies in part of the experiments if doses of 0.3--3 micrograms were administered and 7 or 13 day intervals were used between the learning and the test trials. Enhancement of avoidance latencies was also observed, if some of the peptides were injected 20 min but not 120 or 180 min before the test trial. Marked depression of exploratory behavior of rats in an open field was found after s.c. injections of low doses (1--3 micrograms kg-1) of deamino-carba-vasopressins. Higher doses (10--30 micrograms kg-1) induced sleep-like immobility not accompanied by ataxia or catalepsy.", "contents": "Vasopressin analogs: sedative properties and passive avoidance behavior in rats. The effects of several types of vasopressin analogs that are considered to be resistant to some of the physiologically significant enzymatic systems were investigated utilizing rats trained in a passive avoidance task. Enhancement of avoidance latencies was observed 2, 7 and 13 days after the single learning trial when deamino-carbavasopressins, triglycyl-8-lysine-vasopressin or its des-glycinamide derivative, and deamino-D-arginine-vasopressin were given shortly after the learning trial in the dose of 1 microgram s.c. (8-L-Arginine)deamino-6-carba-vasopressin and (8-L-ornithine)deamino-6-carba-vasopressin were also active in the dose of 0.1 microgram. Lysine vasopressin and its des-glycinamide derivative failed to enhance avoidance latencies in part of the experiments if doses of 0.3--3 micrograms were administered and 7 or 13 day intervals were used between the learning and the test trials. Enhancement of avoidance latencies was also observed, if some of the peptides were injected 20 min but not 120 or 180 min before the test trial. Marked depression of exploratory behavior of rats in an open field was found after s.c. injections of low doses (1--3 micrograms kg-1) of deamino-carba-vasopressins. Higher doses (10--30 micrograms kg-1) induced sleep-like immobility not accompanied by ataxia or catalepsy."}
{"id": "PMID:477730", "title": "Effects of chlorpromazine and some of its metabolites on the EEG and on dopamine metabolism of the isolated perfused rat brain.", "content": "The study concerned the effects of chlorpromazine (CPZ), monodesmethyl-chlorpromazine (NOR1-CPZ), didesmethyl-chlorpromazine (NOR2-CPZ), and chlorpromazine-N-oxide (CPZ-NO) on the EEG and on dopamine metabolism of the isolated perfused rat brain. Isolated brains were perfused with 100 ml of a perfusion medium containing 30% bovine red cells (v/v), 2 g bovine serum albumin, 14 mM glucose as well as one of the agents in a concentration of 10 micrometers. The main dopamine metabolite homovanillic acid (HVA) was measured fluorimetrically in the striatum of the isolated brain. The EEG was recorded by two symmetrical bipolar leads from the parietal regions at various times during the 30 min perfusion period and was stored on magnetic tape. The recordings were evaluated visually and quantitatively by automatic analysis. CPZ-NO was found to be the most active agent both in changing the EEG and in elevating the HVA level in the striatum. The mean EEG amplitude and the slow wave activity increased significantly. The increase of the HVA level in the striatum was correlated with the increase of delta waves as well as excess of kurtosis and skewness calculated from the amplitude histography data. The desmethylated metabolites caused only moderate central effects.", "contents": "Effects of chlorpromazine and some of its metabolites on the EEG and on dopamine metabolism of the isolated perfused rat brain. The study concerned the effects of chlorpromazine (CPZ), monodesmethyl-chlorpromazine (NOR1-CPZ), didesmethyl-chlorpromazine (NOR2-CPZ), and chlorpromazine-N-oxide (CPZ-NO) on the EEG and on dopamine metabolism of the isolated perfused rat brain. Isolated brains were perfused with 100 ml of a perfusion medium containing 30% bovine red cells (v/v), 2 g bovine serum albumin, 14 mM glucose as well as one of the agents in a concentration of 10 micrometers. The main dopamine metabolite homovanillic acid (HVA) was measured fluorimetrically in the striatum of the isolated brain. The EEG was recorded by two symmetrical bipolar leads from the parietal regions at various times during the 30 min perfusion period and was stored on magnetic tape. The recordings were evaluated visually and quantitatively by automatic analysis. CPZ-NO was found to be the most active agent both in changing the EEG and in elevating the HVA level in the striatum. The mean EEG amplitude and the slow wave activity increased significantly. The increase of the HVA level in the striatum was correlated with the increase of delta waves as well as excess of kurtosis and skewness calculated from the amplitude histography data. The desmethylated metabolites caused only moderate central effects."}
{"id": "PMID:477731", "title": "Mechanism of the depressor response to dopamine in the rat treated with phenoxybenzamine.", "content": "Alterations of hypotensive responses to dopamine by antagonists were characterized in alpha-blocked, anaesthetized rats. Responses were not affected by d-propranolol (0.1 mg/kg) whereas d,1-propranolol (0.1 mg/kg) or haloperidol (1.0 mg/kg) attenuated them; higher doses of inhibitors (1.0 mg/kg; 5.0 mg/kg, respectively) failed to produce a higher inhibition, but combinations of low doses abolished the depressor responses. In adrenalectomized rats, hypotensive responses decreased; haloperidol always attenuated the responses while d,1-propranolol became ineffective. Dopamine produced an enhancement of plasma adrenaline and noradrenaline levels, which was decreased by d,1-propranolol and increased by haloperidol. The data suggest that in rats the depressor component of dopamine is due to activation of both dopaminergic and beta-adrenoceptors. The beta component appears to be due to the release of adrenaline. The results also support the concept of the existence in sympathetic nerve endings and adrenal glands of stimulatory beta-adrenergic and inhibitory dopaminergic prejunctional receptors.", "contents": "Mechanism of the depressor response to dopamine in the rat treated with phenoxybenzamine. Alterations of hypotensive responses to dopamine by antagonists were characterized in alpha-blocked, anaesthetized rats. Responses were not affected by d-propranolol (0.1 mg/kg) whereas d,1-propranolol (0.1 mg/kg) or haloperidol (1.0 mg/kg) attenuated them; higher doses of inhibitors (1.0 mg/kg; 5.0 mg/kg, respectively) failed to produce a higher inhibition, but combinations of low doses abolished the depressor responses. In adrenalectomized rats, hypotensive responses decreased; haloperidol always attenuated the responses while d,1-propranolol became ineffective. Dopamine produced an enhancement of plasma adrenaline and noradrenaline levels, which was decreased by d,1-propranolol and increased by haloperidol. The data suggest that in rats the depressor component of dopamine is due to activation of both dopaminergic and beta-adrenoceptors. The beta component appears to be due to the release of adrenaline. The results also support the concept of the existence in sympathetic nerve endings and adrenal glands of stimulatory beta-adrenergic and inhibitory dopaminergic prejunctional receptors."}
{"id": "PMID:477732", "title": "Comparison of binding of [3H]-methionine-enkephalin, [3H]-naltrexone and [3H]-dihydromorphine in the mouse vas deferens and the myenteric plexus and brain of the ginea pig.", "content": "[3H]-Methionine-enkephalin, [3H]-naltrexone and [3H]-dihydromorphine are specifically bound in homogenates of not only the brain and myenteric plexus of the guinea pig but also the vas deferens of the mouse. Brain has a ratio of methionine-enkephalin to dihydromorphine binding in favour of methionine-enkephalin binding and the myenteric plexus a ratio in favour of dihydromorphine binding, with the mouse vas deferens being intermediate.", "contents": "Comparison of binding of [3H]-methionine-enkephalin, [3H]-naltrexone and [3H]-dihydromorphine in the mouse vas deferens and the myenteric plexus and brain of the ginea pig. [3H]-Methionine-enkephalin, [3H]-naltrexone and [3H]-dihydromorphine are specifically bound in homogenates of not only the brain and myenteric plexus of the guinea pig but also the vas deferens of the mouse. Brain has a ratio of methionine-enkephalin to dihydromorphine binding in favour of methionine-enkephalin binding and the myenteric plexus a ratio in favour of dihydromorphine binding, with the mouse vas deferens being intermediate."}
{"id": "PMID:477733", "title": "The effects of intra-arterial bradykinin, histamine, acetylcholine and prostaglandin E1 on nociceptive and non-nociceptive dorsal horn neurones of the cat.", "content": "The effects of peripherally administered algesic agents were investigated on the firing of cat dorsal horn interneurones classified as nociceptive or non-nociceptive according to the peripheral stimuli that excited them. A small amount of bradykinin injected into the blood supplying the receptive fields of cells was a potent specific stimulus causing activation of nociceptive cells and slowly conducting nerve fibres. Larger amounts of bradykinin and large amounts of histamine, 5-hydroxytryptamine and acetylcholine activated both nociceptive and non-nociceptive cells. Prostaglandin E1 enhanced the effects of bradykinin and histamine on nociceptive cells. Prostaglandin E1 also increased the response of these cells to the application of noxious heat whilst aspirin reduced this response. These results support a chemosensitive theory of nociceptor activation and show bradykinin to be the most potent and specific of the suggested endogenous algesic agents in causing activation of CNS nociceptive pathways.", "contents": "The effects of intra-arterial bradykinin, histamine, acetylcholine and prostaglandin E1 on nociceptive and non-nociceptive dorsal horn neurones of the cat. The effects of peripherally administered algesic agents were investigated on the firing of cat dorsal horn interneurones classified as nociceptive or non-nociceptive according to the peripheral stimuli that excited them. A small amount of bradykinin injected into the blood supplying the receptive fields of cells was a potent specific stimulus causing activation of nociceptive cells and slowly conducting nerve fibres. Larger amounts of bradykinin and large amounts of histamine, 5-hydroxytryptamine and acetylcholine activated both nociceptive and non-nociceptive cells. Prostaglandin E1 enhanced the effects of bradykinin and histamine on nociceptive cells. Prostaglandin E1 also increased the response of these cells to the application of noxious heat whilst aspirin reduced this response. These results support a chemosensitive theory of nociceptor activation and show bradykinin to be the most potent and specific of the suggested endogenous algesic agents in causing activation of CNS nociceptive pathways."}
{"id": "PMID:477734", "title": "Neurotensin contracts the guinea-pig longitudinal ileal smooth muscle by inducing acetylcholine release.", "content": "Neurotensin contracted the isolated longitudinal smooth muscle strip of the guinea-pig ileum, but was only 22% as effective as histamine. Neostigmine (0.1 micrometer) increased the effectiveness of neurotensin 4-fold. Atropine (0.1 micrometer) completely inhibited the contracting effect of neurotensin measured in the presence of neostigmine, whereas hexamethonium (0.1 micrometer) was without effect. It is concluded that in the longitudinal smooth muscle of the guinea-pig ileum, the contracting effect of neurotensin is mediated by the release of acetylcholine from nerve endings in response to a postganglionic stimulation of cholinergic nerve fibers.", "contents": "Neurotensin contracts the guinea-pig longitudinal ileal smooth muscle by inducing acetylcholine release. Neurotensin contracted the isolated longitudinal smooth muscle strip of the guinea-pig ileum, but was only 22% as effective as histamine. Neostigmine (0.1 micrometer) increased the effectiveness of neurotensin 4-fold. Atropine (0.1 micrometer) completely inhibited the contracting effect of neurotensin measured in the presence of neostigmine, whereas hexamethonium (0.1 micrometer) was without effect. It is concluded that in the longitudinal smooth muscle of the guinea-pig ileum, the contracting effect of neurotensin is mediated by the release of acetylcholine from nerve endings in response to a postganglionic stimulation of cholinergic nerve fibers."}
{"id": "PMID:477737", "title": "Effects of disopyramide on SA nodal pacemaker activity and contractility in the isolated blood-perfused atrium of the dog.", "content": "The isolated blood-perfused preparations of canine atrium were suspended in a bath and perfused with arterial blood led from the carotid artery of the heparinized donor dog. Disopyramide caused dose-related negative chronotropic and inotropic effects in a dose range of 30-1000 microgram when injected directly into the cannulated sinus node artery of the isolated atrium. The order of potencies for inducing the negative chronotropic effect in isolated atrium preparations was verapamil greater than propranolol greater than lidocaine = quinidine greater than phenytoin greater than or equal to disopyramide greater than procainamide. On the other hand, the order of potencies for inducing the negative inotropic effect was verapamil = propranolol greater than lidocaine greater than or equal to phenytoin greater than disopyramide greater than procainamide greater than or equal to quinidine. When disopyramide (1 mg/kg or 3 mg/kg) was administered i.v. into the jugular vein of the donor dog, the systemic blood pressure of the donor dog was markedly decreased. However, the tension developed and sinus rate of the isolated atrium were only slightly decreased. Disopyramide produced greater suppression at higher frequencies and slightly depressed the calcium chloride-induced positive inotropic effects.", "contents": "Effects of disopyramide on SA nodal pacemaker activity and contractility in the isolated blood-perfused atrium of the dog. The isolated blood-perfused preparations of canine atrium were suspended in a bath and perfused with arterial blood led from the carotid artery of the heparinized donor dog. Disopyramide caused dose-related negative chronotropic and inotropic effects in a dose range of 30-1000 microgram when injected directly into the cannulated sinus node artery of the isolated atrium. The order of potencies for inducing the negative chronotropic effect in isolated atrium preparations was verapamil greater than propranolol greater than lidocaine = quinidine greater than phenytoin greater than or equal to disopyramide greater than procainamide. On the other hand, the order of potencies for inducing the negative inotropic effect was verapamil = propranolol greater than lidocaine greater than or equal to phenytoin greater than disopyramide greater than procainamide greater than or equal to quinidine. When disopyramide (1 mg/kg or 3 mg/kg) was administered i.v. into the jugular vein of the donor dog, the systemic blood pressure of the donor dog was markedly decreased. However, the tension developed and sinus rate of the isolated atrium were only slightly decreased. Disopyramide produced greater suppression at higher frequencies and slightly depressed the calcium chloride-induced positive inotropic effects."}
{"id": "PMID:477738", "title": "Central mediation of the antihypertensive effect of pargyline in spontaneously hypertensive rats.", "content": "The monoamine oxidase (MAO) inhibitor pargyline induced a moderate (about 20 mm Hg) but persistent (48 h) decrease of systolic blood pressure in unanesthetized adult spontaneously hypertensive rats (SHR) but not in normotensive rats. The fall of blood pressure correlated with the blockade of norepinephrine (NE) deamination by brain homogenates. After an intracerebroventricular (icv) injection of 6-hydroxydopamine, which lowered brain NE content by about 70%, pargyline was unable to diminish arterial pressure. Blockade of central alpha-adrenoceptors by treatment with phentolamine (100 microgram icv) could either prevent or reverse the fall of blood pressure in SHR induced by pargylline. Moreover, a low dose of pargyline injected directly into the brain lowered arterial pressure. We conclude that the hypotensive action of pargylline in SHR appears to be the consequence of NE accumulating at an inhibitory alpha-adrenoceptor in brain.", "contents": "Central mediation of the antihypertensive effect of pargyline in spontaneously hypertensive rats. The monoamine oxidase (MAO) inhibitor pargyline induced a moderate (about 20 mm Hg) but persistent (48 h) decrease of systolic blood pressure in unanesthetized adult spontaneously hypertensive rats (SHR) but not in normotensive rats. The fall of blood pressure correlated with the blockade of norepinephrine (NE) deamination by brain homogenates. After an intracerebroventricular (icv) injection of 6-hydroxydopamine, which lowered brain NE content by about 70%, pargyline was unable to diminish arterial pressure. Blockade of central alpha-adrenoceptors by treatment with phentolamine (100 microgram icv) could either prevent or reverse the fall of blood pressure in SHR induced by pargylline. Moreover, a low dose of pargyline injected directly into the brain lowered arterial pressure. We conclude that the hypotensive action of pargylline in SHR appears to be the consequence of NE accumulating at an inhibitory alpha-adrenoceptor in brain."}
{"id": "PMID:477739", "title": "Physiological function of descending noradrenaline projections to the spinal cord: role in post-decapitation convulsions.", "content": "Destruction of the descending noradrenergic innervation to the spinal cord, but not that to the cerebellum or the forebrain, by the use of intracerebral injection of 6-hydroxydopamine completely prevented the occurrence of the usual itation convulsion. Depletion of brain noradrenaline by synthesis inhibition with DDC, FLA 57 or FLA 63 g reduced the duration of the post-decapitation convulsion. Blockade of alpha-noradrenergic receptors by phentolamine or phenoxybenzamine, but not of beta-receptors by propranolol, also reduced the duration of the convulsion. The presynaptic alpha-agonist, clonidine, at either 1 mg/kg or 0.05 mg/kg also reduced the magnitude of the convulsion but either blockade of dopamine receptors with pimozide or destruction of the ascending dopamine systems by 6-hydroxydopamine was without effect. It is concluded that dopamine systems are not involved in post-decapitation convulsions and that the noradrenergic involvement is by the descending spinal projections acting on a post-synaptic alpha-receptor in the spinal cord, but also modulated by presynaptic alpha-receptors possibly on the locus coeruleus perikarya.", "contents": "Physiological function of descending noradrenaline projections to the spinal cord: role in post-decapitation convulsions. Destruction of the descending noradrenergic innervation to the spinal cord, but not that to the cerebellum or the forebrain, by the use of intracerebral injection of 6-hydroxydopamine completely prevented the occurrence of the usual itation convulsion. Depletion of brain noradrenaline by synthesis inhibition with DDC, FLA 57 or FLA 63 g reduced the duration of the post-decapitation convulsion. Blockade of alpha-noradrenergic receptors by phentolamine or phenoxybenzamine, but not of beta-receptors by propranolol, also reduced the duration of the convulsion. The presynaptic alpha-agonist, clonidine, at either 1 mg/kg or 0.05 mg/kg also reduced the magnitude of the convulsion but either blockade of dopamine receptors with pimozide or destruction of the ascending dopamine systems by 6-hydroxydopamine was without effect. It is concluded that dopamine systems are not involved in post-decapitation convulsions and that the noradrenergic involvement is by the descending spinal projections acting on a post-synaptic alpha-receptor in the spinal cord, but also modulated by presynaptic alpha-receptors possibly on the locus coeruleus perikarya."}
{"id": "PMID:477740", "title": "GABA modulates the release of dopamine and acetylcholine from rat caudate nucleus slices.", "content": "The effects of GABA on depolarization-induced (26 mM K+) release of radiolabeled dopamine (DA) and acetylcholine (ACh) from slices of rat caudate nucleus were examined with a superfusion method. GABA, in concentrations of 10(-5)--10(-3) M, dose-dependently enhanced the release of DA, either accumulated by high-affinity uptake or synthesized from 14-C-tyrosine. In contrast, the release of ACh was reduced by GABA. This reduction appeared to be caused by the increase in DA-release. These effects of GABA decreased from the caudal to rostral part within the caudate nucleus, an order which parallels the distribution of endogenous GABA and glutamic acid decarboxylase. However, GABA had little, if any, effect in the nucleus accumbens. Since it was difficult to antagonize the effects of GABA on DA and ACh release with bicuculline or picrotoxin, it remains uncertain whether these effects were mediated via GABA receptors. In view of the high endogenous GABA level in the caudate nucleus it is concluded that GABA may be one of the local factors involved in the control of the amount of transmitter that will be released from dopaminergic varicosities upon depolarization.", "contents": "GABA modulates the release of dopamine and acetylcholine from rat caudate nucleus slices. The effects of GABA on depolarization-induced (26 mM K+) release of radiolabeled dopamine (DA) and acetylcholine (ACh) from slices of rat caudate nucleus were examined with a superfusion method. GABA, in concentrations of 10(-5)--10(-3) M, dose-dependently enhanced the release of DA, either accumulated by high-affinity uptake or synthesized from 14-C-tyrosine. In contrast, the release of ACh was reduced by GABA. This reduction appeared to be caused by the increase in DA-release. These effects of GABA decreased from the caudal to rostral part within the caudate nucleus, an order which parallels the distribution of endogenous GABA and glutamic acid decarboxylase. However, GABA had little, if any, effect in the nucleus accumbens. Since it was difficult to antagonize the effects of GABA on DA and ACh release with bicuculline or picrotoxin, it remains uncertain whether these effects were mediated via GABA receptors. In view of the high endogenous GABA level in the caudate nucleus it is concluded that GABA may be one of the local factors involved in the control of the amount of transmitter that will be released from dopaminergic varicosities upon depolarization."}
{"id": "PMID:477741", "title": "Analgesia produced by microinjection of baclofen and morphine at brain stem sites.", "content": "Microinjection of either baclofen (1.5 microgram) or morphine (2.5 microgram), in equimolar doses (14 mM), at sites located in the caudal periaqueductal gray (PAG) resulted in a delay in tail flick latency (analgesia). The relative analgesic potency of baclofen among caudal PAG sites, however, did not correlate with that of morphine. Application of either drug into the caudal aspect of the cerebral aqueduct also produced analgesia, but neither agent caused analgesia when applied at PAG sites rostral to the interaural line. Baclofen also produced analgesia when microinjected in the lower brain stem at sites lateral to the midline in or near the nucleus gigantocellularis, but did not produce analgesia when applied on the midline at sites located within or near the raphe magnus. Conversely, morphine produced analgesia when applied locally at midline sites but not at sites located lateral to the midline. These data suggest that the analgesia produced by systemic administration of baclofen and morphine involves activation of different neuronal substrates.", "contents": "Analgesia produced by microinjection of baclofen and morphine at brain stem sites. Microinjection of either baclofen (1.5 microgram) or morphine (2.5 microgram), in equimolar doses (14 mM), at sites located in the caudal periaqueductal gray (PAG) resulted in a delay in tail flick latency (analgesia). The relative analgesic potency of baclofen among caudal PAG sites, however, did not correlate with that of morphine. Application of either drug into the caudal aspect of the cerebral aqueduct also produced analgesia, but neither agent caused analgesia when applied at PAG sites rostral to the interaural line. Baclofen also produced analgesia when microinjected in the lower brain stem at sites lateral to the midline in or near the nucleus gigantocellularis, but did not produce analgesia when applied on the midline at sites located within or near the raphe magnus. Conversely, morphine produced analgesia when applied locally at midline sites but not at sites located lateral to the midline. These data suggest that the analgesia produced by systemic administration of baclofen and morphine involves activation of different neuronal substrates."}
{"id": "PMID:477742", "title": "Action of indapamide on excitation-contraction coupling in vascular smooth muscle.", "content": "The effects of indapamide on electrophysiological and mechanical parameters of longitudinal smooth muscle strips isolated from mammalian portal vein were studied by means of a double sucrose gap method associated with a photoelectric device for recording contraction. Indapamide (10(-4) M) reduced both the amplitude of the action potential and the contraction. The calcium inward current decreased and consequently the phasic contraction was also reduced. The potassium outward current was diminished while the tonic contraction was not modified significantly. The depressant nature of the indapamide response could counterbalance the stimulating action of angiotensin II but not that of noradrenaline. The results suggest  that indapamide acts primarily on the plasma membrane of vascular smooth muscle by reducing the transmembrane calcium current, although a secondary decrease in the intracellular bound calcium could not be completely excluded.", "contents": "Action of indapamide on excitation-contraction coupling in vascular smooth muscle. The effects of indapamide on electrophysiological and mechanical parameters of longitudinal smooth muscle strips isolated from mammalian portal vein were studied by means of a double sucrose gap method associated with a photoelectric device for recording contraction. Indapamide (10(-4) M) reduced both the amplitude of the action potential and the contraction. The calcium inward current decreased and consequently the phasic contraction was also reduced. The potassium outward current was diminished while the tonic contraction was not modified significantly. The depressant nature of the indapamide response could counterbalance the stimulating action of angiotensin II but not that of noradrenaline. The results suggest  that indapamide acts primarily on the plasma membrane of vascular smooth muscle by reducing the transmembrane calcium current, although a secondary decrease in the intracellular bound calcium could not be completely excluded."}
{"id": "PMID:477743", "title": "Effects of alkyl analogs of histamine and metiamide on gastric acid secretion.", "content": "Histamine and metiamide analogs with alkyl, methyl, ethyl or isopropyl groups on the imidazole ring in position 5, not previously tested on an in vivo preparation, were studied for their effects on gastric acid secretion in cats with gastric fistulas. Our findings confirmed that the methyl group appears to be optimal for an effect on gastric acid secretion as regards both agonism and antagonism. Ethyl substitution apparently did not change the pharmacological activity of histamine but modified the inhibitory effect of metiamide from competitive to non-competitive kinetics. Isopropyl analogs were inactive on gastric secretion under our experimental conditions.", "contents": "Effects of alkyl analogs of histamine and metiamide on gastric acid secretion. Histamine and metiamide analogs with alkyl, methyl, ethyl or isopropyl groups on the imidazole ring in position 5, not previously tested on an in vivo preparation, were studied for their effects on gastric acid secretion in cats with gastric fistulas. Our findings confirmed that the methyl group appears to be optimal for an effect on gastric acid secretion as regards both agonism and antagonism. Ethyl substitution apparently did not change the pharmacological activity of histamine but modified the inhibitory effect of metiamide from competitive to non-competitive kinetics. Isopropyl analogs were inactive on gastric secretion under our experimental conditions."}
{"id": "PMID:477744", "title": "Genetic variability within and between outbred Wistar strains of rats.", "content": "The allelic frequencies at 6 isozyme loci (Es-1, Es-2, Es-3, Es-4, Es-Si and Amy-1) were examined in 4 outbred Wistar strains and additionally 2 SD strains as controls. From the allelic frequencies, the averages of the heterozygosities in individual strains and the geometric genetic distances between every pair of all possible strain combinations were calculated. The averages of the heterozygosities in 2 SD strains were both intermediate (around 0.2) and the genetic distance between them was rather short. But among the Wistar strains, the averages of the heterozygosities were highly variable and the genetic relationships among them were very variable in their genetic distances. From these results, it was suggested that the outbred Wistar strains were different each other in their genetic constitutions and that no suggestion was obtained to descriminate genetically the Wistar strains from the SD strains.", "contents": "Genetic variability within and between outbred Wistar strains of rats. The allelic frequencies at 6 isozyme loci (Es-1, Es-2, Es-3, Es-4, Es-Si and Amy-1) were examined in 4 outbred Wistar strains and additionally 2 SD strains as controls. From the allelic frequencies, the averages of the heterozygosities in individual strains and the geometric genetic distances between every pair of all possible strain combinations were calculated. The averages of the heterozygosities in 2 SD strains were both intermediate (around 0.2) and the genetic distance between them was rather short. But among the Wistar strains, the averages of the heterozygosities were highly variable and the genetic relationships among them were very variable in their genetic distances. From these results, it was suggested that the outbred Wistar strains were different each other in their genetic constitutions and that no suggestion was obtained to descriminate genetically the Wistar strains from the SD strains."}
{"id": "PMID:477745", "title": "[Comparison of different types automatic water-supply system for mouse rearing (author's transl)].", "content": "Rearing and breeding scores were compared between groups of mice (JCL : ICR and ddN strains) raised with two different types of automatic water-supply systems; the Japanese type and the American type, using manual water-supply system as control. The mice raised with the manual water-supply system were superior in body weight gain as compared to those with two automatic water-supply systems. As to the survival rate, however, the m;  anual water-supply system and the Japanese type gave better results than the American type. As to weanling rate in the breeding test, the manual water-supply system gave somewhat better result than either of the two automatic types. Accidental water leaks, which are serious problems of automatic systems, occurred frequently only when the American type was used. Only one defect of the Japanese type revealed was that it was unfavorable for mice with smaller size (e.g., young ddN mice), resulting in lower body weight gain as well as lower breeding scores.", "contents": "[Comparison of different types automatic water-supply system for mouse rearing (author's transl)]. Rearing and breeding scores were compared between groups of mice (JCL : ICR and ddN strains) raised with two different types of automatic water-supply systems; the Japanese type and the American type, using manual water-supply system as control. The mice raised with the manual water-supply system were superior in body weight gain as compared to those with two automatic water-supply systems. As to the survival rate, however, the m;  anual water-supply system and the Japanese type gave better results than the American type. As to weanling rate in the breeding test, the manual water-supply system gave somewhat better result than either of the two automatic types. Accidental water leaks, which are serious problems of automatic systems, occurred frequently only when the American type was used. Only one defect of the Japanese type revealed was that it was unfavorable for mice with smaller size (e.g., young ddN mice), resulting in lower body weight gain as well as lower breeding scores."}
{"id": "PMID:477746", "title": "[The incidences of spontaneous fetal anomalies in Japanese white rabbits (author's transl)].", "content": "The incidences of spontaneous fetal anomalies in coccidium-free Japanese white rabbits bred and kept in our laboratories are reported. Live fetuses were removed from 87 (95.6%) out of 91 maternal animals examined, totaling 538 (295 males and 243 females). of them, 9 fetuses (1.7%) showed external and visceral abnormalities such as hydrocephalia (5 cases), short tail (2), anophthalmia (1) and talipomanus flexa (1). Skeletal abnormalities were found in 7 (1.7%) of 424 fetues examined.", "contents": "[The incidences of spontaneous fetal anomalies in Japanese white rabbits (author's transl)]. The incidences of spontaneous fetal anomalies in coccidium-free Japanese white rabbits bred and kept in our laboratories are reported. Live fetuses were removed from 87 (95.6%) out of 91 maternal animals examined, totaling 538 (295 males and 243 females). of them, 9 fetuses (1.7%) showed external and visceral abnormalities such as hydrocephalia (5 cases), short tail (2), anophthalmia (1) and talipomanus flexa (1). Skeletal abnormalities were found in 7 (1.7%) of 424 fetues examined."}
{"id": "PMID:477747", "title": "[A survey of Brucella canis infection in dogs sheltered in Tohoku University School of Medicine (author's transl)].", "content": "A total number of 1549 dogs from Miyagi Prefecture and the north coast area of Fukushima Prfecture were surveyed during a year from December 1976 to November 1977 for Brucella canis (B. canis). 173 of 1549 dogs (11.2%) were sero-positive to B. canis, and B. canis was isolated from 55 of 148 dogs examined, giving more than 3.6% for total 1549 dogs. The positivity were higher than that have been reported from other areas. The results of the morphological and biochemical studies for isolates were similar to the reference strain RM6/66, and they were identified as B. canis. No significant difference in sero-positive rate was found between male and female, but isolation rate was higher in female than in male. B. canis was isolated most frequently from the uterus and the spleen of both sexes. The importance of urine and milk as a source of infection was discussed as well as the role of infected focus at non gravid uterus for abortion and fetal infection. A case supposed to be a vertical infection was described also.", "contents": "[A survey of Brucella canis infection in dogs sheltered in Tohoku University School of Medicine (author's transl)]. A total number of 1549 dogs from Miyagi Prefecture and the north coast area of Fukushima Prfecture were surveyed during a year from December 1976 to November 1977 for Brucella canis (B. canis). 173 of 1549 dogs (11.2%) were sero-positive to B. canis, and B. canis was isolated from 55 of 148 dogs examined, giving more than 3.6% for total 1549 dogs. The positivity were higher than that have been reported from other areas. The results of the morphological and biochemical studies for isolates were similar to the reference strain RM6/66, and they were identified as B. canis. No significant difference in sero-positive rate was found between male and female, but isolation rate was higher in female than in male. B. canis was isolated most frequently from the uterus and the spleen of both sexes. The importance of urine and milk as a source of infection was discussed as well as the role of infected focus at non gravid uterus for abortion and fetal infection. A case supposed to be a vertical infection was described also."}
{"id": "PMID:477749", "title": "[Sturctural outline of spontaneous motor activity monitor using infrared photoelectric switch for small animals (author's transl)].", "content": "A simple monitor was developed for measuring the spontaneous motor activity (SMA) of small laboratory animals. The monitor was designed to measure animal ambulation along a horizontal axis by means of two infrared photo-beams. Using the monitor, male mice 7 to weeks of age at the commencement of this experiemtn showed high SMA values for the first 15 min and low values for the last 15 min of the experiment.", "contents": "[Sturctural outline of spontaneous motor activity monitor using infrared photoelectric switch for small animals (author's transl)]. A simple monitor was developed for measuring the spontaneous motor activity (SMA) of small laboratory animals. The monitor was designed to measure animal ambulation along a horizontal axis by means of two infrared photo-beams. Using the monitor, male mice 7 to weeks of age at the commencement of this experiemtn showed high SMA values for the first 15 min and low values for the last 15 min of the experiment."}
{"id": "PMID:477767", "title": "Quantitative development of adherent cell colonies in bone marrow cell culture in vitro.", "content": "Quantification of the formation of adherent cell colonies in bone marrow cell culture was attempted. By secondary transfer of the bone marrow cells as a single cell suspension after 4 days' culture of fine marrow fragments, a linear relationship was obtained between the number of adherent cell colonies developing and the number of cells secondarily inoculated into the culture bottle. This suggests that 4 days' culture of the bone marrow cells with close intercellular interactions is sufficient for the 'conditioning' of the cells to develop adherent cell colonies. Activity of such colonies to support haemopoietic stem cell proliferation was also shown.", "contents": "Quantitative development of adherent cell colonies in bone marrow cell culture in vitro. Quantification of the formation of adherent cell colonies in bone marrow cell culture was attempted. By secondary transfer of the bone marrow cells as a single cell suspension after 4 days' culture of fine marrow fragments, a linear relationship was obtained between the number of adherent cell colonies developing and the number of cells secondarily inoculated into the culture bottle. This suggests that 4 days' culture of the bone marrow cells with close intercellular interactions is sufficient for the 'conditioning' of the cells to develop adherent cell colonies. Activity of such colonies to support haemopoietic stem cell proliferation was also shown."}
{"id": "PMID:477768", "title": "The role of hemin in the regulation of heme synthesis by fetal mouse liver erythroblasts in culture.", "content": "The regulatory role of exogenous hemin on the heme synthetic pathway was studied in fetal mouse liver erythroblasts in culture. Hemin added to culture medium of 13th day embryo liver cells inhibited, dose dependently, the incorporation of the porphyrin precursors, 59Fe, 14C-2-glycine and 14C-5-aminolevulinic acid (ALA) by 85%, 70% and 45%, respectively. This suggests a multiple effect of hemin on the porphyrin biosynthetic enzymes. Exogenous ALA competed with 14 C-2-glycine as a porphyrin precursor, but the rate of heme synthesis, measured by 59Fe incorporation, remained unaltered. Protoporphyrin mimicked the hemin effect on the inhibition of glycine incorporation into heme, but reduced iron incorporation by only 20%. Erythroblasts, with an inhibited porphyrin biosynthesis, utilized exogenous 59Fe-hemin for hemoglobin assembly and maintained an undecreased level of hemoglobin synthesis. The results indicate that hemin inhibits the porphyrin biosynthesis in fetal mouse liver erythroblasts mainly at the iron incorporation stage.", "contents": "The role of hemin in the regulation of heme synthesis by fetal mouse liver erythroblasts in culture. The regulatory role of exogenous hemin on the heme synthetic pathway was studied in fetal mouse liver erythroblasts in culture. Hemin added to culture medium of 13th day embryo liver cells inhibited, dose dependently, the incorporation of the porphyrin precursors, 59Fe, 14C-2-glycine and 14C-5-aminolevulinic acid (ALA) by 85%, 70% and 45%, respectively. This suggests a multiple effect of hemin on the porphyrin biosynthetic enzymes. Exogenous ALA competed with 14 C-2-glycine as a porphyrin precursor, but the rate of heme synthesis, measured by 59Fe incorporation, remained unaltered. Protoporphyrin mimicked the hemin effect on the inhibition of glycine incorporation into heme, but reduced iron incorporation by only 20%. Erythroblasts, with an inhibited porphyrin biosynthesis, utilized exogenous 59Fe-hemin for hemoglobin assembly and maintained an undecreased level of hemoglobin synthesis. The results indicate that hemin inhibits the porphyrin biosynthesis in fetal mouse liver erythroblasts mainly at the iron incorporation stage."}
{"id": "PMID:477770", "title": "The role of fibroblastoid cells and macrophages from mouse bone marrow in the in vitro growth promotion of haemopoietic tumour cells.", "content": "Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. The latter were characterized by means of cell density distribution analysis. They had a broad distribution pattern and an average peak cell density of 1.069 g.cm-3. The growth promotion activity exerted by adherent cell layers from the various density fractions on the AVRij-1 tumour cell line coincided with the distribution of fibroblastoid colony forming units. On the other hand, the presence of macrophages in the adherent layers seemed to be non-essential for the in vitro promotion of growth of the AVRij-1 cell line.", "contents": "The role of fibroblastoid cells and macrophages from mouse bone marrow in the in vitro growth promotion of haemopoietic tumour cells. Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. The latter were characterized by means of cell density distribution analysis. They had a broad distribution pattern and an average peak cell density of 1.069 g.cm-3. The growth promotion activity exerted by adherent cell layers from the various density fractions on the AVRij-1 tumour cell line coincided with the distribution of fibroblastoid colony forming units. On the other hand, the presence of macrophages in the adherent layers seemed to be non-essential for the in vitro promotion of growth of the AVRij-1 cell line."}
{"id": "PMID:477771", "title": "Erythropoiesis in vitro. III. The role of potassium ions in erythroid colony formation.", "content": "The role of potassium as an essential promotor of erythroid progenitor growth (BFU-e & CFU-e) from normal murine hematopoietic tissues was studied. Dialyzed fetal calf serum, over a wide range of concentrations, was shown to reduce the numbers of BFU-e and CFU-e that could be cultured from normal murine bone marrow. A dose-dependent addition of 1 M KC1 restored erythroid progenitor colony growth to the levels generally seen when normal, non-dialyzed fetal calf serum was used. Furthermore, when [K] was increased in some human urinary and sheep plasma erythropoietin preparations, the number of erythroid progenitor cells cultured also increased. This influence is crucial to the differentiation of committed stem cells into the erythroid pathway and must therefore be considered in the development of serum-free growth media.", "contents": "Erythropoiesis in vitro. III. The role of potassium ions in erythroid colony formation. The role of potassium as an essential promotor of erythroid progenitor growth (BFU-e & CFU-e) from normal murine hematopoietic tissues was studied. Dialyzed fetal calf serum, over a wide range of concentrations, was shown to reduce the numbers of BFU-e and CFU-e that could be cultured from normal murine bone marrow. A dose-dependent addition of 1 M KC1 restored erythroid progenitor colony growth to the levels generally seen when normal, non-dialyzed fetal calf serum was used. Furthermore, when [K] was increased in some human urinary and sheep plasma erythropoietin preparations, the number of erythroid progenitor cells cultured also increased. This influence is crucial to the differentiation of committed stem cells into the erythroid pathway and must therefore be considered in the development of serum-free growth media."}
{"id": "PMID:477772", "title": "Hemopoiesis of the cultured whole mouse embryo.", "content": "In vitro cultured murine embryos provide a valuable methodology for studying the ontogeny of hemopoiesis in mammalian embryos. Under improved conditions, 49% of mouse embryos cultured from the blastocyst stage reached the early somite stage. The development of the embryo was arrested at different stages by omitting human cord serum from the culture medium at specific times. These observations suggest the appearance and differentiation of the first hemopoietic cells in the mouse yolk sac to be independent of a normal embryo. In the yolk sac of all cultured mouse embryos, hemopoietic cell differentiated into a major cell type, erythroblasts, and minor cell types such as monocytes, macrophages, and granulocytic cells. A small number of hemopoietic cells remained undifferentiated.", "contents": "Hemopoiesis of the cultured whole mouse embryo. In vitro cultured murine embryos provide a valuable methodology for studying the ontogeny of hemopoiesis in mammalian embryos. Under improved conditions, 49% of mouse embryos cultured from the blastocyst stage reached the early somite stage. The development of the embryo was arrested at different stages by omitting human cord serum from the culture medium at specific times. These observations suggest the appearance and differentiation of the first hemopoietic cells in the mouse yolk sac to be independent of a normal embryo. In the yolk sac of all cultured mouse embryos, hemopoietic cell differentiated into a major cell type, erythroblasts, and minor cell types such as monocytes, macrophages, and granulocytic cells. A small number of hemopoietic cells remained undifferentiated."}
{"id": "PMID:477773", "title": "Lithium carbonate increases marrow granulocyte-committed colony-forming units and peripheral blood granulocytes in a canine model.", "content": "Psychiatric patients given lithium carbonate (LC) commonly develop granulocytosis of the peripheral blood. This is an important observation with several potential applications in clinical hematology and oncology. In order to study further this phenomenon, we have developed an animal model of LC-induced granulocytosis. Four mongrel dogs weighing 23--28 kg were given commercially available LC in 300 mg capsules by mouth twice daily. Peripheral blood was examined twice weekly and marrow granylocyte-committed colony forming units (CFU-c) were studied weekly. Significant increments of peripheral granulocytes (P = 0.003) and of marrow CFU-c (P =0.001) were noted. An increased number of marrow CFU-c has not been previously reported but is consistent with other knowledge about this phenomenon. We conclude that this convenient animal model may be of value in further studies of LC-induced granulocytosis and its possible applications.", "contents": "Lithium carbonate increases marrow granulocyte-committed colony-forming units and peripheral blood granulocytes in a canine model. Psychiatric patients given lithium carbonate (LC) commonly develop granulocytosis of the peripheral blood. This is an important observation with several potential applications in clinical hematology and oncology. In order to study further this phenomenon, we have developed an animal model of LC-induced granulocytosis. Four mongrel dogs weighing 23--28 kg were given commercially available LC in 300 mg capsules by mouth twice daily. Peripheral blood was examined twice weekly and marrow granylocyte-committed colony forming units (CFU-c) were studied weekly. Significant increments of peripheral granulocytes (P = 0.003) and of marrow CFU-c (P =0.001) were noted. An increased number of marrow CFU-c has not been previously reported but is consistent with other knowledge about this phenomenon. We conclude that this convenient animal model may be of value in further studies of LC-induced granulocytosis and its possible applications."}
{"id": "PMID:477774", "title": "Acid phosphatase in normal human blood cells: characterization by isoelectric focusing in polyacrylamide gel.", "content": "Using the high resolution technique of isoelectric focusing in polyacrylamide gel, isoenzymatic components could be discerned in acid phosphatase extracted from normal human lymphocytes, granulocytes, monocytes, and platelets. For each cell type, distinctive isoenzymatic patterns of acid phosphatase activity could be observed. These studies provide further evidence that various types of cellular differentiation are associated with differences in isoenzymatic components of acid phosphatase.", "contents": "Acid phosphatase in normal human blood cells: characterization by isoelectric focusing in polyacrylamide gel. Using the high resolution technique of isoelectric focusing in polyacrylamide gel, isoenzymatic components could be discerned in acid phosphatase extracted from normal human lymphocytes, granulocytes, monocytes, and platelets. For each cell type, distinctive isoenzymatic patterns of acid phosphatase activity could be observed. These studies provide further evidence that various types of cellular differentiation are associated with differences in isoenzymatic components of acid phosphatase."}
{"id": "PMID:477775", "title": "Tangential orientation and spatial order in dendrites of cat auditory cortex: a computer microscope study of Golgi-impregnated material.", "content": "In the tangential plane (parallel to the pial surface) dendrites in the primary auditory cortex (A1) of cat were found to exhibit preferentially oriented growth. This was shown by means of a computer microscope study of Golgi-Cox stained neurons as seen in 100 micrometers and 300 micrometers thick tangential sections. Two techniques were used to represent the 3-dimensional structure of dendrites: the \"dendritic stick\" and the \"dendritic trumpet\". The former dismembers a dendrite into its individual segments; the latter considers a dendrite as an entity and represents it by its centroid, its moments and the spatial dispersionof its branches. Both statistical and Fourier analyses of the data show that within the tangential plane there is a significant and consistent orientation of the dendritic sticks in a dorso-ventral direction which seems correlated with the cortical isofrequency contours observed in electrophysiological maps of the A1 region. The dendritic trumpet analyses also show a distinctly non-random vertical distribution of pyramidal cell basal dendrites but not of stellate cell dendrites.", "contents": "Tangential orientation and spatial order in dendrites of cat auditory cortex: a computer microscope study of Golgi-impregnated material. In the tangential plane (parallel to the pial surface) dendrites in the primary auditory cortex (A1) of cat were found to exhibit preferentially oriented growth. This was shown by means of a computer microscope study of Golgi-Cox stained neurons as seen in 100 micrometers and 300 micrometers thick tangential sections. Two techniques were used to represent the 3-dimensional structure of dendrites: the \"dendritic stick\" and the \"dendritic trumpet\". The former dismembers a dendrite into its individual segments; the latter considers a dendrite as an entity and represents it by its centroid, its moments and the spatial dispersionof its branches. Both statistical and Fourier analyses of the data show that within the tangential plane there is a significant and consistent orientation of the dendritic sticks in a dorso-ventral direction which seems correlated with the cortical isofrequency contours observed in electrophysiological maps of the A1 region. The dendritic trumpet analyses also show a distinctly non-random vertical distribution of pyramidal cell basal dendrites but not of stellate cell dendrites."}
{"id": "PMID:477776", "title": "The projection of the visual cortex on the Clare-Bishop area in the cat. A degeneration study with the electron microscope.", "content": "Following large lesions of the cat visual cortex, the distribution of degenerating terminal boutons in the Clare-Bishop area was studied electron microscopically. Degenerating boutons were found throughout the cortical layers but mostly in layer III (51% of the total number of degenerating boutons) and layer V (24%). A smaller number of boutons were found in layers II (12%) and IV (9%), and very few in layers VI (3%) and I (1%). No degenerating terminals were observed in the upper two-thirds of layer I. Seventy-six per cent of the total degenerating boutons terminated on dendritic spines, 22% on dendritic shafts, and 2% on somata. Some degenerating boutons made synaptic contacts with somata and dendrites of nonpyramidal neurons. For example, one degenerating bouton was observed in contact with an apical dendrite of a fusiform cell. Three examples of dendritic spines, with which degenerating boutons made synaptic contracts, were found to belong to spinous stallate cells. No degenerating boutons were observed making synaptic contacts with profiles that could conclusively be traced to pyramidal cell somata.", "contents": "The projection of the visual cortex on the Clare-Bishop area in the cat. A degeneration study with the electron microscope. Following large lesions of the cat visual cortex, the distribution of degenerating terminal boutons in the Clare-Bishop area was studied electron microscopically. Degenerating boutons were found throughout the cortical layers but mostly in layer III (51% of the total number of degenerating boutons) and layer V (24%). A smaller number of boutons were found in layers II (12%) and IV (9%), and very few in layers VI (3%) and I (1%). No degenerating terminals were observed in the upper two-thirds of layer I. Seventy-six per cent of the total degenerating boutons terminated on dendritic spines, 22% on dendritic shafts, and 2% on somata. Some degenerating boutons made synaptic contacts with somata and dendrites of nonpyramidal neurons. For example, one degenerating bouton was observed in contact with an apical dendrite of a fusiform cell. Three examples of dendritic spines, with which degenerating boutons made synaptic contracts, were found to belong to spinous stallate cells. No degenerating boutons were observed making synaptic contacts with profiles that could conclusively be traced to pyramidal cell somata."}
{"id": "PMID:477777", "title": "Organization of rapid responses to postural and locomotor-like perturbations of standing man.", "content": "This study has described the organization of EMG activities among the muscles of a standing subject's legs during rapid postural adjustments (95--120 ms latencies). Adjustments were elicited by the horizontal translation of both feet (causing antero-posterior sway), by the synchronous vertical displacement of both feet (causing changes in height) and by the reciprocal vertical displacement of the feet (causing changes in height) and by the reciprocal vertical displacement of the feet (causing a locomotor-like motion of the legs and lateral sway of the body). The resulting patterns of EMG activity were highly specific for each kind of displacement, and all subjects completely reorganized the pattern of activity from one form to another within the first trials, even immediately following unexpected stimulus changes. The organization of EMG activities during reciprocal vertical displacements was qualitatively quite similar to those observed during the comparable swing and stance phases of the locomotor step cycle; flexor muscles of the ankle and knee (those being shortened by the displacement) contracted in the upwardly displaced leg while extensor muscles were active in the downwardly displaced leg. This pattern was in marked contrast to the activation of lengthening muscles during synchronous vertical and antero-posterior sway displacements. Finally, electrical cutaneous stimulation of the dorsum of one foot during reciprocal vertical displacements always enhanced the EMG activity of the agonist leg muscles, in-phase with the vertical movement.", "contents": "Organization of rapid responses to postural and locomotor-like perturbations of standing man. This study has described the organization of EMG activities among the muscles of a standing subject's legs during rapid postural adjustments (95--120 ms latencies). Adjustments were elicited by the horizontal translation of both feet (causing antero-posterior sway), by the synchronous vertical displacement of both feet (causing changes in height) and by the reciprocal vertical displacement of the feet (causing changes in height) and by the reciprocal vertical displacement of the feet (causing a locomotor-like motion of the legs and lateral sway of the body). The resulting patterns of EMG activity were highly specific for each kind of displacement, and all subjects completely reorganized the pattern of activity from one form to another within the first trials, even immediately following unexpected stimulus changes. The organization of EMG activities during reciprocal vertical displacements was qualitatively quite similar to those observed during the comparable swing and stance phases of the locomotor step cycle; flexor muscles of the ankle and knee (those being shortened by the displacement) contracted in the upwardly displaced leg while extensor muscles were active in the downwardly displaced leg. This pattern was in marked contrast to the activation of lengthening muscles during synchronous vertical and antero-posterior sway displacements. Finally, electrical cutaneous stimulation of the dorsum of one foot during reciprocal vertical displacements always enhanced the EMG activity of the agonist leg muscles, in-phase with the vertical movement."}
{"id": "PMID:477778", "title": "Organization of the convergence in the intermediate cerebellar nuclei of somatosensory receptive fields with motor cortical-evoked responses.", "content": "The convergence in the intermediate cerebellar nuclei of somatosensory inputs with those from the motor cortex was investigated in cats (1) by examining with natural stimulation the receptive field of nuclear neurons, and (2) by determining the response of the same neurons to stimulation of motor cortical sites which had been identified by microstimulation-evoked movements. Of 196 neurons studied, a response to natural stimulation could be identified in 143, more than half of which (83) were driven by passive movement of one or two joints. The predominant response to cortical microstimulation was a suppression of discharge, although the suppression was sometimes preceded by a weak facilitation or interrupted by a brief but strong facilitation. A pattern of convergence was identified in which 72% of those cells driven by passive joint movement in one direction were suppressed by stimulation of the cortical site from which movement could be evoked in the opposite direction. A model based on this convergence is proposed.", "contents": "Organization of the convergence in the intermediate cerebellar nuclei of somatosensory receptive fields with motor cortical-evoked responses. The convergence in the intermediate cerebellar nuclei of somatosensory inputs with those from the motor cortex was investigated in cats (1) by examining with natural stimulation the receptive field of nuclear neurons, and (2) by determining the response of the same neurons to stimulation of motor cortical sites which had been identified by microstimulation-evoked movements. Of 196 neurons studied, a response to natural stimulation could be identified in 143, more than half of which (83) were driven by passive movement of one or two joints. The predominant response to cortical microstimulation was a suppression of discharge, although the suppression was sometimes preceded by a weak facilitation or interrupted by a brief but strong facilitation. A pattern of convergence was identified in which 72% of those cells driven by passive joint movement in one direction were suppressed by stimulation of the cortical site from which movement could be evoked in the opposite direction. A model based on this convergence is proposed."}
{"id": "PMID:477779", "title": "Optic nerve fibre lesions in adult cats: pattern of recovery of spatial vision.", "content": "Partial deafferentation in the pre-geniculate optic pathways of the adult cat was produced by micro-injection of diphtheria toxin. In this lesion a certain proportion of fibres was damaged while neighbouring fibres remained unaffected. Behavioural methods were used to study the time course, pattern, and extent of recovery of spatial vision after such lesions. Cats were trained to discriminate square-wave gratings from blank fields. A range of grating sizes (0.13--2.61 cycles/degree) at high contrast were presented each day and when performance was consistently 100%, the lesion was placed. At 24 h post-lesion and serially thereafter grating discrimination was tested. Contrast sensitivity was also measured before and after the lesion. All cats recovered to pre-lesion performance levels. The time course of recovery was characterized by an early rapid phase followed by a longer slower phase. The early recovery was temporally coincident with dispersal of oedema at the site of the lesion, while the later slower recovery probably represented a functional reorganization at the synapse. Optic nerve fibre analyses at one year post-lesion showed reduction in fibre content ranging from 33 to 77% in different cats without shift in the fibre size histograms. The length of recovery time was directly related to the magnitude of fibre loss. The return of spatial frequency perception was hierarchical - first medium (1--4 days), then low (1--2 months) and finally high spatial frequencies (5--8 months). Possible mechanisms are discussed and it is suggested that such a pattern of psychophysical results could be accounted for by date than the X-type.", "contents": "Optic nerve fibre lesions in adult cats: pattern of recovery of spatial vision. Partial deafferentation in the pre-geniculate optic pathways of the adult cat was produced by micro-injection of diphtheria toxin. In this lesion a certain proportion of fibres was damaged while neighbouring fibres remained unaffected. Behavioural methods were used to study the time course, pattern, and extent of recovery of spatial vision after such lesions. Cats were trained to discriminate square-wave gratings from blank fields. A range of grating sizes (0.13--2.61 cycles/degree) at high contrast were presented each day and when performance was consistently 100%, the lesion was placed. At 24 h post-lesion and serially thereafter grating discrimination was tested. Contrast sensitivity was also measured before and after the lesion. All cats recovered to pre-lesion performance levels. The time course of recovery was characterized by an early rapid phase followed by a longer slower phase. The early recovery was temporally coincident with dispersal of oedema at the site of the lesion, while the later slower recovery probably represented a functional reorganization at the synapse. Optic nerve fibre analyses at one year post-lesion showed reduction in fibre content ranging from 33 to 77% in different cats without shift in the fibre size histograms. The length of recovery time was directly related to the magnitude of fibre loss. The return of spatial frequency perception was hierarchical - first medium (1--4 days), then low (1--2 months) and finally high spatial frequencies (5--8 months). Possible mechanisms are discussed and it is suggested that such a pattern of psychophysical results could be accounted for by date than the X-type."}
{"id": "PMID:477782", "title": "Dynamic role of vision in the control of posture in man.", "content": "The influence of moving visual surround on the manitenance of upright posture has been studied in man during combined motion of a platform (cart) on which subjects were standing. The normal visual surround was either moving together with the cart, or with a velocity equal to cart velocity either in the same or in the opposite direction of car motion (cart acceleration was either 0.2 m/s2 or 0.05 m/s2). The changes in body pitch observed under these three conditions of visual surround motion were in the same direction as those observed when visual surround motion was given in isolation. However, their amplitude was greater, particularly when visual surround motion was in conflict with body motion.", "contents": "Dynamic role of vision in the control of posture in man. The influence of moving visual surround on the manitenance of upright posture has been studied in man during combined motion of a platform (cart) on which subjects were standing. The normal visual surround was either moving together with the cart, or with a velocity equal to cart velocity either in the same or in the opposite direction of car motion (cart acceleration was either 0.2 m/s2 or 0.05 m/s2). The changes in body pitch observed under these three conditions of visual surround motion were in the same direction as those observed when visual surround motion was given in isolation. However, their amplitude was greater, particularly when visual surround motion was in conflict with body motion."}
{"id": "PMID:477781", "title": "Spatial properties of X and Y cells in the lateral geniculate nucleus of the cat and conduction veolcities of their inputs.", "content": "Visual neurons in the lateral geniculate nucleus (LGN) of the cat may be separated into distinct X and Y classes based on a test of the linearity of spatial summation. Y cells produce nonlinear responses especially when the visual stimulus is a fine spatial grating. X cells exhibit mainly linear summation properties. X cells respond mainly at the fundamental modulation frequency of a contrast reversal grating while Y cells respond at the fundamental and at the second harmonic of the modulation frequency. The spatial resolution of X cells' fundamental responses and Y cells' second harmonic responses is about the same, and both are two to eight times higher than the spatial resolution of the Y cells' fundamental response. The conduction velocity of the Y optic tract afferents is greater than that of the velocity of the X afferents. However, the LGN latencies of the responses of the two classes of cells to optic chiasm stimulation overlap considerably.", "contents": "Spatial properties of X and Y cells in the lateral geniculate nucleus of the cat and conduction veolcities of their inputs. Visual neurons in the lateral geniculate nucleus (LGN) of the cat may be separated into distinct X and Y classes based on a test of the linearity of spatial summation. Y cells produce nonlinear responses especially when the visual stimulus is a fine spatial grating. X cells exhibit mainly linear summation properties. X cells respond mainly at the fundamental modulation frequency of a contrast reversal grating while Y cells respond at the fundamental and at the second harmonic of the modulation frequency. The spatial resolution of X cells' fundamental responses and Y cells' second harmonic responses is about the same, and both are two to eight times higher than the spatial resolution of the Y cells' fundamental response. The conduction velocity of the Y optic tract afferents is greater than that of the velocity of the X afferents. However, the LGN latencies of the responses of the two classes of cells to optic chiasm stimulation overlap considerably."}
{"id": "PMID:477783", "title": "Some neurochemical studies on auditory regions of mouse brain.", "content": "The mouse brain auditory pathway has been dissected into five regions: geniculate bodies, posterior colliculi, superior olives, cochlear nuclei, and cochleas. The following analyses were made in these regions and in the auditory cortex: protein, glutamate, gamma-aminobutyrate, taurine, choline acetyltransferase, and glutamate decarboxylase. Taurine levels (nmol . mg of protein-) were highest in cortex (93) and geniculate bodies (60) and lowest in the cochlear nuclei (27) and cochleas (29). Concentrations of gamma-aminobutyrate (same units) were highest in the geniculate bodies (28), low in the superior olives and cochlear nuclei (9 to 10), and undetectable in the cochleas. The distribution of glutamate decarboxylase activity reflected that of gamma-aminobutyrate. The activities of choline acetyltransferase (nmol . of acetylcholine synthesized . h . -1 mg of protein-1) were highest in the superior olives (60) and low in the cochleas (3). These results are interpreted as biocyemical support for previous physiological and pharmacological identification of the olivo-cochlear bundle as cholinergic and the cochlear-nucleus neurones as non-cholinergic. The results also provide further evidence for a role of GABA in the posterior colliculi, but not in the cochleas.", "contents": "Some neurochemical studies on auditory regions of mouse brain. The mouse brain auditory pathway has been dissected into five regions: geniculate bodies, posterior colliculi, superior olives, cochlear nuclei, and cochleas. The following analyses were made in these regions and in the auditory cortex: protein, glutamate, gamma-aminobutyrate, taurine, choline acetyltransferase, and glutamate decarboxylase. Taurine levels (nmol . mg of protein-) were highest in cortex (93) and geniculate bodies (60) and lowest in the cochlear nuclei (27) and cochleas (29). Concentrations of gamma-aminobutyrate (same units) were highest in the geniculate bodies (28), low in the superior olives and cochlear nuclei (9 to 10), and undetectable in the cochleas. The distribution of glutamate decarboxylase activity reflected that of gamma-aminobutyrate. The activities of choline acetyltransferase (nmol . of acetylcholine synthesized . h . -1 mg of protein-1) were highest in the superior olives (60) and low in the cochleas (3). These results are interpreted as biocyemical support for previous physiological and pharmacological identification of the olivo-cochlear bundle as cholinergic and the cochlear-nucleus neurones as non-cholinergic. The results also provide further evidence for a role of GABA in the posterior colliculi, but not in the cochleas."}
{"id": "PMID:477784", "title": "Cooperative neural processes involved in stereoscopic acuity.", "content": "Results of psychophysical experiments are reported showing that synchrony, appropriate relative placement, and absence of standing disparity are important conditions to be met by members of a target configuration if they are to participate in the cooperative neural processes leading to the best disparity discrimination. Consecutive binocular presentation of the members of a stereo target decreases stereoacuity by a factor of about 10, and a step disparity displacement of a single line target needs to be larger still to be detected as a depth stimulus. A standing disaprity of even one minute of arc at least doubles the disaprity disxrimination threshold. It is postulated that a differencing mechanism operates on the depth signal of individual features; the temporal and spatial optima of target presentation for stereoscopic acuity outline the character of the concerned operations.", "contents": "Cooperative neural processes involved in stereoscopic acuity. Results of psychophysical experiments are reported showing that synchrony, appropriate relative placement, and absence of standing disparity are important conditions to be met by members of a target configuration if they are to participate in the cooperative neural processes leading to the best disparity discrimination. Consecutive binocular presentation of the members of a stereo target decreases stereoacuity by a factor of about 10, and a step disparity displacement of a single line target needs to be larger still to be detected as a depth stimulus. A standing disaprity of even one minute of arc at least doubles the disaprity disxrimination threshold. It is postulated that a differencing mechanism operates on the depth signal of individual features; the temporal and spatial optima of target presentation for stereoscopic acuity outline the character of the concerned operations."}
{"id": "PMID:477821", "title": "On the lipid granularity of renomedullary interstitial cells in benign and malignant courses of renal hypertension.", "content": "The number of lipid droplets of the renal medullary interstitial cells was registered in 40 rats with \"two-kidney Goldblatt hypertension\" and in 27 sham operated normotensive controls. A strong degranulation in the unclaimed kidneys was always associated with the malignant course of hypertension, characterized by a disturbance in the sodium and water balance. Giving 2 per cent saline in addition to demineralized water as a drinking fluid the decrease in the number of granules was prevented in most of the malignant cases. Significant changes in the granule count were never registered at a benign course of hypertension. Degranulation of the medullary interstitial cells in the unclamped kidneys does not unequivocally represent the loss of medullary antihypertensive function. It seems to be directly determined by the disturbance of sodium and water balance and indirectly by the hypertension.", "contents": "On the lipid granularity of renomedullary interstitial cells in benign and malignant courses of renal hypertension. The number of lipid droplets of the renal medullary interstitial cells was registered in 40 rats with \"two-kidney Goldblatt hypertension\" and in 27 sham operated normotensive controls. A strong degranulation in the unclaimed kidneys was always associated with the malignant course of hypertension, characterized by a disturbance in the sodium and water balance. Giving 2 per cent saline in addition to demineralized water as a drinking fluid the decrease in the number of granules was prevented in most of the malignant cases. Significant changes in the granule count were never registered at a benign course of hypertension. Degranulation of the medullary interstitial cells in the unclamped kidneys does not unequivocally represent the loss of medullary antihypertensive function. It seems to be directly determined by the disturbance of sodium and water balance and indirectly by the hypertension."}
{"id": "PMID:477822", "title": "Inhibition of adrenalin depletion in hypoglycaemic rats following permanent adrenalin application by depot capsule.", "content": "Chronic treatment of rats with adrenalin by means of a subcutaneous depot capsule (cont. 40 mg dry adrenalin/HCl, Merck, ca. 10 mg ascorbic acid and ca. 0.4 ml 0.9% NaCl, and sealed with a dialysis membrane to provide constant adrenalin output) leads after a primary hyperglycaemic phase to hypoglycaemia. At the onset of hypoglycaemia the adrenalin contents of the suprarenal glands decrease to levels of about one third of their normal state. Simultaneous glucose infusions by intraperitoneal depot capsules prevent hypoglycaemia as well as adrenalin depletion. The fact that adrenalin depletion does not always correspond with blood sugar levels below 80 mg/dl and that this depletion could be prevented by glucose infusions altogether seems to provide a strong hint that the mentioned depletion is due to hypersecretion and not to the blocking of adrenalin synthesis. If is true, then there must be no direct negative feed-back system between adrenalin secretion and its extraglandular level.", "contents": "Inhibition of adrenalin depletion in hypoglycaemic rats following permanent adrenalin application by depot capsule. Chronic treatment of rats with adrenalin by means of a subcutaneous depot capsule (cont. 40 mg dry adrenalin/HCl, Merck, ca. 10 mg ascorbic acid and ca. 0.4 ml 0.9% NaCl, and sealed with a dialysis membrane to provide constant adrenalin output) leads after a primary hyperglycaemic phase to hypoglycaemia. At the onset of hypoglycaemia the adrenalin contents of the suprarenal glands decrease to levels of about one third of their normal state. Simultaneous glucose infusions by intraperitoneal depot capsules prevent hypoglycaemia as well as adrenalin depletion. The fact that adrenalin depletion does not always correspond with blood sugar levels below 80 mg/dl and that this depletion could be prevented by glucose infusions altogether seems to provide a strong hint that the mentioned depletion is due to hypersecretion and not to the blocking of adrenalin synthesis. If is true, then there must be no direct negative feed-back system between adrenalin secretion and its extraglandular level."}
{"id": "PMID:477823", "title": "Monosodium glutamate in the chick embryo: lack of obvious toxic or teratogenic effects when administered at 24 hours incubation.", "content": "The administration of monosodium glutamate (MSC) to chick embryos at 24 hours did neither produce gross developmental defects nor influence growth by 72 hours total incubation. The concentrations of MSG administered ranged from 0.3 to 46.6%, the volumes injected being 0.05, 0.1, or 0.2 ml. Several injection methods were evaluated. It is suggested that injection into the centre of the yolk is the most effective way to give water-soluble treatments during this period of development.", "contents": "Monosodium glutamate in the chick embryo: lack of obvious toxic or teratogenic effects when administered at 24 hours incubation. The administration of monosodium glutamate (MSC) to chick embryos at 24 hours did neither produce gross developmental defects nor influence growth by 72 hours total incubation. The concentrations of MSG administered ranged from 0.3 to 46.6%, the volumes injected being 0.05, 0.1, or 0.2 ml. Several injection methods were evaluated. It is suggested that injection into the centre of the yolk is the most effective way to give water-soluble treatments during this period of development."}
{"id": "PMID:477825", "title": "The effect of heparin on lysosomes of the dog pancreas during acute experimental pancreatitis.", "content": "In 12 dogs with acute experimental pancreatitis (AEP) and 6 control animals the \"free\", \"latent\" and \"total\" activity of acid phosphatase, beta-glucuronidase and cathepsins in whole homogenates of the pancreas, in a lysosomal-enriched subfraction and the supernatant of pancreatic tissue was estimated. AEP was induced by injection of bile salts and thrombin solution into the pancreatic duct. In 6 dogs the protection with heparin (1.5 mg/kg/body weight) immediately after producing AEP was applied. In AEP without any protection the free activity of hydrolases in the whole homogenate (80--90%) and in the lysosomal enriched subfraction (75--90%) was higher than in the controls (60--70% and 55--75% respectively), suggesting an augmented lysosomal fragility during the course of AEP. Heparin depressed the free activity of hydrolases to 60--80% in whole homogenates, and 64--75% in the lysosomal enriched subfraction. The release of cathepsins during incubation of the lysosomal-enriched subfraction in acidic medium was lower in the group with heparin treatment. The data obtained suggest the stabilising effect of heparin on the lysosomes of the pancreas during acute experimental pancreatitis in dogs.", "contents": "The effect of heparin on lysosomes of the dog pancreas during acute experimental pancreatitis. In 12 dogs with acute experimental pancreatitis (AEP) and 6 control animals the \"free\", \"latent\" and \"total\" activity of acid phosphatase, beta-glucuronidase and cathepsins in whole homogenates of the pancreas, in a lysosomal-enriched subfraction and the supernatant of pancreatic tissue was estimated. AEP was induced by injection of bile salts and thrombin solution into the pancreatic duct. In 6 dogs the protection with heparin (1.5 mg/kg/body weight) immediately after producing AEP was applied. In AEP without any protection the free activity of hydrolases in the whole homogenate (80--90%) and in the lysosomal enriched subfraction (75--90%) was higher than in the controls (60--70% and 55--75% respectively), suggesting an augmented lysosomal fragility during the course of AEP. Heparin depressed the free activity of hydrolases to 60--80% in whole homogenates, and 64--75% in the lysosomal enriched subfraction. The release of cathepsins during incubation of the lysosomal-enriched subfraction in acidic medium was lower in the group with heparin treatment. The data obtained suggest the stabilising effect of heparin on the lysosomes of the pancreas during acute experimental pancreatitis in dogs."}
{"id": "PMID:477826", "title": "Changes of cardiac and skeletal muscle in pigs following transport stress. An electron microscopic study.", "content": "After lorry transport (60--120 min), fattening pigs (100--120 kg living weight) with clinical signs of exhaustion and acute circulation insufficiency showed acute ultrastructural damage to the myocard and the skeletal muscle. These represent the morphological equivalent of a stress induced cardiomyopathy and are to be counted among the stress myopathies of the pig. These changes may be caused particularly by the high lactate production of the predominantly glycolytically operating skeletal muscles and by the effects of cardial catecholamine release following transport stress. Attention is drawn to the model character of these changes for similar processes in humans.", "contents": "Changes of cardiac and skeletal muscle in pigs following transport stress. An electron microscopic study. After lorry transport (60--120 min), fattening pigs (100--120 kg living weight) with clinical signs of exhaustion and acute circulation insufficiency showed acute ultrastructural damage to the myocard and the skeletal muscle. These represent the morphological equivalent of a stress induced cardiomyopathy and are to be counted among the stress myopathies of the pig. These changes may be caused particularly by the high lactate production of the predominantly glycolytically operating skeletal muscles and by the effects of cardial catecholamine release following transport stress. Attention is drawn to the model character of these changes for similar processes in humans."}
{"id": "PMID:477827", "title": "Morphological and biochemical changes induced in rat stomach by N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "Morphological and biochemical changes reflecting the process of genesis of experimental stomach cancer were studied and compared in experiments carried out on 170 rats to whom N-methyl-N'-nitro-N-nitrosoguanidine had been given in drinking water. The concentration of the compound in drinking water was 167 mg/l. Malignization mostly developed during the ingrowth of the epithelial complexes involved in the process of carcinogenesis into the submucous membrane, in rare cases, into the mucous membrane itself. The results of experiments showed that biochemical changes preceded morphological alterations. The signs of inhibition of the synthesis of the isoenzyme spectrum of pepsinogen-pepsin revealed the qualitative changes of biochemical processes of the epithelium. A possible dependence of morphological features of the process of carcinogenesis on the evolutionally-established functional peculiarities of rat stomach epithelium is discussed.", "contents": "Morphological and biochemical changes induced in rat stomach by N-methyl-N'-nitro-N-nitrosoguanidine. Morphological and biochemical changes reflecting the process of genesis of experimental stomach cancer were studied and compared in experiments carried out on 170 rats to whom N-methyl-N'-nitro-N-nitrosoguanidine had been given in drinking water. The concentration of the compound in drinking water was 167 mg/l. Malignization mostly developed during the ingrowth of the epithelial complexes involved in the process of carcinogenesis into the submucous membrane, in rare cases, into the mucous membrane itself. The results of experiments showed that biochemical changes preceded morphological alterations. The signs of inhibition of the synthesis of the isoenzyme spectrum of pepsinogen-pepsin revealed the qualitative changes of biochemical processes of the epithelium. A possible dependence of morphological features of the process of carcinogenesis on the evolutionally-established functional peculiarities of rat stomach epithelium is discussed."}
{"id": "PMID:477828", "title": "Synthesis and pharmacological properties of [1-N4-dimethyl-asparagine]-angiotensin II.", "content": "[1-N4-Dimethyl-asparagine]-angiotensin II was synthesized by Merrifield's solid-phase procedure. The analogue gave rat blood pressure about 70% relative potency to Hypertension (Ciba). Rabbit aorta strips gave intrinsic activity alpha E = 1, a PD2 of 6.92 +/- 0.09 and an affinity relative to [Asn1]-angiotensin II of 6.5%.", "contents": "Synthesis and pharmacological properties of [1-N4-dimethyl-asparagine]-angiotensin II. [1-N4-Dimethyl-asparagine]-angiotensin II was synthesized by Merrifield's solid-phase procedure. The analogue gave rat blood pressure about 70% relative potency to Hypertension (Ciba). Rabbit aorta strips gave intrinsic activity alpha E = 1, a PD2 of 6.92 +/- 0.09 and an affinity relative to [Asn1]-angiotensin II of 6.5%."}
{"id": "PMID:477829", "title": "Catalase and dehydroascorbate reductase in human polymorphonuclear leukocytes (PMN): possible functional relationship.", "content": "In leukocytes (PMN) of individuals with Swiss type acatalasemia, the rate of dehydroascorbate reduction is 4 times normal. This observation suggests that the protective function served by catalase in human PMN is supported by dehydroascorbate reductase.", "contents": "Catalase and dehydroascorbate reductase in human polymorphonuclear leukocytes (PMN): possible functional relationship. In leukocytes (PMN) of individuals with Swiss type acatalasemia, the rate of dehydroascorbate reduction is 4 times normal. This observation suggests that the protective function served by catalase in human PMN is supported by dehydroascorbate reductase."}
{"id": "PMID:477830", "title": "Selective and reversible modification of essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase by isothiocyanates.", "content": "Aralkyl isothiocyanates, like aryl isothiocyanates, undergo a selective and reversible reaction with essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase; the former 2 substances require for a reversible reaction course of more alkaline medium and presence of a thiol.", "contents": "Selective and reversible modification of essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase by isothiocyanates. Aralkyl isothiocyanates, like aryl isothiocyanates, undergo a selective and reversible reaction with essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase; the former 2 substances require for a reversible reaction course of more alkaline medium and presence of a thiol."}
{"id": "PMID:477831", "title": "Effect of clofibrate on the phospholipid biosynthesis in rat liver.", "content": "The effect of clofibrate on rat liver phospholipid biosynthesis was studied using 32P as a precursor. Phospholipid classes, levels and specific radioactivity were evaluated. Significant increases in levels of phosphatidylethanolamine and phosphatidylcholine were found and could account for the observed increase in total phospholipids. Specific activity of phosphatidylserine increased and that of phosphatidylethanolamine decreased. This fact suggests that clofibrate seems to alter the systems engaged in the transformation occurring within the different classes of phospholipids but not the de novo biosynthesis.", "contents": "Effect of clofibrate on the phospholipid biosynthesis in rat liver. The effect of clofibrate on rat liver phospholipid biosynthesis was studied using 32P as a precursor. Phospholipid classes, levels and specific radioactivity were evaluated. Significant increases in levels of phosphatidylethanolamine and phosphatidylcholine were found and could account for the observed increase in total phospholipids. Specific activity of phosphatidylserine increased and that of phosphatidylethanolamine decreased. This fact suggests that clofibrate seems to alter the systems engaged in the transformation occurring within the different classes of phospholipids but not the de novo biosynthesis."}
{"id": "PMID:477832", "title": "Artifacts from agar-protein interaction simulating a bacterial growth in the haemocultures.", "content": "Some morphological, histochemical and biochemical properties of the particles taking origin from the polysaccharide-proteins interaction in the presence of platelets and lymphocytes are described.", "contents": "Artifacts from agar-protein interaction simulating a bacterial growth in the haemocultures. Some morphological, histochemical and biochemical properties of the particles taking origin from the polysaccharide-proteins interaction in the presence of platelets and lymphocytes are described."}
{"id": "PMID:477833", "title": "Effect of splenectomy on the humoral immune response in the lizard, Scincus scincus.", "content": "Adult splenectomy in the lizard, Scincus scincus, did not affect humoral immune response to rat erythrocytes until 30 days post-immunization, but severely depressed subsequent antibody production.", "contents": "Effect of splenectomy on the humoral immune response in the lizard, Scincus scincus. Adult splenectomy in the lizard, Scincus scincus, did not affect humoral immune response to rat erythrocytes until 30 days post-immunization, but severely depressed subsequent antibody production."}
{"id": "PMID:477834", "title": "Cerebral ammonia production during hypoglycemia in the newborn calf.", "content": "The effect of insulin hypoglycaemia on cerebral blood flow, and cerebral metabolic rates of glucose, oxygen and ammonia was investigated in the unanaesthetized newborn calf. A net loss of ammonia from the brain occurred during hypoglycaemia, and was greater in convulsing than in comatose animals.", "contents": "Cerebral ammonia production during hypoglycemia in the newborn calf. The effect of insulin hypoglycaemia on cerebral blood flow, and cerebral metabolic rates of glucose, oxygen and ammonia was investigated in the unanaesthetized newborn calf. A net loss of ammonia from the brain occurred during hypoglycaemia, and was greater in convulsing than in comatose animals."}
{"id": "PMID:477835", "title": "Changes in efferent activities of the gastric vagus nerve by administration of glucose in the portal vein.", "content": "Injection of glucose in the portal vein, as well as in the left carotid artery, brought a transient decrease in efferent discharges of the gastric vagus nerve, whereas venous injection of regular insulin brought a gradual increase in the discharges.", "contents": "Changes in efferent activities of the gastric vagus nerve by administration of glucose in the portal vein. Injection of glucose in the portal vein, as well as in the left carotid artery, brought a transient decrease in efferent discharges of the gastric vagus nerve, whereas venous injection of regular insulin brought a gradual increase in the discharges."}
{"id": "PMID:477836", "title": "Semen copper in normal and infertile subjects.", "content": "Concentration of copper in seminal plasma was found to be less than that of normal in cases of oligospermia and azoospermia. It was more in oligoasthenospermia and asthenospermia when compared with that of normal. Chances of initiation of sperm motility by copper is discussed alongwith the inhibitory role it plays.", "contents": "Semen copper in normal and infertile subjects. Concentration of copper in seminal plasma was found to be less than that of normal in cases of oligospermia and azoospermia. It was more in oligoasthenospermia and asthenospermia when compared with that of normal. Chances of initiation of sperm motility by copper is discussed alongwith the inhibitory role it plays."}
{"id": "PMID:477837", "title": "Developmental change of EEG in rat from 4th to 16th week.", "content": "The development of EEG in 8 male rats from 4 to 16 weeks age were studied chronically. Theta band had the highest power at 5--16 weeks. Especially after 11 weeks, theta band presented a significantly higher peak than that of 4-week-old. In contrast to this, delta band, which had the highest power at 4 weeks, was markedly decreased.", "contents": "Developmental change of EEG in rat from 4th to 16th week. The development of EEG in 8 male rats from 4 to 16 weeks age were studied chronically. Theta band had the highest power at 5--16 weeks. Especially after 11 weeks, theta band presented a significantly higher peak than that of 4-week-old. In contrast to this, delta band, which had the highest power at 4 weeks, was markedly decreased."}
{"id": "PMID:477838", "title": "Formation of glomerulus-like structures by the olfactory nerve neonatal bulbectomy.", "content": "Evidence presented here suggests that glomerulus formation within the olfactory bulb of the rat, which is mostly a postnatal event, is directed by the olfactory nerve rather than by the influences of mitral and tufted cells.", "contents": "Formation of glomerulus-like structures by the olfactory nerve neonatal bulbectomy. Evidence presented here suggests that glomerulus formation within the olfactory bulb of the rat, which is mostly a postnatal event, is directed by the olfactory nerve rather than by the influences of mitral and tufted cells."}
{"id": "PMID:477839", "title": "Topographical distribution of ATP in rat brain.", "content": "Studies on the distribution of ATP in microdissected segments of the rat brain indicate that the nucleotide is concentrated in gray matter, and especially in the thalamus, hippocampus, entorhinal cortex and sensorimotor cortex. These distribution studies in conjunction with previous neuropharmacological studies, support the concept that adenine nucleotides may function as intercellular mediators in various regions of the brain.", "contents": "Topographical distribution of ATP in rat brain. Studies on the distribution of ATP in microdissected segments of the rat brain indicate that the nucleotide is concentrated in gray matter, and especially in the thalamus, hippocampus, entorhinal cortex and sensorimotor cortex. These distribution studies in conjunction with previous neuropharmacological studies, support the concept that adenine nucleotides may function as intercellular mediators in various regions of the brain."}
{"id": "PMID:477840", "title": "Facilitation of the dorsal root potentials by volleys spreading caudally and cranially in the spinal cord.", "content": "Conditioning volleys spreading along 3 segments of the cord produce larger facilitation of bilateral dorsal root potentials in sacral cord than volleys in neighbouring dorsal roots used to evoke the testing depolarization. Contralateral potentials are more facilitated by volleys descending from lumbar than ascending from caudal segments.", "contents": "Facilitation of the dorsal root potentials by volleys spreading caudally and cranially in the spinal cord. Conditioning volleys spreading along 3 segments of the cord produce larger facilitation of bilateral dorsal root potentials in sacral cord than volleys in neighbouring dorsal roots used to evoke the testing depolarization. Contralateral potentials are more facilitated by volleys descending from lumbar than ascending from caudal segments."}
{"id": "PMID:477841", "title": "Supernormal responses to premature stimulation in Ca-dependent action potentials.", "content": "The responses of Ca-dependent action potentials to premature stimulation were studied in the isolated canine ventricular muscle. When very premature stimuli were applied, supernormally augmented responses with propagation occurred, while responses with long preceding intervals were small and not conducted.", "contents": "Supernormal responses to premature stimulation in Ca-dependent action potentials. The responses of Ca-dependent action potentials to premature stimulation were studied in the isolated canine ventricular muscle. When very premature stimuli were applied, supernormally augmented responses with propagation occurred, while responses with long preceding intervals were small and not conducted."}
{"id": "PMID:477842", "title": "The effects of dopaminergic blocking agents on the glucose tolerance test in 6 humans and 6 dogs.", "content": "Pre-treatment with low doses of sulpiride, an atypical dopaminergic blocking agent, but not haloperidol, a classical dopaminergic blocking agent, decreased tolerance to glucose and increased blood serotonin levels in 6 normal humans and 6 normal dogs investigated.", "contents": "The effects of dopaminergic blocking agents on the glucose tolerance test in 6 humans and 6 dogs. Pre-treatment with low doses of sulpiride, an atypical dopaminergic blocking agent, but not haloperidol, a classical dopaminergic blocking agent, decreased tolerance to glucose and increased blood serotonin levels in 6 normal humans and 6 normal dogs investigated."}
{"id": "PMID:477843", "title": "Disparate Na+-requirement of taurocholate and indocyanine green uptake by isolated hepatocytes.", "content": "Uptake of both taurocholate and indocyanine green (ICG) by isolated rat hepatocytes was saturable and obeyed Michaelis-Menten kinetics. Only uptake of taurocholate exhibited Na+-dependence, a phenomenon compatible with active membrane transport. These findings suggest that the mechanisms responsible for hepatocellular uptake of bile acids and anionic dyes differ fundamentally.", "contents": "Disparate Na+-requirement of taurocholate and indocyanine green uptake by isolated hepatocytes. Uptake of both taurocholate and indocyanine green (ICG) by isolated rat hepatocytes was saturable and obeyed Michaelis-Menten kinetics. Only uptake of taurocholate exhibited Na+-dependence, a phenomenon compatible with active membrane transport. These findings suggest that the mechanisms responsible for hepatocellular uptake of bile acids and anionic dyes differ fundamentally."}
{"id": "PMID:477844", "title": "Effects of low doses of ochratoxin A after intratesticular injection in the rat.", "content": "The toxic effect of various doses of ochratoxin A on the rat testis was investigated after a single intratesticular injection. At time of sacrifice (day 10) degenerating changes occur in the testicular tissues: seminiferous tubules dilatation, cytolysis of the seminiferous epithelium, hyperplasia of the interstitial tissue, vascular thrombosis. The relations between the blood supply disturbances and the observed lesions are discussed.", "contents": "Effects of low doses of ochratoxin A after intratesticular injection in the rat. The toxic effect of various doses of ochratoxin A on the rat testis was investigated after a single intratesticular injection. At time of sacrifice (day 10) degenerating changes occur in the testicular tissues: seminiferous tubules dilatation, cytolysis of the seminiferous epithelium, hyperplasia of the interstitial tissue, vascular thrombosis. The relations between the blood supply disturbances and the observed lesions are discussed."}
{"id": "PMID:477845", "title": "Attenuation by carbocromen of cardiac metabolism alterations due to ischemia.", "content": "Carbocromen prevents to some extent, particularly in subendocardial layer, carbohydrate cardiac metabolism alterations induced by the ischemia obtained by intermittent occlusion of left coronary artery.", "contents": "Attenuation by carbocromen of cardiac metabolism alterations due to ischemia. Carbocromen prevents to some extent, particularly in subendocardial layer, carbohydrate cardiac metabolism alterations induced by the ischemia obtained by intermittent occlusion of left coronary artery."}
{"id": "PMID:477846", "title": "Hashish smoke interfers with Sidman avoidance in mice.", "content": "Hashish smoke has been proved to be active in the Sidman avoidance. Its activity is similar to that of hallucinogens.", "contents": "Hashish smoke interfers with Sidman avoidance in mice. Hashish smoke has been proved to be active in the Sidman avoidance. Its activity is similar to that of hallucinogens."}
{"id": "PMID:477847", "title": "Possible stimulatory effect of retinoic acid on pulmonary macrophages.", "content": "Retinoic acid was administered to hamsters suffering from N-nitroso-N-methylurethane-induced fibrosing alveolitis. A significant increase in macrophage numbers was seen in the lungs of retinoid-treated animals as compared to the unsupplemented group.", "contents": "Possible stimulatory effect of retinoic acid on pulmonary macrophages. Retinoic acid was administered to hamsters suffering from N-nitroso-N-methylurethane-induced fibrosing alveolitis. A significant increase in macrophage numbers was seen in the lungs of retinoid-treated animals as compared to the unsupplemented group."}
{"id": "PMID:477848", "title": "Nuclear protrusions in malignant tumours with large abnormal chromosomes: observations on C-banded preparations.", "content": "The appearances are described in 4 human tumours having nuclear protrusions associated with large abnormal chromosomes. In C-banded preparations, chromocentres were seen in the protrusions only where interstitial C-bands were present on the long arm of the abnormal chromosome, providing evidence that the protrusions are indeed formed by the long arms.", "contents": "Nuclear protrusions in malignant tumours with large abnormal chromosomes: observations on C-banded preparations. The appearances are described in 4 human tumours having nuclear protrusions associated with large abnormal chromosomes. In C-banded preparations, chromocentres were seen in the protrusions only where interstitial C-bands were present on the long arm of the abnormal chromosome, providing evidence that the protrusions are indeed formed by the long arms."}
{"id": "PMID:477849", "title": "Hyperosmolar coma as etiological factor in the CNS radiation syndrome of rats.", "content": "Supralethal dose of whole-body or trunk but not head-irradiation in rats induced hyperosmolar coma accompanied by hypernatremia and hyperkalemia. This clinical entity has presented the symptoms of the CNS radiation syndrome with a characteristic short survival time.", "contents": "Hyperosmolar coma as etiological factor in the CNS radiation syndrome of rats. Supralethal dose of whole-body or trunk but not head-irradiation in rats induced hyperosmolar coma accompanied by hypernatremia and hyperkalemia. This clinical entity has presented the symptoms of the CNS radiation syndrome with a characteristic short survival time."}
{"id": "PMID:477850", "title": "125I-Insulin is preferentially internalized in regions of the hepatocytes rich in lysosomal and Golgi structures.", "content": "125I-Insulin initially localizes to the plasma membrane of isolated rat hepatocytes but is subsequently internalized and preferentially associates with lysosomal structures. In the present study, we show that this preferential association to lysosomes occurs in regions of the cell rich in lysosomal and Golgi structures.", "contents": "125I-Insulin is preferentially internalized in regions of the hepatocytes rich in lysosomal and Golgi structures. 125I-Insulin initially localizes to the plasma membrane of isolated rat hepatocytes but is subsequently internalized and preferentially associates with lysosomal structures. In the present study, we show that this preferential association to lysosomes occurs in regions of the cell rich in lysosomal and Golgi structures."}
{"id": "PMID:477852", "title": "Plasma prostaglandin levels in fed and starved lean, normal and obese women.", "content": "The plasma obtained from fed and starved lean, normal and obese women was estimated, by a radio-immunoassay method, for prostaglandins, owing to their implication in the regulation of adipose tissue lipolysis and the development of obesity. No significant differences were found due to nutritional status or body-build. However, a significantly higher plasma concentration of prostaglandins of the E-type than of the F-type, was found consistently. The very low levels of prostaglandins observed (a range of 0.10--0.15 ng ml-1 for E-type and a range of 0.05--0.07 ng ml-1 for the F-type) may be due, in part, to the activity of a plasmatic prostaglandin metabolizing system.", "contents": "Plasma prostaglandin levels in fed and starved lean, normal and obese women. The plasma obtained from fed and starved lean, normal and obese women was estimated, by a radio-immunoassay method, for prostaglandins, owing to their implication in the regulation of adipose tissue lipolysis and the development of obesity. No significant differences were found due to nutritional status or body-build. However, a significantly higher plasma concentration of prostaglandins of the E-type than of the F-type, was found consistently. The very low levels of prostaglandins observed (a range of 0.10--0.15 ng ml-1 for E-type and a range of 0.05--0.07 ng ml-1 for the F-type) may be due, in part, to the activity of a plasmatic prostaglandin metabolizing system."}
{"id": "PMID:477853", "title": "A micro-electrode amplifier with an infinite resistance current source for intracellular measurements of membrane potential and resistance changes under current clamp.", "content": "A microelectrode amplifier for intracellular electrophysiological research is described. It is equipped with an electronic infinite resistance constant current source for the injection of current into biological cells. With this amplifier the potential changes, the resistance changes and the dependence on extrinsic current of single cells can be measured independently and simultaneously.", "contents": "A micro-electrode amplifier with an infinite resistance current source for intracellular measurements of membrane potential and resistance changes under current clamp. A microelectrode amplifier for intracellular electrophysiological research is described. It is equipped with an electronic infinite resistance constant current source for the injection of current into biological cells. With this amplifier the potential changes, the resistance changes and the dependence on extrinsic current of single cells can be measured independently and simultaneously."}
{"id": "PMID:477851", "title": "[Effect of Corynebacterium parvum on serum lysozyme (muramidase) levels (author's transl)].", "content": "An i.v. injection of 548 microgram of killed Corynebacterium parvum into C57B1 mice leads to significant changes in serum lysozyme (muramidase) levels. After an initial fall at 24 h, the activity of the enzyme increased progressively, reached a peak on the 9th day and returned to control range after the 15th day.", "contents": "[Effect of Corynebacterium parvum on serum lysozyme (muramidase) levels (author's transl)]. An i.v. injection of 548 microgram of killed Corynebacterium parvum into C57B1 mice leads to significant changes in serum lysozyme (muramidase) levels. After an initial fall at 24 h, the activity of the enzyme increased progressively, reached a peak on the 9th day and returned to control range after the 15th day."}
{"id": "PMID:477854", "title": "Mitochondrial calcium efflux and porcine stress-susceptibility.", "content": "Mitchondrial Ca2+ efflux rates of M. longissimus dorsi correlate very closely with parameters associated with porcine stress-susceptibility. Experimental data support the measurement of mitochondrial Ca+2 efflux to be a very sensitive and reliable method for differentiating porcine stress-susceptibility.", "contents": "Mitochondrial calcium efflux and porcine stress-susceptibility. Mitchondrial Ca2+ efflux rates of M. longissimus dorsi correlate very closely with parameters associated with porcine stress-susceptibility. Experimental data support the measurement of mitochondrial Ca+2 efflux to be a very sensitive and reliable method for differentiating porcine stress-susceptibility."}
{"id": "PMID:477855", "title": "Evidence for dissociation of ferrihemoglobin by poly-L-lysine.", "content": "Circular dichroism and absorption spectra of ferrihemoglobin were shown to be altered upon binding with poly-L-lysine at alkaline pH. When ferrihemoglobin immobilized to Sepharose gel was treated with poly-L-lysine, hemoglobin subunits were released from the gel. These results suggest that ferrihemoglobin was dissociated into subunits by poly-L-lysine.", "contents": "Evidence for dissociation of ferrihemoglobin by poly-L-lysine. Circular dichroism and absorption spectra of ferrihemoglobin were shown to be altered upon binding with poly-L-lysine at alkaline pH. When ferrihemoglobin immobilized to Sepharose gel was treated with poly-L-lysine, hemoglobin subunits were released from the gel. These results suggest that ferrihemoglobin was dissociated into subunits by poly-L-lysine."}
{"id": "PMID:477856", "title": "A high molecular weight inhibitor of Ca2+ -dependent neutral protease in rat brain.", "content": "An endogenous, heat-stable inhibitor of high mol. wt (approximately 3x10(5)) was found to be present in rat brain, which inhibited Ca2+-dependent neutral protease specifically but not due to its binding of Ca2+ in the medium .", "contents": "A high molecular weight inhibitor of Ca2+ -dependent neutral protease in rat brain. An endogenous, heat-stable inhibitor of high mol. wt (approximately 3x10(5)) was found to be present in rat brain, which inhibited Ca2+-dependent neutral protease specifically but not due to its binding of Ca2+ in the medium ."}
{"id": "PMID:477857", "title": "Binding capacities of various analogues of S-adenosyl-L-homocysteine to protein methyltransferase II from human erythrocytes.", "content": "A series of analogues of S-adenosyl-L-homocysteine, modified mainly in the amino acid portion of the molecule, have been synthesized. All were found to be competitive inhibitors of protein methyltransferase II from human erythrocytes. S-adenosyl-L-homocysteine remains however by far the most effective inhibitor of the methylase.", "contents": "Binding capacities of various analogues of S-adenosyl-L-homocysteine to protein methyltransferase II from human erythrocytes. A series of analogues of S-adenosyl-L-homocysteine, modified mainly in the amino acid portion of the molecule, have been synthesized. All were found to be competitive inhibitors of protein methyltransferase II from human erythrocytes. S-adenosyl-L-homocysteine remains however by far the most effective inhibitor of the methylase."}
{"id": "PMID:477858", "title": "Inversal relationship between basal adenylcyclase activity and maximal degree of stimulation in membranes of red blood cells from rats.", "content": "The degree of stimulation of adenylcyclase activity, in membranes from immature red blood cells from rats, brought about by isoprenaline, guanylyl-imidodiphosphate and sodium fluoride is strongly dependent on the basal activity of the enzyme. The inversal relationship between basal activity and the maximal degree of stimulation by (--) isoprenaline, shows an apparent seasonal dependence.", "contents": "Inversal relationship between basal adenylcyclase activity and maximal degree of stimulation in membranes of red blood cells from rats. The degree of stimulation of adenylcyclase activity, in membranes from immature red blood cells from rats, brought about by isoprenaline, guanylyl-imidodiphosphate and sodium fluoride is strongly dependent on the basal activity of the enzyme. The inversal relationship between basal activity and the maximal degree of stimulation by (--) isoprenaline, shows an apparent seasonal dependence."}
{"id": "PMID:477859", "title": "Inhibition of alpha-chymotrypsin by 2-halogeno-ethanols; comparisons with 2-methyl-ethanols and urea.", "content": "2-Halogeno- and 2-methyl-ethanols inhibit alpha-chymotrypsin in the order of their substituted groups: [1] tri- greater than di- greater than mono-, [2] Br- greater than Cl- greater than CH3- greater than F-. The inhibition by the halogeno-ethanols is mediated differently from that by the methyl-ethanols, ethanol, and urea.", "contents": "Inhibition of alpha-chymotrypsin by 2-halogeno-ethanols; comparisons with 2-methyl-ethanols and urea. 2-Halogeno- and 2-methyl-ethanols inhibit alpha-chymotrypsin in the order of their substituted groups: [1] tri- greater than di- greater than mono-, [2] Br- greater than Cl- greater than CH3- greater than F-. The inhibition by the halogeno-ethanols is mediated differently from that by the methyl-ethanols, ethanol, and urea."}
{"id": "PMID:477860", "title": "[Rat liver S-adenosyl-L-homocysteine hydrolase purification by affinity column chromatography (author's transl)].", "content": "S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1) has been purified 240-fold from rat liver by affinity column chromatography on aminohexyl sepharose bound 6-mercaptopurine 9 D-riboside. The purified enzyme was homogeneous by gel electrophoresis.", "contents": "[Rat liver S-adenosyl-L-homocysteine hydrolase purification by affinity column chromatography (author's transl)]. S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1) has been purified 240-fold from rat liver by affinity column chromatography on aminohexyl sepharose bound 6-mercaptopurine 9 D-riboside. The purified enzyme was homogeneous by gel electrophoresis."}
{"id": "PMID:477861", "title": "A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.", "content": "A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.", "contents": "A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate. A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase."}
{"id": "PMID:477862", "title": "Position of the nucleolus within the nuclei of pachytene spermatocytes of Dromiciops australis and Marmosa elegans (Didelphoidea-Marsupialia).", "content": "The location of the nucleoli within the nuclei of pachytene spermatocytes, and their relation with the position of the nucleolar organizer region (NOR) was studied. It appears that a terminal NOR determines a peripheral location of the nucleolus, due to the position of the NOR over the synaptonemal complex and to the attachment of the nucleolar chromosome telomeres at the nuclear membrane.", "contents": "Position of the nucleolus within the nuclei of pachytene spermatocytes of Dromiciops australis and Marmosa elegans (Didelphoidea-Marsupialia). The location of the nucleoli within the nuclei of pachytene spermatocytes, and their relation with the position of the nucleolar organizer region (NOR) was studied. It appears that a terminal NOR determines a peripheral location of the nucleolus, due to the position of the NOR over the synaptonemal complex and to the attachment of the nucleolar chromosome telomeres at the nuclear membrane."}
{"id": "PMID:477863", "title": "Presence of viruses in a strain of mycoplasma pulmonis.", "content": "Filtrates prepared from heavily grown agar cultures of M. pulmonis strain Negroni-52 formed plaques on lawns of A. laidlawii strain JAl but not on those of M. pulmonis strains Ash or Negroni-52. The plaque-forming agent proved to be rod-shaped particles morphologically identical with mycoplasmavirus group 1. Evidence supporting the conclusion that the virus originated from Negroni-52 has been obtained. Electron microscopy revelaed that Negroni-52 is also a carrier of long-tailed phage-like particles.", "contents": "Presence of viruses in a strain of mycoplasma pulmonis. Filtrates prepared from heavily grown agar cultures of M. pulmonis strain Negroni-52 formed plaques on lawns of A. laidlawii strain JAl but not on those of M. pulmonis strains Ash or Negroni-52. The plaque-forming agent proved to be rod-shaped particles morphologically identical with mycoplasmavirus group 1. Evidence supporting the conclusion that the virus originated from Negroni-52 has been obtained. Electron microscopy revelaed that Negroni-52 is also a carrier of long-tailed phage-like particles."}
{"id": "PMID:477864", "title": "[Lipids in the egg shell of the ostrich (Struthio camelus) (author's transl)].", "content": "The egg shell (with cuticle) of the ostrich contained a total lipid concentration of 0.19 mg/g egg shell; the phospholipids constituted the major portion, among the neutral' lipids cholesterol esters, cholesterol and free fatty acids were identified. The egg shell lipids showed a stimulating effect on the crystal growth of calcium carbonate; the highest rate of crystal growth was observed with the phospholipid fraction.", "contents": "[Lipids in the egg shell of the ostrich (Struthio camelus) (author's transl)]. The egg shell (with cuticle) of the ostrich contained a total lipid concentration of 0.19 mg/g egg shell; the phospholipids constituted the major portion, among the neutral' lipids cholesterol esters, cholesterol and free fatty acids were identified. The egg shell lipids showed a stimulating effect on the crystal growth of calcium carbonate; the highest rate of crystal growth was observed with the phospholipid fraction."}
{"id": "PMID:477865", "title": "Certain biochemical changes in the ovary of a migratory bird, Calandrella acutirostris tibetana.", "content": "The changes in the ovarian cholesterol, glycogen and ascorbic acid of Bagheri, Calandrella acutirostris tibetana, during its non-breeding and breeding periods were studied. There was a significant increase in all the 3 biochemical constituents during the breeding period.", "contents": "Certain biochemical changes in the ovary of a migratory bird, Calandrella acutirostris tibetana. The changes in the ovarian cholesterol, glycogen and ascorbic acid of Bagheri, Calandrella acutirostris tibetana, during its non-breeding and breeding periods were studied. There was a significant increase in all the 3 biochemical constituents during the breeding period."}
{"id": "PMID:477866", "title": "Changes in photoelectron transport of chloroplasts isolated from dark stressed leaves of maize seedlings.", "content": "Transfer of light-grown maize seedlings to dark causes a loss in the the contents of chlorophyll, protein and RNA of leaves, and a decline in DCPIP photoreduction by isolated chloroplasts. The loss in DCPIP-Hill reaction is attributed to the dark stress-induced damage of O2 evolving system of thylakoid membranes.", "contents": "Changes in photoelectron transport of chloroplasts isolated from dark stressed leaves of maize seedlings. Transfer of light-grown maize seedlings to dark causes a loss in the the contents of chlorophyll, protein and RNA of leaves, and a decline in DCPIP photoreduction by isolated chloroplasts. The loss in DCPIP-Hill reaction is attributed to the dark stress-induced damage of O2 evolving system of thylakoid membranes."}
{"id": "PMID:477867", "title": "Rapid, quantitative adipose conversion of chicken fibroblasts by high concentrations of chicken serum or plasma.", "content": "Rapid, quantitative adipose conversion of chicken fibroblasts occurs when these cells are cultured in undiluted commercial chicken serum or plasma. Fresh serum and plasma acquire this property after ageing.", "contents": "Rapid, quantitative adipose conversion of chicken fibroblasts by high concentrations of chicken serum or plasma. Rapid, quantitative adipose conversion of chicken fibroblasts occurs when these cells are cultured in undiluted commercial chicken serum or plasma. Fresh serum and plasma acquire this property after ageing."}
{"id": "PMID:477868", "title": "An actin-destabilizing factor is present in human plasma.", "content": "Plasma and serum of humans or experimental animals contain a factor which destabilizes F-actin. The factor has no DNAse or thrombin activity and after incubation with F-actin does not modify the position of the actin band on a SDS polyacrylamide gel. Hence it probably depolymerizes F-actin.", "contents": "An actin-destabilizing factor is present in human plasma. Plasma and serum of humans or experimental animals contain a factor which destabilizes F-actin. The factor has no DNAse or thrombin activity and after incubation with F-actin does not modify the position of the actin band on a SDS polyacrylamide gel. Hence it probably depolymerizes F-actin."}
{"id": "PMID:477869", "title": "Differential alterations in branched-chain amino acid decarboxylation in liver of hypophysectomized rats.", "content": "Valine decarboxylation was significantly increased and leucine decarboxylation was significantly decreased in rat liver slices following hypophysectomy. In both normal and hypophysectomized rats decarboxylation of leucine exceeded that of valine in slices whereas the reverse was observed with the respective keto acids and mitochondria.", "contents": "Differential alterations in branched-chain amino acid decarboxylation in liver of hypophysectomized rats. Valine decarboxylation was significantly increased and leucine decarboxylation was significantly decreased in rat liver slices following hypophysectomy. In both normal and hypophysectomized rats decarboxylation of leucine exceeded that of valine in slices whereas the reverse was observed with the respective keto acids and mitochondria."}
{"id": "PMID:477870", "title": "Studies of the blood of Ascidia ceratodes. Total blood cell counts, differential blood cell counts, hematocrit values, seasonal variations, and fluorescent characteristics of blood cells.", "content": "Seasonal, and animal size and weight variations of the blood cells of the vanadium-containing ascidian. Ascidia ceratodes, were determined. The fluorescent properties of various cell types were ascertained, and discussed in terms of cell development, phylogenic position of the species, and chemicals in the cells.", "contents": "Studies of the blood of Ascidia ceratodes. Total blood cell counts, differential blood cell counts, hematocrit values, seasonal variations, and fluorescent characteristics of blood cells. Seasonal, and animal size and weight variations of the blood cells of the vanadium-containing ascidian. Ascidia ceratodes, were determined. The fluorescent properties of various cell types were ascertained, and discussed in terms of cell development, phylogenic position of the species, and chemicals in the cells."}
{"id": "PMID:477871", "title": "Computer-aided biochemical system analysis in open systems with environment simulation.", "content": "A computer-aided arrangement is described which allows kinetic and regulative studies with enzymes, organelles and cells in an open system. This is demonstrated with some simple examples.", "contents": "Computer-aided biochemical system analysis in open systems with environment simulation. A computer-aided arrangement is described which allows kinetic and regulative studies with enzymes, organelles and cells in an open system. This is demonstrated with some simple examples."}
{"id": "PMID:477872", "title": "Dependence on the lipophilicity of maleimide derivatives in their inhibitory action upon chemotaxis in neutrophils.", "content": "Modification of polymorphonuclear neutrophils by a series of maleimide derivatives with various degrees of lipophilicity and hydrophilicity indicated that hydrophilic reagents had little effect on chemotaxis, whereas the degree of the inhibitory effect of lipophilic reagents on the chemotaxis was parallel to the degree of their lipophilicity.", "contents": "Dependence on the lipophilicity of maleimide derivatives in their inhibitory action upon chemotaxis in neutrophils. Modification of polymorphonuclear neutrophils by a series of maleimide derivatives with various degrees of lipophilicity and hydrophilicity indicated that hydrophilic reagents had little effect on chemotaxis, whereas the degree of the inhibitory effect of lipophilic reagents on the chemotaxis was parallel to the degree of their lipophilicity."}
{"id": "PMID:477873", "title": "Effect of chronic hypo and hypervitaminosis C on the brush border enzymes and the intestinal uptake of glucose and alanine.", "content": "Brush border sucrase and alkaline phosphatase activities are considerably enhanced in the intestine of ascorbic acid deficient guinea-pigs. Similar increase in the uptake of D-glucose and L-alanine also occurs in chronic vitamin C deficiency. However the permeability of D-glucose and L-alanine in the intestine of animals fed with large doses of vitamin C is severely depressed, with a reduction in the levels of sucrase and alkaline phosphatase activities.", "contents": "Effect of chronic hypo and hypervitaminosis C on the brush border enzymes and the intestinal uptake of glucose and alanine. Brush border sucrase and alkaline phosphatase activities are considerably enhanced in the intestine of ascorbic acid deficient guinea-pigs. Similar increase in the uptake of D-glucose and L-alanine also occurs in chronic vitamin C deficiency. However the permeability of D-glucose and L-alanine in the intestine of animals fed with large doses of vitamin C is severely depressed, with a reduction in the levels of sucrase and alkaline phosphatase activities."}
{"id": "PMID:477874", "title": "Positive alliesthesia after insulin.", "content": "Volunteers experienced sucrose solution as more pleasant 36-48 min after insulin, than after saline control. These changes in affective estimates correlate negatively with blood sugar at 30 min and positively at 50 min after the insulin injection.", "contents": "Positive alliesthesia after insulin. Volunteers experienced sucrose solution as more pleasant 36-48 min after insulin, than after saline control. These changes in affective estimates correlate negatively with blood sugar at 30 min and positively at 50 min after the insulin injection."}
{"id": "PMID:477875", "title": "Effect of prolactin on fluid and NaCl absorption by the rat proximal and distal colon.", "content": "The rates of fluid and NaCl absorption are greater in the proximal than in the distal colon. Prolactin treatment caused significant increases in fluid and NaCl absorption in the proximal but not in the distal colon. This suggests that only the proximal colon region, where most of the fluid and sodium absorption takes place, is responsive to prolactin.", "contents": "Effect of prolactin on fluid and NaCl absorption by the rat proximal and distal colon. The rates of fluid and NaCl absorption are greater in the proximal than in the distal colon. Prolactin treatment caused significant increases in fluid and NaCl absorption in the proximal but not in the distal colon. This suggests that only the proximal colon region, where most of the fluid and sodium absorption takes place, is responsive to prolactin."}
{"id": "PMID:477876", "title": "Physiological dissection of various effects of ruthenium red dye on Paramecium cells.", "content": "Polycationic dye ruthenium red, but not alcian blue, if externally applied to Paramecium cells quickly inhibits their phagocytosis. Ruthenium red combined with the cell surface diminishes frequency and duration of ciliary reversals and gradually inactivates the Ca++ gating mechanism. This effect persists for 1-3 after ruthenium red removal from the culture medium.", "contents": "Physiological dissection of various effects of ruthenium red dye on Paramecium cells. Polycationic dye ruthenium red, but not alcian blue, if externally applied to Paramecium cells quickly inhibits their phagocytosis. Ruthenium red combined with the cell surface diminishes frequency and duration of ciliary reversals and gradually inactivates the Ca++ gating mechanism. This effect persists for 1-3 after ruthenium red removal from the culture medium."}
{"id": "PMID:477877", "title": "Hematocrit as an index of changes in plasma volume in conscious dogs.", "content": "Hematocrit (HCT) measurements were made in intact and splenectomized conscious dogs to determine if observed decreases in HCT were produced by plasma volume expansion or splenic sequestration of erythrocytes. We found that in conscious dogs HCT is a poor indicator of changes in plasma volume.", "contents": "Hematocrit as an index of changes in plasma volume in conscious dogs. Hematocrit (HCT) measurements were made in intact and splenectomized conscious dogs to determine if observed decreases in HCT were produced by plasma volume expansion or splenic sequestration of erythrocytes. We found that in conscious dogs HCT is a poor indicator of changes in plasma volume."}
{"id": "PMID:477878", "title": "Retinoscopy and chromatic aberration.", "content": "The origin of the apparent farsightedness as revealed by retinoscopy in smaller eyes was investigated by using monochromatic retinoscopy on wild rabbits. Our results indicate that a combination of long wavelength light and chromatic aberration of the subject's dioptrics is the major source of this artifact.", "contents": "Retinoscopy and chromatic aberration. The origin of the apparent farsightedness as revealed by retinoscopy in smaller eyes was investigated by using monochromatic retinoscopy on wild rabbits. Our results indicate that a combination of long wavelength light and chromatic aberration of the subject's dioptrics is the major source of this artifact."}
{"id": "PMID:477879", "title": "Stimulation of gastric secretion by prostaglandin F2 alpha in rats.", "content": "In rats with chronic gastric fistulas, prostaglandin F2 alpha stimulated the gastric acid secretion in graded doses of 50, 100, 200 and 400 microgram/kg b.wt, while higher doses above 1 mg/kg b.wt tended to inhibit significantly. The gastric antisecretory effect of prostaglandin E1 could not be altered or modified by subsequent treatment of prostaglandin F2 alpha, while the latter alone without any prior treatment of the former, stimulated output of gastric juice, HCl and pepsin without significantly affecting the concentration of these components.", "contents": "Stimulation of gastric secretion by prostaglandin F2 alpha in rats. In rats with chronic gastric fistulas, prostaglandin F2 alpha stimulated the gastric acid secretion in graded doses of 50, 100, 200 and 400 microgram/kg b.wt, while higher doses above 1 mg/kg b.wt tended to inhibit significantly. The gastric antisecretory effect of prostaglandin E1 could not be altered or modified by subsequent treatment of prostaglandin F2 alpha, while the latter alone without any prior treatment of the former, stimulated output of gastric juice, HCl and pepsin without significantly affecting the concentration of these components."}
{"id": "PMID:477880", "title": "Effect of scopolamine on maze learning performance in humans.", "content": "Scopolamine was administered orally to volunteers who were required to learn a digit memory task and a tactile maze task. Comparison of their performance with that under control drugs suggests that blockage of central cholinergic synapses may have a larger effect on spatial memory than on nonspatial memory. Subjects tended to make more errors under scopolamine and to insert extra turns in drawings of the maze.", "contents": "Effect of scopolamine on maze learning performance in humans. Scopolamine was administered orally to volunteers who were required to learn a digit memory task and a tactile maze task. Comparison of their performance with that under control drugs suggests that blockage of central cholinergic synapses may have a larger effect on spatial memory than on nonspatial memory. Subjects tended to make more errors under scopolamine and to insert extra turns in drawings of the maze."}
{"id": "PMID:477881", "title": "The postnatal evolution of muscular twitches in the developing rat.", "content": "The reported study concerns the evolution of muscular twitches during the 21 postnatal days of the normal rat pups. The data indicate an age-dependent progression of these twitches in different body regions.", "contents": "The postnatal evolution of muscular twitches in the developing rat. The reported study concerns the evolution of muscular twitches during the 21 postnatal days of the normal rat pups. The data indicate an age-dependent progression of these twitches in different body regions."}
{"id": "PMID:477882", "title": "Spatial distribution of the adaptation field of the surround response mechanisms in type X cat retinal ganglion cells.", "content": "The surround response mechanism in on-center X-cells in cat retina was found to be bimodally distributed and weak or nonexistent in the receptive field middle. An on-inhibition measure was used to assess surround mechanism gain.", "contents": "Spatial distribution of the adaptation field of the surround response mechanisms in type X cat retinal ganglion cells. The surround response mechanism in on-center X-cells in cat retina was found to be bimodally distributed and weak or nonexistent in the receptive field middle. An on-inhibition measure was used to assess surround mechanism gain."}
{"id": "PMID:477883", "title": "Unsaturated lipid droplets in liver sinusoids lining cells in a case of Reye-Morgan-Baral syndrome.", "content": "The author reports a case of Reye-Morgan-Baral syndrome presenting osmiophilic-dense lipidic bodies in lining cells of hepatic sinusoids. The different lipidic saturation level between the content of these bodies and the one of lipid droplets of hepatocytes could represent a different response, between hepatocytes and lining cells of liver sinusoids, to excessive circulating lipids.", "contents": "Unsaturated lipid droplets in liver sinusoids lining cells in a case of Reye-Morgan-Baral syndrome. The author reports a case of Reye-Morgan-Baral syndrome presenting osmiophilic-dense lipidic bodies in lining cells of hepatic sinusoids. The different lipidic saturation level between the content of these bodies and the one of lipid droplets of hepatocytes could represent a different response, between hepatocytes and lining cells of liver sinusoids, to excessive circulating lipids."}
{"id": "PMID:477884", "title": "Effect of ionic environment on densities of membrane-associated particles in presynaptic membranes observed in freeze-fractured synaptosomes.", "content": "The effects of high-K and high-Ca in the incubation medium on membrane-associated particles of the presynaptic membranes were examined. There was a marked increase in the density of protoplasmic fracture face after incubation in the high-K or high-Ca medium.", "contents": "Effect of ionic environment on densities of membrane-associated particles in presynaptic membranes observed in freeze-fractured synaptosomes. The effects of high-K and high-Ca in the incubation medium on membrane-associated particles of the presynaptic membranes were examined. There was a marked increase in the density of protoplasmic fracture face after incubation in the high-K or high-Ca medium."}
{"id": "PMID:477885", "title": "The 'mauve factor' of schizophrenia and porphyria, 5-hydroxyhaemopyrrole lactam, has low pharmacological potency on guinea-pig ileum.", "content": "5-Hydroxyhaemopyrrole lactam, the 'mauve factor' reported in the urine of schizophrenics and porphyrics was found to inhibit electrically-stimulated contractions of guinea-pig ileum only at high concentrations (ID50 = 8.5 mM). This low potency makes it unlikely that the compound can account for neurotoxic effects in human porphyria.", "contents": "The 'mauve factor' of schizophrenia and porphyria, 5-hydroxyhaemopyrrole lactam, has low pharmacological potency on guinea-pig ileum. 5-Hydroxyhaemopyrrole lactam, the 'mauve factor' reported in the urine of schizophrenics and porphyrics was found to inhibit electrically-stimulated contractions of guinea-pig ileum only at high concentrations (ID50 = 8.5 mM). This low potency makes it unlikely that the compound can account for neurotoxic effects in human porphyria."}
{"id": "PMID:477886", "title": "Alteration of plasma ketamine levels in mice by probenecid.", "content": "Probenecid increases plasma ketamine levels in mice concurrently with an enhancement of duration of loss of righting reflex. The magnitude of these changes is directly related to the dose of ketamine and intervals between administration of compounds. Plasma levels of anesthetic are similar for all animals upon termination of hypnosis.", "contents": "Alteration of plasma ketamine levels in mice by probenecid. Probenecid increases plasma ketamine levels in mice concurrently with an enhancement of duration of loss of righting reflex. The magnitude of these changes is directly related to the dose of ketamine and intervals between administration of compounds. Plasma levels of anesthetic are similar for all animals upon termination of hypnosis."}
{"id": "PMID:477887", "title": "Haemagglutinating activity of modeccin.", "content": "Modeccin, the toxin of Adenia digitata, agglutinates erythrocytes from several mammalian species. The haemagglutinating activity is enhanced by neuraminidase and is inhibited by galactose and by galactose-containing sugars.", "contents": "Haemagglutinating activity of modeccin. Modeccin, the toxin of Adenia digitata, agglutinates erythrocytes from several mammalian species. The haemagglutinating activity is enhanced by neuraminidase and is inhibited by galactose and by galactose-containing sugars."}
{"id": "PMID:477888", "title": "Activation by S-adenosylhomocysteine of norepinephrine and serotonin in vitro uptake in synaptosomal preparations from rat brain.", "content": "S-Adenosylhomocysteine (10(-7)-10(-5) M) activated norepinephrine (NE) and serotonin (5HT) in vitro uptake in synaptosomal preparations from rat brain, but did not affect dopamine (DA) uptake. When administered to rats (7 mg/kg i.p.), it has the same effect on in vitro NE and 5HT uptake. It did not affect NE and 5HT release.", "contents": "Activation by S-adenosylhomocysteine of norepinephrine and serotonin in vitro uptake in synaptosomal preparations from rat brain. S-Adenosylhomocysteine (10(-7)-10(-5) M) activated norepinephrine (NE) and serotonin (5HT) in vitro uptake in synaptosomal preparations from rat brain, but did not affect dopamine (DA) uptake. When administered to rats (7 mg/kg i.p.), it has the same effect on in vitro NE and 5HT uptake. It did not affect NE and 5HT release."}
{"id": "PMID:477889", "title": "Radioprotective effect of a protein free parathyroid extract on the mitotic index of rat bone marrow cells.", "content": "The protective efficacy of an orally administered bovine protein-free parathyroid extract (PF-PTE) was studied on rat bone marrow cells in vivo with the mitotic index after 850 R irradiation. A remarkable decrease was found in the mitotic activity of bone marrow cells after irradiation in the non-protected animals. However, in the animals treated with PF-PTE after irradiation, a significantly smaller decrease and a faster recovery were found in the mitotic activity of the bone marrow cells.", "contents": "Radioprotective effect of a protein free parathyroid extract on the mitotic index of rat bone marrow cells. The protective efficacy of an orally administered bovine protein-free parathyroid extract (PF-PTE) was studied on rat bone marrow cells in vivo with the mitotic index after 850 R irradiation. A remarkable decrease was found in the mitotic activity of bone marrow cells after irradiation in the non-protected animals. However, in the animals treated with PF-PTE after irradiation, a significantly smaller decrease and a faster recovery were found in the mitotic activity of the bone marrow cells."}
{"id": "PMID:477890", "title": "The number of parallel fibre-Purkinje dendrite synapses. A morphometric evaluation.", "content": "Quantitative parameters concerning synapses were studied in the cerebellar molecular layer of 4 cats using ultrastructural morphometric methods. The number of parallel fibre-Purkinje dendrite synapses was estimated to be about 200,000.", "contents": "The number of parallel fibre-Purkinje dendrite synapses. A morphometric evaluation. Quantitative parameters concerning synapses were studied in the cerebellar molecular layer of 4 cats using ultrastructural morphometric methods. The number of parallel fibre-Purkinje dendrite synapses was estimated to be about 200,000."}
{"id": "PMID:477891", "title": "Influence of presensitization with allogeneic lymphoma cells on the growth and response to therapy of radiation-induced lymphomas in mice.", "content": "Congenic mice were sensitized with viable H-2-incompatible radiation-induced lymphomas (RIL), challenged with syngeneic RIL and treated with bis-chloroethyl-nitrosourea. Either enhancement or inhibition of RIL was found in presensitized mice, depending on the tumor-host system used.", "contents": "Influence of presensitization with allogeneic lymphoma cells on the growth and response to therapy of radiation-induced lymphomas in mice. Congenic mice were sensitized with viable H-2-incompatible radiation-induced lymphomas (RIL), challenged with syngeneic RIL and treated with bis-chloroethyl-nitrosourea. Either enhancement or inhibition of RIL was found in presensitized mice, depending on the tumor-host system used."}
{"id": "PMID:477892", "title": "SEM surface morphology of the contractile cells in the rat seminiferous tubules.", "content": "The SEM observation of the basal surface of the contractile cells in the boundary tissue of the seminiferous tubule of the rat revealed that the contractile cells are extremely flat, vary in shape from rectangular to hexagonal, and are arranged close to each other, in the fashion of a tiled floor, around the seminiferous epithelium.", "contents": "SEM surface morphology of the contractile cells in the rat seminiferous tubules. The SEM observation of the basal surface of the contractile cells in the boundary tissue of the seminiferous tubule of the rat revealed that the contractile cells are extremely flat, vary in shape from rectangular to hexagonal, and are arranged close to each other, in the fashion of a tiled floor, around the seminiferous epithelium."}
{"id": "PMID:477893", "title": "Epithelial cell processes in the development of the secondary palate in the mouse.", "content": "Ultrastructural studies of palatal shelves of Tuck A mice embryos aged 12.25-14.25 days show discontinuities of the epithelial basement membrane traversed by epithelial cell processes before the onset of midline degenerative changes.", "contents": "Epithelial cell processes in the development of the secondary palate in the mouse. Ultrastructural studies of palatal shelves of Tuck A mice embryos aged 12.25-14.25 days show discontinuities of the epithelial basement membrane traversed by epithelial cell processes before the onset of midline degenerative changes."}
{"id": "PMID:477894", "title": "Enhancement of the antitumor effect of illudin S by including it into liposomes.", "content": "Entrapping illudin S in liposome markedly enhanced life prolonging effect in ddN mice bearing Ehrlich ascites tumors, presumably by decreasing the side effects.", "contents": "Enhancement of the antitumor effect of illudin S by including it into liposomes. Entrapping illudin S in liposome markedly enhanced life prolonging effect in ddN mice bearing Ehrlich ascites tumors, presumably by decreasing the side effects."}
{"id": "PMID:477896", "title": "Serum proteins of mice with splenomegaly.", "content": "Our earlier serum electrophoretic study in 'lethargic' mutant mice showed that the quantity of protein in 1 band is inversely related to the size of the spleen. In this study, we demonstrate that this protein band almost entirely disappears in mice with splenomegaly following spontaneous skin infection. The results suggest that this serum protein may play a role in regulating growth of lymphoid tissue.", "contents": "Serum proteins of mice with splenomegaly. Our earlier serum electrophoretic study in 'lethargic' mutant mice showed that the quantity of protein in 1 band is inversely related to the size of the spleen. In this study, we demonstrate that this protein band almost entirely disappears in mice with splenomegaly following spontaneous skin infection. The results suggest that this serum protein may play a role in regulating growth of lymphoid tissue."}
{"id": "PMID:477895", "title": "Estrogen receptor in uteri of mice of different H-2 genotypes.", "content": "A relationship between the amount of available estradiol receptors in uteri of inbred mice and their H-2 genotype is suggested by study in congenic animals.", "contents": "Estrogen receptor in uteri of mice of different H-2 genotypes. A relationship between the amount of available estradiol receptors in uteri of inbred mice and their H-2 genotype is suggested by study in congenic animals."}
{"id": "PMID:477897", "title": "alpha-Mercaptopropionyl-glycine influence on the in vitro proliferative response of human lymphocytes.", "content": "The effect of alpha-mercaptopropinyl-glycine on the in vitro proliferative response of healthy subjects' lymphocytes was studied. Low doses of the drug enhanced spontaneous lymphocyte blastigenesis but had no effect on the PHA-induced blastic response. With increasing concentrations of alpha-mercaptopropionyl-glycine inhibition of 3H-thymidine incorporation was found in unstimulated as well as in PHA stimulated lymphocytes.", "contents": "alpha-Mercaptopropionyl-glycine influence on the in vitro proliferative response of human lymphocytes. The effect of alpha-mercaptopropinyl-glycine on the in vitro proliferative response of healthy subjects' lymphocytes was studied. Low doses of the drug enhanced spontaneous lymphocyte blastigenesis but had no effect on the PHA-induced blastic response. With increasing concentrations of alpha-mercaptopropionyl-glycine inhibition of 3H-thymidine incorporation was found in unstimulated as well as in PHA stimulated lymphocytes."}
{"id": "PMID:477898", "title": "Production of antibodies against bradykinin.", "content": "High-titer antibodies against bradykinin were raised in rabbits. 2 different conjugates of bradykinin were used for immunization: bradykinin coupled to human serum albumin via 1,5-difluoro-2,4-dinitrobenzene and bradykinin coupled to edestin via 1-ethyl-3(3-dimethyl-aminopropyl)-carbodiimide. The sensitivity of the radioimmunoassay method is in the range of 1-50 pg of bradykinin. Cross-reaction of anti-bradykinin antisera occurred with kallidin and met-lys-bradykinin.", "contents": "Production of antibodies against bradykinin. High-titer antibodies against bradykinin were raised in rabbits. 2 different conjugates of bradykinin were used for immunization: bradykinin coupled to human serum albumin via 1,5-difluoro-2,4-dinitrobenzene and bradykinin coupled to edestin via 1-ethyl-3(3-dimethyl-aminopropyl)-carbodiimide. The sensitivity of the radioimmunoassay method is in the range of 1-50 pg of bradykinin. Cross-reaction of anti-bradykinin antisera occurred with kallidin and met-lys-bradykinin."}
{"id": "PMID:477899", "title": "Isolation and electrophoretic identification of exogenous histone from rat blood plasma.", "content": "Histone fractions H1, (H2b+H2a) and H4 were isolated from rat blood plasma and electrophoretically identified 5 min after i.v. exogenous histone application.", "contents": "Isolation and electrophoretic identification of exogenous histone from rat blood plasma. Histone fractions H1, (H2b+H2a) and H4 were isolated from rat blood plasma and electrophoretically identified 5 min after i.v. exogenous histone application."}
{"id": "PMID:477900", "title": "Progesterone receptors in the foetal uterus of guinea-pig: its stimulation after oestradiol treatment.", "content": "This paper shows for the first time the presence of progesterone receptors in the foetal guinea-pig uterus, as well as the stimulation of progesterone receptors in foetal uterus in animals treated with oestradiol.", "contents": "Progesterone receptors in the foetal uterus of guinea-pig: its stimulation after oestradiol treatment. This paper shows for the first time the presence of progesterone receptors in the foetal guinea-pig uterus, as well as the stimulation of progesterone receptors in foetal uterus in animals treated with oestradiol."}
{"id": "PMID:477901", "title": "Radioimmunoassay of plasma progesterone, testosterone, total estrogen and immunoreactive gonadotropin in the nesting and non-nesting green sea turtle, Chelonia mydas (L.).", "content": "Plasma progesterone, testosterone, total estrogens and immunoreactive gonadotropin were measured in nesting and non-nesting sea turtles, Chelonia mydas. Progesterone and gonadotropin concentrations were significantly higher in nesting than in non-nesting turtles, testosterone was not significantly different in either group and total estrogens appeared to be slightly in the nesting group.", "contents": "Radioimmunoassay of plasma progesterone, testosterone, total estrogen and immunoreactive gonadotropin in the nesting and non-nesting green sea turtle, Chelonia mydas (L.). Plasma progesterone, testosterone, total estrogens and immunoreactive gonadotropin were measured in nesting and non-nesting sea turtles, Chelonia mydas. Progesterone and gonadotropin concentrations were significantly higher in nesting than in non-nesting turtles, testosterone was not significantly different in either group and total estrogens appeared to be slightly in the nesting group."}
{"id": "PMID:477902", "title": "Glycogen contents in the rat uterus: response to Hibiscus rosa-sinensis Linn. extracts.", "content": "Ethanolic extracts (50%), as well the benzene extracts, of H. rosa-sinensis Linn. have reduced significantly the glycogen contents in the uterus of adult rat. Both the extracts exhibit a clear-cut dose-response relation. The inhibition in glycogen contents increases as the dose is increased. Of the 2, benzene extract seems to be more potent. The results are due to antiestrogenic nature of the extracts.", "contents": "Glycogen contents in the rat uterus: response to Hibiscus rosa-sinensis Linn. extracts. Ethanolic extracts (50%), as well the benzene extracts, of H. rosa-sinensis Linn. have reduced significantly the glycogen contents in the uterus of adult rat. Both the extracts exhibit a clear-cut dose-response relation. The inhibition in glycogen contents increases as the dose is increased. Of the 2, benzene extract seems to be more potent. The results are due to antiestrogenic nature of the extracts."}
{"id": "PMID:477903", "title": "Effect of lesions of the locus coeruleus complex on the circadian rhythm of plasma corticosterone in the mouse.", "content": "An apparently transient elevation of basal morning (08.00 h) plasma corticosterone levels in male mice was found 48 h after bilateral electrolytic lesions of the brainstem locus coeruleus complex but was not observed 6 weeks after lesioning.", "contents": "Effect of lesions of the locus coeruleus complex on the circadian rhythm of plasma corticosterone in the mouse. An apparently transient elevation of basal morning (08.00 h) plasma corticosterone levels in male mice was found 48 h after bilateral electrolytic lesions of the brainstem locus coeruleus complex but was not observed 6 weeks after lesioning."}
{"id": "PMID:477904", "title": "Monitoring of the dilution rate during continuous in vivo blood sampling with a double lumen catheter.", "content": "A simple and non-destructive method for monitoring the dilution rate during continuous blood sampling is described. When the dilution rate is not constant, the proposed method based on electrical resistivity measurement provides a correction factor for further analysis.", "contents": "Monitoring of the dilution rate during continuous in vivo blood sampling with a double lumen catheter. A simple and non-destructive method for monitoring the dilution rate during continuous blood sampling is described. When the dilution rate is not constant, the proposed method based on electrical resistivity measurement provides a correction factor for further analysis."}
{"id": "PMID:477905", "title": "The structure of amoorastatone and the cytotoxic limonoid 12-hydroxyamoorastatin.", "content": "2 new limonoid-type terpenes have been isolated from an aqueous extract of seeds produced by the Eastern Himalayan (India) plant Aphanamixis grandifolia B1. By interpreting principally mass spectral and nuclear magnetic resonance data, the structures of 12-hydroxyamoorastatin (2b) and amoorastatone (3) were elucidated. Unequivocal evidence for the 12-hydroxyamoorastatin structural assignment was obtained by chemical conversion to sendanin (4). Amoorastatin derivative 2b was found to significantly inhibit growth of the murine P388 lymphocytic leukemia cell lines but amoorastatone in the same system was inactive. In a comparative biological study, sendanin (4) and anthothecol (7) were also found significantly to inhibit growth of the P388 cell line, while rohitukin (8) and limonin (9) were found to be inactive.", "contents": "The structure of amoorastatone and the cytotoxic limonoid 12-hydroxyamoorastatin. 2 new limonoid-type terpenes have been isolated from an aqueous extract of seeds produced by the Eastern Himalayan (India) plant Aphanamixis grandifolia B1. By interpreting principally mass spectral and nuclear magnetic resonance data, the structures of 12-hydroxyamoorastatin (2b) and amoorastatone (3) were elucidated. Unequivocal evidence for the 12-hydroxyamoorastatin structural assignment was obtained by chemical conversion to sendanin (4). Amoorastatin derivative 2b was found to significantly inhibit growth of the murine P388 lymphocytic leukemia cell lines but amoorastatone in the same system was inactive. In a comparative biological study, sendanin (4) and anthothecol (7) were also found significantly to inhibit growth of the P388 cell line, while rohitukin (8) and limonin (9) were found to be inactive."}
{"id": "PMID:477906", "title": "The stimulation of rat brain monoamine oxidase by dietary lithium chloride.", "content": "Lithium chloride administered to rats in drinking water for 30 days caused an increase of whole brain monoamine oxidase specific activity to approximately 140% of control. Carboxylesterase and formyltetrahydrofolate synthetase activities were not affected by Li+ either in vivo or in vitro.", "contents": "The stimulation of rat brain monoamine oxidase by dietary lithium chloride. Lithium chloride administered to rats in drinking water for 30 days caused an increase of whole brain monoamine oxidase specific activity to approximately 140% of control. Carboxylesterase and formyltetrahydrofolate synthetase activities were not affected by Li+ either in vivo or in vitro."}
{"id": "PMID:477907", "title": "The use of dipeptide-p-nitranilides for the study of aminopeptidase specificity.", "content": "The use of dipeptide-p-nitranilides for the study of 2 placental aminopeptidases separated on Sephadex G200 helped in establishing some regular features of their specifities. The high-molecular (320,000 daltons) one prefers Phe in position P'1 to Leu, whereas the lower-molecular aminopeptidase (145,000 daltons) prefers Leu. The high-molecular aminopeptidase splits very slowly the N-terminal Leu when Gly is in adjacent position. Leu-Gly-p-NA is therefore an inhibitor of this AP.", "contents": "The use of dipeptide-p-nitranilides for the study of aminopeptidase specificity. The use of dipeptide-p-nitranilides for the study of 2 placental aminopeptidases separated on Sephadex G200 helped in establishing some regular features of their specifities. The high-molecular (320,000 daltons) one prefers Phe in position P'1 to Leu, whereas the lower-molecular aminopeptidase (145,000 daltons) prefers Leu. The high-molecular aminopeptidase splits very slowly the N-terminal Leu when Gly is in adjacent position. Leu-Gly-p-NA is therefore an inhibitor of this AP."}
{"id": "PMID:477908", "title": "Changes in the activities of protein kinase modulators in the cerebellum of mice due to ethanol, caffeine, or phenobarbital administration.", "content": "Decreased activities of both the inhibitory modulator of adenosine 3':5'-monophosphate (cAMP)-dependent protein kinase (A-PK) as well as the stimulatory modulator of guanosine 3':5'-monophosphate (cGMP)-dependent protein kinase (G-PK) from the mouse cerebellum were noted due to the administration of excessive doses of ethanol, caffeine, and phenobarbital for up to 28 days. The dose-dependent of the inhibition of A-PK or the stimulation of G-PK was observed as a function of the amount of protein kinase modulators in the cerebellum of mice receiving different doses of ethanol.", "contents": "Changes in the activities of protein kinase modulators in the cerebellum of mice due to ethanol, caffeine, or phenobarbital administration. Decreased activities of both the inhibitory modulator of adenosine 3':5'-monophosphate (cAMP)-dependent protein kinase (A-PK) as well as the stimulatory modulator of guanosine 3':5'-monophosphate (cGMP)-dependent protein kinase (G-PK) from the mouse cerebellum were noted due to the administration of excessive doses of ethanol, caffeine, and phenobarbital for up to 28 days. The dose-dependent of the inhibition of A-PK or the stimulation of G-PK was observed as a function of the amount of protein kinase modulators in the cerebellum of mice receiving different doses of ethanol."}
{"id": "PMID:477909", "title": "Acid phosphatase in eggs of the zebrafish, Brachydanio rerio.", "content": "2 isozymes of acid phosphatase have been identified by polyacrylamide disc gel electrophoresis in the ovary and mature, unfertilized eggs of B. rerio. Histochemically, the enzyme appears to be localized in preyolk bodies of previtellogenic oocytes and in yolk platelets of vitellogenic and postvitellogenic oocytes. The contents of the cortical granules at all stages of oocyte differentiation were acid phosphatase negative.", "contents": "Acid phosphatase in eggs of the zebrafish, Brachydanio rerio. 2 isozymes of acid phosphatase have been identified by polyacrylamide disc gel electrophoresis in the ovary and mature, unfertilized eggs of B. rerio. Histochemically, the enzyme appears to be localized in preyolk bodies of previtellogenic oocytes and in yolk platelets of vitellogenic and postvitellogenic oocytes. The contents of the cortical granules at all stages of oocyte differentiation were acid phosphatase negative."}
{"id": "PMID:477910", "title": "Sterilization in the United States.", "content": "Had no U.S. married couples obtained contraceptive sterilizations, there would have been about 800,000 unwanted births during 1971-1973 in addition to the 8.7 million births that actually occurred. More than half of U.S. couples elect sterilization within 10 years after their last wanted birth, and more than six in 10 are sterilized within 15 years.", "contents": "Sterilization in the United States. Had no U.S. married couples obtained contraceptive sterilizations, there would have been about 800,000 unwanted births during 1971-1973 in addition to the 8.7 million births that actually occurred. More than half of U.S. couples elect sterilization within 10 years after their last wanted birth, and more than six in 10 are sterilized within 15 years."}
{"id": "PMID:477914", "title": "Probabilities of intercourse and conception among U.S. teenage women, 1971 and 1976.", "content": "One in five U.S. females have had intercourse by age 16, and two-thirds, by age 19. Almost all the experience is premarital. One in 10 U.S. women get pregnant before age 17; one-quarter before they are 19, and eight in 10 of these pregnancies are premarital. More than one-third of those who are sexually active premaritally have a premarital pregnancy before they turn 19, one-quarter by the time they are 17.", "contents": "Probabilities of intercourse and conception among U.S. teenage women, 1971 and 1976. One in five U.S. females have had intercourse by age 16, and two-thirds, by age 19. Almost all the experience is premarital. One in 10 U.S. women get pregnant before age 17; one-quarter before they are 19, and eight in 10 of these pregnancies are premarital. More than one-third of those who are sexually active premaritally have a premarital pregnancy before they turn 19, one-quarter by the time they are 17."}
{"id": "PMID:477915", "title": "Unintended pregnancies in the United States, 1970--1972.", "content": "There were about 2.0--2.3 million unintended pregnancies in the United States each year during the early 1970s. Within this framework, the rapid rise in the number of abortions since 1973, to 1.3 million in 1977, is quite predictable.", "contents": "Unintended pregnancies in the United States, 1970--1972. There were about 2.0--2.3 million unintended pregnancies in the United States each year during the early 1970s. Within this framework, the rapid rise in the number of abortions since 1973, to 1.3 million in 1977, is quite predictable."}
{"id": "PMID:477912", "title": "Informed consent for fertility control services.", "content": "Informed consent has special relevance for patients seeking fertility control services who are not ill in the usual sense. Genuine informed consent requirements should not be confused with spurious ones induced by political pressure.", "contents": "Informed consent for fertility control services. Informed consent has special relevance for patients seeking fertility control services who are not ill in the usual sense. Genuine informed consent requirements should not be confused with spurious ones induced by political pressure."}
{"id": "PMID:477924", "title": "The risk of adolescent pregnancy in the first months of intercourse.", "content": "Because of early age at initiation of intercourse and associated nonuse of contraception, half of first premarital pregnancies to teens occur in the first six months after they begin coitus; one-fifth occur in the first month. Programs to prevent adolescent pregnancy will not succeed unless they reach young people before they begin sexual activity.", "contents": "The risk of adolescent pregnancy in the first months of intercourse. Because of early age at initiation of intercourse and associated nonuse of contraception, half of first premarital pregnancies to teens occur in the first six months after they begin coitus; one-fifth occur in the first month. Programs to prevent adolescent pregnancy will not succeed unless they reach young people before they begin sexual activity."}
{"id": "PMID:477913", "title": "Warning: cigarette smoking is dangerous to reproductive health.", "content": "Family planning clinics have a special responsibility to educate patients about smoking hazards, since pill users, young mothers and mothers-to-be run high health risks if they smoke. The clinics agree, but their educational efforts vary.", "contents": "Warning: cigarette smoking is dangerous to reproductive health. Family planning clinics have a special responsibility to educate patients about smoking hazards, since pill users, young mothers and mothers-to-be run high health risks if they smoke. The clinics agree, but their educational efforts vary."}
{"id": "PMID:477926", "title": "Family planning visits to private physicians.", "content": "The NAMCS data indicate that women aged 15--44 made an estimated 9.7 million visits to office-based physicians in 1977 during which they obtained medical family planning services, examinations or counseling. Two hundred such visits were made per 1,000 women of reproductive age during the year. The majority of these visits were by women aged 20--34. Family planning services were included in five percent of all office visits made by women of reproductive age. While the rate of family planning visits to physicians' offices varied by race, geographical area and urban/rural setting, these variations reflected the distribution of visits for all purposes, and not the distribution of family planning visits in particular. According to the NAMCS, men aged 20--54 made an estimated 791,000 family planning visits to office-based physicians in 1977. This indicates that there were about 17 office family planning visits per 1,000 men in this age-group, or about 12 visits by women for every one by men.", "contents": "Family planning visits to private physicians. The NAMCS data indicate that women aged 15--44 made an estimated 9.7 million visits to office-based physicians in 1977 during which they obtained medical family planning services, examinations or counseling. Two hundred such visits were made per 1,000 women of reproductive age during the year. The majority of these visits were by women aged 20--34. Family planning services were included in five percent of all office visits made by women of reproductive age. While the rate of family planning visits to physicians' offices varied by race, geographical area and urban/rural setting, these variations reflected the distribution of visits for all purposes, and not the distribution of family planning visits in particular. According to the NAMCS, men aged 20--54 made an estimated 791,000 family planning visits to office-based physicians in 1977. This indicates that there were about 17 office family planning visits per 1,000 men in this age-group, or about 12 visits by women for every one by men."}
{"id": "PMID:477925", "title": "Community determinants of U.S. legal abortion rates.", "content": "The availability of abortion services--the number of facilities and the number of types of agencies providing abortions--is the most powerful determinant of variations in abortion rates in U.S. metropolitan communities. The increase in abortion availability is also the most important factor in explaining the increase in abortion rates that occurred between 1973 and 1975. Other factors affecting abortion rates (but less substantially) include rural residence, population density, female labor force participation, the proportion Catholic and the proportion receiving public assistance.", "contents": "Community determinants of U.S. legal abortion rates. The availability of abortion services--the number of facilities and the number of types of agencies providing abortions--is the most powerful determinant of variations in abortion rates in U.S. metropolitan communities. The increase in abortion availability is also the most important factor in explaining the increase in abortion rates that occurred between 1973 and 1975. Other factors affecting abortion rates (but less substantially) include rural residence, population density, female labor force participation, the proportion Catholic and the proportion receiving public assistance."}
{"id": "PMID:477927", "title": "Barrier methods: renewed interest, but more research needed.", "content": "At a recent major international workshop held in Guatemala, the state of the art of barrier contraception was examined. While there are promising leads to new methods, none is likely to be available in the near future.", "contents": "Barrier methods: renewed interest, but more research needed. At a recent major international workshop held in Guatemala, the state of the art of barrier contraception was examined. While there are promising leads to new methods, none is likely to be available in the near future."}
{"id": "PMID:477955", "title": "[Characteristics of the effect of antorphine on biochemical processes].", "content": "It has been shown in experiments on rats that the effect of antorphin (0.57 mg/kg), morphine (1 mg/kg) and promedol (2 mg/kg) on catecholamine balance and phosphorylase activity correlates with changes in the carbohydrate metabolism of tissues. The action of antorphin as compared to that of morphine and promedol is associated with a decreased content of adrenaline and preservation of glycogen depot in the tissues of the heart, liver and skeletal muscles.", "contents": "[Characteristics of the effect of antorphine on biochemical processes]. It has been shown in experiments on rats that the effect of antorphin (0.57 mg/kg), morphine (1 mg/kg) and promedol (2 mg/kg) on catecholamine balance and phosphorylase activity correlates with changes in the carbohydrate metabolism of tissues. The action of antorphin as compared to that of morphine and promedol is associated with a decreased content of adrenaline and preservation of glycogen depot in the tissues of the heart, liver and skeletal muscles."}
{"id": "PMID:477956", "title": "[Effect of nonachlazine on cerebral circulation].", "content": "It has been shown in acute experiments on anesthetized cats that nonachlazine in a dose of 2.5 mg/kg produces a short-term decrease followed by more prolonged elevation of the tone of cerebral vessels, lower extremities and systemic arterial blood pressure. Previous blocking of alpha- and beta-adrenoreceptors decreases or abolishes the development of the above effects. In a dose of 5 mg/kg nonachlazine produces no rise in the tone of intracranial vessels, but increases local and common cerebral blood flow. An increase in PO2 in the brain cortex correlates well with a rise in the blood flow.", "contents": "[Effect of nonachlazine on cerebral circulation]. It has been shown in acute experiments on anesthetized cats that nonachlazine in a dose of 2.5 mg/kg produces a short-term decrease followed by more prolonged elevation of the tone of cerebral vessels, lower extremities and systemic arterial blood pressure. Previous blocking of alpha- and beta-adrenoreceptors decreases or abolishes the development of the above effects. In a dose of 5 mg/kg nonachlazine produces no rise in the tone of intracranial vessels, but increases local and common cerebral blood flow. An increase in PO2 in the brain cortex correlates well with a rise in the blood flow."}
{"id": "PMID:477954", "title": "[Aminazine distribution in the organs and tissues of rats depending on the routes of administration and the age of the animals].", "content": "Distribution of intramuscular and oral aminazin (20 mg/kg) in the brain, lungs, liver, kidneys, heart, ileum, blood and urine was studied in young and old male rats. Intramuscular drug showed maximal accumulation in the lungs and intestine, respectively, whereas at oral administration maximal and minimal accumulation was recorded in the liver and kidneys. Urine excretion of parenteral aminazin was 4-7-fold higher as compared to oral administration. Delayed accumulation of aminazin in the organs and its decreased excretion with urine were recorded in old animals.", "contents": "[Aminazine distribution in the organs and tissues of rats depending on the routes of administration and the age of the animals]. Distribution of intramuscular and oral aminazin (20 mg/kg) in the brain, lungs, liver, kidneys, heart, ileum, blood and urine was studied in young and old male rats. Intramuscular drug showed maximal accumulation in the lungs and intestine, respectively, whereas at oral administration maximal and minimal accumulation was recorded in the liver and kidneys. Urine excretion of parenteral aminazin was 4-7-fold higher as compared to oral administration. Delayed accumulation of aminazin in the organs and its decreased excretion with urine were recorded in old animals."}
{"id": "PMID:477957", "title": "[Effect of agents used in the therapy of acute ischemic heart disease on the histomorphological changes in the myocardium in acute dystrophy].", "content": "The morphological analysis of the portions of the interventricular septum and left ventricular wall in cats has revealed dystrophic and necrotic changes in the myocardium after administration of caffeine followed by adrenaline: haemorrhages, intravascular thrombogenesis, dystrophic and necrobiotic changes in myocytes. In early periods of acute ischemic heart disease morphine, fibrinolysin, heparin and Inosie F either abolished (fibrinolysin) or considerably decreased (heparin and Inosie F) a tendency toward intravascular thrombogenesis and damage to the myocardial parenchyma.", "contents": "[Effect of agents used in the therapy of acute ischemic heart disease on the histomorphological changes in the myocardium in acute dystrophy]. The morphological analysis of the portions of the interventricular septum and left ventricular wall in cats has revealed dystrophic and necrotic changes in the myocardium after administration of caffeine followed by adrenaline: haemorrhages, intravascular thrombogenesis, dystrophic and necrobiotic changes in myocytes. In early periods of acute ischemic heart disease morphine, fibrinolysin, heparin and Inosie F either abolished (fibrinolysin) or considerably decreased (heparin and Inosie F) a tendency toward intravascular thrombogenesis and damage to the myocardial parenchyma."}
{"id": "PMID:477959", "title": "[Dynamics of bilignost distribution in different exposures of the body in white rats].", "content": "The dynamics of distribution of 125I- and 131I-bilignost in intact rats has been studied in experimental pathological condition of the liver. It has been shown that the cholecystographic radiographic contrast agent (RCA) bilignost is absorbed and accumulated mainly by the liver. This indicates that the preparation elicits a selective action with respect to the liver. Administration of 125I-bilignost in conjunction with the unlabeled cholecystographic agents bilignost and endografin induces competition for absorption by the liver, which distinguishes these preparations from the urographic agent cardiotrast decreasing accumulation of the label by the kidneys. Acute CCl4 poisoning of the animals results in a sharp decrease in the liver capacity for accumulation of 131I-bilignost and to a slight rise in the preparation kidney level. Three-day administration of phenobarbital to rats produces a considerable drop in 125I-bilignost level in all the organs tested, suggesting an enhanced RCA excretion from the body.", "contents": "[Dynamics of bilignost distribution in different exposures of the body in white rats]. The dynamics of distribution of 125I- and 131I-bilignost in intact rats has been studied in experimental pathological condition of the liver. It has been shown that the cholecystographic radiographic contrast agent (RCA) bilignost is absorbed and accumulated mainly by the liver. This indicates that the preparation elicits a selective action with respect to the liver. Administration of 125I-bilignost in conjunction with the unlabeled cholecystographic agents bilignost and endografin induces competition for absorption by the liver, which distinguishes these preparations from the urographic agent cardiotrast decreasing accumulation of the label by the kidneys. Acute CCl4 poisoning of the animals results in a sharp decrease in the liver capacity for accumulation of 131I-bilignost and to a slight rise in the preparation kidney level. Three-day administration of phenobarbital to rats produces a considerable drop in 125I-bilignost level in all the organs tested, suggesting an enhanced RCA excretion from the body."}
{"id": "PMID:477968", "title": "QSAR of alkyl pyridinecarbonyldithiocarbazates as uncouplers of oxidative phosphorylation.", "content": "The structure-activity relationship for alkyl dithiocarbazates and alkyl pyridinecarbonyldithiocarbazates as oncouplers of oxidative phosphorylation in rat liver mitochondria has been studied using subsituent constants and regression analysis. As in various other uncouplers, the hydrophobic property of substituents gave a good correlation with their biological activities. 4-Pyridinecarbonyldithiocarbazates are the most active among the congeners studied.", "contents": "QSAR of alkyl pyridinecarbonyldithiocarbazates as uncouplers of oxidative phosphorylation. The structure-activity relationship for alkyl dithiocarbazates and alkyl pyridinecarbonyldithiocarbazates as oncouplers of oxidative phosphorylation in rat liver mitochondria has been studied using subsituent constants and regression analysis. As in various other uncouplers, the hydrophobic property of substituents gave a good correlation with their biological activities. 4-Pyridinecarbonyldithiocarbazates are the most active among the congeners studied."}
{"id": "PMID:477969", "title": "[Chemical and pharmacological research on pyran derivatives. XIV. 3-alkylaminoaphtho/2,1-b/pyran-1-ones and derivatives].", "content": "3-Alkyl(phenyl)aminoaphtho[2,1-b]pyran-1-ones (III) were prepared from N-alkyl or N-phenylethoxycarbonylacetamides and 2-naphthol in the presence of phosphorus oxychloride, in order to evaluate their pharmacological activity on the CNS in comparison with previously described 3-dialkylaminoaphtho[2,1-b]pyran-1-ones. Compounds (III) gave 2-morpholinomethyl derivatives as well as N-acetyl and N-ethtoxycarbonyl derivatives. The reaction of (III) in which R = alkyl and N,N-dimethylformamide-POCl3 afforded 2-formyl derivatives and in some cases also 8-alkyl-9,10-bisdimethylaminoaphtho[1',2':5,6]pyrano[2,3-b]pyrrol-11(8H)-ones; when R = phenyl, only naphtho[1',2':5,6]pyrano[2,3-b]quinolin-14-one was obtained from the same reaction. Pharmacological evaluation showed that compounds (III) had a weak CNS depressant activity. Some of them also exhibited antagonist effect on reserpine-induced blepharospasm and hypothermia and on metrazole-induced seizures in the mouse. Within the limits of these activities a special behavior was found for the compound 3-ethylaminoaphtho[2,1-b]pyran-1-one [(III b) - K 12479].", "contents": "[Chemical and pharmacological research on pyran derivatives. XIV. 3-alkylaminoaphtho/2,1-b/pyran-1-ones and derivatives]. 3-Alkyl(phenyl)aminoaphtho[2,1-b]pyran-1-ones (III) were prepared from N-alkyl or N-phenylethoxycarbonylacetamides and 2-naphthol in the presence of phosphorus oxychloride, in order to evaluate their pharmacological activity on the CNS in comparison with previously described 3-dialkylaminoaphtho[2,1-b]pyran-1-ones. Compounds (III) gave 2-morpholinomethyl derivatives as well as N-acetyl and N-ethtoxycarbonyl derivatives. The reaction of (III) in which R = alkyl and N,N-dimethylformamide-POCl3 afforded 2-formyl derivatives and in some cases also 8-alkyl-9,10-bisdimethylaminoaphtho[1',2':5,6]pyrano[2,3-b]pyrrol-11(8H)-ones; when R = phenyl, only naphtho[1',2':5,6]pyrano[2,3-b]quinolin-14-one was obtained from the same reaction. Pharmacological evaluation showed that compounds (III) had a weak CNS depressant activity. Some of them also exhibited antagonist effect on reserpine-induced blepharospasm and hypothermia and on metrazole-induced seizures in the mouse. Within the limits of these activities a special behavior was found for the compound 3-ethylaminoaphtho[2,1-b]pyran-1-one [(III b) - K 12479]."}
{"id": "PMID:477963", "title": "[Comparative study of the effect of decamethoxine, decamine and levorin on carbohydrate metabolic indices in the liver of white rats].", "content": "Intramuscular injection of decamin into the animals in a dose of 0.5 and 1 mg/kg has no significant effect on carbohydrate metabolism in the liver of white rats. Decamethoxin and levorin injected in the same doses, specifically in a dose of 1 mg/kg, reduced the level of glucose as well as that of total and free glycogen in the liver. The drugs lowered also the activity of phosphorylase and glocoso-6-phosphatase. Meanwhile the activity of hexokinase, lactate dehydrogenase and phosphoglucosiomerase was potentiated. The animals given decamethoxin showed the aforesaid parameters returning to normal 20 days after the drug was discontinued, whereas similar changes were not found in the rats on levorin.", "contents": "[Comparative study of the effect of decamethoxine, decamine and levorin on carbohydrate metabolic indices in the liver of white rats]. Intramuscular injection of decamin into the animals in a dose of 0.5 and 1 mg/kg has no significant effect on carbohydrate metabolism in the liver of white rats. Decamethoxin and levorin injected in the same doses, specifically in a dose of 1 mg/kg, reduced the level of glucose as well as that of total and free glycogen in the liver. The drugs lowered also the activity of phosphorylase and glocoso-6-phosphatase. Meanwhile the activity of hexokinase, lactate dehydrogenase and phosphoglucosiomerase was potentiated. The animals given decamethoxin showed the aforesaid parameters returning to normal 20 days after the drug was discontinued, whereas similar changes were not found in the rats on levorin."}
{"id": "PMID:477962", "title": "[Pharmacological properties of some vinyl derivatives of quinoline].", "content": "Toxicity, radioprotective and antiinflammatory properties of 20 vinyl derivatives of quinoline and products of their transformations were studied in experiments on Wistar mice and rats. The salts of quinoline vinyl derivatives (compounds VI, IX, XII, and XVIII) were shown to be more toxic than respective initial compounds (VI, VIII, XI and XVI). 8-(beta-Butylthio)-ethyloxyquinoline (compound IV) protected 25% of irradiated mice from death. Compounds I, XII, XVI and XVIII exerted a radioprotective action (in 10-15%), whereas the remaining substances proved ineffective. The combination of 8-vinyloxyquinoline and iodine (compound IV) elicited a marked antiinflammatory action in experimental serotonin-, dextran- and to a less measure formalin-induced inflammation.", "contents": "[Pharmacological properties of some vinyl derivatives of quinoline]. Toxicity, radioprotective and antiinflammatory properties of 20 vinyl derivatives of quinoline and products of their transformations were studied in experiments on Wistar mice and rats. The salts of quinoline vinyl derivatives (compounds VI, IX, XII, and XVIII) were shown to be more toxic than respective initial compounds (VI, VIII, XI and XVI). 8-(beta-Butylthio)-ethyloxyquinoline (compound IV) protected 25% of irradiated mice from death. Compounds I, XII, XVI and XVIII exerted a radioprotective action (in 10-15%), whereas the remaining substances proved ineffective. The combination of 8-vinyloxyquinoline and iodine (compound IV) elicited a marked antiinflammatory action in experimental serotonin-, dextran- and to a less measure formalin-induced inflammation."}
{"id": "PMID:477961", "title": "[Antianemic effect of ampevit and orkomin].", "content": "The antianemic action of ampevit and orkomin developed at the Laboratory of Experimental Therapy, Institute of Gerontology, Academy of Medical Sciences of the USSR was studied in experiments on young and old animals. It has been established that repeated administration of the drugs increases the reticulocyte count in both groups of animals. Ampevit diminishes severity of posthemorrhagic anemia. Both drugs promote more rapid recovery of hemoglobin content and red cell count in old animals after blood loss. The data obtained were supported by clinical trials of the drugs which have been recommended for combined treatment of posthemorrhagic anemias and other conditions accompanied by weakened function of the growing red fiber of the bone marrow seen also in people of old and senile age.", "contents": "[Antianemic effect of ampevit and orkomin]. The antianemic action of ampevit and orkomin developed at the Laboratory of Experimental Therapy, Institute of Gerontology, Academy of Medical Sciences of the USSR was studied in experiments on young and old animals. It has been established that repeated administration of the drugs increases the reticulocyte count in both groups of animals. Ampevit diminishes severity of posthemorrhagic anemia. Both drugs promote more rapid recovery of hemoglobin content and red cell count in old animals after blood loss. The data obtained were supported by clinical trials of the drugs which have been recommended for combined treatment of posthemorrhagic anemias and other conditions accompanied by weakened function of the growing red fiber of the bone marrow seen also in people of old and senile age."}
{"id": "PMID:477958", "title": "[Effect of ascorbic acid and rutin on the development of experimental peroxide atherosclerosis].", "content": "It has been established in experiments on 73 rabbits that ascorbic acid inhibits the development of hypercholesterinemia, peroxidation syndrome and atherosclerotic affection of the aortas. Rutin delays the development of hypercholesterinemia and peroxidation syndrome and tends to inhibiting aortal atherosclerotic affection.", "contents": "[Effect of ascorbic acid and rutin on the development of experimental peroxide atherosclerosis]. It has been established in experiments on 73 rabbits that ascorbic acid inhibits the development of hypercholesterinemia, peroxidation syndrome and atherosclerotic affection of the aortas. Rutin delays the development of hypercholesterinemia and peroxidation syndrome and tends to inhibiting aortal atherosclerotic affection."}
{"id": "PMID:477964", "title": "[Pharmacokinetics of the antiviral preparation, bonafton].", "content": "The parameters of bonafton pharmacokinetics (absorption, distribution, excretion) have been studied in male Wistar rats at single enteral and intraperitoneal administration. With these routes of administration daily resorption amounts to 26 and 100%, respectively. Regardless administration routes 14C-bonafton distributes in a similar way in the internal organs and tissues and is excreted through the kidneys and gastrointestinal tract, the latter way of excretion being predominant. T1 = 0.25 day (24%) and T2 = 1.8 day. A two-chamber model of the drug metabolism is suggested. This model allows a calculation of bonafton and its metabolites accumulation at repeated administration of the drug to the body.", "contents": "[Pharmacokinetics of the antiviral preparation, bonafton]. The parameters of bonafton pharmacokinetics (absorption, distribution, excretion) have been studied in male Wistar rats at single enteral and intraperitoneal administration. With these routes of administration daily resorption amounts to 26 and 100%, respectively. Regardless administration routes 14C-bonafton distributes in a similar way in the internal organs and tissues and is excreted through the kidneys and gastrointestinal tract, the latter way of excretion being predominant. T1 = 0.25 day (24%) and T2 = 1.8 day. A two-chamber model of the drug metabolism is suggested. This model allows a calculation of bonafton and its metabolites accumulation at repeated administration of the drug to the body."}
{"id": "PMID:477960", "title": "[Effect of histamine on blood and lymph coagulability].", "content": "It has been established in dog experiments that histamine (0.05 mg/kg) induces a considerable rise in blood coagulation, an increase in prothrombin consumption and plasma tolerance to heparin a diminution of fibrinase content and enhancement of fibrinolysis. Lymph coagulation and fibrinolytic activity remain unchanged.", "contents": "[Effect of histamine on blood and lymph coagulability]. It has been established in dog experiments that histamine (0.05 mg/kg) induces a considerable rise in blood coagulation, an increase in prothrombin consumption and plasma tolerance to heparin a diminution of fibrinase content and enhancement of fibrinolysis. Lymph coagulation and fibrinolytic activity remain unchanged."}
{"id": "PMID:477965", "title": "[Gonadotoxic action of acetylsalicylic acid].", "content": "It has been established in experiments on white rats that administration of acetylsalicylic acid (ASA) in a dose of 1/10 LD50 for 1 1/2 months is accompanied by a decrease in the functional activity of spermatozoids. Repeated inhalation of ASA dust at a concentration of 25 mg/m3 for 4 months produces morphological changes in the spermatogenic epithelium and abnormal antenatal development of the progeny of male animals under test.", "contents": "[Gonadotoxic action of acetylsalicylic acid]. It has been established in experiments on white rats that administration of acetylsalicylic acid (ASA) in a dose of 1/10 LD50 for 1 1/2 months is accompanied by a decrease in the functional activity of spermatozoids. Repeated inhalation of ASA dust at a concentration of 25 mg/m3 for 4 months produces morphological changes in the spermatogenic epithelium and abnormal antenatal development of the progeny of male animals under test."}
{"id": "PMID:477966", "title": "[Protective action of estrogen-progestin preparations in reproductive function disorder from subtoxic doses of organophosphorus compounds].", "content": "The action of subtoxic doses of armine and nibufin administered alone and combined with steroid drugs on the rat reproductive function and uterine tissue RNA content was studied experimentally. The subtoxic doses of the drugs increased sharply the luteinizing hormone releasing factor content in the hypothalamus, as well as the synthesis and excretion of luteinizing and follicle-stimulating hormones and RNA content in the uterine tissue. Estrogen-progestin drugs, particularly infecundin, exerted a protective action in armine- and nibufin-produced abnormalities of the reproductive function.", "contents": "[Protective action of estrogen-progestin preparations in reproductive function disorder from subtoxic doses of organophosphorus compounds]. The action of subtoxic doses of armine and nibufin administered alone and combined with steroid drugs on the rat reproductive function and uterine tissue RNA content was studied experimentally. The subtoxic doses of the drugs increased sharply the luteinizing hormone releasing factor content in the hypothalamus, as well as the synthesis and excretion of luteinizing and follicle-stimulating hormones and RNA content in the uterine tissue. Estrogen-progestin drugs, particularly infecundin, exerted a protective action in armine- and nibufin-produced abnormalities of the reproductive function."}
{"id": "PMID:478013", "title": "Recognition of antigenic differences among neurons using antiserums to clonal neural retina hybrid cells.", "content": "Antiserums prepared against hybrid cell strains formed between freshly isolated rodent neural retina cells and a human fibroblast cell line W.I. 18, VA-2 recognize cell surface antigens that are 1) restricted to the neural retina, 2) present in both embryonic and adult retina, and 3) localized to groups of cells within the retina. Normal segregants (i.e., those lines that had retained rodent chromosomes and lost human chromosomes) were used as immunogens in rabbits to produce the antiserums. Antiserums against both whole cells and purified plasma membranes were adsorbed with human parent VA-2 cells and nonneural and neural rodent tissue to remove cross-reacting specificities. All six antiserums studied continued to react with embryonic retina after brain cross-reactivity was removed.", "contents": "Recognition of antigenic differences among neurons using antiserums to clonal neural retina hybrid cells. Antiserums prepared against hybrid cell strains formed between freshly isolated rodent neural retina cells and a human fibroblast cell line W.I. 18, VA-2 recognize cell surface antigens that are 1) restricted to the neural retina, 2) present in both embryonic and adult retina, and 3) localized to groups of cells within the retina. Normal segregants (i.e., those lines that had retained rodent chromosomes and lost human chromosomes) were used as immunogens in rabbits to produce the antiserums. Antiserums against both whole cells and purified plasma membranes were adsorbed with human parent VA-2 cells and nonneural and neural rodent tissue to remove cross-reacting specificities. All six antiserums studied continued to react with embryonic retina after brain cross-reactivity was removed."}
{"id": "PMID:478014", "title": "The role of acetylcholine receptor antibodies in myasthenia gravis.", "content": "Myasthenia gravis is an autoimmune disease of man characterized by remitting and relapsing muscle fatigability. Although the etiology and pathogenesis are incompletely understood, the presence of circulating antibodies directed against the nicotinic acetylcholine (ACh) receptor in 80--90% of patients with myasthenia gravis and the identification of immune complexes at their neuromuscular junction have helped explain the altered neuromuscular transmission. The ACh receptor antibodies do not block access of ACh to the receptor, but do decrease the number of receptors by accelerating their degradation both in rat myotube cultures and in vivo models. In vitro these antibodies play a major role in myasthenia gravis. However, correlations of antibody titers with the clinical state following thymectomy or in neonatal myasthenia suggest that host factors may be equally important in determining whether the ACh receptor antibodies will result in clinical myasthenia.", "contents": "The role of acetylcholine receptor antibodies in myasthenia gravis. Myasthenia gravis is an autoimmune disease of man characterized by remitting and relapsing muscle fatigability. Although the etiology and pathogenesis are incompletely understood, the presence of circulating antibodies directed against the nicotinic acetylcholine (ACh) receptor in 80--90% of patients with myasthenia gravis and the identification of immune complexes at their neuromuscular junction have helped explain the altered neuromuscular transmission. The ACh receptor antibodies do not block access of ACh to the receptor, but do decrease the number of receptors by accelerating their degradation both in rat myotube cultures and in vivo models. In vitro these antibodies play a major role in myasthenia gravis. However, correlations of antibody titers with the clinical state following thymectomy or in neonatal myasthenia suggest that host factors may be equally important in determining whether the ACh receptor antibodies will result in clinical myasthenia."}
{"id": "PMID:478017", "title": "[So-called constancy of chyme composition].", "content": "Study of the chyme composition in different phases of digestion at different levels of the small intestine showed the composition to be stabilized enough in respect to the water and electrolytes contents as well as the main nutricious substances. Gradual increase in concentration of the main NSs occurs in the advancing chyme. The data obtained suggest that the constancy of the chyme composition is only valid in respect to the water and electrolytes regardless of their unequal contents in the food.", "contents": "[So-called constancy of chyme composition]. Study of the chyme composition in different phases of digestion at different levels of the small intestine showed the composition to be stabilized enough in respect to the water and electrolytes contents as well as the main nutricious substances. Gradual increase in concentration of the main NSs occurs in the advancing chyme. The data obtained suggest that the constancy of the chyme composition is only valid in respect to the water and electrolytes regardless of their unequal contents in the food."}
{"id": "PMID:478019", "title": "[Dynamics of several indices of the enzyme-hormone status of the body after hypobaria and during decompression sickness].", "content": "Hormone content and enzyme activity change under the influence of hyperbaria and in decompression disease long before the symptoms of the disease appear. Apart from that, an increase in adrenalin, noradrenalin, 17-oxycorticosteroids, and glucose concentrations, erythrocyte number and hemoglobin, as well as lowering of amylase and catalase activities are the indicative signs for prognostication and diagnosis of the decompression disease.", "contents": "[Dynamics of several indices of the enzyme-hormone status of the body after hypobaria and during decompression sickness]. Hormone content and enzyme activity change under the influence of hyperbaria and in decompression disease long before the symptoms of the disease appear. Apart from that, an increase in adrenalin, noradrenalin, 17-oxycorticosteroids, and glucose concentrations, erythrocyte number and hemoglobin, as well as lowering of amylase and catalase activities are the indicative signs for prognostication and diagnosis of the decompression disease."}
{"id": "PMID:478020", "title": "[Effect of potassium ions on the contractile activity of renal artery smooth muscle].", "content": "Study of isolated segments of renal arteries in rabbits showed that decrease of potassium ion concentration in the bathing fluid was followed by increase in tension, while its increase from 5 meq/l to 10 meq/l was accompanied by gradual relaxation of vessel muscles and increase of their sensitivity to noradrenalin (NA). This relationship was lacking in segments activated with NA. The ability of NA and angiotensin to activate renal arterial muscles by electromechanic and pharmacomechanic coupling mechanismes was proved experimentally. The paper discussed the role of the cell membrane sodium potassium pump in vascular muscles.", "contents": "[Effect of potassium ions on the contractile activity of renal artery smooth muscle]. Study of isolated segments of renal arteries in rabbits showed that decrease of potassium ion concentration in the bathing fluid was followed by increase in tension, while its increase from 5 meq/l to 10 meq/l was accompanied by gradual relaxation of vessel muscles and increase of their sensitivity to noradrenalin (NA). This relationship was lacking in segments activated with NA. The ability of NA and angiotensin to activate renal arterial muscles by electromechanic and pharmacomechanic coupling mechanismes was proved experimentally. The paper discussed the role of the cell membrane sodium potassium pump in vascular muscles."}
{"id": "PMID:478021", "title": "[Renal potassium excretion in rats exposed to different carbohydrate metabolism treatments].", "content": "After administration of 5 ml/100 g of 1.25% KCl solution into the rat stomach, the excretion of potassium in urine was 529.7 +/- 19.4 mueq/100 g.4 hours. After the same amount of potassium in 1--10% solutions, 2% solution of fructose, or 2% solution of mannitol, the potassium excretion reduced by over 25%. Glucose exerted no effect on the sodium excretion. Injection of 0.4 unit of insulin per 100 g of body weight along with administration of standard amount of potassium led to a decrease in the potassium excretion to 358.8 +/- 16.8 mueq/100 g.4 hours. The data obtained suggest a close relation between the carbohydrate metabolism and the potassium transport in the kidneys.", "contents": "[Renal potassium excretion in rats exposed to different carbohydrate metabolism treatments]. After administration of 5 ml/100 g of 1.25% KCl solution into the rat stomach, the excretion of potassium in urine was 529.7 +/- 19.4 mueq/100 g.4 hours. After the same amount of potassium in 1--10% solutions, 2% solution of fructose, or 2% solution of mannitol, the potassium excretion reduced by over 25%. Glucose exerted no effect on the sodium excretion. Injection of 0.4 unit of insulin per 100 g of body weight along with administration of standard amount of potassium led to a decrease in the potassium excretion to 358.8 +/- 16.8 mueq/100 g.4 hours. The data obtained suggest a close relation between the carbohydrate metabolism and the potassium transport in the kidneys."}
{"id": "PMID:478026", "title": "[Concentration of water and electrolytes in rat liver during absorption of salt solutions].", "content": "Administration of water and 0.9% NaCl; 1.25% KCl; 0.75% MgCl2 solutions (5 ml/100 g body weight) into the rat stomach revealed that large amount of water was not detained in the liver but just passed through it. The electrolytes contents did not change significantly in the liver. Administration of KCl and MgCl2 solutions increased concentration of respective cations in the blood. The data obtained suggest that water and salts absorbed in the small intestine pass through the liver into the general blood flow and are detained in different tissues.", "contents": "[Concentration of water and electrolytes in rat liver during absorption of salt solutions]. Administration of water and 0.9% NaCl; 1.25% KCl; 0.75% MgCl2 solutions (5 ml/100 g body weight) into the rat stomach revealed that large amount of water was not detained in the liver but just passed through it. The electrolytes contents did not change significantly in the liver. Administration of KCl and MgCl2 solutions increased concentration of respective cations in the blood. The data obtained suggest that water and salts absorbed in the small intestine pass through the liver into the general blood flow and are detained in different tissues."}
{"id": "PMID:478030", "title": "[Secretion of neurohypophyseal hormones following partial exclusion of peripheral reflex connections].", "content": "Influence of complete transversal section of spinal cord on secretion of hormones by hypothalamo-hypophyseal system under adaptive restoration of hyrosecretory function of kidneys was studied in chronic experiments on 28 dogs. In separate terms after the operation the level of antidiuretic hormones and oxytocin in blood plasma did not correspond to degree of organism's hydration--in contrast to intact animals. The mechanism of reciprocal secretion of hormones into the blood and CSF after the spinal section became disordered. The blood--brain barrier remained impenetrable for hormones of neurohypophysis in conditions of the spinal trauma.", "contents": "[Secretion of neurohypophyseal hormones following partial exclusion of peripheral reflex connections]. Influence of complete transversal section of spinal cord on secretion of hormones by hypothalamo-hypophyseal system under adaptive restoration of hyrosecretory function of kidneys was studied in chronic experiments on 28 dogs. In separate terms after the operation the level of antidiuretic hormones and oxytocin in blood plasma did not correspond to degree of organism's hydration--in contrast to intact animals. The mechanism of reciprocal secretion of hormones into the blood and CSF after the spinal section became disordered. The blood--brain barrier remained impenetrable for hormones of neurohypophysis in conditions of the spinal trauma."}
{"id": "PMID:478032", "title": "[Correlation between cholesterol and lecithin in the blood and muscles and physiologic indices in rats developing in various stress states].", "content": "Development female rats during the 1st year of life, under conditions of dynamic exercises caused starting from the 4th month, a decrease in the blood cholesterin and the raise of lecithin and lecithin--cholesterin index (L/Ch). No hypercholesterinemia common for the control animals develops by the 13th month in these animals. The cholesterin content increases in their muscles. Static loads launch less beneficent shifts of the above parameters. These rats reveal larger amount of the blood cholesterin along with its decrease in the muscles by the end of the 1st year. Under nociceptive effects the rats develop hypercholesterinemia and hypolecithinemia yet by the 7th month which accounts for a sharply reduced L/Ch index. At 13 months cholesterin is obviously decreased in the muscles of these rats. Resting energy expenditure is increased in these rats due to activated thyroid function and to a decrease in the cholinergic maintenance of regulation.", "contents": "[Correlation between cholesterol and lecithin in the blood and muscles and physiologic indices in rats developing in various stress states]. Development female rats during the 1st year of life, under conditions of dynamic exercises caused starting from the 4th month, a decrease in the blood cholesterin and the raise of lecithin and lecithin--cholesterin index (L/Ch). No hypercholesterinemia common for the control animals develops by the 13th month in these animals. The cholesterin content increases in their muscles. Static loads launch less beneficent shifts of the above parameters. These rats reveal larger amount of the blood cholesterin along with its decrease in the muscles by the end of the 1st year. Under nociceptive effects the rats develop hypercholesterinemia and hypolecithinemia yet by the 7th month which accounts for a sharply reduced L/Ch index. At 13 months cholesterin is obviously decreased in the muscles of these rats. Resting energy expenditure is increased in these rats due to activated thyroid function and to a decrease in the cholinergic maintenance of regulation."}
{"id": "PMID:478036", "title": "[Changes in the kallikrein-kinin system of the blood following hypothalamo-hypophyseal stimulation of animals of different ages].", "content": "Changes occurring in the formation and synthesis of kinins become irregular with ageing: the content of kallikreinogenes decreases, the activity of kininase and inhibitors of trypsin falls, and total blood activity of hydrolysis of ethyl--N--benzoil--L--arginine ether rises. Stimulation of the hypothalamus causes an obvious activation of the kalikrein--kinin system in adult animals: the contents of kallikreinogenes and kininogenes fall, the kallikrein activity sharply increases, whereas the kininase activity decreases. Both single and repeated administrations of vasopressin induce the same shifts of kallikrein--kinin system as the stimulation of hypothalamus. On repeated administrations of vasopressin the kininase activity rises.", "contents": "[Changes in the kallikrein-kinin system of the blood following hypothalamo-hypophyseal stimulation of animals of different ages]. Changes occurring in the formation and synthesis of kinins become irregular with ageing: the content of kallikreinogenes decreases, the activity of kininase and inhibitors of trypsin falls, and total blood activity of hydrolysis of ethyl--N--benzoil--L--arginine ether rises. Stimulation of the hypothalamus causes an obvious activation of the kalikrein--kinin system in adult animals: the contents of kallikreinogenes and kininogenes fall, the kallikrein activity sharply increases, whereas the kininase activity decreases. Both single and repeated administrations of vasopressin induce the same shifts of kallikrein--kinin system as the stimulation of hypothalamus. On repeated administrations of vasopressin the kininase activity rises."}
{"id": "PMID:478041", "title": "Isolated epidermolytic acanthoma. A histological study.", "content": "A solitary scrotal lesion of isolated epidermolytic acanthoma was studied by optical microscopy. The lesion displayed typical granular degeneration. The tumor parenchyma comprised several foci extending deep in the cutis to the continuation of the invaginated epithelium. Serial sections showed the parenchyma was free from acrosyringium and epidermis proper. The findings with hematoxylin-eosin stain and Fontana-Masson stain indicated that the tumor had originated from portion B of the hair follicle.", "contents": "Isolated epidermolytic acanthoma. A histological study. A solitary scrotal lesion of isolated epidermolytic acanthoma was studied by optical microscopy. The lesion displayed typical granular degeneration. The tumor parenchyma comprised several foci extending deep in the cutis to the continuation of the invaginated epithelium. Serial sections showed the parenchyma was free from acrosyringium and epidermis proper. The findings with hematoxylin-eosin stain and Fontana-Masson stain indicated that the tumor had originated from portion B of the hair follicle."}
{"id": "PMID:478042", "title": "Pustulosis palmoplantaris and its relation to chronic recurrent multifocal osteomyelitis.", "content": "In previous study we found that clavicular lesions in chronic recurrent multifocal osteomyelitis (CRMO) in young patients was associated with palmoplantar pustulosis (PPP). In this work the relationship between sternoclavicular lesions and PPP was studied in a group of adult patients. The sternoclavicular region of 52 patients with PPP and 25 control persons was examined by tomography. Among the PPP patients there were inflammatory lesions in the sternal synchondroses in 3 patients. There were no such lesions in the controls. In this unselected population of adult patients there was thus no definite association between PPP and sterno-clavicular lesions. In young patients on the other hand, CRMO with clavicular lesions may be another, probably noninfectious, cause of PPP.", "contents": "Pustulosis palmoplantaris and its relation to chronic recurrent multifocal osteomyelitis. In previous study we found that clavicular lesions in chronic recurrent multifocal osteomyelitis (CRMO) in young patients was associated with palmoplantar pustulosis (PPP). In this work the relationship between sternoclavicular lesions and PPP was studied in a group of adult patients. The sternoclavicular region of 52 patients with PPP and 25 control persons was examined by tomography. Among the PPP patients there were inflammatory lesions in the sternal synchondroses in 3 patients. There were no such lesions in the controls. In this unselected population of adult patients there was thus no definite association between PPP and sterno-clavicular lesions. In young patients on the other hand, CRMO with clavicular lesions may be another, probably noninfectious, cause of PPP."}
{"id": "PMID:478045", "title": "[Oral photochemotherapy in atopic eczema].", "content": "11 patients with atopic eczema have been treated using oral photochemotherapy. During the initial phase, complete clearing or more 80% improvements was obtained in 9 of them. However recurrences occured in most of the patients during maintenance therapy. On the whole, failure were observed in 5 patients and good or very good results in 3 patients. 3 patients discontinued therapy after the initial treatment.", "contents": "[Oral photochemotherapy in atopic eczema]. 11 patients with atopic eczema have been treated using oral photochemotherapy. During the initial phase, complete clearing or more 80% improvements was obtained in 9 of them. However recurrences occured in most of the patients during maintenance therapy. On the whole, failure were observed in 5 patients and good or very good results in 3 patients. 3 patients discontinued therapy after the initial treatment."}
{"id": "PMID:478044", "title": "Laboratory investigations in patients with generalized psoriasis under oral retinoid treatment. A multicenter study of computerized data.", "content": "Numerous laboratory parameters were examined 235 patients with generalized psoriasis treated orally with retinoid and in 35 patients treated topically with anthralin as control. Computer evaluation of the obtained data revealed statistical trends to elevation of the total serum bilirubin level and increasing number of blood monocytes after long-term oral treatment. No other statistically significant changes of the laboratory data were found. Particularly, the liver function tests (transaminases, prothrombin and alkaline phosphatase) showed no significant alterations. Only in a few cases did the retinoid compound have an influence on the GPT and GOT levels. The reasons for this individual sensitivity to the drug remain unknown. No significant alterations were found in the control group treated topically with anthralin.", "contents": "Laboratory investigations in patients with generalized psoriasis under oral retinoid treatment. A multicenter study of computerized data. Numerous laboratory parameters were examined 235 patients with generalized psoriasis treated orally with retinoid and in 35 patients treated topically with anthralin as control. Computer evaluation of the obtained data revealed statistical trends to elevation of the total serum bilirubin level and increasing number of blood monocytes after long-term oral treatment. No other statistically significant changes of the laboratory data were found. Particularly, the liver function tests (transaminases, prothrombin and alkaline phosphatase) showed no significant alterations. Only in a few cases did the retinoid compound have an influence on the GPT and GOT levels. The reasons for this individual sensitivity to the drug remain unknown. No significant alterations were found in the control group treated topically with anthralin."}
{"id": "PMID:478047", "title": "Beta-carotene in erythropoietic protoporphyria: 5 years' experience.", "content": "36 patients from 19 families with erythropoietic protoporphyria were treated for about 5 years during the summer months with beta-carotene alone or beta-carotene plus canthaxanthin in daily doses of 50-200 mg. The effect of the treatment was evaluated clinically on the basis of information provided by the patients regarding the period of time they could stay in the sun. 18 patients became completely free of symptoms, 16 patients improved to some extent, and 2 patients noted a slight effect. Apart from the carotenaemia, no side-effects were recorded.", "contents": "Beta-carotene in erythropoietic protoporphyria: 5 years' experience. 36 patients from 19 families with erythropoietic protoporphyria were treated for about 5 years during the summer months with beta-carotene alone or beta-carotene plus canthaxanthin in daily doses of 50-200 mg. The effect of the treatment was evaluated clinically on the basis of information provided by the patients regarding the period of time they could stay in the sun. 18 patients became completely free of symptoms, 16 patients improved to some extent, and 2 patients noted a slight effect. Apart from the carotenaemia, no side-effects were recorded."}
{"id": "PMID:478049", "title": "[Epidermotropic reticulosis of the Woringer-Kolopp type (author's transl)].", "content": "A 46-year-old female, otherwise healthy, developed erythematous, pseudotumoral nodules, mainly on the hands; biopsies led to the diagnosis of Woringer-Kolopp disease. Anti-anergic chemotherapy with levamisole proved hazardous but, possibly, beneficial. Classification into benign localized, fatal generalized and intermediate disease is briefly commented upon. Differential diagnosis and tumor origin are discussed; while the Merkel cell hypothesis has been recently advanced strong analogies and slight discrepancies with mycosis fungoides advocate the T cell lymphoma theory.", "contents": "[Epidermotropic reticulosis of the Woringer-Kolopp type (author's transl)]. A 46-year-old female, otherwise healthy, developed erythematous, pseudotumoral nodules, mainly on the hands; biopsies led to the diagnosis of Woringer-Kolopp disease. Anti-anergic chemotherapy with levamisole proved hazardous but, possibly, beneficial. Classification into benign localized, fatal generalized and intermediate disease is briefly commented upon. Differential diagnosis and tumor origin are discussed; while the Merkel cell hypothesis has been recently advanced strong analogies and slight discrepancies with mycosis fungoides advocate the T cell lymphoma theory."}
{"id": "PMID:478050", "title": "Ultrasound diagnostics as a method of investigation of plastic induration of the penis.", "content": "Using the ultrasound technique of visualization (grey-scale technique) and a method of 'water bath', the authors obtained echograms of plastic indurations of the penis. The echograms can be immediately photographed and interpreted. By their quick, painless and simple performance, without contrast medium and hazardous irradiation, the authors gain an advantage over some other diagnostic methods.", "contents": "Ultrasound diagnostics as a method of investigation of plastic induration of the penis. Using the ultrasound technique of visualization (grey-scale technique) and a method of 'water bath', the authors obtained echograms of plastic indurations of the penis. The echograms can be immediately photographed and interpreted. By their quick, painless and simple performance, without contrast medium and hazardous irradiation, the authors gain an advantage over some other diagnostic methods."}
{"id": "PMID:478051", "title": "Role of macrophages in intraepidermic vesiculation in pemphigus vulgaris. Electron-microscopic observations.", "content": "The authors made an electron-microscopic observation of intraepidermic vesiculation in pemphigus vulgaris at its early stage, and found that numerous macrophages were invading the cleft located immediately superior to the basal cell layer. They suggest that the formation of cleft, as a preceding stage to the eventual development into intraepidermic vesiculation, is intimately related to the squeezing-in of invasive macrophages among keratinocytes.", "contents": "Role of macrophages in intraepidermic vesiculation in pemphigus vulgaris. Electron-microscopic observations. The authors made an electron-microscopic observation of intraepidermic vesiculation in pemphigus vulgaris at its early stage, and found that numerous macrophages were invading the cleft located immediately superior to the basal cell layer. They suggest that the formation of cleft, as a preceding stage to the eventual development into intraepidermic vesiculation, is intimately related to the squeezing-in of invasive macrophages among keratinocytes."}
{"id": "PMID:478052", "title": "[Pemphigus herpetiformis (eosinophilic spongiosis) (author's transl)].", "content": "A 71-year-old male had a clinically atypical pemphigus (pemphigus herpetiformis), associated with histopathologic eosinophilic spongiosis. This case is discussed and was frequently found reported in iatrogenic pemphigus.", "contents": "[Pemphigus herpetiformis (eosinophilic spongiosis) (author's transl)]. A 71-year-old male had a clinically atypical pemphigus (pemphigus herpetiformis), associated with histopathologic eosinophilic spongiosis. This case is discussed and was frequently found reported in iatrogenic pemphigus."}
{"id": "PMID:478053", "title": "Intravenous zinc sulfate therapy in zinc-depleted patients.", "content": "Zinc sulfate was administered intravenously in 3 patients with severe conditional zinc deficiency. The dosage ranged from 10 to 20 mg ionic zinc daily, and the duration of the treatment did not exceed 2 weeks. The rise in serum zinc and urinary zinc per 24 h, as well as in serum alkaline phosphatase, occurred at a faster rate than observed in a patient who was given 135 mg zinc daily by mouth. No subjective or biochemical side effects of the intravenous zinc therapy was observed.", "contents": "Intravenous zinc sulfate therapy in zinc-depleted patients. Zinc sulfate was administered intravenously in 3 patients with severe conditional zinc deficiency. The dosage ranged from 10 to 20 mg ionic zinc daily, and the duration of the treatment did not exceed 2 weeks. The rise in serum zinc and urinary zinc per 24 h, as well as in serum alkaline phosphatase, occurred at a faster rate than observed in a patient who was given 135 mg zinc daily by mouth. No subjective or biochemical side effects of the intravenous zinc therapy was observed."}
{"id": "PMID:478054", "title": "Dose measurement in PUVA therapy.", "content": "The dose emitted by the fluorescent tubes used in PUVA therapy is not constant: it varies in function of time, age of the lamps, and several other factors. Exact knowledge of the dose given to the patient requires continuous measurement and integration of the UV-A output.", "contents": "Dose measurement in PUVA therapy. The dose emitted by the fluorescent tubes used in PUVA therapy is not constant: it varies in function of time, age of the lamps, and several other factors. Exact knowledge of the dose given to the patient requires continuous measurement and integration of the UV-A output."}
{"id": "PMID:478055", "title": "Trichophyton simii infection due to laboratory accident.", "content": "Trichophyton simii produced kerion on the intact forearm skin due to a laboratory accident within 5 days of contact. Early institution of griseofulvin resolved the condition in 15 days with no recurrence during 38 months of follow-up.", "contents": "Trichophyton simii infection due to laboratory accident. Trichophyton simii produced kerion on the intact forearm skin due to a laboratory accident within 5 days of contact. Early institution of griseofulvin resolved the condition in 15 days with no recurrence during 38 months of follow-up."}
{"id": "PMID:478057", "title": "A retrospective study of Patch test results from 163 patients with stasis dermatitis or leg ulcers. I. Discussion of the Patch test results and the sensitization indices and determination of the relevancy of positive reactions.", "content": "Patch test results from 163 patients with leg ulcers and/or chronic venous insufficiency, tested with a modified ICDRG standard test battery and a pharmaceutical test series, are analyzed and compared with the results obtained from a randomly selected control group of patients with eczematous conditions. A sensitization index for the most common contact allergens present in this test series is calculated, and the relevancy of hypersensitivity to specific topical agents is determined.", "contents": "A retrospective study of Patch test results from 163 patients with stasis dermatitis or leg ulcers. I. Discussion of the Patch test results and the sensitization indices and determination of the relevancy of positive reactions. Patch test results from 163 patients with leg ulcers and/or chronic venous insufficiency, tested with a modified ICDRG standard test battery and a pharmaceutical test series, are analyzed and compared with the results obtained from a randomly selected control group of patients with eczematous conditions. A sensitization index for the most common contact allergens present in this test series is calculated, and the relevancy of hypersensitivity to specific topical agents is determined."}
{"id": "PMID:478058", "title": "Polyploidization and hemiploidization induced by PUVA in vivo.", "content": "To obtain additional information on the in vivo injury induced by PUVA treatment, primary cultures were initiated from biopsies of the uninvolved skin of 15 PUVA-treated psoriatic patients, and the cells generated were analysed for morphological and chromosomal modifications. Three biopsies were obtained from each patient, the first one before commencing PUVA therapy (control) and the other two during the course of therapy. It was found that PUVA treatment has diverse effects on the mitotic activity, mitotic mechanisms and chromosomes of the cutaneous cells: (1) an inhibition of cell proliferation during the early stages of therapy followed by a reversion to normal proliferation as the PUVA treatments were continued; the production of (2) cells with more than one nucleus, (3) macrocells, (4) cells with micronuclei, (5) polyploid cells, (6) haploid cells, (7) cells with 13 chromosomes denoting a twofold reduction in the normal number of metaphase chromosomes, (8) end-associations of the chromosomes as in the pachytene phase of meiosis (9) diplotene chromosomes, and (10) chromosomal translocations.", "contents": "Polyploidization and hemiploidization induced by PUVA in vivo. To obtain additional information on the in vivo injury induced by PUVA treatment, primary cultures were initiated from biopsies of the uninvolved skin of 15 PUVA-treated psoriatic patients, and the cells generated were analysed for morphological and chromosomal modifications. Three biopsies were obtained from each patient, the first one before commencing PUVA therapy (control) and the other two during the course of therapy. It was found that PUVA treatment has diverse effects on the mitotic activity, mitotic mechanisms and chromosomes of the cutaneous cells: (1) an inhibition of cell proliferation during the early stages of therapy followed by a reversion to normal proliferation as the PUVA treatments were continued; the production of (2) cells with more than one nucleus, (3) macrocells, (4) cells with micronuclei, (5) polyploid cells, (6) haploid cells, (7) cells with 13 chromosomes denoting a twofold reduction in the normal number of metaphase chromosomes, (8) end-associations of the chromosomes as in the pachytene phase of meiosis (9) diplotene chromosomes, and (10) chromosomal translocations."}
{"id": "PMID:478059", "title": "[Guinea pig nipple as a model for the investigation of melasma (author's transl)].", "content": "The nipples of male tan guinea pigs were used to test the effects on the skin of oral contraceptive pills and drugs. The daily topically applied doses were 1/2,000 of a pill. After 3 weeks, the nipples treated with solutions of ethinyloestradiol-containing pills had grown (200-300%), the epidermis showed acanthosis (150-250%) and strong hyperpigmentation. Mestranol-containing pills as well as pure progestogen substances did not produce these effects.", "contents": "[Guinea pig nipple as a model for the investigation of melasma (author's transl)]. The nipples of male tan guinea pigs were used to test the effects on the skin of oral contraceptive pills and drugs. The daily topically applied doses were 1/2,000 of a pill. After 3 weeks, the nipples treated with solutions of ethinyloestradiol-containing pills had grown (200-300%), the epidermis showed acanthosis (150-250%) and strong hyperpigmentation. Mestranol-containing pills as well as pure progestogen substances did not produce these effects."}
{"id": "PMID:478060", "title": "Becker's melanosis: an ultrastructural study.", "content": "Becker's melanosis is an uncommon condition characterized by hypertrichosis, hyperpigmentation of the epidermis, dermal melanophages and absence of nevus cells. Ultrastructural examination of such a melanotic lesion from a young white man revealed giant melanosomes. In addition, normal-sized melanosomes were found singly in the keratinocytes of the involved skin. Dermal venules were surrounded by multiple layers of basement membrane.", "contents": "Becker's melanosis: an ultrastructural study. Becker's melanosis is an uncommon condition characterized by hypertrichosis, hyperpigmentation of the epidermis, dermal melanophages and absence of nevus cells. Ultrastructural examination of such a melanotic lesion from a young white man revealed giant melanosomes. In addition, normal-sized melanosomes were found singly in the keratinocytes of the involved skin. Dermal venules were surrounded by multiple layers of basement membrane."}
{"id": "PMID:478061", "title": "A retrospective study of patch test results from 163 patients with stasis dermatitis or leg ulcers. II. Retesting of 50 patients.", "content": "Retesting 50 patients with stasis dermatitis after a period of time (3-25 months) revealed significantly less positive patch test results (p less than 0.05). All procedures were, of course, standardized. The main reasons for this were elimination of the sensitization agents in the interim and testing with a less acute skin condition. Allergens that are difficult to avoid, such as the 'para' compounds and balsam of Peru and related substances, show a higher degree of persistence in subsequent patch testing.", "contents": "A retrospective study of patch test results from 163 patients with stasis dermatitis or leg ulcers. II. Retesting of 50 patients. Retesting 50 patients with stasis dermatitis after a period of time (3-25 months) revealed significantly less positive patch test results (p less than 0.05). All procedures were, of course, standardized. The main reasons for this were elimination of the sensitization agents in the interim and testing with a less acute skin condition. Allergens that are difficult to avoid, such as the 'para' compounds and balsam of Peru and related substances, show a higher degree of persistence in subsequent patch testing."}
{"id": "PMID:478062", "title": "Percutaneous absorption of chlorquinaldol (Sterosan).", "content": "The percutaneous absorption of chlorquinaldol from three different preparations for skin treatment (Sterosan-hydrocortison ointment and cream, Sterosan ointment) was evaluated by measuring the cumulative urinary excretion of chlorquinaldol and was compared to that found after an equivalent oral dose. In the blood the concentrations were below the detection limit of the analytical procedure, i.e., smaller than 0.02 microgram/ml. The study was carried out in 3 healthy volunteers using a four-period change-over design. The topical preparations were applied under occlusive dressings. Following epicutaneous application of the three topicals in quantities containing 30 mg chlorquinaldol each, the mean urinary excretion of the drug amounted to 11.1 +/- 6.6, 19.5 +/- 3.5 and 13.0 +/- 2.5% of the applied dose. When the same dose of  chlorquinaldol was administered orally, 67.6 +/- 9.5% of the dose was excreted in the urine. Taking the urinary elimination as the minimal amount of drug absorbed, the extent of percutaneous absorption from the three dermatological preparations varied between 4.2 and 23.5% of the applied dose. There was no difference in the pattern of urinary excretion products among the three topicals and the oral formulation. The bulk of chlorquinaldol (mean 98.0 +/- 0.1%) was excreted as sulfate (mean 1.3 +/- 0.3%) or unchanged drug (mean 0.8 +/- 0.1%).", "contents": "Percutaneous absorption of chlorquinaldol (Sterosan). The percutaneous absorption of chlorquinaldol from three different preparations for skin treatment (Sterosan-hydrocortison ointment and cream, Sterosan ointment) was evaluated by measuring the cumulative urinary excretion of chlorquinaldol and was compared to that found after an equivalent oral dose. In the blood the concentrations were below the detection limit of the analytical procedure, i.e., smaller than 0.02 microgram/ml. The study was carried out in 3 healthy volunteers using a four-period change-over design. The topical preparations were applied under occlusive dressings. Following epicutaneous application of the three topicals in quantities containing 30 mg chlorquinaldol each, the mean urinary excretion of the drug amounted to 11.1 +/- 6.6, 19.5 +/- 3.5 and 13.0 +/- 2.5% of the applied dose. When the same dose of  chlorquinaldol was administered orally, 67.6 +/- 9.5% of the dose was excreted in the urine. Taking the urinary elimination as the minimal amount of drug absorbed, the extent of percutaneous absorption from the three dermatological preparations varied between 4.2 and 23.5% of the applied dose. There was no difference in the pattern of urinary excretion products among the three topicals and the oral formulation. The bulk of chlorquinaldol (mean 98.0 +/- 0.1%) was excreted as sulfate (mean 1.3 +/- 0.3%) or unchanged drug (mean 0.8 +/- 0.1%)."}
{"id": "PMID:478063", "title": "Relationship of pityriasis amiantacea to psoriasis. A follow-up study.", "content": "46 patients with pityriasis aminantacea have been reexamined with regard to the later development of psoriasis and atopic diseases, and their occurrence in first and second degree relatives. HLA antigens of the A, B and C series were also determined. Earlier suggestions of a close association with psoriasis were not confirmed. An association with atopic diseases could not be established. Occurrence of seborrheic dermatitis seems, however, common in patients with pityriasis amiantacea.", "contents": "Relationship of pityriasis amiantacea to psoriasis. A follow-up study. 46 patients with pityriasis aminantacea have been reexamined with regard to the later development of psoriasis and atopic diseases, and their occurrence in first and second degree relatives. HLA antigens of the A, B and C series were also determined. Earlier suggestions of a close association with psoriasis were not confirmed. An association with atopic diseases could not be established. Occurrence of seborrheic dermatitis seems, however, common in patients with pityriasis amiantacea."}
{"id": "PMID:478064", "title": "Chronic pyoderma in splenectomized patient.", "content": "This is a report of a case with a chronic peculiar intractable pyoderma. The process started a year after splenectomy because of trauma. In the absence of an apparent other reason, we assume that a relationship exists between the operation and the skin disease.", "contents": "Chronic pyoderma in splenectomized patient. This is a report of a case with a chronic peculiar intractable pyoderma. The process started a year after splenectomy because of trauma. In the absence of an apparent other reason, we assume that a relationship exists between the operation and the skin disease."}
{"id": "PMID:478065", "title": "An abortive form of pemphigus vulgaris probably induced by penicillin.", "content": "A case of an abortive form of pemphigus probably induced by protracted penicillin treatment is reported. Because of a positive patch test with penicillamine and the finding of such an amino acid in patient's plasma, the authors are induced to believe that the trigger factor could really have been penicillamine formed by the metabolic breakdown of the penicillin molecule.", "contents": "An abortive form of pemphigus vulgaris probably induced by penicillin. A case of an abortive form of pemphigus probably induced by protracted penicillin treatment is reported. Because of a positive patch test with penicillamine and the finding of such an amino acid in patient's plasma, the authors are induced to believe that the trigger factor could really have been penicillamine formed by the metabolic breakdown of the penicillin molecule."}
{"id": "PMID:478066", "title": "[Influence of the stratum corneum on transepidermal water loss and Bioavailability of a new topical steroid, difluprednate: clinical implications (author's transl)].", "content": "Comparative study of experimental and theoretical curves obtained by plotting transepidermal water loss against stratum corneum thickness in man, shows that every layer in the stratum corneum acts as part of the epidermal barrier to water loss. Another function of the stratum corneum is to decrease the cutaneous penetration of topical corticosteroids like difluprednate and to modify their bioavailability ('corticosteroid reservoir'). Our data suggest that variations in stratum corneum thickness between subjects explain variation of transepidermal water loss and sensitivity difluprednate, as there is a close relationship between these two parameters. It is then conceivable than the phototype has clinical implications is there really exists a relationship between phototype, stratum corneum thickness and sensitivity to steroids.", "contents": "[Influence of the stratum corneum on transepidermal water loss and Bioavailability of a new topical steroid, difluprednate: clinical implications (author's transl)]. Comparative study of experimental and theoretical curves obtained by plotting transepidermal water loss against stratum corneum thickness in man, shows that every layer in the stratum corneum acts as part of the epidermal barrier to water loss. Another function of the stratum corneum is to decrease the cutaneous penetration of topical corticosteroids like difluprednate and to modify their bioavailability ('corticosteroid reservoir'). Our data suggest that variations in stratum corneum thickness between subjects explain variation of transepidermal water loss and sensitivity difluprednate, as there is a close relationship between these two parameters. It is then conceivable than the phototype has clinical implications is there really exists a relationship between phototype, stratum corneum thickness and sensitivity to steroids."}
{"id": "PMID:478067", "title": "Leucocyte migration inhibition in Beh\u00e7et's disease.", "content": "The leucocyte migration inhibition (LIF) was investigated in 16 patients with Beh\u00e7et's disease, 5 while in the active stage of the process, 3 both during relapse and remission, and 8 during remission of symptoms. In all patients skin tests with various antigens and with a needle prick and physiological saline were simultaneously performed. The results indicate a strong correlation between the activity of the disease, skin reactivity and the reactivity of LIF.", "contents": "Leucocyte migration inhibition in Beh\u00e7et's disease. The leucocyte migration inhibition (LIF) was investigated in 16 patients with Beh\u00e7et's disease, 5 while in the active stage of the process, 3 both during relapse and remission, and 8 during remission of symptoms. In all patients skin tests with various antigens and with a needle prick and physiological saline were simultaneously performed. The results indicate a strong correlation between the activity of the disease, skin reactivity and the reactivity of LIF."}
{"id": "PMID:478068", "title": "Epidermal hyperplasia induced in guinea pig flank skin by intradermal injection of Sudan red.", "content": "Studies of dermal-epidermal interactions were conducted with guinea pig flank skin and intradermal injections of the irritant, Sudan IV dye in olive oil. These injections led to epidermal hyperplasia in areas overlying the irritant and the effect was most significant when the irritant was placed in the upper dermis. Basal cell mitotic activity and thymidine uptake reached a peak by 24 h and thereafter dropped rapidly. Maximal epidermal thickness (4.3 times the control) resulting from an increase in cell number occurred within 2-4 days. Despite the very short period of increased cell growth, epidermal thickness returned to control values only after a 24-day period. A similar growth response could not be induced by saline injections. A single topical application of the irritant showed a qualitatively and quantitatively different epidermal response. These experiments indicate that an intradermal irritant can lead to epidermal hyperplasia and a long-lasting epidermal thickening.", "contents": "Epidermal hyperplasia induced in guinea pig flank skin by intradermal injection of Sudan red. Studies of dermal-epidermal interactions were conducted with guinea pig flank skin and intradermal injections of the irritant, Sudan IV dye in olive oil. These injections led to epidermal hyperplasia in areas overlying the irritant and the effect was most significant when the irritant was placed in the upper dermis. Basal cell mitotic activity and thymidine uptake reached a peak by 24 h and thereafter dropped rapidly. Maximal epidermal thickness (4.3 times the control) resulting from an increase in cell number occurred within 2-4 days. Despite the very short period of increased cell growth, epidermal thickness returned to control values only after a 24-day period. A similar growth response could not be induced by saline injections. A single topical application of the irritant showed a qualitatively and quantitatively different epidermal response. These experiments indicate that an intradermal irritant can lead to epidermal hyperplasia and a long-lasting epidermal thickening."}
{"id": "PMID:478069", "title": "[Multiple familial follicular hamartoma (author's transl)].", "content": "Multiple cystic and proliferative follicular lesions localized on the face and the genitalia of several members of an Italian family are described. Transmission seems to be autosomal dominant, with weak penetration and variable expressivity.", "contents": "[Multiple familial follicular hamartoma (author's transl)]. Multiple cystic and proliferative follicular lesions localized on the face and the genitalia of several members of an Italian family are described. Transmission seems to be autosomal dominant, with weak penetration and variable expressivity."}
{"id": "PMID:478070", "title": "Porokeratosis of Mibelli. A report of two unusual cases.", "content": "2 cases are reported with unusual manifestations of porokeratosis of Mibelli. In 1 case multiple atrophic facial lesions resembled plaques of chronic discoid lupus erythematosus. The second case exhibited extensive cutaneous involvement in the form of hyperkeratotic lesions in linear configuration.", "contents": "Porokeratosis of Mibelli. A report of two unusual cases. 2 cases are reported with unusual manifestations of porokeratosis of Mibelli. In 1 case multiple atrophic facial lesions resembled plaques of chronic discoid lupus erythematosus. The second case exhibited extensive cutaneous involvement in the form of hyperkeratotic lesions in linear configuration."}
{"id": "PMID:478071", "title": "Two primary malignant melanomas in a patient with plasmacytoma.", "content": "The occurrence of two primary malignant melanomas (superficial spreading melanoma, superficial spreading melanoma with nodular growth) in a patient with diffuse plasmacytoma treated with chemotherapy and radiation is described. Such an association does not appear to have been reported previously.", "contents": "Two primary malignant melanomas in a patient with plasmacytoma. The occurrence of two primary malignant melanomas (superficial spreading melanoma, superficial spreading melanoma with nodular growth) in a patient with diffuse plasmacytoma treated with chemotherapy and radiation is described. Such an association does not appear to have been reported previously."}
{"id": "PMID:478072", "title": "Nevus unius lateralis exhibiting intraepidermal basaloid cell formation simulating superficial basal cell epithelioma.", "content": "A unilateral verrucous lesion with clinical characteristics of nevus unius lateralis (NUL) in an 18-year-old boy, showed histopathological features of intraepidermal basaloid cell formation simulating superficial basal cell epitheliomas. Biological significance of this phenomenon is discussed. The lesion is considered a most peculiar variant of NUL.", "contents": "Nevus unius lateralis exhibiting intraepidermal basaloid cell formation simulating superficial basal cell epithelioma. A unilateral verrucous lesion with clinical characteristics of nevus unius lateralis (NUL) in an 18-year-old boy, showed histopathological features of intraepidermal basaloid cell formation simulating superficial basal cell epitheliomas. Biological significance of this phenomenon is discussed. The lesion is considered a most peculiar variant of NUL."}
{"id": "PMID:478073", "title": "Keratosis follicularis and familial benign chronic pemphigus in the same patient.", "content": "A case is described with features both of Hailey-Hailey and Darier's disease in a male patient who presented skin lesions since he was 8 years old. The present case is discussed in relation to data obtained from the literature, and a differential diagnosis from Grover's disease is made.", "contents": "Keratosis follicularis and familial benign chronic pemphigus in the same patient. A case is described with features both of Hailey-Hailey and Darier's disease in a male patient who presented skin lesions since he was 8 years old. The present case is discussed in relation to data obtained from the literature, and a differential diagnosis from Grover's disease is made."}
{"id": "PMID:478074", "title": "Coincidence of vitiligo, alopecia areata, onychodystrophy, localized scleroderma and lichen planus.", "content": "The unique coincidence of five dermatological disorders, which occurred in a 39-year-old patient, is discussed. The clinical and laboratory examination did not reveal a common underlying cause. It is hoped this report will stimulate the recognition of other cases and thus aid in determining whether the coincidence of these disorders is a true association of diseases with a common underlying factor or a rare abnormality.", "contents": "Coincidence of vitiligo, alopecia areata, onychodystrophy, localized scleroderma and lichen planus. The unique coincidence of five dermatological disorders, which occurred in a 39-year-old patient, is discussed. The clinical and laboratory examination did not reveal a common underlying cause. It is hoped this report will stimulate the recognition of other cases and thus aid in determining whether the coincidence of these disorders is a true association of diseases with a common underlying factor or a rare abnormality."}
{"id": "PMID:478080", "title": "Effects of long term sulfonylurea therapy on plasma insulin and fasting lipid levels.", "content": "Insulin secretion was studied before and after the control of hyperglycemia in fourteen maturity onset male non-obese diabetics. Optimum control of hyperglycemia was achieved by the addition of the sulfonylurea chlorpropamide to dietary treatment. One patient was a primary treatment failure, but nine out of thirteen had excellent control of hyperglycemia. A standardized oral glucose tolerance test (GTT) was performed before and after eight months of individualized therapy with the sulfonylurea. The GTT was repeated with each patient taking his usual dose of chlorpropamide 90 min prior to the administrationo f the glucose load. In the baseline test glucose levels rose from 135.6 +/- 9.9 mg/dl to a peak level of 268.8 +/- 17.7 mg/dl at 120 min. After control of hyperglycemia glucose levels were significantly lower at 0, 30 and 60 min, and rose from 106.8 +/- 8.5 mg/dl to a maximum of 224.5 +/- 17.3 mg/dl at 120 min. Plasma insulin response was unchanged. Fasting serum cholesterol, triglyceride and total lipid levels changed only minimally during therapy. It is concluded that lowered serum glucose levels after long term treatment with chlorpropamide occured while plasma insulin response to glucose was no greater than before treatment. These findings may be explained by an extrapancreatic effect of the drug or by an indirect result of chlorpropamide induced insulin release which occured earlier in the course of therapy.", "contents": "Effects of long term sulfonylurea therapy on plasma insulin and fasting lipid levels. Insulin secretion was studied before and after the control of hyperglycemia in fourteen maturity onset male non-obese diabetics. Optimum control of hyperglycemia was achieved by the addition of the sulfonylurea chlorpropamide to dietary treatment. One patient was a primary treatment failure, but nine out of thirteen had excellent control of hyperglycemia. A standardized oral glucose tolerance test (GTT) was performed before and after eight months of individualized therapy with the sulfonylurea. The GTT was repeated with each patient taking his usual dose of chlorpropamide 90 min prior to the administrationo f the glucose load. In the baseline test glucose levels rose from 135.6 +/- 9.9 mg/dl to a peak level of 268.8 +/- 17.7 mg/dl at 120 min. After control of hyperglycemia glucose levels were significantly lower at 0, 30 and 60 min, and rose from 106.8 +/- 8.5 mg/dl to a maximum of 224.5 +/- 17.3 mg/dl at 120 min. Plasma insulin response was unchanged. Fasting serum cholesterol, triglyceride and total lipid levels changed only minimally during therapy. It is concluded that lowered serum glucose levels after long term treatment with chlorpropamide occured while plasma insulin response to glucose was no greater than before treatment. These findings may be explained by an extrapancreatic effect of the drug or by an indirect result of chlorpropamide induced insulin release which occured earlier in the course of therapy."}
{"id": "PMID:478081", "title": "Metabolic studies in a case of Werner's syndrome.", "content": "A case of Werner's syndrome was investigated with regard to the immune system and peripheral insulin sensitivity. Routine immunological screening revealed no abnormality in either humoral or cell-mediated immune response. Intravenous glucose tolerance test showed a reduced glucose tolerance coupled with very high insulin levels. There was no evidence for increased levels of antagonistic hormones e.g., corticosteroids, growth hormone or thyroid hormones. Serum insulin binding capacity was normal. Adipose tissue metabolism revealed a reduced sensitivity to the effects of insulin both on glucose metabolism and on the antilipolytic effect. This was purely due to a shift in the dose-response curve to the right since maximal effects were the same as those found in control subjects. This reduced insulin sensitivity seems to be due to a reduction in the number of cellular insulin receptors.", "contents": "Metabolic studies in a case of Werner's syndrome. A case of Werner's syndrome was investigated with regard to the immune system and peripheral insulin sensitivity. Routine immunological screening revealed no abnormality in either humoral or cell-mediated immune response. Intravenous glucose tolerance test showed a reduced glucose tolerance coupled with very high insulin levels. There was no evidence for increased levels of antagonistic hormones e.g., corticosteroids, growth hormone or thyroid hormones. Serum insulin binding capacity was normal. Adipose tissue metabolism revealed a reduced sensitivity to the effects of insulin both on glucose metabolism and on the antilipolytic effect. This was purely due to a shift in the dose-response curve to the right since maximal effects were the same as those found in control subjects. This reduced insulin sensitivity seems to be due to a reduction in the number of cellular insulin receptors."}
{"id": "PMID:478082", "title": "Lipogenesis in human adipose tissue in vitro: effect of fat cell size on some enzymatic activities.", "content": "The incorporation of [1-C14] palmitate into palmitoyl CoA and triglycerides by homogenates of human adipose tissue have been studied. Adipose tissue samples were taken from three sites varying in adipocytes size (omentum, subcutaneous abdominal wall, buttock). The donors were normal weight women of constant weight. A significant positive correlation was found between initial velocity of palmitoyl CoA synthetase (EC 6.2.1.3) and total [1-C14] palmitate incorporation into triglycerides on one hand and adipocyte cell size on the other hand : these relations with cell size were apparent both within and between individuals. The mechanism of this \"size effect\" which is unrelated to the higher protein content of larger cells, is still unexplained. Lipogenesis, like most of the metabolic activities of adipose tissue increases with enlarging cell size. Acceleration of a lipogenesis-lipolysis cycle could constitute an energy wasting system able to limit the volume of the adipocytes.", "contents": "Lipogenesis in human adipose tissue in vitro: effect of fat cell size on some enzymatic activities. The incorporation of [1-C14] palmitate into palmitoyl CoA and triglycerides by homogenates of human adipose tissue have been studied. Adipose tissue samples were taken from three sites varying in adipocytes size (omentum, subcutaneous abdominal wall, buttock). The donors were normal weight women of constant weight. A significant positive correlation was found between initial velocity of palmitoyl CoA synthetase (EC 6.2.1.3) and total [1-C14] palmitate incorporation into triglycerides on one hand and adipocyte cell size on the other hand : these relations with cell size were apparent both within and between individuals. The mechanism of this \"size effect\" which is unrelated to the higher protein content of larger cells, is still unexplained. Lipogenesis, like most of the metabolic activities of adipose tissue increases with enlarging cell size. Acceleration of a lipogenesis-lipolysis cycle could constitute an energy wasting system able to limit the volume of the adipocytes."}
{"id": "PMID:478083", "title": "Oral glucose in cirrhotics. Effects on plasma aminoacid patterns and the role of insulin and glucagon.", "content": "Changes in the plasma aminoacid (AA) profile present in hepatic encephalopathy were related to a catabolic state characterized by a reduced insulin/glucagon molar ratio (IRI/IRG). Oral glucose is able to suppress the hyperglucagonemia and further to increase the elevated insulin levels of cirrhotics leading to a rise of IRI/IRG. We evaluated the plasma AAs in ten controls and twelve cirrhotics following the ingestion of oral glucose. At 180 min we demonstrated a similar fall (about 35%) of plasma AAs both in cirrhotics and in controls, with the exception of free tryptophan, which fell more markedly in cirrhotics (about 60%), possibly secondary to the fall in plasma free fatty acids. After the oral glucose load, the levels of aromatic AAs and free tryptophan, as well as the molar ratio free tryptophan/branched-chain + aromatic AAs returned to normal in cirrhotics. High levels of both aromatic AAs and free tryptophan have been implicated in the pathogenesis of hepatic coma. Our data support the hypothesis that the administration of oral glucose might be relevant in the management of cirrhotic patients with hepatic encephalopathy, possibly improving their mental state.", "contents": "Oral glucose in cirrhotics. Effects on plasma aminoacid patterns and the role of insulin and glucagon. Changes in the plasma aminoacid (AA) profile present in hepatic encephalopathy were related to a catabolic state characterized by a reduced insulin/glucagon molar ratio (IRI/IRG). Oral glucose is able to suppress the hyperglucagonemia and further to increase the elevated insulin levels of cirrhotics leading to a rise of IRI/IRG. We evaluated the plasma AAs in ten controls and twelve cirrhotics following the ingestion of oral glucose. At 180 min we demonstrated a similar fall (about 35%) of plasma AAs both in cirrhotics and in controls, with the exception of free tryptophan, which fell more markedly in cirrhotics (about 60%), possibly secondary to the fall in plasma free fatty acids. After the oral glucose load, the levels of aromatic AAs and free tryptophan, as well as the molar ratio free tryptophan/branched-chain + aromatic AAs returned to normal in cirrhotics. High levels of both aromatic AAs and free tryptophan have been implicated in the pathogenesis of hepatic coma. Our data support the hypothesis that the administration of oral glucose might be relevant in the management of cirrhotic patients with hepatic encephalopathy, possibly improving their mental state."}
{"id": "PMID:478084", "title": "A-Cell and gut glucagon in normal and depancreatized dogs. Inhibition by somatostatin and insulin.", "content": "The effect of somatostatin (SRIF) and of insulin on the plasma levels of immunoreactive glucagon (IRG) and glucose was examined in normal (N) and depancreatized (PX) dogs. The infusion of SRIF (3 microgram/min for 15 min) caused a rapid decrease of the total IRG measured by means of an antiglucanon serum (AGS 10) which cross reacts with extracts of intestinal mucosa. This decrease was due primarily to a fall in the IRG fraction measured by an antiserum (AGS 18) specific for the carboxyl terminus of pancreatic or A-cell IRG. When the dose of SRIF was increased to 10 microgram/min for 90 min, the difference between total and A-cell IRG in the systemic blood also decreased, indicating that other IRG fractions, such as gut IRG, had also been suppressed. The introduction of 50 ml of a 5% glucose solution into a loop of ileum was followed by an increase of gut IRG measured in the regional mesenteric blood. This response was suppressed by the infusion of SRIF (3 microgram/min). Insulin suppressed the basal level of total IRG, but did not alter the gut IRG response to glucose. The SRIF- and insulin-induced reduction in plasma IRG was not associated with a reduction in plasma glucose, suggesting that the high levels of total and A-cell IRG observed in depancreatized dogs were not essential for the maintenance of hyperglycemia.", "contents": "A-Cell and gut glucagon in normal and depancreatized dogs. Inhibition by somatostatin and insulin. The effect of somatostatin (SRIF) and of insulin on the plasma levels of immunoreactive glucagon (IRG) and glucose was examined in normal (N) and depancreatized (PX) dogs. The infusion of SRIF (3 microgram/min for 15 min) caused a rapid decrease of the total IRG measured by means of an antiglucanon serum (AGS 10) which cross reacts with extracts of intestinal mucosa. This decrease was due primarily to a fall in the IRG fraction measured by an antiserum (AGS 18) specific for the carboxyl terminus of pancreatic or A-cell IRG. When the dose of SRIF was increased to 10 microgram/min for 90 min, the difference between total and A-cell IRG in the systemic blood also decreased, indicating that other IRG fractions, such as gut IRG, had also been suppressed. The introduction of 50 ml of a 5% glucose solution into a loop of ileum was followed by an increase of gut IRG measured in the regional mesenteric blood. This response was suppressed by the infusion of SRIF (3 microgram/min). Insulin suppressed the basal level of total IRG, but did not alter the gut IRG response to glucose. The SRIF- and insulin-induced reduction in plasma IRG was not associated with a reduction in plasma glucose, suggesting that the high levels of total and A-cell IRG observed in depancreatized dogs were not essential for the maintenance of hyperglycemia."}
{"id": "PMID:478085", "title": "Diabetic mortality in Edinburgh.", "content": "A prospective study of mortality in 3,113 diabetics was carried out in Edinburgh over a period of eight years; 1,272 patients (41 %) died. Death rates for females equalled those for males and, in relation to the general population, there was a considerable excess mortality which was greater for females. Statistical analysis indicated that the important mortality risk-factors are age, duration of diabetes of greater than ten years and treatment. The risk of oral therapy or insulin were approximately equally greater than that of diet therapy and probably reflected severity of disease. Using international coding for diagnosis, 27 % of deaths were classified as directly due to diabetes and 49 % to vascular disease. Reclassifying the terminal cause of death left only 26 patients (2 %) recorded with diabetes as the direct cause of death. Three hundred and thirty five males (66 %) and 561 females (73 %) died of vascular disease. There was a predominance of myocardial infarction in males and cerebrovascular disease in females. These percentages were a little lower when post-mortem information was available. These results provide additional evidence that diabetes reduces life expectancy by inducing premature vascular disease and that the effect is greater in women than in men.", "contents": "Diabetic mortality in Edinburgh. A prospective study of mortality in 3,113 diabetics was carried out in Edinburgh over a period of eight years; 1,272 patients (41 %) died. Death rates for females equalled those for males and, in relation to the general population, there was a considerable excess mortality which was greater for females. Statistical analysis indicated that the important mortality risk-factors are age, duration of diabetes of greater than ten years and treatment. The risk of oral therapy or insulin were approximately equally greater than that of diet therapy and probably reflected severity of disease. Using international coding for diagnosis, 27 % of deaths were classified as directly due to diabetes and 49 % to vascular disease. Reclassifying the terminal cause of death left only 26 patients (2 %) recorded with diabetes as the direct cause of death. Three hundred and thirty five males (66 %) and 561 females (73 %) died of vascular disease. There was a predominance of myocardial infarction in males and cerebrovascular disease in females. These percentages were a little lower when post-mortem information was available. These results provide additional evidence that diabetes reduces life expectancy by inducing premature vascular disease and that the effect is greater in women than in men."}
{"id": "PMID:478086", "title": "High molecular weight substances with insulin immunoreactivity.", "content": "Substances which react with anti-insulin antibodies, but have a higher molecular weight than insulin are described. They have been studied by various authors in pancreatic adenomas, pancreas extracts, serum and cell-free systems. Proinsulin was the first of these products to be discovered, followed by its precursor, pre-proinsulin, and subsequently by a whole series of substances of ill-defined composition and more or less similar properties, grouped under the heading of \"high molecular weight immunoreactive insulin\" (HWIRI).", "contents": "High molecular weight substances with insulin immunoreactivity. Substances which react with anti-insulin antibodies, but have a higher molecular weight than insulin are described. They have been studied by various authors in pancreatic adenomas, pancreas extracts, serum and cell-free systems. Proinsulin was the first of these products to be discovered, followed by its precursor, pre-proinsulin, and subsequently by a whole series of substances of ill-defined composition and more or less similar properties, grouped under the heading of \"high molecular weight immunoreactive insulin\" (HWIRI)."}
{"id": "PMID:478088", "title": "Fluorescein angiography in borderline diabetics.", "content": "Fluorescein angiography was performed in twenty three men with impaired oral glucose tolerance (borderline diabetics) and sixteen matched, normoglycaemic controls. Retinal abnormalities (microaneurysms and leakage of dye) were observed in 18.7% of the controls, 41.6% of sustained borderline diabetics and 54.4% of initially borderline diabetics who subsequently \"worsened to diabetes\". Qualitatively the abnormalities were similar in each group. Although the difference in frequency between the sustained borderline diabetics and the controls does not achieve conventional statistical significance, other studies quoted support the conclusion that moderate impairment of glucose tolerance is associated with a raised frequency of minor retinal abnormalities. The significance of these remains to be determined.", "contents": "Fluorescein angiography in borderline diabetics. Fluorescein angiography was performed in twenty three men with impaired oral glucose tolerance (borderline diabetics) and sixteen matched, normoglycaemic controls. Retinal abnormalities (microaneurysms and leakage of dye) were observed in 18.7% of the controls, 41.6% of sustained borderline diabetics and 54.4% of initially borderline diabetics who subsequently \"worsened to diabetes\". Qualitatively the abnormalities were similar in each group. Although the difference in frequency between the sustained borderline diabetics and the controls does not achieve conventional statistical significance, other studies quoted support the conclusion that moderate impairment of glucose tolerance is associated with a raised frequency of minor retinal abnormalities. The significance of these remains to be determined."}
{"id": "PMID:478089", "title": "Retinal fluorescein angiography and hemoglobin A1C in borderline diabetes.", "content": "In a controlled study fluorescein angiography of the retina and measurements of hemoglobin A1C were carried out in subjects with slightly abnormal two-hour glucose tolerance and controls with normal glucose tolerance. Fluorescent dots were present in 50 per cent of the series with a reduced glucose tolerance. Leakage from retinal vessels was noted in 20 per cent. In the control group fluorescent dots were present in 25 per cent, of whom none showed leakage. In neither of the series did any subject display ophthalmoscopically visible signs of diabetic retinopathy. In the subjects with abnormal glucose tolerance the mean concentration of hemoglobin A1C was 5.88% +/- 1.15 (SD) and in the control group 5.11% +/- 0.85 (SD) (P less than 0.05) . No correlation was demonstrable between the number of fluorescent dots, hemoglobin A1C and age in the group with abnormal glucose tolerance, but a relationship was noticed between a hereditary disposition to diabetes mellitus and presence of fluorescent dots. Smoking appeared not to have any influence. The result of the present study indicates that incipient retinal microvascular changes and evidence of an early breakdown of the blood-retinal barrier may be present in subjects before diabetes becomes manifest.", "contents": "Retinal fluorescein angiography and hemoglobin A1C in borderline diabetes. In a controlled study fluorescein angiography of the retina and measurements of hemoglobin A1C were carried out in subjects with slightly abnormal two-hour glucose tolerance and controls with normal glucose tolerance. Fluorescent dots were present in 50 per cent of the series with a reduced glucose tolerance. Leakage from retinal vessels was noted in 20 per cent. In the control group fluorescent dots were present in 25 per cent, of whom none showed leakage. In neither of the series did any subject display ophthalmoscopically visible signs of diabetic retinopathy. In the subjects with abnormal glucose tolerance the mean concentration of hemoglobin A1C was 5.88% +/- 1.15 (SD) and in the control group 5.11% +/- 0.85 (SD) (P less than 0.05) . No correlation was demonstrable between the number of fluorescent dots, hemoglobin A1C and age in the group with abnormal glucose tolerance, but a relationship was noticed between a hereditary disposition to diabetes mellitus and presence of fluorescent dots. Smoking appeared not to have any influence. The result of the present study indicates that incipient retinal microvascular changes and evidence of an early breakdown of the blood-retinal barrier may be present in subjects before diabetes becomes manifest."}
{"id": "PMID:478159", "title": "Netilmicin: clinical evaluation of efficacy and toxicity of a new aminoglycoside.", "content": "The efficacy and toxicity of netilmicin, a new semisynthetic aminoglycoside, was clinically evaluated in fifty-two patients with moderate to severe infections with Gram-negative rods or Staph. aureus. Average duration of treatment was 14 days and mean total dose 2,960 mg. One-hour mean value of netilmicin serum concentration was 6.4 microgram/ml and mean trough value 1.2 microgram/ml. Forty-four patients were cured or improved. In twenty-one of them the effect could be attributed to netilmicin alone; the other twenty-three had a combined therapy. No improvement took place in five, but four of them could not be regarded as netilmicin failure. One patient with Pseudomonas aeruginosa infection was possible failure. The sensitivity of the causative bacteria to netilmicin was studied and compared with amikacin, gentamicin, sisomicin and tobramycin. Vestibular function and hearing acuity was thoroughly examined by electronystagmography and audiography. Drug-related VIIIth nerve damage could not be confirmed in any of our patients. Five patients showed a rise of serum creatinine of 30 mumol/l. This shows that netilmicin, similar to other aminoglycosides, is a potential nephrotoxic drug. Netilmicin appears to be an efficacious aminoglycoside and the oto- and nephrotoxicity is low, if careful attention is paid to the renal function and the serum concentrations of the drug.", "contents": "Netilmicin: clinical evaluation of efficacy and toxicity of a new aminoglycoside. The efficacy and toxicity of netilmicin, a new semisynthetic aminoglycoside, was clinically evaluated in fifty-two patients with moderate to severe infections with Gram-negative rods or Staph. aureus. Average duration of treatment was 14 days and mean total dose 2,960 mg. One-hour mean value of netilmicin serum concentration was 6.4 microgram/ml and mean trough value 1.2 microgram/ml. Forty-four patients were cured or improved. In twenty-one of them the effect could be attributed to netilmicin alone; the other twenty-three had a combined therapy. No improvement took place in five, but four of them could not be regarded as netilmicin failure. One patient with Pseudomonas aeruginosa infection was possible failure. The sensitivity of the causative bacteria to netilmicin was studied and compared with amikacin, gentamicin, sisomicin and tobramycin. Vestibular function and hearing acuity was thoroughly examined by electronystagmography and audiography. Drug-related VIIIth nerve damage could not be confirmed in any of our patients. Five patients showed a rise of serum creatinine of 30 mumol/l. This shows that netilmicin, similar to other aminoglycosides, is a potential nephrotoxic drug. Netilmicin appears to be an efficacious aminoglycoside and the oto- and nephrotoxicity is low, if careful attention is paid to the renal function and the serum concentrations of the drug."}
{"id": "PMID:478160", "title": "Amoxycillin injection in the treatment of pyogenic meningitis in children.", "content": "An open trial of the use of amoxycillin injection in the treatment of pyogenic meningitis in children showed similar results to previous trials here of penicillin-chloramphenicol combinations. It is felt that, in developing countries at least, treatment of pyogenic meningitis in children should be with the latter combination, but where rapid and accurate bacteriology is available amoxycillin may be valuable non-toxic alternative to chloramphenicol with similar activity to ampicillin.", "contents": "Amoxycillin injection in the treatment of pyogenic meningitis in children. An open trial of the use of amoxycillin injection in the treatment of pyogenic meningitis in children showed similar results to previous trials here of penicillin-chloramphenicol combinations. It is felt that, in developing countries at least, treatment of pyogenic meningitis in children should be with the latter combination, but where rapid and accurate bacteriology is available amoxycillin may be valuable non-toxic alternative to chloramphenicol with similar activity to ampicillin."}
{"id": "PMID:478161", "title": "A month-by-month long-term study on carbamazepine:clinical, EEG and pharmacological evaluation.", "content": "Carbamazephine plasma levels were monitored for a twelve-month period in twenty epileptic patients. Seizure frequency decreased remarkably on inclusion of the drug in the therapy and attainment of plasma levels of 7-9 microgram/ml. A faster breakdown rate in children was confirmed, while carbamazepine-10, 11-epoxide was present for carbamazepine levels above 4-5 microgram/ml. EEG tracings did not show substantial modifications; this variable was thus considered to be of little value in assessing the clinical picture. Side-effects were minimal. Carbamazepine is increasingly becoming the drug of first choice in the management of both generalized and partial epilepsy, while better and more rapid results are obtained in monotherapy.", "contents": "A month-by-month long-term study on carbamazepine:clinical, EEG and pharmacological evaluation. Carbamazephine plasma levels were monitored for a twelve-month period in twenty epileptic patients. Seizure frequency decreased remarkably on inclusion of the drug in the therapy and attainment of plasma levels of 7-9 microgram/ml. A faster breakdown rate in children was confirmed, while carbamazepine-10, 11-epoxide was present for carbamazepine levels above 4-5 microgram/ml. EEG tracings did not show substantial modifications; this variable was thus considered to be of little value in assessing the clinical picture. Side-effects were minimal. Carbamazepine is increasingly becoming the drug of first choice in the management of both generalized and partial epilepsy, while better and more rapid results are obtained in monotherapy."}
{"id": "PMID:478162", "title": "Domperidone in the treatment of dyspepsia: a double-blind placebo-controlled study.", "content": "Domperidone, at a dosage of 20 mg t.d.s before meals, in a double-blind, crossover, placebo-controlled trial reduced the level of the symptoms of dyspepsia by 76% compared to a 16% reduction with placebo. This difference was statistically significant (p less than 0.001). Thirteen of the fourteen patients in the study preferred domperidone to placebo. Four patients in the active treatment period and one in the placebo complained of mild side-effects.", "contents": "Domperidone in the treatment of dyspepsia: a double-blind placebo-controlled study. Domperidone, at a dosage of 20 mg t.d.s before meals, in a double-blind, crossover, placebo-controlled trial reduced the level of the symptoms of dyspepsia by 76% compared to a 16% reduction with placebo. This difference was statistically significant (p less than 0.001). Thirteen of the fourteen patients in the study preferred domperidone to placebo. Four patients in the active treatment period and one in the placebo complained of mild side-effects."}
{"id": "PMID:478163", "title": "A schedule for combined intravenous and oral dosing with mexiletine.", "content": "A combined intravenous and oral dosage regime for mexiletine has been evaluated in volunteer subjects. Therapeutically effective plasma concentrations of the drug were achieved rapidly and maintained over a four-hour period. Side-effects were minimal and the scheme would be suitable for the anti-arrhythmic protection of patients being transported to hospital and for the treatment of patients receiving hospital or, even, domicilliary care.", "contents": "A schedule for combined intravenous and oral dosing with mexiletine. A combined intravenous and oral dosage regime for mexiletine has been evaluated in volunteer subjects. Therapeutically effective plasma concentrations of the drug were achieved rapidly and maintained over a four-hour period. Side-effects were minimal and the scheme would be suitable for the anti-arrhythmic protection of patients being transported to hospital and for the treatment of patients receiving hospital or, even, domicilliary care."}
{"id": "PMID:478174", "title": "Ontogenetic development of pentobarbital-induced EEG pattern and sleeping time in rats.", "content": "The latency of onset and the duration of sleep induced by pentobarbital was studied in rats from 9 to 35 days of age, as well as in adult rats. Sleep latency was prolonged and sleep duration shortened with increasing age and maturation. Electrocorticographic changes after pentobarbital administration were studied in rats of 5-45 days of age and in adult rats. In rats 9 days of age or less the electrocorticogram manifested only depression of activity, whereas from the 12th day onward all characteristic barbiturate-induced phenomena were registered: spindles, slow waves, and isolated spikes with suppression of background activity. Spindles were the most conspicuous of the pentobarbital-induced phenomena and were the 1st to appear as a function of age, occurring only in the frontal areas of 12 days of age but in both frontal and occipital areas at 15 days of age. Frequency of the elements comprising spindles increased with from 2.5-3.5 c/sec in 12-day-old rats to 5-10 c/sec in adult animals. Barbiturate spindles could be used as a model for rhythmic thalamocortical phenomena even at early stages of development.", "contents": "Ontogenetic development of pentobarbital-induced EEG pattern and sleeping time in rats. The latency of onset and the duration of sleep induced by pentobarbital was studied in rats from 9 to 35 days of age, as well as in adult rats. Sleep latency was prolonged and sleep duration shortened with increasing age and maturation. Electrocorticographic changes after pentobarbital administration were studied in rats of 5-45 days of age and in adult rats. In rats 9 days of age or less the electrocorticogram manifested only depression of activity, whereas from the 12th day onward all characteristic barbiturate-induced phenomena were registered: spindles, slow waves, and isolated spikes with suppression of background activity. Spindles were the most conspicuous of the pentobarbital-induced phenomena and were the 1st to appear as a function of age, occurring only in the frontal areas of 12 days of age but in both frontal and occipital areas at 15 days of age. Frequency of the elements comprising spindles increased with from 2.5-3.5 c/sec in 12-day-old rats to 5-10 c/sec in adult animals. Barbiturate spindles could be used as a model for rhythmic thalamocortical phenomena even at early stages of development."}
{"id": "PMID:478175", "title": "Differential extinction of 2-way active avoidance in young and adult rats.", "content": "Three experiments examined age-related differences in extinction of active avoidance in rats through manipulation of the stimulus context. In Experiment I, 36 weanlings and 36 adults acquired comparable avoidance levels to a tonal conditioned stimulus (CS), and novel stimuli consisting of either the presence of a naive rat or a light were presented during 2 tests in extinction. Results indicated more pronounced disruption of extinction in the pups, compared to adults, with the animate novel stimulus having the greater effect. Eighteen pups of 22 days of age and 18 adults in Experiment II were trained to avoid shock with a CS consisting of simultaneous tone and light onset. During extinction responses did not terminate 1 of the CS elements, but rather produced continuation of either the tone or light for an additional 5 sec. Continuation of the tonal element resulted in greater disruption of extinction trials than the light in the adults, although the pups had faster, but nondifferential, extinction rates. A 3rd experiment presented 36 pups and adults with either 0-, 5-, or 10-sec delays of tonal CS reactivation after extinction responses. Both 5- and 10-sec delays resulted in increased numbers of extinction trials in the adults, but the pups failed to respond differentially to any of the delay intervals. Results of all of the experiments were considered in light of species-specific behaviors in avoidance extinction relative to environmental and associative saliencies between ages.", "contents": "Differential extinction of 2-way active avoidance in young and adult rats. Three experiments examined age-related differences in extinction of active avoidance in rats through manipulation of the stimulus context. In Experiment I, 36 weanlings and 36 adults acquired comparable avoidance levels to a tonal conditioned stimulus (CS), and novel stimuli consisting of either the presence of a naive rat or a light were presented during 2 tests in extinction. Results indicated more pronounced disruption of extinction in the pups, compared to adults, with the animate novel stimulus having the greater effect. Eighteen pups of 22 days of age and 18 adults in Experiment II were trained to avoid shock with a CS consisting of simultaneous tone and light onset. During extinction responses did not terminate 1 of the CS elements, but rather produced continuation of either the tone or light for an additional 5 sec. Continuation of the tonal element resulted in greater disruption of extinction trials than the light in the adults, although the pups had faster, but nondifferential, extinction rates. A 3rd experiment presented 36 pups and adults with either 0-, 5-, or 10-sec delays of tonal CS reactivation after extinction responses. Both 5- and 10-sec delays resulted in increased numbers of extinction trials in the adults, but the pups failed to respond differentially to any of the delay intervals. Results of all of the experiments were considered in light of species-specific behaviors in avoidance extinction relative to environmental and associative saliencies between ages."}
{"id": "PMID:478176", "title": "The effects of preparturient avoidance conditioning on postnatal caretaker behavior and offspring catecholamine levels and behavior in C57BL/6J mice.", "content": "Avoidance conditioning during pregnancy, changes in caretaker behavior during lactation, and changes in offspring catecholamine levels and behavior were investigated in C57BL/6J mice. Three equal groups of mice were employed. One group was avoidance conditioned prior to pregnancy and continued to avoid shock during pregnancy; a 2nd group was avoidance conditioned during pregnancy; and the 3rd group served as untreated controls. Offspring were crossfostered after birth. During lactation the proportion of time the caretaker spent on the nest was observed. At weaning pups were individually housed. Offspring dopamine concentrations were measured in the corpus striatum at birth and at days 22 and 42 of age. Their performance was evaluated in the open field at Day 42 of age. The results showed that mice receiving avoidance conditioning prior to parturition were more likely to cannibalize their offspring at parturition than were control animals. Furthermore, preparturiently avoidance conditioned mice spent less time on the nest if they reared offspring from biological mothers who had been avoidance conditioned when compared to similarly treated caretakers who reared offspring from untreated control biological mothers. Regardless of postnatal experiences, adult offspring from avoidance conditioned biological mothers had significantly reduced levels of dopamine in tissue from the corpus striatum and were significantly less active in the open field when compared with offspring from control biological mothers. Behavioral results support the findings of others that the prenatal environment plays an important role in shaping the later behavior of offspring. The decrease in dopamine levels suggests that avoidance training as one form of psychosocial stimulation during pregnancy can be disruptive to the development of the dopamine regulatory mechanisms.", "contents": "The effects of preparturient avoidance conditioning on postnatal caretaker behavior and offspring catecholamine levels and behavior in C57BL/6J mice. Avoidance conditioning during pregnancy, changes in caretaker behavior during lactation, and changes in offspring catecholamine levels and behavior were investigated in C57BL/6J mice. Three equal groups of mice were employed. One group was avoidance conditioned prior to pregnancy and continued to avoid shock during pregnancy; a 2nd group was avoidance conditioned during pregnancy; and the 3rd group served as untreated controls. Offspring were crossfostered after birth. During lactation the proportion of time the caretaker spent on the nest was observed. At weaning pups were individually housed. Offspring dopamine concentrations were measured in the corpus striatum at birth and at days 22 and 42 of age. Their performance was evaluated in the open field at Day 42 of age. The results showed that mice receiving avoidance conditioning prior to parturition were more likely to cannibalize their offspring at parturition than were control animals. Furthermore, preparturiently avoidance conditioned mice spent less time on the nest if they reared offspring from biological mothers who had been avoidance conditioned when compared to similarly treated caretakers who reared offspring from untreated control biological mothers. Regardless of postnatal experiences, adult offspring from avoidance conditioned biological mothers had significantly reduced levels of dopamine in tissue from the corpus striatum and were significantly less active in the open field when compared with offspring from control biological mothers. Behavioral results support the findings of others that the prenatal environment plays an important role in shaping the later behavior of offspring. The decrease in dopamine levels suggests that avoidance training as one form of psychosocial stimulation during pregnancy can be disruptive to the development of the dopamine regulatory mechanisms."}
{"id": "PMID:478177", "title": "Neonatal treatment of hamsters with barbiturate alters adult sexual behavior.", "content": "Male and female hamsters were given 50 micrograms pentobarbital, 100 micrograms pentobarbital, or 100 micrograms d-amphetamine on postnatal Days 2-4. When tested for masculine sexual behavior in adulthood, males treated with 100 micrograms pentobarbital showed behavioral deficits when tested with testes intact as well as after castration and treatment with testosterone propionate. Deficits shown by 50 micrograms pentobarbital males were overcome by testosterone replacement. When tested for feminine sexual behavior, males treated with 50 micrograms pentobarbital showed enhanced lordotic responses whereas males treated with 100 micrograms d-amphetamine showed no differences from controls when tested for female sexual behavior or when tested for male sexual behavior. Drug treatments had no effect on adult masculine or feminine sexual behavior in neonatally treated females. The results of this study show that pentobarbital can inhibit normal masculinization of the male when given during behavioral sexual differentiation.", "contents": "Neonatal treatment of hamsters with barbiturate alters adult sexual behavior. Male and female hamsters were given 50 micrograms pentobarbital, 100 micrograms pentobarbital, or 100 micrograms d-amphetamine on postnatal Days 2-4. When tested for masculine sexual behavior in adulthood, males treated with 100 micrograms pentobarbital showed behavioral deficits when tested with testes intact as well as after castration and treatment with testosterone propionate. Deficits shown by 50 micrograms pentobarbital males were overcome by testosterone replacement. When tested for feminine sexual behavior, males treated with 50 micrograms pentobarbital showed enhanced lordotic responses whereas males treated with 100 micrograms d-amphetamine showed no differences from controls when tested for female sexual behavior or when tested for male sexual behavior. Drug treatments had no effect on adult masculine or feminine sexual behavior in neonatally treated females. The results of this study show that pentobarbital can inhibit normal masculinization of the male when given during behavioral sexual differentiation."}
{"id": "PMID:478178", "title": "Early selective visual experience and pattern discrimination in hooded rats.", "content": "The early visual experience of hooded rats was restricted to either vertical or horizontal stripes. In a discrimination task pairing a gray surface and stripes of either the same orientation or an orientation orthogonal to that experienced during rearing, the rats made significantly fewer correct choices with the orthogonal orientation. However, the relatively lower overall performance of the vertically reared-horizontally tested animals was a major factor in the main effect of testing condition. We conclude that functional of the rat visual system through early selective visual experience is possible.", "contents": "Early selective visual experience and pattern discrimination in hooded rats. The early visual experience of hooded rats was restricted to either vertical or horizontal stripes. In a discrimination task pairing a gray surface and stripes of either the same orientation or an orientation orthogonal to that experienced during rearing, the rats made significantly fewer correct choices with the orthogonal orientation. However, the relatively lower overall performance of the vertically reared-horizontally tested animals was a major factor in the main effect of testing condition. We conclude that functional of the rat visual system through early selective visual experience is possible."}
{"id": "PMID:478179", "title": "Contrast effects in the rat: a developmental study.", "content": "Two experiments investigated and development of positive and negative contrast effects in the consummatory behavior of juvenile and adult rats. Over a 4-day session, animals were given alternating 30-sec presentations of 2 bottles containing either 32 or 4% sucrose solutions. In Experiment I, different groups of rats were tested both cross-sectionally and longitudinally using a cohort-sequential design. Cross-sectionally, animals at all ages exhibited both types of contrast and demonstrated increasing positive contrast effects with greater age. Discrepancies between the longitudinal and cross-sectional samples were found to be due to the repeated testing procedure of the former design. Experiment II replicated the positive and negative contrast effects in the juvenile animals, indicating that the processes responsible for contrast effects are operating in the rat at a very early age.", "contents": "Contrast effects in the rat: a developmental study. Two experiments investigated and development of positive and negative contrast effects in the consummatory behavior of juvenile and adult rats. Over a 4-day session, animals were given alternating 30-sec presentations of 2 bottles containing either 32 or 4% sucrose solutions. In Experiment I, different groups of rats were tested both cross-sectionally and longitudinally using a cohort-sequential design. Cross-sectionally, animals at all ages exhibited both types of contrast and demonstrated increasing positive contrast effects with greater age. Discrepancies between the longitudinal and cross-sectional samples were found to be due to the repeated testing procedure of the former design. Experiment II replicated the positive and negative contrast effects in the juvenile animals, indicating that the processes responsible for contrast effects are operating in the rat at a very early age."}
{"id": "PMID:478181", "title": "Counterregulatory hormonal responses to rapid glucose lowering in diabetic man.", "content": "To define whether rapid rate of fall in blood glucose stimulates counterregulatory hormonal responses in diabetic man, blood glucose in eight hyperglycemic diabetic subjects was rapidly lowered by intravenous insulin administration. Despite precipitous declines in blood glucose, plasma epinephrine and growth hormone remained virtually unchanged. In contrast, norepinephrine and cortisol increased significantly (P less than 0.025) in the face of hyperglycemia or euglycemia, while glucagon was suppressed (P less than 0.025). A transient modest fall in mean arterial pressure and a rise in pulse rate were noted. No correlation was observed between glucose disappearance rate or decrement in glucose concentration and the hormonal responses. After sham insulin administration, no change was observed in plasma epinephrine, norepinephrine, and cortisol levels. These findings suggest that rate of fall in blood glucose per se is not a primary signal for counterregulatory hormonal response. Cortisol but not growth hormone release during falling blood glucose in diabetic subjects can occur despite elevated blood glucose levels. The etiology of norepinephrine and cortisol change is unclear.", "contents": "Counterregulatory hormonal responses to rapid glucose lowering in diabetic man. To define whether rapid rate of fall in blood glucose stimulates counterregulatory hormonal responses in diabetic man, blood glucose in eight hyperglycemic diabetic subjects was rapidly lowered by intravenous insulin administration. Despite precipitous declines in blood glucose, plasma epinephrine and growth hormone remained virtually unchanged. In contrast, norepinephrine and cortisol increased significantly (P less than 0.025) in the face of hyperglycemia or euglycemia, while glucagon was suppressed (P less than 0.025). A transient modest fall in mean arterial pressure and a rise in pulse rate were noted. No correlation was observed between glucose disappearance rate or decrement in glucose concentration and the hormonal responses. After sham insulin administration, no change was observed in plasma epinephrine, norepinephrine, and cortisol levels. These findings suggest that rate of fall in blood glucose per se is not a primary signal for counterregulatory hormonal response. Cortisol but not growth hormone release during falling blood glucose in diabetic subjects can occur despite elevated blood glucose levels. The etiology of norepinephrine and cortisol change is unclear."}
{"id": "PMID:478182", "title": "In vitro studies of the substrates for energy production and the effects of insulin on glucose utilization in the neural components of peripheral nerve.", "content": "An \"endoneurial\" preparation from a rabbit tibial nerve fascicle was used to study the ability of peripheral nerve axons and Schwann cells to derive their composite energy requirements from glucose, D-beta-hydroxybutyrate, or albumin-bound palmitate, and the effects of insulin in vitro on their composite glucose utilization. Samples incubated with 5 mM glucose for 2 h maintained a stable O2 uptake and P-creatine and ATP concentrations, and they exhibited a slight increase in P-creatine/creatine ratio (the electron microscopic appearance of the preparation was previously shown to be unaltered under these conditions). The rate of glucose oxidation required to account for the O2 uptake accounted for 61% of the glucose uptake. In samples incubated without substrate for 2 h, a marked fall in tissue glucose was associated with a 50% decrease in O2 uptake and with decreases in P-creatine, ATP, and in the P-creatine/creating ratio. In medium lacking glucose but containing 5 mM DL-beta-hydroxybutyrate, a stable rate of D-beta-hydroxybutyrate uptake was observed, and acetoacetate production accounted for only a small fraction; significant decreases in O2 uptake or ATP were prevented, and, although P-creatinde and the P-creatine/creatine ratio fell, they remained significantly higher than after incubation without substrate. An efficient blood-nerve barrier to albumin is known to exist. Medium containing albumin-bound palmitate with molar ratios or palmitate/albumin of 1 or 2 (highest FFA concentration, 1.32 meq/L) failed to prevent decreases in P-creatine, ATP, and in the P-creatine/creatine ratio during incubations without glucose; the associated O2 uptakes suggested that the tissue is susceptible to respiratory uncoupling and depression son exposure to albumin-blund palmitate as compared with non-neural tissue. Insulin (100 or 1000 microU/ml) had no detectable effects on glucose utilization in the endoneurial preparation during 2-h incubations with 5 mM glucose or (U-14C) glucose. In contrast, in epineurial tissue from rabbit sciatic nerve, insulin (100 micronU/ml) increased (U-14C) glucose incorporation into CO2 and total lipid. The neural components of peripheral nerve are probably dependent on glucose as their major substrate for energy production and respiration under most physiologic conditions in which elevated plasma ketone body concentrations are absent; their composite glucose utilization is not subject to acute, direct regulation by insulin in concentrations that might reasonably be derived from plasma insulin of pancreatic origin.", "contents": "In vitro studies of the substrates for energy production and the effects of insulin on glucose utilization in the neural components of peripheral nerve. An \"endoneurial\" preparation from a rabbit tibial nerve fascicle was used to study the ability of peripheral nerve axons and Schwann cells to derive their composite energy requirements from glucose, D-beta-hydroxybutyrate, or albumin-bound palmitate, and the effects of insulin in vitro on their composite glucose utilization. Samples incubated with 5 mM glucose for 2 h maintained a stable O2 uptake and P-creatine and ATP concentrations, and they exhibited a slight increase in P-creatine/creatine ratio (the electron microscopic appearance of the preparation was previously shown to be unaltered under these conditions). The rate of glucose oxidation required to account for the O2 uptake accounted for 61% of the glucose uptake. In samples incubated without substrate for 2 h, a marked fall in tissue glucose was associated with a 50% decrease in O2 uptake and with decreases in P-creatine, ATP, and in the P-creatine/creating ratio. In medium lacking glucose but containing 5 mM DL-beta-hydroxybutyrate, a stable rate of D-beta-hydroxybutyrate uptake was observed, and acetoacetate production accounted for only a small fraction; significant decreases in O2 uptake or ATP were prevented, and, although P-creatinde and the P-creatine/creatine ratio fell, they remained significantly higher than after incubation without substrate. An efficient blood-nerve barrier to albumin is known to exist. Medium containing albumin-bound palmitate with molar ratios or palmitate/albumin of 1 or 2 (highest FFA concentration, 1.32 meq/L) failed to prevent decreases in P-creatine, ATP, and in the P-creatine/creatine ratio during incubations without glucose; the associated O2 uptakes suggested that the tissue is susceptible to respiratory uncoupling and depression son exposure to albumin-blund palmitate as compared with non-neural tissue. Insulin (100 or 1000 microU/ml) had no detectable effects on glucose utilization in the endoneurial preparation during 2-h incubations with 5 mM glucose or (U-14C) glucose. In contrast, in epineurial tissue from rabbit sciatic nerve, insulin (100 micronU/ml) increased (U-14C) glucose incorporation into CO2 and total lipid. The neural components of peripheral nerve are probably dependent on glucose as their major substrate for energy production and respiration under most physiologic conditions in which elevated plasma ketone body concentrations are absent; their composite glucose utilization is not subject to acute, direct regulation by insulin in concentrations that might reasonably be derived from plasma insulin of pancreatic origin."}
{"id": "PMID:478183", "title": "Reduction in portal vein blood flow by somatostatin.", "content": "Intravenous somatostatin boluses produced striking diminution in portal vein blood flow in dogs (maximally 40--55%). This effect was of rapid onset, without observable changes in hepatic artery flow, systemic blood pressure, pulse or central venous pressure. The duration of action was short, but could be sustained by continuous intravenous infusion. These observations are consistent with an endocrine role of somatostatin in the regulation of nutrient balance.", "contents": "Reduction in portal vein blood flow by somatostatin. Intravenous somatostatin boluses produced striking diminution in portal vein blood flow in dogs (maximally 40--55%). This effect was of rapid onset, without observable changes in hepatic artery flow, systemic blood pressure, pulse or central venous pressure. The duration of action was short, but could be sustained by continuous intravenous infusion. These observations are consistent with an endocrine role of somatostatin in the regulation of nutrient balance."}
{"id": "PMID:478185", "title": "High prevalence of diabetes in young Pima Indians: evidence of phenotypic variation in a genetically isolated population.", "content": "Medical records of the Pima Indian population aged 0--24 yr were reviewed for a diagnosis of diabetes before initiation of glucose tolerance testing. None of 1556 subjects below age 15, but 6 of 657 aged 15--24, had a previous diagnosis. Of the six known diabetics, five had been treated with insulin and four had had ketoacidosis. Subsequently, plasma glucose levels were determined after a 75-g oral carbohydrate load in 1712 subjects aged 5--24 yr, which is about 78% of the eligible population. Previously diagnosed diabetes and asymptomatic hyperglycemia were more frequent in subjects 15--24 yr old than were reported in other populations. Glucose intolerance in young Pimas was associated with obesity. In Pima offspring, the presence of diabetes in both parents was related to glucose tolerance in those above but not below the age of 15 yr. Both asymptomatic hyperglycemia and insulin-requiring diabetes occurred frequently in young Pimas, suggesting that these syndromes represent the clinical spectrum of a single disease in the Pima Indian.", "contents": "High prevalence of diabetes in young Pima Indians: evidence of phenotypic variation in a genetically isolated population. Medical records of the Pima Indian population aged 0--24 yr were reviewed for a diagnosis of diabetes before initiation of glucose tolerance testing. None of 1556 subjects below age 15, but 6 of 657 aged 15--24, had a previous diagnosis. Of the six known diabetics, five had been treated with insulin and four had had ketoacidosis. Subsequently, plasma glucose levels were determined after a 75-g oral carbohydrate load in 1712 subjects aged 5--24 yr, which is about 78% of the eligible population. Previously diagnosed diabetes and asymptomatic hyperglycemia were more frequent in subjects 15--24 yr old than were reported in other populations. Glucose intolerance in young Pimas was associated with obesity. In Pima offspring, the presence of diabetes in both parents was related to glucose tolerance in those above but not below the age of 15 yr. Both asymptomatic hyperglycemia and insulin-requiring diabetes occurred frequently in young Pimas, suggesting that these syndromes represent the clinical spectrum of a single disease in the Pima Indian."}
{"id": "PMID:478186", "title": "Genetic susceptibility to diabetes mellitus: the distribution of properdin factor B (Bf) and glyoxalase (GLO) phenotypes.", "content": "The distribution of phenotypes controlled by two loci on chromosome 6 has been studied in a series of 239 patients with type 1 (insulin-dependent) and 297 patients with type 2 (non-insulin-dependent) diabetes mellitus. At the properdin factor B (Bf) locus there is a significant increase in the frequency of the BfSu and BfF1 alleles for type 1 patients, and the combined inc;rease in frequency of BfS1 and BfF1 in those patients is highly significant. The relative risk for F1 is 6.2 and for F1 and S1 combined is 5.3. These results confirm the association with F1 reported recently by Raum and co-workers in Boston. The two rare alleles BfS1 and BfF1 are in significant negative disequilibrium with HLA B8. For the glyoxalase (GLO) locus there is a slight but nonsignificant increase in the frequency of the GLO2 allele, but a significant disturbance in the distribution of the GLO phenotypes for type 2 patients. These results for the GLO alleles may be due to stratification in our series of type 2 patients. Further studies are in progress to test this hypothesis.", "contents": "Genetic susceptibility to diabetes mellitus: the distribution of properdin factor B (Bf) and glyoxalase (GLO) phenotypes. The distribution of phenotypes controlled by two loci on chromosome 6 has been studied in a series of 239 patients with type 1 (insulin-dependent) and 297 patients with type 2 (non-insulin-dependent) diabetes mellitus. At the properdin factor B (Bf) locus there is a significant increase in the frequency of the BfSu and BfF1 alleles for type 1 patients, and the combined inc;rease in frequency of BfS1 and BfF1 in those patients is highly significant. The relative risk for F1 is 6.2 and for F1 and S1 combined is 5.3. These results confirm the association with F1 reported recently by Raum and co-workers in Boston. The two rare alleles BfS1 and BfF1 are in significant negative disequilibrium with HLA B8. For the glyoxalase (GLO) locus there is a slight but nonsignificant increase in the frequency of the GLO2 allele, but a significant disturbance in the distribution of the GLO phenotypes for type 2 patients. These results for the GLO alleles may be due to stratification in our series of type 2 patients. Further studies are in progress to test this hypothesis."}
{"id": "PMID:478187", "title": "Somatomedin activity and diabetic control in children with insulin-dependent diabetes.", "content": "To test the hypothesis that somatomedin activity is influenced by diabetes and its metabolic regulation, the relationship between somatomedin activity and diabetic control as assessed by hemoglobin A1C was investigated in 40 children with insulin-dependent diabetes. An inverse correlation between hemoglobin A1C and serum somatomedin activity was statistically significant. The data suggest that abnormalities of linear growth, which can occur in children with poorly controlled diabetes, may involve abnormalities in net somatomedin activity.", "contents": "Somatomedin activity and diabetic control in children with insulin-dependent diabetes. To test the hypothesis that somatomedin activity is influenced by diabetes and its metabolic regulation, the relationship between somatomedin activity and diabetic control as assessed by hemoglobin A1C was investigated in 40 children with insulin-dependent diabetes. An inverse correlation between hemoglobin A1C and serum somatomedin activity was statistically significant. The data suggest that abnormalities of linear growth, which can occur in children with poorly controlled diabetes, may involve abnormalities in net somatomedin activity."}
{"id": "PMID:478188", "title": "A multicenter inquiry into the etiology of pancreatic diseases.", "content": "A multicenter study on the etiology and diet of patients with pancreatic diseases has been realized with the collaboration of 36 centers in 19 countries having widely different climatic and racial conditions. 2,478 cases were studied: acute pancreatitis (AP), 222 males, 208 females; calcified chronic pancreatitis (CCP), 801 males, 134 females; non-calcified chronic pancreatitis (NCCP), 525 males, 155 females; pancreatic cancer (PK), 69 males, 14 females; controls, 281 males, 62 females. The analysis of mutual information and the factorial analysis of correspondences have been used. With regard to chronic pancreatitis, the 19 countries could be classified into 4 classes presenting relative similarities. (A) Southern Europe: The diet is rich in carbohydrates, protein and lipids, alcohol intake is primarily in the form of wine and the pathology is dominated by CCP. There are much fewer women than men with chronic pancreatitis. (B) Northern Europe, to which may be added Argentina and Chile, is characterized by a protein- and lipid-rich diet, a beer-based alcohol consumption and a distinct prevalence of AP and NCCP. The prevalence of males with chronic pancreatitis is less marked than in southern Europe. (C) Japan has a lipid-poor diet and a low frequency of CCP and NCCP. (D) A fourth group is mostly composed of tropical countries with mixed races. It may be divided into 2 subclasses: (a) India is the most characteristic country of the first type with low fat, low protein diet, no alcoholism, high frequency of CCP (at an early age); (b) Brasil and South Africa are representative of the second subclass with very high alcohol intake in the form of spirits and a high frequency of CCP.", "contents": "A multicenter inquiry into the etiology of pancreatic diseases. A multicenter study on the etiology and diet of patients with pancreatic diseases has been realized with the collaboration of 36 centers in 19 countries having widely different climatic and racial conditions. 2,478 cases were studied: acute pancreatitis (AP), 222 males, 208 females; calcified chronic pancreatitis (CCP), 801 males, 134 females; non-calcified chronic pancreatitis (NCCP), 525 males, 155 females; pancreatic cancer (PK), 69 males, 14 females; controls, 281 males, 62 females. The analysis of mutual information and the factorial analysis of correspondences have been used. With regard to chronic pancreatitis, the 19 countries could be classified into 4 classes presenting relative similarities. (A) Southern Europe: The diet is rich in carbohydrates, protein and lipids, alcohol intake is primarily in the form of wine and the pathology is dominated by CCP. There are much fewer women than men with chronic pancreatitis. (B) Northern Europe, to which may be added Argentina and Chile, is characterized by a protein- and lipid-rich diet, a beer-based alcohol consumption and a distinct prevalence of AP and NCCP. The prevalence of males with chronic pancreatitis is less marked than in southern Europe. (C) Japan has a lipid-poor diet and a low frequency of CCP and NCCP. (D) A fourth group is mostly composed of tropical countries with mixed races. It may be divided into 2 subclasses: (a) India is the most characteristic country of the first type with low fat, low protein diet, no alcoholism, high frequency of CCP (at an early age); (b) Brasil and South Africa are representative of the second subclass with very high alcohol intake in the form of spirits and a high frequency of CCP."}
{"id": "PMID:478189", "title": "Differences in serum bile acid composition between unoperated cirrhotic patients and patients with portacaval or mesocaval shunt.", "content": "Serum bile acids were determined in 13 cirrhotic patients with portacaval shunt and in 15 with mesocaval shunt. 20 nonshunted cirrhotics were used as controls. Serum bile acid levels were higher in portacaval (26.7 +/- 14.3 micrograms/ml) than in mesocaval shunt patients (16.9 +/- 8.1 micrograms/ml) and controls (14.1 +/- 11.5 micrograms/ml). The cholic/chenodeoxycholic ratio was lower after portacaval shunt than after mesocaval shunt (0.36 +/- 0.15 and 0.63 +/- 0.26, respectively). It is hypothesized that these findings may be consistent with a larger hepatic blood flow present in mesocaval shunted patients and with chenodeoxycholic absorption in the upper small intestine.", "contents": "Differences in serum bile acid composition between unoperated cirrhotic patients and patients with portacaval or mesocaval shunt. Serum bile acids were determined in 13 cirrhotic patients with portacaval shunt and in 15 with mesocaval shunt. 20 nonshunted cirrhotics were used as controls. Serum bile acid levels were higher in portacaval (26.7 +/- 14.3 micrograms/ml) than in mesocaval shunt patients (16.9 +/- 8.1 micrograms/ml) and controls (14.1 +/- 11.5 micrograms/ml). The cholic/chenodeoxycholic ratio was lower after portacaval shunt than after mesocaval shunt (0.36 +/- 0.15 and 0.63 +/- 0.26, respectively). It is hypothesized that these findings may be consistent with a larger hepatic blood flow present in mesocaval shunted patients and with chenodeoxycholic absorption in the upper small intestine."}
{"id": "PMID:478190", "title": "Radiation pancreatitis: a clinical entity?", "content": "2 cases of pancreatic disease in patients with malabsorption following small bowel radiation injury are reported. It is suggested that the pancreatic disease present in these patients occurred as a result of previous radiotherapy.", "contents": "Radiation pancreatitis: a clinical entity? 2 cases of pancreatic disease in patients with malabsorption following small bowel radiation injury are reported. It is suggested that the pancreatic disease present in these patients occurred as a result of previous radiotherapy."}
{"id": "PMID:478191", "title": "Effect of a feeding schedule on crypt cell proliferation in the small intestine of the rat.", "content": "Using a stathmokinetic technique, the mitotic rate in the small intestinal crypts of two groups of male Sprague-Dawley rats was studied. The first group was fed solids for 3 h daily only. The second group was fed solids ad libitum. In the first group it was found that the mean daily weight of food consumed rose quickly to normal 24-hour levels but the mean daily weight of the rats fell rapidly and did not approach the original level throughout the experiment. There was no significant difference in the mitotic rate of the small intestinal crypt cells between the two groups.", "contents": "Effect of a feeding schedule on crypt cell proliferation in the small intestine of the rat. Using a stathmokinetic technique, the mitotic rate in the small intestinal crypts of two groups of male Sprague-Dawley rats was studied. The first group was fed solids for 3 h daily only. The second group was fed solids ad libitum. In the first group it was found that the mean daily weight of food consumed rose quickly to normal 24-hour levels but the mean daily weight of the rats fell rapidly and did not approach the original level throughout the experiment. There was no significant difference in the mitotic rate of the small intestinal crypt cells between the two groups."}
{"id": "PMID:478192", "title": "Chronic administration of pentagastrin. Effects on pancreatic protein and nucleic acid contents and protein synthesis in rats.", "content": "Groups of rats were injected with either saline or pentagastrin (0.5, 1 or 2 mg/kg/day) for 14 days. Pancreatic weight, DNA, RNA and protein contents as well as the ability of the pancreatic polyribosomes to synthesize protein in a cell-free system were investigated. In all three pentagastrin-treated groups the weight of the pancreas was found to be significantly higher (33-73%) than in the control. DNA, RNA and protein contents were significantly increased by 47, 98 and 85%, respectively, in the group that received the highest dose of the hormone. With respect to ribosomal protein synthesis, while the pentagastrin dose of 1 and 2 mg/kg/day resulted in 25 and 72% augmentation, respectively, the dose of 0.5 mg/kg had no influence on protein synthesis. It is concluded that chronic administration of pentagastrin (2 mg/kg/day for 14 days) causes hyperplasia and hypertrophy of the pancreas.", "contents": "Chronic administration of pentagastrin. Effects on pancreatic protein and nucleic acid contents and protein synthesis in rats. Groups of rats were injected with either saline or pentagastrin (0.5, 1 or 2 mg/kg/day) for 14 days. Pancreatic weight, DNA, RNA and protein contents as well as the ability of the pancreatic polyribosomes to synthesize protein in a cell-free system were investigated. In all three pentagastrin-treated groups the weight of the pancreas was found to be significantly higher (33-73%) than in the control. DNA, RNA and protein contents were significantly increased by 47, 98 and 85%, respectively, in the group that received the highest dose of the hormone. With respect to ribosomal protein synthesis, while the pentagastrin dose of 1 and 2 mg/kg/day resulted in 25 and 72% augmentation, respectively, the dose of 0.5 mg/kg had no influence on protein synthesis. It is concluded that chronic administration of pentagastrin (2 mg/kg/day for 14 days) causes hyperplasia and hypertrophy of the pancreas."}
{"id": "PMID:478194", "title": "Prolonged manometric study of the gastroduodenal junction in man.", "content": "A study of the motor activity of the gastroduodenal (GD) junction has been carried out on 8 subjects by using an original probe provided with a suction cup, that allows not only a duodenogastric (DG) pull-though, but also a prolonged recording of the pyloric, antral and duodenal motor activity, in basal condition and after intraduodenal instillation of HCl 0.1 N and intravenous infusion of cerulein. The position of the probe was controlled with fluoroscopy and transmural potential difference. The DG pull-through did not show a zone of high pressure at the GD junction, but the prolonged study showed, during HC1 and cerulein administrations, a significant increase of the pyloric tonus.", "contents": "Prolonged manometric study of the gastroduodenal junction in man. A study of the motor activity of the gastroduodenal (GD) junction has been carried out on 8 subjects by using an original probe provided with a suction cup, that allows not only a duodenogastric (DG) pull-though, but also a prolonged recording of the pyloric, antral and duodenal motor activity, in basal condition and after intraduodenal instillation of HCl 0.1 N and intravenous infusion of cerulein. The position of the probe was controlled with fluoroscopy and transmural potential difference. The DG pull-through did not show a zone of high pressure at the GD junction, but the prolonged study showed, during HC1 and cerulein administrations, a significant increase of the pyloric tonus."}
{"id": "PMID:478195", "title": "Effect of somatostatin on normal and gastric-stimulated cell proliferation in the gastric and intestinal mucosae of the rat.", "content": "The effects of an 8-hour infusion of somatostatin, saline, gastrin or a mixture of gastrin + somatostatin on DNA synthesis and mitotic activity of gastrointestinal progenitor cells were explored in conscious rats, using in vivo labeling with 3H-thymidine and radioautography. Infusion of somatostatin (day or night) was shown to transiently reduce nuclear uptake of 3H-thymidine and cell division in both fundic and antral progenitor cells. Cell DNA synthesis in the gastrin + somatostatin-stimulated stomach was lower than in the gastrin-stimulated stomach. In the intestine, nocturnal infusion of somatostatin decrease DNA synthesis whereas diurnal infusion decreased cell division but no effect was observed on gastrin stimulation. Evidently, somatostatin may inhibit, perhaps indirectly, the cell proliferation in digestive mucosae and antagonize the trophic activity of gastrin but only in fundic and antral mucosae.", "contents": "Effect of somatostatin on normal and gastric-stimulated cell proliferation in the gastric and intestinal mucosae of the rat. The effects of an 8-hour infusion of somatostatin, saline, gastrin or a mixture of gastrin + somatostatin on DNA synthesis and mitotic activity of gastrointestinal progenitor cells were explored in conscious rats, using in vivo labeling with 3H-thymidine and radioautography. Infusion of somatostatin (day or night) was shown to transiently reduce nuclear uptake of 3H-thymidine and cell division in both fundic and antral progenitor cells. Cell DNA synthesis in the gastrin + somatostatin-stimulated stomach was lower than in the gastrin-stimulated stomach. In the intestine, nocturnal infusion of somatostatin decrease DNA synthesis whereas diurnal infusion decreased cell division but no effect was observed on gastrin stimulation. Evidently, somatostatin may inhibit, perhaps indirectly, the cell proliferation in digestive mucosae and antagonize the trophic activity of gastrin but only in fundic and antral mucosae."}
{"id": "PMID:478196", "title": "Gastrointestinal motility and absorption of calcium during infusion of somatostatin in the rat.", "content": "The effect of somatostatin on gastric evacuation, small bowel propulsion and gastrointestinal (GI) absorption of calcium was studied in conscious rats. Nonabsorbable 125I-PVP was deposited in the stomach or in the duodenum and the distribution of the radioactivity along the excised GI specimen was recorded after 15 min. 47Ca in chloride from was administered simultaneously and the amont of radioactivity remaining in the GI speciment at sacrifice could easily be measured. Intravenous infusion of synthetic somatostatin (50 micrograms.kg-1.h-1), which resulted in a manifest although not extreme hypersomatostatinemia had no effect either on gastric evacuation, small bowel propulsion or GI absorption of calcium. The findings do not indicate that somatostatin has a direct effect on GI motility or absorption of calcium.", "contents": "Gastrointestinal motility and absorption of calcium during infusion of somatostatin in the rat. The effect of somatostatin on gastric evacuation, small bowel propulsion and gastrointestinal (GI) absorption of calcium was studied in conscious rats. Nonabsorbable 125I-PVP was deposited in the stomach or in the duodenum and the distribution of the radioactivity along the excised GI specimen was recorded after 15 min. 47Ca in chloride from was administered simultaneously and the amont of radioactivity remaining in the GI speciment at sacrifice could easily be measured. Intravenous infusion of synthetic somatostatin (50 micrograms.kg-1.h-1), which resulted in a manifest although not extreme hypersomatostatinemia had no effect either on gastric evacuation, small bowel propulsion or GI absorption of calcium. The findings do not indicate that somatostatin has a direct effect on GI motility or absorption of calcium."}
{"id": "PMID:478197", "title": "Role of homocytotropic antibodies in pathogenesis of gastric ulcer.", "content": "Praomys (Mastomys) natalensis were sensitised by peritoneal injection of ovalbumin mixed with aluminium hydroxide. The animals developed homocytotoropic IgE and IgG 1 antibodies. When presensitised animals were challenged with ovalbumin in the gastric wall, a gastric ulcer appeared. This ulceration, induced by an immunological release of histamine, could be inhibited by the administration of disodium cromoglycate. The present model is useful for producing a gastric ulcer in a determined site. It supports the theory of a possible allergic component in the pathogenesis of the human disease.", "contents": "Role of homocytotropic antibodies in pathogenesis of gastric ulcer. Praomys (Mastomys) natalensis were sensitised by peritoneal injection of ovalbumin mixed with aluminium hydroxide. The animals developed homocytotoropic IgE and IgG 1 antibodies. When presensitised animals were challenged with ovalbumin in the gastric wall, a gastric ulcer appeared. This ulceration, induced by an immunological release of histamine, could be inhibited by the administration of disodium cromoglycate. The present model is useful for producing a gastric ulcer in a determined site. It supports the theory of a possible allergic component in the pathogenesis of the human disease."}
{"id": "PMID:478198", "title": "Study of parotid and mixed saliva in the diagnosis of chronic pancreatitis.", "content": "A study on the diagnostic value of the parotid and mixed saliva assay after stimulation with 1% pilocarpine hydrochloride was carried out in 36 controls and 26 patients affected with chronic pancreatitis. No statistical difference between the two groups was found as far as saliva volume, bicarbonate and amylase (concentration and output) are concerned. No correlation was found between the results of the saliva test and those of the secretin-pancreozymin test or endoscopic retrograde pancreatography. These data excluded any diagnostic role of the saliva test in chronic pancreatitis.", "contents": "Study of parotid and mixed saliva in the diagnosis of chronic pancreatitis. A study on the diagnostic value of the parotid and mixed saliva assay after stimulation with 1% pilocarpine hydrochloride was carried out in 36 controls and 26 patients affected with chronic pancreatitis. No statistical difference between the two groups was found as far as saliva volume, bicarbonate and amylase (concentration and output) are concerned. No correlation was found between the results of the saliva test and those of the secretin-pancreozymin test or endoscopic retrograde pancreatography. These data excluded any diagnostic role of the saliva test in chronic pancreatitis."}
{"id": "PMID:478199", "title": "Compartmental analysis of the plasma clearance of tetrabromsulphthalein and dibromsulphthalein in the normal dog and in patients with liver disease.", "content": "A comparison has been made of the plasma clearances of the anionic dye analogues, tetrabromsulphthalein (BSP) and dibromsulphthalein (dBSP), analysed by means of a two-compartmental model. In the normal conscious dog all three transport rate constants and initial and steady state plasma clearances were greater with dBSP than BSP. On the other hand the equivalent liver volume (ELV) and storage capacity were lower with dBSP. In patients with various hepatic disorders dBSP values were greater than those obtained with BSP apart from rate constant b (liver to plasma). Patients with advanced liver impairment in general exhibited lower hepatic uptake, clearance and storage of both dyes compared to patients with mild forms of liver disease. The ratio of ELV values for BSP and dBSP appeared to be related to overall hepatic function, with ratios greater than 1 in the normal dogs and less than 1 in the patients.", "contents": "Compartmental analysis of the plasma clearance of tetrabromsulphthalein and dibromsulphthalein in the normal dog and in patients with liver disease. A comparison has been made of the plasma clearances of the anionic dye analogues, tetrabromsulphthalein (BSP) and dibromsulphthalein (dBSP), analysed by means of a two-compartmental model. In the normal conscious dog all three transport rate constants and initial and steady state plasma clearances were greater with dBSP than BSP. On the other hand the equivalent liver volume (ELV) and storage capacity were lower with dBSP. In patients with various hepatic disorders dBSP values were greater than those obtained with BSP apart from rate constant b (liver to plasma). Patients with advanced liver impairment in general exhibited lower hepatic uptake, clearance and storage of both dyes compared to patients with mild forms of liver disease. The ratio of ELV values for BSP and dBSP appeared to be related to overall hepatic function, with ratios greater than 1 in the normal dogs and less than 1 in the patients."}
{"id": "PMID:478200", "title": "Plasmapheresis in Crohn's disease.", "content": "6 patients with Crohn's disease for 1-14 years have been treated with plasmapheresis for periods of up to 6 months. The symptoms of the one newly diagnosed patient were controlled by plasmapheresis alone, and 5 steroid-dependent patients were able to reduce their steroid requirements. During the study period, 13 clinical relapses were managed without increasing steroid dosage. There was evidence of circulating soluble immune complexes in all the patients and these levels were lowered by plasmapheresis. A possible role of plasmapheresis in the management of Crohn's disease is discussed.", "contents": "Plasmapheresis in Crohn's disease. 6 patients with Crohn's disease for 1-14 years have been treated with plasmapheresis for periods of up to 6 months. The symptoms of the one newly diagnosed patient were controlled by plasmapheresis alone, and 5 steroid-dependent patients were able to reduce their steroid requirements. During the study period, 13 clinical relapses were managed without increasing steroid dosage. There was evidence of circulating soluble immune complexes in all the patients and these levels were lowered by plasmapheresis. A possible role of plasmapheresis in the management of Crohn's disease is discussed."}
{"id": "PMID:478201", "title": "Etiology of hepatitis B surface antigen (HBsAg)-negative chronic hepatitis.", "content": "A study was undertaken to elucidate the etiology of HBsAg-negative chronic hepatitis. Form 37 individuals with HBsAg-negative chronic hepatitis, 11 had liver membrane autoantibody (LMA) and were thus classified as autoimmune. 6 patients had anti-HBc, 1 of which was also positive for LMA. The majority of individuals with HBsAg-negative chronic hepatitis had antibodies to hepatitis A antigen (anti-HAV), in general at low titer. We conclude from our data that hepatitis A and hepatitis B virus infections are unlikely to play a significant role in inducing or maintaining HBs-Ag-negative chronic hepatitis. The etiological role of non-A non-B hepatitis agent(s) is difficult to estimate and must await the detection of appropriate markers for type non-A non-B hepatitis.", "contents": "Etiology of hepatitis B surface antigen (HBsAg)-negative chronic hepatitis. A study was undertaken to elucidate the etiology of HBsAg-negative chronic hepatitis. Form 37 individuals with HBsAg-negative chronic hepatitis, 11 had liver membrane autoantibody (LMA) and were thus classified as autoimmune. 6 patients had anti-HBc, 1 of which was also positive for LMA. The majority of individuals with HBsAg-negative chronic hepatitis had antibodies to hepatitis A antigen (anti-HAV), in general at low titer. We conclude from our data that hepatitis A and hepatitis B virus infections are unlikely to play a significant role in inducing or maintaining HBs-Ag-negative chronic hepatitis. The etiological role of non-A non-B hepatitis agent(s) is difficult to estimate and must await the detection of appropriate markers for type non-A non-B hepatitis."}
{"id": "PMID:478202", "title": "Gastric emptying after vagotomy and pyloric ring stretch. A preliminary report.", "content": "Using a radioisotope technique, gastric emptyping of a solid meal was measured in 10 pre-operative duodenal ulcer (DU) patients, 10 post-operative truncal vagotomy and pyloroplasty (TV+P), and 8 post-operative truncal vagotomy and pyloric stretch (TV+S) patients. Results show that after the first 20 min, although gastric emptyping after TV+S shows a tendency to reduction in rate compared with TV+P, the difference never reaches statistical significance (p greater than 0.01). When compared with control DU patients, the TV+S group again exhibits a statistically insignificant (p greater than 0.10) tendency to reduction in rate after the first 20 min, whereas the TV+P group shows no such tendency. The significance of these findings is discussed in relation to the use of a pyloric ring stretch as an alternative to pyloroplasty.", "contents": "Gastric emptying after vagotomy and pyloric ring stretch. A preliminary report. Using a radioisotope technique, gastric emptyping of a solid meal was measured in 10 pre-operative duodenal ulcer (DU) patients, 10 post-operative truncal vagotomy and pyloroplasty (TV+P), and 8 post-operative truncal vagotomy and pyloric stretch (TV+S) patients. Results show that after the first 20 min, although gastric emptyping after TV+S shows a tendency to reduction in rate compared with TV+P, the difference never reaches statistical significance (p greater than 0.01). When compared with control DU patients, the TV+S group again exhibits a statistically insignificant (p greater than 0.10) tendency to reduction in rate after the first 20 min, whereas the TV+P group shows no such tendency. The significance of these findings is discussed in relation to the use of a pyloric ring stretch as an alternative to pyloroplasty."}
{"id": "PMID:478203", "title": "Incidence of large bowel cancer in C\u00f4te-d'Or (Burgundy).", "content": "The present study is based on the registry of digestive cancers set up for the French department of C\u00f4te-d'Or (455,727 residents). Large bowel cancer represents half of the gastrointestinal tract cancers recorded in the course of 2 years (1976-1977). The crude annual incidence rate was 52.2 per 100,000 for males, 41.7 per 100,000 for females. The age-standardized incidence rate for cancer of the rectum is one of the highest reported. The incidence rates for cancer of the colon are in the intermediate range. Half of the large bowel cancers were rectal cancers, nearly always adenocarcinoma. Coexisting benign polyps were seen in 21% of the cases at the time of diagnosis of carcinoma and were more common in males than in females. 54% of cancers of the colon and 62% of cancers of the rectum underwent curative surgery.", "contents": "Incidence of large bowel cancer in C\u00f4te-d'Or (Burgundy). The present study is based on the registry of digestive cancers set up for the French department of C\u00f4te-d'Or (455,727 residents). Large bowel cancer represents half of the gastrointestinal tract cancers recorded in the course of 2 years (1976-1977). The crude annual incidence rate was 52.2 per 100,000 for males, 41.7 per 100,000 for females. The age-standardized incidence rate for cancer of the rectum is one of the highest reported. The incidence rates for cancer of the colon are in the intermediate range. Half of the large bowel cancers were rectal cancers, nearly always adenocarcinoma. Coexisting benign polyps were seen in 21% of the cases at the time of diagnosis of carcinoma and were more common in males than in females. 54% of cancers of the colon and 62% of cancers of the rectum underwent curative surgery."}
{"id": "PMID:478204", "title": "Action of tiadenol and clofibrate on biliary excretions of cholesterol and bile salts in rats.", "content": "Biliary excretion of cholesterol, phospholipids and bile salts, and the excretion of labeled cholesterol injected intravenously were studied in rats treated for 1 or 3 weeks with the hypolipidemic drugs tiadenol and clofibrate. Total biliary cholesterol concentration and output decreased in treated rats, and the cholesterol solubility ratio increased. The excretion of labeled bile salts increased with both drugs; the excretion of labeled cholesterol increased with clofibrate and tended to decrease with tiadenol. The ratio of labeled bile salts/labeled cholesterol was higher with tiadenol than with clofibrate.", "contents": "Action of tiadenol and clofibrate on biliary excretions of cholesterol and bile salts in rats. Biliary excretion of cholesterol, phospholipids and bile salts, and the excretion of labeled cholesterol injected intravenously were studied in rats treated for 1 or 3 weeks with the hypolipidemic drugs tiadenol and clofibrate. Total biliary cholesterol concentration and output decreased in treated rats, and the cholesterol solubility ratio increased. The excretion of labeled bile salts increased with both drugs; the excretion of labeled cholesterol increased with clofibrate and tended to decrease with tiadenol. The ratio of labeled bile salts/labeled cholesterol was higher with tiadenol than with clofibrate."}
{"id": "PMID:478205", "title": "Antisecretory actions of aminophylline in the rat and dog.", "content": "The effect of aminophylline on gastric acid secretion has been studied in the rat and dog. Aminophylline was shown to possess antisecretory activity when given either by oral or parenteral administration in an acute gastric fistula rat preparation. In the chronic gastric fistula dog aminophylline at 50 mg/kg intragastrically induced a small but significant stimulation of basal acid secretion. This dose of aminophylline also significantly inhibited acid secretion induced by gastrin tetrapeptide, and 2-deoxy-D-glucose but did not block acid secretion induced by histamine.", "contents": "Antisecretory actions of aminophylline in the rat and dog. The effect of aminophylline on gastric acid secretion has been studied in the rat and dog. Aminophylline was shown to possess antisecretory activity when given either by oral or parenteral administration in an acute gastric fistula rat preparation. In the chronic gastric fistula dog aminophylline at 50 mg/kg intragastrically induced a small but significant stimulation of basal acid secretion. This dose of aminophylline also significantly inhibited acid secretion induced by gastrin tetrapeptide, and 2-deoxy-D-glucose but did not block acid secretion induced by histamine."}
{"id": "PMID:478206", "title": "Oral domperidone in chronic postprandial dyspepsia. A double-blind placebo-controlled evaluation.", "content": "32 patients with chronic postprandial dyspepsia were selected for a 4-week controlled double-blind trial of domperidone and a placebo. The patients received either domperidone or a placebo at a dose rate of two 10-mg tablets t.i.d. before meals. A questionnaire was completed at the start of the study, and after 2 and 4 weeks of treatment. Excellent or good improvement was obtained in 71% of the domperidone-treated patients compared to only 13% of the placebo-treated cases. Domperidone was also significantly superior to the placebo when both drugs were compared to previously used medications. Side effects were not reported. It is concluded that domperidone has a beneficial effect on chronic postprandial dyspepsia.", "contents": "Oral domperidone in chronic postprandial dyspepsia. A double-blind placebo-controlled evaluation. 32 patients with chronic postprandial dyspepsia were selected for a 4-week controlled double-blind trial of domperidone and a placebo. The patients received either domperidone or a placebo at a dose rate of two 10-mg tablets t.i.d. before meals. A questionnaire was completed at the start of the study, and after 2 and 4 weeks of treatment. Excellent or good improvement was obtained in 71% of the domperidone-treated patients compared to only 13% of the placebo-treated cases. Domperidone was also significantly superior to the placebo when both drugs were compared to previously used medications. Side effects were not reported. It is concluded that domperidone has a beneficial effect on chronic postprandial dyspepsia."}
{"id": "PMID:478207", "title": "Abnormalities of jejunal mucosal enzymes in ulcerative colitis and Crohn's disease.", "content": "Jejunal mucosal function and structure was examined in 31 patients with ulcerative colitis and 29 patients with Crohn's disease with ileal, ileocolonic or colonic involvement; A significant reduction of the specific activity of disaccharidases (lactase, sucrase and trehalase) in jejunal mucosal homogenate occurred in patients with inflammatory bowel disease. Similarly, alkaline phosphatase was reduced in ulcerative colitis. Several dipeptidases such as glycyl-leucine, leucyl-glycine, glycyl-glycine and valyl-proline hydrolase activities were lower in patients with inflammatory bowel disease than in controls. Histological changes in jejunal mucosal biopsies occurred in 71% of patients with ulcerative colitis and 61% with Crohn's disease. These changes ranged from mild abnormalities of villus architecture to marked reduction of villus height. Most patients with a reduction in mucosal enzymes had concommitant morphological changes in jejunal mucosal biopsy. The results of this study indicate that functional and structural abnormalities of the jejunal mucosa frequently occur in patients with inflammatory bowel disease without radiologic evidence of proximal small bowel involvement.", "contents": "Abnormalities of jejunal mucosal enzymes in ulcerative colitis and Crohn's disease. Jejunal mucosal function and structure was examined in 31 patients with ulcerative colitis and 29 patients with Crohn's disease with ileal, ileocolonic or colonic involvement; A significant reduction of the specific activity of disaccharidases (lactase, sucrase and trehalase) in jejunal mucosal homogenate occurred in patients with inflammatory bowel disease. Similarly, alkaline phosphatase was reduced in ulcerative colitis. Several dipeptidases such as glycyl-leucine, leucyl-glycine, glycyl-glycine and valyl-proline hydrolase activities were lower in patients with inflammatory bowel disease than in controls. Histological changes in jejunal mucosal biopsies occurred in 71% of patients with ulcerative colitis and 61% with Crohn's disease. These changes ranged from mild abnormalities of villus architecture to marked reduction of villus height. Most patients with a reduction in mucosal enzymes had concommitant morphological changes in jejunal mucosal biopsy. The results of this study indicate that functional and structural abnormalities of the jejunal mucosa frequently occur in patients with inflammatory bowel disease without radiologic evidence of proximal small bowel involvement."}
{"id": "PMID:478208", "title": "The cholyl glycine-1-14C breath test in various gastrointestinal disorders.", "content": "The cholyl glycine-1-14C breath test was evaluated in a variety of gastrointestinal disorders. 138 tests were performed in 106 patients. Methods of data expression were evaluated and the cumulative 8-hour value was used. In 27 control patients the upper limit of the normal was found to be 78. A good correlation was found between the peak values and the cumulative 8-hour values (r = 0.95, p less than 0.01). The reproducibility of the test was good (r = 0.985, p less than 0.05). Abnormal results were found in 12 out of 13 cases with resection of the ileum and 11 out of 14 cases with Crohn's disease of the distal small bowel. The test was normal in cases with diseases of the proximal small bowel (celiac, Whipple's and Chron's diseases). The test was also normal in patients with colitis. It was abnormal in some of the cases after cholecystectomy and in most cases with carcinoma of the pancreas. The breath test was useful in monitoring the results of treatment in bacterial overgrowth of the small bowel. False negative results were observed after antibiotic treatment. The method seems to be more sensitive than the Schilling test in diagnosing disease of the distal small bowel.", "contents": "The cholyl glycine-1-14C breath test in various gastrointestinal disorders. The cholyl glycine-1-14C breath test was evaluated in a variety of gastrointestinal disorders. 138 tests were performed in 106 patients. Methods of data expression were evaluated and the cumulative 8-hour value was used. In 27 control patients the upper limit of the normal was found to be 78. A good correlation was found between the peak values and the cumulative 8-hour values (r = 0.95, p less than 0.01). The reproducibility of the test was good (r = 0.985, p less than 0.05). Abnormal results were found in 12 out of 13 cases with resection of the ileum and 11 out of 14 cases with Crohn's disease of the distal small bowel. The test was normal in cases with diseases of the proximal small bowel (celiac, Whipple's and Chron's diseases). The test was also normal in patients with colitis. It was abnormal in some of the cases after cholecystectomy and in most cases with carcinoma of the pancreas. The breath test was useful in monitoring the results of treatment in bacterial overgrowth of the small bowel. False negative results were observed after antibiotic treatment. The method seems to be more sensitive than the Schilling test in diagnosing disease of the distal small bowel."}
{"id": "PMID:478209", "title": "Embryonic appearance of alpha, beta, and gamma crystallins in the periodic albinism (ap) mutant of Xenopus laevis.", "content": "The appearance of the crystallins during lens development in the periodic albinism (ap/ap) mutant of Xenopus laevis has been studied. Using antibodies specific for total crystallins, alpha + beta crystallins, and gamma crystallins in the immunofluorescence technique, the first positive reaction for all could be demonstrated in the Nieuwkoop-Faber Stage 31 lens rudiment. The antibody to alpha + beta crystallins exhibited differences in intensity from cell to cell in the early rudiment, while the reaction to the other antibodies was uniform throughout the rudiment. As lens differentiation progressed, immunofluorescence was restricted in all cases to the lens fiber area, up to and including Nieuwkas positive, however, for total lens crystallins. These results are at variance with earlier studies on lens development and the crystallins in wildtype (+/+) X. laevis, where a positive reaction for gamma and total crystallins could be detector total lens crystallins. That this divergence in the mutant is due to a pleiotropic effect or directly to the inductive failure of the endomesoderm to initiate melanogenesis, is discussed.", "contents": "Embryonic appearance of alpha, beta, and gamma crystallins in the periodic albinism (ap) mutant of Xenopus laevis. The appearance of the crystallins during lens development in the periodic albinism (ap/ap) mutant of Xenopus laevis has been studied. Using antibodies specific for total crystallins, alpha + beta crystallins, and gamma crystallins in the immunofluorescence technique, the first positive reaction for all could be demonstrated in the Nieuwkoop-Faber Stage 31 lens rudiment. The antibody to alpha + beta crystallins exhibited differences in intensity from cell to cell in the early rudiment, while the reaction to the other antibodies was uniform throughout the rudiment. As lens differentiation progressed, immunofluorescence was restricted in all cases to the lens fiber area, up to and including Nieuwkas positive, however, for total lens crystallins. These results are at variance with earlier studies on lens development and the crystallins in wildtype (+/+) X. laevis, where a positive reaction for gamma and total crystallins could be detector total lens crystallins. That this divergence in the mutant is due to a pleiotropic effect or directly to the inductive failure of the endomesoderm to initiate melanogenesis, is discussed."}
{"id": "PMID:478210", "title": "Developmental capacity of aneuploid Xenopus species hybrids.", "content": "Diploid as well as triploid Xenopus interspecific hybrids generate aneuploid eggs because of the presence, at meiosis, of univalent chromosomes which are presumably distributed at random. Zygotes obtained from such eggs, fertilized by either normal or UV-irradiated sperm, were analysed for their developmental capacities. All monosomics die in the course of embryogenesis, whereby optimum capacities correspond closely with those observed in monosomic mammalian embryos, especially in mice. In contrast, hyperdiploid Xenopus are relatively viable: although many die exhibiting the 'haploid syndrome' or various other abnormalities, 8% of them reach metamorphosis, and 1-2% become adults. Of the latter, the karyotype was established in 13 individuals. Among them, 8-16 supernumerary chromosomes were found to be present.", "contents": "Developmental capacity of aneuploid Xenopus species hybrids. Diploid as well as triploid Xenopus interspecific hybrids generate aneuploid eggs because of the presence, at meiosis, of univalent chromosomes which are presumably distributed at random. Zygotes obtained from such eggs, fertilized by either normal or UV-irradiated sperm, were analysed for their developmental capacities. All monosomics die in the course of embryogenesis, whereby optimum capacities correspond closely with those observed in monosomic mammalian embryos, especially in mice. In contrast, hyperdiploid Xenopus are relatively viable: although many die exhibiting the 'haploid syndrome' or various other abnormalities, 8% of them reach metamorphosis, and 1-2% become adults. Of the latter, the karyotype was established in 13 individuals. Among them, 8-16 supernumerary chromosomes were found to be present."}
{"id": "PMID:478211", "title": "Cell contacts between chorda-mesoderm and the overlaying neuroectoderm (presumptive central nervous system) during the period of primary embryonic induction in amphibians.", "content": "Using transmission and scanning electron microscopy we were able to show that during primary embryonic induction in amphibians (Triturus alpestris) the interspace between the inducing chorda-mesoderm and the reacting ectoderm (presumptive medullary plate) of mid-gastrula stages is traversed by cell projections starting from cells of both tissue layers. In addition intimate membrane contacts between the main bodies of the ectodermal and chorda-mesodermal cells could be observed. It could be ruled out that cytoplasmic bridges (anastomosis) exist between cells of inducing chorda-mesoderm and reacting ectoderm, which would allow a free transfer of inducing substances without passing through membranes, as Eakin and Lehmann [1] have postulated. The possible role of cell to cell contact for neural induction is emphasized.", "contents": "Cell contacts between chorda-mesoderm and the overlaying neuroectoderm (presumptive central nervous system) during the period of primary embryonic induction in amphibians. Using transmission and scanning electron microscopy we were able to show that during primary embryonic induction in amphibians (Triturus alpestris) the interspace between the inducing chorda-mesoderm and the reacting ectoderm (presumptive medullary plate) of mid-gastrula stages is traversed by cell projections starting from cells of both tissue layers. In addition intimate membrane contacts between the main bodies of the ectodermal and chorda-mesodermal cells could be observed. It could be ruled out that cytoplasmic bridges (anastomosis) exist between cells of inducing chorda-mesoderm and reacting ectoderm, which would allow a free transfer of inducing substances without passing through membranes, as Eakin and Lehmann [1] have postulated. The possible role of cell to cell contact for neural induction is emphasized."}
{"id": "PMID:478212", "title": "The synthesis of hyaluronic acid by ectoderm during early organogenesis in the chick embryo.", "content": "This study demonstrates that the dorsal ectoderm of the stage 14 chick embryo synthesizes hyaluronic acid. About 49 to 52% of the H3 glucosamine-labeled glycosaminoglycan that is synthesized by explanted ectoderm can be identified as hyaluronic acid on the basis of its susceptibility to Streptomyces hyaluronidase or isolation of chondroitinase ABC digestion products. In addition, autoradiographic evidence shows that the ectoderm, unlike adjacent tissues like epithelial somites or neural tube, incorporates glucosamine into hyaluronidase-sensitive material which becomes largely extracellular and localized in the subectodermal cell-free space. Ultrastructural evidence shows that there is a fine fibrillar matrix between the ectodermal cells and in the subectodermal spaces when tannic acid is included in the primary fixative. This material resembles authentic hyaluronate, similarly fixed, and is absent when tannic acid is omitted from the fixative or when embryos have been previously treated in ovo with Streptomyces hyaluronidase. The concomitant reduction in the intercellular and subectodermal cell-free spaces after in ovo treatment with Streptomyces hyaluronidase supports the hypothesis that the dorsal ectoderm plays a morphogenetic role by contributing hyaluronate to the forming extracellular spaces. It is proposed that ectodermally derived hyaluronate might influence the morphogenesis of subjacent tissues such as the dermatome and neural crest.", "contents": "The synthesis of hyaluronic acid by ectoderm during early organogenesis in the chick embryo. This study demonstrates that the dorsal ectoderm of the stage 14 chick embryo synthesizes hyaluronic acid. About 49 to 52% of the H3 glucosamine-labeled glycosaminoglycan that is synthesized by explanted ectoderm can be identified as hyaluronic acid on the basis of its susceptibility to Streptomyces hyaluronidase or isolation of chondroitinase ABC digestion products. In addition, autoradiographic evidence shows that the ectoderm, unlike adjacent tissues like epithelial somites or neural tube, incorporates glucosamine into hyaluronidase-sensitive material which becomes largely extracellular and localized in the subectodermal cell-free space. Ultrastructural evidence shows that there is a fine fibrillar matrix between the ectodermal cells and in the subectodermal spaces when tannic acid is included in the primary fixative. This material resembles authentic hyaluronate, similarly fixed, and is absent when tannic acid is omitted from the fixative or when embryos have been previously treated in ovo with Streptomyces hyaluronidase. The concomitant reduction in the intercellular and subectodermal cell-free spaces after in ovo treatment with Streptomyces hyaluronidase supports the hypothesis that the dorsal ectoderm plays a morphogenetic role by contributing hyaluronate to the forming extracellular spaces. It is proposed that ectodermally derived hyaluronate might influence the morphogenesis of subjacent tissues such as the dermatome and neural crest."}
{"id": "PMID:478213", "title": "Regeneration of vitamin A deficient rat tracheal epithelium after mild mechanical injury. Growth kinetics and cellular differentiation.", "content": "Mild mechanical abrasion of tracheal epithelium of Vitamin A deficient rats removed the superficial cells and spared basal cells which divided to repopulate the damaged area. The proliferative cells passed through a period of DNA synthesis with the greatest numbers of thymidine incorporating cells in samples labelled 22 h after injury. A peak of cell division occurred at 32 h and there was no further DNA synthesis or cell division. The area of wounding exhibited squamous metaplasia while normal pseudostratified muco-ciliary structure was retained by adjacent epithelium which had not been injured. The data indicates that squamous metaplasia in the respiratory epithelium in longstanding Vitamin A deficiency is due to redirected differentiation of basal cells and is seen only after mitotic activity has occurred.", "contents": "Regeneration of vitamin A deficient rat tracheal epithelium after mild mechanical injury. Growth kinetics and cellular differentiation. Mild mechanical abrasion of tracheal epithelium of Vitamin A deficient rats removed the superficial cells and spared basal cells which divided to repopulate the damaged area. The proliferative cells passed through a period of DNA synthesis with the greatest numbers of thymidine incorporating cells in samples labelled 22 h after injury. A peak of cell division occurred at 32 h and there was no further DNA synthesis or cell division. The area of wounding exhibited squamous metaplasia while normal pseudostratified muco-ciliary structure was retained by adjacent epithelium which had not been injured. The data indicates that squamous metaplasia in the respiratory epithelium in longstanding Vitamin A deficiency is due to redirected differentiation of basal cells and is seen only after mitotic activity has occurred."}
{"id": "PMID:478214", "title": "Quantal division and a postmitotic state in myoblast differentiation.", "content": "The reversible arrest of myoblast differentiation by ethidium bromide (EB) has been used to examine the nature of the transition from the proliferative state to terminal differentiation resulting in fusion into muscle fibers. If EB is introduced at the time that myoblasts are shifted to medium that induces fusion, all apparent cytodifferentiation is suspended. When such EB arrested myoblasts are released from EB inhibition they fuse without reentering the cell cycle. If EB arrested myoblasts are released into proliferation promoting medium rather than medium that induces fusion they neither fuse nor proliferate. In this case they remain quiescent in the proliferating medium for an extended period, however, if these myoblasts are subsequently shifted to medium that induces fusion, they fuse without reentering the cell cycle. Apparently the myoblasts have become postmitotic and competent to fuse into muscle fibers during their initial exposure to fusion inducing medium, even though cytodifferentiation has been blocked. Exposure of these postmitotic fusion competent myoblasts to proliferation promoting medium does not stimulate them to reenter the cell cycle but does prevent fusion into muscle fibers. These results are most consistent with a quantal division model of myoblast differentiation rather than a gradual transition from the proliferative state to a state in which fusion occurs.", "contents": "Quantal division and a postmitotic state in myoblast differentiation. The reversible arrest of myoblast differentiation by ethidium bromide (EB) has been used to examine the nature of the transition from the proliferative state to terminal differentiation resulting in fusion into muscle fibers. If EB is introduced at the time that myoblasts are shifted to medium that induces fusion, all apparent cytodifferentiation is suspended. When such EB arrested myoblasts are released from EB inhibition they fuse without reentering the cell cycle. If EB arrested myoblasts are released into proliferation promoting medium rather than medium that induces fusion they neither fuse nor proliferate. In this case they remain quiescent in the proliferating medium for an extended period, however, if these myoblasts are subsequently shifted to medium that induces fusion, they fuse without reentering the cell cycle. Apparently the myoblasts have become postmitotic and competent to fuse into muscle fibers during their initial exposure to fusion inducing medium, even though cytodifferentiation has been blocked. Exposure of these postmitotic fusion competent myoblasts to proliferation promoting medium does not stimulate them to reenter the cell cycle but does prevent fusion into muscle fibers. These results are most consistent with a quantal division model of myoblast differentiation rather than a gradual transition from the proliferative state to a state in which fusion occurs."}
{"id": "PMID:478217", "title": "[Precursors of arteriosclerosis in childhood. Protocol and firsts results concerning plasma lipids and carbohydrate tolerance in 520 school children at Pordenone (Italy) (author's transl)].", "content": "The cardiological centers of Pordenone and Cittadella (Italy) organized by the Institute of Clinica Medica II of Padua University, have carried out a study on the \"Precursors of arteriosclerosis in children\", according to a WHO protocol. In this paper some results of the Pordenone study are reported, concerning serum cholesterol (TC), triglycerides (TG) and blood glucose 1 h after 1 g/Kg glucose per os. 520 school children, males and females, aged 6, 9, 12, 15 years, entered the study. Mean serum TC resulted significantly higher at age 12 as compared to the other age classes. Serum TG progressively increased with age. Mean serum TC and TG in our italian children and adolescents were silimar to those reported in studies from other countries. Blood glucose resulted significantly higher in children than in adolescents. The distribution of the blood glucose values was bimodal. After having arbitrarily fixed cut-off points for serum TC, TG and blood glucose, the prevalence of hyperlipidemia and hyperglycemia was determined. The prevalence figures have shown that at the pediatric age it is possible to identify subjects at \"risk\" of developing arteriosclerosis.", "contents": "[Precursors of arteriosclerosis in childhood. Protocol and firsts results concerning plasma lipids and carbohydrate tolerance in 520 school children at Pordenone (Italy) (author's transl)]. The cardiological centers of Pordenone and Cittadella (Italy) organized by the Institute of Clinica Medica II of Padua University, have carried out a study on the \"Precursors of arteriosclerosis in children\", according to a WHO protocol. In this paper some results of the Pordenone study are reported, concerning serum cholesterol (TC), triglycerides (TG) and blood glucose 1 h after 1 g/Kg glucose per os. 520 school children, males and females, aged 6, 9, 12, 15 years, entered the study. Mean serum TC resulted significantly higher at age 12 as compared to the other age classes. Serum TG progressively increased with age. Mean serum TC and TG in our italian children and adolescents were silimar to those reported in studies from other countries. Blood glucose resulted significantly higher in children than in adolescents. The distribution of the blood glucose values was bimodal. After having arbitrarily fixed cut-off points for serum TC, TG and blood glucose, the prevalence of hyperlipidemia and hyperglycemia was determined. The prevalence figures have shown that at the pediatric age it is possible to identify subjects at \"risk\" of developing arteriosclerosis."}
{"id": "PMID:478218", "title": "[Transposition of the great arteries: anatomic types of left ventricular outflow tract obstruction (author's transl)].", "content": "A postmortem investigation has been carried out in 48 heart specimens with transposition of the great arteries (TGA) to evaluate incidence and type of left ventricular outflow tract obstruction. The frequence of pulmonary stenosis in our series (23%) is similar to those reported from previous studies. Cases have been divided in two groups according to presence or absence of ventricular septal defect. In cases with intact ventricular septum the obstruction was determined by asymmetric hypertrophy of the septum (1 observation) and by abnormal attachement of clefted anterior mitral leaflet to the interventricular septum (2 observations). In the specimens with ventricular septal defect, 4 presented a stenosis due to malalignment of the infundibular septum, associated with valvular stenosis in 1 case and with valvular stenosis and fibrous subvalvular ring in 2. Another case presented a stenotic pulmonary valve and a parachute mitral valve. The remaining 3 showed a cleft of the anterior leaflet of the mitral valve. The high incidence of infundibular malalignment obstruction is underlined together with its association with A-P or L-position of the aorta. This peculiar relation of the great arteries could be angiocardiographically employed as indicative for the presence of this type of left outflow tract obstruction in TGA.", "contents": "[Transposition of the great arteries: anatomic types of left ventricular outflow tract obstruction (author's transl)]. A postmortem investigation has been carried out in 48 heart specimens with transposition of the great arteries (TGA) to evaluate incidence and type of left ventricular outflow tract obstruction. The frequence of pulmonary stenosis in our series (23%) is similar to those reported from previous studies. Cases have been divided in two groups according to presence or absence of ventricular septal defect. In cases with intact ventricular septum the obstruction was determined by asymmetric hypertrophy of the septum (1 observation) and by abnormal attachement of clefted anterior mitral leaflet to the interventricular septum (2 observations). In the specimens with ventricular septal defect, 4 presented a stenosis due to malalignment of the infundibular septum, associated with valvular stenosis in 1 case and with valvular stenosis and fibrous subvalvular ring in 2. Another case presented a stenotic pulmonary valve and a parachute mitral valve. The remaining 3 showed a cleft of the anterior leaflet of the mitral valve. The high incidence of infundibular malalignment obstruction is underlined together with its association with A-P or L-position of the aorta. This peculiar relation of the great arteries could be angiocardiographically employed as indicative for the presence of this type of left outflow tract obstruction in TGA."}
{"id": "PMID:478219", "title": "[Clinical, hemodynamic and cineangiocardiolographic findings in congestive cardiomyopathy].", "content": "Sixteen patients affected by congestive cardiomyopathy were studied by means of right and left cardiac catheterization and cineangiocardiography. Cardiac output and mean pulmonary circulation time were determined by the radiocardiographic method. Left ventriculograms were obtained in all the patients in the 45 degrees RAO projection; the left ventricular end diastolic and end systolic volumes were calculated both by the area-length and by the slice method. Among the several hemodynamic data (stroke index, mean pulmonary circulation time, left ventricular end diastolic pressure, left ventricular volumes and ejection fraction) the most early impaired and therefore usefull for an early diagnosis, were: the left ventricular end-systolic volume, the left ventricular end-diastolic volume and the left ventricular ejection fraction.", "contents": "[Clinical, hemodynamic and cineangiocardiolographic findings in congestive cardiomyopathy]. Sixteen patients affected by congestive cardiomyopathy were studied by means of right and left cardiac catheterization and cineangiocardiography. Cardiac output and mean pulmonary circulation time were determined by the radiocardiographic method. Left ventriculograms were obtained in all the patients in the 45 degrees RAO projection; the left ventricular end diastolic and end systolic volumes were calculated both by the area-length and by the slice method. Among the several hemodynamic data (stroke index, mean pulmonary circulation time, left ventricular end diastolic pressure, left ventricular volumes and ejection fraction) the most early impaired and therefore usefull for an early diagnosis, were: the left ventricular end-systolic volume, the left ventricular end-diastolic volume and the left ventricular ejection fraction."}
{"id": "PMID:478221", "title": "[The effects of intravenous mexiletine on cardiac performance (author's transl)].", "content": "The effects of intravenous Mexiletine (K\u00f61173) on cardiac performance has been studied in 20 patients affected by myocardiosclerosis (10 patients were classified in the 1st or 2nd NYHA classes, 10 cases in the 3rd or 4th ones. Mexiletine (1.5 mg/Kg) was given intravenously over a period of 1 minute. The effect on myocardial function has been evaluated by means of the systolic time intervals (STI). The drug caused non-significant changes of the left ventricular ejection time (LVET); on the contrary it caused a significant increases of the pre-ejection period (PEP), isometric contraction time (ICT) and the PEP/LVET ratio. These alteration became evident after 5--10 minutes and disappeared about 30 minutes after administration of the drug.", "contents": "[The effects of intravenous mexiletine on cardiac performance (author's transl)]. The effects of intravenous Mexiletine (K\u00f61173) on cardiac performance has been studied in 20 patients affected by myocardiosclerosis (10 patients were classified in the 1st or 2nd NYHA classes, 10 cases in the 3rd or 4th ones. Mexiletine (1.5 mg/Kg) was given intravenously over a period of 1 minute. The effect on myocardial function has been evaluated by means of the systolic time intervals (STI). The drug caused non-significant changes of the left ventricular ejection time (LVET); on the contrary it caused a significant increases of the pre-ejection period (PEP), isometric contraction time (ICT) and the PEP/LVET ratio. These alteration became evident after 5--10 minutes and disappeared about 30 minutes after administration of the drug."}
{"id": "PMID:478225", "title": "[Supraventricular tachyarrhythmias. Therapeutical efficiency of bunaftine administed in rapid bolus (author's transl)].", "content": "The authors exhibit the results obtained in 25 patients sumministring bunaftine in 3 mg/Kg in 1-2 min in rapid bolus i.v. By means a continuous ECG registration they could state that the antiarrhythmic effect is simultaneous with the distribution of the drug, obtaining the restoration of the sinus rhythm in about 90% of the patients. The Authors remark the lack of meaningful collateral effect by means a pre-treatment with diazepam-cortisone, according to their method.", "contents": "[Supraventricular tachyarrhythmias. Therapeutical efficiency of bunaftine administed in rapid bolus (author's transl)]. The authors exhibit the results obtained in 25 patients sumministring bunaftine in 3 mg/Kg in 1-2 min in rapid bolus i.v. By means a continuous ECG registration they could state that the antiarrhythmic effect is simultaneous with the distribution of the drug, obtaining the restoration of the sinus rhythm in about 90% of the patients. The Authors remark the lack of meaningful collateral effect by means a pre-treatment with diazepam-cortisone, according to their method."}
{"id": "PMID:478246", "title": "[Electronmicroscopic studies on the skin pigmentation of the red bellied toad Bombina bombina (L.)].", "content": "The various colours of the red-bellied toad Bombina bombina are caused by 3 types of chromatophores, the red xanthophores, the white iridophores and the black melanophores. In the brownish skin and in the black spots of the dorsal region all types of chromatophores are found. But in the epidermis of the black spots there are more grana of melanin than in the other region of the back. The 3 types of chromatophores are not present in the skin of all parts of the body. The dark blue colour of the belly is caused only by melanophores, whereas in the ventral parts with light blue colour there occur melanophores and iridophores. The white spots of the belly contain only iridophores, the red spots have iridophores and xanthophores.", "contents": "[Electronmicroscopic studies on the skin pigmentation of the red bellied toad Bombina bombina (L.)]. The various colours of the red-bellied toad Bombina bombina are caused by 3 types of chromatophores, the red xanthophores, the white iridophores and the black melanophores. In the brownish skin and in the black spots of the dorsal region all types of chromatophores are found. But in the epidermis of the black spots there are more grana of melanin than in the other region of the back. The 3 types of chromatophores are not present in the skin of all parts of the body. The dark blue colour of the belly is caused only by melanophores, whereas in the ventral parts with light blue colour there occur melanophores and iridophores. The white spots of the belly contain only iridophores, the red spots have iridophores and xanthophores."}
{"id": "PMID:478248", "title": "[Maturing of fibrils and intraosseous osteogenesis as a basis for the development of lamellae and the osteon structure].", "content": "We investigated in continuation of lightmicroscopical (phasecontrast, polarized light) and statistical studies concerning the formation of lamellae and osteons electromicroscopical the nature of fibrils in different areas of the cross section of the metacarpus from a cattle fetus of 520 mm SSL. During the maturation of fibrils in a subperiostal trabecal the diameter of fibrils increased (465 to 470 A). The period (316 or 536 A) contains primarily only a broad band and a small interband. Subsequently the interband increased (221 or 215 A), but the band decreased (324 or 271 A). In the centre of trabeculae the fibrils formate microlamellae. The fibrils split later on in the medial part of the cross section in microfibrils with irregular oreintation and appear lightmicroscopical as intermediate tissue (blasse Mittellinie). At the same time new fibrils are synthesized around the osteocytes and their canaliculi. As the result of a kind of remodeling the fibrils arrange in a parallel order forming packages which appear lightmicroscopical as fibrous felts. These fibrous felts exhibits partly an periodical arrangement with a small band (164 A) and a wide interband (287 A). In a following stage in the fibrous felts lamellae are developed, the length of which increased around the medullary cavity. Firstly after the development of lamellae the definite bone fibrils are reconstituted (diameter: 530 A; band: 348 A; interband: 212 A). Apparently the osteocytes are responsible for these procedures in a stage specific synthetic and lytic activity. The modification of the nature of fibrils results probably after the dissolving of the structural glycoproteins, which stabilize the tropocollagen molecules. A reconstitution of the structural glycoproteins after formation of the lamellae induce the reconstitution of bone specific collagenfibrils. The regulating mechanism for the codification of these structural development are unknown.", "contents": "[Maturing of fibrils and intraosseous osteogenesis as a basis for the development of lamellae and the osteon structure]. We investigated in continuation of lightmicroscopical (phasecontrast, polarized light) and statistical studies concerning the formation of lamellae and osteons electromicroscopical the nature of fibrils in different areas of the cross section of the metacarpus from a cattle fetus of 520 mm SSL. During the maturation of fibrils in a subperiostal trabecal the diameter of fibrils increased (465 to 470 A). The period (316 or 536 A) contains primarily only a broad band and a small interband. Subsequently the interband increased (221 or 215 A), but the band decreased (324 or 271 A). In the centre of trabeculae the fibrils formate microlamellae. The fibrils split later on in the medial part of the cross section in microfibrils with irregular oreintation and appear lightmicroscopical as intermediate tissue (blasse Mittellinie). At the same time new fibrils are synthesized around the osteocytes and their canaliculi. As the result of a kind of remodeling the fibrils arrange in a parallel order forming packages which appear lightmicroscopical as fibrous felts. These fibrous felts exhibits partly an periodical arrangement with a small band (164 A) and a wide interband (287 A). In a following stage in the fibrous felts lamellae are developed, the length of which increased around the medullary cavity. Firstly after the development of lamellae the definite bone fibrils are reconstituted (diameter: 530 A; band: 348 A; interband: 212 A). Apparently the osteocytes are responsible for these procedures in a stage specific synthetic and lytic activity. The modification of the nature of fibrils results probably after the dissolving of the structural glycoproteins, which stabilize the tropocollagen molecules. A reconstitution of the structural glycoproteins after formation of the lamellae induce the reconstitution of bone specific collagenfibrils. The regulating mechanism for the codification of these structural development are unknown."}
{"id": "PMID:478249", "title": "[Histometric volume determination in aging human thyroid glands and possible errors of this method].", "content": "We made histometric volume analyses of hematoxylin and eosin stained paraffin sections of 43 human thyroid lobes by means of the electric integrating apparatus Eltinor 4 A (Optische Werke Rathenow). We investigated ageing changes of the relative amounts of epithel, colloid and connective tissue in the total volume. The volume density of colloid rises up to the 3rd decade, thereafter it shows a small decrease. On the contrary, the amount of epithelial cells decreases until the 3rd decade. The data concerning connective tissue exhibit an increasing trend up to senility. During such measurements errors appear, which are called, among others, total error, systematic and statistic error. We estimated these errors and demonstrated possibilities of their diminution.", "contents": "[Histometric volume determination in aging human thyroid glands and possible errors of this method]. We made histometric volume analyses of hematoxylin and eosin stained paraffin sections of 43 human thyroid lobes by means of the electric integrating apparatus Eltinor 4 A (Optische Werke Rathenow). We investigated ageing changes of the relative amounts of epithel, colloid and connective tissue in the total volume. The volume density of colloid rises up to the 3rd decade, thereafter it shows a small decrease. On the contrary, the amount of epithelial cells decreases until the 3rd decade. The data concerning connective tissue exhibit an increasing trend up to senility. During such measurements errors appear, which are called, among others, total error, systematic and statistic error. We estimated these errors and demonstrated possibilities of their diminution."}
{"id": "PMID:478250", "title": "[The evolution of the relations between brain development, secondary temporomandibular joint and facial development in mammals].", "content": "Comparing the development of the face in mammals and sauropsida only in mammals the maxillary process reach forward to fuse with the medial nasal fold. This is the supposition for the maxillary and mandibular process to fuse forming the cheeks. The phylogenetically relations for these developmental events are discussed.", "contents": "[The evolution of the relations between brain development, secondary temporomandibular joint and facial development in mammals]. Comparing the development of the face in mammals and sauropsida only in mammals the maxillary process reach forward to fuse with the medial nasal fold. This is the supposition for the maxillary and mandibular process to fuse forming the cheeks. The phylogenetically relations for these developmental events are discussed."}
{"id": "PMID:478251", "title": "[The problem of sugar resorption in the small intestine: histochemical studies by means of the Gomori method].", "content": "The role of alkaline phosphatase during active absorption of sugars is experimented with Gomori's histochemical method. Experiments on empty intestine show that the surgical intervention does not alter the phosphate content of the wall of the small intestine. Absorption experiments with 10% and 5,4% (isotonic) glucose solutions demonstrate a marked decrease in the alkaline phosphatase content of the wall of the small intestine. In half of the cases only, absorption experiments with a 10% mannitol solution determine a small decrease in the alkaline phosphatase of the wall of the small intestine. In 90% of the cases, there are lesions of the intestinal epithelium.", "contents": "[The problem of sugar resorption in the small intestine: histochemical studies by means of the Gomori method]. The role of alkaline phosphatase during active absorption of sugars is experimented with Gomori's histochemical method. Experiments on empty intestine show that the surgical intervention does not alter the phosphate content of the wall of the small intestine. Absorption experiments with 10% and 5,4% (isotonic) glucose solutions demonstrate a marked decrease in the alkaline phosphatase content of the wall of the small intestine. In half of the cases only, absorption experiments with a 10% mannitol solution determine a small decrease in the alkaline phosphatase of the wall of the small intestine. In 90% of the cases, there are lesions of the intestinal epithelium."}
{"id": "PMID:478252", "title": "[Comments and the diagnosis of vitality of early pregnancy in threatening abortion].", "content": "In a series of 79 consecutive patients examined by sonar and assays of human chorionic gonadotrophin in the first half of pregnancy 41 ultimately aborted. On analysis of the sonar and post abortion findings it was found, that the aborted pregnancies fell into 3 clearly defined groups: blighted ova, missed abortions and hydatidiform moles. Strict diagnostic sonar criteria of abnormality were established for these groups. Assays of human chorionic gonadotrophin have shown to be useful guides. But the overlap between their normal and abnormal ranges does not permit their use as sole arbitors of which pregnancies should be terminated.", "contents": "[Comments and the diagnosis of vitality of early pregnancy in threatening abortion]. In a series of 79 consecutive patients examined by sonar and assays of human chorionic gonadotrophin in the first half of pregnancy 41 ultimately aborted. On analysis of the sonar and post abortion findings it was found, that the aborted pregnancies fell into 3 clearly defined groups: blighted ova, missed abortions and hydatidiform moles. Strict diagnostic sonar criteria of abnormality were established for these groups. Assays of human chorionic gonadotrophin have shown to be useful guides. But the overlap between their normal and abnormal ranges does not permit their use as sole arbitors of which pregnancies should be terminated."}
{"id": "PMID:478253", "title": "[Prognostic and therapeutic implications of the antenatal care of multiple pregnancies].", "content": "From January 1967 to April 1978, 175 twin deliveries occurred among 19,935 deliveries (.88%). A comparison to the literature a low perinatal mortality of 4.57% (adjusted 2.84%) was noted. The reason for the 3 to 5 fold increase in perinatal mortality compared to single on pregnancies was investigated. Review of the records showed conditions specific for twin pregnancies. The treatment necessary to improve the prognosis for twin pregnancies was discussed. It is suggested that the perinatal mortality of 2% for twin pregnancies can be achieved with intensive antenatal care for twin pregnancies.", "contents": "[Prognostic and therapeutic implications of the antenatal care of multiple pregnancies]. From January 1967 to April 1978, 175 twin deliveries occurred among 19,935 deliveries (.88%). A comparison to the literature a low perinatal mortality of 4.57% (adjusted 2.84%) was noted. The reason for the 3 to 5 fold increase in perinatal mortality compared to single on pregnancies was investigated. Review of the records showed conditions specific for twin pregnancies. The treatment necessary to improve the prognosis for twin pregnancies was discussed. It is suggested that the perinatal mortality of 2% for twin pregnancies can be achieved with intensive antenatal care for twin pregnancies."}
{"id": "PMID:478256", "title": "[The ramining cervical stump (author's transl)].", "content": "The subtotal hysterectomy is no longer indicated for the treatment of female genital disease with rare exceptions. It has been known for a long time that the remaining cervical stump has very few advantages but many potential risks. Among our patients who had cervical stumps removed 32 of 69 cases had malignant changes in the cervical stump. Decensus and prolapse was the next common abnormality. If adnexal tumors or cervical cancers do not indicate an abdominal approach for the removal, the vaginal approach for the removal of the cervical stump ist preferred since this is easier and less risky.", "contents": "[The ramining cervical stump (author's transl)]. The subtotal hysterectomy is no longer indicated for the treatment of female genital disease with rare exceptions. It has been known for a long time that the remaining cervical stump has very few advantages but many potential risks. Among our patients who had cervical stumps removed 32 of 69 cases had malignant changes in the cervical stump. Decensus and prolapse was the next common abnormality. If adnexal tumors or cervical cancers do not indicate an abdominal approach for the removal, the vaginal approach for the removal of the cervical stump ist preferred since this is easier and less risky."}
{"id": "PMID:478257", "title": "[New considerations in therapeutic abortions using a second generation prostaglandins (author's transl)].", "content": "The results of 98 therapeutic abortions in the first and second trimester of pregnancy using F prostaglandins are reported. The prostaglandins were eigher administered by fractionated extra-aminotic PGF2 alpha, intramuscular 15-methyl PGF2 alpha or extra-amniotic or intracervical single shot 15-methyl PGF2 alpha. The induction abortion time of 13 hours and 15minutes for the single shot and 14 hours and 28 minutes for the intramuscular administratic with the prostaglandin derivatives is shorter than with natural prostaglandins which showed a mean induction to abortion time of 19 hours and 30 minutes. The blood loss was low in all groups at approximately 75 mls. Side effects are least with the single shot method (11%). Two patients had nausea, two patients had vomiting, three patients had flush or lower abdominal cramps. The intramuscular administration showed the highest frequency of side effects (80%). In the first trimester the single shot method always leads to sufficient cervical dilatation although the abortion rate was low at 59%. During the second trimester a high abortion rate is found. Therefore this method can be recommended for therapeutic abortions.", "contents": "[New considerations in therapeutic abortions using a second generation prostaglandins (author's transl)]. The results of 98 therapeutic abortions in the first and second trimester of pregnancy using F prostaglandins are reported. The prostaglandins were eigher administered by fractionated extra-aminotic PGF2 alpha, intramuscular 15-methyl PGF2 alpha or extra-amniotic or intracervical single shot 15-methyl PGF2 alpha. The induction abortion time of 13 hours and 15minutes for the single shot and 14 hours and 28 minutes for the intramuscular administratic with the prostaglandin derivatives is shorter than with natural prostaglandins which showed a mean induction to abortion time of 19 hours and 30 minutes. The blood loss was low in all groups at approximately 75 mls. Side effects are least with the single shot method (11%). Two patients had nausea, two patients had vomiting, three patients had flush or lower abdominal cramps. The intramuscular administration showed the highest frequency of side effects (80%). In the first trimester the single shot method always leads to sufficient cervical dilatation although the abortion rate was low at 59%. During the second trimester a high abortion rate is found. Therefore this method can be recommended for therapeutic abortions."}
{"id": "PMID:478258", "title": "[Present day status in therapy of toxemia of pregnancy (author's transl)].", "content": "Since the causes of toxemia of pregnancy are unknown, therapy is still symptomatic and is now determined by the most recent knowledge of the pathophysiology of the disease. Rest, a balanced, predominantly protein-rich diet and avoidance of stress are recommended as prophylactic treatment of toxemia of pregnancy in patients with a predisposition of the condition. Early recognition of the symptoms of toxemia of pregnancy is of great importance. Treatment of mild cases consists of bed rest, possibly supplemented by sedatives and a preponderantly proteinrich diet. Administration of diuretics is obsolete and sodium restriction is no longer recommended. Antihypertensives are seldom indicated. Overweight women are no longer maintained on specially low calorie diets. Severe cases of toxemia of pregnancy must be trated as inpatients under intensive care. Principles of treatment are: 1. Prevention of seizures (by sedation). 2. Improvement of the general condition of the women (especially circulation and renal function). 3. Delivery at an opportune time for mother and child. Treatment of eclampsia follows largely the same principles. In these cases, immediate delivery is required regardless of the condition of the fetus.", "contents": "[Present day status in therapy of toxemia of pregnancy (author's transl)]. Since the causes of toxemia of pregnancy are unknown, therapy is still symptomatic and is now determined by the most recent knowledge of the pathophysiology of the disease. Rest, a balanced, predominantly protein-rich diet and avoidance of stress are recommended as prophylactic treatment of toxemia of pregnancy in patients with a predisposition of the condition. Early recognition of the symptoms of toxemia of pregnancy is of great importance. Treatment of mild cases consists of bed rest, possibly supplemented by sedatives and a preponderantly proteinrich diet. Administration of diuretics is obsolete and sodium restriction is no longer recommended. Antihypertensives are seldom indicated. Overweight women are no longer maintained on specially low calorie diets. Severe cases of toxemia of pregnancy must be trated as inpatients under intensive care. Principles of treatment are: 1. Prevention of seizures (by sedation). 2. Improvement of the general condition of the women (especially circulation and renal function). 3. Delivery at an opportune time for mother and child. Treatment of eclampsia follows largely the same principles. In these cases, immediate delivery is required regardless of the condition of the fetus."}
{"id": "PMID:478259", "title": "[Recurrent carcinoma in situ of the uterine cervix (author's transl)].", "content": "Among 228 patients who had previous treatment for carcinoma in situ of the cervix, most by hysterectomy, 3 recurrences in vaginal stumps were detected in women age 36, 46, and 56. In another case a carcinoma in situ of the cervix was found in a 25 year old patient who had a diagnostic and therapeutic conization because of desire for childbearing. The histology of the cone showed no residual carcinoma. Concommitent to the recurrent carcinoma in situ of the cervix a carcinoma of the vulva was detected. Recurrences of carcinoma in situ could occur because of incomplete resection of primary lesion. Wrong classification in the laboratory is possible if the invasion is missed on the microscopic examination and the case therefore receives the wrong treatment. Recurrences which are not true recurrences could arise from multicentric atypical foci of the squamous epitheleum of the -ower female genital tract. These considerations lead to the following recommendations: 1. When the suspected diagnosis carcinoma in situ of the uterine cervix is made a large enough cone biopsy which is appropriately marked is obtained and a complete microscopic examination of the cone must be obtained. 2. A hysterectomy with a large vaginal cuff is the treatment of choice. 3. A careful workup of the operative specimen in the microscopic laboratory is expecially essential. 4. A patient with such previous treatment for carcinoma in situ of the uterine cervix must be urged to continue with regular preventive examinations for cancer. The vaginal stump must be regularly re-examined both by colposcopy and by cytology.", "contents": "[Recurrent carcinoma in situ of the uterine cervix (author's transl)]. Among 228 patients who had previous treatment for carcinoma in situ of the cervix, most by hysterectomy, 3 recurrences in vaginal stumps were detected in women age 36, 46, and 56. In another case a carcinoma in situ of the cervix was found in a 25 year old patient who had a diagnostic and therapeutic conization because of desire for childbearing. The histology of the cone showed no residual carcinoma. Concommitent to the recurrent carcinoma in situ of the cervix a carcinoma of the vulva was detected. Recurrences of carcinoma in situ could occur because of incomplete resection of primary lesion. Wrong classification in the laboratory is possible if the invasion is missed on the microscopic examination and the case therefore receives the wrong treatment. Recurrences which are not true recurrences could arise from multicentric atypical foci of the squamous epitheleum of the -ower female genital tract. These considerations lead to the following recommendations: 1. When the suspected diagnosis carcinoma in situ of the uterine cervix is made a large enough cone biopsy which is appropriately marked is obtained and a complete microscopic examination of the cone must be obtained. 2. A hysterectomy with a large vaginal cuff is the treatment of choice. 3. A careful workup of the operative specimen in the microscopic laboratory is expecially essential. 4. A patient with such previous treatment for carcinoma in situ of the uterine cervix must be urged to continue with regular preventive examinations for cancer. The vaginal stump must be regularly re-examined both by colposcopy and by cytology."}
{"id": "PMID:478260", "title": "[Religion and marriage (author's transl)].", "content": "Marriage according to religious denomination is no longer important because of the philosophical changes in recent times. Reproductive behaviour is no longer dependant on religion for the same reasons. Faith in the naturalness and ordainment by God of the roles of the sexes is no longer prevalent. From 1960--1976 the number of marriages in the Federal Republic of Germany decreased by 30%. The number of divorces increased by 122%. Mixed interdenominational marriages remained steady at between 20--25% of all marriages. Marriages of partners of the same denomination decreased from 76.5 to 68.6%. The marriages oe changed by man and individualized this trend is positive. Intervention of the states leads to conditions which should be avoided, namely that the interhuman relationships become insecure a fact which can be easily abused by demagogues.", "contents": "[Religion and marriage (author's transl)]. Marriage according to religious denomination is no longer important because of the philosophical changes in recent times. Reproductive behaviour is no longer dependant on religion for the same reasons. Faith in the naturalness and ordainment by God of the roles of the sexes is no longer prevalent. From 1960--1976 the number of marriages in the Federal Republic of Germany decreased by 30%. The number of divorces increased by 122%. Mixed interdenominational marriages remained steady at between 20--25% of all marriages. Marriages of partners of the same denomination decreased from 76.5 to 68.6%. The marriages oe changed by man and individualized this trend is positive. Intervention of the states leads to conditions which should be avoided, namely that the interhuman relationships become insecure a fact which can be easily abused by demagogues."}
{"id": "PMID:478261", "title": "[The behaviour of serum-coeruplasmin in women with intrauterine pessary containing copper (author's transl)].", "content": "In 50 women before and 10--35 weeks after insertion of a Copper-T-200 serum concentration of Caeruloplasmin was assayed. A decrease of serum concentration with statistical significance (p less than 0,0001) was determined. In 22 women before and 4--5 weeks after removal of a Copper-T-200 serum concentration of Caeruloplasmin was assayed. An increase of serum concentration with statistical significance (p less than 0.01) was determined.", "contents": "[The behaviour of serum-coeruplasmin in women with intrauterine pessary containing copper (author's transl)]. In 50 women before and 10--35 weeks after insertion of a Copper-T-200 serum concentration of Caeruloplasmin was assayed. A decrease of serum concentration with statistical significance (p less than 0,0001) was determined. In 22 women before and 4--5 weeks after removal of a Copper-T-200 serum concentration of Caeruloplasmin was assayed. An increase of serum concentration with statistical significance (p less than 0.01) was determined."}
{"id": "PMID:478262", "title": "[Long-term outcome of sterilization as a function of the indication, marital status, age, number of children and concurrently performed therapeutic abortions (author's transl)].", "content": "On the basis of questionnaires 1084 sterilized women were interrogated concerning their satisfaction with sterilization. 769 (70.9%) fully completed questionnaires could be evaluated. 96.4% of women were satisfied with their sterilization. 1.3% of patients requested reversal of sterilization. Non satisfactory results occured mainly in women where the indication for sterilization was on non-social basis, in single women and in cases where an interruption of pregnancy was performed at the same time. No correlation was found between age or parity and the satisfactory outcome of sterilization.", "contents": "[Long-term outcome of sterilization as a function of the indication, marital status, age, number of children and concurrently performed therapeutic abortions (author's transl)]. On the basis of questionnaires 1084 sterilized women were interrogated concerning their satisfaction with sterilization. 769 (70.9%) fully completed questionnaires could be evaluated. 96.4% of women were satisfied with their sterilization. 1.3% of patients requested reversal of sterilization. Non satisfactory results occured mainly in women where the indication for sterilization was on non-social basis, in single women and in cases where an interruption of pregnancy was performed at the same time. No correlation was found between age or parity and the satisfactory outcome of sterilization."}
{"id": "PMID:478263", "title": "[Determination of the plasma ribonuclease activity as a diagnostic aid in the diagnosis of ovarian cancer. Results of a double blind study (author's transl)].", "content": "The determination of the plasmaribonucleases was reported by Sheid and co-workers in 1977 for the diagnosis of carcinoma of the ovary. The authors investigated the accuracy of this test with a modified method in a double blind study encompassing healthy controls. Blood samples from 62 patients with ovarian cancer were tested. The results were accurate in 56 cases. In 6 cases the test results were doubtful. The difference in the ribonuclease activity was significant between patients with ovarian cancer and healthy patients or patients in remission from ovarian cancer. The results were not dependant upon the FIGO staging of the disease. Our results encourage the recommendation to use this test as a diagnostic aid in carcinoma of the ovary.", "contents": "[Determination of the plasma ribonuclease activity as a diagnostic aid in the diagnosis of ovarian cancer. Results of a double blind study (author's transl)]. The determination of the plasmaribonucleases was reported by Sheid and co-workers in 1977 for the diagnosis of carcinoma of the ovary. The authors investigated the accuracy of this test with a modified method in a double blind study encompassing healthy controls. Blood samples from 62 patients with ovarian cancer were tested. The results were accurate in 56 cases. In 6 cases the test results were doubtful. The difference in the ribonuclease activity was significant between patients with ovarian cancer and healthy patients or patients in remission from ovarian cancer. The results were not dependant upon the FIGO staging of the disease. Our results encourage the recommendation to use this test as a diagnostic aid in carcinoma of the ovary."}
{"id": "PMID:478265", "title": "[Determination of low blood glucose levels in newborns with the reflectance photometer (author's transl)].", "content": "The paper reports the examination of a new test strip Reflotest-Hypoglycemie, which was developed specifically for the hypoglycemic range. In 2 series of 110 respectively 120 samples of capillary blood taken from premature and full term infants the blood glucose concentrations were determined using Reflotest-Hypoglycemie and the hexokineasured with the test strip agree well with the results of hexokinase method in a range less than 50 mg/dl. Bilirubin and hematocrit values do not affect the glucose test. Glutathion and ascorbic acid interfere with the test strip method but this error is neglectable because of physiological low concentrations of these substances.", "contents": "[Determination of low blood glucose levels in newborns with the reflectance photometer (author's transl)]. The paper reports the examination of a new test strip Reflotest-Hypoglycemie, which was developed specifically for the hypoglycemic range. In 2 series of 110 respectively 120 samples of capillary blood taken from premature and full term infants the blood glucose concentrations were determined using Reflotest-Hypoglycemie and the hexokineasured with the test strip agree well with the results of hexokinase method in a range less than 50 mg/dl. Bilirubin and hematocrit values do not affect the glucose test. Glutathion and ascorbic acid interfere with the test strip method but this error is neglectable because of physiological low concentrations of these substances."}
{"id": "PMID:478266", "title": "[History and course of pregnancy in a group of cerclage patients. A comparative study (I) (author's transl)].", "content": "The previous history and course of pregnancy in 357 women with variable births, in whom cerclage had been performed during 1966-1976 at the Freiburg University Gynaecological Hospital, were compared with a group of 380 women without cerclage, the age, parity and period during which birth had occurred, being identical. The high average of the patients with cerclage (29.3 years), the low percentage of primigravidae (17.1%), the threefold increased rate of abortions and early births (28.4%) as well as the high perinatal mortality (21.5%) during the preceding pregnancies, confirmed these women as a \"risk\" group. After cerclage, births before the 38th week were reduced from 41.1% to 24.6% (p less than 0.001.) 52.3% of the premature births occurred between the 36th and completed 37th week.  Perinatal mortality dropped to 5.6% (p less than 0.00l.). A clinically relevant gestosis was less frequent (0.8%;p less than 0.00l.). The fact that the rate of premature births in the cerclage group (p less than 0.001) continued to be elevated, is mainly attributed to the frequency of rupture of the amnion before the 38th week (21.0%).", "contents": "[History and course of pregnancy in a group of cerclage patients. A comparative study (I) (author's transl)]. The previous history and course of pregnancy in 357 women with variable births, in whom cerclage had been performed during 1966-1976 at the Freiburg University Gynaecological Hospital, were compared with a group of 380 women without cerclage, the age, parity and period during which birth had occurred, being identical. The high average of the patients with cerclage (29.3 years), the low percentage of primigravidae (17.1%), the threefold increased rate of abortions and early births (28.4%) as well as the high perinatal mortality (21.5%) during the preceding pregnancies, confirmed these women as a \"risk\" group. After cerclage, births before the 38th week were reduced from 41.1% to 24.6% (p less than 0.001.) 52.3% of the premature births occurred between the 36th and completed 37th week.  Perinatal mortality dropped to 5.6% (p less than 0.00l.). A clinically relevant gestosis was less frequent (0.8%;p less than 0.00l.). The fact that the rate of premature births in the cerclage group (p less than 0.001) continued to be elevated, is mainly attributed to the frequency of rupture of the amnion before the 38th week (21.0%)."}
{"id": "PMID:478267", "title": "[Ultrasonic biometry of the fetal crown-rump length between 7 and 20 weeks gestation (author's transl)].", "content": "In 370 examinations on 258 patients with known gestational age crown-rump length (CRL) of the fetus was determined by sonar between 7 and 20 weeks. From the results the mean and \"2 SD\" limits of CRL were derived by means of a non linear regression analysis. The measurements were further analysed to determine gestational age for a given CRL. Besides a velocity curve of CRL growth was calculated. It showed a maximum of 1.8 mm per day at 14 weeks. It was confirmated that sonar measurements of crown-rump length are very useful to estimate maturity of the fetus. The accuracy was found to be best in early measurements (+/-6 days at 7 weeks -+/-11 days at 20 weeks). In regard to clinical decisions reading errors have to be taken into consideration. To avoid them control examinations should be carried out.", "contents": "[Ultrasonic biometry of the fetal crown-rump length between 7 and 20 weeks gestation (author's transl)]. In 370 examinations on 258 patients with known gestational age crown-rump length (CRL) of the fetus was determined by sonar between 7 and 20 weeks. From the results the mean and \"2 SD\" limits of CRL were derived by means of a non linear regression analysis. The measurements were further analysed to determine gestational age for a given CRL. Besides a velocity curve of CRL growth was calculated. It showed a maximum of 1.8 mm per day at 14 weeks. It was confirmated that sonar measurements of crown-rump length are very useful to estimate maturity of the fetus. The accuracy was found to be best in early measurements (+/-6 days at 7 weeks -+/-11 days at 20 weeks). In regard to clinical decisions reading errors have to be taken into consideration. To avoid them control examinations should be carried out."}
{"id": "PMID:478268", "title": "[A new therapeutic approach for terminating intact and disturbed pregnancies: three years of experience with the prostaglandin E2-derivative sulprostone (SHB 286) (author's transl)].", "content": "Sulprostone, a new synthetic prostaglandin E2-derivate has been tested for artificial abortion in 628 women with intact or disturbed pregnancies in all stages of gestation. Compared with other prostaglandins used for this purpose systemic side-effects were low and success-rate was high. Systemic routes of application (constant intravenous drop infusion, intramuscular injection) were evaluted to be more successful and conventient than the local ones (extra- and intraamniotic). Suppositories showed no clinical success. Based on our expereince recommendations for the treatment with sulprostone are given: Intramuscular injections for inducing early abortion or menstrual regulation (less than 6th week of pregnancy) without necessity of curettage and for cervical rpiening (less than 12th week of pregnancy) prior to curettage, constant intravenous drop infusion for induction of abortion in second and third trimester pregnancy.", "contents": "[A new therapeutic approach for terminating intact and disturbed pregnancies: three years of experience with the prostaglandin E2-derivative sulprostone (SHB 286) (author's transl)]. Sulprostone, a new synthetic prostaglandin E2-derivate has been tested for artificial abortion in 628 women with intact or disturbed pregnancies in all stages of gestation. Compared with other prostaglandins used for this purpose systemic side-effects were low and success-rate was high. Systemic routes of application (constant intravenous drop infusion, intramuscular injection) were evaluted to be more successful and conventient than the local ones (extra- and intraamniotic). Suppositories showed no clinical success. Based on our expereince recommendations for the treatment with sulprostone are given: Intramuscular injections for inducing early abortion or menstrual regulation (less than 6th week of pregnancy) without necessity of curettage and for cervical rpiening (less than 12th week of pregnancy) prior to curettage, constant intravenous drop infusion for induction of abortion in second and third trimester pregnancy."}
{"id": "PMID:478269", "title": "[Investigation of the epidemilogy of fungal vaginitis in 1000 patients (author's transl)].", "content": "In the out-patient department and antenatal clinic 1000 patients were examined for yeast of the genital tract by the nickerson-medium. In 10.3% of the patients the cultures showed yeast. The wet-mount preparation examination under phase contrast microscopy was compared to the nickerson-medi-m culture. Patients with a past history of yeast infections or patients with an intrauterine device showed a statistically significant increase in the rate of yeast vaginitis compared to the control groups. Patients who had antibiotics or were treated with corticosteroids showed a higher incidence of yeast vaginitis than normal controls. Controls had a 9% incidence. The number of cases was too small for statistical analysis. The incidence of yeast vaginitis in patients on combined or sequential oral contraceptives was the same as in the control groups. The pregnant patients and obese patients did not show an increased incidence of yeast. High risk patients for yeast vaginitis are defined in which a culture for yeast is jusitfied. Especially antenatal patients in the last trimestre are a high risk group because of the possibility of neonatal thrush.", "contents": "[Investigation of the epidemilogy of fungal vaginitis in 1000 patients (author's transl)]. In the out-patient department and antenatal clinic 1000 patients were examined for yeast of the genital tract by the nickerson-medium. In 10.3% of the patients the cultures showed yeast. The wet-mount preparation examination under phase contrast microscopy was compared to the nickerson-medi-m culture. Patients with a past history of yeast infections or patients with an intrauterine device showed a statistically significant increase in the rate of yeast vaginitis compared to the control groups. Patients who had antibiotics or were treated with corticosteroids showed a higher incidence of yeast vaginitis than normal controls. Controls had a 9% incidence. The number of cases was too small for statistical analysis. The incidence of yeast vaginitis in patients on combined or sequential oral contraceptives was the same as in the control groups. The pregnant patients and obese patients did not show an increased incidence of yeast. High risk patients for yeast vaginitis are defined in which a culture for yeast is jusitfied. Especially antenatal patients in the last trimestre are a high risk group because of the possibility of neonatal thrush."}
{"id": "PMID:478270", "title": "[HCG-determinations in serum and mammary cyst aspirates in women with benign breast tumors (author's transl)].", "content": "Results of HCG-determinations in human serum and cystfluid of benign mammary tumors are presented. HCG was determined gy RIA in 40 samples from 30 patients with different forms of fibro-cystic mastitis. In 60% of the cases positive results were obtained. It is still unclear, whether the demonstrated substance is native HCG, a free beta-subunit, a mixture of both or a different substance with immunologic properties similar to those of HCG.", "contents": "[HCG-determinations in serum and mammary cyst aspirates in women with benign breast tumors (author's transl)]. Results of HCG-determinations in human serum and cystfluid of benign mammary tumors are presented. HCG was determined gy RIA in 40 samples from 30 patients with different forms of fibro-cystic mastitis. In 60% of the cases positive results were obtained. It is still unclear, whether the demonstrated substance is native HCG, a free beta-subunit, a mixture of both or a different substance with immunologic properties similar to those of HCG."}
{"id": "PMID:478271", "title": "[Fetal death in utero 15 weeks following amniocentesis -- a legal case (author's transl)].", "content": "This is a report about fetal death in utero in a case of monozygote, monochrionic, monoamnionic twin pregnancy (46,xy) 15 weeks after amniocentesis. The public prosecutor was occupied with the case by the patient. The accusation run as follows: Stab wound with following death in utero of one twin and thereby damage of the second twin also with following death in utero. Directly after delivery the first diagnosis by aspect of the fetuses was: transfusion syndrome. The following endoradiography of the blood vessels of the umbilical chords and placenta confirmed the first diagnosis (anastomosis). Additionally both chords were entangled and knotted variously. It was concluded that the extremely smaller fetus died from malnutrition (transfusion syndrome) whereas the taller fetus died from acute asphyxia (knotted chords in monoamnionic twin pregnancy). The forensic investigation was, as aspected, without any pathological findings concerning sequelae of stab wounds but revealed the lack of one chordvessel of the smaller fetus and the typical histological findings of an aspiration lung of the taller fetus.", "contents": "[Fetal death in utero 15 weeks following amniocentesis -- a legal case (author's transl)]. This is a report about fetal death in utero in a case of monozygote, monochrionic, monoamnionic twin pregnancy (46,xy) 15 weeks after amniocentesis. The public prosecutor was occupied with the case by the patient. The accusation run as follows: Stab wound with following death in utero of one twin and thereby damage of the second twin also with following death in utero. Directly after delivery the first diagnosis by aspect of the fetuses was: transfusion syndrome. The following endoradiography of the blood vessels of the umbilical chords and placenta confirmed the first diagnosis (anastomosis). Additionally both chords were entangled and knotted variously. It was concluded that the extremely smaller fetus died from malnutrition (transfusion syndrome) whereas the taller fetus died from acute asphyxia (knotted chords in monoamnionic twin pregnancy). The forensic investigation was, as aspected, without any pathological findings concerning sequelae of stab wounds but revealed the lack of one chordvessel of the smaller fetus and the typical histological findings of an aspiration lung of the taller fetus."}
{"id": "PMID:478272", "title": "[Induction of labour in cases of intrauterine fetal death by cervical dilatation with a balloon and oxytocin titration (author's transl)].", "content": "In twelve cases of intrauterine fetal death labour was induced by combined treatment with an intra-cervical balloon and oxytocin infusion. The average induction-delivery time was approximately 11 hours. Acceptance of this method for induction of labour by the patients was satisfactory and the side effects were minor.", "contents": "[Induction of labour in cases of intrauterine fetal death by cervical dilatation with a balloon and oxytocin titration (author's transl)]. In twelve cases of intrauterine fetal death labour was induced by combined treatment with an intra-cervical balloon and oxytocin infusion. The average induction-delivery time was approximately 11 hours. Acceptance of this method for induction of labour by the patients was satisfactory and the side effects were minor."}
{"id": "PMID:478273", "title": "[Radioimmunoassay of beta sub unit HCG following hydatidiform mole or chorionic carcinoma (author's transl)].", "content": "The radioimmunoassay of Beta Sub Unit HCG in the serum heralds a marked improvement in the diagnosis of gestational trophoblastic disease. In nine cases of hydatidiform mole and three cases of chorionic carcinoma serial examinations of the Beta Sub Unit HCG were performed. When the immunologic pregnancy test became negative following a hydatidiform mole or following treatment of chorionic carcinoma, the HCG excretion remained above detectable values for several more weeks. The decrease of Beta Sub Unit serum HCG was either linear or in fluctuations. The detection of fluctuations or a secondary rise in the Beta Sub Unit HCG titre permits the early diagnosis of invasive trophoblastic disease following hydatidiform mole or of insufficient treatment or recurrence in cases of chorionic carcinoma. Our results suggest that the optimal follow-up with Beta Sub Unit serum HCG is by weekly determinations until four consecutive determinations remained negative. This is followed by two determinations at bi-weekly intervals and thereafter monthly follow-up examinations.", "contents": "[Radioimmunoassay of beta sub unit HCG following hydatidiform mole or chorionic carcinoma (author's transl)]. The radioimmunoassay of Beta Sub Unit HCG in the serum heralds a marked improvement in the diagnosis of gestational trophoblastic disease. In nine cases of hydatidiform mole and three cases of chorionic carcinoma serial examinations of the Beta Sub Unit HCG were performed. When the immunologic pregnancy test became negative following a hydatidiform mole or following treatment of chorionic carcinoma, the HCG excretion remained above detectable values for several more weeks. The decrease of Beta Sub Unit serum HCG was either linear or in fluctuations. The detection of fluctuations or a secondary rise in the Beta Sub Unit HCG titre permits the early diagnosis of invasive trophoblastic disease following hydatidiform mole or of insufficient treatment or recurrence in cases of chorionic carcinoma. Our results suggest that the optimal follow-up with Beta Sub Unit serum HCG is by weekly determinations until four consecutive determinations remained negative. This is followed by two determinations at bi-weekly intervals and thereafter monthly follow-up examinations."}
{"id": "PMID:478274", "title": "[The population law (author's transl)].", "content": "Population Law is not the same at all times and in all places. Each economic stage of development has its own population law. The different development of the means of production changes the conditions. Future conditions of economy are going to be significantly different from those in the past. First the reduction of the number in population, second the changing age structure of the population, third the saturation with many material goods, fourth the decrease of the domestic growth of the gross national product and distribution, fifth in creasing environmental problems with resistance to tolerate polluting industries, sixth changes in the world economic division of preoduction with an increased production of manufactured products from developing countries, and seventh growth in the population lead to a sifting effect which pushes the weaker groups of the population into a subproletareate.", "contents": "[The population law (author's transl)]. Population Law is not the same at all times and in all places. Each economic stage of development has its own population law. The different development of the means of production changes the conditions. Future conditions of economy are going to be significantly different from those in the past. First the reduction of the number in population, second the changing age structure of the population, third the saturation with many material goods, fourth the decrease of the domestic growth of the gross national product and distribution, fifth in creasing environmental problems with resistance to tolerate polluting industries, sixth changes in the world economic division of preoduction with an increased production of manufactured products from developing countries, and seventh growth in the population lead to a sifting effect which pushes the weaker groups of the population into a subproletareate."}
{"id": "PMID:478285", "title": "Aggression during competition: effects of age, sex, and amount and type of provocation.", "content": "Three experiments were designed to examine the expression of instrumental and hostile aggression by 6- to 10-year-old children during a competitive game. The rate at which a child pressed a button which allegedly interfered with his opponent's progress on the task was the measure of instrumental aggression. Pressing a second button presumably caused one's opponent to hear an aversive noise and served as the measure of hostile aggression. The task was designed in an attempt to isolate (a) frustration from attack as the instigator of aggression and (b) instrumental from hostile aggression as the desired outcome. In all three experiments the measures of instrumental and hostile aggression were generally highly correlated except under provocation conditions during which the number of attacks exceeded the number of frustrations. This suggested that the task was successful in tapping two different classes of aggressive responding. In Experiment 1 older children (N = 161 males and females) expressed more of both instrumental and hostile aggression than did younger children, and males were more aggressive than females on both measures. However, neither measure of aggression varied as a function of level of provocation. This finding was discrepant from the authors' previous research which had focused solely on instrumental aggression. Apparently, the introduction of attack and the opportunity for hostile responding changed the experimental situation. In order to focus more directly on this issue, Experiments 2 (N = 60 males) and 3 (N = 56 males) used various combinations of attack and frustration with 9- and 10-year-old males. Attack, prior to frustration, elicited the greatest amount of hostile aggression. Frustration, with no attack, resulted in the highest level of instrumental aggression. The complex relationship between these sources of provocation and instrumental and hostile aggression was discussed.", "contents": "Aggression during competition: effects of age, sex, and amount and type of provocation. Three experiments were designed to examine the expression of instrumental and hostile aggression by 6- to 10-year-old children during a competitive game. The rate at which a child pressed a button which allegedly interfered with his opponent's progress on the task was the measure of instrumental aggression. Pressing a second button presumably caused one's opponent to hear an aversive noise and served as the measure of hostile aggression. The task was designed in an attempt to isolate (a) frustration from attack as the instigator of aggression and (b) instrumental from hostile aggression as the desired outcome. In all three experiments the measures of instrumental and hostile aggression were generally highly correlated except under provocation conditions during which the number of attacks exceeded the number of frustrations. This suggested that the task was successful in tapping two different classes of aggressive responding. In Experiment 1 older children (N = 161 males and females) expressed more of both instrumental and hostile aggression than did younger children, and males were more aggressive than females on both measures. However, neither measure of aggression varied as a function of level of provocation. This finding was discrepant from the authors' previous research which had focused solely on instrumental aggression. Apparently, the introduction of attack and the opportunity for hostile responding changed the experimental situation. In order to focus more directly on this issue, Experiments 2 (N = 60 males) and 3 (N = 56 males) used various combinations of attack and frustration with 9- and 10-year-old males. Attack, prior to frustration, elicited the greatest amount of hostile aggression. Frustration, with no attack, resulted in the highest level of instrumental aggression. The complex relationship between these sources of provocation and instrumental and hostile aggression was discussed."}
{"id": "PMID:478286", "title": "[Cytogenetic effects induced in vitro in human peripheral blood lymphocytes by neutrons. II. Relative biological effectiveness of neutrons having different energies].", "content": "Human lymphocytes were irradiated in vitro during Go stage by graded doses of thermal neutrons and neutrons having an average energy of 0.04; 0.09; 0.35; 0.85 and 14,7 MeV as well as by 60Co gamma rays, and RBE of neutrons relative to gamma-rays was calculated for the frequency of total and different types of aberrations. It was found that the RBE has the most value at the low doses and decreases when the exposition dose increases. 0.35 MeV neutrons have the maximum RBE in comparison with neutrons having other energies. When comparing the RBE values calculated for different types of chromosome aberrations, it was found out that dicentrics and dicentrics plus centric rings had more RBE than acentric aberrations (pair fragments and minutes).", "contents": "[Cytogenetic effects induced in vitro in human peripheral blood lymphocytes by neutrons. II. Relative biological effectiveness of neutrons having different energies]. Human lymphocytes were irradiated in vitro during Go stage by graded doses of thermal neutrons and neutrons having an average energy of 0.04; 0.09; 0.35; 0.85 and 14,7 MeV as well as by 60Co gamma rays, and RBE of neutrons relative to gamma-rays was calculated for the frequency of total and different types of aberrations. It was found that the RBE has the most value at the low doses and decreases when the exposition dose increases. 0.35 MeV neutrons have the maximum RBE in comparison with neutrons having other energies. When comparing the RBE values calculated for different types of chromosome aberrations, it was found out that dicentrics and dicentrics plus centric rings had more RBE than acentric aberrations (pair fragments and minutes)."}
{"id": "PMID:478287", "title": "[Quantitative analysis of sister chromatid exchanges in the cell].", "content": "Assuming a random nature of distribution of sister chromatid exchanges (SCE) in a karyotype, the formulae have been obtained allowing the calculation of the number of SCE that are overlooked because of a limited resolving power of the SCE detection method. The results obtained mean that the actual number of SCE is more than the observed one, the part of overlooked exchanges being increased with the heightening of the SCE level. Taking into account overlook exchanges, the formula has been obtained that makes possible the calculation of the expected number of SCE observed in any group of chromosomes. These results were applied in the analysis of the SCE distribution among chromosomes. A better conformity has been obtained between the expected results and the observed ones, than under the assumption that the observed SCE are distributed in proportion to the lengths of chromosomes. The obtained formulae are of use in interpreting the lack of the observed SCE in small chromosomes and the excess of them in large ones.", "contents": "[Quantitative analysis of sister chromatid exchanges in the cell]. Assuming a random nature of distribution of sister chromatid exchanges (SCE) in a karyotype, the formulae have been obtained allowing the calculation of the number of SCE that are overlooked because of a limited resolving power of the SCE detection method. The results obtained mean that the actual number of SCE is more than the observed one, the part of overlooked exchanges being increased with the heightening of the SCE level. Taking into account overlook exchanges, the formula has been obtained that makes possible the calculation of the expected number of SCE observed in any group of chromosomes. These results were applied in the analysis of the SCE distribution among chromosomes. A better conformity has been obtained between the expected results and the observed ones, than under the assumption that the observed SCE are distributed in proportion to the lengths of chromosomes. The obtained formulae are of use in interpreting the lack of the observed SCE in small chromosomes and the excess of them in large ones."}
{"id": "PMID:478288", "title": "[Correlation of heredity and environmental factors in the etiology of Vilyui encephalomyelitis. I. Patient frequency in families].", "content": "Viljuisk encephalomyelitis (VE) is a severe neurologic disease characterized by slow progressive dementia', oligobradykinesia, low spastic paraparesis and speech disturbances. It develops in persons of 20-50 years old. VE occurs in a small region of middle Viljui, but for last years the focus has considerably expanded. Etiology of VE is still obscure. 194 families with VE patients were examined. The data obtained contradict the hypothesis of simple recessive inheritance of VE. The value of the heredity coefficient, calculated on the basis of the Falconer - Edwards model, is 22-29% for relatives of the first relation degree. It suggests the existence of individual hereditary determined susceptibility to VE. 14 secondary cases were observed in affected families among adopted relatives (adopted children, husbands and wives of patients) with a rate exceeding random possible frequency. These observations have been evaluated as an evidence of horizontal transmission of the disease from patients with chronic forms to healthy persons. Obligatory condition for the transmission consists in a long-term contact (as a rule, more than one year). According to all known characteristics VE should be refferred to slow infections.", "contents": "[Correlation of heredity and environmental factors in the etiology of Vilyui encephalomyelitis. I. Patient frequency in families]. Viljuisk encephalomyelitis (VE) is a severe neurologic disease characterized by slow progressive dementia', oligobradykinesia, low spastic paraparesis and speech disturbances. It develops in persons of 20-50 years old. VE occurs in a small region of middle Viljui, but for last years the focus has considerably expanded. Etiology of VE is still obscure. 194 families with VE patients were examined. The data obtained contradict the hypothesis of simple recessive inheritance of VE. The value of the heredity coefficient, calculated on the basis of the Falconer - Edwards model, is 22-29% for relatives of the first relation degree. It suggests the existence of individual hereditary determined susceptibility to VE. 14 secondary cases were observed in affected families among adopted relatives (adopted children, husbands and wives of patients) with a rate exceeding random possible frequency. These observations have been evaluated as an evidence of horizontal transmission of the disease from patients with chronic forms to healthy persons. Obligatory condition for the transmission consists in a long-term contact (as a rule, more than one year). According to all known characteristics VE should be refferred to slow infections."}
{"id": "PMID:478297", "title": "Nucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome.", "content": "Using the DNA sequence method of Maxam and Gilbert the entire nucleotide sequence of the adenovirus 2 EcoRI-F fragment was determined. Information contained in that nucleotide sequence, which is 1743 base pairs long, is interpreted with respect to the mapping and processing of the three mRNAs partly encoded by the EcoRI-F fragment. A method to rapidly determine the cleavage site of restriction endonucleases is also reported.", "contents": "Nucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome. Using the DNA sequence method of Maxam and Gilbert the entire nucleotide sequence of the adenovirus 2 EcoRI-F fragment was determined. Information contained in that nucleotide sequence, which is 1743 base pairs long, is interpreted with respect to the mapping and processing of the three mRNAs partly encoded by the EcoRI-F fragment. A method to rapidly determine the cleavage site of restriction endonucleases is also reported."}
{"id": "PMID:478298", "title": "A new site-specific endonuclease showing phenotypical crypticity in a tumorigenic strain of Agrobacterium tumefaciens.", "content": "AtuBVI, an endonuclease showing new site-specificity, has been isolated from the tumorigenic strain IIBV7 of Agrobacterium tumefaciens, and is undetectable in the non-tumorigenic sister strain IIBNV6. AtuBVI degrades IIBV7 DNA in vitro and should, therefore, be regarded as being phenotypically cryptic in the bacterial cell; it also shows anomalous behavior under cerain incubation conditions. These properties point to a possible role for this enzyme in the insertion of exogenous Ti-plasmid DNA into plant tissues during tumorigenesis.", "contents": "A new site-specific endonuclease showing phenotypical crypticity in a tumorigenic strain of Agrobacterium tumefaciens. AtuBVI, an endonuclease showing new site-specificity, has been isolated from the tumorigenic strain IIBV7 of Agrobacterium tumefaciens, and is undetectable in the non-tumorigenic sister strain IIBNV6. AtuBVI degrades IIBV7 DNA in vitro and should, therefore, be regarded as being phenotypically cryptic in the bacterial cell; it also shows anomalous behavior under cerain incubation conditions. These properties point to a possible role for this enzyme in the insertion of exogenous Ti-plasmid DNA into plant tissues during tumorigenesis."}
{"id": "PMID:478299", "title": "The nucleotide sequence of the transforming HindIII-G fragment of adenovirus type 5 DNA. The region between map positions 4.5 (HpaI site) and 8.0 (HindIII site).", "content": "The nucleotide sequence of the region between map positions 4.5 (HpaI-site) and 8.0 (HindIII-site) of adenovirus type 5 (Ad5) DNA has been determined. This stretch of DNA is part of the transforming HindIII-G fragment, which is 2809 nucleotides long. The sequenced segment was found to have a long open reading frame for protein biosynthesis, starting 23 nucleotides from the HpaI site and extending all the way to the HindIII-G site, which could code for a protein of at least 44 000 daltons. The possible correlation beteen the coding capacity of the HindIII-G fragment and the \"transforming\" proteins specified by it will be discussed in the light of the recent data on the splicing of early mRNAs.", "contents": "The nucleotide sequence of the transforming HindIII-G fragment of adenovirus type 5 DNA. The region between map positions 4.5 (HpaI site) and 8.0 (HindIII site). The nucleotide sequence of the region between map positions 4.5 (HpaI-site) and 8.0 (HindIII-site) of adenovirus type 5 (Ad5) DNA has been determined. This stretch of DNA is part of the transforming HindIII-G fragment, which is 2809 nucleotides long. The sequenced segment was found to have a long open reading frame for protein biosynthesis, starting 23 nucleotides from the HpaI site and extending all the way to the HindIII-G site, which could code for a protein of at least 44 000 daltons. The possible correlation beteen the coding capacity of the HindIII-G fragment and the \"transforming\" proteins specified by it will be discussed in the light of the recent data on the splicing of early mRNAs."}
{"id": "PMID:478300", "title": "Electrophoretic mobility of high-molecular-weight, double-stranded DNA on agarose gels.", "content": "A new theoretical model for the migration of high-molecular-weight, double-stranded DNA on agarose gels is presented. This leads to the prediction that under certain conditions of electrophoresis, a linear relationship will exist between the molecular weight of a DNA molecule, raised to the (-2/3) power, and its electrophoretic mobility. Agarose gel electrophoresis of the fragments of bacteriophage lambda DNA produced by several restriction endonucleases confirms this relationship, and establishes some of the limits on its linearity. For this work, a polyacrylamide slab gel apparatus was modified for use with agarose gels. This apparatus has several advantages over others commercially available for agarose gel electrophoresis, including the abilities to run a larger number of samples at one time, to use lower-concentration gels, and to maintain better temperature stability across the width of the gel. The validation of the relationship developed here between molecular weight and electrophoretic mobility should make this a useful method for determining the molecular weights of DNA fragments.", "contents": "Electrophoretic mobility of high-molecular-weight, double-stranded DNA on agarose gels. A new theoretical model for the migration of high-molecular-weight, double-stranded DNA on agarose gels is presented. This leads to the prediction that under certain conditions of electrophoresis, a linear relationship will exist between the molecular weight of a DNA molecule, raised to the (-2/3) power, and its electrophoretic mobility. Agarose gel electrophoresis of the fragments of bacteriophage lambda DNA produced by several restriction endonucleases confirms this relationship, and establishes some of the limits on its linearity. For this work, a polyacrylamide slab gel apparatus was modified for use with agarose gels. This apparatus has several advantages over others commercially available for agarose gel electrophoresis, including the abilities to run a larger number of samples at one time, to use lower-concentration gels, and to maintain better temperature stability across the width of the gel. The validation of the relationship developed here between molecular weight and electrophoretic mobility should make this a useful method for determining the molecular weights of DNA fragments."}
{"id": "PMID:478301", "title": "Primary structure of an EcoRI fragment of lambda imm434 DNA containing regions cI-cro of phage 434 and cII-o of phage lambda.", "content": "Digestion of phage lambda imm434 DNA with restriction endonuclease EcoRI yields 7 fragments. The shortest among them (1287 bp) contains the right part of the phage 434 immunity region and the phage DNA portion proximal to it. The complete primary structure of this fragment has been determined using the chemical method of DNA sequencing. Hypothetical amino-acid sequences of proteins coded by the cro gene of phage 434 and the cII gene of phage lambda, as well as NH2-terminal amino-acid sequences of the cI protein of phage 434 and the O protein of phage lambda, have been deduced solely on the basis of the DNA sequence. The fragment studied contains also the pR and probably prm promoters and the oR operator of phage 434. The sequence coding for them differs from the respective DNA sequence of phage lambda.", "contents": "Primary structure of an EcoRI fragment of lambda imm434 DNA containing regions cI-cro of phage 434 and cII-o of phage lambda. Digestion of phage lambda imm434 DNA with restriction endonuclease EcoRI yields 7 fragments. The shortest among them (1287 bp) contains the right part of the phage 434 immunity region and the phage DNA portion proximal to it. The complete primary structure of this fragment has been determined using the chemical method of DNA sequencing. Hypothetical amino-acid sequences of proteins coded by the cro gene of phage 434 and the cII gene of phage lambda, as well as NH2-terminal amino-acid sequences of the cI protein of phage 434 and the O protein of phage lambda, have been deduced solely on the basis of the DNA sequence. The fragment studied contains also the pR and probably prm promoters and the oR operator of phage 434. The sequence coding for them differs from the respective DNA sequence of phage lambda."}
{"id": "PMID:478302", "title": "Physical mapping of the globin gene deletion in (delta beta (0)) -thalassaemia.", "content": "We have constructed a physical map of restriction endonuclease cleavage sites in the (delta (+) beta)-globin gene region in the DNA of patients with (delta beta(0))-thalassaemia. This map shows that a 10 kb deletion has occured in (delta beta (0))-thalassaemia to remove the entire beta-globin gene and the 3' portion of the delta-globin gene. The 5' terminus of the deletion is in the large intron of the delta-globin gene and the 3' terminus 1.8 kb to the 3'-side of the beta-globin gene. A similar deletion of about 7 kb has been described previously in the DNA of patients with Hb Lepore; the 5' terminus of the deletion is also in the delta-globin gene but the 3' terminus is in the beta-globin gene. Comparison of the foetal (gamma) globin gene expression in adults with (delta beta(0))-thalassaemia and Hba Lepore suggests that the 3' extragenic regions of the beta-globin gene contain DNA sequences involved in the regulation of gamma-globulin gene expression.", "contents": "Physical mapping of the globin gene deletion in (delta beta (0)) -thalassaemia. We have constructed a physical map of restriction endonuclease cleavage sites in the (delta (+) beta)-globin gene region in the DNA of patients with (delta beta(0))-thalassaemia. This map shows that a 10 kb deletion has occured in (delta beta (0))-thalassaemia to remove the entire beta-globin gene and the 3' portion of the delta-globin gene. The 5' terminus of the deletion is in the large intron of the delta-globin gene and the 3' terminus 1.8 kb to the 3'-side of the beta-globin gene. A similar deletion of about 7 kb has been described previously in the DNA of patients with Hb Lepore; the 5' terminus of the deletion is also in the delta-globin gene but the 3' terminus is in the beta-globin gene. Comparison of the foetal (gamma) globin gene expression in adults with (delta beta(0))-thalassaemia and Hba Lepore suggests that the 3' extragenic regions of the beta-globin gene contain DNA sequences involved in the regulation of gamma-globulin gene expression."}
{"id": "PMID:478313", "title": "Do you know the treatment of choice in peripheral arterial occlusive disease?", "content": "Five early warning signs can alert you to the presence of acute arterial occlusive disease, which can come on abruptly. It is crucial to differentiate between embolism and thrombosis, since treatment of the former is relatively simple, whereas the latter requires a more complicated surgical procedure.", "contents": "Do you know the treatment of choice in peripheral arterial occlusive disease? Five early warning signs can alert you to the presence of acute arterial occlusive disease, which can come on abruptly. It is crucial to differentiate between embolism and thrombosis, since treatment of the former is relatively simple, whereas the latter requires a more complicated surgical procedure."}
{"id": "PMID:478314", "title": "Treatment of depression in the elderly.", "content": "Depression is often overlooked, misdiagnosed, or inadequately treated in older patients. Since depressions secondary to drug use or organic diseases are common in the aged, complete physical and laboratory evaluation is essential. Fortunately, depression can be alleviated if you know the therapeutic options. Selection of the best one should be made carefully in elderly patients.", "contents": "Treatment of depression in the elderly. Depression is often overlooked, misdiagnosed, or inadequately treated in older patients. Since depressions secondary to drug use or organic diseases are common in the aged, complete physical and laboratory evaluation is essential. Fortunately, depression can be alleviated if you know the therapeutic options. Selection of the best one should be made carefully in elderly patients."}
{"id": "PMID:478315", "title": "Pitfalls in the early diagnosis of diabetes.", "content": "The glucose tolerance test remains the most acceptable approach to the early diagnosis of mild diabetes in adults in the absence of significantly elevated fasting blood sugar levels. Current reappraisal of what is a \"normal\" fasting blood glucose level is necessary to avoid the error of false-positive diagnosis of diabetes.", "contents": "Pitfalls in the early diagnosis of diabetes. The glucose tolerance test remains the most acceptable approach to the early diagnosis of mild diabetes in adults in the absence of significantly elevated fasting blood sugar levels. Current reappraisal of what is a \"normal\" fasting blood glucose level is necessary to avoid the error of false-positive diagnosis of diabetes."}
{"id": "PMID:478316", "title": "Emergency evaluation of head injuries in the elderly.", "content": "The first hour after head injury in the elderly is the most critical. Airway obstruction and impaired cardiovascular reflex mechanisms are the major initial problems. A patient with an extracerebral hematoma may need emergency trephination. You must look for the specific clinical indications or use a CAT scan to identify surgically accessible lesions.", "contents": "Emergency evaluation of head injuries in the elderly. The first hour after head injury in the elderly is the most critical. Airway obstruction and impaired cardiovascular reflex mechanisms are the major initial problems. A patient with an extracerebral hematoma may need emergency trephination. You must look for the specific clinical indications or use a CAT scan to identify surgically accessible lesions."}
{"id": "PMID:478317", "title": "Drug/alcohol interactions: avert potential dangers.", "content": "Alcoholism is not uncommon among older people, but \"binge\" or facilitative drinking is more characteristic of this age group. Alcohol interacts with many drugs frequently prescribed for older patients--antidepressants, sedatives, hypnotics, tranquilizers, and others--and the combination can sometimes be fatal.", "contents": "Drug/alcohol interactions: avert potential dangers. Alcoholism is not uncommon among older people, but \"binge\" or facilitative drinking is more characteristic of this age group. Alcohol interacts with many drugs frequently prescribed for older patients--antidepressants, sedatives, hypnotics, tranquilizers, and others--and the combination can sometimes be fatal."}
{"id": "PMID:478387", "title": "The effect of insulin on elder phenylketonuric patients.", "content": "The effect of insulin on blood phenylalanine, tyrosine and catecholamine levels was investigated in six phenylketonuric patients and eight normal controls. The plasma phenylalanine-tyrosine ratio was lower after insulin injection in five of six patients. In four patients the plasma catecholamine concentrations increased. The tyramine level was remarkably high one hour after insulin injection. Urinary homovanillic acid was elevated in four patients. It appeared that insulin treatment produced some therapeutic effects in patients with smaller phenylalanine-tyrosine ratios or elevated catecholamine levels.", "contents": "The effect of insulin on elder phenylketonuric patients. The effect of insulin on blood phenylalanine, tyrosine and catecholamine levels was investigated in six phenylketonuric patients and eight normal controls. The plasma phenylalanine-tyrosine ratio was lower after insulin injection in five of six patients. In four patients the plasma catecholamine concentrations increased. The tyramine level was remarkably high one hour after insulin injection. Urinary homovanillic acid was elevated in four patients. It appeared that insulin treatment produced some therapeutic effects in patients with smaller phenylalanine-tyrosine ratios or elevated catecholamine levels."}
{"id": "PMID:478388", "title": "Computed tomographic findings in subacute sclerosing panencephalitis.", "content": "We used a computed tomography to look for evidence of cerebral changes in a three-year-10-month-old girl with SSPE. The area of decreased density on CT corresponded to inflammatory and demyelinative lesions in the brain. CT scans seem to be a useful tool for more accurate assessment in the diagnosis of SSPE.", "contents": "Computed tomographic findings in subacute sclerosing panencephalitis. We used a computed tomography to look for evidence of cerebral changes in a three-year-10-month-old girl with SSPE. The area of decreased density on CT corresponded to inflammatory and demyelinative lesions in the brain. CT scans seem to be a useful tool for more accurate assessment in the diagnosis of SSPE."}
{"id": "PMID:478389", "title": "Mortality and causes of death in the aged with organic brain symptoms.", "content": "By various mental status rating methods, 363 aged residents at a nursing home (aged 80 years on the average) were previously evaluated with regard to chronic brain syndrome (CBS), and now at two and four years thereafter, the relation between mortality and the degree of CBS was statistically tested. The results showed that the severer the CBS, the higher the mortality, with significant difference. In 136 autopsy cases, the primary cause of death was investigated. The results showed no significant difference in the cause of death, either between dementia and non-dementia groups or between groups of mild, moderate and severe CBS.", "contents": "Mortality and causes of death in the aged with organic brain symptoms. By various mental status rating methods, 363 aged residents at a nursing home (aged 80 years on the average) were previously evaluated with regard to chronic brain syndrome (CBS), and now at two and four years thereafter, the relation between mortality and the degree of CBS was statistically tested. The results showed that the severer the CBS, the higher the mortality, with significant difference. In 136 autopsy cases, the primary cause of death was investigated. The results showed no significant difference in the cause of death, either between dementia and non-dementia groups or between groups of mild, moderate and severe CBS."}
{"id": "PMID:478390", "title": "An autopsy case of protracted necrotic encephalitis with marked atrophy of unilateral temporal lobe.", "content": "Clinical and neuropathological findings in a case of an unusually protracted encephalitis have been described. The patient was a 44-year-old man who experienced mental deterioration, right abducens and facial paresis, right and, subsequently, left hemiparesis, and consciousness disturbance with an intermittent low grade fever and occasional headache during one year and three months. Electroenphalograms showed periodic lateralized epileptiform discharges. Virus titers including herpes simplex virus were all normal. Neuropathological examinations revealed marked atrophic changes in the right temporal lobe, insular lobe and hippocampus with minimal inflammatory signs. The distribution of the lesions was almost identical with that of acute necrotic encephalitis or herpes simplex encephalitis despite its unusually protracted course.", "contents": "An autopsy case of protracted necrotic encephalitis with marked atrophy of unilateral temporal lobe. Clinical and neuropathological findings in a case of an unusually protracted encephalitis have been described. The patient was a 44-year-old man who experienced mental deterioration, right abducens and facial paresis, right and, subsequently, left hemiparesis, and consciousness disturbance with an intermittent low grade fever and occasional headache during one year and three months. Electroenphalograms showed periodic lateralized epileptiform discharges. Virus titers including herpes simplex virus were all normal. Neuropathological examinations revealed marked atrophic changes in the right temporal lobe, insular lobe and hippocampus with minimal inflammatory signs. The distribution of the lesions was almost identical with that of acute necrotic encephalitis or herpes simplex encephalitis despite its unusually protracted course."}
{"id": "PMID:478391", "title": "A 10-year follow-up study of 97 epileptics.", "content": "1) Of 267 patients with epilepsy who were examined in our clinic during the period between 1964 and 1966, 97 were investigated in a 10-year follow-up study. The patients were selected according to the following criteria: the age of onset of seizures was 10 years or older and the follow-up period was 10 years after the onset of seizures. Of the 97 patients, 36 were followed up by mail. 2) In the adult 10-year follow-up study, seizures disappeared in 43.3%, decreased in 34.0%, remained unchanged in 16.5%, and were aggravated in 6.2% of the 97 patients. Comparing the five-year follow-up study with the present study, the rate of cases which were free of seizures for three years was 22.7% in the five-year study and 43.3% in this study. The seizures were controlled for at least three years in 22.9% of the cases with partial seizure and in 63.3% of the cases with generalized seizure. The worst prognoses were found in cases with versive seizure in which the seizure was unchanged and/or aggravated in 4 (44.4%) of 9 cases. Of 16 cases with psychomotor seizure, 25% were unchanged or aggravated. 3) Comparing the impaired consciousness seizure with the psychomotor seizure, there was a tendency for the psychomotor seizures to remain as a single type of partial seizure, while the impaired consciousness seizure tended to develop easily into a secondarily generalized seizure. 4) Of the 97 patients, three were personality disorders in 19 cases (19.6%), episodic psychotic state in 2 cases (2.1%), and mental retardation in 6 cases (6.2%). Disorders of mood, irritability and explosiveness associated with personality disorders were improved in three of six cases whose seizures disappeared. Personality disorders were found in half of those cases with partial complex seizures. No correlation was found between mental symptoms and the prognosis of seizures. 5) The EEG was improved in 15 (27.8%) of a total of 54 cases, unchanged in 35 (64.8%) and aggravated in 4 (7.4%). In comparing the prognosis of clinical seizure with changes in the EEG, the seizures improved in 40 (74.1%) of the cases, whereas the EEG improved in only 15 (27.8%) of 54 cases. Most of the clinical seizures were controlled or decreased, whether the EEG improved or not.", "contents": "A 10-year follow-up study of 97 epileptics. 1) Of 267 patients with epilepsy who were examined in our clinic during the period between 1964 and 1966, 97 were investigated in a 10-year follow-up study. The patients were selected according to the following criteria: the age of onset of seizures was 10 years or older and the follow-up period was 10 years after the onset of seizures. Of the 97 patients, 36 were followed up by mail. 2) In the adult 10-year follow-up study, seizures disappeared in 43.3%, decreased in 34.0%, remained unchanged in 16.5%, and were aggravated in 6.2% of the 97 patients. Comparing the five-year follow-up study with the present study, the rate of cases which were free of seizures for three years was 22.7% in the five-year study and 43.3% in this study. The seizures were controlled for at least three years in 22.9% of the cases with partial seizure and in 63.3% of the cases with generalized seizure. The worst prognoses were found in cases with versive seizure in which the seizure was unchanged and/or aggravated in 4 (44.4%) of 9 cases. Of 16 cases with psychomotor seizure, 25% were unchanged or aggravated. 3) Comparing the impaired consciousness seizure with the psychomotor seizure, there was a tendency for the psychomotor seizures to remain as a single type of partial seizure, while the impaired consciousness seizure tended to develop easily into a secondarily generalized seizure. 4) Of the 97 patients, three were personality disorders in 19 cases (19.6%), episodic psychotic state in 2 cases (2.1%), and mental retardation in 6 cases (6.2%). Disorders of mood, irritability and explosiveness associated with personality disorders were improved in three of six cases whose seizures disappeared. Personality disorders were found in half of those cases with partial complex seizures. No correlation was found between mental symptoms and the prognosis of seizures. 5) The EEG was improved in 15 (27.8%) of a total of 54 cases, unchanged in 35 (64.8%) and aggravated in 4 (7.4%). In comparing the prognosis of clinical seizure with changes in the EEG, the seizures improved in 40 (74.1%) of the cases, whereas the EEG improved in only 15 (27.8%) of 54 cases. Most of the clinical seizures were controlled or decreased, whether the EEG improved or not."}
{"id": "PMID:478392", "title": "Electron-microscopical study on senile plaques in Alzheimer's disease.", "content": "The cerebral cortex taken post mortem from a case of Alzheimer's disease was examined especially with regard to the relation between blood vessels and senile plaques. Many senile plaques had central cores, composed of such matter as degenerated blood vessels and basement membranes with abundant amyloid fibrils. The components of senile plaques seemed to be degenerated neuronal and glial tissue compressed by developed basement membranes and amyloid fibrils. From this we would like to emphasize that senile plaques seem to be caused by amyloid fibrils and degenerated capillaries.", "contents": "Electron-microscopical study on senile plaques in Alzheimer's disease. The cerebral cortex taken post mortem from a case of Alzheimer's disease was examined especially with regard to the relation between blood vessels and senile plaques. Many senile plaques had central cores, composed of such matter as degenerated blood vessels and basement membranes with abundant amyloid fibrils. The components of senile plaques seemed to be degenerated neuronal and glial tissue compressed by developed basement membranes and amyloid fibrils. From this we would like to emphasize that senile plaques seem to be caused by amyloid fibrils and degenerated capillaries."}
{"id": "PMID:478393", "title": "Amyotrophic lateral sclerosis with temporal lobe atrophy.", "content": "Clinical and neuropathological findings are reported on a 48-year-old man who developed progressive bulbar palsy, muscle atrophy of four extremities, and hyperreflexia. Duration of the illness was about 19 months. Moderate personality changes were observed during his hospitalization. Neuropathological examination revealed the presence of an ALS: severe loss of the large motoneurons in the spinal anterior horns, and degeneration of the corticospinal tract, more prominent on the left side. The hypoglossal, the facial, and the motor trigeminal nuclei were also involved, but the oculomotor, the trochlear and the abducens nuclei were well preserved. There was bilateral, but more pronounced on the right, atrophy of the temporal poles consistent with the lesions of the temporal types of Pick's disease. This case indicates the simultaneous occurrence of degenerative diseases of the CNS, and the correspondence of laterality between the temporal lobe and the spinal cord may suggest a common etiology of these two types of disease processes.", "contents": "Amyotrophic lateral sclerosis with temporal lobe atrophy. Clinical and neuropathological findings are reported on a 48-year-old man who developed progressive bulbar palsy, muscle atrophy of four extremities, and hyperreflexia. Duration of the illness was about 19 months. Moderate personality changes were observed during his hospitalization. Neuropathological examination revealed the presence of an ALS: severe loss of the large motoneurons in the spinal anterior horns, and degeneration of the corticospinal tract, more prominent on the left side. The hypoglossal, the facial, and the motor trigeminal nuclei were also involved, but the oculomotor, the trochlear and the abducens nuclei were well preserved. There was bilateral, but more pronounced on the right, atrophy of the temporal poles consistent with the lesions of the temporal types of Pick's disease. This case indicates the simultaneous occurrence of degenerative diseases of the CNS, and the correspondence of laterality between the temporal lobe and the spinal cord may suggest a common etiology of these two types of disease processes."}
{"id": "PMID:478413", "title": "[The necessity of preventive gynecological studies in the aged].", "content": "According to epidemiological data the majority of the old women suffers from mammary cancer or gynecological malignoma. In cervical uterine cancers this ratio reaches more than 30% and in uterine body, ovarial and breast cancers more than 60%. Thus, it is significantly clear, that gynecological preventive medical examinations are so important. For cervical cancer cytology and for breast cancer mammography and thermography are valuable and satisfactory methods. Because of the lack of a practicable preventive examination method for corpus cancer and ovarial carcinoma it is evident, that regular clinical investigations are of vital importance in this field. Vulva and vaginal cancers are to be mentioned as real old women's neoplastic disease, because it starts mostly between 7th and 9th decade of life.", "contents": "[The necessity of preventive gynecological studies in the aged]. According to epidemiological data the majority of the old women suffers from mammary cancer or gynecological malignoma. In cervical uterine cancers this ratio reaches more than 30% and in uterine body, ovarial and breast cancers more than 60%. Thus, it is significantly clear, that gynecological preventive medical examinations are so important. For cervical cancer cytology and for breast cancer mammography and thermography are valuable and satisfactory methods. Because of the lack of a practicable preventive examination method for corpus cancer and ovarial carcinoma it is evident, that regular clinical investigations are of vital importance in this field. Vulva and vaginal cancers are to be mentioned as real old women's neoplastic disease, because it starts mostly between 7th and 9th decade of life."}
{"id": "PMID:478414", "title": "[Peritoneal lavage for the early diagnosis of intraabdominal injuries in blunt traumas. Personal results].", "content": "Peritoneal lavage has been found to be a simple, uncomplicated method of examination providing much information in cases of blunt abdominal trauma. It permits decision to start surgical procedures in adequate trauma at short notice and is performed routinely at our clinic in patients suffering from craniocerebral trauma, even in the absence of clinical symptoms of intraabdominal lesions. The advantage of the method is little need of time, personnel and surgery as well as the fact that it can be applied also by relatively unskilled doctors and does not really require a specialist. The precision of the method is high. An important factor for successful application is the use of sufficient quantities of a lavage solution.", "contents": "[Peritoneal lavage for the early diagnosis of intraabdominal injuries in blunt traumas. Personal results]. Peritoneal lavage has been found to be a simple, uncomplicated method of examination providing much information in cases of blunt abdominal trauma. It permits decision to start surgical procedures in adequate trauma at short notice and is performed routinely at our clinic in patients suffering from craniocerebral trauma, even in the absence of clinical symptoms of intraabdominal lesions. The advantage of the method is little need of time, personnel and surgery as well as the fact that it can be applied also by relatively unskilled doctors and does not really require a specialist. The precision of the method is high. An important factor for successful application is the use of sufficient quantities of a lavage solution."}
{"id": "PMID:478416", "title": "[New pathways in the treatment of psychologically drug dependent adolescents].", "content": "Based on a one-and-half year experience in a small group setting with drug dependent adolescents the change of group structure, the functioning of the group for its members, the leader's position within the group and the therapeutic relationship are analysed. In its beginning the group develops what can be called a \"symbiotic group structure\". Its transformation into that of a \"working group\" is the main task of therapy. The underlying processes can be compared with those of the beginning individuation in early childhood. Ego development begins, as M. Mahler and other modern investigators have shown, with the perception of one's own body and its representation in early ego formation. Using self awareness techniques, as we have done, is in this context a theoretical as well as practical well founded approach to the problems of psychic dependence.", "contents": "[New pathways in the treatment of psychologically drug dependent adolescents]. Based on a one-and-half year experience in a small group setting with drug dependent adolescents the change of group structure, the functioning of the group for its members, the leader's position within the group and the therapeutic relationship are analysed. In its beginning the group develops what can be called a \"symbiotic group structure\". Its transformation into that of a \"working group\" is the main task of therapy. The underlying processes can be compared with those of the beginning individuation in early childhood. Ego development begins, as M. Mahler and other modern investigators have shown, with the perception of one's own body and its representation in early ego formation. Using self awareness techniques, as we have done, is in this context a theoretical as well as practical well founded approach to the problems of psychic dependence."}
{"id": "PMID:478417", "title": "[Ureteral polyposis].", "content": "In connection with a case report a review is given on the clinical aspects of ureteral polyposis. The symptomatology of this condition differs considerably. The most frequent symptom is gross hematuria. Ureteral polyposis will be verified in most cases by a retrograde ureteropyelogram. Resection of the tumor bearing ureteral segment should be performed in order to exclude a malignant tumor.", "contents": "[Ureteral polyposis]. In connection with a case report a review is given on the clinical aspects of ureteral polyposis. The symptomatology of this condition differs considerably. The most frequent symptom is gross hematuria. Ureteral polyposis will be verified in most cases by a retrograde ureteropyelogram. Resection of the tumor bearing ureteral segment should be performed in order to exclude a malignant tumor."}
{"id": "PMID:478429", "title": "Chromatographic pattern of gut glucagon-like immunoreactivity (GLI) in plasma before and during glucose absorption.", "content": "In this work we have studied the chromatographic pattern on Bio-Gel P-30 columns of the glucagon-like immunoreactivity (GLI) present in unextracted plasma from normal dogs in the basal state and after intraduodenal administration of glucose. Basal plasma GLI, measured by R-8 antiserum, was distributed in four distinct fractions, whose approximate molecular weights were: greater than 30000 delta (Fraction I), 10000 delta (Fraction II), 3500 delta (Fraction III) and 2000 delta (Fraction IV). Fraction I accounted for the highest percent of total immunoreactivity. The increase in plasma GLI during glucose absorption was due to a significant increase of Fraction II, which may well correspond to tissue GLI Peak I, while no significant changes were evident in the other three fractions. The fact that tissue Peak I (or plasma Fraction II) ssems to be the factor secreted during glucose absorption puts the material/s of this molecular size in the first place for further investigation.", "contents": "Chromatographic pattern of gut glucagon-like immunoreactivity (GLI) in plasma before and during glucose absorption. In this work we have studied the chromatographic pattern on Bio-Gel P-30 columns of the glucagon-like immunoreactivity (GLI) present in unextracted plasma from normal dogs in the basal state and after intraduodenal administration of glucose. Basal plasma GLI, measured by R-8 antiserum, was distributed in four distinct fractions, whose approximate molecular weights were: greater than 30000 delta (Fraction I), 10000 delta (Fraction II), 3500 delta (Fraction III) and 2000 delta (Fraction IV). Fraction I accounted for the highest percent of total immunoreactivity. The increase in plasma GLI during glucose absorption was due to a significant increase of Fraction II, which may well correspond to tissue GLI Peak I, while no significant changes were evident in the other three fractions. The fact that tissue Peak I (or plasma Fraction II) ssems to be the factor secreted during glucose absorption puts the material/s of this molecular size in the first place for further investigation."}
{"id": "PMID:478430", "title": "Pancreatic exocrine secretion in the pig following test meals of different composition and intra-duodenal loads of glucose and maltose.", "content": "Eleven pigs were fitted with pancreatic and duodenal fistulae, and pancreatic juice collected permanently. Amylase, chymotrypsin, lipase and total proteins were determined in juice collected within 2 and 6 hours after different test-meals or intraduodenal loads of glucose and maltose. In the pancreatic juice of pigs adapted to a high-lipid diet and submitted to a high-carbohydrate test-meal the activity of amylase was increased by 50%. When the consumption of the high-lipid meal was associated with an intraduodenal load of 100 g of glucose all the enzyme activities were stimulated when compared to the effect of meal alone, but only the activity of amylase was significantly increased (+ 82%). In the juice of pigs adapted to a balanced diet and submitted to intraduodenal loads of 150 ml of water, 50 g of glucose, 50 g of maltose and 150 g of maltose, the enzyme activities remained almost constant with the load of water and 50 g of maltose but with 50 g of glucose and 150 g of maltose loads, amylase activity was increased by 20% and 30% respectively. It is suggested, that the exocrine pancreas of the pig adapts itself rapidly to the changes in the size of the intestinal pool of starch hydrolysis products.", "contents": "Pancreatic exocrine secretion in the pig following test meals of different composition and intra-duodenal loads of glucose and maltose. Eleven pigs were fitted with pancreatic and duodenal fistulae, and pancreatic juice collected permanently. Amylase, chymotrypsin, lipase and total proteins were determined in juice collected within 2 and 6 hours after different test-meals or intraduodenal loads of glucose and maltose. In the pancreatic juice of pigs adapted to a high-lipid diet and submitted to a high-carbohydrate test-meal the activity of amylase was increased by 50%. When the consumption of the high-lipid meal was associated with an intraduodenal load of 100 g of glucose all the enzyme activities were stimulated when compared to the effect of meal alone, but only the activity of amylase was significantly increased (+ 82%). In the juice of pigs adapted to a balanced diet and submitted to intraduodenal loads of 150 ml of water, 50 g of glucose, 50 g of maltose and 150 g of maltose, the enzyme activities remained almost constant with the load of water and 50 g of maltose but with 50 g of glucose and 150 g of maltose loads, amylase activity was increased by 20% and 30% respectively. It is suggested, that the exocrine pancreas of the pig adapts itself rapidly to the changes in the size of the intestinal pool of starch hydrolysis products."}
{"id": "PMID:478431", "title": "Amino acid transport in the exocrine pancreas. IV. Do glucagon or insulin mediate the in vivo effect of caerulein on amino acid transport and incorporation?", "content": "The direct in vitro effect of caerulein on pancreatic protein synthesis and amino acid transport has been investigated. In contrast to in vivo conditions we were unable to demonstrate any effect on alpha-aminoisobutyric acid and leucine uptake and on leucine incorporation usin rat pancreatic lobules. Insulin and glucagon were therefore examined as possible mediators for the in vivo effect of caerulein. Insulin (1--5 microM) slightly enhanced AIB uptake (16% but did not change uptake and incorporation of leucine. Glucagon (0.01--1 microM) was ineffective. Both islet hormones had no influence on the formation of cyclic GMP induced by secretagogue either in rat (40% increase) or in guinea pig lobules (500% increase). It seems unlikely that the two islet hormones exert any direct effect on the exocrine pancreas and thus could serve as mediators for the in vivo synthetic effect of caerulein.", "contents": "Amino acid transport in the exocrine pancreas. IV. Do glucagon or insulin mediate the in vivo effect of caerulein on amino acid transport and incorporation? The direct in vitro effect of caerulein on pancreatic protein synthesis and amino acid transport has been investigated. In contrast to in vivo conditions we were unable to demonstrate any effect on alpha-aminoisobutyric acid and leucine uptake and on leucine incorporation usin rat pancreatic lobules. Insulin and glucagon were therefore examined as possible mediators for the in vivo effect of caerulein. Insulin (1--5 microM) slightly enhanced AIB uptake (16% but did not change uptake and incorporation of leucine. Glucagon (0.01--1 microM) was ineffective. Both islet hormones had no influence on the formation of cyclic GMP induced by secretagogue either in rat (40% increase) or in guinea pig lobules (500% increase). It seems unlikely that the two islet hormones exert any direct effect on the exocrine pancreas and thus could serve as mediators for the in vivo synthetic effect of caerulein."}
{"id": "PMID:478432", "title": "Propranolol inhibits the in vitro conversion of thyroxine into triiodothyronine by isolated rat liver parenchymal cells.", "content": "A model for the in vitro study of the conversion of thyroxine into triiodothyronine using isolated rat liver parenchymal cells is described. Isolated liver cells (mean protein content 18 mg/ml) convert approximately 0.8% of 1.3 microM exogenously added T4 into T3 during thirty minutes incubation. Carbimazole (50 microM) has no effect on the conversion process, whereas propylthiouracil (50 microM) inhibits the conversion. The beta-adrenoceptor blocking agent propranolol lowers the conversion ratio when added in concentrations of 580 and 1160 microM, but has no inhibitory effect when 290 microM is added.", "contents": "Propranolol inhibits the in vitro conversion of thyroxine into triiodothyronine by isolated rat liver parenchymal cells. A model for the in vitro study of the conversion of thyroxine into triiodothyronine using isolated rat liver parenchymal cells is described. Isolated liver cells (mean protein content 18 mg/ml) convert approximately 0.8% of 1.3 microM exogenously added T4 into T3 during thirty minutes incubation. Carbimazole (50 microM) has no effect on the conversion process, whereas propylthiouracil (50 microM) inhibits the conversion. The beta-adrenoceptor blocking agent propranolol lowers the conversion ratio when added in concentrations of 580 and 1160 microM, but has no inhibitory effect when 290 microM is added."}
{"id": "PMID:478433", "title": "Radioimmunoassay for human parathyroid hormone for differentiation between patients with hypoparathyroidism, hyperparathyroidism and normals.", "content": "A RIA system for human PTH is presented using a goat antibody (Code name 017-spring-78) against C-terminal hPTH fragments, as well as a human PTH standard from hemodiafiltration of a hyperparathyroid patient. It proved to be useful for the differentiation not only between hyperparathyroid patients and normals, but even within the normal range and hypoparathyroid states.", "contents": "Radioimmunoassay for human parathyroid hormone for differentiation between patients with hypoparathyroidism, hyperparathyroidism and normals. A RIA system for human PTH is presented using a goat antibody (Code name 017-spring-78) against C-terminal hPTH fragments, as well as a human PTH standard from hemodiafiltration of a hyperparathyroid patient. It proved to be useful for the differentiation not only between hyperparathyroid patients and normals, but even within the normal range and hypoparathyroid states."}
{"id": "PMID:478435", "title": "The relationship between maximal oxygen uptake and glucose tolerance/insulin response ratio in normal young men.", "content": "Maximal oxygen uptake (VO2max.), glucose tolerance (K-value), and insulin response (IRI-area) were studied in seventeen young, non-obese, non-diabetic males. The ratio between K-value and IRI-area correlated significantly with VO2 max. (r = 0.70, p less than 0.01) also when differences in body fat mass were eliminated by partial correlation analysis (r = 0.56, p less than 0.05). Subjects with a high VO2 max. thus maintained a given glucose tolerance with a lower insulin response than did subjects in whom VO2 max. was low.", "contents": "The relationship between maximal oxygen uptake and glucose tolerance/insulin response ratio in normal young men. Maximal oxygen uptake (VO2max.), glucose tolerance (K-value), and insulin response (IRI-area) were studied in seventeen young, non-obese, non-diabetic males. The ratio between K-value and IRI-area correlated significantly with VO2 max. (r = 0.70, p less than 0.01) also when differences in body fat mass were eliminated by partial correlation analysis (r = 0.56, p less than 0.05). Subjects with a high VO2 max. thus maintained a given glucose tolerance with a lower insulin response than did subjects in whom VO2 max. was low."}
{"id": "PMID:478436", "title": "Modification by oral contraceptives in rat of 14C acetate incorporation into platelet lipids.", "content": "The in vitro incorporation of acetate 14C in platelets lipids was compared in control female rats (Gr. I) to rats treated for 4 days either by an oral contraceptive ethinyl oestradiol + lynestrenol (Gr. II), or by ethinyl oestradiol alone (Gr. III) or lynestrenol alone (Gr. IV). An increase of 43--45% in the incorporation of acetate could be observed in the two groups (II and III) which received ethinyl oestradiol, while the incorporation in group IV was similar to that of the controls. The lipid fractions of which the synthesis was the most considerably stimulated by the oestrogen treatment, were the neutral lipids as separated from the other lipids by TLC. In groups II and III the incorporation in cholesterol and cholesterol esters was increased by 8 fold and by 10 fold in the free fatty acid fraction. In these two groups, even in the phospholipid fractions PS + PI and PE, the radioactivity was significantly increased. The observed effect of the oral contraceptive studied here on platelet lipid synthesis in female rats, appears to be essentially due to the estrogens, since lynestrenol had only minimal effects in that respect.", "contents": "Modification by oral contraceptives in rat of 14C acetate incorporation into platelet lipids. The in vitro incorporation of acetate 14C in platelets lipids was compared in control female rats (Gr. I) to rats treated for 4 days either by an oral contraceptive ethinyl oestradiol + lynestrenol (Gr. II), or by ethinyl oestradiol alone (Gr. III) or lynestrenol alone (Gr. IV). An increase of 43--45% in the incorporation of acetate could be observed in the two groups (II and III) which received ethinyl oestradiol, while the incorporation in group IV was similar to that of the controls. The lipid fractions of which the synthesis was the most considerably stimulated by the oestrogen treatment, were the neutral lipids as separated from the other lipids by TLC. In groups II and III the incorporation in cholesterol and cholesterol esters was increased by 8 fold and by 10 fold in the free fatty acid fraction. In these two groups, even in the phospholipid fractions PS + PI and PE, the radioactivity was significantly increased. The observed effect of the oral contraceptive studied here on platelet lipid synthesis in female rats, appears to be essentially due to the estrogens, since lynestrenol had only minimal effects in that respect."}
{"id": "PMID:478437", "title": "Substance P plasma levels in pregnancy and in various clinical disorders.", "content": "Using radioimmunoassay, immunoreactive SP (iSP) has been measured in the plasma of 162 hospital patients with various disorders and in the plasma of 67 pregnant women. In pregnancy, iSP was undetectable in 40% women as compared to 12% in other hospital patients. The mean iSP plasma level in pregnancy was 37.8 +/- 4.6 (SEM) as compared to 77.1 +/- 4.9 (SEM) pg/ml in other hospital patients. The results support earlier observations based on bioassay, suggesting that the blood of pregnant women contains higher concentrations of a SP-inactivating factor. Of the hospital patients, elevated levels of iSP were found in patients with chronic leukaemia, in one patient with a basaloid carcinoma of the anus, and in one patient with toxic liver damage and pancreatic insufficiency. No correlation was found between thyroid function and iSP plasma levels. ISP plasma levels in various gastrointestinal disorders were similar to those found in normal subjects.", "contents": "Substance P plasma levels in pregnancy and in various clinical disorders. Using radioimmunoassay, immunoreactive SP (iSP) has been measured in the plasma of 162 hospital patients with various disorders and in the plasma of 67 pregnant women. In pregnancy, iSP was undetectable in 40% women as compared to 12% in other hospital patients. The mean iSP plasma level in pregnancy was 37.8 +/- 4.6 (SEM) as compared to 77.1 +/- 4.9 (SEM) pg/ml in other hospital patients. The results support earlier observations based on bioassay, suggesting that the blood of pregnant women contains higher concentrations of a SP-inactivating factor. Of the hospital patients, elevated levels of iSP were found in patients with chronic leukaemia, in one patient with a basaloid carcinoma of the anus, and in one patient with toxic liver damage and pancreatic insufficiency. No correlation was found between thyroid function and iSP plasma levels. ISP plasma levels in various gastrointestinal disorders were similar to those found in normal subjects."}
{"id": "PMID:478438", "title": "Studies of growth hormone secretion in juvenile diabetes.", "content": "To further investigate the GH secretion in juvenile diabetics, blood glucose (BG) and plasma growth hormone (GH) were determined during controlled exercise performed in basal condition and under glucose infusion, in 7 controls and 22 juvenile diabetics aged 12--35 years, 10 of them with fundal vascular lesions. In controls, glucose infusion significantly lowered the exercise induced GH rise observed under basal conditions. In diabetics, under basal conditions, diabetics with low basal BG (BG less than 100 mg/100ml) had higher GH secretion than those with high basal BG (BG greater than 140 mg/100 ml; p less than 0.05). Under glucose infusion, diabetics with normal BG peak values (not different from controls: BG = 284 +/- (SK) 45 mg/100 ml) had significantly higher plasma GH levels than controls (p less than 0.01). In contrast, in diabetics with BG peak value higher than controls (BG greater than 374 ng/100 ml), plasma GH levels were not different from control values. This study indicates that exercise induced GH secretion in diabetics is mainly related to actual BG levels. Furthermore, we found no relation between the magnitude of GH secretion and the presence of retinopathy in diabetics.", "contents": "Studies of growth hormone secretion in juvenile diabetes. To further investigate the GH secretion in juvenile diabetics, blood glucose (BG) and plasma growth hormone (GH) were determined during controlled exercise performed in basal condition and under glucose infusion, in 7 controls and 22 juvenile diabetics aged 12--35 years, 10 of them with fundal vascular lesions. In controls, glucose infusion significantly lowered the exercise induced GH rise observed under basal conditions. In diabetics, under basal conditions, diabetics with low basal BG (BG less than 100 mg/100ml) had higher GH secretion than those with high basal BG (BG greater than 140 mg/100 ml; p less than 0.05). Under glucose infusion, diabetics with normal BG peak values (not different from controls: BG = 284 +/- (SK) 45 mg/100 ml) had significantly higher plasma GH levels than controls (p less than 0.01). In contrast, in diabetics with BG peak value higher than controls (BG greater than 374 ng/100 ml), plasma GH levels were not different from control values. This study indicates that exercise induced GH secretion in diabetics is mainly related to actual BG levels. Furthermore, we found no relation between the magnitude of GH secretion and the presence of retinopathy in diabetics."}
{"id": "PMID:478439", "title": "Adenohypophyseal hormone response to chronic stress in dexamethasone-treated rats.", "content": "The influence of dexamethasone treatment on the basal values of corticosterone, GH, prolactin (PRL), LH and FSH, as well as on the adenohypophyseal hormone response to chronic stress was studied in female rats. Dexamethasone acetate (25 micrograms/100 b.w.), given by gavage twice daily for 10 days, decreased the resting plasma levels of corticosterone, GH, LH and PRL, whereas the FSH titers remained normal. The secretion of ACTH (evaluated indirectly through corticosterone concentrations) and of GH appeared to be most sensitive to the suppressive effect of dexamethasone. The same hormonal response pattern was induced by 8 h of daily immobilization for 10 days, except that ACTH release was enhanced and the plasma LH titers dropped more drastically. Dexamethasone administration in combination with restraint did not alter the characteristic hormonal profile of chronic stress, despite the fact that ACTH secretion was completely blocked. These data suggest that the inhibition of PRL, LH and GH secretion following severe, chronic stress is not causally related to the sustained elevation of plasma ACTH.", "contents": "Adenohypophyseal hormone response to chronic stress in dexamethasone-treated rats. The influence of dexamethasone treatment on the basal values of corticosterone, GH, prolactin (PRL), LH and FSH, as well as on the adenohypophyseal hormone response to chronic stress was studied in female rats. Dexamethasone acetate (25 micrograms/100 b.w.), given by gavage twice daily for 10 days, decreased the resting plasma levels of corticosterone, GH, LH and PRL, whereas the FSH titers remained normal. The secretion of ACTH (evaluated indirectly through corticosterone concentrations) and of GH appeared to be most sensitive to the suppressive effect of dexamethasone. The same hormonal response pattern was induced by 8 h of daily immobilization for 10 days, except that ACTH release was enhanced and the plasma LH titers dropped more drastically. Dexamethasone administration in combination with restraint did not alter the characteristic hormonal profile of chronic stress, despite the fact that ACTH secretion was completely blocked. These data suggest that the inhibition of PRL, LH and GH secretion following severe, chronic stress is not causally related to the sustained elevation of plasma ACTH."}
{"id": "PMID:478440", "title": "Long-term cimetidine does not alter serum prolactin.", "content": "The effect of repeated cimetidine ingestion on serum prolactin values was studied prospectively in 17 men with proven duodenal ulcers. These patients received 400 mg of cimetidine twice daily for 12 weeks but showed no alteration in their mean serum prolactin levels. Cimetidine-induced hyperprolactinaemia is not the explanation for the development of gynaecomastia in men exposed to this drug.", "contents": "Long-term cimetidine does not alter serum prolactin. The effect of repeated cimetidine ingestion on serum prolactin values was studied prospectively in 17 men with proven duodenal ulcers. These patients received 400 mg of cimetidine twice daily for 12 weeks but showed no alteration in their mean serum prolactin levels. Cimetidine-induced hyperprolactinaemia is not the explanation for the development of gynaecomastia in men exposed to this drug."}
{"id": "PMID:478441", "title": "Effect of oral contraceptive used by technician on sensitivity of progesterone radioimmunoassay.", "content": "Gonadotropin and steroid radioimmunoassays (RIAs) have been run in our laboratory for the past 6 years. Recently, the progesterone RIA was unpredictable when performed by one technician. After checking the mechanics and reagents, it was discovered that the technician had changed from Ortho Novum 1 + 50 to Ovral about the time the RIA became erratic. Standard curves of progesterone RIAs performed while the technician was taking either Ortho Novum 1 + 50 or Ovral, or on no contraceptive are reported. The data suggest that a probably exhaled metabolic product of norgestrel, the synthetic progestin in Ovral, interferes with the progesterone RIA and indicate an additional variable investigators must eliminate in performing RIAs.", "contents": "Effect of oral contraceptive used by technician on sensitivity of progesterone radioimmunoassay. Gonadotropin and steroid radioimmunoassays (RIAs) have been run in our laboratory for the past 6 years. Recently, the progesterone RIA was unpredictable when performed by one technician. After checking the mechanics and reagents, it was discovered that the technician had changed from Ortho Novum 1 + 50 to Ovral about the time the RIA became erratic. Standard curves of progesterone RIAs performed while the technician was taking either Ortho Novum 1 + 50 or Ovral, or on no contraceptive are reported. The data suggest that a probably exhaled metabolic product of norgestrel, the synthetic progestin in Ovral, interferes with the progesterone RIA and indicate an additional variable investigators must eliminate in performing RIAs."}
{"id": "PMID:478442", "title": "Counteraction of testosterone-induced suppression of the pituitary-ovarian axis in rats by flutamide.", "content": "Daily administration of 100 micrograms of testosterone to unilaterally ovariectomized rats for 18 days caused a significant decrease in compensatory ovarian hypertrophy. The number of corpora lutea was markedly reduced and many cystic follicles were noticeable. Administration of graded doses (0.5, 1 and 3 mg daily) of flutamide over the same period did not cause any significant change in the weight and histology of the ovary in untreated unilaterally ovariectomized animals. However, treatment with the same doses of flutamide prevented the changes observed in the reamining ovary of testosterone-treated animals.", "contents": "Counteraction of testosterone-induced suppression of the pituitary-ovarian axis in rats by flutamide. Daily administration of 100 micrograms of testosterone to unilaterally ovariectomized rats for 18 days caused a significant decrease in compensatory ovarian hypertrophy. The number of corpora lutea was markedly reduced and many cystic follicles were noticeable. Administration of graded doses (0.5, 1 and 3 mg daily) of flutamide over the same period did not cause any significant change in the weight and histology of the ovary in untreated unilaterally ovariectomized animals. However, treatment with the same doses of flutamide prevented the changes observed in the reamining ovary of testosterone-treated animals."}
{"id": "PMID:478443", "title": "Estradiol metabolism by liver homogenates and microsomes of normal and cirrhotic male rats.", "content": "Incubation experiments show that homogenates and microsomes obtained from CCl4-induced cirrhotic livers of male rats metabolize an estradiol load at a much slower rate than preparations from normal livers. The decreased metabolic capacity results in a slower disappearance of estradiol from the incubation medium, and in a slower transformation of metabolized estradiol to polar extractable and to highly polar nonextractable metabolites.", "contents": "Estradiol metabolism by liver homogenates and microsomes of normal and cirrhotic male rats. Incubation experiments show that homogenates and microsomes obtained from CCl4-induced cirrhotic livers of male rats metabolize an estradiol load at a much slower rate than preparations from normal livers. The decreased metabolic capacity results in a slower disappearance of estradiol from the incubation medium, and in a slower transformation of metabolized estradiol to polar extractable and to highly polar nonextractable metabolites."}
{"id": "PMID:478448", "title": "Proposition 13: experience in one community mental health center.", "content": "The impact of Proposition 13 on one community mental health center in California is described. Although the center has had no layoffs as a result of its passage, the measure has had a serious effect on staff morale, which was already at a low point because of pre-existing budgetary constrictions that led to staff reductions despite increased demands for services. It has also generated several changes in treatment programs designed to reduce overhead costs and make more efficient use of staff time. The author emphasizes that because of the supplemental funds made available by the state from its surplus during fiscal year 1978-79, the full impact of Proposition 13 on local service programs is still to be felt.", "contents": "Proposition 13: experience in one community mental health center. The impact of Proposition 13 on one community mental health center in California is described. Although the center has had no layoffs as a result of its passage, the measure has had a serious effect on staff morale, which was already at a low point because of pre-existing budgetary constrictions that led to staff reductions despite increased demands for services. It has also generated several changes in treatment programs designed to reduce overhead costs and make more efficient use of staff time. The author emphasizes that because of the supplemental funds made available by the state from its surplus during fiscal year 1978-79, the full impact of Proposition 13 on local service programs is still to be felt."}
{"id": "PMID:478449", "title": "Psychiatrists and primary physicians: collaborative learning experiences in delivering primary care.", "content": "Psychiatric and primary care residents can benefit from taking part of their training together in a primary care setting such as a neighborhood health center or a health maintenance organization. The training in such organized settings can best focus on the comprehensive appraisal of patients' multiple health, mental health, and health-related social service needs; the coordination of care to devise nonfragmented treatment plans for multi-problem patients; the effective collaboration between health generalists and mental health specialists; and the efficient use of limited resources to meet a population's health care needs. Such a coordinated training experience also helps overcome interprofessional antagonisms that often inhibit collaboration between psychiatrists and other physicians.", "contents": "Psychiatrists and primary physicians: collaborative learning experiences in delivering primary care. Psychiatric and primary care residents can benefit from taking part of their training together in a primary care setting such as a neighborhood health center or a health maintenance organization. The training in such organized settings can best focus on the comprehensive appraisal of patients' multiple health, mental health, and health-related social service needs; the coordination of care to devise nonfragmented treatment plans for multi-problem patients; the effective collaboration between health generalists and mental health specialists; and the efficient use of limited resources to meet a population's health care needs. Such a coordinated training experience also helps overcome interprofessional antagonisms that often inhibit collaboration between psychiatrists and other physicians."}
{"id": "PMID:478451", "title": "Private-sector services for low-income psychiatric outpatients. D.C. Institute of Mental Hygiene Washington, D.C.", "content": "A community-based, nonprofit outpatient clinic in Washington, D.C., has been successful in using the medical model to provide high-quality services to low-income patients. In 13 years it has grown from five staff members and 17 patients to a part-time staff of 74 and a patient population of 1500. Hours of direct service have increased from 1000 to more than 40,000 per year. The clinic has matched the services provided in the city's public mental health clinics at a fraction of the cost. It receives no direct federal funding and only minimal assistance from state and local governments. It exemplifies the contribution the private sector can make in creatively serving the economically disadvantaged. Fiscal reality and patient demand emphasize the need for a strong and ongoing partnership between the private and public sectors if that population is to be served.", "contents": "Private-sector services for low-income psychiatric outpatients. D.C. Institute of Mental Hygiene Washington, D.C. A community-based, nonprofit outpatient clinic in Washington, D.C., has been successful in using the medical model to provide high-quality services to low-income patients. In 13 years it has grown from five staff members and 17 patients to a part-time staff of 74 and a patient population of 1500. Hours of direct service have increased from 1000 to more than 40,000 per year. The clinic has matched the services provided in the city's public mental health clinics at a fraction of the cost. It receives no direct federal funding and only minimal assistance from state and local governments. It exemplifies the contribution the private sector can make in creatively serving the economically disadvantaged. Fiscal reality and patient demand emphasize the need for a strong and ongoing partnership between the private and public sectors if that population is to be served."}
{"id": "PMID:478457", "title": "New reporting system aids epidemiologists.", "content": "The feedback loop reporting system lifts the burden of reporting communicable diseases from the physician's shoulders and ensures more efficient, and more frequent, reporting.", "contents": "New reporting system aids epidemiologists. The feedback loop reporting system lifts the burden of reporting communicable diseases from the physician's shoulders and ensures more efficient, and more frequent, reporting."}
{"id": "PMID:478458", "title": "Area agencies ensure success of disaster drill.", "content": "Salem County (NJ) Memorial Hospital cooperated in an areawide disaster drill and found that it took large doses of planning and cooperation to coordinate the effort.", "contents": "Area agencies ensure success of disaster drill. Salem County (NJ) Memorial Hospital cooperated in an areawide disaster drill and found that it took large doses of planning and cooperation to coordinate the effort."}
{"id": "PMID:478459", "title": "Food service researched in special project.", "content": "Regional research project, funded by the Department of Agriculture and staffed by specialists, is conducting studies of food quality, food sanitation, and energy conservation.", "contents": "Food service researched in special project. Regional research project, funded by the Department of Agriculture and staffed by specialists, is conducting studies of food quality, food sanitation, and energy conservation."}
{"id": "PMID:478460", "title": "Pharmacists' clinical role expanded.", "content": "A hospital used management engineering to achieve more effective utilization of its pharmacy staff so that pharmacists' clinical role could be expanded without increases in staff or costs.", "contents": "Pharmacists' clinical role expanded. A hospital used management engineering to achieve more effective utilization of its pharmacy staff so that pharmacists' clinical role could be expanded without increases in staff or costs."}
{"id": "PMID:478473", "title": "Energy situation is precarious.", "content": "Given the uncertain future that lies ahead for meeting the nation's energy needs, many hospitals are assuming a posture of vigilance and preparedness. If they are not, they should be, experts say.", "contents": "Energy situation is precarious. Given the uncertain future that lies ahead for meeting the nation's energy needs, many hospitals are assuming a posture of vigilance and preparedness. If they are not, they should be, experts say."}
{"id": "PMID:478474", "title": "What are the antitrust implications of shared purchasing for hospitals?", "content": "Although it can be argued that shared purchasing arrangements are lawful in most circumstances, competent legal counsel should examine the agreements to be sure. Courts are likely to examine such arrangements to distinguish managerial reform from anticompetitive actions.", "contents": "What are the antitrust implications of shared purchasing for hospitals? Although it can be argued that shared purchasing arrangements are lawful in most circumstances, competent legal counsel should examine the agreements to be sure. Courts are likely to examine such arrangements to distinguish managerial reform from anticompetitive actions."}
{"id": "PMID:478475", "title": "The possible dream: the Navajo Nation Health Foundation.", "content": "The dream of high-quality health care for the Navajo began in Ganado, AZ, some 70 years ago. Now it has gone a step further through the work of the only hospital and clinic system in the United States that is owned and operated by American Indians.", "contents": "The possible dream: the Navajo Nation Health Foundation. The dream of high-quality health care for the Navajo began in Ganado, AZ, some 70 years ago. Now it has gone a step further through the work of the only hospital and clinic system in the United States that is owned and operated by American Indians."}
{"id": "PMID:478476", "title": "It's not that kind of a job.", "content": "The duties of the corporation president and the hospital administrator are less publicly visible than those of the senator and the physician, but that doesn't make them less valuable.", "contents": "It's not that kind of a job. The duties of the corporation president and the hospital administrator are less publicly visible than those of the senator and the physician, but that doesn't make them less valuable."}
{"id": "PMID:478477", "title": "Hypertension clinic proves effective.", "content": "Hypertension can best be combatted through patient education and early detection. A screening clinic can be an excellent way of reaching the public if follow-up is diligent.", "contents": "Hypertension clinic proves effective. Hypertension can best be combatted through patient education and early detection. A screening clinic can be an excellent way of reaching the public if follow-up is diligent."}
{"id": "PMID:478481", "title": "Center for Life provides accessible maternity care.", "content": "Providing good, comprehensive, accessible maternity care to the underserved can help solve a hospital's declining OB census and can support a pro-life philosophy.", "contents": "Center for Life provides accessible maternity care. Providing good, comprehensive, accessible maternity care to the underserved can help solve a hospital's declining OB census and can support a pro-life philosophy."}
{"id": "PMID:478482", "title": "Hospital buildings have few alternative uses.", "content": "A year ago HEW published guidelines regulating hospital beds and occupancy rates. Reluctant to close their doors, administrators have sought conversion alternatives, most of them impractical and economically impossible. A go-slow attitude is recommended, as population increases will dictate hospitals' proper response.", "contents": "Hospital buildings have few alternative uses. A year ago HEW published guidelines regulating hospital beds and occupancy rates. Reluctant to close their doors, administrators have sought conversion alternatives, most of them impractical and economically impossible. A go-slow attitude is recommended, as population increases will dictate hospitals' proper response."}
{"id": "PMID:478483", "title": "Responding to consumer demand: a study of alternative birth centers.", "content": "Responding to consumer demands, many hospitals are setting up ABCs for couples who desire a more self-directed, family-centered experience. Such programs require that administrators and staff anticipate potential problems for personnel and clients.", "contents": "Responding to consumer demand: a study of alternative birth centers. Responding to consumer demands, many hospitals are setting up ABCs for couples who desire a more self-directed, family-centered experience. Such programs require that administrators and staff anticipate potential problems for personnel and clients."}
{"id": "PMID:478484", "title": "Analyzing the hospice market.", "content": "Hospitals should base decisions about hospice services on need rather than on subjective feelings. Marketing methods can be used to identify the potential population for hospice care.", "contents": "Analyzing the hospice market. Hospitals should base decisions about hospice services on need rather than on subjective feelings. Marketing methods can be used to identify the potential population for hospice care."}
{"id": "PMID:478485", "title": "Storeroom procedures that conserve assets.", "content": "As nonlabor costs in health care increase disproportionately, changes in storeroom operations will become an important cost containment tool.", "contents": "Storeroom procedures that conserve assets. As nonlabor costs in health care increase disproportionately, changes in storeroom operations will become an important cost containment tool."}
{"id": "PMID:478487", "title": "The 1979 CHA survey of pastoral care programs.", "content": "Pastoral care programs have grown and matured in the 10 years since The Catholic Hospital Association and the National Association of Catholic Chaplains issued their \"Guidelines for Establishing a Department of Religion in a Catholic-Sponsored Hospital.\" CHA's 1979 survey of 370 institutions points out that while pastoral care departments are recognized as important, budgets for personnel and programs are unrealistic. The shortage of pastoral care personnel is an acute challenge to Catholic facilities, and alternative staffing methods must be sought.", "contents": "The 1979 CHA survey of pastoral care programs. Pastoral care programs have grown and matured in the 10 years since The Catholic Hospital Association and the National Association of Catholic Chaplains issued their \"Guidelines for Establishing a Department of Religion in a Catholic-Sponsored Hospital.\" CHA's 1979 survey of 370 institutions points out that while pastoral care departments are recognized as important, budgets for personnel and programs are unrealistic. The shortage of pastoral care personnel is an acute challenge to Catholic facilities, and alternative staffing methods must be sought."}
{"id": "PMID:478518", "title": "Recognition mechanisms in infectious disease.", "content": "Recent studies have begun to elucidate the nature of pathogen-host recognition mechanisms. Not only is our understanding of the nature of infection thereby growing, but there is promise of an entirely new approach to treatment--by inhibiting or preventing the contact between pathogen and target cell, the first step in the infectious process. Models have largely been elucidated for shigella infections and cholera.", "contents": "Recognition mechanisms in infectious disease. Recent studies have begun to elucidate the nature of pathogen-host recognition mechanisms. Not only is our understanding of the nature of infection thereby growing, but there is promise of an entirely new approach to treatment--by inhibiting or preventing the contact between pathogen and target cell, the first step in the infectious process. Models have largely been elucidated for shigella infections and cholera."}
{"id": "PMID:478519", "title": "Systemic lupus erythematosus.", "content": "SLE often presents such a confusing clinical picture that diagnosis is long delayed, and it remains a disease of unknown cause. But early diagnosis and carefully planned therapy can enable many patients to lead nearly normal lives. Recent advances are outlined.", "contents": "Systemic lupus erythematosus. SLE often presents such a confusing clinical picture that diagnosis is long delayed, and it remains a disease of unknown cause. But early diagnosis and carefully planned therapy can enable many patients to lead nearly normal lives. Recent advances are outlined."}
{"id": "PMID:478520", "title": "Malabsorption in the elderly.", "content": "Age-related deficiencies in gastrointestinal function do occur, but the evidence suggests that their contribution to malabsorption syndromes is usually trivial. Symptoms of malabsorption in elderly patients therefore probably do not simply reflect \"old age,\" but some underlying problem(s). An orderly approach to diagnosis, emphasizing disorders likely to be present in the geriatric population, can lead to effective treatment.", "contents": "Malabsorption in the elderly. Age-related deficiencies in gastrointestinal function do occur, but the evidence suggests that their contribution to malabsorption syndromes is usually trivial. Symptoms of malabsorption in elderly patients therefore probably do not simply reflect \"old age,\" but some underlying problem(s). An orderly approach to diagnosis, emphasizing disorders likely to be present in the geriatric population, can lead to effective treatment."}
{"id": "PMID:478521", "title": "Skeletal scintigraphy in the diagnosis of acute osteomyelitis.", "content": "Bone scans detect osteomyelitis earlier, sometimes several days earlier, than x-rays. Prompt institution of therapy, before significant bone damage has developed, thus becomes possible. A positive scintigram is, however, nonspecific, and firm diagnosis requires radiologic and other confirmation. Moreover, because the technique is so new, many open questions remain both about its optimal utilization and about its potential.", "contents": "Skeletal scintigraphy in the diagnosis of acute osteomyelitis. Bone scans detect osteomyelitis earlier, sometimes several days earlier, than x-rays. Prompt institution of therapy, before significant bone damage has developed, thus becomes possible. A positive scintigram is, however, nonspecific, and firm diagnosis requires radiologic and other confirmation. Moreover, because the technique is so new, many open questions remain both about its optimal utilization and about its potential."}
{"id": "PMID:478534", "title": "The quantitative analysis of polymorphism on human chromosomes 1, 9, 16, and Y. II. Comparison of the C segments in male and female individuals (group characteristics).", "content": "Comparative analysis of the polymorphism of C segments on chromosomes 1, 9, 16, and Y was conducted in 50 normal boys and 50 normal girls. Quantitative methods revealed that the mean lengths of C segments, their variability, and their distribution on the chromosomes mentioned are quite identical in the two groups. Methodological problems related to the study of chromosome polymorphism are discussed.", "contents": "The quantitative analysis of polymorphism on human chromosomes 1, 9, 16, and Y. II. Comparison of the C segments in male and female individuals (group characteristics). Comparative analysis of the polymorphism of C segments on chromosomes 1, 9, 16, and Y was conducted in 50 normal boys and 50 normal girls. Quantitative methods revealed that the mean lengths of C segments, their variability, and their distribution on the chromosomes mentioned are quite identical in the two groups. Methodological problems related to the study of chromosome polymorphism are discussed."}
{"id": "PMID:478535", "title": "The quantitative analysis of polymorphism on human chromosomes 1, 9, 16, and Y. III. Study of relationships of C segments' lengths in individual karyotypes.", "content": "The correlation relationship of the lengths of C segments on chromosomes 1, 9, 16, and Y was studied in a group of normal boys and girls (50 boys and 50 girls). No correlation was found between the C segment lengths, either on homologs or on chromosomes of various pairs. This confirms the idea of random combinations of the said chromosomes with C segments of different lengths in an individual karyotype.", "contents": "The quantitative analysis of polymorphism on human chromosomes 1, 9, 16, and Y. III. Study of relationships of C segments' lengths in individual karyotypes. The correlation relationship of the lengths of C segments on chromosomes 1, 9, 16, and Y was studied in a group of normal boys and girls (50 boys and 50 girls). No correlation was found between the C segment lengths, either on homologs or on chromosomes of various pairs. This confirms the idea of random combinations of the said chromosomes with C segments of different lengths in an individual karyotype."}
{"id": "PMID:478536", "title": "Short rib-polydactyly syndrome, Majewski type, in two male siblings.", "content": "Two male siblings with a short rib-polydactyly syndrome, Majewski type (Majewski syndrome), are reported. To our knowledge, the present report is the first one of siblings each confirmed to be affected by Majewski syndrome, and it may therefore contribute to establishing the mode of inheritance.", "contents": "Short rib-polydactyly syndrome, Majewski type, in two male siblings. Two male siblings with a short rib-polydactyly syndrome, Majewski type (Majewski syndrome), are reported. To our knowledge, the present report is the first one of siblings each confirmed to be affected by Majewski syndrome, and it may therefore contribute to establishing the mode of inheritance."}
{"id": "PMID:478537", "title": "Partial trisomy for the long arm of chromosome 7 due to familial balanced translocation.", "content": "A partial trisomy for the distal segment of the long arm of chromosome 7 (bands q32 replaced by qter) was observed in a severely retarded child with somatic and CNS anomalies. The phenotypically normal father and paternal grandmother had a balanced reciprocal translocation between the long arm of a chromosome 2 and the long arm of a chromosome 7: 46,XX-XY,t(2;7) (q37;q32). The clinical features of the child at birth and at the ages of 5 months and 2 years are compared with those previously reported in cases of partial trisomy 7q.", "contents": "Partial trisomy for the long arm of chromosome 7 due to familial balanced translocation. A partial trisomy for the distal segment of the long arm of chromosome 7 (bands q32 replaced by qter) was observed in a severely retarded child with somatic and CNS anomalies. The phenotypically normal father and paternal grandmother had a balanced reciprocal translocation between the long arm of a chromosome 2 and the long arm of a chromosome 7: 46,XX-XY,t(2;7) (q37;q32). The clinical features of the child at birth and at the ages of 5 months and 2 years are compared with those previously reported in cases of partial trisomy 7q."}
{"id": "PMID:478538", "title": "Simultaneous staining of sister chromatid exchanges and Q-bands in human chromosomes after treatment with methyl methane sulphonate, quinacrine mustard, and quinacrine.", "content": "Human peripheral lymphocyte chromosomes were stained simultaneously for sister chromatid exchanges (SCEs) and Q-banding. No effect of treatment with MMS, QM, and Q on the distribution of SCEs in chromosomes was found compared with controls. The SCEs were distributed between chromosomes roughly according to metaphase length, with the shorter chromosomes underrepresented. The majority of SCEs were located to pale bands, while a few occurred in bright bands and at interfaces between pale and bright bands. A greater frequency than expected of SCEs had occurred at identical sites in homologous chromosomes. This frequency was significantly increased after treatment with MMS.", "contents": "Simultaneous staining of sister chromatid exchanges and Q-bands in human chromosomes after treatment with methyl methane sulphonate, quinacrine mustard, and quinacrine. Human peripheral lymphocyte chromosomes were stained simultaneously for sister chromatid exchanges (SCEs) and Q-banding. No effect of treatment with MMS, QM, and Q on the distribution of SCEs in chromosomes was found compared with controls. The SCEs were distributed between chromosomes roughly according to metaphase length, with the shorter chromosomes underrepresented. The majority of SCEs were located to pale bands, while a few occurred in bright bands and at interfaces between pale and bright bands. A greater frequency than expected of SCEs had occurred at identical sites in homologous chromosomes. This frequency was significantly increased after treatment with MMS."}
{"id": "PMID:478539", "title": "Chromosome lesions in amniotic fluid cell cultures.", "content": "The frequencies of chromosome lesions were determined on 3537 mitoses in samples of varying sizes from cultures of 25 amniotic fluid specimens taken from patients at cytogenetic risk. The average percentage values of aberrant cells, including and excluding gaps, were 12.5 and 4.9, respectively. The corresponding values for fibroblasts and peripheral blood lymphocytes from normal adult donors, calculated under the same laboratory conditions, were 5.0 (including gaps) and 2.4 (excluding gaps) and 2.4 (including gaps) and 1.0 (excluding gaps), respectively. The hypothesis of a correlation between the increased incidence of chromosome lesions and the occurrence of abnormal karyotypes in amniotic fluid cell cultures is discussed.", "contents": "Chromosome lesions in amniotic fluid cell cultures. The frequencies of chromosome lesions were determined on 3537 mitoses in samples of varying sizes from cultures of 25 amniotic fluid specimens taken from patients at cytogenetic risk. The average percentage values of aberrant cells, including and excluding gaps, were 12.5 and 4.9, respectively. The corresponding values for fibroblasts and peripheral blood lymphocytes from normal adult donors, calculated under the same laboratory conditions, were 5.0 (including gaps) and 2.4 (excluding gaps) and 2.4 (including gaps) and 1.0 (excluding gaps), respectively. The hypothesis of a correlation between the increased incidence of chromosome lesions and the occurrence of abnormal karyotypes in amniotic fluid cell cultures is discussed."}
{"id": "PMID:478540", "title": "Intracellular phenylalanine and tyrosine concentrations in 19 heterozygotes for phenylketonuria (PKU) and 26 normals. Do the higher values in heterozygotes explain their lowered intellectual level?", "content": "Intracellular phenylalanine and tyrosine was determined in lymphocytes of 10 heterozygotes (parents) for PKU and in 26 randomly collected apparently normal persons. In cells from the heterozygotes the concentrations of both phenylalanine and tyrosine were higher than in those from the normals, the difference being statistically highly significant. It is argued that this could be responsible for the slight, though statistically significant, intellectual inferiority of heterozygotes for PKU.", "contents": "Intracellular phenylalanine and tyrosine concentrations in 19 heterozygotes for phenylketonuria (PKU) and 26 normals. Do the higher values in heterozygotes explain their lowered intellectual level? Intracellular phenylalanine and tyrosine was determined in lymphocytes of 10 heterozygotes (parents) for PKU and in 26 randomly collected apparently normal persons. In cells from the heterozygotes the concentrations of both phenylalanine and tyrosine were higher than in those from the normals, the difference being statistically highly significant. It is argued that this could be responsible for the slight, though statistically significant, intellectual inferiority of heterozygotes for PKU."}
{"id": "PMID:478541", "title": "Genetic variation in parotid basic proteins (Pb) in the Bozo (Mali, West Africa).", "content": "The frequency of variants in the Pb system was studied in 71 individuals from the Bozo tribe in the Bani-Niger area (Mali, West Africa), in 25 male students from different African countries, and in 110 Dutch students. The frequency of the Pb2 allele was 0.20 in the Bozo and 0.00 in the Dutch population. A comparison is made with the results of a study among American blacks and with a Japanese study. The Pb 2--2 phenotypes observed in this study favour the hypothesis that the Pb-2 c protein is derived from a larger precursor in a manner analogous to the generation of the Pb-1 b protein from Pb-1 protein e.", "contents": "Genetic variation in parotid basic proteins (Pb) in the Bozo (Mali, West Africa). The frequency of variants in the Pb system was studied in 71 individuals from the Bozo tribe in the Bani-Niger area (Mali, West Africa), in 25 male students from different African countries, and in 110 Dutch students. The frequency of the Pb2 allele was 0.20 in the Bozo and 0.00 in the Dutch population. A comparison is made with the results of a study among American blacks and with a Japanese study. The Pb 2--2 phenotypes observed in this study favour the hypothesis that the Pb-2 c protein is derived from a larger precursor in a manner analogous to the generation of the Pb-1 b protein from Pb-1 protein e."}
{"id": "PMID:478542", "title": "Partial trisomy 17q. Karyotype: 46,XY,der(21),t(17;21)(q22;p13).", "content": "A 15-year-old deeply mentally retarded male is described with partial distal 17q trisomy (17q22 replaced by 17qter),as the result of a de novo 17q/21p translocation. Differential Ag-staining showed that the satellites of chromosome 21 were included in the translocation chromosome.", "contents": "Partial trisomy 17q. Karyotype: 46,XY,der(21),t(17;21)(q22;p13). A 15-year-old deeply mentally retarded male is described with partial distal 17q trisomy (17q22 replaced by 17qter),as the result of a de novo 17q/21p translocation. Differential Ag-staining showed that the satellites of chromosome 21 were included in the translocation chromosome."}
{"id": "PMID:478550", "title": "C3 polymorphism in some Indian populations.", "content": "The distribution of C'3 phenotypes was studied in one tribal and three urban populations from India. The C'3F gene was found low in frequency compared to European and West Asian populations. Quantitatively also, the concentration of the C3 component in the Indian region was found significantly low to the European and West Asian populations reported previously.", "contents": "C3 polymorphism in some Indian populations. The distribution of C'3 phenotypes was studied in one tribal and three urban populations from India. The C'3F gene was found low in frequency compared to European and West Asian populations. Quantitatively also, the concentration of the C3 component in the Indian region was found significantly low to the European and West Asian populations reported previously."}
{"id": "PMID:478551", "title": "Genetic linkage analysis of hemoglobin variants in 175 families.", "content": "Linkage analysis using the LIPED program developed by Ott for the analysis of whole family data was performed on 175 families with variants of the hemoglobin beta- or gamma-chain. Comparison of our results with those in the literature indicate that there is little evidence for linkage with any of the currently used markers, although there is considerable heterogeneity in lod scores between sexes for the Duffy and MNS blood groups. It is suggested that the most successful approach in the future will be to analyze markers known to be localized on chromosomes which have been indicated as likely sites of the beta-chain locus from hybridization studies.", "contents": "Genetic linkage analysis of hemoglobin variants in 175 families. Linkage analysis using the LIPED program developed by Ott for the analysis of whole family data was performed on 175 families with variants of the hemoglobin beta- or gamma-chain. Comparison of our results with those in the literature indicate that there is little evidence for linkage with any of the currently used markers, although there is considerable heterogeneity in lod scores between sexes for the Duffy and MNS blood groups. It is suggested that the most successful approach in the future will be to analyze markers known to be localized on chromosomes which have been indicated as likely sites of the beta-chain locus from hybridization studies."}
{"id": "PMID:478552", "title": "Polymorphism of glyoxalase I in Vienna.", "content": "Phenotype and gene frequencies of the GLO I polymorphism in Vienna are given. No exception to the postulated rule of inheritance could be found in 23 families with 51 children and 132 mother-child pairs. Linkage with the HLA system is confirmed, but no linkage disequilibrium between GLO alleles and HLA-A, B, C genes was detected. The use of the GLO I polymorphism in paternity cases is discussed.", "contents": "Polymorphism of glyoxalase I in Vienna. Phenotype and gene frequencies of the GLO I polymorphism in Vienna are given. No exception to the postulated rule of inheritance could be found in 23 families with 51 children and 132 mother-child pairs. Linkage with the HLA system is confirmed, but no linkage disequilibrium between GLO alleles and HLA-A, B, C genes was detected. The use of the GLO I polymorphism in paternity cases is discussed."}
{"id": "PMID:478553", "title": "Polymorphism of red cell glutamic-pyruvic transaminase in Japanese: gene frequencies in samples from Northern Japan.", "content": "The genetic polymorphism of red cell GPT was investigated by starch-gel electrophoresis in four samples from northern Japan including a sample of the Ainu of Hokkaido. The distribution of the Gpt1 gene frequencies among 10 samples excluding the Ainu so far examined showed heterogeneity. The geographical cline of Gpt1 gene frequency reported in southern Japan was not observed in northern Japan.", "contents": "Polymorphism of red cell glutamic-pyruvic transaminase in Japanese: gene frequencies in samples from Northern Japan. The genetic polymorphism of red cell GPT was investigated by starch-gel electrophoresis in four samples from northern Japan including a sample of the Ainu of Hokkaido. The distribution of the Gpt1 gene frequencies among 10 samples excluding the Ainu so far examined showed heterogeneity. The geographical cline of Gpt1 gene frequency reported in southern Japan was not observed in northern Japan."}
{"id": "PMID:478554", "title": "Seven cases of branchial cyst and sinuses in four generations.", "content": "7 cases of isolated branchial anomalies distributed in four consecutive generations of a Central Indian family are described: 6 were males and 1 was female. 3 members had a left-sided branchial sinus, 1 had a right-sided sinus, 2 had bilateral sinuses, and 1 had a right-sided cyst. The family shows that branchial (lateral cervical) cysts and sinuses are inherited as autosomal dominant characters, and that the two anomalies are indistinguishable genetically.", "contents": "Seven cases of branchial cyst and sinuses in four generations. 7 cases of isolated branchial anomalies distributed in four consecutive generations of a Central Indian family are described: 6 were males and 1 was female. 3 members had a left-sided branchial sinus, 1 had a right-sided sinus, 2 had bilateral sinuses, and 1 had a right-sided cyst. The family shows that branchial (lateral cervical) cysts and sinuses are inherited as autosomal dominant characters, and that the two anomalies are indistinguishable genetically."}
{"id": "PMID:478555", "title": "Glyoxalase 1: a possible 'null' allele.", "content": "A three-generation family, ascertained through the presence of two diabetic sibs, provides segregation data suggestive of the existence of a null allele at the glyoxalase (GLO) locus. This conclusion is supported by the GLO 1 phenotype in two children from a GLO 2 father. These two children inherited the same paternal HLA allele, while two other sibs received GLO 2 with the other paternal HLA haplotype. The rest of the pedigree is in agreement with this suggestion, while the segregation of all other informative markers does not suggest nonpaternity.", "contents": "Glyoxalase 1: a possible 'null' allele. A three-generation family, ascertained through the presence of two diabetic sibs, provides segregation data suggestive of the existence of a null allele at the glyoxalase (GLO) locus. This conclusion is supported by the GLO 1 phenotype in two children from a GLO 2 father. These two children inherited the same paternal HLA allele, while two other sibs received GLO 2 with the other paternal HLA haplotype. The rest of the pedigree is in agreement with this suggestion, while the segregation of all other informative markers does not suggest nonpaternity."}
{"id": "PMID:478556", "title": "A study of the linkage relations of epidermolysis bullosa dystrophica.", "content": "Two large families from the Faroe Islands presenting epidermolysis bullosa of the dystrophic type were subjected to extensive linkage analyses with 22 serological markers. No significant evidence in support of linkage with any of these loci was provided. It was found to be very unlikely that the gene or genes causing the present types of epidermolysis bullosa belong to the EBS1 locus known to be closely linked to the GPT locus.", "contents": "A study of the linkage relations of epidermolysis bullosa dystrophica. Two large families from the Faroe Islands presenting epidermolysis bullosa of the dystrophic type were subjected to extensive linkage analyses with 22 serological markers. No significant evidence in support of linkage with any of these loci was provided. It was found to be very unlikely that the gene or genes causing the present types of epidermolysis bullosa belong to the EBS1 locus known to be closely linked to the GPT locus."}
{"id": "PMID:478557", "title": "Human alpha-L-fucosidase: a common polymorphic variant for low serum enzyme activity, studies of serum and leukocyte enzyme.", "content": "Serum alpha-L-fucosidase was assayed in 422 individuals. Extremely low levels of enzyme activity were found in 26 individuals. This variant enzyme has increased heat lability. The remaining samples exhibited a bimodal frequency distribution. Family studies suggest that the variant is inherited as a Mendelian recessive character. The variant leukocyte enzyme exhibits normal activity yet increased heat lability. Serum and leukocyte samples from individuals who do not possess the low activity variant are heterogeneous with respect to the heat lability of alpha-L-fucosidase. This common polymorphism may be useful for mapping purposes in family studies.", "contents": "Human alpha-L-fucosidase: a common polymorphic variant for low serum enzyme activity, studies of serum and leukocyte enzyme. Serum alpha-L-fucosidase was assayed in 422 individuals. Extremely low levels of enzyme activity were found in 26 individuals. This variant enzyme has increased heat lability. The remaining samples exhibited a bimodal frequency distribution. Family studies suggest that the variant is inherited as a Mendelian recessive character. The variant leukocyte enzyme exhibits normal activity yet increased heat lability. Serum and leukocyte samples from individuals who do not possess the low activity variant are heterogeneous with respect to the heat lability of alpha-L-fucosidase. This common polymorphism may be useful for mapping purposes in family studies."}
{"id": "PMID:478558", "title": "Genetic and nongenetic influences on the ABH and Lea antigen levels of saliva and milk.", "content": "The saliva and milk of 250 parturient women were studied in relation to ABH antigen levels; part of the sample was also investigated for the Lewis (Lea) substance. The levels of A and B are higher in saliva, and those of H and Lea higher in milk. The H average salivary titers presented the relationship O greater than A2 greater than A1 greater than B greater than AB, but these differences were not present in milk. In addition, the salivary levels of A and B are similar in individuals of these groups but B greater than A in AB persons, and A1 greater than A2; while in milk A greater than B in A, B and AB subjects, and A1 approximately equal to A2. The amount of Lea substance depends of the ABH secretor status in both secretions; but independently of this difference, the average titers were always higher in milk. Correlation coefficients between the levels observed in the two secretions are statistically significant for the A substance in A persons (0.46), H in B (0.58) and Lea in all subjects tested (0.47). A stepwise multiple regression analysis performed to verify the influence of four genetic and six nongenetic variables in the ABH levels of both fluids indicated only one consistent modifying factor: ABO type.", "contents": "Genetic and nongenetic influences on the ABH and Lea antigen levels of saliva and milk. The saliva and milk of 250 parturient women were studied in relation to ABH antigen levels; part of the sample was also investigated for the Lewis (Lea) substance. The levels of A and B are higher in saliva, and those of H and Lea higher in milk. The H average salivary titers presented the relationship O greater than A2 greater than A1 greater than B greater than AB, but these differences were not present in milk. In addition, the salivary levels of A and B are similar in individuals of these groups but B greater than A in AB persons, and A1 greater than A2; while in milk A greater than B in A, B and AB subjects, and A1 approximately equal to A2. The amount of Lea substance depends of the ABH secretor status in both secretions; but independently of this difference, the average titers were always higher in milk. Correlation coefficients between the levels observed in the two secretions are statistically significant for the A substance in A persons (0.46), H in B (0.58) and Lea in all subjects tested (0.47). A stepwise multiple regression analysis performed to verify the influence of four genetic and six nongenetic variables in the ABH levels of both fluids indicated only one consistent modifying factor: ABO type."}
{"id": "PMID:478559", "title": "Metabolism of mannose and fucose in cultured fibroblasts from patients with mannosidosis and mucolipidosis.", "content": "The incorporation of 14C-D-mannose or 14C-L-fucose into cultured fibroblasts from controls and patients with mannosidosis and mucolipidosis type II was studied. Mannose-containing oligosaccharide chains retarded on Sephadex G-25 were accumulated in cells from both patients. Fucose-containing oligosaccharides retarded on G-25 were also accumulated in cells from the patient with mucolipidosis. Besides there was an increase of macromolecular fucose-containing material in the cells from the patient with mucolipidosis. Conditioned medium of the cells from controls and the different patients exhibited about the same pattern as found in the cells, which might indicate leakage of the cellular material due to cell damage or death.", "contents": "Metabolism of mannose and fucose in cultured fibroblasts from patients with mannosidosis and mucolipidosis. The incorporation of 14C-D-mannose or 14C-L-fucose into cultured fibroblasts from controls and patients with mannosidosis and mucolipidosis type II was studied. Mannose-containing oligosaccharide chains retarded on Sephadex G-25 were accumulated in cells from both patients. Fucose-containing oligosaccharides retarded on G-25 were also accumulated in cells from the patient with mucolipidosis. Besides there was an increase of macromolecular fucose-containing material in the cells from the patient with mucolipidosis. Conditioned medium of the cells from controls and the different patients exhibited about the same pattern as found in the cells, which might indicate leakage of the cellular material due to cell damage or death."}
{"id": "PMID:478560", "title": "On the population genetics of beta2-glycoprotein I.", "content": "Beta2-glycoprotein I typings on 152 healthy Germans and 150 patients with atopic diseases did not show any differences in the serum protein concentrations or in the phenotype and gene frequencies. Compared to these German samples, Philippinos (n = 88) as well as healthy Negroes from South Africa (n = 192) revealed statistically significant lower concentrations of this serum protein. They differ also from the Germans with regard to phenotype and gene frequencies. A most striking result was found in the comparison of healthy and leprous Negroes (n = 250) from South Africa. In these, quite different and statistically significant beta 2-Glycoprotein I concentrations, respectively, phenotype and gene frequencies were seen, which may be due to this disease. The possible reasons for these observations as well as for the observed population differences are discussed.", "contents": "On the population genetics of beta2-glycoprotein I. Beta2-glycoprotein I typings on 152 healthy Germans and 150 patients with atopic diseases did not show any differences in the serum protein concentrations or in the phenotype and gene frequencies. Compared to these German samples, Philippinos (n = 88) as well as healthy Negroes from South Africa (n = 192) revealed statistically significant lower concentrations of this serum protein. They differ also from the Germans with regard to phenotype and gene frequencies. A most striking result was found in the comparison of healthy and leprous Negroes (n = 250) from South Africa. In these, quite different and statistically significant beta 2-Glycoprotein I concentrations, respectively, phenotype and gene frequencies were seen, which may be due to this disease. The possible reasons for these observations as well as for the observed population differences are discussed."}
{"id": "PMID:478561", "title": "A microtechnique for the rapid analysis of macromolecule synthesis in minicultures of mammalian cells.", "content": "A new micromethod, called the Stanzen technique, is described for the rapid determination of DNA and protein content as well as the incorporation rates of radioactively labeled precursors into macromolecules in cells growing in replica minicultures on plastic petri dishes. The procedure yielded reproducible results assaying only minimal cell numbers per sample and was applied for studying both primary or early passaged cell cultures (mouse epidermal cells and fibroblasts) and a malignantly transformed epidermal cell line. In four well defined circular areas (called Stanzen) marked on the bottom of tissue-culture plastic petri dishes (by heated stamps), 0.2 to 4 x 10(5) cells per area were plated and grown as four individual cultures in one dish. Both treatment and labeling with radioactive precursors of these Stanzen cultures were performed as with normal petri dishes. After fixation and extraction of the cultures, the singular Stanzen areas (with the cells fixed onto them) were sawed out and transferred into vials for liquid-scintillation counting or determination of DNA and protein. The obtained values of specific activity corresponded well whether the samples compared were derived from the minicultures of the same dish or from several dishes. By modifications of the known colorimetric methods for DNA and protein determination, the sensitivity of these procedures was improved down to values of 1 microgram DNA or 5 micrograms protein per individual culture. These micromodifications yielded the same values as the standard methods whether applied to cell suspensions or to cell cultures. Finally, cell proliferation was not influenced by the growth conditions in the small Stanzen areas and proceeded as in normal dishes or larger areas similarly stamped on the bottom of petri dishes. Since this method proved valuable for biochemical studies using primary cultures of mouse epidermal cells (saving cell material by a factor of 10, test substances and time), it might also be advantageous for other purposes as well where the availability of cells or test substances are limiting factors for large test series.", "contents": "A microtechnique for the rapid analysis of macromolecule synthesis in minicultures of mammalian cells. A new micromethod, called the Stanzen technique, is described for the rapid determination of DNA and protein content as well as the incorporation rates of radioactively labeled precursors into macromolecules in cells growing in replica minicultures on plastic petri dishes. The procedure yielded reproducible results assaying only minimal cell numbers per sample and was applied for studying both primary or early passaged cell cultures (mouse epidermal cells and fibroblasts) and a malignantly transformed epidermal cell line. In four well defined circular areas (called Stanzen) marked on the bottom of tissue-culture plastic petri dishes (by heated stamps), 0.2 to 4 x 10(5) cells per area were plated and grown as four individual cultures in one dish. Both treatment and labeling with radioactive precursors of these Stanzen cultures were performed as with normal petri dishes. After fixation and extraction of the cultures, the singular Stanzen areas (with the cells fixed onto them) were sawed out and transferred into vials for liquid-scintillation counting or determination of DNA and protein. The obtained values of specific activity corresponded well whether the samples compared were derived from the minicultures of the same dish or from several dishes. By modifications of the known colorimetric methods for DNA and protein determination, the sensitivity of these procedures was improved down to values of 1 microgram DNA or 5 micrograms protein per individual culture. These micromodifications yielded the same values as the standard methods whether applied to cell suspensions or to cell cultures. Finally, cell proliferation was not influenced by the growth conditions in the small Stanzen areas and proceeded as in normal dishes or larger areas similarly stamped on the bottom of petri dishes. Since this method proved valuable for biochemical studies using primary cultures of mouse epidermal cells (saving cell material by a factor of 10, test substances and time), it might also be advantageous for other purposes as well where the availability of cells or test substances are limiting factors for large test series."}
{"id": "PMID:478562", "title": "Mutagenic effect on L5178Y mouse lymphoma cells by growth in ethylene oxide-sterilized polycarbonate flasks.", "content": "The mutation frequency of L5178Y mouse lymphoma cells to resistance to 5'-bromo-2'-deoxyuridine increased 6- to 14-fold after growth in ethylene oxide-sterilized polycarbonate culture flasks compared to growth in glass flasks. No comparable increase was observed when L5178Y cells were grown in identical polycarbonate culture flasks sterilized by autoclaving.", "contents": "Mutagenic effect on L5178Y mouse lymphoma cells by growth in ethylene oxide-sterilized polycarbonate flasks. The mutation frequency of L5178Y mouse lymphoma cells to resistance to 5'-bromo-2'-deoxyuridine increased 6- to 14-fold after growth in ethylene oxide-sterilized polycarbonate culture flasks compared to growth in glass flasks. No comparable increase was observed when L5178Y cells were grown in identical polycarbonate culture flasks sterilized by autoclaving."}
{"id": "PMID:478563", "title": "Biological properties of human melanoma cells in culture.", "content": "Three human melanoma cell lines derived from one primary and two metastatic tumors from three different patients were characterized for growth properties usually associated with malignant transformation; these include cell morphology, growth rate, saturation density, growth in semisolid media, colony-forming ability on contact-inhibited monolayers of normal fibroblasts and epithelial cells, and tumorigenicity in immunosuppressed mice. Variations in expression of aberrant properties were evident among the lines. One of the metastatic lines satisfied all the parameters of malignancy tested and the other showed a number of these properties, whereas the primary essentially fulfilled only one. These results suggest that cultured melanoma cells reflect the clinical variability often observed among melanoma patients and that metastatic melanoma seems to display a higher degree of malignant transformation than the primary.", "contents": "Biological properties of human melanoma cells in culture. Three human melanoma cell lines derived from one primary and two metastatic tumors from three different patients were characterized for growth properties usually associated with malignant transformation; these include cell morphology, growth rate, saturation density, growth in semisolid media, colony-forming ability on contact-inhibited monolayers of normal fibroblasts and epithelial cells, and tumorigenicity in immunosuppressed mice. Variations in expression of aberrant properties were evident among the lines. One of the metastatic lines satisfied all the parameters of malignancy tested and the other showed a number of these properties, whereas the primary essentially fulfilled only one. These results suggest that cultured melanoma cells reflect the clinical variability often observed among melanoma patients and that metastatic melanoma seems to display a higher degree of malignant transformation than the primary."}
{"id": "PMID:478565", "title": "Normal human endometrium in cell culture. II. A microspectrophotometric study of polyploid nuclei in short-term primary epithelial cultures.", "content": "The growth of short-term primary cultures of endometrial epithelium has been studied using Feulgen microspectrophotometry. A gradual increase in the number of polyploid nuclei up to 64C has been observed and is associated with a decline in the growth capacity of the cultures. The specific mechanism(s) of this polyploidization is not known.", "contents": "Normal human endometrium in cell culture. II. A microspectrophotometric study of polyploid nuclei in short-term primary epithelial cultures. The growth of short-term primary cultures of endometrial epithelium has been studied using Feulgen microspectrophotometry. A gradual increase in the number of polyploid nuclei up to 64C has been observed and is associated with a decline in the growth capacity of the cultures. The specific mechanism(s) of this polyploidization is not known."}
{"id": "PMID:478564", "title": "The use of agarose in the determination of anchorage-independent growth.", "content": "At the present time, growth in agar suspension is one of the best in vitro correlates of tumorigenicity. Growth in agarose, however, has not been evaluated extensively as an in vitro criterion for tumorigenicity. In the present study we have tested 19 cell lines, including six mouse-human hybrids, for growth in agarose and agar in the presence and absence of exogenous hypoxanthine. None of the six nontumorigenic cell lines grew in agar or agarose. Ten of the 13 tumorigenic cell lines grew in both agar and agarose with about equal efficiency. The remaining three tumorigenic cell lines grew well in agarose but poorly or not at all in agar. Hypoxanthine did not stimulate the growth in agar or agarose of any of the cell lines except BHK. We conclude that growth in agarose may be a more sensitive marker for tumorigenicity than growth in agar and that BHK is exceptional in its sensitivity to supplemental purines.", "contents": "The use of agarose in the determination of anchorage-independent growth. At the present time, growth in agar suspension is one of the best in vitro correlates of tumorigenicity. Growth in agarose, however, has not been evaluated extensively as an in vitro criterion for tumorigenicity. In the present study we have tested 19 cell lines, including six mouse-human hybrids, for growth in agarose and agar in the presence and absence of exogenous hypoxanthine. None of the six nontumorigenic cell lines grew in agar or agarose. Ten of the 13 tumorigenic cell lines grew in both agar and agarose with about equal efficiency. The remaining three tumorigenic cell lines grew well in agarose but poorly or not at all in agar. Hypoxanthine did not stimulate the growth in agar or agarose of any of the cell lines except BHK. We conclude that growth in agarose may be a more sensitive marker for tumorigenicity than growth in agar and that BHK is exceptional in its sensitivity to supplemental purines."}
{"id": "PMID:478566", "title": "Selection of a chemically defined medium for culturing adult newt forelimb regenerates.", "content": "Three commercially available tissue-culture media were evaluated for their ability to support continued growth and differentiation of 14-day regenerates of adult newt forelimbs. Serums, embryo extracts, egg ultrafiltrates, and antimicrobial agents were avoided in this analysis. The hormones insulin and L-thyroxine were added to these chemically defined media to enhance continued cellular metabolism and growth. The optimum conditions appeared to be cultivated at 25 degrees C (pH 7.2 to 7.4) in media osmotically adjusted to conditions approximating amphibian blood values (i.e. 225 m0SM for 199, 244 m0SM for CMRL-1066, and 262 m0SM FOR L-15).", "contents": "Selection of a chemically defined medium for culturing adult newt forelimb regenerates. Three commercially available tissue-culture media were evaluated for their ability to support continued growth and differentiation of 14-day regenerates of adult newt forelimbs. Serums, embryo extracts, egg ultrafiltrates, and antimicrobial agents were avoided in this analysis. The hormones insulin and L-thyroxine were added to these chemically defined media to enhance continued cellular metabolism and growth. The optimum conditions appeared to be cultivated at 25 degrees C (pH 7.2 to 7.4) in media osmotically adjusted to conditions approximating amphibian blood values (i.e. 225 m0SM for 199, 244 m0SM for CMRL-1066, and 262 m0SM FOR L-15)."}
{"id": "PMID:478567", "title": "Polarographic measurement of oxygen uptake by astrocytes in primary cultures using the tissue-culture flask as the respirometer chamber.", "content": "Oxygen uptake was measured in primary cultures of astrocytes from the brain hemispheres of newborn DBA mice by the aid of an oxygen electrode inserted directly into the culture flask, i.e. using the flask, completely filled with MEM medium, as the respirometer chamber. The respiration was initially intense (300 mumol per hr per 100 mg protein) but declined somewhat during the 6 hr of measurement, probably due to a depletion of intermediary metabolites released to the large surplus of medium. The respiratory rates were approximately identical in the presence of a CO2/bicarbonate and a HEPES buffer. Exposure to a high concentration of potassium led to a transient stimulation of the oxygen uptake to almost 100%, a response that was very easily observed using the present method. Since no mechanical damage was inflicted upon the cells, culturing could be continued, if so desired, after the measurement.", "contents": "Polarographic measurement of oxygen uptake by astrocytes in primary cultures using the tissue-culture flask as the respirometer chamber. Oxygen uptake was measured in primary cultures of astrocytes from the brain hemispheres of newborn DBA mice by the aid of an oxygen electrode inserted directly into the culture flask, i.e. using the flask, completely filled with MEM medium, as the respirometer chamber. The respiration was initially intense (300 mumol per hr per 100 mg protein) but declined somewhat during the 6 hr of measurement, probably due to a depletion of intermediary metabolites released to the large surplus of medium. The respiratory rates were approximately identical in the presence of a CO2/bicarbonate and a HEPES buffer. Exposure to a high concentration of potassium led to a transient stimulation of the oxygen uptake to almost 100%, a response that was very easily observed using the present method. Since no mechanical damage was inflicted upon the cells, culturing could be continued, if so desired, after the measurement."}
{"id": "PMID:478568", "title": "Sensitivity of cultured rat hepatocytes to aflatoxin B1 and benzo(a)pyrene.", "content": "We have tested the sensitivity of a cloned rat hepatocyte line, RL-PR-C, to aflatoxin B1 and benzo(a)pyrene as a function of population-doubling level. The cells were much more sensitive to the cytotoxic action of these agents subsequent to 230 population doublings. This sensitivity corresponded to the enhanced inducibility of aryl hydrocarbon hydroxylase activity by 3-methylcholanthrene.", "contents": "Sensitivity of cultured rat hepatocytes to aflatoxin B1 and benzo(a)pyrene. We have tested the sensitivity of a cloned rat hepatocyte line, RL-PR-C, to aflatoxin B1 and benzo(a)pyrene as a function of population-doubling level. The cells were much more sensitive to the cytotoxic action of these agents subsequent to 230 population doublings. This sensitivity corresponded to the enhanced inducibility of aryl hydrocarbon hydroxylase activity by 3-methylcholanthrene."}
{"id": "PMID:478579", "title": "[Determination of the bactericidal activity of peritoneal macrophages: a new method (author's transl)].", "content": "A new method to estimate the bactericidal activity of peritoneal macrophages is described. This method allows to calculate with great accuracy the number of phagocytised bacteria per macrophage. In serial experiments different degrees of activation of macrophages in respect to their bactericidal activity against listeria monocytogenes has been shown.", "contents": "[Determination of the bactericidal activity of peritoneal macrophages: a new method (author's transl)]. A new method to estimate the bactericidal activity of peritoneal macrophages is described. This method allows to calculate with great accuracy the number of phagocytised bacteria per macrophage. In serial experiments different degrees of activation of macrophages in respect to their bactericidal activity against listeria monocytogenes has been shown."}
{"id": "PMID:478580", "title": "[Studies on normal range of serum IgE concentration (author's transl)].", "content": "To obtain information about the normal range of IgE concentration in serum a random population of male and female patients was divided into 14 age groups. Based on the mean values (x +/- 1s) an age dependency is clearly recognizable with an increase of the IgE concentration at one and two years of age, a constant IgE concentration during the middle age period, and a decrease of the IgE level in the 40th year of age which becomes especially obvious after the 60th and 80th year of age. From the distribution pattern of IgE levels within the different age groups it is evident that only the curves of newborns and persons over 80 years resemble a normal Gaussian distribution. The other age groups show 2 or 3 peaks of IgE. However, these peaks represent only 50% of the total IgE concentration. A similar distribution pattern was obtained by IgE determinations in 100 \"healthy\" blood donors which showed no clinical evidence of allergy. The age dependent normal ranges given in this paper make it possible to evaluate increased IgE levels accordingly.", "contents": "[Studies on normal range of serum IgE concentration (author's transl)]. To obtain information about the normal range of IgE concentration in serum a random population of male and female patients was divided into 14 age groups. Based on the mean values (x +/- 1s) an age dependency is clearly recognizable with an increase of the IgE concentration at one and two years of age, a constant IgE concentration during the middle age period, and a decrease of the IgE level in the 40th year of age which becomes especially obvious after the 60th and 80th year of age. From the distribution pattern of IgE levels within the different age groups it is evident that only the curves of newborns and persons over 80 years resemble a normal Gaussian distribution. The other age groups show 2 or 3 peaks of IgE. However, these peaks represent only 50% of the total IgE concentration. A similar distribution pattern was obtained by IgE determinations in 100 \"healthy\" blood donors which showed no clinical evidence of allergy. The age dependent normal ranges given in this paper make it possible to evaluate increased IgE levels accordingly."}
{"id": "PMID:478592", "title": "Chemotactic factor inactivator release from rat leukocytes.", "content": "Rat neutrophils, alveolar macrophages, and peritoneal macrophages each released chemotactic factor inactivator (CFI) activities under conditions of endocytosis (uptake of opsonized zymosan particles and preformed immune complexes). CFI activities in supernatant fluids from phagocytizing leukocytes were found for the chemotactic factors from the third (C3) and the fifth (C5) components of complement and for the bacterial chemotactic factor (present in culture supernatant fluids from Escherichia coli.) CFI activity could also be demonstrated in homogenates obtained from disrupted leukocytes. CFI release from intact leukocytes was dependent on the duration of incubation of leukocytes with the phagocytic stimulus. No quantitative relationships were noted between the amount of CFI activity and the amount of beta-glucuronidase in phagocytic supernatant fluids released from leukocytes. The release of CFI activities from phagocytizing leukocytes may represent a regulatory (\"turn-off\") mechanism for the inflammatory response.", "contents": "Chemotactic factor inactivator release from rat leukocytes. Rat neutrophils, alveolar macrophages, and peritoneal macrophages each released chemotactic factor inactivator (CFI) activities under conditions of endocytosis (uptake of opsonized zymosan particles and preformed immune complexes). CFI activities in supernatant fluids from phagocytizing leukocytes were found for the chemotactic factors from the third (C3) and the fifth (C5) components of complement and for the bacterial chemotactic factor (present in culture supernatant fluids from Escherichia coli.) CFI activity could also be demonstrated in homogenates obtained from disrupted leukocytes. CFI release from intact leukocytes was dependent on the duration of incubation of leukocytes with the phagocytic stimulus. No quantitative relationships were noted between the amount of CFI activity and the amount of beta-glucuronidase in phagocytic supernatant fluids released from leukocytes. The release of CFI activities from phagocytizing leukocytes may represent a regulatory (\"turn-off\") mechanism for the inflammatory response."}
{"id": "PMID:478593", "title": "An assay procedure for the comparative irritancy testing of esters in the tigliane and daphnane series.", "content": "A method is described for testing of diterpene esters for irritancy. The technique involves the application of acetone solutions of the toxins to the inside ears of female LACA mice. The number of mice responding per group and the log10 dose data were evaluated using probit analysis with the assistance of a computer program. This evaluation has the advantage that approximations inherent with an arithmetical evaluation were eliminated, and limits may be placed upon the standard deviation of the irritant dose 50% (ID50). In addition, the use of a chi2 test automatically eliminated results which were not attributed to random biological variation. Observations of the time to onset and the persistence of the inflammation have led to the suggestion that daphnane orthoester diterpenes may elicit their effect by means of a direct action at a receptor site in skin, whereas the tigliane O-acyl esters may in part act by causing more general tissue damage.", "contents": "An assay procedure for the comparative irritancy testing of esters in the tigliane and daphnane series. A method is described for testing of diterpene esters for irritancy. The technique involves the application of acetone solutions of the toxins to the inside ears of female LACA mice. The number of mice responding per group and the log10 dose data were evaluated using probit analysis with the assistance of a computer program. This evaluation has the advantage that approximations inherent with an arithmetical evaluation were eliminated, and limits may be placed upon the standard deviation of the irritant dose 50% (ID50). In addition, the use of a chi2 test automatically eliminated results which were not attributed to random biological variation. Observations of the time to onset and the persistence of the inflammation have led to the suggestion that daphnane orthoester diterpenes may elicit their effect by means of a direct action at a receptor site in skin, whereas the tigliane O-acyl esters may in part act by causing more general tissue damage."}
{"id": "PMID:478594", "title": "The effect of selected amino acids on gelatin-induced inflammation in adult male mice.", "content": "Several studies have indicated to us that certain amino acids may exhibit antiinflammatory activity. In the present study we attempted to evaluate the inhibitory effect of various amino acids on gelatin-induced abdominal inflammation in mice using peritoneal fluid cytology as the diagnostic tool. The L-amino acids tested were tryptophan, phenylalanine, alanine, cystine, hydroxyproline, tyrosine, citrulline, leucine, and valine. Hydrocortisone was used as an antiphlogistic steroid control. Tryptophan, phenylalanine, alanine, cystine, hydroxyproline, and tyrosine all significantly decreased the inflammation. Citrulline and valine, however, both exhibited strong antiiflammatory responses. Based on these results, three related dipeptides were also screened: L-valy-L-alanine, L-valyl-L-tryptophan, and L-tyrosl-L-valine. Valyl alanine was found to produce a strong antiinflammatory effect. In a final test, the combination of the steroid, hydrocortisone, and the amino acid, cystine, was screened for a synergistic effect. The combined treatment inhibited the gelatin-induced inflammation more than either the amino acid or the steroid administered alone.", "contents": "The effect of selected amino acids on gelatin-induced inflammation in adult male mice. Several studies have indicated to us that certain amino acids may exhibit antiinflammatory activity. In the present study we attempted to evaluate the inhibitory effect of various amino acids on gelatin-induced abdominal inflammation in mice using peritoneal fluid cytology as the diagnostic tool. The L-amino acids tested were tryptophan, phenylalanine, alanine, cystine, hydroxyproline, tyrosine, citrulline, leucine, and valine. Hydrocortisone was used as an antiphlogistic steroid control. Tryptophan, phenylalanine, alanine, cystine, hydroxyproline, and tyrosine all significantly decreased the inflammation. Citrulline and valine, however, both exhibited strong antiiflammatory responses. Based on these results, three related dipeptides were also screened: L-valy-L-alanine, L-valyl-L-tryptophan, and L-tyrosl-L-valine. Valyl alanine was found to produce a strong antiinflammatory effect. In a final test, the combination of the steroid, hydrocortisone, and the amino acid, cystine, was screened for a synergistic effect. The combined treatment inhibited the gelatin-induced inflammation more than either the amino acid or the steroid administered alone."}
{"id": "PMID:478595", "title": "Acute induction of joint inflammation in the rat by poly I . poly C.", "content": "Intraarticular injection of interferon inducer, double-stranded polyinosinate-polycytidylate (Poly I . C) caused acute synovitis in rats. This acute inflammatory response was accompanied by an increased concentration of prostaglandin E (PGE) in the synovial tissue. Double-stranded polyadenylate-polyuridylate (Poly A . Poly U) was less potent than Poly I . Poly C in inducing synovitis and increasing PGE concentration, while single-stranded polyinosinate (Poly I) or polycytidylate (Poly C) were inactive in these respects. Intraarticular injection of partially purified mouse fibroblast interferon also induced synovial inflammation. The present results suggest that interferon may be a mediator of viral inflammatory responses.", "contents": "Acute induction of joint inflammation in the rat by poly I . poly C. Intraarticular injection of interferon inducer, double-stranded polyinosinate-polycytidylate (Poly I . C) caused acute synovitis in rats. This acute inflammatory response was accompanied by an increased concentration of prostaglandin E (PGE) in the synovial tissue. Double-stranded polyadenylate-polyuridylate (Poly A . Poly U) was less potent than Poly I . Poly C in inducing synovitis and increasing PGE concentration, while single-stranded polyinosinate (Poly I) or polycytidylate (Poly C) were inactive in these respects. Intraarticular injection of partially purified mouse fibroblast interferon also induced synovial inflammation. The present results suggest that interferon may be a mediator of viral inflammatory responses."}
{"id": "PMID:478596", "title": "Pulmonary macrophage growth factor.", "content": "Pulmonary macrophage growth factor (MCF) can be purified from the \"used\" serum-free medium of cultures of mouse lung cells by concentration and dialysis over a 30,000-dalton Amicon ultrafilter and subjecting the retentate to isoelectric focusing. This yields a protein fraction with an isolelectric point of pH 4.2, which contains all the MGF activity. Upon electrophoresis of this fraction in analytical gels, one large and four small bands could be visualized. All bands were biologically active. This same \"heterogenous\" fraction ran as one band (mol wt 68,000) in SDS analytical gel electrophoresis, suggesting the ordered aggregation of this monomer. This was confirmed by reelectrophoresis of the first (and largest) band demonstrating the same 5-banded pattern as the initial material.", "contents": "Pulmonary macrophage growth factor. Pulmonary macrophage growth factor (MCF) can be purified from the \"used\" serum-free medium of cultures of mouse lung cells by concentration and dialysis over a 30,000-dalton Amicon ultrafilter and subjecting the retentate to isoelectric focusing. This yields a protein fraction with an isolelectric point of pH 4.2, which contains all the MGF activity. Upon electrophoresis of this fraction in analytical gels, one large and four small bands could be visualized. All bands were biologically active. This same \"heterogenous\" fraction ran as one band (mol wt 68,000) in SDS analytical gel electrophoresis, suggesting the ordered aggregation of this monomer. This was confirmed by reelectrophoresis of the first (and largest) band demonstrating the same 5-banded pattern as the initial material."}
{"id": "PMID:478597", "title": "Investigations into the skin-irritant properties of resiniferonol ortho esters.", "content": "A series of esters were produced by partial synthesis from 9,13,14-orthophenylacetyl-resiniferonol. These compounds were tested for irritant effects by means of a mouse ear assay. All of the derivatives, including the parent compound, produced short-term inflammation of mice ears within 1-2 h and the effects did not persist for 24 h. This is in contrast to esters of structurally related tigliane diterpenes which produce a longer-term on mice ears. Highly potent irritants were synthesized which exhibited irritant doses 50% in the 0.0012-0.00021 nmol range. These esters were all substituted phenylacetates of the C-20 position of 9,13,14-orthophenylacethyl-resiniferonol. The meta or para positions of the phenylacetate moiety were substituted with electronegative groups for maximum activity. Compounds exhibiting substituted phenyl propionates at C20 were not irritants in the test used.", "contents": "Investigations into the skin-irritant properties of resiniferonol ortho esters. A series of esters were produced by partial synthesis from 9,13,14-orthophenylacetyl-resiniferonol. These compounds were tested for irritant effects by means of a mouse ear assay. All of the derivatives, including the parent compound, produced short-term inflammation of mice ears within 1-2 h and the effects did not persist for 24 h. This is in contrast to esters of structurally related tigliane diterpenes which produce a longer-term on mice ears. Highly potent irritants were synthesized which exhibited irritant doses 50% in the 0.0012-0.00021 nmol range. These esters were all substituted phenylacetates of the C-20 position of 9,13,14-orthophenylacethyl-resiniferonol. The meta or para positions of the phenylacetate moiety were substituted with electronegative groups for maximum activity. Compounds exhibiting substituted phenyl propionates at C20 were not irritants in the test used."}
{"id": "PMID:478598", "title": "Effect of inflammatory and noninflammatory stress on plasma ketone bodies and free fatty acids and on glucagon and insulin in peripheral and portal blood.", "content": "Inflammatory stress as characterized by infection with Streptococcus pneumoniae, administration of endotoxin, or the induction of a turpentine abscess is characterized by the inhibition of the ketosis assoicated with fasting and a decline in the level of free fatty acids in the plasma. Moreover, rats subjectd to these inflammatory stresses demonstrate a significant rise in peripheral and portal insulin and glucagon. Rats subjected to noninflammatory stresses, screen-restraint, or noninvasive femoral fracture did not demonstrate the inhibition of ketosis but did show a decrease in plasma free fatty acids. The noninflammatroy stresses did not show an abnormal elevation of plasma or portal insulin or glucagon.", "contents": "Effect of inflammatory and noninflammatory stress on plasma ketone bodies and free fatty acids and on glucagon and insulin in peripheral and portal blood. Inflammatory stress as characterized by infection with Streptococcus pneumoniae, administration of endotoxin, or the induction of a turpentine abscess is characterized by the inhibition of the ketosis assoicated with fasting and a decline in the level of free fatty acids in the plasma. Moreover, rats subjectd to these inflammatory stresses demonstrate a significant rise in peripheral and portal insulin and glucagon. Rats subjected to noninflammatory stresses, screen-restraint, or noninvasive femoral fracture did not demonstrate the inhibition of ketosis but did show a decrease in plasma free fatty acids. The noninflammatroy stresses did not show an abnormal elevation of plasma or portal insulin or glucagon."}
{"id": "PMID:478599", "title": "Leukocyte migration inhibition activity of nonimmune acute inflammatory pleural exudate.", "content": "Material with leuckocyte migration inhibition (LMI) activity has been demonstrated in various types of nonimmunologically induced acute pleural inflammatory exudates. This activity is present in inflammatory cell-free exudate and appears to involved the deposition of fibrin around the migrating leukocyte, resulting in \"cell-trapping.\" This is supported by the fact that removal or inhibition of fibrin formation leads to loss of exudate LMI activity. Both fibrinogen and complement as well as vitamin K-dependent clotting factors appear to be required for LMI activity. The mechanism involved in the LMI reaction and its significance in nonimmune and cell-mediated immune inflammation are discussed.", "contents": "Leukocyte migration inhibition activity of nonimmune acute inflammatory pleural exudate. Material with leuckocyte migration inhibition (LMI) activity has been demonstrated in various types of nonimmunologically induced acute pleural inflammatory exudates. This activity is present in inflammatory cell-free exudate and appears to involved the deposition of fibrin around the migrating leukocyte, resulting in \"cell-trapping.\" This is supported by the fact that removal or inhibition of fibrin formation leads to loss of exudate LMI activity. Both fibrinogen and complement as well as vitamin K-dependent clotting factors appear to be required for LMI activity. The mechanism involved in the LMI reaction and its significance in nonimmune and cell-mediated immune inflammation are discussed."}
{"id": "PMID:478600", "title": "Neutrophil enzyme activities in rheumatoid inflammation.", "content": "This study analyzed the neutrophils and sera of patients with rheumatoid arthritis and of normal controls. No significant differences were found in the activities of the granular enzymes beta-glucuronidase and lysozyme or the cytoplasmic enzyme lactic dehydrogenase (LDH). Normal neutrophils were found to release significant (P less than 0.05) amounts of the granular enzymes, but not of LDH in response to immunoglobulin G aggregates. There was no difference in the percent release exhibited by rheumatoid versus control neutrophils. Studies delineating the effects of rheumatoid factor sera and normal sera on aggregate-induced enzyme release revealed a significant negative correlation between the amount of rheumatoid factor in the sera and the percent release of beta-glucuronidase and lysozyme but not of LDH. These studies thus demonstrate no abnormalities in rheumatoid neutrophil or rheumatoid serum enzyme activities or in neutrophil response to immunoglobulin G aggregates. They suggest, however, that rheumatoid factor may partially inhibit the release of lysosomal enzymes, thus suppressing this important component of the rheumatoid inflammatory process.", "contents": "Neutrophil enzyme activities in rheumatoid inflammation. This study analyzed the neutrophils and sera of patients with rheumatoid arthritis and of normal controls. No significant differences were found in the activities of the granular enzymes beta-glucuronidase and lysozyme or the cytoplasmic enzyme lactic dehydrogenase (LDH). Normal neutrophils were found to release significant (P less than 0.05) amounts of the granular enzymes, but not of LDH in response to immunoglobulin G aggregates. There was no difference in the percent release exhibited by rheumatoid versus control neutrophils. Studies delineating the effects of rheumatoid factor sera and normal sera on aggregate-induced enzyme release revealed a significant negative correlation between the amount of rheumatoid factor in the sera and the percent release of beta-glucuronidase and lysozyme but not of LDH. These studies thus demonstrate no abnormalities in rheumatoid neutrophil or rheumatoid serum enzyme activities or in neutrophil response to immunoglobulin G aggregates. They suggest, however, that rheumatoid factor may partially inhibit the release of lysosomal enzymes, thus suppressing this important component of the rheumatoid inflammatory process."}
{"id": "PMID:478620", "title": "Bone healing in rabbits after compression osteosynthesis: a comparative study between the radiological and histological findings.", "content": "The relationship between the radiological and histological findings was studied in a series of rabbit osteotomies and fractures stabilized with plates with and without longitudinal compression. When compression was employed, the gap varied between 10 and 200 micrometers and no radiologically visible callus was seen. After 4 weeks the smallest gaps of 10--30 micrometers were filled histologically with structureless material, while the larger gaps contained 'cortical' bone derived from periosteal and endosteal proliferation. The degree of union could not be assessed from X-ray examination, as there was no correlation between the histological and radiological appearances. When no compression was employed, the gap varied between 200--1200 micrometers; radiologically visible periosteal callus and filling of the gap indicated that consolidation had appeared in four weeks. Gaps of 200--500 micrometers were filled with bone derived from the periosteum and endosteum of lamellar structure which had invaded the original cortical bone ('plugging'). When the gaps were more than 500 micrometers, islands of cartilage and connective tissue were found. In all cases studied, many 'anchoring osteons' passed from the endosteal and periosteal callus into the original cortical bone. It is concluded that a 'compression internal fixation' of cortical bone will not necessarily secure union, as histologically confirmed, even when the formation of radiologically visible callus is suppressed. The lack of periosteal callus appears to be the result of a small gap and rigid fixation, rather than related to strains induced in the bone.", "contents": "Bone healing in rabbits after compression osteosynthesis: a comparative study between the radiological and histological findings. The relationship between the radiological and histological findings was studied in a series of rabbit osteotomies and fractures stabilized with plates with and without longitudinal compression. When compression was employed, the gap varied between 10 and 200 micrometers and no radiologically visible callus was seen. After 4 weeks the smallest gaps of 10--30 micrometers were filled histologically with structureless material, while the larger gaps contained 'cortical' bone derived from periosteal and endosteal proliferation. The degree of union could not be assessed from X-ray examination, as there was no correlation between the histological and radiological appearances. When no compression was employed, the gap varied between 200--1200 micrometers; radiologically visible periosteal callus and filling of the gap indicated that consolidation had appeared in four weeks. Gaps of 200--500 micrometers were filled with bone derived from the periosteum and endosteum of lamellar structure which had invaded the original cortical bone ('plugging'). When the gaps were more than 500 micrometers, islands of cartilage and connective tissue were found. In all cases studied, many 'anchoring osteons' passed from the endosteal and periosteal callus into the original cortical bone. It is concluded that a 'compression internal fixation' of cortical bone will not necessarily secure union, as histologically confirmed, even when the formation of radiologically visible callus is suppressed. The lack of periosteal callus appears to be the result of a small gap and rigid fixation, rather than related to strains induced in the bone."}
{"id": "PMID:478621", "title": "Internal fixation of fractures of the proximal end of the radius in adults.", "content": "Eight cases of internal fixation for fractures of the proximal end of the radius in adults are discussed. The reasons for this procedure, after considering the patient's age and activity and the type of fracture, was to avoid the sequelae of resection of the radial head. By following an appropriate technique, and provided that there are no compications, the results obtained have been excellent.", "contents": "Internal fixation of fractures of the proximal end of the radius in adults. Eight cases of internal fixation for fractures of the proximal end of the radius in adults are discussed. The reasons for this procedure, after considering the patient's age and activity and the type of fracture, was to avoid the sequelae of resection of the radial head. By following an appropriate technique, and provided that there are no compications, the results obtained have been excellent."}
{"id": "PMID:478622", "title": "A universal snap-fit endoprosthesis for the treatment of subcapital fractures of the femur: a preliminary report.", "content": "A new endoprosthesis for the treatment of subcapital fractures is described. It is hemispherical with a high density polyethylene (HDP) core which is snap-fitted onto a Charnley-type femoral prosthesis. The aim is to prevent acetabular erosion by its virtual lack of movement of the cartilage-metal interface. If this should occur then conversion to a total hip is readily performed without removing the femoral stem. So far 14 have been inserted without any complications. In fact, they are easily mobilized with very little pain.", "contents": "A universal snap-fit endoprosthesis for the treatment of subcapital fractures of the femur: a preliminary report. A new endoprosthesis for the treatment of subcapital fractures is described. It is hemispherical with a high density polyethylene (HDP) core which is snap-fitted onto a Charnley-type femoral prosthesis. The aim is to prevent acetabular erosion by its virtual lack of movement of the cartilage-metal interface. If this should occur then conversion to a total hip is readily performed without removing the femoral stem. So far 14 have been inserted without any complications. In fact, they are easily mobilized with very little pain."}
{"id": "PMID:478623", "title": "Internal fixation of fractures of the neck of the femur in hemiplegic patients.", "content": "There was a history of hemiplegia due to stroke in 97 (6.5 per cent) of 1483 consecutive patients with fresh fractures of the neck of the femur. Both cervical and trochanteric features occurred significantly more often on the hemiplegic side. Internal fixation was technically satisfactory in trochanteric fractures, but failed frequently in cervical fractures.", "contents": "Internal fixation of fractures of the neck of the femur in hemiplegic patients. There was a history of hemiplegia due to stroke in 97 (6.5 per cent) of 1483 consecutive patients with fresh fractures of the neck of the femur. Both cervical and trochanteric features occurred significantly more often on the hemiplegic side. Internal fixation was technically satisfactory in trochanteric fractures, but failed frequently in cervical fractures."}
{"id": "PMID:478625", "title": "Varus deformity in supracondylar fractures of the humerus in children.", "content": "Eighty cases of supracondylar fracture of the humerus at the elbow in children have been analysed. The post-reduction radiograph of the fracture was classified depending on the residual deformity present and related to the loss of carrying angle and cubitus varus deformity at the time of follow-up. Fifty-four percent of the displaced fractures showed a loss of carrying angle greater than 5 degrees, and of these 23 per cent developed cubitus varus deformity. Eighty-five per cent of those cases with a loss of carrying angle greater than 10 degrees showed either residual tilt or medial rotation on the post-reduction radiograph. All cases in which there was residual medial tilt after manipulation and 40 per cent of those with medial rotation on the post-reduction radiograph developed cubitus varus. In our series, there was no evidence of epiphyseal injury causing the deformity and in none of the cases was the deformity progressive. It is suggested that, in order to reduce the incidence of varus deformity in suprocondylar fractures, open reduction and internal fixation is indicated in those fractures in which tilt or medial rotation cannot be reduced by conservative means.", "contents": "Varus deformity in supracondylar fractures of the humerus in children. Eighty cases of supracondylar fracture of the humerus at the elbow in children have been analysed. The post-reduction radiograph of the fracture was classified depending on the residual deformity present and related to the loss of carrying angle and cubitus varus deformity at the time of follow-up. Fifty-four percent of the displaced fractures showed a loss of carrying angle greater than 5 degrees, and of these 23 per cent developed cubitus varus deformity. Eighty-five per cent of those cases with a loss of carrying angle greater than 10 degrees showed either residual tilt or medial rotation on the post-reduction radiograph. All cases in which there was residual medial tilt after manipulation and 40 per cent of those with medial rotation on the post-reduction radiograph developed cubitus varus. In our series, there was no evidence of epiphyseal injury causing the deformity and in none of the cases was the deformity progressive. It is suggested that, in order to reduce the incidence of varus deformity in suprocondylar fractures, open reduction and internal fixation is indicated in those fractures in which tilt or medial rotation cannot be reduced by conservative means."}
{"id": "PMID:478626", "title": "The unstable shoulder: recurring subluxation.", "content": "A review of operative repairs for recurring dislocation of the shoulder revealed that a significant proportion of these operations was being done for what could best be described as recurring subluxation of the shoulder. The presenting complaint was of the shoulder 'going out of joint', but no significant trauma was recalled, dislocation was never shown on the radiograph and none required manual reduction. The only physical finding was apprehension on external rotation of the shoulder in abduction. Radiographs were frequently normal and arthrography and cineradiography were not helpful in confirming anterior displacement. The most useful preoperative information was obtained by manipulation of the shoulder under general anaesthesia just before the surgical repair. In all instances anterior instability could be demonstrated. Of 99 Magnuson-Stack repairs drawn from the records of the Vancouver General Hospital in a 3-year period, 34 proved to be examples of recurring subluxation. This high proportion of such patients contradicts the teaching in standard orthopaedic textbooks, but substantiates the warning of Rowe (1963) to beware of the patient whose shoulder 'dislocates' initially with little evidence of injury. It also substantiates Saha's concept (1971) of inherent shoulder instability as a contributor to the incidence of recurring dislocation.", "contents": "The unstable shoulder: recurring subluxation. A review of operative repairs for recurring dislocation of the shoulder revealed that a significant proportion of these operations was being done for what could best be described as recurring subluxation of the shoulder. The presenting complaint was of the shoulder 'going out of joint', but no significant trauma was recalled, dislocation was never shown on the radiograph and none required manual reduction. The only physical finding was apprehension on external rotation of the shoulder in abduction. Radiographs were frequently normal and arthrography and cineradiography were not helpful in confirming anterior displacement. The most useful preoperative information was obtained by manipulation of the shoulder under general anaesthesia just before the surgical repair. In all instances anterior instability could be demonstrated. Of 99 Magnuson-Stack repairs drawn from the records of the Vancouver General Hospital in a 3-year period, 34 proved to be examples of recurring subluxation. This high proportion of such patients contradicts the teaching in standard orthopaedic textbooks, but substantiates the warning of Rowe (1963) to beware of the patient whose shoulder 'dislocates' initially with little evidence of injury. It also substantiates Saha's concept (1971) of inherent shoulder instability as a contributor to the incidence of recurring dislocation."}
{"id": "PMID:478627", "title": "Entonox in the emergency department.", "content": "The use Entonox (BOC Medishield) was examined in a large accident and emergency department and was found to give satisfactory relief from pain in 92 per cent of cases. This compares favourably with reports of its results in other applications.", "contents": "Entonox in the emergency department. The use Entonox (BOC Medishield) was examined in a large accident and emergency department and was found to give satisfactory relief from pain in 92 per cent of cases. This compares favourably with reports of its results in other applications."}
{"id": "PMID:478628", "title": "Ankle injuries caused by seat belts.", "content": "Two cases of tri-malleolar frautures of the ankle caused by feet becoming entangled in loose seat belts are reported. Open reduction and internal fixation of all three malleolar fragments was carried out in each case with a satisfactory end result.", "contents": "Ankle injuries caused by seat belts. Two cases of tri-malleolar frautures of the ankle caused by feet becoming entangled in loose seat belts are reported. Open reduction and internal fixation of all three malleolar fragments was carried out in each case with a satisfactory end result."}
{"id": "PMID:478630", "title": "A six-month review of motorcycle accidents.", "content": "Ther were over 1000 fatal motorcycle accidents in Britain during 1976, which cost the taxpayer over pounds 40 million. There has been a 50 per cent increase in the number of motocyclists treated at Newcastle General Hospital Casualty Department over the past six months. Most accidents occur with young inexperienced male drivers, making elementary driving errors. Suggestions for positive prevention of motorcycle accidents are made. Of paramount importance is the need for consideration of compulsory formal training and enactment of the motorcycle lighting-up law.", "contents": "A six-month review of motorcycle accidents. Ther were over 1000 fatal motorcycle accidents in Britain during 1976, which cost the taxpayer over pounds 40 million. There has been a 50 per cent increase in the number of motocyclists treated at Newcastle General Hospital Casualty Department over the past six months. Most accidents occur with young inexperienced male drivers, making elementary driving errors. Suggestions for positive prevention of motorcycle accidents are made. Of paramount importance is the need for consideration of compulsory formal training and enactment of the motorcycle lighting-up law."}
{"id": "PMID:478632", "title": "Cell-mediated immune responses to the hemagglutinin and neuraminidase antigens of influenza A virus after immunization in humans.", "content": "Humoral and cell-mediated immunity (CMI) were evaluated in groups of school children after immunization with inactivated influenza virus vaccines. A conventional biphasic strain (H3ChN2Ch) of Port Chalmers influenza virus (X-41), a recombinant influenza virus specific for the neuraminidase antigen (Heq1N2Ch) of Port Chambers influenza A virus (X-42), and a placebo were employed for immunization. The techniques of hemagglutination inhibition and neuraminidase inhibition were used to determine serum antibody titers. The CMI responses were evaluated by the in vitro lymphocyte transformation assay employing HavN2Ch, Heq1Neq1, H3ChNeq1, and H3ChN2Ch influenza A virus strains as stimulants. Specific HAI antibody and CMI responses to H3Ch were observed in X-41 but not in X-42 vaccinees. Specific anti-neuraminidase antibodies and CMI responses to N2Ch were manifested by both X-41 and X-42 vaccinees. Immunization with the placebo resulted in no influenza-specific immune responses. The CMI response was first detectable 10 days after immunization and then declined. These observations demonstrate the induction of CMI responses to the HA and NA influenza surface antigens after immunization. These responses may be important in antiviral immunity and the recovery from influenza infection.", "contents": "Cell-mediated immune responses to the hemagglutinin and neuraminidase antigens of influenza A virus after immunization in humans. Humoral and cell-mediated immunity (CMI) were evaluated in groups of school children after immunization with inactivated influenza virus vaccines. A conventional biphasic strain (H3ChN2Ch) of Port Chalmers influenza virus (X-41), a recombinant influenza virus specific for the neuraminidase antigen (Heq1N2Ch) of Port Chambers influenza A virus (X-42), and a placebo were employed for immunization. The techniques of hemagglutination inhibition and neuraminidase inhibition were used to determine serum antibody titers. The CMI responses were evaluated by the in vitro lymphocyte transformation assay employing HavN2Ch, Heq1Neq1, H3ChNeq1, and H3ChN2Ch influenza A virus strains as stimulants. Specific HAI antibody and CMI responses to H3Ch were observed in X-41 but not in X-42 vaccinees. Specific anti-neuraminidase antibodies and CMI responses to N2Ch were manifested by both X-41 and X-42 vaccinees. Immunization with the placebo resulted in no influenza-specific immune responses. The CMI response was first detectable 10 days after immunization and then declined. These observations demonstrate the induction of CMI responses to the HA and NA influenza surface antigens after immunization. These responses may be important in antiviral immunity and the recovery from influenza infection."}
{"id": "PMID:478633", "title": "Immunological specificity of heat-stable opsonins in immune and nonimmune sera and their interaction with non-encapsulated and encapsulated strains of Staphylococcus aureus.", "content": "The in vitro interactions between strains of Staphylococcus aureus and human polymorphonuclear leukocytes in the presence of immune and nonimmune sera were studied. Evidence indicated that phagocytosis of encapsulated strains occurred in the presence of specific homologous antiserum, whereas non-encapsulated strains were readily phagocytized by polymorphonuclear leukocytes in the presence of both normal and immune sera. Immunological analyses demonstrated that normal serum opsonins, which reacted with the non-encapsulated strains, were specifically directed against exposed mucopeptide moieties of the organisms. Sera rich in antimucopeptide antibodies were obtained from rabbits immunized with heterologous bacteria such as Escherichia coli and group A-variant streptococci and were shown to be effective in opsonizing the non-encapsulated strains of S. aureus. Fresh clinical isolates of S. aureus were noticeably more resistant to the opsonizing effects of the antimucopeptide antibodies. Results were presented which suggest that the surface structures of these clinical isolates are more diverse than laboratory-propagated strains and that these antiphagocytic surface antigens may be significant factors in masking the opsonizing effects of the mucopeptide opsonins which are present in most sera.", "contents": "Immunological specificity of heat-stable opsonins in immune and nonimmune sera and their interaction with non-encapsulated and encapsulated strains of Staphylococcus aureus. The in vitro interactions between strains of Staphylococcus aureus and human polymorphonuclear leukocytes in the presence of immune and nonimmune sera were studied. Evidence indicated that phagocytosis of encapsulated strains occurred in the presence of specific homologous antiserum, whereas non-encapsulated strains were readily phagocytized by polymorphonuclear leukocytes in the presence of both normal and immune sera. Immunological analyses demonstrated that normal serum opsonins, which reacted with the non-encapsulated strains, were specifically directed against exposed mucopeptide moieties of the organisms. Sera rich in antimucopeptide antibodies were obtained from rabbits immunized with heterologous bacteria such as Escherichia coli and group A-variant streptococci and were shown to be effective in opsonizing the non-encapsulated strains of S. aureus. Fresh clinical isolates of S. aureus were noticeably more resistant to the opsonizing effects of the antimucopeptide antibodies. Results were presented which suggest that the surface structures of these clinical isolates are more diverse than laboratory-propagated strains and that these antiphagocytic surface antigens may be significant factors in masking the opsonizing effects of the mucopeptide opsonins which are present in most sera."}
{"id": "PMID:478634", "title": "Purification and characterization of Clostridium difficile toxin.", "content": "Recent evidence indicates that toxigenic Clostridium difficile strains are a major cause of antimicrobial-associated ileocecitis in laboratory animals and pseudomembranous colitis in humans. C. difficile ATCC 9689 was cultivated in a synthetic medium to which 3% ultrafiltrated proteose peptone was added. Purification of the toxin from broth filtrate was accomplished through ultrafiltration (100,000 nominal-molecular-weight-limit membrane), precipitation with 75% (NH4)2SO4, and chromatographic separation using Bio-Gel A 5m followed by ion-exchange chromatography on a diethylaminoethyl-Sephadex A-25 column. The purified toxin displayed only one band on polyacrylamide gel electrophoresis, and approximately 170 pg was cytopathic for human amnion cells. The isolated toxin was neutralized by Clostridium sordelli antitoxin, heat labile (56 degrees C for 30 min), and inactivated at pH 4 and 9; it had an isoelectric point of 5.0, increased vascular permeability in rabbits, and caused ileocecitis in hamsters when injected intracecally. Treatment of the toxin with trypsin, chymotrypsin, pronase, amylase, or ethylmercurithiosalicylate caused inactivation, whereas lipase had no effect. By gel filtration, its molecular weight was estimated as 530,000. Upon reduction and denaturation, the toxin dissociated into 185,000- and 50,000-molecular-weight components, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Extensive dissociation yielded only the 50,000-molecular-weight component. The toxin appears to be protoplasmic and is released into the surrounding environment upon autolysis of the cells. Attempts to correlate specific enzymatic activity with the toxin have been unsuccessful. These studies will help delineate the role of C. difficile toxin in antimicrobial-associated colitis and diarrhea.", "contents": "Purification and characterization of Clostridium difficile toxin. Recent evidence indicates that toxigenic Clostridium difficile strains are a major cause of antimicrobial-associated ileocecitis in laboratory animals and pseudomembranous colitis in humans. C. difficile ATCC 9689 was cultivated in a synthetic medium to which 3% ultrafiltrated proteose peptone was added. Purification of the toxin from broth filtrate was accomplished through ultrafiltration (100,000 nominal-molecular-weight-limit membrane), precipitation with 75% (NH4)2SO4, and chromatographic separation using Bio-Gel A 5m followed by ion-exchange chromatography on a diethylaminoethyl-Sephadex A-25 column. The purified toxin displayed only one band on polyacrylamide gel electrophoresis, and approximately 170 pg was cytopathic for human amnion cells. The isolated toxin was neutralized by Clostridium sordelli antitoxin, heat labile (56 degrees C for 30 min), and inactivated at pH 4 and 9; it had an isoelectric point of 5.0, increased vascular permeability in rabbits, and caused ileocecitis in hamsters when injected intracecally. Treatment of the toxin with trypsin, chymotrypsin, pronase, amylase, or ethylmercurithiosalicylate caused inactivation, whereas lipase had no effect. By gel filtration, its molecular weight was estimated as 530,000. Upon reduction and denaturation, the toxin dissociated into 185,000- and 50,000-molecular-weight components, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Extensive dissociation yielded only the 50,000-molecular-weight component. The toxin appears to be protoplasmic and is released into the surrounding environment upon autolysis of the cells. Attempts to correlate specific enzymatic activity with the toxin have been unsuccessful. These studies will help delineate the role of C. difficile toxin in antimicrobial-associated colitis and diarrhea."}
{"id": "PMID:478635", "title": "Flagella-induced immunity against experimental cholera in adult rabbits.", "content": "The adult rabbit ligated ileal loop model was used to evaluate the prophylactic potential of a crude flagellar (CF) vaccine produced from the classical. Inaba strain CA401. A greater than 1,000-fold increase in the challenge inoculum was required to induce an intestinal fluid response in actively immunized adult rabbits equivalent to that produced in unimmunized animals. Similar protection was afforded against challenge with classical and El Tor biotypes of both Inaba and Ogawa serotypes. Highly virulent 35S-labeled vibrios were inhibited in their ability to associated with the intestinal mucosa of CF-immunized rabbits. The protection conferred by CF immunization was found to be superior to that of a commercial bivalent vaccine and also to that of glutaraldehyde-treated cholera toxoid. The critical immunogenic component of CF appears to be a flagella-derived protein. The immunogenicity of CF was destroyed by heat treatment, and absorption of CF-immune serum with aflagellated mutant vibrios did not diminish its ability to confer a high level of passive protection. The intestinal protection of CF-immunized rabbits was completely reversed by the introduction of both goat anti-rabbit immunoglobulins A and G, but by neither alone.", "contents": "Flagella-induced immunity against experimental cholera in adult rabbits. The adult rabbit ligated ileal loop model was used to evaluate the prophylactic potential of a crude flagellar (CF) vaccine produced from the classical. Inaba strain CA401. A greater than 1,000-fold increase in the challenge inoculum was required to induce an intestinal fluid response in actively immunized adult rabbits equivalent to that produced in unimmunized animals. Similar protection was afforded against challenge with classical and El Tor biotypes of both Inaba and Ogawa serotypes. Highly virulent 35S-labeled vibrios were inhibited in their ability to associated with the intestinal mucosa of CF-immunized rabbits. The protection conferred by CF immunization was found to be superior to that of a commercial bivalent vaccine and also to that of glutaraldehyde-treated cholera toxoid. The critical immunogenic component of CF appears to be a flagella-derived protein. The immunogenicity of CF was destroyed by heat treatment, and absorption of CF-immune serum with aflagellated mutant vibrios did not diminish its ability to confer a high level of passive protection. The intestinal protection of CF-immunized rabbits was completely reversed by the introduction of both goat anti-rabbit immunoglobulins A and G, but by neither alone."}
{"id": "PMID:478639", "title": "Immunosuppression and isolation of rubella virus from human lymphocytes after vaccination with two rubella vaccines.", "content": "Two groups of young rubella-susceptible women were vaccinated with two rubella vaccines. Heparinized blood samples were taken from all individuals the day of vaccination and 5, 7, 15, 21, 30, 35, and 42 days later. Purified lymphocytes from these samples were cocultivated with AGMK cells for rubella virus isolation. Parallel samples of lymphocytes were stimulated with phytohemagglutinin, and the rate of [14C]thymidine incorporation was determined. Rubella virus was isolated from lymphocytes collected on days 7, 15, and 21 after RA27/3 vaccination in contrast to days 7 to 35 after HPV77 vaccination. The lymphocyte response to phytohemagglutinin was markedly suppressed from day 5 to 15. Normal lymphocyte responses were restored within 1 month after vaccination with RA27/3, but even later (1 week) after HPV77 vaccine. Lymphocytes from rubella-susceptible persons infected invitro with rubella virus vaccines and stimulated with phytohemagglutin displayed a decrease in their responsiveness to the mitogen similar to that observed with lymphocytes from vaccinees. The transient immunosuppression observed in vaccinees is probably due to virus-induced functional damage of the lymphocytes since no direct cytocidal effect of rubella vaccine has been demonstrated on human lymphocytes.", "contents": "Immunosuppression and isolation of rubella virus from human lymphocytes after vaccination with two rubella vaccines. Two groups of young rubella-susceptible women were vaccinated with two rubella vaccines. Heparinized blood samples were taken from all individuals the day of vaccination and 5, 7, 15, 21, 30, 35, and 42 days later. Purified lymphocytes from these samples were cocultivated with AGMK cells for rubella virus isolation. Parallel samples of lymphocytes were stimulated with phytohemagglutinin, and the rate of [14C]thymidine incorporation was determined. Rubella virus was isolated from lymphocytes collected on days 7, 15, and 21 after RA27/3 vaccination in contrast to days 7 to 35 after HPV77 vaccination. The lymphocyte response to phytohemagglutinin was markedly suppressed from day 5 to 15. Normal lymphocyte responses were restored within 1 month after vaccination with RA27/3, but even later (1 week) after HPV77 vaccine. Lymphocytes from rubella-susceptible persons infected invitro with rubella virus vaccines and stimulated with phytohemagglutin displayed a decrease in their responsiveness to the mitogen similar to that observed with lymphocytes from vaccinees. The transient immunosuppression observed in vaccinees is probably due to virus-induced functional damage of the lymphocytes since no direct cytocidal effect of rubella vaccine has been demonstrated on human lymphocytes."}
{"id": "PMID:478641", "title": "Efficacy of antimicrobial therapy in experimental rat pneumonia: antibiotic treatment schedules in rats with impaired phagocytosis.", "content": "Pneumococcal pneumonia in rats with intact host defense mechanisms could be successfully cured by penicillin. The efficacy of this antibiotic therapy was lost in cobra venom factor-treated rats which had selectively impaired phagocytic functions. In these animals the effect of penicillin therapy was improved by increasing the daily dose and the frequency of injections and by earlier initiation of the therapy. The efficacy of penicillin in the cobra venom facttor-treated rats was restored either by markedly increasing the daily dose of penicillin or by increasing the daily dose in combination with a reduced interval of the penicillin injections.", "contents": "Efficacy of antimicrobial therapy in experimental rat pneumonia: antibiotic treatment schedules in rats with impaired phagocytosis. Pneumococcal pneumonia in rats with intact host defense mechanisms could be successfully cured by penicillin. The efficacy of this antibiotic therapy was lost in cobra venom factor-treated rats which had selectively impaired phagocytic functions. In these animals the effect of penicillin therapy was improved by increasing the daily dose and the frequency of injections and by earlier initiation of the therapy. The efficacy of penicillin in the cobra venom facttor-treated rats was restored either by markedly increasing the daily dose of penicillin or by increasing the daily dose in combination with a reduced interval of the penicillin injections."}
{"id": "PMID:478636", "title": "Rabies virus immunity in genetically selected high- and low-responder lines of mice.", "content": "The antibody responsiveness to and the specific vaccination effect of rabies virus infection were investigated in high- and low-responder lines of mice produced by two-way selective breedings for quantitative production of antibodies to flagellar (H/f and L/f lines) or somatic (H/s and L/s lines) antigens of salmonellae. After specific immunization, both high lines were more resistant to rabies virus infection than were the low lines, and the protector effect was related to the level of antibody produced, as demonstrated by neutralizing serum activity. The present findings confirm the nonspecific genetic modification of the general antibody responsiveness induced in high- and low-responder lines selected for quantitative antibody production.", "contents": "Rabies virus immunity in genetically selected high- and low-responder lines of mice. The antibody responsiveness to and the specific vaccination effect of rabies virus infection were investigated in high- and low-responder lines of mice produced by two-way selective breedings for quantitative production of antibodies to flagellar (H/f and L/f lines) or somatic (H/s and L/s lines) antigens of salmonellae. After specific immunization, both high lines were more resistant to rabies virus infection than were the low lines, and the protector effect was related to the level of antibody produced, as demonstrated by neutralizing serum activity. The present findings confirm the nonspecific genetic modification of the general antibody responsiveness induced in high- and low-responder lines selected for quantitative antibody production."}
{"id": "PMID:478637", "title": "Animal model of neutropenia suitable for the study of dual-phagocyte systems.", "content": "When two sets of phagocytic cells participate simultaneously in the inflammatory process and bacterial killing, the relative contribution of each cell type is difficult to ascertain. The use of cell-specific antibody will permit selective depletion of one phagocyte population. We describe an experimental model of granulocytopenia which utilizes the immunoglobulin G fraction of an antigranulocyte serum. This material markedly depleted circulating polymorphonuclear leukocytes (PMN); within 2 h after injection of antigranulocyte globulin, PMN counts were at 19% of original levels and remained significantly depressed for 24 h. Granulocyte recruitment was also impaired, with only 5 x 10(3) PMN appearing in the lungs in response to an aerosol of Klebsiella, compared to 4.17 x 10(5) PMN in control animals (P less than 0.01). Most importantly, alveolar macrophages retained normal viability (97% versus 94% for control value, P not significant) normal phagocytic function, and normal bactericidal capacity. Antigranulocyte globulin is thus a valuable tool for the study of bacterial defense mechanisms.", "contents": "Animal model of neutropenia suitable for the study of dual-phagocyte systems. When two sets of phagocytic cells participate simultaneously in the inflammatory process and bacterial killing, the relative contribution of each cell type is difficult to ascertain. The use of cell-specific antibody will permit selective depletion of one phagocyte population. We describe an experimental model of granulocytopenia which utilizes the immunoglobulin G fraction of an antigranulocyte serum. This material markedly depleted circulating polymorphonuclear leukocytes (PMN); within 2 h after injection of antigranulocyte globulin, PMN counts were at 19% of original levels and remained significantly depressed for 24 h. Granulocyte recruitment was also impaired, with only 5 x 10(3) PMN appearing in the lungs in response to an aerosol of Klebsiella, compared to 4.17 x 10(5) PMN in control animals (P less than 0.01). Most importantly, alveolar macrophages retained normal viability (97% versus 94% for control value, P not significant) normal phagocytic function, and normal bactericidal capacity. Antigranulocyte globulin is thus a valuable tool for the study of bacterial defense mechanisms."}
{"id": "PMID:478638", "title": "Analysis of the effects of lipopolysaccharide on macrophages: differential phagocytic responses of C3H/HeN and C3H/HeJ macrophages in vitro.", "content": "The phenomenon of lipopolysaccharide (LPS)-induced in vitro macrophage cytotoxicity has been reported by a number of investigators but has often been difficult to reproduce and to quantitate. In this report, we have examined the effect of LPS on the ability of macrophages to ingest 51Cr-labeled, opsonized sheep erythrocytes as a method for examining the direct toxic effects of LPS on macrophages in vitro. By using this assy, we can clearly discriminate between LPS responder C3H/HeN macrophages and LPS nonresponder C3H/HeJ macrophages and demonstrate that LPS induces a profound inhibition of Fc-mediated phagocytosis in LPS responsive macrophages. Furthermore, low concentrations of LPS stimulate phagocytosis in macrophages derived for C3H/HeJ mice. The lipid A moiety of the LPS is responsible for the observed enhancement or inhibition of Fc-mediated phagocytosis. This assay was more sensitive than LPS-induced cytotoxicity, since inhibition of phagocytosis was detectable in cultures of LPS-sensitive macrophages even when cytotoxicity, assessed by trypan blue exclusion, was not. Thus, this assay represents an extremely sensitive method for analyzing the direct effects of LPS on macrophages.", "contents": "Analysis of the effects of lipopolysaccharide on macrophages: differential phagocytic responses of C3H/HeN and C3H/HeJ macrophages in vitro. The phenomenon of lipopolysaccharide (LPS)-induced in vitro macrophage cytotoxicity has been reported by a number of investigators but has often been difficult to reproduce and to quantitate. In this report, we have examined the effect of LPS on the ability of macrophages to ingest 51Cr-labeled, opsonized sheep erythrocytes as a method for examining the direct toxic effects of LPS on macrophages in vitro. By using this assy, we can clearly discriminate between LPS responder C3H/HeN macrophages and LPS nonresponder C3H/HeJ macrophages and demonstrate that LPS induces a profound inhibition of Fc-mediated phagocytosis in LPS responsive macrophages. Furthermore, low concentrations of LPS stimulate phagocytosis in macrophages derived for C3H/HeJ mice. The lipid A moiety of the LPS is responsible for the observed enhancement or inhibition of Fc-mediated phagocytosis. This assay was more sensitive than LPS-induced cytotoxicity, since inhibition of phagocytosis was detectable in cultures of LPS-sensitive macrophages even when cytotoxicity, assessed by trypan blue exclusion, was not. Thus, this assay represents an extremely sensitive method for analyzing the direct effects of LPS on macrophages."}
{"id": "PMID:478640", "title": "Efficacy of antimicrobial therapy in experimental rat pneumonia: effects of impaired phagocytosis.", "content": "The importance of intact host defense mechanisms for successful antimicrobial therapy was investigated in an animal model. Recovery from lobar pneumococcal pneumonia as a result of penicillin therapy was studied in normal rats and in rats treated with cobra venom factor. This factor was used to selectively suppress the phagocytosis of pneumococci as a result of complement depletion. Although complete recovery from the infection occurred in normal rats after appropriate penicillin therapy, this was not the case in cobra venom factor-treated rats. Within the limitations of this study, evidence is presented for loss of antibiotic activity as a consequence of impaired phagocytosis.", "contents": "Efficacy of antimicrobial therapy in experimental rat pneumonia: effects of impaired phagocytosis. The importance of intact host defense mechanisms for successful antimicrobial therapy was investigated in an animal model. Recovery from lobar pneumococcal pneumonia as a result of penicillin therapy was studied in normal rats and in rats treated with cobra venom factor. This factor was used to selectively suppress the phagocytosis of pneumococci as a result of complement depletion. Although complete recovery from the infection occurred in normal rats after appropriate penicillin therapy, this was not the case in cobra venom factor-treated rats. Within the limitations of this study, evidence is presented for loss of antibiotic activity as a consequence of impaired phagocytosis."}
{"id": "PMID:478642", "title": "Enhancement of generation of monocyte tissue thromboplastin by bacterial phagocytosis: possible pathway for fibrin formation on infected vegetations in bacterial endocarditis.", "content": "The deposition of fibrin on infected vegetations and the presence of mononuclear phagocytes that have phagocytized bacteria are remarkabe features in experimental bacterial endocarditis. In a study in vitro, we show that phagocytosis of bacteria by human monocytes enhances thromboplastin generation by these cells. Maximal enhancement of the generation of thromboplastin by monocytes was about six times compared with that in the control experiment without bacteria, and it was obtained by preincubation of the monocytes with 5 to 10 bacteria per monocyte. No quantitative difference was observed between Staphylococcus epidermidis and Streptococcus sanguis as to the enhancement of the monocyte thromboplastin generation. An enhancement of the procoagulant activity generation was also observed after addition of bacteria to human or rabbit whole blood. Probably, this generation was also due to synthesis of thromboplastin by monocytes. It is conceivable that fibrin deposition on infected vegetations during bacterial endocarditis is mediated by thromboplastin synthesis by monocytes.", "contents": "Enhancement of generation of monocyte tissue thromboplastin by bacterial phagocytosis: possible pathway for fibrin formation on infected vegetations in bacterial endocarditis. The deposition of fibrin on infected vegetations and the presence of mononuclear phagocytes that have phagocytized bacteria are remarkabe features in experimental bacterial endocarditis. In a study in vitro, we show that phagocytosis of bacteria by human monocytes enhances thromboplastin generation by these cells. Maximal enhancement of the generation of thromboplastin by monocytes was about six times compared with that in the control experiment without bacteria, and it was obtained by preincubation of the monocytes with 5 to 10 bacteria per monocyte. No quantitative difference was observed between Staphylococcus epidermidis and Streptococcus sanguis as to the enhancement of the monocyte thromboplastin generation. An enhancement of the procoagulant activity generation was also observed after addition of bacteria to human or rabbit whole blood. Probably, this generation was also due to synthesis of thromboplastin by monocytes. It is conceivable that fibrin deposition on infected vegetations during bacterial endocarditis is mediated by thromboplastin synthesis by monocytes."}
{"id": "PMID:478643", "title": "Infection of chicken erythrocytes with influenza and other viruses.", "content": "Chicken erythrocytes can be infected by the fowl plague (Rostock) strain (FP/R) of influenza type A, Newcastle disease virus (NDV), and Semliki Forest virus (SFV). Only NDV and SFV produced infectious progeny, albeit at low levels. Infection by FP/R was monitored by de novo synthesis of viral proteins, and the proteins synthesized could be identified by comparison with infected chicken fibroblast cells. FP/R synthesized far greater amounts of viral protein than did NDV or SFV.", "contents": "Infection of chicken erythrocytes with influenza and other viruses. Chicken erythrocytes can be infected by the fowl plague (Rostock) strain (FP/R) of influenza type A, Newcastle disease virus (NDV), and Semliki Forest virus (SFV). Only NDV and SFV produced infectious progeny, albeit at low levels. Infection by FP/R was monitored by de novo synthesis of viral proteins, and the proteins synthesized could be identified by comparison with infected chicken fibroblast cells. FP/R synthesized far greater amounts of viral protein than did NDV or SFV."}
{"id": "PMID:478644", "title": "Stimulation of peroxidase by chlamydial infection: cytochemistry of guinea pig conjunctival epithelium.", "content": "Guinea pig inclusion conjunctivitis, a naturally occurring chlamydial disease of guinea pigs, resolves spontaneously after 3 to 4 weeks. The factors responsible for curbing the infection have not yet been specifically defined. Since Iwata (Invest. Ophthalmol. 15:297-301, 1976) reported cytochemical activity for peroxidase in the conjunctival epithelium of the normal rat, we undertook these studies to determine whether a similar activity exists in the guinea pig, and if so, whether it functions in the elimination of this Chlamydia psittaci infection. Tarsal conjunctivas of 14 normal guinea pigs, 34 infected ones, and 7 control guinea pigs (inoculated with yolk sac only) were excised and tested for peroxidase by the Graham and Karnovsky method (J. Histochem. Cytochem. 14:291-302, 1966). We found that peroxidase activity, virtually absent in normal animals, was intensely stimulated by the infection. This enzymatic activity appeared 2 days after inoculation of the conjunctiva with chlamydia and persisted for 6 to 7 weeks. The enzyme was localized in the rough endoplasmic reticulum and perinuclear cisternae of all layers of the conjunctival epithelium from the external surface to the basal lamina, including cells with no apparent inclusions as well as those heavily parasitized. Reaction in the Golgi complex was variable. No reaction, however, was ever evident in the chlamydial vacuoles or lysosomes, and the organisms continued to grow and multiply during peak enzymatic activity. We therefore concluded that the stimulated enzyme is apparently not directly responsible for the waning of the infection, but instead reflects an alteration of host metabolism that occurs as a consequence of the infection.", "contents": "Stimulation of peroxidase by chlamydial infection: cytochemistry of guinea pig conjunctival epithelium. Guinea pig inclusion conjunctivitis, a naturally occurring chlamydial disease of guinea pigs, resolves spontaneously after 3 to 4 weeks. The factors responsible for curbing the infection have not yet been specifically defined. Since Iwata (Invest. Ophthalmol. 15:297-301, 1976) reported cytochemical activity for peroxidase in the conjunctival epithelium of the normal rat, we undertook these studies to determine whether a similar activity exists in the guinea pig, and if so, whether it functions in the elimination of this Chlamydia psittaci infection. Tarsal conjunctivas of 14 normal guinea pigs, 34 infected ones, and 7 control guinea pigs (inoculated with yolk sac only) were excised and tested for peroxidase by the Graham and Karnovsky method (J. Histochem. Cytochem. 14:291-302, 1966). We found that peroxidase activity, virtually absent in normal animals, was intensely stimulated by the infection. This enzymatic activity appeared 2 days after inoculation of the conjunctiva with chlamydia and persisted for 6 to 7 weeks. The enzyme was localized in the rough endoplasmic reticulum and perinuclear cisternae of all layers of the conjunctival epithelium from the external surface to the basal lamina, including cells with no apparent inclusions as well as those heavily parasitized. Reaction in the Golgi complex was variable. No reaction, however, was ever evident in the chlamydial vacuoles or lysosomes, and the organisms continued to grow and multiply during peak enzymatic activity. We therefore concluded that the stimulated enzyme is apparently not directly responsible for the waning of the infection, but instead reflects an alteration of host metabolism that occurs as a consequence of the infection."}
{"id": "PMID:478645", "title": "Immunization of mice against coccidioidomycosis with a subcellular vaccine.", "content": "In vitro-cultivated spherules of Coccidioides immitis were disrupted in a Braun homogenizer. The resultant wall fragments were separated from cell sap and endospores by centrifugation. Extraction of the walls with phosphate-buffered saline yielded a subcellular fraction that was immunogenic in mice. This fraction, which contained polysaccharide and protein, gave substantial protection against intranasal challenge with a usually lethal dose (1,000) of arthrospores, provided that it was administered with adjuvant.", "contents": "Immunization of mice against coccidioidomycosis with a subcellular vaccine. In vitro-cultivated spherules of Coccidioides immitis were disrupted in a Braun homogenizer. The resultant wall fragments were separated from cell sap and endospores by centrifugation. Extraction of the walls with phosphate-buffered saline yielded a subcellular fraction that was immunogenic in mice. This fraction, which contained polysaccharide and protein, gave substantial protection against intranasal challenge with a usually lethal dose (1,000) of arthrospores, provided that it was administered with adjuvant."}
{"id": "PMID:478646", "title": "Cytochalasin B does not inhibit ingestion of Chlamydia psittaci by mouse fibroblasts (L cells) and mouse peritoneal macrophages.", "content": "Cytochalasin B did not inhibit ingestion of Chlamydia psittaci by either mouse fibroblasts (L cells) or mouse peritoneal macrophages in concentrations that produced distinctive morphological changes and inhibited phagocytosis of polystyrene latex beads and Escherichia coli K-12.", "contents": "Cytochalasin B does not inhibit ingestion of Chlamydia psittaci by mouse fibroblasts (L cells) and mouse peritoneal macrophages. Cytochalasin B did not inhibit ingestion of Chlamydia psittaci by either mouse fibroblasts (L cells) or mouse peritoneal macrophages in concentrations that produced distinctive morphological changes and inhibited phagocytosis of polystyrene latex beads and Escherichia coli K-12."}
{"id": "PMID:478647", "title": "Interferon induction by the immunomodulating polyanion lambda carrageenan.", "content": "The lambda fraction of carrageenan induced murine interferon, which was characterized by sensitivity to trypsin and inhibition by pretreatment of target cells with actinomycin D.", "contents": "Interferon induction by the immunomodulating polyanion lambda carrageenan. The lambda fraction of carrageenan induced murine interferon, which was characterized by sensitivity to trypsin and inhibition by pretreatment of target cells with actinomycin D."}
{"id": "PMID:478648", "title": "Macrophage activation by bacterial cell walls and related synthetic compounds.", "content": "Activation of peritoneal macrophages from guinea pigs by various bacterial cell walls, M-1 endo-N-acetylmuramidase enzymatically digested bacterial cell walls and synthetic muramyl dipeptides was studied in terms of stimulation of [14C] glucosamine incorporation. All test bacterial cell wall preparations significantly increased a [14C]glucosamine uptake by the macrophages. Some of the water-soluble M-1 enzyme digests also exerted stimulating effects on macrophages, although the activity of the digests was found to be weaker than those of original cell walls. Furthermore, an adjuvant-active synthetic MurNAc-L-Ala-D-isoGln (MDP) showed a weak but significant activity, whereas an adjuvant-inactive analog, MurNAc-L-Ala-L-iso-Gln, did not show a significant activity, at least with the dose of 100 microgram. Additional studies with 6-O-acyl derivatives of MDP revealed that 6-O-(2-tetradecylhexadecanoyl)-MDP and 6-O-(3-hydroxy-2-tetradecyl-octadecanoyl)-MDP exhibit stronger macrophage-stimulating effects than MDP. It can be concluded from the above findings that MDP is the essential structure responsible for stimulating the activity of cell walls on guinea pig peritoneal macrophages, but it requires a particle state, which results from an additive character of lipophilicity, to exert the activity fully and effectively.", "contents": "Macrophage activation by bacterial cell walls and related synthetic compounds. Activation of peritoneal macrophages from guinea pigs by various bacterial cell walls, M-1 endo-N-acetylmuramidase enzymatically digested bacterial cell walls and synthetic muramyl dipeptides was studied in terms of stimulation of [14C] glucosamine incorporation. All test bacterial cell wall preparations significantly increased a [14C]glucosamine uptake by the macrophages. Some of the water-soluble M-1 enzyme digests also exerted stimulating effects on macrophages, although the activity of the digests was found to be weaker than those of original cell walls. Furthermore, an adjuvant-active synthetic MurNAc-L-Ala-D-isoGln (MDP) showed a weak but significant activity, whereas an adjuvant-inactive analog, MurNAc-L-Ala-L-iso-Gln, did not show a significant activity, at least with the dose of 100 microgram. Additional studies with 6-O-acyl derivatives of MDP revealed that 6-O-(2-tetradecylhexadecanoyl)-MDP and 6-O-(3-hydroxy-2-tetradecyl-octadecanoyl)-MDP exhibit stronger macrophage-stimulating effects than MDP. It can be concluded from the above findings that MDP is the essential structure responsible for stimulating the activity of cell walls on guinea pig peritoneal macrophages, but it requires a particle state, which results from an additive character of lipophilicity, to exert the activity fully and effectively."}
{"id": "PMID:478649", "title": "Use of HeLa cell guanine nucleotides by Chlamydia psittaci.", "content": "Exogenous guanine was found to be incorporated into the nucleic acids of Chlamydia psittaci when the parasite was grown in HeLa cells containing hypoxanthine guanine phosphoribosyltransferase (EC 2.4.2.8) activity but not when the parasite was grown in transferase-deficient HeLa cells. No evidence for a chlamydia-specific transferase activity was found in either transferase-containing or transferase-deficient infected HeLa cells. It is concluded that C. psittaci is incapable of metabolizing guanine, but that the parasite can use host-generated guanine nucleotides as precursors for nucleic acid synthesis.", "contents": "Use of HeLa cell guanine nucleotides by Chlamydia psittaci. Exogenous guanine was found to be incorporated into the nucleic acids of Chlamydia psittaci when the parasite was grown in HeLa cells containing hypoxanthine guanine phosphoribosyltransferase (EC 2.4.2.8) activity but not when the parasite was grown in transferase-deficient HeLa cells. No evidence for a chlamydia-specific transferase activity was found in either transferase-containing or transferase-deficient infected HeLa cells. It is concluded that C. psittaci is incapable of metabolizing guanine, but that the parasite can use host-generated guanine nucleotides as precursors for nucleic acid synthesis."}
{"id": "PMID:478651", "title": "The sensitivity of gentamicin-resistant gram-negative bacilli to cefotaxime, other cephalosporins and aminoglycosides.", "content": "The sensitivities of 80 gentamicin-resistant gram-negative bacilli to cefotaxime, cefuroxime, cefoxitin, cefamandole, cefazolin, tobramycin, netilmicin and amikacin were determined. Amikacin was the most active amino-glycoside. However, the percentage sensitivity to cefotaxime of most of the species was higher than, or equal to any of the other antibiotics tested. Cefotaxime was particulary active against Providencia spp., Serratia spp., Klebsiella spp., and Pseudomonas maltophilia, being 16 to 256 times more active than the next best cephalosporin or cephamycin. Clinical trials of cefotaxime are now required.", "contents": "The sensitivity of gentamicin-resistant gram-negative bacilli to cefotaxime, other cephalosporins and aminoglycosides. The sensitivities of 80 gentamicin-resistant gram-negative bacilli to cefotaxime, cefuroxime, cefoxitin, cefamandole, cefazolin, tobramycin, netilmicin and amikacin were determined. Amikacin was the most active amino-glycoside. However, the percentage sensitivity to cefotaxime of most of the species was higher than, or equal to any of the other antibiotics tested. Cefotaxime was particulary active against Providencia spp., Serratia spp., Klebsiella spp., and Pseudomonas maltophilia, being 16 to 256 times more active than the next best cephalosporin or cephamycin. Clinical trials of cefotaxime are now required."}
{"id": "PMID:478650", "title": "Comparative study of the binding of acylureidopenicillins and carbenicillin to human serum proteins.", "content": "The binding of the acylureidopenicillins azlocillin and mezlocillin to serum proteins was investigated by means of equilibrium dialysis and ultracentrifugation. The penicillin concentrations were determined by the agar diffusion test and by circular dichroism. The degree of binding is dependent on the penicillin concentration. At a mean penicillin concentration of 20 microgram/ml 39% of azlocillin is bound by serum proteins and 35% of mezlocillin. The acylureidopenicillins are bound to about the same degree by the two proteins albumin and gamma globulin, which occur in different concentrations in serum. The binding affinity delta F degree for the interaction between azlocillin and mezlocillin to human albumin is equal to -15,389 J/mol. The corresponding values for the binding of ampicillin and carbenicillin are -17,340 and -16,800 J/mol. Albumin has one binding site of a high affinity and two binding sites of a low affinity for the acylureidopenicillins. Measurements of partition coefficients of the penicillins for isobutanol and aqueous solution show that the interaction between acylureidopenicillins and serum is mainly hydrophobic in nature. This can be deduced from the fact that for example in the presence of sisomicin azlocillin is not displaced from its albumin binding site, in contrast to carbenicillin. The reason for this difference in behaviour lies in the fact that carbenicillin, like sisomicin, is bound to the serum proteins mainly by ionic bonds. In the case of azlocillin, however, hydrophobic bounds predominate which are not influenced by the ionic bonds of sisomicin.", "contents": "Comparative study of the binding of acylureidopenicillins and carbenicillin to human serum proteins. The binding of the acylureidopenicillins azlocillin and mezlocillin to serum proteins was investigated by means of equilibrium dialysis and ultracentrifugation. The penicillin concentrations were determined by the agar diffusion test and by circular dichroism. The degree of binding is dependent on the penicillin concentration. At a mean penicillin concentration of 20 microgram/ml 39% of azlocillin is bound by serum proteins and 35% of mezlocillin. The acylureidopenicillins are bound to about the same degree by the two proteins albumin and gamma globulin, which occur in different concentrations in serum. The binding affinity delta F degree for the interaction between azlocillin and mezlocillin to human albumin is equal to -15,389 J/mol. The corresponding values for the binding of ampicillin and carbenicillin are -17,340 and -16,800 J/mol. Albumin has one binding site of a high affinity and two binding sites of a low affinity for the acylureidopenicillins. Measurements of partition coefficients of the penicillins for isobutanol and aqueous solution show that the interaction between acylureidopenicillins and serum is mainly hydrophobic in nature. This can be deduced from the fact that for example in the presence of sisomicin azlocillin is not displaced from its albumin binding site, in contrast to carbenicillin. The reason for this difference in behaviour lies in the fact that carbenicillin, like sisomicin, is bound to the serum proteins mainly by ionic bonds. In the case of azlocillin, however, hydrophobic bounds predominate which are not influenced by the ionic bonds of sisomicin."}
{"id": "PMID:478652", "title": "Bactericidal or bacteristatic effects of two sulphonamide plus trimethoprim preparations in human urine.", "content": "Rrine collections were made by ten volunteers taking cotrimoxazole and a sulphamoxole/trimethoprim combination on a cross-over basis. The latter was given in approximately half the dose of cotrimoxazole. Following collection of urine and its sterile filtration, the trimethoprim and sulphonamide concentrations were estimated. The urines were then inoculated with various species of Enterobacteriaceae whose minimum inhibitory concentrations had been previously determined. The viable counts in these urines were followed for 24 hours and from these the times to kill 90% of bacteria were calculated. These were very reproducible for any one experiment but showed no correlation with drug concentration, source of the urine or organism sensitivity, except for one organism which had high resistance to both sulphonamide and trimethoprim. When the organism was sensitive to at least trimethoprim a slow bactericidal effect was generally seen with either combination. We concluded that in this type of experiment the higher dosed combination showed no advantage contrary to a previous report, but in agreement with another. This brings into question the current dosage regime of cotrimoxazole when used to treat urinary tract infections in that its higher dosage over certain other sulphonamide/trimethoprim combinations appears to confer no advantage in our experiments.", "contents": "Bactericidal or bacteristatic effects of two sulphonamide plus trimethoprim preparations in human urine. Rrine collections were made by ten volunteers taking cotrimoxazole and a sulphamoxole/trimethoprim combination on a cross-over basis. The latter was given in approximately half the dose of cotrimoxazole. Following collection of urine and its sterile filtration, the trimethoprim and sulphonamide concentrations were estimated. The urines were then inoculated with various species of Enterobacteriaceae whose minimum inhibitory concentrations had been previously determined. The viable counts in these urines were followed for 24 hours and from these the times to kill 90% of bacteria were calculated. These were very reproducible for any one experiment but showed no correlation with drug concentration, source of the urine or organism sensitivity, except for one organism which had high resistance to both sulphonamide and trimethoprim. When the organism was sensitive to at least trimethoprim a slow bactericidal effect was generally seen with either combination. We concluded that in this type of experiment the higher dosed combination showed no advantage contrary to a previous report, but in agreement with another. This brings into question the current dosage regime of cotrimoxazole when used to treat urinary tract infections in that its higher dosage over certain other sulphonamide/trimethoprim combinations appears to confer no advantage in our experiments."}
{"id": "PMID:478654", "title": "Propionibacterium acnes pneumonia in a patient with lymphoma.", "content": "A 57 year-old male with lymphocytic lymphoma developed symptoms of cough, fever and progressive dyspnea accompanied by a diffuse lower lung infiltrate. Open lung biopsy revealed multiple micro-abscesses surrounding small bronchioles which yielded a pure growth of Propionibacterium acnes.", "contents": "Propionibacterium acnes pneumonia in a patient with lymphoma. A 57 year-old male with lymphocytic lymphoma developed symptoms of cough, fever and progressive dyspnea accompanied by a diffuse lower lung infiltrate. Open lung biopsy revealed multiple micro-abscesses surrounding small bronchioles which yielded a pure growth of Propionibacterium acnes."}
{"id": "PMID:478653", "title": "[Tissue penetration of erythromycin (author's transl)].", "content": "Although erythromycin has been used in therapy for more than 25 years, until now there has been no paper summarizing the data on tissue penetration. The present review documents in detail dose- and time-related peak serum levels of erythromycin derivatives after oral administration, in addition to the erythromycin tissue levels. The erythromycin tissue levels often manifoldly exceed the corresponding MIC values of pathogens sensitive to erythromycin. However, erythromycin does not penetrate the central nervous system, or only penetrates in small, incalculable amounts. The same applies for the placental passage of erythromycin. The tissue levels confirm, with respect to the MIC values of the pathogen spectrum, clinical experience with erythromycin in the treatment of ear, nose and throat infections, respiratory tract infections and, with some limitations Chlamydia urethritis.", "contents": "[Tissue penetration of erythromycin (author's transl)]. Although erythromycin has been used in therapy for more than 25 years, until now there has been no paper summarizing the data on tissue penetration. The present review documents in detail dose- and time-related peak serum levels of erythromycin derivatives after oral administration, in addition to the erythromycin tissue levels. The erythromycin tissue levels often manifoldly exceed the corresponding MIC values of pathogens sensitive to erythromycin. However, erythromycin does not penetrate the central nervous system, or only penetrates in small, incalculable amounts. The same applies for the placental passage of erythromycin. The tissue levels confirm, with respect to the MIC values of the pathogen spectrum, clinical experience with erythromycin in the treatment of ear, nose and throat infections, respiratory tract infections and, with some limitations Chlamydia urethritis."}
{"id": "PMID:478655", "title": "[Legionnaires' disease in Germany (author's transl)].", "content": "In February 1979 a 51 year old man fell will in Munich, displaying symptoms of an influenza-like illness which developed into pneumonia. The patient died eight days later of circulatory collapse which failed to respond to treatment, accompanied by high temperature, leucopenia and agranulocytosis. Typical rods detected in the lung tissue and histological sections by immunofluorescence indicated the possibility of a Legionella pneumophila infection. The pathogen isolated from the lung tissue on CYE agar was identified as L. pneumophila, serogroup I. The diagnosis was confirmed by the CDC, Atlanta. This is the first time this organism has been isolated in Central Europe from a case with a fatal outcome.", "contents": "[Legionnaires' disease in Germany (author's transl)]. In February 1979 a 51 year old man fell will in Munich, displaying symptoms of an influenza-like illness which developed into pneumonia. The patient died eight days later of circulatory collapse which failed to respond to treatment, accompanied by high temperature, leucopenia and agranulocytosis. Typical rods detected in the lung tissue and histological sections by immunofluorescence indicated the possibility of a Legionella pneumophila infection. The pathogen isolated from the lung tissue on CYE agar was identified as L. pneumophila, serogroup I. The diagnosis was confirmed by the CDC, Atlanta. This is the first time this organism has been isolated in Central Europe from a case with a fatal outcome."}
{"id": "PMID:478659", "title": "Sex differences in daydreaming and related mental activity from the late teens to the early nineties.", "content": "Using the Imaginal Processes Inventory, aspects of daydreaming and associated mental activity were examined for sex differences in 1200 well educated middle and upper-middle class whites aged seventeen to ninety-two ninety-two years. Females reported higher levels of daydreaming and nightdreaming frequency and emotional reactions to daydreaming as well as more daydreams of a problem solving nature. Females also reported lower levels of daydreams of a sexual, bizarre-improbable, heroic and achievement-oriented nature. Most sex differences persisted over the life span with the difference for sexual daydreams increasing with increasing age level. Except for problem-solving daydreams, all daydreaming contents investigated decreased with increases in age. Across the lifespan problem-solving daydreams were the most likely for both sexes except for seventeen to twenty-nine year old males where such daydreams were second most likely; from age seventeen to twenty-nine sexual daydreams were most likely for males. After age forty, achievement oriented daydreams were relatively more important for females than for males. Females across the lifespan also reported more interpersonal curiosity than males while males exhibited more curiosity about things. Unexpectedly, male curiosity about things was equally as strong as their curiosity about people.", "contents": "Sex differences in daydreaming and related mental activity from the late teens to the early nineties. Using the Imaginal Processes Inventory, aspects of daydreaming and associated mental activity were examined for sex differences in 1200 well educated middle and upper-middle class whites aged seventeen to ninety-two ninety-two years. Females reported higher levels of daydreaming and nightdreaming frequency and emotional reactions to daydreaming as well as more daydreams of a problem solving nature. Females also reported lower levels of daydreams of a sexual, bizarre-improbable, heroic and achievement-oriented nature. Most sex differences persisted over the life span with the difference for sexual daydreams increasing with increasing age level. Except for problem-solving daydreams, all daydreaming contents investigated decreased with increases in age. Across the lifespan problem-solving daydreams were the most likely for both sexes except for seventeen to twenty-nine year old males where such daydreams were second most likely; from age seventeen to twenty-nine sexual daydreams were most likely for males. After age forty, achievement oriented daydreams were relatively more important for females than for males. Females across the lifespan also reported more interpersonal curiosity than males while males exhibited more curiosity about things. Unexpectedly, male curiosity about things was equally as strong as their curiosity about people."}
{"id": "PMID:478661", "title": "The outer limits of the welfare state: discrimination, racism and their effect on human services.", "content": "The European social democracies have been more generous than the United States in social provision, including services to aged. The momentum of provision has slowed down in recent years. We suggest that prosperity which has led to use of foreign laborers in menial jobs has caused this slow down. The dynamics are similar to the historical U.S. use of \"non-100% Americans\" as our menial workers. Changes in social policy strategies are needed: 1) universal provision, rather than programs aimed at minorities, is needed to enlist support of stable working and middle classes; 2) tax reforms, which lower progressive income-tax structures for middle incomes, and value-added tax, may be necessary to overcome anti-tax ideologies; 3) clear priorities as to most necessary services must be established by the gerontological community.", "contents": "The outer limits of the welfare state: discrimination, racism and their effect on human services. The European social democracies have been more generous than the United States in social provision, including services to aged. The momentum of provision has slowed down in recent years. We suggest that prosperity which has led to use of foreign laborers in menial jobs has caused this slow down. The dynamics are similar to the historical U.S. use of \"non-100% Americans\" as our menial workers. Changes in social policy strategies are needed: 1) universal provision, rather than programs aimed at minorities, is needed to enlist support of stable working and middle classes; 2) tax reforms, which lower progressive income-tax structures for middle incomes, and value-added tax, may be necessary to overcome anti-tax ideologies; 3) clear priorities as to most necessary services must be established by the gerontological community."}
{"id": "PMID:478660", "title": "Attitudes toward the elderly in Sweden: correlates and age group comparisons.", "content": "The objectives were to examine attitudes toward societal relations of the elderly in two age-stratified random samples, aged thirty to sixty-five, and seventy to seventy-five (both groups expressed attitudes toward the latter group), and to explore which of various correlates were associated with the attitudes. Six attitudinal dimensions were analyzed by means of group comparisons and linear regression. The younger group appeared positive toward interactive behavior, the elderly in turn being positive toward previous roles though reluctant to engagement in new activities. Some significant correlates were distinguished, although in a diversified manner over groups and dimensions: Positive attitudes among the younger were associated with low age, high education, and being female. No such clear pattern was found among the elderly, where instead work commitment and contacts with peers were particularly indicative of positive attitudes toward social activities.", "contents": "Attitudes toward the elderly in Sweden: correlates and age group comparisons. The objectives were to examine attitudes toward societal relations of the elderly in two age-stratified random samples, aged thirty to sixty-five, and seventy to seventy-five (both groups expressed attitudes toward the latter group), and to explore which of various correlates were associated with the attitudes. Six attitudinal dimensions were analyzed by means of group comparisons and linear regression. The younger group appeared positive toward interactive behavior, the elderly in turn being positive toward previous roles though reluctant to engagement in new activities. Some significant correlates were distinguished, although in a diversified manner over groups and dimensions: Positive attitudes among the younger were associated with low age, high education, and being female. No such clear pattern was found among the elderly, where instead work commitment and contacts with peers were particularly indicative of positive attitudes toward social activities."}
{"id": "PMID:478680", "title": "Recirculation dialysis (RD) (20 to 40 liters of dialysate) with venous bicarbonate buffering - an alternative procedure to hemofiltration (HF).", "content": "We report about a dialysis procedure, the circulation stability of which is comparable to hemofiltration treatment. Hemodynamic parameters were measured invasively and different procedures were compared for each patient. In RD and HF significant reduction in cardiac output (HF: --20%, RD: --21.5%), stroke volume (HF: --20%, RD: --17%), pulmonary artery pressure (HF: --57%, RD: --45:6%) and plasma volume (HF: --12%, RD: --15%) were found. Blood pressure and heart rate (HF: --1%, RD: --3%) remained stable despite the high utrafiltration rate. Peripheral resistance rose significantly (HF: + 20%, RD: + 25.9%). Compared with HG, the most important advantage during RD is the improved tolerance to treatment without typical hemodialysis-induced symptoms. In comparison to that, we found under conventional dialysis an inadequate increase in peripheral resistance (HD: + 6.1%). A following strong compensatory hyperventilation with drop in PCO2 was paralleled with typical symptoms like nausea and headache. Because of resttriction of this compensatory mechanism, hypotension and arrhythmia will be induced of the following peripheral tissue perfusion and central acidosis.", "contents": "Recirculation dialysis (RD) (20 to 40 liters of dialysate) with venous bicarbonate buffering - an alternative procedure to hemofiltration (HF). We report about a dialysis procedure, the circulation stability of which is comparable to hemofiltration treatment. Hemodynamic parameters were measured invasively and different procedures were compared for each patient. In RD and HF significant reduction in cardiac output (HF: --20%, RD: --21.5%), stroke volume (HF: --20%, RD: --17%), pulmonary artery pressure (HF: --57%, RD: --45:6%) and plasma volume (HF: --12%, RD: --15%) were found. Blood pressure and heart rate (HF: --1%, RD: --3%) remained stable despite the high utrafiltration rate. Peripheral resistance rose significantly (HF: + 20%, RD: + 25.9%). Compared with HG, the most important advantage during RD is the improved tolerance to treatment without typical hemodialysis-induced symptoms. In comparison to that, we found under conventional dialysis an inadequate increase in peripheral resistance (HD: + 6.1%). A following strong compensatory hyperventilation with drop in PCO2 was paralleled with typical symptoms like nausea and headache. Because of resttriction of this compensatory mechanism, hypotension and arrhythmia will be induced of the following peripheral tissue perfusion and central acidosis."}
{"id": "PMID:478681", "title": "Effects of 1-alpha OH D3 therapy in uremic patients in conservative or dialytic treatment.", "content": "The effects of phosphate restriction and of 1 alpha OH D3 administration were investigated in patients with advanced chronic renal failure. Few modifications of the various biochemical parameters in the patients were achieved with the restriction of dietary phosphate while better results were obtained with 1 alpha OH D3 administration. In dialyzed patients the treatment with this drug resulted in a normalization in serum calcium and alkaline phosphatase levels and in a remarkable significant decline in plasma parathyroid hormone and a reduction in the bone disease associated with uremia. This treatment in dialyzed uremic patients could avoid the employment of higher dialysate calcium concentration potentially dangerous for postdialysis hypercalcemia with the risk of metastatic calcifications.", "contents": "Effects of 1-alpha OH D3 therapy in uremic patients in conservative or dialytic treatment. The effects of phosphate restriction and of 1 alpha OH D3 administration were investigated in patients with advanced chronic renal failure. Few modifications of the various biochemical parameters in the patients were achieved with the restriction of dietary phosphate while better results were obtained with 1 alpha OH D3 administration. In dialyzed patients the treatment with this drug resulted in a normalization in serum calcium and alkaline phosphatase levels and in a remarkable significant decline in plasma parathyroid hormone and a reduction in the bone disease associated with uremia. This treatment in dialyzed uremic patients could avoid the employment of higher dialysate calcium concentration potentially dangerous for postdialysis hypercalcemia with the risk of metastatic calcifications."}
{"id": "PMID:478685", "title": "The exponential model for a regulatory enzyme: a computer program to determine the constants for the binding of a single ligand.", "content": "The paper describes a non-linear multiple regression program to analyse data arising from the binding of a single ligand by a protein under the assumption that the reaction can be represented by the exponential model for a regulatory enzyme.", "contents": "The exponential model for a regulatory enzyme: a computer program to determine the constants for the binding of a single ligand. The paper describes a non-linear multiple regression program to analyse data arising from the binding of a single ligand by a protein under the assumption that the reaction can be represented by the exponential model for a regulatory enzyme."}
{"id": "PMID:478686", "title": "A data storage and retrieval system for clinical research.", "content": "A data storage and retrieval system is presented that can be used for clinical research studies. The system is designed in such a way that the programs are independent of the application. It can be used by non-computer experts via a simple query language. Since it is bulit up in a modular way extensions can be easily made. The system is implemented on a PDP 11/70 under FORTRAN.", "contents": "A data storage and retrieval system for clinical research. A data storage and retrieval system is presented that can be used for clinical research studies. The system is designed in such a way that the programs are independent of the application. It can be used by non-computer experts via a simple query language. Since it is bulit up in a modular way extensions can be easily made. The system is implemented on a PDP 11/70 under FORTRAN."}
{"id": "PMID:478687", "title": "[Analytical study and physiological consequences of a model of respiration].", "content": "The author, starting from the pattern established by Brocas and Cherruault (1973), develops new equations by considering the dynamics of breathing. He distinguishes space without exhanges, called 'blower' and space with exchanges, called 'exchanger'. The equations are studied and explicit solutions are found by an original method. The necessity for a neutral gas is proved. The author develops periodic functions for ventilation, for gas pressures in the air and in the blood and control functions such as differences of pressure between aerial- and blood-dissolved gas at the bottom of the alveola, consumption of O2 and excretion of CO2 and remaining CO2 in blood. The control functions oscillate around zero for the differences and around constants for the others. Examining the effects of frequency of breathing, blood flow, variations of transerval diffusivities, atmospheric pressure, variation of the dimensions connected with fever, infections, altitude and depth, sclerosis and even emotions, he again determines the pathologies, their auscultation symptoms and other aspects.", "contents": "[Analytical study and physiological consequences of a model of respiration]. The author, starting from the pattern established by Brocas and Cherruault (1973), develops new equations by considering the dynamics of breathing. He distinguishes space without exhanges, called 'blower' and space with exchanges, called 'exchanger'. The equations are studied and explicit solutions are found by an original method. The necessity for a neutral gas is proved. The author develops periodic functions for ventilation, for gas pressures in the air and in the blood and control functions such as differences of pressure between aerial- and blood-dissolved gas at the bottom of the alveola, consumption of O2 and excretion of CO2 and remaining CO2 in blood. The control functions oscillate around zero for the differences and around constants for the others. Examining the effects of frequency of breathing, blood flow, variations of transerval diffusivities, atmospheric pressure, variation of the dimensions connected with fever, infections, altitude and depth, sclerosis and even emotions, he again determines the pathologies, their auscultation symptoms and other aspects."}
{"id": "PMID:478688", "title": "Results of simulation of non-homogeneous ventilatory mechanics for a patient-computer arrangement.", "content": "A model of the ventilatory mechanic system was realised on the MEDA TC 41 analogue computer; it consists of 2 structurally identical compartments, differing in the values of their coefficients. By changing the values of coefficients and the relations between the 2 compartments, it was possible to simulate the non-homogeneity of a real, pathologically-changed system of the body afflicted by disease. Complex resistance loops with phase shifts. identical with those really registered from patients, were realised; this was impossible for models with only 1 compartment. By changing the non-homogeneity of the model., it was possible to simulate the past and future development of the state of the diseased patient with sufficient reliability.", "contents": "Results of simulation of non-homogeneous ventilatory mechanics for a patient-computer arrangement. A model of the ventilatory mechanic system was realised on the MEDA TC 41 analogue computer; it consists of 2 structurally identical compartments, differing in the values of their coefficients. By changing the values of coefficients and the relations between the 2 compartments, it was possible to simulate the non-homogeneity of a real, pathologically-changed system of the body afflicted by disease. Complex resistance loops with phase shifts. identical with those really registered from patients, were realised; this was impossible for models with only 1 compartment. By changing the non-homogeneity of the model., it was possible to simulate the past and future development of the state of the diseased patient with sufficient reliability."}
{"id": "PMID:478689", "title": "The application of robust non-linear regression methods for fitting hyperbolic Scatchard plots.", "content": "The Scatchard plot is an essential tool for the estimation of binding parameters in biochemistry. In certain situations the fitting of a hyperbolic line is required in this context. It is proposed to solve this non-linear regression problem by robust methods. A method according to Huber and Dutter (1974) is outlined and its good applicability is demonstrated.", "contents": "The application of robust non-linear regression methods for fitting hyperbolic Scatchard plots. The Scatchard plot is an essential tool for the estimation of binding parameters in biochemistry. In certain situations the fitting of a hyperbolic line is required in this context. It is proposed to solve this non-linear regression problem by robust methods. A method according to Huber and Dutter (1974) is outlined and its good applicability is demonstrated."}
{"id": "PMID:478690", "title": "Cell-mediated cytotoxicity for cultured autologous melanoma cells.", "content": "Peripheral blood lymphocytes from 32 patients with malignant melanoma were tested for cell-mediated cytotoxicity (CMC) against cultured autologous melanoma cells. Effector cells were prepared from venous blood by defibrination, gel sedimentation, nylon column filtration, and lysis of remaining erythrocytes with NH4Cl. Melanoma cells prelabelled with [3H])proline were used as target cells in a 40-h assay and CMC was evaluated against standards obtained with blood lymphocytes from the least reactive normal donor. Reproducible autologous CMC was detected in 18 of 32 patients in a series of 367 total tests. CMC correlated with tumor volume (5-500 cm3) but not with tumor stage or DNCB reactivity. Preliminary results indicated that autologous CMC was not affected by treatment with DTIC, dexamethasone, intralesional BCG, radiation therapy, or partial surgical excision. Lack of consistent CMC in 14 patients could not be attributed to a measurable decrease in general immune capacity or to increased resistance of the patients' melanoma cells to CMC in general. Fibroblasts were more resistant to CMC than melanoma cells, and therefore of questionable value for defining specificity in direct tests.", "contents": "Cell-mediated cytotoxicity for cultured autologous melanoma cells. Peripheral blood lymphocytes from 32 patients with malignant melanoma were tested for cell-mediated cytotoxicity (CMC) against cultured autologous melanoma cells. Effector cells were prepared from venous blood by defibrination, gel sedimentation, nylon column filtration, and lysis of remaining erythrocytes with NH4Cl. Melanoma cells prelabelled with [3H])proline were used as target cells in a 40-h assay and CMC was evaluated against standards obtained with blood lymphocytes from the least reactive normal donor. Reproducible autologous CMC was detected in 18 of 32 patients in a series of 367 total tests. CMC correlated with tumor volume (5-500 cm3) but not with tumor stage or DNCB reactivity. Preliminary results indicated that autologous CMC was not affected by treatment with DTIC, dexamethasone, intralesional BCG, radiation therapy, or partial surgical excision. Lack of consistent CMC in 14 patients could not be attributed to a measurable decrease in general immune capacity or to increased resistance of the patients' melanoma cells to CMC in general. Fibroblasts were more resistant to CMC than melanoma cells, and therefore of questionable value for defining specificity in direct tests."}
{"id": "PMID:478691", "title": "Enhancement of carcinoembryonic antigen expression by interferon.", "content": "Human interferon decreased DNA but not RNA synthesis in a human colon carcinoma cell line, WiDr; in addition, there was a two- to three-fold increase in the expression of a tumor-associated antigen, carcinoembryonic antigen. In contrast, interferon had no effect on a normal human diploid cell line, WI-38. Thus, in addition to its anti-cellular effect against tumor cells, interferon can also modulate tumor antigenicity.", "contents": "Enhancement of carcinoembryonic antigen expression by interferon. Human interferon decreased DNA but not RNA synthesis in a human colon carcinoma cell line, WiDr; in addition, there was a two- to three-fold increase in the expression of a tumor-associated antigen, carcinoembryonic antigen. In contrast, interferon had no effect on a normal human diploid cell line, WI-38. Thus, in addition to its anti-cellular effect against tumor cells, interferon can also modulate tumor antigenicity."}
{"id": "PMID:478693", "title": "Inhibition of mammary tumorigenesis in GR mice with 2-bromo-alpha-ergocryptine.", "content": "A high incidence of mammary tumors is found in multiparous GR mice during the 2nd and 3rd pregnancies and in nulliparous GR mice treated with estrone/progesterone. The purpose of this study was to determine if prolactin is a contributing hormone in the genesis of these neoplasms. In one series of experiments, 238 15-week-old nulliparous GR mice were treated with estrone (drinking water, 0.5 mg/liter) plus progesterone (30 mg progesterone pellet with cholesterol, implanted SC once monthly) for a period of 13 weeks. Half of these mice were injected SC once daily with 100 micrograms of the prolactin-suppressing drug 2-bromo-alpha-ergocryptine (CB-154) for the duration of hormone treatment, and the other half were injected SC once daily with 0.9% NaCl solution (controls). In another series of experiments, 87 pregnant GR mice were divided into two groups and injected SC once daily from day 7 to 21 of pregnancy with 0.9% NaCl solution (controls) or CB-154 (100 micrograms/mouse). In the first series, the numbers of mice with mammary tumors and total number of mammary tumors were: controls, 58/119 (49%) and 73; CB-154 treatment, 34/119 (29%) and 37, respectively. In the second series, the numbers were: controls, 39/44 (89%) and 73; CB-154 treatment, 24/43 (56%) and 43, respectively. In both studies, CB-154 treatment significantly (p less than 0.05-0.005) reduced the percentage of mice with mammary tumors and total number of mammary tumors. These results provide evidence that prolactin is a contributing hormone in the genesis of estrone/progesterone and pregnancy-induced mammary tumors in female GR mice.", "contents": "Inhibition of mammary tumorigenesis in GR mice with 2-bromo-alpha-ergocryptine. A high incidence of mammary tumors is found in multiparous GR mice during the 2nd and 3rd pregnancies and in nulliparous GR mice treated with estrone/progesterone. The purpose of this study was to determine if prolactin is a contributing hormone in the genesis of these neoplasms. In one series of experiments, 238 15-week-old nulliparous GR mice were treated with estrone (drinking water, 0.5 mg/liter) plus progesterone (30 mg progesterone pellet with cholesterol, implanted SC once monthly) for a period of 13 weeks. Half of these mice were injected SC once daily with 100 micrograms of the prolactin-suppressing drug 2-bromo-alpha-ergocryptine (CB-154) for the duration of hormone treatment, and the other half were injected SC once daily with 0.9% NaCl solution (controls). In another series of experiments, 87 pregnant GR mice were divided into two groups and injected SC once daily from day 7 to 21 of pregnancy with 0.9% NaCl solution (controls) or CB-154 (100 micrograms/mouse). In the first series, the numbers of mice with mammary tumors and total number of mammary tumors were: controls, 58/119 (49%) and 73; CB-154 treatment, 34/119 (29%) and 37, respectively. In the second series, the numbers were: controls, 39/44 (89%) and 73; CB-154 treatment, 24/43 (56%) and 43, respectively. In both studies, CB-154 treatment significantly (p less than 0.05-0.005) reduced the percentage of mice with mammary tumors and total number of mammary tumors. These results provide evidence that prolactin is a contributing hormone in the genesis of estrone/progesterone and pregnancy-induced mammary tumors in female GR mice."}
{"id": "PMID:478694", "title": "Drug abuse, hopelessness, and suicidal behavior.", "content": "This study focused on assessing variables which may differentiate suicidal drug abusers from nonabusers. Data from a sample of drug abusing (n = 86) and nonabusing (n = 298) suicide attempters were subjected to a stepwise multiple regression analysis to identify factors related to suicidal intent. The analysis revealed that in our sample of suicide attempters, drug abuse status was not a significant contributor to the severity of suicidal intent. However, it was found that hopelessness accounted for a significant proportion of the variance of intent. The findings suggest that hopelessness among drug abusers, an important determinant of suicidal behavior, presents an important target for therapeutic intervention.", "contents": "Drug abuse, hopelessness, and suicidal behavior. This study focused on assessing variables which may differentiate suicidal drug abusers from nonabusers. Data from a sample of drug abusing (n = 86) and nonabusing (n = 298) suicide attempters were subjected to a stepwise multiple regression analysis to identify factors related to suicidal intent. The analysis revealed that in our sample of suicide attempters, drug abuse status was not a significant contributor to the severity of suicidal intent. However, it was found that hopelessness accounted for a significant proportion of the variance of intent. The findings suggest that hopelessness among drug abusers, an important determinant of suicidal behavior, presents an important target for therapeutic intervention."}
{"id": "PMID:478695", "title": "Role definition and confidentiality in the drug treatment process.", "content": "This national survey found that responses to confidentiality boundaries were determined by role definition. The extent to which confidentiality was viewed as a necessity depended upon the job that the individual held. Those individuals dealing with the drug abuser from an apprehension and corrections standpoint tended to see confidentiality in a limited sense, feeling that it pertained only to background material and medical records. Those in counseling positions saw confidentiality in much broader terms, in both its scope of protection and importance to the counseling situation.", "contents": "Role definition and confidentiality in the drug treatment process. This national survey found that responses to confidentiality boundaries were determined by role definition. The extent to which confidentiality was viewed as a necessity depended upon the job that the individual held. Those individuals dealing with the drug abuser from an apprehension and corrections standpoint tended to see confidentiality in a limited sense, feeling that it pertained only to background material and medical records. Those in counseling positions saw confidentiality in much broader terms, in both its scope of protection and importance to the counseling situation."}
{"id": "PMID:478696", "title": "Side effects in methadone patients: a survey of self-reported complaints.", "content": "Fifty-one patients in a methadone maintenance program completed a questionnaire containing 38 complaints upon admission and after 3 and 9 months in treatment. The questionnaire contained a graded scale of five possible responses from \"no problem\" to \"a very bad problem.\" Only three complaints at 3 months and five complaints at 9 months showed an increase in the percentage of clients reporting them as a problem compared to before methadone matinenance (BMM). When analyzed by summed mean, only one complaint at 3 months and two at 9 months showed an increase in severity on the graded scale compared to BMM. However, when each patient's responses were analyzed as to whether problems showed no change in severity, were improved, or increased in severity comparing BMM and after 9 months of methadone, 11 of 38 complaints showed a significant number of patients reporting them as more severe.", "contents": "Side effects in methadone patients: a survey of self-reported complaints. Fifty-one patients in a methadone maintenance program completed a questionnaire containing 38 complaints upon admission and after 3 and 9 months in treatment. The questionnaire contained a graded scale of five possible responses from \"no problem\" to \"a very bad problem.\" Only three complaints at 3 months and five complaints at 9 months showed an increase in the percentage of clients reporting them as a problem compared to before methadone matinenance (BMM). When analyzed by summed mean, only one complaint at 3 months and two at 9 months showed an increase in severity on the graded scale compared to BMM. However, when each patient's responses were analyzed as to whether problems showed no change in severity, were improved, or increased in severity comparing BMM and after 9 months of methadone, 11 of 38 complaints showed a significant number of patients reporting them as more severe."}
{"id": "PMID:478697", "title": "Urinary excretion of methadone during neonatal withdrawal: clinical application.", "content": "Methadone withdrawal manifestations in the neonate may be protracted and recur following therapy which suppresses symptoms. Sequential hemagglutination-inhibition measurements of methadone in urine can delineate the excretion period for this drug. A fall in titer to levels of 1:5 or less is associated with termination of the withdrawal process. The use of this technique serves as an adjunct to clinical assessment, particularly with regard to determining the length of time required for medical observation.", "contents": "Urinary excretion of methadone during neonatal withdrawal: clinical application. Methadone withdrawal manifestations in the neonate may be protracted and recur following therapy which suppresses symptoms. Sequential hemagglutination-inhibition measurements of methadone in urine can delineate the excretion period for this drug. A fall in titer to levels of 1:5 or less is associated with termination of the withdrawal process. The use of this technique serves as an adjunct to clinical assessment, particularly with regard to determining the length of time required for medical observation."}
{"id": "PMID:478698", "title": "A cognitive-behavioral versus nondirective group treatment program for opioid-addicted persons: an adjunct to methadone maintenance.", "content": "Fourteen methadone maintenance individuals participated in a 10-week program comparing the relative effectiveness of a cognitive-behavioral (CB) versus discussion-type (DISC) group in developing adaptive behavioral skills and self-evaluations. Posttreatment and follow-up results indicated that the CB group was significantly more successful than the DISC group in lowering reported levels of anxiety, depression, and situational non-assertiveness. These data are discussed in terms of a comprehensive, social rehabilitation program that involves multiple treatment techniques and measurement criteria.", "contents": "A cognitive-behavioral versus nondirective group treatment program for opioid-addicted persons: an adjunct to methadone maintenance. Fourteen methadone maintenance individuals participated in a 10-week program comparing the relative effectiveness of a cognitive-behavioral (CB) versus discussion-type (DISC) group in developing adaptive behavioral skills and self-evaluations. Posttreatment and follow-up results indicated that the CB group was significantly more successful than the DISC group in lowering reported levels of anxiety, depression, and situational non-assertiveness. These data are discussed in terms of a comprehensive, social rehabilitation program that involves multiple treatment techniques and measurement criteria."}
{"id": "PMID:478699", "title": "Personality characteristics of heroin addicts: a review of the empirical literature with critique--part I.", "content": "The empirical literature on personality characteristics of heroin addicts is reviewed. Studies using projective techniques, attribution theory, measures of needs values traits, and self-report inventories are summarized as they relate to heroin addicts. Summary statements are included in each section.", "contents": "Personality characteristics of heroin addicts: a review of the empirical literature with critique--part I. The empirical literature on personality characteristics of heroin addicts is reviewed. Studies using projective techniques, attribution theory, measures of needs values traits, and self-report inventories are summarized as they relate to heroin addicts. Summary statements are included in each section."}
{"id": "PMID:478700", "title": "Urban and rural Indian drinking patterns: the special case of the Lumbee.", "content": "This paper reports the results of a study of 460 adult male Lumbee Indians in a rural and urban setting. The study sought to determine what factors would explain the differences in drinking patterns between the rural and urban settings. The major variables considered were: (1) quantity, frequency, and variability of drinking; (2) occupational prestige; (3) commitment to work; (4) status satisfaction; and (5) job satisfaction. The urban Lumbee show a mean drinking pattern that is heavier than that of the rural group. In general, low achievement, as measured by occupational prestige and status satisfaction, and low satisfaction, as measured by job satisfaction and commitment to work, predict more excessive drinking patterns. Implications of the findings for treatment and future research are discussed.", "contents": "Urban and rural Indian drinking patterns: the special case of the Lumbee. This paper reports the results of a study of 460 adult male Lumbee Indians in a rural and urban setting. The study sought to determine what factors would explain the differences in drinking patterns between the rural and urban settings. The major variables considered were: (1) quantity, frequency, and variability of drinking; (2) occupational prestige; (3) commitment to work; (4) status satisfaction; and (5) job satisfaction. The urban Lumbee show a mean drinking pattern that is heavier than that of the rural group. In general, low achievement, as measured by occupational prestige and status satisfaction, and low satisfaction, as measured by job satisfaction and commitment to work, predict more excessive drinking patterns. Implications of the findings for treatment and future research are discussed."}
{"id": "PMID:478701", "title": "The \"German connection\": a survey of the antecedents and patterns of drug abuse in veterans returning from European service.", "content": "Questionnaire responses were obtained from 20 servicemen returning from European duty (Germany) enroute to their homes following discharge for drug dependency. A descriptive analysis is provided in terms of personal background, drug history, and motivating factors for initiation and continuation of drug use in Europe.", "contents": "The \"German connection\": a survey of the antecedents and patterns of drug abuse in veterans returning from European service. Questionnaire responses were obtained from 20 servicemen returning from European duty (Germany) enroute to their homes following discharge for drug dependency. A descriptive analysis is provided in terms of personal background, drug history, and motivating factors for initiation and continuation of drug use in Europe."}
{"id": "PMID:478702", "title": "Ethnicity and drug use among urban junior high school youths.", "content": "Data from a survey of youths attending a New York City junior high school were used to examine three perspectives of their drug involvement: (1) ethnic subculture, (2) social class, and (3) social adjustment. The results support the social adjustment view, although important differences were found in the demographic, attitude, and value correlates of drug involvement for the Black, Puerto Rican, and White students who were studied.", "contents": "Ethnicity and drug use among urban junior high school youths. Data from a survey of youths attending a New York City junior high school were used to examine three perspectives of their drug involvement: (1) ethnic subculture, (2) social class, and (3) social adjustment. The results support the social adjustment view, although important differences were found in the demographic, attitude, and value correlates of drug involvement for the Black, Puerto Rican, and White students who were studied."}
{"id": "PMID:478703", "title": "Pretreatment roles and therapeutic environment as correlates of in-treatment client \"success\": the case of the nonopiate user.", "content": "This study examined the effect of pretreatment role-involvements and therapeutic environment on the levels of in-treatment success for a sample of 704 nonopiate users. Within this sample, treatment type was found to be of little consequence in predicting success on selected measures of performance. The results of the analysis are interpreted as a function of theoretical difficulties in the paradigm that is used to organize the treatment effort.", "contents": "Pretreatment roles and therapeutic environment as correlates of in-treatment client \"success\": the case of the nonopiate user. This study examined the effect of pretreatment role-involvements and therapeutic environment on the levels of in-treatment success for a sample of 704 nonopiate users. Within this sample, treatment type was found to be of little consequence in predicting success on selected measures of performance. The results of the analysis are interpreted as a function of theoretical difficulties in the paradigm that is used to organize the treatment effort."}
{"id": "PMID:478704", "title": "Self-reported attitudes and behavior of Mexican-American drug use.", "content": "A drug usage study of students from a predominately Mexican-American population was conducted by comparing anonymously self-reported attitudinal and behavioral aspects of Mexican-American drug users and nonusers. Findings suggest M-A drug users and nonusers differ significantly in attitudes toward drug use and social behaviors, and users tend to be multiple drug users.", "contents": "Self-reported attitudes and behavior of Mexican-American drug use. A drug usage study of students from a predominately Mexican-American population was conducted by comparing anonymously self-reported attitudinal and behavioral aspects of Mexican-American drug users and nonusers. Findings suggest M-A drug users and nonusers differ significantly in attitudes toward drug use and social behaviors, and users tend to be multiple drug users."}
{"id": "PMID:478705", "title": "Personality characteristics of juvenile delinquent heroin users.", "content": "From a population of incarcerated delinquents coming from an urban minority community, 60 subjects with a history of heroin usage did not differ significantly from 60 controls on the Tennessee Self-Concept Scale, the Culture Fair Intelligence Test, or in reading grade level on the Wide Range Achievement Test. The two groups were also similar in intactness of family and type of offense. Controls were more deviant than users on the Alienation subscale of the Jesness Inventory (p less than .05). Unique traits of adolescent heroin users from a high risk area may not be found if social variables are controlled.", "contents": "Personality characteristics of juvenile delinquent heroin users. From a population of incarcerated delinquents coming from an urban minority community, 60 subjects with a history of heroin usage did not differ significantly from 60 controls on the Tennessee Self-Concept Scale, the Culture Fair Intelligence Test, or in reading grade level on the Wide Range Achievement Test. The two groups were also similar in intactness of family and type of offense. Controls were more deviant than users on the Alienation subscale of the Jesness Inventory (p less than .05). Unique traits of adolescent heroin users from a high risk area may not be found if social variables are controlled."}
{"id": "PMID:478706", "title": "A feminist approach for the treatment of drug-abusing women in a coed therapeutic community.", "content": "Experiences with female addicts in a feminist awareness group within a coed therapeutic community are reported. The group met for 16 months and was based on a variety of therapeutic techniques and educational presentations. The intervention resulted in more communication and trust among the women, increased interest in health-related concerns, a reduced sense of alienation, and gains in self-respect. The article discussed the issues and implications of resistance to the group, timing, development of indigenous leadership, training for feminist therapists, and the use of outside professional consultants in therapeutic communities.", "contents": "A feminist approach for the treatment of drug-abusing women in a coed therapeutic community. Experiences with female addicts in a feminist awareness group within a coed therapeutic community are reported. The group met for 16 months and was based on a variety of therapeutic techniques and educational presentations. The intervention resulted in more communication and trust among the women, increased interest in health-related concerns, a reduced sense of alienation, and gains in self-respect. The article discussed the issues and implications of resistance to the group, timing, development of indigenous leadership, training for feminist therapists, and the use of outside professional consultants in therapeutic communities."}
{"id": "PMID:478707", "title": "Drug-related suicide: a DAWN profile.", "content": "Drug-related suicides and suicide attempts/gestures account for a relatively large percentage of the drug-involved cases seen by hospital emergency rooms and medical examiners. While tranquilizer involvement characterizes the largest subgroup of suicide cases in the emergency room, barbiturate involvement is most frequently true of suicide cases seen by medical examiners. However, in both settings the suicides tend to be older and to include relatively more females and fewer Blacks than the nonsuicides.", "contents": "Drug-related suicide: a DAWN profile. Drug-related suicides and suicide attempts/gestures account for a relatively large percentage of the drug-involved cases seen by hospital emergency rooms and medical examiners. While tranquilizer involvement characterizes the largest subgroup of suicide cases in the emergency room, barbiturate involvement is most frequently true of suicide cases seen by medical examiners. However, in both settings the suicides tend to be older and to include relatively more females and fewer Blacks than the nonsuicides."}
{"id": "PMID:478708", "title": "Pupillary correlates of methadone treatment in heroin postaddicts.", "content": "The immediate effects of methadone intake on the pupillary response were examined with a sample of 15 postaddicts. Twenty heroin-related and 20 neutral words were presented auditorially immediately prior to and 35 minutes following the administration of methadone. The premethadone pupillary data failed to differentiate between the two groups of words. After administration, the pupil while responding initially in a similar manner to the two groups of words, returned to baseline more rapidly for the neutral words. The results suggest that withdrawal-induced arousal may not be entirely eliminated by the heroin substitute, methadone.", "contents": "Pupillary correlates of methadone treatment in heroin postaddicts. The immediate effects of methadone intake on the pupillary response were examined with a sample of 15 postaddicts. Twenty heroin-related and 20 neutral words were presented auditorially immediately prior to and 35 minutes following the administration of methadone. The premethadone pupillary data failed to differentiate between the two groups of words. After administration, the pupil while responding initially in a similar manner to the two groups of words, returned to baseline more rapidly for the neutral words. The results suggest that withdrawal-induced arousal may not be entirely eliminated by the heroin substitute, methadone."}
{"id": "PMID:478709", "title": "Maternal attitudes of alcoholic and nonalcoholic upper middle class women.", "content": "The Mother--Child Relationship Evaluation and the Maryland Parent Attitude Survey were administered to 30 alcoholic mothers and 30 nonalcoholic mothers. All subjects came from professional-managerial households and had two or more children between the ages of 8 and 18. The two groups differed significantly on both tests. The alcoholic women were less accepting, more rejecting, disciplinarian, or overprotecting, and they displayed a significantly greater degree of conflicting attitudes. This pattern resembles that found in alcoholic parents of either sex and of widely differing socioeconomic status.", "contents": "Maternal attitudes of alcoholic and nonalcoholic upper middle class women. The Mother--Child Relationship Evaluation and the Maryland Parent Attitude Survey were administered to 30 alcoholic mothers and 30 nonalcoholic mothers. All subjects came from professional-managerial households and had two or more children between the ages of 8 and 18. The two groups differed significantly on both tests. The alcoholic women were less accepting, more rejecting, disciplinarian, or overprotecting, and they displayed a significantly greater degree of conflicting attitudes. This pattern resembles that found in alcoholic parents of either sex and of widely differing socioeconomic status."}
{"id": "PMID:478710", "title": "Source of referral as an indicator of motivational factors and treatment outcome with drug-dependent clients.", "content": "Source of referral to substance abuse treatment agencies was investigated as a potential indicator of client motivation and treatment outcome. Clients referred by nine different sources were compared in terms of length of stay in treatment, drug use status at termination, and closing evaluation. There were no differences between the nine referral sources in terms of the frequency with which clients were rated as improved, no improvement, or recovered. However, there were significant differences between referral sources and length of time in treatment. Clients referred by their families or those who had prior institutional experience remained in treatment longer than other groups. Length of stay in treatment was related to drug use status at termination. Clients who had detoxified remained in treatment significantly longer than clients who had not detoxified. It may be that a social support system (family) and experience in a structured setting (prior institutional experience) facilitate positive treatment outcomes.", "contents": "Source of referral as an indicator of motivational factors and treatment outcome with drug-dependent clients. Source of referral to substance abuse treatment agencies was investigated as a potential indicator of client motivation and treatment outcome. Clients referred by nine different sources were compared in terms of length of stay in treatment, drug use status at termination, and closing evaluation. There were no differences between the nine referral sources in terms of the frequency with which clients were rated as improved, no improvement, or recovered. However, there were significant differences between referral sources and length of time in treatment. Clients referred by their families or those who had prior institutional experience remained in treatment longer than other groups. Length of stay in treatment was related to drug use status at termination. Clients who had detoxified remained in treatment significantly longer than clients who had not detoxified. It may be that a social support system (family) and experience in a structured setting (prior institutional experience) facilitate positive treatment outcomes."}
{"id": "PMID:478711", "title": "A socialization program for chronic alcoholics.", "content": "The staff of a court-mandated treatment program for convicted drunk drivers set up an alternative socialization program for chronic alcoholics who failed to respond to the defense-reductive strategies employed in their general program. No attempt was made to deal directly with long-standing underlying problems; rather emphasis was on improving social skills, thus reducing alcohol dependence as a social \"crutch\" or escape from social interaction. As social functioning increased, general behavior improved and drinking was reduced, with sober periods lengthened. Specific suggestions are given for a model program within a small agency context.", "contents": "A socialization program for chronic alcoholics. The staff of a court-mandated treatment program for convicted drunk drivers set up an alternative socialization program for chronic alcoholics who failed to respond to the defense-reductive strategies employed in their general program. No attempt was made to deal directly with long-standing underlying problems; rather emphasis was on improving social skills, thus reducing alcohol dependence as a social \"crutch\" or escape from social interaction. As social functioning increased, general behavior improved and drinking was reduced, with sober periods lengthened. Specific suggestions are given for a model program within a small agency context."}
{"id": "PMID:478712", "title": "Bias in drug abuse survey research.", "content": "An analysis of the drug abuse literature indicated that significant biases may affect the existing data. First, the lack of standardization in survey research leads to problems of reliability, validity, and objectivity in drug abuse measurement. Second, the \"demand characteristics\" of the survey situation may cause the subject to bias his responses in a particular direction, depending on his interaction with and his interpretations of the survey conditions. Third, both overt and covert biases of the researcher may significantly affect the outcome of the survey. Fourth, limitations of the survey method itself (e.g., the source of the survey data, difficulties in obtaining random samples, conclusions overemphasizing student drug use, limitations of the sample survey as a measurement device) seriously restrict our understanding of drug abuse phenomena.", "contents": "Bias in drug abuse survey research. An analysis of the drug abuse literature indicated that significant biases may affect the existing data. First, the lack of standardization in survey research leads to problems of reliability, validity, and objectivity in drug abuse measurement. Second, the \"demand characteristics\" of the survey situation may cause the subject to bias his responses in a particular direction, depending on his interaction with and his interpretations of the survey conditions. Third, both overt and covert biases of the researcher may significantly affect the outcome of the survey. Fourth, limitations of the survey method itself (e.g., the source of the survey data, difficulties in obtaining random samples, conclusions overemphasizing student drug use, limitations of the sample survey as a measurement device) seriously restrict our understanding of drug abuse phenomena."}
{"id": "PMID:478713", "title": "Alcoholism and the Bender-Gestalt Test.", "content": "Research with the Bender-Gestalt Test and alcoholism has focused on possible organic deficits in alcoholics and elucidation of hypothesized alcoholic personality characteristics. The evidence for both is equivocal.", "contents": "Alcoholism and the Bender-Gestalt Test. Research with the Bender-Gestalt Test and alcoholism has focused on possible organic deficits in alcoholics and elucidation of hypothesized alcoholic personality characteristics. The evidence for both is equivocal."}
{"id": "PMID:478714", "title": "Efficacy of a three- versus a five-week alcohol treatment program.", "content": "Eighty-six male alcoholic inpatients were randomly assigned to comparable 3- or 5-week treatment progrrams. Psychometric testing at the beginning and the end of treatment was supplemented by follow-up data 6 months after discharge. While both the 3- and 5-week groups demonstrated significant improvement on both MMPI and follow-up data, few differences between the groups were revealed. The comparable results obtained offer little justification for prolonging inpatient treatment beyond 3 weeks. It is suggested that the inpatient phase of alcohol treatment be brief and oriented to developing a well-structured extended outpatient program.", "contents": "Efficacy of a three- versus a five-week alcohol treatment program. Eighty-six male alcoholic inpatients were randomly assigned to comparable 3- or 5-week treatment progrrams. Psychometric testing at the beginning and the end of treatment was supplemented by follow-up data 6 months after discharge. While both the 3- and 5-week groups demonstrated significant improvement on both MMPI and follow-up data, few differences between the groups were revealed. The comparable results obtained offer little justification for prolonging inpatient treatment beyond 3 weeks. It is suggested that the inpatient phase of alcohol treatment be brief and oriented to developing a well-structured extended outpatient program."}
{"id": "PMID:478715", "title": "Exploration of a live-together-group-together paradigm with incarcerated drug offenders.", "content": "Prisoners who both participated in biweekly, biracial therapy groups and lived together in large cubicles were compared with prisoners who grouped but did not live together. Results suggested: (1) greater group cohesion among inmates who lived and grouped together, (2) greater acceptance of Blacks by Whites exposed to the cubicle system, and (3) that the structure of measures used in studies of this nature may affect results appreciably. Implications for correctional treatment and future research are discussed.", "contents": "Exploration of a live-together-group-together paradigm with incarcerated drug offenders. Prisoners who both participated in biweekly, biracial therapy groups and lived together in large cubicles were compared with prisoners who grouped but did not live together. Results suggested: (1) greater group cohesion among inmates who lived and grouped together, (2) greater acceptance of Blacks by Whites exposed to the cubicle system, and (3) that the structure of measures used in studies of this nature may affect results appreciably. Implications for correctional treatment and future research are discussed."}
{"id": "PMID:478716", "title": "University infirmary admissions for drug abuse, 1968--1970 and 1973--1975.", "content": "University overnight infirmary admissions for drug abuse were reviewed over the 2-year periods 1968--1970 and 1973--1975. Hallucinogen abuse diminished over the years studied while alcohol abuse increased. Marijuana abuse was essentially unchanged. Diazepam overdosage generally replaced barbiturate overdosage, while aspirin remained the most common drug in overdose situations.", "contents": "University infirmary admissions for drug abuse, 1968--1970 and 1973--1975. University overnight infirmary admissions for drug abuse were reviewed over the 2-year periods 1968--1970 and 1973--1975. Hallucinogen abuse diminished over the years studied while alcohol abuse increased. Marijuana abuse was essentially unchanged. Diazepam overdosage generally replaced barbiturate overdosage, while aspirin remained the most common drug in overdose situations."}
{"id": "PMID:478717", "title": "Psychoanalytical studies on the causes of intoxication by marijuana.", "content": "The study of 27 illegal users of marijuana, through psychoanalysis and analytical group psychotherapy and through psychiatric examinations, made it possible to discover that these patients felt their parents to be aggressive or indifferent or unaffectionate or absent (among the parents of different temperaments, and confirmed by statistical data). These feelings were related to the errors committed by their parents during the molding periods (lactation and family environment).", "contents": "Psychoanalytical studies on the causes of intoxication by marijuana. The study of 27 illegal users of marijuana, through psychoanalysis and analytical group psychotherapy and through psychiatric examinations, made it possible to discover that these patients felt their parents to be aggressive or indifferent or unaffectionate or absent (among the parents of different temperaments, and confirmed by statistical data). These feelings were related to the errors committed by their parents during the molding periods (lactation and family environment)."}
{"id": "PMID:478723", "title": "Electrostimulation of the median nerve in tetraplegics by means of implanted stimulators.", "content": "Since 1971 we studied on the possibility of utilization of implanted stimulators in tetraplegics caused by spinal cord injury of the cervical segment. Restoration to tetraplegic patients of the ability to perform some grasping functions of the hand may make them recover some independence and reduce the degree of disability. The examination conducted so far supplied much valuable information regarding reactions of a neuromuscular system at implanted stimulators as well the influence of systematic stimulation training on functions of the hand. This was not observed by other authors using transcutaneous stimulation. The examinations we performed revealed a several-fold increase in the force and prolongation of the period of maintenance of the maximal moment developed by the stimulation as well as the result of the stimulation training. Another observation we made was the possibility of active influence of the patient on the stimulation results. It consists in the possibility of active increase by the patient of the value of forces developed by the stimulated, previously paralysed muscles. The effect on the stimulation level, and on the extent of the \"stimulation base\" was found. The effect of active \"support\" of stimulation by the patient is the more effective, the lower the stimulation base is. We believe that our observations may have practical significance. Perfection of control over the stimulators' activity from inner sources will probably permit in many instances to utilize functionally tetraplegics' paralysed hand.", "contents": "Electrostimulation of the median nerve in tetraplegics by means of implanted stimulators. Since 1971 we studied on the possibility of utilization of implanted stimulators in tetraplegics caused by spinal cord injury of the cervical segment. Restoration to tetraplegic patients of the ability to perform some grasping functions of the hand may make them recover some independence and reduce the degree of disability. The examination conducted so far supplied much valuable information regarding reactions of a neuromuscular system at implanted stimulators as well the influence of systematic stimulation training on functions of the hand. This was not observed by other authors using transcutaneous stimulation. The examinations we performed revealed a several-fold increase in the force and prolongation of the period of maintenance of the maximal moment developed by the stimulation as well as the result of the stimulation training. Another observation we made was the possibility of active influence of the patient on the stimulation results. It consists in the possibility of active increase by the patient of the value of forces developed by the stimulated, previously paralysed muscles. The effect on the stimulation level, and on the extent of the \"stimulation base\" was found. The effect of active \"support\" of stimulation by the patient is the more effective, the lower the stimulation base is. We believe that our observations may have practical significance. Perfection of control over the stimulators' activity from inner sources will probably permit in many instances to utilize functionally tetraplegics' paralysed hand."}
{"id": "PMID:478724", "title": "Technical and clinical function testing of hand orthoses in Sweden.", "content": "The technical and clinical function testing of hand orthoses by the Swedish institute for the Handicapped at EFTO (the Unit of Applied Orthotics) is performed according to the test instructions for each specific hand orthosis, wrist-driven, finger-driven, etc. The clinical testing follows a specific routine where a medical record of the patients with the specific questions regarding the hand orthoses are noted; diagnosis, function loss, psychological questions, technical aids, etc. The improvement in function and patient independence is checked against the list of Activities of Daily Living. The technical test follows test instructions specially designed to indicate the special characteristics of the type of orthosis which is to be tested. The technical type test includes an examination and dimensional inspection, mechanical, climatic and durability tests. Our test records are checked with our requirement specifications for each type of aid. When our requirements are fulfilled, the orthosis is recommended. 6 of 9 commercially available wrist-driven hand orthosis have been tested. Only 2 are recommended for prescription: They are from Orthotic System, USA, and from Jaeco Orthopedic Specialties, USA. Two finger-driven hand orthoses have been tested and recommended for prescription: One from Orthomedics Inc., USA, and one from Jaeco Orthopedic Specialties. Three electrical power units for hand orthoses have been tested and two were recommended. The two are from Hosmer/Dorrance, USA, and from EEN-Holmgren Orthopedic Inc., Sweden. One self-contained hand orthosis powered with the Een-Holmgren Actuating Unit has been tested and recommended.", "contents": "Technical and clinical function testing of hand orthoses in Sweden. The technical and clinical function testing of hand orthoses by the Swedish institute for the Handicapped at EFTO (the Unit of Applied Orthotics) is performed according to the test instructions for each specific hand orthosis, wrist-driven, finger-driven, etc. The clinical testing follows a specific routine where a medical record of the patients with the specific questions regarding the hand orthoses are noted; diagnosis, function loss, psychological questions, technical aids, etc. The improvement in function and patient independence is checked against the list of Activities of Daily Living. The technical test follows test instructions specially designed to indicate the special characteristics of the type of orthosis which is to be tested. The technical type test includes an examination and dimensional inspection, mechanical, climatic and durability tests. Our test records are checked with our requirement specifications for each type of aid. When our requirements are fulfilled, the orthosis is recommended. 6 of 9 commercially available wrist-driven hand orthosis have been tested. Only 2 are recommended for prescription: They are from Orthotic System, USA, and from Jaeco Orthopedic Specialties, USA. Two finger-driven hand orthoses have been tested and recommended for prescription: One from Orthomedics Inc., USA, and one from Jaeco Orthopedic Specialties. Three electrical power units for hand orthoses have been tested and two were recommended. The two are from Hosmer/Dorrance, USA, and from EEN-Holmgren Orthopedic Inc., Sweden. One self-contained hand orthosis powered with the Een-Holmgren Actuating Unit has been tested and recommended."}
{"id": "PMID:478725", "title": "Teaching toothbrushing behavior in severely retarded adults: systematic reduction of feedback and duration training.", "content": "Eight severely retarded adults were trained to brush their teeth. Component behaviours were trained in a predetermined sequence of 15 steps. Training included scheduled opportunities for independence performance, instruction, modelling and partial and total physical guidance. Four types of feedback were used, i.e., verbal praise plus explicit head nodding, explicit nodding, inconspicous nodding and no feedback. Stepwise reduction of feedback was made contingent on a subject's repeated performance of each individual step. Five of the eight subjects learned to perform all 15 steps for three consecutive sessions. A different procedure for fading out feedback was used for the other three subjects, two of which learned to perform 14 steps reliably. Acquisition training was followed by procedures to establish persistance of criterion performances for an interval of eight days. Training procedures were based on interval shaping. All seven subjects learned to demonstrate the 14 or 15 component behaviours after an interval of eight to 17 days in which no systematic training occured.", "contents": "Teaching toothbrushing behavior in severely retarded adults: systematic reduction of feedback and duration training. Eight severely retarded adults were trained to brush their teeth. Component behaviours were trained in a predetermined sequence of 15 steps. Training included scheduled opportunities for independence performance, instruction, modelling and partial and total physical guidance. Four types of feedback were used, i.e., verbal praise plus explicit head nodding, explicit nodding, inconspicous nodding and no feedback. Stepwise reduction of feedback was made contingent on a subject's repeated performance of each individual step. Five of the eight subjects learned to perform all 15 steps for three consecutive sessions. A different procedure for fading out feedback was used for the other three subjects, two of which learned to perform 14 steps reliably. Acquisition training was followed by procedures to establish persistance of criterion performances for an interval of eight days. Training procedures were based on interval shaping. All seven subjects learned to demonstrate the 14 or 15 component behaviours after an interval of eight to 17 days in which no systematic training occured."}
{"id": "PMID:478736", "title": "Interdisciplinary cooperation in an integrated psychiatric rehabilitation program.", "content": "The development of psychiatry is at present stormy with discussions dividing, polarising and partly paralysing. Current experience shows that psychotherapeutic approaches are not alternatives to psychopharmacological ones but necessary complements. An integrated therapy which treats the patient as a social, psychological and organic entity has not yet been spread to the poorly-equipped psychiatric periphery but is mainly restricted to the modern richly-endowed psychiatric centers. Modern psychiatric rehabilitation principles according to this integrated synoptic view seem however to be applicable even to custodial-treated patients at peripheral psychiatric institutions. In this article we present the special psycho-social, epidemiological, administrative and economical structure of the island of Gotland due to its geographical isolation. We describe the construction and forming of a rehabilitation program under greatly limited economical conditions. The program combines communication-oriented group therapy with consequent pharmacotherapy and a structured ergotherapeutic and ADL-therapeutic program and is applied to a primarily unselected sample of patients. We also describe the education and supervision of the staff as well as the components of our program. Positive results in spite of limited resources have been achieved. Problems are discussed.", "contents": "Interdisciplinary cooperation in an integrated psychiatric rehabilitation program. The development of psychiatry is at present stormy with discussions dividing, polarising and partly paralysing. Current experience shows that psychotherapeutic approaches are not alternatives to psychopharmacological ones but necessary complements. An integrated therapy which treats the patient as a social, psychological and organic entity has not yet been spread to the poorly-equipped psychiatric periphery but is mainly restricted to the modern richly-endowed psychiatric centers. Modern psychiatric rehabilitation principles according to this integrated synoptic view seem however to be applicable even to custodial-treated patients at peripheral psychiatric institutions. In this article we present the special psycho-social, epidemiological, administrative and economical structure of the island of Gotland due to its geographical isolation. We describe the construction and forming of a rehabilitation program under greatly limited economical conditions. The program combines communication-oriented group therapy with consequent pharmacotherapy and a structured ergotherapeutic and ADL-therapeutic program and is applied to a primarily unselected sample of patients. We also describe the education and supervision of the staff as well as the components of our program. Positive results in spite of limited resources have been achieved. Problems are discussed."}
{"id": "PMID:478737", "title": "[Antisociality and school failure as judged by elementary school teachers (author's transl)].", "content": "126 elementary school teachers were interviewed in respect to their concepts of school failure and antisociality. According to their opinions antisociality finds its expression especially in the following behaviour: delinquent and/or destructive actions against others or the state, deliberate disturbance of social peace and order in a society, and criminality. The main causes of school failure are seen in intellectual-emotional development retardation, social isolation, rejection of a child by its parents, insufficient maturity for education hereditary mental retardation and lack of intelligence. The teachers' appraisal of antisociality and school failure are largely influenced by implicit theories, by which \"the antisocial\" and \"the school failure\" are regarded as deficit personalities. The teachers are aware of the overrepresentation of children from fringe groups and low social classes among antisocial or educationally failing pupils, they do, however, not realistically recognize the respective causal relations. In difference to other social control agents, their judgements nearly deny that school failure may also be caused by teachers.", "contents": "[Antisociality and school failure as judged by elementary school teachers (author's transl)]. 126 elementary school teachers were interviewed in respect to their concepts of school failure and antisociality. According to their opinions antisociality finds its expression especially in the following behaviour: delinquent and/or destructive actions against others or the state, deliberate disturbance of social peace and order in a society, and criminality. The main causes of school failure are seen in intellectual-emotional development retardation, social isolation, rejection of a child by its parents, insufficient maturity for education hereditary mental retardation and lack of intelligence. The teachers' appraisal of antisociality and school failure are largely influenced by implicit theories, by which \"the antisocial\" and \"the school failure\" are regarded as deficit personalities. The teachers are aware of the overrepresentation of children from fringe groups and low social classes among antisocial or educationally failing pupils, they do, however, not realistically recognize the respective causal relations. In difference to other social control agents, their judgements nearly deny that school failure may also be caused by teachers."}
{"id": "PMID:478748", "title": "[Rapid diagnosis of carbon monoxide poisoning and quantitative determination of CO-Hb in blood].", "content": "A quick diagnosis of carbon monoxide poisoning is a very difficult clinical problem. This paper describes a photometric method which permits a reliable diagnosis in a time of 7 to 10 minutes. Only 0.3 ml whole blood are necessary for the total procedure. Carboxyhemoglobin changes in absorption maximum slightly after reduction, whereas Oxyhemoglobin shows a significant change in extinction. Measurement of the hemolysate at the same wave length before and after alkaline reduction results in large differences in the extinctions (very large for Oxyhemoglobin and very small for Carboxyhemoglobin). Calibration curves are prepared by calibrating rest-extinction defined as: (formula: see text) to percent Carboxyhemoglobin. This curve can also be produced in mathematical way (as control e.g.). In practice there are the following steps: blood collecting (from fingerpad), hematolysis, first photometric measurement, reduction, second measurement, then reading the result from the calibration curve. The method can be automated (as demonstrated with the \"Gilford System\") providing the same results as with the manual method. All steps of quality control are given in full detail; as is the preparation of the hemolysates with various percentages of Carboxyhemoglobin.", "contents": "[Rapid diagnosis of carbon monoxide poisoning and quantitative determination of CO-Hb in blood]. A quick diagnosis of carbon monoxide poisoning is a very difficult clinical problem. This paper describes a photometric method which permits a reliable diagnosis in a time of 7 to 10 minutes. Only 0.3 ml whole blood are necessary for the total procedure. Carboxyhemoglobin changes in absorption maximum slightly after reduction, whereas Oxyhemoglobin shows a significant change in extinction. Measurement of the hemolysate at the same wave length before and after alkaline reduction results in large differences in the extinctions (very large for Oxyhemoglobin and very small for Carboxyhemoglobin). Calibration curves are prepared by calibrating rest-extinction defined as: (formula: see text) to percent Carboxyhemoglobin. This curve can also be produced in mathematical way (as control e.g.). In practice there are the following steps: blood collecting (from fingerpad), hematolysis, first photometric measurement, reduction, second measurement, then reading the result from the calibration curve. The method can be automated (as demonstrated with the \"Gilford System\") providing the same results as with the manual method. All steps of quality control are given in full detail; as is the preparation of the hemolysates with various percentages of Carboxyhemoglobin."}
{"id": "PMID:478749", "title": "Effect of altered central and peripheral visual field stimulation on correct recognition and visual evoked response.", "content": "Hemispheric asymmetry was assessed using combined electrophysiological (visual evoked response) and behavioral (percentage-correct-recognition) techniques. Right-handed, right-eyed, male undergraduates who viewed tachistoscopically exposed CVCs and random shapes in both central and peripheral visual fields were scored for their ability to recognize the stimuli correctly. Latency and amplitude of visual evoked responses were compared with correct recognition. Central and peripheral stimuli produced significant results. Superiority of the left hemisphere for verbal stimulus processing was supported. Small but consistent positive peak latencies of visual evoked responses also indicated language specialization of the left lobe. Results were interpreted as supporting hemispheric functional asymmetry. Additional findings of \"cognitive masking\" and marked reduction in intersubject variance in postive peak latencies of visual evoked responses by a central stimulus occurring at approximately 300 msec were also obtained. Mechanisms of iconic image storage, neuropsychological attentional theories, and differential hemispheric structural organization were discussed in interpreting results.", "contents": "Effect of altered central and peripheral visual field stimulation on correct recognition and visual evoked response. Hemispheric asymmetry was assessed using combined electrophysiological (visual evoked response) and behavioral (percentage-correct-recognition) techniques. Right-handed, right-eyed, male undergraduates who viewed tachistoscopically exposed CVCs and random shapes in both central and peripheral visual fields were scored for their ability to recognize the stimuli correctly. Latency and amplitude of visual evoked responses were compared with correct recognition. Central and peripheral stimuli produced significant results. Superiority of the left hemisphere for verbal stimulus processing was supported. Small but consistent positive peak latencies of visual evoked responses also indicated language specialization of the left lobe. Results were interpreted as supporting hemispheric functional asymmetry. Additional findings of \"cognitive masking\" and marked reduction in intersubject variance in postive peak latencies of visual evoked responses by a central stimulus occurring at approximately 300 msec were also obtained. Mechanisms of iconic image storage, neuropsychological attentional theories, and differential hemispheric structural organization were discussed in interpreting results."}
{"id": "PMID:478750", "title": "The turnover of myelin proteins in adult rat brain.", "content": "The turnover of proteins of myelin of brain was followed after an intracerebral injections of [1-14C] leucine into adult rats and compared with the turnover of proteins of total brain homogenate, microsomal and supernatant fractions. The myelin proteins were separated into three major fractions, viz., the Wolfgram, proteolipid and basic proteins as fluorescamine derivatives by a new preparative sodium-dodecylsulfate-polyacrylamide slab gel electrophoresis method. The bands were visualized, without staining, by their fluoresence under UV light. The maximal incorporation into myelin proteins was about 11% of that in the microsomal proteins. The maximum amoount ofnto myelin proteins was about 11% of that in the microsomal proteins. The incorporation of the label in myelin proteins reached a moximum about six hr after the injection, while the microsomal and supernatant and myelin proteins declined at multiphasic rates with half-lives varying from three hours to several days. Individual myelin proteins also appeared to turn over at multiphasic rates, with half-lives of 5, 20 and 90-100 days for the Wolfgram proteins, 7 and 100-120 days for the proteolipid proteins and 5 and 80-110 days for the basic proteins.", "contents": "The turnover of myelin proteins in adult rat brain. The turnover of proteins of myelin of brain was followed after an intracerebral injections of [1-14C] leucine into adult rats and compared with the turnover of proteins of total brain homogenate, microsomal and supernatant fractions. The myelin proteins were separated into three major fractions, viz., the Wolfgram, proteolipid and basic proteins as fluorescamine derivatives by a new preparative sodium-dodecylsulfate-polyacrylamide slab gel electrophoresis method. The bands were visualized, without staining, by their fluoresence under UV light. The maximal incorporation into myelin proteins was about 11% of that in the microsomal proteins. The maximum amoount ofnto myelin proteins was about 11% of that in the microsomal proteins. The incorporation of the label in myelin proteins reached a moximum about six hr after the injection, while the microsomal and supernatant and myelin proteins declined at multiphasic rates with half-lives varying from three hours to several days. Individual myelin proteins also appeared to turn over at multiphasic rates, with half-lives of 5, 20 and 90-100 days for the Wolfgram proteins, 7 and 100-120 days for the proteolipid proteins and 5 and 80-110 days for the basic proteins."}
{"id": "PMID:478751", "title": "Relationship of age and education to performance on a standardized version of Luria's neuropsychological tests in different patient populations.", "content": "A.R. Luria, a Russian neuropsychologist, developed many qualitative bedside tests that have been effective in the diagnosis and localization of neurological disorders. Recently, a standardized and objectively-scored version of Luria's tests has been developed. Knowledge of the effects of patient age and education on neuro-psychological test performance has been found crucial in the neurodiagnostic decision-making process. The present study examined the effects of patient age(younger subjects between 20 and 40 years and older subjects between 50 and 70 years of age), education (grade school, high school, and post-high school), and diagnosis (normal, schizophrenic, brain damaged) on 14 standardized Luria measures. A weighted means analysis of variance found 11 significant age effects, 14 significant educational effects, and 14 significant effects diagnosis. One significant interaction was found between education and diagnosis, the results support the contention that with appropriate age and educational corrections, the standardized Luria battery would satisfy the need for a short, objectively scored, and diagnostically effective neuropsychological battery.", "contents": "Relationship of age and education to performance on a standardized version of Luria's neuropsychological tests in different patient populations. A.R. Luria, a Russian neuropsychologist, developed many qualitative bedside tests that have been effective in the diagnosis and localization of neurological disorders. Recently, a standardized and objectively-scored version of Luria's tests has been developed. Knowledge of the effects of patient age and education on neuro-psychological test performance has been found crucial in the neurodiagnostic decision-making process. The present study examined the effects of patient age(younger subjects between 20 and 40 years and older subjects between 50 and 70 years of age), education (grade school, high school, and post-high school), and diagnosis (normal, schizophrenic, brain damaged) on 14 standardized Luria measures. A weighted means analysis of variance found 11 significant age effects, 14 significant educational effects, and 14 significant effects diagnosis. One significant interaction was found between education and diagnosis, the results support the contention that with appropriate age and educational corrections, the standardized Luria battery would satisfy the need for a short, objectively scored, and diagnostically effective neuropsychological battery."}
{"id": "PMID:478753", "title": "Morphological differences in cerebral cortex and corpus callosum are genetically determined in two different strains of mice.", "content": "A morphological analysis of the cerebral cortex and corpus callosum was performed in two inbred strins of mice (SEC/1ReJ and C57BL/6J) different for a number of inborn and acquired behavioral patterns and for their ontogenic behavioral development. The data obtained indicate that in these two strains there are clear differences in the total thickness in the medial-anterior cortical region and temporal region, in the thickness and cell grey coefficient of the layers II-III, IV and V of some areas of parietal and frontal regions, and in the thickness of corpus callosum. These findings are discussed in terms of possible correlations between brain structure and behavior.", "contents": "Morphological differences in cerebral cortex and corpus callosum are genetically determined in two different strains of mice. A morphological analysis of the cerebral cortex and corpus callosum was performed in two inbred strins of mice (SEC/1ReJ and C57BL/6J) different for a number of inborn and acquired behavioral patterns and for their ontogenic behavioral development. The data obtained indicate that in these two strains there are clear differences in the total thickness in the medial-anterior cortical region and temporal region, in the thickness and cell grey coefficient of the layers II-III, IV and V of some areas of parietal and frontal regions, and in the thickness of corpus callosum. These findings are discussed in terms of possible correlations between brain structure and behavior."}
{"id": "PMID:478773", "title": "Erythrocyte electrolytes in recurrent affective disorders.", "content": "A modified method for determination of erythrocyte electrolytes is presented. The claims which have been made regarding the predictive value in periodic psychoses of erythrocyte electrolytes and 'lithium index' are questioned. Clinical experimental data of serial studies in patients receiving lithium suggests that theoretical predictions of distribution of lithium across erythrocyte membranes are not supported in practice and this lends support to the suggestion of active lithium transport in the red cell. A simple method is described of determining the attainment of 'stable state' in long term lithium treatment.", "contents": "Erythrocyte electrolytes in recurrent affective disorders. A modified method for determination of erythrocyte electrolytes is presented. The claims which have been made regarding the predictive value in periodic psychoses of erythrocyte electrolytes and 'lithium index' are questioned. Clinical experimental data of serial studies in patients receiving lithium suggests that theoretical predictions of distribution of lithium across erythrocyte membranes are not supported in practice and this lends support to the suggestion of active lithium transport in the red cell. A simple method is described of determining the attainment of 'stable state' in long term lithium treatment."}
{"id": "PMID:478774", "title": "Plasma magnesium in chronic schizophrenia. A preliminary report.", "content": "A group of 36 subjects, 20 chronic schizophrenic patients and 16 nonpsychotic mentally retarded patients were chosen at random from the chronic care wards of the Douglas Hospital to assess levels of magnesium, calcium and phosphorus. No significant differences were found between the two groups in calcium and phosphorus. However, the magnesium level was significantly lower (p less than 0.01) in the schizophrenic group than in the mentally retarded group. This difference reached a level of 0.001 significance when chronic female schizophrenic patients were compared to the female mentally retarded group. Hospitalization, diet and medication were excluded as possible etiological factors.", "contents": "Plasma magnesium in chronic schizophrenia. A preliminary report. A group of 36 subjects, 20 chronic schizophrenic patients and 16 nonpsychotic mentally retarded patients were chosen at random from the chronic care wards of the Douglas Hospital to assess levels of magnesium, calcium and phosphorus. No significant differences were found between the two groups in calcium and phosphorus. However, the magnesium level was significantly lower (p less than 0.01) in the schizophrenic group than in the mentally retarded group. This difference reached a level of 0.001 significance when chronic female schizophrenic patients were compared to the female mentally retarded group. Hospitalization, diet and medication were excluded as possible etiological factors."}
{"id": "PMID:478775", "title": "Turnover of mouse photoreceptor outer segments in constant light and darkness.", "content": "Pigmented mice differ from frogs in that lighting regime has only a small effect on rod outer segment turnover. During 8 days in constant light or darkness, disc addition measured as total displacement of a radioactive band in rod outer segments of mice which has received injections of tritiated amino acids was never modified by more than 7% compared to cyclic light controls. Disc shedding continued in each lighting regime, and as judged from outer segment dimensions, disc shedding approximately balanced disc addition.", "contents": "Turnover of mouse photoreceptor outer segments in constant light and darkness. Pigmented mice differ from frogs in that lighting regime has only a small effect on rod outer segment turnover. During 8 days in constant light or darkness, disc addition measured as total displacement of a radioactive band in rod outer segments of mice which has received injections of tritiated amino acids was never modified by more than 7% compared to cyclic light controls. Disc shedding continued in each lighting regime, and as judged from outer segment dimensions, disc shedding approximately balanced disc addition."}
{"id": "PMID:478776", "title": "Sustained release of BCNU for the treatment of intraocular malignancies in animal models.", "content": "Sustained release of 1,3-bis(2)chloroethyl)-1-nitrosourea (BCNU) via an episcleral implanted silicone device was used to treat Brown-Pearce epithelioma in the anterior chamber of rabbit eyes. One group of test eyes received BCNU dissolved in sesame oil; a second group received BCNU in pure ethanol. Control eyes received only the diluent, sesame oil or pure ethanol. The effectiveness of the various dosages and diluents was compared by clinical observation, by weight of the enucleated eyes, and by histopathologic examination. Sustained release of BCNU via an episcleral implanted silicone device delayed the growth of Brown-Pearce epithelioma in rabbit eyes of both test groups. The most effective action resulted from administration of BCNU in pure ethanol.", "contents": "Sustained release of BCNU for the treatment of intraocular malignancies in animal models. Sustained release of 1,3-bis(2)chloroethyl)-1-nitrosourea (BCNU) via an episcleral implanted silicone device was used to treat Brown-Pearce epithelioma in the anterior chamber of rabbit eyes. One group of test eyes received BCNU dissolved in sesame oil; a second group received BCNU in pure ethanol. Control eyes received only the diluent, sesame oil or pure ethanol. The effectiveness of the various dosages and diluents was compared by clinical observation, by weight of the enucleated eyes, and by histopathologic examination. Sustained release of BCNU via an episcleral implanted silicone device delayed the growth of Brown-Pearce epithelioma in rabbit eyes of both test groups. The most effective action resulted from administration of BCNU in pure ethanol."}
{"id": "PMID:478777", "title": "Blue-sensitive cones of the cat produce a rodlike electroretinogram.", "content": "Two cone mechanisms are identifiable in the strongly yellow light-adapted electroretinogram (ERG) of the arterially perfused cat eye. One has its maximum spectral sensitivity near 555 nm; the other has its maximum near 450 nm. The former cone system produces a much larger signal with characteristics of a typical cone or inhibitory ERG. The latter cone system produces a small, saturable signal (less than 5 microV) which resembles a rodlike or excitatory ERC. The results imply that the latter ERG is generated by blue-sensitive cones, which form a small fraction of the total cone population and share some physiological and perhaps anatomical properties of rods.", "contents": "Blue-sensitive cones of the cat produce a rodlike electroretinogram. Two cone mechanisms are identifiable in the strongly yellow light-adapted electroretinogram (ERG) of the arterially perfused cat eye. One has its maximum spectral sensitivity near 555 nm; the other has its maximum near 450 nm. The former cone system produces a much larger signal with characteristics of a typical cone or inhibitory ERG. The latter cone system produces a small, saturable signal (less than 5 microV) which resembles a rodlike or excitatory ERC. The results imply that the latter ERG is generated by blue-sensitive cones, which form a small fraction of the total cone population and share some physiological and perhaps anatomical properties of rods."}
{"id": "PMID:478778", "title": "Accommodative and fusional components of fixation disparity.", "content": "Traditional measurements of fixation disparity, like other binocular measurements, confound influences from both blur-driven and disparity-driven components. Measuring fixation disparity with accommodation open-loop eliminates accommodative interactions, and results show fixation disparity is reduced to about half the value observed normally for high levels of forced vergence. Thus fixation disparity curves are shaped by both accommodative and fusional vergence processes.", "contents": "Accommodative and fusional components of fixation disparity. Traditional measurements of fixation disparity, like other binocular measurements, confound influences from both blur-driven and disparity-driven components. Measuring fixation disparity with accommodation open-loop eliminates accommodative interactions, and results show fixation disparity is reduced to about half the value observed normally for high levels of forced vergence. Thus fixation disparity curves are shaped by both accommodative and fusional vergence processes."}
{"id": "PMID:478779", "title": "Secretory immune system of rabbit ocular adnexa.", "content": "A distinct system of immunity in a variety of animals is located subjacent to epithelial surfaces and is typified by the predominance of immunoglobulin A (IgA) and secretory component (SC) in various external secretions, including tears. The present study examined normal rabbit lacrimal gland, conjunctiva, and cornea for the presence of immunoglobulin and SC. IgA-staining plasma cells predominated within lacrimal gland and conjunctival stroma, and SC was found in the epithelial cells of both these tissues but not within corneal epithelium. These observations are consistent with findings for other secretory sites in both rabbits and humans and establish lacrimal gland and conjunctiva as integral parts of the rabbit secretory immune system.", "contents": "Secretory immune system of rabbit ocular adnexa. A distinct system of immunity in a variety of animals is located subjacent to epithelial surfaces and is typified by the predominance of immunoglobulin A (IgA) and secretory component (SC) in various external secretions, including tears. The present study examined normal rabbit lacrimal gland, conjunctiva, and cornea for the presence of immunoglobulin and SC. IgA-staining plasma cells predominated within lacrimal gland and conjunctival stroma, and SC was found in the epithelial cells of both these tissues but not within corneal epithelium. These observations are consistent with findings for other secretory sites in both rabbits and humans and establish lacrimal gland and conjunctiva as integral parts of the rabbit secretory immune system."}
{"id": "PMID:478781", "title": "The early appearance of disc shedding in the rat retina.", "content": "Postnatal development of the processes of rod outer segment shedding and phagocytosis was examined in 10- to 50-day-old rats. The number of phagosomes increased gradually from a minimallevel in the second postnatal week to that of the adult at 4 weeks after birth. No differences were observed in animals raised under two different light intensities i.e., relatively bright (45 to 50 ft-cd) and very dim (0.3 to 0.5 ft-cd). A definite circadian rhythm was observed first in 15-day-old animals, indicating that this is an important internal control mechanism that is synchronized with eye opening and the initiation of visual function.", "contents": "The early appearance of disc shedding in the rat retina. Postnatal development of the processes of rod outer segment shedding and phagocytosis was examined in 10- to 50-day-old rats. The number of phagosomes increased gradually from a minimallevel in the second postnatal week to that of the adult at 4 weeks after birth. No differences were observed in animals raised under two different light intensities i.e., relatively bright (45 to 50 ft-cd) and very dim (0.3 to 0.5 ft-cd). A definite circadian rhythm was observed first in 15-day-old animals, indicating that this is an important internal control mechanism that is synchronized with eye opening and the initiation of visual function."}
{"id": "PMID:478782", "title": "Passive radiotelemetry of intraocular pressure in vivo: calibration and validation of continual scleral guard-ring applanation transensors in the dog and rabbit.", "content": "Continual monitoring of intraocular pressure (IOP) by passive radiotelemetry, with a miniature scleral applanating device, mounted in a haptic contact lens (applanating transensor) is reported in the rabbit and dog. Several prototype transensors were calibrated in vivo in the rabbit and dog; a consistent linear output was obtained for each eye, although reproducibility of the slope between eyes over a period of time was less than ideal. Accuracy of determination of IOP was found to be better for the rabbit and will have to be improved before the instrument can be applied to the human. Multiple regression analysis of calibration studies in six rabbits established that the transensor output was not significantly affected by the small changes in body temperature and atmospheric pressure encountered during the study. Continual scleral tonometry with a standard Mackay-Marg tonometer showed that ocular rigidity has a significant effect on calibration curves, in contradistinction to standard Mackay-Marg corneal tonometry. A time-dependent decay in tonometer readings was more evident in canine eyes with low ocular rigidity. Since the transensor is also a guard-ring applanating device, calibration may be necessary for individual eyes and species.", "contents": "Passive radiotelemetry of intraocular pressure in vivo: calibration and validation of continual scleral guard-ring applanation transensors in the dog and rabbit. Continual monitoring of intraocular pressure (IOP) by passive radiotelemetry, with a miniature scleral applanating device, mounted in a haptic contact lens (applanating transensor) is reported in the rabbit and dog. Several prototype transensors were calibrated in vivo in the rabbit and dog; a consistent linear output was obtained for each eye, although reproducibility of the slope between eyes over a period of time was less than ideal. Accuracy of determination of IOP was found to be better for the rabbit and will have to be improved before the instrument can be applied to the human. Multiple regression analysis of calibration studies in six rabbits established that the transensor output was not significantly affected by the small changes in body temperature and atmospheric pressure encountered during the study. Continual scleral tonometry with a standard Mackay-Marg tonometer showed that ocular rigidity has a significant effect on calibration curves, in contradistinction to standard Mackay-Marg corneal tonometry. A time-dependent decay in tonometer readings was more evident in canine eyes with low ocular rigidity. Since the transensor is also a guard-ring applanating device, calibration may be necessary for individual eyes and species."}
{"id": "PMID:478783", "title": "Developmental ocular abnormalities in rats with X-ray--induced cataract mutation.", "content": "Prenatal and early postnatal devpelopment of eyes of Wistar rats with X-ray-induced cataract mutation was assessed histologically, to determine the events leading to cataract formation. A significant phenomenon appeared to be retinal folding, which occurred regularly at 14 to 15 days of gestation and might have pushed the lens against the cornea. A corneal reaction with the lens was indicated by corneal adhesions to the lens, seen frequently during and shortly after the period of retinal folding, and could have stimulated the epithelial hyperplasia that leads invariably to anterior polar cataract in these animals. Changes in the lens fiber cells, which could have been generated by the epithelial hyperplasia, included the sporadic detachment of newly developing fibers from the lens epithelium and the eventual swelling of the anterior ends of fibers still attached to the epithelium. The detached fibers became fusiform and developed postnatally into posterior, suture-associated vacuoles. Anterior uveitis was noted postnatally in some eyes and probably contributed to the subsequent development of the cataract. The results are discussed in the light of congenital anterior polar cataract formation reported in humans and other animals.", "contents": "Developmental ocular abnormalities in rats with X-ray--induced cataract mutation. Prenatal and early postnatal devpelopment of eyes of Wistar rats with X-ray-induced cataract mutation was assessed histologically, to determine the events leading to cataract formation. A significant phenomenon appeared to be retinal folding, which occurred regularly at 14 to 15 days of gestation and might have pushed the lens against the cornea. A corneal reaction with the lens was indicated by corneal adhesions to the lens, seen frequently during and shortly after the period of retinal folding, and could have stimulated the epithelial hyperplasia that leads invariably to anterior polar cataract in these animals. Changes in the lens fiber cells, which could have been generated by the epithelial hyperplasia, included the sporadic detachment of newly developing fibers from the lens epithelium and the eventual swelling of the anterior ends of fibers still attached to the epithelium. The detached fibers became fusiform and developed postnatally into posterior, suture-associated vacuoles. Anterior uveitis was noted postnatally in some eyes and probably contributed to the subsequent development of the cataract. The results are discussed in the light of congenital anterior polar cataract formation reported in humans and other animals."}
{"id": "PMID:478784", "title": "The dynamics of IgG in the cornea.", "content": "Fluoresceinated IgG was injected centrally into the rabbit's cornea and both its rate of loss from the tissue and its rate of spread in the stroma were estimated by objective fluorometry. A new technique for determining the rate of loss was established which required only the measurement of the maximum fluorescence in the tissue. The rate at which freshly formed IgG in the blood would arrive at the central regions of the cornea was estimated for the human eye. As penetration continues from the limbus and the protein is lost to the aqueous humor, a standing concentration gradient is set up with a minimum at the corneal center. Calculation suggests that this gradient should be small for IgG in the human eye.", "contents": "The dynamics of IgG in the cornea. Fluoresceinated IgG was injected centrally into the rabbit's cornea and both its rate of loss from the tissue and its rate of spread in the stroma were estimated by objective fluorometry. A new technique for determining the rate of loss was established which required only the measurement of the maximum fluorescence in the tissue. The rate at which freshly formed IgG in the blood would arrive at the central regions of the cornea was estimated for the human eye. As penetration continues from the limbus and the protein is lost to the aqueous humor, a standing concentration gradient is set up with a minimum at the corneal center. Calculation suggests that this gradient should be small for IgG in the human eye."}
{"id": "PMID:478785", "title": "The role of accommodative convergence at the limits of fusional vergence.", "content": "Two possible explanations are presented for the mechanism which limits the maximum fusional vergence response. An experimental paradigm is developed to differentiate between these alternatives. Experimental results indicate that the blur generally associated with strong fusional effort is due to convergence accommodation \"overdrive.\" Th important theoretical and clinical implications of this finding are presented.", "contents": "The role of accommodative convergence at the limits of fusional vergence. Two possible explanations are presented for the mechanism which limits the maximum fusional vergence response. An experimental paradigm is developed to differentiate between these alternatives. Experimental results indicate that the blur generally associated with strong fusional effort is due to convergence accommodation \"overdrive.\" Th important theoretical and clinical implications of this finding are presented."}
{"id": "PMID:478786", "title": "Improved electrode for electroretinography.", "content": "Corneal electrodes useful for clinical electroretinography require topical anesthesia, interfere with vision, can abrade, and are not well accepted by most children and many adults. A low mass conductive thread, corneal (DTL) electrode is described and comparatively tested against the Burian-Allen electrode. The DTL electrode was found to have few of the limitations of the hard contact lens electrode. Furthermore, the DTL electrode signal quality was comparable to that of the Burian-Allen electrode and provided less between-patient variability.", "contents": "Improved electrode for electroretinography. Corneal electrodes useful for clinical electroretinography require topical anesthesia, interfere with vision, can abrade, and are not well accepted by most children and many adults. A low mass conductive thread, corneal (DTL) electrode is described and comparatively tested against the Burian-Allen electrode. The DTL electrode was found to have few of the limitations of the hard contact lens electrode. Furthermore, the DTL electrode signal quality was comparable to that of the Burian-Allen electrode and provided less between-patient variability."}
{"id": "PMID:478791", "title": "Observer variation in interpreting radiographs of the pituitary fossa.", "content": "Observer variation in interpreting sellar radiographs in patients suspected or known to have a pituitary tumor has been examined. Two radiologists experienced in interpreting sellar radiographs examined independently, without clinical details, plain films and tomograms of the sella of 101 patients. In most, only minor changes were anticipated. Of the 93 female patients, 67 were under investigation for amenorrhea. Radiographs were examined four times, each radiologist examining each set twice. Appearances were classified as normal, doubtful or abnormal on each occasion. Overall intraobserver agreement was 76%--85%. Neither radiologist changes his opinion by more than one category, e.g. from normal to doubtful. Overall interobserver agreement was 63%--75%. Disagreement between observers concerning 11 (11%) of the patients resulted from differences of opinion about whether minor changes in sellar outline represented an abnormality or merely a normal variation. Kappa analysis suggested that much of the agreement may be ascribed to chance. Agreement rates resemble those for other clinical and radiological investigations.", "contents": "Observer variation in interpreting radiographs of the pituitary fossa. Observer variation in interpreting sellar radiographs in patients suspected or known to have a pituitary tumor has been examined. Two radiologists experienced in interpreting sellar radiographs examined independently, without clinical details, plain films and tomograms of the sella of 101 patients. In most, only minor changes were anticipated. Of the 93 female patients, 67 were under investigation for amenorrhea. Radiographs were examined four times, each radiologist examining each set twice. Appearances were classified as normal, doubtful or abnormal on each occasion. Overall intraobserver agreement was 76%--85%. Neither radiologist changes his opinion by more than one category, e.g. from normal to doubtful. Overall interobserver agreement was 63%--75%. Disagreement between observers concerning 11 (11%) of the patients resulted from differences of opinion about whether minor changes in sellar outline represented an abnormality or merely a normal variation. Kappa analysis suggested that much of the agreement may be ascribed to chance. Agreement rates resemble those for other clinical and radiological investigations."}
{"id": "PMID:478792", "title": "Bone mineral measurements of the central skeleton by in vivo neutron activation analysis for routine investigation os osteopenia.", "content": "In vivo neutron activation analysis has been used to measure bone mineral content in the central skeleton where osteoporotic fractures occur. To be of diagnostic value, the results must be normalized for body size. From data obtained from 74 healthy children and adults up to 55 years of age, we have found that the calcium in the central skeleton is approximately proportional to the cube of the subject's height. The correlation for the adults alone has an r value of 0.81. When data from both adults and children are used, r = 0.95. The validity of this cubic height relationship to the Ca concentration measurements has been further substantiated by studies on rats. The total femur calcium content of 110 rats from weanling to 25 weeks of age was proportional to the overall femur (length) 3.6 (or (length)2.6 per unit length) with r = 0.99. When the level of Ca content is related to data on normal subjects of the same body size (giving the Calcium Bone Index or CaBI) a good separation is obtained between normal volunteers and osteoporotic subjects. Volunteers who were 20 to 55 years of age had CABI 1.0 \"/- .12 (SD) while osteoporotics had CaBI 0.69 +/- .10 (SD). When the calcium content as determined by in vivo activation analysis is expressed as a CaBI, it provides a powerful tool for the diagnosis of osteopenia. We suggest that all bone measurements, including peripheral ones, be normalized for body size in order to increase their diagnostic value.", "contents": "Bone mineral measurements of the central skeleton by in vivo neutron activation analysis for routine investigation os osteopenia. In vivo neutron activation analysis has been used to measure bone mineral content in the central skeleton where osteoporotic fractures occur. To be of diagnostic value, the results must be normalized for body size. From data obtained from 74 healthy children and adults up to 55 years of age, we have found that the calcium in the central skeleton is approximately proportional to the cube of the subject's height. The correlation for the adults alone has an r value of 0.81. When data from both adults and children are used, r = 0.95. The validity of this cubic height relationship to the Ca concentration measurements has been further substantiated by studies on rats. The total femur calcium content of 110 rats from weanling to 25 weeks of age was proportional to the overall femur (length) 3.6 (or (length)2.6 per unit length) with r = 0.99. When the level of Ca content is related to data on normal subjects of the same body size (giving the Calcium Bone Index or CaBI) a good separation is obtained between normal volunteers and osteoporotic subjects. Volunteers who were 20 to 55 years of age had CABI 1.0 \"/- .12 (SD) while osteoporotics had CaBI 0.69 +/- .10 (SD). When the calcium content as determined by in vivo activation analysis is expressed as a CaBI, it provides a powerful tool for the diagnosis of osteopenia. We suggest that all bone measurements, including peripheral ones, be normalized for body size in order to increase their diagnostic value."}
{"id": "PMID:478790", "title": "Contrast gradient and the detection of lung nodules.", "content": "The effects of edge gradient and blur on the detection pulmonary nodules were studied, using a test series of 175 artificial nodules on 30 chest films. The nodules were of homogeneous texture, about 1 cm in diameter and had a mean contrast of .12. Six observers viewed the film set, scoring both location and confidence. The sensitivity or the probability of a true-positive response was linearly related to the blur and a modified ROC analysis resulted in a family of ROC curves with the index of detectability monotonically decreasing with increased blur.", "contents": "Contrast gradient and the detection of lung nodules. The effects of edge gradient and blur on the detection pulmonary nodules were studied, using a test series of 175 artificial nodules on 30 chest films. The nodules were of homogeneous texture, about 1 cm in diameter and had a mean contrast of .12. Six observers viewed the film set, scoring both location and confidence. The sensitivity or the probability of a true-positive response was linearly related to the blur and a modified ROC analysis resulted in a family of ROC curves with the index of detectability monotonically decreasing with increased blur."}
{"id": "PMID:478795", "title": "Transcatheter electrocoagulation: experimental evaluation of the anode.", "content": "Transcatheter electrocoagulation using direct current has been shown to be an effective method for vessel occlusion. To date, the stainless steel anode has not been entirely satisfactory. Thus, an evaluation of bipolar platinum electrodes and a comparison of monopolar platinum and stainless steel electrodes have been performed. Experimental results have shown that both monopolar and bipolar platinum electrodes caused perforation in most cases. At the present time, we have not found a satisfactory replacement for the unipolar stainless steel anode.", "contents": "Transcatheter electrocoagulation: experimental evaluation of the anode. Transcatheter electrocoagulation using direct current has been shown to be an effective method for vessel occlusion. To date, the stainless steel anode has not been entirely satisfactory. Thus, an evaluation of bipolar platinum electrodes and a comparison of monopolar platinum and stainless steel electrodes have been performed. Experimental results have shown that both monopolar and bipolar platinum electrodes caused perforation in most cases. At the present time, we have not found a satisfactory replacement for the unipolar stainless steel anode."}
{"id": "PMID:478794", "title": "A comparison of area-equivalent phases of systole and diastole as a method for evaluation of left ventricular movement.", "content": "A method of left ventricular motion evaluation is described that does not use the end-diastolic contour as a reference point. The systolic outline in the RAO view was divided into four equal time periods. The systolic outline at the end of each period was paired to the diastolic outline with approximately the same area. Index values were calculated in order to elucidate any existing asymmetry of movement between the anterior and diaphragmatic left ventricular wall. In a group of 14 patients with coronary artery disease without obvious local left ventricular contractility disturbances, pathological asymmetry index values were found in 10. In a group of 16 patients with local left ventricular contractility disturbances, abnormal asymmetry index values were found in eight. This index of assymmetry demonstrated pathological changes of left ventricular motion in spite of a normal appearance of local left ventricular contractility during visual evaluation.", "contents": "A comparison of area-equivalent phases of systole and diastole as a method for evaluation of left ventricular movement. A method of left ventricular motion evaluation is described that does not use the end-diastolic contour as a reference point. The systolic outline in the RAO view was divided into four equal time periods. The systolic outline at the end of each period was paired to the diastolic outline with approximately the same area. Index values were calculated in order to elucidate any existing asymmetry of movement between the anterior and diaphragmatic left ventricular wall. In a group of 14 patients with coronary artery disease without obvious local left ventricular contractility disturbances, pathological asymmetry index values were found in 10. In a group of 16 patients with local left ventricular contractility disturbances, abnormal asymmetry index values were found in eight. This index of assymmetry demonstrated pathological changes of left ventricular motion in spite of a normal appearance of local left ventricular contractility during visual evaluation."}
{"id": "PMID:478796", "title": "Rectal balloons: complications, causes, and recommendations.", "content": "Nine previously unreported rectal injuries caused by barium-enema examinations have been reviewed. In each case, the injury occurred in conjunction with inflation of a rectal balloon. Analysis of the clinical material suggested that certain mechanical properties of balloon catheter tips might transfer mechanical stress to the rectal wall and contribute to the observed injuries. Careful manometric evaluation of in vivo rectal balloons suggest that significant anatomic differences in patients may be clinically important. Further experimental bench studies revealed undesirable mechanical properties in many commercially available rectal balloon catheters. These mechanical problems include low compliance, asymmetrical inflation, strong lateral and anterior displacement of a firm catheter tip into the restraining wall, and self-obstruction of the balloon deflation outlet by the inflated baloon. Many of these problems were clinically confirmed by careful in vivo observations and by evidence collected from the nine cases of rectal injury. A series of practical prophylactic procedures are recommended.", "contents": "Rectal balloons: complications, causes, and recommendations. Nine previously unreported rectal injuries caused by barium-enema examinations have been reviewed. In each case, the injury occurred in conjunction with inflation of a rectal balloon. Analysis of the clinical material suggested that certain mechanical properties of balloon catheter tips might transfer mechanical stress to the rectal wall and contribute to the observed injuries. Careful manometric evaluation of in vivo rectal balloons suggest that significant anatomic differences in patients may be clinically important. Further experimental bench studies revealed undesirable mechanical properties in many commercially available rectal balloon catheters. These mechanical problems include low compliance, asymmetrical inflation, strong lateral and anterior displacement of a firm catheter tip into the restraining wall, and self-obstruction of the balloon deflation outlet by the inflated baloon. Many of these problems were clinically confirmed by careful in vivo observations and by evidence collected from the nine cases of rectal injury. A series of practical prophylactic procedures are recommended."}
{"id": "PMID:478793", "title": "Metrizamide, the ideal radiopaque filling material for detachable silicone balloon embolization.", "content": "Porcine visceral and extremity arteries were embolized with detachable silastic balloons; 10 balloons were filled with isoosmolar metrizamide, five were filled with 30% meglumine-sodium diatrizoate and five were filled with 3:1 mixtures of blood and 76% meglumine-sodium diatrizoate. Follow-up radiographs were obtained weekly for 2 weeks, at 4 weeks and at 5 months. At 3 months, arteriography was performed. All balloons occluded the vessel completely upon detachment. Balloons filled with metrizamide did not change their volume or radiopacity during the entire observation period and all remained inflated. The balloons filled with diatrizoate salts enlarged by osmotic growth during the first two weeks and there was a decrease in opacity. Thereafter, one balloon filled with 30% diatrizoate salt solution and one filled with blood-76% diatrizoate deflated. In the former, the embolized artery remained occluded, while in the latter, the vessel reopened and the balloon was found incorporated into the vessel wall at the site of detachment. It is concluded that isoosmolar metrizamide is an ideal filler for detachable silastic balloons since it does not lead to changes in volume or radiopacity and also guarantees long-term vascular occlusion.", "contents": "Metrizamide, the ideal radiopaque filling material for detachable silicone balloon embolization. Porcine visceral and extremity arteries were embolized with detachable silastic balloons; 10 balloons were filled with isoosmolar metrizamide, five were filled with 30% meglumine-sodium diatrizoate and five were filled with 3:1 mixtures of blood and 76% meglumine-sodium diatrizoate. Follow-up radiographs were obtained weekly for 2 weeks, at 4 weeks and at 5 months. At 3 months, arteriography was performed. All balloons occluded the vessel completely upon detachment. Balloons filled with metrizamide did not change their volume or radiopacity during the entire observation period and all remained inflated. The balloons filled with diatrizoate salts enlarged by osmotic growth during the first two weeks and there was a decrease in opacity. Thereafter, one balloon filled with 30% diatrizoate salt solution and one filled with blood-76% diatrizoate deflated. In the former, the embolized artery remained occluded, while in the latter, the vessel reopened and the balloon was found incorporated into the vessel wall at the site of detachment. It is concluded that isoosmolar metrizamide is an ideal filler for detachable silastic balloons since it does not lead to changes in volume or radiopacity and also guarantees long-term vascular occlusion."}
{"id": "PMID:478797", "title": "Computed tomography of splenic subcapsular hematomas: an experimental study in dogs.", "content": "Following baseline CT scans, splenic subcapsular hematomas were produced in dogs by direct splenic trauma. The CT scans of the spleen were obtained one to two hours before and after administration of IV contrast media and one, two, seven, 14 and 28 days following splenic trauma. Variations in the CT image of the hematomas was correlated with time and alterations in the chemical and histologic nature of the hematoma. Initially, the hematomas were isodense with the normal spleen and identified only following IV contrast administration. The initial number of hematomas identified by CT decreased to about half after seven to 14 days and then remained stable. Analysis of the hematomas revealed an initial increase in hemoglobin which was followed by a decrease. Dry weight increased, then decreased; lipid content decreased over time; and calcium content showed no appreciable change. The CT appearance of splenic subcapsular hematomas is dependent on the age of the hematoma. CT scans done soon after trauma should be performed following administration of intravenous contrast media, while less acute hematomas should be apparent without contrast media. Changes in attenuation values of hematomas appear due to a reduction in hemoglobin content and an increase in water content of the hematoma.", "contents": "Computed tomography of splenic subcapsular hematomas: an experimental study in dogs. Following baseline CT scans, splenic subcapsular hematomas were produced in dogs by direct splenic trauma. The CT scans of the spleen were obtained one to two hours before and after administration of IV contrast media and one, two, seven, 14 and 28 days following splenic trauma. Variations in the CT image of the hematomas was correlated with time and alterations in the chemical and histologic nature of the hematoma. Initially, the hematomas were isodense with the normal spleen and identified only following IV contrast administration. The initial number of hematomas identified by CT decreased to about half after seven to 14 days and then remained stable. Analysis of the hematomas revealed an initial increase in hemoglobin which was followed by a decrease. Dry weight increased, then decreased; lipid content decreased over time; and calcium content showed no appreciable change. The CT appearance of splenic subcapsular hematomas is dependent on the age of the hematoma. CT scans done soon after trauma should be performed following administration of intravenous contrast media, while less acute hematomas should be apparent without contrast media. Changes in attenuation values of hematomas appear due to a reduction in hemoglobin content and an increase in water content of the hematoma."}
{"id": "PMID:478798", "title": "Radiologic assessment of pulmonary arterial pressure and blood volume in chronic, diffuse, interstitial pulmonary diseases.", "content": "Chronic, diffuse, interstitial pulmonary diseases may cause an increase in mean pulmonary arterial pressure (PAP) and a decrease in pulmonary blood volume (PBV). We compared 12 cardiovascular and three parenchymal assessments on plain chest radiographs with values of PAP and PBV obtained during cardiac catheterization in 29 patients with such diseases (progressive systemic sclerosis 20, sarcoidosis six, miscellaneous three) and normal pulmonary venous pressures. PAP ranged from 10 to 40 torr (mean 19, SD +/- 7), PBV from 6.4 to 10.8% of total blood volume (mean 8.4, SD +/- 1.2). PBV was significantly related to eight radiologic variables. PAP was significantly related to the severity of parenchymal disease and size of the central pulmonary arteries, both of which were assessed radiologically. Diversion of blood flow to upper zones was significantly related to restriction of the pulmonary vascular bed, but was not necessarily a sign of increased PAP. In general, pulmonary hemodynamic abnormalities appeared proportional to the radiologic severity of parenchymal disease.", "contents": "Radiologic assessment of pulmonary arterial pressure and blood volume in chronic, diffuse, interstitial pulmonary diseases. Chronic, diffuse, interstitial pulmonary diseases may cause an increase in mean pulmonary arterial pressure (PAP) and a decrease in pulmonary blood volume (PBV). We compared 12 cardiovascular and three parenchymal assessments on plain chest radiographs with values of PAP and PBV obtained during cardiac catheterization in 29 patients with such diseases (progressive systemic sclerosis 20, sarcoidosis six, miscellaneous three) and normal pulmonary venous pressures. PAP ranged from 10 to 40 torr (mean 19, SD +/- 7), PBV from 6.4 to 10.8% of total blood volume (mean 8.4, SD +/- 1.2). PBV was significantly related to eight radiologic variables. PAP was significantly related to the severity of parenchymal disease and size of the central pulmonary arteries, both of which were assessed radiologically. Diversion of blood flow to upper zones was significantly related to restriction of the pulmonary vascular bed, but was not necessarily a sign of increased PAP. In general, pulmonary hemodynamic abnormalities appeared proportional to the radiologic severity of parenchymal disease."}
{"id": "PMID:478799", "title": "ROC analysis applied to the evaluation of medical imaging techniques.", "content": "Analysis in terms of the relative operating characteristic (ROC) has recently been applied to several studies of medical decision-making, primarily to decisions based on imaging techniques. This paper presents a brief description of the ROC, and shows how it provides a measure of diagnostic accuracy that is free of judgmental bias. The results of medical studies are reviewed, and the main questions of theory and method that have arisen in the medical context are identified. Certain of these questions are basic to any psychophysical test, in which case an attempt has been made to present the best available answers. Other questions are of special medical importance and relevant reports are reviewed along with a description of current efforts to provide answers.", "contents": "ROC analysis applied to the evaluation of medical imaging techniques. Analysis in terms of the relative operating characteristic (ROC) has recently been applied to several studies of medical decision-making, primarily to decisions based on imaging techniques. This paper presents a brief description of the ROC, and shows how it provides a measure of diagnostic accuracy that is free of judgmental bias. The results of medical studies are reviewed, and the main questions of theory and method that have arisen in the medical context are identified. Certain of these questions are basic to any psychophysical test, in which case an attempt has been made to present the best available answers. Other questions are of special medical importance and relevant reports are reviewed along with a description of current efforts to provide answers."}
{"id": "PMID:478800", "title": "Bone mineral analysis using single-energy computed tomography.", "content": "We present a new method for evaluating in vivo changes in bone mineralization in the peripheral skeleton, using computed tomography (CT). A set of bone mineralization indices are generated from numerous CT images of the patient's distal radius. The cross-sectional anatomy displayed by the CT scan allows for separate evaluation of the cortical and trabecular bone. Correction for possible drift of the CT number scale (Hounsfield scale) is achieved by scanning standard solutions of dipotassium hydrogen phosphate simultaneously with the forearm. Preliminary data indicate that this is a precise method for evaluating in vivo changes in bone mineralization.", "contents": "Bone mineral analysis using single-energy computed tomography. We present a new method for evaluating in vivo changes in bone mineralization in the peripheral skeleton, using computed tomography (CT). A set of bone mineralization indices are generated from numerous CT images of the patient's distal radius. The cross-sectional anatomy displayed by the CT scan allows for separate evaluation of the cortical and trabecular bone. Correction for possible drift of the CT number scale (Hounsfield scale) is achieved by scanning standard solutions of dipotassium hydrogen phosphate simultaneously with the forearm. Preliminary data indicate that this is a precise method for evaluating in vivo changes in bone mineralization."}
{"id": "PMID:478802", "title": "Inspiratory lung volume achieved on routine chest films.", "content": "Posteroanterior and lateral chest radiographs were obtained in 19 healthy subjects after routine instruction. Immediately afterwards, repeat films were obtained with the subjects vigorously coaxed to maximal inhalation. Total lung capacity was measured from both sets of radiographs. Normal subjects inhale to 95.5% +/- 3.8% (SD) of total lung capacity during routine chest radiography. Routine chest radiographs will provide useful total lung capacity measurements. Old chest radiographs, in addition to frequently being a mandatory part of the clinical evaluation of patients with chest problems, may provide the only antecedent information about lung function (total lung capacity).", "contents": "Inspiratory lung volume achieved on routine chest films. Posteroanterior and lateral chest radiographs were obtained in 19 healthy subjects after routine instruction. Immediately afterwards, repeat films were obtained with the subjects vigorously coaxed to maximal inhalation. Total lung capacity was measured from both sets of radiographs. Normal subjects inhale to 95.5% +/- 3.8% (SD) of total lung capacity during routine chest radiography. Routine chest radiographs will provide useful total lung capacity measurements. Old chest radiographs, in addition to frequently being a mandatory part of the clinical evaluation of patients with chest problems, may provide the only antecedent information about lung function (total lung capacity)."}
{"id": "PMID:478801", "title": "In vivo assessment of bone metabolism using the cortical striation index.", "content": "Metacarpal cortical striations were evaluated in 350 patients using fine-detail radiography with optical magnification. Compared to the control population, mean striation-indices were elevated in growing children, primary and secondary hyperparathyroidism, osteomalacia and reflex sympathetic dystrophy syndrome. This technique may provide a useful quantitative assessment of bone metabolism, allowing differentiation of normal from hypermetabolic states. Serial assessment of cortical striation may be useful in monitoring response to therapy.", "contents": "In vivo assessment of bone metabolism using the cortical striation index. Metacarpal cortical striations were evaluated in 350 patients using fine-detail radiography with optical magnification. Compared to the control population, mean striation-indices were elevated in growing children, primary and secondary hyperparathyroidism, osteomalacia and reflex sympathetic dystrophy syndrome. This technique may provide a useful quantitative assessment of bone metabolism, allowing differentiation of normal from hypermetabolic states. Serial assessment of cortical striation may be useful in monitoring response to therapy."}
{"id": "PMID:478804", "title": "Physical parameters affecting the visibility of small ventricular septal defects using two-dimensional echocardiography.", "content": "We report a study showing that two-dimensional echocardiography is capable of visualizing a ventricular septal defect as small as 3 mm. The physical parameters which determine the visibility of an aperture in the myocardium include the presence of anterior refractile tissue, the amount of ultrasound beam focusing and range over which this occurs, the angle of incidence of the ultrasound beam on the septum, and the number of lines of information per degree of sector sweep. These factors are illustrated by in vitro tests. The influence of operator controllable variables in two-dimensional sector scanning echocardiography is discussed.", "contents": "Physical parameters affecting the visibility of small ventricular septal defects using two-dimensional echocardiography. We report a study showing that two-dimensional echocardiography is capable of visualizing a ventricular septal defect as small as 3 mm. The physical parameters which determine the visibility of an aperture in the myocardium include the presence of anterior refractile tissue, the amount of ultrasound beam focusing and range over which this occurs, the angle of incidence of the ultrasound beam on the septum, and the number of lines of information per degree of sector sweep. These factors are illustrated by in vitro tests. The influence of operator controllable variables in two-dimensional sector scanning echocardiography is discussed."}
{"id": "PMID:478803", "title": "Sequential changes in the CT numbers of the normal canine kidney following intravenous contrast administration. I. The renal cortex.", "content": "Using a 30-second computed tomography (CT) scanner, the sequential changes in CT numbers of the kidney were determined in three healthy dogs after intravenous administration of sodium and meglumine diatrizoate and iothalamate. For all contrast media, the mean CT number of the cortex was greatest within 2 minutes of rapid intravenous administration. At doses comparable to those used clinically, a linear relationship was demonstrated between the amount of iodine administered (in mg/kg body weight) and the corrected peak mean CT number of the cortex, such that doubling the amount of iodine caused the corrected peak mean CT number of the cortex to approximately double. Following the peak, the mean CT number of the cortex gradually declined. For all contrast media, the corrected mean CT number to corrected peak mean CT number ratios at 5, 10 and 20 minutes were 58 +/- 3%, 39 +/- 3%, and 28 +/- 5% respectively. Our data suggest that rapid-sequence CT may be a valuable tool for evaluating the physiology of renal contrast media excretion.", "contents": "Sequential changes in the CT numbers of the normal canine kidney following intravenous contrast administration. I. The renal cortex. Using a 30-second computed tomography (CT) scanner, the sequential changes in CT numbers of the kidney were determined in three healthy dogs after intravenous administration of sodium and meglumine diatrizoate and iothalamate. For all contrast media, the mean CT number of the cortex was greatest within 2 minutes of rapid intravenous administration. At doses comparable to those used clinically, a linear relationship was demonstrated between the amount of iodine administered (in mg/kg body weight) and the corrected peak mean CT number of the cortex, such that doubling the amount of iodine caused the corrected peak mean CT number of the cortex to approximately double. Following the peak, the mean CT number of the cortex gradually declined. For all contrast media, the corrected mean CT number to corrected peak mean CT number ratios at 5, 10 and 20 minutes were 58 +/- 3%, 39 +/- 3%, and 28 +/- 5% respectively. Our data suggest that rapid-sequence CT may be a valuable tool for evaluating the physiology of renal contrast media excretion."}
{"id": "PMID:478805", "title": "Therapeutic embolization with detachable silicone balloons: long-term effects in swine.", "content": "Twenty-one embolizations were performed in 12 swine using a detachable silicone balloon-catheter system. In 11 embolizations, balloons were filled with 60% meglumine sodium diatrizoate (R-60); 30% meglumine sodium diatrizoate (R-30) was used in 10 embolizations. Serial radiographs were obtained to determine the duration of balloon inflation. Repeat angiography and autopsy examinations were performed three to six months following embolization. Eighty percent of balloons filled with R-30 were still inflated after 10 days, as opposed to 27% of the balloons filled with R-60. Balloons which remained inflated for more than 10 days caused permanent occlusion of the embolized arteries. The vessels were occluded precisely at the point at which the balloons were initially attached. Pathologically, the occluded vessels exhibited organized thrombi with minimal inflammation. Detachable balloons appear to be an effective, safe method of performing selective vascular occlusion in many circulatory systems. Careful control of the volume and osmolarity of the contrast medium used to inflate the balloons produces permanent arterial occlusion.", "contents": "Therapeutic embolization with detachable silicone balloons: long-term effects in swine. Twenty-one embolizations were performed in 12 swine using a detachable silicone balloon-catheter system. In 11 embolizations, balloons were filled with 60% meglumine sodium diatrizoate (R-60); 30% meglumine sodium diatrizoate (R-30) was used in 10 embolizations. Serial radiographs were obtained to determine the duration of balloon inflation. Repeat angiography and autopsy examinations were performed three to six months following embolization. Eighty percent of balloons filled with R-30 were still inflated after 10 days, as opposed to 27% of the balloons filled with R-60. Balloons which remained inflated for more than 10 days caused permanent occlusion of the embolized arteries. The vessels were occluded precisely at the point at which the balloons were initially attached. Pathologically, the occluded vessels exhibited organized thrombi with minimal inflammation. Detachable balloons appear to be an effective, safe method of performing selective vascular occlusion in many circulatory systems. Careful control of the volume and osmolarity of the contrast medium used to inflate the balloons produces permanent arterial occlusion."}
{"id": "PMID:478807", "title": "Protein binding to iothalamate ethyl ester.", "content": "A major problem in the development of a particulate hepatolienographic agent is the prevention of particle aggregation upon contact with blood. When iothalamate ethyl ester, a particulate suspension for potential use as an hepatolienographic agent, was mixed with human or animal plasmas, it resulted in moderate to severe flocculation. In a study conducted to define the binding of plasma proteins to the iothalamate ethyl ester particles, plasma was reversibly adsorbed to various particle preparations. Fractions obtained by incubation of the particle-plasma complexes in buffers of increased ionic strength and decreased pH were subjected to polyacrylamide disc gel electrophoresis. The fraction containing the specifically bound proteins produced three distinct bands corresponding to prealbumin, albumin, and fibrinogen. Since the particles flocculated only when mixed with plasma, it is postulated that the fibrinogen-particle interaction is a major factor leading to particle agglutination.", "contents": "Protein binding to iothalamate ethyl ester. A major problem in the development of a particulate hepatolienographic agent is the prevention of particle aggregation upon contact with blood. When iothalamate ethyl ester, a particulate suspension for potential use as an hepatolienographic agent, was mixed with human or animal plasmas, it resulted in moderate to severe flocculation. In a study conducted to define the binding of plasma proteins to the iothalamate ethyl ester particles, plasma was reversibly adsorbed to various particle preparations. Fractions obtained by incubation of the particle-plasma complexes in buffers of increased ionic strength and decreased pH were subjected to polyacrylamide disc gel electrophoresis. The fraction containing the specifically bound proteins produced three distinct bands corresponding to prealbumin, albumin, and fibrinogen. Since the particles flocculated only when mixed with plasma, it is postulated that the fibrinogen-particle interaction is a major factor leading to particle agglutination."}
{"id": "PMID:478806", "title": "Intracolonic pressures during barium-enema studies using the single- and double- contrast techniques.", "content": "Six dogs were examined by means of single- and double-contrast barium enemas, and colonic pressures and diameters were recorded at five intervals while the volume of the contrast medium was increased. Examinations were performed with and without administration of glucagon. Changes in colonic pressure and diameter were similar for the two techniques in 77% of the studies with and in 65% of the studies without administration of glucagon. Neither technique produced significantly different pressure or diameter changes with glucagon as compared to without it. Limitations in the experimental technique must be considered before extending results to human subjects.", "contents": "Intracolonic pressures during barium-enema studies using the single- and double- contrast techniques. Six dogs were examined by means of single- and double-contrast barium enemas, and colonic pressures and diameters were recorded at five intervals while the volume of the contrast medium was increased. Examinations were performed with and without administration of glucagon. Changes in colonic pressure and diameter were similar for the two techniques in 77% of the studies with and in 65% of the studies without administration of glucagon. Neither technique produced significantly different pressure or diameter changes with glucagon as compared to without it. Limitations in the experimental technique must be considered before extending results to human subjects."}
{"id": "PMID:478808", "title": "Development of a radiographic chest phantom with disease simulation.", "content": "An anthropomorphic chest phantom with realistic disease simulation is described with a review of previously available chest phantoms. The need for subjective image analysis is discussed and compared with the existing physics parameters as a means of evaluating image quality in chest radiology. An introduction to the use of such a radiographically realistic chest phantom with lung parenchymal detail and disease simulation is illustrated.", "contents": "Development of a radiographic chest phantom with disease simulation. An anthropomorphic chest phantom with realistic disease simulation is described with a review of previously available chest phantoms. The need for subjective image analysis is discussed and compared with the existing physics parameters as a means of evaluating image quality in chest radiology. An introduction to the use of such a radiographically realistic chest phantom with lung parenchymal detail and disease simulation is illustrated."}
{"id": "PMID:478816", "title": "Yersinia enterocolitica antibodies in thyroid disorders.", "content": "Yersinia enterocolitica agglutinating antibodies were present in 42% of 36 patients with thyroid disease and in none of 77 control subjects. Since the frequency of Yersinia infections in Israel is low, the association of thyroid diseases with Yersinia antibodies is of particular interest. The significance of this finding is discussed in view of some recent observations.", "contents": "Yersinia enterocolitica antibodies in thyroid disorders. Yersinia enterocolitica agglutinating antibodies were present in 42% of 36 patients with thyroid disease and in none of 77 control subjects. Since the frequency of Yersinia infections in Israel is low, the association of thyroid diseases with Yersinia antibodies is of particular interest. The significance of this finding is discussed in view of some recent observations."}
{"id": "PMID:478817", "title": "Leydig cell density and function and their relation to gonadotropins in infertile oligospermic men with varicocele.", "content": "Circulating gonadotropin and testosterone levels in infertile men with varicocele and sperm counts of less than 10(7)/ml were correlated with testicular Leydig cell density and in vitro testosterone synthesis. A significant correlation was found between Leydig cell density and both plasma testosterone levels and in vitro testosterone synthesis. Luteinizing hormone (LH) levels paralleled the in vitro testosterone synthesis. Although in the majority of patients, in vitro testosterone synthesis and Leydig cell density were low, plasma testosterone and LH levels were within the normal range. The only abnormal circulating hormonal level was high follicle-stimulating hormone in the most severely oligospermic patients. No difference were found between the right and left testes with regard to in vitro testosterone synthesis or Leydig cell density, and no correlation with patient age or site or degree of varicocele could be demonstrated. These findings suggest that Leydig cell dysfunction was the mechanism responsible for the oligospermia and infertility of these patients.", "contents": "Leydig cell density and function and their relation to gonadotropins in infertile oligospermic men with varicocele. Circulating gonadotropin and testosterone levels in infertile men with varicocele and sperm counts of less than 10(7)/ml were correlated with testicular Leydig cell density and in vitro testosterone synthesis. A significant correlation was found between Leydig cell density and both plasma testosterone levels and in vitro testosterone synthesis. Luteinizing hormone (LH) levels paralleled the in vitro testosterone synthesis. Although in the majority of patients, in vitro testosterone synthesis and Leydig cell density were low, plasma testosterone and LH levels were within the normal range. The only abnormal circulating hormonal level was high follicle-stimulating hormone in the most severely oligospermic patients. No difference were found between the right and left testes with regard to in vitro testosterone synthesis or Leydig cell density, and no correlation with patient age or site or degree of varicocele could be demonstrated. These findings suggest that Leydig cell dysfunction was the mechanism responsible for the oligospermia and infertility of these patients."}
{"id": "PMID:478818", "title": "In vitro sensitization of human lymphocytes against allogeneic cells by antigen-fed macrophages.", "content": "Primary in vitro sensitization of human lymphocytes against allogeneic tumor cells was induced by antigen-fed macrophages. Spleen or peripheral blood macrophages were fed with cell-free extracts of allogeneic tumor cells. Autologous lymphocytes that were sensitized by the macrophage culture for five days manifested cytotoxic activity against the corresponding target cells.", "contents": "In vitro sensitization of human lymphocytes against allogeneic cells by antigen-fed macrophages. Primary in vitro sensitization of human lymphocytes against allogeneic tumor cells was induced by antigen-fed macrophages. Spleen or peripheral blood macrophages were fed with cell-free extracts of allogeneic tumor cells. Autologous lymphocytes that were sensitized by the macrophage culture for five days manifested cytotoxic activity against the corresponding target cells."}
{"id": "PMID:478819", "title": "Acute intoxication with ajmaline.", "content": "A 57-year-old man ingested 1,000 mg of ajmaline with suicidal intent. He was unconscious, hypotensive and showed serious disturbances in cardiac conduction on admission. High serum and urine levels of ajmaline were found. Although only 4% of the ingested dose was excreted following forced diuresis, all evidence of toxicity disappeared within 21 h.", "contents": "Acute intoxication with ajmaline. A 57-year-old man ingested 1,000 mg of ajmaline with suicidal intent. He was unconscious, hypotensive and showed serious disturbances in cardiac conduction on admission. High serum and urine levels of ajmaline were found. Although only 4% of the ingested dose was excreted following forced diuresis, all evidence of toxicity disappeared within 21 h."}
{"id": "PMID:478820", "title": "Chronic bronchitis and bronchial asthma in a rural community in Israel: relation to socioenvironmental factors.", "content": "Prevalence rates of chronic bronchitis and asthma in a rural community in Israel in 1976 were 2.5 and 2.1%, respectively. The relationships with age, sex, country of origin, marital status, number of children, household crowding, education, occupation and cigarette-smoking habits were studied. A number of significant univariate associations were found. A multiple regression analysis showed a significant independent association between chronic bronchitis and age. Age alone accounted for 4.6% of the variance in chronic bronchitis. Five other variables were significantly associated with chronic bronchitis. Together, these accounted for an additional 1.3% of the variance in chronic bronchitis. The multivariate analysis showed that only one variable--the number of children--had an independent relationship with asthma. It accounted for 0.8% of the variance. All the variables together accounted for 1.3% of the variance observed in bronchial asthma.", "contents": "Chronic bronchitis and bronchial asthma in a rural community in Israel: relation to socioenvironmental factors. Prevalence rates of chronic bronchitis and asthma in a rural community in Israel in 1976 were 2.5 and 2.1%, respectively. The relationships with age, sex, country of origin, marital status, number of children, household crowding, education, occupation and cigarette-smoking habits were studied. A number of significant univariate associations were found. A multiple regression analysis showed a significant independent association between chronic bronchitis and age. Age alone accounted for 4.6% of the variance in chronic bronchitis. Five other variables were significantly associated with chronic bronchitis. Together, these accounted for an additional 1.3% of the variance in chronic bronchitis. The multivariate analysis showed that only one variable--the number of children--had an independent relationship with asthma. It accounted for 0.8% of the variance. All the variables together accounted for 1.3% of the variance observed in bronchial asthma."}
{"id": "PMID:478821", "title": "Comparison of radioimmunoassay and counterimmunoelectrophoresis for the detection of hepatitis B surface antigen carriers in a high-prevalence region.", "content": "Solid-phase radioimmunoassay (RIA) and counterimmunoelectrophoresis (CIE) were compared for the detection of hepatitis B surface antigen (HBsAg) carriers among blood donors in Jerusalem, which is in a region characterized by a high prevalence of HBsAg and a predominance of the ayw subtype. RIA detected 84% more HBsAg carriers than CIE, with the resultant exclusion of 1.8 times as many potentially infective donors. The results provide additional information concerning the specific increases in donor-carrier detection that are associated with the introduction of highly sensitive HBsAg tests in different geographic and epidemiologic circumstances.", "contents": "Comparison of radioimmunoassay and counterimmunoelectrophoresis for the detection of hepatitis B surface antigen carriers in a high-prevalence region. Solid-phase radioimmunoassay (RIA) and counterimmunoelectrophoresis (CIE) were compared for the detection of hepatitis B surface antigen (HBsAg) carriers among blood donors in Jerusalem, which is in a region characterized by a high prevalence of HBsAg and a predominance of the ayw subtype. RIA detected 84% more HBsAg carriers than CIE, with the resultant exclusion of 1.8 times as many potentially infective donors. The results provide additional information concerning the specific increases in donor-carrier detection that are associated with the introduction of highly sensitive HBsAg tests in different geographic and epidemiologic circumstances."}
{"id": "PMID:478822", "title": "Steady-state serum quinidine concentration: role in prophylactic therapy following acute myocardial infarction.", "content": "Steady-state serum quinidine concentrations were monitored in 24 patients with acute myocardial infarction who were on a 1,300-mg daily dosing regimen. Mean serum concentrations spanned the therapeutic range, from 2 to 6 microgram/ml in 21 patients. In no patient was the level of 7 microgram/ml exceeded. Mean levels were similar in patients with congestive heart failure [3.6 +/- 1.5 (SD) microgram/ml] as in those free of failure (3.2 +/- 1.3 microgram/ml), and did not vary with impairment of renal function. There was a significant correlation between mean individual serum quinidine concentrations and the rate-normalized QT interval prolongation (r = 0.54, P less than 0.01); however, variability of the response was high. Variability of the mean serum quinidine levels among individuals was 41%. Variability within individual patients was only 18%. In the individual patient receiving prophylactic oral quinidine therapy, monitoring serum quinidine levels appears to be an accurate, reproducible and pharmacologically significant guideline to therapy.", "contents": "Steady-state serum quinidine concentration: role in prophylactic therapy following acute myocardial infarction. Steady-state serum quinidine concentrations were monitored in 24 patients with acute myocardial infarction who were on a 1,300-mg daily dosing regimen. Mean serum concentrations spanned the therapeutic range, from 2 to 6 microgram/ml in 21 patients. In no patient was the level of 7 microgram/ml exceeded. Mean levels were similar in patients with congestive heart failure [3.6 +/- 1.5 (SD) microgram/ml] as in those free of failure (3.2 +/- 1.3 microgram/ml), and did not vary with impairment of renal function. There was a significant correlation between mean individual serum quinidine concentrations and the rate-normalized QT interval prolongation (r = 0.54, P less than 0.01); however, variability of the response was high. Variability of the mean serum quinidine levels among individuals was 41%. Variability within individual patients was only 18%. In the individual patient receiving prophylactic oral quinidine therapy, monitoring serum quinidine levels appears to be an accurate, reproducible and pharmacologically significant guideline to therapy."}
{"id": "PMID:478823", "title": "Effect of large doses of morphine on experimental myocardial ischemia in cats.", "content": "The effect of large doses of morphine (1 mg/kg, i.v.) on experimental myocardial ischemia was evaluated in anesthetized open-chest cats. Myocardial ischemia was produced by intermittent coronary artery occlusion and assessed by measuring ST-segment elevation in multiple epicardial leads. The results obtained in the group of animals given morphine were compared with values obtained in animals given saline (control group), propranolol or nitrous oxide (reference groups). Morphine injected before coronary occlusion produced a significant increase in ST-segment elevation after left anterior descending coronary artery occlusion, whereas no significant change was noted in animals receiving normal saline or nitrous oxide. As expected, in the animals that received propranolol there was a pronounced decrease in ST-segment elevation. Our data suggest that large doses of morphine may increase myocardial ischemia when administered before coronary occlusion in the anesthetized open-chest cat.", "contents": "Effect of large doses of morphine on experimental myocardial ischemia in cats. The effect of large doses of morphine (1 mg/kg, i.v.) on experimental myocardial ischemia was evaluated in anesthetized open-chest cats. Myocardial ischemia was produced by intermittent coronary artery occlusion and assessed by measuring ST-segment elevation in multiple epicardial leads. The results obtained in the group of animals given morphine were compared with values obtained in animals given saline (control group), propranolol or nitrous oxide (reference groups). Morphine injected before coronary occlusion produced a significant increase in ST-segment elevation after left anterior descending coronary artery occlusion, whereas no significant change was noted in animals receiving normal saline or nitrous oxide. As expected, in the animals that received propranolol there was a pronounced decrease in ST-segment elevation. Our data suggest that large doses of morphine may increase myocardial ischemia when administered before coronary occlusion in the anesthetized open-chest cat."}
{"id": "PMID:478825", "title": "Severe hypermotility during sleep in treatment of cataplexy with clomipramine.", "content": "A case of narcolepsy with marked cataplexy is described in detail. Clomipramine hydrochloride effectively controlled the cataplexy and partially alleviated the daytime sleep attacks. However, clomipramine treatment resulted in episodes of severe motor hyperactivity during sleep, which were most intense during rapid eye movement sleep.", "contents": "Severe hypermotility during sleep in treatment of cataplexy with clomipramine. A case of narcolepsy with marked cataplexy is described in detail. Clomipramine hydrochloride effectively controlled the cataplexy and partially alleviated the daytime sleep attacks. However, clomipramine treatment resulted in episodes of severe motor hyperactivity during sleep, which were most intense during rapid eye movement sleep."}
{"id": "PMID:478826", "title": "Dynamic morphology applied to human and animal leukemia cells.", "content": "Dynamic morphology, which describes the shape and surface architecture of fixed cells in terms related to their behavior in the living state, is based on the concurrent use of two methods: scanning electron microscopy and microcinematography. This combination has both advantages and disadvantages. In this study on leukemic cells, we were able to draw the following conclusions about the usefulness of dynamic morphology. It confirms that white blood cells do not flatten on a glass substrate; they stay spherical and are either round or polarized. Round cells of similar size, whatever their origin, cannot be classified by dynamic morphology. Polarized cells can be classified as blasts, promyelocytes, myelocytes, granulocytes and lymphocytes, although polarized blast cells of different origins cannot be differentiated. Dynamic morphology cannot classify the same cell type as benign or malignant.", "contents": "Dynamic morphology applied to human and animal leukemia cells. Dynamic morphology, which describes the shape and surface architecture of fixed cells in terms related to their behavior in the living state, is based on the concurrent use of two methods: scanning electron microscopy and microcinematography. This combination has both advantages and disadvantages. In this study on leukemic cells, we were able to draw the following conclusions about the usefulness of dynamic morphology. It confirms that white blood cells do not flatten on a glass substrate; they stay spherical and are either round or polarized. Round cells of similar size, whatever their origin, cannot be classified by dynamic morphology. Polarized cells can be classified as blasts, promyelocytes, myelocytes, granulocytes and lymphocytes, although polarized blast cells of different origins cannot be differentiated. Dynamic morphology cannot classify the same cell type as benign or malignant."}
{"id": "PMID:478827", "title": "Use of enzymatic and electron microscopy (freeze-etching) methods for studying ATP-dependent masking of erythrocyte membrane phospholipids.", "content": "Membrane phospholipids of ATP-depleted chicken, rat, and toad erythrocytes are more susceptible than fresh cells to hydrolysis by phospholipase C (Bacillus cereus), phospholipase A (bee venom), or the combination of these enzymes and sphingomyelinase. ATP depletion of chicken and rat erythrocytes greatly increased the membrane phospholipid fraction, which can be extracted by dry ether. Trinitrobenzene sulfonic acid attacked about 20% of the phosphatidylethanolamine of fresh chicken erythrocytes and 45% of ATP-depleted chicken erythrocytes. The intramembranous particles on the PF face of fresh chicken, rat, and toad erythrocytes are evenly distributed, while those in the same ATP-depleted erythrocytes are significantly clustered. The average distance between the intramembranous particles in the PF face of fresh chicken erythrocytes is about 13 nm, while in ATP-depleted erythrocytes it is about 30 nm. Studies with chicken erythrocytes revealed that ATP depletion is accompanied by dephosphorylation of certain membrane polypeptides. The correlation of dephosphorylation of membrane polypeptides, exposure of membrane phospholipids, and clustering of intramembranous particles is discussed.", "contents": "Use of enzymatic and electron microscopy (freeze-etching) methods for studying ATP-dependent masking of erythrocyte membrane phospholipids. Membrane phospholipids of ATP-depleted chicken, rat, and toad erythrocytes are more susceptible than fresh cells to hydrolysis by phospholipase C (Bacillus cereus), phospholipase A (bee venom), or the combination of these enzymes and sphingomyelinase. ATP depletion of chicken and rat erythrocytes greatly increased the membrane phospholipid fraction, which can be extracted by dry ether. Trinitrobenzene sulfonic acid attacked about 20% of the phosphatidylethanolamine of fresh chicken erythrocytes and 45% of ATP-depleted chicken erythrocytes. The intramembranous particles on the PF face of fresh chicken, rat, and toad erythrocytes are evenly distributed, while those in the same ATP-depleted erythrocytes are significantly clustered. The average distance between the intramembranous particles in the PF face of fresh chicken erythrocytes is about 13 nm, while in ATP-depleted erythrocytes it is about 30 nm. Studies with chicken erythrocytes revealed that ATP depletion is accompanied by dephosphorylation of certain membrane polypeptides. The correlation of dephosphorylation of membrane polypeptides, exposure of membrane phospholipids, and clustering of intramembranous particles is discussed."}
{"id": "PMID:478828", "title": "Investigation of crystallosis of the kidney by means of polarization, scanning, transmission and high-voltage electron microscopy.", "content": "This report deals with the investigation of renal crystallosis in the autopsy tissue of a fatal human case of ethylene glycol poisoning. A previously healthy 22-year-old man consumed about one liter of Prestone antifreeze, which contains 95% by weight of ethylene glycol. He died in coma 48 h later with clinical evidence of metabolic acidosis and renal shutdown. Postmortem histological examination showed abundant crystallosis of the renal tubules. Polarization microscopy demonstrated brilliant birefringence of the crystals. In addition to the tubular crystallosis, scanning electron microscopy showed crystalline particles within the glomeruli as well. Energy dispersive X-ray analysis of the crystals gave a strong calcium signal. The crystals were demonstrated by means of conventional and high-voltage electron microscopy. Electron diffraction gave several hexagonal patterns of calcium carbonate-calcite and a tetragonal pattern suggestive of calcium oxalate monohydrate. X-ray diffraction of the crystals separated from the kidney showed the presence of calcium carbonate and calcium oxalate monohydrate as the major constituents.", "contents": "Investigation of crystallosis of the kidney by means of polarization, scanning, transmission and high-voltage electron microscopy. This report deals with the investigation of renal crystallosis in the autopsy tissue of a fatal human case of ethylene glycol poisoning. A previously healthy 22-year-old man consumed about one liter of Prestone antifreeze, which contains 95% by weight of ethylene glycol. He died in coma 48 h later with clinical evidence of metabolic acidosis and renal shutdown. Postmortem histological examination showed abundant crystallosis of the renal tubules. Polarization microscopy demonstrated brilliant birefringence of the crystals. In addition to the tubular crystallosis, scanning electron microscopy showed crystalline particles within the glomeruli as well. Energy dispersive X-ray analysis of the crystals gave a strong calcium signal. The crystals were demonstrated by means of conventional and high-voltage electron microscopy. Electron diffraction gave several hexagonal patterns of calcium carbonate-calcite and a tetragonal pattern suggestive of calcium oxalate monohydrate. X-ray diffraction of the crystals separated from the kidney showed the presence of calcium carbonate and calcium oxalate monohydrate as the major constituents."}
{"id": "PMID:478829", "title": "Electron microscopic x-ray microanalysis in pathology: current status.", "content": "Electron microscopic X-ray microprobe analysis is very suited to pathological investigations. It may lead to a better understanding of the relationship between structure and function in disease processes. It may also help to detect localized abnormalities before the appearance of morphological changes. This methodology may thus contribute to earlier diagnoses and detection of the carrier state, and may aid in the study of reactions to injury and therapy.", "contents": "Electron microscopic x-ray microanalysis in pathology: current status. Electron microscopic X-ray microprobe analysis is very suited to pathological investigations. It may lead to a better understanding of the relationship between structure and function in disease processes. It may also help to detect localized abnormalities before the appearance of morphological changes. This methodology may thus contribute to earlier diagnoses and detection of the carrier state, and may aid in the study of reactions to injury and therapy."}
{"id": "PMID:478830", "title": "Diagnostic applications of scanning electron microscopy and microanalysis in pathology.", "content": "Microanalytical technology developed within the last decade provides important information in diagnostic pathology. Scanning electron microscopy, including backscattered electron imaging and energy dispersive X-ray analysis should become at least as valuable as polarized light microscopy, histochemistry and conventional transmission electron microscopy. Other as yet less available techniques such as the ion microprobe and laser Raman microprobe are also valuable. The pathologist should consider the use of microanalytic techniques in any disease process in which endogenous or exogenous materials may be present in the tissues, in the same manner in which one would perform stains for microorganisms. Cases are presented illustrating the tissue preparation and results of scanning electron microscopy and energy dispersive X-ray analysis in diagnosis.", "contents": "Diagnostic applications of scanning electron microscopy and microanalysis in pathology. Microanalytical technology developed within the last decade provides important information in diagnostic pathology. Scanning electron microscopy, including backscattered electron imaging and energy dispersive X-ray analysis should become at least as valuable as polarized light microscopy, histochemistry and conventional transmission electron microscopy. Other as yet less available techniques such as the ion microprobe and laser Raman microprobe are also valuable. The pathologist should consider the use of microanalytic techniques in any disease process in which endogenous or exogenous materials may be present in the tissues, in the same manner in which one would perform stains for microorganisms. Cases are presented illustrating the tissue preparation and results of scanning electron microscopy and energy dispersive X-ray analysis in diagnosis."}
{"id": "PMID:478855", "title": "[The dermoepidermal junction in skin diseases].", "content": "Dermatological conditions characterized by dermo-epidermal separation, basal lamina discontinuity, multiplication, and thickness variability, and/or irregularity of the subepidermal space are discribed. Pathological changes of the dermo-epidermal junction are considered to be destructive or reproductive. Both may appear in combination. Destructive processes may be relfected by dermo-epidermal separation. Epidermal cells and/or dermal connective tissue appear degenerated. Thickening of the lamina may occur by reactive hyperproduction or by precipitation of pathological materials. Reproductive processes of the junction originate in the epidermal cells and are reflected in multilayering of the basal lamina. Interruption of the lamina and irregularity of the subepidermal space often precede these phenomena.", "contents": "[The dermoepidermal junction in skin diseases]. Dermatological conditions characterized by dermo-epidermal separation, basal lamina discontinuity, multiplication, and thickness variability, and/or irregularity of the subepidermal space are discribed. Pathological changes of the dermo-epidermal junction are considered to be destructive or reproductive. Both may appear in combination. Destructive processes may be relfected by dermo-epidermal separation. Epidermal cells and/or dermal connective tissue appear degenerated. Thickening of the lamina may occur by reactive hyperproduction or by precipitation of pathological materials. Reproductive processes of the junction originate in the epidermal cells and are reflected in multilayering of the basal lamina. Interruption of the lamina and irregularity of the subepidermal space often precede these phenomena."}
{"id": "PMID:478856", "title": "[Infantile centrifugal abdominal lipodystrophy].", "content": "Lipodystrophia centrifugalis abdominalis infantilis is an atrophic skin disease characterized by a localized depression of the abdominal and/or chest wall of infants. The depression starts either from the groins and their surroundings or from the axillae and their surroundings, and later gradually enlarges centrifugally to involve the larger part of abdominal and/or chest wall. The depressed areas are usually surrounded by slight erythema and scaling. Regional lymphnodes are usually swollen. Histologically, the loss or decrease of the subcutaneous fat is noted in the well-depressed areas. On the other hand, in the surrounding erythematous areas marked to moderate amount of inflammatory infiltrate is present in the subcutaneous fat.", "contents": "[Infantile centrifugal abdominal lipodystrophy]. Lipodystrophia centrifugalis abdominalis infantilis is an atrophic skin disease characterized by a localized depression of the abdominal and/or chest wall of infants. The depression starts either from the groins and their surroundings or from the axillae and their surroundings, and later gradually enlarges centrifugally to involve the larger part of abdominal and/or chest wall. The depressed areas are usually surrounded by slight erythema and scaling. Regional lymphnodes are usually swollen. Histologically, the loss or decrease of the subcutaneous fat is noted in the well-depressed areas. On the other hand, in the surrounding erythematous areas marked to moderate amount of inflammatory infiltrate is present in the subcutaneous fat."}
{"id": "PMID:478857", "title": "[Immunotherapy of allergic disease. Studies on 460 patients].", "content": "The results and side effects of immunotherapy in atopic dermatitis, bronchial asthma and/or allergic rhinitis are evaluated in 460 patients. The findings are listed in terms of diagnosis, age, sex and preparations as well as duration and number of incorporated allergens of immunotherapy-vaccine. In 82% a good result was reported, while in 18% no improvement could be seen. The results increase gradually from atopic dermatitis to bronchial asthma and allergic rhinitis, although the differences fail to be significant statistically. Males respond better than females. However, in bronchial asthma only, the differences are significant (p less than 0.01). According to the preparations used (Bencard, Novo-Helisen, Allpyral), no differences could be seen. The percentage of side effects is higher than 50%, but is mainly restricted to local swelling, tiredness and itching. Again no significant differences could be seen between the vaccines used.", "contents": "[Immunotherapy of allergic disease. Studies on 460 patients]. The results and side effects of immunotherapy in atopic dermatitis, bronchial asthma and/or allergic rhinitis are evaluated in 460 patients. The findings are listed in terms of diagnosis, age, sex and preparations as well as duration and number of incorporated allergens of immunotherapy-vaccine. In 82% a good result was reported, while in 18% no improvement could be seen. The results increase gradually from atopic dermatitis to bronchial asthma and allergic rhinitis, although the differences fail to be significant statistically. Males respond better than females. However, in bronchial asthma only, the differences are significant (p less than 0.01). According to the preparations used (Bencard, Novo-Helisen, Allpyral), no differences could be seen. The percentage of side effects is higher than 50%, but is mainly restricted to local swelling, tiredness and itching. Again no significant differences could be seen between the vaccines used."}
{"id": "PMID:478858", "title": "[Motor-conduction velocity of cubital nerves in patients with leprosy reactions. Summary of a 13-year observation series during thalidomide therapy].", "content": "The motor conduction velocity of the ulnar nerves was examined repeatedly in 34 patients with lepromatous leprosy. In patients with active lesions the reduction in velocity paralleled clinical deterioration. In \"burned-out\" control patients, who had never suffered from leprosy reaction, the conduction velocity remained slow and unchanged over many years. Twenty-six of 34 patients suffering from leprosy reaction received Thalidomide treatment over a period of 6-13 years in order to suppress the reaction, with good effect. In none of these 26 patients neurotoxic disturbances were found.", "contents": "[Motor-conduction velocity of cubital nerves in patients with leprosy reactions. Summary of a 13-year observation series during thalidomide therapy]. The motor conduction velocity of the ulnar nerves was examined repeatedly in 34 patients with lepromatous leprosy. In patients with active lesions the reduction in velocity paralleled clinical deterioration. In \"burned-out\" control patients, who had never suffered from leprosy reaction, the conduction velocity remained slow and unchanged over many years. Twenty-six of 34 patients suffering from leprosy reaction received Thalidomide treatment over a period of 6-13 years in order to suppress the reaction, with good effect. In none of these 26 patients neurotoxic disturbances were found."}
{"id": "PMID:478859", "title": "[Disseminated piloleiomyomas and leiomyosarcomas of the skin].", "content": "Two cases of smooth muscle cell tumors of the skin are reported: a 39 year old female patient with disseminated piloleiomyomas on her left leg, and a 53 year old male patient with a leiomyosarcoma on his right shoulder. The clinical features and specially the typical histolocigal characteristics are shown.", "contents": "[Disseminated piloleiomyomas and leiomyosarcomas of the skin]. Two cases of smooth muscle cell tumors of the skin are reported: a 39 year old female patient with disseminated piloleiomyomas on her left leg, and a 53 year old male patient with a leiomyosarcoma on his right shoulder. The clinical features and specially the typical histolocigal characteristics are shown."}
{"id": "PMID:478860", "title": "[Paratibial symetric muscle hernias].", "content": "Two cases of uncommon tumor-like alterations on the lower legs are discussed to be related to peizongenic pedal papules and diagnosed as myoceles.", "contents": "[Paratibial symetric muscle hernias]. Two cases of uncommon tumor-like alterations on the lower legs are discussed to be related to peizongenic pedal papules and diagnosed as myoceles."}
{"id": "PMID:478861", "title": "[An unusual case of carpal tunnel syndrome].", "content": "A severe form of a carpal-tunnel-syndrome with motoric and sensoric deficits and trophic disturbances of the fingers II and III is presented, which reminds also of syringomyelia, erysipeloid or a chronic erysipelas.", "contents": "[An unusual case of carpal tunnel syndrome]. A severe form of a carpal-tunnel-syndrome with motoric and sensoric deficits and trophic disturbances of the fingers II and III is presented, which reminds also of syringomyelia, erysipeloid or a chronic erysipelas."}
{"id": "PMID:478975", "title": "Hemoglobin Dunn: alpha 6 (A4) aspartic acid replaced by asparagine.", "content": "Hemoglobin Dunn, alpha 6 (A4) Asp leads to Asn was found in a 39-year-old black woman and her daughter. The hematological data on the propositus were normal and there were no apparent clinical or pathological findings associated with this abnormal hemoglobin. The structural characterization of this variant is described.", "contents": "Hemoglobin Dunn: alpha 6 (A4) aspartic acid replaced by asparagine. Hemoglobin Dunn, alpha 6 (A4) Asp leads to Asn was found in a 39-year-old black woman and her daughter. The hematological data on the propositus were normal and there were no apparent clinical or pathological findings associated with this abnormal hemoglobin. The structural characterization of this variant is described."}
{"id": "PMID:478976", "title": "Hemoglobin Sunshine Seth - alpha 2 (94 (G1) Asp replaced by His) beta 2.", "content": "Hemoglobin Sunshine Seth in which a histidyl is substituted for an aspartyl residue at position 94 of the alpha chain was detected at birth in a Caucasian male infant during cord blood screening and is present also in the mother and a male sibling. Although the substitution is in the alpha 1 beta 2 contact, it is without obvious deleterious effect on the hematological parameters or the health of the affected individuals.", "contents": "Hemoglobin Sunshine Seth - alpha 2 (94 (G1) Asp replaced by His) beta 2. Hemoglobin Sunshine Seth in which a histidyl is substituted for an aspartyl residue at position 94 of the alpha chain was detected at birth in a Caucasian male infant during cord blood screening and is present also in the mother and a male sibling. Although the substitution is in the alpha 1 beta 2 contact, it is without obvious deleterious effect on the hematological parameters or the health of the affected individuals."}
{"id": "PMID:478977", "title": "Hemoglobin Suan-Dok (alpha 2 109 (G16) Leu replaced by Arg beta 2): an unstable variant associated with alpha-thalassemia.", "content": "An abnormal hemoglobin variant was detected in a seven-year old Thai girl. The clinical and hematological pictures were indistinguishable from hemoglobin H disease. Upon cellulose acetate electrophoresis at pH 8.5 an abnormal hemoglobin variant was found together with hemoglobins A and H. The variant moved slightly behind HbF and amounted to approximately 9%. Globin chain synthesis studies of peripheral blood reticulocytes incubated with tritiated leucine revealed that the total radioactivity ratio alpha-chain + variant alpha-chain/beta-chain was 0.59, while the ratio variant alpha-chain/beta-chain was 0.12. The variant hemoglobin was somewhat unstable under heat denaturation and in the isopropanol test. Structural studies by component isolation, globin chain separation, peptide mapping and aminoacid analysis of abnormal peptides showed that the leucine residue 109 of the alpha-chain was replaced by arginine. This hemoglobin mutant has not been described before and is named Hemoglobin Suan-Dok.", "contents": "Hemoglobin Suan-Dok (alpha 2 109 (G16) Leu replaced by Arg beta 2): an unstable variant associated with alpha-thalassemia. An abnormal hemoglobin variant was detected in a seven-year old Thai girl. The clinical and hematological pictures were indistinguishable from hemoglobin H disease. Upon cellulose acetate electrophoresis at pH 8.5 an abnormal hemoglobin variant was found together with hemoglobins A and H. The variant moved slightly behind HbF and amounted to approximately 9%. Globin chain synthesis studies of peripheral blood reticulocytes incubated with tritiated leucine revealed that the total radioactivity ratio alpha-chain + variant alpha-chain/beta-chain was 0.59, while the ratio variant alpha-chain/beta-chain was 0.12. The variant hemoglobin was somewhat unstable under heat denaturation and in the isopropanol test. Structural studies by component isolation, globin chain separation, peptide mapping and aminoacid analysis of abnormal peptides showed that the leucine residue 109 of the alpha-chain was replaced by arginine. This hemoglobin mutant has not been described before and is named Hemoglobin Suan-Dok."}
{"id": "PMID:478978", "title": "Globin synthesis in bone marrow cells of patients with sickle cell anemia and beta O-thalassemia: contamination of the beta-chain with non-globin proteins.", "content": "Chromatographic separation of labeled globin chains was performed in stroma-free hemolysates prepared from peripheral blood and bone marrow cells of 11 patients with beta O-thalassemia and 2 patients with sickle cell anemia. A small radioactivity peak, slightly preceding the beta-chain and more prominent in bone marrow cells, was often observed. This peak, which represents synthesis of non-globin proteins, did not exceed 5% of the radioactivity incorporated in the alpha-chain. It is concluded that contamination of the beta-chain with non-globin proteins undoubtedly occurs, but its extent is insufficient to explain the different synthetic ratios which have been repeatedly observed in peripheral blood and in bone marrow cells of patients with heterozygous beta-thalassemia.", "contents": "Globin synthesis in bone marrow cells of patients with sickle cell anemia and beta O-thalassemia: contamination of the beta-chain with non-globin proteins. Chromatographic separation of labeled globin chains was performed in stroma-free hemolysates prepared from peripheral blood and bone marrow cells of 11 patients with beta O-thalassemia and 2 patients with sickle cell anemia. A small radioactivity peak, slightly preceding the beta-chain and more prominent in bone marrow cells, was often observed. This peak, which represents synthesis of non-globin proteins, did not exceed 5% of the radioactivity incorporated in the alpha-chain. It is concluded that contamination of the beta-chain with non-globin proteins undoubtedly occurs, but its extent is insufficient to explain the different synthetic ratios which have been repeatedly observed in peripheral blood and in bone marrow cells of patients with heterozygous beta-thalassemia."}
{"id": "PMID:478979", "title": "Hemoglobin Lepore Washington and hemochromatosis in a Hungarian patient.", "content": "A Hungarian family with four heterozygotes for Hb Lepore Washington is described. One, a 43-year-old male, had high levels of serum iron, saturated iron-binding capacity, and ferritin, and normal levels of folic acid and vitamin B12. Liver biopsy showed slight cirrhosis and marked iron deposition in parenchymal cells and in cells of the reticuloendothelial system. Heavy iron deposition was also found in the bone marrow. The patient is not an alcoholic and has no disease that requires blood transfusion. The hemochromatosis thus seems to be of idiopathic nature.", "contents": "Hemoglobin Lepore Washington and hemochromatosis in a Hungarian patient. A Hungarian family with four heterozygotes for Hb Lepore Washington is described. One, a 43-year-old male, had high levels of serum iron, saturated iron-binding capacity, and ferritin, and normal levels of folic acid and vitamin B12. Liver biopsy showed slight cirrhosis and marked iron deposition in parenchymal cells and in cells of the reticuloendothelial system. Heavy iron deposition was also found in the bone marrow. The patient is not an alcoholic and has no disease that requires blood transfusion. The hemochromatosis thus seems to be of idiopathic nature."}
{"id": "PMID:478989", "title": "Development of neurons containing acetylcholinesterase and cholinacetyltransferase in dispersed cell culture of rat cerebellum.", "content": "Cells from one-day-old cerebellum were grown for up to 30 days in dispersed cell culture. The characteristic neurons (deep cerebellar, Golgi and Purkinje cells) maintained their properties. It was found histochemically that some of the large cells display strong AChE activities in the perikaryon and in some processes, while biochemically the specific activities of the marker enzymes of the acetylcholine system, AChE (EC 3.1.1.7) and ChAc (EC 2.3.1.6), were increased and unchanged, respectively. During cultivation, the number of AChE-positive neurons increased. It can be inferred from these studies that, besides the AChE-positive (cholinoceptive) cells, ChAc-active (cholinergic) neurons (possibly Golgi II. type cells and some neurons in the deep cerebellar nuclei) are present in the cerebellum of the rat.", "contents": "Development of neurons containing acetylcholinesterase and cholinacetyltransferase in dispersed cell culture of rat cerebellum. Cells from one-day-old cerebellum were grown for up to 30 days in dispersed cell culture. The characteristic neurons (deep cerebellar, Golgi and Purkinje cells) maintained their properties. It was found histochemically that some of the large cells display strong AChE activities in the perikaryon and in some processes, while biochemically the specific activities of the marker enzymes of the acetylcholine system, AChE (EC 3.1.1.7) and ChAc (EC 2.3.1.6), were increased and unchanged, respectively. During cultivation, the number of AChE-positive neurons increased. It can be inferred from these studies that, besides the AChE-positive (cholinoceptive) cells, ChAc-active (cholinergic) neurons (possibly Golgi II. type cells and some neurons in the deep cerebellar nuclei) are present in the cerebellum of the rat."}
{"id": "PMID:478991", "title": "Ultrastructural demonstration of dehydrogenases in rat cerebral cortex.", "content": "Techniques for the ultrastructural demonstration of dehydrogenases in cerebral cortex are described. The best fixation for good fine structural preservation and retention of LDH and NADH-diphorase was obtained by perfusion with a misture of formaldehyde and glutaraldehyde and for SDH by perfusion with formaldehyde. Comparison of incubation conditions showed that consistent results were obtained using enzyme markers NBT and DS-NBT for LDH and NADH-diaphorase: DS-NBT was more satisfactory than NBT and BSPT for SDH. Penetration of incubation media was improved by Triton X-100: DMSO and ultrasonic treatment were less effective. The techniques enabled the first electron cytochemical demonstration of dehydrogenases in different elements of prefixed cerebral cortex. Ultrastructural sites of enzyme activities were localized within cristae and inter-membrane spaces of mitochondria in nerve cell cytoplasm and its processes, oligodendrocytes and astrocytes. Authenticity of the ultrastructural sites was confirmed by four different control experiments.", "contents": "Ultrastructural demonstration of dehydrogenases in rat cerebral cortex. Techniques for the ultrastructural demonstration of dehydrogenases in cerebral cortex are described. The best fixation for good fine structural preservation and retention of LDH and NADH-diphorase was obtained by perfusion with a misture of formaldehyde and glutaraldehyde and for SDH by perfusion with formaldehyde. Comparison of incubation conditions showed that consistent results were obtained using enzyme markers NBT and DS-NBT for LDH and NADH-diaphorase: DS-NBT was more satisfactory than NBT and BSPT for SDH. Penetration of incubation media was improved by Triton X-100: DMSO and ultrasonic treatment were less effective. The techniques enabled the first electron cytochemical demonstration of dehydrogenases in different elements of prefixed cerebral cortex. Ultrastructural sites of enzyme activities were localized within cristae and inter-membrane spaces of mitochondria in nerve cell cytoplasm and its processes, oligodendrocytes and astrocytes. Authenticity of the ultrastructural sites was confirmed by four different control experiments."}
{"id": "PMID:478992", "title": "Further cytochemical studies on the perichromatin granules.", "content": "The perichromatin granules were studied in hepatocytes of experimental rats injected with cycloheximide because the increased number of these nuclear components after such treatment facilitated their cytochemical investigation. Most perichromatin granules were sensitive to the digestion with pepsin and ribonuclease. In contrast, small population of perichromatin granules was resistent to such digestion under conditions which remove known RNA containing components such as ribosomes, nucleolar RNP components and interchromatin granules. The size of these resistent perichromatin granules was reduced and they consisted of filaments the width of which was similar to that of filaments in the chromatin. Moreover, a small population of perichromatin granules was sensitive to the digestion with pepsin and deoxyribonuclease. The size of these granules was only slightly reduced. All these observations indicate that most perichromatin granules contain the RNA and some the DNA. A possibility also exists that the perichromatin granules might contain both RNA and DNA but in various proportions. In addition, partial digestion with pepsin followed by a complete digestion with ribonuclease and deoxyribonuclease removed perichromatin granules as well as other nucleoprotein structures. On the other hand, such digestion facilitated the visualization of the nuclear and cytoplasmic skeleton (matrix) in situ.", "contents": "Further cytochemical studies on the perichromatin granules. The perichromatin granules were studied in hepatocytes of experimental rats injected with cycloheximide because the increased number of these nuclear components after such treatment facilitated their cytochemical investigation. Most perichromatin granules were sensitive to the digestion with pepsin and ribonuclease. In contrast, small population of perichromatin granules was resistent to such digestion under conditions which remove known RNA containing components such as ribosomes, nucleolar RNP components and interchromatin granules. The size of these resistent perichromatin granules was reduced and they consisted of filaments the width of which was similar to that of filaments in the chromatin. Moreover, a small population of perichromatin granules was sensitive to the digestion with pepsin and deoxyribonuclease. The size of these granules was only slightly reduced. All these observations indicate that most perichromatin granules contain the RNA and some the DNA. A possibility also exists that the perichromatin granules might contain both RNA and DNA but in various proportions. In addition, partial digestion with pepsin followed by a complete digestion with ribonuclease and deoxyribonuclease removed perichromatin granules as well as other nucleoprotein structures. On the other hand, such digestion facilitated the visualization of the nuclear and cytoplasmic skeleton (matrix) in situ."}
{"id": "PMID:478993", "title": "Resolution of a gold latensification-elon ascorbic acid developer for Ilford L4 emulsion.", "content": "The electron-microscopical autoradiographical resolution of a gold latensification-elon ascorbic acid (GEA) developer for Ilford L4 emulsion was determined experimentally, using radioactive line sources of tritiated albumin (Heijnen and Geuze, 1977). For sections with a thickness of 62 nm (SD +/- 11), which were covered with a carbon layer about 5 nm thick and a slightly overlapping monolayer of L4 silver bromide crystals, the measured half-distance (HD) of resolution was 115 nm. This improvement in resolution, the high efficiency of the GEA developer for L4 emulsion (Wisse and Tates, 1968), and the excellent visibility of the cellular structures under the small silver grains, mean that the L4-GEA combination deserves preverence as a method for quantitative electron-microscopical autoradiography.", "contents": "Resolution of a gold latensification-elon ascorbic acid developer for Ilford L4 emulsion. The electron-microscopical autoradiographical resolution of a gold latensification-elon ascorbic acid (GEA) developer for Ilford L4 emulsion was determined experimentally, using radioactive line sources of tritiated albumin (Heijnen and Geuze, 1977). For sections with a thickness of 62 nm (SD +/- 11), which were covered with a carbon layer about 5 nm thick and a slightly overlapping monolayer of L4 silver bromide crystals, the measured half-distance (HD) of resolution was 115 nm. This improvement in resolution, the high efficiency of the GEA developer for L4 emulsion (Wisse and Tates, 1968), and the excellent visibility of the cellular structures under the small silver grains, mean that the L4-GEA combination deserves preverence as a method for quantitative electron-microscopical autoradiography."}
{"id": "PMID:478994", "title": "Folate metabolism in the superior cervical ganglion histochemical study.", "content": "By means of histochemical methods, folic acid, dihydrofolate reductase and NADH2-cytochrome-C-reductase were studied in the bovine superior cervical ganglion, in parallel with quantitative estimations of dihydrofolate reductase activity and in connection with the process of ageing. Various levels of folate metabolism were present in nerve cells and glial cells, as well as in pre or postganglionic nerves. In the process of ageing the activity of dihydrofolate reductase gradually decreased and the folic acid concentration in the nerve cells increased. Thus the enzyme --- substrate ratio appeared to favour the enzyme in young animals but the substrate in old animals.", "contents": "Folate metabolism in the superior cervical ganglion histochemical study. By means of histochemical methods, folic acid, dihydrofolate reductase and NADH2-cytochrome-C-reductase were studied in the bovine superior cervical ganglion, in parallel with quantitative estimations of dihydrofolate reductase activity and in connection with the process of ageing. Various levels of folate metabolism were present in nerve cells and glial cells, as well as in pre or postganglionic nerves. In the process of ageing the activity of dihydrofolate reductase gradually decreased and the folic acid concentration in the nerve cells increased. Thus the enzyme --- substrate ratio appeared to favour the enzyme in young animals but the substrate in old animals."}
{"id": "PMID:478995", "title": "The kinetics of the histochemical method for dihydrofolate dehydrogenase (dihydrofolate reductase).", "content": "The purpose of the present investigation is to report some histochemical and cytospectrophotometric observations providng more objective evidence for the specific activity of the histochemical method for dihydrofolate reductase activity (5.6.7.8-tetrahydrofolate: E.C. 1.5.1.3). Kinetic factors of the enzymic reaction, such as fixation, pH, coenzymes, substrate concentration, Michaelis constant, temperature, time of incubation, aerobiosis, and anaerobiosis, activators and inhibitors, were investigated.", "contents": "The kinetics of the histochemical method for dihydrofolate dehydrogenase (dihydrofolate reductase). The purpose of the present investigation is to report some histochemical and cytospectrophotometric observations providng more objective evidence for the specific activity of the histochemical method for dihydrofolate reductase activity (5.6.7.8-tetrahydrofolate: E.C. 1.5.1.3). Kinetic factors of the enzymic reaction, such as fixation, pH, coenzymes, substrate concentration, Michaelis constant, temperature, time of incubation, aerobiosis, and anaerobiosis, activators and inhibitors, were investigated."}
{"id": "PMID:478996", "title": "Dual wavelength scanning cytophotometry (Bicoscan).", "content": "A computer program has been developed for stage-scanning cytophotometry of double-stained microscopical specimens. The program permits the simultaneous measurement of absorbance values at two wavelengths in each measuring spot. To account for overlap in the absorbance spectra of the two stained endproducts, the program incorporates correction of the measured data to compensate for the contribution of each chromophore to the absorbance measured for the other. The program will compute the corrected local absorbance values at specified wavelengths for each chromophore at each measuring spot and integrate these values over the total object to give separate totals for each stain. It is also possible to have integrated the absorbance values of one chromophore for all those measuring spots where the local corrected absorbance value of the other chromophore exceeds a preset minimum value. When this other chromophore is a nuclear DNA stain, it is possible to obtain an approximate measure of the content of any compound in the nuclear area which can be stained with a chromophore having an absorbance spectrum different from the DNA stain. The validity of the program was investigated on model preparations consisting of two (differently) coloured films of which the absorbance values could be measured either individually or in combination, by partially overlaying one film on the other. The program's potential has been demonstrated by using the combination of either Naphthol Yellow S or dinitrofluorobenzene as protein stains with the Feulgen-pararosaniline(SO2) procedure for DNA in chicken erythrocytes and rat liver cells.", "contents": "Dual wavelength scanning cytophotometry (Bicoscan). A computer program has been developed for stage-scanning cytophotometry of double-stained microscopical specimens. The program permits the simultaneous measurement of absorbance values at two wavelengths in each measuring spot. To account for overlap in the absorbance spectra of the two stained endproducts, the program incorporates correction of the measured data to compensate for the contribution of each chromophore to the absorbance measured for the other. The program will compute the corrected local absorbance values at specified wavelengths for each chromophore at each measuring spot and integrate these values over the total object to give separate totals for each stain. It is also possible to have integrated the absorbance values of one chromophore for all those measuring spots where the local corrected absorbance value of the other chromophore exceeds a preset minimum value. When this other chromophore is a nuclear DNA stain, it is possible to obtain an approximate measure of the content of any compound in the nuclear area which can be stained with a chromophore having an absorbance spectrum different from the DNA stain. The validity of the program was investigated on model preparations consisting of two (differently) coloured films of which the absorbance values could be measured either individually or in combination, by partially overlaying one film on the other. The program's potential has been demonstrated by using the combination of either Naphthol Yellow S or dinitrofluorobenzene as protein stains with the Feulgen-pararosaniline(SO2) procedure for DNA in chicken erythrocytes and rat liver cells."}
{"id": "PMID:478997", "title": "A coupled peroxidatic oxidation technique for the histochemical localization of monoamine oxidase A and B and benzylamine oxidase.", "content": "A coupled peroxidatic oxidation technique is presented which employs benzylamine and tyramine as substrates and clorgyline, deprenyl, phenelzine and pargyline as specific inhibitors. Using this technique with frozen sections of human term placenta and rat liver, the histochemical localization of monocamine oxidase A and B and bnezylamine oxidase has been demonstrated.", "contents": "A coupled peroxidatic oxidation technique for the histochemical localization of monoamine oxidase A and B and benzylamine oxidase. A coupled peroxidatic oxidation technique is presented which employs benzylamine and tyramine as substrates and clorgyline, deprenyl, phenelzine and pargyline as specific inhibitors. Using this technique with frozen sections of human term placenta and rat liver, the histochemical localization of monocamine oxidase A and B and bnezylamine oxidase has been demonstrated."}
{"id": "PMID:478998", "title": "[Experimental heterograft tracheal transplants in animals. II (author's transl)].", "content": "In sequence with an earlier publication on the results of heterograft tracheal transplants in animals, changes in the Formol-Cialit preserved heterogenous tracheal grafts were studied by light microscopy, and by scanning and transmission electron microscopy. Two main queries arise. Where does the graft respiratory epithelium come from, and what happens to the grafted tracheal cartilage? We found that in the epithelium of graft the number of ciliated cells decreased and that there was ultrastructure evidence of cilial destruction, the goblet cells increased in number, there was a loss of strict polarity of the respiratory epithelium, subepithelial accumulations of cells of probable epithelial origin appeared, and there was a subepithelial accumulation of lymphocytes just beneath the basal lamina. Resorption and transformation of the grafted tracheal cartilage into connective tissue was also observed. Hence, the following conclusions may be made. (1) Small tracheal defects can be corrected by Formol-Cialit preserved heterologous grafts. (2) The mucosa of the host trachea produces a functioning respiratory epithelium in the graft. (3) Tracheal grafts should not exceed 2 x 3 cm in size otherwise a new stenosis from resorption and transformation of grafted cartilage into connective tissue will occur.", "contents": "[Experimental heterograft tracheal transplants in animals. II (author's transl)]. In sequence with an earlier publication on the results of heterograft tracheal transplants in animals, changes in the Formol-Cialit preserved heterogenous tracheal grafts were studied by light microscopy, and by scanning and transmission electron microscopy. Two main queries arise. Where does the graft respiratory epithelium come from, and what happens to the grafted tracheal cartilage? We found that in the epithelium of graft the number of ciliated cells decreased and that there was ultrastructure evidence of cilial destruction, the goblet cells increased in number, there was a loss of strict polarity of the respiratory epithelium, subepithelial accumulations of cells of probable epithelial origin appeared, and there was a subepithelial accumulation of lymphocytes just beneath the basal lamina. Resorption and transformation of the grafted tracheal cartilage into connective tissue was also observed. Hence, the following conclusions may be made. (1) Small tracheal defects can be corrected by Formol-Cialit preserved heterologous grafts. (2) The mucosa of the host trachea produces a functioning respiratory epithelium in the graft. (3) Tracheal grafts should not exceed 2 x 3 cm in size otherwise a new stenosis from resorption and transformation of grafted cartilage into connective tissue will occur."}
{"id": "PMID:478999", "title": "[Rupture of the round window membrane (author's transl)].", "content": "Labyrinthine fistula of the round window should be considered in the differential diagnosis of vertigo and sudden hearing loss occurring in patients, who are not only divers, during physical stress or exertion. Surgical exploration and closure of the fistulae are the only means of diagnosis and treatment of this condition. Since these fistulae may heal spontaneously in most instances early surgery is recommended after a short interval of conservative therapy. Five patients, of whom 4 underwent surgery and one was seen for an expert opinion are presented.", "contents": "[Rupture of the round window membrane (author's transl)]. Labyrinthine fistula of the round window should be considered in the differential diagnosis of vertigo and sudden hearing loss occurring in patients, who are not only divers, during physical stress or exertion. Surgical exploration and closure of the fistulae are the only means of diagnosis and treatment of this condition. Since these fistulae may heal spontaneously in most instances early surgery is recommended after a short interval of conservative therapy. Five patients, of whom 4 underwent surgery and one was seen for an expert opinion are presented."}
{"id": "PMID:479000", "title": "[A review of spontaneous cerebrospinal fluid rhinorrhoea (author's transl)].", "content": "Thirty-two patients described in the literature between 1947 and 1977, together with a personal one, with spontaneous cerebrospinal fluid rhinorrhoea are presented. These 32 patients are assessed and the different aspects of their problems discussed. There is a striking female to male preponderance of 5:1.", "contents": "[A review of spontaneous cerebrospinal fluid rhinorrhoea (author's transl)]. Thirty-two patients described in the literature between 1947 and 1977, together with a personal one, with spontaneous cerebrospinal fluid rhinorrhoea are presented. These 32 patients are assessed and the different aspects of their problems discussed. There is a striking female to male preponderance of 5:1."}
{"id": "PMID:479001", "title": "[Complications of transtympanic eustacian tube insufflation (author's transl)].", "content": "Serous otitis media is sometimes treated by Eustacian tube catheterization or by insufflation of the middle ear via a myringotomy. Both techniques can cause death as has been described in the literature. Two patients, in whom following middle ear insufflation via a myringotomy air was found intracranially are presented. In one the finding on skull X-ray was coincidental and no symptoms occurred, whereas in the other a paresis of the right foot that lasted for 30 min occurred from the time of the middle ear insufflation for his serous otitis media.", "contents": "[Complications of transtympanic eustacian tube insufflation (author's transl)]. Serous otitis media is sometimes treated by Eustacian tube catheterization or by insufflation of the middle ear via a myringotomy. Both techniques can cause death as has been described in the literature. Two patients, in whom following middle ear insufflation via a myringotomy air was found intracranially are presented. In one the finding on skull X-ray was coincidental and no symptoms occurred, whereas in the other a paresis of the right foot that lasted for 30 min occurred from the time of the middle ear insufflation for his serous otitis media."}
{"id": "PMID:479002", "title": "[Stereoscopic photography in plastic surgery (author's transl)].", "content": "A major problem in reconstructive and plastic surgery of the face is the assessment of exact distances between certain points of reference across facial contours. Incorrect estimation of these variables produces unsatisfactory postoperative anatomical results. Therefore a method, which permits exact measurements using stereoscopic photography of models, was tested. On the models quantitative studies of the effects of surgical techniques were made. Facial asymmetry and various phases of facial animation can be assessed and documented, and the progress of expanding tumours can likewise be followed.", "contents": "[Stereoscopic photography in plastic surgery (author's transl)]. A major problem in reconstructive and plastic surgery of the face is the assessment of exact distances between certain points of reference across facial contours. Incorrect estimation of these variables produces unsatisfactory postoperative anatomical results. Therefore a method, which permits exact measurements using stereoscopic photography of models, was tested. On the models quantitative studies of the effects of surgical techniques were made. Facial asymmetry and various phases of facial animation can be assessed and documented, and the progress of expanding tumours can likewise be followed."}
{"id": "PMID:479004", "title": "Surface modification of intraocular lenses to reduce corneal endothelial damage.", "content": "Two methods of surface modification of PMMA, permanent and temporary, were investigated for use on intraocular lenses to reduce corneal endothelial damage due to corneal-IOL touch. Monomer grafting using gamma irradiation was found to produce permanently hydrophilic surfaces. Temporary surface modifications developed and tested were peelable and dissolvable coatings. Test samples were touched to freshly excised rabbit corneas to evaluate the effectiveness of the various surface modifications in reducing endothelial cell damage. This touch testing was performed using static testing in which the test sample was touched to the cornea without movement and dynamic testing in which there was relative movement between the cornea and the test sample. While unmodified PMMA did significant cell damage in both static and dynamic tests perpamently modified (hydrophilic grafted) surfaces were found to perform well in the static, but not in the dynamic tests. Dissolvable coatings performed well in both tests, even with very thin coatings. There was little differentiation between the various water-soluble coatings tested. Due to its characteristics and rate of dissolution, polyvinyl alcohol appears to be an optimum material for coating IOLs. A series of in vitro and in vivo tests performed to assess its safety indicate that PVA is nontoxic and safe in animal eyes.", "contents": "Surface modification of intraocular lenses to reduce corneal endothelial damage. Two methods of surface modification of PMMA, permanent and temporary, were investigated for use on intraocular lenses to reduce corneal endothelial damage due to corneal-IOL touch. Monomer grafting using gamma irradiation was found to produce permanently hydrophilic surfaces. Temporary surface modifications developed and tested were peelable and dissolvable coatings. Test samples were touched to freshly excised rabbit corneas to evaluate the effectiveness of the various surface modifications in reducing endothelial cell damage. This touch testing was performed using static testing in which the test sample was touched to the cornea without movement and dynamic testing in which there was relative movement between the cornea and the test sample. While unmodified PMMA did significant cell damage in both static and dynamic tests perpamently modified (hydrophilic grafted) surfaces were found to perform well in the static, but not in the dynamic tests. Dissolvable coatings performed well in both tests, even with very thin coatings. There was little differentiation between the various water-soluble coatings tested. Due to its characteristics and rate of dissolution, polyvinyl alcohol appears to be an optimum material for coating IOLs. A series of in vitro and in vivo tests performed to assess its safety indicate that PVA is nontoxic and safe in animal eyes."}
{"id": "PMID:479006", "title": "Indomethacin as a means of preventing cystoid macular edema following intracapsular cataract extraction.", "content": "It has been theorized that prostaglandins E1 and E2 may be responsible for the vascular leakage leading to cystoid macular edema following cataract extraction. Indomethacin is a known inhibitor of prostaglandin synthesis. A prospective, double-blind study to evaluate the effect of oral indomethacin on four and eight week cases of postoperative CME following intracapsular cataract extraction as determined by fluorescein angiography was carried out on 42 patients. Twenty patients received 25 mg of indomethacin three times a day for three days preoperatively and three weeks postoperatively. Twenty-two patients received a placebo on an identical schedule. Four (20%) patients in the indomethacin group and five (22.7%) patients in the placebo group had positive angiograms for CME. No contributory factor resulting in CME was found.", "contents": "Indomethacin as a means of preventing cystoid macular edema following intracapsular cataract extraction. It has been theorized that prostaglandins E1 and E2 may be responsible for the vascular leakage leading to cystoid macular edema following cataract extraction. Indomethacin is a known inhibitor of prostaglandin synthesis. A prospective, double-blind study to evaluate the effect of oral indomethacin on four and eight week cases of postoperative CME following intracapsular cataract extraction as determined by fluorescein angiography was carried out on 42 patients. Twenty patients received 25 mg of indomethacin three times a day for three days preoperatively and three weeks postoperatively. Twenty-two patients received a placebo on an identical schedule. Four (20%) patients in the indomethacin group and five (22.7%) patients in the placebo group had positive angiograms for CME. No contributory factor resulting in CME was found."}
{"id": "PMID:479007", "title": "Primary vs. secondary insertion of anterior chamber lenses.", "content": "In this prospective study, patients with cataracts were divided into two groups. In Group I, intracapsular cataract extraction with anterior chamber lens insertion was performed as a one-step procedure. Patients in Group II underwent intracapsular cataract extraction followed by secondary insertion of an anterior chamber lens two to three months later. The higher incidence of CME seen with the secondary procedure seems to indicate that primary rather than secondary insertion of anterior chamber lenses is advisable.", "contents": "Primary vs. secondary insertion of anterior chamber lenses. In this prospective study, patients with cataracts were divided into two groups. In Group I, intracapsular cataract extraction with anterior chamber lens insertion was performed as a one-step procedure. Patients in Group II underwent intracapsular cataract extraction followed by secondary insertion of an anterior chamber lens two to three months later. The higher incidence of CME seen with the secondary procedure seems to indicate that primary rather than secondary insertion of anterior chamber lenses is advisable."}
{"id": "PMID:479070", "title": "Volumetric method for determing traces of organic chlorine in lipids.", "content": "The official AOAC method for determining chlorine in cake flour lipids has been modified to determine chlorine in lipids of animal carcasses cooled with chlorinated water. The sensitivity of the method has been improved 1000-fold to attain a detection limit of 2 ppm Cl in lipids. Recovery of the fine silver chloride precipitate was improved through centrifugation rather than filtration. The official method also did not take into consideration that when the silver chloride precipitate is exposed to light, it is converted to free chlorine and silver. Recovery of organic chlorine ranged from 84.6% at 4.1 ppm to 100.1% at 100 ppm. A number of samples of commercially chlorinated beef, pork, and chicken and laboratory-chlorinated chicken were analyzed by this method. In all cases where the level of chlorination was sufficient to result in a chlorine level in lipids in excess of 2 ppm, the chlorine content of the carcass lipids was measurable.", "contents": "Volumetric method for determing traces of organic chlorine in lipids. The official AOAC method for determining chlorine in cake flour lipids has been modified to determine chlorine in lipids of animal carcasses cooled with chlorinated water. The sensitivity of the method has been improved 1000-fold to attain a detection limit of 2 ppm Cl in lipids. Recovery of the fine silver chloride precipitate was improved through centrifugation rather than filtration. The official method also did not take into consideration that when the silver chloride precipitate is exposed to light, it is converted to free chlorine and silver. Recovery of organic chlorine ranged from 84.6% at 4.1 ppm to 100.1% at 100 ppm. A number of samples of commercially chlorinated beef, pork, and chicken and laboratory-chlorinated chicken were analyzed by this method. In all cases where the level of chlorination was sufficient to result in a chlorine level in lipids in excess of 2 ppm, the chlorine content of the carcass lipids was measurable."}
{"id": "PMID:479071", "title": "Detection of Salmonella in onion and garlic powders: collaborative study.", "content": "The relative efficiency of trypticase soy broth with added 0.5% K2SO3 and lactose broth as pre-enrichment media for recovering Salmonella from onion powder and garlic powder was collaboratively studied. For each spice, 13 collaborators each received 5 duplicate samples; 4 of the 5 replicate samples were inoculated with 1 of 4 levels of S. thompson ranging, at initiation of analysis, from greater than 3 to 93 organisms/g onion powder and greater than 3 to 43 organisms/g garlic powder. Salmonella growth was inhibited in each of these spices as evidenced by a rapid decline of most probable number values in samples determined immediately after and 7 days following inoculation. Collaborative results of cultural analyses demonstrated superiority of the modified tripticase soy broth for recovering Salmonella in each of the 2 spices. The improved method of detecting Salmonella in onion and garlic powders has been adopted as official first action.", "contents": "Detection of Salmonella in onion and garlic powders: collaborative study. The relative efficiency of trypticase soy broth with added 0.5% K2SO3 and lactose broth as pre-enrichment media for recovering Salmonella from onion powder and garlic powder was collaboratively studied. For each spice, 13 collaborators each received 5 duplicate samples; 4 of the 5 replicate samples were inoculated with 1 of 4 levels of S. thompson ranging, at initiation of analysis, from greater than 3 to 93 organisms/g onion powder and greater than 3 to 43 organisms/g garlic powder. Salmonella growth was inhibited in each of these spices as evidenced by a rapid decline of most probable number values in samples determined immediately after and 7 days following inoculation. Collaborative results of cultural analyses demonstrated superiority of the modified tripticase soy broth for recovering Salmonella in each of the 2 spices. The improved method of detecting Salmonella in onion and garlic powders has been adopted as official first action."}
{"id": "PMID:479072", "title": "Spectrophotometric method for hydroxymethylfurfural in honey.", "content": "A new method is described for hydroxymethylfurfural (HMF) in honey; accuracy and precision are improved over the most used optical and chemical methods. With a clarified honey solution containing 0.1% sodium bisulfite as reference and a similar solution without bisulfite as sample, a difference spectrum is obtained which represents only the HMF in the sample, without the interfering absorption of the honey. The average recovery was 97.5% for 24 additions to honey of 0.8--40 mg HMF/100 g. Forty honey samples ranging from 0 to 40 mg/100 g were analyzed by 3 methods with the following average results: Winkler optical method, 7.25; Winkler chemical method, 4.83; and new bisulfite method, 5.05 mg HMF/100 g honey. Values by the latter 2 methods did not differ at the P = 0.05 significance level.", "contents": "Spectrophotometric method for hydroxymethylfurfural in honey. A new method is described for hydroxymethylfurfural (HMF) in honey; accuracy and precision are improved over the most used optical and chemical methods. With a clarified honey solution containing 0.1% sodium bisulfite as reference and a similar solution without bisulfite as sample, a difference spectrum is obtained which represents only the HMF in the sample, without the interfering absorption of the honey. The average recovery was 97.5% for 24 additions to honey of 0.8--40 mg HMF/100 g. Forty honey samples ranging from 0 to 40 mg/100 g were analyzed by 3 methods with the following average results: Winkler optical method, 7.25; Winkler chemical method, 4.83; and new bisulfite method, 5.05 mg HMF/100 g honey. Values by the latter 2 methods did not differ at the P = 0.05 significance level."}
{"id": "PMID:479073", "title": "Methods for determinging carbohydrates, hydroxymethylfurfural, and proline in honey: collaborative study.", "content": "A modification of the official selective absorption method for honey carbohydrates, 31.124--31.133, was studied collaboratively; the determinations of sucrose, total monosaccharides, disaccharides, and higher sugars by this procedure were satisfactory and were adopted by the AOAC. High pressure liquid chromatography of glucose, fructose, and sucrose in honey showed better precision than the modified official method and gave concordant results; it was also adopted. Two methods for hydroxymethylfurfural do not qualify. A method for proline was also adopted.", "contents": "Methods for determinging carbohydrates, hydroxymethylfurfural, and proline in honey: collaborative study. A modification of the official selective absorption method for honey carbohydrates, 31.124--31.133, was studied collaboratively; the determinations of sucrose, total monosaccharides, disaccharides, and higher sugars by this procedure were satisfactory and were adopted by the AOAC. High pressure liquid chromatography of glucose, fructose, and sucrose in honey showed better precision than the modified official method and gave concordant results; it was also adopted. Two methods for hydroxymethylfurfural do not qualify. A method for proline was also adopted."}
{"id": "PMID:479074", "title": "Spectrophotometric determination of tolbutamide in tablets.", "content": "Two spectrophotometric methods, Glenn's method of orthogonal function and the basic dye method, are described for determing tolbutamide in tablets without interference from the tablet excipients. In Glenn's method, the absorbance of tolbutamide in 95% ethanol is measured in the vicinity of 250--270 nm at 4 nm intervals and the p2 coefficient is calculated. The coefficient is linearly related to concentration within a range of 0.1--0.4 mg/mL. Tolbutamide gives a complex of a ratio 1:1 with basic dye Brilliant Cresyl Blue (BCB) or Safranin T (ST). The complex is easily extracted with chloroform. The absorbance of the chloroform extract is measured against either a blank or reference experiment. The latter is obtained by using a specific concentration of tolbutamide: 0.4 mg/mL in tolbutamide-BCB or 0.024 mg/mL in tolbutamide-ST. The ST complex method is more sensitive compared with the other methods. When the t-test is applied, the results of the proposed methods are more accurate than those of the traditional ultraviolet spectrophotometric method.", "contents": "Spectrophotometric determination of tolbutamide in tablets. Two spectrophotometric methods, Glenn's method of orthogonal function and the basic dye method, are described for determing tolbutamide in tablets without interference from the tablet excipients. In Glenn's method, the absorbance of tolbutamide in 95% ethanol is measured in the vicinity of 250--270 nm at 4 nm intervals and the p2 coefficient is calculated. The coefficient is linearly related to concentration within a range of 0.1--0.4 mg/mL. Tolbutamide gives a complex of a ratio 1:1 with basic dye Brilliant Cresyl Blue (BCB) or Safranin T (ST). The complex is easily extracted with chloroform. The absorbance of the chloroform extract is measured against either a blank or reference experiment. The latter is obtained by using a specific concentration of tolbutamide: 0.4 mg/mL in tolbutamide-BCB or 0.024 mg/mL in tolbutamide-ST. The ST complex method is more sensitive compared with the other methods. When the t-test is applied, the results of the proposed methods are more accurate than those of the traditional ultraviolet spectrophotometric method."}
{"id": "PMID:479075", "title": "Spectrophotometric determination of acetaminophen in tablets, sirups, and suppositories.", "content": "A differential spectrophotometric method and Glenn's method of orthogonal function are described for the assay of acetaminophen in tablets, sirups, and suppositories. Interference from excipients in the formulations is thereby avoided. Accuracy of the analyses is greater with the proposed methods than with the British Pharmacopoeia method.", "contents": "Spectrophotometric determination of acetaminophen in tablets, sirups, and suppositories. A differential spectrophotometric method and Glenn's method of orthogonal function are described for the assay of acetaminophen in tablets, sirups, and suppositories. Interference from excipients in the formulations is thereby avoided. Accuracy of the analyses is greater with the proposed methods than with the British Pharmacopoeia method."}
{"id": "PMID:479076", "title": "Semiautomated method for the analysis of formulations of chlorpheniramine maleate and brompheniramine maleate.", "content": "The determination of chlorpheniramine maleate and brompheniramine maleate in tablets, capsules, injections, and elixirs has been automated. The active ingredient is dissolved in dilute HCl. The dilute acid solution is sampled, made basic with dilute NaOH, and extracted with isooctane. The isooctane phase is resampled and the drug is re-extracted into dilute HCl. The absorbance of the acidic aqueous layer is monitored at 265 nm. The method is an automated version of the general USP XIX assay for salts of organic nitrogenous bases. The results from the semiautomated procedure agree well with the USP XIX and NF XIV official methods. Recoveries were 100% from an authentic tablet material. The system is linear from 0 to 300% of declared potency. The procedure is free from common excipient and dye interferences. Precision data are included for both the automated and official methods.", "contents": "Semiautomated method for the analysis of formulations of chlorpheniramine maleate and brompheniramine maleate. The determination of chlorpheniramine maleate and brompheniramine maleate in tablets, capsules, injections, and elixirs has been automated. The active ingredient is dissolved in dilute HCl. The dilute acid solution is sampled, made basic with dilute NaOH, and extracted with isooctane. The isooctane phase is resampled and the drug is re-extracted into dilute HCl. The absorbance of the acidic aqueous layer is monitored at 265 nm. The method is an automated version of the general USP XIX assay for salts of organic nitrogenous bases. The results from the semiautomated procedure agree well with the USP XIX and NF XIV official methods. Recoveries were 100% from an authentic tablet material. The system is linear from 0 to 300% of declared potency. The procedure is free from common excipient and dye interferences. Precision data are included for both the automated and official methods."}
{"id": "PMID:479077", "title": "DC polarography of cephradine and its application to capsules.", "content": "A polarographic method has been developed for the quantitative analysis of cephradine and its dosage forms. Direct determinations on capsules are carried out; excipients and coloring matter do not interfere in the determination. The electroactive product is formed by acidic hydrolysis with 5.0N HCl and heating at 80 degrees C for 60 min. Two polarographic waves are obtained: I = -0.46 V and II = -0.78 v vs. SCE. Both reduction waves are diffusion controlled. Wave I is preferred for analytical purposes. The precise chemical identity of the electroactive product has not been determined, but UV spectral data and the TLC Rf value are reported. A linear relation is established for levels of cephradine between 10(-2) and 10(-5)M in 5.0N HCl.", "contents": "DC polarography of cephradine and its application to capsules. A polarographic method has been developed for the quantitative analysis of cephradine and its dosage forms. Direct determinations on capsules are carried out; excipients and coloring matter do not interfere in the determination. The electroactive product is formed by acidic hydrolysis with 5.0N HCl and heating at 80 degrees C for 60 min. Two polarographic waves are obtained: I = -0.46 V and II = -0.78 v vs. SCE. Both reduction waves are diffusion controlled. Wave I is preferred for analytical purposes. The precise chemical identity of the electroactive product has not been determined, but UV spectral data and the TLC Rf value are reported. A linear relation is established for levels of cephradine between 10(-2) and 10(-5)M in 5.0N HCl."}
{"id": "PMID:479078", "title": "Gas-liquid chromatographic quantitation of phencyclidine.HCl in powders: collaborative study.", "content": "Six laboratories collaboratively studied a method for the quantitative gas-liquid chromatographic (GLC) determination of phencyclidine.HCl in powders. The phencyclidine.HCl and other water-soluble compounds are dissolved in dilute HCl. A portion of the aqueous solution is made weakly basic with K2HPO4, and the organic-soluble compounds are extracted in CHCl3 for the GLC determination of the phencyclidine.HCl. Eicosane (n-C20) is incorporated in the extracting CHCl3 as an internal standard. The samples collaboratively studied included samples of known phencyclidine.HCl concentration and one sample of unknown purity. Recoveries ranged from 92.1 to 104% and per cent standard deviations from 1.05 to 3.39. The method was adopted as official first action.", "contents": "Gas-liquid chromatographic quantitation of phencyclidine.HCl in powders: collaborative study. Six laboratories collaboratively studied a method for the quantitative gas-liquid chromatographic (GLC) determination of phencyclidine.HCl in powders. The phencyclidine.HCl and other water-soluble compounds are dissolved in dilute HCl. A portion of the aqueous solution is made weakly basic with K2HPO4, and the organic-soluble compounds are extracted in CHCl3 for the GLC determination of the phencyclidine.HCl. Eicosane (n-C20) is incorporated in the extracting CHCl3 as an internal standard. The samples collaboratively studied included samples of known phencyclidine.HCl concentration and one sample of unknown purity. Recoveries ranged from 92.1 to 104% and per cent standard deviations from 1.05 to 3.39. The method was adopted as official first action."}
{"id": "PMID:479079", "title": "Laser fluorometric determination of aflatoxin B1 in corn.", "content": "A 2-step chromatographic separation, using both thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC), in conjunction with the high sensitivity of laser fluorometry permits extension of the detection limits of aflatoxin contamination in corn to 0.1 ppb (microgram/kg) with a 26% root mean square variation. Aflatoxin B1 is extracted from corn with water-methanol and cleaned up by TLC. The recovery of aflatoxin from the TLC plates was linear from 10 to 1000 pg. Aflatoxin B1 is converted to the more highly fluorescent B2A derivative by treatment with 1N HCl. Experiments with aflatoxin B1 standard establish a constant conversion to B2A over approximately 3 orders of magnitude in B1 concentration. An extract of the B2A aflatoxin derivative is injected onto a reverse phase HPLC column. A flowing droplet of eluant is irradiated by an amplitude-modulated 325 nm He-Cd ion laser beam, and fluorescence from the droplet is detected by a lock-in amplifier in phase with the laser modulation. Several chromatograms are presented that demonstrate the capability of this procedure for removing interfering components in the corn extract.", "contents": "Laser fluorometric determination of aflatoxin B1 in corn. A 2-step chromatographic separation, using both thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC), in conjunction with the high sensitivity of laser fluorometry permits extension of the detection limits of aflatoxin contamination in corn to 0.1 ppb (microgram/kg) with a 26% root mean square variation. Aflatoxin B1 is extracted from corn with water-methanol and cleaned up by TLC. The recovery of aflatoxin from the TLC plates was linear from 10 to 1000 pg. Aflatoxin B1 is converted to the more highly fluorescent B2A derivative by treatment with 1N HCl. Experiments with aflatoxin B1 standard establish a constant conversion to B2A over approximately 3 orders of magnitude in B1 concentration. An extract of the B2A aflatoxin derivative is injected onto a reverse phase HPLC column. A flowing droplet of eluant is irradiated by an amplitude-modulated 325 nm He-Cd ion laser beam, and fluorescence from the droplet is detected by a lock-in amplifier in phase with the laser modulation. Several chromatograms are presented that demonstrate the capability of this procedure for removing interfering components in the corn extract."}
{"id": "PMID:479080", "title": "Thin layer chromatographic determination of citrinin.", "content": "A thin layer chromatographic (TLC) method is described for the determination of citrinin in feeds. Citrinin is extracted from feed with methanol and water, the mixture is made alkaline with 10% sodium carbonate, and the aqueous solution is filtered and extracted with chloroform to remove most of the interfering materials. The aqueous layer is acidified with 2N HCl and extracted with chloroform. The chloroform extract is concentrated and spotted on a thin layer chromatographic (TLC) plate which is developed in chloroform-acetone-ethanol-water (60 + 40 + 10 + 1). The citrinin is viewed under ultraviolet light after TLC. Either visual or fluorodensitometric quantitation is used. Recoveries of citrinin from various feed samples spiked at levels of 2.0--5 micrograms/g were 75--92%. The proposed method can detect 0.5 micrograms/g feed, including corn, silage, ready mixed feeds, and feed pellets.", "contents": "Thin layer chromatographic determination of citrinin. A thin layer chromatographic (TLC) method is described for the determination of citrinin in feeds. Citrinin is extracted from feed with methanol and water, the mixture is made alkaline with 10% sodium carbonate, and the aqueous solution is filtered and extracted with chloroform to remove most of the interfering materials. The aqueous layer is acidified with 2N HCl and extracted with chloroform. The chloroform extract is concentrated and spotted on a thin layer chromatographic (TLC) plate which is developed in chloroform-acetone-ethanol-water (60 + 40 + 10 + 1). The citrinin is viewed under ultraviolet light after TLC. Either visual or fluorodensitometric quantitation is used. Recoveries of citrinin from various feed samples spiked at levels of 2.0--5 micrograms/g were 75--92%. The proposed method can detect 0.5 micrograms/g feed, including corn, silage, ready mixed feeds, and feed pellets."}
{"id": "PMID:479081", "title": "Simultaneous extraction and fractionation and thin layer chromatographic determination of 14 mycotoxins in grains.", "content": "A simple, systematic analytical method for multiple mycotoxins was developed for detecting 14 mycotoxins; aflatoxins B1, B2, G1, and G2, sterigmatocystin, T-2 toxin, diacetoxyscirpenol, neosolaniol, fusarenon X, zearalenone, ochratoxin A, citrinin, luteoskyrin, and rugulosin. These mycotoxins were extracted with 20% H2SO4-4% KCl-acetonitrile (2 + 20 + 178), defatted with isooctane, and transferred to chloroform. The chloroform extract was cleaned up by silica gel column chromatography; the first 10 toxins were eluted with chloroform-methanol (97 + 3) and the remaining 4 toxins with benzene-acetone-acetic acid (75 + 20 + 5). Each fraction was analyzed by thin layer chromatography for the final determination. The method has been applied to polished rice, rough rice, corn, wheat, and peanuts as an analytical screening procedure. The detection limits in these commodities ranged from 10.00 to 800.0 microgram/kg, depending on the mycotoxin, but all limits were superior to those obtained for the individual mycotoxins by using other methods.", "contents": "Simultaneous extraction and fractionation and thin layer chromatographic determination of 14 mycotoxins in grains. A simple, systematic analytical method for multiple mycotoxins was developed for detecting 14 mycotoxins; aflatoxins B1, B2, G1, and G2, sterigmatocystin, T-2 toxin, diacetoxyscirpenol, neosolaniol, fusarenon X, zearalenone, ochratoxin A, citrinin, luteoskyrin, and rugulosin. These mycotoxins were extracted with 20% H2SO4-4% KCl-acetonitrile (2 + 20 + 178), defatted with isooctane, and transferred to chloroform. The chloroform extract was cleaned up by silica gel column chromatography; the first 10 toxins were eluted with chloroform-methanol (97 + 3) and the remaining 4 toxins with benzene-acetone-acetic acid (75 + 20 + 5). Each fraction was analyzed by thin layer chromatography for the final determination. The method has been applied to polished rice, rough rice, corn, wheat, and peanuts as an analytical screening procedure. The detection limits in these commodities ranged from 10.00 to 800.0 microgram/kg, depending on the mycotoxin, but all limits were superior to those obtained for the individual mycotoxins by using other methods."}
{"id": "PMID:479082", "title": "Thin layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin, and penitrem A.", "content": "A general method is described for determining 16 mycotoxins in mixed feeds and other food products used in the manufacture of these feedstuffs. The mycotoxins are extracted and cleaned up by extracting with solvents of different pH. Thin layer chromatography is used to separate the toxins; toxins are then quantitated by the limit detection method. The minimum detectable concentration of mycotoxins in various products is: aflatoxin B1 or G1, 4--5 micrograms/kg; ochratoxin A or ethyl ester A 140--145 micrograms/kg; citrinin 600--750 micrograms/kg; zearalenone, 410--500 micrograms/kg; sterigmatocystin, 140--145 micrograms/kg; diacetoxyscirpenol, 2400--2600 micrograms/kg; T-2 toxin, 800--950 micrograms/kg; patulin, 750--800 micrograms/kg; penitrem A 14,000--14,500 micrograms/kg; penicillic acid 3400--3650 micrograms/kg.", "contents": "Thin layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin, and penitrem A. A general method is described for determining 16 mycotoxins in mixed feeds and other food products used in the manufacture of these feedstuffs. The mycotoxins are extracted and cleaned up by extracting with solvents of different pH. Thin layer chromatography is used to separate the toxins; toxins are then quantitated by the limit detection method. The minimum detectable concentration of mycotoxins in various products is: aflatoxin B1 or G1, 4--5 micrograms/kg; ochratoxin A or ethyl ester A 140--145 micrograms/kg; citrinin 600--750 micrograms/kg; zearalenone, 410--500 micrograms/kg; sterigmatocystin, 140--145 micrograms/kg; diacetoxyscirpenol, 2400--2600 micrograms/kg; T-2 toxin, 800--950 micrograms/kg; patulin, 750--800 micrograms/kg; penitrem A 14,000--14,500 micrograms/kg; penicillic acid 3400--3650 micrograms/kg."}
{"id": "PMID:479083", "title": "High pressure liquid chromatographic determination of aflatoxins in corn.", "content": "A high pressure liquid chromatographic (HPLC) method is proposed for determining aflatoxins in corn. The sample is extracted with methanol-10% NaCl (4 + 1), pigments are precipitated with zinc acetate, and the extract is cleaned up on a small (2 g) silica gel column. Aflatoxins in the purified extract are resolved by normal phase HPLC on a microparticulate (10 micrometer) silica gel column with water-saturated chloroform-cyclohexane, acetonitrile solvent, and detected by fluorescence on a silica gel-packed flowcell. The method was compared with chloroform-water extraction of the official CB method on 15 samples of contaminated corn. In 5 of the 6 samples containing aflatoxins B1, B2, G1, and G2, methanol-10% NaCl extracted more aflatoxin than did cloroform-water, as measured both by HPLC and by thin layer chromatography. In samples containing only B1 and B2, the 2 extraction solvents were virtually equivalent. Agreement was good between HPLC and TLC for each extraction solvent. Average recovery of aflatoxins B1, B2, G1, and G2 added to yellow cornmeal at 3 levels was greater than 90%.", "contents": "High pressure liquid chromatographic determination of aflatoxins in corn. A high pressure liquid chromatographic (HPLC) method is proposed for determining aflatoxins in corn. The sample is extracted with methanol-10% NaCl (4 + 1), pigments are precipitated with zinc acetate, and the extract is cleaned up on a small (2 g) silica gel column. Aflatoxins in the purified extract are resolved by normal phase HPLC on a microparticulate (10 micrometer) silica gel column with water-saturated chloroform-cyclohexane, acetonitrile solvent, and detected by fluorescence on a silica gel-packed flowcell. The method was compared with chloroform-water extraction of the official CB method on 15 samples of contaminated corn. In 5 of the 6 samples containing aflatoxins B1, B2, G1, and G2, methanol-10% NaCl extracted more aflatoxin than did cloroform-water, as measured both by HPLC and by thin layer chromatography. In samples containing only B1 and B2, the 2 extraction solvents were virtually equivalent. Agreement was good between HPLC and TLC for each extraction solvent. Average recovery of aflatoxins B1, B2, G1, and G2 added to yellow cornmeal at 3 levels was greater than 90%."}
{"id": "PMID:479084", "title": "Mineral oil extraction of light filth from rubbed sage: collaborative study.", "content": "The official methods for extracting light filth from rubbed sage, 44.D08--44.D10, specifies a hot isopropanol pretreatment and flotation from cooled dilute isopropanol with mineral oil to extract light filth. The method gives good recoveries, but occasionally excessive interfering plant material is extracted along with the filth elements. A new method has been developed in which chloroform is used for pretreatment instead of isopropanol, and Tween 80-EDTA is added twice rather than once. This method has given consistently cleaner filter papers and better recoveries for rodent hairs, 85 vs. 79%, and for elytral squares, 95 vs. 84%. The new method has been adopted as official first action.", "contents": "Mineral oil extraction of light filth from rubbed sage: collaborative study. The official methods for extracting light filth from rubbed sage, 44.D08--44.D10, specifies a hot isopropanol pretreatment and flotation from cooled dilute isopropanol with mineral oil to extract light filth. The method gives good recoveries, but occasionally excessive interfering plant material is extracted along with the filth elements. A new method has been developed in which chloroform is used for pretreatment instead of isopropanol, and Tween 80-EDTA is added twice rather than once. This method has given consistently cleaner filter papers and better recoveries for rodent hairs, 85 vs. 79%, and for elytral squares, 95 vs. 84%. The new method has been adopted as official first action."}
{"id": "PMID:479085", "title": "Extraction of light filth from canned green leafy vegetables: collaborative study.", "content": "The official first action method, 44.108, for the extraction of light filth from whole or chopped canned greens specifies a water-heptane flotation that results in the flotation of an excessive amount of interfering plant material and in low recoveries. A new method has been developed using a 40% isopropanol-water-mineral oil flotation. The new method yielded a decreased amount of plant material and 84.9% recovery of the aphid spike compared with 42.5% recovery of the spike by the official method. The new method has been adopted as official first action to replace the existing method, except for broccoli.", "contents": "Extraction of light filth from canned green leafy vegetables: collaborative study. The official first action method, 44.108, for the extraction of light filth from whole or chopped canned greens specifies a water-heptane flotation that results in the flotation of an excessive amount of interfering plant material and in low recoveries. A new method has been developed using a 40% isopropanol-water-mineral oil flotation. The new method yielded a decreased amount of plant material and 84.9% recovery of the aphid spike compared with 42.5% recovery of the spike by the official method. The new method has been adopted as official first action to replace the existing method, except for broccoli."}
{"id": "PMID:479086", "title": "New brine saturation technique for the extraction of light filth from rubbed sage, paprika, and corn meal: intralaboratory study.", "content": "A new and widely applicable technique for the isolation of light filth from food has been developed. It includes a simple alcohol defatting followed by a brine saturation of the product by heating and cooling and a subsequent trapping of the filth elements with olive oil and tap water. The methods circumvent the use of hazardous and expensive solvents and lengthy defatting and/or pre-treatment procedures. In an intralaboratory study, recoveries of elytral squares and rodent hair fragments added to paprika, rubbed sage, and corn meal ranged from 95.8 to 100%.", "contents": "New brine saturation technique for the extraction of light filth from rubbed sage, paprika, and corn meal: intralaboratory study. A new and widely applicable technique for the isolation of light filth from food has been developed. It includes a simple alcohol defatting followed by a brine saturation of the product by heating and cooling and a subsequent trapping of the filth elements with olive oil and tap water. The methods circumvent the use of hazardous and expensive solvents and lengthy defatting and/or pre-treatment procedures. In an intralaboratory study, recoveries of elytral squares and rodent hair fragments added to paprika, rubbed sage, and corn meal ranged from 95.8 to 100%."}
{"id": "PMID:479087", "title": "Gas-liquid chromatographic determination of monosodium glutamate in soups and soup bases.", "content": "A method is given for determining monosodium glutamate (MSG) in soups and soup bases by gas-liquid chromatography (GLC) of the trimethylsilyl ether derivative of glutamic acid. This method compared favorably with existing methods including GLC of the trifluoroacetate (TFA)/butyl ester derivative, and analysis on an amino acid analyzer. In addition, some samples were analyzed by GLC/mass spectrometry. The MSG content of various products ranged from approximately 0.2% in some condensed soups to 13.1% on bouillon cubes. The method, which can detect as little as 0.05%, requires only a 10 min single step derivatization at room temperature and is preferred to the TFA/butyl ester technique.", "contents": "Gas-liquid chromatographic determination of monosodium glutamate in soups and soup bases. A method is given for determining monosodium glutamate (MSG) in soups and soup bases by gas-liquid chromatography (GLC) of the trimethylsilyl ether derivative of glutamic acid. This method compared favorably with existing methods including GLC of the trifluoroacetate (TFA)/butyl ester derivative, and analysis on an amino acid analyzer. In addition, some samples were analyzed by GLC/mass spectrometry. The MSG content of various products ranged from approximately 0.2% in some condensed soups to 13.1% on bouillon cubes. The method, which can detect as little as 0.05%, requires only a 10 min single step derivatization at room temperature and is preferred to the TFA/butyl ester technique."}
{"id": "PMID:479088", "title": "Colorimetric determination of boric acid in prawns, shrimp, and salted jelly fish by chelate extraction with 2-ethyl-1,3-hexanediol.", "content": "Borate was directly chelate-extracted from foods with 5% 2-ethyl-1,3-hexanediol (EHD) in n-hexane-n-butyl acetate mixture (8 + 2), from which borate was selectively transferred into 1% NaOH, since EHD-chelated boron did not react with curcumin to develop color. Finally, an aliquot of the alkaline solution was acidified with HCl and reacted with curcumin in a rotary evaporator. Color development was increased by heating for 8 min at 80 degrees C under reduced pressure of 16 mm Hg. Frozen shrimp and prawns (peeled and with shells) and salted jelly fish were analyzed by the proposed method. Results were compared with the contemporary official method of Japan based on curcumin reaction on an incinerated sample. Over 90% of the boric acid was recovered by the proposed method when samples were fortified with 20 ppm boric acid. Recoveries were superior to those of the official method especially for shrimp and prawns with shells and salted jelly fish. Detection limit of boric acid is 1 ppm. Moreover, the method requires only about 1 hr for analysis of one sample, making it suitable for routine analysis.", "contents": "Colorimetric determination of boric acid in prawns, shrimp, and salted jelly fish by chelate extraction with 2-ethyl-1,3-hexanediol. Borate was directly chelate-extracted from foods with 5% 2-ethyl-1,3-hexanediol (EHD) in n-hexane-n-butyl acetate mixture (8 + 2), from which borate was selectively transferred into 1% NaOH, since EHD-chelated boron did not react with curcumin to develop color. Finally, an aliquot of the alkaline solution was acidified with HCl and reacted with curcumin in a rotary evaporator. Color development was increased by heating for 8 min at 80 degrees C under reduced pressure of 16 mm Hg. Frozen shrimp and prawns (peeled and with shells) and salted jelly fish were analyzed by the proposed method. Results were compared with the contemporary official method of Japan based on curcumin reaction on an incinerated sample. Over 90% of the boric acid was recovered by the proposed method when samples were fortified with 20 ppm boric acid. Recoveries were superior to those of the official method especially for shrimp and prawns with shells and salted jelly fish. Detection limit of boric acid is 1 ppm. Moreover, the method requires only about 1 hr for analysis of one sample, making it suitable for routine analysis."}
{"id": "PMID:479089", "title": "Survey of some market basket commodities for polynuclear aromatic hydrocarbon content.", "content": "The Food and Drug Administration multi-component regulatory procedure for determining polynuclear aromatic hydrocarbons in foods was recently used to survey 24 foodstuffs for the presence of these compounds. The procedure has a reliable limit of quantitation of 2 ppb. The potent carcinogen benzo(a)pyrene was detected in only one of the 24 products analyzed, at a level of 3 ppb. Pyrene and/or fluoranthene were found in 19 of the 24 samples examined, at levels ranging from less than 1 ppb to about 75 ppb. A comparison of the recent survey data to that obtained approximately 10 years ago reveals that the types and levels of polynuclear aromatic hydrocarbons found are essentially unchanged.", "contents": "Survey of some market basket commodities for polynuclear aromatic hydrocarbon content. The Food and Drug Administration multi-component regulatory procedure for determining polynuclear aromatic hydrocarbons in foods was recently used to survey 24 foodstuffs for the presence of these compounds. The procedure has a reliable limit of quantitation of 2 ppb. The potent carcinogen benzo(a)pyrene was detected in only one of the 24 products analyzed, at a level of 3 ppb. Pyrene and/or fluoranthene were found in 19 of the 24 samples examined, at levels ranging from less than 1 ppb to about 75 ppb. A comparison of the recent survey data to that obtained approximately 10 years ago reveals that the types and levels of polynuclear aromatic hydrocarbons found are essentially unchanged."}
{"id": "PMID:479090", "title": "Determination of amprolium in feeds: collaborative study.", "content": "The official final action method, 42.028--42.032, for determining amprolium in feeds was modified by a change in the preparation of aluminum oxide for chromatography. A premix containing 0.5% amprolium was collaboratively studied by the modified and the official methods. Compared with the modified method, 87.7% of the drug was recovered from the premix by using the official method. The modification makes possible the assay of premixes as well as finished feeds. The official final action method has been modified to incorporate this change.", "contents": "Determination of amprolium in feeds: collaborative study. The official final action method, 42.028--42.032, for determining amprolium in feeds was modified by a change in the preparation of aluminum oxide for chromatography. A premix containing 0.5% amprolium was collaboratively studied by the modified and the official methods. Compared with the modified method, 87.7% of the drug was recovered from the premix by using the official method. The modification makes possible the assay of premixes as well as finished feeds. The official final action method has been modified to incorporate this change."}
{"id": "PMID:479091", "title": "High pressure liquid chromatographic determination of vitamins A and E in cereal products.", "content": "A rapid method for the simultaneous determination of vitamins A and E in fortified cereal products has been developed. Saponification of retinyl or tocopheryl esters is not required, permitting direct injection of the extracted lipids onto the high pressure liquid chromatographic column without sample cleanup. Elution times of 2.46 and 3.40 min were determined for retinyl palmitate and tocopheryl acetate, respectively, using a muPorasil column and an isocratic mobile phase of hexane-chloroform (85 + 15) with a flow rate of 1.5 mL/min. The average recovery of retinyl palmitate was 99.2% (std dev. 4.28), and the average recovery of tocopheryl acetate was 94.9% (std dev. 4.10) in 2 cereals containing corn, oat, rice, and wheat. No significant amounts of naturally occurring tocopherols were found in the cereals.", "contents": "High pressure liquid chromatographic determination of vitamins A and E in cereal products. A rapid method for the simultaneous determination of vitamins A and E in fortified cereal products has been developed. Saponification of retinyl or tocopheryl esters is not required, permitting direct injection of the extracted lipids onto the high pressure liquid chromatographic column without sample cleanup. Elution times of 2.46 and 3.40 min were determined for retinyl palmitate and tocopheryl acetate, respectively, using a muPorasil column and an isocratic mobile phase of hexane-chloroform (85 + 15) with a flow rate of 1.5 mL/min. The average recovery of retinyl palmitate was 99.2% (std dev. 4.28), and the average recovery of tocopheryl acetate was 94.9% (std dev. 4.10) in 2 cereals containing corn, oat, rice, and wheat. No significant amounts of naturally occurring tocopherols were found in the cereals."}
{"id": "PMID:479092", "title": "Automated fluorometric determination of thiamine and riboflavin in infant formulas.", "content": "Commercial infant formulas were analyzed simultaneously for thiamine and riboflavin by an automated fluorometric method and by the AOAC manual fluorometric methods. For 10 products, the mean thiamine and riboflavin results determined using the automated method ranged from 104 to 113% and 90 to 112%, respectively, of those by the AOAC manual methods. The coefficients of variation for thiamine and riboflavin ranged from 1.05 to 3.90% and 0.60 to 2.48%, respectively, for the automated methods, and 1.48 to 3.86% and 0.69 to 10.9%, respectively, for the manual methods. Using the automated method, mean recoveries of thiamine and riboflavin added to samples were 103 and 104%, respectively. The automated method used a common sample preparation to determine both thiamine and riboflavin, and gave results equivalent to, or better than, those obtained by the manual methods.", "contents": "Automated fluorometric determination of thiamine and riboflavin in infant formulas. Commercial infant formulas were analyzed simultaneously for thiamine and riboflavin by an automated fluorometric method and by the AOAC manual fluorometric methods. For 10 products, the mean thiamine and riboflavin results determined using the automated method ranged from 104 to 113% and 90 to 112%, respectively, of those by the AOAC manual methods. The coefficients of variation for thiamine and riboflavin ranged from 1.05 to 3.90% and 0.60 to 2.48%, respectively, for the automated methods, and 1.48 to 3.86% and 0.69 to 10.9%, respectively, for the manual methods. Using the automated method, mean recoveries of thiamine and riboflavin added to samples were 103 and 104%, respectively. The automated method used a common sample preparation to determine both thiamine and riboflavin, and gave results equivalent to, or better than, those obtained by the manual methods."}
{"id": "PMID:479093", "title": "Automated fluorometric determination of vitamin C in foods.", "content": "Two automated fluorometric methods were compared with the official AOAC methods for determining vitamin C in fortified ready-to-eat cereals, fruits, vegetables, baby foods, flour products, pet foods, meats, frozen dinners, juices, and nutritional health bars. Each sample was analyzed in duplicate on 2 days and included a recovery study on all products. Egberg's semiautomated method (Method 1) and Roy's automated method (Method 2) were compared with the manual AOAC tritrimetric and fluorometric methods. The correlation factor for Methods 1 and 2 were 0.999 and 0.979, respectively, when compared with the AOAC methods. The recovery study showed average recoveries of 97.8% for Method 1, 99.3% for Method 2, and 100.6% for the AOAC methods. The results suggest that Method 1 is the method of choice for the majority of the products analyzed.", "contents": "Automated fluorometric determination of vitamin C in foods. Two automated fluorometric methods were compared with the official AOAC methods for determining vitamin C in fortified ready-to-eat cereals, fruits, vegetables, baby foods, flour products, pet foods, meats, frozen dinners, juices, and nutritional health bars. Each sample was analyzed in duplicate on 2 days and included a recovery study on all products. Egberg's semiautomated method (Method 1) and Roy's automated method (Method 2) were compared with the manual AOAC tritrimetric and fluorometric methods. The correlation factor for Methods 1 and 2 were 0.999 and 0.979, respectively, when compared with the AOAC methods. The recovery study showed average recoveries of 97.8% for Method 1, 99.3% for Method 2, and 100.6% for the AOAC methods. The results suggest that Method 1 is the method of choice for the majority of the products analyzed."}
{"id": "PMID:479094", "title": "Pyridine extraction method for determination of bacitracin in feeds: collaborative evaluation.", "content": "Seventeen laboratories evaluated the pyridine extraction method and neomycin-sensitized agar for the determination of zinc and MD bacitracin in swine and broiler rations at 10 and 100 g/ton. The method was also applied to the analysis of 2 premixes labeled 50 g/lb (MD bacitracin) and 40 g/lb (zinc bacitracin). Bacitracin activity was determined on each of 2 days with 2 dilutions on each day. No significant difference was found between dilutions within a day or between days for each sample. The type of bacitracin or type of feed did not significantly affect results. The difference in results between MD and zinc bacitracin in premixes approached significance. The large coefficients of variation for premixes (ca 13%) and complete feeds (ca 15--30%) indicate operational problems. The main difficulty was evaporation of pyridine. Some laboratories were not able to evaporate it completely, whereas others lost bacitracin activity, probably due to high temperature of drying. The pyridine extraction method as in 42.200 and 42.204 should be discontinued.", "contents": "Pyridine extraction method for determination of bacitracin in feeds: collaborative evaluation. Seventeen laboratories evaluated the pyridine extraction method and neomycin-sensitized agar for the determination of zinc and MD bacitracin in swine and broiler rations at 10 and 100 g/ton. The method was also applied to the analysis of 2 premixes labeled 50 g/lb (MD bacitracin) and 40 g/lb (zinc bacitracin). Bacitracin activity was determined on each of 2 days with 2 dilutions on each day. No significant difference was found between dilutions within a day or between days for each sample. The type of bacitracin or type of feed did not significantly affect results. The difference in results between MD and zinc bacitracin in premixes approached significance. The large coefficients of variation for premixes (ca 13%) and complete feeds (ca 15--30%) indicate operational problems. The main difficulty was evaporation of pyridine. Some laboratories were not able to evaporate it completely, whereas others lost bacitracin activity, probably due to high temperature of drying. The pyridine extraction method as in 42.200 and 42.204 should be discontinued."}
{"id": "PMID:479095", "title": "Determination of chlorinated pesticide residues in foods. I. Rapid screening method for chlorinated pesticides in milk.", "content": "A rapid analytical method for determining chlorinated pesticide residues in milk was developed. Thirteen pesticides were almost completely extracted. Ten mL samples of fortified milk were extracted 3 times with 20 mL portions of n-hexane as follows: (A) in the absence of water-soluble solvent; in the presence of (B) 1 mL acetonitrile; (C) 3 mL acetonitrile; (D) 5 mL acetonitrile; (E) 5 mL ethanol; (F) 5 mL acetonitrile and 1 mL ethanol. System F produced the highest pesticide recoveries but the lowest fat extraction, thus eliminating the necessity for liquid-liquid partitioning and minimizing Florisil column cleanup. Pesticide recoveries throughout the procedure were 94--103%. It was noticed, however, that the fat in high fat-containing raw milk is more readily extracted than that in commercial milk.", "contents": "Determination of chlorinated pesticide residues in foods. I. Rapid screening method for chlorinated pesticides in milk. A rapid analytical method for determining chlorinated pesticide residues in milk was developed. Thirteen pesticides were almost completely extracted. Ten mL samples of fortified milk were extracted 3 times with 20 mL portions of n-hexane as follows: (A) in the absence of water-soluble solvent; in the presence of (B) 1 mL acetonitrile; (C) 3 mL acetonitrile; (D) 5 mL acetonitrile; (E) 5 mL ethanol; (F) 5 mL acetonitrile and 1 mL ethanol. System F produced the highest pesticide recoveries but the lowest fat extraction, thus eliminating the necessity for liquid-liquid partitioning and minimizing Florisil column cleanup. Pesticide recoveries throughout the procedure were 94--103%. It was noticed, however, that the fat in high fat-containing raw milk is more readily extracted than that in commercial milk."}
{"id": "PMID:479096", "title": "Determination of chlorinated pesticide residues in foods. II. Potassium permanganate oxidation for cleanup of some vegetable extracts.", "content": "A potassium permanganate-dilute sulfuric acid KMnO4/dilute H2SO4 oxidation procedure was developed to supplement Florisil cleanup of some vegetable extracts. Following sample preparation and Florisil cleanup, a reaction mixture of the n-hexane eluate from the Florisil cleanup, 4% KMnO4, and 40% H2SO4 (1 + 1 + 1) was shaken in a test tube 2 min at room temperature and then centrifuged. The n-hexane phase was washed with 2 mL 0.1N NaOH and analyzed by GLC. Twelve chlorinated pesticides were completely recovered in the n-hexane phase. Aldrin was not recovered because its extreme instability caused it to decompose even in neutral solutions. Chlorinated pesticide residues in onion, garlic, carrot, and radish root were easily analyzed by the application of this oxidation procedure.", "contents": "Determination of chlorinated pesticide residues in foods. II. Potassium permanganate oxidation for cleanup of some vegetable extracts. A potassium permanganate-dilute sulfuric acid KMnO4/dilute H2SO4 oxidation procedure was developed to supplement Florisil cleanup of some vegetable extracts. Following sample preparation and Florisil cleanup, a reaction mixture of the n-hexane eluate from the Florisil cleanup, 4% KMnO4, and 40% H2SO4 (1 + 1 + 1) was shaken in a test tube 2 min at room temperature and then centrifuged. The n-hexane phase was washed with 2 mL 0.1N NaOH and analyzed by GLC. Twelve chlorinated pesticides were completely recovered in the n-hexane phase. Aldrin was not recovered because its extreme instability caused it to decompose even in neutral solutions. Chlorinated pesticide residues in onion, garlic, carrot, and radish root were easily analyzed by the application of this oxidation procedure."}
{"id": "PMID:479097", "title": "Determination of chlorinated pesticide residues in foods. II. Simultaneous analysis of chlorinated pesticide and phthalate ester residues by using AgNO3-coated Florisil column chromatography for cleanup of various samples.", "content": "A simplified method suitable for simultaneous analysis of chlorinated pesticide and phthalate ester residues in various foods was developed. Chemical residues were quantitatively extracted from fatty and vegetable samples with acetonitrile as follows: Chemical standard in 0.5 mL ethanol solution was added to 10 g homogenized sample. After 3 hr, pork and beef were extracted 3 times with 20 mL portions of acetonitrile. The acetonitrile layers were diluted with water and extracted with n-hexane. Rice samples were combined with 10 mL water, 5 mL acetonitrile and 1 mL ethanol and extracted 3 times with 20 mL portions of n-hexane. The n-hexane concentrate from each sample was submitted to AgNO3-coated Florisil column chromatography. The AgNO3 coating adequately adsorbed interfering coextractives. Extracts of fish and vegetable samples were separated into 2 fractions by the above column chromatography. Supplemental cleanup procedures were also developed to accurately determine phthalate esters eluted in the second fraction. Satisfactory gas chromatograms were obtained for most samples.", "contents": "Determination of chlorinated pesticide residues in foods. II. Simultaneous analysis of chlorinated pesticide and phthalate ester residues by using AgNO3-coated Florisil column chromatography for cleanup of various samples. A simplified method suitable for simultaneous analysis of chlorinated pesticide and phthalate ester residues in various foods was developed. Chemical residues were quantitatively extracted from fatty and vegetable samples with acetonitrile as follows: Chemical standard in 0.5 mL ethanol solution was added to 10 g homogenized sample. After 3 hr, pork and beef were extracted 3 times with 20 mL portions of acetonitrile. The acetonitrile layers were diluted with water and extracted with n-hexane. Rice samples were combined with 10 mL water, 5 mL acetonitrile and 1 mL ethanol and extracted 3 times with 20 mL portions of n-hexane. The n-hexane concentrate from each sample was submitted to AgNO3-coated Florisil column chromatography. The AgNO3 coating adequately adsorbed interfering coextractives. Extracts of fish and vegetable samples were separated into 2 fractions by the above column chromatography. Supplemental cleanup procedures were also developed to accurately determine phthalate esters eluted in the second fraction. Satisfactory gas chromatograms were obtained for most samples."}
{"id": "PMID:479098", "title": "Do autistic children come from upper-middle-class parents?", "content": "Most studies have reported autistic children as coming from upper social economic status (SES) families. A few studies have not found any such social class bias. In order to resolve these contradictory findings, an empirical study was conducted on a statewide sample of families with autistic children. It was hypothesized that autistic children from high SES families would be associated with seven social class selection factors: (1) early age of onset, (2) early age of treatment admission, (3) normal cognitive potential, (4) complex rituals with maintenance of sameness, (5) long distance traveled for treatment, (6) limited availability of services, and (7) very detailed child history. Factors 1, 5, 6, and 7 distinguished high SES from low SES families in the predicted direction. Implications for research and treatment are discussed.", "contents": "Do autistic children come from upper-middle-class parents? Most studies have reported autistic children as coming from upper social economic status (SES) families. A few studies have not found any such social class bias. In order to resolve these contradictory findings, an empirical study was conducted on a statewide sample of families with autistic children. It was hypothesized that autistic children from high SES families would be associated with seven social class selection factors: (1) early age of onset, (2) early age of treatment admission, (3) normal cognitive potential, (4) complex rituals with maintenance of sameness, (5) long distance traveled for treatment, (6) limited availability of services, and (7) very detailed child history. Factors 1, 5, 6, and 7 distinguished high SES from low SES families in the predicted direction. Implications for research and treatment are discussed."}
{"id": "PMID:479099", "title": "Autism and unfavorable left-right asymmetries of the brain.", "content": "Utilizing computerized brain tomography, left-right morphologic asymmetries of the parietooccipital region were judged in 16 autistic patients, 44 mentally retarded patients, and 100 miscellaneous neurological patients. In 57% of the autistic patients the right parietooccipital region was wider than the left, while this pattern of cerebral asymmetry was found in only 23% of the mentally retarded patients and 25% of the neurological patients. It is suggested that unfavorable morphologic asymmetries of the brain near the posterior language zone may contribute to the difficulties autistic children experience in acquiring language.", "contents": "Autism and unfavorable left-right asymmetries of the brain. Utilizing computerized brain tomography, left-right morphologic asymmetries of the parietooccipital region were judged in 16 autistic patients, 44 mentally retarded patients, and 100 miscellaneous neurological patients. In 57% of the autistic patients the right parietooccipital region was wider than the left, while this pattern of cerebral asymmetry was found in only 23% of the mentally retarded patients and 25% of the neurological patients. It is suggested that unfavorable morphologic asymmetries of the brain near the posterior language zone may contribute to the difficulties autistic children experience in acquiring language."}
{"id": "PMID:479100", "title": "Transformational deficits in cognition of schizophrenic children.", "content": "This study examined schizophrenic children for cognitive deficits in logical thinking, symbolic imagery, and conservation. Evaluation revealed persistent failure of the aberrant subjects to achieve at the level of age expectancy. Comparison with nonpsychotic, institutionalized children demonstrated no effect due to institutionalization. By mid-teens not one of the subjects had accomplished formal operations and very few were able to master concrete operations. Implications for development and treatment were discussed.", "contents": "Transformational deficits in cognition of schizophrenic children. This study examined schizophrenic children for cognitive deficits in logical thinking, symbolic imagery, and conservation. Evaluation revealed persistent failure of the aberrant subjects to achieve at the level of age expectancy. Comparison with nonpsychotic, institutionalized children demonstrated no effect due to institutionalization. By mid-teens not one of the subjects had accomplished formal operations and very few were able to master concrete operations. Implications for development and treatment were discussed."}
{"id": "PMID:479101", "title": "Adult recollections of a formerly autistic child.", "content": "The life history, current status, and memories of a 31-year-old man who, as a child, had been diagnosed as autistic by Kanner and others is described. Prognostic criteria as related to this individual's outcome are discussed. Some speculations about the inner life of autistic children are advanced on the basis of his recollections. Finally, some hypotheses for further research are suggested on the basis of information gained from this case review.", "contents": "Adult recollections of a formerly autistic child. The life history, current status, and memories of a 31-year-old man who, as a child, had been diagnosed as autistic by Kanner and others is described. Prognostic criteria as related to this individual's outcome are discussed. Some speculations about the inner life of autistic children are advanced on the basis of his recollections. Finally, some hypotheses for further research are suggested on the basis of information gained from this case review."}
{"id": "PMID:479103", "title": "Surface arrays on the wall of Sporosarcina ureae.", "content": "Thin sections of the cell wall of Sporosarcina ureae revealed two structurally distinct layers: a continuous amorphous zone, approximately 15 nm thick, which was adjacent to the plasma membrane, and an overlying periodic zone, approximately 16 nm thick. Sequential Triton X-100 and lysozyme treatment of isolated walls produced small fragments of the outer regular structure which allowed high-resolution, negatively stained images suitable for optical diffractometric analysis. These data suggested a tetragonal array of complex polygonal units of C-C spacing = 12 nm, with each unit joined to another by two delicate linkers. The array was entirely proteinaceous, consisting of a 150,000-dalton polypeptide which had a high affinity for Mg2+. It proved to be sensitive to chelating agents, 5 mM concentrations of Ca2+, Sr2+, or Ba2+, proteases, heat greater than or equal to 45 degrees C, sodium dodecyl sulfate, and pH greater than or equal to 5.8, but magnesium offered protection against the chelating agents and the deleterious salts.", "contents": "Surface arrays on the wall of Sporosarcina ureae. Thin sections of the cell wall of Sporosarcina ureae revealed two structurally distinct layers: a continuous amorphous zone, approximately 15 nm thick, which was adjacent to the plasma membrane, and an overlying periodic zone, approximately 16 nm thick. Sequential Triton X-100 and lysozyme treatment of isolated walls produced small fragments of the outer regular structure which allowed high-resolution, negatively stained images suitable for optical diffractometric analysis. These data suggested a tetragonal array of complex polygonal units of C-C spacing = 12 nm, with each unit joined to another by two delicate linkers. The array was entirely proteinaceous, consisting of a 150,000-dalton polypeptide which had a high affinity for Mg2+. It proved to be sensitive to chelating agents, 5 mM concentrations of Ca2+, Sr2+, or Ba2+, proteases, heat greater than or equal to 45 degrees C, sodium dodecyl sulfate, and pH greater than or equal to 5.8, but magnesium offered protection against the chelating agents and the deleterious salts."}
{"id": "PMID:479104", "title": "Optimal conditions for transformation of Azotobacter vinelandii.", "content": "Optimal transformation of Azotobacter vinelandii OP required a 20-min incubation of the competent cells with deoxyribonucleic acid at 30 degrees C in buffer (pH 6.0 to 8.0) containing 8 mM magnesium sulfate. Nitrogen-fixing transformants of nitrogen fixation-deficient recipients could be plated immediately on selective medium, but transformants acquiring rifampin and streptomycin resistance required preincubation in nonselective medium. The three phenotypes achieved an approximately equal and stable frequency after 17 h (six generations) of growth in nonselective medium.", "contents": "Optimal conditions for transformation of Azotobacter vinelandii. Optimal transformation of Azotobacter vinelandii OP required a 20-min incubation of the competent cells with deoxyribonucleic acid at 30 degrees C in buffer (pH 6.0 to 8.0) containing 8 mM magnesium sulfate. Nitrogen-fixing transformants of nitrogen fixation-deficient recipients could be plated immediately on selective medium, but transformants acquiring rifampin and streptomycin resistance required preincubation in nonselective medium. The three phenotypes achieved an approximately equal and stable frequency after 17 h (six generations) of growth in nonselective medium."}
{"id": "PMID:479105", "title": "Transfer of kanamycin resistance mediated by plasmid R68.45 in Paracoccus denitrificans.", "content": "Plasmid R68.45 mediates the transfer of kanamycin resistance from Pseudomonas aeruginosa to Paracoccus denitrificans. Kanamycin resistance could be transferred from one strain of P. denitrificans to another, thus opening up the possibility of using R68.45 as a sex factor in P. denitrificans.", "contents": "Transfer of kanamycin resistance mediated by plasmid R68.45 in Paracoccus denitrificans. Plasmid R68.45 mediates the transfer of kanamycin resistance from Pseudomonas aeruginosa to Paracoccus denitrificans. Kanamycin resistance could be transferred from one strain of P. denitrificans to another, thus opening up the possibility of using R68.45 as a sex factor in P. denitrificans."}
{"id": "PMID:479106", "title": "Effect of glucose on polyol metabolism by Rhizobium trifolii.", "content": "The effect of glucose on polyol metabolism by Rhizobium trifolii was studied. Phenomena similar to catabolite repression and catabolite inhibition were observed. The catabolism of glucose to at least glucose 6-phosphate was required for the effects to be exerted.", "contents": "Effect of glucose on polyol metabolism by Rhizobium trifolii. The effect of glucose on polyol metabolism by Rhizobium trifolii was studied. Phenomena similar to catabolite repression and catabolite inhibition were observed. The catabolism of glucose to at least glucose 6-phosphate was required for the effects to be exerted."}
{"id": "PMID:479107", "title": "Transport of C5 dicarboxylate compounds by Pseudomonas putida.", "content": "Induced glutarate and 2-oxoglutarate uptake and transport by Pseudomonas putida were investigated in whole cells and membrane vesicles, respectively. Uptake of 2-oxoglutarate, but not glutarate, was against a concentration gradient to 1.7-fold greater than the initial extracellular concentration. Membrane vesicles transported 2-oxoglutarate and glutarate against gradients to intramembrane concentrations fivefold greater than the initial extravesicle concentrations. The rates of transport of both compounds were greatest in the presence of the artificial electron donor system phenazine methosulfate-ascorbate. Malate and D-lactate were the only naturally occurring compounds that served as electron donors. Uptake and transport were inhibited by KCN, NaN3, and 2,2-dinitrophenol. Kinetic parameters of transport were: glutarate, apparent Km--1.22 mM, Vmax--400 nmol/min per mg of membrane protein; 2-oxoglutarate, apparent Km--131 microM, Vmax--255 nmol/min per mg of membrane protein. Studies of competitive inhibition indicated a common system for transport of five C5 dicarboxylate compounds. The apparent Km and Ki values with 2-oxoglutarate as a substrate placed the substrate affinity for transport in the order 2-oxoglutarate greater than glutarate greater than D-2-hydroxyglutarate and L-2-hydroxyglutarate greater than glutaconate.", "contents": "Transport of C5 dicarboxylate compounds by Pseudomonas putida. Induced glutarate and 2-oxoglutarate uptake and transport by Pseudomonas putida were investigated in whole cells and membrane vesicles, respectively. Uptake of 2-oxoglutarate, but not glutarate, was against a concentration gradient to 1.7-fold greater than the initial extracellular concentration. Membrane vesicles transported 2-oxoglutarate and glutarate against gradients to intramembrane concentrations fivefold greater than the initial extravesicle concentrations. The rates of transport of both compounds were greatest in the presence of the artificial electron donor system phenazine methosulfate-ascorbate. Malate and D-lactate were the only naturally occurring compounds that served as electron donors. Uptake and transport were inhibited by KCN, NaN3, and 2,2-dinitrophenol. Kinetic parameters of transport were: glutarate, apparent Km--1.22 mM, Vmax--400 nmol/min per mg of membrane protein; 2-oxoglutarate, apparent Km--131 microM, Vmax--255 nmol/min per mg of membrane protein. Studies of competitive inhibition indicated a common system for transport of five C5 dicarboxylate compounds. The apparent Km and Ki values with 2-oxoglutarate as a substrate placed the substrate affinity for transport in the order 2-oxoglutarate greater than glutarate greater than D-2-hydroxyglutarate and L-2-hydroxyglutarate greater than glutaconate."}
{"id": "PMID:479108", "title": "Plasmenylethanolamine: growth factor for cholesterol-reducing Eubacterium.", "content": "A plasmalogen, plasmenylethanolamine, is required for in vitro growth of strains of Eubacterium which convert cholesterol to coprostanol. Plasmenylethanolamine was isolated from calf brain by selective saponification of lipid fractions separated by thin-layer or column chromatography. Cholesterol-containing thioglycolate broth plus purified plasmenylethanolamine or its 2-lyso derivative supported growth of Eubacterium ATCC 21408 and a cholesterol-reducing Eubacterium isolated from baboon feces. Plasmenylethanolamine obtained from commercial sources also supported growth of these organisms, but none of a number of other pure lipids would support growth. Metabolism of the alkenyl ether group of plasmenylethanolamine occurred during growth.", "contents": "Plasmenylethanolamine: growth factor for cholesterol-reducing Eubacterium. A plasmalogen, plasmenylethanolamine, is required for in vitro growth of strains of Eubacterium which convert cholesterol to coprostanol. Plasmenylethanolamine was isolated from calf brain by selective saponification of lipid fractions separated by thin-layer or column chromatography. Cholesterol-containing thioglycolate broth plus purified plasmenylethanolamine or its 2-lyso derivative supported growth of Eubacterium ATCC 21408 and a cholesterol-reducing Eubacterium isolated from baboon feces. Plasmenylethanolamine obtained from commercial sources also supported growth of these organisms, but none of a number of other pure lipids would support growth. Metabolism of the alkenyl ether group of plasmenylethanolamine occurred during growth."}
{"id": "PMID:479109", "title": "Consequences of Ca2+ deficiency on macromolecular synthesis and adenylate energy charge in Yersinia pestis.", "content": "A 37 but not 26 degrees C virulent Yersinia pestis is known to require at least 2.5 mM Ca2+ for growth; this requirement is potentiated by Mg2+. After shift of log-phase cells (doubling time of 2 h) from 26 to 37 degrees C in Ca2+-deficient medium, shutoff of net ribonucleic acid synthesis preceded that of protein and cell mass. With 2.5 mM Mg2+, about two doublings in cell mass and number occurred before restriction with synthesis of sufficient deoxyribonucleic acid to account for initiation and termination of two postshift rounds of chromosome replication. Temperature shift with 20 mMMg2+ resulted in a single doubling of cell mass and number with one round of chromosome replication. Subsequent to shutoff of ribonucleic acid accumulation, ribonucleoside but not deoxyribonucleoside triphosphate pools became reduced to about 50% of normal values and the adenylate energy change fell from about 0.8, typical of growing cells, to about 0.6. Excretion of significant concentrations of adenine nucleotides under both permissive and restrictive conditions was observed. Only trace levels (less than 0.01 microM ol/g [dry weight]) of guaninosine 5'-diphosphate 3'-diphosphate accumulated under restrictive or permissive conditions; guanosine 5'-triphosphate 3'-diphosphate was not detected. Return of fully restricted cells from 37 to 26 degrees C with Ca2+ resulted in prompt growth, whereas addition of Ca2+ at 37 degrees C was ineffective. This finding indicates that the observed temperature-sensitive lesion in ribonucleic acid synthesis that results in restriction can be prevented but not reversed by cultivation with Ca2+.", "contents": "Consequences of Ca2+ deficiency on macromolecular synthesis and adenylate energy charge in Yersinia pestis. A 37 but not 26 degrees C virulent Yersinia pestis is known to require at least 2.5 mM Ca2+ for growth; this requirement is potentiated by Mg2+. After shift of log-phase cells (doubling time of 2 h) from 26 to 37 degrees C in Ca2+-deficient medium, shutoff of net ribonucleic acid synthesis preceded that of protein and cell mass. With 2.5 mM Mg2+, about two doublings in cell mass and number occurred before restriction with synthesis of sufficient deoxyribonucleic acid to account for initiation and termination of two postshift rounds of chromosome replication. Temperature shift with 20 mMMg2+ resulted in a single doubling of cell mass and number with one round of chromosome replication. Subsequent to shutoff of ribonucleic acid accumulation, ribonucleoside but not deoxyribonucleoside triphosphate pools became reduced to about 50% of normal values and the adenylate energy change fell from about 0.8, typical of growing cells, to about 0.6. Excretion of significant concentrations of adenine nucleotides under both permissive and restrictive conditions was observed. Only trace levels (less than 0.01 microM ol/g [dry weight]) of guaninosine 5'-diphosphate 3'-diphosphate accumulated under restrictive or permissive conditions; guanosine 5'-triphosphate 3'-diphosphate was not detected. Return of fully restricted cells from 37 to 26 degrees C with Ca2+ resulted in prompt growth, whereas addition of Ca2+ at 37 degrees C was ineffective. This finding indicates that the observed temperature-sensitive lesion in ribonucleic acid synthesis that results in restriction can be prevented but not reversed by cultivation with Ca2+."}
{"id": "PMID:479110", "title": "Genetic mapping of toxin regulatory mutations in Vibrio cholerae.", "content": "We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used to construct improved genetic donors in 569B parental and hypertoxinogenic mutant strains. Subsequent mapping by conjugation indicated that the htx locus was closely linked to the rif, str, and ilv loci of V. cholerae. Analysis of recombinants from these crosses suggested the following gene order: thy str htx rif ilv arg. The close genetic linkage of htx to rif (as high as 98%) resulted in a high comutation frequency of these two loci by nitrosoguanidine mutagenesis. Transfer of the htx mutant locus from a hypertoxinogenic donor to several unrelated Tox+ strains of V. cholerae caused a detectable elevation of toxin production in the recipients. These results suggest that toxin production in diverse strains of V. cholerae is controlled by a common regulatory mechanism in which the htx gene product plays a significant role.", "contents": "Genetic mapping of toxin regulatory mutations in Vibrio cholerae. We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used to construct improved genetic donors in 569B parental and hypertoxinogenic mutant strains. Subsequent mapping by conjugation indicated that the htx locus was closely linked to the rif, str, and ilv loci of V. cholerae. Analysis of recombinants from these crosses suggested the following gene order: thy str htx rif ilv arg. The close genetic linkage of htx to rif (as high as 98%) resulted in a high comutation frequency of these two loci by nitrosoguanidine mutagenesis. Transfer of the htx mutant locus from a hypertoxinogenic donor to several unrelated Tox+ strains of V. cholerae caused a detectable elevation of toxin production in the recipients. These results suggest that toxin production in diverse strains of V. cholerae is controlled by a common regulatory mechanism in which the htx gene product plays a significant role."}
{"id": "PMID:479111", "title": "Insertion element analysis and mapping of the Pseudomonas plasmid alk regulon.", "content": "We characterized and mapped new mutations of the alk (alkane utilization) genes found on Pseudomonas plasmids of the Inc P-2 group. These mutations were isolated after (i) nitrosoguanidine mutagenesis, (ii) transposition of the Tn7 trimethoprim and streptomycin resistance determinant, and (iii) reversion of polarity effects of alk::Tn7 insertion mutations. Our results indicate the existence of two alk loci not previously described--alkD, whose product is required for synthesis of membrane alkane-oxidizing activities, and alkE, whose product is required for synthesis of inducible membrane alcohol dehydrogenase activity. Polarity of alk::Tn7 insertion mutations indicates the existence of an alkBAE operon. Mapping of alk loci by transduction in P. aeruginosa shows that there are at least three alk clusters in the CAM-OCT plasmid--alkRD, containing regulatory genes; alkBAE, containing genes for specific biochemical activities; and alkC, containing one or more genes needed for normal synthesis of membrane alcohol dehydrogenase. The alkRD and alkBAE clusters are linked but separated by about 42 kilobases. The alkC cluster is not linked to either of the other two alk regions. Altogether, these results indicate a complex genetic control of the alkane utilization phenotype in P. putida and P. aeruginosa involving at least six separate genes.", "contents": "Insertion element analysis and mapping of the Pseudomonas plasmid alk regulon. We characterized and mapped new mutations of the alk (alkane utilization) genes found on Pseudomonas plasmids of the Inc P-2 group. These mutations were isolated after (i) nitrosoguanidine mutagenesis, (ii) transposition of the Tn7 trimethoprim and streptomycin resistance determinant, and (iii) reversion of polarity effects of alk::Tn7 insertion mutations. Our results indicate the existence of two alk loci not previously described--alkD, whose product is required for synthesis of membrane alkane-oxidizing activities, and alkE, whose product is required for synthesis of inducible membrane alcohol dehydrogenase activity. Polarity of alk::Tn7 insertion mutations indicates the existence of an alkBAE operon. Mapping of alk loci by transduction in P. aeruginosa shows that there are at least three alk clusters in the CAM-OCT plasmid--alkRD, containing regulatory genes; alkBAE, containing genes for specific biochemical activities; and alkC, containing one or more genes needed for normal synthesis of membrane alcohol dehydrogenase. The alkRD and alkBAE clusters are linked but separated by about 42 kilobases. The alkC cluster is not linked to either of the other two alk regions. Altogether, these results indicate a complex genetic control of the alkane utilization phenotype in P. putida and P. aeruginosa involving at least six separate genes."}
{"id": "PMID:479112", "title": "High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol.", "content": "Conditions were established for the regeneration of protoplasts of Streptomyces parvulus and Streptomyces antibioticus to the mycelial form. Regeneration was accomplished with a hypertonic medium that contained sucrose, CaCl2, MgCl2, and low levels of phosphate. High-frequency fusion of protoplasts derived from auxotrophic strains of S. parvulus or S. antibioticus was induced by polyethylene glycol 4,000 (42%, wt/vol). The frequency of genetic transfer by the fusogenic procedure varied with the auxotrophic strains examined. Fusion with auxotrophic strains of S. parvulus resulted in the formation of true prototrophic recombinants. Similar studies with S. antibioticus revealed that both stable prototrophic recombinants and heterokaryons were formed.", "contents": "High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol. Conditions were established for the regeneration of protoplasts of Streptomyces parvulus and Streptomyces antibioticus to the mycelial form. Regeneration was accomplished with a hypertonic medium that contained sucrose, CaCl2, MgCl2, and low levels of phosphate. High-frequency fusion of protoplasts derived from auxotrophic strains of S. parvulus or S. antibioticus was induced by polyethylene glycol 4,000 (42%, wt/vol). The frequency of genetic transfer by the fusogenic procedure varied with the auxotrophic strains examined. Fusion with auxotrophic strains of S. parvulus resulted in the formation of true prototrophic recombinants. Similar studies with S. antibioticus revealed that both stable prototrophic recombinants and heterokaryons were formed."}
{"id": "PMID:479113", "title": "Minimal brain dysfunction in childhood: outcome in late adolescence and early adult years.", "content": "Seventy-three patients, diagnosed in childhood as having minimal brain dysfunction syndrome and further classified as either developmental lag (38%) or organic brain syndrome (62%), were followed into late adolescence and early adult life. At follow-up 7% were free of psychiatric disorder, 80% had various types of personality disorder, and 14% were borderline psychotic. Global outcome was rated as satisfactory in 20%, unsatisfactory in 80%. Associated with an unsatisfactory outcome were these initial findings: low normal or borderline intelligence, multiplicity of behavioral and neuropsychological findings, learning disabilities, special class placement, and initial classification of organic brain syndrome. Initial differentiation between developmental lag and organic brain syndrome foreshadowed different psychopathological features having significant polarities and little overlap, as well as different global outcome.", "contents": "Minimal brain dysfunction in childhood: outcome in late adolescence and early adult years. Seventy-three patients, diagnosed in childhood as having minimal brain dysfunction syndrome and further classified as either developmental lag (38%) or organic brain syndrome (62%), were followed into late adolescence and early adult life. At follow-up 7% were free of psychiatric disorder, 80% had various types of personality disorder, and 14% were borderline psychotic. Global outcome was rated as satisfactory in 20%, unsatisfactory in 80%. Associated with an unsatisfactory outcome were these initial findings: low normal or borderline intelligence, multiplicity of behavioral and neuropsychological findings, learning disabilities, special class placement, and initial classification of organic brain syndrome. Initial differentiation between developmental lag and organic brain syndrome foreshadowed different psychopathological features having significant polarities and little overlap, as well as different global outcome."}
{"id": "PMID:479114", "title": "Differential diagnosis of fugue-like states.", "content": "Fugue-like states, though usually considered hysterical or epileptic in origin, can occur with many other psychiatric and neurologic conditions. The survey of literature should help the clinician with differential diagnosis. Nine illustrative cases are described and guidelines for investigating a case of fugue are outlined.", "contents": "Differential diagnosis of fugue-like states. Fugue-like states, though usually considered hysterical or epileptic in origin, can occur with many other psychiatric and neurologic conditions. The survey of literature should help the clinician with differential diagnosis. Nine illustrative cases are described and guidelines for investigating a case of fugue are outlined."}
{"id": "PMID:479115", "title": "The clinical utility of reproduction drawing tests with low IQ patients.", "content": "Bedside mental status testing in low IQ, poorly educated patients is often difficult. These patients, who comprise a significant proportion of the population served by major inner city public hospitals, have a limited ability to perform adequately on many higher cortical function tests. Because of this, it is often difficult to accurately differentiate between an organic brain syndrome and the effect of depressed intelligence in these patients. Copying line drawings and other constructional tasks are a very important element in a complete mental status examination, as they are frequently failed by patients with organic brain disease. The applicability of drawing tasks in the low IQ patient is an important issue; this study demonstrates that selected drawings can be effectively utilized to identify brain dysfunction in this low IQ population.", "contents": "The clinical utility of reproduction drawing tests with low IQ patients. Bedside mental status testing in low IQ, poorly educated patients is often difficult. These patients, who comprise a significant proportion of the population served by major inner city public hospitals, have a limited ability to perform adequately on many higher cortical function tests. Because of this, it is often difficult to accurately differentiate between an organic brain syndrome and the effect of depressed intelligence in these patients. Copying line drawings and other constructional tasks are a very important element in a complete mental status examination, as they are frequently failed by patients with organic brain disease. The applicability of drawing tasks in the low IQ patient is an important issue; this study demonstrates that selected drawings can be effectively utilized to identify brain dysfunction in this low IQ population."}
{"id": "PMID:479116", "title": "Cognitive impairment and treatment outcome with alcoholics: preliminary findings.", "content": "The relationship of cognitive impairment to treatment outcome in a sample of male alcoholics (N = 30) was explored. The Brain-Age Quotient (BAQ), an index of problem solving and adaptive abilities based on a battery of neuropsychological tests, was used as the summary measure of cognitive impairment. High BAQ scores were found to be more highly related to clinical ratings of patients' level of functioning on the treatment ward, to the successful completion of inpatient treatment, and to fewer relapses, longer abstinence periods, and lower rates of alcohol consumption at a 1 year follow-up than were lower BAQ scores. The implications of these findings with regard to appropriate treatment planning are discussed.", "contents": "Cognitive impairment and treatment outcome with alcoholics: preliminary findings. The relationship of cognitive impairment to treatment outcome in a sample of male alcoholics (N = 30) was explored. The Brain-Age Quotient (BAQ), an index of problem solving and adaptive abilities based on a battery of neuropsychological tests, was used as the summary measure of cognitive impairment. High BAQ scores were found to be more highly related to clinical ratings of patients' level of functioning on the treatment ward, to the successful completion of inpatient treatment, and to fewer relapses, longer abstinence periods, and lower rates of alcohol consumption at a 1 year follow-up than were lower BAQ scores. The implications of these findings with regard to appropriate treatment planning are discussed."}
{"id": "PMID:479117", "title": "Effects of divalent cations on thermophilic inorganic pyrophosphatase.", "content": "Divalent cations were shown to affect the structure and thermostability of thermophilic inorganic pyrophosphatase [pyrophosphate phosphohydrolase EC 3.6.1.1] purified from Bacillus stearothermophilus and thermophilic bacterium PS-3. The properties of the enzymes from the two sources were found to be very similar. The enzymes were very unstable to heart in the absence of divalent cations, being inactivated gradually even at 40 degrees C. However, they became stable to heat denaturation in the presence of Mg2+, between pH 7.8 and 9.0. Similar induced thermostability was detected when Mn2+, Co2+, Ca2+, Cd2+, and ZN2+ were added, though the latter three cations were not essential for enzyme activity. On adding divalent cations, the optical properties such as absorption spectra, fluorescence spectra, and circular dichroism (CD) were changed. Gel filtration and disc electrophoresis revealed that the molecular weight of both enzymes was 5.4 x 10(4) in Tris-SO4 buffer and 11 x 10(4) in Tris-HCL buffer, suggesting monomer-dimer transformation. In the presence of divalent cations in Tris-SO4 fuffer, the enzymes dimerized; this was confirmed by sedimentation velocity measurements. The enzymes in Tris-HCL buffer did not show thermostability unless divalent cations were added. The results in the present study indicate that binding of divalent cations to each enzyme caused some conformational change in the vicinity of aromatic amino acid residues leading to dimerization of the enzyme molecule so that it became thermostable. It was also suggested that histidyl residues play an important role in the thermostability induced by divalent cations on the basis of the pH dependencies of thermostability and CD spectra.", "contents": "Effects of divalent cations on thermophilic inorganic pyrophosphatase. Divalent cations were shown to affect the structure and thermostability of thermophilic inorganic pyrophosphatase [pyrophosphate phosphohydrolase EC 3.6.1.1] purified from Bacillus stearothermophilus and thermophilic bacterium PS-3. The properties of the enzymes from the two sources were found to be very similar. The enzymes were very unstable to heart in the absence of divalent cations, being inactivated gradually even at 40 degrees C. However, they became stable to heat denaturation in the presence of Mg2+, between pH 7.8 and 9.0. Similar induced thermostability was detected when Mn2+, Co2+, Ca2+, Cd2+, and ZN2+ were added, though the latter three cations were not essential for enzyme activity. On adding divalent cations, the optical properties such as absorption spectra, fluorescence spectra, and circular dichroism (CD) were changed. Gel filtration and disc electrophoresis revealed that the molecular weight of both enzymes was 5.4 x 10(4) in Tris-SO4 buffer and 11 x 10(4) in Tris-HCL buffer, suggesting monomer-dimer transformation. In the presence of divalent cations in Tris-SO4 fuffer, the enzymes dimerized; this was confirmed by sedimentation velocity measurements. The enzymes in Tris-HCL buffer did not show thermostability unless divalent cations were added. The results in the present study indicate that binding of divalent cations to each enzyme caused some conformational change in the vicinity of aromatic amino acid residues leading to dimerization of the enzyme molecule so that it became thermostable. It was also suggested that histidyl residues play an important role in the thermostability induced by divalent cations on the basis of the pH dependencies of thermostability and CD spectra."}
{"id": "PMID:479118", "title": "The site of inhibition of cell wall synthesis by 3-amino-3-deoxy-D-glucose in Staphylococcus aureus.", "content": "The inhibition of growth and cell wall synthesis by 3-amino-3-deoxy-D-glucose (3-AG), which is known to be one of the constituents of the kanamycin molecule and a metabolite of Bacillus sp., was almost completely overcome by glucosamine and N-acetylglucosamine in Staphylococcus aureus but scarcely affected by D-glucose and D-fructose. The antibiotic did not inhibit the incorporation of [14C]glucosamine and [3H]N-acetylglucosamine into the acid-insoluble fraction, but rather enhanced the incorporation of [14C]glucosamine. On the other hand, it inhibited the incorporation of D-[14C]fructose into the cell wall fraction but hardly affected the incorporation of D-[14C]fructose into the acid-insoluble fraction in the presence of pencillin G. Based on these results, it is suggested that the site of primary action of 3-AG is the formation of glucosamine-6-phosphate from D-fructose-6-phosphate, which is catalyzed by glucosamine synthetase [EC 2.6.1.16].", "contents": "The site of inhibition of cell wall synthesis by 3-amino-3-deoxy-D-glucose in Staphylococcus aureus. The inhibition of growth and cell wall synthesis by 3-amino-3-deoxy-D-glucose (3-AG), which is known to be one of the constituents of the kanamycin molecule and a metabolite of Bacillus sp., was almost completely overcome by glucosamine and N-acetylglucosamine in Staphylococcus aureus but scarcely affected by D-glucose and D-fructose. The antibiotic did not inhibit the incorporation of [14C]glucosamine and [3H]N-acetylglucosamine into the acid-insoluble fraction, but rather enhanced the incorporation of [14C]glucosamine. On the other hand, it inhibited the incorporation of D-[14C]fructose into the cell wall fraction but hardly affected the incorporation of D-[14C]fructose into the acid-insoluble fraction in the presence of pencillin G. Based on these results, it is suggested that the site of primary action of 3-AG is the formation of glucosamine-6-phosphate from D-fructose-6-phosphate, which is catalyzed by glucosamine synthetase [EC 2.6.1.16]."}
{"id": "PMID:479119", "title": "Formation of roseoflavin from guanine through riboflavin.", "content": "A synthesis of roseoflavin by Streptomyces davawensis from guanine through riboflavin was demonstrated. The lines of evidence are (1)incorporations of 14C of [2-and U-14C] guanine and [2-14C] riboflavin into roseoflavin, (2) no incorporation of 14C of [8-14C] guanine into roseoflavin, (3) localizations of 14C in roseoflavin, and (4) a decrease of specific radioactivity of roseoflavin formed from [2-14C]guanine on addition of riboflavin to the culture. The 14C atoms in roseoflavin formed were localized by radioactivity analysis of the NaOH-hydrolysis products, i.e., urea and 1,2-dihydro-6-methyl-7-dimethylamino-2-keto-1-D-ribityl-3-quinox-alinecarboxylic acid (QC), a new substance. These hydrolysis products were identified by the isolation of dixanthylures, decomposition with urease, and from  the properties of QC and QC tetraacetate isolated. These finding suggest that the pyrimidine ring of guanine is conserved in the formation of roseoflavin from guanine through riboflavin.", "contents": "Formation of roseoflavin from guanine through riboflavin. A synthesis of roseoflavin by Streptomyces davawensis from guanine through riboflavin was demonstrated. The lines of evidence are (1)incorporations of 14C of [2-and U-14C] guanine and [2-14C] riboflavin into roseoflavin, (2) no incorporation of 14C of [8-14C] guanine into roseoflavin, (3) localizations of 14C in roseoflavin, and (4) a decrease of specific radioactivity of roseoflavin formed from [2-14C]guanine on addition of riboflavin to the culture. The 14C atoms in roseoflavin formed were localized by radioactivity analysis of the NaOH-hydrolysis products, i.e., urea and 1,2-dihydro-6-methyl-7-dimethylamino-2-keto-1-D-ribityl-3-quinox-alinecarboxylic acid (QC), a new substance. These hydrolysis products were identified by the isolation of dixanthylures, decomposition with urease, and from  the properties of QC and QC tetraacetate isolated. These finding suggest that the pyrimidine ring of guanine is conserved in the formation of roseoflavin from guanine through riboflavin."}
{"id": "PMID:479120", "title": "Two components of chorismate mutase in Brevibacterium flavum.", "content": "Chorismate mutase of Brevibacterium flavum, a common enzyme in phenylalanine and tyrosine biosynthesis, was separted into two different component, A and B, with molecular weights of 250,000 and 25,000, respectively, by ammonium sulfate fractionation or gel-filtration. Both components were essential for the enzymatic activity. In the presence of the reaction substrate, chorismate, the two components associated reversibly to give an active enzyme complex with a molecular weight of 320,000. Binding sites of the feedback inhibitors, phenylalanine and tyrosine, on the enzyme were localized on component A as determined by hybridization experiments with the wild-type and mutant components. Tyrosine repressed the synthesis of component B much more strongly than that of component A, while phenylalanine did not show any significant repressive effect on either component. The wild-type strain No. 2247 had four times more component A than component B. Elution patterns in gel, DEAE-cellulose or hydroxyapatite column chromatography as well as the disc-gel electrophoretic pattern of chorismate mutase component A and 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase activities completely overlapped, suggesting the presence of a bifunctional protein having the two activities. In accord with this suggestion, chorismate mutase as well as DAHP synthetase was insensitive to feedback inhibition by phenylalanine and tyrosine in all the 3-fluorophenylalanine-resistant mutants tested that excreted both phenylalanine and tyrosine. All the phenylalanine and tyrosine double auxotrophs defective in chorismate mutase lacked component B but not A.", "contents": "Two components of chorismate mutase in Brevibacterium flavum. Chorismate mutase of Brevibacterium flavum, a common enzyme in phenylalanine and tyrosine biosynthesis, was separted into two different component, A and B, with molecular weights of 250,000 and 25,000, respectively, by ammonium sulfate fractionation or gel-filtration. Both components were essential for the enzymatic activity. In the presence of the reaction substrate, chorismate, the two components associated reversibly to give an active enzyme complex with a molecular weight of 320,000. Binding sites of the feedback inhibitors, phenylalanine and tyrosine, on the enzyme were localized on component A as determined by hybridization experiments with the wild-type and mutant components. Tyrosine repressed the synthesis of component B much more strongly than that of component A, while phenylalanine did not show any significant repressive effect on either component. The wild-type strain No. 2247 had four times more component A than component B. Elution patterns in gel, DEAE-cellulose or hydroxyapatite column chromatography as well as the disc-gel electrophoretic pattern of chorismate mutase component A and 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase activities completely overlapped, suggesting the presence of a bifunctional protein having the two activities. In accord with this suggestion, chorismate mutase as well as DAHP synthetase was insensitive to feedback inhibition by phenylalanine and tyrosine in all the 3-fluorophenylalanine-resistant mutants tested that excreted both phenylalanine and tyrosine. All the phenylalanine and tyrosine double auxotrophs defective in chorismate mutase lacked component B but not A."}
{"id": "PMID:479121", "title": "Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bovine serum albumin oligomers produced by lipid peroxidation.", "content": "The oligomers of bovine serum albumin were produced by controlled reaction with peroxidizing linoleic acid to examine their possible utility as calibration proteins insodium dodecyl sulfate-polyacrylamide gel electrophoresis. The polymerization was effected in reaction mixtures containing linoleic acid  undergoing peroxidation in the presence of ascorbic acid, and conditions that yield soluble oligomers with a wide molecular weight distribution were established. The interaction of these soluble oligomers with sodium dodecyl sulfate exhibited a binding isotherm indistinguishable from that obtained with bovine serum albumin. Furthermore, sodium dodecy sulfate-polyacrylamide gel electrophoresis of the albumin oligomers conformed to the empirical relation of molecular weight to mobility that pertains to the use of these oligomers as standard molecular weight markers.", "contents": "Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bovine serum albumin oligomers produced by lipid peroxidation. The oligomers of bovine serum albumin were produced by controlled reaction with peroxidizing linoleic acid to examine their possible utility as calibration proteins insodium dodecyl sulfate-polyacrylamide gel electrophoresis. The polymerization was effected in reaction mixtures containing linoleic acid  undergoing peroxidation in the presence of ascorbic acid, and conditions that yield soluble oligomers with a wide molecular weight distribution were established. The interaction of these soluble oligomers with sodium dodecyl sulfate exhibited a binding isotherm indistinguishable from that obtained with bovine serum albumin. Furthermore, sodium dodecy sulfate-polyacrylamide gel electrophoresis of the albumin oligomers conformed to the empirical relation of molecular weight to mobility that pertains to the use of these oligomers as standard molecular weight markers."}
{"id": "PMID:479122", "title": "Effect of diaminobutene and diaminopropane on diet-stimulated polyamine synthesis and cell proliferation in rat liver.", "content": "The effect of two putrescine analogs were studied on hepatic polyamine synthesis and cell proliferation, both of which were stimulated by food intake. Trans-1, 4-diamino-2-butene (diaminobutene), which is a potent competitive inhibitor of ornithine decarboxylase [EC 4.1.1.17] (ODC), repressed the induction of ODC and effectively inhibited the accumulation of putrescine in rat liver which was induced by the feeding of dietary protein. Unexpectedly, diaminobutene did not suppress DNA synthesis and mitotic activity in rat liver, suggesting that it can mimic the role of putrescine in cell proliferation. 1,3-Diaminopropane effectively repressed the induction of ODC caused by food intake and also suppressed DNA synthesis and mitotic activity without affecting the accumulation of RNA or protein. The suppression of mitotic activity by 1,3-diaminopropane was reversed by a single injection of putrescine, spermidine, spermine, or diaminobutene. It was concluded that rapid accumulation of polyamines, especially putrescine, was a prerequisite for the later enhancement of DNA synthesis and cell proliferation in rat liver caused by food intake.", "contents": "Effect of diaminobutene and diaminopropane on diet-stimulated polyamine synthesis and cell proliferation in rat liver. The effect of two putrescine analogs were studied on hepatic polyamine synthesis and cell proliferation, both of which were stimulated by food intake. Trans-1, 4-diamino-2-butene (diaminobutene), which is a potent competitive inhibitor of ornithine decarboxylase [EC 4.1.1.17] (ODC), repressed the induction of ODC and effectively inhibited the accumulation of putrescine in rat liver which was induced by the feeding of dietary protein. Unexpectedly, diaminobutene did not suppress DNA synthesis and mitotic activity in rat liver, suggesting that it can mimic the role of putrescine in cell proliferation. 1,3-Diaminopropane effectively repressed the induction of ODC caused by food intake and also suppressed DNA synthesis and mitotic activity without affecting the accumulation of RNA or protein. The suppression of mitotic activity by 1,3-diaminopropane was reversed by a single injection of putrescine, spermidine, spermine, or diaminobutene. It was concluded that rapid accumulation of polyamines, especially putrescine, was a prerequisite for the later enhancement of DNA synthesis and cell proliferation in rat liver caused by food intake."}
{"id": "PMID:479123", "title": "Process of iodination of thyroglobulin and its maturation. II. Properties and distribution of thyroglobulin labeled in vitro or in vivo with radioiodine, 3H-tyrosine, or 3H-galactose in rat thyroid glands.", "content": "With the aim of obtaining information on the process of iodination of thyroglobulin, the properties and subcellular distribution of thyroglobulin labeled with radioiodine, 3H-tyrosine, or 3H-galactose were studied. The following results were obtained for 17-19S thyroglobulin isolated from rat thyroid lobes labeled in vitro. (a) The effect of sodium dodecyl sulfate (SDS) concentration (0.1-2.0 mM) on the dissociability of the proteins into 12S subunits showed that 3H-labeled, 131I-labeled, and preformed thyroglobulin behaved very differently; their dissociability decreased in that order. In addition, 0.3 mM SDS is most suitable for discriminating among these species. (b) The amount of 0.3 mM SDS-resistant 131I-thyroglobulin increased with the time of incubation of the lobes or with the amount of iodine atoms incorporated by chemical iodination. (c) Digestion of 3H-tyrosine-labeled thyroglobulin showed that 3H-monoiodotyrosine and 3H-diiodotyrosine were present after incubation of the lobes for 180 min. (d) The dissociability of 3H-galactose-labeled 17-19S thyroglobulin was higher than that of 131I-labeled protein, but its elution pattern on DEAE-cellulose chromatography resembled that of the latter. (e) 131I-Thyroglobulin was scarcely found in the incubation medium, although a considerable amount of 19S thyroglobulin was released into the medium during the incubation. As for the lobes, a significant amount of 131I-radioactivity as well as 3H-radioactivity was found in cytoplasmic particulates, especially in fractions containing apical vesicles and rough microsomes. On the other hand, the following results were obtained for 17-19S thyroglobulin isolated from rats injected with 125I. (a) Dissociability of the protein by 0.3 mM SDS and analysis of 125I-iodoamino acids of pronase digest showed that the iodination process was essentially similar to the case of in vitro incorporation, but was faster. (b) The effect of cyclohiximide treatment showed that the relative reduction of 0.3 mM SDS dissociable species was probably due to a shortage of newly synthesized proteins. All the results obtained in the present experiments are compatible with the view that iodine atoms are incorporated selectively into newly synthesized, less iodinated thyroglobulin, and that the iodination occurs intracellularly, at least to a certain degree, after carbohydrate attachment, probably in the apical vesicles. The possibility that iodination also occurs to some extent in the endoplasmic reticulum and in the colloid lumen of thyroglobulin-stimulated thyroids is discussed.", "contents": "Process of iodination of thyroglobulin and its maturation. II. Properties and distribution of thyroglobulin labeled in vitro or in vivo with radioiodine, 3H-tyrosine, or 3H-galactose in rat thyroid glands. With the aim of obtaining information on the process of iodination of thyroglobulin, the properties and subcellular distribution of thyroglobulin labeled with radioiodine, 3H-tyrosine, or 3H-galactose were studied. The following results were obtained for 17-19S thyroglobulin isolated from rat thyroid lobes labeled in vitro. (a) The effect of sodium dodecyl sulfate (SDS) concentration (0.1-2.0 mM) on the dissociability of the proteins into 12S subunits showed that 3H-labeled, 131I-labeled, and preformed thyroglobulin behaved very differently; their dissociability decreased in that order. In addition, 0.3 mM SDS is most suitable for discriminating among these species. (b) The amount of 0.3 mM SDS-resistant 131I-thyroglobulin increased with the time of incubation of the lobes or with the amount of iodine atoms incorporated by chemical iodination. (c) Digestion of 3H-tyrosine-labeled thyroglobulin showed that 3H-monoiodotyrosine and 3H-diiodotyrosine were present after incubation of the lobes for 180 min. (d) The dissociability of 3H-galactose-labeled 17-19S thyroglobulin was higher than that of 131I-labeled protein, but its elution pattern on DEAE-cellulose chromatography resembled that of the latter. (e) 131I-Thyroglobulin was scarcely found in the incubation medium, although a considerable amount of 19S thyroglobulin was released into the medium during the incubation. As for the lobes, a significant amount of 131I-radioactivity as well as 3H-radioactivity was found in cytoplasmic particulates, especially in fractions containing apical vesicles and rough microsomes. On the other hand, the following results were obtained for 17-19S thyroglobulin isolated from rats injected with 125I. (a) Dissociability of the protein by 0.3 mM SDS and analysis of 125I-iodoamino acids of pronase digest showed that the iodination process was essentially similar to the case of in vitro incorporation, but was faster. (b) The effect of cyclohiximide treatment showed that the relative reduction of 0.3 mM SDS dissociable species was probably due to a shortage of newly synthesized proteins. All the results obtained in the present experiments are compatible with the view that iodine atoms are incorporated selectively into newly synthesized, less iodinated thyroglobulin, and that the iodination occurs intracellularly, at least to a certain degree, after carbohydrate attachment, probably in the apical vesicles. The possibility that iodination also occurs to some extent in the endoplasmic reticulum and in the colloid lumen of thyroglobulin-stimulated thyroids is discussed."}
{"id": "PMID:479124", "title": "Crystallization and preliminary crystallographic data of chicken gizzard G-actin . DNase I complex and Physarum G-actin . DNase I complex.", "content": "Smooth muscle G-actin from chicken gizzard and Physarum plasmodium G-actin both interact with DNase I and form 1 : 1 complexes. These complexes were crystallized by using polyethylene glycol 6000 as a precipitant. Both crystals belong to the same orthorhombic space group P2(1)2(1)2(1). The cell dimensions of chicken gizzard G-actin.DNase I complex are a=42.00 +/- 0.07 A, b=225.3 +/- 0.4 A, and c=77.4 +/- 0.1 A, while those of Physarum G-actin.DNase I complex are a=42 A, b=221 A, and c=77 A.", "contents": "Crystallization and preliminary crystallographic data of chicken gizzard G-actin . DNase I complex and Physarum G-actin . DNase I complex. Smooth muscle G-actin from chicken gizzard and Physarum plasmodium G-actin both interact with DNase I and form 1 : 1 complexes. These complexes were crystallized by using polyethylene glycol 6000 as a precipitant. Both crystals belong to the same orthorhombic space group P2(1)2(1)2(1). The cell dimensions of chicken gizzard G-actin.DNase I complex are a=42.00 +/- 0.07 A, b=225.3 +/- 0.4 A, and c=77.4 +/- 0.1 A, while those of Physarum G-actin.DNase I complex are a=42 A, b=221 A, and c=77 A."}
{"id": "PMID:479125", "title": "Cerebroside sulfotransferase activity in human lung tissues. An elevated level in lung adenocarcinoma.", "content": "Cerebroside sulfotransferase activity was demonstrated in particulate fractions from human lung and its carcinoma tissues. The activity in human lung adenocarcinoma was significantly higher than those in a different histological type of carcinoma (squamous cell carcinoma) and in normal tissue from which each carcinoma was derived.", "contents": "Cerebroside sulfotransferase activity in human lung tissues. An elevated level in lung adenocarcinoma. Cerebroside sulfotransferase activity was demonstrated in particulate fractions from human lung and its carcinoma tissues. The activity in human lung adenocarcinoma was significantly higher than those in a different histological type of carcinoma (squamous cell carcinoma) and in normal tissue from which each carcinoma was derived."}
{"id": "PMID:479126", "title": "Crystallographic data for cytochrome c3 from two strains of Desulfovibrio vulgaris, Miyazaki.", "content": "Two crystalline forms of cytochrome c3 isolated from two strains of Desulfovibrio vulgaris, Miyazaki, tentatively designated as D. vulgaris, Miyazki F and  D. vulgaris, Miyazaki K, have been found. Both belong to the orthorhombic system, space group P2(1)2(1)2(1), but have different cell dimensions; a=54.1, b=68.9 and c=35.0 A for D. vulgaris, Miyazaki F, and a=43.5, b=41.2, and c=62.9 A for D. vulgaris, Miyazaki K. The asymmetric unit of each crystal contains one molecule of cytochrome c3.", "contents": "Crystallographic data for cytochrome c3 from two strains of Desulfovibrio vulgaris, Miyazaki. Two crystalline forms of cytochrome c3 isolated from two strains of Desulfovibrio vulgaris, Miyazaki, tentatively designated as D. vulgaris, Miyazki F and  D. vulgaris, Miyazaki K, have been found. Both belong to the orthorhombic system, space group P2(1)2(1)2(1), but have different cell dimensions; a=54.1, b=68.9 and c=35.0 A for D. vulgaris, Miyazaki F, and a=43.5, b=41.2, and c=62.9 A for D. vulgaris, Miyazaki K. The asymmetric unit of each crystal contains one molecule of cytochrome c3."}
{"id": "PMID:479127", "title": "Catalytic activity of the chromophore of desulfoviridin, sirohydrochlorin, in sulfite reduction in the presence of iron.", "content": "Sirohydrochlorin chromophore prepared by acetone/HCl treatment of desulfoviridin, the sulfite reductase from Desulfovibrio, catalyzed the reduction of sulfite to sulfide and thiosulfate in equimolar amounts when coupled with a hydrogen-hydrogenase-methyl viologen system. This activity was manifested at acidic pH and increased exponentially with decrease in pH. The Km value for sulfite was nearly 10 times that of desulfoviridin. Inorganic iron was necessary for the reduction, since inactivation occurred on passage through a Sephadex LH-20 column or in the presence of 2,2'-bipyridine, and reactivation was observed on adding iron. The chromophore catalyzed the reduction of dithionite and hyroxylamine.", "contents": "Catalytic activity of the chromophore of desulfoviridin, sirohydrochlorin, in sulfite reduction in the presence of iron. Sirohydrochlorin chromophore prepared by acetone/HCl treatment of desulfoviridin, the sulfite reductase from Desulfovibrio, catalyzed the reduction of sulfite to sulfide and thiosulfate in equimolar amounts when coupled with a hydrogen-hydrogenase-methyl viologen system. This activity was manifested at acidic pH and increased exponentially with decrease in pH. The Km value for sulfite was nearly 10 times that of desulfoviridin. Inorganic iron was necessary for the reduction, since inactivation occurred on passage through a Sephadex LH-20 column or in the presence of 2,2'-bipyridine, and reactivation was observed on adding iron. The chromophore catalyzed the reduction of dithionite and hyroxylamine."}
{"id": "PMID:479128", "title": "On the formation of gamma-aminobutyric acid from putrescine in brain.", "content": "Gamma-aminobutyric acid is not formed in significant amounts from putrescine by incubation with rat brain homogenates. However, it is formed if acetyl-CoA is added to the incubation medium. This is taken as further evidence for the existence of a metabolic pathway in mammalian brain which comprises acetylation of putrescine to monoacetyl putrescine and oxidative deamination of monoacetyl putrescine by MAO. Nerve cells and glia cells have comparable capacities for putrescine degradation along this pathway.", "contents": "On the formation of gamma-aminobutyric acid from putrescine in brain. Gamma-aminobutyric acid is not formed in significant amounts from putrescine by incubation with rat brain homogenates. However, it is formed if acetyl-CoA is added to the incubation medium. This is taken as further evidence for the existence of a metabolic pathway in mammalian brain which comprises acetylation of putrescine to monoacetyl putrescine and oxidative deamination of monoacetyl putrescine by MAO. Nerve cells and glia cells have comparable capacities for putrescine degradation along this pathway."}
{"id": "PMID:479129", "title": "Carboxymethylation of a minor ribonuclease from Aspergillus saitoi.", "content": "(1) RNase Ms was inactivated by iodoacetate. The inactivation was most rapid at pH 6.0, and was inhibited in the presence of a denaturant such as 8 m urea or 6 m guanidine-HCL. (2) Competitive inhibitors protected RNase Ms from inactivation by iodoacetate; the effect was in the order 2',(3')-GTP greater than 2',(3')-AMP, 2',(3')-UMP greater than or equal to 2',(3')-CMP. The order is not consistent with that of the binding constants of the 4 nucleotides towards RNase Ms (A is greater than C greater than G greater than U). (3) RNase Ms was inactivated with the concomitant incorporation of one molar equivalent of carboxymethly group. The following evidence indicated that the carboxymethyl group was incorporated into the carboxyl group of an aspartic acid or glutamic acid residue. (i) The carboxymethyl group incorporated into RNase Ms was liberated by treatment with 0.1 n NaOH or 1 m hydroxylamine. (ii) The amino acid composition of carboxymethylated RNase Ms (CM RNase Ms) after acid hydrolysis is similar to that of RNase Ms. (4) 14C-Labeled CM RNase Ms was digested successively with alkaline protease and amino-peptidase M. The radioactive amino acid released was eluted just before aspartate on an amino acid analyzer. After hydrolysis with 6 n HCL, glutamic acid was produced exclusively from the radioactive amino acid. The specific radioactivity of this amino acid calculated from the radioactivity and glutamic acid formed was practctically the same as that of CM RNase Ms. Thus, it was concluded that a carboxymethyl group was incorporated at the carboxyl group of a glutamic acid residue of RNnase Ms. (5) CM RNase Ms bound with 2'-AMP to the same extent as native RNase Ms, but bound to a lesser extent with 2',(3')-GMP. (6) Although the conformation of CM RNase Ms as judged from the CD spectrum was practically the same as that of native RNase Ms, the reactivity of CM RNase Ms towards dinitrofluorobenzene was different from that of native RNase Ms, indicating some difference in the conformation. (7) These results indicate that one glutamic acid residue is involved in the active of RNase Ms.", "contents": "Carboxymethylation of a minor ribonuclease from Aspergillus saitoi. (1) RNase Ms was inactivated by iodoacetate. The inactivation was most rapid at pH 6.0, and was inhibited in the presence of a denaturant such as 8 m urea or 6 m guanidine-HCL. (2) Competitive inhibitors protected RNase Ms from inactivation by iodoacetate; the effect was in the order 2',(3')-GTP greater than 2',(3')-AMP, 2',(3')-UMP greater than or equal to 2',(3')-CMP. The order is not consistent with that of the binding constants of the 4 nucleotides towards RNase Ms (A is greater than C greater than G greater than U). (3) RNase Ms was inactivated with the concomitant incorporation of one molar equivalent of carboxymethly group. The following evidence indicated that the carboxymethyl group was incorporated into the carboxyl group of an aspartic acid or glutamic acid residue. (i) The carboxymethyl group incorporated into RNase Ms was liberated by treatment with 0.1 n NaOH or 1 m hydroxylamine. (ii) The amino acid composition of carboxymethylated RNase Ms (CM RNase Ms) after acid hydrolysis is similar to that of RNase Ms. (4) 14C-Labeled CM RNase Ms was digested successively with alkaline protease and amino-peptidase M. The radioactive amino acid released was eluted just before aspartate on an amino acid analyzer. After hydrolysis with 6 n HCL, glutamic acid was produced exclusively from the radioactive amino acid. The specific radioactivity of this amino acid calculated from the radioactivity and glutamic acid formed was practctically the same as that of CM RNase Ms. Thus, it was concluded that a carboxymethyl group was incorporated at the carboxyl group of a glutamic acid residue of RNnase Ms. (5) CM RNase Ms bound with 2'-AMP to the same extent as native RNase Ms, but bound to a lesser extent with 2',(3')-GMP. (6) Although the conformation of CM RNase Ms as judged from the CD spectrum was practically the same as that of native RNase Ms, the reactivity of CM RNase Ms towards dinitrofluorobenzene was different from that of native RNase Ms, indicating some difference in the conformation. (7) These results indicate that one glutamic acid residue is involved in the active of RNase Ms."}
{"id": "PMID:479130", "title": "Purification and properties of xanthine dehydrogenase from Streptomyces cyanogenus.", "content": "Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 457 nm and contains FAD, iron, and labile sulfide in a molar ratio of 1 : 7 : 1 per subunit. Little molybdenum could be detected. The enzyme is most active at pH 8.7 and at 40 degrees C, and is stable between pH 7 and 12 (at 4 degrees C for 24 h) and below 55 degrees C (at pH 9 for 10 min). The activity is stimulated by K+ at a concentration of 50 mM or more and also by keeping the enzyme at pH 9 to 11. The activity is inhibited by cyanide, Tiron, and p-chloromercuribenzoate and by adenine and urate. Among the compounds tested, hypoxanthine, guanine, xanthine 2-hydroxypurine, and 6,8-dihydroxypurine are oxidized at considerable rates; hypoxanthine is the best substrate. NAD+ is the preferred electron acceptor. Km values of the enzyme for hypoxanthine, guanine, xanthine, and NAD+ are 0.055, 0.015, 0.15, and 0.11 mM, respectively. Marked differences in the properties of this enzyme compared to others are the activity towards guanine, which has a higher affinity for the enzyme than hypoxanthine and xanthine, and a higher reactivity with hypoxanthine than xanthine. The organism has been identified as Streptomyces cyanogenus.", "contents": "Purification and properties of xanthine dehydrogenase from Streptomyces cyanogenus. Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 457 nm and contains FAD, iron, and labile sulfide in a molar ratio of 1 : 7 : 1 per subunit. Little molybdenum could be detected. The enzyme is most active at pH 8.7 and at 40 degrees C, and is stable between pH 7 and 12 (at 4 degrees C for 24 h) and below 55 degrees C (at pH 9 for 10 min). The activity is stimulated by K+ at a concentration of 50 mM or more and also by keeping the enzyme at pH 9 to 11. The activity is inhibited by cyanide, Tiron, and p-chloromercuribenzoate and by adenine and urate. Among the compounds tested, hypoxanthine, guanine, xanthine 2-hydroxypurine, and 6,8-dihydroxypurine are oxidized at considerable rates; hypoxanthine is the best substrate. NAD+ is the preferred electron acceptor. Km values of the enzyme for hypoxanthine, guanine, xanthine, and NAD+ are 0.055, 0.015, 0.15, and 0.11 mM, respectively. Marked differences in the properties of this enzyme compared to others are the activity towards guanine, which has a higher affinity for the enzyme than hypoxanthine and xanthine, and a higher reactivity with hypoxanthine than xanthine. The organism has been identified as Streptomyces cyanogenus."}
{"id": "PMID:479131", "title": "Isolation and amino acid sequences of proline-rich peptides of human whole saliva.", "content": "Three basic peptides with extremely high proline contents were isolated from human whole saliva. The amino acid sequences of two of these proline-rich peptides comprising 57 and 38 residues were determined by conventional methods. The sequence suggested that the smaller peptide was derived from the larger one and also revealed the occurrence of characteristic repeating units within the molecules. The present study is the first to describe this structural feature of proline-rich proteins or peptides.", "contents": "Isolation and amino acid sequences of proline-rich peptides of human whole saliva. Three basic peptides with extremely high proline contents were isolated from human whole saliva. The amino acid sequences of two of these proline-rich peptides comprising 57 and 38 residues were determined by conventional methods. The sequence suggested that the smaller peptide was derived from the larger one and also revealed the occurrence of characteristic repeating units within the molecules. The present study is the first to describe this structural feature of proline-rich proteins or peptides."}
{"id": "PMID:479132", "title": "A determination of H2O2 release by the treatment of human blood polymorphonuclear leukocytes with myristate.", "content": "Free H2O2 released from human blood leukocytes during phagocytosis into the extracellular medium was highly reactive with the ferric form of HRP, forming an enzyme-substrate complex which was identical to HRP-H2O2 compound II. The formation of HRP-H2O2 compound II was employed for assaying the rates of H2O2 release by leukocytes upon addition of bacteria or myristate. The treatment of normal human blood leukocytes with myristate resulted in a marked stimulation of H2O2 release compared to phagocytizing cells. The activity of H2O2 release in response to myristate was found to be deficient in the leukocytes of two patients with chronic granulomatous disease. This assay method with myristate supplementation is so sensitive and specific that it should be useful for the diagnosis of chronic granulomatous disease.", "contents": "A determination of H2O2 release by the treatment of human blood polymorphonuclear leukocytes with myristate. Free H2O2 released from human blood leukocytes during phagocytosis into the extracellular medium was highly reactive with the ferric form of HRP, forming an enzyme-substrate complex which was identical to HRP-H2O2 compound II. The formation of HRP-H2O2 compound II was employed for assaying the rates of H2O2 release by leukocytes upon addition of bacteria or myristate. The treatment of normal human blood leukocytes with myristate resulted in a marked stimulation of H2O2 release compared to phagocytizing cells. The activity of H2O2 release in response to myristate was found to be deficient in the leukocytes of two patients with chronic granulomatous disease. This assay method with myristate supplementation is so sensitive and specific that it should be useful for the diagnosis of chronic granulomatous disease."}
{"id": "PMID:479133", "title": "Substrate specificity and reaction mechanism of putrescine oxidase.", "content": "Putrescine oxidase [EC 1.4.3.4] of Micrococcus rubens oxidizes many kinds of synthetic polyamines: triamines (spermidine types), tetramines (spermine types), and N-substituted putrescines. Polyamines possessing terminal 4-aminobutylimino groups in their structures were more active as substrates. Putreanine was oxidized at a rate comparable to that of putrescine, and was converted to 1-pyrroline and beta=alanine. Activities and Km values for polyamines were affected by the substituent attached to the 4-aminobutylimino group of the polyamine, and especially by its methylene chain length. It was also found that two types of oxidation occurred in the oxidation of polyamines by putrescine oxidase. When the moieties attached to the 4-aminobutylimino groups in polyamines were less hydrophobic, these polyamines were oxidized at the secondary amino groups to form 1-pyrroline. Polyamines which contained a hydrophobic substituent attached to the 4-aminobutylimino moiety to form ammonia. N,N'-Bis (4-aminobutyl)-1,3-diaminopropane ([II, 4-3-4]) and N-(4-aminobutyl)-N'-(3-aminopropyl)-1,3-diaminopropane ([II, 4-3-3]) were oxidized to form 1-pyrrolinium salt derivatives as a result of oxidation of the terminal primary amino groups. It was concluded that the essential structure for substrates of putrescine oxidase is a 4-aminobutylimino group (NH2(CH2)4NH-).", "contents": "Substrate specificity and reaction mechanism of putrescine oxidase. Putrescine oxidase [EC 1.4.3.4] of Micrococcus rubens oxidizes many kinds of synthetic polyamines: triamines (spermidine types), tetramines (spermine types), and N-substituted putrescines. Polyamines possessing terminal 4-aminobutylimino groups in their structures were more active as substrates. Putreanine was oxidized at a rate comparable to that of putrescine, and was converted to 1-pyrroline and beta=alanine. Activities and Km values for polyamines were affected by the substituent attached to the 4-aminobutylimino group of the polyamine, and especially by its methylene chain length. It was also found that two types of oxidation occurred in the oxidation of polyamines by putrescine oxidase. When the moieties attached to the 4-aminobutylimino groups in polyamines were less hydrophobic, these polyamines were oxidized at the secondary amino groups to form 1-pyrroline. Polyamines which contained a hydrophobic substituent attached to the 4-aminobutylimino moiety to form ammonia. N,N'-Bis (4-aminobutyl)-1,3-diaminopropane ([II, 4-3-4]) and N-(4-aminobutyl)-N'-(3-aminopropyl)-1,3-diaminopropane ([II, 4-3-3]) were oxidized to form 1-pyrrolinium salt derivatives as a result of oxidation of the terminal primary amino groups. It was concluded that the essential structure for substrates of putrescine oxidase is a 4-aminobutylimino group (NH2(CH2)4NH-)."}
{"id": "PMID:479134", "title": "Occurrence of free ceramides in Bacteroides fragilis NCTC 9343.", "content": "The occurrence of free ceramides at high concentrations was demonstrated in the chloroform-methanol extractable lipids of Bacteroides fragilis NCTC 9343. The long-chain bases were isolated from the free ceramides and identified as branched and normal saturated dihydroxy bases with carbon chains consisting of 17, 18, and 19 atoms. The major fatty acid was 3-hydroxy 15-methylhexadecanoic acid. The major molecular species of the ceramides were identified by gas chromatography-mass spectrometry and gas chromatography of the cleaved products as LCB-d-iso17: 0-3-OH iso17: 0 FA, LCB-d-anteiso17: 0-3-OH iso17: 0 FA, LCB-d-iso18: 0-3-OH iso17: 0 FA, and LCB-d-anteiso19: 0-3-OH iso17: 0 FA.", "contents": "Occurrence of free ceramides in Bacteroides fragilis NCTC 9343. The occurrence of free ceramides at high concentrations was demonstrated in the chloroform-methanol extractable lipids of Bacteroides fragilis NCTC 9343. The long-chain bases were isolated from the free ceramides and identified as branched and normal saturated dihydroxy bases with carbon chains consisting of 17, 18, and 19 atoms. The major fatty acid was 3-hydroxy 15-methylhexadecanoic acid. The major molecular species of the ceramides were identified by gas chromatography-mass spectrometry and gas chromatography of the cleaved products as LCB-d-iso17: 0-3-OH iso17: 0 FA, LCB-d-anteiso17: 0-3-OH iso17: 0 FA, LCB-d-iso18: 0-3-OH iso17: 0 FA, and LCB-d-anteiso19: 0-3-OH iso17: 0 FA."}
{"id": "PMID:479135", "title": "Characteristics of D-leucine uptake by mouse Ehrlich ascites tumor cells.", "content": "In a previous paper, we reported that various D-amino acids were taken up several times more effectively than the corresponding L-isomers into several tumors tested in vivo. In order to investigate this further, the in vitro uptake of D-[14C]leucine by Ehrlich ascites tumor cells was investigated in comparison with that of L-[14C]leucine. The distribution ratio, the effects of amino acids and pH, and an approximately linear Lineweaver-Burk plot indicated that D-leucine was transported by an active transport system for L-leucine. Vmax and ku, the first-order rate constant for the unsaturable component, for the uptake of D- and L-leucines decreased with a fall in temperature. The activation energies for Vmax and ku were in the range of 5-10 and 18-21 kcal/mol, respectively. The values for L-leucine were greater than those for D-leucine. Km for D-leucine transport increased with decreasing temperature, whereas Km for L-leucine decreased. This difference suggests that the large alkyl chains of D- and L-leucines bind to different portions of a carrier protein.", "contents": "Characteristics of D-leucine uptake by mouse Ehrlich ascites tumor cells. In a previous paper, we reported that various D-amino acids were taken up several times more effectively than the corresponding L-isomers into several tumors tested in vivo. In order to investigate this further, the in vitro uptake of D-[14C]leucine by Ehrlich ascites tumor cells was investigated in comparison with that of L-[14C]leucine. The distribution ratio, the effects of amino acids and pH, and an approximately linear Lineweaver-Burk plot indicated that D-leucine was transported by an active transport system for L-leucine. Vmax and ku, the first-order rate constant for the unsaturable component, for the uptake of D- and L-leucines decreased with a fall in temperature. The activation energies for Vmax and ku were in the range of 5-10 and 18-21 kcal/mol, respectively. The values for L-leucine were greater than those for D-leucine. Km for D-leucine transport increased with decreasing temperature, whereas Km for L-leucine decreased. This difference suggests that the large alkyl chains of D- and L-leucines bind to different portions of a carrier protein."}
{"id": "PMID:479136", "title": "Age-dependent constitution of chondroitin sulfate isomers in cartilage proteoglycans under associative conditions.", "content": "Associated proteoglycans were prepared with guanidine-HCl from bovine articular cartilage of various ages. They were purified and fractionated by equilibrium centrifugation in cesium chloride (CsCl) density gradients. The compositions of chondroitin sulfate (CS) isomers in associated proteoglycans of articular cartilages of three ages were compared based on the relative amounts of disaccharide units. The results indicated that the proportions of 4-sulfated disaccharide units comprised around 2/3, 1/3, and 1/6 of the total CS in the associated proteoglycans of calf, 18-month-old cow, and 8-year-old cow, respectively. In contrast, the proportions of 6-sulfated disaccharide units in the proteoglycans were in the reverse order; they comprised nearly 1/3, 1/2, and 2/3 of the total CS, respectively, at the three ages. Thus, with increasing age, the ratio of 4-sulfated disaccharide units to 6-sulfated disaccharide units decreased significantly. With the decrease of CsCl density in the gradients, the proportion of 4-sulfated disaccharide units to total CS as well as that of 4-sulfated disaccharide units to 6-sulfated disaccharide units increased in the associated proteoglycans of all ages. The increased ratios of 4-sulfated to 6-sulfated disaccharide units with decreasing CsCl density were significant among the individual proteoglycans: 1.84-2.36 in calf, 0.40-0.89 in 18-month-old cow, and 0.16-0.28 in 8-year-old cow.", "contents": "Age-dependent constitution of chondroitin sulfate isomers in cartilage proteoglycans under associative conditions. Associated proteoglycans were prepared with guanidine-HCl from bovine articular cartilage of various ages. They were purified and fractionated by equilibrium centrifugation in cesium chloride (CsCl) density gradients. The compositions of chondroitin sulfate (CS) isomers in associated proteoglycans of articular cartilages of three ages were compared based on the relative amounts of disaccharide units. The results indicated that the proportions of 4-sulfated disaccharide units comprised around 2/3, 1/3, and 1/6 of the total CS in the associated proteoglycans of calf, 18-month-old cow, and 8-year-old cow, respectively. In contrast, the proportions of 6-sulfated disaccharide units in the proteoglycans were in the reverse order; they comprised nearly 1/3, 1/2, and 2/3 of the total CS, respectively, at the three ages. Thus, with increasing age, the ratio of 4-sulfated disaccharide units to 6-sulfated disaccharide units decreased significantly. With the decrease of CsCl density in the gradients, the proportion of 4-sulfated disaccharide units to total CS as well as that of 4-sulfated disaccharide units to 6-sulfated disaccharide units increased in the associated proteoglycans of all ages. The increased ratios of 4-sulfated to 6-sulfated disaccharide units with decreasing CsCl density were significant among the individual proteoglycans: 1.84-2.36 in calf, 0.40-0.89 in 18-month-old cow, and 0.16-0.28 in 8-year-old cow."}
{"id": "PMID:479137", "title": "In vivo degradation of rat globin messenger RNA during maturation of reticulocytes.", "content": "The degradation of globin mRNA in rat reticulocytes maturing in the peripheral blood was investigated. Poly(A) and non poly(A) portions of mRNA molecules were determined quantitatively by hybridization with radioactive poly(U) and complementary DNA, respectively. During the degradation of mRNA in vivo, it was shown that (1) globin mRNA and the bulk of RNA decrease in parallel, (2) the average chain length of poly(A) segments in the mRNA does not change, (3) the percentage of poly(A) (-) globin mRNA in total globin mRNA does not change, and (4) fragments of large molecular weight do not accumulate. Possible mechanisms of degradation of globin mRNA in the reticulocytes are discussed on the basis of these observations.", "contents": "In vivo degradation of rat globin messenger RNA during maturation of reticulocytes. The degradation of globin mRNA in rat reticulocytes maturing in the peripheral blood was investigated. Poly(A) and non poly(A) portions of mRNA molecules were determined quantitatively by hybridization with radioactive poly(U) and complementary DNA, respectively. During the degradation of mRNA in vivo, it was shown that (1) globin mRNA and the bulk of RNA decrease in parallel, (2) the average chain length of poly(A) segments in the mRNA does not change, (3) the percentage of poly(A) (-) globin mRNA in total globin mRNA does not change, and (4) fragments of large molecular weight do not accumulate. Possible mechanisms of degradation of globin mRNA in the reticulocytes are discussed on the basis of these observations."}
{"id": "PMID:479138", "title": "Separation of the alpha chains of type I and III collagens by SDS-polyacrylamide gel electrophoresis.", "content": "A method for the separation of type III collagen from type I collagen by SDS-polyacrylamide gel electrophoresis has been developed. This is based on the observation that the presence of 3-4 M urea decreases the mobility of the alpha 1 [III] chain to a greater extent than those of the alpha 1[I] and alpha 2 chains, although the alpha 1[I] and alpha 1[III] chains move at the same rate in the absence of urea. An attempt to separate the alpha 1[II] chain of type II collagen from the alpha 1[I] chain was unsuccessful under the experimental conditions employed.", "contents": "Separation of the alpha chains of type I and III collagens by SDS-polyacrylamide gel electrophoresis. A method for the separation of type III collagen from type I collagen by SDS-polyacrylamide gel electrophoresis has been developed. This is based on the observation that the presence of 3-4 M urea decreases the mobility of the alpha 1 [III] chain to a greater extent than those of the alpha 1[I] and alpha 2 chains, although the alpha 1[I] and alpha 1[III] chains move at the same rate in the absence of urea. An attempt to separate the alpha 1[II] chain of type II collagen from the alpha 1[I] chain was unsuccessful under the experimental conditions employed."}
{"id": "PMID:479140", "title": "Molecular arrangement of troponin-T in the thin filament.", "content": "1. Chymotrypsin cleaved troponin-T of skeletal muscle into two subfragments, i.e., troponin-T1 and -T2, each of which could be isolated by the use of DEAE-Sephadex. Troponin-T1 was a single subfragment with a molecular weight of 26,000 (chicken) or 22,000 (rabbit) daltons. Troponin-T2 consisted of two subfragments with molecular weights of about 13,000 daltons. Results obtained indicated that the smaller subfragment was formed by digestion of the larger subfragment of troponin-T2. 2. Antibodies against troponin-T1 and -T2 formed regular transverse striations along the whole length of thin filaments with 38 nm intervals, as was found reviously using antibodies against whole troponin complex as well as troponin components (Ohtsuki, I. et al., 1967; Ohtsuki, I. 1974 and 1975). 3. The first anti-troponin-T1 striation was situated 40 nm from the top of the filament. The first anti-troponin-T2 striation was 27 nm from the filament top and coincided with the first striations formed by antibodies against troponin-C or -I. 4. Troponin-T1 and the larger subfragment of troponin-T2 bound to tropomyosin which had been coupled to Sepharose, whereas the smaller subfragment of troponin-T2 did not.", "contents": "Molecular arrangement of troponin-T in the thin filament. 1. Chymotrypsin cleaved troponin-T of skeletal muscle into two subfragments, i.e., troponin-T1 and -T2, each of which could be isolated by the use of DEAE-Sephadex. Troponin-T1 was a single subfragment with a molecular weight of 26,000 (chicken) or 22,000 (rabbit) daltons. Troponin-T2 consisted of two subfragments with molecular weights of about 13,000 daltons. Results obtained indicated that the smaller subfragment was formed by digestion of the larger subfragment of troponin-T2. 2. Antibodies against troponin-T1 and -T2 formed regular transverse striations along the whole length of thin filaments with 38 nm intervals, as was found reviously using antibodies against whole troponin complex as well as troponin components (Ohtsuki, I. et al., 1967; Ohtsuki, I. 1974 and 1975). 3. The first anti-troponin-T1 striation was situated 40 nm from the top of the filament. The first anti-troponin-T2 striation was 27 nm from the filament top and coincided with the first striations formed by antibodies against troponin-C or -I. 4. Troponin-T1 and the larger subfragment of troponin-T2 bound to tropomyosin which had been coupled to Sepharose, whereas the smaller subfragment of troponin-T2 did not."}
{"id": "PMID:479141", "title": "Factors affecting the transient tension change after applying stepwise length change to glycerol-treated muscle fibers. Effects of temperature, divalent cations, and modification with p-chloromercuribenzoate.", "content": "The dynamic properties of cross-bridge movement were investigated in glycerol-treated muscle fibers under various conditions by analyzing tension responses to two types of length change. First, the fiber bundles were stretched linearly with time for 0.3 s from the rest length (L0) by 2.5% of L0, suddenly released, then fixed at L0 (sudden release of the slow stretch). Second, they were stretched for 0.01 s by 2.5% of L0, then held at the plateau length (a quick stretch). 1. The transient tension responses following both length changes were divided into three phases: (i) very quick recovery of tension (0 approximately 0.05 s), (ii) quick recovery (0.05 approximately 0.3-0.4 s), and (iii) gradual recovery (0.3-0.4 s approximately several seconds). 2. The effects of activating conditions on the rates of the quick phases (0 approximately 0.3-0.4 s) were not associated with those on the nucleoside triphosphatase [EC 3.6.1.3] rates: the rates of the quick phases increased with increase in temperature and Mg2+-ATP concentration, with decrease in Ca2+ concentration, and also on replacement of Mg2+-ATP by Mg2+-ITP or Mn2+-ATP. Only a small amount of ADP, 0.07 mol per mol of myosin (Fig. 24 in the preceding paper), was liberated during the quick recovery phases. 3. The remaining slow tension recovery was concluded to be associated with one cycle of ATP splitting, and progressed very smoothly. This suggests that most of the cross-bridges do not exist in a synchronously dissociated state during one cycle of ATP splitting.", "contents": "Factors affecting the transient tension change after applying stepwise length change to glycerol-treated muscle fibers. Effects of temperature, divalent cations, and modification with p-chloromercuribenzoate. The dynamic properties of cross-bridge movement were investigated in glycerol-treated muscle fibers under various conditions by analyzing tension responses to two types of length change. First, the fiber bundles were stretched linearly with time for 0.3 s from the rest length (L0) by 2.5% of L0, suddenly released, then fixed at L0 (sudden release of the slow stretch). Second, they were stretched for 0.01 s by 2.5% of L0, then held at the plateau length (a quick stretch). 1. The transient tension responses following both length changes were divided into three phases: (i) very quick recovery of tension (0 approximately 0.05 s), (ii) quick recovery (0.05 approximately 0.3-0.4 s), and (iii) gradual recovery (0.3-0.4 s approximately several seconds). 2. The effects of activating conditions on the rates of the quick phases (0 approximately 0.3-0.4 s) were not associated with those on the nucleoside triphosphatase [EC 3.6.1.3] rates: the rates of the quick phases increased with increase in temperature and Mg2+-ATP concentration, with decrease in Ca2+ concentration, and also on replacement of Mg2+-ATP by Mg2+-ITP or Mn2+-ATP. Only a small amount of ADP, 0.07 mol per mol of myosin (Fig. 24 in the preceding paper), was liberated during the quick recovery phases. 3. The remaining slow tension recovery was concluded to be associated with one cycle of ATP splitting, and progressed very smoothly. This suggests that most of the cross-bridges do not exist in a synchronously dissociated state during one cycle of ATP splitting."}
{"id": "PMID:479142", "title": "Degradation of rat cardiac myofibrils and myofibrillar proteins by a myosin-cleaving protease.", "content": "The degradation of rat cardiac myofibrils and their constituent proteins with a myosin-cleaving protease was studied. Electrophoretograms of the digestion products of myofibrils showed that myosin,M-protein, C-protein, and troponin were degraded, but actin and tropomyosin were not. Degradation of these constituents resulted in losses of the Mg2+-ATPase activity and its Ca2+-sensitivity of myofibrils. Incubation of myofibrils with the protease induced the release of alpha-actinin without degradation. Susceptibilities of myosin, actin, troponin, and alpha-actinin purified from rat and pig hearts to the protease were essentially identical to those of the assembled forms in myofibrils. Although the purified tropomyosin was readily degraded into five fragments with the protease, the tropomyosin assembled in myofibrils and actin-tropomyosin complex were insusceptible to the protease. Digestion of myosin in the filamentous state with the protease resulted in the disappearance of myosin heavy chain and light chain 2, producing two fragments having molecular weights of 130,000 and 94,000 which originated from the degradation of heavy chain. The Ca2+- and EDTA-ATPase activities of the degradation products remained unchanged during incubation for 22 h. The actin-activated ATPase activity of myosin was reduced by 30% during incubation for 6 h, and recovered to the original level on adding actin to give a ratio of actin to myosin of 2:1. The pH optima for degradation of myosin in the soluble and filamentous states were 8.5 and 7.0, respectively. The results indicate that cardiac myosin in the filamentous state was more readily degraded with the protease than the myosin in the soluble state.", "contents": "Degradation of rat cardiac myofibrils and myofibrillar proteins by a myosin-cleaving protease. The degradation of rat cardiac myofibrils and their constituent proteins with a myosin-cleaving protease was studied. Electrophoretograms of the digestion products of myofibrils showed that myosin,M-protein, C-protein, and troponin were degraded, but actin and tropomyosin were not. Degradation of these constituents resulted in losses of the Mg2+-ATPase activity and its Ca2+-sensitivity of myofibrils. Incubation of myofibrils with the protease induced the release of alpha-actinin without degradation. Susceptibilities of myosin, actin, troponin, and alpha-actinin purified from rat and pig hearts to the protease were essentially identical to those of the assembled forms in myofibrils. Although the purified tropomyosin was readily degraded into five fragments with the protease, the tropomyosin assembled in myofibrils and actin-tropomyosin complex were insusceptible to the protease. Digestion of myosin in the filamentous state with the protease resulted in the disappearance of myosin heavy chain and light chain 2, producing two fragments having molecular weights of 130,000 and 94,000 which originated from the degradation of heavy chain. The Ca2+- and EDTA-ATPase activities of the degradation products remained unchanged during incubation for 22 h. The actin-activated ATPase activity of myosin was reduced by 30% during incubation for 6 h, and recovered to the original level on adding actin to give a ratio of actin to myosin of 2:1. The pH optima for degradation of myosin in the soluble and filamentous states were 8.5 and 7.0, respectively. The results indicate that cardiac myosin in the filamentous state was more readily degraded with the protease than the myosin in the soluble state."}
{"id": "PMID:479143", "title": "Interactions of BPN' and Carlsberg subtilisins with peptides containing aromatic amino acids at the C-terminus. Specific rate enhancement due to the secondary enzyme-substrate interaction.", "content": "The interaction between BPN' or Carlsberg subtilisins and peptides of the type Ac-Glyn-X-OMe (n = 0, 1, 2, 3), where X denotes one of five different aromatic amino acids, was investigated to elucidate the effect of the secondary interaction on catalysis in relation to the nature of the X residue. The increase in interaction upon elongation of the chain was accompanied by a large increase in kcat but with no marked change in Km in all the series of sensitive substrates. The peptides containing 2-(2-nitro-4-carboxyphenylsulfenyl)-tryptophan, however, acted as competitive inhibitors and exhibited an invariant dissociation constant in spite of the different chain lengths. These observations suggest that the secondary enzyme-substrate interaction induces a conformational change in the active site of the enzyme or in the substrate in such a way as to lower the activation energy and to form a stabilized transient complex. In this respect, BPN' and Carlsberg subtilisins are similar to porcine pepsin and Streptomyces griseus protease 1 rather than to alpha-chymotrypsin.", "contents": "Interactions of BPN' and Carlsberg subtilisins with peptides containing aromatic amino acids at the C-terminus. Specific rate enhancement due to the secondary enzyme-substrate interaction. The interaction between BPN' or Carlsberg subtilisins and peptides of the type Ac-Glyn-X-OMe (n = 0, 1, 2, 3), where X denotes one of five different aromatic amino acids, was investigated to elucidate the effect of the secondary interaction on catalysis in relation to the nature of the X residue. The increase in interaction upon elongation of the chain was accompanied by a large increase in kcat but with no marked change in Km in all the series of sensitive substrates. The peptides containing 2-(2-nitro-4-carboxyphenylsulfenyl)-tryptophan, however, acted as competitive inhibitors and exhibited an invariant dissociation constant in spite of the different chain lengths. These observations suggest that the secondary enzyme-substrate interaction induces a conformational change in the active site of the enzyme or in the substrate in such a way as to lower the activation energy and to form a stabilized transient complex. In this respect, BPN' and Carlsberg subtilisins are similar to porcine pepsin and Streptomyces griseus protease 1 rather than to alpha-chymotrypsin."}
{"id": "PMID:479144", "title": "Studies of a calcium-activated neutral protease from chicken skeletal muscle. II. Substrate specificity.", "content": "Calcium-activated neutral protease purified from chicken skeletal muscle hydrolyzed myofibrillar proteins, tubulin, spectrin, and oxidized insulin B chain, but hardly hydrolyzed synthetic or natural peptides.", "contents": "Studies of a calcium-activated neutral protease from chicken skeletal muscle. II. Substrate specificity. Calcium-activated neutral protease purified from chicken skeletal muscle hydrolyzed myofibrillar proteins, tubulin, spectrin, and oxidized insulin B chain, but hardly hydrolyzed synthetic or natural peptides."}
{"id": "PMID:479145", "title": "The association between major sialoglycoprotein and spectrin of human erythrocyte membranes as detected by fluorescence resonance energy transfer.", "content": "The interactions among the major sialoglycoprotein and peripheral proteins of human erythrocyte membranes were investigated by the long range resonance energy transfer between different fluorescent moieties separately conjugated to proteins. Consequently, direct association between the major sialoglycoprotein and spectrin was observed and divalent cations were required for their association.", "contents": "The association between major sialoglycoprotein and spectrin of human erythrocyte membranes as detected by fluorescence resonance energy transfer. The interactions among the major sialoglycoprotein and peripheral proteins of human erythrocyte membranes were investigated by the long range resonance energy transfer between different fluorescent moieties separately conjugated to proteins. Consequently, direct association between the major sialoglycoprotein and spectrin was observed and divalent cations were required for their association."}
{"id": "PMID:479146", "title": "Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A.", "content": "The 270 MHZ proton MNR spectra of an aqueous solution of ribonuclease A in the presence of hexacyanochromate ions were measured. The C2 and C4 proton signals of the His 12 and His 119 residues were selectively broadened, indicating that this ion was bound to the active site. From analyses of concentration dependences of diamagnetic shifts, one hexacyanocobaltate ion was found to bind to the active site, with the dissociation constant of 8 mM. The paramagnetic hexacyanochromate ion is useful for mapping the active sites of enzymes for anionic substrates.", "contents": "Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. The 270 MHZ proton MNR spectra of an aqueous solution of ribonuclease A in the presence of hexacyanochromate ions were measured. The C2 and C4 proton signals of the His 12 and His 119 residues were selectively broadened, indicating that this ion was bound to the active site. From analyses of concentration dependences of diamagnetic shifts, one hexacyanocobaltate ion was found to bind to the active site, with the dissociation constant of 8 mM. The paramagnetic hexacyanochromate ion is useful for mapping the active sites of enzymes for anionic substrates."}
{"id": "PMID:479147", "title": "Purification of immunologically active HLA-A and -B antigens by a series of monoclonal antibody columns.", "content": "Serologically and biochemically pure preparations of detergent and papain-solubilized HLA-A2 and HLA-B7 antigens were isolated by high pH elution from a series of immunoaffinity columns constructed from monoclonal antibodies with specificity for HLA-A2 and HLA-B7 antigens. These preparations retained the immunological activity and quaternary structure of the native molecule and should provide suitable reagents for insertion into liposomes and probing the role of major histocompatibility antigens in lymphocyte interactions.", "contents": "Purification of immunologically active HLA-A and -B antigens by a series of monoclonal antibody columns. Serologically and biochemically pure preparations of detergent and papain-solubilized HLA-A2 and HLA-B7 antigens were isolated by high pH elution from a series of immunoaffinity columns constructed from monoclonal antibodies with specificity for HLA-A2 and HLA-B7 antigens. These preparations retained the immunological activity and quaternary structure of the native molecule and should provide suitable reagents for insertion into liposomes and probing the role of major histocompatibility antigens in lymphocyte interactions."}
{"id": "PMID:479148", "title": "Purification of the H-2Kk molecule of the murine major histocompatibility complex.", "content": "The intact H-2Kk antigen has been detergent-solubilized and purified using an immunoabsorbent column prepared from the 11-4.1 monoclonal antibody described by Oi et al. (Oi, V. T., Jones, P. P., Goding, J. Current Topics in Microbiology and Immunology (Melchers, F., Potter, M., and Warner, N. L., eds) Vol. 81, pp. 115-129, Springer-Verlag, New York). The mild conditions used for elution from the column, 0.5% deoxycholate in 10 mM Tris buffer, pH 8, with 0.14 M NaCl, result in recovery of 70 to 100% of the allogeneic serological activity. A murine lymphoma, RDM-4, was found to express high levels of H2-Kk; approximately 2 X 10(6) molecules/cell. Milligram quantities of H-2Kk can be purified readily using these cells.", "contents": "Purification of the H-2Kk molecule of the murine major histocompatibility complex. The intact H-2Kk antigen has been detergent-solubilized and purified using an immunoabsorbent column prepared from the 11-4.1 monoclonal antibody described by Oi et al. (Oi, V. T., Jones, P. P., Goding, J. Current Topics in Microbiology and Immunology (Melchers, F., Potter, M., and Warner, N. L., eds) Vol. 81, pp. 115-129, Springer-Verlag, New York). The mild conditions used for elution from the column, 0.5% deoxycholate in 10 mM Tris buffer, pH 8, with 0.14 M NaCl, result in recovery of 70 to 100% of the allogeneic serological activity. A murine lymphoma, RDM-4, was found to express high levels of H2-Kk; approximately 2 X 10(6) molecules/cell. Milligram quantities of H-2Kk can be purified readily using these cells."}
{"id": "PMID:479149", "title": "A new polyamine, thermospermine, 1,12-diamino-4,8-diazadodecane, from an extreme thermophile.", "content": "A new polyamine has been extracted from an extreme thermophile, Thermus thermophilus, and its chemical structure was determined as 1,12-diamino-4,8-diazadodecane, NH2(CH2)3NH(CH2)3NH(CH2)4NH2, based on its proton NMR, 13C NMR, and mass spectra. A trivial name \"thermospermine\" is proposed for the new compound.", "contents": "A new polyamine, thermospermine, 1,12-diamino-4,8-diazadodecane, from an extreme thermophile. A new polyamine has been extracted from an extreme thermophile, Thermus thermophilus, and its chemical structure was determined as 1,12-diamino-4,8-diazadodecane, NH2(CH2)3NH(CH2)3NH(CH2)4NH2, based on its proton NMR, 13C NMR, and mass spectra. A trivial name \"thermospermine\" is proposed for the new compound."}
{"id": "PMID:479150", "title": "Dissociation of thrombin from platelets by hirudin. Evidence for receptor processing.", "content": "Hirudin inhibited the binding of human 125I-alpha-thrombin to the saturable, but not the nonsaturable, sites on washed human platelets. When hirudin was added to a thrombin-platelet mixture, it caused a biphasic dissociation of bound thrombin. A partial dissociation was too rapid to measure and was followed by complete dissociation with a first order rate constant of about 10(-2) s-1. The fraction of bound thrombin in the more slowly dissociable form increased from essentially none after a 5-s preincubation of thrombin and platelets to as much as 75% of saturable binding after a 4-min preincubation. Transition to the slowly dissociable state was not accompanied by an increase in the amount bound and was not observed with active site serine-derivatized thrombin. This is the first evidence with intact platelets of a binding characteristic that depends, as does platelet stimulation, on catalytically active thrombin, suggesting that it may represent physiologically significant receptor processing.", "contents": "Dissociation of thrombin from platelets by hirudin. Evidence for receptor processing. Hirudin inhibited the binding of human 125I-alpha-thrombin to the saturable, but not the nonsaturable, sites on washed human platelets. When hirudin was added to a thrombin-platelet mixture, it caused a biphasic dissociation of bound thrombin. A partial dissociation was too rapid to measure and was followed by complete dissociation with a first order rate constant of about 10(-2) s-1. The fraction of bound thrombin in the more slowly dissociable form increased from essentially none after a 5-s preincubation of thrombin and platelets to as much as 75% of saturable binding after a 4-min preincubation. Transition to the slowly dissociable state was not accompanied by an increase in the amount bound and was not observed with active site serine-derivatized thrombin. This is the first evidence with intact platelets of a binding characteristic that depends, as does platelet stimulation, on catalytically active thrombin, suggesting that it may represent physiologically significant receptor processing."}
{"id": "PMID:479152", "title": "Glucagon stimulation of liver mitochondrial CO2 fixation utilizing pyruvate generated inside the mitochondria.", "content": "Glucagon administration to the intact rat has been shown to stimulate pyruvate metabolism in liver mitochondria, presumably by increasing pyruvate transport into the organelle. In this report, we used alanine in place of pyruvate to examine the possibility that glucagon might stimulate pyruvate carboxylation per se independent of its postulated action on pyruvate transport. In agreement with previous reports, injection of a low dose of glucagon (50 micrograms/kg of rat) increased respiration, ATP synthesis, pyruvate decarboxylation, and CO2 fixation in liver mitochondria subsequently isolated. When alanine was used as a substrate, CO2 fixation, but not decarboxylation, was increased in liver mitochondria isolated from glucagon-treated rats. Pyruvate accumulation under these conditions was significantly lower in the glucagon-treated rat preparation. When mitochondria were incubated in a HCO3- -deficient buffer, pyruvate accumulation was identical in both preparations. The addition of a pyruvate transport inhibitor, alpha-cyanohydroxycinnamate (0.5 mM), inhibited CO2 fixation with pyruvate by 70%, but had no effect when alanine was used. Our data therefore suggest that glucagon stimluates mitochondrial pyruvate carboxylation independent of its possible action on pyruvate transport.", "contents": "Glucagon stimulation of liver mitochondrial CO2 fixation utilizing pyruvate generated inside the mitochondria. Glucagon administration to the intact rat has been shown to stimulate pyruvate metabolism in liver mitochondria, presumably by increasing pyruvate transport into the organelle. In this report, we used alanine in place of pyruvate to examine the possibility that glucagon might stimulate pyruvate carboxylation per se independent of its postulated action on pyruvate transport. In agreement with previous reports, injection of a low dose of glucagon (50 micrograms/kg of rat) increased respiration, ATP synthesis, pyruvate decarboxylation, and CO2 fixation in liver mitochondria subsequently isolated. When alanine was used as a substrate, CO2 fixation, but not decarboxylation, was increased in liver mitochondria isolated from glucagon-treated rats. Pyruvate accumulation under these conditions was significantly lower in the glucagon-treated rat preparation. When mitochondria were incubated in a HCO3- -deficient buffer, pyruvate accumulation was identical in both preparations. The addition of a pyruvate transport inhibitor, alpha-cyanohydroxycinnamate (0.5 mM), inhibited CO2 fixation with pyruvate by 70%, but had no effect when alanine was used. Our data therefore suggest that glucagon stimluates mitochondrial pyruvate carboxylation independent of its possible action on pyruvate transport."}
{"id": "PMID:479154", "title": "Hybrid formation for liganded hemoglobins A and C at subzero temperatures.", "content": "The kinetics of formation of the asymmetric carbonmonoxyhemoglobin hybrid (alpha beta)A(alpha beta)C from the parent molecules alpha 2 beta 2A and alpha 2 beta 2C have been studied by electrophoresis at subzero temperatures (down to -40 degrees C) using as supporting media gels of acrylamide/methylacrylate in dimethyl sulfoxide/water mixtures. It has been found that in these media the rate of hybrid formation is markedly affected by pH and decreases by an order of magnitude between pH 7.3 and 8.3. At pH greater than 10, t = -40 degrees C, the hybrid between alpha 2 beta 2A and alpha 2 beta 2C is stable for several hours. A rapid thermal quenching of a mixture of alpha 2 beta 2A and alpha 2 beta 2C prevented hybrid formation during the time required to separate the 2 molecules.", "contents": "Hybrid formation for liganded hemoglobins A and C at subzero temperatures. The kinetics of formation of the asymmetric carbonmonoxyhemoglobin hybrid (alpha beta)A(alpha beta)C from the parent molecules alpha 2 beta 2A and alpha 2 beta 2C have been studied by electrophoresis at subzero temperatures (down to -40 degrees C) using as supporting media gels of acrylamide/methylacrylate in dimethyl sulfoxide/water mixtures. It has been found that in these media the rate of hybrid formation is markedly affected by pH and decreases by an order of magnitude between pH 7.3 and 8.3. At pH greater than 10, t = -40 degrees C, the hybrid between alpha 2 beta 2A and alpha 2 beta 2C is stable for several hours. A rapid thermal quenching of a mixture of alpha 2 beta 2A and alpha 2 beta 2C prevented hybrid formation during the time required to separate the 2 molecules."}
{"id": "PMID:479158", "title": "Carbohydrate of the human plasminogen variants. III. Structure of the O-glycosidically linked oligosaccharide unit.", "content": "The preceding two manuscripts in this issue (Hayes, M. L., and Castellino, F. J. (1979) J. Biol. Chem. 254, 8768-8771, 8772-8776) describe the isolation and characterization of glycopeptides from human plasminogen affinity chromatography variants 1 and 2. Plasminogen variant 1 contains an asparagine288-based branched carbohydrate structure, which has been established in the immediately preceding manuscript. This structure is absent in variant 2. Plasminogen variants 1 and 2 contain a threonine-based glycoconjugate. This latter structure has been established by combination of methylation data, glycosidase digestions, periodate oxidations, and Smith degradations of the beta-eliminated reduced oligosaccharides. One glycopeptide unit, isolated from both plasminogen variants 1 (1D) and 2 (2D) possessed the following structure: Sia alpha 2 yields 3Gal beta 1 yields 3GalNAc-Thr. The threonine was found to be residue 345 in the Glu-plasminogen sequence. Another glycopeptide unit was also found to be present, in lower yields in both variants 1 (1E) and 2 (2E). The structure of this unit was: Sia alpha 2 yields 3Gal beta1 yields 3GalNAc-Thr. : formula: (see text), alpha 2,6 Sia. Again Thr 345 was the glycosylated amino acid. The amino acid sequence around the glycosylated threonine was found to be NH2-Ala.Pro.Thr(CHO).Ala.Pro.Pro.Glu.", "contents": "Carbohydrate of the human plasminogen variants. III. Structure of the O-glycosidically linked oligosaccharide unit. The preceding two manuscripts in this issue (Hayes, M. L., and Castellino, F. J. (1979) J. Biol. Chem. 254, 8768-8771, 8772-8776) describe the isolation and characterization of glycopeptides from human plasminogen affinity chromatography variants 1 and 2. Plasminogen variant 1 contains an asparagine288-based branched carbohydrate structure, which has been established in the immediately preceding manuscript. This structure is absent in variant 2. Plasminogen variants 1 and 2 contain a threonine-based glycoconjugate. This latter structure has been established by combination of methylation data, glycosidase digestions, periodate oxidations, and Smith degradations of the beta-eliminated reduced oligosaccharides. One glycopeptide unit, isolated from both plasminogen variants 1 (1D) and 2 (2D) possessed the following structure: Sia alpha 2 yields 3Gal beta 1 yields 3GalNAc-Thr. The threonine was found to be residue 345 in the Glu-plasminogen sequence. Another glycopeptide unit was also found to be present, in lower yields in both variants 1 (1E) and 2 (2E). The structure of this unit was: Sia alpha 2 yields 3Gal beta1 yields 3GalNAc-Thr. : formula: (see text), alpha 2,6 Sia. Again Thr 345 was the glycosylated amino acid. The amino acid sequence around the glycosylated threonine was found to be NH2-Ala.Pro.Thr(CHO).Ala.Pro.Pro.Glu."}
{"id": "PMID:479159", "title": "The sites of the lactoperoxidase-catalyzed iodination of human fibrinogen.", "content": "A study of those tyrosines in fibrinogen which are surface-oriented and which may be involved in polymerization has been investigated using as a probe iodination catalyzed by lactoperoxidase. The iodine distribution in the major cyanogen bromide fragments was studied. A fragment of the B beta chain extending beyond residue 118 had the highest specific activity. Tyrosine 119 was identified as the residue most susceptible to iodination. There was no difference in susceptibility to iodination of N-DSK (A alpha 1-51, B beta 1-118, gamma 1-78)2, Ho1-DSK (first hydrophobic disulfide knot), and Hi2-DSK (second hydrophobic disulfide knot). Tyrosines 18 and 32 of the gamma chain of N-DSK were not significantly iodinated in fibrinogen, but tyrosines 1 and 68 were labeled, as was the tyrosine of the A alpha chain. The data indicate that there are regions of the hydrophobic disulfide knot, Ho1-DSK, which are surface-oriented. The distribution of iodine as mono- and diiodotyrosine in N-DSK and Ho1-DSK reflected the percentage (83 and 17, respectively) found in iodinated fibrinogen from which these fragments were prepared. In contrast the segments of the B beta chain extending beyond Met118 contained 46% of the iodine in diiodotyrosine, while the A alpha chain fragment, Hi2-DSK, contained 28% as diiodotyrosine. No significant iodination of histidine was detected.", "contents": "The sites of the lactoperoxidase-catalyzed iodination of human fibrinogen. A study of those tyrosines in fibrinogen which are surface-oriented and which may be involved in polymerization has been investigated using as a probe iodination catalyzed by lactoperoxidase. The iodine distribution in the major cyanogen bromide fragments was studied. A fragment of the B beta chain extending beyond residue 118 had the highest specific activity. Tyrosine 119 was identified as the residue most susceptible to iodination. There was no difference in susceptibility to iodination of N-DSK (A alpha 1-51, B beta 1-118, gamma 1-78)2, Ho1-DSK (first hydrophobic disulfide knot), and Hi2-DSK (second hydrophobic disulfide knot). Tyrosines 18 and 32 of the gamma chain of N-DSK were not significantly iodinated in fibrinogen, but tyrosines 1 and 68 were labeled, as was the tyrosine of the A alpha chain. The data indicate that there are regions of the hydrophobic disulfide knot, Ho1-DSK, which are surface-oriented. The distribution of iodine as mono- and diiodotyrosine in N-DSK and Ho1-DSK reflected the percentage (83 and 17, respectively) found in iodinated fibrinogen from which these fragments were prepared. In contrast the segments of the B beta chain extending beyond Met118 contained 46% of the iodine in diiodotyrosine, while the A alpha chain fragment, Hi2-DSK, contained 28% as diiodotyrosine. No significant iodination of histidine was detected."}
{"id": "PMID:479160", "title": "Purification and characterization of a ribosome dissociation factor (eukaryotic initiation factor 6) from wheat germ.", "content": "A wheat germ ribosome dissociation factor, eukaryotic initiation factor 6 (eIF-6), has been purified almost to homogeneity from the 25 to 40% ammonium sulfate fraction of the postribosomal supernatant. This dissociation factor is distinct from initiation factor eIF-3 and its chromatographic properties permit its separation from the known wheat germ initiation factors. Under certain conditions, eIF-6 stimulates the incorporation of amino acids into polypeptides in a partially fractionated wheat germ cell-free system. The eight-step purification procedure developed includes chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-75, and hydroxyapatite and yields a dissociation factor more than 80% pure. The purified factor is composed of a single polypeptide chain with a molecular weight of approximately 23,000 as determined by gel filtration chromatography and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It is an acidic protein which is heat labile and is inactivated by treatment with N-ethylmaleimide. The dissociation factor is much more effective in preventing the reassociation of 40 S and 60 S ribosomal subunits than in directly dissociating 80 S ribosomes. Like Escherichia coli IF-3, about 10 pmol of the dissociation factor are required to dissociate 1 pmol of ribosomes.", "contents": "Purification and characterization of a ribosome dissociation factor (eukaryotic initiation factor 6) from wheat germ. A wheat germ ribosome dissociation factor, eukaryotic initiation factor 6 (eIF-6), has been purified almost to homogeneity from the 25 to 40% ammonium sulfate fraction of the postribosomal supernatant. This dissociation factor is distinct from initiation factor eIF-3 and its chromatographic properties permit its separation from the known wheat germ initiation factors. Under certain conditions, eIF-6 stimulates the incorporation of amino acids into polypeptides in a partially fractionated wheat germ cell-free system. The eight-step purification procedure developed includes chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-75, and hydroxyapatite and yields a dissociation factor more than 80% pure. The purified factor is composed of a single polypeptide chain with a molecular weight of approximately 23,000 as determined by gel filtration chromatography and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It is an acidic protein which is heat labile and is inactivated by treatment with N-ethylmaleimide. The dissociation factor is much more effective in preventing the reassociation of 40 S and 60 S ribosomal subunits than in directly dissociating 80 S ribosomes. Like Escherichia coli IF-3, about 10 pmol of the dissociation factor are required to dissociate 1 pmol of ribosomes."}
{"id": "PMID:479161", "title": "Glycoprotein biosynthesis. Rat liver microsomal glucosidases which process oligosaccharides.", "content": "Asparagine-linked oligosaccharides of glycoproteins undergo extensive modification or \"processing\" following their attachment to protein. A key step in post-glycosylation processing is the sequential removal of glucose residues from the protein-linked oligosaccharide. We have studied rat liver preparations which catalyze removal of glucose from Glc3Man9GlcNAc, Glc2Man9GlcNAc, and Glc1Man9GlcNAc. Detergent solubilization studies, inhibitor studies, and temperature-activity profiles indicate that at least two distinct glucosidases are present in the membranes. One of these glucosidases removes the distal glucose from Glc3Man9GlcNAc, and the other glucosidase sequentially removes glucose from Glc2Man9GlcNAc and Glc1Man9GlcNAc. The latter glucosidase has been solubilized from the microsomal memrbranes and purified 12-fold. The glucosidases, which are integral membrane proteins, are localized in the rough and smooth microsomes and appear to be located on the cisternal surface of the microsomal vesicles. These glucosidases are suggested to be of biological importance in catalyzing the initial events in the post-glycosylation processing of cellular glycoprotein.", "contents": "Glycoprotein biosynthesis. Rat liver microsomal glucosidases which process oligosaccharides. Asparagine-linked oligosaccharides of glycoproteins undergo extensive modification or \"processing\" following their attachment to protein. A key step in post-glycosylation processing is the sequential removal of glucose residues from the protein-linked oligosaccharide. We have studied rat liver preparations which catalyze removal of glucose from Glc3Man9GlcNAc, Glc2Man9GlcNAc, and Glc1Man9GlcNAc. Detergent solubilization studies, inhibitor studies, and temperature-activity profiles indicate that at least two distinct glucosidases are present in the membranes. One of these glucosidases removes the distal glucose from Glc3Man9GlcNAc, and the other glucosidase sequentially removes glucose from Glc2Man9GlcNAc and Glc1Man9GlcNAc. The latter glucosidase has been solubilized from the microsomal memrbranes and purified 12-fold. The glucosidases, which are integral membrane proteins, are localized in the rough and smooth microsomes and appear to be located on the cisternal surface of the microsomal vesicles. These glucosidases are suggested to be of biological importance in catalyzing the initial events in the post-glycosylation processing of cellular glycoprotein."}
{"id": "PMID:479163", "title": "Syncatalytic modification of chicken liver xanthine dehydrogenase by hydrogen peroxide. The nature of the reaction.", "content": "Xanthine dehydrogenase engaged in catalyzing the oxidation of substrate by oxygen is repidly inactivated by the hydrogen peroxide generated during the reaction. Experimental evidence shows that peroxide reacts more readily with the reduced than with the oxidized form of the enzyme. Inactivation results from modification of the cyanolysable sulfur present at the molybdenum center.", "contents": "Syncatalytic modification of chicken liver xanthine dehydrogenase by hydrogen peroxide. The nature of the reaction. Xanthine dehydrogenase engaged in catalyzing the oxidation of substrate by oxygen is repidly inactivated by the hydrogen peroxide generated during the reaction. Experimental evidence shows that peroxide reacts more readily with the reduced than with the oxidized form of the enzyme. Inactivation results from modification of the cyanolysable sulfur present at the molybdenum center."}
{"id": "PMID:479165", "title": "Interfacial structure and lipase action. Characterization of taurodeoxycholate-didecanoylglycerol monolayers by physical and kinetic methods.", "content": "Surface pressure-area isotherms for 1,3-didecanoyl-glycerol (dicaprin) were determined as a function of the concentration of taurodeoxycholate in the subphase. Analysis of these curves indicates that, from 0.05 to 0.80 mM bile salt, surface structure is dependent only on the surface concentration of the diglyceride. The limiting areas for dicaprin in the presence and absence of bile salt were about 38 A2/molecule. Subjecting the monolayers to hydrolysis by pancreatic lipase yielded kinetic data which, together with the physical studies, support a model for monolayer glyceride molecules undergoing discrete changes of state. In the absence of bile salt, the relatively expanded state exhibits an area of 75 A2/diglyceride molecule and is not a substrate for pancreatic lipase B. The more condensed state exhibits an area of 38 A2/diglyceride molecule and is hydrolyzed at a rate proportional to its concentration in the monolayer. Taurodeoxybholate at 0.05 to 0.60 mM shifts the apparent area of the expanded state to 360 A2/diglyceride molecule.", "contents": "Interfacial structure and lipase action. Characterization of taurodeoxycholate-didecanoylglycerol monolayers by physical and kinetic methods. Surface pressure-area isotherms for 1,3-didecanoyl-glycerol (dicaprin) were determined as a function of the concentration of taurodeoxycholate in the subphase. Analysis of these curves indicates that, from 0.05 to 0.80 mM bile salt, surface structure is dependent only on the surface concentration of the diglyceride. The limiting areas for dicaprin in the presence and absence of bile salt were about 38 A2/molecule. Subjecting the monolayers to hydrolysis by pancreatic lipase yielded kinetic data which, together with the physical studies, support a model for monolayer glyceride molecules undergoing discrete changes of state. In the absence of bile salt, the relatively expanded state exhibits an area of 75 A2/diglyceride molecule and is not a substrate for pancreatic lipase B. The more condensed state exhibits an area of 38 A2/diglyceride molecule and is hydrolyzed at a rate proportional to its concentration in the monolayer. Taurodeoxybholate at 0.05 to 0.60 mM shifts the apparent area of the expanded state to 360 A2/diglyceride molecule."}
{"id": "PMID:479167", "title": "Accumulation of amino acids by lysosomes incubated with amino acid methyl esters.", "content": "Rat liver lysosomal preparations incubated with 10(-5) M L-[4,5-3H]leucine methyl ester hydrolyzed the methyl ester and accumulated radioactivity within a particulate compartment. The acculated radioactivity was identified as free leucine by thin layer chromatography. Free leucine was not itself taken up by the lysosomal preparations. The capacity to accumulate leucine was identified as a specific property of lysosomes and was thought to result from the trapping of the free amino acid within the lysosome following the hydrolysis of the methyl ester. Lysosomes also accumulated phenylalanine, serine, and alanine when incubated with the corresponding methyl esters. Leucine accumulation was inhibited by submillimolar concentrations of chloroquine, by the protease inhibitor L-1-tosylamido-2-phenylethyl chloromethyl ketone, and by lowering the pH below 7.0. Efflux of leucine from the lysosomes was highly temperature dependent (activation energy 33 kcal/mol). No evidence was found to suggest that leucine efflux was a carrier-mediated process. The results provide a new methodology for the study of amino acid movements across lysosomal membranes.", "contents": "Accumulation of amino acids by lysosomes incubated with amino acid methyl esters. Rat liver lysosomal preparations incubated with 10(-5) M L-[4,5-3H]leucine methyl ester hydrolyzed the methyl ester and accumulated radioactivity within a particulate compartment. The acculated radioactivity was identified as free leucine by thin layer chromatography. Free leucine was not itself taken up by the lysosomal preparations. The capacity to accumulate leucine was identified as a specific property of lysosomes and was thought to result from the trapping of the free amino acid within the lysosome following the hydrolysis of the methyl ester. Lysosomes also accumulated phenylalanine, serine, and alanine when incubated with the corresponding methyl esters. Leucine accumulation was inhibited by submillimolar concentrations of chloroquine, by the protease inhibitor L-1-tosylamido-2-phenylethyl chloromethyl ketone, and by lowering the pH below 7.0. Efflux of leucine from the lysosomes was highly temperature dependent (activation energy 33 kcal/mol). No evidence was found to suggest that leucine efflux was a carrier-mediated process. The results provide a new methodology for the study of amino acid movements across lysosomal membranes."}
{"id": "PMID:479171", "title": "Reversible uncoupling of photophosphorylation by a new bifunctional maleimide.", "content": "A new bifunctional maleimide that contains a disulfide bond has been synthesized. This maleimide, dithiobis-N-ethylmaleimide (DTEM), like another bifunctional maleimide, o-phenylenebismaleimide, is about 500-fold more effective as an inhibitor of photophosphorylation than N-ethylmaleimide. Thylakoids must be illuminated in the presence of DTEM before the assay of phosphorylation for the inhibition to occur. Phosphoryalation in thylakoids treated with DTEM in the light is uncoupled and proton permeability of the treated thylakoids is enhanced. This uncoupling of photophosphorylation in thylakoids treated with DTEM can be reversed by thiol compounds. The addition of 50 mM dithiothreitol restores H+ uptake in thylakoids treated with DTEM in the light to control levels and partially reverses the inhibition of phosphorylation. Evidence is provided to show that DTEM cross-links groups within the gamma subunit of the coupling factor 1, and that the cross-link is broken by high concentrations of thiols. These results suggest that cross-linking is the cause for the increased proton permeability in thylakoids treated with bifunctional maleimides in the light.", "contents": "Reversible uncoupling of photophosphorylation by a new bifunctional maleimide. A new bifunctional maleimide that contains a disulfide bond has been synthesized. This maleimide, dithiobis-N-ethylmaleimide (DTEM), like another bifunctional maleimide, o-phenylenebismaleimide, is about 500-fold more effective as an inhibitor of photophosphorylation than N-ethylmaleimide. Thylakoids must be illuminated in the presence of DTEM before the assay of phosphorylation for the inhibition to occur. Phosphoryalation in thylakoids treated with DTEM in the light is uncoupled and proton permeability of the treated thylakoids is enhanced. This uncoupling of photophosphorylation in thylakoids treated with DTEM can be reversed by thiol compounds. The addition of 50 mM dithiothreitol restores H+ uptake in thylakoids treated with DTEM in the light to control levels and partially reverses the inhibition of phosphorylation. Evidence is provided to show that DTEM cross-links groups within the gamma subunit of the coupling factor 1, and that the cross-link is broken by high concentrations of thiols. These results suggest that cross-linking is the cause for the increased proton permeability in thylakoids treated with bifunctional maleimides in the light."}
{"id": "PMID:479172", "title": "Adenine nucleotide degradation during energy depletion in human lymphoblasts. Adenosine accumulation and adenylate energy charge correlation.", "content": "Adenine nucleotide breakdown to nucleosides and purine bases was measured in cultures of human lymphoblastoid cells following: 1) the inhibition of oxidative phosphorylation in the absence of glucose or 2) the addition of 2-deoxyglucose. A mutant cell line, deficient in adenosine kinase, in the presence of an adenosine deaminase inhibitor was used to measure utilization of the two pathways of AMP catabolism involving initial action of either purine 5'-nucleotidase or AMP deaminase. In such a system the appearance of adenosine induced by the oxidative phosphorylation inhibitor, rotenone, implies that approximately 70% of AMP breakdown occurs via dephosphorylation. By the same method, deamination accounts for 82% of AMP breakdown when 2-deoxyglucose is added. The occurrence of AMP dephosphorylation is not correlated with elevated concentrations of substrate or with decreased concentrations of the inhibitors of 5'-nucleotidase, ATP and ADP. Dephosphorylation occurs if, and only if, the adenylate energy charge decreases to about 0.6 in these experiments. In cultures deprived of glucose and oxygen, adenine nucleotide degradation via dephosphorylation results in recovery of normal energy charge values.", "contents": "Adenine nucleotide degradation during energy depletion in human lymphoblasts. Adenosine accumulation and adenylate energy charge correlation. Adenine nucleotide breakdown to nucleosides and purine bases was measured in cultures of human lymphoblastoid cells following: 1) the inhibition of oxidative phosphorylation in the absence of glucose or 2) the addition of 2-deoxyglucose. A mutant cell line, deficient in adenosine kinase, in the presence of an adenosine deaminase inhibitor was used to measure utilization of the two pathways of AMP catabolism involving initial action of either purine 5'-nucleotidase or AMP deaminase. In such a system the appearance of adenosine induced by the oxidative phosphorylation inhibitor, rotenone, implies that approximately 70% of AMP breakdown occurs via dephosphorylation. By the same method, deamination accounts for 82% of AMP breakdown when 2-deoxyglucose is added. The occurrence of AMP dephosphorylation is not correlated with elevated concentrations of substrate or with decreased concentrations of the inhibitors of 5'-nucleotidase, ATP and ADP. Dephosphorylation occurs if, and only if, the adenylate energy charge decreases to about 0.6 in these experiments. In cultures deprived of glucose and oxygen, adenine nucleotide degradation via dephosphorylation results in recovery of normal energy charge values."}
{"id": "PMID:479176", "title": "Adenylate deaminase from rat muscle. Regulation by purine nucleotides and orthophosphate in the presence of 150 mM KCl.", "content": "Adenylate deaminase from rat skeletal muscle has been studied with the objective of understanding how the activity of the enzyme is regulated in vivo. ATP and GTP inhibit the enzyme at low concentrations in the presence of 150 mM KCl. The ATP inhibition is reversed as the ATP concentration is raised to physiological levels. The GTP inhibition is reversed as the GTP concentration is raised to unphysiologically high levels. In the presence of physiological concentrations of ATP, the GTP inhibition is also greatly diminished, but inhibition by orthophosphate remains strong. The apparent affinities of the enzyme for GTP, ATP, and orthophosphate are reduced as the pH is decreased from 7.0 to 6.2. ADP also reduces the apparent affinities of the enzyme for the inhibitors. The regulatory effects of GTP, ATP, and ADP are produced primarily by their unchelated forms. Comparison of the kinetic behavior of the enzyme in vitro with metabolite concentrations in vivo indicates that the major variables that regulate the activity of adenylate deaminase of muscle in vivo are the concentrations of AMP, ADP, orthophosphate, and H+.", "contents": "Adenylate deaminase from rat muscle. Regulation by purine nucleotides and orthophosphate in the presence of 150 mM KCl. Adenylate deaminase from rat skeletal muscle has been studied with the objective of understanding how the activity of the enzyme is regulated in vivo. ATP and GTP inhibit the enzyme at low concentrations in the presence of 150 mM KCl. The ATP inhibition is reversed as the ATP concentration is raised to physiological levels. The GTP inhibition is reversed as the GTP concentration is raised to unphysiologically high levels. In the presence of physiological concentrations of ATP, the GTP inhibition is also greatly diminished, but inhibition by orthophosphate remains strong. The apparent affinities of the enzyme for GTP, ATP, and orthophosphate are reduced as the pH is decreased from 7.0 to 6.2. ADP also reduces the apparent affinities of the enzyme for the inhibitors. The regulatory effects of GTP, ATP, and ADP are produced primarily by their unchelated forms. Comparison of the kinetic behavior of the enzyme in vitro with metabolite concentrations in vivo indicates that the major variables that regulate the activity of adenylate deaminase of muscle in vivo are the concentrations of AMP, ADP, orthophosphate, and H+."}
{"id": "PMID:479177", "title": "The insulin-like effect of hydrogen peroxide on pathways of lipid synthesis in rat adipocytes.", "content": "In addition to the well known insulin-like effects of certain concentrations of H2O2 on glucose transport and oxidation in isolated rat adipocytes, the present work demonstrates that lipid synthesis from glucose is also enhanced over a narrow range of H2O2 concentrations (0.15 to 0.5 mM) added to the incubation medium. As in the case of insulin, H2O2 was found to stimulate greater glucose incorporation into glyceride-fatty acid than incorporation into glyceride-glycerol. As part of a multifaceted regulation of lipogenesis, H2O2, like insulin, increased the amount of pyruvate dehydrogenase in the active form without increasing the total amount of pyruvate dehydrogenase. Pyruvate dehydrogenase activity increased within 5 min of H2O2 incubation, reached a maximum at 15 min and declined thereafter as the H2O2 disappeared from the incubation medium. While medium glucose per se was found to activate the enzyme, it is unlikely that the effect of H2O2 was mediated by the known enhancement of glucose transport since the effects on the enzyme were maximal in the absence of glucose in the incubation medium. These findings add to the growing list of insulin effects that are reproduced by H2O2, and strengthen the hypothesis that assigns H2O2 the role of \"second messenger\" of insulin.", "contents": "The insulin-like effect of hydrogen peroxide on pathways of lipid synthesis in rat adipocytes. In addition to the well known insulin-like effects of certain concentrations of H2O2 on glucose transport and oxidation in isolated rat adipocytes, the present work demonstrates that lipid synthesis from glucose is also enhanced over a narrow range of H2O2 concentrations (0.15 to 0.5 mM) added to the incubation medium. As in the case of insulin, H2O2 was found to stimulate greater glucose incorporation into glyceride-fatty acid than incorporation into glyceride-glycerol. As part of a multifaceted regulation of lipogenesis, H2O2, like insulin, increased the amount of pyruvate dehydrogenase in the active form without increasing the total amount of pyruvate dehydrogenase. Pyruvate dehydrogenase activity increased within 5 min of H2O2 incubation, reached a maximum at 15 min and declined thereafter as the H2O2 disappeared from the incubation medium. While medium glucose per se was found to activate the enzyme, it is unlikely that the effect of H2O2 was mediated by the known enhancement of glucose transport since the effects on the enzyme were maximal in the absence of glucose in the incubation medium. These findings add to the growing list of insulin effects that are reproduced by H2O2, and strengthen the hypothesis that assigns H2O2 the role of \"second messenger\" of insulin."}
{"id": "PMID:479181", "title": "Proton NMR study of methemoglobin and its isolated chains. Effect of the subunit association on the structure of the subunits.", "content": "In order to investigate the effect of the alpha beta subunit contacts on the subunit structure of human adult methemoglobin, the hyperfine shifted proton NMR spectra of several high spin complexes (water, cyanate, thiocyanate, formate, fluoride, and nitrite) and low spin complexes (imisazole, azide, and cyanide) of hemoglobin and its isolated subunits were characterized at 220 MHz and 22 degrees C. The spectra of ferric low spin derivatives of the isolated subunits were approximately superimposable on the corresponding hemoglobin spectra. On the other hand, the high spin spectra of the isolated subunits were greatly different from each other. The spectral anomaly in the ferric high spin complexes of the isolated beta subunit were interpreted to indicate other structural change than the hemichrome formation in the beta heme pocket. Difference in the subunit association effect between the high and low spin complexes of the isolated beta subunit was interpreted on the basis of a conformational change of the apoprotein dependent on the spin state of the beta heme iron.", "contents": "Proton NMR study of methemoglobin and its isolated chains. Effect of the subunit association on the structure of the subunits. In order to investigate the effect of the alpha beta subunit contacts on the subunit structure of human adult methemoglobin, the hyperfine shifted proton NMR spectra of several high spin complexes (water, cyanate, thiocyanate, formate, fluoride, and nitrite) and low spin complexes (imisazole, azide, and cyanide) of hemoglobin and its isolated subunits were characterized at 220 MHz and 22 degrees C. The spectra of ferric low spin derivatives of the isolated subunits were approximately superimposable on the corresponding hemoglobin spectra. On the other hand, the high spin spectra of the isolated subunits were greatly different from each other. The spectral anomaly in the ferric high spin complexes of the isolated beta subunit were interpreted to indicate other structural change than the hemichrome formation in the beta heme pocket. Difference in the subunit association effect between the high and low spin complexes of the isolated beta subunit was interpreted on the basis of a conformational change of the apoprotein dependent on the spin state of the beta heme iron."}
{"id": "PMID:479182", "title": "Formation of stable anhydrides from CoA transferase and hydroxamic acids.", "content": "Acetohydroxamic acid reacts with the enzyme-CoA form of succinyl-CoA:3-ketoacid coenzyme A transferase to give an inactive product with a rate constant of 860 M-1 min-1 at pH 8.1, 25 degrees C. The reaction is reversible in the presence of coenzyme A and has an equilibrium constant of 0.040. The product is an anhydride that is an analog of the intermediate that has been postulated in the normal catalytic pathway; it is inactive because coenzyme A does not react with the acyl group of the hydroxamic acid. The equilibrium constant for formation of the anhydride from the thil ester of enzyme and methyl 3-mercaptopropionate is 75 times larger than the equilibrium constant of 2.2 for the formation of N,O-diacetylhydroxylamine from acetohydroxamic acid and acetyl-CoA. This shows that the enzyme stabilizes the anhydride at the active site by at least -2.6 kcal mol-1. Succinomonohydroxamic acid reacts with enzyme-CoA as both a substrate and an inactivator, with relative rate constants of 25:1. The inactivation is irreversible, indicating that the enzyme provides a larger stabilization of at least -5.9 kcal mol-1 for the anhydride of an analog of the specific substrate, succinate. The results are consistent with the hypothesis that the enzyme stabilizes an anhydride that is formed at the active site during turnover of normal substrates through a stepwise reaction mechanism.", "contents": "Formation of stable anhydrides from CoA transferase and hydroxamic acids. Acetohydroxamic acid reacts with the enzyme-CoA form of succinyl-CoA:3-ketoacid coenzyme A transferase to give an inactive product with a rate constant of 860 M-1 min-1 at pH 8.1, 25 degrees C. The reaction is reversible in the presence of coenzyme A and has an equilibrium constant of 0.040. The product is an anhydride that is an analog of the intermediate that has been postulated in the normal catalytic pathway; it is inactive because coenzyme A does not react with the acyl group of the hydroxamic acid. The equilibrium constant for formation of the anhydride from the thil ester of enzyme and methyl 3-mercaptopropionate is 75 times larger than the equilibrium constant of 2.2 for the formation of N,O-diacetylhydroxylamine from acetohydroxamic acid and acetyl-CoA. This shows that the enzyme stabilizes the anhydride at the active site by at least -2.6 kcal mol-1. Succinomonohydroxamic acid reacts with enzyme-CoA as both a substrate and an inactivator, with relative rate constants of 25:1. The inactivation is irreversible, indicating that the enzyme provides a larger stabilization of at least -5.9 kcal mol-1 for the anhydride of an analog of the specific substrate, succinate. The results are consistent with the hypothesis that the enzyme stabilizes an anhydride that is formed at the active site during turnover of normal substrates through a stepwise reaction mechanism."}
{"id": "PMID:479184", "title": "Enzymic memory. Steady state kinetic and physical studies with ascorbate oxidase and aspartate aminotransferase.", "content": "Enzymic memory is a kinetic phenomenon observable in double displacement mechanisms. The defining feature of enzymic memory is the occurrence of different rates of transfer for a common transferable group from the substituted enzymes obtained with different donor substrates. Memory behavior was previously demonstrated for both the bovine and human liver rhodaneses (EC 2.8.1.1). Steady state kinetic tests for enzymic memory have now been done with ascorbate oxidase (EC 1.10.3.3) and aspartate aminotransferase (EC 2.6.1.1). The results were positive with ascorbate oxidase, which showed an oxygen reactivity ratio of 1:20:300 for the reduced enzymes obtained with reductate, araboascorbate, and ascorbate, respectively. Results were negative for the aminotransferase tested with the alternate donors glutamate and cysteine sulfinate, with oxaloacetate as the common acceptor. The structural basis of the ascorbate oxidase results was probed by comparison of both the ultraviolet absorption and fluorescence spectra of the oxidized enzyme with those of the reduced forms obtained with ascorbate and reductate. The results are consistent with a conformational basis for the memory phenomenon.", "contents": "Enzymic memory. Steady state kinetic and physical studies with ascorbate oxidase and aspartate aminotransferase. Enzymic memory is a kinetic phenomenon observable in double displacement mechanisms. The defining feature of enzymic memory is the occurrence of different rates of transfer for a common transferable group from the substituted enzymes obtained with different donor substrates. Memory behavior was previously demonstrated for both the bovine and human liver rhodaneses (EC 2.8.1.1). Steady state kinetic tests for enzymic memory have now been done with ascorbate oxidase (EC 1.10.3.3) and aspartate aminotransferase (EC 2.6.1.1). The results were positive with ascorbate oxidase, which showed an oxygen reactivity ratio of 1:20:300 for the reduced enzymes obtained with reductate, araboascorbate, and ascorbate, respectively. Results were negative for the aminotransferase tested with the alternate donors glutamate and cysteine sulfinate, with oxaloacetate as the common acceptor. The structural basis of the ascorbate oxidase results was probed by comparison of both the ultraviolet absorption and fluorescence spectra of the oxidized enzyme with those of the reduced forms obtained with ascorbate and reductate. The results are consistent with a conformational basis for the memory phenomenon."}
{"id": "PMID:479186", "title": "A concanavalin A-resistant Chinese hamster ovary cell line is deficient in the synthesis of [3H]glucosyl oligosaccharide-lipid.", "content": "In this report we present an initial determination of the biochemical defect present in a Chinese hamster ovary cell line selected for resistance to concanavalin A. Membranes of this mutant, B211, incorporated at least 10-fold less mannose from GDP-[14C]mannose into oligosaccharide-lipid than membranes of the wild type. In the presence of dolichol phosphate, membranes of the mutant and wild type exhibited similar rates of synthesis of number of early intermediates, namely, mannosylphosphoryldolichol, N-acetylglucosaminyl- and N,N'-diacetylchitobiosylpyrophosphoryldolichol, glucosylphosphoryldolichol, and mannosyloligosaccharide-lipid. The membranes of B211 did not incorporate glucose from UDP-[3H]glucose into oligosaccharide-lipid or protein. Comparison by gel filtration chromatography of oligosaccharides derived from the oligosaccharide-lipids of B211 and wild type cells labeled with [2-3H]mannose revealed that B211 cells incorporated little if any label into an oligosaccharide corresponding to the most excluded oligosaccharide labeled by wild type cells. This concanavalin A-resistant cell line appears to lack the ability to glucosylate oligosaccharide-lipid.", "contents": "A concanavalin A-resistant Chinese hamster ovary cell line is deficient in the synthesis of [3H]glucosyl oligosaccharide-lipid. In this report we present an initial determination of the biochemical defect present in a Chinese hamster ovary cell line selected for resistance to concanavalin A. Membranes of this mutant, B211, incorporated at least 10-fold less mannose from GDP-[14C]mannose into oligosaccharide-lipid than membranes of the wild type. In the presence of dolichol phosphate, membranes of the mutant and wild type exhibited similar rates of synthesis of number of early intermediates, namely, mannosylphosphoryldolichol, N-acetylglucosaminyl- and N,N'-diacetylchitobiosylpyrophosphoryldolichol, glucosylphosphoryldolichol, and mannosyloligosaccharide-lipid. The membranes of B211 did not incorporate glucose from UDP-[3H]glucose into oligosaccharide-lipid or protein. Comparison by gel filtration chromatography of oligosaccharides derived from the oligosaccharide-lipids of B211 and wild type cells labeled with [2-3H]mannose revealed that B211 cells incorporated little if any label into an oligosaccharide corresponding to the most excluded oligosaccharide labeled by wild type cells. This concanavalin A-resistant cell line appears to lack the ability to glucosylate oligosaccharide-lipid."}
{"id": "PMID:479189", "title": "Correlation of ultrastructure of reconstituted sarcoplasmic reticulum membrane vesicles with variation in phospholipid to protein ratio.", "content": "We have previously described the reconstitution of functional membrane vesicles with lipid content similar to that of the normal sarcoplasmic reticulum membrane (approximately 1.0 mumol of phospholipid/mg of protein). The present study describes methodology to prepare reconstituted membrane vesicles with defined phospholipid to protein ratio, both lower and higher than that of the original membrane. The Ca2+ loading rate and efficiency are greatest in the membranes of highest protein content (0.38 mumol of phospholipid/mg of protein), decline slowly as the lipid content is quadrupled, and decrease markedly as the lipid content is quadrupled again. Such membranes of defined composition can be used to study lipid-protein interaction and to correlate membrane structure with composition. The number of particles observed by freeze-fracture electron microscopy can be correlated with protein content, whereas the percentage of smooth domain is proportional to the lipid content of the reconstituted membrane. Since 90% or more of the protein of the reconstituted membrane is the calcium pump protein, the number of particles observed by freeze-fracture is directly proportional to the amount of calcium pump protein in the membrane. The number of pump molecules calculated to be in the membrane is greater by a factor of two than the number of particles which we observed. This multiplicity ratio could be greater depending upon the assumptions made regarding the width of the membrane (see \"Appendix\"). Thus, it would appear that the particles consist of two or more molecules of pump protein. The change in protein concentration of the membrane is reflected also in thin sections and by negative staining. In thin sections, the broad inner and outer 70 A bands become discontinuous and patchy and, in the limit, approach a symmetrical 20,20,20 A trilayer as the protein content of the membrane becomes small. In an analogous fashion, the concentration of particles at the surface of the membrane, observed by negative staining, decreases with increasing lipid concentration in the membrane. Thus, the correlation of composition with structure can be observed by each of the three methods of sample preparation for electron microscopic analysis.", "contents": "Correlation of ultrastructure of reconstituted sarcoplasmic reticulum membrane vesicles with variation in phospholipid to protein ratio. We have previously described the reconstitution of functional membrane vesicles with lipid content similar to that of the normal sarcoplasmic reticulum membrane (approximately 1.0 mumol of phospholipid/mg of protein). The present study describes methodology to prepare reconstituted membrane vesicles with defined phospholipid to protein ratio, both lower and higher than that of the original membrane. The Ca2+ loading rate and efficiency are greatest in the membranes of highest protein content (0.38 mumol of phospholipid/mg of protein), decline slowly as the lipid content is quadrupled, and decrease markedly as the lipid content is quadrupled again. Such membranes of defined composition can be used to study lipid-protein interaction and to correlate membrane structure with composition. The number of particles observed by freeze-fracture electron microscopy can be correlated with protein content, whereas the percentage of smooth domain is proportional to the lipid content of the reconstituted membrane. Since 90% or more of the protein of the reconstituted membrane is the calcium pump protein, the number of particles observed by freeze-fracture is directly proportional to the amount of calcium pump protein in the membrane. The number of pump molecules calculated to be in the membrane is greater by a factor of two than the number of particles which we observed. This multiplicity ratio could be greater depending upon the assumptions made regarding the width of the membrane (see \"Appendix\"). Thus, it would appear that the particles consist of two or more molecules of pump protein. The change in protein concentration of the membrane is reflected also in thin sections and by negative staining. In thin sections, the broad inner and outer 70 A bands become discontinuous and patchy and, in the limit, approach a symmetrical 20,20,20 A trilayer as the protein content of the membrane becomes small. In an analogous fashion, the concentration of particles at the surface of the membrane, observed by negative staining, decreases with increasing lipid concentration in the membrane. Thus, the correlation of composition with structure can be observed by each of the three methods of sample preparation for electron microscopic analysis."}
{"id": "PMID:479190", "title": "Albumin-like proteins from the pituitary glands of Dahl salt-resistant rats.", "content": "Dahl selectively bred rats for susceptibility (S strain) or resistance (R strain) to the hypertensive effect of high salt (NaCl) diet. Pituitary glands of R rats accumulate large amounts of four unique proteins not seen in S rats. These proteins were called R1, R2, R3, and R4 in order of decreasing electrophoretic mobility. Albumin, R4, R2, and R1 all bound to an affinity column for albumin (Cibacron blue 3G-A dye coupled to agarose) and were eluted in that order by a KSCN gradient. It was shown by crossed immunoelectrophoresis that R1 and R2 cross-react with plasma albumin. Peptide maps or tryptic digest of R1 and albumin showed that the majority of peptides generated were identical. It was not possible to incorporate labeled amino acid into albumin or the albumin-like R proteins with pituitary incubates, indicating that albumin-like proteins were not synthesized de novo by pituitary glands. R rat pituitary glands showed much greater protease (arginine esterase) activity than did S. This suggests that R proteins are formed locally in the pituitary gland of R proteins are formed locally in the pituitary gland of R rats by cleavabe of specific peptide bonds in albumin. The function of these endogenous albumin fragments is unknown, but albumin fragments produced in vitro by other investigators are known to potentiate bradykinin, a hypotensive peptide.", "contents": "Albumin-like proteins from the pituitary glands of Dahl salt-resistant rats. Dahl selectively bred rats for susceptibility (S strain) or resistance (R strain) to the hypertensive effect of high salt (NaCl) diet. Pituitary glands of R rats accumulate large amounts of four unique proteins not seen in S rats. These proteins were called R1, R2, R3, and R4 in order of decreasing electrophoretic mobility. Albumin, R4, R2, and R1 all bound to an affinity column for albumin (Cibacron blue 3G-A dye coupled to agarose) and were eluted in that order by a KSCN gradient. It was shown by crossed immunoelectrophoresis that R1 and R2 cross-react with plasma albumin. Peptide maps or tryptic digest of R1 and albumin showed that the majority of peptides generated were identical. It was not possible to incorporate labeled amino acid into albumin or the albumin-like R proteins with pituitary incubates, indicating that albumin-like proteins were not synthesized de novo by pituitary glands. R rat pituitary glands showed much greater protease (arginine esterase) activity than did S. This suggests that R proteins are formed locally in the pituitary gland of R proteins are formed locally in the pituitary gland of R rats by cleavabe of specific peptide bonds in albumin. The function of these endogenous albumin fragments is unknown, but albumin fragments produced in vitro by other investigators are known to potentiate bradykinin, a hypotensive peptide."}
{"id": "PMID:479191", "title": "Fat cell protein phosphorylation. Identification of phosphoprotein-2 as ATP-citrate lyase.", "content": "We have purified to apparent homogeneity a phosphoprotein from rat adipose tissue which is rapidly phosphorylated in vitro by ATP. The native phosphoprotein has an approximate sedimentation coefficient of 14.8 S. On sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, the protein dissociated into identical subunits of Mr = 128,000. A phosphoprotein with similar properties was also isolated from liver. Purified phosphoproteins from fat cells and liver had ATP-citrate lyase activity and co-migrated on sodium dodecyl sulfate gels with fat cell phosphoprotein-2, the phosphorylation of which is increased by incubating fat cells with insulin. The phosphoamino acid residue of the cells with insulin. The phosphoamino acid residue of the phosphoprotein was identified as tau-phosphohistidine. These evidences suggest that fat cell phosphoprotein-2 is ATP-citrate lyase.", "contents": "Fat cell protein phosphorylation. Identification of phosphoprotein-2 as ATP-citrate lyase. We have purified to apparent homogeneity a phosphoprotein from rat adipose tissue which is rapidly phosphorylated in vitro by ATP. The native phosphoprotein has an approximate sedimentation coefficient of 14.8 S. On sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, the protein dissociated into identical subunits of Mr = 128,000. A phosphoprotein with similar properties was also isolated from liver. Purified phosphoproteins from fat cells and liver had ATP-citrate lyase activity and co-migrated on sodium dodecyl sulfate gels with fat cell phosphoprotein-2, the phosphorylation of which is increased by incubating fat cells with insulin. The phosphoamino acid residue of the cells with insulin. The phosphoamino acid residue of the phosphoprotein was identified as tau-phosphohistidine. These evidences suggest that fat cell phosphoprotein-2 is ATP-citrate lyase."}
{"id": "PMID:479192", "title": "The topological orientation of N,N'-diacetylchitobiosylpyrophosphoryldolichol in artificial and natural membranes.", "content": "Purified N,N'-diacetylchitobiosylpyrophosphoryldolichol (chitobiosyl-lipid) in the presence of detergent was shown to act as a substrate for the soluble enzyme galactosyltransferase. The nature of this transfer reaction and the galactose-containing trisaccharide-lipid product have been partially characterized. Using galactosyltransferase as a probe, the topolical arrangement of chitobiosyl-lipid in both artificial and natural membranes has been examined. When incorporated into unilamellar liposomes made from phosphatidylcholine, the disaccharide residue of chitogiosyl-lipid adopts a random transbilayer orientation. Furthermore, no significant mobility in the transverse plane of the membrane (i.e. flip-flop) is detectable. Using both sealed total microsomes or isolated rough microsomes from hen oviduct, the topology of chitobiosyl-lipid after its biosynthesis in the membrane has been determined. The results suggest that, once formed, chitobiosyl-lipid is a relatively static component of the membrane and is oriented with its disaccharide residue facing the lumen of the endoplasmic reticulum.", "contents": "The topological orientation of N,N'-diacetylchitobiosylpyrophosphoryldolichol in artificial and natural membranes. Purified N,N'-diacetylchitobiosylpyrophosphoryldolichol (chitobiosyl-lipid) in the presence of detergent was shown to act as a substrate for the soluble enzyme galactosyltransferase. The nature of this transfer reaction and the galactose-containing trisaccharide-lipid product have been partially characterized. Using galactosyltransferase as a probe, the topolical arrangement of chitobiosyl-lipid in both artificial and natural membranes has been examined. When incorporated into unilamellar liposomes made from phosphatidylcholine, the disaccharide residue of chitogiosyl-lipid adopts a random transbilayer orientation. Furthermore, no significant mobility in the transverse plane of the membrane (i.e. flip-flop) is detectable. Using both sealed total microsomes or isolated rough microsomes from hen oviduct, the topology of chitobiosyl-lipid after its biosynthesis in the membrane has been determined. The results suggest that, once formed, chitobiosyl-lipid is a relatively static component of the membrane and is oriented with its disaccharide residue facing the lumen of the endoplasmic reticulum."}
{"id": "PMID:479194", "title": "Quaternary structure of pyruvate carboxylase from Pseudomonas citronellolis.", "content": "Physical-chemical studies of pyruvate carboxylase from Pseudomonas citronellolis demonstrate that the enzyme has an alpha 4 beta 4 structure. The individual polypeptides, alpha (Mr = 65,000) and beta (Mr = 54,000), were separated and isolated by preparative gel electrophoresis. Analysis of the relationship between Coomassie blue staining and protein quantity for each polypeptide indicated that the alpha and beta subunits are present in a 1:1 stoichiometry in the native enzyme. Determinations of the molecular weight of the protein by sedimentation equilibrium (Mr = 454,000), gel filtration analysis (Mr = 510,000), disc gel electrophoresis (Mr = 530,000), and mass measurement from the Scanning Transmission Electron Microscope (Mr = 530,000) are consistent with the proposed alpha 4 beta 4 structure. Disc gel electrophoresis studies revealed that under certain circumstances the enzyme may dissociate to a smaller molecular weight species (Mr = 228,000). This dissociation phenomenon could explain the earlier reported observation of Taylor et al. ((1972) J. Biol. Chem 22, 7388-8390) that the enzyme had a molecular weight of 265,000. Evidence from electron microscopic studies shows that the three-dimensional structure of this enzyme is quite distinct from other species of pyruvate carboxylase. The enzyme does not show the typical rhombic appearance which has been noted for chicken liver, sheep liver, and yeast pyruvate carboxylase.", "contents": "Quaternary structure of pyruvate carboxylase from Pseudomonas citronellolis. Physical-chemical studies of pyruvate carboxylase from Pseudomonas citronellolis demonstrate that the enzyme has an alpha 4 beta 4 structure. The individual polypeptides, alpha (Mr = 65,000) and beta (Mr = 54,000), were separated and isolated by preparative gel electrophoresis. Analysis of the relationship between Coomassie blue staining and protein quantity for each polypeptide indicated that the alpha and beta subunits are present in a 1:1 stoichiometry in the native enzyme. Determinations of the molecular weight of the protein by sedimentation equilibrium (Mr = 454,000), gel filtration analysis (Mr = 510,000), disc gel electrophoresis (Mr = 530,000), and mass measurement from the Scanning Transmission Electron Microscope (Mr = 530,000) are consistent with the proposed alpha 4 beta 4 structure. Disc gel electrophoresis studies revealed that under certain circumstances the enzyme may dissociate to a smaller molecular weight species (Mr = 228,000). This dissociation phenomenon could explain the earlier reported observation of Taylor et al. ((1972) J. Biol. Chem 22, 7388-8390) that the enzyme had a molecular weight of 265,000. Evidence from electron microscopic studies shows that the three-dimensional structure of this enzyme is quite distinct from other species of pyruvate carboxylase. The enzyme does not show the typical rhombic appearance which has been noted for chicken liver, sheep liver, and yeast pyruvate carboxylase."}
{"id": "PMID:479195", "title": "Purification of the glucocorticoid receptor from rat liver cytosol.", "content": "The [3H]-triamcinolone acetonide-labeled glucocorticoid receptor from rat liver cytosol was purified to 85% homogeneity according to sodium dodecyl sulfate gel electrophoresis. It consisted of one subunit with a molecular weight of 89,000 and had one ligand-binding site per molecule. The purification involved sequential chromatography on phosphocellulose, DNA-cellulose twice, and Sephadex G-200. Between the two chromatography steps on DNA-cellulose, the receptor was heat activated. The receptor was affinity eluted from the second DNA-cellulose column with pyrodixal 5'-phosphate. The purification achieved in the first three chromatographic steps varied between 60 and 95% homogeneity in different experiments. After chromatography on the second DNA-cellulose column, the steroid.receptor complex had a Stokes radius of 6.0 nm and a sedimentation coefficient of 3.4 S in 0.15 M KCl. In the absence of KCl, the sedimentation coefficient was 3.6 S. After concentration on hydroxylapatite, the steroid.receptor complex was analyzed by isoelectric focusing in polyacrylamide gel. The radioactivity was shown to focus together with the major protein band with pI 5.8. Following limited proteolysis with trypsin, the radioactivity, together with the major protein band, focused at pI 6.2 as previously described for the unpurified steroid.receptor complex.", "contents": "Purification of the glucocorticoid receptor from rat liver cytosol. The [3H]-triamcinolone acetonide-labeled glucocorticoid receptor from rat liver cytosol was purified to 85% homogeneity according to sodium dodecyl sulfate gel electrophoresis. It consisted of one subunit with a molecular weight of 89,000 and had one ligand-binding site per molecule. The purification involved sequential chromatography on phosphocellulose, DNA-cellulose twice, and Sephadex G-200. Between the two chromatography steps on DNA-cellulose, the receptor was heat activated. The receptor was affinity eluted from the second DNA-cellulose column with pyrodixal 5'-phosphate. The purification achieved in the first three chromatographic steps varied between 60 and 95% homogeneity in different experiments. After chromatography on the second DNA-cellulose column, the steroid.receptor complex had a Stokes radius of 6.0 nm and a sedimentation coefficient of 3.4 S in 0.15 M KCl. In the absence of KCl, the sedimentation coefficient was 3.6 S. After concentration on hydroxylapatite, the steroid.receptor complex was analyzed by isoelectric focusing in polyacrylamide gel. The radioactivity was shown to focus together with the major protein band with pI 5.8. Following limited proteolysis with trypsin, the radioactivity, together with the major protein band, focused at pI 6.2 as previously described for the unpurified steroid.receptor complex."}
{"id": "PMID:479196", "title": "ATP-induced endocytosis in human erythrocyte ghosts. Characterization of the process and isolation of the endocytosed vesicles.", "content": "ATP-induced endocytosis in human erythrocyte ghosts has been studied, and a procedure for the isolation of the endocytotic vesicles is described. Under isotonic conditions and 37 degrees C, optimal endocytosis occurs with concentrations of 4 to 10 mM MgATP. Within 30 min, up to 45% of the membrane is removed from the surface and converted into sealed inside-out vesicles. Local anesthetics, such as chlorpromazine, potentiate ATP-induced endocytosis in ghosts. Forcing cells containing endocytotic vesicles through a hypodermic needle leads to the exclusive fragmentation of the outermost plasma membrane. The endocytosed vesicles can then be separated from these fragments by centrifugation on a gradient of dextran T70. Biochemical analyses indicate that endocytotic vesicles contain full complements of the major membrane proteins (i.e. also spectrin and actin), common phospholipids, fatty acids, and cholesterol. Furthermore, they exhibit a fully intact spectrin component 2 phosphorylation machinery. In contrast, MgATPase activity is largely excluded from these vesicles. The novel inside-out vesicles described have properties different from those of previously analyzed fragments of the erythrocyte membrane. They will permit a detailed study of a native spectrin-actin network now exposed to the outside.", "contents": "ATP-induced endocytosis in human erythrocyte ghosts. Characterization of the process and isolation of the endocytosed vesicles. ATP-induced endocytosis in human erythrocyte ghosts has been studied, and a procedure for the isolation of the endocytotic vesicles is described. Under isotonic conditions and 37 degrees C, optimal endocytosis occurs with concentrations of 4 to 10 mM MgATP. Within 30 min, up to 45% of the membrane is removed from the surface and converted into sealed inside-out vesicles. Local anesthetics, such as chlorpromazine, potentiate ATP-induced endocytosis in ghosts. Forcing cells containing endocytotic vesicles through a hypodermic needle leads to the exclusive fragmentation of the outermost plasma membrane. The endocytosed vesicles can then be separated from these fragments by centrifugation on a gradient of dextran T70. Biochemical analyses indicate that endocytotic vesicles contain full complements of the major membrane proteins (i.e. also spectrin and actin), common phospholipids, fatty acids, and cholesterol. Furthermore, they exhibit a fully intact spectrin component 2 phosphorylation machinery. In contrast, MgATPase activity is largely excluded from these vesicles. The novel inside-out vesicles described have properties different from those of previously analyzed fragments of the erythrocyte membrane. They will permit a detailed study of a native spectrin-actin network now exposed to the outside."}
{"id": "PMID:479197", "title": "Structure of the major gangliosides from bovine thyroid.", "content": "After preparative isolation, the carbohydrate, long chain base, and fatty acid composition of the major gangliosides from bovine thyroid have been analyzed. The structures were elucidated by determining the molar ratio of the building blocks, permethylation analysis, and enzymatic degradation studies. The following structures are identified: N-Acetylneuraminyl(2,3)-galactosyl(1,4)glucosyl(1,1)ceramidie; N-glycolyneuraminyl(2,3)galactosyl(1,4)glucosyl(1,1)ceramide; galactosyl(1,3)N-acetylgalactosaminyl[(3,2)N-acetylneuraminyl](1,4)galactosyl(1,4)glucosyl(1,1)ceramide; fucosyl(1,2)galactosyl(1,3)N-acetylgalactosaminyl[(3,2)N-acetylneuraminyl](1,4)galactosyl(1,4)glucosyl(1,1)-ceramide. The structures were confirmed by direct inlet mass spectrometry of the permethylated gangliosides and the corresponding asialo derivatives. Structures are proposed for common ions in the different mass spectra.", "contents": "Structure of the major gangliosides from bovine thyroid. After preparative isolation, the carbohydrate, long chain base, and fatty acid composition of the major gangliosides from bovine thyroid have been analyzed. The structures were elucidated by determining the molar ratio of the building blocks, permethylation analysis, and enzymatic degradation studies. The following structures are identified: N-Acetylneuraminyl(2,3)-galactosyl(1,4)glucosyl(1,1)ceramidie; N-glycolyneuraminyl(2,3)galactosyl(1,4)glucosyl(1,1)ceramide; galactosyl(1,3)N-acetylgalactosaminyl[(3,2)N-acetylneuraminyl](1,4)galactosyl(1,4)glucosyl(1,1)ceramide; fucosyl(1,2)galactosyl(1,3)N-acetylgalactosaminyl[(3,2)N-acetylneuraminyl](1,4)galactosyl(1,4)glucosyl(1,1)-ceramide. The structures were confirmed by direct inlet mass spectrometry of the permethylated gangliosides and the corresponding asialo derivatives. Structures are proposed for common ions in the different mass spectra."}
{"id": "PMID:479198", "title": "Structural characterization of lactotetraosylceramide, a novel glycosphingolipid isolated from human meconium.", "content": "In the course of work on a systematic structural mapping of nonacid glycosphingolipids of human meconia, special attention was given to a major component preliminarily identified as an isomer of neolactotetraosylceramide (paragloboside). This component was isolated in its pure form from meconium of a blood group O individual and subjected to detailed structural analyses, using mass spectrometry and proton NMR spectroscopy on intact permethylated and permethylated-reduced (LiAlH4) derivatives, and gas liquid chromatography on degradational products of native, permethylated, and permethylated-reduced derivatives. The isolated compound was conclusively shown to have the structure Galp beta 1 yields 3GlcNAcp beta 1 yields 3Galp beta 1 yields 4Glcp beta 1 yields 1Cer, and is thus identified as lactotetraosylceramide. The major fatty acids were 2-hydroxy fatty acids with 16 and 20 to 24 carbon atoms, and the bases were sphingosine and phytosphingosine. This glycolipid, although not isolated and structurally characterized before, has long been thought of as a precursor substance of the Lewis active glycolipids and of ABH-active glycolipids with a type 1 saccharide chain.", "contents": "Structural characterization of lactotetraosylceramide, a novel glycosphingolipid isolated from human meconium. In the course of work on a systematic structural mapping of nonacid glycosphingolipids of human meconia, special attention was given to a major component preliminarily identified as an isomer of neolactotetraosylceramide (paragloboside). This component was isolated in its pure form from meconium of a blood group O individual and subjected to detailed structural analyses, using mass spectrometry and proton NMR spectroscopy on intact permethylated and permethylated-reduced (LiAlH4) derivatives, and gas liquid chromatography on degradational products of native, permethylated, and permethylated-reduced derivatives. The isolated compound was conclusively shown to have the structure Galp beta 1 yields 3GlcNAcp beta 1 yields 3Galp beta 1 yields 4Glcp beta 1 yields 1Cer, and is thus identified as lactotetraosylceramide. The major fatty acids were 2-hydroxy fatty acids with 16 and 20 to 24 carbon atoms, and the bases were sphingosine and phytosphingosine. This glycolipid, although not isolated and structurally characterized before, has long been thought of as a precursor substance of the Lewis active glycolipids and of ABH-active glycolipids with a type 1 saccharide chain."}
{"id": "PMID:479215", "title": "Characteristics of tissue growth into Proplast and porous polyethylene implants in bone.", "content": "(1) Bone does not form within internal pores of undistorted Proplast implants because of the small interconnecting pore size of the material; (2) the nonosseous, fibrous tissue which exists in the pores of Proplast implants in bone is not attached to the surrounding bone (i.e., Sharpey's fibers are not present). The load-bearing support which can be afforded by Proplast implants is limited by the incomplete bone ingrowth along the margins of the material and the tensile strength of Proplast.", "contents": "Characteristics of tissue growth into Proplast and porous polyethylene implants in bone. (1) Bone does not form within internal pores of undistorted Proplast implants because of the small interconnecting pore size of the material; (2) the nonosseous, fibrous tissue which exists in the pores of Proplast implants in bone is not attached to the surrounding bone (i.e., Sharpey's fibers are not present). The load-bearing support which can be afforded by Proplast implants is limited by the incomplete bone ingrowth along the margins of the material and the tensile strength of Proplast."}
{"id": "PMID:479216", "title": "Behavior of three high-Cu amalgams.", "content": "Amalgams made from three single-composition high-Cu alloys of the same nominal composition were investigated for differences in creep behavior. The curves of creep vs. final Hg content for all three alloys showed a sudden creep increase (jump) at approximately 46% final Hg content. Previous studies have shown this creep jump to be associated with the initiation of the Sn--Hg phase (gamma 2). The anticipated final Hg content in clinical restorations for two of these alloys is below the creep jump and gamma 2 would not be expected. On the other hand, the clinical Hg content for the third alloy was above its creep jump and gamma 2 would be expected. The effect on clinical behavior of this finding require further investigation.", "contents": "Behavior of three high-Cu amalgams. Amalgams made from three single-composition high-Cu alloys of the same nominal composition were investigated for differences in creep behavior. The curves of creep vs. final Hg content for all three alloys showed a sudden creep increase (jump) at approximately 46% final Hg content. Previous studies have shown this creep jump to be associated with the initiation of the Sn--Hg phase (gamma 2). The anticipated final Hg content in clinical restorations for two of these alloys is below the creep jump and gamma 2 would not be expected. On the other hand, the clinical Hg content for the third alloy was above its creep jump and gamma 2 would be expected. The effect on clinical behavior of this finding require further investigation."}
{"id": "PMID:479217", "title": "In vitro blood compatibility of glycosaminoglycan-precipitated collagens.", "content": "Precipitation of bovine hide collagen by chondroitin 6-sulfate at low pH and subsequent crosslinking enhances the blood compatibility of native collagen. Both dehydrothermal crosslinking and complexation with chrondroitin 6-sulfate separately decrease the platelet-aggregating activity of collagen. Crosslinking also decreases the number of free acidic and free basic residues on collagen, which suggests that crosslinking involves these residues in condensation reactions with formation of intrachain and interchain synthetic peptide bonds. Clotting times for collagen precipitated with chondroitin 6-sulfate indicate that this surface does not activate or interfere with coagulation via either the intrinsic or extrinsic pathway. These findings support further consideration of collagen modified by chondroitin 6-sulfate as a blood compatible material.", "contents": "In vitro blood compatibility of glycosaminoglycan-precipitated collagens. Precipitation of bovine hide collagen by chondroitin 6-sulfate at low pH and subsequent crosslinking enhances the blood compatibility of native collagen. Both dehydrothermal crosslinking and complexation with chrondroitin 6-sulfate separately decrease the platelet-aggregating activity of collagen. Crosslinking also decreases the number of free acidic and free basic residues on collagen, which suggests that crosslinking involves these residues in condensation reactions with formation of intrachain and interchain synthetic peptide bonds. Clotting times for collagen precipitated with chondroitin 6-sulfate indicate that this surface does not activate or interfere with coagulation via either the intrinsic or extrinsic pathway. These findings support further consideration of collagen modified by chondroitin 6-sulfate as a blood compatible material."}
{"id": "PMID:479218", "title": "SEM fractography studies of porous vitreous carbon: a candidate biomaterial.", "content": "A new porous vitreous carbon material under development for use in orthopedic applications was investigated. Specimens were machined to appropriate sizes and fractured in one of the following modes: compression, cantilevered bending, or axial torsion. Scanning electron microscopy (SEM) was used to examine surface and internal features. Characteristics of a brittle, glassy material were noted. Findings included internal voids which appeared as craters, patches of whiskerlike fibrils, and edge impurities. Numerous microcracks caused by mechanical shaping and handling were the most remarkable structural defects. Pore channels which would allow bony ingrowth ranged in size from 50--500 micrometers with the majority between 200 and 300 micrometers. This study of porous vitreous carbon points to the need for stricter quality control in manufacturing, alternative methods for shaping and handling, and careful consideration in design and usage of a brittle material with marginal limits of safety for biomedical applications.", "contents": "SEM fractography studies of porous vitreous carbon: a candidate biomaterial. A new porous vitreous carbon material under development for use in orthopedic applications was investigated. Specimens were machined to appropriate sizes and fractured in one of the following modes: compression, cantilevered bending, or axial torsion. Scanning electron microscopy (SEM) was used to examine surface and internal features. Characteristics of a brittle, glassy material were noted. Findings included internal voids which appeared as craters, patches of whiskerlike fibrils, and edge impurities. Numerous microcracks caused by mechanical shaping and handling were the most remarkable structural defects. Pore channels which would allow bony ingrowth ranged in size from 50--500 micrometers with the majority between 200 and 300 micrometers. This study of porous vitreous carbon points to the need for stricter quality control in manufacturing, alternative methods for shaping and handling, and careful consideration in design and usage of a brittle material with marginal limits of safety for biomedical applications."}
{"id": "PMID:479219", "title": "Microelectrode studies of stress-generated potentials in four-point bending of bone.", "content": "A microelectrode technique has been developed to enable the study of stress-generated potentials (SGP) in bone to a spatial resolution of 5 micrometers. The technique has been used to measure the electrical potentials as a function of bone micromorphology in four-point bending. Electric fields ranging from 30 to 10(3) times greater than is measured by conventional macroscopic methods have been discovered at the Haversian canals for human and bovine cortical bone. The amplitude and direction of the electric field in the osteons depend specifically upon the amplitude and the sign (i.e., compression or tension) of the stress. The implications of this finding with regard to the origin of SGP and their possible physiological significance are considered.", "contents": "Microelectrode studies of stress-generated potentials in four-point bending of bone. A microelectrode technique has been developed to enable the study of stress-generated potentials (SGP) in bone to a spatial resolution of 5 micrometers. The technique has been used to measure the electrical potentials as a function of bone micromorphology in four-point bending. Electric fields ranging from 30 to 10(3) times greater than is measured by conventional macroscopic methods have been discovered at the Haversian canals for human and bovine cortical bone. The amplitude and direction of the electric field in the osteons depend specifically upon the amplitude and the sign (i.e., compression or tension) of the stress. The implications of this finding with regard to the origin of SGP and their possible physiological significance are considered."}
{"id": "PMID:479220", "title": "Microelectrode study of stress-generated potentials obtained from uniform and nonuniform compression of human bone.", "content": "By use of a previously developed microelectrode technique, the effect of nonuniform stresses on the stress-generated potentials (SGP) in bone were studied to a resolution of 5 micrometers. Comparison was made between uniformly and nonuniformly applied compression of human cortical bone. It was found that the radial electric fields for osteons in a specimen under uniform compression were equivalent, and such specimens possessed no macroscopic SGP; for nonuniform compression, the electric fields of osteons differed, and a macroscopic SGP was measured. The magnitude of the macroscopic SGP thus appears to be dependent upon local stress differences and, hence, on the SGP of local regions.", "contents": "Microelectrode study of stress-generated potentials obtained from uniform and nonuniform compression of human bone. By use of a previously developed microelectrode technique, the effect of nonuniform stresses on the stress-generated potentials (SGP) in bone were studied to a resolution of 5 micrometers. Comparison was made between uniformly and nonuniformly applied compression of human cortical bone. It was found that the radial electric fields for osteons in a specimen under uniform compression were equivalent, and such specimens possessed no macroscopic SGP; for nonuniform compression, the electric fields of osteons differed, and a macroscopic SGP was measured. The magnitude of the macroscopic SGP thus appears to be dependent upon local stress differences and, hence, on the SGP of local regions."}
{"id": "PMID:479221", "title": "Platelet interaction with synthetic copolypeptide films.", "content": "Kinetic and equilibrium studies of blood platelet binding to copolypeptide films show that attachment and serotonin release are not dependent upon the composition of the copolypeptide. Data may be explained by postulating that platelets frequently collide elastically with the surface but leave behind material that modifies subsequent behavior. Similarly, material released from platelets adsorbs at the interface and the extent of attachment and serotonin release are modified and controlled by these adsorbed species. Basically, if the platelet is exposed to a clean surface, its collision with the surface leads to activation and release. In the presence of inert protein, the collision is cushioned by the protein and platelets do not attach or release to any extent. Finally, if protein (or other entities) released from the platelet provide attachment sites, then attachment occurs without release. It is postulated that the behavior of platelets at surfaces is controlled by these interrelated processes.", "contents": "Platelet interaction with synthetic copolypeptide films. Kinetic and equilibrium studies of blood platelet binding to copolypeptide films show that attachment and serotonin release are not dependent upon the composition of the copolypeptide. Data may be explained by postulating that platelets frequently collide elastically with the surface but leave behind material that modifies subsequent behavior. Similarly, material released from platelets adsorbs at the interface and the extent of attachment and serotonin release are modified and controlled by these adsorbed species. Basically, if the platelet is exposed to a clean surface, its collision with the surface leads to activation and release. In the presence of inert protein, the collision is cushioned by the protein and platelets do not attach or release to any extent. Finally, if protein (or other entities) released from the platelet provide attachment sites, then attachment occurs without release. It is postulated that the behavior of platelets at surfaces is controlled by these interrelated processes."}
{"id": "PMID:479222", "title": "Degradation resistance of some candidate composite biomaterials.", "content": "The degradation resistance of matrix, fiber and composite systems which we have been studying as candidate orthopedic materials has been examined in two appropriate environments. Both resistance to steam sterilization in an autoclave environment and resistance to a simulated physiologic solution have been studied. In the autoclave study, samples were placed in a pressure cooker at 123 degrees C for differing amounts of time and tested for retention of mechanical properties. Results indicate that most of the materials tested could be autoclaved several times, as long as autoclave times did not exceed 1 hr. Longer autoclave times result in an accelerated degradation and loss of strength of all materials except the polypropylene. Polysulfone degrades after even the shortest autoclave duration. Resistance to the simulated physiologic environment was tested by measured retention of mechanical properties after immersion times in pseudo-extracellular fluid (PECF) at 37 degrees C for as long as three years. None of the materials showed any significant changes in properties after immersion in the PECF.", "contents": "Degradation resistance of some candidate composite biomaterials. The degradation resistance of matrix, fiber and composite systems which we have been studying as candidate orthopedic materials has been examined in two appropriate environments. Both resistance to steam sterilization in an autoclave environment and resistance to a simulated physiologic solution have been studied. In the autoclave study, samples were placed in a pressure cooker at 123 degrees C for differing amounts of time and tested for retention of mechanical properties. Results indicate that most of the materials tested could be autoclaved several times, as long as autoclave times did not exceed 1 hr. Longer autoclave times result in an accelerated degradation and loss of strength of all materials except the polypropylene. Polysulfone degrades after even the shortest autoclave duration. Resistance to the simulated physiologic environment was tested by measured retention of mechanical properties after immersion times in pseudo-extracellular fluid (PECF) at 37 degrees C for as long as three years. None of the materials showed any significant changes in properties after immersion in the PECF."}
{"id": "PMID:479223", "title": "Release characteristics of dibucaine dispersed in konjac gels.", "content": "A possible use of konjac gel for sustained release of drugs was examined in a monolithic system containing dibucaine. Dibucaine was dispersed in the gel which was prepared by gelation of the konjac flour in a borax solution at 60 degrees. The cumulative amount of the drug released plotted against the square root of time was linear in the monolithic system. This relationship was in agreement with that expected from the theoretical equation for planar configuration. The mechanism of the release of the drug from the gel may be considered to be leaching of the drug by the permeating fluid. The release profile from dried konjac gel was similar to that from undried gel, but that from unwarmed gel showed a deviation from linearity although sustained release was similarly obtained.", "contents": "Release characteristics of dibucaine dispersed in konjac gels. A possible use of konjac gel for sustained release of drugs was examined in a monolithic system containing dibucaine. Dibucaine was dispersed in the gel which was prepared by gelation of the konjac flour in a borax solution at 60 degrees. The cumulative amount of the drug released plotted against the square root of time was linear in the monolithic system. This relationship was in agreement with that expected from the theoretical equation for planar configuration. The mechanism of the release of the drug from the gel may be considered to be leaching of the drug by the permeating fluid. The release profile from dried konjac gel was similar to that from undried gel, but that from unwarmed gel showed a deviation from linearity although sustained release was similarly obtained."}
{"id": "PMID:479227", "title": "Resistant congenital club foot--one-stage posteromedial release with internal fixation. A follow-up report of a fifteen-year experience.", "content": "I have reviewed my personal experience with 240 resistant congenital club feet (176 patients) treated surgically by one-stage posteromedial release with internal fixation. The end results in the 149 feet that had a follow-up of two to fifteen years were: excellent or good, 83.8 per cent; fair, 10.7 per cent; and failures, 5.3 per cent. The best results with the least complications were in children who were operated on between the ages of one and two years. A statistical analysis of fifty-three feet with a follow-up of less than two years is reported for comparison with the results after longer follow-up to allow analysis of all the known failures and complications that have been encountered with the operation. Possible causes and prevention of fair and poor results are discussed. Modifications of the technique, which originally was reported in 1971, are described.", "contents": "Resistant congenital club foot--one-stage posteromedial release with internal fixation. A follow-up report of a fifteen-year experience. I have reviewed my personal experience with 240 resistant congenital club feet (176 patients) treated surgically by one-stage posteromedial release with internal fixation. The end results in the 149 feet that had a follow-up of two to fifteen years were: excellent or good, 83.8 per cent; fair, 10.7 per cent; and failures, 5.3 per cent. The best results with the least complications were in children who were operated on between the ages of one and two years. A statistical analysis of fifty-three feet with a follow-up of less than two years is reported for comparison with the results after longer follow-up to allow analysis of all the known failures and complications that have been encountered with the operation. Possible causes and prevention of fair and poor results are discussed. Modifications of the technique, which originally was reported in 1971, are described."}
{"id": "PMID:479228", "title": "Serratus anterior paralysis in the young athlete.", "content": "Ten cases of isolated, complete paralysis of the serratus anterior muscle were diagnosed in young athletes during a three-year period. One patient had recurrent partial paralysis of the serratus anterior muscle, the first such case reported. From studies on cadavera and clinical observations, we concluded that paralysis of the serratus anterior muscle results from a traction injury to the long thoracic nerve of Bell. Since full recovery usually occurs in an average of nine months, surgical methods of treatment should be reserved for patients in whom function fails to return after a two-year period. Non-strenuous use of the involved extremity with avoidance of the precipitating activity, followed by exercises designed to maintain the range of motion of the shoulder and to increase the strength of associated muscles, is advocated for treatment of acute or repetitive injuries to the long thoracic nerve of Bell.", "contents": "Serratus anterior paralysis in the young athlete. Ten cases of isolated, complete paralysis of the serratus anterior muscle were diagnosed in young athletes during a three-year period. One patient had recurrent partial paralysis of the serratus anterior muscle, the first such case reported. From studies on cadavera and clinical observations, we concluded that paralysis of the serratus anterior muscle results from a traction injury to the long thoracic nerve of Bell. Since full recovery usually occurs in an average of nine months, surgical methods of treatment should be reserved for patients in whom function fails to return after a two-year period. Non-strenuous use of the involved extremity with avoidance of the precipitating activity, followed by exercises designed to maintain the range of motion of the shoulder and to increase the strength of associated muscles, is advocated for treatment of acute or repetitive injuries to the long thoracic nerve of Bell."}
{"id": "PMID:479229", "title": "Tennis elbow. The surgical treatment of lateral epicondylitis.", "content": "Of the 1,213 clinical cases of lateral tennis elbow seen during the time period from December 19, 1971, to October 31, 1977, eighty-eight elbows in eighty-two patients had operative treatment. The lesion that was consistently identified at surgery was immature fibroblastic and vascular infiltration of the origin of the extensor carpi radialis brevis. A specific surgical technique was employed, including exposure of the extensor carpi radialis brevis, excision of the identified lesion, and repair. The results at follow-up were rated as excellent in sixty-six elbows, good in nine, fair in eleven, and failed in two. There was an over-all improvement rate of 97.7 per cent, and 85.2 per cent of the patients returned to full activity including rigorous sports.", "contents": "Tennis elbow. The surgical treatment of lateral epicondylitis. Of the 1,213 clinical cases of lateral tennis elbow seen during the time period from December 19, 1971, to October 31, 1977, eighty-eight elbows in eighty-two patients had operative treatment. The lesion that was consistently identified at surgery was immature fibroblastic and vascular infiltration of the origin of the extensor carpi radialis brevis. A specific surgical technique was employed, including exposure of the extensor carpi radialis brevis, excision of the identified lesion, and repair. The results at follow-up were rated as excellent in sixty-six elbows, good in nine, fair in eleven, and failed in two. There was an over-all improvement rate of 97.7 per cent, and 85.2 per cent of the patients returned to full activity including rigorous sports."}
{"id": "PMID:479230", "title": "External pin fixation for unstable Colles' fractures.", "content": "During a five-year period, a double-pin Roger Anderson apparatus, with pins perpendicularly placed in the second and third metacarpals and in the distal part of the radius, was applied in 130 patients with an unstable Colles fracture. Sixty of the 130 were followed for two years. Shortening was limited to a median of two millimeters and dorsal angulation, to a median of 3 degrees. Wrist dorsiflexion averaged 58 degrees, and volar flexion averaged 50 degrees. Pronation and supination had an average loss of 5 degrees when compared with the uninjured side. Repeat reduction was required in only three patients. Patient assessment revealed that 85 per cent of the patients had good results; 12 per cent, fair; and 3 per cent, unsatisfactory. Objective analysis (McBride system) revealed that 90 per cent had good to excellent results; 8 per cent, fair; and 2 per cent, poor. Ninety-two per cent had no pain, 89 per cent had no deformity, and the mean grip strength was twenty kilograms. Sixteen patients had complications; seven of the sixteen had pin loosening, which occurred most frequently late during the course of treatment and without adverse sequelae.", "contents": "External pin fixation for unstable Colles' fractures. During a five-year period, a double-pin Roger Anderson apparatus, with pins perpendicularly placed in the second and third metacarpals and in the distal part of the radius, was applied in 130 patients with an unstable Colles fracture. Sixty of the 130 were followed for two years. Shortening was limited to a median of two millimeters and dorsal angulation, to a median of 3 degrees. Wrist dorsiflexion averaged 58 degrees, and volar flexion averaged 50 degrees. Pronation and supination had an average loss of 5 degrees when compared with the uninjured side. Repeat reduction was required in only three patients. Patient assessment revealed that 85 per cent of the patients had good results; 12 per cent, fair; and 3 per cent, unsatisfactory. Objective analysis (McBride system) revealed that 90 per cent had good to excellent results; 8 per cent, fair; and 2 per cent, poor. Ninety-two per cent had no pain, 89 per cent had no deformity, and the mean grip strength was twenty kilograms. Sixteen patients had complications; seven of the sixteen had pin loosening, which occurred most frequently late during the course of treatment and without adverse sequelae."}
{"id": "PMID:479231", "title": "A simple biplanar method of measuring femoral anteversion and neck-shaft angle.", "content": "A simple biplanar roentgenographic technique for measurement of femoral anteversion and neck-shaft angle is described in which no special equipment is required. An anteroposterior roentgenogram of the pelvis and the femoral neck is made with the hip in neutral rotation. The true lateral roentgenogram is made with the patient's limb in a lateral position with the hip and knee flexed and with the entire lateral aspect of the leg in contact with the top of the table. Projected cervicofemoral angulations are measured on the roentgenograms. The method is described to determine the true angles by dynamically illustrating the projected angles. Graphs have been prepared with which one can readily determine the anteversion and neck-shaft angles once the projected cervicofemoral angulations have been measured.", "contents": "A simple biplanar method of measuring femoral anteversion and neck-shaft angle. A simple biplanar roentgenographic technique for measurement of femoral anteversion and neck-shaft angle is described in which no special equipment is required. An anteroposterior roentgenogram of the pelvis and the femoral neck is made with the hip in neutral rotation. The true lateral roentgenogram is made with the patient's limb in a lateral position with the hip and knee flexed and with the entire lateral aspect of the leg in contact with the top of the table. Projected cervicofemoral angulations are measured on the roentgenograms. The method is described to determine the true angles by dynamically illustrating the projected angles. Graphs have been prepared with which one can readily determine the anteversion and neck-shaft angles once the projected cervicofemoral angulations have been measured."}
{"id": "PMID:479233", "title": "Patellar complications following total knee arthroplasty.", "content": "We studied the complications involving the patella following total knee arthroplasty in eighty-six knees in which thirty-four unconstrained and fifty-two offset hinge prostheses had been implanted. The abnormalities that we studied included: patellar dislocation, five knees; subluxation, eighteen knees; localized wear, three knees; and generalized wear, four knees. In twenty-three patients these complications were associated with patellar malalignment, and occurred predominantly when the offset hinge model of prosthesis was used. The complications were attributed partly to mechanical factors inherent in the prosthetic design and partly to anatomical abnormalities. Failure of the surgeon to compensate adequately for both of those factors at the time of operation was a factor in most of the complications.", "contents": "Patellar complications following total knee arthroplasty. We studied the complications involving the patella following total knee arthroplasty in eighty-six knees in which thirty-four unconstrained and fifty-two offset hinge prostheses had been implanted. The abnormalities that we studied included: patellar dislocation, five knees; subluxation, eighteen knees; localized wear, three knees; and generalized wear, four knees. In twenty-three patients these complications were associated with patellar malalignment, and occurred predominantly when the offset hinge model of prosthesis was used. The complications were attributed partly to mechanical factors inherent in the prosthetic design and partly to anatomical abnormalities. Failure of the surgeon to compensate adequately for both of those factors at the time of operation was a factor in most of the complications."}
{"id": "PMID:479234", "title": "Late reconstruction of the patellar tendon.", "content": "Late reconstruction of undiagnosed ruptures of the patellar tendon by transfer of the gracilis and semitendinosus tendons, supplemented by a heavy-gauge encircling wire to bridge the gap, was successful in four patients. These tendons are stronger than fascial strips which have previously been used for the purpose. The four patients treated by this technique returned to their preinjury functional levels.", "contents": "Late reconstruction of the patellar tendon. Late reconstruction of undiagnosed ruptures of the patellar tendon by transfer of the gracilis and semitendinosus tendons, supplemented by a heavy-gauge encircling wire to bridge the gap, was successful in four patients. These tendons are stronger than fascial strips which have previously been used for the purpose. The four patients treated by this technique returned to their preinjury functional levels."}
{"id": "PMID:479235", "title": "Prosthetic replacement of the knee and a large segment of the femur or tibia.", "content": "Total replacement of the knee and of a long segment of the femur or tibia provided functional recovery of the knee joint in thirteen patients in whom the length of the bone replaced ranged from seventy-five to 150 millimeters. Nine patients had benign bone tumors, three had malignant lesions, and one patient had salvage of a failed total knee arthroplasty, associated with loss of bone structure. After follow-up of two years or more, all patients had a stable extremity, excellent relief of pain, and a useful range of motion, although one patient required revision for loosening.", "contents": "Prosthetic replacement of the knee and a large segment of the femur or tibia. Total replacement of the knee and of a long segment of the femur or tibia provided functional recovery of the knee joint in thirteen patients in whom the length of the bone replaced ranged from seventy-five to 150 millimeters. Nine patients had benign bone tumors, three had malignant lesions, and one patient had salvage of a failed total knee arthroplasty, associated with loss of bone structure. After follow-up of two years or more, all patients had a stable extremity, excellent relief of pain, and a useful range of motion, although one patient required revision for loosening."}
{"id": "PMID:479236", "title": "Joint replacement in non-ambulatory patients.", "content": "We treated eighteen non-ambulatory patients by multiple operations consisting of total replacement of the hip or the knee, or both. Thirteen patients had rheumatoid arthritis, four had degenerative arthritis, and one had had bilateral resection of the femoral head and neck. The patients were followed for an average of forty months. We devised a classification based on the number of joints involved and a rating scale for function. Good to excellent results were achieved in the patients who had two or three joints operated on (with one exception). Fifteen patients became ambulatory and seven could climb stairs. Seven patients were pain-free. The factors responsible for poor results were significant neural problems and loss of motivation. The presence of severe upper-extremity involvement was not an obstacle preventing walking.", "contents": "Joint replacement in non-ambulatory patients. We treated eighteen non-ambulatory patients by multiple operations consisting of total replacement of the hip or the knee, or both. Thirteen patients had rheumatoid arthritis, four had degenerative arthritis, and one had had bilateral resection of the femoral head and neck. The patients were followed for an average of forty months. We devised a classification based on the number of joints involved and a rating scale for function. Good to excellent results were achieved in the patients who had two or three joints operated on (with one exception). Fifteen patients became ambulatory and seven could climb stairs. Seven patients were pain-free. The factors responsible for poor results were significant neural problems and loss of motivation. The presence of severe upper-extremity involvement was not an obstacle preventing walking."}
{"id": "PMID:479237", "title": "Tenosynovial chondromatosis in the hand.", "content": "Eight cases of tenosynovial chondromatosis in the hand were compared with twelve cases collected from the literature and available data on eleven cases showed that in three there were recurrences. No patients had metastatic lesions during a follow-up period averaging 7.8 years. Based on these data, we concluded that a moderately radical en bloc excision is adequate treatment for tenosynovial chondromatosis in the hand.", "contents": "Tenosynovial chondromatosis in the hand. Eight cases of tenosynovial chondromatosis in the hand were compared with twelve cases collected from the literature and available data on eleven cases showed that in three there were recurrences. No patients had metastatic lesions during a follow-up period averaging 7.8 years. Based on these data, we concluded that a moderately radical en bloc excision is adequate treatment for tenosynovial chondromatosis in the hand."}
{"id": "PMID:479238", "title": "A simplified technique to correct hyperextension deformity of the metacarpophalangeal joint of the thumb.", "content": "A flexible hyperextension deformity of the metacarpophalangeal joint of the thumb in eleven patients was repaired by tendon reinforcement of the volar capsule of the joint using the tendon of the extensor pollicis brevis. This tendon, which was regarded as a deforming factor, was left attached at its insertion on the proximal phalanx and was divided at the musculotendinous junction proximally. The mobilized tendon was pulled out distally and then passed proximally and volarly, first superficial to the sheath of the long flexor tendon and then back through a hole in the neck of the metacarpal, and finally in a distal direction to form a criss-cross pattern and be sutured to the tendon of the adductor pollicis. The procedure gave reliable results after an average follow-up of 6.5 years in these eleven patients who had rheumatoid, paralytic, and traumatic lesions.", "contents": "A simplified technique to correct hyperextension deformity of the metacarpophalangeal joint of the thumb. A flexible hyperextension deformity of the metacarpophalangeal joint of the thumb in eleven patients was repaired by tendon reinforcement of the volar capsule of the joint using the tendon of the extensor pollicis brevis. This tendon, which was regarded as a deforming factor, was left attached at its insertion on the proximal phalanx and was divided at the musculotendinous junction proximally. The mobilized tendon was pulled out distally and then passed proximally and volarly, first superficial to the sheath of the long flexor tendon and then back through a hole in the neck of the metacarpal, and finally in a distal direction to form a criss-cross pattern and be sutured to the tendon of the adductor pollicis. The procedure gave reliable results after an average follow-up of 6.5 years in these eleven patients who had rheumatoid, paralytic, and traumatic lesions."}
{"id": "PMID:479239", "title": "Multiple carpometacarpal dislocations. A review of four cases.", "content": "The cases of four patients with carpometacarpal fracture-dislocations of the ulnar four joints are reported, in all of whom there was extensive additional trauma. Open reduction was necessary in three patients, and only one was treated successfully by a closed reduction. He then had persistent but painless subluxation of the metacarpal bases. On end-result evaluation, two of the other three patients had occasional discomfort and diminished grip strength. One patient had a diminished range of motion of the wrist joint at one-year follow-up, and one patient had narrowing and sclerosis of the carpometacarpal joints.", "contents": "Multiple carpometacarpal dislocations. A review of four cases. The cases of four patients with carpometacarpal fracture-dislocations of the ulnar four joints are reported, in all of whom there was extensive additional trauma. Open reduction was necessary in three patients, and only one was treated successfully by a closed reduction. He then had persistent but painless subluxation of the metacarpal bases. On end-result evaluation, two of the other three patients had occasional discomfort and diminished grip strength. One patient had a diminished range of motion of the wrist joint at one-year follow-up, and one patient had narrowing and sclerosis of the carpometacarpal joints."}
{"id": "PMID:479240", "title": "Open reduction for congenital dislocation of the hip using the Ferguson procedure. A review of twenty-six cases.", "content": "Twenty-three patients with twenty-six congenital hip dislocations treated by the Ferguson procedure were reviewed. The study was undertaken to determine if this method of treatment has any effect on the incidence of avascular necrosis of the femoral head and if there are preoperative factors that can assist in predicting the success of treatment. Clinical and roentgenographic criteria were utilized and only dislocations of the hip unassociated with other disorders were included. Avascular necrosis did not occur in these patients during the period studied. It was also found that patients who were more than twelve months old at the time of operation and had a acetabular index of 40 degrees or more had a high incidence of redislocation.", "contents": "Open reduction for congenital dislocation of the hip using the Ferguson procedure. A review of twenty-six cases. Twenty-three patients with twenty-six congenital hip dislocations treated by the Ferguson procedure were reviewed. The study was undertaken to determine if this method of treatment has any effect on the incidence of avascular necrosis of the femoral head and if there are preoperative factors that can assist in predicting the success of treatment. Clinical and roentgenographic criteria were utilized and only dislocations of the hip unassociated with other disorders were included. Avascular necrosis did not occur in these patients during the period studied. It was also found that patients who were more than twelve months old at the time of operation and had a acetabular index of 40 degrees or more had a high incidence of redislocation."}
{"id": "PMID:479241", "title": "Excisional arthroplasty of the elbow.", "content": "Thirty patients had excisional arthroplasty of the elbow, with interpositional Gelfoam and early mobilization. Most of the patients had residual disability from trauma. Most of the patients' results were evaluated four years or more after the operation: 80 per cent were pain-free and 90 per cent were able to return to their previous occupations. One patient had to be reoperated on and therefore was considered to have a poor result.", "contents": "Excisional arthroplasty of the elbow. Thirty patients had excisional arthroplasty of the elbow, with interpositional Gelfoam and early mobilization. Most of the patients had residual disability from trauma. Most of the patients' results were evaluated four years or more after the operation: 80 per cent were pain-free and 90 per cent were able to return to their previous occupations. One patient had to be reoperated on and therefore was considered to have a poor result."}
{"id": "PMID:479242", "title": "Stiff-legged gait in hemiplegia: surgical correction.", "content": "Selective tenotomy of one or two heads of the quadriceps based on electromyographic criteria improved knee flexion in hemiplegic patients who walked with a unilateral stiff-legged gait. The improvement was greatest in eight patients in whom the rectus femoris was released, either with or without release of the vastus intermedius, with activity in pre-swing stance and initial swing confined to those heads of the quadriceps; in these patients knee flexion improved an average of 20 degrees. Knee flexion improved an average of only 8 degrees in five patients in who activity was present in one head of the quadriceps that was not surgically released. No improvement in knee flexion occurred in seven of eight patients in whom activity was present in two or more of the quadriceps heads that were not surgically released.", "contents": "Stiff-legged gait in hemiplegia: surgical correction. Selective tenotomy of one or two heads of the quadriceps based on electromyographic criteria improved knee flexion in hemiplegic patients who walked with a unilateral stiff-legged gait. The improvement was greatest in eight patients in whom the rectus femoris was released, either with or without release of the vastus intermedius, with activity in pre-swing stance and initial swing confined to those heads of the quadriceps; in these patients knee flexion improved an average of 20 degrees. Knee flexion improved an average of only 8 degrees in five patients in who activity was present in one head of the quadriceps that was not surgically released. No improvement in knee flexion occurred in seven of eight patients in whom activity was present in two or more of the quadriceps heads that were not surgically released."}
{"id": "PMID:479243", "title": "Differential bone-scanning in the evaluation of a painful total joint replacement.", "content": "In an attempt to differentiate between loose and infected total joint replacements, seventy-nine patients were studied with both technetium-99 pyrophosphate and gallium-67 citrate bone scans. Seventy of these patients had positive technetium scans. Nineteen had positive gallium scans and all nineteen had positive cultures at the time of operation, while one patient had a false-negative gallium scan. Ten other patients who had negative technetium-99 scans and a painful total joint were followed without operative intervention. Of these, only two required operation after their technetium-99 scans became positive. Based on these studies, gallium-67 citrate scans combined with technetium-99 pyrophosphate scans are believed to be useful in differentiating between loose and infected prostheses. A negative technetium scan should weigh heavily against surgical exploration in an attempt to find the source of pain.", "contents": "Differential bone-scanning in the evaluation of a painful total joint replacement. In an attempt to differentiate between loose and infected total joint replacements, seventy-nine patients were studied with both technetium-99 pyrophosphate and gallium-67 citrate bone scans. Seventy of these patients had positive technetium scans. Nineteen had positive gallium scans and all nineteen had positive cultures at the time of operation, while one patient had a false-negative gallium scan. Ten other patients who had negative technetium-99 scans and a painful total joint were followed without operative intervention. Of these, only two required operation after their technetium-99 scans became positive. Based on these studies, gallium-67 citrate scans combined with technetium-99 pyrophosphate scans are believed to be useful in differentiating between loose and infected prostheses. A negative technetium scan should weigh heavily against surgical exploration in an attempt to find the source of pain."}
{"id": "PMID:479251", "title": "Volkmann's contracture in children: aetiology and prevention.", "content": "A review was conducted of the records of fifty-five children who were admitted to the Hospital for Sick Children in Toronto between 1955 and 1975 with a diagnosis of Volkmann's contracture in fifty-eight limbs. Ten patients had been transferred to this hospital with established ischaemia after Bryant's traction for a fractured femur; all had a very poor outcome. Thirteen other cases of Volkmann's contracture affecting the superficial posterior compartment had been treated with a fixed Thomas' splint and a Bradford frame after fractures of the femoral shaft. Supracondylar fractures of the elbow resulting in Volkmann's contracture frequently had both an arterial injury and a compartment syndrome. Most of the fifty-five children reviewed here had not had early appropriate treatment. For the past twenty-one years the frequency of Volkmann's contracture has not declined in spite of many published reports on the compartment syndrome, and the hazards of supracondylar fractures and of Bryant's traction.", "contents": "Volkmann's contracture in children: aetiology and prevention. A review was conducted of the records of fifty-five children who were admitted to the Hospital for Sick Children in Toronto between 1955 and 1975 with a diagnosis of Volkmann's contracture in fifty-eight limbs. Ten patients had been transferred to this hospital with established ischaemia after Bryant's traction for a fractured femur; all had a very poor outcome. Thirteen other cases of Volkmann's contracture affecting the superficial posterior compartment had been treated with a fixed Thomas' splint and a Bradford frame after fractures of the femoral shaft. Supracondylar fractures of the elbow resulting in Volkmann's contracture frequently had both an arterial injury and a compartment syndrome. Most of the fifty-five children reviewed here had not had early appropriate treatment. For the past twenty-one years the frequency of Volkmann's contracture has not declined in spite of many published reports on the compartment syndrome, and the hazards of supracondylar fractures and of Bryant's traction."}
{"id": "PMID:479252", "title": "The pathology and prevention of Volkmann's ischaemic contracture.", "content": "Ischaemia is a rare complication of injury to a limb but must be excluded in every case. Two distinct types occur: Type I, where a proximal arterial injury gives rise to ischaemia distally; and Type II, where a direct injury gives rise to ischaemia at the site of the injury. Whatever the nature of the insult, an ischaemic contracture only develops as a result of swelling of the soft tissues where these soft tissues are contained in un unyielding osteofascial compartment. This secondary ischaemia can only be relieved by a timely fasciotomy. The diagnosis of ischaemia in an injured limb and the indications to operate on it can usually be made on clinical grounds alone.", "contents": "The pathology and prevention of Volkmann's ischaemic contracture. Ischaemia is a rare complication of injury to a limb but must be excluded in every case. Two distinct types occur: Type I, where a proximal arterial injury gives rise to ischaemia distally; and Type II, where a direct injury gives rise to ischaemia at the site of the injury. Whatever the nature of the insult, an ischaemic contracture only develops as a result of swelling of the soft tissues where these soft tissues are contained in un unyielding osteofascial compartment. This secondary ischaemia can only be relieved by a timely fasciotomy. The diagnosis of ischaemia in an injured limb and the indications to operate on it can usually be made on clinical grounds alone."}
{"id": "PMID:479253", "title": "Factors in the development of the spinal stenosis syndrome.", "content": "The spinal stenosis syndrome is a potential hazard when congenital or developmental narrowing of the bony canal, particularly in its lateral recesses where it can be demonstrated by axial tomography, places the emergent nerve root and its blood supply at risk to further small compressive elements. When lumbar disc degeneration allows rotatory and lateral instability, posterolateral bulging of the annulus fibrosus into the root canal occurs when weight is taken on the ipsilateral lower limb. Symptoms of the spinal stenosis syndrome do not arise until the development of this instability. To relieve the symptoms, the nerve root must be freely mobilised and decompressed by full lateral decompression, with partial or total facetectomy if necessary, by enucleation of the intervertebral disc and by removal of the posterolateral portions of the annuli fibrosi.", "contents": "Factors in the development of the spinal stenosis syndrome. The spinal stenosis syndrome is a potential hazard when congenital or developmental narrowing of the bony canal, particularly in its lateral recesses where it can be demonstrated by axial tomography, places the emergent nerve root and its blood supply at risk to further small compressive elements. When lumbar disc degeneration allows rotatory and lateral instability, posterolateral bulging of the annulus fibrosus into the root canal occurs when weight is taken on the ipsilateral lower limb. Symptoms of the spinal stenosis syndrome do not arise until the development of this instability. To relieve the symptoms, the nerve root must be freely mobilised and decompressed by full lateral decompression, with partial or total facetectomy if necessary, by enucleation of the intervertebral disc and by removal of the posterolateral portions of the annuli fibrosi."}
{"id": "PMID:479254", "title": "Segmental neurophysiological mechanisms in scoliosis.", "content": "Segmental spinal reflexes (stretch reflexes) were studied in patients with scoliosis. The proprioceptive responses to the phasic stretch of the paraspinal muscles were asymmetric in all patients, and were increased on the convex side. The asymmetry was more pronounced when the patients were standing. The observed asymmetry of the reflex responses was taken to indicate asymmetry in the tone and postural activity of the superficial layer of the paraspinal muscles. A reciprocal relationship was found in the segmental reflex organisation between the superficial and deep layers of the paraspinal muscles. The increase in reflex response of the superficial muscles on the convex side can be due to diminished reciprocal inhibition from weak, deep muscles. Thus a segmental neurogenic disorder involving predominantly the deep paraspinal muscles of the convexity of the curve may be the primary lesion responsible for the development of scoliosis.", "contents": "Segmental neurophysiological mechanisms in scoliosis. Segmental spinal reflexes (stretch reflexes) were studied in patients with scoliosis. The proprioceptive responses to the phasic stretch of the paraspinal muscles were asymmetric in all patients, and were increased on the convex side. The asymmetry was more pronounced when the patients were standing. The observed asymmetry of the reflex responses was taken to indicate asymmetry in the tone and postural activity of the superficial layer of the paraspinal muscles. A reciprocal relationship was found in the segmental reflex organisation between the superficial and deep layers of the paraspinal muscles. The increase in reflex response of the superficial muscles on the convex side can be due to diminished reciprocal inhibition from weak, deep muscles. Thus a segmental neurogenic disorder involving predominantly the deep paraspinal muscles of the convexity of the curve may be the primary lesion responsible for the development of scoliosis."}
{"id": "PMID:479255", "title": "Two-stage corrective surgery for congenital deformities of the spine.", "content": "Sixty patients with congenital deformities of the spine were operated upon in the past fifteen years using a two-stage procedure. In the fifty patients with scoliosis half of the deformities were due to hemivertebrae and half to unilateral bars. The average correction of the deformity was 47 per cent. Early neurological signs observed in two patients with a diastematomyelia resolved. Of the ten patients with kyphosis nine had neurological signs of impending paraplegia and one was completely paraplegic before operation; all improved markedly. Posterior spinal fusion alone in the rapidly progressing congenital deformity may not prevent further progression, particularly in those cases iwth unilateral bars. Anterior resection of the vertebral body with later posterior fusion with Harrington instrumentation is safe and effective.", "contents": "Two-stage corrective surgery for congenital deformities of the spine. Sixty patients with congenital deformities of the spine were operated upon in the past fifteen years using a two-stage procedure. In the fifty patients with scoliosis half of the deformities were due to hemivertebrae and half to unilateral bars. The average correction of the deformity was 47 per cent. Early neurological signs observed in two patients with a diastematomyelia resolved. Of the ten patients with kyphosis nine had neurological signs of impending paraplegia and one was completely paraplegic before operation; all improved markedly. Posterior spinal fusion alone in the rapidly progressing congenital deformity may not prevent further progression, particularly in those cases iwth unilateral bars. Anterior resection of the vertebral body with later posterior fusion with Harrington instrumentation is safe and effective."}
{"id": "PMID:479256", "title": "A prospective study of children with untreated Catterall group 1 Perthes' disease.", "content": "Twenty-four hips in twenty children affected by Group 1 Perthes' disease have been reviewed to assess the radiographic result after a minimum follow-up of four years. The children were allocated to Group 1 prospectively after examination of the early radiographs and no specific treatment of the affected hip was provided. The radiographic end results assessed by three methods were good even in those cases in which the additional stress of containment splintage of the contralateral hip was applied.", "contents": "A prospective study of children with untreated Catterall group 1 Perthes' disease. Twenty-four hips in twenty children affected by Group 1 Perthes' disease have been reviewed to assess the radiographic result after a minimum follow-up of four years. The children were allocated to Group 1 prospectively after examination of the early radiographs and no specific treatment of the affected hip was provided. The radiographic end results assessed by three methods were good even in those cases in which the additional stress of containment splintage of the contralateral hip was applied."}
{"id": "PMID:479257", "title": "The African neonatal hip and its immunity from congenital dislocation.", "content": "The hips of twenty full-term African neonates have been examined in detail to determine any anatomical factors which might explain the difference in the incidence of congenital dislocation of the hip in the African and in the Caucasian. Measurements included the degree of anteversion of the femoral neck and the acetabulum and the diameter and depth of the acetabulum. The acetabulum tended to be deeper and to vary within a much narrower range than that reported for Caucasians, lending indirect support to the theoretical role of acetabular dysplasia in the aetiology of congenital dislocation of the hip. Measurements of the anteversion of the acetabulum and femoral neck were similar to those given for Caucasians.", "contents": "The African neonatal hip and its immunity from congenital dislocation. The hips of twenty full-term African neonates have been examined in detail to determine any anatomical factors which might explain the difference in the incidence of congenital dislocation of the hip in the African and in the Caucasian. Measurements included the degree of anteversion of the femoral neck and the acetabulum and the diameter and depth of the acetabulum. The acetabulum tended to be deeper and to vary within a much narrower range than that reported for Caucasians, lending indirect support to the theoretical role of acetabular dysplasia in the aetiology of congenital dislocation of the hip. Measurements of the anteversion of the acetabulum and femoral neck were similar to those given for Caucasians."}
{"id": "PMID:479258", "title": "Failure of sliding nail-plate fixation in subcapital fractures of the femoral neck.", "content": "A retrospective study of 200 cases of subcapital fracture of the neck of the femur treated by sliding nail-plate fixation has been carried out. Failure of fixation within three months occurred in forty-two cases (21 per cent). These failures could, in part, be attributed to a combination of the severity of the fracture and various imperfections in technique. The age of the patient and the angle of the nail-plate had no significant effect on the result.", "contents": "Failure of sliding nail-plate fixation in subcapital fractures of the femoral neck. A retrospective study of 200 cases of subcapital fracture of the neck of the femur treated by sliding nail-plate fixation has been carried out. Failure of fixation within three months occurred in forty-two cases (21 per cent). These failures could, in part, be attributed to a combination of the severity of the fracture and various imperfections in technique. The age of the patient and the angle of the nail-plate had no significant effect on the result."}
{"id": "PMID:479259", "title": "Chip fractures of the os triquetrum: the mechanism of injury.", "content": "Studies derived from analyses of radiographs and dissections of cadaveric wrists have been directed at testing and disproving the commonly held theories that link chip fractures of the os triquetrum with avulsion. The authors found that the mechanism of such injuries involved a chisel action of the ulnar styloid upon the dorsum of the os triquetrum; furthermore, the impact of a fall on the outstretched arm with the hand rigidly held in strong dorsiflexion and ulnar deviation could be forceful enough to fracture the body of the os triquetrum as well. In all cases a striking prolongation of the ulnar styloid, beyond the surface of the ulnar head, was consistently noted.", "contents": "Chip fractures of the os triquetrum: the mechanism of injury. Studies derived from analyses of radiographs and dissections of cadaveric wrists have been directed at testing and disproving the commonly held theories that link chip fractures of the os triquetrum with avulsion. The authors found that the mechanism of such injuries involved a chisel action of the ulnar styloid upon the dorsum of the os triquetrum; furthermore, the impact of a fall on the outstretched arm with the hand rigidly held in strong dorsiflexion and ulnar deviation could be forceful enough to fracture the body of the os triquetrum as well. In all cases a striking prolongation of the ulnar styloid, beyond the surface of the ulnar head, was consistently noted."}
{"id": "PMID:479260", "title": "A comparison of the Freeman-Swanson (ICLH) and Walldius prostheses in total knee replacement.", "content": "Seventy-five Freeman-Swanson (ICLH) Mark I total knee replacements, all performed in one orthopaedic unit between 1972 and 1975, were independently reviewed. The fifty-eight surviving patients, with sixty-eight arthroplasties, have been interviewed and examined and the clinical records of the deceased patients inspected. Sixty arthroplasties (80 per cent) were successful and fifteen failed (20 per cent). There were no disasters. Twenty (33.8 per cent) of the successful arthroplasties were excellent. It is expected that modification of the prosthesis and improved instrumentation will increase this percentage of excellent results and reduce the failure rate.", "contents": "A comparison of the Freeman-Swanson (ICLH) and Walldius prostheses in total knee replacement. Seventy-five Freeman-Swanson (ICLH) Mark I total knee replacements, all performed in one orthopaedic unit between 1972 and 1975, were independently reviewed. The fifty-eight surviving patients, with sixty-eight arthroplasties, have been interviewed and examined and the clinical records of the deceased patients inspected. Sixty arthroplasties (80 per cent) were successful and fifteen failed (20 per cent). There were no disasters. Twenty (33.8 per cent) of the successful arthroplasties were excellent. It is expected that modification of the prosthesis and improved instrumentation will increase this percentage of excellent results and reduce the failure rate."}
{"id": "PMID:479261", "title": "Free vascularised fibular transplant for replacement of the lower radius.", "content": "An operation is described in which a microvascular technique was used to transfer a living fibula, with its vascular pedicle intact, to replace the lower end of the radius after massive resection for giant-cell tumour. Angiography carried out six weeks later showed that the grafted bone was viable. Six months after operation the transplanted fibula showed no osteoporosis or bone resorption and bony union at the junction of host and graft.", "contents": "Free vascularised fibular transplant for replacement of the lower radius. An operation is described in which a microvascular technique was used to transfer a living fibula, with its vascular pedicle intact, to replace the lower end of the radius after massive resection for giant-cell tumour. Angiography carried out six weeks later showed that the grafted bone was viable. Six months after operation the transplanted fibula showed no osteoporosis or bone resorption and bony union at the junction of host and graft."}
{"id": "PMID:479262", "title": "Extra-abdominal desmoid tumours.", "content": "Desmoid tumours are not common but have a distinct resemblance to fibrosarcomata. Their clinical appearance and progress should be recognised since failure to distinguish them from sarcomata could result in extensive and unnecessarily mutilating operations. Three cases of extra-abdominal desmoid tumours are reported. Two of the patients had tumours arising from multicentric foci in the same limb. The disappearance of the tumours in two patients after the menopause, and the variation in the tumours during the menstrual cycle in the third patient, add weight to the theories about endocrine control.", "contents": "Extra-abdominal desmoid tumours. Desmoid tumours are not common but have a distinct resemblance to fibrosarcomata. Their clinical appearance and progress should be recognised since failure to distinguish them from sarcomata could result in extensive and unnecessarily mutilating operations. Three cases of extra-abdominal desmoid tumours are reported. Two of the patients had tumours arising from multicentric foci in the same limb. The disappearance of the tumours in two patients after the menopause, and the variation in the tumours during the menstrual cycle in the third patient, add weight to the theories about endocrine control."}
{"id": "PMID:479263", "title": "[Neoplastic and preneoplastic lesions in rats after oral administration of a single dose of N-nitrosomorpholine (author's transl)].", "content": "The administration of a single oral dose of 320 mg/kg body wt. N-nitrosomorpholine (NNM) to 62 Sprague-dawley rats resulted in neoplastic and preneoplastic changes in different organs, especially in liver and kidney. After a lag period of 4 weeks, nearly all experimental animals developed preneoplastic (clear cell, acidophilic, basophilic, mixed cell) foci of the liver parenchyma. Sporadically, small neoplastic nodules were found in the liver as early as 4 weeks after the beginning of the experiment. After a lag period of 1--2 years, 4 of 13 rats showed multiple neoplastic hepatic nodules and one animal a hepatocellular carcinoma. The bile ductules of some animals responded to the carcinogen by forming mucous cholangiofibroses and cystic cholangiomas. After long lag periods, large cholangiofibromas were found in two experimental animals. One or two years after application of the carcinogen, many animals developed epithelial (clear cell, acidophilic, chromophobic, basophilic, oncocytic) kidney tumors, often cystic. Pathologically changed (clear cell, chromophobic, basophilic, oncocytic) tubules are regarded as precursors of the epithelial tumors. The latered tubules appear for the first time at about half a year after application of the carcinogen. Apart from multiple cysts of the liver and kidneys some pancreatic cysts developed in two animals. In addition, two mesenchymal kidney tumors, one malignant neurinoma, two subcutaneous fibromas, one fibroadenoma, and one squamous cell carcinoma of the skin were observed.", "contents": "[Neoplastic and preneoplastic lesions in rats after oral administration of a single dose of N-nitrosomorpholine (author's transl)]. The administration of a single oral dose of 320 mg/kg body wt. N-nitrosomorpholine (NNM) to 62 Sprague-dawley rats resulted in neoplastic and preneoplastic changes in different organs, especially in liver and kidney. After a lag period of 4 weeks, nearly all experimental animals developed preneoplastic (clear cell, acidophilic, basophilic, mixed cell) foci of the liver parenchyma. Sporadically, small neoplastic nodules were found in the liver as early as 4 weeks after the beginning of the experiment. After a lag period of 1--2 years, 4 of 13 rats showed multiple neoplastic hepatic nodules and one animal a hepatocellular carcinoma. The bile ductules of some animals responded to the carcinogen by forming mucous cholangiofibroses and cystic cholangiomas. After long lag periods, large cholangiofibromas were found in two experimental animals. One or two years after application of the carcinogen, many animals developed epithelial (clear cell, acidophilic, chromophobic, basophilic, oncocytic) kidney tumors, often cystic. Pathologically changed (clear cell, chromophobic, basophilic, oncocytic) tubules are regarded as precursors of the epithelial tumors. The latered tubules appear for the first time at about half a year after application of the carcinogen. Apart from multiple cysts of the liver and kidneys some pancreatic cysts developed in two animals. In addition, two mesenchymal kidney tumors, one malignant neurinoma, two subcutaneous fibromas, one fibroadenoma, and one squamous cell carcinoma of the skin were observed."}
{"id": "PMID:479264", "title": "Tumors and hyperplastic lesions in Syrian hamsters following transplacental and neonatal treatment with cigarette smoke condensate.", "content": "Cigarette smoke condensate (CSC) in olive oil was injected into outbred Syrian hamsters: in adults i.p. on the 10th--14th days of gestation, total dose 1.5--2.5 mg/g b.w.; in 12 to 14-days-old animals s.c., total dose 0.5--1.5 mg/animal. Following 15--25 months of observation benign and malignant neoplasms of various location were found in 2/58 (3.4%) females, treated during pregnancy; in 17/51 (33.3%) of their transplacentally exposed offsprings; in 5/53 (9.4%) of neonatally treated hamsters. In the last two groups females were more affected than males. Most frequently occurred tumors of adrenal glands, pancreas, female sex organs, and liver. No tumors appeared in controls, either untreated or injected with olive oil. In addition, hyperplastic lesions, in particular multiple liver cysts and cholangiomatosis were also observed, mainly in animals exposed transplacentally and as neonates.", "contents": "Tumors and hyperplastic lesions in Syrian hamsters following transplacental and neonatal treatment with cigarette smoke condensate. Cigarette smoke condensate (CSC) in olive oil was injected into outbred Syrian hamsters: in adults i.p. on the 10th--14th days of gestation, total dose 1.5--2.5 mg/g b.w.; in 12 to 14-days-old animals s.c., total dose 0.5--1.5 mg/animal. Following 15--25 months of observation benign and malignant neoplasms of various location were found in 2/58 (3.4%) females, treated during pregnancy; in 17/51 (33.3%) of their transplacentally exposed offsprings; in 5/53 (9.4%) of neonatally treated hamsters. In the last two groups females were more affected than males. Most frequently occurred tumors of adrenal glands, pancreas, female sex organs, and liver. No tumors appeared in controls, either untreated or injected with olive oil. In addition, hyperplastic lesions, in particular multiple liver cysts and cholangiomatosis were also observed, mainly in animals exposed transplacentally and as neonates."}
{"id": "PMID:479265", "title": "Cyclophosphamide-resistant Yoshida ascites tumor cells and their cross resistance to some alkylating agents.", "content": "A cyclophosphamide-resistant subline of a Yoshida ascites tumor was developed by giving increasing doses of the drug after transplantation. The effect of several alkylating agents on the cell proliferation of both the sensitive and resistance cell line was compared establishind dose response curves and D50 values. The developed subline revealed a 260 fold resistance to cyclophosphamide. It was completely cross-resistant to hydroperoxycyclophosphamide, whereas for triaziquone, N-oxide-mustard, and N-methyl-mustard only a partial cross resistance existed. These results give further evidence that cyclophosphamide and hydropeoxycyclophosphamide have the same mechanism of action. Regarding the other alkylating agents the results demonstrate differences concerning either the molecular mode of action or protecting effects. A decreased activation or uptake of substance is probably not the base for resistance.", "contents": "Cyclophosphamide-resistant Yoshida ascites tumor cells and their cross resistance to some alkylating agents. A cyclophosphamide-resistant subline of a Yoshida ascites tumor was developed by giving increasing doses of the drug after transplantation. The effect of several alkylating agents on the cell proliferation of both the sensitive and resistance cell line was compared establishind dose response curves and D50 values. The developed subline revealed a 260 fold resistance to cyclophosphamide. It was completely cross-resistant to hydroperoxycyclophosphamide, whereas for triaziquone, N-oxide-mustard, and N-methyl-mustard only a partial cross resistance existed. These results give further evidence that cyclophosphamide and hydropeoxycyclophosphamide have the same mechanism of action. Regarding the other alkylating agents the results demonstrate differences concerning either the molecular mode of action or protecting effects. A decreased activation or uptake of substance is probably not the base for resistance."}
{"id": "PMID:479266", "title": "The effects of 1-methyl-1-nitrosourea on the survival pattern of cycling and noncycling HeLa cells.", "content": "The biological behavior of HeLa cells exposed to 1-methyl-1-nitrosourea was examined by determining the survival fraction in asynchronous and synchronous cultures. Asynchronous cell population exposed to 1-methyl-1-nitrosourea for 1 h exhibited a shoulder type survival curve, indicating that damage must be accumulated before the lethal effect occurs. A fraction of 25% of cells survives the concentration of 100 micrograms/ml. The duration of treatment with the drug did not have a significant effect on the cell survival. The experiments with synchronized cells showed that MNU exhibits the killing in all phases of the cell age, but the most sensitive are these in S phase. However, they are still six times more resistant at the same concentration than the culture in plateau phase. At the concentration of 100 micrograms/ml nondividing plateau cells are about 35 times more sensitive than exponentially growing cells. We can conclude that MNU acts as the most acting killing agents on the cells which are in nondividing plateau phase.", "contents": "The effects of 1-methyl-1-nitrosourea on the survival pattern of cycling and noncycling HeLa cells. The biological behavior of HeLa cells exposed to 1-methyl-1-nitrosourea was examined by determining the survival fraction in asynchronous and synchronous cultures. Asynchronous cell population exposed to 1-methyl-1-nitrosourea for 1 h exhibited a shoulder type survival curve, indicating that damage must be accumulated before the lethal effect occurs. A fraction of 25% of cells survives the concentration of 100 micrograms/ml. The duration of treatment with the drug did not have a significant effect on the cell survival. The experiments with synchronized cells showed that MNU exhibits the killing in all phases of the cell age, but the most sensitive are these in S phase. However, they are still six times more resistant at the same concentration than the culture in plateau phase. At the concentration of 100 micrograms/ml nondividing plateau cells are about 35 times more sensitive than exponentially growing cells. We can conclude that MNU acts as the most acting killing agents on the cells which are in nondividing plateau phase."}
{"id": "PMID:479267", "title": "Excretion patterns of alkylating metabolites in urine following cyclophosphamide treatment of tumor patients: influence of application route, dosage, liver and kidney function.", "content": "The excretion patterns of cyclophosphamide (CP) in urine were studied in 54 tumor patients aged between 21 and 61 years, using the nitrobenzyl-pyridine (NBP) reaction, with regard to the route of application (i.v., i.m. or oral), the CP dose and the functional state of the liver and kidney. The studies were carried out in nephrectomized patients and patients with liver affections caused by the basic disease, in particular with malignant lymphomas and mammary carcinomas. The following results were obtained: 1. There exists a direct relationship between the dose of CP applied and the quantitative excretion of alkylating metabolites in urine. According to these studies in which the patients received up to 2.8 g CP/m2 body surface, the upper CP dose was limited by the generally toxic side effects rather than by the metabolization rate. 2. At comparable CP doses the route of application (i.v., i.m. or oral) has no appreciable influence on the excreted NBP activity. 3. Disorders of the liver function without signs of icterus are not a contraindication to CP treatment. 4. The functional failure of one kidney has no statistically significant influence on the excretion of alkylating metabolites in urine.", "contents": "Excretion patterns of alkylating metabolites in urine following cyclophosphamide treatment of tumor patients: influence of application route, dosage, liver and kidney function. The excretion patterns of cyclophosphamide (CP) in urine were studied in 54 tumor patients aged between 21 and 61 years, using the nitrobenzyl-pyridine (NBP) reaction, with regard to the route of application (i.v., i.m. or oral), the CP dose and the functional state of the liver and kidney. The studies were carried out in nephrectomized patients and patients with liver affections caused by the basic disease, in particular with malignant lymphomas and mammary carcinomas. The following results were obtained: 1. There exists a direct relationship between the dose of CP applied and the quantitative excretion of alkylating metabolites in urine. According to these studies in which the patients received up to 2.8 g CP/m2 body surface, the upper CP dose was limited by the generally toxic side effects rather than by the metabolization rate. 2. At comparable CP doses the route of application (i.v., i.m. or oral) has no appreciable influence on the excreted NBP activity. 3. Disorders of the liver function without signs of icterus are not a contraindication to CP treatment. 4. The functional failure of one kidney has no statistically significant influence on the excretion of alkylating metabolites in urine."}
{"id": "PMID:479268", "title": "Endodermal germ cell carcinoma (endodermal sinus tumor) of the vagina in infant girls.", "content": "An endodermal sinus tumor (endodermal germ cell carcinoma) was diagnosed in a 1-year-old girl in the vagina after hemorrhage; the tumor was completely removed by radical abdominal surgery. Postoperative polychemotherapy was performed for two years with Actinomycin D, Adriamycin, Vincristin, and Cyclophosphamide. The infant is now tumor-free for 26 months, showing almost normal somatic and psychic development. The characteristic histological patterns and clinical course of this strongly malignant tumor are demonstrated, based on 25 published case reports of endodermal sinus tumors in the vagina of little girls (aged 5-26 months). This neoplasm in early infancy has to be separated from the clear-cell adenocarcinoma of the vagina which occurs after puberty in adolescent girls and young women, and is induced by stilbestrol therapy to the mother during early pregnancy.", "contents": "Endodermal germ cell carcinoma (endodermal sinus tumor) of the vagina in infant girls. An endodermal sinus tumor (endodermal germ cell carcinoma) was diagnosed in a 1-year-old girl in the vagina after hemorrhage; the tumor was completely removed by radical abdominal surgery. Postoperative polychemotherapy was performed for two years with Actinomycin D, Adriamycin, Vincristin, and Cyclophosphamide. The infant is now tumor-free for 26 months, showing almost normal somatic and psychic development. The characteristic histological patterns and clinical course of this strongly malignant tumor are demonstrated, based on 25 published case reports of endodermal sinus tumors in the vagina of little girls (aged 5-26 months). This neoplasm in early infancy has to be separated from the clear-cell adenocarcinoma of the vagina which occurs after puberty in adolescent girls and young women, and is induced by stilbestrol therapy to the mother during early pregnancy."}
{"id": "PMID:479269", "title": "Hormone sensitivity of gynecological tumor cells in tissue culture.", "content": "Proliferating tumor cells obtained from ovarian, mammary, and endometrial tumors in tissue culture were tested for the influence of proteohormones and steroid hormones on cellular DNA synthesis and cell growth. The gonadotropic hormones stimulated DNA synthesis of ovarian tumor cells by single administration, or in combination with cortisol, up to the 11-fold of the comparable controls. The hormone sensitivity of the cell lines was variable, resulting in individual reaction patterns. There was no correlation to the histological diagnosis of the primary tumors with respect to the grade of differentiation. The results suggest that ovarian tumor cells in tissue culture can maintain sensitivity to organotropic hormones. Compared to the ovarian carcinoma lines, mammary or endometrial tumor cells did not respond to a similar extent. Progesterone decreased DNA synthesis of endometrial carcinoma cells.", "contents": "Hormone sensitivity of gynecological tumor cells in tissue culture. Proliferating tumor cells obtained from ovarian, mammary, and endometrial tumors in tissue culture were tested for the influence of proteohormones and steroid hormones on cellular DNA synthesis and cell growth. The gonadotropic hormones stimulated DNA synthesis of ovarian tumor cells by single administration, or in combination with cortisol, up to the 11-fold of the comparable controls. The hormone sensitivity of the cell lines was variable, resulting in individual reaction patterns. There was no correlation to the histological diagnosis of the primary tumors with respect to the grade of differentiation. The results suggest that ovarian tumor cells in tissue culture can maintain sensitivity to organotropic hormones. Compared to the ovarian carcinoma lines, mammary or endometrial tumor cells did not respond to a similar extent. Progesterone decreased DNA synthesis of endometrial carcinoma cells."}
{"id": "PMID:479270", "title": "[Malignant interstitial cell tumor of testis with a marker enzyme (author's transl)].", "content": "Metastatic interstitial cell tumor of the testis is one of the rarest human neoplasms. This is the nineteenth case to be reported. While most of these tumors are combined with hormonal dysfunction, the present tumor, apart from its uncommon hormonal profile, is remarkable because of its capacity of producing and secreting a marker enzyme, alkaline phosphatase. No response was seen after cytostatic therapy with new antineoplastic agents, such as a combination of adriamycin and cis-diamminedichloride-platinum (II), and ifosfamide. Considering the lack of radiosensitivity, surgery is the primary modality of treatment.", "contents": "[Malignant interstitial cell tumor of testis with a marker enzyme (author's transl)]. Metastatic interstitial cell tumor of the testis is one of the rarest human neoplasms. This is the nineteenth case to be reported. While most of these tumors are combined with hormonal dysfunction, the present tumor, apart from its uncommon hormonal profile, is remarkable because of its capacity of producing and secreting a marker enzyme, alkaline phosphatase. No response was seen after cytostatic therapy with new antineoplastic agents, such as a combination of adriamycin and cis-diamminedichloride-platinum (II), and ifosfamide. Considering the lack of radiosensitivity, surgery is the primary modality of treatment."}
{"id": "PMID:479271", "title": "Vasospasm in the lower extremities during and following arterial reconstruction.", "content": "Foot temperatures of 36 patients were recorded continuously in the operating room and in the surgical intensive care unit (SICU) for 1-3 days. Operations included aortofemoral endarterectomy, arterioplasty, and bypass grafting. Regardless of severity of the operation, all had cold extremities on entering the SICU. The subsequent warming of the big toes, which started after 4-12 hours in the SICU, if at all, was classified according to a scheme of 6 deviations from a basic trend. This latter was a bilateral, continuous increase in 1-2 hours to 34 degrees C or higher where it remained with minor oscillations. Unilateral deviations were either diminished warming or no warming on one side. Bilateral deviations included stepwise increments to 34 degrees C, diminished increments, and no warming. Neurogenic vasospasm appeared to be the principal factor diminishing blood flow, with circulating vasoconstrictors and organic blocks as additional less important factors. Subsequent amputations of nine limbs were partially correlated with the categories of digital warming.", "contents": "Vasospasm in the lower extremities during and following arterial reconstruction. Foot temperatures of 36 patients were recorded continuously in the operating room and in the surgical intensive care unit (SICU) for 1-3 days. Operations included aortofemoral endarterectomy, arterioplasty, and bypass grafting. Regardless of severity of the operation, all had cold extremities on entering the SICU. The subsequent warming of the big toes, which started after 4-12 hours in the SICU, if at all, was classified according to a scheme of 6 deviations from a basic trend. This latter was a bilateral, continuous increase in 1-2 hours to 34 degrees C or higher where it remained with minor oscillations. Unilateral deviations were either diminished warming or no warming on one side. Bilateral deviations included stepwise increments to 34 degrees C, diminished increments, and no warming. Neurogenic vasospasm appeared to be the principal factor diminishing blood flow, with circulating vasoconstrictors and organic blocks as additional less important factors. Subsequent amputations of nine limbs were partially correlated with the categories of digital warming."}
{"id": "PMID:479273", "title": "Arterial and venous blood viscosity in ischemic lower limbs in patients affected by peripheral obliterative arterial disease.", "content": "Blood, plasma and serum viscosity, packed red cell volume, and plasma fibrinogen concentration was measured in a group of 13 subjects, suffering from peripheral obliterative arterial disease of the lower limbs (stage III or IV), awaiting surgery, and in 9 control subjects, none of whom showed signs of circulatory disease of the lower limbs either by clinical examination or by instrumental measurements. The blood samples were taken from the: (a) antecubital vein; (b) femoral vein; (c) femoral artery. In the patients venous blood viscosity was significantly higher than arterial blood viscosity. No significant differences have been found between the femoral and the antecubital vein, but in the femoral vein blood viscosity was slightly higher. Packed red cell volume and plasma viscosity were slightly but significantly higher in venous blood, while no difference was seen in serum viscosity and in plasma fibrinogen concentration. In the control subjects blood viscosity values were decidedly lower than in patients and no statistically significant differences were to be found between artery and vein, even if viscosity values were lower in artery. These results support the hypothesis that an interrelation exists between hyperivscosity syndrome and vascular ischaemia-inducing diseases.", "contents": "Arterial and venous blood viscosity in ischemic lower limbs in patients affected by peripheral obliterative arterial disease. Blood, plasma and serum viscosity, packed red cell volume, and plasma fibrinogen concentration was measured in a group of 13 subjects, suffering from peripheral obliterative arterial disease of the lower limbs (stage III or IV), awaiting surgery, and in 9 control subjects, none of whom showed signs of circulatory disease of the lower limbs either by clinical examination or by instrumental measurements. The blood samples were taken from the: (a) antecubital vein; (b) femoral vein; (c) femoral artery. In the patients venous blood viscosity was significantly higher than arterial blood viscosity. No significant differences have been found between the femoral and the antecubital vein, but in the femoral vein blood viscosity was slightly higher. Packed red cell volume and plasma viscosity were slightly but significantly higher in venous blood, while no difference was seen in serum viscosity and in plasma fibrinogen concentration. In the control subjects blood viscosity values were decidedly lower than in patients and no statistically significant differences were to be found between artery and vein, even if viscosity values were lower in artery. These results support the hypothesis that an interrelation exists between hyperivscosity syndrome and vascular ischaemia-inducing diseases."}
{"id": "PMID:479275", "title": "Vocal cord paralysis following carotid endarterectomy.", "content": "From 1964 to 1977, over 400 carotid endarterectomies were performed at the Rochester General Hospital. Two patients from this group experienced hoarseness secondary to vocal cord paralysis on the operated side, believed due to trauma to the vagus nerve. Although uncommon, it is important to stress the method of avoiding this complication.", "contents": "Vocal cord paralysis following carotid endarterectomy. From 1964 to 1977, over 400 carotid endarterectomies were performed at the Rochester General Hospital. Two patients from this group experienced hoarseness secondary to vocal cord paralysis on the operated side, believed due to trauma to the vagus nerve. Although uncommon, it is important to stress the method of avoiding this complication."}
{"id": "PMID:479276", "title": "Importance of bidirectional arterial flow in dialysis fistula construction.", "content": "The normal direction of blood flow in end of cephalic vein to side of radial artery arteriovenous dialysis fistulas in the wrist was antegrade in the proximal arterial limb and retrograde through the distal arterial limb. There were no instances of arterial insufficiency in the hand due to a steal phenomenon. These findings support the surgical construction of end of vein to side of artery fistulas.", "contents": "Importance of bidirectional arterial flow in dialysis fistula construction. The normal direction of blood flow in end of cephalic vein to side of radial artery arteriovenous dialysis fistulas in the wrist was antegrade in the proximal arterial limb and retrograde through the distal arterial limb. There were no instances of arterial insufficiency in the hand due to a steal phenomenon. These findings support the surgical construction of end of vein to side of artery fistulas."}
{"id": "PMID:479277", "title": "Long-term results (from 5 to 7 years) with the Hancock S-G-P bioprosthesis.", "content": "This communication is concerned with the long-term results of the first 113 patients who underwent single or multiple heart valve replacement with the Hancock-SGP bioprosthesis at the Department of Cardiovascular Surgery, University of Padova Medical School, in a two years period (March 1970-March 1972). Hospital mortality was 18.5%; of the 92 survivors, whose follow-up is now 5 to more than 7 years, the authors report the clinical reevaluation, the complications and the causes of late death. Hemodynamic reevaluation has been performed so far in 26 patients and the prostheses have shown to function properly both in the mitral and the aortic position. The data available to date indicate that the glutaraldehyde preserved porcine valve is durable and its use is warranted in any valvular position.", "contents": "Long-term results (from 5 to 7 years) with the Hancock S-G-P bioprosthesis. This communication is concerned with the long-term results of the first 113 patients who underwent single or multiple heart valve replacement with the Hancock-SGP bioprosthesis at the Department of Cardiovascular Surgery, University of Padova Medical School, in a two years period (March 1970-March 1972). Hospital mortality was 18.5%; of the 92 survivors, whose follow-up is now 5 to more than 7 years, the authors report the clinical reevaluation, the complications and the causes of late death. Hemodynamic reevaluation has been performed so far in 26 patients and the prostheses have shown to function properly both in the mitral and the aortic position. The data available to date indicate that the glutaraldehyde preserved porcine valve is durable and its use is warranted in any valvular position."}
{"id": "PMID:479278", "title": "Correction of tetralogy of Fallot after Waterston shunt.", "content": "The authors have reviewed their experience concerning twenty-seven patients who underwent intracardiac repair of Fallot's Tetralogy after a previous Waterston shunt. They are divided into 4 groups according to the classification of Shinebourne, Anderson and Bowyer. The risk factors are analyzed in group 1 patients in whom primary total correction is contra-indicated and in whom the mortality at repair is high in presence of kinking of right pulmonary artery. The type of surgery is still debatable. For the symptomatic patients of the other groups, primary total correction at any age seems, at this time, to be the treatment of choice. The contra-indications are an anterior descending coronary artery arising from the right coronary artery or associated complex anomalies which would make total correction difficult.", "contents": "Correction of tetralogy of Fallot after Waterston shunt. The authors have reviewed their experience concerning twenty-seven patients who underwent intracardiac repair of Fallot's Tetralogy after a previous Waterston shunt. They are divided into 4 groups according to the classification of Shinebourne, Anderson and Bowyer. The risk factors are analyzed in group 1 patients in whom primary total correction is contra-indicated and in whom the mortality at repair is high in presence of kinking of right pulmonary artery. The type of surgery is still debatable. For the symptomatic patients of the other groups, primary total correction at any age seems, at this time, to be the treatment of choice. The contra-indications are an anterior descending coronary artery arising from the right coronary artery or associated complex anomalies which would make total correction difficult."}
{"id": "PMID:479283", "title": "On the mechanism of 5-bromodeoxyuridine induction of prolactin synthesis in rat pituitary tumor cells.", "content": "GH12C1, a clonal strain of rat pituitary tumor cells in culture (GH cells), does not produce detectable amounts of prolactin. 5-Bromodeoxyuridine (BrdUrd), the thymidine analogue, at sublethal concentrations (3-5 microgram/ml) induces prolactin synthesis in these cells. BrdUrd also induces prolactin synthesis in F1BGH12C1 cells, a BrdUrd resistant (BrdUrdr) substrain isolated from GH12C1 cells. The F1BGH12C1 strain is not drug dependent, but its resistance to BrdUrd is a stable phenotype. The significant features of the induction of prolactin synthesis in the BrdUrdr strain are the increased net synthesis of prolactin and the shortening of the lag period of prolactin induction. As BrdUrd concentration in the growth medium is increased, the rise in prolactin synthesis parallels the increased incorporation of BrdUrd into DNA. Prolactin synthesis is first detected when BrdUrd replaces 20-25% of the thymidine in DNA. BrdUrd can replace up to 75-80% of the thymidine within 2 d of treatment. Partial starvation of these cells under specified growth conditions does not affect the general growth pattern of the cells, general protein synthesis, and thymidine uptake, but does affect DNA synthesis. When cells are cultured under conditions in which DNA synthesis is preferentially inhibited, BrdUrd does not induce prolactin synthesis, suggestive of a DNA-mediated mechanism of action for the drug.", "contents": "On the mechanism of 5-bromodeoxyuridine induction of prolactin synthesis in rat pituitary tumor cells. GH12C1, a clonal strain of rat pituitary tumor cells in culture (GH cells), does not produce detectable amounts of prolactin. 5-Bromodeoxyuridine (BrdUrd), the thymidine analogue, at sublethal concentrations (3-5 microgram/ml) induces prolactin synthesis in these cells. BrdUrd also induces prolactin synthesis in F1BGH12C1 cells, a BrdUrd resistant (BrdUrdr) substrain isolated from GH12C1 cells. The F1BGH12C1 strain is not drug dependent, but its resistance to BrdUrd is a stable phenotype. The significant features of the induction of prolactin synthesis in the BrdUrdr strain are the increased net synthesis of prolactin and the shortening of the lag period of prolactin induction. As BrdUrd concentration in the growth medium is increased, the rise in prolactin synthesis parallels the increased incorporation of BrdUrd into DNA. Prolactin synthesis is first detected when BrdUrd replaces 20-25% of the thymidine in DNA. BrdUrd can replace up to 75-80% of the thymidine within 2 d of treatment. Partial starvation of these cells under specified growth conditions does not affect the general growth pattern of the cells, general protein synthesis, and thymidine uptake, but does affect DNA synthesis. When cells are cultured under conditions in which DNA synthesis is preferentially inhibited, BrdUrd does not induce prolactin synthesis, suggestive of a DNA-mediated mechanism of action for the drug."}
{"id": "PMID:479279", "title": "Double outlet left ventricle: how should we repair it?", "content": "Two patients with D.O.L.V., V.S.D. and P.S. have been successfully corrected surgically. They represent the seventh and the eighth successful repairs of D.O.L.V. reported so far in the literature. Surgical correction was accomplished by an \"extraventricular\" type of repair, closing the V.S.D., interrupting the connection between left ventricle and pulmonary artery and creating a conduit between right ventricle and pulmonary artery. The conduit used was a fresh aortic homograft. An \"intraventricular\" type of repair of this rare congenital malformation has been also reported, but the authors believe that it is not applicable to all cases of D.O.L.V. and it is possibly more likely to incur complications, while the \"extraventricular\" one doesn't seem to have any contraindication. Preoperative diagnosis can be only achieved with careful angiographic study, including left ventriculography.", "contents": "Double outlet left ventricle: how should we repair it? Two patients with D.O.L.V., V.S.D. and P.S. have been successfully corrected surgically. They represent the seventh and the eighth successful repairs of D.O.L.V. reported so far in the literature. Surgical correction was accomplished by an \"extraventricular\" type of repair, closing the V.S.D., interrupting the connection between left ventricle and pulmonary artery and creating a conduit between right ventricle and pulmonary artery. The conduit used was a fresh aortic homograft. An \"intraventricular\" type of repair of this rare congenital malformation has been also reported, but the authors believe that it is not applicable to all cases of D.O.L.V. and it is possibly more likely to incur complications, while the \"extraventricular\" one doesn't seem to have any contraindication. Preoperative diagnosis can be only achieved with careful angiographic study, including left ventriculography."}
{"id": "PMID:479284", "title": "Hormonal modulation of ovarian interstitial cells with particular reference to gap junctions.", "content": "Thin-section and freeze-fracture studies on the rat ovarian interstitial cells revealed reductions in the size and the number of gap junctions after pituitary ablation. Small gap junctions, however, persist as long as 90 days after hypophysectomy even though regressive cytoplasmic changes are completed 75 d earlier. Administration of exogenous human chorionic gonadotrophin (HCG) results in the restoration of the normal interstitial cell morphology which is accompanied by amplification of junctional membrane. Within 24 h of hormone application, gap junction growth is characterized by the appearance of formation plaques. These studies suggest that the effect of hormone on interstitial cell gap junctions is to modulate the junctional surface area.", "contents": "Hormonal modulation of ovarian interstitial cells with particular reference to gap junctions. Thin-section and freeze-fracture studies on the rat ovarian interstitial cells revealed reductions in the size and the number of gap junctions after pituitary ablation. Small gap junctions, however, persist as long as 90 days after hypophysectomy even though regressive cytoplasmic changes are completed 75 d earlier. Administration of exogenous human chorionic gonadotrophin (HCG) results in the restoration of the normal interstitial cell morphology which is accompanied by amplification of junctional membrane. Within 24 h of hormone application, gap junction growth is characterized by the appearance of formation plaques. These studies suggest that the effect of hormone on interstitial cell gap junctions is to modulate the junctional surface area."}
{"id": "PMID:479280", "title": "Clinical comparison between membrane and bubble oxygenators in cardiopulmonary bypass.", "content": "The clinical course of two similar patient groups was compared in whom, during cardiopulmonary bypass, a membrane or bubble oxygenator was employed. According to our results there is no significant functional difference between the two types of oxygenators as long as the perfusion time does not exceed 90 minutes. Beyond this time limit, the membrane oxygenator has distinct advantages, particularly with regard to hemolysis. We presently prefer the bubble oxygenator. The use of a membrane oxygenator is restricted to complex open heart procedures with suspected technical problems.", "contents": "Clinical comparison between membrane and bubble oxygenators in cardiopulmonary bypass. The clinical course of two similar patient groups was compared in whom, during cardiopulmonary bypass, a membrane or bubble oxygenator was employed. According to our results there is no significant functional difference between the two types of oxygenators as long as the perfusion time does not exceed 90 minutes. Beyond this time limit, the membrane oxygenator has distinct advantages, particularly with regard to hemolysis. We presently prefer the bubble oxygenator. The use of a membrane oxygenator is restricted to complex open heart procedures with suspected technical problems."}
{"id": "PMID:479285", "title": "Myosin synthesis in embryonic chicken fibroblasts.", "content": "The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min.", "contents": "Myosin synthesis in embryonic chicken fibroblasts. The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min."}
{"id": "PMID:479281", "title": "Early thoracotomy for stab wounds of the heart.", "content": "Fifteen consecutive patients with stab wounds of the heart are reviewed. The left ventricle was stabbed in six patients and right ventricle in four. Sinus tachycardia was present in 54% and hypotension in 67% of cases. Central venous pressure recorded in ten patients was elevated in all but one. Generalized ST segment elevation was the rule among the available preoperative recordings. One patient had right bundle branch block. Thirteen of the fifteen patients had a thoracotomy and two had pericardiocentesis only. Sixty nine percent of the thoracotomies were performed within two hours of the injury. There was only one death of a patient who arrived with no blood pressure or heart sounds. An average follow-up of 20 months revealed long-term sequela of an asymptomatic ventricular aneurysm in one patient. Our unusually gratifying results support the approach of emergency thoracotomy in patients with penetrating cardiac trauma.", "contents": "Early thoracotomy for stab wounds of the heart. Fifteen consecutive patients with stab wounds of the heart are reviewed. The left ventricle was stabbed in six patients and right ventricle in four. Sinus tachycardia was present in 54% and hypotension in 67% of cases. Central venous pressure recorded in ten patients was elevated in all but one. Generalized ST segment elevation was the rule among the available preoperative recordings. One patient had right bundle branch block. Thirteen of the fifteen patients had a thoracotomy and two had pericardiocentesis only. Sixty nine percent of the thoracotomies were performed within two hours of the injury. There was only one death of a patient who arrived with no blood pressure or heart sounds. An average follow-up of 20 months revealed long-term sequela of an asymptomatic ventricular aneurysm in one patient. Our unusually gratifying results support the approach of emergency thoracotomy in patients with penetrating cardiac trauma."}
{"id": "PMID:479286", "title": "Generation of asymmetry during development. Segregation of type-specific proteins in Caulobacter.", "content": "An essential event in developmental processes is the introduction of asymmetry into an otherwise undifferentiated cell population. Cell division in Caulobacter is asymmetric; the progeny cells are structurally different and follow different sequences of development, thus providing a useful model system for the study of differentiation. Because the progeny cells are different from one another, there must be a segregation of morphogenetic and informational components at some time in the cell cycle. We have examined the pattern of specific protein segregation between Caulobacter stalked and swarmer daughter cells, with the rationale that such a progeny analysis would identify both structurally and developmentally important proteins. To complement the study, we have also examined the pattern of protein synthesis during synchronous growth and in various cellular fractions. We show here, for the first time, that the association of proteins with a specific cell type may result not only from their periodicity of synthesis, but also from their pattern of distribution at the time of cell division. Several membrane-associated and soluble proteins are segregated asymmetrically between progeny stalked and swarmer cells. The data further show that a subclass of soluble proteins becomes associated with the membrane of the progeny stalked cells. Therefore, although the principal differentiated cell types possess different synthetic capabilities and characteristic proteins, the asymmetry between progeny stalked and swarmer cells is generated primarily by the preferential association of specific soluble proteins with the membrane of only one daughter cell. The majority of the proteins which exhibit this segregation behavior are synthesized during the entire cell cycle and exhibit relatively long, functional messenger RNA half-lives.", "contents": "Generation of asymmetry during development. Segregation of type-specific proteins in Caulobacter. An essential event in developmental processes is the introduction of asymmetry into an otherwise undifferentiated cell population. Cell division in Caulobacter is asymmetric; the progeny cells are structurally different and follow different sequences of development, thus providing a useful model system for the study of differentiation. Because the progeny cells are different from one another, there must be a segregation of morphogenetic and informational components at some time in the cell cycle. We have examined the pattern of specific protein segregation between Caulobacter stalked and swarmer daughter cells, with the rationale that such a progeny analysis would identify both structurally and developmentally important proteins. To complement the study, we have also examined the pattern of protein synthesis during synchronous growth and in various cellular fractions. We show here, for the first time, that the association of proteins with a specific cell type may result not only from their periodicity of synthesis, but also from their pattern of distribution at the time of cell division. Several membrane-associated and soluble proteins are segregated asymmetrically between progeny stalked and swarmer cells. The data further show that a subclass of soluble proteins becomes associated with the membrane of the progeny stalked cells. Therefore, although the principal differentiated cell types possess different synthetic capabilities and characteristic proteins, the asymmetry between progeny stalked and swarmer cells is generated primarily by the preferential association of specific soluble proteins with the membrane of only one daughter cell. The majority of the proteins which exhibit this segregation behavior are synthesized during the entire cell cycle and exhibit relatively long, functional messenger RNA half-lives."}
{"id": "PMID:479287", "title": "Requirements for the insertion of the Sindbis envelope glycoproteins into the endoplasmic reticulum membrane.", "content": "Previous work has shown that the Sindbis structural proteins, core, the internal protein, and PE2 and E1, the integral membrane glycoproteins are synthesized as a polyprotein from a 26S mRNA; core PE2 and E1 are derived by proteolytic cleavage of a nascent chain. Newly synthesized core protein remains on the cytoplasmic side of the endoplasmic reticulum while newly synthesized PE2 and E1 are inserted into the lipid bilayer, presumably via their amino-termini. PE2 and E1 are glycosylated as nascent chains. Here, we examine a temperature-sensitive mutant of Sindbis virus which fails to cleave the structural proteins, resulting in the production of a polyprotein of 130,000 mol wt in which the amino-termini of PE2 and E1 are internal to the protein. Although the envelope sequences are present in this protein, it is not inserted into the endoplasmic reticulum bilayer, but remains on the cytoplasmic side as does the core protein in cells infected with wild-type Sindbis virus. We have also examined the fate of PE2 and E1 in cells treated with tunicamycin, an inhibitor of glycosylation. Unglycosylated PE2 and E1 are inserted normally into the lipid bilayer as are the glycosylated proteins. These results are consistent with the notion that a specific amino-terminal sequence is required for the proper insertion of membrane proteins into the endoplasmic reticulum bilayer, but that glycosylation is not required for this insertion.", "contents": "Requirements for the insertion of the Sindbis envelope glycoproteins into the endoplasmic reticulum membrane. Previous work has shown that the Sindbis structural proteins, core, the internal protein, and PE2 and E1, the integral membrane glycoproteins are synthesized as a polyprotein from a 26S mRNA; core PE2 and E1 are derived by proteolytic cleavage of a nascent chain. Newly synthesized core protein remains on the cytoplasmic side of the endoplasmic reticulum while newly synthesized PE2 and E1 are inserted into the lipid bilayer, presumably via their amino-termini. PE2 and E1 are glycosylated as nascent chains. Here, we examine a temperature-sensitive mutant of Sindbis virus which fails to cleave the structural proteins, resulting in the production of a polyprotein of 130,000 mol wt in which the amino-termini of PE2 and E1 are internal to the protein. Although the envelope sequences are present in this protein, it is not inserted into the endoplasmic reticulum bilayer, but remains on the cytoplasmic side as does the core protein in cells infected with wild-type Sindbis virus. We have also examined the fate of PE2 and E1 in cells treated with tunicamycin, an inhibitor of glycosylation. Unglycosylated PE2 and E1 are inserted normally into the lipid bilayer as are the glycosylated proteins. These results are consistent with the notion that a specific amino-terminal sequence is required for the proper insertion of membrane proteins into the endoplasmic reticulum bilayer, but that glycosylation is not required for this insertion."}
{"id": "PMID:479288", "title": "Local anesthetic-induced inhibition of collagen secretion in cultured cells under conditions where microtubules are not depolymerized by these agents.", "content": "Tertiary amine local anesthetics previously have been shown to influence some microtubule-dependent cellular functions. Since several cell secretion processes, including secretion of collagen, have been shown to be inhibited by microtubule-disrupting drugs such as colchicine, we determined whether local anesthetics affect collagen secretion. Six local anesthetics inhibited collagen and non-collagen protein secretion (up to 98%) into the extracellular medium of 3T3 cells and human fibroblasts, an effect apparently independent of influences on proline transport and total protein synthesis. A combination of colchicine and cytochalasin B did not duplicate the effects of local anesthetics. The effects of subsaturating concentrations of colchicine and procaine on secretion were additive, suggesting that both drugs act on the secretory pathway at the level of microtubules, but other effects of the two types of drugs were strikingly different. In comparing the mechanisms of action of colchicine and local anesthetics, it was seen that, in contrast to colchicine, radioactive procaine and lidocaine were slowly transported into 3T3 cells, did not bind to the tubulin-containing TCA-insoluble fraction, and did not bind to purified tubulin in vitro. The fraction of cellular tubulin present as microtubules (47% in normal cells) was determined by measuring tubulin in stabilized, sedimentable microtubules compared to total tubulin, using a [3H]colchicine binding assay. Pretreatment of cells in the cold or with colchicine led to depolymerization of microtubules, but pretreatment with five local anesthetics tested did not. Therefore, in contrast to colchicine, local anesthetics in concentrations that inhibit secretion do not directly interact with or depolymerize microtubules. These drugs, however, do affect a microtubule-dependent process and may do so by detaching the microtubular system from the cell membrane.", "contents": "Local anesthetic-induced inhibition of collagen secretion in cultured cells under conditions where microtubules are not depolymerized by these agents. Tertiary amine local anesthetics previously have been shown to influence some microtubule-dependent cellular functions. Since several cell secretion processes, including secretion of collagen, have been shown to be inhibited by microtubule-disrupting drugs such as colchicine, we determined whether local anesthetics affect collagen secretion. Six local anesthetics inhibited collagen and non-collagen protein secretion (up to 98%) into the extracellular medium of 3T3 cells and human fibroblasts, an effect apparently independent of influences on proline transport and total protein synthesis. A combination of colchicine and cytochalasin B did not duplicate the effects of local anesthetics. The effects of subsaturating concentrations of colchicine and procaine on secretion were additive, suggesting that both drugs act on the secretory pathway at the level of microtubules, but other effects of the two types of drugs were strikingly different. In comparing the mechanisms of action of colchicine and local anesthetics, it was seen that, in contrast to colchicine, radioactive procaine and lidocaine were slowly transported into 3T3 cells, did not bind to the tubulin-containing TCA-insoluble fraction, and did not bind to purified tubulin in vitro. The fraction of cellular tubulin present as microtubules (47% in normal cells) was determined by measuring tubulin in stabilized, sedimentable microtubules compared to total tubulin, using a [3H]colchicine binding assay. Pretreatment of cells in the cold or with colchicine led to depolymerization of microtubules, but pretreatment with five local anesthetics tested did not. Therefore, in contrast to colchicine, local anesthetics in concentrations that inhibit secretion do not directly interact with or depolymerize microtubules. These drugs, however, do affect a microtubule-dependent process and may do so by detaching the microtubular system from the cell membrane."}
{"id": "PMID:479289", "title": "Stereo electron microscopy of the 25-nm chromatin fibers in isolated nuclei.", "content": "Thin sections (0.1-0.25 micron) of isolated chicken erythrocyte nuclei were examined at various tilt angles. Stereo pairs of electron micrographs document the parallel alignment of 25-nm chromatin fibers adjacent to the nuclear envelope, and demonstrate a fiber substructure consistent with close-packed arrays of nucleosomes.", "contents": "Stereo electron microscopy of the 25-nm chromatin fibers in isolated nuclei. Thin sections (0.1-0.25 micron) of isolated chicken erythrocyte nuclei were examined at various tilt angles. Stereo pairs of electron micrographs document the parallel alignment of 25-nm chromatin fibers adjacent to the nuclear envelope, and demonstrate a fiber substructure consistent with close-packed arrays of nucleosomes."}
{"id": "PMID:479290", "title": "DNase-I-dependent dissociation of erythrocyte cytoskeletons.", "content": "The human erythrocyte contains a complex of peripheral membrane proteins which forms an extensive network or cytoskeleton on the cytoplasmic membrane surface. When I treat erythrocyte cytoskeletons with deoxyribonuclease I (DNase I), the cytoskeletons dissociate and erythrocyte actin is solubilized. The dissociation of the cytoskeletons by DNase I parallels the disruption of actin filaments in vitro by DNase I and is blocked by the addition of action to the DNase I. Large protein complexes remain after DNase I disrupts the cytoskeletons, but these complexes are no longer visible in the light microscope nor sedimentable and are selectively depleted with respect to actin. From these studies, I suggest that DNase I binds to and solubilizes actin, which serves as a structural link between protein complexes in the erythrocyte cytoskeleton.", "contents": "DNase-I-dependent dissociation of erythrocyte cytoskeletons. The human erythrocyte contains a complex of peripheral membrane proteins which forms an extensive network or cytoskeleton on the cytoplasmic membrane surface. When I treat erythrocyte cytoskeletons with deoxyribonuclease I (DNase I), the cytoskeletons dissociate and erythrocyte actin is solubilized. The dissociation of the cytoskeletons by DNase I parallels the disruption of actin filaments in vitro by DNase I and is blocked by the addition of action to the DNase I. Large protein complexes remain after DNase I disrupts the cytoskeletons, but these complexes are no longer visible in the light microscope nor sedimentable and are selectively depleted with respect to actin. From these studies, I suggest that DNase I binds to and solubilizes actin, which serves as a structural link between protein complexes in the erythrocyte cytoskeleton."}
{"id": "PMID:479291", "title": "Troponin in embryonic chick skeletal muscle cells in vitro. An immunoelectron microscope study.", "content": "The fine structurel distribution of troponin on thin filaments in developing myofibrils was investigated by the use of immunoelectron microscopy. Embryonic chick skeletal muscle cells grown in vitro were treated with antibodies against each of the troponin components (troponin T, I, and C) from adult chicken muscles. Each antibody was distributed along the thin filaments with a period of 38 nm. It is concluded that these newly synthesized regulatory proteins are assembled at their characteristic position from the initial phases of myofibrillogenesis.", "contents": "Troponin in embryonic chick skeletal muscle cells in vitro. An immunoelectron microscope study. The fine structurel distribution of troponin on thin filaments in developing myofibrils was investigated by the use of immunoelectron microscopy. Embryonic chick skeletal muscle cells grown in vitro were treated with antibodies against each of the troponin components (troponin T, I, and C) from adult chicken muscles. Each antibody was distributed along the thin filaments with a period of 38 nm. It is concluded that these newly synthesized regulatory proteins are assembled at their characteristic position from the initial phases of myofibrillogenesis."}
{"id": "PMID:479292", "title": "Sperm binding and fertilization envelope formation in a cell surface complex isolated from sea urchin eggs.", "content": "An isolated surface complex consisting of the vitelline layer, plasma membrane, and attached secretory vesicles has been examined for its ability to bind sperm and to form the fertilization envelope. Isolated surface complexes (or intact eggs) fixed in glutaraldehyde and then washed in artificial sea water are capable of binding sperm in a species-specific manner. Sperm which bind to the isolated surface complex exhibit the acrosomal process only when they are associated with the exterior surface (vitelline layer) of the complex. Upon resuspension of the unfixed surface complex in artificial sea water, a limiting envelope is formed which, based on examination of thin sections and negatively stained surface preparations, is structurally similar to the fertilization envelope formed by the fertilized egg. These results suggest that the isolated egg surface complex retains the sperm receptor, as well as integrated functions for the secretion of components involved in assembly of the fertilization envelope.", "contents": "Sperm binding and fertilization envelope formation in a cell surface complex isolated from sea urchin eggs. An isolated surface complex consisting of the vitelline layer, plasma membrane, and attached secretory vesicles has been examined for its ability to bind sperm and to form the fertilization envelope. Isolated surface complexes (or intact eggs) fixed in glutaraldehyde and then washed in artificial sea water are capable of binding sperm in a species-specific manner. Sperm which bind to the isolated surface complex exhibit the acrosomal process only when they are associated with the exterior surface (vitelline layer) of the complex. Upon resuspension of the unfixed surface complex in artificial sea water, a limiting envelope is formed which, based on examination of thin sections and negatively stained surface preparations, is structurally similar to the fertilization envelope formed by the fertilized egg. These results suggest that the isolated egg surface complex retains the sperm receptor, as well as integrated functions for the secretion of components involved in assembly of the fertilization envelope."}
{"id": "PMID:479293", "title": "Organization of tubulin in normal and transformed rat kidney cells.", "content": "We have carried out a quantitative biochemical and ultrastructural study of tubulin and microtubules in a normal rat kidney (NRK) cell line and its viral transformant (442) in culture. Under equivalent culture conditions, both cell lines contain the same amount of tubulin according to a colchicine-binding assay. The normal and transformed cells differ significantly, however, with respect to the state of organization of their tubulin. Counts of microtubules in sectioned cells indicate that NRK cells have almost twice as many microtubules per unit area of cytoplasm as the 442 cells. Centrifugation studies, on the other hand, show that 442 cells have almost twice as much pelletable tubulin as the NRK cells. We propose, therefore, that the transformed cells contain a large amount of tubulin which is in some alternative aggregate form that is not morphologically detectable as microtubles in the cytoplasm", "contents": "Organization of tubulin in normal and transformed rat kidney cells. We have carried out a quantitative biochemical and ultrastructural study of tubulin and microtubules in a normal rat kidney (NRK) cell line and its viral transformant (442) in culture. Under equivalent culture conditions, both cell lines contain the same amount of tubulin according to a colchicine-binding assay. The normal and transformed cells differ significantly, however, with respect to the state of organization of their tubulin. Counts of microtubules in sectioned cells indicate that NRK cells have almost twice as many microtubules per unit area of cytoplasm as the 442 cells. Centrifugation studies, on the other hand, show that 442 cells have almost twice as much pelletable tubulin as the NRK cells. We propose, therefore, that the transformed cells contain a large amount of tubulin which is in some alternative aggregate form that is not morphologically detectable as microtubles in the cytoplasm"}
{"id": "PMID:479294", "title": "Microtrabecular lattice of the cytoplasmic ground substance. Artifact or reality.", "content": "The cytoplasmic ground substance of cultured cells prepared for high voltage transmission electron microscopy (glutaraldehyde/osmium fixed, alcohol or acetone dehydrated, critical-point dried) consists of slender (3-6 nm Diam) strands--the microtrabeculae (55)--that form an irregular three-dimensional lattice (the microtrabecular lattice). The microtrabeculae interconnect the membranous and nonmembranous organelles and are confluent with the cortices of the cytoplast. The lattice is found in all portions of the cytoplast of all cultured cells examined. The possibility that the lattice structure is an artifact of specimen preparation has been tested by (a) subjecting whole cultured cells (WI-38, NRK, chick embryo fibroblasts) to various chemical (aldehydes, osmium tetroxide) and nonchemical (freezing) fixation schedules, (b) examination of model systems (erythrocytes, protein solutions), (c) substantiating the relaibility of critical-point drying, and (d) comparing images of whole cells with conventionally prepared (plastic-embedded) cells. The lattice structure is preserved by chemical and nonchemical fixation, though alterations in ultrastructure can occur especially after prolonged exposure to osmium tetroxide. The critical-point method for drying specimens appears to be reliable as is the freeze-drying method. The discrepancies between images of plastic-embedded and sectioned cells, and images of whole, critical-point dried cells appear to be related, in part, to the electron-scattering properties of the embedding resin. The described observations indicate that the microtrabecular lattice seen in electron micrographs closely represents the nonrandom structure of the cytoplasmic ground substance of living cultured cells.", "contents": "Microtrabecular lattice of the cytoplasmic ground substance. Artifact or reality. The cytoplasmic ground substance of cultured cells prepared for high voltage transmission electron microscopy (glutaraldehyde/osmium fixed, alcohol or acetone dehydrated, critical-point dried) consists of slender (3-6 nm Diam) strands--the microtrabeculae (55)--that form an irregular three-dimensional lattice (the microtrabecular lattice). The microtrabeculae interconnect the membranous and nonmembranous organelles and are confluent with the cortices of the cytoplast. The lattice is found in all portions of the cytoplast of all cultured cells examined. The possibility that the lattice structure is an artifact of specimen preparation has been tested by (a) subjecting whole cultured cells (WI-38, NRK, chick embryo fibroblasts) to various chemical (aldehydes, osmium tetroxide) and nonchemical (freezing) fixation schedules, (b) examination of model systems (erythrocytes, protein solutions), (c) substantiating the relaibility of critical-point drying, and (d) comparing images of whole cells with conventionally prepared (plastic-embedded) cells. The lattice structure is preserved by chemical and nonchemical fixation, though alterations in ultrastructure can occur especially after prolonged exposure to osmium tetroxide. The critical-point method for drying specimens appears to be reliable as is the freeze-drying method. The discrepancies between images of plastic-embedded and sectioned cells, and images of whole, critical-point dried cells appear to be related, in part, to the electron-scattering properties of the embedding resin. The described observations indicate that the microtrabecular lattice seen in electron micrographs closely represents the nonrandom structure of the cytoplasmic ground substance of living cultured cells."}
{"id": "PMID:479295", "title": "Structural states of myelin observed by x-ray diffraction and freeze-fracture electron microscopy.", "content": "Coordinated freeze-fracture electron microscopy and x-ray diffraction were used to visualize the morphological relation between compacted and native period membrane arrays in myelinated nerves treated with dimethylsulfoxide (DMSO). Comparison of x-ray diffraction at room temperature and at low temperature was used as a critical measure of the extent of structural preservation. Our x-ray diffraction patterns show that in the presence of cryoprotective agents, it is possible to preserve with only small changes the myelin structure which exists at room temperature. These changes include a slight increase in packing disorder of the membrane, a small, negative thermal expansion of the membrane unit, and some reorganization in the cytoplasmic half of the bilayer. The freeze-fracture electron microscopy clearly demonstrates continuity of compact and native period phases in DMSO-treated myelin. Finally, the use of freezing to trap the transient, intermediate structure during a structural transition in glycerol is demonstrated.", "contents": "Structural states of myelin observed by x-ray diffraction and freeze-fracture electron microscopy. Coordinated freeze-fracture electron microscopy and x-ray diffraction were used to visualize the morphological relation between compacted and native period membrane arrays in myelinated nerves treated with dimethylsulfoxide (DMSO). Comparison of x-ray diffraction at room temperature and at low temperature was used as a critical measure of the extent of structural preservation. Our x-ray diffraction patterns show that in the presence of cryoprotective agents, it is possible to preserve with only small changes the myelin structure which exists at room temperature. These changes include a slight increase in packing disorder of the membrane, a small, negative thermal expansion of the membrane unit, and some reorganization in the cytoplasmic half of the bilayer. The freeze-fracture electron microscopy clearly demonstrates continuity of compact and native period phases in DMSO-treated myelin. Finally, the use of freezing to trap the transient, intermediate structure during a structural transition in glycerol is demonstrated."}
{"id": "PMID:479296", "title": "Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.", "content": "The receptor-rich postsynaptic membrane of the elasmobranch electric organ was fixed by quick-freezing and then viewed by freeze-fracture, deep-etching and rotary-replication. Traditional freeze-fracture revealed a distinct, geometrical pattern of shallow 8.5-nm bumps on the E fracture-face, similar to the lattice which has been seen before in chemically fixed material, but seen less clearly than after quick-freezing. Fracture plus deep-etching brought into view on the true outside of this membrane a similar geometrical pattern of 8.5-nm projections rising out of the membrane surface. The individual projections looked like structures that have been seen in negatively stained or deep-etched membrane fragments and have been identified as individual acetylcholine receptor molecules. The surface protrusions were twice as abundant as the large intramembrane particles that characterize the fracture faces of this membrane, which have also been considered to be receptor molecules. Particle counts have always been too low to match the estimates of postsynaptic receptor density derived from physiological and biochemical studies; counts of surface projections, however, more closely matched these estimates. Rotary-replication of quick-frozen, etched postsynaptic membranes enhanced the visibility of these surface protuberances and illustrated that they often occur in dimers, tetramers, and ordered rows. The variations in these surface patterns suggested that in vivo, receptors in the postsynaptic membrane may tend to pack into \"liquid crystals\" which constantly appear, flow, and disappear in the fluid environment of the membrane. Additionally, deep-etching revealed a distinct web of cytoplasmic filaments beneath the postsynaptic membrane, and revealed the basal lamina above it; and delineated possible points of contact between these structures and the membrane proper.", "contents": "Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane. The receptor-rich postsynaptic membrane of the elasmobranch electric organ was fixed by quick-freezing and then viewed by freeze-fracture, deep-etching and rotary-replication. Traditional freeze-fracture revealed a distinct, geometrical pattern of shallow 8.5-nm bumps on the E fracture-face, similar to the lattice which has been seen before in chemically fixed material, but seen less clearly than after quick-freezing. Fracture plus deep-etching brought into view on the true outside of this membrane a similar geometrical pattern of 8.5-nm projections rising out of the membrane surface. The individual projections looked like structures that have been seen in negatively stained or deep-etched membrane fragments and have been identified as individual acetylcholine receptor molecules. The surface protrusions were twice as abundant as the large intramembrane particles that characterize the fracture faces of this membrane, which have also been considered to be receptor molecules. Particle counts have always been too low to match the estimates of postsynaptic receptor density derived from physiological and biochemical studies; counts of surface projections, however, more closely matched these estimates. Rotary-replication of quick-frozen, etched postsynaptic membranes enhanced the visibility of these surface protuberances and illustrated that they often occur in dimers, tetramers, and ordered rows. The variations in these surface patterns suggested that in vivo, receptors in the postsynaptic membrane may tend to pack into \"liquid crystals\" which constantly appear, flow, and disappear in the fluid environment of the membrane. Additionally, deep-etching revealed a distinct web of cytoplasmic filaments beneath the postsynaptic membrane, and revealed the basal lamina above it; and delineated possible points of contact between these structures and the membrane proper."}
{"id": "PMID:479297", "title": "Localization of acetylcholine receptors by means of horseradish peroxidase-alpha-bungarotoxin during formation and development of the neuromuscular junction in the chick embryo.", "content": "The localization of acetylcholine receptors (AChR) in the surface of developing myogenic cells of the chick embryo anterior and posterior latissimus dorsi muscles in relation to the process of innervation has been studied at the ultrastructural level utilizing a horseradish peroxidase-alpha-bungarotoxin conjugate. Localized concentrations of AChR were found in small regions 0.1-0.4 micron in width on the surface of myogenic cells of 10- to 14-d-old muscles. Surface specializations consisting of an external coating of extraneous material and an internal accumulation of dense material are associated with the plasma membrane in the regions of AChR concentration. As the muscle fibers are innervated, reactive surface patches are found at the region of contact of the growing nerve fiber and the surface of myotubes or their fusing myoblasts. After the establishment of contact, the patches of reaction product become more numerous and coextensive within the region of the neuromuscular junction and its immediate surroundings forming a dense continuous deposit on the postsynaptic sarcolemma. Activity becomes increasingly restricted to the site of the neuromuscular junction as the embryos approach hatching. At all stages, specializations external and internal to the plasmalemma are found at regions of high density of AChR, suggesting that they play a role in the maintenance of a higher concentration of receptors at these sites. These specializations also occur at the region of initial synaptic contact, indicating that they might be recognized by the nerve and represent preferred sites of innervation. Innervation appears to exert a stabilizing influence on the area of high AChR concentration in contact with the nerve and to induce a further increase in the AChR density of this site while the number of AChR in the remaining portions of the muscle surface declines.", "contents": "Localization of acetylcholine receptors by means of horseradish peroxidase-alpha-bungarotoxin during formation and development of the neuromuscular junction in the chick embryo. The localization of acetylcholine receptors (AChR) in the surface of developing myogenic cells of the chick embryo anterior and posterior latissimus dorsi muscles in relation to the process of innervation has been studied at the ultrastructural level utilizing a horseradish peroxidase-alpha-bungarotoxin conjugate. Localized concentrations of AChR were found in small regions 0.1-0.4 micron in width on the surface of myogenic cells of 10- to 14-d-old muscles. Surface specializations consisting of an external coating of extraneous material and an internal accumulation of dense material are associated with the plasma membrane in the regions of AChR concentration. As the muscle fibers are innervated, reactive surface patches are found at the region of contact of the growing nerve fiber and the surface of myotubes or their fusing myoblasts. After the establishment of contact, the patches of reaction product become more numerous and coextensive within the region of the neuromuscular junction and its immediate surroundings forming a dense continuous deposit on the postsynaptic sarcolemma. Activity becomes increasingly restricted to the site of the neuromuscular junction as the embryos approach hatching. At all stages, specializations external and internal to the plasmalemma are found at regions of high density of AChR, suggesting that they play a role in the maintenance of a higher concentration of receptors at these sites. These specializations also occur at the region of initial synaptic contact, indicating that they might be recognized by the nerve and represent preferred sites of innervation. Innervation appears to exert a stabilizing influence on the area of high AChR concentration in contact with the nerve and to induce a further increase in the AChR density of this site while the number of AChR in the remaining portions of the muscle surface declines."}
{"id": "PMID:479298", "title": "Nexuses between the smooth muscle cells of the guinea-pig ileum.", "content": "The circular musculature of the guinea-pig ileum has been studied by freeze-fracture to analyze quantitatively the gap junctions (nexuses) between its smooth muscle cells. The average cell surface area and cell volume are 5,074 micron 2 and 3,260 micron 3. The packing density of nexuses is 48/1,000 micron 2 of cell surface or approximately 244/muscle cell. Nexuses range in area from less than 0.1 to approximately 1.5 micron 2 and they occupy 0.212% of the cell surface. The average packing density of intramembrane particles or pits in nexuses is approximately 7,200/micron 2 of nexal surface, indicating that there may be approximately 77,000 intercellular channels in the full complement of nexuses of one muscle cell.", "contents": "Nexuses between the smooth muscle cells of the guinea-pig ileum. The circular musculature of the guinea-pig ileum has been studied by freeze-fracture to analyze quantitatively the gap junctions (nexuses) between its smooth muscle cells. The average cell surface area and cell volume are 5,074 micron 2 and 3,260 micron 3. The packing density of nexuses is 48/1,000 micron 2 of cell surface or approximately 244/muscle cell. Nexuses range in area from less than 0.1 to approximately 1.5 micron 2 and they occupy 0.212% of the cell surface. The average packing density of intramembrane particles or pits in nexuses is approximately 7,200/micron 2 of nexal surface, indicating that there may be approximately 77,000 intercellular channels in the full complement of nexuses of one muscle cell."}
{"id": "PMID:479299", "title": "Characterization of the molecular components in kinetoplast-mitochondrial DNA of Trypanosoma equiperdum. Comparative study of the dyskinetoplastic and wild strains.", "content": "The structure of the kinetoplast DNA of Trypanosoma equiperdum has been studied and compared to the structure of the circular mitochondrial DNA extracted from a dyskinetoplastic strain of T. equiperdum. In T. equiperdum wild type, the kinetoplast DNA constitutes approximately 6% of the total cellular DNA and is composed of approximately 3,000 supercoiled minicircles of 6.4 x 10(5) daltons and approximately 50 circular supercoiled molecules of 15.4 x 10(6) daltons topologically interlocked; The buoyant density in CsCl of the minicircles is 1.691 g/cm 3. The large circles have a buoyant density of 1.684 g/cm 3, are homogeneous in size and are selectively cleaved by several restriction endonucleases which do not cleave the minicircles. The cleavage sites of six different restriction endonucleases have been mapped on the large circle. The minicircles are cleaved by two other restriction endonucleases, and their cleavage sites have been mapped. The mitochondrial DNA extracted from the dyskinetoplastic strain of T. equiperdum represents 7% of the total DNA of the cell and is composed of supercoiled circles, heterogeneous in size, and topologically associated in catenated oligomers. Its buoyant density in CsCl is 1.688 g/cm 3. These molecules are not cleaved by any of the eight restriction endonucleases tested. The reassociation kinetics of in vitro labeled kDNA minicircles and large circles has been studied. The results indicate that the minicircles as well as the large circles are homogeneous in sequence and that the circular DNA of the dyskinetoplastic strain has no sequence in common with the kDNA of the wild strain.", "contents": "Characterization of the molecular components in kinetoplast-mitochondrial DNA of Trypanosoma equiperdum. Comparative study of the dyskinetoplastic and wild strains. The structure of the kinetoplast DNA of Trypanosoma equiperdum has been studied and compared to the structure of the circular mitochondrial DNA extracted from a dyskinetoplastic strain of T. equiperdum. In T. equiperdum wild type, the kinetoplast DNA constitutes approximately 6% of the total cellular DNA and is composed of approximately 3,000 supercoiled minicircles of 6.4 x 10(5) daltons and approximately 50 circular supercoiled molecules of 15.4 x 10(6) daltons topologically interlocked; The buoyant density in CsCl of the minicircles is 1.691 g/cm 3. The large circles have a buoyant density of 1.684 g/cm 3, are homogeneous in size and are selectively cleaved by several restriction endonucleases which do not cleave the minicircles. The cleavage sites of six different restriction endonucleases have been mapped on the large circle. The minicircles are cleaved by two other restriction endonucleases, and their cleavage sites have been mapped. The mitochondrial DNA extracted from the dyskinetoplastic strain of T. equiperdum represents 7% of the total DNA of the cell and is composed of supercoiled circles, heterogeneous in size, and topologically associated in catenated oligomers. Its buoyant density in CsCl is 1.688 g/cm 3. These molecules are not cleaved by any of the eight restriction endonucleases tested. The reassociation kinetics of in vitro labeled kDNA minicircles and large circles has been studied. The results indicate that the minicircles as well as the large circles are homogeneous in sequence and that the circular DNA of the dyskinetoplastic strain has no sequence in common with the kDNA of the wild strain."}
{"id": "PMID:479300", "title": "Organization of neuronal microtubules in the nematode Caenorhabditis elegans.", "content": "We have studied the organization of microtubules in neurons of the nematode Caenorhabditis elegans. Six neurons, which we call the microtubule cells, contain bundles of darkly staining microtubules which can be followed easily in serial-section electron micrographs. Reconstruction of individual microtubules in these cells indicate that most, if not all, microtubules are short compared with the length of the cell process. Average microtubule length varies characteristically with cell type. The arrangement of microtubules gives an overall polarity to each bundle: the distal ends of the microtubles are on the outside of the bundle, whereas the proximal ends are preferentially inside. The distal and proximal ends each have a characteristic appearance indicating that these microtubules may have a polarity of their own. Short microtubules in processes of other neurons in C. elegans have also been observed.", "contents": "Organization of neuronal microtubules in the nematode Caenorhabditis elegans. We have studied the organization of microtubules in neurons of the nematode Caenorhabditis elegans. Six neurons, which we call the microtubule cells, contain bundles of darkly staining microtubules which can be followed easily in serial-section electron micrographs. Reconstruction of individual microtubules in these cells indicate that most, if not all, microtubules are short compared with the length of the cell process. Average microtubule length varies characteristically with cell type. The arrangement of microtubules gives an overall polarity to each bundle: the distal ends of the microtubles are on the outside of the bundle, whereas the proximal ends are preferentially inside. The distal and proximal ends each have a characteristic appearance indicating that these microtubules may have a polarity of their own. Short microtubules in processes of other neurons in C. elegans have also been observed."}
{"id": "PMID:479301", "title": "Fate of surface proteins of rabbit polymorphonuclear leukocytes during phagocytosis. I. Identification of surface proteins.", "content": "To study the fate of external membrane proteins during phagocytosis, rabbit peritoneal neutrophils were labeled by enzymatic iodination. Iodine was incorporated into at least 13 proteins ranging in size from approximately 250,000 to 18,000 daltons as judged from autoradiography of gels after SDS-polyacrylamide gel electrophoresis of labeled cells. The major contractile proteins of neutrophils, actin and myosin, were not labeled when intact cells were iodinated but were labeled when homogenates of these cells were iodinated. Nine of the iodinated proteins were released by mild protease treatment of intact cells. A plasma membrane-rich fraction was isolated by density centrifugation. This fraction was enriched at least 10-fold for lactoperoxidase-labeled acid-insoluble proteins. It was enriched to the same extent for the presence of iodinated wheat germ agglutinin that had been bound to intact cells at 4 degrees C before homogenization. Analysis of SDS-polyacrylamide gel electrophoresis revealed that the proteins of this fraction were predominantly of high molecular weight. However, only 8 of the 13 proteins iodinated on intact cells were found in this fraction. The remaining five were enriched in a dense fraction containing nuclei, intact cells, and membranous vesicles, and may represent a specialized segment of the neutrophil cell surface.", "contents": "Fate of surface proteins of rabbit polymorphonuclear leukocytes during phagocytosis. I. Identification of surface proteins. To study the fate of external membrane proteins during phagocytosis, rabbit peritoneal neutrophils were labeled by enzymatic iodination. Iodine was incorporated into at least 13 proteins ranging in size from approximately 250,000 to 18,000 daltons as judged from autoradiography of gels after SDS-polyacrylamide gel electrophoresis of labeled cells. The major contractile proteins of neutrophils, actin and myosin, were not labeled when intact cells were iodinated but were labeled when homogenates of these cells were iodinated. Nine of the iodinated proteins were released by mild protease treatment of intact cells. A plasma membrane-rich fraction was isolated by density centrifugation. This fraction was enriched at least 10-fold for lactoperoxidase-labeled acid-insoluble proteins. It was enriched to the same extent for the presence of iodinated wheat germ agglutinin that had been bound to intact cells at 4 degrees C before homogenization. Analysis of SDS-polyacrylamide gel electrophoresis revealed that the proteins of this fraction were predominantly of high molecular weight. However, only 8 of the 13 proteins iodinated on intact cells were found in this fraction. The remaining five were enriched in a dense fraction containing nuclei, intact cells, and membranous vesicles, and may represent a specialized segment of the neutrophil cell surface."}
{"id": "PMID:479302", "title": "Fate of surface proteins of rabbit polymorphonuclear leukocytes during phagocytosis. II. Internalization of proteins.", "content": "The distribution of surface proteins during phagocytosis by rabbit peritoneal polymorphonuclear leukocytes was studied to determine whether the proteins of the phagocytic vesicles of these differentiated cells were representative of the entire set of plasma membrane proteins. Phagocytosis of bovine serum albumin-diisodecylphthalate emulsion by lactoperoxidase-iodinated rabbit neutrophils was linear over 15-20 min at a rate of 96 microgram oil/min/mg cell protein. This rate was similar to that of unlabeled cells. Incorporation of cell-associated free iodine by endogenous myeloperoxidase during phagocytosis was inhibited by 1 mM cyanide, which had no effect on the rate of particle uptake. The surface of intact neutrophils contained at least 13 iodinated proteins distinguishable by polyacrylamide gel electrophoresis followed by autoradiography. Isolated phagosomes were deficient in six of these proteins. The plasma membrane fraction of these cells was missing five of these same proteins which, however, were enriched in a dense surface fraction (Willinger, M., and F. R. Frankel. J. Cell Biol. 82: 32-44). When experimental conditions were reversed, and the PMNs were labeled after phagocytosis, these five proteins remained on the cell surface, while at least three of the major proteins found on resting cells were depleted. Incubating the cells with colchicine, which has been shown to affect the distribution of some plasma membrane constituents during phagocytosis, had no effect on the distribution of surface proteins in our system. These results indicate that a nonrandom interiorization of lactoperoxidase-labeled surface proteins of polymorphonuclear leukocytes occurs during phagocytosis.", "contents": "Fate of surface proteins of rabbit polymorphonuclear leukocytes during phagocytosis. II. Internalization of proteins. The distribution of surface proteins during phagocytosis by rabbit peritoneal polymorphonuclear leukocytes was studied to determine whether the proteins of the phagocytic vesicles of these differentiated cells were representative of the entire set of plasma membrane proteins. Phagocytosis of bovine serum albumin-diisodecylphthalate emulsion by lactoperoxidase-iodinated rabbit neutrophils was linear over 15-20 min at a rate of 96 microgram oil/min/mg cell protein. This rate was similar to that of unlabeled cells. Incorporation of cell-associated free iodine by endogenous myeloperoxidase during phagocytosis was inhibited by 1 mM cyanide, which had no effect on the rate of particle uptake. The surface of intact neutrophils contained at least 13 iodinated proteins distinguishable by polyacrylamide gel electrophoresis followed by autoradiography. Isolated phagosomes were deficient in six of these proteins. The plasma membrane fraction of these cells was missing five of these same proteins which, however, were enriched in a dense surface fraction (Willinger, M., and F. R. Frankel. J. Cell Biol. 82: 32-44). When experimental conditions were reversed, and the PMNs were labeled after phagocytosis, these five proteins remained on the cell surface, while at least three of the major proteins found on resting cells were depleted. Incubating the cells with colchicine, which has been shown to affect the distribution of some plasma membrane constituents during phagocytosis, had no effect on the distribution of surface proteins in our system. These results indicate that a nonrandom interiorization of lactoperoxidase-labeled surface proteins of polymorphonuclear leukocytes occurs during phagocytosis."}
{"id": "PMID:479303", "title": "Cardiac gap junction configuration after an uncoupling treatment as a function of time.", "content": "Rabbit ventricle either was fixed in glutaraldehyde without injury (control) or was injured before fixation, presumably causing electrical uncoupling of the gap junctions. All tissue was then processed for freeze-fracture. Replicas of control gap junctions exhibited irregular packing of the P-face particles and E-face pits. Average center-to-center spacing of the particles was 10.5 nm. Tissue fixed 1-5 min after injury showed clumping of gap junctional particles and pits. Within the clumps, the particles and pits were hexagonally packed and the center-to-center spacing of the particles averaged 9.5 nm. In tissue fixed 15-30 min after injury, the clumps of gap junctional particles had coalesced into a homogeneous structure in most junctions. The packing of the particles and pits was hexagonal and the spacing of the particles averaged 9.5 nm. A few pieces of rabbit atrium were frozen without prior fixation or cryoprotection to try to assess the effect of glutarldehyde fixation on gap junction structure. In this tissue the gap junctional particles were irregularly packed and their spacing averaged 10.0 nm.", "contents": "Cardiac gap junction configuration after an uncoupling treatment as a function of time. Rabbit ventricle either was fixed in glutaraldehyde without injury (control) or was injured before fixation, presumably causing electrical uncoupling of the gap junctions. All tissue was then processed for freeze-fracture. Replicas of control gap junctions exhibited irregular packing of the P-face particles and E-face pits. Average center-to-center spacing of the particles was 10.5 nm. Tissue fixed 1-5 min after injury showed clumping of gap junctional particles and pits. Within the clumps, the particles and pits were hexagonally packed and the center-to-center spacing of the particles averaged 9.5 nm. In tissue fixed 15-30 min after injury, the clumps of gap junctional particles had coalesced into a homogeneous structure in most junctions. The packing of the particles and pits was hexagonal and the spacing of the particles averaged 9.5 nm. A few pieces of rabbit atrium were frozen without prior fixation or cryoprotection to try to assess the effect of glutarldehyde fixation on gap junction structure. In this tissue the gap junctional particles were irregularly packed and their spacing averaged 10.0 nm."}
{"id": "PMID:479304", "title": "Helical substructure of neurofilaments isolated from Myxicola and squid giant axons.", "content": "Neurofilaments purified from invertebrate giant axons have been analyzed with the electron microscope. The neurofilaments have a helical substructure which is most easily observed when the neurofilaments are partially denatured with 0.5 M KCl or 2 M urea. When the ropelike structure comprising the neurofilaments untwists, two strands 4--5.5nm in diameter can be resolved. Upon further denaturation these strands break up into rod-shaped segments and subsequently these segments roll up into amorphous globular structures. Stained, filled densities can be resolved within the strand segments, and these resemble similar structures observed within the intact neurofilaments. The strands appear to consist of protofilaments 2--2.5 nm in diameter. These observations suggest that the neurofilament is a ropelike, helical structure composed of two strands twisted tightly around each other, and they su-port the filamentous rather than the golbular model of intermediate filament structure.", "contents": "Helical substructure of neurofilaments isolated from Myxicola and squid giant axons. Neurofilaments purified from invertebrate giant axons have been analyzed with the electron microscope. The neurofilaments have a helical substructure which is most easily observed when the neurofilaments are partially denatured with 0.5 M KCl or 2 M urea. When the ropelike structure comprising the neurofilaments untwists, two strands 4--5.5nm in diameter can be resolved. Upon further denaturation these strands break up into rod-shaped segments and subsequently these segments roll up into amorphous globular structures. Stained, filled densities can be resolved within the strand segments, and these resemble similar structures observed within the intact neurofilaments. The strands appear to consist of protofilaments 2--2.5 nm in diameter. These observations suggest that the neurofilament is a ropelike, helical structure composed of two strands twisted tightly around each other, and they su-port the filamentous rather than the golbular model of intermediate filament structure."}
{"id": "PMID:479305", "title": "Identification of the subunit proteins of 10-nm neurofilaments isolated from axoplasm of squid and Myxicola giant axons.", "content": "Neurofilaments were isolated from the axoplasm of the giant axons of Myxicola infundibulum and squid. The axoplasm was fractionated by discontinuous sucrose gradient centrifugation and gel filtration on Sepharose 4B. The fractions were monitored for neurofilaments by electron microscopy. When isolated in the presence of chelating agents, the neurofilaments of Myxicola are composed almost entirely of protein subunits with mol wt of 150,000 and 160,000. Squid neurofilaments contain two major proteins with mol wt of 200,000 and 60,000. These proteins are compared with other intermediate filament proteins which have been reported in the literature.", "contents": "Identification of the subunit proteins of 10-nm neurofilaments isolated from axoplasm of squid and Myxicola giant axons. Neurofilaments were isolated from the axoplasm of the giant axons of Myxicola infundibulum and squid. The axoplasm was fractionated by discontinuous sucrose gradient centrifugation and gel filtration on Sepharose 4B. The fractions were monitored for neurofilaments by electron microscopy. When isolated in the presence of chelating agents, the neurofilaments of Myxicola are composed almost entirely of protein subunits with mol wt of 150,000 and 160,000. Squid neurofilaments contain two major proteins with mol wt of 200,000 and 60,000. These proteins are compared with other intermediate filament proteins which have been reported in the literature."}
{"id": "PMID:479306", "title": "Localization of submembranous cations to the leading end of human neutrophils during chemotaxis.", "content": "Potassium pyroantimonate was used to localize sites of bound cations in human neutrophils under conditions of random migration, stimulated random migration (chemokinesis), and directed migration (chemotaxis). The cells were placed in a standard chamber in which 0.45-micron micropore filters separated the cells from the stimulus (buffer, Escherichia coli endotoxin-activated serum or the synthetic chemotactic peptide N-formyl-Met-Leu-Phe). The small pore filters permitted pseudopod formation but impeded cell imgration through the filter. Cells examined under all conditions had electron-dense precipitates of antimonate salts in some granules. However, antimonate deposits were localized in the condensed chromatin of the nucleus during random migration and associated to a large extent with the uncondensed nuclear chromatin during chemokinesis and chemotaxis. Under conditions of chemokinesis deposition of antimonate procipitates appeared on the cytoplasmic side of the plasma membrane of neutrophils whereas under conditions of chemotaxis cation deposits beneath the cell membrane were localized to the pseudopods which were directed toward the chemoattractant. In addition to endotoxin-activated serum, concentrations of N-formyl-Met-Leu-Phe which caused neutrophil chemotaxis (10(-8) M) also caused cation deposition beneath the cell membrane at the leading end of the cell regardless of whether albumin was present in the incubation media. However, with higher concentrations of the synthetic peptide (10(-5) M) which caused granule release and were not chemotactic, submembranous cation deposition was not seen. EDTA (10 mM) and EGTA (10 mM) removed nuclear, granular, and submembranous cation deposits from neutrophils examined under conditions of chemotaxis. X-ray microprobe analysis of antimonate deposits revealed the possible presence of calcium but did not detect sodium or magnesium. The data indicate that chemotactic factors induce submembranous deposition of cations, most likely Ca++, which localize to the leading edge of cells exposed to a gradient of chemoattractant.", "contents": "Localization of submembranous cations to the leading end of human neutrophils during chemotaxis. Potassium pyroantimonate was used to localize sites of bound cations in human neutrophils under conditions of random migration, stimulated random migration (chemokinesis), and directed migration (chemotaxis). The cells were placed in a standard chamber in which 0.45-micron micropore filters separated the cells from the stimulus (buffer, Escherichia coli endotoxin-activated serum or the synthetic chemotactic peptide N-formyl-Met-Leu-Phe). The small pore filters permitted pseudopod formation but impeded cell imgration through the filter. Cells examined under all conditions had electron-dense precipitates of antimonate salts in some granules. However, antimonate deposits were localized in the condensed chromatin of the nucleus during random migration and associated to a large extent with the uncondensed nuclear chromatin during chemokinesis and chemotaxis. Under conditions of chemokinesis deposition of antimonate procipitates appeared on the cytoplasmic side of the plasma membrane of neutrophils whereas under conditions of chemotaxis cation deposits beneath the cell membrane were localized to the pseudopods which were directed toward the chemoattractant. In addition to endotoxin-activated serum, concentrations of N-formyl-Met-Leu-Phe which caused neutrophil chemotaxis (10(-8) M) also caused cation deposition beneath the cell membrane at the leading end of the cell regardless of whether albumin was present in the incubation media. However, with higher concentrations of the synthetic peptide (10(-5) M) which caused granule release and were not chemotactic, submembranous cation deposition was not seen. EDTA (10 mM) and EGTA (10 mM) removed nuclear, granular, and submembranous cation deposits from neutrophils examined under conditions of chemotaxis. X-ray microprobe analysis of antimonate deposits revealed the possible presence of calcium but did not detect sodium or magnesium. The data indicate that chemotactic factors induce submembranous deposition of cations, most likely Ca++, which localize to the leading edge of cells exposed to a gradient of chemoattractant."}
{"id": "PMID:479307", "title": "Calcium-induced asymmetrical beating of triton-demembranated sea urchin sperm flagella.", "content": "Asymmetrical bending waves can be obtained by reactivating demembranated sea urchin spermatozoa at high Ca2+ concentrations. Moving-film flash photography shows that asymmetrical flagellar bending waves are associated with premature termination of the growth of the bends in one direction (the reverse bends) while the bends in the opposite direction (the principal bends) grow for one full beat cycle, and with unequal rates of growth of principal and reverse bends. The relative proportions of these two components of asymmetry are highly variable. The increased angle in the principal bend is compensated by a decreased angle in the reverse bend, so that there is no change in mean bend angle; the wavelength and beat frequency are also independent of the degree of asymmetry. This new information is still insufficient to identify a particular mechanism for Ca2+-induced asymmetry. When a developing bend stops growing before initiation of growth of a new bend in the same direction, a modification of the sliding between tubules in the distal portion of the flagellum is required. This modification can be described as a superposition of synchronous sliding on the metachronous sliding associated with propagating bending waves. Synchronous sliding is particularly evident in highly asymmetrical flagella, but is probably not the cause of asymmetry. The control of metachronous sliding appears to be unaffected by the superposition of synchronous sliding.", "contents": "Calcium-induced asymmetrical beating of triton-demembranated sea urchin sperm flagella. Asymmetrical bending waves can be obtained by reactivating demembranated sea urchin spermatozoa at high Ca2+ concentrations. Moving-film flash photography shows that asymmetrical flagellar bending waves are associated with premature termination of the growth of the bends in one direction (the reverse bends) while the bends in the opposite direction (the principal bends) grow for one full beat cycle, and with unequal rates of growth of principal and reverse bends. The relative proportions of these two components of asymmetry are highly variable. The increased angle in the principal bend is compensated by a decreased angle in the reverse bend, so that there is no change in mean bend angle; the wavelength and beat frequency are also independent of the degree of asymmetry. This new information is still insufficient to identify a particular mechanism for Ca2+-induced asymmetry. When a developing bend stops growing before initiation of growth of a new bend in the same direction, a modification of the sliding between tubules in the distal portion of the flagellum is required. This modification can be described as a superposition of synchronous sliding on the metachronous sliding associated with propagating bending waves. Synchronous sliding is particularly evident in highly asymmetrical flagella, but is probably not the cause of asymmetry. The control of metachronous sliding appears to be unaffected by the superposition of synchronous sliding."}
{"id": "PMID:479308", "title": "Acetylcholine receptors in regenerating muscle accumulate at original synaptic sites in the absence of the nerve.", "content": "We examined the role of nerve terminals in organizing acetylcholine receptors on regenerating skeletal-muscle fibers. When muscle fibers are damaged, they degenerate and are phagocytized, but their basal lamina sheaths survive. New myofibers form within the original basal lamina sheaths, and they become innervated precisely at the original synaptic sites on the sheaths. After denervating and damaging muscle, we allowed myofibers to regenerate but deliberately prevented reinnervation. The distribution of acetylcholine receptors on regenerating myofibers was determined by histological methods, using [125I] alpha-bungarotoxin or horseradish peroxidase-alpha-bungarotoxin; original synaptic sites on the basal lamina sheaths were marked by cholinesterase stain. By one month after damage to the muscle, the new myofibers have accumulations of acetylcholine receptors that are selectively localized to the original synaptic sites. The density of the receptors at these sites is the same as at normal neuromuscular junctions. Folds in the myofiber surface resembling junctional folds at normal neuromuscular junctions also occur at original synaptic sites in the absence of nerve terminals. Our results demonstrate that the biochemical and structural organization of the subsynaptic membrane in regenerating muscle is directed by structures that remain at synaptic sites after removal of the nerve.", "contents": "Acetylcholine receptors in regenerating muscle accumulate at original synaptic sites in the absence of the nerve. We examined the role of nerve terminals in organizing acetylcholine receptors on regenerating skeletal-muscle fibers. When muscle fibers are damaged, they degenerate and are phagocytized, but their basal lamina sheaths survive. New myofibers form within the original basal lamina sheaths, and they become innervated precisely at the original synaptic sites on the sheaths. After denervating and damaging muscle, we allowed myofibers to regenerate but deliberately prevented reinnervation. The distribution of acetylcholine receptors on regenerating myofibers was determined by histological methods, using [125I] alpha-bungarotoxin or horseradish peroxidase-alpha-bungarotoxin; original synaptic sites on the basal lamina sheaths were marked by cholinesterase stain. By one month after damage to the muscle, the new myofibers have accumulations of acetylcholine receptors that are selectively localized to the original synaptic sites. The density of the receptors at these sites is the same as at normal neuromuscular junctions. Folds in the myofiber surface resembling junctional folds at normal neuromuscular junctions also occur at original synaptic sites in the absence of nerve terminals. Our results demonstrate that the biochemical and structural organization of the subsynaptic membrane in regenerating muscle is directed by structures that remain at synaptic sites after removal of the nerve."}
{"id": "PMID:479309", "title": "Fate of plasma membrane during endocytosis. I. Uptake and processing of anti-plasma membrane and control immunoglobulins by cultured fibroblasts.", "content": "The uptake and processing by cultured rat embryo fibroblasts of control rabbit immunoglobulins (C IgG) or IgG directed against plasma membrane constituents (anti-PM IgG), and labeled with fluorescein (F) or with radioactive acetate (A), have been investigated by cell fractionation and immunological techniques. Both F and A anti-PM IgGs become bound to the cell surface, by a process that is slow, but largely temperature-independent. In the presence of an excess of high-affinity antibodies, binding reaches an absolute limit which corresponds to extensive coating of the plasma membrane. The anti-PM IgGs remain attached to the membrane for at least several days, even at 37 degrees C, with no significant transfer to lysosomes or degradation. In contrast, C IgGs are handled very differently by the fibroblasts, and their fate is strikingly affected by the type of labeling used. AC IgG is taken up slowly, at a rate proportional to its concentration, and is subsequently broken down in what appears to be lysosomes. Part of the AC IgG also binds to the plasma membrane. FC IgG is taken up many times faster than AC IgG, though with the same strict linearity as a function of concentration. Most of the FC IgG taken up is stored in cytoplasmic granules which behave like lysosomes. For reasons that are not understood, only about half of the stored FC IgG can be broken down. Cells exposed simulatnaously to AC IgG and FC IgG, or to A anti-PM IgG and FC IgG, handle each type of IgG in its characteristic fashion. Kinetic analysis of these results indicates that Ac IgG could be taken up by fluid endocytosis, but that FC IgG must be interiorized by a selective mechanism, presumably adsorptive in nature. That anti-PM antibodies remain stably bound to the plasma membrane and do not interfere with the uptake of FC IgG is interpreted to indicate either that two distinct membrane domains are involved in the two phenomena, or that membrane patches coated with anti-PM IgG participate in endocytosis, and are recycled back to the cell surface after delivering their contents intracellularly.", "contents": "Fate of plasma membrane during endocytosis. I. Uptake and processing of anti-plasma membrane and control immunoglobulins by cultured fibroblasts. The uptake and processing by cultured rat embryo fibroblasts of control rabbit immunoglobulins (C IgG) or IgG directed against plasma membrane constituents (anti-PM IgG), and labeled with fluorescein (F) or with radioactive acetate (A), have been investigated by cell fractionation and immunological techniques. Both F and A anti-PM IgGs become bound to the cell surface, by a process that is slow, but largely temperature-independent. In the presence of an excess of high-affinity antibodies, binding reaches an absolute limit which corresponds to extensive coating of the plasma membrane. The anti-PM IgGs remain attached to the membrane for at least several days, even at 37 degrees C, with no significant transfer to lysosomes or degradation. In contrast, C IgGs are handled very differently by the fibroblasts, and their fate is strikingly affected by the type of labeling used. AC IgG is taken up slowly, at a rate proportional to its concentration, and is subsequently broken down in what appears to be lysosomes. Part of the AC IgG also binds to the plasma membrane. FC IgG is taken up many times faster than AC IgG, though with the same strict linearity as a function of concentration. Most of the FC IgG taken up is stored in cytoplasmic granules which behave like lysosomes. For reasons that are not understood, only about half of the stored FC IgG can be broken down. Cells exposed simulatnaously to AC IgG and FC IgG, or to A anti-PM IgG and FC IgG, handle each type of IgG in its characteristic fashion. Kinetic analysis of these results indicates that Ac IgG could be taken up by fluid endocytosis, but that FC IgG must be interiorized by a selective mechanism, presumably adsorptive in nature. That anti-PM antibodies remain stably bound to the plasma membrane and do not interfere with the uptake of FC IgG is interpreted to indicate either that two distinct membrane domains are involved in the two phenomena, or that membrane patches coated with anti-PM IgG participate in endocytosis, and are recycled back to the cell surface after delivering their contents intracellularly."}
{"id": "PMID:479310", "title": "Fate of plasma membrane during endocytosis. II. Evidence for recycling (shuttle) of plasma membrane constituents.", "content": "Cultured rat embryo fibroblasts were first allowed to store for 24 h fluorescein-labeled goat immunoglobulins directed against rabbit immunoglobulins (F anti-R IgG), and were subsequently exposed for 24 h to [(3)H]acetylated rabbit immunoglobulins known to bind to the cell membrane either specifically (anti-plasma membrane IgG: A anti-PM IgG) or unspecifically (contol IgG: AC IgG). As a result of immunological interaction between the two antibodies (no effect was found if the cells had been preloaded with control goat FC IgG), a substantial portion of the stored F anti-R IgG was unloaded from its intracellular storage site, appearing in the medium in the form of soluble immune complexes with rabbit A IgG. Part of the unloaded F anti-R IgG also was recovered in association with the plasma membrane, but only when A anti-PM IgG was used. In addition, significant reverse translocation of AC IgG from plasma membrane to lysosomes or some related intracellular storage compartment was also observed. With A anti-PM IgG, this translocation was less marked and affecte at the same time the plasma membrane marker 5'- nucleotidase. Cells that had stored horseradish peroxidase (HRP) simultaneously with F anti-R IgG did not unload HRP when exposed to A anti-PM IgG. These results support strongly, though not unequivocally, the concept that plasma membrane patches interiorized by endocytosis are recycled, or shuttled, back to the cell surface. In the framework of this concept, recycling antibody-coated membrane is taken to serve as vehicle for the selective intracellular capture and extracellular discharge of immunologically bound F anti-R IgG. The alternative explanation of regurgitation triggered off by immune complexes is considered less likely in view of the lack of HRP unloading.", "contents": "Fate of plasma membrane during endocytosis. II. Evidence for recycling (shuttle) of plasma membrane constituents. Cultured rat embryo fibroblasts were first allowed to store for 24 h fluorescein-labeled goat immunoglobulins directed against rabbit immunoglobulins (F anti-R IgG), and were subsequently exposed for 24 h to [(3)H]acetylated rabbit immunoglobulins known to bind to the cell membrane either specifically (anti-plasma membrane IgG: A anti-PM IgG) or unspecifically (contol IgG: AC IgG). As a result of immunological interaction between the two antibodies (no effect was found if the cells had been preloaded with control goat FC IgG), a substantial portion of the stored F anti-R IgG was unloaded from its intracellular storage site, appearing in the medium in the form of soluble immune complexes with rabbit A IgG. Part of the unloaded F anti-R IgG also was recovered in association with the plasma membrane, but only when A anti-PM IgG was used. In addition, significant reverse translocation of AC IgG from plasma membrane to lysosomes or some related intracellular storage compartment was also observed. With A anti-PM IgG, this translocation was less marked and affecte at the same time the plasma membrane marker 5'- nucleotidase. Cells that had stored horseradish peroxidase (HRP) simultaneously with F anti-R IgG did not unload HRP when exposed to A anti-PM IgG. These results support strongly, though not unequivocally, the concept that plasma membrane patches interiorized by endocytosis are recycled, or shuttled, back to the cell surface. In the framework of this concept, recycling antibody-coated membrane is taken to serve as vehicle for the selective intracellular capture and extracellular discharge of immunologically bound F anti-R IgG. The alternative explanation of regurgitation triggered off by immune complexes is considered less likely in view of the lack of HRP unloading."}
{"id": "PMID:479311", "title": "Binding of hyaluronate to the surface of cultured cells.", "content": "The binding of hyaluronate to SV-3T3 cells was measured by incubating a suspension of cells (released from the substratum with EDTA) with 3H-labeled hyaluronate and then applying the suspension to glass fiber filters which retained the cells and the bound hyaluronate. The extent of binding was a function of both the concentration of labeled hyaluronate and the cell number. Most of the binding took place within the first 2 min of the incubation and was not influenced by the presence or absence of divalent cations. The binding of labeled hyaluronate to SV-3T3 cells could be prevented by the addition of an excess of unlabeled hyaluronate. High molecular weight preparations of hyaluronate were more effective in preventing binding than low molecular weight preparations. The binding of [3H]hyaluronate was inhibited by high concentrations of oligosaccharide fragments of hyaluronate consisting of six sugars or more, and by chondroitin. The sulfated glycosaminoglycans (chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate, heparin, and heparan sulfate) had little or no effect on the binding. The labeled hyaluronate bound to the cells could be totally removed by incubating the cells with testicular hyaluronidase, streptomyces hyaluronidase, or trypsin, indicating that the hyaluronate-binding sites are located on the cell surface.", "contents": "Binding of hyaluronate to the surface of cultured cells. The binding of hyaluronate to SV-3T3 cells was measured by incubating a suspension of cells (released from the substratum with EDTA) with 3H-labeled hyaluronate and then applying the suspension to glass fiber filters which retained the cells and the bound hyaluronate. The extent of binding was a function of both the concentration of labeled hyaluronate and the cell number. Most of the binding took place within the first 2 min of the incubation and was not influenced by the presence or absence of divalent cations. The binding of labeled hyaluronate to SV-3T3 cells could be prevented by the addition of an excess of unlabeled hyaluronate. High molecular weight preparations of hyaluronate were more effective in preventing binding than low molecular weight preparations. The binding of [3H]hyaluronate was inhibited by high concentrations of oligosaccharide fragments of hyaluronate consisting of six sugars or more, and by chondroitin. The sulfated glycosaminoglycans (chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate, heparin, and heparan sulfate) had little or no effect on the binding. The labeled hyaluronate bound to the cells could be totally removed by incubating the cells with testicular hyaluronidase, streptomyces hyaluronidase, or trypsin, indicating that the hyaluronate-binding sites are located on the cell surface."}
{"id": "PMID:479312", "title": "Replication of mammalian DNA in vitro. Evidence for initiation from fiber autoradiography.", "content": "We have used fiber autoradiography to examine the DNA product made in vitro in a lysed cell system. CHO cells were treated with 0.01% Brij-58 and the lysates were incubated at 30 degrees C in a complete reaction mixture for in vitro DNA synthesis with [3H]thymidine triphosphate ([3H]TTP) as the radioactive tracer. Fiber autoradiograms prepared from the DNA showed that it was synthesized on tandemly arranged replication units that were of average size of 20 micrometers, very similar to the size of units found in vivo. The rate of replication fork movement was 25--50% of the in vivo rate. More than 80% of forks stopped functioning by 15 min, and 95% stopped by 60 min. This suggests that synthesis is halted by premature terminations. Evidence for new initiations was provided by replication units with labeled origins in DNA synthesized in an in vitro reaction in which radioactivity was omitted for the first 10 min of incubation. This, plus the observations that the distance between initiation points (replication unit size) is not increased and that premature termination accounts largely for the cessation of synthesis, suggest that significant initiation takes place in this in vitro replication system.", "contents": "Replication of mammalian DNA in vitro. Evidence for initiation from fiber autoradiography. We have used fiber autoradiography to examine the DNA product made in vitro in a lysed cell system. CHO cells were treated with 0.01% Brij-58 and the lysates were incubated at 30 degrees C in a complete reaction mixture for in vitro DNA synthesis with [3H]thymidine triphosphate ([3H]TTP) as the radioactive tracer. Fiber autoradiograms prepared from the DNA showed that it was synthesized on tandemly arranged replication units that were of average size of 20 micrometers, very similar to the size of units found in vivo. The rate of replication fork movement was 25--50% of the in vivo rate. More than 80% of forks stopped functioning by 15 min, and 95% stopped by 60 min. This suggests that synthesis is halted by premature terminations. Evidence for new initiations was provided by replication units with labeled origins in DNA synthesized in an in vitro reaction in which radioactivity was omitted for the first 10 min of incubation. This, plus the observations that the distance between initiation points (replication unit size) is not increased and that premature termination accounts largely for the cessation of synthesis, suggest that significant initiation takes place in this in vitro replication system."}
{"id": "PMID:479313", "title": "Clusters of intramembrane particles associated with binding sites for alpha-bungarotoxin in cultured chick myotubes.", "content": "Developing chick myotubes in tissue culture were freeze-fractured to yield complementary replicas of large areas of membrane. Regions of muscle fibers with high concentrations of acetylcholine receptors were identified by binding of fluorescent-labeled alpha-bungarotoxin. Membranes in such regions contained clusters of large (100 A Diam) angular particles, similar in appearance to particles found in postsynaptic membranes of cholinergic synapses. Particles appeared in apposing areas of cytoplasmic and external leaflets but were more prevalent in the cytoplasmic leaflet. The areas of high particle concentration were coextensive with the fluorescence due to bound toxin. Treatment of cultures with tetrodotoxin increased the size of fluorescent spots and areas of high concentration of particles relative to those found in control cultures. In muscle cultures grown in the presence of spinal cord explants, some neurites contacted and innervated nearby myotubes. Intense fluorescence due to binding or alpha-bungarotoxin was present at portions of such neurite-myotube contacts. At these same portions, a high concentration of large angular particles was present in the sarcolemma adjacent to the neurite. In addition, an ordered arrangement of large particles was seen in the cytoplasmic leaflet of the neuronal plasmalemma directly apposing the muscle. The possible significance of these arrangements is discussed. Clusters on myotubes tended to be larger (contain more particles) when they occurred in groups, defined as three or more clusters with an intercluster distance of less than 0.5 micrometers. Clusters were also larger in myotubes treated with tetrodotoxin and in myotubes adjacent to some neurites in nerve-muscle cocultures. Several depressions containing particles similar to those in the clusters were found in the sarcolemma. The implications of these depressions are discussed in light of current theories of incorporation of proteins into cell membranes.", "contents": "Clusters of intramembrane particles associated with binding sites for alpha-bungarotoxin in cultured chick myotubes. Developing chick myotubes in tissue culture were freeze-fractured to yield complementary replicas of large areas of membrane. Regions of muscle fibers with high concentrations of acetylcholine receptors were identified by binding of fluorescent-labeled alpha-bungarotoxin. Membranes in such regions contained clusters of large (100 A Diam) angular particles, similar in appearance to particles found in postsynaptic membranes of cholinergic synapses. Particles appeared in apposing areas of cytoplasmic and external leaflets but were more prevalent in the cytoplasmic leaflet. The areas of high particle concentration were coextensive with the fluorescence due to bound toxin. Treatment of cultures with tetrodotoxin increased the size of fluorescent spots and areas of high concentration of particles relative to those found in control cultures. In muscle cultures grown in the presence of spinal cord explants, some neurites contacted and innervated nearby myotubes. Intense fluorescence due to binding or alpha-bungarotoxin was present at portions of such neurite-myotube contacts. At these same portions, a high concentration of large angular particles was present in the sarcolemma adjacent to the neurite. In addition, an ordered arrangement of large particles was seen in the cytoplasmic leaflet of the neuronal plasmalemma directly apposing the muscle. The possible significance of these arrangements is discussed. Clusters on myotubes tended to be larger (contain more particles) when they occurred in groups, defined as three or more clusters with an intercluster distance of less than 0.5 micrometers. Clusters were also larger in myotubes treated with tetrodotoxin and in myotubes adjacent to some neurites in nerve-muscle cocultures. Several depressions containing particles similar to those in the clusters were found in the sarcolemma. The implications of these depressions are discussed in light of current theories of incorporation of proteins into cell membranes."}
{"id": "PMID:479314", "title": "Sensory adaptation of leukocytes to chemotactic peptides.", "content": "The morphology and behavior of polymorphonuclear leukocytes (PMNs) were studied after rapid changes in the concentration of a chemotactic factor N-formylnorleucylleucylphenylalanine (f-NorleuLeuPhe) (Schiffmann et al., 1975, Proc. Natl. Acad. Sci. U. S. A. 72:1059--1062). After an increase in peptide concentration, the cells round, form lamellipodia or ruffles over most of their surface, and stop locomotion. These changes are transient. After a delay, the cells, still in the presence of peptide, withdraw most of the ruffles and resume locomotion, forming ruffles only at their front. Cells repeat the transient generalized ruffling upon further increase in peptide concentration. The behavioral changes occur over the same dose range as binding to a saturable receptor. The duration of the transient response after a concentration increase is roughly proportional to the increase in the number of cell receptors occupied as a result of the concentration change. Decreasing the concentration of peptide causes the cells to round transiently and form blebs before they recommence locomotion. The transient nature of these aspects of the cell's responsiveness to chemotactic factors appears to be due to adaptation by the cells. The ability to adapt to the concentration of a chemotactic factor may be important in leukocyte chemotaxis.", "contents": "Sensory adaptation of leukocytes to chemotactic peptides. The morphology and behavior of polymorphonuclear leukocytes (PMNs) were studied after rapid changes in the concentration of a chemotactic factor N-formylnorleucylleucylphenylalanine (f-NorleuLeuPhe) (Schiffmann et al., 1975, Proc. Natl. Acad. Sci. U. S. A. 72:1059--1062). After an increase in peptide concentration, the cells round, form lamellipodia or ruffles over most of their surface, and stop locomotion. These changes are transient. After a delay, the cells, still in the presence of peptide, withdraw most of the ruffles and resume locomotion, forming ruffles only at their front. Cells repeat the transient generalized ruffling upon further increase in peptide concentration. The behavioral changes occur over the same dose range as binding to a saturable receptor. The duration of the transient response after a concentration increase is roughly proportional to the increase in the number of cell receptors occupied as a result of the concentration change. Decreasing the concentration of peptide causes the cells to round transiently and form blebs before they recommence locomotion. The transient nature of these aspects of the cell's responsiveness to chemotactic factors appears to be due to adaptation by the cells. The ability to adapt to the concentration of a chemotactic factor may be important in leukocyte chemotaxis."}
{"id": "PMID:479315", "title": "Micromanipulation studies of chromosome movement. I. Chromosome-spindle attachment and the mechanical properties of chromosomal spindle fibers.", "content": "We have used micromanipulation to study the attachment of chromosomes to the spindle and the mechanical properties of the chromosomal spindle fibers. Individual chromosomes can be displaced about the periphery of the spindle, in the plane of the metaphase plate, without altering the structure of the spindle or the positions of the nonmanipulated chromosomes. From mid-prometaphase through the onset of anaphase, chromosomes resist displacement toward either spindle pole, or beyond the spindle periphery. In anaphase a chromosome can be displaced either toward its spindle pole or laterally, beyond the periphery of the spindle; however, the chromosome resists displacement away from the spindle pole. When an anaphase half-bivalent is displaced toward its spindle pole, it stops migrating until the nonmanipulated half-bivalents reach a similar distance from the pole. The manipulated half-bivalent then resumes its poleward migration at the normal anaphase rate. No evidence was found for mechanical attachments between separating half-bivalents in anaphase. Our observations demonstrate that chromosomes are individually anchored to the spindle by fibers which connect the kinetochores of the chromosomes to the spindle poles. These fibers are flexible, much less extensible than the chromosomes, and are to pivot about their attachment points. While the fibers are able to support a tensile force sufficient to stretch a chromosome, they buckle when subjected to a compressive force. Preliminary evidence suggests that the mechanical attachment fibers detected with micromanipulation correspond to the birefringent chromosomal spindle fibers observed with polarization microscopy.", "contents": "Micromanipulation studies of chromosome movement. I. Chromosome-spindle attachment and the mechanical properties of chromosomal spindle fibers. We have used micromanipulation to study the attachment of chromosomes to the spindle and the mechanical properties of the chromosomal spindle fibers. Individual chromosomes can be displaced about the periphery of the spindle, in the plane of the metaphase plate, without altering the structure of the spindle or the positions of the nonmanipulated chromosomes. From mid-prometaphase through the onset of anaphase, chromosomes resist displacement toward either spindle pole, or beyond the spindle periphery. In anaphase a chromosome can be displaced either toward its spindle pole or laterally, beyond the periphery of the spindle; however, the chromosome resists displacement away from the spindle pole. When an anaphase half-bivalent is displaced toward its spindle pole, it stops migrating until the nonmanipulated half-bivalents reach a similar distance from the pole. The manipulated half-bivalent then resumes its poleward migration at the normal anaphase rate. No evidence was found for mechanical attachments between separating half-bivalents in anaphase. Our observations demonstrate that chromosomes are individually anchored to the spindle by fibers which connect the kinetochores of the chromosomes to the spindle poles. These fibers are flexible, much less extensible than the chromosomes, and are to pivot about their attachment points. While the fibers are able to support a tensile force sufficient to stretch a chromosome, they buckle when subjected to a compressive force. Preliminary evidence suggests that the mechanical attachment fibers detected with micromanipulation correspond to the birefringent chromosomal spindle fibers observed with polarization microscopy."}
{"id": "PMID:479316", "title": "Micromanipulation studies of chromosome movement. II. Birefringent chromosomal fibers and the mechanical attachment of chromosomes to the spindle.", "content": "The degree of mechanical coupling of chromosomes to the spindles of Nephrotoma and Trimeratropis primary spermatocytes varies with the stage of meiosis and the birefringent retardation of the chromosomal fibers. In early prometaphase, before birefringent chromosomal fibers have formed, a bivalent can be displaced toward a spindle pole by a single, continuous pull with a microneedle. Resistance to poleward displacement increases with increased development of the chromosomal fibers, reaching a maximum at metaphase. At this stage kinetochores cannot be displaced greater than 1 micrometer toward either spindle pole, even by a force which is sufficient to displace the entire spindle within the cell. The abolition of birefringence with either colcemid or vinblastine results in the loss of chromosome-spindle attachment. In the absence of birefringent fibers a chromosome can be displaced anywhere within the cell. The photochemical inactivation of colcemid by irradiation with 366-nm light results in the reformation of birefringent chromosomal fibers and the concomitant re-establishment of chromosome attachment to the spindle. These results support the hypothesis that the birefringent chromosomal fibers anchor the chromosomes to the spindle and transmit the force for anaphase chromosome movement.", "contents": "Micromanipulation studies of chromosome movement. II. Birefringent chromosomal fibers and the mechanical attachment of chromosomes to the spindle. The degree of mechanical coupling of chromosomes to the spindles of Nephrotoma and Trimeratropis primary spermatocytes varies with the stage of meiosis and the birefringent retardation of the chromosomal fibers. In early prometaphase, before birefringent chromosomal fibers have formed, a bivalent can be displaced toward a spindle pole by a single, continuous pull with a microneedle. Resistance to poleward displacement increases with increased development of the chromosomal fibers, reaching a maximum at metaphase. At this stage kinetochores cannot be displaced greater than 1 micrometer toward either spindle pole, even by a force which is sufficient to displace the entire spindle within the cell. The abolition of birefringence with either colcemid or vinblastine results in the loss of chromosome-spindle attachment. In the absence of birefringent fibers a chromosome can be displaced anywhere within the cell. The photochemical inactivation of colcemid by irradiation with 366-nm light results in the reformation of birefringent chromosomal fibers and the concomitant re-establishment of chromosome attachment to the spindle. These results support the hypothesis that the birefringent chromosomal fibers anchor the chromosomes to the spindle and transmit the force for anaphase chromosome movement."}
{"id": "PMID:479317", "title": "Microtubule initiation at kinetochores and centrosomes in lysed mitotic cells. Inhibition of site-specific nucleation by tubulin antibody.", "content": "A lysed cell system was developed to determine whether tubulin antibody can block the nucleation of exogenous tubulin at kinetochores and centrosomes. Mitotic PtK2 cells were pretreated with colcemid to remove all endogenous microtubules and were lysed with Triton X-100 in PIPES-EGTA-Mg++ buffer. This procedure left centrosomes, chromosomes, and kinetochores intact as determined by electron microscopy of thin-sectioned cells. Exposure of the lysed cells to phorphocellulose-purified tubulin dimers at 37 degrees C in the presence of 1 mM GTP resulted in site-specific nucleation of microtubules at centrosomes and kinetochores. Treatment of the lysed cell preparations with tubulin antibody before subsequent exposure to the exogenous tubulin resulted in almost complete blockage of microtubule nucleation, especially at kinetochores. Pretreatment of the lysed cell preparations with control antibody or buffer without antibody had no effect on the ability of centrosomes and kinetochores to initiate microtubule assembly. The implications of these results with respect to the molecular composition of centrosomes and kinetochores are discussed.", "contents": "Microtubule initiation at kinetochores and centrosomes in lysed mitotic cells. Inhibition of site-specific nucleation by tubulin antibody. A lysed cell system was developed to determine whether tubulin antibody can block the nucleation of exogenous tubulin at kinetochores and centrosomes. Mitotic PtK2 cells were pretreated with colcemid to remove all endogenous microtubules and were lysed with Triton X-100 in PIPES-EGTA-Mg++ buffer. This procedure left centrosomes, chromosomes, and kinetochores intact as determined by electron microscopy of thin-sectioned cells. Exposure of the lysed cells to phorphocellulose-purified tubulin dimers at 37 degrees C in the presence of 1 mM GTP resulted in site-specific nucleation of microtubules at centrosomes and kinetochores. Treatment of the lysed cell preparations with tubulin antibody before subsequent exposure to the exogenous tubulin resulted in almost complete blockage of microtubule nucleation, especially at kinetochores. Pretreatment of the lysed cell preparations with control antibody or buffer without antibody had no effect on the ability of centrosomes and kinetochores to initiate microtubule assembly. The implications of these results with respect to the molecular composition of centrosomes and kinetochores are discussed."}
{"id": "PMID:479318", "title": "Blue light-induced, intrinsic vacuolar fluorescence in onion guard cells.", "content": "Guard cells of onion irradiated with broad-band blue light display a green intrinsic fluorescence. The fluorescence has been found in eleven species of Allium, but it has not been observed in any other monocot or dicot examined. The fluorescence occurs only in guard cells and is absent in neighbouring epidermal cells. During development it is first apparent in guard mother cells soon after the asymmetric division. Microscopic observation reveals that the fluorescence is associated with the vacuole and examination of vacuoles isolated from guard cell protoplasts suggests that it may be localized on the tonoplast. Microspectrophotometric analysis of single cells reveals an emission peak at around 520 nm. Our results are consistent with the view that this blue light receptor is a flavin or flavoprotein and that it might be related to the blue light-enhanced stomatal opening observed in onion.", "contents": "Blue light-induced, intrinsic vacuolar fluorescence in onion guard cells. Guard cells of onion irradiated with broad-band blue light display a green intrinsic fluorescence. The fluorescence has been found in eleven species of Allium, but it has not been observed in any other monocot or dicot examined. The fluorescence occurs only in guard cells and is absent in neighbouring epidermal cells. During development it is first apparent in guard mother cells soon after the asymmetric division. Microscopic observation reveals that the fluorescence is associated with the vacuole and examination of vacuoles isolated from guard cell protoplasts suggests that it may be localized on the tonoplast. Microspectrophotometric analysis of single cells reveals an emission peak at around 520 nm. Our results are consistent with the view that this blue light receptor is a flavin or flavoprotein and that it might be related to the blue light-enhanced stomatal opening observed in onion."}
{"id": "PMID:479319", "title": "On the differential divisions and preprophase microtubule bands involved in the development of stomata of Vigna sinensis L.", "content": "The manifestation of premitotic cell polarity and the resultant structural asymmetry of the differential divisions participating in the development of stomata of Vigna sinensis vary considerably. However, two morphologically distinct types of differential division were distinguished: (a) 'asymmetrical differential divisions', in which the premitotic polarization of the cell, the eccentric position of the nucleus during division and the differences in size and organization of the daughter cells are obvious; and (b) differential divisions in which the above features are inconspicuous or almost absent. The former occur in the ordinary protodermal cells, the latter in some meristemoids. The organization of a sharply demarcated preprophase microtubule band (PMB) precedes, all differential and non-differential divisions. In the first type of differential division the PMB is formed eccentrically, while in the second it may display either an approximately symmetrical or a clearly asymmetrical disposition, always indicating with surprising accuracy the sites where the succeeding cell plate will join the parent walls. The PMB foreshadowing the highly curved cell plates in meristemoids I of the mesoperigenous process, as well as in meristemoids I and II of the mesogenous one, are apposed only on one anticlinal wall and therefore do not encircle the nucleus or traverse the cell. In the symmetrical divisions of guard cell mother cells (GMC), as well as in those of protodermal cells, the PMB runs right round the internal plasmalemma surface in an equatorial position, coinciding with that of the future cell plate. In the former cells the wall abutting the cortical cytoplasm traversed by the band becomes locally thickened. The variability in the pattern of the microtubules of the band along the walls of the GMC is directly mirrored in the pattern of the thickening. It seems that in GMC the PMB mediates a directed exocytosis of dictyosome vesicles. In contrast to what is now generally accepted in dicotyledonous plants, each meristemoid I of both the mesogenous and mesoperigenous stomata in Vigna sinensis leaves does not inhibit but induces the formation of other meristemoids close to it.", "contents": "On the differential divisions and preprophase microtubule bands involved in the development of stomata of Vigna sinensis L. The manifestation of premitotic cell polarity and the resultant structural asymmetry of the differential divisions participating in the development of stomata of Vigna sinensis vary considerably. However, two morphologically distinct types of differential division were distinguished: (a) 'asymmetrical differential divisions', in which the premitotic polarization of the cell, the eccentric position of the nucleus during division and the differences in size and organization of the daughter cells are obvious; and (b) differential divisions in which the above features are inconspicuous or almost absent. The former occur in the ordinary protodermal cells, the latter in some meristemoids. The organization of a sharply demarcated preprophase microtubule band (PMB) precedes, all differential and non-differential divisions. In the first type of differential division the PMB is formed eccentrically, while in the second it may display either an approximately symmetrical or a clearly asymmetrical disposition, always indicating with surprising accuracy the sites where the succeeding cell plate will join the parent walls. The PMB foreshadowing the highly curved cell plates in meristemoids I of the mesoperigenous process, as well as in meristemoids I and II of the mesogenous one, are apposed only on one anticlinal wall and therefore do not encircle the nucleus or traverse the cell. In the symmetrical divisions of guard cell mother cells (GMC), as well as in those of protodermal cells, the PMB runs right round the internal plasmalemma surface in an equatorial position, coinciding with that of the future cell plate. In the former cells the wall abutting the cortical cytoplasm traversed by the band becomes locally thickened. The variability in the pattern of the microtubules of the band along the walls of the GMC is directly mirrored in the pattern of the thickening. It seems that in GMC the PMB mediates a directed exocytosis of dictyosome vesicles. In contrast to what is now generally accepted in dicotyledonous plants, each meristemoid I of both the mesogenous and mesoperigenous stomata in Vigna sinensis leaves does not inhibit but induces the formation of other meristemoids close to it."}
{"id": "PMID:479320", "title": "Increase in macromolecular amounts during the cell cycle of Tetrahymena: a contribution to cell cycle control.", "content": "Increases in RNA, protein and cell size were determined cytophotometrically during the cell division cycle of Tetrahymena. For these parameters different patterns were found. RNA accumulates slowly during G1 period and faster during macronuclear S. This agrees with the changing uridine incorporation rate which is at least partly related to the varying macronuclear DNA amount. Increases in protein content and cell size occur mainly during G1 and G2. This pattern was confirmed by determining the RNA : protein ratio in individual cells. It is minimal at the end of the G1 period. These findings and evidence from the literature suggest that initiation of DNA replication is under negative control by the relative RNA content of the cell.", "contents": "Increase in macromolecular amounts during the cell cycle of Tetrahymena: a contribution to cell cycle control. Increases in RNA, protein and cell size were determined cytophotometrically during the cell division cycle of Tetrahymena. For these parameters different patterns were found. RNA accumulates slowly during G1 period and faster during macronuclear S. This agrees with the changing uridine incorporation rate which is at least partly related to the varying macronuclear DNA amount. Increases in protein content and cell size occur mainly during G1 and G2. This pattern was confirmed by determining the RNA : protein ratio in individual cells. It is minimal at the end of the G1 period. These findings and evidence from the literature suggest that initiation of DNA replication is under negative control by the relative RNA content of the cell."}
{"id": "PMID:479321", "title": "An experimental analysis of bivalent interlocking in spermatocytes of the new Triturus vulgaris.", "content": "Interlocked bivalents at 1st meiotic metaphase are relatively uncommon in spermatocytes of the newt Triturus vulgaris, but their frequency of occurrence can be significantly increased by subjecting newts to a 24-h heat shock. Newt spermatocytes are sensitive to a heart shock at any stage between the end of premeiotic S and mid to late pachytene. The heat shock does not cause evidence desynapsis, nor does it significantly affect chiasma frequency; therefore the interlocked condition induced in spermatocytes which were subjected to a heat shock when they were in zygotene or pachytene is unlikely to be a consequence of synaptic trapping. By way of explanation it is suggested that a heat shock may cause telomers to detach from the nuclear membrane, or from the synaptonemal complex where the latter is attached to the membrane, thus allowing non-homologous chromonemata to become interwined before chiasmata have formed. If this explanation is valid, it is then further suggested that the recombination process which results in chiasma formation probably takes place in chromosomal regions lying outside the synaptonemal complex, rather than inside, between its 2 lateral elements.", "contents": "An experimental analysis of bivalent interlocking in spermatocytes of the new Triturus vulgaris. Interlocked bivalents at 1st meiotic metaphase are relatively uncommon in spermatocytes of the newt Triturus vulgaris, but their frequency of occurrence can be significantly increased by subjecting newts to a 24-h heat shock. Newt spermatocytes are sensitive to a heart shock at any stage between the end of premeiotic S and mid to late pachytene. The heat shock does not cause evidence desynapsis, nor does it significantly affect chiasma frequency; therefore the interlocked condition induced in spermatocytes which were subjected to a heat shock when they were in zygotene or pachytene is unlikely to be a consequence of synaptic trapping. By way of explanation it is suggested that a heat shock may cause telomers to detach from the nuclear membrane, or from the synaptonemal complex where the latter is attached to the membrane, thus allowing non-homologous chromonemata to become interwined before chiasmata have formed. If this explanation is valid, it is then further suggested that the recombination process which results in chiasma formation probably takes place in chromosomal regions lying outside the synaptonemal complex, rather than inside, between its 2 lateral elements."}
{"id": "PMID:479322", "title": "Survival of cells implanted in the embryonic chick limb bud: a difference between normal and malignant rat brain cells.", "content": "Cells from normal rat brain tissue did not survive and few cells could be found 1 d after grafting. In contrast, cells from a glioma and a carcinogen-treated rat brain survived well and many mitoses were observed. These malignant cells also invaded the limb. The behaviour of normal and malignant cells was followed at shorter times after grafting and some invasion by the normal cells was detected. The first signs of degeneration of normal cells were apparent around 7 h after grafting, and after this the grafts progressively deteriorated. These results support the ideal that the ability of cells to survive and grow in embryonic tissues is a characteristic of malignant cells. The findings are discussed in relation to mechanisms of tumour formation.", "contents": "Survival of cells implanted in the embryonic chick limb bud: a difference between normal and malignant rat brain cells. Cells from normal rat brain tissue did not survive and few cells could be found 1 d after grafting. In contrast, cells from a glioma and a carcinogen-treated rat brain survived well and many mitoses were observed. These malignant cells also invaded the limb. The behaviour of normal and malignant cells was followed at shorter times after grafting and some invasion by the normal cells was detected. The first signs of degeneration of normal cells were apparent around 7 h after grafting, and after this the grafts progressively deteriorated. These results support the ideal that the ability of cells to survive and grow in embryonic tissues is a characteristic of malignant cells. The findings are discussed in relation to mechanisms of tumour formation."}
{"id": "PMID:479323", "title": "Motility of the Limulus blood cell.", "content": "The sole cell type (the amoebocyte) found in the coelomic fluid of the horseshoe crab, Limulus polyphemus can be stimulated to become motile by extravasation or trauma. Motility was studied using time-lapse microcinematography and direct microscopic examination of cells in tissue culture and in gill leaflets isolated from young animals. Phase-contrast and Nomarski differential-interference contrast optics were employed. Both in culture and in the gills, motile cells showed 2 interconvertible morphological types: the contracted cell, which was compact and rounded and had a relatively small area of contact with the substratum, and a flattened from with a larger area of contact. In both morphological types, motility involved the protrusion of hyaline pseudopods followed by flow of granular endoplasm forward in the pseudoplod. Cellular motility in vivo (in the gill leaflet) was morphologically identical to that displayed in tissue culture. In culture, motility was unaffected by the nature of the substratum: cells were indistinguishable on fluid (paraffin oil) or solid (glass) substrata or on hydrophobic (paraffin oil, siliconized glass) or hydrophilic (clean glass) surfaces. Cells migrated and spread on agar surfaces. Cell motility was unaffected by high concentrations (100 micrograms/ml) of the microtubule-depolymerizing agent colcemid and was abolished by cytochalasin B at 1 microgram/ml.", "contents": "Motility of the Limulus blood cell. The sole cell type (the amoebocyte) found in the coelomic fluid of the horseshoe crab, Limulus polyphemus can be stimulated to become motile by extravasation or trauma. Motility was studied using time-lapse microcinematography and direct microscopic examination of cells in tissue culture and in gill leaflets isolated from young animals. Phase-contrast and Nomarski differential-interference contrast optics were employed. Both in culture and in the gills, motile cells showed 2 interconvertible morphological types: the contracted cell, which was compact and rounded and had a relatively small area of contact with the substratum, and a flattened from with a larger area of contact. In both morphological types, motility involved the protrusion of hyaline pseudopods followed by flow of granular endoplasm forward in the pseudoplod. Cellular motility in vivo (in the gill leaflet) was morphologically identical to that displayed in tissue culture. In culture, motility was unaffected by the nature of the substratum: cells were indistinguishable on fluid (paraffin oil) or solid (glass) substrata or on hydrophobic (paraffin oil, siliconized glass) or hydrophilic (clean glass) surfaces. Cells migrated and spread on agar surfaces. Cell motility was unaffected by high concentrations (100 micrograms/ml) of the microtubule-depolymerizing agent colcemid and was abolished by cytochalasin B at 1 microgram/ml."}
{"id": "PMID:479324", "title": "Production of nerveless Hydra attenuata by hydroxyurea treatments.", "content": "Hydroxyurea was used to produce hydra with varying nerve cell densities including a new type of nerveless animal. Hydra attenuata were treated with 10(-2) M hydroxyurea. By 23 days after treatment, 2 populations of animals are in culture. Both have a decrease in nerve cells. The first is a normal-coloured feeding animal (HU-R) and is recovering while the second is a pale non-feeding animal (HU-P). HU-P animals resemble nerveless animals in their lack of behavioural responses but they contain about 2% nerve cells. Upon hand feeding, some HU-P animals will recover but most will produce nerveless buds. Neverless hydra produced by hydroxyurea resemble nerveless animals produced by other techniques, in their behavioural, morphological and developmental properties. Normal animals, as cultured and in regeneration experiments, show a compact tentacle number distribution pattern with a small variance. Nerveless animals show broad tentacle distribution patterns with increased means and variances. It is suggested that a normal tentacle number regulatory mechanism is lacking or diminished in nerveless animals. This defect is correlated with hypostomal circumference and with nerve cells.", "contents": "Production of nerveless Hydra attenuata by hydroxyurea treatments. Hydroxyurea was used to produce hydra with varying nerve cell densities including a new type of nerveless animal. Hydra attenuata were treated with 10(-2) M hydroxyurea. By 23 days after treatment, 2 populations of animals are in culture. Both have a decrease in nerve cells. The first is a normal-coloured feeding animal (HU-R) and is recovering while the second is a pale non-feeding animal (HU-P). HU-P animals resemble nerveless animals in their lack of behavioural responses but they contain about 2% nerve cells. Upon hand feeding, some HU-P animals will recover but most will produce nerveless buds. Neverless hydra produced by hydroxyurea resemble nerveless animals produced by other techniques, in their behavioural, morphological and developmental properties. Normal animals, as cultured and in regeneration experiments, show a compact tentacle number distribution pattern with a small variance. Nerveless animals show broad tentacle distribution patterns with increased means and variances. It is suggested that a normal tentacle number regulatory mechanism is lacking or diminished in nerveless animals. This defect is correlated with hypostomal circumference and with nerve cells."}
{"id": "PMID:479325", "title": "Kinetics of the regeneration of sea-urchin cilia. II. Regeneration of animalized cilia.", "content": "The kinetics of the regeneration of cilia of Arbacia punctulata animalized by treatment with trypsin have been examined. The cilia regenerate with biphasic kinetics: an initial linear phase which changes abruptly to a second, slower linear rate. Inhibition of protein synthesis with 10 microM emetine has little effect on the first phase but totally abolishes the second, suggesting that the first phase represents the utilization of a pre-existing precursor pool while the second reflects de novo protein synthesis. However, cilia will regenerate again following a second deciliation in the presence of emetine, to half the plateau value of the first regeneration, indicating that only a fraction of the pool of the limiting precursor can be assembled into the regenerating cilium. It is proposed that a residual pool of this precursor is required to maintain the assembly-disassembly equilibrium in favour of the assembled organelle.", "contents": "Kinetics of the regeneration of sea-urchin cilia. II. Regeneration of animalized cilia. The kinetics of the regeneration of cilia of Arbacia punctulata animalized by treatment with trypsin have been examined. The cilia regenerate with biphasic kinetics: an initial linear phase which changes abruptly to a second, slower linear rate. Inhibition of protein synthesis with 10 microM emetine has little effect on the first phase but totally abolishes the second, suggesting that the first phase represents the utilization of a pre-existing precursor pool while the second reflects de novo protein synthesis. However, cilia will regenerate again following a second deciliation in the presence of emetine, to half the plateau value of the first regeneration, indicating that only a fraction of the pool of the limiting precursor can be assembled into the regenerating cilium. It is proposed that a residual pool of this precursor is required to maintain the assembly-disassembly equilibrium in favour of the assembled organelle."}
{"id": "PMID:479326", "title": "Morphological alterations in the mitochondria of Amoeba proteus induced by uncoupling agents.", "content": "Ultrastructural changes in mitochondrial morphology were observed in amoebae exposed to the uncoupling agents dinitrophernol (DNP), pentachlorophenol (PCP), and m-chlorocarbonyl cyanide phenylhydrazone (CCCP). These alterations occurred with rapidity and were present before whole-cell activity changes could be detected. They included changes in profile shape and overall dimensions, matrix density changes, and alterations to the cristal membranes, so that distinction between control Type I and Type II conformations was eliminated and a form with intermediate characters generated. It is proposed that, in producing these altered forms, the uncoupling agents are acting by suppressing the control functional states of the mitochondria. At the end of the uncoupler treatment all alterations were shown to be reversible, i.e. in cells transferred back to normal culture medium, mitochondrial profiles identical to those in control cells were again evident with time. These effects on the mitochondria could be produced whether the uncoupler was introduced externally by total cell exposure, or to the inside of the cell by microinjection. The importance of precisely defining conditions for treatments and at disrupting cell activity was evident. Thus, an uncoupler, which is a weak acid, proved to be more effective in whole cell treatments if applied at a pH near to it pKa - so ensuring its penetration across the outer cell membrane.", "contents": "Morphological alterations in the mitochondria of Amoeba proteus induced by uncoupling agents. Ultrastructural changes in mitochondrial morphology were observed in amoebae exposed to the uncoupling agents dinitrophernol (DNP), pentachlorophenol (PCP), and m-chlorocarbonyl cyanide phenylhydrazone (CCCP). These alterations occurred with rapidity and were present before whole-cell activity changes could be detected. They included changes in profile shape and overall dimensions, matrix density changes, and alterations to the cristal membranes, so that distinction between control Type I and Type II conformations was eliminated and a form with intermediate characters generated. It is proposed that, in producing these altered forms, the uncoupling agents are acting by suppressing the control functional states of the mitochondria. At the end of the uncoupler treatment all alterations were shown to be reversible, i.e. in cells transferred back to normal culture medium, mitochondrial profiles identical to those in control cells were again evident with time. These effects on the mitochondria could be produced whether the uncoupler was introduced externally by total cell exposure, or to the inside of the cell by microinjection. The importance of precisely defining conditions for treatments and at disrupting cell activity was evident. Thus, an uncoupler, which is a weak acid, proved to be more effective in whole cell treatments if applied at a pH near to it pKa - so ensuring its penetration across the outer cell membrane."}
{"id": "PMID:479327", "title": "Mechanical tension produced by nerve cells in tissue culture.", "content": "Evidence is presented that (a) the growth cone of cultured neurons can exert mechanical tension, and (b) that the direction of advance of the growth cone is determined by the tension existing between it and the rest of the cell. (a) The evidence that growth cones can pull comes from a vectorial analysis of the outlines of individually isolated sensory neurons. The angles formed in these outgrowths are very close to those of tension-generated networks anchored at their free ends and these values are restored shortly after an experimental displacement. The relative mechanical tension on each segment of an outgrowth can be calculated by standard methods and is found to decrease at each branch point. It appears to be correlated with the diameter of the fibre so that thicker fibres maintain more tension than thinner ones. (b) The influence of tension on the direction of advance of the growth cone is shown by 2 kinds of experient. If a growing neurite is pulled to one side with a microelectrode then the direction of its advance is changed immediately according to the new stress. If the mechanical tension on the growth cone of a neurite is released by amputation or displacement the growth cone is found to have a high probability of branching shortly afterwards. The ability of the growth cone to exert tension is discussed in relation to evidence that microspikes have contractile properties and in terms of the distribution of microfilaments within the neurite. It is suggested that the exertion of tension by a growth cone could serve to guide the neurite along paths of high adhesivity both in vitro and in vivo.", "contents": "Mechanical tension produced by nerve cells in tissue culture. Evidence is presented that (a) the growth cone of cultured neurons can exert mechanical tension, and (b) that the direction of advance of the growth cone is determined by the tension existing between it and the rest of the cell. (a) The evidence that growth cones can pull comes from a vectorial analysis of the outlines of individually isolated sensory neurons. The angles formed in these outgrowths are very close to those of tension-generated networks anchored at their free ends and these values are restored shortly after an experimental displacement. The relative mechanical tension on each segment of an outgrowth can be calculated by standard methods and is found to decrease at each branch point. It appears to be correlated with the diameter of the fibre so that thicker fibres maintain more tension than thinner ones. (b) The influence of tension on the direction of advance of the growth cone is shown by 2 kinds of experient. If a growing neurite is pulled to one side with a microelectrode then the direction of its advance is changed immediately according to the new stress. If the mechanical tension on the growth cone of a neurite is released by amputation or displacement the growth cone is found to have a high probability of branching shortly afterwards. The ability of the growth cone to exert tension is discussed in relation to evidence that microspikes have contractile properties and in terms of the distribution of microfilaments within the neurite. It is suggested that the exertion of tension by a growth cone could serve to guide the neurite along paths of high adhesivity both in vitro and in vivo."}
{"id": "PMID:479328", "title": "Interpolation of microtubules into cortical arrays during cell elongation and differentiation in roots of Azolla pinnata.", "content": "Longitudinal sections of roots of Azolla pinnata R. Br. were prepared for electron microscopy so that cortical microtubules could be counted along the longitudinal walls in cell files in the endodermis, pericycle, and inner and outer cortex, and in sieve and xylem elements. With the exception of the xylem, where there are no transverse cell divisions, each file of cells commences with its initial cell and then possesses a zone of concomitant cell expansion and transverse cell division, followed, after completion of the divisions, by a zone of terminal cell differentiation. The cells augment their population of cortical microtubules as they elongate and divide, showing a net increase of up to 0.6 micron of polymerized microtubule length per min. Two main sub-processes were found: (i) When a longitudinal wall is first formed it is supplied with a higher number of microtubules per unit length of wall than it will have later, when it is being expanded. This initial quota becomes diluted as the second sub-process commences. (ii) The cells interpolate new microtubules at a rate which is characteristic of the cell, and, in the endodermis, of the face of the cell, while the cell elongates. Most cell types thus maintain a set density of cortical microtubules while they elongate and divide. Comparisons of endodermal cells in untreated controls, and roots that had been treated with colchicine, low temperature, or high pressure indicate that the initial quota of microtubules, and the later interpolations, and differentially sensitive to microtuble perturbations. Three types of behaviour, all related to changes in the cell walls, were noted as cortex, xylem and sieve element cells entered their respective phases of cell differentiation. The cortical cells expanded in all dimensions, and the interpolation of microtubules diminished or ceased. The sieve elements continued to elongate, and interpolated at a high rate, reaching unusually high densities of microtubules when the cell walls were being thickened. During this period a net increase of 2.0 micron of polymerized microtubule length per min was calculated. Thereafter interpolation ceased and the density of microtubules declined. The sample applied to developing xylem except that, because wall-thickening is localized rather than widespread, the rise and subsequent fall in the density of microtubules was less marked. The data are discussed in relation to the participation of microtubules in wall deposition and to the hypothesis that cortical microtubules arise in discrete zones along the edges of cells.", "contents": "Interpolation of microtubules into cortical arrays during cell elongation and differentiation in roots of Azolla pinnata. Longitudinal sections of roots of Azolla pinnata R. Br. were prepared for electron microscopy so that cortical microtubules could be counted along the longitudinal walls in cell files in the endodermis, pericycle, and inner and outer cortex, and in sieve and xylem elements. With the exception of the xylem, where there are no transverse cell divisions, each file of cells commences with its initial cell and then possesses a zone of concomitant cell expansion and transverse cell division, followed, after completion of the divisions, by a zone of terminal cell differentiation. The cells augment their population of cortical microtubules as they elongate and divide, showing a net increase of up to 0.6 micron of polymerized microtubule length per min. Two main sub-processes were found: (i) When a longitudinal wall is first formed it is supplied with a higher number of microtubules per unit length of wall than it will have later, when it is being expanded. This initial quota becomes diluted as the second sub-process commences. (ii) The cells interpolate new microtubules at a rate which is characteristic of the cell, and, in the endodermis, of the face of the cell, while the cell elongates. Most cell types thus maintain a set density of cortical microtubules while they elongate and divide. Comparisons of endodermal cells in untreated controls, and roots that had been treated with colchicine, low temperature, or high pressure indicate that the initial quota of microtubules, and the later interpolations, and differentially sensitive to microtuble perturbations. Three types of behaviour, all related to changes in the cell walls, were noted as cortex, xylem and sieve element cells entered their respective phases of cell differentiation. The cortical cells expanded in all dimensions, and the interpolation of microtubules diminished or ceased. The sieve elements continued to elongate, and interpolated at a high rate, reaching unusually high densities of microtubules when the cell walls were being thickened. During this period a net increase of 2.0 micron of polymerized microtubule length per min was calculated. Thereafter interpolation ceased and the density of microtubules declined. The sample applied to developing xylem except that, because wall-thickening is localized rather than widespread, the rise and subsequent fall in the density of microtubules was less marked. The data are discussed in relation to the participation of microtubules in wall deposition and to the hypothesis that cortical microtubules arise in discrete zones along the edges of cells."}
{"id": "PMID:479329", "title": "Fine structure of meiotic prophase chromosomes and modified synaptonemal complexes in diploid and triploid Rhoeo spathacea.", "content": "The synaptonemal complex (SC) in the diploid Rhoeo consists of 2 amorphous lateral elements, each about 46.0 nm thick, and one amorphous central element about 30.0 nm thick. The central region is about 115.0 nm wide. SC in the triploid have essentially the same dimensions as those of the diploid; both lateral (46.0 nm) and central (30.0 nm) elements are amorphous, and the central region is about 117.5 nm wide. The coil, observed in both diploid and triploid, is a modified short segment of SC with several twists at the end of a synapsed bivalent that is attached to the nuclear membrane. Serial sections in a diploid cell reveal that a coil extends inwards about 3.5 micron from the nuclear membrane and makes a complete turn at a distance of every 0.5 micron. There is a correlation between the modified ends of SC and terminal chiasmata in Rhoeo. The coils might have a positive role in the process of crossing over, or alternatively might be involved in ring formation by holding chromosome ends together while chiasmata are not involved. SC are present in chromocentres of both diploid and triploid. Chromocentres in diploid and triploid are indistinguishable, and appear to be formed from the aggregation of pericentromeric heterochromatin as a result of translocations which occured close to the centromeres. 3-dimensional hypothetical pachytene configuration of the diploid is presented.", "contents": "Fine structure of meiotic prophase chromosomes and modified synaptonemal complexes in diploid and triploid Rhoeo spathacea. The synaptonemal complex (SC) in the diploid Rhoeo consists of 2 amorphous lateral elements, each about 46.0 nm thick, and one amorphous central element about 30.0 nm thick. The central region is about 115.0 nm wide. SC in the triploid have essentially the same dimensions as those of the diploid; both lateral (46.0 nm) and central (30.0 nm) elements are amorphous, and the central region is about 117.5 nm wide. The coil, observed in both diploid and triploid, is a modified short segment of SC with several twists at the end of a synapsed bivalent that is attached to the nuclear membrane. Serial sections in a diploid cell reveal that a coil extends inwards about 3.5 micron from the nuclear membrane and makes a complete turn at a distance of every 0.5 micron. There is a correlation between the modified ends of SC and terminal chiasmata in Rhoeo. The coils might have a positive role in the process of crossing over, or alternatively might be involved in ring formation by holding chromosome ends together while chiasmata are not involved. SC are present in chromocentres of both diploid and triploid. Chromocentres in diploid and triploid are indistinguishable, and appear to be formed from the aggregation of pericentromeric heterochromatin as a result of translocations which occured close to the centromeres. 3-dimensional hypothetical pachytene configuration of the diploid is presented."}
{"id": "PMID:479330", "title": "Probing the free space within rat and chicken chromatin with active and passive probes.", "content": "The cavity systems within chicken erythrocyte nuclei and rat liver nuclei were compared using passive probes of radioactive glycogen and active probes of nuclease-armed-glycogen. The passive probe curves have a form that indicates that they are due to passive occupation of spaces and not due to the effects of a limiting membrane. The technique of probing nuclei with glycogen armed with a small enzyme is described. The chicken erythrocytes appeared to have 11-15-nm and 4-5-nm cacity systems similar to those we have previously reported in rat nuclei. Evidence is presented to show that the bridge DNA is enclosed within the 4-5 nm cavities in both rat liver and chicken erythrocyte nuclei. Minor differences between the rat liver and chicken erythrocyte nuclear cavity systems are noted in the region of 5-8 nm. A relatively mild procedure is described for preparing chicken erythrocyte nuclei.", "contents": "Probing the free space within rat and chicken chromatin with active and passive probes. The cavity systems within chicken erythrocyte nuclei and rat liver nuclei were compared using passive probes of radioactive glycogen and active probes of nuclease-armed-glycogen. The passive probe curves have a form that indicates that they are due to passive occupation of spaces and not due to the effects of a limiting membrane. The technique of probing nuclei with glycogen armed with a small enzyme is described. The chicken erythrocytes appeared to have 11-15-nm and 4-5-nm cacity systems similar to those we have previously reported in rat nuclei. Evidence is presented to show that the bridge DNA is enclosed within the 4-5 nm cavities in both rat liver and chicken erythrocyte nuclei. Minor differences between the rat liver and chicken erythrocyte nuclear cavity systems are noted in the region of 5-8 nm. A relatively mild procedure is described for preparing chicken erythrocyte nuclei."}
{"id": "PMID:479331", "title": "[Complex hand injuries. Therapeutic management (author's transl)].", "content": "Defined by severe injuries of several digital structures and by lesions of several axes, complex hand injuries raise numerous problems of management. The activity of the unit \"S.O.S. Hand-Reimplantation\" of the Boucicaut Hospital petmitted us to determine the various clinical and socio-professional aspects. In the first part of this article, a statistical study permitted us to define the classes, types and prognosis. In the second part, the repair of each digital structure is considered (skin, bone, joint, extensors, flexors, nerves, vessels) in the light of a complex hand injury. The role of the covering skin in the quality of the results seems very important. The long-term results, their socio-professional consequences, thus permit one to draw up a therapeutic management which aims to repair the maximum number of lesions during the first stage of the operation. The interest of microsurgical repairs of the vessels and nerves requires operation of these complex hand injuries as an emergency, which requires appropriate surgical facilities.", "contents": "[Complex hand injuries. Therapeutic management (author's transl)]. Defined by severe injuries of several digital structures and by lesions of several axes, complex hand injuries raise numerous problems of management. The activity of the unit \"S.O.S. Hand-Reimplantation\" of the Boucicaut Hospital petmitted us to determine the various clinical and socio-professional aspects. In the first part of this article, a statistical study permitted us to define the classes, types and prognosis. In the second part, the repair of each digital structure is considered (skin, bone, joint, extensors, flexors, nerves, vessels) in the light of a complex hand injury. The role of the covering skin in the quality of the results seems very important. The long-term results, their socio-professional consequences, thus permit one to draw up a therapeutic management which aims to repair the maximum number of lesions during the first stage of the operation. The interest of microsurgical repairs of the vessels and nerves requires operation of these complex hand injuries as an emergency, which requires appropriate surgical facilities."}
{"id": "PMID:479332", "title": "[Aneurysms of the superior mesenteric artery and of its branches. Report of three cases (author's transl)].", "content": "The authors report three cases of atheromatous aneurysm revealed by a complication (e.g. rupture, thrombosis, peripheral embolisation) and propose an etiological, physio-pathological, clinical and therapeutic study of aneurysms of the superior mesenteric artery. They emphasise the advantages of early diagnosis leading to arteriography, which alone permits appropriate surgical treatment.", "contents": "[Aneurysms of the superior mesenteric artery and of its branches. Report of three cases (author's transl)]. The authors report three cases of atheromatous aneurysm revealed by a complication (e.g. rupture, thrombosis, peripheral embolisation) and propose an etiological, physio-pathological, clinical and therapeutic study of aneurysms of the superior mesenteric artery. They emphasise the advantages of early diagnosis leading to arteriography, which alone permits appropriate surgical treatment."}
{"id": "PMID:479333", "title": "[Fascicular nailing of the femur and tibia. 14 years' experience. Report of 275 cases (author's transl)].", "content": "The use of the fascicular four-pin nail has been introduced to correct certain inadequacies revealed by the rigid nailing according to Kuntscher's method; made up of four tiny pins soldered at each end, it fits the curve of the bone; its elasticity allows a much greater resistance to repeated strain as compared to other nails; an experimental study which compares the stability of fresh femurs cut then nailed according to different methods, reveals a definite superiority for the fascicular nail. The clinical use for 14 years (275 nailings among which 92 cases werefresh closed fractures of the femur) confirms that such a method provides the advantage of nailing without reaming as well as the possibility of immediate weight bearing ambulation for most patients (75% in the cases of resh femur or tibia fractures).", "contents": "[Fascicular nailing of the femur and tibia. 14 years' experience. Report of 275 cases (author's transl)]. The use of the fascicular four-pin nail has been introduced to correct certain inadequacies revealed by the rigid nailing according to Kuntscher's method; made up of four tiny pins soldered at each end, it fits the curve of the bone; its elasticity allows a much greater resistance to repeated strain as compared to other nails; an experimental study which compares the stability of fresh femurs cut then nailed according to different methods, reveals a definite superiority for the fascicular nail. The clinical use for 14 years (275 nailings among which 92 cases werefresh closed fractures of the femur) confirms that such a method provides the advantage of nailing without reaming as well as the possibility of immediate weight bearing ambulation for most patients (75% in the cases of resh femur or tibia fractures)."}
{"id": "PMID:479334", "title": "[Pulmonary hydatidosis in the child (author's transl)].", "content": "In general, pulmonary hydatidosis supposes 22% of the cases treated in a general surgery unity and 4.9% correspond to infantile hydatidosis (98 cases). The authors establish an anatomico-pathological classification of the cyst and make a comparative study with adults. The authors then expound on the different types of complications after the differents treatments used to treat hydatid disease since 1952. Post-operatory morbidity is 4.7% and mortality of 0.9%. These percentages are related to the anatomico-pathological type of the cyst.", "contents": "[Pulmonary hydatidosis in the child (author's transl)]. In general, pulmonary hydatidosis supposes 22% of the cases treated in a general surgery unity and 4.9% correspond to infantile hydatidosis (98 cases). The authors establish an anatomico-pathological classification of the cyst and make a comparative study with adults. The authors then expound on the different types of complications after the differents treatments used to treat hydatid disease since 1952. Post-operatory morbidity is 4.7% and mortality of 0.9%. These percentages are related to the anatomico-pathological type of the cyst."}
{"id": "PMID:479335", "title": "[Idiopathic congenital dilatation of the common bile duct and pregnancy. Report of one case, reflexions and comments (author's transl)].", "content": "Congenital idiopathic dilatation of the common bile duct, called commonly and incorrectly congenital cyst of the common bile duct, is sometimes diagnosed during pregnancy. The latter may be the source of severe complications. The disease should thus be treated rapidly, even if the pregnancy is at a late stage. In a case of this type we carried out surgical treatment in two stages: firstly simple external drainage of the common bile duct and of the gall bladder and only after labour has been brought to term by Caesarian section, for breech presentation, was a cystojejunostomy carried out on a Y shaped jejunal loop.", "contents": "[Idiopathic congenital dilatation of the common bile duct and pregnancy. Report of one case, reflexions and comments (author's transl)]. Congenital idiopathic dilatation of the common bile duct, called commonly and incorrectly congenital cyst of the common bile duct, is sometimes diagnosed during pregnancy. The latter may be the source of severe complications. The disease should thus be treated rapidly, even if the pregnancy is at a late stage. In a case of this type we carried out surgical treatment in two stages: firstly simple external drainage of the common bile duct and of the gall bladder and only after labour has been brought to term by Caesarian section, for breech presentation, was a cystojejunostomy carried out on a Y shaped jejunal loop."}
{"id": "PMID:479336", "title": "[Experimental study of the permeability of the fallopian tubes in the rabbit after division of the isthmus and microsurgical anastomosis (author's transl)].", "content": "The poor results of traditional reconstructive surgery of the Fallopian tubes have led the authors to try the possibilities of microsurgery in tubal sterility. The experimental animal chosen was the rabbit owing to its similarities with the Fallopian tube in women. After division of the isthumus, the tube was repermeabilised with a 10.0 single nylon thread under the operating microscope. A series of 11 rabbits were then coupled one mont after the operation. The pregnancy rate was 55%. This figure was much better than those obtained in the literature by non-microsurgical technics. One may hope to improve the results further by less traumatic surgery, better asepsis, and better control of fertility.", "contents": "[Experimental study of the permeability of the fallopian tubes in the rabbit after division of the isthmus and microsurgical anastomosis (author's transl)]. The poor results of traditional reconstructive surgery of the Fallopian tubes have led the authors to try the possibilities of microsurgery in tubal sterility. The experimental animal chosen was the rabbit owing to its similarities with the Fallopian tube in women. After division of the isthumus, the tube was repermeabilised with a 10.0 single nylon thread under the operating microscope. A series of 11 rabbits were then coupled one mont after the operation. The pregnancy rate was 55%. This figure was much better than those obtained in the literature by non-microsurgical technics. One may hope to improve the results further by less traumatic surgery, better asepsis, and better control of fertility."}
{"id": "PMID:479337", "title": "The quantitative analysis of 1-alpha-acetylmethadol and its principal metabolites in biological specimens by gas chromatography-chemical ionization-multiple ion monitoring mass spectrometry.", "content": "1-alpha-acetylmethadol (LAAM) is a new drug under development for the treatment of heroin dependence. A new analytical  method applicable to the accurate biodispositional study of the drug and its metabolities is described and critically discussed in this report. The procedure involves sample preparation and direct organic solvent extraction using eta-butyl chloride, amide derivatization by molecular rearrangement, and gas chromatography-chemical ionization mass spectrometry-selected ion monitoring, with methane as the carrier and ammonia as reagent gases. Deuterated (d3 stable isotopes of LAAM and its metabolites are used as internal standards. The method is free from qualitative interferences and has quantitative sensitivity to 5 ng/ml for 2.0 ml samples with 10-15% accuracy and precision in the range 5-100 ng/ml; and 2-5% at concentrations up to 750 ng/ml. Specimens of plasma, whole blood, urine, bile, brain, liver, and other visceral samples have been successfully analyzed, as well as in vitro preparations such as hepatic microsomes. By appropriate data processing, the method lends itself to routine analysis and high volume work; even manually the method is capable of at least 50 samples per week. A simplified procedure for the analysis of LAAM and its metabolites in urine only is also presented and discuet up and use the methods.", "contents": "The quantitative analysis of 1-alpha-acetylmethadol and its principal metabolites in biological specimens by gas chromatography-chemical ionization-multiple ion monitoring mass spectrometry. 1-alpha-acetylmethadol (LAAM) is a new drug under development for the treatment of heroin dependence. A new analytical  method applicable to the accurate biodispositional study of the drug and its metabolities is described and critically discussed in this report. The procedure involves sample preparation and direct organic solvent extraction using eta-butyl chloride, amide derivatization by molecular rearrangement, and gas chromatography-chemical ionization mass spectrometry-selected ion monitoring, with methane as the carrier and ammonia as reagent gases. Deuterated (d3 stable isotopes of LAAM and its metabolites are used as internal standards. The method is free from qualitative interferences and has quantitative sensitivity to 5 ng/ml for 2.0 ml samples with 10-15% accuracy and precision in the range 5-100 ng/ml; and 2-5% at concentrations up to 750 ng/ml. Specimens of plasma, whole blood, urine, bile, brain, liver, and other visceral samples have been successfully analyzed, as well as in vitro preparations such as hepatic microsomes. By appropriate data processing, the method lends itself to routine analysis and high volume work; even manually the method is capable of at least 50 samples per week. A simplified procedure for the analysis of LAAM and its metabolites in urine only is also presented and discuet up and use the methods."}
{"id": "PMID:479338", "title": "Determination of paraquat in marijuana by reversed-phase paired-ion high performance liquid chromatography.", "content": "A sensitive and selective method is described for the quantitative determination of paraquat in marijuana. Paraquat is extracted from finely ground plant material with hydrochloric acid with sonification, and the resulting acidic solution is extracted with chloroform:isopropanol (9:1) and evaporated to dryness. The residue is reconstituted with aqueous phosphate buffer pH 7.0; the solution is passed through a C-18 SEP-PAK TM and is analyzed with high performance liquid chromatography, using a reversed-phase column and an \"ion pairing\" reagent in the mobile phase. The recovery of paraquat in laboratory-spiked material varied from 90-97%. Results obtained with confiscated, field-sprayed marijuana by the procedure described were in excellent agreement with those obtained with a well-established ultraviolet procedure. The calculated limit of detection with this method is 2 ng of paraquat.", "contents": "Determination of paraquat in marijuana by reversed-phase paired-ion high performance liquid chromatography. A sensitive and selective method is described for the quantitative determination of paraquat in marijuana. Paraquat is extracted from finely ground plant material with hydrochloric acid with sonification, and the resulting acidic solution is extracted with chloroform:isopropanol (9:1) and evaporated to dryness. The residue is reconstituted with aqueous phosphate buffer pH 7.0; the solution is passed through a C-18 SEP-PAK TM and is analyzed with high performance liquid chromatography, using a reversed-phase column and an \"ion pairing\" reagent in the mobile phase. The recovery of paraquat in laboratory-spiked material varied from 90-97%. Results obtained with confiscated, field-sprayed marijuana by the procedure described were in excellent agreement with those obtained with a well-established ultraviolet procedure. The calculated limit of detection with this method is 2 ng of paraquat."}
{"id": "PMID:479339", "title": "Trenbolone acetate and trenbolone: trace analysis in animal chow, wastewater and human urine by high pressure liquid chromatography and electron capture gas chromatography.", "content": "Analytical methodology is described for determining residues of the synthetic anabolic steroid trenbolone acetate (TBA) and its hydrolysis product trenbolone (TBOH) in admixture in animal chow, human urine, and wastewater. Benzene extracts of the substrates are subjected to liquid-liquid partitioning, further cleanup on a column of silica gel, and direct analysis by high pressure liquid chromotography or derivatization with pentafluoropropionic anhydride and analysis by electron capture gas chromatography. Satisfactory recoveries were obtained with both compounds from all three substrates. Residue levels of TBA and TBOH as low as 0.32 and 0.04 ppm, respectively, could be detected in chow; about 0.6 ppb of each compound could be detected in urine and wastewater. Thin layer chromatographic behavior of the two compounds in 7 solvent systems and other ancillary analytical data are also presented.", "contents": "Trenbolone acetate and trenbolone: trace analysis in animal chow, wastewater and human urine by high pressure liquid chromatography and electron capture gas chromatography. Analytical methodology is described for determining residues of the synthetic anabolic steroid trenbolone acetate (TBA) and its hydrolysis product trenbolone (TBOH) in admixture in animal chow, human urine, and wastewater. Benzene extracts of the substrates are subjected to liquid-liquid partitioning, further cleanup on a column of silica gel, and direct analysis by high pressure liquid chromotography or derivatization with pentafluoropropionic anhydride and analysis by electron capture gas chromatography. Satisfactory recoveries were obtained with both compounds from all three substrates. Residue levels of TBA and TBOH as low as 0.32 and 0.04 ppm, respectively, could be detected in chow; about 0.6 ppb of each compound could be detected in urine and wastewater. Thin layer chromatographic behavior of the two compounds in 7 solvent systems and other ancillary analytical data are also presented."}
{"id": "PMID:479340", "title": "Derivative formation in the chromatographic analysis of food additives.", "content": "The use of derivatization in the analysis of food additivies by chromatographic means has been reviewed. The large majority of these derivatization techniques are employed to facilitate the gas-liquid chromatographic quantitation of various food additives. Silylation, alkylation, acylation, as well as transesterification, saponification, and chemical decomposition techniques are discussed. The review includes procedures for the analysis of emulsifiers and stabilizers, artificial sweeteners, antioxidants, preservatives, gums, and waxes, and draws in part, from work conducted in the authors' laboratory.", "contents": "Derivative formation in the chromatographic analysis of food additives. The use of derivatization in the analysis of food additivies by chromatographic means has been reviewed. The large majority of these derivatization techniques are employed to facilitate the gas-liquid chromatographic quantitation of various food additives. Silylation, alkylation, acylation, as well as transesterification, saponification, and chemical decomposition techniques are discussed. The review includes procedures for the analysis of emulsifiers and stabilizers, artificial sweeteners, antioxidants, preservatives, gums, and waxes, and draws in part, from work conducted in the authors' laboratory."}
{"id": "PMID:479341", "title": "Metabolic clearance rates of isohormones of human growth hormone in man.", "content": "The charge isomers of human GH (/GH), isohormones C, D, and E, which result from limited proteolytic cleavage exhibit enhanced biological activity in the rat and in man compared to the intact polypeptide and most abundant form, isohormone B. The mechanism leading to enhanced activity is unknown. To examine the possibility that different in vivo metabolism may represent one such mechanism, the MCRs of radioiodinated hGH-B, -C, and -D were measured in seven normal adults. Significant differences in metabolic clearance were found between isohormones (hGH-B, 108.4; hGH-C, 67.9; hGH-D, 55.7 liters/m2-day). It is concluded that in man, the enzymatically cleaved hGH isohormones are cleared more slowly than hGH-B. Slower metabolism may be a factor responsible for the enhanced biological activity of proteolytically cleaved hGH isohormones.", "contents": "Metabolic clearance rates of isohormones of human growth hormone in man. The charge isomers of human GH (/GH), isohormones C, D, and E, which result from limited proteolytic cleavage exhibit enhanced biological activity in the rat and in man compared to the intact polypeptide and most abundant form, isohormone B. The mechanism leading to enhanced activity is unknown. To examine the possibility that different in vivo metabolism may represent one such mechanism, the MCRs of radioiodinated hGH-B, -C, and -D were measured in seven normal adults. Significant differences in metabolic clearance were found between isohormones (hGH-B, 108.4; hGH-C, 67.9; hGH-D, 55.7 liters/m2-day). It is concluded that in man, the enzymatically cleaved hGH isohormones are cleared more slowly than hGH-B. Slower metabolism may be a factor responsible for the enhanced biological activity of proteolytically cleaved hGH isohormones."}
{"id": "PMID:479342", "title": "Failure of estrogen-induced discharge of luteinizing hormone in lactating women.", "content": "Pituitary-ovarian relationships were studied in seven lactating women by measuring the basal plasma concentrations of pituitary and ovarian hormones and their responses to an estrogen provocation test at 7, 30, and 100 days after delivery. The results were compared to a similar group of seven women who did not breast feed. The first ovulation occurred in five of the nonlactating women beteen 43-87 days after delivery, as judged by the urinary excretion of total estrogen and pregnanediol. In all lactating women, ovarian cyclicity was suppressed for at least 150 days after delivery or until weaning. The basal concentration of PRL in lactating women was significantly higher than in the nonlactating women at all three times measured. At 30 and 100 days, the concentration of estradiol was significantly lower in the lactating women, although the basal concentrations of FSH were similar in the two groups. After an injection of 1 mg estradiol benzoate, the concentrations of FSH and LH in plasma were suppressed to a greater extent in lactating than in nonlactating women. In addition, fewer of the lactating group (one of seven and none of seven at 30 and 100 days, respectively) than the nonlactating group (two of seven and five of seven) subsequently showed a rise in the concentration of LH 58--96 h after the estrogen injection (positive feedback). These results suggest that during lactation the hypothalamic-pituitary system is more sensitive to the negative feedback and relatively insensitive to the positive feedback of estrogen.", "contents": "Failure of estrogen-induced discharge of luteinizing hormone in lactating women. Pituitary-ovarian relationships were studied in seven lactating women by measuring the basal plasma concentrations of pituitary and ovarian hormones and their responses to an estrogen provocation test at 7, 30, and 100 days after delivery. The results were compared to a similar group of seven women who did not breast feed. The first ovulation occurred in five of the nonlactating women beteen 43-87 days after delivery, as judged by the urinary excretion of total estrogen and pregnanediol. In all lactating women, ovarian cyclicity was suppressed for at least 150 days after delivery or until weaning. The basal concentration of PRL in lactating women was significantly higher than in the nonlactating women at all three times measured. At 30 and 100 days, the concentration of estradiol was significantly lower in the lactating women, although the basal concentrations of FSH were similar in the two groups. After an injection of 1 mg estradiol benzoate, the concentrations of FSH and LH in plasma were suppressed to a greater extent in lactating than in nonlactating women. In addition, fewer of the lactating group (one of seven and none of seven at 30 and 100 days, respectively) than the nonlactating group (two of seven and five of seven) subsequently showed a rise in the concentration of LH 58--96 h after the estrogen injection (positive feedback). These results suggest that during lactation the hypothalamic-pituitary system is more sensitive to the negative feedback and relatively insensitive to the positive feedback of estrogen."}
{"id": "PMID:479343", "title": "Roles of estradiol and progesterone in eiliciting the midcycle luteinizing hormone and follicle-stimulating hormone surges.", "content": "The positive feedback effects of estradiol (E2) and progesterone (P) on LH and FSH release were studied under novel experimental conditions in three women of reproductive age who had undergone oophorectomy and received uninterupted E2 replacement by subdermal implants. Basal serum E2 levels were in the midfollicular phase range, while LH and FSH levels were normal or slightly elevated. Each volunteer underwent seven experiments at 2- to 4-week intervals, receiving im injections of increasing amounts of estradiol benzoate (E2B) alone and in combination with P. The time and dose of P (administered via P-impregnated polysiloxane intravaginal rings) were varied. In two of the seven experiments, P was given without E2B injections. In all three subjects, increasing serum E2 levels mimicking the preovulatory E2 peak were followed by a surge of LH but not of FSH. However, when serum P levels rose after an increase in serum E2 concentrations had occurred, the LH surge occurred earlier and was accompanied by an FSH peak. When serum P levels rose before serum E2 concentrations had risen or when P levels increased without a rise in serum E2, neither a serum LH nor FSH peak was observed. When administered concomitantly, E2B and P suppressed FSH but not LH levels, while P alone did not affect serum LH or FSH concentrations. These data indicate that an acute rise in serum E2 is a necessary condition for the midcycle LH and FSH surges, that P facilitates or blocks the positive feedback response of gonadotropin release in a time-dependent manner, and that P is required for the preovulatory FSH peak.", "contents": "Roles of estradiol and progesterone in eiliciting the midcycle luteinizing hormone and follicle-stimulating hormone surges. The positive feedback effects of estradiol (E2) and progesterone (P) on LH and FSH release were studied under novel experimental conditions in three women of reproductive age who had undergone oophorectomy and received uninterupted E2 replacement by subdermal implants. Basal serum E2 levels were in the midfollicular phase range, while LH and FSH levels were normal or slightly elevated. Each volunteer underwent seven experiments at 2- to 4-week intervals, receiving im injections of increasing amounts of estradiol benzoate (E2B) alone and in combination with P. The time and dose of P (administered via P-impregnated polysiloxane intravaginal rings) were varied. In two of the seven experiments, P was given without E2B injections. In all three subjects, increasing serum E2 levels mimicking the preovulatory E2 peak were followed by a surge of LH but not of FSH. However, when serum P levels rose after an increase in serum E2 concentrations had occurred, the LH surge occurred earlier and was accompanied by an FSH peak. When serum P levels rose before serum E2 concentrations had risen or when P levels increased without a rise in serum E2, neither a serum LH nor FSH peak was observed. When administered concomitantly, E2B and P suppressed FSH but not LH levels, while P alone did not affect serum LH or FSH concentrations. These data indicate that an acute rise in serum E2 is a necessary condition for the midcycle LH and FSH surges, that P facilitates or blocks the positive feedback response of gonadotropin release in a time-dependent manner, and that P is required for the preovulatory FSH peak."}
{"id": "PMID:479346", "title": "Aluminum uptake by the parathyroid glands.", "content": "Aluminum-containing drugs are used extensively to bind dietary phosphate and as antacids, but little is known about toxicity and tissue uptake of ingested aluminum. Aluminum concentrations were measured by neutron activation analysis in tissues taken from hyperparathyroid and normal human subjects and from rats. The parathyroid glands contained significantly more aluminum per unit mass than did thyroid or cervical muscle. The concentration of aluminum in the parathyroids appears to be linearly related to dietary aluminum intake.", "contents": "Aluminum uptake by the parathyroid glands. Aluminum-containing drugs are used extensively to bind dietary phosphate and as antacids, but little is known about toxicity and tissue uptake of ingested aluminum. Aluminum concentrations were measured by neutron activation analysis in tissues taken from hyperparathyroid and normal human subjects and from rats. The parathyroid glands contained significantly more aluminum per unit mass than did thyroid or cervical muscle. The concentration of aluminum in the parathyroids appears to be linearly related to dietary aluminum intake."}
{"id": "PMID:479347", "title": "A solid phase, sandwich-type radioimmunoassay for antithyroglobulin: elimination of false positive results and semiquantitative measurement of antithyroglobulin in the presence of elevated thyroglobulin.", "content": "Thyroid disorders can be associated with elevated concentrations of serum antithyroglobulin antibodies (anti-Tg) and/or thyroglobulin (Tg), but none of the available anti-Tg assays deals with anti-Tg measurements in the presence of abnormally high Tg levels. The competitive binding radioassay produces falsely elevated values for anti-Tg if serum Tg is elevated and either falsely elevated or depressed values if both Tg and anti-Tg are abnormally high. The falsely elevated anti-Tg values can be identified by measuring the formation of Tg[125I]anti-Tg complexes in the supernatant of the anti-Tg assay (supernatant assay). For screening purposes, we modified the original anti-Tg RIA into a solid phase, sandwich-type RIA. Anti-Tg in serum or standard is first bound to plastic cups coated with Tg and then quantitated by binding of [125I]Tg. This assay has a detection limit of 2 U/ml serum, intra- and interassay coefficients of variation of 9--15%, and a normal range of less than 2 U/ml. When sera with normal Tg concentrations were analyzed, the results for anti-Tg obtained by the competitive binding RIA and the new sandwich RIA were comparable as far as positives and negatives were concerned, and the numerical values for positive sera correlated moderately well (r = 0.79); the sandwich assay, in general, gave lower values for anti-Tg. The major advantages of the sandwich anti-Tg RIA are the elimination of false positive results and its applicability to sera containing high levels of both Tg and anti-Tg. In the latter case, the results indicate the level of free anti-Tg present, as opposed to antibody present in the form of Tg-anti-Tg complexes.", "contents": "A solid phase, sandwich-type radioimmunoassay for antithyroglobulin: elimination of false positive results and semiquantitative measurement of antithyroglobulin in the presence of elevated thyroglobulin. Thyroid disorders can be associated with elevated concentrations of serum antithyroglobulin antibodies (anti-Tg) and/or thyroglobulin (Tg), but none of the available anti-Tg assays deals with anti-Tg measurements in the presence of abnormally high Tg levels. The competitive binding radioassay produces falsely elevated values for anti-Tg if serum Tg is elevated and either falsely elevated or depressed values if both Tg and anti-Tg are abnormally high. The falsely elevated anti-Tg values can be identified by measuring the formation of Tg[125I]anti-Tg complexes in the supernatant of the anti-Tg assay (supernatant assay). For screening purposes, we modified the original anti-Tg RIA into a solid phase, sandwich-type RIA. Anti-Tg in serum or standard is first bound to plastic cups coated with Tg and then quantitated by binding of [125I]Tg. This assay has a detection limit of 2 U/ml serum, intra- and interassay coefficients of variation of 9--15%, and a normal range of less than 2 U/ml. When sera with normal Tg concentrations were analyzed, the results for anti-Tg obtained by the competitive binding RIA and the new sandwich RIA were comparable as far as positives and negatives were concerned, and the numerical values for positive sera correlated moderately well (r = 0.79); the sandwich assay, in general, gave lower values for anti-Tg. The major advantages of the sandwich anti-Tg RIA are the elimination of false positive results and its applicability to sera containing high levels of both Tg and anti-Tg. In the latter case, the results indicate the level of free anti-Tg present, as opposed to antibody present in the form of Tg-anti-Tg complexes."}
{"id": "PMID:479348", "title": "Plasma vasopressin and human neurophysins in physiological and pathological states associated with changes in vasopressin secretion.", "content": "Using sensitive specific RIAs for vasopressin (AVP) and the two major human neurophysins, the relationship between AVP and the individual human neurophysins was investigated in man by measuring changes in plasma concentrations in physiological and pathological states known to be associated with changes in AVP secretion. Dehydration, water loading, and hemorrhage produced small but significant changes in plasma AVP concentrations without changes in the individual human neurophysins. In response to the stimulus of cigarette smoke inhalation, large parallel changes in plasma AVP and human neurophysin I (HNPI) levels were seen without change in plasma human neurophysin II (HNPII) levels. In the pathological states of diabetes insipidus and the syndrome of inappropriate antidiuretic hormone secretion,the observations more strongly supported a specific association between AVP and NHPI. In eight patients with central diabetes insipidus, plasma AVP and HNPI levels were low or undetectable, while plasma HNPII levels were normal. There was a clear distinction of both plasma AVP and HNPI levels in patients with central diabetes insipidus and those in patients whti nephrogenic diabetes insipidus. In 14 patients with the syndrome of inappropriate antidiuretic hormone secretion due to causes other than ectopic AVP production from tumors, plasma AVP and HNPI levels were elevated or normal, while plasma HNPII levels were normal. There was a highly significant positive correlation (r = 0.99) between plasma AVP and HNPI levels in these patients, with a 1:1 molar ratio. These data suggest that the secretion of AVP and HNPI in man are functionally related, while the secretion of HNPII is independent of AVP secretion.", "contents": "Plasma vasopressin and human neurophysins in physiological and pathological states associated with changes in vasopressin secretion. Using sensitive specific RIAs for vasopressin (AVP) and the two major human neurophysins, the relationship between AVP and the individual human neurophysins was investigated in man by measuring changes in plasma concentrations in physiological and pathological states known to be associated with changes in AVP secretion. Dehydration, water loading, and hemorrhage produced small but significant changes in plasma AVP concentrations without changes in the individual human neurophysins. In response to the stimulus of cigarette smoke inhalation, large parallel changes in plasma AVP and human neurophysin I (HNPI) levels were seen without change in plasma human neurophysin II (HNPII) levels. In the pathological states of diabetes insipidus and the syndrome of inappropriate antidiuretic hormone secretion,the observations more strongly supported a specific association between AVP and NHPI. In eight patients with central diabetes insipidus, plasma AVP and HNPI levels were low or undetectable, while plasma HNPII levels were normal. There was a clear distinction of both plasma AVP and HNPI levels in patients with central diabetes insipidus and those in patients whti nephrogenic diabetes insipidus. In 14 patients with the syndrome of inappropriate antidiuretic hormone secretion due to causes other than ectopic AVP production from tumors, plasma AVP and HNPI levels were elevated or normal, while plasma HNPII levels were normal. There was a highly significant positive correlation (r = 0.99) between plasma AVP and HNPI levels in these patients, with a 1:1 molar ratio. These data suggest that the secretion of AVP and HNPI in man are functionally related, while the secretion of HNPII is independent of AVP secretion."}
{"id": "PMID:479349", "title": "Renal clearance of estriol and its conjugates in normal and abnormal pregnancies.", "content": "Renal clearance of estriol (E3), E3-3-sulfate (E3S), E3-16-glucuronide (E316G), E3-3-glucuronide (E33G), and E3-3-sulfate-16-glucuronide (E3SG) has been measured in 13 normal and 17 abnormal pregnancies between the 33rd and 40th weeks of gestation. The methodology involved a chromatographic separation on a celite column in the presence of tritiated tracers, enzymic hydrolysis of the conjugates, and measurement of the E3 moiety by a specific RIA. Preeclampsia (eight patients) was characterized by a significant decrease of renal clearance for all conjugates except E33G. In contrast to that pattern, a patient with cholestatic icterus had normal clearances except for E33G, which was reduced to less than 15% of its normal mean value. Administration of ampicillin to a pregnant woman induced an important decrease of all E3 clearances, especially for E33G which decreased to nearly 1% of its initial value; normal clearances were resumed within a week after the end of the treatment. In a small number of complicated pregnancies, E3 clearances were normal. A significant correlation between the renal clearance of E316G and that of urea and creatinine has been demonstrated.", "contents": "Renal clearance of estriol and its conjugates in normal and abnormal pregnancies. Renal clearance of estriol (E3), E3-3-sulfate (E3S), E3-16-glucuronide (E316G), E3-3-glucuronide (E33G), and E3-3-sulfate-16-glucuronide (E3SG) has been measured in 13 normal and 17 abnormal pregnancies between the 33rd and 40th weeks of gestation. The methodology involved a chromatographic separation on a celite column in the presence of tritiated tracers, enzymic hydrolysis of the conjugates, and measurement of the E3 moiety by a specific RIA. Preeclampsia (eight patients) was characterized by a significant decrease of renal clearance for all conjugates except E33G. In contrast to that pattern, a patient with cholestatic icterus had normal clearances except for E33G, which was reduced to less than 15% of its normal mean value. Administration of ampicillin to a pregnant woman induced an important decrease of all E3 clearances, especially for E33G which decreased to nearly 1% of its initial value; normal clearances were resumed within a week after the end of the treatment. In a small number of complicated pregnancies, E3 clearances were normal. A significant correlation between the renal clearance of E316G and that of urea and creatinine has been demonstrated."}
{"id": "PMID:479350", "title": "The effect of angiotensin II and saralasin on 18-hydroxy-11-deoxycorticosterone production by isolated human adrenal glomerulosa cells.", "content": "To assess the role of angiotensin II (AII) in regulating 18-hydroxy-11-deoxycorticosterone (18-OHDOC) secretion in man, isolated human adrenal glomerulosa cells were incubated with AII and/or its competitive antagonist, saralasin. AII 2.4 X 10(-8) M) elicited an 80% increase in 18-OHDOC levels as well as similar increases in aldosterone, 18-hydroxycorticosterone, and corticosterone (P less than 0.01). Saralasin (10(-8) M) caused a partial but significant inhibition of AII-stimulated 18-OHDOC production, while 10(-6) M saralasin blocked AII-stimulated steroidogenesis completely. In addition, both concentrations of saralasin caused 10--30% decrements in basal steroid levels. The direct AII effect on 18-OHDOC secretion and the antagonistic effect of saralasin on both exogenous and endogenous AII-stimulated steroidogenesis, documented in these experiments, indicate that the increase in 18-OHDOC levels after sodium restriction reported in man is probably mediated by the renin-angiotensin system. Furthermore, because high concentrations of saralasin did not increase aldosterone secretion, the partial agonist properties of saralasin in vivo in man may not be due to a direct effect on the glomerulosa cell.", "contents": "The effect of angiotensin II and saralasin on 18-hydroxy-11-deoxycorticosterone production by isolated human adrenal glomerulosa cells. To assess the role of angiotensin II (AII) in regulating 18-hydroxy-11-deoxycorticosterone (18-OHDOC) secretion in man, isolated human adrenal glomerulosa cells were incubated with AII and/or its competitive antagonist, saralasin. AII 2.4 X 10(-8) M) elicited an 80% increase in 18-OHDOC levels as well as similar increases in aldosterone, 18-hydroxycorticosterone, and corticosterone (P less than 0.01). Saralasin (10(-8) M) caused a partial but significant inhibition of AII-stimulated 18-OHDOC production, while 10(-6) M saralasin blocked AII-stimulated steroidogenesis completely. In addition, both concentrations of saralasin caused 10--30% decrements in basal steroid levels. The direct AII effect on 18-OHDOC secretion and the antagonistic effect of saralasin on both exogenous and endogenous AII-stimulated steroidogenesis, documented in these experiments, indicate that the increase in 18-OHDOC levels after sodium restriction reported in man is probably mediated by the renin-angiotensin system. Furthermore, because high concentrations of saralasin did not increase aldosterone secretion, the partial agonist properties of saralasin in vivo in man may not be due to a direct effect on the glomerulosa cell."}
{"id": "PMID:479351", "title": "Metabolic effects of glucose, mannose, galactose, and fructose in man.", "content": "The effects of various hexoses upon immunoreactive insulin (IRI) secretion, glucose disposal, and gastric inhibitory polypeptide (GIP) release have been compared in 10 normal nonobese men. Rapid iv infusion (0.5 g/kg in 3 min) of D-mannose resulted in significant ITI release, the peak levels approaching those after D-glucose infusion. D-Galactose, however, was ineffective. The 60-min urine excretions of mannose, galactose, and glucose were 35 +/- 7%, 16 +/- 4%, and 5.5 +/- 0.7% (mean +/- SEM) of the administered dose, respectively. All subjects also received 50 g oral glucose, mannose, galactose, and fructose on different days, each followed by an iv glucose infusion 30 min later. The ingestion of glucose or galactose resulted in a similar increment of GIP (P less than 0.01), followed by a similar increment in the IRI response to iv glucose. Furthermore, the glucose disposal rate increased 2.5-fold compared to that after iv glucose alone (P less than 0.001). However, oral msnnose or oral fructose caused no significant GIP release, yet the IRI response to a subsequent iv glucose load was moderately augmented after oral mannose or oral fructose when compared to iv glucose alone. In addition, there was a similar enhancement of glucose disposal of the iv glucose load after both oral mannose and oral fructose (P less than 0.01). From these studies we conclude that 1) galactose does not elicit IRI secretion per se, yet, like glucose, potentiates GIP and IRI secretion; 2) mannose, despite weak transport across gut or kidney, evokes significant betacytotropic effects; and 3) mannose- and fructose-induced enhancement of glucose disposal might be mediated by a factor(s) other than GIP.", "contents": "Metabolic effects of glucose, mannose, galactose, and fructose in man. The effects of various hexoses upon immunoreactive insulin (IRI) secretion, glucose disposal, and gastric inhibitory polypeptide (GIP) release have been compared in 10 normal nonobese men. Rapid iv infusion (0.5 g/kg in 3 min) of D-mannose resulted in significant ITI release, the peak levels approaching those after D-glucose infusion. D-Galactose, however, was ineffective. The 60-min urine excretions of mannose, galactose, and glucose were 35 +/- 7%, 16 +/- 4%, and 5.5 +/- 0.7% (mean +/- SEM) of the administered dose, respectively. All subjects also received 50 g oral glucose, mannose, galactose, and fructose on different days, each followed by an iv glucose infusion 30 min later. The ingestion of glucose or galactose resulted in a similar increment of GIP (P less than 0.01), followed by a similar increment in the IRI response to iv glucose. Furthermore, the glucose disposal rate increased 2.5-fold compared to that after iv glucose alone (P less than 0.001). However, oral msnnose or oral fructose caused no significant GIP release, yet the IRI response to a subsequent iv glucose load was moderately augmented after oral mannose or oral fructose when compared to iv glucose alone. In addition, there was a similar enhancement of glucose disposal of the iv glucose load after both oral mannose and oral fructose (P less than 0.01). From these studies we conclude that 1) galactose does not elicit IRI secretion per se, yet, like glucose, potentiates GIP and IRI secretion; 2) mannose, despite weak transport across gut or kidney, evokes significant betacytotropic effects; and 3) mannose- and fructose-induced enhancement of glucose disposal might be mediated by a factor(s) other than GIP."}
{"id": "PMID:479352", "title": "Acute effects of aminoglutethimide on testicular steroidogenesis in normal men.", "content": "Aminoglutethimide (AG), a known adrenal inhibitor, was administered acutely to normal men in order to study its effects on testicular steroidogenesis. Sixteen subjects between the ages of 21--30 yr received either placebo or 1250 mg AG in divided doses during a 24-h period. To reduce the contribution of adrenal steroids, adrenal function was inhibited in both groups of men by the administration of dexamethasone (2 mg) on the night of the experiment. As a result, mean morning plasma cortisol levels fell to less than 2 micrograms/100 ml. AG blunted the normal diurnal rise in testosterone, which was observed in men receiving placebo, and significantly suppressed the levels of this androgen at 0700 and 0900 h. Estradiol concentrations fell to a greater extent than those of testosterone. The differences between the placebo and drug treatment groups were significant at all time points except 1900 h. A pattern similar to that of estradiol was observed for plasma estrone. When the overall effect of AG administration was examined by analysis of variance, the differences in the levels of all three steroids produced by treatment were highly significant (P less than 0.01 to less than 0.001). After the inhibition of testosterone and estradiol levels, LH and FSH concentrations were significantly (P less than 0.01 and P less than 0.001, respectively) higher in men receiving AG than in those given placebo. However, the data exhibited a large variance due to pulsatile gonadotropin secretion. These observations suggested that AG induces an acute inhibition of testicular steroidogenesis and probably also of the aromatization of testosterone to estradiol.", "contents": "Acute effects of aminoglutethimide on testicular steroidogenesis in normal men. Aminoglutethimide (AG), a known adrenal inhibitor, was administered acutely to normal men in order to study its effects on testicular steroidogenesis. Sixteen subjects between the ages of 21--30 yr received either placebo or 1250 mg AG in divided doses during a 24-h period. To reduce the contribution of adrenal steroids, adrenal function was inhibited in both groups of men by the administration of dexamethasone (2 mg) on the night of the experiment. As a result, mean morning plasma cortisol levels fell to less than 2 micrograms/100 ml. AG blunted the normal diurnal rise in testosterone, which was observed in men receiving placebo, and significantly suppressed the levels of this androgen at 0700 and 0900 h. Estradiol concentrations fell to a greater extent than those of testosterone. The differences between the placebo and drug treatment groups were significant at all time points except 1900 h. A pattern similar to that of estradiol was observed for plasma estrone. When the overall effect of AG administration was examined by analysis of variance, the differences in the levels of all three steroids produced by treatment were highly significant (P less than 0.01 to less than 0.001). After the inhibition of testosterone and estradiol levels, LH and FSH concentrations were significantly (P less than 0.01 and P less than 0.001, respectively) higher in men receiving AG than in those given placebo. However, the data exhibited a large variance due to pulsatile gonadotropin secretion. These observations suggested that AG induces an acute inhibition of testicular steroidogenesis and probably also of the aromatization of testosterone to estradiol."}
{"id": "PMID:479353", "title": "Localization of aldosterone-producing adenoma by computed tomography.", "content": "Abdominal computed tomography (CT) was performed on nine patients with primary aldosteronism in an attempt to evaluate the utility of this noninvasive procedure in localizing aldosterone-producing adenomas. Confirmation of the diagnosis of primary aldosteronism was made by demonstrating elevated urinary aldosterone excretion, low PRA, and failure of plasma aldosterone to fall after acute saline load. Each patient had diagnostic lateralizing adrenal venous sampling and adrenal venography before unilateral adrenalectomy. The CT scan correctly predicted unilateral adenoma in the four patients whose tumors measured the largest in diameter at surgery. Bilateral tumors measuring 0.8 and 1.2 cm on scan (at or below the resolution capabilities of our scanner) were falsely predicted in two patients. The CT scan failed to identify an adrenal abnormality in three patients whose tumors measured 0.9, 1.0, and 1.0 cm in greatest diameter. It is concluded that at its current state of precision, the CT scan is of diagnostic utility in primary aldosteronism when a large adenoma is present. In our patients, the adrenal venogram and CT scan were equally accurate. The sampling of adrenal venous blood for aldosterone and cortisol remains the gold standard for localization of unilateral adenoma.", "contents": "Localization of aldosterone-producing adenoma by computed tomography. Abdominal computed tomography (CT) was performed on nine patients with primary aldosteronism in an attempt to evaluate the utility of this noninvasive procedure in localizing aldosterone-producing adenomas. Confirmation of the diagnosis of primary aldosteronism was made by demonstrating elevated urinary aldosterone excretion, low PRA, and failure of plasma aldosterone to fall after acute saline load. Each patient had diagnostic lateralizing adrenal venous sampling and adrenal venography before unilateral adrenalectomy. The CT scan correctly predicted unilateral adenoma in the four patients whose tumors measured the largest in diameter at surgery. Bilateral tumors measuring 0.8 and 1.2 cm on scan (at or below the resolution capabilities of our scanner) were falsely predicted in two patients. The CT scan failed to identify an adrenal abnormality in three patients whose tumors measured 0.9, 1.0, and 1.0 cm in greatest diameter. It is concluded that at its current state of precision, the CT scan is of diagnostic utility in primary aldosteronism when a large adenoma is present. In our patients, the adrenal venogram and CT scan were equally accurate. The sampling of adrenal venous blood for aldosterone and cortisol remains the gold standard for localization of unilateral adenoma."}
{"id": "PMID:479354", "title": "Direct radioiodination of estradiol for a sensitive radioimmunoassay.", "content": "17 beta-estradiol (E2) was iodinated with the chloramine-T method. The 125I-labelled material was purified with thin-layer chromatography. The major fraction was applied to a radioimmunoassay with an antiserum against estradiol-6-(0-carboxymethyl)oxime-BSA. The sensitivity of the assay was comparable to assays with 3H-E2 or 125I-histamine-E2 as tracers. The working antiserum dilution (1:1 000 000) was the same with 125I-E2 and 3H-E2 but lower than that with 125I-histamine-E2. The results indicate that iodination of the A-ring of estrogens will not cause significant reduction of the immunoreactivity when combined with an antiserum to a B-ring conjugate.", "contents": "Direct radioiodination of estradiol for a sensitive radioimmunoassay. 17 beta-estradiol (E2) was iodinated with the chloramine-T method. The 125I-labelled material was purified with thin-layer chromatography. The major fraction was applied to a radioimmunoassay with an antiserum against estradiol-6-(0-carboxymethyl)oxime-BSA. The sensitivity of the assay was comparable to assays with 3H-E2 or 125I-histamine-E2 as tracers. The working antiserum dilution (1:1 000 000) was the same with 125I-E2 and 3H-E2 but lower than that with 125I-histamine-E2. The results indicate that iodination of the A-ring of estrogens will not cause significant reduction of the immunoreactivity when combined with an antiserum to a B-ring conjugate."}
{"id": "PMID:479355", "title": "The role of thyronine in thyroid hormone metabolism.", "content": "Thryonine (T0) has been identified in human urine using gas chromatography-mass fragmentography (G.C.M.F.). In 22 normal individuals urinary T0 concentration was found to range between 8--25 nmol/24h. Assuming the mean normal thyroxine (T4) production rate to be approximately 100 nmol/24h, our findings indicate that less than 20% of this could be accounted for as urinary T0 excretion, thus supporting earlier findings that the peripheral metabolism of T4 is not limited solely to deiodination.", "contents": "The role of thyronine in thyroid hormone metabolism. Thryonine (T0) has been identified in human urine using gas chromatography-mass fragmentography (G.C.M.F.). In 22 normal individuals urinary T0 concentration was found to range between 8--25 nmol/24h. Assuming the mean normal thyroxine (T4) production rate to be approximately 100 nmol/24h, our findings indicate that less than 20% of this could be accounted for as urinary T0 excretion, thus supporting earlier findings that the peripheral metabolism of T4 is not limited solely to deiodination."}
{"id": "PMID:479356", "title": "Pseudomonas paucimobilis from a leg ulcer on a Japanese seaman.", "content": "Pseudomonas paucimobilis was isolated in pure culture from an ulcer on the leg of a Japanese seaman while in an Australian port. A description of the isolate is given. This may be the first report of a human infection in which this recently characterized species is implicated as a pathogen.", "contents": "Pseudomonas paucimobilis from a leg ulcer on a Japanese seaman. Pseudomonas paucimobilis was isolated in pure culture from an ulcer on the leg of a Japanese seaman while in an Australian port. A description of the isolate is given. This may be the first report of a human infection in which this recently characterized species is implicated as a pathogen."}
{"id": "PMID:479357", "title": "Simplified technique for sonication and processing of dental plaque samples.", "content": "A simplified method for processing dental plaque samples was devised and compared to previously used methods. Analysis of 36 nonstandardized subgingival plaque samples from various states of periodontal health and disease and 12 standardized supragingival plaque samples demonstrated that there was no significant difference between the recovery offered by the two techniques. Comparable recovery, increased convenience, and adaptation to the clinical setting suggests that implementation of this simplified technique may be of great value.", "contents": "Simplified technique for sonication and processing of dental plaque samples. A simplified method for processing dental plaque samples was devised and compared to previously used methods. Analysis of 36 nonstandardized subgingival plaque samples from various states of periodontal health and disease and 12 standardized supragingival plaque samples demonstrated that there was no significant difference between the recovery offered by the two techniques. Comparable recovery, increased convenience, and adaptation to the clinical setting suggests that implementation of this simplified technique may be of great value."}
{"id": "PMID:479358", "title": "Broth dilution minimum inhibitory concentrations: rationale for use of selected antimicrobial concentrations.", "content": "A microdilution susceptibility testing procedure utilizing selected, clinically relevant concentrations of a large number of antimicrobial agents is described. A qualitative code designed to facilitate interpretation of quantitative results is coupled with each antimicrobial concentration. Both the antimicrobial concentrations selected for testing and the assigned codes are based on published data regardling attainable antimicrobial levels in serum and urine.", "contents": "Broth dilution minimum inhibitory concentrations: rationale for use of selected antimicrobial concentrations. A microdilution susceptibility testing procedure utilizing selected, clinically relevant concentrations of a large number of antimicrobial agents is described. A qualitative code designed to facilitate interpretation of quantitative results is coupled with each antimicrobial concentration. Both the antimicrobial concentrations selected for testing and the assigned codes are based on published data regardling attainable antimicrobial levels in serum and urine."}
{"id": "PMID:479359", "title": "Survival of anaerobic bacteria in common laboratory diluents.", "content": "The survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. Bacteroides fragilis and Fusobacterium nucleatum showed excellent survival in all diluents including distilled water. Fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. Clostridium perfringens survived best in phosphate-buffered saline with gelatin. Peptococcus asaccharolyticus required gelatin added to the basic diluent, and Streptococcus intermedius showed excellent survival only in minimal essential medium with gelatin. These diluents could provide effective and economical alternatives to more complex and costly diluents often used in work with anaerobic bacteria.", "contents": "Survival of anaerobic bacteria in common laboratory diluents. The survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. Bacteroides fragilis and Fusobacterium nucleatum showed excellent survival in all diluents including distilled water. Fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. Clostridium perfringens survived best in phosphate-buffered saline with gelatin. Peptococcus asaccharolyticus required gelatin added to the basic diluent, and Streptococcus intermedius showed excellent survival only in minimal essential medium with gelatin. These diluents could provide effective and economical alternatives to more complex and costly diluents often used in work with anaerobic bacteria."}
{"id": "PMID:479360", "title": "Recovery of Angiostrongylus cantonensis from cerebrospinal fluid of a child with eosinophilic meningitis.", "content": "Viable Angiostrongylus cantonensis was recovered from the cerebrospinal fluid of a 17-month-old boy with eosinophilic meningitis. Neurological findings were minimal, and the child had an uneventful recovery.", "contents": "Recovery of Angiostrongylus cantonensis from cerebrospinal fluid of a child with eosinophilic meningitis. Viable Angiostrongylus cantonensis was recovered from the cerebrospinal fluid of a 17-month-old boy with eosinophilic meningitis. Neurological findings were minimal, and the child had an uneventful recovery."}
{"id": "PMID:479361", "title": "Fasle-positive macroscopic appearance of blood cultures in sorbitol-containing hypertonic medium.", "content": "Inability to rely on macroscopic examination as an aid in identifying positive blood cultures was encountered when a hypertonic medium containing sorbitol was tested in a comparative study with an isotonic blood culture medium.", "contents": "Fasle-positive macroscopic appearance of blood cultures in sorbitol-containing hypertonic medium. Inability to rely on macroscopic examination as an aid in identifying positive blood cultures was encountered when a hypertonic medium containing sorbitol was tested in a comparative study with an isotonic blood culture medium."}
{"id": "PMID:479362", "title": "Selective medium for Corynebacterium equi isolation.", "content": "The development of a selective medium for the isolation of Corynebacterium equi is described. The medium has been used to examine fecal samples from 127 horses of which 90 have been found to carry the organism.", "contents": "Selective medium for Corynebacterium equi isolation. The development of a selective medium for the isolation of Corynebacterium equi is described. The medium has been used to examine fecal samples from 127 horses of which 90 have been found to carry the organism."}
{"id": "PMID:479363", "title": "Further studies of the cellular fatty acid composition of Legionnaires disease bacteria.", "content": "The cellular fatty acid composition of 36 strains of the Legionnaires disease bacterium was determined by gas chromatography after growth on different media. The fatty acid profile of each strain was essentially identical on each medium and was characterized by large amounts (greater than 68%) of branched-chain acids.", "contents": "Further studies of the cellular fatty acid composition of Legionnaires disease bacteria. The cellular fatty acid composition of 36 strains of the Legionnaires disease bacterium was determined by gas chromatography after growth on different media. The fatty acid profile of each strain was essentially identical on each medium and was characterized by large amounts (greater than 68%) of branched-chain acids."}
{"id": "PMID:479364", "title": "Insulin binding to monocytes in trained athletes: changes in the resting state and after exercise.", "content": "Insulin binding to monocytes was examined in trained athletes (long distance runners) and in sedentary control subjects in the resting state and after 3 h of exercise at 40% of maximal aerobic power. At rest, specific binding of 125-I-insulin to monocytes was 69% higher in athletes than in sedentary controls and correlated with maximal aerobic power. The increase in insulin binding was primarily due to an increase in binding capacity. During acute exercise, insulin binding fell by 31% in athletes but rose by 35% in controls. The athletes had a smaller decline in plasma glucose and a lower respiratory exchange ratio during exercise than did controls. We conclude that physical training increases insulin binding to monocytes in the resting state but results in a fall in insulin binding during acute exercise. Changes in insulin binding in athletes thus may account for augmented insulin sensitivity at rest as well as a greater shift from carbohydrate to fat usage during exercise than is observed in untrained controls.", "contents": "Insulin binding to monocytes in trained athletes: changes in the resting state and after exercise. Insulin binding to monocytes was examined in trained athletes (long distance runners) and in sedentary control subjects in the resting state and after 3 h of exercise at 40% of maximal aerobic power. At rest, specific binding of 125-I-insulin to monocytes was 69% higher in athletes than in sedentary controls and correlated with maximal aerobic power. The increase in insulin binding was primarily due to an increase in binding capacity. During acute exercise, insulin binding fell by 31% in athletes but rose by 35% in controls. The athletes had a smaller decline in plasma glucose and a lower respiratory exchange ratio during exercise than did controls. We conclude that physical training increases insulin binding to monocytes in the resting state but results in a fall in insulin binding during acute exercise. Changes in insulin binding in athletes thus may account for augmented insulin sensitivity at rest as well as a greater shift from carbohydrate to fat usage during exercise than is observed in untrained controls."}
{"id": "PMID:479365", "title": "Effect of intact parathyroid hormone on hepatic glucose release in the dog.", "content": "The liver has been shown to remove parathyroid hormone (PTH) from its arterial circulation by a mechanism that is selective for the intact form of the peptide (PTH 1-84). The present studies demonstrate that PTH has biologic effects on the liver in vivo. Bovine PTH 1-84 stimulated hepatic glucose release in dogs with indwelling hepatic vein catheters from basal values of 31+/-8 to 68+/-9 mg/min per kg after bolus injections of PTH. The effect on hepatic glucose release was apparent by 5 min and persisted for the 80 min of observation. The NH(2)-terminal PTH fragment (syn b-PTH 1-34) had no effect. Bovine PTH 1-84 administered in doses designed to produce circulating levels of immunoreactive PTH similar to the endogenous levels observed in uremic dogs also increased the incorporation of (14)C from infused [(14)C]alanine into glucose, and increased estimated hepatic uptake of both chemical and [(14)C]alanine, while increasing hepatic glucose release. Thus, administration of \"physiologic levels\" of b-PTH 1-84 stimulated hepatic glucose release in part through increased gluconeogenesis in vivo, whereas syn b-PTH 1-34 had no demonstrable effect. Circulating levels of insulin rose after PTH administration, an increase which presumably represents a secondary response to the rise in glucose release. These results suggest that the liver is a target organ of PTH, and that PTH might potentially alter carbohydrate metabolism during hypersecretion. They also suggest that hepatic uptake of PTH may be related in part to production of a specific biologic effect rather than just simple peptide degradation.", "contents": "Effect of intact parathyroid hormone on hepatic glucose release in the dog. The liver has been shown to remove parathyroid hormone (PTH) from its arterial circulation by a mechanism that is selective for the intact form of the peptide (PTH 1-84). The present studies demonstrate that PTH has biologic effects on the liver in vivo. Bovine PTH 1-84 stimulated hepatic glucose release in dogs with indwelling hepatic vein catheters from basal values of 31+/-8 to 68+/-9 mg/min per kg after bolus injections of PTH. The effect on hepatic glucose release was apparent by 5 min and persisted for the 80 min of observation. The NH(2)-terminal PTH fragment (syn b-PTH 1-34) had no effect. Bovine PTH 1-84 administered in doses designed to produce circulating levels of immunoreactive PTH similar to the endogenous levels observed in uremic dogs also increased the incorporation of (14)C from infused [(14)C]alanine into glucose, and increased estimated hepatic uptake of both chemical and [(14)C]alanine, while increasing hepatic glucose release. Thus, administration of \"physiologic levels\" of b-PTH 1-84 stimulated hepatic glucose release in part through increased gluconeogenesis in vivo, whereas syn b-PTH 1-34 had no demonstrable effect. Circulating levels of insulin rose after PTH administration, an increase which presumably represents a secondary response to the rise in glucose release. These results suggest that the liver is a target organ of PTH, and that PTH might potentially alter carbohydrate metabolism during hypersecretion. They also suggest that hepatic uptake of PTH may be related in part to production of a specific biologic effect rather than just simple peptide degradation."}
{"id": "PMID:479366", "title": "Modification of hemoglobin H disease by sickle trait.", "content": "The rarity of hemoglobin (Hb) H disease in combination with sickle trait may be due in part to the absence of actual Hb H in individuals who, nonetheless, have inherited the deletion of three alpha-globin genes. We describe here a boy with persistent microcytic, hypochromic anemia despite adequate iron stores, who exhibited splenomegaly with a normal reticulocyte count and only rare inclusions in circulating erythrocytes. Starch gel electrophoresis and isoelectric focusing at age 5 yr showed 21% Hb S, persistent Hb Bart's, but no Hb H. Recticulocyte alpha/non-alpha globin chain synthesis ratio was 0.58 at age 5. The mother (Asian) had laboratory evidence of alpha-thalassemia trait and the father (Black) had sickle trait. The nature of alpha-thalassemia in this patient was investigated both by liquid hybridization and by the Southern method of gene mapping, in which DNA is digested with restriction endonucleases and the DNA fragments that contained the alpha-globin structural gene identified by hybridization with complementary DNA. The patient had only one alpha-globin structural gene, located in a DNA fragment shorter than that found in normal or alpha-thalassemia trait individuals, but similar to that present in other patients with Hb H disease. Morphologic studies of bone marrow by light and electron microscopy revealed erythroid hyperplasia with inclusions in polychromatic and orthochromatic erythroblasts, suggesting early precipitation of an unstable hemoglobin. The lack of demonstrable Hb H may be the result of both diminished amounts of beta(A) available for Hb H formation (since one beta-globin gene is beta(S)) and the greater affinity of alpha-chains for beta(A) than beta(S)-globin chains leading to the formation of relatively more Hb A than Hb S. The presence of a beta(S) gene may thus modify the usual clinical expression of Hb H disease.", "contents": "Modification of hemoglobin H disease by sickle trait. The rarity of hemoglobin (Hb) H disease in combination with sickle trait may be due in part to the absence of actual Hb H in individuals who, nonetheless, have inherited the deletion of three alpha-globin genes. We describe here a boy with persistent microcytic, hypochromic anemia despite adequate iron stores, who exhibited splenomegaly with a normal reticulocyte count and only rare inclusions in circulating erythrocytes. Starch gel electrophoresis and isoelectric focusing at age 5 yr showed 21% Hb S, persistent Hb Bart's, but no Hb H. Recticulocyte alpha/non-alpha globin chain synthesis ratio was 0.58 at age 5. The mother (Asian) had laboratory evidence of alpha-thalassemia trait and the father (Black) had sickle trait. The nature of alpha-thalassemia in this patient was investigated both by liquid hybridization and by the Southern method of gene mapping, in which DNA is digested with restriction endonucleases and the DNA fragments that contained the alpha-globin structural gene identified by hybridization with complementary DNA. The patient had only one alpha-globin structural gene, located in a DNA fragment shorter than that found in normal or alpha-thalassemia trait individuals, but similar to that present in other patients with Hb H disease. Morphologic studies of bone marrow by light and electron microscopy revealed erythroid hyperplasia with inclusions in polychromatic and orthochromatic erythroblasts, suggesting early precipitation of an unstable hemoglobin. The lack of demonstrable Hb H may be the result of both diminished amounts of beta(A) available for Hb H formation (since one beta-globin gene is beta(S)) and the greater affinity of alpha-chains for beta(A) than beta(S)-globin chains leading to the formation of relatively more Hb A than Hb S. The presence of a beta(S) gene may thus modify the usual clinical expression of Hb H disease."}
{"id": "PMID:479367", "title": "Mechanisms of gas exchange abnormality in patients with chronic obliterative pulmonary vascular disease.", "content": "We have examined the mechanisms of abnormal gas exchange in seven patients with chronic obliteration of the pulmonary vascular bed secondary to recurrent pulmonary emboli or idiopathic pulmonary hypertension. All of the patients had a widened alveolar-arterial oxygen gradient and four were significantly hypoxemic with arterial partial presssures of oxygen less than 80 torr. Using the technique of multiple inert gas elimination, we found that ventilation-perfusion (VA/Q) relationships were only minimally abnormal with a mean of 10% (range, 2--19%) of cardiac output perfusing abnormal units. These units consisted of shunt and units with VA/Q ratios less than 0.1. In addition, the dead space was found to be normal in each patient. There was no evidence for diffusion impairment, and the widened alveolar-arterial oxygen gradient was completely explained by VA/ inequality. Significant hypoxemia occurred only when VA/Q inequality was combined with a low mixed venous oxygen content.", "contents": "Mechanisms of gas exchange abnormality in patients with chronic obliterative pulmonary vascular disease. We have examined the mechanisms of abnormal gas exchange in seven patients with chronic obliteration of the pulmonary vascular bed secondary to recurrent pulmonary emboli or idiopathic pulmonary hypertension. All of the patients had a widened alveolar-arterial oxygen gradient and four were significantly hypoxemic with arterial partial presssures of oxygen less than 80 torr. Using the technique of multiple inert gas elimination, we found that ventilation-perfusion (VA/Q) relationships were only minimally abnormal with a mean of 10% (range, 2--19%) of cardiac output perfusing abnormal units. These units consisted of shunt and units with VA/Q ratios less than 0.1. In addition, the dead space was found to be normal in each patient. There was no evidence for diffusion impairment, and the widened alveolar-arterial oxygen gradient was completely explained by VA/ inequality. Significant hypoxemia occurred only when VA/Q inequality was combined with a low mixed venous oxygen content."}
{"id": "PMID:479368", "title": "Activation of human factor VII in plasma and in purified systems: roles of activated factor IX, kallikrein, and activated factor XII.", "content": "Factor VII can be activated, to a molecule giving shorter clotting times with tissue factor, by incubating plasma with kaolin or by clotting plasma. The mechanisms of activation differ. With kaolin, activated Factor XII (XII(a)) was the apparent principal activator. Thus, Factor VII was not activated in Factor XII-deficient plasma, was partially activated in prekallikrein and high-molecular weight kininogen (HMW kininogen)-deficient plasmas, but was activated in other deficient plasmas. After clotting, activated Factor IX (IX(a)) was the apparent principal activator. Thus, Factor VII was not activated in Factor XII-,HMW kininogen-, XI-, and IX-deficient plasmas, but was activated in Factor VIII-, X-, and V-deficient plasmas. In further studies, purified small-fragment Factor XII(a) (beta-XII(a)), kallikrein, and Factor IX(a) were added to partially purified Factor VII and to plasma. High concentrations of beta-XII(a) activated Factor VII in a purified system; much lower concentrations of beta-XII(a) activated Factor VII in normal plasma but not in prekallikrein or HWM kininogen-deficient plasmas. Kallikrein alone failed to activate partially purified Factor VII but did so when purified Factor IX was added. Kallikrein also activated Factor VII in normal, Factor XII-, and Factor IX-deficient plasmas. Purified Factor IX(a) activated partially purified Factor VII and had no additional indirect activating effect in the presence of plasma. These results demonstrate that both Factor XII(a) and Factor IX(a) directly activate human Factor VII, whereas kallikrein, through generation of Factor XII(a) and Factor IX(a), functions as an indirect activator of Factor VII.", "contents": "Activation of human factor VII in plasma and in purified systems: roles of activated factor IX, kallikrein, and activated factor XII. Factor VII can be activated, to a molecule giving shorter clotting times with tissue factor, by incubating plasma with kaolin or by clotting plasma. The mechanisms of activation differ. With kaolin, activated Factor XII (XII(a)) was the apparent principal activator. Thus, Factor VII was not activated in Factor XII-deficient plasma, was partially activated in prekallikrein and high-molecular weight kininogen (HMW kininogen)-deficient plasmas, but was activated in other deficient plasmas. After clotting, activated Factor IX (IX(a)) was the apparent principal activator. Thus, Factor VII was not activated in Factor XII-,HMW kininogen-, XI-, and IX-deficient plasmas, but was activated in Factor VIII-, X-, and V-deficient plasmas. In further studies, purified small-fragment Factor XII(a) (beta-XII(a)), kallikrein, and Factor IX(a) were added to partially purified Factor VII and to plasma. High concentrations of beta-XII(a) activated Factor VII in a purified system; much lower concentrations of beta-XII(a) activated Factor VII in normal plasma but not in prekallikrein or HWM kininogen-deficient plasmas. Kallikrein alone failed to activate partially purified Factor VII but did so when purified Factor IX was added. Kallikrein also activated Factor VII in normal, Factor XII-, and Factor IX-deficient plasmas. Purified Factor IX(a) activated partially purified Factor VII and had no additional indirect activating effect in the presence of plasma. These results demonstrate that both Factor XII(a) and Factor IX(a) directly activate human Factor VII, whereas kallikrein, through generation of Factor XII(a) and Factor IX(a), functions as an indirect activator of Factor VII."}
{"id": "PMID:479369", "title": "Pituitary gland: site of short-loop feedback for luteinizing hormone in the rabbit.", "content": "The development of a species specific radioimmunoassay for rabbit luteinizing hormone (LH) has permitted the direct demonstration of LH feedback control of LH secretion (short-loop feedback control). In previous studies we showed that small bolus injections of human LH (hLH) intravenously administered to castrate female rabbits suppressed rabbit LH for 20-30 min. Human LH had no effect on rabbit follicle-stimulating hormone secretion. This control system was responsive to amounts of hLH estimated to be present in blood of eugonadal men and women. These studies were designed to determine whether this feedback control was exerted at a pituitary or hypothalamic level. Two groups of studies were carried out: (a) in vivo studies: Rabbit LH was quantified in the blood of castrated female New Zealand White rabbits receiving either constant hLH perfusion (2.75 IU/min) or saline perfusion, plus a bolus injection of 0.5, 6, or 20 mug of gonadotropin-releasing hormone (GnRH). Human LH decreased the response to 6 and 20 mug of GnRH by 31 and 36%, respectively, and abolished the response to 0.5 mug, GnRH. (b) in vitro studies: Rabbit pituitary slices were incubated in the presence of medium alone, medium plus hLH (25 mIU/ml), medium plus GnRH (20 mug/ml), and medium plus both GnRH and hLH. hLH decreased basal rabbit LH release into the medium and abolished GnRH-stimulated rabbit LH release. hLH had no effect on rabbit follicle-stimulating hormone release. From these results we conclude that a direct and specific feedback control of LH on LH exists at a pituitary level.", "contents": "Pituitary gland: site of short-loop feedback for luteinizing hormone in the rabbit. The development of a species specific radioimmunoassay for rabbit luteinizing hormone (LH) has permitted the direct demonstration of LH feedback control of LH secretion (short-loop feedback control). In previous studies we showed that small bolus injections of human LH (hLH) intravenously administered to castrate female rabbits suppressed rabbit LH for 20-30 min. Human LH had no effect on rabbit follicle-stimulating hormone secretion. This control system was responsive to amounts of hLH estimated to be present in blood of eugonadal men and women. These studies were designed to determine whether this feedback control was exerted at a pituitary or hypothalamic level. Two groups of studies were carried out: (a) in vivo studies: Rabbit LH was quantified in the blood of castrated female New Zealand White rabbits receiving either constant hLH perfusion (2.75 IU/min) or saline perfusion, plus a bolus injection of 0.5, 6, or 20 mug of gonadotropin-releasing hormone (GnRH). Human LH decreased the response to 6 and 20 mug of GnRH by 31 and 36%, respectively, and abolished the response to 0.5 mug, GnRH. (b) in vitro studies: Rabbit pituitary slices were incubated in the presence of medium alone, medium plus hLH (25 mIU/ml), medium plus GnRH (20 mug/ml), and medium plus both GnRH and hLH. hLH decreased basal rabbit LH release into the medium and abolished GnRH-stimulated rabbit LH release. hLH had no effect on rabbit follicle-stimulating hormone release. From these results we conclude that a direct and specific feedback control of LH on LH exists at a pituitary level."}
{"id": "PMID:479370", "title": "Functional characterization of left ventricular segmental responses during the initial 24 h and 1 wk after experimental canine myocardial infarction.", "content": "Characterization of the temporal evolution of resting segmental function and inotropic reserve after coronary occlusion may be important in evaluating attempts to salvage ischemic but non-necrotic myocardium. Accordingly, we chronically implanted up to six pairs of pulse-transit piezoelectric crystals in the left ventricular myocardium of dogs to measure segmental wall thickness. Segments were separated into groups according to the loss of net systolic thickening (NET) at 5 min postocclusion of the left anterior descending coronary artery in awake, unsedated dogs. Group 1 included segments with NET values of 67--100+ (percent control); group 2 between 67 and 0; and group 3 less than 0 (paradoxical motion). 5 min after coronary occlusion, group 1 NET was 92 +/- 5% (SEM) although significant decreases occurred in NET in group 2 (36 +/- 4%) and group 3 segments (-33 +/- 5%). Between 5 min and 24 h after coronary occlusion, no further significant changes occurred in NET in groups 1, 2, and 3 crystals. Some segments underwent further functional deterioration between 24 h and 1 wk after left anterior descending coronary artery occlusion, although no overall change occurred in segments with mild to moderate ischemic dysfunction. Segments with NET less than 0 at 24 h, on the other hand, exhibited a reduction in aneurysmal bulging between 24 h and 1 wk from -41 +/- 10 to -23 +/- 11% (n = 12, P = 0.02). Inotropic reserve was assessed with postextrasystolic potentiation (PESP) in 14 dogs, and with infusions of dopamine (11 dogs), and isoproterenol (13 dogs). PESP was the most potent intervention and produced a significant augmentation in NET in group 2 crystals at 1, 2, 4, 6,8, and 24 h after coronary occlusion but only at 1 and 2 h in NET in group 3 crystals. Thus, following experimental coronary occlusion, the evolution of ischemic segmental dysfunction is dynamic and variable. A significant degree of inotropic reserve, as assessed by PESP, dopamine, and isoproterenol, exists in segments with moderate ischemic dysfunction for 24 h but for only 2 h after coronary occlusion in those segments with the most severe ischemic dysfunction. In addition, at least some segmental sites with mild to moderate ischemic dysfunction at 24 h deteriorate further between 24 h and 1 wk after experimental coronary occlusion.", "contents": "Functional characterization of left ventricular segmental responses during the initial 24 h and 1 wk after experimental canine myocardial infarction. Characterization of the temporal evolution of resting segmental function and inotropic reserve after coronary occlusion may be important in evaluating attempts to salvage ischemic but non-necrotic myocardium. Accordingly, we chronically implanted up to six pairs of pulse-transit piezoelectric crystals in the left ventricular myocardium of dogs to measure segmental wall thickness. Segments were separated into groups according to the loss of net systolic thickening (NET) at 5 min postocclusion of the left anterior descending coronary artery in awake, unsedated dogs. Group 1 included segments with NET values of 67--100+ (percent control); group 2 between 67 and 0; and group 3 less than 0 (paradoxical motion). 5 min after coronary occlusion, group 1 NET was 92 +/- 5% (SEM) although significant decreases occurred in NET in group 2 (36 +/- 4%) and group 3 segments (-33 +/- 5%). Between 5 min and 24 h after coronary occlusion, no further significant changes occurred in NET in groups 1, 2, and 3 crystals. Some segments underwent further functional deterioration between 24 h and 1 wk after left anterior descending coronary artery occlusion, although no overall change occurred in segments with mild to moderate ischemic dysfunction. Segments with NET less than 0 at 24 h, on the other hand, exhibited a reduction in aneurysmal bulging between 24 h and 1 wk from -41 +/- 10 to -23 +/- 11% (n = 12, P = 0.02). Inotropic reserve was assessed with postextrasystolic potentiation (PESP) in 14 dogs, and with infusions of dopamine (11 dogs), and isoproterenol (13 dogs). PESP was the most potent intervention and produced a significant augmentation in NET in group 2 crystals at 1, 2, 4, 6,8, and 24 h after coronary occlusion but only at 1 and 2 h in NET in group 3 crystals. Thus, following experimental coronary occlusion, the evolution of ischemic segmental dysfunction is dynamic and variable. A significant degree of inotropic reserve, as assessed by PESP, dopamine, and isoproterenol, exists in segments with moderate ischemic dysfunction for 24 h but for only 2 h after coronary occlusion in those segments with the most severe ischemic dysfunction. In addition, at least some segmental sites with mild to moderate ischemic dysfunction at 24 h deteriorate further between 24 h and 1 wk after experimental coronary occlusion."}
{"id": "PMID:479371", "title": "Effect of anticonvulsant drugs on the rate of folate catabolism in mice.", "content": "An increase in folate catabolism has been suggested as the cause of the folate deficiency observed in many clinical conditions, including chronic anticonvulsant therapy. Previous studies have shown that the radioactive catabolites, excreted after an equilibration period of 3 d, consisted exclusively of folates that had been cleaved to produce pteridines and p-aminobenzoylglutamate, most of which was excreted as acetamidobenzoylglutamate. We have developed an experimental animal model using mice to determine the rate of catabolism of [3H]pteroylglutamate (folic acid) by the quantitative estimation of [3H]p-aminobenzoylglutamate and [3H]acetamidobenzoylglutamate in urine. Administration of diphenylhydantoin at three different doses (0.5, 20, and 50 mg/kg) significantly increased the rate of catabolism as measured by an increase in both the mean daily excretion and the cumulative excretion of these catabolites. Administration of intramuscular phenobarbitone on the other hand, did not affect the rate of catabolism, when compared with controls.", "contents": "Effect of anticonvulsant drugs on the rate of folate catabolism in mice. An increase in folate catabolism has been suggested as the cause of the folate deficiency observed in many clinical conditions, including chronic anticonvulsant therapy. Previous studies have shown that the radioactive catabolites, excreted after an equilibration period of 3 d, consisted exclusively of folates that had been cleaved to produce pteridines and p-aminobenzoylglutamate, most of which was excreted as acetamidobenzoylglutamate. We have developed an experimental animal model using mice to determine the rate of catabolism of [3H]pteroylglutamate (folic acid) by the quantitative estimation of [3H]p-aminobenzoylglutamate and [3H]acetamidobenzoylglutamate in urine. Administration of diphenylhydantoin at three different doses (0.5, 20, and 50 mg/kg) significantly increased the rate of catabolism as measured by an increase in both the mean daily excretion and the cumulative excretion of these catabolites. Administration of intramuscular phenobarbitone on the other hand, did not affect the rate of catabolism, when compared with controls."}
{"id": "PMID:479372", "title": "Site of substrate stimulation of jejunal sucrase in the rat.", "content": "To identify the site of stimulation of sucrase by a sucrose diet, changes in sucrase-specific activity of jejunal mucosa were studied after introduction of sucrose diet to carbohydrate-deprived rats. Results were correlated with simultaneous changes in villus gradients of sucrase-specific activity. Simultaneous with the introduction of sucrose diet, [(3)H]thymidine (100 muCi) was administered intravenously, and rates of cell migration measured during adaptation to the new diet. After a 72-h fast, rats fed sucrose diet for 6, 12, or 18 h showed no change in sucrase-specific activity in either whole mucosa or villus gradients. However, within 18-24 h after starting a sucrose diet, there was a marked rise in whole mucosal sucrase-specific activity above fasting values (99 +/- 14 vs. 38 +/- 4 muM glucose/min per g protein, P < 0.001) in association with the development of a region of increased activity at the lower villus (154 +/- 22 vs. 60 +/- 9 muM glucose/min per g protein, P < 0.02, but with no change in villus tip activity (56 +/- 5 vs. 46 +/- 8 muM glucose/min per g protein). Similar changes were seen in animals fed 24 h of sucrose diet after a 72-h carbohydratefree diet. Fasted animals fed sucrose diet for 36 h had increased sucrase-specific activity at the villus tip (144 +/- 11 muM glucose/min per g protein) as well as at the lower villus region, and this pattern persisted at 1 wk of sucrose diet. Maximal activity patterns for isomaltase and maltase paralleled those for sucrase, but the villus gradients for lactase were unaffected by sucrose diet. The region of maximal sucrase-specific activity always coincided with or followed the leading edge of radioactivity as determined by liquid scintillation counting. Therefore, sucrose-mediated changes in sucrase activity of the jejunal mucosa in the rat appear to be initiated at the level of the crypt epithelial cell and are expressed after a latent period of 18-24 h during which these cells mature and migrate toward the villus tip.", "contents": "Site of substrate stimulation of jejunal sucrase in the rat. To identify the site of stimulation of sucrase by a sucrose diet, changes in sucrase-specific activity of jejunal mucosa were studied after introduction of sucrose diet to carbohydrate-deprived rats. Results were correlated with simultaneous changes in villus gradients of sucrase-specific activity. Simultaneous with the introduction of sucrose diet, [(3)H]thymidine (100 muCi) was administered intravenously, and rates of cell migration measured during adaptation to the new diet. After a 72-h fast, rats fed sucrose diet for 6, 12, or 18 h showed no change in sucrase-specific activity in either whole mucosa or villus gradients. However, within 18-24 h after starting a sucrose diet, there was a marked rise in whole mucosal sucrase-specific activity above fasting values (99 +/- 14 vs. 38 +/- 4 muM glucose/min per g protein, P < 0.001) in association with the development of a region of increased activity at the lower villus (154 +/- 22 vs. 60 +/- 9 muM glucose/min per g protein, P < 0.02, but with no change in villus tip activity (56 +/- 5 vs. 46 +/- 8 muM glucose/min per g protein). Similar changes were seen in animals fed 24 h of sucrose diet after a 72-h carbohydratefree diet. Fasted animals fed sucrose diet for 36 h had increased sucrase-specific activity at the villus tip (144 +/- 11 muM glucose/min per g protein) as well as at the lower villus region, and this pattern persisted at 1 wk of sucrose diet. Maximal activity patterns for isomaltase and maltase paralleled those for sucrase, but the villus gradients for lactase were unaffected by sucrose diet. The region of maximal sucrase-specific activity always coincided with or followed the leading edge of radioactivity as determined by liquid scintillation counting. Therefore, sucrose-mediated changes in sucrase activity of the jejunal mucosa in the rat appear to be initiated at the level of the crypt epithelial cell and are expressed after a latent period of 18-24 h during which these cells mature and migrate toward the villus tip."}
{"id": "PMID:479373", "title": "Erythrocyte adenosine deaminase deficiency without immunodeficiency. Evidence for an unstable mutant enzyme.", "content": "Inherited deficiency of the purine salvage enzyme adenosine deaminase (ADA) gives rise to a syndrome of severe combined immunodeficiency (SCID). We have studied a 2.5-yr-old immunologically normal child who had been found to lack ADA in his erythrocytes during New York State screening of normal newborns. His erythrocytes were not detectably less deficient in ADA than erythrocytes of ADA(-)-SCID patients. In contrast, his lymphocytes and cultured long-term lymphoid cells contained appreciably greater ADA activity than those from patients with ADA(-)-SCID. This residual ADA activity had a normal molecular weight and K(m) but was markedly unstable at 56 degrees C. His residual erythrocytes-ADA activity also appeared to have diminished stability in vivo. ADA activity in lymphoid line cells of a previously reported erythrocyte-ADA-deficient!Kung tribesman was found to contain 50% of normal activity and to exhibit diminished stability at 56 degrees C. ATP content of erythrocytes from both partially ADA-deficient individuals was detectably greater than normal (12.3 and 6.1 vs. normal of 2.6 nmol/ml packed erythrocytes). However, the dATP content was insignificant compared to that found in erythrocytes of ADA(-)-SCID patients (400-1,000 nmol/ml packed erythrocytes). The New York patient, in contrast to normals, excreted detectable amounts of deoxyadenosine, but this was <2% of deoxyadenosine excreted by ADA(-)-SCID patients. Thus, the residual enzyme in cells other than erythrocytes appears to be sufficient to almost totally prevent accumulation of toxic metabolites.", "contents": "Erythrocyte adenosine deaminase deficiency without immunodeficiency. Evidence for an unstable mutant enzyme. Inherited deficiency of the purine salvage enzyme adenosine deaminase (ADA) gives rise to a syndrome of severe combined immunodeficiency (SCID). We have studied a 2.5-yr-old immunologically normal child who had been found to lack ADA in his erythrocytes during New York State screening of normal newborns. His erythrocytes were not detectably less deficient in ADA than erythrocytes of ADA(-)-SCID patients. In contrast, his lymphocytes and cultured long-term lymphoid cells contained appreciably greater ADA activity than those from patients with ADA(-)-SCID. This residual ADA activity had a normal molecular weight and K(m) but was markedly unstable at 56 degrees C. His residual erythrocytes-ADA activity also appeared to have diminished stability in vivo. ADA activity in lymphoid line cells of a previously reported erythrocyte-ADA-deficient!Kung tribesman was found to contain 50% of normal activity and to exhibit diminished stability at 56 degrees C. ATP content of erythrocytes from both partially ADA-deficient individuals was detectably greater than normal (12.3 and 6.1 vs. normal of 2.6 nmol/ml packed erythrocytes). However, the dATP content was insignificant compared to that found in erythrocytes of ADA(-)-SCID patients (400-1,000 nmol/ml packed erythrocytes). The New York patient, in contrast to normals, excreted detectable amounts of deoxyadenosine, but this was <2% of deoxyadenosine excreted by ADA(-)-SCID patients. Thus, the residual enzyme in cells other than erythrocytes appears to be sufficient to almost totally prevent accumulation of toxic metabolites."}
{"id": "PMID:479374", "title": "Temperature perturbation studies of sarcoplasmic reticulum from malignant hyperthermia pig muscle.", "content": "The effects of varying temperatures from 25 degrees to 37 degrees C on calcium binding characteristics of sarcoplasmic reticulum from malignant hyperthermia-susceptible (MHS) and control pig muscle were examined. Two groups of MHS pigs were included: those with high susceptibility to malignant hyperthermia (MHS group) and a cross-bred, less susceptible group (MHX). At 25 degrees C, calcium binding was lower for MHS than for controls and MHX. As temperature was increased by 2 degrees C jumps, calcium binding decreased in all sarcoplasmic reticulum fractions. At 35 degrees C a sharp decrease in calcium binding occurred in the MHS and MHX fractions. The sharp decrease in calcium binding at 35 degrees C differentiated the MHS and MHX fractions from controls. The initial velocity (Vi) for calcium binding was lower in MHS fractions between 25 degrees and 35 degrees C when compared with MHX and controls. All fractions had increased Vi values as temperature increased from 25 degrees to 35 degrees C. From 35 degrees to 39 degrees Vi for controls increased markedly. In contrast, Vi for the MHX fraction decreased as temperature exceeded 35 degrees C. These temperature effects on calcium binding characteristics of sarcoplasmic reticulum from MHS and MHX muscle may be indicative of a membrane transition that impairs calcium binding.", "contents": "Temperature perturbation studies of sarcoplasmic reticulum from malignant hyperthermia pig muscle. The effects of varying temperatures from 25 degrees to 37 degrees C on calcium binding characteristics of sarcoplasmic reticulum from malignant hyperthermia-susceptible (MHS) and control pig muscle were examined. Two groups of MHS pigs were included: those with high susceptibility to malignant hyperthermia (MHS group) and a cross-bred, less susceptible group (MHX). At 25 degrees C, calcium binding was lower for MHS than for controls and MHX. As temperature was increased by 2 degrees C jumps, calcium binding decreased in all sarcoplasmic reticulum fractions. At 35 degrees C a sharp decrease in calcium binding occurred in the MHS and MHX fractions. The sharp decrease in calcium binding at 35 degrees C differentiated the MHS and MHX fractions from controls. The initial velocity (Vi) for calcium binding was lower in MHS fractions between 25 degrees and 35 degrees C when compared with MHX and controls. All fractions had increased Vi values as temperature increased from 25 degrees to 35 degrees C. From 35 degrees to 39 degrees Vi for controls increased markedly. In contrast, Vi for the MHX fraction decreased as temperature exceeded 35 degrees C. These temperature effects on calcium binding characteristics of sarcoplasmic reticulum from MHS and MHX muscle may be indicative of a membrane transition that impairs calcium binding."}
{"id": "PMID:479375", "title": "Neutrophil-binding immunoglobulin G in systemic lupus erythematosus.", "content": "The objectives of these studies were to quantify the amounts of immunoglobulin (Ig)G bound to peripheral blood neutrophils from patients with systemic lupus erythematosus (SLE) and to determine the contributions of soluble immune complexes or anticell antibodies to the levels of IgG neutrophil-binding activity in SLE sera. Neutrophil-bound IgG, determined by a sensitive antiglobulin inhibition assay, was elevated in 7 out of 14 SLE patients compared with values obtained in 23 normal controls. The levels of IgG neutrophil-binding activity in sera were elevated in 22 of 38 patients with SLE over the values seen with 36 normal sera. No correlation was found between the peripheral blood neutrophil counts in the SLE patients and the values for IgG adherent to the cells or serum cell-binding activity. The sera from 18 patients with SLE were fractionated by gel filtration. Elevated levels of IgG neutrophil-binding activity were found in 11 of the 18 G-200 excluded pools and in 13 of the G-200 IgG pools. In nine sera elevated levels were observed in both pools. F(ab')2 fragments of IgG from SLE sera bound to normal polymorphonuclear leukocytes in greater amounts than F(ab')2 fragments of IgG from normal sera. A significant correlation existed between the values of IgG neutrophil-binding activity found in SLE sera and those obtained with both the G-200 excluded and IgG pools. Sucrose density gradient fractionation of four sera from SLE patients confirmed the presence of both large (greater than 19S) and intermediate-sized (7S-19S) cell-binding immune complexes as well as of monomeric IgG antibodies to neutrophils. The levels of IgG neutrophil-binding activity in the SLE sera correlated well with the results obtained with the Raji cell assay for immune complexes as well as with the titer of antibodies to nuclear antigens. These data indicate that circulating neutrophils from patients with SLE commonly have increased amounts of cell-bound IGG. The elevated levels of IgG neutrophil-binding activity in the sera of these patients are caused by both soluble immune complexes and antibodies reactive with neutrophils.", "contents": "Neutrophil-binding immunoglobulin G in systemic lupus erythematosus. The objectives of these studies were to quantify the amounts of immunoglobulin (Ig)G bound to peripheral blood neutrophils from patients with systemic lupus erythematosus (SLE) and to determine the contributions of soluble immune complexes or anticell antibodies to the levels of IgG neutrophil-binding activity in SLE sera. Neutrophil-bound IgG, determined by a sensitive antiglobulin inhibition assay, was elevated in 7 out of 14 SLE patients compared with values obtained in 23 normal controls. The levels of IgG neutrophil-binding activity in sera were elevated in 22 of 38 patients with SLE over the values seen with 36 normal sera. No correlation was found between the peripheral blood neutrophil counts in the SLE patients and the values for IgG adherent to the cells or serum cell-binding activity. The sera from 18 patients with SLE were fractionated by gel filtration. Elevated levels of IgG neutrophil-binding activity were found in 11 of the 18 G-200 excluded pools and in 13 of the G-200 IgG pools. In nine sera elevated levels were observed in both pools. F(ab')2 fragments of IgG from SLE sera bound to normal polymorphonuclear leukocytes in greater amounts than F(ab')2 fragments of IgG from normal sera. A significant correlation existed between the values of IgG neutrophil-binding activity found in SLE sera and those obtained with both the G-200 excluded and IgG pools. Sucrose density gradient fractionation of four sera from SLE patients confirmed the presence of both large (greater than 19S) and intermediate-sized (7S-19S) cell-binding immune complexes as well as of monomeric IgG antibodies to neutrophils. The levels of IgG neutrophil-binding activity in the SLE sera correlated well with the results obtained with the Raji cell assay for immune complexes as well as with the titer of antibodies to nuclear antigens. These data indicate that circulating neutrophils from patients with SLE commonly have increased amounts of cell-bound IGG. The elevated levels of IgG neutrophil-binding activity in the sera of these patients are caused by both soluble immune complexes and antibodies reactive with neutrophils."}
{"id": "PMID:479376", "title": "Staphylococci-induced human platelet injury mediated by protein A and immunoglobulin G Fc fragment receptor.", "content": "Bloodstream infections with staphylococci are accompanied by thromboembolic complications. We have studied the mechanism of the interaction of staphylococci with human blood platelets. Staphylococci that possess protein A, a bacterial receptor for the Fc fragment of immunoglobulin G (IgG), caused aggregation of human platelets in whole plasma accompanied by release of [(3)H]serotonin. These reactions were time and concentration dependent, requiring two or more staphylococci per platelet to give maximal response within 5 min. The interaction between staphylococci and platelets required the presence of cell wall-bound protein A and of IgG with an intact Fc fragment. It did not require an intact complement system. Cell wall-bound protein A (solid phase) was capable of aggregating human platelets in whole plasma. In contrast, free, solubilized protein A (fluid phase) did not cause measurable aggregation, and release of [(3)H]serotonin was reduced. An excess of free, solubilized protein A blocked aggregation of human platelets induced by staphylococci in whole plasma. The role of the Fc fragment of IgG in the staphylococci-human platelet interaction was demonstrated by an experiment in which free, isolated Fc fragment blocked aggregation of platelets in whole plasma induced by staphylococci. Furthermore, binding of (125)I-protein A to human platelets was demonstrated in the presence of complete IgG with intact Fc fragment but not in the presence of the F(ab)(2) fragment. Binding of the protein A-IgG complex to the human platelet Fc receptor was paralleled by the release of [(3)H]serotonin. These results represent a novel example of the interaction of two phylogenetically different Fc receptors, one on prokaryotic staphylococci and the other on human platelets. Their common ligand, IgG, is amplified by one Fc receptor (protein A) to react with another Fc receptor present on human platelets, which results in membrane-mediated aggregation and release reaction occurring in whole plasma. This mechanism can be of significance in the pathomechanism of thromboembolic complications at the site(s) of intravascular staphylococcal infection.", "contents": "Staphylococci-induced human platelet injury mediated by protein A and immunoglobulin G Fc fragment receptor. Bloodstream infections with staphylococci are accompanied by thromboembolic complications. We have studied the mechanism of the interaction of staphylococci with human blood platelets. Staphylococci that possess protein A, a bacterial receptor for the Fc fragment of immunoglobulin G (IgG), caused aggregation of human platelets in whole plasma accompanied by release of [(3)H]serotonin. These reactions were time and concentration dependent, requiring two or more staphylococci per platelet to give maximal response within 5 min. The interaction between staphylococci and platelets required the presence of cell wall-bound protein A and of IgG with an intact Fc fragment. It did not require an intact complement system. Cell wall-bound protein A (solid phase) was capable of aggregating human platelets in whole plasma. In contrast, free, solubilized protein A (fluid phase) did not cause measurable aggregation, and release of [(3)H]serotonin was reduced. An excess of free, solubilized protein A blocked aggregation of human platelets induced by staphylococci in whole plasma. The role of the Fc fragment of IgG in the staphylococci-human platelet interaction was demonstrated by an experiment in which free, isolated Fc fragment blocked aggregation of platelets in whole plasma induced by staphylococci. Furthermore, binding of (125)I-protein A to human platelets was demonstrated in the presence of complete IgG with intact Fc fragment but not in the presence of the F(ab)(2) fragment. Binding of the protein A-IgG complex to the human platelet Fc receptor was paralleled by the release of [(3)H]serotonin. These results represent a novel example of the interaction of two phylogenetically different Fc receptors, one on prokaryotic staphylococci and the other on human platelets. Their common ligand, IgG, is amplified by one Fc receptor (protein A) to react with another Fc receptor present on human platelets, which results in membrane-mediated aggregation and release reaction occurring in whole plasma. This mechanism can be of significance in the pathomechanism of thromboembolic complications at the site(s) of intravascular staphylococcal infection."}
{"id": "PMID:479377", "title": "Renal adaptation to a low phosphate diet in rats.", "content": "The major renal adaptive changes in response to selective dietary phosphate restriction are a marked reduction in urinary excretion of phosphate and an increased urinary excretion of calcium; at the cellular level, there is selective increase in renal cortical brush border membrane phosphate uptake and increase in specific activity of alkaline phosphatase. In the present study we examined whether these functional and biochemical adaptive changes could be blocked by drugs known to inhibit protein synthesis. Administration of actinomycin D or cycloheximide to rats switched from a diet with normal phosphate content (0.7%) to a diet with low (0.07%) phosphate content either completely (actinomycin D) or partially (cycloheximide) prevented the expected decrease in urinary excretion of phosphate and increase in the urinary excretion of calcium. The specific activity of alkaline phosphatase measured in crude membrane fraction (washed 100,000 g pellet) from renal cortical homogenate in animals fed a low phosphate diet and treated with actinomycin D or with cycloheximide was significantly lower than in control animals also on a low phosphate diet receiving placebo; but there were no differences between treated and untreated animals in the activities of two other brush border enzymes, gamma-glutamyltransferase and leucine aminopeptidase. Actinomycin D administered to rats maintained on a normal phosphate diet throughout the course of the experiment caused an increase in the urinary excretion of phosphate on the last (6th) day of the experiment but did not change urinary excretion of calcium. In acute clearance experiments, infusion of actinomycin D to rats adapted to a low phosphate diet did not increase fractional excretion of phosphate. In separate experiments, using the same dietary protocol as above, brush border membrane fraction (vesicles) was prepared from renal cortex of rats sacrificed at the end of the experiment. In this preparation Na(+)-dependent (32)Pi and d-[(3)H]glucose uptake and activities of brush border enzymes membrane were determined. Brush border membrane vesicles prepared from rats fed a low phosphate diet showed significantly higher Na(+)-dependent (32)Pi uptake compared with rats fed a normal phosphate diet. This increase in (32)Pi uptake was completely prevented when rats on a low phosphate diet were simultaneously treated with actinomycin D. These differences were specific for (32)Pi transport as no differences were observed in d-[(3)H]glucose uptake among the three groups. There was a positive correlation (r = 0.82, P < 0.01) between (32)Pi uptake and specific activity of alkaline phosphatase measured in aliquots of the same brush border membranes, whereas no such correlation was observed with two other brush border membrane enzymes gamma-glutamyltransferase and leucine aminopeptidase. These observations show that actinomycin D prevents both the functional and cellular renal adaptive changes induced by a low phosphate diet. Taken together, these observations suggest that renal adaptation to a low phosphate diet could be prevented by inhibition of de novo protein synthesis.", "contents": "Renal adaptation to a low phosphate diet in rats. The major renal adaptive changes in response to selective dietary phosphate restriction are a marked reduction in urinary excretion of phosphate and an increased urinary excretion of calcium; at the cellular level, there is selective increase in renal cortical brush border membrane phosphate uptake and increase in specific activity of alkaline phosphatase. In the present study we examined whether these functional and biochemical adaptive changes could be blocked by drugs known to inhibit protein synthesis. Administration of actinomycin D or cycloheximide to rats switched from a diet with normal phosphate content (0.7%) to a diet with low (0.07%) phosphate content either completely (actinomycin D) or partially (cycloheximide) prevented the expected decrease in urinary excretion of phosphate and increase in the urinary excretion of calcium. The specific activity of alkaline phosphatase measured in crude membrane fraction (washed 100,000 g pellet) from renal cortical homogenate in animals fed a low phosphate diet and treated with actinomycin D or with cycloheximide was significantly lower than in control animals also on a low phosphate diet receiving placebo; but there were no differences between treated and untreated animals in the activities of two other brush border enzymes, gamma-glutamyltransferase and leucine aminopeptidase. Actinomycin D administered to rats maintained on a normal phosphate diet throughout the course of the experiment caused an increase in the urinary excretion of phosphate on the last (6th) day of the experiment but did not change urinary excretion of calcium. In acute clearance experiments, infusion of actinomycin D to rats adapted to a low phosphate diet did not increase fractional excretion of phosphate. In separate experiments, using the same dietary protocol as above, brush border membrane fraction (vesicles) was prepared from renal cortex of rats sacrificed at the end of the experiment. In this preparation Na(+)-dependent (32)Pi and d-[(3)H]glucose uptake and activities of brush border enzymes membrane were determined. Brush border membrane vesicles prepared from rats fed a low phosphate diet showed significantly higher Na(+)-dependent (32)Pi uptake compared with rats fed a normal phosphate diet. This increase in (32)Pi uptake was completely prevented when rats on a low phosphate diet were simultaneously treated with actinomycin D. These differences were specific for (32)Pi transport as no differences were observed in d-[(3)H]glucose uptake among the three groups. There was a positive correlation (r = 0.82, P < 0.01) between (32)Pi uptake and specific activity of alkaline phosphatase measured in aliquots of the same brush border membranes, whereas no such correlation was observed with two other brush border membrane enzymes gamma-glutamyltransferase and leucine aminopeptidase. These observations show that actinomycin D prevents both the functional and cellular renal adaptive changes induced by a low phosphate diet. Taken together, these observations suggest that renal adaptation to a low phosphate diet could be prevented by inhibition of de novo protein synthesis."}
{"id": "PMID:479379", "title": "The one-point method in predicting dosage regimen in case of hepatic and/or renal failure in presence or absence of change in volume of distribution.", "content": "In some clinical situations such as in acute myocardial or coronary infarction, liver disease, renal failure, hypoalbuminemia, displacement from protein binding, etc., both the volume of distribution and the elimination rate may change. The determination of the creatinine clearance may be too time consuming, and the serum creatinine concentration may be an unreliable indicator in some cases (acute renal failure, uremia, muscular diseases, old age, etc). Neither indicates change in elimination by extrarenal routes (metabolism in liver disease). The \"repeated one-point method\" is presented as clinical tool to calculate the dosage regimen for patients where differences in Vd and kel can be expected. The method is based on the evaluation of one blood sample each in the first two dosing intervals after administration of a test dose. The \"repeated one-point method\" is compared with the previously published \"one-point method.\"", "contents": "The one-point method in predicting dosage regimen in case of hepatic and/or renal failure in presence or absence of change in volume of distribution. In some clinical situations such as in acute myocardial or coronary infarction, liver disease, renal failure, hypoalbuminemia, displacement from protein binding, etc., both the volume of distribution and the elimination rate may change. The determination of the creatinine clearance may be too time consuming, and the serum creatinine concentration may be an unreliable indicator in some cases (acute renal failure, uremia, muscular diseases, old age, etc). Neither indicates change in elimination by extrarenal routes (metabolism in liver disease). The \"repeated one-point method\" is presented as clinical tool to calculate the dosage regimen for patients where differences in Vd and kel can be expected. The method is based on the evaluation of one blood sample each in the first two dosing intervals after administration of a test dose. The \"repeated one-point method\" is compared with the previously published \"one-point method.\""}
{"id": "PMID:479381", "title": "Cefamandole pharmacokinetics and dosage adjustments in relation to renal function.", "content": "The pharmacokinetic characteristics of cefamandole were determined after intravenous administration of a 1-Gm dose to 10 subjects with normal renal function, 10 patients with stabilized renal failure, and five chronic nephritic patients included in a intermittent hemodialysis program. In normal subjects, biological half-life (t1/2) averaged 0.94 hour, the overall elimination rate constant (Ke) was 0.7378 (hr-1), total clearance (Ct) was 223 ml/min/1.73 m2, renal clearance (Cr) was 164 ml/min/1.73 m2, and urine recovery of cefamandole over the 6 hours following a dose amounted to 74 per cent of the administered dose. In patients with stabilized renal failure and in patients on hemodialysis, biological half-life was markedly increased, with a theoretical value of 10.4 hours in case of a creatinine clearance of zero. The amount of antibiotic extracted over a 6-hour dialysis period accounted for 29 per cent of the cefamandole present in the vascular compartment at the beginning of the dialysis procedure. A significant correlation was established between the values of Ke and creatinine clearances, Ccr: Ke = 0.0289 + 0.0063Ccr (r = 0.937). This relationship was used to calculate the loading dose (LD), maintenance doses (D), and dosage intervals (tau) with regard to renal function. From these data recommendations regarding the adjustment of cefamandole dosage to the renal status can be made.", "contents": "Cefamandole pharmacokinetics and dosage adjustments in relation to renal function. The pharmacokinetic characteristics of cefamandole were determined after intravenous administration of a 1-Gm dose to 10 subjects with normal renal function, 10 patients with stabilized renal failure, and five chronic nephritic patients included in a intermittent hemodialysis program. In normal subjects, biological half-life (t1/2) averaged 0.94 hour, the overall elimination rate constant (Ke) was 0.7378 (hr-1), total clearance (Ct) was 223 ml/min/1.73 m2, renal clearance (Cr) was 164 ml/min/1.73 m2, and urine recovery of cefamandole over the 6 hours following a dose amounted to 74 per cent of the administered dose. In patients with stabilized renal failure and in patients on hemodialysis, biological half-life was markedly increased, with a theoretical value of 10.4 hours in case of a creatinine clearance of zero. The amount of antibiotic extracted over a 6-hour dialysis period accounted for 29 per cent of the cefamandole present in the vascular compartment at the beginning of the dialysis procedure. A significant correlation was established between the values of Ke and creatinine clearances, Ccr: Ke = 0.0289 + 0.0063Ccr (r = 0.937). This relationship was used to calculate the loading dose (LD), maintenance doses (D), and dosage intervals (tau) with regard to renal function. From these data recommendations regarding the adjustment of cefamandole dosage to the renal status can be made."}
{"id": "PMID:479384", "title": "A double-blind study of dezocine in cancer pain.", "content": "A double-blind crossover comparison of intramuscularly administration of 10 mg dezocine (Wy 16225), 10 mg morphine, and placebo in ten patients with cancer pain has shown that a dezocine single dose is superior to placebo in producing relief of moderate to severe pain. No side effects were observed.", "contents": "A double-blind study of dezocine in cancer pain. A double-blind crossover comparison of intramuscularly administration of 10 mg dezocine (Wy 16225), 10 mg morphine, and placebo in ten patients with cancer pain has shown that a dezocine single dose is superior to placebo in producing relief of moderate to severe pain. No side effects were observed."}
{"id": "PMID:479385", "title": "The clinical analgesic efficacy of oral nefopam hydrochloride.", "content": "The analgesic efficacy of 60 and 120 mg nefopam hydrochloride was compared to 650 mg aspirin and placebo in a double-blind single-dose study. Oral doses were administered to 120 patients suffering from acute postsurgical or fracture pain. All active medications demonstrated analgesic activity in comparison to placebo. Patients on 120 mg nefopam obtained the greatest degree of analgesia. Side effects were minor and did not interfere with the course of therapy. The incidence of side effects (sweating, nausea, and lightheadedness) was greater on 120 mg nefopam than on 650 mg aspirin).", "contents": "The clinical analgesic efficacy of oral nefopam hydrochloride. The analgesic efficacy of 60 and 120 mg nefopam hydrochloride was compared to 650 mg aspirin and placebo in a double-blind single-dose study. Oral doses were administered to 120 patients suffering from acute postsurgical or fracture pain. All active medications demonstrated analgesic activity in comparison to placebo. Patients on 120 mg nefopam obtained the greatest degree of analgesia. Side effects were minor and did not interfere with the course of therapy. The incidence of side effects (sweating, nausea, and lightheadedness) was greater on 120 mg nefopam than on 650 mg aspirin)."}
{"id": "PMID:479391", "title": "Inhibition of suckling in weaning-age rats: a possible serotonergic mechanism.", "content": "The nature, development, and specificity of serotonergic involvement in the control of suckling behavior in rat pups from 10 to 35 days of age were studied. During development, suckling normally declines after 10 days and is abandoned after 30 days. It was found that (a) methysergide, a serotonin (5-HT) receptor blocker, reinstated suckling behavior in pups 15 days of age and older but had no effect on the suckling of 10-day old pups, (b) quipazine, a 5-HT receptor agonist, inhibited suckling of pups 10 days of age and older, (c) methysergide pretreatment blocked the quipazine inhibition of suckling, and (d) metergoline, another 5-HT blocker, also stimulated suckling, and fenfluramine, a 5-HT releaser, blocked suckling in deprived pups. Together, these data support the hypothesis that a serotonergic inhibitory mechanism modulates the suckling of weaning-age rats. These pharmacological manipulations of 5-HT appear to alter specific components of suckling behavior involved in its initiation and maintenance but do not appear to alter a general hunger system.", "contents": "Inhibition of suckling in weaning-age rats: a possible serotonergic mechanism. The nature, development, and specificity of serotonergic involvement in the control of suckling behavior in rat pups from 10 to 35 days of age were studied. During development, suckling normally declines after 10 days and is abandoned after 30 days. It was found that (a) methysergide, a serotonin (5-HT) receptor blocker, reinstated suckling behavior in pups 15 days of age and older but had no effect on the suckling of 10-day old pups, (b) quipazine, a 5-HT receptor agonist, inhibited suckling of pups 10 days of age and older, (c) methysergide pretreatment blocked the quipazine inhibition of suckling, and (d) metergoline, another 5-HT blocker, also stimulated suckling, and fenfluramine, a 5-HT releaser, blocked suckling in deprived pups. Together, these data support the hypothesis that a serotonergic inhibitory mechanism modulates the suckling of weaning-age rats. These pharmacological manipulations of 5-HT appear to alter specific components of suckling behavior involved in its initiation and maintenance but do not appear to alter a general hunger system."}
{"id": "PMID:479392", "title": "Taste aversions to mother's milk: the age-related role of nursing in acquisition and expression of a learned association.", "content": "The development of taste aversion learning to novel cues contained in mother's milk was examined in rat pups. Pups receiving distinctive mild by experimenter-delivered oral infusions followed by toxicosis formed an aversion to the dam's diet. Robust aversions were learned as early as Day 10 and were retained for at least 11 days. When the same distinctive milk was obtained directly from a foster mother through nursing, however, only weanling-age pups (over 20 days) formed an aversion. X-ray analysis of nipple location in the mouths of suckling pups suggested that pups between the ages of 10 and 21 days receive milk at a similar tongue locus. Flavored milk was then delivered at specific time intervals in controlled quantities through tongue cannulas implanted at loci corresponding to the nipple position shown by the X-rays. Cannulated preweanling pups that were attached to a nipple during milk delivery failed to associate the taste cue with illness, whereas both preweanlings off the nipple and weanlings on the nipple acquired aversions to the taste cue in the milk. The evidence obtained in these experiments suggests that pups of all ages are incapable of expressing a taste aversion in a nursing situation and that preweanling pups in particular are also dificient in acquiring aversions within a suckling context. The inability of preweanling pups to acquire taste aversions in a nursing situation appears to result from a failure to associate taste cues with illness rather than a failure to detect taste cues obtained from a nipple.", "contents": "Taste aversions to mother's milk: the age-related role of nursing in acquisition and expression of a learned association. The development of taste aversion learning to novel cues contained in mother's milk was examined in rat pups. Pups receiving distinctive mild by experimenter-delivered oral infusions followed by toxicosis formed an aversion to the dam's diet. Robust aversions were learned as early as Day 10 and were retained for at least 11 days. When the same distinctive milk was obtained directly from a foster mother through nursing, however, only weanling-age pups (over 20 days) formed an aversion. X-ray analysis of nipple location in the mouths of suckling pups suggested that pups between the ages of 10 and 21 days receive milk at a similar tongue locus. Flavored milk was then delivered at specific time intervals in controlled quantities through tongue cannulas implanted at loci corresponding to the nipple position shown by the X-rays. Cannulated preweanling pups that were attached to a nipple during milk delivery failed to associate the taste cue with illness, whereas both preweanlings off the nipple and weanlings on the nipple acquired aversions to the taste cue in the milk. The evidence obtained in these experiments suggests that pups of all ages are incapable of expressing a taste aversion in a nursing situation and that preweanling pups in particular are also dificient in acquiring aversions within a suckling context. The inability of preweanling pups to acquire taste aversions in a nursing situation appears to result from a failure to associate taste cues with illness rather than a failure to detect taste cues obtained from a nipple."}
{"id": "PMID:479393", "title": "Approach and withdrawal analysis of the effects of hypothalamic stimulation and lesions in rats.", "content": "In a paradigm in which rats would both initiate and terminate hypothalamic stimulation, \"purely\" rewarding lateral hypothalamic and \"purely\" aversive medial hypothalamic electrodes were identified. Subjects were then given a series of tests designed to assess the effects of brain stimulation on approach and withdrawal behaviors. Lateral hypothalamic stimulation facilitated approach behavior and suppressed withdrawal behaviors, whereas medial hypothalamic stimulation produced largely the opposite effects. No serious motor deficits due to stimulation were detected with either type of electrode. In a second experiment, the approach-withdrawal effects of bilateral lateral hypothalamic lesions were tested and found to suppress approach behaviors and facilitate withdrawal behaviors. Other neurological examinations revealed motor deficits, but these deficits do not account for the specific pattern of results observed on the approach-withdrawal tests. These approach-withdrawal phenomena are interpreted in terms of altering a natural balance between approach and withdrawl behavior facilitating systems in the lateral and medial hypothalamus, respectively.", "contents": "Approach and withdrawal analysis of the effects of hypothalamic stimulation and lesions in rats. In a paradigm in which rats would both initiate and terminate hypothalamic stimulation, \"purely\" rewarding lateral hypothalamic and \"purely\" aversive medial hypothalamic electrodes were identified. Subjects were then given a series of tests designed to assess the effects of brain stimulation on approach and withdrawal behaviors. Lateral hypothalamic stimulation facilitated approach behavior and suppressed withdrawal behaviors, whereas medial hypothalamic stimulation produced largely the opposite effects. No serious motor deficits due to stimulation were detected with either type of electrode. In a second experiment, the approach-withdrawal effects of bilateral lateral hypothalamic lesions were tested and found to suppress approach behaviors and facilitate withdrawal behaviors. Other neurological examinations revealed motor deficits, but these deficits do not account for the specific pattern of results observed on the approach-withdrawal tests. These approach-withdrawal phenomena are interpreted in terms of altering a natural balance between approach and withdrawl behavior facilitating systems in the lateral and medial hypothalamus, respectively."}
{"id": "PMID:479394", "title": "Effects of genetic vestibular defects on behavior related to spatial orientation and emotionality.", "content": "A large battery of behavioral tests was administered to normal mice and to mice with varying degrees of otoconial agenesis due to genes affecting vestibular development. Many significant differences were found, but a factor analysis revealed that the variance on the 11 best tests could be accounted for in terms of two underlying variables. Factor I, the more important of the two, was associated with activity, habituation, and spontaneous alternation. Factor II appeared to represent a fear of new stimuli or situations. In both cases factor scores were highly related to the degree of otoconial deficiency. One subgroup of mice with severe otoconial agenesis displayed hyperactivity and a total absence of either habituation or spontaneous alternation. In these animals, brain and body development were stunted, and the reactions to amphetamine and physostigmine were opposite to those seen in normal mice. The results support the idea that the static organs contribute importantly to spatial orientation and suggest that early-onset vestibular defects can result in profound alterations of emotionality.", "contents": "Effects of genetic vestibular defects on behavior related to spatial orientation and emotionality. A large battery of behavioral tests was administered to normal mice and to mice with varying degrees of otoconial agenesis due to genes affecting vestibular development. Many significant differences were found, but a factor analysis revealed that the variance on the 11 best tests could be accounted for in terms of two underlying variables. Factor I, the more important of the two, was associated with activity, habituation, and spontaneous alternation. Factor II appeared to represent a fear of new stimuli or situations. In both cases factor scores were highly related to the degree of otoconial deficiency. One subgroup of mice with severe otoconial agenesis displayed hyperactivity and a total absence of either habituation or spontaneous alternation. In these animals, brain and body development were stunted, and the reactions to amphetamine and physostigmine were opposite to those seen in normal mice. The results support the idea that the static organs contribute importantly to spatial orientation and suggest that early-onset vestibular defects can result in profound alterations of emotionality."}
{"id": "PMID:479395", "title": "Reduction of ontogenetic retention decrements in rats by pretraining stressful experiences.", "content": "Pretraining stressful experiences, either similar to or qualitatively different from the conditioning unconditioned stimulus, reduced ontogenetic retention decrements without directly influencing response acquisition. Rats that received pretreatment with footshock, hypthermia, or restraint on Days 16, 17, and 18 of age showed substantially improved long-term retention of conditioned fear learned at 20 days of age. It was also found that preshock enhanced retention of both an appetitive approach response and punishment of the approach response. Several experiments involving extinction and undertraining manipulations indicated that the facilitation of retention was not directly attributable to acquisition strength.", "contents": "Reduction of ontogenetic retention decrements in rats by pretraining stressful experiences. Pretraining stressful experiences, either similar to or qualitatively different from the conditioning unconditioned stimulus, reduced ontogenetic retention decrements without directly influencing response acquisition. Rats that received pretreatment with footshock, hypthermia, or restraint on Days 16, 17, and 18 of age showed substantially improved long-term retention of conditioned fear learned at 20 days of age. It was also found that preshock enhanced retention of both an appetitive approach response and punishment of the approach response. Several experiments involving extinction and undertraining manipulations indicated that the facilitation of retention was not directly attributable to acquisition strength."}
{"id": "PMID:479396", "title": "Evidence for neural inhibition in bittersweet taste mixtures.", "content": "Three lines of evidence from psychophysical experiments implied that mutual suppression of bitter and sweet tastes is due to neural inhibition rather than chemical interactions in solution or competition of molecules for common receptor sites. Removal of sweetness from bittersweet mixtures caused the bitterness to increase. This was accomplished by adaptation to sucrose or by treatment with Gymnema sylvestre, neither of which affect the concentration of sucrose on the tongue. Such increases in the bitterness of mixtures, independent of the concentration of the sweet masking substance, are difficult to reconcile with suppression by means of chemical interactions. Similar dependence of suppression on perceived intensity (and independence from concentration) was observed with mixtures of phyenylthiocarbamide and sucrose. Tasters of phenylthiocarbamide showed stronger suppression of sweetness than nontasters. This result was also inconsistent with molecular interactions causing suppression, which would have resulted in the same degree of suppression for the two groups. Instead, these findings support neural explanations of mixture suppression, such as antidromic inhibition or occlusion.", "contents": "Evidence for neural inhibition in bittersweet taste mixtures. Three lines of evidence from psychophysical experiments implied that mutual suppression of bitter and sweet tastes is due to neural inhibition rather than chemical interactions in solution or competition of molecules for common receptor sites. Removal of sweetness from bittersweet mixtures caused the bitterness to increase. This was accomplished by adaptation to sucrose or by treatment with Gymnema sylvestre, neither of which affect the concentration of sucrose on the tongue. Such increases in the bitterness of mixtures, independent of the concentration of the sweet masking substance, are difficult to reconcile with suppression by means of chemical interactions. Similar dependence of suppression on perceived intensity (and independence from concentration) was observed with mixtures of phyenylthiocarbamide and sucrose. Tasters of phenylthiocarbamide showed stronger suppression of sweetness than nontasters. This result was also inconsistent with molecular interactions causing suppression, which would have resulted in the same degree of suppression for the two groups. Instead, these findings support neural explanations of mixture suppression, such as antidromic inhibition or occlusion."}
{"id": "PMID:479397", "title": "Olfactory stimulation induces filial preferences for huddling in rat pups.", "content": "Rat pups of all ages huddle with conspecifics, but the senosory control of contact behavior changes ontogenetically. Thermal cues control huddling until about Day 15, at which time species' odors become the dominant stimulus. The present experiments indicate that the filial response to conspecifics is dependent on olfactory experience. A synthetic chemical scent was added to the smells of the dam from Day 1 to Day 20 postpartum. Standardized videographic tests were used to assess the development of huddling preference. Preferences for nest-typical smells emerged by Day 15 in pups from both scented and nonscented litters. Pups from scented nests preferred to huddle with a scented stimulus rat, whereas control pups preferred a nonadulterated rat stimulus. Additional testing indicated that the affiliative preferences were specific to rearing odor and were not based on decreased aversion to test scents, or on disrupted olfactory discrimination. The ontogeny of species-typical contact behavior is discussed in terms of the induction of a perceptual preference that is based on early odor stimulation.", "contents": "Olfactory stimulation induces filial preferences for huddling in rat pups. Rat pups of all ages huddle with conspecifics, but the senosory control of contact behavior changes ontogenetically. Thermal cues control huddling until about Day 15, at which time species' odors become the dominant stimulus. The present experiments indicate that the filial response to conspecifics is dependent on olfactory experience. A synthetic chemical scent was added to the smells of the dam from Day 1 to Day 20 postpartum. Standardized videographic tests were used to assess the development of huddling preference. Preferences for nest-typical smells emerged by Day 15 in pups from both scented and nonscented litters. Pups from scented nests preferred to huddle with a scented stimulus rat, whereas control pups preferred a nonadulterated rat stimulus. Additional testing indicated that the affiliative preferences were specific to rearing odor and were not based on decreased aversion to test scents, or on disrupted olfactory discrimination. The ontogeny of species-typical contact behavior is discussed in terms of the induction of a perceptual preference that is based on early odor stimulation."}
{"id": "PMID:479398", "title": "Thermal homeostasis in pregnant rats during heat stress.", "content": "Rats exposed to inescapable heat stress maintained a controlled hyperthermia while increasing heat loss by cutaneous vasodilatation and by grooming behavior. In nonpregnant rats, the evaporation of saliva groomed onto the body surfaces increased exponentially as a function of ambient temperature above 36 degrees C. This was associated with a decrease in the body temperature threshold for salivary secretion from the submaxillary gland, which then began at approximately the same body temperature as cutaneous vasodilatation. In addition, the pregnant rats maintained a lower level of controlled hyperthermia during heat stress than did nonpregnant rats. This appeared to result from a decreased production of metabolic heat, reduced insulation on the ventral surface, and an increased motivation to keep cool during heat stress. These changes met the increased need for thermolysis during pregnancy and provided for thermal homeostasis both in the pregnant rat and in the unborn fetuses.", "contents": "Thermal homeostasis in pregnant rats during heat stress. Rats exposed to inescapable heat stress maintained a controlled hyperthermia while increasing heat loss by cutaneous vasodilatation and by grooming behavior. In nonpregnant rats, the evaporation of saliva groomed onto the body surfaces increased exponentially as a function of ambient temperature above 36 degrees C. This was associated with a decrease in the body temperature threshold for salivary secretion from the submaxillary gland, which then began at approximately the same body temperature as cutaneous vasodilatation. In addition, the pregnant rats maintained a lower level of controlled hyperthermia during heat stress than did nonpregnant rats. This appeared to result from a decreased production of metabolic heat, reduced insulation on the ventral surface, and an increased motivation to keep cool during heat stress. These changes met the increased need for thermolysis during pregnancy and provided for thermal homeostasis both in the pregnant rat and in the unborn fetuses."}
{"id": "PMID:479399", "title": "Human visual evoked responses are related to heart rate.", "content": "The purpose of the present experiment was to determine the relation between changes in heart rate and averaged evoked potentials. Sixteen male subjects with variable heart rates received 50 flashes of light during fast heart beats, 50 during slow heart beats, and 50 during midrange heart rate level. Evoked responses were recorded from the scalp overlying the right and left occipital lobes. Heart rate, respiration, eye movements, and cephalic blood flow were also recorded. The results indicated that spontaneous changes in heart rate are related to changes in visual evoked responses and that this relation differs for the two cerebral hemispheres. Furthermore, cephalic pulse amplitude was largest following slow heart beats and smallest following fast heart beats, which suggests that changes in heart rate are related to changes in cerebrovascular as well as electrocortical activity. The results are discussed in terms of formulations derived from behavioral studies regarding the relation between cardiovascular activity and attentional processes.", "contents": "Human visual evoked responses are related to heart rate. The purpose of the present experiment was to determine the relation between changes in heart rate and averaged evoked potentials. Sixteen male subjects with variable heart rates received 50 flashes of light during fast heart beats, 50 during slow heart beats, and 50 during midrange heart rate level. Evoked responses were recorded from the scalp overlying the right and left occipital lobes. Heart rate, respiration, eye movements, and cephalic blood flow were also recorded. The results indicated that spontaneous changes in heart rate are related to changes in visual evoked responses and that this relation differs for the two cerebral hemispheres. Furthermore, cephalic pulse amplitude was largest following slow heart beats and smallest following fast heart beats, which suggests that changes in heart rate are related to changes in cerebrovascular as well as electrocortical activity. The results are discussed in terms of formulations derived from behavioral studies regarding the relation between cardiovascular activity and attentional processes."}
{"id": "PMID:479400", "title": "Neuronal unit activity in the abducens nucleus during classical conditioning of the nictitating membrane response in the rabbit (Oryctolagus cuniculus).", "content": "Neuronal unit activity was recorded from the abducens (6th nerve) nucleus, the \"final common path,\" during classical conditioning of the nictitating membrane (NM) response in the rabbit, with the use of a tone conditioned stimulus, an air puff unconditioned stimulus (UCS), 250-msec interstimulus interval, and 60-sec intertrial interval. Animals were given 2 days of conditioning training (104 trials in eight blocks per day) and 1 day of extinction. Control animals were given comparable periods of stimulus presentations, explicitly unpaired. Activity of small clusters of units--\"multiple unit\" recording--was compared with the amplitude-time course of the NM response. Between-blocks comparisons of neural and behavioral responses indicated an essentially perfect correlation during acquisition of the conditioned response (Day 1, r = .99; Day 2, r = .98) and a slightly lower correlation during extinction (r = .93) for the conditioning animals. Within-blocks comparisons indicated a close correspondence between the histograms of unit activity and the amplitude-time course of the NM response for the conditioning animals in all phase of training and for the control animals in the UCS trial blocks.", "contents": "Neuronal unit activity in the abducens nucleus during classical conditioning of the nictitating membrane response in the rabbit (Oryctolagus cuniculus). Neuronal unit activity was recorded from the abducens (6th nerve) nucleus, the \"final common path,\" during classical conditioning of the nictitating membrane (NM) response in the rabbit, with the use of a tone conditioned stimulus, an air puff unconditioned stimulus (UCS), 250-msec interstimulus interval, and 60-sec intertrial interval. Animals were given 2 days of conditioning training (104 trials in eight blocks per day) and 1 day of extinction. Control animals were given comparable periods of stimulus presentations, explicitly unpaired. Activity of small clusters of units--\"multiple unit\" recording--was compared with the amplitude-time course of the NM response. Between-blocks comparisons of neural and behavioral responses indicated an essentially perfect correlation during acquisition of the conditioned response (Day 1, r = .99; Day 2, r = .98) and a slightly lower correlation during extinction (r = .93) for the conditioning animals. Within-blocks comparisons indicated a close correspondence between the histograms of unit activity and the amplitude-time course of the NM response for the conditioning animals in all phase of training and for the control animals in the UCS trial blocks."}
{"id": "PMID:479401", "title": "Ontogeny of cholinergic mediation of behaviors in the rat.", "content": "In a series of six experiments, cholinergic mediation of behavior was studied in immature rats. It was found that although scopolamine disrupted discriminative choice behavior in both 15- and 23-day-old rat pups, it increased latency to choice in 15-day-olds and decreased latency to choice in 23-day-olds. This disruption of discriminated choice behavior was not due to differential shock thresholds or differences in locomotor activity between drug-treated and control animals, nor was it specific to a T-maze shock-escape discrimination task. These results suggest that central cholinergic mediation of different behaviors may mature at different rates.", "contents": "Ontogeny of cholinergic mediation of behaviors in the rat. In a series of six experiments, cholinergic mediation of behavior was studied in immature rats. It was found that although scopolamine disrupted discriminative choice behavior in both 15- and 23-day-old rat pups, it increased latency to choice in 15-day-olds and decreased latency to choice in 23-day-olds. This disruption of discriminated choice behavior was not due to differential shock thresholds or differences in locomotor activity between drug-treated and control animals, nor was it specific to a T-maze shock-escape discrimination task. These results suggest that central cholinergic mediation of different behaviors may mature at different rates."}
{"id": "PMID:479402", "title": "Mother rats interact differently with male and female offspring.", "content": "Lactating Long-Evans rats were observed to interact differently with male and female pups during the first 18 days postpartum. Differences in the mother's behavior were related to the gender composition of her litter (GHL), to the sex of a single introduced pup, and to the sex of individual pups within her litter. Major differences were the greater time spent in licking the anogenital region of own male pups and the greater stimulation of anogenital licking by male foster pups, an effect that did not interact with GHL or age of pup. The GHL interacted with day of testing to affect nest building and time spent near pups.", "contents": "Mother rats interact differently with male and female offspring. Lactating Long-Evans rats were observed to interact differently with male and female pups during the first 18 days postpartum. Differences in the mother's behavior were related to the gender composition of her litter (GHL), to the sex of a single introduced pup, and to the sex of individual pups within her litter. Major differences were the greater time spent in licking the anogenital region of own male pups and the greater stimulation of anogenital licking by male foster pups, an effect that did not interact with GHL or age of pup. The GHL interacted with day of testing to affect nest building and time spent near pups."}
{"id": "PMID:479403", "title": "Comparison of effects of small lesions in posterodorsal septum on spontaneous and rerun correction (contingently reinforced) alternation in rats.", "content": "In the first part of the experiment, characteristics of T-maze alternation behavior were compared by giving one group of 11 rats seven sessions under conditions of \"rerun correction\" (contingent reinforcement for alternation) and another group of 11 rats the same number of sessions with noncontingent reinforcements, i.e., entering either goal arm of the T-maze was reinforced. Both groups alternated significantly above chance, and the difference in medians between groups was small but significant in later sessions. However, rats given rerun correction training quickly came against the \"ceiling\" of 100% alternation, and individual differences were small. The scores of rats run under conditions of \"spontaneous\" alternation were more variable, both within and between rats. In the second part of the experiment, six rats in each group received small electrolytic lesions in the posterodorsal septum. There were five operated-control rats from each group. After 2-wk postoperative recovery all rats were again given seven sessions in the T-maze. Under rerun correction procedure (contingent reinforcement), the controls retained near-perfect alternation scores. Most animals with lesions dropped to chance levels but recovered to control level over the seven sessions. Rats tested under spontaneous alternation conditions behaved quite differently. The control group continued to alternate significantly, but rats with lesions significantly alternated less and did not recover.", "contents": "Comparison of effects of small lesions in posterodorsal septum on spontaneous and rerun correction (contingently reinforced) alternation in rats. In the first part of the experiment, characteristics of T-maze alternation behavior were compared by giving one group of 11 rats seven sessions under conditions of \"rerun correction\" (contingent reinforcement for alternation) and another group of 11 rats the same number of sessions with noncontingent reinforcements, i.e., entering either goal arm of the T-maze was reinforced. Both groups alternated significantly above chance, and the difference in medians between groups was small but significant in later sessions. However, rats given rerun correction training quickly came against the \"ceiling\" of 100% alternation, and individual differences were small. The scores of rats run under conditions of \"spontaneous\" alternation were more variable, both within and between rats. In the second part of the experiment, six rats in each group received small electrolytic lesions in the posterodorsal septum. There were five operated-control rats from each group. After 2-wk postoperative recovery all rats were again given seven sessions in the T-maze. Under rerun correction procedure (contingent reinforcement), the controls retained near-perfect alternation scores. Most animals with lesions dropped to chance levels but recovered to control level over the seven sessions. Rats tested under spontaneous alternation conditions behaved quite differently. The control group continued to alternate significantly, but rats with lesions significantly alternated less and did not recover."}
{"id": "PMID:479404", "title": "Active regulation to be lean by rats with ventromedial hypothalamic lesions.", "content": "Adult female Long-Evans rats with direct-current electrolytic or radio-frequency thermocoagulatory lesions of the ventromedial hypothalamus (VMH) lived on pellet fragments or a powdered chow containing as much as 1.2% quinine sulfate by weight or lived in Skinner boxes with 45-mg Noyes pellets delivered contingent on fixed ratios (FR) of lever pressing up to FR 128. As had been found previously for intact rats, the body weights maintained by VMH rats were determined by the percentage of quinine sulfate in or the contingency of reinforcement of the food on which they lived. Even when the rats lived on highly adulterated or response-contingent food and were lean, they ate more of that food when the ambient temperature was reduced and less of that food during several weeks of forced feeding of eggnog by gavage. Weight maintenance in the cold and caloric compensation during forced feeding were as precise for VMH rats eating highly adulterated chow or Noyes pellets contingent on high fixed ratios of lever pressing as for VMH rats eating typical laboratory chow ad lib, even though the former rats at the time maintained weights no greater than intact rats and the latter rats at the time were grossly obese. Furthermore, regulation in the cold or during forced feeding was in general only a little less precise for the rats with lesions than for intact rats. It may be as characteristic of VMH rats that they eat to become lean and remain lean as that they eat to be obese. The diets of both intact and VMH rats in some manner determine the adiposities that rats will defend against caloric challenge.", "contents": "Active regulation to be lean by rats with ventromedial hypothalamic lesions. Adult female Long-Evans rats with direct-current electrolytic or radio-frequency thermocoagulatory lesions of the ventromedial hypothalamus (VMH) lived on pellet fragments or a powdered chow containing as much as 1.2% quinine sulfate by weight or lived in Skinner boxes with 45-mg Noyes pellets delivered contingent on fixed ratios (FR) of lever pressing up to FR 128. As had been found previously for intact rats, the body weights maintained by VMH rats were determined by the percentage of quinine sulfate in or the contingency of reinforcement of the food on which they lived. Even when the rats lived on highly adulterated or response-contingent food and were lean, they ate more of that food when the ambient temperature was reduced and less of that food during several weeks of forced feeding of eggnog by gavage. Weight maintenance in the cold and caloric compensation during forced feeding were as precise for VMH rats eating highly adulterated chow or Noyes pellets contingent on high fixed ratios of lever pressing as for VMH rats eating typical laboratory chow ad lib, even though the former rats at the time maintained weights no greater than intact rats and the latter rats at the time were grossly obese. Furthermore, regulation in the cold or during forced feeding was in general only a little less precise for the rats with lesions than for intact rats. It may be as characteristic of VMH rats that they eat to become lean and remain lean as that they eat to be obese. The diets of both intact and VMH rats in some manner determine the adiposities that rats will defend against caloric challenge."}
{"id": "PMID:479405", "title": "Naloxone and shock-elicited freezing in the rat.", "content": "The freezing behavior of the rat that occurs following painful electric shock was found to increase when the animal was pretreated with the opiate antagonist naloxone. Freezing was a positive linear function of drug dose and shock intensity (Experiment 2). Naloxone pretreatment enhanced freezing only when the animal was given two or three shocks but did not affect freezing when the animal was given only one shock or not shocked at all (Experiments 3, 4, and 5). Naloxone must be present during shock, nor just during the observation period, in order to increase freezing (Experiment 6). These results suggest that when an animal is shocked, it releases endogenous analgesics (endorphins) that make a subsequent shock less aversive. Naloxone, by blocking the endorphin system, makes the shock more aversive than it would normally be.", "contents": "Naloxone and shock-elicited freezing in the rat. The freezing behavior of the rat that occurs following painful electric shock was found to increase when the animal was pretreated with the opiate antagonist naloxone. Freezing was a positive linear function of drug dose and shock intensity (Experiment 2). Naloxone pretreatment enhanced freezing only when the animal was given two or three shocks but did not affect freezing when the animal was given only one shock or not shocked at all (Experiments 3, 4, and 5). Naloxone must be present during shock, nor just during the observation period, in order to increase freezing (Experiment 6). These results suggest that when an animal is shocked, it releases endogenous analgesics (endorphins) that make a subsequent shock less aversive. Naloxone, by blocking the endorphin system, makes the shock more aversive than it would normally be."}
{"id": "PMID:479406", "title": "Differential effects of localized lesions of n. accumbens on morphine- and amphetamine-induced locomotor hyperactivity in the C57BL/6J mouse.", "content": "Mice of the C57BL/6J strain became hyperactive to increasing doses of morphine sulfate. This response was similar to locomotor hyperactivity induced by amphetamine. Lesions and chemical blockade of posterior n. accumbens abolished amphetamine-induced hyperactivity and reduced but did not abolish the morphine response. These experiments demonstrate that the response to the two drugs is mediated by overlapping but noncongruent neural systems.", "contents": "Differential effects of localized lesions of n. accumbens on morphine- and amphetamine-induced locomotor hyperactivity in the C57BL/6J mouse. Mice of the C57BL/6J strain became hyperactive to increasing doses of morphine sulfate. This response was similar to locomotor hyperactivity induced by amphetamine. Lesions and chemical blockade of posterior n. accumbens abolished amphetamine-induced hyperactivity and reduced but did not abolish the morphine response. These experiments demonstrate that the response to the two drugs is mediated by overlapping but noncongruent neural systems."}
{"id": "PMID:479407", "title": "Computed tomography and ultrasonography in the evaluation of the retroperitoneum in patients with malignant ascites.", "content": "The computed tomographic (CT) body scans of 59 patients and ultrasonograms of 31 patients, all with malignant disease, were reviewed to determine the effect of ascites on the ability to image the retroperitoneum. In 95% of cases evaluated by CT, the retroperitoneal structures were well seen. In 35% of the cases evaluated by ultrasonography, the retroperitoneum was completely obscured by bowel gas and partially obscured in an additional 55%. As a result of this study, it is felt that CT should be the procedure of choice when a thorough evaluation of the retroperitoneum is necessary in patients with large amounts of abdominal ascites.", "contents": "Computed tomography and ultrasonography in the evaluation of the retroperitoneum in patients with malignant ascites. The computed tomographic (CT) body scans of 59 patients and ultrasonograms of 31 patients, all with malignant disease, were reviewed to determine the effect of ascites on the ability to image the retroperitoneum. In 95% of cases evaluated by CT, the retroperitoneal structures were well seen. In 35% of the cases evaluated by ultrasonography, the retroperitoneum was completely obscured by bowel gas and partially obscured in an additional 55%. As a result of this study, it is felt that CT should be the procedure of choice when a thorough evaluation of the retroperitoneum is necessary in patients with large amounts of abdominal ascites."}
{"id": "PMID:479408", "title": "Contrast computed tomography in the diagnosis of acute cholecystitis.", "content": "Computed tomography (CT) has the ability to detect small variations in tissue density. Meglumine diatrizoate was given intravenously to four patients with clinical acute cholecystitis as part of the CT scanning procedure. The enhancement of the thick gallbladder wall, together with an enlarged gallbladder and the presence of stones, confirmed the diagnosis of acute cholecystitis. Our present experience indicates that, after contrast medium administration, CT has a distinct place in the assessment of acute inflammation of the gallbladder.", "contents": "Contrast computed tomography in the diagnosis of acute cholecystitis. Computed tomography (CT) has the ability to detect small variations in tissue density. Meglumine diatrizoate was given intravenously to four patients with clinical acute cholecystitis as part of the CT scanning procedure. The enhancement of the thick gallbladder wall, together with an enlarged gallbladder and the presence of stones, confirmed the diagnosis of acute cholecystitis. Our present experience indicates that, after contrast medium administration, CT has a distinct place in the assessment of acute inflammation of the gallbladder."}
{"id": "PMID:479409", "title": "CT studies of the liver in vitro: a report on 82 cases with pathological correlation.", "content": "Eighty-two liver specimens were studied by both computed tomography (CT) and pathological examination with serial sectioning. In vitro CT studies showed low density structures within 57 normal livers to be due to portal and hepatic vessels but not to bile ducts. A liver with a minimum of 70% fatty infiltration could be diagnosed correctly on CT. Concerning the presence or absence of circumscribed liver disease, sensitivity was 80% and specificity was 84%. With respect to the total number of space occupying liver lesions (n = 364 in 18 specimens), sensitivity was 72%. The smallest metastases detectable on CT were 0.5 cm in diameter, but CT disclosed focal lesions of this small size in only 15% of the cases. In contrast to previously reported in vivo CT studies, metastases were often found to be of higher density than normal liver tissue in vitro.", "contents": "CT studies of the liver in vitro: a report on 82 cases with pathological correlation. Eighty-two liver specimens were studied by both computed tomography (CT) and pathological examination with serial sectioning. In vitro CT studies showed low density structures within 57 normal livers to be due to portal and hepatic vessels but not to bile ducts. A liver with a minimum of 70% fatty infiltration could be diagnosed correctly on CT. Concerning the presence or absence of circumscribed liver disease, sensitivity was 80% and specificity was 84%. With respect to the total number of space occupying liver lesions (n = 364 in 18 specimens), sensitivity was 72%. The smallest metastases detectable on CT were 0.5 cm in diameter, but CT disclosed focal lesions of this small size in only 15% of the cases. In contrast to previously reported in vivo CT studies, metastases were often found to be of higher density than normal liver tissue in vitro."}
{"id": "PMID:479410", "title": "Pectus excavatum deformities simulating mediastinal masses.", "content": "Three patients with various pectus excavatum deformities in whom radiographic findings suggested hilar and mediastinal masses are presented. Computed tomography of the chest yielded valuable diagnostic information and made it possible to exclude the existence of these masses. Compression deformity of the heart and great vessels by the pectus deformity is the most likely explanation for these findings.", "contents": "Pectus excavatum deformities simulating mediastinal masses. Three patients with various pectus excavatum deformities in whom radiographic findings suggested hilar and mediastinal masses are presented. Computed tomography of the chest yielded valuable diagnostic information and made it possible to exclude the existence of these masses. Compression deformity of the heart and great vessels by the pectus deformity is the most likely explanation for these findings."}
{"id": "PMID:479411", "title": "Demonstration of the pericardium and pericardial effusion by computed tomography.", "content": "Characteristic computed tomographic findings were present in two patients with confirmed pericardial effusion. A review of 30 chest scans without evidence of heart disease showed that the pericardium can be seen in the majority of cases.", "contents": "Demonstration of the pericardium and pericardial effusion by computed tomography. Characteristic computed tomographic findings were present in two patients with confirmed pericardial effusion. A review of 30 chest scans without evidence of heart disease showed that the pericardium can be seen in the majority of cases."}
{"id": "PMID:479412", "title": "Computed tomographic and angiographic demonstration of gastroduodenal artery pseudoaneurysm in a pancreatic pseudocyst.", "content": "The complementary use of computed tomography and angiography in two patients with bleeding gastroduodenal artery pseudoaneurysm associated with pancreatic pseudocyst is presented.", "contents": "Computed tomographic and angiographic demonstration of gastroduodenal artery pseudoaneurysm in a pancreatic pseudocyst. The complementary use of computed tomography and angiography in two patients with bleeding gastroduodenal artery pseudoaneurysm associated with pancreatic pseudocyst is presented."}
{"id": "PMID:479413", "title": "Computed tomography of the gastrointestinal tract: lesion recognition and pitfalls.", "content": "Abnormalities in the gastrointestinal tract will be found on computed tomography (CT) scans performed for other indications if they are carefully searched for. This potential will increase as CT is used earlier in the investigation of suspected abnormalities of the pancreas. retroperitoneum, and liver. Additionally, CT may be valuable in assessing the extent of a known primary lesion of the alimentary tract.", "contents": "Computed tomography of the gastrointestinal tract: lesion recognition and pitfalls. Abnormalities in the gastrointestinal tract will be found on computed tomography (CT) scans performed for other indications if they are carefully searched for. This potential will increase as CT is used earlier in the investigation of suspected abnormalities of the pancreas. retroperitoneum, and liver. Additionally, CT may be valuable in assessing the extent of a known primary lesion of the alimentary tract."}
{"id": "PMID:479414", "title": "Variation of position of the kidneys and diaphragm in patients undergoing repeated suspension of respiration.", "content": "The position of the upper poles of the kidneys in relation to the 12th thoracic vertebra was evaluated on each of three nephrograms of 32 cooperative patients undergoing excretory urography during evaluation for hypertension. Mean change in kidney position relative to vertebral position between nephrograms varied from a mean 4.9 mm on films taken during inspiration to 7.7 mm on films obtained in expiration. Diaphragmatic positional changes could be evaluated in 12 patients; mean change in diaphragmatic position be tween nephrogram exposures was 8.0 mm. Considerable variation in diaphragm position does occur during successive breath-holding attempts for cross-sectional imaging.", "contents": "Variation of position of the kidneys and diaphragm in patients undergoing repeated suspension of respiration. The position of the upper poles of the kidneys in relation to the 12th thoracic vertebra was evaluated on each of three nephrograms of 32 cooperative patients undergoing excretory urography during evaluation for hypertension. Mean change in kidney position relative to vertebral position between nephrograms varied from a mean 4.9 mm on films taken during inspiration to 7.7 mm on films obtained in expiration. Diaphragmatic positional changes could be evaluated in 12 patients; mean change in diaphragmatic position be tween nephrogram exposures was 8.0 mm. Considerable variation in diaphragm position does occur during successive breath-holding attempts for cross-sectional imaging."}
{"id": "PMID:479415", "title": "Applications of perfluorinated compounds as contrast agents in computed tomography.", "content": "Perfluorohexylbromide, a nonionic and biologically inert radiodense compound, was evaluated as a potential contrast agent. Pulmonary and gastrointestinal administration of the neat liquid form was tested in dogs in conjunction with computed tomographic body scans. Contrast enhancement was demonstrable in one organ, the spleen, within 6 hr of gastrointestinal administration of perfluorohexylbromide.", "contents": "Applications of perfluorinated compounds as contrast agents in computed tomography. Perfluorohexylbromide, a nonionic and biologically inert radiodense compound, was evaluated as a potential contrast agent. Pulmonary and gastrointestinal administration of the neat liquid form was tested in dogs in conjunction with computed tomographic body scans. Contrast enhancement was demonstrable in one organ, the spleen, within 6 hr of gastrointestinal administration of perfluorohexylbromide."}
{"id": "PMID:479416", "title": "Hyperperfusion and enhancement in dynamic computed tomography of ischemic stroke patients.", "content": "The passage of contrast medium was observed using serial computed tomography (CT) in 24 stroke patients. Density--time profiles of various brain regions were plotted. In normal brain tissue, X-ray attenuation showed a maximum increase during the arterial phase (16.4 +/- 11.0%) and was 2.8 +/- 2.2% above control during stable distribution. In hypoperfusion, increase in attenuation was always below 10% in the arterial phase, while hyperperfusion was characterized by an attenuation increase of 25 to 70%. Enhancement was defined by a density increase of 16.8 +/- 14.8% and a tissue/blood ratio between 7 and 60%. An attempt was made to establish a relationship between the serial CT pattern and the prognosis. Enhancement tended to indicate severe morphological changes followed by permanent neurological deficit, whereas hyperperfusion was generally an indicator of probably recovery.", "contents": "Hyperperfusion and enhancement in dynamic computed tomography of ischemic stroke patients. The passage of contrast medium was observed using serial computed tomography (CT) in 24 stroke patients. Density--time profiles of various brain regions were plotted. In normal brain tissue, X-ray attenuation showed a maximum increase during the arterial phase (16.4 +/- 11.0%) and was 2.8 +/- 2.2% above control during stable distribution. In hypoperfusion, increase in attenuation was always below 10% in the arterial phase, while hyperperfusion was characterized by an attenuation increase of 25 to 70%. Enhancement was defined by a density increase of 16.8 +/- 14.8% and a tissue/blood ratio between 7 and 60%. An attempt was made to establish a relationship between the serial CT pattern and the prognosis. Enhancement tended to indicate severe morphological changes followed by permanent neurological deficit, whereas hyperperfusion was generally an indicator of probably recovery."}
{"id": "PMID:479417", "title": "Fluid--fluid level in cystic cerebral metastatic melanoma.", "content": "Two cases of cystic metastatic melanoma are presented. The only reliable computed tomography sign of a true cystic lesion is the presence of an interface, such as between blood and tumor fluid. Differentiation of cystic metastatic melanoma from other cystic lesions of the brain is discussed.", "contents": "Fluid--fluid level in cystic cerebral metastatic melanoma. Two cases of cystic metastatic melanoma are presented. The only reliable computed tomography sign of a true cystic lesion is the presence of an interface, such as between blood and tumor fluid. Differentiation of cystic metastatic melanoma from other cystic lesions of the brain is discussed."}
{"id": "PMID:479418", "title": "Computed tomography of spinal fractures.", "content": "Computed tomography (CT) has aided significantly in the diagnosis and management of spinal fractures. The examination is easy, relatively quick, and avoids potentially harmful manipulation. The diagnostic advantages of CT include precise anatomic delineation and indication of extent of fractures, assessment of spinal stenosis, and demonstration of associated peraspinal lesions.", "contents": "Computed tomography of spinal fractures. Computed tomography (CT) has aided significantly in the diagnosis and management of spinal fractures. The examination is easy, relatively quick, and avoids potentially harmful manipulation. The diagnostic advantages of CT include precise anatomic delineation and indication of extent of fractures, assessment of spinal stenosis, and demonstration of associated peraspinal lesions."}
{"id": "PMID:479419", "title": "Blurring in tomograms made with x-ray beams of finite width.", "content": "Tomographic reconstruction has ordinarily assumed that the measurement data can be regarded as line integrals, but the finite width of the X-ray beam invalidates this assumption. The data can however be expressed in the form of integrals over a strip rather than a line. The strip integral kernel is calculated allowing for extended source and detector, as well as for nonuniform photon emission and detector sensitivity. Strip eccentricity, which occurs in practice, is also taken into account. Even if the measurement data were to cover all scanning angles, there would be imperfect reconstruction expressible as a space-variant point spread function deducible from the strip integral kernel. To deal with this it is convenient to introduce the concepts of generalized projection and generalized Radon transform. Point-spread functions are given for cases involving piecewise-uniform symmetrical source distributions and uniform detectors.", "contents": "Blurring in tomograms made with x-ray beams of finite width. Tomographic reconstruction has ordinarily assumed that the measurement data can be regarded as line integrals, but the finite width of the X-ray beam invalidates this assumption. The data can however be expressed in the form of integrals over a strip rather than a line. The strip integral kernel is calculated allowing for extended source and detector, as well as for nonuniform photon emission and detector sensitivity. Strip eccentricity, which occurs in practice, is also taken into account. Even if the measurement data were to cover all scanning angles, there would be imperfect reconstruction expressible as a space-variant point spread function deducible from the strip integral kernel. To deal with this it is convenient to introduce the concepts of generalized projection and generalized Radon transform. Point-spread functions are given for cases involving piecewise-uniform symmetrical source distributions and uniform detectors."}
{"id": "PMID:479420", "title": "Erosion of the scapula by a benign lipoma: computed tomography diagnosis.", "content": "A lipoma eroding the scapula is diagnosed by computed tomography.", "contents": "Erosion of the scapula by a benign lipoma: computed tomography diagnosis. A lipoma eroding the scapula is diagnosed by computed tomography."}
{"id": "PMID:479421", "title": "Diagnosis of retro-esophageal right aortic arch by computed tomography.", "content": "A case of retro-esophageal right aortic arch was clearly demonstrated by computed tomography and confirmed by arch aortography.", "contents": "Diagnosis of retro-esophageal right aortic arch by computed tomography. A case of retro-esophageal right aortic arch was clearly demonstrated by computed tomography and confirmed by arch aortography."}
{"id": "PMID:479422", "title": "Diagnosis of retroperitoneal hemorrhage by serial computed tomography.", "content": "A case of retroperitoneal hematoma is presented in which the characteristic density changes of the hematoma as seen on serial computed tomography permitted an accurate preoperative diagnosis.", "contents": "Diagnosis of retroperitoneal hemorrhage by serial computed tomography. A case of retroperitoneal hematoma is presented in which the characteristic density changes of the hematoma as seen on serial computed tomography permitted an accurate preoperative diagnosis."}
{"id": "PMID:479423", "title": "Aberrant thyroid gland demonstrated by computed tomography.", "content": "Computed tomography (CT) demonstrated high attenuation values consistent with thyroid gland in scans of a patient with tumors in the base of the tongue and anterior median neck. Radioiodine scintigraphy revealed positive uptake in the same areas. This is an indication that CT attenuation values may be useful in the diagnosis of aberrant thyroid gland.", "contents": "Aberrant thyroid gland demonstrated by computed tomography. Computed tomography (CT) demonstrated high attenuation values consistent with thyroid gland in scans of a patient with tumors in the base of the tongue and anterior median neck. Radioiodine scintigraphy revealed positive uptake in the same areas. This is an indication that CT attenuation values may be useful in the diagnosis of aberrant thyroid gland."}
{"id": "PMID:479424", "title": "Acute isodense epidural hematoma on computed tomography.", "content": "Computed tomography (CT) has proven to be invaluable in the diagnosis of craniocerebral injuries. Acute epidural hematomas have been described as having a characteristic appearance on CT of a lenticular shaped area of blood density most commonly situated over the cerebral convexity. This case demonstrates an acute epidural hematoma that is essentially isodense with brain tissue.", "contents": "Acute isodense epidural hematoma on computed tomography. Computed tomography (CT) has proven to be invaluable in the diagnosis of craniocerebral injuries. Acute epidural hematomas have been described as having a characteristic appearance on CT of a lenticular shaped area of blood density most commonly situated over the cerebral convexity. This case demonstrates an acute epidural hematoma that is essentially isodense with brain tissue."}
{"id": "PMID:479425", "title": "Scalp markers for precise craniotomy siting, using computed tomography.", "content": "A rapid, simple, and inexpensive computed tomographic technique has been developed for marking the scalp and lateral skull radiograph of patients with small cerebral convexity lesions. The more precise localization provided thereby facilitates planning and performance of surgery.", "contents": "Scalp markers for precise craniotomy siting, using computed tomography. A rapid, simple, and inexpensive computed tomographic technique has been developed for marking the scalp and lateral skull radiograph of patients with small cerebral convexity lesions. The more precise localization provided thereby facilitates planning and performance of surgery."}
{"id": "PMID:479426", "title": "Support and bolusing device for scanning small objects.", "content": "A versatile bolusing device made of polystyrene is described for scanning small objects in currently available \"air gap\" computed tomography scanners. The device, consisting of three concentric rings fitted inside one another, allows for artifact-free and reproducible imaging.", "contents": "Support and bolusing device for scanning small objects. A versatile bolusing device made of polystyrene is described for scanning small objects in currently available \"air gap\" computed tomography scanners. The device, consisting of three concentric rings fitted inside one another, allows for artifact-free and reproducible imaging."}
{"id": "PMID:479427", "title": "Direct sagittal computed tomography of the brain.", "content": "The first results of a simple method for obtaining direct sagittal computed tomography scans of the brain with a Pfizer 200 FS scanner are presented with a brief discussion of the advantages and applications of this technique.", "contents": "Direct sagittal computed tomography of the brain. The first results of a simple method for obtaining direct sagittal computed tomography scans of the brain with a Pfizer 200 FS scanner are presented with a brief discussion of the advantages and applications of this technique."}
{"id": "PMID:479428", "title": "Basal cell carcinoma--signet ring type. Ultrastructural study.", "content": "Light microscopic examination of a basalioma (basal cell carcinoma) revealed unusual keratinizing cells resembling signet ring cells with pink cytoplasmic inclusions. Ultrastructurally the inclusions consisted of filamentous masses encircled by abundant tonofilaments giving a striking picture of abnormal individual tumor cell keratinization.", "contents": "Basal cell carcinoma--signet ring type. Ultrastructural study. Light microscopic examination of a basalioma (basal cell carcinoma) revealed unusual keratinizing cells resembling signet ring cells with pink cytoplasmic inclusions. Ultrastructurally the inclusions consisted of filamentous masses encircled by abundant tonofilaments giving a striking picture of abnormal individual tumor cell keratinization."}
{"id": "PMID:479429", "title": "Giant cells in male pattern alopecia: a histologic marker and pathogenetic clue.", "content": "Twenty-one of 65 (31%) biopsy specimens from patients with male pattern alopecia demonstrated the presence of individually displaced multinucleate giant cells without a significant concomitant cellular infiltrate. This finding was absent in a diverse group of other forms of alopecia. Whether individually displaced multinucleate cells are a useful histologic marker for diagnosing early stages of androgenetic alopecia remains to be determined.", "contents": "Giant cells in male pattern alopecia: a histologic marker and pathogenetic clue. Twenty-one of 65 (31%) biopsy specimens from patients with male pattern alopecia demonstrated the presence of individually displaced multinucleate giant cells without a significant concomitant cellular infiltrate. This finding was absent in a diverse group of other forms of alopecia. Whether individually displaced multinucleate cells are a useful histologic marker for diagnosing early stages of androgenetic alopecia remains to be determined."}
{"id": "PMID:479430", "title": "Pagetoid reticulosis (Woringer-Kolopp disease).", "content": "Two patients with solitary lesions of pagetoid reticulosis are presented. A third case with disseminated lesions and similar microscopic detail is also reported. Lighg microscopic findings include an abnornal cell infiltrate confined to the epidermis and distributed diffusely in a pagetoid pattern. In ultrathin sections, the abnormal cells are seen to have an abundant cytoplasm with numerous organelles and a convoluted nucleus, often with a prominent nucleolus. The nature of the abnormal cells is discussed. The infiltrate is differentiated from that of mycosis fungoides.", "contents": "Pagetoid reticulosis (Woringer-Kolopp disease). Two patients with solitary lesions of pagetoid reticulosis are presented. A third case with disseminated lesions and similar microscopic detail is also reported. Lighg microscopic findings include an abnornal cell infiltrate confined to the epidermis and distributed diffusely in a pagetoid pattern. In ultrathin sections, the abnormal cells are seen to have an abundant cytoplasm with numerous organelles and a convoluted nucleus, often with a prominent nucleolus. The nature of the abnormal cells is discussed. The infiltrate is differentiated from that of mycosis fungoides."}
{"id": "PMID:479431", "title": "Biopsies of the Janeway lesion of infective endocarditis.", "content": "Janeway lesions in three patients with infective endocarditis caused by Staphylococcus aureus were biopsied. Two taken before therapy, showed evidence of suppuration and microabscesses. The other lesion which developed while the patient was on appropriate therapy, showed minimal inflammatory reaction.", "contents": "Biopsies of the Janeway lesion of infective endocarditis. Janeway lesions in three patients with infective endocarditis caused by Staphylococcus aureus were biopsied. Two taken before therapy, showed evidence of suppuration and microabscesses. The other lesion which developed while the patient was on appropriate therapy, showed minimal inflammatory reaction."}
{"id": "PMID:479432", "title": "Solitary actinic porokeratosis.", "content": "An 83-year-old man experienced abrupt onset of a solitary lesion of porokeratosis on his bald, actinically damaged scalp. The features in this case are unusual and represent another point in the broad spectrum of lesions characterized by the cornoid lamella of porokeratosis.", "contents": "Solitary actinic porokeratosis. An 83-year-old man experienced abrupt onset of a solitary lesion of porokeratosis on his bald, actinically damaged scalp. The features in this case are unusual and represent another point in the broad spectrum of lesions characterized by the cornoid lamella of porokeratosis."}
{"id": "PMID:479433", "title": "Pityriasis rosea in a patient with epidermolysis bullosa dystrophica.", "content": "A patient with clinical and histologic features of epidermolysis bullosa dystrophica-recessive (EBD-R) developed superimposed clinical lesions of pityriasis rosea (PR). Electron microscopy showed distinct \"blebbing\" of basal cells at the basement membrane zone in clinically normal skin of a non-predilected area, as well as in scarred skin from a predilected area and a lesion of PR. An increased susceptibility of the basement membrane zone to proteolytic enzymes is suggested as a possible hypothesis to explain these observations.", "contents": "Pityriasis rosea in a patient with epidermolysis bullosa dystrophica. A patient with clinical and histologic features of epidermolysis bullosa dystrophica-recessive (EBD-R) developed superimposed clinical lesions of pityriasis rosea (PR). Electron microscopy showed distinct \"blebbing\" of basal cells at the basement membrane zone in clinically normal skin of a non-predilected area, as well as in scarred skin from a predilected area and a lesion of PR. An increased susceptibility of the basement membrane zone to proteolytic enzymes is suggested as a possible hypothesis to explain these observations."}
{"id": "PMID:479435", "title": "Multiple keratoacanthomas and squamous cell carcinomas occurring at psoriatic treatment sites.", "content": "A report on four patients with psoriasis who have developed multiple keratoacanthomas and squamous cell carcinoma at the treatment sites is presented. Two other reports have appeared in the literature which link the development of keratoacanthoma with psoriasis and pustular psoriasis. Although a number of etiologic factors have been suspected for the development of these new growths, this presentation will further assess the specific roles that treatment measures may have played in these cases. Furthermore, the significance of impaired cellular immune defense mechanisms will be discussed.", "contents": "Multiple keratoacanthomas and squamous cell carcinomas occurring at psoriatic treatment sites. A report on four patients with psoriasis who have developed multiple keratoacanthomas and squamous cell carcinoma at the treatment sites is presented. Two other reports have appeared in the literature which link the development of keratoacanthoma with psoriasis and pustular psoriasis. Although a number of etiologic factors have been suspected for the development of these new growths, this presentation will further assess the specific roles that treatment measures may have played in these cases. Furthermore, the significance of impaired cellular immune defense mechanisms will be discussed."}
{"id": "PMID:479436", "title": "A tridimensional view of intradermal nevi as revealed by scanning electron microscopy.", "content": "When scanning electron microscopy (SEM) was applied to the study of intradermal nevi, interesting tridimensional features were recognized. The free surface of the lesions showed ruffled keratinized cells. \"Normal\" hairs as well as \"corkscrew\" hairs emerged from the follicular openings. Nevus cells were either round or elongated and surrounded by connective tissue fibers.", "contents": "A tridimensional view of intradermal nevi as revealed by scanning electron microscopy. When scanning electron microscopy (SEM) was applied to the study of intradermal nevi, interesting tridimensional features were recognized. The free surface of the lesions showed ruffled keratinized cells. \"Normal\" hairs as well as \"corkscrew\" hairs emerged from the follicular openings. Nevus cells were either round or elongated and surrounded by connective tissue fibers."}
{"id": "PMID:479437", "title": "Clinical and pathological correlation of malignant melanoma.", "content": "We have attempted to review virtually all forms of cutaneous and mucocutaneous melanomas. Superficial spreading, lentigo maligna and nodular melanomas have been more thoroughly investigated and documented in previous studies. Lentigo maligna melanoma appears to have a longer duration and better prognosis than SSM or NM. The overall prognosis probably correlates better with the anatomic level and thickness of invasion than with type (Clark et al. 1975, Breslow 1970, 1975). It appears that certain pitfalls exist in either method of assessing prognosis, and it is recommended that both methods be applied in evaluating a malignant melanocytic lesion when feasible. With regard to in situ melanoma or Level I melanoma, it is our experience that such lesions can achieve a 100% cure rate when completely excised. Hence, we prefer to call such lesions severely atypical melanocytic hyperplasia, and thus avoid labeling these patients with a malignant diagnosis. The most difficult histologic challenge in diagnosing a lesion of malignant melanoma is the Spitz nevus. The pathologist should never be biased by the age of the patient, for a serious mistake can arise. We have seen a case of nodular melanoma in a 13-year-old girl diagnosed as Spitz nevus only to be followed by a lymph node metastasis years later. Other examples of histologic differential diagnoses of malignant melanomas include, for example, halo nevus, soft tissue sarcoma, squamous cell carcinoma with spindle cell proliferation, Paget's disease of metastatic carcinoma, (for example, from the breast). Therefore, the approach to the diagnosis of malignant melanoma necessitates an evaluation of both clinical and pathological features. Histologic study must encompass both the pattern of growth and cellular cytologic detail for successful interpretation.", "contents": "Clinical and pathological correlation of malignant melanoma. We have attempted to review virtually all forms of cutaneous and mucocutaneous melanomas. Superficial spreading, lentigo maligna and nodular melanomas have been more thoroughly investigated and documented in previous studies. Lentigo maligna melanoma appears to have a longer duration and better prognosis than SSM or NM. The overall prognosis probably correlates better with the anatomic level and thickness of invasion than with type (Clark et al. 1975, Breslow 1970, 1975). It appears that certain pitfalls exist in either method of assessing prognosis, and it is recommended that both methods be applied in evaluating a malignant melanocytic lesion when feasible. With regard to in situ melanoma or Level I melanoma, it is our experience that such lesions can achieve a 100% cure rate when completely excised. Hence, we prefer to call such lesions severely atypical melanocytic hyperplasia, and thus avoid labeling these patients with a malignant diagnosis. The most difficult histologic challenge in diagnosing a lesion of malignant melanoma is the Spitz nevus. The pathologist should never be biased by the age of the patient, for a serious mistake can arise. We have seen a case of nodular melanoma in a 13-year-old girl diagnosed as Spitz nevus only to be followed by a lymph node metastasis years later. Other examples of histologic differential diagnoses of malignant melanomas include, for example, halo nevus, soft tissue sarcoma, squamous cell carcinoma with spindle cell proliferation, Paget's disease of metastatic carcinoma, (for example, from the breast). Therefore, the approach to the diagnosis of malignant melanoma necessitates an evaluation of both clinical and pathological features. Histologic study must encompass both the pattern of growth and cellular cytologic detail for successful interpretation."}
{"id": "PMID:479438", "title": "Immune derangement in patients with malignant melanoma.", "content": "The interaction between immune system and growing tumor can be expressed differently at different stages of the disease. This presentation covers three facets of these reactions in melanoma patients. A. The Primary Tumor. Time-lapse cinematography, with an analysis of lymphocyte movement demonstrated positive and negative chemotaxis against tumor tissues which correlated with their histological presence or absence within the primary tumor. B. The Regional Lymph Nodes. Histological examination of regional lymph nodes showed an increase in germinal center activity and B cell number, with a decrease in sinus histiocytosis and monocyte count as the tumor progressively invaded the node. This correlated with the elution studies, wherein the antimembrane antibody decreased and the anti-cytoplasmic antibody increased during the same period of progression. C. Humoral Immunity and Metastasis. Clinical metastasis heralded the decrease of anti-membrane antibodies with a rise in anti-immunoglobulins, especially anti-idiotypic antibodies and immune complexes containing tumor-directed antibody and either antigen or anti-immunoglobulin. This triad of anti-immunoglobulin, immune complexes and anergy as seen in other diseases with persistent antigenic stimulation, results in abnormal regulation and derangement.", "contents": "Immune derangement in patients with malignant melanoma. The interaction between immune system and growing tumor can be expressed differently at different stages of the disease. This presentation covers three facets of these reactions in melanoma patients. A. The Primary Tumor. Time-lapse cinematography, with an analysis of lymphocyte movement demonstrated positive and negative chemotaxis against tumor tissues which correlated with their histological presence or absence within the primary tumor. B. The Regional Lymph Nodes. Histological examination of regional lymph nodes showed an increase in germinal center activity and B cell number, with a decrease in sinus histiocytosis and monocyte count as the tumor progressively invaded the node. This correlated with the elution studies, wherein the antimembrane antibody decreased and the anti-cytoplasmic antibody increased during the same period of progression. C. Humoral Immunity and Metastasis. Clinical metastasis heralded the decrease of anti-membrane antibodies with a rise in anti-immunoglobulins, especially anti-idiotypic antibodies and immune complexes containing tumor-directed antibody and either antigen or anti-immunoglobulin. This triad of anti-immunoglobulin, immune complexes and anergy as seen in other diseases with persistent antigenic stimulation, results in abnormal regulation and derangement."}
{"id": "PMID:479439", "title": "Prognostic factors in the treatment of cutaneous melanoma.", "content": "The incidence of metastasis in cutaneous melanoma is proportional to maximal tumor thickness. The relationship is linear for extremity melanoma but not for tumors from all body sites, probably due to intrinsic differences in tumors from different sites. The level of invasion of the tumor is an indirect measure of tumor thickness and is not as accurate in predicting metastases because of the marked variation in thickness within each level. The implications of these observations for the treatment of melanoma are discussed.", "contents": "Prognostic factors in the treatment of cutaneous melanoma. The incidence of metastasis in cutaneous melanoma is proportional to maximal tumor thickness. The relationship is linear for extremity melanoma but not for tumors from all body sites, probably due to intrinsic differences in tumors from different sites. The level of invasion of the tumor is an indirect measure of tumor thickness and is not as accurate in predicting metastases because of the marked variation in thickness within each level. The implications of these observations for the treatment of melanoma are discussed."}
{"id": "PMID:479440", "title": "Surgical management of melanoma.", "content": "Traditionally, melanoma has been considered by surgeons to be a highly lethal malignancy, always requiring a radical surgical approach for optimal chance of cure. Yet melanoma patients who are now being referred for treatment frequently have small early lesions, and surgeons should develop a flexible attitude about management, limiting the size of their excisions and deferring node dissections in many early cases. An aggressive, radical approach remains appropriate for large lesions, progressively enlarging nodes and locally recurrent but not disseminated disease.", "contents": "Surgical management of melanoma. Traditionally, melanoma has been considered by surgeons to be a highly lethal malignancy, always requiring a radical surgical approach for optimal chance of cure. Yet melanoma patients who are now being referred for treatment frequently have small early lesions, and surgeons should develop a flexible attitude about management, limiting the size of their excisions and deferring node dissections in many early cases. An aggressive, radical approach remains appropriate for large lesions, progressively enlarging nodes and locally recurrent but not disseminated disease."}
{"id": "PMID:479446", "title": "Aggressive biologic behavior of basal- and squamous-cell cancers in patients with chronic lymphocytic leukemia or chronic lymphocytic lymphoma.", "content": "Three basal- and four squamous-cell carcinomas in seven patients with chronic lymphocytic leukemia or chronic lymphocytic lymphoma recurred repeatedly after conventional treatment, and grew to large sizes. The squamous-cell carcinomas metastasized in all four of the patients so afflicted. Absolute numbers of circulating T lymphocytes were normal in the seven patients, but they had cutaneous anergy to intradermal tests with common antigens and to dinitrochlorobenzene. The following recommendations for management of cutaneous carcinomas in patients with malignant lymphomatoses are made: 1) closer surveillance than for patients with cutaneous cancers but without malignant lymphomatoses, 2) early treatment of actinic keratoses to prevent possible transformation to malignancy, and 3) microscopically controlled excision of basal- or squamous-cell carcinomas larger than 1 cm in diameter.", "contents": "Aggressive biologic behavior of basal- and squamous-cell cancers in patients with chronic lymphocytic leukemia or chronic lymphocytic lymphoma. Three basal- and four squamous-cell carcinomas in seven patients with chronic lymphocytic leukemia or chronic lymphocytic lymphoma recurred repeatedly after conventional treatment, and grew to large sizes. The squamous-cell carcinomas metastasized in all four of the patients so afflicted. Absolute numbers of circulating T lymphocytes were normal in the seven patients, but they had cutaneous anergy to intradermal tests with common antigens and to dinitrochlorobenzene. The following recommendations for management of cutaneous carcinomas in patients with malignant lymphomatoses are made: 1) closer surveillance than for patients with cutaneous cancers but without malignant lymphomatoses, 2) early treatment of actinic keratoses to prevent possible transformation to malignancy, and 3) microscopically controlled excision of basal- or squamous-cell carcinomas larger than 1 cm in diameter."}
{"id": "PMID:479447", "title": "Wound healing: a review. III. Nutritional factors affecting wound healing.", "content": "Wound healing proceeds more efficiently and quickly in well-nourished individuals who are in good general health at the time that they have to undergo surgery. Per contra, individuals who are malnourished and chronically ill heal less well and are in general at greater risk of complications during and after surgery. For the latter, elective surgery may be deferred until nutritional improvement is attained, but for emergency or urgent operations, institution of measures promoting good nutrition has to be concurrent with necessitous surgery and continued postoperatively. In this paper, the importance to wound healing of proteins, carbohydrates, fats, vitamins, and trace elements and minerals is reviewed.", "contents": "Wound healing: a review. III. Nutritional factors affecting wound healing. Wound healing proceeds more efficiently and quickly in well-nourished individuals who are in good general health at the time that they have to undergo surgery. Per contra, individuals who are malnourished and chronically ill heal less well and are in general at greater risk of complications during and after surgery. For the latter, elective surgery may be deferred until nutritional improvement is attained, but for emergency or urgent operations, institution of measures promoting good nutrition has to be concurrent with necessitous surgery and continued postoperatively. In this paper, the importance to wound healing of proteins, carbohydrates, fats, vitamins, and trace elements and minerals is reviewed."}
{"id": "PMID:479448", "title": "Special techniques of hair transplantation by fusiform grafts and flaps of many types.", "content": "There are more and better ways of transplanting scalp hair than merely by punch grafts. Techniques of transplantation by fusiform grafts and flaps are briefly described, advantages are stated, and results are illustrated.", "contents": "Special techniques of hair transplantation by fusiform grafts and flaps of many types. There are more and better ways of transplanting scalp hair than merely by punch grafts. Techniques of transplantation by fusiform grafts and flaps are briefly described, advantages are stated, and results are illustrated."}
{"id": "PMID:479450", "title": "Basal-cell carcinoma in the youthful: report of a case in a young woman.", "content": "A rare instance of the spontaneous development of a basal-cell carcinoma in front of the tragus in a young person of 19 years and its management are described.", "contents": "Basal-cell carcinoma in the youthful: report of a case in a young woman. A rare instance of the spontaneous development of a basal-cell carcinoma in front of the tragus in a young person of 19 years and its management are described."}
{"id": "PMID:479474", "title": "Presidential address. The advancement of the knowledge and practice of allergy.", "content": "State and local allergy societies have appeared in response to pressures created by progressive specialization, increase in knowledge, and need for cost control within the field of allergy and clinical immunology. They provide a mechanism whereby allergists and immunologists with many different interests and functions can work in an integrated and cooperative manner to provide, in a cost-effective fashion, adequate patient care, teaching, and research in allergy and immunology for the geographic area. Maximum effectiveness requires close liaison with medical schools, state medical societies, national allergy organizations, and the local chapter of the Asthma and Allergy Foundation of America. Their structure should permit frequent close communication between members in various fields of allergy and clinical immunology, and the organization of committees that can accomplish the hard work of ensuring care for the disadvantaged patient, time and money for research, cost control, identification and rehabilitation of the poorly functioning allergists, continuing education for physician and patient, and all of the other tasks that make the practice of allergy and clinical immunology such a wonderful and absorbing way of life.", "contents": "Presidential address. The advancement of the knowledge and practice of allergy. State and local allergy societies have appeared in response to pressures created by progressive specialization, increase in knowledge, and need for cost control within the field of allergy and clinical immunology. They provide a mechanism whereby allergists and immunologists with many different interests and functions can work in an integrated and cooperative manner to provide, in a cost-effective fashion, adequate patient care, teaching, and research in allergy and immunology for the geographic area. Maximum effectiveness requires close liaison with medical schools, state medical societies, national allergy organizations, and the local chapter of the Asthma and Allergy Foundation of America. Their structure should permit frequent close communication between members in various fields of allergy and clinical immunology, and the organization of committees that can accomplish the hard work of ensuring care for the disadvantaged patient, time and money for research, cost control, identification and rehabilitation of the poorly functioning allergists, continuing education for physician and patient, and all of the other tasks that make the practice of allergy and clinical immunology such a wonderful and absorbing way of life."}
{"id": "PMID:479476", "title": "Further characterization and biologic activity of ragweed antigen--induced neutrophil chemotactic activity in man.", "content": "We have previously described the appearance in serum of increased neutrophil chemotactic activity (NCA) during bronchospasm induced by inhalation of ragweed antigen in ragweed-sensitive subjects. This NCA is non-complement derived, appears within 1 min after antigen inhalation, and is not seen after methacholine-induced bronchospasm. This article describes further characterization of this chemotactic activity and correlation with in vivo leukocytosis. NCA consistently eluted in the void volume (fraction I) after Sephadex G-150 chromatography of patient serum obtained 10 min postchallenge. Fraction I contained 94% of the NCA of postchallenge whole serum. Both postchallenge whole serum and fraction I deactivated neutrophils to autologous chemoattractants and complement-derived chemotactic factors, but not serum-independent chemotactic factors. NCA was chemotactic for neither human nor guinea pig eosinophils, nor for human mononuclear cells. A significant increase of circulating neutrophils was seen only after antigen-induced bronchospasm and correlated with the increase in NCA. Thus, NCA represents another inflammatory mediator of probable mast cell origin that may explain, at least partially, the accumulation of neutrophils observed in the peripheral blood, skin, and bronchial wall after immediate hypersensitivity reactions.", "contents": "Further characterization and biologic activity of ragweed antigen--induced neutrophil chemotactic activity in man. We have previously described the appearance in serum of increased neutrophil chemotactic activity (NCA) during bronchospasm induced by inhalation of ragweed antigen in ragweed-sensitive subjects. This NCA is non-complement derived, appears within 1 min after antigen inhalation, and is not seen after methacholine-induced bronchospasm. This article describes further characterization of this chemotactic activity and correlation with in vivo leukocytosis. NCA consistently eluted in the void volume (fraction I) after Sephadex G-150 chromatography of patient serum obtained 10 min postchallenge. Fraction I contained 94% of the NCA of postchallenge whole serum. Both postchallenge whole serum and fraction I deactivated neutrophils to autologous chemoattractants and complement-derived chemotactic factors, but not serum-independent chemotactic factors. NCA was chemotactic for neither human nor guinea pig eosinophils, nor for human mononuclear cells. A significant increase of circulating neutrophils was seen only after antigen-induced bronchospasm and correlated with the increase in NCA. Thus, NCA represents another inflammatory mediator of probable mast cell origin that may explain, at least partially, the accumulation of neutrophils observed in the peripheral blood, skin, and bronchial wall after immediate hypersensitivity reactions."}
{"id": "PMID:479477", "title": "Allergenicity and lymphocyte-stimulating property of rice protein.", "content": "Two protein fractions of rice, grain, glutelin and globulin, were prepared by dilute alkali and salt extraction, respectively. The globulin fraction was separated into G1-1, G1-2, and G1-3 fractions by Sephadex G-200 column chromatography. The allergenic activities and lymphocyte-stimulating properties of these fractions were investigated by the radioallergosorbent test (RAST) with sera from 6 individuals who showed immediate skin reaction to soluble rice extract and by 3H-thymidine incorporation tests with 5 subjects with indurated skin reaction of delayed onset. All fractions were found to be reactive with specific IgE antibody, and G1-1 and G-2 revealed lymphocyte-stimulating activity. RAST inhibition revealed considerable cross-reactivity of IgE antibody with the glutelin and globulin fractions. When the glutelin and globulin fractions were heated at 60 degrees C for 1 hr, 100 degrees C for 2 min, or 100 degrees C for 10 min, RAST activities were reduced to 40%-70% of native. On the other hand, lymphocyte-stimulating activities of the globulin fraction heated at 60 degrees C for 1 hr or 100 degrees C for 2 min were enhanced up to 6 times of native activities, while those of identically treated glutelin fractions remained unchanged.", "contents": "Allergenicity and lymphocyte-stimulating property of rice protein. Two protein fractions of rice, grain, glutelin and globulin, were prepared by dilute alkali and salt extraction, respectively. The globulin fraction was separated into G1-1, G1-2, and G1-3 fractions by Sephadex G-200 column chromatography. The allergenic activities and lymphocyte-stimulating properties of these fractions were investigated by the radioallergosorbent test (RAST) with sera from 6 individuals who showed immediate skin reaction to soluble rice extract and by 3H-thymidine incorporation tests with 5 subjects with indurated skin reaction of delayed onset. All fractions were found to be reactive with specific IgE antibody, and G1-1 and G-2 revealed lymphocyte-stimulating activity. RAST inhibition revealed considerable cross-reactivity of IgE antibody with the glutelin and globulin fractions. When the glutelin and globulin fractions were heated at 60 degrees C for 1 hr, 100 degrees C for 2 min, or 100 degrees C for 10 min, RAST activities were reduced to 40%-70% of native. On the other hand, lymphocyte-stimulating activities of the globulin fraction heated at 60 degrees C for 1 hr or 100 degrees C for 2 min were enhanced up to 6 times of native activities, while those of identically treated glutelin fractions remained unchanged."}
{"id": "PMID:479478", "title": "The seasonal variation in a population of house dust mites in a North American city.", "content": "Mattress dust collected at monthly intervals for 2 1/2 yr was examined for mites. Both Dermatophagoides pteronyssinus and D. farinae were found. There was a significant association between the presence of live mites and the relative humidity (RH): Live mites were seen only when the RH had been greater than or equal to 50% for at least part of every day during the month of collection. There was a seasonal variation in that live mites were found only in the warmer months and not in the winter. However, the peak in the mite population was consistent neither for month nor for numbers: In 1977 a slight peak in mite numbers occurred in October, while in 1978 the peak was higher and occurred in July. Use of data from previously published studies to preduct mite levels may therefore be misleading.", "contents": "The seasonal variation in a population of house dust mites in a North American city. Mattress dust collected at monthly intervals for 2 1/2 yr was examined for mites. Both Dermatophagoides pteronyssinus and D. farinae were found. There was a significant association between the presence of live mites and the relative humidity (RH): Live mites were seen only when the RH had been greater than or equal to 50% for at least part of every day during the month of collection. There was a seasonal variation in that live mites were found only in the warmer months and not in the winter. However, the peak in the mite population was consistent neither for month nor for numbers: In 1977 a slight peak in mite numbers occurred in October, while in 1978 the peak was higher and occurred in July. Use of data from previously published studies to preduct mite levels may therefore be misleading."}
{"id": "PMID:479479", "title": "Acute allergic reaction after composite pollen ingestion.", "content": "Three patients are presented who ingested a health food known as \"bee pollen\" and who experienced an immediate allergic reaction. Examination revealed that the bee pollen contained dandelion pollen, which belongs to the Compositae family as does ragweed. In vivo and in vitro studies demonstrated that the patients were sensitive to several Compositae family members, rather than to insect-derived antigens. In addition, radioallergosorbent inhibition studies confirmed the suspected cross-reactivity between the health food and Compositae pollens. This study indicates that atopic individuals may experience anaphylaxis from ingestion of antigens in certain food stuffs that cross-react with pollens to which they are sensitive.", "contents": "Acute allergic reaction after composite pollen ingestion. Three patients are presented who ingested a health food known as \"bee pollen\" and who experienced an immediate allergic reaction. Examination revealed that the bee pollen contained dandelion pollen, which belongs to the Compositae family as does ragweed. In vivo and in vitro studies demonstrated that the patients were sensitive to several Compositae family members, rather than to insect-derived antigens. In addition, radioallergosorbent inhibition studies confirmed the suspected cross-reactivity between the health food and Compositae pollens. This study indicates that atopic individuals may experience anaphylaxis from ingestion of antigens in certain food stuffs that cross-react with pollens to which they are sensitive."}
{"id": "PMID:479480", "title": "Clinical and biochemical effects of impeded androgen (oxymetholone) therapy of hereditary angioedema.", "content": "Daily therapy and alternate-day therapy with the attenuated androgen oxymetholone were compared in patients with hereditary angioedema (HAE). Fifteen of 16 patients who experienced at least monthly attacks of HAE without treatment were asymptomatic on administration of 5 mg oxymetholene daily. When 13 of the patients who had been maintained asymptomatically on 5 mg oxymetholone daily were advanced to a treatment schedule of 5 mg every other day, seven attacks occurred during a cummulative 50 mo of therapy. The adverse effects that occurred with daily oxymetholone therapy largely subsided when the patients received alternate-day therapy, while a significant mean rise in C4 protein and function occurred only on daily therapy. Statistically significant mean increases in serum levels of C1INH occurred with daily therapy and were maintained with alternate-day therapy. Clinical benefit can be obtained with a treatment program that does not produce a statistically significant rise in C4 protein or function and does not raise C1INH to the lower limit of normal. The finding that alternate-day therapy diminished the side effects of the drug while affording a substantial reduction in the incidence and severity of attacks indicates the feasibility of this therapeutic approach.", "contents": "Clinical and biochemical effects of impeded androgen (oxymetholone) therapy of hereditary angioedema. Daily therapy and alternate-day therapy with the attenuated androgen oxymetholone were compared in patients with hereditary angioedema (HAE). Fifteen of 16 patients who experienced at least monthly attacks of HAE without treatment were asymptomatic on administration of 5 mg oxymetholene daily. When 13 of the patients who had been maintained asymptomatically on 5 mg oxymetholone daily were advanced to a treatment schedule of 5 mg every other day, seven attacks occurred during a cummulative 50 mo of therapy. The adverse effects that occurred with daily oxymetholone therapy largely subsided when the patients received alternate-day therapy, while a significant mean rise in C4 protein and function occurred only on daily therapy. Statistically significant mean increases in serum levels of C1INH occurred with daily therapy and were maintained with alternate-day therapy. Clinical benefit can be obtained with a treatment program that does not produce a statistically significant rise in C4 protein or function and does not raise C1INH to the lower limit of normal. The finding that alternate-day therapy diminished the side effects of the drug while affording a substantial reduction in the incidence and severity of attacks indicates the feasibility of this therapeutic approach."}
{"id": "PMID:479481", "title": "Prostaglandin generation by human and guinea pig lung tissue: comparison of parenchymal and airway responses.", "content": "Anaphylaxis of human lung is accompanied by the synthesis of prostaglandins (PG), including PGF2 alpha and PGE. In an analysis of the tissue source of these prostaglandins, parenchymal preparations of both human and guinea pig (GP) lungs were compared. Peripheral, relatively airway-free preparations of human lung generate PGF2 alpha and PGE in response to histamine and 2-methylhistamine, on H1 agonist, but not to dimaprit, an H2 agonist. GP parenchymal preparations respond in a similar fashion. Stimulation of these same preparations with KCl or carbachol caused no increase in the synthesis of either PG. In human airway preparations all three agonists (histamine, KCl, and carbachol) caused the selective generation of PGE. However, stimulation of GP airway preparations with the agonists caused the production of both PGE and PGF2 alpha. These data indicate that (1) human and GP peripheral lung tissues respond to H1, but not H2, stimulation with the generation of PGF2 alpha and PGE; (2) these parenchymal responses are specific and may not be attributed to muscle contraction; and (3) stimulation of muscle contraction in human airway preparations results in the selective generation of PGE while GP airways produce both PGE and PGF2 alpha.", "contents": "Prostaglandin generation by human and guinea pig lung tissue: comparison of parenchymal and airway responses. Anaphylaxis of human lung is accompanied by the synthesis of prostaglandins (PG), including PGF2 alpha and PGE. In an analysis of the tissue source of these prostaglandins, parenchymal preparations of both human and guinea pig (GP) lungs were compared. Peripheral, relatively airway-free preparations of human lung generate PGF2 alpha and PGE in response to histamine and 2-methylhistamine, on H1 agonist, but not to dimaprit, an H2 agonist. GP parenchymal preparations respond in a similar fashion. Stimulation of these same preparations with KCl or carbachol caused no increase in the synthesis of either PG. In human airway preparations all three agonists (histamine, KCl, and carbachol) caused the selective generation of PGE. However, stimulation of GP airway preparations with the agonists caused the production of both PGE and PGF2 alpha. These data indicate that (1) human and GP peripheral lung tissues respond to H1, but not H2, stimulation with the generation of PGF2 alpha and PGE; (2) these parenchymal responses are specific and may not be attributed to muscle contraction; and (3) stimulation of muscle contraction in human airway preparations results in the selective generation of PGE while GP airways produce both PGE and PGF2 alpha."}
{"id": "PMID:479482", "title": "Binding of immunoglobulin and activation of complement by asbestos fibers.", "content": "Asbestos fibers adsorbed IgG from whole human serum in amounts comparable to that taken up by crystals of calcium pyrophosphate or hydroxyapatite, but less than that adsorbed by crystals of monosodium urate monohydrate. Chrysotile asbestos was much more active in binding IgG than was amphibole asbestos. The electrophoretic mobility of adsorbed IgG was a function of the surface charge of the crystal studied. Negatively charged fibers adsorbed cationic IgG, and positively charged fibers adsorbed anionic IgG. Complement activation by asbestos, as judged by electrophoretic conversion of C3, was similar for all fiber types tested. Properdin factor B was also activated in the presence of asbestos. Activation of both properdin factor B and C3 was only partially inhibited by ethylene glycol tetraacetic acid, demonstrating that activation was occurring through both the classical and alternative pathways.", "contents": "Binding of immunoglobulin and activation of complement by asbestos fibers. Asbestos fibers adsorbed IgG from whole human serum in amounts comparable to that taken up by crystals of calcium pyrophosphate or hydroxyapatite, but less than that adsorbed by crystals of monosodium urate monohydrate. Chrysotile asbestos was much more active in binding IgG than was amphibole asbestos. The electrophoretic mobility of adsorbed IgG was a function of the surface charge of the crystal studied. Negatively charged fibers adsorbed cationic IgG, and positively charged fibers adsorbed anionic IgG. Complement activation by asbestos, as judged by electrophoretic conversion of C3, was similar for all fiber types tested. Properdin factor B was also activated in the presence of asbestos. Activation of both properdin factor B and C3 was only partially inhibited by ethylene glycol tetraacetic acid, demonstrating that activation was occurring through both the classical and alternative pathways."}
{"id": "PMID:479483", "title": "Effect of unsaturated fats and cholesterol on serum and fecal lipids. A study of healthy middle-aged men.", "content": "Modification of the Western diet, with an intake of 2,500 kcal per day and a decrease in cholesterol and fat intake to 33 per cent of calories with a P:S of 0.40, failed to alter serum lipids significantly. Evidence suggested that increasing the daily intake of cholesterol resulted in deposition in the body tissues. However, when the fatty acid composition and the P:S ratio of dietary fat were adjusted, lower serum lipids and a compensatory increase in fecal sterols occurred when cholesterol was added to the diet. Changes in serum lipoproteins in the subjects fed a \"prudent\" or a common diet indicate an increase in the proportion of HDL-cholesterol which may be beneficial in reducing coronary heart disease. Evidence suggests that, in absence of caloric excess, lowering of serum lipids and increased removal of fecal sterols is possible on a prudent diet without major modification of the customary foods, but that a high P:S ratio is required.", "contents": "Effect of unsaturated fats and cholesterol on serum and fecal lipids. A study of healthy middle-aged men. Modification of the Western diet, with an intake of 2,500 kcal per day and a decrease in cholesterol and fat intake to 33 per cent of calories with a P:S of 0.40, failed to alter serum lipids significantly. Evidence suggested that increasing the daily intake of cholesterol resulted in deposition in the body tissues. However, when the fatty acid composition and the P:S ratio of dietary fat were adjusted, lower serum lipids and a compensatory increase in fecal sterols occurred when cholesterol was added to the diet. Changes in serum lipoproteins in the subjects fed a \"prudent\" or a common diet indicate an increase in the proportion of HDL-cholesterol which may be beneficial in reducing coronary heart disease. Evidence suggests that, in absence of caloric excess, lowering of serum lipids and increased removal of fecal sterols is possible on a prudent diet without major modification of the customary foods, but that a high P:S ratio is required."}
{"id": "PMID:479484", "title": "Food habit changes of Chinese persons living in Lincoln, Nebraska.", "content": "Qualitative and quantitative changes in food habits of Chinese persons living in Lincoln, Nebraska, were evaluated. Findings indicated that these people do have a flexible attitude toward foods. Incorporation of American foods into their diet and a decreased consumption of Chinese foods showed that a continuous process of food-habit changing is occurring in this group. In particular, more American foods have been incorporated in their breakfast and lunch patterns, while for the dinner meal, the more traditional Chinese foods continue to be eaten. Factors related to the ease of adaptation to American food were investigated, and possible implications are suggested.", "contents": "Food habit changes of Chinese persons living in Lincoln, Nebraska. Qualitative and quantitative changes in food habits of Chinese persons living in Lincoln, Nebraska, were evaluated. Findings indicated that these people do have a flexible attitude toward foods. Incorporation of American foods into their diet and a decreased consumption of Chinese foods showed that a continuous process of food-habit changing is occurring in this group. In particular, more American foods have been incorporated in their breakfast and lunch patterns, while for the dinner meal, the more traditional Chinese foods continue to be eaten. Factors related to the ease of adaptation to American food were investigated, and possible implications are suggested."}
{"id": "PMID:479485", "title": "Ascorbic acid status of children with developmental disabilities.", "content": "Ascorbic acid status of thirty-nine white children with developmental disabilities, ages three to nineteen years, is reported. Mean daily ascorbic acid intakes were calculated from three-day food records. Biochemical assessment consisted of fasting serum levels and a 6-hr. load test. Nine children served as a control group for the load test only. Mean dietary intakes for the vitamin were 204 per cent of the allowance. The mean serum ascorbic acid value was 1.3 mg. per deciliter. Only two children had levels at the unacceptable deficient level. Following load tests, ten children were identified as low excretors (less than 17 per cent), nine were moderate excretors (17 to 23 per cent), and the rest were high excretors (above 23 per cent). All of the normal children were high excretors. Two of three children with low ascorbic acid intakes (below 66 per cent of the recommended allowance) were verified as deficient by their fasting serum levels and urinary recovery after a load.", "contents": "Ascorbic acid status of children with developmental disabilities. Ascorbic acid status of thirty-nine white children with developmental disabilities, ages three to nineteen years, is reported. Mean daily ascorbic acid intakes were calculated from three-day food records. Biochemical assessment consisted of fasting serum levels and a 6-hr. load test. Nine children served as a control group for the load test only. Mean dietary intakes for the vitamin were 204 per cent of the allowance. The mean serum ascorbic acid value was 1.3 mg. per deciliter. Only two children had levels at the unacceptable deficient level. Following load tests, ten children were identified as low excretors (less than 17 per cent), nine were moderate excretors (17 to 23 per cent), and the rest were high excretors (above 23 per cent). All of the normal children were high excretors. Two of three children with low ascorbic acid intakes (below 66 per cent of the recommended allowance) were verified as deficient by their fasting serum levels and urinary recovery after a load."}
{"id": "PMID:479486", "title": "Continuing education needs as perceived by dietetic practitioners.", "content": "Educational needs of dietitians were identified by 232 practitioners in Ohio by means of a self-administered questionnaire. Three major knowledge areas--managerial science, nutritional care science, and behavioral, communicative, and socio-cultural science--were covered in 109 questions. The respondents perceived a need for more professional knowledge than for behavioral, communicative, and socio-cultural sciences. Greater need was expressed for skills in technical and human ability than for skills in conceptual ability, and generally for nutritional knowledge rather than application. The findings could form the basis for continuing education programs.", "contents": "Continuing education needs as perceived by dietetic practitioners. Educational needs of dietitians were identified by 232 practitioners in Ohio by means of a self-administered questionnaire. Three major knowledge areas--managerial science, nutritional care science, and behavioral, communicative, and socio-cultural science--were covered in 109 questions. The respondents perceived a need for more professional knowledge than for behavioral, communicative, and socio-cultural sciences. Greater need was expressed for skills in technical and human ability than for skills in conceptual ability, and generally for nutritional knowledge rather than application. The findings could form the basis for continuing education programs."}
{"id": "PMID:479487", "title": "Work-related values and satisfactions. A cross-occupational analysis of professionals vs. non-professionals in hospital dietetic services.", "content": "Job satisfaction and work values of hospital dietitians were studied and compared with those of non-professional hospital foodservice personnel. The instrument was distributed to dietitians in nine Midwestern states. Dietitians had higher job satisfaction than foodservice employees on four components: The work itself, supervision, earnings, and compatibility with co-workers. Satisfaction increased with years in the profession, with directors of dietetics being more satisfied than staff dietitians. On the work value measures, dietitians scored higher on drive, ambition, and self-concept, whereas non-professional employees tended to attribute success to luck and \"knowing the right people.\"", "contents": "Work-related values and satisfactions. A cross-occupational analysis of professionals vs. non-professionals in hospital dietetic services. Job satisfaction and work values of hospital dietitians were studied and compared with those of non-professional hospital foodservice personnel. The instrument was distributed to dietitians in nine Midwestern states. Dietitians had higher job satisfaction than foodservice employees on four components: The work itself, supervision, earnings, and compatibility with co-workers. Satisfaction increased with years in the profession, with directors of dietetics being more satisfied than staff dietitians. On the work value measures, dietitians scored higher on drive, ambition, and self-concept, whereas non-professional employees tended to attribute success to luck and \"knowing the right people.\""}
{"id": "PMID:479489", "title": "Diet therapy in gastrointestinal disease: a commentary.", "content": "Dietary regimens in gastrointestinal disease can be divided into two categories: First, those of proven value include: (a) Disaccharide elimination for disaccharidase deficiency and exclusion of monosaccharides for sugar malabsorption; (b) gluten-free diet for celiac/sprue; (c) elimination of certain allergens because of food allergies; (d) protein restriction for portal systemic encephalopathy; (e) low-carbohydrate diet for dumping syndrome; (f) low-fiber diet for diarrheal syndromes; and (g) low-fat diet for steatorrhea. Second, controversial diets include a bland diet for acid-peptic disorders, a high-fiber diet for colonic disorders, and a low-fat diet for gallbladder disease. It is important to separate facts from fancy in the dietary management of patients with gastrointestinal disease and base the recommendations for a particular diet on available objective evidence, not on traditional or fashionable trends.", "contents": "Diet therapy in gastrointestinal disease: a commentary. Dietary regimens in gastrointestinal disease can be divided into two categories: First, those of proven value include: (a) Disaccharide elimination for disaccharidase deficiency and exclusion of monosaccharides for sugar malabsorption; (b) gluten-free diet for celiac/sprue; (c) elimination of certain allergens because of food allergies; (d) protein restriction for portal systemic encephalopathy; (e) low-carbohydrate diet for dumping syndrome; (f) low-fiber diet for diarrheal syndromes; and (g) low-fat diet for steatorrhea. Second, controversial diets include a bland diet for acid-peptic disorders, a high-fiber diet for colonic disorders, and a low-fat diet for gallbladder disease. It is important to separate facts from fancy in the dietary management of patients with gastrointestinal disease and base the recommendations for a particular diet on available objective evidence, not on traditional or fashionable trends."}
{"id": "PMID:479494", "title": "Diagnosis of pupillary anomalies.", "content": "Lesions of the afferent, intercalated, efferent (parasympathetic), and cervical sympathetic arms of the pupillary pathway each tend to produce rather distinctive clinical signs. Sophisticated equipment generally is not needed to define the probable basis for anisocoria and/or disordered pupillary function. Diagnosis of the more common anomalies of the pupil usually can be made from observations obtained using only a penlight, occluder, slit lamp biomicroscope, and a few diagnostic pharmaceutical agents. This paper has been written as a guide to recognition of pupillary signs and as an aid to the practitioner who must differentiate among those that mandate immediate referral, those which require careful analysis on a less urgent basis, and those that can be regarded, for all practical purposes, as benign.", "contents": "Diagnosis of pupillary anomalies. Lesions of the afferent, intercalated, efferent (parasympathetic), and cervical sympathetic arms of the pupillary pathway each tend to produce rather distinctive clinical signs. Sophisticated equipment generally is not needed to define the probable basis for anisocoria and/or disordered pupillary function. Diagnosis of the more common anomalies of the pupil usually can be made from observations obtained using only a penlight, occluder, slit lamp biomicroscope, and a few diagnostic pharmaceutical agents. This paper has been written as a guide to recognition of pupillary signs and as an aid to the practitioner who must differentiate among those that mandate immediate referral, those which require careful analysis on a less urgent basis, and those that can be regarded, for all practical purposes, as benign."}
{"id": "PMID:479495", "title": "Contrast sensitivity functions for clinical optometry.", "content": "Contrast sensitivity functions (CSF) provide a better measure of visual function than traditional acuity tests. The use of CSFs with cataract and contact lens patients as well as with patients suspected to have glaucoma or retrobulbar neuritis is discussed. Various methods for determining the CSF are discussed, with the conclusion that the newly developed Arden plates are best for use in clinical practice.", "contents": "Contrast sensitivity functions for clinical optometry. Contrast sensitivity functions (CSF) provide a better measure of visual function than traditional acuity tests. The use of CSFs with cataract and contact lens patients as well as with patients suspected to have glaucoma or retrobulbar neuritis is discussed. Various methods for determining the CSF are discussed, with the conclusion that the newly developed Arden plates are best for use in clinical practice."}
{"id": "PMID:479496", "title": "The amblyopic eye: is it downtrodden or just lazy?", "content": "Over the past decade or two considerable information has been compiled about the functioning of individual cells in mammalian visual cortex. Out of this data is developing a clearer understanding of the etiology of amblyopia. And with this clearer understanding is coming the development of new ideas for clinical practice in the prevention, treatment, and cure of amblyopia. While the leap from single-cell neurophysiology lab to optometric practice office seems like a huge one, the gap is narrowing; and taking the leap promises to provide a rich pay-off for vision health care.", "contents": "The amblyopic eye: is it downtrodden or just lazy? Over the past decade or two considerable information has been compiled about the functioning of individual cells in mammalian visual cortex. Out of this data is developing a clearer understanding of the etiology of amblyopia. And with this clearer understanding is coming the development of new ideas for clinical practice in the prevention, treatment, and cure of amblyopia. While the leap from single-cell neurophysiology lab to optometric practice office seems like a huge one, the gap is narrowing; and taking the leap promises to provide a rich pay-off for vision health care."}
{"id": "PMID:479497", "title": "The distance of rock test -- a preliminary report.", "content": "This paper is a preliminary report describing the development of a new clinical test for measuring the response time of the visual system to shift from distance to near and back to distance to a given discriminatory task. The new test is called Distance rock (Drk) and is calibrated in cycles per minute. Data from college students and survey results from 591 school children from the first to sixth grades were used to develop these preliminary results. Change in response time covariant with grade level was demonstrated which indicates this test may become clinically important for developmental vision.", "contents": "The distance of rock test -- a preliminary report. This paper is a preliminary report describing the development of a new clinical test for measuring the response time of the visual system to shift from distance to near and back to distance to a given discriminatory task. The new test is called Distance rock (Drk) and is calibrated in cycles per minute. Data from college students and survey results from 591 school children from the first to sixth grades were used to develop these preliminary results. Change in response time covariant with grade level was demonstrated which indicates this test may become clinically important for developmental vision."}
{"id": "PMID:479498", "title": "Effects of training on near-far response time as measured by the distance rock test.", "content": "The practice effects of specific visual training were demonstrated on twelve seventh grade students and seven optometry students using a distance rock test to measure distance-near response time. Fourteen practice sessions resulted in a 49% improvement for seventh grade students (range 15 to 78%) and a 40% improvement in cycles per minute for college students (range 24 to 64%). Errors were corresponding reduced as cycles per minute were increased.", "contents": "Effects of training on near-far response time as measured by the distance rock test. The practice effects of specific visual training were demonstrated on twelve seventh grade students and seven optometry students using a distance rock test to measure distance-near response time. Fourteen practice sessions resulted in a 49% improvement for seventh grade students (range 15 to 78%) and a 40% improvement in cycles per minute for college students (range 24 to 64%). Errors were corresponding reduced as cycles per minute were increased."}
{"id": "PMID:479516", "title": "Some reflections on boredom.", "content": "Boredom is a complex mental phenomenon, incorporating both affective and cognitive components, which cannot be understood within the confines of conflict theory alone. The paucity of fantasy in the bored person may reflect a developmental ego defect as well as, or instead of, the commonly-cited impulse-defense conflicts. The need for externally-supplied stimulation characteristic of persons with a field-dependent cognitive style, and/or characterological passivity may be among the significant predisposing factors for the development of boredom, and phase-specific factors may encourage its occurrence at particular periods of life. Boredom in the analyst, too, is multiply determined; the analytic situation itself contains features that contribute to it.", "contents": "Some reflections on boredom. Boredom is a complex mental phenomenon, incorporating both affective and cognitive components, which cannot be understood within the confines of conflict theory alone. The paucity of fantasy in the bored person may reflect a developmental ego defect as well as, or instead of, the commonly-cited impulse-defense conflicts. The need for externally-supplied stimulation characteristic of persons with a field-dependent cognitive style, and/or characterological passivity may be among the significant predisposing factors for the development of boredom, and phase-specific factors may encourage its occurrence at particular periods of life. Boredom in the analyst, too, is multiply determined; the analytic situation itself contains features that contribute to it."}
{"id": "PMID:479517", "title": "Methodological problems in the psychoanalytic interpretation of literature: a review of studies on Sophocles' Antigone.", "content": "Through a critical review of several studies dealing with Sophocles' drama, the Antigone, I have explored some of the prominent methodological problems encountered in the psychoanalytic interpretation of literature. Foremost among these is the inherent difficulty that the interpretation of literature is unable to benefit from the process of the analytic situation. Divorced from the realities of the therapeutic process, the drama itself is often used to corroborate an author's theoretical bias or to advance some special interest, with consequent distortion or blurring of the text. Although data about the artist's life and sociocultural environment may be of crucial significance, it is the text itself that must be the ultimate object of study. Through a re-examination of the Antigone as an aesthetic totality I have sketched out what appears to be an alternative manner of approaching the drama, and suggested that works of art reach us on both unconscious and conscious levels. I have stressed the need to analyze our emotional response to a work as affording a valuable source of insight into the work itself. Throughout, I have drawn attention to the need for greater scholarly rigor and the value of interdisciplinary collaboration. An open recognition of the problems in the psychoanalytic study of literature should serve to minimize dilettantism and raise the level of scholarship.", "contents": "Methodological problems in the psychoanalytic interpretation of literature: a review of studies on Sophocles' Antigone. Through a critical review of several studies dealing with Sophocles' drama, the Antigone, I have explored some of the prominent methodological problems encountered in the psychoanalytic interpretation of literature. Foremost among these is the inherent difficulty that the interpretation of literature is unable to benefit from the process of the analytic situation. Divorced from the realities of the therapeutic process, the drama itself is often used to corroborate an author's theoretical bias or to advance some special interest, with consequent distortion or blurring of the text. Although data about the artist's life and sociocultural environment may be of crucial significance, it is the text itself that must be the ultimate object of study. Through a re-examination of the Antigone as an aesthetic totality I have sketched out what appears to be an alternative manner of approaching the drama, and suggested that works of art reach us on both unconscious and conscious levels. I have stressed the need to analyze our emotional response to a work as affording a valuable source of insight into the work itself. Throughout, I have drawn attention to the need for greater scholarly rigor and the value of interdisciplinary collaboration. An open recognition of the problems in the psychoanalytic study of literature should serve to minimize dilettantism and raise the level of scholarship."}
{"id": "PMID:479518", "title": "[Comparison of the action of an impeded estrogen, frideron, and of estradiol on the seminal vesicles and on the weight of the testicles and accessory sex glands in the rat. II].", "content": "We have undertaken a comparative study on the effects of estradiol and of an impeded estrogen, zeranol, in the prepubescent Wistar male rat. Both had an inhibitory effect on the secretion of F.S.H. in the spermatogenesis test. Nine injections of estradiol or nine ingestions of zeranol had a restraining effect with a dose of 0.5 microgram of estradiol or of 500 micrograms of zeranol (for 100 g of body weight). With some animals, spermatogenesis is interrupted and the weight of the testicles decreased; the treatment is more active with stronger doses: 2 micrograms of estradiol interrupted spermatogenesis in 10 rats out of 11, 1.000 microgram of zeranol interrupted it in 10 rats out of 13. A weak dose of either estrogen (0.05 microgram of estradiol or 20 micrograms of zeranol) stimulated the growth of the seminal vesicles, the prostate gland and the preputial glands. Strong doses decrease the weight of the preputial glands, and even more the weight of the prostate gland (10 micrograms of estradiol, 1.000 microgram of zeranol). They are ineffective on the seminal vesicles. The effects of 2 micrograms of estradiol on the testicles and the accessory sexual glands are completely inhibited by the simultaneous administration of 2 U.I. of P.M.S. rich in FSH.", "contents": "[Comparison of the action of an impeded estrogen, frideron, and of estradiol on the seminal vesicles and on the weight of the testicles and accessory sex glands in the rat. II]. We have undertaken a comparative study on the effects of estradiol and of an impeded estrogen, zeranol, in the prepubescent Wistar male rat. Both had an inhibitory effect on the secretion of F.S.H. in the spermatogenesis test. Nine injections of estradiol or nine ingestions of zeranol had a restraining effect with a dose of 0.5 microgram of estradiol or of 500 micrograms of zeranol (for 100 g of body weight). With some animals, spermatogenesis is interrupted and the weight of the testicles decreased; the treatment is more active with stronger doses: 2 micrograms of estradiol interrupted spermatogenesis in 10 rats out of 11, 1.000 microgram of zeranol interrupted it in 10 rats out of 13. A weak dose of either estrogen (0.05 microgram of estradiol or 20 micrograms of zeranol) stimulated the growth of the seminal vesicles, the prostate gland and the preputial glands. Strong doses decrease the weight of the preputial glands, and even more the weight of the prostate gland (10 micrograms of estradiol, 1.000 microgram of zeranol). They are ineffective on the seminal vesicles. The effects of 2 micrograms of estradiol on the testicles and the accessory sexual glands are completely inhibited by the simultaneous administration of 2 U.I. of P.M.S. rich in FSH."}
{"id": "PMID:479519", "title": "[The prognostic value of raised uric acid in the blood in arterial hypertension in pregnancy. 58 cases (author's transl)].", "content": "The authors have carried out research in 58 cases to see whether the findings of high uric acid levels in the blood are of prognostic value in all cases of arterial hypertension in pregnancy whatever the aetiology. The material studied consisted of 30 true cases of toxaemia of pregnancy, 13 cases of recurrent toxaemia and 15 cases of the vasculo-renal syndrome on top of the pre-existing pathological condition. In 37 cases where the blood uric acid level was lower than 300 micromoles there were only 4 minor complications. Serious complications were found in 6 cases in which the patients had a blood uric acid level highthan 480 micromoles. This study confirms the prognostic value of blood uric acid levels. The estimation of these levels, however, seems to be of less value in making a differential diagnosis between the various kinds of toxaemia and hypertension found in pregnant women due to different aetiologies.", "contents": "[The prognostic value of raised uric acid in the blood in arterial hypertension in pregnancy. 58 cases (author's transl)]. The authors have carried out research in 58 cases to see whether the findings of high uric acid levels in the blood are of prognostic value in all cases of arterial hypertension in pregnancy whatever the aetiology. The material studied consisted of 30 true cases of toxaemia of pregnancy, 13 cases of recurrent toxaemia and 15 cases of the vasculo-renal syndrome on top of the pre-existing pathological condition. In 37 cases where the blood uric acid level was lower than 300 micromoles there were only 4 minor complications. Serious complications were found in 6 cases in which the patients had a blood uric acid level highthan 480 micromoles. This study confirms the prognostic value of blood uric acid levels. The estimation of these levels, however, seems to be of less value in making a differential diagnosis between the various kinds of toxaemia and hypertension found in pregnant women due to different aetiologies."}
{"id": "PMID:479520", "title": "[The correlation between sonar morphological measurements and plasma levels of oestradiol and oestriol in early pregnancy (author's transl)].", "content": "53 cases of pregnancy complicated by bleeding between the 7th and 16th week have been studied by repeated estimations of non-conjugated plasma oestradiol and oestriol carried out at the same time as ultrasound morphological measurements were performed. 24 pregnancies went on to term while 29 met with the loss of the fetus. In each case where the outcome was favourable the plasma oestriol and oestradiol levels were comparable to those found in 95 control pregnant women whose pregnancies progressed normally. In 26 cases where the outcome was unfavourable the levels were too low and corresponded to an ultrasound picture that was abnormal or revealed absence of heart beat. In 3 cases the first levels of oestradiol and oestriol seemed to be normal. Later, when failure of growth of the embryo and absence of heart beat were found, a considerable drop in the levels of oestradiol and of oestriol in the blood was also revealed. This study shows a good correlation between hormone levels and ultrasound findings in pregnancies complicated by bleeding. All the same, the prognostic value of hormone measurements as compared with ultrasound measurements has not yet been established, and needs analysis of a far greater number of cases of a similar type.", "contents": "[The correlation between sonar morphological measurements and plasma levels of oestradiol and oestriol in early pregnancy (author's transl)]. 53 cases of pregnancy complicated by bleeding between the 7th and 16th week have been studied by repeated estimations of non-conjugated plasma oestradiol and oestriol carried out at the same time as ultrasound morphological measurements were performed. 24 pregnancies went on to term while 29 met with the loss of the fetus. In each case where the outcome was favourable the plasma oestriol and oestradiol levels were comparable to those found in 95 control pregnant women whose pregnancies progressed normally. In 26 cases where the outcome was unfavourable the levels were too low and corresponded to an ultrasound picture that was abnormal or revealed absence of heart beat. In 3 cases the first levels of oestradiol and oestriol seemed to be normal. Later, when failure of growth of the embryo and absence of heart beat were found, a considerable drop in the levels of oestradiol and of oestriol in the blood was also revealed. This study shows a good correlation between hormone levels and ultrasound findings in pregnancies complicated by bleeding. All the same, the prognostic value of hormone measurements as compared with ultrasound measurements has not yet been established, and needs analysis of a far greater number of cases of a similar type."}
{"id": "PMID:479521", "title": "[A contribution to the study of ovarian cancer during pregnancy and the puerperium. 3 case histories (author's transl)].", "content": "The authors have seen three cancers out of 27 organic ovarian tumours which were diagnosed and operated on during pregnancy and the puerperium in 16790 deliveries. They recall the circumstances under which the condition was discovered and the difficulties in diagnosing it. They point out particularly the value of ultrasound and frozen section histological examination. They point out the therapeutic problems which are often of a dramatic nature because of the association of the two conditions and the prognosis which in turn depends mainly on the stage of the lesion and the operability of the tumour. Poor maternal prognosis in cases of ovarian cancer is an indication for operation before the 20th week of pregnancy of all tumours of the ovary which have been clinically diagnosed and confirmed by ultrasound.", "contents": "[A contribution to the study of ovarian cancer during pregnancy and the puerperium. 3 case histories (author's transl)]. The authors have seen three cancers out of 27 organic ovarian tumours which were diagnosed and operated on during pregnancy and the puerperium in 16790 deliveries. They recall the circumstances under which the condition was discovered and the difficulties in diagnosing it. They point out particularly the value of ultrasound and frozen section histological examination. They point out the therapeutic problems which are often of a dramatic nature because of the association of the two conditions and the prognosis which in turn depends mainly on the stage of the lesion and the operability of the tumour. Poor maternal prognosis in cases of ovarian cancer is an indication for operation before the 20th week of pregnancy of all tumours of the ovary which have been clinically diagnosed and confirmed by ultrasound."}
{"id": "PMID:479522", "title": "[Celioscopic sterilizations with Yoon rings. 300 cases. Immediate complications, effectiveness and reversibility].", "content": "The authors report on a series of 370 sterilisations using Yoon's rings, of which 300 were carried out laparoscopically. They confirm the efficiency of the method and its safety because there was only minimal operative morbidity. On the other hand this technique offers the possibilities of reversal of sterilisation in such a way that it should be the preferred method to be used systematically when a young woman is being sterilised.", "contents": "[Celioscopic sterilizations with Yoon rings. 300 cases. Immediate complications, effectiveness and reversibility]. The authors report on a series of 370 sterilisations using Yoon's rings, of which 300 were carried out laparoscopically. They confirm the efficiency of the method and its safety because there was only minimal operative morbidity. On the other hand this technique offers the possibilities of reversal of sterilisation in such a way that it should be the preferred method to be used systematically when a young woman is being sterilised."}
{"id": "PMID:479540", "title": "Histopathology of experimental Schistosoma incognitum infection in mice following exposure to normal and irradiated cercariae.", "content": "The tissue reactions in mice, experimentally infected with normal and irradiated cercariae of S. incognitum were studied. The lesions observed in the skin, liver, lungs and the intestine of mice infected with normal cercariae are briefly described, and compared with those observed with cercariae irradiated at 3000 r of gamma rays. In general, the reactions in mice exposed to normal cercariae were more intense than in those infected with irradiated cercariae. The severity of the reactions appeared largely due to the deposition of eggs in the tissues of the mice infected with normal cercariae. The experimental evidence suggested that most of the flukes from the irradiated cercariae are destroyed in the liver by tissue reaction.", "contents": "Histopathology of experimental Schistosoma incognitum infection in mice following exposure to normal and irradiated cercariae. The tissue reactions in mice, experimentally infected with normal and irradiated cercariae of S. incognitum were studied. The lesions observed in the skin, liver, lungs and the intestine of mice infected with normal cercariae are briefly described, and compared with those observed with cercariae irradiated at 3000 r of gamma rays. In general, the reactions in mice exposed to normal cercariae were more intense than in those infected with irradiated cercariae. The severity of the reactions appeared largely due to the deposition of eggs in the tissues of the mice infected with normal cercariae. The experimental evidence suggested that most of the flukes from the irradiated cercariae are destroyed in the liver by tissue reaction."}
{"id": "PMID:479541", "title": "Host-parasite relationship of Angiostrongylus cantonensis. 1. Intracranial transplantation into various hosts.", "content": "Various stages of Angiostrongylus cantonensis recovered from the brain of experimentally infected mice were transplanted intracranially into rats. Third and fourth-stage worms recovered 2--7 days postinfection were able to develop normally after transplantation into recipient rats. The fifth-stage worm obtained 14--15 days postinfection would enter the brain tissue of rats but died shortly afterwards. However, the same stage of worms recovered from rats, after a similar transplantation, were found to develop normally in the recipient animal. Young fifth-stage worms, from the subarachnoid space of rats, which were ready for the pulmonary migration were also transplanted into rabbits but the worms failed to reach the lungs. In the control rat-to-rat transplantation, the worms successfully completed the pulmonary migration. The morphogenesis and initial growth rate of A. cantonensis were similar in both mice and rats but in the former host the worms started to grow at a markedly slower rate after the last moult and gradually degenerated.", "contents": "Host-parasite relationship of Angiostrongylus cantonensis. 1. Intracranial transplantation into various hosts. Various stages of Angiostrongylus cantonensis recovered from the brain of experimentally infected mice were transplanted intracranially into rats. Third and fourth-stage worms recovered 2--7 days postinfection were able to develop normally after transplantation into recipient rats. The fifth-stage worm obtained 14--15 days postinfection would enter the brain tissue of rats but died shortly afterwards. However, the same stage of worms recovered from rats, after a similar transplantation, were found to develop normally in the recipient animal. Young fifth-stage worms, from the subarachnoid space of rats, which were ready for the pulmonary migration were also transplanted into rabbits but the worms failed to reach the lungs. In the control rat-to-rat transplantation, the worms successfully completed the pulmonary migration. The morphogenesis and initial growth rate of A. cantonensis were similar in both mice and rats but in the former host the worms started to grow at a markedly slower rate after the last moult and gradually degenerated."}
{"id": "PMID:479544", "title": "A new technique for the recovery of Toxocara eggs from soil.", "content": "A new flotation technique for the extraction of Toxocara eggs from soil has been devised. It has a fairly high recovery efficiency for Toxocara eggs, can be used for replicated quantitative experiments, and may also be used for recovery of other ascarid eggs from soil.", "contents": "A new technique for the recovery of Toxocara eggs from soil. A new flotation technique for the extraction of Toxocara eggs from soil has been devised. It has a fairly high recovery efficiency for Toxocara eggs, can be used for replicated quantitative experiments, and may also be used for recovery of other ascarid eggs from soil."}
{"id": "PMID:479545", "title": "Prevalence of Toxocara spp. eggs in some public grounds and highway rest areas in Kansas.", "content": "Out of a total of 282 soil samples obtained from public areas, 58 samples contained Toxocara spp. eggs. This gave an overall prevalence of 20.6%. Highway rest areas were contaminated and childrens sand boxes had the highest percentage of recovery of all.", "contents": "Prevalence of Toxocara spp. eggs in some public grounds and highway rest areas in Kansas. Out of a total of 282 soil samples obtained from public areas, 58 samples contained Toxocara spp. eggs. This gave an overall prevalence of 20.6%. Highway rest areas were contaminated and childrens sand boxes had the highest percentage of recovery of all."}
{"id": "PMID:479546", "title": "Preliminary survey of Capillaria hepatica (Bancroft, 1893) in Malaysia.", "content": "The prevalence of Capillaria hepatica (Bancroft, 1893) infection in a total of 2324 rats trapped from 25 localities in West Malaysia was 15.5%. Infection rates in males (16.0%) and females (15.1%) are similar. A significantly higher percentage of adults (18.1%) than young (7.7%) was infected. Capillaria hepatica infection rates among urban (0.7%) and jungle (0.0%) rats was very low as compared to field rats (17.7%) trapped from agricultural areas such as oil palm estates and rice growing areas. Prevalence of C. hepatica infection in rats is not evenly distributed throughout West Malaysia. There seem to be localised foci of infection. In some areas as many as 77.8% of the adult rats are found to be infected while in other areas the same species of rats are found free of infection.", "contents": "Preliminary survey of Capillaria hepatica (Bancroft, 1893) in Malaysia. The prevalence of Capillaria hepatica (Bancroft, 1893) infection in a total of 2324 rats trapped from 25 localities in West Malaysia was 15.5%. Infection rates in males (16.0%) and females (15.1%) are similar. A significantly higher percentage of adults (18.1%) than young (7.7%) was infected. Capillaria hepatica infection rates among urban (0.7%) and jungle (0.0%) rats was very low as compared to field rats (17.7%) trapped from agricultural areas such as oil palm estates and rice growing areas. Prevalence of C. hepatica infection in rats is not evenly distributed throughout West Malaysia. There seem to be localised foci of infection. In some areas as many as 77.8% of the adult rats are found to be infected while in other areas the same species of rats are found free of infection."}
{"id": "PMID:479547", "title": "Effect of diethylcarbamazine on the concentration of Onchocerca volvulus microfilariae in hydrocoele fluid and urine.", "content": "The effect of graded doses of diethylcarbamazine on the concentration of microfilariae in the hydrocoele fluid, urine and skin of patients suffering from onchocerciasis was studied. The results showed that a significant number of microfilariae migrated into hydrocoele fluid and urine immediately after the drug was given and thereafter returned to pre-treatment levels rather more quickly in the former than the latter. The increase was found to be roughly directly proportional to the intensity of infection in the skin. By contrast the skin concentration of microfilariae fell sharply after treatment and remained significantly lower than pre-treatment levels for over four weeks. The potential use to which these observations could be put with respect to clinical screening of drugs in onchocerciasis is discussed.", "contents": "Effect of diethylcarbamazine on the concentration of Onchocerca volvulus microfilariae in hydrocoele fluid and urine. The effect of graded doses of diethylcarbamazine on the concentration of microfilariae in the hydrocoele fluid, urine and skin of patients suffering from onchocerciasis was studied. The results showed that a significant number of microfilariae migrated into hydrocoele fluid and urine immediately after the drug was given and thereafter returned to pre-treatment levels rather more quickly in the former than the latter. The increase was found to be roughly directly proportional to the intensity of infection in the skin. By contrast the skin concentration of microfilariae fell sharply after treatment and remained significantly lower than pre-treatment levels for over four weeks. The potential use to which these observations could be put with respect to clinical screening of drugs in onchocerciasis is discussed."}
{"id": "PMID:479552", "title": "The occurrence and distribution of H-2 antigens on mouse intestinal epithelial cells.", "content": "This report describes an immunoferritin labeling study of mouse H-2 histocompatibility antigens on epithelial cells dissociated from the small intestine by EDTA and trypsin. Before cell dissociation, the intestine was prefixed in paraformaldehyde or periodate-lysine-paraformaldehyde in order to preserve the shape of the cells and to immobilize H-2 antigens in their native positions. The results demonstrated the presence of H-2 antigens on the lateral and basal cell membranes at about the same high density that was observed at the surface of mouse monocytes. No H-2 antigens could be detected at the apical surface of dissociated or undissociated epithelial cells. It is unlikely that the fuzzy coat masked H-2 antigens at the apical surface because it was essentially absent from the apical membranes of dissociated cells. These observations extend our knowledge of the cellular distribution of transplantation antigens, and provide further evidence of a discontinuity in the expression of membrane components at the junctional complex of epithelial cells.", "contents": "The occurrence and distribution of H-2 antigens on mouse intestinal epithelial cells. This report describes an immunoferritin labeling study of mouse H-2 histocompatibility antigens on epithelial cells dissociated from the small intestine by EDTA and trypsin. Before cell dissociation, the intestine was prefixed in paraformaldehyde or periodate-lysine-paraformaldehyde in order to preserve the shape of the cells and to immobilize H-2 antigens in their native positions. The results demonstrated the presence of H-2 antigens on the lateral and basal cell membranes at about the same high density that was observed at the surface of mouse monocytes. No H-2 antigens could be detected at the apical surface of dissociated or undissociated epithelial cells. It is unlikely that the fuzzy coat masked H-2 antigens at the apical surface because it was essentially absent from the apical membranes of dissociated cells. These observations extend our knowledge of the cellular distribution of transplantation antigens, and provide further evidence of a discontinuity in the expression of membrane components at the junctional complex of epithelial cells."}
{"id": "PMID:479553", "title": "Cytochemical demonstration of adenylate cyclase in cardiac muscle: effect of dimethyl sulfoxide.", "content": "Adenylate cyclase (AC) activity was evaluated after perfusion fixation of rat and dog myocardium with 4% paraformaldehyde (PFA), 2% glutaraldehyde (GA) or a combination of both, in cacodylate buffer. Dimethyl sulfoxide (DMSO) was added to the fixatives and its effect on the preservation of cell organelles and enzyme activity was determined. Adenylate cyclase activity was preserved best after fixation with 4% paraformaldehyde but this fixative did not provide for optimal maintenance of structure. Prefixation with 2% glutaraldehyde and 5% dimethyl sulfoxide provided the most effective preservation of both structural and enzymatic integrity. Precipitation of lead diphosphoimide was the morphologic indicator of sites of adenylate cyclase activity. The most intense precipitate was in the lumen of junctional sarcoplasmic reticulum in close contact with T-tubules and in subsarcolemmal cisternae. Evidence of activity was also seen on the intracellular aspect of the sarcolemmal membrane and in the nexus segment of the intercalated discs. Alloxan was effective as an inhibitor of adenylate cyclase activity only if the concentration of the activating substance sodium fluoride (NaF) was 20 mM or lower.", "contents": "Cytochemical demonstration of adenylate cyclase in cardiac muscle: effect of dimethyl sulfoxide. Adenylate cyclase (AC) activity was evaluated after perfusion fixation of rat and dog myocardium with 4% paraformaldehyde (PFA), 2% glutaraldehyde (GA) or a combination of both, in cacodylate buffer. Dimethyl sulfoxide (DMSO) was added to the fixatives and its effect on the preservation of cell organelles and enzyme activity was determined. Adenylate cyclase activity was preserved best after fixation with 4% paraformaldehyde but this fixative did not provide for optimal maintenance of structure. Prefixation with 2% glutaraldehyde and 5% dimethyl sulfoxide provided the most effective preservation of both structural and enzymatic integrity. Precipitation of lead diphosphoimide was the morphologic indicator of sites of adenylate cyclase activity. The most intense precipitate was in the lumen of junctional sarcoplasmic reticulum in close contact with T-tubules and in subsarcolemmal cisternae. Evidence of activity was also seen on the intracellular aspect of the sarcolemmal membrane and in the nexus segment of the intercalated discs. Alloxan was effective as an inhibitor of adenylate cyclase activity only if the concentration of the activating substance sodium fluoride (NaF) was 20 mM or lower."}
{"id": "PMID:479548", "title": "Effect of rabbit strain on activity level and cytotoxicity of serum complement. III. Comparison of four tumor target cells.", "content": "Serum samples from about 10 males and 10 females from each of 15 genetically defined strains of rabbits and from one hybrid were tested as sources of complement for the microtiter lymphocytotoxicity test using tumor cells from SaI-A, 6C3HED-A, BW5147, and L-cells as target cells. The tumor cells were tested with appropriate H-2 antisera. Results indicated strain differences particularly evident when the target cells were eithter 6C3HED-A or L-cells. BW5147 worked well with most all strains and SaI-A was extremely demanding of all strains of rabbits. However, strains IIIC/J, IIIVO/J and the F1 hybrid between them were still among the six best strains when tested against the tumor cells as they were when tested against the lymph nodes in previously published data. Strain WH/J which carries the gene, ha, for hereditary lymphosarcoma, proved to be a good complement source with SaI-A and 6C3HED-A, both sarcoma in nature. However, it was not a good complement source with BW5147, a lymphatic leukemia, or L-cells. This is possible evidence for a common tumor antigen associated with lymphosarcoma.", "contents": "Effect of rabbit strain on activity level and cytotoxicity of serum complement. III. Comparison of four tumor target cells. Serum samples from about 10 males and 10 females from each of 15 genetically defined strains of rabbits and from one hybrid were tested as sources of complement for the microtiter lymphocytotoxicity test using tumor cells from SaI-A, 6C3HED-A, BW5147, and L-cells as target cells. The tumor cells were tested with appropriate H-2 antisera. Results indicated strain differences particularly evident when the target cells were eithter 6C3HED-A or L-cells. BW5147 worked well with most all strains and SaI-A was extremely demanding of all strains of rabbits. However, strains IIIC/J, IIIVO/J and the F1 hybrid between them were still among the six best strains when tested against the tumor cells as they were when tested against the lymph nodes in previously published data. Strain WH/J which carries the gene, ha, for hereditary lymphosarcoma, proved to be a good complement source with SaI-A and 6C3HED-A, both sarcoma in nature. However, it was not a good complement source with BW5147, a lymphatic leukemia, or L-cells. This is possible evidence for a common tumor antigen associated with lymphosarcoma."}
{"id": "PMID:479555", "title": "On the immunocytochemical localization of the vasoactive intestinal polypeptide.", "content": "The distribution of vasoactive intestinal polypeptide (VIP) immunoreactive nerves and endocrine cells in the gastrointestinal tract and pancreas of a number of mammalian and submammalian species has been examined in order to throw light on the exact localization of this peptide. Seven out of 8 VIP antisera demonstrated numerous nerve fibers in the gut, whereas one antiserum (TR2) revealed only scattered, few nerve fibers. The distribution of endocrine cells demonstrated by the different VIP antisera varied considerably. Thus, some antisera demonstrated only endocrine cells in the feline antrum, others only colonic endocrine cells and still others only endocrine cells of the upper gut and pancreas. The variability in staining pattern of endocrine cells as well as recent radioimmunological data makes it opportune to suggest that true VIP is a neuronal peptide and that endocrine cells store peptides resembling, but not being identical with, VIP (VIPoids).", "contents": "On the immunocytochemical localization of the vasoactive intestinal polypeptide. The distribution of vasoactive intestinal polypeptide (VIP) immunoreactive nerves and endocrine cells in the gastrointestinal tract and pancreas of a number of mammalian and submammalian species has been examined in order to throw light on the exact localization of this peptide. Seven out of 8 VIP antisera demonstrated numerous nerve fibers in the gut, whereas one antiserum (TR2) revealed only scattered, few nerve fibers. The distribution of endocrine cells demonstrated by the different VIP antisera varied considerably. Thus, some antisera demonstrated only endocrine cells in the feline antrum, others only colonic endocrine cells and still others only endocrine cells of the upper gut and pancreas. The variability in staining pattern of endocrine cells as well as recent radioimmunological data makes it opportune to suggest that true VIP is a neuronal peptide and that endocrine cells store peptides resembling, but not being identical with, VIP (VIPoids)."}
{"id": "PMID:479549", "title": "Reproduction and teratogenic studies of 5-thio-D-glucose in mice.", "content": "Under the conditions of the present study, 5-thio-D-glucose was found to be an effective and useful reversible male contraceptive. Treatment of male mice with 5-thio, administered intraperitoneally or orally at 40 or 60 mg/kg for 28 to 35 days produced germinal cell degeneration and complete inhibition of spermatogenesis in the seminiferous tubules. These treatment regimens also resulted in complete sterility in males lasting for several weeks. Resumption of sperm development and fertility occurred with 5 to 6 weeks after treatment was discontinued. The Leydig cell population, Sertoli cells, and spermatogonia apparently were not affected and male libido was not impaired. There was no evidence of embryotoxic or teratogenic effect in litters of pregnant females treated orally with 5-thio on days 6--12 of gestation.", "contents": "Reproduction and teratogenic studies of 5-thio-D-glucose in mice. Under the conditions of the present study, 5-thio-D-glucose was found to be an effective and useful reversible male contraceptive. Treatment of male mice with 5-thio, administered intraperitoneally or orally at 40 or 60 mg/kg for 28 to 35 days produced germinal cell degeneration and complete inhibition of spermatogenesis in the seminiferous tubules. These treatment regimens also resulted in complete sterility in males lasting for several weeks. Resumption of sperm development and fertility occurred with 5 to 6 weeks after treatment was discontinued. The Leydig cell population, Sertoli cells, and spermatogonia apparently were not affected and male libido was not impaired. There was no evidence of embryotoxic or teratogenic effect in litters of pregnant females treated orally with 5-thio on days 6--12 of gestation."}
{"id": "PMID:479550", "title": "Repeated epilation: a genetic epidermal syndrome in mice.", "content": "Repeated epilation is an autosomal semidominant mutation located on chromosome 4 of the house mouse. Heterozygotes for Er show irregular hairloss. Homozygotes die immediately after birth from acute respiratory distress and exhibit an hypoplastic epidermal syndrome associated with morphological modifications of the oral cavity and of the limbs.", "contents": "Repeated epilation: a genetic epidermal syndrome in mice. Repeated epilation is an autosomal semidominant mutation located on chromosome 4 of the house mouse. Heterozygotes for Er show irregular hairloss. Homozygotes die immediately after birth from acute respiratory distress and exhibit an hypoplastic epidermal syndrome associated with morphological modifications of the oral cavity and of the limbs."}
{"id": "PMID:479556", "title": "Immunohistochemical characterization of monolayer cell cultures of embryonic chicken pancreas and measurement of somatostatin release.", "content": "Monolayer cell cultures of embryonic chicken pancreas contain functionally active insulin, glucagon and somatostatin-containing cells as evidenced by immunohistochemical and radioimmunoassay techniques. Hormone release is in relation to the number of each cell type present and responds to known specific secretory stimuli. The relatively high numbers of D-cells and amounts of immunoreactive somatostatin released by this preparation makes this system a suitable model for studies of somatostatin function and secretion.", "contents": "Immunohistochemical characterization of monolayer cell cultures of embryonic chicken pancreas and measurement of somatostatin release. Monolayer cell cultures of embryonic chicken pancreas contain functionally active insulin, glucagon and somatostatin-containing cells as evidenced by immunohistochemical and radioimmunoassay techniques. Hormone release is in relation to the number of each cell type present and responds to known specific secretory stimuli. The relatively high numbers of D-cells and amounts of immunoreactive somatostatin released by this preparation makes this system a suitable model for studies of somatostatin function and secretion."}
{"id": "PMID:479557", "title": "Immunocytochemical localization of hepatic legandin and Z protein utilizing frozen sections for light and electron microscopy.", "content": "Ligandin (glutathione-s-transferase) and Z protein are soluble hepatocellular proteins that are involved in the transfer of organic ions, including bilirubin and some hormones and carcinogens from the plasma to the liver. The intracellular distribution of ligandin and Z protein was studied by applying the peroxidase-antiperoxidase procedure of L. A. Sternberger (Immunocytochemistry, Prentice Hall Inc., 1974) to paraffin sections and free-floating 10-micrometers frozen sections that were processed for both light and electron microscopy. Ligandin and Z protein were localized to the cytosol of hepatocytes in association with smooth endoplasmic reticulum (SER), but no reaction product was present between cisternae of rough endoplasmic reticulum. Penetration of reagents was enhanced in 10-micrometers frozen sections and the preservation of subcellular structures was equivalent to thicker, unfrozen sections.", "contents": "Immunocytochemical localization of hepatic legandin and Z protein utilizing frozen sections for light and electron microscopy. Ligandin (glutathione-s-transferase) and Z protein are soluble hepatocellular proteins that are involved in the transfer of organic ions, including bilirubin and some hormones and carcinogens from the plasma to the liver. The intracellular distribution of ligandin and Z protein was studied by applying the peroxidase-antiperoxidase procedure of L. A. Sternberger (Immunocytochemistry, Prentice Hall Inc., 1974) to paraffin sections and free-floating 10-micrometers frozen sections that were processed for both light and electron microscopy. Ligandin and Z protein were localized to the cytosol of hepatocytes in association with smooth endoplasmic reticulum (SER), but no reaction product was present between cisternae of rough endoplasmic reticulum. Penetration of reagents was enhanced in 10-micrometers frozen sections and the preservation of subcellular structures was equivalent to thicker, unfrozen sections."}
{"id": "PMID:479558", "title": "Morphometrical analysis of an ultrahistochemical demonstration of nonspecific esterases in hepatocytes of mice after fructose overload.", "content": "Changes in ultrastructural distribution patterns of nonspecific esterases (E.C. 3.1.19) are described quantitatively by means of morphometry. Esterases were demonstrated with O-acetyl-8-hydroxyquinoline (Q-O-2) and S-acetyl-8-mercaptoquinoline in livers of normally and exclusively fructose-fed mice. Conditions are discussed, under which the quantification of the ultrastructural products of enzyme histochemical reactions may be possible. Smooth endoplasmic reticulum and rough endoplasmic reticulum exhibit no alteration in enzyme distribution with both substrates since the enzyme-occupied proportion of each compartment remains the same despite an overall decrease of both compartments. Likewise an increase of O-acetyl-8-hydroxyquinoline-esterase at fat droplets corresponds to the increase in total surface of the fat. S-acetyl-8-mercaptoquinoline-positive fat surface however reveals an increase far beyond that of the total fat surface. The results support the hypothesis that a variety of esterases with different substrate spectra are present at the subcellular level in different cell compartments.", "contents": "Morphometrical analysis of an ultrahistochemical demonstration of nonspecific esterases in hepatocytes of mice after fructose overload. Changes in ultrastructural distribution patterns of nonspecific esterases (E.C. 3.1.19) are described quantitatively by means of morphometry. Esterases were demonstrated with O-acetyl-8-hydroxyquinoline (Q-O-2) and S-acetyl-8-mercaptoquinoline in livers of normally and exclusively fructose-fed mice. Conditions are discussed, under which the quantification of the ultrastructural products of enzyme histochemical reactions may be possible. Smooth endoplasmic reticulum and rough endoplasmic reticulum exhibit no alteration in enzyme distribution with both substrates since the enzyme-occupied proportion of each compartment remains the same despite an overall decrease of both compartments. Likewise an increase of O-acetyl-8-hydroxyquinoline-esterase at fat droplets corresponds to the increase in total surface of the fat. S-acetyl-8-mercaptoquinoline-positive fat surface however reveals an increase far beyond that of the total fat surface. The results support the hypothesis that a variety of esterases with different substrate spectra are present at the subcellular level in different cell compartments."}
{"id": "PMID:479559", "title": "Tissue fixation by osmium tetroxide. A possible role for proteins.", "content": "The osmiophilia, under the conditions of normal tissue fixation, of the histidine, lysine, tryptophan, cysteine and methionine side chain of proteins is suggested by in vitro studies on blocked amino acids representative of such protein side chains, and the chemical nature of the reaction products elucidated. The chemical feasibility of inter- or intramolecular cross-linking of protein by OsO4 at these and other sites is demonstrated, as in the cross-linking of protein with unsaturated lipids such as methyl oleate, methyl linoleate and linolenate, and cholesteryl acetate. The relevance of these results to the process of tissue fixation by OsO4 is discussed.", "contents": "Tissue fixation by osmium tetroxide. A possible role for proteins. The osmiophilia, under the conditions of normal tissue fixation, of the histidine, lysine, tryptophan, cysteine and methionine side chain of proteins is suggested by in vitro studies on blocked amino acids representative of such protein side chains, and the chemical nature of the reaction products elucidated. The chemical feasibility of inter- or intramolecular cross-linking of protein by OsO4 at these and other sites is demonstrated, as in the cross-linking of protein with unsaturated lipids such as methyl oleate, methyl linoleate and linolenate, and cholesteryl acetate. The relevance of these results to the process of tissue fixation by OsO4 is discussed."}
{"id": "PMID:479560", "title": "The use of lectins and cholera toxin for the detection of surface carbohydrates of cultured neurons and neuroblastoma.", "content": "Conjugates of horseradish peroxidase with the lectins ricin (d-galactose), wheat germ agglutinin (N-acetylglucosamine), phytohemagglutinin (N-acetylgalactosamine), and with cholera toxin (GM1 ganglioside) were used for a cytochemical detection of corresponding termin al carbohydrates, or glycolipids on cell surfaces of cultured neurons and neuroblastoma cells. Cells were labeled at 4 degrees C with the above ligands and their adsorptive endocytosis was studied after incubations at 37 degrees C in a medium free of ligand. Peroxidase was detected by the method of Graham and Karnovsky (J. Histochem. Cytochem. 14:291, 1966). Lectins and cholera toxin underwent endocytosis in cisternae and vesicles of GERL (Golgi-Endoplasmic Reticulum-Lysosome). We suggest that GERL is the primary ercipieint of adsorptively endocytosed plasma membrane \"receptor\"-ligand complexes which are thus degraded or possibly reutilized (recycling). Wheat germ agglutinin-horseradish peroxidase conjugates used in vivo for studies of retrograde axonal transport were significantly more sensitive than free horseradish peroxidase.", "contents": "The use of lectins and cholera toxin for the detection of surface carbohydrates of cultured neurons and neuroblastoma. Conjugates of horseradish peroxidase with the lectins ricin (d-galactose), wheat germ agglutinin (N-acetylglucosamine), phytohemagglutinin (N-acetylgalactosamine), and with cholera toxin (GM1 ganglioside) were used for a cytochemical detection of corresponding termin al carbohydrates, or glycolipids on cell surfaces of cultured neurons and neuroblastoma cells. Cells were labeled at 4 degrees C with the above ligands and their adsorptive endocytosis was studied after incubations at 37 degrees C in a medium free of ligand. Peroxidase was detected by the method of Graham and Karnovsky (J. Histochem. Cytochem. 14:291, 1966). Lectins and cholera toxin underwent endocytosis in cisternae and vesicles of GERL (Golgi-Endoplasmic Reticulum-Lysosome). We suggest that GERL is the primary ercipieint of adsorptively endocytosed plasma membrane \"receptor\"-ligand complexes which are thus degraded or possibly reutilized (recycling). Wheat germ agglutinin-horseradish peroxidase conjugates used in vivo for studies of retrograde axonal transport were significantly more sensitive than free horseradish peroxidase."}
{"id": "PMID:479562", "title": "Subcellular localization of complex carbohydrates in rat macrophages and monocytes.", "content": "Methods for visualization of complex carbohydrates ultrastructurally were employed to study specific organelles of the rat monocyte and macrophage. Vicinal glycols of glycoconjugates were demonstrated with the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) postembedding sequence and acid groups were delineated by the dialyzed iron (DI) and high iron diamine (HID) preembedding techniques. Lysosomal bodies were generally found reactive with all three methods, although those of monocytes from the bone marrow and peripheral blood were notably lacking in acidic groups. The Golgi complex was consistently PA-TCH-SP-reactive, as were associated vesicles and occasional cisternal expansions, possibly related to GERL. Numerous cytoplasmic vesicles and small granulated structures and cisternae of the rough endoplasmic reticulum were also PA-TCH-SP-reactive.", "contents": "Subcellular localization of complex carbohydrates in rat macrophages and monocytes. Methods for visualization of complex carbohydrates ultrastructurally were employed to study specific organelles of the rat monocyte and macrophage. Vicinal glycols of glycoconjugates were demonstrated with the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) postembedding sequence and acid groups were delineated by the dialyzed iron (DI) and high iron diamine (HID) preembedding techniques. Lysosomal bodies were generally found reactive with all three methods, although those of monocytes from the bone marrow and peripheral blood were notably lacking in acidic groups. The Golgi complex was consistently PA-TCH-SP-reactive, as were associated vesicles and occasional cisternal expansions, possibly related to GERL. Numerous cytoplasmic vesicles and small granulated structures and cisternae of the rough endoplasmic reticulum were also PA-TCH-SP-reactive."}
{"id": "PMID:479563", "title": "Cytochemical characterization of secretory and cell surface glycoconjugates by light and electron microscopy.", "content": "Lectin methods have increased the capacity for histochemical characterization and differentiation of glycoproteins and have demonstrated, for example, greater reactivity of gastrointestinal than of respiratory tract secretions with the periodate-concanavalin A-horseradish peroxidase method for localizing mannose-rich glycoprotein. Application of a battery of ultrastructural cytochemical methods with specificity for the constituents characteristically present in the complex carbohydrates provides knowledge of the distribution of the various recognizable types of glycoconjugates in tissues and cells showing, for example, marked differences in glycoconjugates of the apical compared with the basolateral plasmalemma in a given cell type and differences between apical plasmalemmas or basement membranes of different cell types. Such information raises questions as to the biologic significance of the different complex carbohydrates in various sites and, hopefully, will lead to a clearer understanding of their physiologic roles.", "contents": "Cytochemical characterization of secretory and cell surface glycoconjugates by light and electron microscopy. Lectin methods have increased the capacity for histochemical characterization and differentiation of glycoproteins and have demonstrated, for example, greater reactivity of gastrointestinal than of respiratory tract secretions with the periodate-concanavalin A-horseradish peroxidase method for localizing mannose-rich glycoprotein. Application of a battery of ultrastructural cytochemical methods with specificity for the constituents characteristically present in the complex carbohydrates provides knowledge of the distribution of the various recognizable types of glycoconjugates in tissues and cells showing, for example, marked differences in glycoconjugates of the apical compared with the basolateral plasmalemma in a given cell type and differences between apical plasmalemmas or basement membranes of different cell types. Such information raises questions as to the biologic significance of the different complex carbohydrates in various sites and, hopefully, will lead to a clearer understanding of their physiologic roles."}
{"id": "PMID:479569", "title": "A mammary gland whole mount technique that preserves cell fine structure for electron miscroscopy.", "content": "A mammary gland whole mount technique has been developed that preserves cell fine structure and makes it possible to also examine the preparations by electron microscopy. The glands are placed on glass microscope slides, fixed in a paraformaldehyde-glutaraldehyde mixture, defatted in acetone, stained with 0.5% methylene blue (or trypan blue) in saline, and dehydrated in ethanol. They are evaluated and photographed in 100% ethanol. Then specific areas (i.e. containing small growths, tumors, or other lesions) are selected, excised and prepared for electroscopy. The ultrastructural preservation is good, organelles are evident and there is no observable dye precipitate. The only unusual finding is that cell membranes display a \"negative\" image.", "contents": "A mammary gland whole mount technique that preserves cell fine structure for electron miscroscopy. A mammary gland whole mount technique has been developed that preserves cell fine structure and makes it possible to also examine the preparations by electron microscopy. The glands are placed on glass microscope slides, fixed in a paraformaldehyde-glutaraldehyde mixture, defatted in acetone, stained with 0.5% methylene blue (or trypan blue) in saline, and dehydrated in ethanol. They are evaluated and photographed in 100% ethanol. Then specific areas (i.e. containing small growths, tumors, or other lesions) are selected, excised and prepared for electroscopy. The ultrastructural preservation is good, organelles are evident and there is no observable dye precipitate. The only unusual finding is that cell membranes display a \"negative\" image."}
{"id": "PMID:479571", "title": "Complement-mediated binding of immunoglobulins to some endocrine cells of the pancreas and gut.", "content": "Unspecific binding of immunoglobulins to gastrin, somatostain and glucagon cells of the gastrointestinal mucosa or pancreas has been found to occur through a nonantigen-antibody mechanism mediated by the C14 fraction of complement. The phenomenon represents an important drawback in hormone immunohistochemistry, which can be overcome by using complement deprived, highly dilute anti-hormone sera.", "contents": "Complement-mediated binding of immunoglobulins to some endocrine cells of the pancreas and gut. Unspecific binding of immunoglobulins to gastrin, somatostain and glucagon cells of the gastrointestinal mucosa or pancreas has been found to occur through a nonantigen-antibody mechanism mediated by the C14 fraction of complement. The phenomenon represents an important drawback in hormone immunohistochemistry, which can be overcome by using complement deprived, highly dilute anti-hormone sera."}
{"id": "PMID:479572", "title": "Innervation of the pancreas by substance P, enkephalin, vasoactive intestinal polypeptide and gastrin/CCK immunoractive nerves.", "content": "Immunocytochemical studies habe shown that many peptides which profoundly affect the endocrine and exocrine functions of the pancreas are localized to neurons. In the cat, such peptidergic nerves appear to innervate ganglia, islets and blood vessels of the pancreas, whereas their contributions to exocrine cells are minor. Our studies suggest that pancreatic ganglia represent one major site of action of the peptides and that, in addition, nerves containing the vasoactive intestinal polypeptide and gastrin/CCK-related peptides profoundly affect pancreatic blood flow and insulin secretion, respectively.", "contents": "Innervation of the pancreas by substance P, enkephalin, vasoactive intestinal polypeptide and gastrin/CCK immunoractive nerves. Immunocytochemical studies habe shown that many peptides which profoundly affect the endocrine and exocrine functions of the pancreas are localized to neurons. In the cat, such peptidergic nerves appear to innervate ganglia, islets and blood vessels of the pancreas, whereas their contributions to exocrine cells are minor. Our studies suggest that pancreatic ganglia represent one major site of action of the peptides and that, in addition, nerves containing the vasoactive intestinal polypeptide and gastrin/CCK-related peptides profoundly affect pancreatic blood flow and insulin secretion, respectively."}
{"id": "PMID:479573", "title": "Ultrastructural localization of neurophysin-like immunoreactivity in the rat posterior pituitary. An alternative method using frozen-dried tissue fixed with OsO4 vapor.", "content": "Electron microscopic identification of elements containing neurophysin-like immunoreactivity can be accomplished in rat posterior pituitary that has been frozen-dried and fixed with OsO4 vapor. Alternating serial ultrathin sections are placed on grids and glass slides. The sections on the slides are stained for neurophysin using immunofluorescence histochemistry, and the resultant images are superimposed on electron micrographs from the adjacent sections. The method provides several advantages for the localization of neuropeptide immunoreactivity in nervous tissue.", "contents": "Ultrastructural localization of neurophysin-like immunoreactivity in the rat posterior pituitary. An alternative method using frozen-dried tissue fixed with OsO4 vapor. Electron microscopic identification of elements containing neurophysin-like immunoreactivity can be accomplished in rat posterior pituitary that has been frozen-dried and fixed with OsO4 vapor. Alternating serial ultrathin sections are placed on grids and glass slides. The sections on the slides are stained for neurophysin using immunofluorescence histochemistry, and the resultant images are superimposed on electron micrographs from the adjacent sections. The method provides several advantages for the localization of neuropeptide immunoreactivity in nervous tissue."}
{"id": "PMID:479574", "title": "The subcommissural organ of submammalian vertebrates: a histochemical study.", "content": "Since morphological data do not allow to draw conclusions concerning the phyletic evolution of the subcommissural organ (SCO), a \"metabolic\" criterion is considered. Histoenzymology permits a comparison of metabolic activity in the subcommissural organ and that found within the ependyma. During the course of phyletic evolution, one notes various changes. Indeed, in lower vertebrates, the SCO presents a greater degree of enzyme activity than the ependyma while in birds the ependyma demonstrates a more intense activity than the SCO. The results are also compared with those previously reported in reptiles and mammals. In all vertebrates, glucid catabolism seems predominent in this areas.", "contents": "The subcommissural organ of submammalian vertebrates: a histochemical study. Since morphological data do not allow to draw conclusions concerning the phyletic evolution of the subcommissural organ (SCO), a \"metabolic\" criterion is considered. Histoenzymology permits a comparison of metabolic activity in the subcommissural organ and that found within the ependyma. During the course of phyletic evolution, one notes various changes. Indeed, in lower vertebrates, the SCO presents a greater degree of enzyme activity than the ependyma while in birds the ependyma demonstrates a more intense activity than the SCO. The results are also compared with those previously reported in reptiles and mammals. In all vertebrates, glucid catabolism seems predominent in this areas."}
{"id": "PMID:479575", "title": "Post-hatching growth and allometry of the teleost brain.", "content": "The growth of the brain in the rainbow trout follows an S curve: E = 0.04 t3 + 0.26 t2 - 0.06 t + 0.04 with t (time) in days and E (brain weight) in mg. The growth of the brain, relative to the body, can be given, after longarithmic transformation, by: Y = 0.011 + 0.835 X - 0.047 X2 where Y is log brain weight and X log body weight. This formula is consistent with that of every species after necessary changes of origin. The coefficients of allometry corresponding to this curve vary from 0.788 (body weight from 0.01 to 0.1 g) to 0.226 (body weight from 10 to 100 kg), the most common values varying between 0.507 (body weight from 10 to 100 g) and 0.414 (body weight from 100 g to 1 kg).", "contents": "Post-hatching growth and allometry of the teleost brain. The growth of the brain in the rainbow trout follows an S curve: E = 0.04 t3 + 0.26 t2 - 0.06 t + 0.04 with t (time) in days and E (brain weight) in mg. The growth of the brain, relative to the body, can be given, after longarithmic transformation, by: Y = 0.011 + 0.835 X - 0.047 X2 where Y is log brain weight and X log body weight. This formula is consistent with that of every species after necessary changes of origin. The coefficients of allometry corresponding to this curve vary from 0.788 (body weight from 0.01 to 0.1 g) to 0.226 (body weight from 10 to 100 kg), the most common values varying between 0.507 (body weight from 10 to 100 g) and 0.414 (body weight from 100 g to 1 kg)."}
{"id": "PMID:479577", "title": "Comparative study on the teleostean optic tectum. Lamination and cytoarchitecture.", "content": "Seventy-five species of teleosts were studied by a modified Bodian and the Golgi-Cox method to clarify certain relationship between habits and laminar formation in the optic tectum. The optic tectum of all species studied was divided into four layers (SM, 20 + SFGS, SGC + SAC and SPV) depending upon fiber connections with other areas, and the relative thickness of each layer was measured. All animals were classified into 8 groups (thick SM, thin SM, thick SO + SFGS, thin SO + SFGS, thick SGC + SAC, thick SPV, thin SPV and standard) based on relative thickness of each layer. The thick SM group has the large torus longitudinalis. The SO + SFGS group comprises diurnal, especially visually active fishes. As relative thickness of surface layers (SM and SO + SFGS) increases, the absolute value of whole layers becomes large. Aniamls with large relative thickness of the deepest layer (SPV) show the thin optic tectum. Seven types of cells, (a) pyramidal cell, (b) fusiform cell, (c) periventricular cell, (d) pyriform cell, (e) large multipolar cell, (f) horizontal cell and (g) small multipolar cell, were classified in Golgi-Cox preparations, and the extent of dendritic arborization was compared among the groups in relation to the thickness of each layer. Dendritis of pyramidal, fusiform and preiventricular neurons show varoius extents of arborization in proportion to thickness of layers where branch out. Other types of neuron do not conspicously vary in different groups. Animals belonging to the thick SPV group show poor dendritic arborization in all types of cells. These results were discussed from the ecological standpoint.", "contents": "Comparative study on the teleostean optic tectum. Lamination and cytoarchitecture. Seventy-five species of teleosts were studied by a modified Bodian and the Golgi-Cox method to clarify certain relationship between habits and laminar formation in the optic tectum. The optic tectum of all species studied was divided into four layers (SM, 20 + SFGS, SGC + SAC and SPV) depending upon fiber connections with other areas, and the relative thickness of each layer was measured. All animals were classified into 8 groups (thick SM, thin SM, thick SO + SFGS, thin SO + SFGS, thick SGC + SAC, thick SPV, thin SPV and standard) based on relative thickness of each layer. The thick SM group has the large torus longitudinalis. The SO + SFGS group comprises diurnal, especially visually active fishes. As relative thickness of surface layers (SM and SO + SFGS) increases, the absolute value of whole layers becomes large. Aniamls with large relative thickness of the deepest layer (SPV) show the thin optic tectum. Seven types of cells, (a) pyramidal cell, (b) fusiform cell, (c) periventricular cell, (d) pyriform cell, (e) large multipolar cell, (f) horizontal cell and (g) small multipolar cell, were classified in Golgi-Cox preparations, and the extent of dendritic arborization was compared among the groups in relation to the thickness of each layer. Dendritis of pyramidal, fusiform and preiventricular neurons show varoius extents of arborization in proportion to thickness of layers where branch out. Other types of neuron do not conspicously vary in different groups. Animals belonging to the thick SPV group show poor dendritic arborization in all types of cells. These results were discussed from the ecological standpoint."}
{"id": "PMID:479578", "title": "[Enzymatic chemoarchitecture of the isocortex of humans (area 10 of Brodmann)].", "content": "The distributions of some oxidoreductases, esterases and phosphatases were investigated in the area 10 (lobus frontalis cerebri) of old human brains by means of histoenzymatic methods. It was possible to observe a different spread of enzymatic activites of hydrolases and oxidoreductases in the several layers of human frontal cortex. The microphotometrical gradation of oxidoreductases was identical with the results of the most mammal brains with the exception of the alpha-glycerophosphate dehydrogenase. The enzyme products of this oxidoreductase show a increase in the lamina 6 and in the subcortex. The cause of the culmination is not jet clear.", "contents": "[Enzymatic chemoarchitecture of the isocortex of humans (area 10 of Brodmann)]. The distributions of some oxidoreductases, esterases and phosphatases were investigated in the area 10 (lobus frontalis cerebri) of old human brains by means of histoenzymatic methods. It was possible to observe a different spread of enzymatic activites of hydrolases and oxidoreductases in the several layers of human frontal cortex. The microphotometrical gradation of oxidoreductases was identical with the results of the most mammal brains with the exception of the alpha-glycerophosphate dehydrogenase. The enzyme products of this oxidoreductase show a increase in the lamina 6 and in the subcortex. The cause of the culmination is not jet clear."}
{"id": "PMID:479579", "title": "Catecholamine activity and infectious disease episodes.", "content": "The profile of 3-hydroxy-4-methoxy mandelic acid (VMA) excretion was studied in relation to reported acute infectious disease episodes. Daily VMA excretion levels and symptom reports were analyzed for a group of 47 volunteers over a four-week period. Results showed a tendency for elevated VMA levels to occur with greater frequency within three days prior to the onset of symptoms. These findings are interpreted as suggesting that elevated levels of catecholamine activity may increase susceptibility to disease by interfering with the immune response, and in the presence of an agent lead to an infectious disease episode.", "contents": "Catecholamine activity and infectious disease episodes. The profile of 3-hydroxy-4-methoxy mandelic acid (VMA) excretion was studied in relation to reported acute infectious disease episodes. Daily VMA excretion levels and symptom reports were analyzed for a group of 47 volunteers over a four-week period. Results showed a tendency for elevated VMA levels to occur with greater frequency within three days prior to the onset of symptoms. These findings are interpreted as suggesting that elevated levels of catecholamine activity may increase susceptibility to disease by interfering with the immune response, and in the presence of an agent lead to an infectious disease episode."}
{"id": "PMID:479580", "title": "Life change events and mental illness: an overview.", "content": "The use of recent life change measurement in the lives of persons developing mental disorders parallels the measurement of risk factors by epidemiologists to understand disease distribution. Examples are given of these parallels, along with critical commentary on methodological issues in life changes research. The principal studies documenting the association of recent life change and depression, schizophrenia, and neurosis are reviewed. Recent life changes appear to be an important element in explaining illness onset. Future advances in the area of life change and illness await the development of reliable and valid measures of an individual's stress tolerance characteristics, such as social support systems, psychological defenses, coping capabilities, and illness behavior tendencies.", "contents": "Life change events and mental illness: an overview. The use of recent life change measurement in the lives of persons developing mental disorders parallels the measurement of risk factors by epidemiologists to understand disease distribution. Examples are given of these parallels, along with critical commentary on methodological issues in life changes research. The principal studies documenting the association of recent life change and depression, schizophrenia, and neurosis are reviewed. Recent life changes appear to be an important element in explaining illness onset. Future advances in the area of life change and illness await the development of reliable and valid measures of an individual's stress tolerance characteristics, such as social support systems, psychological defenses, coping capabilities, and illness behavior tendencies."}
{"id": "PMID:479582", "title": "Life event classifications and the event-illness relationship.", "content": "Several theoretically important and distinct categories of life change are found in most life event scales. These categories can be organized in terms of at least three dimensions: the person's control over the event, the desirability of the event, and whether or not the independent variable of the event is confounded with the dependent variable of illness. It is important to separate conceptually and, to the extent possible, to distinguish empirically among events according to these dimensions, because several different models of the event-illness relationship are implied when events from several categories are combined. A secondary analysis of  recently published data shows that the kinds of events associated with illness are undesirable events within the subject's control. It may not be necessary to consider these dimensions in predicting illness, but the prevention and understanding of illness are furthered by their consideration.", "contents": "Life event classifications and the event-illness relationship. Several theoretically important and distinct categories of life change are found in most life event scales. These categories can be organized in terms of at least three dimensions: the person's control over the event, the desirability of the event, and whether or not the independent variable of the event is confounded with the dependent variable of illness. It is important to separate conceptually and, to the extent possible, to distinguish empirically among events according to these dimensions, because several different models of the event-illness relationship are implied when events from several categories are combined. A secondary analysis of  recently published data shows that the kinds of events associated with illness are undesirable events within the subject's control. It may not be necessary to consider these dimensions in predicting illness, but the prevention and understanding of illness are furthered by their consideration."}
{"id": "PMID:479584", "title": "Dietary fibre and the irritable bowel syndrome.", "content": "The dietary fibre intake of 25 patients with the irritable bowel syndrome was assessed by dietary recall over one week for the period before onset of symptoms, at diagnosis and after six months treatment with bran and a fibre-rich diet, and compared with controls matched for age and sex. Mean intake of patients fell from 20.2 g per day before onset of symptoms to 17.8 g at diagnosis (n.s.), when it was significantly less than the controls' intake of 22.6 g (P less than 0.01). The 20 patients who improved with treatment increased their intake from 18.9 g to 24.8 g (P less than 0.02); the five patients who did not improve did not increase their intake significantly (13.5 g to 15.6 g).", "contents": "Dietary fibre and the irritable bowel syndrome. The dietary fibre intake of 25 patients with the irritable bowel syndrome was assessed by dietary recall over one week for the period before onset of symptoms, at diagnosis and after six months treatment with bran and a fibre-rich diet, and compared with controls matched for age and sex. Mean intake of patients fell from 20.2 g per day before onset of symptoms to 17.8 g at diagnosis (n.s.), when it was significantly less than the controls' intake of 22.6 g (P less than 0.01). The 20 patients who improved with treatment increased their intake from 18.9 g to 24.8 g (P less than 0.02); the five patients who did not improve did not increase their intake significantly (13.5 g to 15.6 g)."}
{"id": "PMID:479585", "title": "Zinc, copper, iron and lead in scalp hair of students and non-student adults in Oxford.", "content": "Scalp hair samples contributed by 215 students and non-student adults in Oxford were washed, ashed and analysed by atomic absorption spectrophotometry for zinc, iron, copper and lead. The overall results were within the ranges reported from similar investigations carried out elsewhere and are what might be expected with normal, healthy subjects. However, examination of the results for different groups showed some significant differences between levels of the nutrient metals in student and non-student hair. Results for lead indicate a low level of environmental contamination for all subjects.", "contents": "Zinc, copper, iron and lead in scalp hair of students and non-student adults in Oxford. Scalp hair samples contributed by 215 students and non-student adults in Oxford were washed, ashed and analysed by atomic absorption spectrophotometry for zinc, iron, copper and lead. The overall results were within the ranges reported from similar investigations carried out elsewhere and are what might be expected with normal, healthy subjects. However, examination of the results for different groups showed some significant differences between levels of the nutrient metals in student and non-student hair. Results for lead indicate a low level of environmental contamination for all subjects."}
{"id": "PMID:479586", "title": "High-fibre foods: a feasibility study using guar gum.", "content": "Guar gum at different concentrations was incorporated into foods from six groups to establish some principles to aid the production of high-viscosity therapeutic foods. The amounts of gum that could be incorporated into a food, without major loss of acceptability, were higher in products with low final water content than those with high final water content. The domestic preparation of these recipes should be possible provided that recipe modifications are carefully followed.", "contents": "High-fibre foods: a feasibility study using guar gum. Guar gum at different concentrations was incorporated into foods from six groups to establish some principles to aid the production of high-viscosity therapeutic foods. The amounts of gum that could be incorporated into a food, without major loss of acceptability, were higher in products with low final water content than those with high final water content. The domestic preparation of these recipes should be possible provided that recipe modifications are carefully followed."}
{"id": "PMID:479587", "title": "Working as an applied nutritionist in Third World development.", "content": "The nature of development programmes is considered and employment opportunities in the Third World are briefly summarised. The qualities needed for dietetic educational proficiency in an alien culture are discussed. The assessment of cultural influences should be applied to dietetic teaching situations in Britain as well as to overseas work.", "contents": "Working as an applied nutritionist in Third World development. The nature of development programmes is considered and employment opportunities in the Third World are briefly summarised. The qualities needed for dietetic educational proficiency in an alien culture are discussed. The assessment of cultural influences should be applied to dietetic teaching situations in Britain as well as to overseas work."}
{"id": "PMID:479588", "title": "Creamatocrit procedure versus triglyceride analysis: a comparison of methods for the determination of human milk fat in epidemiological studies.", "content": "A comparison is made of triglyceride analysis and the creamatocrit procedure to determine their usefulness as methods for measuring the fat content of human milk. Triglyceride analysis is shown to be the more precise technique but, for use in the field, the creamatocrit method has the advantage of simplicity.", "contents": "Creamatocrit procedure versus triglyceride analysis: a comparison of methods for the determination of human milk fat in epidemiological studies. A comparison is made of triglyceride analysis and the creamatocrit procedure to determine their usefulness as methods for measuring the fat content of human milk. Triglyceride analysis is shown to be the more precise technique but, for use in the field, the creamatocrit method has the advantage of simplicity."}
{"id": "PMID:479591", "title": "Antibody-mediated immunosuppression of a cytotoxic cell response not involving a simple antigen-masking mechanism.", "content": "An in vitro cytotoxic cell response against alloantigens was inhibited by antibody directed against the alloantigens and also by anti-trinitrophenyl antibody provided that the allogeneic stimulator cells were modified with trinitrophenyl. Inhibition of an anti-allogeneic cytotoxic cell response against trinitrophenyl-coupled allogeneic stimulator cells by anti-trinitrophenyl antibody is dependent upon the intact Fc portion of inhibitory antibody. The magnitude of the difference between intact and F(ab')2 anti-trinitrophenyl antibody in immunosuppressive activity suggests that the Fc portion emits negative signals rather than simply adding to a steric hindrance effect.", "contents": "Antibody-mediated immunosuppression of a cytotoxic cell response not involving a simple antigen-masking mechanism. An in vitro cytotoxic cell response against alloantigens was inhibited by antibody directed against the alloantigens and also by anti-trinitrophenyl antibody provided that the allogeneic stimulator cells were modified with trinitrophenyl. Inhibition of an anti-allogeneic cytotoxic cell response against trinitrophenyl-coupled allogeneic stimulator cells by anti-trinitrophenyl antibody is dependent upon the intact Fc portion of inhibitory antibody. The magnitude of the difference between intact and F(ab')2 anti-trinitrophenyl antibody in immunosuppressive activity suggests that the Fc portion emits negative signals rather than simply adding to a steric hindrance effect."}
{"id": "PMID:479593", "title": "Regulation of contact sensitivity to DNFB in the mouse: effects of adult thymectomy and thymic factor.", "content": "The contact sensitivity response to DNFB is decreased after adult thymectomy (ATX). This response decreases to 50% of the control response of normal age-matched mice as soon as 3 weeks after ATX and is not further depressed 9 to 16 weeks after ATX. These results suggest that two T cell subsets of different lifespan are involved in the anti-DNFB response. A circulating thymic factor (FTS) is able to restore the contact sensitivity response to DNFB when injected 3 to 9 weeks after ATX but not 16 weeks later. By contrast, FTS has a depressive effect on the contact sensitivity response to DNFB of normal mice through a cyclophosphamide-sensitive T cell subset. These results suggest that FTS regulates DNFB contact sensitivity by acting on a cyclophosphamide-sensitive T cell subset, still present 9 weeks after ATX but absent after 16 weeks. Thus although the T cell defect, causing a depression of the contact sensitivity reaction to DNFB is quantitatively similar 3 and 16 weeks after ATX, its nature is probably different.", "contents": "Regulation of contact sensitivity to DNFB in the mouse: effects of adult thymectomy and thymic factor. The contact sensitivity response to DNFB is decreased after adult thymectomy (ATX). This response decreases to 50% of the control response of normal age-matched mice as soon as 3 weeks after ATX and is not further depressed 9 to 16 weeks after ATX. These results suggest that two T cell subsets of different lifespan are involved in the anti-DNFB response. A circulating thymic factor (FTS) is able to restore the contact sensitivity response to DNFB when injected 3 to 9 weeks after ATX but not 16 weeks later. By contrast, FTS has a depressive effect on the contact sensitivity response to DNFB of normal mice through a cyclophosphamide-sensitive T cell subset. These results suggest that FTS regulates DNFB contact sensitivity by acting on a cyclophosphamide-sensitive T cell subset, still present 9 weeks after ATX but absent after 16 weeks. Thus although the T cell defect, causing a depression of the contact sensitivity reaction to DNFB is quantitatively similar 3 and 16 weeks after ATX, its nature is probably different."}
{"id": "PMID:479596", "title": "Organ and isotype distribution of plasma cells producing specific antibody after oral immunization: evidence for a generalized secretory immune system.", "content": "Mice were induced to produce IgA antibodies against ferritin after oral immunization. Such antibodies were detected by immunofluorescence in plasma cells in the intestinal mucosa as well as in secretory sites located elsewhere, such as the lactating mammary gland, salivary gland, and respiratory tract. The observation suggested that cells immunized locally via the gut could home to distant secretory sites. To confirm this hypothesis, lymphocyte transfer studies were done with mesenteric node (MN) versus peripheral node (PN) cells from orally immunized donors into nonimmunized recipients. IgA anti-ferritin cells from MN homed to exocrine targets, whereas IgM and IgG anti-ferritin cells homed to PN. The findings overall support the concept of a generalized and interrelated secretory immune system.", "contents": "Organ and isotype distribution of plasma cells producing specific antibody after oral immunization: evidence for a generalized secretory immune system. Mice were induced to produce IgA antibodies against ferritin after oral immunization. Such antibodies were detected by immunofluorescence in plasma cells in the intestinal mucosa as well as in secretory sites located elsewhere, such as the lactating mammary gland, salivary gland, and respiratory tract. The observation suggested that cells immunized locally via the gut could home to distant secretory sites. To confirm this hypothesis, lymphocyte transfer studies were done with mesenteric node (MN) versus peripheral node (PN) cells from orally immunized donors into nonimmunized recipients. IgA anti-ferritin cells from MN homed to exocrine targets, whereas IgM and IgG anti-ferritin cells homed to PN. The findings overall support the concept of a generalized and interrelated secretory immune system."}
{"id": "PMID:479598", "title": "Rapid magnetic purification of rosette-forming lymphocytes.", "content": "High gradient magnetic separation, which as previously been shown effective in extracting erythrocytes from a flowing cell suspension, has been used to separate rosetted and unrosetted human peripheral blood lymphocytes. The hemoglobin in the sheep red cells used to form rosettes was first oxidizied to the paramagnetic methemoglobin form. Samples of 50 x 10(6) lymphocytes could be processed in 10 min under sterile conditions with greater than 90% purity of the rosetted cell fraction and maintenance of T cell function in mixed lymphocyte cultures.", "contents": "Rapid magnetic purification of rosette-forming lymphocytes. High gradient magnetic separation, which as previously been shown effective in extracting erythrocytes from a flowing cell suspension, has been used to separate rosetted and unrosetted human peripheral blood lymphocytes. The hemoglobin in the sheep red cells used to form rosettes was first oxidizied to the paramagnetic methemoglobin form. Samples of 50 x 10(6) lymphocytes could be processed in 10 min under sterile conditions with greater than 90% purity of the rosetted cell fraction and maintenance of T cell function in mixed lymphocyte cultures."}
{"id": "PMID:479599", "title": "Measurement of cell-mediated inflammation in experimental murine autoimmune encephalomyelitis by radioisotopic labeling.", "content": "A radioisotopic index test was used to detect that time of onset and intensity of cell-mediated immune inflammation of experimental autoimmune encephalomyelitis (EAE) in mice. Mice were tested at various time intervals after an encephalitogenic immunization with mouse spinal cord to homogenate for delayed-type hypersensitivity (DTH) to myelin basic protein (MBP) by intradermal challenge with antigen in the ear pinna. After 25 hr, the intensity of DTH was measured by 125I-radiometry which depends upon the migration of 125I-UdR radiolabeled mononuclear cells into the antigen depot. Cells reactive to MBP were detected by the ear assay as early as 7 days after the initial encephalitogenic sensitization. The degree of cell-mediated immune inflammation in the brain and spinal cord during the evolution of EAE was also measured by a radioisotopic technique; increased 125I-UdR uptake could be detected in the brain 3 to 4 days before the onset of signs of EAE at days 11 to 12, whereas 125I-UdR in the spinal cord was detected only 1 day before, or concomitant with, the onset of signs of EAE. Both, or concomitant with, the onset of signs of EAE. Both the \"ear\" and \"organ\" radiometric index tests are useful in measuring the degree of cell-mediated inflammation in EAE, and supplement routine histopathological and observational assessments.", "contents": "Measurement of cell-mediated inflammation in experimental murine autoimmune encephalomyelitis by radioisotopic labeling. A radioisotopic index test was used to detect that time of onset and intensity of cell-mediated immune inflammation of experimental autoimmune encephalomyelitis (EAE) in mice. Mice were tested at various time intervals after an encephalitogenic immunization with mouse spinal cord to homogenate for delayed-type hypersensitivity (DTH) to myelin basic protein (MBP) by intradermal challenge with antigen in the ear pinna. After 25 hr, the intensity of DTH was measured by 125I-radiometry which depends upon the migration of 125I-UdR radiolabeled mononuclear cells into the antigen depot. Cells reactive to MBP were detected by the ear assay as early as 7 days after the initial encephalitogenic sensitization. The degree of cell-mediated immune inflammation in the brain and spinal cord during the evolution of EAE was also measured by a radioisotopic technique; increased 125I-UdR uptake could be detected in the brain 3 to 4 days before the onset of signs of EAE at days 11 to 12, whereas 125I-UdR in the spinal cord was detected only 1 day before, or concomitant with, the onset of signs of EAE. Both, or concomitant with, the onset of signs of EAE. Both the \"ear\" and \"organ\" radiometric index tests are useful in measuring the degree of cell-mediated inflammation in EAE, and supplement routine histopathological and observational assessments."}
{"id": "PMID:479600", "title": "Genetic control of sensitivity to Moloney leukemia virus in mice. II. Mapping of three resistant genes within the H-2 complex.", "content": "The level of viremia and the appearance of leukemias were studied after inoculation wtih Moloney leukemia virus (M-MuLV) in different H-2 congenic strains of mice. The viremia was regularly measured on individual mice with a radioimmunoassay of the major internal virion component p30. Three genes within the major histocompatibility complex controlled the level of circulating virus. Two of them, called Rmv.1 and Rmv.2, appear to be located in the I region, respectively, in the IA, and the IC-S or G regions. The third gene, Rmv.3, was mapped to the D end of the complex in the D or T region. Crosses between resistant and sensitive strans demonstrated that the H-2 associated resistance was inherited as a dominant or semi-dominant Mendelian trait. Rmv.1, Rmv.2, and Rmv.3 were shown to complement for resistance in trans when the hybrids between sensitive strains were examined. A good correlation was found between viremia and the appearance of leukemias, the most viremic strains being also the most leukemic. Nevertheless, additional non-H-2 genes must control viremia and/or the appearance of leukemia since, despite high levels of viremia, some sensitive strains do not become leukemic.", "contents": "Genetic control of sensitivity to Moloney leukemia virus in mice. II. Mapping of three resistant genes within the H-2 complex. The level of viremia and the appearance of leukemias were studied after inoculation wtih Moloney leukemia virus (M-MuLV) in different H-2 congenic strains of mice. The viremia was regularly measured on individual mice with a radioimmunoassay of the major internal virion component p30. Three genes within the major histocompatibility complex controlled the level of circulating virus. Two of them, called Rmv.1 and Rmv.2, appear to be located in the I region, respectively, in the IA, and the IC-S or G regions. The third gene, Rmv.3, was mapped to the D end of the complex in the D or T region. Crosses between resistant and sensitive strans demonstrated that the H-2 associated resistance was inherited as a dominant or semi-dominant Mendelian trait. Rmv.1, Rmv.2, and Rmv.3 were shown to complement for resistance in trans when the hybrids between sensitive strains were examined. A good correlation was found between viremia and the appearance of leukemias, the most viremic strains being also the most leukemic. Nevertheless, additional non-H-2 genes must control viremia and/or the appearance of leukemia since, despite high levels of viremia, some sensitive strains do not become leukemic."}
{"id": "PMID:479601", "title": "Histocompatibilty-linked susceptibility for hepatospleenomegaly in human schistosomiasis mansoni.", "content": "In schistosomiasis mansoni, the pathogenesis of hepatosplenic disease has been shown to be due primarily to immune mechanisms. The present study was designed to examine the relationship between the development of schistosomal hepatosplenomegaly in Egyptian school children and the HLA antigens. Two groups of schistosome-infected children with similar fecal egg counts were examined: one group (23 children) had no clinically demonstrable hepatosplenomegaly whereas all the children (28) in the second group suffered from liver enlargement. Furthermore, 13 of the 28 individuals in the latter group had splenomegaly as well. Our results show that hepatosplenomegaly was related to the presence of two HLA antigens: HLA AI and B5. The average relative risk of developing hepatomegaly is 29 for HLA AI and 18.9 for 55.6. Furthermore, the severity of hepatomegaly was correlated with the presence of these two HLA antigens. These findings represent a step toward elucidating the factors controlling the pathogenic mechanisms in human schistosomiasis mansoni.", "contents": "Histocompatibilty-linked susceptibility for hepatospleenomegaly in human schistosomiasis mansoni. In schistosomiasis mansoni, the pathogenesis of hepatosplenic disease has been shown to be due primarily to immune mechanisms. The present study was designed to examine the relationship between the development of schistosomal hepatosplenomegaly in Egyptian school children and the HLA antigens. Two groups of schistosome-infected children with similar fecal egg counts were examined: one group (23 children) had no clinically demonstrable hepatosplenomegaly whereas all the children (28) in the second group suffered from liver enlargement. Furthermore, 13 of the 28 individuals in the latter group had splenomegaly as well. Our results show that hepatosplenomegaly was related to the presence of two HLA antigens: HLA AI and B5. The average relative risk of developing hepatomegaly is 29 for HLA AI and 18.9 for 55.6. Furthermore, the severity of hepatomegaly was correlated with the presence of these two HLA antigens. These findings represent a step toward elucidating the factors controlling the pathogenic mechanisms in human schistosomiasis mansoni."}
{"id": "PMID:479604", "title": "Promonocytes have the functional characteristics of natural killer cells.", "content": "Promonocytes isolated from a 5-day-old L-fibroblast-conditioned liquid bone marrow culture show strong NK cell cytotoxicity. They kill YAC target cells in a short-term 125IUDR-release assay whereas P815 targets are unaffected. This NK-like cytotoxicity is enhanced in the presence of interferon preparations. Morphologically, these promonocytes resemble a medium size lymphocyte with a high nucleus to cytoplasma ratio, they are nonadherent, nonphagocytic, and negative in nonspecific esterase staining. Promonocytes are precursor cells from macrophages, which have not yet developed the typical macrophage criteria. Within 24 to 48 hr they mature to adherent macrophages. We have shown previously, that the same promonocytes have the capacity to perform K cell killing of antibody-coated tumor target cells. The cytotoxic effector functions of promonocytes are abolished when the cells are treated with the alloantimacrophage serum Mphi 1.2 plus rabbit C. The relationship or similarity between K cells, NK cells and promonocytes is discussed.", "contents": "Promonocytes have the functional characteristics of natural killer cells. Promonocytes isolated from a 5-day-old L-fibroblast-conditioned liquid bone marrow culture show strong NK cell cytotoxicity. They kill YAC target cells in a short-term 125IUDR-release assay whereas P815 targets are unaffected. This NK-like cytotoxicity is enhanced in the presence of interferon preparations. Morphologically, these promonocytes resemble a medium size lymphocyte with a high nucleus to cytoplasma ratio, they are nonadherent, nonphagocytic, and negative in nonspecific esterase staining. Promonocytes are precursor cells from macrophages, which have not yet developed the typical macrophage criteria. Within 24 to 48 hr they mature to adherent macrophages. We have shown previously, that the same promonocytes have the capacity to perform K cell killing of antibody-coated tumor target cells. The cytotoxic effector functions of promonocytes are abolished when the cells are treated with the alloantimacrophage serum Mphi 1.2 plus rabbit C. The relationship or similarity between K cells, NK cells and promonocytes is discussed."}
{"id": "PMID:479605", "title": "Antibody-dependent cellular cytotoxicity effector cell capability among normal individuals.", "content": "Nonadherent peripheral blood lymphocytes (PBL) from 110 normal individuals were evaluated for their capability to mediate antibody-dependent cellular cytotoxicity (ADCC) against rabbit antibody-sensitized chicken erythrocytes (CRBC). Titration of PBL against a constant number of chromium-51 labeled CRBC allowed for construction of dose response cytotoxicity curves where percent chromium release was plotted against the number of PBL. From these curves the maximum degree of lymphocyte-mediated cytotoxicity and the number of lymphoid cells required for 50% of the maximum lymphocyte-mediated cytotoxicity were determined. The results suggested that in this system the cytotoxic capability of PBL from normal individuals was independent of the age and sex of the donor. The cytotoxic capability of PBL was also found to be constant in individuals tested sequentially. However, significant differences were found to exist between different individuals in their capability of mediating ADCC in this system.", "contents": "Antibody-dependent cellular cytotoxicity effector cell capability among normal individuals. Nonadherent peripheral blood lymphocytes (PBL) from 110 normal individuals were evaluated for their capability to mediate antibody-dependent cellular cytotoxicity (ADCC) against rabbit antibody-sensitized chicken erythrocytes (CRBC). Titration of PBL against a constant number of chromium-51 labeled CRBC allowed for construction of dose response cytotoxicity curves where percent chromium release was plotted against the number of PBL. From these curves the maximum degree of lymphocyte-mediated cytotoxicity and the number of lymphoid cells required for 50% of the maximum lymphocyte-mediated cytotoxicity were determined. The results suggested that in this system the cytotoxic capability of PBL from normal individuals was independent of the age and sex of the donor. The cytotoxic capability of PBL was also found to be constant in individuals tested sequentially. However, significant differences were found to exist between different individuals in their capability of mediating ADCC in this system."}
{"id": "PMID:479606", "title": "Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays.", "content": "Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (greater than 10(6) cpm/microgram) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.", "contents": "Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays. Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (greater than 10(6) cpm/microgram) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays."}
{"id": "PMID:479607", "title": "Normal immunosuppressive protein purification and quantitative estimation experiments.", "content": "An improved method for the preparation and purification of normal immunosuppressive protein (NIP) is described. The purified material has a molecular weight between 10,000 and 25,000. Its biological and serological activity is approximately 10--20 times higher than that of the crude fraction. An antibody to normal immunosuppressive protein prepared in rabbits made the quantitative estimation of NIP by a haemaggluination inhibition test possible. Similarly, a very sensitive assay for the quantitative determination of NIP by its inhibitory effect on the proliferation of EL-4 tumor cells is also described. Eluates prepared from polyacrylamide gels were active in inhibiting EL-4 tumor cell proliferation and neutralized the anti-NIP activity in the haemagglutination inhibition test.", "contents": "Normal immunosuppressive protein purification and quantitative estimation experiments. An improved method for the preparation and purification of normal immunosuppressive protein (NIP) is described. The purified material has a molecular weight between 10,000 and 25,000. Its biological and serological activity is approximately 10--20 times higher than that of the crude fraction. An antibody to normal immunosuppressive protein prepared in rabbits made the quantitative estimation of NIP by a haemaggluination inhibition test possible. Similarly, a very sensitive assay for the quantitative determination of NIP by its inhibitory effect on the proliferation of EL-4 tumor cells is also described. Eluates prepared from polyacrylamide gels were active in inhibiting EL-4 tumor cell proliferation and neutralized the anti-NIP activity in the haemagglutination inhibition test."}
{"id": "PMID:479608", "title": "ACDAS: an automated chemotaxis data acquisition system.", "content": "An automated system (ACDAS) for rapid collection and analysis of data from leading front chemotaxis assays is described. ACDAS automatically computes (1) net cell migration from operator-entered end point signals and (2) means and standard errors of the mean from data sets of user-determined size. ACDAS reduces data collecting and processing time by more than 50% compared to manual methods. Other applications of ACDAS are discussed.", "contents": "ACDAS: an automated chemotaxis data acquisition system. An automated system (ACDAS) for rapid collection and analysis of data from leading front chemotaxis assays is described. ACDAS automatically computes (1) net cell migration from operator-entered end point signals and (2) means and standard errors of the mean from data sets of user-determined size. ACDAS reduces data collecting and processing time by more than 50% compared to manual methods. Other applications of ACDAS are discussed."}
{"id": "PMID:479609", "title": "Binding of ovalbumin to mouse spleen cells with and without carbodiimide.", "content": "We studied the binding of ovalbumin (OVA) to mouse spleen cells. In the presence or absence of carbodiimide (ECDI), uptake increased with greater cell numbers, increasing OVA concentration and increasing time up to 60 min. Between 60 and 210 microgram OVA could be coupled with ECDI to 2 X 10(8) cells. In the absence of ECDI, however, OVA uptake was still appreciable, but appeared to be of low avidity as it did not occur in the presence of competing fetal calf serum. These experiments provide a basis for the use of protein coupled to autologous cells in the induction of immunologic tolerance.", "contents": "Binding of ovalbumin to mouse spleen cells with and without carbodiimide. We studied the binding of ovalbumin (OVA) to mouse spleen cells. In the presence or absence of carbodiimide (ECDI), uptake increased with greater cell numbers, increasing OVA concentration and increasing time up to 60 min. Between 60 and 210 microgram OVA could be coupled with ECDI to 2 X 10(8) cells. In the absence of ECDI, however, OVA uptake was still appreciable, but appeared to be of low avidity as it did not occur in the presence of competing fetal calf serum. These experiments provide a basis for the use of protein coupled to autologous cells in the induction of immunologic tolerance."}
{"id": "PMID:479610", "title": "Detection of human histocompatibility (HLA) antigens with an indirect rosette microassay.", "content": "An indirect rosette assay which utilizes erythrocytes coupled with purified antiimmunoglobulin (Ig) antibodies was modified into a microassay for detecting HLA allo-and xenoantibodies. The test, which is performed in microtiter plates, is specific, reproducible and can handle large numbers of samples. As an inhibition assay the test can detect HLA antibodies even if mixed with antibodies to other cell surface structures. The rosette microassay is at least 10 times more sensitive than the complement-dependent microcytotoxic test and can use target cells which exhibit low viability or abnormal susceptibility to lysis.", "contents": "Detection of human histocompatibility (HLA) antigens with an indirect rosette microassay. An indirect rosette assay which utilizes erythrocytes coupled with purified antiimmunoglobulin (Ig) antibodies was modified into a microassay for detecting HLA allo-and xenoantibodies. The test, which is performed in microtiter plates, is specific, reproducible and can handle large numbers of samples. As an inhibition assay the test can detect HLA antibodies even if mixed with antibodies to other cell surface structures. The rosette microassay is at least 10 times more sensitive than the complement-dependent microcytotoxic test and can use target cells which exhibit low viability or abnormal susceptibility to lysis."}
{"id": "PMID:479611", "title": "A new method for semi-automated quantitation of E-rosettes using a particle size analyser (Coulter Channelyzer).", "content": "Visual counts of the number of SRBC attached to individual lymphocytes in E-rosette preparations from 3 healthy young adults were made by 3 experienced observers: from these data the size distribution profile of particles in each E-rosette preparation was calculated. In parallel experiments the size distribution profile was measured directly with a particle size analyser (Coulter Counter and Channelyzer): in every case the observed profile lay within the 95% confidence band around the reconstructed profiles. It was concluded that the Coulter Channelyzer profile is an accurate measurement of the E-rosette size distribution and that particle sizing could provide a reasonable basis for semi-automated tests for E-rosettes. A mathematical model has been developed by which the percentage of lymphocytes forming E-rosettes, the distribution of number of attached SRBC and the average avidity of the lymphocyte receptor for SRBC could be deduced from the size-distribution profiles. The method has a high degree of precision, and a good correlation (P less than 0.01) was obtained when the percentage of E-rosettes (defined for this purpose as having three or more attached SRBC) obtained from the new method was compared with results from standard light microscopy.", "contents": "A new method for semi-automated quantitation of E-rosettes using a particle size analyser (Coulter Channelyzer). Visual counts of the number of SRBC attached to individual lymphocytes in E-rosette preparations from 3 healthy young adults were made by 3 experienced observers: from these data the size distribution profile of particles in each E-rosette preparation was calculated. In parallel experiments the size distribution profile was measured directly with a particle size analyser (Coulter Counter and Channelyzer): in every case the observed profile lay within the 95% confidence band around the reconstructed profiles. It was concluded that the Coulter Channelyzer profile is an accurate measurement of the E-rosette size distribution and that particle sizing could provide a reasonable basis for semi-automated tests for E-rosettes. A mathematical model has been developed by which the percentage of lymphocytes forming E-rosettes, the distribution of number of attached SRBC and the average avidity of the lymphocyte receptor for SRBC could be deduced from the size-distribution profiles. The method has a high degree of precision, and a good correlation (P less than 0.01) was obtained when the percentage of E-rosettes (defined for this purpose as having three or more attached SRBC) obtained from the new method was compared with results from standard light microscopy."}
{"id": "PMID:479612", "title": "A simple cytochemical method for distinguishing EAC rosettes formed by lymphocytes and monocytes.", "content": "A simple cytochemical method for EAC rosettes in suspension is described which allows an easy distinction between rosettes formed by lymphocytes and monocytes. The method is based on the fact that monocytes, in contrast to lymphocytes, possess a specific cytoplasmic esterase.", "contents": "A simple cytochemical method for distinguishing EAC rosettes formed by lymphocytes and monocytes. A simple cytochemical method for EAC rosettes in suspension is described which allows an easy distinction between rosettes formed by lymphocytes and monocytes. The method is based on the fact that monocytes, in contrast to lymphocytes, possess a specific cytoplasmic esterase."}
{"id": "PMID:479613", "title": "Induction of specific anti-actin antibodies and their measurement by ELISA technique.", "content": "Using the ELISA technique we have been able to quantify antibodies directed against actin and to follow the kinetics of antibody production. Specific anti-actin antisera have been raised in rabbits by immunization with chemically modified white muscle rabbit actin. Two or three dinitrophenyl groups linked per actin molecule were sufficient to break natural tolerance, while linkage of three phosphorylcholine groups to actin was not.", "contents": "Induction of specific anti-actin antibodies and their measurement by ELISA technique. Using the ELISA technique we have been able to quantify antibodies directed against actin and to follow the kinetics of antibody production. Specific anti-actin antisera have been raised in rabbits by immunization with chemically modified white muscle rabbit actin. Two or three dinitrophenyl groups linked per actin molecule were sufficient to break natural tolerance, while linkage of three phosphorylcholine groups to actin was not."}
{"id": "PMID:479614", "title": "An enzyme-linked immunosorbent assay for antibodies to native and denatured DNA.", "content": "A modification of the enzyme-linked immunosorbent assay (ELISA) is described, that permits determination of antibodies to native DNA (nDNA). The same approach can be used to measure antibodies to denatured DNA (dDNA). Poor binding of nDNA to the polystyrene solid phase has presented difficulties in using the ELISA method for assaying anti-nDNA activity (Engvall, 1976), but we find that precoating of the solid phase with protamine sulfate circumvents this problem. Assays for anti-dDNA are also enhanced by the use of protamine sulfate coated tubes. We have used the ELISA method to assay 15 SLE and 27 non-SLE sera for anti-nDNA and anti-dDNA activity. The results are compared with those obtained using the GF/A glass fiber filter assay, previously described by Lewis et al. (1973).", "contents": "An enzyme-linked immunosorbent assay for antibodies to native and denatured DNA. A modification of the enzyme-linked immunosorbent assay (ELISA) is described, that permits determination of antibodies to native DNA (nDNA). The same approach can be used to measure antibodies to denatured DNA (dDNA). Poor binding of nDNA to the polystyrene solid phase has presented difficulties in using the ELISA method for assaying anti-nDNA activity (Engvall, 1976), but we find that precoating of the solid phase with protamine sulfate circumvents this problem. Assays for anti-dDNA are also enhanced by the use of protamine sulfate coated tubes. We have used the ELISA method to assay 15 SLE and 27 non-SLE sera for anti-nDNA and anti-dDNA activity. The results are compared with those obtained using the GF/A glass fiber filter assay, previously described by Lewis et al. (1973)."}
{"id": "PMID:479615", "title": "A radioisotopic technique for measuring the mononuclear inflammatory response in Sindbis virus-induced encephalitis of mice.", "content": "The mononuclear inflammation occurring in Sindbis virus-induced encephalitis of mice has been measured by means of pulse labelling with [125I]UdR at a defined time after infection. The method should be applicable to other studies of encephalitis and of mononuclear cellular infiltration occurring in other organs as a result of disease or infection.", "contents": "A radioisotopic technique for measuring the mononuclear inflammatory response in Sindbis virus-induced encephalitis of mice. The mononuclear inflammation occurring in Sindbis virus-induced encephalitis of mice has been measured by means of pulse labelling with [125I]UdR at a defined time after infection. The method should be applicable to other studies of encephalitis and of mononuclear cellular infiltration occurring in other organs as a result of disease or infection."}
{"id": "PMID:479616", "title": "A new gradient former and a simplified procedure for zonal centrifugation of immune complexes.", "content": "A new gradient former for production of optimized sucrose gradients for B-XIV zonal rotors is described. Optimized gradients are gradients with an initial shelf to provide capacity and a shape that ensures isokinetic sedimentation conditions. By exhaustive computer calculations it has been found that optimized gradients can be produced by a very simple two-compartment gradient former. The design is given. Moreover, graphs for choice of gradient strength and run duration for preparative and analytical runs of samples containing soluble immune complexes are calculated. The new gradient former allows the use of a very simple procedure for zonal centrifugation.", "contents": "A new gradient former and a simplified procedure for zonal centrifugation of immune complexes. A new gradient former for production of optimized sucrose gradients for B-XIV zonal rotors is described. Optimized gradients are gradients with an initial shelf to provide capacity and a shape that ensures isokinetic sedimentation conditions. By exhaustive computer calculations it has been found that optimized gradients can be produced by a very simple two-compartment gradient former. The design is given. Moreover, graphs for choice of gradient strength and run duration for preparative and analytical runs of samples containing soluble immune complexes are calculated. The new gradient former allows the use of a very simple procedure for zonal centrifugation."}
{"id": "PMID:479617", "title": "Radioimmunoassay for pyrazolone derivatives.", "content": "The determination of pyrazolone derivatives by radioimmunoassay has been reported. Anti-antipyrine antisera were obtained by repeated immunization of rabbits with 4-azoantipyrine-conjugated bovine serum albumin. The level at least as low as 1.0 ng of antipyrine could be detected by this procedure. Various substituents on the carbon 4 position of the pyrazolone ring as well as the lack of methyl group on the nitrogen 2 position decreased the affinity for the antibody, and the antibody showed no cross-reaction with any pyrazolidine derivatives.", "contents": "Radioimmunoassay for pyrazolone derivatives. The determination of pyrazolone derivatives by radioimmunoassay has been reported. Anti-antipyrine antisera were obtained by repeated immunization of rabbits with 4-azoantipyrine-conjugated bovine serum albumin. The level at least as low as 1.0 ng of antipyrine could be detected by this procedure. Various substituents on the carbon 4 position of the pyrazolone ring as well as the lack of methyl group on the nitrogen 2 position decreased the affinity for the antibody, and the antibody showed no cross-reaction with any pyrazolidine derivatives."}
{"id": "PMID:479618", "title": "Probable EnaEn heterozygotes in two British families.", "content": "An investigation of the serological and biochemical properties of red cells in two unrelated British families revealed the probable presence of examples of the rare genotype EnaEn. In one family the En-modified red cells carried N-like determinants associated with s. In the other family M-like determinants associated with S were found.", "contents": "Probable EnaEn heterozygotes in two British families. An investigation of the serological and biochemical properties of red cells in two unrelated British families revealed the probable presence of examples of the rare genotype EnaEn. In one family the En-modified red cells carried N-like determinants associated with s. In the other family M-like determinants associated with S were found."}
{"id": "PMID:479619", "title": "H-2 linked immune response to murine experimental Schistosoma mansoni infections.", "content": "Mice of two congenic inbred strains C3H/Sn (H-2k) and C3H.B10 (H-2b) were infected with 100 Schistosoma mansoni cercariae. After the infection, the following parameters for the immunological response were studied: worm burden, mortality, antibody titre, spleen index, eosinophilia, delayed type of hypersensitivity and in vitro response to three S. mansoni antigen preparations. No difference in the worm burden and in the in vitro response to the antigen preparations of adult worm antigen, soluble egg antigens and the egg antigen MSA1, was found. The C3H.B10 mice showed a significantly higher mortality, antibody titre and delayed type of hypersensitivity while the C3H/Sn mice showed asignificantly higher spleen index and eosinophilia. This indicates that the H-2 region influences the course of an acute S. mansoni infection, whereas the susceptibility to the infection seems not to be influenced, as is shown by the worm burden.", "contents": "H-2 linked immune response to murine experimental Schistosoma mansoni infections. Mice of two congenic inbred strains C3H/Sn (H-2k) and C3H.B10 (H-2b) were infected with 100 Schistosoma mansoni cercariae. After the infection, the following parameters for the immunological response were studied: worm burden, mortality, antibody titre, spleen index, eosinophilia, delayed type of hypersensitivity and in vitro response to three S. mansoni antigen preparations. No difference in the worm burden and in the in vitro response to the antigen preparations of adult worm antigen, soluble egg antigens and the egg antigen MSA1, was found. The C3H.B10 mice showed a significantly higher mortality, antibody titre and delayed type of hypersensitivity while the C3H/Sn mice showed asignificantly higher spleen index and eosinophilia. This indicates that the H-2 region influences the course of an acute S. mansoni infection, whereas the susceptibility to the infection seems not to be influenced, as is shown by the worm burden."}
{"id": "PMID:479620", "title": "A statistical analysis of human lymphocyte transformation data.", "content": "The lymphocytes from 107 maternal-foetal pairs were examined for their in vitro responsiveness, as determined by the incorporation of tritiated thymidine following stimulation with phytohaemagglutinin (PHA), candida, varicella, mumps, streptokinase-streptodornase (SKSD) and tetanus toxoid. The data were collected and analysed in two sequential groups (forty-seven and sixty) in order to determine whether the results were reproducible. The variable chosen for analysis was the difference (d) between the square roots of the isotope incorporation in the stimulated and control cultures because it gave the most symmetrical distribution of the data. The experimental error in the determination of maternal lymphocyte stimulation was 1.4--8.6% and of the foetal lymphocytes, 1.0--16.6%, depending upon the antigen or mitogen and its concentration. The data in the two sets of patients were statistically the same in forty-eight of the fifty-six analyses (fourteen antigen or mitogen concentrations in autologous and AB plasma for maternal and foetal lymphocytes). The statistical limits of the distribution of responses for stimulation or suppression were set by an analysis of variance taking two standard deviations from the mean as the limits. When these limits were translated into stimulation indices, they varied for each antigen or mitogen and for different concentrations of the same antigen. Thus, a detailed statistical analysis of a large volume of lymphocyte transformation data indicates that the technique is reproducible and offers a reliable method for determing when significant differences from control values are present.", "contents": "A statistical analysis of human lymphocyte transformation data. The lymphocytes from 107 maternal-foetal pairs were examined for their in vitro responsiveness, as determined by the incorporation of tritiated thymidine following stimulation with phytohaemagglutinin (PHA), candida, varicella, mumps, streptokinase-streptodornase (SKSD) and tetanus toxoid. The data were collected and analysed in two sequential groups (forty-seven and sixty) in order to determine whether the results were reproducible. The variable chosen for analysis was the difference (d) between the square roots of the isotope incorporation in the stimulated and control cultures because it gave the most symmetrical distribution of the data. The experimental error in the determination of maternal lymphocyte stimulation was 1.4--8.6% and of the foetal lymphocytes, 1.0--16.6%, depending upon the antigen or mitogen and its concentration. The data in the two sets of patients were statistically the same in forty-eight of the fifty-six analyses (fourteen antigen or mitogen concentrations in autologous and AB plasma for maternal and foetal lymphocytes). The statistical limits of the distribution of responses for stimulation or suppression were set by an analysis of variance taking two standard deviations from the mean as the limits. When these limits were translated into stimulation indices, they varied for each antigen or mitogen and for different concentrations of the same antigen. Thus, a detailed statistical analysis of a large volume of lymphocyte transformation data indicates that the technique is reproducible and offers a reliable method for determing when significant differences from control values are present."}
{"id": "PMID:479621", "title": "Relationship between the responsiveness of maternal and foetal lymphocytes to phytohaemagglutinin and to microbial antigens.", "content": "The response of 105 maternal-foetal lymphocyte pairs to specific and non-specific stimulation were evaluated using a newly defined method of analysis. There were no significant differences in the responses of maternal or foetal lymphocytes to phytohaemogglutinin (PHA) or the various antigens as a function of concentration over the ranges tested. The maternal lymphocytes were stimulated by all of the antigens and responded to PHA three--five times more strongly than to the antigens. The foetal lymphocytes were stimulated by PHA and tetanus toxoid only and were suppressed by streptokinase-streptodornase (SKSD). They responded to stimulation by antigens at a lower level than did the maternal lymphocytes, but they responded at a much higher level to PHA. Unstimulated cultures of foetal lymphocytes incorporated more isotope than did those of maternal lymphocytes in both autologous and AB plasma. The data were cross-classified to determine whether the responses of the foetal lymphocytes varied concordantly with the responses of the maternal lymphocytes in both autologous and AB plasma by the Chi-square test for independence and by rank correlation analysis. There was no significant correlation in either plasma to stimulation with the antigens. Thus, the presence of antigen reactive lymphocytes in the circulation of the mother does not mean that the foetus is sensitized to that antigen. Comparison of the lymphocyte responses in autologous plasma with those in AB plasma provided evidence for the presence of circulating immunoregulatory substances. Autologous maternal plasma suppressed the lymphocyte responses to high concentrations of candida and SKSD and stimulated the response to mumps, varicella and tetanus toxoid. Autologous fetal plasma suppressed the lymphocyte responses to candida, varicella and SKSD and stimulated the response to PHA. The responsiveness of maternal lymphocytes to PHA was less in foetal plasma than in autologous maternal or AB plasma.", "contents": "Relationship between the responsiveness of maternal and foetal lymphocytes to phytohaemagglutinin and to microbial antigens. The response of 105 maternal-foetal lymphocyte pairs to specific and non-specific stimulation were evaluated using a newly defined method of analysis. There were no significant differences in the responses of maternal or foetal lymphocytes to phytohaemogglutinin (PHA) or the various antigens as a function of concentration over the ranges tested. The maternal lymphocytes were stimulated by all of the antigens and responded to PHA three--five times more strongly than to the antigens. The foetal lymphocytes were stimulated by PHA and tetanus toxoid only and were suppressed by streptokinase-streptodornase (SKSD). They responded to stimulation by antigens at a lower level than did the maternal lymphocytes, but they responded at a much higher level to PHA. Unstimulated cultures of foetal lymphocytes incorporated more isotope than did those of maternal lymphocytes in both autologous and AB plasma. The data were cross-classified to determine whether the responses of the foetal lymphocytes varied concordantly with the responses of the maternal lymphocytes in both autologous and AB plasma by the Chi-square test for independence and by rank correlation analysis. There was no significant correlation in either plasma to stimulation with the antigens. Thus, the presence of antigen reactive lymphocytes in the circulation of the mother does not mean that the foetus is sensitized to that antigen. Comparison of the lymphocyte responses in autologous plasma with those in AB plasma provided evidence for the presence of circulating immunoregulatory substances. Autologous maternal plasma suppressed the lymphocyte responses to high concentrations of candida and SKSD and stimulated the response to mumps, varicella and tetanus toxoid. Autologous fetal plasma suppressed the lymphocyte responses to candida, varicella and SKSD and stimulated the response to PHA. The responsiveness of maternal lymphocytes to PHA was less in foetal plasma than in autologous maternal or AB plasma."}
{"id": "PMID:479629", "title": "Accumulation of urinary cancer-related glycoprotein, EDCl, in psoriasis.", "content": "EDCl is a novel glycoprotein, mol wt 27,000, isolated in 1976 from leukemic urine. It inhibits the serine proteases trypsin and chymotrypsin and is antigenically related to interalpha trypsin inhibitor (IATI), mol wt 170,000, a normal plasma antiprotease. Since psoriasis is a non neoplastic hyperproliferative state, we have now measured EDCl by a specific radioimmunoassay (RIA) in plasma and urine of 24 untreated psoriatic patients. EDCl was not detectable in normal urine (less than 1 mg/gm creatinine) or plasma (less than 1 mg/L). 55% of psoriatic urine specimens were positive by RIA, containing 8 to 110 mg/gm creatinine. 75% of plasmas were positive, containing 12 to 32 mg/L. Plasma and urine contents of EDCl were significantly (P less than .05) correlated with severity of clinical disease (% skin involved) but not with age, sex, distribution or type of lesion, family history or arthritis.", "contents": "Accumulation of urinary cancer-related glycoprotein, EDCl, in psoriasis. EDCl is a novel glycoprotein, mol wt 27,000, isolated in 1976 from leukemic urine. It inhibits the serine proteases trypsin and chymotrypsin and is antigenically related to interalpha trypsin inhibitor (IATI), mol wt 170,000, a normal plasma antiprotease. Since psoriasis is a non neoplastic hyperproliferative state, we have now measured EDCl by a specific radioimmunoassay (RIA) in plasma and urine of 24 untreated psoriatic patients. EDCl was not detectable in normal urine (less than 1 mg/gm creatinine) or plasma (less than 1 mg/L). 55% of psoriatic urine specimens were positive by RIA, containing 8 to 110 mg/gm creatinine. 75% of plasmas were positive, containing 12 to 32 mg/L. Plasma and urine contents of EDCl were significantly (P less than .05) correlated with severity of clinical disease (% skin involved) but not with age, sex, distribution or type of lesion, family history or arthritis."}
{"id": "PMID:479630", "title": "DNA-cytophotometry of lymph node imprints from patients with mycosis fungoides.", "content": "To obtain objective criteria for early diagnosis of lymph node involvement in patients with mycosis fundoides, Feulgen DNA-cytophotometry was carried out in lymph node imprints from patients with mycosis fungoides. The lymph nodes of 3 patients with lymph nodes, showing partial or total replacement by atypical lymphoreticular tissue histologically, showed a polyploid and aneuploid DNA distribution. Eleven out of 22 patients with dermatopathic lymphadenopathy both with or without early involvement histologically had an abnormal DNA histogram with hypertetraploid DNA values. Four of these 11 died, 5 had a partial remission in response to therapy and 2 had sustained remission during the follow-up period of 5 yr. The other 11 patients had a normal DNA distribution. Of these 11, one died and 10 achieved sustained remission after therapy. There is a good correlation between the DNA-cytophotometric results and histology of the lymph nodes. On the basis of these results DNA-cytophotometry may be considered an additional and objective aid in the diagnosis of lymph node involvement in mycosis fungoides.", "contents": "DNA-cytophotometry of lymph node imprints from patients with mycosis fungoides. To obtain objective criteria for early diagnosis of lymph node involvement in patients with mycosis fundoides, Feulgen DNA-cytophotometry was carried out in lymph node imprints from patients with mycosis fungoides. The lymph nodes of 3 patients with lymph nodes, showing partial or total replacement by atypical lymphoreticular tissue histologically, showed a polyploid and aneuploid DNA distribution. Eleven out of 22 patients with dermatopathic lymphadenopathy both with or without early involvement histologically had an abnormal DNA histogram with hypertetraploid DNA values. Four of these 11 died, 5 had a partial remission in response to therapy and 2 had sustained remission during the follow-up period of 5 yr. The other 11 patients had a normal DNA distribution. Of these 11, one died and 10 achieved sustained remission after therapy. There is a good correlation between the DNA-cytophotometric results and histology of the lymph nodes. On the basis of these results DNA-cytophotometry may be considered an additional and objective aid in the diagnosis of lymph node involvement in mycosis fungoides."}
{"id": "PMID:479631", "title": "Comparison of eumelanogenesis and pheomelanogenesis in retinal and follicular melanocytes; role of vesiculo-globular bodies in melanosome differentiation.", "content": "Vesiculo-globular bodies, 40 nm in diameter, are present in melanosomes. The mode of their involvement in melanosomal differentiation was studied by ultrastructural comparison of eu- and pheomelanogenesis occurring in retinal and follicular melanocytes. We found that the number and distribution of these bodies differ significantly with types of melanogenesis and tissues. They were not affected by physical stimuli nor by embryonic origin of melanocytes. The earliest form of melanosomes is identical in eu- and pheomelanogenesis. The vesiculo-globular bodies are involved in organization of melanosomal constituents. In eumelanogenesis, they are more numerous in feather than in retina and hair. They are least numerous in white hair and pink eyes where melanization is blocked. During melanosomal development, they become associated with melanosomal inner lamellae and their outer surface becomes melanized, but their core is hardly melanized, thus leaving small vesicular structures. In pheomelanogenesis, their number is almost equal in feather and hair. Lamellae are not formed, but these bodies fuse with each other to form an amorphous matrix on complete differentiation of melanosomes.", "contents": "Comparison of eumelanogenesis and pheomelanogenesis in retinal and follicular melanocytes; role of vesiculo-globular bodies in melanosome differentiation. Vesiculo-globular bodies, 40 nm in diameter, are present in melanosomes. The mode of their involvement in melanosomal differentiation was studied by ultrastructural comparison of eu- and pheomelanogenesis occurring in retinal and follicular melanocytes. We found that the number and distribution of these bodies differ significantly with types of melanogenesis and tissues. They were not affected by physical stimuli nor by embryonic origin of melanocytes. The earliest form of melanosomes is identical in eu- and pheomelanogenesis. The vesiculo-globular bodies are involved in organization of melanosomal constituents. In eumelanogenesis, they are more numerous in feather than in retina and hair. They are least numerous in white hair and pink eyes where melanization is blocked. During melanosomal development, they become associated with melanosomal inner lamellae and their outer surface becomes melanized, but their core is hardly melanized, thus leaving small vesicular structures. In pheomelanogenesis, their number is almost equal in feather and hair. Lamellae are not formed, but these bodies fuse with each other to form an amorphous matrix on complete differentiation of melanosomes."}
{"id": "PMID:479632", "title": "Multiple forms of epidermal alpha-fucosidase.", "content": "Two forms of alpha-fucosidase from newborn rat epidermis were separated by gel filtration on Sephadex G-150. These enzymes termed, fucosidase I which was eluted in the void volume and fucosidase II (molecular weight approximately 50,000). Both enzymes had pH optima for 4-methylumbelliferyl-alpha-L-fucoside hydrolysis between 5.5-6.5. Km values for fucosidase I and II with the same substrate were 3.7 X 10(-5) and 5.4 X 10(-5) M, respectively. Three different forms of new born rat epidermal alpha-fucosidase were separated by isoelectric focusing. Evidence is present which indicates that the electrophoretic heterogeneity of alpha-fucosidase is due in part to the binding of sialic acid to the primary gene product.", "contents": "Multiple forms of epidermal alpha-fucosidase. Two forms of alpha-fucosidase from newborn rat epidermis were separated by gel filtration on Sephadex G-150. These enzymes termed, fucosidase I which was eluted in the void volume and fucosidase II (molecular weight approximately 50,000). Both enzymes had pH optima for 4-methylumbelliferyl-alpha-L-fucoside hydrolysis between 5.5-6.5. Km values for fucosidase I and II with the same substrate were 3.7 X 10(-5) and 5.4 X 10(-5) M, respectively. Three different forms of new born rat epidermal alpha-fucosidase were separated by isoelectric focusing. Evidence is present which indicates that the electrophoretic heterogeneity of alpha-fucosidase is due in part to the binding of sialic acid to the primary gene product."}
{"id": "PMID:479633", "title": "Kinin-forming enzyme in human skin: the purification and characterization of a kinin-forming enzyme.", "content": "A kinin-forming-enzyme in human skin extract was further purified by successive column chromatography on DEAE-cellulose, Hydroxylapatite-cellulose and Sepharose-4B. By these procedures, 2.7 mg of purified enzyme was obtained from 10 gm of original skin. The purified material was homogeneous as ascertained by cellulose acetate membrane electrophoresis, sodium dodecyl sulfate polyacrylamide gel disc electrophoresis and ultracentrifugation. It had an S20,w value of 4.3 and an apparent molecular weight of 104,000 as measured by gel filtration on Sephadex G-200. The purified enzyme was comparatively heat-stable, but was unstable below pH values of 5 and above pH 9. It possessed arginine or lysine esterolytic activity, but not tyrosine or tryptophane esterolytic activity and denatured proteolytic activity. This enzyme was not affected by metal ion, cystein, glutathion or rho-chloromercuribenzoate, but was strongly inhibited by alpha-N-rho-tosyl-L-lysine chloromethyl ketone or soybean-trypsin inhibitor. It was also inhibited by alpha 1-antitrypsin, but not by alpha 2-macroglobulin. This enzyme was confirmed to be immunologically distinct from human plasma, urinary or pancreas kallikrein.", "contents": "Kinin-forming enzyme in human skin: the purification and characterization of a kinin-forming enzyme. A kinin-forming-enzyme in human skin extract was further purified by successive column chromatography on DEAE-cellulose, Hydroxylapatite-cellulose and Sepharose-4B. By these procedures, 2.7 mg of purified enzyme was obtained from 10 gm of original skin. The purified material was homogeneous as ascertained by cellulose acetate membrane electrophoresis, sodium dodecyl sulfate polyacrylamide gel disc electrophoresis and ultracentrifugation. It had an S20,w value of 4.3 and an apparent molecular weight of 104,000 as measured by gel filtration on Sephadex G-200. The purified enzyme was comparatively heat-stable, but was unstable below pH values of 5 and above pH 9. It possessed arginine or lysine esterolytic activity, but not tyrosine or tryptophane esterolytic activity and denatured proteolytic activity. This enzyme was not affected by metal ion, cystein, glutathion or rho-chloromercuribenzoate, but was strongly inhibited by alpha-N-rho-tosyl-L-lysine chloromethyl ketone or soybean-trypsin inhibitor. It was also inhibited by alpha 1-antitrypsin, but not by alpha 2-macroglobulin. This enzyme was confirmed to be immunologically distinct from human plasma, urinary or pancreas kallikrein."}
{"id": "PMID:479634", "title": "The determination of lipids and proteins in suction blister fluid.", "content": "The concentration of 5 different proteins in suction blister fluid and serum was determined by immunotechniques. These proteins, varying in size and molecular weight (6,600-2,300,000) were insulin, albumin, high density lipoprotein determined as apoprotein A-I, alpha 2-macroglobulin and low density lipoprotein measured as apoprotein B. The difference in the blister fluid/serum concentration ratio of the proteins was dependent on the molecular weight and followed mainly the law of diffusion. Moreover, the amounts of insulin, albumin and apoproteins A-I and B in suction blister fluid were the same as those reported in peripheral lymph. The results indicate that the sieve function of the capillary basement membrane remains intact during the formation of the suction blisters. Suction blister fluid might therefore be regarded as representative of interstitial fluid. The concentrations of 4 different lipids (cholesterol, cholesterolesters, triglycerides and phospholipids) were also determined and their blister fluid/serum concentration ratio proved to have a fairly constant value of 0.25.", "contents": "The determination of lipids and proteins in suction blister fluid. The concentration of 5 different proteins in suction blister fluid and serum was determined by immunotechniques. These proteins, varying in size and molecular weight (6,600-2,300,000) were insulin, albumin, high density lipoprotein determined as apoprotein A-I, alpha 2-macroglobulin and low density lipoprotein measured as apoprotein B. The difference in the blister fluid/serum concentration ratio of the proteins was dependent on the molecular weight and followed mainly the law of diffusion. Moreover, the amounts of insulin, albumin and apoproteins A-I and B in suction blister fluid were the same as those reported in peripheral lymph. The results indicate that the sieve function of the capillary basement membrane remains intact during the formation of the suction blisters. Suction blister fluid might therefore be regarded as representative of interstitial fluid. The concentrations of 4 different lipids (cholesterol, cholesterolesters, triglycerides and phospholipids) were also determined and their blister fluid/serum concentration ratio proved to have a fairly constant value of 0.25."}
{"id": "PMID:479635", "title": "Local and systemic effects on the epidermal melanocyte population in UV-irradiated mouse skin.", "content": "Local and systemic effects of repeated UVB irradiation on the epidermal melanocytes have been studied in the C57Bl mice. A daily dose of 0.1 joule/cm2 for 10 days induced a 4-fold increase in the epidermal melanocyte population of the irradiated right ear. During the first weeks after the irradiation period, there was a gradual increase in the number of melanocytes also in the shielded left ear, up to about 3 times the age control values. Thereafter, the population density slowly decreased in both ears, but it remained well above original values as late as 20 weeks after the irradiation. Thus, a short UVB irradiation period induces a long-lasting increase in the number of epidermal melanocytes in irradiated skin areas, as well as in covered skin regions. It is suggested that the population increase in the shielded ear is initiated by one or more systemic factors originating from the UVB irradiated skin. Such factors may be involved in the regulation of a balanced melanocyte population over the entrie body surface.", "contents": "Local and systemic effects on the epidermal melanocyte population in UV-irradiated mouse skin. Local and systemic effects of repeated UVB irradiation on the epidermal melanocytes have been studied in the C57Bl mice. A daily dose of 0.1 joule/cm2 for 10 days induced a 4-fold increase in the epidermal melanocyte population of the irradiated right ear. During the first weeks after the irradiation period, there was a gradual increase in the number of melanocytes also in the shielded left ear, up to about 3 times the age control values. Thereafter, the population density slowly decreased in both ears, but it remained well above original values as late as 20 weeks after the irradiation. Thus, a short UVB irradiation period induces a long-lasting increase in the number of epidermal melanocytes in irradiated skin areas, as well as in covered skin regions. It is suggested that the population increase in the shielded ear is initiated by one or more systemic factors originating from the UVB irradiated skin. Such factors may be involved in the regulation of a balanced melanocyte population over the entrie body surface."}
{"id": "PMID:479636", "title": "Immunization of pregnant women with influenza A/New Jersey/76 virus vaccine: reactogenicity and immunogenicity in mother and infant.", "content": "The safety and immunogenicity of inactivated influenza virus vaccines in pregnant women have not been adequately investigated. In this study, 56 women received inactivated influenza A/New Jersey/76 virus vaccine during the second and third trimesters of pregnancy. No significant immediate reactions or increased fetal complications were associated with administration of the vaccine. The antibody response of the pregnant women to the vaccine was similar to that of nonpregnant adults. Forty mother-infant pairs were available for antibody surveillance. At delivery, reciprocal antibody titers of greater than or equal to 20 were present in 11 (42%) newborn (cord) sera and 15 (58%) maternal sera. Three months later, sera from only three infants (12%) contained this level of antibody. At six months, the serum of only one infant contained this level of antibody. At six months, the serum of only one infant contained detectable antibodies. Levels of passively transferred antibodies from prior maternal infection with influenza A/Victoria/75 virus also declined rapidly following birth. It is possible that immunization of pregnant women can provide sufficient protection of the newborn infants by transfer of antibodies through the placenta if (1) a more potent influenza vaccine, possibly used with booster dosing, is administered, and (2) the women deliver just prior to or during the influenza season.", "contents": "Immunization of pregnant women with influenza A/New Jersey/76 virus vaccine: reactogenicity and immunogenicity in mother and infant. The safety and immunogenicity of inactivated influenza virus vaccines in pregnant women have not been adequately investigated. In this study, 56 women received inactivated influenza A/New Jersey/76 virus vaccine during the second and third trimesters of pregnancy. No significant immediate reactions or increased fetal complications were associated with administration of the vaccine. The antibody response of the pregnant women to the vaccine was similar to that of nonpregnant adults. Forty mother-infant pairs were available for antibody surveillance. At delivery, reciprocal antibody titers of greater than or equal to 20 were present in 11 (42%) newborn (cord) sera and 15 (58%) maternal sera. Three months later, sera from only three infants (12%) contained this level of antibody. At six months, the serum of only one infant contained this level of antibody. At six months, the serum of only one infant contained detectable antibodies. Levels of passively transferred antibodies from prior maternal infection with influenza A/Victoria/75 virus also declined rapidly following birth. It is possible that immunization of pregnant women can provide sufficient protection of the newborn infants by transfer of antibodies through the placenta if (1) a more potent influenza vaccine, possibly used with booster dosing, is administered, and (2) the women deliver just prior to or during the influenza season."}
{"id": "PMID:479637", "title": "HLA-linked genes and leprosy: a family study in Karigiri, South India.", "content": "The evidence for a genetic determination of susceptibility to leprosy is reviewed. To test the hypothesis that an HLA (histocompatibility leukocyte antigen)-linked gene is associated with such susceptibility, the association between the distribution of leprosy within a family and the segregation of HLA haplotypes was investigated among 72 families who lived in Karigiri, Tamil Nadu State, South India. A statistically significant association was found for families in which siblings had tuberculoid leprosy and in which neither parent had leprosy. The findings from the data of this study agree with those of two previous studies carried out among smaller populations is Surinam and Wardha, Maharashtra State, India. Such an agreement suggests that a genetic determinant which is linked to the major HLA locus on chromosome 6 and which is probably recessive affects susceptibility to tuberculoid leprosy in humans.", "contents": "HLA-linked genes and leprosy: a family study in Karigiri, South India. The evidence for a genetic determination of susceptibility to leprosy is reviewed. To test the hypothesis that an HLA (histocompatibility leukocyte antigen)-linked gene is associated with such susceptibility, the association between the distribution of leprosy within a family and the segregation of HLA haplotypes was investigated among 72 families who lived in Karigiri, Tamil Nadu State, South India. A statistically significant association was found for families in which siblings had tuberculoid leprosy and in which neither parent had leprosy. The findings from the data of this study agree with those of two previous studies carried out among smaller populations is Surinam and Wardha, Maharashtra State, India. Such an agreement suggests that a genetic determinant which is linked to the major HLA locus on chromosome 6 and which is probably recessive affects susceptibility to tuberculoid leprosy in humans."}
{"id": "PMID:479638", "title": "Immune responses of humans to a human diploid cell strain of rabies virus vaccine: lymphocyte transformation, production of virus-neutralizing antibody, and induction of interferon.", "content": "Lymphocyte transformation, production of neutralizing antibody, and interferon activity of 20 healthy volunteers in response to a human diploid cell strain (HDCS) of rabies virus vaccine were studied. Ten vaccines received 1.0 ml of HDCS vaccine on days 0, 3, 7, and 14, and five of these volunteers received, in addition, 20 international units of human rabies immune globulin/kg of body weight on day 0. All 10 volunteers developed high titers of neutralizing antibody, and eight of the 10 had lymphocytes that were immunologically stimulated by HDCS rabies virus antigen. The interferon responses of eight vaccines to 1.0 ml of HDCS vaccine given intramuscularly on days 0 and 28 and of two vaccines to 1.0 ml of vaccine given intradermally in eight body sites on the same days were measured; low levels of interferon-like activity were found in eight of nine volunteers after the first vaccination, and no activity was found upon revaccination. The high titers of neutralizing antibody that developed did not correlate with lymphocyte stimulation or interferon-like activity, but it is true that all 20 volunteers did develop high titers of neutralizing antibody after 1.0 ml of HDCS vaccine was given intradermally.", "contents": "Immune responses of humans to a human diploid cell strain of rabies virus vaccine: lymphocyte transformation, production of virus-neutralizing antibody, and induction of interferon. Lymphocyte transformation, production of neutralizing antibody, and interferon activity of 20 healthy volunteers in response to a human diploid cell strain (HDCS) of rabies virus vaccine were studied. Ten vaccines received 1.0 ml of HDCS vaccine on days 0, 3, 7, and 14, and five of these volunteers received, in addition, 20 international units of human rabies immune globulin/kg of body weight on day 0. All 10 volunteers developed high titers of neutralizing antibody, and eight of the 10 had lymphocytes that were immunologically stimulated by HDCS rabies virus antigen. The interferon responses of eight vaccines to 1.0 ml of HDCS vaccine given intramuscularly on days 0 and 28 and of two vaccines to 1.0 ml of vaccine given intradermally in eight body sites on the same days were measured; low levels of interferon-like activity were found in eight of nine volunteers after the first vaccination, and no activity was found upon revaccination. The high titers of neutralizing antibody that developed did not correlate with lymphocyte stimulation or interferon-like activity, but it is true that all 20 volunteers did develop high titers of neutralizing antibody after 1.0 ml of HDCS vaccine was given intradermally."}
{"id": "PMID:479639", "title": "Selection of small-colony variants of Enterobacteriaceae by in vitro exposure to aminoglycosides: pathogenicity for experimental animals.", "content": "Small-colony variants of gram-negative genera of Enterobacteriaceae were selected by in vitro exposure to gentamicin. These variants were shown to have decreased susceptibility in vitro to aminoglycosides. They were lethal for mice following intraperitoneal injection, with the LD50 (50% lethal dose) being the same as, or slightly less than, that for the parent organism. Variants of strain no. 2401 of Proteus mirabilis caused urinary tract infection in mice after implantation into their bladders. Although the variants grew somewhat less rapidly than did parent organisms, both parent and variant colonies alkalinized urine at the same rate. Electron microscopic study showed no differences between colonies of parents and variants. These studies indicated that small-colony variants of Enterobacteriaceae are pathogenic for experimental animals. Further, they may cause disease in humans and should not necessarily be regarded as only laboratory curiosities.", "contents": "Selection of small-colony variants of Enterobacteriaceae by in vitro exposure to aminoglycosides: pathogenicity for experimental animals. Small-colony variants of gram-negative genera of Enterobacteriaceae were selected by in vitro exposure to gentamicin. These variants were shown to have decreased susceptibility in vitro to aminoglycosides. They were lethal for mice following intraperitoneal injection, with the LD50 (50% lethal dose) being the same as, or slightly less than, that for the parent organism. Variants of strain no. 2401 of Proteus mirabilis caused urinary tract infection in mice after implantation into their bladders. Although the variants grew somewhat less rapidly than did parent organisms, both parent and variant colonies alkalinized urine at the same rate. Electron microscopic study showed no differences between colonies of parents and variants. These studies indicated that small-colony variants of Enterobacteriaceae are pathogenic for experimental animals. Further, they may cause disease in humans and should not necessarily be regarded as only laboratory curiosities."}
{"id": "PMID:479640", "title": "Ultrastructural observations on giardiasis in a mouse model. II. Endosymbiosis and organelle distribution in Giardia muris and Giardia lamblia.", "content": "Ultrastructural observations of Giardia muris in a mouse model revealed endosymbiotic microbes not previously reported in Giardia. Endosymbionts 240--360 nm wide, 600--1,400 nm long, and with an internal structure similar to that of bacilli were not seen entering Giardia but were found and appeared to divide within Giardia. No evidence was found of digestion of the endosymbionts by the giardia host in either the trophozoite or the cyst form. Endosymbionts were concentrated centrally around the nuclear area and were uncommon in peripheral feeding regions. The same cellular organelles seen in G. muris were found in Giardia lamblia from human jejunal biopsy material, but no endosymbionts were identified in G. lamblia trophozoites from the seven patients examined. Endosymbionts within Giardia may be found to alter trophozoite pathogenicity, metabolism, range of infectivity, antigenic surface characteristics, and host specificity, as they do in other protozoa.", "contents": "Ultrastructural observations on giardiasis in a mouse model. II. Endosymbiosis and organelle distribution in Giardia muris and Giardia lamblia. Ultrastructural observations of Giardia muris in a mouse model revealed endosymbiotic microbes not previously reported in Giardia. Endosymbionts 240--360 nm wide, 600--1,400 nm long, and with an internal structure similar to that of bacilli were not seen entering Giardia but were found and appeared to divide within Giardia. No evidence was found of digestion of the endosymbionts by the giardia host in either the trophozoite or the cyst form. Endosymbionts were concentrated centrally around the nuclear area and were uncommon in peripheral feeding regions. The same cellular organelles seen in G. muris were found in Giardia lamblia from human jejunal biopsy material, but no endosymbionts were identified in G. lamblia trophozoites from the seven patients examined. Endosymbionts within Giardia may be found to alter trophozoite pathogenicity, metabolism, range of infectivity, antigenic surface characteristics, and host specificity, as they do in other protozoa."}
{"id": "PMID:479641", "title": "Comparison of responses to influenza A/New Jersey/76-A/Victoria/75 virus vaccine administered intradermally or subcutaneously to adults with chronic respiratory disease.", "content": "Serum HAI (hemagglutination inhibition) antibody responses were compared in two groups of 70 age-matched patients (age range, 17 to 82 years) who were vaccinated with bivalent influenza A/New Jersey/76-A/Victoria/75 whole-virus vaccine. The group that was vaccinated intradermally received 40 chick cell-agglutinating units of each viral antigen in 0.1 ml, and the group that was vaccinated subcutaneously received 200 chick cell-agglutinating units of each antigen in 0.5 ml. The serum HAI antibody response to A/New Jersey/76 antigen was significantly higher in the group that was vaccinated subcutaneously; this difference was particularly evident in patients less than or equal to 50 years old. The serologic response to A/Victoria/75 antigen did not differ significantly between the two groups. Levels of antibody before vaccination indicated previous widespread exposure of patients to influenza A/Victoria/75 virus, but not to influenza A/New Jersey/76 virus. Such differences in prior immunologic experience with a particular strain of influenza virus probably determine whether the intradermal route of vaccination is as effective as, or inferior to, the subcutaneous route.", "contents": "Comparison of responses to influenza A/New Jersey/76-A/Victoria/75 virus vaccine administered intradermally or subcutaneously to adults with chronic respiratory disease. Serum HAI (hemagglutination inhibition) antibody responses were compared in two groups of 70 age-matched patients (age range, 17 to 82 years) who were vaccinated with bivalent influenza A/New Jersey/76-A/Victoria/75 whole-virus vaccine. The group that was vaccinated intradermally received 40 chick cell-agglutinating units of each viral antigen in 0.1 ml, and the group that was vaccinated subcutaneously received 200 chick cell-agglutinating units of each antigen in 0.5 ml. The serum HAI antibody response to A/New Jersey/76 antigen was significantly higher in the group that was vaccinated subcutaneously; this difference was particularly evident in patients less than or equal to 50 years old. The serologic response to A/Victoria/75 antigen did not differ significantly between the two groups. Levels of antibody before vaccination indicated previous widespread exposure of patients to influenza A/Victoria/75 virus, but not to influenza A/New Jersey/76 virus. Such differences in prior immunologic experience with a particular strain of influenza virus probably determine whether the intradermal route of vaccination is as effective as, or inferior to, the subcutaneous route."}
{"id": "PMID:479663", "title": "Peritoneal clearances.", "content": "Intermittent peritoneal dialysis may provide borderline adequate dialysis in terms of clearance of small solutes. A number of disease processes could decrease clearances. Drug manipulations and other techniques to increase clearances primarily affect larger solutes. Since some small-molecular-weight solutes may be uremic toxins until proven otherwise, small solute clearance decreases during chronic intermittent peritoneal dialysis may require consideration of even more hours of intermittent peritoneal dialysis or initiation of CAPD.", "contents": "Peritoneal clearances. Intermittent peritoneal dialysis may provide borderline adequate dialysis in terms of clearance of small solutes. A number of disease processes could decrease clearances. Drug manipulations and other techniques to increase clearances primarily affect larger solutes. Since some small-molecular-weight solutes may be uremic toxins until proven otherwise, small solute clearance decreases during chronic intermittent peritoneal dialysis may require consideration of even more hours of intermittent peritoneal dialysis or initiation of CAPD."}
{"id": "PMID:479664", "title": "Measurement of peritoneal clearances in self-dialysis patients.", "content": "Twelve chronic peritoneal dialysis patients were studied on 20 occasions to measure peritoneal clearances of creatinine, urea, uric acid, and potassium. Two clearance techniques were employed, the classical type using timed collections and a midpoint blood specimen and an experimental type using calculated dialysate flow and predialysis peritoneal fluid solute concentrations. Comparison of the two techniques demonstrated a close correlation between simultaneously measured clearances and also for repeat measurements in the same patient. The new technique is simple and reproducible, does not require blood sampling, and can be performed in the home by patients treated by self-dialysis using automated peritoneal dialysis equipment. The new technique will allow more frequent monitoring of peritoneal permeance in self-dialysis patients, and necessary adjustments in treatment time can be prescribed without requiring hospitalization.", "contents": "Measurement of peritoneal clearances in self-dialysis patients. Twelve chronic peritoneal dialysis patients were studied on 20 occasions to measure peritoneal clearances of creatinine, urea, uric acid, and potassium. Two clearance techniques were employed, the classical type using timed collections and a midpoint blood specimen and an experimental type using calculated dialysate flow and predialysis peritoneal fluid solute concentrations. Comparison of the two techniques demonstrated a close correlation between simultaneously measured clearances and also for repeat measurements in the same patient. The new technique is simple and reproducible, does not require blood sampling, and can be performed in the home by patients treated by self-dialysis using automated peritoneal dialysis equipment. The new technique will allow more frequent monitoring of peritoneal permeance in self-dialysis patients, and necessary adjustments in treatment time can be prescribed without requiring hospitalization."}
{"id": "PMID:479665", "title": "Binding of IgG and complement protein by monosodium urate monohydrate and other crystals.", "content": "IgG adsorbed to negatively charged crystals of monosodium urate monohydrate was restricted to cationic species of immunoglobulin. Similar binding of smaller amounts of IgG was observed with crystals other than monosodium urate monohydrate. Heated monosodium urate monohydrate and amorphous sodium urate adsorbed greater amounts of IgG and other proteins. Complement activation as measured by electrophoretic conversion of C3, was not related to the amount of IgG adsorbed by the crystals studied. C1q was also adsorbed to some degree by all crystals studied but did not correlate with complement activation.", "contents": "Binding of IgG and complement protein by monosodium urate monohydrate and other crystals. IgG adsorbed to negatively charged crystals of monosodium urate monohydrate was restricted to cationic species of immunoglobulin. Similar binding of smaller amounts of IgG was observed with crystals other than monosodium urate monohydrate. Heated monosodium urate monohydrate and amorphous sodium urate adsorbed greater amounts of IgG and other proteins. Complement activation as measured by electrophoretic conversion of C3, was not related to the amount of IgG adsorbed by the crystals studied. C1q was also adsorbed to some degree by all crystals studied but did not correlate with complement activation."}
{"id": "PMID:479668", "title": "Removal of bile acids and bilirubin by plasmaperfusion of U.S.P. charcoal-coated glass beads.", "content": "Removal of protein-bound cholephilic substances such as BA by sorbent perfusion might improve the quality of life of patients with severe inoperable cholestasis where the accumulation of BA is thought to be responsible for the often distressing pruritus. Since hemoperfusion is associated with hematological side effects, plasmaperfusion which, in addition, allows the use of novel, possibly more efficient sorbents, might be preferable, provided a substance with a high affinity for these protein-bound anions could be found. A number of sorbents suitable for plasma-perfusion were therefore tested in vitro as to their BA-adsorbing capacity. U.S.P. charcoal coated onto glass beads in order to facilitate perfusion removed BA from human plasma far better than conventional charcoal suitable for hemoperfusion, charged and uncharged resins, and immobilized albumin. The extraction of BA by the coated glass beads exceeded 90% up to a load of 0.18 mumol BA per milliliter of sorbent, and the BA-adsorbing capacity was 20.8 mumol/ml. In addition to BA, the U.S.P. charcoal columns almost quantitatively removed uric acid, creatinine, and corticosteroids and retained substantial amounts of BR and thyroxine, whereas electrolytes, clotting factors, and plasmaproteins were adsorbed only initially.", "contents": "Removal of bile acids and bilirubin by plasmaperfusion of U.S.P. charcoal-coated glass beads. Removal of protein-bound cholephilic substances such as BA by sorbent perfusion might improve the quality of life of patients with severe inoperable cholestasis where the accumulation of BA is thought to be responsible for the often distressing pruritus. Since hemoperfusion is associated with hematological side effects, plasmaperfusion which, in addition, allows the use of novel, possibly more efficient sorbents, might be preferable, provided a substance with a high affinity for these protein-bound anions could be found. A number of sorbents suitable for plasma-perfusion were therefore tested in vitro as to their BA-adsorbing capacity. U.S.P. charcoal coated onto glass beads in order to facilitate perfusion removed BA from human plasma far better than conventional charcoal suitable for hemoperfusion, charged and uncharged resins, and immobilized albumin. The extraction of BA by the coated glass beads exceeded 90% up to a load of 0.18 mumol BA per milliliter of sorbent, and the BA-adsorbing capacity was 20.8 mumol/ml. In addition to BA, the U.S.P. charcoal columns almost quantitatively removed uric acid, creatinine, and corticosteroids and retained substantial amounts of BR and thyroxine, whereas electrolytes, clotting factors, and plasmaproteins were adsorbed only initially."}
{"id": "PMID:479669", "title": "Micellar properties of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oyl taurine and relationship to in vitro red cell disruption.", "content": "Patients with a metabolic block in the conversion of THCA to cholic acid develop cirrhosis and hemolysis. Tauro-THCA has been shown to distort hepatic architecture and cause hemolysis in bile-fistula rats. In this study, the critical micellular concentration of tauro-THCA was found to be one fourth of that measured for the primary human bile salt, taurocholate. In short-term incubations with intact red cells, tauro-THCA was more effective than taurocholate in removing red cell membrane lipid, inducing morphological red cell sphering, and decreasing functional cellular membrane surface area. These detergent biological membrane effects were most apparent at a concentration above the critical micellar concentration, with the membrane toxicity of the two bile salts roughly paralleling their differences in critical micellar concentration. The lower critical micellar concentration, greater hydrophobicity, and enhanced surface-active properties of tauro-THCA are speculated on as possible factors contributing to the bile salt's toxicity in vivo.", "contents": "Micellar properties of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oyl taurine and relationship to in vitro red cell disruption. Patients with a metabolic block in the conversion of THCA to cholic acid develop cirrhosis and hemolysis. Tauro-THCA has been shown to distort hepatic architecture and cause hemolysis in bile-fistula rats. In this study, the critical micellular concentration of tauro-THCA was found to be one fourth of that measured for the primary human bile salt, taurocholate. In short-term incubations with intact red cells, tauro-THCA was more effective than taurocholate in removing red cell membrane lipid, inducing morphological red cell sphering, and decreasing functional cellular membrane surface area. These detergent biological membrane effects were most apparent at a concentration above the critical micellar concentration, with the membrane toxicity of the two bile salts roughly paralleling their differences in critical micellar concentration. The lower critical micellar concentration, greater hydrophobicity, and enhanced surface-active properties of tauro-THCA are speculated on as possible factors contributing to the bile salt's toxicity in vivo."}
{"id": "PMID:479670", "title": "Serum amyloid P-component levels in amyloidosis, connective tissue diseases, infection, and malignancy as compared to normal serum.", "content": "A highly sensitive radioimmunoassay was developed to measure the serum levels of SAP. Sera from 50 normal individuals ranging in age from 4 to 89 years had a mean level of 7.47 mg/dl and showed no variations with age. Sera from persons with various clinical types of amyloidosis, connective tissue diseases, and bacterial pneumonia did not differ significantly from normal values. A significant difference was noted in the sera from persons with malignancy, where a mean value of 10.79 mg/dl was determined. Although a number of similarities exist between SAP and CRP, SAP does not share the property of being an acute-phase reactant.", "contents": "Serum amyloid P-component levels in amyloidosis, connective tissue diseases, infection, and malignancy as compared to normal serum. A highly sensitive radioimmunoassay was developed to measure the serum levels of SAP. Sera from 50 normal individuals ranging in age from 4 to 89 years had a mean level of 7.47 mg/dl and showed no variations with age. Sera from persons with various clinical types of amyloidosis, connective tissue diseases, and bacterial pneumonia did not differ significantly from normal values. A significant difference was noted in the sera from persons with malignancy, where a mean value of 10.79 mg/dl was determined. Although a number of similarities exist between SAP and CRP, SAP does not share the property of being an acute-phase reactant."}
{"id": "PMID:479671", "title": "A solid-phase radioimmunoassay for bound anti-platelet antibody: studies on 45 patients with autoimmune platelet disorders.", "content": "A highly sensitive, solid-phase radioimmunoassay has been developed for detecting platelet-bound immunoglobulin, which employs 125I-staphylococcal protein A. The assay detects platelet IgG at the picogram level, which is 10- to 50-fold more sensitive than currently available procedures. It is relatively simple and can be performed on frozen extracts from as little as 2 X 10(5) platelets. Washed platelets are frozen, thawed, sonicated, and centrifuged at 20,000 X g. The supernatant, which contains 7S IgG, is applied in serial dilution to the wells of a plastic microliter plate capable of adsorbing protein. Commercial rabbit anti-human IgG is added and then \"sandwiched\" to 125I-staphylococcal protein A. The wells are removed from the plate and assayed for radioactivity. Thirty-two of 35 thrombocytopenic patients (92%) with ATP had platelet IgG values greater than those of controls by 2 S.D. and averaged 150 +/- 145 (S.D.) ng/10(6) platelets; 11 healthy controls, 11.4 +/- 7.4 ng; five \"thrombocytopenic\" controls, 9.7 +/- 13 ng. The platelet count (X) correlated inversely with IgG/platelet (Y) according to the equation: Log Y = -0.66 log X + 4.8; r = -0.71, p less than 0.001. Elevated platelet IgG levels were also found in 5 ATP patients in \"apparent remission\"; two of four had evidence for compensated thrombocytolysis (increased megathrombocytes). Elevated platelet IgG levels were also found in six patients with thrombocytopenia secondary to lymphoproliferative disorders.", "contents": "A solid-phase radioimmunoassay for bound anti-platelet antibody: studies on 45 patients with autoimmune platelet disorders. A highly sensitive, solid-phase radioimmunoassay has been developed for detecting platelet-bound immunoglobulin, which employs 125I-staphylococcal protein A. The assay detects platelet IgG at the picogram level, which is 10- to 50-fold more sensitive than currently available procedures. It is relatively simple and can be performed on frozen extracts from as little as 2 X 10(5) platelets. Washed platelets are frozen, thawed, sonicated, and centrifuged at 20,000 X g. The supernatant, which contains 7S IgG, is applied in serial dilution to the wells of a plastic microliter plate capable of adsorbing protein. Commercial rabbit anti-human IgG is added and then \"sandwiched\" to 125I-staphylococcal protein A. The wells are removed from the plate and assayed for radioactivity. Thirty-two of 35 thrombocytopenic patients (92%) with ATP had platelet IgG values greater than those of controls by 2 S.D. and averaged 150 +/- 145 (S.D.) ng/10(6) platelets; 11 healthy controls, 11.4 +/- 7.4 ng; five \"thrombocytopenic\" controls, 9.7 +/- 13 ng. The platelet count (X) correlated inversely with IgG/platelet (Y) according to the equation: Log Y = -0.66 log X + 4.8; r = -0.71, p less than 0.001. Elevated platelet IgG levels were also found in 5 ATP patients in \"apparent remission\"; two of four had evidence for compensated thrombocytolysis (increased megathrombocytes). Elevated platelet IgG levels were also found in six patients with thrombocytopenia secondary to lymphoproliferative disorders."}
{"id": "PMID:479672", "title": "Effect of erythrocyte destruction on the pulmonary excretion rate of carbon monoxide in adult male Wistar rats.", "content": "One of the major contributory factors to hyperbilirubinemia in the human infant is bilirubin production. Determination of the pulmonary excretion rate of carbon monoxide is used to measure indirectly the rate of bilirubin production by estimating the endogenous production of CO, which is formed in equimolar amounts with bilirubin in the catabolism of heme. There has been no study to confirm that complete recovery of CO produced in vivo from the catabolism of known amounts of heme occurs via pulmonary excretion. We report here quantitative determinations of the pulmonary excretion rate of CO in five adult male Wistar rats after injection of known amounts of heme in the form of red blood cells \"damaged\" by incubation in a solution containing a sulfhydryl inhibitor, NEM. The mean recovery of \"extra\" CO above baseline production represented as a molar ratio of extra CO to heme was 0.98 +/- 0.02 (S.E.). Control studies showed no extra CO production after starvation, injection of NEM in an amount comparable to that used in the experimental animals, or injection of undamaged red blood cells.", "contents": "Effect of erythrocyte destruction on the pulmonary excretion rate of carbon monoxide in adult male Wistar rats. One of the major contributory factors to hyperbilirubinemia in the human infant is bilirubin production. Determination of the pulmonary excretion rate of carbon monoxide is used to measure indirectly the rate of bilirubin production by estimating the endogenous production of CO, which is formed in equimolar amounts with bilirubin in the catabolism of heme. There has been no study to confirm that complete recovery of CO produced in vivo from the catabolism of known amounts of heme occurs via pulmonary excretion. We report here quantitative determinations of the pulmonary excretion rate of CO in five adult male Wistar rats after injection of known amounts of heme in the form of red blood cells \"damaged\" by incubation in a solution containing a sulfhydryl inhibitor, NEM. The mean recovery of \"extra\" CO above baseline production represented as a molar ratio of extra CO to heme was 0.98 +/- 0.02 (S.E.). Control studies showed no extra CO production after starvation, injection of NEM in an amount comparable to that used in the experimental animals, or injection of undamaged red blood cells."}
{"id": "PMID:479726", "title": "Effects of chemoreceptor and baroreceptor stimulation on the discharge of hypothalamic supraoptic neurones in rats.", "content": "Experiments have been performed to examine the effects of activating the carotid body chemoreceptors and the arterial baroreceptors on the discharge of neurones within the hypothalamic supraoptic nucleus of the rat. Chemoreceptors were activated by intracarotid injection of 0.9% NaCl solution equilibrated with 100% CO2. The baroreceptors of the carotid sinus and aortic arch were activated by raising the blood pressure with an intravenous injection of phenylephrine. Chemoreceptor stimulation activated and baroreceptor stimulation inhibited the discharge of all the phasically discharging neurones tested. Neither stimulus had any consistent effect on non-phasically discharging neurones, although slight inhibition occasionally occurred. Anaesthesia of the carotid bifurcation abolished the effects of cardiovascular stimulation on the supraoptic neurones. Responses resumed when the anaesthesia wore off. However, the anaesthesia also seemed to alter the phasic pattern of discharge. The results are discussed with reference to the influence of the cardiovascular receptors upon the neurones in the supraoptic nucleus, and with reference to possible roles for the cardiovascular reflexes in control of vasopressin secretion.", "contents": "Effects of chemoreceptor and baroreceptor stimulation on the discharge of hypothalamic supraoptic neurones in rats. Experiments have been performed to examine the effects of activating the carotid body chemoreceptors and the arterial baroreceptors on the discharge of neurones within the hypothalamic supraoptic nucleus of the rat. Chemoreceptors were activated by intracarotid injection of 0.9% NaCl solution equilibrated with 100% CO2. The baroreceptors of the carotid sinus and aortic arch were activated by raising the blood pressure with an intravenous injection of phenylephrine. Chemoreceptor stimulation activated and baroreceptor stimulation inhibited the discharge of all the phasically discharging neurones tested. Neither stimulus had any consistent effect on non-phasically discharging neurones, although slight inhibition occasionally occurred. Anaesthesia of the carotid bifurcation abolished the effects of cardiovascular stimulation on the supraoptic neurones. Responses resumed when the anaesthesia wore off. However, the anaesthesia also seemed to alter the phasic pattern of discharge. The results are discussed with reference to the influence of the cardiovascular receptors upon the neurones in the supraoptic nucleus, and with reference to possible roles for the cardiovascular reflexes in control of vasopressin secretion."}
{"id": "PMID:479727", "title": "Prolactin release in parent ring doves after brief exposure to their young.", "content": "A pigeon crop sac radioreceptor assay was used to measure changes in pituitary prolactin levels in parent ring doves of both sexes on the third day after hatching of their young. After a deprivation of 17 h from the squabs, exposure to a 3-day-old squab for 1 h resulted in a significant decrease in the prolactin content of the pituitary gland as compared with levels obtained in control birds deprived of their young for 18 h. No significant sex differences in prolactin levels were observed in either group. Because exposure to the young also promotes prolactin-induced crop sac growth, it appears probable that the squab-induced decrease in prolactin content of the pituitary gland reflects the release of prolactin into the circulation. Accordingly, the environmental regulation of prolactin secretion in parent ring doves appears similar to that observed in lactating mammals.", "contents": "Prolactin release in parent ring doves after brief exposure to their young. A pigeon crop sac radioreceptor assay was used to measure changes in pituitary prolactin levels in parent ring doves of both sexes on the third day after hatching of their young. After a deprivation of 17 h from the squabs, exposure to a 3-day-old squab for 1 h resulted in a significant decrease in the prolactin content of the pituitary gland as compared with levels obtained in control birds deprived of their young for 18 h. No significant sex differences in prolactin levels were observed in either group. Because exposure to the young also promotes prolactin-induced crop sac growth, it appears probable that the squab-induced decrease in prolactin content of the pituitary gland reflects the release of prolactin into the circulation. Accordingly, the environmental regulation of prolactin secretion in parent ring doves appears similar to that observed in lactating mammals."}
{"id": "PMID:479728", "title": "Oestrogenic effects of catechol oestrogens on secretion of prolactin by the pituitary gland and synthesis of DNA by the mammary gland in ovariectomized rats.", "content": "The effects of 2-hydroxyoestradiol-17 beta and 2-hydroxyoestriol (2OH-OE3) on levels of serum prolactin, DNA synthesis by the mammary gland and the weight of the uterus were examined in ovariectomized Sprague-Dawley rats. 2-Hydroxyoestradiol-17 beta had a potent oestrogenic activity in stimulating the secretion of prolactin by the pituitary gland, the synthesis of DNA by the mammary gland and in increasing the weight of the uterus. On the other hand, 2OH-OE3 increased the weight of the uterus only.", "contents": "Oestrogenic effects of catechol oestrogens on secretion of prolactin by the pituitary gland and synthesis of DNA by the mammary gland in ovariectomized rats. The effects of 2-hydroxyoestradiol-17 beta and 2-hydroxyoestriol (2OH-OE3) on levels of serum prolactin, DNA synthesis by the mammary gland and the weight of the uterus were examined in ovariectomized Sprague-Dawley rats. 2-Hydroxyoestradiol-17 beta had a potent oestrogenic activity in stimulating the secretion of prolactin by the pituitary gland, the synthesis of DNA by the mammary gland and in increasing the weight of the uterus. On the other hand, 2OH-OE3 increased the weight of the uterus only."}
{"id": "PMID:479729", "title": "Photoperiodic control of seasonal breeding in the ram: participation of the cranial sympathetic nervous system.", "content": "Four mature Soay rams, cranially sympathectomized by removal of the superior cervical ganglia, were housed alongside four normal rams controlled lighting conditions of alternating 16 week periods of short days of 8 h light: 16 h darkness (8L : 16D) and long days (16L : 8D). The changes in the concentration of FSH, LH, prolactin and testosterone in the plasma, the size of the testes, the intensity of the sexual flush and the sexual and aggressive behaviour of the animals were recorded. While the control rams were able to respond to the artificial lighting conditions with synchronized cycles of reproductive activity, the ganglionectomized animals failed to respond. The treated rams had well-developed testes and relatively high levels of gonadotrophins and testosterone in the blood throughout the experiment. It is concluded that the cranial sympathetic nervous system is involved in the photoperiodic control of seasonal breeding in the ram, probably through its role in the innervation of the pineal gland.", "contents": "Photoperiodic control of seasonal breeding in the ram: participation of the cranial sympathetic nervous system. Four mature Soay rams, cranially sympathectomized by removal of the superior cervical ganglia, were housed alongside four normal rams controlled lighting conditions of alternating 16 week periods of short days of 8 h light: 16 h darkness (8L : 16D) and long days (16L : 8D). The changes in the concentration of FSH, LH, prolactin and testosterone in the plasma, the size of the testes, the intensity of the sexual flush and the sexual and aggressive behaviour of the animals were recorded. While the control rams were able to respond to the artificial lighting conditions with synchronized cycles of reproductive activity, the ganglionectomized animals failed to respond. The treated rams had well-developed testes and relatively high levels of gonadotrophins and testosterone in the blood throughout the experiment. It is concluded that the cranial sympathetic nervous system is involved in the photoperiodic control of seasonal breeding in the ram, probably through its role in the innervation of the pineal gland."}
{"id": "PMID:479731", "title": "Hyperaldosteronism in the sodium-depleted rat: mechanism of aldosterone stimulation by peritoneal dialysis with glucose solution.", "content": "Peritoneal dialysis with 5% glucose solution was carried out in dexamethasone-pretreated rats. Dialysis brought about a severe loss of sodium and a slight loss of potassium into the peritoneal fluid. This kind of sodium depletion took place without any decrease in total body-water space, thus it evoked a severe fall in plasma sodium concentration. Plasma renin activity and the serum concentration of aldosterone increased in response to dialysis. Peak values in renin activity were attained within 60 min, whereas aldosterone concentrations exhibited a continuous rise until at least 120 min. Despite the correlation of renin and aldosterone values, neither the administration of an angiotensin I converting enzyme inhibitor (SQ 20,881) nor the reduction of plasma renin activity by indomethacin could reduce hyperaldosteronism evoked by peritoneal dialysis. Therefore, it is assumed that there is no causal relationship between renin and aldosterone in this kind of acute, severe sodium depletion.", "contents": "Hyperaldosteronism in the sodium-depleted rat: mechanism of aldosterone stimulation by peritoneal dialysis with glucose solution. Peritoneal dialysis with 5% glucose solution was carried out in dexamethasone-pretreated rats. Dialysis brought about a severe loss of sodium and a slight loss of potassium into the peritoneal fluid. This kind of sodium depletion took place without any decrease in total body-water space, thus it evoked a severe fall in plasma sodium concentration. Plasma renin activity and the serum concentration of aldosterone increased in response to dialysis. Peak values in renin activity were attained within 60 min, whereas aldosterone concentrations exhibited a continuous rise until at least 120 min. Despite the correlation of renin and aldosterone values, neither the administration of an angiotensin I converting enzyme inhibitor (SQ 20,881) nor the reduction of plasma renin activity by indomethacin could reduce hyperaldosteronism evoked by peritoneal dialysis. Therefore, it is assumed that there is no causal relationship between renin and aldosterone in this kind of acute, severe sodium depletion."}
{"id": "PMID:479733", "title": "Inhibitory action of follicular fluid on progesterone and prostaglandin synthesis in bovine follicles.", "content": "The present studies examined the inhibitory effect of mid-cycle and preovulatory bovine follicular fluid (bFF) on the secretion of progesterone and prostaglandin F 2 alpha (PGF 2 alpha) by bovine theca and granulosa cells cultured in tissue culture Medium 199. The inhibitory effect of bFF on the secretion of PGF 2 alpha by incubated and cultured hemipituitary glands of male rats was also studied. Addition of 5, 10 or 20% charcoal-extracted, mid-cycle bFF to the culture medium caused a twofold decrease in the accumulation of both progesterone and PGF 2 alpha by the cultured granulosa cells (P less than 0.01). In contrast, when preovulatory bFF was used, there was no inhibitory effect on secretion of progesterone and PGF 2 alpha. The addition of 10% mid-cycle bFF to the culture medium caused a two- to fourfold decrease in the accumulation of both PGF 2 alpha and progesterone by the cultured theca (p less than 0.008). However, in the presence of 1 microgram LH/ml, the inhibitory effect of mid-cycle bFF on progesterone and PGF 2 alpha secretion was abolished. The secretion of PGF 2 alpha was significantly (p less than 0.03) decreased in male rat hemipituitary glands after 5 h of incubation or 18 h of culture. These findings suggest that bFF from mid-cycle follicles inhibits prostaglandin synthetase as well as luteinization. The inhibition disappeared with bFF from preovulatory follicles.", "contents": "Inhibitory action of follicular fluid on progesterone and prostaglandin synthesis in bovine follicles. The present studies examined the inhibitory effect of mid-cycle and preovulatory bovine follicular fluid (bFF) on the secretion of progesterone and prostaglandin F 2 alpha (PGF 2 alpha) by bovine theca and granulosa cells cultured in tissue culture Medium 199. The inhibitory effect of bFF on the secretion of PGF 2 alpha by incubated and cultured hemipituitary glands of male rats was also studied. Addition of 5, 10 or 20% charcoal-extracted, mid-cycle bFF to the culture medium caused a twofold decrease in the accumulation of both progesterone and PGF 2 alpha by the cultured granulosa cells (P less than 0.01). In contrast, when preovulatory bFF was used, there was no inhibitory effect on secretion of progesterone and PGF 2 alpha. The addition of 10% mid-cycle bFF to the culture medium caused a two- to fourfold decrease in the accumulation of both PGF 2 alpha and progesterone by the cultured theca (p less than 0.008). However, in the presence of 1 microgram LH/ml, the inhibitory effect of mid-cycle bFF on progesterone and PGF 2 alpha secretion was abolished. The secretion of PGF 2 alpha was significantly (p less than 0.03) decreased in male rat hemipituitary glands after 5 h of incubation or 18 h of culture. These findings suggest that bFF from mid-cycle follicles inhibits prostaglandin synthetase as well as luteinization. The inhibition disappeared with bFF from preovulatory follicles."}
{"id": "PMID:479734", "title": "Foetal-maternal production and transfer of cortisol during the last days of gestation in the guinea-pig.", "content": "The metabolism and the rate of transfer of cortisol across the placenta in pregnant guinea-pigs and foetuses were studied by constant intravenous infusions of tritium-labelled cortisol. Estimates of endogenous and radioactive plasma cortisol levels were used to calculate the following parameters at four stages before parturition (days 62, 64, 66 and 67; parturition occurring at day 68): metabolic clearance rate; production rate; adrenal secretory rate; transfer rate from mother to foetus and from foetus to mother; irreversible removal rate; the fraction of cortisol derived from the other in the foetal and maternal vascular compartments; the fraction of secreted and recycled cortisol involved in the transfer. The metabolic clearance rate and the rates of production and secretion of cortisol were higher in the mother than in the foetus between days 62 and 67 of gestation. About 90% of the foetal cortisol was of maternal origin. The fraction of maternal cortisol of foetal origin increased in the last days of gestation.", "contents": "Foetal-maternal production and transfer of cortisol during the last days of gestation in the guinea-pig. The metabolism and the rate of transfer of cortisol across the placenta in pregnant guinea-pigs and foetuses were studied by constant intravenous infusions of tritium-labelled cortisol. Estimates of endogenous and radioactive plasma cortisol levels were used to calculate the following parameters at four stages before parturition (days 62, 64, 66 and 67; parturition occurring at day 68): metabolic clearance rate; production rate; adrenal secretory rate; transfer rate from mother to foetus and from foetus to mother; irreversible removal rate; the fraction of cortisol derived from the other in the foetal and maternal vascular compartments; the fraction of secreted and recycled cortisol involved in the transfer. The metabolic clearance rate and the rates of production and secretion of cortisol were higher in the mother than in the foetus between days 62 and 67 of gestation. About 90% of the foetal cortisol was of maternal origin. The fraction of maternal cortisol of foetal origin increased in the last days of gestation."}
{"id": "PMID:479735", "title": "Plasma oxytocin and steroid concentrations during late pregnancy, parturition and lactation in the miniature pig.", "content": "Plasma oxytocin concentrations were measured during late pregnancy, parturition and lactation in the miniature pig. Measurements were made of plasma oestradiol, oestrone and progesterone to determine whether there was any relationship between the concentrations of oxytocin and these steroids in the circulation. Plasma oxytocin concentrations were low or undetectable in late pregnancy. Rises of up to 68.8 mum./ml were seen at the time of delivery of the foetuses and at the expulsion of the placenta. The only steroid that seemed to relat to oxytocin release was progesterone. Oxytocin release was consistently seen when progesterone concentrations had fallen to below 10 ng/ml but no increase in concentration was observed while oestrone and oestradiol increased to their maximum concentrations of 3.86--11.6 and 0.43--0.70 ng/ml respectively. During lactation, when both oestrogen and progesterone concentrations were low, suckling caused the levels of oxytocin to increase to 7.4 muu./ml. These increases were greater during the first 2 weeks of lactation than later.", "contents": "Plasma oxytocin and steroid concentrations during late pregnancy, parturition and lactation in the miniature pig. Plasma oxytocin concentrations were measured during late pregnancy, parturition and lactation in the miniature pig. Measurements were made of plasma oestradiol, oestrone and progesterone to determine whether there was any relationship between the concentrations of oxytocin and these steroids in the circulation. Plasma oxytocin concentrations were low or undetectable in late pregnancy. Rises of up to 68.8 mum./ml were seen at the time of delivery of the foetuses and at the expulsion of the placenta. The only steroid that seemed to relat to oxytocin release was progesterone. Oxytocin release was consistently seen when progesterone concentrations had fallen to below 10 ng/ml but no increase in concentration was observed while oestrone and oestradiol increased to their maximum concentrations of 3.86--11.6 and 0.43--0.70 ng/ml respectively. During lactation, when both oestrogen and progesterone concentrations were low, suckling caused the levels of oxytocin to increase to 7.4 muu./ml. These increases were greater during the first 2 weeks of lactation than later."}
{"id": "PMID:479736", "title": "Hyperaldosteronism in the sodium-depleted rat: mode of aldosterone-stimulating action of frusemide.", "content": "The mechanism of diuretic-induced hyperaldosteronism was examined in dexamethasone-pretreated rats. The diuretic drug frusemide brought about a rapid increase in plasma renin activity and aldosterone concentration in serum. Half an hour after the administration of frusemide the mean concentration of aldosterone was 25 times higher than in vehicle-treated control animals. Administration of SQ 20,881, an inhibitor of angiotensin converting enzyme, prevented the adrenal response to frusemide. The response of aldosterone was completely blocked by indomethacin. This drug reduced renin release and probably also inhibited the effect on the adrenal glands of angiotensin, released in response to frusemide. Our results indicate that the effects of diuretics on the adrenal glomerulosa cells are mediated by the renin-angiotensin system also in the rat. Hyperaldosteronism is dependent on the maintenance of prostaglandin synthesis. ACTH has no essential role in this response.", "contents": "Hyperaldosteronism in the sodium-depleted rat: mode of aldosterone-stimulating action of frusemide. The mechanism of diuretic-induced hyperaldosteronism was examined in dexamethasone-pretreated rats. The diuretic drug frusemide brought about a rapid increase in plasma renin activity and aldosterone concentration in serum. Half an hour after the administration of frusemide the mean concentration of aldosterone was 25 times higher than in vehicle-treated control animals. Administration of SQ 20,881, an inhibitor of angiotensin converting enzyme, prevented the adrenal response to frusemide. The response of aldosterone was completely blocked by indomethacin. This drug reduced renin release and probably also inhibited the effect on the adrenal glands of angiotensin, released in response to frusemide. Our results indicate that the effects of diuretics on the adrenal glomerulosa cells are mediated by the renin-angiotensin system also in the rat. Hyperaldosteronism is dependent on the maintenance of prostaglandin synthesis. ACTH has no essential role in this response."}
{"id": "PMID:479737", "title": "Large-scale preparation of highly purified pyrogen-free human growth hormone for clinical use.", "content": "A method is described for the large-scale isolation of highly purified human growth hormone for therapeutic use. The hormone was extracted from frozen pituitary glands under mildly alkaline conditions. Purification was effected by gel filtration and ion-exchange chromatography. The final product was pyrogen-free and had a potency of 2.5 i.u./mg measured by bioassay against the current international standard. The yield of growth hormone/gland was approximately 5 mg.", "contents": "Large-scale preparation of highly purified pyrogen-free human growth hormone for clinical use. A method is described for the large-scale isolation of highly purified human growth hormone for therapeutic use. The hormone was extracted from frozen pituitary glands under mildly alkaline conditions. Purification was effected by gel filtration and ion-exchange chromatography. The final product was pyrogen-free and had a potency of 2.5 i.u./mg measured by bioassay against the current international standard. The yield of growth hormone/gland was approximately 5 mg."}
{"id": "PMID:479738", "title": "Glucocorticoid and mineralocorticoid pathways in two adrenocortical carcinomas: comparison of the effects of o,p'-dichlorodiphenyldichloroethane, aminoglutethimide and 2-p-aminophenyl-2-phenylethylamine in vitro.", "content": "The synthesis of glucocorticoids and mineralocorticoids in vitro was studied in an adrenocortical carcinoma after alblation from an 11.5-year-old boy. This patient had been unsuccessfully treated with high doses of o,p'-dichlorodiphenyldichloroethane (o,p'-DDD) and aminoglutethimide. These in-vitro results were compared with those obtained with another adrenocortical carcinoma removed from a 26-year-old woman who had received no preoperative treatment. The sensitivity of these adrenocortical carcinomas to o,p'-DDD, aminoglutethimide and 2-(p-aminophenyl)-2-phenylethylamine (SKF 12185) was investigated. Synthesis of cortisol (47%) and corticosterone (45%) in control incubations showed that 11 beta-hydroxylase activity was not affected by the treatment. This explains the raised level of plasma cortisol in the treated child. All three compounds inhibited both 11 beta-hydroxylase and 18-hydroxylase activities up to 95%, depending on the inhibitor. This study shows (a) an inhibitory effect of o,p'-DDD on the steroidogenesis of an adrenocortical carcinoma in vitro, an effect not previously reported in man or laboratory animals, and (b) the in-vitro efficacy of o,p-DDD and aminoglutethimide on corticosteroidogenesis by a carcinoma unresponsive to treatment in vivo. This discrepancy between data obtained in vivo and in vitro could possibly be explained by either an insufficient ratio of ingested dose: tumour mass, or a malabsorption of the drugs in this patient.", "contents": "Glucocorticoid and mineralocorticoid pathways in two adrenocortical carcinomas: comparison of the effects of o,p'-dichlorodiphenyldichloroethane, aminoglutethimide and 2-p-aminophenyl-2-phenylethylamine in vitro. The synthesis of glucocorticoids and mineralocorticoids in vitro was studied in an adrenocortical carcinoma after alblation from an 11.5-year-old boy. This patient had been unsuccessfully treated with high doses of o,p'-dichlorodiphenyldichloroethane (o,p'-DDD) and aminoglutethimide. These in-vitro results were compared with those obtained with another adrenocortical carcinoma removed from a 26-year-old woman who had received no preoperative treatment. The sensitivity of these adrenocortical carcinomas to o,p'-DDD, aminoglutethimide and 2-(p-aminophenyl)-2-phenylethylamine (SKF 12185) was investigated. Synthesis of cortisol (47%) and corticosterone (45%) in control incubations showed that 11 beta-hydroxylase activity was not affected by the treatment. This explains the raised level of plasma cortisol in the treated child. All three compounds inhibited both 11 beta-hydroxylase and 18-hydroxylase activities up to 95%, depending on the inhibitor. This study shows (a) an inhibitory effect of o,p'-DDD on the steroidogenesis of an adrenocortical carcinoma in vitro, an effect not previously reported in man or laboratory animals, and (b) the in-vitro efficacy of o,p-DDD and aminoglutethimide on corticosteroidogenesis by a carcinoma unresponsive to treatment in vivo. This discrepancy between data obtained in vivo and in vitro could possibly be explained by either an insufficient ratio of ingested dose: tumour mass, or a malabsorption of the drugs in this patient."}
{"id": "PMID:479740", "title": "Interaction between inner cell mass and trophectoderm of the mouse blastocyst. II. The fate of the polar trophectoderm.", "content": "Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectoderm cells directly opposite the inner cell mass are the oldest mural cells. However, after implantation polar cells invaginate into the blastocoelic cavity and contribute to the extra-embryonic ectoderm. It is suggested that the morphogenetic changes occurring in the mouse embryo at implantation result from the maintenance of a balance between (a) regional differences in rates of cellular proliferation, and (b) mechanical constraints on the direction in which growth can occur.", "contents": "Interaction between inner cell mass and trophectoderm of the mouse blastocyst. II. The fate of the polar trophectoderm. Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectoderm cells directly opposite the inner cell mass are the oldest mural cells. However, after implantation polar cells invaginate into the blastocoelic cavity and contribute to the extra-embryonic ectoderm. It is suggested that the morphogenetic changes occurring in the mouse embryo at implantation result from the maintenance of a balance between (a) regional differences in rates of cellular proliferation, and (b) mechanical constraints on the direction in which growth can occur."}
{"id": "PMID:479741", "title": "An analysis of the aggregation and morphogenesis of area opaca endoderm cells from the primitive-streak chick embryo.", "content": "The aggregative behaviour and subsequent morphogenesis of extra-embryonic endoderm cells from primitive-streak chick embryos have been investigated. A relatively pure population of area opaca endoderm cells was obtained by differential dissociation, which involves partial separation of epiblast and endoderm cell clumps by sieving through Nitex mesh. For aggregation studies cells were cultured in rotating flasks in Leibovitz (L-15) medium, in saline or in saline supplemented with glucose (1 mg/ml). Aggregation was monitored using the Coulter Counter. In these three media aggregation is rapid; by 10 min an average of 61% of the population had aggregated, to reach a plateau at 30 min when an average percent adhesion value of 83% was obtained. The aggregates in L-15 medium were large and compact. After several days in culture, they cavitated and formed smooth hollow vesicles with thin walls composed of one or a few cell layers. Aggregates formed in PCS were smaller and looser in appearance; the addition of glucose resulted in a certain degree of compaction. Some morphogenesis occurred under these conditions with the aggregates developing numerous irregular cavities. These experiments suggest that some of the factors that affect cell adhesion in early embryonic cells can be studied in vitro. The results also indicate that the ability to cavitate is an intrinsic property of the endoderm cells of the area opaca since this occurs in the absence of epiblast or mesoderm.", "contents": "An analysis of the aggregation and morphogenesis of area opaca endoderm cells from the primitive-streak chick embryo. The aggregative behaviour and subsequent morphogenesis of extra-embryonic endoderm cells from primitive-streak chick embryos have been investigated. A relatively pure population of area opaca endoderm cells was obtained by differential dissociation, which involves partial separation of epiblast and endoderm cell clumps by sieving through Nitex mesh. For aggregation studies cells were cultured in rotating flasks in Leibovitz (L-15) medium, in saline or in saline supplemented with glucose (1 mg/ml). Aggregation was monitored using the Coulter Counter. In these three media aggregation is rapid; by 10 min an average of 61% of the population had aggregated, to reach a plateau at 30 min when an average percent adhesion value of 83% was obtained. The aggregates in L-15 medium were large and compact. After several days in culture, they cavitated and formed smooth hollow vesicles with thin walls composed of one or a few cell layers. Aggregates formed in PCS were smaller and looser in appearance; the addition of glucose resulted in a certain degree of compaction. Some morphogenesis occurred under these conditions with the aggregates developing numerous irregular cavities. These experiments suggest that some of the factors that affect cell adhesion in early embryonic cells can be studied in vitro. The results also indicate that the ability to cavitate is an intrinsic property of the endoderm cells of the area opaca since this occurs in the absence of epiblast or mesoderm."}
{"id": "PMID:479742", "title": "The ultrastructure of the dorsal yolk-free cytoplasm and the immediately surrounding cytoplasm in the symmetrized egg of Xenopus laevis.", "content": "Cytoplasmic segregation and subsequent dorsad displacement of the segregated cytoplasm lead to symmetrization of the egg of Xenopus laevis. At 60 min post-fertilization (p.f.) the 'dorsal yolk-free cytoplasm' (DYFC) is located in the dorso-animal part of the egg. Its ultrastructure and that of the immediately surrounding cytoplasm have been studied with transmission electron microscopy (TEM). The endoplasmic reticulum (ER) within the DYFC consists of single or paired cisternae and many small vesicles, both with moderately dense contents. Numerous particles, presumably ribosomes and glycogen, are present together with many mitochondria and some Golgi structures. The fraction of total yolk-free area occupied by mitochondria in the DYFC is about three times that in the adjacent cytoplasm. The number of cytoplasmic vesicles per unit area of cytoplasm is far larger in the DYFC than in the surrounding area. The morphological characteristics of the DYFC at 60 min p.f. suggest that it represents a region of high metabolic activity. Since it is located in the dorso-animal quadrant of the uncleaved egg, it may be partly responsible for a difference in metabolism between the dorsal and the ventral side of the egg, and hence may play an essential role in the determination of dorso-ventrality.", "contents": "The ultrastructure of the dorsal yolk-free cytoplasm and the immediately surrounding cytoplasm in the symmetrized egg of Xenopus laevis. Cytoplasmic segregation and subsequent dorsad displacement of the segregated cytoplasm lead to symmetrization of the egg of Xenopus laevis. At 60 min post-fertilization (p.f.) the 'dorsal yolk-free cytoplasm' (DYFC) is located in the dorso-animal part of the egg. Its ultrastructure and that of the immediately surrounding cytoplasm have been studied with transmission electron microscopy (TEM). The endoplasmic reticulum (ER) within the DYFC consists of single or paired cisternae and many small vesicles, both with moderately dense contents. Numerous particles, presumably ribosomes and glycogen, are present together with many mitochondria and some Golgi structures. The fraction of total yolk-free area occupied by mitochondria in the DYFC is about three times that in the adjacent cytoplasm. The number of cytoplasmic vesicles per unit area of cytoplasm is far larger in the DYFC than in the surrounding area. The morphological characteristics of the DYFC at 60 min p.f. suggest that it represents a region of high metabolic activity. Since it is located in the dorso-animal quadrant of the uncleaved egg, it may be partly responsible for a difference in metabolism between the dorsal and the ventral side of the egg, and hence may play an essential role in the determination of dorso-ventrality."}
{"id": "PMID:479743", "title": "Cell number in relation to primary pattern formation in the embryo of Xenopus laevis. I. The cell cycle during new pattern formation in response to implanted organizers.", "content": "Results are presented which offer strong evidence that extensive alteration of the fates of embryonic Xenopus cells occurs independently of the schedule of cell division, after operations which lead to a doubling of the axial pattern of mesodermal differentiation in the gastrula. The experimental strategy was to make estimates of total mesodermal cell numbers and mitotic index in closely matched sets, each of three synchronous sibling embryos, fixed during the ten hours following the close of gastrulation. Within each set two embryos, an unoperated control and a sham-operated embryo whose own dorsal-lip (organizer) cells had been replaced with an equivalent graft, were developing normally. The third, experimental embryo had received an organizer implant to replace an equivalent number of cells from its ventral marginal zone, and was thus developing two axial mesodermal patterns of differentiation in relation to two dorsal midlines, the extra pattern embracing much host tissue. Mitotic index was also determined, in specific regions and throughout the mesoderm, in similar sets of embryos but at mid-gastrula stages. The conclusions are justified by the results of a control investigation which show that there is normally no difference in cell cycle time along the presumptive dorso-ventral mesodermal, dimension, during the interval between time of operations and the determination of pattern. The lack of any enhancement of mesodermal cell number in late embryos with dual axia patterns, or intervening enhancement of mitotic index in younger operated embryos, thus suggests that new patterns may be determined in the Xenopus gastrula without generation of extra cells. The results are discussed in relation to recent ideas about pattern formation, and the concepts of morphallaxis and epimorphosis.", "contents": "Cell number in relation to primary pattern formation in the embryo of Xenopus laevis. I. The cell cycle during new pattern formation in response to implanted organizers. Results are presented which offer strong evidence that extensive alteration of the fates of embryonic Xenopus cells occurs independently of the schedule of cell division, after operations which lead to a doubling of the axial pattern of mesodermal differentiation in the gastrula. The experimental strategy was to make estimates of total mesodermal cell numbers and mitotic index in closely matched sets, each of three synchronous sibling embryos, fixed during the ten hours following the close of gastrulation. Within each set two embryos, an unoperated control and a sham-operated embryo whose own dorsal-lip (organizer) cells had been replaced with an equivalent graft, were developing normally. The third, experimental embryo had received an organizer implant to replace an equivalent number of cells from its ventral marginal zone, and was thus developing two axial mesodermal patterns of differentiation in relation to two dorsal midlines, the extra pattern embracing much host tissue. Mitotic index was also determined, in specific regions and throughout the mesoderm, in similar sets of embryos but at mid-gastrula stages. The conclusions are justified by the results of a control investigation which show that there is normally no difference in cell cycle time along the presumptive dorso-ventral mesodermal, dimension, during the interval between time of operations and the determination of pattern. The lack of any enhancement of mesodermal cell number in late embryos with dual axia patterns, or intervening enhancement of mitotic index in younger operated embryos, thus suggests that new patterns may be determined in the Xenopus gastrula without generation of extra cells. The results are discussed in relation to recent ideas about pattern formation, and the concepts of morphallaxis and epimorphosis."}
{"id": "PMID:479744", "title": "Bristle patterns and compartment boundaries in the tarsi of Drosophila.", "content": "We describe cell lineage of the tarsus of wild-type Drosophila. Large Minute+ clones were made to map the position of the antero-posterior compartment boundary in all three tarsi. The tarsus is mirror symmetric, but the compartment boundary does not coincide with the mirror plane. This boundary runs along the dorsal and the ventral rows of bristles which are immediately posterior to the mirror plane; elements in these rows being made by both anterior and posterior polyclones. The provenance of bristles and bracts suggests that the bristle cells move into their final positions. The homoeotic mutation engrailed affects only the posterior compartments of all three tarsi. The mutations bithorax and postbithorax affect only the anterior and posterior compartments of the third legs, respectively, transforming them into homologous compartments of the second leg. These results support the selector gene model of development (Garcia-Bellido, 1975) and emphasize that collaboration between polyclones is important in pattern formation.", "contents": "Bristle patterns and compartment boundaries in the tarsi of Drosophila. We describe cell lineage of the tarsus of wild-type Drosophila. Large Minute+ clones were made to map the position of the antero-posterior compartment boundary in all three tarsi. The tarsus is mirror symmetric, but the compartment boundary does not coincide with the mirror plane. This boundary runs along the dorsal and the ventral rows of bristles which are immediately posterior to the mirror plane; elements in these rows being made by both anterior and posterior polyclones. The provenance of bristles and bracts suggests that the bristle cells move into their final positions. The homoeotic mutation engrailed affects only the posterior compartments of all three tarsi. The mutations bithorax and postbithorax affect only the anterior and posterior compartments of the third legs, respectively, transforming them into homologous compartments of the second leg. These results support the selector gene model of development (Garcia-Bellido, 1975) and emphasize that collaboration between polyclones is important in pattern formation."}
{"id": "PMID:479745", "title": "The activity of cholinesterases during the development of Xenopus laevis.", "content": "The activity of cholinesterases during the early development of Xenopus laevis has been examined, and the activity of acetylcholinesterase in particular has been distinguished from other cholinesterases. In contrast to some earlier findings, the activity of acetylcholinesterase is low at early stages and gradually increases during development. Possible reasons for the differences between the earlier results and those reported here are discussed.", "contents": "The activity of cholinesterases during the development of Xenopus laevis. The activity of cholinesterases during the early development of Xenopus laevis has been examined, and the activity of acetylcholinesterase in particular has been distinguished from other cholinesterases. In contrast to some earlier findings, the activity of acetylcholinesterase is low at early stages and gradually increases during development. Possible reasons for the differences between the earlier results and those reported here are discussed."}
{"id": "PMID:479746", "title": "Factors regulating the degree and extent of experimental chimaerism in the mouse.", "content": "The degree of chimaerism of the coat, bone marrow and cornea in mouse chimaeras obtained by aggregation of an albino inbred embryo of A strain with an A X CBA-T6T6 hybrid was investigated. Out of 21 individuals born, 18 were chimaeras. Statistically significant correlation has been demonstrated between chimaerism of the coat, bone marrow and the cornea. The contribution of the two components appears to be affected by two factors: first, variable participation of the two components inthe embryonic ectoderm of the chimaeric egg cyclinder, and second, the rate of proliferation of the two cell lines. A photometric method is described which permits the quantitative evaluation of coat chimaerism. The method is sensitive and reliable.", "contents": "Factors regulating the degree and extent of experimental chimaerism in the mouse. The degree of chimaerism of the coat, bone marrow and cornea in mouse chimaeras obtained by aggregation of an albino inbred embryo of A strain with an A X CBA-T6T6 hybrid was investigated. Out of 21 individuals born, 18 were chimaeras. Statistically significant correlation has been demonstrated between chimaerism of the coat, bone marrow and the cornea. The contribution of the two components appears to be affected by two factors: first, variable participation of the two components inthe embryonic ectoderm of the chimaeric egg cyclinder, and second, the rate of proliferation of the two cell lines. A photometric method is described which permits the quantitative evaluation of coat chimaerism. The method is sensitive and reliable."}
{"id": "PMID:479747", "title": "The mechanism of somite segmentation in the chick embryo.", "content": "The segmentation of somites in the chick embryo has been studied by transmission and scanning electron microscopy (stages 8-14). The segmental plate mesoderm consists of loosely arranged mesenchymal cells, whereas the newly formed somites are composed of elongated, spindle-shaped cells arranged radially around a lumen, the myocoele. The diamter of each somite is thus two cells plus the myocoele. Two major factors appear to be responsible for the change in cell shape at segmentation: (1) Each prospective somite cell becomes anchored at one end to the adjacent epithelia (i.e. the neural tube, the notochord, the ectoderm, the endoderm or the aorta) by means of collagen fibrils. These fibrils are already present in the segmental plate before the somites begin to form. (2) A change in cell-to-cell adhesiveness causes the free ends of these cells to adhere to one another. (Bellairs, Curtis & Sanders, 1978). This adhesion is then supplemented by the development of tight junctions proximally in the somite. Because it is anchored at both ends, each somite cell is under tension in much the same way as a fibroblast cell in tissue culture is under tension. Each somite cell therefore becomes elongated and the somite as a whole accommodates its general shape to that of the space available between the adjacent tissues. The arrangement of the cells in the more differentiated somites (stages 17-18) has also been examined and it has been found that the chick resembles Xenopus in that the myotome cells undergo rotation and become orientated in an anteroposterior direction.", "contents": "The mechanism of somite segmentation in the chick embryo. The segmentation of somites in the chick embryo has been studied by transmission and scanning electron microscopy (stages 8-14). The segmental plate mesoderm consists of loosely arranged mesenchymal cells, whereas the newly formed somites are composed of elongated, spindle-shaped cells arranged radially around a lumen, the myocoele. The diamter of each somite is thus two cells plus the myocoele. Two major factors appear to be responsible for the change in cell shape at segmentation: (1) Each prospective somite cell becomes anchored at one end to the adjacent epithelia (i.e. the neural tube, the notochord, the ectoderm, the endoderm or the aorta) by means of collagen fibrils. These fibrils are already present in the segmental plate before the somites begin to form. (2) A change in cell-to-cell adhesiveness causes the free ends of these cells to adhere to one another. (Bellairs, Curtis & Sanders, 1978). This adhesion is then supplemented by the development of tight junctions proximally in the somite. Because it is anchored at both ends, each somite cell is under tension in much the same way as a fibroblast cell in tissue culture is under tension. Each somite cell therefore becomes elongated and the somite as a whole accommodates its general shape to that of the space available between the adjacent tissues. The arrangement of the cells in the more differentiated somites (stages 17-18) has also been examined and it has been found that the chick resembles Xenopus in that the myotome cells undergo rotation and become orientated in an anteroposterior direction."}
{"id": "PMID:479748", "title": "Somite formation in the early chick embryo following grafts of Hensen's node.", "content": "Quail grafts of Hensen's node were examined for their potential to induce somites in chick blastoderms. The origin of the structures induced depended on the distance of the graft from the host's midline. Nodes placed at the margin of the area pellucida resulted in structures differentiated from the cells of the graft, whereas medially the graft organized host cells to form rows of somites. The results are discussed in terms of competence of graft and host mesenchyme and a positional signal from the node.", "contents": "Somite formation in the early chick embryo following grafts of Hensen's node. Quail grafts of Hensen's node were examined for their potential to induce somites in chick blastoderms. The origin of the structures induced depended on the distance of the graft from the host's midline. Nodes placed at the margin of the area pellucida resulted in structures differentiated from the cells of the graft, whereas medially the graft organized host cells to form rows of somites. The results are discussed in terms of competence of graft and host mesenchyme and a positional signal from the node."}
{"id": "PMID:479749", "title": "Effects of colchicine on spermiogenesis in the mouse.", "content": "The effects of colchicine on spermiogenesis in the mouse were examined. Mice were injected intratesticularly with colchicine in concentrations of 10(-4) M to 10(-6) M for various periods of time. The ultrastructure of the testes was examined and abnormal sperm in the epididymis were scored. Colchicine degraded microtubules in the testis and the treatment caused abnormalities of the head and acrosome of testicular spermatids and also resulted in an increase on the number of epididymal sperm showing abnormal head structure by 5 days after treatment of the testes. These results suggest that structurally intact microtubules are essential for the normal morphogenetic processes of spermiogenesis.", "contents": "Effects of colchicine on spermiogenesis in the mouse. The effects of colchicine on spermiogenesis in the mouse were examined. Mice were injected intratesticularly with colchicine in concentrations of 10(-4) M to 10(-6) M for various periods of time. The ultrastructure of the testes was examined and abnormal sperm in the epididymis were scored. Colchicine degraded microtubules in the testis and the treatment caused abnormalities of the head and acrosome of testicular spermatids and also resulted in an increase on the number of epididymal sperm showing abnormal head structure by 5 days after treatment of the testes. These results suggest that structurally intact microtubules are essential for the normal morphogenetic processes of spermiogenesis."}
{"id": "PMID:479750", "title": "[The role of serotonin and catecholamines in the regeneration of the Planaria Polycelis tenvis].", "content": "During traumatic regeneration of Planaria Polycelis tenuis, determination of serotonin, noradrenaline and dopamine levels revealed important variations of serotonin and catecholamines from the time of excision. The use of specific inhibitors of these hormones delayed regeneration whereas simultaneous addition of an hormone and its antagonist restores a standard time of regeneration. Serotonin acts through adenylate cyclase system. Results allow us to assume that dopamine acts through the same mechanism. The action of noradrenaline is so far not elucidated although propranolol (beta antagonist) delays or inhibits completely regeneration.", "contents": "[The role of serotonin and catecholamines in the regeneration of the Planaria Polycelis tenvis]. During traumatic regeneration of Planaria Polycelis tenuis, determination of serotonin, noradrenaline and dopamine levels revealed important variations of serotonin and catecholamines from the time of excision. The use of specific inhibitors of these hormones delayed regeneration whereas simultaneous addition of an hormone and its antagonist restores a standard time of regeneration. Serotonin acts through adenylate cyclase system. Results allow us to assume that dopamine acts through the same mechanism. The action of noradrenaline is so far not elucidated although propranolol (beta antagonist) delays or inhibits completely regeneration."}
{"id": "PMID:479751", "title": "Latent effects on in vitro development following cytochalasin B treatment of 8-cell mouse embryos.", "content": "Eight-cell mouse embryos when treated with 4.0 microgram/ml cytochalasin B (CB) in vitro undergo a reversible developmental arrest. Upon rinsing of embryos and subsequent culture in control medium, normal morphogenetic processes such as compaction of 8-cell embryos, cavitation, and post-blastocyst attachment and outgrowth are restored. However, the effects of CB on mouse embryos are not completely reversible; latent post-blastocyst defects become increasingly more prevalent as CB treatment duration increases. The present study was conducted to quantitatively determine latent effects of CB on post-blastocyst embryos by comparing their ability to attach and to sustain the growth and differentiation of ICM and trophoblast tissues. Groups of 8-cell embryos were cultured in Brinster's BMOC-3 medium containing 4.0 microgram/ml cytochalasin B for 6, 12, 18, and 24 h. Following treatment, embryos were rinsed and cultured until 190 h post coitum (h.p.c.) in Eagle's MEM/10% fetal calf serum modified to contain optimal levels of essential amino acids. Blastocysts generally attached to the surface of the plastic substratum by 120 h.p.c. At selected time periods after attachment (130, 160, and 190 h.p.c.), embryos were scored for outgrowth size, ICM size, extent of peripheral hyaloplasmic fan, and number of trophoblast nuclei per outgrowth. Analyses of variance (ANOVAs) were conducted for each of the four parameters listed above. Rates of attachment were analyzed by chi2 test. Results show that the treatments affect (P less than 0.01) embryo attachment, number of trophoblast nuclei per outgrowth, hyaloplasmic fan production, and ICM growth in a duration-dependent manner. Interestingly, since treatment effects on outgrowth areas are nonsignificant apparently CB does not significantly change total outgrowth area. But CB treatment does cause abnormal fan production and decreased trophoblast nuclei numbers. However, trophoblast cells are apparently more resistant than ICM to CB as is evident by the high incidence of trophoblast outgrowths devoid of ICM. CB (4.0 microgram/ml) treatments at 8-cell stages for relatively short durations (6 and 12 h) induce latent effects on post-blastocyst embryos. Finally, there exists a definite 4.0 microgram/ml CB duration response over the 68-190 h.p.c. observation interval.", "contents": "Latent effects on in vitro development following cytochalasin B treatment of 8-cell mouse embryos. Eight-cell mouse embryos when treated with 4.0 microgram/ml cytochalasin B (CB) in vitro undergo a reversible developmental arrest. Upon rinsing of embryos and subsequent culture in control medium, normal morphogenetic processes such as compaction of 8-cell embryos, cavitation, and post-blastocyst attachment and outgrowth are restored. However, the effects of CB on mouse embryos are not completely reversible; latent post-blastocyst defects become increasingly more prevalent as CB treatment duration increases. The present study was conducted to quantitatively determine latent effects of CB on post-blastocyst embryos by comparing their ability to attach and to sustain the growth and differentiation of ICM and trophoblast tissues. Groups of 8-cell embryos were cultured in Brinster's BMOC-3 medium containing 4.0 microgram/ml cytochalasin B for 6, 12, 18, and 24 h. Following treatment, embryos were rinsed and cultured until 190 h post coitum (h.p.c.) in Eagle's MEM/10% fetal calf serum modified to contain optimal levels of essential amino acids. Blastocysts generally attached to the surface of the plastic substratum by 120 h.p.c. At selected time periods after attachment (130, 160, and 190 h.p.c.), embryos were scored for outgrowth size, ICM size, extent of peripheral hyaloplasmic fan, and number of trophoblast nuclei per outgrowth. Analyses of variance (ANOVAs) were conducted for each of the four parameters listed above. Rates of attachment were analyzed by chi2 test. Results show that the treatments affect (P less than 0.01) embryo attachment, number of trophoblast nuclei per outgrowth, hyaloplasmic fan production, and ICM growth in a duration-dependent manner. Interestingly, since treatment effects on outgrowth areas are nonsignificant apparently CB does not significantly change total outgrowth area. But CB treatment does cause abnormal fan production and decreased trophoblast nuclei numbers. However, trophoblast cells are apparently more resistant than ICM to CB as is evident by the high incidence of trophoblast outgrowths devoid of ICM. CB (4.0 microgram/ml) treatments at 8-cell stages for relatively short durations (6 and 12 h) induce latent effects on post-blastocyst embryos. Finally, there exists a definite 4.0 microgram/ml CB duration response over the 68-190 h.p.c. observation interval."}
{"id": "PMID:479758", "title": "The role of monocytes in serum sickness nephritis.", "content": "We have investigated the pathogenesis of glomerular hypercellularity seen in acute serum sickness nephritis induced in rabbits with bovine serum albumin (BSA). The increase in cellularity began with the first stages of immune clearance of BSA, with a peak cellularity occuring at the time of onset of proteinuria. Although there was a significant increase in the fraction of glomerular cells incorporating [3H]thymidine, first seen at the onset of proteinuria, this increase occurred too late and was too small to explain the observed rate of increase in glomerular cellularity. On the other hand, a striking monocytic infiltration of the glomeruli was documented by electron microscopy and by staining for nonspecific esterase. This monocytic infiltration paralleled the observed course of glomerular hypercellularity and was quantitatively sufficient to explain the total increase seen. It appears, therefore, that glomerular hypercellularity seen in this model is principally a result of monocyte infiltration.", "contents": "The role of monocytes in serum sickness nephritis. We have investigated the pathogenesis of glomerular hypercellularity seen in acute serum sickness nephritis induced in rabbits with bovine serum albumin (BSA). The increase in cellularity began with the first stages of immune clearance of BSA, with a peak cellularity occuring at the time of onset of proteinuria. Although there was a significant increase in the fraction of glomerular cells incorporating [3H]thymidine, first seen at the onset of proteinuria, this increase occurred too late and was too small to explain the observed rate of increase in glomerular cellularity. On the other hand, a striking monocytic infiltration of the glomeruli was documented by electron microscopy and by staining for nonspecific esterase. This monocytic infiltration paralleled the observed course of glomerular hypercellularity and was quantitatively sufficient to explain the total increase seen. It appears, therefore, that glomerular hypercellularity seen in this model is principally a result of monocyte infiltration."}
{"id": "PMID:479759", "title": "Tumor necrosis serum induces a serologically distinct population of NK cells.", "content": "Murine spleen cells generate nonspecific cytotoxic cells, referred to as natural killer (NK) cells, within 4 d of incubation in Mishell-Dutton cultures. This NK cell type does not arise in cultures of BALB/c.nu spleen cells or in cultures of T-cell depleted C57BL/6 spleen cells, indicating that its activation depends on T cells. Another type of NK cells is induced by tumor necrosis serum in murine spleen-cell cultures. It arises within 24 h and its activation does not depend on T cells. This cell type (and its precursor) expresses the recently discovered cell-surface marker Qa5 (controlled by the Q region of chromosome 17) that distinguishes this NK cell from the NK cell that depends for its activation on thymic function. Qa5+ NK cells are also induced by interferon.", "contents": "Tumor necrosis serum induces a serologically distinct population of NK cells. Murine spleen cells generate nonspecific cytotoxic cells, referred to as natural killer (NK) cells, within 4 d of incubation in Mishell-Dutton cultures. This NK cell type does not arise in cultures of BALB/c.nu spleen cells or in cultures of T-cell depleted C57BL/6 spleen cells, indicating that its activation depends on T cells. Another type of NK cells is induced by tumor necrosis serum in murine spleen-cell cultures. It arises within 24 h and its activation does not depend on T cells. This cell type (and its precursor) expresses the recently discovered cell-surface marker Qa5 (controlled by the Q region of chromosome 17) that distinguishes this NK cell from the NK cell that depends for its activation on thymic function. Qa5+ NK cells are also induced by interferon."}
{"id": "PMID:479760", "title": "Lysis of measles virus-infected cells by the purified cytolytic alternative complement pathway and antibody.", "content": "The dependence of antibody-and-complement-mediated lysis of virus-infected cells on the alternative pathway was examined utilizing the isolated cytolytic alternative pathway--a system consisting of the six purified proteins of the alternative pathway of activation (C3, factors B and D, beta 1H, C3b inactivator and properdin), and the five proteins of the membrane attack pathway (C5--9) of complement. HeLa cells acutely infected with measles virus were lysed by anti-viral IgG and the isolated cytolytic alternative pathway with an efficiency comparable to whole human serum. IgG and its F(ab')2 fragment were equally effective in inducing lysis by the isolated cytolytic alternative pathway, binding of approximately equal to 5 X 10(7) molecules per cell being required for 50% lysis; in contrast, no lysis occurred when equivalen or greater amounts of Fab' were bound to the virus-infected cell. Properdin was required for lysis. No lysis occurred if properdin was deleted from the isolated cytolytic alternative pathway, and lysis was diminished by 80% in properdin-depleted serum. Uptake of [125I]C3b from the isolated alternative pathway onto measles virus-infected cells occurred in the absence of properdin, but was accelerated in the presence of properdin. The 11 proteins of the isolated cytolytic alternative pathway are thus sufficient for lysis of measles virus-infected cells bearing anti-viral IgG or F(ab')2 without any other serum protein.", "contents": "Lysis of measles virus-infected cells by the purified cytolytic alternative complement pathway and antibody. The dependence of antibody-and-complement-mediated lysis of virus-infected cells on the alternative pathway was examined utilizing the isolated cytolytic alternative pathway--a system consisting of the six purified proteins of the alternative pathway of activation (C3, factors B and D, beta 1H, C3b inactivator and properdin), and the five proteins of the membrane attack pathway (C5--9) of complement. HeLa cells acutely infected with measles virus were lysed by anti-viral IgG and the isolated cytolytic alternative pathway with an efficiency comparable to whole human serum. IgG and its F(ab')2 fragment were equally effective in inducing lysis by the isolated cytolytic alternative pathway, binding of approximately equal to 5 X 10(7) molecules per cell being required for 50% lysis; in contrast, no lysis occurred when equivalen or greater amounts of Fab' were bound to the virus-infected cell. Properdin was required for lysis. No lysis occurred if properdin was deleted from the isolated cytolytic alternative pathway, and lysis was diminished by 80% in properdin-depleted serum. Uptake of [125I]C3b from the isolated alternative pathway onto measles virus-infected cells occurred in the absence of properdin, but was accelerated in the presence of properdin. The 11 proteins of the isolated cytolytic alternative pathway are thus sufficient for lysis of measles virus-infected cells bearing anti-viral IgG or F(ab')2 without any other serum protein."}
{"id": "PMID:479761", "title": "Target-effector interaction in the human and murine natural killer system: specificity and xenogeneic reactivity of the solubilized natural killer-target structure complex and its loss in a somatic cell hybrid.", "content": "Preincubation of natural killer (NK) cells with electrophoresis purified proteins from a variety of NK-sensitive murine and human tumor cells specifically prevented subsequent binding to the intact, homologous target cell. The NK-target structures (NK-TS) consisted of some or all of four characteristic molecular species, tentatively assigned molecular weights of 140K, 160K, 190K, and 240K (+/-10K) based on electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels. When these NK-TS molecules were compared in cross-inhibition assays, the large 240K molecule most often carried the unique NK specificity, whereas the smaller 140K molecules cross-reacted between YAC, 136-6 and X-63 in the mouse and between Molt-4 and K562 in the human. Mouse NK cells recognised a different spectrum of NK-TS molecules than human NK cells. The control of NK-TS expression was partially revealed in a cloned, somatic cell hybrid bwtween an NK sensitive (YAC-IR) and insensitive (A9HT) cell line. The hybrid did not express NK-TS and did not bind to NK cells which is in accordance with negative NK cytolytic results previously reported. Although unique specificities are carried by some of the multiple NK-TS protein molecules, cross-reactions were widespread. These observations taken together suggest that the NK cell is polyspecific and has some heterogeneity in the recognition structure although much less than would be expected of an antibody-combining site.", "contents": "Target-effector interaction in the human and murine natural killer system: specificity and xenogeneic reactivity of the solubilized natural killer-target structure complex and its loss in a somatic cell hybrid. Preincubation of natural killer (NK) cells with electrophoresis purified proteins from a variety of NK-sensitive murine and human tumor cells specifically prevented subsequent binding to the intact, homologous target cell. The NK-target structures (NK-TS) consisted of some or all of four characteristic molecular species, tentatively assigned molecular weights of 140K, 160K, 190K, and 240K (+/-10K) based on electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels. When these NK-TS molecules were compared in cross-inhibition assays, the large 240K molecule most often carried the unique NK specificity, whereas the smaller 140K molecules cross-reacted between YAC, 136-6 and X-63 in the mouse and between Molt-4 and K562 in the human. Mouse NK cells recognised a different spectrum of NK-TS molecules than human NK cells. The control of NK-TS expression was partially revealed in a cloned, somatic cell hybrid bwtween an NK sensitive (YAC-IR) and insensitive (A9HT) cell line. The hybrid did not express NK-TS and did not bind to NK cells which is in accordance with negative NK cytolytic results previously reported. Although unique specificities are carried by some of the multiple NK-TS protein molecules, cross-reactions were widespread. These observations taken together suggest that the NK cell is polyspecific and has some heterogeneity in the recognition structure although much less than would be expected of an antibody-combining site."}
{"id": "PMID:479762", "title": "Cell surface antigens of human renal cancer defined by autologous typing.", "content": "Sera from 28 patients with renal cancer were tested for reactivity with surface antigens of cultured autologous renal cancer cells. Four serological assays were used to survey sera for autologous antibody. Immune adherence, protein A, and C3-mixed hemadsorption assays detected reactivity in a high percentage of patients (80-100%), whereas mixed hemadsorption assays were negative with sera from all but one patient. Reactive sera from six patients were analyzed by absorption tests with autologous, allogeneic, and restricted to autologous renal cancer cells; class 2 antigens, present on certain allogeneic renal and nonrenal cancer cells; and class 3 antigens, found on a wide variety of normal and malignant cell types. The sera of one patient detected class 1, 2, and 3 antigens, the sera of three patients detected class 2 antigens, and the sera of two patients detected class 3 antigens. This analysis of renal cancer, with the recognition of three classes of surface antigens recognized by autologous sera, resembles the results of autologous typing of three other human malignancies: malignant melanoma, acute leukemia, and astrocytoma. Evidence provided by autologous typing of these cancers indicates that class 1 and class 2 antigens are tumor-restricted and that under certain circumstances these antigens are immunogenic for the autologous host.", "contents": "Cell surface antigens of human renal cancer defined by autologous typing. Sera from 28 patients with renal cancer were tested for reactivity with surface antigens of cultured autologous renal cancer cells. Four serological assays were used to survey sera for autologous antibody. Immune adherence, protein A, and C3-mixed hemadsorption assays detected reactivity in a high percentage of patients (80-100%), whereas mixed hemadsorption assays were negative with sera from all but one patient. Reactive sera from six patients were analyzed by absorption tests with autologous, allogeneic, and restricted to autologous renal cancer cells; class 2 antigens, present on certain allogeneic renal and nonrenal cancer cells; and class 3 antigens, found on a wide variety of normal and malignant cell types. The sera of one patient detected class 1, 2, and 3 antigens, the sera of three patients detected class 2 antigens, and the sera of two patients detected class 3 antigens. This analysis of renal cancer, with the recognition of three classes of surface antigens recognized by autologous sera, resembles the results of autologous typing of three other human malignancies: malignant melanoma, acute leukemia, and astrocytoma. Evidence provided by autologous typing of these cancers indicates that class 1 and class 2 antigens are tumor-restricted and that under certain circumstances these antigens are immunogenic for the autologous host."}
{"id": "PMID:479763", "title": "Detection of a mediator derived from endotoxin-stimulated macrohpages that induces the acute phase serum amyloid A response in mice.", "content": "The mechanism by which LPS stimulates an acute phase serum amyloid A (SAA) response in C3H mice has been studied. A factor (SAA inducer) appears in the blood of C3H/HeN (lipopolysaccharide [LPS]-sensitive) mice approximately 1 h after administration of LPS, which, when passively administered, can induce C3H/HeJ mice to produce SAA although they are resistant to the LPS itself. SAA inducer has been detected in the culture medium of LPS treated C3H/HeN macrophages but not spleen cells. Thus, two stages in the induction of the acute phase SAA response are now recognized: a latent period of 2-3 h during which the SAA concentration remains at baseline values and in which SAA inducer appears, and the period of synthesis of SAA which lasts for approoximately 24 h past induction. It is proposed that a macrophage response to LPS is responsible for production of the serum mediator which induces SAA synthesis.", "contents": "Detection of a mediator derived from endotoxin-stimulated macrohpages that induces the acute phase serum amyloid A response in mice. The mechanism by which LPS stimulates an acute phase serum amyloid A (SAA) response in C3H mice has been studied. A factor (SAA inducer) appears in the blood of C3H/HeN (lipopolysaccharide [LPS]-sensitive) mice approximately 1 h after administration of LPS, which, when passively administered, can induce C3H/HeJ mice to produce SAA although they are resistant to the LPS itself. SAA inducer has been detected in the culture medium of LPS treated C3H/HeN macrophages but not spleen cells. Thus, two stages in the induction of the acute phase SAA response are now recognized: a latent period of 2-3 h during which the SAA concentration remains at baseline values and in which SAA inducer appears, and the period of synthesis of SAA which lasts for approoximately 24 h past induction. It is proposed that a macrophage response to LPS is responsible for production of the serum mediator which induces SAA synthesis."}
{"id": "PMID:479764", "title": "Human complement in thrombin-mediated platelet function: uptake of the C5b-9 complex.", "content": "Thrombin-mediated platelet aggregation and release is enhanced by the presence of C3, C5, C6, C7, C8, and C9 of human complement. The interaction of thrombin with its receptor on the platelet membrane initiates activation of complement on the platelet surface. Trypsin-mediated platelet function is not enhanced by the addition of complement, probably because trypsin has no receptor on the platelet surface so activation of complement is triggered in the fluid phase and not on the platelet surface. Activation of complement by thrombin led to production of dimers of the C5b-9 complex on the platelet surface. These complexes were eluted from the platelet membrane and were identified physicochemically and morphologically. The mechanism of complement-induced enhancement of platelet function is not clear, however, it probably is mediated via the arachidonic acid transormation pathway because this activity was blocked by known inhibitors of cyclo-oxygenase, namely, aspirin and indomethacin.", "contents": "Human complement in thrombin-mediated platelet function: uptake of the C5b-9 complex. Thrombin-mediated platelet aggregation and release is enhanced by the presence of C3, C5, C6, C7, C8, and C9 of human complement. The interaction of thrombin with its receptor on the platelet membrane initiates activation of complement on the platelet surface. Trypsin-mediated platelet function is not enhanced by the addition of complement, probably because trypsin has no receptor on the platelet surface so activation of complement is triggered in the fluid phase and not on the platelet surface. Activation of complement by thrombin led to production of dimers of the C5b-9 complex on the platelet surface. These complexes were eluted from the platelet membrane and were identified physicochemically and morphologically. The mechanism of complement-induced enhancement of platelet function is not clear, however, it probably is mediated via the arachidonic acid transormation pathway because this activity was blocked by known inhibitors of cyclo-oxygenase, namely, aspirin and indomethacin."}
{"id": "PMID:479765", "title": "Site of binding of mouse IgG2b to the Fc receptor on mouse macrophages.", "content": "Three mouse immunoglobulins with altered heavy chains have been used to study the specificity of the mouse IgG2b Fc receptor on mouse macrophages. These immunoglobulins were synthesized by variant clones derived from the MPC 11, IgG2b-producing mouse myeloma cell line. One variant, whose Fc receptor. A second variant, which makes a short heavy chain lacking the CH3 domain, binds specifically to the IgG2b Fc receptor. The third variant makes a hybrid IgG2b-IgG2a heavy chain whose CH3 domain is enterely IgG2a-like and binds to both IgG2a and IgG2b Fc receptors. These data suggest that the binding of mouse IgG2b immunoglobulins to the mouse macrophage Fc receptor involves a site within the CH2 domain and indicate that immunoglobulins with altered heavy chains are a useful tool to probe Fc receptors.", "contents": "Site of binding of mouse IgG2b to the Fc receptor on mouse macrophages. Three mouse immunoglobulins with altered heavy chains have been used to study the specificity of the mouse IgG2b Fc receptor on mouse macrophages. These immunoglobulins were synthesized by variant clones derived from the MPC 11, IgG2b-producing mouse myeloma cell line. One variant, whose Fc receptor. A second variant, which makes a short heavy chain lacking the CH3 domain, binds specifically to the IgG2b Fc receptor. The third variant makes a hybrid IgG2b-IgG2a heavy chain whose CH3 domain is enterely IgG2a-like and binds to both IgG2a and IgG2b Fc receptors. These data suggest that the binding of mouse IgG2b immunoglobulins to the mouse macrophage Fc receptor involves a site within the CH2 domain and indicate that immunoglobulins with altered heavy chains are a useful tool to probe Fc receptors."}
{"id": "PMID:479766", "title": "[Determination of Fe, Cu and Zn by a mechanised micro-method of flame photometry (author's transl)].", "content": "By sample application in measured quantities (\"injection method\") the quality of serum required for the determination of trace elements can be considerably decreased with essentially no loss in sensitivity. The volume of solution, 50 or 100 microliters, required for a single measurement is added to the nebulizer of the AAS apparatus manually with a bulb pipette, or automatically by a suitable sample changing system. A total of 500 microliters serum is sufficient for the determination of traces of Fe, Cu, and Zn. It was shown that under suitable conditions a total volume of 25 microliters serum is sufficient for the determination of Cu and Zn by flame atomic absorption-atomic emission photometry.", "contents": "[Determination of Fe, Cu and Zn by a mechanised micro-method of flame photometry (author's transl)]. By sample application in measured quantities (\"injection method\") the quality of serum required for the determination of trace elements can be considerably decreased with essentially no loss in sensitivity. The volume of solution, 50 or 100 microliters, required for a single measurement is added to the nebulizer of the AAS apparatus manually with a bulb pipette, or automatically by a suitable sample changing system. A total of 500 microliters serum is sufficient for the determination of traces of Fe, Cu, and Zn. It was shown that under suitable conditions a total volume of 25 microliters serum is sufficient for the determination of Cu and Zn by flame atomic absorption-atomic emission photometry."}
{"id": "PMID:479767", "title": "Collagen heterogeneity in systemic scleroderma and other diseases.", "content": "Proportions of collagen type I and type III were investigated in different tissues (kidney, liver, heart muscle, esophagus, lung and skin) in a single case of malignant systemic scleroderma. The proportion of collagen type III was higher than in healthy controls. Similarly, an increase in the proportion of collagen type III was shown in tissues affected by other inflammatory diseases. Further the authors suggest that the increase in total kidney collagen, especially type III, has important consequences for kidney function; in the described case it had the critical influence on the fatal course of the disease, and the patient died from uremia.", "contents": "Collagen heterogeneity in systemic scleroderma and other diseases. Proportions of collagen type I and type III were investigated in different tissues (kidney, liver, heart muscle, esophagus, lung and skin) in a single case of malignant systemic scleroderma. The proportion of collagen type III was higher than in healthy controls. Similarly, an increase in the proportion of collagen type III was shown in tissues affected by other inflammatory diseases. Further the authors suggest that the increase in total kidney collagen, especially type III, has important consequences for kidney function; in the described case it had the critical influence on the fatal course of the disease, and the patient died from uremia."}
{"id": "PMID:479772", "title": "A homestyle delivery program in a university hospital.", "content": "The Homestyle Delivery Program, an alternative birth service at the University of California Davis Medical Center, Sacramento, is presented. The program was developed jointly by the departments of family practice, obstetrics, and pediatrics, in response to the needs and desires of patients and physicians to participate in a more natural family centered birthing process. A brief description of the program and data from the first 1 1/2 years of operation is given. This program, in contrast to many other alternative birthing programs, involves physicians in training; that is, residents in family practice and obstetrics who are being taught during their obstetrical training how to create and facilitate an intimate family oriented home-like birthing. Satisfaction with the program on the part of the participating families as well as physicians and program staff has been very high. Today, more families in this society are demanding this kind of alternative birthing experience; the Homestyle Delivery Program meets their needs and to data has demonstrated no increased risk to mother or infant.", "contents": "A homestyle delivery program in a university hospital. The Homestyle Delivery Program, an alternative birth service at the University of California Davis Medical Center, Sacramento, is presented. The program was developed jointly by the departments of family practice, obstetrics, and pediatrics, in response to the needs and desires of patients and physicians to participate in a more natural family centered birthing process. A brief description of the program and data from the first 1 1/2 years of operation is given. This program, in contrast to many other alternative birthing programs, involves physicians in training; that is, residents in family practice and obstetrics who are being taught during their obstetrical training how to create and facilitate an intimate family oriented home-like birthing. Satisfaction with the program on the part of the participating families as well as physicians and program staff has been very high. Today, more families in this society are demanding this kind of alternative birthing experience; the Homestyle Delivery Program meets their needs and to data has demonstrated no increased risk to mother or infant."}
{"id": "PMID:479773", "title": "Hypertension and ischemic heart disease in a rural family practice in Israel.", "content": "Prevalence rates of hypertension and ischemic heart disease in a rural community in Israel in 1976 were 9.3% and 2.3%, respectively. The rates were related to age, sex, country of origin, education, occupation, marital status, household crowding, and smoking habits. A multiple regression analysis of all variables showed a significantly positive association between the prevalence of hypertension and age, number of children, Cochin or Moroccan origin, and divorce or widowhood. A negative association was found with agricultural work. The prevalence rate of ischemic heart disease correlated positively with age, Cochin origin, and divorce or widowhood. A stepwise linear regression analysis revealed that these variables accounted for 11.3% and 2.8% of the total variance observed, in hypertension and ischemic heart disease respectively.", "contents": "Hypertension and ischemic heart disease in a rural family practice in Israel. Prevalence rates of hypertension and ischemic heart disease in a rural community in Israel in 1976 were 9.3% and 2.3%, respectively. The rates were related to age, sex, country of origin, education, occupation, marital status, household crowding, and smoking habits. A multiple regression analysis of all variables showed a significantly positive association between the prevalence of hypertension and age, number of children, Cochin or Moroccan origin, and divorce or widowhood. A negative association was found with agricultural work. The prevalence rate of ischemic heart disease correlated positively with age, Cochin origin, and divorce or widowhood. A stepwise linear regression analysis revealed that these variables accounted for 11.3% and 2.8% of the total variance observed, in hypertension and ischemic heart disease respectively."}
{"id": "PMID:479774", "title": "The physician's duty to report the battered child syndrome.", "content": "All US jurisdictions require that physicians file formal reports of suspected cases of child abuse and/or child neglect. Most states expressly grant civil immunity from lawsuits to all reporting physicians. The physician who fails to file such a report will be exposed to civil liability for professional malpractice when failure to report leads to continued child abuse. At least 37 states provide for criminal penalties for physicians who fail to report battered children. This paper examines procedures for minimal compliance with any state's reporting statute and details the precise reporting procedure for each US jurisdiction.", "contents": "The physician's duty to report the battered child syndrome. All US jurisdictions require that physicians file formal reports of suspected cases of child abuse and/or child neglect. Most states expressly grant civil immunity from lawsuits to all reporting physicians. The physician who fails to file such a report will be exposed to civil liability for professional malpractice when failure to report leads to continued child abuse. At least 37 states provide for criminal penalties for physicians who fail to report battered children. This paper examines procedures for minimal compliance with any state's reporting statute and details the precise reporting procedure for each US jurisdiction."}
{"id": "PMID:479775", "title": "Assessing counseling skills and attitudes in family practice residents.", "content": "This is the second of two papers describing portions of the behavioral science orientation curriculum that focuses on assessing changes in counseling skills and attitudes in Charleston family practice residents. The measurement of counseling skills and attitudes is accomplished through a 38-item multiple choice instrument. The basic types of items composing the instrument are delineated. Pre and post-testing data are presented over a five-year period, 1973-1977. Three-year follow-up data are presented for the residency class entering in 1975. These data are discussed from two perspectives: (1) their function in teaching physician awareness of counseling style, and (2) their function in assessing changes in physician counseling profiles.", "contents": "Assessing counseling skills and attitudes in family practice residents. This is the second of two papers describing portions of the behavioral science orientation curriculum that focuses on assessing changes in counseling skills and attitudes in Charleston family practice residents. The measurement of counseling skills and attitudes is accomplished through a 38-item multiple choice instrument. The basic types of items composing the instrument are delineated. Pre and post-testing data are presented over a five-year period, 1973-1977. Three-year follow-up data are presented for the residency class entering in 1975. These data are discussed from two perspectives: (1) their function in teaching physician awareness of counseling style, and (2) their function in assessing changes in physician counseling profiles."}
{"id": "PMID:479776", "title": "Effectiveness of faculty development workshops in family medicine.", "content": "Family medicine programs need faculty well trained in the roles of educator, administrator, researcher, and clinician. While the need for faculty development is recognized in all colleges and departments, it is a particular problem in family medicine due to the shortage of faculty, diverse backgrounds of existing faculty, and current pressures to develop the research base for the discipline of family medicine. This study was conducted to gather information about the effectiveness of the two-to-three day workshop format for faculty development in family medicine. In a pre-post comparison and a nine-month follow-up of four faculty development workshops, significant and persistent changes were found in participants' ratings of their abilities to do faculty related skills. The three-day residential workshop was found to be an effective means for promoting faculty development.", "contents": "Effectiveness of faculty development workshops in family medicine. Family medicine programs need faculty well trained in the roles of educator, administrator, researcher, and clinician. While the need for faculty development is recognized in all colleges and departments, it is a particular problem in family medicine due to the shortage of faculty, diverse backgrounds of existing faculty, and current pressures to develop the research base for the discipline of family medicine. This study was conducted to gather information about the effectiveness of the two-to-three day workshop format for faculty development in family medicine. In a pre-post comparison and a nine-month follow-up of four faculty development workshops, significant and persistent changes were found in participants' ratings of their abilities to do faculty related skills. The three-day residential workshop was found to be an effective means for promoting faculty development."}
{"id": "PMID:479777", "title": "Three approaches to problem solving in medicine and some problems they create.", "content": "Three general approaches to problem solving in medicine are examined: Basic Science Problem Solving, Clinical Medicine Problem Solving, and Human Values Problem Solving. These three approaches employ different--indeed contradictory--search strategies. Not infrequently, students of medicine become versed in one or another problem solving approach only to flounder when they try to apply it inappropriately. Recognition of this problem may help to solve it.", "contents": "Three approaches to problem solving in medicine and some problems they create. Three general approaches to problem solving in medicine are examined: Basic Science Problem Solving, Clinical Medicine Problem Solving, and Human Values Problem Solving. These three approaches employ different--indeed contradictory--search strategies. Not infrequently, students of medicine become versed in one or another problem solving approach only to flounder when they try to apply it inappropriately. Recognition of this problem may help to solve it."}
{"id": "PMID:479778", "title": "The effect of relocation of a family practice center on one resident's experience in continuity of care.", "content": "In July 1977, the Hollywood Family Practice Unit of the University of Miami Department of Family Medicine was relocated eight miles from its former structure. Analysis of patient encounter data gathered by one resident during the 2 six-month periods prior to and following the move showed little effect of the relocation on five indexes of continuity of care. According to four indexes (percentage of revisits, percentage of revisits for new problems, percentage of revisits for previously recorded problems, and percentage of families with more than one member attending), the author experienced a gradual and uninterrupted increase in continuity of patient care. A fith index, percentage of psychosocial diagnoses, dropped slightly during the third study period. Overall continuity was low, however, when compared with established practices. Although family practice residencies should accept disruption in continuity of patient care due to their structure and purposes, steps are noted which can be taken to improve continuity of care.", "contents": "The effect of relocation of a family practice center on one resident's experience in continuity of care. In July 1977, the Hollywood Family Practice Unit of the University of Miami Department of Family Medicine was relocated eight miles from its former structure. Analysis of patient encounter data gathered by one resident during the 2 six-month periods prior to and following the move showed little effect of the relocation on five indexes of continuity of care. According to four indexes (percentage of revisits, percentage of revisits for new problems, percentage of revisits for previously recorded problems, and percentage of families with more than one member attending), the author experienced a gradual and uninterrupted increase in continuity of patient care. A fith index, percentage of psychosocial diagnoses, dropped slightly during the third study period. Overall continuity was low, however, when compared with established practices. Although family practice residencies should accept disruption in continuity of patient care due to their structure and purposes, steps are noted which can be taken to improve continuity of care."}
{"id": "PMID:479779", "title": "Maximal exercise testing--Bruce protocol.", "content": "Maximal treadmill exercise testing is feasible for the family physician's office especially in a group setting. The Bruce Exercise Test is described in detail and is helpful in the diagnosis of asymptomatic coronary heart disease, following the course of coronary heart disease, diagnosis of other cardiac disease, and in preventive testing and exercise prescription. Sensitivity and specificity of ST changes may be improved by recognizing factors which cause false positive and negative ST responses. The Bruce Test is safe when the procedure is followed meticulously. There have been 2,490 tests performed in the laboratory of three family physicians without serious morbidity. The screening exercise test with preventive exercise prescription is particularly pertinent to the present day concepts of family practice.", "contents": "Maximal exercise testing--Bruce protocol. Maximal treadmill exercise testing is feasible for the family physician's office especially in a group setting. The Bruce Exercise Test is described in detail and is helpful in the diagnosis of asymptomatic coronary heart disease, following the course of coronary heart disease, diagnosis of other cardiac disease, and in preventive testing and exercise prescription. Sensitivity and specificity of ST changes may be improved by recognizing factors which cause false positive and negative ST responses. The Bruce Test is safe when the procedure is followed meticulously. There have been 2,490 tests performed in the laboratory of three family physicians without serious morbidity. The screening exercise test with preventive exercise prescription is particularly pertinent to the present day concepts of family practice."}
{"id": "PMID:479785", "title": "Rubella seroconversion following immunization in a rural practice.", "content": "One hundred two consecutive patients with verifiable immunization to rubella were studied in a rural West Virginia county. Hemagglutination Inhibiting (HI) antibody titres, age at immunization, and elapsed time since immunization were recorded. Twenty-three (22.5 percent) had HI titres less than 1:8. There was no statistically significant difference in whether immunization at less than 15 months or more than 15 months of age resulted in a titre of greater than or equal to 1:8. There was no evident change in group proportion of seroconversion with time; either the child initially had a seroconversion with immunization, or he/she did not. A relatively large number of children with verified immunization to rubella do not have a titre currently considered protective to rubella. This does not seem to vary with time since immunization or age at immunization.", "contents": "Rubella seroconversion following immunization in a rural practice. One hundred two consecutive patients with verifiable immunization to rubella were studied in a rural West Virginia county. Hemagglutination Inhibiting (HI) antibody titres, age at immunization, and elapsed time since immunization were recorded. Twenty-three (22.5 percent) had HI titres less than 1:8. There was no statistically significant difference in whether immunization at less than 15 months or more than 15 months of age resulted in a titre of greater than or equal to 1:8. There was no evident change in group proportion of seroconversion with time; either the child initially had a seroconversion with immunization, or he/she did not. A relatively large number of children with verified immunization to rubella do not have a titre currently considered protective to rubella. This does not seem to vary with time since immunization or age at immunization."}
{"id": "PMID:479786", "title": "Post-abortion attitudes and patterns of birth control.", "content": "Among 63 women questioned one year after a first trimester abortion at an outpatient clinic, over 75 percent had mature, well-considered reasons for having chosen abortion and felt secure in their decision. Their feelings and attitudes toward themselves and abortion were strongly positive, with a few expressions of unresolved guilt, regret, or depression. They had become more responsible about contraceptive use, more interested in planning future pregnancies, and had experienced little change in sexual habits. Although only 21 percent (13 women) had employed contraception when they became pregnant, 76 percent (48 women) were using effective methods of birth control one year after abortion. Furthermore, over one third felt they were better able to deal with problems and had learned more about their coping capacity as a result of the crisis.", "contents": "Post-abortion attitudes and patterns of birth control. Among 63 women questioned one year after a first trimester abortion at an outpatient clinic, over 75 percent had mature, well-considered reasons for having chosen abortion and felt secure in their decision. Their feelings and attitudes toward themselves and abortion were strongly positive, with a few expressions of unresolved guilt, regret, or depression. They had become more responsible about contraceptive use, more interested in planning future pregnancies, and had experienced little change in sexual habits. Although only 21 percent (13 women) had employed contraception when they became pregnant, 76 percent (48 women) were using effective methods of birth control one year after abortion. Furthermore, over one third felt they were better able to deal with problems and had learned more about their coping capacity as a result of the crisis."}
{"id": "PMID:479787", "title": "Comparative content of three family practice residency programs.", "content": "A method of collecting physician-patient encounter data is described and applied to the comparison of the clinical content of residents' experiences in the family practice office. Comparisons were made of over 1,000 consecutive unselected encounters in each of the three family practice residency programs. Considerable differences between the patients encountered by the three programs were observed and between program patients and patients attending family physicians in Iowa. It is important to monitor the demography and diagnostic status of patients attending family practice residency programs to ensure the authenticity of the residents' experience.", "contents": "Comparative content of three family practice residency programs. A method of collecting physician-patient encounter data is described and applied to the comparison of the clinical content of residents' experiences in the family practice office. Comparisons were made of over 1,000 consecutive unselected encounters in each of the three family practice residency programs. Considerable differences between the patients encountered by the three programs were observed and between program patients and patients attending family physicians in Iowa. It is important to monitor the demography and diagnostic status of patients attending family practice residency programs to ensure the authenticity of the residents' experience."}
{"id": "PMID:479788", "title": "Recognition of depression in a university-based family medicine residency program.", "content": "Physicians diagnosed depression in 4.5 percent (59/1,321) of all patient encounters in this study conducted in a university based family medicine residency program. A subset of residents permitted the investigators to interview those patients who were diagnosed as depressed or who were judged to be at high risk for a missed diagnosis of depression. Of the six patients whom the residents diagnosed as depressed and who agreed to be interviewed, two did not meet the criteria for depression but in neither case was this discrepancy judged clinically significant. Of the 24 patients at high risk for a missed diagnosis who agreed to be interviewed, four met the diagnostic criteria and three of these four missed diagnoses were judged as possibly of clinical significance. While the physicians in this study diagnosed depression more frequently than in previous similar studies, this does not appear to represent over-diagnosis, although the small number of patients interviewed requires that interpretations be made cautiously. The authors suggest that if a primary care physician diagnoses depression in less than 2 percent of patient encounters, he or she should consider the possibility of missed diagnoses.", "contents": "Recognition of depression in a university-based family medicine residency program. Physicians diagnosed depression in 4.5 percent (59/1,321) of all patient encounters in this study conducted in a university based family medicine residency program. A subset of residents permitted the investigators to interview those patients who were diagnosed as depressed or who were judged to be at high risk for a missed diagnosis of depression. Of the six patients whom the residents diagnosed as depressed and who agreed to be interviewed, two did not meet the criteria for depression but in neither case was this discrepancy judged clinically significant. Of the 24 patients at high risk for a missed diagnosis who agreed to be interviewed, four met the diagnostic criteria and three of these four missed diagnoses were judged as possibly of clinical significance. While the physicians in this study diagnosed depression more frequently than in previous similar studies, this does not appear to represent over-diagnosis, although the small number of patients interviewed requires that interpretations be made cautiously. The authors suggest that if a primary care physician diagnoses depression in less than 2 percent of patient encounters, he or she should consider the possibility of missed diagnoses."}
{"id": "PMID:479790", "title": "Group process in teaching family dynamics to family practice residents.", "content": "Behavioral science training in family practice at Madigan Army Medical Center, Tacoma, Washington, has used small group seminars to teach family dynamics on an experiential level. The group process is similar enough to dynamics within families to facilitate understanding by introspection. Small groups are an efficient method for teaching, and allow experiential learning to occur spontaneously as well as on a planned basis.", "contents": "Group process in teaching family dynamics to family practice residents. Behavioral science training in family practice at Madigan Army Medical Center, Tacoma, Washington, has used small group seminars to teach family dynamics on an experiential level. The group process is similar enough to dynamics within families to facilitate understanding by introspection. Small groups are an efficient method for teaching, and allow experiential learning to occur spontaneously as well as on a planned basis."}
{"id": "PMID:479791", "title": "Clinical experience of medical students with primary care career goals.", "content": "Two medical students with primary care career goals, in a medical school based at a university medical center, recorded demographic and medical problem information from their patients. They saw a total of 3,391 problems in 2,265 different patients, resulting in a clinical experience similar to that of students from a medical school not based at a university medical center and residents in two family medicine residency training sites. Almost 62.0 percent of problem contacts were seen outside of the medical center hospital, 66.7 percent on ambulatory patients, and 47.3 percent on non-credit rotations, reflecting the intensity of their personal motivation, the support of faculty with primary care backgrounds, and the flexibility of a clinical curriculum offering 50 percent electives. The study demonstrates that it is possible for students in a university medical center to obtain clinical experience with patient problems of the type encountered in the community.", "contents": "Clinical experience of medical students with primary care career goals. Two medical students with primary care career goals, in a medical school based at a university medical center, recorded demographic and medical problem information from their patients. They saw a total of 3,391 problems in 2,265 different patients, resulting in a clinical experience similar to that of students from a medical school not based at a university medical center and residents in two family medicine residency training sites. Almost 62.0 percent of problem contacts were seen outside of the medical center hospital, 66.7 percent on ambulatory patients, and 47.3 percent on non-credit rotations, reflecting the intensity of their personal motivation, the support of faculty with primary care backgrounds, and the flexibility of a clinical curriculum offering 50 percent electives. The study demonstrates that it is possible for students in a university medical center to obtain clinical experience with patient problems of the type encountered in the community."}
{"id": "PMID:479792", "title": "Teaching physicians to teach: a three-year report.", "content": "To meet the needs for an expanded preceptor faculty, the Department of Family Medicine at the College of Medicine and Dentistry of New Jersey-Rutgers Medical School has for three years conducted yearly training programs designed to prepare practicing family physicians for the teaching role. Thirty-six physicians have completed the program, which consists of four group seminars and three individual learning site visits spent in the office of an experienced preceptor while a fourth year student is present. Many lessons were learned in the course of these yearly programs which may be useful to others who plan to undertake similar faculty development activities. Therefore, detailed, practical, experiential information is presented regarding recruitment, orientation, the educational program of seminars and individual learning experiences, evaluation, and required resources. Some problems proved to be particularly difficult, such as the uneven quality of the individual learning visits and the attrition of some participants from the program. Feedback from participating physicians has been extremely positive.", "contents": "Teaching physicians to teach: a three-year report. To meet the needs for an expanded preceptor faculty, the Department of Family Medicine at the College of Medicine and Dentistry of New Jersey-Rutgers Medical School has for three years conducted yearly training programs designed to prepare practicing family physicians for the teaching role. Thirty-six physicians have completed the program, which consists of four group seminars and three individual learning site visits spent in the office of an experienced preceptor while a fourth year student is present. Many lessons were learned in the course of these yearly programs which may be useful to others who plan to undertake similar faculty development activities. Therefore, detailed, practical, experiential information is presented regarding recruitment, orientation, the educational program of seminars and individual learning experiences, evaluation, and required resources. Some problems proved to be particularly difficult, such as the uneven quality of the individual learning visits and the attrition of some participants from the program. Feedback from participating physicians has been extremely positive."}
{"id": "PMID:479793", "title": "Concerns and challenges facing social workers in family practice.", "content": "Twenty-five social work members of the Society of Teachers of Family Medicine responded to a survey in which they identified their chief concerns and challenges encountered in family practice. Of nine areas of concern to social workers in interdisciplinary practice identified from the literature, respondents viewed as most important the achievement of acceptance and regard among other disciplines for the social worker and the value of his/her contribution to family practice. This was followed closely by the associated challenges of developing interdisciplinary relationships and fostering an accurate understanding of the role functions of the social worker in family practice. To confront these and other concerns, careful attention to three major needs suggested by the survey results is advocated: (1) the need for social workers to maintain high standards of performance in family practice, (2) the need for family physicians and other health care personnel to understand the role functions of the social worker and examine their attitudes toward collaboration with social workers, and (3) the need for regular and constructive dialogue between all personnel in family practice.", "contents": "Concerns and challenges facing social workers in family practice. Twenty-five social work members of the Society of Teachers of Family Medicine responded to a survey in which they identified their chief concerns and challenges encountered in family practice. Of nine areas of concern to social workers in interdisciplinary practice identified from the literature, respondents viewed as most important the achievement of acceptance and regard among other disciplines for the social worker and the value of his/her contribution to family practice. This was followed closely by the associated challenges of developing interdisciplinary relationships and fostering an accurate understanding of the role functions of the social worker in family practice. To confront these and other concerns, careful attention to three major needs suggested by the survey results is advocated: (1) the need for social workers to maintain high standards of performance in family practice, (2) the need for family physicians and other health care personnel to understand the role functions of the social worker and examine their attitudes toward collaboration with social workers, and (3) the need for regular and constructive dialogue between all personnel in family practice."}
{"id": "PMID:479794", "title": "Specialty preference and attitudes toward the aged.", "content": "The relationship between the attitudes and knowledge regarding the aged and specialty preference of undergraduate medical students at three medical schools was studied. No significant differences in attitudes were found when students were classified by class (freshman, sophomore, junior, or senior) or by sex. A significant though weak relationship between class and level of cognitive knowledge was found. Students indicating a preference for family practice as a specialty demonstrated levels of cognitive knowledge which did not differ significantly from all other students. Similarly, attitudes of family practice students did not differ significantly from attitudes expressed by other students. However, when all students expressing a preference for a primary care specialty were compared to students expressing preferences for non-primary care specialties, primary care students had significantly more positive attitudes toward the aged than their peers. Levels of cognitive knowledge did not differ significantly. The data appeared to suggest that students selecting primary care specialties are somewhat more humanistic and empathetically oriented, at least in regard to the aged, than their peers while exhibiting essentially identical levels of cognitive knowledge. The implications of these findings for family practice education are discussed.", "contents": "Specialty preference and attitudes toward the aged. The relationship between the attitudes and knowledge regarding the aged and specialty preference of undergraduate medical students at three medical schools was studied. No significant differences in attitudes were found when students were classified by class (freshman, sophomore, junior, or senior) or by sex. A significant though weak relationship between class and level of cognitive knowledge was found. Students indicating a preference for family practice as a specialty demonstrated levels of cognitive knowledge which did not differ significantly from all other students. Similarly, attitudes of family practice students did not differ significantly from attitudes expressed by other students. However, when all students expressing a preference for a primary care specialty were compared to students expressing preferences for non-primary care specialties, primary care students had significantly more positive attitudes toward the aged than their peers. Levels of cognitive knowledge did not differ significantly. The data appeared to suggest that students selecting primary care specialties are somewhat more humanistic and empathetically oriented, at least in regard to the aged, than their peers while exhibiting essentially identical levels of cognitive knowledge. The implications of these findings for family practice education are discussed."}
{"id": "PMID:479795", "title": "Referral patterns of family physicians in an underserved rural area.", "content": "This paper examines the rates and patterns of referral of four family physicians in an underserved rural area of Washington State. The majority of referrals made by the family physicians were for management of a particular problem outside the scope of practice of the physician. The referral rates ranged from 1.8 to 3.2 percent of all patient visits and were comparable with those previously reported. A National Health Service Corps physician had the highest rate of referral and a foreign medical graduate had the lowest rate of referral. These results may be explained by practice building incentives as well as the ability to establish ties with the referral network.", "contents": "Referral patterns of family physicians in an underserved rural area. This paper examines the rates and patterns of referral of four family physicians in an underserved rural area of Washington State. The majority of referrals made by the family physicians were for management of a particular problem outside the scope of practice of the physician. The referral rates ranged from 1.8 to 3.2 percent of all patient visits and were comparable with those previously reported. A National Health Service Corps physician had the highest rate of referral and a foreign medical graduate had the lowest rate of referral. These results may be explained by practice building incentives as well as the ability to establish ties with the referral network."}
{"id": "PMID:479810", "title": "The oscillatory responses of skate electroreceptors to small voltage stimuli.", "content": "Tonic nerve activity in skate electroreceptors is thought to result from spontaneous activity of the lumenal membranes of the receptor cells which is modulated by applied stimuli. When physiological conditions are simulated in vitro, the receptor epithelium produces a current which flows inward across the lumenal surface. This epithelial current exhibits small spontaneous sinusoidal fluctuations about the mean that are associated with corresponding but delayed fluctuations in postsynaptic response. Small voltage stimuli produce damped oscillations in the epithelial current similar in time-course to the spontaneous fluctuations. For lumen-negative, excitatory stimuli, these responses are predominantly an increase over the mean inward current. For inhibitory stimuli they are predominantly a decrease. Increased inward current across the lumenal membranes of the receptor cells increases depolarization of the presynaptic membranes in the basal faces leading to increased release of transmitter and an excitatory postsynaptic response. Decreased inward current decreases depolarization of the presynaptic membranes leading to a reduction in transmitter release and an inhibitory postsynaptic response. Clear changes in postsynaptic response are detectable during stimuli as small as 5 microV with saturation occurring at +/- 400 microV. The evoked oscillations in epithelial current are damped and the postsynaptic responses decline during maintained stimuli with large off-responses occurring at stimulus termination. The initial peak of the off-response is similar to the response produced by onset of an oppositely directed stimulus. These observations substantiate the role of receptor cell excitability in the detection of small voltage changes.", "contents": "The oscillatory responses of skate electroreceptors to small voltage stimuli. Tonic nerve activity in skate electroreceptors is thought to result from spontaneous activity of the lumenal membranes of the receptor cells which is modulated by applied stimuli. When physiological conditions are simulated in vitro, the receptor epithelium produces a current which flows inward across the lumenal surface. This epithelial current exhibits small spontaneous sinusoidal fluctuations about the mean that are associated with corresponding but delayed fluctuations in postsynaptic response. Small voltage stimuli produce damped oscillations in the epithelial current similar in time-course to the spontaneous fluctuations. For lumen-negative, excitatory stimuli, these responses are predominantly an increase over the mean inward current. For inhibitory stimuli they are predominantly a decrease. Increased inward current across the lumenal membranes of the receptor cells increases depolarization of the presynaptic membranes in the basal faces leading to increased release of transmitter and an excitatory postsynaptic response. Decreased inward current decreases depolarization of the presynaptic membranes leading to a reduction in transmitter release and an inhibitory postsynaptic response. Clear changes in postsynaptic response are detectable during stimuli as small as 5 microV with saturation occurring at +/- 400 microV. The evoked oscillations in epithelial current are damped and the postsynaptic responses decline during maintained stimuli with large off-responses occurring at stimulus termination. The initial peak of the off-response is similar to the response produced by onset of an oppositely directed stimulus. These observations substantiate the role of receptor cell excitability in the detection of small voltage changes."}
{"id": "PMID:479811", "title": "The ionic basis of oscillatory responses of skate electroreceptors.", "content": "When physiological conditions are simulated, skate electroreceptors produce small maintained oscillatory currents. Larger damped oscillations of similar time-course are observed in voltage clamp. Subtraction of leakage in voltage clamp data shows that the oscillations involve no net outward current across the lumenal surface of the epithelium. The oscillations are much faster than the late outward current generated by the lumenal membranes of the receptor cells. Treatment of the basal surface of the epithelium with tetraethyl ammonium (TEA), high K, Co, or EGTA reversibly blocks the oscillations in voltage clamp, but has little or no effect on the epithelial action potential in current clamp or on the current-voltage relation. The TEA sensitivity of the oscillations indicates that they involve a potassium conductance in the basal membranes of the receptor cells. Treatment of the basal membranes with TEA and high calcium, with strontium, or with barium causes these membranes to produce large regenerative responses. Direct stimulation of the basal membranes then elicits a lumen-positive action potential whereas stimulation of the lumenal membranes elicits a diphasic action potential. Excitability of the basal membranes is abolished by extracellular Co, Mn, or La. Modulation of the lumenal membrane calcium conductance by the basal membrane conductances probably gives rise to the oscillatory receptor currents evoked by small voltage stimuli. The slower calcium-activated late conductance in the lumenal membranes may be involved in sensory accommodation.", "contents": "The ionic basis of oscillatory responses of skate electroreceptors. When physiological conditions are simulated, skate electroreceptors produce small maintained oscillatory currents. Larger damped oscillations of similar time-course are observed in voltage clamp. Subtraction of leakage in voltage clamp data shows that the oscillations involve no net outward current across the lumenal surface of the epithelium. The oscillations are much faster than the late outward current generated by the lumenal membranes of the receptor cells. Treatment of the basal surface of the epithelium with tetraethyl ammonium (TEA), high K, Co, or EGTA reversibly blocks the oscillations in voltage clamp, but has little or no effect on the epithelial action potential in current clamp or on the current-voltage relation. The TEA sensitivity of the oscillations indicates that they involve a potassium conductance in the basal membranes of the receptor cells. Treatment of the basal membranes with TEA and high calcium, with strontium, or with barium causes these membranes to produce large regenerative responses. Direct stimulation of the basal membranes then elicits a lumen-positive action potential whereas stimulation of the lumenal membranes elicits a diphasic action potential. Excitability of the basal membranes is abolished by extracellular Co, Mn, or La. Modulation of the lumenal membrane calcium conductance by the basal membrane conductances probably gives rise to the oscillatory receptor currents evoked by small voltage stimuli. The slower calcium-activated late conductance in the lumenal membranes may be involved in sensory accommodation."}
{"id": "PMID:479812", "title": "Nonlinear cable equations for axons. I. Computations and experiments with internal current injection.", "content": "Steady-state potential and current distributions resulting from internal injection of current in the squid giant axon have been measured experimentally and also computed from nonlinear membrane cable equation models by numerical methods, using the Hodgkin-Huxley equations to give the membrane current density. The solutions obtained by this method satisfactorily reproduce experimental measurements of the steady-state distribution of membrane potential. Computations of the input current-voltage characteristic for a nonlinear cable were in excellent agreement with measurements on axons. Our results demonstrate the power of Cole's equation to extract the nonlinear membrane characteristics simply from measurement of the input resistance.", "contents": "Nonlinear cable equations for axons. I. Computations and experiments with internal current injection. Steady-state potential and current distributions resulting from internal injection of current in the squid giant axon have been measured experimentally and also computed from nonlinear membrane cable equation models by numerical methods, using the Hodgkin-Huxley equations to give the membrane current density. The solutions obtained by this method satisfactorily reproduce experimental measurements of the steady-state distribution of membrane potential. Computations of the input current-voltage characteristic for a nonlinear cable were in excellent agreement with measurements on axons. Our results demonstrate the power of Cole's equation to extract the nonlinear membrane characteristics simply from measurement of the input resistance."}
{"id": "PMID:479813", "title": "Nonlinear cable equations for axons. II. Computations and experiments with external current electrodes.", "content": "We have investigated the steady-state potential and current distributions resulting from current injection into a close-fitting channel into which a squid axon is placed. Hybrid computer solutions of the cable equations, using the Hodgkin-Huxley equations to give the membrane current density, were in good agreement with experimental observations. A much better fit was obtained when the Hodgkin-Huxley leakage conductance was reduced fivefold.", "contents": "Nonlinear cable equations for axons. II. Computations and experiments with external current electrodes. We have investigated the steady-state potential and current distributions resulting from current injection into a close-fitting channel into which a squid axon is placed. Hybrid computer solutions of the cable equations, using the Hodgkin-Huxley equations to give the membrane current density, were in good agreement with experimental observations. A much better fit was obtained when the Hodgkin-Huxley leakage conductance was reduced fivefold."}
{"id": "PMID:479814", "title": "Organotin-mediated exchange diffusion of anions in human red cells.", "content": "Organotin cations (R3Sn+) form electrically neutral ion pairs with monovalent anions. It is demonstrated that the tin derivatives induce exchange diffusion of chloride in red cells and resealed ghosts, without any detectable increase of membrane permeability to net movements of chloride ions. The obligatory anion exchange is believed to be due to the permeation of electroneural ion pairs, whereas the organic cation (R3Sn+) has an extremely low membrane permeability. Exchange fluxes of chloride increased with the lipophilicity of the substituting group (R3). At the same molar concentration of organotin, the relative potencies of the tin derivatives as anion carriers (with trimethyltin as a reference) were: methyl 1, ethyl 30, propyl = phenyl 1,00, and butyl 10,000. Tributyltin-mediated anion exchange was studied in detail. The organotin-induced anion transport increased through the sequence: F- less than Cl- less than Br- less than I- = SCN- less than OH-. Partitioning of tributyltin into red cell membranes was greater in iodide than in chloride media (partition coefficients 6.6 and 1.7 x 10(-3) cm, respectively). Bicarbonate, fluoride, nitrate, phosphate, and sulphate did not exchange with chloride in the presence of tributyltin. Chloride exchange fluxes increased linearly with tributylin concentrations up to 10(-5) M, and with chloride concentrations up to at least 0.9 M. The apparent turnover number for tributyltin-mediated chloride exchange increased from 15 to 1,350 s-1 between 0 and 38 degrees C. These figures are minimum turnover numbers, because it is not known what fraction of the organotin in the membrane exists as chloride ion pairs.", "contents": "Organotin-mediated exchange diffusion of anions in human red cells. Organotin cations (R3Sn+) form electrically neutral ion pairs with monovalent anions. It is demonstrated that the tin derivatives induce exchange diffusion of chloride in red cells and resealed ghosts, without any detectable increase of membrane permeability to net movements of chloride ions. The obligatory anion exchange is believed to be due to the permeation of electroneural ion pairs, whereas the organic cation (R3Sn+) has an extremely low membrane permeability. Exchange fluxes of chloride increased with the lipophilicity of the substituting group (R3). At the same molar concentration of organotin, the relative potencies of the tin derivatives as anion carriers (with trimethyltin as a reference) were: methyl 1, ethyl 30, propyl = phenyl 1,00, and butyl 10,000. Tributyltin-mediated anion exchange was studied in detail. The organotin-induced anion transport increased through the sequence: F- less than Cl- less than Br- less than I- = SCN- less than OH-. Partitioning of tributyltin into red cell membranes was greater in iodide than in chloride media (partition coefficients 6.6 and 1.7 x 10(-3) cm, respectively). Bicarbonate, fluoride, nitrate, phosphate, and sulphate did not exchange with chloride in the presence of tributyltin. Chloride exchange fluxes increased linearly with tributylin concentrations up to 10(-5) M, and with chloride concentrations up to at least 0.9 M. The apparent turnover number for tributyltin-mediated chloride exchange increased from 15 to 1,350 s-1 between 0 and 38 degrees C. These figures are minimum turnover numbers, because it is not known what fraction of the organotin in the membrane exists as chloride ion pairs."}
{"id": "PMID:479815", "title": "Tributyltin-mediated exchange diffusion of halides in lipid bilayers.", "content": "This paper describes the effect of tributyltin (TBT) on the inorganic anion permeability of lipid bilayers. When this compound is added in micromolar concentrations to one or both sides of a phosphatidyl ethanolamine (PE) membrane formed in 0.1 M NaCl or KCl (pH 7), there is no change in the electrical conductance. Under these circumstances, the Cl self-exchange flux measured with 36Cl (MCl) increases from a value of approximately 10(-12) mol.cm-2.s-1, to approximately 10(-8) mol.cm-2.s-1. It was further found that the relation between chloride flux and [TBT] and [Cl] can be described as: MCl = B[TBT] [Cl]. When chloride was replaced by an equimolar concentration of different univalent anions in the trans compartment, the heteroexchange flux of chloride followed the sequence: I greater than Br greater than Cl greater than F greater than NO3. Under all experimental conditions tested, the chloride flux was always more than 10(3) times the maximum flux predicted from the value of the membrane conductance, and at least 100 times higher than the expected fluxes of ion pairs (TBT-Cl) diffusing across the unstirred layers. Thus, the mechanism by which tributyltin increases anion permeability in bilayers seems to be that of an obligatory exchange diffusion, with the reaction between tributyltin and the halides occurring at the membrane surface. Measurements of interfacial potentials indicate that tributyltin chloride lowers the positive intrinsic dipole potential of PE membranes by approximately 70 mV (at a TBT concentration of 30 microM) without substantial alteration of other parameters of the bilayer. The estimated adsorption coefficient of TBT-Cl was found to be 3 x 10(-4) cm.", "contents": "Tributyltin-mediated exchange diffusion of halides in lipid bilayers. This paper describes the effect of tributyltin (TBT) on the inorganic anion permeability of lipid bilayers. When this compound is added in micromolar concentrations to one or both sides of a phosphatidyl ethanolamine (PE) membrane formed in 0.1 M NaCl or KCl (pH 7), there is no change in the electrical conductance. Under these circumstances, the Cl self-exchange flux measured with 36Cl (MCl) increases from a value of approximately 10(-12) mol.cm-2.s-1, to approximately 10(-8) mol.cm-2.s-1. It was further found that the relation between chloride flux and [TBT] and [Cl] can be described as: MCl = B[TBT] [Cl]. When chloride was replaced by an equimolar concentration of different univalent anions in the trans compartment, the heteroexchange flux of chloride followed the sequence: I greater than Br greater than Cl greater than F greater than NO3. Under all experimental conditions tested, the chloride flux was always more than 10(3) times the maximum flux predicted from the value of the membrane conductance, and at least 100 times higher than the expected fluxes of ion pairs (TBT-Cl) diffusing across the unstirred layers. Thus, the mechanism by which tributyltin increases anion permeability in bilayers seems to be that of an obligatory exchange diffusion, with the reaction between tributyltin and the halides occurring at the membrane surface. Measurements of interfacial potentials indicate that tributyltin chloride lowers the positive intrinsic dipole potential of PE membranes by approximately 70 mV (at a TBT concentration of 30 microM) without substantial alteration of other parameters of the bilayer. The estimated adsorption coefficient of TBT-Cl was found to be 3 x 10(-4) cm."}
{"id": "PMID:479816", "title": "Chloride and sodium influx: a coupled uptake mechanism in the squid giant axon.", "content": "The squid giant axon was internally dialyzed while the unidirectional fluxes of either Cl or Na were measured. The effects of varying the internal or external concentration of either Na or Cl were studied. Chloride influx was directly proportional to the external Na concentration whereas Cl efflux was unaffected by changes of the external Na concentration between 0 and 425 mM. Neither Cl influx nor efflux were affected by changes of internal Na concentration over the range of 8-158 mM. After ouabain and TTX treatment a portion of the remaining Na influx was directly dependent on the extracellular Cl concentration. Furthermore, when the internal Cl concentration was increased from 0 to 150 mM, the influxes of Cl and Na were decreased by 14 and 11 pmol/cm2.s, respectively. The influx of both ions could be substantially reduced when the axon was depleted of ATP. The influxes of both ions were inhibited by furosemide but unaffected by ouabain. It is concluded that the squid axolemma has an ATP-dependent coupled Na-Cl co-transport uptake mechanism.", "contents": "Chloride and sodium influx: a coupled uptake mechanism in the squid giant axon. The squid giant axon was internally dialyzed while the unidirectional fluxes of either Cl or Na were measured. The effects of varying the internal or external concentration of either Na or Cl were studied. Chloride influx was directly proportional to the external Na concentration whereas Cl efflux was unaffected by changes of the external Na concentration between 0 and 425 mM. Neither Cl influx nor efflux were affected by changes of internal Na concentration over the range of 8-158 mM. After ouabain and TTX treatment a portion of the remaining Na influx was directly dependent on the extracellular Cl concentration. Furthermore, when the internal Cl concentration was increased from 0 to 150 mM, the influxes of Cl and Na were decreased by 14 and 11 pmol/cm2.s, respectively. The influx of both ions could be substantially reduced when the axon was depleted of ATP. The influxes of both ions were inhibited by furosemide but unaffected by ouabain. It is concluded that the squid axolemma has an ATP-dependent coupled Na-Cl co-transport uptake mechanism."}
{"id": "PMID:479817", "title": "Electrogenic sodium extrusion in cardiac Purkinje fibers.", "content": "Thin canine cardiac Purkinje fibers in a fast flow chamber were exposed to K-free fluid for 15 s to 6 min to initiate \"sodium loading,\" then returned to K-containing fluid to stimulate the sodium pump. The electrophysiological effects of enhanced pump activity may result from extracellular K depletion caused by enhanced cellular uptake of K or from an increase in the current generated as a result of unequal pumped movements of Na and K, or from both. The effects of pump stimulation were therefore studied under three conditions in which lowering the external K concentration ([K]0) causes changes opposite to those expected from an increase in pump current. First, the resting potential of Purkinje fibers may have either a \"high\" value of a \"low\" (less negative) value: at the low level of potential, experimental reduction of [K]0 causes depolarization, whereas an increase in pump current should cause hyperpolarization. Second, in regularly stimulated Purkinje fibers, lowering [K]0 prolongs the action potential, whereas an increase in outward pump current should shorten it. Finally, lowering [K]0 enhances spontaneous \"pacemaker\" activity in Purkinje fibers, whereas an increase in outward pump current should reduce or abolish spontaneous activity. Under all three conditions, we find that the effects of temporary stimulation of the sodium pump are those expected from a transient increase in outward pump current, not those expected from K depletion.", "contents": "Electrogenic sodium extrusion in cardiac Purkinje fibers. Thin canine cardiac Purkinje fibers in a fast flow chamber were exposed to K-free fluid for 15 s to 6 min to initiate \"sodium loading,\" then returned to K-containing fluid to stimulate the sodium pump. The electrophysiological effects of enhanced pump activity may result from extracellular K depletion caused by enhanced cellular uptake of K or from an increase in the current generated as a result of unequal pumped movements of Na and K, or from both. The effects of pump stimulation were therefore studied under three conditions in which lowering the external K concentration ([K]0) causes changes opposite to those expected from an increase in pump current. First, the resting potential of Purkinje fibers may have either a \"high\" value of a \"low\" (less negative) value: at the low level of potential, experimental reduction of [K]0 causes depolarization, whereas an increase in pump current should cause hyperpolarization. Second, in regularly stimulated Purkinje fibers, lowering [K]0 prolongs the action potential, whereas an increase in outward pump current should shorten it. Finally, lowering [K]0 enhances spontaneous \"pacemaker\" activity in Purkinje fibers, whereas an increase in outward pump current should reduce or abolish spontaneous activity. Under all three conditions, we find that the effects of temporary stimulation of the sodium pump are those expected from a transient increase in outward pump current, not those expected from K depletion."}
{"id": "PMID:479818", "title": "Structural changes in single muscle fibers after stimulation at a low frequency.", "content": "Direct stimulation of single muscle fibers from Xenopus laevis at a frequency of 1 Hz results in a decline of the peak isometric twitch tension after about 200 twitches. Fibers were chemically fixed in glutaraldehyde after a varying number of twitches and at several fatigue levels, and the ultrastructural appearance was compared with that of resting fibers treated by identical fixation methods. No gross structural abnormalities were observed but subtle changes occurred. The mitochondria of stimulated fibers contain granules of normal size and number. The inner crista width is constant but the matrix width is increased on stimulation. These changes would not compromise ATP production. The myofibrils are normal except for a slight swelling in the myosin lattice. The transverse system (T system) and sarcoplasmic reticulum are intact. The minor diameter of the transverse tubule (T tubule) is increased slightly in stimulated fibers. The gap between the T-TC membranes stays constant at about 110 A, but tiny connecting pillars are seen to cross this gap more frequently in stimulated fibers (21 +/- 5% triads) than in resting fibers (8 +/- 6%). In stimulated fibers there is a marked increase in the electron dense content of the terminal cisternae (TC). Inasmuch as the observed structural changes correlate with the number of twitches but not with the fatigue level, it is concluded that TC density and T-TC pillar formation are related to the normal mechanisms of excitation-contraction coupling.", "contents": "Structural changes in single muscle fibers after stimulation at a low frequency. Direct stimulation of single muscle fibers from Xenopus laevis at a frequency of 1 Hz results in a decline of the peak isometric twitch tension after about 200 twitches. Fibers were chemically fixed in glutaraldehyde after a varying number of twitches and at several fatigue levels, and the ultrastructural appearance was compared with that of resting fibers treated by identical fixation methods. No gross structural abnormalities were observed but subtle changes occurred. The mitochondria of stimulated fibers contain granules of normal size and number. The inner crista width is constant but the matrix width is increased on stimulation. These changes would not compromise ATP production. The myofibrils are normal except for a slight swelling in the myosin lattice. The transverse system (T system) and sarcoplasmic reticulum are intact. The minor diameter of the transverse tubule (T tubule) is increased slightly in stimulated fibers. The gap between the T-TC membranes stays constant at about 110 A, but tiny connecting pillars are seen to cross this gap more frequently in stimulated fibers (21 +/- 5% triads) than in resting fibers (8 +/- 6%). In stimulated fibers there is a marked increase in the electron dense content of the terminal cisternae (TC). Inasmuch as the observed structural changes correlate with the number of twitches but not with the fatigue level, it is concluded that TC density and T-TC pillar formation are related to the normal mechanisms of excitation-contraction coupling."}
{"id": "PMID:479819", "title": "Temporal patterns and selectivity in the unitary responses of olfactory receptors in the tiger salamander to odor stimulation.", "content": "Temporal patterns and selectivity in unitary responses of 100 single olfactory receptors in the tiger salamander to odor stimulation were investigated. An olfactometer which permitted control of stimulus concentration, duration, and flow rate was calibrated with a gas chromatograph. Stimulus pulses were monitored by recording the electroolfactogram from the surface of the olfactory epithelium. Both diphasic and triphasic spikes were recorded extracellularly. No discernible differences in types of responses, reproducibility of responses, and cross-unit distribution of spontaneous rates distinguished diphasic from triphasic units. The cross-unit selectivity in responses to the seven olfactory stimulants used and the range of odorant concentrations which effectively evoked these responses suggest variations in types and number of types of receptive sites on each cell. Temporal patterns in the unitary responses were generally less complex than those observed in the olfactory bulb. Phasic stimulations evoked phasic patterns. Tonic stimulations evoked phasic/tonic patterns. Occasionally poststimulus depressions or elevations in firing rates were observed. The nature of these patterns varied somewhat with odorant concentration for a particular unit.", "contents": "Temporal patterns and selectivity in the unitary responses of olfactory receptors in the tiger salamander to odor stimulation. Temporal patterns and selectivity in unitary responses of 100 single olfactory receptors in the tiger salamander to odor stimulation were investigated. An olfactometer which permitted control of stimulus concentration, duration, and flow rate was calibrated with a gas chromatograph. Stimulus pulses were monitored by recording the electroolfactogram from the surface of the olfactory epithelium. Both diphasic and triphasic spikes were recorded extracellularly. No discernible differences in types of responses, reproducibility of responses, and cross-unit distribution of spontaneous rates distinguished diphasic from triphasic units. The cross-unit selectivity in responses to the seven olfactory stimulants used and the range of odorant concentrations which effectively evoked these responses suggest variations in types and number of types of receptive sites on each cell. Temporal patterns in the unitary responses were generally less complex than those observed in the olfactory bulb. Phasic stimulations evoked phasic patterns. Tonic stimulations evoked phasic/tonic patterns. Occasionally poststimulus depressions or elevations in firing rates were observed. The nature of these patterns varied somewhat with odorant concentration for a particular unit."}
{"id": "PMID:479820", "title": "Adaptation and cross-adaptation to odor stimulation of olfactory receptors in the tiger salamander.", "content": "We have used the effects of self- and cross-adaptation on the unitary responses of olfactory receptors of the tiger salamander to odor stimulation to investigate the stimulus-specific components of these responses and to provide information about the cross-cell variations in the numbers and numbers of types of constitutent receptive sites. An olfactometer delivered sequential odorous pulses, either juxtaposed or separated by a variable time delay. We used four pairs of odorants judged to be similar within a given pair. The unitary response to the test stimulation relative to that of the conditioning stimulation varied from being unchanged to being completely eliminated. We sometimes observed substantial poststimulus increases in the firing rate following stimulation with juxtaposed odorous pulse. Except in the case of one odorant pair, cross-adaptation occurred both with juxtaposed pulses and with pulses separated in time. With the methyl butyrate/ethyl butyrate odorant pair, however, statistically significant cross-adaptation appeared only with juxtaposed pulses. We propose a simple model to aid in explaining these phenomena. The experimental observations in conjunction with this model are used to obtain estimates of the maximal and minimal number of receptive site types available for interaction with the chosen odorants.", "contents": "Adaptation and cross-adaptation to odor stimulation of olfactory receptors in the tiger salamander. We have used the effects of self- and cross-adaptation on the unitary responses of olfactory receptors of the tiger salamander to odor stimulation to investigate the stimulus-specific components of these responses and to provide information about the cross-cell variations in the numbers and numbers of types of constitutent receptive sites. An olfactometer delivered sequential odorous pulses, either juxtaposed or separated by a variable time delay. We used four pairs of odorants judged to be similar within a given pair. The unitary response to the test stimulation relative to that of the conditioning stimulation varied from being unchanged to being completely eliminated. We sometimes observed substantial poststimulus increases in the firing rate following stimulation with juxtaposed odorous pulse. Except in the case of one odorant pair, cross-adaptation occurred both with juxtaposed pulses and with pulses separated in time. With the methyl butyrate/ethyl butyrate odorant pair, however, statistically significant cross-adaptation appeared only with juxtaposed pulses. We propose a simple model to aid in explaining these phenomena. The experimental observations in conjunction with this model are used to obtain estimates of the maximal and minimal number of receptive site types available for interaction with the chosen odorants."}
{"id": "PMID:479821", "title": "Sodium and calcium interactions in vascular smooth muscle cells of the rabbit ear artery.", "content": "The effects of Na-free and of K-free solutions on the membrane potential, on tension development, and on 45Ca exchange have been investigated in rabbit ear artery. The contraction induced by Na-free solutions and the tension which develops in K-free solutions after a delay of about 1 h are both submaximal. Exposure for 4 h to K-free solutions does not affect the membrane potential, whereas Na-free solutions depolarize the cells by 10-20 mV, depending on the Na-substitute. Neither the amplitude nor the rate constant of the slowly exchanging 45Ca-fraction is affected by these experimental procedures. Substituting external Na by choline or TMA induces a transient increase of the 45Ca-efflux rate which does not occur in a Ca-free efflux medium, and which can be blocked with La. K readmission to Na-enriched tissues hyperpolarizes the cells up to -100 mV and induces a relaxation, without exerting any effect on the 45Ca efflux rate. The release of Ca from intracellular stores, induced by histamine and FCCP, and its subsequent extrusion through the plasma membrane produce a transient stimulation of the 45Ca efflux, which is not affected by the reduction of the Na gradient. The transient contraction induced by histamine in Ca-free solutions is affected in a different way by different Na substitutes. The results do not fit the Na-Ca exchange hypothesis but are consistent with an effect of the Na gradient on the passive Ca influx.", "contents": "Sodium and calcium interactions in vascular smooth muscle cells of the rabbit ear artery. The effects of Na-free and of K-free solutions on the membrane potential, on tension development, and on 45Ca exchange have been investigated in rabbit ear artery. The contraction induced by Na-free solutions and the tension which develops in K-free solutions after a delay of about 1 h are both submaximal. Exposure for 4 h to K-free solutions does not affect the membrane potential, whereas Na-free solutions depolarize the cells by 10-20 mV, depending on the Na-substitute. Neither the amplitude nor the rate constant of the slowly exchanging 45Ca-fraction is affected by these experimental procedures. Substituting external Na by choline or TMA induces a transient increase of the 45Ca-efflux rate which does not occur in a Ca-free efflux medium, and which can be blocked with La. K readmission to Na-enriched tissues hyperpolarizes the cells up to -100 mV and induces a relaxation, without exerting any effect on the 45Ca efflux rate. The release of Ca from intracellular stores, induced by histamine and FCCP, and its subsequent extrusion through the plasma membrane produce a transient stimulation of the 45Ca efflux, which is not affected by the reduction of the Na gradient. The transient contraction induced by histamine in Ca-free solutions is affected in a different way by different Na substitutes. The results do not fit the Na-Ca exchange hypothesis but are consistent with an effect of the Na gradient on the passive Ca influx."}
{"id": "PMID:479822", "title": "The effect of strychnine, bicuculline, and picrotoxin on X and Y cells in the cat retina.", "content": "The effect of intravenous strychnine and the GABA antagonists picrotoxin and bicuculline upon the discharge pattern of center-surround-organized cat retinal ganglion cells of X and Y type were studied. Stimuli (mostly scotopic, and some photopic) were selected such that responses from both on and off-center cells were either due to the center, due to the surround, or clearly mixed. Pre-drug control responses were obtained, and their behavior following administration of the antagonists was observed for periods up to several hours. X-cell responses were affected in a consistent manner by strychnine while being unaffected by GABA antagonists. All observed changes following strychnine were consistent with a shift in center-surround balance of X cells in favor of the center. For Y-cell responses to flashing annuli following strychnine, there was either no shift or a relatively small shift in center-surround balance. Compared to X-cell responses to flashing lights, those of Y cells were very little affected by strychnine and in most cases were unaffected. It thus appears that glycine plays a similar role in receptive field organization of X cells as does GABA in Y cells (Kirby and Enroth-Cugell, 1976. J. Gen. Physiol. 68:465-484).", "contents": "The effect of strychnine, bicuculline, and picrotoxin on X and Y cells in the cat retina. The effect of intravenous strychnine and the GABA antagonists picrotoxin and bicuculline upon the discharge pattern of center-surround-organized cat retinal ganglion cells of X and Y type were studied. Stimuli (mostly scotopic, and some photopic) were selected such that responses from both on and off-center cells were either due to the center, due to the surround, or clearly mixed. Pre-drug control responses were obtained, and their behavior following administration of the antagonists was observed for periods up to several hours. X-cell responses were affected in a consistent manner by strychnine while being unaffected by GABA antagonists. All observed changes following strychnine were consistent with a shift in center-surround balance of X cells in favor of the center. For Y-cell responses to flashing annuli following strychnine, there was either no shift or a relatively small shift in center-surround balance. Compared to X-cell responses to flashing lights, those of Y cells were very little affected by strychnine and in most cases were unaffected. It thus appears that glycine plays a similar role in receptive field organization of X cells as does GABA in Y cells (Kirby and Enroth-Cugell, 1976. J. Gen. Physiol. 68:465-484)."}
{"id": "PMID:479823", "title": "The active tension-length curve of vascular smooth muscle related to its cellular components.", "content": "The active and passive isometric tension-length (internal circumference) relation of vascular smooth muscle has been investigated using a 100-200-micron lumen diameter artery from the rat mesenteric bed. Conditions were established under which maximal activation was obtained at all lengths. Below L0 (the length at which maximum tension, delta T0, was developed) the active tension fell with decreasing length along a line which extrapolated to 0.38 L0; below 1.1 L0 the relation was reversible regardless of the protocol used. Above L0 the active tension fell linearly with increasing length along a line which extrapolated to zero tension at 1.82 L0. At the longer lengths investigated (up to 1.6 L0) the passive tension upon which the active responses were superimposed was as high as 4.4 delta T0. However, measurements of the dynamic characteristics of the preparation (with a time resolution of 2 ms) suggest that the active tension measured is nevertheless a measure of the active properties of the contractile apparatus. Direct light microscopic observation of the effect of length change on the cells within the walls of the preparation showed that changes in vessel length produced, on average, the same percentage change in cell length. Histological examination showed no signs of cell destruction following large extensions. The results suggest that the decrease in tension with extension above L0 is due to changes in the properties of the contractile apparatus, rather than to cellular damage.", "contents": "The active tension-length curve of vascular smooth muscle related to its cellular components. The active and passive isometric tension-length (internal circumference) relation of vascular smooth muscle has been investigated using a 100-200-micron lumen diameter artery from the rat mesenteric bed. Conditions were established under which maximal activation was obtained at all lengths. Below L0 (the length at which maximum tension, delta T0, was developed) the active tension fell with decreasing length along a line which extrapolated to 0.38 L0; below 1.1 L0 the relation was reversible regardless of the protocol used. Above L0 the active tension fell linearly with increasing length along a line which extrapolated to zero tension at 1.82 L0. At the longer lengths investigated (up to 1.6 L0) the passive tension upon which the active responses were superimposed was as high as 4.4 delta T0. However, measurements of the dynamic characteristics of the preparation (with a time resolution of 2 ms) suggest that the active tension measured is nevertheless a measure of the active properties of the contractile apparatus. Direct light microscopic observation of the effect of length change on the cells within the walls of the preparation showed that changes in vessel length produced, on average, the same percentage change in cell length. Histological examination showed no signs of cell destruction following large extensions. The results suggest that the decrease in tension with extension above L0 is due to changes in the properties of the contractile apparatus, rather than to cellular damage."}
{"id": "PMID:479824", "title": "Anion transport in dog, cat, and human red cells. Effects of varying cell volume and Donnan ratio.", "content": "Membrane potential and the rate constants for anion self-exchange in dog, cat, and human red blood cells have been shown to vary with cell volume. For dog and cat red cells, the outward rate constants for SO4 and Cl increase while the inward rate constant for SO4 decreases as cells swell or shrink. These changes coincide with the membrane potential becoming more negative as a result of changes in cell volume. Human red cells exhibit a similar change in the rate constants for SO4 and Cl efflux in response to cell swelling, but shrunken cells exhibit a decreased rate constant for SO4 efflux and a more positive membrane potential. Hyperpolarization of shrunken dog and cat red cells is due to a volume-dependent rate constant for SO4 efflux and a more positive membrane potential. Hyperpolarization of shrunken dog and cat red cells is due to a volume-dependent increase in PNa. If this increase in PNa is prevented by ATP depletion or if the outward Na gradient is removed, the response to shrinking is identical to human red cells. These results suggest that the volume dependence of anion permeability may be secondary to changes in the anion equilibrium ratio which in red cells is reflected by the membrane potential. When the membrane potential and cell volume of human red cells were varied independently by a method involving pretreatment with nystatin, it was found that the rate of anion transport (for SO4 and Cl) does not vary with cell volume but rather with membrane potential (anion equilibrium ratio); that is, the rate constant for anion efflux is decreased and that for influx is increased as the membrane potential becomes more positive (internal anion concentration increases) while the opposite is true with membrane hyperpolarization (a fall in internal anion concentration).", "contents": "Anion transport in dog, cat, and human red cells. Effects of varying cell volume and Donnan ratio. Membrane potential and the rate constants for anion self-exchange in dog, cat, and human red blood cells have been shown to vary with cell volume. For dog and cat red cells, the outward rate constants for SO4 and Cl increase while the inward rate constant for SO4 decreases as cells swell or shrink. These changes coincide with the membrane potential becoming more negative as a result of changes in cell volume. Human red cells exhibit a similar change in the rate constants for SO4 and Cl efflux in response to cell swelling, but shrunken cells exhibit a decreased rate constant for SO4 efflux and a more positive membrane potential. Hyperpolarization of shrunken dog and cat red cells is due to a volume-dependent rate constant for SO4 efflux and a more positive membrane potential. Hyperpolarization of shrunken dog and cat red cells is due to a volume-dependent increase in PNa. If this increase in PNa is prevented by ATP depletion or if the outward Na gradient is removed, the response to shrinking is identical to human red cells. These results suggest that the volume dependence of anion permeability may be secondary to changes in the anion equilibrium ratio which in red cells is reflected by the membrane potential. When the membrane potential and cell volume of human red cells were varied independently by a method involving pretreatment with nystatin, it was found that the rate of anion transport (for SO4 and Cl) does not vary with cell volume but rather with membrane potential (anion equilibrium ratio); that is, the rate constant for anion efflux is decreased and that for influx is increased as the membrane potential becomes more positive (internal anion concentration increases) while the opposite is true with membrane hyperpolarization (a fall in internal anion concentration)."}
{"id": "PMID:479825", "title": "Ouabain binding and coupled sodium, potassium, and chloride transport in isolated transverse tubules of skeletal muscle.", "content": "The affinity and number of binding sites of [3H]ouabain to isolated transverse (T) tubules were determined in the absence and presence of deoxycholate. In both conditions the KD was approximately 53 nM while deoxycholate increased the number of binding sites from 3.5 to 37 pmol/mg protein. We concluded that the ouabain binding sites were located primarily on the inside of the isolated vesicle and that the vesicles were impermeable to ouabain. ATP induced a highly active Na+ accumulation by the T tubules which increased Na+ in the T tubular lumen by almost 200 nmol/mg protein. The accumulation had an initial fast phase lasting 2-3 min and a subsequent slow phase which continued for at least 40 min. The rate of the initial fast phase indicated a turnover number of 20 Na+/s. The Na+ accumulation was prevented by monensin but was unaffected by valinomycin. Ouabain did not influence Na+ uptake, but digitoxin inhibited it. At low K+ the accumulation of Na+ was reduced 3.7-fold below the value at 50 mM K+. 86Rb, employed as a tracer to detect K+, showed a first phase of K+ release while Na+ was accumulated. After 2-3 min, K+ was reaccumulated while Na+ continued to increase in the lumen. T tubules accumulated Cl- on addition of ATP. This suggested that ATP initiated an exchange of Na+ for K+ followed by uptake of Na+ and K+ accompanied by Cl-.", "contents": "Ouabain binding and coupled sodium, potassium, and chloride transport in isolated transverse tubules of skeletal muscle. The affinity and number of binding sites of [3H]ouabain to isolated transverse (T) tubules were determined in the absence and presence of deoxycholate. In both conditions the KD was approximately 53 nM while deoxycholate increased the number of binding sites from 3.5 to 37 pmol/mg protein. We concluded that the ouabain binding sites were located primarily on the inside of the isolated vesicle and that the vesicles were impermeable to ouabain. ATP induced a highly active Na+ accumulation by the T tubules which increased Na+ in the T tubular lumen by almost 200 nmol/mg protein. The accumulation had an initial fast phase lasting 2-3 min and a subsequent slow phase which continued for at least 40 min. The rate of the initial fast phase indicated a turnover number of 20 Na+/s. The Na+ accumulation was prevented by monensin but was unaffected by valinomycin. Ouabain did not influence Na+ uptake, but digitoxin inhibited it. At low K+ the accumulation of Na+ was reduced 3.7-fold below the value at 50 mM K+. 86Rb, employed as a tracer to detect K+, showed a first phase of K+ release while Na+ was accumulated. After 2-3 min, K+ was reaccumulated while Na+ continued to increase in the lumen. T tubules accumulated Cl- on addition of ATP. This suggested that ATP initiated an exchange of Na+ for K+ followed by uptake of Na+ and K+ accompanied by Cl-."}
{"id": "PMID:479826", "title": "Asymmetry in the mechanism for anion exchange in human red blood cell membranes. Evidence for reciprocating sites that react with one transported anion at a time.", "content": "The kinetics of chloride and bromide transport were examined in intact human red blood cells and resealed ghosts. Because the influx and efflux of halide ions are almost equal (less than 0.01% difference), the stimulation of the exchange flux by external halides could be determined by measuring 36Cl or 82Br efflux. When the external halide concentration was increased by replacement of isoionic, isotonic solutions of sucrose and the nontransported anion citrate, the stimulation of the exchange flux was hyperbolic and was maximum at 20 mM halide externally. The K 1/2-out, the external concentration of chloride or bromide which stimulated the efflux to half of its maximum value, was 3 and 1 mM respectively, 15-fold smaller than K 1/2-in which we found to be about equal to the K 1/2 of halide self-exchange with nearly equal internal and external concentrations. Thus, the transport mechanism behaves asymmetrically with respect to these transported halides. Bromide flux was two-fold greater in bromide-chloride heteroexchange than in bromide-bromide self-exchange but it was still much smaller than the chloride self-exchange flux. The maximum influx and efflux of bromide in exchange for chloride were roughly eqal. Thus, since the maximum transport rates in the two directions are nearly equal, the kinetics of bromide equilibrium exchange with equal concentrations on the two sides are controlled on the inside where K 1/2 is greatest. The K 1/2-out Cl was a hyperbolic function of internal chloride concentration and was proportional to the maximum flux at each internal chloride concentration. These results are evaluated in terms of two broad categories of models. We conclude that, in contrast to other ion transport systems which have been shown to have kinetics of a sequential mechanism, anion exchange is compatible with a ping-pong mechanism in which a single site reciprocates between inside- and outside-facing orientations with asymmetric K 1/2 values.", "contents": "Asymmetry in the mechanism for anion exchange in human red blood cell membranes. Evidence for reciprocating sites that react with one transported anion at a time. The kinetics of chloride and bromide transport were examined in intact human red blood cells and resealed ghosts. Because the influx and efflux of halide ions are almost equal (less than 0.01% difference), the stimulation of the exchange flux by external halides could be determined by measuring 36Cl or 82Br efflux. When the external halide concentration was increased by replacement of isoionic, isotonic solutions of sucrose and the nontransported anion citrate, the stimulation of the exchange flux was hyperbolic and was maximum at 20 mM halide externally. The K 1/2-out, the external concentration of chloride or bromide which stimulated the efflux to half of its maximum value, was 3 and 1 mM respectively, 15-fold smaller than K 1/2-in which we found to be about equal to the K 1/2 of halide self-exchange with nearly equal internal and external concentrations. Thus, the transport mechanism behaves asymmetrically with respect to these transported halides. Bromide flux was two-fold greater in bromide-chloride heteroexchange than in bromide-bromide self-exchange but it was still much smaller than the chloride self-exchange flux. The maximum influx and efflux of bromide in exchange for chloride were roughly eqal. Thus, since the maximum transport rates in the two directions are nearly equal, the kinetics of bromide equilibrium exchange with equal concentrations on the two sides are controlled on the inside where K 1/2 is greatest. The K 1/2-out Cl was a hyperbolic function of internal chloride concentration and was proportional to the maximum flux at each internal chloride concentration. These results are evaluated in terms of two broad categories of models. We conclude that, in contrast to other ion transport systems which have been shown to have kinetics of a sequential mechanism, anion exchange is compatible with a ping-pong mechanism in which a single site reciprocates between inside- and outside-facing orientations with asymmetric K 1/2 values."}
{"id": "PMID:479827", "title": "Binding of scorpion toxin to receptor sites associated with sodium channels in frog muscle. Correlation of voltage-dependent binding with activation.", "content": "Purified scorpion toxin (Leiurus quinquestriatus) slows inactivation of sodium channels in frog muscle at concentrations in the range of 17-170 nM. Mono[125I]iodo scorpion toxin binds to a single class of sites in frog sartorius muscle with a dissociation constant of 14 nM and a binding capacity of 13 fmol/mg wet weight. Specific binding is inhibited more than 90% by 3 microM sea anemone toxin II and by depolarization with 165 mM K+. Half-maximal inhibition of binding is observed on depolarization to -41 mV. The voltage dependence of scorpion toxin binding is correlated with the voltage dependence of activation of sodium channels. Removal of calcium from the bathing medium shifts both activation and inhibition of scorpion toxin binding to more negative membrane potentials. The results are considered in terms of the hypothesis that activation of sodium channels causes a conformational change in the scorpion toxin receptor site resulting in reduced affinity for scorpion toxin.", "contents": "Binding of scorpion toxin to receptor sites associated with sodium channels in frog muscle. Correlation of voltage-dependent binding with activation. Purified scorpion toxin (Leiurus quinquestriatus) slows inactivation of sodium channels in frog muscle at concentrations in the range of 17-170 nM. Mono[125I]iodo scorpion toxin binds to a single class of sites in frog sartorius muscle with a dissociation constant of 14 nM and a binding capacity of 13 fmol/mg wet weight. Specific binding is inhibited more than 90% by 3 microM sea anemone toxin II and by depolarization with 165 mM K+. Half-maximal inhibition of binding is observed on depolarization to -41 mV. The voltage dependence of scorpion toxin binding is correlated with the voltage dependence of activation of sodium channels. Removal of calcium from the bathing medium shifts both activation and inhibition of scorpion toxin binding to more negative membrane potentials. The results are considered in terms of the hypothesis that activation of sodium channels causes a conformational change in the scorpion toxin receptor site resulting in reduced affinity for scorpion toxin."}
{"id": "PMID:479828", "title": "Calcium measurement in the periphery of an axon.", "content": "Aequorin was microinjected into squid giant axons, the axons were stimulated, and the change in light emission was followed. This response was compared with that found when the axon, in addition to being microinjected with aequorin, is also injected with the dye phenol red. Large concentrations of phenol red injected into axons result in a high probability that photons emitted by aequorin, when it reacts with Ca in the core of the axoplasm, will be absorbed before they escape from the axon; photons produced by the aequorin reaction at the periphery of the axoplasm are much less likely to be absorbed. This technique thus favors observing changes in Cai taking place in the periphery of the axon. Stimulation in 50 mM Ca seawater of an aequorin-phenol red-injected axon at 180 s-1 for 1 min produces a scarcely detectable change in Cai; the addition of 2 mM cyanide (CN) to the seawater produces an easily measureable increase in Cai, suggesting that mitochondrial buffering in the periphery is substantial. Making the pH of the axoplasm of a normal axon alkaline with 30 mM NH4+ -50 mM Ca seawater, reduces the resting glow of the axon but results in an even more rapid increase in Cai with stimulation. In a phenol red-injected axon, this treatment results in a measureable response to stimulation in the absence of CN.", "contents": "Calcium measurement in the periphery of an axon. Aequorin was microinjected into squid giant axons, the axons were stimulated, and the change in light emission was followed. This response was compared with that found when the axon, in addition to being microinjected with aequorin, is also injected with the dye phenol red. Large concentrations of phenol red injected into axons result in a high probability that photons emitted by aequorin, when it reacts with Ca in the core of the axoplasm, will be absorbed before they escape from the axon; photons produced by the aequorin reaction at the periphery of the axoplasm are much less likely to be absorbed. This technique thus favors observing changes in Cai taking place in the periphery of the axon. Stimulation in 50 mM Ca seawater of an aequorin-phenol red-injected axon at 180 s-1 for 1 min produces a scarcely detectable change in Cai; the addition of 2 mM cyanide (CN) to the seawater produces an easily measureable increase in Cai, suggesting that mitochondrial buffering in the periphery is substantial. Making the pH of the axoplasm of a normal axon alkaline with 30 mM NH4+ -50 mM Ca seawater, reduces the resting glow of the axon but results in an even more rapid increase in Cai with stimulation. In a phenol red-injected axon, this treatment results in a measureable response to stimulation in the absence of CN."}
{"id": "PMID:479829", "title": "Comparison of proteins from Mycobacterium fortuitum, Mycobacterium nonchromogenicum and Mycobacterium terrae using flat bed electrophoresis.", "content": "Polyacrylamide gel electrophoresis of bacterial lysates in a flat bed gives a linear relationship between 1n mol. wt of the proteins and the square root of their migration distances, thereby allowing standardization of different electrophoresis runs and precise comparison between homologous bands. The results obtained with Mycobacterium fortuitum, M. terrae and M. nonchromogenicum strains were used in numerical analysis. Mycobacterium fortuitum and M. nonchromogenicum showed a greater internal similarity than M. terrae, while two strains of the latter clustered with M. nonchromogenicum. The method described allows the comparison of mycobacteria with different generation times and provides a large number of good characters for numerical taxonomy.", "contents": "Comparison of proteins from Mycobacterium fortuitum, Mycobacterium nonchromogenicum and Mycobacterium terrae using flat bed electrophoresis. Polyacrylamide gel electrophoresis of bacterial lysates in a flat bed gives a linear relationship between 1n mol. wt of the proteins and the square root of their migration distances, thereby allowing standardization of different electrophoresis runs and precise comparison between homologous bands. The results obtained with Mycobacterium fortuitum, M. terrae and M. nonchromogenicum strains were used in numerical analysis. Mycobacterium fortuitum and M. nonchromogenicum showed a greater internal similarity than M. terrae, while two strains of the latter clustered with M. nonchromogenicum. The method described allows the comparison of mycobacteria with different generation times and provides a large number of good characters for numerical taxonomy."}
{"id": "PMID:479830", "title": "Antigenic cross-reactivity of major outer membrane proteins in enterobacteriaceae species.", "content": "The protein constituents in the outer membrane (OM) of several serotypes of Escherichia coli and some other Enterobacteriaceae cross-reacted antigenically. Solubilized OM preparations of these bacteria were applied in interfacial precipitin tests to antisera elicited in rabbits against whole bacterial cells, absorbed with their appropriate lipopolysaccharide before testing. The resulting immunecomplexes were analysed on polyacrylamide gels. Protein profiles of the immunoprecipitates showed a considerable antigenic cross-reactivity of outer membrane proteins between most E. coli serotypes. Cross-reactivity, though substantially lower, was also found with OM from three other Enterobacteriaceae species, but was not detectable with Pseudomonas aeruginosa OM. When OM preparations were solubilized at room temperature, the peptidoglycan-bound proteins in the molecular weight range 37,000 to 41,000 predominated in the protein profiles of the immunecomplexes. In profiles of immunecomplexes obtained with boiled OM preparations, a heat-modifiable protein (mol. wt 33,000) predominated. The major OM proteins of the Gram-negative bacterium may therefore play a role as common surface antigens of the family of Enterobacteriaceae.", "contents": "Antigenic cross-reactivity of major outer membrane proteins in enterobacteriaceae species. The protein constituents in the outer membrane (OM) of several serotypes of Escherichia coli and some other Enterobacteriaceae cross-reacted antigenically. Solubilized OM preparations of these bacteria were applied in interfacial precipitin tests to antisera elicited in rabbits against whole bacterial cells, absorbed with their appropriate lipopolysaccharide before testing. The resulting immunecomplexes were analysed on polyacrylamide gels. Protein profiles of the immunoprecipitates showed a considerable antigenic cross-reactivity of outer membrane proteins between most E. coli serotypes. Cross-reactivity, though substantially lower, was also found with OM from three other Enterobacteriaceae species, but was not detectable with Pseudomonas aeruginosa OM. When OM preparations were solubilized at room temperature, the peptidoglycan-bound proteins in the molecular weight range 37,000 to 41,000 predominated in the protein profiles of the immunecomplexes. In profiles of immunecomplexes obtained with boiled OM preparations, a heat-modifiable protein (mol. wt 33,000) predominated. The major OM proteins of the Gram-negative bacterium may therefore play a role as common surface antigens of the family of Enterobacteriaceae."}
{"id": "PMID:479831", "title": "Autoradiographic study of the localization and evolution of growth zones in bacterial colonies.", "content": "Incorporation of [3H]leucine in the bacteria of 18 to 48 h-old colonies of Pseudomonas aeruginosa, Pseudomonas putida, Bacillus thuringiensis, Staphylococcus aureus and Escherichia coli enabled the localization of bacterial multiplication sites by means of autoradiography of sagittal sections. In colonies where fast diameter expansion occurred, all the bacteria from the peripheral corona contributed to peripheral growth; in colonies where the expansion was slower, the growth rate of the bacteria in this region was heterogeneous. Besides this peripheral growth, a central region of bacterial multiplication was always found, but with variable localization and extension. In aerobic species, such as P. aeruginosa and P. putida, the central growth site was limited to the zone of oxygen penetration into the bacterial mass. However, in facultatively anaerobic species, bacterial multiplication dependend on nutrient supply. For 48 h-old colonies of S. aureus, a more complex localization of growth seemed to be affected simultaneously by nutrient penetration and accumulation of toxic substances.", "contents": "Autoradiographic study of the localization and evolution of growth zones in bacterial colonies. Incorporation of [3H]leucine in the bacteria of 18 to 48 h-old colonies of Pseudomonas aeruginosa, Pseudomonas putida, Bacillus thuringiensis, Staphylococcus aureus and Escherichia coli enabled the localization of bacterial multiplication sites by means of autoradiography of sagittal sections. In colonies where fast diameter expansion occurred, all the bacteria from the peripheral corona contributed to peripheral growth; in colonies where the expansion was slower, the growth rate of the bacteria in this region was heterogeneous. Besides this peripheral growth, a central region of bacterial multiplication was always found, but with variable localization and extension. In aerobic species, such as P. aeruginosa and P. putida, the central growth site was limited to the zone of oxygen penetration into the bacterial mass. However, in facultatively anaerobic species, bacterial multiplication dependend on nutrient supply. For 48 h-old colonies of S. aureus, a more complex localization of growth seemed to be affected simultaneously by nutrient penetration and accumulation of toxic substances."}
{"id": "PMID:479833", "title": "Characteristics of a lipolytic and fatty acid-requiring Butyrivibrio sp. isolated from the ovine rumen.", "content": "A naturally occurring fatty acid-requiring Butyrivibrio sp. (strain S2), isolated from the ovine rumen, deacylates plant galactolipids, phospholipids and sulpholipids to obtain sufficient fatty acid for growth. Growth in vitro was promoted by adding to the growth medium a single straight-chain saturated fatty acid (C13 to C18) or vaccenic acid. Palmitoleic and oleic acids also supported growth but gave lengthy lag phases probably due to their toxicity. Linolenic and linoleic acids supported good growth but they were completely hydrogenated to trans-11-octadecenoic acid which was incorporated into the bacterial complex lipids. No chain elongation, chain shortening or desaturation of the added fatty acids occurred and all were substantially incorporated into bacterial lipids of the plasmalogen type, partially as a new type of hydrophobic grouping derived from two molecules of fatty acid. The absence of fatty acid unsaturation poses the question of the maintenance of membrane fluidity within this bacterium.", "contents": "Characteristics of a lipolytic and fatty acid-requiring Butyrivibrio sp. isolated from the ovine rumen. A naturally occurring fatty acid-requiring Butyrivibrio sp. (strain S2), isolated from the ovine rumen, deacylates plant galactolipids, phospholipids and sulpholipids to obtain sufficient fatty acid for growth. Growth in vitro was promoted by adding to the growth medium a single straight-chain saturated fatty acid (C13 to C18) or vaccenic acid. Palmitoleic and oleic acids also supported growth but gave lengthy lag phases probably due to their toxicity. Linolenic and linoleic acids supported good growth but they were completely hydrogenated to trans-11-octadecenoic acid which was incorporated into the bacterial complex lipids. No chain elongation, chain shortening or desaturation of the added fatty acids occurred and all were substantially incorporated into bacterial lipids of the plasmalogen type, partially as a new type of hydrophobic grouping derived from two molecules of fatty acid. The absence of fatty acid unsaturation poses the question of the maintenance of membrane fluidity within this bacterium."}
{"id": "PMID:479834", "title": "Glucan-binding proteins of Streptococcus mutans serotype c.", "content": "Three glucan-binding proteins have been isolated from the extracellular fluid cultures of Streptococcus mutans serotype c. These proteins were adsorbed to glucans containing 1,3-alpha or 1,6-alpha bonds and linked to various chromatographic supports: they were eluted from columns by a dextran solution. Glucosyltransferase activity was associated with two of the glucan-binding proteins.", "contents": "Glucan-binding proteins of Streptococcus mutans serotype c. Three glucan-binding proteins have been isolated from the extracellular fluid cultures of Streptococcus mutans serotype c. These proteins were adsorbed to glucans containing 1,3-alpha or 1,6-alpha bonds and linked to various chromatographic supports: they were eluted from columns by a dextran solution. Glucosyltransferase activity was associated with two of the glucan-binding proteins."}
{"id": "PMID:479835", "title": "Numerical taxonomy of Actinomadura and related actinomycetes.", "content": "One hundred and fifty-six Actinomadura strains, marker strains of related taxa, and related isolates from bagasse and fodder were the subject of numerical phenetic analyses using 90 unit characters. The data were examined using the simple matching (SSM), Jaccard (SJ) and pattern (DP) coefficients and clustering was achieved using both single and average linkage algorithms. Cluster composition was not markedly affected either by the coefficient or clustering algorithms used or by test error, estimated at 4.5%. Actinomadura dassonvillei, Actinomadura madurae and Streptomyces somaliensis formed good taxospecies, but the separation of Actinomadura pelletieri strains into two clusters by SJ and SSM analysis requires further study. The single representatives of Actinomadura helvata, Actinomadura pusilla, Actinomadura roseoviolacea, Actinomadura spadix and Actinomadura verrucosopora seemed to form new centres of variation while Actinomadura citrea and Actinomadura malachitica showed much similarity with Actinomadura madurae. Most of the isolates form bagasse and fodder were recovered in two well-defined phena, provisionally labelled clusters 'A' and 'B' which showed little similarity to either Actinomadura or Nocardia strains. The effect of the different coefficients on the aggregation of clusters is discussed.", "contents": "Numerical taxonomy of Actinomadura and related actinomycetes. One hundred and fifty-six Actinomadura strains, marker strains of related taxa, and related isolates from bagasse and fodder were the subject of numerical phenetic analyses using 90 unit characters. The data were examined using the simple matching (SSM), Jaccard (SJ) and pattern (DP) coefficients and clustering was achieved using both single and average linkage algorithms. Cluster composition was not markedly affected either by the coefficient or clustering algorithms used or by test error, estimated at 4.5%. Actinomadura dassonvillei, Actinomadura madurae and Streptomyces somaliensis formed good taxospecies, but the separation of Actinomadura pelletieri strains into two clusters by SJ and SSM analysis requires further study. The single representatives of Actinomadura helvata, Actinomadura pusilla, Actinomadura roseoviolacea, Actinomadura spadix and Actinomadura verrucosopora seemed to form new centres of variation while Actinomadura citrea and Actinomadura malachitica showed much similarity with Actinomadura madurae. Most of the isolates form bagasse and fodder were recovered in two well-defined phena, provisionally labelled clusters 'A' and 'B' which showed little similarity to either Actinomadura or Nocardia strains. The effect of the different coefficients on the aggregation of clusters is discussed."}
{"id": "PMID:479836", "title": "Trimethylamine oxide: a terminal electron acceptor in anaerobic respiration of bacteria.", "content": "Trimethylamine oxide (TMAO) stimulated both the anaerobic growth rate and the growth yield of Proteus NTHC 153. The molar growth yield from glucose and pyruvate in tryptone/yeast extract medium doubled in the presence of TMAO, and the organism grew anaerobically on the non-fermentable substrates L-lactate and formate when TMAO was added to the medium. We conclude that TMAO stimulated growth by serving as a terminal electron acceptor in an oxidative phosphorylation process.", "contents": "Trimethylamine oxide: a terminal electron acceptor in anaerobic respiration of bacteria. Trimethylamine oxide (TMAO) stimulated both the anaerobic growth rate and the growth yield of Proteus NTHC 153. The molar growth yield from glucose and pyruvate in tryptone/yeast extract medium doubled in the presence of TMAO, and the organism grew anaerobically on the non-fermentable substrates L-lactate and formate when TMAO was added to the medium. We conclude that TMAO stimulated growth by serving as a terminal electron acceptor in an oxidative phosphorylation process."}
{"id": "PMID:479837", "title": "The control of the synthesis of isocitrate lyase in a thermophilic bacillus.", "content": "From a strain of Bacillus stearothermophilus, devoid of active pyruvate carboxylase, a mutant (NG-15) was selected that grew on acetate in the presence of glucose. This mutant differed from its parent organism in possessing high activities of isocitrate lyase when grown on all carbon sources tested except nutrient broth, in possessing unusually low activities of NADP+-dependent isocitrate dehydrogenase and in containing increased amounts of isocitrate. Revertants of mutant NG-15 which regained the ability to synthesize active pyruvate carboxylase also synthesized isocitrate lyase and isocitrate dehydrogenase to the same extent as the wild-type strain. These results suggest that the regulatory mechanism for the synthesis of isocitrate lyase in the thermophile may be different from that in mesophilic bacilli.", "contents": "The control of the synthesis of isocitrate lyase in a thermophilic bacillus. From a strain of Bacillus stearothermophilus, devoid of active pyruvate carboxylase, a mutant (NG-15) was selected that grew on acetate in the presence of glucose. This mutant differed from its parent organism in possessing high activities of isocitrate lyase when grown on all carbon sources tested except nutrient broth, in possessing unusually low activities of NADP+-dependent isocitrate dehydrogenase and in containing increased amounts of isocitrate. Revertants of mutant NG-15 which regained the ability to synthesize active pyruvate carboxylase also synthesized isocitrate lyase and isocitrate dehydrogenase to the same extent as the wild-type strain. These results suggest that the regulatory mechanism for the synthesis of isocitrate lyase in the thermophile may be different from that in mesophilic bacilli."}
{"id": "PMID:479838", "title": "Influence of oxygen tension on nitrate reduction by a Klebsiella sp. growing in chemostat culture.", "content": "At dissolved oxygen tensions of 15 mmHg (2 kPa) and below, nitrate-limited continuous cultures of Klebsiella K312 synthesized nitrate reductase (NR) and nitrite reductase (NiR) and excreted ammonia. Under anaerobic conditions over 60% of the nitrate-nitrogen utilized was excreted as ammonia. In contrast, carbon-limited cultures excreted nitrite at dissolved oxygen tensions of 15 mmHg or below and synthesized NR but not NiR. Ammonia repressed neither NR nor NiR synthesis. These observations indicate that below a critical oxygen tension of 15 mmHg Klebsiella K312 utilizes oxygen and nitrate as electron acceptors. This oxygen tension correlates well with the critical oxygen tension observed for a change from oxidative to fermentative metabolism in cultures of Klebsiella aerogenes. The product of dissimilatory nitrate reduction is ammonia in nitrate-limited cultures but principally nitrite in carbon-limited (nitrate excess) cultures.", "contents": "Influence of oxygen tension on nitrate reduction by a Klebsiella sp. growing in chemostat culture. At dissolved oxygen tensions of 15 mmHg (2 kPa) and below, nitrate-limited continuous cultures of Klebsiella K312 synthesized nitrate reductase (NR) and nitrite reductase (NiR) and excreted ammonia. Under anaerobic conditions over 60% of the nitrate-nitrogen utilized was excreted as ammonia. In contrast, carbon-limited cultures excreted nitrite at dissolved oxygen tensions of 15 mmHg or below and synthesized NR but not NiR. Ammonia repressed neither NR nor NiR synthesis. These observations indicate that below a critical oxygen tension of 15 mmHg Klebsiella K312 utilizes oxygen and nitrate as electron acceptors. This oxygen tension correlates well with the critical oxygen tension observed for a change from oxidative to fermentative metabolism in cultures of Klebsiella aerogenes. The product of dissimilatory nitrate reduction is ammonia in nitrate-limited cultures but principally nitrite in carbon-limited (nitrate excess) cultures."}
{"id": "PMID:479839", "title": "The components of Mycoplasma salivarium and its growth medium that are responsible for film formation.", "content": "Studies on film production by mycoplasmas revealed that film was produced by completely disintegrated mycoplasma cells on Noble agar in the presence of horse serum. Film production was due to an enzymic reaction between mycoplasma lipase, possibly phospholipase A, and phospholipid in serum.", "contents": "The components of Mycoplasma salivarium and its growth medium that are responsible for film formation. Studies on film production by mycoplasmas revealed that film was produced by completely disintegrated mycoplasma cells on Noble agar in the presence of horse serum. Film production was due to an enzymic reaction between mycoplasma lipase, possibly phospholipase A, and phospholipid in serum."}
{"id": "PMID:479840", "title": "Description of strains of Peptostreptococcus anaerobius isolated from subcutaneous abscesses in cats.", "content": "Strains of Peptostreptococcus, Streptococcus and of a Gram-positive coccus, which was initially isolated as an anaerobe but grew subsequently as a facultative organism, were isolated from subcutaneous abscesses in cats. The cat strains of Peptostreptococcus gave metabolic fermentation products in combinations described for P. anaerobius. The Streptococcus strains conformed to the group S. intermedius. The facultative organism described had the metabolic products of P. anaerobius but the distinctly different biochemical characteristics of S. intermedius and fits neither of the genera strictly.", "contents": "Description of strains of Peptostreptococcus anaerobius isolated from subcutaneous abscesses in cats. Strains of Peptostreptococcus, Streptococcus and of a Gram-positive coccus, which was initially isolated as an anaerobe but grew subsequently as a facultative organism, were isolated from subcutaneous abscesses in cats. The cat strains of Peptostreptococcus gave metabolic fermentation products in combinations described for P. anaerobius. The Streptococcus strains conformed to the group S. intermedius. The facultative organism described had the metabolic products of P. anaerobius but the distinctly different biochemical characteristics of S. intermedius and fits neither of the genera strictly."}
{"id": "PMID:479841", "title": "Investigation of recombinants of human influenza and fowl plague viruses.", "content": "Recombinants of human influenza type A viruses, A/Krasnodar/101/1959 (H2N2) or A/Habarovsk/15/1976 (H3N2), and fowl plague virus (FPV), strain Weybridge (Hav1Neq1) were obtained. The genome of the recombinant obtained by recombination of influenza A/Habarovsk/15/1976 virus and FPV contained the genes 4 (HA) and 6 (NA) derived from the influenza A/Habarovsk virus and all the other genes [1, 2, 3, 5 (NP), 7 (M), 8 (NS)] from FPV. The genome of the recombinant of A/Krasnodar/101/1959 virus and FPV contained the genes 2, 4 (HA) and 6 (NA) derived from influenza A/Krasnodar virus and all the other genes [1, 3, 5, (NP), 7 (M), 8 (NS)] from FPV. The recombinants, like FPV, gave high virus yields in chick embryos and could multiply at high temperatures (40 and 42 degrees C), but, like human influenza viruses, were non-pathogenic for chickens and did not replicate in chick embryo fibroblast culture, but did replicate in a human conjunctiva cell line, clone 1-5C-4. The virion transcriptase of the recombinants, in a number of properties determined in vitro, was similar to FPV transcriptase but not to the human influenza virus enzyme.", "contents": "Investigation of recombinants of human influenza and fowl plague viruses. Recombinants of human influenza type A viruses, A/Krasnodar/101/1959 (H2N2) or A/Habarovsk/15/1976 (H3N2), and fowl plague virus (FPV), strain Weybridge (Hav1Neq1) were obtained. The genome of the recombinant obtained by recombination of influenza A/Habarovsk/15/1976 virus and FPV contained the genes 4 (HA) and 6 (NA) derived from the influenza A/Habarovsk virus and all the other genes [1, 2, 3, 5 (NP), 7 (M), 8 (NS)] from FPV. The genome of the recombinant of A/Krasnodar/101/1959 virus and FPV contained the genes 2, 4 (HA) and 6 (NA) derived from influenza A/Krasnodar virus and all the other genes [1, 3, 5, (NP), 7 (M), 8 (NS)] from FPV. The recombinants, like FPV, gave high virus yields in chick embryos and could multiply at high temperatures (40 and 42 degrees C), but, like human influenza viruses, were non-pathogenic for chickens and did not replicate in chick embryo fibroblast culture, but did replicate in a human conjunctiva cell line, clone 1-5C-4. The virion transcriptase of the recombinants, in a number of properties determined in vitro, was similar to FPV transcriptase but not to the human influenza virus enzyme."}
{"id": "PMID:479842", "title": "High molecular weight virus DNA in KB cells infected with ts mutants of adenovirus type 2 under permissive and non-permissive conditions.", "content": "Temperature-sensitive (ts) mutants of adenovirus type 2 (Ad2), which are deficient in virus DNA synthesis at the non-permissive temperature, have been used to investigate whether virus DNA replication is required for the occurrence of high mol. wt. Ad2 DNA (greater than 100S, 50 to 90S) in human cells productively infected with Ad2. The high mol. wt. virus DNA has been previously shown to consist of virus and cellular DNA molecules covalently linked. The present data indicate that after infection with DNA-ts mutants, the production of high mol.wt. virus DNA is much less sensitive to restrictive conditions than the synthesis of unit length (34S) Ad2 DNA. This finding lends further support to the idea that the occurrence of high mol. wt. virus DNA is independent of the synthesis of unit length virus DNA.", "contents": "High molecular weight virus DNA in KB cells infected with ts mutants of adenovirus type 2 under permissive and non-permissive conditions. Temperature-sensitive (ts) mutants of adenovirus type 2 (Ad2), which are deficient in virus DNA synthesis at the non-permissive temperature, have been used to investigate whether virus DNA replication is required for the occurrence of high mol. wt. Ad2 DNA (greater than 100S, 50 to 90S) in human cells productively infected with Ad2. The high mol. wt. virus DNA has been previously shown to consist of virus and cellular DNA molecules covalently linked. The present data indicate that after infection with DNA-ts mutants, the production of high mol.wt. virus DNA is much less sensitive to restrictive conditions than the synthesis of unit length (34S) Ad2 DNA. This finding lends further support to the idea that the occurrence of high mol. wt. virus DNA is independent of the synthesis of unit length virus DNA."}
{"id": "PMID:479843", "title": "Studies on the mechanism of influenza virus entry into cells.", "content": "Inhibitors of glycolysis, oxidative phosphorylation, protein synthesis, membrane Na&-K& transport and microfilament and microtubule function have been employed to elucidate the mechanism of influenza virus uptake by CAM and CEF cells. Electron microscopy demonstrated uptake of virus by viropexis in the presence of all these inhibitors. Utilizing a pulse labelling technique, virus entering CEF cells in the presence of inhibitors was shown to initiate specific virus polypeptide synthesis after neutralization of remaining extracellular virus and removal of the inhibitors. As a consequence of these findings an energy independent mechanism of viropexis has been proposed.", "contents": "Studies on the mechanism of influenza virus entry into cells. Inhibitors of glycolysis, oxidative phosphorylation, protein synthesis, membrane Na&-K& transport and microfilament and microtubule function have been employed to elucidate the mechanism of influenza virus uptake by CAM and CEF cells. Electron microscopy demonstrated uptake of virus by viropexis in the presence of all these inhibitors. Utilizing a pulse labelling technique, virus entering CEF cells in the presence of inhibitors was shown to initiate specific virus polypeptide synthesis after neutralization of remaining extracellular virus and removal of the inhibitors. As a consequence of these findings an energy independent mechanism of viropexis has been proposed."}
{"id": "PMID:479844", "title": "Proteins specified by togaviruses in infected Aedes albopictus (Singh) mosquito cells.", "content": "Yields of greater than 10(7) p.f.u./ml at 28 or 37 degrees C of the alphavirus Sindbis and the flavivirus Kunjin were obtained in the Aedes Albopictus (Singh) cell line, the latent periods being 4 to 6 and 10 to 12 h, respectively. Despite a high background of host protein synthesis, virtually all the virus-specified proteins of the flaviviruses Kunjin, Dengue-2 and Japanese encephalitis were labelled and resolved by slab gel electrophoresis of infected and uninfected cell proteins. In contrast, only one induced protein, of mol. wt. 30 000, was identified in cells labelled during Sindbis virus infection. The envelope glycoprotein V3 of Kunjin virus was resolved as a double band in samples of infected cytoplasm labelled with 3H-glucosamine, similar to that of carbohydrate-labelled V3 in vertebrate (Vero) cells. Attempts to reduce host protein synthesis selectively during labelling periods were unsuccessful using either a hypertonic inhibition block or treatment with 0.1 mu g actinomycin D per ml. The most efficient labelling of Kunjin virus-specified proteins was achieved at 37 degrees C in the presence of actinomycin D. The largest non-structural flavivirus protein NV5 migrated slightly faster than NV5 from infected vertebrate (Vero) cells. The small non-structural proteins NV1, NV1 1/2 and NV2 from infected mosquito cells were successively trimmed during post translational periods exceeding 70 min, compared to much shorter periods reported previously for post translational modifications of these proteins in vertebrate cells.", "contents": "Proteins specified by togaviruses in infected Aedes albopictus (Singh) mosquito cells. Yields of greater than 10(7) p.f.u./ml at 28 or 37 degrees C of the alphavirus Sindbis and the flavivirus Kunjin were obtained in the Aedes Albopictus (Singh) cell line, the latent periods being 4 to 6 and 10 to 12 h, respectively. Despite a high background of host protein synthesis, virtually all the virus-specified proteins of the flaviviruses Kunjin, Dengue-2 and Japanese encephalitis were labelled and resolved by slab gel electrophoresis of infected and uninfected cell proteins. In contrast, only one induced protein, of mol. wt. 30 000, was identified in cells labelled during Sindbis virus infection. The envelope glycoprotein V3 of Kunjin virus was resolved as a double band in samples of infected cytoplasm labelled with 3H-glucosamine, similar to that of carbohydrate-labelled V3 in vertebrate (Vero) cells. Attempts to reduce host protein synthesis selectively during labelling periods were unsuccessful using either a hypertonic inhibition block or treatment with 0.1 mu g actinomycin D per ml. The most efficient labelling of Kunjin virus-specified proteins was achieved at 37 degrees C in the presence of actinomycin D. The largest non-structural flavivirus protein NV5 migrated slightly faster than NV5 from infected vertebrate (Vero) cells. The small non-structural proteins NV1, NV1 1/2 and NV2 from infected mosquito cells were successively trimmed during post translational periods exceeding 70 min, compared to much shorter periods reported previously for post translational modifications of these proteins in vertebrate cells."}
{"id": "PMID:479845", "title": "Study of a new strain of paramyxoviruses isolated from wild ducks: structural polypeptides.", "content": "By polyacrylamide gel electrophoresis, Duck/Mississippi/75 virus was shown to contain five different types of polypeptides of mol. wt. ranging from 76 X 1O(3) to 43 X 10(3), two of which were glycosylated (mol. wt. 76 X 10(3) and 57 X 10(3). A comparison of this data with similar results obtained using Yucaipa and Newcastle disease virus (NDV) revealed a similarity with NDV, concerning the number, position and mol. wt. of the polypeptides. Haemagglutinating and neuraminidase properties are associated with surface glycoproteins which represent at least 40% of the virus protein. After KCl and Triton X-100 treatment and centrifugation on linear sucrose density gradients (10 to 25%, w/w) it was possible to isolate glycoprotein VP1 of mol. wt 76 X 10(3) with which haemagglutinating and neuraminidase activities were associated.", "contents": "Study of a new strain of paramyxoviruses isolated from wild ducks: structural polypeptides. By polyacrylamide gel electrophoresis, Duck/Mississippi/75 virus was shown to contain five different types of polypeptides of mol. wt. ranging from 76 X 1O(3) to 43 X 10(3), two of which were glycosylated (mol. wt. 76 X 10(3) and 57 X 10(3). A comparison of this data with similar results obtained using Yucaipa and Newcastle disease virus (NDV) revealed a similarity with NDV, concerning the number, position and mol. wt. of the polypeptides. Haemagglutinating and neuraminidase properties are associated with surface glycoproteins which represent at least 40% of the virus protein. After KCl and Triton X-100 treatment and centrifugation on linear sucrose density gradients (10 to 25%, w/w) it was possible to isolate glycoprotein VP1 of mol. wt 76 X 10(3) with which haemagglutinating and neuraminidase activities were associated."}
{"id": "PMID:479846", "title": "The effect of inhibitors of glycosylation on interferon production in human lymphoblastoid cells.", "content": "Interferon production was inhibited in the Namalwa line of human lymphoblastoid cells by treatment with 2-deoxy-D-glucose or D-glucosamine. D-Glucosamine also inhibited protein synthesis and the cells were no longer viable, whereas 2-deoxy-D-glucose allowed protein and RNA synthesis to continue at control rates, and the cells remained fully viable. It is concluded that a glycosylation step is essential for production of lymphoblastoid interferon.", "contents": "The effect of inhibitors of glycosylation on interferon production in human lymphoblastoid cells. Interferon production was inhibited in the Namalwa line of human lymphoblastoid cells by treatment with 2-deoxy-D-glucose or D-glucosamine. D-Glucosamine also inhibited protein synthesis and the cells were no longer viable, whereas 2-deoxy-D-glucose allowed protein and RNA synthesis to continue at control rates, and the cells remained fully viable. It is concluded that a glycosylation step is essential for production of lymphoblastoid interferon."}
{"id": "PMID:479847", "title": "Molecular species of interferon induced in mouse L cells by Newcastle disease virus and polyriboinosinic-polyribocytidylic acid.", "content": "Interferons were stimulated in mouse L cells by Newcastle disease virus (NDV) or by polyriboinosinic-polyribocytidylic acid poly(rI).poly(rC). These were fractionated by sequential affinity chromatography on bovine plasma albumin (BPA)-Sepharose and on omega-carboxypentyl (CH)-Sepharose. Based on their interaction with CH-Sepharose, interferon induced by NDV was resolved into three major bands of activity (L/NDV-1,2,3) and poly(rI).poly(rC)-interferon into two (L/rI:rC-1,2). These interferon components were purified to a specific activity of 3 X 10(7) to 4 X 10(7) units/mg protein by antibody affinity chromatography and examined by electrophoresis in SDS-polyacrylamide gels. A total of five molecular species was thus identified for NDV-induced interferon and three for poly(rI).poly(rC) induced interferon, as summarized in Table 1. We conclude from our observations that mouse interferons can be produced by L cells in multiple forms with specific physiochemical properties and in proportions determined by the type of agent employed for induction.", "contents": "Molecular species of interferon induced in mouse L cells by Newcastle disease virus and polyriboinosinic-polyribocytidylic acid. Interferons were stimulated in mouse L cells by Newcastle disease virus (NDV) or by polyriboinosinic-polyribocytidylic acid poly(rI).poly(rC). These were fractionated by sequential affinity chromatography on bovine plasma albumin (BPA)-Sepharose and on omega-carboxypentyl (CH)-Sepharose. Based on their interaction with CH-Sepharose, interferon induced by NDV was resolved into three major bands of activity (L/NDV-1,2,3) and poly(rI).poly(rC)-interferon into two (L/rI:rC-1,2). These interferon components were purified to a specific activity of 3 X 10(7) to 4 X 10(7) units/mg protein by antibody affinity chromatography and examined by electrophoresis in SDS-polyacrylamide gels. A total of five molecular species was thus identified for NDV-induced interferon and three for poly(rI).poly(rC) induced interferon, as summarized in Table 1. We conclude from our observations that mouse interferons can be produced by L cells in multiple forms with specific physiochemical properties and in proportions determined by the type of agent employed for induction."}
{"id": "PMID:479848", "title": "Characterization of two temperature-sensitive mutants of adenovirus type 5.", "content": "The properties of two temperature-sensitive mutants ts 18 and ts 19 of adenovirus type 5 were studied. It was demonstrated that they had a defect such that they failed to assemble virus and showed defective processing of infected cell polypeptides at the restrictive temperature. Analysis, after protease digestion, of the virions produced at the permissive temperature by SDS PAGE, and of the substrate availability of the mutants to the virus protein kinase suggested that polypeptide VI was defective in these mutants.", "contents": "Characterization of two temperature-sensitive mutants of adenovirus type 5. The properties of two temperature-sensitive mutants ts 18 and ts 19 of adenovirus type 5 were studied. It was demonstrated that they had a defect such that they failed to assemble virus and showed defective processing of infected cell polypeptides at the restrictive temperature. Analysis, after protease digestion, of the virions produced at the permissive temperature by SDS PAGE, and of the substrate availability of the mutants to the virus protein kinase suggested that polypeptide VI was defective in these mutants."}
{"id": "PMID:479849", "title": "The toxic effect of double-stranded RNA for interferon-treated cells: evidence for a heterogeneous cellular response and the role of the cell nucleus.", "content": "When L929 cells were treated with interferon and subsequently with poly(rI).poly(rC), there was a pronounced toxic effect. Most of the cells lysed, but some survived and grew at the same rate as control cells to yield cells which were as sensitive to the effects of interferon and poly(rI).poly(rC) as the original population. The proportion of surviving cells did not vary with either the cell cycle or the cell density. The treated cells produced interferon and some of the interferon was produced by the resistant cells. Cells which had been X-irradiated before treatment with interferon and poly(rI).poly(rC) behaved similarly so cell division was not necessary for the development of toxicity. The toxic effect also developed when cells were enucleated with the aid of cytochalas in B after treatment with interferon, but not if they were enucleated before treatment. It is concluded that the nucleus is essential for interferon to exert its effect on the cells, but not for the development of cytotoxicity after the addition of poly(rI).poly(rC).", "contents": "The toxic effect of double-stranded RNA for interferon-treated cells: evidence for a heterogeneous cellular response and the role of the cell nucleus. When L929 cells were treated with interferon and subsequently with poly(rI).poly(rC), there was a pronounced toxic effect. Most of the cells lysed, but some survived and grew at the same rate as control cells to yield cells which were as sensitive to the effects of interferon and poly(rI).poly(rC) as the original population. The proportion of surviving cells did not vary with either the cell cycle or the cell density. The treated cells produced interferon and some of the interferon was produced by the resistant cells. Cells which had been X-irradiated before treatment with interferon and poly(rI).poly(rC) behaved similarly so cell division was not necessary for the development of toxicity. The toxic effect also developed when cells were enucleated with the aid of cytochalas in B after treatment with interferon, but not if they were enucleated before treatment. It is concluded that the nucleus is essential for interferon to exert its effect on the cells, but not for the development of cytotoxicity after the addition of poly(rI).poly(rC)."}
{"id": "PMID:479850", "title": "The toxic effect of double-stranded RNA for interferon-treated L cells: studies on membrane transport and with cell-free systems.", "content": "We have tested two possible explanations for the toxic effects observed in L cells treated with interferon followed by poly(rI).poly(rC), namely (1) that the toxicity was preceded by and due to a change in membrane permeability which could be monitored by measurement of ion fluxes, or (2) that the toxicity was due to the action of the inhibitors of protein synthesis which are known to be formed when extracts from interferon-treated cells are treated with poly(rI).poly(rC) in vitro. Neither explanation was found to be correct.", "contents": "The toxic effect of double-stranded RNA for interferon-treated L cells: studies on membrane transport and with cell-free systems. We have tested two possible explanations for the toxic effects observed in L cells treated with interferon followed by poly(rI).poly(rC), namely (1) that the toxicity was preceded by and due to a change in membrane permeability which could be monitored by measurement of ion fluxes, or (2) that the toxicity was due to the action of the inhibitors of protein synthesis which are known to be formed when extracts from interferon-treated cells are treated with poly(rI).poly(rC) in vitro. Neither explanation was found to be correct."}
{"id": "PMID:479851", "title": "Copper chelate affinity chromatography of human fibroblast and leucocyte interferons.", "content": "Human fibroblast and human leucocyte interferons display a strong affinity for the copper chelate of bis-carboxymethyl amino agarose, binding tenaciously over a wide pH range (7.4 to 4.0). Their binding is apparently irreversible on a sorbent saturated with copper (24.8 mumol of Cu2+/ml of column bed). However, both interferons can be partially recovered from sorbents of lower copper content, prepared by leaching the columns with sodium citrate at pH 9.0. The recovery of fibroblast interferon from a leached sorbent (5.8 mumol of Cu2+/ml of column bed) is about 30% and that of leucocyte interferon about 60%. Moreover, the strength of binding of leucocyte interferon can be modulated by leaching copper chelate-agarose with citrate of varying concentration.", "contents": "Copper chelate affinity chromatography of human fibroblast and leucocyte interferons. Human fibroblast and human leucocyte interferons display a strong affinity for the copper chelate of bis-carboxymethyl amino agarose, binding tenaciously over a wide pH range (7.4 to 4.0). Their binding is apparently irreversible on a sorbent saturated with copper (24.8 mumol of Cu2+/ml of column bed). However, both interferons can be partially recovered from sorbents of lower copper content, prepared by leaching the columns with sodium citrate at pH 9.0. The recovery of fibroblast interferon from a leached sorbent (5.8 mumol of Cu2+/ml of column bed) is about 30% and that of leucocyte interferon about 60%. Moreover, the strength of binding of leucocyte interferon can be modulated by leaching copper chelate-agarose with citrate of varying concentration."}
{"id": "PMID:479852", "title": "Stimulation of recombination in phage T4 by nitrous acid-induced lesions.", "content": "Recombination of phage T4 can be stimulated more than sixfold above the spontaneous level by treatment with nitrous acid, indicating that the lesions induced by this agent are strongly recombinogenic. Two temperature sensitive mutants defective in exonuclease functions showed less than the wild-type spontaneous level of recombination even after nitrous acid treatment and a ligase mutant showed a highly elevated frequency of recombination after treatment. Since these same mutants have analogous effects on spontaneous recombination, the results imply that nitrous acid-induced lesions in DNA stimulate a recombinational repair process similar in some of its enzymic steps to spontaneous recombination.", "contents": "Stimulation of recombination in phage T4 by nitrous acid-induced lesions. Recombination of phage T4 can be stimulated more than sixfold above the spontaneous level by treatment with nitrous acid, indicating that the lesions induced by this agent are strongly recombinogenic. Two temperature sensitive mutants defective in exonuclease functions showed less than the wild-type spontaneous level of recombination even after nitrous acid treatment and a ligase mutant showed a highly elevated frequency of recombination after treatment. Since these same mutants have analogous effects on spontaneous recombination, the results imply that nitrous acid-induced lesions in DNA stimulate a recombinational repair process similar in some of its enzymic steps to spontaneous recombination."}
{"id": "PMID:479853", "title": "[Do male germ cells begin meiosis during fetal life?].", "content": "The existence of a preleptotene chromosome condensation and decondensation stage occuring between the last premeiotic interphase and the leptotene stage was described in numberous plants. This stage was also reported in the human fetal oocyte and in various animals (rabbit, sheep, mouse). A similar process of chromosome condensation was described in the human foetal testis, but in this latter, the decondensation phase leading to leptotene was never observed. According to this observation and to various experimental results from the literature, the preleptotene condensation stage could be related to the processes of meiotic initiation. It could represent a phase of transition between mitotic and meiotic behaviour during which the germinal cell could be sensitive to meiosis-inducing factors. It is suggested that the male germinal cell 46,XY could enter meiosis. This hypothesis is confirmed by the observation of the capacity of the XY germ cell in the mouse to become an oocyte. This capacity is normally not expressed, due to the repressive control of the adjacent Sertoli cells. Thus, stimulation of the germinal cell to enter meiosis could result from environmental factors rather than from a genetic programmation.", "contents": "[Do male germ cells begin meiosis during fetal life?]. The existence of a preleptotene chromosome condensation and decondensation stage occuring between the last premeiotic interphase and the leptotene stage was described in numberous plants. This stage was also reported in the human fetal oocyte and in various animals (rabbit, sheep, mouse). A similar process of chromosome condensation was described in the human foetal testis, but in this latter, the decondensation phase leading to leptotene was never observed. According to this observation and to various experimental results from the literature, the preleptotene condensation stage could be related to the processes of meiotic initiation. It could represent a phase of transition between mitotic and meiotic behaviour during which the germinal cell could be sensitive to meiosis-inducing factors. It is suggested that the male germinal cell 46,XY could enter meiosis. This hypothesis is confirmed by the observation of the capacity of the XY germ cell in the mouse to become an oocyte. This capacity is normally not expressed, due to the repressive control of the adjacent Sertoli cells. Thus, stimulation of the germinal cell to enter meiosis could result from environmental factors rather than from a genetic programmation."}
{"id": "PMID:479854", "title": "[Structural abnormalities of the Y chromosome. Observations in ten cases].", "content": "The authors report ten cases of structural anomalies involving the Y chromosome: five cases of a dicentric Y chromosome, one ring Y chromosome, one case of a Y isochromosome containing the long arms, one deletion of the long arms (Yq-), one case of an abnormally long Y chromosome (Yq+) and one Y-autosome translocation. Analysis of clinical and chromosomal correlations, especially with respect to sexual differentiation, led to discussing the role of the Y chromosome.", "contents": "[Structural abnormalities of the Y chromosome. Observations in ten cases]. The authors report ten cases of structural anomalies involving the Y chromosome: five cases of a dicentric Y chromosome, one ring Y chromosome, one case of a Y isochromosome containing the long arms, one deletion of the long arms (Yq-), one case of an abnormally long Y chromosome (Yq+) and one Y-autosome translocation. Analysis of clinical and chromosomal correlations, especially with respect to sexual differentiation, led to discussing the role of the Y chromosome."}
{"id": "PMID:479855", "title": "[Congenital cataract linked to the Y chromosome].", "content": "A possible Y linked form of congenital cataract is reported, but an autosomal dominant mode of inheritance cannot be ruled out.", "contents": "[Congenital cataract linked to the Y chromosome]. A possible Y linked form of congenital cataract is reported, but an autosomal dominant mode of inheritance cannot be ruled out."}
{"id": "PMID:479857", "title": "Attenuated influenza produced by experimental intranasal inoculation.", "content": "To assess the relative effect of natural versus experimental influenza illness on pulmonary function, we compared 43 normal adults with documented nonpneumonic influenza A infection during three outbreaks, 1974 (A/Port Chalmers/74), 1975 (A/Port Chalmers/74), and 1976 (A/Victoria/75) to 24 normal volunteers following nasal inoculation with wild-type influenza A/England/42/72, A/Scotland/74 or A/Victoria/75. In naturally acquired illness, abnormalities in small airway functiion and transiently increase airway reactivity were observed. In contrast, no such dysfunction was observed in experimentally induced illness. This group manifested milder illness and significantly shorter duration of cough.", "contents": "Attenuated influenza produced by experimental intranasal inoculation. To assess the relative effect of natural versus experimental influenza illness on pulmonary function, we compared 43 normal adults with documented nonpneumonic influenza A infection during three outbreaks, 1974 (A/Port Chalmers/74), 1975 (A/Port Chalmers/74), and 1976 (A/Victoria/75) to 24 normal volunteers following nasal inoculation with wild-type influenza A/England/42/72, A/Scotland/74 or A/Victoria/75. In naturally acquired illness, abnormalities in small airway functiion and transiently increase airway reactivity were observed. In contrast, no such dysfunction was observed in experimentally induced illness. This group manifested milder illness and significantly shorter duration of cough."}
{"id": "PMID:479858", "title": "The absence of differences in cellular immunity in elderly individuals with and without delayed local cutaneous reactions to influenza vaccine.", "content": "Two groups of elderly subjects who received bivalent influenza virus vaccine were identified. Those in Group I manifested marked local reactions, suggestive of delayed hypersensitivity reactions, whereas those in Group II, matched with Group I for vaccine preparation, had no cutaneous reactions. In vitro assays of immune function were performed on pairs of subjects from the two groups and included serum immunoglobulin and complement levels, antibody response to vaccine, lymphocyte transformation to mitogens (phytohemagglutinin, pokeweed mitogen and concanavalin A) and antigens (streptokinase-streptodornase, influenza virus vaccine antigens and multiple mixed lymphocytes), and polymorphonuclear leukocyte chemotactic responsiveness. There was no demonstrable correlation between delayed local cutaneous reaction and preservation of in vitro cell-mediated immune function in these elderly individuals.", "contents": "The absence of differences in cellular immunity in elderly individuals with and without delayed local cutaneous reactions to influenza vaccine. Two groups of elderly subjects who received bivalent influenza virus vaccine were identified. Those in Group I manifested marked local reactions, suggestive of delayed hypersensitivity reactions, whereas those in Group II, matched with Group I for vaccine preparation, had no cutaneous reactions. In vitro assays of immune function were performed on pairs of subjects from the two groups and included serum immunoglobulin and complement levels, antibody response to vaccine, lymphocyte transformation to mitogens (phytohemagglutinin, pokeweed mitogen and concanavalin A) and antigens (streptokinase-streptodornase, influenza virus vaccine antigens and multiple mixed lymphocytes), and polymorphonuclear leukocyte chemotactic responsiveness. There was no demonstrable correlation between delayed local cutaneous reaction and preservation of in vitro cell-mediated immune function in these elderly individuals."}
{"id": "PMID:479859", "title": "Clinical evaluation of a rubella passive hemagglutination test system.", "content": "A recently marketed passive hemagglutination (PHA) test (Rubacell, Abbott Laboratories) was compared to the hemagglutination inhibition (HI) test for the detection of antibody to rubella virus. The performance of the PHA system in determining immunity to rubella was evaluated by comparing the PHA results and HI results on 1, 086 randomly selected sera submitted for routine premarital and prenatal testing. Of the 1, 079 specimens assayable by both procedures, 1, 053 of the results (97.6%) were in agreement on initial testing. When the 26 initially discrepant specimens were retested for clarification, there was final agreement in 1, 067 of the specimens (98.9%). Twelve specimens were classified as persistently discrepant (five were not retested by PHA) and seven were unassayable by HI. Seven of the specimens with discrepant results were PHA positive and HI negative, and five were PHA negative and HI positive. Discussion of the two tests with respect to technical difficulty, cost, and controls is also included.", "contents": "Clinical evaluation of a rubella passive hemagglutination test system. A recently marketed passive hemagglutination (PHA) test (Rubacell, Abbott Laboratories) was compared to the hemagglutination inhibition (HI) test for the detection of antibody to rubella virus. The performance of the PHA system in determining immunity to rubella was evaluated by comparing the PHA results and HI results on 1, 086 randomly selected sera submitted for routine premarital and prenatal testing. Of the 1, 079 specimens assayable by both procedures, 1, 053 of the results (97.6%) were in agreement on initial testing. When the 26 initially discrepant specimens were retested for clarification, there was final agreement in 1, 067 of the specimens (98.9%). Twelve specimens were classified as persistently discrepant (five were not retested by PHA) and seven were unassayable by HI. Seven of the specimens with discrepant results were PHA positive and HI negative, and five were PHA negative and HI positive. Discussion of the two tests with respect to technical difficulty, cost, and controls is also included."}
{"id": "PMID:479860", "title": "HBeAg and anti-HBe detection by radioimmunoassay: correlation with vertical transmission of hepatitis B virus in Taiwan.", "content": "A sensitive radioimmunoassay technique was used to detect hepatitis B e antigen (HBeAg). A strong correlation was found between HBeAg positivity of the serum of hepatitis B surface antigen (HBsAg) carrier women in Taiwan and the subsequent development of surface antigenemia in their babies. All babies who became chronic HBsAg carriers were born to HBeAg positive women, maternal HBeAg positivity being a better prior indication of chronic antigenemia developing in the baby than the HBsAg titer in the mother's serum.", "contents": "HBeAg and anti-HBe detection by radioimmunoassay: correlation with vertical transmission of hepatitis B virus in Taiwan. A sensitive radioimmunoassay technique was used to detect hepatitis B e antigen (HBeAg). A strong correlation was found between HBeAg positivity of the serum of hepatitis B surface antigen (HBsAg) carrier women in Taiwan and the subsequent development of surface antigenemia in their babies. All babies who became chronic HBsAg carriers were born to HBeAg positive women, maternal HBeAg positivity being a better prior indication of chronic antigenemia developing in the baby than the HBsAg titer in the mother's serum."}
{"id": "PMID:479861", "title": "Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis.", "content": "Non-A, non-B viral hepatitis was transmitted to four colony-born chimpanzees by infusion of three lots of antihemophilic factor (factor VIII) implicated in the transmission of non-A, non-B hepatitis to two human recipients. All four inoculated animals showed histopathological evidence of viral hepatitis, and all demonstrated significant ALT elevations between seven and one-half weeks after inoculation. Acute-phase plasma from one of the infected chimpanzees (no. 771) was shown to induce non-A, non-B hepatitis in two other chimpanzees approximately three weeks after their inoculation. In addition, an acute-phase open liver wedge biopsy obtained from animal no. 771 was processed and examined by immune electron microscopy (IEM) for virus-like particles with convalescent serum from a serologically confirmed case of non-A, non-B hepatitis. Twenty-five to 30 nm (mean = 27 nm) diameter virus-like particles that were either \"full\" or \"empty\" were identified in this liver preparation by IEM. Two additional chimpanzees inoculated with a cesium chloride gradient fraction of an isopycnically banded liver homogenate (animal no. 771) also developed elevated ALT activity two to two and one-half weeks later. Our findings have experimentally verified that commercially produced factor VIII materials can induce non-A, non-B hepatitis in champanzees and that the disease can be subpassaged in these animals by inoculation of either acute-phase plasma or liver. These results also provide evidence for the association of 27 nm-diameter virus-like particles with non-A, non-B viral hepatitis.", "contents": "Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis. Non-A, non-B viral hepatitis was transmitted to four colony-born chimpanzees by infusion of three lots of antihemophilic factor (factor VIII) implicated in the transmission of non-A, non-B hepatitis to two human recipients. All four inoculated animals showed histopathological evidence of viral hepatitis, and all demonstrated significant ALT elevations between seven and one-half weeks after inoculation. Acute-phase plasma from one of the infected chimpanzees (no. 771) was shown to induce non-A, non-B hepatitis in two other chimpanzees approximately three weeks after their inoculation. In addition, an acute-phase open liver wedge biopsy obtained from animal no. 771 was processed and examined by immune electron microscopy (IEM) for virus-like particles with convalescent serum from a serologically confirmed case of non-A, non-B hepatitis. Twenty-five to 30 nm (mean = 27 nm) diameter virus-like particles that were either \"full\" or \"empty\" were identified in this liver preparation by IEM. Two additional chimpanzees inoculated with a cesium chloride gradient fraction of an isopycnically banded liver homogenate (animal no. 771) also developed elevated ALT activity two to two and one-half weeks later. Our findings have experimentally verified that commercially produced factor VIII materials can induce non-A, non-B hepatitis in champanzees and that the disease can be subpassaged in these animals by inoculation of either acute-phase plasma or liver. These results also provide evidence for the association of 27 nm-diameter virus-like particles with non-A, non-B viral hepatitis."}
{"id": "PMID:479862", "title": "ECHOvirus 33 replication in human peripheral white blood cells.", "content": "ECHOvirus 33 isolated from the cerebrospinal fluid of a patient with agammaglobulinemia was shown to replicate in vitro in peripheral leukocyte suspensions from a normal antibody negative donor. Replication was demonstrated by use of sequential dilution experiments. Washed glass adherent mononuclear cell cultures from a normal antibody negative donor were also capable of supporting ECHOvirus 33 replication in vitro. Leukocyte suspensions from the infected agammaglobulinemic patient extinguished detectable infectious virus in vitro.", "contents": "ECHOvirus 33 replication in human peripheral white blood cells. ECHOvirus 33 isolated from the cerebrospinal fluid of a patient with agammaglobulinemia was shown to replicate in vitro in peripheral leukocyte suspensions from a normal antibody negative donor. Replication was demonstrated by use of sequential dilution experiments. Washed glass adherent mononuclear cell cultures from a normal antibody negative donor were also capable of supporting ECHOvirus 33 replication in vitro. Leukocyte suspensions from the infected agammaglobulinemic patient extinguished detectable infectious virus in vitro."}
{"id": "PMID:479863", "title": "Evaluation of Aedes albopictus tissue culture for use in association with arbovirus isolation.", "content": "The susceptibility and sensitivity of Aedes albopictus cell cultures to five different primary and four different low-passage arboviruses were tested. Yellow fever, West Nile, Ilesha, eastern equine encephalitis, and Flanders viruses replicated in A albopictus tissue cultures. Replication was determined by the ability of selected tissue culture fluids to infect suckling mice, and by recovery from tissue culture fluid of progressively increasing amounts of complement-fixing (CF) antigen with time. Virus persistence was demonstrated with Nodamura, western equine encephalitis, and Mayaro viruses, but multiplication was not proven; neither persistence nor multiplication was demonstrated with a Kemerovo group virus. When yellow fever and Ilesha viruses were simultaneously inoculated into A albopictus culture, CF antigen for each was consistently detected. In a more detailed comparative study of field specimens, 12 unpassaged strains of yellow fever virus were tested for infectivity in A albopictus tissue culture, Vero cells, and baby mice. Higher titers of virus were detected (0.8--2.3 log ID50 per ml) in Vero cell culture than in A albopictus tissue culture or baby mouse systems. These results suggest the feasibility of using A albopictus cells in association with the primary isolation of arboviruses.", "contents": "Evaluation of Aedes albopictus tissue culture for use in association with arbovirus isolation. The susceptibility and sensitivity of Aedes albopictus cell cultures to five different primary and four different low-passage arboviruses were tested. Yellow fever, West Nile, Ilesha, eastern equine encephalitis, and Flanders viruses replicated in A albopictus tissue cultures. Replication was determined by the ability of selected tissue culture fluids to infect suckling mice, and by recovery from tissue culture fluid of progressively increasing amounts of complement-fixing (CF) antigen with time. Virus persistence was demonstrated with Nodamura, western equine encephalitis, and Mayaro viruses, but multiplication was not proven; neither persistence nor multiplication was demonstrated with a Kemerovo group virus. When yellow fever and Ilesha viruses were simultaneously inoculated into A albopictus culture, CF antigen for each was consistently detected. In a more detailed comparative study of field specimens, 12 unpassaged strains of yellow fever virus were tested for infectivity in A albopictus tissue culture, Vero cells, and baby mice. Higher titers of virus were detected (0.8--2.3 log ID50 per ml) in Vero cell culture than in A albopictus tissue culture or baby mouse systems. These results suggest the feasibility of using A albopictus cells in association with the primary isolation of arboviruses."}
{"id": "PMID:479865", "title": "Role loss and working-class manic depression.", "content": "This first part of a multiphase longitudinal study looks at life events implicated in manic depression among an American working class, bipolar sample. Three turning points are identified. a) Stressful life events occurred that resulted in loss of significant social roles. b) Attempts to regain or replace the roles or otherwise \"fight back\" proved ineffective. c) Retreat via depression, and rebellion via mania, ensued. These turning points were well verified for 75 per cent of the subjects prior to first onset, and for 56 per cent prior to most recent onset. Special methodological issues are raised, and the need for a biopsychosocial etiology of manic depression is suggested.", "contents": "Role loss and working-class manic depression. This first part of a multiphase longitudinal study looks at life events implicated in manic depression among an American working class, bipolar sample. Three turning points are identified. a) Stressful life events occurred that resulted in loss of significant social roles. b) Attempts to regain or replace the roles or otherwise \"fight back\" proved ineffective. c) Retreat via depression, and rebellion via mania, ensued. These turning points were well verified for 75 per cent of the subjects prior to first onset, and for 56 per cent prior to most recent onset. Special methodological issues are raised, and the need for a biopsychosocial etiology of manic depression is suggested."}
{"id": "PMID:479866", "title": "The Minnesota Multiphasic Personality Inventory in predicting response to pharmacotherapy of neurotic outpatients.", "content": "The utility of the Minnesota Multiphasic Personality Inventory (MMPI) in predicting treatment response to pharmacotherapy for a group of 54 anxious and 43 depressed outpatients was examined. Discriminant function analyses using the MMPI scales were conducted on groups of improved and unimproved patients. Several significant function, as well as zero-order, differences were found. In general, improved patients scored significantly lower on scales reflecting depression and obsessive-compulsive or schizoid tendencies. They also obtained lower scores on scales measuring interpersonal sensitivity and suggestive of character traits such as low frustration tolerance, impulsivity, and resentment toward authority figures. Additional analyses in which several different profile types were compared for treatment outcome revealed few differences among groups.", "contents": "The Minnesota Multiphasic Personality Inventory in predicting response to pharmacotherapy of neurotic outpatients. The utility of the Minnesota Multiphasic Personality Inventory (MMPI) in predicting treatment response to pharmacotherapy for a group of 54 anxious and 43 depressed outpatients was examined. Discriminant function analyses using the MMPI scales were conducted on groups of improved and unimproved patients. Several significant function, as well as zero-order, differences were found. In general, improved patients scored significantly lower on scales reflecting depression and obsessive-compulsive or schizoid tendencies. They also obtained lower scores on scales measuring interpersonal sensitivity and suggestive of character traits such as low frustration tolerance, impulsivity, and resentment toward authority figures. Additional analyses in which several different profile types were compared for treatment outcome revealed few differences among groups."}
{"id": "PMID:479867", "title": "Status epilepticus. An uncommon hysterical conversion syndrome.", "content": "The distinction between hysterical and organically determined epileptic seizures may be more difficult than is sometimes supposed, and misdiagnosis can have dire consequences. Hysterical seizures may on occasions present as life-threatening crises requiring urgent and intensive treatment. Three such cases, each of which received the diagnosis of status epilepticus, are reported, and their management is discussed.", "contents": "Status epilepticus. An uncommon hysterical conversion syndrome. The distinction between hysterical and organically determined epileptic seizures may be more difficult than is sometimes supposed, and misdiagnosis can have dire consequences. Hysterical seizures may on occasions present as life-threatening crises requiring urgent and intensive treatment. Three such cases, each of which received the diagnosis of status epilepticus, are reported, and their management is discussed."}
{"id": "PMID:479868", "title": "The interrelationship between self-image disparity and social competence, defensive style, and adjustment status.", "content": "Groups of 40 psychiatric and 40 nonpsychiatric male patients were subdivided into equal groups of high and low social competence. Each patient completed a task battery which included three measures of self-image disparity and the Byrne repression-sensitization scale. High competence patients of both types were found to have higher self-image disparities than low competence patients. Psychiatric patients were found to have higher disparity scores than nonpsychiatric patients, although some evidence indicated that this was true only for the low competence groups. Higher scores on the Byrne scale (indicating sensitization) were found for high as compared to low competence patients, and for the psychiatric as compared to nonpsychiatric groups. Defensive style correlated significantly with each of the self-image measures. The results were discussed in the context of both developmental and Rogerian formulations. It was concluded that an individual's maturational level influences both self-image and defensive style, even when the individual is judged psychologically maladjusted.", "contents": "The interrelationship between self-image disparity and social competence, defensive style, and adjustment status. Groups of 40 psychiatric and 40 nonpsychiatric male patients were subdivided into equal groups of high and low social competence. Each patient completed a task battery which included three measures of self-image disparity and the Byrne repression-sensitization scale. High competence patients of both types were found to have higher self-image disparities than low competence patients. Psychiatric patients were found to have higher disparity scores than nonpsychiatric patients, although some evidence indicated that this was true only for the low competence groups. Higher scores on the Byrne scale (indicating sensitization) were found for high as compared to low competence patients, and for the psychiatric as compared to nonpsychiatric groups. Defensive style correlated significantly with each of the self-image measures. The results were discussed in the context of both developmental and Rogerian formulations. It was concluded that an individual's maturational level influences both self-image and defensive style, even when the individual is judged psychologically maladjusted."}
{"id": "PMID:479870", "title": "And if that doesn't work, what next ...? A study of treatment failures in schizophrenia.", "content": "A systematic study of schizophrenic patients who did not respond satisfactorily to one of five different forms of treatment given under controlled conditions showed that almost all of them responded satisfactorily to subsequent treatment with the combination of ataraxic drugs and group psychotherapy. Whatever the original form of treatment, and despite subsequent retreatment with drugs and group psychotherapy, there was a treatment-resistant core--a few patients who either responded very slowly or who improved relatively little.", "contents": "And if that doesn't work, what next ...? A study of treatment failures in schizophrenia. A systematic study of schizophrenic patients who did not respond satisfactorily to one of five different forms of treatment given under controlled conditions showed that almost all of them responded satisfactorily to subsequent treatment with the combination of ataraxic drugs and group psychotherapy. Whatever the original form of treatment, and despite subsequent retreatment with drugs and group psychotherapy, there was a treatment-resistant core--a few patients who either responded very slowly or who improved relatively little."}
{"id": "PMID:479871", "title": "Postpartum mania in bipolar manic-depressive patients withdrawn from lithium carbonate.", "content": "Three women, previously diagnosed as bipolar I manic-depressive, were withdrawn from lithium carbonate prophylaxis immediately prior to their pregnancies. The patients had been euthymic while on lithium carbonate for at least 3 1/2 years prior to their pregnancies. Two of the three patients developed a manic syndrome within 2 weeks postpartum. The use of lithium carbonate during pregnancy, and particularly in the postpartum period, requires reassessment. We advocate an ongoing clinical relationship and the reinstitution of lithium in the third trimester in most cases.", "contents": "Postpartum mania in bipolar manic-depressive patients withdrawn from lithium carbonate. Three women, previously diagnosed as bipolar I manic-depressive, were withdrawn from lithium carbonate prophylaxis immediately prior to their pregnancies. The patients had been euthymic while on lithium carbonate for at least 3 1/2 years prior to their pregnancies. Two of the three patients developed a manic syndrome within 2 weeks postpartum. The use of lithium carbonate during pregnancy, and particularly in the postpartum period, requires reassessment. We advocate an ongoing clinical relationship and the reinstitution of lithium in the third trimester in most cases."}
{"id": "PMID:479872", "title": "Opposite effects of naloxone on substance P-induced changes in brain DOPA synthesis and in locomotor activity in rats.", "content": "Intracerebroventricular injection of substance P (SP) enhanced the synthesis of dopamine and noradrenaline (measured as increase in DOPA formation after inhibition of the aromatic L-amino-acid decarboxylase) in rat brain. These biochemical effects were blocked in most brain regions by pretreatment with naloxone. SP also induced vasodilation, salivation and increased locomotor activity. These effects were not antagonized, but, in the case of locomotor activity, potentiated by naloxone. The data suggest the existence of specific SP-containing neuronal pathways for behavior, which pathways are not related to those regulating the synthesis of brain catecholamines.", "contents": "Opposite effects of naloxone on substance P-induced changes in brain DOPA synthesis and in locomotor activity in rats. Intracerebroventricular injection of substance P (SP) enhanced the synthesis of dopamine and noradrenaline (measured as increase in DOPA formation after inhibition of the aromatic L-amino-acid decarboxylase) in rat brain. These biochemical effects were blocked in most brain regions by pretreatment with naloxone. SP also induced vasodilation, salivation and increased locomotor activity. These effects were not antagonized, but, in the case of locomotor activity, potentiated by naloxone. The data suggest the existence of specific SP-containing neuronal pathways for behavior, which pathways are not related to those regulating the synthesis of brain catecholamines."}
{"id": "PMID:479873", "title": "Dopamine, noradrenaline and 3,4-dihydroxyphenylacetic acid (DOPAC) levels of individual brain nuclei, effects of haloperidol and pargyline.", "content": "Noradrenaline (NA), dopamine (DA) and DOPAC were determined with a newly developed radioenzymatic method simultaneously in the striatum, limbic system, hypothalamus and in catecholamine-containing cell groups of the rat brain. Only a loose relationship could be established between DOPAC and DA contents in the various brain areas. The lowest relative DOPAC level (DOPAC/DA ratio) was found in the median emience, while it was the highest in the periventricular nucleus of the hypothalamus. Haloperidol increased the DOPAC level in only part of the nuclei examined (striatum, olfactory tubercle, central amygdaloid nucleus), while in other limbic regions as well as in the hypothalamic dorsomedial, arcuate and paraventricular nuclei it proved to be ineffective. The DOPAC level in the locus coeruleus was decreased by haloperidol. Pargyline caused an appr. 50% decrease of DOPAC content of most of the nuclei in 10 min; the effectivity of the drug did not show parallelism with that of haloperidol. The monoamine oxidase inhibition caused no change in the DOPAC level in the hypothalamic periventricular and paraventricular nuclei. Results are discussed as a consequence of different reactivity of various DA-ergic terminals and catecholamine cell bodies to haloperidol and pargyline.", "contents": "Dopamine, noradrenaline and 3,4-dihydroxyphenylacetic acid (DOPAC) levels of individual brain nuclei, effects of haloperidol and pargyline. Noradrenaline (NA), dopamine (DA) and DOPAC were determined with a newly developed radioenzymatic method simultaneously in the striatum, limbic system, hypothalamus and in catecholamine-containing cell groups of the rat brain. Only a loose relationship could be established between DOPAC and DA contents in the various brain areas. The lowest relative DOPAC level (DOPAC/DA ratio) was found in the median emience, while it was the highest in the periventricular nucleus of the hypothalamus. Haloperidol increased the DOPAC level in only part of the nuclei examined (striatum, olfactory tubercle, central amygdaloid nucleus), while in other limbic regions as well as in the hypothalamic dorsomedial, arcuate and paraventricular nuclei it proved to be ineffective. The DOPAC level in the locus coeruleus was decreased by haloperidol. Pargyline caused an appr. 50% decrease of DOPAC content of most of the nuclei in 10 min; the effectivity of the drug did not show parallelism with that of haloperidol. The monoamine oxidase inhibition caused no change in the DOPAC level in the hypothalamic periventricular and paraventricular nuclei. Results are discussed as a consequence of different reactivity of various DA-ergic terminals and catecholamine cell bodies to haloperidol and pargyline."}
{"id": "PMID:479874", "title": "A highly sensitive and precise radioenzymatic assay for plasma epinephrine and norepinephrine.", "content": "A modification of the procedures for simultaneous radiometric determinations of plasma epinephrine (E) and norepinephrine (NE) was developed. High performance liquid chromatography (HPLC) is excellently suited to separate the radioactive products of the enzymatic methylation of catecholamines. The time-saving procedure shows significant advantages as against the conventional method of isolating normetanephrine and metanephrine by thin layer chromatography. The high sensitivity (detection limit = 1 pg), specifity, and good precision (cv = 6%) of this assay are demonstrated.", "contents": "A highly sensitive and precise radioenzymatic assay for plasma epinephrine and norepinephrine. A modification of the procedures for simultaneous radiometric determinations of plasma epinephrine (E) and norepinephrine (NE) was developed. High performance liquid chromatography (HPLC) is excellently suited to separate the radioactive products of the enzymatic methylation of catecholamines. The time-saving procedure shows significant advantages as against the conventional method of isolating normetanephrine and metanephrine by thin layer chromatography. The high sensitivity (detection limit = 1 pg), specifity, and good precision (cv = 6%) of this assay are demonstrated."}
{"id": "PMID:479875", "title": "An in situ study of the hypothalamo-neurohypophysial complex of the freshwater teleost Ompok bimaculatus (Bloch) with a note on the tetrapodan features of its vascularization.", "content": "In Ompok bimaculatus the neurosecretory axons of both the pars magnocellularis and pars parvocellularis component of the nucleus preopticus contribute to the formation of the left and right main tracts. At rostral levels, one third of the tracts are loosely built forming a king of curtain, while they become more compact at caudal levels. The caudal two thirds of the main tracts give off several pairs of lateral tracts which join at the midline to form the paired median tracts. The median and the main tract jointly enter the pituitary as a common tract which, on entering the proximal pars distalis, separates into two and enters the pars intermedia. The hypothalamic and the hypophysial arteries take their origin from the internal carotid artery. The former contribute to the formation of the primary capillary plexus of the median eminence, whereas the latter enter the pituitary directly and ramify in the neuroadeno-interface. The primary capillary plexus is in close topographical contact with the neurosecretory axonal complex where the median and common tracts are formed. At this site at least some axons might have their endings on the capillary plexus. Thus, this region my be comparable in structure to the median eminence of the tetrapods. The neurons of the nucleus lateralis tuberis of O. bimaculatus are AF-positive. The infundibular cavity extends into the main neurohypophysial trunk and is lined by ependymal cells like the third ventricle.", "contents": "An in situ study of the hypothalamo-neurohypophysial complex of the freshwater teleost Ompok bimaculatus (Bloch) with a note on the tetrapodan features of its vascularization. In Ompok bimaculatus the neurosecretory axons of both the pars magnocellularis and pars parvocellularis component of the nucleus preopticus contribute to the formation of the left and right main tracts. At rostral levels, one third of the tracts are loosely built forming a king of curtain, while they become more compact at caudal levels. The caudal two thirds of the main tracts give off several pairs of lateral tracts which join at the midline to form the paired median tracts. The median and the main tract jointly enter the pituitary as a common tract which, on entering the proximal pars distalis, separates into two and enters the pars intermedia. The hypothalamic and the hypophysial arteries take their origin from the internal carotid artery. The former contribute to the formation of the primary capillary plexus of the median eminence, whereas the latter enter the pituitary directly and ramify in the neuroadeno-interface. The primary capillary plexus is in close topographical contact with the neurosecretory axonal complex where the median and common tracts are formed. At this site at least some axons might have their endings on the capillary plexus. Thus, this region my be comparable in structure to the median eminence of the tetrapods. The neurons of the nucleus lateralis tuberis of O. bimaculatus are AF-positive. The infundibular cavity extends into the main neurohypophysial trunk and is lined by ependymal cells like the third ventricle."}
{"id": "PMID:479876", "title": "Influence of fluoride on the uptake of 51Cr-chromate by human erythrocytes.", "content": "Uptake of 51Cr-chromate by freshly collected human erythrocytes was inhibited by 90 mM sodium fluoride only in cells preincubated with fluoride. In vitro storage also increased the sensitivity of the cells: 90 mM NaF clearly inhibited the uptake of chromate in cells stored in vitro for 5 weeks. Gel chromatography revealed two forms of 51Cr within the cells, one of small molecular weight, which thin layer chromatography indicated was unchanged chromate; the other form was apparently bound to macromolecules such as hemoglobin.", "contents": "Influence of fluoride on the uptake of 51Cr-chromate by human erythrocytes. Uptake of 51Cr-chromate by freshly collected human erythrocytes was inhibited by 90 mM sodium fluoride only in cells preincubated with fluoride. In vitro storage also increased the sensitivity of the cells: 90 mM NaF clearly inhibited the uptake of chromate in cells stored in vitro for 5 weeks. Gel chromatography revealed two forms of 51Cr within the cells, one of small molecular weight, which thin layer chromatography indicated was unchanged chromate; the other form was apparently bound to macromolecules such as hemoglobin."}
{"id": "PMID:479877", "title": "Reactions of molybdenum-sulphur compounds with cyanide: chemical evolution and deactivation of molybdoenzymes.", "content": "Reactions of molybdenum-sulphur compounds with cyanide are reported which may be relevant to (1) the chemical evolution of molybdoenzymes and (2) deactivation of molybdoenzymes by cyanide. (1) With aqueous cyanide MoS2 gave thio-bridged complex anions [(Mo(CN)6)2(mu-S)]6- and [(Mo(CN)4(mu-S))2]6-. Under prebiotic conditions such complexes could have been formed similarly from molybdenite and may have been precursors of molybdoenzymes. (2) Only those compounds which contained terminal sulphur bound to molybdenum (i.e., Mo = S groups), viz. oxothiomolybdates and the complex [(Mo(mu-S)(S)(Et2NCS2))2], reacted with cyanide; thiocyanate was formed and the molybdenum underwent two-electron reduction. That the cyanolysable sulphur of xanthine oxidase reacts in the same way with cyanide suggests the presence of a Mo = S group which could be a structural feature of the enzyme or could have been formed by initial cyanolysis of a bound persulphide or cysteine residue.", "contents": "Reactions of molybdenum-sulphur compounds with cyanide: chemical evolution and deactivation of molybdoenzymes. Reactions of molybdenum-sulphur compounds with cyanide are reported which may be relevant to (1) the chemical evolution of molybdoenzymes and (2) deactivation of molybdoenzymes by cyanide. (1) With aqueous cyanide MoS2 gave thio-bridged complex anions [(Mo(CN)6)2(mu-S)]6- and [(Mo(CN)4(mu-S))2]6-. Under prebiotic conditions such complexes could have been formed similarly from molybdenite and may have been precursors of molybdoenzymes. (2) Only those compounds which contained terminal sulphur bound to molybdenum (i.e., Mo = S groups), viz. oxothiomolybdates and the complex [(Mo(mu-S)(S)(Et2NCS2))2], reacted with cyanide; thiocyanate was formed and the molybdenum underwent two-electron reduction. That the cyanolysable sulphur of xanthine oxidase reacts in the same way with cyanide suggests the presence of a Mo = S group which could be a structural feature of the enzyme or could have been formed by initial cyanolysis of a bound persulphide or cysteine residue."}
{"id": "PMID:479878", "title": "Intestinal metallothionein and the mutual antagonism between copper and zinc in the rat.", "content": "A study has been made of the mechanism of the mutual antagonism between copper and zinc in rats. Dietary zinc concentrations of up to 450 mg/kg had no effect on intestinal 64Cu absorption but 900 mg/kg caused a 40% reduction. This was associated with an increase in the mucosal uptake of 64Cu in the small intestine. This occurred mainly in the form of metallothionein and it appeared that copper displaced zinc from the protein after its synthesis had been induced by zinc. Ths intestinal absorption of 65Zn was decreased by 20% when the dietary copper intake was increased from 3 to 24 mg/kg. Further increases in copper intake to 300 mg/kg did not cause any additional decrease in 65Zn absorption or any change in the association of intestinal 65Zn with metallothionein. Concentrations of this protein in the intestinal mucosa were not influenced by dietary copper intake.", "contents": "Intestinal metallothionein and the mutual antagonism between copper and zinc in the rat. A study has been made of the mechanism of the mutual antagonism between copper and zinc in rats. Dietary zinc concentrations of up to 450 mg/kg had no effect on intestinal 64Cu absorption but 900 mg/kg caused a 40% reduction. This was associated with an increase in the mucosal uptake of 64Cu in the small intestine. This occurred mainly in the form of metallothionein and it appeared that copper displaced zinc from the protein after its synthesis had been induced by zinc. Ths intestinal absorption of 65Zn was decreased by 20% when the dietary copper intake was increased from 3 to 24 mg/kg. Further increases in copper intake to 300 mg/kg did not cause any additional decrease in 65Zn absorption or any change in the association of intestinal 65Zn with metallothionein. Concentrations of this protein in the intestinal mucosa were not influenced by dietary copper intake."}
{"id": "PMID:479897", "title": "Quadruple sectoranopia and sectorial optic atrophy: a syndrome of the distal anterior choroidal artery.", "content": "Loss of upper and lower homonymous sectors in the visual field, and wasting of corresponding sectors in the retinal nerve fibre layer, followed ligation of the distal part of the anterior choroidal artery in a patient with a meningioma of the velum interpositum. Clinical and radiological evidence indicated that the visual pathway was damaged within that part of the lateral geniculate body that is served by the anterior choroidal artery.", "contents": "Quadruple sectoranopia and sectorial optic atrophy: a syndrome of the distal anterior choroidal artery. Loss of upper and lower homonymous sectors in the visual field, and wasting of corresponding sectors in the retinal nerve fibre layer, followed ligation of the distal part of the anterior choroidal artery in a patient with a meningioma of the velum interpositum. Clinical and radiological evidence indicated that the visual pathway was damaged within that part of the lateral geniculate body that is served by the anterior choroidal artery."}
{"id": "PMID:479898", "title": "Immune complexes in myasthenia gravis.", "content": "Complement components were measured and immune complexes were sought in 75 patients with myasthenia gravis. Thirty-four per cent had decreased concentrations of complement component C4, and 29% had circulating immune complexes. The greatest immunological abnormalities were found in patients with mild disease which supports recent immunoelectronmicroscopic findings.", "contents": "Immune complexes in myasthenia gravis. Complement components were measured and immune complexes were sought in 75 patients with myasthenia gravis. Thirty-four per cent had decreased concentrations of complement component C4, and 29% had circulating immune complexes. The greatest immunological abnormalities were found in patients with mild disease which supports recent immunoelectronmicroscopic findings."}
{"id": "PMID:479899", "title": "Haemophilic neuromyopathy.", "content": "Twenty haemophilic patients were examined neurologically and found to have varying degrees of muscle atrophy. The pathogenesis of the atrophy was investigated by electromyography, serum muscle enzyme levels, and muscle biopsies. In most cases the EMG showed such abnormalities as reduced numbers of functioning motor units and \"myopathic\" motor unit potentials. Serum enzyme levels were frequently raised, and type 2 fibre atrophy was found in the three patients who underwent muscle biopsy. These findings suggest the presence of neuromyopathic involvement in haemophilic patients.", "contents": "Haemophilic neuromyopathy. Twenty haemophilic patients were examined neurologically and found to have varying degrees of muscle atrophy. The pathogenesis of the atrophy was investigated by electromyography, serum muscle enzyme levels, and muscle biopsies. In most cases the EMG showed such abnormalities as reduced numbers of functioning motor units and \"myopathic\" motor unit potentials. Serum enzyme levels were frequently raised, and type 2 fibre atrophy was found in the three patients who underwent muscle biopsy. These findings suggest the presence of neuromyopathic involvement in haemophilic patients."}
{"id": "PMID:479900", "title": "Pathophysiological mechanisms in cerebral palsy.", "content": "To investigate some of the pathophysiological mechanisms in cerebral palsy, surface electromyograms (EMG) were recorded from pairs of flexor/extensor muscles during both voluntary and passive flexion/extension of upper and lower limbs of 20 patients. Elbow, knee, or ankle joint angles were measured simultaneously, as well as the force required to flex/extend the limbs passively at frequencies of 0.1--1.0 Hz. In addition, single motor units were recorded from the first dorsal interosseous muscles of six of the patients. Almost all patients showed resistance to passive movements (hypertonia). This hypertonia did not necessarily impair voluntary flexion/extension movements if alternating EMG activity was maintained in at least one of the pairs of flexor/extensor muscles involved in the movement. In six severly involved patients, there was a complete breakdown in the reciprocal relationship between reciprocally acting pairs of flexor/extensor motoneurones, which resulted in synchronous activation (co-contractions) of flexor/extensor muscles during both voluntary and passive movements. In these patients the hyperactive segmental reflex added to the disabling effects of co-contractions during voluntary movements. Single motor units recorded from patients with dystonic movements were recruited with variable delays (2--10 s) and usually discharged intermittently at high frequencies (60--120/s). This abnormla motor unit discharge pattern may relate to pathology of the basal ganglia.", "contents": "Pathophysiological mechanisms in cerebral palsy. To investigate some of the pathophysiological mechanisms in cerebral palsy, surface electromyograms (EMG) were recorded from pairs of flexor/extensor muscles during both voluntary and passive flexion/extension of upper and lower limbs of 20 patients. Elbow, knee, or ankle joint angles were measured simultaneously, as well as the force required to flex/extend the limbs passively at frequencies of 0.1--1.0 Hz. In addition, single motor units were recorded from the first dorsal interosseous muscles of six of the patients. Almost all patients showed resistance to passive movements (hypertonia). This hypertonia did not necessarily impair voluntary flexion/extension movements if alternating EMG activity was maintained in at least one of the pairs of flexor/extensor muscles involved in the movement. In six severly involved patients, there was a complete breakdown in the reciprocal relationship between reciprocally acting pairs of flexor/extensor motoneurones, which resulted in synchronous activation (co-contractions) of flexor/extensor muscles during both voluntary and passive movements. In these patients the hyperactive segmental reflex added to the disabling effects of co-contractions during voluntary movements. Single motor units recorded from patients with dystonic movements were recruited with variable delays (2--10 s) and usually discharged intermittently at high frequencies (60--120/s). This abnormla motor unit discharge pattern may relate to pathology of the basal ganglia."}
{"id": "PMID:479901", "title": "Alexander's disease: case report including histopathological and electron microscopic features.", "content": "We report a case of Alexander's disease in a black South African boy of 9 years. The child presented with a grossly abnormal stooped posture, generalised weakness, and slurred nasal speech. Computerised tomography revealed diffuse low radio-density confined to the white matter, and enlarged ventricles. Cerebral biopsy confirmed the diagnosis. The histopathological and electron microscopic features are essentially the same as those described in previous reports. We note the presence of dense clumps of material within some of the Rosenthal fibres, and can find no reference in the literature describing this. Sural nerve biopsy was normal.", "contents": "Alexander's disease: case report including histopathological and electron microscopic features. We report a case of Alexander's disease in a black South African boy of 9 years. The child presented with a grossly abnormal stooped posture, generalised weakness, and slurred nasal speech. Computerised tomography revealed diffuse low radio-density confined to the white matter, and enlarged ventricles. Cerebral biopsy confirmed the diagnosis. The histopathological and electron microscopic features are essentially the same as those described in previous reports. We note the presence of dense clumps of material within some of the Rosenthal fibres, and can find no reference in the literature describing this. Sural nerve biopsy was normal."}
{"id": "PMID:479902", "title": "Relationship between presence of vasoconstrictor activity in cerebrospinal fluid and time after subarachnoid haemorrhage from rupture of cerebral arterial aneurysms.", "content": "The relationship between clinical condition and vasoconstrictor factors in cerebrospinal fluid was studied in 19 patients for up to six weeks after subarachnoid haemorrhage. Vasoconstrictor activity was assayed biologically. Sixteen of 19 patients improved as vasoconstrictor activity declined; this pattern was not significantly influenced by surgery. Serial angiography was performed on three patients and a qualitative relationship was shown between arterial dilatation, clinical improvement, and reduced pharmacological activity.", "contents": "Relationship between presence of vasoconstrictor activity in cerebrospinal fluid and time after subarachnoid haemorrhage from rupture of cerebral arterial aneurysms. The relationship between clinical condition and vasoconstrictor factors in cerebrospinal fluid was studied in 19 patients for up to six weeks after subarachnoid haemorrhage. Vasoconstrictor activity was assayed biologically. Sixteen of 19 patients improved as vasoconstrictor activity declined; this pattern was not significantly influenced by surgery. Serial angiography was performed on three patients and a qualitative relationship was shown between arterial dilatation, clinical improvement, and reduced pharmacological activity."}
{"id": "PMID:479903", "title": "Plasma and cerebrospinal fluid tryptophan in multiple sclerosis and degenerative diseases.", "content": "Tryptophan and competing neutral amino acid levels were found to be diminished in the plasma of patients with multiple sclerosis and degenerative diseases, the greatest decrease being of tryptophan. Cerebrospinal fluid tryptophan was decreased in multiple sclerosis and motor neurone disease, while leucine and valine were increased. These changes might lead to decreased synthesis of brain serotonin and brain proteins. The ratio between neutral amino acids and tryptophan might be used as an ancillary test in the screening of degenerative diseases.", "contents": "Plasma and cerebrospinal fluid tryptophan in multiple sclerosis and degenerative diseases. Tryptophan and competing neutral amino acid levels were found to be diminished in the plasma of patients with multiple sclerosis and degenerative diseases, the greatest decrease being of tryptophan. Cerebrospinal fluid tryptophan was decreased in multiple sclerosis and motor neurone disease, while leucine and valine were increased. These changes might lead to decreased synthesis of brain serotonin and brain proteins. The ratio between neutral amino acids and tryptophan might be used as an ancillary test in the screening of degenerative diseases."}
{"id": "PMID:479904", "title": "Spinal cord regeneration in rats made immunologically unresponsive to CNS antigens.", "content": "Seventy days after complete spinal cord transection, both treated and untreated rats showed evidence that some corticospinal axons had regenerated. Rats made immunologically unresponsive to CNS tissue showed no increase in corticospinal regeneration as measured by orthograde axoplasmic flow of tritated proline or retrograde axoplasmic labelling with horeseradish peroxidase. However, treated rats did demonstrate electrophysiological evidence of regeneration of long ascending sensory pathways. Tolerant animals additionally treated with cyclophosphamide showed corticospinal axonal regeneration by tritated proline transport and electrophysiological techniques and also showed electrophysiological evidence of ascending sensory tract regeneration.", "contents": "Spinal cord regeneration in rats made immunologically unresponsive to CNS antigens. Seventy days after complete spinal cord transection, both treated and untreated rats showed evidence that some corticospinal axons had regenerated. Rats made immunologically unresponsive to CNS tissue showed no increase in corticospinal regeneration as measured by orthograde axoplasmic flow of tritated proline or retrograde axoplasmic labelling with horeseradish peroxidase. However, treated rats did demonstrate electrophysiological evidence of regeneration of long ascending sensory pathways. Tolerant animals additionally treated with cyclophosphamide showed corticospinal axonal regeneration by tritated proline transport and electrophysiological techniques and also showed electrophysiological evidence of ascending sensory tract regeneration."}
{"id": "PMID:479905", "title": "Involvement of peripheral vasomotor fibres in carpal tunnel syndrome.", "content": "It is often assumed that large diameter nerve fibres are affected selectively in patients with compressive peripheral nerve lesions. However, in five of 13 patients with the carpal tunnel syndrome, plethysmography revealed that the normal digital vasoconstrictor response to inspiration was abolished in the index finger but preserved in the little finger of the affected hand. This indicates that the function of sympathetic efferent fibres destined for a median-innervated digit is sometimes disturbed also in patients with median nerve entrapment at the wrist.", "contents": "Involvement of peripheral vasomotor fibres in carpal tunnel syndrome. It is often assumed that large diameter nerve fibres are affected selectively in patients with compressive peripheral nerve lesions. However, in five of 13 patients with the carpal tunnel syndrome, plethysmography revealed that the normal digital vasoconstrictor response to inspiration was abolished in the index finger but preserved in the little finger of the affected hand. This indicates that the function of sympathetic efferent fibres destined for a median-innervated digit is sometimes disturbed also in patients with median nerve entrapment at the wrist."}
{"id": "PMID:479906", "title": "Cutaneous sensory function in diabetes mellitus.", "content": "That disorders of cutaneous sensation are common in diabetes mellitus can be substantiated by quantitative cutaneous sensory testing. Cutaneous sensory disturbances are not clearly related to clinical factors such as the type, treatment, or duration of diabetes, or ocular complications. Diabetics can be distinguished from nondiabetics on quantitative examination of skin sensation. Juvenile diabetics appear to have fewer cutaneous abnormalities than adults who develop the disease, but the juvenile diabetic is not spared. Disorders of cutaneous sensation may represent a fundamental abnormality of the nervous system in diabetes mellitus. While altered peripheral sensory mechanisms are likely, abnormality of central sensory processing is not excluded by the results of this study.", "contents": "Cutaneous sensory function in diabetes mellitus. That disorders of cutaneous sensation are common in diabetes mellitus can be substantiated by quantitative cutaneous sensory testing. Cutaneous sensory disturbances are not clearly related to clinical factors such as the type, treatment, or duration of diabetes, or ocular complications. Diabetics can be distinguished from nondiabetics on quantitative examination of skin sensation. Juvenile diabetics appear to have fewer cutaneous abnormalities than adults who develop the disease, but the juvenile diabetic is not spared. Disorders of cutaneous sensation may represent a fundamental abnormality of the nervous system in diabetes mellitus. While altered peripheral sensory mechanisms are likely, abnormality of central sensory processing is not excluded by the results of this study."}
{"id": "PMID:479907", "title": "Single fibre electromyography in central core disease.", "content": "Single fibre electromyography in the extensor digitorum communis muscle was studied in five patients with central core disease. The average number of muscle fibre action potentials belonging to the same motor unit was higher in patients than in healthy subjects of the same age. The increase in motor unit fibre density is consistent with increased terminal innervation ratio described in other papers about central core disease.", "contents": "Single fibre electromyography in central core disease. Single fibre electromyography in the extensor digitorum communis muscle was studied in five patients with central core disease. The average number of muscle fibre action potentials belonging to the same motor unit was higher in patients than in healthy subjects of the same age. The increase in motor unit fibre density is consistent with increased terminal innervation ratio described in other papers about central core disease."}
{"id": "PMID:479908", "title": "Cluster headache: trial of a combined histamine H1 and H2 antagonist treatment.", "content": "Fifteen patients with symptomatic cluster headache participated in a double-blind crossover trial of a combined histamine H1 and H2 antagonist treatment. The trial lasted six weeks. There was no significant improvement on active treatment as regards mean number of headache attacks per week, intensity, or duration of attacks. These results suggest that histamine does not play a significant role in the pathogenesis of cluster headache.", "contents": "Cluster headache: trial of a combined histamine H1 and H2 antagonist treatment. Fifteen patients with symptomatic cluster headache participated in a double-blind crossover trial of a combined histamine H1 and H2 antagonist treatment. The trial lasted six weeks. There was no significant improvement on active treatment as regards mean number of headache attacks per week, intensity, or duration of attacks. These results suggest that histamine does not play a significant role in the pathogenesis of cluster headache."}
{"id": "PMID:479909", "title": "Clonic hemifacial spasm from posterior fossa arteriovenous malformation.", "content": "A 21 year old woman presented with a two year history of right sided clonic hemifacial spasm. Investigations showed a large arteriovenous malformation on the right side of the posterior fossa and surgical exploration was undertaken. Early follow-up at six months showed a complete cure.", "contents": "Clonic hemifacial spasm from posterior fossa arteriovenous malformation. A 21 year old woman presented with a two year history of right sided clonic hemifacial spasm. Investigations showed a large arteriovenous malformation on the right side of the posterior fossa and surgical exploration was undertaken. Early follow-up at six months showed a complete cure."}
{"id": "PMID:479910", "title": "The significance of cerebral atrophy for the symptomatology of Parkinson's disease.", "content": "Hitherto published results on the impact of brain atrophy on the neurological and psychopathological sympion of this problem without risk of complications. We investigated 173 parkinsonian patients (89 men, 84 women) aged 37--83 years. Besides CT in all patients a standardized neurological and psychopathological investigation was carried out, including psychological tests, intelligence, personality structure, fine motor performance and visual reaction times. The investigations revealed pronounced correlations between CT-findings and more severe clinical symptomatology and a corresponding impairment in daily activities in the levodopa-treated and particularly in the untreated group, above all when there was a combination of cortical atrophy and ventricular enlargement. The same was true for fine motor performance and simple and complex reaction times. These functions were more impaired in patients with brain atrophy, irrespective of its localisation, whether cortical atrophy or ventricular enlargement. In contrast, no statistically significant relationship between the different parameters of brain atrophy and intelligence could be found. Considering cortical atrophy as a specific sign in Parkinson's disease -- our results are in favour of this assumption -- and ventricular enlargement more related to increasing age, parkinsonism is influenced by extranigral lesions inherent in the disease and dependent on increasing age.", "contents": "The significance of cerebral atrophy for the symptomatology of Parkinson's disease. Hitherto published results on the impact of brain atrophy on the neurological and psychopathological sympion of this problem without risk of complications. We investigated 173 parkinsonian patients (89 men, 84 women) aged 37--83 years. Besides CT in all patients a standardized neurological and psychopathological investigation was carried out, including psychological tests, intelligence, personality structure, fine motor performance and visual reaction times. The investigations revealed pronounced correlations between CT-findings and more severe clinical symptomatology and a corresponding impairment in daily activities in the levodopa-treated and particularly in the untreated group, above all when there was a combination of cortical atrophy and ventricular enlargement. The same was true for fine motor performance and simple and complex reaction times. These functions were more impaired in patients with brain atrophy, irrespective of its localisation, whether cortical atrophy or ventricular enlargement. In contrast, no statistically significant relationship between the different parameters of brain atrophy and intelligence could be found. Considering cortical atrophy as a specific sign in Parkinson's disease -- our results are in favour of this assumption -- and ventricular enlargement more related to increasing age, parkinsonism is influenced by extranigral lesions inherent in the disease and dependent on increasing age."}
{"id": "PMID:479911", "title": "Blood selenium in chronic spinal muscular atrophy.", "content": "The spinal muscular atrophies (SMA) of childhood comprise the second most common fatal recessive disease after cystic fibrosis, yet the nature of the biochemical defect causing the anterior horn cell degeneration is totally unknown. Recent reports of a cluster of adult motor neurone disease cases from a high seleniferous area in South Dakota have prompted the study of blood selenium in children with SMA in Australia. Eight children with chronic SMA were tested, in addition to 9 obligate heterozygote carriers of the gene. Blood selenium levels of patients and carriers did not differ significantly from that observed in controls. The mammalian effects of selenium toxicity are discussed.", "contents": "Blood selenium in chronic spinal muscular atrophy. The spinal muscular atrophies (SMA) of childhood comprise the second most common fatal recessive disease after cystic fibrosis, yet the nature of the biochemical defect causing the anterior horn cell degeneration is totally unknown. Recent reports of a cluster of adult motor neurone disease cases from a high seleniferous area in South Dakota have prompted the study of blood selenium in children with SMA in Australia. Eight children with chronic SMA were tested, in addition to 9 obligate heterozygote carriers of the gene. Blood selenium levels of patients and carriers did not differ significantly from that observed in controls. The mammalian effects of selenium toxicity are discussed."}
{"id": "PMID:479913", "title": "Clinical and electrophysiological studies of diphtheritic neuritis in Jordan.", "content": "Eleven patients with acute diphtheritic neuritis were studied clinically and electrophysiologically. Decreased conduction velocity and increased distal motor latency were present in most patients within two weeks of the onset of neurological symptoms. Velocities later fell to approximately 45% of mean normal values. Wasting with electrophysiological evidence of denervation was present in 3 patients. Nerve conduction studies had returned to normal within 3 months in 8 patients. There was a striking dissociation between the time course of the clinical and the neurophysiological abnormalities: early in the illness, peripheral nerve conduction was normal in some patients despite the presence of severe weakness, and later, the maximum electrophysiological abnormalities were sometimes found after clinical recovery had commenced. The nature and sequence of the clinical and electrophysiological changes are in accord with the known pattern and distribution of the pathological changes in the disease.", "contents": "Clinical and electrophysiological studies of diphtheritic neuritis in Jordan. Eleven patients with acute diphtheritic neuritis were studied clinically and electrophysiologically. Decreased conduction velocity and increased distal motor latency were present in most patients within two weeks of the onset of neurological symptoms. Velocities later fell to approximately 45% of mean normal values. Wasting with electrophysiological evidence of denervation was present in 3 patients. Nerve conduction studies had returned to normal within 3 months in 8 patients. There was a striking dissociation between the time course of the clinical and the neurophysiological abnormalities: early in the illness, peripheral nerve conduction was normal in some patients despite the presence of severe weakness, and later, the maximum electrophysiological abnormalities were sometimes found after clinical recovery had commenced. The nature and sequence of the clinical and electrophysiological changes are in accord with the known pattern and distribution of the pathological changes in the disease."}
{"id": "PMID:479914", "title": "Lack of correlation between spontaneous fasciculations and double discharges of voluntarily activated motor units.", "content": "We have studied the possible correlation between spontaneous fasciculations and double discharges or voluntarily activated motor units in myelopathies and other proximal neuropathies. Though both these phenomena are common in these diseases, they seem to have no correlation in individual muscles. It is concluded that spontaneous fasciculations and doubling of voluntarily activated motor unit potentials have different sites of origin or different mechanism. For example, if doubling reflects a disturbance of firing in the axon hillock, spontaneous fasciculations may originate in a more peripheral part of the motor axon.", "contents": "Lack of correlation between spontaneous fasciculations and double discharges of voluntarily activated motor units. We have studied the possible correlation between spontaneous fasciculations and double discharges or voluntarily activated motor units in myelopathies and other proximal neuropathies. Though both these phenomena are common in these diseases, they seem to have no correlation in individual muscles. It is concluded that spontaneous fasciculations and doubling of voluntarily activated motor unit potentials have different sites of origin or different mechanism. For example, if doubling reflects a disturbance of firing in the axon hillock, spontaneous fasciculations may originate in a more peripheral part of the motor axon."}
{"id": "PMID:479915", "title": "Observations on the effect of injection of lymphocytes from multiple sclerosis and Guillain-Barr\u00e9 syndrome in the rabbit eye model.", "content": "The effect of 10 X 10(5) peripheral blood lymphocytes (PBL) obtained from patients with multiple sclerosis (MS), Guillain-Barr\u00e9 syndrome (GBS) and healthy controls was studied after injection into the rabbit eye. Infiltration of inflammatory cells, strikingly localized to myelinated strips, was obtained with PBL from some of the MS patients and controls, indicating the non-specificity of the phenomenon. PBL from 2 GBS patients gave the strongest infiltration. No effect was obtained with 10 X 10(4) PBL or CSF lymphocytes from MS patients, indicating that the number of lymphocytes is an essential factor in the induction of lesions.", "contents": "Observations on the effect of injection of lymphocytes from multiple sclerosis and Guillain-Barr\u00e9 syndrome in the rabbit eye model. The effect of 10 X 10(5) peripheral blood lymphocytes (PBL) obtained from patients with multiple sclerosis (MS), Guillain-Barr\u00e9 syndrome (GBS) and healthy controls was studied after injection into the rabbit eye. Infiltration of inflammatory cells, strikingly localized to myelinated strips, was obtained with PBL from some of the MS patients and controls, indicating the non-specificity of the phenomenon. PBL from 2 GBS patients gave the strongest infiltration. No effect was obtained with 10 X 10(4) PBL or CSF lymphocytes from MS patients, indicating that the number of lymphocytes is an essential factor in the induction of lesions."}
{"id": "PMID:479916", "title": "Distal slowing of motor nerve conduction velocity in diabetic polyneuropathy.", "content": "Using the latencies of M response and F wave, motor nerve conduction was assessed along the entire course of the nerve from the spinal cord to the muscle in 102 diabetics and 74 control patients. In diabetics, latencies were increased and conduction velocity decreased over both proximal and distal segments. However, the latency ratio of the proximal to distal segment (F ratio) was slightly but significantly smaller in diabetics (mean +/- SD:0.93 +/- 0.14, 1.35 +/- 0.20, 1.09 +/- 0.19 and 1.02 +/- 0.19 for median, ulnar, tibial and peroneal nerves, respectively) than in control patients (1.05 +/- 0.09, 1.41 +/- 0.12, 1.17 +/- 0.13 and 1.08 +/- 0.12). These findings suggest that motor conduction abnormalities in diabetic polyneuropathy are diffuse over the total length of the nerve, but more intense in the distal than proximal segment. An additional finding in diabetics was that both proximal and distal segments were more frequently affected in the lower than in the upper extremities.", "contents": "Distal slowing of motor nerve conduction velocity in diabetic polyneuropathy. Using the latencies of M response and F wave, motor nerve conduction was assessed along the entire course of the nerve from the spinal cord to the muscle in 102 diabetics and 74 control patients. In diabetics, latencies were increased and conduction velocity decreased over both proximal and distal segments. However, the latency ratio of the proximal to distal segment (F ratio) was slightly but significantly smaller in diabetics (mean +/- SD:0.93 +/- 0.14, 1.35 +/- 0.20, 1.09 +/- 0.19 and 1.02 +/- 0.19 for median, ulnar, tibial and peroneal nerves, respectively) than in control patients (1.05 +/- 0.09, 1.41 +/- 0.12, 1.17 +/- 0.13 and 1.08 +/- 0.12). These findings suggest that motor conduction abnormalities in diabetic polyneuropathy are diffuse over the total length of the nerve, but more intense in the distal than proximal segment. An additional finding in diabetics was that both proximal and distal segments were more frequently affected in the lower than in the upper extremities."}
{"id": "PMID:479917", "title": "Studies of nuclear proteins in the skeletal muscle of the cardiomyopathic Syrian hamster.", "content": "Nonhistone nuclear proteins (NHNP) were isolated from the skeletal muscle of dystrophic hamsters and their respective paired controls. These proteins were extracted with phenol and fractionated by polyacrylamide gel electrophoresis. One-dimensional gel electrophoresis using either sodium-dodecylsulfate or isoelectrofocusing revealed quantitative changes between the two groups. An improved resolution using a two-dimensional system showed both quantitative and very limited qualitative differences. There are differences in proteins focusing at pH 5.0, molecular weight 55,000, in the dystrophic tissue. In addition proteins from dystrophic muscle focusing between pH 5.0 and 7.0, molecular weight 45,000, and proteins focusing between pH 7.0 and 9.0, molecular weights 35,000--45,000 and 60,000--70,000 were reduced as compared to the controls. There were no detectable differences in the electrophoretic patterns between the two groups of proteins derived from skeletal muscle homogenates. The differences in NHNP appear to be reflections of alterations in the nuclear composition of the dystrophic muscle cell. Some of these differences may represent changes secondary to the muscle disease. However, if NHNP interacting with DNA play a major role in the control of genetic expression, some of the manifestations of hamster dystrophy could be due to a different constitution of NHNP in the skeletal muscle.", "contents": "Studies of nuclear proteins in the skeletal muscle of the cardiomyopathic Syrian hamster. Nonhistone nuclear proteins (NHNP) were isolated from the skeletal muscle of dystrophic hamsters and their respective paired controls. These proteins were extracted with phenol and fractionated by polyacrylamide gel electrophoresis. One-dimensional gel electrophoresis using either sodium-dodecylsulfate or isoelectrofocusing revealed quantitative changes between the two groups. An improved resolution using a two-dimensional system showed both quantitative and very limited qualitative differences. There are differences in proteins focusing at pH 5.0, molecular weight 55,000, in the dystrophic tissue. In addition proteins from dystrophic muscle focusing between pH 5.0 and 7.0, molecular weight 45,000, and proteins focusing between pH 7.0 and 9.0, molecular weights 35,000--45,000 and 60,000--70,000 were reduced as compared to the controls. There were no detectable differences in the electrophoretic patterns between the two groups of proteins derived from skeletal muscle homogenates. The differences in NHNP appear to be reflections of alterations in the nuclear composition of the dystrophic muscle cell. Some of these differences may represent changes secondary to the muscle disease. However, if NHNP interacting with DNA play a major role in the control of genetic expression, some of the manifestations of hamster dystrophy could be due to a different constitution of NHNP in the skeletal muscle."}
{"id": "PMID:479918", "title": "Isolation and characterization of slow, depolarizing responses of cardiac ganglion neurons in the crab, Portunus sanguinolentus.", "content": "1. Tetrodotoxin-resistant, active responses to depolarization of the large cardiac ganglion cells were studied in semi-isolated preparations from the crab, Portunus sanguinolentus. Impulse activity was monitored with extracellular electrodes, simultaneous recordings from two or three large cells were made with intracellular electrodes, and current was passed via a bridge or second intracellular electrode. Preparations were continuously perfused with saline containing 3 x 10(-7) M tetrodotoxin (TTX). 2. About 20 min after introduction of TTX, small-cell impulses and resultant EPSPs in large cells cease, while rhythmic, spontaneous bursting of large cells continues. A pacemaker depolarization between bursts and slow depolarizations underlying the impulse bursts are prominent at this time. Shortly after, spontaneous burst rate slows, and at ca. 25 min, the ganglion becomes electrically quiescent. 3. In the quiescent, TTX-perfused ganglion, injection of depolarizing current into any one of the large cells results in active responses. At current strengths of sufficient intensity and duration (e.g., 20 nA, 20 ms; 5 nA, 500 ms) to depolarize a large cell by ca. 10 mV from resting potential (-53 mV, avg), the graded responses become regenerative and of constant form, provided the stimulation rate is less thna 0.15/s. Such responses have been termed \"driver potentials.\" At more rapid rates, thresholds are increased and responses reduced. 4. Driver potentials of anterior large cells reach peak amplitudes of ca. 20 mV (to -32 mV), have maximum rates of rise of 0.45 V/s and of fall of 0.2 V/s, and a duration of ca. 250 ms. They are followed by hyperpolarizing afterpotentials, a rapidly decaying one (1 s) to -58 mV, followed by a slowly decaying one (7.5 s), -55 mV. Responses of posterior large cells are smaller (16 mV) and slower; the site of active response may be at a distance from the soma. 5. The ability of elicit near-synchronous responses and the identity of amplitude and form of responses among anterior cells and of posterior cells, regardless of which cell receives depolarizing current, indicates that all cells undergo active responses and are stimulated by electrotonic spread of depolarization. 6. The responses involve a conductance increase since memses during a driver potential are much reduced. 7. Depolarization by steady current increases the absolute threshold, decreases the maximum depolarization of the peak, and slows rates of rise and fall. Hyperpolarization increases rates of rise and fall; the absolute value reached by the peak depolarization is unchanged. Hyperpolarization reduces the amplitude of the rapid after-potential relative to the displaced resting potential. 8. Hyperpolarizing current pulses imposed during the rise and peak of driver-potential responses are followed by redevelopment of a complete response. Sufficiently strong hyperpolarization can terminate a response. The current strength needed to terminate a response decreases the later during the response the pulse is given...", "contents": "Isolation and characterization of slow, depolarizing responses of cardiac ganglion neurons in the crab, Portunus sanguinolentus. 1. Tetrodotoxin-resistant, active responses to depolarization of the large cardiac ganglion cells were studied in semi-isolated preparations from the crab, Portunus sanguinolentus. Impulse activity was monitored with extracellular electrodes, simultaneous recordings from two or three large cells were made with intracellular electrodes, and current was passed via a bridge or second intracellular electrode. Preparations were continuously perfused with saline containing 3 x 10(-7) M tetrodotoxin (TTX). 2. About 20 min after introduction of TTX, small-cell impulses and resultant EPSPs in large cells cease, while rhythmic, spontaneous bursting of large cells continues. A pacemaker depolarization between bursts and slow depolarizations underlying the impulse bursts are prominent at this time. Shortly after, spontaneous burst rate slows, and at ca. 25 min, the ganglion becomes electrically quiescent. 3. In the quiescent, TTX-perfused ganglion, injection of depolarizing current into any one of the large cells results in active responses. At current strengths of sufficient intensity and duration (e.g., 20 nA, 20 ms; 5 nA, 500 ms) to depolarize a large cell by ca. 10 mV from resting potential (-53 mV, avg), the graded responses become regenerative and of constant form, provided the stimulation rate is less thna 0.15/s. Such responses have been termed \"driver potentials.\" At more rapid rates, thresholds are increased and responses reduced. 4. Driver potentials of anterior large cells reach peak amplitudes of ca. 20 mV (to -32 mV), have maximum rates of rise of 0.45 V/s and of fall of 0.2 V/s, and a duration of ca. 250 ms. They are followed by hyperpolarizing afterpotentials, a rapidly decaying one (1 s) to -58 mV, followed by a slowly decaying one (7.5 s), -55 mV. Responses of posterior large cells are smaller (16 mV) and slower; the site of active response may be at a distance from the soma. 5. The ability of elicit near-synchronous responses and the identity of amplitude and form of responses among anterior cells and of posterior cells, regardless of which cell receives depolarizing current, indicates that all cells undergo active responses and are stimulated by electrotonic spread of depolarization. 6. The responses involve a conductance increase since memses during a driver potential are much reduced. 7. Depolarization by steady current increases the absolute threshold, decreases the maximum depolarization of the peak, and slows rates of rise and fall. Hyperpolarization increases rates of rise and fall; the absolute value reached by the peak depolarization is unchanged. Hyperpolarization reduces the amplitude of the rapid after-potential relative to the displaced resting potential. 8. Hyperpolarizing current pulses imposed during the rise and peak of driver-potential responses are followed by redevelopment of a complete response. Sufficiently strong hyperpolarization can terminate a response. The current strength needed to terminate a response decreases the later during the response the pulse is given..."}
{"id": "PMID:479920", "title": "Cutaneous masking. II. Geometry of excitatory andinhibitory receptive fields of single units in somatosensory cortex of the cat.", "content": "1. The responses of single neurons in the primary somatosensory cortex of the cat to brief air-pulse stimuli were quantitatively examined. These controlled natural stimuli activated almost exclusively rapidly adapting hair units which, on systematic movement of the stimulus through the receptive field, gave unit-response profiles that showed the classical unimodal tent-shaped distribution. 2. Conditioning stimulus-induced inhibition of a response evoked by a fixed test stimulus was measured by systematically moving the conditioning stimulus through the receptive field. The spatial distribution of in-field inhibitory activity was unimodal and highly covariant with that of the conditioning excitation, the peak inhibition corresponding to the functional center of the excitatory receptive field. 3. Nearly one-half of the units studied evidenced inhibition extending beyond the excitatory receptive field, forming a \"surround\" inhibitory region; but these were usually restricted areas with rather weak inhibitory effects. 4. Time-course measuring revealed, on the average, inhibition effects measureable from 10 ms before to some 70 ms following conditioning stimulation, with peak inhibition delayed some 10--15 ms from the conditioning stimulus onset. We showed the backward inhibition, occurring with the test stimulus delivered before the onset of the conditioning stimulus, to be a property of the test response duration. Inhibition measured in the surround areas had essentially the same time course as the inhibition calculated from measurements made within the receptive fields. 5. The spatial and temporal profiles of the excitatory and inhibitory cortical unitary activity are thus very similar to the parametric features of psychophysical enhancement and masking. These findings suggest that the excitatory and inhibitory activities related to individual stimuli interact in multipoint stimulus paradigms so that simple unimodal composite profiles are synthesized.", "contents": "Cutaneous masking. II. Geometry of excitatory andinhibitory receptive fields of single units in somatosensory cortex of the cat. 1. The responses of single neurons in the primary somatosensory cortex of the cat to brief air-pulse stimuli were quantitatively examined. These controlled natural stimuli activated almost exclusively rapidly adapting hair units which, on systematic movement of the stimulus through the receptive field, gave unit-response profiles that showed the classical unimodal tent-shaped distribution. 2. Conditioning stimulus-induced inhibition of a response evoked by a fixed test stimulus was measured by systematically moving the conditioning stimulus through the receptive field. The spatial distribution of in-field inhibitory activity was unimodal and highly covariant with that of the conditioning excitation, the peak inhibition corresponding to the functional center of the excitatory receptive field. 3. Nearly one-half of the units studied evidenced inhibition extending beyond the excitatory receptive field, forming a \"surround\" inhibitory region; but these were usually restricted areas with rather weak inhibitory effects. 4. Time-course measuring revealed, on the average, inhibition effects measureable from 10 ms before to some 70 ms following conditioning stimulation, with peak inhibition delayed some 10--15 ms from the conditioning stimulus onset. We showed the backward inhibition, occurring with the test stimulus delivered before the onset of the conditioning stimulus, to be a property of the test response duration. Inhibition measured in the surround areas had essentially the same time course as the inhibition calculated from measurements made within the receptive fields. 5. The spatial and temporal profiles of the excitatory and inhibitory cortical unitary activity are thus very similar to the parametric features of psychophysical enhancement and masking. These findings suggest that the excitatory and inhibitory activities related to individual stimuli interact in multipoint stimulus paradigms so that simple unimodal composite profiles are synthesized."}
{"id": "PMID:479921", "title": "Forward masking of auditory nerve fiber responses.", "content": "1. Responses of single fibers were obtained from the auditory nerve of chinchillas. Tone-burst stimuli consisted of a masking stimulus followed by a probe stimulus. Forward masking of a fiber's response is defined as a reduction in the magnitude of the probe-evoked response caused by the addition of the masking stimulus. 2. The recovery of probe response magnitude as a function of the time interval between masker offset and probe onset (delta T) follows an exponential time course. A relationship between the time course or magnitude of poststimulus recovery and the characteristic frequency (CF) of a fiber was not detected. 3. The iso-forward masking contour near the threshold of the masking effect across masker frequencies approximates a fiber's frequency threshold curve (FTC). In other words, forward masking tuning curves are essentially the same as frequency threshold curves. 4. The frequency dependence of forward masking is compared to that of two-tone suppression. Tonal stimuli outside the boundaries of a fiber's FTC that produce two-tone suppression are ineffective forward maskers. Certain frequency/intensity combinations within the FTC may produce both suppression and forward masking and tones within the remaining area of the FTC produce no suppression but are effective forward maskers. 5. Both the time course and the magnitude of the forward masking effect are dependent on the discharge rate evoked by the masker regardless of the masker's absolute level or spectral content. An increase in masker-evoked excitation causes an increase in time constant and a greater reduction in probe response magnitude, rd. The function relating rd to masker level parallels the firing rate/masker level function up to 40 dB above response threshold. 6. A decrease in masker duration from 100 ms leads to a decrease in both rd and the time constant of recovery. There is no significant difference between the 100 and 200 ms duration conditions. 7. Forward masking in single fibers is related to the period of poststimulus recovery of spontaneous activity, a component of a fiber's response pattern to the masker, and this component is tentatively identified as a period of recovery from short-term adaptation.", "contents": "Forward masking of auditory nerve fiber responses. 1. Responses of single fibers were obtained from the auditory nerve of chinchillas. Tone-burst stimuli consisted of a masking stimulus followed by a probe stimulus. Forward masking of a fiber's response is defined as a reduction in the magnitude of the probe-evoked response caused by the addition of the masking stimulus. 2. The recovery of probe response magnitude as a function of the time interval between masker offset and probe onset (delta T) follows an exponential time course. A relationship between the time course or magnitude of poststimulus recovery and the characteristic frequency (CF) of a fiber was not detected. 3. The iso-forward masking contour near the threshold of the masking effect across masker frequencies approximates a fiber's frequency threshold curve (FTC). In other words, forward masking tuning curves are essentially the same as frequency threshold curves. 4. The frequency dependence of forward masking is compared to that of two-tone suppression. Tonal stimuli outside the boundaries of a fiber's FTC that produce two-tone suppression are ineffective forward maskers. Certain frequency/intensity combinations within the FTC may produce both suppression and forward masking and tones within the remaining area of the FTC produce no suppression but are effective forward maskers. 5. Both the time course and the magnitude of the forward masking effect are dependent on the discharge rate evoked by the masker regardless of the masker's absolute level or spectral content. An increase in masker-evoked excitation causes an increase in time constant and a greater reduction in probe response magnitude, rd. The function relating rd to masker level parallels the firing rate/masker level function up to 40 dB above response threshold. 6. A decrease in masker duration from 100 ms leads to a decrease in both rd and the time constant of recovery. There is no significant difference between the 100 and 200 ms duration conditions. 7. Forward masking in single fibers is related to the period of poststimulus recovery of spontaneous activity, a component of a fiber's response pattern to the masker, and this component is tentatively identified as a period of recovery from short-term adaptation."}
{"id": "PMID:479922", "title": "Studies on sensory neurons of the mouse with intracellular-recording and horseradish peroxidase-injection techniques.", "content": "1. Dorsal root ganglion (DRG) cells were dissected from the adult mouse with their peripheral nerves, and electrophysiological and morphological studies were performed. 2. The peripheral nerves were stimulateradish peroxidase (HRP) was injected into the cell body, and the size of stained cell body and axon, together with the state of myelination, were examined. 4. F-neurons, whose somatic spike is a tetrodotoxin (TTX)-sensitive Na spike, had a large or medium-sized cell body with a myelinated axon, and shoed a fast conduction velocity (A fibers). 5. A-neurons, whose somatic spike is a  TTX-resistant Na spike, had a small cell body with an unmyelinated axon, and showed a slow conduction velocity (C fibers). 6. Most H-neurons, whose somatic spike is a TTX-resistant combined Na-Ca spike, proved to have conduction velocity and morphological features similar to A-neurons, whereas the rest of them had features similar to F-neurons. 7. A roughly linear correlation was exhibited between each pair of cell body size, axon diameter, and conduction velcoity. 8. Spike conduction along axons to cell bodies were blocked in all neurons either by an elimination of Na ions from the medium or by an application of TTX. 9. The possibility of transmission of different information by different DRG cells and the developmental differentiation of sensory neurons are discussed.", "contents": "Studies on sensory neurons of the mouse with intracellular-recording and horseradish peroxidase-injection techniques. 1. Dorsal root ganglion (DRG) cells were dissected from the adult mouse with their peripheral nerves, and electrophysiological and morphological studies were performed. 2. The peripheral nerves were stimulateradish peroxidase (HRP) was injected into the cell body, and the size of stained cell body and axon, together with the state of myelination, were examined. 4. F-neurons, whose somatic spike is a tetrodotoxin (TTX)-sensitive Na spike, had a large or medium-sized cell body with a myelinated axon, and shoed a fast conduction velocity (A fibers). 5. A-neurons, whose somatic spike is a  TTX-resistant Na spike, had a small cell body with an unmyelinated axon, and showed a slow conduction velocity (C fibers). 6. Most H-neurons, whose somatic spike is a TTX-resistant combined Na-Ca spike, proved to have conduction velocity and morphological features similar to A-neurons, whereas the rest of them had features similar to F-neurons. 7. A roughly linear correlation was exhibited between each pair of cell body size, axon diameter, and conduction velcoity. 8. Spike conduction along axons to cell bodies were blocked in all neurons either by an elimination of Na ions from the medium or by an application of TTX. 9. The possibility of transmission of different information by different DRG cells and the developmental differentiation of sensory neurons are discussed."}
{"id": "PMID:479923", "title": "Different species of alpha motoneurons in same pool: further evidence from effects of inhibition on their firing rates.", "content": "1. Single units of the plantaris pool were isolated in ventral root filaments of decerebrate cats and their critical firing levels (CFLs) were determined. Motoneurons of similar size were compared in firing rate (FR) during repetitive stimulation of the plantaris nerve to establish control values and also during added stimulation of various inhibitory nerves (sural, hamstrings, or peroneal). 2. Criteria, based on maximal firing rate, were developed whereby certain pairs of units of similar size could be reliably classified into different types. 3. A second, independent set of criteria was formulated by which the same pair of units could be classified according to their responses to added inhibitory inputs. 4. The ability to distinguish motoneurons consistently by more than one set of criteria reinforces the evidence that different physiological types of units exist within a single motoneuron pool. 5. The findings indicate that different types of cells either receive different densities of input from certain inhibitory sources or that they react differentially to the same amounts of these inputs.", "contents": "Different species of alpha motoneurons in same pool: further evidence from effects of inhibition on their firing rates. 1. Single units of the plantaris pool were isolated in ventral root filaments of decerebrate cats and their critical firing levels (CFLs) were determined. Motoneurons of similar size were compared in firing rate (FR) during repetitive stimulation of the plantaris nerve to establish control values and also during added stimulation of various inhibitory nerves (sural, hamstrings, or peroneal). 2. Criteria, based on maximal firing rate, were developed whereby certain pairs of units of similar size could be reliably classified into different types. 3. A second, independent set of criteria was formulated by which the same pair of units could be classified according to their responses to added inhibitory inputs. 4. The ability to distinguish motoneurons consistently by more than one set of criteria reinforces the evidence that different physiological types of units exist within a single motoneuron pool. 5. The findings indicate that different types of cells either receive different densities of input from certain inhibitory sources or that they react differentially to the same amounts of these inputs."}
{"id": "PMID:479924", "title": "Stumbling corrective reaction: a phase-dependent compensatory reaction during locomotion.", "content": "1. Tactile stimuli to the paw consisting of a stick making contact or an air puff aimed at the dorsum were used to study the phasic influence of locomotor activity on the reflex pattern elicited in extensor and flexor muscles and on the induced compensatory movements in intact cats. The resulting movements and reflex pattern are called \"stumbling corrective reactions.\" 2. The basic reflex pattern and movements of the stumbling corrective reaction are: a) if the stimulus occurs during the swing phase, a short-latency activation of the flexor muscles, which induces an additional flexion of the limb lifting the paw over the obstacle; b) if the stimulus occurs during the support phase, an inhibition followed by an excitation of the extensor muscles, which neither increase nor decrease the extension. However, the stimulus evokes an increased flexor activity in the succeeding swing phase, which induces a brisker flexion. 3. Tactile stimuli to the proximal part of the limb or to the belly in front of the knee evoked the same type of phase-dependent compensatory reactions. Such reactions would presumably be beneficial for the animal to avoid high obstacles that impede movement. 4. A nonnoxious electrical stimulus (typically 2 mA; 1 ms) applied to the dorsum of the paw was used to study systematically the reflex pattern of the stumbling corrective reaction. Two pathways were defined to the knee flexor (semitendinosus). One early burst was evoked at about 10 ms and one later at about 25 ms after the stimulus. Short inhibitory pathways and longer excitatory pathways (20-50 ms) projected to the extensor nuclei. A short-latency (10 ms) excitatory pathway to the ankle extensor (lateral gastrocnemius) was also activated. 5. A painful electrical stimulus applied to the dorsum of the paw evoked large flexor responses during the whole step cycle. During the support phase the locomotion was disrupted as the supporting limb was withdrawn. 6. The results demonstrate that intact cats are able to compensate rapidly for unpredicted perturbations and that the reflex pattern and the induced corrective movements are adapted to the locomotor activity so that functionally meaningful movements are evoked in each phase of the step cycle. 7. The evoked reflexes and their modulation are consistent with those previously found in chronic spinal cats during walking and in paralyzed spinal cats performing \"fictive locomotion.\" It is suggested that the same spinal pathways are used, and that they are controlled by the spinal \"locomotor generator.\"", "contents": "Stumbling corrective reaction: a phase-dependent compensatory reaction during locomotion. 1. Tactile stimuli to the paw consisting of a stick making contact or an air puff aimed at the dorsum were used to study the phasic influence of locomotor activity on the reflex pattern elicited in extensor and flexor muscles and on the induced compensatory movements in intact cats. The resulting movements and reflex pattern are called \"stumbling corrective reactions.\" 2. The basic reflex pattern and movements of the stumbling corrective reaction are: a) if the stimulus occurs during the swing phase, a short-latency activation of the flexor muscles, which induces an additional flexion of the limb lifting the paw over the obstacle; b) if the stimulus occurs during the support phase, an inhibition followed by an excitation of the extensor muscles, which neither increase nor decrease the extension. However, the stimulus evokes an increased flexor activity in the succeeding swing phase, which induces a brisker flexion. 3. Tactile stimuli to the proximal part of the limb or to the belly in front of the knee evoked the same type of phase-dependent compensatory reactions. Such reactions would presumably be beneficial for the animal to avoid high obstacles that impede movement. 4. A nonnoxious electrical stimulus (typically 2 mA; 1 ms) applied to the dorsum of the paw was used to study systematically the reflex pattern of the stumbling corrective reaction. Two pathways were defined to the knee flexor (semitendinosus). One early burst was evoked at about 10 ms and one later at about 25 ms after the stimulus. Short inhibitory pathways and longer excitatory pathways (20-50 ms) projected to the extensor nuclei. A short-latency (10 ms) excitatory pathway to the ankle extensor (lateral gastrocnemius) was also activated. 5. A painful electrical stimulus applied to the dorsum of the paw evoked large flexor responses during the whole step cycle. During the support phase the locomotion was disrupted as the supporting limb was withdrawn. 6. The results demonstrate that intact cats are able to compensate rapidly for unpredicted perturbations and that the reflex pattern and the induced corrective movements are adapted to the locomotor activity so that functionally meaningful movements are evoked in each phase of the step cycle. 7. The evoked reflexes and their modulation are consistent with those previously found in chronic spinal cats during walking and in paralyzed spinal cats performing \"fictive locomotion.\" It is suggested that the same spinal pathways are used, and that they are controlled by the spinal \"locomotor generator.\""}
{"id": "PMID:479930", "title": "Intraspinal tumors in children. A review of 81 cases.", "content": "The authors have presented 81 children with intraspinal tumors, all less than 16 years of age. The clinical features indicated that gait disturbance, pain, and sphincter disturbance are the most prominent complaints. Reflex changes, paralysis, and sensory impairment are the most frequent physical findings. Cerebrospinal fluid protein was recorded in 47 patients and was abnormal in 34. Spine radiography was abnormal in 58% of the patients. Seventy of the patients had myelography, and it was diagnostic in 67. Surgery was performed on 79 patients, with a 2.5% operative mortality (30 days postsurgery). Of the 49 patients who are alive, 91.5% are able to walk. Mean follow-up period was 8.5 years. The authors document the benign course of the intramedullary astrocytomas and suggest an approach to their treatment. They encourage an aggressive approach to metastatic intraspinal tumors. The latrogenic origin of some epidermoid tumors is noted. The importance of early diagnosis and treatment of the intraspinal tumors in children is emphasized.", "contents": "Intraspinal tumors in children. A review of 81 cases. The authors have presented 81 children with intraspinal tumors, all less than 16 years of age. The clinical features indicated that gait disturbance, pain, and sphincter disturbance are the most prominent complaints. Reflex changes, paralysis, and sensory impairment are the most frequent physical findings. Cerebrospinal fluid protein was recorded in 47 patients and was abnormal in 34. Spine radiography was abnormal in 58% of the patients. Seventy of the patients had myelography, and it was diagnostic in 67. Surgery was performed on 79 patients, with a 2.5% operative mortality (30 days postsurgery). Of the 49 patients who are alive, 91.5% are able to walk. Mean follow-up period was 8.5 years. The authors document the benign course of the intramedullary astrocytomas and suggest an approach to their treatment. They encourage an aggressive approach to metastatic intraspinal tumors. The latrogenic origin of some epidermoid tumors is noted. The importance of early diagnosis and treatment of the intraspinal tumors in children is emphasized."}
{"id": "PMID:479931", "title": "Microsurgical treatment of intracranial vascular malformations.", "content": "In the period 1968 to 1978, 83 vascular malformations (AVM's) were treated by operation: 65 were excised and 18 were managed by interruption of afferent arteries. Three cryptic AVM's (two in the thalamus and one in the caudate nucleus) were discovered during exploration of hematomas in normotensive young women. All three of these small malformations were detected on computerized tomographic scans; none was apparent on preoperative angiograms. Two of the four deaths in this series occurred after excision of large, high-flow fistulas; the proposed cause was restoration of normal perfusion pressure in a chronically ischemic hemisphere rendered incapable of autoregulation. The addition of operative adjuncts, in particular, bipolar coagulation and operative microscopy, has extended traditional indications for operation to include favorably situated AVM's that have not bled and certain AVM's situated in critical regions of the brain.", "contents": "Microsurgical treatment of intracranial vascular malformations. In the period 1968 to 1978, 83 vascular malformations (AVM's) were treated by operation: 65 were excised and 18 were managed by interruption of afferent arteries. Three cryptic AVM's (two in the thalamus and one in the caudate nucleus) were discovered during exploration of hematomas in normotensive young women. All three of these small malformations were detected on computerized tomographic scans; none was apparent on preoperative angiograms. Two of the four deaths in this series occurred after excision of large, high-flow fistulas; the proposed cause was restoration of normal perfusion pressure in a chronically ischemic hemisphere rendered incapable of autoregulation. The addition of operative adjuncts, in particular, bipolar coagulation and operative microscopy, has extended traditional indications for operation to include favorably situated AVM's that have not bled and certain AVM's situated in critical regions of the brain."}
{"id": "PMID:479932", "title": "Superficial temporal-middle cerebral artery bypass. A detailed analysis of multiple pre- and postoperative angiograms in 40 consecutive patients.", "content": "Pre- and postoperative angiograms on 40 patients undergoing superficial temporal-middle cerebral artery (STA-MCA) bypass surgery have been examined in detail. Multiple postoperative angiograms have been obtained to evaluate the change in both the bypass circuit and the intracranial circulation over time. A reproducible system for evaluating the degree of intracranial vascular filling via the bypass is introduced. The study shows that the STA and its anastomotic branch increase in size over time, measured in months, in the majority of patients. This is paralleled by a progressive increase in the degree of intracranial vascular filling. These changes are proportional to the severity of the vascular disease before surgery. The pattern of preoperative collateral circulation may change over time following the addition of the bypass circuit. The progressive change over time suggests that a static analysis at one time may belie the true effect of the surgery. The change of collateral circulation with augmentation of blood supply to areas of the brain other than those affected by the recent ischemic event, means that a total cerebral evaluation including neuropsychological testing may be necessary for adequate evaluation of the effect of the bypass surgery.", "contents": "Superficial temporal-middle cerebral artery bypass. A detailed analysis of multiple pre- and postoperative angiograms in 40 consecutive patients. Pre- and postoperative angiograms on 40 patients undergoing superficial temporal-middle cerebral artery (STA-MCA) bypass surgery have been examined in detail. Multiple postoperative angiograms have been obtained to evaluate the change in both the bypass circuit and the intracranial circulation over time. A reproducible system for evaluating the degree of intracranial vascular filling via the bypass is introduced. The study shows that the STA and its anastomotic branch increase in size over time, measured in months, in the majority of patients. This is paralleled by a progressive increase in the degree of intracranial vascular filling. These changes are proportional to the severity of the vascular disease before surgery. The pattern of preoperative collateral circulation may change over time following the addition of the bypass circuit. The progressive change over time suggests that a static analysis at one time may belie the true effect of the surgery. The change of collateral circulation with augmentation of blood supply to areas of the brain other than those affected by the recent ischemic event, means that a total cerebral evaluation including neuropsychological testing may be necessary for adequate evaluation of the effect of the bypass surgery."}
{"id": "PMID:479933", "title": "Vasospasm assessed by angiography and computerized tomography.", "content": "In 44 consecutive cases of ruptured cerebral aneurysm, vasospasm was demonstrated pre- or postoperatively. These cases were examined by bilateral carotid angiography and computerized tomography (CT), and the relationship between the angiographically visualized distribution of vasospasm, the neurological symptoms, and infarction seen on CT was evaluated. Vasospasm occurred in only some intracranial portions of the cerbral arteries that were immersed in blood-stained cerebrospinal fluid. Angiographically, diffuse vasospasm extensively involving bilateral carotid systems indicated the gravest prognosis for patients. Vasospasm affecting one carotid system and the anterior cerebral arteries on the opposite side often produced permanent neurological deficits. On the contrary, when vasospasm was restricted to one carotid system or to bilateral anterior cerebral arteries, it was usually associated with temporary neurological symptoms; however, it always produced residual neurological symptoms if it extended to the ascending branches (M3) of the middle cerebral arteries. Computerized tomography definitely demonstrated a low-density area or infarction in the territory of the spastic arteries in 25 (71%) of 35 cases with vasospasm. A low-density area was always detected when vasospasm occurred in M3 segments.", "contents": "Vasospasm assessed by angiography and computerized tomography. In 44 consecutive cases of ruptured cerebral aneurysm, vasospasm was demonstrated pre- or postoperatively. These cases were examined by bilateral carotid angiography and computerized tomography (CT), and the relationship between the angiographically visualized distribution of vasospasm, the neurological symptoms, and infarction seen on CT was evaluated. Vasospasm occurred in only some intracranial portions of the cerbral arteries that were immersed in blood-stained cerebrospinal fluid. Angiographically, diffuse vasospasm extensively involving bilateral carotid systems indicated the gravest prognosis for patients. Vasospasm affecting one carotid system and the anterior cerebral arteries on the opposite side often produced permanent neurological deficits. On the contrary, when vasospasm was restricted to one carotid system or to bilateral anterior cerebral arteries, it was usually associated with temporary neurological symptoms; however, it always produced residual neurological symptoms if it extended to the ascending branches (M3) of the middle cerebral arteries. Computerized tomography definitely demonstrated a low-density area or infarction in the territory of the spastic arteries in 25 (71%) of 35 cases with vasospasm. A low-density area was always detected when vasospasm occurred in M3 segments."}
{"id": "PMID:479934", "title": "Localization in somatic sensory and motor areas of human cerebral cortex as determined by direct recording of evoked potentials and electrical stimulation.", "content": "This paper reports and illustrates in figurine style results obtained by electrical stimulation of the cortex in 20 patients and by recording of cortical evoked potentials (EPs) in 13 of these patients, whose surgery required wide exposure of the Rolandic or paracentral regions of the cortex. This study is unique in that cutaneous receptive fields related to specific cortical sites were defined by mechanical stimulation, as is done in animals, in contrast to electrical stimulation of peripheral nerves at fixed sites, as in scalp EP recordings. Observations were made on pre- and postcentral gyri, on the second somatic sensory-motor area, on the supplementary motor area, and on the supplementary sensory area. In two patients with phantom limb pain, the pain was elicited in one on stimulation of the postcentral arm area, and in the other on stimulation of the supplementary sensory leg area. Surgical removal of these areas had the immediate effect of abolishing the phantoms and the pain. Long-term follow-up review was not possible. In one patient with severe Parkinson's disease, stimulating currents subthreshold for the elicitation of movement resulted in disappearance of tremor and rigidity for short periods after stimulation of the precentral gyrus. The possible patterns of organization of the human pre- and postcentral areas are considered and compared with those of the chimpanzee and other primates. In patients in whom data from pre- and postcentral gyri were adequate, it appeared that the precentral face-arm boundary is situated 1 to 2 cm higher than the corresponding postcentral boundary.", "contents": "Localization in somatic sensory and motor areas of human cerebral cortex as determined by direct recording of evoked potentials and electrical stimulation. This paper reports and illustrates in figurine style results obtained by electrical stimulation of the cortex in 20 patients and by recording of cortical evoked potentials (EPs) in 13 of these patients, whose surgery required wide exposure of the Rolandic or paracentral regions of the cortex. This study is unique in that cutaneous receptive fields related to specific cortical sites were defined by mechanical stimulation, as is done in animals, in contrast to electrical stimulation of peripheral nerves at fixed sites, as in scalp EP recordings. Observations were made on pre- and postcentral gyri, on the second somatic sensory-motor area, on the supplementary motor area, and on the supplementary sensory area. In two patients with phantom limb pain, the pain was elicited in one on stimulation of the postcentral arm area, and in the other on stimulation of the supplementary sensory leg area. Surgical removal of these areas had the immediate effect of abolishing the phantoms and the pain. Long-term follow-up review was not possible. In one patient with severe Parkinson's disease, stimulating currents subthreshold for the elicitation of movement resulted in disappearance of tremor and rigidity for short periods after stimulation of the precentral gyrus. The possible patterns of organization of the human pre- and postcentral areas are considered and compared with those of the chimpanzee and other primates. In patients in whom data from pre- and postcentral gyri were adequate, it appeared that the precentral face-arm boundary is situated 1 to 2 cm higher than the corresponding postcentral boundary."}
{"id": "PMID:479935", "title": "Cerebrospinal fluid conductance and compliance of the craniospinal space in normal-pressure hydrocephalus. A comparison between two methods for measuring conductance to outflow.", "content": "Conductance to outflow of cerebrospinal fluid (CSF) has been measured by both a lumboventricular perfusion and a bolus injection method in 24 patients with normal-pressure hydrocephalus. One purpose was to investigate whether the less time-consuming technique of bolus, injection gave results comparable to the results obtained by the lumboventricular perfusion technique. There was a poor correlation between the results obtained by the two measurements of conductance to outflow of CSF. It is concluded that the bolus-injection technique cannot substitute for the lumboventricular perfusion test. Compliance of the CSF space was measured by the bolus injection. The presence of B-waves, recorded from long-term intraventricular pressure monitoring, could be correlated to the sum of conductance to outflow and compliance. The correlation offers a possible explanation of the nature of B-waves.", "contents": "Cerebrospinal fluid conductance and compliance of the craniospinal space in normal-pressure hydrocephalus. A comparison between two methods for measuring conductance to outflow. Conductance to outflow of cerebrospinal fluid (CSF) has been measured by both a lumboventricular perfusion and a bolus injection method in 24 patients with normal-pressure hydrocephalus. One purpose was to investigate whether the less time-consuming technique of bolus, injection gave results comparable to the results obtained by the lumboventricular perfusion technique. There was a poor correlation between the results obtained by the two measurements of conductance to outflow of CSF. It is concluded that the bolus-injection technique cannot substitute for the lumboventricular perfusion test. Compliance of the CSF space was measured by the bolus injection. The presence of B-waves, recorded from long-term intraventricular pressure monitoring, could be correlated to the sum of conductance to outflow and compliance. The correlation offers a possible explanation of the nature of B-waves."}
{"id": "PMID:479936", "title": "Effect of impounder contact area on experimental spinal cord injury.", "content": "The spinal cords of two groups of dogs were subjected to 500 gm-cm impacts using two different configurations of impounders. The impounders varied only in area of contact with the dural surface. All other parameters were kept constant. With one impounder all of the dogs injured were totally paraplegic. The impounder consistently producing total paraplegia straddled the entire width of the dorsal surface of the spinal cord in a manner corresponding to the posterior arch of a vertebra. The injury produced with this impounder is not only consistently reproducible but bears a closer resemblance to clinical injury such as anterior vertebral dislocation than that produced with a smaller impounder.", "contents": "Effect of impounder contact area on experimental spinal cord injury. The spinal cords of two groups of dogs were subjected to 500 gm-cm impacts using two different configurations of impounders. The impounders varied only in area of contact with the dural surface. All other parameters were kept constant. With one impounder all of the dogs injured were totally paraplegic. The impounder consistently producing total paraplegia straddled the entire width of the dorsal surface of the spinal cord in a manner corresponding to the posterior arch of a vertebra. The injury produced with this impounder is not only consistently reproducible but bears a closer resemblance to clinical injury such as anterior vertebral dislocation than that produced with a smaller impounder."}
{"id": "PMID:479937", "title": "Iatrogenic carotid-cavernous fistula following Fogarty catheter thromboendarterectomy. Case report.", "content": "A case of iatrogenic carotid-cavernous fistula secondary to a Fogarty catheter thrombectomy is presented. The literature and seven previously reported cases are reviewed.", "contents": "Iatrogenic carotid-cavernous fistula following Fogarty catheter thromboendarterectomy. Case report. A case of iatrogenic carotid-cavernous fistula secondary to a Fogarty catheter thrombectomy is presented. The literature and seven previously reported cases are reviewed."}
{"id": "PMID:479938", "title": "Detection of cryptic vascular malformations by computerized tomography. Report of two cases.", "content": "Two cases of cryptic vascular malformation that were not demonstrated by cerebral angiography were detected by computerized tomography. One of these patients had a cavernous angioma in the fourth ventricle with recurrent subarachnoid hemorrhages, and the other harbored a small arteriovenous malformation and intracerebral hematoma. The usefulness and limitations of computerized tomography in the identification of cryptic vascular malformations are discussed.", "contents": "Detection of cryptic vascular malformations by computerized tomography. Report of two cases. Two cases of cryptic vascular malformation that were not demonstrated by cerebral angiography were detected by computerized tomography. One of these patients had a cavernous angioma in the fourth ventricle with recurrent subarachnoid hemorrhages, and the other harbored a small arteriovenous malformation and intracerebral hematoma. The usefulness and limitations of computerized tomography in the identification of cryptic vascular malformations are discussed."}
{"id": "PMID:479939", "title": "Internuclear ophthalmoplegia following head injury. Case report.", "content": "The most common causes of internuclear ophthalmoplegia (INO) are multiple sclerosis and vascular disease of the brain stem. Rarer causes are tumor, Arnold-Chiari malformation, and syphilis. Myasthenia gravis has, on occasion, presented with ocular abnormalities indistinguishable from INO. A case is described of bilateral INO of brief duration following head trauma. There were no other brain-stem abnormalities. This brings to 11 the number of reported patients in whom head trauma precipitated this abnormality.", "contents": "Internuclear ophthalmoplegia following head injury. Case report. The most common causes of internuclear ophthalmoplegia (INO) are multiple sclerosis and vascular disease of the brain stem. Rarer causes are tumor, Arnold-Chiari malformation, and syphilis. Myasthenia gravis has, on occasion, presented with ocular abnormalities indistinguishable from INO. A case is described of bilateral INO of brief duration following head trauma. There were no other brain-stem abnormalities. This brings to 11 the number of reported patients in whom head trauma precipitated this abnormality."}
{"id": "PMID:479940", "title": "High cervical spinal cord compression by an enterogenous cyst. Case report.", "content": "A case of enterogenous cyst causing compression of the spinal cord at C-1 is presented. The clinical course and radiological and histological findings are discussed.", "contents": "High cervical spinal cord compression by an enterogenous cyst. Case report. A case of enterogenous cyst causing compression of the spinal cord at C-1 is presented. The clinical course and radiological and histological findings are discussed."}
{"id": "PMID:479941", "title": "Cerebellar ganglioglioma. Case report.", "content": "This report presents clinicopathological documentation of a nerve-cell tumor in the right cerebellar hemisphere in a young woman. The nomenclature and some other aspects of tumors containing neuronal cells are discussed.", "contents": "Cerebellar ganglioglioma. Case report. This report presents clinicopathological documentation of a nerve-cell tumor in the right cerebellar hemisphere in a young woman. The nomenclature and some other aspects of tumors containing neuronal cells are discussed."}
{"id": "PMID:479942", "title": "Xanthogranuloma of the third ventricle. Case report.", "content": "The case history of a patient with a xanthogranuloma of the third ventricle is presented. This type of lesion formed by desquamation of epithelium is usually noted as an incidental autopsy finding in the choroid plexus of the lateral ventricles. Total removal via a transcortical transventricular route led to complete recovery. These tumors should be suspected in the geriatric population. The computerized tomography findings and a review of the literature are included.", "contents": "Xanthogranuloma of the third ventricle. Case report. The case history of a patient with a xanthogranuloma of the third ventricle is presented. This type of lesion formed by desquamation of epithelium is usually noted as an incidental autopsy finding in the choroid plexus of the lateral ventricles. Total removal via a transcortical transventricular route led to complete recovery. These tumors should be suspected in the geriatric population. The computerized tomography findings and a review of the literature are included."}
{"id": "PMID:479943", "title": "Abnormal origin of the posterior inferior cerebellar artery. Case report.", "content": "Posterior inferior cerebellar arteries originating from the vertebral arteries between the axis and the atlas were demonstrated angiographically in a 12-year-old boy. The patient presented with tetraparesis of sudden onset after a gymnastic exercise. There was no recognizable trauma.", "contents": "Abnormal origin of the posterior inferior cerebellar artery. Case report. Posterior inferior cerebellar arteries originating from the vertebral arteries between the axis and the atlas were demonstrated angiographically in a 12-year-old boy. The patient presented with tetraparesis of sudden onset after a gymnastic exercise. There was no recognizable trauma."}
{"id": "PMID:479946", "title": "Blood phosphorus levels of laying hens at various time intervals after dosing with phosphoric acid.", "content": "Two experiments were conducted to study the pattern of change in serum inorganic phosphorus level in laying hens fed a diet containing 0.30% total phosphorus after dosing with a known amount of phosphorus. Serum phosphorus level of undosed hens was 2.5 mg/100 ml at oviposition in both experiments. After dosing with 100 mg of phosphorus in 10 ml of a phosphoric acid solution, serum phosphorus level increased sharply and reached a peak of about 7.0 mg/100 ml between 30 and 60 minutes in experiment 2. After the peak the elevated level of serum phosphorus decreased logarithmically until 5 to 6 hours after dosing and eventually decreased to 2.5 mg/100 ml which was the serum phosphorus level of undosed hens in experiment 1. The half-life of the elevated portion of serum phosphorus due to the dosing was about 100 minutes.", "contents": "Blood phosphorus levels of laying hens at various time intervals after dosing with phosphoric acid. Two experiments were conducted to study the pattern of change in serum inorganic phosphorus level in laying hens fed a diet containing 0.30% total phosphorus after dosing with a known amount of phosphorus. Serum phosphorus level of undosed hens was 2.5 mg/100 ml at oviposition in both experiments. After dosing with 100 mg of phosphorus in 10 ml of a phosphoric acid solution, serum phosphorus level increased sharply and reached a peak of about 7.0 mg/100 ml between 30 and 60 minutes in experiment 2. After the peak the elevated level of serum phosphorus decreased logarithmically until 5 to 6 hours after dosing and eventually decreased to 2.5 mg/100 ml which was the serum phosphorus level of undosed hens in experiment 1. The half-life of the elevated portion of serum phosphorus due to the dosing was about 100 minutes."}
{"id": "PMID:479947", "title": "Determination of a copper requirement to support gestation and lactation for the female albino rat.", "content": "Confirmed pregnant female albino rats were fed a purified diet containing 3, 6, 9 or 12 ppm copper throughout gestation and lactation in order to determine a copper requirement for one generation. A diet containing 6 ppm copper was found to meet the biochemical needs for rats during gestation, but the combined stress of gestation plus lactation raised the dietary requirement to 9 ppm copper. Evidence to support these conclusions included measurements of serum copper concentration, serum ceruloplasmin activity, liver iron concentration, milk copper concentration and pup liver copper concentration. The results of these studies suggests that the current National Research Council recommendation of 5 ppm does not maximize the copper status of first generation off-spring in rats.", "contents": "Determination of a copper requirement to support gestation and lactation for the female albino rat. Confirmed pregnant female albino rats were fed a purified diet containing 3, 6, 9 or 12 ppm copper throughout gestation and lactation in order to determine a copper requirement for one generation. A diet containing 6 ppm copper was found to meet the biochemical needs for rats during gestation, but the combined stress of gestation plus lactation raised the dietary requirement to 9 ppm copper. Evidence to support these conclusions included measurements of serum copper concentration, serum ceruloplasmin activity, liver iron concentration, milk copper concentration and pup liver copper concentration. The results of these studies suggests that the current National Research Council recommendation of 5 ppm does not maximize the copper status of first generation off-spring in rats."}
{"id": "PMID:479950", "title": "Transport and metabolism of pyridoxamine and pyridoxamine phosphate in the small intestine of the rat.", "content": "The vascularly perfused small intestine of the rat was utilized to study the absorption and metabolism of pyridoxamine (PM) and pyridoxamine-5'-phosphate (PMP), independent of other tissues including erythrocytes. [3H]PM or [3H]PMP was administered intralumenally with or without the addition of unlabeled PM, or PMP or inorganic phosphate. The percentage absorption of PM was 17.1 to 19.7% in 10 minutes and was unaffected by dosages from 0.02 to 200 mumole. The isotope from [3H]PMP at a physiological level (0.02 mumole) was absorbed at the same rate as that from [3H]PM, and the distribution of the 3H remaining in the lumen and in the intestinal tissue and perfusate indicated that hydrolytic removal of the phosphate was occurring extensively in the gut. The spectrum of labeled compounds isolated from the lumen, the perfusate and the mucosa clearly indicated that PMP, unhydrolyzed, can be absorbed slowly and converted to a number of vitamin B-6 forms in the intestinal mucosa. However, the results support the view that under normal physiological conditions the majority of dietary PMP is hydrolyzed to PM which seems to be absorbed by passive diffusion and transported to other organs and tissues via the blood stream.", "contents": "Transport and metabolism of pyridoxamine and pyridoxamine phosphate in the small intestine of the rat. The vascularly perfused small intestine of the rat was utilized to study the absorption and metabolism of pyridoxamine (PM) and pyridoxamine-5'-phosphate (PMP), independent of other tissues including erythrocytes. [3H]PM or [3H]PMP was administered intralumenally with or without the addition of unlabeled PM, or PMP or inorganic phosphate. The percentage absorption of PM was 17.1 to 19.7% in 10 minutes and was unaffected by dosages from 0.02 to 200 mumole. The isotope from [3H]PMP at a physiological level (0.02 mumole) was absorbed at the same rate as that from [3H]PM, and the distribution of the 3H remaining in the lumen and in the intestinal tissue and perfusate indicated that hydrolytic removal of the phosphate was occurring extensively in the gut. The spectrum of labeled compounds isolated from the lumen, the perfusate and the mucosa clearly indicated that PMP, unhydrolyzed, can be absorbed slowly and converted to a number of vitamin B-6 forms in the intestinal mucosa. However, the results support the view that under normal physiological conditions the majority of dietary PMP is hydrolyzed to PM which seems to be absorbed by passive diffusion and transported to other organs and tissues via the blood stream."}
{"id": "PMID:479952", "title": "Effects of fetal and early postnatal thiamin deficiency on avoidance learning in rats.", "content": "Thiamin deficiency was induced in two groups of young rats during two stages of growth: deficiency 1, from 8 days prepartum to 10 days postpartum or deficiency 2, from 1 to 18 days postpartum. The deficiency was reversed by thiamin injections for 5 days and return to a normal diet following which animals were tested at 35 days of age. Body weights at 21 and 36 days of age were not significantly reduced by the deficiency nor was brain weight affected. Activity levels were not significantly reduced by deficiency 1 but were reduced in deficiency 2. Both active and passive avoidance learning were significantly impaired in both deficiency 1 and 2, a finding which could not be attributed to alterations in pain sensitivity, motor ability or reduced activity levels. The results indicate that the developing brain is vulnerable to reduced thiamin intake and that the period of vulnerability may be different for activity and avoidance learning.", "contents": "Effects of fetal and early postnatal thiamin deficiency on avoidance learning in rats. Thiamin deficiency was induced in two groups of young rats during two stages of growth: deficiency 1, from 8 days prepartum to 10 days postpartum or deficiency 2, from 1 to 18 days postpartum. The deficiency was reversed by thiamin injections for 5 days and return to a normal diet following which animals were tested at 35 days of age. Body weights at 21 and 36 days of age were not significantly reduced by the deficiency nor was brain weight affected. Activity levels were not significantly reduced by deficiency 1 but were reduced in deficiency 2. Both active and passive avoidance learning were significantly impaired in both deficiency 1 and 2, a finding which could not be attributed to alterations in pain sensitivity, motor ability or reduced activity levels. The results indicate that the developing brain is vulnerable to reduced thiamin intake and that the period of vulnerability may be different for activity and avoidance learning."}
{"id": "PMID:479954", "title": "Long term pancreatic response to feeding heat damaged casein in rats.", "content": "Rats were fed a heat damaged casein (autoclaved 24 hours, 121 degrees, 2 atm) diet to determine the effect of poorly digested protein on pancreatic enzyme levels and response to a meal. After 10 days of feeding, the pancreas showed no signs of atrophy, however, chymotrypsin and amylase activities were lower in proportion to body weight. An estimation of secretion during the meal was similar or slightly lower in rats fed heated casein (HC) as compared to the casein diet (C), but a greater levels of enzyme activity was found in the intestinal contents of rats fed HC relative to control rats. These results suggest that the turnover of enzymes in the gut is reduced when a less digestible protein is fed, and that the endogenous pancreatic secretions and the dietary protein are not digested and absorbed as well.", "contents": "Long term pancreatic response to feeding heat damaged casein in rats. Rats were fed a heat damaged casein (autoclaved 24 hours, 121 degrees, 2 atm) diet to determine the effect of poorly digested protein on pancreatic enzyme levels and response to a meal. After 10 days of feeding, the pancreas showed no signs of atrophy, however, chymotrypsin and amylase activities were lower in proportion to body weight. An estimation of secretion during the meal was similar or slightly lower in rats fed heated casein (HC) as compared to the casein diet (C), but a greater levels of enzyme activity was found in the intestinal contents of rats fed HC relative to control rats. These results suggest that the turnover of enzymes in the gut is reduced when a less digestible protein is fed, and that the endogenous pancreatic secretions and the dietary protein are not digested and absorbed as well."}
{"id": "PMID:479957", "title": "Iron deficiency and its role in cadmium-induced fetal growth retardation.", "content": "The aim of this investigation was to establish the relationship of cadmium-induced fetal growth retardation in the mouse to iron deficiency. Pregnant mice were either fed a low iron diet or given 40 ppm cadmium in their drinking water. The effects of these factors on fetal weight and hematological values of the fetuses and dams were established and compared, both with each other and with appropriate controls. Both treatments caused maternal and fetal anemia, the fetuses being more severely affected. The anemic fetuses were also severely growth retarded. These changes, when caused by the iron deficient diet, could be completely prevented by either parenterally or orally administered iron supplements. When the changes were caused by cadmium in the drinking water they were only partially prevented by oral supplements. From these results it was concluded that iron deficiency in pregnancy causes not only anemia but also fetal growth retardation. Cadmium exposure in pregnancy, presumably by blocking intestinal absorption of iron, also causes anemia and hence fetal growth retardation.", "contents": "Iron deficiency and its role in cadmium-induced fetal growth retardation. The aim of this investigation was to establish the relationship of cadmium-induced fetal growth retardation in the mouse to iron deficiency. Pregnant mice were either fed a low iron diet or given 40 ppm cadmium in their drinking water. The effects of these factors on fetal weight and hematological values of the fetuses and dams were established and compared, both with each other and with appropriate controls. Both treatments caused maternal and fetal anemia, the fetuses being more severely affected. The anemic fetuses were also severely growth retarded. These changes, when caused by the iron deficient diet, could be completely prevented by either parenterally or orally administered iron supplements. When the changes were caused by cadmium in the drinking water they were only partially prevented by oral supplements. From these results it was concluded that iron deficiency in pregnancy causes not only anemia but also fetal growth retardation. Cadmium exposure in pregnancy, presumably by blocking intestinal absorption of iron, also causes anemia and hence fetal growth retardation."}
{"id": "PMID:479958", "title": "Cadmium-induced fetal growth retardation in mice and the effects of dietary supplements of zinc, copper, iron and selenium.", "content": "Many of the toxic effects of cadmium can be prevented by prior or simultaneous administration of either zinc, copper, iron or selenium. The aim of this investigation was to determine whether cadmium-induced fetal growth retardation in the mouse could be prevented, or reduced, by dietary supplementation with salts of these elements. Pregnant mice, receiving either distilled water to drink or distilled water containing 40 ppm cadmium, were fed, throughout pregnancy, a normal stock mouse diet supplemented with either zinc (200, 400 or 800 ppm), copper (30, 100 or 400 ppm), iron (200 or 1,000 ppm) or selenium (0.05, 2, 20 or 200 ppm). Also included was a control group which did not receive cadmium or dietary supplements. The animals were killed on the 19th day of pregnancy and the fetuses removed and weighed. The results showed that the iron supplemented diets gave partial protection against the cadmium-induced growth retardation; the other diets were ineffective.", "contents": "Cadmium-induced fetal growth retardation in mice and the effects of dietary supplements of zinc, copper, iron and selenium. Many of the toxic effects of cadmium can be prevented by prior or simultaneous administration of either zinc, copper, iron or selenium. The aim of this investigation was to determine whether cadmium-induced fetal growth retardation in the mouse could be prevented, or reduced, by dietary supplementation with salts of these elements. Pregnant mice, receiving either distilled water to drink or distilled water containing 40 ppm cadmium, were fed, throughout pregnancy, a normal stock mouse diet supplemented with either zinc (200, 400 or 800 ppm), copper (30, 100 or 400 ppm), iron (200 or 1,000 ppm) or selenium (0.05, 2, 20 or 200 ppm). Also included was a control group which did not receive cadmium or dietary supplements. The animals were killed on the 19th day of pregnancy and the fetuses removed and weighed. The results showed that the iron supplemented diets gave partial protection against the cadmium-induced growth retardation; the other diets were ineffective."}
{"id": "PMID:479964", "title": "Effects of restricted diet and intestinal flora on the life span of small intestine epithelial cells in mice.", "content": "Previous data have shown that the life span of small intestine epithelial cells in germ-free (GF) mice was 4.3 days, while that in conventional (CV) mice was 2.1 days, under ad libitum feeding. On the other hand, in the author's laboratory, it was also found that feeding conditions affected the cells' life span. That is, in CV mice the life span of the cells lengthened under restricted feeding (2.6 days), compared with under and libitum feeding (1.8 days). In the present experiment the life span of small intestine epithelial cells was investigated using radioautography, under controlled feeding (setting it equal to ad libitum feeding) and restricted feeding, in both CV and GF mice. Small intestine samples were taken from the middle part of duodenum, jejunum and ileum. Body weight changes, organ wet weights and intestine were also measured. In the lower part of the small intestine the effects of a restricted diet on epithelial cell life span prolongation appeared clearly in CV mice, but this effect was reduced in GF mice. This may be partly because the restricted group had slightly shorter villi in the case of GF mice.", "contents": "Effects of restricted diet and intestinal flora on the life span of small intestine epithelial cells in mice. Previous data have shown that the life span of small intestine epithelial cells in germ-free (GF) mice was 4.3 days, while that in conventional (CV) mice was 2.1 days, under ad libitum feeding. On the other hand, in the author's laboratory, it was also found that feeding conditions affected the cells' life span. That is, in CV mice the life span of the cells lengthened under restricted feeding (2.6 days), compared with under and libitum feeding (1.8 days). In the present experiment the life span of small intestine epithelial cells was investigated using radioautography, under controlled feeding (setting it equal to ad libitum feeding) and restricted feeding, in both CV and GF mice. Small intestine samples were taken from the middle part of duodenum, jejunum and ileum. Body weight changes, organ wet weights and intestine were also measured. In the lower part of the small intestine the effects of a restricted diet on epithelial cell life span prolongation appeared clearly in CV mice, but this effect was reduced in GF mice. This may be partly because the restricted group had slightly shorter villi in the case of GF mice."}
{"id": "PMID:479965", "title": "Minor components of reduced bovine kappa-casein.", "content": "Bovine kappa-casein reduced with 2-mercaptoethanol was fractionated on a DEAE-cellulose column to one nonadsorbed, five major adsorbed and two minor adsorbed fractions. The properties of the nonadsorbed and five major adsorbed fractions were reported in our previous paper (4). In this paper, the characteristics of two minor adsorbed fractions (P-X and P-Y) were reported. Gel electrophoretic patterns of these fractions were similar to kappa-casein. These fractions had the same amino acid composition and the same phosphorus content as the major components. P-X contained one residue each of N-acetylneuraminic acid, galactose and galactosamine, and P-Y two residues each. Furthermore, these fractions showed the stabilizing ability for alpha s1-casein in the presence of calcium ion. These results indicate that P-X and P-Y are minor components of reduced bovine kappa-casein.", "contents": "Minor components of reduced bovine kappa-casein. Bovine kappa-casein reduced with 2-mercaptoethanol was fractionated on a DEAE-cellulose column to one nonadsorbed, five major adsorbed and two minor adsorbed fractions. The properties of the nonadsorbed and five major adsorbed fractions were reported in our previous paper (4). In this paper, the characteristics of two minor adsorbed fractions (P-X and P-Y) were reported. Gel electrophoretic patterns of these fractions were similar to kappa-casein. These fractions had the same amino acid composition and the same phosphorus content as the major components. P-X contained one residue each of N-acetylneuraminic acid, galactose and galactosamine, and P-Y two residues each. Furthermore, these fractions showed the stabilizing ability for alpha s1-casein in the presence of calcium ion. These results indicate that P-X and P-Y are minor components of reduced bovine kappa-casein."}
{"id": "PMID:479966", "title": "Dynamic contributions of the components of binocular vergence.", "content": "Experimental results from step stimulation of the two major dynamic components in ocular vergence showed that while fusional covergence dominated a coordinated binocular vergence movement, accomodative convergence always contributes to the dynamic response. A quantitative analysis of component dynamics, both in isolation and during coordinated movements, indicates that these components are additive at least through a portion of the movement. Two alternative hypotheses are presented for the role of accommodative convergence during the period immediately following a binocular vergence movement.", "contents": "Dynamic contributions of the components of binocular vergence. Experimental results from step stimulation of the two major dynamic components in ocular vergence showed that while fusional covergence dominated a coordinated binocular vergence movement, accomodative convergence always contributes to the dynamic response. A quantitative analysis of component dynamics, both in isolation and during coordinated movements, indicates that these components are additive at least through a portion of the movement. Two alternative hypotheses are presented for the role of accommodative convergence during the period immediately following a binocular vergence movement."}
{"id": "PMID:479967", "title": "The Mandelbaum effect: evidence for an accommodative bias toward intermediate viewing distances.", "content": "Previous research has shown that ocular accommodation tends to correspond to an intermediate distance, the dark focus, in the absence of effective stimulation. The present experiments measured accommodative responses in the presence of two adequate, monocular stimuli superimposed optically at different distances. In Experiment I, observers attempted to maintain a matrix of letters in clear focus as a superimposed mesh screen was varied in distance. In experiment II, observers were instructed to focus the \"easier\" of two similar grating patterns that were presented over a range of distances with a constant separation of two diopters. The results of both experiments showed an accomodative response bias toward target distances near the observers' dark focus of accommodation. The implications of these findings for the theoretical resting state of accomodation and for practical problems of visual performance are discussed.", "contents": "The Mandelbaum effect: evidence for an accommodative bias toward intermediate viewing distances. Previous research has shown that ocular accommodation tends to correspond to an intermediate distance, the dark focus, in the absence of effective stimulation. The present experiments measured accommodative responses in the presence of two adequate, monocular stimuli superimposed optically at different distances. In Experiment I, observers attempted to maintain a matrix of letters in clear focus as a superimposed mesh screen was varied in distance. In experiment II, observers were instructed to focus the \"easier\" of two similar grating patterns that were presented over a range of distances with a constant separation of two diopters. The results of both experiments showed an accomodative response bias toward target distances near the observers' dark focus of accommodation. The implications of these findings for the theoretical resting state of accomodation and for practical problems of visual performance are discussed."}
{"id": "PMID:479968", "title": "Bandwidths of orientation channels in human vision.", "content": "The ability of observers to discriminate between stimuli differing in orientation was measured using low contrast, foveally viewed stimuli. Detection and discrimination performance were measured simultaneously. A model is presented which permits bandwidths of orientation-tuned mechanisms to be estimated from the data. In a group of five observers, half-amplitude bandwidths varied from 10 degrees to 20 degrees.", "contents": "Bandwidths of orientation channels in human vision. The ability of observers to discriminate between stimuli differing in orientation was measured using low contrast, foveally viewed stimuli. Detection and discrimination performance were measured simultaneously. A model is presented which permits bandwidths of orientation-tuned mechanisms to be estimated from the data. In a group of five observers, half-amplitude bandwidths varied from 10 degrees to 20 degrees."}
{"id": "PMID:479969", "title": "Flicker sensitivity measured with intermittent stimuli: I. Influence of the stimulus duration on the flicker threshold.", "content": "Flicker tresholds have been measured with intermittent stimuli as a function of the presentation time of the stimulus. The periods of flickering light were separated by 1-s dark intervals. Under these conditions the flicker threshold depends on the stimulus duration and on the number of cycles in the stimulus. The \"De Lange\" curves measured in this way can be described by a first-order high-pass filter in combination with a fourth-order low-pass filter.", "contents": "Flicker sensitivity measured with intermittent stimuli: I. Influence of the stimulus duration on the flicker threshold. Flicker tresholds have been measured with intermittent stimuli as a function of the presentation time of the stimulus. The periods of flickering light were separated by 1-s dark intervals. Under these conditions the flicker threshold depends on the stimulus duration and on the number of cycles in the stimulus. The \"De Lange\" curves measured in this way can be described by a first-order high-pass filter in combination with a fourth-order low-pass filter."}
{"id": "PMID:479970", "title": "Flicker sensitivity measured with intermittent stimuli: II. Comparison between temporal gradient detection and the \"De Lange\" curves.", "content": "The threshold gradient for perception of a linear change in luminance with time has been measured as a function of the stimulus duration. These data are compared with the \"De Lange\" curves representing the flicker sensitivity measured with intermittent presentation of the stimulus as a function of the stimulus duration. For this purpose it is necessary to take into account the fact that a linear luminance gradient, being a relatively low-frequency signal, is affected less by the low-pass filter supposedly situated in the visual system than by a sinusoidal signal (flicker) of the same duration.", "contents": "Flicker sensitivity measured with intermittent stimuli: II. Comparison between temporal gradient detection and the \"De Lange\" curves. The threshold gradient for perception of a linear change in luminance with time has been measured as a function of the stimulus duration. These data are compared with the \"De Lange\" curves representing the flicker sensitivity measured with intermittent presentation of the stimulus as a function of the stimulus duration. For this purpose it is necessary to take into account the fact that a linear luminance gradient, being a relatively low-frequency signal, is affected less by the low-pass filter supposedly situated in the visual system than by a sinusoidal signal (flicker) of the same duration."}
{"id": "PMID:479971", "title": "Study of eccentric fixation with secondary visual feedback.", "content": "Secondary visual feedback (2VFB) is a visual signal derived from continuous measurement of eye position and provides an extra artificial indication of the point of gaze. 2VFB may be eccentrically displaced and subjects are able to visually superimpose 2VFB onto a visual target signal and thus achieve and maintain eccentric fixation. Initial transient patterns of movement depend upon training but even naive subjects can achieve eccentric fixation within the first 40 s of such a task. Individual strategies and idiosyncratic patterns are exaggerations of normal control and fixational eye movements. The variance of maintained fixation increases with eccentricity and appears to be related to visual acuity as well as to precision of ocular motor control.", "contents": "Study of eccentric fixation with secondary visual feedback. Secondary visual feedback (2VFB) is a visual signal derived from continuous measurement of eye position and provides an extra artificial indication of the point of gaze. 2VFB may be eccentrically displaced and subjects are able to visually superimpose 2VFB onto a visual target signal and thus achieve and maintain eccentric fixation. Initial transient patterns of movement depend upon training but even naive subjects can achieve eccentric fixation within the first 40 s of such a task. Individual strategies and idiosyncratic patterns are exaggerations of normal control and fixational eye movements. The variance of maintained fixation increases with eccentricity and appears to be related to visual acuity as well as to precision of ocular motor control."}
{"id": "PMID:479972", "title": "Psychophysical evidence for global feature processing in visual texture discrimination.", "content": "By defining texture as a global feature attained by integration over the image domain, we show that texture discrimination can be predicted for a special class of visual textures (composed of paired dots) as a function of such global features. We derive a psychophysical law based on these global features and show that differences in the variance of orientation, but not of dipole length, result in texture discrimination.", "contents": "Psychophysical evidence for global feature processing in visual texture discrimination. By defining texture as a global feature attained by integration over the image domain, we show that texture discrimination can be predicted for a special class of visual textures (composed of paired dots) as a function of such global features. We derive a psychophysical law based on these global features and show that differences in the variance of orientation, but not of dipole length, result in texture discrimination."}
{"id": "PMID:479973", "title": "Hierarchical detection structures in vision.", "content": "A simple example shows that a hierarchy of visual cells can display different behavior for different stimuli. Stimuli with spatial characteristics similar to the receptive fields of a given level in the hierarchy can display summation behavior revealing the organization of that level; but other stimuli may show the organization of cells at other levels. On the other hand, such behavior may not actually occur in vision because the required nonlinearity between levels is very specific and may not be physiologically realistic.", "contents": "Hierarchical detection structures in vision. A simple example shows that a hierarchy of visual cells can display different behavior for different stimuli. Stimuli with spatial characteristics similar to the receptive fields of a given level in the hierarchy can display summation behavior revealing the organization of that level; but other stimuli may show the organization of cells at other levels. On the other hand, such behavior may not actually occur in vision because the required nonlinearity between levels is very specific and may not be physiologically realistic."}
{"id": "PMID:479982", "title": "The possible role of the fibroblast in granuloma-induced bone resorption in the rat.", "content": "Granulomas were produced over the calvaria of rats by injection of a mixture of turpentine and peanut oil. The granulomas induced localised areas of bone resorption and these resorptive lesions were examined at the fine structural level. A close association was found between osteoclasts and fibroblasts at resorbing surfaces. Furthermore, fibroblasts but not osteoclasts were observed to engulf and phagocytose bone collagen fibrils. It is suggested that the fibroblast either alone or in collaboration with the osteoclast is involved in the destruction of bone collagen during chronic inflammatory disorder of bone.", "contents": "The possible role of the fibroblast in granuloma-induced bone resorption in the rat. Granulomas were produced over the calvaria of rats by injection of a mixture of turpentine and peanut oil. The granulomas induced localised areas of bone resorption and these resorptive lesions were examined at the fine structural level. A close association was found between osteoclasts and fibroblasts at resorbing surfaces. Furthermore, fibroblasts but not osteoclasts were observed to engulf and phagocytose bone collagen fibrils. It is suggested that the fibroblast either alone or in collaboration with the osteoclast is involved in the destruction of bone collagen during chronic inflammatory disorder of bone."}
{"id": "PMID:479984", "title": "Ultimobranchial body cysts in the human foetal thyroid: pathological implications.", "content": "In a histological study of 28 human foetal thyroids, ultimobranchial body cysts were found in seven foetuses. Large oval cells were seen adjacent to or within the cysts. There is a morphological resemblance between the cells associated with the ultimobranchial cysts and those of medullary carcinoma of the thyroid gland. This similarity, in humans, substantiates the hypothesis that medullary carcinoma arises from the parafollicular cells, which themselves are known to differentiate from ultimobranchial body tissue.", "contents": "Ultimobranchial body cysts in the human foetal thyroid: pathological implications. In a histological study of 28 human foetal thyroids, ultimobranchial body cysts were found in seven foetuses. Large oval cells were seen adjacent to or within the cysts. There is a morphological resemblance between the cells associated with the ultimobranchial cysts and those of medullary carcinoma of the thyroid gland. This similarity, in humans, substantiates the hypothesis that medullary carcinoma arises from the parafollicular cells, which themselves are known to differentiate from ultimobranchial body tissue."}
{"id": "PMID:479985", "title": "An experimental assessment of macroscopic enzyme techniques for the autopsy demonstration of myocardial infarction.", "content": "Experimental cardiac infarction produced in dogs by coronary artery ligation has been used to investigate the value of macroscopic histochemical enzyme loss in the diagnosis of myocardial infarction at post-mortem. Creative phosphokinase and non-specific dehydrogenase methods gave the best results but became positive only 5-6 hr after infarction. It is concluded that the method is of limited value in autopsy practice.", "contents": "An experimental assessment of macroscopic enzyme techniques for the autopsy demonstration of myocardial infarction. Experimental cardiac infarction produced in dogs by coronary artery ligation has been used to investigate the value of macroscopic histochemical enzyme loss in the diagnosis of myocardial infarction at post-mortem. Creative phosphokinase and non-specific dehydrogenase methods gave the best results but became positive only 5-6 hr after infarction. It is concluded that the method is of limited value in autopsy practice."}
{"id": "PMID:479988", "title": "Chronic diarrhea of childhood and the misuse of elimination diets.", "content": "A prospective study was undertaken of 108 children referred for outpatient evaluation of chronic recurrent diarrhea. The majority of the children did not have a serious underlying disorder. Elimination diets (milk free, egg free, wheat free) were widely prescribed for the treatment of chronic diarrhea and were given for longer intervals than originally recommended. Elimination diets sometimes resulted in inadequate caloric intake and failure to thrive. Wheat (gluten)-free diets were prescribed for over one month in 59% of children without a specific diagnosis being made. These findings indicate that elimination diets are frequently misused, and prolonged adherence to elimination diets may result in nutritional damage. The indiscriminate use of wheat-free diets for the treatment of chronic diarrhea may be masking the diagnosis of celiac disease and may account for the low incidence of this disorder in the United States.", "contents": "Chronic diarrhea of childhood and the misuse of elimination diets. A prospective study was undertaken of 108 children referred for outpatient evaluation of chronic recurrent diarrhea. The majority of the children did not have a serious underlying disorder. Elimination diets (milk free, egg free, wheat free) were widely prescribed for the treatment of chronic diarrhea and were given for longer intervals than originally recommended. Elimination diets sometimes resulted in inadequate caloric intake and failure to thrive. Wheat (gluten)-free diets were prescribed for over one month in 59% of children without a specific diagnosis being made. These findings indicate that elimination diets are frequently misused, and prolonged adherence to elimination diets may result in nutritional damage. The indiscriminate use of wheat-free diets for the treatment of chronic diarrhea may be masking the diagnosis of celiac disease and may account for the low incidence of this disorder in the United States."}
{"id": "PMID:479989", "title": "Late metabolic acidosis: a reassessment of the definition.", "content": "The term \"late metabolic acidosis\" is generally used to define a population of apparently healthy LBW infants who fail to grow and have a base deficit in excess of 5 mEq/l (CO2TOT less than 21 mM). A relationship between hypobasemia and the lack of appropriate growth was postulated. This conclusion was reached, however, in the absence of adequate information regarding the distribution of acid-base variables in healthy LBW infants. The results of this study demonstrate that the CO2TOT of LBW infants (n = 114) rises between birth and three weeks of life from a mean of 18.6 to 20.3 mM. The frequency distribution of CO2TOT values did not show any significant deviations from normality, and 2 SD included values as low as 14.5 mM. No difference in the rate of growth was detected between \"hypobasemic\" infants given a solution of bicarbonate calculated to bring their blood CO2TOT to greater than 21 mM and those given similar amounts of isotonic saline solution. The ability of the LBW infants to excrete an ammonium chloride load was not related to their acid-base status and was comparable to that of term infants. It is apparent that the definition of late metabolic acidosis needs to be reconsidered.", "contents": "Late metabolic acidosis: a reassessment of the definition. The term \"late metabolic acidosis\" is generally used to define a population of apparently healthy LBW infants who fail to grow and have a base deficit in excess of 5 mEq/l (CO2TOT less than 21 mM). A relationship between hypobasemia and the lack of appropriate growth was postulated. This conclusion was reached, however, in the absence of adequate information regarding the distribution of acid-base variables in healthy LBW infants. The results of this study demonstrate that the CO2TOT of LBW infants (n = 114) rises between birth and three weeks of life from a mean of 18.6 to 20.3 mM. The frequency distribution of CO2TOT values did not show any significant deviations from normality, and 2 SD included values as low as 14.5 mM. No difference in the rate of growth was detected between \"hypobasemic\" infants given a solution of bicarbonate calculated to bring their blood CO2TOT to greater than 21 mM and those given similar amounts of isotonic saline solution. The ability of the LBW infants to excrete an ammonium chloride load was not related to their acid-base status and was comparable to that of term infants. It is apparent that the definition of late metabolic acidosis needs to be reconsidered."}
{"id": "PMID:479990", "title": "Thymus size and its relationship to the respiratory distress syndrome.", "content": "Thymic size can be affected by both exogenous and endogenous glucocorticoids. The risk of respiratory distress syndrome is reduced after maternal steroid administration. To find whether fetal lung maturity correlates with size of the thymus, the cardiothymic:thoracic ratio was measured in 167 newborn infants with and without RDS. Mean CT/T was significantly greater (0.40 vs 0.35; P less than 0.001) in those babies with RDS. This relation was independent of gestational age, although an increase in CT/T with advancing gestational age was shown. Prepartum maternal steroid administration did not result in significant involution of the cardiothymic shadow when compared with control infants with and without RDS. The CT/T may be of use in predicting which premature babies are more likely to develop RDS.", "contents": "Thymus size and its relationship to the respiratory distress syndrome. Thymic size can be affected by both exogenous and endogenous glucocorticoids. The risk of respiratory distress syndrome is reduced after maternal steroid administration. To find whether fetal lung maturity correlates with size of the thymus, the cardiothymic:thoracic ratio was measured in 167 newborn infants with and without RDS. Mean CT/T was significantly greater (0.40 vs 0.35; P less than 0.001) in those babies with RDS. This relation was independent of gestational age, although an increase in CT/T with advancing gestational age was shown. Prepartum maternal steroid administration did not result in significant involution of the cardiothymic shadow when compared with control infants with and without RDS. The CT/T may be of use in predicting which premature babies are more likely to develop RDS."}
{"id": "PMID:479993", "title": "A study of attitudes and support systems of inner city adolescent mothers.", "content": "Because the simultaneous demands of adolescence and motherhood could result in special infant rearing attitudes and concerns, a prospective pilot study of inner city adolescent mothers was undertaken. A sample of primiparous adolescent mothers was compared with primiparous and multiparous nonadolescent mothers. Data were collected by home interviews at two weeks and three months postpartum. The major hypothesis, that the simultaneous occurrence of adolescence and motherhood would result in special infant rearing attitudes, concerns, and support systems which interfered with mothering, was not fully supported by our findings. Few of the adolescent mothers were married (8%) but of those not married, most (95%) lived in an extended family. Adolescent mothers, when compared with older mothers, were more likely to seek medical advice from their mothers as opposed to health professionals and were more insecure in their maternal self-image if caretaking was shared. Attitudinal differences around feeding were seen, but attitudes about spoiling, perception of the baby's behavior, discipline, caretaking, and enjoyment were the same among groups. Adolescent mothers and their infants provide physicians and other child health professional with a special pediatric family. The differences in available support systems and maternal self-image suggest areas for further investigation.", "contents": "A study of attitudes and support systems of inner city adolescent mothers. Because the simultaneous demands of adolescence and motherhood could result in special infant rearing attitudes and concerns, a prospective pilot study of inner city adolescent mothers was undertaken. A sample of primiparous adolescent mothers was compared with primiparous and multiparous nonadolescent mothers. Data were collected by home interviews at two weeks and three months postpartum. The major hypothesis, that the simultaneous occurrence of adolescence and motherhood would result in special infant rearing attitudes, concerns, and support systems which interfered with mothering, was not fully supported by our findings. Few of the adolescent mothers were married (8%) but of those not married, most (95%) lived in an extended family. Adolescent mothers, when compared with older mothers, were more likely to seek medical advice from their mothers as opposed to health professionals and were more insecure in their maternal self-image if caretaking was shared. Attitudinal differences around feeding were seen, but attitudes about spoiling, perception of the baby's behavior, discipline, caretaking, and enjoyment were the same among groups. Adolescent mothers and their infants provide physicians and other child health professional with a special pediatric family. The differences in available support systems and maternal self-image suggest areas for further investigation."}
{"id": "PMID:479994", "title": "A longitudinal study of body fatness in childhood and adolescence.", "content": "Data from the United States Health Examination Surveys were analyzed to determine changes in body fatness between childhood and adolescence. A national probability sample (2,177 children) was examined in both Cycle II (6 to 11 years) and Cycle III (12 to 17 years) of these surveys and comprised the study cohort. The interval between examinations was three or four years. Adiposity was measured as skinfold thickness and correlations between childhood and adolescent adiposity were explored. High rank-order correlations were found between the two examinations for each race-sex group (P less than 0.001). The relationship between childhood and adolescent fatness was independent of stature, skeletal and sexual maturation, and economic status. Childhood fatness was the most predictive factor for adolescent fatness. This strong relationship indicates that the potentially obese adolescent can be identified during childhood.", "contents": "A longitudinal study of body fatness in childhood and adolescence. Data from the United States Health Examination Surveys were analyzed to determine changes in body fatness between childhood and adolescence. A national probability sample (2,177 children) was examined in both Cycle II (6 to 11 years) and Cycle III (12 to 17 years) of these surveys and comprised the study cohort. The interval between examinations was three or four years. Adiposity was measured as skinfold thickness and correlations between childhood and adolescent adiposity were explored. High rank-order correlations were found between the two examinations for each race-sex group (P less than 0.001). The relationship between childhood and adolescent fatness was independent of stature, skeletal and sexual maturation, and economic status. Childhood fatness was the most predictive factor for adolescent fatness. This strong relationship indicates that the potentially obese adolescent can be identified during childhood."}
{"id": "PMID:479997", "title": "Ventilatory functions of normal children and young adults--Mexican-American, white, and black. I. Spirometry.", "content": "This is a study of the ventilatory functions of 1,805 normal Mexican-American, white, and black students of six public schools in Houston, Texas, with ages ranging from 7 to 20. A roll-seal piston type spirometer was used. The best performed forced vital capacity curve of each student was selected by the computer program from which the following measurements were extracted: FVC, FEV1, FEV1/FVC ratio, PEFR, and MMEF. Each student also had the peak expiratory flow rate measured by the Wright peak flowmeter to establish normal values with this instrument. Significant differences of lung volume and flow rate exist among the three races, and between male and female subjects. Prediction equations and prediction curves for each race and sex are presented. The results of the present study are compared with those of previously published works.", "contents": "Ventilatory functions of normal children and young adults--Mexican-American, white, and black. I. Spirometry. This is a study of the ventilatory functions of 1,805 normal Mexican-American, white, and black students of six public schools in Houston, Texas, with ages ranging from 7 to 20. A roll-seal piston type spirometer was used. The best performed forced vital capacity curve of each student was selected by the computer program from which the following measurements were extracted: FVC, FEV1, FEV1/FVC ratio, PEFR, and MMEF. Each student also had the peak expiratory flow rate measured by the Wright peak flowmeter to establish normal values with this instrument. Significant differences of lung volume and flow rate exist among the three races, and between male and female subjects. Prediction equations and prediction curves for each race and sex are presented. The results of the present study are compared with those of previously published works."}
{"id": "PMID:480000", "title": "Separation experiences: a new look at an old topic.", "content": "Research findings on the effects of separation are reviewed. Children of working mothers develop as well as those whose mothers remain at home. The effects of day care for very young children depend very much on the quality of care provided. Admission to hospital is stressful for preschool children through separation from the family, lack of opportunity to form new attachments, a strange environment, and disturbed parent-child relationships on return home; recurrent hospital admissions may have long-term sequelae, especially in children under chronic stress. An institutional upbringing need not impair cognitive development, but multiple rotating caretakers are likely to interfere with social development. Broken homes are associated with psychiatric disorder only in so far as they reflect family discord and disharmony. Bereavement is most likely to lead to prolonged grief reactions in older children and adolescents. Forced separations of mother and infant in the neonatal period may damage parent-child relationships. Many mechanisms are involved in these different experiences, and in only a few is the separation itself the main stress.", "contents": "Separation experiences: a new look at an old topic. Research findings on the effects of separation are reviewed. Children of working mothers develop as well as those whose mothers remain at home. The effects of day care for very young children depend very much on the quality of care provided. Admission to hospital is stressful for preschool children through separation from the family, lack of opportunity to form new attachments, a strange environment, and disturbed parent-child relationships on return home; recurrent hospital admissions may have long-term sequelae, especially in children under chronic stress. An institutional upbringing need not impair cognitive development, but multiple rotating caretakers are likely to interfere with social development. Broken homes are associated with psychiatric disorder only in so far as they reflect family discord and disharmony. Bereavement is most likely to lead to prolonged grief reactions in older children and adolescents. Forced separations of mother and infant in the neonatal period may damage parent-child relationships. Many mechanisms are involved in these different experiences, and in only a few is the separation itself the main stress."}
{"id": "PMID:480009", "title": "Lung function in children following repair of tracheoesophageal fistula.", "content": "Lung function and bronchial reactivity by methacholine challenge were studied in 24 patients seven to 18 years after repair of type I tracheoesophageal fistula. Only one patient had no abnormalities of the variables tested. Thirteen patients had obstructive airways disease, five had a restrictive defect, and 15 had a positive methacholine challenge. It is postulated that continuing subclinical aspiration of esophageal contents causes lung damage and renders the airways hyperreactive.", "contents": "Lung function in children following repair of tracheoesophageal fistula. Lung function and bronchial reactivity by methacholine challenge were studied in 24 patients seven to 18 years after repair of type I tracheoesophageal fistula. Only one patient had no abnormalities of the variables tested. Thirteen patients had obstructive airways disease, five had a restrictive defect, and 15 had a positive methacholine challenge. It is postulated that continuing subclinical aspiration of esophageal contents causes lung damage and renders the airways hyperreactive."}
{"id": "PMID:480010", "title": "Chlamydial infection of mothers and their infants.", "content": "In 340 women, cultured prospectively during their pregnancies, the rate of infection with Chlamydia trachomatis was 8.8%. The women with positive cultures tended to be younger and more often single and black than their counterparts with negative cultures. There were no statistically significant clinical differences between the two groups. Eighteen children born to Chlamydia culture-positive women and 16 born to negative women were followed for nine months to examine the potential effects of maternal infection on infant growth, development, and illness. Eleven of 18 study patients had culture or tear antibody evidence of Chlamydia infection, as opposed to one of the control subjects (P = 0.00093). Eight of these 11 had clinical conjunctivitis, and two of the eight developed pneumonia. Growth retardation and developmental abnormalities were not detected in either group. It is concluded that maternal carriage of C. trachomatis is associated with a high incidence of clinical illness in the offspring.", "contents": "Chlamydial infection of mothers and their infants. In 340 women, cultured prospectively during their pregnancies, the rate of infection with Chlamydia trachomatis was 8.8%. The women with positive cultures tended to be younger and more often single and black than their counterparts with negative cultures. There were no statistically significant clinical differences between the two groups. Eighteen children born to Chlamydia culture-positive women and 16 born to negative women were followed for nine months to examine the potential effects of maternal infection on infant growth, development, and illness. Eleven of 18 study patients had culture or tear antibody evidence of Chlamydia infection, as opposed to one of the control subjects (P = 0.00093). Eight of these 11 had clinical conjunctivitis, and two of the eight developed pneumonia. Growth retardation and developmental abnormalities were not detected in either group. It is concluded that maternal carriage of C. trachomatis is associated with a high incidence of clinical illness in the offspring."}
{"id": "PMID:480011", "title": "The use of plasma androstenedione in monitoring therapy of patients with congenital adrenal hyperplasia.", "content": "Therapy of congenital adrenal hyperplasia tranditionally is monitored by the amount of urinary 17-KS. However, 24-hour urine collections are difficult to obtain and are often unreliable. Measurement of the plasma concentrations of androgens, such as delta or T, would therefore be a more convenient way to determine the efficacy of treatment. Over a period of 2 to 24 months, 23 patients were periodically assessed by clinical examination, bone age, and determinations of plasma delta, plasma T, and 24-hour urinary 17-KS. Plasma T concentration correlated well with clinical control in females and in preadolescent males, but not in infant and pubertal males. By contrast, plasma delta concentration correlated well with clinical control in either sex, regardless of stage of puberty. The present study suggests that monitoring plasma delta concentration is useful in the long-term management of patients with CAH.", "contents": "The use of plasma androstenedione in monitoring therapy of patients with congenital adrenal hyperplasia. Therapy of congenital adrenal hyperplasia tranditionally is monitored by the amount of urinary 17-KS. However, 24-hour urine collections are difficult to obtain and are often unreliable. Measurement of the plasma concentrations of androgens, such as delta or T, would therefore be a more convenient way to determine the efficacy of treatment. Over a period of 2 to 24 months, 23 patients were periodically assessed by clinical examination, bone age, and determinations of plasma delta, plasma T, and 24-hour urinary 17-KS. Plasma T concentration correlated well with clinical control in females and in preadolescent males, but not in infant and pubertal males. By contrast, plasma delta concentration correlated well with clinical control in either sex, regardless of stage of puberty. The present study suggests that monitoring plasma delta concentration is useful in the long-term management of patients with CAH."}
{"id": "PMID:480012", "title": "Autoantibodies to Tamm-Horsfall protein associated with urinary tract infections in girls.", "content": "Girls with various forms of urinary tract infections and a reference material were analyzed for autoantibodies in serum to the Tamm-Horsfall glycoprotein. Such antibodies could be detected in all sera analyzed. In the control subjects cord blood contained very low IgA and IgM anti-TH, which increased significantly up to the age of 8 months. The IgG anti-TH levels in cord blood correlated with maternal levels. After the age of 2 months the IgG anti-TH followed the anti-TH levels of the other immunoglobulin classes. Among the infants aged 2 to 7 months with acute UTI, no anti-TH increases were found. In girls more than one year of age with acute nonobstructive UTI, IgG and IgA anti-TH levels were significantly higher in those with acute pyelonephritis and reflux, with or without parenchymal reduction, than in those with acute pyelonephritis and normal radiologic findings. The latter group had significantly higher levels of IgG and IgA but not IgM anti-TH than did those with acute cystitits. In contrast, girls with renal parenchymal reduction but no signs of infection at the time of testing had significantly depressed anti-TH levels compared to control values.", "contents": "Autoantibodies to Tamm-Horsfall protein associated with urinary tract infections in girls. Girls with various forms of urinary tract infections and a reference material were analyzed for autoantibodies in serum to the Tamm-Horsfall glycoprotein. Such antibodies could be detected in all sera analyzed. In the control subjects cord blood contained very low IgA and IgM anti-TH, which increased significantly up to the age of 8 months. The IgG anti-TH levels in cord blood correlated with maternal levels. After the age of 2 months the IgG anti-TH followed the anti-TH levels of the other immunoglobulin classes. Among the infants aged 2 to 7 months with acute UTI, no anti-TH increases were found. In girls more than one year of age with acute nonobstructive UTI, IgG and IgA anti-TH levels were significantly higher in those with acute pyelonephritis and reflux, with or without parenchymal reduction, than in those with acute pyelonephritis and normal radiologic findings. The latter group had significantly higher levels of IgG and IgA but not IgM anti-TH than did those with acute cystitits. In contrast, girls with renal parenchymal reduction but no signs of infection at the time of testing had significantly depressed anti-TH levels compared to control values."}
{"id": "PMID:480023", "title": "The neonatal blood count in health and disease. I. Reference values for neutrophilic cells.", "content": "Reference ranges for absolute total neutrophils/mm3, absolute immature neutrophils/mm3, and the fraction of immature to total neutrophils (I:T proportion) during the first 28 days of life are developed from 585 peripheral blood counts obtained from 304 normal neonates and 320 counts obtained from 130 neonates with perinatal complications demonstrated to have no statistically significant effect on neutrophil dynamics. Perinatal factors other than bacterial disease which significantly alter neutrophil dynamics include maternal hypertension, maternal fever prior to delivery, hemolytic disease, and periventricular hemorrhage. The predictive value of these reference ranges in identifying bacterial disease in the first week of age varies with the neutrophil factor evaluated and the clinical setting. Neutropenia in the presence of respiratory distress in the first 72 hours had an 84% likelihood of signifying bacterial disease, whereas neutropenia in the presence of asphyxia had a 68% likelihood of signifying bacterial disease. An abnormal I:T proportion had an accuracy of 82% and 61%, respectively, in the same clinical settings. Elevations of either immature or total neutrophils were less specific. Interpretation of abnormal neutrophil factors must include consideration of both infectious and noninfectious perinatal events.", "contents": "The neonatal blood count in health and disease. I. Reference values for neutrophilic cells. Reference ranges for absolute total neutrophils/mm3, absolute immature neutrophils/mm3, and the fraction of immature to total neutrophils (I:T proportion) during the first 28 days of life are developed from 585 peripheral blood counts obtained from 304 normal neonates and 320 counts obtained from 130 neonates with perinatal complications demonstrated to have no statistically significant effect on neutrophil dynamics. Perinatal factors other than bacterial disease which significantly alter neutrophil dynamics include maternal hypertension, maternal fever prior to delivery, hemolytic disease, and periventricular hemorrhage. The predictive value of these reference ranges in identifying bacterial disease in the first week of age varies with the neutrophil factor evaluated and the clinical setting. Neutropenia in the presence of respiratory distress in the first 72 hours had an 84% likelihood of signifying bacterial disease, whereas neutropenia in the presence of asphyxia had a 68% likelihood of signifying bacterial disease. An abnormal I:T proportion had an accuracy of 82% and 61%, respectively, in the same clinical settings. Elevations of either immature or total neutrophils were less specific. Interpretation of abnormal neutrophil factors must include consideration of both infectious and noninfectious perinatal events."}
{"id": "PMID:480024", "title": "Eosinophilia in premature infants: relationship to weight gain.", "content": "Serial eosinophil counts were determined in 38 hospitalized, appropriately grown premature infants whose gestational ages ranged from 27 to 35 weeks. Absolute eosinophilia (greater than 700/mm3) was documented in 76% (29/38). Eosinophilia was mild (700 to 999/mm3) in 9, moderate (1,000 to 2,999/mm3) in 17, and marked (greater than or equal to 3,000/mm3) in 3 patients. The average time of onset was day 19. Peak eosinophilia was usually seen within one week of onset and lasted an average of 16 days. A consistent relationship (r = 0.86) was found between the day of peak eosinophilia (mean = day 27) and the day on which birthweight was regained (mean = day 22). No association was apparent between the occurrence or degree of eosinophilia and gestational age, birth stress, presence of umbilical catheter, parenteral alimentation solution, and time of beginning or type or oral feeds. The data suggest that eosinophilia is strongly associated with the establishment of an anabolic state.", "contents": "Eosinophilia in premature infants: relationship to weight gain. Serial eosinophil counts were determined in 38 hospitalized, appropriately grown premature infants whose gestational ages ranged from 27 to 35 weeks. Absolute eosinophilia (greater than 700/mm3) was documented in 76% (29/38). Eosinophilia was mild (700 to 999/mm3) in 9, moderate (1,000 to 2,999/mm3) in 17, and marked (greater than or equal to 3,000/mm3) in 3 patients. The average time of onset was day 19. Peak eosinophilia was usually seen within one week of onset and lasted an average of 16 days. A consistent relationship (r = 0.86) was found between the day of peak eosinophilia (mean = day 27) and the day on which birthweight was regained (mean = day 22). No association was apparent between the occurrence or degree of eosinophilia and gestational age, birth stress, presence of umbilical catheter, parenteral alimentation solution, and time of beginning or type or oral feeds. The data suggest that eosinophilia is strongly associated with the establishment of an anabolic state."}
{"id": "PMID:480026", "title": "Hyperlipoproteinemia and multifocal neurologic dysfunction in systemic lupus erythematosus.", "content": "Two patients with systemic lupus erythematosus, including one with neuropathologic findings, had recurrent multifocal neurologic dysfunction and hyperlipoproteinemia. The lipoprotein disturbances were complex and variable over time. Deficient lipoprotein lipase was found in both patients and appeared to be related temporally to neurologic deterioration. One of these patients had neurologic disease and lipoprotein abnormalities 2 1/2 years before SLE could be documented serologically. These studies suggest that lipoprotein lipase deficiency may be a marker for the endothelial disorder causing cerebral vasculopathy in SLE.", "contents": "Hyperlipoproteinemia and multifocal neurologic dysfunction in systemic lupus erythematosus. Two patients with systemic lupus erythematosus, including one with neuropathologic findings, had recurrent multifocal neurologic dysfunction and hyperlipoproteinemia. The lipoprotein disturbances were complex and variable over time. Deficient lipoprotein lipase was found in both patients and appeared to be related temporally to neurologic deterioration. One of these patients had neurologic disease and lipoprotein abnormalities 2 1/2 years before SLE could be documented serologically. These studies suggest that lipoprotein lipase deficiency may be a marker for the endothelial disorder causing cerebral vasculopathy in SLE."}
{"id": "PMID:480027", "title": "Holt-Oram syndrome.", "content": "The autosomal dominant association of upper extremity skeletal defects with congenital heart disease is known as the Holt-Oram syndrome. We reviewed our experience with 39 affected patients of whom 15 were considered new mutations. Wide varieties of skeletal defects and congenital heart disease were observed, and the severity of skeletal involvement did not parallel that of cardiac disease. These patients demonstrate four previously unemphasized points: (1) There is a striking asymmetry of skeletal involvement, with the left side more severely affected. (2) Patients with skeletal defects alone can transmit both skeletal and cardiac defects to their children. (3) Hypoplastic peripheral vessels may be an associated abnormality and can result in difficulty with cardiac catheterization. (4) Electrocardiographic changes of terminal conduction delay in the right anterior chest leads were nor uniformly present in patients with otherwise typical secundum atrial septal defects.", "contents": "Holt-Oram syndrome. The autosomal dominant association of upper extremity skeletal defects with congenital heart disease is known as the Holt-Oram syndrome. We reviewed our experience with 39 affected patients of whom 15 were considered new mutations. Wide varieties of skeletal defects and congenital heart disease were observed, and the severity of skeletal involvement did not parallel that of cardiac disease. These patients demonstrate four previously unemphasized points: (1) There is a striking asymmetry of skeletal involvement, with the left side more severely affected. (2) Patients with skeletal defects alone can transmit both skeletal and cardiac defects to their children. (3) Hypoplastic peripheral vessels may be an associated abnormality and can result in difficulty with cardiac catheterization. (4) Electrocardiographic changes of terminal conduction delay in the right anterior chest leads were nor uniformly present in patients with otherwise typical secundum atrial septal defects."}
{"id": "PMID:480028", "title": "The amniotic band disruption complex: timing of amniotic rupture and variable spectra of consequent defects.", "content": "Seventy-nine patients with the amniotic band disruption complex, including 54 infants with multiple system involvement and 25 with affected limbs alone, were evaluated. No two cases of the disorder were exactly alike. Defects varied from simple digital band constrictions to major craniofacial and visceral defects; fetal death may also occur. Amniotic rupture appeared to cause injury through three basic mechanisms: (1) interruption of normal morphogenesis; (2) crowing of fetal parts; and (3) disruption of previously differentiated structure. Comparison of 35 cases in which the timing of amniotic rupture could be estimated suggests that early amniotic rupture results in multiply affected infants who are frequently aborted or stillborn, whereas later rupture results primarily in limb involvement. Our findings indicate that both the spectrum of the developmental pathology and the nature of fetal outcome are determined by the timing of amniotic rupture. Appreciation of the mechanism which explains the disparate appearances of infants with the amniotic band disruption complex will allow more acurate diagnosis and appropriate counseling with respect to the sporadic nature of the disorder.", "contents": "The amniotic band disruption complex: timing of amniotic rupture and variable spectra of consequent defects. Seventy-nine patients with the amniotic band disruption complex, including 54 infants with multiple system involvement and 25 with affected limbs alone, were evaluated. No two cases of the disorder were exactly alike. Defects varied from simple digital band constrictions to major craniofacial and visceral defects; fetal death may also occur. Amniotic rupture appeared to cause injury through three basic mechanisms: (1) interruption of normal morphogenesis; (2) crowing of fetal parts; and (3) disruption of previously differentiated structure. Comparison of 35 cases in which the timing of amniotic rupture could be estimated suggests that early amniotic rupture results in multiply affected infants who are frequently aborted or stillborn, whereas later rupture results primarily in limb involvement. Our findings indicate that both the spectrum of the developmental pathology and the nature of fetal outcome are determined by the timing of amniotic rupture. Appreciation of the mechanism which explains the disparate appearances of infants with the amniotic band disruption complex will allow more acurate diagnosis and appropriate counseling with respect to the sporadic nature of the disorder."}
{"id": "PMID:480037", "title": "Primary pulmonary hypoplasia in the neonate.", "content": "Infants with primary pulmonary hypoplasia with respiratory distress immediately after birth, but usually elude early diagnosis. They have no other abnormalities, but frequently develop signs and symptoms of the PFC syndrome. Roentgenographically, they show small, clear lungs and are prone to develop complicating pneumothoraces. The clinical, roentgenographic, and pathologic features of eight infants with primary pulmonary hypoplasia are presented.", "contents": "Primary pulmonary hypoplasia in the neonate. Infants with primary pulmonary hypoplasia with respiratory distress immediately after birth, but usually elude early diagnosis. They have no other abnormalities, but frequently develop signs and symptoms of the PFC syndrome. Roentgenographically, they show small, clear lungs and are prone to develop complicating pneumothoraces. The clinical, roentgenographic, and pathologic features of eight infants with primary pulmonary hypoplasia are presented."}
{"id": "PMID:480039", "title": "Indomethacin and renal function in premature infants with persistent patent ductus arteriosus.", "content": "Renal function during indomethacin treatment was studied in 12 premature infants with patent ductus arteriosus. Decreases in urinary flow rate, GFR, and CH2O by 56, 27, and 66%, respectively, occurred during Indo therapy. Urinary excretion rates of ions were also reduced: Na by 70%, Cl by 79%, K by 40%. These changes were accompanied by slight decreases in plasma sodium concentration and osmolality. Except for GFR and urinary Na and osmolality, all these functions returned to pretreatment values one to two weeks after stopping the drug.", "contents": "Indomethacin and renal function in premature infants with persistent patent ductus arteriosus. Renal function during indomethacin treatment was studied in 12 premature infants with patent ductus arteriosus. Decreases in urinary flow rate, GFR, and CH2O by 56, 27, and 66%, respectively, occurred during Indo therapy. Urinary excretion rates of ions were also reduced: Na by 70%, Cl by 79%, K by 40%. These changes were accompanied by slight decreases in plasma sodium concentration and osmolality. Except for GFR and urinary Na and osmolality, all these functions returned to pretreatment values one to two weeks after stopping the drug."}
{"id": "PMID:480040", "title": "Patent ductus arteriosus treated with ligation or indomethacin: a follow-up study.", "content": "The course and complications of fifty-two infants with patent ductus arteriosus requiring closure were assessed prospectively. Twenty-six infants with a PDA received indomethacin for pharmacologic closure of the PDA, and 26 underwent ligation. The current study analyzes and compares the longitudinal follow-up with respect to somatic growth, neurologic function, psychomotor and mental development, and renal, ophthalmologic, and audiologic function in 21 infants in each group who entered the follow-up. No selective morbidity was attributable to PDA closure with indomethacin when compared to surgically treated infants.", "contents": "Patent ductus arteriosus treated with ligation or indomethacin: a follow-up study. The course and complications of fifty-two infants with patent ductus arteriosus requiring closure were assessed prospectively. Twenty-six infants with a PDA received indomethacin for pharmacologic closure of the PDA, and 26 underwent ligation. The current study analyzes and compares the longitudinal follow-up with respect to somatic growth, neurologic function, psychomotor and mental development, and renal, ophthalmologic, and audiologic function in 21 infants in each group who entered the follow-up. No selective morbidity was attributable to PDA closure with indomethacin when compared to surgically treated infants."}
{"id": "PMID:480041", "title": "Refractory hypoxemia associated with neonatal pulmonary disease: the use and limitations of tolazoline.", "content": "Thirty-nine critically ill infants with pulmonary disease received tolazoline because of severe hypoxemia refractory to administration of 100% O2 and mechanical ventilation. Twenty-seven (69%) of the infants responded with an increase in PaO2 greater than or equal to 20 torr in the first umbilical arterial gas after completion of the initial ten-minute infusion (1 to 2 mg/kg) of the drug. A response was not correlated with survival. The overall survival was 46%, essentially unchanged from our previous report (44%). Infants with hyaline membrane disease had the poorest survival rate (33%). Complications associated with the use of tolazoline occurred in 82% of the infants. A hypotensive reaction, defined as a 25% decrease in mean arterial pressure from the pre-tolazoline level, occurred in 67% of the infants, and more commonly in the infants with RDS (87%). In 11 infants who did not respond to the initial dose of tolazoline, the dose was increased up to 10 mg/kg/hour; only one infant responded, and eight (73%) had a hypotensive reaction.", "contents": "Refractory hypoxemia associated with neonatal pulmonary disease: the use and limitations of tolazoline. Thirty-nine critically ill infants with pulmonary disease received tolazoline because of severe hypoxemia refractory to administration of 100% O2 and mechanical ventilation. Twenty-seven (69%) of the infants responded with an increase in PaO2 greater than or equal to 20 torr in the first umbilical arterial gas after completion of the initial ten-minute infusion (1 to 2 mg/kg) of the drug. A response was not correlated with survival. The overall survival was 46%, essentially unchanged from our previous report (44%). Infants with hyaline membrane disease had the poorest survival rate (33%). Complications associated with the use of tolazoline occurred in 82% of the infants. A hypotensive reaction, defined as a 25% decrease in mean arterial pressure from the pre-tolazoline level, occurred in 67% of the infants, and more commonly in the infants with RDS (87%). In 11 infants who did not respond to the initial dose of tolazoline, the dose was increased up to 10 mg/kg/hour; only one infant responded, and eight (73%) had a hypotensive reaction."}
{"id": "PMID:480042", "title": "Direct and indirect pulmonary vascular effects of tolazoline in the newborn lamb.", "content": "The pulmonary vascular effects of tolazoline were studied in unsedated newborn lambs during normal oxygenation and hypoxia. Direct and indirect pulmonary vascular responses were analyzed separately. During normal oxygenation, tolazoline (1 mg/kg) given into a branch pulmonary artery increased cardiac output while decreasing systemic and pulmonary resistances. Pulmonary flow distribution did not change, suggesting that the fall in pulmonary resistance was due to an indirect rather than a direct action of the drug. Tolazoline had similar effects on systemic and pulmonary resistances in the hypoxic lamb; however, there was a shift in blood flow toward the injected lung, indicating local pulmonary vasodilation induced by the drug. In either case, tolazoline did not alter the resistance ratio between the injected lung and the systemic circulation. We conclude that tolazoline is a direct pulmonary vasodilator in the hypoxic lamb, but does not appear to lower the pulmonary to systemic resistance ratio.", "contents": "Direct and indirect pulmonary vascular effects of tolazoline in the newborn lamb. The pulmonary vascular effects of tolazoline were studied in unsedated newborn lambs during normal oxygenation and hypoxia. Direct and indirect pulmonary vascular responses were analyzed separately. During normal oxygenation, tolazoline (1 mg/kg) given into a branch pulmonary artery increased cardiac output while decreasing systemic and pulmonary resistances. Pulmonary flow distribution did not change, suggesting that the fall in pulmonary resistance was due to an indirect rather than a direct action of the drug. Tolazoline had similar effects on systemic and pulmonary resistances in the hypoxic lamb; however, there was a shift in blood flow toward the injected lung, indicating local pulmonary vasodilation induced by the drug. In either case, tolazoline did not alter the resistance ratio between the injected lung and the systemic circulation. We conclude that tolazoline is a direct pulmonary vasodilator in the hypoxic lamb, but does not appear to lower the pulmonary to systemic resistance ratio."}
{"id": "PMID:480043", "title": "Low cerebral blood flow: a risk factor in the neonate.", "content": "Among 19 infants in whom cerebral blood flow had been determined a few hours after birth, four died during the first days or weeks after birth, all with massive intracranial hemorrhage. The other infants were examined at 9 to 12 1/2 months of age by means of clinical neurologic evaluation, developmental psychologic assessment (Cattell), EEG, and cranial computed tomography. Six of the ten infants who had had CBF of 20 ml/100 gm/minute or less had developed cerebral atrophy as demonstrated at autopsy or by CT scan, none with neonatal flows above 20 had done so. Only one in the low flow group had developed completely normally, whereas abnormal development was found in only a minority of the high flow group. No other neonatal observation had such a clear relationship to later development. It is concluded that CBF of 20 or less during the first hours of life is critical.", "contents": "Low cerebral blood flow: a risk factor in the neonate. Among 19 infants in whom cerebral blood flow had been determined a few hours after birth, four died during the first days or weeks after birth, all with massive intracranial hemorrhage. The other infants were examined at 9 to 12 1/2 months of age by means of clinical neurologic evaluation, developmental psychologic assessment (Cattell), EEG, and cranial computed tomography. Six of the ten infants who had had CBF of 20 ml/100 gm/minute or less had developed cerebral atrophy as demonstrated at autopsy or by CT scan, none with neonatal flows above 20 had done so. Only one in the low flow group had developed completely normally, whereas abnormal development was found in only a minority of the high flow group. No other neonatal observation had such a clear relationship to later development. It is concluded that CBF of 20 or less during the first hours of life is critical."}
{"id": "PMID:480047", "title": "Aspirin dosage for infants and children.", "content": "The labeled dosage schedule that has long been on pediatric aspirin preparations is at variance with the recommendation in authoritative medical references, studies demonstrating antipyretic effectiveness in children, and the prescribing habits of pediatricians as revealed by a poll conducted by the authors. Aspirin pharmacokinetics are influenced by a number of physiologic factors, as well as by dosage, and complicate the problem of assuring safe and effective pediatric use. Basic pharmacokinetic considerations indicate that the increase in size of individual doses needed to assure therapeutic salicyate blood levels, thereby removing the temptation of parents to administer the drug too often, is made permissible (i.e., safe while effective) by expanding the interdose interval to four hours instead of three. A revised pediatric aspirin disage schedule is presented that better meets the practices of the pediatric community and the needs of consumers.", "contents": "Aspirin dosage for infants and children. The labeled dosage schedule that has long been on pediatric aspirin preparations is at variance with the recommendation in authoritative medical references, studies demonstrating antipyretic effectiveness in children, and the prescribing habits of pediatricians as revealed by a poll conducted by the authors. Aspirin pharmacokinetics are influenced by a number of physiologic factors, as well as by dosage, and complicate the problem of assuring safe and effective pediatric use. Basic pharmacokinetic considerations indicate that the increase in size of individual doses needed to assure therapeutic salicyate blood levels, thereby removing the temptation of parents to administer the drug too often, is made permissible (i.e., safe while effective) by expanding the interdose interval to four hours instead of three. A revised pediatric aspirin disage schedule is presented that better meets the practices of the pediatric community and the needs of consumers."}
{"id": "PMID:480048", "title": "Comparative effects of naproxen and aspirin on fever in children.", "content": "The antipyretic effect of single doses of naproxen, 2.5 mg/kg or 7.5 mg/kg, was compared to that of aspirin, 15 mg/kg, and of a placebo in a double-blind study. Febrile children, hospitalized overnight, were randomly assigned to one of the treatment or placebo groups, and their temperatures were recorded hourly. Naproxen, 7.5 mg/kg, was at least as effective as aspirin in onset of action and reduction of fever, and had a longer duration of action. Each of the three treatments had a significant effect as compared to that of the placebo. There was no difference in the total incidence of side effects among the four groups, but mild gastrointestinal complaints were more common among the patients in the high-dose naproxen group, whereas CNS complaints were more prevalent among the patients in the placebo group.", "contents": "Comparative effects of naproxen and aspirin on fever in children. The antipyretic effect of single doses of naproxen, 2.5 mg/kg or 7.5 mg/kg, was compared to that of aspirin, 15 mg/kg, and of a placebo in a double-blind study. Febrile children, hospitalized overnight, were randomly assigned to one of the treatment or placebo groups, and their temperatures were recorded hourly. Naproxen, 7.5 mg/kg, was at least as effective as aspirin in onset of action and reduction of fever, and had a longer duration of action. Each of the three treatments had a significant effect as compared to that of the placebo. There was no difference in the total incidence of side effects among the four groups, but mild gastrointestinal complaints were more common among the patients in the high-dose naproxen group, whereas CNS complaints were more prevalent among the patients in the placebo group."}
{"id": "PMID:480050", "title": "Hydrocephalus in tuberculous meningitis in children: treatment with acetazolamide and repeated lumbar puncture.", "content": "Increased intracranial pressure was found in 36 of 49 children with tuberculous meningitis. Four patients who presented with acute onset died early. Eight patients with obstructive hydrocephalus were treated surgically. In the 24 patients who had communicating hydrocephalus and were treated with acetazolamide and repeated lumbar punctures, treatment was successful in all but two, in whom shunt surgery was needed.", "contents": "Hydrocephalus in tuberculous meningitis in children: treatment with acetazolamide and repeated lumbar puncture. Increased intracranial pressure was found in 36 of 49 children with tuberculous meningitis. Four patients who presented with acute onset died early. Eight patients with obstructive hydrocephalus were treated surgically. In the 24 patients who had communicating hydrocephalus and were treated with acetazolamide and repeated lumbar punctures, treatment was successful in all but two, in whom shunt surgery was needed."}
{"id": "PMID:480062", "title": "Argentophilic papillae of Schistosomatium douthitti cercariae (Trematoda: Schistosomatidae).", "content": "Argentophilic papillae of cercariae of Schistosomatium douthitti were studied by light microscopy. The basic number of body papillae was 56, excluding 20 at the anterior tip. The average number of tail papillae was 18. Average locations and spatial ranges of body papillae indicate a rather constant pattern. Tail papillae showed greater variability in location. Twenty papillae (10 on each side) at the anterior tip of cercariae were associated with gland duct openings. Two types of papillae were recognized with light microscopy--uniciliated bulbs and pits. The papilla pattern of S. douthitti differs from those of other species of schistosomes derived to date, and its constancy supports the usefulness of papillae in systematic studies.", "contents": "Argentophilic papillae of Schistosomatium douthitti cercariae (Trematoda: Schistosomatidae). Argentophilic papillae of cercariae of Schistosomatium douthitti were studied by light microscopy. The basic number of body papillae was 56, excluding 20 at the anterior tip. The average number of tail papillae was 18. Average locations and spatial ranges of body papillae indicate a rather constant pattern. Tail papillae showed greater variability in location. Twenty papillae (10 on each side) at the anterior tip of cercariae were associated with gland duct openings. Two types of papillae were recognized with light microscopy--uniciliated bulbs and pits. The papilla pattern of S. douthitti differs from those of other species of schistosomes derived to date, and its constancy supports the usefulness of papillae in systematic studies."}
{"id": "PMID:480063", "title": "Transintegumental uptake of metabolic substrates in male and female Schistosoma mansoni.", "content": "A new method for measuring transintegumental uptake in living schistosomes in vitro has been applied to the study of individual males and females. Uptake of a 14-C labeled test metabolite was compared to that of tritiated water (a highly diffusible reference substance). Use of the short half-life (T 1/2 = 100 min) isotope 113m-Indium, bound to EDTA (ethylene diamine tetra-acetic acid, a nondiffusible reference substance) permitted quantification of the relative amount of 14-C test substance passively adhering to the schistosoma surface. Substraction of this amount provided an estimate of net uptake. D-glucose uptake, as measured by this method, increased with time, approaching equilibrium by two min; a positive correlation between temperature and glucose uptake was also observed. Nondialyzable components in rat, human, horse and fetal calf sera did not enhance glucose uptake. In both male and female schistosomes, minimal uptakes were seen for the nonmetabolizable sugar alcohol mannitol (MW = 182). L-glucose uptake was similarly low, but high uptakes were observed in both sexes for D-glucose. In addition to confirming the stereospecificity of hexose uptake, these studies suggested our technique provides a sensitive method for measurement of both high and low uptake compounds. The uptakes of D-glucose and the L-amino acids--arginine, ornithine, lysine, histidine, phenylalanine and serine--were comparatively higher in female than male schistosomes. Slight elevations in uptake by females were observed for threonine, valine and glycine, but aspartate uptake was slightly higher in males. No dramatic male-female differences were immediately apparent for the uptakes of proline, leucine, isoleucine, tyrosine and glutamate. Schistosomal uptake of L-amino acids that are essential for vertebrates was generally higher than uptake of the nonessential amino acids.", "contents": "Transintegumental uptake of metabolic substrates in male and female Schistosoma mansoni. A new method for measuring transintegumental uptake in living schistosomes in vitro has been applied to the study of individual males and females. Uptake of a 14-C labeled test metabolite was compared to that of tritiated water (a highly diffusible reference substance). Use of the short half-life (T 1/2 = 100 min) isotope 113m-Indium, bound to EDTA (ethylene diamine tetra-acetic acid, a nondiffusible reference substance) permitted quantification of the relative amount of 14-C test substance passively adhering to the schistosoma surface. Substraction of this amount provided an estimate of net uptake. D-glucose uptake, as measured by this method, increased with time, approaching equilibrium by two min; a positive correlation between temperature and glucose uptake was also observed. Nondialyzable components in rat, human, horse and fetal calf sera did not enhance glucose uptake. In both male and female schistosomes, minimal uptakes were seen for the nonmetabolizable sugar alcohol mannitol (MW = 182). L-glucose uptake was similarly low, but high uptakes were observed in both sexes for D-glucose. In addition to confirming the stereospecificity of hexose uptake, these studies suggested our technique provides a sensitive method for measurement of both high and low uptake compounds. The uptakes of D-glucose and the L-amino acids--arginine, ornithine, lysine, histidine, phenylalanine and serine--were comparatively higher in female than male schistosomes. Slight elevations in uptake by females were observed for threonine, valine and glycine, but aspartate uptake was slightly higher in males. No dramatic male-female differences were immediately apparent for the uptakes of proline, leucine, isoleucine, tyrosine and glutamate. Schistosomal uptake of L-amino acids that are essential for vertebrates was generally higher than uptake of the nonessential amino acids."}
{"id": "PMID:480064", "title": "Comparison of carbon and nitrogen content of infected and uninfected snails, Succinea ovalis, and the trematode Leucochloridium variae.", "content": "In June, 6.7% of adult Succinea ovalis collected near Urbana, Ohio, were infected with the trematode, Leucochloridium variae. The effects of parasitism were assessed as total organic carbon (equivalent to calorific values) and as total nitrogen. The parasite represents 23.8% of total (parasite + snail tissue) dry tissue weight, 21.4% of total carbon and 19.8% of total nitrogen of infected snails. The higher C : N ratio for parasite tissue indicates a higher proportion of nonproteinaceous compounds (e.g., fats and/or carbohydrates) as compared to host tissue. There is less snail tissue in parasited S. ovalis. The C : N ratios for parasitized and nonparasitized snail tissue suggest identical percentage compositions of proteins, fats, and carbohydrates.", "contents": "Comparison of carbon and nitrogen content of infected and uninfected snails, Succinea ovalis, and the trematode Leucochloridium variae. In June, 6.7% of adult Succinea ovalis collected near Urbana, Ohio, were infected with the trematode, Leucochloridium variae. The effects of parasitism were assessed as total organic carbon (equivalent to calorific values) and as total nitrogen. The parasite represents 23.8% of total (parasite + snail tissue) dry tissue weight, 21.4% of total carbon and 19.8% of total nitrogen of infected snails. The higher C : N ratio for parasite tissue indicates a higher proportion of nonproteinaceous compounds (e.g., fats and/or carbohydrates) as compared to host tissue. There is less snail tissue in parasited S. ovalis. The C : N ratios for parasitized and nonparasitized snail tissue suggest identical percentage compositions of proteins, fats, and carbohydrates."}
{"id": "PMID:480065", "title": "A new species of Breinlia (Breinlia) (nematoda: Filarioidea) from the South Indian flying squirrel Petaurista philippensis (Elliot).", "content": "Breinlia (Breinlia) petauristi sp. n. from the abdominal cavity of the flying squirrel, Petaurista philippensis (Elliot), from Kerala, India, is described. Of the 15 recognized species of the subgenus, B. petauristi most closely resembles B. trichosuri in the body size, length of spicules, and number of caudal papillae. The detailed structure of the spicules is, however, markedly different in the 2 worms. This is the third species of Breinlia to be reported from a host other than an Australian marsupial.", "contents": "A new species of Breinlia (Breinlia) (nematoda: Filarioidea) from the South Indian flying squirrel Petaurista philippensis (Elliot). Breinlia (Breinlia) petauristi sp. n. from the abdominal cavity of the flying squirrel, Petaurista philippensis (Elliot), from Kerala, India, is described. Of the 15 recognized species of the subgenus, B. petauristi most closely resembles B. trichosuri in the body size, length of spicules, and number of caudal papillae. The detailed structure of the spicules is, however, markedly different in the 2 worms. This is the third species of Breinlia to be reported from a host other than an Australian marsupial."}
{"id": "PMID:480066", "title": "Studies on the Onchocerca (nematoda: Filarioidea) found in cattle in the United States. I. Systematics of O. gutturosa and O. lienalis with a description of O. stilesi sp. n.", "content": "Onchocerca gutturosa and O. lienalis are redescribed and comparative morphological descriptions provided. In addition, a third species of Onchocerca is described from cattle in the United States. This third species, Onchocerca stilesi sp. n., localizes in the connective tissues around the stifle joint, and can be distinguished morphologically from O. gutterosa and O. lienalis. Onchocerca gutturosa and O. lienalis apparently have widespread distribution in the eastern United States; however O. stilesi has been reported only from Minnesota.", "contents": "Studies on the Onchocerca (nematoda: Filarioidea) found in cattle in the United States. I. Systematics of O. gutturosa and O. lienalis with a description of O. stilesi sp. n. Onchocerca gutturosa and O. lienalis are redescribed and comparative morphological descriptions provided. In addition, a third species of Onchocerca is described from cattle in the United States. This third species, Onchocerca stilesi sp. n., localizes in the connective tissues around the stifle joint, and can be distinguished morphologically from O. gutterosa and O. lienalis. Onchocerca gutturosa and O. lienalis apparently have widespread distribution in the eastern United States; however O. stilesi has been reported only from Minnesota."}
{"id": "PMID:480067", "title": "Redescription of Camallanus ancylodirus Ward and Magath 1916 (nematoda: Camallanidae) from freshwater fishes of North America.", "content": "Camallanus ancylodirus Ward and Magath 1916 is redescribed, based on examination of syntypes. This species differs from C. oxycephalus Ward and Magath 1916, the only other camallanid described from freshwater fishes of North America, in the size of male worms, number and arrangement of caudal papillae in males, shape of the male caudal end, position of the vulva, and length and shape of the female tail. The host range of C. ancylodirus is not clear from literature reports because the species may have been confused with C. oxycephalus.", "contents": "Redescription of Camallanus ancylodirus Ward and Magath 1916 (nematoda: Camallanidae) from freshwater fishes of North America. Camallanus ancylodirus Ward and Magath 1916 is redescribed, based on examination of syntypes. This species differs from C. oxycephalus Ward and Magath 1916, the only other camallanid described from freshwater fishes of North America, in the size of male worms, number and arrangement of caudal papillae in males, shape of the male caudal end, position of the vulva, and length and shape of the female tail. The host range of C. ancylodirus is not clear from literature reports because the species may have been confused with C. oxycephalus."}
{"id": "PMID:480068", "title": "Eimeria azul sp. n. (protozoa: Eimeriidae) from the eastern cottontail, Sylvilagus floridanus, in Pennsylvania.", "content": "Eimeria azul sp. n. is described from the cottontail rabbit, Sylvilagus floridanus, in central Pennsylvania. The oval oocysts are 19.5--27.0 micrometer by 15.0--19.0 (mean - 22.9 X 16.7 micrometer). The fusiform sporocysts are 7.8--14.0 micrometer by 3.3--6.5 micrometer (mean = 11.8 X 5.8 micrometer). A Stieda Body is present. There is no micropyle, oocyst residuum or polar granule. The sporocyst has a residuum which is variable in appearance. The oocysts are characterized by a blue tint when viewed with an apochromatic objective lens.", "contents": "Eimeria azul sp. n. (protozoa: Eimeriidae) from the eastern cottontail, Sylvilagus floridanus, in Pennsylvania. Eimeria azul sp. n. is described from the cottontail rabbit, Sylvilagus floridanus, in central Pennsylvania. The oval oocysts are 19.5--27.0 micrometer by 15.0--19.0 (mean - 22.9 X 16.7 micrometer). The fusiform sporocysts are 7.8--14.0 micrometer by 3.3--6.5 micrometer (mean = 11.8 X 5.8 micrometer). A Stieda Body is present. There is no micropyle, oocyst residuum or polar granule. The sporocyst has a residuum which is variable in appearance. The oocysts are characterized by a blue tint when viewed with an apochromatic objective lens."}
{"id": "PMID:480069", "title": "The Ornithodoros (Alectorobius) capensis group (Acarina: Ixodoidea: Argasidae) of the palearctic and oriental regions. O. (A.) coniceps identity, bird and mammal hosts, virus infections, and distribution in Europe, Africa, and Asia.", "content": "Ornithodoros (Alectorobius) coniceps (Canestrini 1890), which was briefly described from adult specimens from St. Mark's Cathedral, Venice, Italy, has been a confusing taxon. We redescribe and illustrate the adult and immature stages from St. Mark's Cathedral (topotypes) and elsewhere, list criteria to distinguish this species from others in the O. (A.) capensis complex, and provide data for 36 collections, chiefly from nest sites of wild and domestic pigeons in humid, rocky situations, and from a nest of the pallid swift, in Italy, France, Egypt, Kenya, Israel, Jordan, Afghanistan, Ukrainia, and Turkmenia. Other collections of small-sized adults and nymphs, lacking larvae, from pigeon, swallow, and swift nest in Nepal, India, and Sri Lanka, are tentatively assigned to this taxon. This is the only member of the O. (A.) capensis group not associated with marine or wading birds. Humans bitten by O. (A.) coniceps in buildings or caves may suffer from pain, edema, chills, and fever. BAku virus (Reoviridae) has been isolated from this tick in Uzbekistan, but most reports of other viruses and infectious agents are clouded by probable misidentification of O. (A.) maritimus as O. (A. coniceps. Published data on the life cycle and dynamics are reviewed briefly. Previously, this tick has not been reported from the Ethiopian Faunal Region (Kenya).", "contents": "The Ornithodoros (Alectorobius) capensis group (Acarina: Ixodoidea: Argasidae) of the palearctic and oriental regions. O. (A.) coniceps identity, bird and mammal hosts, virus infections, and distribution in Europe, Africa, and Asia. Ornithodoros (Alectorobius) coniceps (Canestrini 1890), which was briefly described from adult specimens from St. Mark's Cathedral, Venice, Italy, has been a confusing taxon. We redescribe and illustrate the adult and immature stages from St. Mark's Cathedral (topotypes) and elsewhere, list criteria to distinguish this species from others in the O. (A.) capensis complex, and provide data for 36 collections, chiefly from nest sites of wild and domestic pigeons in humid, rocky situations, and from a nest of the pallid swift, in Italy, France, Egypt, Kenya, Israel, Jordan, Afghanistan, Ukrainia, and Turkmenia. Other collections of small-sized adults and nymphs, lacking larvae, from pigeon, swallow, and swift nest in Nepal, India, and Sri Lanka, are tentatively assigned to this taxon. This is the only member of the O. (A.) capensis group not associated with marine or wading birds. Humans bitten by O. (A.) coniceps in buildings or caves may suffer from pain, edema, chills, and fever. BAku virus (Reoviridae) has been isolated from this tick in Uzbekistan, but most reports of other viruses and infectious agents are clouded by probable misidentification of O. (A.) maritimus as O. (A. coniceps. Published data on the life cycle and dynamics are reviewed briefly. Previously, this tick has not been reported from the Ethiopian Faunal Region (Kenya)."}
{"id": "PMID:480071", "title": "A malarial parasite of Australian skinks, Plasmodium mackerrasae sp. n.", "content": "Plasmodium mackerrasae sp. n. parasitizes the Australian lizards Egernia cunninghami and E. striolata (Sauria: Scincidae). Described from an experimental host, E. whitei, it produces mature schizonts containing 6--12 nuclei arranged peripherally as a rosette, and round to oval gametocytes which are equal to or slightly smaller than host cell nuclei. Both schizonts and gametocytes parasitize all cells in the erythrocyte series. Presence of pigment in both asexual and sexual stages is correlated with maturity of the host cell. Asexual forms contain a single large vacuole, whereas mature gametocytes may show 1--4 vacuoles. Plasmodium mackerrasae resembles most closely P. sasai of Japan and P. tropiduri of tropical America. It differs from P. sasai by lacking fan-shaped schizonts and by having less heavily pigmented gametocytes, and from P. tropiduri by less variability in shape and greater vacuolation of the gametocytes. Host and geographic differences further support its distinction.", "contents": "A malarial parasite of Australian skinks, Plasmodium mackerrasae sp. n. Plasmodium mackerrasae sp. n. parasitizes the Australian lizards Egernia cunninghami and E. striolata (Sauria: Scincidae). Described from an experimental host, E. whitei, it produces mature schizonts containing 6--12 nuclei arranged peripherally as a rosette, and round to oval gametocytes which are equal to or slightly smaller than host cell nuclei. Both schizonts and gametocytes parasitize all cells in the erythrocyte series. Presence of pigment in both asexual and sexual stages is correlated with maturity of the host cell. Asexual forms contain a single large vacuole, whereas mature gametocytes may show 1--4 vacuoles. Plasmodium mackerrasae resembles most closely P. sasai of Japan and P. tropiduri of tropical America. It differs from P. sasai by lacking fan-shaped schizonts and by having less heavily pigmented gametocytes, and from P. tropiduri by less variability in shape and greater vacuolation of the gametocytes. Host and geographic differences further support its distinction."}
{"id": "PMID:480072", "title": "Antibody to fibrinogen/fibrin products (Anti-F) in malaria, babesiosis, and trypanosomiasis of rodents.", "content": "Antibody to fibrinogen/fibrin related products (Anti-F) was stimulated during the course of Plasmodium chabaudi, Babesia rodhaini, and Trypanosoma lewisi infections in rats. Titers of this autoantibody remained elevated in serum from rats that had recovered from each of the infections. Column chromatographic studies indicated that Anti-F was a 19S globulin, possibly IgM. During acute infections high titers of Anti-F were associated with elevated titers of cold-active hemagglutinin (CAH) and immunoconglutinin (IK) and all were associated with anemia and elevated parasitemia. Titers of Anti-F and IK, but not CAH, remained elevated in serum of recovered rats. The presence of Anti-F indicated that the coagulation system had been activated during each infection to release fibrinogen/fibrin-related products (FRP) to serve as antigen(s) for Anti-F. Since IK is antibody to the third component of fixed complement, it could be assumed that complement fixing antigen-antibody complexes were also present during the acute stage of each infection. The possibility that complexes of FRP and Anti-F could have contributed to anemia in each infection is discussed.", "contents": "Antibody to fibrinogen/fibrin products (Anti-F) in malaria, babesiosis, and trypanosomiasis of rodents. Antibody to fibrinogen/fibrin related products (Anti-F) was stimulated during the course of Plasmodium chabaudi, Babesia rodhaini, and Trypanosoma lewisi infections in rats. Titers of this autoantibody remained elevated in serum from rats that had recovered from each of the infections. Column chromatographic studies indicated that Anti-F was a 19S globulin, possibly IgM. During acute infections high titers of Anti-F were associated with elevated titers of cold-active hemagglutinin (CAH) and immunoconglutinin (IK) and all were associated with anemia and elevated parasitemia. Titers of Anti-F and IK, but not CAH, remained elevated in serum of recovered rats. The presence of Anti-F indicated that the coagulation system had been activated during each infection to release fibrinogen/fibrin-related products (FRP) to serve as antigen(s) for Anti-F. Since IK is antibody to the third component of fixed complement, it could be assumed that complement fixing antigen-antibody complexes were also present during the acute stage of each infection. The possibility that complexes of FRP and Anti-F could have contributed to anemia in each infection is discussed."}
{"id": "PMID:480073", "title": "Susceptibility of five strains of mice to Babesia microti of human origin.", "content": "One outbred (CF1) and four inbred (BALB/c, C57, CBA and C3H) strains of mice were tested for susceptibility to Babesia microti of human origin. Of these, intact C3H mice developed higher parasitemia than all other intact mice, while BALB/c mice developed the highest parasitemia among splenectomized mice. Susceptibility was not related to H-2 haplotype in any obvious way. Because C3H and BALB/c mice developed relatively high initial peak parasitemias, the parasite was serially passaged in both of these mouse strains in an attempt to increase parasite virulence. After 30 passages in BALB/c and 49 passages in C3H mice over a period of 12 months, maximum parasitemias were 50 times higher than those observed initially. After the peak parasitemias of these two mouse-adapted parasites had stabilized, the relationship between onset and level of maximum parasitemia and number of parasites inoculated was determined. With both C3H- and BALB/c-adapted parasites, as inoculum size increased, the time required to reach maximum parasitemia decreased and the level of maximum parasitemia increased. Studies involving infection of either mouse strain with parasites adapted to the heterologous mouse strain indicated that C3H mice were more susceptible than BALB/c mice to homologous or heterologous parasites. These data suggest that the virulence of B. microti to the mouse can be increased by prolonged passage in this host. Once adaptation to this host species has occurred, virulence appears to be more dependent on the innate susceptibility of the mouse strain than on adaptation of the parasites to a particular strain of mouse.", "contents": "Susceptibility of five strains of mice to Babesia microti of human origin. One outbred (CF1) and four inbred (BALB/c, C57, CBA and C3H) strains of mice were tested for susceptibility to Babesia microti of human origin. Of these, intact C3H mice developed higher parasitemia than all other intact mice, while BALB/c mice developed the highest parasitemia among splenectomized mice. Susceptibility was not related to H-2 haplotype in any obvious way. Because C3H and BALB/c mice developed relatively high initial peak parasitemias, the parasite was serially passaged in both of these mouse strains in an attempt to increase parasite virulence. After 30 passages in BALB/c and 49 passages in C3H mice over a period of 12 months, maximum parasitemias were 50 times higher than those observed initially. After the peak parasitemias of these two mouse-adapted parasites had stabilized, the relationship between onset and level of maximum parasitemia and number of parasites inoculated was determined. With both C3H- and BALB/c-adapted parasites, as inoculum size increased, the time required to reach maximum parasitemia decreased and the level of maximum parasitemia increased. Studies involving infection of either mouse strain with parasites adapted to the heterologous mouse strain indicated that C3H mice were more susceptible than BALB/c mice to homologous or heterologous parasites. These data suggest that the virulence of B. microti to the mouse can be increased by prolonged passage in this host. Once adaptation to this host species has occurred, virulence appears to be more dependent on the innate susceptibility of the mouse strain than on adaptation of the parasites to a particular strain of mouse."}
{"id": "PMID:480074", "title": "Trichinella spiralis: an intracellular parasite in the intestinal phase.", "content": "Trichinella spiralis has been examined by electron microscopy after fixation in situ in the intestine of mice. The worms lie within the cytoplasm of cells of the intestinal mucosa and may occupy both absorptive and goblet cells. They cause little damage to host cells. A few worms have been seen protruding from tissue by SEM techniques. These unusual observations suggest that the nematodes may be capable of exit and reentry into the epithelium.", "contents": "Trichinella spiralis: an intracellular parasite in the intestinal phase. Trichinella spiralis has been examined by electron microscopy after fixation in situ in the intestine of mice. The worms lie within the cytoplasm of cells of the intestinal mucosa and may occupy both absorptive and goblet cells. They cause little damage to host cells. A few worms have been seen protruding from tissue by SEM techniques. These unusual observations suggest that the nematodes may be capable of exit and reentry into the epithelium."}
{"id": "PMID:480075", "title": "The effects of miracidal aging and dilution of snail-conditioned water on responses of miracidia of Megalodiscus temperatus.", "content": "Miracidia of Megalodiscus temperatus from newly hatched until 10 hr old were tested for their ability to react to Helisoma trivolvis snail-conditioned water (SCW) by contact with return (CR) to agar blocks and by percentage of miracidia reacting to a point inoculation of SCW as determined by a photographic time exposure method. CR to agar blocks containing 1:50 SCW was greatest during the first 6 hr after hatching but declined  thereafter. The reaction during the first hour to a point inoculation was lower than during the 2nd and 3rd hr. Results were variable from 4 to 10 hr after hatching with the lowest response recorded from 9 to 10 hr. Miracidial responses to dilutions of SCW were assessed by the same two methods. CR to agar blocks containing decreasing concentrations of SCW declined until at a dilution of 1:500 CR was only slightly above the controls. On the other hand, miracidial reactions to point inoculations of SCW as determined by the photographic method were still apparent at a dilution of 1:25,000, when 12% of the miracidia tested reacted. Thus, the photographic time exposure method gives a sensitive means for detecting altered miracidial behavior to various intrinsic and extrinsic factors.", "contents": "The effects of miracidal aging and dilution of snail-conditioned water on responses of miracidia of Megalodiscus temperatus. Miracidia of Megalodiscus temperatus from newly hatched until 10 hr old were tested for their ability to react to Helisoma trivolvis snail-conditioned water (SCW) by contact with return (CR) to agar blocks and by percentage of miracidia reacting to a point inoculation of SCW as determined by a photographic time exposure method. CR to agar blocks containing 1:50 SCW was greatest during the first 6 hr after hatching but declined  thereafter. The reaction during the first hour to a point inoculation was lower than during the 2nd and 3rd hr. Results were variable from 4 to 10 hr after hatching with the lowest response recorded from 9 to 10 hr. Miracidial responses to dilutions of SCW were assessed by the same two methods. CR to agar blocks containing decreasing concentrations of SCW declined until at a dilution of 1:500 CR was only slightly above the controls. On the other hand, miracidial reactions to point inoculations of SCW as determined by the photographic method were still apparent at a dilution of 1:25,000, when 12% of the miracidia tested reacted. Thus, the photographic time exposure method gives a sensitive means for detecting altered miracidial behavior to various intrinsic and extrinsic factors."}
{"id": "PMID:480077", "title": "Coccidia of wombats: correction of host-parasite relationships. Eimeria wombati (Gilruth and Bull, 1912) Comb. Nov. and Eimeria ursini Supperer, 1957 from the hairy-nosed wombat and Eimeria arundeli sp. n. from the common wombat.", "content": "Coccidial oocysts morphologically consistent with Eimeria ursini Supperer 1957, and E. tasmaniae Supperer 1957 were recovered from the feces of wild and captive hairy-nosed wombats (Lasiorhinus latifrons) in Australia. Eimeria arundeli so. n. was recovered from the feces of wild and captive common wombats (Vombatus ursinus). Eimeria arundeli oocysts are ellipsoidal to slightly ovoid 60.2--67.2 (63.7) X 40.6--47.6 (43.4); micropyle 3 in diameter usually visible; with oocyst wall granular, dark brown and occasionally opaque, 4--7 thick; inner oocyst wall clear, about 1.5 thick; small oocyst residuum present, four sporocysts ovoid 22.4--29.4 (25.8) X 12.6--15.4 (14.1) with protuberant Stieda body; opposite end of sporocyst also often slighly pointed; large granular sporocyst residuum obscuring sporozoites. Gametocytes of E. arundeli sp. n. and of an organism which is consistent with E. tasmaniae, are described developing in the lamina propria of villi in the small intestine. The stages in the hairy-nosed wombat are those described as Ileocystis wombati Gilruth and Bull 1912. It is suggested that the identification of the host of Supperer's E. ursini and E. tasmaniae as V. ursinus was in error and that the allopatric L. latifrons is the natural host. Eimeria tasmaniae Supperer 1957 is suppressed and E. wombati (Gilruth and Bull, 1912) comb. nov. is proposed and redescribed. No schizonts were identified among the endogenous stages, consistent with observations in the literature on other coccidia with similar gametocyte and oocyst structure.", "contents": "Coccidia of wombats: correction of host-parasite relationships. Eimeria wombati (Gilruth and Bull, 1912) Comb. Nov. and Eimeria ursini Supperer, 1957 from the hairy-nosed wombat and Eimeria arundeli sp. n. from the common wombat. Coccidial oocysts morphologically consistent with Eimeria ursini Supperer 1957, and E. tasmaniae Supperer 1957 were recovered from the feces of wild and captive hairy-nosed wombats (Lasiorhinus latifrons) in Australia. Eimeria arundeli so. n. was recovered from the feces of wild and captive common wombats (Vombatus ursinus). Eimeria arundeli oocysts are ellipsoidal to slightly ovoid 60.2--67.2 (63.7) X 40.6--47.6 (43.4); micropyle 3 in diameter usually visible; with oocyst wall granular, dark brown and occasionally opaque, 4--7 thick; inner oocyst wall clear, about 1.5 thick; small oocyst residuum present, four sporocysts ovoid 22.4--29.4 (25.8) X 12.6--15.4 (14.1) with protuberant Stieda body; opposite end of sporocyst also often slighly pointed; large granular sporocyst residuum obscuring sporozoites. Gametocytes of E. arundeli sp. n. and of an organism which is consistent with E. tasmaniae, are described developing in the lamina propria of villi in the small intestine. The stages in the hairy-nosed wombat are those described as Ileocystis wombati Gilruth and Bull 1912. It is suggested that the identification of the host of Supperer's E. ursini and E. tasmaniae as V. ursinus was in error and that the allopatric L. latifrons is the natural host. Eimeria tasmaniae Supperer 1957 is suppressed and E. wombati (Gilruth and Bull, 1912) comb. nov. is proposed and redescribed. No schizonts were identified among the endogenous stages, consistent with observations in the literature on other coccidia with similar gametocyte and oocyst structure."}
{"id": "PMID:480078", "title": "Diclidophora embiotocae sp. n. (Monogenea, Diclidophoridae), a parasite of embiotocid fishes of Oregon.", "content": "Diclidophora embiotocae sp. n. is described from adult specimens obtained from the gills of three species of embiotocid fishes from the central coast of Oregon. It differs from all other members of the genus in hosts, geographic location, and in possessing pre- and para-ovarian, as well as post-ovarian testes. Diagnosis of Diclidophora (Yamaguti 1963) is emended.", "contents": "Diclidophora embiotocae sp. n. (Monogenea, Diclidophoridae), a parasite of embiotocid fishes of Oregon. Diclidophora embiotocae sp. n. is described from adult specimens obtained from the gills of three species of embiotocid fishes from the central coast of Oregon. It differs from all other members of the genus in hosts, geographic location, and in possessing pre- and para-ovarian, as well as post-ovarian testes. Diagnosis of Diclidophora (Yamaguti 1963) is emended."}
{"id": "PMID:480084", "title": "Bronchogenic cyst.", "content": "Two recent patients with bronchogenic cysts, presenting in an atypical manner, stimulated our review of this subject. Twenty patients with bronchogenic cysts have been treated at the Boston Floating Hospital over the past 20 yr. Of these patients, 19 out of 20 were symptomatic, the most common symptom being fever (6 out of 20). Half of our patients had no respiratory symptoms but only one was found to have an asymptomatic mass on chest x-ray. The majority of theses cysts were found within the pulmonary parenchyma, the right lung being affected three times more commonly than the left. These facts are at odds with the reports in the literature. Three were found in the neck. Bronchogenic cysts are generally thought to be small, solitary, and limited in area. However, a significant number in our series (8 out of 20) were large, multiple or multicystic, and involved segments, lobes, multiple lobes, and, on one occasion, an entire lung. The correct pre-operative diagnosis was made in only 30% of the cases. The fact that the diagnosis was often missed led to long delays in treatment resulting in recurrent infectious complications and repeated hospitalizations. The most common cause of error in diagnosis was failure to follow a pneumonia to complete resolution. Despite the frequent delays in diagnosis, surgical treatment was curative in all patients.", "contents": "Bronchogenic cyst. Two recent patients with bronchogenic cysts, presenting in an atypical manner, stimulated our review of this subject. Twenty patients with bronchogenic cysts have been treated at the Boston Floating Hospital over the past 20 yr. Of these patients, 19 out of 20 were symptomatic, the most common symptom being fever (6 out of 20). Half of our patients had no respiratory symptoms but only one was found to have an asymptomatic mass on chest x-ray. The majority of theses cysts were found within the pulmonary parenchyma, the right lung being affected three times more commonly than the left. These facts are at odds with the reports in the literature. Three were found in the neck. Bronchogenic cysts are generally thought to be small, solitary, and limited in area. However, a significant number in our series (8 out of 20) were large, multiple or multicystic, and involved segments, lobes, multiple lobes, and, on one occasion, an entire lung. The correct pre-operative diagnosis was made in only 30% of the cases. The fact that the diagnosis was often missed led to long delays in treatment resulting in recurrent infectious complications and repeated hospitalizations. The most common cause of error in diagnosis was failure to follow a pneumonia to complete resolution. Despite the frequent delays in diagnosis, surgical treatment was curative in all patients."}
{"id": "PMID:480085", "title": "Pectus carinatum: results of surgical therapy.", "content": "There are a variety of protrusion defects of the anterior chest which are collectively referred to as pectus carinatum. Over a 14-yr period, 13 patients have had surgical correction of pectus carinatum at The Johns Hopkins Hospital. Technique includes costal cartilage resection and a sternal osteotomy. There was no mortality, complications were few and results are good. Repair is recommended for patients in late adolescence due to the cosmetic nature of the procedure, the apparent lack of an associated postural deformity and the good results of operation in this age group.", "contents": "Pectus carinatum: results of surgical therapy. There are a variety of protrusion defects of the anterior chest which are collectively referred to as pectus carinatum. Over a 14-yr period, 13 patients have had surgical correction of pectus carinatum at The Johns Hopkins Hospital. Technique includes costal cartilage resection and a sternal osteotomy. There was no mortality, complications were few and results are good. Repair is recommended for patients in late adolescence due to the cosmetic nature of the procedure, the apparent lack of an associated postural deformity and the good results of operation in this age group."}
{"id": "PMID:480086", "title": "Tongue lesions in children.", "content": "A review of tongue lesions in children showed that there is a great variety requiring operative intervention, often in infancy. While the presenting symptoms may be related to dysphagia and dyspnea, the aim of operative intervention should not only be to salvage life by restoration of breathing and swallowing, but also to leave a tongue capable of adequate speech, taste, sensation, and normal orofacial development. Intimate knowledge of lingual anatomy and function is necessary to allow selection of the ideal procedure and appropriate timing of the therapy. While careful observation and nonoperative approach may be indicated in non-neoplastic macroglossia, early intervention is often necessary in diffuse neoplastic lesions such as lymphangioma, fibromatosis, or fibrolipomatous dysplasia. While malignant tumors are rare in childhood, they do occur and have to ruled out.", "contents": "Tongue lesions in children. A review of tongue lesions in children showed that there is a great variety requiring operative intervention, often in infancy. While the presenting symptoms may be related to dysphagia and dyspnea, the aim of operative intervention should not only be to salvage life by restoration of breathing and swallowing, but also to leave a tongue capable of adequate speech, taste, sensation, and normal orofacial development. Intimate knowledge of lingual anatomy and function is necessary to allow selection of the ideal procedure and appropriate timing of the therapy. While careful observation and nonoperative approach may be indicated in non-neoplastic macroglossia, early intervention is often necessary in diffuse neoplastic lesions such as lymphangioma, fibromatosis, or fibrolipomatous dysplasia. While malignant tumors are rare in childhood, they do occur and have to ruled out."}
{"id": "PMID:480088", "title": "Pelvic exsanguination following umbilical artery catheterization in neonates.", "content": "Umbilical artery catheterization in 400 newborns was followed by umbilical or hypogastric artery perforation with massive extra- and intraperitoneal bleeding in 7 infants. Common predisposing factors were multiple attempts and insertion against resistance. A characteristic clinical picture developed, including hypotension, abdominal distention, and lower abdominal wall induration and ecchymosis, often extending into the genitalia and upper thighs, with absent femoral pulses. Treatment is immediate transfusion and bilateral umbilical and hypogastric artery ligation. One of seven patients survived.", "contents": "Pelvic exsanguination following umbilical artery catheterization in neonates. Umbilical artery catheterization in 400 newborns was followed by umbilical or hypogastric artery perforation with massive extra- and intraperitoneal bleeding in 7 infants. Common predisposing factors were multiple attempts and insertion against resistance. A characteristic clinical picture developed, including hypotension, abdominal distention, and lower abdominal wall induration and ecchymosis, often extending into the genitalia and upper thighs, with absent femoral pulses. Treatment is immediate transfusion and bilateral umbilical and hypogastric artery ligation. One of seven patients survived."}
{"id": "PMID:480090", "title": "Mixed gonadal dysgenesis, pathogensis, and management.", "content": "Fourteen patients with Mixed Gonadal Dysgenesis who presented as infants or children are discussed. Gonadal asymmetry, and/or sex chromosomal mosaicism, as well as retained Mullerian Ducts characterize the anomaly. The dysgenetic testis may occur as the result of a cascade of development mishaps stemming from abnormalities of H-Y antigen expression or function that lead to abnormal differentiation of the indifferent urogenital ridge and, in turn, to aberrant production of Mullerian inhibiting Substance and testosterone. The latter two cause retention of Mullerian ducts and incomplete masculinization of the external genitalia. Absence of a second X chromosome may lead to the formation of a streak ovary, in which the dysgenetic testis may invoke formation of hilar and medullary cords. Neoplastic transformation, so characteristic of this group of patients, may result from unprotected germ cells and abnormally high and prolonged gonadotropin stimulation. Gonadoblastoma and seminoma-dysgerminomas are the tumors found in the gonads with the risk exceeding 50% as the third decade is approached. Laterality of the gonads in this anomaly remains an enigma. The gonads should be removed at birth if possible and the external genitalia repaired soon thereafter. These patients should be raised as females. The risk of neoplastic transformations must be considered at all stages of management.", "contents": "Mixed gonadal dysgenesis, pathogensis, and management. Fourteen patients with Mixed Gonadal Dysgenesis who presented as infants or children are discussed. Gonadal asymmetry, and/or sex chromosomal mosaicism, as well as retained Mullerian Ducts characterize the anomaly. The dysgenetic testis may occur as the result of a cascade of development mishaps stemming from abnormalities of H-Y antigen expression or function that lead to abnormal differentiation of the indifferent urogenital ridge and, in turn, to aberrant production of Mullerian inhibiting Substance and testosterone. The latter two cause retention of Mullerian ducts and incomplete masculinization of the external genitalia. Absence of a second X chromosome may lead to the formation of a streak ovary, in which the dysgenetic testis may invoke formation of hilar and medullary cords. Neoplastic transformation, so characteristic of this group of patients, may result from unprotected germ cells and abnormally high and prolonged gonadotropin stimulation. Gonadoblastoma and seminoma-dysgerminomas are the tumors found in the gonads with the risk exceeding 50% as the third decade is approached. Laterality of the gonads in this anomaly remains an enigma. The gonads should be removed at birth if possible and the external genitalia repaired soon thereafter. These patients should be raised as females. The risk of neoplastic transformations must be considered at all stages of management."}
{"id": "PMID:480091", "title": "Hepatic portocholecystostomy for biliary atresia.", "content": "Four infants with biliary atresia had gross obliteration of the common hepatic duct but residual patency of the gallbladder, cystic duct and common bile duct. The patients were treated by hepatic portocholecystostomy utilizing the extant bile ducts for biliary reconstruction. Bile drainage was achieved in all four infants. There was a conspicuous absence of postoperative cholangitis. Subsequent obstruction of the distal ducts in two patients necessitated reoperation and construction of a standard biliointestinal conduit. The other two children are surviving, jaundice-free, 5 1/2 and 5 years after operation with minimal sequelae of biliary atresia. Hepatic portocholecystostomy is a feasible surgical alternative to intestinal reconstruction in patients with biliary atresia in whom the disease is limited to the proximal extrahepatic bile ducts.", "contents": "Hepatic portocholecystostomy for biliary atresia. Four infants with biliary atresia had gross obliteration of the common hepatic duct but residual patency of the gallbladder, cystic duct and common bile duct. The patients were treated by hepatic portocholecystostomy utilizing the extant bile ducts for biliary reconstruction. Bile drainage was achieved in all four infants. There was a conspicuous absence of postoperative cholangitis. Subsequent obstruction of the distal ducts in two patients necessitated reoperation and construction of a standard biliointestinal conduit. The other two children are surviving, jaundice-free, 5 1/2 and 5 years after operation with minimal sequelae of biliary atresia. Hepatic portocholecystostomy is a feasible surgical alternative to intestinal reconstruction in patients with biliary atresia in whom the disease is limited to the proximal extrahepatic bile ducts."}
{"id": "PMID:480093", "title": "Determinants of survival after Kasai's operation for biliary atresia using actuarial analysis.", "content": "Survival after Kasai's operation for \"noncorrectable\" biliary atresia is influenced by (1) age, (2) large bile ducts, and (3) concentration of the bile bilirubin. Critical values are: age less than 10.5 wk, any evidence of a large bile duct, and a bile bilirubin concentration greater than 8.8 mg/100 ml. Using these 3 factors, a predictive model is able to identify a favorable group with an 89% expected 4 yr survival.", "contents": "Determinants of survival after Kasai's operation for biliary atresia using actuarial analysis. Survival after Kasai's operation for \"noncorrectable\" biliary atresia is influenced by (1) age, (2) large bile ducts, and (3) concentration of the bile bilirubin. Critical values are: age less than 10.5 wk, any evidence of a large bile duct, and a bile bilirubin concentration greater than 8.8 mg/100 ml. Using these 3 factors, a predictive model is able to identify a favorable group with an 89% expected 4 yr survival."}
{"id": "PMID:480094", "title": "Anatomic observations and etiologic and surgical considerations in choledochal cyst.", "content": "Twenty-five operative and postoperative cholangiograms in children with choledochal cysts indicated an intimate relationship between these cysts and anomalous pancreatico-biliary ductal junction. In all of these patients the distal main pancreatic duct (MPD) is excluded from the sphinctor of Oddi mechanism and free reflux of pancreatic juice up the biliary tree occurs. This leads to chronic cholangitis, which is presumed to be responsible for the formation of the choledochal cysts. Abrupt and intense exposure of the common bile duct (CBD) to pancreatic reflux may cause perforation and bile peritonitis. Long-standing exposure may induce malignant transformation in the choledochal cyst. Total cyst excision and separation of the biliary system from exposure to pancreatic reflux is suggested as the procedure of choice. Long-term results and metabolic and digestive consequences of this operation are yet to be determined.", "contents": "Anatomic observations and etiologic and surgical considerations in choledochal cyst. Twenty-five operative and postoperative cholangiograms in children with choledochal cysts indicated an intimate relationship between these cysts and anomalous pancreatico-biliary ductal junction. In all of these patients the distal main pancreatic duct (MPD) is excluded from the sphinctor of Oddi mechanism and free reflux of pancreatic juice up the biliary tree occurs. This leads to chronic cholangitis, which is presumed to be responsible for the formation of the choledochal cysts. Abrupt and intense exposure of the common bile duct (CBD) to pancreatic reflux may cause perforation and bile peritonitis. Long-standing exposure may induce malignant transformation in the choledochal cyst. Total cyst excision and separation of the biliary system from exposure to pancreatic reflux is suggested as the procedure of choice. Long-term results and metabolic and digestive consequences of this operation are yet to be determined."}
{"id": "PMID:480096", "title": "Crohn's disease in children and adolescents: is inadequate weight gain a valid indication for surgery?", "content": "Seventy children and adolescents hospitalized for Crohn's disease at the University of Michigan Medical Center between 1966 and 1976 are reviewed. Thirty-three of these children were managed medically and 37 underwent a total of 60 operative procedures. There were 33 bowel resections, 7 appendectomies, 4 abscess drainages, 3 bowel bypasses, and 13 miscellaneous operative procedures. The total operative mortality was 1.7%, and there were 10 complications, all of which were successfully managed. Among the 26 children undergoing bowel resections, 22 were below the third percentile for weight preoperatively. Nearly two-thirds of these gained weight to the 35th percentile following resection. The remainder of the children had a variety of growth and symptomatic responses to surgery. Resection aimed at complete removal of diseased bowel can be expected to yield substantial weight gain in children with Crohn's disease.", "contents": "Crohn's disease in children and adolescents: is inadequate weight gain a valid indication for surgery? Seventy children and adolescents hospitalized for Crohn's disease at the University of Michigan Medical Center between 1966 and 1976 are reviewed. Thirty-three of these children were managed medically and 37 underwent a total of 60 operative procedures. There were 33 bowel resections, 7 appendectomies, 4 abscess drainages, 3 bowel bypasses, and 13 miscellaneous operative procedures. The total operative mortality was 1.7%, and there were 10 complications, all of which were successfully managed. Among the 26 children undergoing bowel resections, 22 were below the third percentile for weight preoperatively. Nearly two-thirds of these gained weight to the 35th percentile following resection. The remainder of the children had a variety of growth and symptomatic responses to surgery. Resection aimed at complete removal of diseased bowel can be expected to yield substantial weight gain in children with Crohn's disease."}
{"id": "PMID:480097", "title": "Liver abscess.", "content": "A liver abscess may occur secondary to an umbilical vein catheterization or infection in other parts of the body, and is associated with immune deficiencies, especially chronic granulomatous disease. There are no specific signs and symptoms, but an unexplained fever with upper abdominal tenderness and an enlarged liver were present in all of our cases. A liver-spleen scan is the most useful diagnostic test, demonstrating a filling defect in the liver. A single abscess may be unroofed and drained. When multiple abscesses are found, as many as possible are drained, but long-term specific antibiotic therapy is the most important treatment.", "contents": "Liver abscess. A liver abscess may occur secondary to an umbilical vein catheterization or infection in other parts of the body, and is associated with immune deficiencies, especially chronic granulomatous disease. There are no specific signs and symptoms, but an unexplained fever with upper abdominal tenderness and an enlarged liver were present in all of our cases. A liver-spleen scan is the most useful diagnostic test, demonstrating a filling defect in the liver. A single abscess may be unroofed and drained. When multiple abscesses are found, as many as possible are drained, but long-term specific antibiotic therapy is the most important treatment."}
{"id": "PMID:480098", "title": "Long-term experience with childhood thyroid carcinoma.", "content": "Twenty patients with well-differentiated thyroid carcinoma, 20 yr of age or younger have been treated at Vanderbilt University Hospital. The young patient with thyroid carcinoma frequently presents with far-advanced disease but enjoys an improved survival. Preoperative scintiscanning is useful in the evaluation of patients with childhood thyroid carcinoma. A new noninvasive scan is described and appears to be promising in the preoperative differentiation between benign and malignant thyroid lesions in children and young adults. There is a high instance of nodal metastases, but long-term follow-up would indicate that lobectomy and modified neck dissection are sufficient for most patients. However, total thyroidectomy can be performed safely in those patients with disease in both lobes. Additionalally, frozen section diagnoses were found to be helpful in diagnosing and staging thyroid carcinoma.", "contents": "Long-term experience with childhood thyroid carcinoma. Twenty patients with well-differentiated thyroid carcinoma, 20 yr of age or younger have been treated at Vanderbilt University Hospital. The young patient with thyroid carcinoma frequently presents with far-advanced disease but enjoys an improved survival. Preoperative scintiscanning is useful in the evaluation of patients with childhood thyroid carcinoma. A new noninvasive scan is described and appears to be promising in the preoperative differentiation between benign and malignant thyroid lesions in children and young adults. There is a high instance of nodal metastases, but long-term follow-up would indicate that lobectomy and modified neck dissection are sufficient for most patients. However, total thyroidectomy can be performed safely in those patients with disease in both lobes. Additionalally, frozen section diagnoses were found to be helpful in diagnosing and staging thyroid carcinoma."}
{"id": "PMID:480099", "title": "Partial gastric wrap-around as an alternative procedure in the treatment of hiatal hernia.", "content": "The Allison, Belsey, and Nissen antireflux operations have been widely used over the last 15 yr. A number of problems have followed from these procedures that have prompted us under certain circumstances to seek alternative operations. In the last 2 yr, in selected patients, we have done a partial gastric wrap-around (Dor-Nissen) operation on 16 children from age 2 wk to 13 yr for the following reasons: failed Allison and Nissen repairs, two infants with such a small stomach that only a partial stomach wrap-around was possible, with a Heller cardiomyotomy, and for reflux with or without an accompanying hiatal hernia. All of these Dor-Nissen partial wrap-around operations were technically easy to do and all the postoperative patients have been problem-free both clinically and radiologically. Moreover, the gass bloat syndrome seen with the Nissen fundoplication has not been encountered. We recommended this partial wrap-around antireflux operation as a primary procedure, an alterative to one of the more widely used operations, or when re-operating for recurrence of gastroesophageal reflux.", "contents": "Partial gastric wrap-around as an alternative procedure in the treatment of hiatal hernia. The Allison, Belsey, and Nissen antireflux operations have been widely used over the last 15 yr. A number of problems have followed from these procedures that have prompted us under certain circumstances to seek alternative operations. In the last 2 yr, in selected patients, we have done a partial gastric wrap-around (Dor-Nissen) operation on 16 children from age 2 wk to 13 yr for the following reasons: failed Allison and Nissen repairs, two infants with such a small stomach that only a partial stomach wrap-around was possible, with a Heller cardiomyotomy, and for reflux with or without an accompanying hiatal hernia. All of these Dor-Nissen partial wrap-around operations were technically easy to do and all the postoperative patients have been problem-free both clinically and radiologically. Moreover, the gass bloat syndrome seen with the Nissen fundoplication has not been encountered. We recommended this partial wrap-around antireflux operation as a primary procedure, an alterative to one of the more widely used operations, or when re-operating for recurrence of gastroesophageal reflux."}
{"id": "PMID:480100", "title": "Nectotizing enterocolitis: is there a place for resection and primary anatomosis?", "content": "While most situations of peritonitis, perforation and compromised blood flow in necrotizing enterocolitis are best handled by resection and stoma formation, primary resection and anastomosis should be considered in selected cases. Nine premature infants with NEC underwent bowel resection with primary anastomosis in the face of localized perforation and peritonitis. Eight survived and had no problems when oral alimentation was started. One patient with small bowel involvement underwent two anastomoses rather than a high jejunostomy; the proximal anastomosis leaked and led to the death of the patient.", "contents": "Nectotizing enterocolitis: is there a place for resection and primary anatomosis? While most situations of peritonitis, perforation and compromised blood flow in necrotizing enterocolitis are best handled by resection and stoma formation, primary resection and anastomosis should be considered in selected cases. Nine premature infants with NEC underwent bowel resection with primary anastomosis in the face of localized perforation and peritonitis. Eight survived and had no problems when oral alimentation was started. One patient with small bowel involvement underwent two anastomoses rather than a high jejunostomy; the proximal anastomosis leaked and led to the death of the patient."}
{"id": "PMID:480101", "title": "Prospective application of an index of neonatal necrotizing enterocolitis.", "content": "Fifty-five patients were scored 1 to 3 according to the criteria: the character of stools, abdominal findings on palpation, x-ray evidence of pneumatosis intestinalis, the development of pulmonary insufficiency, and the duration of symptoms to positive x-rays. Fifteen patients with scores of less than five were considered to have subclinical NEC with one late death. Twenty-nine of 30 patients with scores of 5-10 responded to medical management with 2 deaths related to recurrent bouts of sepsis without recurrent NEC. Eleven patients required surgery with index scores of 10-14 with 6 deaths occurring uniformly in those patients with scores of 12 or more. Two patients were scored inappropriately low due to the lack of the passage of a stool for analysis. One patient with a score of 4 did not pass a stool but had the other diagnostic criteria for the single false negative of the series. This index correctly determined the severity of NEC of 53 of 55 patients, identified the patients who required surgical intervention and predicted survival.", "contents": "Prospective application of an index of neonatal necrotizing enterocolitis. Fifty-five patients were scored 1 to 3 according to the criteria: the character of stools, abdominal findings on palpation, x-ray evidence of pneumatosis intestinalis, the development of pulmonary insufficiency, and the duration of symptoms to positive x-rays. Fifteen patients with scores of less than five were considered to have subclinical NEC with one late death. Twenty-nine of 30 patients with scores of 5-10 responded to medical management with 2 deaths related to recurrent bouts of sepsis without recurrent NEC. Eleven patients required surgery with index scores of 10-14 with 6 deaths occurring uniformly in those patients with scores of 12 or more. Two patients were scored inappropriately low due to the lack of the passage of a stool for analysis. One patient with a score of 4 did not pass a stool but had the other diagnostic criteria for the single false negative of the series. This index correctly determined the severity of NEC of 53 of 55 patients, identified the patients who required surgical intervention and predicted survival."}
{"id": "PMID:480107", "title": "A comparison of Rorschach developmental level and form-level systems as indicators of psychosis.", "content": "Psychotic and nonpsychotic psychiatric inpatients were tested on the Rorschach. \"Reality-testing\" was measured by four form-level scoring systems designed by Beck, Mayman, Becker, and Wilensky. The psychotic subjects were dichotomized on three diagnostic dimensions: schizophrenia, paranoia, and premorbid social adjustment. No significant differences were found for the Beck system. With the other systems, psychotics showed significantly poorer reality testing than nonpsychotics. No differences were found for the schizophrenic and paranoid dimensions. The Phillips premorbid adjustment score was negatively correlated with all four measures of \"reality-testing.\" The conclusion drawn was that Rorschach measures of \"reality testing\" are associated with psychotic and poor premorbid functioning, but do not differentiate schizophrenic from nonschizophrenic psychotics or paranoid schizophrenics from nonparanoid schizophrenics.", "contents": "A comparison of Rorschach developmental level and form-level systems as indicators of psychosis. Psychotic and nonpsychotic psychiatric inpatients were tested on the Rorschach. \"Reality-testing\" was measured by four form-level scoring systems designed by Beck, Mayman, Becker, and Wilensky. The psychotic subjects were dichotomized on three diagnostic dimensions: schizophrenia, paranoia, and premorbid social adjustment. No significant differences were found for the Beck system. With the other systems, psychotics showed significantly poorer reality testing than nonpsychotics. No differences were found for the schizophrenic and paranoid dimensions. The Phillips premorbid adjustment score was negatively correlated with all four measures of \"reality-testing.\" The conclusion drawn was that Rorschach measures of \"reality testing\" are associated with psychotic and poor premorbid functioning, but do not differentiate schizophrenic from nonschizophrenic psychotics or paranoid schizophrenics from nonparanoid schizophrenics."}
{"id": "PMID:480108", "title": "On the use of symmetry in the Rorschach Test.", "content": "Rorschach's justifications for the use of symmetry in his inkblots are evaluated in the light of recent empirical research concerning the perception of symmetry. The role of symmetry in response facilitation, in the production of whole and movement responses, in the creation of similar conditions for left- and right-handers, and as a response determinant, is discussed and re-evaluated.", "contents": "On the use of symmetry in the Rorschach Test. Rorschach's justifications for the use of symmetry in his inkblots are evaluated in the light of recent empirical research concerning the perception of symmetry. The role of symmetry in response facilitation, in the production of whole and movement responses, in the creation of similar conditions for left- and right-handers, and as a response determinant, is discussed and re-evaluated."}
{"id": "PMID:480109", "title": "Discriminative effectiveness of the Senior Apperception Test with impaired and nonimpaired elderly persons.", "content": "A means was devised of assessing elderly individuals' responses to the Senior Apperception Test (SAT) that would discriminate emotionally-cognitively impaired from nonimpaired. With a matched sample of known impaired and nonimpaired nursing home residents, SAT protocols were scored on four dimensions, and the best discriminating cut-off scores established. Protocols from a second similar matched sample were tested for discriminative effectiveness of previously established criterion scores. Significant discrimination at .01 or better was obtained for all four dimensions in the predicted direction. False positives and false negatives ranged from 7 to 33%. The best predictor score was Interpersonal-relationships, the poorest was mood. This procedure with the SAT appears to be an effective screening method for impairment, and it provides additional useful clinical information.", "contents": "Discriminative effectiveness of the Senior Apperception Test with impaired and nonimpaired elderly persons. A means was devised of assessing elderly individuals' responses to the Senior Apperception Test (SAT) that would discriminate emotionally-cognitively impaired from nonimpaired. With a matched sample of known impaired and nonimpaired nursing home residents, SAT protocols were scored on four dimensions, and the best discriminating cut-off scores established. Protocols from a second similar matched sample were tested for discriminative effectiveness of previously established criterion scores. Significant discrimination at .01 or better was obtained for all four dimensions in the predicted direction. False positives and false negatives ranged from 7 to 33%. The best predictor score was Interpersonal-relationships, the poorest was mood. This procedure with the SAT appears to be an effective screening method for impairment, and it provides additional useful clinical information."}
{"id": "PMID:480110", "title": "Bender-Gestalt emotional indicators and acting-out behavior in young children.", "content": "This study was designed to investigate the relationship between 15 emotional indicators on the Bender-Gestalt Test and acting-out behavior in young children. The subjects were 93 children ranging in age from five to 12 years. Each was administered the Bender. A measure of each subject's overt acting-out behavior was then obtained by having teachers rate each student on a Behavioral Rating Scale. Subjects' records were then divided into groups on the basis of both sex and age. Results indicated that neither the total number of Bender indicators nor any of the individual Bender indicators were correlated significantly with total scores on the rating scale. Use of the Bender as a projective device to measure acting-out behavior was seriously questioned.", "contents": "Bender-Gestalt emotional indicators and acting-out behavior in young children. This study was designed to investigate the relationship between 15 emotional indicators on the Bender-Gestalt Test and acting-out behavior in young children. The subjects were 93 children ranging in age from five to 12 years. Each was administered the Bender. A measure of each subject's overt acting-out behavior was then obtained by having teachers rate each student on a Behavioral Rating Scale. Subjects' records were then divided into groups on the basis of both sex and age. Results indicated that neither the total number of Bender indicators nor any of the individual Bender indicators were correlated significantly with total scores on the rating scale. Use of the Bender as a projective device to measure acting-out behavior was seriously questioned."}
{"id": "PMID:480111", "title": "Hand Test indices of violent and destructive behavior for institutionalized mental retardates.", "content": "The Hand Test (Wagner, 1962) and the maladaptive behavior scale of the AAMD Adaptive Behavior Scale (ABS) were administered to 78 institutionalized retardates (46 males and 32 females) in order to investigate the relationship between violent and destructive behavior and two purported indicants of violent and destructive acting-out behavior on the Hand Test, the acting-out-score (AOS and movement-content response (ACT-MOV). Significant correlations between the two Hand Test indices and the number of types of violent and destructive behavior evinced during a three-month period were obtained.", "contents": "Hand Test indices of violent and destructive behavior for institutionalized mental retardates. The Hand Test (Wagner, 1962) and the maladaptive behavior scale of the AAMD Adaptive Behavior Scale (ABS) were administered to 78 institutionalized retardates (46 males and 32 females) in order to investigate the relationship between violent and destructive behavior and two purported indicants of violent and destructive acting-out behavior on the Hand Test, the acting-out-score (AOS and movement-content response (ACT-MOV). Significant correlations between the two Hand Test indices and the number of types of violent and destructive behavior evinced during a three-month period were obtained."}
{"id": "PMID:480112", "title": "The MMPI as a predictor of early termination from polydrug abuse treatment.", "content": "The MMPI protocols obtained upon admission to an inpatient program for treatment of polydrug abuse were studied to determine if they could identify abusers who terminated prematurely from treatment. Individual scale T scores, profile code types and discriminant scores were evaluated for 104 patients who completed treatment (\"stayers\") and 72 patients who left prematurely (\"quitters\"). Although quitters and stayers did not differentiate on primary drug of abuse, age, sex, race, or IQ, quitters were less likely to obtain normal limits or neurotic code profiles, Univariate analyses revealed that quitters obtained higher mean elevations on 6 of the 15 scales studied (F, 4/Pd, 6/Pa, 8/Sc, 9/Ma, A). Discriminant scores based on a stepwise discriminant analysis correctly identified 71% of the study sample, representing a 13.7% improvement over base rate prediction. The probable importance of initial level of psychological discomfort and ability to delay gratification, and the need to examine varied treatment modalities for polydrug abusers identified as at risk for premature termination from treatment were explored.", "contents": "The MMPI as a predictor of early termination from polydrug abuse treatment. The MMPI protocols obtained upon admission to an inpatient program for treatment of polydrug abuse were studied to determine if they could identify abusers who terminated prematurely from treatment. Individual scale T scores, profile code types and discriminant scores were evaluated for 104 patients who completed treatment (\"stayers\") and 72 patients who left prematurely (\"quitters\"). Although quitters and stayers did not differentiate on primary drug of abuse, age, sex, race, or IQ, quitters were less likely to obtain normal limits or neurotic code profiles, Univariate analyses revealed that quitters obtained higher mean elevations on 6 of the 15 scales studied (F, 4/Pd, 6/Pa, 8/Sc, 9/Ma, A). Discriminant scores based on a stepwise discriminant analysis correctly identified 71% of the study sample, representing a 13.7% improvement over base rate prediction. The probable importance of initial level of psychological discomfort and ability to delay gratification, and the need to examine varied treatment modalities for polydrug abusers identified as at risk for premature termination from treatment were explored."}
{"id": "PMID:480113", "title": "The structure of death anxiety: a factor analytic study.", "content": "University students in Canada (n = 255) and Northern Ireland (n = 315), graduate nursing students (n = 124), funeral service students (n = 79), and members of the Unitarian Fellowship (n = 83) completed Templer's Death Anxiety Scale (DAS). The responses of each group were subjected to principal components factor analysis using varimax rotational procedures and the factor scores derived from this analysis were then contrasted using multiple discriminant function analysis. Results indicated that there was more commonality than uniqueness in the factor patterns for these groups. Four common death anxiety patterns were classified as follows: (a) cognitive-affective concerns; (b) concern about physical alterations; (c) concern about the passage of time; and (d) concern about stressors and pain. These factors are consistent with and extend the views expressed in previous research (i.e., Pandy, 1974-75; Pandy & Templer, 1972.", "contents": "The structure of death anxiety: a factor analytic study. University students in Canada (n = 255) and Northern Ireland (n = 315), graduate nursing students (n = 124), funeral service students (n = 79), and members of the Unitarian Fellowship (n = 83) completed Templer's Death Anxiety Scale (DAS). The responses of each group were subjected to principal components factor analysis using varimax rotational procedures and the factor scores derived from this analysis were then contrasted using multiple discriminant function analysis. Results indicated that there was more commonality than uniqueness in the factor patterns for these groups. Four common death anxiety patterns were classified as follows: (a) cognitive-affective concerns; (b) concern about physical alterations; (c) concern about the passage of time; and (d) concern about stressors and pain. These factors are consistent with and extend the views expressed in previous research (i.e., Pandy, 1974-75; Pandy & Templer, 1972."}
{"id": "PMID:480114", "title": "Assertiveness, locus of control and coping behaviors used to diminish tension.", "content": "To investigate how coping behaviors may be preconditioned by assertiveness and locus of control, a questionnaire was given to 133 undergraduates of whom 87% reported recent tension attributed mainly to academic pressures. From a list of 22 common coping reactions, the students were asked to check those they use to deal with their tensions. The coping responses were also correlated with scores on an assertiveness inventory (AES) and Rotter's Internal-External Locus of Control (I-E) Scale. Reliance on one's own initiatives and turning to significant others were the responses most frequently reported as reactions to tension while seeking professional help was ranked last. Male students more frequently reported using marijuana or seeking sexual comfort than females, while females more frequently reported dysfunctional reactions such as spending endless hours thinking about the problem. The AES correlated significantly with problem-solving approaches. The IE Scale showed internals more inclined to meditate; externals indicated a greater likelihood to seek professional help, fantasize, or drink alcohol.", "contents": "Assertiveness, locus of control and coping behaviors used to diminish tension. To investigate how coping behaviors may be preconditioned by assertiveness and locus of control, a questionnaire was given to 133 undergraduates of whom 87% reported recent tension attributed mainly to academic pressures. From a list of 22 common coping reactions, the students were asked to check those they use to deal with their tensions. The coping responses were also correlated with scores on an assertiveness inventory (AES) and Rotter's Internal-External Locus of Control (I-E) Scale. Reliance on one's own initiatives and turning to significant others were the responses most frequently reported as reactions to tension while seeking professional help was ranked last. Male students more frequently reported using marijuana or seeking sexual comfort than females, while females more frequently reported dysfunctional reactions such as spending endless hours thinking about the problem. The AES correlated significantly with problem-solving approaches. The IE Scale showed internals more inclined to meditate; externals indicated a greater likelihood to seek professional help, fantasize, or drink alcohol."}
{"id": "PMID:480146", "title": "Bioavailability of imipramine tablets relative to a stable isotope-labeled internal standard: increasing the power of bioavailability tests.", "content": "A new methodology for comparative bioavailability testing is described in which each drug formulation is compared with a stable isotope-labeled variant of the drug that is consumed orally in solution at the same time the tested formulation is ingested. The methodology is used to determine the comparative bioavailabilities of two commercially available brands of imipramine hydrochloride. The power of the new methodology to detect differences between drug formulations, when, in fact, such differences exist, is shown to be superior to that of conventional bioavailability tests.", "contents": "Bioavailability of imipramine tablets relative to a stable isotope-labeled internal standard: increasing the power of bioavailability tests. A new methodology for comparative bioavailability testing is described in which each drug formulation is compared with a stable isotope-labeled variant of the drug that is consumed orally in solution at the same time the tested formulation is ingested. The methodology is used to determine the comparative bioavailabilities of two commercially available brands of imipramine hydrochloride. The power of the new methodology to detect differences between drug formulations, when, in fact, such differences exist, is shown to be superior to that of conventional bioavailability tests."}
{"id": "PMID:480147", "title": "Disposition of synethetic glucocorticoids. I. Pharmacokinetics of dexamethasone in healthy adults.", "content": "The pharmacokinetics of dexamethasone alcohol is described in six male and six female healthy adult volunteers who each received 8 mg of dexamethasone phosphate by bolus intravenous injection. Quantitation of the alcohol was done using a high-performance liquid chromatographic method with improved specificity. Statistical evaluation of the results generated by nonlinear least-squares regression analysis of the plasma concentration-time data shows that the phosphate ester is very rapidly hydrolyzed to the alcohol and a biexponential equation is the simplest polyexponential equation that is consistent with the data. The terminal phase half-life t1/2 beta was significantly greater (P less than 0.05) in males (mean 201.5 min) than in females (mean 142.3 min). They prolonged t1/2 beta in males did not appear to be caused by an impaired capacity to eliminate dexamethasone since the total plasma clearance did not differ between males (mean 24.5 ml/min) and females (mean 242.9 ml/min). There was, however, a high positive correlation between t1/2 beta and Vdss among the 12 adults (r = 0.92, p less than 0.001). There were also significant correlation between Vdss and body weight (r = 0.67, p less than 0.05) and t1/2 beta and body weight (r = 0.80, p less than 0.01). The difference in body weight between the sexes seems to be the main factor contributing to the difference observed in t1/2 beta. An average of only 2.6% of the dose was found unchanged in a 24-hr urine sample, and hence it appears that dexamethasone is primarily eliminated by extrarenal, probably hepatic, mechanisms.", "contents": "Disposition of synethetic glucocorticoids. I. Pharmacokinetics of dexamethasone in healthy adults. The pharmacokinetics of dexamethasone alcohol is described in six male and six female healthy adult volunteers who each received 8 mg of dexamethasone phosphate by bolus intravenous injection. Quantitation of the alcohol was done using a high-performance liquid chromatographic method with improved specificity. Statistical evaluation of the results generated by nonlinear least-squares regression analysis of the plasma concentration-time data shows that the phosphate ester is very rapidly hydrolyzed to the alcohol and a biexponential equation is the simplest polyexponential equation that is consistent with the data. The terminal phase half-life t1/2 beta was significantly greater (P less than 0.05) in males (mean 201.5 min) than in females (mean 142.3 min). They prolonged t1/2 beta in males did not appear to be caused by an impaired capacity to eliminate dexamethasone since the total plasma clearance did not differ between males (mean 24.5 ml/min) and females (mean 242.9 ml/min). There was, however, a high positive correlation between t1/2 beta and Vdss among the 12 adults (r = 0.92, p less than 0.001). There were also significant correlation between Vdss and body weight (r = 0.67, p less than 0.05) and t1/2 beta and body weight (r = 0.80, p less than 0.01). The difference in body weight between the sexes seems to be the main factor contributing to the difference observed in t1/2 beta. An average of only 2.6% of the dose was found unchanged in a 24-hr urine sample, and hence it appears that dexamethasone is primarily eliminated by extrarenal, probably hepatic, mechanisms."}
{"id": "PMID:480148", "title": "Attenuation of furosemide's diuretic effect by indomethacin: pharmacokinetic evaluation.", "content": "The pharmacokinetics and pharmacodynamics of intravenous furosemide, 40 mg, were studied in four normal males in a crossover fashion with and without indomethacin pretreatment. In each study 16 plasma and 10 urine samples were collected over 24 hr. Fluid and electrolyte urinary losses were replaced orally throughout the study. Unchanged furosemide and indomethacin were measured using HPLC; urinary sodium was measured by flame photometry. Pretreatment with indomethacin resulted in increased and prolonged fourosemide plasma levels, increased area under the curve, decreased plasma clearance, decreased renal clearance, increased half-life, no change in volume of distribution, and decreased sodium excretion and urine volume. Analysis of sodium excretion rate with time shows that the inhibiting effect of indomethacin was greater during the first 2 hr than at later times.", "contents": "Attenuation of furosemide's diuretic effect by indomethacin: pharmacokinetic evaluation. The pharmacokinetics and pharmacodynamics of intravenous furosemide, 40 mg, were studied in four normal males in a crossover fashion with and without indomethacin pretreatment. In each study 16 plasma and 10 urine samples were collected over 24 hr. Fluid and electrolyte urinary losses were replaced orally throughout the study. Unchanged furosemide and indomethacin were measured using HPLC; urinary sodium was measured by flame photometry. Pretreatment with indomethacin resulted in increased and prolonged fourosemide plasma levels, increased area under the curve, decreased plasma clearance, decreased renal clearance, increased half-life, no change in volume of distribution, and decreased sodium excretion and urine volume. Analysis of sodium excretion rate with time shows that the inhibiting effect of indomethacin was greater during the first 2 hr than at later times."}
{"id": "PMID:480149", "title": "Sequential first-pass elimination of a metabolite derived from a precursor.", "content": "We examined data from our previous studies in which we not only delivered perfusate containing tracer concentrations of [14C]phenacetin and its metabolite [3H]acetaminophen under constant perfusate flow (10 ml/min/liver) into the rat liver preparation just once, but also recirculated fresh reservoir perfusate containing a tracer dose of [14C]phenacetin through the same rat liver preparation. From the single-pass studies, estimates of fm, the fractional rate of conversion for [14C]phenacetin to form [14C]acetaminophen, and F(M.P), the apparent availability of [14C]acetaminophen, were obtained by determining the concentrations of [14C]acetaminophen in the perfusate before and after incubation with Glusulase. These estimates were fm = 0.871 +/- 0.16 and F(M.P) = 0.43 +/- 0.10. These and the steady-state clearance values of phenacetin (9.1 +/- 0.8 ml/min) and acetaminophen (6.7 +/- 0.7 ml/min) from the single-pass studies were used to predict the concentrations of [14C]acetaminophen in the reservoir perfusate on recirculation of [14C]phenacetin. We found that the sequential first-pass elimination of the metabolite must be considered when the metabolite is highly extracted by the liver. If we had neglected to take this into account, the fractional rate of conversion of a precursor to form a metabolite and the rate of formation of the metabolite would have been underestimated by the factor F(M.P).", "contents": "Sequential first-pass elimination of a metabolite derived from a precursor. We examined data from our previous studies in which we not only delivered perfusate containing tracer concentrations of [14C]phenacetin and its metabolite [3H]acetaminophen under constant perfusate flow (10 ml/min/liver) into the rat liver preparation just once, but also recirculated fresh reservoir perfusate containing a tracer dose of [14C]phenacetin through the same rat liver preparation. From the single-pass studies, estimates of fm, the fractional rate of conversion for [14C]phenacetin to form [14C]acetaminophen, and F(M.P), the apparent availability of [14C]acetaminophen, were obtained by determining the concentrations of [14C]acetaminophen in the perfusate before and after incubation with Glusulase. These estimates were fm = 0.871 +/- 0.16 and F(M.P) = 0.43 +/- 0.10. These and the steady-state clearance values of phenacetin (9.1 +/- 0.8 ml/min) and acetaminophen (6.7 +/- 0.7 ml/min) from the single-pass studies were used to predict the concentrations of [14C]acetaminophen in the reservoir perfusate on recirculation of [14C]phenacetin. We found that the sequential first-pass elimination of the metabolite must be considered when the metabolite is highly extracted by the liver. If we had neglected to take this into account, the fractional rate of conversion of a precursor to form a metabolite and the rate of formation of the metabolite would have been underestimated by the factor F(M.P)."}
{"id": "PMID:480150", "title": "Creatinine kinetics in the rabbit.", "content": "To evaluate the kinetics of creatinine in the rabbit, two radiotagged creatinine forms were used, with 14C in the amidino group or with it in the carboxyl group. Five animals were injected intravenously with tracer amounts, and plasma samples were taken for 250-300 min; urine and feces samples were taken over longer periods. A chromatographic method was used to separate unchanged creatinine from other components of the samples prior to measurement for chemical and radioactivity content. Equations for the proposed flow model were derived and fitted to specific activity data, and values for transfer constants, production rates, and pool sizes were calculated. Evidence supported the concept that a two-compartment model is required and that production occurs in the peripheral compartment. Evidence also indicated that muscle represented a major part of the peripheral compartment. Small amounts of metabolites, chiefly guanido compounds, were detected when amidino-labeled creatinine was used. Also, small amounts of unchanged creatinine and metabilites were found in the feces.", "contents": "Creatinine kinetics in the rabbit. To evaluate the kinetics of creatinine in the rabbit, two radiotagged creatinine forms were used, with 14C in the amidino group or with it in the carboxyl group. Five animals were injected intravenously with tracer amounts, and plasma samples were taken for 250-300 min; urine and feces samples were taken over longer periods. A chromatographic method was used to separate unchanged creatinine from other components of the samples prior to measurement for chemical and radioactivity content. Equations for the proposed flow model were derived and fitted to specific activity data, and values for transfer constants, production rates, and pool sizes were calculated. Evidence supported the concept that a two-compartment model is required and that production occurs in the peripheral compartment. Evidence also indicated that muscle represented a major part of the peripheral compartment. Small amounts of metabolites, chiefly guanido compounds, were detected when amidino-labeled creatinine was used. Also, small amounts of unchanged creatinine and metabilites were found in the feces."}
{"id": "PMID:480151", "title": "Divergence in pharmacokinetic parameters of quinidine obtained by specific and nonspecific assay methods.", "content": "Previously published estimates of pharmacokinetic characteristics of quinidine can be shown to be dependent on whether the investigators have used analytical methods which are specific for quinidine. Areas under the plasma concentration-time curve and peak plasma concentrations after administration of the drug were higher and clearance values consequently lower in studies utilizing nonspecific assays unable to distinguish quinidine from its metabolities. The error introduced is larger after oral administration as a result of marked first-pass metabolism of quinidine. The absolute oral bioavailabilities from pharmaceutical preparations might therefore be estimated higher in studies with assays including metabolites in the determination. Although the pharmacodynamic response to quinidine is related to the plasma concentration, the therapeutic window of drug concentrations has been defined only using nonspecific assays. In light of the availability of newly developed specific assays, redefinition of the range of therapeutic plasma concentrations is opportune.", "contents": "Divergence in pharmacokinetic parameters of quinidine obtained by specific and nonspecific assay methods. Previously published estimates of pharmacokinetic characteristics of quinidine can be shown to be dependent on whether the investigators have used analytical methods which are specific for quinidine. Areas under the plasma concentration-time curve and peak plasma concentrations after administration of the drug were higher and clearance values consequently lower in studies utilizing nonspecific assays unable to distinguish quinidine from its metabolities. The error introduced is larger after oral administration as a result of marked first-pass metabolism of quinidine. The absolute oral bioavailabilities from pharmaceutical preparations might therefore be estimated higher in studies with assays including metabolites in the determination. Although the pharmacodynamic response to quinidine is related to the plasma concentration, the therapeutic window of drug concentrations has been defined only using nonspecific assays. In light of the availability of newly developed specific assays, redefinition of the range of therapeutic plasma concentrations is opportune."}
{"id": "PMID:480152", "title": "Examination of blood clobazam levels and several pupillary measures in humans.", "content": "The State-Trait Anxiety Inventory was administered to 15 subjects before initiation of the experiment. Three subgroups of five subjects were defined by computing the unweighted sum of the state and trait anxiety scores. A 40-mg dose of clobazam, a 1.5-benzodiazepine, was administered to each subject and repeated with two additional dosage forms following a 2-week washout period. Blood samples were withdrawn, and blood levels were determined by fluorometric analysis. Additionally, pupillary measures of critical flicker fusion, constriction, and dilation in response to a cognitive task were obtained at 0, 2, 4, and 6 hr. A repeated measures analysis of variance revealed that blood levels were, as expected, statistically different over time and dosage form. The pupillary constriction mirrored the blood levels in statistical patterns. The pupillary measure of cognition related to the anxiety state after the performance effects of the cognitive task were statistically removed. The results suggest that clobazam has less immediate human effect than does diazepam.", "contents": "Examination of blood clobazam levels and several pupillary measures in humans. The State-Trait Anxiety Inventory was administered to 15 subjects before initiation of the experiment. Three subgroups of five subjects were defined by computing the unweighted sum of the state and trait anxiety scores. A 40-mg dose of clobazam, a 1.5-benzodiazepine, was administered to each subject and repeated with two additional dosage forms following a 2-week washout period. Blood samples were withdrawn, and blood levels were determined by fluorometric analysis. Additionally, pupillary measures of critical flicker fusion, constriction, and dilation in response to a cognitive task were obtained at 0, 2, 4, and 6 hr. A repeated measures analysis of variance revealed that blood levels were, as expected, statistically different over time and dosage form. The pupillary constriction mirrored the blood levels in statistical patterns. The pupillary measure of cognition related to the anxiety state after the performance effects of the cognitive task were statistically removed. The results suggest that clobazam has less immediate human effect than does diazepam."}
{"id": "PMID:480153", "title": "Physical characterization of solid iopanoic acid forms.", "content": "Three solid forms of iopanoic acid were characterized by X-ray diffraction, differential scanning calorimetry, scanning electron microscopy, thermal microscopy, IR spectroscopy, and dissolution studies. X-ray analysis demonstrated that two solid forms were crystalline and that the third was amorphous. The amorphous form had been reported previously as crystalline. Enthalpies and entropies of transition were calculated using differential scanning calorimetry. A fourth form, a benzene solvate, also was isolated but proved to be too unstable at room temperature to permit conclusive characterization. The amorphous form demonstrated a 10-fold greater intrinsic dissolution rate than the commercially available form (Form I). Form II's intrinsic dissolution rate was 1.5 times greater than that of Form I. In powder dissolution studies, the peak solubilities of the different forms followed the same rank order as their intrinsic dissolution rates. Form II was relatively stable in aqueous saturated solutions, but the amorphous form was rapidly converted to Form I under similar conditions.", "contents": "Physical characterization of solid iopanoic acid forms. Three solid forms of iopanoic acid were characterized by X-ray diffraction, differential scanning calorimetry, scanning electron microscopy, thermal microscopy, IR spectroscopy, and dissolution studies. X-ray analysis demonstrated that two solid forms were crystalline and that the third was amorphous. The amorphous form had been reported previously as crystalline. Enthalpies and entropies of transition were calculated using differential scanning calorimetry. A fourth form, a benzene solvate, also was isolated but proved to be too unstable at room temperature to permit conclusive characterization. The amorphous form demonstrated a 10-fold greater intrinsic dissolution rate than the commercially available form (Form I). Form II's intrinsic dissolution rate was 1.5 times greater than that of Form I. In powder dissolution studies, the peak solubilities of the different forms followed the same rank order as their intrinsic dissolution rates. Form II was relatively stable in aqueous saturated solutions, but the amorphous form was rapidly converted to Form I under similar conditions."}
{"id": "PMID:480154", "title": "Guanabenz degradation products and stability assay.", "content": "Guanabenz, [(2,6-dichlorobenzylidene)amino]guanidine, acetate was shown to be the E-isomer. It decomposed to form the Z-isomer, 2,6-dichlorobenzaldehyde, aminoguanidine, and 2,6-dichlorobenzaldehyde semicarbazone. A stability-indicating assay for the intact drug in the presence of all of its decomposition products by the use of UV spectroscopy is presented.", "contents": "Guanabenz degradation products and stability assay. Guanabenz, [(2,6-dichlorobenzylidene)amino]guanidine, acetate was shown to be the E-isomer. It decomposed to form the Z-isomer, 2,6-dichlorobenzaldehyde, aminoguanidine, and 2,6-dichlorobenzaldehyde semicarbazone. A stability-indicating assay for the intact drug in the presence of all of its decomposition products by the use of UV spectroscopy is presented."}
{"id": "PMID:480155", "title": "Pharmacokinetic model for procaine in humans during and following intravenous infusion.", "content": "A multicompartment pharmacokinetic model is presented to describe procaine distribution in humans during and following intravenous infusion. The model, based on a general perfusion model, relates individual characteristics such as sex, age, weight, height, infusion rate and duration, and hematocrit to general parameters such as drug metabolism, protein binding, ion-trapping effects, and tissue-plasma distribution coefficients to provide an individualized distribution prediction. Experimentally observed kinetics of blood procaine levels collected during intravenous procaine infusion as an adjunct to surgical anesthesia and blood lidocaine levels obtained from the literature compared very well with the model simulation.", "contents": "Pharmacokinetic model for procaine in humans during and following intravenous infusion. A multicompartment pharmacokinetic model is presented to describe procaine distribution in humans during and following intravenous infusion. The model, based on a general perfusion model, relates individual characteristics such as sex, age, weight, height, infusion rate and duration, and hematocrit to general parameters such as drug metabolism, protein binding, ion-trapping effects, and tissue-plasma distribution coefficients to provide an individualized distribution prediction. Experimentally observed kinetics of blood procaine levels collected during intravenous procaine infusion as an adjunct to surgical anesthesia and blood lidocaine levels obtained from the literature compared very well with the model simulation."}
{"id": "PMID:480156", "title": "Myoinositol uptake by rat hepatocytes in vitro.", "content": "Myoinositol uptake by rat hepatocytes in vitro was studied. Adult rat hepatocytes were prepared by digestion of the perfused liver with collagenase. Cell suspensions were incubated with tritium-labeled myoinositol in pH 7.4 Krebs bicarbonate solution containing 1% gelatin at 37 degrees. 14C-Carbon-labeled polyethylene glycol was used as a marker of adherent extracellular fluid volume. Myoinositol uptake was demonstrable after 5 min of incubation, but no intracellular concentration in excess of that in the incubation medium was observed after 60 min of incubation. Uptake saturation over a wide myoinositol concentration range could not be demonstrated. Neither the omission of sodium ions nor the inclusion of ouabain suppressed the distribution ratio significantly. Metabolic inhibitors and lower temperatures also showed no effect. Hexoses, phlorizin or mannitol, exerted no observable effect on myoinositol uptake. The results indicated that myoinositol uptake by rat hepatocytes is probably a passive process.", "contents": "Myoinositol uptake by rat hepatocytes in vitro. Myoinositol uptake by rat hepatocytes in vitro was studied. Adult rat hepatocytes were prepared by digestion of the perfused liver with collagenase. Cell suspensions were incubated with tritium-labeled myoinositol in pH 7.4 Krebs bicarbonate solution containing 1% gelatin at 37 degrees. 14C-Carbon-labeled polyethylene glycol was used as a marker of adherent extracellular fluid volume. Myoinositol uptake was demonstrable after 5 min of incubation, but no intracellular concentration in excess of that in the incubation medium was observed after 60 min of incubation. Uptake saturation over a wide myoinositol concentration range could not be demonstrated. Neither the omission of sodium ions nor the inclusion of ouabain suppressed the distribution ratio significantly. Metabolic inhibitors and lower temperatures also showed no effect. Hexoses, phlorizin or mannitol, exerted no observable effect on myoinositol uptake. The results indicated that myoinositol uptake by rat hepatocytes is probably a passive process."}
{"id": "PMID:480157", "title": "Binding of mono- and bis-N-substituted benzoquinolinium halides to butyrylcholinesterase: fluorescence measurements.", "content": "The dissociation constants (Kdiss) of mono-and bis-N-substituted benzoquinolinium salts from butyrylcholinesterase were determined by the disappearance of the fluorescence of the free ligand. The Kdiss value determined by this method agreed closely with the dissociation constants (KI,s) of the free enzyme obtained by steady-state kinetic studies. The results indicate that the N-substituted benzoquinolinium salts with a strong binding ability to butyrylcholinesterase also are potent inhibitors of the esterolytic properties of the enzyme. Enzyme binding is favored by the coplanar structure and does not require a high concentration of charge on the onium function. The shape of the hydrophobic structures markedly influences binding.", "contents": "Binding of mono- and bis-N-substituted benzoquinolinium halides to butyrylcholinesterase: fluorescence measurements. The dissociation constants (Kdiss) of mono-and bis-N-substituted benzoquinolinium salts from butyrylcholinesterase were determined by the disappearance of the fluorescence of the free ligand. The Kdiss value determined by this method agreed closely with the dissociation constants (KI,s) of the free enzyme obtained by steady-state kinetic studies. The results indicate that the N-substituted benzoquinolinium salts with a strong binding ability to butyrylcholinesterase also are potent inhibitors of the esterolytic properties of the enzyme. Enzyme binding is favored by the coplanar structure and does not require a high concentration of charge on the onium function. The shape of the hydrophobic structures markedly influences binding."}
{"id": "PMID:480158", "title": "Osmolality of parenteral solutions.", "content": "Osmolality-concentration profiles for individual and mixed solute systems are presented. Linear relationships between osmolality and concentration held true in all systems examined at concentrations below 0.2 molal levels. At higher concentrations, linearity existed only in select systems. Deviations from linearity can be greater or less than extrapolated values. In view of the need to determine an osmolarity conversion factor for each parenteral formulation and the many errors possible in the use of these values, adoption of osmolality values for labeling parenteral products rather than osmolarity, as stipulated in USP XIX-NF XIV third supplement, strongly recommended.", "contents": "Osmolality of parenteral solutions. Osmolality-concentration profiles for individual and mixed solute systems are presented. Linear relationships between osmolality and concentration held true in all systems examined at concentrations below 0.2 molal levels. At higher concentrations, linearity existed only in select systems. Deviations from linearity can be greater or less than extrapolated values. In view of the need to determine an osmolarity conversion factor for each parenteral formulation and the many errors possible in the use of these values, adoption of osmolality values for labeling parenteral products rather than osmolarity, as stipulated in USP XIX-NF XIV third supplement, strongly recommended."}
{"id": "PMID:480159", "title": "Preparation of diastereomeric urethane derivatives of atropine and l-hyoscyamine using (-)-1-phenylethylisocyanate.", "content": "Diastereomeric urethane derivatives of atropine (d,l-hyoscyamine) and l-hyoscyamine were prepared by reacting the alkaloids with (-)-1-phenylethylisocyanate. The derivatives, as the picrate ion-pairs, were characterized by their melting points, optical rotations, elemental analyses, and IR, NMR, and UV-visible spectra.", "contents": "Preparation of diastereomeric urethane derivatives of atropine and l-hyoscyamine using (-)-1-phenylethylisocyanate. Diastereomeric urethane derivatives of atropine (d,l-hyoscyamine) and l-hyoscyamine were prepared by reacting the alkaloids with (-)-1-phenylethylisocyanate. The derivatives, as the picrate ion-pairs, were characterized by their melting points, optical rotations, elemental analyses, and IR, NMR, and UV-visible spectra."}
{"id": "PMID:480160", "title": "TLC differentiation of butyrophenone and diphenylbutylpiperidine compounds from phenothiazine derivatives.", "content": "A procedure is described for TLC detection and differentiation of the butyrophenone-diphenylbutylpiperidine group and phenothiazine derivatives at the microgram level. A two-dimensional TLC method to separate butyrophenone and diphenylbutylpiperidine compounds is reported. A variety of possible detection reagents were examined. The solvent systems and spray reagents described should be useful for the identification of these drugs in various dosage forms.", "contents": "TLC differentiation of butyrophenone and diphenylbutylpiperidine compounds from phenothiazine derivatives. A procedure is described for TLC detection and differentiation of the butyrophenone-diphenylbutylpiperidine group and phenothiazine derivatives at the microgram level. A two-dimensional TLC method to separate butyrophenone and diphenylbutylpiperidine compounds is reported. A variety of possible detection reagents were examined. The solvent systems and spray reagents described should be useful for the identification of these drugs in various dosage forms."}
{"id": "PMID:480161", "title": "High-pressure liquid chromatographic analysis of antipyrine in small plasma samples.", "content": "A high-pressure liquid chromatographic (HPLC) method was developed for the assay of antipyrine in small (0.1-ml) plasma samples using aminopyrine as the internal standard and a reversed-phase microparticulate column. The assay sensitivity (1 microgram/ml) permits development of a plasma level--time curve using a single rat. The mean (+/- SE) plasma elmination half-life in rats was 1.28 +/- 0.14 hr. A comparison of the spectrophotometric method with the HPLC method yielded a correlation coefficient of 0.98. The HPLC assay for antipyrine is rapid and precise and can be used for hepatic drug metabolism study in a single animal.", "contents": "High-pressure liquid chromatographic analysis of antipyrine in small plasma samples. A high-pressure liquid chromatographic (HPLC) method was developed for the assay of antipyrine in small (0.1-ml) plasma samples using aminopyrine as the internal standard and a reversed-phase microparticulate column. The assay sensitivity (1 microgram/ml) permits development of a plasma level--time curve using a single rat. The mean (+/- SE) plasma elmination half-life in rats was 1.28 +/- 0.14 hr. A comparison of the spectrophotometric method with the HPLC method yielded a correlation coefficient of 0.98. The HPLC assay for antipyrine is rapid and precise and can be used for hepatic drug metabolism study in a single animal."}
{"id": "PMID:480162", "title": "Metabolic fate of N6-benzyladenosine and N6-benzyladenosine-5'-phosphate in rats.", "content": "The radiolabeled antitumor nucleoside (14C-8)-N6-benzyladenosine and its (14C-8)-5'-phosphate were administered to rats intravenously, and their metabolic fate was studied. Twenty-nine percent of the radioactivity was recovered in the 48-hr urine collection after (14C-8)-N6-benzyladenosine administration. The following metabolites were isolated: unchanged N6-benzyladenosine (20%), adenine (12%), uric acid (5%), and N6-benzyladenine (0.3%). In the case of (14C-8)-N6-benzyladenosine-5'-phosphate, a total of 28% of the radioactivity was recovered in the 48-hr urine collection and the following metabolites were isolated: N6-benzyladenosine (40%), uric acid (12%), adenine (trace), and unidentified urea derivatives (30%). Metabolism of N6-benzyladenosine appears to involve N-debenzylation to some extent, followed by conversion to adenine and uric acid. N6-Benzyladenosine and its 5'-phosphate differ from other adenosine analogs in being retained in significant amounts by the animals.", "contents": "Metabolic fate of N6-benzyladenosine and N6-benzyladenosine-5'-phosphate in rats. The radiolabeled antitumor nucleoside (14C-8)-N6-benzyladenosine and its (14C-8)-5'-phosphate were administered to rats intravenously, and their metabolic fate was studied. Twenty-nine percent of the radioactivity was recovered in the 48-hr urine collection after (14C-8)-N6-benzyladenosine administration. The following metabolites were isolated: unchanged N6-benzyladenosine (20%), adenine (12%), uric acid (5%), and N6-benzyladenine (0.3%). In the case of (14C-8)-N6-benzyladenosine-5'-phosphate, a total of 28% of the radioactivity was recovered in the 48-hr urine collection and the following metabolites were isolated: N6-benzyladenosine (40%), uric acid (12%), adenine (trace), and unidentified urea derivatives (30%). Metabolism of N6-benzyladenosine appears to involve N-debenzylation to some extent, followed by conversion to adenine and uric acid. N6-Benzyladenosine and its 5'-phosphate differ from other adenosine analogs in being retained in significant amounts by the animals."}
{"id": "PMID:480163", "title": "Improvements of dissolution characteristics and chemical stability of 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester by cyclodextrin complexation.", "content": "Inclusion complexation of 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester (I), which is effective in early pregnancy termination, with cyclodextrins in water was ascertained by a solubility study. A solid complex of I-beta-cyclodextrin in a 1:2 molar ratio was obtained, and its dissolution behavior and chemical stability were examined. The results indicated that the complex may have great utility as a rapidly dissolving form of I with prolonged storage time.", "contents": "Improvements of dissolution characteristics and chemical stability of 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester by cyclodextrin complexation. Inclusion complexation of 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester (I), which is effective in early pregnancy termination, with cyclodextrins in water was ascertained by a solubility study. A solid complex of I-beta-cyclodextrin in a 1:2 molar ratio was obtained, and its dissolution behavior and chemical stability were examined. The results indicated that the complex may have great utility as a rapidly dissolving form of I with prolonged storage time."}
{"id": "PMID:480164", "title": "Differential pulse polarography of tetracycline: determination of complexing tendencies of tetracycline analogs in the presence of cations.", "content": "The complexation tendencies, stoichiometries, and stability constants for tetracycline, minocycline, and demeclocycline with the metallic ions calcium(II), magnesium (II), zinc(II), aluminum(III), iron(II), and iron (III) were evaluated using a polarographic technique. Changes in pulse peak heights for each tetracycline deravative were measured as a function of cation concentration. The method provides an in vitro method of evaluating the selectivity of particular metal ions for different tetracycline analogs.", "contents": "Differential pulse polarography of tetracycline: determination of complexing tendencies of tetracycline analogs in the presence of cations. The complexation tendencies, stoichiometries, and stability constants for tetracycline, minocycline, and demeclocycline with the metallic ions calcium(II), magnesium (II), zinc(II), aluminum(III), iron(II), and iron (III) were evaluated using a polarographic technique. Changes in pulse peak heights for each tetracycline deravative were measured as a function of cation concentration. The method provides an in vitro method of evaluating the selectivity of particular metal ions for different tetracycline analogs."}
{"id": "PMID:480165", "title": "Photolytic decomposition of indapamide.", "content": "Photolytic decomposition of indapamide (I) in nitrogen-flushed methanol yields 3-sulfamoyl-4-chlorobenzamide (II), 2-methylindoline (III), semicarbazide (IV), and 1-(N-formamido)-2-methylindoline (V); in oxygen-flushed methanol, II--V, 1-aminocarboxymethyl-2-methylindoline (VI), 3-sulfamoyl-4-chlorobenzoic acid (VII), methyl-3-sulfamoyl-4-chlorobenzoate (VIII), and 2-(N-acetamido)-benzoic acid (IX) are formed. A comparison is made with thermal decomposition of I.", "contents": "Photolytic decomposition of indapamide. Photolytic decomposition of indapamide (I) in nitrogen-flushed methanol yields 3-sulfamoyl-4-chlorobenzamide (II), 2-methylindoline (III), semicarbazide (IV), and 1-(N-formamido)-2-methylindoline (V); in oxygen-flushed methanol, II--V, 1-aminocarboxymethyl-2-methylindoline (VI), 3-sulfamoyl-4-chlorobenzoic acid (VII), methyl-3-sulfamoyl-4-chlorobenzoate (VIII), and 2-(N-acetamido)-benzoic acid (IX) are formed. A comparison is made with thermal decomposition of I."}
{"id": "PMID:480166", "title": "Improved spectrophotometric determination of glycerol and its comparison with an enzymatic method.", "content": "A chemical method for the determination of glycerol was developed and compared to an enzymatic assay for sensitivity and reproducibility. The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as little as 5 micrograms of glycerol/ml can be detected. The enzymatic assay is based on enzymatic glycerol phosphorylation followed by glycerol phosphate dehydrogenation by nadide (nicotinamide adenine dinucleotide). The reduced nadide is used to reduce iodonitrotetrazolium violet to its colored formazan product. The enzymatic method can be used to deterine 50 micrograms of glycerol/ml in aqueous samples.", "contents": "Improved spectrophotometric determination of glycerol and its comparison with an enzymatic method. A chemical method for the determination of glycerol was developed and compared to an enzymatic assay for sensitivity and reproducibility. The chemical assay is based on glycerol oxidation to formaldehyde and subsequent reaction with chromotropic acid to yield a colored product. With this method, as little as 5 micrograms of glycerol/ml can be detected. The enzymatic assay is based on enzymatic glycerol phosphorylation followed by glycerol phosphate dehydrogenation by nadide (nicotinamide adenine dinucleotide). The reduced nadide is used to reduce iodonitrotetrazolium violet to its colored formazan product. The enzymatic method can be used to deterine 50 micrograms of glycerol/ml in aqueous samples."}
{"id": "PMID:480167", "title": "Clearance constants in physiologically based pharmacokinetic models.", "content": "The intrinsic clearance of an organ is usually approximated by the apparent clearance from that organ in the development of a physiologically based pharmacokinetic model. In this study, the exact relationship between the two clearances was derived and analyzed. When the extraction ratio of the drug was small (less than 0.05), the approximation was reasonable. However, when the extraction ratio was high (greater than 0.2), serious errors could be made by using the approximation. These errors could be as much as 50% reduction in the estimated extraction ratio and as much as an order-of-magnitude difference in the intrinsic clearance.", "contents": "Clearance constants in physiologically based pharmacokinetic models. The intrinsic clearance of an organ is usually approximated by the apparent clearance from that organ in the development of a physiologically based pharmacokinetic model. In this study, the exact relationship between the two clearances was derived and analyzed. When the extraction ratio of the drug was small (less than 0.05), the approximation was reasonable. However, when the extraction ratio was high (greater than 0.2), serious errors could be made by using the approximation. These errors could be as much as 50% reduction in the estimated extraction ratio and as much as an order-of-magnitude difference in the intrinsic clearance."}
{"id": "PMID:480171", "title": "In vivo methotrexate transport in murine Lewis lung tumor.", "content": "Methotrexate uptake by murine Lewis lung tumor was measured in vivo over a wide dose range. The data were analyzed according to a model previously developed for tissues in which methotrexate uptake is rate limited by transport across the cell membrane. Methotrexate transport in this tumor followed Michaelis-Menten kinetics with a rate constant for permeability (k/K) of 0.012 min-1. The methotrexate binding capacity of dihydrofolate reductase in the tumor was not exceeded at any dose studied. A low membrane permeability in conjunction with a high dihydrofolate reductase level explains the resistance of this tumor to methotrexate.", "contents": "In vivo methotrexate transport in murine Lewis lung tumor. Methotrexate uptake by murine Lewis lung tumor was measured in vivo over a wide dose range. The data were analyzed according to a model previously developed for tissues in which methotrexate uptake is rate limited by transport across the cell membrane. Methotrexate transport in this tumor followed Michaelis-Menten kinetics with a rate constant for permeability (k/K) of 0.012 min-1. The methotrexate binding capacity of dihydrofolate reductase in the tumor was not exceeded at any dose studied. A low membrane permeability in conjunction with a high dihydrofolate reductase level explains the resistance of this tumor to methotrexate."}
{"id": "PMID:480172", "title": "Effect of enzyme-inducing and enzyme-inhibiting agents on drug absorption. II. Influence in proadifen on 3-O-methylglucose transport in rats.", "content": "Studies utilizing the in vitro everted rat jejunum were performed to investigate the effects of proadifen pretreatment and fasting on active and passive intestinal transfer of 3-O-methylglucose. Animals were pretreated 24 hr prior to the experiments with 100 mg of proadifen/kg ip. With mucosal concentrations of 0.1 mM 3-O-methylglucose and 14C-3-O-methylglucose, transfer to the sugar-free serosal buffer after pretreatment with proadifen was 50% greater than controls after a 24-hr fast and 120% greater than controls after a 48-hr fast. Everted intestinal segments obtained from unfasted control animals showed diminished ability to transfer the sugar derivative, with transfer rates approximately 50% less than those of segments obtained from 24-hr fasted control animals. Wet and dry tissue weights for 24- and 48 hr-fasted groups increased following proadifen pretreatment. The results suggest that proadifen enhances 3-O-methylglucose transport across the rat jejunum and also increases intestinal tissue weight.", "contents": "Effect of enzyme-inducing and enzyme-inhibiting agents on drug absorption. II. Influence in proadifen on 3-O-methylglucose transport in rats. Studies utilizing the in vitro everted rat jejunum were performed to investigate the effects of proadifen pretreatment and fasting on active and passive intestinal transfer of 3-O-methylglucose. Animals were pretreated 24 hr prior to the experiments with 100 mg of proadifen/kg ip. With mucosal concentrations of 0.1 mM 3-O-methylglucose and 14C-3-O-methylglucose, transfer to the sugar-free serosal buffer after pretreatment with proadifen was 50% greater than controls after a 24-hr fast and 120% greater than controls after a 48-hr fast. Everted intestinal segments obtained from unfasted control animals showed diminished ability to transfer the sugar derivative, with transfer rates approximately 50% less than those of segments obtained from 24-hr fasted control animals. Wet and dry tissue weights for 24- and 48 hr-fasted groups increased following proadifen pretreatment. The results suggest that proadifen enhances 3-O-methylglucose transport across the rat jejunum and also increases intestinal tissue weight."}
{"id": "PMID:480173", "title": "Bioavailability and dissolution parameters of seven lithium carbonate products.", "content": "Seven commercial products and a standard powder of lithium carbonate were administered to healthy human volunteers in a crossover study. An analysis of variance of saliva levels and urinary excretion as well as an analysis of variance of peak concentration and the area under the curve from 0 to 24 hr for the saliva levels showed no significant difference between the powder and products, but a significant difference between subjects. A significant difference was found between the time of peak saliva levels, which was attributed to faster powder absorption. A dissolution study using the USP basket method at 50 and 100 rpm and the Levy beaker at 50 rpm also showed no significant difference between products after the lag time for the capsule dosage forms. With a regression analysis, a significant correlation was found between the saliva levels of the products at 2 hr and dissolution in the USP basket at 50 rpm at 4 min.", "contents": "Bioavailability and dissolution parameters of seven lithium carbonate products. Seven commercial products and a standard powder of lithium carbonate were administered to healthy human volunteers in a crossover study. An analysis of variance of saliva levels and urinary excretion as well as an analysis of variance of peak concentration and the area under the curve from 0 to 24 hr for the saliva levels showed no significant difference between the powder and products, but a significant difference between subjects. A significant difference was found between the time of peak saliva levels, which was attributed to faster powder absorption. A dissolution study using the USP basket method at 50 and 100 rpm and the Levy beaker at 50 rpm also showed no significant difference between products after the lag time for the capsule dosage forms. With a regression analysis, a significant correlation was found between the saliva levels of the products at 2 hr and dissolution in the USP basket at 50 rpm at 4 min."}
{"id": "PMID:480174", "title": "Synthesis of substituted 2-aminopyrrole analogs of lidocaine II.", "content": "The synthesis and local anesthetic and antiarrhythmic properties of eight substituted 2-diethylaminoacetamido-3-carbamyl-4-methylpyrroles are described. Three compounds showed significant local anesthetic activity by the guinea pig wheal test, and four showed antiarrhythmic activity against chloroform-induced ventricular arrhythmias in mice.", "contents": "Synthesis of substituted 2-aminopyrrole analogs of lidocaine II. The synthesis and local anesthetic and antiarrhythmic properties of eight substituted 2-diethylaminoacetamido-3-carbamyl-4-methylpyrroles are described. Three compounds showed significant local anesthetic activity by the guinea pig wheal test, and four showed antiarrhythmic activity against chloroform-induced ventricular arrhythmias in mice."}
{"id": "PMID:480175", "title": "Automated analysis of chlorthalidone.", "content": "Chlorthalidone inhibition of the enzymatic hydrolysis rate of p-nitrophenyl acetate by bovine erythrocyte carbonic anhydrase was used as a basis for chlorthalidone determination in plasma and urine. For urinary samples, a completely automated, continuous flow system was developed to extract the samples and perform the enzymatic reaction. Over 100 samples per day could be assayed by one person. The assay had a sensitivity of 0.5 micrograms/ml and thus could determine urinary concentrations after a therapeutic chlorthalidone dose. To determine plasma concentrations after a therapeutic dose, a manual extraction procedure was used in combination with a second continuous flow system for the enzymatic reaction. This system was optimized to detect the lowest chlorthalidone concentration allowed by the enzymatic inhibition constant and could detect 25 ng/ml.", "contents": "Automated analysis of chlorthalidone. Chlorthalidone inhibition of the enzymatic hydrolysis rate of p-nitrophenyl acetate by bovine erythrocyte carbonic anhydrase was used as a basis for chlorthalidone determination in plasma and urine. For urinary samples, a completely automated, continuous flow system was developed to extract the samples and perform the enzymatic reaction. Over 100 samples per day could be assayed by one person. The assay had a sensitivity of 0.5 micrograms/ml and thus could determine urinary concentrations after a therapeutic chlorthalidone dose. To determine plasma concentrations after a therapeutic dose, a manual extraction procedure was used in combination with a second continuous flow system for the enzymatic reaction. This system was optimized to detect the lowest chlorthalidone concentration allowed by the enzymatic inhibition constant and could detect 25 ng/ml."}
{"id": "PMID:480176", "title": "Progestin permeation through polymer membranes IV: Mechanism of steroid permeation and functional group contributions to diffusion through hydrogel films.", "content": "Hydrogel films were prepared from hydroxyethyl methacrylate, both with (Film II) and withouth (Film I) 5.25 mole% of ethylene glycol dimethacrylate. Permeation, diffusion, and partition coefficients for progesterone, testosterone, nandrolone, norethindrone, 17 alpha-hydroxyprogesterone, estradiol, and hydrocortisone were determined. A solute permeation model was proposed based on the separation of a domain (B) composed of \"bulk-like\" water and a doman (A) composed of polymer, interfacial water, and bound water present in the films. The separate contributions from the \"pore\" and \"solution-diffusion\" mechanisms to the total permeability were calculated from the model. Steroid permeabilities through Films I and II were analyzed in accordance with this model. Permeation of Film II occurred via the solution-diffusion mechanism. Permeation of Film I occurred predominately by the pore mechanism with a small contribution (approximate 20%) from the solution-diffusion mechanism. The latter contribution was dependent on the solubility of the solute within the A domains of the hydrogel film. Functional group contributions to permeation of Film II were ascribed to either steric or hydrogen bonding effects.", "contents": "Progestin permeation through polymer membranes IV: Mechanism of steroid permeation and functional group contributions to diffusion through hydrogel films. Hydrogel films were prepared from hydroxyethyl methacrylate, both with (Film II) and withouth (Film I) 5.25 mole% of ethylene glycol dimethacrylate. Permeation, diffusion, and partition coefficients for progesterone, testosterone, nandrolone, norethindrone, 17 alpha-hydroxyprogesterone, estradiol, and hydrocortisone were determined. A solute permeation model was proposed based on the separation of a domain (B) composed of \"bulk-like\" water and a doman (A) composed of polymer, interfacial water, and bound water present in the films. The separate contributions from the \"pore\" and \"solution-diffusion\" mechanisms to the total permeability were calculated from the model. Steroid permeabilities through Films I and II were analyzed in accordance with this model. Permeation of Film II occurred via the solution-diffusion mechanism. Permeation of Film I occurred predominately by the pore mechanism with a small contribution (approximate 20%) from the solution-diffusion mechanism. The latter contribution was dependent on the solubility of the solute within the A domains of the hydrogel film. Functional group contributions to permeation of Film II were ascribed to either steric or hydrogen bonding effects."}
{"id": "PMID:480177", "title": "Simple field test for marijuana.", "content": "A simple, rapid, sensitive, and specific field test for marijuana and its products is described. The test employs an extracting-eluting ssolvent and an inert adsorbent microcolumn coated with Fast Blue B salt as the reagent. One milligram or less of the suspect material can be tested within 1 min. Of greater than nonmarijuana plant samples tested, only nutmeg and its mace responded similarly to marijuana initially. A differentiation was possible by careful evaluation of the colored microcolumn and the elution behavior of the colored products. The test also provides for a confirmatory step, based on spectrophotometric analysis of the colored eluates. The reagent microcolumn was very stable to environmental factors such as moisture, light, and air.", "contents": "Simple field test for marijuana. A simple, rapid, sensitive, and specific field test for marijuana and its products is described. The test employs an extracting-eluting ssolvent and an inert adsorbent microcolumn coated with Fast Blue B salt as the reagent. One milligram or less of the suspect material can be tested within 1 min. Of greater than nonmarijuana plant samples tested, only nutmeg and its mace responded similarly to marijuana initially. A differentiation was possible by careful evaluation of the colored microcolumn and the elution behavior of the colored products. The test also provides for a confirmatory step, based on spectrophotometric analysis of the colored eluates. The reagent microcolumn was very stable to environmental factors such as moisture, light, and air."}
{"id": "PMID:480178", "title": "Metabolic performance and GI function in magnesium-deficient rats.", "content": "A quantitative evaluation of the mass balance and GI motor effects of dietary magnesium deficiency in the adult male Sprague-Dawley rat is described. Seventy-seven animals were used. Both sham control and experimental groups were maintained on a commercial stock laboratory diet ad libitum for 30 days, after which the experimental rats were switched for 30 days to magnesium-deficient diet ad libitum, containing a magnesium concentration of 16.2 ppm. Ten rats were used to determine the acetylcholine responsiveness of duodenal muscle segments in vitro. In all cases, the segments from the deficient rats were hyperresponsive to a fixed acetylcholine dose. Forty-six rats were used to determine the average intestinal transit rate, which increased significantly in 30-day magnesium-deficient rats. A final series employed 21 rats who were housed in individual metabolic cages. After 5 days on the deficient diet, the average daily fecal pellet counts and fecal weights were significantly reduced. It is concluded that chronic magnesium deficiency is associated with altered GI motor function in the adult male rat.", "contents": "Metabolic performance and GI function in magnesium-deficient rats. A quantitative evaluation of the mass balance and GI motor effects of dietary magnesium deficiency in the adult male Sprague-Dawley rat is described. Seventy-seven animals were used. Both sham control and experimental groups were maintained on a commercial stock laboratory diet ad libitum for 30 days, after which the experimental rats were switched for 30 days to magnesium-deficient diet ad libitum, containing a magnesium concentration of 16.2 ppm. Ten rats were used to determine the acetylcholine responsiveness of duodenal muscle segments in vitro. In all cases, the segments from the deficient rats were hyperresponsive to a fixed acetylcholine dose. Forty-six rats were used to determine the average intestinal transit rate, which increased significantly in 30-day magnesium-deficient rats. A final series employed 21 rats who were housed in individual metabolic cages. After 5 days on the deficient diet, the average daily fecal pellet counts and fecal weights were significantly reduced. It is concluded that chronic magnesium deficiency is associated with altered GI motor function in the adult male rat."}
{"id": "PMID:480179", "title": "Absorption and penetration of dinoprost (prostaglandin F2 alpha) and dinoprost methyl ester into perfused mesenteric circulation in rats.", "content": "The absorption of dinoprost (prostaglandin F2 alpha) and its methyl ester in rat jejunum was studied. A 22-cm segment of rat jejunum was cannulated at both ends and connected to an oscillating perfusion pump system. The mesenteric vasculature supplying this isolated segment also was cannulated and perfused with Kreb's bicarbonate buffer with dextran. Solutions of 3H-dinoprost or its methyl ester were introduced into the lumen and oscillated through the segment. The disappearance of radioactivity from the lumen and the appearance of radioactivity in the vascular perfusate were monitored. The metabolite patterns in the vascular perfusate were analyzed by TLC. A lag between the time the drug disappeared from the lumen and the time it appeared in the mesenteric circulation was detected. This lag was longer for the methyl ester than for the free acid, even though the ester disappears from the lumen faster than does the free acid. Upon removal of dinoprost from the gut lumen, a gradual decrease in the amount of drug appearing in the mesenteric circulation could be detected. However, with the ester, a slight increase could be observed for approximately 0.5 hr, followed by a decrease. Metabolism by the gut wall appears to be greater for the ester than for the acid. The results suggest that, although the ester disappears from the lumen more quickly than does the acid, it actually penetrates to the blood at a slower rate and undergoes greater metabolism.", "contents": "Absorption and penetration of dinoprost (prostaglandin F2 alpha) and dinoprost methyl ester into perfused mesenteric circulation in rats. The absorption of dinoprost (prostaglandin F2 alpha) and its methyl ester in rat jejunum was studied. A 22-cm segment of rat jejunum was cannulated at both ends and connected to an oscillating perfusion pump system. The mesenteric vasculature supplying this isolated segment also was cannulated and perfused with Kreb's bicarbonate buffer with dextran. Solutions of 3H-dinoprost or its methyl ester were introduced into the lumen and oscillated through the segment. The disappearance of radioactivity from the lumen and the appearance of radioactivity in the vascular perfusate were monitored. The metabolite patterns in the vascular perfusate were analyzed by TLC. A lag between the time the drug disappeared from the lumen and the time it appeared in the mesenteric circulation was detected. This lag was longer for the methyl ester than for the free acid, even though the ester disappears from the lumen faster than does the free acid. Upon removal of dinoprost from the gut lumen, a gradual decrease in the amount of drug appearing in the mesenteric circulation could be detected. However, with the ester, a slight increase could be observed for approximately 0.5 hr, followed by a decrease. Metabolism by the gut wall appears to be greater for the ester than for the acid. The results suggest that, although the ester disappears from the lumen more quickly than does the acid, it actually penetrates to the blood at a slower rate and undergoes greater metabolism."}
{"id": "PMID:480180", "title": "Effects of aspirin on 14C-pirprofen disposition in rats.", "content": "The effects of aspirin on 14C-pirprofen disposition in the rat were studied. An oral 60-mg/kg dose of aspirin significantly reduced plasma radioactivity during the 1--8-hr interval after an intravenous 5-mg/kg injection of 14C-pirprofen. The aspirin-treated group had only 69% as much area under the radioactivity curve as the control group. The radioactive material in plasma consisted almost entirely of 14C-pirprofen, as shown by GLC. The plasma clearance of 14C-pirprofen was 7.4 ml/hr for the aspirin-treated group and 5.1 ml/hr for the control group, while the volumes of distribution were 0.32 and 0.20 liter/kg, respectively. The apparent elimination half-life was unchanged at 5.9 hr. 14C-Pirprofen was approximately 98.6% bound to plasma proteins, and the binding decreased to an average of 97.2% in the presence of salicylate. Binding to blood cellular constituents was insignificant. Rats give 14C-pirprofen by intravenous injection without aspirin secreted 36.0--42.8% of the dose radioactivity into bile during 4 hr while a comparable group given 60 mg of aspirin/kg secreted 46.4--70.8%. TLC and GLC demonstrated that the radioactivity in rat bile was 80--90% conjugated 14C-pirprofen. The increased radioactive material secretion into bile was compensated in the intact rat by reabsorption, since the total radioactive material excreted in urine was not changed by aspirin administration.", "contents": "Effects of aspirin on 14C-pirprofen disposition in rats. The effects of aspirin on 14C-pirprofen disposition in the rat were studied. An oral 60-mg/kg dose of aspirin significantly reduced plasma radioactivity during the 1--8-hr interval after an intravenous 5-mg/kg injection of 14C-pirprofen. The aspirin-treated group had only 69% as much area under the radioactivity curve as the control group. The radioactive material in plasma consisted almost entirely of 14C-pirprofen, as shown by GLC. The plasma clearance of 14C-pirprofen was 7.4 ml/hr for the aspirin-treated group and 5.1 ml/hr for the control group, while the volumes of distribution were 0.32 and 0.20 liter/kg, respectively. The apparent elimination half-life was unchanged at 5.9 hr. 14C-Pirprofen was approximately 98.6% bound to plasma proteins, and the binding decreased to an average of 97.2% in the presence of salicylate. Binding to blood cellular constituents was insignificant. Rats give 14C-pirprofen by intravenous injection without aspirin secreted 36.0--42.8% of the dose radioactivity into bile during 4 hr while a comparable group given 60 mg of aspirin/kg secreted 46.4--70.8%. TLC and GLC demonstrated that the radioactivity in rat bile was 80--90% conjugated 14C-pirprofen. The increased radioactive material secretion into bile was compensated in the intact rat by reabsorption, since the total radioactive material excreted in urine was not changed by aspirin administration."}
{"id": "PMID:480181", "title": "Doxycycline determination in human serum and urine by high-performance liquid chromatography.", "content": "A reversed-phase high-performance liquid chromatographic method for quantitative doxycycline determination in human serum and urine is described. The drug was extracted from buffered (pH 6.1) serum or urine into ethyl acetate. A structural analog, demeclocycline, was added as the internal standard. A 10-cm X 2-mm i.d., 5-micrometers Lichrosorb RP8 column with acetonitrile-0.1 M citric acid as the eluent was used. The effluent was monitored at 350 nm. The extraction recovery from spiked serum was 87-8 +/- 4.3% (mean +/- SD, n = 11); for urine, a value of 92.2 +/-2.0% (mean +/- SD, n = 10) was found. Within-run and within-day relative standard deviations averaged (x = 2.5 micrograms/ml, n = 10) and 4.75% (x = 2.6 micrograms/ml, n = 9), respectively. The detection limit was estimated at 50 ng/ml of serum. No significant extra peaks were observed in chromatograms obtained on serum or urine extracts, suggesting the probable absence of metabolic processes in vivo.", "contents": "Doxycycline determination in human serum and urine by high-performance liquid chromatography. A reversed-phase high-performance liquid chromatographic method for quantitative doxycycline determination in human serum and urine is described. The drug was extracted from buffered (pH 6.1) serum or urine into ethyl acetate. A structural analog, demeclocycline, was added as the internal standard. A 10-cm X 2-mm i.d., 5-micrometers Lichrosorb RP8 column with acetonitrile-0.1 M citric acid as the eluent was used. The effluent was monitored at 350 nm. The extraction recovery from spiked serum was 87-8 +/- 4.3% (mean +/- SD, n = 11); for urine, a value of 92.2 +/-2.0% (mean +/- SD, n = 10) was found. Within-run and within-day relative standard deviations averaged (x = 2.5 micrograms/ml, n = 10) and 4.75% (x = 2.6 micrograms/ml, n = 9), respectively. The detection limit was estimated at 50 ng/ml of serum. No significant extra peaks were observed in chromatograms obtained on serum or urine extracts, suggesting the probable absence of metabolic processes in vivo."}
{"id": "PMID:480182", "title": "Microiontophoretic studies on noradrenergic inhibition from locus coeruleus of spinal trigeminal nucleus neurons.", "content": "Microiontophoretic studies using cats anesthetized with alpha-chloralose and local anaesthetic were undertaken in an attempt to determine whether or not norepinephrine (NE) derived from the locus coeruleus (LC) acts on neurons of the spinal trigeminal nucleus (STN) as an inhibitory transmitter. Both conditioning stimulation of the LC and iontophoretic application of NE inhibited orthodromic spike generation by interior alveolar nerve stimulation in 9 out of 14 STN relay neurons, without affecting antidromic spikes elicited by stimulation of the contralateral medial lemniscus. Glutamate-induced spike firing of the STN relay neurons, however, remained unaltered with iontophoretic application of NE up to 200 nA. The LC conditioning stimulation inhibited orthodromic spikes in 22 out of 25 type-B interneurons in the STN, while iontophoretic NE application seldom had an effect on orthodromic spikes and spontaneous firing of the interneurons. Only 5 out of the 25 interneurons exhibited an inhibition of orthodromic spike generation and spontaneous firing with iontophoretic NE. These neurons fired spikes upon orthodromic stimulation with a relatively short latency, compared with the spike latency of neurons unaffected by iontophoretic NE. The present results strongly suggest that NE released from the terminals of the LC neurons inhibits transmission of STN relay neurons, probably by acting on primary afferent terminals to produce presynaptic inhibition.", "contents": "Microiontophoretic studies on noradrenergic inhibition from locus coeruleus of spinal trigeminal nucleus neurons. Microiontophoretic studies using cats anesthetized with alpha-chloralose and local anaesthetic were undertaken in an attempt to determine whether or not norepinephrine (NE) derived from the locus coeruleus (LC) acts on neurons of the spinal trigeminal nucleus (STN) as an inhibitory transmitter. Both conditioning stimulation of the LC and iontophoretic application of NE inhibited orthodromic spike generation by interior alveolar nerve stimulation in 9 out of 14 STN relay neurons, without affecting antidromic spikes elicited by stimulation of the contralateral medial lemniscus. Glutamate-induced spike firing of the STN relay neurons, however, remained unaltered with iontophoretic application of NE up to 200 nA. The LC conditioning stimulation inhibited orthodromic spikes in 22 out of 25 type-B interneurons in the STN, while iontophoretic NE application seldom had an effect on orthodromic spikes and spontaneous firing of the interneurons. Only 5 out of the 25 interneurons exhibited an inhibition of orthodromic spike generation and spontaneous firing with iontophoretic NE. These neurons fired spikes upon orthodromic stimulation with a relatively short latency, compared with the spike latency of neurons unaffected by iontophoretic NE. The present results strongly suggest that NE released from the terminals of the LC neurons inhibits transmission of STN relay neurons, probably by acting on primary afferent terminals to produce presynaptic inhibition."}
{"id": "PMID:480183", "title": "Clofibrate displaces warfarin from plasma proteins in man: an example of a pure displacement interaction.", "content": "Clofibrate is known to potentiate the anticoagulant effect of warfarin during chronic administration. We examined the disposition of racemic warfarin in four healthy volunteers before and during clofibrate coadministration using an intravenous-continuous oral sequence of warfarin administration. An interaction between warfarin and clofibrate, evidenced by longer prothrombin and prothrombin-proconvertin times, was seen in all four subjects. Clofibrate caused a displacement of warfarin from plasma protein binding sites, with a 13% increase in the free drug fraction in plasma. As predicted from theoretical considerations, this displacement resulted in a small (18%) increase in the steady-state volume of distribution, and an increase in total plasma clearance, which, for warfarin, is independent on the free fraction of drug in plasma. The net effect of these changes is that the free concentration of warfarin was not changed during clofibrate coadministration, although total plasma concentrations were lower. This study documents the occurrence in man of a displacement pharmacokinetic interaction between clofibrate and warfarin. However, this pharmacokinetic interaction does not account for the clinical interaction between the two drugs, since free warfarin concentrations are unchanged.", "contents": "Clofibrate displaces warfarin from plasma proteins in man: an example of a pure displacement interaction. Clofibrate is known to potentiate the anticoagulant effect of warfarin during chronic administration. We examined the disposition of racemic warfarin in four healthy volunteers before and during clofibrate coadministration using an intravenous-continuous oral sequence of warfarin administration. An interaction between warfarin and clofibrate, evidenced by longer prothrombin and prothrombin-proconvertin times, was seen in all four subjects. Clofibrate caused a displacement of warfarin from plasma protein binding sites, with a 13% increase in the free drug fraction in plasma. As predicted from theoretical considerations, this displacement resulted in a small (18%) increase in the steady-state volume of distribution, and an increase in total plasma clearance, which, for warfarin, is independent on the free fraction of drug in plasma. The net effect of these changes is that the free concentration of warfarin was not changed during clofibrate coadministration, although total plasma concentrations were lower. This study documents the occurrence in man of a displacement pharmacokinetic interaction between clofibrate and warfarin. However, this pharmacokinetic interaction does not account for the clinical interaction between the two drugs, since free warfarin concentrations are unchanged."}
{"id": "PMID:480184", "title": "Effects of clofibrate and warfarin alone and in combination on the disposition of vitamin K1.", "content": "Clofibrate may interact with warfarin by potentiating its effects on vitamin K disposition. To examine this possibility, specifically labeled [3H]vitamin K was given intravenously to four healthy volunteers under conditions of no drug administration, administration of warfarin or clofibrate alone, or co-administration of both drugs. Clofibrate alone did not affect the disposition of tritiated vitamin K. Warfarin alone produced an accumulation in plasma of substantial amounts of vitamin K epoxide, a metabolite of vitamin K which is reconverted to vitamin K by a specific reductase. Although reconversion is apparently blocked to a large extent by warfarin, the plasma disappearance of tritiated vitamin K in the presence of warfarin is almost superimpossible to that observed in the absence of drugs. Clofibrate coadministration did not result in greater accumulation of vitamin K epoxide in plasma. These results indicate that clofibrate does not enhance the inhibition of the reductase enzyme. Analysis of the tritiated vitamin K plasma disappearance data indicates that the pool size of vitamin K in the body is small, and is turned over almost 10 times daily. The vitamin K epoxide data suggest that, in the absence of drugs, a relatively small proportion of the epoxide is reconverted to the vitamin.", "contents": "Effects of clofibrate and warfarin alone and in combination on the disposition of vitamin K1. Clofibrate may interact with warfarin by potentiating its effects on vitamin K disposition. To examine this possibility, specifically labeled [3H]vitamin K was given intravenously to four healthy volunteers under conditions of no drug administration, administration of warfarin or clofibrate alone, or co-administration of both drugs. Clofibrate alone did not affect the disposition of tritiated vitamin K. Warfarin alone produced an accumulation in plasma of substantial amounts of vitamin K epoxide, a metabolite of vitamin K which is reconverted to vitamin K by a specific reductase. Although reconversion is apparently blocked to a large extent by warfarin, the plasma disappearance of tritiated vitamin K in the presence of warfarin is almost superimpossible to that observed in the absence of drugs. Clofibrate coadministration did not result in greater accumulation of vitamin K epoxide in plasma. These results indicate that clofibrate does not enhance the inhibition of the reductase enzyme. Analysis of the tritiated vitamin K plasma disappearance data indicates that the pool size of vitamin K in the body is small, and is turned over almost 10 times daily. The vitamin K epoxide data suggest that, in the absence of drugs, a relatively small proportion of the epoxide is reconverted to the vitamin."}
{"id": "PMID:480185", "title": "Dissociation between opiate-like and antidiarrheal activities of antidiarrheal drugs.", "content": "Three synthetic antidiarrheals, diphenoxylate, loperamide and SC 27166, and two narcotics, morphine and codeine, were evaluated in rats by the intravenous and oral route for specificity and duration of their antidiarrheal, opiate-like and acute toxic effects. The activity in the castor oil test, the tail withdrawal test and the acute toxicity test was used to determine the relative antidiarrheal specificity and relative safety margins. An analysis of animal and clinical data indicate these tests to be excellent indicators of clinical usefulness and specificity. Intravenously, all five agents induced opiate-like central effects, loperamide and SC 27166 at near toxic doses only. When administered orally loperamide and SC 27166 were devoid of opiate-like central nervous system activity. Analysis of the plasma levels after oral loperamide indicated that this drug does not attain a concentration high enough to induce opiate-like central effects. All agents were effective antidiarrheals by the oral route with loperamide being the most potent (ED50 = 0.15 mg/kg), longest acting (ED50 8 hr = 1.81 mg/kg) and most specific (relative antidiarrheal specificity, 8 hr greater than or equal to 88) and having the greatest relative safety margin (8 hr = 102).", "contents": "Dissociation between opiate-like and antidiarrheal activities of antidiarrheal drugs. Three synthetic antidiarrheals, diphenoxylate, loperamide and SC 27166, and two narcotics, morphine and codeine, were evaluated in rats by the intravenous and oral route for specificity and duration of their antidiarrheal, opiate-like and acute toxic effects. The activity in the castor oil test, the tail withdrawal test and the acute toxicity test was used to determine the relative antidiarrheal specificity and relative safety margins. An analysis of animal and clinical data indicate these tests to be excellent indicators of clinical usefulness and specificity. Intravenously, all five agents induced opiate-like central effects, loperamide and SC 27166 at near toxic doses only. When administered orally loperamide and SC 27166 were devoid of opiate-like central nervous system activity. Analysis of the plasma levels after oral loperamide indicated that this drug does not attain a concentration high enough to induce opiate-like central effects. All agents were effective antidiarrheals by the oral route with loperamide being the most potent (ED50 = 0.15 mg/kg), longest acting (ED50 8 hr = 1.81 mg/kg) and most specific (relative antidiarrheal specificity, 8 hr greater than or equal to 88) and having the greatest relative safety margin (8 hr = 102)."}
{"id": "PMID:480187", "title": "Role of peripheral adrenoreceptors and vasopressin in the suppression of plasma renin activity by L-dopa in carbidopa-treated dogs.", "content": "When extracerebral dopa decarboxylase is inhibited by carbidopa, L-dopa lowers plasma renin activity (PRA). The present study was designed to determine whether this suppression of PRA is mediated by the sympathetic nerves, and to identify the peripheral adrenergic receptor types involved. All experiments were performed in pentobarbital-anesthetized dogs in which changes in renal perfusion pressure were minimized by means of a suprarenal aortic clamp. Neither alpha adrenoreceptor blockage with phenoxybenzamine nor beta adrenoreceptor blockade with propranolol was by itself sufficient to block the suppression of PRA by L-dopa with carbidopa. However, combined alpha and beta adrenoreceptor blockade lowered PRA and completely prevented any further suppression of PRA by L-dopa with carbidopa. It was also observed that phenoxybenzamine decreased PRA by 48% when administered to propranolol-treated animals. Taken together, these data indicate that L-dopa with carbidopa suppresses PRA by decreasing sympathetic nerve stimulation of both alpha and beta adrenoreceptors. Plasma vasopressin concnetration was significantly decreased by L-dopa with carbidopa both in the control group and in animals with combined alpha and beta adrenoreceptor blockade. Because plasma vasopressin levels decreased after L-dopa, vasopressin is unlikely to play a causative role in the suppression of PRA.", "contents": "Role of peripheral adrenoreceptors and vasopressin in the suppression of plasma renin activity by L-dopa in carbidopa-treated dogs. When extracerebral dopa decarboxylase is inhibited by carbidopa, L-dopa lowers plasma renin activity (PRA). The present study was designed to determine whether this suppression of PRA is mediated by the sympathetic nerves, and to identify the peripheral adrenergic receptor types involved. All experiments were performed in pentobarbital-anesthetized dogs in which changes in renal perfusion pressure were minimized by means of a suprarenal aortic clamp. Neither alpha adrenoreceptor blockage with phenoxybenzamine nor beta adrenoreceptor blockade with propranolol was by itself sufficient to block the suppression of PRA by L-dopa with carbidopa. However, combined alpha and beta adrenoreceptor blockade lowered PRA and completely prevented any further suppression of PRA by L-dopa with carbidopa. It was also observed that phenoxybenzamine decreased PRA by 48% when administered to propranolol-treated animals. Taken together, these data indicate that L-dopa with carbidopa suppresses PRA by decreasing sympathetic nerve stimulation of both alpha and beta adrenoreceptors. Plasma vasopressin concnetration was significantly decreased by L-dopa with carbidopa both in the control group and in animals with combined alpha and beta adrenoreceptor blockade. Because plasma vasopressin levels decreased after L-dopa, vasopressin is unlikely to play a causative role in the suppression of PRA."}
{"id": "PMID:480190", "title": "Warfarin enantiomer disposition: determination by stereoselective radioimmunoassay.", "content": "Immunoassay techniques offer promise for the selective analysis of enantiomeric substances without prior separation. In a demonstrationof this selective analysis, radioimmunoassays were developed for R- and S-warfarin. The 4'-carboxyethyl analog of R,S-warfarin was resolved and the enantiomeric acids were coupled to bovine serum albumin. Immunization of rabbits with the conjugates led to formation of antisera which selectively bound the predicted enantiomer. Cross-reactions with various metabolites of warfarin were low (0.1--4%) except for 4'-hydroxywarfarin, as were cross-reactions with the opposite enantiomer (0.3--3%). Assays were developed and used to determine enantiomer half-lives in rats dosed with racemic warfarin. Results were consistent with those found previously by administration of individual enantiomers, thus indicating the utility of the radioimmunoassay method.", "contents": "Warfarin enantiomer disposition: determination by stereoselective radioimmunoassay. Immunoassay techniques offer promise for the selective analysis of enantiomeric substances without prior separation. In a demonstrationof this selective analysis, radioimmunoassays were developed for R- and S-warfarin. The 4'-carboxyethyl analog of R,S-warfarin was resolved and the enantiomeric acids were coupled to bovine serum albumin. Immunization of rabbits with the conjugates led to formation of antisera which selectively bound the predicted enantiomer. Cross-reactions with various metabolites of warfarin were low (0.1--4%) except for 4'-hydroxywarfarin, as were cross-reactions with the opposite enantiomer (0.3--3%). Assays were developed and used to determine enantiomer half-lives in rats dosed with racemic warfarin. Results were consistent with those found previously by administration of individual enantiomers, thus indicating the utility of the radioimmunoassay method."}
{"id": "PMID:480193", "title": "Investigation of the drug-binding defect in plasma from rats with glycerol-induced acute renal failure.", "content": "The binding defect in plasma from rats with glycerol-induced acute renal failure has been studied. The protein binding of phenytoin, salicylic acid, o-methyl red and methyl orange in uremic rat plasma, measured by equilibrium dialysis at 37 degrees C, was significantly decreased. Analysis of the data by a modified Scatchard plot revealed that in uremic plasma the total binding capacity (nkP) was decreased mainly as the result of a decrease in apparent association constant (k). Charcoal extraction significantly increased the ability of uremic rat plasma to bind the four ligands and restored the binding to about that of the control plasma. Extraction with organic solvents or silicic acid and pre-dialysis against defatted human albumin produced no significant removal of the binding defect. The thiol concentrations of male rat plasma and female rat serum were significantly decreased in uremia. Dithiothreitol decreased the binding of o-methyl red to both control and uremic rat plasma and thereby largely removed the pretreatment difference in unbound fraction of dye. The results suggest the presence of endogenous binding inhibitors.", "contents": "Investigation of the drug-binding defect in plasma from rats with glycerol-induced acute renal failure. The binding defect in plasma from rats with glycerol-induced acute renal failure has been studied. The protein binding of phenytoin, salicylic acid, o-methyl red and methyl orange in uremic rat plasma, measured by equilibrium dialysis at 37 degrees C, was significantly decreased. Analysis of the data by a modified Scatchard plot revealed that in uremic plasma the total binding capacity (nkP) was decreased mainly as the result of a decrease in apparent association constant (k). Charcoal extraction significantly increased the ability of uremic rat plasma to bind the four ligands and restored the binding to about that of the control plasma. Extraction with organic solvents or silicic acid and pre-dialysis against defatted human albumin produced no significant removal of the binding defect. The thiol concentrations of male rat plasma and female rat serum were significantly decreased in uremia. Dithiothreitol decreased the binding of o-methyl red to both control and uremic rat plasma and thereby largely removed the pretreatment difference in unbound fraction of dye. The results suggest the presence of endogenous binding inhibitors."}
{"id": "PMID:480194", "title": "The influence of endogenous glucagon release on hyperglycemic responses to catecholamines in normal fed and diabetic rats.", "content": "The present study provides evidence that changes in glucagon secretion are influential in determining the hyperglycemic activity of catecholamines in normal and diabetic rats. In normal fed rats, epinephrine (EPI) stimulated large increments in glucagon release and inhibited insulin secretion. In contrast, the modest hyperglycemic activity of isoproterenol (ISO) in normal fed rats correlated with its weak glucagon-releasing activity and its strong insulin-releasing activity. In alloxan-diabetic rats, the augmented hyperglycemic response to ISO was accompanied by larger increments in plasma glucagon levels than the catecholamine produced in normal fed rats. When glucagon release was inhibited by a concurrent infusion of somatostatin, the hyperglycemic responses of normal fed rats to EPI were reduced by approximately 67%. ISO-induced hyperglycemia in alloxan-diabetic rats was even more sensitive to inhibition by somatostatin since this response was reduced by approximately 90% when glucagon release was inhibited by somatostatin. These findings indicate that more than half of the hyperglycemic response to EPI in normal fed rats and nearly all of the hyperglycemia produced by ISO in diabetic rats result from increased glucagon release. Moreover, the impotence of ISO as a hyperglycemic agent in normal fed rats is probably due to insulin release which tends to suppress glucagon release.", "contents": "The influence of endogenous glucagon release on hyperglycemic responses to catecholamines in normal fed and diabetic rats. The present study provides evidence that changes in glucagon secretion are influential in determining the hyperglycemic activity of catecholamines in normal and diabetic rats. In normal fed rats, epinephrine (EPI) stimulated large increments in glucagon release and inhibited insulin secretion. In contrast, the modest hyperglycemic activity of isoproterenol (ISO) in normal fed rats correlated with its weak glucagon-releasing activity and its strong insulin-releasing activity. In alloxan-diabetic rats, the augmented hyperglycemic response to ISO was accompanied by larger increments in plasma glucagon levels than the catecholamine produced in normal fed rats. When glucagon release was inhibited by a concurrent infusion of somatostatin, the hyperglycemic responses of normal fed rats to EPI were reduced by approximately 67%. ISO-induced hyperglycemia in alloxan-diabetic rats was even more sensitive to inhibition by somatostatin since this response was reduced by approximately 90% when glucagon release was inhibited by somatostatin. These findings indicate that more than half of the hyperglycemic response to EPI in normal fed rats and nearly all of the hyperglycemia produced by ISO in diabetic rats result from increased glucagon release. Moreover, the impotence of ISO as a hyperglycemic agent in normal fed rats is probably due to insulin release which tends to suppress glucagon release."}
{"id": "PMID:480198", "title": "Size, scatter and coverage of ganglion cell receptive field centres in the cat retina.", "content": "1. Receptive field centre sizes of brisk-sustained (X) and brisk-transient (Y) ganglion cells of the cat retina were assessed by three different methods: small spot mapping, area threshold method and spatial resolution. 2. Centre sizes of brisk-sustained (X) cells increased from 20' in the central area to about 70' at an eccentricity of 4.5 mm, centre sizes of brisk-transient (Y) cells from 50' in the central area to about 140' at 5 mm eccentricity. 3. The scatter of centre sizes at one retinal location was measured by recording as many ganglion cells as possible in one cat in a small field of retina. The centre sizes of the individual classes were homogeneous and exhibited only a small amount of scatter. 4. The coverage of the retina by the different ganglion cell classes was assessed from their density and their receptive field centre area. At every retinal location the receptive field centres of seven to twenty brisk-sustained (X) cells and of three to six brisk-transient (Y) cells were found to overlap. Sluggish concentric and non-concentric cells taken together have a coverage factor of about 60.", "contents": "Size, scatter and coverage of ganglion cell receptive field centres in the cat retina. 1. Receptive field centre sizes of brisk-sustained (X) and brisk-transient (Y) ganglion cells of the cat retina were assessed by three different methods: small spot mapping, area threshold method and spatial resolution. 2. Centre sizes of brisk-sustained (X) cells increased from 20' in the central area to about 70' at an eccentricity of 4.5 mm, centre sizes of brisk-transient (Y) cells from 50' in the central area to about 140' at 5 mm eccentricity. 3. The scatter of centre sizes at one retinal location was measured by recording as many ganglion cells as possible in one cat in a small field of retina. The centre sizes of the individual classes were homogeneous and exhibited only a small amount of scatter. 4. The coverage of the retina by the different ganglion cell classes was assessed from their density and their receptive field centre area. At every retinal location the receptive field centres of seven to twenty brisk-sustained (X) cells and of three to six brisk-transient (Y) cells were found to overlap. Sluggish concentric and non-concentric cells taken together have a coverage factor of about 60."}
{"id": "PMID:480203", "title": "Dependence of motoneurone properties on the length of immobilized muscle.", "content": "1. The soleus muscle of adult cats was fixed in a shortened or lengthened position for 2 weeks by immobilizing the leg with a plaster cast. To eliminate the effects of reflex activity arising from the muscle, the lumbosacral dorsal roots were sectioned on the side of leg immobilization.2. The soleus muscle showed a significant decrease in weight after immobilization in a shortened position. Immobilization of the muscle in a lengthened position produced no significant changes in its weight.3. Two weeks after transection of the throacic spinal cord, the soleus muscle showed a significant decrease in weight. This decrease was prevented by immobilization of the muscle in a lengthened position. Immobilization in a shortened position caused no further muscle atrophy in cord-transected animals.4. Soleus motoneurones showed a significant decrease in the duration of after-hyperpolarization (a.h.p.) 2 weeks after immobilization of the muscle in a shortened position, whereas immobilization of the muscle in a lengthened position caused no significant changes in the duration of a.h.p.5. A decrease in the duration of a.h.p. of soleus motoneurones induced by cord transection was significantly prevented by immobilization of the muscle in a lengthened position but not by immobilization in a shortened position.6. In a variety of experimental conditions, the mean duration of a.h.p. of soleus motoneurones was significantly correlated with the soleus muscle: body wt. ratio.7. It is concluded that certain motoneurone properties depend upon the condition of the innervated muscle and that the primary factor responsible for the trophic influence is metabolic change in the muscle rather than contractile activity itself.", "contents": "Dependence of motoneurone properties on the length of immobilized muscle. 1. The soleus muscle of adult cats was fixed in a shortened or lengthened position for 2 weeks by immobilizing the leg with a plaster cast. To eliminate the effects of reflex activity arising from the muscle, the lumbosacral dorsal roots were sectioned on the side of leg immobilization.2. The soleus muscle showed a significant decrease in weight after immobilization in a shortened position. Immobilization of the muscle in a lengthened position produced no significant changes in its weight.3. Two weeks after transection of the throacic spinal cord, the soleus muscle showed a significant decrease in weight. This decrease was prevented by immobilization of the muscle in a lengthened position. Immobilization in a shortened position caused no further muscle atrophy in cord-transected animals.4. Soleus motoneurones showed a significant decrease in the duration of after-hyperpolarization (a.h.p.) 2 weeks after immobilization of the muscle in a shortened position, whereas immobilization of the muscle in a lengthened position caused no significant changes in the duration of a.h.p.5. A decrease in the duration of a.h.p. of soleus motoneurones induced by cord transection was significantly prevented by immobilization of the muscle in a lengthened position but not by immobilization in a shortened position.6. In a variety of experimental conditions, the mean duration of a.h.p. of soleus motoneurones was significantly correlated with the soleus muscle: body wt. ratio.7. It is concluded that certain motoneurone properties depend upon the condition of the innervated muscle and that the primary factor responsible for the trophic influence is metabolic change in the muscle rather than contractile activity itself."}
{"id": "PMID:480204", "title": "The excitation and depression of spinal neurones by ibotenic acid.", "content": "1. The firing of spinal interneurones and Renshaw cells by microelectrophoretic (+/-)-ibotenate, which was approximately eight times more active as an excitant than L-glutamate, was followed by prolonged depression of the sensitivity of the neurones to excitant amino acids and acetylcholine. 2. The depression, which lasted for 15--30 min when ibotenate was ejected for 3--6 min, was blocked by the GABA-antagonist bicuculline methochloride, and was independent of prior firing since it occurred with subthreshold concentrations of ibotenate and when ibotenate firing had been blocked by DL-alpha-aminoadipate. 3. When administered electrophoretically for 5 min, muscimol, a potent GABA  agonist, reduced neuronal excitability for prolonged periods and this effect was also prevented by bicuculline methochloride. 4. The depression of neuronal excitability produced by GABA, taurine, isoguvacine or 3-aminopropane sulphonate, ejected for periods of 5--6 min, recovered rapidly. 5. It is suggested that ibotenate is converted in vivo to muscimol or a related compound which has a prolonged, bicuculline-sensitive depressant action on the excitability of neurones.", "contents": "The excitation and depression of spinal neurones by ibotenic acid. 1. The firing of spinal interneurones and Renshaw cells by microelectrophoretic (+/-)-ibotenate, which was approximately eight times more active as an excitant than L-glutamate, was followed by prolonged depression of the sensitivity of the neurones to excitant amino acids and acetylcholine. 2. The depression, which lasted for 15--30 min when ibotenate was ejected for 3--6 min, was blocked by the GABA-antagonist bicuculline methochloride, and was independent of prior firing since it occurred with subthreshold concentrations of ibotenate and when ibotenate firing had been blocked by DL-alpha-aminoadipate. 3. When administered electrophoretically for 5 min, muscimol, a potent GABA  agonist, reduced neuronal excitability for prolonged periods and this effect was also prevented by bicuculline methochloride. 4. The depression of neuronal excitability produced by GABA, taurine, isoguvacine or 3-aminopropane sulphonate, ejected for periods of 5--6 min, recovered rapidly. 5. It is suggested that ibotenate is converted in vivo to muscimol or a related compound which has a prolonged, bicuculline-sensitive depressant action on the excitability of neurones."}
{"id": "PMID:480206", "title": "Contribution of calcium and potassium permeability changes to the off response of scallop hyperpolarizing photoreceptors.", "content": "1. The membrane response of the distal photoreceptors in the retina of the scallop Pectin irradians to the termination of a bright white light (off response) is shown to be composed of the decay of the hyperpolarizing receptor potential and an action potential with slow kinetics. 2. The action potential can be produced in darkness in the absence of external Na+ ions by membrane depolarization. 3. The action potential is maintained by replacement of external Ca2+ with Sr2+ or Ba2+, but not by Mg2+. In normal external Ca2+ (9mM), the action potential is abolished by the addition of the Ca2+ inhibitors, La3+, Co2+, and Mn2+ or the organic Ca2+ antagonist D-600. 4. Elevated external Ca2+ concentrations increase the rate of rise and peak amplitude of the action potential as well as the rate of repolarization and after hyperpolarization, but decrease the duration. 5. The rate of rise and peak amplitude of the action potential are increased by the K+ antagonists tetraethylammonium (TEA) 4-amino-phyridine (4-AP), Ba2+ and procaine. The antagonists have different effects on subsequent phases of the response, however. External TEA and Ba2+ increase the duration, but decrease the rate of repolarization and abolish the after hyperpolarization, whereas external 4-AP and procaine increase the rate of repolarization, decrease the duration and increase the after hyperpolarization. 6. The ratio of the Ca2+ to K+ permeability (P Ca/P K) estimated from the constant field equation at the peak of the action potential in different external Ca2+ concentrations is close to 1. 7. The maximum rate of rise and the peak amplitude of the action potential are increased by membrane hyperpolarization and decreased by membrane depolarization. They are decreased by background light intensity relative to their value in the dark. 8. In normal ASW the action potential can be identified during the off response as a small overshoot of membrane potential relative to its value in the dark. 9. The rate of repolarization of the off response in normal ASW is reduced by agents or conditions which inhibit or reduce Ca2+ permeability changes, e.g. external Co2+ and La2+ or zero external Ca2+. 10. Our results suggest that a voltage-dependent increase in membrane permeability to Ca2+ and to K+ ions modifies the repolarizing phase of the receptor potential.", "contents": "Contribution of calcium and potassium permeability changes to the off response of scallop hyperpolarizing photoreceptors. 1. The membrane response of the distal photoreceptors in the retina of the scallop Pectin irradians to the termination of a bright white light (off response) is shown to be composed of the decay of the hyperpolarizing receptor potential and an action potential with slow kinetics. 2. The action potential can be produced in darkness in the absence of external Na+ ions by membrane depolarization. 3. The action potential is maintained by replacement of external Ca2+ with Sr2+ or Ba2+, but not by Mg2+. In normal external Ca2+ (9mM), the action potential is abolished by the addition of the Ca2+ inhibitors, La3+, Co2+, and Mn2+ or the organic Ca2+ antagonist D-600. 4. Elevated external Ca2+ concentrations increase the rate of rise and peak amplitude of the action potential as well as the rate of repolarization and after hyperpolarization, but decrease the duration. 5. The rate of rise and peak amplitude of the action potential are increased by the K+ antagonists tetraethylammonium (TEA) 4-amino-phyridine (4-AP), Ba2+ and procaine. The antagonists have different effects on subsequent phases of the response, however. External TEA and Ba2+ increase the duration, but decrease the rate of repolarization and abolish the after hyperpolarization, whereas external 4-AP and procaine increase the rate of repolarization, decrease the duration and increase the after hyperpolarization. 6. The ratio of the Ca2+ to K+ permeability (P Ca/P K) estimated from the constant field equation at the peak of the action potential in different external Ca2+ concentrations is close to 1. 7. The maximum rate of rise and the peak amplitude of the action potential are increased by membrane hyperpolarization and decreased by membrane depolarization. They are decreased by background light intensity relative to their value in the dark. 8. In normal ASW the action potential can be identified during the off response as a small overshoot of membrane potential relative to its value in the dark. 9. The rate of repolarization of the off response in normal ASW is reduced by agents or conditions which inhibit or reduce Ca2+ permeability changes, e.g. external Co2+ and La2+ or zero external Ca2+. 10. Our results suggest that a voltage-dependent increase in membrane permeability to Ca2+ and to K+ ions modifies the repolarizing phase of the receptor potential."}
{"id": "PMID:480210", "title": "The responses of afferent fibres from the glabrous skin of the hand during voluntary finger movements in man.", "content": "1. Afferent activity of 111 single units from the glabrous skin area was recorded percutaneously in the median nerve of human subjects, using tungsten electrodes. 2. The majority of the units (103) were classified as low-threshold mechano-sensitive units belonging to one of the four categories previously described: rapidly adapting with small receptive fields (RA), rapidly adapting with large receptive fields (PC, presumed Pacinian corpuscle units), slowly adapting with small fields (SA I), and slowly adapting with large fields (SA II). The size of the responses (in number of impulses) to indentation and stretching of the skin was compared with that of the responses elicited during voluntary isotonic finger movements, which avoided trivial excitation of the units by direct touch. 3. All four types of units, and 77% of the single units, were activated by isotonic movements. The decreasing order of responsiveness was PC, SA II, SA I, RA. 4. Almost all responsive units were excited during the dynamic phase of ramp and smooth oscillatory movements. Static responses, on the other hand, occurred only with 50% of the slowly adapting units, corresponding to a third of the total sample (SA II, 81%; SA I, 17%. 5. For all four types of units the dynamic responses to movements were of similar size as the responses to localized skin indentation with a von Frey hair at five times threshold. 6. The results are discussed with regard to the possible implications for kinaesthesia and motor control.", "contents": "The responses of afferent fibres from the glabrous skin of the hand during voluntary finger movements in man. 1. Afferent activity of 111 single units from the glabrous skin area was recorded percutaneously in the median nerve of human subjects, using tungsten electrodes. 2. The majority of the units (103) were classified as low-threshold mechano-sensitive units belonging to one of the four categories previously described: rapidly adapting with small receptive fields (RA), rapidly adapting with large receptive fields (PC, presumed Pacinian corpuscle units), slowly adapting with small fields (SA I), and slowly adapting with large fields (SA II). The size of the responses (in number of impulses) to indentation and stretching of the skin was compared with that of the responses elicited during voluntary isotonic finger movements, which avoided trivial excitation of the units by direct touch. 3. All four types of units, and 77% of the single units, were activated by isotonic movements. The decreasing order of responsiveness was PC, SA II, SA I, RA. 4. Almost all responsive units were excited during the dynamic phase of ramp and smooth oscillatory movements. Static responses, on the other hand, occurred only with 50% of the slowly adapting units, corresponding to a third of the total sample (SA II, 81%; SA I, 17%. 5. For all four types of units the dynamic responses to movements were of similar size as the responses to localized skin indentation with a von Frey hair at five times threshold. 6. The results are discussed with regard to the possible implications for kinaesthesia and motor control."}
{"id": "PMID:480212", "title": "On the separability of two mechanisms involved in the detection of grating patterns in humans.", "content": "1. The detectability of contrast modulation (M) of sinusoidal gratings was explored at the rate of 8 Hz. The luminance profile of a contrast modulated sinusoidal grating is L = L(1 + C cos 2 pi F chi). This stimulus may also be regarded as the sum of a steady grating pattern and counter phase flicker of the same spatial frequency. 2. Contrast modulation sensitivity (1/M) was established in five observers at several levels of constract and over a range of spatial frequencies, where M = delta C/C of delta C is the just detectable contrast change and C is the mean contrast of the grating. The slope of a modulation sensitivity function (C/delta C vs. C) is 1 (i.e. delta C = constant) near threshold contrast at each spatial frequency, but in the suprathreshold contrast range the slope flattens from close to 1 at 1.5 c/deg to almost 0 (delta C/C = constant) at 12 c/deg. 3. Adaptation to a high contrast steady grating of the same spatial frequency as the contrast modulated test gratings shifts each modulation sensitivity function to the right at low contrasts, but not at high. As a result the adapted curves cross their corresponding unadapted ones. At each spatial frequency the modulation sensitivity function is now fitted by a straight line of slope 1. While delta C needs to be higher than half the detection threshold of the same grating at spatial frequencies above 3 c/deg, in the adapted condition the values are nearly equal at each frequency. Thus pattern adaptation unmasks the threshold of the counterphase component of the contrast modulated grating near threshold contrast as well as above it. The phase of the steady adapting grating, relative to the steady component of the test grating, does not make any difference. 4. Apparently contrast modulation reveals differences beyond threshold sensitivity between spatial frequencies adjacent to the peak of the contrast sensitivity curve. For each spatial frequency channel there must be different neural coupling between steady and modulated inputs. Electrophysiological studies using contrast modulated gratings would be useful in the exploration of individual and ensemble properties of neurones of the visual cortex.", "contents": "On the separability of two mechanisms involved in the detection of grating patterns in humans. 1. The detectability of contrast modulation (M) of sinusoidal gratings was explored at the rate of 8 Hz. The luminance profile of a contrast modulated sinusoidal grating is L = L(1 + C cos 2 pi F chi). This stimulus may also be regarded as the sum of a steady grating pattern and counter phase flicker of the same spatial frequency. 2. Contrast modulation sensitivity (1/M) was established in five observers at several levels of constract and over a range of spatial frequencies, where M = delta C/C of delta C is the just detectable contrast change and C is the mean contrast of the grating. The slope of a modulation sensitivity function (C/delta C vs. C) is 1 (i.e. delta C = constant) near threshold contrast at each spatial frequency, but in the suprathreshold contrast range the slope flattens from close to 1 at 1.5 c/deg to almost 0 (delta C/C = constant) at 12 c/deg. 3. Adaptation to a high contrast steady grating of the same spatial frequency as the contrast modulated test gratings shifts each modulation sensitivity function to the right at low contrasts, but not at high. As a result the adapted curves cross their corresponding unadapted ones. At each spatial frequency the modulation sensitivity function is now fitted by a straight line of slope 1. While delta C needs to be higher than half the detection threshold of the same grating at spatial frequencies above 3 c/deg, in the adapted condition the values are nearly equal at each frequency. Thus pattern adaptation unmasks the threshold of the counterphase component of the contrast modulated grating near threshold contrast as well as above it. The phase of the steady adapting grating, relative to the steady component of the test grating, does not make any difference. 4. Apparently contrast modulation reveals differences beyond threshold sensitivity between spatial frequencies adjacent to the peak of the contrast sensitivity curve. For each spatial frequency channel there must be different neural coupling between steady and modulated inputs. Electrophysiological studies using contrast modulated gratings would be useful in the exploration of individual and ensemble properties of neurones of the visual cortex."}
{"id": "PMID:480214", "title": "Intraventricular microinjections of a stable analogue of prostaglandin endoperoxide cause fever in rabbits.", "content": "1. Derivatives of arachidonic acid other than prostaglandin are pyrogenic, the likely candidates being the prostaglandin endoperoxides and/or the thromboxanes. 2. Intraventricular microinjections in rabbits of a stable analogue of prostaglandin endoperoxide resulted in dose-dependent increases of rectal temperature. The pyrexia was delayed in onset; no significant change in body temperature occurred for at least an hour. 3. The pyrexia was unaltered by simultaneous injection of the potent prostaglandin synthetase inhibitor indomethacin. 4. We suggest that both prostaglandins and prostaglandin endoperoxides may be implicated in fever.", "contents": "Intraventricular microinjections of a stable analogue of prostaglandin endoperoxide cause fever in rabbits. 1. Derivatives of arachidonic acid other than prostaglandin are pyrogenic, the likely candidates being the prostaglandin endoperoxides and/or the thromboxanes. 2. Intraventricular microinjections in rabbits of a stable analogue of prostaglandin endoperoxide resulted in dose-dependent increases of rectal temperature. The pyrexia was delayed in onset; no significant change in body temperature occurred for at least an hour. 3. The pyrexia was unaltered by simultaneous injection of the potent prostaglandin synthetase inhibitor indomethacin. 4. We suggest that both prostaglandins and prostaglandin endoperoxides may be implicated in fever."}
{"id": "PMID:480216", "title": "Lysine transport across rat jejunum: distribution between the transcellular and the paracellular routes.", "content": "Transport of lysine across the rat jejunum has been studied measuring transmural fluxes, Jms and Jsm, under short-circuit conditions, influx across the brush-border membrane, Jmc, under open-circuit and voltage-clamp conditions, and steady-state uptake by the isolated mucosa. 1. Jlysmc can be described as the sum of a saturable process with a Kt of 3 mM and a Jmax of 2.25 micromole/cm2.hr and a diffusional component corresponding to a lysine permeability of 0.014 cm/hr. Also Jlysms is well described as the sum of a saturable process and a diffusional contribution described by the same permeability as for Jlysmc. 2. The effects of the transmural p.d. on Jlysmc indicate that at 60 mM this flux includes a diffusional contribution, which corresponds to a lysine permeability of 0.014 cm/hr. 3. The passage of an electrical current across the gut wall changes the electrical conductance as expected for a cation-selective epithelium. The effect of a mucosa to serosa current on the Jms value of mannitol provides confirmation of the expected current effect on transepithelial volume flow. These effects on conductance and solute flux, together with the electrostatic effect on lysine movements, suffice to account for the p.d. effects on Jmc, Jms, and Jsm of lysine. 4. Jlyssm is in a saturable manner stimulated by increasing concentrations of D-glucose. At higher (10 mM) concentrations of lysine this effect leads to a net secretion of lysine. Qualitatively and quantitatively these effects are consistent with the model of a glucose-induced fluid circuit between the mucosal solution and the lateral intercellular spaces. 5. All observations are consistent with a paracellular, transepithelial pathway for lysine, which includes the lateral intercellular spaces. 6. The transport of lysine across the basolateral membrane is analysed. Togethet the data on transcellular passage of lysine are very similar to those reported for rabbit ileum, except that more than one transport process could not be demonstrated.", "contents": "Lysine transport across rat jejunum: distribution between the transcellular and the paracellular routes. Transport of lysine across the rat jejunum has been studied measuring transmural fluxes, Jms and Jsm, under short-circuit conditions, influx across the brush-border membrane, Jmc, under open-circuit and voltage-clamp conditions, and steady-state uptake by the isolated mucosa. 1. Jlysmc can be described as the sum of a saturable process with a Kt of 3 mM and a Jmax of 2.25 micromole/cm2.hr and a diffusional component corresponding to a lysine permeability of 0.014 cm/hr. Also Jlysms is well described as the sum of a saturable process and a diffusional contribution described by the same permeability as for Jlysmc. 2. The effects of the transmural p.d. on Jlysmc indicate that at 60 mM this flux includes a diffusional contribution, which corresponds to a lysine permeability of 0.014 cm/hr. 3. The passage of an electrical current across the gut wall changes the electrical conductance as expected for a cation-selective epithelium. The effect of a mucosa to serosa current on the Jms value of mannitol provides confirmation of the expected current effect on transepithelial volume flow. These effects on conductance and solute flux, together with the electrostatic effect on lysine movements, suffice to account for the p.d. effects on Jmc, Jms, and Jsm of lysine. 4. Jlyssm is in a saturable manner stimulated by increasing concentrations of D-glucose. At higher (10 mM) concentrations of lysine this effect leads to a net secretion of lysine. Qualitatively and quantitatively these effects are consistent with the model of a glucose-induced fluid circuit between the mucosal solution and the lateral intercellular spaces. 5. All observations are consistent with a paracellular, transepithelial pathway for lysine, which includes the lateral intercellular spaces. 6. The transport of lysine across the basolateral membrane is analysed. Togethet the data on transcellular passage of lysine are very similar to those reported for rabbit ileum, except that more than one transport process could not be demonstrated."}
{"id": "PMID:480218", "title": "Assessment of the pulmonary origin of bronchoconstrictor vagal tone.", "content": "1. In anaesthetized spontaneously breathing cats, the sensory component of the vagal nerves was sectioned at the level of nodose ganglion, using a method described previously (Mei, 1966; Mei & Dussardier, 1966).2. The strength of the Hering-Breuer reflex (inhibitory ratio, i.e. T(1)/T(0)) provided a test for effectiveness of section of vagal afferents, particularly respiratory afferents. On the other hand, by studying the cardiac and bronchomotor effects induced by electrical stimulation of the supranodose portion of the vagal nerve, it was possible to test the integrity of the efferent vagal component.3. Unilateral right sensory vagotomy was followed by a 29% reduction in total pulmonary resistance.4. Section of the contralateral sensory vagal component (sensory bivagotomy), produced a weak supplementary effect (total decrease of total pulmonary resistance: 31%).5. No additive bronchomotor effect could be observed after the bilateral section of efferent vagal fibres (total bivagotomy).6. In intact cats, blockade of the two vagal nerves by procaine induced a decrease in pulmonary resistance similar to those produced by the sensory bivagotomy (23%). This bronchodilatator effect was concomitant with a complete disappearance of the C wave of the compound vagal potential.7. Intravenous injection of phenyl diguanide, immediately after the blockade of the C vagal fibres by procaine did not modify bronchomotor tone. This result confirms that the C pulmonary afferents, which are activated by phenyl diguanide, are mainly involved in this mechanism.8. The pulmonary irritant receptors seem to play a minor role. In fact, the I.V. administration of histamine under the same conditions, provides evidence that the corresponding neurones (small sized myelinated fibres) are potent during the procaine application.9. From these results, it appears that bronchoconstrictor vagal tone has an exclusive peripheral origin and that pulmonary endings, in particular those connected with non-medullated fibres, are probably involved in this mechanism.", "contents": "Assessment of the pulmonary origin of bronchoconstrictor vagal tone. 1. In anaesthetized spontaneously breathing cats, the sensory component of the vagal nerves was sectioned at the level of nodose ganglion, using a method described previously (Mei, 1966; Mei & Dussardier, 1966).2. The strength of the Hering-Breuer reflex (inhibitory ratio, i.e. T(1)/T(0)) provided a test for effectiveness of section of vagal afferents, particularly respiratory afferents. On the other hand, by studying the cardiac and bronchomotor effects induced by electrical stimulation of the supranodose portion of the vagal nerve, it was possible to test the integrity of the efferent vagal component.3. Unilateral right sensory vagotomy was followed by a 29% reduction in total pulmonary resistance.4. Section of the contralateral sensory vagal component (sensory bivagotomy), produced a weak supplementary effect (total decrease of total pulmonary resistance: 31%).5. No additive bronchomotor effect could be observed after the bilateral section of efferent vagal fibres (total bivagotomy).6. In intact cats, blockade of the two vagal nerves by procaine induced a decrease in pulmonary resistance similar to those produced by the sensory bivagotomy (23%). This bronchodilatator effect was concomitant with a complete disappearance of the C wave of the compound vagal potential.7. Intravenous injection of phenyl diguanide, immediately after the blockade of the C vagal fibres by procaine did not modify bronchomotor tone. This result confirms that the C pulmonary afferents, which are activated by phenyl diguanide, are mainly involved in this mechanism.8. The pulmonary irritant receptors seem to play a minor role. In fact, the I.V. administration of histamine under the same conditions, provides evidence that the corresponding neurones (small sized myelinated fibres) are potent during the procaine application.9. From these results, it appears that bronchoconstrictor vagal tone has an exclusive peripheral origin and that pulmonary endings, in particular those connected with non-medullated fibres, are probably involved in this mechanism."}
{"id": "PMID:480220", "title": "Intracellular citrate or externaly applied tetraethylammonium ions produce calcium-dependent action potentials in an insect motoneurone cell body.", "content": "1. Electrophysiological observations have been made upon the cell body of an identified motoneurone of the cockroach, Periplaneta americana. Normal responses were compared with those observed after intracellular injection of citrate anions or when the preparation was bathed in solutions containing tetraethylammonium ions (TEA+).2. Normally when depolarized, the motoneurone soma gave a series of damped oscillations; the amplitude of these responses increased with increase in the applied current.3. After citrate ions had been injected into the neurone soma, all-or-none action potentials were evoked by depolarization; such responses appeared about 5-10 min after the onset of citrate injection. Injection of EGTA produced similar effects. Citrate and EGTA probably produce their effect through a reduction in the intracellular free calcium concentration.4. When preparations were bathed in saline solution containing 50 mM-TEA+, soma depolarization produced prolonged all-or-none action potentials (up to approximately 100 msec duration).5. The action potentials produced by citrate injection or externally applied TEA+ appeared to have a similar ionic mechanism; they were not depressed by sodium-free solutions or by tetrodotoxin (4 x 10(-6)M) but were reversibly blocked in saline solution containing 40 mM-manganous chloride.6. The overshoot amplitude of action potentials recorded after injection of citrate anions or in solutions containing TEA+ showed a 22.5 mV change for a ten-fold change in the external calcium concentration.7. Both intracellular citrate and external TEA+ caused a significant increase in the input resistance and membrane time constant of the motoneurone.8. It is concluded that action potentials generated under various experimental conditions in the soma of this insect motoneurone map have differing ionic mechanisms.", "contents": "Intracellular citrate or externaly applied tetraethylammonium ions produce calcium-dependent action potentials in an insect motoneurone cell body. 1. Electrophysiological observations have been made upon the cell body of an identified motoneurone of the cockroach, Periplaneta americana. Normal responses were compared with those observed after intracellular injection of citrate anions or when the preparation was bathed in solutions containing tetraethylammonium ions (TEA+).2. Normally when depolarized, the motoneurone soma gave a series of damped oscillations; the amplitude of these responses increased with increase in the applied current.3. After citrate ions had been injected into the neurone soma, all-or-none action potentials were evoked by depolarization; such responses appeared about 5-10 min after the onset of citrate injection. Injection of EGTA produced similar effects. Citrate and EGTA probably produce their effect through a reduction in the intracellular free calcium concentration.4. When preparations were bathed in saline solution containing 50 mM-TEA+, soma depolarization produced prolonged all-or-none action potentials (up to approximately 100 msec duration).5. The action potentials produced by citrate injection or externally applied TEA+ appeared to have a similar ionic mechanism; they were not depressed by sodium-free solutions or by tetrodotoxin (4 x 10(-6)M) but were reversibly blocked in saline solution containing 40 mM-manganous chloride.6. The overshoot amplitude of action potentials recorded after injection of citrate anions or in solutions containing TEA+ showed a 22.5 mV change for a ten-fold change in the external calcium concentration.7. Both intracellular citrate and external TEA+ caused a significant increase in the input resistance and membrane time constant of the motoneurone.8. It is concluded that action potentials generated under various experimental conditions in the soma of this insect motoneurone map have differing ionic mechanisms."}
{"id": "PMID:480225", "title": "Discharge patterns of Purkinje cells in cats anaesthetized with alpha-chloralose.", "content": "1. Micro-electrodes have been used to record from 119 Purkinje (P) cells in the paramedian lobule of the cerebellum in cats anaesthetized with alpha-chloralose. 2. The spontaneous discharge rate and degree of irregularity of the discharge varied very much from one cell to another; the over-all mean rate (about 25/sec) was a little lower than has been reported either for barbiturate anaesthetized or for decerebrate unanaesthetized preparations. 3. Following electrical stimulation of a peripheral nerve, most P cells responded with both simple spikes and a climbing fibre response. This initial response was usually succeeded by a prolonged period of silence (over-all mean duration 350 msec) before resumption of the tonic simple spike discharge. Similar response-silence sequences could also be evoked by mechanical stimulation such as a tap applied to the pads of the forepaw. 4. Electrical stimulation of the inferior olive evoked climbing fibre responses followed by a prolonged pause in the simple spike discharge of the cell. 5. In six individual preparations, recordings were made both from P cells of the paramedian lobule and from neurones of nucleus interpositus (to which the former project). Comparison of the responses of the two types of neurone to peripheral nerve and inferior olivary stimulation showed that the end of the pauses in P cell firing correlated well with the end of a prolonged period of facilitation of the interpositus neurones. 6. These results support the hypothesis advanced in an earlier report (Armstrong, Cogdell & Harvey, 1975) that the prolonged facilitatory responses of interpositus neurones are essentially disinhibitory responses resulting from reduction in the activity of overlying cells, and that responses of P cells and of interpositus neurones consist, in general, of modulations of activity which are mutually out of phase.", "contents": "Discharge patterns of Purkinje cells in cats anaesthetized with alpha-chloralose. 1. Micro-electrodes have been used to record from 119 Purkinje (P) cells in the paramedian lobule of the cerebellum in cats anaesthetized with alpha-chloralose. 2. The spontaneous discharge rate and degree of irregularity of the discharge varied very much from one cell to another; the over-all mean rate (about 25/sec) was a little lower than has been reported either for barbiturate anaesthetized or for decerebrate unanaesthetized preparations. 3. Following electrical stimulation of a peripheral nerve, most P cells responded with both simple spikes and a climbing fibre response. This initial response was usually succeeded by a prolonged period of silence (over-all mean duration 350 msec) before resumption of the tonic simple spike discharge. Similar response-silence sequences could also be evoked by mechanical stimulation such as a tap applied to the pads of the forepaw. 4. Electrical stimulation of the inferior olive evoked climbing fibre responses followed by a prolonged pause in the simple spike discharge of the cell. 5. In six individual preparations, recordings were made both from P cells of the paramedian lobule and from neurones of nucleus interpositus (to which the former project). Comparison of the responses of the two types of neurone to peripheral nerve and inferior olivary stimulation showed that the end of the pauses in P cell firing correlated well with the end of a prolonged period of facilitation of the interpositus neurones. 6. These results support the hypothesis advanced in an earlier report (Armstrong, Cogdell & Harvey, 1975) that the prolonged facilitatory responses of interpositus neurones are essentially disinhibitory responses resulting from reduction in the activity of overlying cells, and that responses of P cells and of interpositus neurones consist, in general, of modulations of activity which are mutually out of phase."}
{"id": "PMID:480226", "title": "Functional characteristics of slowly adapting pulmonary stretch receptors in the turtle (Chrysemys picta).", "content": "1. Single nerve fibre discharge has been recorded from slowly adapting pulmonary stretch receptors in single-pithed turtles on artificial ventilation. 2. Receptor discharge during static and dynamic lung inflations showed that lung volume was the major stimulus of these receptors. The rate and degree of change in transpulmonary pressure were without direct effect. 3. The response of these receptors to static and dynamic lung inflation differed only quantitatively from those of bronchopulmonary stretch receptors in mammals. The lower discharge frequencies and sensitivities of the turtle receptors may arise from the low body temperature of these animals. 4. The sensitivity of turtle pulmonary receptors to CO2 was greater than that recorded for bronchopulmonary receptors of mammals although the effects of CO2 on receptor discharge were qualitatively similar. In several instances, receptor discharge was totally inhibited throughout the ventilatory cycle by inflation with 5--10% CO2 in air.", "contents": "Functional characteristics of slowly adapting pulmonary stretch receptors in the turtle (Chrysemys picta). 1. Single nerve fibre discharge has been recorded from slowly adapting pulmonary stretch receptors in single-pithed turtles on artificial ventilation. 2. Receptor discharge during static and dynamic lung inflations showed that lung volume was the major stimulus of these receptors. The rate and degree of change in transpulmonary pressure were without direct effect. 3. The response of these receptors to static and dynamic lung inflation differed only quantitatively from those of bronchopulmonary stretch receptors in mammals. The lower discharge frequencies and sensitivities of the turtle receptors may arise from the low body temperature of these animals. 4. The sensitivity of turtle pulmonary receptors to CO2 was greater than that recorded for bronchopulmonary receptors of mammals although the effects of CO2 on receptor discharge were qualitatively similar. In several instances, receptor discharge was totally inhibited throughout the ventilatory cycle by inflation with 5--10% CO2 in air."}
{"id": "PMID:480230", "title": "The nature of the atrial receptors responsible for a reflex increase in heart rate in the dog.", "content": "1. In dogs anaesthetized with chloralose, small latex balloons were positioned at the left pulmonary vein-atrial junctions so as to stretch this region. By recording action potentials from slips of the cervical vagi it was established that distension of these balloons stimulated receptor endings in the atrial endocardium which discharged into the myelinated branches of the vagi i.e. Paintal type A, type B and Intermediate type receptors. 2. In other dogs, cooling the cervical vagus in steps of 2 degrees C reduced this response in vagal myelinated fibres. With twelve receptors the response to distension was reduced by 30% when the vagus was cooled to 16 degrees C, by 70% when cooled to 12 degrees C and abolished at 8--12 degrees C. 3. In a third group of dogs, distension of balloons at the pulmonary vein-atrial junctions resulted in a reflex increase in heart rate. Cooling the cervical vagi in these dogs in stages to 8 degrees C reduced this increase in heart rate. In nine dogs the response was reduced by 20% when the vagi was cooled to 16 degrees C, by 70% when cooled to 12 degrees C and abolished between 12 and 8 degrees C. 4. In a fourth group of dogs, distension of balloons at the pulmonary vein-atrial junctions was shown also to activate receptor endings in the atria which discharged into non-myelinated branches of the Vagi. In twelve receptors, cooling the cervical vagus in steps of 2 degrees C reduced this evoked increase in activity in non-myelinated fibres. These responses were abolished over a wide range of temperature unlike the responses observed above. 5. It is concluded that the increase in heart rate which follows distension of balloons at the pulmonary vein-atrial junctions is mediated solely by the Paintal-type receptors which discharge into the myelinated fibres in the vagi.", "contents": "The nature of the atrial receptors responsible for a reflex increase in heart rate in the dog. 1. In dogs anaesthetized with chloralose, small latex balloons were positioned at the left pulmonary vein-atrial junctions so as to stretch this region. By recording action potentials from slips of the cervical vagi it was established that distension of these balloons stimulated receptor endings in the atrial endocardium which discharged into the myelinated branches of the vagi i.e. Paintal type A, type B and Intermediate type receptors. 2. In other dogs, cooling the cervical vagus in steps of 2 degrees C reduced this response in vagal myelinated fibres. With twelve receptors the response to distension was reduced by 30% when the vagus was cooled to 16 degrees C, by 70% when cooled to 12 degrees C and abolished at 8--12 degrees C. 3. In a third group of dogs, distension of balloons at the pulmonary vein-atrial junctions resulted in a reflex increase in heart rate. Cooling the cervical vagi in these dogs in stages to 8 degrees C reduced this increase in heart rate. In nine dogs the response was reduced by 20% when the vagi was cooled to 16 degrees C, by 70% when cooled to 12 degrees C and abolished between 12 and 8 degrees C. 4. In a fourth group of dogs, distension of balloons at the pulmonary vein-atrial junctions was shown also to activate receptor endings in the atria which discharged into non-myelinated branches of the Vagi. In twelve receptors, cooling the cervical vagus in steps of 2 degrees C reduced this evoked increase in activity in non-myelinated fibres. These responses were abolished over a wide range of temperature unlike the responses observed above. 5. It is concluded that the increase in heart rate which follows distension of balloons at the pulmonary vein-atrial junctions is mediated solely by the Paintal-type receptors which discharge into the myelinated fibres in the vagi."}
{"id": "PMID:480233", "title": "Gastric emptying and secretion in the calf on duodenal infusion of tryptophan, tryptamine and 5-hydroxytryptamine.", "content": "1. Gastric emptying, gastric acid and pepsinogen secretion were assessed simultaneously in the conscious calf using the test meal and duodenal perfusion technique (Bell & Mostaghni, 1975).2. When 60 mM-HCl was infused into the duodenum, gastric emptying was arrested but both acid and pepsinogen secretion continued at a low level. Duodenal infusion with isotonic NaHCO(3) caused rapid exponential emptying of the test meal and acid and pepsinogen output was more than doubled.3. Duodenal infusion of amino acids in isotonic NaHCO(3) did not affect the rapid emptying, except infusion with tryptophan, which caused a measureable degree of inhibition of emptying, with concomitant effects on acid and pepsinogen secretion4. Tryptamine and 5-hydroxytryptamine (5-HT) incorporated in low concentration into isotonic NaHCO(3) also produced depression of gastric emptying, acid and pepsinogen levels comparable to the response initiated by acid infusate. Tryptophan was effective only in non-physiological amounts while 5-HT and tryptamine were active in smaller doses.5. Our results suggest that the inhibition of gastric emptying following duodenal infusion of tryptophan noted by Stephens, Woolson & Cooke (1975) may be due to the duodenal synthesis of its biogenic amine derivatives tryptamine and 5-HT.6. The level of activity of the three gastric functions, emptying, acid secretion and pepsinogen secretion appears to be linked. A single stimulus, therefore, could evoke a duodenal receptor or receptors to mediate or suppress activity of the gastric smooth muscle and secretory cells through interrelated mechanisms. The effect of some duodenal infusates, however, produces some variability in response which suggests differential activation of different receptors with consequent variable motor activity on effector cells.", "contents": "Gastric emptying and secretion in the calf on duodenal infusion of tryptophan, tryptamine and 5-hydroxytryptamine. 1. Gastric emptying, gastric acid and pepsinogen secretion were assessed simultaneously in the conscious calf using the test meal and duodenal perfusion technique (Bell & Mostaghni, 1975).2. When 60 mM-HCl was infused into the duodenum, gastric emptying was arrested but both acid and pepsinogen secretion continued at a low level. Duodenal infusion with isotonic NaHCO(3) caused rapid exponential emptying of the test meal and acid and pepsinogen output was more than doubled.3. Duodenal infusion of amino acids in isotonic NaHCO(3) did not affect the rapid emptying, except infusion with tryptophan, which caused a measureable degree of inhibition of emptying, with concomitant effects on acid and pepsinogen secretion4. Tryptamine and 5-hydroxytryptamine (5-HT) incorporated in low concentration into isotonic NaHCO(3) also produced depression of gastric emptying, acid and pepsinogen levels comparable to the response initiated by acid infusate. Tryptophan was effective only in non-physiological amounts while 5-HT and tryptamine were active in smaller doses.5. Our results suggest that the inhibition of gastric emptying following duodenal infusion of tryptophan noted by Stephens, Woolson & Cooke (1975) may be due to the duodenal synthesis of its biogenic amine derivatives tryptamine and 5-HT.6. The level of activity of the three gastric functions, emptying, acid secretion and pepsinogen secretion appears to be linked. A single stimulus, therefore, could evoke a duodenal receptor or receptors to mediate or suppress activity of the gastric smooth muscle and secretory cells through interrelated mechanisms. The effect of some duodenal infusates, however, produces some variability in response which suggests differential activation of different receptors with consequent variable motor activity on effector cells."}
{"id": "PMID:480234", "title": "Inhibition of gamma motoneurone discharge by contraction of the homonymous muscle in the decerebrated cat.", "content": "1. The aim of this study has been to determine the autogenetic reflex effects of tendon organ stimulation upon gamma motoneurones in the decerebrated cat. 2. Tendon organs serving a muscle were excited by isometric twitch contractions of some motor units of that muscle. Contractions were elicited by electrical stimulation of the cut peripheral end of part of the ventral root supply, using a stimulus strength at or below maximum for alpha motoneurone axons. Afferent recordings in the same cats as were used to study reflexes of gamma motoneurones showed that tendon organs were powerfully excited during twitch contractions of their muscle. Early discharges were also elicited in both tendon organs and spindle primary afferents. 3. Background discharges of forty-seven triceps surae gamma motoneurones, ranging in axonal conduction velocity from 17 to 40 m/sec, were recorded from filaments of the muscle nerve. The initial response of twenty-two (47%) neurones to twitches of the triceps surae was inhibition of their discharge. The latency from time of stimulation of the ventral root ranged from 10 to 22 msec. The lowered probability of firing lasted an average of 25 msec and, occasionally, was seen as a short silent period. 4. The inhibition was reduced in duration by decreasing the resting muscle length so that less active tension was developed during contraction. 5. The inhibition was observed, in one instance, in the spinally transected decerebrated cat. 6. We argue the case that early discharges in tendon organ afferents give rise to the onset of inhibition and that tension dependent discharges of tendon organs contribute further inhibition of gamma motoneurones. 7. Sixteen gamma motoneurones were not affected by contraction but seventeen showed a period of facilitation. The facilitation could occur after a period of inhibition (eight neurones) or as the sole response to contraction. In either case facilitation occurred mainly during relaxation of the muscle and may, in part, be attributable to increased spindle primary ending firing at this time. 8. In discussion a comparision is made of the reflex effects of muscle afferent discharges on alpha and gamma motoneurones.", "contents": "Inhibition of gamma motoneurone discharge by contraction of the homonymous muscle in the decerebrated cat. 1. The aim of this study has been to determine the autogenetic reflex effects of tendon organ stimulation upon gamma motoneurones in the decerebrated cat. 2. Tendon organs serving a muscle were excited by isometric twitch contractions of some motor units of that muscle. Contractions were elicited by electrical stimulation of the cut peripheral end of part of the ventral root supply, using a stimulus strength at or below maximum for alpha motoneurone axons. Afferent recordings in the same cats as were used to study reflexes of gamma motoneurones showed that tendon organs were powerfully excited during twitch contractions of their muscle. Early discharges were also elicited in both tendon organs and spindle primary afferents. 3. Background discharges of forty-seven triceps surae gamma motoneurones, ranging in axonal conduction velocity from 17 to 40 m/sec, were recorded from filaments of the muscle nerve. The initial response of twenty-two (47%) neurones to twitches of the triceps surae was inhibition of their discharge. The latency from time of stimulation of the ventral root ranged from 10 to 22 msec. The lowered probability of firing lasted an average of 25 msec and, occasionally, was seen as a short silent period. 4. The inhibition was reduced in duration by decreasing the resting muscle length so that less active tension was developed during contraction. 5. The inhibition was observed, in one instance, in the spinally transected decerebrated cat. 6. We argue the case that early discharges in tendon organ afferents give rise to the onset of inhibition and that tension dependent discharges of tendon organs contribute further inhibition of gamma motoneurones. 7. Sixteen gamma motoneurones were not affected by contraction but seventeen showed a period of facilitation. The facilitation could occur after a period of inhibition (eight neurones) or as the sole response to contraction. In either case facilitation occurred mainly during relaxation of the muscle and may, in part, be attributable to increased spindle primary ending firing at this time. 8. In discussion a comparision is made of the reflex effects of muscle afferent discharges on alpha and gamma motoneurones."}
{"id": "PMID:480237", "title": "Relationship between calcium release and potassium release in rat parotid gland.", "content": "1. A study was made of the role of Ca release in mediating the sustained phase of K release (86 Rb release) in the rat parotid gland due to receptor activation. 2. Sr could substitute for Ca in supporting the sustained phase of the 86Rb release due to carbachol. Sr was less effective than Ca, while Ba was ineffective. 3. Sr could also restore responsiveness of the transient phase to agonists in media lacking Ca. Again, Sr was less effective than Ca, and Ba was without effect. 4. Carbachol stimulated release of 45Ca from parotid gland slices. The amount of Ca released (about 0.2 micromole/g) was much greater than the estimated Ca influx for the same period. 5. Release of 45Ca by carbachol prevented release due to a subsequent exposure to substance P. 6. Release of 45Ca due to substance P was significantly increased if muscarinic receptors were activated during tissue labelling. 7. These results support an earlier hypothesis whereby different agonists (such as carbachol and substance P) stimulate the release of Ca from a common pool and the release of this Ca mediates the transient phase of the K release response.", "contents": "Relationship between calcium release and potassium release in rat parotid gland. 1. A study was made of the role of Ca release in mediating the sustained phase of K release (86 Rb release) in the rat parotid gland due to receptor activation. 2. Sr could substitute for Ca in supporting the sustained phase of the 86Rb release due to carbachol. Sr was less effective than Ca, while Ba was ineffective. 3. Sr could also restore responsiveness of the transient phase to agonists in media lacking Ca. Again, Sr was less effective than Ca, and Ba was without effect. 4. Carbachol stimulated release of 45Ca from parotid gland slices. The amount of Ca released (about 0.2 micromole/g) was much greater than the estimated Ca influx for the same period. 5. Release of 45Ca by carbachol prevented release due to a subsequent exposure to substance P. 6. Release of 45Ca due to substance P was significantly increased if muscarinic receptors were activated during tissue labelling. 7. These results support an earlier hypothesis whereby different agonists (such as carbachol and substance P) stimulate the release of Ca from a common pool and the release of this Ca mediates the transient phase of the K release response."}
{"id": "PMID:480239", "title": "Sodium and calcium gating currents in an Aplysia neurone.", "content": "1. Currents generated by depolarizing the hyperpolarizing voltage pulses were recorded at temperatures of 4--12 degrees C in the voltage-clamped soma of R15 in aplysia abdominal ganglia exposed to solutions which suppressed ionic currents. 2. Subtraction of linear capacitive and leakage currents from current generated by voltage pulses to levels more positive than -20mV revealed non-linear transient outward displacement currents at the onset of the clamp step (on-current) and transient inward displacement currents after the membrane potential returned to the holding potential (off-current). Only on-currents were studied. 3. Pulses to membrane potentials of -20 to 0 mV generated a displacement current with rapid onset and exponential decay. At membrane potentials more positive than o mV a second displacement current with a much slower onset and slower exponential decay was seen. Because the different threshold potentials for the two displacement currents were close to the different threshold potentials for Na and Ca ion currents, the two displacement currents were called Na and Ca 'gating' currents. 4. The amount of charge transfer during Ca gating currents increased sigmoidally with increasing depolarization, reaching a maximum at +30 to +40 mV. Half-maximum charge transfer occurred at +15 mV. 5. Total charge movement during Ca gating currents was maximal with holding potentials of -30 to -40 mV. More positive or more negative holding potentials produced a decrease in charge movement. 6. The time course of the gating currents, but not the total charge displaced, was very sensitive to temperature. The time constant of decay of Ca gating currents had a Q10 of about 3, whereas the total amount of charge displaced had a Q10 of 1.2. 7. The charge transfer during both Na and Ca gating currents and the amplitude of Na and Ca (but not K) ionic currents were reduced in solutions containing 1 mm-n-octanol.", "contents": "Sodium and calcium gating currents in an Aplysia neurone. 1. Currents generated by depolarizing the hyperpolarizing voltage pulses were recorded at temperatures of 4--12 degrees C in the voltage-clamped soma of R15 in aplysia abdominal ganglia exposed to solutions which suppressed ionic currents. 2. Subtraction of linear capacitive and leakage currents from current generated by voltage pulses to levels more positive than -20mV revealed non-linear transient outward displacement currents at the onset of the clamp step (on-current) and transient inward displacement currents after the membrane potential returned to the holding potential (off-current). Only on-currents were studied. 3. Pulses to membrane potentials of -20 to 0 mV generated a displacement current with rapid onset and exponential decay. At membrane potentials more positive than o mV a second displacement current with a much slower onset and slower exponential decay was seen. Because the different threshold potentials for the two displacement currents were close to the different threshold potentials for Na and Ca ion currents, the two displacement currents were called Na and Ca 'gating' currents. 4. The amount of charge transfer during Ca gating currents increased sigmoidally with increasing depolarization, reaching a maximum at +30 to +40 mV. Half-maximum charge transfer occurred at +15 mV. 5. Total charge movement during Ca gating currents was maximal with holding potentials of -30 to -40 mV. More positive or more negative holding potentials produced a decrease in charge movement. 6. The time course of the gating currents, but not the total charge displaced, was very sensitive to temperature. The time constant of decay of Ca gating currents had a Q10 of about 3, whereas the total amount of charge displaced had a Q10 of 1.2. 7. The charge transfer during both Na and Ca gating currents and the amplitude of Na and Ca (but not K) ionic currents were reduced in solutions containing 1 mm-n-octanol."}
{"id": "PMID:480244", "title": "Inactivation of delayed outward current in molluscan neurone somata.", "content": "1. Inactivation of delayed outward current was studied by voltage clamp of isolated neurone somata of the molluscs Archidoris and Anisodoris. During prolonged voltage clamp steps in normal artificial sea water delayed outward current rises to a peak and then declines to a non-zero steady-state. During repetitive clamp pulses at repetition rates slower than 2/sec, the amplitude of peak outward current in the second pulse is commonly less than the amplitude at the end of the preceding pulse, giving the impression of continued inactivation during the repolarized interval. We have termed this property cumulative inactivation. 2. Two components of delayed outward current were separated using tetraethyl ammonium ions (TEA) and cobalt ions (Co). External TEA blocks 90% of a voltage and time dependent outward current termed K current (IK). External Co blocks 85% of a Ca activated delayed outward current termed Ca current (ICa does not inactivate during prolonged or repetitive voltage clamp pulses. IK, however, inactivates during prolonged voltage clamp steps and shows cumulative inactivation during repetitive voltage clamp pulses. 3. Inactivation of IK is voltage and time dependent and does not require influx of Ca ions. 4. As measured by a prepulse method, the onset of inactivation is characterized by a two time constant process. Fast inactivation occurs with a time course comparable to the rate of rise of outward current and can account for 90% of total inactivation. 5. Recovery from inactivation is slow with a time constant approximately an order of magnitude slower than the onset of inactivation. 6. The current-voltage (I-V) curve for peak IK can be N-shaped, with a region of negative slope resistance in the range of +30 to +80 mV. The I-V curve for steady-state IK, however, shows little or no tendency to form a local maximum. 7. The pattern of delayed outward current varies considerably between cells. A major contributing factor to this variability appears to be the relative contributions of ICa and IK to delayed outward current.", "contents": "Inactivation of delayed outward current in molluscan neurone somata. 1. Inactivation of delayed outward current was studied by voltage clamp of isolated neurone somata of the molluscs Archidoris and Anisodoris. During prolonged voltage clamp steps in normal artificial sea water delayed outward current rises to a peak and then declines to a non-zero steady-state. During repetitive clamp pulses at repetition rates slower than 2/sec, the amplitude of peak outward current in the second pulse is commonly less than the amplitude at the end of the preceding pulse, giving the impression of continued inactivation during the repolarized interval. We have termed this property cumulative inactivation. 2. Two components of delayed outward current were separated using tetraethyl ammonium ions (TEA) and cobalt ions (Co). External TEA blocks 90% of a voltage and time dependent outward current termed K current (IK). External Co blocks 85% of a Ca activated delayed outward current termed Ca current (ICa does not inactivate during prolonged or repetitive voltage clamp pulses. IK, however, inactivates during prolonged voltage clamp steps and shows cumulative inactivation during repetitive voltage clamp pulses. 3. Inactivation of IK is voltage and time dependent and does not require influx of Ca ions. 4. As measured by a prepulse method, the onset of inactivation is characterized by a two time constant process. Fast inactivation occurs with a time course comparable to the rate of rise of outward current and can account for 90% of total inactivation. 5. Recovery from inactivation is slow with a time constant approximately an order of magnitude slower than the onset of inactivation. 6. The current-voltage (I-V) curve for peak IK can be N-shaped, with a region of negative slope resistance in the range of +30 to +80 mV. The I-V curve for steady-state IK, however, shows little or no tendency to form a local maximum. 7. The pattern of delayed outward current varies considerably between cells. A major contributing factor to this variability appears to be the relative contributions of ICa and IK to delayed outward current."}
{"id": "PMID:480247", "title": "Mechanism of frequency-dependent broadening of molluscan neurone soma spikes.", "content": "1. Action potentials recorded from isolated dorid neurone somata increase in duration, i.e. broaden, during low frequency repetitive firing. Spike broadening is substantially reduced by external Co ions and implicates an inward Ca current. 2. During repetitive voltage clamp steps at frequencies slower than 1 Hz, in 100 mM-tetraethyl ammonium ions (TEA) inward Ca currents do not increase in amplitude. 3. Repetitive action potentials result in inactivation of delayed outward current. Likewise, repetitive voltage clamp steps which cause inactivation of delayed outward current also result in longer duration action potentials. 4. The frequency dependence of spike broadening and inactivation of the voltage dependent component (IK) of delayed outward current are similar. 5. Inactivation of IK is observed in all cells, however, only cells with relative large inward Ca currents show significant spike broadening. Spike broadening apparently results from the frequency dependent inactivation of IK which increases the expression of inward Ca current as a prominent shoulder on the repolarizing phase of the action potential. In addition, the presence of a prolonged Ca current increases the duration of the first action potential thereby allowing sufficient time for inactivation of IK.", "contents": "Mechanism of frequency-dependent broadening of molluscan neurone soma spikes. 1. Action potentials recorded from isolated dorid neurone somata increase in duration, i.e. broaden, during low frequency repetitive firing. Spike broadening is substantially reduced by external Co ions and implicates an inward Ca current. 2. During repetitive voltage clamp steps at frequencies slower than 1 Hz, in 100 mM-tetraethyl ammonium ions (TEA) inward Ca currents do not increase in amplitude. 3. Repetitive action potentials result in inactivation of delayed outward current. Likewise, repetitive voltage clamp steps which cause inactivation of delayed outward current also result in longer duration action potentials. 4. The frequency dependence of spike broadening and inactivation of the voltage dependent component (IK) of delayed outward current are similar. 5. Inactivation of IK is observed in all cells, however, only cells with relative large inward Ca currents show significant spike broadening. Spike broadening apparently results from the frequency dependent inactivation of IK which increases the expression of inward Ca current as a prominent shoulder on the repolarizing phase of the action potential. In addition, the presence of a prolonged Ca current increases the duration of the first action potential thereby allowing sufficient time for inactivation of IK."}
{"id": "PMID:480253", "title": "The role of circulating renin in drinking in response to isoprenaline.", "content": "1. In nephrectomized rats, S.C. (0.12 mg. kg body wt.(-1)) or intracerebroventricular (I.C.V.: 0.03 mg. kg(-1)), isoprenaline failed to elicit drinking. However, when preceded (5-20 min) by a non-dipsogenic dose of I.V. pig renin, S.C. isoprenaline induced a marked, and I.C.V. isoprenaline a smaller drinking response. 2 hr after I.V. renin, S.C. isoprenaline no longer caused drinking.2. Pig renin did not enhance drinking in response to 0.12 mg. kg(-1) isoprenaline S.C. in intact or sham-operated rats.3. Isoprenaline (0.12 mg. kg body wt.(-1), S.C.) caused a larger fall of blood pressure in unanaesthetized nephrectomized than in intact unanaesthetized rats, but it was not the resulting hypotension that interfered with the nephrectomized rats' ability to drink, since intact rats with similar falls in blood pressure drank avidly in response to large doses of isoprenaline.4. Since the rate of inactivation of pig renin in nephrectomized rats was not modified by isoprenaline, drinking in nephrectomized animals in response to renin+isoprenaline was not attributable to increased plasma renin levels.5. Since isoprenaline induces drinking in the presence of circulating renin, but in the absence of renin release from kidneys, renin plays a permissive role in isoprenaline-induced drinking. Angiotensin and isoprenaline may interact at the level of intracranial receptors.", "contents": "The role of circulating renin in drinking in response to isoprenaline. 1. In nephrectomized rats, S.C. (0.12 mg. kg body wt.(-1)) or intracerebroventricular (I.C.V.: 0.03 mg. kg(-1)), isoprenaline failed to elicit drinking. However, when preceded (5-20 min) by a non-dipsogenic dose of I.V. pig renin, S.C. isoprenaline induced a marked, and I.C.V. isoprenaline a smaller drinking response. 2 hr after I.V. renin, S.C. isoprenaline no longer caused drinking.2. Pig renin did not enhance drinking in response to 0.12 mg. kg(-1) isoprenaline S.C. in intact or sham-operated rats.3. Isoprenaline (0.12 mg. kg body wt.(-1), S.C.) caused a larger fall of blood pressure in unanaesthetized nephrectomized than in intact unanaesthetized rats, but it was not the resulting hypotension that interfered with the nephrectomized rats' ability to drink, since intact rats with similar falls in blood pressure drank avidly in response to large doses of isoprenaline.4. Since the rate of inactivation of pig renin in nephrectomized rats was not modified by isoprenaline, drinking in nephrectomized animals in response to renin+isoprenaline was not attributable to increased plasma renin levels.5. Since isoprenaline induces drinking in the presence of circulating renin, but in the absence of renin release from kidneys, renin plays a permissive role in isoprenaline-induced drinking. Angiotensin and isoprenaline may interact at the level of intracranial receptors."}
{"id": "PMID:480264", "title": "Achievement, aggression, and perceived adult age stages.", "content": "A questionnaire was administered to 173 male and female undergraduates to contrast their perceptions of the transition periods of young, middle age, and old adulthood stages of aging. A comparison of the perceived stages was then made with the personality dimensions of achievement and aggression. A 2 X 2 X 3 analysis of variance was computed for sex of S, sex of the perceived individual, and three adult stages. In addition, coefficients of correlation were obtained for 24 variables. The analysis of variance resulted in finding several significant main effects and interactions. Male judged the transition of age periods earlier than females for young and middle age adults, and females were perceived as entering the stage of old age at an earlier date than males. A directionality of establishing earlier transition ages by high scorers on the achievement dimension was also noted.", "contents": "Achievement, aggression, and perceived adult age stages. A questionnaire was administered to 173 male and female undergraduates to contrast their perceptions of the transition periods of young, middle age, and old adulthood stages of aging. A comparison of the perceived stages was then made with the personality dimensions of achievement and aggression. A 2 X 2 X 3 analysis of variance was computed for sex of S, sex of the perceived individual, and three adult stages. In addition, coefficients of correlation were obtained for 24 variables. The analysis of variance resulted in finding several significant main effects and interactions. Male judged the transition of age periods earlier than females for young and middle age adults, and females were perceived as entering the stage of old age at an earlier date than males. A directionality of establishing earlier transition ages by high scorers on the achievement dimension was also noted."}
{"id": "PMID:480265", "title": "The effects of age and sex on the factor structure of the Wechsler Memory Scale.", "content": "Memory is one of the central intellectual functions characteristic of human behavior. Increasing age affects memory processes by requiring more time and increased learning trials in such tasks as memory for digits, symbols, and figures, and in the acquisition of new information. Factor analytic studies of the Wechsler Memory Scale (WMS) have been contradictory because of the confounding effects of subject age and sex. Principal component analyses with oblique rotations were performed 16 times on independent and random samples of males and females, and of males and females at each of three developmental age levels: 13-39, 40-59, and 60-88 years. For total male and female samples, a two-factor structure of general retentiveness and memory was found. For both males and females at 13-39 and 40-59 years, the same factor structure was obtained. The two-factor structure did not occur at age 60-88 for males or females. These findings confirm Wechsler's hypothesis as to the structure of the WMS, and Guilford's hypothesis as to the effect of age upon memory.", "contents": "The effects of age and sex on the factor structure of the Wechsler Memory Scale. Memory is one of the central intellectual functions characteristic of human behavior. Increasing age affects memory processes by requiring more time and increased learning trials in such tasks as memory for digits, symbols, and figures, and in the acquisition of new information. Factor analytic studies of the Wechsler Memory Scale (WMS) have been contradictory because of the confounding effects of subject age and sex. Principal component analyses with oblique rotations were performed 16 times on independent and random samples of males and females, and of males and females at each of three developmental age levels: 13-39, 40-59, and 60-88 years. For total male and female samples, a two-factor structure of general retentiveness and memory was found. For both males and females at 13-39 and 40-59 years, the same factor structure was obtained. The two-factor structure did not occur at age 60-88 for males or females. These findings confirm Wechsler's hypothesis as to the structure of the WMS, and Guilford's hypothesis as to the effect of age upon memory."}
{"id": "PMID:480266", "title": "Effects of model warmth on acquisition and performance of modeled behavior in chronic psychotics.", "content": "Hypotheses derived from an earlier study were tested in experimental dyads with 32 adult chronic psychotic state hospital residents of both sexes. Patients either interacted with an adult model who was noncontingently warm and rewarding, or were not exposed initially to a model. In a second phase, the model displayed task responses and novel behaviors incidental to the task, and the patient's subsequent imitation of the model under minimal demand conditions was recorded. In a third phase, the model again displayed the same behaviors, whereupon half the patients in each group were subjected to moderate (explicit verbal request) or high (verbal request plus material rewards) incentives to imitate. Under strong incentive conditions, initial differences between groups on incidental imitation vanished, indicating that a previously positive relationship with a model facilitates imitative performance but not learning.", "contents": "Effects of model warmth on acquisition and performance of modeled behavior in chronic psychotics. Hypotheses derived from an earlier study were tested in experimental dyads with 32 adult chronic psychotic state hospital residents of both sexes. Patients either interacted with an adult model who was noncontingently warm and rewarding, or were not exposed initially to a model. In a second phase, the model displayed task responses and novel behaviors incidental to the task, and the patient's subsequent imitation of the model under minimal demand conditions was recorded. In a third phase, the model again displayed the same behaviors, whereupon half the patients in each group were subjected to moderate (explicit verbal request) or high (verbal request plus material rewards) incentives to imitate. Under strong incentive conditions, initial differences between groups on incidental imitation vanished, indicating that a previously positive relationship with a model facilitates imitative performance but not learning."}
{"id": "PMID:480267", "title": "Attention to stimulus presentation mode as a function of sex.", "content": "This study investigated sex differences in attention to verbal stimuli presented in auditory and visual modes. Ss were 996 male and female students, ages 7 through 17, who observed different forms of a sound motion picture film. One control film contained auditory verbal stimuli only, the other control film contained visual verbal stimuli only, and the experimental film contained simultaneous, conflicting auditory and visual verbal stimuli. Attending behavior was quantified as the number of stimulus specific answers on a paper-and-pencil test administered after presentation of the film. Prior results had indicated that experimental Ss under the age of 12 attended auditory stimuli significantly more often, and those over the age of 13 attended visual stimuli significantly more often. The present experiment concerned sex differences in attending behavior during the crossover period: it was found that females switched their preference for auditory stimuli to visual stimuli earlier (age 11.5) than males (age 13.5). The significant differences between sexes (p less than .05) were attributed to differential conditioning of attitudes, reinforcements, and discriminations regarding the stimulus presentation mode, as well as biological factors.", "contents": "Attention to stimulus presentation mode as a function of sex. This study investigated sex differences in attention to verbal stimuli presented in auditory and visual modes. Ss were 996 male and female students, ages 7 through 17, who observed different forms of a sound motion picture film. One control film contained auditory verbal stimuli only, the other control film contained visual verbal stimuli only, and the experimental film contained simultaneous, conflicting auditory and visual verbal stimuli. Attending behavior was quantified as the number of stimulus specific answers on a paper-and-pencil test administered after presentation of the film. Prior results had indicated that experimental Ss under the age of 12 attended auditory stimuli significantly more often, and those over the age of 13 attended visual stimuli significantly more often. The present experiment concerned sex differences in attending behavior during the crossover period: it was found that females switched their preference for auditory stimuli to visual stimuli earlier (age 11.5) than males (age 13.5). The significant differences between sexes (p less than .05) were attributed to differential conditioning of attitudes, reinforcements, and discriminations regarding the stimulus presentation mode, as well as biological factors."}
{"id": "PMID:480268", "title": "Sex differences and the incidence of left-handedness.", "content": "The higher incidence of left-handedness among males still remains a manifest and interesting phenomenon. For instance, Clark indicated that 7.84% of her male respondents were left-handed compared to 3.91% of the females. A study in South Africa among 882 students (mean age 13 years) yielded similar results: 11.13% of the males were left-handed compared to 7.69% of the females.", "contents": "Sex differences and the incidence of left-handedness. The higher incidence of left-handedness among males still remains a manifest and interesting phenomenon. For instance, Clark indicated that 7.84% of her male respondents were left-handed compared to 3.91% of the females. A study in South Africa among 882 students (mean age 13 years) yielded similar results: 11.13% of the males were left-handed compared to 7.69% of the females."}
{"id": "PMID:480269", "title": "Frustration-instigated behavior and learned helplessness.", "content": "This paper compares Seligman's recent work on learned helplessness with N. R. F. Maier's work on frustration-instigated behavior published some 30 years ago. Some striking similarities are noted between the two approaches, and it is concluded that some of the differences are more apparent than real and that the learned helplessness model could provide an explanation for the \"abnormal fixations\" reported by Maier.", "contents": "Frustration-instigated behavior and learned helplessness. This paper compares Seligman's recent work on learned helplessness with N. R. F. Maier's work on frustration-instigated behavior published some 30 years ago. Some striking similarities are noted between the two approaches, and it is concluded that some of the differences are more apparent than real and that the learned helplessness model could provide an explanation for the \"abnormal fixations\" reported by Maier."}
{"id": "PMID:480270", "title": "Empirically derived personality types among male and female college students.", "content": "Data on the 16 PF obtained from 130 male and female college students were cluster analyzed to produce an empirical personality typology. Two different clustering algorithms were compared. Seven personality types emerged: 1) Well-Adjusted Conservative, 2) Ego-Involved Neurotic, 3) Norm Independent, 4) Socially-Detached Neurotic, 5) Superego Controlled, 6) Self-Assured Experimenter, and 7) Tough-Minded Controlled. Not only did the types differ significantly in personality, but they also were found to be significantly different on nine different measures of interpersonal orientation. Since the types did give intuitive insight into the nature of personality as particular combinations of personality traits and also were different on variables other than those used for the classification, the scientific utility of the typological approach received support.", "contents": "Empirically derived personality types among male and female college students. Data on the 16 PF obtained from 130 male and female college students were cluster analyzed to produce an empirical personality typology. Two different clustering algorithms were compared. Seven personality types emerged: 1) Well-Adjusted Conservative, 2) Ego-Involved Neurotic, 3) Norm Independent, 4) Socially-Detached Neurotic, 5) Superego Controlled, 6) Self-Assured Experimenter, and 7) Tough-Minded Controlled. Not only did the types differ significantly in personality, but they also were found to be significantly different on nine different measures of interpersonal orientation. Since the types did give intuitive insight into the nature of personality as particular combinations of personality traits and also were different on variables other than those used for the classification, the scientific utility of the typological approach received support."}
{"id": "PMID:480271", "title": "Discrimination learning in preschool children as a function of the stimulus and relevant dimension.", "content": "The purpose of the present study was to determine discrimination learning rates as a function of the nature of the stimulus and the relevant dimension. Ss were 160 preschool boys and girls. The design consisted of two stimuli (faces and cups), two constant dimensions (size and form), and two relevant dimensions (form and color when the size dimension was held constant, and size and color when the form dimension was held constant). The errors to criterion scores revealed that for cup stimuli the color dimension was learned more rapidly than size and form, while for face stimuli the dimensions of size and form were learned more rapidly than color.", "contents": "Discrimination learning in preschool children as a function of the stimulus and relevant dimension. The purpose of the present study was to determine discrimination learning rates as a function of the nature of the stimulus and the relevant dimension. Ss were 160 preschool boys and girls. The design consisted of two stimuli (faces and cups), two constant dimensions (size and form), and two relevant dimensions (form and color when the size dimension was held constant, and size and color when the form dimension was held constant). The errors to criterion scores revealed that for cup stimuli the color dimension was learned more rapidly than size and form, while for face stimuli the dimensions of size and form were learned more rapidly than color."}
{"id": "PMID:480272", "title": "Attempted cross-transmission of coccidia between sheep and goats and description of Eimeria ovinoidalis sp. n.", "content": "Attempted infection of 2 young lambs with oocysts of Eimeria christenseni from a goat was unsuccessful. Negative results were obtained also when young kids were fed oocysts of Eimeria ninakohlyakimovae from sheep. There was no difficulty in infecting lambs with the sheep coccidium resembling E. ninakohlyakimovae nor goats with the goat coccidium E. christenseni. Oocysts from the goat measured 38.4 X 26.7 microns, but were easily distinguished from Eimeria ahsata from the sheep by sporocyst size and shape, and from Eimeria ovina by oocyst size. Eimeria ninakohlyakimovae-like oocysts from sheep averaged 23.0 X 18.2 microns and were morphologically indistinguishable from previously reported goat coccidia. Since no cross infections of sheep and goats could be accomplished with oocysts of Eimeria sp. characteristic of one or the other host, I concluded that sheep coccidia previously known as E. ninakohlykimovae are distinct from morphologically similar goat coccidia and therefore constitute a separate species. Since the name E. ninakohlyakimovae was first used for coccidia from the goat, the sheep coccidium is renamed Eimeria ovinoidalis with oocyst structure and endogenous stages similar to those previously described from the sheep.", "contents": "Attempted cross-transmission of coccidia between sheep and goats and description of Eimeria ovinoidalis sp. n. Attempted infection of 2 young lambs with oocysts of Eimeria christenseni from a goat was unsuccessful. Negative results were obtained also when young kids were fed oocysts of Eimeria ninakohlyakimovae from sheep. There was no difficulty in infecting lambs with the sheep coccidium resembling E. ninakohlyakimovae nor goats with the goat coccidium E. christenseni. Oocysts from the goat measured 38.4 X 26.7 microns, but were easily distinguished from Eimeria ahsata from the sheep by sporocyst size and shape, and from Eimeria ovina by oocyst size. Eimeria ninakohlyakimovae-like oocysts from sheep averaged 23.0 X 18.2 microns and were morphologically indistinguishable from previously reported goat coccidia. Since no cross infections of sheep and goats could be accomplished with oocysts of Eimeria sp. characteristic of one or the other host, I concluded that sheep coccidia previously known as E. ninakohlykimovae are distinct from morphologically similar goat coccidia and therefore constitute a separate species. Since the name E. ninakohlyakimovae was first used for coccidia from the goat, the sheep coccidium is renamed Eimeria ovinoidalis with oocyst structure and endogenous stages similar to those previously described from the sheep."}
{"id": "PMID:480273", "title": "Toxoplasma gondii-vertebrate cell interactions. II. The intracellular reproductive phase.", "content": "The reproduction of Toxoplasma gondii RH-strain in vertebrate cells was studied in a controlled-environment culture system. The lag period before reproduction and the doubling time of individual parasites were determined using a least-squares linear regression method of analysis which does not artificially constrain the data. In the majority of cases, the time intercept of the linear regression line was either zero, implying the lack of a lag phase before reproduction, or negative, implying the parasite had completed part of its reproductive cycle before entering the host cell. The mean doubling time of T. gondii is 10.9 h in bovine embryo skeletal muscle cells and 8.3 h in HeLa cells. This difference is not significant at the 5% level. The population doubling times of mouse-derived parasites is best described by a gamma distribution.", "contents": "Toxoplasma gondii-vertebrate cell interactions. II. The intracellular reproductive phase. The reproduction of Toxoplasma gondii RH-strain in vertebrate cells was studied in a controlled-environment culture system. The lag period before reproduction and the doubling time of individual parasites were determined using a least-squares linear regression method of analysis which does not artificially constrain the data. In the majority of cases, the time intercept of the linear regression line was either zero, implying the lack of a lag phase before reproduction, or negative, implying the parasite had completed part of its reproductive cycle before entering the host cell. The mean doubling time of T. gondii is 10.9 h in bovine embryo skeletal muscle cells and 8.3 h in HeLa cells. This difference is not significant at the 5% level. The population doubling times of mouse-derived parasites is best described by a gamma distribution."}
{"id": "PMID:480274", "title": "An experimental investigation of the modifiability of the temporal structure of spontaneous speech.", "content": "This study attempted to test the hypothesis that the temporal structure of spontaneous speech is modifiable by reinforcing and punishing pauses, of a certain duration, in an operant conditioning situation. Pause rate was significantly affected by these contingencies: moreover, rate of change was rapid, indicating a \"prepared\" association between pausing and such contingencies. This study also attempted to test the hypothesis that there is a class of noncognitive pauses in monologue by punishing UPs to determine if UPs can be eliminated without affecting speech content. Although this manipulation did lead to a decline in pause rate, a significant increase in the amount of filled hesitation, particularly in repetition, resulted. This suggests that the overall amount of hesitation is fixed by the cognitive demands of the task but that a speaker is able to adapt to different interactional contexts by varying the category of hesitation used for cognitive planning.", "contents": "An experimental investigation of the modifiability of the temporal structure of spontaneous speech. This study attempted to test the hypothesis that the temporal structure of spontaneous speech is modifiable by reinforcing and punishing pauses, of a certain duration, in an operant conditioning situation. Pause rate was significantly affected by these contingencies: moreover, rate of change was rapid, indicating a \"prepared\" association between pausing and such contingencies. This study also attempted to test the hypothesis that there is a class of noncognitive pauses in monologue by punishing UPs to determine if UPs can be eliminated without affecting speech content. Although this manipulation did lead to a decline in pause rate, a significant increase in the amount of filled hesitation, particularly in repetition, resulted. This suggests that the overall amount of hesitation is fixed by the cognitive demands of the task but that a speaker is able to adapt to different interactional contexts by varying the category of hesitation used for cognitive planning."}
{"id": "PMID:480294", "title": "Urine microscopy and infection in general practice.", "content": "To test the value of urine microscopy 100 consecutive specimens were examined in the surgery and the results correlated with the subsequent laboratory culture reports.An assessment of the degree of pyuria was made by low power microscopy of a thick drop of fresh urine. A second specimen was examined under high power for the presence or absence of motile bacilli. The techniques used are described and quantified.The laboratory report was definitive in 88 of the 100 cultures. All the 33 specimens with 10(5) bacteriuria had some degree of pyuria and in 27 (82 per cent) motile bacilli had been found. In the 50 with no significant bacteriuria no motile bacilli had been seen in 38 (76 per cent).In these 88 specimens a diagnosis made in the surgery based entirely on bacterial microscopy would have been correct in 80 per cent, combined with cytological microscopy in 87 per cent, and with the addition of clinical features in 92 per cent.In the remaining 12 cases the laboratory report was inconclusive and would have made no difference to my conclusions.", "contents": "Urine microscopy and infection in general practice. To test the value of urine microscopy 100 consecutive specimens were examined in the surgery and the results correlated with the subsequent laboratory culture reports.An assessment of the degree of pyuria was made by low power microscopy of a thick drop of fresh urine. A second specimen was examined under high power for the presence or absence of motile bacilli. The techniques used are described and quantified.The laboratory report was definitive in 88 of the 100 cultures. All the 33 specimens with 10(5) bacteriuria had some degree of pyuria and in 27 (82 per cent) motile bacilli had been found. In the 50 with no significant bacteriuria no motile bacilli had been seen in 38 (76 per cent).In these 88 specimens a diagnosis made in the surgery based entirely on bacterial microscopy would have been correct in 80 per cent, combined with cytological microscopy in 87 per cent, and with the addition of clinical features in 92 per cent.In the remaining 12 cases the laboratory report was inconclusive and would have made no difference to my conclusions."}
{"id": "PMID:480297", "title": "Intuition and problem solving.", "content": "General practitioners use the hypothetico-deductive method of scientific reasoning to solve problems. In the first few minutes of their consultations physicians form initial hypotheses about their patients' problems. This process has childlike, imaginative qualities based on intuition. It is often outside consciousness and probably based on pattern recognition. It has been neglected from study and analysis because of these ;mystical' qualities. Yet it is the key to fast and efficient problem solving. If the process could be understood, its efficiency would be improved. This paper is an attempt to explore the nature of general practitioners' intuition.", "contents": "Intuition and problem solving. General practitioners use the hypothetico-deductive method of scientific reasoning to solve problems. In the first few minutes of their consultations physicians form initial hypotheses about their patients' problems. This process has childlike, imaginative qualities based on intuition. It is often outside consciousness and probably based on pattern recognition. It has been neglected from study and analysis because of these ;mystical' qualities. Yet it is the key to fast and efficient problem solving. If the process could be understood, its efficiency would be improved. This paper is an attempt to explore the nature of general practitioners' intuition."}
{"id": "PMID:480298", "title": "The doctor/patient relationship and its effect upon outcome.", "content": "The study of 299 chronically ill patients examined the doctor/patient relationship by asking two questions: first, what factors affect the quality of the relationship and secondly, does the doctor/patient relationship affect outcome for the patient? The doctor/patient relationship was measured by indicators of the doctor's awareness of the patient's problems.The following factors were found to be positively associated with the doctor's awareness: a small number of patient problems, a large number of recent visits, and the patient, rather than the doctor, initiating the consultation. The patient's age and education, the completeness of family care, and duration of care were not found to influence awareness. After eliminating the effect of confounding variables, the relationship between the doctor's awareness and the patient's recovery was maintained for some groups of patients. Awareness did not significantly affect the patient's satisfaction. We list some practical recommendations to aid doctors in increasing their knowledge of their patients.", "contents": "The doctor/patient relationship and its effect upon outcome. The study of 299 chronically ill patients examined the doctor/patient relationship by asking two questions: first, what factors affect the quality of the relationship and secondly, does the doctor/patient relationship affect outcome for the patient? The doctor/patient relationship was measured by indicators of the doctor's awareness of the patient's problems.The following factors were found to be positively associated with the doctor's awareness: a small number of patient problems, a large number of recent visits, and the patient, rather than the doctor, initiating the consultation. The patient's age and education, the completeness of family care, and duration of care were not found to influence awareness. After eliminating the effect of confounding variables, the relationship between the doctor's awareness and the patient's recovery was maintained for some groups of patients. Awareness did not significantly affect the patient's satisfaction. We list some practical recommendations to aid doctors in increasing their knowledge of their patients."}
{"id": "PMID:480299", "title": "An evaluation of the A4 folder system in general practice.", "content": "All 63 general practices known to have introduced the A4 folder system were asked to complete questionnaires comparing A4 records with the traditional medical records envelope system. Of the 77 per cent who replied, an overwhelming majority favoured the A4 system and felt that it helped them to obtain higher standards of care for patients.", "contents": "An evaluation of the A4 folder system in general practice. All 63 general practices known to have introduced the A4 folder system were asked to complete questionnaires comparing A4 records with the traditional medical records envelope system. Of the 77 per cent who replied, an overwhelming majority favoured the A4 system and felt that it helped them to obtain higher standards of care for patients."}
{"id": "PMID:480300", "title": "Some difficulties in comparing morbidity between countries.", "content": "During a two-month period in general practice we compared morbidity records from the teaching practices at Southampton, UK, and Nijmegen, The Netherlands. Although the commonest conditions - emotional disorders, upper respiratory tract infection, and musculo-skeletal disorders - were equally prevalent, obesity was five times as prevalent and hypertension and urinary tract infection were twice as prevalent in Nijmegen as in Southampton. The Dutch doctors were far more ready to prescribe oral contraception to women over 50 years old. We met many difficulties in what had appeared to be a simple project and our results may reflect important differences about doctors' attitudes to care as well as differences in morbidity.", "contents": "Some difficulties in comparing morbidity between countries. During a two-month period in general practice we compared morbidity records from the teaching practices at Southampton, UK, and Nijmegen, The Netherlands. Although the commonest conditions - emotional disorders, upper respiratory tract infection, and musculo-skeletal disorders - were equally prevalent, obesity was five times as prevalent and hypertension and urinary tract infection were twice as prevalent in Nijmegen as in Southampton. The Dutch doctors were far more ready to prescribe oral contraception to women over 50 years old. We met many difficulties in what had appeared to be a simple project and our results may reflect important differences about doctors' attitudes to care as well as differences in morbidity."}
{"id": "PMID:480302", "title": "The distribution of carnitine and acetylcarnitine in the rabbit epididymis and the carnitine content of rabbit spermatozoa during maturation.", "content": "The highest levels of carnitine and acetylcarnitine were found in the cauda, and spermatozoa from the proximal cauda contained significantly greater amounts of carnitine than those removed from the corpus or caput epididymidis. Acetylcarnitine levels (as a % of the total carnitine pool) were greater in all regions of the rabbit epididymis than has been reported in other species. It is suggested that the accumulation of carnitine is involved in sperm maturation.", "contents": "The distribution of carnitine and acetylcarnitine in the rabbit epididymis and the carnitine content of rabbit spermatozoa during maturation. The highest levels of carnitine and acetylcarnitine were found in the cauda, and spermatozoa from the proximal cauda contained significantly greater amounts of carnitine than those removed from the corpus or caput epididymidis. Acetylcarnitine levels (as a % of the total carnitine pool) were greater in all regions of the rabbit epididymis than has been reported in other species. It is suggested that the accumulation of carnitine is involved in sperm maturation."}
{"id": "PMID:480303", "title": "Plasma relaxin immunoactivity in the pig at parturition and during nuzzling and suckling.", "content": "One sow bled at 30--60-min intervals for 48 h at 5 and 4 days before parturition had mean +/- s.e.m. relaxin levels of 5.0 +/- 0.48 ng/ml and 5.5 +/- 0.44 ng/ml for each 24-h period respectively. This sow and another were bled at frequent intervals during parturition; both showed considerable fluctuations in their relaxin levels but no consistent peaks in relation to each birth. Mean levels during parturition were 10.7 +/- 0.46 ng/ml and 13.4 +/- 0.81 ng/ml respectively, both significantly higher than the levels at 4 and 5 days before birth. Relaxin levels in two lactating sows rose acutely during nursing, showing a 3-fold rise in one animal and an 8-fold rise in the other. Results from a third sow during an extended period of nuzzling and sucking by the piglets showed multiple peaks of relaxin immunoactivity associated with each nuzzling/sucking stimulus.", "contents": "Plasma relaxin immunoactivity in the pig at parturition and during nuzzling and suckling. One sow bled at 30--60-min intervals for 48 h at 5 and 4 days before parturition had mean +/- s.e.m. relaxin levels of 5.0 +/- 0.48 ng/ml and 5.5 +/- 0.44 ng/ml for each 24-h period respectively. This sow and another were bled at frequent intervals during parturition; both showed considerable fluctuations in their relaxin levels but no consistent peaks in relation to each birth. Mean levels during parturition were 10.7 +/- 0.46 ng/ml and 13.4 +/- 0.81 ng/ml respectively, both significantly higher than the levels at 4 and 5 days before birth. Relaxin levels in two lactating sows rose acutely during nursing, showing a 3-fold rise in one animal and an 8-fold rise in the other. Results from a third sow during an extended period of nuzzling and sucking by the piglets showed multiple peaks of relaxin immunoactivity associated with each nuzzling/sucking stimulus."}
{"id": "PMID:480304", "title": "The effect of pregnancy on the interval between one oestrus and the next in the tammar wallaby, Macropus eugenii.", "content": "In females mated to vasectomized males the interval between oestrous periods was 30.6 +/- 1.17 (s.d.) days but when mated to intact males and pregnancy intervened, it was 29. +/- 1.26 days (P less than 0.0025). After removal of pouch young, females carrying diapausing blastocysts gave birth 26.2 days later and came into oestrus 26.4 +/- 0.57 (s.d.) days later. When post-partum fertilization was prevented, removal of pouch young was followed by oestrus 30.4 +/- 0.99 days later (P less than 0.0005). These results indicate an influence of the conceptus upon the oestrous cycle of Macropus eugenii.", "contents": "The effect of pregnancy on the interval between one oestrus and the next in the tammar wallaby, Macropus eugenii. In females mated to vasectomized males the interval between oestrous periods was 30.6 +/- 1.17 (s.d.) days but when mated to intact males and pregnancy intervened, it was 29. +/- 1.26 days (P less than 0.0025). After removal of pouch young, females carrying diapausing blastocysts gave birth 26.2 days later and came into oestrus 26.4 +/- 0.57 (s.d.) days later. When post-partum fertilization was prevented, removal of pouch young was followed by oestrus 30.4 +/- 0.99 days later (P less than 0.0005). These results indicate an influence of the conceptus upon the oestrous cycle of Macropus eugenii."}
{"id": "PMID:480305", "title": "Synthesis of steroids by echidna (Tachyglossus aculeatus) testis and the effects of gonadotrophin administration.", "content": "After administration of hCG and PMSG to male echidnas (in non-breeding state) the testis to body weight ratio increased 3-fold and the diameter of the seminiferous tubules doubled, but spermatogenesis was not induced. The major conversion product of testicular homogenate with [4-14C]progesterone as substrate was 17 alpha-hydroxyprogesterone (4% in untreated and 29% in treated echidnas). Testosterone (5%), androstenedione (16%) and 17 alpha,20 beta-dihydroxypregn-4-en-3-one (7%) were also obtained in gonadotrophin-treated animals. In untreated animals these steroids were present in minor amounts (less than 1%).", "contents": "Synthesis of steroids by echidna (Tachyglossus aculeatus) testis and the effects of gonadotrophin administration. After administration of hCG and PMSG to male echidnas (in non-breeding state) the testis to body weight ratio increased 3-fold and the diameter of the seminiferous tubules doubled, but spermatogenesis was not induced. The major conversion product of testicular homogenate with [4-14C]progesterone as substrate was 17 alpha-hydroxyprogesterone (4% in untreated and 29% in treated echidnas). Testosterone (5%), androstenedione (16%) and 17 alpha,20 beta-dihydroxypregn-4-en-3-one (7%) were also obtained in gonadotrophin-treated animals. In untreated animals these steroids were present in minor amounts (less than 1%)."}
{"id": "PMID:480306", "title": "Time requirement for capacitation of boar spermatozoa assessed by their ability to penetrate the zona-free hamster egg.", "content": "Boar spermatozoa were preincubated for various times in the isolated uterus and oviduct from a maturing gilt and used to inseminate zona-free hamster eggs. The proportions of eggs penetrated and activated were increased, and the interval between insemination and sperm penetration was shortened when the spermatozoa were preincubated for 4--5.5 h instead of 2--.5 h. Overall penetration rates were higher and sperm penetration occurred about 1 h earlier when the eggs were inseminated with spermatozoa preincubated in the uterus than in the oviduct. It is concluded that the change in ability of boar spermatozoa to penetrate zona-free hamster eggs is due to capcitation which requires 4--4.5 h and 5--5.5 h of preincubation in the isolated uterus and oviduct, respectively.", "contents": "Time requirement for capacitation of boar spermatozoa assessed by their ability to penetrate the zona-free hamster egg. Boar spermatozoa were preincubated for various times in the isolated uterus and oviduct from a maturing gilt and used to inseminate zona-free hamster eggs. The proportions of eggs penetrated and activated were increased, and the interval between insemination and sperm penetration was shortened when the spermatozoa were preincubated for 4--5.5 h instead of 2--.5 h. Overall penetration rates were higher and sperm penetration occurred about 1 h earlier when the eggs were inseminated with spermatozoa preincubated in the uterus than in the oviduct. It is concluded that the change in ability of boar spermatozoa to penetrate zona-free hamster eggs is due to capcitation which requires 4--4.5 h and 5--5.5 h of preincubation in the isolated uterus and oviduct, respectively."}
{"id": "PMID:480307", "title": "Mechanism of liquefaction of the human ejaculate. I. Changes of the ejaculate proteins.", "content": "The spontaneous liquefaction of the human ejaculate in vitro was studied by macroscopic inspection, disc electrophoresis of the ejaculate proteins, free amino acid and nitrogen determinations and by electron microscopy. Liquefaction occurred in three phases. Phase 1 was characterized by the macroscopic solubilization of the gelatinous material and by disappearance of its granular ultrastructure. Zn++, Hg++ and Cu++ completely inhibited the first step of the liquefraction, which could be reversed by Na2EDTA. The factor(s) involved is most probably a proteolytic enzyme(s) which is tightly incorporated into the gelatinous material. In Phase 2 of liquefaction solubilized proteins were degraded to peptides and in Phase 3 these peptides were degraded to amino acids. Factor(s) responsible for these two steps of liquefaction could be removed by washing the gelatinous material.", "contents": "Mechanism of liquefaction of the human ejaculate. I. Changes of the ejaculate proteins. The spontaneous liquefaction of the human ejaculate in vitro was studied by macroscopic inspection, disc electrophoresis of the ejaculate proteins, free amino acid and nitrogen determinations and by electron microscopy. Liquefaction occurred in three phases. Phase 1 was characterized by the macroscopic solubilization of the gelatinous material and by disappearance of its granular ultrastructure. Zn++, Hg++ and Cu++ completely inhibited the first step of the liquefraction, which could be reversed by Na2EDTA. The factor(s) involved is most probably a proteolytic enzyme(s) which is tightly incorporated into the gelatinous material. In Phase 2 of liquefaction solubilized proteins were degraded to peptides and in Phase 3 these peptides were degraded to amino acids. Factor(s) responsible for these two steps of liquefaction could be removed by washing the gelatinous material."}
{"id": "PMID:480308", "title": "Catecholamine requirement for hamster sperm motility in vitro.", "content": "Homogenates of hamster and bovine glands contain a \"sperm motility factor\" (SMF) that stimulates the motility of hamster epididymal spermatozoa in vitro. The potency of these adrenal preparations was severely attenuated after gel filtration on a Sephadex G-10 column. This loss of activity was ascribed to the retardation and separation of co-factors for SMF which appeared to be catecholamines. The sperm motility-stimulating activity of the SMF-containing fractions was fully restored by addition of either the 'retarded' fractions or catecholamines (epinephrine or norepinephrine). Neither the catecholamines nor the 'retarded' fractions were able to sustain vigorous sperm motility in the absence of the SMF-containing fractions. The potentiating action of catecholamines on SMF was mimicked by the adrenergic agonists isoproterenol and phenylephrine and inhibited by the alpha-adrenergic antagonist phentolamine, but not by the beta-adrenergic antagonist propranolol. Our results indicate that one or more catecholamines are essential co-factors of SMF and demonstrate that hamster spermatozoa require catecholamines for their motility in vitro.", "contents": "Catecholamine requirement for hamster sperm motility in vitro. Homogenates of hamster and bovine glands contain a \"sperm motility factor\" (SMF) that stimulates the motility of hamster epididymal spermatozoa in vitro. The potency of these adrenal preparations was severely attenuated after gel filtration on a Sephadex G-10 column. This loss of activity was ascribed to the retardation and separation of co-factors for SMF which appeared to be catecholamines. The sperm motility-stimulating activity of the SMF-containing fractions was fully restored by addition of either the 'retarded' fractions or catecholamines (epinephrine or norepinephrine). Neither the catecholamines nor the 'retarded' fractions were able to sustain vigorous sperm motility in the absence of the SMF-containing fractions. The potentiating action of catecholamines on SMF was mimicked by the adrenergic agonists isoproterenol and phenylephrine and inhibited by the alpha-adrenergic antagonist phentolamine, but not by the beta-adrenergic antagonist propranolol. Our results indicate that one or more catecholamines are essential co-factors of SMF and demonstrate that hamster spermatozoa require catecholamines for their motility in vitro."}
{"id": "PMID:480309", "title": "Serum gonadotrophin levels in prepubertally castrated male sheep treated for long periods with propionated testosterone, dihydrotestosterone, 19-hydroxytestosterone or oestradiol.", "content": "At different times of the year, groups of wethers were treated with 20 mg testosterone, dihydrotestosterone or 19-hydroxytestosterone propionates/day or 2 mg oestradiol dipropionate/day, or the oil vehicle, for 6 weeks after a 2-week control period. LH and FSH values were determined by radioimmunoassay of serum samples collected at regular intervals. Oestradiol and dihydrotestosterone reduced LH and FSH concentrations whereas 19-hydroxytestosterone and testosterone had no effect.", "contents": "Serum gonadotrophin levels in prepubertally castrated male sheep treated for long periods with propionated testosterone, dihydrotestosterone, 19-hydroxytestosterone or oestradiol. At different times of the year, groups of wethers were treated with 20 mg testosterone, dihydrotestosterone or 19-hydroxytestosterone propionates/day or 2 mg oestradiol dipropionate/day, or the oil vehicle, for 6 weeks after a 2-week control period. LH and FSH values were determined by radioimmunoassay of serum samples collected at regular intervals. Oestradiol and dihydrotestosterone reduced LH and FSH concentrations whereas 19-hydroxytestosterone and testosterone had no effect."}
{"id": "PMID:480310", "title": "The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa.", "content": "Ejaculated bull spermatozoa were exposed to various concentrations of ouabain to ascertain the effect on membrane potential, intracellular concentrations of sodium and potassium and motility. Membrane potenital, measured by electrophysiological methods, decreased. Intracellular potassium decreased and intracellular sodium increased. Progressive motility decreased. In addition, the motility change produced by ouabain was identified as a decrease both in beat frequency and in wave amplitude.", "contents": "The effect of ouabain on membrane potential and flagellar wave in ejaculated bull spermatozoa. Ejaculated bull spermatozoa were exposed to various concentrations of ouabain to ascertain the effect on membrane potential, intracellular concentrations of sodium and potassium and motility. Membrane potenital, measured by electrophysiological methods, decreased. Intracellular potassium decreased and intracellular sodium increased. Progressive motility decreased. In addition, the motility change produced by ouabain was identified as a decrease both in beat frequency and in wave amplitude."}
{"id": "PMID:480311", "title": "Affinity of uterine luminal proteins for rat blastocysts.", "content": "The binding of 125I-labelled rat uterine luminal proteins from Day-5 pregnant rats showed higher binding affinity to blastocysts than did the binding of proteins in uterine fluid from pro-oestrous rats (Day 0), rat serum albumin (RSA) or bovine serum albumin (BSA). Apparently little uptake of proteins into cells by phagocytosis or entry into the blastocoelic cavity occurred since similar results were obtained in the presence of sodium azide or cytochalasin B. Autoradiographic studies showed that the proteins were localized on the outer surface of the blastocyst. The binding was Ca2+-dependent. Denaturation of Day-5 uterine proteins at 80 degrees C reduced the counts to the values obtained with undenatured RSA and Day-0 fluids; this residual binding was considered as non-specific. The binding of labelled Day-5 uterine proteins was substantially reduced in the presence of unlabelled Day-5 proteins but to a lesser extent in the presence of RSA or rat serum. The dissociation of the bound labelled Day-5 uterine proteins occurred most rapidly in the presence of unlabelled Day-5 proteins. However, dissociation occurred within 2 h in the presence of other macromolecules, suggesting that the binding was not strong.", "contents": "Affinity of uterine luminal proteins for rat blastocysts. The binding of 125I-labelled rat uterine luminal proteins from Day-5 pregnant rats showed higher binding affinity to blastocysts than did the binding of proteins in uterine fluid from pro-oestrous rats (Day 0), rat serum albumin (RSA) or bovine serum albumin (BSA). Apparently little uptake of proteins into cells by phagocytosis or entry into the blastocoelic cavity occurred since similar results were obtained in the presence of sodium azide or cytochalasin B. Autoradiographic studies showed that the proteins were localized on the outer surface of the blastocyst. The binding was Ca2+-dependent. Denaturation of Day-5 uterine proteins at 80 degrees C reduced the counts to the values obtained with undenatured RSA and Day-0 fluids; this residual binding was considered as non-specific. The binding of labelled Day-5 uterine proteins was substantially reduced in the presence of unlabelled Day-5 proteins but to a lesser extent in the presence of RSA or rat serum. The dissociation of the bound labelled Day-5 uterine proteins occurred most rapidly in the presence of unlabelled Day-5 proteins. However, dissociation occurred within 2 h in the presence of other macromolecules, suggesting that the binding was not strong."}
{"id": "PMID:480312", "title": "Altered response of cervical and vaginal epithelia to oestradiol benzoate in ewes after prolonged exposure to oestrogenic pasture.", "content": "Ovariectomized ewes, 10 clover disease infertility and 10 controls, were injected daily for 3 days with 25 micrograms oestradiol benzoate. At the end of this period, the Spinnbarkeit of cervical mucus and the keratinization of the vaginal epithelium were less in the infertile ewes. It is suggested that the infertility in  clover-affected ewes is due to an inability of target organs to give a continued response to the 'priming' action of oestrogen.", "contents": "Altered response of cervical and vaginal epithelia to oestradiol benzoate in ewes after prolonged exposure to oestrogenic pasture. Ovariectomized ewes, 10 clover disease infertility and 10 controls, were injected daily for 3 days with 25 micrograms oestradiol benzoate. At the end of this period, the Spinnbarkeit of cervical mucus and the keratinization of the vaginal epithelium were less in the infertile ewes. It is suggested that the infertility in  clover-affected ewes is due to an inability of target organs to give a continued response to the 'priming' action of oestrogen."}
{"id": "PMID:480313", "title": "The movement of pyruvate, lactate and lactate dehydrogenase into rabbit oviductal fluid.", "content": "Pyruvate, lactate and lactate dehydrogenase appeared linearly in 2 ml 0.9% NaCl recirculated through the rabbit oviduct for 4 h in vivo. In oviducts from rabbits injected 3 days previously with 100 i.u. hCG, the rate of appearance of all three constituents was considerably reduced. It is considered unlikely that the lactate dehydrogenase secreted brings about the interconversion of pyruvate and lactate in the oviduct lumen.", "contents": "The movement of pyruvate, lactate and lactate dehydrogenase into rabbit oviductal fluid. Pyruvate, lactate and lactate dehydrogenase appeared linearly in 2 ml 0.9% NaCl recirculated through the rabbit oviduct for 4 h in vivo. In oviducts from rabbits injected 3 days previously with 100 i.u. hCG, the rate of appearance of all three constituents was considerably reduced. It is considered unlikely that the lactate dehydrogenase secreted brings about the interconversion of pyruvate and lactate in the oviduct lumen."}
{"id": "PMID:480314", "title": "The movement of amino acids into rabbit oviductal fluid.", "content": "Twenty-three amino acids appeared linearly in oviduct fluid to reach an average concentration 27% of that in the plasma, with glycine and alanine present in greatest amount. In contrast, the concentration of urea in the fluid approached its plasma concentration after 1 h, and remained at this level for the duration of the experiment. The oviduct was unable to bring the overall uphill transport of amino acids from plasma to oviduct lumen. The amounts of amino acids appearing in the oviduct lumen of rabbits injected 3 days previously with hCG were mostly similar to those of the controls, but the movements of 6 neutral amino acids appeared to be enhanced relative to their plasma concentrations following ovulation.", "contents": "The movement of amino acids into rabbit oviductal fluid. Twenty-three amino acids appeared linearly in oviduct fluid to reach an average concentration 27% of that in the plasma, with glycine and alanine present in greatest amount. In contrast, the concentration of urea in the fluid approached its plasma concentration after 1 h, and remained at this level for the duration of the experiment. The oviduct was unable to bring the overall uphill transport of amino acids from plasma to oviduct lumen. The amounts of amino acids appearing in the oviduct lumen of rabbits injected 3 days previously with hCG were mostly similar to those of the controls, but the movements of 6 neutral amino acids appeared to be enhanced relative to their plasma concentrations following ovulation."}
{"id": "PMID:480315", "title": "The effect of sympathomimetic drugs on contractility of the vas deferens in vitro and in vivo.", "content": "The sympathomimetic drugs noradrenaline, methoxamine, tyramine and norephedrine caused rhythmic contractions in isolated human vasa deferentia. Provided the drug was not washed out, these contractions lasted for the entire duration of the experiment (4-6 h). These contractions were mediated via alpha-adrenoreceptors. Intravenous administration of methoxamine or oxymetazolene to rats or guinea-pigs produced contractions of the vas deferens in vivo in some experiments but was accompanied by severe cardiovascular side effects. A local method of application was developed, using mixtures of tyramine with Silastic prepared as collars specially designed to fit round the vas deferens. Acute and chronic insertion of these slow-releasing devices around the vas deferens of rats produced rhythmic contractions of the vas deferens without any serious side effects.", "contents": "The effect of sympathomimetic drugs on contractility of the vas deferens in vitro and in vivo. The sympathomimetic drugs noradrenaline, methoxamine, tyramine and norephedrine caused rhythmic contractions in isolated human vasa deferentia. Provided the drug was not washed out, these contractions lasted for the entire duration of the experiment (4-6 h). These contractions were mediated via alpha-adrenoreceptors. Intravenous administration of methoxamine or oxymetazolene to rats or guinea-pigs produced contractions of the vas deferens in vivo in some experiments but was accompanied by severe cardiovascular side effects. A local method of application was developed, using mixtures of tyramine with Silastic prepared as collars specially designed to fit round the vas deferens. Acute and chronic insertion of these slow-releasing devices around the vas deferens of rats produced rhythmic contractions of the vas deferens without any serious side effects."}
{"id": "PMID:480316", "title": "Antifertility effect of sympathomimetic drugs on male rats when applied locally to the vas deferens.", "content": "Local application of collars containing 25% methoxamine, 50% or 75% tyramine or 50% norephedrine to both vasa deferentia of rats caused a reduction in fertility but not in their ability to mate. A gradual return to fertility was seen in those animals which received the lower dose of tyramine or norephedrine, while the other treatments caused a permanent reduction in fertility. The cause of sterility was production of azoospermic ejaculates resulting from either a block in sperm transport in the vas deferens or from a deficiency in the ejaculatory mechanism. Only methoxamine caused a mechanical obstruction of the vas deferens, but it is possible that the other drugs caused a sustained spasm. An emission defect could have been due to transmitter depletion, receptor-specific desensitization or presynaptic alpha-adrenoreceptor-mediated inhibition. Spermatozoa from the cauda epididymidis were immotile following the treatments.", "contents": "Antifertility effect of sympathomimetic drugs on male rats when applied locally to the vas deferens. Local application of collars containing 25% methoxamine, 50% or 75% tyramine or 50% norephedrine to both vasa deferentia of rats caused a reduction in fertility but not in their ability to mate. A gradual return to fertility was seen in those animals which received the lower dose of tyramine or norephedrine, while the other treatments caused a permanent reduction in fertility. The cause of sterility was production of azoospermic ejaculates resulting from either a block in sperm transport in the vas deferens or from a deficiency in the ejaculatory mechanism. Only methoxamine caused a mechanical obstruction of the vas deferens, but it is possible that the other drugs caused a sustained spasm. An emission defect could have been due to transmitter depletion, receptor-specific desensitization or presynaptic alpha-adrenoreceptor-mediated inhibition. Spermatozoa from the cauda epididymidis were immotile following the treatments."}
{"id": "PMID:480317", "title": "Induction of meiosis in fetal mouse testis in vitro by rete testis tissue from pubertal mice and bulls.", "content": "To test whether a meiosis-inducing substance (MIS) is responsible for the induction of meiosis in the testis at puberty, pubertal mouse rete testis was grown with (1) fetal undifferentiated mouse testis attached to the other side of a filter and (2) the used medium obtained from culture of the rete testis of a pubertal bull for 2 days. In both systems meiosis was induced in the fetal testis showing that MIS is not species specific. No meiosis-preventing effect was seen and it is concluded that meiosis in the testis is triggered at puberty as a result of the activity of the MIS concomitant with decreased activity of the meiosis-preventing substance.", "contents": "Induction of meiosis in fetal mouse testis in vitro by rete testis tissue from pubertal mice and bulls. To test whether a meiosis-inducing substance (MIS) is responsible for the induction of meiosis in the testis at puberty, pubertal mouse rete testis was grown with (1) fetal undifferentiated mouse testis attached to the other side of a filter and (2) the used medium obtained from culture of the rete testis of a pubertal bull for 2 days. In both systems meiosis was induced in the fetal testis showing that MIS is not species specific. No meiosis-preventing effect was seen and it is concluded that meiosis in the testis is triggered at puberty as a result of the activity of the MIS concomitant with decreased activity of the meiosis-preventing substance."}
{"id": "PMID:480318", "title": "Carnitine, acetylcarnitine and the activity of carnitine acyltransferases in seminal plasma and spermatozoa of men, rams and rats.", "content": "The concentration of total carnitine (i.e. carnitine plus acetylcarnitine) was measured in seminal plasma and spermatozoa of men and rams. In ram semen, there was a close correlation between the concentration of spermatozoa and that of total carnitine in the seminal plasma, indicating that the epididymal secretion was the sole source of seminal carnitine. The percentage of total carnitine present as acetylcarnitine was 40% in seminal plasma and 70-80% in spermatozoa. The acetylation state of carnitine in seminal plasma was apparently not influenced by the metabolic activity of spermatozoa in ejaculated ram semen as no change was found in the plasma concentration of carnitine or acetylcarnitine up to 45 min after ejaculation. In spermatozoa, the activity of carnitine acetyltransferase (EC 2.3.1.7) was approximately equivalent to that of carnitine palmitoyltransferase (EC 2.3.1.21); and the activity of these enzymes was similar in ram and human spermatozoa but greater in rat spermatozoa. It is concluded that there is no correlation between the content of either total carnitine or the carnitine acyltransferases and the respiratory capacity of spermatozoa.", "contents": "Carnitine, acetylcarnitine and the activity of carnitine acyltransferases in seminal plasma and spermatozoa of men, rams and rats. The concentration of total carnitine (i.e. carnitine plus acetylcarnitine) was measured in seminal plasma and spermatozoa of men and rams. In ram semen, there was a close correlation between the concentration of spermatozoa and that of total carnitine in the seminal plasma, indicating that the epididymal secretion was the sole source of seminal carnitine. The percentage of total carnitine present as acetylcarnitine was 40% in seminal plasma and 70-80% in spermatozoa. The acetylation state of carnitine in seminal plasma was apparently not influenced by the metabolic activity of spermatozoa in ejaculated ram semen as no change was found in the plasma concentration of carnitine or acetylcarnitine up to 45 min after ejaculation. In spermatozoa, the activity of carnitine acetyltransferase (EC 2.3.1.7) was approximately equivalent to that of carnitine palmitoyltransferase (EC 2.3.1.21); and the activity of these enzymes was similar in ram and human spermatozoa but greater in rat spermatozoa. It is concluded that there is no correlation between the content of either total carnitine or the carnitine acyltransferases and the respiratory capacity of spermatozoa."}
{"id": "PMID:480319", "title": "A high incidence of abdominal pregnancy in the Djungarian hamster (Phodopus sungorus).", "content": "Eight animals with abdominal fetuses were found in a relatively small colony of Djungarian hamsters. All the fetuses were macerated. Some had remains of an amniotic sac with an umbilical attachment to a placenta adherent to the peritoneum, but others were without placental remains. Gross and histological examinations failed to reveal any ruptures of the uterus or oviducts. It was not possible to decide whether the abdominal pregnancies were primary or secondary.", "contents": "A high incidence of abdominal pregnancy in the Djungarian hamster (Phodopus sungorus). Eight animals with abdominal fetuses were found in a relatively small colony of Djungarian hamsters. All the fetuses were macerated. Some had remains of an amniotic sac with an umbilical attachment to a placenta adherent to the peritoneum, but others were without placental remains. Gross and histological examinations failed to reveal any ruptures of the uterus or oviducts. It was not possible to decide whether the abdominal pregnancies were primary or secondary."}
{"id": "PMID:480320", "title": "Effect of exogenous gonadotrophin (PMSG) on the antral follicle population in the sheep.", "content": "Follicles were obtained from the ovaries of four groups of 15 ewes. Ewes in the control group were ovariectomized on the 12th day of the oestrous cycle. The other ewes were all given PMSG on the 12th day of the cycle; some were ovariectomized 24 or 40 h later, the others were given prostaglandin followed by hCG and were ovariectomized 6 or 12 h after the hCG injection. All follicles greater than 2 mm in diameter were measured and examined macroscopically for signs of atresia. Some were subjected to detailed morphological examination, the pattern of steroid secretion was determined in others. All the evidence from these three approaches suggested that, in vivo, reversal of the atretic process ('rescue') plays no part in the increase in the number of follicles observed following administration of PMSG.", "contents": "Effect of exogenous gonadotrophin (PMSG) on the antral follicle population in the sheep. Follicles were obtained from the ovaries of four groups of 15 ewes. Ewes in the control group were ovariectomized on the 12th day of the oestrous cycle. The other ewes were all given PMSG on the 12th day of the cycle; some were ovariectomized 24 or 40 h later, the others were given prostaglandin followed by hCG and were ovariectomized 6 or 12 h after the hCG injection. All follicles greater than 2 mm in diameter were measured and examined macroscopically for signs of atresia. Some were subjected to detailed morphological examination, the pattern of steroid secretion was determined in others. All the evidence from these three approaches suggested that, in vivo, reversal of the atretic process ('rescue') plays no part in the increase in the number of follicles observed following administration of PMSG."}
{"id": "PMID:480321", "title": "Maternal nest building by pseudopregnant mice.", "content": "Pseudopregnant mice displayed maternal nest-building behaviour. Their nests were smaller but qualitatively identical to those constructed by pregnant animals.", "contents": "Maternal nest building by pseudopregnant mice. Pseudopregnant mice displayed maternal nest-building behaviour. Their nests were smaller but qualitatively identical to those constructed by pregnant animals."}
{"id": "PMID:480322", "title": "Embryo survival in heifers after transfer of an egg to the uterine horn contralateral to the corpus luteum and the effect of treatments with progesterone or hCG on pregnancy rates.", "content": "Day 7 eggs (oestrus = Day 0) were surgically transferred singly to the uterine horn contralateral to the CL and heifers were either slaughtered on Day 24-26 (Group 1) or palpated per rectum on Day 42 (Group 2) to determine the presence of a developing conceptus and a maintained corpus luteum. There was a fall in pregnancy rate of the control heifers in Groups 1a (6/10) and 2a (2/10) (P = 0.085). Pregnancy rate was unaffected by progesterone treatments from Day 13 to the day before slaughter in Group 1b (8/10), or to Day 23 in Group 2b (2/10). Two of the conceptuses recovered in Group 1b were developing in the presence of regressed corpora lutea. There was, however, an increase in pregnancy rate in Group 2d (6/10) compared with Group 2a (2/10) when heifers were treated with hCG from Days 13 to 35 (P = 0.085). The results indicated that embryonic death is occurring after Day 24 and suggest that a change in the nature of the embryonic signal may be occurring at this time.", "contents": "Embryo survival in heifers after transfer of an egg to the uterine horn contralateral to the corpus luteum and the effect of treatments with progesterone or hCG on pregnancy rates. Day 7 eggs (oestrus = Day 0) were surgically transferred singly to the uterine horn contralateral to the CL and heifers were either slaughtered on Day 24-26 (Group 1) or palpated per rectum on Day 42 (Group 2) to determine the presence of a developing conceptus and a maintained corpus luteum. There was a fall in pregnancy rate of the control heifers in Groups 1a (6/10) and 2a (2/10) (P = 0.085). Pregnancy rate was unaffected by progesterone treatments from Day 13 to the day before slaughter in Group 1b (8/10), or to Day 23 in Group 2b (2/10). Two of the conceptuses recovered in Group 1b were developing in the presence of regressed corpora lutea. There was, however, an increase in pregnancy rate in Group 2d (6/10) compared with Group 2a (2/10) when heifers were treated with hCG from Days 13 to 35 (P = 0.085). The results indicated that embryonic death is occurring after Day 24 and suggest that a change in the nature of the embryonic signal may be occurring at this time."}
{"id": "PMID:480323", "title": "Chronic guanethidine treatment of female rats including effects on the fetus.", "content": "Adult virgin female rats were injected daily with low doses (5 or 10 mg/kg) or a high dose (30 mg/kg) of guanethidine for 12 or 18 weeks respectively. 'Short' and 'long' noradrenergic neurones were unaffected by low doeses. This contrasts markedly to earlier findings in male rats in which long-term damage of 'short' noradrenergic neurones occurred, and indicates a basic difference between 'short' noradrenergic neurones in male and female rats. Widespread degeneration of both types of neurones followed treatment with high doses and little reinnervation was observed 8 weeks after cessation of treatment. Fertility, pregnancy and litter size were apparently unaffected. Some teratogenic effects were observed in the offspring of female rats treated with guanethidine (10 or 25 mg/kg/day) before and throughout pregnancy. However, these effects had largely disappeared by the time the offspring were 10 weeks old. Since noradrenergic neurones of newborn rats are particularly sensitive to damage by guanethidine it would appear that either very little guanethidine crosses the placental barrier or that noradrenergic neurones are not susceptible during prenatal development to the cytotoxic effects of guanethidine.", "contents": "Chronic guanethidine treatment of female rats including effects on the fetus. Adult virgin female rats were injected daily with low doses (5 or 10 mg/kg) or a high dose (30 mg/kg) of guanethidine for 12 or 18 weeks respectively. 'Short' and 'long' noradrenergic neurones were unaffected by low doeses. This contrasts markedly to earlier findings in male rats in which long-term damage of 'short' noradrenergic neurones occurred, and indicates a basic difference between 'short' noradrenergic neurones in male and female rats. Widespread degeneration of both types of neurones followed treatment with high doses and little reinnervation was observed 8 weeks after cessation of treatment. Fertility, pregnancy and litter size were apparently unaffected. Some teratogenic effects were observed in the offspring of female rats treated with guanethidine (10 or 25 mg/kg/day) before and throughout pregnancy. However, these effects had largely disappeared by the time the offspring were 10 weeks old. Since noradrenergic neurones of newborn rats are particularly sensitive to damage by guanethidine it would appear that either very little guanethidine crosses the placental barrier or that noradrenergic neurones are not susceptible during prenatal development to the cytotoxic effects of guanethidine."}
{"id": "PMID:480324", "title": "Study of U.S. Medical School Applicants, 1977--78.", "content": "This annual report on applicants to the 1977--78 U.S. medical school freshman class describes the first substantial decline in applicants during the past quarter century. Since the decline was accompanied by the opening of several new medical schools, the chances of gaining admission improved to 39 percent, the highest in six years. For applicants making their first attempt the acceptance rate was 43 percent. There was no loss of qualified applicants, however; the 1977--78 pool presented record-high entrance test scores and undergraduate college grades. In the report comparisons are made with activity in 1974--75--the peak year for medical school spplicants--in order to measure the impact of the decline on individual medical schools and geographical regions and on various applicant subpopulations.", "contents": "Study of U.S. Medical School Applicants, 1977--78. This annual report on applicants to the 1977--78 U.S. medical school freshman class describes the first substantial decline in applicants during the past quarter century. Since the decline was accompanied by the opening of several new medical schools, the chances of gaining admission improved to 39 percent, the highest in six years. For applicants making their first attempt the acceptance rate was 43 percent. There was no loss of qualified applicants, however; the 1977--78 pool presented record-high entrance test scores and undergraduate college grades. In the report comparisons are made with activity in 1974--75--the peak year for medical school spplicants--in order to measure the impact of the decline on individual medical schools and geographical regions and on various applicant subpopulations."}
{"id": "PMID:480325", "title": "Group experience as a means of training medical students.", "content": "All medical students in a six-week psychiatric clerkship met once a week in \"experiential groups.\" One week after the last group session a concluding and evaluative discussion was held. A detailed description of the students' responses and reactions is given. The results indicate that for the majority of the students, the experiential group was the most significant personal and learning experience they had during the clerkship. It effected better interpersonal communication among the students and improved their humane skill, empathy, and sensitivity.", "contents": "Group experience as a means of training medical students. All medical students in a six-week psychiatric clerkship met once a week in \"experiential groups.\" One week after the last group session a concluding and evaluative discussion was held. A detailed description of the students' responses and reactions is given. The results indicate that for the majority of the students, the experiential group was the most significant personal and learning experience they had during the clerkship. It effected better interpersonal communication among the students and improved their humane skill, empathy, and sensitivity."}
{"id": "PMID:480326", "title": "Psychological aspects of medical care: a training seminar for primary care providers.", "content": "A seminar devoted to the psychological aspects of medical care has formed the major portion of the psychiatric training of primary care internal medicine residents at the Kenmore Center of the Harvard Community Health Plan since 1973. The group includes a psychiatrist, a psychiatric nurse, staff physicians, residents, and nurse practitioners. The atmosphere is open and supportive, and discussion focuses on particular situations causing discomfort to members. Content issues include professional identity, relationships with co-workers, and difficulties in handling patients. The group teaches the value of communication and sharing and improves skills in caring for patients.", "contents": "Psychological aspects of medical care: a training seminar for primary care providers. A seminar devoted to the psychological aspects of medical care has formed the major portion of the psychiatric training of primary care internal medicine residents at the Kenmore Center of the Harvard Community Health Plan since 1973. The group includes a psychiatrist, a psychiatric nurse, staff physicians, residents, and nurse practitioners. The atmosphere is open and supportive, and discussion focuses on particular situations causing discomfort to members. Content issues include professional identity, relationships with co-workers, and difficulties in handling patients. The group teaches the value of communication and sharing and improves skills in caring for patients."}
{"id": "PMID:480327", "title": "The first pelvic examination: helping students cope with their emotional reactions.", "content": "The author has conducted seminars with small groups of medical students beginning their training on an obstetrics and gynecology service. Six characteristic responses of students to performing their first pelvic examination are described. Within the framework of the seminar discussion, an attempt is made to help the students cope with those reactions that could impair their ability to perform competent gynecological examinations.", "contents": "The first pelvic examination: helping students cope with their emotional reactions. The author has conducted seminars with small groups of medical students beginning their training on an obstetrics and gynecology service. Six characteristic responses of students to performing their first pelvic examination are described. Within the framework of the seminar discussion, an attempt is made to help the students cope with those reactions that could impair their ability to perform competent gynecological examinations."}
{"id": "PMID:480334", "title": "Electrical properties of the cellular transepithelial pathway in Necturus gallbladder: III. Ionic permeability of the basolateral cell membrane.", "content": "The ionic permeability of the basolateral membrane of Necturus gallbladder epithelium was studied with intracellular microelectrode techniques. After removal of most of the subepithelial tissue (to reduce unstirred layer thickness), impalements were performed from the serosal side, and ionic substitutions were made in the serosal solution while a microelectrode was kept in a cell. Thus, it was possible to obtain continuous (and reversible) records of transepithelial and cell membrane potentials and to measure intermittently the transepithelial resistance and the ratio of cell membrane resistances. From these data and the mean value of the equivalent resistance of the cell membranes in parallel (obtained from cable analysis in a different group of tissues), absolute cell membrane and shunt resistances and equivalent electromotive forces (emfs) were calculated. From the changes of basolateral membrane emf (Eb) produced by the substitutions, the conductance (G) and permeability (P) of the membrane for K, Cl and Na were estimated. Potassium-for-sodium substitutions produced large reductions of both cell membrane potentials, of Eb, and of the resistance of the basolateral membrane (Rb), indicating high GK and PK. Chloride substitution with isethionate or sulfate resulted in smaller changes of cell membrane potentials and Eb and in no significant change of Rb, indicating small but measurable values of GCl and PCl. Sodium substitutions with N-methyl-D-glucamine (NMDG) resulted in cell potential changes entirely attributable to the biionic potential produced in the shunt pathway (PNa greater than PNMDG), and in no significant changes of Rb or Eb, indicating that GNa and PNa are undetectable. The question of the mechanism of Cl transport across the basolateral membrane was addressed by comparing the mean rate of transepithelial Cl transport : formula, see text: and the predicted passive Cl flux across the basolateral membrane (from the membrane Cl conductance, potential, and Cl equilibrium potential). The conclusion is that only a very small fraction of the Cl flux across the basolateral membrane can be electrodiffusional. Since the paracellular Cl conductance is also too low to account for : formula, see text:, these results suggest the presence of a neutral mechanism of Cl extrusion from the cells. This could be a NaCl pump, a downhill KCl transport mechanism, or a Cl-HCO3 exchange mechanism.", "contents": "Electrical properties of the cellular transepithelial pathway in Necturus gallbladder: III. Ionic permeability of the basolateral cell membrane. The ionic permeability of the basolateral membrane of Necturus gallbladder epithelium was studied with intracellular microelectrode techniques. After removal of most of the subepithelial tissue (to reduce unstirred layer thickness), impalements were performed from the serosal side, and ionic substitutions were made in the serosal solution while a microelectrode was kept in a cell. Thus, it was possible to obtain continuous (and reversible) records of transepithelial and cell membrane potentials and to measure intermittently the transepithelial resistance and the ratio of cell membrane resistances. From these data and the mean value of the equivalent resistance of the cell membranes in parallel (obtained from cable analysis in a different group of tissues), absolute cell membrane and shunt resistances and equivalent electromotive forces (emfs) were calculated. From the changes of basolateral membrane emf (Eb) produced by the substitutions, the conductance (G) and permeability (P) of the membrane for K, Cl and Na were estimated. Potassium-for-sodium substitutions produced large reductions of both cell membrane potentials, of Eb, and of the resistance of the basolateral membrane (Rb), indicating high GK and PK. Chloride substitution with isethionate or sulfate resulted in smaller changes of cell membrane potentials and Eb and in no significant change of Rb, indicating small but measurable values of GCl and PCl. Sodium substitutions with N-methyl-D-glucamine (NMDG) resulted in cell potential changes entirely attributable to the biionic potential produced in the shunt pathway (PNa greater than PNMDG), and in no significant changes of Rb or Eb, indicating that GNa and PNa are undetectable. The question of the mechanism of Cl transport across the basolateral membrane was addressed by comparing the mean rate of transepithelial Cl transport : formula, see text: and the predicted passive Cl flux across the basolateral membrane (from the membrane Cl conductance, potential, and Cl equilibrium potential). The conclusion is that only a very small fraction of the Cl flux across the basolateral membrane can be electrodiffusional. Since the paracellular Cl conductance is also too low to account for : formula, see text:, these results suggest the presence of a neutral mechanism of Cl extrusion from the cells. This could be a NaCl pump, a downhill KCl transport mechanism, or a Cl-HCO3 exchange mechanism."}
{"id": "PMID:480335", "title": "Slowing of the time course of the excitation of squid giant axons in viscous solutions.", "content": "The time course of excitation of intracellularly perfused squid giant axons was slowed as the solution viscosity was raised by adding neutral molecules, i.e., glucose and glycerol. By twofold increase of the solution viscosity, the duration of action potential was prolonged to 2.7-fold and the maximum rate of rise decreased to one-half. At the same time, the membrane resistance at resting state increased by 60%. These effects were reversible. The time course of inward and outward currents was slowed also. When the solution viscosity increased to twofold, the time to peak inward current increased by 80%, and the amplitudes of peak inward and steady outward currents decreased by 60% and by 70%, respectively. These effects were not specific for the sodium or the potassium channel. Effects of solution viscosity occurred in both hypotonic and hypertonic solutions. Q10 values of temperature dependence of the time course of the action potential were equal in any viscous solutions. These effects in viscous solutions were explained by the change in solution viscosity but not by the change in solution osmolarities, ionic activities, or solution resistivity.", "contents": "Slowing of the time course of the excitation of squid giant axons in viscous solutions. The time course of excitation of intracellularly perfused squid giant axons was slowed as the solution viscosity was raised by adding neutral molecules, i.e., glucose and glycerol. By twofold increase of the solution viscosity, the duration of action potential was prolonged to 2.7-fold and the maximum rate of rise decreased to one-half. At the same time, the membrane resistance at resting state increased by 60%. These effects were reversible. The time course of inward and outward currents was slowed also. When the solution viscosity increased to twofold, the time to peak inward current increased by 80%, and the amplitudes of peak inward and steady outward currents decreased by 60% and by 70%, respectively. These effects were not specific for the sodium or the potassium channel. Effects of solution viscosity occurred in both hypotonic and hypertonic solutions. Q10 values of temperature dependence of the time course of the action potential were equal in any viscous solutions. These effects in viscous solutions were explained by the change in solution viscosity but not by the change in solution osmolarities, ionic activities, or solution resistivity."}
{"id": "PMID:480336", "title": "Reversible electrical breakdown of lipid bilayer membranes: a charge-pulse relaxation study.", "content": "Charge-pulse experiments were performed with lipid bilayer membranes from oxidized cholesterol/n-decane at relatively high voltages (several hundred mV). The membranes show an irreversible mechanical rupture if the membrane is charged to voltages on the order of 300 mV. In the case of the mechanical rupture, the voltage across the membrane needs about 50-200 musec to decay completely to zero. At much higher voltages, applied to the membrane by charge pulses of about 500 nsec duration, a decrease of the specific resistance of the membranes by nine orders of magnitude is observed (from 10(8) to 0.1 omega cm2), which is correlated with the reversible electrical breakdown of the lipid bilayer membrane. Due to the high conductance increase (breakdown) of the bilayer it is not possible to charge the membrane to a larger value than the critical potential difference Vc. For 1 M alkali ion chlorides Vc was about 1 V. The temperature dependence of the electrical breakdown voltage Vc is comparable to that being observed with cell membranes. Vc decreases between 2 and 48 degrees C from 1.5 to 0.6 V in the presence of 1 M KCl. Breakdown experiments were also performed with lipid bilayer membrane composed of other lipids. The fast decay of the voltage (current) in the 100-nsec range after application of a charge pulse was very similar in these experiments compared with experiments with membranes made from oxidized cholesterol. However, the membranes made from other lipids show a mechanical breakdown after the electrical breakdown, whereas with one single membrane from oxidized cholesterol more than twenty reproducible breakdown experiments could be repeated without a visible disturbance of the membrane stability. The reversible electrical breakdown of the membrane is discussed in terms of both compression of the membrane (electromechanical model) and ion movement through the membrane induced by high electric field strength (Born energy).", "contents": "Reversible electrical breakdown of lipid bilayer membranes: a charge-pulse relaxation study. Charge-pulse experiments were performed with lipid bilayer membranes from oxidized cholesterol/n-decane at relatively high voltages (several hundred mV). The membranes show an irreversible mechanical rupture if the membrane is charged to voltages on the order of 300 mV. In the case of the mechanical rupture, the voltage across the membrane needs about 50-200 musec to decay completely to zero. At much higher voltages, applied to the membrane by charge pulses of about 500 nsec duration, a decrease of the specific resistance of the membranes by nine orders of magnitude is observed (from 10(8) to 0.1 omega cm2), which is correlated with the reversible electrical breakdown of the lipid bilayer membrane. Due to the high conductance increase (breakdown) of the bilayer it is not possible to charge the membrane to a larger value than the critical potential difference Vc. For 1 M alkali ion chlorides Vc was about 1 V. The temperature dependence of the electrical breakdown voltage Vc is comparable to that being observed with cell membranes. Vc decreases between 2 and 48 degrees C from 1.5 to 0.6 V in the presence of 1 M KCl. Breakdown experiments were also performed with lipid bilayer membrane composed of other lipids. The fast decay of the voltage (current) in the 100-nsec range after application of a charge pulse was very similar in these experiments compared with experiments with membranes made from oxidized cholesterol. However, the membranes made from other lipids show a mechanical breakdown after the electrical breakdown, whereas with one single membrane from oxidized cholesterol more than twenty reproducible breakdown experiments could be repeated without a visible disturbance of the membrane stability. The reversible electrical breakdown of the membrane is discussed in terms of both compression of the membrane (electromechanical model) and ion movement through the membrane induced by high electric field strength (Born energy)."}
{"id": "PMID:480337", "title": "Volume flows across gallbladder epithelium induced by small hydrostatic and osmotic gradients.", "content": "The hydraulic conductivity of rabbit gallbladder epithelium has been studied using a continuous volumetric method based on capacitance measurements. The time resolution for measuring osmotic flows is in the range of seconds. Volume flows have been induced by osmotic gradients between 0 and 100 mosmol. In this range the flow-force relation is linear and the Pf value is 9.3 X 10(-3) cm/sec. After correction for solute polarization effects, the Pf value amounts to 0.05 cm/sec. The observed flow is constant between 5 sec up to 20 min after a sudden increase in the osmolarity of the mucosal solution. The wet weight of the gallbladder tissue decreases by 22% and increases by 30% during osmotic flows from mucosa to serosa, respectively. Volume flows induced by hydrostatic pressure gradients on the mucosal surface are linearly related to the driving forces between 0 and 40 mbar. The Pf value is 0.15 cm/sec. The volume flows are constant between 2 sec and 15 min after pressure application. The flow-force relation for pressure gradients on the serosal surface is markedly nonlinear for gradients greater than 5 mbar. Below 5 mbar the Pf value is 4.5 cm/sec. From electrical measurements, e.g., resistance and streaming potentials, and from flux studies with inulin and polyethylene glycol 4000, it is concluded that hydrostatic and osmotic gradients are not comparable when they are applied to gallbladder epithelium. They induce volume flows across different pathways, e.g., osmosis predominantly across the cellular route and pressure filtration predominantly across paracellular routes.", "contents": "Volume flows across gallbladder epithelium induced by small hydrostatic and osmotic gradients. The hydraulic conductivity of rabbit gallbladder epithelium has been studied using a continuous volumetric method based on capacitance measurements. The time resolution for measuring osmotic flows is in the range of seconds. Volume flows have been induced by osmotic gradients between 0 and 100 mosmol. In this range the flow-force relation is linear and the Pf value is 9.3 X 10(-3) cm/sec. After correction for solute polarization effects, the Pf value amounts to 0.05 cm/sec. The observed flow is constant between 5 sec up to 20 min after a sudden increase in the osmolarity of the mucosal solution. The wet weight of the gallbladder tissue decreases by 22% and increases by 30% during osmotic flows from mucosa to serosa, respectively. Volume flows induced by hydrostatic pressure gradients on the mucosal surface are linearly related to the driving forces between 0 and 40 mbar. The Pf value is 0.15 cm/sec. The volume flows are constant between 2 sec and 15 min after pressure application. The flow-force relation for pressure gradients on the serosal surface is markedly nonlinear for gradients greater than 5 mbar. Below 5 mbar the Pf value is 4.5 cm/sec. From electrical measurements, e.g., resistance and streaming potentials, and from flux studies with inulin and polyethylene glycol 4000, it is concluded that hydrostatic and osmotic gradients are not comparable when they are applied to gallbladder epithelium. They induce volume flows across different pathways, e.g., osmosis predominantly across the cellular route and pressure filtration predominantly across paracellular routes."}
{"id": "PMID:480338", "title": "Characteristics of anion transport in cat and dog red blood cells.", "content": "Self-exchange of chloride and sulfate in dog and cat red cells has been measured under equilibrium conditions. The rats of efflux for these anions are approximately twofold higher in dog compared to cat red blood cells. Although the rates differ, the anion exchange systems of these two red cell types exhibit many common properties. The dependence of 35SO4 efflux on the intracellular SO4 concentration, the pH dependence and the inhibition of 35SO4 efflux by Cl and SITS are almost identical in dog and cat red cells. Nystatin treatment was used to study the dependence of 36Cl efflux on internal Cl. Chloride efflux exhibits saturation in both cell types with dog red cells possessing a higher Vmax and K1/2 than cat red cells. The number of anion transport sites was estimated by extrapolation to the number of molecules of dihydro DIDS (H2DIDS, where DIDS is 4,4'-diisothiocyano-2,2' stilbene-disulfonic acid) which were bound at 100% inhibition of transport. The results indicate that either the turnover numbers for anion transport differ in dog, cat, and human red cells or that there is heterogeneity in the function of the membrane components which bind H2DIDS.", "contents": "Characteristics of anion transport in cat and dog red blood cells. Self-exchange of chloride and sulfate in dog and cat red cells has been measured under equilibrium conditions. The rats of efflux for these anions are approximately twofold higher in dog compared to cat red blood cells. Although the rates differ, the anion exchange systems of these two red cell types exhibit many common properties. The dependence of 35SO4 efflux on the intracellular SO4 concentration, the pH dependence and the inhibition of 35SO4 efflux by Cl and SITS are almost identical in dog and cat red cells. Nystatin treatment was used to study the dependence of 36Cl efflux on internal Cl. Chloride efflux exhibits saturation in both cell types with dog red cells possessing a higher Vmax and K1/2 than cat red cells. The number of anion transport sites was estimated by extrapolation to the number of molecules of dihydro DIDS (H2DIDS, where DIDS is 4,4'-diisothiocyano-2,2' stilbene-disulfonic acid) which were bound at 100% inhibition of transport. The results indicate that either the turnover numbers for anion transport differ in dog, cat, and human red cells or that there is heterogeneity in the function of the membrane components which bind H2DIDS."}
{"id": "PMID:480339", "title": "Fluorescent probes for asymmetric lipid bilayers: synthesis and properties in phosphatidyl choline liposomes and erythrocyte membranes.", "content": "We have synthesized three sets of fluorescent probes which we believe will be useful in studies of asymmetric membranes and have studied their interactions with model lipid bilayers and erythrocyte membranes. The probes were designed to partition preferentially into one face of a lipid bilayer with asymmetrically disposed phospholipids and to report lipid transitions in that monolayer. We synthesized more than twenty probes containing anthroyl-, dansyl-, or pyrene rings with acidic, basic, and neutral functional groups and alkyl \"spacers\" of various lengths. The interactions of these probes with liposomes of phosphatidyl choline and with erythrocyte membranes were characterized to determine whether probe insertion was asymmetric, how deeply the probe penetrated the bilayer, and whether the probe reflected thermotropic phase transitions in model membranes. The set of variously charged anthroyl esters, analogs of local anaesthetics, appears to be promising for studies of asymmetric membranes. Fluorescent probes have been used extensively to provide information on the lipid regions of biological membranes. Membrane fluidity, a composite of molecular packing and motion of acyl chains in lipid bilayers, has been assessed with a variety of fluorescent probes, the fluorescence of which undergoes some measurable change at the temperature of the membrane's thermotropic phase transition. A large number of fluorescent probes have been used for this purpose. Bashford, Morgan and Radda (Bashford, C.L., Morgan, C.G., Radda, G.K. 1976; Biochim. Biophys. Acta 426:157) and Thulborn and Sawyer (Thulborn, K.R., Sawyer, W. H. 1978; Biochim. Biophys. Acta 511:125) synthesized several fatty acid derivatives in which an anthracene group is attached (in ester linkage) along the acyl chain at various positions, and have shown that this set of probes may be useful in probing membrane fluidity at different depths within the bilayer. This report  describes the synthesis and properties of several sets of amphipathic fluorescent probes, which may partition unequally into the two faces of an asymmetric lipid bilayer, and may therefore provide information about membranes complementary to that obtainable with existing probes.", "contents": "Fluorescent probes for asymmetric lipid bilayers: synthesis and properties in phosphatidyl choline liposomes and erythrocyte membranes. We have synthesized three sets of fluorescent probes which we believe will be useful in studies of asymmetric membranes and have studied their interactions with model lipid bilayers and erythrocyte membranes. The probes were designed to partition preferentially into one face of a lipid bilayer with asymmetrically disposed phospholipids and to report lipid transitions in that monolayer. We synthesized more than twenty probes containing anthroyl-, dansyl-, or pyrene rings with acidic, basic, and neutral functional groups and alkyl \"spacers\" of various lengths. The interactions of these probes with liposomes of phosphatidyl choline and with erythrocyte membranes were characterized to determine whether probe insertion was asymmetric, how deeply the probe penetrated the bilayer, and whether the probe reflected thermotropic phase transitions in model membranes. The set of variously charged anthroyl esters, analogs of local anaesthetics, appears to be promising for studies of asymmetric membranes. Fluorescent probes have been used extensively to provide information on the lipid regions of biological membranes. Membrane fluidity, a composite of molecular packing and motion of acyl chains in lipid bilayers, has been assessed with a variety of fluorescent probes, the fluorescence of which undergoes some measurable change at the temperature of the membrane's thermotropic phase transition. A large number of fluorescent probes have been used for this purpose. Bashford, Morgan and Radda (Bashford, C.L., Morgan, C.G., Radda, G.K. 1976; Biochim. Biophys. Acta 426:157) and Thulborn and Sawyer (Thulborn, K.R., Sawyer, W. H. 1978; Biochim. Biophys. Acta 511:125) synthesized several fatty acid derivatives in which an anthracene group is attached (in ester linkage) along the acyl chain at various positions, and have shown that this set of probes may be useful in probing membrane fluidity at different depths within the bilayer. This report  describes the synthesis and properties of several sets of amphipathic fluorescent probes, which may partition unequally into the two faces of an asymmetric lipid bilayer, and may therefore provide information about membranes complementary to that obtainable with existing probes."}
{"id": "PMID:480340", "title": "Zinc-dependent action potentials in giant neurons of the snail, Euhadra quaestia.", "content": "In giant neurons of subesophageal ganglion of the Japanese land snail, Euhadra quaestia Deshayes, permeation of Zn ions through Ca channels were investigated with a conventional current clamp method. All-or-none action potentials of long duration (90 to 120 sec) were evoked in 24 mM Zn containing salines. The overshoots were about +10 mV and the maximum rate of rises (MRRs) was about 2.9 V/sec. The amplitudes and the MRRs of the action potentials depended on external Zn ion concentrations. The action potentials were suppressed by specific Ca-channel inhibitors such as Co2+, La3+ and Verapamil, but they were resistant to Na-channel inhibitor, tetrodotoxin, even at 30 microM. It is concluded that these action potentials are generated by Zn ions permeating Ca channels in snail neuronal membrane. On the basis of Hagiwara and Takahashi's (S. Hagiwara & K. Takahashi, 1967, J. Gen. Physiol. 50:583) model of Ca channels, it is inferred that Zn ions are 5 to 10 times stronger in affinity to Ca channels than Ca ions, but 10 to 20 times less permeable.", "contents": "Zinc-dependent action potentials in giant neurons of the snail, Euhadra quaestia. In giant neurons of subesophageal ganglion of the Japanese land snail, Euhadra quaestia Deshayes, permeation of Zn ions through Ca channels were investigated with a conventional current clamp method. All-or-none action potentials of long duration (90 to 120 sec) were evoked in 24 mM Zn containing salines. The overshoots were about +10 mV and the maximum rate of rises (MRRs) was about 2.9 V/sec. The amplitudes and the MRRs of the action potentials depended on external Zn ion concentrations. The action potentials were suppressed by specific Ca-channel inhibitors such as Co2+, La3+ and Verapamil, but they were resistant to Na-channel inhibitor, tetrodotoxin, even at 30 microM. It is concluded that these action potentials are generated by Zn ions permeating Ca channels in snail neuronal membrane. On the basis of Hagiwara and Takahashi's (S. Hagiwara & K. Takahashi, 1967, J. Gen. Physiol. 50:583) model of Ca channels, it is inferred that Zn ions are 5 to 10 times stronger in affinity to Ca channels than Ca ions, but 10 to 20 times less permeable."}
{"id": "PMID:480342", "title": "Relationship of California group virus infection and mental retardation: seroepidemiological observations.", "content": "A plaque reduction neutralisation test was utilised to determine the prevalence of antibodies to California group virus (LaCrosse subtype) among 612 moderately to profoundly retarded subjects resident in two Wisconsin state institutions. Neutralising antibodies were detected in the sera of fifty-one (8 per cent) of the subjects tested. Age and sex differences in antibody distribution were not significant. When sero-logical data correlated with clinical case history records, antibody was found in the sera of 13 per cent (24/188) of a group in which mental retardation (MR) was attributed to \"presumed psychological causes\" and in 8 per cent (12/158) of a group for which the aetiology of MR was \"encephalopathy\". In a group of 254 subjects with MR of unknown aetiology, none of the sixty subjects with cranial anomaly and only two of the ninety-five (2.1 per cent) subjects with Down's syndrome were positive for antibody to CEV, while thirteen of ninety-nine (13.1 per cent) subjects diagnosed as \"other cerebral defects\" had CEV antibody. In a majority of the subjects with CEV neutralising antibody, MR was attributed to perinatal influences.", "contents": "Relationship of California group virus infection and mental retardation: seroepidemiological observations. A plaque reduction neutralisation test was utilised to determine the prevalence of antibodies to California group virus (LaCrosse subtype) among 612 moderately to profoundly retarded subjects resident in two Wisconsin state institutions. Neutralising antibodies were detected in the sera of fifty-one (8 per cent) of the subjects tested. Age and sex differences in antibody distribution were not significant. When sero-logical data correlated with clinical case history records, antibody was found in the sera of 13 per cent (24/188) of a group in which mental retardation (MR) was attributed to \"presumed psychological causes\" and in 8 per cent (12/158) of a group for which the aetiology of MR was \"encephalopathy\". In a group of 254 subjects with MR of unknown aetiology, none of the sixty subjects with cranial anomaly and only two of the ninety-five (2.1 per cent) subjects with Down's syndrome were positive for antibody to CEV, while thirteen of ninety-nine (13.1 per cent) subjects diagnosed as \"other cerebral defects\" had CEV antibody. In a majority of the subjects with CEV neutralising antibody, MR was attributed to perinatal influences."}
{"id": "PMID:480341", "title": "Intracellular ionic activities and transmembrane electrochemical potential differences in gallbladder epithelium.", "content": "Intracellular ion activities in Necturus gallbladder epithelium were measured with liquid ion-exchanger microelectrodes. Mean values for K, Cl and Na activities were 87, 35 and 22 mM, respectively. The intracellular activities of both K and Cl are above their respective equilibrium values, whereas the Na activity is far below. This indicates that K and Cl are transported uphill toward the cell interior, whereas Na is extruded against its electrochemical gradient. The epithelium transports NaCl from mucosa to serosa. From the data presented and the known Na and Cl conductances of the cell membranes, we conclude that neutral transport driven by the Na electrochemical potential difference can account for NaCl entry at the apical membrane. At the basolateral membrane, Na is actively transported. Because of the low Cl conductance of the membrane, only a small fraction of Cl transport can be explained by diffusion. These data suggest that Cl transport across the basolateral membrane is a coupled process which involves a neutral NaCl pump, downhill KCl transport, or a Cl-anion exchange system.", "contents": "Intracellular ionic activities and transmembrane electrochemical potential differences in gallbladder epithelium. Intracellular ion activities in Necturus gallbladder epithelium were measured with liquid ion-exchanger microelectrodes. Mean values for K, Cl and Na activities were 87, 35 and 22 mM, respectively. The intracellular activities of both K and Cl are above their respective equilibrium values, whereas the Na activity is far below. This indicates that K and Cl are transported uphill toward the cell interior, whereas Na is extruded against its electrochemical gradient. The epithelium transports NaCl from mucosa to serosa. From the data presented and the known Na and Cl conductances of the cell membranes, we conclude that neutral transport driven by the Na electrochemical potential difference can account for NaCl entry at the apical membrane. At the basolateral membrane, Na is actively transported. Because of the low Cl conductance of the membrane, only a small fraction of Cl transport can be explained by diffusion. These data suggest that Cl transport across the basolateral membrane is a coupled process which involves a neutral NaCl pump, downhill KCl transport, or a Cl-anion exchange system."}
{"id": "PMID:480343", "title": "A social learning approach to vocational training of the severely retarded.", "content": "Seven severely retarded adults from a small rural developmental centre were treated for in appropriate vocational behaviour in the centre's workshop. Target types of behaviour were accurate and efficient performance of three types of operationally defined work behaviour collectively called work skills. Specific inappropriate behaviour for individual residents included chin wiping, inappropriate touching, and eyes off task. Training involved providing instructions, performance feedback, role-playing, and social and monetary reinforcers, which resulted in improvement in all types of targeted behaviour. Follow-up four weeks after treatment was conducted revealed a maintenance of gains on target behaviour.", "contents": "A social learning approach to vocational training of the severely retarded. Seven severely retarded adults from a small rural developmental centre were treated for in appropriate vocational behaviour in the centre's workshop. Target types of behaviour were accurate and efficient performance of three types of operationally defined work behaviour collectively called work skills. Specific inappropriate behaviour for individual residents included chin wiping, inappropriate touching, and eyes off task. Training involved providing instructions, performance feedback, role-playing, and social and monetary reinforcers, which resulted in improvement in all types of targeted behaviour. Follow-up four weeks after treatment was conducted revealed a maintenance of gains on target behaviour."}
{"id": "PMID:480344", "title": "Digital picture analysis in microphotometry of biological materials.", "content": "Automatic evaluation of two-dimensional thin-layer-chromatograms exemplarily demonstrates the applicability of signal processing, image analysis, and pattern recognition to microphotometry by an enlarged dynamic range of the photometer, improved reproducibility by noise reduction, discrimination between objects and background, feature extraction and automatic classification of the results. Emphasis is put on simple procedures which are tested in routine work and can be implemented on minicomputers.", "contents": "Digital picture analysis in microphotometry of biological materials. Automatic evaluation of two-dimensional thin-layer-chromatograms exemplarily demonstrates the applicability of signal processing, image analysis, and pattern recognition to microphotometry by an enlarged dynamic range of the photometer, improved reproducibility by noise reduction, discrimination between objects and background, feature extraction and automatic classification of the results. Emphasis is put on simple procedures which are tested in routine work and can be implemented on minicomputers."}
{"id": "PMID:480345", "title": "Spectral changes of the fluorescent acriflavine--(Feulgen) DNA dye complex at different temperatures: application of a new cryostat for measurements in reflected and transmitted light.", "content": "The fluorometric behaviour of cellular objects is influenced during excitation by two nearly independent phenomena: (1) by the photochemical reaction of the DNA/AF dye complex, and (2) by the energy transfer among several DNA/AF dye complexes. Both processes show a distinct temperature-dependent behaviour and can therefore be characterized by the analysis of the fluorescence spectra at different temperatures. All microfluorometric measurements were performed with a self-constructed cooling device. The cryostat permits measurements of the cellular fluorescence within a range of temperatures between 4 K and 300 K. The cooling unit operates in accordance with the 'Continuous Flow Principle' and allows the application of objectives up to a numerical aperture of 0.6.", "contents": "Spectral changes of the fluorescent acriflavine--(Feulgen) DNA dye complex at different temperatures: application of a new cryostat for measurements in reflected and transmitted light. The fluorometric behaviour of cellular objects is influenced during excitation by two nearly independent phenomena: (1) by the photochemical reaction of the DNA/AF dye complex, and (2) by the energy transfer among several DNA/AF dye complexes. Both processes show a distinct temperature-dependent behaviour and can therefore be characterized by the analysis of the fluorescence spectra at different temperatures. All microfluorometric measurements were performed with a self-constructed cooling device. The cryostat permits measurements of the cellular fluorescence within a range of temperatures between 4 K and 300 K. The cooling unit operates in accordance with the 'Continuous Flow Principle' and allows the application of objectives up to a numerical aperture of 0.6."}
{"id": "PMID:480368", "title": "Synthesis of amino acyl adenylates using the tert-butoxycarbonyl protecting group.", "content": "Alayl, [ 14c]-alanyl, phenylalanyl, and methionyl adenylates have been synthesized in high yields and relatively good purities. Elemental analysis 1H nmr and ir spectra have been utilized for the characterization of these extremely labile compounds. The present synthesis, which uses readily available N-tert-butoxycarbonyl amino acids, is compared with previous methods.", "contents": "Synthesis of amino acyl adenylates using the tert-butoxycarbonyl protecting group. Alayl, [ 14c]-alanyl, phenylalanyl, and methionyl adenylates have been synthesized in high yields and relatively good purities. Elemental analysis 1H nmr and ir spectra have been utilized for the characterization of these extremely labile compounds. The present synthesis, which uses readily available N-tert-butoxycarbonyl amino acids, is compared with previous methods."}
{"id": "PMID:480369", "title": "Inadequacy of prebiotic synthesis as origin of proteinous amino acids.", "content": "The production of some nonproteinous, and lack of production of other proteinous, amino acids in model prebiotic synthesis, along with the instability of glutamine and asparagine, suggest that not all of the 20 present day proteinous amino acids gained entry into proteins directly from the primordial soup. Instead, a process of active co-evolution of the genetic code and its constituent amino acids would have to precede the final selection of these proteinous amono acids.", "contents": "Inadequacy of prebiotic synthesis as origin of proteinous amino acids. The production of some nonproteinous, and lack of production of other proteinous, amino acids in model prebiotic synthesis, along with the instability of glutamine and asparagine, suggest that not all of the 20 present day proteinous amino acids gained entry into proteins directly from the primordial soup. Instead, a process of active co-evolution of the genetic code and its constituent amino acids would have to precede the final selection of these proteinous amono acids."}
{"id": "PMID:480370", "title": "A graph theoretic approach to the development of minimal phylogenetic trees.", "content": "The problem of determining the minimal phylogenetic tree is discussed in relation to graph theory. It is shown that this problem is an example of the Steiner problem in graphs which is to connect a set of points by a minimal length network where new points can be added. There is no reported method of solving realistically-sized Steiner problems in reasonable computing time. A heuristic method of approaching the phylogenetic problem is presented, together with a worked example with 7 mammalian cytochrome c sequences. It is shown in this case that the method develops a phylogenetic tree that has the smallest possible number of amino acid replacements. The potential and limitations of the method are discussed. It is stressed that objective methods must be used for comparing different trees. In particular it should be determined how close a given tree is to a mathematically determined lower bound. A theorem is proved which is used to establish a lower bound on the lenghtof any tree and if a tree is found with a length equal to the lower bound, then no shorter tree can exist.", "contents": "A graph theoretic approach to the development of minimal phylogenetic trees. The problem of determining the minimal phylogenetic tree is discussed in relation to graph theory. It is shown that this problem is an example of the Steiner problem in graphs which is to connect a set of points by a minimal length network where new points can be added. There is no reported method of solving realistically-sized Steiner problems in reasonable computing time. A heuristic method of approaching the phylogenetic problem is presented, together with a worked example with 7 mammalian cytochrome c sequences. It is shown in this case that the method develops a phylogenetic tree that has the smallest possible number of amino acid replacements. The potential and limitations of the method are discussed. It is stressed that objective methods must be used for comparing different trees. In particular it should be determined how close a given tree is to a mathematically determined lower bound. A theorem is proved which is used to establish a lower bound on the lenghtof any tree and if a tree is found with a length equal to the lower bound, then no shorter tree can exist."}
{"id": "PMID:480373", "title": "A proposal concerning the origin of life on the planet earth.", "content": "The widely accepted Oparin thesis for the origin and early evolution of life seems sufficiently far from the true state of affairs as to be considered incorrect. It is proposed that life on earth actually arose in the planet's atmosphere, however an atmosphere very different from the present one. Because of an extremely warm surface, the early earth may have possessed no liquid surface water, its water being partitioned between a motten crust and a fairly dense atmosphere. Early preliving systems are taken to arise in the droplet phase in such an atmosphere. The early earth, which resembled Venus then and to some extent now, underwent a transition to its present condition largely as a result of the evolution of methanogenic metabolism.", "contents": "A proposal concerning the origin of life on the planet earth. The widely accepted Oparin thesis for the origin and early evolution of life seems sufficiently far from the true state of affairs as to be considered incorrect. It is proposed that life on earth actually arose in the planet's atmosphere, however an atmosphere very different from the present one. Because of an extremely warm surface, the early earth may have possessed no liquid surface water, its water being partitioned between a motten crust and a fairly dense atmosphere. Early preliving systems are taken to arise in the droplet phase in such an atmosphere. The early earth, which resembled Venus then and to some extent now, underwent a transition to its present condition largely as a result of the evolution of methanogenic metabolism."}
{"id": "PMID:480374", "title": "Induction of glucagon synthesis in diabetic CBA mice bearing mammary aplastic carcinomas.", "content": "Passaging of the mammary aplastic carcinoma several times in diabetic CBA mice induced an extrapancreatic secretion of glucagon. Consequently, the concentration of immunoreactive glucagon was higher in plasma and the tumor tissue extract. After the injection of alloxan, attempts to reduce the level of this hormone in the diabetic mice were unsuccessful. The tumor cells could have been responsible for the secretion of glucagon.", "contents": "Induction of glucagon synthesis in diabetic CBA mice bearing mammary aplastic carcinomas. Passaging of the mammary aplastic carcinoma several times in diabetic CBA mice induced an extrapancreatic secretion of glucagon. Consequently, the concentration of immunoreactive glucagon was higher in plasma and the tumor tissue extract. After the injection of alloxan, attempts to reduce the level of this hormone in the diabetic mice were unsuccessful. The tumor cells could have been responsible for the secretion of glucagon."}
{"id": "PMID:480376", "title": "Qualitative changes in the biologic characteristics of cultured fetal rat keratinizing epidermal cells during the process of malignant transformation after benzo[a]pyrene treatment.", "content": "In vitro malignant transformation of fetal rat keratinizing epidermal cells from inbred SD rats after benzo[a]pyrene (BP) treatment was analyzed from various biologic viewpoints. BP treatment directly and indirectly effected changes in cell growth characteristics, i.e., temperature dependence for growth, in vitro keratinization, chromosome structure, and the ability to form colonies on plastic substrate, on 0.57% agar medium layer, and in 0.33% soft agar medium. BP-treated cells at 30 degrees C remained in the premalignant stages and showed shifts in chromosome structure toward the hypodiploid range and parakeratotic changes in their keratinization process. However, the cells failed to form colonies even on a plastic substrate. BP-treated cell lines that adapted to temperatures of 35 and 37.5 degrees C remained in the premalignant stages; however, they acquired the ability to form colonies on plastic substrates during subcultivation. Malignantly transformed colonies appeared in these cell lines. In vitro keratinization processes were classified into nearly normal (diffuse lamellar, focal lamellar, and parakeratotic), intermediate, and atypical subtypes (columnar, spherical, and single-cell type). Cells of atypical keratinization subtypes and some of the intermediate subtypes formed squamous cell carcinomas in syngeneic hosts. Malignantly transformed cells showed shifts in chromosome structure toward the hypotetraploid range and colony formation on the 0.57% agar medium layer. However, they failed to form colonies in 0.33% soft agar medium. With the use of changes in biologic characteristics of the cells as indicators, fetal rat keratinizing epidermal cells in culture were classified into five stages. The appearance of stage III cells seemed to be the first key step in their malignant transformation.", "contents": "Qualitative changes in the biologic characteristics of cultured fetal rat keratinizing epidermal cells during the process of malignant transformation after benzo[a]pyrene treatment. In vitro malignant transformation of fetal rat keratinizing epidermal cells from inbred SD rats after benzo[a]pyrene (BP) treatment was analyzed from various biologic viewpoints. BP treatment directly and indirectly effected changes in cell growth characteristics, i.e., temperature dependence for growth, in vitro keratinization, chromosome structure, and the ability to form colonies on plastic substrate, on 0.57% agar medium layer, and in 0.33% soft agar medium. BP-treated cells at 30 degrees C remained in the premalignant stages and showed shifts in chromosome structure toward the hypodiploid range and parakeratotic changes in their keratinization process. However, the cells failed to form colonies even on a plastic substrate. BP-treated cell lines that adapted to temperatures of 35 and 37.5 degrees C remained in the premalignant stages; however, they acquired the ability to form colonies on plastic substrates during subcultivation. Malignantly transformed colonies appeared in these cell lines. In vitro keratinization processes were classified into nearly normal (diffuse lamellar, focal lamellar, and parakeratotic), intermediate, and atypical subtypes (columnar, spherical, and single-cell type). Cells of atypical keratinization subtypes and some of the intermediate subtypes formed squamous cell carcinomas in syngeneic hosts. Malignantly transformed cells showed shifts in chromosome structure toward the hypotetraploid range and colony formation on the 0.57% agar medium layer. However, they failed to form colonies in 0.33% soft agar medium. With the use of changes in biologic characteristics of the cells as indicators, fetal rat keratinizing epidermal cells in culture were classified into five stages. The appearance of stage III cells seemed to be the first key step in their malignant transformation."}
{"id": "PMID:480377", "title": "Rapid in vivo assay of mouse natural killer cell activity.", "content": "A rapid elimination of tumor cells from some organs was detected in mice following iv injection of tumor cells labeld in vitro with [125I]5-iodo-2'-deoxyuridine. Recovery of radioactivity in different organs (spleen, liver, and lungs) was reduced in mice with high natural killer (NK) cell reactivity in their spleens, as measured in vitro by concomitant short-term 51Cr release assay. Considerable parallelism between in vitro and in vivo reactivities against two mouse lymphomas and a human myeloid cell line was found in mice of different strains and ages. Similarly, various immunophamacologic treatments had comparable effects on in vitro and in vivo reactivities. These findings are consistent with the hypothesis that rapid cytolysis of tumor cells occurred in vivo and that NK cells played a major role in their elimination.", "contents": "Rapid in vivo assay of mouse natural killer cell activity. A rapid elimination of tumor cells from some organs was detected in mice following iv injection of tumor cells labeld in vitro with [125I]5-iodo-2'-deoxyuridine. Recovery of radioactivity in different organs (spleen, liver, and lungs) was reduced in mice with high natural killer (NK) cell reactivity in their spleens, as measured in vitro by concomitant short-term 51Cr release assay. Considerable parallelism between in vitro and in vivo reactivities against two mouse lymphomas and a human myeloid cell line was found in mice of different strains and ages. Similarly, various immunophamacologic treatments had comparable effects on in vitro and in vivo reactivities. These findings are consistent with the hypothesis that rapid cytolysis of tumor cells occurred in vivo and that NK cells played a major role in their elimination."}
{"id": "PMID:480379", "title": "Enhancement of experimental tumors in mice by treatment with concanavalin A.", "content": "Weanling BALB/c mice given injections of 300 micrograms concanavalin A (Con A) prior to and at frequent intervals after challenge with Moloney murine sarcoma virus (M-MuSV), 3-methylcholanthrene (MCA), or TEPC-15 plasmacytoma cells showed an enhancement of tumor induction or development. With the M-MuSV and MCA systems, this enhancement was evidenced by larger tumors and, in the MCA system, by more devastating tumors. Regression of the M-MuSV-induced tumors was more prolonged in Con A-treated mice. With the TEPC-15 system, enhancement was evidenced by a more rapid mortality rate in treated animals.", "contents": "Enhancement of experimental tumors in mice by treatment with concanavalin A. Weanling BALB/c mice given injections of 300 micrograms concanavalin A (Con A) prior to and at frequent intervals after challenge with Moloney murine sarcoma virus (M-MuSV), 3-methylcholanthrene (MCA), or TEPC-15 plasmacytoma cells showed an enhancement of tumor induction or development. With the M-MuSV and MCA systems, this enhancement was evidenced by larger tumors and, in the MCA system, by more devastating tumors. Regression of the M-MuSV-induced tumors was more prolonged in Con A-treated mice. With the TEPC-15 system, enhancement was evidenced by a more rapid mortality rate in treated animals."}
{"id": "PMID:480380", "title": "Synergism of diethylstilbestrol and radiation in mammary carcinogenesis in female F344 rats.", "content": "One compressed 20-mg pellet containing cholesterol only or cholesterol mixed with 0.98, 1.6, 2.6, or 3.9 mg of diethylstilbestrol (DES) was implanted into each of 203 female F344 rats. Two days later, half the animals in each group were exposed to 150 R of X-rays, and the other half were sham irradiated. The rats were maintained until 350 days post implantation. Mortality increased with the higher doses of DES, with or without X-rays. DES at all dose levels, with or without X-rays, produced pituitary tumors and pyometritis. Only rats that received both DES and X-rays had mammary adenocarcinomas (AC). A synergistic AC response was found in the group that received 2.6 mg DES plus X-rays. Synergism was defined as a significantly greater incidence of rats with mammary neoplasia resulting from DES plus X-ray treatment when compared to the summed incidence from comparable individual treatments. For all other groups of rats that received both treatments, synergism was detected only when their data were combined. Synergism was not detected among rats that had fibroadenomas (FA). Both types of neoplasms were independent phenomena because no significant relationship was found between the incidences of FA and AC.", "contents": "Synergism of diethylstilbestrol and radiation in mammary carcinogenesis in female F344 rats. One compressed 20-mg pellet containing cholesterol only or cholesterol mixed with 0.98, 1.6, 2.6, or 3.9 mg of diethylstilbestrol (DES) was implanted into each of 203 female F344 rats. Two days later, half the animals in each group were exposed to 150 R of X-rays, and the other half were sham irradiated. The rats were maintained until 350 days post implantation. Mortality increased with the higher doses of DES, with or without X-rays. DES at all dose levels, with or without X-rays, produced pituitary tumors and pyometritis. Only rats that received both DES and X-rays had mammary adenocarcinomas (AC). A synergistic AC response was found in the group that received 2.6 mg DES plus X-rays. Synergism was defined as a significantly greater incidence of rats with mammary neoplasia resulting from DES plus X-ray treatment when compared to the summed incidence from comparable individual treatments. For all other groups of rats that received both treatments, synergism was detected only when their data were combined. Synergism was not detected among rats that had fibroadenomas (FA). Both types of neoplasms were independent phenomena because no significant relationship was found between the incidences of FA and AC."}
{"id": "PMID:480383", "title": "Treatment of young investigators in the National Cancer Program.", "content": "To ascertain whether young investigators (those 35 years of age and under) receive equitable treatment in the National Cancer Institute's (NCI) peer review process, we analyzed recommendation and award rates for new traditional grant applicants for 3 fiscal years (nine cycles of review). The more than 4,500 applications studied showed markedly higher recommendation and award rates for younger investigators than for senior scientists; a peak award rate differential of 15% was reached. The study also revealed that young scientists submitted just one-fourth of all the traditional grant applications sent to NCI and received less than one-third of the funds awarded. These results were consistent throughout the National Institutes of Health. As a follow-up, survey results are reported that assess researchers' attitudes and perceptions of the young scientist's role in the current biomedical review system.", "contents": "Treatment of young investigators in the National Cancer Program. To ascertain whether young investigators (those 35 years of age and under) receive equitable treatment in the National Cancer Institute's (NCI) peer review process, we analyzed recommendation and award rates for new traditional grant applicants for 3 fiscal years (nine cycles of review). The more than 4,500 applications studied showed markedly higher recommendation and award rates for younger investigators than for senior scientists; a peak award rate differential of 15% was reached. The study also revealed that young scientists submitted just one-fourth of all the traditional grant applications sent to NCI and received less than one-third of the funds awarded. These results were consistent throughout the National Institutes of Health. As a follow-up, survey results are reported that assess researchers' attitudes and perceptions of the young scientist's role in the current biomedical review system."}
{"id": "PMID:480382", "title": "Promotional effect of sodium barbiturate on intestinal tumors induced in rats by dimethylhydrazine.", "content": "Noninbred Sprague-Dawley rats were administered 1,2-dimethylhydrazine hydrochloride (DMH) by gavage or methylazoxymethanol acetate by sc inoculation. Thereafter, one group was given water to which 0.1% sodium barbiturate was added, and the other was given drug-free water. They were examined 20 weeks after onset of the experiments. A direct relationship was noted between dosage of DMH and numbers of intestinal tumors induced in the rats. The rats provided with sodium barbiturate-supplemented water developed more intestinal tumors than did those that drank drug-free water.", "contents": "Promotional effect of sodium barbiturate on intestinal tumors induced in rats by dimethylhydrazine. Noninbred Sprague-Dawley rats were administered 1,2-dimethylhydrazine hydrochloride (DMH) by gavage or methylazoxymethanol acetate by sc inoculation. Thereafter, one group was given water to which 0.1% sodium barbiturate was added, and the other was given drug-free water. They were examined 20 weeks after onset of the experiments. A direct relationship was noted between dosage of DMH and numbers of intestinal tumors induced in the rats. The rats provided with sodium barbiturate-supplemented water developed more intestinal tumors than did those that drank drug-free water."}
{"id": "PMID:480384", "title": "Human colon adenocarcinoma cells. III. In vitro organoid expression and carcinoembryonic antigen kinetics in hollow fiber culture.", "content": "Cell line LS174T was established in our laboratory from a primary human colon adenocarcinoma and was serially cultured for 4 years. Following 1 month of culture in perfused hollow fiber matrices, organoid growth reminiscent of the patient's original tumor was observed. The cellular organization was predominantly glandular with mucin located within gland lumina and cells. Glands had irregular brush borders, well-developed junctional complexes, and intracytoplasmic lumina lined with microvilli, features found in adenocarcinoma tissue. Alternate-day determinations of carcinoembryonic antigen (CEA) revealed biphasic kinetics in the extracapillary fluids of the hollow fiber system but not in identically treated monolayer cultures. The initial rate of CEA release was similar to that of monolayer cultures. A significantly accelerated secondary CEA release phase was observed in 10,000- and 50,000-molecular weight (MW)-exclusion fiber cultures (P less than or equal to 0.001 and P = 0.05, respectively). Totals of 43.8 micrograms and 112.6 micrograms CEA were released into the extracapillary fluids of 10,000- and 50,000-MW-exclusion fiber cultures, respectively, which were onefold and 2.3-fold increases, respectively, over monolayer supernatant yields. Most CEA was released during the final days of the secondary phase. In monolayer cultures, maximum CEA release occurred during the stationary phase of growth.", "contents": "Human colon adenocarcinoma cells. III. In vitro organoid expression and carcinoembryonic antigen kinetics in hollow fiber culture. Cell line LS174T was established in our laboratory from a primary human colon adenocarcinoma and was serially cultured for 4 years. Following 1 month of culture in perfused hollow fiber matrices, organoid growth reminiscent of the patient's original tumor was observed. The cellular organization was predominantly glandular with mucin located within gland lumina and cells. Glands had irregular brush borders, well-developed junctional complexes, and intracytoplasmic lumina lined with microvilli, features found in adenocarcinoma tissue. Alternate-day determinations of carcinoembryonic antigen (CEA) revealed biphasic kinetics in the extracapillary fluids of the hollow fiber system but not in identically treated monolayer cultures. The initial rate of CEA release was similar to that of monolayer cultures. A significantly accelerated secondary CEA release phase was observed in 10,000- and 50,000-molecular weight (MW)-exclusion fiber cultures (P less than or equal to 0.001 and P = 0.05, respectively). Totals of 43.8 micrograms and 112.6 micrograms CEA were released into the extracapillary fluids of 10,000- and 50,000-MW-exclusion fiber cultures, respectively, which were onefold and 2.3-fold increases, respectively, over monolayer supernatant yields. Most CEA was released during the final days of the secondary phase. In monolayer cultures, maximum CEA release occurred during the stationary phase of growth."}
{"id": "PMID:480385", "title": "Hereditary retinoblastoma: host resistance and age at onset.", "content": "Age-specific incidence data from 244 bilateral and 31 familial unlateral cases of retinoblastoma were analyzed. In the bilateral cases, a high intraclass correlation between ages of the patients at diagnosis in the right and left eyes was noted, even after removal of the bias due to the fact that the diagnosis for each eye is never independent. This finding indicated strongly that the age at onset was largely determined by host factors common to both eyes. The mean ages of the patients at diagnosis of the inherited cases among the bilateral or unilateral group varied consistently with parental phenotype. This finding implied that inherited host resistance played an important role in the latency period for the gene carriers. The fraction of cases not yet diagnosed fit well to a negative exponential distribution, and in the less susceptible group with hereditary unilateral cases, tumor formation may have been suppressed about twice that in the most susceptible group with bilateral cases. It was argued that the presumed second hit initiating tumor formation in the gene carriers was not a mutational process, but probably an error in the process of differentiation that could be suppressed completely in the most resistant group who could remain unaffected, but that could not be suppressed completely in the less resistant group.", "contents": "Hereditary retinoblastoma: host resistance and age at onset. Age-specific incidence data from 244 bilateral and 31 familial unlateral cases of retinoblastoma were analyzed. In the bilateral cases, a high intraclass correlation between ages of the patients at diagnosis in the right and left eyes was noted, even after removal of the bias due to the fact that the diagnosis for each eye is never independent. This finding indicated strongly that the age at onset was largely determined by host factors common to both eyes. The mean ages of the patients at diagnosis of the inherited cases among the bilateral or unilateral group varied consistently with parental phenotype. This finding implied that inherited host resistance played an important role in the latency period for the gene carriers. The fraction of cases not yet diagnosed fit well to a negative exponential distribution, and in the less susceptible group with hereditary unilateral cases, tumor formation may have been suppressed about twice that in the most susceptible group with bilateral cases. It was argued that the presumed second hit initiating tumor formation in the gene carriers was not a mutational process, but probably an error in the process of differentiation that could be suppressed completely in the most resistant group who could remain unaffected, but that could not be suppressed completely in the less resistant group."}
{"id": "PMID:480386", "title": "Case-control study of hair dye use by patients with breast cancer and endometrial cancer.", "content": "A case-control study was undertaken of use of permanent and semipermanent hair dyes by women with cancers of several sites, including breast and endometrium. In London, Ontario, 50 cases of of breast cancer and in Toronto 35 cases of breast cancer and 36 cases of endometrial cancer were identified in cancer treatment centers. In London, controls were selected from hospitalized women with diseases other than cancer; in Toronto, controls were selected from women living in the same neighborhood as the patients with cancer. The results did not suggest an increased risk of either breast or endometrial cancer in users of permanent or permanent and semipermanent dyes combined. Although the numbers of cases and controls were small, the consistency of the results for both sites, in both study centers, and the absence of any clear positive relationship between various measures of intensity of use and risk of cancer provided evidence that a large increase in risk was not missed.", "contents": "Case-control study of hair dye use by patients with breast cancer and endometrial cancer. A case-control study was undertaken of use of permanent and semipermanent hair dyes by women with cancers of several sites, including breast and endometrium. In London, Ontario, 50 cases of of breast cancer and in Toronto 35 cases of breast cancer and 36 cases of endometrial cancer were identified in cancer treatment centers. In London, controls were selected from hospitalized women with diseases other than cancer; in Toronto, controls were selected from women living in the same neighborhood as the patients with cancer. The results did not suggest an increased risk of either breast or endometrial cancer in users of permanent or permanent and semipermanent dyes combined. Although the numbers of cases and controls were small, the consistency of the results for both sites, in both study centers, and the absence of any clear positive relationship between various measures of intensity of use and risk of cancer provided evidence that a large increase in risk was not missed."}
{"id": "PMID:480387", "title": "Intake of volatile nitrosamines from consumption of alcohols.", "content": "Volatile nitrosamines were determined in alcoholic drinks during epidemiologic studies on the relationship between esophageal cancer incidence and alcohol consumption in Normandy, France. Nitrosodimethylamine (NDMA) was found commonly in most alcoholic drinks tested, with the exception of wine. The average level, about 2 micrograms/liter in beers, was higher than that for other drinks; the range was 0.2--8.6 micrograms/liter. Traces of nitrosodiethylamine (NDEA) were also detected in spirits and ciders. No significant increases in levels were found after nitrosation. Calculation of daily intake in the study region showed that the main intake of volatile nitrosamine is from NDMA in beer. The intake of NDEA through consumption of cider is about one-third that of NDMA from all sources.", "contents": "Intake of volatile nitrosamines from consumption of alcohols. Volatile nitrosamines were determined in alcoholic drinks during epidemiologic studies on the relationship between esophageal cancer incidence and alcohol consumption in Normandy, France. Nitrosodimethylamine (NDMA) was found commonly in most alcoholic drinks tested, with the exception of wine. The average level, about 2 micrograms/liter in beers, was higher than that for other drinks; the range was 0.2--8.6 micrograms/liter. Traces of nitrosodiethylamine (NDEA) were also detected in spirits and ciders. No significant increases in levels were found after nitrosation. Calculation of daily intake in the study region showed that the main intake of volatile nitrosamine is from NDMA in beer. The intake of NDEA through consumption of cider is about one-third that of NDMA from all sources."}
{"id": "PMID:480388", "title": "Premalignant lesions of the cervix in women of Cali, Colombia.", "content": "A detailed histologic study was made of uterine cervices from 441 autopsy specimens from the population of Cali, Colombia. Women in this city have one of the highest registered incidence rates of cancer of the uterine cervix. The following prevalence (in percent) of lesions in adult women was found: cervicitis, 79; reserve cell hyperplasia, 14; squamous metaplasia, 41; dysplasia, 9. Contrary to expectations, no increase in prevalence of these lesions was found with age, lower socioeconomic status, or number of pregnancies. A similar study of a sample of hysterectomies performed for uterine prolapse showed approximately the same results. The findings suggest that promotional factors are more important than initiator factors in uterine cervical carcinogenesis.", "contents": "Premalignant lesions of the cervix in women of Cali, Colombia. A detailed histologic study was made of uterine cervices from 441 autopsy specimens from the population of Cali, Colombia. Women in this city have one of the highest registered incidence rates of cancer of the uterine cervix. The following prevalence (in percent) of lesions in adult women was found: cervicitis, 79; reserve cell hyperplasia, 14; squamous metaplasia, 41; dysplasia, 9. Contrary to expectations, no increase in prevalence of these lesions was found with age, lower socioeconomic status, or number of pregnancies. A similar study of a sample of hysterectomies performed for uterine prolapse showed approximately the same results. The findings suggest that promotional factors are more important than initiator factors in uterine cervical carcinogenesis."}
{"id": "PMID:480389", "title": "Development of Leydig cell tumors and onset of changes in the reproductive and endocrine systems of aging F344 rats.", "content": "The age-dependent onset of spontaneous testicular interstitial cell tumors was examined in F344 male rats. Light microscopy of testes established that nodular interstitial cell hyperplasia was evident in 3 of 5 12-month-old rats and in 5 of 5 rats at 15, 18, 21, and 24 months of age. Involution of the seminiferous epithelium was evident in all testes exhibiting extensive interstitial cell proliferation. Striking increments in serum prolactin and estradiol levels were noted with advancing age, whereas serum levels of follicle-stimulating hormone were unequivocally lower at 21 and 24 months than at 6 months of age. No measurable changes were detected in serum testosterone concentrations between 6 and 18 months of age, but marked increments in this androgen, without any measurable change in circulating luteinizing hormone titers, were apparent in 21- and 24-month-old rats. These findings point to a dynamic relationship between testicular interstitial cell tumorigenesis and age-related changes in the synthesis and/or secretion of gonadal and adenohypophyseal hormones.", "contents": "Development of Leydig cell tumors and onset of changes in the reproductive and endocrine systems of aging F344 rats. The age-dependent onset of spontaneous testicular interstitial cell tumors was examined in F344 male rats. Light microscopy of testes established that nodular interstitial cell hyperplasia was evident in 3 of 5 12-month-old rats and in 5 of 5 rats at 15, 18, 21, and 24 months of age. Involution of the seminiferous epithelium was evident in all testes exhibiting extensive interstitial cell proliferation. Striking increments in serum prolactin and estradiol levels were noted with advancing age, whereas serum levels of follicle-stimulating hormone were unequivocally lower at 21 and 24 months than at 6 months of age. No measurable changes were detected in serum testosterone concentrations between 6 and 18 months of age, but marked increments in this androgen, without any measurable change in circulating luteinizing hormone titers, were apparent in 21- and 24-month-old rats. These findings point to a dynamic relationship between testicular interstitial cell tumorigenesis and age-related changes in the synthesis and/or secretion of gonadal and adenohypophyseal hormones."}
{"id": "PMID:480390", "title": "Induction of tumors by direct injection of N-ethyl-N-nitrosourea into the amniotic space of the mouse fetus.", "content": "An N-ethyl-N-nitrosourea (ENU) solution was injected into the amniotic space of Swiss mouse fetuses. Embryos 9 days old were exposed to 2 and 4 micrograms ENU and died within the first 4 weeks of life. Injection of 4 micrograms ENU into 11-day-old fetuses induced a 10% lung tumor incidence, whereas 16 micrograms injected at 12, 15, or 16 days resulted in lung tumor incidences of 42, 100, and 84%, respectively. A mortality rate of 50% and a lung tumor incidence of 89% in survivors resulted after exposure of 16-day-old fetuses to 64 micrograms ENU. Histologic examination revealed that the tumors were alveologenic adenomas 1--7 mm in diameter. Two saline-treated control groups had no tumors. The possible utilization of this biologic model in testing chemical alkylating agents similar to ENU is discussed.", "contents": "Induction of tumors by direct injection of N-ethyl-N-nitrosourea into the amniotic space of the mouse fetus. An N-ethyl-N-nitrosourea (ENU) solution was injected into the amniotic space of Swiss mouse fetuses. Embryos 9 days old were exposed to 2 and 4 micrograms ENU and died within the first 4 weeks of life. Injection of 4 micrograms ENU into 11-day-old fetuses induced a 10% lung tumor incidence, whereas 16 micrograms injected at 12, 15, or 16 days resulted in lung tumor incidences of 42, 100, and 84%, respectively. A mortality rate of 50% and a lung tumor incidence of 89% in survivors resulted after exposure of 16-day-old fetuses to 64 micrograms ENU. Histologic examination revealed that the tumors were alveologenic adenomas 1--7 mm in diameter. Two saline-treated control groups had no tumors. The possible utilization of this biologic model in testing chemical alkylating agents similar to ENU is discussed."}
{"id": "PMID:480392", "title": "Histoplasmosis: clinical syndromes and management.", "content": "The fungus Histoplasma capsulatum produces a spectrum of disease forms ranging from a benign self-limited illness to progressive disseminated disease with a 50 percent mortality rate. The drug of choice, amphotericin B, must be given intravenously over a prolonged course and carries a high incidence of toxicity. Thus, optimal managment of serious forms of histoplasmosis requires considerable clinical judgment.", "contents": "Histoplasmosis: clinical syndromes and management. The fungus Histoplasma capsulatum produces a spectrum of disease forms ranging from a benign self-limited illness to progressive disseminated disease with a 50 percent mortality rate. The drug of choice, amphotericin B, must be given intravenously over a prolonged course and carries a high incidence of toxicity. Thus, optimal managment of serious forms of histoplasmosis requires considerable clinical judgment."}
{"id": "PMID:480393", "title": "Analysis of the medical records in a pediatric emergency room.", "content": "A prospective evaluation of Pediatric Emergency Room records permitted analysis of major errors and of factors contributing to them. All records from July 1973 to June 1975 were reviewed daily by a board certified pediatrician using a previously established protocol. Patients had been seen by pediatric house staff from 4 PM to 8 AM on weekdays and from 8 AM to 8 AM on weekends and 25,907 records were reviewed. Errors were detected in 9.5 percent of these. The most common was an incomplete set of vital signs which accounts for 68 percent of all errors. Failure to arrange for appropriate follow-up care occurred in 16 percent of cases. Other errors ranged from two to four percent and included inadequate use of laboratory, incomplete physical examination, inappropriate diagnosis or therapy. Major omissions in the history were uncommon (1.3 percent). The frequency of errors was significantly greater at the start of each academic year (July to October), and at the start of each month (P<0.001). The errors occurred significantly more often at the first year level than the second year level (P<0.01). This study suggests a means of improving record keeping and house staff education, ie, attending-level supervision should be emphasized at the start of each month and academic year. Daily reviews of errors with the house staff as well as modification of chart design may bring about more complete patient evaluation and detailed recording of findings, diagnosis, and disposition.", "contents": "Analysis of the medical records in a pediatric emergency room. A prospective evaluation of Pediatric Emergency Room records permitted analysis of major errors and of factors contributing to them. All records from July 1973 to June 1975 were reviewed daily by a board certified pediatrician using a previously established protocol. Patients had been seen by pediatric house staff from 4 PM to 8 AM on weekdays and from 8 AM to 8 AM on weekends and 25,907 records were reviewed. Errors were detected in 9.5 percent of these. The most common was an incomplete set of vital signs which accounts for 68 percent of all errors. Failure to arrange for appropriate follow-up care occurred in 16 percent of cases. Other errors ranged from two to four percent and included inadequate use of laboratory, incomplete physical examination, inappropriate diagnosis or therapy. Major omissions in the history were uncommon (1.3 percent). The frequency of errors was significantly greater at the start of each academic year (July to October), and at the start of each month (P<0.001). The errors occurred significantly more often at the first year level than the second year level (P<0.01). This study suggests a means of improving record keeping and house staff education, ie, attending-level supervision should be emphasized at the start of each month and academic year. Daily reviews of errors with the house staff as well as modification of chart design may bring about more complete patient evaluation and detailed recording of findings, diagnosis, and disposition."}
{"id": "PMID:480394", "title": "A case of ovarian pregnancy.", "content": "Ovarian pregnancy is an uncommon event, the cause of which is unknown. The use of the intrauterine device (IUD) has increased the incidence of ovarian pregnancy. This is due to the decreasing contraceptive effect of the IUD on the fallopian tube and the ovary. This case satisfies the four criteria of Spiegelberg. It also demonstrates the advantages of laparoscopy and ultrasonography in patients with obscure pelvic pain.", "contents": "A case of ovarian pregnancy. Ovarian pregnancy is an uncommon event, the cause of which is unknown. The use of the intrauterine device (IUD) has increased the incidence of ovarian pregnancy. This is due to the decreasing contraceptive effect of the IUD on the fallopian tube and the ovary. This case satisfies the four criteria of Spiegelberg. It also demonstrates the advantages of laparoscopy and ultrasonography in patients with obscure pelvic pain."}
{"id": "PMID:480395", "title": "Primary malignant melanoma of the bronchus.", "content": "A case is presented of a 55-year-old woman with fever, cough, chest pain, and hemoptysis. A chest x-ray showed a large mass in the right upper lobe of the lung. Bronchoscopy and bronchial biopsies revealed malignant melanoma of the bronchus. Careful search of all common sites for melanoma and the histological examinations of the two skin lesions failed to substantiate the possibility of an extrapulmonary origin and, thus, by deduction it can be assumed with some certainty that this lesion is primary bronchial melanoma.Total pneumonectomy, when there is no evidence of extrapulmonary extension, coupled with adjuvant chemotherapy seem to offer a chance of cure.The patient presented is the first reported case of primary malignant melanoma of the bronchus from the University College Hospital, Ibadan, and perhaps the first reported case in a Nigerian.", "contents": "Primary malignant melanoma of the bronchus. A case is presented of a 55-year-old woman with fever, cough, chest pain, and hemoptysis. A chest x-ray showed a large mass in the right upper lobe of the lung. Bronchoscopy and bronchial biopsies revealed malignant melanoma of the bronchus. Careful search of all common sites for melanoma and the histological examinations of the two skin lesions failed to substantiate the possibility of an extrapulmonary origin and, thus, by deduction it can be assumed with some certainty that this lesion is primary bronchial melanoma.Total pneumonectomy, when there is no evidence of extrapulmonary extension, coupled with adjuvant chemotherapy seem to offer a chance of cure.The patient presented is the first reported case of primary malignant melanoma of the bronchus from the University College Hospital, Ibadan, and perhaps the first reported case in a Nigerian."}
{"id": "PMID:480396", "title": "Pattern of hemoglobin concentration in untreated Burkitt lymphoma patients in Ibadan.", "content": "Hemoglobin concentration is studied in 128 untreated Burkitt lymphoma patients in Ibadan. While there is no significant diagnostic pattern in hemoglobin concentration, Burkitt lymphoma patients show marked anemia as compared with healthy adult Nigerians. The degree of involvement of malarial parasitemia in producing anemia in Burkitt lymphoma patients has yet to be determined.", "contents": "Pattern of hemoglobin concentration in untreated Burkitt lymphoma patients in Ibadan. Hemoglobin concentration is studied in 128 untreated Burkitt lymphoma patients in Ibadan. While there is no significant diagnostic pattern in hemoglobin concentration, Burkitt lymphoma patients show marked anemia as compared with healthy adult Nigerians. The degree of involvement of malarial parasitemia in producing anemia in Burkitt lymphoma patients has yet to be determined."}
{"id": "PMID:480397", "title": "Acute management of the sexual assault victim.", "content": "Sexual assault victims usually seek emergency medical care at urban hospitals. The incidence of over 52,000 cases a year reported by the FBI does not include instances in which the victim died, cases of statutory rape (victim below the age of consent), or cases not confirmed by the police. It is well known that sexual assault is one of the most unreported crimes. The incidence of this crime in affluent communities is unknown. Rape occurs in any community but nonhospital professional services may be more easily available in some.The office practitioner may feel ill-equipped to deal with this kind of problem because such patients seldom come to a private office; although in a survey done in 1976, 33 percent of family practitioners stated that they see several rape victims each year. (1) The practitioner may be hesitant about getting involved in litigation. Experience indicates that by following the approach described and documenting facts clearly, physicians will prevent disruption of their busy schedules by unfamiliar court routines, and at the same time provide what society, the victim, and the alleged assailant expect of him as a member of the health care team.", "contents": "Acute management of the sexual assault victim. Sexual assault victims usually seek emergency medical care at urban hospitals. The incidence of over 52,000 cases a year reported by the FBI does not include instances in which the victim died, cases of statutory rape (victim below the age of consent), or cases not confirmed by the police. It is well known that sexual assault is one of the most unreported crimes. The incidence of this crime in affluent communities is unknown. Rape occurs in any community but nonhospital professional services may be more easily available in some.The office practitioner may feel ill-equipped to deal with this kind of problem because such patients seldom come to a private office; although in a survey done in 1976, 33 percent of family practitioners stated that they see several rape victims each year. (1) The practitioner may be hesitant about getting involved in litigation. Experience indicates that by following the approach described and documenting facts clearly, physicians will prevent disruption of their busy schedules by unfamiliar court routines, and at the same time provide what society, the victim, and the alleged assailant expect of him as a member of the health care team."}
{"id": "PMID:480398", "title": "Eugenic sterilization: medico-legal and sociological aspects.", "content": "The court-ordered sterilization of a normal 18-year-old black female in North Carolina focused attention on the unfortunate impact of eugenic statutes that allow sterilization as a method of social control. The existence of these laws in many states allows misapplication and abuse of authority which, not infrequently, is directed liberally to blacks and other minority groups.Eugenic sterilization is, at this time, a legally accepted form of \"medical treatment.\" The justification of such sterilization is the vague concept that the presumed \"mentally deficient\" individual is probably a potential parent of socially inadequate offspring who would likewise be socially inadequate.Since there never has been factual substantiation of whether the sterilization of these individuals will diminish the incidence of mental retardation, it is necessary to focus attention on the concept of eugenic sterilization and point out its many fallacies.", "contents": "Eugenic sterilization: medico-legal and sociological aspects. The court-ordered sterilization of a normal 18-year-old black female in North Carolina focused attention on the unfortunate impact of eugenic statutes that allow sterilization as a method of social control. The existence of these laws in many states allows misapplication and abuse of authority which, not infrequently, is directed liberally to blacks and other minority groups.Eugenic sterilization is, at this time, a legally accepted form of \"medical treatment.\" The justification of such sterilization is the vague concept that the presumed \"mentally deficient\" individual is probably a potential parent of socially inadequate offspring who would likewise be socially inadequate.Since there never has been factual substantiation of whether the sterilization of these individuals will diminish the incidence of mental retardation, it is necessary to focus attention on the concept of eugenic sterilization and point out its many fallacies."}
{"id": "PMID:480399", "title": "Roots: its impact and implications.", "content": "What is contained in Roots, the 587-page narrative that captured an entire world population? The answer is not simple, nor is it overly complex, but rather an admixture of significant psychological, sociological, and timing factors that served to ignite the fuse of human fascination for the unknown, the hidden truths, the obscure, and the forbidden. This paper analyzes the impact and implications of Roots on many facets of American society.", "contents": "Roots: its impact and implications. What is contained in Roots, the 587-page narrative that captured an entire world population? The answer is not simple, nor is it overly complex, but rather an admixture of significant psychological, sociological, and timing factors that served to ignite the fuse of human fascination for the unknown, the hidden truths, the obscure, and the forbidden. This paper analyzes the impact and implications of Roots on many facets of American society."}
{"id": "PMID:480401", "title": "Suppressive effect of dipyridamole on the proteinuria of aminonucleoside nephrosis in rat.", "content": "The suppressive effect of dipyridamole on the proteinuria of aminonucleoside nephrosis and protamine-induced proteinuria, was investigated. Male Wistar rats were given puromycin aminonucleoside (80 mg/kg s.c.) or protamine sulfate (20 mg/kg i.v.), and the urine was collected in metabolic cages. The content of proteins in the urine was determined by using a continuous gradient microgel electrophoresis procedure. Dipyridamole (20 mg/kg p.o.) suppressed the excretion of albumin and proteins larger than albumin (HMP) in aminonucleoside nephrosis. But the excertion of proteins smaller than albumin (LMP) was not affected by dipyridamole. Dipyridamole also suppressed the excertion of HMP in protamine-induced proteinuria, though the excretion of albumin and LMP was not affected. Puromycin aminonucleoside and protamine sulfate were known to cause renal glomerular epithelial changes referred to as \"fusion\" of foot processes. Since dipyridamole was effective in suppressing the both types of proteinuria, this drug was considered to improve the damaged renal glomerular barrier for plasma proteins.", "contents": "Suppressive effect of dipyridamole on the proteinuria of aminonucleoside nephrosis in rat. The suppressive effect of dipyridamole on the proteinuria of aminonucleoside nephrosis and protamine-induced proteinuria, was investigated. Male Wistar rats were given puromycin aminonucleoside (80 mg/kg s.c.) or protamine sulfate (20 mg/kg i.v.), and the urine was collected in metabolic cages. The content of proteins in the urine was determined by using a continuous gradient microgel electrophoresis procedure. Dipyridamole (20 mg/kg p.o.) suppressed the excretion of albumin and proteins larger than albumin (HMP) in aminonucleoside nephrosis. But the excertion of proteins smaller than albumin (LMP) was not affected by dipyridamole. Dipyridamole also suppressed the excertion of HMP in protamine-induced proteinuria, though the excretion of albumin and LMP was not affected. Puromycin aminonucleoside and protamine sulfate were known to cause renal glomerular epithelial changes referred to as \"fusion\" of foot processes. Since dipyridamole was effective in suppressing the both types of proteinuria, this drug was considered to improve the damaged renal glomerular barrier for plasma proteins."}
{"id": "PMID:480402", "title": "Interaction of zinc and other metal on the activity of erythrocyte delta-aminolevulinic acid dehydratase in vitro.", "content": "The interaction of zinc and other metal such as lead, mercury (II), cadmium, silver (II) on the activity of erythrocyte delta-aminolevulinic acid dehydratase (ALA-D) was investigated in vitro. At the concentrations ranging from 0.01 to 1.0 mmol/l, lead, mercury (II), cadmium and silver (II) strikingly inhibited the erythrocyte ALA-D activity. The in vitro addition of zinc having an activating effect for the erythrocyte ALA-D, was observed to eliminate the lead-induced inhibition of the erythrocyte ALA-D activity. And the degree of the elimination seemed to depend on the molar ratio (Zn/Pb) of both metal concentrations in the ALA-D assay. However, the inhibition of erythrocyte ALA-D activity caused by mercury (II), cadmium and silver (II) was not removed by the in vitro addition of zinc.", "contents": "Interaction of zinc and other metal on the activity of erythrocyte delta-aminolevulinic acid dehydratase in vitro. The interaction of zinc and other metal such as lead, mercury (II), cadmium, silver (II) on the activity of erythrocyte delta-aminolevulinic acid dehydratase (ALA-D) was investigated in vitro. At the concentrations ranging from 0.01 to 1.0 mmol/l, lead, mercury (II), cadmium and silver (II) strikingly inhibited the erythrocyte ALA-D activity. The in vitro addition of zinc having an activating effect for the erythrocyte ALA-D, was observed to eliminate the lead-induced inhibition of the erythrocyte ALA-D activity. And the degree of the elimination seemed to depend on the molar ratio (Zn/Pb) of both metal concentrations in the ALA-D assay. However, the inhibition of erythrocyte ALA-D activity caused by mercury (II), cadmium and silver (II) was not removed by the in vitro addition of zinc."}
{"id": "PMID:480403", "title": "Effect of aflatoxin B1 on the ultrastructural and biochemical development of chick embryo liver during organ culture.", "content": "When liver fragments from eleven-day chick embryos were maintained on Eagle's minimal essential medium by the established method of organ culture, they developed ultrastructural features similar to liver cells in vivo, except that they had small amounts of smooth endoplasmic reticulum and little glycogen. The cultured liver cells synthesized DNA, RNA and protein. The addition of aflatoxin B1 to the medium inhibited the synthesis of nucleic acid. Aflatoxin B1 also produced the segregation of granular and fibrillar components in nucleoli and the disarrangement of ribosomes attached to endoplasmic reticulum. Since these results were consistent with the known effects of the toxin in animals, we concluded that organ culture of chick embryo liver could be a useful technique for other studies.", "contents": "Effect of aflatoxin B1 on the ultrastructural and biochemical development of chick embryo liver during organ culture. When liver fragments from eleven-day chick embryos were maintained on Eagle's minimal essential medium by the established method of organ culture, they developed ultrastructural features similar to liver cells in vivo, except that they had small amounts of smooth endoplasmic reticulum and little glycogen. The cultured liver cells synthesized DNA, RNA and protein. The addition of aflatoxin B1 to the medium inhibited the synthesis of nucleic acid. Aflatoxin B1 also produced the segregation of granular and fibrillar components in nucleoli and the disarrangement of ribosomes attached to endoplasmic reticulum. Since these results were consistent with the known effects of the toxin in animals, we concluded that organ culture of chick embryo liver could be a useful technique for other studies."}
{"id": "PMID:480404", "title": "Histopathological study on effects of potassium aspartate on the hypothalamus of rats.", "content": "Rats of various ages were treated orally or intraperitoneally with potassium aspartate. The dose required to induce hypothalamic lesion varied considerably by the age of animals and route of administration. Additional experiment, in which the animals were orally treated three times a day with potassium aspartate in dose levels between the maximum safety dose and minimum lesion-producing dose in the preceding single dose study, revealed no hypothalamic lesion at all in any animals of each age group. In this condition, the maximum safety dose was 3--5 times as large as that in single dosage administration experiment. Regarding the safety evaluation of potassium aspartate preparations, brief discussions on some points in extrapolation of the results of the present experimental study to the clinical use were made.", "contents": "Histopathological study on effects of potassium aspartate on the hypothalamus of rats. Rats of various ages were treated orally or intraperitoneally with potassium aspartate. The dose required to induce hypothalamic lesion varied considerably by the age of animals and route of administration. Additional experiment, in which the animals were orally treated three times a day with potassium aspartate in dose levels between the maximum safety dose and minimum lesion-producing dose in the preceding single dose study, revealed no hypothalamic lesion at all in any animals of each age group. In this condition, the maximum safety dose was 3--5 times as large as that in single dosage administration experiment. Regarding the safety evaluation of potassium aspartate preparations, brief discussions on some points in extrapolation of the results of the present experimental study to the clinical use were made."}
{"id": "PMID:480405", "title": "Reproduction study of carteolol hydrochloride in mice. Part I. Fertility and reproductive performance.", "content": "Carteolol was administered orally by gastric intubation at 3, 15, 75 or 150 mg/kg/day to ICR-JCL mice of both sexes prior to mating and to females during early stage of pregnancy to determine its effects on the entire reproductive process and fetal development. Following results were obtained: 1) The decrease of spontaneous motor activity was observed in all treatment groups, and some animals in the 75 mg/kg and 150 mg/kg groups pressed the chest or abdomen against the cage wall. 2) The incidence of early resorptions in the 75 mg/kg and 150 mg/kg groups was significantly higher than that in controls.3) The ossification of the talus and calcaneus was significantly retarded in the 75 mg/kg and 150 mg/kg groups. The maximum non-effective dose on fertility and reproduction for carteolol was estimated to be 15 mg/kg/day, although the incidence of early resorptions was slightly elevated with this dose.", "contents": "Reproduction study of carteolol hydrochloride in mice. Part I. Fertility and reproductive performance. Carteolol was administered orally by gastric intubation at 3, 15, 75 or 150 mg/kg/day to ICR-JCL mice of both sexes prior to mating and to females during early stage of pregnancy to determine its effects on the entire reproductive process and fetal development. Following results were obtained: 1) The decrease of spontaneous motor activity was observed in all treatment groups, and some animals in the 75 mg/kg and 150 mg/kg groups pressed the chest or abdomen against the cage wall. 2) The incidence of early resorptions in the 75 mg/kg and 150 mg/kg groups was significantly higher than that in controls.3) The ossification of the talus and calcaneus was significantly retarded in the 75 mg/kg and 150 mg/kg groups. The maximum non-effective dose on fertility and reproduction for carteolol was estimated to be 15 mg/kg/day, although the incidence of early resorptions was slightly elevated with this dose."}
{"id": "PMID:480406", "title": "Reproduction study of carteolol hydrochloride in mice. Part 2. Peri -and postnatal toxicity.", "content": "Carteolol was orally administered to mice once a day at doses of 3, 30 and 150 mg/kg/day during the perinatal and lactation periods and evaluated on its adverse effects on pregnant animals and their offspring. No appreciably abnormal findings related to the drug administration were revealed. Therefore, it was concluded that carteolol have no serious toxic potential on parturition and lactation by mother animals, no adverse effects on growth and development, and behavioral and reproductive performance of offspring and no carcinogenic action through placental and milk transfer.", "contents": "Reproduction study of carteolol hydrochloride in mice. Part 2. Peri -and postnatal toxicity. Carteolol was orally administered to mice once a day at doses of 3, 30 and 150 mg/kg/day during the perinatal and lactation periods and evaluated on its adverse effects on pregnant animals and their offspring. No appreciably abnormal findings related to the drug administration were revealed. Therefore, it was concluded that carteolol have no serious toxic potential on parturition and lactation by mother animals, no adverse effects on growth and development, and behavioral and reproductive performance of offspring and no carcinogenic action through placental and milk transfer."}
{"id": "PMID:480413", "title": "[Ectopic ureter ending in the seminal vesicle in adults. Apropos of a case and review of the literature].", "content": "The authors report a 38 years old patient in whom an intravenous pyelogram was performed for moderate arterial hypertension. This showed a large filling defect in the right lower part of the bladder without renal function on that side. Endoscopic separation of the jet coming from the right half of the trigone showed a liquid containing numerous spermatozoa. A cystogram opacified by reflux a cavity in the genital system via this, the right ureter. With the diagnosis of ectopic ureter ending in the genital system, a nephro-ureterectomy demonstrated the attachment of the ureter into a pouch which communicated with the bladder and the seminal vesicle. A view of the normal embryology insists on the fact that contrary to the classic view, the pronephros disappears entirely and that the metanephros is at the origin of the Woffian canal which opens on the posterior wall of the urogenital sinus at the 28th day when the ureter springs from a ureteral bud. Since 1960 this would be the 34th case in the literature, seminal ectopia (24%) coming after ectopia in the prostatic urethra (54%). Epididymitis is a frequent presenting symptom (44%). The workup shows: absence of renal function (100%), raising of the ipsilateral trigone by a cystic swelling (76%), absence of the meatus on that side on endoscopy, presence of an ipsilateral mass above the prostate on rectal examination (30%). The diagnosis is confirmed by vasography where the ureter is opacified by the seminal vesicle or by systography after endoscopic incision of the mass. Excision of the seminal vesicle was done in conjunction with total nephroureterectomy in 17% of cases.", "contents": "[Ectopic ureter ending in the seminal vesicle in adults. Apropos of a case and review of the literature]. The authors report a 38 years old patient in whom an intravenous pyelogram was performed for moderate arterial hypertension. This showed a large filling defect in the right lower part of the bladder without renal function on that side. Endoscopic separation of the jet coming from the right half of the trigone showed a liquid containing numerous spermatozoa. A cystogram opacified by reflux a cavity in the genital system via this, the right ureter. With the diagnosis of ectopic ureter ending in the genital system, a nephro-ureterectomy demonstrated the attachment of the ureter into a pouch which communicated with the bladder and the seminal vesicle. A view of the normal embryology insists on the fact that contrary to the classic view, the pronephros disappears entirely and that the metanephros is at the origin of the Woffian canal which opens on the posterior wall of the urogenital sinus at the 28th day when the ureter springs from a ureteral bud. Since 1960 this would be the 34th case in the literature, seminal ectopia (24%) coming after ectopia in the prostatic urethra (54%). Epididymitis is a frequent presenting symptom (44%). The workup shows: absence of renal function (100%), raising of the ipsilateral trigone by a cystic swelling (76%), absence of the meatus on that side on endoscopy, presence of an ipsilateral mass above the prostate on rectal examination (30%). The diagnosis is confirmed by vasography where the ureter is opacified by the seminal vesicle or by systography after endoscopic incision of the mass. Excision of the seminal vesicle was done in conjunction with total nephroureterectomy in 17% of cases."}
{"id": "PMID:480415", "title": "[Surgical treatment of bladder exstrophy by functional reconstruction by stages (author's transl)].", "content": "A brief summary is given of the clinical statistical findings in 37 patients with exstrophy of the bladder, most of the whom have been reported in a previous paper. Four cases are presented for whom the method of operating by stages was applied. They have now been followed up for 17, 10, 8 and 6 years respectively and a very satisfactory functional result was obtained. The experience gained from the use of such a method clearly indicates that treatment should be carried out, whenever possible, by a one-stage operation, or by two stages within a short period of time, and at an early age. Two cases operated upon by this latter method of total primary functional reconstruction, with very encouraging morphological results, are reported.", "contents": "[Surgical treatment of bladder exstrophy by functional reconstruction by stages (author's transl)]. A brief summary is given of the clinical statistical findings in 37 patients with exstrophy of the bladder, most of the whom have been reported in a previous paper. Four cases are presented for whom the method of operating by stages was applied. They have now been followed up for 17, 10, 8 and 6 years respectively and a very satisfactory functional result was obtained. The experience gained from the use of such a method clearly indicates that treatment should be carried out, whenever possible, by a one-stage operation, or by two stages within a short period of time, and at an early age. Two cases operated upon by this latter method of total primary functional reconstruction, with very encouraging morphological results, are reported."}
{"id": "PMID:480417", "title": "[Replacement of the lower third of the ureter via the bladder (author's transl)].", "content": "The author suggests a special incision in the bladder permitting the formation of a tube using a strip of bladder long enough to permit an anti-reflux vesico ureteral reimplantation. The method is a combination of the Boari and the transverse bipartition described by Turner Warwick. Extraperitonealization of the hemi-bladder opposite to the diseased ureter and section of the umbilical artery. Transverse anterior cystostomy at mid height of the bladder. The mobilized dome of the bladder is stitched up to the psoas muscle above the iliac vessels. Creation of a rectangular flat, the anterior limit of which is the superior margin of the transverse cystostomy, of which the upper part is a split which cuts the dome of the bladder from front to back, of which the posterior limit is a split on the posterior wall of the bladder, parallel to the anterior incision. The length must not be larger than one and one half the width of the flap. Reimplantation of the ureter, creation of the tube and closure of the bladder is done without difficulty. Drawings illustrate the technic. Four cases are reported with three successes.", "contents": "[Replacement of the lower third of the ureter via the bladder (author's transl)]. The author suggests a special incision in the bladder permitting the formation of a tube using a strip of bladder long enough to permit an anti-reflux vesico ureteral reimplantation. The method is a combination of the Boari and the transverse bipartition described by Turner Warwick. Extraperitonealization of the hemi-bladder opposite to the diseased ureter and section of the umbilical artery. Transverse anterior cystostomy at mid height of the bladder. The mobilized dome of the bladder is stitched up to the psoas muscle above the iliac vessels. Creation of a rectangular flat, the anterior limit of which is the superior margin of the transverse cystostomy, of which the upper part is a split which cuts the dome of the bladder from front to back, of which the posterior limit is a split on the posterior wall of the bladder, parallel to the anterior incision. The length must not be larger than one and one half the width of the flap. Reimplantation of the ureter, creation of the tube and closure of the bladder is done without difficulty. Drawings illustrate the technic. Four cases are reported with three successes."}
{"id": "PMID:480453", "title": "In vitro translation of adenovirus type 12-specific mRNA isolated from infected and transformed cells.", "content": "The early and late gene products of human adenovirus type 12 (Ad12), as well as the viral proteins synthesized in an Ad12-transformed cell line, were identified by translation of viral mRNA in an in vitro protein-synthesizing system. Cytoplasmic RNA was isolated from permissive KB or nonpermissive BHK cells infected with Ad12 and from Ad12-transformed HA12/7 cells. Virus-specific RNA was selected by hybridization to Ad12 DNA covalently bound to cellulose. Viral RNA was then translated in a fractionated rabbit reticulocyte cell-free system or in wheat germ S-30 extracts. The proteins synthesized were characterized by immunoprecipitation and subsequent electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. RNA prepared from KB cells late after infection with Ad12 elicited the synthesis of most of the structural polypeptides of the virion and at least two presumably nonstructural Ad12 proteins. When viral RNA isolated early after infection of KB cells with Ad12 was translated in vitro, 10 polypeptides were observed: E-68K, E-50K, E-42K, E-39K, E-34K, E-21K, E-19K, E-13K, E-12K, and E-10K. Ad12-specific RNA was also isolated from the Ad12-transformed hamster cell line HA12/7, which contains several copies of the Ad12 genome integrated in the host genome. The RNA codes for at least seven polypeptides with molecular weights very similar to those of the early viral proteins.", "contents": "In vitro translation of adenovirus type 12-specific mRNA isolated from infected and transformed cells. The early and late gene products of human adenovirus type 12 (Ad12), as well as the viral proteins synthesized in an Ad12-transformed cell line, were identified by translation of viral mRNA in an in vitro protein-synthesizing system. Cytoplasmic RNA was isolated from permissive KB or nonpermissive BHK cells infected with Ad12 and from Ad12-transformed HA12/7 cells. Virus-specific RNA was selected by hybridization to Ad12 DNA covalently bound to cellulose. Viral RNA was then translated in a fractionated rabbit reticulocyte cell-free system or in wheat germ S-30 extracts. The proteins synthesized were characterized by immunoprecipitation and subsequent electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. RNA prepared from KB cells late after infection with Ad12 elicited the synthesis of most of the structural polypeptides of the virion and at least two presumably nonstructural Ad12 proteins. When viral RNA isolated early after infection of KB cells with Ad12 was translated in vitro, 10 polypeptides were observed: E-68K, E-50K, E-42K, E-39K, E-34K, E-21K, E-19K, E-13K, E-12K, and E-10K. Ad12-specific RNA was also isolated from the Ad12-transformed hamster cell line HA12/7, which contains several copies of the Ad12 genome integrated in the host genome. The RNA codes for at least seven polypeptides with molecular weights very similar to those of the early viral proteins."}
{"id": "PMID:480455", "title": "Adenovirus type 2 early polypeptides immunoprecipitated by antisera to five lines of adenovirus-transformed rat cells.", "content": "We have identified adenovirus type 2 (Ad2)-induced early polypeptides (EPs) and have attempted to determine which EPs are coded by each of the four early gene blocks. [35S]methionine-labeled EPs were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cycloheximide pretreatment followed by labeling in hypertonic medium (210 to 250 mM NaCl) facilitated the detection of EPs. Seven major (reproducible bands in autoradiograms) EPs were detected with molecular weights of 74,000 (74K), 21K, 19K, 15K, 13.5K, 11.5K, and 11K. Minor (weaker bands) EPs of 55K, 52K, 42K, 18K, 12K, 8.8K, and 8.3K were also often seen. To identify and map the genes for virus-coded EPs, we prepared antisera against five lines of adenovirus-transformed cells that retain different fractions of the viral genome. The lines were F17, 8617, F4, and T2C4 transformed by Ad2 virions and 5RK (clone I) transformed by transfection with the Ad5 HsuI-G fragment (map position 0 to 8). The early gene blocks retained and expressed (in part) as RNA in these cells were as follows: 5RK(I), block 1 (70% of left 8% of genome); F17, block 1; 8617, blocks 1 and 4; F4 blocks 1, 2, and 4; T2C4, blocks 1, 2, 3, and 4. The following major EPs were immunoprecipitated: 15K by all antisera; 53K and 14.5K by F17, T2C4, 8617, and F4 antisera; 11.5K by T2C4, 8617, and F4 antisera; 44K, 42K, 19K, and 13.5K by T2C4 antisera; 11K by 8617 antisera. Minor EPs of 28K, 18K, and 12K were precipitated by all antisera except 5RK(I). The 53K and 15K EPs were precipitated also from Ad2 early infected monkey cells by the F17 antiserum and by sera from hamsters bearing tumors induced by Ad1-simian virus 40. The relationships between some of the immunoprecipitated EPs were investigated by the partial proteolysis procedure. All 53K EPs are the \"same\" (i.e., highly related), all 15K EPs are the \"same,\" and all 11.5K EPs are the \"same.\" The 15K EP is highly related to the 14.5 K EP. Although less certain, all 28K EPs appeared related, as did all 18K EPs. The T2C4-specific 44K EP is probably a dimer of the 21K glycopolypeptide. The T2C4-specific 13.5K EP and the 8617-specific 11K EP appear unrelated to any other polypeptides. These immunoprecipitation data provide evidence that early gene block I (map position 1 to 11) may encode major 53K, 15K, and 14.5K polypeptides, and minor 28K, 18K, and 12K polypeptides, and that all or some of the gene for 15K and 14.5K lies within map position 1 to 8. The surprisingly complex pattern of polypeptides coded by early gene block I raises the possibility that some polypeptides may be coded by overlapping \"spliced\" mRNA's. The possible block locations of the genes for the 21K, 13.5K, and 11.5K polypeptides are discussed.", "contents": "Adenovirus type 2 early polypeptides immunoprecipitated by antisera to five lines of adenovirus-transformed rat cells. We have identified adenovirus type 2 (Ad2)-induced early polypeptides (EPs) and have attempted to determine which EPs are coded by each of the four early gene blocks. [35S]methionine-labeled EPs were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cycloheximide pretreatment followed by labeling in hypertonic medium (210 to 250 mM NaCl) facilitated the detection of EPs. Seven major (reproducible bands in autoradiograms) EPs were detected with molecular weights of 74,000 (74K), 21K, 19K, 15K, 13.5K, 11.5K, and 11K. Minor (weaker bands) EPs of 55K, 52K, 42K, 18K, 12K, 8.8K, and 8.3K were also often seen. To identify and map the genes for virus-coded EPs, we prepared antisera against five lines of adenovirus-transformed cells that retain different fractions of the viral genome. The lines were F17, 8617, F4, and T2C4 transformed by Ad2 virions and 5RK (clone I) transformed by transfection with the Ad5 HsuI-G fragment (map position 0 to 8). The early gene blocks retained and expressed (in part) as RNA in these cells were as follows: 5RK(I), block 1 (70% of left 8% of genome); F17, block 1; 8617, blocks 1 and 4; F4 blocks 1, 2, and 4; T2C4, blocks 1, 2, 3, and 4. The following major EPs were immunoprecipitated: 15K by all antisera; 53K and 14.5K by F17, T2C4, 8617, and F4 antisera; 11.5K by T2C4, 8617, and F4 antisera; 44K, 42K, 19K, and 13.5K by T2C4 antisera; 11K by 8617 antisera. Minor EPs of 28K, 18K, and 12K were precipitated by all antisera except 5RK(I). The 53K and 15K EPs were precipitated also from Ad2 early infected monkey cells by the F17 antiserum and by sera from hamsters bearing tumors induced by Ad1-simian virus 40. The relationships between some of the immunoprecipitated EPs were investigated by the partial proteolysis procedure. All 53K EPs are the \"same\" (i.e., highly related), all 15K EPs are the \"same,\" and all 11.5K EPs are the \"same.\" The 15K EP is highly related to the 14.5 K EP. Although less certain, all 28K EPs appeared related, as did all 18K EPs. The T2C4-specific 44K EP is probably a dimer of the 21K glycopolypeptide. The T2C4-specific 13.5K EP and the 8617-specific 11K EP appear unrelated to any other polypeptides. These immunoprecipitation data provide evidence that early gene block I (map position 1 to 11) may encode major 53K, 15K, and 14.5K polypeptides, and minor 28K, 18K, and 12K polypeptides, and that all or some of the gene for 15K and 14.5K lies within map position 1 to 8. The surprisingly complex pattern of polypeptides coded by early gene block I raises the possibility that some polypeptides may be coded by overlapping \"spliced\" mRNA's. The possible block locations of the genes for the 21K, 13.5K, and 11.5K polypeptides are discussed."}
{"id": "PMID:480454", "title": "Post-translational modification of Rauscher leukemia virus precursor polyproteins encoded by the gag gene.", "content": "Post-translational modifications of retrovirus gag gene-encoded polyproteins include proteolytic cleavage, phosphorylation, and glycosylation. To study the sequence of these events, we labeled JLS-V9 cells chronically infected with Rauscher murine leukemia virus in pulse-chase experiments with the radioactive precursors [35S]methionine, [14C]mannose, [3H]glucosamine, and [32P]phosphate. Newly synthesized gag polyproteins which incorporated label, and the modified products derived from them, were identified by immunoprecipitation of cell lysates with anti-p30 rabbit serum, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Pulse-chase experiments were carried out in the presence as well as in the absence of tunicamycin, an inhibitor of glycosylation. Among the three major polyproteins synthesized in the absence of tunicamycin, two were found to be glycosylated but not phosphorylated. These were designated gPr80gag and gP94gag. Both shared identical [35S]methionine peptides with Pr65gag and p30. Of the two nonglycosylated precursors, Pr65gag and Pr75gag, only Pr65gag was found to be detectably phosphorylated, and Pr75gag could be readily identified only when glycosylation was inhibited. On the basis of these results, a scheme for the post-translational modification of gag polyproteins is proposed. According to this scheme the gag gene-encoded polyproteins are processed from a common precursor, Pr75gag, by two divergent pathways: one leading through the intermediate Pr65gag to internal virion components via cleavage and phosphorylation and the other via tunicamycin-sensitive mannosylation to the intermediate gPr80gag, which is further glycosylated to yield cell surface polyprotein gP94gag.", "contents": "Post-translational modification of Rauscher leukemia virus precursor polyproteins encoded by the gag gene. Post-translational modifications of retrovirus gag gene-encoded polyproteins include proteolytic cleavage, phosphorylation, and glycosylation. To study the sequence of these events, we labeled JLS-V9 cells chronically infected with Rauscher murine leukemia virus in pulse-chase experiments with the radioactive precursors [35S]methionine, [14C]mannose, [3H]glucosamine, and [32P]phosphate. Newly synthesized gag polyproteins which incorporated label, and the modified products derived from them, were identified by immunoprecipitation of cell lysates with anti-p30 rabbit serum, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Pulse-chase experiments were carried out in the presence as well as in the absence of tunicamycin, an inhibitor of glycosylation. Among the three major polyproteins synthesized in the absence of tunicamycin, two were found to be glycosylated but not phosphorylated. These were designated gPr80gag and gP94gag. Both shared identical [35S]methionine peptides with Pr65gag and p30. Of the two nonglycosylated precursors, Pr65gag and Pr75gag, only Pr65gag was found to be detectably phosphorylated, and Pr75gag could be readily identified only when glycosylation was inhibited. On the basis of these results, a scheme for the post-translational modification of gag polyproteins is proposed. According to this scheme the gag gene-encoded polyproteins are processed from a common precursor, Pr75gag, by two divergent pathways: one leading through the intermediate Pr65gag to internal virion components via cleavage and phosphorylation and the other via tunicamycin-sensitive mannosylation to the intermediate gPr80gag, which is further glycosylated to yield cell surface polyprotein gP94gag."}
{"id": "PMID:480456", "title": "Gene 24-controlled osmotic shock resistance in bacteriophage T4: probable multiple gene functions.", "content": "By use of mixed infections with conditional lethal mutations in the head genes and an osmotic shock-resistant mutant we have demonstrated that osmotic shock resistance is controlled by gene 24. Using acrylamide gel electrophoresis combined with the \"immune replicate\" technique, we confirmed the positions of gene products 24 and 24* (P24 and P24*). In this paper we have still used the notation \"P24,\" etc., for designating the product of gene 23, etc., although we prefer and use in general the designation \"gp23\" as introduced by Casjens and King (Annu. Rev. Biochem. 44:585, 1975). The reason for using the old notation is because the illustrations were prepared several years ago.) P24 ts showed a significantly slower mobility. Both osmotic shock-resistant and -sensitive mature phages contain 24*. Giants constructed with the Osr phage showed the same surface lattice as normal phage. Through temperature-shift experiments with 24(tsL90) alone and in combinations, we studied the phages which are matured after the shift to permissive temperature in the absence of new protein synthesis. Our results strongly suggest that only a fraction of the total phage complement of gene 24-controlled proteins is involved in determining the phenotype of shock resistance, and the remainder is necessary to mature the head.", "contents": "Gene 24-controlled osmotic shock resistance in bacteriophage T4: probable multiple gene functions. By use of mixed infections with conditional lethal mutations in the head genes and an osmotic shock-resistant mutant we have demonstrated that osmotic shock resistance is controlled by gene 24. Using acrylamide gel electrophoresis combined with the \"immune replicate\" technique, we confirmed the positions of gene products 24 and 24* (P24 and P24*). In this paper we have still used the notation \"P24,\" etc., for designating the product of gene 23, etc., although we prefer and use in general the designation \"gp23\" as introduced by Casjens and King (Annu. Rev. Biochem. 44:585, 1975). The reason for using the old notation is because the illustrations were prepared several years ago.) P24 ts showed a significantly slower mobility. Both osmotic shock-resistant and -sensitive mature phages contain 24*. Giants constructed with the Osr phage showed the same surface lattice as normal phage. Through temperature-shift experiments with 24(tsL90) alone and in combinations, we studied the phages which are matured after the shift to permissive temperature in the absence of new protein synthesis. Our results strongly suggest that only a fraction of the total phage complement of gene 24-controlled proteins is involved in determining the phenotype of shock resistance, and the remainder is necessary to mature the head."}
{"id": "PMID:480457", "title": "Intermolecular duplexes formed from polyadenylylated vaccinia virus RNA.", "content": "Approximately 15% of the polyadenylic acid-containing cytoplasmic RNA labeled from 5 to 7 h after vaccinia virus infection formed intermolecular duplex structures characterized as double-stranded RNA by RNase resistance, density in Cs2SO4, base composition, chromatography on cellulose, and ability to inhibit reticulocyte cell-free protein synthesis. Both sucrose gradient sedimentation and electron microscopic analysis indicated that the double-stranded regions were several hundred to more than a thousand nucleotide base pairs long. The double-stranded RNA, after denaturation, hybridized to approximately 25% of the vaccinia virus genome, whereas total late RNA hybridized to 42%. The finding that the duplex RNA, after denaturation, hybridized to most HindIII restriction endonuclease fragments of vaccinia virus DNA indicated that symmetrical transcription is not confined to the terminal inverted repeat sequence or to one contiguous region of the genome. Although relatively little labeled, early, polyadenylic acid-containing RNA formed RNase-resistant hybrids upon self-annealing, the percentage increased upon addition of unlabeled late RNA, indicating that the latter contains \"anti-early\" sequences.", "contents": "Intermolecular duplexes formed from polyadenylylated vaccinia virus RNA. Approximately 15% of the polyadenylic acid-containing cytoplasmic RNA labeled from 5 to 7 h after vaccinia virus infection formed intermolecular duplex structures characterized as double-stranded RNA by RNase resistance, density in Cs2SO4, base composition, chromatography on cellulose, and ability to inhibit reticulocyte cell-free protein synthesis. Both sucrose gradient sedimentation and electron microscopic analysis indicated that the double-stranded regions were several hundred to more than a thousand nucleotide base pairs long. The double-stranded RNA, after denaturation, hybridized to approximately 25% of the vaccinia virus genome, whereas total late RNA hybridized to 42%. The finding that the duplex RNA, after denaturation, hybridized to most HindIII restriction endonuclease fragments of vaccinia virus DNA indicated that symmetrical transcription is not confined to the terminal inverted repeat sequence or to one contiguous region of the genome. Although relatively little labeled, early, polyadenylic acid-containing RNA formed RNase-resistant hybrids upon self-annealing, the percentage increased upon addition of unlabeled late RNA, indicating that the latter contains \"anti-early\" sequences."}
{"id": "PMID:480458", "title": "Generation of defective interfering particles of Semliki Forest virus in a clone of Aedes albopictus (mosquito) cells.", "content": "Serial undiluted passage of Semliki Forest virus in a clone of Aedes albopictus cells resulted in a marked decrease in infectious virus yields due to the generation and accumulation of defective interfering particles. Virus from the third passage had a high particle/infectivity ratio and interfered specifically with homologous but not heterologous standard virus replication. Two RNA species of molecular weights 0.78 X 10(6) and 0.61 X 10(6) were the major RNA components of purified passage 4 virus. These RNA species were also the predominant virus RNA species detected in cells infected with passage 3 virus. Synthesis of standard virus RNA and virus-specified protein was much reduced in passage 3 virus-infected cells. Interference with standard virus replication and the synthesis of large amounts of defective interfering RNA were also observed in chicken embryo cells infected with passage 3 virus from mosquito cells.", "contents": "Generation of defective interfering particles of Semliki Forest virus in a clone of Aedes albopictus (mosquito) cells. Serial undiluted passage of Semliki Forest virus in a clone of Aedes albopictus cells resulted in a marked decrease in infectious virus yields due to the generation and accumulation of defective interfering particles. Virus from the third passage had a high particle/infectivity ratio and interfered specifically with homologous but not heterologous standard virus replication. Two RNA species of molecular weights 0.78 X 10(6) and 0.61 X 10(6) were the major RNA components of purified passage 4 virus. These RNA species were also the predominant virus RNA species detected in cells infected with passage 3 virus. Synthesis of standard virus RNA and virus-specified protein was much reduced in passage 3 virus-infected cells. Interference with standard virus replication and the synthesis of large amounts of defective interfering RNA were also observed in chicken embryo cells infected with passage 3 virus from mosquito cells."}
{"id": "PMID:480459", "title": "JC human papovavirus replication in human amnion cells.", "content": "JC human papovavirus was found to replicate in primary human amnion cells. The virus has undergone eight passages in amnion cells and was identified by serological methods as JC virus. By restriction endonuclease analysis of the viral DNA, the fragments observed were identical to those previously reported for the prototype strain.", "contents": "JC human papovavirus replication in human amnion cells. JC human papovavirus was found to replicate in primary human amnion cells. The virus has undergone eight passages in amnion cells and was identified by serological methods as JC virus. By restriction endonuclease analysis of the viral DNA, the fragments observed were identical to those previously reported for the prototype strain."}
{"id": "PMID:480460", "title": "Early events in parvovirus replication: lack of integration by minute virus of mice into host cell DNA.", "content": "Viral DNA sequences were not detected in high-molecular-weight host DNA until well after the onset of viral DNA replication.", "contents": "Early events in parvovirus replication: lack of integration by minute virus of mice into host cell DNA. Viral DNA sequences were not detected in high-molecular-weight host DNA until well after the onset of viral DNA replication."}
{"id": "PMID:480461", "title": "Heterologous interference in Aedes albopictus cells infected with alphaviruses.", "content": "Maximum amounts of 42S and 26S single-stranded viral RNA and viral structural proteins were synthesized in Aedes albopictus cells at 24 h after Sindbis virus infection. Thereafter, viral RNA and protein syntheses were inhibited. By 3 days postinfection, only small quantities of 42S RNA and no detectable 26S RNA or structural proteins were synthesized in infected cells. Superinfection of A. albopictus cells 3 days after Sindbis virus infection with Sindbis, Semliki Forest, Una, or Chikungunya alphavirus did not lead to the synthesis of intracellular 26S viral RNA. In contrast, infection with snowshoe hare virus, a bunyavirus, induced the synthesis of snowshoe hare virus RNA in both A. Ablpictus cells 3 days after Sindbis virus infection and previously uninfected mosquito cells. These results suggested that at 3 days after infection with Sindbis virus, mosquito cells restricted the replication of both homologous and heterologous alphaviruses but remained susceptible to infection with a bunyavirus. In superinfection experiments the the alphaviruses were differentiated on the basis of plaque morphology and the electrophoretic mobility of their intracellular 26S viral RNA species. Thus, it was shown that within 1 h after infection with eigher Sindbis or Chikungunya virus, A. albopictus cells were resistant to superinfection with Sindbis, Chikungunya, Una, and Semliki Forest viruses. Infected cultures were resistant to superinfection with the homologous virus indefinitely, but maximum resistance to superinfection with heterologous alphaviruses lasted for approximately 8 days. After that time, infected cultures supported the replication of heterologous alphaviruses to the same extent as did persistently infected cultures established months previously. However, the titer of heterologous alphavirus produced after superinfection of persistently infected cultures was 10- to 50-fold less than that produced by an equal number of previously uninfected A. albopictus cells. Only a small proportion (8 to 10%) of the cells in a persistently infected culture was capable of supporting the replication of a heterologous alphavirus.", "contents": "Heterologous interference in Aedes albopictus cells infected with alphaviruses. Maximum amounts of 42S and 26S single-stranded viral RNA and viral structural proteins were synthesized in Aedes albopictus cells at 24 h after Sindbis virus infection. Thereafter, viral RNA and protein syntheses were inhibited. By 3 days postinfection, only small quantities of 42S RNA and no detectable 26S RNA or structural proteins were synthesized in infected cells. Superinfection of A. albopictus cells 3 days after Sindbis virus infection with Sindbis, Semliki Forest, Una, or Chikungunya alphavirus did not lead to the synthesis of intracellular 26S viral RNA. In contrast, infection with snowshoe hare virus, a bunyavirus, induced the synthesis of snowshoe hare virus RNA in both A. Ablpictus cells 3 days after Sindbis virus infection and previously uninfected mosquito cells. These results suggested that at 3 days after infection with Sindbis virus, mosquito cells restricted the replication of both homologous and heterologous alphaviruses but remained susceptible to infection with a bunyavirus. In superinfection experiments the the alphaviruses were differentiated on the basis of plaque morphology and the electrophoretic mobility of their intracellular 26S viral RNA species. Thus, it was shown that within 1 h after infection with eigher Sindbis or Chikungunya virus, A. albopictus cells were resistant to superinfection with Sindbis, Chikungunya, Una, and Semliki Forest viruses. Infected cultures were resistant to superinfection with the homologous virus indefinitely, but maximum resistance to superinfection with heterologous alphaviruses lasted for approximately 8 days. After that time, infected cultures supported the replication of heterologous alphaviruses to the same extent as did persistently infected cultures established months previously. However, the titer of heterologous alphavirus produced after superinfection of persistently infected cultures was 10- to 50-fold less than that produced by an equal number of previously uninfected A. albopictus cells. Only a small proportion (8 to 10%) of the cells in a persistently infected culture was capable of supporting the replication of a heterologous alphavirus."}
{"id": "PMID:480462", "title": "Role of virus variants and cells in maintenance of persistent infection by measles virus.", "content": "Hamster embryo fibroblasts persistently infected with a derivative of the Schwarz vaccine strain of measles virus spontaneously released virus particles with an average buoyant density considerably lower than that of the parental virus. The released virus contained all of the measles virus structural proteins and interfered with replication of standard virus. All of the virus structural proteins were associated with a membrane-free cytoplasmic extract from the persistently infected cells. Membrane-free cytoplasmic extracts prepared from Vero cells lytically infected with Schwarz strain measles contained little or no virus envelope structural protein. Maintenance of persistent infection may involve both the presence of virus variants and a defect in the ability of the infected cell to replicate the virus efficiently.", "contents": "Role of virus variants and cells in maintenance of persistent infection by measles virus. Hamster embryo fibroblasts persistently infected with a derivative of the Schwarz vaccine strain of measles virus spontaneously released virus particles with an average buoyant density considerably lower than that of the parental virus. The released virus contained all of the measles virus structural proteins and interfered with replication of standard virus. All of the virus structural proteins were associated with a membrane-free cytoplasmic extract from the persistently infected cells. Membrane-free cytoplasmic extracts prepared from Vero cells lytically infected with Schwarz strain measles contained little or no virus envelope structural protein. Maintenance of persistent infection may involve both the presence of virus variants and a defect in the ability of the infected cell to replicate the virus efficiently."}
{"id": "PMID:480463", "title": "Insensitivity of a ricin-resistant mutant of Chinese hamster ovary cells to fusion induced by Newcastle disease virus.", "content": "The role of membrane components in the interaction of cells with Newcastle disease virus (NDV) was studied using a ricin-resistant mutant of Chinese hamster ovary cells (CHO-15B), in which there is a deficiency in distal saccharides at the plasma membrane. Compared to the parental wild type, the mutant was shown to be 4- to 10-fold less sensitive to either fusion from without or fusion from within induced by NDV. The mutant and wild type were nearly indistinguishable with respect to other interactions with NDV. Viral attachment was investigated with 125I-labeled NDV and found to be comparable in both lines. Functionally equivalent amounts of hemagglutinin were produced, as measured by the fraction of cells positive for hemadsorption, or by the number of erythrocytes adsorbed per cell. No significant differences in the morphogenesis or yield of progeny virus were seen. The ability of the mutant to produce a fusion factor was measured by transfer of infected cells to uninfected monolayers. Infected CHO-15B cells were capable of inducing fusion normally in the indicator wild-type monolayers, but were incapable of inducing fusion in mutant monolayers. These results suggest that the insensitivity of the CHO-15B mutants to fusion may be due to inhibition of an early virus-cell interaction subsequent to viral attachment, whereas other events in infection appear to be unaffected by the cell surface mutation.", "contents": "Insensitivity of a ricin-resistant mutant of Chinese hamster ovary cells to fusion induced by Newcastle disease virus. The role of membrane components in the interaction of cells with Newcastle disease virus (NDV) was studied using a ricin-resistant mutant of Chinese hamster ovary cells (CHO-15B), in which there is a deficiency in distal saccharides at the plasma membrane. Compared to the parental wild type, the mutant was shown to be 4- to 10-fold less sensitive to either fusion from without or fusion from within induced by NDV. The mutant and wild type were nearly indistinguishable with respect to other interactions with NDV. Viral attachment was investigated with 125I-labeled NDV and found to be comparable in both lines. Functionally equivalent amounts of hemagglutinin were produced, as measured by the fraction of cells positive for hemadsorption, or by the number of erythrocytes adsorbed per cell. No significant differences in the morphogenesis or yield of progeny virus were seen. The ability of the mutant to produce a fusion factor was measured by transfer of infected cells to uninfected monolayers. Infected CHO-15B cells were capable of inducing fusion normally in the indicator wild-type monolayers, but were incapable of inducing fusion in mutant monolayers. These results suggest that the insensitivity of the CHO-15B mutants to fusion may be due to inhibition of an early virus-cell interaction subsequent to viral attachment, whereas other events in infection appear to be unaffected by the cell surface mutation."}
{"id": "PMID:480464", "title": "Multiple integration sites for Moloney murine leukemia virus in productively infected mouse fibroblasts.", "content": "The integration sites for viral DNA in cells infected with Moloney murine leukemia virus (M-MuLV) were studied by restriction endonuclease cleavage of cellular DNA followed by electrophoresis in agarose gels, blot transfer to nitrocellulose, and detection by M-MuLV-related sequences by hybridization with high-specific-activity 32P-labeled M-MuLV complementary DNA. When EcoRI was used to cleave cellular DNA, numerous DNA fragments with sequence homology to M-MuLV were detected in uninfected mouse cell DNA. These endogenous sequences are mouse specific since they are not detectable in rat cell DNA, and are related to the 38S genomic RNA of M-MuLV. Infected cells contain additional M-MuLV-specific DNA fragments which are not detected in uninfected cells. Different patterns of M-MuLV-specific DNA fragments were detected in each cloned infected line examined. These data suggest the existence of multiple sites for integration of M-MuLV DNA in infected mouse fibroblasts. Cleavage of infected cell DNA with BamHI, which cleaves M-MuLV viral DNA at least twice, released the internal BamHI B fragment from each infected line, confirming the presence of integrated M-MuLV DNA sequences in each infected cell line which retain some features of the sequence organization of unintegrated M-MuLV DNA.", "contents": "Multiple integration sites for Moloney murine leukemia virus in productively infected mouse fibroblasts. The integration sites for viral DNA in cells infected with Moloney murine leukemia virus (M-MuLV) were studied by restriction endonuclease cleavage of cellular DNA followed by electrophoresis in agarose gels, blot transfer to nitrocellulose, and detection by M-MuLV-related sequences by hybridization with high-specific-activity 32P-labeled M-MuLV complementary DNA. When EcoRI was used to cleave cellular DNA, numerous DNA fragments with sequence homology to M-MuLV were detected in uninfected mouse cell DNA. These endogenous sequences are mouse specific since they are not detectable in rat cell DNA, and are related to the 38S genomic RNA of M-MuLV. Infected cells contain additional M-MuLV-specific DNA fragments which are not detected in uninfected cells. Different patterns of M-MuLV-specific DNA fragments were detected in each cloned infected line examined. These data suggest the existence of multiple sites for integration of M-MuLV DNA in infected mouse fibroblasts. Cleavage of infected cell DNA with BamHI, which cleaves M-MuLV viral DNA at least twice, released the internal BamHI B fragment from each infected line, confirming the presence of integrated M-MuLV DNA sequences in each infected cell line which retain some features of the sequence organization of unintegrated M-MuLV DNA."}
{"id": "PMID:480465", "title": "Structure of the membrane protein of influenza virus. I. Isolation and characterization of cyanogen bromide cleavage products.", "content": "After cleavage of the membrane (M) protein of influenza A/WSN virus by using cyanogen bromide (CNBr), six peptide peaks representing approximate molecular weights of 6,000, 4,000, 2,200, 1,600, 1,200, and 1,000 were resolved by gel filtration on BioGel P6. Analysis by thin-layer chromatography indicates that the first, second, fourth, and fifth peaks contain single-peptide components, whereas the third and sixth peaks contain more than one peptide. By using Whatman CM52 ion-exchange chromatography in 5 M urea, four peptides were resolved from the third BioGel P6 peak. The amino acid composition of each of the purified peptides has been determined, and partial sequences were obtained for several peptides. Based on finding a blocked amino terminal residue, the 6,000-dalton fragment appears to contain the amino terminus of the M protein, whereas the carboxy terminal peptide was identified as a 2,000-dalton peptide.", "contents": "Structure of the membrane protein of influenza virus. I. Isolation and characterization of cyanogen bromide cleavage products. After cleavage of the membrane (M) protein of influenza A/WSN virus by using cyanogen bromide (CNBr), six peptide peaks representing approximate molecular weights of 6,000, 4,000, 2,200, 1,600, 1,200, and 1,000 were resolved by gel filtration on BioGel P6. Analysis by thin-layer chromatography indicates that the first, second, fourth, and fifth peaks contain single-peptide components, whereas the third and sixth peaks contain more than one peptide. By using Whatman CM52 ion-exchange chromatography in 5 M urea, four peptides were resolved from the third BioGel P6 peak. The amino acid composition of each of the purified peptides has been determined, and partial sequences were obtained for several peptides. Based on finding a blocked amino terminal residue, the 6,000-dalton fragment appears to contain the amino terminus of the M protein, whereas the carboxy terminal peptide was identified as a 2,000-dalton peptide."}
{"id": "PMID:480466", "title": "M viral RNA segment of bunyaviruses codes for two glycoproteins, G1 and G2.", "content": "Tryptic peptide digests of the two viral glycoproteins (G1 and G2) of snowshow hare (SSH) virus, La Crosse, La Crosse (LAC) virus, and an SSH/LAC recombinant virus which has a large (L)/medium (M)/small (S) RNA segment genome composition of SSH/LAC/SSH were analyzed by ion-exchange column chromatography. The analyses prove that the M RNA species of bunyaviruses codes for the two viral glycoproteins.", "contents": "M viral RNA segment of bunyaviruses codes for two glycoproteins, G1 and G2. Tryptic peptide digests of the two viral glycoproteins (G1 and G2) of snowshow hare (SSH) virus, La Crosse, La Crosse (LAC) virus, and an SSH/LAC recombinant virus which has a large (L)/medium (M)/small (S) RNA segment genome composition of SSH/LAC/SSH were analyzed by ion-exchange column chromatography. The analyses prove that the M RNA species of bunyaviruses codes for the two viral glycoproteins."}
{"id": "PMID:480467", "title": "Program of bacteriophage gh-1 DNA transcription in infected Pseudomonas putida.", "content": "The program of transcription in phage gh-1-infected Pseudomonas putida was examined. It was found that the host P. putida RNA polymerase transcribes early RNA from the L strand of gh-1 DNA during the initial stages of infection. The host RNA polymerase is also undoubtedly responsible for transcription of complementary RNA late in the infectious cycle because complementary RNA was not transcribed when rifampin was added to the infected cell culture. The gh-1-induced RNA polymerase transcribes late RNA from the L strand of gh-1 DNA late in the infectious cycle. The P. putida RNA polymerase transcribed only early RNA from primarily the L strand of gh-1 DNA in vitro when the molar ratio of enzyme to gh-1 DNA was 0.5. When the molar ratio was 50 the P. putida RNA polymerase transcribed RNA from the H strand of gh-1 DNA as well as complementary RNA. Thgh-1 RNA polymerase transcribed only the L strand of gh-1 DNA in vitro but transcribed both early and late RNA.", "contents": "Program of bacteriophage gh-1 DNA transcription in infected Pseudomonas putida. The program of transcription in phage gh-1-infected Pseudomonas putida was examined. It was found that the host P. putida RNA polymerase transcribes early RNA from the L strand of gh-1 DNA during the initial stages of infection. The host RNA polymerase is also undoubtedly responsible for transcription of complementary RNA late in the infectious cycle because complementary RNA was not transcribed when rifampin was added to the infected cell culture. The gh-1-induced RNA polymerase transcribes late RNA from the L strand of gh-1 DNA late in the infectious cycle. The P. putida RNA polymerase transcribed only early RNA from primarily the L strand of gh-1 DNA in vitro when the molar ratio of enzyme to gh-1 DNA was 0.5. When the molar ratio was 50 the P. putida RNA polymerase transcribed RNA from the H strand of gh-1 DNA as well as complementary RNA. Thgh-1 RNA polymerase transcribed only the L strand of gh-1 DNA in vitro but transcribed both early and late RNA."}
{"id": "PMID:480468", "title": "Reovirus serotypes 1 and 3 differ in their in vitro association with microtubules.", "content": "Utilizing negative-stain electron microscopy in which similar concentrations of reovirus types 1 and 3 are incubated with a carbon support film containing chick brain, rabbit brain, or HeLa cell microtubules, 81% of the type 1 and 56% of type 3 exhibited an association with the apparent \"edge\" of the microtubule. This implies that there is a high level of specific affinity for type 1 but not for type 3 to microtubules, since it has previously been determined that only 50% of randomly associated particles would be associated with the edge. The high edge binding of reovirus type 1 is virtually independent of the origin of microtubule, or of whether microtubules or virus has been initially adhered to the support film. On the other hand, reovirus type 1-specific antiserum reduced the edge binding or reovirus type 1 to 45%, whereas type 3 specific antiserum caused no less (within the variability of the assay) of the edge binding of reovirus type 1 to microtubules (76% edge bound). High edge binding of reovirus type 1 to microtubules is correlated with the presence of type 1 or sigma 1 polypeptide. This minor outer capsid polypeptide is encoded in the S1 double-stranded RNA segment and is the viral hemagglutinin and neutralization antigen. Recombinant reovirus clones containing the S1 double-stranded RNA segment of type 1 (80 and 802) show about 85% edge binding, as compared to a value of 42% for clones and the S1 gene of type 3 (204. Electron microscopy of purified reovirus types 1 and 3 by negative staining reveals that type 1 and 802 capsomers are distinctly visualized, whereas those of type 3 and 204 appear diffuse. Thus, the greater in vitro binding of type 1 to microtubules may reflect an increased accessibility of certain of its outer capsomers, and thereby, sigma 1 polypeptides to microtubules. Examination of its outer sections of reovirus type 1- and 3-infected cells at 24 to 48 h postinfection at 31 degrees C showed that about eight times as many viral factoris in type 1-infected cells exhibited an extensive association of virus particles with microtubules, as compared to viral factories of type 3-infected cells. Thus, both in vivo and in vitro there appears to be a greater specificity for the association of reovirus type 1 particles with microtubules, as compared to reovirus type 3 particles.", "contents": "Reovirus serotypes 1 and 3 differ in their in vitro association with microtubules. Utilizing negative-stain electron microscopy in which similar concentrations of reovirus types 1 and 3 are incubated with a carbon support film containing chick brain, rabbit brain, or HeLa cell microtubules, 81% of the type 1 and 56% of type 3 exhibited an association with the apparent \"edge\" of the microtubule. This implies that there is a high level of specific affinity for type 1 but not for type 3 to microtubules, since it has previously been determined that only 50% of randomly associated particles would be associated with the edge. The high edge binding of reovirus type 1 is virtually independent of the origin of microtubule, or of whether microtubules or virus has been initially adhered to the support film. On the other hand, reovirus type 1-specific antiserum reduced the edge binding or reovirus type 1 to 45%, whereas type 3 specific antiserum caused no less (within the variability of the assay) of the edge binding of reovirus type 1 to microtubules (76% edge bound). High edge binding of reovirus type 1 to microtubules is correlated with the presence of type 1 or sigma 1 polypeptide. This minor outer capsid polypeptide is encoded in the S1 double-stranded RNA segment and is the viral hemagglutinin and neutralization antigen. Recombinant reovirus clones containing the S1 double-stranded RNA segment of type 1 (80 and 802) show about 85% edge binding, as compared to a value of 42% for clones and the S1 gene of type 3 (204. Electron microscopy of purified reovirus types 1 and 3 by negative staining reveals that type 1 and 802 capsomers are distinctly visualized, whereas those of type 3 and 204 appear diffuse. Thus, the greater in vitro binding of type 1 to microtubules may reflect an increased accessibility of certain of its outer capsomers, and thereby, sigma 1 polypeptides to microtubules. Examination of its outer sections of reovirus type 1- and 3-infected cells at 24 to 48 h postinfection at 31 degrees C showed that about eight times as many viral factoris in type 1-infected cells exhibited an extensive association of virus particles with microtubules, as compared to viral factories of type 3-infected cells. Thus, both in vivo and in vitro there appears to be a greater specificity for the association of reovirus type 1 particles with microtubules, as compared to reovirus type 3 particles."}
{"id": "PMID:480469", "title": "In vivo assembly of a biological membrane of defined size, shape, and lipid composition.", "content": "At restrictive temperature, mutant ts1 of bacteriophage PM2 makes membrane vesicles inside infected Alteromonas espejiana. A shift from restrictive to permissive temperature resulted in rapid maturation to infectious virions. The membrane vesicles were isolated from cellular membranes by sucrose density gradient centrifugation. Analysis of the unique peak at rho = 1.190 g/cm3 showed spheres of two diameters, 50 nm and 54 nm. The wild-type virus is icosahedral with an average diameter of 60 nm. Gel electrophoresis indicated the absence in the vesicles of the coat and spike proteins. sp27 and sp43, respectively, and the presence of only one viral structural protein, sp6.6. DNA was also present. The lipid in the vesicles was composed of phosphatidylglycerol and phosphatidylethanolamine in a proportion similar to that of the wild-type virus, whose ratio is nearly the inverse of that found in the host membrane. Thus, membrane vesicles made by mutant ts1 resembled the membrane of the wild-type virus in size, shape, and lipid composition, but contained only one of the four structural proteins of the virus. This hydrophobic protein, sp6.6 may be responsible for stimulating membrane morphogenesis.", "contents": "In vivo assembly of a biological membrane of defined size, shape, and lipid composition. At restrictive temperature, mutant ts1 of bacteriophage PM2 makes membrane vesicles inside infected Alteromonas espejiana. A shift from restrictive to permissive temperature resulted in rapid maturation to infectious virions. The membrane vesicles were isolated from cellular membranes by sucrose density gradient centrifugation. Analysis of the unique peak at rho = 1.190 g/cm3 showed spheres of two diameters, 50 nm and 54 nm. The wild-type virus is icosahedral with an average diameter of 60 nm. Gel electrophoresis indicated the absence in the vesicles of the coat and spike proteins. sp27 and sp43, respectively, and the presence of only one viral structural protein, sp6.6. DNA was also present. The lipid in the vesicles was composed of phosphatidylglycerol and phosphatidylethanolamine in a proportion similar to that of the wild-type virus, whose ratio is nearly the inverse of that found in the host membrane. Thus, membrane vesicles made by mutant ts1 resembled the membrane of the wild-type virus in size, shape, and lipid composition, but contained only one of the four structural proteins of the virus. This hydrophobic protein, sp6.6 may be responsible for stimulating membrane morphogenesis."}
{"id": "PMID:480471", "title": "Mutagenic specificity in reovirus.", "content": "Mutagenic specificity in response to chemical mutagens has been observed with certain temperature-sensitive mutants of reovirus type 3. One mutant induced by nitrous acid reverted specifically with nitrous acid. Three nitrosoguanidine-induced mutants reverted after nitrous acid treatment. These findings thus suggest that analysis of chemical induction of reversion from the temperature-sensitive phenotype may be a useful approach for studying the nature of mutation in animal viruses.", "contents": "Mutagenic specificity in reovirus. Mutagenic specificity in response to chemical mutagens has been observed with certain temperature-sensitive mutants of reovirus type 3. One mutant induced by nitrous acid reverted specifically with nitrous acid. Three nitrosoguanidine-induced mutants reverted after nitrous acid treatment. These findings thus suggest that analysis of chemical induction of reversion from the temperature-sensitive phenotype may be a useful approach for studying the nature of mutation in animal viruses."}
{"id": "PMID:480470", "title": "Comparison of late mRNA splicing among class B and class C adenoviruses.", "content": "Adenovirus class B (Ad3 and Ad7) and class C (Ad1, Ad5, and Ad6) late r-strand mRNA's were found to have segmented 5' leaders. These leaders were very similar among serotype within a class but differed in sequence from the leaders on late mRNA's of a different class. However, the leader components of class B viruses mapped at essentially the same map coordinates as those of class C viruses. The 5' coordinates of the main bodies of class B messages to which the tripartite leaders are attached as well as the map positions of several of their early mRNA's were very similar to those of Ad2 transcripts. Infrequent examples of late r-strand polysomal RNAs of Ad3 and Ad7 had, in addition to the three common leader segments, a fourth leader segment derived from RNA encoded at various sites between the second and third leaders. The extra components formed several distinct groups. These molecules are presumably intermediates in the splicing processes that generate mature messages.", "contents": "Comparison of late mRNA splicing among class B and class C adenoviruses. Adenovirus class B (Ad3 and Ad7) and class C (Ad1, Ad5, and Ad6) late r-strand mRNA's were found to have segmented 5' leaders. These leaders were very similar among serotype within a class but differed in sequence from the leaders on late mRNA's of a different class. However, the leader components of class B viruses mapped at essentially the same map coordinates as those of class C viruses. The 5' coordinates of the main bodies of class B messages to which the tripartite leaders are attached as well as the map positions of several of their early mRNA's were very similar to those of Ad2 transcripts. Infrequent examples of late r-strand polysomal RNAs of Ad3 and Ad7 had, in addition to the three common leader segments, a fourth leader segment derived from RNA encoded at various sites between the second and third leaders. The extra components formed several distinct groups. These molecules are presumably intermediates in the splicing processes that generate mature messages."}
{"id": "PMID:480472", "title": "Transcription of the bovine parvovirus genome in isolated nuclei.", "content": "Transcription of the genome of the nondefective parvovirus BPV was examined in nuclei isolated from synchronized bovine fetal spleen cells. The relative levels of total RNA polymerase and RNA polymerase I, II, and III activities in nuclei isolated from BPV-infected and mock-infected cells were found to be similar throughout the course of infection. Hybridization of RNA synthesized in isolated nuceli indicated that BPV-specific RNA synthesis began during the period of 8 to 12 h postinfection and proceeded linearly until at least 20 h postinfection. By 20 h postinfection, 5% of the total RNA synthesized in nuclei from infected cells was virus specific. BPV-specific RNA synthesis was inhibited by 95% in the presence of 0.1 microgram of alpha-amanitin per ml, suggesting that the viral genome is transcribed by cellular RNA polymerase II.", "contents": "Transcription of the bovine parvovirus genome in isolated nuclei. Transcription of the genome of the nondefective parvovirus BPV was examined in nuclei isolated from synchronized bovine fetal spleen cells. The relative levels of total RNA polymerase and RNA polymerase I, II, and III activities in nuclei isolated from BPV-infected and mock-infected cells were found to be similar throughout the course of infection. Hybridization of RNA synthesized in isolated nuceli indicated that BPV-specific RNA synthesis began during the period of 8 to 12 h postinfection and proceeded linearly until at least 20 h postinfection. By 20 h postinfection, 5% of the total RNA synthesized in nuclei from infected cells was virus specific. BPV-specific RNA synthesis was inhibited by 95% in the presence of 0.1 microgram of alpha-amanitin per ml, suggesting that the viral genome is transcribed by cellular RNA polymerase II."}
{"id": "PMID:480473", "title": "Physical map of bacteriophage BF23 DNA: restriction enzyme analysis.", "content": "Cleavage maps of bacteriophage BF23 DNA have been constructed for the restriction endonucleases SalI (3 fragments), BamHI (5 fragments), EcoRI, (8 fragments), BalI (13 fragments), and HpaI (49 fragments, 32 of which have been ordered). The maps were determined by (i) analysis of deletion mutants, (ii) digestion with two endonucleases, (iii) digestion of isolated fragments with a second enzyme, (iv) analysis of partial digests, and (v) digestion after treatment with lambda exonuclease.", "contents": "Physical map of bacteriophage BF23 DNA: restriction enzyme analysis. Cleavage maps of bacteriophage BF23 DNA have been constructed for the restriction endonucleases SalI (3 fragments), BamHI (5 fragments), EcoRI, (8 fragments), BalI (13 fragments), and HpaI (49 fragments, 32 of which have been ordered). The maps were determined by (i) analysis of deletion mutants, (ii) digestion with two endonucleases, (iii) digestion of isolated fragments with a second enzyme, (iv) analysis of partial digests, and (v) digestion after treatment with lambda exonuclease."}
{"id": "PMID:480474", "title": "Polarized injection of the bacteriophage T5 chromosome.", "content": "Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.", "contents": "Polarized injection of the bacteriophage T5 chromosome. Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first."}
{"id": "PMID:480475", "title": "Functional relationship between bacteriophages G4 and phi X174.", "content": "Mutants of bacteriophage G4 were isolated and characterized, and their mutations were mapped. They constitute six different genes, namely, A, B, E, F, G, and H. The functional relationship with bacteriophage phi X174 was determined by complementation experiments using amber mutants of phi X and amber mutants of G4. Bacteriophage phi X was able to use the products of G4 genes E, F, G, and H. In bacteriophage G4, however, only the phi X gene H product was functional.", "contents": "Functional relationship between bacteriophages G4 and phi X174. Mutants of bacteriophage G4 were isolated and characterized, and their mutations were mapped. They constitute six different genes, namely, A, B, E, F, G, and H. The functional relationship with bacteriophage phi X174 was determined by complementation experiments using amber mutants of phi X and amber mutants of G4. Bacteriophage phi X was able to use the products of G4 genes E, F, G, and H. In bacteriophage G4, however, only the phi X gene H product was functional."}
{"id": "PMID:480476", "title": "RNA-directed DNA synthesis in Moloney murine leukemia virus: interaction between the primer tRNA and the genome RNA.", "content": "Initiation of RNA-directed DNA synthesis in virions of Moloney murine leukemia virus requires a cellular tRNAPro as primer. The site(s) on the Moloney murine leukemia virus genome RNA at which functional primer molecules are bound and at which purified tRNAPro hybridizes has been located near (within 20%) the 5' end of the genome. A relatively stable duplex (temperature at which 50% dissociation has occurred, 76 degrees C) is formed between the amino acid acceptor stem of the tRNAPro and a complementary sequence in the Moloney murine leukemia virus 35S RNA. The interaction involves 19 base pairs, extending from the penultimate nucleotide at the 3' end of the tRNAPro but apparently not including the 3'-terminal adenosine residue. In most respects, the interaction between primer and template in Moloney murine leukemia virus parallels the situation in the avian leukosis-sarcoma viruses.", "contents": "RNA-directed DNA synthesis in Moloney murine leukemia virus: interaction between the primer tRNA and the genome RNA. Initiation of RNA-directed DNA synthesis in virions of Moloney murine leukemia virus requires a cellular tRNAPro as primer. The site(s) on the Moloney murine leukemia virus genome RNA at which functional primer molecules are bound and at which purified tRNAPro hybridizes has been located near (within 20%) the 5' end of the genome. A relatively stable duplex (temperature at which 50% dissociation has occurred, 76 degrees C) is formed between the amino acid acceptor stem of the tRNAPro and a complementary sequence in the Moloney murine leukemia virus 35S RNA. The interaction involves 19 base pairs, extending from the penultimate nucleotide at the 3' end of the tRNAPro but apparently not including the 3'-terminal adenosine residue. In most respects, the interaction between primer and template in Moloney murine leukemia virus parallels the situation in the avian leukosis-sarcoma viruses."}
{"id": "PMID:480477", "title": "In vivo transcription and protein synthesis capabilities of bunyaviruses: wild-type snowshoe hare virus and its temperature-sensitive group I, group II, and group I/II mutants.", "content": "The in vivo primary and secondary transcription capabilities of wild-type snowshoe hare (SSH) virus and certain of its temperature-sensitive (ts) mutants have been analyzed. The results obtained agree with in vitro studies (Bouloy et al., C.R. Acad. Sci. Paris 280:213-215, 1975; M. Bouloy and C. Hannoun, Virology 69:258-264, 1976; M. Ranki and R. Pettersson, J. Virol. 16:1420-1425, 1975) which have shown that bunyaviruses are negative-stranded RNA viruses with a virion RNA-directed RNA polymerase. The in vivo transcription studies have demonstrated that in the presence of protein synthesis inhibitors (puromycin or cycloheximide) SSH virus can synthesize viral complementary RNA (primary transcription) throughout the infection cycle. The increased levels of viral complementary RNA obtained in the absence of protein synthesis inhibitors (secondary transcription) were not markedly reduced if cells were pretreated with actinomycin D (5 mug/ml), alpha-amanitin (25 mug/ml), or rifampin (100 mug/ml), although progeny virus yields were reduced by up to 80% in the actinomycin D- and rifampin-treated cells. The in vivo transcription capabilities of SSH group I ts mutants at temperatures which were nonpermissive (40 degrees C) for virus replication gave values comparable to those obtained at permissive temperatures (33 degrees C). The SSH group I mutants appear, therefore, to be RNA-positive mutant types. When compared with their transcription capabilities at 33 degrees C, the in vivo transcription abilities of four SSH group II ts mutants (and one double group I/II ts mutant) were found to be more impaired at 40 degrees C than those of the SSH group I ts mutants or wild-type SSH virus at 40 degrees C, although the viral complementary RNA synthetic capabilities of these group II (and group I/II) mutants at 40 degrees C were significantly higher than their primary transcription capabilities (as measured at 33 degrees C in the presence of puromycin or cycloheximide). It was concluded, therefore, that these SSH group II (and double group I/II) ts mutants have an intermediate RNA phenotype. Hybridization studies using (32)P-labeled individual L, M, and S viral RNA species of SSH virus have demonstrated the presence of viral complementary RNA to all three species in extracts of cells infected with SSH ts II-30 and incubated at 33 degrees C (primary and secondary transcription) or 40 degrees C, a nonpermissive temperature for its replication. The results of pulse-labeled in vivo protein analyses indicated that greater quantities of intracellular N protein (coded for by S RNA [J. R. Gentsch and D. H. L. Bishop, J. Virol. 28:417-419, 1978]) than G1 and G2 polypeptides (coded for by M RNA [J. R. Gentsch and D. H. L. Bishop, J. Virol. 30:767-776, 1979]) were present in extracts of cells infected with wild-type SSH virus. In extracts of SSH group I, II, or I/II ts mutant-infected cells incubated at 33 degrees C, N and G1, and for the group II mutant-infected cells, G2, viral polypeptides were detected, whereas in extracts obtained from group I or II mutant virus-infected cells incubated at 40 degrees C, low levels of N and G1 polypeptides were evident.", "contents": "In vivo transcription and protein synthesis capabilities of bunyaviruses: wild-type snowshoe hare virus and its temperature-sensitive group I, group II, and group I/II mutants. The in vivo primary and secondary transcription capabilities of wild-type snowshoe hare (SSH) virus and certain of its temperature-sensitive (ts) mutants have been analyzed. The results obtained agree with in vitro studies (Bouloy et al., C.R. Acad. Sci. Paris 280:213-215, 1975; M. Bouloy and C. Hannoun, Virology 69:258-264, 1976; M. Ranki and R. Pettersson, J. Virol. 16:1420-1425, 1975) which have shown that bunyaviruses are negative-stranded RNA viruses with a virion RNA-directed RNA polymerase. The in vivo transcription studies have demonstrated that in the presence of protein synthesis inhibitors (puromycin or cycloheximide) SSH virus can synthesize viral complementary RNA (primary transcription) throughout the infection cycle. The increased levels of viral complementary RNA obtained in the absence of protein synthesis inhibitors (secondary transcription) were not markedly reduced if cells were pretreated with actinomycin D (5 mug/ml), alpha-amanitin (25 mug/ml), or rifampin (100 mug/ml), although progeny virus yields were reduced by up to 80% in the actinomycin D- and rifampin-treated cells. The in vivo transcription capabilities of SSH group I ts mutants at temperatures which were nonpermissive (40 degrees C) for virus replication gave values comparable to those obtained at permissive temperatures (33 degrees C). The SSH group I mutants appear, therefore, to be RNA-positive mutant types. When compared with their transcription capabilities at 33 degrees C, the in vivo transcription abilities of four SSH group II ts mutants (and one double group I/II ts mutant) were found to be more impaired at 40 degrees C than those of the SSH group I ts mutants or wild-type SSH virus at 40 degrees C, although the viral complementary RNA synthetic capabilities of these group II (and group I/II) mutants at 40 degrees C were significantly higher than their primary transcription capabilities (as measured at 33 degrees C in the presence of puromycin or cycloheximide). It was concluded, therefore, that these SSH group II (and double group I/II) ts mutants have an intermediate RNA phenotype. Hybridization studies using (32)P-labeled individual L, M, and S viral RNA species of SSH virus have demonstrated the presence of viral complementary RNA to all three species in extracts of cells infected with SSH ts II-30 and incubated at 33 degrees C (primary and secondary transcription) or 40 degrees C, a nonpermissive temperature for its replication. The results of pulse-labeled in vivo protein analyses indicated that greater quantities of intracellular N protein (coded for by S RNA [J. R. Gentsch and D. H. L. Bishop, J. Virol. 28:417-419, 1978]) than G1 and G2 polypeptides (coded for by M RNA [J. R. Gentsch and D. H. L. Bishop, J. Virol. 30:767-776, 1979]) were present in extracts of cells infected with wild-type SSH virus. In extracts of SSH group I, II, or I/II ts mutant-infected cells incubated at 33 degrees C, N and G1, and for the group II mutant-infected cells, G2, viral polypeptides were detected, whereas in extracts obtained from group I or II mutant virus-infected cells incubated at 40 degrees C, low levels of N and G1 polypeptides were evident."}
{"id": "PMID:480478", "title": "Tubular subviral structure produced in adenovirus-infected KB cells.", "content": "At 40 to 90 h after infection with high multiplicities of adenovirus type 2, 4 to 15% of KB cells produced relatively few intranuclear virions detectable by electron microscopy. The nuclei of these cells were found to contain long tubular structures which were made up at least in part by adenovirus structural proteins. The ends of these tubular structures were frequently terminated by morphologically normal adenovirions in varying degrees of completeness. Circumstantial evidence suggests that the production of these aberrant virus structures results from the malfunction or absence of some essential host-provided function and not from a defect in the infecting virus.", "contents": "Tubular subviral structure produced in adenovirus-infected KB cells. At 40 to 90 h after infection with high multiplicities of adenovirus type 2, 4 to 15% of KB cells produced relatively few intranuclear virions detectable by electron microscopy. The nuclei of these cells were found to contain long tubular structures which were made up at least in part by adenovirus structural proteins. The ends of these tubular structures were frequently terminated by morphologically normal adenovirions in varying degrees of completeness. Circumstantial evidence suggests that the production of these aberrant virus structures results from the malfunction or absence of some essential host-provided function and not from a defect in the infecting virus."}
{"id": "PMID:480479", "title": "Results of surgical treatment of renovascular hypertension.", "content": "We have used a comprehensive protocol to identify secondary forms of hypertension and operated upon 77 patients with functionally significant renovascular disease. The population included 52 patients with atherosclerotic renal arterial lesions (31 unilateral and 21 bilateral) and 25 patients with fibrodysplasia (19 unilateral and 6 bilateral). In the entire population there was a 90% cured-improved rate, a 5% failure rate and a 5% postoperative mortality. The cure rate was highest in the unilateral fibrodysplasia group and lowest in those with bilateral atherosclerotic disease. The choice of initial operative approach was based on an attempt to preserve renal mass; in 11 patients vascular reconstruction was attempted and these required secondary nephrectomy because of early or late failure. All 11 patients had a good result after nephrectomy. Our observations indicate that a vigorous operative approach to renovascular hypertension is beneficial once accurate demonstration of a functionally significant lesion is made.", "contents": "Results of surgical treatment of renovascular hypertension. We have used a comprehensive protocol to identify secondary forms of hypertension and operated upon 77 patients with functionally significant renovascular disease. The population included 52 patients with atherosclerotic renal arterial lesions (31 unilateral and 21 bilateral) and 25 patients with fibrodysplasia (19 unilateral and 6 bilateral). In the entire population there was a 90% cured-improved rate, a 5% failure rate and a 5% postoperative mortality. The cure rate was highest in the unilateral fibrodysplasia group and lowest in those with bilateral atherosclerotic disease. The choice of initial operative approach was based on an attempt to preserve renal mass; in 11 patients vascular reconstruction was attempted and these required secondary nephrectomy because of early or late failure. All 11 patients had a good result after nephrectomy. Our observations indicate that a vigorous operative approach to renovascular hypertension is beneficial once accurate demonstration of a functionally significant lesion is made."}
{"id": "PMID:480480", "title": "A new catheter system for permanent percutaneous nephrostomy.", "content": "A new catheter system is described for permanent percutaneous nephrostomy. The system allows the rapid, single stage placement of a large diameter drainage catheter. The technique avoids several problems associated with previous methods of percutaneous nephrostomy.", "contents": "A new catheter system for permanent percutaneous nephrostomy. A new catheter system is described for permanent percutaneous nephrostomy. The system allows the rapid, single stage placement of a large diameter drainage catheter. The technique avoids several problems associated with previous methods of percutaneous nephrostomy."}
{"id": "PMID:480481", "title": "The role of intraoperative angiography in salvaging bovine grafts for hemodialysis.", "content": "Bovine grafts used in hemodialysis frequently need declotting. Success of the declotting procedure usually is dependent upon relieving outflow obstruction. Intraoperative angiography was done during 22 declotting procedures and outflow obstructions were detected in 14 grafts. Of these 5 grafts had clinically unsuspected clots, residual to the initial declotting attempt. Four grafts had stenotic areas at the site of the venous anastomosis, which were corrected subsequently by patch grafts. Two grafts had stenotic areas in the graft owing to an infolding of the intima covered with an organized blood clot. In 2 grafts the veins draining the grafts were stenotic for a considerable length and needed revision. In 1 graft there was stenotic change at the site of the arterial anastomosis, which required revision. Of the 14 grafts 11 have remained functioning for an additional 6 to 18 months after relief of obstruction.", "contents": "The role of intraoperative angiography in salvaging bovine grafts for hemodialysis. Bovine grafts used in hemodialysis frequently need declotting. Success of the declotting procedure usually is dependent upon relieving outflow obstruction. Intraoperative angiography was done during 22 declotting procedures and outflow obstructions were detected in 14 grafts. Of these 5 grafts had clinically unsuspected clots, residual to the initial declotting attempt. Four grafts had stenotic areas at the site of the venous anastomosis, which were corrected subsequently by patch grafts. Two grafts had stenotic areas in the graft owing to an infolding of the intima covered with an organized blood clot. In 2 grafts the veins draining the grafts were stenotic for a considerable length and needed revision. In 1 graft there was stenotic change at the site of the arterial anastomosis, which required revision. Of the 14 grafts 11 have remained functioning for an additional 6 to 18 months after relief of obstruction."}
{"id": "PMID:480482", "title": "Determination of filtration fraction using the gamma scintillation camera.", "content": "A simple technique for determining effective renal plasma flow, filtration fraction and filtration rate is described. The determination depends upon the use of 131iodine labeled hippuran and 99mtechnetium labeled iron ascorbate or Sn diethylenetriaminepentaacetic acid. The filtration fraction is determined by dividing the corrected uptake for the technetium compound by the corrected uptake for radiohippuran. The uptake is simply determined by the 1 to 2-minute count over the kidneys with background subtraction and taking into account the amount injected. The technique is simple, quick and appears to be a useful clinical tool.", "contents": "Determination of filtration fraction using the gamma scintillation camera. A simple technique for determining effective renal plasma flow, filtration fraction and filtration rate is described. The determination depends upon the use of 131iodine labeled hippuran and 99mtechnetium labeled iron ascorbate or Sn diethylenetriaminepentaacetic acid. The filtration fraction is determined by dividing the corrected uptake for the technetium compound by the corrected uptake for radiohippuran. The uptake is simply determined by the 1 to 2-minute count over the kidneys with background subtraction and taking into account the amount injected. The technique is simple, quick and appears to be a useful clinical tool."}
{"id": "PMID:480483", "title": "Diuretic radionuclide urography: a non-invasive method for evaluating nephroureteral dilatation.", "content": "The diuretic radionuclide urogram is a simple, rapid and non-invasive technique to determine whether true obstruction exists in dilated nephroureteral systems. The methodology, results and interpretation of 102 studies in 41 patients are presented and compared to the conventional methods of assessing hydroureteronephrosis.", "contents": "Diuretic radionuclide urography: a non-invasive method for evaluating nephroureteral dilatation. The diuretic radionuclide urogram is a simple, rapid and non-invasive technique to determine whether true obstruction exists in dilated nephroureteral systems. The methodology, results and interpretation of 102 studies in 41 patients are presented and compared to the conventional methods of assessing hydroureteronephrosis."}
{"id": "PMID:480484", "title": "Familial factors in bladder carcinoma.", "content": "Surprisingly, little is known about host factors in cases of bladder carcinoma. We investigated 2 families prone to transitional cell carcinoma of the bladder. A high degree of pathology verification of cancer of all anatomic sites and a meticulous recording of genealogy, associated diseases and environmental exposures, when known, have allowed a more cogent appraisal of cancer etiology. It is reasonable to assume that members of the subject families may be more susceptible to variable carcinogenic exposures, a concept that is in accord with a genetic-environmental interaction hypothesis for cancer etiology. In addition to increased surveillance of high risk patients for earlier detection of bladder cancer, cancer control measures also should take into consideration preventive programs directed toward the avoidance of known carcinogenic exposures, such as cigarette smoking in high risk relatives of cancer-affected probands. We propose that the etiology of familial bladder cancer may be complex, involving possible other associated malignant neoplasms and/or certain non-neoplastic disorders, in addition to specific carcinogenic exposures. There is a serious need for the detailed reporting of families prone to bladder cancer wherein all of these potentially important associated factors are considered, so that a fuller appraisal of etiology might be achieved.", "contents": "Familial factors in bladder carcinoma. Surprisingly, little is known about host factors in cases of bladder carcinoma. We investigated 2 families prone to transitional cell carcinoma of the bladder. A high degree of pathology verification of cancer of all anatomic sites and a meticulous recording of genealogy, associated diseases and environmental exposures, when known, have allowed a more cogent appraisal of cancer etiology. It is reasonable to assume that members of the subject families may be more susceptible to variable carcinogenic exposures, a concept that is in accord with a genetic-environmental interaction hypothesis for cancer etiology. In addition to increased surveillance of high risk patients for earlier detection of bladder cancer, cancer control measures also should take into consideration preventive programs directed toward the avoidance of known carcinogenic exposures, such as cigarette smoking in high risk relatives of cancer-affected probands. We propose that the etiology of familial bladder cancer may be complex, involving possible other associated malignant neoplasms and/or certain non-neoplastic disorders, in addition to specific carcinogenic exposures. There is a serious need for the detailed reporting of families prone to bladder cancer wherein all of these potentially important associated factors are considered, so that a fuller appraisal of etiology might be achieved."}
{"id": "PMID:480485", "title": "The prognostic value of cell surface antigens in low grade, non-invasive, transitional cell carcinoma of the bladder.", "content": "Tumors in 23 patients who presented with a low grade, non-invasive transitional cell carcinoma of the bladder were studied for blood group antigens A, B or O on the cell surface. Of 14 patients without cell surface antigens initially 13 suffered an invasive tumor subsequently and 1 had diffuse carcinoma in situ. Of 9 patients with cell surface antigens initially 8 did not have an invasive recurrence during a 5 or more-year followup and 1 did. The presence or absence of blood group cell surface antigen on a low grade, non-invasive transitional cell carcinoma of the bladder would seem to have value in predicting future recurrence with muscle invasion.", "contents": "The prognostic value of cell surface antigens in low grade, non-invasive, transitional cell carcinoma of the bladder. Tumors in 23 patients who presented with a low grade, non-invasive transitional cell carcinoma of the bladder were studied for blood group antigens A, B or O on the cell surface. Of 14 patients without cell surface antigens initially 13 suffered an invasive tumor subsequently and 1 had diffuse carcinoma in situ. Of 9 patients with cell surface antigens initially 8 did not have an invasive recurrence during a 5 or more-year followup and 1 did. The presence or absence of blood group cell surface antigen on a low grade, non-invasive transitional cell carcinoma of the bladder would seem to have value in predicting future recurrence with muscle invasion."}
{"id": "PMID:480486", "title": "HLA-A and HLA-B antigens in transitional cell carcinoma of the bladder.", "content": "A possible association between HLA-A and HLA-B loci alleles and bladder carcinoma has been investigated in 44 patients with biopsy proved transitional cell carcinoma of the bladder. The patients were compared to 400 healthy controls and matched for ethnic origin. Typing was done by the standard National Institutes of Health microlymphocytotoxicity technique. Comparison between the HLA-A and HLA-B series of the patients and the controls, and the different stages of disease did not yield any significant difference.", "contents": "HLA-A and HLA-B antigens in transitional cell carcinoma of the bladder. A possible association between HLA-A and HLA-B loci alleles and bladder carcinoma has been investigated in 44 patients with biopsy proved transitional cell carcinoma of the bladder. The patients were compared to 400 healthy controls and matched for ethnic origin. Typing was done by the standard National Institutes of Health microlymphocytotoxicity technique. Comparison between the HLA-A and HLA-B series of the patients and the controls, and the different stages of disease did not yield any significant difference."}
{"id": "PMID:480487", "title": "Immunological evaluation of a streptococcal preparation (OK-432) in treatment of bladder carcinoma.", "content": "When 21 patients with bladder cancer (transitional cell carcinoma) were given local and systemic injections of a streptococcal preparation, OK-432, tumor regression was observed in 3 cases (14.3%). The marked infiltration of lymphocytes observed on histological examination of regressed tumors suggested that a host-mediated action was involved in the antitumor effect of OK-432.", "contents": "Immunological evaluation of a streptococcal preparation (OK-432) in treatment of bladder carcinoma. When 21 patients with bladder cancer (transitional cell carcinoma) were given local and systemic injections of a streptococcal preparation, OK-432, tumor regression was observed in 3 cases (14.3%). The marked infiltration of lymphocytes observed on histological examination of regressed tumors suggested that a host-mediated action was involved in the antitumor effect of OK-432."}
{"id": "PMID:480488", "title": "Bladder carcinoma treated by partial cystectomy: a review of 54 cases.", "content": "A retrospective study of 54 partial cystectomies done between 1958 and 1973 was undertaken to evaluate the proper role of the procedure in the treatment of bladder carcinoma. The operative mortality rate was 1.8% and the postoperative morbidity was low in the series. The 5-year survival rate for stages B1 (21 cases) and B2 (16 cases) lesions was 57%, with an over-all 5-year survival rate for all stages of 48%. Indications for the operation are presented and factors influencing survival are discussed.", "contents": "Bladder carcinoma treated by partial cystectomy: a review of 54 cases. A retrospective study of 54 partial cystectomies done between 1958 and 1973 was undertaken to evaluate the proper role of the procedure in the treatment of bladder carcinoma. The operative mortality rate was 1.8% and the postoperative morbidity was low in the series. The 5-year survival rate for stages B1 (21 cases) and B2 (16 cases) lesions was 57%, with an over-all 5-year survival rate for all stages of 48%. Indications for the operation are presented and factors influencing survival are discussed."}
{"id": "PMID:480489", "title": "Carcinoma of the bladder treated by segmental resection.", "content": "We herein review 45 patients with carcinoma of the bladder treated by segmental resection from 1955 to 1976. Survival rates were similar to those reported in other series and compared favorably to those found with total cystectomy. Patients with high grade (III and IV) stage A tumors experienced a 100% recurrence rate and only 40% survived more than 5 years, whereas patients with low grade (I and II) stage A tumors had a 28% recurrence rate and 86% lived 5 years or more. Therefore, it is postulated that patients with high grade lesions, even though stage A, undergo more aggressive therapy. It also was found that extensive segmental resection, even though necessitating ureteroneocystostomy, resulted in a favorable 5-year survival rate.", "contents": "Carcinoma of the bladder treated by segmental resection. We herein review 45 patients with carcinoma of the bladder treated by segmental resection from 1955 to 1976. Survival rates were similar to those reported in other series and compared favorably to those found with total cystectomy. Patients with high grade (III and IV) stage A tumors experienced a 100% recurrence rate and only 40% survived more than 5 years, whereas patients with low grade (I and II) stage A tumors had a 28% recurrence rate and 86% lived 5 years or more. Therefore, it is postulated that patients with high grade lesions, even though stage A, undergo more aggressive therapy. It also was found that extensive segmental resection, even though necessitating ureteroneocystostomy, resulted in a favorable 5-year survival rate."}
{"id": "PMID:480491", "title": "Further experience with the inflatable penile prosthesis.", "content": "Since 1974, 93 men with erectile impotence have been treated with surgical implantation of an inflatable penile prosthesis. Followup has been 6 months to 4 years. Long-term functional success was achieved in 87 patients (93%). Complications requiring 31 secondary operations occurred in 27 patients (29%).", "contents": "Further experience with the inflatable penile prosthesis. Since 1974, 93 men with erectile impotence have been treated with surgical implantation of an inflatable penile prosthesis. Followup has been 6 months to 4 years. Long-term functional success was achieved in 87 patients (93%). Complications requiring 31 secondary operations occurred in 27 patients (29%)."}
{"id": "PMID:480492", "title": "Post-lymphangiography fine needle aspiration lymph node biopsy in staging carcinoma of the prostate: preliminary report.", "content": "Five cases of clinical stage B carcinoma of the prostate are reported. Bipedal lymphangiography showed unequivocal involvement of the pelvic nodes in 3 patients and was equivocal in the other 2. Percutaneous transabdominal fine needle aspiration biopsy of abnormal or suspicious iliac nodes was performed and a positive cytolocal diagnosis of metastasis was obtained in all 5 patients. Therefore, staging lymphadenectomy and an extensive radical operation were avoided. Percutaneous fine needle biopsy is a reliable, easy and safe technique that should be used in staging prostatic carcinoma whenever there is evidence of metastatic nodal involvement by lymphangiography.", "contents": "Post-lymphangiography fine needle aspiration lymph node biopsy in staging carcinoma of the prostate: preliminary report. Five cases of clinical stage B carcinoma of the prostate are reported. Bipedal lymphangiography showed unequivocal involvement of the pelvic nodes in 3 patients and was equivocal in the other 2. Percutaneous transabdominal fine needle aspiration biopsy of abnormal or suspicious iliac nodes was performed and a positive cytolocal diagnosis of metastasis was obtained in all 5 patients. Therefore, staging lymphadenectomy and an extensive radical operation were avoided. Percutaneous fine needle biopsy is a reliable, easy and safe technique that should be used in staging prostatic carcinoma whenever there is evidence of metastatic nodal involvement by lymphangiography."}
{"id": "PMID:480493", "title": "Combined serum and bone marrow radioimmunoassays for prostatic acid phosphatase.", "content": "Combined serum and bone marrow radioimmunoassays for prostatic acid phosphatase provide a unique means for the early diagnosis and more accurate clinical staging of prostatic cancer. The combined screening technique appears to be helpful, particularly in providing a clinical assessment of the presence or absence of early, subclinical lymphatic and bone marrow metastases. Low titer elevations of bone marrow prostatic acid phosphatase by radioimmunoassay have been observed commonly in clinically understaged C prostatic cancer with normal technetium bone scans, indicating the presence of unrecognized stage D disease with bone metastases. The combined screening method also is of distinct clinical value in the early diagnosis of prostatic cancer and in monitoring the effects of specific therapy. In therapeutically responsive patients marked suppression of serum and bone marrow prostatic acid phosphatase is observed regularly with the radioimmunochemical method under study.", "contents": "Combined serum and bone marrow radioimmunoassays for prostatic acid phosphatase. Combined serum and bone marrow radioimmunoassays for prostatic acid phosphatase provide a unique means for the early diagnosis and more accurate clinical staging of prostatic cancer. The combined screening technique appears to be helpful, particularly in providing a clinical assessment of the presence or absence of early, subclinical lymphatic and bone marrow metastases. Low titer elevations of bone marrow prostatic acid phosphatase by radioimmunoassay have been observed commonly in clinically understaged C prostatic cancer with normal technetium bone scans, indicating the presence of unrecognized stage D disease with bone metastases. The combined screening method also is of distinct clinical value in the early diagnosis of prostatic cancer and in monitoring the effects of specific therapy. In therapeutically responsive patients marked suppression of serum and bone marrow prostatic acid phosphatase is observed regularly with the radioimmunochemical method under study."}
{"id": "PMID:480494", "title": "Continuous intercostal nerve block for pain relief after lumbar incision.", "content": "To relieve postoperative pain along a lumbar incision in 9 patients the intercostal nerves were blocked with catheters for continuous epidural anesthesia. The catheters were inserted near the intercostal nerves, above and beneath the incision, just before the wound was closed and 0.25% bupivacaine hydrochloride solution was infused periodically through the catheters. With this technique 5 of 9 patients had a satisfactory analgesic effect and could breathe deeply or cough without pain. The other 4 patients did not have satisfactory results and this was believed to be owing to inadequate insertion of the catheters. None of the patients had any complications. The technique is simple and can produce an analgesic effect repeatedly without causing pain for the patient.", "contents": "Continuous intercostal nerve block for pain relief after lumbar incision. To relieve postoperative pain along a lumbar incision in 9 patients the intercostal nerves were blocked with catheters for continuous epidural anesthesia. The catheters were inserted near the intercostal nerves, above and beneath the incision, just before the wound was closed and 0.25% bupivacaine hydrochloride solution was infused periodically through the catheters. With this technique 5 of 9 patients had a satisfactory analgesic effect and could breathe deeply or cough without pain. The other 4 patients did not have satisfactory results and this was believed to be owing to inadequate insertion of the catheters. None of the patients had any complications. The technique is simple and can produce an analgesic effect repeatedly without causing pain for the patient."}
{"id": "PMID:480495", "title": "Venous injury in major urological surgery.", "content": "With the increasing use of pelvic node dissection for staging and treating pelvic urological cancer injury to the common, external and internal iliac veins will occur. The sequelae of these injuries are described and the treatment is outlined. A case is described that epitomizes the problems and solutions of this condition.", "contents": "Venous injury in major urological surgery. With the increasing use of pelvic node dissection for staging and treating pelvic urological cancer injury to the common, external and internal iliac veins will occur. The sequelae of these injuries are described and the treatment is outlined. A case is described that epitomizes the problems and solutions of this condition."}
{"id": "PMID:480496", "title": "Carbon dioxide uroflowmetry.", "content": "A method using pneumatic compression to determine flow rate is described. The procedure is best suited for physicians using carbon dioxide instrumentation for a urodynamic assessment of patients since the carbon dioxide cystometer is readily converted to a uroflowmeter. Values obtained with this method are similar to values reported in the literature using other techniques. When voided volumes are more than 200 ml. the values for peak flow rate are reproducible within subjects and are volume-dependent.", "contents": "Carbon dioxide uroflowmetry. A method using pneumatic compression to determine flow rate is described. The procedure is best suited for physicians using carbon dioxide instrumentation for a urodynamic assessment of patients since the carbon dioxide cystometer is readily converted to a uroflowmeter. Values obtained with this method are similar to values reported in the literature using other techniques. When voided volumes are more than 200 ml. the values for peak flow rate are reproducible within subjects and are volume-dependent."}
{"id": "PMID:480497", "title": "Pediatric urodynamics: a clinical comparison of surface versus needle pelvic floor/external sphincter electromyography.", "content": "Urodynamic evaluations were done on 37 children to diagnose voiding pattern abnormalities and/or recurrent urinary infections. Each of 25 children had 2 sets of testing to judge a practical method of urodynamic evaluation. Bipolar anal skin electrodes were compared to bipolar perianal muscle needle electrodes as a means of monitoring the urethral sphincter/pelvic floow electromyographic activity. In addition, the urethral catheter was compared to the suprapubic catheter as a means of monitoring intravesical pressure. The results were similar and statistically significant (p less than 0.001). The remaining 12 children were evaluated based only on the results of bipolar anal skin electrodes and uroflowmetry. The results of both groups clearly demonstrated that surface perianal electrodes are practical, accurate and reliable for the diagnosis and treatment of children with voiding pattern abnormalities. We recommend the use of surface electrodes and a urethral catheter as techniques for the urodynamic evaluation of voiding pattern abnormalities of children without overt neuropathology or extensive urethral operation. Preoperative surface electromyography of the urinary sphincters may prove to be a useful screening test to detect occult dyssynergia in patients who have had failed ureteral reimplants.", "contents": "Pediatric urodynamics: a clinical comparison of surface versus needle pelvic floor/external sphincter electromyography. Urodynamic evaluations were done on 37 children to diagnose voiding pattern abnormalities and/or recurrent urinary infections. Each of 25 children had 2 sets of testing to judge a practical method of urodynamic evaluation. Bipolar anal skin electrodes were compared to bipolar perianal muscle needle electrodes as a means of monitoring the urethral sphincter/pelvic floow electromyographic activity. In addition, the urethral catheter was compared to the suprapubic catheter as a means of monitoring intravesical pressure. The results were similar and statistically significant (p less than 0.001). The remaining 12 children were evaluated based only on the results of bipolar anal skin electrodes and uroflowmetry. The results of both groups clearly demonstrated that surface perianal electrodes are practical, accurate and reliable for the diagnosis and treatment of children with voiding pattern abnormalities. We recommend the use of surface electrodes and a urethral catheter as techniques for the urodynamic evaluation of voiding pattern abnormalities of children without overt neuropathology or extensive urethral operation. Preoperative surface electromyography of the urinary sphincters may prove to be a useful screening test to detect occult dyssynergia in patients who have had failed ureteral reimplants."}
{"id": "PMID:480498", "title": "Increased physical growth after successful antireflux operation.", "content": "Gross vesicoureteral reflux with infection can retard renal growth. Resumption of renal growth or even accelerated growth has been reported after successful surgical repair of reflux in children. No published reports examining the effect on physical growth are available. The preoperative and postoperative physical growth curves were examined in 35 consecutive pediatric patients undergoing ureteroneocystostomy for reflux. The significant (average 81%) increase in the postoperative physical growth percentile is presented and discussed.", "contents": "Increased physical growth after successful antireflux operation. Gross vesicoureteral reflux with infection can retard renal growth. Resumption of renal growth or even accelerated growth has been reported after successful surgical repair of reflux in children. No published reports examining the effect on physical growth are available. The preoperative and postoperative physical growth curves were examined in 35 consecutive pediatric patients undergoing ureteroneocystostomy for reflux. The significant (average 81%) increase in the postoperative physical growth percentile is presented and discussed."}
{"id": "PMID:480499", "title": "Bacteriuria with intestinal loop urinary diversion in children.", "content": "Bacteriuria and intestinal loop urinary diversion with open (refluxing) ureterointestinal anastomoses did not prevent normal renal and body growth in 44 children, who were followed for 55.2 months. A comparison of the long-term use of sulfamethoxazole and nitrofurantoin with no treatment showed no statistically significant changes in the stoma, loop residual, renal function, serum electrolytes and treatment or prevention of bacteriuria.", "contents": "Bacteriuria with intestinal loop urinary diversion in children. Bacteriuria and intestinal loop urinary diversion with open (refluxing) ureterointestinal anastomoses did not prevent normal renal and body growth in 44 children, who were followed for 55.2 months. A comparison of the long-term use of sulfamethoxazole and nitrofurantoin with no treatment showed no statistically significant changes in the stoma, loop residual, renal function, serum electrolytes and treatment or prevention of bacteriuria."}
{"id": "PMID:480500", "title": "Prepubertal gonorrhea.", "content": "Two cases of gonococcal urethritis in male children are presented and the records of 45 other children with gonorrhea are reviewed. The presentation, diagnosis and treatment are discussed. Data on the mode of transmission are presented with emphasis on the high incidence of gonorrhea in family members or close contacts of infected children, or a history of sexual abuse.", "contents": "Prepubertal gonorrhea. Two cases of gonococcal urethritis in male children are presented and the records of 45 other children with gonorrhea are reviewed. The presentation, diagnosis and treatment are discussed. Data on the mode of transmission are presented with emphasis on the high incidence of gonorrhea in family members or close contacts of infected children, or a history of sexual abuse."}
{"id": "PMID:480503", "title": "Enucleative surgery for renal malignancy.", "content": "Bilateral renal cell carcinoma, malignant tumors of a solitary kidney or renal carcinoma in the presence of a contralateral kidney with compromised function may be treated by simple enucleation of the tumor, usually pseudoencapsulated, permitting relatively avascular removal. We treated 5 such patients by surgical enucleation of renal cell carcinoma who have survived for intervals of 9 months to 5 years without evidence of recurrent local or metastic disease. The simplicity of the technique offers several advantages, primarily those of applicability to lesions of any portion of the kidney and the minimal disturbance of renal hemodynamics and renal function.", "contents": "Enucleative surgery for renal malignancy. Bilateral renal cell carcinoma, malignant tumors of a solitary kidney or renal carcinoma in the presence of a contralateral kidney with compromised function may be treated by simple enucleation of the tumor, usually pseudoencapsulated, permitting relatively avascular removal. We treated 5 such patients by surgical enucleation of renal cell carcinoma who have survived for intervals of 9 months to 5 years without evidence of recurrent local or metastic disease. The simplicity of the technique offers several advantages, primarily those of applicability to lesions of any portion of the kidney and the minimal disturbance of renal hemodynamics and renal function."}
{"id": "PMID:480504", "title": "Revision of ureteral reimplantation by the transverse advancement technique.", "content": "Antireflux ureteral reimplantation may be performed by a variety of techniques with consistently satisfactory results. Complications, such as persistent reflux and postoperative ureterovesical obstruction, may be encountered occasionally and, when significant, they will necessitate a revision operation. Herein 6 cases are described of failed ureteral reimplantation, which were revised successfully by performing a transverse advancement ureteral reimplantation.", "contents": "Revision of ureteral reimplantation by the transverse advancement technique. Antireflux ureteral reimplantation may be performed by a variety of techniques with consistently satisfactory results. Complications, such as persistent reflux and postoperative ureterovesical obstruction, may be encountered occasionally and, when significant, they will necessitate a revision operation. Herein 6 cases are described of failed ureteral reimplantation, which were revised successfully by performing a transverse advancement ureteral reimplantation."}
{"id": "PMID:480505", "title": "Obstructive uropathy and nephrogenic diabetes insipidus in infants.", "content": "The syndrome of acute post-obstructive nephrogenic diabetes insipidus is a rare phenomenon. The lesion is acquired during the pre-diuretic phase, owing to antidiuretic hormone resistance of the distal tubule as well as a severe concentrating defect. The diuretic phase after relief of obstruction can result in a massive, sustained and life-threatening diuresis. Sodium restriction and thiazide diuretics produce a mild volume contracted state, enhancing sodium and water reabsorption, primarily in the proximal tubule and possibly in the distal tubule owing to aldosterone. The recognition and differentiation of this unique pyloric syndrome from other more common post-obstructive diuretic states are important for all urologists who are responsible for the care of children.", "contents": "Obstructive uropathy and nephrogenic diabetes insipidus in infants. The syndrome of acute post-obstructive nephrogenic diabetes insipidus is a rare phenomenon. The lesion is acquired during the pre-diuretic phase, owing to antidiuretic hormone resistance of the distal tubule as well as a severe concentrating defect. The diuretic phase after relief of obstruction can result in a massive, sustained and life-threatening diuresis. Sodium restriction and thiazide diuretics produce a mild volume contracted state, enhancing sodium and water reabsorption, primarily in the proximal tubule and possibly in the distal tubule owing to aldosterone. The recognition and differentiation of this unique pyloric syndrome from other more common post-obstructive diuretic states are important for all urologists who are responsible for the care of children."}
{"id": "PMID:480507", "title": "Retroperitoneal seminoma presenting with inferior vena caval obstruction.", "content": "A retroperitoneal seminoma in the presence of clinically normal testes can present a considerable diagnostic problem and therapeutic dilemma. We report on a patient with low back pain and inferior vena caval obstruction in whom no primary neoplasm could be detected by surgical exploration of the testes.", "contents": "Retroperitoneal seminoma presenting with inferior vena caval obstruction. A retroperitoneal seminoma in the presence of clinically normal testes can present a considerable diagnostic problem and therapeutic dilemma. We report on a patient with low back pain and inferior vena caval obstruction in whom no primary neoplasm could be detected by surgical exploration of the testes."}
{"id": "PMID:480508", "title": "Computed tomography in evolution of testicular cancer during intensive chemotherapy.", "content": "The evolution of malignant testicular tumor to mature teratoma has been studied in 4 patients. Computed tomography has been helpful in early diagnosis of this biologic phenomenon in these patients receiving intensive chemotherapy for disseminated non-seminomatous testicular cancer. Although the potential significance of this conversion in terms of survival is not known its early recognition by computed tomography has been useful in selecting and monitoring the therapy of these patients.", "contents": "Computed tomography in evolution of testicular cancer during intensive chemotherapy. The evolution of malignant testicular tumor to mature teratoma has been studied in 4 patients. Computed tomography has been helpful in early diagnosis of this biologic phenomenon in these patients receiving intensive chemotherapy for disseminated non-seminomatous testicular cancer. Although the potential significance of this conversion in terms of survival is not known its early recognition by computed tomography has been useful in selecting and monitoring the therapy of these patients."}
{"id": "PMID:480509", "title": "Urogenital sinus abnormalities in the female patient.", "content": "Three patients with a urogenital sinus and 1 with a cloaca presented with complex diagnostic and therapeutic anomalies. A classification of these and other vaginal anomalies is based on the theory that they all represent an arrest in normal embryological development. Six categories are described, including vaginal agenesis, urogenital sinus, vaginal atresia, vaginal septa, vaginal stenosis and cloaca.", "contents": "Urogenital sinus abnormalities in the female patient. Three patients with a urogenital sinus and 1 with a cloaca presented with complex diagnostic and therapeutic anomalies. A classification of these and other vaginal anomalies is based on the theory that they all represent an arrest in normal embryological development. Six categories are described, including vaginal agenesis, urogenital sinus, vaginal atresia, vaginal septa, vaginal stenosis and cloaca."}
{"id": "PMID:480512", "title": "Paratuberculosis (Johne's disease) in bighorn sheep and a Rocky Mountain goat in Colorado.", "content": "Between May, 1972 and February, 1978, six cases of paratuberculosis (Johne's Disease) caused by Mycobacterium paratuberculosis were diagnosed in free-ranging Rocky Mountain bighorn sheep (Ovis canadensis) and one Rocky Mountain goat (Oreamnos americanus) on or near Mt. Evans in Colorado. Diagnosis of paratuberculosis was based on gross and histopathologic examination of the animals and by isolation of M. paratuberculosis from three sheep and the goat. The clinical signs and pathologic changes seen in the bighorn sheep resembled those described in cattle, while the lesions in the goat were similar to those described for domestic sheep and goats.", "contents": "Paratuberculosis (Johne's disease) in bighorn sheep and a Rocky Mountain goat in Colorado. Between May, 1972 and February, 1978, six cases of paratuberculosis (Johne's Disease) caused by Mycobacterium paratuberculosis were diagnosed in free-ranging Rocky Mountain bighorn sheep (Ovis canadensis) and one Rocky Mountain goat (Oreamnos americanus) on or near Mt. Evans in Colorado. Diagnosis of paratuberculosis was based on gross and histopathologic examination of the animals and by isolation of M. paratuberculosis from three sheep and the goat. The clinical signs and pathologic changes seen in the bighorn sheep resembled those described in cattle, while the lesions in the goat were similar to those described for domestic sheep and goats."}
{"id": "PMID:480513", "title": "Mycobacterium intracellulare infection in a water monitor.", "content": "Mycobacteriosis caused by Mycobacterium intracellulare serotypes Davis (8) and Altman (18) is described in a water monitor (Varanus semiremex). Infection with this of organism has not been reported previously in reptiles in Australia.", "contents": "Mycobacterium intracellulare infection in a water monitor. Mycobacteriosis caused by Mycobacterium intracellulare serotypes Davis (8) and Altman (18) is described in a water monitor (Varanus semiremex). Infection with this of organism has not been reported previously in reptiles in Australia."}
{"id": "PMID:480514", "title": "Isolation of Aeromonas hydrophila from the American alligator, Alligator mississippiensis.", "content": "Aeromonas hydrophila was isolated from the internal organs of nine adult alligators, Alligator mississippiensis, which died without apparent cause, suggesting the bacterium may have been a factor. One hundred and twenty-three alligators ranging in age from six months to over 10 years were captured from five locations in the southeastern United States and sampled for A. hydrophila. The bacterium was isolated from the oral cavity of 85% of the animals, on the external jaw area from over 50% and from 70% of the internal tissue samples. A. hydrophila is ubiquitous with alligators in their natural habitats, but apparently does not cause clinical disease. However, stress factors such as trapping, handling, and warm water temperatures may be conducive to the rapid proliferation of the bacteria, thereby facilitating disease.", "contents": "Isolation of Aeromonas hydrophila from the American alligator, Alligator mississippiensis. Aeromonas hydrophila was isolated from the internal organs of nine adult alligators, Alligator mississippiensis, which died without apparent cause, suggesting the bacterium may have been a factor. One hundred and twenty-three alligators ranging in age from six months to over 10 years were captured from five locations in the southeastern United States and sampled for A. hydrophila. The bacterium was isolated from the oral cavity of 85% of the animals, on the external jaw area from over 50% and from 70% of the internal tissue samples. A. hydrophila is ubiquitous with alligators in their natural habitats, but apparently does not cause clinical disease. However, stress factors such as trapping, handling, and warm water temperatures may be conducive to the rapid proliferation of the bacteria, thereby facilitating disease."}
{"id": "PMID:480515", "title": "Serologic evidence of leptospirosis in woodchucks (Marmota monax) in central New York State.", "content": "Serum samples from 153 woodchucks (Marmota monax) from Tompkins County, New York, obtained in 1976 and 1977, were examined by plate agglutination tests for antibodies against five Leptospira antigens. Fourteen sera showed significant titers against either L. hardjo, L. icterohemorrhagiae and/or L. pomona. Reactions against L. hardjo were the most frequent. Woodchucks collected from two dairy farms with histories of bovine leptospirosis did not have a greater prevalence of antibodies than woodchucks collected from other locations. Each of two woodchucks experimentally-inoculated with L. hardjo developed titers to L. hardjo. Maximum titers occurred approximately 30 days post-inoculation. L. hardjo was not observed in urine specimens of these animals.", "contents": "Serologic evidence of leptospirosis in woodchucks (Marmota monax) in central New York State. Serum samples from 153 woodchucks (Marmota monax) from Tompkins County, New York, obtained in 1976 and 1977, were examined by plate agglutination tests for antibodies against five Leptospira antigens. Fourteen sera showed significant titers against either L. hardjo, L. icterohemorrhagiae and/or L. pomona. Reactions against L. hardjo were the most frequent. Woodchucks collected from two dairy farms with histories of bovine leptospirosis did not have a greater prevalence of antibodies than woodchucks collected from other locations. Each of two woodchucks experimentally-inoculated with L. hardjo developed titers to L. hardjo. Maximum titers occurred approximately 30 days post-inoculation. L. hardjo was not observed in urine specimens of these animals."}
{"id": "PMID:480516", "title": "Use of Ketamine hydrochloride and xylazine hydrochloride to immobilize black bears (Ursus americanus).", "content": "Ketamine hydrochloride (KH) and xylazine hydrochloride (XH) used in combination (KH-XH) were effective immobilants for captive and wild black bears (Ursus americanus). Single intramuscular injections of 1.5-17.1 mg of KH per kg body weight combined in an approximate ratio of 2:1 with 0.9-10.0 mg of XH per kg body weight immobilized bears for 1.5-197 min. Dosages most frequently used were 4.5-9 mg KH/kg with 2-4.5 mg XH kg. Supplemental administrations maintained tractability for up to 31 h. Immobilization was characterized by smooth induction, relaxed muscles, occasional groaning and vomition, no eye closure, no defecation, and a smooth recovery phase of variable length. Male and female bears responded similarly to KH-XH. Induction times for small bears (less than or equal to 25 kg) were shorter than for larger bears.", "contents": "Use of Ketamine hydrochloride and xylazine hydrochloride to immobilize black bears (Ursus americanus). Ketamine hydrochloride (KH) and xylazine hydrochloride (XH) used in combination (KH-XH) were effective immobilants for captive and wild black bears (Ursus americanus). Single intramuscular injections of 1.5-17.1 mg of KH per kg body weight combined in an approximate ratio of 2:1 with 0.9-10.0 mg of XH per kg body weight immobilized bears for 1.5-197 min. Dosages most frequently used were 4.5-9 mg KH/kg with 2-4.5 mg XH kg. Supplemental administrations maintained tractability for up to 31 h. Immobilization was characterized by smooth induction, relaxed muscles, occasional groaning and vomition, no eye closure, no defecation, and a smooth recovery phase of variable length. Male and female bears responded similarly to KH-XH. Induction times for small bears (less than or equal to 25 kg) were shorter than for larger bears."}
{"id": "PMID:480517", "title": "Gastrointestinal nematodes of wild sheep (Ovis orientalis) from Iran.", "content": "A total of 250 wild sheep (Ovis orientalis) from different national parks and protected regions of Iran were examined for gastrointestinal nematodes at necropsy. Twenty five species of nematodes were found. Marshallagia marshalli, Ostertagia spp; Nematodirus spp; and Skrjabinema ovis were the most prevalent. Although all the species found are recorded from wild sheep for the first time in Iran, 88% were reported previously from domestic sheep. New host and distribution records for Nematodirus davtiani, N. gazellae and Nematodirella longissimespiculata were established during the present study.", "contents": "Gastrointestinal nematodes of wild sheep (Ovis orientalis) from Iran. A total of 250 wild sheep (Ovis orientalis) from different national parks and protected regions of Iran were examined for gastrointestinal nematodes at necropsy. Twenty five species of nematodes were found. Marshallagia marshalli, Ostertagia spp; Nematodirus spp; and Skrjabinema ovis were the most prevalent. Although all the species found are recorded from wild sheep for the first time in Iran, 88% were reported previously from domestic sheep. New host and distribution records for Nematodirus davtiani, N. gazellae and Nematodirella longissimespiculata were established during the present study."}
{"id": "PMID:480518", "title": "Tick paralysis in a grey fox.", "content": "One hundred seventeen Dermacentor variabilis were removed from the head and back of a grey fox (Urocyon cinereoargenteus) showing paresis and diminished motor reflexes of the hind limbs. Rapid and total recovery led to a diagnosis of tick paralysis.", "contents": "Tick paralysis in a grey fox. One hundred seventeen Dermacentor variabilis were removed from the head and back of a grey fox (Urocyon cinereoargenteus) showing paresis and diminished motor reflexes of the hind limbs. Rapid and total recovery led to a diagnosis of tick paralysis."}
{"id": "PMID:480519", "title": "Dermacentor albipictus on moose (Alces alces) in Ontario.", "content": "Fifty-five moose (Alces alces) collected from 1963 to 1965 in the Chapleau Crown Game Preserve of northern Ontario were examined for ectoparasites. Dermacentor albipictus was the only parasite recovered. D. albipictus was absent from 15 moose examined during June, July and August; first observed on 1 or 2 moose examined in September; and present on all 38 moose collected from October to May. Ticks were not evenly distributed on the body. Infestations varied from 32 to 13,490 ticks/moose for 14 individuals. Levels of infestation observed did not have an obvious adverse effect on the health of the moose.", "contents": "Dermacentor albipictus on moose (Alces alces) in Ontario. Fifty-five moose (Alces alces) collected from 1963 to 1965 in the Chapleau Crown Game Preserve of northern Ontario were examined for ectoparasites. Dermacentor albipictus was the only parasite recovered. D. albipictus was absent from 15 moose examined during June, July and August; first observed on 1 or 2 moose examined in September; and present on all 38 moose collected from October to May. Ticks were not evenly distributed on the body. Infestations varied from 32 to 13,490 ticks/moose for 14 individuals. Levels of infestation observed did not have an obvious adverse effect on the health of the moose."}
{"id": "PMID:480520", "title": "Tonsillar carcinoma with metastases in a captive wolf.", "content": "Gross and histopathologic findings of primary tonsillar squamous carcinoma with metastases to lymph nodes of the neck and thorax and to the lung in a captive 13-year-old male wolf are presented.", "contents": "Tonsillar carcinoma with metastases in a captive wolf. Gross and histopathologic findings of primary tonsillar squamous carcinoma with metastases to lymph nodes of the neck and thorax and to the lung in a captive 13-year-old male wolf are presented."}
{"id": "PMID:480522", "title": "Myxosarcoma in a wapiti.", "content": "Myxosarcoma was diagnosed in an adult male wapiti (Cervus canadensis) from western Colorado. The neoplasm was located on the medial aspect of the right thigh and had extensively infiltrated adjacent soft tissue.", "contents": "Myxosarcoma in a wapiti. Myxosarcoma was diagnosed in an adult male wapiti (Cervus canadensis) from western Colorado. The neoplasm was located on the medial aspect of the right thigh and had extensively infiltrated adjacent soft tissue."}
{"id": "PMID:480523", "title": "Experiments on direct and secondary poisoning by fluoroacetamide (1081) in wildlife and domestic carnivores.", "content": "Fluoroacetamide (1081 or F.A.A) is used in Israel for field rodent control. Experiments on direct and secondary, short and long term poisoning caused by 1081 were carried out. Mongoose (Herpestes ichneumon), hyena (Hyaena hyaena), cats and dogs were susceptible. Barn owls (Tyto alba), buzzards (Buteo buteo) and black kites (Milvus m. migrans) were markedly resistant. Barn owls tolerated total direct poisoning ranging from 6.8 to 10.9, and a final dose ranging from 0.8 to 2.0 mg/kg. In secondary poisoning, total doses ranging from 1.7 to 7.1, and final doses ranging from 0.2 to 1.3 mg/kg were tolerated. Buzzards tolerated total direct poisoning ranging from 6 to 12.0, and final doses ranging from 0.7 to 1.3 mg/kg. In secondary poisoning, doses ranging from 0.8 to 10.3 and final doses ranging from 0.2 to 2.4 mg/kg were tolerated. One black kite tolerated a total direct dose of 6.1 and final dose of 0.7 mg/kg, another survived a total dose of 2.3 and final dose of 0.2 mg/kg in secondary poisoning. A small-scale secondary poisoning experiment on two Palestine vipers (Vipera palestinae), a Syrian black snake (Coluber jugularis) and two Montpellier snakes (Malpolon monspessulanus) indicated that these species were resistant to total doses ranging from 0.1 to 3.2 and final doses of 0.1 to 0.8 mg/kg.", "contents": "Experiments on direct and secondary poisoning by fluoroacetamide (1081) in wildlife and domestic carnivores. Fluoroacetamide (1081 or F.A.A) is used in Israel for field rodent control. Experiments on direct and secondary, short and long term poisoning caused by 1081 were carried out. Mongoose (Herpestes ichneumon), hyena (Hyaena hyaena), cats and dogs were susceptible. Barn owls (Tyto alba), buzzards (Buteo buteo) and black kites (Milvus m. migrans) were markedly resistant. Barn owls tolerated total direct poisoning ranging from 6.8 to 10.9, and a final dose ranging from 0.8 to 2.0 mg/kg. In secondary poisoning, total doses ranging from 1.7 to 7.1, and final doses ranging from 0.2 to 1.3 mg/kg were tolerated. Buzzards tolerated total direct poisoning ranging from 6 to 12.0, and final doses ranging from 0.7 to 1.3 mg/kg. In secondary poisoning, doses ranging from 0.8 to 10.3 and final doses ranging from 0.2 to 2.4 mg/kg were tolerated. One black kite tolerated a total direct dose of 6.1 and final dose of 0.7 mg/kg, another survived a total dose of 2.3 and final dose of 0.2 mg/kg in secondary poisoning. A small-scale secondary poisoning experiment on two Palestine vipers (Vipera palestinae), a Syrian black snake (Coluber jugularis) and two Montpellier snakes (Malpolon monspessulanus) indicated that these species were resistant to total doses ranging from 0.1 to 3.2 and final doses of 0.1 to 0.8 mg/kg."}
{"id": "PMID:480524", "title": "Prevalence of selected zoonotic diseases in vertebrates from Haiti, 1972.", "content": "Vertebrate animals collected in Haiti in 1972 were tested for selected zoonotic diseases. No rabies virus or neutralizing (N) antibody was detected in bats (Artibeus jamaicensis). However, N antibody against St. Louis encephalitis, Western equine encephalitis (WEE), and Eastern equine encephalitis were detected in resident species of birds and WEE antibody in bats. No N antibody against Venezuelan equine encephalitis was found. The possible introduction by migratory birds and local transmission of these arboviruses is discussed.", "contents": "Prevalence of selected zoonotic diseases in vertebrates from Haiti, 1972. Vertebrate animals collected in Haiti in 1972 were tested for selected zoonotic diseases. No rabies virus or neutralizing (N) antibody was detected in bats (Artibeus jamaicensis). However, N antibody against St. Louis encephalitis, Western equine encephalitis (WEE), and Eastern equine encephalitis were detected in resident species of birds and WEE antibody in bats. No N antibody against Venezuelan equine encephalitis was found. The possible introduction by migratory birds and local transmission of these arboviruses is discussed."}
{"id": "PMID:480525", "title": "Isolation of Newcastle disease virus from teals (Anas crecca) in Iran.", "content": "Eight of 30 teals (Anas crecca) died several days following capture and Newcastle Disease Virus (NDV) was isolated from all eight. Brains from the dead birds were homogenized and inoculated into chicken embryos. The allantoic fluid from the embryos were inoculated into 10 domestic chickens susceptible to NDV and 10 chickens immunized against NDV. Eight of 10 (80%) susceptible chickens died, while the immunized chickens remained healthy. Anti-NDV serum showed complete homology against NDV and the eight isolates.", "contents": "Isolation of Newcastle disease virus from teals (Anas crecca) in Iran. Eight of 30 teals (Anas crecca) died several days following capture and Newcastle Disease Virus (NDV) was isolated from all eight. Brains from the dead birds were homogenized and inoculated into chicken embryos. The allantoic fluid from the embryos were inoculated into 10 domestic chickens susceptible to NDV and 10 chickens immunized against NDV. Eight of 10 (80%) susceptible chickens died, while the immunized chickens remained healthy. Anti-NDV serum showed complete homology against NDV and the eight isolates."}
{"id": "PMID:480537", "title": "Additional data favoring use of anticoagulant therapy in myocardial infarction. A population-based study.", "content": "A community-wide study was conducted in metropolitan Baltimore in which the survival of 1,307 patients with acute myocardial infarction was examined according to use of anticoagulants. The adjusted in-hospital case-fatality rate was lower for patients receiving anticoagulants (18%) than for those not receiving this therapy (31%). This difference persisted in each period examined in the study (1966 and 1967 or 1971) and was found in 17 of 20 participating hospitals. For hospital survivors followed up for as long as ten years, a better survival was again found for those treated with anticoagulants in the acute phase compared with those not treated.", "contents": "Additional data favoring use of anticoagulant therapy in myocardial infarction. A population-based study. A community-wide study was conducted in metropolitan Baltimore in which the survival of 1,307 patients with acute myocardial infarction was examined according to use of anticoagulants. The adjusted in-hospital case-fatality rate was lower for patients receiving anticoagulants (18%) than for those not receiving this therapy (31%). This difference persisted in each period examined in the study (1966 and 1967 or 1971) and was found in 17 of 20 participating hospitals. For hospital survivors followed up for as long as ten years, a better survival was again found for those treated with anticoagulants in the acute phase compared with those not treated."}
{"id": "PMID:480538", "title": "Death during jogging or running. A study of 18 cases.", "content": "We investigated the circumstances of death and the medical and activity histories of 18 individuals who died during or immediately after jogging. Thirteen men died of coronary heart disease (CHD) and four men and one woman died of other causes. Six CHD subjects had medical histories relevant to the cardiovascular system, but only one had diagnosed CHD. Six CHD subjects experienced prodromal symptoms but continued vigorous exercise programs. Two subjects had exercised less than a month, but most had trained regularly for years. The CHD risk factors for the CHD cases did not differ significantly from those for other age-matched, physically active men. Superior physical fitness does not guarantee protection against exercise deaths. Physicians and exercising adults should be aware of this fact and give appropriate attention to possible prodromal symptoms.", "contents": "Death during jogging or running. A study of 18 cases. We investigated the circumstances of death and the medical and activity histories of 18 individuals who died during or immediately after jogging. Thirteen men died of coronary heart disease (CHD) and four men and one woman died of other causes. Six CHD subjects had medical histories relevant to the cardiovascular system, but only one had diagnosed CHD. Six CHD subjects experienced prodromal symptoms but continued vigorous exercise programs. Two subjects had exercised less than a month, but most had trained regularly for years. The CHD risk factors for the CHD cases did not differ significantly from those for other age-matched, physically active men. Superior physical fitness does not guarantee protection against exercise deaths. Physicians and exercising adults should be aware of this fact and give appropriate attention to possible prodromal symptoms."}
{"id": "PMID:480539", "title": "Family history in an oncology clinic. Implications for cancer genetics.", "content": "Detailed family histories of cancer were solicited from 200 consecutively ascertained cancer patients undergoing treatment in an oncology clinic. Approximately 18% had two or more first-degree relatives with cancer of any anatomic site. In several cases, striking familial aggregations of cancer fulfilled more rigorous criteria for hereditary cancer syndromes, including early age at onset of generally late-occurring tumors, characteristic tumor patterns, vertical transmission, and collateral family lines similarly afflicted. Review of preexisting clinic charts demonstrated that, in most cases, the family history of cancer had been either omitted altogether, reported as negative despite substantial evidence to the contrary, or, if noted as positive, not pursued or acted on. Family history can be more successfully utilized in recognition of suggestive familial cancer aggregations, ultimate identification of hereditary cancer syndromes, and control of cancer in clinical practice.", "contents": "Family history in an oncology clinic. Implications for cancer genetics. Detailed family histories of cancer were solicited from 200 consecutively ascertained cancer patients undergoing treatment in an oncology clinic. Approximately 18% had two or more first-degree relatives with cancer of any anatomic site. In several cases, striking familial aggregations of cancer fulfilled more rigorous criteria for hereditary cancer syndromes, including early age at onset of generally late-occurring tumors, characteristic tumor patterns, vertical transmission, and collateral family lines similarly afflicted. Review of preexisting clinic charts demonstrated that, in most cases, the family history of cancer had been either omitted altogether, reported as negative despite substantial evidence to the contrary, or, if noted as positive, not pursued or acted on. Family history can be more successfully utilized in recognition of suggestive familial cancer aggregations, ultimate identification of hereditary cancer syndromes, and control of cancer in clinical practice."}
{"id": "PMID:480540", "title": "Drug-related superinfection in hospitalized patients.", "content": "In a series of 14,077 hospitalized medical patients receiving antibiotics, superinfection developed in 95 (0.7%) during drug therapy. The majority were yeast and fungal infections, and serious infections occurred with a frequency of less than one per 1,000 patients treated. Concurrent immunosuppression and impaired renal function increased the risk of new infections.", "contents": "Drug-related superinfection in hospitalized patients. In a series of 14,077 hospitalized medical patients receiving antibiotics, superinfection developed in 95 (0.7%) during drug therapy. The majority were yeast and fungal infections, and serious infections occurred with a frequency of less than one per 1,000 patients treated. Concurrent immunosuppression and impaired renal function increased the risk of new infections."}
{"id": "PMID:480541", "title": "Contact dermatitis to neomycin sulfate.", "content": "The prevalence of contact allergic dermatitis and nonallergic irritant reactions to neomycin sulfate was determined in a large group of young adults who had a noteworthy exposure to topical antibiotics. Only two subjects (0.09%) had contact allergy proved by closed patch testing, while 20 (0.9%) had nonallergic irritant reactions to 20% neomycin sulfate and 17 (0.78%) had nonallergic reactions to one of the hydrophilic ointment bases. In a separate population of 653 children referred for diagnostic patch testing, only one subject had contact allergy to neomycin. All three subjects who had reactions had a history of repeated use of neomycin on chronically damaged skin. These results support the conclusion that intermittent use of neomycin on minor cuts and wounds is not associated with an excessive rate of sensitization.", "contents": "Contact dermatitis to neomycin sulfate. The prevalence of contact allergic dermatitis and nonallergic irritant reactions to neomycin sulfate was determined in a large group of young adults who had a noteworthy exposure to topical antibiotics. Only two subjects (0.09%) had contact allergy proved by closed patch testing, while 20 (0.9%) had nonallergic irritant reactions to 20% neomycin sulfate and 17 (0.78%) had nonallergic reactions to one of the hydrophilic ointment bases. In a separate population of 653 children referred for diagnostic patch testing, only one subject had contact allergy to neomycin. All three subjects who had reactions had a history of repeated use of neomycin on chronically damaged skin. These results support the conclusion that intermittent use of neomycin on minor cuts and wounds is not associated with an excessive rate of sensitization."}
{"id": "PMID:480542", "title": "Antimicrobial selection by a computer. A blinded evaluation by infectious diseases experts.", "content": "An evaluation of a computer-based consultation system called MYCIN was made. Eight independent evaluators with special expertise in the management of meningitis compared MYCIN's choice of antimicrobials with the choices of nine human prescribers for ten test cases of meningitis. MYCIN received an acceptability rating of 65% by the evaluators; the corresponding ratings for acceptability of the regimen prescribed by the five faculty specialists ranged from 42.5% to 62.5%. The system never failed to cover a treatable pathogen while demonstrating efficiency in minimizing the number of antimicrobials prescribed. The study design may be useful in assessing the performance of other computer-based clinical decision-making systems.", "contents": "Antimicrobial selection by a computer. A blinded evaluation by infectious diseases experts. An evaluation of a computer-based consultation system called MYCIN was made. Eight independent evaluators with special expertise in the management of meningitis compared MYCIN's choice of antimicrobials with the choices of nine human prescribers for ten test cases of meningitis. MYCIN received an acceptability rating of 65% by the evaluators; the corresponding ratings for acceptability of the regimen prescribed by the five faculty specialists ranged from 42.5% to 62.5%. The system never failed to cover a treatable pathogen while demonstrating efficiency in minimizing the number of antimicrobials prescribed. The study design may be useful in assessing the performance of other computer-based clinical decision-making systems."}
{"id": "PMID:480558", "title": "Reye's syndrome in nonpediatric age groups.", "content": "Reye's syndrome (encephalopathy and fatty liver) is generally considered a disease of children. Four patients, aged 16, 18, 19, and 23 years, with Reye's syndrome were initially seen by internists. A viral prodrome followed by vomiting and encephalopathy without focal neurological signs or jaundice clinically suggested Reye's syndrome. Normal findings of CSF examination (except for increased opening pressure), abnormal findings of liver function tests, and increased blood ammonia further supported the diagnosis. None was hypoglycemic. Reye's syndrome was related to influenza B virus in three patients and to Varicella in another. Three patients survived. Reye's syndrome may be seen intially by general practitioners, emergency room physicians, internists, or psychiatrists. The importance of considering this syndrome in the differential diagnosis of unexplained encephalopathy in adults is stressed.", "contents": "Reye's syndrome in nonpediatric age groups. Reye's syndrome (encephalopathy and fatty liver) is generally considered a disease of children. Four patients, aged 16, 18, 19, and 23 years, with Reye's syndrome were initially seen by internists. A viral prodrome followed by vomiting and encephalopathy without focal neurological signs or jaundice clinically suggested Reye's syndrome. Normal findings of CSF examination (except for increased opening pressure), abnormal findings of liver function tests, and increased blood ammonia further supported the diagnosis. None was hypoglycemic. Reye's syndrome was related to influenza B virus in three patients and to Varicella in another. Three patients survived. Reye's syndrome may be seen intially by general practitioners, emergency room physicians, internists, or psychiatrists. The importance of considering this syndrome in the differential diagnosis of unexplained encephalopathy in adults is stressed."}
{"id": "PMID:480559", "title": "Energy levels and patient weight in ventricular defibrillation.", "content": "In a prospective survey employing conventional devices that stored 400 joules or less, direct-current shocks that delivered 194 +/- 11 joules (1.8 joules/kg) to the chest wall terminated 45 of 46 episodes of ventricular fibrillation in 11 of 12 patients weighing 91 to 225 kg. Patients with coronary disease defibrillated more easily than patients without coronary disease. Weight did not determine outcome. The 98% defibrillation efficiency occurred at only half the energy predicted for 50% efficiency from retrospective data. Human defibrillation not only occurred at one third the untested level recommended by proponents of high-energy defibrillation, but also at one fourth the energy, defibrillating 69% of comparably heavy animals. Thus, the costly defibrillators delivering 400 to 800 joules now sold by 11 of 14 American manufacturers are superfluous, untested, potentially lethal devices with which to attempt ventricular defibrillation.", "contents": "Energy levels and patient weight in ventricular defibrillation. In a prospective survey employing conventional devices that stored 400 joules or less, direct-current shocks that delivered 194 +/- 11 joules (1.8 joules/kg) to the chest wall terminated 45 of 46 episodes of ventricular fibrillation in 11 of 12 patients weighing 91 to 225 kg. Patients with coronary disease defibrillated more easily than patients without coronary disease. Weight did not determine outcome. The 98% defibrillation efficiency occurred at only half the energy predicted for 50% efficiency from retrospective data. Human defibrillation not only occurred at one third the untested level recommended by proponents of high-energy defibrillation, but also at one fourth the energy, defibrillating 69% of comparably heavy animals. Thus, the costly defibrillators delivering 400 to 800 joules now sold by 11 of 14 American manufacturers are superfluous, untested, potentially lethal devices with which to attempt ventricular defibrillation."}
{"id": "PMID:480560", "title": "An evaluation of iridology.", "content": "Iridology is an analysis of health based on examination of the iris of the eye. One hundred forty-three patients had photographs taken of both eyes. Nine-five patients were free of kidney disease, defined as a creatinine level of less than 1.2 mg/dL (mean, 0.8 mg/dL), and 48 had kidney disease severe enough to raise the plasma creatinine level to 1.5 mg/dL or greater (mean, 6.5 mg/dL). Three ophthalmologists and three iridologists viewed the slides in a randomized sequence without knowledge of the number of patients in the two categories or any information about patient history. Iridology had no clinical or statistically significant ability to detect the presence of kidney disease. Iridology was neither selective nor specific, and the likelihood of correct detection was statistically no better than chance.", "contents": "An evaluation of iridology. Iridology is an analysis of health based on examination of the iris of the eye. One hundred forty-three patients had photographs taken of both eyes. Nine-five patients were free of kidney disease, defined as a creatinine level of less than 1.2 mg/dL (mean, 0.8 mg/dL), and 48 had kidney disease severe enough to raise the plasma creatinine level to 1.5 mg/dL or greater (mean, 6.5 mg/dL). Three ophthalmologists and three iridologists viewed the slides in a randomized sequence without knowledge of the number of patients in the two categories or any information about patient history. Iridology had no clinical or statistically significant ability to detect the presence of kidney disease. Iridology was neither selective nor specific, and the likelihood of correct detection was statistically no better than chance."}
{"id": "PMID:480562", "title": "Expanding psychiatric service in a hospital emergency room.", "content": "Senior medical residents were used to extend psychiatric coverage in a busy hospital emergency room. Their service was supported by the backup consultation of senior psychiatric physicians. The medical residents worked in conjunction with psychiatric residents to provide 24-hour daily emergency psychiatric service to more than 350 patients during a 12-month period. There were no significant differences in the demographic composition of the patient population seen or in the disposition of individual patients made between the medical and psychiatric residents. The hospital was able to expand its psychiatric service in the emergency room without major increases in cost. The medical residents benefited by experiencing a more diversified patient care training program, and they earned a modest supplement to their houser officer salary.", "contents": "Expanding psychiatric service in a hospital emergency room. Senior medical residents were used to extend psychiatric coverage in a busy hospital emergency room. Their service was supported by the backup consultation of senior psychiatric physicians. The medical residents worked in conjunction with psychiatric residents to provide 24-hour daily emergency psychiatric service to more than 350 patients during a 12-month period. There were no significant differences in the demographic composition of the patient population seen or in the disposition of individual patients made between the medical and psychiatric residents. The hospital was able to expand its psychiatric service in the emergency room without major increases in cost. The medical residents benefited by experiencing a more diversified patient care training program, and they earned a modest supplement to their houser officer salary."}
{"id": "PMID:480579", "title": "Appendectomies during physicians' boycott. Analysis of surgical care.", "content": "The 1975 San Francisco physicians' boycott caused major changes in patterns of providing surgical care, but there was no appreciable change in the number of appendectomies performed during the boycott month when compared with a six-month baseline period. The ratio of normal to inflamed appendices removed was no different during the boycott than it was during the control period, and no differences were noted in the percentage of cases perforating in the boycott month when compared with the control period. In addition, patients seeking care for appendicitis during the boycott month did not experience delays. The lack of change in the standard direct and indirect indicators used to monitor quality of care suggests no major lapses in appendicitis care occurred in San Francisco during the boycott.", "contents": "Appendectomies during physicians' boycott. Analysis of surgical care. The 1975 San Francisco physicians' boycott caused major changes in patterns of providing surgical care, but there was no appreciable change in the number of appendectomies performed during the boycott month when compared with a six-month baseline period. The ratio of normal to inflamed appendices removed was no different during the boycott than it was during the control period, and no differences were noted in the percentage of cases perforating in the boycott month when compared with the control period. In addition, patients seeking care for appendicitis during the boycott month did not experience delays. The lack of change in the standard direct and indirect indicators used to monitor quality of care suggests no major lapses in appendicitis care occurred in San Francisco during the boycott."}
{"id": "PMID:480580", "title": "Aspergillosis and other systemic mycoses. The growing problem.", "content": "To measure the incidence in the United States of systemic mycoses necessitating hospitalization, we reviewed discharge records of 1,875 hospitals participating in the Professional Activity Study of the Commission on Professional and Hospital Activities. Projected incidence rates in 1976 ranged from 23.0 per million for histoplasmosis to 0.2 per million for blastomycosis. High prevalences of leukemia or lymphoma (5.9% to 10.2%) or of other malignancies (9.9% to 13.2%) were recorded in patients with aspergillosis, candidasis, or cryptococcosis. High prevalences of chronic obstructive lung disease (9.6% to 9.9%) were recorded in those with aspergillosis or histoplasmosis. Marked increases from 1970 to 1976 were found in the incidence of aspergillosis (158%), actinomycosis (92%), cryptococcosis (78%), and coccidioidomycosis (74%). Increasing numbers of persons with immunosuppressive conditions, migration of susceptible persons into hyperendemic areas, and aging of the population contributed to the increases.", "contents": "Aspergillosis and other systemic mycoses. The growing problem. To measure the incidence in the United States of systemic mycoses necessitating hospitalization, we reviewed discharge records of 1,875 hospitals participating in the Professional Activity Study of the Commission on Professional and Hospital Activities. Projected incidence rates in 1976 ranged from 23.0 per million for histoplasmosis to 0.2 per million for blastomycosis. High prevalences of leukemia or lymphoma (5.9% to 10.2%) or of other malignancies (9.9% to 13.2%) were recorded in patients with aspergillosis, candidasis, or cryptococcosis. High prevalences of chronic obstructive lung disease (9.6% to 9.9%) were recorded in those with aspergillosis or histoplasmosis. Marked increases from 1970 to 1976 were found in the incidence of aspergillosis (158%), actinomycosis (92%), cryptococcosis (78%), and coccidioidomycosis (74%). Increasing numbers of persons with immunosuppressive conditions, migration of susceptible persons into hyperendemic areas, and aging of the population contributed to the increases."}
{"id": "PMID:480581", "title": "Postoperative ulnar neuropathy.", "content": "Although compressive ulnar neuropathy developing during surgical procedures under general anesthesia has been recognized, clinical and electrophysiological features of this neuropathy have been incompletely described. During the past two years, we have seen eight patients with this complication, mainly following intra-abdominal or intrathoracic operations. Neuropathy was associated with a persistent severe deficit during a mean follow-up of 23.5 months (range, six to 96 months) after operation. Electrophysiological studies verified substantial Wallerian degeneration in the majority of patients. Clinical and electrophysiological data, as well as intraoperative findings in some patients, suggest that compression occurred at the cubital tunnel. These patients with ulnar neuropathy had a particularly poor prognosis, whether treated surgically (decompression or transposition) or medically. Prevention of ulnar nerve compression during major operations therefore assumes paramount importance.", "contents": "Postoperative ulnar neuropathy. Although compressive ulnar neuropathy developing during surgical procedures under general anesthesia has been recognized, clinical and electrophysiological features of this neuropathy have been incompletely described. During the past two years, we have seen eight patients with this complication, mainly following intra-abdominal or intrathoracic operations. Neuropathy was associated with a persistent severe deficit during a mean follow-up of 23.5 months (range, six to 96 months) after operation. Electrophysiological studies verified substantial Wallerian degeneration in the majority of patients. Clinical and electrophysiological data, as well as intraoperative findings in some patients, suggest that compression occurred at the cubital tunnel. These patients with ulnar neuropathy had a particularly poor prognosis, whether treated surgically (decompression or transposition) or medically. Prevention of ulnar nerve compression during major operations therefore assumes paramount importance."}
{"id": "PMID:480582", "title": "The need for epidemiologists.", "content": "The increasing need for epidemiologists in biomedical research has been studied by polling organizations with present and potential needs for epidemiologic services. The research institutes of the National Institutes of the National Institutes of Health and the schools of medicine reported the greatest estimated need among the respondents. They and others polled indicated a substantial need for master's and doctoral level epidemiologists that cannot be fulfilled by current training programs in epidemiology. Broad, sound methodological training applicable to all health problems, and increased funding for support of epidemiologic trainees and training programs in institutions, which would make epidemiology training competitive with that in other fields, are strongly recommened.", "contents": "The need for epidemiologists. The increasing need for epidemiologists in biomedical research has been studied by polling organizations with present and potential needs for epidemiologic services. The research institutes of the National Institutes of the National Institutes of Health and the schools of medicine reported the greatest estimated need among the respondents. They and others polled indicated a substantial need for master's and doctoral level epidemiologists that cannot be fulfilled by current training programs in epidemiology. Broad, sound methodological training applicable to all health problems, and increased funding for support of epidemiologic trainees and training programs in institutions, which would make epidemiology training competitive with that in other fields, are strongly recommened."}
{"id": "PMID:480599", "title": "Primary lymphomas of the thyroid gland.", "content": "Twelve patients with primary lymphoma of the thyroid gland were reviewed. Based on clinical observations of this group of patients and a review of the literature, a composite clinical picture can be drawn of the patient at high risk of having primary lymphoma of the thyroid gland. This condition should be considered in the differential diagnosis in patients with a history of lymphocytic thyroiditis and an enlarging neck mass, tender or nontender. The mass may be cold or cool with areas of patchy uptake shown on scintillation scanning. The patient has a greater than 50% chance of having hoarseness with or without vocal cord paralysis. We also review the pathogenesis and prognosis of primary lymphoma of the thyroid gland.", "contents": "Primary lymphomas of the thyroid gland. Twelve patients with primary lymphoma of the thyroid gland were reviewed. Based on clinical observations of this group of patients and a review of the literature, a composite clinical picture can be drawn of the patient at high risk of having primary lymphoma of the thyroid gland. This condition should be considered in the differential diagnosis in patients with a history of lymphocytic thyroiditis and an enlarging neck mass, tender or nontender. The mass may be cold or cool with areas of patchy uptake shown on scintillation scanning. The patient has a greater than 50% chance of having hoarseness with or without vocal cord paralysis. We also review the pathogenesis and prognosis of primary lymphoma of the thyroid gland."}
{"id": "PMID:480600", "title": "Bone metastases and bone pain in breast cancer. Are they closely associated?", "content": "One hundred ninety patients with breast cancer were prospectively evaluated for bone pain and had technetium Tc 99m-methylene diphosphonate bone scintigraphy for bone metastases. Of the 66 patients showing evidence for bone metastases, 21 (32%) did not have bone pain. There were 155 sites of skeletal metastases, but pain was found only in 50 sites. The age of the patient or involvement of weight-bearing bones did not seem to affect the association between bone metastases and pain. We discuss the need for periodic bone scintigraphy, even when the clinical state does not seem to warrant it.", "contents": "Bone metastases and bone pain in breast cancer. Are they closely associated? One hundred ninety patients with breast cancer were prospectively evaluated for bone pain and had technetium Tc 99m-methylene diphosphonate bone scintigraphy for bone metastases. Of the 66 patients showing evidence for bone metastases, 21 (32%) did not have bone pain. There were 155 sites of skeletal metastases, but pain was found only in 50 sites. The age of the patient or involvement of weight-bearing bones did not seem to affect the association between bone metastases and pain. We discuss the need for periodic bone scintigraphy, even when the clinical state does not seem to warrant it."}
{"id": "PMID:480601", "title": "Hemagglutination-inhibiting antibodies in vaccinated children with renal disease.", "content": "One year after immunization with a single 0.5-mL dose of influenza virus vaccine, serum samples from 30 children with renal diseases were tested for serum hemagglutination-inhibiting (HI) antibody titers to A/New Jersey/76, A/Victoria/75, and A/USSR/77. Eleven  unvaccinated children with renal diseases formed a comparison group. In contrast to the comparison group (0/11), 53% (16/30) of the vaccinated group had a protective level of serum HI titers (greater than or equal 1:40) against A/New Jersey. A protective level of serum HI titer against A/Victoria was noted in 83% (25/30) of the vaccinated group, while 54% (6/11) of the unvaccinated group had similar HI titers. None had a detectable HI titer against A/USSR. A minor common cold-like illness occurred in seven of the 30 vaccinated children; one of these had exacerbation of nephrotic syndrome. The data suggest a good protection against influenza one year after vaccination in children with renal disease.", "contents": "Hemagglutination-inhibiting antibodies in vaccinated children with renal disease. One year after immunization with a single 0.5-mL dose of influenza virus vaccine, serum samples from 30 children with renal diseases were tested for serum hemagglutination-inhibiting (HI) antibody titers to A/New Jersey/76, A/Victoria/75, and A/USSR/77. Eleven  unvaccinated children with renal diseases formed a comparison group. In contrast to the comparison group (0/11), 53% (16/30) of the vaccinated group had a protective level of serum HI titers (greater than or equal 1:40) against A/New Jersey. A protective level of serum HI titer against A/Victoria was noted in 83% (25/30) of the vaccinated group, while 54% (6/11) of the unvaccinated group had similar HI titers. None had a detectable HI titer against A/USSR. A minor common cold-like illness occurred in seven of the 30 vaccinated children; one of these had exacerbation of nephrotic syndrome. The data suggest a good protection against influenza one year after vaccination in children with renal disease."}
{"id": "PMID:480604", "title": "Nonfluorescent tinea capitis in Charleston, SC. A diagnostic problem.", "content": "Statistics gathered at the Medical University of South Carolina from 1973 to 1978 indicate a dramatic change in the etiologic agents of tinea capitis in Charleston since the 1950s. The preponderant agent is now Trichophyton tonsurans, which produces lesions that are not fluorescent in a Wood's lamp examination (long-wave ultraviolet). Trichophyton tonsurans caused 90.6% of 265 culture-proved cases of tinea capitis in the 1970s, but in the 1950s it was responsible for only 1.6% of 378 cases. The study emphasizes that cultures are necessary because the increase in nonfluorescent tinea capitis throughout the United States presents unsuspected diagnostic problems to physicians who are not aware of the change in etiologic agents.", "contents": "Nonfluorescent tinea capitis in Charleston, SC. A diagnostic problem. Statistics gathered at the Medical University of South Carolina from 1973 to 1978 indicate a dramatic change in the etiologic agents of tinea capitis in Charleston since the 1950s. The preponderant agent is now Trichophyton tonsurans, which produces lesions that are not fluorescent in a Wood's lamp examination (long-wave ultraviolet). Trichophyton tonsurans caused 90.6% of 265 culture-proved cases of tinea capitis in the 1970s, but in the 1950s it was responsible for only 1.6% of 378 cases. The study emphasizes that cultures are necessary because the increase in nonfluorescent tinea capitis throughout the United States presents unsuspected diagnostic problems to physicians who are not aware of the change in etiologic agents."}
{"id": "PMID:480620", "title": "Cytotoxic-induced ovarian failure in women with Hodgkin's disease. I. Hormone function.", "content": "Forty-one women with advanced Hodgkin's disease were studied to determine the effect of combination chemotherapy on fertility and ovarian function. Histories and pretreatment ovarian biopsy specimens indicated normal fertility before therapy, thus implying no adverse effect of Hodgkin's diseases on female gonadal function. Ovarian activity was assessed after therapy by menstrual history, serial basal body temperatures, and hormonal levels. Each case was assigned to one of three categories: primary ovarian failure (failed ovary), irregular ovarian activity (failing ovary), and normal cyclic ovarian activity (functioning ovary). After therapy, 20 of 41 patients (49%) were categorized as failed, 14 (34%) as failing, and only 7 (17%) as functioning. In 16 months of further observation, progressive loss of ovarian function occurred that was clearly age related but not statistically dose related. Induction of premature, irreversible menopause presents a need for effective hormonal replacement and patient counseling.", "contents": "Cytotoxic-induced ovarian failure in women with Hodgkin's disease. I. Hormone function. Forty-one women with advanced Hodgkin's disease were studied to determine the effect of combination chemotherapy on fertility and ovarian function. Histories and pretreatment ovarian biopsy specimens indicated normal fertility before therapy, thus implying no adverse effect of Hodgkin's diseases on female gonadal function. Ovarian activity was assessed after therapy by menstrual history, serial basal body temperatures, and hormonal levels. Each case was assigned to one of three categories: primary ovarian failure (failed ovary), irregular ovarian activity (failing ovary), and normal cyclic ovarian activity (functioning ovary). After therapy, 20 of 41 patients (49%) were categorized as failed, 14 (34%) as failing, and only 7 (17%) as functioning. In 16 months of further observation, progressive loss of ovarian function occurred that was clearly age related but not statistically dose related. Induction of premature, irreversible menopause presents a need for effective hormonal replacement and patient counseling."}
{"id": "PMID:480621", "title": "Cytotoxic-induced ovarian failure in Hodgkin's disease. II. Effects on sexual function.", "content": "Chemotheraphy for Hodgkin's disease produces ovarian failure in young women. The consequences of this are emotional distress, sexual dysfunction, and the disruption of families and friendships. These previously unappreciated side effects of cytotoxic therapy had developed in 25 of 41 patients, among whom we conducted a retrospective study.", "contents": "Cytotoxic-induced ovarian failure in Hodgkin's disease. II. Effects on sexual function. Chemotheraphy for Hodgkin's disease produces ovarian failure in young women. The consequences of this are emotional distress, sexual dysfunction, and the disruption of families and friendships. These previously unappreciated side effects of cytotoxic therapy had developed in 25 of 41 patients, among whom we conducted a retrospective study."}
{"id": "PMID:480622", "title": "Sulindac in ankylosing spondylitis. Double-blind evaluation of sulindac and indomethacin.", "content": "Ankylosing spondylitis affects about 1% of the population. In the past, evaluation of therapy in the management of this disease has been hampered by the lack of availability of objective criteria for following the condition. By using recently developed measurements of spinal mobility and other variables we have compared sulindac, a recently introduced nonsteroidal antiinflammatory drug, and indomethacin in a double-blind six-month parallel study of 30 patients. Sulindac and indomethacin have comparable efficacy and tolerance. Advantages of sulindac include a twice-a-day dose regimen.", "contents": "Sulindac in ankylosing spondylitis. Double-blind evaluation of sulindac and indomethacin. Ankylosing spondylitis affects about 1% of the population. In the past, evaluation of therapy in the management of this disease has been hampered by the lack of availability of objective criteria for following the condition. By using recently developed measurements of spinal mobility and other variables we have compared sulindac, a recently introduced nonsteroidal antiinflammatory drug, and indomethacin in a double-blind six-month parallel study of 30 patients. Sulindac and indomethacin have comparable efficacy and tolerance. Advantages of sulindac include a twice-a-day dose regimen."}
{"id": "PMID:480623", "title": "Are microcomputers appropriate for your practice?", "content": "The overly simplistic view of the process of automation widely presented to physicians needs to be counterbalanced. Computers are becoming cheaper, easier to program, and simpler to operate. However, the physician must be aware of the gap between personal computing and serious use of computers in his practice. To bridge this gap, the physician must analyze his needs to identify appropriate applications to automate, plan the implementation of these applications, and choose the necessary hardware and software resources. To do so it may be valuable for him to seek the help of an expert. Although the physician can certainly perform the necessary tasks, he should be aware that to perform them efficiently there are a number of aspects he should seriously consider.", "contents": "Are microcomputers appropriate for your practice? The overly simplistic view of the process of automation widely presented to physicians needs to be counterbalanced. Computers are becoming cheaper, easier to program, and simpler to operate. However, the physician must be aware of the gap between personal computing and serious use of computers in his practice. To bridge this gap, the physician must analyze his needs to identify appropriate applications to automate, plan the implementation of these applications, and choose the necessary hardware and software resources. To do so it may be valuable for him to seek the help of an expert. Although the physician can certainly perform the necessary tasks, he should be aware that to perform them efficiently there are a number of aspects he should seriously consider."}
{"id": "PMID:480625", "title": "Esophageal motor dysfunction in systemic lupus erythematosus. Two cases with unusual features.", "content": "Two patients with systemic lupus erythematosus had intermittent episodes of dysphagia associated with severe nonpleuritic chest pain. Esophageal manometry disclosed abnormalities characteristic of diffuse esophageal spasm. The findings suggest that diffuse spasm should be considered in the differential diagnosis of unexplained chest pain and dysphagia in patients with lupus.", "contents": "Esophageal motor dysfunction in systemic lupus erythematosus. Two cases with unusual features. Two patients with systemic lupus erythematosus had intermittent episodes of dysphagia associated with severe nonpleuritic chest pain. Esophageal manometry disclosed abnormalities characteristic of diffuse esophageal spasm. The findings suggest that diffuse spasm should be considered in the differential diagnosis of unexplained chest pain and dysphagia in patients with lupus."}
{"id": "PMID:480626", "title": "Cytotoxic chemotherapy and gonadal function in patients with Hodgkin's disease. Facts and thoughts.", "content": "Castration commonly appears to be the price of \"cure\" in patients treated with chemotheraphy for Hodgkin's disease. Awareness and anticipation by the physician, with appropriate counseling of the patient on the profound psychological and emotional changes occasioned by chemotherapeutic treatment, may provide benefit both in terms of acceptance of therapy and in adjustment of changes in interpersonal relationships. From this point, attention should be directed to (1) replacement of evident hormonal deficiency, (2) artificial preservation of viable germ cells, and (3) means of avoidance of germ cell damage by chemotherapy.", "contents": "Cytotoxic chemotherapy and gonadal function in patients with Hodgkin's disease. Facts and thoughts. Castration commonly appears to be the price of \"cure\" in patients treated with chemotheraphy for Hodgkin's disease. Awareness and anticipation by the physician, with appropriate counseling of the patient on the profound psychological and emotional changes occasioned by chemotherapeutic treatment, may provide benefit both in terms of acceptance of therapy and in adjustment of changes in interpersonal relationships. From this point, attention should be directed to (1) replacement of evident hormonal deficiency, (2) artificial preservation of viable germ cells, and (3) means of avoidance of germ cell damage by chemotherapy."}
{"id": "PMID:480642", "title": "Prophylaxis of recurrent urinary tract infection in female patients. Efficacy of low-dose, thrice-weekly therapy with trimethoprim-sulfamethoxazole.", "content": "Thirty-two women with recurrent urinary tract infections were treated after eradication of existing infections with a mixture of 40 mg of trimethoprim and 200 mg of sulfamethoxazole thrice weekly at bedtime for six months. Six preadolescents received one half this dose. During 21.3 cumulative patient-years of prophylaxis, one infection due to Streptococcus faecalis and one due to a sulfamthoxazole-and trimethoprim-sensitive Escherichia coli occurred--an infection incidence of 0.1 per patient-year. During prophylaxis, 61 of 72 periurethral cultures and 24 of 51 anal canal cultures failed to yield Enterobacteriaceae. One patient had transient colonization with a trimethoprim-resistant E coli during prophylaxis. Twenty-one patients had recurrent infection within six months of discontinuation of prophylaxis, with a mean time to recurrence of 2.6 months. One infection recurred 26 weeks following prophylaxis with a Proteus mirabilis. Thrice-weekly trimethoprim-sulfamethoxazole therapy was effective for prophylaxis of recurrent urinary tract infections and did not predispose to colonization or infection with trimethoprim-resistant Enterobacteriaceae.", "contents": "Prophylaxis of recurrent urinary tract infection in female patients. Efficacy of low-dose, thrice-weekly therapy with trimethoprim-sulfamethoxazole. Thirty-two women with recurrent urinary tract infections were treated after eradication of existing infections with a mixture of 40 mg of trimethoprim and 200 mg of sulfamethoxazole thrice weekly at bedtime for six months. Six preadolescents received one half this dose. During 21.3 cumulative patient-years of prophylaxis, one infection due to Streptococcus faecalis and one due to a sulfamthoxazole-and trimethoprim-sensitive Escherichia coli occurred--an infection incidence of 0.1 per patient-year. During prophylaxis, 61 of 72 periurethral cultures and 24 of 51 anal canal cultures failed to yield Enterobacteriaceae. One patient had transient colonization with a trimethoprim-resistant E coli during prophylaxis. Twenty-one patients had recurrent infection within six months of discontinuation of prophylaxis, with a mean time to recurrence of 2.6 months. One infection recurred 26 weeks following prophylaxis with a Proteus mirabilis. Thrice-weekly trimethoprim-sulfamethoxazole therapy was effective for prophylaxis of recurrent urinary tract infections and did not predispose to colonization or infection with trimethoprim-resistant Enterobacteriaceae."}
{"id": "PMID:480643", "title": "Yersinia enterocolitica enteritis in children.", "content": "Five patients were admitted to Children's Hospital National Medical Center with manifestations of Yersinia enterocolitica infections. Four of five had primarily enteric illness, while the fifth had associated ocular and joint involvements. In three of five cases, the disease was self-limiting. Two of the patients (No. 1 and 2) may have had their disease improved by antibiotic therapy. Increased alertness to the potential of Y enterocolitica to cause a variety of syndromes is necessary to make an accurate bacteriologic diagnosis and to expedite medical treatment.", "contents": "Yersinia enterocolitica enteritis in children. Five patients were admitted to Children's Hospital National Medical Center with manifestations of Yersinia enterocolitica infections. Four of five had primarily enteric illness, while the fifth had associated ocular and joint involvements. In three of five cases, the disease was self-limiting. Two of the patients (No. 1 and 2) may have had their disease improved by antibiotic therapy. Increased alertness to the potential of Y enterocolitica to cause a variety of syndromes is necessary to make an accurate bacteriologic diagnosis and to expedite medical treatment."}
{"id": "PMID:480644", "title": "Antibiotic usage in a pediatric medical center.", "content": "Antibiotic usage in a large pediatric teaching hospital was evaluated during a three-month period. A total of 1,067 patients received antibiotics and 295 patient records were reviewed. One hundred sixty-nine courses of antibiotics were administered to surgical patients, 77% for surgical prophylaxis. One hundred forty-seven antibiotic courses administered to medical patients were therapeutic in 61.2% and in 35.3% were given because of suspicion of infection. Ampicillin was the most commonly used antibiotic; cephalosporins were used for surgical prophylaxis. Aminoglycosides were used commonly for suspected infection in medical patients. Antibiotic management was inappropriate in 66% of surgical patients and in 21% of medical patients. Not obtaining appropriate studies prior to and during therapy were common infractions on all services. Surgical prophylaxis was freqently inappropriate by reason of no indication, wrong drug or dose, or inappropriate timing.", "contents": "Antibiotic usage in a pediatric medical center. Antibiotic usage in a large pediatric teaching hospital was evaluated during a three-month period. A total of 1,067 patients received antibiotics and 295 patient records were reviewed. One hundred sixty-nine courses of antibiotics were administered to surgical patients, 77% for surgical prophylaxis. One hundred forty-seven antibiotic courses administered to medical patients were therapeutic in 61.2% and in 35.3% were given because of suspicion of infection. Ampicillin was the most commonly used antibiotic; cephalosporins were used for surgical prophylaxis. Aminoglycosides were used commonly for suspected infection in medical patients. Antibiotic management was inappropriate in 66% of surgical patients and in 21% of medical patients. Not obtaining appropriate studies prior to and during therapy were common infractions on all services. Surgical prophylaxis was freqently inappropriate by reason of no indication, wrong drug or dose, or inappropriate timing."}
{"id": "PMID:480645", "title": "Brain death--an opposing viewpoint.", "content": "Recent and proposed legislation to establish \"brain-related\" criteria of death has uniformly confounded irreversible cessation of total brain function with the death of the human person. Much of the confusion comes from widespread misunderstanding of how the word \"death\" is used and what it means. Cessation of total brain function, whether irreversible or not, is not necessarily linked to total destruction of the brain or to the death of the person. Further, to take vital organs or to otherwise treat people as though they were dead already on the basis of these recent criteria is morally unacceptable to most Orthodox Jews and Christians.", "contents": "Brain death--an opposing viewpoint. Recent and proposed legislation to establish \"brain-related\" criteria of death has uniformly confounded irreversible cessation of total brain function with the death of the human person. Much of the confusion comes from widespread misunderstanding of how the word \"death\" is used and what it means. Cessation of total brain function, whether irreversible or not, is not necessarily linked to total destruction of the brain or to the death of the person. Further, to take vital organs or to otherwise treat people as though they were dead already on the basis of these recent criteria is morally unacceptable to most Orthodox Jews and Christians."}
{"id": "PMID:480646", "title": "An algorithm for the operational assessment of adverse drug reactions. III. Results of tests among clinicians.", "content": "To determine how practicing clinicians use a recently developed algorithm for the diagnostic assessment of suspected adverse drug reactions (ADRs), eight clinicians--four board-certified, practicing physicians and four interns--rated the likelihood of 30 suspected ADRs. Each physician reviewed the case summaries, first using implicit clinical judgment and two months later by means of the ADR algorithm. The algorithm significantly improved the reproducibility of the senior clinicians' assessments as compared with their implicit assessments; however, the improvement in the interns' assessments with the algorithm was not significant. The validity of the physicians' assessments, which was measured by comparing their ratings with a consensus rating of the three algorithm developers, was also significantly improved by the use of the algorithm. When used by practicing clinicians, the algorithm improves the reproducibility and validity of their assessments of ADRs and should provide a more precise diagnostic approach to these complex clinical phenomena.", "contents": "An algorithm for the operational assessment of adverse drug reactions. III. Results of tests among clinicians. To determine how practicing clinicians use a recently developed algorithm for the diagnostic assessment of suspected adverse drug reactions (ADRs), eight clinicians--four board-certified, practicing physicians and four interns--rated the likelihood of 30 suspected ADRs. Each physician reviewed the case summaries, first using implicit clinical judgment and two months later by means of the ADR algorithm. The algorithm significantly improved the reproducibility of the senior clinicians' assessments as compared with their implicit assessments; however, the improvement in the interns' assessments with the algorithm was not significant. The validity of the physicians' assessments, which was measured by comparing their ratings with a consensus rating of the three algorithm developers, was also significantly improved by the use of the algorithm. When used by practicing clinicians, the algorithm improves the reproducibility and validity of their assessments of ADRs and should provide a more precise diagnostic approach to these complex clinical phenomena."}
{"id": "PMID:480647", "title": "Evaluation of carcinoma of the uterine cervix before radiotherapy.", "content": "In 1974 the Patterns of Care Study began a prospective nationwide survey of radiotherapeutic cancer care in the United States. Cancer of the uterine cervix was one of the diseases surveyed. Pretreatment evaluation data were collected from 170 randomly chosen radiation therapy facilities. The frequency of use of tests in workup varied substantially according, for example, to the severity of disease and the availability of tests. For carcinoma of the uterine cervix, we found that the following tests were used routinely: physical examination, including taking a history; complete blood cell count; serum chemistry analysis; chest roentgenogram; and intravenous pyelogram. Frequent but not routine tests included dilation and curettage and evaluation of the involvement of the bladder and rectum. Other test were used infrequently.", "contents": "Evaluation of carcinoma of the uterine cervix before radiotherapy. In 1974 the Patterns of Care Study began a prospective nationwide survey of radiotherapeutic cancer care in the United States. Cancer of the uterine cervix was one of the diseases surveyed. Pretreatment evaluation data were collected from 170 randomly chosen radiation therapy facilities. The frequency of use of tests in workup varied substantially according, for example, to the severity of disease and the availability of tests. For carcinoma of the uterine cervix, we found that the following tests were used routinely: physical examination, including taking a history; complete blood cell count; serum chemistry analysis; chest roentgenogram; and intravenous pyelogram. Frequent but not routine tests included dilation and curettage and evaluation of the involvement of the bladder and rectum. Other test were used infrequently."}
{"id": "PMID:480648", "title": "Rickettsialpox. Case report and epidemiologic review.", "content": "In recent years, rickettsialpox was infrequently reported: one case per year was recorded by the Public Health Service during the past 15 years. We observed a case of rickettsialpox in New York City and reviewed the clinical, diagnostic, and epidemiologic features. Records of the New York City Health Department showed that serum samples from only six patients were submitted for serological testing for rickettsialpox during the past ten years, suggesting that although the disease is uncommon now, clinicians may be failing to recognize and diagnose rickettsialpox, as it may occur more frequently than is presently recognized.", "contents": "Rickettsialpox. Case report and epidemiologic review. In recent years, rickettsialpox was infrequently reported: one case per year was recorded by the Public Health Service during the past 15 years. We observed a case of rickettsialpox in New York City and reviewed the clinical, diagnostic, and epidemiologic features. Records of the New York City Health Department showed that serum samples from only six patients were submitted for serological testing for rickettsialpox during the past ten years, suggesting that although the disease is uncommon now, clinicians may be failing to recognize and diagnose rickettsialpox, as it may occur more frequently than is presently recognized."}
{"id": "PMID:480675", "title": "Lymphocyte transformation in patients with breast cancer and the effect of surgery.", "content": "Lymphocyte response to phytohemagglutinin (PHA) in vitro was subnormal in forty of fifty-one patients with breast cancer. After apparently successful surgery, lymphocyte response improved significantly in most of those in whom it had been previously impaired. It remained markedly subnormal in only five patients, two of whome subsequently died of metastases. This finding indicates that depressed cell mediated immunity in patients with breast cancer is the result of the malignant disease. Failure of the PHA response to revert to normal postoperatively may indicate a poor prognosis.", "contents": "Lymphocyte transformation in patients with breast cancer and the effect of surgery. Lymphocyte response to phytohemagglutinin (PHA) in vitro was subnormal in forty of fifty-one patients with breast cancer. After apparently successful surgery, lymphocyte response improved significantly in most of those in whom it had been previously impaired. It remained markedly subnormal in only five patients, two of whome subsequently died of metastases. This finding indicates that depressed cell mediated immunity in patients with breast cancer is the result of the malignant disease. Failure of the PHA response to revert to normal postoperatively may indicate a poor prognosis."}
{"id": "PMID:480676", "title": "Longitudinal study on Schistosoma japonicum infections in the Philippines. Incidence and prevalence among school children in Dagami Area, Leyte.", "content": "The annual incidence of Schistosoma japonicum infection was calculated from the record of the yearly examination among school children of Dagami Area, Leyte, Philippines. Data were processed mainly by the computer. Children were examined for eggs by the merthiolate formalin concentration technique and by the circumoval precipitin test (COPT) once a year in 3 consecutive school years (SY), SY 1974/75, 1975/76 and 1976/77. About 600 to 700 children were examined yearly and the ratios of cases rechecked after 1 year were about 30%. A method to calculate the overall incidence in children who were examined at different intervals, was newly established. The incidence appeared to rise rapidly in the survey period, being 25.59% and 41.30% by fecal examination and 10.74% and 21.27% by COPT in SY 1975/76 and 1976/77, respectively. From the combined results of fecal examination and COPT, the reliable values of incidence, however, were shown to be stable during 2 years such as 22.06% and 24.21%, respectively. The incidence estimated by the age prevalence data was 20.25% in SY 1976/77 by the combined results and was found to be lower than the directly calculated value.", "contents": "Longitudinal study on Schistosoma japonicum infections in the Philippines. Incidence and prevalence among school children in Dagami Area, Leyte. The annual incidence of Schistosoma japonicum infection was calculated from the record of the yearly examination among school children of Dagami Area, Leyte, Philippines. Data were processed mainly by the computer. Children were examined for eggs by the merthiolate formalin concentration technique and by the circumoval precipitin test (COPT) once a year in 3 consecutive school years (SY), SY 1974/75, 1975/76 and 1976/77. About 600 to 700 children were examined yearly and the ratios of cases rechecked after 1 year were about 30%. A method to calculate the overall incidence in children who were examined at different intervals, was newly established. The incidence appeared to rise rapidly in the survey period, being 25.59% and 41.30% by fecal examination and 10.74% and 21.27% by COPT in SY 1975/76 and 1976/77, respectively. From the combined results of fecal examination and COPT, the reliable values of incidence, however, were shown to be stable during 2 years such as 22.06% and 24.21%, respectively. The incidence estimated by the age prevalence data was 20.25% in SY 1976/77 by the combined results and was found to be lower than the directly calculated value."}
{"id": "PMID:480680", "title": "Urinary 3-methoxy-4-hydroxyphenylglycol (MHPG) excretion in depression.", "content": "Twelve acute depressed patients were studied in the course of antidepressant treatment. Three-day-24-hour urine samples for 3-methoxy-4-hydroxy-phenylglycol (MHPG) were collected both before and after a five week treatment schedule. MHPG were estimated by spectrophotometry. Initial MHPG excretion did not reflect the severity of pretreatment depression and final MHPG showed no specific correlation with post-treatment improvement. No specific or uniform influence of different anti-depressant treatments or changes in MHPG was evident. Depressed patients with bipolar affective disorder tend to have initially lower MHPG levels than patients with unipolar depression. Urinary MHPG excretion measurements may prove not to be a useful index in the diagnosis and choice of treatment in patients suffering from primary depression.", "contents": "Urinary 3-methoxy-4-hydroxyphenylglycol (MHPG) excretion in depression. Twelve acute depressed patients were studied in the course of antidepressant treatment. Three-day-24-hour urine samples for 3-methoxy-4-hydroxy-phenylglycol (MHPG) were collected both before and after a five week treatment schedule. MHPG were estimated by spectrophotometry. Initial MHPG excretion did not reflect the severity of pretreatment depression and final MHPG showed no specific correlation with post-treatment improvement. No specific or uniform influence of different anti-depressant treatments or changes in MHPG was evident. Depressed patients with bipolar affective disorder tend to have initially lower MHPG levels than patients with unipolar depression. Urinary MHPG excretion measurements may prove not to be a useful index in the diagnosis and choice of treatment in patients suffering from primary depression."}
{"id": "PMID:480781", "title": "Effects of 24-hour unilateral ureteral obstruction on glomerular hemodynamics in rat kidney.", "content": "Glomerular hemodynamics were studied, by micropuncture, in Munich-Wistar rats submitted to 24-hour unilateral ureteral ligation (UUL). Glomerular capillary pressure (PG), intratubular pressure (PT) and pressure in the first-order peritubular capillaries (EAP) were measured with a servonulling device. Single nephron filtration fraction (SNIFF) was calculated fmom arterial and peritubular blood protein concentration. SNGFR was both measured by conventional micropuncture techniques and calculated from efferent arteriole blood flow (EABF) and SNFF. Afferent arteriole blood flow (AABF) and resistance of afferent (Ra) and efferent (Re) arterioles were calculated. Measurements were repeated 1 to 2 hours after the release of the ureter. Sham-operated rats were used as control. UUL caused a marked increase in Ra (from 4.9 +/- [SD] 2.4 to 12.7 +/- 5.1 dynes/sec/cm-5). The fall in SNGFR (from 111.9 +/- [SD] 23.9 to 34.4 +/- 23.1 nl/min/kg body wt) was secondary to a decrease in both PG and AABF. A further increase in Ra (16.0 +/- 6.7 dynes.sec.cm-5) occurred after releasing the ureter. SNGFR, however, was unaltered (33.7 +/- 16.6 nl/min/kg body wt) since PG decreased parallel to PT, but AABF did not significantly change. Conclusion. Ureteral obstruction determines, in 24 hours, a marked cortical ischemia that is not promptly reversed by ureteral release.", "contents": "Effects of 24-hour unilateral ureteral obstruction on glomerular hemodynamics in rat kidney. Glomerular hemodynamics were studied, by micropuncture, in Munich-Wistar rats submitted to 24-hour unilateral ureteral ligation (UUL). Glomerular capillary pressure (PG), intratubular pressure (PT) and pressure in the first-order peritubular capillaries (EAP) were measured with a servonulling device. Single nephron filtration fraction (SNIFF) was calculated fmom arterial and peritubular blood protein concentration. SNGFR was both measured by conventional micropuncture techniques and calculated from efferent arteriole blood flow (EABF) and SNFF. Afferent arteriole blood flow (AABF) and resistance of afferent (Ra) and efferent (Re) arterioles were calculated. Measurements were repeated 1 to 2 hours after the release of the ureter. Sham-operated rats were used as control. UUL caused a marked increase in Ra (from 4.9 +/- [SD] 2.4 to 12.7 +/- 5.1 dynes/sec/cm-5). The fall in SNGFR (from 111.9 +/- [SD] 23.9 to 34.4 +/- 23.1 nl/min/kg body wt) was secondary to a decrease in both PG and AABF. A further increase in Ra (16.0 +/- 6.7 dynes.sec.cm-5) occurred after releasing the ureter. SNGFR, however, was unaltered (33.7 +/- 16.6 nl/min/kg body wt) since PG decreased parallel to PT, but AABF did not significantly change. Conclusion. Ureteral obstruction determines, in 24 hours, a marked cortical ischemia that is not promptly reversed by ureteral release."}
{"id": "PMID:480782", "title": "Medullary collecting duct function in the remnant kidney before and after volume expansion.", "content": "The role of the medullary collecting ducts (CD) in the regulation of water and electrolyte excretion by the remnant kidney has not been determined. Medullary CD function was therefore studied by the microcatheterization technique during hydropenia and after volume expansion with an isotonic saline load in rats with sham-operated normal kidney (stage I), remnant kidney (stage II), remnant kidney after contralateral nephrectomy (stage III), and sham-operated normal kidney after contralateral nephrectomy (stage-III control). Sodium, potassium, water, and solute reabsorption along the medullary CD were not altered in stage II during hydropenia when compared to normal control (stage I), but water reabsorption proximal to the medullary CD was decreased. In stage III, where the uremia was mild (BUN, 41 mg/dl), the fractions of the filtered load of sodium (72%) and water (62%) which were reabsorbed along the medullary CD were reduced in comparison to stage II (94% and 83%, respectively), stage I, or stage-III control. The fraction of filtered potassium entering the medullary CD was increased to 53% in stage III, compared to 16% in stage II, and 10 or 11% in stage I or stage-III control. No change in the fraction of filtered potassium remaining along the collecting ducts was observed. After volume expansion, there was no significant change in the fraction of filtered sodium and water remaining along the medullary CD in stage I, II, or III. The greater fractional excretion in stage III resulted from decreased reabsorption in more proximal nephron segments. The results indicate that (a) during hydropenia, fractional reabsorption of sodium and water is decreased along the medullary CD of the stage-III remnant kidney in the presence of mild uremia, (b) the increased fractional excretion of potassium in the remnant kidney with uremia is not determined by altered potassium handling in the medullary CD but occurs proximal to this nephron segment, and (c) extracellular fluid volume expansion with isotonic saline results in similar inhibition of fractional sodium and water reabsorption in the collecting ducts of both remnant and normal kidneys.", "contents": "Medullary collecting duct function in the remnant kidney before and after volume expansion. The role of the medullary collecting ducts (CD) in the regulation of water and electrolyte excretion by the remnant kidney has not been determined. Medullary CD function was therefore studied by the microcatheterization technique during hydropenia and after volume expansion with an isotonic saline load in rats with sham-operated normal kidney (stage I), remnant kidney (stage II), remnant kidney after contralateral nephrectomy (stage III), and sham-operated normal kidney after contralateral nephrectomy (stage-III control). Sodium, potassium, water, and solute reabsorption along the medullary CD were not altered in stage II during hydropenia when compared to normal control (stage I), but water reabsorption proximal to the medullary CD was decreased. In stage III, where the uremia was mild (BUN, 41 mg/dl), the fractions of the filtered load of sodium (72%) and water (62%) which were reabsorbed along the medullary CD were reduced in comparison to stage II (94% and 83%, respectively), stage I, or stage-III control. The fraction of filtered potassium entering the medullary CD was increased to 53% in stage III, compared to 16% in stage II, and 10 or 11% in stage I or stage-III control. No change in the fraction of filtered potassium remaining along the collecting ducts was observed. After volume expansion, there was no significant change in the fraction of filtered sodium and water remaining along the medullary CD in stage I, II, or III. The greater fractional excretion in stage III resulted from decreased reabsorption in more proximal nephron segments. The results indicate that (a) during hydropenia, fractional reabsorption of sodium and water is decreased along the medullary CD of the stage-III remnant kidney in the presence of mild uremia, (b) the increased fractional excretion of potassium in the remnant kidney with uremia is not determined by altered potassium handling in the medullary CD but occurs proximal to this nephron segment, and (c) extracellular fluid volume expansion with isotonic saline results in similar inhibition of fractional sodium and water reabsorption in the collecting ducts of both remnant and normal kidneys."}
{"id": "PMID:480783", "title": "Glomerular filtration dynamics in the dog during elevated plasma colloid osmotic pressure.", "content": "To determine if the glomerular filtration coefficient (Kf) of the dog is influenced by changes in plasma colloid osmotic pressure (COP), we conducted micropuncture experiments in dogs given concentrated albumin solutions. In one group (N = 9), filtration dynamics were evaluated following infusion of 450 to 600 ml of a 25% bovine albumin solution. To minimize the effects of acute volume expansion, we also achieved high COP levels in another group (N = 7) by albumin loading on the day prior to the experiment. In all experiments, renal arterial pressure was reduced to approximately 90 mm Hg to minimize potential errors that might lead to overestimation of single nephron filtration rate (SNGFR) and glomerular pressure (GP). In the acutely expanded dogs, COP increased to 23.0 +/- (SEM) 0.9 mm Hg, SNGFR was 59 +/- 6 nl/min, estimated GP was 61.0 +/- 2.0 mm Hg, proximal tubule pressure (PTP) was 23.0 +/- 1.6 mm Hg, and superficial filtration fraction (SFF) was 0.13 +/- 0.02. A similarly reduced whole kidney filtration fraction was also observed, due almost entirely to a marked increase in renal blood flow. When compared to noninfused control dogs (N = 13), Kf was significantly higher in the dogs with elevated COP, being 5.3 +/- 0.6 nl/min/mm Hg as compared to 3.4 +/- 0.3 nl/min/mm Hg. Average effective filtration pressure (EFP) was 12 +/- 1mm Hg, and EFP at the efferent end of the glomerular capillaries was 8.9 +/- 1.2 mm Hg. In the group infused on the prior day, COP was 20.0 +/- 0.8 mm Hg, SFF was 0.26 +/- 0.01, SNGFR was 70 +/-8 nl/min, GP was 59 +/- 2 mm Hg, and PTP was 19.0 +/- 1.5 mm Hg. Average EFP was 15 +/- 1 mm Hg, and EFP at the efferent end of the capillaries was 7.5 +/- 0.7 mm Hg. kf was 4.85 +/- 0.66 nl/min/mm Hg, a value significantly higher than that obtained in control dogs having a COP of 15.0 +/- 0.6 mm Hg. Furthermore, one group of control dogs (N = 4), expanded with an isooncotic albumin solution, did not exhibit significant changes in Kf even though the degree of plasma volume expansion was similar to the group expanded with concentrated albumin solution. These experiments are consistent with previous findings obtained in the rat that Kf is influenced by the COP, although the changes in Kf appear to be less than they are in the rat. The data indicate that even under these conditions of elevated COP, the filtration process in the dog is characterized by positive filtration pressures throughout the length of the glomerular capillaries.", "contents": "Glomerular filtration dynamics in the dog during elevated plasma colloid osmotic pressure. To determine if the glomerular filtration coefficient (Kf) of the dog is influenced by changes in plasma colloid osmotic pressure (COP), we conducted micropuncture experiments in dogs given concentrated albumin solutions. In one group (N = 9), filtration dynamics were evaluated following infusion of 450 to 600 ml of a 25% bovine albumin solution. To minimize the effects of acute volume expansion, we also achieved high COP levels in another group (N = 7) by albumin loading on the day prior to the experiment. In all experiments, renal arterial pressure was reduced to approximately 90 mm Hg to minimize potential errors that might lead to overestimation of single nephron filtration rate (SNGFR) and glomerular pressure (GP). In the acutely expanded dogs, COP increased to 23.0 +/- (SEM) 0.9 mm Hg, SNGFR was 59 +/- 6 nl/min, estimated GP was 61.0 +/- 2.0 mm Hg, proximal tubule pressure (PTP) was 23.0 +/- 1.6 mm Hg, and superficial filtration fraction (SFF) was 0.13 +/- 0.02. A similarly reduced whole kidney filtration fraction was also observed, due almost entirely to a marked increase in renal blood flow. When compared to noninfused control dogs (N = 13), Kf was significantly higher in the dogs with elevated COP, being 5.3 +/- 0.6 nl/min/mm Hg as compared to 3.4 +/- 0.3 nl/min/mm Hg. Average effective filtration pressure (EFP) was 12 +/- 1mm Hg, and EFP at the efferent end of the glomerular capillaries was 8.9 +/- 1.2 mm Hg. In the group infused on the prior day, COP was 20.0 +/- 0.8 mm Hg, SFF was 0.26 +/- 0.01, SNGFR was 70 +/-8 nl/min, GP was 59 +/- 2 mm Hg, and PTP was 19.0 +/- 1.5 mm Hg. Average EFP was 15 +/- 1 mm Hg, and EFP at the efferent end of the capillaries was 7.5 +/- 0.7 mm Hg. kf was 4.85 +/- 0.66 nl/min/mm Hg, a value significantly higher than that obtained in control dogs having a COP of 15.0 +/- 0.6 mm Hg. Furthermore, one group of control dogs (N = 4), expanded with an isooncotic albumin solution, did not exhibit significant changes in Kf even though the degree of plasma volume expansion was similar to the group expanded with concentrated albumin solution. These experiments are consistent with previous findings obtained in the rat that Kf is influenced by the COP, although the changes in Kf appear to be less than they are in the rat. The data indicate that even under these conditions of elevated COP, the filtration process in the dog is characterized by positive filtration pressures throughout the length of the glomerular capillaries."}
{"id": "PMID:480784", "title": "Effects of metabolic acidosis and alkalosis on sodium and calcium transport in the dog kidney.", "content": "Clearance and micropuncture studies have been performed in dogs to examine the effects of acute and chronic metabolic acidosis and acute alkalosis on tubular sodium and calcium transport. Acute metabolic acidosis, induced by the infusion of hydrochloric acid, decreased proximal fluid reabsorption and increased the fractional delivery of sodium and calcium to the distal tubule, but not to the final urine. In comparison with normal dogs, dogs with chronic metabolic acidosis (induced by feeding ammonium chloride) showed an increase in proximal fluid reabsorption and a dissociation of calcium from sodium reabsorption more distally, leading to an increased delivery of calcium relative to sodium at the distal tubule and in the final urine. The infusion of sodium bicarbonate to correct chronic metabolic acidosis, both in intact and thyroparathyroidectomized (TPTX) dogs, reduced proximal fluid reabsorption and caused a selective enhancement of calcium reabsorption relative to sodium in the more distal nephron, resulting in a reversal of the dissociation observed in acidosis, both at the distal tubule and in the final urine. By contrastin fusion of sodium chloride in parathyroid-intact acidotic dogs did not reduce proximal fluid reabsorption or enhance tubular calcium reabsorption. In nonacidotic dogs, both intact and TPTX, infusion of sodium bicarconate to induce acute alkalosis resulted in selhese data demonstrate the presence of a component of tubular calcium reabsorption situated beyond the proximal tubule, which is inhibited by chronic (but not acute) metabolic acidosis and enhanced by metabolic alkalosis (or bicarbonate infusion) independently of parathyroid hormone.", "contents": "Effects of metabolic acidosis and alkalosis on sodium and calcium transport in the dog kidney. Clearance and micropuncture studies have been performed in dogs to examine the effects of acute and chronic metabolic acidosis and acute alkalosis on tubular sodium and calcium transport. Acute metabolic acidosis, induced by the infusion of hydrochloric acid, decreased proximal fluid reabsorption and increased the fractional delivery of sodium and calcium to the distal tubule, but not to the final urine. In comparison with normal dogs, dogs with chronic metabolic acidosis (induced by feeding ammonium chloride) showed an increase in proximal fluid reabsorption and a dissociation of calcium from sodium reabsorption more distally, leading to an increased delivery of calcium relative to sodium at the distal tubule and in the final urine. The infusion of sodium bicarbonate to correct chronic metabolic acidosis, both in intact and thyroparathyroidectomized (TPTX) dogs, reduced proximal fluid reabsorption and caused a selective enhancement of calcium reabsorption relative to sodium in the more distal nephron, resulting in a reversal of the dissociation observed in acidosis, both at the distal tubule and in the final urine. By contrastin fusion of sodium chloride in parathyroid-intact acidotic dogs did not reduce proximal fluid reabsorption or enhance tubular calcium reabsorption. In nonacidotic dogs, both intact and TPTX, infusion of sodium bicarconate to induce acute alkalosis resulted in selhese data demonstrate the presence of a component of tubular calcium reabsorption situated beyond the proximal tubule, which is inhibited by chronic (but not acute) metabolic acidosis and enhanced by metabolic alkalosis (or bicarbonate infusion) independently of parathyroid hormone."}
{"id": "PMID:480785", "title": "Effect of various protein diets on growth, renal function, and survival of uremic rats.", "content": "The effects on growth, renal function, and survival of three isocaloric diets of various protein content (14, 27, and 37 g/100 g in diets I, II, and III, respectively) were compared in uremic rats and in controls. Diet I provided the minimal requirements in all amino acids for gorwing rats. In controls fed ad lib, weight and length gain were better with high protein diets, whereas they were inversely related to the diet protein content in uremic rats. The higher the protein intake, the higher the progressive elevation of BUN and serum creatinine and the mortality rate. Because proteins were supplied by fish flour, their increase was associated with increased mineral content, and the conclusions are restricted to the use of natural proteins: a moderately restricted protein diet securing only the minimal requirements had a beneficial effect on growth and survival of rats with reduced kidney mass. Avoiding any excess in proteins from the early stage of renal disease is suggested.", "contents": "Effect of various protein diets on growth, renal function, and survival of uremic rats. The effects on growth, renal function, and survival of three isocaloric diets of various protein content (14, 27, and 37 g/100 g in diets I, II, and III, respectively) were compared in uremic rats and in controls. Diet I provided the minimal requirements in all amino acids for gorwing rats. In controls fed ad lib, weight and length gain were better with high protein diets, whereas they were inversely related to the diet protein content in uremic rats. The higher the protein intake, the higher the progressive elevation of BUN and serum creatinine and the mortality rate. Because proteins were supplied by fish flour, their increase was associated with increased mineral content, and the conclusions are restricted to the use of natural proteins: a moderately restricted protein diet securing only the minimal requirements had a beneficial effect on growth and survival of rats with reduced kidney mass. Avoiding any excess in proteins from the early stage of renal disease is suggested."}
{"id": "PMID:480786", "title": "Amino acid-mediated stimulation of renal phospholipid biosynthesis after acute tubular necrosis.", "content": "The mechanism by which amino acid infusion stimulates membrane physpholipid biosynthesis during renal regeneration after mercuric-chloride-induced acute tubular necrosis was studied in the rat. Amino acids can act directly on regenerating renal tissue to enhance net phospholipid synthesis because preincubation of cortical slices with amino acids induced an increase in [14C]-choline incorporation into phospholipid without altering the rate of breakdown. This amino acid stimulation of phospholipid biosynthesis was studied further by measuring [14C]-choline accumulation and its sequential conversion to phosphorylcholine, cytidine diphosphocholine (CDP-choline), and phosphatidylcholine via the Kennedy pathway in regenerating renal tissue. [14C]-Choline accumulation was increased after amino acid infusion, compared to glucose infusion. There were also increments in the Vmax of the choline kinase reaction, which converts entering [14C]-choline into [14C]-phosphorylcholine, and of the cholinephosphotransferase reaction in which [14C]-CDP-choline is incorporated into [14C]-phosphatidylcholine, whereas the apparent Km of each reaction was unchanged. Thus, amino acids infused after tubular necrosis can act directly on regenerating renal cells to increase precursor availability and augment two reactions of the phospholipid biosynthetic pathway.", "contents": "Amino acid-mediated stimulation of renal phospholipid biosynthesis after acute tubular necrosis. The mechanism by which amino acid infusion stimulates membrane physpholipid biosynthesis during renal regeneration after mercuric-chloride-induced acute tubular necrosis was studied in the rat. Amino acids can act directly on regenerating renal tissue to enhance net phospholipid synthesis because preincubation of cortical slices with amino acids induced an increase in [14C]-choline incorporation into phospholipid without altering the rate of breakdown. This amino acid stimulation of phospholipid biosynthesis was studied further by measuring [14C]-choline accumulation and its sequential conversion to phosphorylcholine, cytidine diphosphocholine (CDP-choline), and phosphatidylcholine via the Kennedy pathway in regenerating renal tissue. [14C]-Choline accumulation was increased after amino acid infusion, compared to glucose infusion. There were also increments in the Vmax of the choline kinase reaction, which converts entering [14C]-choline into [14C]-phosphorylcholine, and of the cholinephosphotransferase reaction in which [14C]-CDP-choline is incorporated into [14C]-phosphatidylcholine, whereas the apparent Km of each reaction was unchanged. Thus, amino acids infused after tubular necrosis can act directly on regenerating renal cells to increase precursor availability and augment two reactions of the phospholipid biosynthetic pathway."}
{"id": "PMID:480787", "title": "Prognostic importance of vascular lesions in acute renal failure with microangiopathic hemolytic anemia (hemolytic-uremic syndrome): clinicopathologic study in 20 adults.", "content": "Renal biopsies obtained from 20 adult patients within 30 days after onset of acute renal failure with microangiopathic hemolytic anemia (\"the hemolytic-uremic syndrome\") were studied. Lesions were graded independently by two observers without knowledge of the clinical history. All patients who did not have refractory hypertension were treated with heparin. Ten of the patients died, and four developed end-stage renal failure requiring chronic dialysis. Six patients, however, had a relatively good outcome: two recovered completely and four developed mild-to-moderate chronic renal failure not requiring dialysis. The six patients with a good outcome had significantly less severe arterial intimal thickening on biopsy compared with the remaining patients with a poor outcome. The patients with a good outcome and those with a poor outcome did not differ in the severity of glomerular lesions. The clinical features did not allow a prediction of late outcome. These results suggest that early renal biopsies may be helpful in predicting prognosis in the \"hemolytic-uremic syndrome.\" This clinical syndrome may occur either in apparently healthy people, or may complicate the course of a chronic essential hypertension.", "contents": "Prognostic importance of vascular lesions in acute renal failure with microangiopathic hemolytic anemia (hemolytic-uremic syndrome): clinicopathologic study in 20 adults. Renal biopsies obtained from 20 adult patients within 30 days after onset of acute renal failure with microangiopathic hemolytic anemia (\"the hemolytic-uremic syndrome\") were studied. Lesions were graded independently by two observers without knowledge of the clinical history. All patients who did not have refractory hypertension were treated with heparin. Ten of the patients died, and four developed end-stage renal failure requiring chronic dialysis. Six patients, however, had a relatively good outcome: two recovered completely and four developed mild-to-moderate chronic renal failure not requiring dialysis. The six patients with a good outcome had significantly less severe arterial intimal thickening on biopsy compared with the remaining patients with a poor outcome. The patients with a good outcome and those with a poor outcome did not differ in the severity of glomerular lesions. The clinical features did not allow a prediction of late outcome. These results suggest that early renal biopsies may be helpful in predicting prognosis in the \"hemolytic-uremic syndrome.\" This clinical syndrome may occur either in apparently healthy people, or may complicate the course of a chronic essential hypertension."}
{"id": "PMID:480790", "title": "[The crescent sign in childhood urography].", "content": "The crescent sign is a rare roentgenological symptom in excretory urography of infantile hydronephrosis. The orign is a presence of contrast material in the collecting renal tubule of the atrophic renal parenchyma. That is displaced at the renal circumference. The disappearance of the crescent sign represents the prognostic for the reparative potence of the renal parenchyma after the therapy of the obstructive uropathy in two own case.", "contents": "[The crescent sign in childhood urography]. The crescent sign is a rare roentgenological symptom in excretory urography of infantile hydronephrosis. The orign is a presence of contrast material in the collecting renal tubule of the atrophic renal parenchyma. That is displaced at the renal circumference. The disappearance of the crescent sign represents the prognostic for the reparative potence of the renal parenchyma after the therapy of the obstructive uropathy in two own case."}
{"id": "PMID:480791", "title": "[Behavior of serum concentrations of immunoglobulins G, M and A in the 1st week of illness in children with bacterial and viral diseases].", "content": "The serum concentrations of the immune globulins in children with acute infection of the upper respiratory tract, angina lacunaris, mumps and acute infection of the urinary tract within the first week of illness were analysed. In the adequate age group the values (altogether 540) of healthy and diseased children were confronted and statistically compared. Here the following characteristic changes were shown: the acute infection of the upper respiratory tract was characterized by an increase of IgG. In mumps a decrease of IgA was stated. As to the infections of the urinary tract in the younger age group an increase of IgM existed, in the older age group a decrease of IgA. The results refer to the dynamics of the immune globulins in the initial phase of the individual diseases.", "contents": "[Behavior of serum concentrations of immunoglobulins G, M and A in the 1st week of illness in children with bacterial and viral diseases]. The serum concentrations of the immune globulins in children with acute infection of the upper respiratory tract, angina lacunaris, mumps and acute infection of the urinary tract within the first week of illness were analysed. In the adequate age group the values (altogether 540) of healthy and diseased children were confronted and statistically compared. Here the following characteristic changes were shown: the acute infection of the upper respiratory tract was characterized by an increase of IgG. In mumps a decrease of IgA was stated. As to the infections of the urinary tract in the younger age group an increase of IgM existed, in the older age group a decrease of IgA. The results refer to the dynamics of the immune globulins in the initial phase of the individual diseases."}
{"id": "PMID:480792", "title": "[Social environment of the puerpera and her attitude towards the child].", "content": "The mother's attitude towards the newborn child is influenced considerably as well by the number of children, the respective mother is responsible for, as by other factors of her social environment, by the partners attitude towards mother and child, by the efforts in respect of a conscious planning of the human reproduction. Small birth orders, favourable conditions regarding family and social environment, but nevertheless conscious family planning are in high correlation with a positive attitude of the young mother towards the newborn child.", "contents": "[Social environment of the puerpera and her attitude towards the child]. The mother's attitude towards the newborn child is influenced considerably as well by the number of children, the respective mother is responsible for, as by other factors of her social environment, by the partners attitude towards mother and child, by the efforts in respect of a conscious planning of the human reproduction. Small birth orders, favourable conditions regarding family and social environment, but nevertheless conscious family planning are in high correlation with a positive attitude of the young mother towards the newborn child."}
{"id": "PMID:480796", "title": "[Retinal detachment caused by boxing].", "content": "Five cases (six eyes) of retinal detachment due to fisticuffs are recorded; at least four eyes went blind. Similar observations have been reported by other authors. In boxing, traumatic lesions of the head are inevitable since punches upon the head are crucial in this sport. Because of the risk of severe injuries including blindness and even death, at least brutal boxing must be rejected.", "contents": "[Retinal detachment caused by boxing]. Five cases (six eyes) of retinal detachment due to fisticuffs are recorded; at least four eyes went blind. Similar observations have been reported by other authors. In boxing, traumatic lesions of the head are inevitable since punches upon the head are crucial in this sport. Because of the risk of severe injuries including blindness and even death, at least brutal boxing must be rejected."}
{"id": "PMID:480797", "title": "[Length of hospital stay and immobilization of patients with retinal detachment].", "content": "Referring to the modern operation techniques (buckling procedures) a reduction of bed rest for patients with retinal detachment is recommended.", "contents": "[Length of hospital stay and immobilization of patients with retinal detachment]. Referring to the modern operation techniques (buckling procedures) a reduction of bed rest for patients with retinal detachment is recommended."}
{"id": "PMID:480798", "title": "[Refractive changes following episcleral implants and cerclage].", "content": "Following the operation about 75% of the treated eyes developed myopisation between 0,5 and 4,0 dptrs. During the first postoperative year this myopia initially decreased but beyond the sixth month after surgery no further changes could be observed.", "contents": "[Refractive changes following episcleral implants and cerclage]. Following the operation about 75% of the treated eyes developed myopisation between 0,5 and 4,0 dptrs. During the first postoperative year this myopia initially decreased but beyond the sixth month after surgery no further changes could be observed."}
{"id": "PMID:480800", "title": "[The usefulness of echography in retinal surgery (author's transl)].", "content": "Upon utilization of echography one certainly will not be able to detect retinal defects; however, other parameters of retinal detachment which by means of solely optical examination techniques may remain undiagnosed can be evaluated successfully. This concerns not only the diagnosis of retinal detachment alone but also the measurement of the height of the detachment including its possible variations, the accuracy of this method being up to 0.1 mm. This will also apply to the distinction of serous, haemorrhagic and solid detachment of the choroid, the amount of liquefaction of a subchorioidal haemorrhage as well as the assessment of the relationships between vitreous membranes and retinal detachment: this applies especially to cases with hazy media and decreased visibility of the fundus. Accordingly clinical echography may be successfully applied, prior to retinal surgery, during the operation and during the postoperative follow-up.", "contents": "[The usefulness of echography in retinal surgery (author's transl)]. Upon utilization of echography one certainly will not be able to detect retinal defects; however, other parameters of retinal detachment which by means of solely optical examination techniques may remain undiagnosed can be evaluated successfully. This concerns not only the diagnosis of retinal detachment alone but also the measurement of the height of the detachment including its possible variations, the accuracy of this method being up to 0.1 mm. This will also apply to the distinction of serous, haemorrhagic and solid detachment of the choroid, the amount of liquefaction of a subchorioidal haemorrhage as well as the assessment of the relationships between vitreous membranes and retinal detachment: this applies especially to cases with hazy media and decreased visibility of the fundus. Accordingly clinical echography may be successfully applied, prior to retinal surgery, during the operation and during the postoperative follow-up."}
{"id": "PMID:480802", "title": "[Occurrence of congenital ocular toxoplasmosis in siblings].", "content": "The author points out in connection with the description of two families in which evidently several children suffered damage through toxoplasmosis with which the mother had been infected a long time ago (pseudocoloboma of the macula, changes of the macula similar to juvenile degreneration, abortion and malformations such as anophthalmia, rhinoschisis, cheiloschisis, gnathoschisis and palatoschisis), that low titer levels in the Sabin-Feldmann test and in the complement fixation test are by no means totally insignificant for subsequent pregnancies, as is commonly assumed. In any case, women who have already given birth to children with congenital toxoplasmosis should be subjected to toxoplasmosis treatment, as far as possible, before any new pregnancy.", "contents": "[Occurrence of congenital ocular toxoplasmosis in siblings]. The author points out in connection with the description of two families in which evidently several children suffered damage through toxoplasmosis with which the mother had been infected a long time ago (pseudocoloboma of the macula, changes of the macula similar to juvenile degreneration, abortion and malformations such as anophthalmia, rhinoschisis, cheiloschisis, gnathoschisis and palatoschisis), that low titer levels in the Sabin-Feldmann test and in the complement fixation test are by no means totally insignificant for subsequent pregnancies, as is commonly assumed. In any case, women who have already given birth to children with congenital toxoplasmosis should be subjected to toxoplasmosis treatment, as far as possible, before any new pregnancy."}
{"id": "PMID:480803", "title": "[Discrete inflammatory manifestations of the posterior eye segment (author's transl)].", "content": "White eyes with cellular infiltration of the vitreous body are common with inflammations of the posterior eye segment. In mild course of the disease the local signs in the regions of inflammation are discrete; under these circumstances recurrences and remissions of the inflammation may be difficult to be differentiated. Several types of such an inflammation are described.", "contents": "[Discrete inflammatory manifestations of the posterior eye segment (author's transl)]. White eyes with cellular infiltration of the vitreous body are common with inflammations of the posterior eye segment. In mild course of the disease the local signs in the regions of inflammation are discrete; under these circumstances recurrences and remissions of the inflammation may be difficult to be differentiated. Several types of such an inflammation are described."}
{"id": "PMID:480804", "title": "[Disturbing physiologic diplopia (author's transl)].", "content": "Spontaneously observed physiologic diplopia seems to be a pathologic condition caused by a disturbance of high cerebral functions, especially of attention. In a personally observed case an organic psychosyndrome was present. The aetiology and an effective therapy are still unknown. Further studies of similar cases may promote explanation.", "contents": "[Disturbing physiologic diplopia (author's transl)]. Spontaneously observed physiologic diplopia seems to be a pathologic condition caused by a disturbance of high cerebral functions, especially of attention. In a personally observed case an organic psychosyndrome was present. The aetiology and an effective therapy are still unknown. Further studies of similar cases may promote explanation."}
{"id": "PMID:480805", "title": "[Comparative refractioning in juvenile strabismus patients. Experience with the Auto-Refractor 6600 (author's transl)].", "content": "122 strabismus patients have been refracted in order to compare the cycloplegic effect of Cyclopentolat-HCl (Zyklolat) and atropin. In young patients the cycloplegic effect of Zyklolat is not as sufficient as that of atropin is. Moreover the positions of cylindrical axes show differences up to 30 degrees under Zyklolat in comparison with atropin. On the other hand we give a report of our experience using the Auto-Refractor 6600, the before-mentioned measurings were performed with. -- The important field of the Auto-Refractor is the routine refractioning of co-operative patients. Using it on young children which are unco-operative the Auto-Refractor is not as sufficient as we hoped it to be.", "contents": "[Comparative refractioning in juvenile strabismus patients. Experience with the Auto-Refractor 6600 (author's transl)]. 122 strabismus patients have been refracted in order to compare the cycloplegic effect of Cyclopentolat-HCl (Zyklolat) and atropin. In young patients the cycloplegic effect of Zyklolat is not as sufficient as that of atropin is. Moreover the positions of cylindrical axes show differences up to 30 degrees under Zyklolat in comparison with atropin. On the other hand we give a report of our experience using the Auto-Refractor 6600, the before-mentioned measurings were performed with. -- The important field of the Auto-Refractor is the routine refractioning of co-operative patients. Using it on young children which are unco-operative the Auto-Refractor is not as sufficient as we hoped it to be."}
{"id": "PMID:480807", "title": "[An unusual case of Marcus Gunn phenomenon].", "content": "The author gives a short review on the course of the disease and the typical symptoms of regular Marcus Gunn phenomenon i.e. the retraction of an usually ptotic lid when the patient opens his mouth or moves his jaw to the healthy side of his face. He then reports on a boy now aged 3 and a half that had suffered from Marcus Gunn phenomenon since his birth, although the disease almost disappeared in the first years of his life. Only a short time before the parents noticed again the phenomenon, occurring now in a modified version; the retraction of the lid appears mainly then when the child pulls himself up from a lying position or raises the arm of this side where the ptosis is located.", "contents": "[An unusual case of Marcus Gunn phenomenon]. The author gives a short review on the course of the disease and the typical symptoms of regular Marcus Gunn phenomenon i.e. the retraction of an usually ptotic lid when the patient opens his mouth or moves his jaw to the healthy side of his face. He then reports on a boy now aged 3 and a half that had suffered from Marcus Gunn phenomenon since his birth, although the disease almost disappeared in the first years of his life. Only a short time before the parents noticed again the phenomenon, occurring now in a modified version; the retraction of the lid appears mainly then when the child pulls himself up from a lying position or raises the arm of this side where the ptosis is located."}
{"id": "PMID:480808", "title": "[Blindness following orbital haemorrhage after head injuries (author's transl)].", "content": "The sever orbital haemorrhage (Cords) can follow a contusion of the orbit. Only an immediate orbital decompression by Killian's incision provides permanent loss of vision. Report of seventeen own cases.", "contents": "[Blindness following orbital haemorrhage after head injuries (author's transl)]. The sever orbital haemorrhage (Cords) can follow a contusion of the orbit. Only an immediate orbital decompression by Killian's incision provides permanent loss of vision. Report of seventeen own cases."}
{"id": "PMID:480809", "title": "[The significance of vitrectomy after perforating injuries].", "content": "This paper presents three cases that show the importance of vitrectomy after perforating injuries of the eye. Whenever a trauma produces a mixing of vitreous and lens material in the anterior chamber, vitrectomy is the method of choice. Chronic posttraumatic uveitis can be prevented through this procedure. The three young patients, who were operated with the Vitrektor, reached a 6/6 vision with contact lenses after 1 1/2, 2 and 3 years respectively of observation. No consent can be given to the opinion recently expressed that the removal of infected vitreous after a cataract extraction nearly by an irrigation of the anterior chamber is considered to be a vitrectomy performed for decades. Priority for vitrectomy should be given to Kasner.", "contents": "[The significance of vitrectomy after perforating injuries]. This paper presents three cases that show the importance of vitrectomy after perforating injuries of the eye. Whenever a trauma produces a mixing of vitreous and lens material in the anterior chamber, vitrectomy is the method of choice. Chronic posttraumatic uveitis can be prevented through this procedure. The three young patients, who were operated with the Vitrektor, reached a 6/6 vision with contact lenses after 1 1/2, 2 and 3 years respectively of observation. No consent can be given to the opinion recently expressed that the removal of infected vitreous after a cataract extraction nearly by an irrigation of the anterior chamber is considered to be a vitrectomy performed for decades. Priority for vitrectomy should be given to Kasner."}
{"id": "PMID:480810", "title": "[Evaluation of the degree of disability for the privately insured patient (author's transl)].", "content": "There are marked differences between the public health system and the private insurance system. This paper shows how to evaluate the degree of permanent disability according to \"The General Rules for Private Insurances (AUVB 1965)\". At the beginning the evaluation of the degree of permanent disability after the visus naturalis or the visus corrected and at unilateral and bilateral aphakia will be discussed. The paper gives detailed information on the degree of disability for: one injured eye, one injured eye with preexisting ocular lesion, bilateral injury, one injured eye with preexisting lesion of the other eye, one injured eye with preexisting ocular lesions of both eyes, bilateral injury with preexisting lesions of both eyes. For bilateral lesions, the degree of disability is calculated as a function of the reduction in activity by the AUVB 1965; this is given in one table. The rules applying to Austria are compared to those valid in the Federal Republic of Germany (AUB). It is very important to understand these methods of calculation, if one wants to secure an objective ophthalmological evaluation of the degree of disability for the privately insured patient.", "contents": "[Evaluation of the degree of disability for the privately insured patient (author's transl)]. There are marked differences between the public health system and the private insurance system. This paper shows how to evaluate the degree of permanent disability according to \"The General Rules for Private Insurances (AUVB 1965)\". At the beginning the evaluation of the degree of permanent disability after the visus naturalis or the visus corrected and at unilateral and bilateral aphakia will be discussed. The paper gives detailed information on the degree of disability for: one injured eye, one injured eye with preexisting ocular lesion, bilateral injury, one injured eye with preexisting lesion of the other eye, one injured eye with preexisting ocular lesions of both eyes, bilateral injury with preexisting lesions of both eyes. For bilateral lesions, the degree of disability is calculated as a function of the reduction in activity by the AUVB 1965; this is given in one table. The rules applying to Austria are compared to those valid in the Federal Republic of Germany (AUB). It is very important to understand these methods of calculation, if one wants to secure an objective ophthalmological evaluation of the degree of disability for the privately insured patient."}
{"id": "PMID:480811", "title": "[Malignant melanoma of the choroid in melanosis bulbi (author's transl)].", "content": "Report of a case with melanosis bulbi observed in a relatively young patient of 33 years, in which a malignant melanoma of the choroid developed. As reported in earlier examinations the incidence of malignant melanoma in cases of melanosis bulbi is much higher than in normal eyes. The authors recommend strongly routine biomicroscopic examinations in cases of melanosis bulbi every 1--2 years to recognize the development of a melanoma in an early stage.", "contents": "[Malignant melanoma of the choroid in melanosis bulbi (author's transl)]. Report of a case with melanosis bulbi observed in a relatively young patient of 33 years, in which a malignant melanoma of the choroid developed. As reported in earlier examinations the incidence of malignant melanoma in cases of melanosis bulbi is much higher than in normal eyes. The authors recommend strongly routine biomicroscopic examinations in cases of melanosis bulbi every 1--2 years to recognize the development of a melanoma in an early stage."}
{"id": "PMID:480812", "title": "[Diagnostics of intraocular tumors with the radiophosphorus test (author's transl)].", "content": "The author reports about the radio-phosphorus-test as an additional method in diagnostics of intraocular tumors in 62 examinations. The conformity between 49 histologically examined cases and the positive test-results amounted to 90%.", "contents": "[Diagnostics of intraocular tumors with the radiophosphorus test (author's transl)]. The author reports about the radio-phosphorus-test as an additional method in diagnostics of intraocular tumors in 62 examinations. The conformity between 49 histologically examined cases and the positive test-results amounted to 90%."}
{"id": "PMID:480813", "title": "[Epibulbar tumours (author's transl)].", "content": "After a classification in benign and malignant tumours and in pigmented and non-pigmented growth, attention is drawn to the fact that most of the tumours lie in the palpebral fissure and are benign. Leukoplakia is a clinical description but not a diagnosis. Both the benign and the malignant growths can be called Leukoplakia. The intra-epithelial epithelioma is often incorrectly diagnosed as Bowen's disease. This disease of the skin and the intraepithelial epithelioma of the limbus are histologically quite different, and therefore the term Bowen's disease is incorrect. Not all the various tumours occur with the same incidence, e. g. the squamous cell carcinoma occurs more often in some countries than in others. The malignant melanomas of the conjunctiva are not so malignant as in the caruncle or cornea. In the conjunctiva secundary melanomas can occur after penetration of an intraocular melanoma through the sclera; similary metastases can occur in the form of epibulbar tumours. In all cases the epibulbar tumour should be excised very carefully and a histological examination is necessary for the diagnosis.", "contents": "[Epibulbar tumours (author's transl)]. After a classification in benign and malignant tumours and in pigmented and non-pigmented growth, attention is drawn to the fact that most of the tumours lie in the palpebral fissure and are benign. Leukoplakia is a clinical description but not a diagnosis. Both the benign and the malignant growths can be called Leukoplakia. The intra-epithelial epithelioma is often incorrectly diagnosed as Bowen's disease. This disease of the skin and the intraepithelial epithelioma of the limbus are histologically quite different, and therefore the term Bowen's disease is incorrect. Not all the various tumours occur with the same incidence, e. g. the squamous cell carcinoma occurs more often in some countries than in others. The malignant melanomas of the conjunctiva are not so malignant as in the caruncle or cornea. In the conjunctiva secundary melanomas can occur after penetration of an intraocular melanoma through the sclera; similary metastases can occur in the form of epibulbar tumours. In all cases the epibulbar tumour should be excised very carefully and a histological examination is necessary for the diagnosis."}
{"id": "PMID:480814", "title": "[Electronmicroscopic studies of the trabecular meshwork in corticosteroid glaucoma].", "content": "The tissue specimen of a 41-year-old patient with cortisonglaucoma, obtained by trabeculectomy, was examined by electronmicroscope. There are massive depositions of band-like and linear arranged structures in the cribriform area and adjacent lamellae of the trabeculum corneosclerale. The pathways for aqueous humor of this region are almost completely obstructed by this material. That is the reason for an increased outflow resistance. The cells of this area show signs of degeneration and are reduced in their numbers. -- The cortisonglaucoma differs from all the other forms of glaucoma especially from glaucoma simplex by these characteristic deposits.", "contents": "[Electronmicroscopic studies of the trabecular meshwork in corticosteroid glaucoma]. The tissue specimen of a 41-year-old patient with cortisonglaucoma, obtained by trabeculectomy, was examined by electronmicroscope. There are massive depositions of band-like and linear arranged structures in the cribriform area and adjacent lamellae of the trabeculum corneosclerale. The pathways for aqueous humor of this region are almost completely obstructed by this material. That is the reason for an increased outflow resistance. The cells of this area show signs of degeneration and are reduced in their numbers. -- The cortisonglaucoma differs from all the other forms of glaucoma especially from glaucoma simplex by these characteristic deposits."}
{"id": "PMID:480815", "title": "[Trabeculetomy-iridencleisis with marginal cyclodialysis in the treatment of selected cases of childhood- and juvenile glaucoma].", "content": "This surgery method is thought for cases, where previous operations have not been successful or for such, in which severe scaring tissue reactions must be expected. The diaphanoscopy of the anterior chamber angle is considered helpfull for the choice of the surgery way. Between 1975 and february 1978 trabeculectomy combined with radical lobelike iridencleisis and marginal cyclodialyse was performed in 4 cases and the i.o. pressure could be normalised. Complications did not occure.", "contents": "[Trabeculetomy-iridencleisis with marginal cyclodialysis in the treatment of selected cases of childhood- and juvenile glaucoma]. This surgery method is thought for cases, where previous operations have not been successful or for such, in which severe scaring tissue reactions must be expected. The diaphanoscopy of the anterior chamber angle is considered helpfull for the choice of the surgery way. Between 1975 and february 1978 trabeculectomy combined with radical lobelike iridencleisis and marginal cyclodialyse was performed in 4 cases and the i.o. pressure could be normalised. Complications did not occure."}
{"id": "PMID:480816", "title": "[Clinical experience in glaucoma surgery utilizing silicon catheters (author's transl)].", "content": "In 7 probands exhibiting haemorrhagic glaucoma a combined thermosclerostomy with insertion of a silicons tubing (with or without basal iridectomy) has been performed. The catheter had an inner diameter of 0,5 mm, measuring about 5 mm in length and projecting about 1,5 mm into the anterior chamber. The tubing has been anchored proximally with two nylon sutures (10--0) to the corneal wound edge going through full thickness cornea; the distal end has been anchored with two more nylon sutures to underlying sclera. Tenon's capsules and conjunctiva were closed separately. In all cases the tubing has been well tolerated and did not exhibit any sign of displacement, the intracameral opening remained patent. During an observation period of 3--16 months with or without additional antiglaucomatous medication an intraocular pressure between 14--22 mm Hg could be achieved; all patients remained asymptomatic.", "contents": "[Clinical experience in glaucoma surgery utilizing silicon catheters (author's transl)]. In 7 probands exhibiting haemorrhagic glaucoma a combined thermosclerostomy with insertion of a silicons tubing (with or without basal iridectomy) has been performed. The catheter had an inner diameter of 0,5 mm, measuring about 5 mm in length and projecting about 1,5 mm into the anterior chamber. The tubing has been anchored proximally with two nylon sutures (10--0) to the corneal wound edge going through full thickness cornea; the distal end has been anchored with two more nylon sutures to underlying sclera. Tenon's capsules and conjunctiva were closed separately. In all cases the tubing has been well tolerated and did not exhibit any sign of displacement, the intracameral opening remained patent. During an observation period of 3--16 months with or without additional antiglaucomatous medication an intraocular pressure between 14--22 mm Hg could be achieved; all patients remained asymptomatic."}
{"id": "PMID:480817", "title": "[Fluorescein angiographic studies of the effect of Harms' and Dannheim's trabeculotomia ab externo].", "content": "The mechanism of the outflow of the aqueous humor was investigated on 9 eyes with open angle glaucoma operated by traveculotomia ab externo. The anterior chamber was filled with 0,2% Fluorescein-Ringer-solution, before and after the operation. Comparing the fluorescein angiographic pictures it can be concluded that the effect of the operation it caused by an improvement of the outflow of the aequous humor through the system of Schlemm's canal as well as through the formation of a fistula.", "contents": "[Fluorescein angiographic studies of the effect of Harms' and Dannheim's trabeculotomia ab externo]. The mechanism of the outflow of the aqueous humor was investigated on 9 eyes with open angle glaucoma operated by traveculotomia ab externo. The anterior chamber was filled with 0,2% Fluorescein-Ringer-solution, before and after the operation. Comparing the fluorescein angiographic pictures it can be concluded that the effect of the operation it caused by an improvement of the outflow of the aequous humor through the system of Schlemm's canal as well as through the formation of a fistula."}
{"id": "PMID:480818", "title": "[Massage of the bulb and intraocular pressure after goniotrepanation (author's transl)].", "content": "Intraocular pressure should be controlled via applanation tonometry, beginning with the fourth day after goniotrepanation, for a period of about three weeks, for early detection of postoperative pressure increases which can occur occasionally, so that they may be treated by digital massage of the bulb. This can be effective even in later post-operative pressure increases and support the effect of additional pressure-lowering treatment.", "contents": "[Massage of the bulb and intraocular pressure after goniotrepanation (author's transl)]. Intraocular pressure should be controlled via applanation tonometry, beginning with the fourth day after goniotrepanation, for a period of about three weeks, for early detection of postoperative pressure increases which can occur occasionally, so that they may be treated by digital massage of the bulb. This can be effective even in later post-operative pressure increases and support the effect of additional pressure-lowering treatment."}
{"id": "PMID:480819", "title": "[Experience in therapy and prophylaxis of epidemic keratoconjunctivitis (author's transl)].", "content": "101 patients with epidemic keratoconjunctivitis were treated with different eye drops: cortisone, antibiotics and P.V.P.-Iodine. The treatment of 19 patients with P.V.P.-Iodine showed that inflammatory symptoms disappeared rapidly; corneal complications however such as superficial keratitis could not be prevented. After the outbreak of epidemic keratoconjunctivitis, severe hygienic measures had been taken at the eye-clinic. On account of the hygienic prophylactic measures further infections could be prevented at the clinic almost completely.", "contents": "[Experience in therapy and prophylaxis of epidemic keratoconjunctivitis (author's transl)]. 101 patients with epidemic keratoconjunctivitis were treated with different eye drops: cortisone, antibiotics and P.V.P.-Iodine. The treatment of 19 patients with P.V.P.-Iodine showed that inflammatory symptoms disappeared rapidly; corneal complications however such as superficial keratitis could not be prevented. After the outbreak of epidemic keratoconjunctivitis, severe hygienic measures had been taken at the eye-clinic. On account of the hygienic prophylactic measures further infections could be prevented at the clinic almost completely."}
{"id": "PMID:480820", "title": "[Ultrastructural changes in keratoconus].", "content": "In case of keratoconus the epithelial cells are also participating in the pathological processes. Their activity of producing basal membrane is increased. In the stroma four cell types are found; two of them are normal elements. The origin of the two pathological forms is unknown. They may represent as well vagrant cells. Or they originate perhaps from the endothelial cells, being more or less modified. The original endothelial cells are mostly destroyed. In all sorts of the corneal cells one sees abundantly enlarged mitochondria.", "contents": "[Ultrastructural changes in keratoconus]. In case of keratoconus the epithelial cells are also participating in the pathological processes. Their activity of producing basal membrane is increased. In the stroma four cell types are found; two of them are normal elements. The origin of the two pathological forms is unknown. They may represent as well vagrant cells. Or they originate perhaps from the endothelial cells, being more or less modified. The original endothelial cells are mostly destroyed. In all sorts of the corneal cells one sees abundantly enlarged mitochondria."}
{"id": "PMID:480822", "title": "[Utilization of the vitreophage in cataract surgery. Juvenile and traumatic cataract, postcataract membranes].", "content": "15 cases of congenital and juvenile cataract have been operated on utilizing the vitreophage of Kl\u00f6ti. In 3 instances posttraumatic cataracts have been removed, in two cases a cataracta complicata, in 9 cases after-cataractous membranes. Serious complications have not been encountered in any of our cases. One patients with congenital cataract and two probands with cataracta complicata developed postoperatively considerable flare in the anterior chamber and vitreous body, possibly the result of a phacolytic reaction. Excision of thick, inelastic after cataractous membranes proved to be difficult at times. The advantage of lensectomy lies in the possiblity of removal at the same sitting of opaque vitreous structures which had become visible during surgery.", "contents": "[Utilization of the vitreophage in cataract surgery. Juvenile and traumatic cataract, postcataract membranes]. 15 cases of congenital and juvenile cataract have been operated on utilizing the vitreophage of Kl\u00f6ti. In 3 instances posttraumatic cataracts have been removed, in two cases a cataracta complicata, in 9 cases after-cataractous membranes. Serious complications have not been encountered in any of our cases. One patients with congenital cataract and two probands with cataracta complicata developed postoperatively considerable flare in the anterior chamber and vitreous body, possibly the result of a phacolytic reaction. Excision of thick, inelastic after cataractous membranes proved to be difficult at times. The advantage of lensectomy lies in the possiblity of removal at the same sitting of opaque vitreous structures which had become visible during surgery."}
{"id": "PMID:480823", "title": "[Diabetic retinopathy: 10 years after light-coagulation (author's transl)].", "content": "A retrospective study of 19 patients, who suffered from diabetic retinopathy, were submitted to a xenon-light-coagulation 10 years ago and have been regularly checked, shows better results in the treated eyes than in the untreated fellow-eyes in regard to visual acuity and background morphology. In cases of poorly controlled metabolism, heavy smoking and simultaneous suffering from hypertension and nephropathy the prognosis of the treated eyes is unfavourably affected.", "contents": "[Diabetic retinopathy: 10 years after light-coagulation (author's transl)]. A retrospective study of 19 patients, who suffered from diabetic retinopathy, were submitted to a xenon-light-coagulation 10 years ago and have been regularly checked, shows better results in the treated eyes than in the untreated fellow-eyes in regard to visual acuity and background morphology. In cases of poorly controlled metabolism, heavy smoking and simultaneous suffering from hypertension and nephropathy the prognosis of the treated eyes is unfavourably affected."}
{"id": "PMID:480824", "title": "[Fundus changes due to arteriosclerosis and chronic-arterial hypertension (author's transl)].", "content": "The authors report on difficulties in ophthalmoscopac differential diagnosis of fundus changes due to arteriosclerosis and hypertension. An attempt is made to present a classification of hypertensive alterations which -- in close association with the scheme published by Keith, Wagner and Neubauer -- uses the same descriptive terms but restricts itself to distinguish between changes of the vascular system on the one hand and changes of the parenchym as a consequence of the vascular changes on the other hand.", "contents": "[Fundus changes due to arteriosclerosis and chronic-arterial hypertension (author's transl)]. The authors report on difficulties in ophthalmoscopac differential diagnosis of fundus changes due to arteriosclerosis and hypertension. An attempt is made to present a classification of hypertensive alterations which -- in close association with the scheme published by Keith, Wagner and Neubauer -- uses the same descriptive terms but restricts itself to distinguish between changes of the vascular system on the one hand and changes of the parenchym as a consequence of the vascular changes on the other hand."}
{"id": "PMID:480825", "title": "[Remarkable cases of temporal arteritis].", "content": "Blood-circulatory disorders and their end results at the fundus of the eye must always draw our attention to a possible Arteriitis temporalis Horton, even if there are no general symptoms, when the clinical picture disguises the vascular disease, or even when an important symptom of the disease, such as the accelerated basal sedimentation rate, is missing.", "contents": "[Remarkable cases of temporal arteritis]. Blood-circulatory disorders and their end results at the fundus of the eye must always draw our attention to a possible Arteriitis temporalis Horton, even if there are no general symptoms, when the clinical picture disguises the vascular disease, or even when an important symptom of the disease, such as the accelerated basal sedimentation rate, is missing."}
{"id": "PMID:480826", "title": "[Electroophthalmologic studies of papillitis, vascular papilledema and obstructed papilla].", "content": "The photopic electroretinogram (ERG) and the brightness visually evoked cortical potentials (HEPs) were recorded from patients with oedema of the optic disc caused by papillitis (7 patients), by vascular disease (10 patients) and by intracranial hypertension. For the ERG a Henkes-electrode in reference to an earlobe electrode was used. The H-EPs were recorded bipolar in the midline 5% to 25% and unipolar 15% in reference to an earlobe electrode (inion to nasion = 100%). For evaluation the amplitudes and the peaktimes of the a- and b-wave of the ERG were measured. In the H-EPs the amplitudes and the peaktimes of a negatives peak at approximately 70 ms (NGZ) and a positive peak at approximately 120 ms (PGZ) were obtained from the bipolar recording and a positive peak at approximately 100 ms (PGZ) and a negative peak at approximately 140 ms (NGZ) obtained from the unipolar recording were measured. The photopic ERGs of the affected eye showed normal values, however the H-EPs were distinctly pahtological. With the exception of the negative peaktime (NGZ) of the bipolar leads of the intracranial hypertensive papiloedema, all peaktimes were lengthened and amplitudes decreased. These findings were especially evident in the bipolar tracings of papillitis and unipolar tracings of intracranial hypertension.", "contents": "[Electroophthalmologic studies of papillitis, vascular papilledema and obstructed papilla]. The photopic electroretinogram (ERG) and the brightness visually evoked cortical potentials (HEPs) were recorded from patients with oedema of the optic disc caused by papillitis (7 patients), by vascular disease (10 patients) and by intracranial hypertension. For the ERG a Henkes-electrode in reference to an earlobe electrode was used. The H-EPs were recorded bipolar in the midline 5% to 25% and unipolar 15% in reference to an earlobe electrode (inion to nasion = 100%). For evaluation the amplitudes and the peaktimes of the a- and b-wave of the ERG were measured. In the H-EPs the amplitudes and the peaktimes of a negatives peak at approximately 70 ms (NGZ) and a positive peak at approximately 120 ms (PGZ) were obtained from the bipolar recording and a positive peak at approximately 100 ms (PGZ) and a negative peak at approximately 140 ms (NGZ) obtained from the unipolar recording were measured. The photopic ERGs of the affected eye showed normal values, however the H-EPs were distinctly pahtological. With the exception of the negative peaktime (NGZ) of the bipolar leads of the intracranial hypertensive papiloedema, all peaktimes were lengthened and amplitudes decreased. These findings were especially evident in the bipolar tracings of papillitis and unipolar tracings of intracranial hypertension."}
{"id": "PMID:480827", "title": "[Differential diagnosis of amblyopia and retrobulbar neuritis with electroophthalmologic methods].", "content": "The electroretinogram (ERG), the brightness visual evoked cortical potential (H-EP) and the pattern reversal visual evoked cortical potential (M-EP) were recorded from 7 patients with amblyopia and 5 patients with neuritis retrobulbaris. The ERGs and the H-EPs of the amblyopic eyes were normal whereas the amplitude of the M-EP was significant diminished. The parameters of the ERG recorded from the patients with neuritis retrobulbaris showed normal values, however the H-EPs were significantly decreased and the peaktimes were delayed. Also a decrease in the amplitudes of the M-EPs was noticed. This method is a usefull clinical contribution in the differential diagnosis between amblyopia and neuritis retrobulbaris.", "contents": "[Differential diagnosis of amblyopia and retrobulbar neuritis with electroophthalmologic methods]. The electroretinogram (ERG), the brightness visual evoked cortical potential (H-EP) and the pattern reversal visual evoked cortical potential (M-EP) were recorded from 7 patients with amblyopia and 5 patients with neuritis retrobulbaris. The ERGs and the H-EPs of the amblyopic eyes were normal whereas the amplitude of the M-EP was significant diminished. The parameters of the ERG recorded from the patients with neuritis retrobulbaris showed normal values, however the H-EPs were significantly decreased and the peaktimes were delayed. Also a decrease in the amplitudes of the M-EPs was noticed. This method is a usefull clinical contribution in the differential diagnosis between amblyopia and neuritis retrobulbaris."}
{"id": "PMID:480828", "title": "[Electroophthalmologic and fluorescein angiographic studies in a case of exudative retinal detachment with spontaneous healing].", "content": "Electroophthalmological and fluorescein angiographic studies were performed on a 13 year old girl with exsudative detachment of the retina during a hypertensive crisis. The ERG showed a significant reduction during the course of the detachment. After healing the ERG values had improved as compared to those values recorded during the acute illness, but never reached normal levels. The lesions of the retina and the pigment epithelium caused by the detachment were responsible for pathological EOG. The fluorescein angiography showed circumscript and conglomerate lesions in the pigment epithelium.", "contents": "[Electroophthalmologic and fluorescein angiographic studies in a case of exudative retinal detachment with spontaneous healing]. Electroophthalmological and fluorescein angiographic studies were performed on a 13 year old girl with exsudative detachment of the retina during a hypertensive crisis. The ERG showed a significant reduction during the course of the detachment. After healing the ERG values had improved as compared to those values recorded during the acute illness, but never reached normal levels. The lesions of the retina and the pigment epithelium caused by the detachment were responsible for pathological EOG. The fluorescein angiography showed circumscript and conglomerate lesions in the pigment epithelium."}
{"id": "PMID:480829", "title": "[The morphology of the iris (author's transl)].", "content": "Scanning electron microscopy provides new information about the ultrastructure of the iris. The anterior surface of the iris, oriented towards the anterior chamber, is covered with a single layer of mesenchymal cells. Openings between the dendrite-like branching extensions of these cells form communicating channels between anterior chamber and stroma. The collagen fibres of the iris stroma are organized as rather tightly packed two-dimensional networks. The meshes of these networks form channels, oriented in an anterior-posterior direction, reaching the vessels of the iris. These vessels are wrapped by a tightly packed fibrous coating, consisting of collagen fibres which embrace the vessels in a helical fashion. It is assumed that the transiridial convection current acts as part of a temperature regulating mechanism.", "contents": "[The morphology of the iris (author's transl)]. Scanning electron microscopy provides new information about the ultrastructure of the iris. The anterior surface of the iris, oriented towards the anterior chamber, is covered with a single layer of mesenchymal cells. Openings between the dendrite-like branching extensions of these cells form communicating channels between anterior chamber and stroma. The collagen fibres of the iris stroma are organized as rather tightly packed two-dimensional networks. The meshes of these networks form channels, oriented in an anterior-posterior direction, reaching the vessels of the iris. These vessels are wrapped by a tightly packed fibrous coating, consisting of collagen fibres which embrace the vessels in a helical fashion. It is assumed that the transiridial convection current acts as part of a temperature regulating mechanism."}
{"id": "PMID:480830", "title": "[The effects of the xenon arc lamp upon the iris of the pigmented rabbit eye (author's transl)].", "content": "The ultrastructural alterations of 10 pigmented rabbit irides 15 minutes to 257 days after irradiation with a xenon arc lamp were analysed. Pulse duration was varied while irradiation power was kept constant at 3.24 W. 0.32 J (= 100 ms pulse duration) were delivered to the left eye and 3.2 J (= 1 second pulse duration) to the right side. The area of damage event was considerably smaller at the lower energy level. However depth of the crater was the same at both higher and lower levels. Cells and organelles disintegrate forming lamellar structures and coarse granules. The shape of the pigment granules remains intact. Collagen fibres disintegrate into subunits loosing their periodicity. Mesenchymal cells cover the area of damage few days after irradiation at the low energy level. A thin scar remains at the impact site. High energy irradiation shifts the equilibrium towards degenerative phenomena. Secondary degenerative processes are powerful enough to induce a total decay of the iris substance. The reactivity of the iris irradiated by a xenon arc lamp thus depends from either irradiation duration, power level or from both (energy).", "contents": "[The effects of the xenon arc lamp upon the iris of the pigmented rabbit eye (author's transl)]. The ultrastructural alterations of 10 pigmented rabbit irides 15 minutes to 257 days after irradiation with a xenon arc lamp were analysed. Pulse duration was varied while irradiation power was kept constant at 3.24 W. 0.32 J (= 100 ms pulse duration) were delivered to the left eye and 3.2 J (= 1 second pulse duration) to the right side. The area of damage event was considerably smaller at the lower energy level. However depth of the crater was the same at both higher and lower levels. Cells and organelles disintegrate forming lamellar structures and coarse granules. The shape of the pigment granules remains intact. Collagen fibres disintegrate into subunits loosing their periodicity. Mesenchymal cells cover the area of damage few days after irradiation at the low energy level. A thin scar remains at the impact site. High energy irradiation shifts the equilibrium towards degenerative phenomena. Secondary degenerative processes are powerful enough to induce a total decay of the iris substance. The reactivity of the iris irradiated by a xenon arc lamp thus depends from either irradiation duration, power level or from both (energy)."}
{"id": "PMID:480831", "title": "[The effects of a gigant-pulse-laser upon the iris of the pigmented rabbit eye (author's transl)].", "content": "The eyes of 10 pigmented rabbits were irradiated by a Q-switched Nd:YAG laser. The pulse energy delivered to the right eye was 187 mJ while the left eye was irradiated with 58 mJ. The pulse duration in both cases was 25 ns. The destructive effects were analysed for two different positions of the focus. In the upper half of the eye the focus was positioned at the anterior iris surface. In the lower half it was adjusted near the pigment epithelium. The eyes were analysed by electron microscopy at intervals of 15 minutes to 128 days after irradiation. The immediate effects were found to depend from the position of the focus but less from pulse energy. At later phases, tissue reaction depends from pulse energy. -- Scarification, at the lower energy level, is almost complete after 32 days. At the higher energy level, degenerative processi are setting in; 3 days after irradiation a progressing iris atrophy is observed which has not yet faded at the 128th day.", "contents": "[The effects of a gigant-pulse-laser upon the iris of the pigmented rabbit eye (author's transl)]. The eyes of 10 pigmented rabbits were irradiated by a Q-switched Nd:YAG laser. The pulse energy delivered to the right eye was 187 mJ while the left eye was irradiated with 58 mJ. The pulse duration in both cases was 25 ns. The destructive effects were analysed for two different positions of the focus. In the upper half of the eye the focus was positioned at the anterior iris surface. In the lower half it was adjusted near the pigment epithelium. The eyes were analysed by electron microscopy at intervals of 15 minutes to 128 days after irradiation. The immediate effects were found to depend from the position of the focus but less from pulse energy. At later phases, tissue reaction depends from pulse energy. -- Scarification, at the lower energy level, is almost complete after 32 days. At the higher energy level, degenerative processi are setting in; 3 days after irradiation a progressing iris atrophy is observed which has not yet faded at the 128th day."}
{"id": "PMID:480832", "title": "[The EMI-scanner CT 5005 for exploring all organs the eye and the orbit (author's transl)].", "content": "The EMI-scanner CT 5005 is well suited for exploring all the organs of the body, from top to toe. Its advantages, particularly in ophtalmology, are discussed: quality of imaging not only brain and other soft organs, but bones as well; precise analysis of the base of skull with the matrix 320X320; possibility of primary coronal section. The machine has proven very successful in the study of the orbits, their content and surroundings.", "contents": "[The EMI-scanner CT 5005 for exploring all organs the eye and the orbit (author's transl)]. The EMI-scanner CT 5005 is well suited for exploring all the organs of the body, from top to toe. Its advantages, particularly in ophtalmology, are discussed: quality of imaging not only brain and other soft organs, but bones as well; precise analysis of the base of skull with the matrix 320X320; possibility of primary coronal section. The machine has proven very successful in the study of the orbits, their content and surroundings."}
{"id": "PMID:480833", "title": "[Computerized tomography of the orbita (author's transl)].", "content": "Computerized tomography of the orbita is today the most accurate method to determine the causes of exophthalmus. It can detect and localise precisely congenital, inflammatory and neoplastic disease of the orbita and its surroundings. Specific diagnosis is not always possible; it may be attained, if complementary neuroradiological methods are applied. Higher spatial resolution will probably allow to apply computerized tomography also to other, more discrete orbital diseases.", "contents": "[Computerized tomography of the orbita (author's transl)]. Computerized tomography of the orbita is today the most accurate method to determine the causes of exophthalmus. It can detect and localise precisely congenital, inflammatory and neoplastic disease of the orbita and its surroundings. Specific diagnosis is not always possible; it may be attained, if complementary neuroradiological methods are applied. Higher spatial resolution will probably allow to apply computerized tomography also to other, more discrete orbital diseases."}
{"id": "PMID:480834", "title": "[Combined use of computerized tomography and echography in ophthalmology (author's transl)].", "content": "Computerized tomography does not give as fine an information as echography in the presence of intraocular changes, such as retinal detachments, organized vitreous hemorrhages or the differentiation of a choroidal prominence. Complementary computerized tomography, however, can be helpful in the presence of unclear echographic results in opaque media. Furthermore, an analysis of the value of computerized tomography and echography for the evaluation of orbital lesions is presented based mainly on the results of the preoperative tissue diagnosis in 31 histological proven lesions. The results show that a complementary CT-scan is not necessary in orbital lesions of the anterior third when a clear echographic tissue diagnosis exists. In lesions of the middle third of the orbit, echography and CT complement each other. For lesions situated within the orbital apex or for those which are in connection with adjacent structures, such as the intracranial space or the periorbital region, computerized tomography is superior and of a greater help than standardized A-scan echography aided by contact B-scan.", "contents": "[Combined use of computerized tomography and echography in ophthalmology (author's transl)]. Computerized tomography does not give as fine an information as echography in the presence of intraocular changes, such as retinal detachments, organized vitreous hemorrhages or the differentiation of a choroidal prominence. Complementary computerized tomography, however, can be helpful in the presence of unclear echographic results in opaque media. Furthermore, an analysis of the value of computerized tomography and echography for the evaluation of orbital lesions is presented based mainly on the results of the preoperative tissue diagnosis in 31 histological proven lesions. The results show that a complementary CT-scan is not necessary in orbital lesions of the anterior third when a clear echographic tissue diagnosis exists. In lesions of the middle third of the orbit, echography and CT complement each other. For lesions situated within the orbital apex or for those which are in connection with adjacent structures, such as the intracranial space or the periorbital region, computerized tomography is superior and of a greater help than standardized A-scan echography aided by contact B-scan."}
{"id": "PMID:480835", "title": "[Computerized tomography -- results in affections of the chiasm and visual pathway (author's transl)].", "content": "272 cases with visual field defects were examined by CT and analysed. 78 patients showed a chiasmatic lesion, 45 patients a lesion of the optic tract or the lateral geniculate body and 136 patients a lesion of the optic radiation or the occipital cortex. 96% of the CT pictures were topographically corresponding to the known optic field defect. In more then 75% of the operated patients the nature of the CT lesion was confirmed. 10 cases were negative in CT and 3 false positive. Because of the high rate of exact topographic diagnosis CT should be the initial investigation in visual field defects. But the nature of a lesion has to be diagnosed with caution, taking into account the entire clinical picture.", "contents": "[Computerized tomography -- results in affections of the chiasm and visual pathway (author's transl)]. 272 cases with visual field defects were examined by CT and analysed. 78 patients showed a chiasmatic lesion, 45 patients a lesion of the optic tract or the lateral geniculate body and 136 patients a lesion of the optic radiation or the occipital cortex. 96% of the CT pictures were topographically corresponding to the known optic field defect. In more then 75% of the operated patients the nature of the CT lesion was confirmed. 10 cases were negative in CT and 3 false positive. Because of the high rate of exact topographic diagnosis CT should be the initial investigation in visual field defects. But the nature of a lesion has to be diagnosed with caution, taking into account the entire clinical picture."}
{"id": "PMID:480836", "title": "[Axial computerised tomography - answered and unanswered questions on the ophthalmologist (author's transl)].", "content": "The results of 67 examinations with computerized axial tomography are presented with reference to the indications. 15 examinations were performed in exophthalmos, 11 in unknown visual field defects, 9 in optic atrophy of unknown origin, 8 each in optic neuritis, papilledema and unclear visual disturbance. 8 furthur examinations were performed in different cases. The axial computerized tomography proved to be an efficient complementary examination technique if it is limited to well defined indications. The best results are obtained examining exophthalmos and visual field defects.", "contents": "[Axial computerised tomography - answered and unanswered questions on the ophthalmologist (author's transl)]. The results of 67 examinations with computerized axial tomography are presented with reference to the indications. 15 examinations were performed in exophthalmos, 11 in unknown visual field defects, 9 in optic atrophy of unknown origin, 8 each in optic neuritis, papilledema and unclear visual disturbance. 8 furthur examinations were performed in different cases. The axial computerized tomography proved to be an efficient complementary examination technique if it is limited to well defined indications. The best results are obtained examining exophthalmos and visual field defects."}
{"id": "PMID:480837", "title": "[Computertomography for diagnosis of optic nerve and chiasmal gliomas (author's transl)].", "content": "Discussion of the clinical symptomatology of optic nerve and chiasmal gliomas based on personal observations. Evaluation of plain X-ray diagnostic procedures, A-scan ultrasonography and especially computertomography for diagnosis of optic nerve and chiasmal gliomas. Discussion of modern attitude in treatment of these tumors: surgical, irradiation therapy.", "contents": "[Computertomography for diagnosis of optic nerve and chiasmal gliomas (author's transl)]. Discussion of the clinical symptomatology of optic nerve and chiasmal gliomas based on personal observations. Evaluation of plain X-ray diagnostic procedures, A-scan ultrasonography and especially computertomography for diagnosis of optic nerve and chiasmal gliomas. Discussion of modern attitude in treatment of these tumors: surgical, irradiation therapy."}
{"id": "PMID:480838", "title": "[The computerized tomography in findings posttraumatic hematoma (author's transl)].", "content": "The authors report two cases of posttraumatic hematoma in the orbit. The first patient presents an unilateral amaurosis through indirect trauma of the optic nerve. The computerized tomography reveals a hematoma of the optic nerve sheath, confirmed by surgery. The second patient presents a posttraumatic limitation of the ocular motility and the computerized tomography reveals a hematoma of two extraocular muscles.", "contents": "[The computerized tomography in findings posttraumatic hematoma (author's transl)]. The authors report two cases of posttraumatic hematoma in the orbit. The first patient presents an unilateral amaurosis through indirect trauma of the optic nerve. The computerized tomography reveals a hematoma of the optic nerve sheath, confirmed by surgery. The second patient presents a posttraumatic limitation of the ocular motility and the computerized tomography reveals a hematoma of two extraocular muscles."}
{"id": "PMID:480839", "title": "[Computed tomography findings in bilateral blindness (author's transl)].", "content": "The use of computed tomography (CT) simplifies neuroradiological investigations. A CT helped to confirm the diagnosis of an occipital vascular leasion in two cases of bilateral blindness.", "contents": "[Computed tomography findings in bilateral blindness (author's transl)]. The use of computed tomography (CT) simplifies neuroradiological investigations. A CT helped to confirm the diagnosis of an occipital vascular leasion in two cases of bilateral blindness."}
{"id": "PMID:480840", "title": "[The search for the right place and for optimal fixation of an intraocular lens (author's transl)].", "content": "Whereas by far not all properties of the crystalline lens can be realized with an artifical intraocular lens, maximal stability of such a device is its most important feature. The latter can be achieved by the adherence of the lens to the capsular membrane, left behind after extra-capsular cataract extraction. In this way the suspension system of the crystalline is used for the fixation of the intra-ocular lens. The stability of the contents of the eye viz. of the vitreous, of the aqueous humour, and of the iris diaphragm, is maintained to a high degree. Stability of the iris diaphragma is achieved when irido-capsulo-lenticular adhesions are allowed to develop, but the iris diaphragm can also be stabilized by support from the optical part of a modified capsular lens in the posterior chamber.", "contents": "[The search for the right place and for optimal fixation of an intraocular lens (author's transl)]. Whereas by far not all properties of the crystalline lens can be realized with an artifical intraocular lens, maximal stability of such a device is its most important feature. The latter can be achieved by the adherence of the lens to the capsular membrane, left behind after extra-capsular cataract extraction. In this way the suspension system of the crystalline is used for the fixation of the intra-ocular lens. The stability of the contents of the eye viz. of the vitreous, of the aqueous humour, and of the iris diaphragm, is maintained to a high degree. Stability of the iris diaphragma is achieved when irido-capsulo-lenticular adhesions are allowed to develop, but the iris diaphragm can also be stabilized by support from the optical part of a modified capsular lens in the posterior chamber."}
{"id": "PMID:480841", "title": "[Results of lens implants in Dr. Binkhorst's patients (author's transl)].", "content": "Postoperative results of lens implants after extracapsular cataract extraction in 99 of Dr. Binkhorst's patients in the year 1976/77. Evaluation of functional and morphological results. The visibility of the fundus is seriously compromised in comparison to intracapsular extracted aphakes without implant. Preoperative calculation of the lens to be implanted will reduce post-operative ammetropy to a minimum.", "contents": "[Results of lens implants in Dr. Binkhorst's patients (author's transl)]. Postoperative results of lens implants after extracapsular cataract extraction in 99 of Dr. Binkhorst's patients in the year 1976/77. Evaluation of functional and morphological results. The visibility of the fundus is seriously compromised in comparison to intracapsular extracted aphakes without implant. Preoperative calculation of the lens to be implanted will reduce post-operative ammetropy to a minimum."}
{"id": "PMID:480842", "title": "[The implantation of intraocular lenses (author's transl)].", "content": "To become an implant surgeon means that one is ready to accept some opposition from part of the profession as well as to known all the shortcomings of conventional cataract extraction. A good knowledge of the history of implantation is necessary to avoid past errors in the shape, manufacture sterilization and in the locus of insertion of the intra-ocular lens. At the start few indications will be retained mainly unilateral cataracts traumatic or not, the aim being restoration of a full binocular vision. The interest of the test of Pulfrich is stressed for the follow up of cataract. A large number of contra-indications will be respected mainly corneal dystrophics or degeneration, uveitis, myopia, detachment and diabetes. The would-be implant surgeon has to train in modern extracapsular cataract extraction, learn the refinements of implantation technique with a full and constant respect of the corneal endothelium. He also should be able to cope with eventual complications and to suture the implant to the iris. He has at first to limit his choice to a small number of implant models which have proved safe: the iris clip and iridocapsular models of C. D. Binkhorst and the slotted-medallion of J. G. F. Worst. Experience will allow in time widening of indications and new improvements of technique.", "contents": "[The implantation of intraocular lenses (author's transl)]. To become an implant surgeon means that one is ready to accept some opposition from part of the profession as well as to known all the shortcomings of conventional cataract extraction. A good knowledge of the history of implantation is necessary to avoid past errors in the shape, manufacture sterilization and in the locus of insertion of the intra-ocular lens. At the start few indications will be retained mainly unilateral cataracts traumatic or not, the aim being restoration of a full binocular vision. The interest of the test of Pulfrich is stressed for the follow up of cataract. A large number of contra-indications will be respected mainly corneal dystrophics or degeneration, uveitis, myopia, detachment and diabetes. The would-be implant surgeon has to train in modern extracapsular cataract extraction, learn the refinements of implantation technique with a full and constant respect of the corneal endothelium. He also should be able to cope with eventual complications and to suture the implant to the iris. He has at first to limit his choice to a small number of implant models which have proved safe: the iris clip and iridocapsular models of C. D. Binkhorst and the slotted-medallion of J. G. F. Worst. Experience will allow in time widening of indications and new improvements of technique."}
{"id": "PMID:480843", "title": "[A review of 400 intraocular lens implants (author's transl)].", "content": "400 intraocular lenses (iris-clip and iridocapsular pseudophakoi of the Binkhorst type) were implanted at the Lucerne Eye Hospital between 1973 and 1978. The present review emphasizes the paramount position of the ophthalmosurgeon among the complicating factors. The 2-loop metallic iridocapsular lenses were abandonned; secured iris-clip lenses are now after intra- and extra-capsular lens extraction. Aspiration of lens masses or needling of a secundary cataract were necessary in 27% of extracapsular lens extraction. Precipitates on the psuedophakoi were absent after intrakapsular lens extraction, indicating the uveitis being of phacogenic, not pseudophacogenic origin.", "contents": "[A review of 400 intraocular lens implants (author's transl)]. 400 intraocular lenses (iris-clip and iridocapsular pseudophakoi of the Binkhorst type) were implanted at the Lucerne Eye Hospital between 1973 and 1978. The present review emphasizes the paramount position of the ophthalmosurgeon among the complicating factors. The 2-loop metallic iridocapsular lenses were abandonned; secured iris-clip lenses are now after intra- and extra-capsular lens extraction. Aspiration of lens masses or needling of a secundary cataract were necessary in 27% of extracapsular lens extraction. Precipitates on the psuedophakoi were absent after intrakapsular lens extraction, indicating the uveitis being of phacogenic, not pseudophacogenic origin."}
{"id": "PMID:480844", "title": "[Intraocular lenses - aphorisms of an operating practitioner (author's transl)].", "content": "The implantation of an intraocular lens is an almost ideal solution to manage monocular cataracts. We recommend this operation if medically indicated; it is technically more difficult, needs microsurgical skill and general anesthesia and therefore should be limited to a reduced number of highly qualified operation-teams. Uncontrolled enlargement of indications will result in higher complication-rates. As we do not yet know on complications due to alteration of foreign intraocular material after many years, we rather prefer to operate double-sided cataracts in the conventional way, specially in younger patients. Regarding to the global problems of curable (operable) blindness, we think that young eye-surgeons should be trained for conventional cataract operations without using microscope.", "contents": "[Intraocular lenses - aphorisms of an operating practitioner (author's transl)]. The implantation of an intraocular lens is an almost ideal solution to manage monocular cataracts. We recommend this operation if medically indicated; it is technically more difficult, needs microsurgical skill and general anesthesia and therefore should be limited to a reduced number of highly qualified operation-teams. Uncontrolled enlargement of indications will result in higher complication-rates. As we do not yet know on complications due to alteration of foreign intraocular material after many years, we rather prefer to operate double-sided cataracts in the conventional way, specially in younger patients. Regarding to the global problems of curable (operable) blindness, we think that young eye-surgeons should be trained for conventional cataract operations without using microscope."}
{"id": "PMID:480845", "title": "[Special indications for lens implantation (author's transl)].", "content": "After hesitation in the indication for the implantation of irisclip-lenses, experience shows that indications which have not yet been planned must be seriously considered: for instance cataract in patients with retinitis pigmentosa. The already highly restricted visual field is not additionally reduced by the wearing of the strong convex glasses. Furthermore the author reports about refinements of the implantation technique.", "contents": "[Special indications for lens implantation (author's transl)]. After hesitation in the indication for the implantation of irisclip-lenses, experience shows that indications which have not yet been planned must be seriously considered: for instance cataract in patients with retinitis pigmentosa. The already highly restricted visual field is not additionally reduced by the wearing of the strong convex glasses. Furthermore the author reports about refinements of the implantation technique."}
{"id": "PMID:480846", "title": "[Special indications, contraindications, particular methods of treatment in correction with intraocular lenses (author's transl)].", "content": "For the past 5 3/4 years intraocular lens implantion belongs to the routine operations performed in the Lucerne Eye Clinic. Whereas unilateral cataracts are the main and frequent indication for intraocular lens implants, there are certain cases of uni- and bilateral cataracts also, where the implantation of intraocular lens, due to its advantages, is specially indicated. Such cases are presented briefly in this paper. Good results are achieved through strictly observing the methods of treatment before and after the implantation of the lens.", "contents": "[Special indications, contraindications, particular methods of treatment in correction with intraocular lenses (author's transl)]. For the past 5 3/4 years intraocular lens implantion belongs to the routine operations performed in the Lucerne Eye Clinic. Whereas unilateral cataracts are the main and frequent indication for intraocular lens implants, there are certain cases of uni- and bilateral cataracts also, where the implantation of intraocular lens, due to its advantages, is specially indicated. Such cases are presented briefly in this paper. Good results are achieved through strictly observing the methods of treatment before and after the implantation of the lens."}
{"id": "PMID:480847", "title": "[Treatment of retinoblastoma (author's transl)].", "content": "Retinoblastoma remains a rare disease, but still one which has an uncertain life and functional prognosis. The diagnosis is often made late because the principal signs such as leukokoria and strabismus are unrecognized, thus a conservative treatment is not possible. However, treated at an early stage, a child suffering from retinoblastoma has every chance of a complete cure and even normal vision may be preserved. Irradiation therapy plays an essential role, using localized radiation by radioactive applicators or by external betatron electron-beams. Based on 52 cases, the methods of treatment and the results obtained at the Ophthalmology Department of Lausanne are reported, including one case of spontaneous cure of a bilateral retinoblastoma.", "contents": "[Treatment of retinoblastoma (author's transl)]. Retinoblastoma remains a rare disease, but still one which has an uncertain life and functional prognosis. The diagnosis is often made late because the principal signs such as leukokoria and strabismus are unrecognized, thus a conservative treatment is not possible. However, treated at an early stage, a child suffering from retinoblastoma has every chance of a complete cure and even normal vision may be preserved. Irradiation therapy plays an essential role, using localized radiation by radioactive applicators or by external betatron electron-beams. Based on 52 cases, the methods of treatment and the results obtained at the Ophthalmology Department of Lausanne are reported, including one case of spontaneous cure of a bilateral retinoblastoma."}
{"id": "PMID:480848", "title": "[The treatment of melanoma of the choroid using localized radiation of cobalt 60 applicators (author's transl)].", "content": "Sixty one cases of melanoma of the choroid have been treated conservatively in our Department over the last 9 years, using Cobalt 60 applicators. Analysis of these cases has shown that the tumours most susceptible to, and which react favourably to this treatment, are those of less than 11 mm diameter which are not in contact either with the macula or the optic nerve.", "contents": "[The treatment of melanoma of the choroid using localized radiation of cobalt 60 applicators (author's transl)]. Sixty one cases of melanoma of the choroid have been treated conservatively in our Department over the last 9 years, using Cobalt 60 applicators. Analysis of these cases has shown that the tumours most susceptible to, and which react favourably to this treatment, are those of less than 11 mm diameter which are not in contact either with the macula or the optic nerve."}
{"id": "PMID:480849", "title": "[The echography of ocular tumours and their microscopic architecture (author's transl)].", "content": "The echography of ocular tumours (7 malignant melanomas and 2 metastatic tumours) is presented in correlation with their microscopic architecture. A metastatic tumour presenting echographic characteristics typical of malignant melanoma was considered as such at the ultrasound test. All 7 malignant tumours were diagnosed through the ultrasound test, but it was impossible to foretell a predominant type of cell.", "contents": "[The echography of ocular tumours and their microscopic architecture (author's transl)]. The echography of ocular tumours (7 malignant melanomas and 2 metastatic tumours) is presented in correlation with their microscopic architecture. A metastatic tumour presenting echographic characteristics typical of malignant melanoma was considered as such at the ultrasound test. All 7 malignant tumours were diagnosed through the ultrasound test, but it was impossible to foretell a predominant type of cell."}
{"id": "PMID:480850", "title": "[Radiotherapy of intraocular tumours (author's transl)].", "content": "Ocular radiotherapy has considerably improved during the last 60 years. It allows very often, not only to save an eye, but also to maintain a useful function. But it is only one single element, although important, in our fight against intraocular tumors.", "contents": "[Radiotherapy of intraocular tumours (author's transl)]. Ocular radiotherapy has considerably improved during the last 60 years. It allows very often, not only to save an eye, but also to maintain a useful function. But it is only one single element, although important, in our fight against intraocular tumors."}
{"id": "PMID:480851", "title": "[Radiotherapy of intraocular tumours, particularly of melanoma of the choroid (author's transl)].", "content": "From 1964 to 1976 a number of 131 patients, suffering from melanoma of the choroid have been treated with 106Ru/106Rh beta-ray applicators. In 81 cases (61.8%) this treatment has been successful. 26 eyes (19.9%) had to be enucleated in spite of the irradiation. 24 patients (18.3%) died, 13 of them of metastases. Only in 46 patients, out of 81, we have reached total destruction of the tumor with flat chorioatrophic scar. In 27 cases visual acuity of 1.5 to 0.5 could be preserved. Radiogenic late complications in the capillary system with disturbances of the retinal blood circulation were the cause of visual deterioration. The 107 surviving patients were controlled during a period of 6.5 years in the average. Survival rate 91.2% after 5 and 84% after 10 years. Another group of 214 patients with melanoma of the choroid, who had been treated from 1955 to 1970 by enucleation reached a survival rate of 72% after 5 years. Treatment with 106Rh beta-irradiation therefore leads to no increased danger of metastases. The following indications for this treatment are suggested: 1. Prominence of the tumor not exceeding 5 mm, largest diameter at its base not more than 15 mm. 2. Distance of the dorsal edge of the tumor at least 1-2 optic disc diameters from the nerve head. 3. Peripheral delimitation against the ciliary body. 4. No tumor growth outside the eye.", "contents": "[Radiotherapy of intraocular tumours, particularly of melanoma of the choroid (author's transl)]. From 1964 to 1976 a number of 131 patients, suffering from melanoma of the choroid have been treated with 106Ru/106Rh beta-ray applicators. In 81 cases (61.8%) this treatment has been successful. 26 eyes (19.9%) had to be enucleated in spite of the irradiation. 24 patients (18.3%) died, 13 of them of metastases. Only in 46 patients, out of 81, we have reached total destruction of the tumor with flat chorioatrophic scar. In 27 cases visual acuity of 1.5 to 0.5 could be preserved. Radiogenic late complications in the capillary system with disturbances of the retinal blood circulation were the cause of visual deterioration. The 107 surviving patients were controlled during a period of 6.5 years in the average. Survival rate 91.2% after 5 and 84% after 10 years. Another group of 214 patients with melanoma of the choroid, who had been treated from 1955 to 1970 by enucleation reached a survival rate of 72% after 5 years. Treatment with 106Rh beta-irradiation therefore leads to no increased danger of metastases. The following indications for this treatment are suggested: 1. Prominence of the tumor not exceeding 5 mm, largest diameter at its base not more than 15 mm. 2. Distance of the dorsal edge of the tumor at least 1-2 optic disc diameters from the nerve head. 3. Peripheral delimitation against the ciliary body. 4. No tumor growth outside the eye."}
{"id": "PMID:480853", "title": "[Complications of the irradiation therapy with beta applicators in malign melanomas (author's transl)].", "content": "Depending on prominence and localization of malign melanomas of the choroid, there are - besides enucleation - other clinical treatments possible. Irradiation therapy with beta applicators (106Ru/106Rh) gives also radiogenous complications. But contrarily to a treatment with cobalt-60 applicators, these complications are less important.", "contents": "[Complications of the irradiation therapy with beta applicators in malign melanomas (author's transl)]. Depending on prominence and localization of malign melanomas of the choroid, there are - besides enucleation - other clinical treatments possible. Irradiation therapy with beta applicators (106Ru/106Rh) gives also radiogenous complications. But contrarily to a treatment with cobalt-60 applicators, these complications are less important."}
{"id": "PMID:480855", "title": "[Problems of surgical therapy after irradiation for tumors of the eyelids (author's transl)].", "content": "We discuss 10 consecutive cases which have had surgery after irradiation for tumors of the eyelids. Reasons for surgical treatment after irradiation: 1. Relaps within the irradiated area. Conditions of tissue prohibit additional irradiation. 2. No effect of irradiation. 3. Relaps in deeper layers or further growth, not noticed during irradiation. Additional irradiation ruinous for the eye. - Surgical problems after irradiation: 1. Inadequacy of usual techniques. 2. More extensive surgery then primary excision of tumor.", "contents": "[Problems of surgical therapy after irradiation for tumors of the eyelids (author's transl)]. We discuss 10 consecutive cases which have had surgery after irradiation for tumors of the eyelids. Reasons for surgical treatment after irradiation: 1. Relaps within the irradiated area. Conditions of tissue prohibit additional irradiation. 2. No effect of irradiation. 3. Relaps in deeper layers or further growth, not noticed during irradiation. Additional irradiation ruinous for the eye. - Surgical problems after irradiation: 1. Inadequacy of usual techniques. 2. More extensive surgery then primary excision of tumor."}
{"id": "PMID:480856", "title": "[Results of the 90Sr- and 90Y-radiation after surgical removal of 123 pterygia (author's transl)].", "content": "Out of 107 patients 123 pterygia were surgically removed: 24 pterygia of the progressive Type I, 49 of the semi progressive Type II and 50 of the regressive Type III. After surgical removal of the pterygia Type I and Type II followed a treatment with strontium 90 beta radiation with 2700 rep: 900 rep at the 2nd, 8th and 15th day after surgery. The recurrence rate was high in Type I (62%) and Type II (16%), but lower than it is reported in these groups operated without following radiation. More often pterygia of Type I and II are seen in people of the highlands.", "contents": "[Results of the 90Sr- and 90Y-radiation after surgical removal of 123 pterygia (author's transl)]. Out of 107 patients 123 pterygia were surgically removed: 24 pterygia of the progressive Type I, 49 of the semi progressive Type II and 50 of the regressive Type III. After surgical removal of the pterygia Type I and Type II followed a treatment with strontium 90 beta radiation with 2700 rep: 900 rep at the 2nd, 8th and 15th day after surgery. The recurrence rate was high in Type I (62%) and Type II (16%), but lower than it is reported in these groups operated without following radiation. More often pterygia of Type I and II are seen in people of the highlands."}
{"id": "PMID:480857", "title": "[Biochemical research in depression (author's transl)].", "content": "The biochemical research of depression did not gain in before the exploration of the nodes of effect of the antidepressants. For the present the point of research was the search for disturbances in metabolism of the biogenic amines in brain. The noradrenalin and serotonin-hypothesis was propounded postulating a disturbance in noradrenalin, or serotonin regulation, respectively at the receptor in depression. Until now experimental results did not support this hypothesis, just as the investigations of electrolytic changes in depression did not lead to homogeneous results. On the contrary the neuroendocrinological research showed important results; In endogenous depressive patients an increased cortisol-secretion was ascertained, and in about 65% of the patients a missing or strongly reduced cortisol-suppression after injection of dexamethason was noted, moreover, the growth-hormone-secretion after insulin-hypoglycemia is reduced in a part of depressive women in the menopause. Finally the thyrotropin-secretion stopped in 20--40% of the endogenous depressive patients after injection of thyrotropin-releasing hormone.", "contents": "[Biochemical research in depression (author's transl)]. The biochemical research of depression did not gain in before the exploration of the nodes of effect of the antidepressants. For the present the point of research was the search for disturbances in metabolism of the biogenic amines in brain. The noradrenalin and serotonin-hypothesis was propounded postulating a disturbance in noradrenalin, or serotonin regulation, respectively at the receptor in depression. Until now experimental results did not support this hypothesis, just as the investigations of electrolytic changes in depression did not lead to homogeneous results. On the contrary the neuroendocrinological research showed important results; In endogenous depressive patients an increased cortisol-secretion was ascertained, and in about 65% of the patients a missing or strongly reduced cortisol-suppression after injection of dexamethason was noted, moreover, the growth-hormone-secretion after insulin-hypoglycemia is reduced in a part of depressive women in the menopause. Finally the thyrotropin-secretion stopped in 20--40% of the endogenous depressive patients after injection of thyrotropin-releasing hormone."}
{"id": "PMID:480896", "title": "An isolator for avian disease research.", "content": "An isolator is described, fabricated in glass-reinforced plastic, suitable for housing 25-30 chicks to 6 weeks of age, or for fewer older birds, for use in experiments involving infectious agents.", "contents": "An isolator for avian disease research. An isolator is described, fabricated in glass-reinforced plastic, suitable for housing 25-30 chicks to 6 weeks of age, or for fewer older birds, for use in experiments involving infectious agents."}
{"id": "PMID:480898", "title": "Infiltrating pituitary neoplasms in the rat.", "content": "11 cases of spontaneous pituitary neoplasms in rats, which showed infiltration into brain tissue, are reported. This represents an incidence of about 0.42%.", "contents": "Infiltrating pituitary neoplasms in the rat. 11 cases of spontaneous pituitary neoplasms in rats, which showed infiltration into brain tissue, are reported. This represents an incidence of about 0.42%."}
{"id": "PMID:480899", "title": "The ferret, Mustela putorius furo, as a new species in toxicology.", "content": "Comprehensive studies on the haematology, urine chemistry, serum chemistry and gross pathology have been carried out and reported. Seasonal weight changes were confirmed and were initiated, together with sexual activity, when ferrets were brought in to a constant temperature (18-19 degrees C) and 14-hour daylight cycle during the winter months. Levels of haemoglobin, erythrocytes and haematocrit were higher than in most common laboratory species. Similarly, serum electrolytes and glucose levels were higher than in Wistar rats or beagle dogs. Ovaries, uteri and testes displayed a seasonal maturation and atrophy. All other parameters were similar to common laboratory species.", "contents": "The ferret, Mustela putorius furo, as a new species in toxicology. Comprehensive studies on the haematology, urine chemistry, serum chemistry and gross pathology have been carried out and reported. Seasonal weight changes were confirmed and were initiated, together with sexual activity, when ferrets were brought in to a constant temperature (18-19 degrees C) and 14-hour daylight cycle during the winter months. Levels of haemoglobin, erythrocytes and haematocrit were higher than in most common laboratory species. Similarly, serum electrolytes and glucose levels were higher than in Wistar rats or beagle dogs. Ovaries, uteri and testes displayed a seasonal maturation and atrophy. All other parameters were similar to common laboratory species."}
{"id": "PMID:480900", "title": "Financial control of laboratory animal facilities: partial cost recovery in an academic institution.", "content": "The objective of cost management in the animal house is to make efficient use of resources and to regulate the activity of the user. Regular charging of users keeps them aware of their responsibility both ethically and financially. Forward budgetary control is encouraged and regulation of the work load achieved through planned requirements helps to reduce animal wastage.", "contents": "Financial control of laboratory animal facilities: partial cost recovery in an academic institution. The objective of cost management in the animal house is to make efficient use of resources and to regulate the activity of the user. Regular charging of users keeps them aware of their responsibility both ethically and financially. Forward budgetary control is encouraged and regulation of the work load achieved through planned requirements helps to reduce animal wastage."}
{"id": "PMID:480901", "title": "Vaginal opening in the Mongolian gerbil, Meriones unguiculatus: normal data and the influence of social factors.", "content": "Mean age at vaginal opening was 41 days, range 33-53 days. Bodyweight at weaning and age at vaginal opening were negatively correlated, but there was no relationship between bodyweight and age at vaginal opening. The presence of a male before vaginal opening did not accelerate either the time of its occurrence or first conception. The vaginas of females caged singly from weaning opened earlier than in those kept in groups of 2 or 3.", "contents": "Vaginal opening in the Mongolian gerbil, Meriones unguiculatus: normal data and the influence of social factors. Mean age at vaginal opening was 41 days, range 33-53 days. Bodyweight at weaning and age at vaginal opening were negatively correlated, but there was no relationship between bodyweight and age at vaginal opening. The presence of a male before vaginal opening did not accelerate either the time of its occurrence or first conception. The vaginas of females caged singly from weaning opened earlier than in those kept in groups of 2 or 3."}
{"id": "PMID:480902", "title": "Transfer isolator to protect personnel from exposure to peracetic acid.", "content": "A transfer isolator is described which confines peracetic acid fumes used in the gnotobiotic operation of isolators. The use of this isolator protects both personnel and animals from contact  with peracetic acid, provides additional isolator space and reduces wear on the gloves and sleeves of the main isolator.", "contents": "Transfer isolator to protect personnel from exposure to peracetic acid. A transfer isolator is described which confines peracetic acid fumes used in the gnotobiotic operation of isolators. The use of this isolator protects both personnel and animals from contact  with peracetic acid, provides additional isolator space and reduces wear on the gloves and sleeves of the main isolator."}
{"id": "PMID:480903", "title": "Mating post partum, concurrent lactation and reproduction in the laboratory rat.", "content": "32 of 35 sexually mature CFHB rats paired with proven males and thereafter allowed to mate post partum and nurse young concurrent with pregnancy, produced a total of 227 litters. The proportion of females producing litters decreased both with increasing age and parity, and with the number of young nursed. Litter size declined both as age and parity increased, and when gestation was extremely prolonged. The incidence and extent of delayed implantation (gestations greater than 25 days) were affected by the number of young nursed but not by increasing age and parity. There was no evidence for the existence of implantation periods coinciding with multiples of oestrous cycles.", "contents": "Mating post partum, concurrent lactation and reproduction in the laboratory rat. 32 of 35 sexually mature CFHB rats paired with proven males and thereafter allowed to mate post partum and nurse young concurrent with pregnancy, produced a total of 227 litters. The proportion of females producing litters decreased both with increasing age and parity, and with the number of young nursed. Litter size declined both as age and parity increased, and when gestation was extremely prolonged. The incidence and extent of delayed implantation (gestations greater than 25 days) were affected by the number of young nursed but not by increasing age and parity. There was no evidence for the existence of implantation periods coinciding with multiples of oestrous cycles."}
{"id": "PMID:480904", "title": "Chronic non-infective conjunctivitis in rabbits.", "content": "A high proportion (36%) of rabbits in a long-term experiment developed a severe chronic purulent conjunctivitis. Bacteriological examination failed to reveal an organism common to all cases, and the condition was only partially controlled by a neomycin and hydrocortisone eye ointment. Cutting down the possibility of hay dust entering the rabbits' eyes led to marked improvement: the conjunctivitis was virtually eliminated when hay was given in a specially-designed solid-sided hopper which prevented the release of dust during feeding and which, being detachable, could be refilled away from the rabbit rooms to minimize general atmospheric dust.", "contents": "Chronic non-infective conjunctivitis in rabbits. A high proportion (36%) of rabbits in a long-term experiment developed a severe chronic purulent conjunctivitis. Bacteriological examination failed to reveal an organism common to all cases, and the condition was only partially controlled by a neomycin and hydrocortisone eye ointment. Cutting down the possibility of hay dust entering the rabbits' eyes led to marked improvement: the conjunctivitis was virtually eliminated when hay was given in a specially-designed solid-sided hopper which prevented the release of dust during feeding and which, being detachable, could be refilled away from the rabbit rooms to minimize general atmospheric dust."}
{"id": "PMID:480905", "title": "Genic heterogeneity and genetic monitoring of mouse outbred stocks.", "content": "An outbred stock of Swiss:SE mice has been surveyed for genic heterogeneity at 12 loci, encoding biochemical polymorphisms in mice. Using horizontal starch-gel electrophoresis, 6 loci (Es-1, Es-2, Es-3, Es-5, Trf, Dip-1) revealed no genic heterogeneity within the total sample of 289 male mice examined. The other 6 loci (Mpi-1, Mup-1, Hbb, Gpi-1, Pgm-1, Ldr-1) each showed a 2-allelic variation within each of the 3 stock units (pavillions) involved. A pavillion effect on the observed genotype numbers was found for Pgm-1 and Gpi-1 (P less than 0.10). Inadequate genotyping may have occurred at Gpi-1 and Ldr-1, as suggested by a 'week effect' on the observed genotype numbers (P less than 0.05). For studying long-term genetic changes within outbred stocks, a routine monitoring procedure using biochemical polymorphisms is recommended.", "contents": "Genic heterogeneity and genetic monitoring of mouse outbred stocks. An outbred stock of Swiss:SE mice has been surveyed for genic heterogeneity at 12 loci, encoding biochemical polymorphisms in mice. Using horizontal starch-gel electrophoresis, 6 loci (Es-1, Es-2, Es-3, Es-5, Trf, Dip-1) revealed no genic heterogeneity within the total sample of 289 male mice examined. The other 6 loci (Mpi-1, Mup-1, Hbb, Gpi-1, Pgm-1, Ldr-1) each showed a 2-allelic variation within each of the 3 stock units (pavillions) involved. A pavillion effect on the observed genotype numbers was found for Pgm-1 and Gpi-1 (P less than 0.10). Inadequate genotyping may have occurred at Gpi-1 and Ldr-1, as suggested by a 'week effect' on the observed genotype numbers (P less than 0.05). For studying long-term genetic changes within outbred stocks, a routine monitoring procedure using biochemical polymorphisms is recommended."}
{"id": "PMID:480906", "title": "Early postnatal physical and behavioural development of degus (Octodon degus).", "content": "6 litters of Octodon degus were studied from birth to 10 days of age. Newly-born degus (mean weight 14.6 g) had open eyes, upper and lower pigment, fur, and teeth. Within 3-4 hours of birth they were able to walk supporting their full weight, right themselves rapidly, sit upright on their haunches, or rear upright with support, and vocalize. By the 1st or 2nd day, the young animals displayed functional grooming (face washing, hind-paw scratching, rapid head-shake). Solid food was ingested from day 6, although newborns chewed wood chips and 3-day olds gnawed dried faeces. Young degus were tested daily in an open field apparatus and showed increased activity and exploration, with repeated testing while decreasing distress vocalization after the 4th or 5th day. Degus are proposed for the study of developmental topics since their degree of development at birth allows for immediate testing. The degus studied here seem to be more fully developed at birth than those studied in Britain.", "contents": "Early postnatal physical and behavioural development of degus (Octodon degus). 6 litters of Octodon degus were studied from birth to 10 days of age. Newly-born degus (mean weight 14.6 g) had open eyes, upper and lower pigment, fur, and teeth. Within 3-4 hours of birth they were able to walk supporting their full weight, right themselves rapidly, sit upright on their haunches, or rear upright with support, and vocalize. By the 1st or 2nd day, the young animals displayed functional grooming (face washing, hind-paw scratching, rapid head-shake). Solid food was ingested from day 6, although newborns chewed wood chips and 3-day olds gnawed dried faeces. Young degus were tested daily in an open field apparatus and showed increased activity and exploration, with repeated testing while decreasing distress vocalization after the 4th or 5th day. Degus are proposed for the study of developmental topics since their degree of development at birth allows for immediate testing. The degus studied here seem to be more fully developed at birth than those studied in Britain."}
{"id": "PMID:480908", "title": "Hepatitis B surface antigen (HBsAg) in leprosy patients of Calcutta: its prevalence and subtypes.", "content": "Hight incidence of hepatitis B surface antigen (HBsAg) in lepromatous leprosy patients have been reported by many workers. This paper reports on a study undertaken over a period of more than two years to determine the prevalence of HBsAg and its major antigenic subtypes amongst the various clinical types of leprosy and apparently healthy individuals of Calcutta population. The overall incidence of HBsAg in lepromatous leprosy is found to be 3.8% (9 out of 234) in our study. The incidence of HBsAg in tuberculoid leprosy and non leprosy control cases were almost identical, 2.5% (11 out of 431) and 2.7% (14 out of 519) respectively. The incidence of HBsAg in lepromatous leprosy in the present study was apparently higher than tuberculoid or non leprosy cases, although statistically not significant. Hence the association between HBsAg and lepromatous leprosy could not be established in our study. None of the major antigenic subtypes of HBsAg could be related to any particular type of leprosy. The subtypes 'ad', 'ay' and 'ar' were present at varying proportions in all the groups tested. The antigenic subtype 'ay' was found to be prevalent in Calcutta population in higher proportions.", "contents": "Hepatitis B surface antigen (HBsAg) in leprosy patients of Calcutta: its prevalence and subtypes. Hight incidence of hepatitis B surface antigen (HBsAg) in lepromatous leprosy patients have been reported by many workers. This paper reports on a study undertaken over a period of more than two years to determine the prevalence of HBsAg and its major antigenic subtypes amongst the various clinical types of leprosy and apparently healthy individuals of Calcutta population. The overall incidence of HBsAg in lepromatous leprosy is found to be 3.8% (9 out of 234) in our study. The incidence of HBsAg in tuberculoid leprosy and non leprosy control cases were almost identical, 2.5% (11 out of 431) and 2.7% (14 out of 519) respectively. The incidence of HBsAg in lepromatous leprosy in the present study was apparently higher than tuberculoid or non leprosy cases, although statistically not significant. Hence the association between HBsAg and lepromatous leprosy could not be established in our study. None of the major antigenic subtypes of HBsAg could be related to any particular type of leprosy. The subtypes 'ad', 'ay' and 'ar' were present at varying proportions in all the groups tested. The antigenic subtype 'ay' was found to be prevalent in Calcutta population in higher proportions."}
{"id": "PMID:480909", "title": "Serum immunoglobulins in leprosy.", "content": "Serum immunoglobulins were quantitated by radial immunodiffusion in 25 cases each of tuberculoid and lepromatous leprosy. Immunoglobulins estimated from 50 normal healthy adults were the control. Serum IgG was markedly raised in both tuberculoid (mean 2420 mg/dl) and lepromatous leprosy (mean 2493 mg/dl) when compared with the controls (mean 1288 mg/dl) and the difference was significant (p less than 0.01). However the difference in serum IgM and IgA levels in cases as compared to controls were not statistically significant. Serum IgM was slightly raised, the mean values obtained being 222 mg/dl in tuberculoid leprosy, 221 mg/dl in lepromatous leprosy and 202 mg/dl in control. Serum IgA was reduced in lepromatous leprosy (mean 129 mg/dl) as compared to the controls (mean 168 mg/dl) and the cases of tuberculoid leprosy (mean 165 mg/dl). The range of values obtained in both groups of patients showed greater scatter than the controls and a few cases of both forms of leprosy showed very low values of both serum IgA and IgM.", "contents": "Serum immunoglobulins in leprosy. Serum immunoglobulins were quantitated by radial immunodiffusion in 25 cases each of tuberculoid and lepromatous leprosy. Immunoglobulins estimated from 50 normal healthy adults were the control. Serum IgG was markedly raised in both tuberculoid (mean 2420 mg/dl) and lepromatous leprosy (mean 2493 mg/dl) when compared with the controls (mean 1288 mg/dl) and the difference was significant (p less than 0.01). However the difference in serum IgM and IgA levels in cases as compared to controls were not statistically significant. Serum IgM was slightly raised, the mean values obtained being 222 mg/dl in tuberculoid leprosy, 221 mg/dl in lepromatous leprosy and 202 mg/dl in control. Serum IgA was reduced in lepromatous leprosy (mean 129 mg/dl) as compared to the controls (mean 168 mg/dl) and the cases of tuberculoid leprosy (mean 165 mg/dl). The range of values obtained in both groups of patients showed greater scatter than the controls and a few cases of both forms of leprosy showed very low values of both serum IgA and IgM."}
{"id": "PMID:480910", "title": "A study of serum fibrinolytic activity in leprosy.", "content": "Fibrinolytic activity was studied in 50 patients of leprosy and 30 healthy individuals who served as control. Fibrinolytic activity was determined by measuring euglobulin lysis. No significant alteration in fibrinolytic activity was observed in patients with non-lepromatous leprosy, the levels being approximately similar to control group. However, fibrinolytic activity was found to be significantly decreased in patients of lepromatous leprosy and lepra reaction group. The observed decrease in fibrinolytic activity can be explained on the basis of presence of tissue destruction and vasculitis seen in leprosy more so in patients with lepromatous leprosy and lepra reaction.", "contents": "A study of serum fibrinolytic activity in leprosy. Fibrinolytic activity was studied in 50 patients of leprosy and 30 healthy individuals who served as control. Fibrinolytic activity was determined by measuring euglobulin lysis. No significant alteration in fibrinolytic activity was observed in patients with non-lepromatous leprosy, the levels being approximately similar to control group. However, fibrinolytic activity was found to be significantly decreased in patients of lepromatous leprosy and lepra reaction group. The observed decrease in fibrinolytic activity can be explained on the basis of presence of tissue destruction and vasculitis seen in leprosy more so in patients with lepromatous leprosy and lepra reaction."}
{"id": "PMID:480911", "title": "A study of patients with erythema nondosum leprosum syndrome.", "content": "25 cases of Erythema Nodosum Leprosum (ENL) Syndrome have been clinically evaluated. Majority patients (84%) were males in the middle age group. Fever (56%), arthralgia (100%) and neuritic pains (100%) were common presenting constitutional symptoms. ENL was not related to DDS therapy or to any precipitating factors. Severity of reaction graded by clinical scoring was well correlated with fibrinolytic activity. Fibrinolytic activity was found decreased in all the cases. The decreases in fibrinolytic activity was more so pronounced in patients having higher clinical scorings.", "contents": "A study of patients with erythema nondosum leprosum syndrome. 25 cases of Erythema Nodosum Leprosum (ENL) Syndrome have been clinically evaluated. Majority patients (84%) were males in the middle age group. Fever (56%), arthralgia (100%) and neuritic pains (100%) were common presenting constitutional symptoms. ENL was not related to DDS therapy or to any precipitating factors. Severity of reaction graded by clinical scoring was well correlated with fibrinolytic activity. Fibrinolytic activity was found decreased in all the cases. The decreases in fibrinolytic activity was more so pronounced in patients having higher clinical scorings."}
{"id": "PMID:480912", "title": "Reconstructive surgery of claw hand in leprosy.", "content": "32 hands in 31 known cases of leprosy were operated using the Paul-Brand technique for the intrinsic minus deformity. 29 hands revealed good results whereas remaining 3 also had definite improvement in their function and cosmesis. Bunnel's opponens plasty was performed in 7 cases to restore back the power of opponens in the thumb. Meticulous pre and postoperative management proved to be very necessary.", "contents": "Reconstructive surgery of claw hand in leprosy. 32 hands in 31 known cases of leprosy were operated using the Paul-Brand technique for the intrinsic minus deformity. 29 hands revealed good results whereas remaining 3 also had definite improvement in their function and cosmesis. Bunnel's opponens plasty was performed in 7 cases to restore back the power of opponens in the thumb. Meticulous pre and postoperative management proved to be very necessary."}
{"id": "PMID:480913", "title": "Dapsone treatment and deformities. A retrospective study.", "content": "Gandhi Memorial Leprosy Foundation conducted a study from 1963-72. Records of 2608 patients pertaining to type of leprosy, year of detection and registration, involvement of nerves, treatment, reactions, deformities, were available for a retrospective study. Patients having problems like neuritis and deformities and of the types lepromatous and borderline were the ones who tended to be regular clinic attenders. Lepromatous and Borderline and Polyneuritic types and N3 group were prone to develop deformities. There seemed to be association between reaction and causation of deformities. Because of the neurotoxic effect and ability to concentrate in the affected nerves, dapsone might enhance the risk of deformities. Low deformity rates in N1 and N2 types of nerve involvement reaffirmed the necessity of early diagnosis of leprosy.", "contents": "Dapsone treatment and deformities. A retrospective study. Gandhi Memorial Leprosy Foundation conducted a study from 1963-72. Records of 2608 patients pertaining to type of leprosy, year of detection and registration, involvement of nerves, treatment, reactions, deformities, were available for a retrospective study. Patients having problems like neuritis and deformities and of the types lepromatous and borderline were the ones who tended to be regular clinic attenders. Lepromatous and Borderline and Polyneuritic types and N3 group were prone to develop deformities. There seemed to be association between reaction and causation of deformities. Because of the neurotoxic effect and ability to concentrate in the affected nerves, dapsone might enhance the risk of deformities. Low deformity rates in N1 and N2 types of nerve involvement reaffirmed the necessity of early diagnosis of leprosy."}
{"id": "PMID:480914", "title": "Screening for diabetes mellitus in leprosy patients with complicated ulcers.", "content": "All patients admitted to an ulcer ward in a leprosy hospital over the period of one year were screened for glycosuria. Out of 154 patients screened 4 showed glycosuria. These 4 patients had more severely complicated ulcers evidenced by their longer duration of admission. Early diagnosis and treatment of diabetes in leprosy patients with complicated ulcers is important.", "contents": "Screening for diabetes mellitus in leprosy patients with complicated ulcers. All patients admitted to an ulcer ward in a leprosy hospital over the period of one year were screened for glycosuria. Out of 154 patients screened 4 showed glycosuria. These 4 patients had more severely complicated ulcers evidenced by their longer duration of admission. Early diagnosis and treatment of diabetes in leprosy patients with complicated ulcers is important."}
{"id": "PMID:480915", "title": "Medical records in leprosy programme.", "content": "It is an established fact that Medical Records play a vital role in the care of various diseases but it has a very important and significant role in a disease like leprosy. Its importance should not be underestimated as Leprosy poses many problems which have some unique characteristics and special features. First of all it is to be accepted that our knowledge of Leprosy is still none to no where, in spite of the age old nature of the disease it is imperative to have proper records of the disease which may lead to invaluable results.", "contents": "Medical records in leprosy programme. It is an established fact that Medical Records play a vital role in the care of various diseases but it has a very important and significant role in a disease like leprosy. Its importance should not be underestimated as Leprosy poses many problems which have some unique characteristics and special features. First of all it is to be accepted that our knowledge of Leprosy is still none to no where, in spite of the age old nature of the disease it is imperative to have proper records of the disease which may lead to invaluable results."}
{"id": "PMID:480916", "title": "Acute fatal DDS poisoning. (Report of 4 cases).", "content": "Acute dapsone poisoning is rare and such cases are either accidental or suicidal. Though accidental DDS poisoning are reported in children, the same is fairly uncommon in adults. Only 2 such cases are reported in India literature. We here report 4 cases of fatal sucidial DDS poisoning in adults resulting death in 3 cases. The reported acute symptoms include nausea, vomiting, hyperexcitability followed by depression, Carpopedal spasm or convulsions. The most marked signs are dyspnoea and cyanosis. The symptoms are due to methaemoglobinaemia, and or sulphaemoglobinaemia. Normally dapsone induces red cell haemolysis and even with small therapeutic doses of 25-100 mg per day, and in toxic doses reduces the oxygen carrying capacity of blood and damages the red cells making them more vulnerable for haemolysis. The peculiarity of the presentation in this series are manifestation of severe haemorrhagic episode in one case and progressive jaundice in another besides cyanosis. None of the cases had carpopedal spasm or convulsion. Out of four cases three died inspite of intensive care, intravenous vitamin C, exchange transfusion (2 cases) and other supportive measures. Intravenous methylene blue could not be used in these cases due to non-availability.", "contents": "Acute fatal DDS poisoning. (Report of 4 cases). Acute dapsone poisoning is rare and such cases are either accidental or suicidal. Though accidental DDS poisoning are reported in children, the same is fairly uncommon in adults. Only 2 such cases are reported in India literature. We here report 4 cases of fatal sucidial DDS poisoning in adults resulting death in 3 cases. The reported acute symptoms include nausea, vomiting, hyperexcitability followed by depression, Carpopedal spasm or convulsions. The most marked signs are dyspnoea and cyanosis. The symptoms are due to methaemoglobinaemia, and or sulphaemoglobinaemia. Normally dapsone induces red cell haemolysis and even with small therapeutic doses of 25-100 mg per day, and in toxic doses reduces the oxygen carrying capacity of blood and damages the red cells making them more vulnerable for haemolysis. The peculiarity of the presentation in this series are manifestation of severe haemorrhagic episode in one case and progressive jaundice in another besides cyanosis. None of the cases had carpopedal spasm or convulsion. Out of four cases three died inspite of intensive care, intravenous vitamin C, exchange transfusion (2 cases) and other supportive measures. Intravenous methylene blue could not be used in these cases due to non-availability."}
{"id": "PMID:480917", "title": "Erythema multiforme bullosum due to rifampicin.", "content": "A case of Erythema Multiforme Bullosum in patient of lepromatous leprosy with pulmonary tuberculosis due to Rifampicin is described. It is stressed that ethambutol may act as a trigger factor to the toxic effects of Rifampicin.", "contents": "Erythema multiforme bullosum due to rifampicin. A case of Erythema Multiforme Bullosum in patient of lepromatous leprosy with pulmonary tuberculosis due to Rifampicin is described. It is stressed that ethambutol may act as a trigger factor to the toxic effects of Rifampicin."}
{"id": "PMID:480932", "title": "Speech perception by profoundly hearing-impaired children.", "content": "This article reviews the limitations of defining profound hearing impairment solely by reference to pure-tone audiograms. Instead, profoundly hearing-impaired children may be described as those whose ability to distinguish spectral features in speech is extremely poor, and for whom the gross intensity variations in the waveform envelope are the principal acoustic cues. Examples of pattern cues that are available to profoundly hearing-imparied children are presented through an optical analog, and the usefulness of these cues is shown with regard to vowel and consonant identification, word stress-pattern perception, and distinction among sentences that differ in number of syllables, pattern, intensity, or rate. Perception of speech material through vision (lipreading) and also through combined auditory and visual modes are described, with a discussion of articulatory and language factors that influence intelligibility. Instructional strategies are briefly reviewed as they relate to educational improvement of speech-perception abilities of profoundly hearing-impaired children and to the development of special sensory aids.", "contents": "Speech perception by profoundly hearing-impaired children. This article reviews the limitations of defining profound hearing impairment solely by reference to pure-tone audiograms. Instead, profoundly hearing-impaired children may be described as those whose ability to distinguish spectral features in speech is extremely poor, and for whom the gross intensity variations in the waveform envelope are the principal acoustic cues. Examples of pattern cues that are available to profoundly hearing-imparied children are presented through an optical analog, and the usefulness of these cues is shown with regard to vowel and consonant identification, word stress-pattern perception, and distinction among sentences that differ in number of syllables, pattern, intensity, or rate. Perception of speech material through vision (lipreading) and also through combined auditory and visual modes are described, with a discussion of articulatory and language factors that influence intelligibility. Instructional strategies are briefly reviewed as they relate to educational improvement of speech-perception abilities of profoundly hearing-impaired children and to the development of special sensory aids."}
{"id": "PMID:480933", "title": "Speech and language testing with distinct ethnic-racial groups: a survey of procedures for improving validity.", "content": "Several procedures are being used currently to improve the validity of speech and language evaluation for ethnic or racial groups from lower-income populations. Procedures mentioned in this article include: (1) developing a new test, (2) modifying test items or keyed responses on existing tests, and (3) developing new norms for existing tests. Examples are given for each procedure, and advantages and disadvantages are discussed.", "contents": "Speech and language testing with distinct ethnic-racial groups: a survey of procedures for improving validity. Several procedures are being used currently to improve the validity of speech and language evaluation for ethnic or racial groups from lower-income populations. Procedures mentioned in this article include: (1) developing a new test, (2) modifying test items or keyed responses on existing tests, and (3) developing new norms for existing tests. Examples are given for each procedure, and advantages and disadvantages are discussed."}
{"id": "PMID:480934", "title": "The influence of pauses in the competing signal on synthetic sentence identification scores.", "content": "Thirty normal-hearing subjects participated in an experiment testing the effects of pauses in the competing discourse on the synthetic sentence identification (SSI) task. When speech noise was added to the continuous discourse used for competition, subjects found it more difficult to identify the key words in the synthetic sentences.", "contents": "The influence of pauses in the competing signal on synthetic sentence identification scores. Thirty normal-hearing subjects participated in an experiment testing the effects of pauses in the competing discourse on the synthetic sentence identification (SSI) task. When speech noise was added to the continuous discourse used for competition, subjects found it more difficult to identify the key words in the synthetic sentences."}
{"id": "PMID:480935", "title": "Speech, language and hearing disorders in a adult penal institution.", "content": "It has been speculated that the prevalence of communicative disorders among prison inmates is considerably higher than that found in the general population, but research regarding inmate speech and hearing disorders is limited. This study investigated the nature and extent of communicative disorders in an inmate population of a medium-security penal institution in southeastern Virginia. The results of the screening indicated a slightly lower prevalence of stuttering, higher prevalences of articulation, voice, and hearing disorders, and more deficient receptive vocabulary skills than found in the general population. Some dialectal variations among black inmates are noted and the possible influence of linguistic-cultural interference on the results is discussed.", "contents": "Speech, language and hearing disorders in a adult penal institution. It has been speculated that the prevalence of communicative disorders among prison inmates is considerably higher than that found in the general population, but research regarding inmate speech and hearing disorders is limited. This study investigated the nature and extent of communicative disorders in an inmate population of a medium-security penal institution in southeastern Virginia. The results of the screening indicated a slightly lower prevalence of stuttering, higher prevalences of articulation, voice, and hearing disorders, and more deficient receptive vocabulary skills than found in the general population. Some dialectal variations among black inmates are noted and the possible influence of linguistic-cultural interference on the results is discussed."}
{"id": "PMID:480936", "title": "A study of some factors affecting generalization of language training.", "content": "Two related experiments examined generalization across contexts involving different sentence forms when selected grammatical features were trained within a single syntactic context. Results obtained within a single-subject multiple baseline design showed that training the verbal/auxiliary (contracted with pronoun he) was sufficient to induce its generalization across a variety of verbs, object noun phrases, and a different subjective case pronoun, but not across an objective case pronoun. Similar results were obtained for the uncontractible auxiliary in the past tense. Generalization of the uncontractible auxiliary in the present tense was noted across different verbs; so also was the generalization of contractible copula to different adjectives qualifying the subjective case pronouns he and she (but not in qualifying an objective case pronoun it). Finally, generalization of possessive s inflection across male, female, and animal categories was evident. Trained behavior generalized to home situation in one of the experiments. Some unexpected results also suggest that training on certain forms of either verbal auxiliary or copula may be sufficient to generate correct production of both of them.", "contents": "A study of some factors affecting generalization of language training. Two related experiments examined generalization across contexts involving different sentence forms when selected grammatical features were trained within a single syntactic context. Results obtained within a single-subject multiple baseline design showed that training the verbal/auxiliary (contracted with pronoun he) was sufficient to induce its generalization across a variety of verbs, object noun phrases, and a different subjective case pronoun, but not across an objective case pronoun. Similar results were obtained for the uncontractible auxiliary in the past tense. Generalization of the uncontractible auxiliary in the present tense was noted across different verbs; so also was the generalization of contractible copula to different adjectives qualifying the subjective case pronouns he and she (but not in qualifying an objective case pronoun it). Finally, generalization of possessive s inflection across male, female, and animal categories was evident. Trained behavior generalized to home situation in one of the experiments. Some unexpected results also suggest that training on certain forms of either verbal auxiliary or copula may be sufficient to generate correct production of both of them."}
{"id": "PMID:480937", "title": "A comparison of language samples elicited at home and in the clinic.", "content": "One of the most common methods of obtaining diagnostic information about a child's language production is to collect and analyze a spontaneous language sample. Usually, this sample is collected in a clinical setting by a speech-language pathologist. Because children's language production seems very sensitive to situational variables, there is some question about the representativeness of language samples obtained in clinical settings. This study compared language samples obtained in the home by the mothers with those obtained in the clinic by speech-language pathologists. Subjects were three- to five-year-old children who had been referred for speech and language diagnostics. Results were that the children's mean-length-of-utterance (MLU) scores were better for the home sample, although there was no difference between their Developmental Sentence Scores (DSS) for the home sample and the clinic sample. The home samples yielded higher MLU stage placements (Brown, 1973) and higher estimated language ages on the DSS (Lee, 1974) for most of the children. Clinical implications of the results are discussed.", "contents": "A comparison of language samples elicited at home and in the clinic. One of the most common methods of obtaining diagnostic information about a child's language production is to collect and analyze a spontaneous language sample. Usually, this sample is collected in a clinical setting by a speech-language pathologist. Because children's language production seems very sensitive to situational variables, there is some question about the representativeness of language samples obtained in clinical settings. This study compared language samples obtained in the home by the mothers with those obtained in the clinic by speech-language pathologists. Subjects were three- to five-year-old children who had been referred for speech and language diagnostics. Results were that the children's mean-length-of-utterance (MLU) scores were better for the home sample, although there was no difference between their Developmental Sentence Scores (DSS) for the home sample and the clinic sample. The home samples yielded higher MLU stage placements (Brown, 1973) and higher estimated language ages on the DSS (Lee, 1974) for most of the children. Clinical implications of the results are discussed."}
{"id": "PMID:480938", "title": "Talker effects on word-discrimination scores of adults with sensorineural hearing impairment.", "content": "Speech discrimination testing was completed on 30 adults with varying degrees of sensorineural hearing impairment. Tape recordings of four talkers' utterances of CID W-22 word lists served as the stimulus materials. Listeners' responses were scored independently by three experienced judges. For 26 of the 30 subjects, the difference between the lowest and highest word-discrimination score was 8% or greater. Variations in scores could not be attributed to a single talker but were spread across all talkers. A moderate negative correlation was found between each listener's average word-discrimination score and amount of variability among his or her individual scores. The statistical analysis indicated that talker differences were responsible for only a small portion of the variability in scores and suggested that a factor of greater importance is the talker-listener interaction.", "contents": "Talker effects on word-discrimination scores of adults with sensorineural hearing impairment. Speech discrimination testing was completed on 30 adults with varying degrees of sensorineural hearing impairment. Tape recordings of four talkers' utterances of CID W-22 word lists served as the stimulus materials. Listeners' responses were scored independently by three experienced judges. For 26 of the 30 subjects, the difference between the lowest and highest word-discrimination score was 8% or greater. Variations in scores could not be attributed to a single talker but were spread across all talkers. A moderate negative correlation was found between each listener's average word-discrimination score and amount of variability among his or her individual scores. The statistical analysis indicated that talker differences were responsible for only a small portion of the variability in scores and suggested that a factor of greater importance is the talker-listener interaction."}
{"id": "PMID:480939", "title": "Management of palilalia with a pacing board.", "content": "Palilalia is a speaking disorder that has been likened to the festinating gait of Parkinsonian patients. This report describes a pacing device developed as a means of controlling the severely palilalic output of one patient. The device is a modeled after Luria's suggestion that a treatment program for such patients can be developed successfully by transferring automatic motor acts to a conscious, reactive level.", "contents": "Management of palilalia with a pacing board. Palilalia is a speaking disorder that has been likened to the festinating gait of Parkinsonian patients. This report describes a pacing device developed as a means of controlling the severely palilalic output of one patient. The device is a modeled after Luria's suggestion that a treatment program for such patients can be developed successfully by transferring automatic motor acts to a conscious, reactive level."}
{"id": "PMID:480940", "title": "Effects of four noise competitors on the California Consonant Test.", "content": "Thirty-five normal-hearing listeners' speech discrimination scores were obtained for the California Consonant Test (CCT) in four noise competitors: (1) a four talker complex (FT), (2) a nine-talker complex developed at Bowling Green State University (BGMTN), (3) cocktail party noise (CPN), and (4) white noise (WN). Five listeners received the CCT stimuli mixed ipsilaterally with each of the competing noises at one of seven different signal-to-noise ratios (S/Ns). Articulation functions were plotted for each noise competitor. Statistical analysis revealed that the noise types produced few differences on the CCT scores over most of the S/Ns tested, but that noise competitors similar to peripheral maskers (CPN and WN) had less effect on the scores at more severe levels than competitors more similar to perceptual maskers (FT and BGMTN). Results suggest that the CCT should be sufficiently difficult even without the present of a noise competitor for normal-hearing listeners in many audiologic testing situations. Levels that should approximate CCT maximum discrimination (D-Max) scores for normal listeners are suggested for use when clinic time does not permit the establishment of articulation functions. The clinician should determine the S/N of the CCT tape itself before establishing listening levels.", "contents": "Effects of four noise competitors on the California Consonant Test. Thirty-five normal-hearing listeners' speech discrimination scores were obtained for the California Consonant Test (CCT) in four noise competitors: (1) a four talker complex (FT), (2) a nine-talker complex developed at Bowling Green State University (BGMTN), (3) cocktail party noise (CPN), and (4) white noise (WN). Five listeners received the CCT stimuli mixed ipsilaterally with each of the competing noises at one of seven different signal-to-noise ratios (S/Ns). Articulation functions were plotted for each noise competitor. Statistical analysis revealed that the noise types produced few differences on the CCT scores over most of the S/Ns tested, but that noise competitors similar to peripheral maskers (CPN and WN) had less effect on the scores at more severe levels than competitors more similar to perceptual maskers (FT and BGMTN). Results suggest that the CCT should be sufficiently difficult even without the present of a noise competitor for normal-hearing listeners in many audiologic testing situations. Levels that should approximate CCT maximum discrimination (D-Max) scores for normal listeners are suggested for use when clinic time does not permit the establishment of articulation functions. The clinician should determine the S/N of the CCT tape itself before establishing listening levels."}
{"id": "PMID:480941", "title": "A comparison of children's performance on two tests of articulation.", "content": "This study compared children's performance on two tests of articulation. Children ranging in age from five years, four months to eleven years, six months were administered the Deep Test of Articulation and the Arizona Articulation Proficiency Scale: Revised. Comparisons between the tests were made of performance on individual sounds, total test performance, and differences in children identified as potentially in need of remediation. Results revealed differences in performance across tests on 15 of 21 sounds tested. Performance of almost 83% of the subjects varied across tests on at least one sound. Although some individual sounds displayed considerable variation between tests, total test performance and conclusions were similar. The data indicated, however, that the AAPS may fail to identify some children with consistent errors on relatively few sounds. The results are related to previous research, and clinical implications are discussed.", "contents": "A comparison of children's performance on two tests of articulation. This study compared children's performance on two tests of articulation. Children ranging in age from five years, four months to eleven years, six months were administered the Deep Test of Articulation and the Arizona Articulation Proficiency Scale: Revised. Comparisons between the tests were made of performance on individual sounds, total test performance, and differences in children identified as potentially in need of remediation. Results revealed differences in performance across tests on 15 of 21 sounds tested. Performance of almost 83% of the subjects varied across tests on at least one sound. Although some individual sounds displayed considerable variation between tests, total test performance and conclusions were similar. The data indicated, however, that the AAPS may fail to identify some children with consistent errors on relatively few sounds. The results are related to previous research, and clinical implications are discussed."}
{"id": "PMID:480942", "title": "Bilateral cord dysfunctions: abductor type spastic dysphonia.", "content": "Unlike cord approximation disorders such as adductor type spastic dysphonia and vocal cord paralysis, which have been well defined, abductor type spastic dysphonia has received limited attention. This report presents voice symptoms, spectrographic data, indirect laryngoscopic observations, and case histories of two cases. Questions are raised as to whether there is a spastic component to this disorder, and if there is a specific age group affected.", "contents": "Bilateral cord dysfunctions: abductor type spastic dysphonia. Unlike cord approximation disorders such as adductor type spastic dysphonia and vocal cord paralysis, which have been well defined, abductor type spastic dysphonia has received limited attention. This report presents voice symptoms, spectrographic data, indirect laryngoscopic observations, and case histories of two cases. Questions are raised as to whether there is a spastic component to this disorder, and if there is a specific age group affected."}
{"id": "PMID:480943", "title": "Mirror, mirror on the wall...?", "content": "This study was designed to determine the value of using a mirror for speech teaching. Data derived from manner, place, voicing, and blend errors produced by 27 hearing-impaired children while imitating consonant-vowel syllables under three conditions (audition alone, audition plus direct vision, audition plus vision using a mirror) were analyzed to determine whether the use of a mirror enhances or detracted from the accuracy of their responses relative to the other two conditions; whether different types of errors were made under any of the conditions; and whether error types under each condition were related to age or hearing level. Although more place errors occurred under the auditory condition, the difference between the numbers of errors made under the two auditory-visual conditions was not significant. The use of a mirror neither enhanced nor detracted from the accuracy of responses of subjects in this study. This finding cast doubt upon the value of using a mirror as a general aid in speech teaching.", "contents": "Mirror, mirror on the wall...? This study was designed to determine the value of using a mirror for speech teaching. Data derived from manner, place, voicing, and blend errors produced by 27 hearing-impaired children while imitating consonant-vowel syllables under three conditions (audition alone, audition plus direct vision, audition plus vision using a mirror) were analyzed to determine whether the use of a mirror enhances or detracted from the accuracy of their responses relative to the other two conditions; whether different types of errors were made under any of the conditions; and whether error types under each condition were related to age or hearing level. Although more place errors occurred under the auditory condition, the difference between the numbers of errors made under the two auditory-visual conditions was not significant. The use of a mirror neither enhanced nor detracted from the accuracy of responses of subjects in this study. This finding cast doubt upon the value of using a mirror as a general aid in speech teaching."}
{"id": "PMID:480944", "title": "An examination of variables involved in tympanometric assessment of eustachian tube function in adults.", "content": "Eustachian tube function was assessed tympanometrically in a group of normal adults. A pressure-swallow technique of assessing Eustachian tube ventilatory function was administered with positive and negative induced pressures in the range of +/- 200 mm to +/- 400 mm H2O. This study indicated the relative efficiency of measurement of Eustachian tube function under each of the experimental conditions. Recommendations of procedures for further clinical data collection are presented.", "contents": "An examination of variables involved in tympanometric assessment of eustachian tube function in adults. Eustachian tube function was assessed tympanometrically in a group of normal adults. A pressure-swallow technique of assessing Eustachian tube ventilatory function was administered with positive and negative induced pressures in the range of +/- 200 mm to +/- 400 mm H2O. This study indicated the relative efficiency of measurement of Eustachian tube function under each of the experimental conditions. Recommendations of procedures for further clinical data collection are presented."}
{"id": "PMID:480949", "title": "Comparison of DES vs DES + chlorambucil in women with first recurrence of breast cancer.", "content": "Thirty-four women with first recurrence of breast cancer were randomized into two groups, and received either Diethylstilbestrol (DES) 5 mg orally (PO) t.i.d. alone, or in combination with Chlorambucil (CB) 0.1--0.2 mg/kg/day PO. All patients randomized were greater than 5 years postmenopausal at the time of the study and had no prior chemical or hormonal therapy. Estrogen receptors were not available. There was no significant difference between Groups A and B with respect to frequency of objective response or mean duration of that response, with the values for Group A being 46.2% and 4.8 months, respectively, and for Group B, 46.7% and 4.8 months (P greater than 0.05). The most common toxicities noted for both groups were nausea and vomiting, edema, weakness, and thrombophlebitis. The risk of major toxicity necessitating withdrawal from the study was greater in Group B due to the added danger of thrombocytopenia/pancytopenia. The addition of CB to DES does not appear to offer any significant advantage over DES alone in women with first recurrence of breast cancer.", "contents": "Comparison of DES vs DES + chlorambucil in women with first recurrence of breast cancer. Thirty-four women with first recurrence of breast cancer were randomized into two groups, and received either Diethylstilbestrol (DES) 5 mg orally (PO) t.i.d. alone, or in combination with Chlorambucil (CB) 0.1--0.2 mg/kg/day PO. All patients randomized were greater than 5 years postmenopausal at the time of the study and had no prior chemical or hormonal therapy. Estrogen receptors were not available. There was no significant difference between Groups A and B with respect to frequency of objective response or mean duration of that response, with the values for Group A being 46.2% and 4.8 months, respectively, and for Group B, 46.7% and 4.8 months (P greater than 0.05). The most common toxicities noted for both groups were nausea and vomiting, edema, weakness, and thrombophlebitis. The risk of major toxicity necessitating withdrawal from the study was greater in Group B due to the added danger of thrombocytopenia/pancytopenia. The addition of CB to DES does not appear to offer any significant advantage over DES alone in women with first recurrence of breast cancer."}
{"id": "PMID:480950", "title": "Fibrosarcoma of the mediastinum.", "content": "Fibrosarcoma is a rare primary malignant tumor of the mediastinum. Three cases are presented with different presenting symptoms and clinical manifestations. Thoracotomy with biopsy of the mass is the only method for arriving at a definitive histologic diagnosis.", "contents": "Fibrosarcoma of the mediastinum. Fibrosarcoma is a rare primary malignant tumor of the mediastinum. Three cases are presented with different presenting symptoms and clinical manifestations. Thoracotomy with biopsy of the mass is the only method for arriving at a definitive histologic diagnosis."}
{"id": "PMID:480951", "title": "Adjuvant chemoimmunotherapy following regional therapy for isolated recurrences of breast cancer (stage IV NED).", "content": "A combination of 5-fluorouracil, Adriamycin, cyclophosphamide, and BCG (FAC-BCG) was evaluated as adjuvant treatment in breast cancer patients following surgical excision and/or radiation of first site of recurrent disease. In a group of 68 patients treated with FAC-BCG, the estimated proportion remaining free of disease at 2 years from first recurrence was 69%, compared to 24% in 60 historical control patients (P less than 0.01). Estimated 2-year survival rate from first recurrence was 85% for the FAC-BCG patients and 78% for the controls (P = 0.07). This regimen has significantly prolonged the disease-free interval from the first recurrence, but additional follow-up is needed to determine its effect on long-term survival.", "contents": "Adjuvant chemoimmunotherapy following regional therapy for isolated recurrences of breast cancer (stage IV NED). A combination of 5-fluorouracil, Adriamycin, cyclophosphamide, and BCG (FAC-BCG) was evaluated as adjuvant treatment in breast cancer patients following surgical excision and/or radiation of first site of recurrent disease. In a group of 68 patients treated with FAC-BCG, the estimated proportion remaining free of disease at 2 years from first recurrence was 69%, compared to 24% in 60 historical control patients (P less than 0.01). Estimated 2-year survival rate from first recurrence was 85% for the FAC-BCG patients and 78% for the controls (P = 0.07). This regimen has significantly prolonged the disease-free interval from the first recurrence, but additional follow-up is needed to determine its effect on long-term survival."}
{"id": "PMID:480952", "title": "Tumorlytic activity of autochthonous blood fractions upon spontaneous murine mammary carcinomas.", "content": "Autologous blood fractions of mice bearing spontaneous mammary carcinoma were assessed for their autotumorlytic capability. In the 180 animals evaluated, it appears that in the blood of these tumor animals there coexists at least two types of biological antagonists ie, one which is capable of inducing tumor breakdown and necrosis and the other which apparently nullifies this action. These results seem additive to the growing body of evidence that the tumorous host has the capability of destroying its own neoplasm but some negative homeostatic mechanism seems to favor the tumor rather than the host.", "contents": "Tumorlytic activity of autochthonous blood fractions upon spontaneous murine mammary carcinomas. Autologous blood fractions of mice bearing spontaneous mammary carcinoma were assessed for their autotumorlytic capability. In the 180 animals evaluated, it appears that in the blood of these tumor animals there coexists at least two types of biological antagonists ie, one which is capable of inducing tumor breakdown and necrosis and the other which apparently nullifies this action. These results seem additive to the growing body of evidence that the tumorous host has the capability of destroying its own neoplasm but some negative homeostatic mechanism seems to favor the tumor rather than the host."}
{"id": "PMID:480953", "title": "Non-specific and specific active immunotherapy in a B16 murine melanoma system.", "content": "Conflicts amongst reports concerning the efficacy of both nonspecific and specific attempts at immunotherapy may be ascribed to different animal models utilizing tumors of different immunogenicity. We have selected the B16 mouse melanoma model as the example of a spontaneously occurring neoplasm that is histocompatible with the host and does have tumor-associated antigens. Attempts to alter tumor growth or survival with nonspecific active immunotherapy as well as with specific active immunotherapy were not successful. Nonspecific active pre-immunization failed to alter tumor take or growth. Specific active immunotherapy both with and without adjuvant did decrease tumor take and prolong host survival. The effects were increasingly documented at lower tumor cell inoculums and became less apparent with increase in the tumor cell challenge.", "contents": "Non-specific and specific active immunotherapy in a B16 murine melanoma system. Conflicts amongst reports concerning the efficacy of both nonspecific and specific attempts at immunotherapy may be ascribed to different animal models utilizing tumors of different immunogenicity. We have selected the B16 mouse melanoma model as the example of a spontaneously occurring neoplasm that is histocompatible with the host and does have tumor-associated antigens. Attempts to alter tumor growth or survival with nonspecific active immunotherapy as well as with specific active immunotherapy were not successful. Nonspecific active pre-immunization failed to alter tumor take or growth. Specific active immunotherapy both with and without adjuvant did decrease tumor take and prolong host survival. The effects were increasingly documented at lower tumor cell inoculums and became less apparent with increase in the tumor cell challenge."}
{"id": "PMID:480960", "title": "Repair of double-outlet right ventricle. An analysis of 62 cases.", "content": "Sixty-two patients with double-outlet right ventricle (DORV) underwent complete intracardiac repair between 1967 and July, 1978. Five patients (three deaths) with DORV and complete atrioventricular (AV) canal are the subject of a separate report and are not discussed further here. Twenty-eight patients had relatively uncomplicated DORV (subaortic ventricular septal defect [VSD], doubly committed VSD, or 1-malposition of the aorta with subaortic VSD) with or without pulmonary stenosis. Two (14%) of 14 died after a completely intraventricular repair, and one (12%) of eight after repair including use of a valved external conduit. Use of a transannular patch in this group was an incremental risk factor, five (83%) dying among six treated in this way. Anterior enlargement of a restrictive or unfavorably located VSD did not increase risk. A noncommitted VSD was an incremental risk factor, two (50%) of four dying after repair. No instances of complete heart block occurred in these groups. Two late deaths occurred in the uncomplicated group, from persisting servere pulmonary hypertension. The late results were good in the remainder and in the two surviving patients with noncommitted VSD. After repair of the Taussig-Bing type of DORV, eight deaths (32%) occurred among 25 patients. Complete heart block developed in two patients. In the Taussig-Bing type of DORV, the 6 year actuarial survival rate was only 38%. Most late deaths were related to improtant pulmonary vascular disease. The surgical technique that has evolved for the basic tunnel repair in the various types of DORV is described.", "contents": "Repair of double-outlet right ventricle. An analysis of 62 cases. Sixty-two patients with double-outlet right ventricle (DORV) underwent complete intracardiac repair between 1967 and July, 1978. Five patients (three deaths) with DORV and complete atrioventricular (AV) canal are the subject of a separate report and are not discussed further here. Twenty-eight patients had relatively uncomplicated DORV (subaortic ventricular septal defect [VSD], doubly committed VSD, or 1-malposition of the aorta with subaortic VSD) with or without pulmonary stenosis. Two (14%) of 14 died after a completely intraventricular repair, and one (12%) of eight after repair including use of a valved external conduit. Use of a transannular patch in this group was an incremental risk factor, five (83%) dying among six treated in this way. Anterior enlargement of a restrictive or unfavorably located VSD did not increase risk. A noncommitted VSD was an incremental risk factor, two (50%) of four dying after repair. No instances of complete heart block occurred in these groups. Two late deaths occurred in the uncomplicated group, from persisting servere pulmonary hypertension. The late results were good in the remainder and in the two surviving patients with noncommitted VSD. After repair of the Taussig-Bing type of DORV, eight deaths (32%) occurred among 25 patients. Complete heart block developed in two patients. In the Taussig-Bing type of DORV, the 6 year actuarial survival rate was only 38%. Most late deaths were related to improtant pulmonary vascular disease. The surgical technique that has evolved for the basic tunnel repair in the various types of DORV is described."}
{"id": "PMID:480961", "title": "Preoperative prediction from cineangiograms of postrepair right ventricular pressure in tetralogy of Fallot.", "content": "To aid preoperative decision-making, we have related the ratio of postrepair peak pressure in the right and left ventricles (PRV/LV) to preoperative cineangiographic measurements in a retrospective study of 135 patients undergoing complete repair of tetralogy of Fallot or tetralogy of Fallot with pulmonary atresia. Postrepair PRV/LV was related to the preoperative diameter of right (DRPA) and left (DLPA) pulmonary arteries normalized to the descending thoracic aorta (DescThAo) in patients undergoing repair with transannular patching or a valved external conduit by the dquation: PRV/LV = 0.4840/(DRPA/DescThAO + DLPA/DesThAo) + 0.2007. Stenosis of the right pulmonary artery orifice and pulmonary artery arborization abnormalities incrementally increased postrepair PRV/LV. When a transannular patch was not used in classical tetralogy of Fallot, an increment of postrepair PRV/LV usually resulted, depending upon the size of the \"anulus\" measured intraoperatively: Incremental PRV/LV = 0.09437 . exp(-0.6344 . Z) where Z is a normalized expression in circumference terms of the diameter of the pulmonary arterial outflow tract (DPAOT) measured intraoperatively after infundibular dissection and valvotomy. DPAOT is itself related to the cineangiographically measured pulmonary valve anulus diameter (DPVA): DPAOT = 3.357 . DPVA0.5789 . BSA0.1551. In toto, these relations allow postrepair PRV/LV without transannular patching to be estimated from preoperative cineangiographic measurements. This allows preoperative predictiom in classical tetralogy of Fallot of the need for transannular patching, and in infants this can determine the choice between primary one-stage repair and two-stage repair. Prediction of postrepair PRV/LV when transannular patching or an external conduit is planned allows identification of patients in whom right and left pulmonary arteries are too small for safe complete repair, and in them an initial palliative operation should be done to enlarge the arteries.", "contents": "Preoperative prediction from cineangiograms of postrepair right ventricular pressure in tetralogy of Fallot. To aid preoperative decision-making, we have related the ratio of postrepair peak pressure in the right and left ventricles (PRV/LV) to preoperative cineangiographic measurements in a retrospective study of 135 patients undergoing complete repair of tetralogy of Fallot or tetralogy of Fallot with pulmonary atresia. Postrepair PRV/LV was related to the preoperative diameter of right (DRPA) and left (DLPA) pulmonary arteries normalized to the descending thoracic aorta (DescThAo) in patients undergoing repair with transannular patching or a valved external conduit by the dquation: PRV/LV = 0.4840/(DRPA/DescThAO + DLPA/DesThAo) + 0.2007. Stenosis of the right pulmonary artery orifice and pulmonary artery arborization abnormalities incrementally increased postrepair PRV/LV. When a transannular patch was not used in classical tetralogy of Fallot, an increment of postrepair PRV/LV usually resulted, depending upon the size of the \"anulus\" measured intraoperatively: Incremental PRV/LV = 0.09437 . exp(-0.6344 . Z) where Z is a normalized expression in circumference terms of the diameter of the pulmonary arterial outflow tract (DPAOT) measured intraoperatively after infundibular dissection and valvotomy. DPAOT is itself related to the cineangiographically measured pulmonary valve anulus diameter (DPVA): DPAOT = 3.357 . DPVA0.5789 . BSA0.1551. In toto, these relations allow postrepair PRV/LV without transannular patching to be estimated from preoperative cineangiographic measurements. This allows preoperative predictiom in classical tetralogy of Fallot of the need for transannular patching, and in infants this can determine the choice between primary one-stage repair and two-stage repair. Prediction of postrepair PRV/LV when transannular patching or an external conduit is planned allows identification of patients in whom right and left pulmonary arteries are too small for safe complete repair, and in them an initial palliative operation should be done to enlarge the arteries."}
{"id": "PMID:480963", "title": "Sinus venosus type of atrial septal defect with partial anomalous pulmonary venous return.", "content": "This report describes our method of repairing the sinus venosus type of atrial septal defects. The superior vena cava is compartmentatlized by means of a single double-armed suture. Caval catheters are used as temporary internal shunts, and a vertical U-shaped patch is incorporated into the atriotomy suture line. The experiences with 27 cases are presented.", "contents": "Sinus venosus type of atrial septal defect with partial anomalous pulmonary venous return. This report describes our method of repairing the sinus venosus type of atrial septal defects. The superior vena cava is compartmentatlized by means of a single double-armed suture. Caval catheters are used as temporary internal shunts, and a vertical U-shaped patch is incorporated into the atriotomy suture line. The experiences with 27 cases are presented."}
{"id": "PMID:480964", "title": "Coronary sinus septal defect. Surgical considerations.", "content": "The coronary sinus septal defect is an uncommon anomaly. If it is not suspected, found, and repaired at operation, a residual intracardiac shunt will result. The defect usually is associated with a left superior vena cava (LSVC), which necessitates special consideration in the conduct of cardiopulmonary bypass. Our recent treatment of three cases of coronary sinus septal defect stimulated us to review the embryology, anatomy, diagnosis, and surgical treatment of this malformation.", "contents": "Coronary sinus septal defect. Surgical considerations. The coronary sinus septal defect is an uncommon anomaly. If it is not suspected, found, and repaired at operation, a residual intracardiac shunt will result. The defect usually is associated with a left superior vena cava (LSVC), which necessitates special consideration in the conduct of cardiopulmonary bypass. Our recent treatment of three cases of coronary sinus septal defect stimulated us to review the embryology, anatomy, diagnosis, and surgical treatment of this malformation."}
{"id": "PMID:480965", "title": "Surgical repair of inferior sinus venosus atrial septal defect.", "content": "A 9-year-old girl presented for cardiac evaluation with symptoms of dyspena, fatigue, and cyanosis with exercise. Cardiac catheterization demonstrated an atrial septal defect; an anomalous right superior pulmonary vein was suspected but not confirmed. Operation disclosed anomalous drainage of the right superior and inferior pulmonary veins into the right atrium, an intact fossa ovalis, and an inferior sinus venosus defect. Repair was accomplished by detaching the posterior edge of the atrial septum and suturing it to the right of the pulmonary veins, so that the defect was closed and all the pulmonary venous blood was directed to the left atrium.", "contents": "Surgical repair of inferior sinus venosus atrial septal defect. A 9-year-old girl presented for cardiac evaluation with symptoms of dyspena, fatigue, and cyanosis with exercise. Cardiac catheterization demonstrated an atrial septal defect; an anomalous right superior pulmonary vein was suspected but not confirmed. Operation disclosed anomalous drainage of the right superior and inferior pulmonary veins into the right atrium, an intact fossa ovalis, and an inferior sinus venosus defect. Repair was accomplished by detaching the posterior edge of the atrial septum and suturing it to the right of the pulmonary veins, so that the defect was closed and all the pulmonary venous blood was directed to the left atrium."}
{"id": "PMID:480966", "title": "Successful surgical correction of symptomatic cor triatriatum dexter.", "content": "An unusual case of cor triatriatum dexter is presented. Abnormal embryologic development of the eustachian valve caused nearly complete septation of the right atrium in a 67-year-old woman. Multiple supraventricular arrhythmias prompted clinical evaluation and diagnosis. Surgical excision of the anomalous membrane resulted in complete relief from arrhythmias and a clinical cure. Cor triatriatum dexter is rare and is infrequently diagnosed before postmortem study; however, once the diagnosis is extablished, the condition is amenable to a relatively simple surgical correction. Supraventricular tachycardia has not previously been reported to be associated with cor triatriatum dexter. We believe this is the first case in which such arrhythmias led to early diagnosis and successful surgical correction.", "contents": "Successful surgical correction of symptomatic cor triatriatum dexter. An unusual case of cor triatriatum dexter is presented. Abnormal embryologic development of the eustachian valve caused nearly complete septation of the right atrium in a 67-year-old woman. Multiple supraventricular arrhythmias prompted clinical evaluation and diagnosis. Surgical excision of the anomalous membrane resulted in complete relief from arrhythmias and a clinical cure. Cor triatriatum dexter is rare and is infrequently diagnosed before postmortem study; however, once the diagnosis is extablished, the condition is amenable to a relatively simple surgical correction. Supraventricular tachycardia has not previously been reported to be associated with cor triatriatum dexter. We believe this is the first case in which such arrhythmias led to early diagnosis and successful surgical correction."}
{"id": "PMID:480967", "title": "Calculation of systemic blood flow with pulmonary artery thermistor probe.", "content": "A simple thermodilution technique is described for determining systemic blood flow with right atrial and left atrial catheters and a pulmonary arterial thermistor probe. Injections of cold water into the atrial catheters provide computer display readings that permit direct calculation of systemic blood flow. The method is convenient for determining systemic blood flow in postoperative patients with a residual left-to-right shunt.", "contents": "Calculation of systemic blood flow with pulmonary artery thermistor probe. A simple thermodilution technique is described for determining systemic blood flow with right atrial and left atrial catheters and a pulmonary arterial thermistor probe. Injections of cold water into the atrial catheters provide computer display readings that permit direct calculation of systemic blood flow. The method is convenient for determining systemic blood flow in postoperative patients with a residual left-to-right shunt."}
{"id": "PMID:480968", "title": "Congenital tracheoesophageal fistula without esophageal atresia.", "content": "Congenital tracheoesophageal fistula (TEF) without esophageal atresia, commonly referred to as H-type fistula, comprises only 4.2% of all TEF's. Four infants with this entity were surgically treated at Santa Rosa Children's Hospital during the period of 1974 to 1977, and their diagnosis, surgical management, and hospital course are described in detail. H-type TEF is characterized by a clinical triad consisting of paroxysms of coughing precipitated by feeding, gaseous distention of the gastrointestinal tract, and pneumonitis. Demonstration of H-type TEF can be difficult and may be accomplished only by repeated examinations. The esophagogram using cinefluoroscopy and image intensification is the primary diagnostic technique utilized. If this is nondiagnostic, then tracheobronchoscopy should be performed in conjunction with ancillary techniques which improve endoscopic yield. Surgical correction can be accomplished through a cervical approach in 80% of cases. Preoperatival treatment of H-type TEF is dependent upon a high index of suspicion, an aggressive diagnostic approach, and prompt surgical correction. Our series of patients demonstrates that early diagnosis is feasible.", "contents": "Congenital tracheoesophageal fistula without esophageal atresia. Congenital tracheoesophageal fistula (TEF) without esophageal atresia, commonly referred to as H-type fistula, comprises only 4.2% of all TEF's. Four infants with this entity were surgically treated at Santa Rosa Children's Hospital during the period of 1974 to 1977, and their diagnosis, surgical management, and hospital course are described in detail. H-type TEF is characterized by a clinical triad consisting of paroxysms of coughing precipitated by feeding, gaseous distention of the gastrointestinal tract, and pneumonitis. Demonstration of H-type TEF can be difficult and may be accomplished only by repeated examinations. The esophagogram using cinefluoroscopy and image intensification is the primary diagnostic technique utilized. If this is nondiagnostic, then tracheobronchoscopy should be performed in conjunction with ancillary techniques which improve endoscopic yield. Surgical correction can be accomplished through a cervical approach in 80% of cases. Preoperatival treatment of H-type TEF is dependent upon a high index of suspicion, an aggressive diagnostic approach, and prompt surgical correction. Our series of patients demonstrates that early diagnosis is feasible."}
{"id": "PMID:480969", "title": "Gas exchange after pulmonary fat embolism in dogs.", "content": "Gas exchange following moderately severe experimental pulmonary fat embolism was studied in nine dogs. A new method designed to describe the distribution of ventilation-perfusion ratios in the lung was applied before and after intravenous injection of homologous neutral fat. The dose of fat (0.75 ml/kg) was low enough to produce a small but significant decrease in arterial PO2 (mean change of 10 mm Hg) in the first 15 minutes after the embolism but high enough to result in the death of two of the four dogs that were allowed to survive the initial postembolism period. Pulmonary artery pressure and pulmonary vascular resistance both rose significantly within 5 minutes of the fat injection and remained elevated for the 2 hour experimental period. Immediately after the embolism there was an increase in the percentage of the total ventilation going to areas of the lung with ventilation-perfusion ratios between 10 and 100, which usually appeared as a discrete mode in the ventilation distribution. This mismatching of ventilation and perfusion partially resolved within 2 hours after the embolism, as indicated by the gradual disappearance of this population of gas exchanging units with relatively decreased blood flow. At no time within 2 hours after the embolism was there a significant increase in shunt or in ventilation to totally unperfused lung. The gas exchange pattern in the two dogs that subsequently died was indistinguishable from that of the other seven in the immediate postembolism period.", "contents": "Gas exchange after pulmonary fat embolism in dogs. Gas exchange following moderately severe experimental pulmonary fat embolism was studied in nine dogs. A new method designed to describe the distribution of ventilation-perfusion ratios in the lung was applied before and after intravenous injection of homologous neutral fat. The dose of fat (0.75 ml/kg) was low enough to produce a small but significant decrease in arterial PO2 (mean change of 10 mm Hg) in the first 15 minutes after the embolism but high enough to result in the death of two of the four dogs that were allowed to survive the initial postembolism period. Pulmonary artery pressure and pulmonary vascular resistance both rose significantly within 5 minutes of the fat injection and remained elevated for the 2 hour experimental period. Immediately after the embolism there was an increase in the percentage of the total ventilation going to areas of the lung with ventilation-perfusion ratios between 10 and 100, which usually appeared as a discrete mode in the ventilation distribution. This mismatching of ventilation and perfusion partially resolved within 2 hours after the embolism, as indicated by the gradual disappearance of this population of gas exchanging units with relatively decreased blood flow. At no time within 2 hours after the embolism was there a significant increase in shunt or in ventilation to totally unperfused lung. The gas exchange pattern in the two dogs that subsequently died was indistinguishable from that of the other seven in the immediate postembolism period."}
{"id": "PMID:480970", "title": "Surgical implications of pulmonary actinomycosis.", "content": "Four cases of pulmonary actinomycosis are presented. Resection was necessary in three cases before the diagnosis was established. A high index of suspicion for this disease is advised for the thoracic surgeon involved in the treatment of lung masses.", "contents": "Surgical implications of pulmonary actinomycosis. Four cases of pulmonary actinomycosis are presented. Resection was necessary in three cases before the diagnosis was established. A high index of suspicion for this disease is advised for the thoracic surgeon involved in the treatment of lung masses."}
{"id": "PMID:480972", "title": "Thoracoscopy: new method of early diagnosis of cardiac herniation.", "content": "Cardiac herniation is an uncommon but rapidly fatal complication of radical pneumonectomy. Although operative correction is simple, a successful clinical outcome requires early diagnosis and thoracotomy. A patient is described in whom a left-sided cardiac herniation through a pericardial defect occurred following radical pneumonectomy. The diagnosis was suspected by persistent hypotension and radiographic changes. Confirmation of the diagnosis was achieved by thoracoscopy, performed with local anesthesia in the operating room. Immediate thoracotomy resulted in a successful outcome.", "contents": "Thoracoscopy: new method of early diagnosis of cardiac herniation. Cardiac herniation is an uncommon but rapidly fatal complication of radical pneumonectomy. Although operative correction is simple, a successful clinical outcome requires early diagnosis and thoracotomy. A patient is described in whom a left-sided cardiac herniation through a pericardial defect occurred following radical pneumonectomy. The diagnosis was suspected by persistent hypotension and radiographic changes. Confirmation of the diagnosis was achieved by thoracoscopy, performed with local anesthesia in the operating room. Immediate thoracotomy resulted in a successful outcome."}
{"id": "PMID:480987", "title": "Clinical application of a new endoscopic technique for detection of in situ bronchial carcinoma.", "content": "Hematoporphyrin is preferentially concentrated by cancer tissue and therefore is a potentially useful chemical marker. This report demonstrates the effectiveness of a photoelectric hematoporphyrin fluorescence detector used simultaneously with flexible fiberoptic bronchoscopy as a means of detecting and localizing bronchogenic carcinoma. The detector generates an audio signal when fluorescence is within the visual field of the bronchoscope. Of interest is that an audio signal was generated which indicated hematoporphyrin fluorescence in areas where no mucosal abnormality had been seen. These areas proved to be carcinoma in situ.", "contents": "Clinical application of a new endoscopic technique for detection of in situ bronchial carcinoma. Hematoporphyrin is preferentially concentrated by cancer tissue and therefore is a potentially useful chemical marker. This report demonstrates the effectiveness of a photoelectric hematoporphyrin fluorescence detector used simultaneously with flexible fiberoptic bronchoscopy as a means of detecting and localizing bronchogenic carcinoma. The detector generates an audio signal when fluorescence is within the visual field of the bronchoscope. Of interest is that an audio signal was generated which indicated hematoporphyrin fluorescence in areas where no mucosal abnormality had been seen. These areas proved to be carcinoma in situ."}
{"id": "PMID:480988", "title": "The continent ileostomy: a viable alternative.", "content": "The continent ileostomy is one of the alternative techniques in managing patients who require a proctocolectomy. Although previously plagued with up to 30% revisions, the continent ileostomy appears to contribute significantly to the social and psychologic well-being of these patients. Our experience suggests that improved surgical techniques make this procedure a safe and favorable alternative to the traditional Brooke ileostomy.", "contents": "The continent ileostomy: a viable alternative. The continent ileostomy is one of the alternative techniques in managing patients who require a proctocolectomy. Although previously plagued with up to 30% revisions, the continent ileostomy appears to contribute significantly to the social and psychologic well-being of these patients. Our experience suggests that improved surgical techniques make this procedure a safe and favorable alternative to the traditional Brooke ileostomy."}
{"id": "PMID:480989", "title": "Effect of elective induction on birth weight.", "content": "The 1,250 Mayo Clinic obstetric deliveries for 1976 were reviewed and 73 elective inductions were identified. Sixty-three of these mothers were successfully matched by gravidity, parity, and age within 5 years with healthy women in whom delivery was spontaneous at term. The mean birth weight for the electively delivered infants was greater than that for the controls, but the difference was not statistically significant. Mean gestation and 1-minute Apgar scores were similar for both groups. The neonatal problems observed, with the exception of elevated bilirubin levels, did not seem to point to any special hazard of induction. Even though pregnancy is interrupted, elective induction, as practiced at the Mayo Clinic, was not shown to produce a systematic reduction in birth weight.", "contents": "Effect of elective induction on birth weight. The 1,250 Mayo Clinic obstetric deliveries for 1976 were reviewed and 73 elective inductions were identified. Sixty-three of these mothers were successfully matched by gravidity, parity, and age within 5 years with healthy women in whom delivery was spontaneous at term. The mean birth weight for the electively delivered infants was greater than that for the controls, but the difference was not statistically significant. Mean gestation and 1-minute Apgar scores were similar for both groups. The neonatal problems observed, with the exception of elevated bilirubin levels, did not seem to point to any special hazard of induction. Even though pregnancy is interrupted, elective induction, as practiced at the Mayo Clinic, was not shown to produce a systematic reduction in birth weight."}
{"id": "PMID:480990", "title": "Arteriovenous malformation affecting the transverse dural venous sinus--an acquired lesion.", "content": "Dural arteriovenous malformations of the transverse sinus are relatively well localized to the occipitomastoid regions and occur predominantly in women who are more than 40 years of age. The signs and symptoms, except for pulsatile tinnitus, vary and apparently depend on the presence of impaired venous drainage, which may produce increased intracranial pressure, papilledema, and transient or persistent neurologic deficits. Eight of the 14 patients described had occlusion of one or both transverse or sigmoid sinuses. Two patients had angiographic demonstration of sigmoid or transverse sinus occlusion (one 1 year and the other 4 years) before the development of an arteriovenous malformation in the appropriate sinus. Dural sinus occlusion may precede the development of a dural arteriovenous malformation, and the pathogenesis may be partial recanalization of a thrombus.", "contents": "Arteriovenous malformation affecting the transverse dural venous sinus--an acquired lesion. Dural arteriovenous malformations of the transverse sinus are relatively well localized to the occipitomastoid regions and occur predominantly in women who are more than 40 years of age. The signs and symptoms, except for pulsatile tinnitus, vary and apparently depend on the presence of impaired venous drainage, which may produce increased intracranial pressure, papilledema, and transient or persistent neurologic deficits. Eight of the 14 patients described had occlusion of one or both transverse or sigmoid sinuses. Two patients had angiographic demonstration of sigmoid or transverse sinus occlusion (one 1 year and the other 4 years) before the development of an arteriovenous malformation in the appropriate sinus. Dural sinus occlusion may precede the development of a dural arteriovenous malformation, and the pathogenesis may be partial recanalization of a thrombus."}
{"id": "PMID:480991", "title": "Digitalis glycosides.", "content": "The digitalis glycosides number among the most commonly used cardiovascular drugs. Despite 200 years of investigation, much new information regarding their mechanisms of action and clinical use continues to be developed. Clinicians dispensing these potent drugs need to be aware of thes advances if they are to make the best possible therapeutic decisions.", "contents": "Digitalis glycosides. The digitalis glycosides number among the most commonly used cardiovascular drugs. Despite 200 years of investigation, much new information regarding their mechanisms of action and clinical use continues to be developed. Clinicians dispensing these potent drugs need to be aware of thes advances if they are to make the best possible therapeutic decisions."}
{"id": "PMID:480992", "title": "Demyelination of the brain in tuberous sclerosis: computed tomography evidence.", "content": "Two patients with tuberous sclerosis are described in whom computed tomography of the head revealed areas of diffuse diminution of density suggestive of demyelination. Computed tomography is a sensitive test for the detection of intracerebral lesions in tuberous sclerosis, and the finding of areas of diminished density in the absence of evidence for tumor or ventricular obstruction correlates with the common histologic finding of diffuse demyelination. Serial studies with computed tomography are likely to determine whether such demyelination precedes the growth of hamartomas and calcification that characterize tuberous sclerosis.", "contents": "Demyelination of the brain in tuberous sclerosis: computed tomography evidence. Two patients with tuberous sclerosis are described in whom computed tomography of the head revealed areas of diffuse diminution of density suggestive of demyelination. Computed tomography is a sensitive test for the detection of intracerebral lesions in tuberous sclerosis, and the finding of areas of diminished density in the absence of evidence for tumor or ventricular obstruction correlates with the common histologic finding of diffuse demyelination. Serial studies with computed tomography are likely to determine whether such demyelination precedes the growth of hamartomas and calcification that characterize tuberous sclerosis."}
{"id": "PMID:480994", "title": "Neuronal involution during ageing. Ultrastructural study in the rat cerebellum.", "content": "Involutive phenomena have been investigated by electron microscopy in the Purkinje Pk neuron of the cerebellar cortex of the aging rat. The still limited number of specimens available to date, however, suggest an age-related progression of morphological and functional deteriorations involving particularly the intraneuronal \"nucleus-ribosome system\" (NRS). The impairments are characterized by changes in the nucleolar texture. These alterations are accompanied by modifications in the repartition and relative proportion of RNP components of the nucleolus. In addition, other nuclear elements such as interchromatin and perichromatin granules may vary in importance with age. Recognizable changes in the ribosomal constituents of the NRS are evidenced by modifications in the density and distribution of free ribosomes. An altered structure and organization of GER cisternae are also evident. Furthermore, \"light\" cytoplasmic areas, an increased evidence of neurotubules and the gradual congestion of the pericaryon by age pigments are other valuable ultrastructural features that may be regarded as part of the sequence of morphologic events occurring during neuonal ageing. The above ultrastructural data will subsequently form the basis of a model of ageing in the nerve cell, which will complete the previously proposed model of neuronal maturation. Therefore, this long-term study essentially purports the investigation of subcellular events taking place in the Pk neuron all along the normal life span in rats. This model will also be used to evaluate the changes in the sequence and the reinforcement of the processes of evolution versus involution as affected by certain xenobiotics, such as abused drugs(alcohol and narcotics). The intraneuronal modifications found in the nuclear and cytoplasmic structures of the NRS could possibly reflect the molecular dysfunction related to the production of various types of RNA and neuronal proteins. This hypothesis is supported by biochemical data obtained from analysis of the brain of aged animals. Ultrastructural and biochemical data appear to be in good agreement with the neurophysiologic interpretation of a slow-down and reduced efficiency of the CNS during the progressive development of senescence in human and animal subjects.", "contents": "Neuronal involution during ageing. Ultrastructural study in the rat cerebellum. Involutive phenomena have been investigated by electron microscopy in the Purkinje Pk neuron of the cerebellar cortex of the aging rat. The still limited number of specimens available to date, however, suggest an age-related progression of morphological and functional deteriorations involving particularly the intraneuronal \"nucleus-ribosome system\" (NRS). The impairments are characterized by changes in the nucleolar texture. These alterations are accompanied by modifications in the repartition and relative proportion of RNP components of the nucleolus. In addition, other nuclear elements such as interchromatin and perichromatin granules may vary in importance with age. Recognizable changes in the ribosomal constituents of the NRS are evidenced by modifications in the density and distribution of free ribosomes. An altered structure and organization of GER cisternae are also evident. Furthermore, \"light\" cytoplasmic areas, an increased evidence of neurotubules and the gradual congestion of the pericaryon by age pigments are other valuable ultrastructural features that may be regarded as part of the sequence of morphologic events occurring during neuonal ageing. The above ultrastructural data will subsequently form the basis of a model of ageing in the nerve cell, which will complete the previously proposed model of neuronal maturation. Therefore, this long-term study essentially purports the investigation of subcellular events taking place in the Pk neuron all along the normal life span in rats. This model will also be used to evaluate the changes in the sequence and the reinforcement of the processes of evolution versus involution as affected by certain xenobiotics, such as abused drugs(alcohol and narcotics). The intraneuronal modifications found in the nuclear and cytoplasmic structures of the NRS could possibly reflect the molecular dysfunction related to the production of various types of RNA and neuronal proteins. This hypothesis is supported by biochemical data obtained from analysis of the brain of aged animals. Ultrastructural and biochemical data appear to be in good agreement with the neurophysiologic interpretation of a slow-down and reduced efficiency of the CNS during the progressive development of senescence in human and animal subjects."}
{"id": "PMID:480995", "title": "Age-dependent gene induction in quail oviduct. IX. Age-dependent alterations of DNA-bound amino acids.", "content": "DNA-amino acids (and DNA-protein) complexes were isolated from oviducts of immature, mature and senescent quails by a procedure which does not involve proteolytic enzymes. The content of DNA-amino acids in the complexes, isolated from animals belonging to three different age classes is almost identical and it amounts to about 10 micrograms/mg DNA. However, the fraction of DNA-amino acids which is resistant to pronase is twice as high in lysates from senescent quails as in those from immature and mature animals. The amount of DNA-bound amino acids is strongly dependent on hormone-induced physiological effects in immature quails. If immature animals were treated with estrogen hormone, which causes in induction both of DNA and RNA synthesis, the level of DNA-bound amino acids increases by 220%. This material has been found to be pronase sensitive which might indicate that the amino acids are present as peptides. Treatment of estrogen-stimulated animals with progesterone or inhibitors of DNA and RNA synthesis antagonizes the estrogen-envoked formation of DNA-associated amino acids. From the data we propose that the amount of proteins and/or amino acids, bound to DNA,is not only age-related but also correlated with the physiological state of DNA in immature and mature animals.", "contents": "Age-dependent gene induction in quail oviduct. IX. Age-dependent alterations of DNA-bound amino acids. DNA-amino acids (and DNA-protein) complexes were isolated from oviducts of immature, mature and senescent quails by a procedure which does not involve proteolytic enzymes. The content of DNA-amino acids in the complexes, isolated from animals belonging to three different age classes is almost identical and it amounts to about 10 micrograms/mg DNA. However, the fraction of DNA-amino acids which is resistant to pronase is twice as high in lysates from senescent quails as in those from immature and mature animals. The amount of DNA-bound amino acids is strongly dependent on hormone-induced physiological effects in immature quails. If immature animals were treated with estrogen hormone, which causes in induction both of DNA and RNA synthesis, the level of DNA-bound amino acids increases by 220%. This material has been found to be pronase sensitive which might indicate that the amino acids are present as peptides. Treatment of estrogen-stimulated animals with progesterone or inhibitors of DNA and RNA synthesis antagonizes the estrogen-envoked formation of DNA-associated amino acids. From the data we propose that the amount of proteins and/or amino acids, bound to DNA,is not only age-related but also correlated with the physiological state of DNA in immature and mature animals."}
{"id": "PMID:480996", "title": "Influence of diet on chromatin organization in insect mid-gut cells, and its implications for the understanding of aging in this model system.", "content": "Female Sarcophaga bullata were fed on three different dietary combinations: liver/water; sugar/water; and sugar/liver/water. These diets resulted in different survival patterns for the insect, with the longest lifespans being expressed on the last treatment. The Feulgen-DNA values for mid-gut cell nuclei were determined using a scanning and integrating microdensitometer. The cell nuclei of the mid-gut reacted to the presence of nutrients by an increase in the extinction values for Feulgen-DNA when compared to the unfed state. There were also significant differences between the various treatments in the extent of the activation shown by the mid-gut cells. The dietary regimes resulting in the longest lifespan showed a complete and uniform activation of the mid-gut epithelium, whereas other treatments did not. These observations suggest that changes in the chromatin organization occur with diet, and that maximum Feulgen-DNA extinctions occur (i.e. chromatin uncoiled and supporting transcription) on the sugar/liver/water diet. Nuclei showing maximum extinctions do not show an age-related change between 3 and 12 days of life. However, if the nuclear activation is partial, or incomplete, then age-related reversals in chromatin condensation take place.", "contents": "Influence of diet on chromatin organization in insect mid-gut cells, and its implications for the understanding of aging in this model system. Female Sarcophaga bullata were fed on three different dietary combinations: liver/water; sugar/water; and sugar/liver/water. These diets resulted in different survival patterns for the insect, with the longest lifespans being expressed on the last treatment. The Feulgen-DNA values for mid-gut cell nuclei were determined using a scanning and integrating microdensitometer. The cell nuclei of the mid-gut reacted to the presence of nutrients by an increase in the extinction values for Feulgen-DNA when compared to the unfed state. There were also significant differences between the various treatments in the extent of the activation shown by the mid-gut cells. The dietary regimes resulting in the longest lifespan showed a complete and uniform activation of the mid-gut epithelium, whereas other treatments did not. These observations suggest that changes in the chromatin organization occur with diet, and that maximum Feulgen-DNA extinctions occur (i.e. chromatin uncoiled and supporting transcription) on the sugar/liver/water diet. Nuclei showing maximum extinctions do not show an age-related change between 3 and 12 days of life. However, if the nuclear activation is partial, or incomplete, then age-related reversals in chromatin condensation take place."}
{"id": "PMID:480998", "title": "Measuring components of children's health status.", "content": "Measures of physical, mental and social components of health status and general health ratings were studied for children ages 0-4 (N = 679) and 5-13 (N = 1473). Questionnaires were completed by adult proxies (usually mothers) in three generally healthy populations. Hypothesized multi-item scales were tested; reliability was estimated and preliminary attempts at validation were undertaken. Items in ten scales pertaining to mental health (Anxiety, Depression, Positive Well-Being, Mental Health Index), social health (Social Relations), general health ratings (Current Health, Prior Health, Resistance/Susceptibility to Illness, General Health Rating Index), as well as parental satisfaction with child development satisfied Likert-type and discriminant validity criteria. Because functional limitation items were endorsed for very few children, scales to measure physical health could not be tested. Almost all scales were sufficiently reliable for group comparisons; reliability coefficients were lower in the most disadvantaged population. Interrelationships among scales and validity variables generally supported their construct validity and supported a multi-component model of children's health status.", "contents": "Measuring components of children's health status. Measures of physical, mental and social components of health status and general health ratings were studied for children ages 0-4 (N = 679) and 5-13 (N = 1473). Questionnaires were completed by adult proxies (usually mothers) in three generally healthy populations. Hypothesized multi-item scales were tested; reliability was estimated and preliminary attempts at validation were undertaken. Items in ten scales pertaining to mental health (Anxiety, Depression, Positive Well-Being, Mental Health Index), social health (Social Relations), general health ratings (Current Health, Prior Health, Resistance/Susceptibility to Illness, General Health Rating Index), as well as parental satisfaction with child development satisfied Likert-type and discriminant validity criteria. Because functional limitation items were endorsed for very few children, scales to measure physical health could not be tested. Almost all scales were sufficiently reliable for group comparisons; reliability coefficients were lower in the most disadvantaged population. Interrelationships among scales and validity variables generally supported their construct validity and supported a multi-component model of children's health status."}
{"id": "PMID:480999", "title": "The effect of length of membership upon the utilization of ambulatory care services. A comparison of disadvantaged and general membership populations in a prepaid group practice.", "content": "The relationships between duration of membership and the use of outpatient medical care services for disadvantaged and general membership groups in a prepaid group practice were analyzed. The effects of duration of membership upon utilization rates during a six-year study period were estimated by retrospective cohort analyses. Rates were computed for successive three-month intervals. There were no significant start-up effects on use for new enrollees in either study population. This finding is in contrast to what has been reported in other studies. New HMOs or existing HMOs with many new members may not require the additional resources previously thought necessary. Average cohort utilization rates were higher for the disadvantaged cohorts, with the exception of young males 0 to 14 years old. We also examined the persistence of utilization patterns over time. Consistently high users and nonusers were found in both populations. In general, these findings suggest that length of time does not affect ambulatory care use. Age, sex, socioeconomic background and health status are more important than duration of membership in accounting for differences in utilization.", "contents": "The effect of length of membership upon the utilization of ambulatory care services. A comparison of disadvantaged and general membership populations in a prepaid group practice. The relationships between duration of membership and the use of outpatient medical care services for disadvantaged and general membership groups in a prepaid group practice were analyzed. The effects of duration of membership upon utilization rates during a six-year study period were estimated by retrospective cohort analyses. Rates were computed for successive three-month intervals. There were no significant start-up effects on use for new enrollees in either study population. This finding is in contrast to what has been reported in other studies. New HMOs or existing HMOs with many new members may not require the additional resources previously thought necessary. Average cohort utilization rates were higher for the disadvantaged cohorts, with the exception of young males 0 to 14 years old. We also examined the persistence of utilization patterns over time. Consistently high users and nonusers were found in both populations. In general, these findings suggest that length of time does not affect ambulatory care use. Age, sex, socioeconomic background and health status are more important than duration of membership in accounting for differences in utilization."}
{"id": "PMID:481000", "title": "The relationship between utilization of mental health and somatic health services among low income enrolees in two provider plans.", "content": "Mental health services were included in comprehensive benefits available with no out-of-pocket expenses to enrollees in the Seattle Prepaid Health Care Project. This study was designed to examine the characteristics of users as compared to nonusers of mental health services and to examine the possibility of lower use of somatic health services attributable to the availability of mental health services. Two enrollee groups were studied: one group included enrollees with at least one mental health service (MH-U) and the other included those with some somatic utilization but without mental health utilization (MH-NU). Results indicated that mental health users were different from nonusers based on sociodemographic, health status, and prior utilization measures. Further, the mental health utilizers consumed more somatic services than other enrollees, even controlling for background variables. The visit and admission rates for the MH-U group were 2.4 times that of the MH-NU group, and total inpatient and outpatient costs were three times as high. On all three comparisons, approximately 60 per cent of the difference was accounted for by mental health utilization and by differences in sociodemographic and health status characteristics. The remaining 40 per cent could not be explained, but there is a suggestion that the higher utilization occurred for conditions where medical care is discretionary.", "contents": "The relationship between utilization of mental health and somatic health services among low income enrolees in two provider plans. Mental health services were included in comprehensive benefits available with no out-of-pocket expenses to enrollees in the Seattle Prepaid Health Care Project. This study was designed to examine the characteristics of users as compared to nonusers of mental health services and to examine the possibility of lower use of somatic health services attributable to the availability of mental health services. Two enrollee groups were studied: one group included enrollees with at least one mental health service (MH-U) and the other included those with some somatic utilization but without mental health utilization (MH-NU). Results indicated that mental health users were different from nonusers based on sociodemographic, health status, and prior utilization measures. Further, the mental health utilizers consumed more somatic services than other enrollees, even controlling for background variables. The visit and admission rates for the MH-U group were 2.4 times that of the MH-NU group, and total inpatient and outpatient costs were three times as high. On all three comparisons, approximately 60 per cent of the difference was accounted for by mental health utilization and by differences in sociodemographic and health status characteristics. The remaining 40 per cent could not be explained, but there is a suggestion that the higher utilization occurred for conditions where medical care is discretionary."}
{"id": "PMID:481001", "title": "The effect of delay rules in controlling unscheduled visits to hospitals.", "content": "The increased demand of unscheduled visits to hospital outpatient facilities, and particularly to walk-in clinics, necessitates the consideration of means to control this flow and to reduce in-hospital waiting times. A model is developed in which a percentage of the unscheduled visits are assumed to be delayable, e.g., patients with non-urgent complaints who call may be asked to delay their arrivals for specified lengths of time. A simple rule for determining, dynamically, the length of this delay was examined by computer simulation. The results demonstrate significant reduction of in-facility waiting times while only marginally increasing the time patients wait from their initial contact with the clinic until seen by a practitioner.", "contents": "The effect of delay rules in controlling unscheduled visits to hospitals. The increased demand of unscheduled visits to hospital outpatient facilities, and particularly to walk-in clinics, necessitates the consideration of means to control this flow and to reduce in-hospital waiting times. A model is developed in which a percentage of the unscheduled visits are assumed to be delayable, e.g., patients with non-urgent complaints who call may be asked to delay their arrivals for specified lengths of time. A simple rule for determining, dynamically, the length of this delay was examined by computer simulation. The results demonstrate significant reduction of in-facility waiting times while only marginally increasing the time patients wait from their initial contact with the clinic until seen by a practitioner."}
{"id": "PMID:481002", "title": "[Classification of the disease and pathologic type of bronchogenic carcinoma (III) (author's transl)].", "content": "The classification of bronchogenic carcinoma according to its extension and evolutive stages is useful in order to be able to organize epidemiologic and therapeutic studies, as well for exchanging information. At the present time, however, it is very difficult to establish a common classification for all of the groups working on bronchogenic carcinoma. The Bronchogenic Carcinoma Cooperative Group classified the cases following parameters such as operability, resectability, localized or generalized disease, and do not completely agree with the value of the TNM classifications. In the same way in respect to the different pathologic type of bronchogenic carcinoma, the traditional decision not to operate that follows the presentation of the undifferentiated types comes under discussion. This is due to the wide diagnostic variability in the evaluation of the different pathologic types according to the experience of various pneumopathologists published in the literature.", "contents": "[Classification of the disease and pathologic type of bronchogenic carcinoma (III) (author's transl)]. The classification of bronchogenic carcinoma according to its extension and evolutive stages is useful in order to be able to organize epidemiologic and therapeutic studies, as well for exchanging information. At the present time, however, it is very difficult to establish a common classification for all of the groups working on bronchogenic carcinoma. The Bronchogenic Carcinoma Cooperative Group classified the cases following parameters such as operability, resectability, localized or generalized disease, and do not completely agree with the value of the TNM classifications. In the same way in respect to the different pathologic type of bronchogenic carcinoma, the traditional decision not to operate that follows the presentation of the undifferentiated types comes under discussion. This is due to the wide diagnostic variability in the evaluation of the different pathologic types according to the experience of various pneumopathologists published in the literature."}
{"id": "PMID:481006", "title": "[Pulmonary nocardiosis. A case report (author's transl)].", "content": "Within the genus Nocardia three species are at present considered to have human pathologic interest: N. asteroides, N. brasiliensis, and N. caviae. These species are usually the etiologic agents of at least two clinical pictures: nocardiosis and actinomycetoma. A case of pulmonar nocardiosis in a 62-year-old male is reported. The patient had asthmatic episodes and on several occasions received treatment with corticoids. The clinical picture basically consisted of an impairment of the general condition with respiratory symptomatology and fever of 38 to 39 degrees C. An opaque pleuropulmonary image was observed on the chest X-ray film, and the diagnosis of tuberculosis was initially established. The correct diagnosis was confirmed through the bacteriological examination of the transtracheal aspirate with the isolation of N. asteroides. Treatment with streptomycin, sulfamethoxazole and trimethoprim achieved the clinical and roentgenologic cure of the patient. After 2 months of treatment the clinical manifestations had disappeared and after 5 months the chest X-ray was normal. Some epidemiologic data are reviewed.", "contents": "[Pulmonary nocardiosis. A case report (author's transl)]. Within the genus Nocardia three species are at present considered to have human pathologic interest: N. asteroides, N. brasiliensis, and N. caviae. These species are usually the etiologic agents of at least two clinical pictures: nocardiosis and actinomycetoma. A case of pulmonar nocardiosis in a 62-year-old male is reported. The patient had asthmatic episodes and on several occasions received treatment with corticoids. The clinical picture basically consisted of an impairment of the general condition with respiratory symptomatology and fever of 38 to 39 degrees C. An opaque pleuropulmonary image was observed on the chest X-ray film, and the diagnosis of tuberculosis was initially established. The correct diagnosis was confirmed through the bacteriological examination of the transtracheal aspirate with the isolation of N. asteroides. Treatment with streptomycin, sulfamethoxazole and trimethoprim achieved the clinical and roentgenologic cure of the patient. After 2 months of treatment the clinical manifestations had disappeared and after 5 months the chest X-ray was normal. Some epidemiologic data are reviewed."}
{"id": "PMID:481007", "title": "[Hormonal contraceptives and high blood pressure (author's transl)].", "content": "The aim of this report is to offer an explanation for the high incidence of arterial hypertension in women taken hormone contraceptives. The real incidence of this association has been considered in women initially having a normal blood pressure and in others who had high blood pressure before using these contraceptives. The estrogen and progestogen components in hormone contraceptives were analyzed individually in various studies. The most recent investigations seem to indicate that progestogen is the main cause of high blood pressure. Different mechanism that could link hormone contraceptives to high blood pressure were investigated. The renin-angiotensin-aldosterone axis involving the action of estrogens and progestogens on the renin substrate, plasma renin, angiotensin II and aldosterone were analyzed. Another possible mechanism could involve glucocorticoids, altering the metabolism of glucose, pyruvate, cholesterol, and triglycerides, Kidney disease involving renal function, microangiopathic anemia, and renal thromboembolism; hyperactivity of the sympathetic nervous system (noraepinephrine and dopamine-beta-hidroxylase blood levels); prostaglandins; genetic mechanism; and individual mechanism were all taken into consideration. Lastly the priorities of the different systems linking high blood pressure to hormone contraceptives and the relationships between them are analyzed.", "contents": "[Hormonal contraceptives and high blood pressure (author's transl)]. The aim of this report is to offer an explanation for the high incidence of arterial hypertension in women taken hormone contraceptives. The real incidence of this association has been considered in women initially having a normal blood pressure and in others who had high blood pressure before using these contraceptives. The estrogen and progestogen components in hormone contraceptives were analyzed individually in various studies. The most recent investigations seem to indicate that progestogen is the main cause of high blood pressure. Different mechanism that could link hormone contraceptives to high blood pressure were investigated. The renin-angiotensin-aldosterone axis involving the action of estrogens and progestogens on the renin substrate, plasma renin, angiotensin II and aldosterone were analyzed. Another possible mechanism could involve glucocorticoids, altering the metabolism of glucose, pyruvate, cholesterol, and triglycerides, Kidney disease involving renal function, microangiopathic anemia, and renal thromboembolism; hyperactivity of the sympathetic nervous system (noraepinephrine and dopamine-beta-hidroxylase blood levels); prostaglandins; genetic mechanism; and individual mechanism were all taken into consideration. Lastly the priorities of the different systems linking high blood pressure to hormone contraceptives and the relationships between them are analyzed."}
{"id": "PMID:481009", "title": "[Plasma B12 vitamin and folic acid during chronic renal failure and hemodialysis (author's transl)].", "content": "Thirty-one patients suffering from very advanced degrees of chronic renal insufficiency were studied. Ten received a hypoproteic diet as the only treatment and the remaining 21 were on a program of hemodialysis with a free diet and no vitamin supplements. Basal and pre- and post-dialysis plasma determinations of B12 vitamin and folic acid were carried out on them. Looking at the results it was verified that there was a significant reduction in the plasma values of both B12 vitamin and folic acid in the cases of low protein intake. In dialysis we saw that although folic acid was lost during sessions the free dietetic intake was enough to compensate for these losses. As for B12 vitamin, although it is described as dialyzable, we did not observe that its post-dialysis values disminished. Our conclusions indicate that a supplementary vitamin contribution is not necessary for patients who are on a program of hemodialysis.", "contents": "[Plasma B12 vitamin and folic acid during chronic renal failure and hemodialysis (author's transl)]. Thirty-one patients suffering from very advanced degrees of chronic renal insufficiency were studied. Ten received a hypoproteic diet as the only treatment and the remaining 21 were on a program of hemodialysis with a free diet and no vitamin supplements. Basal and pre- and post-dialysis plasma determinations of B12 vitamin and folic acid were carried out on them. Looking at the results it was verified that there was a significant reduction in the plasma values of both B12 vitamin and folic acid in the cases of low protein intake. In dialysis we saw that although folic acid was lost during sessions the free dietetic intake was enough to compensate for these losses. As for B12 vitamin, although it is described as dialyzable, we did not observe that its post-dialysis values disminished. Our conclusions indicate that a supplementary vitamin contribution is not necessary for patients who are on a program of hemodialysis."}
{"id": "PMID:481010", "title": "[Association of VCR-5FU-CYCLO-PDN in the treatment of disseminated carcinoma of the breast (author's transl)].", "content": "A group of 32 patients with disseminated breast cancer were submitted to polychemotherapy following Cooper's protocol. Vincristine, cyclophosphamide, 5-fluorouracil, and prednisone were administered. The fifth drug used by Cooper, methotrexate, was not included in the therapy program because of difficulties in obtaining it. Both the objective and subjective results were similar to those of other authors using similar drug associations, while toxicity was moderate. Although this treatment is effective to some degree, the authors feel that further research should be carried out on more potent drugs, or in different combinations, in order to get a more definite chemotherapeutic effect in patients with breast cancer.", "contents": "[Association of VCR-5FU-CYCLO-PDN in the treatment of disseminated carcinoma of the breast (author's transl)]. A group of 32 patients with disseminated breast cancer were submitted to polychemotherapy following Cooper's protocol. Vincristine, cyclophosphamide, 5-fluorouracil, and prednisone were administered. The fifth drug used by Cooper, methotrexate, was not included in the therapy program because of difficulties in obtaining it. Both the objective and subjective results were similar to those of other authors using similar drug associations, while toxicity was moderate. Although this treatment is effective to some degree, the authors feel that further research should be carried out on more potent drugs, or in different combinations, in order to get a more definite chemotherapeutic effect in patients with breast cancer."}
{"id": "PMID:481011", "title": "[A new pathology: unstable urinary bladder (author's transl)].", "content": "Thirty-five cases of incontinence of urine due to hyperactivity of the detrusor as a result of unstable urinary bladder were found among 100 patients with incontinence. The cause of the hyperactivity was established after a thorough urological and neurological screening with intravenous urography, seriated mictional cystoureterography, cystoscopy, and urine culture. The diagnosis was considered definite if uninhibited contractions of the detrusor were detected by electrocystomanometry. The primary clinical symptom was an imperious urge to mi-turate, with or without leakage of urine. Diurnal and nocturnal frequency was exacerbated in cold weather and in contact with water. There were some instances of a history of late enuresis and association with cystoceles. Anticholinergics (propantheline bromide) are the treatment of choice. Surgery should be indicated only after all medical attempts have failed.", "contents": "[A new pathology: unstable urinary bladder (author's transl)]. Thirty-five cases of incontinence of urine due to hyperactivity of the detrusor as a result of unstable urinary bladder were found among 100 patients with incontinence. The cause of the hyperactivity was established after a thorough urological and neurological screening with intravenous urography, seriated mictional cystoureterography, cystoscopy, and urine culture. The diagnosis was considered definite if uninhibited contractions of the detrusor were detected by electrocystomanometry. The primary clinical symptom was an imperious urge to mi-turate, with or without leakage of urine. Diurnal and nocturnal frequency was exacerbated in cold weather and in contact with water. There were some instances of a history of late enuresis and association with cystoceles. Anticholinergics (propantheline bromide) are the treatment of choice. Surgery should be indicated only after all medical attempts have failed."}
{"id": "PMID:481012", "title": "[Pulmonary function differences in healthy subjects according to postural changes (author's transl)].", "content": "The total lung capacity (TLC) and its subdivisions along with the forced spirometric values (FEF25-75 and FEV1) were determined in a group of 44 healthy subjects, composed of 29 women and 15 men. The determinations were carried out in a sitting position and in supine position, breathing air. All the pulmonary volumes diminished when the subject lay down, but the functional residual capacity (FRC) was that which underwent a greater reduction. The residual volume decrease was the only one which did not reach statistically significant levels. Women always showed smaller pulmonary volumes than men. It has been demonstrated that FRC can be exactly measured by means of the closed helium technique. The regression figures of FRC in supine position were determinted for both sexes, with a r = 0,33 (p less than 0,1) in women and r = 0,95 (p less than 0.001) in men. The forced spirometric tests did not undergo any important changes although the reduction of the FEV1 was significant in both sexes. The results obtained were compared with those published by other authors.", "contents": "[Pulmonary function differences in healthy subjects according to postural changes (author's transl)]. The total lung capacity (TLC) and its subdivisions along with the forced spirometric values (FEF25-75 and FEV1) were determined in a group of 44 healthy subjects, composed of 29 women and 15 men. The determinations were carried out in a sitting position and in supine position, breathing air. All the pulmonary volumes diminished when the subject lay down, but the functional residual capacity (FRC) was that which underwent a greater reduction. The residual volume decrease was the only one which did not reach statistically significant levels. Women always showed smaller pulmonary volumes than men. It has been demonstrated that FRC can be exactly measured by means of the closed helium technique. The regression figures of FRC in supine position were determinted for both sexes, with a r = 0,33 (p less than 0,1) in women and r = 0,95 (p less than 0.001) in men. The forced spirometric tests did not undergo any important changes although the reduction of the FEV1 was significant in both sexes. The results obtained were compared with those published by other authors."}
{"id": "PMID:481029", "title": "[Aspiration biopsy cytology of the diseases of the mammary gland (author's transl)].", "content": "A detailed report is given on the results of aspiration cytology used in 612 patients. On the basis of his own experiences he speaks high of cytodiagnostics in the complex of cyto-radio-clinical triplet, where its value may reach 98-100% to the contrary of 60-88% of the other two components. It is rapid, trustful, undangerous, in everyday practice is suggested at every place, where the personal and objective conditions are guaranteed.", "contents": "[Aspiration biopsy cytology of the diseases of the mammary gland (author's transl)]. A detailed report is given on the results of aspiration cytology used in 612 patients. On the basis of his own experiences he speaks high of cytodiagnostics in the complex of cyto-radio-clinical triplet, where its value may reach 98-100% to the contrary of 60-88% of the other two components. It is rapid, trustful, undangerous, in everyday practice is suggested at every place, where the personal and objective conditions are guaranteed."}
{"id": "PMID:481030", "title": "[Critical study on dead cases in recurrent early stomach cancer (author's transl)].", "content": "35 deaths of 299 operated early stomach cancers have been studied retrospectively. These cases can be grouped in the following categories: 2 deaths directly due to operation, 7 due to recurrence of carcinoma, 19 due to other diseases including cancer in other sites and 7 cases in which the real causes of death could not be elucidated. Special attention was paid on the 7 recurrent cases: In 4 of 7 patients the histological study revealed lymph and blood vessels invasion in their resected stomachs, lymphnodes metastases in 5 patients and positive cut-end in 2 patients. 5 of 7 patients died within 3 years after operation.", "contents": "[Critical study on dead cases in recurrent early stomach cancer (author's transl)]. 35 deaths of 299 operated early stomach cancers have been studied retrospectively. These cases can be grouped in the following categories: 2 deaths directly due to operation, 7 due to recurrence of carcinoma, 19 due to other diseases including cancer in other sites and 7 cases in which the real causes of death could not be elucidated. Special attention was paid on the 7 recurrent cases: In 4 of 7 patients the histological study revealed lymph and blood vessels invasion in their resected stomachs, lymphnodes metastases in 5 patients and positive cut-end in 2 patients. 5 of 7 patients died within 3 years after operation."}
{"id": "PMID:481032", "title": "[Resection of the valvula bauhini and its effect on the metabolism. An experimental study in animals (author's transl)].", "content": "In animal experiments (20 mongrel dogs) malabsorption was induced by resection of 100 cm ileum. Subsequently the influence of the ileocoecal valve (Valvula Bauhini) in absorption was studied. In one group of animals intestinal continuity was reestablished by ileoileostomy, in a second by ileocolostomy. The results of the absorption studies were compared with a healthy control group. After ileoileostomy animals do not lose weight, serum cholesterol and protein remain normal. Oral tolerance tests with Triolein-iodine-131 and Cyanocobalamine show results identical with those of the control group. After ileocolostomy all these parameters are lowered--partially to a pathological level. It is concluded that in all operations in the area of the terminal ileum and colon the Valvula Bauhini should be retained if possible.", "contents": "[Resection of the valvula bauhini and its effect on the metabolism. An experimental study in animals (author's transl)]. In animal experiments (20 mongrel dogs) malabsorption was induced by resection of 100 cm ileum. Subsequently the influence of the ileocoecal valve (Valvula Bauhini) in absorption was studied. In one group of animals intestinal continuity was reestablished by ileoileostomy, in a second by ileocolostomy. The results of the absorption studies were compared with a healthy control group. After ileoileostomy animals do not lose weight, serum cholesterol and protein remain normal. Oral tolerance tests with Triolein-iodine-131 and Cyanocobalamine show results identical with those of the control group. After ileocolostomy all these parameters are lowered--partially to a pathological level. It is concluded that in all operations in the area of the terminal ileum and colon the Valvula Bauhini should be retained if possible."}
{"id": "PMID:481033", "title": "[The oxygen tension in the gastric fundus wall in relation to arterial blood supply. Experimental study with intramural pO2-measurement (author's transl)].", "content": "Animal experiments in dogs were performed in order to measure oxygen tension in the gastric fundus. For this purpose an intravasal oxygen catheter electrode was inserted into the gastric submucosa. The probe recorded pO2 in an area about 2 cm in length. The oxygen tension was measured in relation to the respective blood supply of stomach. The method is suitable for determining the tendency of oxygen supply of the tissue intraoperatively as well as after implantation postoperatively. The clinical interpretation of the trend of pO2-measurements depends on further experiences.", "contents": "[The oxygen tension in the gastric fundus wall in relation to arterial blood supply. Experimental study with intramural pO2-measurement (author's transl)]. Animal experiments in dogs were performed in order to measure oxygen tension in the gastric fundus. For this purpose an intravasal oxygen catheter electrode was inserted into the gastric submucosa. The probe recorded pO2 in an area about 2 cm in length. The oxygen tension was measured in relation to the respective blood supply of stomach. The method is suitable for determining the tendency of oxygen supply of the tissue intraoperatively as well as after implantation postoperatively. The clinical interpretation of the trend of pO2-measurements depends on further experiences."}
{"id": "PMID:481034", "title": "[Paravasal sclerotherapy: experimental study on the effect of sclerosing agents (author's transl)].", "content": "Sclerosing and side effects of three solutions mostly used for paravasal sclerotherapy were studied by subcutaneous injections in lower legs of rats. The results demonstrate that of the three examined sclerosing agents 5 p.c. phenol in almond oil has the lowest toxocity in tissue besides a sufficient production of collagen fibres.", "contents": "[Paravasal sclerotherapy: experimental study on the effect of sclerosing agents (author's transl)]. Sclerosing and side effects of three solutions mostly used for paravasal sclerotherapy were studied by subcutaneous injections in lower legs of rats. The results demonstrate that of the three examined sclerosing agents 5 p.c. phenol in almond oil has the lowest toxocity in tissue besides a sufficient production of collagen fibres."}
{"id": "PMID:481035", "title": "[On microsurgical treatment of vocal cord carcinomas with CO2-laser (author's transl)].", "content": "The follow up of a few patients with cancer of the vocal cords treated with CO2-Laser microsurgery is described. This new technique seems to be suitable not only in the treatment of benign lesions of the larynx, but also for malignant tumors of determined localisation and extension. Owing to a relatively short observation time a final judgment of the recurrence rate is not yet possible.", "contents": "[On microsurgical treatment of vocal cord carcinomas with CO2-laser (author's transl)]. The follow up of a few patients with cancer of the vocal cords treated with CO2-Laser microsurgery is described. This new technique seems to be suitable not only in the treatment of benign lesions of the larynx, but also for malignant tumors of determined localisation and extension. Owing to a relatively short observation time a final judgment of the recurrence rate is not yet possible."}
{"id": "PMID:481036", "title": "[Five-year-cure rate of laryngeal cancer in the Kiel clinic. Report of 216 cases from 1960 to 1967 (then director: Prof. Dr. E. M\u00fcller) with special reference to indications for surgical or orthovolt x-ray therapy].", "content": "Report about the five-years-cure of all cases of carcinoma of the larynx, the Kiel-University-ORL-Department had to engage in with diagnostical and therapeutical treatment within a period of 7 years. Out of the total of 216 patients the absolute survival rate was 144 (= 66,7%) after five years. Cure rate was the best with carcinoma of the true vocal cord (72,8%), less satisfactory with carcinoma of the supraglottic region (61,1%), only 5/14 with carcinoma of the marginal region, and only 2/8 with advanced and widespread tumours of undefinable origin. After comparing the distribution of the total of our patients with those of other authors, our system of diagnosis, staging, and classification is described in detail being the basis for a skilful choice of primary surgical therapy on the one hand or primary irradiation on the other hand (in our cases orthovolt-X-ray). Both the primary treatment and treatment of recurrence-cases are described in detail, differing from descriptions and claims of other authors for \"modern\" therapy in many items. Nevertheless we have comparatively very good results, they demand a critical standpoint towards new therapeutical methods. In our opinion close collaboration between laryngologists and radiotherapists concerning classification of tumours, staging and choice of therapy, treatment and post-clinical observation is the fundamental condition for obtaining good and better results in treatment of laryngeal carcinoma.", "contents": "[Five-year-cure rate of laryngeal cancer in the Kiel clinic. Report of 216 cases from 1960 to 1967 (then director: Prof. Dr. E. M\u00fcller) with special reference to indications for surgical or orthovolt x-ray therapy]. Report about the five-years-cure of all cases of carcinoma of the larynx, the Kiel-University-ORL-Department had to engage in with diagnostical and therapeutical treatment within a period of 7 years. Out of the total of 216 patients the absolute survival rate was 144 (= 66,7%) after five years. Cure rate was the best with carcinoma of the true vocal cord (72,8%), less satisfactory with carcinoma of the supraglottic region (61,1%), only 5/14 with carcinoma of the marginal region, and only 2/8 with advanced and widespread tumours of undefinable origin. After comparing the distribution of the total of our patients with those of other authors, our system of diagnosis, staging, and classification is described in detail being the basis for a skilful choice of primary surgical therapy on the one hand or primary irradiation on the other hand (in our cases orthovolt-X-ray). Both the primary treatment and treatment of recurrence-cases are described in detail, differing from descriptions and claims of other authors for \"modern\" therapy in many items. Nevertheless we have comparatively very good results, they demand a critical standpoint towards new therapeutical methods. In our opinion close collaboration between laryngologists and radiotherapists concerning classification of tumours, staging and choice of therapy, treatment and post-clinical observation is the fundamental condition for obtaining good and better results in treatment of laryngeal carcinoma."}
{"id": "PMID:481037", "title": "[Some reasons for failure in tympanoplasty (author's transl)].", "content": "The reasons for failures in tympanoplasty are classified and discussed as anatomical or pathological ones and those, that rise from method and technique. A main cause insists in the pathopolymorphism of the chronic purulent otitis media and the discrepancy of clinical impression and pathological reality. The author wishes to encourage the surgeons to a more radical technique by these examples and to the use of new methods in the field of reconstructive surgery of the middle ear.", "contents": "[Some reasons for failure in tympanoplasty (author's transl)]. The reasons for failures in tympanoplasty are classified and discussed as anatomical or pathological ones and those, that rise from method and technique. A main cause insists in the pathopolymorphism of the chronic purulent otitis media and the discrepancy of clinical impression and pathological reality. The author wishes to encourage the surgeons to a more radical technique by these examples and to the use of new methods in the field of reconstructive surgery of the middle ear."}
{"id": "PMID:481038", "title": "[Investigation of the problem of safety of computer models in automatic analysis of nystagmus (author's transl)].", "content": "The aim of the investigations was to demonstrate by means of statistical methods, that there was no signifikant difference between manual calculation and automatical computer analysis of ENG.", "contents": "[Investigation of the problem of safety of computer models in automatic analysis of nystagmus (author's transl)]. The aim of the investigations was to demonstrate by means of statistical methods, that there was no signifikant difference between manual calculation and automatical computer analysis of ENG."}
{"id": "PMID:481039", "title": "[Slow cortical evoked potentials after noise exposure].", "content": "Human cortical evoked potentials under conditions of stimulation are registrated in the post-stimulatory phase of a five minutes lasting equally masking white noise (90 dB HL). Changes of the evoked potentials during to adaptation, possible analogy with high tone losses after noise representation and the origin of tinnitus are examined. Stimulation was started 3 sec after the off-effect of the noise. For five minutes periodically tone bursts were represented. Each train of stimulation consists of tone bursts of three frequencies: 2 kcs, 4 kcs, 8 kcs. The 0.5 sec lasting tones were separated by pauses of 2 sec. During the experiment stimulation and analysis were controlled by a computer. Changes in latency and amplitudes of the cortical evoked potentials were registered. Changes of the adaption patterns as a function of the poststimulatory time are discussed.", "contents": "[Slow cortical evoked potentials after noise exposure]. Human cortical evoked potentials under conditions of stimulation are registrated in the post-stimulatory phase of a five minutes lasting equally masking white noise (90 dB HL). Changes of the evoked potentials during to adaptation, possible analogy with high tone losses after noise representation and the origin of tinnitus are examined. Stimulation was started 3 sec after the off-effect of the noise. For five minutes periodically tone bursts were represented. Each train of stimulation consists of tone bursts of three frequencies: 2 kcs, 4 kcs, 8 kcs. The 0.5 sec lasting tones were separated by pauses of 2 sec. During the experiment stimulation and analysis were controlled by a computer. Changes in latency and amplitudes of the cortical evoked potentials were registered. Changes of the adaption patterns as a function of the poststimulatory time are discussed."}
{"id": "PMID:481040", "title": "[Acoustic evoked potentials in presbycusis].", "content": "Investigations concerning presbyacusis indicate that the ageing process of the hearing organ is mostly influenced by changes in the sensory-neural pathways. Psychoacoustic measurements give only reduced information on the peripheral and central part of the hearing organ. To verify changes of transport processes in the hearing organ caused by age we examined different evoked potentials. Occurrence probability of positive wave-complexes, input-output functions of amplitude and latency and particular changes in response pattern are analyzed. The results are controled by a signal-analyzer-system using the classical t-test. Thus we could prove data of three age groups and of sex-differences. Our examination partically confirm morphological and histological data of reduced transport processes in most regions of the auditory pathays.", "contents": "[Acoustic evoked potentials in presbycusis]. Investigations concerning presbyacusis indicate that the ageing process of the hearing organ is mostly influenced by changes in the sensory-neural pathways. Psychoacoustic measurements give only reduced information on the peripheral and central part of the hearing organ. To verify changes of transport processes in the hearing organ caused by age we examined different evoked potentials. Occurrence probability of positive wave-complexes, input-output functions of amplitude and latency and particular changes in response pattern are analyzed. The results are controled by a signal-analyzer-system using the classical t-test. Thus we could prove data of three age groups and of sex-differences. Our examination partically confirm morphological and histological data of reduced transport processes in most regions of the auditory pathays."}
{"id": "PMID:481041", "title": "[Inhalation therapy of sinusitis with the Breathing Mask 500].", "content": "The subject of this analysis are the results of treatment of sinusitis in its varying forms and degrees with the \"Climate Mask 500\" in conjunction with antrum puncture. Either dry or humit warm-air inhalations with coniferous oil additives were prescribed depending on the type of sillness. In 100% of the cases so treated normalisation or improvement of the subjective complaint could be observed. The objective findings normalised or improved in 80% of the cases. Because of its therapeutic value, its simplicity, the reduction in medication and the lack of side effects, inhalation treatment with the \"Climate Mask 500\" can be said to be a useful aid in a causal therapy of sinusitis.", "contents": "[Inhalation therapy of sinusitis with the Breathing Mask 500]. The subject of this analysis are the results of treatment of sinusitis in its varying forms and degrees with the \"Climate Mask 500\" in conjunction with antrum puncture. Either dry or humit warm-air inhalations with coniferous oil additives were prescribed depending on the type of sillness. In 100% of the cases so treated normalisation or improvement of the subjective complaint could be observed. The objective findings normalised or improved in 80% of the cases. Because of its therapeutic value, its simplicity, the reduction in medication and the lack of side effects, inhalation treatment with the \"Climate Mask 500\" can be said to be a useful aid in a causal therapy of sinusitis."}
{"id": "PMID:481042", "title": "Sudden hearing loss in divers and fliers.", "content": "Many papers have been written about sudden sensory hearing loss and the effect of barotrauma on the inner ear. Fistulae of the round and oval window membranes have been implicated in the great majority of these cases. It has usually been recommended that the patient be treated with conservative therapy, such as bed rest, for a period of as long as 30 days and that the final hearing results are as good or better than those that have been surgically explored and corrected. In our experience immediate surgical exploration and correction of sudden severe or profound sensorineural deafness in the diver or flier is absolutely essential and the excellent results of hearing improvement in this select group certainly corroborates this theory. Other cases with the hearing loss limited to the high frequencies most notably have tinnitus and surgical exploration does not improve the hearing but may improve vertigo if present. Numerous cases are presented to support these supositions.", "contents": "Sudden hearing loss in divers and fliers. Many papers have been written about sudden sensory hearing loss and the effect of barotrauma on the inner ear. Fistulae of the round and oval window membranes have been implicated in the great majority of these cases. It has usually been recommended that the patient be treated with conservative therapy, such as bed rest, for a period of as long as 30 days and that the final hearing results are as good or better than those that have been surgically explored and corrected. In our experience immediate surgical exploration and correction of sudden severe or profound sensorineural deafness in the diver or flier is absolutely essential and the excellent results of hearing improvement in this select group certainly corroborates this theory. Other cases with the hearing loss limited to the high frequencies most notably have tinnitus and surgical exploration does not improve the hearing but may improve vertigo if present. Numerous cases are presented to support these supositions."}
{"id": "PMID:481043", "title": "Pediatric vocal cord paralysis.", "content": "Pediatric vocal cord paralysis accounts for approximately 10% of all congenital laryngeal lesions. Early detection of these neurogenic disorders is based upon a high index of suspicion and is important to prevent catastrophes during periods of acute respiratory embarrassment. This paper reviews the symptoms, etiology and management of unilateral and bilateral vocal cord paralysis as it pertains to this age group. A case is presented of a neonate with vocal cord paralysis and associated hydrocephalous and meningomyelocele to illustrate many of the problems associated with pediatric laryngeal paralysis.", "contents": "Pediatric vocal cord paralysis. Pediatric vocal cord paralysis accounts for approximately 10% of all congenital laryngeal lesions. Early detection of these neurogenic disorders is based upon a high index of suspicion and is important to prevent catastrophes during periods of acute respiratory embarrassment. This paper reviews the symptoms, etiology and management of unilateral and bilateral vocal cord paralysis as it pertains to this age group. A case is presented of a neonate with vocal cord paralysis and associated hydrocephalous and meningomyelocele to illustrate many of the problems associated with pediatric laryngeal paralysis."}
{"id": "PMID:481044", "title": "Percutaneous embolization to control intractable epistaxis.", "content": "Recurrent epistaxis is a common manifestation of patients with a bleeding diathesis. Two patients with epistaxis secondary to a bleeding diathesis managed by local conservative techniques are reviewed. (A case of polycythemia vera and a case of liver failure secondary to hepatoma are reviewed.) Recently bilateral, percutaneous carotid angiography examination was performed on a patient with a bleeding diathesis and intractable epistaxis. At the time of the angiographic examination, embolization of both internal maxillary arteries with Gelfoam particles was accomplished and dramatic control of the epistaxis was achieved. In a patient with severe epistaxis secondary to a bleeding diathesis that is unresponsive to local measures, percutaneous Gelfoam embolization offers substantial advantages over surgical intervention.", "contents": "Percutaneous embolization to control intractable epistaxis. Recurrent epistaxis is a common manifestation of patients with a bleeding diathesis. Two patients with epistaxis secondary to a bleeding diathesis managed by local conservative techniques are reviewed. (A case of polycythemia vera and a case of liver failure secondary to hepatoma are reviewed.) Recently bilateral, percutaneous carotid angiography examination was performed on a patient with a bleeding diathesis and intractable epistaxis. At the time of the angiographic examination, embolization of both internal maxillary arteries with Gelfoam particles was accomplished and dramatic control of the epistaxis was achieved. In a patient with severe epistaxis secondary to a bleeding diathesis that is unresponsive to local measures, percutaneous Gelfoam embolization offers substantial advantages over surgical intervention."}
{"id": "PMID:481045", "title": "Rehabilitation of the face following traumatic injury to the facial nerve.", "content": "Etiology, diagnosis, and surgical management of facial paralysis due to traumatic injury of the VIIth cranial nerve are discussed. Sixty patients are reviewed who underwent some type of surgical procedure for the repair of the facial nerve. These cases are categorized according to etiology, which includes temporal bone fractures, iatrogenic injuries, and penetrating wounds of the head and neck. The results of a poll of eight leading otologists on their approaches to several aspects of the surgical management of these injuries are presented in the Discussion section. The diagnostic and prognostic studies associated with facial paralysis, as well as the more common surgical procedures available for repair of the facial nerve, are briefly reviewed.", "contents": "Rehabilitation of the face following traumatic injury to the facial nerve. Etiology, diagnosis, and surgical management of facial paralysis due to traumatic injury of the VIIth cranial nerve are discussed. Sixty patients are reviewed who underwent some type of surgical procedure for the repair of the facial nerve. These cases are categorized according to etiology, which includes temporal bone fractures, iatrogenic injuries, and penetrating wounds of the head and neck. The results of a poll of eight leading otologists on their approaches to several aspects of the surgical management of these injuries are presented in the Discussion section. The diagnostic and prognostic studies associated with facial paralysis, as well as the more common surgical procedures available for repair of the facial nerve, are briefly reviewed."}
{"id": "PMID:481046", "title": "Schwannomas of the parapharyngeal space and jugular foramen.", "content": "Schwannomas or neurilemmomas are among the most common neoplasms occupying the parapharyngeal space, yet only 107 cases have been previously reported. Neurilemmomas involving the jugular foramen are extremely rare. Only 55 cases have been reported in the world literature. The neoplasm occurred in the parapharyngeal space in three of our patients and in the jugular foramen in another patient. Of the tumors located in the parapharyngeal space, the nerve of origin in one of them was the glossopharyngeal, which is extremely rare. Adequate exposure for complete excision of parapharyngeal space tumors is best obtained through an external incision and should not be attempted transorally. In the jugular foramen case, the neoplasm arose from the vagus nerve high in the neck and extended intracranially in a \"dumbbell\" shape into the posterior cranial fossa. Total removal was successfully accomplished in one stage, by using a subtotal temporal bone resection--upper neck--posterior cranial fossa approach. Surgical removal is the treatment of choice. Schwannomas rarely recur following complete excision.", "contents": "Schwannomas of the parapharyngeal space and jugular foramen. Schwannomas or neurilemmomas are among the most common neoplasms occupying the parapharyngeal space, yet only 107 cases have been previously reported. Neurilemmomas involving the jugular foramen are extremely rare. Only 55 cases have been reported in the world literature. The neoplasm occurred in the parapharyngeal space in three of our patients and in the jugular foramen in another patient. Of the tumors located in the parapharyngeal space, the nerve of origin in one of them was the glossopharyngeal, which is extremely rare. Adequate exposure for complete excision of parapharyngeal space tumors is best obtained through an external incision and should not be attempted transorally. In the jugular foramen case, the neoplasm arose from the vagus nerve high in the neck and extended intracranially in a \"dumbbell\" shape into the posterior cranial fossa. Total removal was successfully accomplished in one stage, by using a subtotal temporal bone resection--upper neck--posterior cranial fossa approach. Surgical removal is the treatment of choice. Schwannomas rarely recur following complete excision."}
{"id": "PMID:481047", "title": "The grand biopsy for the \"cold\" thyroid nodule.", "content": "A lobe and isthmusectomy is our standard surgical management for the cold nodule of the thyroid. Experience indicates that the great majority of cold nodules prove benign. It is manifest that the attendant morbidity for this surgery be extremely low. Our experience reviewing thyroid surgery for only benign disease indicates that complications are indeed uncommon. Our surgical management of the cold nodule includes resection of a lobe and isthmus with exploration and palpation of the opposite side. The nerve is routinely identified and preserved. In the past 18 months, we have experienced two minor postoperative complications in 36 consecutive cases of the lobe and isthmus resection for benign disease.", "contents": "The grand biopsy for the \"cold\" thyroid nodule. A lobe and isthmusectomy is our standard surgical management for the cold nodule of the thyroid. Experience indicates that the great majority of cold nodules prove benign. It is manifest that the attendant morbidity for this surgery be extremely low. Our experience reviewing thyroid surgery for only benign disease indicates that complications are indeed uncommon. Our surgical management of the cold nodule includes resection of a lobe and isthmus with exploration and palpation of the opposite side. The nerve is routinely identified and preserved. In the past 18 months, we have experienced two minor postoperative complications in 36 consecutive cases of the lobe and isthmus resection for benign disease."}
{"id": "PMID:481048", "title": "Bilateral longitudinal temporal bone fractures: a retrospective review of seventeen cases.", "content": "The types of temporal bone fractures, longitudinal and transverse, are reviewed. All cases of bilateral temporal bone fractures at Parkland Memorial Hospital in Dallas over a 10-year period from 1968 to 1978 are reivewed and discussed by the authors. One hundred sixty patients with the diagnosis of base of skull fractures were studied. Fifty-nine of these were temporal bone fractures and 17 of the 59 were bilateral. Of all the base of skull fractures, 10% were bilateral temporal bone fractures and 29% of all temporal bone fractures were bilateral. For each case the method of injury, the extent of damage to hearing and facial nerve function, presence of CSF otorrhea, X-ray findings, and additional complications are summarized and the results discussed. The operative findings of facial nerve decompressions are carefully reviewed. The authors' method of caring for temporal bone fractures is presented.", "contents": "Bilateral longitudinal temporal bone fractures: a retrospective review of seventeen cases. The types of temporal bone fractures, longitudinal and transverse, are reviewed. All cases of bilateral temporal bone fractures at Parkland Memorial Hospital in Dallas over a 10-year period from 1968 to 1978 are reivewed and discussed by the authors. One hundred sixty patients with the diagnosis of base of skull fractures were studied. Fifty-nine of these were temporal bone fractures and 17 of the 59 were bilateral. Of all the base of skull fractures, 10% were bilateral temporal bone fractures and 29% of all temporal bone fractures were bilateral. For each case the method of injury, the extent of damage to hearing and facial nerve function, presence of CSF otorrhea, X-ray findings, and additional complications are summarized and the results discussed. The operative findings of facial nerve decompressions are carefully reviewed. The authors' method of caring for temporal bone fractures is presented."}
{"id": "PMID:481049", "title": "Sinus complications of frontal craniotomy.", "content": "Frontal sinusitis, or inflamatory complications of sinusitis, may occur as a consequence of frontal craniotomy when the sinus has been entered during the craniotomy. This may happen particularly during surgery to treat an aneurysm or to excise a tumor in the sellar area. These complications may also occur if the sinus is entered during routine craniotomy when the sinus is high or asymmetrical, or during reduction of frontal bone fractures. This presentation describes the etiology of such inflammatory complications and describes the approach to treatment of such complications, as well as outlining their prevention.", "contents": "Sinus complications of frontal craniotomy. Frontal sinusitis, or inflamatory complications of sinusitis, may occur as a consequence of frontal craniotomy when the sinus has been entered during the craniotomy. This may happen particularly during surgery to treat an aneurysm or to excise a tumor in the sellar area. These complications may also occur if the sinus is entered during routine craniotomy when the sinus is high or asymmetrical, or during reduction of frontal bone fractures. This presentation describes the etiology of such inflammatory complications and describes the approach to treatment of such complications, as well as outlining their prevention."}
{"id": "PMID:481050", "title": "Acoustic and perceptual comparison of chronic and incipient spastic dysphonia.", "content": "Vocal symptoms of patients with chronic and incipient spastic dysphonia were compared on a number of acoustic and perceptual parameters. Patients with incipient spastic dysphonia displayed less severe strain-strangle phonation, effort, rhythm, and stress. Harshness was the only perceptual parameter on which incipient spastic dysphonics were rated higher than chronic spastic dysphonics. Changes in the acoustic measures of laryngealization, harmonic change, mean vowel duration, and duration ratio between stressed and unstressed vowels accompanied changes in listeners' perception of strain-strangle phonation and effort. The variation in acoustic characteristics such as laryngealization and harmonic change as well as normal phonation indicated that both groups were characterized by a variety of phonatory modes.", "contents": "Acoustic and perceptual comparison of chronic and incipient spastic dysphonia. Vocal symptoms of patients with chronic and incipient spastic dysphonia were compared on a number of acoustic and perceptual parameters. Patients with incipient spastic dysphonia displayed less severe strain-strangle phonation, effort, rhythm, and stress. Harshness was the only perceptual parameter on which incipient spastic dysphonics were rated higher than chronic spastic dysphonics. Changes in the acoustic measures of laryngealization, harmonic change, mean vowel duration, and duration ratio between stressed and unstressed vowels accompanied changes in listeners' perception of strain-strangle phonation and effort. The variation in acoustic characteristics such as laryngealization and harmonic change as well as normal phonation indicated that both groups were characterized by a variety of phonatory modes."}
{"id": "PMID:481051", "title": "Progressive sensorineural hearing loss and cochlear otosclerosis: a prospective study.", "content": "The association of otosclerosis with reduced bone conduction is well known but no experimental or valid clinical relationship has been established to confirm this relationship. The purpose of this presentation is to demonstrate a clinical relationship between otosclerosis and sensorineural hearing loss. Experimental proof will await the accumulation and study of temporal bones of those individuals who, in life, exhibited the clinical relationship to be developed in this dissertation.", "contents": "Progressive sensorineural hearing loss and cochlear otosclerosis: a prospective study. The association of otosclerosis with reduced bone conduction is well known but no experimental or valid clinical relationship has been established to confirm this relationship. The purpose of this presentation is to demonstrate a clinical relationship between otosclerosis and sensorineural hearing loss. Experimental proof will await the accumulation and study of temporal bones of those individuals who, in life, exhibited the clinical relationship to be developed in this dissertation."}
{"id": "PMID:481055", "title": "[Rational combined X-ray examination of gallbladder and stomach (author's transl)].", "content": "X-ray examination of patients with upper abdominal symptoms has been performed up to now mostly as a sequence of separate procedures; instead, an integrated procedure is recommended: a plain film of the upper abdomen should be taken first; a routine oral cholecystogram in the lying position 12 hrs after intake of the contrast medium should be omitted-instead the gallbladder should be visualized fluoroscopically with the patient remaining in the standing position or lying, and films should be taken during visualization. X-ray examination of the stomach and duodenum may follow immediately thereupon, eventually as well during pharmacologically induced hypotony. Then a fatty meal may be given in order to stimulate contraction of the gallbladder; if spasmolytics have been given before, contraction however might be hampered. The diagnostic significance of fatty meals applied as a routine procedure in X-ray diagnosis of gallbladder function is considered to be limited; on the other hand this procedure may improve the diagnosis of the morphological situation.", "contents": "[Rational combined X-ray examination of gallbladder and stomach (author's transl)]. X-ray examination of patients with upper abdominal symptoms has been performed up to now mostly as a sequence of separate procedures; instead, an integrated procedure is recommended: a plain film of the upper abdomen should be taken first; a routine oral cholecystogram in the lying position 12 hrs after intake of the contrast medium should be omitted-instead the gallbladder should be visualized fluoroscopically with the patient remaining in the standing position or lying, and films should be taken during visualization. X-ray examination of the stomach and duodenum may follow immediately thereupon, eventually as well during pharmacologically induced hypotony. Then a fatty meal may be given in order to stimulate contraction of the gallbladder; if spasmolytics have been given before, contraction however might be hampered. The diagnostic significance of fatty meals applied as a routine procedure in X-ray diagnosis of gallbladder function is considered to be limited; on the other hand this procedure may improve the diagnosis of the morphological situation."}
{"id": "PMID:481056", "title": "[Endoscopic polypectomy in the colon, a calculated risk (author's transl)].", "content": "5774 colonoscopies were performed in 5 years, --including 2877 total colonoscopies and 1338 polypectomies. This confirms the diagnostic and therapeutic value of this method. Increasing experience of the endoscopist, better techniques as well as adequate pre-and postoperative care contribute to decreasing the risk of the polypectomy. If coagulation current is used exclusively the risk of bleeding can be lowered considerably.", "contents": "[Endoscopic polypectomy in the colon, a calculated risk (author's transl)]. 5774 colonoscopies were performed in 5 years, --including 2877 total colonoscopies and 1338 polypectomies. This confirms the diagnostic and therapeutic value of this method. Increasing experience of the endoscopist, better techniques as well as adequate pre-and postoperative care contribute to decreasing the risk of the polypectomy. If coagulation current is used exclusively the risk of bleeding can be lowered considerably."}
{"id": "PMID:481057", "title": "[Premedication of esophago-gastro-duodenoscopy with a neuroleptanalgesic drug and a tranquillizer--a controlled clinical study (author's transl)].", "content": "In a controlled double-blind randomized study Thalamonal and Valium were compared in 498 esophago-gastro-duodenoscopies. Both drugs were injected intravenously immediately before the procedure. Satisfactory sedation which made additional injection of the drug during the endoscopy superfluous could be achieved in 66% of the patients with Thalamonal and in 52% with Valium respectively. The difference between the two preparations is statistically significant (p less than 0,005). In 95 patients catamnestic studies were performed in order to evaluate second episodes of fatigue after the endoscopy. A good effect of the preparation during endoscopy and a lack of second attacks of sedation following discharge was observed only in 23% of the patients after Thalamonal and in 21% of the patients after Valium injection, respectively. Therfore a good premedication was achieved in only one fifth of the patients receiving one of the two drugs.", "contents": "[Premedication of esophago-gastro-duodenoscopy with a neuroleptanalgesic drug and a tranquillizer--a controlled clinical study (author's transl)]. In a controlled double-blind randomized study Thalamonal and Valium were compared in 498 esophago-gastro-duodenoscopies. Both drugs were injected intravenously immediately before the procedure. Satisfactory sedation which made additional injection of the drug during the endoscopy superfluous could be achieved in 66% of the patients with Thalamonal and in 52% with Valium respectively. The difference between the two preparations is statistically significant (p less than 0,005). In 95 patients catamnestic studies were performed in order to evaluate second episodes of fatigue after the endoscopy. A good effect of the preparation during endoscopy and a lack of second attacks of sedation following discharge was observed only in 23% of the patients after Thalamonal and in 21% of the patients after Valium injection, respectively. Therfore a good premedication was achieved in only one fifth of the patients receiving one of the two drugs."}
{"id": "PMID:481058", "title": "[Percutaneous cholangiography with the Chiba method (author's transl)].", "content": "In 92 patients with cholestasis a percutaneous cholangiography was performed. The bile duct could be visualized in 75 cases (81.5 percent). When the bile ducts were dilatated, the investigation succeeded in 94 percent, whereas in patients with normal bile ducts the rate was 65 percent. Complications occurred only in 2 cases. In one patient surgery was necessary due to biliary  peritonitis. In another patient a discrete hemobilia without clinical symptoms could be found during laparotomy.", "contents": "[Percutaneous cholangiography with the Chiba method (author's transl)]. In 92 patients with cholestasis a percutaneous cholangiography was performed. The bile duct could be visualized in 75 cases (81.5 percent). When the bile ducts were dilatated, the investigation succeeded in 94 percent, whereas in patients with normal bile ducts the rate was 65 percent. Complications occurred only in 2 cases. In one patient surgery was necessary due to biliary  peritonitis. In another patient a discrete hemobilia without clinical symptoms could be found during laparotomy."}
{"id": "PMID:481059", "title": "[Biliary peritonitis after percutaneous transhepatic cholangiography with the Chiba technique (author's transl)].", "content": "The Chiba technique of percutaneous transhepatic cholangiography (PTC) can be easily learned and does not require much technical equipment; it is thus widely used now in the diagnosis of suspected obstructive jaundice. The procedure is generally regarded as safe; thus standby availability of a surgical outfit is not considered to be necessary. However fever, cholangitis, septicemia, biliary peritonitis and bleeding have been reported in patients who underwent PTC. A case report is given of a patient who had biliary peritonitis following PTC, in order to demonstrate the need for careful selection of patients undergoing this procedure. PTC should not be done in patients with coagulopathy, cholangitis and known allergic reactions against the cntrast medium to be injected. If a dilated duct can be visualized bile should be aspirated and only small amounts of contrast medium be injected. If extrahepatic biliary obstruction has been diagnosed or if the patient complains about pain after the procedure surgery should be done within 24 hours.", "contents": "[Biliary peritonitis after percutaneous transhepatic cholangiography with the Chiba technique (author's transl)]. The Chiba technique of percutaneous transhepatic cholangiography (PTC) can be easily learned and does not require much technical equipment; it is thus widely used now in the diagnosis of suspected obstructive jaundice. The procedure is generally regarded as safe; thus standby availability of a surgical outfit is not considered to be necessary. However fever, cholangitis, septicemia, biliary peritonitis and bleeding have been reported in patients who underwent PTC. A case report is given of a patient who had biliary peritonitis following PTC, in order to demonstrate the need for careful selection of patients undergoing this procedure. PTC should not be done in patients with coagulopathy, cholangitis and known allergic reactions against the cntrast medium to be injected. If a dilated duct can be visualized bile should be aspirated and only small amounts of contrast medium be injected. If extrahepatic biliary obstruction has been diagnosed or if the patient complains about pain after the procedure surgery should be done within 24 hours."}
{"id": "PMID:481060", "title": "[Exploration of pancreaticojejunal anastomosis by postoperative endoscopic pancreaticography (author's transl)].", "content": "Endoscopic pancreaticography (EP) was performed in 9 patients after pancreaticojejunal anastomosis had been established surgically; in 2 of these patients EP was not successful. The anastomosis could be demonstrated in 5 cases to be functioning 1-6 years after operation; in 3 cases the duct system was found to be dilatated, in 2 cases diameters were normal. EP and clinical symptoms correlated well in 4 of 5 cases.", "contents": "[Exploration of pancreaticojejunal anastomosis by postoperative endoscopic pancreaticography (author's transl)]. Endoscopic pancreaticography (EP) was performed in 9 patients after pancreaticojejunal anastomosis had been established surgically; in 2 of these patients EP was not successful. The anastomosis could be demonstrated in 5 cases to be functioning 1-6 years after operation; in 3 cases the duct system was found to be dilatated, in 2 cases diameters were normal. EP and clinical symptoms correlated well in 4 of 5 cases."}
{"id": "PMID:481061", "title": "[Hemobilia after liver biopsy (author's transl)].", "content": "Colicky pain in the gallbladder region, hematemesis, melena and jaundice are symptoms of hemobilia. The underlying cause can be an arterio-biliary or a porto-biliary fistula, resulting in hemorrhage into the duodenum. Shunting may be intra- or extrahepatic and may originate from trauma, or from biliary, pancreatic or vascular lesions. Since 1976 liver biopsies were done in 2221 patients; in 3 of these hemobilia occurred. In one case the artery involved could be obturated by transarterial placement of Ivalon particles; the two other cases recovered without special treatment. A review is given of 23 cases of hemobilia after liver biopsy, and treatment is discussed.", "contents": "[Hemobilia after liver biopsy (author's transl)]. Colicky pain in the gallbladder region, hematemesis, melena and jaundice are symptoms of hemobilia. The underlying cause can be an arterio-biliary or a porto-biliary fistula, resulting in hemorrhage into the duodenum. Shunting may be intra- or extrahepatic and may originate from trauma, or from biliary, pancreatic or vascular lesions. Since 1976 liver biopsies were done in 2221 patients; in 3 of these hemobilia occurred. In one case the artery involved could be obturated by transarterial placement of Ivalon particles; the two other cases recovered without special treatment. A review is given of 23 cases of hemobilia after liver biopsy, and treatment is discussed."}
{"id": "PMID:481062", "title": "[Ultrasound-guided needle biopsy of malignant liver cyst (author's transl)].", "content": "A liver cyst was detected during a routine ultrasonographic examination. Needle biopsy of the cyst was performed using ultrasound as a guidance device during puncture. Malignant cells could be demonstrated on cytological examination. Needle biopsy of circumscribed lesions of the liver is a low risk procedure, causing no strain for the patient. Needle biopsy should be done, when a hepatic cyst is detected by ultrasound, when there is suspicion of malignancy, and when a parasitic lesion has been excluded.", "contents": "[Ultrasound-guided needle biopsy of malignant liver cyst (author's transl)]. A liver cyst was detected during a routine ultrasonographic examination. Needle biopsy of the cyst was performed using ultrasound as a guidance device during puncture. Malignant cells could be demonstrated on cytological examination. Needle biopsy of circumscribed lesions of the liver is a low risk procedure, causing no strain for the patient. Needle biopsy should be done, when a hepatic cyst is detected by ultrasound, when there is suspicion of malignancy, and when a parasitic lesion has been excluded."}
{"id": "PMID:481133", "title": "Phospholipid exchange between subcellular organelles of rabbit lung.", "content": "A soluble protein fraction (PLEP) prepared from rabbit lung can catalyze the exchange of phospholipids between subcellular organelles of the lung and between these subcellular organelles and synthetic liposomes. Phospholipid exchange between microsomes and synthetic liposomes and between mitochondria and synthetic liposomes was stimulated 8-fold and 2.5-fold, respectively, in the presence of the protein fraction. Lung exchange protein could also catalyzed phospholipid exchange between subcellular organelles of the liver and synthetic liposomes. Phospholipid transfer between microsomes and lamellar bodies of the lung was stimulated 2-fold by the exchange protein. Both radiolabeled phosphatidylcholine (PC) and phosphatidylinositol (PI) were transferred from 32P-labeled microsomes to lamellar bodies, but the exchange protein exhibited no transfer activity for phosphatidylglycerol (PG) and that for phosphatidylethanolamine (PE) was insignificant compared to the transfer activity for phosphatidylcholine and phosphatidylinositol. While the physiological role of the phospholipid exchange proteins in the lung is unknown, it is possible that they participate in the distribution of the newly synthesized phospholipids from the site of synthesis to lamellar bodies and other membrane compartments of cells.", "contents": "Phospholipid exchange between subcellular organelles of rabbit lung. A soluble protein fraction (PLEP) prepared from rabbit lung can catalyze the exchange of phospholipids between subcellular organelles of the lung and between these subcellular organelles and synthetic liposomes. Phospholipid exchange between microsomes and synthetic liposomes and between mitochondria and synthetic liposomes was stimulated 8-fold and 2.5-fold, respectively, in the presence of the protein fraction. Lung exchange protein could also catalyzed phospholipid exchange between subcellular organelles of the liver and synthetic liposomes. Phospholipid transfer between microsomes and lamellar bodies of the lung was stimulated 2-fold by the exchange protein. Both radiolabeled phosphatidylcholine (PC) and phosphatidylinositol (PI) were transferred from 32P-labeled microsomes to lamellar bodies, but the exchange protein exhibited no transfer activity for phosphatidylglycerol (PG) and that for phosphatidylethanolamine (PE) was insignificant compared to the transfer activity for phosphatidylcholine and phosphatidylinositol. While the physiological role of the phospholipid exchange proteins in the lung is unknown, it is possible that they participate in the distribution of the newly synthesized phospholipids from the site of synthesis to lamellar bodies and other membrane compartments of cells."}
{"id": "PMID:481134", "title": "The effect of clofibrate on heart and plasma lipids in rats fed a diet containing rapeseed oil.", "content": "The effect of clofibrate on heart and plasma lipids in rats fed a diet containing 30% of the calories as peanut oil (PO) or rapeseed oil (RSO) (42.7% erucic acid and 0.5% eicosenoic acid) was studied. A decrease of erucic acid content to one-third and concomitant increase in the content of 18:1, 16:1 and 16:0 fatty acids in plasma triacylglycerols were observed after administration of clofibrate to rats fed the RSO-diet. It is suggested that these changes reflect the increased capacity of the liver to chain-shorten very long chain length fatty acids. The extent of lipidosis in the heart of rats fed the RSO-diet was decreased by 50% by clofibrate. However, the concentration of erucic acid in heart triacylglycerols decreased much less (30%) than the concentration of all other fatty acids (50-65%). It is concluded that the clofibrate administration increased the oxidative capacity of the heart mitochondria and that the heart cell does not have an efficient system to handle very long chain length monounsaturated fatty acids as does the liver.", "contents": "The effect of clofibrate on heart and plasma lipids in rats fed a diet containing rapeseed oil. The effect of clofibrate on heart and plasma lipids in rats fed a diet containing 30% of the calories as peanut oil (PO) or rapeseed oil (RSO) (42.7% erucic acid and 0.5% eicosenoic acid) was studied. A decrease of erucic acid content to one-third and concomitant increase in the content of 18:1, 16:1 and 16:0 fatty acids in plasma triacylglycerols were observed after administration of clofibrate to rats fed the RSO-diet. It is suggested that these changes reflect the increased capacity of the liver to chain-shorten very long chain length fatty acids. The extent of lipidosis in the heart of rats fed the RSO-diet was decreased by 50% by clofibrate. However, the concentration of erucic acid in heart triacylglycerols decreased much less (30%) than the concentration of all other fatty acids (50-65%). It is concluded that the clofibrate administration increased the oxidative capacity of the heart mitochondria and that the heart cell does not have an efficient system to handle very long chain length monounsaturated fatty acids as does the liver."}
{"id": "PMID:481135", "title": "The metabolic fate of cholesterol-5 alpha, 6 alpha-expoxide in vivo.", "content": "[14C] Cholesterol-5 alpha, 6 alpha-expoxide, administered to mice by either gastric intubation or skin painting, was rapidly and primarily excreted in the feces. Residual amonts of the epoxide and its metabolites were found in a wide variety of organs, and persisted for at least 72 hr. At some sites (principally the liver, the small intestinal contents and the combined stomach/duodenum and their contents), the labeled compound existed in a water-soluble form which could not be extracted with chloroform/methanol. Treatment of the small intestinal contents with a preparation of beta-glucuronidase/sulfatase produced a marked increase in the amount of organic-solvent-extractable cholesterol-alpha-epoxide and other polar metabolites. Unchanged epoxide was found mainly in the feces and the skin at the site of application. On the basis of these results, stool specimens, and not blood samples, should be analyzed to detect the presence of this compound and/or its metabolites in vivo.", "contents": "The metabolic fate of cholesterol-5 alpha, 6 alpha-expoxide in vivo. [14C] Cholesterol-5 alpha, 6 alpha-expoxide, administered to mice by either gastric intubation or skin painting, was rapidly and primarily excreted in the feces. Residual amonts of the epoxide and its metabolites were found in a wide variety of organs, and persisted for at least 72 hr. At some sites (principally the liver, the small intestinal contents and the combined stomach/duodenum and their contents), the labeled compound existed in a water-soluble form which could not be extracted with chloroform/methanol. Treatment of the small intestinal contents with a preparation of beta-glucuronidase/sulfatase produced a marked increase in the amount of organic-solvent-extractable cholesterol-alpha-epoxide and other polar metabolites. Unchanged epoxide was found mainly in the feces and the skin at the site of application. On the basis of these results, stool specimens, and not blood samples, should be analyzed to detect the presence of this compound and/or its metabolites in vivo."}
{"id": "PMID:481136", "title": "Epoxides as products of lipid autoxidation in rat lungs.", "content": "The nature and content of lipid epoxides in rat lung were examined in air-breathing control rats and those exposed to nitrogen dioxide. Exposure to 6.5 ppm NO2 for 24 hr resulted in significantly greater epoxide content in a number of lipid classes. It is proposed that lipid autoxidation in lung tissues may contribute to the levels of epoxide-containing lipids. Furthermore, the processes involved in epoxide formation may be predicted from autoxidation studies utilizing a system of unsaturated fatty acid monolayers on silica gel which serves as a model for biomembranes. The findings indicate that exposure to oxidizing gases can lead to an accumulation of lipid epoxides in both lung parenchymal tissue and on the alveolar surface.", "contents": "Epoxides as products of lipid autoxidation in rat lungs. The nature and content of lipid epoxides in rat lung were examined in air-breathing control rats and those exposed to nitrogen dioxide. Exposure to 6.5 ppm NO2 for 24 hr resulted in significantly greater epoxide content in a number of lipid classes. It is proposed that lipid autoxidation in lung tissues may contribute to the levels of epoxide-containing lipids. Furthermore, the processes involved in epoxide formation may be predicted from autoxidation studies utilizing a system of unsaturated fatty acid monolayers on silica gel which serves as a model for biomembranes. The findings indicate that exposure to oxidizing gases can lead to an accumulation of lipid epoxides in both lung parenchymal tissue and on the alveolar surface."}
{"id": "PMID:481137", "title": "Studies on cell proliferation in inguinal adipose tissue during early development in the rat.", "content": "[2-14C] Thymidine was injected into rats aged 3,5 and 10 days, and incorporation of the precursor into deoxyribonucleic acid (DNA) of the inguinal fat tissue was measured for short time periods. Using chromatographic procedures to measure the distribution of thymidine and its metabolites in the soluble fraction of the tissue, degradation of the precursor was found to be similar at all ages. The data indicate that thymidine was more rapidly utilized for DNA synthesis in 3-day-old rats than in older animals. When 14C-thymidine was injected in vivo and adipocytes and stromal cells were then separated from the inguinal tissue of 3-and 5-day-old rats, the incorporation into DNA was significant in both types of cells already 30 min after pulse labeling. Stromal cells took up twice as much of label as the adipocytes. Furthermore, real incorporation into DNA was found in the adipocytes when incubated in vitro in a culture medium supplemented with 14C-thymidine. The possibility is discussed that early in postnatal life adipocytes might synthesize DNA for further cell division.", "contents": "Studies on cell proliferation in inguinal adipose tissue during early development in the rat. [2-14C] Thymidine was injected into rats aged 3,5 and 10 days, and incorporation of the precursor into deoxyribonucleic acid (DNA) of the inguinal fat tissue was measured for short time periods. Using chromatographic procedures to measure the distribution of thymidine and its metabolites in the soluble fraction of the tissue, degradation of the precursor was found to be similar at all ages. The data indicate that thymidine was more rapidly utilized for DNA synthesis in 3-day-old rats than in older animals. When 14C-thymidine was injected in vivo and adipocytes and stromal cells were then separated from the inguinal tissue of 3-and 5-day-old rats, the incorporation into DNA was significant in both types of cells already 30 min after pulse labeling. Stromal cells took up twice as much of label as the adipocytes. Furthermore, real incorporation into DNA was found in the adipocytes when incubated in vitro in a culture medium supplemented with 14C-thymidine. The possibility is discussed that early in postnatal life adipocytes might synthesize DNA for further cell division."}
{"id": "PMID:481138", "title": "Stimulation of lipid absorption in young rats by cholesterol: early time changes and effects on pentobarbital sleeping time.", "content": "Four groups of young male and female rats were fed a chow diet (O), chow plus 10% corn oil (F), chow plus 1% cholesterol (C), or chow plus 1% cholesterol plus 10% corn oil (CF) for 1, 2, 4 and 8 days. After 2 dats, male F, C and CF rats exhibited a shorter anesthesia period (-20 to -30%) when given pentobarbital. By 4 days, male F and C rats had pentobarbital sleeping times (PB-ST) 20% less than O rats. These effects were additive and CF rats had 40% shorter PB-ST. Reduction of PB-ST by cholesterol and corn oil was similar but slightly less in female rats. Liver lipid content doubled in 4 days in CF rats, and liver cholesterol was 4 times than of O rats. These changes and the increases in metabolism of barbiturate suggested changes in liver microsomal enzyme activities. Serum glutamic oxaloacetic and glutamic pyruvic transaminase, two enzymes reflective of liver damage, did not increase after 8 days on C, F or CF diets. Our results suggest that consumption of an animal sterol and a high lipid diet by laboratory rats, normally consuming a diet low in fat (3-4%), increase the ability of the animal to detoxify a barbiturate. Storage of absorbed dietary cholesterol in the liver may represent a major mechanism for maintaining extra hepatic cholesterol homeostasis.", "contents": "Stimulation of lipid absorption in young rats by cholesterol: early time changes and effects on pentobarbital sleeping time. Four groups of young male and female rats were fed a chow diet (O), chow plus 10% corn oil (F), chow plus 1% cholesterol (C), or chow plus 1% cholesterol plus 10% corn oil (CF) for 1, 2, 4 and 8 days. After 2 dats, male F, C and CF rats exhibited a shorter anesthesia period (-20 to -30%) when given pentobarbital. By 4 days, male F and C rats had pentobarbital sleeping times (PB-ST) 20% less than O rats. These effects were additive and CF rats had 40% shorter PB-ST. Reduction of PB-ST by cholesterol and corn oil was similar but slightly less in female rats. Liver lipid content doubled in 4 days in CF rats, and liver cholesterol was 4 times than of O rats. These changes and the increases in metabolism of barbiturate suggested changes in liver microsomal enzyme activities. Serum glutamic oxaloacetic and glutamic pyruvic transaminase, two enzymes reflective of liver damage, did not increase after 8 days on C, F or CF diets. Our results suggest that consumption of an animal sterol and a high lipid diet by laboratory rats, normally consuming a diet low in fat (3-4%), increase the ability of the animal to detoxify a barbiturate. Storage of absorbed dietary cholesterol in the liver may represent a major mechanism for maintaining extra hepatic cholesterol homeostasis."}
{"id": "PMID:481139", "title": "Preparation of cupric palmitate membrane, its characterization and evaluation of thermodynamically effective fixed charge density.", "content": "Membrane potentials have been measured across parchment-supported cupric palmitate membrane separating various 1:1 electrolytes at concentrations C1 and C2 such that C2 = 10 C1. Membrane potential data have been used to calculate transference number of ions, permselectivity and also to derive the thermodynamically effective fixed charge density which is an important characteristic governing the membrane phenomena by utilizing the generally accepted and most widely used theory of Teorell-Meyer and Sievers as well as the recent theories for membrane potential of Kobatake et al. and Nagasawa et al. based on the principles of nonequilibrium thermodynamics. The values of charge densities derived from different theories were almost the same, confirming thereby the validity of the recently developed theories of membrane potential.", "contents": "Preparation of cupric palmitate membrane, its characterization and evaluation of thermodynamically effective fixed charge density. Membrane potentials have been measured across parchment-supported cupric palmitate membrane separating various 1:1 electrolytes at concentrations C1 and C2 such that C2 = 10 C1. Membrane potential data have been used to calculate transference number of ions, permselectivity and also to derive the thermodynamically effective fixed charge density which is an important characteristic governing the membrane phenomena by utilizing the generally accepted and most widely used theory of Teorell-Meyer and Sievers as well as the recent theories for membrane potential of Kobatake et al. and Nagasawa et al. based on the principles of nonequilibrium thermodynamics. The values of charge densities derived from different theories were almost the same, confirming thereby the validity of the recently developed theories of membrane potential."}
{"id": "PMID:481150", "title": "Cardiac output differences in males and females during mild cycle ergometer exercise.", "content": "To study sex differences in cardiac output during submaximum exercise, eight male and eight female subjects were matched on max Vo2 (1 min-1 and ml kg-1 min-1). Each subject performed one, eight minute submaximum bicycle ergometer test at 35% of Vo2max (300 kpm min-1). At a steady state mean Vo2 of 0.96 1 min-1, cardiac output was determined. Significant differences between men and women were observed (p less than .05) in cardiac output, stroke volume and (a-v) O2 difference. The males had a lower cardiac output (1.75 1 min-1) and stroke volume (17.2 ml beat-1) and a higher (a-v) O2 difference (2.01 vol %). When these variables were expressed independent of lean body weight the above differences were non-significant. It was suggested that cardiac output differences between the sexes during mild exercise are due, in part, to differences in lean body weight.", "contents": "Cardiac output differences in males and females during mild cycle ergometer exercise. To study sex differences in cardiac output during submaximum exercise, eight male and eight female subjects were matched on max Vo2 (1 min-1 and ml kg-1 min-1). Each subject performed one, eight minute submaximum bicycle ergometer test at 35% of Vo2max (300 kpm min-1). At a steady state mean Vo2 of 0.96 1 min-1, cardiac output was determined. Significant differences between men and women were observed (p less than .05) in cardiac output, stroke volume and (a-v) O2 difference. The males had a lower cardiac output (1.75 1 min-1) and stroke volume (17.2 ml beat-1) and a higher (a-v) O2 difference (2.01 vol %). When these variables were expressed independent of lean body weight the above differences were non-significant. It was suggested that cardiac output differences between the sexes during mild exercise are due, in part, to differences in lean body weight."}
{"id": "PMID:481151", "title": "Characteristics of national and world class female pentathletes.", "content": "Nine national and world-class female pentathletes were assessed for body composition, maximal aerobic power (Vo2max), strength, and speed. The subjects averaged 175.4 cm in height, 65.4 kg in weight, 11.0% relative body fat, 45.9 ml kg min-1 in Vo2max, and 8.35 m/sec for maximal running speed. When compared with other athletic females, this group was tall and lean with good, but not exceptional, maximal aerobic power. Group means for one-repetition maximum lifts were 59.7 kg (power clean), 61.5 kg (horizontal bench press), 60.0 kg (pull down), 87.9 kg (squat), 44.9 kg (include bench press), and 36.3 kg (leg curl). Within this elite group the most successful performers were stronger and possessed a greater lean body weight which together suggest the importance of muscle mass for success in the pentathlon.", "contents": "Characteristics of national and world class female pentathletes. Nine national and world-class female pentathletes were assessed for body composition, maximal aerobic power (Vo2max), strength, and speed. The subjects averaged 175.4 cm in height, 65.4 kg in weight, 11.0% relative body fat, 45.9 ml kg min-1 in Vo2max, and 8.35 m/sec for maximal running speed. When compared with other athletic females, this group was tall and lean with good, but not exceptional, maximal aerobic power. Group means for one-repetition maximum lifts were 59.7 kg (power clean), 61.5 kg (horizontal bench press), 60.0 kg (pull down), 87.9 kg (squat), 44.9 kg (include bench press), and 36.3 kg (leg curl). Within this elite group the most successful performers were stronger and possessed a greater lean body weight which together suggest the importance of muscle mass for success in the pentathlon."}
{"id": "PMID:481152", "title": "Prediction of maximal oxygen uptake in boys, ages 7-15 years.", "content": "Predictions of Vo2max (1 min-1 and ml kg-1 min-1) were obtained via multiple regression procedures from a sample of 100 boys, ages 6.7-14.8 years. Prediction equations for Vo2max (1 min-1) were obtained from the subjects' height, and the Vo2 (1 min-1) and heart rate observed during the third min of a treadmill walk (R = 0.95; CV = +/- 9.3%). A similar prediction was obtained when the subjects' age, height and weight were used (R = -0.94; CV = +/- 9.7%). Vo2max (ml kg-1 min-1) was predicted with similar accuracy (CV = +/- 8.4%) from age, and heart rate, VCO2(1 min-1), Vo2 (ml kg min-1), or from simply age, height and weight (CV = +/- 9.2%). Cross-validation of the equations with another sample of 39 boys demonstrated that the prediction equations based on laboratory data were quite stable, % errors approximately 1-2 +/- 9%. However, the equations based on age, height and weight underestimated the Vo2max slightly, both in 1 min (X = -0.091 min) and ml kg min (X = -2.2 ml kg min, P less than 0.05). The results indicate that reasonably reliable and accurate estimates of Vo2max for children may be obtained from either laboratory data, or simply from their age, height and weight.", "contents": "Prediction of maximal oxygen uptake in boys, ages 7-15 years. Predictions of Vo2max (1 min-1 and ml kg-1 min-1) were obtained via multiple regression procedures from a sample of 100 boys, ages 6.7-14.8 years. Prediction equations for Vo2max (1 min-1) were obtained from the subjects' height, and the Vo2 (1 min-1) and heart rate observed during the third min of a treadmill walk (R = 0.95; CV = +/- 9.3%). A similar prediction was obtained when the subjects' age, height and weight were used (R = -0.94; CV = +/- 9.7%). Vo2max (ml kg-1 min-1) was predicted with similar accuracy (CV = +/- 8.4%) from age, and heart rate, VCO2(1 min-1), Vo2 (ml kg min-1), or from simply age, height and weight (CV = +/- 9.2%). Cross-validation of the equations with another sample of 39 boys demonstrated that the prediction equations based on laboratory data were quite stable, % errors approximately 1-2 +/- 9%. However, the equations based on age, height and weight underestimated the Vo2max slightly, both in 1 min (X = -0.091 min) and ml kg min (X = -2.2 ml kg min, P less than 0.05). The results indicate that reasonably reliable and accurate estimates of Vo2max for children may be obtained from either laboratory data, or simply from their age, height and weight."}
{"id": "PMID:481153", "title": "Control of sweating rate while exercising in the heat.", "content": "All of the heat produced during exercise in a hot environment is either stored in the body or dissipated to the environment by evaporation of sweat. In order to minimize the storage of heat, thereby resisting circulatory strain, the body must be able to effectively increase sweating rate in these conditions. This paper characterizes the physiological control of sweating as a proportional control system, with increases in internal temperature having the greatest effect upon increasing the sweating rate. Factors which modify the threshold and gain of the sweating response, such as occur with dehydration or physical training, affect the storage of heat and therefore the steady state internal temperature during exercise in the heat.", "contents": "Control of sweating rate while exercising in the heat. All of the heat produced during exercise in a hot environment is either stored in the body or dissipated to the environment by evaporation of sweat. In order to minimize the storage of heat, thereby resisting circulatory strain, the body must be able to effectively increase sweating rate in these conditions. This paper characterizes the physiological control of sweating as a proportional control system, with increases in internal temperature having the greatest effect upon increasing the sweating rate. Factors which modify the threshold and gain of the sweating response, such as occur with dehydration or physical training, affect the storage of heat and therefore the steady state internal temperature during exercise in the heat."}
{"id": "PMID:481154", "title": "Control of skin circulation during exercise and heat stress.", "content": "At any given environmental and mean skin temperature, exercise brings about an increase in internal body temperature and skin blood flow. At high environmental temperatures, when skin temperature is elevated, skin blood flow at any given internal temperature reaches higher levels than at cooler skin temperatures. Increased cutaneous blood flow serves to deliver metabolic heat from the core to the skin, where the heat is lost to the environment by convective, radiative, and evaporative mechanisms. However, at high levels of skin blood flow, peripheral vascular pooling and fluid losses by filtration lead to reduced central venous pressure. This lowers cardiac stroke volume, and requires a higher heart rate to maintain a given cardiac output. Mechanisms which alleviate some of the cardiovascular strain produced by exercise in the heat include the following: acutely, reflexes which arise from receptors in working muscles produce vasoconstriction in a number of central and peripheral vascular beds. Other reflexes, arising from cardiac baroreceptors, produce additional peripheral vasoconstriction when cardiac filling is impaired. In the long term, physical conditioning and heat acclimation lead to increases in sweat output during thermal stress, leading to cooler skin and core temperature during exercise, and decreasing the level of skin blood flow needed for regulation of body temperature.", "contents": "Control of skin circulation during exercise and heat stress. At any given environmental and mean skin temperature, exercise brings about an increase in internal body temperature and skin blood flow. At high environmental temperatures, when skin temperature is elevated, skin blood flow at any given internal temperature reaches higher levels than at cooler skin temperatures. Increased cutaneous blood flow serves to deliver metabolic heat from the core to the skin, where the heat is lost to the environment by convective, radiative, and evaporative mechanisms. However, at high levels of skin blood flow, peripheral vascular pooling and fluid losses by filtration lead to reduced central venous pressure. This lowers cardiac stroke volume, and requires a higher heart rate to maintain a given cardiac output. Mechanisms which alleviate some of the cardiovascular strain produced by exercise in the heat include the following: acutely, reflexes which arise from receptors in working muscles produce vasoconstriction in a number of central and peripheral vascular beds. Other reflexes, arising from cardiac baroreceptors, produce additional peripheral vasoconstriction when cardiac filling is impaired. In the long term, physical conditioning and heat acclimation lead to increases in sweat output during thermal stress, leading to cooler skin and core temperature during exercise, and decreasing the level of skin blood flow needed for regulation of body temperature."}
{"id": "PMID:481155", "title": "Effects of exercise in the heat on body fluid distribution.", "content": "Experimental findings as to body fluid shifts during exercise appear to be greatly influenced by the mode of exercise (bicycle ergometer, treadmill, etc) and by the state of subject hydration. Endurance training has been shown to increase resting plasma (blood) volume. Also, endurance training results in modification of vascular volume dynamics during exercise, i.e. for a set task, plasma volume becomes stabilized. In the untrained individual, heat exposure exaggerates body fluid shifts during exercise. With training, stability of vascular volume is attained during heat exposure, but maximum protective responses towards exercise in heat is only gained upon heat acclimatization. Two items benefit the individual: an increase in the capacity of the sweat mechanism and an expansion of plasma volume. Benefits of training as to body fluid shifts are probably a result of metabolic changes within the active muscle mass.", "contents": "Effects of exercise in the heat on body fluid distribution. Experimental findings as to body fluid shifts during exercise appear to be greatly influenced by the mode of exercise (bicycle ergometer, treadmill, etc) and by the state of subject hydration. Endurance training has been shown to increase resting plasma (blood) volume. Also, endurance training results in modification of vascular volume dynamics during exercise, i.e. for a set task, plasma volume becomes stabilized. In the untrained individual, heat exposure exaggerates body fluid shifts during exercise. With training, stability of vascular volume is attained during heat exposure, but maximum protective responses towards exercise in heat is only gained upon heat acclimatization. Two items benefit the individual: an increase in the capacity of the sweat mechanism and an expansion of plasma volume. Benefits of training as to body fluid shifts are probably a result of metabolic changes within the active muscle mass."}
{"id": "PMID:481156", "title": "Heat tolerance and aging.", "content": "Although children and older adults appear more susceptible to heat stress, the mechanisms responsible for their lower tolerance are not fully understood. Many studies dealing with the effect of age on temperature regulation have concluded that an inadequate sweating response is primarily responsible for the low tolerance of children and the elderly to exercise in the heat. However, the dependence of core temperature on relative exercise intensity and sweat rate on absolute exercise intensity makes it difficult to avoid the confounding effects of exercise on thermoregulation when aerobic power (Vo2max) varies across age groups. When 38 non-acclimatized females, ages 12 to 68 years, exercised at 30-35% Vo2max in the heat, the degree of cardiovascular stability was the primary predictor of tolerance time. Age was not a significant predictor. However, it was evident that individuals at either end of the age continuum were more likely to be at risk. For children this risk was associated with the instability of an immature cardiovascular system; for older women, a marked decrement in aerobic power. Sweat rate added significantly to the prediction of tolerance time for all subjects regardless of age. Whether the decrease in responsiveness of sweating noted for some older individuals is an age related change or a reflection of their lower fitness levels is not known.", "contents": "Heat tolerance and aging. Although children and older adults appear more susceptible to heat stress, the mechanisms responsible for their lower tolerance are not fully understood. Many studies dealing with the effect of age on temperature regulation have concluded that an inadequate sweating response is primarily responsible for the low tolerance of children and the elderly to exercise in the heat. However, the dependence of core temperature on relative exercise intensity and sweat rate on absolute exercise intensity makes it difficult to avoid the confounding effects of exercise on thermoregulation when aerobic power (Vo2max) varies across age groups. When 38 non-acclimatized females, ages 12 to 68 years, exercised at 30-35% Vo2max in the heat, the degree of cardiovascular stability was the primary predictor of tolerance time. Age was not a significant predictor. However, it was evident that individuals at either end of the age continuum were more likely to be at risk. For children this risk was associated with the instability of an immature cardiovascular system; for older women, a marked decrement in aerobic power. Sweat rate added significantly to the prediction of tolerance time for all subjects regardless of age. Whether the decrease in responsiveness of sweating noted for some older individuals is an age related change or a reflection of their lower fitness levels is not known."}
{"id": "PMID:481157", "title": "Relationships among training, heat acclimation, and heat tolerance in men and women: the controversy revisited.", "content": "For more than a decade there has been a controversy over the beneficial effects of physical training in a cool environment on exercise-heat tolerance. The issues include (a) inadequate controls regarding the physical characteristics of subjects, (b) marked variability in the conditions of the standard heat tolerance tests and (c) differences in the intensity and duration of the training programs employed. Intense training in a cool environment cannot serve as a substitute for exercise in the heat if acclimation is desired within a 2 week period. However, a substantial improvement (50%) in heat tolerance can be derived from 8-11 weeks of training under temperate conditions (21 degrees C) and thermal equilibrium can be maintained for at least 4 hours during mild work (200 W/m2) in dry or wet heat by endurance runners. These adjustments occur in both men and women and appear to be independent of aerobic capacity. The key to improved thermal tolerance with training in a cool environment is maintenance of an elevated core temperature for a sufficient duration of time to produce an adaptive response.", "contents": "Relationships among training, heat acclimation, and heat tolerance in men and women: the controversy revisited. For more than a decade there has been a controversy over the beneficial effects of physical training in a cool environment on exercise-heat tolerance. The issues include (a) inadequate controls regarding the physical characteristics of subjects, (b) marked variability in the conditions of the standard heat tolerance tests and (c) differences in the intensity and duration of the training programs employed. Intense training in a cool environment cannot serve as a substitute for exercise in the heat if acclimation is desired within a 2 week period. However, a substantial improvement (50%) in heat tolerance can be derived from 8-11 weeks of training under temperate conditions (21 degrees C) and thermal equilibrium can be maintained for at least 4 hours during mild work (200 W/m2) in dry or wet heat by endurance runners. These adjustments occur in both men and women and appear to be independent of aerobic capacity. The key to improved thermal tolerance with training in a cool environment is maintenance of an elevated core temperature for a sufficient duration of time to produce an adaptive response."}
{"id": "PMID:481158", "title": "Influence of caffeine and carbohydrate feedings on endurance performance.", "content": "Nine trained cyclists were studied to determine the effects of caffeine (CAF), and glucose polymer (GP) feedings on work production (kpm) during two hr of isokinetic cycling exercise (80 rpm). Ingestion of 250 mg of CAF 60 min prior to the ride was followed by ingestion of an additional 250 mg fed at 15 min intervals over the first 90 min of the exercise. This treatment significantly increased work production by 7.4% and Vo2 by 7.3% as compared to control (C) while the subjects' perception of exertion remained unchanged. Ingestion of approximately 90 g of GP during the first 90 min (12.8 g/15 min) of the exercise had no effect on total work production or Vo2. It was, however, effective in reducing the rate of fatigue over the last 30 min of cycling. Although GP maintained blood glucose and insulin levels (P less than or equal to 0.05) above those of the C and CAF trials, total CHO utilization did not differ between treatments. During the last 70 min of the CAF trial, however, fat oxidation was elevated 31% and appeared to provide the substrate needed for the increased work production during this period of exercise. These data, therefore, demonstrate an enhanced rate of lipid catabolism and work production following the ingestion of caffeine.", "contents": "Influence of caffeine and carbohydrate feedings on endurance performance. Nine trained cyclists were studied to determine the effects of caffeine (CAF), and glucose polymer (GP) feedings on work production (kpm) during two hr of isokinetic cycling exercise (80 rpm). Ingestion of 250 mg of CAF 60 min prior to the ride was followed by ingestion of an additional 250 mg fed at 15 min intervals over the first 90 min of the exercise. This treatment significantly increased work production by 7.4% and Vo2 by 7.3% as compared to control (C) while the subjects' perception of exertion remained unchanged. Ingestion of approximately 90 g of GP during the first 90 min (12.8 g/15 min) of the exercise had no effect on total work production or Vo2. It was, however, effective in reducing the rate of fatigue over the last 30 min of cycling. Although GP maintained blood glucose and insulin levels (P less than or equal to 0.05) above those of the C and CAF trials, total CHO utilization did not differ between treatments. During the last 70 min of the CAF trial, however, fat oxidation was elevated 31% and appeared to provide the substrate needed for the increased work production during this period of exercise. These data, therefore, demonstrate an enhanced rate of lipid catabolism and work production following the ingestion of caffeine."}
{"id": "PMID:481159", "title": "[Determination of the law of radiation source motion in intracavitary moving and multipositional irradiation].", "content": "The intracavital moving and multipositional irradiation with portable sources in gamma-therapy of extended pathologic foci offers more possibilities for the formation of dose distribution in accordance with the patient's individual features. A method is proposed to calculate the movement law for the point and linear irradiation sources following the preset dose distribution. The authors show possible formation of dose fields with a practically rectilinear isodose portion.", "contents": "[Determination of the law of radiation source motion in intracavitary moving and multipositional irradiation]. The intracavital moving and multipositional irradiation with portable sources in gamma-therapy of extended pathologic foci offers more possibilities for the formation of dose distribution in accordance with the patient's individual features. A method is proposed to calculate the movement law for the point and linear irradiation sources following the preset dose distribution. The authors show possible formation of dose fields with a practically rectilinear isodose portion."}
{"id": "PMID:481160", "title": "[Analog model of the interaction of the human body-temperature sensor-environment system].", "content": "A simple analog model of interaction in the system of human body and temperature sensor is described. It can be employed as an imitator in the development and adjustment of thermometric devices. Analytical expressions which describe this model operation and results of determining the real parameters of the system are reported.", "contents": "[Analog model of the interaction of the human body-temperature sensor-environment system]. A simple analog model of interaction in the system of human body and temperature sensor is described. It can be employed as an imitator in the development and adjustment of thermometric devices. Analytical expressions which describe this model operation and results of determining the real parameters of the system are reported."}
{"id": "PMID:481162", "title": "[Effect of high-amplitude electrical impulses on the strength of the coagulative adhesion of the blood vessels].", "content": "Strength of coagulative commisure of the blood vessel tissues in their coagulation with high-frequency current can be increased after additional action of high-amplitude electrical pulses. A comparative test of commisures tensile strength confirmed its possible increase by 1.6--1.7 times when vascular tissues were preliminarily subjected to the action of short high-amplitude electrical pulses.", "contents": "[Effect of high-amplitude electrical impulses on the strength of the coagulative adhesion of the blood vessels]. Strength of coagulative commisure of the blood vessel tissues in their coagulation with high-frequency current can be increased after additional action of high-amplitude electrical pulses. A comparative test of commisures tensile strength confirmed its possible increase by 1.6--1.7 times when vascular tissues were preliminarily subjected to the action of short high-amplitude electrical pulses."}
{"id": "PMID:481164", "title": "[Gipospast-1 thermoelectric apparatus for local hypothermia].", "content": "A possibility of providing local hypothermia for human extremities over the area from 6.25 cm2 to 37.5 cm2 with temperature level up to 10 degrees C is shown. \"Gipospast-1\" is designed for accomplishing the local hypothermia. It was revealed that at such conditions the human organism reaction ensures a specific heat flow on the cooling surface to 0.02+/-0.04 W/cm2. The clinical trials of the apparatus confirmed its efficacy for removing the contractures of the facial muscles and for decreasing the spastics in paretic extremities of neurosurgical patients.", "contents": "[Gipospast-1 thermoelectric apparatus for local hypothermia]. A possibility of providing local hypothermia for human extremities over the area from 6.25 cm2 to 37.5 cm2 with temperature level up to 10 degrees C is shown. \"Gipospast-1\" is designed for accomplishing the local hypothermia. It was revealed that at such conditions the human organism reaction ensures a specific heat flow on the cooling surface to 0.02+/-0.04 W/cm2. The clinical trials of the apparatus confirmed its efficacy for removing the contractures of the facial muscles and for decreasing the spastics in paretic extremities of neurosurgical patients."}
{"id": "PMID:481165", "title": "[Manufacture of control knobs for medical devices and apparatus].", "content": "Control knobs having rigid projections in the setting port which incise into the shaft of the control element possess operating advantages over the knobs of other constructions. But they are unprofitable in production because of the mechanical treatment method. Considerably technical and economical higher profit can be attained by casting the knobs according to melted models.", "contents": "[Manufacture of control knobs for medical devices and apparatus]. Control knobs having rigid projections in the setting port which incise into the shaft of the control element possess operating advantages over the knobs of other constructions. But they are unprofitable in production because of the mechanical treatment method. Considerably technical and economical higher profit can be attained by casting the knobs according to melted models."}
{"id": "PMID:481167", "title": "[Electrode for the pickup of bioelectrical potentials].", "content": "Three versions of surface multipoint electrodes are described providing electrical contact with the body surface without any electrode paste. The electrical contact is achieved through numerous metal pins being electrically connected with each other. One electrode has all its pins fixed rigidly on a common base-plate. In two other versions the pins are movable by means of special springs and volumetric elastic (springy) materials which allows to ensure electrical contact with uneven body surface.", "contents": "[Electrode for the pickup of bioelectrical potentials]. Three versions of surface multipoint electrodes are described providing electrical contact with the body surface without any electrode paste. The electrical contact is achieved through numerous metal pins being electrically connected with each other. One electrode has all its pins fixed rigidly on a common base-plate. In two other versions the pins are movable by means of special springs and volumetric elastic (springy) materials which allows to ensure electrical contact with uneven body surface."}
{"id": "PMID:481168", "title": "[Design and production problems with stethophonoendoscopes].", "content": "The existing stethophonendoscope models with separate stethoscopic and phonendoscopic heads fail to give adequate reproduction of processes under investigation within the auscultation frequency range with intensity sufficient for reliable diagnosis. The Leningrad industrial association \"Krasnogvardeets\" offers a new stethophonendoscope design with a head reproducing acoustic signs at a necessary intensity level within a wide frequency range. These stethophonendoscopes can be manufactured serially because their most complicated and functionally responsible elements are strictly unified for different models.", "contents": "[Design and production problems with stethophonoendoscopes]. The existing stethophonendoscope models with separate stethoscopic and phonendoscopic heads fail to give adequate reproduction of processes under investigation within the auscultation frequency range with intensity sufficient for reliable diagnosis. The Leningrad industrial association \"Krasnogvardeets\" offers a new stethophonendoscope design with a head reproducing acoustic signs at a necessary intensity level within a wide frequency range. These stethophonendoscopes can be manufactured serially because their most complicated and functionally responsible elements are strictly unified for different models."}
{"id": "PMID:481166", "title": "[Reinforcement of the operating elements of medical instruments].", "content": "Reinforcement processes were analysed to assess the possibility of their use for the medical apparatus production. It was shown that one of the ways to make medical instruments more durable is the reinforcement of their operating surfaces with wear-proof, corrosion-resistant and hard materials by means of soldering, sputtering and fuse on.", "contents": "[Reinforcement of the operating elements of medical instruments]. Reinforcement processes were analysed to assess the possibility of their use for the medical apparatus production. It was shown that one of the ways to make medical instruments more durable is the reinforcement of their operating surfaces with wear-proof, corrosion-resistant and hard materials by means of soldering, sputtering and fuse on."}
{"id": "PMID:481177", "title": "[Evaluation of the force loads on the elements of artificial heart valves from the results of processing echocardiograms].", "content": "Approaches to further improving the valve prosthesis construction greatly depend on the knowledge of the physical load affecting the elements of artificial cardiac valves that operate in the human organism. An analysis of echocardiograms is given. They were recorded in the patients with implanted artificial ball heart valves, type MK4-2.25. The design values for the force of the ball impact against the valve shell are presented. The echocardiogram analysis revealed a phenomenon of the fibrotic ring deformation under the ball action on the valve shell and the degree of this deformation has been determined.", "contents": "[Evaluation of the force loads on the elements of artificial heart valves from the results of processing echocardiograms]. Approaches to further improving the valve prosthesis construction greatly depend on the knowledge of the physical load affecting the elements of artificial cardiac valves that operate in the human organism. An analysis of echocardiograms is given. They were recorded in the patients with implanted artificial ball heart valves, type MK4-2.25. The design values for the force of the ball impact against the valve shell are presented. The echocardiogram analysis revealed a phenomenon of the fibrotic ring deformation under the ball action on the valve shell and the degree of this deformation has been determined."}
{"id": "PMID:481178", "title": "[Value of liver needle-biopsy in Djibouti (1.1110 specimens) (author's transl)].", "content": "Liver diseases, specially hepatomegalies and hepatosplenomegalies are very frequent in Republic of Dijibouti. Needle-biopsy of the liver is a good approach of this pathology as demonstrated by controlling 1.110 specimens within a period of 9 years, leading to very different diagnosis.", "contents": "[Value of liver needle-biopsy in Djibouti (1.1110 specimens) (author's transl)]. Liver diseases, specially hepatomegalies and hepatosplenomegalies are very frequent in Republic of Dijibouti. Needle-biopsy of the liver is a good approach of this pathology as demonstrated by controlling 1.110 specimens within a period of 9 years, leading to very different diagnosis."}
{"id": "PMID:481179", "title": "[Distribution of HBs antigen in Tananarive (author's transl)].", "content": "This survey has been conducted among blood donors and patients with hepatitis, cirrhosis, liver carcinomas and various causes of liver dysfunction or enlargement. The frequency is 5,4 p. 100 among blood donors and 50 p. 100 among cases of hepatitis.", "contents": "[Distribution of HBs antigen in Tananarive (author's transl)]. This survey has been conducted among blood donors and patients with hepatitis, cirrhosis, liver carcinomas and various causes of liver dysfunction or enlargement. The frequency is 5,4 p. 100 among blood donors and 50 p. 100 among cases of hepatitis."}
{"id": "PMID:481180", "title": "[Chatelguyon thermal springs and amoebiasis (author's transl)].", "content": "Ch\u00e2telguyon thermal springs are an effective treatment of chronic post amoebic colitis and its side effects on gastroduodenal tract and autonomic nervous system. The high level of magnesium of these waters is the main therapeutic agent; after the cure, the intraerythrocytic magnesium level is above the standard while the Mg seric level is below. The waters might reactive the amoebiases, increasing in the same time the specific amoebic antibodies. Parasiticidal treatment must be prescribed before any cure, if patient is proved to be Entamoeba minuta or cyst carrier.", "contents": "[Chatelguyon thermal springs and amoebiasis (author's transl)]. Ch\u00e2telguyon thermal springs are an effective treatment of chronic post amoebic colitis and its side effects on gastroduodenal tract and autonomic nervous system. The high level of magnesium of these waters is the main therapeutic agent; after the cure, the intraerythrocytic magnesium level is above the standard while the Mg seric level is below. The waters might reactive the amoebiases, increasing in the same time the specific amoebic antibodies. Parasiticidal treatment must be prescribed before any cure, if patient is proved to be Entamoeba minuta or cyst carrier."}
{"id": "PMID:481181", "title": "[Rheumatoid polyarthritis in Madagascar (author's transl)].", "content": "Report of 11 cases of rheumatoid arthritis observed in Malagasy. Frequently, a single joint is concerned at the on-set period and this may raise some questions to differetiate rheumatic fever, gout and osteo-articular tuberculosis, as these three diseases are of high and almost equivalent frequency in the country. Clinical, biological and radiological features are the same as observed in Europe. Evolution is often severe as a consequence of requently delayed diagnosis and difficulties encountered in treatment of out-patients.", "contents": "[Rheumatoid polyarthritis in Madagascar (author's transl)]. Report of 11 cases of rheumatoid arthritis observed in Malagasy. Frequently, a single joint is concerned at the on-set period and this may raise some questions to differetiate rheumatic fever, gout and osteo-articular tuberculosis, as these three diseases are of high and almost equivalent frequency in the country. Clinical, biological and radiological features are the same as observed in Europe. Evolution is often severe as a consequence of requently delayed diagnosis and difficulties encountered in treatment of out-patients."}
{"id": "PMID:481182", "title": "[Cancerized phagedenic ulcers. A study about 19 cases observed in Dakar \"H\u00f4pital Principal\" from 1961 to 1976 (author's transl)].", "content": "Cancerization is the main complication of phagedenic ulcers which are frequent in tropical areas. The detection of the turn towards cancer is generally too late because of social and psychological conditions. Patients of this study were civil servants and had beneficial of a comparatively early detection. The clinical, radiological and pathological features are described and the importance of the control of lymph nodes is emphasized. The surgical tactic is discussed according to each situation but it must be as conservative as possible, even if the amputation is only delayed since lymphatic dissemination is late and amputation eminently dramatic in the african context.", "contents": "[Cancerized phagedenic ulcers. A study about 19 cases observed in Dakar \"H\u00f4pital Principal\" from 1961 to 1976 (author's transl)]. Cancerization is the main complication of phagedenic ulcers which are frequent in tropical areas. The detection of the turn towards cancer is generally too late because of social and psychological conditions. Patients of this study were civil servants and had beneficial of a comparatively early detection. The clinical, radiological and pathological features are described and the importance of the control of lymph nodes is emphasized. The surgical tactic is discussed according to each situation but it must be as conservative as possible, even if the amputation is only delayed since lymphatic dissemination is late and amputation eminently dramatic in the african context."}
{"id": "PMID:481184", "title": "[Reaginic allergy to mold in Djibouti].", "content": "16,7 P. 100 OF RESPIRATORY ALLERGIC DISEASES IN Djibouti are caused by years, as demonstrated by the utilization of M. and M2 compounds prepared by Institut Pasteur. The role of air-conditionners appears important. Cultures of dust collected from filters reveale a much greater number of positive yeast cultures than front air.", "contents": "[Reaginic allergy to mold in Djibouti]. 16,7 P. 100 OF RESPIRATORY ALLERGIC DISEASES IN Djibouti are caused by years, as demonstrated by the utilization of M. and M2 compounds prepared by Institut Pasteur. The role of air-conditionners appears important. Cultures of dust collected from filters reveale a much greater number of positive yeast cultures than front air."}
{"id": "PMID:481185", "title": "[Retinopathy due to amino-4-quinoleines group of the synthetic antimalarial drugs (author's transl)].", "content": "The use of overdoses of amino-4-quinoleines in non-malarial diseases has demonstrated various toxic actions on eyes. The most severe is retina lesion. Metabolism of these drugs and pathogeny of the lesions are discussed. With regard to aetiology, the main factor is overdose and duration of treatment. Clinical aspects are classified in three phases; the third one is divided in 5 stages. Treatment is only prophylactic.", "contents": "[Retinopathy due to amino-4-quinoleines group of the synthetic antimalarial drugs (author's transl)]. The use of overdoses of amino-4-quinoleines in non-malarial diseases has demonstrated various toxic actions on eyes. The most severe is retina lesion. Metabolism of these drugs and pathogeny of the lesions are discussed. With regard to aetiology, the main factor is overdose and duration of treatment. Clinical aspects are classified in three phases; the third one is divided in 5 stages. Treatment is only prophylactic."}
{"id": "PMID:481183", "title": "[Basophilles degranulation test. Its value in an overseas allergologic dispensary (author's transl)].", "content": "Specific Ig.E fixed on basophilic leucocytes and mastocytes give way to a release of cytoplasmic granulations when antigen is present. This is the base of a clinical laboratory test easy to perform and consequently of great value in tropical areas. Report of first results in Republic of Djibouti.", "contents": "[Basophilles degranulation test. Its value in an overseas allergologic dispensary (author's transl)]. Specific Ig.E fixed on basophilic leucocytes and mastocytes give way to a release of cytoplasmic granulations when antigen is present. This is the base of a clinical laboratory test easy to perform and consequently of great value in tropical areas. Report of first results in Republic of Djibouti."}
{"id": "PMID:481187", "title": "[Cerebral malaria with renal insufficiency in a 5 months pregnant woman. The use of prostaglandines for delivery (author's transl)].", "content": "Report of a typical case of cerebral malaria with coma during 3 days, pneumopathy and renal insufficiency with failure of concentration. The delivery of a dead foetus has been started by prostaglandines.", "contents": "[Cerebral malaria with renal insufficiency in a 5 months pregnant woman. The use of prostaglandines for delivery (author's transl)]. Report of a typical case of cerebral malaria with coma during 3 days, pneumopathy and renal insufficiency with failure of concentration. The delivery of a dead foetus has been started by prostaglandines."}
{"id": "PMID:481186", "title": "[Test tube technique for field diagnosis of hemoglobin S (author's transl)].", "content": "Laboratory diagnosis of sickle-cell anemia requires expensive equipment often too sophisticated for rural dispensaries. Therefore haemoglobin precipitation in solutions of neutral salts appears to be a specific and reliable test, as valid as Emmel test, and easily performed during mass campaigns or within the technical and financial limitation of rural medicine.", "contents": "[Test tube technique for field diagnosis of hemoglobin S (author's transl)]. Laboratory diagnosis of sickle-cell anemia requires expensive equipment often too sophisticated for rural dispensaries. Therefore haemoglobin precipitation in solutions of neutral salts appears to be a specific and reliable test, as valid as Emmel test, and easily performed during mass campaigns or within the technical and financial limitation of rural medicine."}
{"id": "PMID:481189", "title": "[Loco-regional anesthesia in remote medical units: II.--Anesthesia of the upper limb: plexic blocks (author's transl)].", "content": "The anesthetic block of the upper limb plexus may be realized by either sus-cladications and possible complications.", "contents": "[Loco-regional anesthesia in remote medical units: II.--Anesthesia of the upper limb: plexic blocks (author's transl)]. The anesthetic block of the upper limb plexus may be realized by either sus-cladications and possible complications."}
{"id": "PMID:481188", "title": "[Diagnosis of jaundices in tropical practice (author's transl)].", "content": "Diagnosis of jaundices in tropical medicine complies with the same rules as in Europe. Some etiologies occur more frequently than in Europe; others, specific to tropical areas require peculiar investigations. Most jaundices observed overseas are hemolytic or hepatitic. Their diagnosis is generally easy. However, chronic viral hepatitis, a frequent infection, requires liver needle-biopsy for a valid diagnosis. The diagnosis of cholestatic jaundice must be conducted in a systematic fashion: if abdominal echotomography is not available, laparoscopy with or without liver needle-biopsy and percutaneous lateral transhepatic cholangiography (OKU-DA's technique) are easily performed in tropical countries and give a certain diagnosis.", "contents": "[Diagnosis of jaundices in tropical practice (author's transl)]. Diagnosis of jaundices in tropical medicine complies with the same rules as in Europe. Some etiologies occur more frequently than in Europe; others, specific to tropical areas require peculiar investigations. Most jaundices observed overseas are hemolytic or hepatitic. Their diagnosis is generally easy. However, chronic viral hepatitis, a frequent infection, requires liver needle-biopsy for a valid diagnosis. The diagnosis of cholestatic jaundice must be conducted in a systematic fashion: if abdominal echotomography is not available, laparoscopy with or without liver needle-biopsy and percutaneous lateral transhepatic cholangiography (OKU-DA's technique) are easily performed in tropical countries and give a certain diagnosis."}
{"id": "PMID:481194", "title": "Adult hypophosphatasia. Clinical, laboratory, and genetic investigation of a large kindred with review of the literature.", "content": "Investigation of the kindred of a 58-year-old woman with all of the features of \"adult\" hypophosphatasia revealed 12 individuals in 3 generations with subnormal circulating total alkaline phosphatase (AP) activity. The pattern of inheritance suggested autosomal dominant transmission, with incomplete penetrance of the trait particularly in the young males. Hypophosphatasic individuals other than the proposita were clinically well but had loss of permanent teeth, showing that dental abnormalities could be the only clinical manifestation of the disorder. Radiographic investigation of the proposita revealed that completion of stress fractures was necessary for healing; maturation of incomplete fractures resulted in stable Looser zones. Skeletal survey and radionuclide bone imaging were unremarkable in hypophosphatasic individuals without fracture. Subclinical osteopenia was found in several affected women by metacarpal cortical width and bone densitometric measurements. Laboratory studies showed increased plasma and urinary phosphoethanolamine levels in affected individuals. Phosphoethanolamine and phosphoserine appeared to be natural subtrates for AP since a negative correlation existed between each substrate and circulating total AP activity. Phosphoethanolamine and phosphoserine levels were greatest in the clinically affected proposita; furthermore, only she showed absence of leukocyte AP activity. Heat fractionation of her total circulating AP activity suggested severe reduction in the bone isoenzyme. Hypophosphatasic children had higher levels of total circulating AP than affected adults; the increase was apparently secondary to increased bone isoenzyme. Iliac crest bone biopsies showed greater abnormality in affected women. Osteoidosis was particularly pronounced in the proposita's younger affected sister and hypophosphatasic daughter. Histomorphometric analyses of the biopsies revealed a paucity of osteoblasts despite increased quantities of unmineralized matrix. The finding that hypophosphatasic children in this kindred had higher circulating total AP activity than adults and were able to model their skeleton normally, together with observations that the bone biopsy in adults had a paucity of osteoblasts, suggests that some factor(s) during growth is able to induce both AP activity and osteoblast function, or, that this disorder is an \"abiotrophy\" with deficient osteoblastic formation and/or accelerated destruction in adult life.", "contents": "Adult hypophosphatasia. Clinical, laboratory, and genetic investigation of a large kindred with review of the literature. Investigation of the kindred of a 58-year-old woman with all of the features of \"adult\" hypophosphatasia revealed 12 individuals in 3 generations with subnormal circulating total alkaline phosphatase (AP) activity. The pattern of inheritance suggested autosomal dominant transmission, with incomplete penetrance of the trait particularly in the young males. Hypophosphatasic individuals other than the proposita were clinically well but had loss of permanent teeth, showing that dental abnormalities could be the only clinical manifestation of the disorder. Radiographic investigation of the proposita revealed that completion of stress fractures was necessary for healing; maturation of incomplete fractures resulted in stable Looser zones. Skeletal survey and radionuclide bone imaging were unremarkable in hypophosphatasic individuals without fracture. Subclinical osteopenia was found in several affected women by metacarpal cortical width and bone densitometric measurements. Laboratory studies showed increased plasma and urinary phosphoethanolamine levels in affected individuals. Phosphoethanolamine and phosphoserine appeared to be natural subtrates for AP since a negative correlation existed between each substrate and circulating total AP activity. Phosphoethanolamine and phosphoserine levels were greatest in the clinically affected proposita; furthermore, only she showed absence of leukocyte AP activity. Heat fractionation of her total circulating AP activity suggested severe reduction in the bone isoenzyme. Hypophosphatasic children had higher levels of total circulating AP than affected adults; the increase was apparently secondary to increased bone isoenzyme. Iliac crest bone biopsies showed greater abnormality in affected women. Osteoidosis was particularly pronounced in the proposita's younger affected sister and hypophosphatasic daughter. Histomorphometric analyses of the biopsies revealed a paucity of osteoblasts despite increased quantities of unmineralized matrix. The finding that hypophosphatasic children in this kindred had higher circulating total AP activity than adults and were able to model their skeleton normally, together with observations that the bone biopsy in adults had a paucity of osteoblasts, suggests that some factor(s) during growth is able to induce both AP activity and osteoblast function, or, that this disorder is an \"abiotrophy\" with deficient osteoblastic formation and/or accelerated destruction in adult life."}
{"id": "PMID:481195", "title": "The morphology of \"acute tubular necrosis\" in man: analysis of 57 renal biopsies and a comparison with the glycerol model.", "content": "Renal biopsies from 24 patients with oliguric \"acute tubular necrosis\" (ATN) and 26 patients with non-oliguric ATN were compared with biopsies from 7 patients who had recently recovered from ATN and 20 control patients. Many morphologic changes were present in the biopsies of patients with ATN and absent in controls, but only two lesions were significantly more severe in patients who had ATN at the time of the biopsy compared with patients who had recently recovered from ATN. These two lesions, necrosis of individual tubular epithelial cells and loss of brush border in proximal tubules, may play a role in the pathogenesis of renal functional failure in ATN. Necrosis of individual tubular epithelial cells appeared to be a continuing process. In the patients with non-oliguric acute renal failure there was a positive correlation between duration of renal failure and severity of tubular necrosis. This was not observed in the patients with oliguric acute renal failure, but otherwise there were no identifiable morphologic differences between the two groups. The glycerol model of acute renal failure in the rabbit was found to differ in several significant ways from ATN in man. Despite the fact that the rabbits had significantly less severe renal failure, their kidneys showed much more severe tubular necrosis and much more prominent presence of tubular casts than was the case in biopsies from patients with ATN. Loss of brush border in proximal tubules was not an important feature of the glycerol model of acute renal failure in the rabbit. We suggest that the glycerol model is not analogous to human ATN and may have an entirely different pathogenesis.", "contents": "The morphology of \"acute tubular necrosis\" in man: analysis of 57 renal biopsies and a comparison with the glycerol model. Renal biopsies from 24 patients with oliguric \"acute tubular necrosis\" (ATN) and 26 patients with non-oliguric ATN were compared with biopsies from 7 patients who had recently recovered from ATN and 20 control patients. Many morphologic changes were present in the biopsies of patients with ATN and absent in controls, but only two lesions were significantly more severe in patients who had ATN at the time of the biopsy compared with patients who had recently recovered from ATN. These two lesions, necrosis of individual tubular epithelial cells and loss of brush border in proximal tubules, may play a role in the pathogenesis of renal functional failure in ATN. Necrosis of individual tubular epithelial cells appeared to be a continuing process. In the patients with non-oliguric acute renal failure there was a positive correlation between duration of renal failure and severity of tubular necrosis. This was not observed in the patients with oliguric acute renal failure, but otherwise there were no identifiable morphologic differences between the two groups. The glycerol model of acute renal failure in the rabbit was found to differ in several significant ways from ATN in man. Despite the fact that the rabbits had significantly less severe renal failure, their kidneys showed much more severe tubular necrosis and much more prominent presence of tubular casts than was the case in biopsies from patients with ATN. Loss of brush border in proximal tubules was not an important feature of the glycerol model of acute renal failure in the rabbit. We suggest that the glycerol model is not analogous to human ATN and may have an entirely different pathogenesis."}
{"id": "PMID:481197", "title": "[Evaluation of acute and subacute toxic effects of polactine yellow G].", "content": "In acute experiment the following results were obtained: the DL50 value after intragastric administration -- 8.2 g/kg of body weight, after intraperitoneal administration -- 1.33 g/kg of body weight. In the studies on acute effect irritating skin and eye of a rabbit -- only a slight inflammatory reaction in conjunctivae was found. Studies on sensitizing effect carried out on guinea pigs did not reveal any symptoms which would give evidence of an alergic effect of the dye. Lima's test did not reveal any cumulative effects of polactine G Yellow, 8-weeks experiment on 30 male rats, administered with 0.5 g/kg and 1.6 g/kg, gave the following results: in the group of rats exposed to 1.6 g/kg an increased excretion of phenol red with urine, lowered activity of aminotranspherases (AspAT and A1AT), increased activity of lactic dehydrogenase and alkaline phosphatase in the blood serum and increased relative liver weight and relative and absolute weight of the kidney. In the group of rats exposed to 0.5 g/kg of polactine yellow G, increased alkaline phosphatase in blood serum and increased relative and absolute liver weight was found. In histopathological studies in acute and subacute experiment, a damage to gastric wall was found. In addition, an increased damage to parenchymatous organs, of reversible degeneration nature, was found.", "contents": "[Evaluation of acute and subacute toxic effects of polactine yellow G]. In acute experiment the following results were obtained: the DL50 value after intragastric administration -- 8.2 g/kg of body weight, after intraperitoneal administration -- 1.33 g/kg of body weight. In the studies on acute effect irritating skin and eye of a rabbit -- only a slight inflammatory reaction in conjunctivae was found. Studies on sensitizing effect carried out on guinea pigs did not reveal any symptoms which would give evidence of an alergic effect of the dye. Lima's test did not reveal any cumulative effects of polactine G Yellow, 8-weeks experiment on 30 male rats, administered with 0.5 g/kg and 1.6 g/kg, gave the following results: in the group of rats exposed to 1.6 g/kg an increased excretion of phenol red with urine, lowered activity of aminotranspherases (AspAT and A1AT), increased activity of lactic dehydrogenase and alkaline phosphatase in the blood serum and increased relative liver weight and relative and absolute weight of the kidney. In the group of rats exposed to 0.5 g/kg of polactine yellow G, increased alkaline phosphatase in blood serum and increased relative and absolute liver weight was found. In histopathological studies in acute and subacute experiment, a damage to gastric wall was found. In addition, an increased damage to parenchymatous organs, of reversible degeneration nature, was found."}
{"id": "PMID:481198", "title": "[Gas chromatographic determination of acetic acid esters in the air].", "content": "A gas chromatographic method for determination of methyl acetate, ethyl acetate, propyl acetate, butyl acetate and amyl acetate has been elaborated. The best separation of determined compounds has been achieved on a column containing 10% Carbowax 20 M on Chromosorb W AW DMCS. Air samples are collected by passing the examined air through charcoal tubes and consequent desorption of adsorbed compounds with carbon disulfide. Activated charcoal used in all tests has been found to adsorb 10%, by weight, of acetic acid esters.", "contents": "[Gas chromatographic determination of acetic acid esters in the air]. A gas chromatographic method for determination of methyl acetate, ethyl acetate, propyl acetate, butyl acetate and amyl acetate has been elaborated. The best separation of determined compounds has been achieved on a column containing 10% Carbowax 20 M on Chromosorb W AW DMCS. Air samples are collected by passing the examined air through charcoal tubes and consequent desorption of adsorbed compounds with carbon disulfide. Activated charcoal used in all tests has been found to adsorb 10%, by weight, of acetic acid esters."}
{"id": "PMID:481199", "title": "[Method for the determination of alpha radiation potential energy of radon decay products occurring as free ions].", "content": "The paper presents a simple method of measuring the concentration of alpha radiation potential energy of radon decay products occurring as free ions in the air. This method consists in deposition of free ions of radon decay products on the surface of metal plates in so called impactor, which is constructed by an appropriate selection of shapes and mutual arrangement of the plates. The paper presents an improved system of calibration of impactor, which continuously produces free ions in the tube, through which the filtered air containing radon is being passed. The least concentration of alpha radiation potential energy of radon decay products free ions, detactable by this method, when 1 liter of air is passed through the impactor, is 10(3) MeV liter-1). This method may be applied in laboratory and environmental measurements.", "contents": "[Method for the determination of alpha radiation potential energy of radon decay products occurring as free ions]. The paper presents a simple method of measuring the concentration of alpha radiation potential energy of radon decay products occurring as free ions in the air. This method consists in deposition of free ions of radon decay products on the surface of metal plates in so called impactor, which is constructed by an appropriate selection of shapes and mutual arrangement of the plates. The paper presents an improved system of calibration of impactor, which continuously produces free ions in the tube, through which the filtered air containing radon is being passed. The least concentration of alpha radiation potential energy of radon decay products free ions, detactable by this method, when 1 liter of air is passed through the impactor, is 10(3) MeV liter-1). This method may be applied in laboratory and environmental measurements."}
{"id": "PMID:481200", "title": "[Contemporary problems of industrial toxicology].", "content": "The author presents the present -- day position and developmental trends of industrial toxicology studies, relating this specialty to other hygiene branches, especially to natural environment protection. Disproportions in the development of this line of studies was indicated, as opposed to the progress in technique and technology. These disproportions are most evident when we compare the number of chemicals for whose some data determining their biological effects have been collected, with over 4 mln. chemical compounds used in our economy. The main general problems to be solved in the nearest future are: scientific and technical information and establishment, at an international level, of basic definitions, criteria of toxicity evaluation and occupational risk, related classification and bases constituting a starting point for establishing the hygienic standards (MAC). In addition, current orientation of studies in narrower specialties within industrial toxicology was discussed, i.e. experimental toxicology, chemical air analysis, toxicological biochemistry, chemical substances metabolism in organism, pathology of acute and chronic intoxications. The development of new narrow specialties and new problems was pointed out, in view of their perspective significance for the health protection of workers occupationally exposed to chemicals. Among those problems are: remote effects of exposure to chemicals, especially the problem of industiral carcinogenesis, combined effects of poisons, and so called behavioral toxicology. It was noted that the present knowledge of carcinogenic effects of chemicals permits to gradually set up efficient preventive measures, conditioned by implementation of systems of occupational risk control and the control of health effects of exposure.", "contents": "[Contemporary problems of industrial toxicology]. The author presents the present -- day position and developmental trends of industrial toxicology studies, relating this specialty to other hygiene branches, especially to natural environment protection. Disproportions in the development of this line of studies was indicated, as opposed to the progress in technique and technology. These disproportions are most evident when we compare the number of chemicals for whose some data determining their biological effects have been collected, with over 4 mln. chemical compounds used in our economy. The main general problems to be solved in the nearest future are: scientific and technical information and establishment, at an international level, of basic definitions, criteria of toxicity evaluation and occupational risk, related classification and bases constituting a starting point for establishing the hygienic standards (MAC). In addition, current orientation of studies in narrower specialties within industrial toxicology was discussed, i.e. experimental toxicology, chemical air analysis, toxicological biochemistry, chemical substances metabolism in organism, pathology of acute and chronic intoxications. The development of new narrow specialties and new problems was pointed out, in view of their perspective significance for the health protection of workers occupationally exposed to chemicals. Among those problems are: remote effects of exposure to chemicals, especially the problem of industiral carcinogenesis, combined effects of poisons, and so called behavioral toxicology. It was noted that the present knowledge of carcinogenic effects of chemicals permits to gradually set up efficient preventive measures, conditioned by implementation of systems of occupational risk control and the control of health effects of exposure."}
{"id": "PMID:481201", "title": "[Spinal pain syndromes in coal miners].", "content": "The paper presents an evaluation of spinal pain syndrome prevalence in miners of a Mining Industry Corporation, employing 45 thous of manual workers. The observed population included 34.487 underground workers, and 9.507 surface workers. The distribution of workplaces was based on the degree of the spine load. The number of examined sick workers during one year amounted to 1.600. The authors noted, that the morbidity among underground workers with considerably loaded spine is 1/3 higher and they begin to complain about 5 years earlier than surface workers -- which obviously results from the type and environment of the work. On the other hand no difference was found in the morbidity between underground and surface workers engaged in the work with a middle load of spine, because the type of work is similar in this case and the effects of environment are not significant.", "contents": "[Spinal pain syndromes in coal miners]. The paper presents an evaluation of spinal pain syndrome prevalence in miners of a Mining Industry Corporation, employing 45 thous of manual workers. The observed population included 34.487 underground workers, and 9.507 surface workers. The distribution of workplaces was based on the degree of the spine load. The number of examined sick workers during one year amounted to 1.600. The authors noted, that the morbidity among underground workers with considerably loaded spine is 1/3 higher and they begin to complain about 5 years earlier than surface workers -- which obviously results from the type and environment of the work. On the other hand no difference was found in the morbidity between underground and surface workers engaged in the work with a middle load of spine, because the type of work is similar in this case and the effects of environment are not significant."}
{"id": "PMID:481202", "title": "[Thermographic functional test in the diagnosis of vascular changes in the vibration syndrome].", "content": "Contactant thermometry of fingers' skin in thermal functional test is a routine examination common now in vascular changes diagnosis. The sensitivity of this test is increased by the use of a linear thermograph, averaging the temperature of the skin area being examined. In order to ensure correct measurement results, conditions and principles of the test performance must be observed carefully, as indicated by the authors. Evaluation of time thermograms of fingers' skin should involve three types of thermoregulating reaction: homoiothermic, poikilothermic and amphithermic. The first type may be numbered among absolute, whereas the other two among relative physiological standards. Evaluation should include: fingers' skin temperature before cooling, the rate of temperature rise between the 1st and the 5th minute after cooling and the temperature taken at the 15th minute after cooling. Repeated measurements allow to differentiate changes in fingers' skin temperature, resulting from a damage to vessels due to vibration, as well as a relative physiological standard.", "contents": "[Thermographic functional test in the diagnosis of vascular changes in the vibration syndrome]. Contactant thermometry of fingers' skin in thermal functional test is a routine examination common now in vascular changes diagnosis. The sensitivity of this test is increased by the use of a linear thermograph, averaging the temperature of the skin area being examined. In order to ensure correct measurement results, conditions and principles of the test performance must be observed carefully, as indicated by the authors. Evaluation of time thermograms of fingers' skin should involve three types of thermoregulating reaction: homoiothermic, poikilothermic and amphithermic. The first type may be numbered among absolute, whereas the other two among relative physiological standards. Evaluation should include: fingers' skin temperature before cooling, the rate of temperature rise between the 1st and the 5th minute after cooling and the temperature taken at the 15th minute after cooling. Repeated measurements allow to differentiate changes in fingers' skin temperature, resulting from a damage to vessels due to vibration, as well as a relative physiological standard."}
{"id": "PMID:481203", "title": "[Ear protective plugs].", "content": "The authors present results of studies of application of polyurethane foam to produce individual sound-absorbing ear plugs. In addition to physico-mechanical properties of the foam, the authors present its optimum prescribed composition, ensuring appropriate attenuation qualities, flexibility, softness and long -- lasting strain relaxation -- i.e. the conditions resulting in excellent filling of external auditory meatus, masking effect and utilization comfort. The efficiency of attenuation of sounds by the plugs, tested audiometrically by the method of auditory threshold shift in otologically healthy subjects ranges from 12.25 dB (250 Hz) to 29.75 dB (4000 Hz). No significant inflammatory reactions in external ear skin were found after 8 hrs of continuous wearing of ranges under the work conditions typical for the textile industry. The results demonstrate that the polyurethane foam, developed by the authors, are valuable ear protectors, of advantageous functional qualities.", "contents": "[Ear protective plugs]. The authors present results of studies of application of polyurethane foam to produce individual sound-absorbing ear plugs. In addition to physico-mechanical properties of the foam, the authors present its optimum prescribed composition, ensuring appropriate attenuation qualities, flexibility, softness and long -- lasting strain relaxation -- i.e. the conditions resulting in excellent filling of external auditory meatus, masking effect and utilization comfort. The efficiency of attenuation of sounds by the plugs, tested audiometrically by the method of auditory threshold shift in otologically healthy subjects ranges from 12.25 dB (250 Hz) to 29.75 dB (4000 Hz). No significant inflammatory reactions in external ear skin were found after 8 hrs of continuous wearing of ranges under the work conditions typical for the textile industry. The results demonstrate that the polyurethane foam, developed by the authors, are valuable ear protectors, of advantageous functional qualities."}
{"id": "PMID:481213", "title": "Influence of acetylsalicylic acid on plasma glucose, insulin, glucagon, and growth hormone levels following tolbutamide stimulation in man.", "content": "The effects of acetylsalicylic acid (ASA), a known inhibitor of prostaglandin (PG) synthesis, on plasma glucose, insulin, glucagon and growth hormone (GH) responses to tolbutamide were examined in ten normal volunteers. Treatment with 3.2 g ASA daily for 3 days caused a significant reduction in basal plasma glucose levels (p less than 0.05); by contrast, basal insulin rose from 23 +/- 2 to 31 +/- 2 microU/ml (p less than 0.01). No significant changes in the basal concentrations of glucagon and GH were found after ASA. Insulin response to tolbutamide was significantly augmented after ASA (p less than 0.01) while GH response to hypoglycemia was reduced (p less than 0.05). The pattern of plasma glucose and glucagon was not significantly modified by the treatment. Since ASA seems to have an action opposite to PGE on insulin and GH secretion, it is possible that the ASA may work through inhibition of PG synthesis.", "contents": "Influence of acetylsalicylic acid on plasma glucose, insulin, glucagon, and growth hormone levels following tolbutamide stimulation in man. The effects of acetylsalicylic acid (ASA), a known inhibitor of prostaglandin (PG) synthesis, on plasma glucose, insulin, glucagon and growth hormone (GH) responses to tolbutamide were examined in ten normal volunteers. Treatment with 3.2 g ASA daily for 3 days caused a significant reduction in basal plasma glucose levels (p less than 0.05); by contrast, basal insulin rose from 23 +/- 2 to 31 +/- 2 microU/ml (p less than 0.01). No significant changes in the basal concentrations of glucagon and GH were found after ASA. Insulin response to tolbutamide was significantly augmented after ASA (p less than 0.01) while GH response to hypoglycemia was reduced (p less than 0.05). The pattern of plasma glucose and glucagon was not significantly modified by the treatment. Since ASA seems to have an action opposite to PGE on insulin and GH secretion, it is possible that the ASA may work through inhibition of PG synthesis."}
{"id": "PMID:481215", "title": "Abnormal lipoprotein-lipase-mediated plasma triglyceride removal in untreated diabetes mellitus associated with hypertriglyceridemia.", "content": "Hypertriglyceridemia is common in untreated diabetes mellitus. An abnormality in the interaction of lipoprotein lipase with endogenous circulating plasma lipoprotein triglyceride has been demonstrated in untreated diabetes. These diabetics have a decreased maximal removal capacity for plasma triglyceride (27.0 mg TG/kh/hr) and increased Km (390 mg/dl) for endogenous plasma triglyceride-lipoprotein lipase interaction compared to that found in nondiabetic hypertriglyceridemic subjects (Vmax, 32.0; km, 157). Diabetics treated for at least two months have a maximal removal capacity and Km similar to that of nondiabetic subjects (Vmax, 32.7; Km, 192). No evidence for an increase in triglyceride production due to diabetes per se was found. When diabetic subjects with triglyceride levels over 400 mg/dl were selected for study, most were found to have an independent familial form of hypertriglyceridemia.", "contents": "Abnormal lipoprotein-lipase-mediated plasma triglyceride removal in untreated diabetes mellitus associated with hypertriglyceridemia. Hypertriglyceridemia is common in untreated diabetes mellitus. An abnormality in the interaction of lipoprotein lipase with endogenous circulating plasma lipoprotein triglyceride has been demonstrated in untreated diabetes. These diabetics have a decreased maximal removal capacity for plasma triglyceride (27.0 mg TG/kh/hr) and increased Km (390 mg/dl) for endogenous plasma triglyceride-lipoprotein lipase interaction compared to that found in nondiabetic hypertriglyceridemic subjects (Vmax, 32.0; km, 157). Diabetics treated for at least two months have a maximal removal capacity and Km similar to that of nondiabetic subjects (Vmax, 32.7; Km, 192). No evidence for an increase in triglyceride production due to diabetes per se was found. When diabetic subjects with triglyceride levels over 400 mg/dl were selected for study, most were found to have an independent familial form of hypertriglyceridemia."}
{"id": "PMID:481218", "title": "Failure to demonstrate stimulatory effect of prolactin on vitamin D metabolism in vitamin-D-deficient rats.", "content": "The effect of administration of bovine prolactin in vivo on renal 1 alpha-hydroxylase (1 alpha-OHase) activity was investigated using thyroparathyroidectomized and sham-operated vitamin-D-deficient male rats. A high dose (1.5 IU/hr) and a low dose (0.3 IU/hr) of bovine prolactin was constantly infused into the cannulated femoral vein for 6 hr or 30 hr. The accumulation of 1 alpha, 25-hydroxyvitamin D3 [1 alpha, 25-(OH)2-D3] in plasma produced from 25-hydroxy-vitamin D3 (25-OH-D3) was calculated from the 6-hr conversion of intravenously injected [3H]-25-OH-D3 to [3H]-1 alpha, 25-(OH)2-D3. Both high dose and low dose of prolactin administration caused no significant stimulatory effect on 1 alpha-OHase activity in TPTX animals. Also, in sham-operated rats the prolactin treatment produced no significant change in the 1 alpha-OHase activity. Plasma calcium concentrations showed a tendency to increase slightly. These results suggest that prolactin has no direct stimulatory role on renal 1 alpha-OHase activity in rats. Its effect on calcium metabolism may be mediated by a different mechanism(s).", "contents": "Failure to demonstrate stimulatory effect of prolactin on vitamin D metabolism in vitamin-D-deficient rats. The effect of administration of bovine prolactin in vivo on renal 1 alpha-hydroxylase (1 alpha-OHase) activity was investigated using thyroparathyroidectomized and sham-operated vitamin-D-deficient male rats. A high dose (1.5 IU/hr) and a low dose (0.3 IU/hr) of bovine prolactin was constantly infused into the cannulated femoral vein for 6 hr or 30 hr. The accumulation of 1 alpha, 25-hydroxyvitamin D3 [1 alpha, 25-(OH)2-D3] in plasma produced from 25-hydroxy-vitamin D3 (25-OH-D3) was calculated from the 6-hr conversion of intravenously injected [3H]-25-OH-D3 to [3H]-1 alpha, 25-(OH)2-D3. Both high dose and low dose of prolactin administration caused no significant stimulatory effect on 1 alpha-OHase activity in TPTX animals. Also, in sham-operated rats the prolactin treatment produced no significant change in the 1 alpha-OHase activity. Plasma calcium concentrations showed a tendency to increase slightly. These results suggest that prolactin has no direct stimulatory role on renal 1 alpha-OHase activity in rats. Its effect on calcium metabolism may be mediated by a different mechanism(s)."}
{"id": "PMID:481220", "title": "Impact of hemodialysis on the abnormal glucose and alanine kinetics of chronic azotemia.", "content": "Rates of alanine and glucose turnover and precursor-product interrelationships were determined in patients on chronic hemodialysis and in matched controls using simultaneous primed injection-continuous infusions of [U-14C] alanine and [2-3h] glucose. In eight chronically dialyzed patients studied before their first dialysis of the week, glucose turnover was 866 +/- 120 micromole/min (mean +/- SE); after their last dialysis of the week, glucose turnover was 880 +/- 63 micromole/min. These rates were 35% (p less than 0.05) and 37% (p less than 0.01) greater than rates observed in ten normal volunteers (642 +/- 28.3 micromole/min). Fasting glucose and insulin levels in dialyzing patients were unchanged from normal. Alanine turnover was increased predialysis (318 +/- 55.2 micromoles/min; p less than 0.01) and postdialysis (248 +/- 32.4 micromole/min; p less than 0.01) as compared to normal (168 +/- 14.3 micromole/min). In patients pre- and postdialysis, gluconeogenesis from alanine was increased to 34.6 +/- 10.9 micromole/min (p less than 0.05) and 39.0 +/- 6.33 micromole/min (p less than 0.05) compared to 20.9 +/- 1.63 micromole/min in normal subjects. We conclude that neither acute nor chronic hemodialysis corrects the increased glucose and alanine production and utilization and gluconeogenesis observed in chronic renal failure.", "contents": "Impact of hemodialysis on the abnormal glucose and alanine kinetics of chronic azotemia. Rates of alanine and glucose turnover and precursor-product interrelationships were determined in patients on chronic hemodialysis and in matched controls using simultaneous primed injection-continuous infusions of [U-14C] alanine and [2-3h] glucose. In eight chronically dialyzed patients studied before their first dialysis of the week, glucose turnover was 866 +/- 120 micromole/min (mean +/- SE); after their last dialysis of the week, glucose turnover was 880 +/- 63 micromole/min. These rates were 35% (p less than 0.05) and 37% (p less than 0.01) greater than rates observed in ten normal volunteers (642 +/- 28.3 micromole/min). Fasting glucose and insulin levels in dialyzing patients were unchanged from normal. Alanine turnover was increased predialysis (318 +/- 55.2 micromoles/min; p less than 0.01) and postdialysis (248 +/- 32.4 micromole/min; p less than 0.01) as compared to normal (168 +/- 14.3 micromole/min). In patients pre- and postdialysis, gluconeogenesis from alanine was increased to 34.6 +/- 10.9 micromole/min (p less than 0.05) and 39.0 +/- 6.33 micromole/min (p less than 0.05) compared to 20.9 +/- 1.63 micromole/min in normal subjects. We conclude that neither acute nor chronic hemodialysis corrects the increased glucose and alanine production and utilization and gluconeogenesis observed in chronic renal failure."}
{"id": "PMID:481222", "title": "Role of endogenous somatostatin in the secretion of parathyroid hormone and calcitonin.", "content": "Our previous in vitro and in vivo studies demonstrated that exogenous somatostatin inhibited secretion of both parathyroid hormone (PTH) and calcitonin (CT). This study evaluates the possible role of endogenous somatostatin in PTH and CT secretion. Rats receiving somatostatin antiserum i.v. had significantly greater circulating levels of serum immunoreactive PTH (iPTH) and CT (iCT) than rats receiving normal rabbit serum. In in vitro studies with bovine parathyroid tissue, the addition of somatostatin antiserum to the medium significantly increased PTH secretion from basal, low calcium-stimulated and high calcium-suppressed parathyroid tissue. These combined observations strongly suggest that endogenous somatostatin must have a suppressive effect on PTH and CT secretion. The in vitro observations with isolated parathyroid tissue suggest that somatostatin is synthesized by cells within this tissue. These data strongly suggest that somatostatin is a locally-synthesized hormone that has a role in modulation of both PTH and CT secretion.", "contents": "Role of endogenous somatostatin in the secretion of parathyroid hormone and calcitonin. Our previous in vitro and in vivo studies demonstrated that exogenous somatostatin inhibited secretion of both parathyroid hormone (PTH) and calcitonin (CT). This study evaluates the possible role of endogenous somatostatin in PTH and CT secretion. Rats receiving somatostatin antiserum i.v. had significantly greater circulating levels of serum immunoreactive PTH (iPTH) and CT (iCT) than rats receiving normal rabbit serum. In in vitro studies with bovine parathyroid tissue, the addition of somatostatin antiserum to the medium significantly increased PTH secretion from basal, low calcium-stimulated and high calcium-suppressed parathyroid tissue. These combined observations strongly suggest that endogenous somatostatin must have a suppressive effect on PTH and CT secretion. The in vitro observations with isolated parathyroid tissue suggest that somatostatin is synthesized by cells within this tissue. These data strongly suggest that somatostatin is a locally-synthesized hormone that has a role in modulation of both PTH and CT secretion."}
{"id": "PMID:481250", "title": "Difficult intubations.", "content": "Six cases of difficult intubation are presented and their management discussed. Routine practice of blind nasal intubation and fiberoptic laryngoscopy is recommended for improved dexterity. Elective tracheostomy should be considered in cases where the airway is compromised and cricothyroidotomy should be recommended.", "contents": "Difficult intubations. Six cases of difficult intubation are presented and their management discussed. Routine practice of blind nasal intubation and fiberoptic laryngoscopy is recommended for improved dexterity. Elective tracheostomy should be considered in cases where the airway is compromised and cricothyroidotomy should be recommended."}
{"id": "PMID:481251", "title": "The treatment of severe pancreatic pain.", "content": "The management of four patients with severe pancreatic pain is discussed. Bilateral coeliac plexus block with alcohol gave effective pain relief to the three patients with pancreatic carcinoma and the one patient with acute pancreatitis. The treatment of the pain of acute and chronic pancreatitis is reviewed.", "contents": "The treatment of severe pancreatic pain. The management of four patients with severe pancreatic pain is discussed. Bilateral coeliac plexus block with alcohol gave effective pain relief to the three patients with pancreatic carcinoma and the one patient with acute pancreatitis. The treatment of the pain of acute and chronic pancreatitis is reviewed."}
{"id": "PMID:481258", "title": "Biochemical characterization of H2S-positive Salmonella sendai strains isolated in Hong Kong.", "content": "The biochemical properties of 8 H2S-positive variant strains of Salmonella sendai isolated from patients and carriers in Hong Kong were studied. Apart from the production of H2S, all these strains showed typical properties of S. sendai and a dependence on tryptophan. Their capacity to utilize different forms of sulphur sources varied, ranging from being capable of utilizing SO4, SO3, S2O3 and cystein to only cystein as the sulphur source.", "contents": "Biochemical characterization of H2S-positive Salmonella sendai strains isolated in Hong Kong. The biochemical properties of 8 H2S-positive variant strains of Salmonella sendai isolated from patients and carriers in Hong Kong were studied. Apart from the production of H2S, all these strains showed typical properties of S. sendai and a dependence on tryptophan. Their capacity to utilize different forms of sulphur sources varied, ranging from being capable of utilizing SO4, SO3, S2O3 and cystein to only cystein as the sulphur source."}
{"id": "PMID:481259", "title": "UV survival of human mycoplasmas: evidence of dark reactivation in Mycoplasma buccale.", "content": "The inactivation by ultraviolet (UV) light irradiation of mycoplasma cells of five human strains was monitored by investigating the colony-forming ability. The survival curves of five strains tested indicated that the cells of Mycoplasma buccale only are single and homogenously susceptible to UV light. The effect of the repair inhibitor, caffeine, on the colony-forming ability of UV-irradiated cells was investigated with M. buccale because of its homogenous susceptibility to UV light. The colony formation of irradiated cells was markedly depressed by post-irradiation treatment with caffeine at concentrations that had little or no effect on the colony formation of unirradiated cells. The colony-forming units (CFU) of UV-irradiated cells which were kept in broth without caffeine in the dark increased without a lag as the time in the dark increased. The colony-forming ability of the irradiated cells completely recovered after 3 hr in the dark. However, when irradiated cells were kept in the presence of caffeine, no increase in their CFU was observed. The mode of action of caffeine on UV-irradiated cells closely resembles that described for other organisms which possess dark reactivation systems for UV-induced damage in deoxyribonucleic acid (DNA). Thus, the results obtained provide evidence for the existence of a dark repair function in M. buccale.", "contents": "UV survival of human mycoplasmas: evidence of dark reactivation in Mycoplasma buccale. The inactivation by ultraviolet (UV) light irradiation of mycoplasma cells of five human strains was monitored by investigating the colony-forming ability. The survival curves of five strains tested indicated that the cells of Mycoplasma buccale only are single and homogenously susceptible to UV light. The effect of the repair inhibitor, caffeine, on the colony-forming ability of UV-irradiated cells was investigated with M. buccale because of its homogenous susceptibility to UV light. The colony formation of irradiated cells was markedly depressed by post-irradiation treatment with caffeine at concentrations that had little or no effect on the colony formation of unirradiated cells. The colony-forming units (CFU) of UV-irradiated cells which were kept in broth without caffeine in the dark increased without a lag as the time in the dark increased. The colony-forming ability of the irradiated cells completely recovered after 3 hr in the dark. However, when irradiated cells were kept in the presence of caffeine, no increase in their CFU was observed. The mode of action of caffeine on UV-irradiated cells closely resembles that described for other organisms which possess dark reactivation systems for UV-induced damage in deoxyribonucleic acid (DNA). Thus, the results obtained provide evidence for the existence of a dark repair function in M. buccale."}
{"id": "PMID:481272", "title": "[Elimination of nitrous oxide by a combined bacterial culture].", "content": "The ability of bacteria to eliminate nitrous oxide (N2O) from a gaseous phase containing 20% O2 was studied. Representatives of the following physiological groups were found to be incapable of removing N2O: denitrifying bacteria (Paracoccus denitrificans), nitrifying bacteria (Nitrosospira briensis), carboxydobacteria (Pseudomonas carboxydoflava), methane oxidizing bacteria (Methylosinus trichosporium), and nitrogen fixing bacteria (Bacillus polymyxa). Five corynebacteria-like strains were isolated from soil samples actively eliminating N2O under oxidative conditions. None of the strains could remove considerable amounts of N2O in pure culture. The rate of N2O elimination was much higher than that of the initial cultures if a combined culture comprising five strains was used. Three strains of the combined culture formed H2O2 and possessed a low catalase activity whereas the remaining two did not produce H2O2 and decomposed hydrogen peroxide at a high rate; this suggests that the catalase mechanism is involved in the elimination of N2O.", "contents": "[Elimination of nitrous oxide by a combined bacterial culture]. The ability of bacteria to eliminate nitrous oxide (N2O) from a gaseous phase containing 20% O2 was studied. Representatives of the following physiological groups were found to be incapable of removing N2O: denitrifying bacteria (Paracoccus denitrificans), nitrifying bacteria (Nitrosospira briensis), carboxydobacteria (Pseudomonas carboxydoflava), methane oxidizing bacteria (Methylosinus trichosporium), and nitrogen fixing bacteria (Bacillus polymyxa). Five corynebacteria-like strains were isolated from soil samples actively eliminating N2O under oxidative conditions. None of the strains could remove considerable amounts of N2O in pure culture. The rate of N2O elimination was much higher than that of the initial cultures if a combined culture comprising five strains was used. Three strains of the combined culture formed H2O2 and possessed a low catalase activity whereas the remaining two did not produce H2O2 and decomposed hydrogen peroxide at a high rate; this suggests that the catalase mechanism is involved in the elimination of N2O."}
{"id": "PMID:481273", "title": "[Glycerin metabolism in purple sulfur bacteria].", "content": "The purple sulfur bacteria Thiocapsa roseopersicina, strains BBS and SL-4, and Lamprobacter modestohalophilus assimilate glycerol when grown in the light under the anaerobic conditions or in the dark under the aerobic conditions. Apart from glycerol, the bacteria require for their growth reduced sulfur compounds (thiosulfate) and bicarbonate. The 14C from 1-14C-glycerol is incorporated into the cells of T. roseopersicina and L. modestohalophilus at a lower rate in the presence of thiosulfate under the phototrophic conditions. In the presence of glycerol, all the studied purple sulfur bacteria produce more reserve carbohydrates of the glucan type than on the mineral medium. Glycerol metabolism in purple sulfur bacteria, like that in nonsulfur purple bacteria, involves glycerokinase and the pyridine nucleotide independent alpha-glycerophosphate dehydrogenase. The activity of these enzymes does not depend on the conditions of the cultural growth and the presence of glycerol in the medium.", "contents": "[Glycerin metabolism in purple sulfur bacteria]. The purple sulfur bacteria Thiocapsa roseopersicina, strains BBS and SL-4, and Lamprobacter modestohalophilus assimilate glycerol when grown in the light under the anaerobic conditions or in the dark under the aerobic conditions. Apart from glycerol, the bacteria require for their growth reduced sulfur compounds (thiosulfate) and bicarbonate. The 14C from 1-14C-glycerol is incorporated into the cells of T. roseopersicina and L. modestohalophilus at a lower rate in the presence of thiosulfate under the phototrophic conditions. In the presence of glycerol, all the studied purple sulfur bacteria produce more reserve carbohydrates of the glucan type than on the mineral medium. Glycerol metabolism in purple sulfur bacteria, like that in nonsulfur purple bacteria, involves glycerokinase and the pyridine nucleotide independent alpha-glycerophosphate dehydrogenase. The activity of these enzymes does not depend on the conditions of the cultural growth and the presence of glycerol in the medium."}
{"id": "PMID:481274", "title": "[Effect of formate on the respiration of different microorganisms].", "content": "The cells of methylotrophic bacteria (Achromobacter parvulus 1, Pseudomonas methylica 2 and 20, Ps. fluorescens 45, Ps. oleovorans 52) and Candida methylica 101 grown in a medium with methanol take up oxygen in the presence of formiate. A. parvulus is most resistant to formiate (KM = 2.3 +/- 0.5) X 10(-3) M; Ki = 4 x 10(-1) M). When grown on Cn substrates, these microorganisms cannot oxidize formiate (with an exception of A. parvulus). Formiate also serves as a respiration substrate for E. coli K-12 and the purple bacterium Rhodopseudomonas palustris, disregarding the composition of a medium on which the cells are grown. When the cells cannot oxidize formiate, it inhibits the respiration of the cells and cell extracts in the presence of other substrates, including TMPD + ascorbate. The respiration is completely inhibited at a concentration of formiate of 10(-3)--10(-2) M. It follows therefore that formiate acts on the terminal cytochrome oxidase, regardless of whether it is cytochrome a or o.", "contents": "[Effect of formate on the respiration of different microorganisms]. The cells of methylotrophic bacteria (Achromobacter parvulus 1, Pseudomonas methylica 2 and 20, Ps. fluorescens 45, Ps. oleovorans 52) and Candida methylica 101 grown in a medium with methanol take up oxygen in the presence of formiate. A. parvulus is most resistant to formiate (KM = 2.3 +/- 0.5) X 10(-3) M; Ki = 4 x 10(-1) M). When grown on Cn substrates, these microorganisms cannot oxidize formiate (with an exception of A. parvulus). Formiate also serves as a respiration substrate for E. coli K-12 and the purple bacterium Rhodopseudomonas palustris, disregarding the composition of a medium on which the cells are grown. When the cells cannot oxidize formiate, it inhibits the respiration of the cells and cell extracts in the presence of other substrates, including TMPD + ascorbate. The respiration is completely inhibited at a concentration of formiate of 10(-3)--10(-2) M. It follows therefore that formiate acts on the terminal cytochrome oxidase, regardless of whether it is cytochrome a or o."}
{"id": "PMID:481276", "title": "[Lipid component of Streptomyces levoris membranes].", "content": "The total composition of membranes is characterized in Streptomyces levoris producing the polyene antibiotic levorin in the course of its growth; the lipid component has been studied in detail. The membranes contained protein (50--55%), lipids (37--40%), carbohydrates (3--4%), and RNA (2--3%). The ratio between the main components hardly changed with age and only the content of total phosphorus in the membranes decreased noticeably. The lipid component was represented mainly by polar lipids (80--90%); phosphatidyl ethanolamine, phosphatidyl inositolmannoside and cardiolipin prevalied. The content of phospholipids in the fraction of polar lipids gradually decreased. The possibility of substituting phospholipids by analogues containing no phosphorus is discussed. The fatty acid spectrum of membranous lipids was represented mainly by saturated fatty acids with a branched chain of the iso and anteiso series, and changed with age only to a minor extent.", "contents": "[Lipid component of Streptomyces levoris membranes]. The total composition of membranes is characterized in Streptomyces levoris producing the polyene antibiotic levorin in the course of its growth; the lipid component has been studied in detail. The membranes contained protein (50--55%), lipids (37--40%), carbohydrates (3--4%), and RNA (2--3%). The ratio between the main components hardly changed with age and only the content of total phosphorus in the membranes decreased noticeably. The lipid component was represented mainly by polar lipids (80--90%); phosphatidyl ethanolamine, phosphatidyl inositolmannoside and cardiolipin prevalied. The content of phospholipids in the fraction of polar lipids gradually decreased. The possibility of substituting phospholipids by analogues containing no phosphorus is discussed. The fatty acid spectrum of membranous lipids was represented mainly by saturated fatty acids with a branched chain of the iso and anteiso series, and changed with age only to a minor extent."}
{"id": "PMID:481279", "title": "[Growth of a Propionibacterium shermanii culture under varying aeration conditions].", "content": "The effect of aeration of growth of Propionibacterium shermanii BKM B-103 was studied. Aeration was found to stimulate growth of the culture in a complex medium containing sodium lactate as a source of carbon and energy. When the concentration of oxygen in the medium was raised, the economic coefficient increased, the Ks value for lactate assimilation decreased, and the value of mum did not change.", "contents": "[Growth of a Propionibacterium shermanii culture under varying aeration conditions]. The effect of aeration of growth of Propionibacterium shermanii BKM B-103 was studied. Aeration was found to stimulate growth of the culture in a complex medium containing sodium lactate as a source of carbon and energy. When the concentration of oxygen in the medium was raised, the economic coefficient increased, the Ks value for lactate assimilation decreased, and the value of mum did not change."}
{"id": "PMID:481280", "title": "[Structural changes in Mycobacterium rubrum cells under the influence of chloroethylamine].", "content": "Chloroethylamine was used to differentiate the membranous structures of bacterial cells whose physiological role becomes more pronounced under the action of this substance. The following changes were found: in the synthesis of the cell wall as a result of modifications in the structure of mesosomes which control the correct orientation of growth and cell division; in the nucleoid and nucleoidosomes; in the analogues of mitochondria characterizing the level of energy metabolism and in the analogues of the endoplasmic reticulum accompanied with changes in the synthesis of metabolites and reserve substances in the cells. Changes in the structure and ultrastructure of the bacteria under the action of irradiation and the radiomimetic substance chloroethylamine are analyzed.", "contents": "[Structural changes in Mycobacterium rubrum cells under the influence of chloroethylamine]. Chloroethylamine was used to differentiate the membranous structures of bacterial cells whose physiological role becomes more pronounced under the action of this substance. The following changes were found: in the synthesis of the cell wall as a result of modifications in the structure of mesosomes which control the correct orientation of growth and cell division; in the nucleoid and nucleoidosomes; in the analogues of mitochondria characterizing the level of energy metabolism and in the analogues of the endoplasmic reticulum accompanied with changes in the synthesis of metabolites and reserve substances in the cells. Changes in the structure and ultrastructure of the bacteria under the action of irradiation and the radiomimetic substance chloroethylamine are analyzed."}
{"id": "PMID:481277", "title": "[Respiratory cyanide resistance in Candida lipolytica and the supersynthesis of citric acids].", "content": "The interrelationship between the cyanide resistance of respiratio and the process of citric acid synthesis was studied with various strains of Candida lipolytica. When the synthesis of citric acids was intensive, the respiration of cells was almost entirely inhibited by cyanide. The respiration became cyanide resistant only when the biosynthesis of citric acids was over. Induction of the cyanide resistance of respiration with antimycin A stopped the biosynthesis of citric acids. The cyanide resistance of respiration in yeast cultures incapable of considerable production of citric acids was displayed when the growth ceased. There was a strong direct correlation between the rate of citric acid synthesis and the degree of inhibition of the respiration by cyanide. It was concluded therefore that the process of citric acid overproduction could reset the reduction equivalents, thus making unnecessary the cyanide resistant pathway of electron transport.", "contents": "[Respiratory cyanide resistance in Candida lipolytica and the supersynthesis of citric acids]. The interrelationship between the cyanide resistance of respiratio and the process of citric acid synthesis was studied with various strains of Candida lipolytica. When the synthesis of citric acids was intensive, the respiration of cells was almost entirely inhibited by cyanide. The respiration became cyanide resistant only when the biosynthesis of citric acids was over. Induction of the cyanide resistance of respiration with antimycin A stopped the biosynthesis of citric acids. The cyanide resistance of respiration in yeast cultures incapable of considerable production of citric acids was displayed when the growth ceased. There was a strong direct correlation between the rate of citric acid synthesis and the degree of inhibition of the respiration by cyanide. It was concluded therefore that the process of citric acid overproduction could reset the reduction equivalents, thus making unnecessary the cyanide resistant pathway of electron transport."}
{"id": "PMID:481282", "title": "[Spheroplast study of Thiobacillus ferrooxidans].", "content": "Spheroplasts were prepared, under the action of lysozyme (200 and 400 mcg/ml) from the cells of Thiobacillus ferrooxidans grown on the Waksman medium with elementary sulfur after their treatment with 0.5M NaCl solutions and exact exposure to sucrose (0.5M). Intracellular structures were revealed on the spheroplasts which could not be detected on intact cells.", "contents": "[Spheroplast study of Thiobacillus ferrooxidans]. Spheroplasts were prepared, under the action of lysozyme (200 and 400 mcg/ml) from the cells of Thiobacillus ferrooxidans grown on the Waksman medium with elementary sulfur after their treatment with 0.5M NaCl solutions and exact exposure to sucrose (0.5M). Intracellular structures were revealed on the spheroplasts which could not be detected on intact cells."}
{"id": "PMID:481283", "title": "[Dynamics of the accumulation of adifferentiated variants in a submerged culture of Streptomyces roseoflavus var. roseofungini].", "content": "When Streptomyces roseoflavus var. roseofungini is grown as a submerged culture in a chemically defined medium with stirring, it yields a number of variants in which the capacity to form the aerial mycelium is disturbed. Inoculations of the submerged mycelium of different age into a solid medium give rise to both \"tomentose\" variants (TV) with the aerial mycelium and \"naked\" variants (NV) lacking the aerial mycelium. The latter variants comprise Nocardia-like variants (NLV) with the fragmenting mycelium. The following change in the variants occurs in the inoculations of the submerged mycelium in the course of the cultural growth: TV leads to NV leads to NLV or (more rarely) TV leads to NLV. The dynamics of accumulation of the variants depends, to a considerable extent, on the source of carbon in the medium: NLV first appear on the medium containing fructose; NV are detected first on the medium with glucose; NV are rarely found on the medium with mannitol. In aged cultures, the number of NLV can reach 80--90%, the content of TV is 20--10%, and NV are absent. The above results suggest that the formation of NLV from S. roseoflavus var. roseofungini is the result of stepwise selection.", "contents": "[Dynamics of the accumulation of adifferentiated variants in a submerged culture of Streptomyces roseoflavus var. roseofungini]. When Streptomyces roseoflavus var. roseofungini is grown as a submerged culture in a chemically defined medium with stirring, it yields a number of variants in which the capacity to form the aerial mycelium is disturbed. Inoculations of the submerged mycelium of different age into a solid medium give rise to both \"tomentose\" variants (TV) with the aerial mycelium and \"naked\" variants (NV) lacking the aerial mycelium. The latter variants comprise Nocardia-like variants (NLV) with the fragmenting mycelium. The following change in the variants occurs in the inoculations of the submerged mycelium in the course of the cultural growth: TV leads to NV leads to NLV or (more rarely) TV leads to NLV. The dynamics of accumulation of the variants depends, to a considerable extent, on the source of carbon in the medium: NLV first appear on the medium containing fructose; NV are detected first on the medium with glucose; NV are rarely found on the medium with mannitol. In aged cultures, the number of NLV can reach 80--90%, the content of TV is 20--10%, and NV are absent. The above results suggest that the formation of NLV from S. roseoflavus var. roseofungini is the result of stepwise selection."}
{"id": "PMID:481284", "title": "[Bacteriocin-like factor in Bacillus thuringiensis].", "content": "The paper describes Bacillus thuringiensis var. galleriae strains possessing a bacteriocin-like factor (factor K) which inhibits the cultures of a number of serotypes of Bac. thuringiensis. The K+ cultures exhibit no antibacterial effect on other gram-positive and gram-negative microorganisms. The maximal level of factor K is found by the 8--9th hour of cultivation. No correlation has been established between the strucutred elements in the preparations of K+ cultures and the antibacterial activity. Apparently, factor K can be transferred to other strains.", "contents": "[Bacteriocin-like factor in Bacillus thuringiensis]. The paper describes Bacillus thuringiensis var. galleriae strains possessing a bacteriocin-like factor (factor K) which inhibits the cultures of a number of serotypes of Bac. thuringiensis. The K+ cultures exhibit no antibacterial effect on other gram-positive and gram-negative microorganisms. The maximal level of factor K is found by the 8--9th hour of cultivation. No correlation has been established between the strucutred elements in the preparations of K+ cultures and the antibacterial activity. Apparently, factor K can be transferred to other strains."}
{"id": "PMID:481278", "title": "[Proteolytic system of Aspergillus ochraceus studied in relation to its plasma-coagulating and fibrinolytic activities].", "content": "The culture of Aspergillus ochraceus HP-19 accumulates two types of proteolytic enzymes in the cultural broth. One group of proteases coagulates blood while the other has the fibrinolytic activity. The enzymes can be separated by gel filtration on Sephadex G-100. The first group of enzymes consists of several proteases with the coagulase activity and an inert protein.", "contents": "[Proteolytic system of Aspergillus ochraceus studied in relation to its plasma-coagulating and fibrinolytic activities]. The culture of Aspergillus ochraceus HP-19 accumulates two types of proteolytic enzymes in the cultural broth. One group of proteases coagulates blood while the other has the fibrinolytic activity. The enzymes can be separated by gel filtration on Sephadex G-100. The first group of enzymes consists of several proteases with the coagulase activity and an inert protein."}
{"id": "PMID:481286", "title": "[Electron microscopy study of the microorganism topography on plant roots].", "content": "The topography of microorganisms in the root cap zone and in the absorption zone was studied with 7-day-old wheat seedlings. No microorganisms were detected in the root cap zone. Individual microorganisms as well as their accumulations were registered in the absorption zone. The topography of microorganisms in this zone was characterized. Different morphological forms were present among microorganisms. Types of interaction between these microorganisms and the cells of root epidermis are discussed.", "contents": "[Electron microscopy study of the microorganism topography on plant roots]. The topography of microorganisms in the root cap zone and in the absorption zone was studied with 7-day-old wheat seedlings. No microorganisms were detected in the root cap zone. Individual microorganisms as well as their accumulations were registered in the absorption zone. The topography of microorganisms in this zone was characterized. Different morphological forms were present among microorganisms. Types of interaction between these microorganisms and the cells of root epidermis are discussed."}
{"id": "PMID:481285", "title": "[Possibility of the nonbiological binding of CO2 and CO by the components of Martian soil].", "content": "A mixture of carbon dioxide and carbon monoxide was irradiated in the presence of limonite and hydrogen peroxide at 340 nm. As has been demonstrated by gas chromatography, chemical reactions occurring in this case yield organic compounds which could be synthesized in the experiments conducted by the Viking stations. Consequently, this method cannot discriminate between the chemical and biological processes which possibly accounted for positive signals registered in the experiments of the Viking stations.", "contents": "[Possibility of the nonbiological binding of CO2 and CO by the components of Martian soil]. A mixture of carbon dioxide and carbon monoxide was irradiated in the presence of limonite and hydrogen peroxide at 340 nm. As has been demonstrated by gas chromatography, chemical reactions occurring in this case yield organic compounds which could be synthesized in the experiments conducted by the Viking stations. Consequently, this method cannot discriminate between the chemical and biological processes which possibly accounted for positive signals registered in the experiments of the Viking stations."}
{"id": "PMID:481281", "title": "[Ultrastructural organization of Sulfobacillus thermosulfidooxidans cells and spores].", "content": "The ultrastructural organization of Sulfobacillus thermosulfidooxidans cells and spores was studied by electron microscopy using the techniques of negative contrasting and thin sections. The cell wall was found to be 20--25 nm thick and to consist of a rigid layer bound to the cytoplasmic membrane with crosspieces. A microcapsule about 20 nm thick having an ordered structure is closely associated with the cell wall. A layer of slime which can reach 0.2--0.3 mcm and more in thickness is located at the outside. The flagellar apparatus is absent. The membranous apparatus has a peculiar structure. On thin cross-sections of intact cells, the cytoplasmic membrane profile is stained sharply asymmetrically. Numerous membrane invaginates located in the cytoplasm are morphologically simple vesicular formations and structures of a rather complicated unusual configuration. Lamella-like structures are also encountered, which divide, as it were, the cytoplasm into compartments. The contours of the intracellular membranes look thicker as compared with the cytoplasmic membrane. Small vesicular formations are occasionally encountered in the periplasm. Vast areas with inclusions of unknown chemical nature having a fine granular structure are located in the cytoplasm. The process of sporogenesis comprises the same stages as in Bacillus, and spores have a strucutre typical of the endospores.", "contents": "[Ultrastructural organization of Sulfobacillus thermosulfidooxidans cells and spores]. The ultrastructural organization of Sulfobacillus thermosulfidooxidans cells and spores was studied by electron microscopy using the techniques of negative contrasting and thin sections. The cell wall was found to be 20--25 nm thick and to consist of a rigid layer bound to the cytoplasmic membrane with crosspieces. A microcapsule about 20 nm thick having an ordered structure is closely associated with the cell wall. A layer of slime which can reach 0.2--0.3 mcm and more in thickness is located at the outside. The flagellar apparatus is absent. The membranous apparatus has a peculiar structure. On thin cross-sections of intact cells, the cytoplasmic membrane profile is stained sharply asymmetrically. Numerous membrane invaginates located in the cytoplasm are morphologically simple vesicular formations and structures of a rather complicated unusual configuration. Lamella-like structures are also encountered, which divide, as it were, the cytoplasm into compartments. The contours of the intracellular membranes look thicker as compared with the cytoplasmic membrane. Small vesicular formations are occasionally encountered in the periplasm. Vast areas with inclusions of unknown chemical nature having a fine granular structure are located in the cytoplasm. The process of sporogenesis comprises the same stages as in Bacillus, and spores have a strucutre typical of the endospores."}
{"id": "PMID:481288", "title": "[Microbial cenosis in the initial stage of spruce needle decomposition].", "content": "The formation of saprophytic microbial cenosis at the primary stage of decomposition of spruce needles was studied by the method of scanning electron microscopy with parallel inoculations into growth media. The composition of the cenosis was found to differ depending on whether the needles were decomposed on the surface of the forest floor or on the soil without any flooring. The characteristics of the formation of the saprophytic microbial cenosis are described. The cenosis is formed from individual species of the soil microbial complex and from some representatives of the epiphytic microbial cenosis which change here becoming saprophytes instead of biotrophs. Yeasts and yeast-like organisms predominate at the primary stage of decomposition of spruce needles.", "contents": "[Microbial cenosis in the initial stage of spruce needle decomposition]. The formation of saprophytic microbial cenosis at the primary stage of decomposition of spruce needles was studied by the method of scanning electron microscopy with parallel inoculations into growth media. The composition of the cenosis was found to differ depending on whether the needles were decomposed on the surface of the forest floor or on the soil without any flooring. The characteristics of the formation of the saprophytic microbial cenosis are described. The cenosis is formed from individual species of the soil microbial complex and from some representatives of the epiphytic microbial cenosis which change here becoming saprophytes instead of biotrophs. Yeasts and yeast-like organisms predominate at the primary stage of decomposition of spruce needles."}
{"id": "PMID:481287", "title": "[Nitrogen-fixing fungal associations with bacteria].", "content": "Nitrogen-fixing associations of fungi and bacteria have been isolated from the soil and flooring of coniferous forests. The special composition and the nitrogen-fixing activity of components in associations were determined. Pure fungal cultures did not possess the nitrogen-fixing activity. The nitrogen-fixing activity of pure bacterial cultures was always lower than that of their associatons with fungi. The possibility of existence and the specificity of species composition in the nitrogen-fixing associations of fungi and bacteria are discussed.", "contents": "[Nitrogen-fixing fungal associations with bacteria]. Nitrogen-fixing associations of fungi and bacteria have been isolated from the soil and flooring of coniferous forests. The special composition and the nitrogen-fixing activity of components in associations were determined. Pure fungal cultures did not possess the nitrogen-fixing activity. The nitrogen-fixing activity of pure bacterial cultures was always lower than that of their associatons with fungi. The possibility of existence and the specificity of species composition in the nitrogen-fixing associations of fungi and bacteria are discussed."}
{"id": "PMID:481289", "title": "[Bacterial plankton study of the shallow inlets of Possiet Bay (Sea of Japan)].", "content": "A summer-autumn maximum and a winter maximum are typical of the seasonal bacterioplanktonic developmental cycle. The minimal concentration of bacteria (biomass, 16--26 mcgC/l; production, 5 mcgC/l . day) is observed in early winter. As the water gets warmer by July, this concentration reaches its maximum (biomass, 70--75 mcgC/l; production, 70--85 mcgC/l . day). The activity of microflora in half-open bays is similar to that of the open bay the Road of Pallas. The high level of bacterial activity is evidently supported by dissolved organic matter transferred from the detrital pool of eelgrass.", "contents": "[Bacterial plankton study of the shallow inlets of Possiet Bay (Sea of Japan)]. A summer-autumn maximum and a winter maximum are typical of the seasonal bacterioplanktonic developmental cycle. The minimal concentration of bacteria (biomass, 16--26 mcgC/l; production, 5 mcgC/l . day) is observed in early winter. As the water gets warmer by July, this concentration reaches its maximum (biomass, 70--75 mcgC/l; production, 70--85 mcgC/l . day). The activity of microflora in half-open bays is similar to that of the open bay the Road of Pallas. The high level of bacterial activity is evidently supported by dissolved organic matter transferred from the detrital pool of eelgrass."}
{"id": "PMID:481297", "title": "An analysis of behavioural objectives in the teaching of anatomy from the results of marks in MCQs.", "content": "Since 1972 behavioural objectives have been presented to the students in the Department of Anatomy, University of Cape Town, for certain sections of their course but not for others. Questions set to students in certifying multiple choice examination from 1972 to 1976 were divided into two categories (a) those set on sections of the course with objectives and (b) those set on sections of the course without objectives, and analysed in terms of (i) student performance, (ii) discriminative ability of the questions. Significant differences are noted in student performance in these two categories which support the setting of student objectives. Student discrimination however is noted to be less effective in the category of questions on objectives, but not significantly so.", "contents": "An analysis of behavioural objectives in the teaching of anatomy from the results of marks in MCQs. Since 1972 behavioural objectives have been presented to the students in the Department of Anatomy, University of Cape Town, for certain sections of their course but not for others. Questions set to students in certifying multiple choice examination from 1972 to 1976 were divided into two categories (a) those set on sections of the course with objectives and (b) those set on sections of the course without objectives, and analysed in terms of (i) student performance, (ii) discriminative ability of the questions. Significant differences are noted in student performance in these two categories which support the setting of student objectives. Student discrimination however is noted to be less effective in the category of questions on objectives, but not significantly so."}
{"id": "PMID:481298", "title": "Ongoing evaluation of a behavioural sciences course for medical students: uses, methods, limitations.", "content": "This paper describes a system of ongoing student evaluation of a behavioral sciences course for medical students. Student opinion about all aspects of the course is tapped, using a variety of methods including formal and informal discussion between staff and students; and written evaluations of lectures, tutorials, textbooks, assignments, and methods of assessing students' performance. The evolution of the evaluation programme into its present form is described, together with the way in which student evaluations are used, and the limitations of these methods of evaluating a course.", "contents": "Ongoing evaluation of a behavioural sciences course for medical students: uses, methods, limitations. This paper describes a system of ongoing student evaluation of a behavioral sciences course for medical students. Student opinion about all aspects of the course is tapped, using a variety of methods including formal and informal discussion between staff and students; and written evaluations of lectures, tutorials, textbooks, assignments, and methods of assessing students' performance. The evolution of the evaluation programme into its present form is described, together with the way in which student evaluations are used, and the limitations of these methods of evaluating a course."}
{"id": "PMID:481299", "title": "A factor analytic study of branching patient management problems.", "content": "This study was undertaken to determine the factor analytic structure of patient management problems (PMPs) and to determine whether such factors are stable for different groups taking the same examination and for the same group over time. Two examinations were administered to a group of medical students, the first during their junior year and the second during their senior year. The second examination was also administered to a second class of students during their junior year. Factor analyses results indicated there are two components to medical problem-solving as measured by PMPs-data gathering and management. Both factors were stable over groups and over time.", "contents": "A factor analytic study of branching patient management problems. This study was undertaken to determine the factor analytic structure of patient management problems (PMPs) and to determine whether such factors are stable for different groups taking the same examination and for the same group over time. Two examinations were administered to a group of medical students, the first during their junior year and the second during their senior year. The second examination was also administered to a second class of students during their junior year. Factor analyses results indicated there are two components to medical problem-solving as measured by PMPs-data gathering and management. Both factors were stable over groups and over time."}
{"id": "PMID:481300", "title": "Videotapes in psychiatry: their use in teaching observation techniques.", "content": "In order to assess the effectiveness of videotapes in teaching mental state examination, two groups of medical students, one who had received videotape teaching and one not exposed to videotapes, are compared in their ability to observe accurately the mental state during a live interview. Accuracy of observation was assessed by obtaining a measure of the students' ability to shift from an incorrect opinion about a patient to a more accurate assessment following the live interview. Students who had received videotape teaching were able to make more accurate judgements than those who had not. This ability was not related to certain personality attitudes measured, nor to an ability to detect subjective elements in the interview, nor is it reflected in their examination results. The only significant variable found to influence this ability was whether or not the student had received videotape teaching.", "contents": "Videotapes in psychiatry: their use in teaching observation techniques. In order to assess the effectiveness of videotapes in teaching mental state examination, two groups of medical students, one who had received videotape teaching and one not exposed to videotapes, are compared in their ability to observe accurately the mental state during a live interview. Accuracy of observation was assessed by obtaining a measure of the students' ability to shift from an incorrect opinion about a patient to a more accurate assessment following the live interview. Students who had received videotape teaching were able to make more accurate judgements than those who had not. This ability was not related to certain personality attitudes measured, nor to an ability to detect subjective elements in the interview, nor is it reflected in their examination results. The only significant variable found to influence this ability was whether or not the student had received videotape teaching."}
{"id": "PMID:481291", "title": "[Product of second-stage riboflavin biosynthesis in Pichia guilliermondii].", "content": "Dialyzed extracts of the mutants of Pichia guilliermondii RG80 (rib3, his-) and RA39 (rib3, ade-) in which the third step of flavinogenesis was genetically blocked converted GTP into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate and 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine in the presence of NADPH2. Apparently, 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate is a true precursor of riboflavin whereas 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine is formed upon its hydrolysis.", "contents": "[Product of second-stage riboflavin biosynthesis in Pichia guilliermondii]. Dialyzed extracts of the mutants of Pichia guilliermondii RG80 (rib3, his-) and RA39 (rib3, ade-) in which the third step of flavinogenesis was genetically blocked converted GTP into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate and 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine in the presence of NADPH2. Apparently, 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate is a true precursor of riboflavin whereas 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine is formed upon its hydrolysis."}
{"id": "PMID:481301", "title": "The use of television in the assessment of home and foreign postgraduate groups during an introductory course in psychiatry.", "content": "Videotapes were developed for testing the ability to detect psychiatric phenomena, methods of questioning and interview technique. These were capable of discriminating between the performance of home graduates (HGs) and foreign graduates (FGs) and were consistent with other test results. These were significant differences between HGs and FGs on all tests before and after teaching. HGs started with high scores on all tests and these remained high, although no significant improvement occurred with teaching. FGs started with lower scores and showed improvement on the videotape test only, after teaching. HGs and FGs were exposed to video teaching methods but FGs rated video demonstration as a more helpful teaching mode.", "contents": "The use of television in the assessment of home and foreign postgraduate groups during an introductory course in psychiatry. Videotapes were developed for testing the ability to detect psychiatric phenomena, methods of questioning and interview technique. These were capable of discriminating between the performance of home graduates (HGs) and foreign graduates (FGs) and were consistent with other test results. These were significant differences between HGs and FGs on all tests before and after teaching. HGs started with high scores on all tests and these remained high, although no significant improvement occurred with teaching. FGs started with lower scores and showed improvement on the videotape test only, after teaching. HGs and FGs were exposed to video teaching methods but FGs rated video demonstration as a more helpful teaching mode."}
{"id": "PMID:481302", "title": "Criteria for acceptance of reports in experimental laboratory courses (an examination of the cook-book experiment in physiology and its relation to the scientific process).", "content": "The purpose of this paper is to solve a practical problem through a theoretical analysis based on concepts from another scientific field. A new hypothesis emerges from this 'hermeneutical' approach, namely that the criteria derived are a set of useful tools in improving instruction in laboratory course work of the Cook-Book-Experiment type in physiology and related subjects. The hypothesis has been empirically tested only to an extent that deems it worthy of further testing by other teachers.", "contents": "Criteria for acceptance of reports in experimental laboratory courses (an examination of the cook-book experiment in physiology and its relation to the scientific process). The purpose of this paper is to solve a practical problem through a theoretical analysis based on concepts from another scientific field. A new hypothesis emerges from this 'hermeneutical' approach, namely that the criteria derived are a set of useful tools in improving instruction in laboratory course work of the Cook-Book-Experiment type in physiology and related subjects. The hypothesis has been empirically tested only to an extent that deems it worthy of further testing by other teachers."}
{"id": "PMID:481306", "title": "[Evaluation of neck bruits by directional Doppler sonography (author's transl)].", "content": "Neck bruits were noticed 86 times in 60 patients. A hemodynamic obstruction of the carotid artery was diagnosed in 22% by Doppler sonography of the supratrochlear artery. The diagnostic directions in case of neck bruits are suggested: 1. In patients with a history of neurological symptoms a pathological Doppler test will be the indication for cerebral angiography. If obstruction of the carotid artery is suspected by clinical examination cerebral angiography should be done in spite of a normal directional Doppler sonography. 2. Cerebral angiography must be done in patients with asymptomatic neck bruits and a pathological Doppler sonography if the patient will undergo a great operation.", "contents": "[Evaluation of neck bruits by directional Doppler sonography (author's transl)]. Neck bruits were noticed 86 times in 60 patients. A hemodynamic obstruction of the carotid artery was diagnosed in 22% by Doppler sonography of the supratrochlear artery. The diagnostic directions in case of neck bruits are suggested: 1. In patients with a history of neurological symptoms a pathological Doppler test will be the indication for cerebral angiography. If obstruction of the carotid artery is suspected by clinical examination cerebral angiography should be done in spite of a normal directional Doppler sonography. 2. Cerebral angiography must be done in patients with asymptomatic neck bruits and a pathological Doppler sonography if the patient will undergo a great operation."}
{"id": "PMID:481307", "title": "[Survey after correction of carotid stenosis (author's transl)].", "content": "Since 1962 until 1976 we have performed 335 operations on the carotid artery in 268 patients. In 1977 we were able to obtain information from 265 patients. The early mortality in stage I, II and IV was one to three percent, in stage III 40 percent. The cumulative survival rate after four years was 74 percent. The main causes of death were cardiac diseases. Often the patients also had heart failure, peripheral arterial diseases, hypertension, and diabetes. Following the operation at least one third of the patients had an improvement of their symptoms.", "contents": "[Survey after correction of carotid stenosis (author's transl)]. Since 1962 until 1976 we have performed 335 operations on the carotid artery in 268 patients. In 1977 we were able to obtain information from 265 patients. The early mortality in stage I, II and IV was one to three percent, in stage III 40 percent. The cumulative survival rate after four years was 74 percent. The main causes of death were cardiac diseases. Often the patients also had heart failure, peripheral arterial diseases, hypertension, and diabetes. Following the operation at least one third of the patients had an improvement of their symptoms."}
{"id": "PMID:481308", "title": "[The resorption of cotrimoxazol from preparations produced by different manufacturies (author's transl)].", "content": "In a pharmacokinetic study with healthy test persons we compared the forte-tablet form of cotrimoxazol produced by 4 different manufacturers. We were able to show that 3 of the tested products (2 well known standard products I and II as well as a new comparative product) achieved nearly equal high serum concentrations of sulfamethoxazole and trimethoprim. The serum concentrations of a fourth product, a generic product, were lower for sulfamethoxazole and trimethoprim. As the difference is of no statistical significance and the serum concentrations of the last product are within the therapeutic range this product is also qualified for therapeutic use. When the dose is given after a standard breakfast the serum values of sulfamethoxazole were slightly higher than if given on an empty stomach. However, the serum values of trimethoprim given on an empty stomach were higher.", "contents": "[The resorption of cotrimoxazol from preparations produced by different manufacturies (author's transl)]. In a pharmacokinetic study with healthy test persons we compared the forte-tablet form of cotrimoxazol produced by 4 different manufacturers. We were able to show that 3 of the tested products (2 well known standard products I and II as well as a new comparative product) achieved nearly equal high serum concentrations of sulfamethoxazole and trimethoprim. The serum concentrations of a fourth product, a generic product, were lower for sulfamethoxazole and trimethoprim. As the difference is of no statistical significance and the serum concentrations of the last product are within the therapeutic range this product is also qualified for therapeutic use. When the dose is given after a standard breakfast the serum values of sulfamethoxazole were slightly higher than if given on an empty stomach. However, the serum values of trimethoprim given on an empty stomach were higher."}
{"id": "PMID:481313", "title": "[Atrial double-rhythm with total intraatrial block (author's transl)].", "content": "Report about a 52-year old patient with hypertension, who showed a transitory atrial double-rhythm and a total intraatrial block. The actions of one atrium with a frequency of 55--60/minute were transmitted regularly to the ventricles; the actions of the other atrium had a frequency of 90/minute and were not transmitted. In later controls of the ECG this phenomenon could not be seen again; at this time the rhythm was changing between a sinusrhythm with polytope ventricular extrasystoles and an absolute arrhythmia due to atrial fibrillation.", "contents": "[Atrial double-rhythm with total intraatrial block (author's transl)]. Report about a 52-year old patient with hypertension, who showed a transitory atrial double-rhythm and a total intraatrial block. The actions of one atrium with a frequency of 55--60/minute were transmitted regularly to the ventricles; the actions of the other atrium had a frequency of 90/minute and were not transmitted. In later controls of the ECG this phenomenon could not be seen again; at this time the rhythm was changing between a sinusrhythm with polytope ventricular extrasystoles and an absolute arrhythmia due to atrial fibrillation."}
{"id": "PMID:481314", "title": "[Psychological and psychopathological aspects of cardiac pacemaker implantation (author's transl)].", "content": "Several examinations made clear that patients with cardiac pacemakers are able to react in the three following ways: In most cases we see a regain of joy of living and mental performance caused by the regulation of heart-action and the normalization of the physical strength. Most patients seem to be anxious and labile in the first postoperative phase until some weeks later the previous state of emotional stability is obtained again. Many other patients are not able to adapt themselves to the pacemaker in a satisfactory manner; depressions, fears, dependences, lability, and uncomfortable feelings continue. In some cases we see intensive affective reactions even to a psychotic degree. An accurate and continous psychological care based upon an intact doctor-patient-relation might lead to an accelerated and improved adaptation to the pacemaker.", "contents": "[Psychological and psychopathological aspects of cardiac pacemaker implantation (author's transl)]. Several examinations made clear that patients with cardiac pacemakers are able to react in the three following ways: In most cases we see a regain of joy of living and mental performance caused by the regulation of heart-action and the normalization of the physical strength. Most patients seem to be anxious and labile in the first postoperative phase until some weeks later the previous state of emotional stability is obtained again. Many other patients are not able to adapt themselves to the pacemaker in a satisfactory manner; depressions, fears, dependences, lability, and uncomfortable feelings continue. In some cases we see intensive affective reactions even to a psychotic degree. An accurate and continous psychological care based upon an intact doctor-patient-relation might lead to an accelerated and improved adaptation to the pacemaker."}
{"id": "PMID:481318", "title": "Slipped capital femoral epiphysis following radiotherapy and chemotherapy.", "content": "There may be an increased frequency of slipped femoral epiphysis in pediatric patients who have received radiation to the pelvic area and combination chemotherapy. Previous reports include bilateral slipping of the epiphysis, eight years after radiation, in a patient with a pelvic neuroblastoma and the occurrence in a patient who had received radiation for Hodgkin disease, with a primary site in the pelvic region. No direct correlation was established in either case. This is a report of two patients who received pelvic radiation and combination chemotherapy who subsequently developed slipped femoral epiphysis. Our purpose is to heighten the suspicion in patients who have received radiation and chemotherapy for pelvic tumors. It is recommended that the femoral heads and acetabula be shielded when their inclusion in the treatment fields is not crucial to the treatment of the underlying disease.", "contents": "Slipped capital femoral epiphysis following radiotherapy and chemotherapy. There may be an increased frequency of slipped femoral epiphysis in pediatric patients who have received radiation to the pelvic area and combination chemotherapy. Previous reports include bilateral slipping of the epiphysis, eight years after radiation, in a patient with a pelvic neuroblastoma and the occurrence in a patient who had received radiation for Hodgkin disease, with a primary site in the pelvic region. No direct correlation was established in either case. This is a report of two patients who received pelvic radiation and combination chemotherapy who subsequently developed slipped femoral epiphysis. Our purpose is to heighten the suspicion in patients who have received radiation and chemotherapy for pelvic tumors. It is recommended that the femoral heads and acetabula be shielded when their inclusion in the treatment fields is not crucial to the treatment of the underlying disease."}
{"id": "PMID:481319", "title": "Inapparent infiltrations associated with vindesine administration.", "content": "Vindesine is des-acetyl vinblastine amide sulfate (Eldisine, Eli Lilly and Co), an investigational derivative of the vinca alkaloid, vinblastine. Initial phase I results with vindesine have determined that myelotoxicity is dose-limiting and that, contrary to preclinical studies in animals [1], a neurotoxic potential is indeed present [2]. Another phase I study also observed a rather high incidence of phlebitis (16%) and of cellulitis (10%), however, myelosuppression remained the dose-limiting toxicity [3]. Because good clinical responses have been noted in lymphomas, leukemias [4] and some solid tumors including breast cancer [5], the drug has a potential for wide utility. However, while we have noted several clinical responses with vindesine, injections of the drug have been complicated by serious infiltration with atypically delayed presentation. The purpose of this communication is to briefly describe the incidence and course of these infiltrations.", "contents": "Inapparent infiltrations associated with vindesine administration. Vindesine is des-acetyl vinblastine amide sulfate (Eldisine, Eli Lilly and Co), an investigational derivative of the vinca alkaloid, vinblastine. Initial phase I results with vindesine have determined that myelotoxicity is dose-limiting and that, contrary to preclinical studies in animals [1], a neurotoxic potential is indeed present [2]. Another phase I study also observed a rather high incidence of phlebitis (16%) and of cellulitis (10%), however, myelosuppression remained the dose-limiting toxicity [3]. Because good clinical responses have been noted in lymphomas, leukemias [4] and some solid tumors including breast cancer [5], the drug has a potential for wide utility. However, while we have noted several clinical responses with vindesine, injections of the drug have been complicated by serious infiltration with atypically delayed presentation. The purpose of this communication is to briefly describe the incidence and course of these infiltrations."}
{"id": "PMID:481320", "title": "Action of cytosine arabinoside on human tumor cells isolated from malignant effusions: in vitro phosphorylation and inhibition of DNA synthesis.", "content": "Tumor cells were isolated from malignant effusions of three patients with disseminated solid tumors of different origin. Intracellular accumulation of nondiffusible cytosine arabinoside (ara-C) nucleotides was used to measure phosphorylation. Mouse leukemia L 1210 and L 1210/CA, and ara-C-resistant subline, were used as reference cells. Phosphorylation activity was similar in the cells from all three solid tumors and showed a linear increase with drug concentrations of 0.1--100 micograms/ml. In contrast to activity in L 1210 cells, the in vitro activity was not saturable at drug levels up to 100 micrograms/ml. Ara-C inhibited the incorporation of thymidine into DNA 84%--90% in the solid tumor cells at a concentration of 10 micrograms/ml. Higher drug concentrations did not result in further inhibition. In one patient, DNA synthesis of tumor cells isolated before and after intraperitoneal instillation of 1,000 mg ara-C was measured. The in vivo inhibition was found to correspond to the in vitro data. Solid tumor cells isolated from malignant effusion have no greatly reduced capacity for cellular formation of ara-C/nucleotides, but higher drug levels than achieved with conventional therapy are necessary for sufficient ara-C nucleotide synthesis.", "contents": "Action of cytosine arabinoside on human tumor cells isolated from malignant effusions: in vitro phosphorylation and inhibition of DNA synthesis. Tumor cells were isolated from malignant effusions of three patients with disseminated solid tumors of different origin. Intracellular accumulation of nondiffusible cytosine arabinoside (ara-C) nucleotides was used to measure phosphorylation. Mouse leukemia L 1210 and L 1210/CA, and ara-C-resistant subline, were used as reference cells. Phosphorylation activity was similar in the cells from all three solid tumors and showed a linear increase with drug concentrations of 0.1--100 micograms/ml. In contrast to activity in L 1210 cells, the in vitro activity was not saturable at drug levels up to 100 micrograms/ml. Ara-C inhibited the incorporation of thymidine into DNA 84%--90% in the solid tumor cells at a concentration of 10 micrograms/ml. Higher drug concentrations did not result in further inhibition. In one patient, DNA synthesis of tumor cells isolated before and after intraperitoneal instillation of 1,000 mg ara-C was measured. The in vivo inhibition was found to correspond to the in vitro data. Solid tumor cells isolated from malignant effusion have no greatly reduced capacity for cellular formation of ara-C/nucleotides, but higher drug levels than achieved with conventional therapy are necessary for sufficient ara-C nucleotide synthesis."}
{"id": "PMID:481321", "title": "An exploration of cancer staff attitudes and values.", "content": "A multidisciplinary team involved in the care of patients with advanced cancer including physicians, nurses, social workers, physical therapists, nutritionists, clergymen, enterostomal therapists, biostatisticians and administrative personnel were studied over a period of eighteen months. A prospective study of staff attitudes and values about life, disease, and medical care which focused particularly on cancer, cancer treatment and patients with cancer was undertaken. Data were gathered from the staff by written responses to the following instruments: 1) Rokeach Value Survey and 2) a Personal Attitudes Toward Illness Questionnaire. This longitudinal prospective study demonstrated the reliability of scrutinizing individual and collective staff characteristics in a cancer care team. Although most team members had minimal previous contact with clinical research or with patients with advanced cancer, increased exposure did not result in changes in staff attitudes and values over the period of seventeen months.", "contents": "An exploration of cancer staff attitudes and values. A multidisciplinary team involved in the care of patients with advanced cancer including physicians, nurses, social workers, physical therapists, nutritionists, clergymen, enterostomal therapists, biostatisticians and administrative personnel were studied over a period of eighteen months. A prospective study of staff attitudes and values about life, disease, and medical care which focused particularly on cancer, cancer treatment and patients with cancer was undertaken. Data were gathered from the staff by written responses to the following instruments: 1) Rokeach Value Survey and 2) a Personal Attitudes Toward Illness Questionnaire. This longitudinal prospective study demonstrated the reliability of scrutinizing individual and collective staff characteristics in a cancer care team. Although most team members had minimal previous contact with clinical research or with patients with advanced cancer, increased exposure did not result in changes in staff attitudes and values over the period of seventeen months."}
{"id": "PMID:481322", "title": "Adenocarcinoma of unknown primary site: a clinico-pathological study.", "content": "An analysis of the clinico-pathological findings in 49 patients with adenocarcinoma of unknown primary origin indicates a short survival time. No factors were identified that influenced survival. Thromboembolic episodes and second primary tumors commonly complicate the clinical course. Metastases in widespread organs are common autopsy findings. Immunological mechanisms may explain these findings.", "contents": "Adenocarcinoma of unknown primary site: a clinico-pathological study. An analysis of the clinico-pathological findings in 49 patients with adenocarcinoma of unknown primary origin indicates a short survival time. No factors were identified that influenced survival. Thromboembolic episodes and second primary tumors commonly complicate the clinical course. Metastases in widespread organs are common autopsy findings. Immunological mechanisms may explain these findings."}
{"id": "PMID:481353", "title": "A megavoltage MTF measurement technique for metal screen-film detectors.", "content": "A technique to measure the MTF of metal screen-film detectors at megavoltage x-ray energies has been devised. It employs an exposure through a slit 25 micron wide and a method to average numerous line spread functions from a single film image. That the slit is wide compared to those used at diagnostic x-ray energies serves two purposes: it facilitates the use of a laser alignment procedure designed to ensure correct orientation of the slit-forming tungsten blocks in the x-ray beam, and it also increases the contrast in the slit image, thereby reducing the data analysis problems caused by film noise. Further reduction in the effects of film noise is accomplished by the averaging method. A correction is made to account for the effect of the wide slit on the MTF. The validity of this correction, the laser alignment, and the averaging method have been confirmed by simulations.", "contents": "A megavoltage MTF measurement technique for metal screen-film detectors. A technique to measure the MTF of metal screen-film detectors at megavoltage x-ray energies has been devised. It employs an exposure through a slit 25 micron wide and a method to average numerous line spread functions from a single film image. That the slit is wide compared to those used at diagnostic x-ray energies serves two purposes: it facilitates the use of a laser alignment procedure designed to ensure correct orientation of the slit-forming tungsten blocks in the x-ray beam, and it also increases the contrast in the slit image, thereby reducing the data analysis problems caused by film noise. Further reduction in the effects of film noise is accomplished by the averaging method. A correction is made to account for the effect of the wide slit on the MTF. The validity of this correction, the laser alignment, and the averaging method have been confirmed by simulations."}
{"id": "PMID:481354", "title": "Examination of the factors Ac and Aeq for cylindrical ion chambers used in cobalt-60 beams.", "content": "The calibration of a cobalt-60 beam in a phantom with an ion chamber, which has been calibrated with respect to exposure, requires the use of a displacement correction factor which essentially corrects the photon fluence for the attenuation and scatter when the chamber with buildup cap is removed and replaced by phantom material. To determine the displacement factor, Ac, a special set of cylindrical ionization chambers with various volumes were constructed out of polystyrene. Tissue-air ratios were measured with these chambers for cobalt-60 gamma rays in a polystyrene phantom, and the ratio Ac/Aeq was experimentally determined. In order to calculate Ac from this ratio, Aeq was determined also. It was found that Ac depended on chamber diameter only, and not on field size or depth. A value of 0.990 for Aeq is recommended and a table of Ac for chambers of different outer diameters is included.", "contents": "Examination of the factors Ac and Aeq for cylindrical ion chambers used in cobalt-60 beams. The calibration of a cobalt-60 beam in a phantom with an ion chamber, which has been calibrated with respect to exposure, requires the use of a displacement correction factor which essentially corrects the photon fluence for the attenuation and scatter when the chamber with buildup cap is removed and replaced by phantom material. To determine the displacement factor, Ac, a special set of cylindrical ionization chambers with various volumes were constructed out of polystyrene. Tissue-air ratios were measured with these chambers for cobalt-60 gamma rays in a polystyrene phantom, and the ratio Ac/Aeq was experimentally determined. In order to calculate Ac from this ratio, Aeq was determined also. It was found that Ac depended on chamber diameter only, and not on field size or depth. A value of 0.990 for Aeq is recommended and a table of Ac for chambers of different outer diameters is included."}
{"id": "PMID:481360", "title": "The laser microprobe mass analyzer (LAMMA): a new instrument for biomedical microprobe analysis.", "content": "After a short review of the general state of the art of microprobe analysis, recent achievements of a new system, the laser microprobe mass analyzer (LAMMA) are presented. The LAMMA-instrument, which was primarily developed for biomedical purposes, has recently been used on a large variety of specimen, biological, technical, organic and inorganic in nature. Detection limits, mass resolution and reproducibility were improved such that for many elements measurements could be extended down to the subppm range. For bulk analysis, a separate version of the LAMMA-instrument is under development. The examples of LAMMA-applications given in this paper include muscle tissue (physiological cations), uterine tissue (Fe), retina tissues (Na, Mgmg, K, Ca and Ba!), airborne particles (Pb, Fe, Al, Ti, Mn, Ca, Zn), cerebral tissue (Li), and pure cristalline organic materials.", "contents": "The laser microprobe mass analyzer (LAMMA): a new instrument for biomedical microprobe analysis. After a short review of the general state of the art of microprobe analysis, recent achievements of a new system, the laser microprobe mass analyzer (LAMMA) are presented. The LAMMA-instrument, which was primarily developed for biomedical purposes, has recently been used on a large variety of specimen, biological, technical, organic and inorganic in nature. Detection limits, mass resolution and reproducibility were improved such that for many elements measurements could be extended down to the subppm range. For bulk analysis, a separate version of the LAMMA-instrument is under development. The examples of LAMMA-applications given in this paper include muscle tissue (physiological cations), uterine tissue (Fe), retina tissues (Na, Mgmg, K, Ca and Ba!), airborne particles (Pb, Fe, Al, Ti, Mn, Ca, Zn), cerebral tissue (Li), and pure cristalline organic materials."}
{"id": "PMID:481355", "title": "Dose distribution from a Delta-25 head scanner.", "content": "The dose distribution pattern from a Delta-25 head scanner was evaluated experimentally in a 17.5-cm-diam cylinder water phantom for different scan speed and slice thickness combinations. TLD chips were used to measure the dose at different points across the scan section. The isodose curves thus obtained were found to be asymmetric, with the regions of maximum and minimum dose near the surface on the opposite sides of the phantom. The effect of changing the scan thickness and speed on dose was evaluated and a linear increase in dose with the increase of slice thickness was observed. This was consistent with the design of the line focus and the beam collimators used in this system. The effect of multiple slice scan on the dose was also evaluated.", "contents": "Dose distribution from a Delta-25 head scanner. The dose distribution pattern from a Delta-25 head scanner was evaluated experimentally in a 17.5-cm-diam cylinder water phantom for different scan speed and slice thickness combinations. TLD chips were used to measure the dose at different points across the scan section. The isodose curves thus obtained were found to be asymmetric, with the regions of maximum and minimum dose near the surface on the opposite sides of the phantom. The effect of changing the scan thickness and speed on dose was evaluated and a linear increase in dose with the increase of slice thickness was observed. This was consistent with the design of the line focus and the beam collimators used in this system. The effect of multiple slice scan on the dose was also evaluated."}
{"id": "PMID:481361", "title": "Fast and economic conversion of images to a tactile presentation for blind people.", "content": "A new procedure for the conversion of binary black and white images to a tactile presentation perceptible by the blind is described. Conventional procedures are very time-consuming and expensive and are not adapted to produce the required low editions of tactile displays, e.g., for schools for the blind or individual applications. The procedure recently developed allows the fast, economical conversion of even single drawings to a tactile display with high resolution. An account of the investigations and valuations of all possible physical procedures of optical-to-tactile conversion is given. The new procedure, based on a slightly modified Xerox copying process in a first step and gumming tactile material with the tone powder at the darkened regions in a second step, is described in detail. The device realised is presented. It allows the conversion of one page of a drawing to a tactile display with a resolution of 0.3 mm within 1 min. Various applications of the system and experiences with blind users are reported. The implement proved to meet most of the requirements of blind people. It is easy to handle and produces tactile drawings of excellent quality. As it is based on commercial subsystems it promises an economical production even at small series.", "contents": "Fast and economic conversion of images to a tactile presentation for blind people. A new procedure for the conversion of binary black and white images to a tactile presentation perceptible by the blind is described. Conventional procedures are very time-consuming and expensive and are not adapted to produce the required low editions of tactile displays, e.g., for schools for the blind or individual applications. The procedure recently developed allows the fast, economical conversion of even single drawings to a tactile display with high resolution. An account of the investigations and valuations of all possible physical procedures of optical-to-tactile conversion is given. The new procedure, based on a slightly modified Xerox copying process in a first step and gumming tactile material with the tone powder at the darkened regions in a second step, is described in detail. The device realised is presented. It allows the conversion of one page of a drawing to a tactile display with a resolution of 0.3 mm within 1 min. Various applications of the system and experiences with blind users are reported. The implement proved to meet most of the requirements of blind people. It is easy to handle and produces tactile drawings of excellent quality. As it is based on commercial subsystems it promises an economical production even at small series."}
{"id": "PMID:481356", "title": "Monte Carlo advice.", "content": "The generation of long, high quality random number sequences for Monte Carlo simulations using minicomputers is considered. The importance of the thorough testing of Monte Carlo random number generators is emphasized. A recommendation is given to authors of Monte Carlo papers to specify their random number generator and to describe the randomness testing which that generator has undergone.", "contents": "Monte Carlo advice. The generation of long, high quality random number sequences for Monte Carlo simulations using minicomputers is considered. The importance of the thorough testing of Monte Carlo random number generators is emphasized. A recommendation is given to authors of Monte Carlo papers to specify their random number generator and to describe the randomness testing which that generator has undergone."}
{"id": "PMID:481362", "title": "Discrimination of phantom hand sensations elicited by afferent electrical nerve stimulation in below-elbow amputees.", "content": "The necessity for a sensory feedback system that would enhance patient acceptability of motorized hand prostheses is now generally acknowledged. Afferent electrical stimulation of the nerves in the amputation stump can convey sensory feedback from prostheses with the advantage of eliciting sensations in the phantom image of the lost hand. Experiments with percutaneous nerve stimulation of the amputation stump in below-elbow amputees showed that with stable electrode conditions, amplitude modulated stimulation was better than frequency modulated stimulation in terms of accuracy, delay, and transinformation both with intermittent and uninterrupted stimulation. With unstable electrode conditions, different results were noticed, since amplitude modulated stimulation is very sensitive even to minor changes in electrode position. It is concluded that afferent electrical nerve stimulation with adequate training and stable electrodes had characteristics of accuracy, transinformation and delay which are good enough to make it a suitable method of conveying information in a prosthesis feedback system.", "contents": "Discrimination of phantom hand sensations elicited by afferent electrical nerve stimulation in below-elbow amputees. The necessity for a sensory feedback system that would enhance patient acceptability of motorized hand prostheses is now generally acknowledged. Afferent electrical stimulation of the nerves in the amputation stump can convey sensory feedback from prostheses with the advantage of eliciting sensations in the phantom image of the lost hand. Experiments with percutaneous nerve stimulation of the amputation stump in below-elbow amputees showed that with stable electrode conditions, amplitude modulated stimulation was better than frequency modulated stimulation in terms of accuracy, delay, and transinformation both with intermittent and uninterrupted stimulation. With unstable electrode conditions, different results were noticed, since amplitude modulated stimulation is very sensitive even to minor changes in electrode position. It is concluded that afferent electrical nerve stimulation with adequate training and stable electrodes had characteristics of accuracy, transinformation and delay which are good enough to make it a suitable method of conveying information in a prosthesis feedback system."}
{"id": "PMID:481357", "title": "Calculating dose from remaining body activity: a comparison of two methods.", "content": "Two methods for calculating the radiation dose from remaining body activity have been suggested. One requires correction of the cumulated activities so that they reflect the activity uniformly distributed in the total body. The other method requires correction of the S values so that a value of S for the target organ from the remainder of the body is obtained. These two methods give the same answer. We have examined these methods and the number of steps required to calculate the radiation dose in each case. Our results show that the method of correcting the cumulated activities is preferred, especially if the number of source and target organs is large and a computer equipped with the necessary software is not available.", "contents": "Calculating dose from remaining body activity: a comparison of two methods. Two methods for calculating the radiation dose from remaining body activity have been suggested. One requires correction of the cumulated activities so that they reflect the activity uniformly distributed in the total body. The other method requires correction of the S values so that a value of S for the target organ from the remainder of the body is obtained. These two methods give the same answer. We have examined these methods and the number of steps required to calculate the radiation dose in each case. Our results show that the method of correcting the cumulated activities is preferred, especially if the number of source and target organs is large and a computer equipped with the necessary software is not available."}
{"id": "PMID:481363", "title": "The development of consensus standards for medical devices in Canada.", "content": "The Canadian Standards Association Health Care Technology Program is a new, innovative program initiated by the Canadian Standards Association four years ago in response to the great increase in technology which has innundated our health care facilities. The goal of the program is to develop consensus standards in the medical engineering field and to help in implementing those standards across Canada through education, input to professional societies, and input to provincial or federal legislation.", "contents": "The development of consensus standards for medical devices in Canada. The Canadian Standards Association Health Care Technology Program is a new, innovative program initiated by the Canadian Standards Association four years ago in response to the great increase in technology which has innundated our health care facilities. The goal of the program is to develop consensus standards in the medical engineering field and to help in implementing those standards across Canada through education, input to professional societies, and input to provincial or federal legislation."}
{"id": "PMID:481364", "title": "A video-recording system for evaluating the clinical interview--a simple, inexpensive method.", "content": "A simple, inexpensive video-recording system was designed to provide raw data for the evaluation of therapist interview behaviors in the out-patient clinic. The system uses a combination of commercially available cameras, lenses, and recording equipment which do not require technical audio-video expertise to operate. Video-cassette recordings are produced that contain full-face, head-and-shoulders images of both the therapist and the patient. An automatic search control terminal rapidly locates any point on the video-cassette for review. Using normal clinic lighting and little space, the system is easily installed in any existing clinic examination room. A description of the system's use in examining the counseling and interviewing skills of a medical clinic staff is presented.", "contents": "A video-recording system for evaluating the clinical interview--a simple, inexpensive method. A simple, inexpensive video-recording system was designed to provide raw data for the evaluation of therapist interview behaviors in the out-patient clinic. The system uses a combination of commercially available cameras, lenses, and recording equipment which do not require technical audio-video expertise to operate. Video-cassette recordings are produced that contain full-face, head-and-shoulders images of both the therapist and the patient. An automatic search control terminal rapidly locates any point on the video-cassette for review. Using normal clinic lighting and little space, the system is easily installed in any existing clinic examination room. A description of the system's use in examining the counseling and interviewing skills of a medical clinic staff is presented."}
{"id": "PMID:481358", "title": "Computerized patient contours using the scanning arm of compound B-scanner.", "content": "Full utilization of the precision of newer radiation therapy devices requires patient contours drawn with greater accuracy than is possible with the conventional lead wire technique. Polaroid photographs can introduce large errors due to distortion and small image size. Techniques including electromechanical or optical devices and CT scans offer improved accuracy, but often at added expense. A method for obtaining contours has been developed which utilizes a treatment planning minicomputer (equipped with an analog-to-digital converter and plotter) and a commercially available ultrasound B-scanning arm. Voltages corresponding to the X-Y position of the tip of the scanning arm are fed from the scanner to the A/D interface, smoothed, scaled, and plotted. The resulting drawing is a full scale external patient contour. The accuracy of this method is compared to alternative techniques.", "contents": "Computerized patient contours using the scanning arm of compound B-scanner. Full utilization of the precision of newer radiation therapy devices requires patient contours drawn with greater accuracy than is possible with the conventional lead wire technique. Polaroid photographs can introduce large errors due to distortion and small image size. Techniques including electromechanical or optical devices and CT scans offer improved accuracy, but often at added expense. A method for obtaining contours has been developed which utilizes a treatment planning minicomputer (equipped with an analog-to-digital converter and plotter) and a commercially available ultrasound B-scanning arm. Voltages corresponding to the X-Y position of the tip of the scanning arm are fed from the scanner to the A/D interface, smoothed, scaled, and plotted. The resulting drawing is a full scale external patient contour. The accuracy of this method is compared to alternative techniques."}
{"id": "PMID:481365", "title": "Feedback control dynamics for glucose controlled insulin infusion system.", "content": "Miles Laboratories has developed a Glucose Controlled Insulin Infusion System (GCIIS) designated by the Trademark (BIOSTATOR) as a tool to investigate the physiologic control parameters of carbohydrate metabolism and regulatory deficiencies in diabetes. It consists, in principle, of a rapid on-line glucose analyzer, a computer/controller for the calculation and control of insulin or dextrose infusion, and a multichannel infusion system. A silent printer records, on a minute-by-minute basis, the glucose value measured, the insulin and/or dextrose infusion rates, and the cumulative total of the insulin infused. The on-line glucose analyzer employs an electrochemical sensor with immobilized glucose oxidase and measures the hydrogen peroxide produced. Its linearity extends beyond 700 mg/dl glucose; it permits a rapid two-point calibration of the sensor and the overall calibration of the on-line analyzer without disconnecting the catheter from the patient. The system's response time, including blood sample transport from the patient, is less than 90 seconds with a blood loss of approximately 50 ml per 24 h. The insulin and dextrose infusions are governed by control algorithms; various mathematical models have been developed and employed toward improved feedback control dynamics. The rapid glucose analyzer eliminates the need for the calculation of a \"predicted\" glucose value and permits, instead, the use of a derivative function for dynamic control. A multichannel infusion system combines the principles of a peristaltic pump with the advantages of a precision pump performance and individual computer control for each of the pump channels.", "contents": "Feedback control dynamics for glucose controlled insulin infusion system. Miles Laboratories has developed a Glucose Controlled Insulin Infusion System (GCIIS) designated by the Trademark (BIOSTATOR) as a tool to investigate the physiologic control parameters of carbohydrate metabolism and regulatory deficiencies in diabetes. It consists, in principle, of a rapid on-line glucose analyzer, a computer/controller for the calculation and control of insulin or dextrose infusion, and a multichannel infusion system. A silent printer records, on a minute-by-minute basis, the glucose value measured, the insulin and/or dextrose infusion rates, and the cumulative total of the insulin infused. The on-line glucose analyzer employs an electrochemical sensor with immobilized glucose oxidase and measures the hydrogen peroxide produced. Its linearity extends beyond 700 mg/dl glucose; it permits a rapid two-point calibration of the sensor and the overall calibration of the on-line analyzer without disconnecting the catheter from the patient. The system's response time, including blood sample transport from the patient, is less than 90 seconds with a blood loss of approximately 50 ml per 24 h. The insulin and dextrose infusions are governed by control algorithms; various mathematical models have been developed and employed toward improved feedback control dynamics. The rapid glucose analyzer eliminates the need for the calculation of a \"predicted\" glucose value and permits, instead, the use of a derivative function for dynamic control. A multichannel infusion system combines the principles of a peristaltic pump with the advantages of a precision pump performance and individual computer control for each of the pump channels."}
{"id": "PMID:481366", "title": "The development of an artificial beta cell system and its validation in depancreatized dogs: the physiological restoration of blood glucose homeostasis.", "content": "An instrument for the control of blood glucose in a closed-loop fashion has been developed. This artificial beta cell consists of 4 subunits: a Glucose Monitor, a Microcomputer, an Insulin Infusion Pump and a Printer. Its internal control algorithm was derived from the modeled dynamics of glucose-induced insulin secretion, and includes the effects of both the proportional and derivative actions of blood glucose concentration, and is expressed as: IIR = Kp . BG + Kd . deltaBG + Kc, where IIR is insulin infusion rate, BG and deltaBG are blood glucose concentration and rate of change in glycemia, respectively. Kp and Kd are individual coefficients and Kc is a constant of basal insulin secretion. The performance of this system was studied with various kinds of glucose challenges on anaesthetized depancreatized dogs. Its notable characteristics were: 1) the insulin infusion rate was such as to maintain physiological insulin levels, and 2) dextrose or glucagon infusions were not required.", "contents": "The development of an artificial beta cell system and its validation in depancreatized dogs: the physiological restoration of blood glucose homeostasis. An instrument for the control of blood glucose in a closed-loop fashion has been developed. This artificial beta cell consists of 4 subunits: a Glucose Monitor, a Microcomputer, an Insulin Infusion Pump and a Printer. Its internal control algorithm was derived from the modeled dynamics of glucose-induced insulin secretion, and includes the effects of both the proportional and derivative actions of blood glucose concentration, and is expressed as: IIR = Kp . BG + Kd . deltaBG + Kc, where IIR is insulin infusion rate, BG and deltaBG are blood glucose concentration and rate of change in glycemia, respectively. Kp and Kd are individual coefficients and Kc is a constant of basal insulin secretion. The performance of this system was studied with various kinds of glucose challenges on anaesthetized depancreatized dogs. Its notable characteristics were: 1) the insulin infusion rate was such as to maintain physiological insulin levels, and 2) dextrose or glucagon infusions were not required."}
{"id": "PMID:481427", "title": "Action of staphylococcal alpha-toxin on membranes: some recent advances.", "content": "Recent developments in the area of Staphylococcal alpha-toxin studies are presented which modify the concepts previously held with respect to both biological and physical properties of alpha-toxin. New data concerning the nature of the binding site for alpha-toxin on rabbit erythrocyte membranes and a model to explain the various observed complexes of alpha-toxin and membrane receptor are discussed. Finally, evidence suggesting that Staphylococcal alpha-toxin is a potent demyelinating agent is presented.", "contents": "Action of staphylococcal alpha-toxin on membranes: some recent advances. Recent developments in the area of Staphylococcal alpha-toxin studies are presented which modify the concepts previously held with respect to both biological and physical properties of alpha-toxin. New data concerning the nature of the binding site for alpha-toxin on rabbit erythrocyte membranes and a model to explain the various observed complexes of alpha-toxin and membrane receptor are discussed. Finally, evidence suggesting that Staphylococcal alpha-toxin is a potent demyelinating agent is presented."}
{"id": "PMID:481428", "title": "Purification and structural properties of isozymes of isocitrate dehydrogenase from the mouse.", "content": "Cytoplasmic and mitochondrial isozymes of NADP+-dependent isocitrate dehydrogenase were purified from kidney and heart tissue of an inbred strain of mice. The cytoplasmic isozyme was purified from kidney of DBA/2J mice by means of a four-step procedure which included affinity chromatography with an 8-(6-aminohexyl)-amino-NADP+-Sepharose column. The heart mitochondrial isozyme of DBA/2J mice was purified by a two-step procedure involving the use of 8-(6-aminohexyl)-amino-AMP-Sepharose and 8-(6-aminohexyl)-amino-NADP+-Sepharose columns. The specific activity of the homogeneous cytoplasmic and mitochondrial isozymes was 40 units/mg and 45 units/mg, respectively. Native and subunit molecular weights of these two isozymes were determined by chromatography on Sephadex G-100, G-150 and G-200 Superfine and polyacrylamide gel electrophoresis. Both isozymes were found to be dimers with the subunit molecular weight of approximately 35,000. The sedimentation coefficients were determined to be 5.9 and 6.1 for the mitochondrial and cytoplasmic isozyme, respectively. The amino acid compositions of these two isozymes revealed distinct differences in arginine and proline contents. A modified procedure regarding the use of affinity columns for the purification of the weakly bound enzymes is also discussed.", "contents": "Purification and structural properties of isozymes of isocitrate dehydrogenase from the mouse. Cytoplasmic and mitochondrial isozymes of NADP+-dependent isocitrate dehydrogenase were purified from kidney and heart tissue of an inbred strain of mice. The cytoplasmic isozyme was purified from kidney of DBA/2J mice by means of a four-step procedure which included affinity chromatography with an 8-(6-aminohexyl)-amino-NADP+-Sepharose column. The heart mitochondrial isozyme of DBA/2J mice was purified by a two-step procedure involving the use of 8-(6-aminohexyl)-amino-AMP-Sepharose and 8-(6-aminohexyl)-amino-NADP+-Sepharose columns. The specific activity of the homogeneous cytoplasmic and mitochondrial isozymes was 40 units/mg and 45 units/mg, respectively. Native and subunit molecular weights of these two isozymes were determined by chromatography on Sephadex G-100, G-150 and G-200 Superfine and polyacrylamide gel electrophoresis. Both isozymes were found to be dimers with the subunit molecular weight of approximately 35,000. The sedimentation coefficients were determined to be 5.9 and 6.1 for the mitochondrial and cytoplasmic isozyme, respectively. The amino acid compositions of these two isozymes revealed distinct differences in arginine and proline contents. A modified procedure regarding the use of affinity columns for the purification of the weakly bound enzymes is also discussed."}
{"id": "PMID:481429", "title": "Mutants of Staphylococcus aureus deficient in recombinational repair. Improved isolation by selecting for mutants exhibiting concurrent sensitivity to ultraviolet radiation and N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "Recombination-deficient (rec) mutants of Staphylococcus aureus strains 152 and Ps29 were sought by initially screening mutagenized cultures for mutants exhibiting increased sensitivity to both ultraviolet (UV) radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NG). Mutants thus isolated were analyzed for recombinational ability by transduction, and further characterized in terms of sensitivity to UV, NG, ability to repair UV-irradiated bacteriophage, and spontaneous and UV-induced DNA degradation. Mutagenesis of strain 152 yielded three isolates, one of which was rec, the second potentially lex, and the third possessing an undetermined repair deficiency. Mutagenesis of strain Ps29 resulted in the isolation of one mutant, which exhibited a rec genotype. In searching for rec mutants of S. aureus, the value of initially screening mutagenized cultures for mutants exhibiting concurrent sensitivity to UV and NG, as opposed to screening for UV sensitivity alone, is discussed.", "contents": "Mutants of Staphylococcus aureus deficient in recombinational repair. Improved isolation by selecting for mutants exhibiting concurrent sensitivity to ultraviolet radiation and N-methyl-N'-nitro-N-nitrosoguanidine. Recombination-deficient (rec) mutants of Staphylococcus aureus strains 152 and Ps29 were sought by initially screening mutagenized cultures for mutants exhibiting increased sensitivity to both ultraviolet (UV) radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NG). Mutants thus isolated were analyzed for recombinational ability by transduction, and further characterized in terms of sensitivity to UV, NG, ability to repair UV-irradiated bacteriophage, and spontaneous and UV-induced DNA degradation. Mutagenesis of strain 152 yielded three isolates, one of which was rec, the second potentially lex, and the third possessing an undetermined repair deficiency. Mutagenesis of strain Ps29 resulted in the isolation of one mutant, which exhibited a rec genotype. In searching for rec mutants of S. aureus, the value of initially screening mutagenized cultures for mutants exhibiting concurrent sensitivity to UV and NG, as opposed to screening for UV sensitivity alone, is discussed."}
{"id": "PMID:481430", "title": "Vitamin C is positive in the DNA synthesis inhibition and sister-chromatid exchange tests.", "content": "Ascorbate caused a dose-dependent increase in sister-chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells and in human lymphocytes. Moreover, in the DNA synthesis inhibition test with HeLa cells, ascorbate gave results typical of DNA-damaging chemicals. Catalase reduced SCE induction by ascorbate, prevented its cytotoxicity in CHO cells, and prevented its effect on HeLa DNA synthesis. Ascorbate reduced induction of SCE in CHO cells by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) by direct inactivation of MNNG.", "contents": "Vitamin C is positive in the DNA synthesis inhibition and sister-chromatid exchange tests. Ascorbate caused a dose-dependent increase in sister-chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells and in human lymphocytes. Moreover, in the DNA synthesis inhibition test with HeLa cells, ascorbate gave results typical of DNA-damaging chemicals. Catalase reduced SCE induction by ascorbate, prevented its cytotoxicity in CHO cells, and prevented its effect on HeLa DNA synthesis. Ascorbate reduced induction of SCE in CHO cells by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) by direct inactivation of MNNG."}
{"id": "PMID:481431", "title": "Spontaneous and mitomycin-C induced sister-chromatid exchanges. Comparison of in vivo and in vitro systems.", "content": "Frequencies of sister-chromatid exchanges (SCE) were measured in vitro in mouse fibroblasts and in vivo in mouse bone-marrow cells. SCE levels in these cell systems were measured in response to varying concentrations of bromode-oxyuridine (BrdU) and mitomycin-C (MMC). Although BrdU was found to induce SCE in both cellular systems, baseline SCE levels were 2- to 3-fold higher in vitro than in vivo. SCE induction was found to be a linear function of MMC concentration in vivo and in vitro; however the slope of the vivo curve was 5-fold higher. The interaction of BrdU substituted DNA and MMC was examined by administering a fixed dose of MMC with increasing concentrations of BrdU. The induced SCE frequencies appeared to be additive. In addition to measuring drug-induced SCE, the BrdU differential staining technique allows concomitant measurement of the inhibition of cellular replication by the test drugs.", "contents": "Spontaneous and mitomycin-C induced sister-chromatid exchanges. Comparison of in vivo and in vitro systems. Frequencies of sister-chromatid exchanges (SCE) were measured in vitro in mouse fibroblasts and in vivo in mouse bone-marrow cells. SCE levels in these cell systems were measured in response to varying concentrations of bromode-oxyuridine (BrdU) and mitomycin-C (MMC). Although BrdU was found to induce SCE in both cellular systems, baseline SCE levels were 2- to 3-fold higher in vitro than in vivo. SCE induction was found to be a linear function of MMC concentration in vivo and in vitro; however the slope of the vivo curve was 5-fold higher. The interaction of BrdU substituted DNA and MMC was examined by administering a fixed dose of MMC with increasing concentrations of BrdU. The induced SCE frequencies appeared to be additive. In addition to measuring drug-induced SCE, the BrdU differential staining technique allows concomitant measurement of the inhibition of cellular replication by the test drugs."}
{"id": "PMID:481432", "title": "Frequency changes with time in vivo of radiation-induced chromosomal aberrations in skin fibroblasts.", "content": "5 Syrian hamster litter mates were each irradiated with X-rays on one flank to 300 rad. Skin biopsies were taken from both the irradiated and unirradiated (control) flanks of each animal at one day and at about 6 months after irradiation. The cells cultured from these biopsies were used to determine the frequencies of chromosomal aberrations. During the 6-month period there were significant reductions in the frequencies of both reciprocal translocations and terminal deletions. Translocations involving the short arm of the Y-chromosome, however, showed a significant increase during this period. It is possible that while the latter phenomenon was due to cell selection in vivo the general fall off in translocations and deletions was the result of a long term in vivo repair mechanism or perhaps the results of certain aberrations proving to be lethal with prolonged expression times.", "contents": "Frequency changes with time in vivo of radiation-induced chromosomal aberrations in skin fibroblasts. 5 Syrian hamster litter mates were each irradiated with X-rays on one flank to 300 rad. Skin biopsies were taken from both the irradiated and unirradiated (control) flanks of each animal at one day and at about 6 months after irradiation. The cells cultured from these biopsies were used to determine the frequencies of chromosomal aberrations. During the 6-month period there were significant reductions in the frequencies of both reciprocal translocations and terminal deletions. Translocations involving the short arm of the Y-chromosome, however, showed a significant increase during this period. It is possible that while the latter phenomenon was due to cell selection in vivo the general fall off in translocations and deletions was the result of a long term in vivo repair mechanism or perhaps the results of certain aberrations proving to be lethal with prolonged expression times."}
{"id": "PMID:481433", "title": "Mutagenicity of 8-methoxypsoralen and long-wave ultraviolet irradiation in V-79 Chinese hamster cells. A first approach to a risk estimate in photochemotherapy.", "content": "The effect of 8-methoxypsoralen (8-MOP) and long-wave ultraviolet irradiation (UVA) on cell killing and mutation induction was studied in V-79 Chinese hamster cells. No effect was observed after treatment with 8-MOP alone (50 microgram/ml, 4 h), UVA alone (9000 J/m2), or 8-MOP metabolized by rat-liver microsomes. Combined treatment with 8-MOP and UVA induced both cell killing and mutation. This was also observed under conditions approaching patient treatment with PUVA photochemotherapy with respect to the concentration of 8-MOP in the skin and the amount of UVA received by the epidermal cells. A simple relation proved to apply for mutation induction under different treatment conditions: 5.5 X 10(-8) per J/m2 per microgram 8-MOP/ml. On this basis the mutation induction in dividing cells per session of PUVA-photochemotherapy amounts to 12.4 X 10(-5), which is probably an over-estimation.", "contents": "Mutagenicity of 8-methoxypsoralen and long-wave ultraviolet irradiation in V-79 Chinese hamster cells. A first approach to a risk estimate in photochemotherapy. The effect of 8-methoxypsoralen (8-MOP) and long-wave ultraviolet irradiation (UVA) on cell killing and mutation induction was studied in V-79 Chinese hamster cells. No effect was observed after treatment with 8-MOP alone (50 microgram/ml, 4 h), UVA alone (9000 J/m2), or 8-MOP metabolized by rat-liver microsomes. Combined treatment with 8-MOP and UVA induced both cell killing and mutation. This was also observed under conditions approaching patient treatment with PUVA photochemotherapy with respect to the concentration of 8-MOP in the skin and the amount of UVA received by the epidermal cells. A simple relation proved to apply for mutation induction under different treatment conditions: 5.5 X 10(-8) per J/m2 per microgram 8-MOP/ml. On this basis the mutation induction in dividing cells per session of PUVA-photochemotherapy amounts to 12.4 X 10(-5), which is probably an over-estimation."}
{"id": "PMID:481435", "title": "gamma-Ray mutagenesis in bacteriophage T4 is not enhanced by oxygen.", "content": "As in the induction of r mutants in bacteriophage T4 by gamma-rays, the radiation-induced reversion of T4 amber mutants to wild-type was found to depend on the product of the DNA-repair gene x of the phage. Neither the efficiency of induction of r mutants nor the efficiency of reversion of ambers was enhanced by the presence of oxygen during irradiation. T4 differed in this respect from phage T7, for which no indication has been found that gamma-ray mutagenesis results from error-prone repair of DNA damage. Notwithstanding the substantial contribution of misrepair to mutation induction in T4, the efficiency of induction per base-pair observed for irradiation under oxygen was lower than that found previously for T7.", "contents": "gamma-Ray mutagenesis in bacteriophage T4 is not enhanced by oxygen. As in the induction of r mutants in bacteriophage T4 by gamma-rays, the radiation-induced reversion of T4 amber mutants to wild-type was found to depend on the product of the DNA-repair gene x of the phage. Neither the efficiency of induction of r mutants nor the efficiency of reversion of ambers was enhanced by the presence of oxygen during irradiation. T4 differed in this respect from phage T7, for which no indication has been found that gamma-ray mutagenesis results from error-prone repair of DNA damage. Notwithstanding the substantial contribution of misrepair to mutation induction in T4, the efficiency of induction per base-pair observed for irradiation under oxygen was lower than that found previously for T7."}
{"id": "PMID:481436", "title": "Aberrations of holokinetic chromosomes and associated lethality after x-irradiation of meiotic stages in Tetranychus urticae Koch (acari, tetranychidae).", "content": "Chromosomes of the holokinetic organization type were irradiated with X-rays in various stages of meiosis in unfertilized eggs of Tetranychus urticae Koch. Visible cytological aberrations, lethality and sterility were investigated in subsequent generations. Chromosome fragments are the most frequently occurring light-microscopically visible chromosome aberrations; bridges are not formed. Contrary to expectations, the presence of fragments appears to be positively correlated with the occurrence of lethality; loss of fragments, mis-segregation and the measure of damage to the broken chromosome parts are involved. In contrast with monokinetic chromosomes the earliest lethality occurs only after about 10 divisions. The ratios between different embryonic lethality types (early vs. late) differ depending on the stage irradiated: in more compact chromatin, more serious damage (i.e. more early lethality syndromes) is induced than in less compact chromatin. In the progeny of the surviving males, neither translocations nor independent fragments are found; indirect evidence indicated the occasional presence of inversions. The presumptive inversions are induced more frequently in a chromatin-compact stage (metaphase I) than in a less compact one (telophase I).", "contents": "Aberrations of holokinetic chromosomes and associated lethality after x-irradiation of meiotic stages in Tetranychus urticae Koch (acari, tetranychidae). Chromosomes of the holokinetic organization type were irradiated with X-rays in various stages of meiosis in unfertilized eggs of Tetranychus urticae Koch. Visible cytological aberrations, lethality and sterility were investigated in subsequent generations. Chromosome fragments are the most frequently occurring light-microscopically visible chromosome aberrations; bridges are not formed. Contrary to expectations, the presence of fragments appears to be positively correlated with the occurrence of lethality; loss of fragments, mis-segregation and the measure of damage to the broken chromosome parts are involved. In contrast with monokinetic chromosomes the earliest lethality occurs only after about 10 divisions. The ratios between different embryonic lethality types (early vs. late) differ depending on the stage irradiated: in more compact chromatin, more serious damage (i.e. more early lethality syndromes) is induced than in less compact chromatin. In the progeny of the surviving males, neither translocations nor independent fragments are found; indirect evidence indicated the occasional presence of inversions. The presumptive inversions are induced more frequently in a chromatin-compact stage (metaphase I) than in a less compact one (telophase I)."}
{"id": "PMID:481437", "title": "Induction of lethal mutations in female mice by 9 generations of gamma-irradiation during foetal development.", "content": "Female CBA mice were chronically gamma-irradiated in utero during either of two periods, the 10th to 14th days or the 14th to 18th days of gestation. The doses administered were 34 rad/generation in the earlier group and 160 rad/generation in the latter with dose rates of 0.3 rad/h and 1.7 rad/h, respectively. The doses were given through 9 generations. The effect of the irradiation was expressed as an increased frequency in the rate of recessive lethal equivalents by just above 4%. This corresponds to a mutation rate of 1.5 X 10(-4) mutation/rad/genome in the animals irradiated during the 10th to 14th gestational days and 0.3 X 10(-4) mutation/rad/genome in the 14th to 18th day group. As in earlier investigations, neither dominant mutations nor dominance effects of induced recessive lethal equivalents were found.", "contents": "Induction of lethal mutations in female mice by 9 generations of gamma-irradiation during foetal development. Female CBA mice were chronically gamma-irradiated in utero during either of two periods, the 10th to 14th days or the 14th to 18th days of gestation. The doses administered were 34 rad/generation in the earlier group and 160 rad/generation in the latter with dose rates of 0.3 rad/h and 1.7 rad/h, respectively. The doses were given through 9 generations. The effect of the irradiation was expressed as an increased frequency in the rate of recessive lethal equivalents by just above 4%. This corresponds to a mutation rate of 1.5 X 10(-4) mutation/rad/genome in the animals irradiated during the 10th to 14th gestational days and 0.3 X 10(-4) mutation/rad/genome in the 14th to 18th day group. As in earlier investigations, neither dominant mutations nor dominance effects of induced recessive lethal equivalents were found."}
{"id": "PMID:481438", "title": "The effect of oxygen concentration on the X-ray induction of chromosome aberrations in human lymphocytes.", "content": "In vitro dose--response curves, for unstable chromosome aberration induction in human lymphocytes under conditions of full oxygenation or of anoxia, have been obtained using 250 kVp X-rays. Dicentric yields have been fitted to the quadratic function Y = alpha D + beta D2. An Oxygen Enhancement Ratio (OER) ranging from 7.2 to 2.7 was calculated from the coefficients of these curves possibly indicating that the data do not fit to the dose-modifying model of the oxygen effect although the differences are not statistically significant. A similar analysis of total aberration data yields an OER of 3.6 to 2.7 fitting much more satisfactorily to the dose-modifying model. Variations in dicentric yield induced by 3.0 Gy and 0.75 Gy of X-rays with increasing oxygen concentration were plotted and for each dose a constant dicentric yield was observed at oxygen levels of 2 and 250 ppm. Above 250 ppm yields increased steeply up to about 1% oxygen and then more gradually to a maximum at 100% oxygen.", "contents": "The effect of oxygen concentration on the X-ray induction of chromosome aberrations in human lymphocytes. In vitro dose--response curves, for unstable chromosome aberration induction in human lymphocytes under conditions of full oxygenation or of anoxia, have been obtained using 250 kVp X-rays. Dicentric yields have been fitted to the quadratic function Y = alpha D + beta D2. An Oxygen Enhancement Ratio (OER) ranging from 7.2 to 2.7 was calculated from the coefficients of these curves possibly indicating that the data do not fit to the dose-modifying model of the oxygen effect although the differences are not statistically significant. A similar analysis of total aberration data yields an OER of 3.6 to 2.7 fitting much more satisfactorily to the dose-modifying model. Variations in dicentric yield induced by 3.0 Gy and 0.75 Gy of X-rays with increasing oxygen concentration were plotted and for each dose a constant dicentric yield was observed at oxygen levels of 2 and 250 ppm. Above 250 ppm yields increased steeply up to about 1% oxygen and then more gradually to a maximum at 100% oxygen."}
{"id": "PMID:481439", "title": "Facilitation by pyrimidine deoxyribonucleosides and hypoxanthine of mutagenic and cytotoxic effects of monofunctional alkylating agents in Chinese hamster cells.", "content": "Hypoxanthine (Hx), thymidine (TdR) and deoxycytidine (CdR), at concentrations of 10(-5) M increased the yield of 8-azaguanine-resistant (AzGr) mutants induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in cultured Chinese hamster V79 cells. The cytotoxicity of MNNG was increased 2-fold in the presence of Hx, and more than 10-fold in the presence of TdR. This cytotoxic effect of TdR was abolished by equal concentrations of CdR, which by itself did not increase the cytotoxicity of MNNG. However, the yield of MNNG-induced AzGr colonies was increased 2--10-fold in the presence of both CdR and TdR. The AzGr colonies displayed phenotypes characteristic of hypoxanthine: guaninephosphoribosyltransferase-deficient (HGPRT-) mutants, or indicative of mutant HGPRT with altered substrate affinities. The nucleosides did not affect the growth or expression time of the HGPRT- mutants; the same extent of alkali-labile DNA damage occurred in cells treated with alkylating agents in the presence and absence of TdR and CdR; and the increase in mutation frequency in the presence of these nucleosides occurred not only with MNNG, but also with ethylating agents. Nucleosides treated with MNNG were not mutagenic, and treatment of the cells with TdR and CdR only prior to treatment with MNNG or only during selection with AzG did not increase the induced mutation frequency. Therefore, the interpretation is proposed that CdR, TdR and Hx produce nucleotide-pool imbalances that increase lethal and mutagenic errors of replication of alkylated DNA.", "contents": "Facilitation by pyrimidine deoxyribonucleosides and hypoxanthine of mutagenic and cytotoxic effects of monofunctional alkylating agents in Chinese hamster cells. Hypoxanthine (Hx), thymidine (TdR) and deoxycytidine (CdR), at concentrations of 10(-5) M increased the yield of 8-azaguanine-resistant (AzGr) mutants induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in cultured Chinese hamster V79 cells. The cytotoxicity of MNNG was increased 2-fold in the presence of Hx, and more than 10-fold in the presence of TdR. This cytotoxic effect of TdR was abolished by equal concentrations of CdR, which by itself did not increase the cytotoxicity of MNNG. However, the yield of MNNG-induced AzGr colonies was increased 2--10-fold in the presence of both CdR and TdR. The AzGr colonies displayed phenotypes characteristic of hypoxanthine: guaninephosphoribosyltransferase-deficient (HGPRT-) mutants, or indicative of mutant HGPRT with altered substrate affinities. The nucleosides did not affect the growth or expression time of the HGPRT- mutants; the same extent of alkali-labile DNA damage occurred in cells treated with alkylating agents in the presence and absence of TdR and CdR; and the increase in mutation frequency in the presence of these nucleosides occurred not only with MNNG, but also with ethylating agents. Nucleosides treated with MNNG were not mutagenic, and treatment of the cells with TdR and CdR only prior to treatment with MNNG or only during selection with AzG did not increase the induced mutation frequency. Therefore, the interpretation is proposed that CdR, TdR and Hx produce nucleotide-pool imbalances that increase lethal and mutagenic errors of replication of alkylated DNA."}
{"id": "PMID:481440", "title": "The recovery of mammalian cells treated with methyl methanesulfonate, nitrogen mustard or UV light. I. The effect of alkylation products on DNA replication.", "content": "CHO cells were synchronized in G1 phase and treated with MMS or HN2. The subsequent rate of DNA replication was found to be reduced in a dose-dependent manner. In addition, 2 X 10(-3 M and 3 X 10(-3) M MMS resulted in a 3--4 h delay prior to the initiation of S phase. If the cells were held for 8 h in hydroxyurea after MMS treatment, no subsequent lag in DNA synthesis was seen after removal of the hydroxyurea. The entry of confluent cells into S phase was found to be delayed 7 h upon trypsinizing and replating. Treatment of these cells with MMS resulted in a reduced rate of DNA replication, but no further delay in its initiation. Repair replication was found to continue at a constant rate for at least 12 h following MMS treatment of cells under all of these conditions. At the concentrations used in these experiments MMS severely inhibited the rate of protein synthesis, but HN2 had little effect. By comparing both the kinetics of repair replication and recovery of protein synthesis with the rate of DNA replication, it was concluded that the initial, severe reduction in rate following MMS treatment was probably due to an inhibition of protein synthesis.", "contents": "The recovery of mammalian cells treated with methyl methanesulfonate, nitrogen mustard or UV light. I. The effect of alkylation products on DNA replication. CHO cells were synchronized in G1 phase and treated with MMS or HN2. The subsequent rate of DNA replication was found to be reduced in a dose-dependent manner. In addition, 2 X 10(-3 M and 3 X 10(-3) M MMS resulted in a 3--4 h delay prior to the initiation of S phase. If the cells were held for 8 h in hydroxyurea after MMS treatment, no subsequent lag in DNA synthesis was seen after removal of the hydroxyurea. The entry of confluent cells into S phase was found to be delayed 7 h upon trypsinizing and replating. Treatment of these cells with MMS resulted in a reduced rate of DNA replication, but no further delay in its initiation. Repair replication was found to continue at a constant rate for at least 12 h following MMS treatment of cells under all of these conditions. At the concentrations used in these experiments MMS severely inhibited the rate of protein synthesis, but HN2 had little effect. By comparing both the kinetics of repair replication and recovery of protein synthesis with the rate of DNA replication, it was concluded that the initial, severe reduction in rate following MMS treatment was probably due to an inhibition of protein synthesis."}
{"id": "PMID:481441", "title": "The recovery of mammalian cells treated with methyl methanesolfonate, nitrogen mustard or UV light. II. The importance of DNA repair prior to the initiation of S phase.", "content": "CHO cells were synchronized 2 G1 phase and treated with UV light or HN2. These treatments resulted in a dose-dependent reduction in the rate of DNA replication and cell survival. Holding UV-irradiated cells in G1 phase (in HU medium) for an additional 10 h prior to their release into S phase did not assist recovery as measured by either of these criteria. The survival of cells treated with HN2 was also not enhanced by this recovery period. However, following 2 X 10(-5) M HN2 the rate of DNA replication increased from 30% to 70% of the control level when the period in HU medium was extended to 14 h. The induction of cross-links following HN2 treatment of asynchronous cells was shown to be dose dependent. Subsequent incubation in fresh medium resulted in complete recovery within 20 h at concentrations of HN2 up to 10(-5) M, and at 2 X 10(-5) M HN2, 75% of the cross-links were removed at 14 h post treatment.", "contents": "The recovery of mammalian cells treated with methyl methanesolfonate, nitrogen mustard or UV light. II. The importance of DNA repair prior to the initiation of S phase. CHO cells were synchronized 2 G1 phase and treated with UV light or HN2. These treatments resulted in a dose-dependent reduction in the rate of DNA replication and cell survival. Holding UV-irradiated cells in G1 phase (in HU medium) for an additional 10 h prior to their release into S phase did not assist recovery as measured by either of these criteria. The survival of cells treated with HN2 was also not enhanced by this recovery period. However, following 2 X 10(-5) M HN2 the rate of DNA replication increased from 30% to 70% of the control level when the period in HU medium was extended to 14 h. The induction of cross-links following HN2 treatment of asynchronous cells was shown to be dose dependent. Subsequent incubation in fresh medium resulted in complete recovery within 20 h at concentrations of HN2 up to 10(-5) M, and at 2 X 10(-5) M HN2, 75% of the cross-links were removed at 14 h post treatment."}
{"id": "PMID:481442", "title": "Superoxide dismutase and catalase activities in Ataxia telangiectasia and normal fibroblast cell extracts.", "content": "Superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) are important enzymes involved in protection of the cell from harmful effects of oxidative degradation. The respective substrates for these enzymes, superoxide anion and hydrogen peroxide, can be generated within the cell either by normal metabolism or by ionizing radiation. The hypothesis that the inherent radiosensitivity associated with the human autosomal recessive disease Ataxia telangiectasia is due to decreased levels of SOD and/or catalase was tested. The results suggest that fibroblast cells derived from ataxia patients are normal with respect to these two enzymes.", "contents": "Superoxide dismutase and catalase activities in Ataxia telangiectasia and normal fibroblast cell extracts. Superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) are important enzymes involved in protection of the cell from harmful effects of oxidative degradation. The respective substrates for these enzymes, superoxide anion and hydrogen peroxide, can be generated within the cell either by normal metabolism or by ionizing radiation. The hypothesis that the inherent radiosensitivity associated with the human autosomal recessive disease Ataxia telangiectasia is due to decreased levels of SOD and/or catalase was tested. The results suggest that fibroblast cells derived from ataxia patients are normal with respect to these two enzymes."}
{"id": "PMID:481447", "title": "Induction of 8-azaguanine- and ouabain-resistant mutants by cyclophosphamide and 1-(pyridyl-3)-3,3-dimethyltriazene in Chinese hamster cells cultured in diffusion chambers in mice.", "content": "A host-mediated assay is described for induction of 8-azaguanine-resistant (azgr) and ouabain-resistant (ouar) mutants in Chinese hamster V79 cells cultured in diffusion chambers (DC) in C3H mice. Injection of the hosts with the indirect mutagen/carcinogen cyclophosphamide (CPP) or 1-(pyridyl-3)-3,3-dimethyltriazene (PyDT) caused a dose-dependent increase in mutation frequency at the loci of azgr and ouar in the V79 target cells. Plating efficiency of V79 cells in DC in mice was decreased depending upon the dose of CPP or PyDT given to the hosts. In addition, the relationship between expression time and mutation frequency was examined and discussed. The data support the use of this system as an effective screening procedure for suspected environmental mutagens or carcinogens, especially those that need to be metabolically activated in vivo.", "contents": "Induction of 8-azaguanine- and ouabain-resistant mutants by cyclophosphamide and 1-(pyridyl-3)-3,3-dimethyltriazene in Chinese hamster cells cultured in diffusion chambers in mice. A host-mediated assay is described for induction of 8-azaguanine-resistant (azgr) and ouabain-resistant (ouar) mutants in Chinese hamster V79 cells cultured in diffusion chambers (DC) in C3H mice. Injection of the hosts with the indirect mutagen/carcinogen cyclophosphamide (CPP) or 1-(pyridyl-3)-3,3-dimethyltriazene (PyDT) caused a dose-dependent increase in mutation frequency at the loci of azgr and ouar in the V79 target cells. Plating efficiency of V79 cells in DC in mice was decreased depending upon the dose of CPP or PyDT given to the hosts. In addition, the relationship between expression time and mutation frequency was examined and discussed. The data support the use of this system as an effective screening procedure for suspected environmental mutagens or carcinogens, especially those that need to be metabolically activated in vivo."}
{"id": "PMID:481448", "title": "Inducibility of chromosomal aberrations by metal compounds in cultured mammalian cells.", "content": "Metal compounds were tested for their ability to induce chromosomal aberrations in cultured mammalian cells. Chromosomal aberrations were induced by the application of some Cr, Mn and Ni compounds. Among 6-valent Cr compounds, K2Cr2O7 and CrO3 induced high levels of aberrations, at rates which were similar for Cr-equivalent doses. The perchromate compounds were more efficient in producing chromosomal aberrations than was a chromate compound, K2CrO4. A 3-valent Cr compound, Cr2(SO4)3, was less toxic and failed to induce a demonstrable increase in chromosomal aberrations. KMnO4 induced aberrations, but at a low rate. As to Ni compounds, NiCl2 and (CH3COO)2Ni induced few aberrations. Administration of K2Ni(CN)4 induced only gaps. NiS induced a low but definite increase in chromosomal aberrations. The rate of these aberrations increased with an increase in treatment time from 24 to 48 h, indicating a time-dependent increase in the hereditable toxicity of metal compounds. CdCl2 and HgCl2 were somewhat toxic, but failed to induce chromosomal aberrations in the present study.", "contents": "Inducibility of chromosomal aberrations by metal compounds in cultured mammalian cells. Metal compounds were tested for their ability to induce chromosomal aberrations in cultured mammalian cells. Chromosomal aberrations were induced by the application of some Cr, Mn and Ni compounds. Among 6-valent Cr compounds, K2Cr2O7 and CrO3 induced high levels of aberrations, at rates which were similar for Cr-equivalent doses. The perchromate compounds were more efficient in producing chromosomal aberrations than was a chromate compound, K2CrO4. A 3-valent Cr compound, Cr2(SO4)3, was less toxic and failed to induce a demonstrable increase in chromosomal aberrations. KMnO4 induced aberrations, but at a low rate. As to Ni compounds, NiCl2 and (CH3COO)2Ni induced few aberrations. Administration of K2Ni(CN)4 induced only gaps. NiS induced a low but definite increase in chromosomal aberrations. The rate of these aberrations increased with an increase in treatment time from 24 to 48 h, indicating a time-dependent increase in the hereditable toxicity of metal compounds. CdCl2 and HgCl2 were somewhat toxic, but failed to induce chromosomal aberrations in the present study."}
{"id": "PMID:481449", "title": "Chromosomal aberrations and sister-chromatid exchanges in Chinese hamster cells treated in vitro with hexavalent chromium compounds.", "content": "Chinese hamster cells (CHO line) were treated in vitro for 30--39 h with hexavalent chromium compounds (K2Cr2O7 and Na2CrO7), at concentrations ranging from 0.1 to 1.0 microgram of Cr6+ per ml, in medium containing BUdr. Chromosomal aberrations and sister-chromatid exchanges were scored on BUdr-labelled 2nd division metaphases, collected at the end of treatment and stained with Giemsa. Treatment with mitomycin C 0.009--0.030 microgram/ml) was carried out as a control for the responsiveness of the cell system to chromosomal damage. Both chromium compounds induced marked mitotic delays. Chromosomal aberrations were increased about 10-fold by exposure to Cr6+ (1.0 microgram/ml). The principal aberrations observed were single chromatid gaps, breaks and interchanges, whose frequencies increased proportionally to the concentration of chromium. Dicentric chromosomes, isochromatid breaks, chromosome and chromatid rings were also induced. The frequenyc of sister-chromatid exchanges was hardly doubled 30 h after exposure to Cr6+ at 0.3 microgram/ml, whereas it was trebled 39 h after treatment, in the cells whose division cycle had been slowed down by chromium.", "contents": "Chromosomal aberrations and sister-chromatid exchanges in Chinese hamster cells treated in vitro with hexavalent chromium compounds. Chinese hamster cells (CHO line) were treated in vitro for 30--39 h with hexavalent chromium compounds (K2Cr2O7 and Na2CrO7), at concentrations ranging from 0.1 to 1.0 microgram of Cr6+ per ml, in medium containing BUdr. Chromosomal aberrations and sister-chromatid exchanges were scored on BUdr-labelled 2nd division metaphases, collected at the end of treatment and stained with Giemsa. Treatment with mitomycin C 0.009--0.030 microgram/ml) was carried out as a control for the responsiveness of the cell system to chromosomal damage. Both chromium compounds induced marked mitotic delays. Chromosomal aberrations were increased about 10-fold by exposure to Cr6+ (1.0 microgram/ml). The principal aberrations observed were single chromatid gaps, breaks and interchanges, whose frequencies increased proportionally to the concentration of chromium. Dicentric chromosomes, isochromatid breaks, chromosome and chromatid rings were also induced. The frequenyc of sister-chromatid exchanges was hardly doubled 30 h after exposure to Cr6+ at 0.3 microgram/ml, whereas it was trebled 39 h after treatment, in the cells whose division cycle had been slowed down by chromium."}
{"id": "PMID:481450", "title": "Cytogenetic activity of some common antioxidants and their interaction with x-rays.", "content": "Experiments were designed to investigate the cytogenetic activity of some commonly used phenolic antioxidants, namely BHA, BHT, BMP and PG, all used widely to preserve and stablize food products rich in fats and oils. Barley and onion were used as test systems. The treatments reduced seed germination and induced a significant amount of seedling injury. Only PMP treatments produced a moderate amount of chromosomal aberrations while others failed to do so. Being strong antioxidants it was expected that these compounds would reduce the cytogenetic damage caused by the radiations. Contrary to expectations, however, a significant increase was observed in the radiation damage measured as seed and seedling lethality and chromosomal aberrations. Chemical post-treatments also increased the radiation damage. The damage was, however, reduced with ATP post-irradiation treatment.", "contents": "Cytogenetic activity of some common antioxidants and their interaction with x-rays. Experiments were designed to investigate the cytogenetic activity of some commonly used phenolic antioxidants, namely BHA, BHT, BMP and PG, all used widely to preserve and stablize food products rich in fats and oils. Barley and onion were used as test systems. The treatments reduced seed germination and induced a significant amount of seedling injury. Only PMP treatments produced a moderate amount of chromosomal aberrations while others failed to do so. Being strong antioxidants it was expected that these compounds would reduce the cytogenetic damage caused by the radiations. Contrary to expectations, however, a significant increase was observed in the radiation damage measured as seed and seedling lethality and chromosomal aberrations. Chemical post-treatments also increased the radiation damage. The damage was, however, reduced with ATP post-irradiation treatment."}
{"id": "PMID:481451", "title": "Chromosomal aberrations induced by maleic hydrazide and related compounds in Chinese hamster cells in vitro.", "content": "The cytotoxic effects and chromosomal abnormalities induced by maleic hydrazide (MH) and its salts were investigated in cultured V79 cells. MH, and its potassium (K-MH) and diethanolamine (DEA-MH) salts were tested. MH was 5--14 times more cytotoxic than its salts and almost 4.5 times less toxic than the related compound, hydrazine dihydrochloride (HDC). MH salts had very weak cytotoxicity; the LD50 values on V79 cells on exposure for 3 h in vitro were (in microgram/ml) 1100 (MH), 12 000 (DEA-MH), 20 000 (K-MH), 230 (HDC) and 10 000 (NaCl). Both MH and its salts--but neither HDC nor NaCl--caused chromosomal aberrations in cultured V79 cells. The maximal frequencies of aberrant cells in cultures exposed to the compounds for 3 h in vitro were 18% (mh at 100 microgram/ml), 18% (K-MH at 20 000 microgram/ml) and 13% (DEA-MH at 20 000 microgram/ml). Maximal frequencies observed in cultures treated with HDC or NaCl were 10% (HDC at 400 microgram/ml) and 5% NaCl at 10 000 microgram/ml). Those of positive groups were 97% (N-methyl-N'-nitro-N-nitrosoguanidine, MNNG, at 5 microgram/ml) and 16% (ethyl methanesulfonate, EMS, at 400 microgram/ml). These frequencies of MH and its salts were 3.25--4.5 times those in untreated control cells. These results suggested that MH and its salts had weak inducibili", "contents": "Chromosomal aberrations induced by maleic hydrazide and related compounds in Chinese hamster cells in vitro. The cytotoxic effects and chromosomal abnormalities induced by maleic hydrazide (MH) and its salts were investigated in cultured V79 cells. MH, and its potassium (K-MH) and diethanolamine (DEA-MH) salts were tested. MH was 5--14 times more cytotoxic than its salts and almost 4.5 times less toxic than the related compound, hydrazine dihydrochloride (HDC). MH salts had very weak cytotoxicity; the LD50 values on V79 cells on exposure for 3 h in vitro were (in microgram/ml) 1100 (MH), 12 000 (DEA-MH), 20 000 (K-MH), 230 (HDC) and 10 000 (NaCl). Both MH and its salts--but neither HDC nor NaCl--caused chromosomal aberrations in cultured V79 cells. The maximal frequencies of aberrant cells in cultures exposed to the compounds for 3 h in vitro were 18% (mh at 100 microgram/ml), 18% (K-MH at 20 000 microgram/ml) and 13% (DEA-MH at 20 000 microgram/ml). Maximal frequencies observed in cultures treated with HDC or NaCl were 10% (HDC at 400 microgram/ml) and 5% NaCl at 10 000 microgram/ml). Those of positive groups were 97% (N-methyl-N'-nitro-N-nitrosoguanidine, MNNG, at 5 microgram/ml) and 16% (ethyl methanesulfonate, EMS, at 400 microgram/ml). These frequencies of MH and its salts were 3.25--4.5 times those in untreated control cells. These results suggested that MH and its salts had weak inducibili"}
{"id": "PMID:481452", "title": "Mutagenicity, cytotoxicity and DNA crosslinking in V79 Chinese hamster cells treated with cis- and trans-Pt(II) diamminedichloride.", "content": "The mutagenicity and cytotoxicity of cis- and trans-Pt(II) diamminedichloride (PDD) were examined in V79 Chinese hamster lung cells and compared with effects on DNA measured by alkaline elution. DNA--protein crosslinks and DNA interstrand crosslinks were detected following doses of cis-PDD which reduced cell survival 80--90% and which produced a mutant frequency of 3 X 10(-4) at the HGPRT locus. Equitoxic doses of trans-PDD were much less mutagenic than cis-PDD. At equitoxic doses, trans-PDD produced more DNA-protein crosslinking than did cis-PDD, but interstrand crosslinking for the two isomers was comparable. Hence, the interstrand crosslink could be the cytotoxic lesion produced by these Pt compounds whereas neither of these DNA lesions are necessarily mutagenic. The mutagenesis produced by cis-PDD could be due to crosslinks of a different type than those produced by trans-PDD or it may be due to monofunctional damage.", "contents": "Mutagenicity, cytotoxicity and DNA crosslinking in V79 Chinese hamster cells treated with cis- and trans-Pt(II) diamminedichloride. The mutagenicity and cytotoxicity of cis- and trans-Pt(II) diamminedichloride (PDD) were examined in V79 Chinese hamster lung cells and compared with effects on DNA measured by alkaline elution. DNA--protein crosslinks and DNA interstrand crosslinks were detected following doses of cis-PDD which reduced cell survival 80--90% and which produced a mutant frequency of 3 X 10(-4) at the HGPRT locus. Equitoxic doses of trans-PDD were much less mutagenic than cis-PDD. At equitoxic doses, trans-PDD produced more DNA-protein crosslinking than did cis-PDD, but interstrand crosslinking for the two isomers was comparable. Hence, the interstrand crosslink could be the cytotoxic lesion produced by these Pt compounds whereas neither of these DNA lesions are necessarily mutagenic. The mutagenesis produced by cis-PDD could be due to crosslinks of a different type than those produced by trans-PDD or it may be due to monofunctional damage."}
{"id": "PMID:481453", "title": "Absence of genotoxic effects of metronidazole and two of its urinary metabolites on human lymphocytes in vitro.", "content": "The antiprotozoan agent metronidazole (1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole) and two of its major human urinary excretion products, 2-methyl-5-nitromidazole-1-yl acetic acid and 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole were tested for genotoxic activity in human lymphocytes in vitro by analysis of chromosome aberrations, sister-chromatid exchanges and DNA-repair synthesis. The positive control compounds methyl methanesulphonate (MMS) and nitrogen mustard (HN2) showed significant genotoxic activity in these tests. No such activity of metronidazole and its two metabolites was detected in concentrations up to 1000 microgram/ml (5.8 X 10(-3) M). Nor did these 3 compounds influence DNA-repair synthesis induced by MMS and HN2. These results suggest that metronidazole, 2-methyl-5-nitroimidazole-1-yl acetic acid and 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole have no direct genotoxic effect on human lymphocytes in vitro.", "contents": "Absence of genotoxic effects of metronidazole and two of its urinary metabolites on human lymphocytes in vitro. The antiprotozoan agent metronidazole (1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole) and two of its major human urinary excretion products, 2-methyl-5-nitromidazole-1-yl acetic acid and 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole were tested for genotoxic activity in human lymphocytes in vitro by analysis of chromosome aberrations, sister-chromatid exchanges and DNA-repair synthesis. The positive control compounds methyl methanesulphonate (MMS) and nitrogen mustard (HN2) showed significant genotoxic activity in these tests. No such activity of metronidazole and its two metabolites was detected in concentrations up to 1000 microgram/ml (5.8 X 10(-3) M). Nor did these 3 compounds influence DNA-repair synthesis induced by MMS and HN2. These results suggest that metronidazole, 2-methyl-5-nitroimidazole-1-yl acetic acid and 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole have no direct genotoxic effect on human lymphocytes in vitro."}
{"id": "PMID:481454", "title": "Sister-chromatid exchanges and chromosal breakage in patients treated with cytostatics.", "content": "The frequency of structural chromosomal rearrangements and sister-chromatid exchanges (SCEs) was investigated in short-term phytohemagglutininstimulated lymphocyte cultures by means of bromodeoxyuridine substitution and fluorescence plus Giemsa (FPG) staining technique. Both these parameters were significantly increased in patients treated with comparatively low doses of cyclophosphamide, busulphan and adriamycin. The increased SCE rate was proportional to the number of chromosome breaks, the ratio of SCE to breaks being about 100:1. The increase in the SCE number was maintained for several months after the termination of cytostatic therapy, when the conventional analysis of chromosome breaks yielded normal results. Normal SCE values were obtained in two patients treated with low doses of fluorouracil.", "contents": "Sister-chromatid exchanges and chromosal breakage in patients treated with cytostatics. The frequency of structural chromosomal rearrangements and sister-chromatid exchanges (SCEs) was investigated in short-term phytohemagglutininstimulated lymphocyte cultures by means of bromodeoxyuridine substitution and fluorescence plus Giemsa (FPG) staining technique. Both these parameters were significantly increased in patients treated with comparatively low doses of cyclophosphamide, busulphan and adriamycin. The increased SCE rate was proportional to the number of chromosome breaks, the ratio of SCE to breaks being about 100:1. The increase in the SCE number was maintained for several months after the termination of cytostatic therapy, when the conventional analysis of chromosome breaks yielded normal results. Normal SCE values were obtained in two patients treated with low doses of fluorouracil."}
{"id": "PMID:481456", "title": "Urethane and hydroxyurethane induce sister-chromatid exchanges in cultured human lymphocytes.", "content": "Both urethane and hydroxyurethane induced sister-chromatid exchanges (SCE) in cultured human lymphocytes. Aroclor-induced rat-liver microsome fraction deactivated rather than activated these two agents in the lymphocyte system.", "contents": "Urethane and hydroxyurethane induce sister-chromatid exchanges in cultured human lymphocytes. Both urethane and hydroxyurethane induced sister-chromatid exchanges (SCE) in cultured human lymphocytes. Aroclor-induced rat-liver microsome fraction deactivated rather than activated these two agents in the lymphocyte system."}
{"id": "PMID:481457", "title": "Activity of citrinin metabolized by rat and human microsome fractions in clastogenicity and SCE assays on Chinese hamster V79-E cells.", "content": "The mycotoxin citrinin is a potent inducer of chromosomal aberrations in the clastogenicity assay on V79-E cells when metabolized by rat and human liver microsomes. Rat and human liver microsomes, standardized on protein content, activate citrinin at equal levels. 5 X 10(-4) M citrinin induces complex translocations in a high frequency as well as defects of chromosomal coiling. Higher concentrations are cytotoxic, lower ones are almost inactive. After metabolization of mycotoxin by rat-kidney microsomes or an S9 mix fraction containing rat liver and kidney microsomes, toxic effects predominate and chromosomal aberrations are diminished. Clastogenic citrinin concentrations do not induce an increase of SCE frequency. Although the mode of action of this mycotoxin on chromosomal structure remains obscure, possible explanations are discussed.", "contents": "Activity of citrinin metabolized by rat and human microsome fractions in clastogenicity and SCE assays on Chinese hamster V79-E cells. The mycotoxin citrinin is a potent inducer of chromosomal aberrations in the clastogenicity assay on V79-E cells when metabolized by rat and human liver microsomes. Rat and human liver microsomes, standardized on protein content, activate citrinin at equal levels. 5 X 10(-4) M citrinin induces complex translocations in a high frequency as well as defects of chromosomal coiling. Higher concentrations are cytotoxic, lower ones are almost inactive. After metabolization of mycotoxin by rat-kidney microsomes or an S9 mix fraction containing rat liver and kidney microsomes, toxic effects predominate and chromosomal aberrations are diminished. Clastogenic citrinin concentrations do not induce an increase of SCE frequency. Although the mode of action of this mycotoxin on chromosomal structure remains obscure, possible explanations are discussed."}
{"id": "PMID:481458", "title": "Rubber solvent: a clastogenic agent that fails to induce sister-chromatid exchanges.", "content": "Rubber solvent was tested for its ability to induce chromosome aberrations and sister-chromatid exchanges in human whole blood cultures. Following exposure to relatively low rubber solvent concentrations (0.0125% and greater) significant increases in the frequencies of chromatid gaps and breaks were observed. At higher rubber-solvent concentrations (0.05% and greater) there were also significant increases in the frequency of chromosome breaks. In contrast to the increase in chromosome aberrations following rubber-solvent exposure, rubber-solvent concentrations up to the toxic level failed to produce increases in the sister-chromatid exchange frequency.", "contents": "Rubber solvent: a clastogenic agent that fails to induce sister-chromatid exchanges. Rubber solvent was tested for its ability to induce chromosome aberrations and sister-chromatid exchanges in human whole blood cultures. Following exposure to relatively low rubber solvent concentrations (0.0125% and greater) significant increases in the frequencies of chromatid gaps and breaks were observed. At higher rubber-solvent concentrations (0.05% and greater) there were also significant increases in the frequency of chromosome breaks. In contrast to the increase in chromosome aberrations following rubber-solvent exposure, rubber-solvent concentrations up to the toxic level failed to produce increases in the sister-chromatid exchange frequency."}
{"id": "PMID:481459", "title": "The effect of a tumor promotor, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), on sister-chromatid exchange formation in cultured Chinese hamster cells.", "content": "The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) does not increase the sister-chromatid exchange (SCE) frequency in either Chinese hamster ovary (CHO) or lung (V 79) cells which are cultured in the presence of 5-bromodeoxyuridine. Moreover, TPA does not alter the induction of SCEs in CHO cells by mitomycin C during the first 3 cycles following addition of the alkylating agent. These SCE induction data do not by themselves support the hypothesis that tumor promotion by TPA depends on the enhancement of mitotic recombination.", "contents": "The effect of a tumor promotor, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), on sister-chromatid exchange formation in cultured Chinese hamster cells. The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) does not increase the sister-chromatid exchange (SCE) frequency in either Chinese hamster ovary (CHO) or lung (V 79) cells which are cultured in the presence of 5-bromodeoxyuridine. Moreover, TPA does not alter the induction of SCEs in CHO cells by mitomycin C during the first 3 cycles following addition of the alkylating agent. These SCE induction data do not by themselves support the hypothesis that tumor promotion by TPA depends on the enhancement of mitotic recombination."}
{"id": "PMID:481461", "title": "A prospective study of spontaneous fetal losses after induced abortions.", "content": "The incidence of spontaneous abortions was observed among 31,917 women followed from their first prenatal visit. Life-table analysis showed that losses in the first trimester were not significantly affected by previous induced abortions, nor was any change in the risk of second-trimester losses detected among the 1493 parous women who reported having had induced abortions after childbirth. There was, however, an increase in the incidence of midtrimester losses among the 2019 nulliparous women with previous induced abortions; the age-adjusted rate of loss was 59.9 per 100,000 women at risk per day, as compared with 24.2 among the 12,042 control nulliparous women (P less than 0.001). The relative risk increased with the number of previous induced abortions and was not explained by the distribution of demographic and social variables. The risk decreased from 3.27 (95 per cent confidence limits, 1.72 to 6.23) after abortions induced before 1973, mainly by dilation and curettage, to 1.42 (0.76 to 2.65) after those done since 1973, when the more gentle technic of cervical dilation by use of laminaria was introduced. These findings indicate that there is little or no risk of spontaneous abortions after induced abortions when performed by current technics.", "contents": "A prospective study of spontaneous fetal losses after induced abortions. The incidence of spontaneous abortions was observed among 31,917 women followed from their first prenatal visit. Life-table analysis showed that losses in the first trimester were not significantly affected by previous induced abortions, nor was any change in the risk of second-trimester losses detected among the 1493 parous women who reported having had induced abortions after childbirth. There was, however, an increase in the incidence of midtrimester losses among the 2019 nulliparous women with previous induced abortions; the age-adjusted rate of loss was 59.9 per 100,000 women at risk per day, as compared with 24.2 among the 12,042 control nulliparous women (P less than 0.001). The relative risk increased with the number of previous induced abortions and was not explained by the distribution of demographic and social variables. The risk decreased from 3.27 (95 per cent confidence limits, 1.72 to 6.23) after abortions induced before 1973, mainly by dilation and curettage, to 1.42 (0.76 to 2.65) after those done since 1973, when the more gentle technic of cervical dilation by use of laminaria was introduced. These findings indicate that there is little or no risk of spontaneous abortions after induced abortions when performed by current technics."}
{"id": "PMID:481462", "title": "Circulating and urinary catecholamines in pheochromocytoma. Diagnostic and pathophysiologic implications.", "content": "Three biochemical tests for the diagnosis of pheochromocytoma were evaluated in 24 patients with proved tumors and 40 patients whose clinical picture was suspect but who had no evidence of the disease. Measurement of resting, supine plasma catecholamines (by radioenzymatic assay) was more useful than either 24-hour urinary vanillylmandelic acid (VMA) or metanephrines or both. In only one of 23 patients with pheochromocytoma were plasma catecholamines within the range of those in patients without pheochromocytoma, as compared with urinary VMA in 11 of 22, urinary metanephrines in five of 22 and both metabolites in three of 22. These studies reaffirm the value of plasma catecholamines in the diagnosis of pheochromocytoma and indicate that urinary catecholamine metabolites are less useful. The poor correlation between the height of arterial pressure and circulating levels of catecholamines suggests that the regulation of arterial pressure in pheochromocytoma is complex.", "contents": "Circulating and urinary catecholamines in pheochromocytoma. Diagnostic and pathophysiologic implications. Three biochemical tests for the diagnosis of pheochromocytoma were evaluated in 24 patients with proved tumors and 40 patients whose clinical picture was suspect but who had no evidence of the disease. Measurement of resting, supine plasma catecholamines (by radioenzymatic assay) was more useful than either 24-hour urinary vanillylmandelic acid (VMA) or metanephrines or both. In only one of 23 patients with pheochromocytoma were plasma catecholamines within the range of those in patients without pheochromocytoma, as compared with urinary VMA in 11 of 22, urinary metanephrines in five of 22 and both metabolites in three of 22. These studies reaffirm the value of plasma catecholamines in the diagnosis of pheochromocytoma and indicate that urinary catecholamine metabolites are less useful. The poor correlation between the height of arterial pressure and circulating levels of catecholamines suggests that the regulation of arterial pressure in pheochromocytoma is complex."}
{"id": "PMID:481481", "title": "The chemotherapy on plasma-cell myeloma and the incidence of acute leukemia.", "content": "Previously untreated patients with myeloma were randomized to initial treatment with melphalan and prednisone (and to cyclophosphamide or carmustine if relapse or progression occurred)(Group A, 125 patients), melphalan, cyclophosphamide, carmustine and prednisone in alternating (Group B, 123 patients) or concurrent (Group C, 116 patients) schedules. The groups were similar with respect to known prognostic factors. Response rates and survival were also similar. We were unable to identify a subgroup of patients who responded or survived better on melphalan-cyclophosphamide-carmustine and prednisone than on melphalan and prednisone. We conclude that the combination of the four drugs is not better than melphalen and prednisone for inducing responses or prolonging the survival of patients with myeloma. Myelomas producing only gamma chains have a poorer prognosis (P greater than 0.001) than IgG, IgA, or kappa myeloma. Acute leukemia has developed in 14 patients. The actuarial risk of developing acute leukemia, has increased rapidly to 17.4 per cent at 50 months.", "contents": "The chemotherapy on plasma-cell myeloma and the incidence of acute leukemia. Previously untreated patients with myeloma were randomized to initial treatment with melphalan and prednisone (and to cyclophosphamide or carmustine if relapse or progression occurred)(Group A, 125 patients), melphalan, cyclophosphamide, carmustine and prednisone in alternating (Group B, 123 patients) or concurrent (Group C, 116 patients) schedules. The groups were similar with respect to known prognostic factors. Response rates and survival were also similar. We were unable to identify a subgroup of patients who responded or survived better on melphalan-cyclophosphamide-carmustine and prednisone than on melphalan and prednisone. We conclude that the combination of the four drugs is not better than melphalen and prednisone for inducing responses or prolonging the survival of patients with myeloma. Myelomas producing only gamma chains have a poorer prognosis (P greater than 0.001) than IgG, IgA, or kappa myeloma. Acute leukemia has developed in 14 patients. The actuarial risk of developing acute leukemia, has increased rapidly to 17.4 per cent at 50 months."}
{"id": "PMID:481511", "title": "Streptokinase in acute myocardial infarction.", "content": "In a controlled trial of streptokinase in acute myocardial infarction, 512 of 2338 patients at 11 European centers were stratified according to clinical severity. Three hundred fifteen patients allocated to medium-risk and high-risk groups were randomized to a 24-hour infusion of streptokinase or glucose. There were no essential differences in the severity of illness in the two groups before infusion. The overall mortality rates within six months were significantly lower (P less than 0.01) in the streptokinase group (15.6 per cent) than in the control group (30.6 per cent). Bleeding complications were more frequent in the streptokinase group; except for two nonfatal cerebral hemorrhages these complications were clinically unimportant. The treatment was generally well tolerated. We tolerated. We conclude that streptokinase given under the conditions of this trial -- to medium-risk patients admitted to a coronary-care unit within 12 hours of onset of symptoms -- reduces mortality at six months. (N Engl J Med 301:797-802, 1979)", "contents": "Streptokinase in acute myocardial infarction. In a controlled trial of streptokinase in acute myocardial infarction, 512 of 2338 patients at 11 European centers were stratified according to clinical severity. Three hundred fifteen patients allocated to medium-risk and high-risk groups were randomized to a 24-hour infusion of streptokinase or glucose. There were no essential differences in the severity of illness in the two groups before infusion. The overall mortality rates within six months were significantly lower (P less than 0.01) in the streptokinase group (15.6 per cent) than in the control group (30.6 per cent). Bleeding complications were more frequent in the streptokinase group; except for two nonfatal cerebral hemorrhages these complications were clinically unimportant. The treatment was generally well tolerated. We tolerated. We conclude that streptokinase given under the conditions of this trial -- to medium-risk patients admitted to a coronary-care unit within 12 hours of onset of symptoms -- reduces mortality at six months. (N Engl J Med 301:797-802, 1979)"}
{"id": "PMID:481512", "title": "HLA-DR specificities among black Americans with juvenile-onset diabetes.", "content": "To study the association of histocompatibility (HLA) genes in black persons with juvenile-onset diabetes, we determined HLA-A, HLA-B, HLA-C and HLA-DR specificities in 40 black Americans with this disease and in 67 unaffected black Americans. Marked increases in the frequencies of HLA-DRw3 and HLA-DRw4 were found in the patients as compared with the unaffected persons: DRw3 was found in 72.5 per cent of patients versus 29.9 per cent of unaffected persons and DRw4 in 72.5 per cent versus 25.4 per cent (corrected P values each less than 0.0007). DRw2 was not found in any of the patients but was present in 26.9 per cent of unaffected persons (P corrected less than 0.035). There is thus a negative correlation between this specificity and juvenile-onset diabetes. By contrast, no meaningful differences were found in the frequencies of A, B, or C locus antigens. Studies in white persons with juvenile-onset diabetes have suggested that the reported HLA-B associations are due to HLA-D region specificities, and our results also support the premise that D region specificities are the primary associations with juvenile-onset diabetes.", "contents": "HLA-DR specificities among black Americans with juvenile-onset diabetes. To study the association of histocompatibility (HLA) genes in black persons with juvenile-onset diabetes, we determined HLA-A, HLA-B, HLA-C and HLA-DR specificities in 40 black Americans with this disease and in 67 unaffected black Americans. Marked increases in the frequencies of HLA-DRw3 and HLA-DRw4 were found in the patients as compared with the unaffected persons: DRw3 was found in 72.5 per cent of patients versus 29.9 per cent of unaffected persons and DRw4 in 72.5 per cent versus 25.4 per cent (corrected P values each less than 0.0007). DRw2 was not found in any of the patients but was present in 26.9 per cent of unaffected persons (P corrected less than 0.035). There is thus a negative correlation between this specificity and juvenile-onset diabetes. By contrast, no meaningful differences were found in the frequencies of A, B, or C locus antigens. Studies in white persons with juvenile-onset diabetes have suggested that the reported HLA-B associations are due to HLA-D region specificities, and our results also support the premise that D region specificities are the primary associations with juvenile-onset diabetes."}
{"id": "PMID:481513", "title": "Competition in the delivery of medical care.", "content": "One approach to reform of the medical-care-delivery system emphasizes the development of constructive competition among providers of health care. In this article we describe competition among providers in Minneapolis-St. Paul, one of the few areas that can provide information concerning the practicality of this type of reform. We have found that competition has helped to reduce hospitalization, contain costs and improve access to medical services. At the same time it has focused attention on consumer satisfaction with medical services, increased the range of consumer choice and given consumers better information about providers. Certain public and private measures could facilitate the development of similar competition in other communities.", "contents": "Competition in the delivery of medical care. One approach to reform of the medical-care-delivery system emphasizes the development of constructive competition among providers of health care. In this article we describe competition among providers in Minneapolis-St. Paul, one of the few areas that can provide information concerning the practicality of this type of reform. We have found that competition has helped to reduce hospitalization, contain costs and improve access to medical services. At the same time it has focused attention on consumer satisfaction with medical services, increased the range of consumer choice and given consumers better information about providers. Certain public and private measures could facilitate the development of similar competition in other communities."}
{"id": "PMID:481526", "title": "A neutrophil disorder induced by capnocytophaga, a dental micro-organism.", "content": "We recovered capnocytophaga, a gram-negative anaerobe implicated in the pathogenesis of periodontal disease, from two patients with a history of dental infections. Neutrophils from both patients failed to acquire the asymmetric shape characteristic of normal neutrophils. Fluorescein staining of the patients' living neutrophils remained diffuse and patchy instead of showing the normal pattern in which the fluorescence is swept into the rear of the cell. The locomotion of one patient's neutrophils in vitro was less than 50 per cent of that of normal neutrophils, and migration of this patient's neutrophils into dermal abrasions was reduced, although phagocytosis and nitroblue tetrazolium reduction were normal. All abnormalities of neutrophil morphology and function disappeared after eradication of the capnocytophaga infections. Sonicates and culture medium of capnocytophaga contained a dialyzable substance that caused normal neutrophils to behave like neutrophils obtained from the infected patients.", "contents": "A neutrophil disorder induced by capnocytophaga, a dental micro-organism. We recovered capnocytophaga, a gram-negative anaerobe implicated in the pathogenesis of periodontal disease, from two patients with a history of dental infections. Neutrophils from both patients failed to acquire the asymmetric shape characteristic of normal neutrophils. Fluorescein staining of the patients' living neutrophils remained diffuse and patchy instead of showing the normal pattern in which the fluorescence is swept into the rear of the cell. The locomotion of one patient's neutrophils in vitro was less than 50 per cent of that of normal neutrophils, and migration of this patient's neutrophils into dermal abrasions was reduced, although phagocytosis and nitroblue tetrazolium reduction were normal. All abnormalities of neutrophil morphology and function disappeared after eradication of the capnocytophaga infections. Sonicates and culture medium of capnocytophaga contained a dialyzable substance that caused normal neutrophils to behave like neutrophils obtained from the infected patients."}
{"id": "PMID:481527", "title": "The infant of the diabetic mother: correlation of increased cord C-peptide levels with macrosomia and hypoglycemia.", "content": "C peptide is secreted by pancreatic beta cells in amounts equimolar with insulin, and its levels provide a direct indication of endogenous fetal levels of insulin despite the presence of maternal insulin antibodies. To determine the presence of hyperinsulinemia and its relation to the development of complications in infants of diabetic mothers, we measured cord serum levels of C peptide in 79 infants of diabetic mothers and 62 infants of nondiabetic mothers. Infants of diabetic mothers had higher cord levels of C peptide, which were significantly associated with neonatal hypoglycemia and macrosomia (P less than 0.001) but not with hyaline-membrane disease. Cord levels of C peptide in infants of diabetic mothers were elevated at the earliest gestational age studied (less than 34 weeks) and were directly related to the severity of maternal diabetes, as assessed by the White classification. We conclude that hyperinsulinemia is present in infants of diabetic mothers and that it is related to some major complications in such infants.", "contents": "The infant of the diabetic mother: correlation of increased cord C-peptide levels with macrosomia and hypoglycemia. C peptide is secreted by pancreatic beta cells in amounts equimolar with insulin, and its levels provide a direct indication of endogenous fetal levels of insulin despite the presence of maternal insulin antibodies. To determine the presence of hyperinsulinemia and its relation to the development of complications in infants of diabetic mothers, we measured cord serum levels of C peptide in 79 infants of diabetic mothers and 62 infants of nondiabetic mothers. Infants of diabetic mothers had higher cord levels of C peptide, which were significantly associated with neonatal hypoglycemia and macrosomia (P less than 0.001) but not with hyaline-membrane disease. Cord levels of C peptide in infants of diabetic mothers were elevated at the earliest gestational age studied (less than 34 weeks) and were directly related to the severity of maternal diabetes, as assessed by the White classification. We conclude that hyperinsulinemia is present in infants of diabetic mothers and that it is related to some major complications in such infants."}
{"id": "PMID:481528", "title": "The Ph.D. to M.D. program: the seven-year mark.", "content": "The Ph.D. to M.D. Program, a two-year, accelerated medical curriculum for Ph.D. scientists, has been in operation at the University of Miami for over seven years. Two hundred and nine students from a broad range of scientific disciplines have been admitted, and attrition has been remarkably low (less than 1 per cent). One hundred and thirty-five of the entering candidates (65 per cent) were nonbiologists, but their achievements in the curriculum were virtually equivalent to those of their biologically oriented peers. All graduates have commenced house-staff training, the vast majority at university or university-affiliated hospitals. Of the 46 graduates who have completed some aspect of postgraduate training, 22 are in private practice, 20 are in teaching and research positions and four are scientific administrators in industry. The record indicates that through intensive medical education, the program is able to bring a group of scientists with unusual talents to the theory and practice of medicine.", "contents": "The Ph.D. to M.D. program: the seven-year mark. The Ph.D. to M.D. Program, a two-year, accelerated medical curriculum for Ph.D. scientists, has been in operation at the University of Miami for over seven years. Two hundred and nine students from a broad range of scientific disciplines have been admitted, and attrition has been remarkably low (less than 1 per cent). One hundred and thirty-five of the entering candidates (65 per cent) were nonbiologists, but their achievements in the curriculum were virtually equivalent to those of their biologically oriented peers. All graduates have commenced house-staff training, the vast majority at university or university-affiliated hospitals. Of the 46 graduates who have completed some aspect of postgraduate training, 22 are in private practice, 20 are in teaching and research positions and four are scientific administrators in industry. The record indicates that through intensive medical education, the program is able to bring a group of scientists with unusual talents to the theory and practice of medicine."}
{"id": "PMID:481537", "title": "Protection of granulocytes by vitamin E in glutathione synthetase deficiency.", "content": "The administration of vitamin E (alpha-tocopherol) was found to improve polymorphonuclear leukocyte function in an infant with congenital deficiency of glutathione synthetase activity. Before therapy with vitamin E the abnormal leukocytes exposed to phagocytic challenge showed oxidant damage. They released 60 per cent more hydrogen peroxide than did normal leukocytes, iodinated only 20 to 25 per cent of the normal number of particles, and were unable to kill bacteria as effectively as normal leukocytes although the rates of phagocytosis were normal. These functional abnormalities disappeared when the patient was placed on 400 IU of alpha-tocopherol daily for three months. Associated with the functional improvement was a normalization of microtubule assembly during phagocytic challenge.", "contents": "Protection of granulocytes by vitamin E in glutathione synthetase deficiency. The administration of vitamin E (alpha-tocopherol) was found to improve polymorphonuclear leukocyte function in an infant with congenital deficiency of glutathione synthetase activity. Before therapy with vitamin E the abnormal leukocytes exposed to phagocytic challenge showed oxidant damage. They released 60 per cent more hydrogen peroxide than did normal leukocytes, iodinated only 20 to 25 per cent of the normal number of particles, and were unable to kill bacteria as effectively as normal leukocytes although the rates of phagocytosis were normal. These functional abnormalities disappeared when the patient was placed on 400 IU of alpha-tocopherol daily for three months. Associated with the functional improvement was a normalization of microtubule assembly during phagocytic challenge."}
{"id": "PMID:481557", "title": "Natural occurrence of the mycotoxin penitrem A in moldy cream cheese.", "content": "The occurrence of the mycotoxin penitrem A in refrigerated cream cheese that was involved in the intoxication of two dogs is described. Penitrem A and the fungus Penicillium crustosum were isolated from the cream cheese and identified. This is believed to be the first definitive case of the natural occurrence of penitrem A.", "contents": "Natural occurrence of the mycotoxin penitrem A in moldy cream cheese. The occurrence of the mycotoxin penitrem A in refrigerated cream cheese that was involved in the intoxication of two dogs is described. Penitrem A and the fungus Penicillium crustosum were isolated from the cream cheese and identified. This is believed to be the first definitive case of the natural occurrence of penitrem A."}
{"id": "PMID:481560", "title": "Toxic aspergilli from pistachio nuts.", "content": "Pistachio nut samples taken during various stages of development from orchards in Iran, showed that contamination with fungi occurred mainly during the later stages of nut development. Members of the genera Aspergillus and Penicillium occurred most frequently. Of the Aspergilli, the species A. niger, A. flavus and A. fischeri var. spinosus occurred most frequently, followed by A. terreus, A. tamarii and A. nidulans. Twenty-two isolates comprising 13 species were tested for toxicity to ducklings. Isolates of known toxic fungi included A. flavus, A. niger, A. parasiticus, A. ochraceus, A. versicolor, A. nidulans and A. terreus. The toxicity of A. fischeri var. spinosus is reported. Chemical analysis showed that all isolates of A. flavus and A. parasiticus produced aflatoxin B1, the isolates of A. versicolor and A. nidulans produced sterigmatocystin while the toxic isolate of A. ochraceus did not produce ochratoxins.", "contents": "Toxic aspergilli from pistachio nuts. Pistachio nut samples taken during various stages of development from orchards in Iran, showed that contamination with fungi occurred mainly during the later stages of nut development. Members of the genera Aspergillus and Penicillium occurred most frequently. Of the Aspergilli, the species A. niger, A. flavus and A. fischeri var. spinosus occurred most frequently, followed by A. terreus, A. tamarii and A. nidulans. Twenty-two isolates comprising 13 species were tested for toxicity to ducklings. Isolates of known toxic fungi included A. flavus, A. niger, A. parasiticus, A. ochraceus, A. versicolor, A. nidulans and A. terreus. The toxicity of A. fischeri var. spinosus is reported. Chemical analysis showed that all isolates of A. flavus and A. parasiticus produced aflatoxin B1, the isolates of A. versicolor and A. nidulans produced sterigmatocystin while the toxic isolate of A. ochraceus did not produce ochratoxins."}
{"id": "PMID:481561", "title": "Experimental paracoccidioidomycosis in the Syrian hamster: morphology, ultrastructure and correlation of lesions with presence of specific antigens and serum levels of antibodies.", "content": "Male hamsters (134) received intratesticular injection of a live cerebriform culture of Paracoccidioides brasiliensis and were sacrificed from 6 hours up to 123 days onwards. Tissues from testis, lymph nodes, lungs, liver, spleen, kidneys and intestines were examined microscopically; presence of specific antigens was saught in lesions of testis, regional lymph nodes and liver by indirect immunofluorescence (IF); inoculation site lesions were studied electron microscopically and circulating specific antibodies measured by complement fixation and IF tests. Up to 24 hours inoculation site lesions showed fungi surrounded by PMNs; 48 hours latter macrophages accumulated forming loose nodules; epithelioid granulomata appeared after 5 days. Fungi, scarce in early lesions, increased in numbers up to the time when epithelioid granulomata dominated the picture; in young granulomata fungi were abundant and small; older granulomata contained rare, vacuolated fungi. Ultrastructurally the space between fungi and host-cells was larger around reproducing forms decreasing in size as the parasites grew larger and being a virtual slit around old degenerated fungi. Immunofluorescence studies revealed that fungal walls were brightly fluorescent; in early lesions macrophages surrounding fungi or free in the intersticium contained fluorescent antigenic material in the cytoplasm; similar macrophages were observed in draining lymph nodes as early as 18 hours after inoculation, and latter, in macrophage nodules and Kupffer cells in the liver; epithelioid and giant cells appear to block diffusion of antigens, since in epithelioid granulomata fluorescence was limited to fungal walls. Disseminated paracoccidioidomycosis occurred in 100% of animals after day 5 of infection. Besides specific lesions (containing fungi), antigens were identified by immunofluorescence in 'non specific' lesions in the liver (diffuse or nodular Kupffer cell hyperplasia) and in the lymph nodes (histiocytic hyperplasia). Serum antibodies appeared in low titers, up to day 20, increasing onwards. From day 70 on, titers decreased and lesions changed from confluent epithelioid to loose granulomata infiltrated by PMNs; fungi that before were large and quiescent now were small and in active reproduction. Secondary amyloidosis was present in 85% of the amimals. In the hamster, Paracoccidioidomycosis develops as a chronic progressive disease and the lesions are related both to fungi and its antigens.", "contents": "Experimental paracoccidioidomycosis in the Syrian hamster: morphology, ultrastructure and correlation of lesions with presence of specific antigens and serum levels of antibodies. Male hamsters (134) received intratesticular injection of a live cerebriform culture of Paracoccidioides brasiliensis and were sacrificed from 6 hours up to 123 days onwards. Tissues from testis, lymph nodes, lungs, liver, spleen, kidneys and intestines were examined microscopically; presence of specific antigens was saught in lesions of testis, regional lymph nodes and liver by indirect immunofluorescence (IF); inoculation site lesions were studied electron microscopically and circulating specific antibodies measured by complement fixation and IF tests. Up to 24 hours inoculation site lesions showed fungi surrounded by PMNs; 48 hours latter macrophages accumulated forming loose nodules; epithelioid granulomata appeared after 5 days. Fungi, scarce in early lesions, increased in numbers up to the time when epithelioid granulomata dominated the picture; in young granulomata fungi were abundant and small; older granulomata contained rare, vacuolated fungi. Ultrastructurally the space between fungi and host-cells was larger around reproducing forms decreasing in size as the parasites grew larger and being a virtual slit around old degenerated fungi. Immunofluorescence studies revealed that fungal walls were brightly fluorescent; in early lesions macrophages surrounding fungi or free in the intersticium contained fluorescent antigenic material in the cytoplasm; similar macrophages were observed in draining lymph nodes as early as 18 hours after inoculation, and latter, in macrophage nodules and Kupffer cells in the liver; epithelioid and giant cells appear to block diffusion of antigens, since in epithelioid granulomata fluorescence was limited to fungal walls. Disseminated paracoccidioidomycosis occurred in 100% of animals after day 5 of infection. Besides specific lesions (containing fungi), antigens were identified by immunofluorescence in 'non specific' lesions in the liver (diffuse or nodular Kupffer cell hyperplasia) and in the lymph nodes (histiocytic hyperplasia). Serum antibodies appeared in low titers, up to day 20, increasing onwards. From day 70 on, titers decreased and lesions changed from confluent epithelioid to loose granulomata infiltrated by PMNs; fungi that before were large and quiescent now were small and in active reproduction. Secondary amyloidosis was present in 85% of the amimals. In the hamster, Paracoccidioidomycosis develops as a chronic progressive disease and the lesions are related both to fungi and its antigens."}
{"id": "PMID:481562", "title": "Dermatophyte species, microscopic and cultural examination.", "content": "The dermatophytes Epidermophyton floccosum, Microsporum canis, Trichophyton mentagrophytes var. interdigitale, T. rubrum, and T. verrucosum were compared with respect to the direct microscopic examination of a clinical material and the number of colonies obtained by culture. It was found that the results of microscopy as well as of culture depended to a marked extent upon which species were the cause of the mycosis. The extremes were E. floccosum and T. mentagrophytes var. interdigitale which showed 7.5% and 32.5% isolates with negative microscopic findings and 45.5% and 5.0% isolates with larger than or equal to 10 colonies.", "contents": "Dermatophyte species, microscopic and cultural examination. The dermatophytes Epidermophyton floccosum, Microsporum canis, Trichophyton mentagrophytes var. interdigitale, T. rubrum, and T. verrucosum were compared with respect to the direct microscopic examination of a clinical material and the number of colonies obtained by culture. It was found that the results of microscopy as well as of culture depended to a marked extent upon which species were the cause of the mycosis. The extremes were E. floccosum and T. mentagrophytes var. interdigitale which showed 7.5% and 32.5% isolates with negative microscopic findings and 45.5% and 5.0% isolates with larger than or equal to 10 colonies."}
{"id": "PMID:481564", "title": "Experimental infection of guinea-pigs with atypical and dysgonic strains of microsporum canis.", "content": "Pathogenicity tests with one dysgonic and six atypical strains of Microsporum canis were carried out on guinea-pigs. Five of the atypical strains were laboratory mutants from dysgonic strains isolated from living hosts. As sporulation and viability varied greatly between the strains, inocula consisted of suspensions of fungal fragments of known viable count. When a sufficiently active inoculum was used, lesions and fluorescent hairs were induced in the guinea-pigs by all but one of the strains tested. In each case the strain inoculated was reisolated from the lesions in pure culture. The significance of the results is discussed in the light of the unusual nature and origin of the strains.", "contents": "Experimental infection of guinea-pigs with atypical and dysgonic strains of microsporum canis. Pathogenicity tests with one dysgonic and six atypical strains of Microsporum canis were carried out on guinea-pigs. Five of the atypical strains were laboratory mutants from dysgonic strains isolated from living hosts. As sporulation and viability varied greatly between the strains, inocula consisted of suspensions of fungal fragments of known viable count. When a sufficiently active inoculum was used, lesions and fluorescent hairs were induced in the guinea-pigs by all but one of the strains tested. In each case the strain inoculated was reisolated from the lesions in pure culture. The significance of the results is discussed in the light of the unusual nature and origin of the strains."}
{"id": "PMID:481565", "title": "[Substrate and parasubstrate regulation of physiological processes at various levels of the organization of living systems as illustrated by examples of the regulation of appetite, of the specific dynamic action of food and of the inhibition of processes of absorption].", "content": "It was demonstrated that in the organism of higher animals the adaptation regulation of food uptake and nutrient absorption and transport comprises, in addition to the classic mechanisms of substrate regulation, specialized non-substrate and parasubstrate mechanisms which control the uptake of food and other substrates at all three levels (organosystemic, organic and cellular). As to the organosystemic level, it was observed in duodenectomized rats that appetite inhibition is produced not only by a stimulation of the receptors and by an increase in the concentration of the food substrates and of the metabolites in the blood, but also by one of the intestinal appetite-inhibiting hormones, arenterin. As to the organic level, it was evidenced that the enzymatic and transport functions of the small intestine and the numerical composition of the enterocyte population in the different segments are determined by the substrate load on the respective areas of the small intestine as well as by the blood and chyme-mediated intersegmental reactions in the small intestine. As to the tissular and cellular levels, the possibility of a regulation of the enzymatic and transport systems of the microvilli by means of substrates contained in the mucous membrane of the small intestine and endogenous substances (permein and antipermein) was evinced.", "contents": "[Substrate and parasubstrate regulation of physiological processes at various levels of the organization of living systems as illustrated by examples of the regulation of appetite, of the specific dynamic action of food and of the inhibition of processes of absorption]. It was demonstrated that in the organism of higher animals the adaptation regulation of food uptake and nutrient absorption and transport comprises, in addition to the classic mechanisms of substrate regulation, specialized non-substrate and parasubstrate mechanisms which control the uptake of food and other substrates at all three levels (organosystemic, organic and cellular). As to the organosystemic level, it was observed in duodenectomized rats that appetite inhibition is produced not only by a stimulation of the receptors and by an increase in the concentration of the food substrates and of the metabolites in the blood, but also by one of the intestinal appetite-inhibiting hormones, arenterin. As to the organic level, it was evidenced that the enzymatic and transport functions of the small intestine and the numerical composition of the enterocyte population in the different segments are determined by the substrate load on the respective areas of the small intestine as well as by the blood and chyme-mediated intersegmental reactions in the small intestine. As to the tissular and cellular levels, the possibility of a regulation of the enzymatic and transport systems of the microvilli by means of substrates contained in the mucous membrane of the small intestine and endogenous substances (permein and antipermein) was evinced."}
{"id": "PMID:481566", "title": "[Regulatory properties of the intestinal enzymes of higher and lower animals as an adaptation mechanism in digestion and absorption].", "content": "It is shown that certain key enzymes in membranous digestion (alkaline phosphatase, peptidase, gamma-amylase) are allosteric and ensure the autoregulation and the homoeostasis of the final stages of hydrolysis and of the initial stages of nutrient transport. This mechanism was evidenced not only in vertebrates (mammals, birds, fishes), but also in invertebrates (drosophilae). The comparison of the triton and trypsin forms of the enzymes permitted to locate centres of regulation in the hydrophobic parts of amphipathetic enzymes (as illustrated by the examples of alkaline phosphatase and gamma-amylase of of the rat and of the drosophila). A considerable variability of the regulatory characteristics of the enzymes under investigation was demonstrated in the different varieties of drosophila. The authors present a hypothesis on the role of the regulatory properties of digestive enzymes in the physiology and the pathology of the digestive and transport systems of the small intestine.", "contents": "[Regulatory properties of the intestinal enzymes of higher and lower animals as an adaptation mechanism in digestion and absorption]. It is shown that certain key enzymes in membranous digestion (alkaline phosphatase, peptidase, gamma-amylase) are allosteric and ensure the autoregulation and the homoeostasis of the final stages of hydrolysis and of the initial stages of nutrient transport. This mechanism was evidenced not only in vertebrates (mammals, birds, fishes), but also in invertebrates (drosophilae). The comparison of the triton and trypsin forms of the enzymes permitted to locate centres of regulation in the hydrophobic parts of amphipathetic enzymes (as illustrated by the examples of alkaline phosphatase and gamma-amylase of of the rat and of the drosophila). A considerable variability of the regulatory characteristics of the enzymes under investigation was demonstrated in the different varieties of drosophila. The authors present a hypothesis on the role of the regulatory properties of digestive enzymes in the physiology and the pathology of the digestive and transport systems of the small intestine."}
{"id": "PMID:481567", "title": "[Use of dichlorvos in flour mills].", "content": "In the framework of studies on the possible use of dichlorvos (DDVP) in flour mills, the authors investigated the effects of various parameters (such as concentration of the DDVP solution to be used, temperature, location of the samples, different kinds of samples and packages) on the formation of DDVP residues on milling products. It was found that a concentration of 0.6 ml of DDVP-50/m3 and temperatures ranging from 18 degrees to 22 degrees C are particularly suited for insect control. Under these conditions, the DDVP residues on the samples were degraded within a period of 2--4 weeks, according to the kind of product. Uncovered foods shall not be present in rooms where DDVP is being applied. In virtue of the present findings, DDVP is recommended for use in flour mills.", "contents": "[Use of dichlorvos in flour mills]. In the framework of studies on the possible use of dichlorvos (DDVP) in flour mills, the authors investigated the effects of various parameters (such as concentration of the DDVP solution to be used, temperature, location of the samples, different kinds of samples and packages) on the formation of DDVP residues on milling products. It was found that a concentration of 0.6 ml of DDVP-50/m3 and temperatures ranging from 18 degrees to 22 degrees C are particularly suited for insect control. Under these conditions, the DDVP residues on the samples were degraded within a period of 2--4 weeks, according to the kind of product. Uncovered foods shall not be present in rooms where DDVP is being applied. In virtue of the present findings, DDVP is recommended for use in flour mills."}
{"id": "PMID:481568", "title": "[Adaption of protein in infant feeding].", "content": "A regarding its intestinal microecological action like human milk performed infant formula with a protein content of 1,6%, exclusively as casein, caused in nature born infants the same retention of nitrogen as human milk did. In prematures addition of cystine led to an increased retention. The serum level of urea and the pH of the feces were enhanced up to an undesirable degree when an analogous formula with 2,3% casein was applicated.", "contents": "[Adaption of protein in infant feeding]. A regarding its intestinal microecological action like human milk performed infant formula with a protein content of 1,6%, exclusively as casein, caused in nature born infants the same retention of nitrogen as human milk did. In prematures addition of cystine led to an increased retention. The serum level of urea and the pH of the feces were enhanced up to an undesirable degree when an analogous formula with 2,3% casein was applicated."}
{"id": "PMID:481569", "title": "[Alteration of the normal intestinal flora in human sucklings within the last 20 years].", "content": "Analyses of the feces-flora and the feces-pH of breastfed infants and of infants nourished with commercial formulae (Ki-Na, Milasan) performed since 20 years were evaluated. In both regimens decreasing numbers of Bifidobacteria could be remarked. The dominating position of this groups of bacteria was limited more and more. Since 1971 the Bifidobacterium biotype, characteristic of feces of breastfed infants could be proved no more. Simultaneously germs of the E. coli-group and neonmycine-resistent forms increased. With breast-milk Proteus distinctly increased in the feces, whereas, formulae on cow's milk basis caused the reversed effect. Bacteroides and the group of H2S producing bacteria remained unaltered. In the stools of breastfed infants the pH-value increased slowly. These tendencies were marked still stronger in prematures. Enhancing influences from the environment as possible reasons for these developments were discussed.", "contents": "[Alteration of the normal intestinal flora in human sucklings within the last 20 years]. Analyses of the feces-flora and the feces-pH of breastfed infants and of infants nourished with commercial formulae (Ki-Na, Milasan) performed since 20 years were evaluated. In both regimens decreasing numbers of Bifidobacteria could be remarked. The dominating position of this groups of bacteria was limited more and more. Since 1971 the Bifidobacterium biotype, characteristic of feces of breastfed infants could be proved no more. Simultaneously germs of the E. coli-group and neonmycine-resistent forms increased. With breast-milk Proteus distinctly increased in the feces, whereas, formulae on cow's milk basis caused the reversed effect. Bacteroides and the group of H2S producing bacteria remained unaltered. In the stools of breastfed infants the pH-value increased slowly. These tendencies were marked still stronger in prematures. Enhancing influences from the environment as possible reasons for these developments were discussed."}
{"id": "PMID:481571", "title": "Nonhormonal perinatal carcinogenesis.", "content": "General concepts of chemical carcinogenesis are reviewed and implications of these concepts in perinatal carcinogenesis, as well as interrelationships between embryonal mortality, teratogenicity, and carcinogenicity are discussed.", "contents": "Nonhormonal perinatal carcinogenesis. General concepts of chemical carcinogenesis are reviewed and implications of these concepts in perinatal carcinogenesis, as well as interrelationships between embryonal mortality, teratogenicity, and carcinogenicity are discussed."}
{"id": "PMID:481572", "title": "Teratogenic and carcinogenic effects of some chemicals during perinatal life in rats, Syrian golden hamsters, and minipigs.", "content": "Teratogenic effects of ENU have been observed in the rat, Syrian golden hamster, and minipig. In BD and Wistar rats, as well as in hamsters, ENU is a potent carcinogen when administered prenatally. Other members of the homologous series of alkylnitrosoureas, except n-propylnitrosourea, have been shown to be less active or totally inactive as carcinogens in experiments on prenatal animals. Simultaneous oral administration of L-citrulline and sodium nitrite induced adenosarcomas of the kidney (Wilm's tumors) in 6 of 22 offspring. The importance of prophylactic measures in man during prenatal development is emphasized.", "contents": "Teratogenic and carcinogenic effects of some chemicals during perinatal life in rats, Syrian golden hamsters, and minipigs. Teratogenic effects of ENU have been observed in the rat, Syrian golden hamster, and minipig. In BD and Wistar rats, as well as in hamsters, ENU is a potent carcinogen when administered prenatally. Other members of the homologous series of alkylnitrosoureas, except n-propylnitrosourea, have been shown to be less active or totally inactive as carcinogens in experiments on prenatal animals. Simultaneous oral administration of L-citrulline and sodium nitrite induced adenosarcomas of the kidney (Wilm's tumors) in 6 of 22 offspring. The importance of prophylactic measures in man during prenatal development is emphasized."}
{"id": "PMID:481573", "title": "Tissue differentiation as a prerequisite for transplacental carcinogenesis in the hamster respiratory system, with specific respect to the trachea.", "content": "The significance of tissue differentiation for the transplacental carcinogenicity of DEN was examined. In one experiment, pregnant Syrian golden hamsters received a single sc injection of DEN on one of the different days of pregnancy. Approximately 95% of the offspring of those mothers treated on one of the last 4 days (days 12--15) of gestation developed respiratory tract tumors. Transplacental DEN treatment before the 12th prenatal day failed to induce any neoplastic response in the young. In the second experiment, the differentiation of the prenatal Syrian golden hamster tracheal epithelium was examined histologically and by electron microscopy. We found that on the 12th prenatal day the ER occurred for the first time in its functionally competent form. On earlier prenatal days, the epithelial cells lacked this organelle. We conclude that this development of ER is a prerequisite for transplacental DEN carcinogenesis, since this organelle contains the nonspecific enzyme systems necessary for the transformation of DEN to its ultimate carcinogen.", "contents": "Tissue differentiation as a prerequisite for transplacental carcinogenesis in the hamster respiratory system, with specific respect to the trachea. The significance of tissue differentiation for the transplacental carcinogenicity of DEN was examined. In one experiment, pregnant Syrian golden hamsters received a single sc injection of DEN on one of the different days of pregnancy. Approximately 95% of the offspring of those mothers treated on one of the last 4 days (days 12--15) of gestation developed respiratory tract tumors. Transplacental DEN treatment before the 12th prenatal day failed to induce any neoplastic response in the young. In the second experiment, the differentiation of the prenatal Syrian golden hamster tracheal epithelium was examined histologically and by electron microscopy. We found that on the 12th prenatal day the ER occurred for the first time in its functionally competent form. On earlier prenatal days, the epithelial cells lacked this organelle. We conclude that this development of ER is a prerequisite for transplacental DEN carcinogenesis, since this organelle contains the nonspecific enzyme systems necessary for the transformation of DEN to its ultimate carcinogen."}
{"id": "PMID:481574", "title": "Host factors affecting perinatal carcinogenesis by resorptive alkylnitrosoureas in rats.", "content": "Host factors are operative in transplacental carcinogenesis. There are species, strain, sex, and individual differences in susceptibility to carcinogens. Rat fetuses are over 50 times more susceptible than adults to the oncogenic effects of ENU, yet are less susceptible to tumor induction with DMN. Immune mechanisms do not seem to affect the latency period, incidence, location, and type of neurogenic neoplasms, although tumor-specific antigens are demonstrable in neoplastic neuroectodermal cells. Although placental barriers, DNA repair capacities, age at exposure, hormonal interaction, and immune responsiveness may be singled out as possible causes for species, strain, sex, and individual variations in susceptibility to the neuro-oncogenic potential of ENU, no definitive proof of the mechanism of action of any of these factors has yet been presented.", "contents": "Host factors affecting perinatal carcinogenesis by resorptive alkylnitrosoureas in rats. Host factors are operative in transplacental carcinogenesis. There are species, strain, sex, and individual differences in susceptibility to carcinogens. Rat fetuses are over 50 times more susceptible than adults to the oncogenic effects of ENU, yet are less susceptible to tumor induction with DMN. Immune mechanisms do not seem to affect the latency period, incidence, location, and type of neurogenic neoplasms, although tumor-specific antigens are demonstrable in neoplastic neuroectodermal cells. Although placental barriers, DNA repair capacities, age at exposure, hormonal interaction, and immune responsiveness may be singled out as possible causes for species, strain, sex, and individual variations in susceptibility to the neuro-oncogenic potential of ENU, no definitive proof of the mechanism of action of any of these factors has yet been presented."}
{"id": "PMID:481575", "title": "Mechanism of perinatal tumor induction by neuro-oncogenic alkylnitrosoureas and dialkylaryltriazenes.", "content": "The methylation by DMPT and MNU of DNA from rat liver and brain was investigated at various developmental stages. Following a single sc injection of [14C]DMPT (100 mg/kg body wt, 15 hr survival time) in pregnant rats (21st day of gestation), the extent of methylation of purine bases was similar in fetal liver and brain. During postnatal growth, this treatment resulted in an increasingly preferential methylation of liver DNA. In 30-day-old BD-IX rats, the concentration of 7-methylguanine in liver was approximately eight times higher than in brain DNA. This suggested that during prenatal development, both liver and brain DNA are transplacentally methylated by a proximate carcinogen produced by maternal organs. After a single ip injection of [3H]MNU (10 mg/kg body wt) to 10-day-old rats, O6-methylguanine was more rapidly removed from hepatic than from cerebral DNA. Within 1 week after the injection, the brain-to-liver ratio for 06-methylguanine increased from 1.4 to 98. These results are compatible with the hypothesis that the deficiency of various organs for repair excision of O6-alkylguanine from DNA correlates with their susceptibility to malignant transformation by monofunctional alkylating carcinogens.", "contents": "Mechanism of perinatal tumor induction by neuro-oncogenic alkylnitrosoureas and dialkylaryltriazenes. The methylation by DMPT and MNU of DNA from rat liver and brain was investigated at various developmental stages. Following a single sc injection of [14C]DMPT (100 mg/kg body wt, 15 hr survival time) in pregnant rats (21st day of gestation), the extent of methylation of purine bases was similar in fetal liver and brain. During postnatal growth, this treatment resulted in an increasingly preferential methylation of liver DNA. In 30-day-old BD-IX rats, the concentration of 7-methylguanine in liver was approximately eight times higher than in brain DNA. This suggested that during prenatal development, both liver and brain DNA are transplacentally methylated by a proximate carcinogen produced by maternal organs. After a single ip injection of [3H]MNU (10 mg/kg body wt) to 10-day-old rats, O6-methylguanine was more rapidly removed from hepatic than from cerebral DNA. Within 1 week after the injection, the brain-to-liver ratio for 06-methylguanine increased from 1.4 to 98. These results are compatible with the hypothesis that the deficiency of various organs for repair excision of O6-alkylguanine from DNA correlates with their susceptibility to malignant transformation by monofunctional alkylating carcinogens."}
{"id": "PMID:481576", "title": "Perinatal carcinogenesis: biologic curiosity or practical necessity?", "content": "Factors that require consideration in extending carcinogen bioassay protocols to include transplacental exposure of rodent subjects are summarized. These include metabolic complexities and biologic problems, such as the differences in fetal susceptibility to lethal, teratogenic, and carcinogenic effects of the same compound at different stages of intrauterine development, and that, with the present limited knowledge of transplacental carcinogenesis, transplacental and neonatal exposure to suspected carcinogens may lead to insoluble problems of interpretation. The view is expressed that agents to which the human fetus may be substantially exposed, including drugs and food additives, should nevertheless be tested for carcinogenicity by transplacental exposure.", "contents": "Perinatal carcinogenesis: biologic curiosity or practical necessity? Factors that require consideration in extending carcinogen bioassay protocols to include transplacental exposure of rodent subjects are summarized. These include metabolic complexities and biologic problems, such as the differences in fetal susceptibility to lethal, teratogenic, and carcinogenic effects of the same compound at different stages of intrauterine development, and that, with the present limited knowledge of transplacental carcinogenesis, transplacental and neonatal exposure to suspected carcinogens may lead to insoluble problems of interpretation. The view is expressed that agents to which the human fetus may be substantially exposed, including drugs and food additives, should nevertheless be tested for carcinogenicity by transplacental exposure."}
{"id": "PMID:481577", "title": "Prenatal diethylstilbestrol exposure and human genital tract abnormalities.", "content": "The incidence of clear cell adenocarcinoma of the vagina and cervix associated with intrauterine exposure to DES and similar compounds during the first half of pregnancy has increased. Ninety percent of these cancers have occurred in patients 14 years of age or older. Although these carcinomas are exceedingly rare, nonneoplastic abnormalities including vaginal adenosis, cervical eversion (ectropion), and transverse cervical and vaginal ridges are frequent in the exposed population, particularly if the drug was administered early in pregnancy. Current evidence favors a disturbance in development of the m\u00fcllerian duct as the explanation of these changes. Whether DES is only a teratogen or also a carcinogen is unknown, as is the possible role of other factors in the development of cancer. An increased incidence of cancer among exposed males has not been documented.", "contents": "Prenatal diethylstilbestrol exposure and human genital tract abnormalities. The incidence of clear cell adenocarcinoma of the vagina and cervix associated with intrauterine exposure to DES and similar compounds during the first half of pregnancy has increased. Ninety percent of these cancers have occurred in patients 14 years of age or older. Although these carcinomas are exceedingly rare, nonneoplastic abnormalities including vaginal adenosis, cervical eversion (ectropion), and transverse cervical and vaginal ridges are frequent in the exposed population, particularly if the drug was administered early in pregnancy. Current evidence favors a disturbance in development of the m\u00fcllerian duct as the explanation of these changes. Whether DES is only a teratogen or also a carcinogen is unknown, as is the possible role of other factors in the development of cancer. An increased incidence of cancer among exposed males has not been documented."}
{"id": "PMID:481578", "title": "In vivo studies in Syrian golden hamsters: a transplacental bioassay of ten nitrosamines.", "content": "The carcinogenic effects of low doses of 10 nitrosamines were determined in pregnant Syrian golden hamsters and their offspring. Compounds studied included dimethylnitrosamine, di-n-propylnitrosamine, di-n-butylnitrosamine, nitrosopiperidine, nitrosohexamethyleneimine, 2-dydroxypropyl-propyl-nitrosamine, 2-oxopropyl-propyl-nitrosamine, methylpropylnitrosamine, di(2-hydroxypropyl) nitrosamine, and 4-hydroxybutyl-butyl-nitrosamine. Tumor incidences of all organ systems were almost always higher and latencies shorter in the mothers than in the offspring. Exceptions occurred in the respiratory system in which several compounds induced a low incidence of tumors in the offspring but none in the mothers. Fetal susceptibility appeared greatest toward the end of gestation. For purposes of bioassay, transplacental exposure was less efficient than conventional adult treatment.", "contents": "In vivo studies in Syrian golden hamsters: a transplacental bioassay of ten nitrosamines. The carcinogenic effects of low doses of 10 nitrosamines were determined in pregnant Syrian golden hamsters and their offspring. Compounds studied included dimethylnitrosamine, di-n-propylnitrosamine, di-n-butylnitrosamine, nitrosopiperidine, nitrosohexamethyleneimine, 2-dydroxypropyl-propyl-nitrosamine, 2-oxopropyl-propyl-nitrosamine, methylpropylnitrosamine, di(2-hydroxypropyl) nitrosamine, and 4-hydroxybutyl-butyl-nitrosamine. Tumor incidences of all organ systems were almost always higher and latencies shorter in the mothers than in the offspring. Exceptions occurred in the respiratory system in which several compounds induced a low incidence of tumors in the offspring but none in the mothers. Fetal susceptibility appeared greatest toward the end of gestation. For purposes of bioassay, transplacental exposure was less efficient than conventional adult treatment."}
{"id": "PMID:481579", "title": "Rapid screening for chemical carcinogens: transforming activity of selected nitroso compounds detected in a transplacental host-mediated culture system.", "content": "The transplacental host-mediated hamster cell culture system was used to test a variety of solvents and chemicals of unknown and known (positive and negative) activity for their ability to induce morphologic transformation of cells and growth in agar. Examination of approximately 13,000 colonies of cells from untreated animals yielded no transformants, thus demonstrating no spontaneous transformation in the system. Similar negative results were obtained after animals were treated with the solvents acetone, ethanol, dimethyl sulfoxide, dimethylformamide, and trioctanoin oil. Several known carcinogens, including benzo[a]pyrene, methylnitrosourethane, urethan, and diethylnitrosamine, were positive for transforming activity. Three pesticides, carbaryl, methomyl, and landrin, and their N-nitroso derivatives were tested. All the nitrosated forms had transforming activity, but only one of the pesticides, landrin, was positive. In all experiments conducted, results of the agar-growth test correlated well with tests for morphologic transformation. The transplacental hamster embryol cell culture system therefore detected transforming activity of N-nitroso compounds and some known carcinogens.", "contents": "Rapid screening for chemical carcinogens: transforming activity of selected nitroso compounds detected in a transplacental host-mediated culture system. The transplacental host-mediated hamster cell culture system was used to test a variety of solvents and chemicals of unknown and known (positive and negative) activity for their ability to induce morphologic transformation of cells and growth in agar. Examination of approximately 13,000 colonies of cells from untreated animals yielded no transformants, thus demonstrating no spontaneous transformation in the system. Similar negative results were obtained after animals were treated with the solvents acetone, ethanol, dimethyl sulfoxide, dimethylformamide, and trioctanoin oil. Several known carcinogens, including benzo[a]pyrene, methylnitrosourethane, urethan, and diethylnitrosamine, were positive for transforming activity. Three pesticides, carbaryl, methomyl, and landrin, and their N-nitroso derivatives were tested. All the nitrosated forms had transforming activity, but only one of the pesticides, landrin, was positive. In all experiments conducted, results of the agar-growth test correlated well with tests for morphologic transformation. The transplacental hamster embryol cell culture system therefore detected transforming activity of N-nitroso compounds and some known carcinogens."}
{"id": "PMID:481580", "title": "Incorporation of transplacental exposure into routine carcinogenicity bioassays.", "content": "Theoretical and practical issues related to incorporating transplacental and neonatal exposure into routine toxicology assays for carcinogen detection are presented. In view of the many chemicals that have not been evaluated for carcinogenic potential, an integrated approach to carcinogenesis bioassay is suggested in which short-term tests to predict potential carcinogens and a combined transplacental-neonatal-conventional exposure protocol for animal studies are used.", "contents": "Incorporation of transplacental exposure into routine carcinogenicity bioassays. Theoretical and practical issues related to incorporating transplacental and neonatal exposure into routine toxicology assays for carcinogen detection are presented. In view of the many chemicals that have not been evaluated for carcinogenic potential, an integrated approach to carcinogenesis bioassay is suggested in which short-term tests to predict potential carcinogens and a combined transplacental-neonatal-conventional exposure protocol for animal studies are used."}
{"id": "PMID:481581", "title": "Development of permanently proliferated and cornified vaginal epithelium in mice treated neonatally with steroid hormones and the implication in tumorigenesis.", "content": "Five to ten daily injections of 5--20 micrograms 17 beta-estradiol, 20--100 micrograms testosterone and its propionate, and 100 micrograms 5 alpha-dihydrotestosterone starting within 24 hours after bith caused estrogen-independent persistent proliferation and cornification of the vaginal epithelium in female mice of the C57BL/Tw, C57BL/Ms, A/Ms, and A/CrgI strains. The permanently changed vaginas frequently resulted in tumorigenesis. In C57BL/Tw mice, some undifferentiated cells, which survive only for a few postnatal days in normal animals, appeared to transform into large cells and formed nodules after neonatal administration of 17 beta-estradiol. The primary normal epithelium was replaced by a sheet of fused nodules that showed persistent proliferation and cornification independent of estrogen. Occurrence of the estrogen-independent vaginal epithelium in C57BL mice receiving neonatal injections of 20 micrograms estradiol for 5 days was prevented by injections of 200 IU VA when given simultaneously with the estradiol. Light cells were found by electron microscopy in the epithelium of estradiol-plus VA-treated mice. How these cells may have prevented the occurrence of the permanently changed vaginal epithelium is discussed.", "contents": "Development of permanently proliferated and cornified vaginal epithelium in mice treated neonatally with steroid hormones and the implication in tumorigenesis. Five to ten daily injections of 5--20 micrograms 17 beta-estradiol, 20--100 micrograms testosterone and its propionate, and 100 micrograms 5 alpha-dihydrotestosterone starting within 24 hours after bith caused estrogen-independent persistent proliferation and cornification of the vaginal epithelium in female mice of the C57BL/Tw, C57BL/Ms, A/Ms, and A/CrgI strains. The permanently changed vaginas frequently resulted in tumorigenesis. In C57BL/Tw mice, some undifferentiated cells, which survive only for a few postnatal days in normal animals, appeared to transform into large cells and formed nodules after neonatal administration of 17 beta-estradiol. The primary normal epithelium was replaced by a sheet of fused nodules that showed persistent proliferation and cornification independent of estrogen. Occurrence of the estrogen-independent vaginal epithelium in C57BL mice receiving neonatal injections of 20 micrograms estradiol for 5 days was prevented by injections of 200 IU VA when given simultaneously with the estradiol. Light cells were found by electron microscopy in the epithelium of estradiol-plus VA-treated mice. How these cells may have prevented the occurrence of the permanently changed vaginal epithelium is discussed."}
{"id": "PMID:481582", "title": "Transplacental effects of diethylstilbestrol in mice.", "content": "The effect of prenatal exposure to DES on the postnatal development of male and female genital tract function was studied. We investigated the placental transfer of [3H]- or [14C]-radiolabeled DES in pregnant mice. DES-associated radioactivity in the fetal plasma approximated maternal plasma one-half hour after iv administration of [3H]DES; [3H]-acitivity associated with DES in the fetal genital tract was about threefold higher. The decrease in reproductive capacity of female offspring from mice treated with DES during gestation was dose related; a low incidence (10% or less) of cancer of the vagina, cervix, and/or uterus was also observed in these mice. Male offspring exposed prenatally to the highest dose (100 micrograms/kg) of DES in this study also had lower reproductive capacities. Lesions in the genital tract of these mice included epididymal cysts, inflammation, cryptorchidism, and nodular masses in the seminal vesicles and/or prostate gland. Such lesions and sterility were not observed at the lower DES doses. Histologic studies with neonatal mice raise the possibility that m\u00fcllerian duct tissue may represent a site for the transplacental toxicity of DES in both the male and female fetus.", "contents": "Transplacental effects of diethylstilbestrol in mice. The effect of prenatal exposure to DES on the postnatal development of male and female genital tract function was studied. We investigated the placental transfer of [3H]- or [14C]-radiolabeled DES in pregnant mice. DES-associated radioactivity in the fetal plasma approximated maternal plasma one-half hour after iv administration of [3H]DES; [3H]-acitivity associated with DES in the fetal genital tract was about threefold higher. The decrease in reproductive capacity of female offspring from mice treated with DES during gestation was dose related; a low incidence (10% or less) of cancer of the vagina, cervix, and/or uterus was also observed in these mice. Male offspring exposed prenatally to the highest dose (100 micrograms/kg) of DES in this study also had lower reproductive capacities. Lesions in the genital tract of these mice included epididymal cysts, inflammation, cryptorchidism, and nodular masses in the seminal vesicles and/or prostate gland. Such lesions and sterility were not observed at the lower DES doses. Histologic studies with neonatal mice raise the possibility that m\u00fcllerian duct tissue may represent a site for the transplacental toxicity of DES in both the male and female fetus."}
{"id": "PMID:481583", "title": "Diethylstilbestrol: evidence for metabolic activation in man, rat, and hamster.", "content": "Oxidative biotransformation of radioactively and deuterium-labeled DES gives rise to several metabolites in intact Wistar rats, Syrian golden hamsters, and humans. With the use of radio gas chromatography and gas chromatography--mass spectrometry, the major urinary and biliary metabolites were tentatively identified as hydroxy and methoxy derivatives of DES and dienestrol, of which the relative amounts excreted depended largely on the species. Some of the metabolites are potentially reactive substances or have reactive metabolic precursors such as epoxides or allylic hydroxy compounds that might be associated with the adverse effects of DES.", "contents": "Diethylstilbestrol: evidence for metabolic activation in man, rat, and hamster. Oxidative biotransformation of radioactively and deuterium-labeled DES gives rise to several metabolites in intact Wistar rats, Syrian golden hamsters, and humans. With the use of radio gas chromatography and gas chromatography--mass spectrometry, the major urinary and biliary metabolites were tentatively identified as hydroxy and methoxy derivatives of DES and dienestrol, of which the relative amounts excreted depended largely on the species. Some of the metabolites are potentially reactive substances or have reactive metabolic precursors such as epoxides or allylic hydroxy compounds that might be associated with the adverse effects of DES."}
{"id": "PMID:481584", "title": "Role of hormone imbalance in transplacental carcinogenesis induced in Syrian golden hamsters by sex hormones.", "content": "Data are presented from studies on Syrian golden hamsters with the ENU precursors, EU, and NaNO2, given transplacentally and in adulthood, and with transplacentally administered DES. Hormone modification by gonadectomy of offspring prenatally exposed to ENU caused a significantly greater incidence and multiplicity of PNS neoplasms and other tumor types in orchidectomized males, compared with intact males, and in ovariectomized and intact females. That PNS tumors in gonadectomized males appeared within a significantly shorter latency period indicated that endogenously generated androgens inhibited neoplastic development. The endocrine imbalance also induced a higher incidence of neoplasia in other tissues and organs, e.g., skin melanomas, thyroid and adrenal cortex tumors, and notably gliomas in the CNS of ovariectomized female siblings. Exposure to single doses of ENU on days 12, 13, 14, and/or 15 caused PNS tumors predominantly in females and with an increased frequency in progeny treated during the final days of gestation. The spectrum of neoplasms was greater and their incidence significant in ENU-treated adult hamsters; the tumor types different from those of transplacentally treated animals (i.e., vascular, vaginal, and ovarian tumors and fore-stomach papillomas were seen). Determining factors in carcinogenesis at the time of carcinogen treatment possibly included stage of ontogenic development, degree of cell differentiation, hormone state of host, age, total dose, and duration of treatment. DES results indicated that the haster may be a useful model for reproducing lesions similar to those observed in children of mothers treated with this drug during pregnancy.", "contents": "Role of hormone imbalance in transplacental carcinogenesis induced in Syrian golden hamsters by sex hormones. Data are presented from studies on Syrian golden hamsters with the ENU precursors, EU, and NaNO2, given transplacentally and in adulthood, and with transplacentally administered DES. Hormone modification by gonadectomy of offspring prenatally exposed to ENU caused a significantly greater incidence and multiplicity of PNS neoplasms and other tumor types in orchidectomized males, compared with intact males, and in ovariectomized and intact females. That PNS tumors in gonadectomized males appeared within a significantly shorter latency period indicated that endogenously generated androgens inhibited neoplastic development. The endocrine imbalance also induced a higher incidence of neoplasia in other tissues and organs, e.g., skin melanomas, thyroid and adrenal cortex tumors, and notably gliomas in the CNS of ovariectomized female siblings. Exposure to single doses of ENU on days 12, 13, 14, and/or 15 caused PNS tumors predominantly in females and with an increased frequency in progeny treated during the final days of gestation. The spectrum of neoplasms was greater and their incidence significant in ENU-treated adult hamsters; the tumor types different from those of transplacentally treated animals (i.e., vascular, vaginal, and ovarian tumors and fore-stomach papillomas were seen). Determining factors in carcinogenesis at the time of carcinogen treatment possibly included stage of ontogenic development, degree of cell differentiation, hormone state of host, age, total dose, and duration of treatment. DES results indicated that the haster may be a useful model for reproducing lesions similar to those observed in children of mothers treated with this drug during pregnancy."}
{"id": "PMID:481590", "title": "H-2 restriction of contact inhibition of epithelial cells.", "content": "Medawar has suggested that the major histocompatibility gene complex (MHC) might be involved in the contact inhibition of movement shown by fibroblasts and epithelia. Contact inhibition of movement is that reaction of cells which stops the movement of one cell over another and thus leads to monolayering and other features of morphology typical of cells in culture and in vivo. By confronting epithelial outgrowths we have compared contact inhibition between syngeneic cells with that between allogeneic cells. Contact inhibition was more marked between allogeneic combinations than syngeneic combinations, when the genetic mismatch lay in certain parts of the MHC complex.", "contents": "H-2 restriction of contact inhibition of epithelial cells. Medawar has suggested that the major histocompatibility gene complex (MHC) might be involved in the contact inhibition of movement shown by fibroblasts and epithelia. Contact inhibition of movement is that reaction of cells which stops the movement of one cell over another and thus leads to monolayering and other features of morphology typical of cells in culture and in vivo. By confronting epithelial outgrowths we have compared contact inhibition between syngeneic cells with that between allogeneic cells. Contact inhibition was more marked between allogeneic combinations than syngeneic combinations, when the genetic mismatch lay in certain parts of the MHC complex."}
{"id": "PMID:481592", "title": "Macromolecular structural transitions in Pf1 filamentous bacterial virus.", "content": "The filamentous bacterial virus Pf1 is a simple model for biological filaments. We have studied the structure of the virion and report here that the helix parameters of Pf1 change sharply with temperature at about 8 degrees C. Local interactions between protein subunits change by only a few tenths of an angstrom, but the changes are amplified between one end and the other of the virion to a rotation of 15 turns and a translation of 1,000 A. The limited nature of the phase transition is probably due to the constraints of 'knobs-into-holes' interaction between side chains of adjacent alpha-helical protein subunits. Treatment of the virion with ether causes a rearrangement of protein subunits into sheets, with the alpha-helices normal to the plane of the sheet. This phase transition suggests a model for virion assembly in the bacterial membrane.", "contents": "Macromolecular structural transitions in Pf1 filamentous bacterial virus. The filamentous bacterial virus Pf1 is a simple model for biological filaments. We have studied the structure of the virion and report here that the helix parameters of Pf1 change sharply with temperature at about 8 degrees C. Local interactions between protein subunits change by only a few tenths of an angstrom, but the changes are amplified between one end and the other of the virion to a rotation of 15 turns and a translation of 1,000 A. The limited nature of the phase transition is probably due to the constraints of 'knobs-into-holes' interaction between side chains of adjacent alpha-helical protein subunits. Treatment of the virion with ether causes a rearrangement of protein subunits into sheets, with the alpha-helices normal to the plane of the sheet. This phase transition suggests a model for virion assembly in the bacterial membrane."}
{"id": "PMID:481593", "title": "Local destabilisation of a DNA double helix by a T--T wobble pair.", "content": "Nuclear magnetic resonance is a technique which permits direct observation of the Waton--Click hydrogen-bonded ring imino protons (guanine N1H and thymine N3H). As the formation and disruption of hydrogen bonds of double-helical RNA and DNA structures are key events during various biological processes, NMR thus provides a useful tool for studying the fluctuational mobility of the individual base pairs. Indeed, several NMR studies of oligo- and polynucleotides have been carried out to probe the structure and dynamics of nucleic acids in solution (for a review see ref. 1). The present study constitutes the first part of our attempt to assess the influence of non-complementary base pairs on the stability of nucleic acid double helices. We report the spectral assignment and temperature-dependent NMR profiles of the hydrogen-bonded imino protons of the two DNA fragments shown in Fig. 1. The assignment is based solely on experimental grounds using the principle of chemical modification. It will be demonstrated that the introduction of a non-complementary (wobble) base pair in a DNA duplex introduces an extra melting site in addition to the sequential melting which starts with the terminal base pairs in the double helix structure.", "contents": "Local destabilisation of a DNA double helix by a T--T wobble pair. Nuclear magnetic resonance is a technique which permits direct observation of the Waton--Click hydrogen-bonded ring imino protons (guanine N1H and thymine N3H). As the formation and disruption of hydrogen bonds of double-helical RNA and DNA structures are key events during various biological processes, NMR thus provides a useful tool for studying the fluctuational mobility of the individual base pairs. Indeed, several NMR studies of oligo- and polynucleotides have been carried out to probe the structure and dynamics of nucleic acids in solution (for a review see ref. 1). The present study constitutes the first part of our attempt to assess the influence of non-complementary base pairs on the stability of nucleic acid double helices. We report the spectral assignment and temperature-dependent NMR profiles of the hydrogen-bonded imino protons of the two DNA fragments shown in Fig. 1. The assignment is based solely on experimental grounds using the principle of chemical modification. It will be demonstrated that the introduction of a non-complementary (wobble) base pair in a DNA duplex introduces an extra melting site in addition to the sequential melting which starts with the terminal base pairs in the double helix structure."}
{"id": "PMID:481594", "title": "Thromboxane molecules do not adopt the prostaglandin hairpin conformation.", "content": "The hairpin conformational hypothesis has been proposed to rationalise much of the structure-activity and receptor-binding data which have accumulated for the prostaglandin (PG) hormones. The hairpin conformation, thought to be necessary for PG activity, requires that the alpha- and omega-chains of the molecule be extended and in parallel alignment, separated by a van der Waals contact distance for the full length of the chains, with the ends of the chains approximately 5.5 A apart. The similarity between the structures of the thromboxanes (TXs) and the PGs suggests that the profile of activity of TXs, like that of PGs, centres on subtle conformational variation of the hairpin geometry. Thromboxane B2 (TXB2) is a stable hydrolysis product of a highly reactive, short-lived intermediate, thromboxane A2 (TXA2), which is formed from the prostaglandin endoperoxide (PGH2) as indicated in Fig. 1. An examination of molecular models of TXA2 and TXB2 suggests that the structural differences between the ring moieties may have much less influence in altering the side-chain conformation of TXs than do substitutents on the relatively more flexible cyclopentane ring of a PG molecule. We report here the first diffraction analysis of a thromboxane structure and note that the molecular conformation is not hairpin shaped.", "contents": "Thromboxane molecules do not adopt the prostaglandin hairpin conformation. The hairpin conformational hypothesis has been proposed to rationalise much of the structure-activity and receptor-binding data which have accumulated for the prostaglandin (PG) hormones. The hairpin conformation, thought to be necessary for PG activity, requires that the alpha- and omega-chains of the molecule be extended and in parallel alignment, separated by a van der Waals contact distance for the full length of the chains, with the ends of the chains approximately 5.5 A apart. The similarity between the structures of the thromboxanes (TXs) and the PGs suggests that the profile of activity of TXs, like that of PGs, centres on subtle conformational variation of the hairpin geometry. Thromboxane B2 (TXB2) is a stable hydrolysis product of a highly reactive, short-lived intermediate, thromboxane A2 (TXA2), which is formed from the prostaglandin endoperoxide (PGH2) as indicated in Fig. 1. An examination of molecular models of TXA2 and TXB2 suggests that the structural differences between the ring moieties may have much less influence in altering the side-chain conformation of TXs than do substitutents on the relatively more flexible cyclopentane ring of a PG molecule. We report here the first diffraction analysis of a thromboxane structure and note that the molecular conformation is not hairpin shaped."}
{"id": "PMID:481597", "title": "Isolation, cloning and sequence analysis of the cDNA for the alpha-subunit of human chorionic gonadotropin.", "content": "A 621-base pair fragment of the cDNA for the alpha-subunit of human chorionic gonadotropin has been isolated by cloning in a plasmid vector, and the complete nucleotide sequence determined. The entire coding region, including the 24-amino acid pre-sequence and most of the untranslated regions, are present in the fragment.", "contents": "Isolation, cloning and sequence analysis of the cDNA for the alpha-subunit of human chorionic gonadotropin. A 621-base pair fragment of the cDNA for the alpha-subunit of human chorionic gonadotropin has been isolated by cloning in a plasmid vector, and the complete nucleotide sequence determined. The entire coding region, including the 24-amino acid pre-sequence and most of the untranslated regions, are present in the fragment."}
{"id": "PMID:481598", "title": "Behavioural access to short-term memory in bees.", "content": "Memory formation proceeds in temporal phases which differ in their effectiveness in controlling subsequent behavior and in their susceptibility to amnestic treatment. The initial phase of memory formation, frequently termed short-term memory, is generally considered a necessary precursor to long-term memory. However, the course of short-term memory differs widely between animal species and is dependent on experimental procedure. Information may even bypass the short-term phase en route to the long-term one. Experiments reported here using honey bees in a behavioural learning situation suggest that the greatest significance of short-term memory is its function as a mode of memory storage which may be altered effectively by new and contradictory information. Freely flying honey bees were presented two colour alternatives and rewarded on first one and then the other in a reversal learning paradigm. Subsequent colour preference was dependent on the interval between the two trials. Several new features of short-term memory are described. It is concluded that a single mechanisms of short- to long-term memory transfer cannot account for the observed bimodal interval dependent behaviour. Two mechanisms are proposed.", "contents": "Behavioural access to short-term memory in bees. Memory formation proceeds in temporal phases which differ in their effectiveness in controlling subsequent behavior and in their susceptibility to amnestic treatment. The initial phase of memory formation, frequently termed short-term memory, is generally considered a necessary precursor to long-term memory. However, the course of short-term memory differs widely between animal species and is dependent on experimental procedure. Information may even bypass the short-term phase en route to the long-term one. Experiments reported here using honey bees in a behavioural learning situation suggest that the greatest significance of short-term memory is its function as a mode of memory storage which may be altered effectively by new and contradictory information. Freely flying honey bees were presented two colour alternatives and rewarded on first one and then the other in a reversal learning paradigm. Subsequent colour preference was dependent on the interval between the two trials. Several new features of short-term memory are described. It is concluded that a single mechanisms of short- to long-term memory transfer cannot account for the observed bimodal interval dependent behaviour. Two mechanisms are proposed."}
{"id": "PMID:481600", "title": "A new neuronal marker identified by phosphorylcholine-binding myeloma proteins.", "content": "The difficulty of working with the intact brain in vivo has led to the increasing use of nerve cell cultures in neurobiology. However, dissociated cells cannot be unambiguously identified by morphological criteria before the third week in culture, for it is not until then that the basic morphology and size of neurones become stable so that these and other cell types can be easily distinguished. However, cultured neurones can be identified by various cytochemical techniques based on (1) the detection of neurotransmitters or receptors for transmitters, (2) the presence of the Thy 1 antigen and the receptor for tetanus toxin, which are present on the membrane of most neurones, and (3) the presence in neurones of neurone-specific enolase (NSE), a cytoplasmic enzyme, which can only be identified on fixed specimens. Furthermore, other cell types in culture can also be specifically labelled. For instance, antisera to galactocerebroside bind selectively to oligodendrocytes, and antibodies to a neural tumour bind selectively to Schwann cells. We report here the selective interaction of phosphorylcholine-binding myeloma proteins (PC-BMP) with mouse neurones in culture and  in suspension. Phosphorylcholine (PC) is found as part of lecithin and sphingomyelin molecules in variable amounts in eukaryotic and prokaryotic membranes, including plasma membranes.", "contents": "A new neuronal marker identified by phosphorylcholine-binding myeloma proteins. The difficulty of working with the intact brain in vivo has led to the increasing use of nerve cell cultures in neurobiology. However, dissociated cells cannot be unambiguously identified by morphological criteria before the third week in culture, for it is not until then that the basic morphology and size of neurones become stable so that these and other cell types can be easily distinguished. However, cultured neurones can be identified by various cytochemical techniques based on (1) the detection of neurotransmitters or receptors for transmitters, (2) the presence of the Thy 1 antigen and the receptor for tetanus toxin, which are present on the membrane of most neurones, and (3) the presence in neurones of neurone-specific enolase (NSE), a cytoplasmic enzyme, which can only be identified on fixed specimens. Furthermore, other cell types in culture can also be specifically labelled. For instance, antisera to galactocerebroside bind selectively to oligodendrocytes, and antibodies to a neural tumour bind selectively to Schwann cells. We report here the selective interaction of phosphorylcholine-binding myeloma proteins (PC-BMP) with mouse neurones in culture and  in suspension. Phosphorylcholine (PC) is found as part of lecithin and sphingomyelin molecules in variable amounts in eukaryotic and prokaryotic membranes, including plasma membranes."}
{"id": "PMID:481602", "title": "Erythropoietin-stimulated erythropoiesis in long-term bone marrow culture.", "content": "The proliferation of multipotential haematopoietic stem cells (CFU-S) is possible in some long-term bone marrow cultures. Granulocyte and macrophage progenitor (CFU-C) and megakaryocyte precursor cells (CFU-M) are present in these cultures and undergo full development into mature cells. In contrast, while immature erythroid progenitors ('early' BFU-E) are maintained in long-term culture, none of the more differentiated progeny (CFU-E) have been detected, and no morphologically recognisable erythroid cells have been observed. We now describe a modified culture system in which the 'early' BFU-E develop into 'late' BFU-E in response to added erythropoietin. Further maturation of these cells into CFU-E and non-nucleated erythrocytes can be achieved by mechanical agitation of the long-term cultures or by transferring the cells into dishes which do not allow cell attachment to occur.", "contents": "Erythropoietin-stimulated erythropoiesis in long-term bone marrow culture. The proliferation of multipotential haematopoietic stem cells (CFU-S) is possible in some long-term bone marrow cultures. Granulocyte and macrophage progenitor (CFU-C) and megakaryocyte precursor cells (CFU-M) are present in these cultures and undergo full development into mature cells. In contrast, while immature erythroid progenitors ('early' BFU-E) are maintained in long-term culture, none of the more differentiated progeny (CFU-E) have been detected, and no morphologically recognisable erythroid cells have been observed. We now describe a modified culture system in which the 'early' BFU-E develop into 'late' BFU-E in response to added erythropoietin. Further maturation of these cells into CFU-E and non-nucleated erythrocytes can be achieved by mechanical agitation of the long-term cultures or by transferring the cells into dishes which do not allow cell attachment to occur."}
{"id": "PMID:481603", "title": "Vascular endothelial and smooth muscle cells in culture selectively release adenine nucleotides.", "content": "Endothelial cells in culture can modulate platelet aggregation and vascular tone, in part by producing prostacyclin (PGI2), a powerful vasodilator and inhibitor of platelet aggregation, but also by their ecto-ADPase activity, which initiates the conversion of pro-aggregating ADP to adenosine, a potent vasodilator and platelet inhibitor. We have now demonstrated that cultured aortic endothelial cells exposed to trypsin, thrombin or other stimuli can liberate a high proportion of their adenine nucleotides without substantial loss of lactate dehydrogenase. ADP rapidly accumulates extracellularly, reaching biologically active concentrations before there is further breakdown to adenosine. Whether this selective release of nucleotides is a response to damage, or whether it represents a specific secretory mechanism remains to be resolved. Cultured aortic smooth muscle cells can secrete adenine nucleotides in a similar manner, but extracellular conversion to adenosine occurs much faster.", "contents": "Vascular endothelial and smooth muscle cells in culture selectively release adenine nucleotides. Endothelial cells in culture can modulate platelet aggregation and vascular tone, in part by producing prostacyclin (PGI2), a powerful vasodilator and inhibitor of platelet aggregation, but also by their ecto-ADPase activity, which initiates the conversion of pro-aggregating ADP to adenosine, a potent vasodilator and platelet inhibitor. We have now demonstrated that cultured aortic endothelial cells exposed to trypsin, thrombin or other stimuli can liberate a high proportion of their adenine nucleotides without substantial loss of lactate dehydrogenase. ADP rapidly accumulates extracellularly, reaching biologically active concentrations before there is further breakdown to adenosine. Whether this selective release of nucleotides is a response to damage, or whether it represents a specific secretory mechanism remains to be resolved. Cultured aortic smooth muscle cells can secrete adenine nucleotides in a similar manner, but extracellular conversion to adenosine occurs much faster."}
{"id": "PMID:481606", "title": "Pretreatment with acetylaminofluorene enhances the repair of O6-methylguanine in DNA.", "content": "Epidemiological and experimental evidence suggests that the interplay of environmental factors may be particularly relevant to the induction of cancer in man. We have investigated the possible interrelationships, at the level of DNA repair, of two chemically different hepatocarcinogens administered to rats. Animals were pretreated for several weeks by inclusion of 2-acetylaminofluorene (AAF) in the diet and a study was made of the capacity of liver to repair lesions subsequently introduced into DNA by a pulse of dimethylnitrosamine (DMN). Of particular interest was the repair of adducts at the O6 position of guanine as this product has been implicated as a critical reaction site for carcinogenesis by the monofunctional alkylating agents. We show here that the capacity of liver to repair O6-methylguanine in DNA is enhanced by prolonged exposure of rats to the chemically unrelated agent, AAF.", "contents": "Pretreatment with acetylaminofluorene enhances the repair of O6-methylguanine in DNA. Epidemiological and experimental evidence suggests that the interplay of environmental factors may be particularly relevant to the induction of cancer in man. We have investigated the possible interrelationships, at the level of DNA repair, of two chemically different hepatocarcinogens administered to rats. Animals were pretreated for several weeks by inclusion of 2-acetylaminofluorene (AAF) in the diet and a study was made of the capacity of liver to repair lesions subsequently introduced into DNA by a pulse of dimethylnitrosamine (DMN). Of particular interest was the repair of adducts at the O6 position of guanine as this product has been implicated as a critical reaction site for carcinogenesis by the monofunctional alkylating agents. We show here that the capacity of liver to repair O6-methylguanine in DNA is enhanced by prolonged exposure of rats to the chemically unrelated agent, AAF."}
{"id": "PMID:481609", "title": "Evidence for a central postsynaptic action of clonidine.", "content": "Intravenous administration of clonidine (1--100 micrograms/kg) produces a dose-dependent mydriasis in cats by inhibition of parasympathetic tone to the iris. The magnitude of CNS-induced pupillary dilation was similar in both normal anaesthetized cats and in anaesthetized preparations pretreated with reserpine (5 mg/kg i.p.) and alpha-methyl-p-tyrosine (2 x 300 mg/kg i.p.). Pretreatment reduced the concentrations of noradrenaline, dopamine and serotonin to less than 3% of that control levels in most parts of the CNS in which these amines were measured. Clonidine produced bradycardia in control animals but not in pretreated cats. In amine depleted animals in which only one eye was innervated by the ciliary nerves (parasympathetic), clonidine produced mydriasis only on the innervated side. These experiments confirm our previous observations that clonidine produces mydriasis in the cat by means of inhibition of parasympathetic tone to the iris. It is concluded that if clonidine produces this effect by stimulating noradrenergic, dopaminergic or serotonergic receptors, then clonidine exerts its centrally-induced mydriatic effect by acting on post-synaptic mechanisms.", "contents": "Evidence for a central postsynaptic action of clonidine. Intravenous administration of clonidine (1--100 micrograms/kg) produces a dose-dependent mydriasis in cats by inhibition of parasympathetic tone to the iris. The magnitude of CNS-induced pupillary dilation was similar in both normal anaesthetized cats and in anaesthetized preparations pretreated with reserpine (5 mg/kg i.p.) and alpha-methyl-p-tyrosine (2 x 300 mg/kg i.p.). Pretreatment reduced the concentrations of noradrenaline, dopamine and serotonin to less than 3% of that control levels in most parts of the CNS in which these amines were measured. Clonidine produced bradycardia in control animals but not in pretreated cats. In amine depleted animals in which only one eye was innervated by the ciliary nerves (parasympathetic), clonidine produced mydriasis only on the innervated side. These experiments confirm our previous observations that clonidine produces mydriasis in the cat by means of inhibition of parasympathetic tone to the iris. It is concluded that if clonidine produces this effect by stimulating noradrenergic, dopaminergic or serotonergic receptors, then clonidine exerts its centrally-induced mydriatic effect by acting on post-synaptic mechanisms."}
{"id": "PMID:481611", "title": "The influence of (+)-propranolol on the inotropic effect of dihydro-ouabain in relation to stimulation frequency.", "content": "1. The influence of (+)-propranolol, 10(-6) to 4 x 10(-5) mol/l, upon the positive inotropic effect of dihydro-ouabain was studied on guinea-pig papillary muscle. At 1 Hz, negative inotropically effective concentrations of (+)-propranolol decreased the inotropic effectiveness of the glycoside. 2. For equivalent diminution of the basal force of contraction, either by reduction of stimulation frequency or by increasing concentrations of (+)-propranolol, a similar decrease in the inotropic effectiveness of dihydro-ouabain was observed. In contract, reduction of [Ca2+]o  had almost no effect on the effectiveness of the glycoside. 3. The inhibitory effect of (+)-propranolol on the inotropic effectiveness of dihydro-ouabain declined with reduction of stimulation frequency. At any stimulation frequency tested, the inhibitory effect of (+)-propranolol was proportional to the effect of a reduction of that particular frequency to the rested-state condition in the absence of (+)-propranolol. 4. The results are consistent with the hypothesis that the inhibition of the excitation-coupled sodium influx by (+)-propranolol is the cause of its inhibiting effect on the effectiveness of dihydro-ouabain.", "contents": "The influence of (+)-propranolol on the inotropic effect of dihydro-ouabain in relation to stimulation frequency. 1. The influence of (+)-propranolol, 10(-6) to 4 x 10(-5) mol/l, upon the positive inotropic effect of dihydro-ouabain was studied on guinea-pig papillary muscle. At 1 Hz, negative inotropically effective concentrations of (+)-propranolol decreased the inotropic effectiveness of the glycoside. 2. For equivalent diminution of the basal force of contraction, either by reduction of stimulation frequency or by increasing concentrations of (+)-propranolol, a similar decrease in the inotropic effectiveness of dihydro-ouabain was observed. In contract, reduction of [Ca2+]o  had almost no effect on the effectiveness of the glycoside. 3. The inhibitory effect of (+)-propranolol on the inotropic effectiveness of dihydro-ouabain declined with reduction of stimulation frequency. At any stimulation frequency tested, the inhibitory effect of (+)-propranolol was proportional to the effect of a reduction of that particular frequency to the rested-state condition in the absence of (+)-propranolol. 4. The results are consistent with the hypothesis that the inhibition of the excitation-coupled sodium influx by (+)-propranolol is the cause of its inhibiting effect on the effectiveness of dihydro-ouabain."}
{"id": "PMID:481612", "title": "Perfusion of the fourth cerebral ventricle with fentanyl induces naloxone-reversible bradycardia, hypotension, and EEG synchronisation in conscious dogs.", "content": "Injection of fentanyl into the cisterna magna of the brain leads to hypotension and bradycardia in anesthetised dogs. To determine if this effect is related to the recently discovered opiate receptors fentanyl was perfused in increasing concentrations (2.5-20 microgram/ml) through the cerebro-ventricular system in conscious dogs. Blood pressure was recorded continuously from a punctured exteriorised carotid artery; heart rate was derived from the ECG. Baroreflex activity was tested repeatedly by clamping of both common carotid arteries. Cerebral activity was evaluated from the EEG. Perfusion of the fourth cerebral ventricle resulted in a concentration-related fall in heart rate by 43% and in an inhibition of the reflex response of heart rate to carotid clamping. In contrast, blood pressure fell only moderately by 14% and its reflex response was well maintained. The EEG pattern changed from frequencies in the beta-band (awake control) to gradual synchronisation with delta-activity corresponding to behavioural signs of tranquilisation and sleep-like states. All these effects were reversed by naloxone. No effects were seen when fentanyl was perfused through the lateral ventricles and third ventricle although this yielded similar serum concentration as after perfusion of the fourth ventricle. It is concluded that opiate receptors bordering the fourth cerebral ventricle mediate the cardiovascular and hypnotic action of fentanyl.", "contents": "Perfusion of the fourth cerebral ventricle with fentanyl induces naloxone-reversible bradycardia, hypotension, and EEG synchronisation in conscious dogs. Injection of fentanyl into the cisterna magna of the brain leads to hypotension and bradycardia in anesthetised dogs. To determine if this effect is related to the recently discovered opiate receptors fentanyl was perfused in increasing concentrations (2.5-20 microgram/ml) through the cerebro-ventricular system in conscious dogs. Blood pressure was recorded continuously from a punctured exteriorised carotid artery; heart rate was derived from the ECG. Baroreflex activity was tested repeatedly by clamping of both common carotid arteries. Cerebral activity was evaluated from the EEG. Perfusion of the fourth cerebral ventricle resulted in a concentration-related fall in heart rate by 43% and in an inhibition of the reflex response of heart rate to carotid clamping. In contrast, blood pressure fell only moderately by 14% and its reflex response was well maintained. The EEG pattern changed from frequencies in the beta-band (awake control) to gradual synchronisation with delta-activity corresponding to behavioural signs of tranquilisation and sleep-like states. All these effects were reversed by naloxone. No effects were seen when fentanyl was perfused through the lateral ventricles and third ventricle although this yielded similar serum concentration as after perfusion of the fourth ventricle. It is concluded that opiate receptors bordering the fourth cerebral ventricle mediate the cardiovascular and hypnotic action of fentanyl."}
{"id": "PMID:481613", "title": "Failure of chronic lithium treatment to block tricyclic antidepressant-induced 5-HT supersensitivity.", "content": "The ability of chronic lithium administration to modify tricylic antidepressant-induced supersensitivity development in cells receiving 5-hydroxytryptamine (5-HT) input was investigated using microiontophoretic techniques. In these experiments, chronic chlorimipramine (or imipramine) administration for a period of 14 days resulted in a 5-fold increase in the sensitivity of hippocampal pyramidal cells to iontophoretically applied 5-HT. This supersensitivity was not blocked by the concurrent administration of lithium. The data suggests that blockade of supersensitivity development by lithium as previously demonstrated in the dopamine system may not be generalized to all central amine systems.", "contents": "Failure of chronic lithium treatment to block tricyclic antidepressant-induced 5-HT supersensitivity. The ability of chronic lithium administration to modify tricylic antidepressant-induced supersensitivity development in cells receiving 5-hydroxytryptamine (5-HT) input was investigated using microiontophoretic techniques. In these experiments, chronic chlorimipramine (or imipramine) administration for a period of 14 days resulted in a 5-fold increase in the sensitivity of hippocampal pyramidal cells to iontophoretically applied 5-HT. This supersensitivity was not blocked by the concurrent administration of lithium. The data suggests that blockade of supersensitivity development by lithium as previously demonstrated in the dopamine system may not be generalized to all central amine systems."}
{"id": "PMID:481614", "title": "Studies on tryptophan accumulation in brain during methiothepin-induced enhancement of 5-hydroxyindole synthesis.", "content": "The elevation of brain tryptophan, 5-hydroxytryptophan and 5-hydroxyindoles (serotonin + 5-hydroxyindole acetic acid) that results from a tryptophan load is potentiated by prior administration of methiothepin, a serotonin receptor antagonist. Co-administration of valine with tryptophan attenuates these effects even in animals receiving methiothepin pretreatment. Administration of methiothepin and tryptophan to rats with widespread reduction of brain 5-hydroxyindole levels resulting from raphe lesions or 5,7-dihydroxytryptamine pretreatment still enabled brain tryptophan levels to rise considerably above the sum of increases found in animals receiving one or the other. Following transection of the spinal cord, the cranial portion still exhibited enhanced uptake of tryptophan and 5-hydroxyindole synthesis following methiothepin plus tryptophan treatment, however, both these events were absent in the caudal segment. Apparently, enhanced tryptophan uptake can proceed in the presence of minimal neuronal activity; however, when nerve impulse flow is eliminated, both 5-hydroxyindole synthesis and tryptophan uptake is impaired.", "contents": "Studies on tryptophan accumulation in brain during methiothepin-induced enhancement of 5-hydroxyindole synthesis. The elevation of brain tryptophan, 5-hydroxytryptophan and 5-hydroxyindoles (serotonin + 5-hydroxyindole acetic acid) that results from a tryptophan load is potentiated by prior administration of methiothepin, a serotonin receptor antagonist. Co-administration of valine with tryptophan attenuates these effects even in animals receiving methiothepin pretreatment. Administration of methiothepin and tryptophan to rats with widespread reduction of brain 5-hydroxyindole levels resulting from raphe lesions or 5,7-dihydroxytryptamine pretreatment still enabled brain tryptophan levels to rise considerably above the sum of increases found in animals receiving one or the other. Following transection of the spinal cord, the cranial portion still exhibited enhanced uptake of tryptophan and 5-hydroxyindole synthesis following methiothepin plus tryptophan treatment, however, both these events were absent in the caudal segment. Apparently, enhanced tryptophan uptake can proceed in the presence of minimal neuronal activity; however, when nerve impulse flow is eliminated, both 5-hydroxyindole synthesis and tryptophan uptake is impaired."}
{"id": "PMID:481615", "title": "Effect of age on levels of diazepam in plasma and brain of rats.", "content": "The pharmacokinetics and distribution in brain and cerebellum of diazepam after a single dose were studied in middle aged (6 months) and old (18 months) rats. Following the single intravenous bolus of 5 mg/kg diazepam was eliminated more slowly in old rats (T 1/2 (beta) = 3.1 H) than in middle aged rats (1.4H). This was due to an increase in the apparent volume of distribution Vd beta from 11.0 l/kg (control rats) to 29.5 l/kg. Concentrations of diazepam in brain and cerebellum were in the same range (0.5-1.1 ng/mg) in both groups after this dose. We conclude that the distribution of diazepam is age-dependent which might be due to an altered body composition.", "contents": "Effect of age on levels of diazepam in plasma and brain of rats. The pharmacokinetics and distribution in brain and cerebellum of diazepam after a single dose were studied in middle aged (6 months) and old (18 months) rats. Following the single intravenous bolus of 5 mg/kg diazepam was eliminated more slowly in old rats (T 1/2 (beta) = 3.1 H) than in middle aged rats (1.4H). This was due to an increase in the apparent volume of distribution Vd beta from 11.0 l/kg (control rats) to 29.5 l/kg. Concentrations of diazepam in brain and cerebellum were in the same range (0.5-1.1 ng/mg) in both groups after this dose. We conclude that the distribution of diazepam is age-dependent which might be due to an altered body composition."}
{"id": "PMID:481655", "title": "Variations in serum complement following inulin infusion in man.", "content": "Complement levels were determined in 12 normal human volunteers while receiving intravenous infusions of inulin for determination of standard inulin clearances. Significant decreases in beta IC globulin and total hemolytic complement activity were observed when inulin was infused, but not following infusion of saline as a control. These effects were noted only when steady-state inulin levels were greater than 26 mg%, suggesting a dose-dependent response. No evidence of increased coagulation was noted as measured by fibrinogen and fibrin degradation products. Since C4 levels remained largely unchanged following inulin infusion, it was concluded that the results most likely occurred through activation of the alternate complement pathway.", "contents": "Variations in serum complement following inulin infusion in man. Complement levels were determined in 12 normal human volunteers while receiving intravenous infusions of inulin for determination of standard inulin clearances. Significant decreases in beta IC globulin and total hemolytic complement activity were observed when inulin was infused, but not following infusion of saline as a control. These effects were noted only when steady-state inulin levels were greater than 26 mg%, suggesting a dose-dependent response. No evidence of increased coagulation was noted as measured by fibrinogen and fibrin degradation products. Since C4 levels remained largely unchanged following inulin infusion, it was concluded that the results most likely occurred through activation of the alternate complement pathway."}
{"id": "PMID:481656", "title": "Changes in total body calcium on prolonged maintenance hemodialysis with high and low dialysate calcium.", "content": "In vivo neutron activation analysis was used to measure sequential changes in total body calcium in 25 unselected patients with end-stage renal disease on dialysis for a period as long as 5 years. In 7 unselected patients (group 1), dialysis with a calcium solution of 5.0 mg/100 ml resulted in significant calculated daily loss of calcium (-321 +/- SEM 76 mg/day) while dialysis with 6.5 mg/100 ml resulted in a significant calculated calcium gain (182 +/- SEM 103 mg/day; p less than 0.0025). The remaining 18 patients (group 2) were dialyzed with a dialysate calcium of 6.5 mg/100 ml and sequential total body calcium measurements showed no significant calcium losses (-12 +/- SEM 68 mg/day) for a period as long as 2 years.", "contents": "Changes in total body calcium on prolonged maintenance hemodialysis with high and low dialysate calcium. In vivo neutron activation analysis was used to measure sequential changes in total body calcium in 25 unselected patients with end-stage renal disease on dialysis for a period as long as 5 years. In 7 unselected patients (group 1), dialysis with a calcium solution of 5.0 mg/100 ml resulted in significant calculated daily loss of calcium (-321 +/- SEM 76 mg/day) while dialysis with 6.5 mg/100 ml resulted in a significant calculated calcium gain (182 +/- SEM 103 mg/day; p less than 0.0025). The remaining 18 patients (group 2) were dialyzed with a dialysate calcium of 6.5 mg/100 ml and sequential total body calcium measurements showed no significant calcium losses (-12 +/- SEM 68 mg/day) for a period as long as 2 years."}
{"id": "PMID:481658", "title": "Platelets and platelet function in patients with chronic uremia on maintenance hemodialysis.", "content": "In 20 chronic uremic patients on maintenance hemodialysis, who were not taking any medication known to affect platelet function, the following investigations were carried out: platelet count, fibrin/fibrinogen degradation products, fibrinogen and plasminogen concentration, platelet adhesiveness, clot retraction and platelet aggregation induced by ADP, ristocetin, fibrinogen, collagen and epinephrine. The only significant abnormal result was a decreased clot retraction. We consider many cases of so-called uremic bleeding to be caused by the medication taken and conclude that on well-controlled hemodialysis treatment, bleeding tendency should not be a major problem.", "contents": "Platelets and platelet function in patients with chronic uremia on maintenance hemodialysis. In 20 chronic uremic patients on maintenance hemodialysis, who were not taking any medication known to affect platelet function, the following investigations were carried out: platelet count, fibrin/fibrinogen degradation products, fibrinogen and plasminogen concentration, platelet adhesiveness, clot retraction and platelet aggregation induced by ADP, ristocetin, fibrinogen, collagen and epinephrine. The only significant abnormal result was a decreased clot retraction. We consider many cases of so-called uremic bleeding to be caused by the medication taken and conclude that on well-controlled hemodialysis treatment, bleeding tendency should not be a major problem."}
{"id": "PMID:481659", "title": "Perinephric abscess in patients with polycystic kidney disease undergoing chronic hemodialysis.", "content": "5 patients with polycystic kidney disease undergoing chronic hemodialysis who developed perinephric abscesses are described. Gallium-67 scintigraphy was helpful in making a diagnosis in 2 of these patients. All 5 patients initially presented with urinary tract infections. Perinephric abscess became evident over a variable period of time (2--28 days) following completion of antibiotic therapy for their urinary tract infection. Gallium-67 scintigraphy appears useful in detecting this complication, and nephrectomy should be considered once the diagnosis is confirmed.", "contents": "Perinephric abscess in patients with polycystic kidney disease undergoing chronic hemodialysis. 5 patients with polycystic kidney disease undergoing chronic hemodialysis who developed perinephric abscesses are described. Gallium-67 scintigraphy was helpful in making a diagnosis in 2 of these patients. All 5 patients initially presented with urinary tract infections. Perinephric abscess became evident over a variable period of time (2--28 days) following completion of antibiotic therapy for their urinary tract infection. Gallium-67 scintigraphy appears useful in detecting this complication, and nephrectomy should be considered once the diagnosis is confirmed."}
{"id": "PMID:481661", "title": "Interrelationship between acetylcholine and prostaglandins in the control of sodium excretion and renin secretion in anesthetized dogs. I.", "content": "The interrelationships between acetylcholine (ACH) and prostaglandins in the control of sodium excretion (UNaV) and of renin secretion (RS) were examined in unilaterally-nephrectomized, anesthetized dogs with or without treatment with indomethacin, an inhibitor of prostaglandin synthetase. Inrarenal infusion of ACH (40 microgram/min) in control animals produced diuresis and natriuresis. UNaV was 9.2 +/- 3.1 muEq/min during control periods and was 74.7 +/- 31.6 muEq/min after 40 min of infusion of ACH (p less than 0.05). UNaV was still 48.0 +/- 16.8 muEq/min after 100 min of infusion of ACH (p less than 0.05). RS rate was 930.6 +/- 188.5 U/min during control periods, and was 737.4 +/- 220.3 U/min after 100 min of infusion of ACH (p greater than 0.1). In the dogs treated with indomethacin, the natriuresis produced by ACH could not be sustained. UNaV was 46.6 +/- 12.1 muEq/min before and was 34.7 +/- 12.8 muEq/min 100 min following the infusion of ACH (p greater than 0.1). Arterial plasma renin activity was 3.29 +/- 0.83 ng/ml/h before and was 20.97 +/- 7.78 ng/ml/h after 100 min of infusion of ACH (p less than 0.025). The data suggest that prostaglandins are involved in the sodium excretion and the renin secretion produced by acetylcholine.", "contents": "Interrelationship between acetylcholine and prostaglandins in the control of sodium excretion and renin secretion in anesthetized dogs. I. The interrelationships between acetylcholine (ACH) and prostaglandins in the control of sodium excretion (UNaV) and of renin secretion (RS) were examined in unilaterally-nephrectomized, anesthetized dogs with or without treatment with indomethacin, an inhibitor of prostaglandin synthetase. Inrarenal infusion of ACH (40 microgram/min) in control animals produced diuresis and natriuresis. UNaV was 9.2 +/- 3.1 muEq/min during control periods and was 74.7 +/- 31.6 muEq/min after 40 min of infusion of ACH (p less than 0.05). UNaV was still 48.0 +/- 16.8 muEq/min after 100 min of infusion of ACH (p less than 0.05). RS rate was 930.6 +/- 188.5 U/min during control periods, and was 737.4 +/- 220.3 U/min after 100 min of infusion of ACH (p greater than 0.1). In the dogs treated with indomethacin, the natriuresis produced by ACH could not be sustained. UNaV was 46.6 +/- 12.1 muEq/min before and was 34.7 +/- 12.8 muEq/min 100 min following the infusion of ACH (p greater than 0.1). Arterial plasma renin activity was 3.29 +/- 0.83 ng/ml/h before and was 20.97 +/- 7.78 ng/ml/h after 100 min of infusion of ACH (p less than 0.025). The data suggest that prostaglandins are involved in the sodium excretion and the renin secretion produced by acetylcholine."}
{"id": "PMID:481662", "title": "Renal effects of mannitol in the early stage of glycerol-induced acute renal failure in the rat.", "content": "The effects of mannitol on the hemodynamics and excretory function of the kidney in control rats and in rats with acute renal failure (ARF), induced by glycerol, were studied using clearance, micropuncture and flowmeter techniques. In control animals, 20% mannitol significantly increased renal blood flow (RBF), urinary volume and sodium excretion and decreased urinary sodium concentration and fractional tubular sodium reabsorption. Glomerular filtration rate (GFR) and effective filtration pressure remained unchanged. 50% glycerol (10 ml/kg bw) was injected intramuscularly in other groups of rats to induce ARF. Functional impairment of the glycerol-treated animals consisted in a significant decrease in urinary sodium concentration, RBF, GFR and effective filtration pressure of superficial nephrons. Urea concentration in arterial plasma and urine volume increased significantly. Infusion of 20% mannitol into these animals led to a significant further decrease in GFR and net filtration pressure, but not in RBF. Urine volume increased to the same degree as in controls.", "contents": "Renal effects of mannitol in the early stage of glycerol-induced acute renal failure in the rat. The effects of mannitol on the hemodynamics and excretory function of the kidney in control rats and in rats with acute renal failure (ARF), induced by glycerol, were studied using clearance, micropuncture and flowmeter techniques. In control animals, 20% mannitol significantly increased renal blood flow (RBF), urinary volume and sodium excretion and decreased urinary sodium concentration and fractional tubular sodium reabsorption. Glomerular filtration rate (GFR) and effective filtration pressure remained unchanged. 50% glycerol (10 ml/kg bw) was injected intramuscularly in other groups of rats to induce ARF. Functional impairment of the glycerol-treated animals consisted in a significant decrease in urinary sodium concentration, RBF, GFR and effective filtration pressure of superficial nephrons. Urea concentration in arterial plasma and urine volume increased significantly. Infusion of 20% mannitol into these animals led to a significant further decrease in GFR and net filtration pressure, but not in RBF. Urine volume increased to the same degree as in controls."}
{"id": "PMID:481664", "title": "Some aspects of cellular immunity in untreated and maintenance hemodialysis patients.", "content": "The cellular pattern of skin exudate in untreated uremic patients differed from that in healthy persons. The skin exudate composition in patients on maintenance hemodialysis approached normal values. Such normalization was, however, static: after sensitization with dinitrochlorobenzene--contrary to the control--both treated and untreated patients exhibited no changes in the cellular pattern of exudate. The activity of acid phosphatase in the cells of exudate was highly positive in control, almost normal in dialyzed, but weak in untreated uremic patients. Our investigations confirmed that cellular immunity in untreated and in maintenance hemodialysis patients is suppressed.", "contents": "Some aspects of cellular immunity in untreated and maintenance hemodialysis patients. The cellular pattern of skin exudate in untreated uremic patients differed from that in healthy persons. The skin exudate composition in patients on maintenance hemodialysis approached normal values. Such normalization was, however, static: after sensitization with dinitrochlorobenzene--contrary to the control--both treated and untreated patients exhibited no changes in the cellular pattern of exudate. The activity of acid phosphatase in the cells of exudate was highly positive in control, almost normal in dialyzed, but weak in untreated uremic patients. Our investigations confirmed that cellular immunity in untreated and in maintenance hemodialysis patients is suppressed."}
{"id": "PMID:481665", "title": "The renal handling of amylase in normal man.", "content": "The renal glomerular and tubular transport rate of amylase was studied by measuring the urinary excretion of this protein before and during inhibition of tubular protein reabsorption by lysine. The excretion of amylase was compared with the excretion of albumin, beta-2 microglobulin and free light chains of immunoglobulins. This investigation showed that amylase is reabsorbed by the tubular cells, but only to a very modest degree compared with the reabsorption of the other three proteins. In the case of amylase only about 45% of the filtered molecules are reabsorbed, whereas more than 90% of the filtered amount of the other molecules is reabsorbed by the tubular cells. The excretion of amylase rose after lysine injection only by a factor 1.8, whereas excretion rose by a factor 28 for albumin, 1,500 for beta-2 microglobulin, 16 for kappa chains and 8 for lambda chains. Minimal values for tubular reabsorption were found to be 5.5 +/- (SD) 4.3 U/min for amylase, 174.0 +/- 35.7 micrograms/min for albumin, 90.5 +/- 14.4 micrograms/min for beta-2-microglobulin, 70.4 +/- 17.4 micrograms/min for kappa chains and 24.2 +/- 9.2 micrograms/min for lambda chains.", "contents": "The renal handling of amylase in normal man. The renal glomerular and tubular transport rate of amylase was studied by measuring the urinary excretion of this protein before and during inhibition of tubular protein reabsorption by lysine. The excretion of amylase was compared with the excretion of albumin, beta-2 microglobulin and free light chains of immunoglobulins. This investigation showed that amylase is reabsorbed by the tubular cells, but only to a very modest degree compared with the reabsorption of the other three proteins. In the case of amylase only about 45% of the filtered molecules are reabsorbed, whereas more than 90% of the filtered amount of the other molecules is reabsorbed by the tubular cells. The excretion of amylase rose after lysine injection only by a factor 1.8, whereas excretion rose by a factor 28 for albumin, 1,500 for beta-2 microglobulin, 16 for kappa chains and 8 for lambda chains. Minimal values for tubular reabsorption were found to be 5.5 +/- (SD) 4.3 U/min for amylase, 174.0 +/- 35.7 micrograms/min for albumin, 90.5 +/- 14.4 micrograms/min for beta-2-microglobulin, 70.4 +/- 17.4 micrograms/min for kappa chains and 24.2 +/- 9.2 micrograms/min for lambda chains."}
{"id": "PMID:481678", "title": "Regulatory interrelations between GABA and polyamines. I. Brain GABA levels and polyamine metabolism.", "content": "Elevation of brain GABA levels by GABA-T inhibition is accompanied by a decrease of S-adenosylmethionine decarboxylase activity. This is followed by an increase of ornithine decarboxylase activity and a severalfold increase of brain putrescine levels. Spermidine and spermine levels are not significantly affected under these conditions. These unexpected findings support a regulatory interaction between GABA and polyamine metabolism.", "contents": "Regulatory interrelations between GABA and polyamines. I. Brain GABA levels and polyamine metabolism. Elevation of brain GABA levels by GABA-T inhibition is accompanied by a decrease of S-adenosylmethionine decarboxylase activity. This is followed by an increase of ornithine decarboxylase activity and a severalfold increase of brain putrescine levels. Spermidine and spermine levels are not significantly affected under these conditions. These unexpected findings support a regulatory interaction between GABA and polyamine metabolism."}
{"id": "PMID:481679", "title": "Regulatory interrelations between GABA and polyamines. II. Effect of GABA on ornithine decarboxylase and putrescine levels in cell culture.", "content": "GABA added to rat hepatoma (HTC) cells in spinner culture at the time of induction of cell proliferation increased levels of ornithine decarboxylase (ODC) up to two- to threefold above that of control cells. The increases in ODC were also reflected by concomitant increases of intracellular putrescine levels, while spermidine and spermine were unchanged. GABA seems to have a direct stabilizing effect on ODC, since the turnover of the enzyme was slowed almost twofold when measured in cells treated with 10(-2) M GABA. The stabilizing effect is most pronounced for GABA, although some amino acids such as asparagine, glutamine, and lysine as well as some GABA analogues and homologues also tend to increase ODC but to a significantly lesser extent than GABA itself. GABA metabolites had no effect on ODC. S-Adenosylmethionine decarboxylase and tyrosine aminotransferase were not affected by the presence of GABA. The GABA effect on ODC may be important in certain types of cells for the regulation of polyamine biosynthesis.", "contents": "Regulatory interrelations between GABA and polyamines. II. Effect of GABA on ornithine decarboxylase and putrescine levels in cell culture. GABA added to rat hepatoma (HTC) cells in spinner culture at the time of induction of cell proliferation increased levels of ornithine decarboxylase (ODC) up to two- to threefold above that of control cells. The increases in ODC were also reflected by concomitant increases of intracellular putrescine levels, while spermidine and spermine were unchanged. GABA seems to have a direct stabilizing effect on ODC, since the turnover of the enzyme was slowed almost twofold when measured in cells treated with 10(-2) M GABA. The stabilizing effect is most pronounced for GABA, although some amino acids such as asparagine, glutamine, and lysine as well as some GABA analogues and homologues also tend to increase ODC but to a significantly lesser extent than GABA itself. GABA metabolites had no effect on ODC. S-Adenosylmethionine decarboxylase and tyrosine aminotransferase were not affected by the presence of GABA. The GABA effect on ODC may be important in certain types of cells for the regulation of polyamine biosynthesis."}
{"id": "PMID:481680", "title": "Levels and uptake of taurine in various brain regions after drug-induced generalized convulsions.", "content": "In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The physiological levels of taurine were much higher in mouse brain than in rabbit brain. In vivo the regional levels and the uptake of taurine were not significantly changed after generalized convulsions. Uptake in vivo was lowered in slices obtained from mice treated with metrazol. The lack of effect of metrazol convulsions on cerebral taurine in vivo indicates that further studies are needed to clarify the relationship between taurine, a putative inhibitory transmitter, and epilepsy.", "contents": "Levels and uptake of taurine in various brain regions after drug-induced generalized convulsions. In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The physiological levels of taurine were much higher in mouse brain than in rabbit brain. In vivo the regional levels and the uptake of taurine were not significantly changed after generalized convulsions. Uptake in vivo was lowered in slices obtained from mice treated with metrazol. The lack of effect of metrazol convulsions on cerebral taurine in vivo indicates that further studies are needed to clarify the relationship between taurine, a putative inhibitory transmitter, and epilepsy."}
{"id": "PMID:481681", "title": "Histochemical studies on catecholaminergic cells in the carp retina.", "content": "Histochemical studies on catecholaminergic cells were conducted with the carp (Cyprinus carpio) retina. Catecholamine (CA)-containing cell bodies appear sparsely distributed among amacrine cells in the innermost cellular row of the inner nuclear layer (INL) and occasionally in the outer half part of the inner plexiform layer (IPL); only exceptionally are they found among ganglion cells. The fluorescent cells interspersed with the amacrine cells and in the IPL send their fiber processes toward both the outer plexiform layer (OPL) and the IPL; the fine fibers form dense networks in the INL and IPL. Pretreatment of the fish with intramuscular injection of reserpine (20 hr prior to enucleation) completely depleted CA from the retina. The fluorescence of catecholaminergic cells was enhanced, and the number of fluorescent cells visible was increased, by intravitreous injection of L-DOPA, DA, and NA (3 prior to enucleation). A combination of pretreatment with intramuscular reserpine and intravitreous NA was particularly effective. These results indicate that catecholamines may play an important role in the modulation of the membrane potential of horizontal cells.", "contents": "Histochemical studies on catecholaminergic cells in the carp retina. Histochemical studies on catecholaminergic cells were conducted with the carp (Cyprinus carpio) retina. Catecholamine (CA)-containing cell bodies appear sparsely distributed among amacrine cells in the innermost cellular row of the inner nuclear layer (INL) and occasionally in the outer half part of the inner plexiform layer (IPL); only exceptionally are they found among ganglion cells. The fluorescent cells interspersed with the amacrine cells and in the IPL send their fiber processes toward both the outer plexiform layer (OPL) and the IPL; the fine fibers form dense networks in the INL and IPL. Pretreatment of the fish with intramuscular injection of reserpine (20 hr prior to enucleation) completely depleted CA from the retina. The fluorescence of catecholaminergic cells was enhanced, and the number of fluorescent cells visible was increased, by intravitreous injection of L-DOPA, DA, and NA (3 prior to enucleation). A combination of pretreatment with intramuscular reserpine and intravitreous NA was particularly effective. These results indicate that catecholamines may play an important role in the modulation of the membrane potential of horizontal cells."}
{"id": "PMID:481682", "title": "GABA metabolism in cultured glial cells.", "content": "GABA-transaminase has been characterized in cultured astrocytes. It is identical to the synaptosomal and perikaryal enzyme in terms of charge, molecular weight, and stability, but it differs in its affinity for GABA, which is much higher in the glial compartment. GABA-transaminase has been shown to be inducible by high GABA concentrations, which suggests that astrocytes have the possibility not only to transport GABA but also to metabolize the amino acid which is taken up.", "contents": "GABA metabolism in cultured glial cells. GABA-transaminase has been characterized in cultured astrocytes. It is identical to the synaptosomal and perikaryal enzyme in terms of charge, molecular weight, and stability, but it differs in its affinity for GABA, which is much higher in the glial compartment. GABA-transaminase has been shown to be inducible by high GABA concentrations, which suggests that astrocytes have the possibility not only to transport GABA but also to metabolize the amino acid which is taken up."}
{"id": "PMID:481683", "title": "Dopamine diffusion after microwave fixation at 986 MHz.", "content": "It has been previously demonstrated by us that microwave irradiation at 2450 MHz causes diffusion of dopamine from rat brain regions of high dopamine concentration to contiguous regions low in dopamine. Because we have found that the heating pattern at 986 MHz is opposite to that seen at 2450 MHz, we attempted in the present study to eliminate DA diffusion by heating at 986 MHz. However, dopamine levels were elevated in frontal and parietal cortex, remainder of cortex, and amygdala after sacrifice by microwave irradiation at 986 MHz. Norepinephrine levels were unaffected by sacrifice method. Microwave-induced diffusion of certain substances must be considered in the interpretation of regional neurochemical data.", "contents": "Dopamine diffusion after microwave fixation at 986 MHz. It has been previously demonstrated by us that microwave irradiation at 2450 MHz causes diffusion of dopamine from rat brain regions of high dopamine concentration to contiguous regions low in dopamine. Because we have found that the heating pattern at 986 MHz is opposite to that seen at 2450 MHz, we attempted in the present study to eliminate DA diffusion by heating at 986 MHz. However, dopamine levels were elevated in frontal and parietal cortex, remainder of cortex, and amygdala after sacrifice by microwave irradiation at 986 MHz. Norepinephrine levels were unaffected by sacrifice method. Microwave-induced diffusion of certain substances must be considered in the interpretation of regional neurochemical data."}
{"id": "PMID:481684", "title": "Activity and subcellular distribution of phospholipase A1 from neuronal cell-enriched fractions of the rabbit cerebral cortex.", "content": "Glycerophosphatides, specifically labeled either in the 1 or in the 2 position, were used to measure the activity of neuronal phospholipase A1 and to investigate the subcellular distribution of the enzyme. The microsomes were found to possess the highest phospholipase activity, with a threefold increase as compared to the cell homogenate. A considerable enzymatic activity could still be observed in the plasma membranes isolated from the neuronal-enriched cell fraction. Microsomal phospholipase possessed the highest activity with phosphatidylcholine, whereas phosphatidylserine was cleaved at a much lower rate. The rate of release of labeled fatty acids from the substrates by the microsomal phospholipase decreased with increasing degree of unsaturation of the fatty acids at the 1 position. The presence of plasmalogens and of alkylacyl analogues in the incubation mixture caused an appreciable inhibition of the hydrolysis of the diacyl glycerophosphatides.", "contents": "Activity and subcellular distribution of phospholipase A1 from neuronal cell-enriched fractions of the rabbit cerebral cortex. Glycerophosphatides, specifically labeled either in the 1 or in the 2 position, were used to measure the activity of neuronal phospholipase A1 and to investigate the subcellular distribution of the enzyme. The microsomes were found to possess the highest phospholipase activity, with a threefold increase as compared to the cell homogenate. A considerable enzymatic activity could still be observed in the plasma membranes isolated from the neuronal-enriched cell fraction. Microsomal phospholipase possessed the highest activity with phosphatidylcholine, whereas phosphatidylserine was cleaved at a much lower rate. The rate of release of labeled fatty acids from the substrates by the microsomal phospholipase decreased with increasing degree of unsaturation of the fatty acids at the 1 position. The presence of plasmalogens and of alkylacyl analogues in the incubation mixture caused an appreciable inhibition of the hydrolysis of the diacyl glycerophosphatides."}
{"id": "PMID:481685", "title": "[On the so-called \"hysteroepilepsy\"].", "content": "The authors examined on an outpatient basis 14 patients treated previously at the Neurological Department, Institute of Psychoneurology in Warsaw or at the Neurological Department of the Groch\u00f3w Hospital in the years 1965--1972 in whom epilepsy had been diagnosed but who had also had hysterical seizures. Young women aged 20--30 years with temporal-lobe epilepsy prevailed in this group. In most of them hysterical seizures appeared within 5 years after the first epileptic seizure when the frequency of true seizures decreased. In more than half these patients it was possible to find a connection between the occurrence of hypsterical seizures and conflict situations. Psychiatric examination failed to find in most cases any symptoms of epileptic characteropathy, but mood disturbances of the type of depression with dysphoria were disclosed, other findings included immature personality, egocentrism and extraversion. Although the clinical material reviewed by the authors contained only a negligible proportion of cases, it may be possible that they are more numerous among outpatients and the correct diagnosis of these cases is particularly important for further treatment since anticonvulsants are insufficient and sociotherapy with psychotherapy are indicated.", "contents": "[On the so-called \"hysteroepilepsy\"]. The authors examined on an outpatient basis 14 patients treated previously at the Neurological Department, Institute of Psychoneurology in Warsaw or at the Neurological Department of the Groch\u00f3w Hospital in the years 1965--1972 in whom epilepsy had been diagnosed but who had also had hysterical seizures. Young women aged 20--30 years with temporal-lobe epilepsy prevailed in this group. In most of them hysterical seizures appeared within 5 years after the first epileptic seizure when the frequency of true seizures decreased. In more than half these patients it was possible to find a connection between the occurrence of hypsterical seizures and conflict situations. Psychiatric examination failed to find in most cases any symptoms of epileptic characteropathy, but mood disturbances of the type of depression with dysphoria were disclosed, other findings included immature personality, egocentrism and extraversion. Although the clinical material reviewed by the authors contained only a negligible proportion of cases, it may be possible that they are more numerous among outpatients and the correct diagnosis of these cases is particularly important for further treatment since anticonvulsants are insufficient and sociotherapy with psychotherapy are indicated."}
{"id": "PMID:481686", "title": "[Psychomotor development of small children with epileptic attacks evaluated by a psychologist].", "content": "The reported investigations aimed at tracing the psychomoter development in small children with central nervous system damage for establishing whether there is any difference in the development of children in whom epileptic seizures developed as a manifestation of damage, in relation to children with palsies and epileptic seizures. The group of children observed comprised 46 cases aged from 1 to 3 years in two equal subgroups: children with epilepsy with maximal seizures and children with the diagnosis of cerebral infantile palsy with spastic quadriparesis and maximal epileptic seizures. The psychological investigations included clinical observation and experimental tests. The obtained results were subjected to analysis by the case study method. It was found that psychomotor development disturbances were more frequent in the group with cerebral palsy and epileptic seizures than in the group with epilepsy caused by brain damage. However, the level of psychomotor development of the observed children was not determined by palsy but the degree of damage to the central nervous system.", "contents": "[Psychomotor development of small children with epileptic attacks evaluated by a psychologist]. The reported investigations aimed at tracing the psychomoter development in small children with central nervous system damage for establishing whether there is any difference in the development of children in whom epileptic seizures developed as a manifestation of damage, in relation to children with palsies and epileptic seizures. The group of children observed comprised 46 cases aged from 1 to 3 years in two equal subgroups: children with epilepsy with maximal seizures and children with the diagnosis of cerebral infantile palsy with spastic quadriparesis and maximal epileptic seizures. The psychological investigations included clinical observation and experimental tests. The obtained results were subjected to analysis by the case study method. It was found that psychomotor development disturbances were more frequent in the group with cerebral palsy and epileptic seizures than in the group with epilepsy caused by brain damage. However, the level of psychomotor development of the observed children was not determined by palsy but the degree of damage to the central nervous system."}
{"id": "PMID:481687", "title": "[Xylose test in patients with attacks of headaches (migraine, Horton's headache)].", "content": "The aim of this work was to study digestive tract function by means of xylose test in patients with attacks of headaches. The investigations were carried out in 14 patients with migraine, 10 with Horton's headache and 1 patient with both these conditions. In patients with migraine or Horton's headache the xylose test was normal in 65% of cases, in the remaining 35% (8 patients, 4 in each group) xylose elimination was abnormal, because it was below 30%. The authors discuss the factors which could influence the abnormal results of xylose test in these patients, since they had no signs of other diseases than the mentioned headaches.", "contents": "[Xylose test in patients with attacks of headaches (migraine, Horton's headache)]. The aim of this work was to study digestive tract function by means of xylose test in patients with attacks of headaches. The investigations were carried out in 14 patients with migraine, 10 with Horton's headache and 1 patient with both these conditions. In patients with migraine or Horton's headache the xylose test was normal in 65% of cases, in the remaining 35% (8 patients, 4 in each group) xylose elimination was abnormal, because it was below 30%. The authors discuss the factors which could influence the abnormal results of xylose test in these patients, since they had no signs of other diseases than the mentioned headaches."}
{"id": "PMID:481688", "title": "[Encephalopathy after poisoning with organic mercury compounds].", "content": "Organic mercury compounds have greatest affinity to the central nervous system (cerebellar white matter, basal ganglia, occipital and frontal cortex). Anatomical changes observed in the brain are of degenerative type. Similar changes are found also in the myocardium, liver and kidneys. The authors observed for 2 years a family of three persons who contracted poisoning with organic mercury compounds after easting the meat of pigs and hens fed through negligence with wheat destined for sowing, containing methyl-mercurocyanoguanidine. The level of total mercury determined by the method of cold atomic absorption was in these patients four months after poisoning 650,500 and 175 ng/ml of blood (normal value from 1.56 to 18.72 ng/ml). During the observation it has been established that the severity of poisoning was related to the level of mercury found in the organism. Pharmacological treatment (Cuprenil, Thioctacid, vitamin B complex) and rehabilitation brough slight improvement after 2 years. Two patients with total blood mercury level 650 and 500 ng/ml were completely disabled due to encephalopathy with high grade ataxia, dysarthria and concentric narrowing of the field of vision. In the third patients with less severe poisoning (175 ng/ml) pathological manifestations disappeared completely after short treatment.", "contents": "[Encephalopathy after poisoning with organic mercury compounds]. Organic mercury compounds have greatest affinity to the central nervous system (cerebellar white matter, basal ganglia, occipital and frontal cortex). Anatomical changes observed in the brain are of degenerative type. Similar changes are found also in the myocardium, liver and kidneys. The authors observed for 2 years a family of three persons who contracted poisoning with organic mercury compounds after easting the meat of pigs and hens fed through negligence with wheat destined for sowing, containing methyl-mercurocyanoguanidine. The level of total mercury determined by the method of cold atomic absorption was in these patients four months after poisoning 650,500 and 175 ng/ml of blood (normal value from 1.56 to 18.72 ng/ml). During the observation it has been established that the severity of poisoning was related to the level of mercury found in the organism. Pharmacological treatment (Cuprenil, Thioctacid, vitamin B complex) and rehabilitation brough slight improvement after 2 years. Two patients with total blood mercury level 650 and 500 ng/ml were completely disabled due to encephalopathy with high grade ataxia, dysarthria and concentric narrowing of the field of vision. In the third patients with less severe poisoning (175 ng/ml) pathological manifestations disappeared completely after short treatment."}
{"id": "PMID:481689", "title": "[Clinical analysis of late infantile form of muscular atrophy].", "content": "Clinical analysis of 47 cases of late infantile spinal muscular atrophy is reported. In 25.5% of cases mothers reported weak fetal movements in pregnancy and low muscular tonus after birth, these signs predicted early manifestation of the disease. In 89,2% of cases the first signs of the disease appeared within the first 3 years of life. Delayed motor development in infancy was observed in 59.6%. Immobilization developed between the ages of 2 and 18 years, usually after 7 years of disease duration, and it was not correlated with the age of onset of the first signs of the disease. Mental development was normal in 81.5% of cases. Familial character of the disease was found in 51.1% of cases and in some of them intrafamilial variability was observed.", "contents": "[Clinical analysis of late infantile form of muscular atrophy]. Clinical analysis of 47 cases of late infantile spinal muscular atrophy is reported. In 25.5% of cases mothers reported weak fetal movements in pregnancy and low muscular tonus after birth, these signs predicted early manifestation of the disease. In 89,2% of cases the first signs of the disease appeared within the first 3 years of life. Delayed motor development in infancy was observed in 59.6%. Immobilization developed between the ages of 2 and 18 years, usually after 7 years of disease duration, and it was not correlated with the age of onset of the first signs of the disease. Mental development was normal in 81.5% of cases. Familial character of the disease was found in 51.1% of cases and in some of them intrafamilial variability was observed."}
{"id": "PMID:481690", "title": "[AMP-aminohydrolase of skeletal muscles in muscular diseases].", "content": "Determinations of AMP-aminohydrolase activity were done in muscles in 34 cases of different neuromuscular diseases, and 30 subjects without skeletal muscle changes. In the group of patients Duchenne's progressive dystrophy accounted for 10 cases, the remaining patients had limb-girdle dystrophy, polymyositis, myotonia, collagen diseases, myopathies of nuclear aetiology, and neurogenic muscle disease. The highest fall in the activity of the enzyme was observed in Duchenne's dystrophy. The authors discuss the possible mechanism of development of these abnormalities.", "contents": "[AMP-aminohydrolase of skeletal muscles in muscular diseases]. Determinations of AMP-aminohydrolase activity were done in muscles in 34 cases of different neuromuscular diseases, and 30 subjects without skeletal muscle changes. In the group of patients Duchenne's progressive dystrophy accounted for 10 cases, the remaining patients had limb-girdle dystrophy, polymyositis, myotonia, collagen diseases, myopathies of nuclear aetiology, and neurogenic muscle disease. The highest fall in the activity of the enzyme was observed in Duchenne's dystrophy. The authors discuss the possible mechanism of development of these abnormalities."}
{"id": "PMID:481691", "title": "[Effect of various forms of physical exertion on selected types of sensory function].", "content": "The author reports changes in vibration and two-point discrimation sensitivity in groups of healthy young males after several days of intense physical effort and after one short-lasting intense exercise. The vibration sensitivity was a steady type of sensory function showing no significant changes after these workloads. The two-point discrimination sensitivity was, not infrequently, better after several days of effort in relation to preliminary state, and it was regarded as an evidence of adaptation to work. On the other hand, one sort-lasting exercise reduced significantly discrimination of two stimuli, especially on the lower extremities. This significant impairment of two-stimuli discrimination on the lower extremities in relation to the upper ones after intensive effort suggests easier exhaustion of pathways conveying sensation in the thoracic segment of the spinal cord. On the basis of these investigations the author thinks that aesthesiometric investigations carried out under strictly observed conditions may be useful for assessment of fatigue.", "contents": "[Effect of various forms of physical exertion on selected types of sensory function]. The author reports changes in vibration and two-point discrimation sensitivity in groups of healthy young males after several days of intense physical effort and after one short-lasting intense exercise. The vibration sensitivity was a steady type of sensory function showing no significant changes after these workloads. The two-point discrimination sensitivity was, not infrequently, better after several days of effort in relation to preliminary state, and it was regarded as an evidence of adaptation to work. On the other hand, one sort-lasting exercise reduced significantly discrimination of two stimuli, especially on the lower extremities. This significant impairment of two-stimuli discrimination on the lower extremities in relation to the upper ones after intensive effort suggests easier exhaustion of pathways conveying sensation in the thoracic segment of the spinal cord. On the basis of these investigations the author thinks that aesthesiometric investigations carried out under strictly observed conditions may be useful for assessment of fatigue."}
{"id": "PMID:481692", "title": "[Favorable effect of procaine administration in retrobulbar optic neuritis].", "content": "The author reports 12 cases of retrobulbar neuritis of recent onset in which intravenous procaine exerted unexpectedly an immediate favourable effect. The treatment consisted of intravenous drip infusion of 40 ml of 1% procaine in 400 ml of 5% glucose during 7 days. The author supposes that the therapeutic effect was due to the antiallgeric action of procaine (blocking of histaminergic receptors) and its spasmolytic influence.", "contents": "[Favorable effect of procaine administration in retrobulbar optic neuritis]. The author reports 12 cases of retrobulbar neuritis of recent onset in which intravenous procaine exerted unexpectedly an immediate favourable effect. The treatment consisted of intravenous drip infusion of 40 ml of 1% procaine in 400 ml of 5% glucose during 7 days. The author supposes that the therapeutic effect was due to the antiallgeric action of procaine (blocking of histaminergic receptors) and its spasmolytic influence."}
{"id": "PMID:481693", "title": "[Place and role of clinical neurology in the opinion of students graduating from medical schools].", "content": "The attitudes and opinions of students of the 6th year of the medical faculties of four medical academies in Poland were studied. The proportion of students mentioning intention of specializing in neurology was 4--8%. Differences found in different medical academies between the attitude and opinions of students about the place and role of neurology among other specialities may be regarded as indicating the necessity of teaching modifications for stressing the importance of neurology in modern medicine and, especially, for emphasizing the consultative character of this speciality.", "contents": "[Place and role of clinical neurology in the opinion of students graduating from medical schools]. The attitudes and opinions of students of the 6th year of the medical faculties of four medical academies in Poland were studied. The proportion of students mentioning intention of specializing in neurology was 4--8%. Differences found in different medical academies between the attitude and opinions of students about the place and role of neurology among other specialities may be regarded as indicating the necessity of teaching modifications for stressing the importance of neurology in modern medicine and, especially, for emphasizing the consultative character of this speciality."}
{"id": "PMID:481694", "title": "[Rare care of Ramsay Hunt syndrome associated with herpes zoster of the glossopharyngeal nerve].", "content": "The authors describe a rare coexistence of Ramsay Hunt syndrome with glossopharyngeal zoster in a 67-year-old patient. A trial of systematization of the nomenclature of Ramsay Hunt syndrome is suggested, on the basis of certain anatomophysiological data. It is concluded that in zoster involvement of the sensory elements in the geniculate ganglion and in otic ganglion should be accompanied by taste sensitivity disturbances, but often the patients fail to notice these disturbances and routine taste testing shows also no such tase impairment. Routine use of electrogustometry is postulated since this makes possible diagnostic-prognostic assessment followed by selection of appropriate treatment.", "contents": "[Rare care of Ramsay Hunt syndrome associated with herpes zoster of the glossopharyngeal nerve]. The authors describe a rare coexistence of Ramsay Hunt syndrome with glossopharyngeal zoster in a 67-year-old patient. A trial of systematization of the nomenclature of Ramsay Hunt syndrome is suggested, on the basis of certain anatomophysiological data. It is concluded that in zoster involvement of the sensory elements in the geniculate ganglion and in otic ganglion should be accompanied by taste sensitivity disturbances, but often the patients fail to notice these disturbances and routine taste testing shows also no such tase impairment. Routine use of electrogustometry is postulated since this makes possible diagnostic-prognostic assessment followed by selection of appropriate treatment."}
{"id": "PMID:481699", "title": "The density of neurons in the human hippocampus.", "content": "The aim of this study was to determine the density and pattern of distribution of the neurons in the human hippocampus, and twenty-nine brains of healthy individuals were examined. The cells counted in each unit area were the pyramidal cells in the H-fields and the granule cells. Difference in density was found between all H-fields. The neuronal density was highest in the H2-fields. The density decreased through the transitional area H1-2 and H1, with the lowest density in H3. The neuronal density increased in all H-fields in the antero-posterior direction, while the granule cells decreased in density. In the individual brain there was a positive correlation found respectively between high and low cell density in the different H-fields. The pyramidal cell density decreased in persons more than 68 years old. No difference was found between neruonal cell density in the two sides of the brain. Sex did not influence the neuronal cell density.", "contents": "The density of neurons in the human hippocampus. The aim of this study was to determine the density and pattern of distribution of the neurons in the human hippocampus, and twenty-nine brains of healthy individuals were examined. The cells counted in each unit area were the pyramidal cells in the H-fields and the granule cells. Difference in density was found between all H-fields. The neuronal density was highest in the H2-fields. The density decreased through the transitional area H1-2 and H1, with the lowest density in H3. The neuronal density increased in all H-fields in the antero-posterior direction, while the granule cells decreased in density. In the individual brain there was a positive correlation found respectively between high and low cell density in the different H-fields. The pyramidal cell density decreased in persons more than 68 years old. No difference was found between neruonal cell density in the two sides of the brain. Sex did not influence the neuronal cell density."}
{"id": "PMID:481695", "title": "[Transient focal ischemia in Fahr's disease].", "content": "In the reported case symmetrical calcifications were found in the basal ganglia and cerebellum which connected with congenital anomalies of cerebral vessels were the cause of intermittent episodes of right-sided hemiparesis with mutism in a man aged 39 years.", "contents": "[Transient focal ischemia in Fahr's disease]. In the reported case symmetrical calcifications were found in the basal ganglia and cerebellum which connected with congenital anomalies of cerebral vessels were the cause of intermittent episodes of right-sided hemiparesis with mutism in a man aged 39 years."}
{"id": "PMID:481700", "title": "The fine structure of the cns in multiple sclerosis. II. Vesilcular demyelination in an acute case.", "content": "Tubular vesicular and net-like dissolution of myelin sheaths associated with complete demyelination and preservation of axons, is described in the brain, obtained within 4 h of death, from a patient who died with acute multiple sclerosis (MS). It was rare, being found in only three out of twenty-three blocks examined, and was seen mainly in the partially demyelinated margin of an active plaque in the white matter. The possibility that post-mortem autolytic changes or fixation artefact might have accounted for the appearances is considered, but is thought unlikely. The findings, though not specific would be expected if current theories invoking myelin-directed 'autoimmune' mechanisms in MS pathogenesis are correct. However, other myelin-directed mechanisms cannot be excluded on the basis of this evidence alone.", "contents": "The fine structure of the cns in multiple sclerosis. II. Vesilcular demyelination in an acute case. Tubular vesicular and net-like dissolution of myelin sheaths associated with complete demyelination and preservation of axons, is described in the brain, obtained within 4 h of death, from a patient who died with acute multiple sclerosis (MS). It was rare, being found in only three out of twenty-three blocks examined, and was seen mainly in the partially demyelinated margin of an active plaque in the white matter. The possibility that post-mortem autolytic changes or fixation artefact might have accounted for the appearances is considered, but is thought unlikely. The findings, though not specific would be expected if current theories invoking myelin-directed 'autoimmune' mechanisms in MS pathogenesis are correct. However, other myelin-directed mechanisms cannot be excluded on the basis of this evidence alone."}
{"id": "PMID:481697", "title": "[Simultaneous occurrence of meningioma and astrocytoma].", "content": "The authors present a case of a 47 year-old woman admitted to Neurosurgical Clinic with diagnosis of left frontal lobe tumour. At operation, during removal of glioma of the frontal lobe, a parasagital meningioma, 3 cm in diameter, was found and removed. Both tumours were adjacent. Histopatologic examination revealed: grade 1 astrocytoma and fibroblastic meningioma. Some clinical and pathological problems associated with simultaneous occurrence of two tumours of various histologic origin are discussed.", "contents": "[Simultaneous occurrence of meningioma and astrocytoma]. The authors present a case of a 47 year-old woman admitted to Neurosurgical Clinic with diagnosis of left frontal lobe tumour. At operation, during removal of glioma of the frontal lobe, a parasagital meningioma, 3 cm in diameter, was found and removed. Both tumours were adjacent. Histopatologic examination revealed: grade 1 astrocytoma and fibroblastic meningioma. Some clinical and pathological problems associated with simultaneous occurrence of two tumours of various histologic origin are discussed."}
{"id": "PMID:481696", "title": "[Medulloblastoma with multiple metastases beyond the nervous system].", "content": "A case of desmoplastic cerebellar medulloblastoma with numerous metastases beyond the nervous system is reported. Fourteen months after the operation the patient began complaining about pains in hip and knee joints and dysphagia. One week before death profuse bleeding from the digestive tract developed. On autopsy numerous metastases were found in the bones, lymph nodes, spleen, pancreas and kidneys. Histological examination demonstrated that the metastases were composed of the same cells as the primary cerebellar tumour, but reticulin fibres were scant in them. In none of the metastatic lesions any significant reticulin proliferation was present, these fibres were found only around small vessels.", "contents": "[Medulloblastoma with multiple metastases beyond the nervous system]. A case of desmoplastic cerebellar medulloblastoma with numerous metastases beyond the nervous system is reported. Fourteen months after the operation the patient began complaining about pains in hip and knee joints and dysphagia. One week before death profuse bleeding from the digestive tract developed. On autopsy numerous metastases were found in the bones, lymph nodes, spleen, pancreas and kidneys. Histological examination demonstrated that the metastases were composed of the same cells as the primary cerebellar tumour, but reticulin fibres were scant in them. In none of the metastatic lesions any significant reticulin proliferation was present, these fibres were found only around small vessels."}
{"id": "PMID:481702", "title": "A quantitative study of cell proliferation in ependyma and choroid plexus in the postnatal rat brain.", "content": "Profliferative activity in forebrain ependyma aand choroid plexus epithelium was studied in rats aged up to 42 days by means of autoradiography after injection of [3H]-thymidine. Labelling indices were higher in the lateral than in the third ventricle, while values for choroid plexus epithelium were lower than those for ependyma. In all areas examined, labelling indices showed a similar pattern of decline in the first two postnatal weeks reaching by day 12 a level about 20-30% of that on day 1. This low level, corresponding to a turnover time of 130 days for ependyma, was maintained tereafter up to day 42, indicating the persistence of proliferation in the young adult.", "contents": "A quantitative study of cell proliferation in ependyma and choroid plexus in the postnatal rat brain. Profliferative activity in forebrain ependyma aand choroid plexus epithelium was studied in rats aged up to 42 days by means of autoradiography after injection of [3H]-thymidine. Labelling indices were higher in the lateral than in the third ventricle, while values for choroid plexus epithelium were lower than those for ependyma. In all areas examined, labelling indices showed a similar pattern of decline in the first two postnatal weeks reaching by day 12 a level about 20-30% of that on day 1. This low level, corresponding to a turnover time of 130 days for ependyma, was maintained tereafter up to day 42, indicating the persistence of proliferation in the young adult."}
{"id": "PMID:481698", "title": "[Meningomyelocele with spinal cord cyst and internal hydrocephalus in a 9-month-old boy].", "content": "The authors describe a boy aged 9 months with open myelomeningocele which changed gradually into meningocele with spinal cord cyst. During treatment internal hydrocephalus developed requiring insertion of Pudenz-Heyer ventriculoatrial.  valve. The treatment was successful. The case illustrates the succession of changes which may develop in myelomeningocele and the relationship between the state of internal hydrocephalus and the volume of meningocele and spinal cyst (cerebrospinal fluid production exceeding its resorption).", "contents": "[Meningomyelocele with spinal cord cyst and internal hydrocephalus in a 9-month-old boy]. The authors describe a boy aged 9 months with open myelomeningocele which changed gradually into meningocele with spinal cord cyst. During treatment internal hydrocephalus developed requiring insertion of Pudenz-Heyer ventriculoatrial.  valve. The treatment was successful. The case illustrates the succession of changes which may develop in myelomeningocele and the relationship between the state of internal hydrocephalus and the volume of meningocele and spinal cyst (cerebrospinal fluid production exceeding its resorption)."}
{"id": "PMID:481711", "title": "Intracranial pressure time course in primary intracerebral hemorrhage.", "content": "The course of intracranial pressure (ICP) over time was studied in 66 hypertensive and/or altherosclerotic patients harboring intracerebral hematomas. Patients with no disturbance of consciousness showed normal or only slightly elevated pressure. Conversely, most patients in deep coma exhibited high pressure with a tendency to rise further no matter what treatment was used. In the remaining patients with intermediate disturbances of consciousness, no definite correlation was found between ICP, clinical condition, and outcome. In all of the patients who underwent operation, the postoperative course of ICP over time was also studied and seemed to depend to a certain extent on the timing of the operation.", "contents": "Intracranial pressure time course in primary intracerebral hemorrhage. The course of intracranial pressure (ICP) over time was studied in 66 hypertensive and/or altherosclerotic patients harboring intracerebral hematomas. Patients with no disturbance of consciousness showed normal or only slightly elevated pressure. Conversely, most patients in deep coma exhibited high pressure with a tendency to rise further no matter what treatment was used. In the remaining patients with intermediate disturbances of consciousness, no definite correlation was found between ICP, clinical condition, and outcome. In all of the patients who underwent operation, the postoperative course of ICP over time was also studied and seemed to depend to a certain extent on the timing of the operation."}
{"id": "PMID:481713", "title": "Visual evoked potentials: a noninvasive technique to monitor patients with shunted hydrocephalus.", "content": "Visual evoked potentials (VEPs) to repetitive flash stimuli were abnormal in 10 patients with documented hydrocephalus. Abnormalities included latency delays, fatigability, and asymmetries. Both latency and wave form disturbances improved in the postshunt period. Clinical progression of the hydrocephalus occurred in several patients, and this correlated well with worsening of the VEPs. We suggest that this noninvasive technique may be valuable in following patients with cerebrospinal fluid shunts, patients with presumed \"arrested\" hydrocephalus, and patients who are being weaned from their shunts.", "contents": "Visual evoked potentials: a noninvasive technique to monitor patients with shunted hydrocephalus. Visual evoked potentials (VEPs) to repetitive flash stimuli were abnormal in 10 patients with documented hydrocephalus. Abnormalities included latency delays, fatigability, and asymmetries. Both latency and wave form disturbances improved in the postshunt period. Clinical progression of the hydrocephalus occurred in several patients, and this correlated well with worsening of the VEPs. We suggest that this noninvasive technique may be valuable in following patients with cerebrospinal fluid shunts, patients with presumed \"arrested\" hydrocephalus, and patients who are being weaned from their shunts."}
{"id": "PMID:481714", "title": "Cerebrospinal fluid bulk flow study: radioiodinated serum albumin (R131ISA) and technetium-99m-labeled diethylenetriaminepentaacetic acid (DTPA) retention in the ventricles of normal and hydrocephalic dogs.", "content": "Eight normal dogs were studied and then made hydrocephalic (kaolin induced) to demonstrate and compare the use of R131ISA and 99mTcDTPA in cerebrospinal fluid (CSF) bulk flow studies. The radioisotopes were simultaneously injected into the CSF space in the cisterna magna of normal dogs and into the lateral ventricles of hydrocephalic dogs after an equal volume of CSF had been removed. The average dose of RISA was 100 muCi, and for TcDTPA it was 7 mCi. A bag of lactated Ringer's solution (500 ml) was used as a control phantom after being injected with the same dose of isotope as used in the animals. Brain scans were obtained with the gamma ray camera at 4 and 24 hours after injection. We used four criteria to evaluate results: clinical state of the animal, CSF ventricular pressures with challenges, percentage of retained isotope in counts per minute, and ventricular size at postmortem examination. The results demonstrate that the amount of isotope retention over 20 hours and the clinical state of the dog are better correlated when RISA is used than when TcDTPA is used. The study indicates that RISA is more reliable than TcDTPA as an indicator of CSF bulk flow in both normal and hydrocephalic dogs.", "contents": "Cerebrospinal fluid bulk flow study: radioiodinated serum albumin (R131ISA) and technetium-99m-labeled diethylenetriaminepentaacetic acid (DTPA) retention in the ventricles of normal and hydrocephalic dogs. Eight normal dogs were studied and then made hydrocephalic (kaolin induced) to demonstrate and compare the use of R131ISA and 99mTcDTPA in cerebrospinal fluid (CSF) bulk flow studies. The radioisotopes were simultaneously injected into the CSF space in the cisterna magna of normal dogs and into the lateral ventricles of hydrocephalic dogs after an equal volume of CSF had been removed. The average dose of RISA was 100 muCi, and for TcDTPA it was 7 mCi. A bag of lactated Ringer's solution (500 ml) was used as a control phantom after being injected with the same dose of isotope as used in the animals. Brain scans were obtained with the gamma ray camera at 4 and 24 hours after injection. We used four criteria to evaluate results: clinical state of the animal, CSF ventricular pressures with challenges, percentage of retained isotope in counts per minute, and ventricular size at postmortem examination. The results demonstrate that the amount of isotope retention over 20 hours and the clinical state of the dog are better correlated when RISA is used than when TcDTPA is used. The study indicates that RISA is more reliable than TcDTPA as an indicator of CSF bulk flow in both normal and hydrocephalic dogs."}
{"id": "PMID:481715", "title": "Time course of serotonin-induced vasoconstriction.", "content": "Canine cerebral arterial segments tested in a tissue bath were found to escape from the vasoconstrictor effects of serotonin, the basilar segments more rapidly than the middle cerebral segments. At low doses (10(-9) M), serotonin caused a small, prolonged contraction, whereas at higher doses (10(-6) M), a more forceful phasic response was seen. Verapamil blocked the phasic portion of the response but not the low amplitude sustained portion. Increasing the bath concentration of K+, Ba++, and tetraethylammonium markedly enhanced the constrictor response of low serotonin doses and significantly inhibited escape. Potentiation of the force and duration of vasoactive amine-induced arterial constriction by small increments of extracellular K+ might be important in the prolonged vascular narrowing associated with subarachnoid hemorrhage.", "contents": "Time course of serotonin-induced vasoconstriction. Canine cerebral arterial segments tested in a tissue bath were found to escape from the vasoconstrictor effects of serotonin, the basilar segments more rapidly than the middle cerebral segments. At low doses (10(-9) M), serotonin caused a small, prolonged contraction, whereas at higher doses (10(-6) M), a more forceful phasic response was seen. Verapamil blocked the phasic portion of the response but not the low amplitude sustained portion. Increasing the bath concentration of K+, Ba++, and tetraethylammonium markedly enhanced the constrictor response of low serotonin doses and significantly inhibited escape. Potentiation of the force and duration of vasoactive amine-induced arterial constriction by small increments of extracellular K+ might be important in the prolonged vascular narrowing associated with subarachnoid hemorrhage."}
{"id": "PMID:481718", "title": "Ten years of national health service in Canada.", "content": "Ten years ago the medical profession in Canada was conscripted into a compulsory national health insurance scheme without any prior consultation. Predictions that an open-ended, so-called \"free\" medical insurance scheme would lead to cost overruns and deterioration of medical services as well as inflationary trends have come true. Federal and provincial governments now attempt to contain costs by closing active treatment beds or filling them up with chronic patients and by attrition of existing facilities. Compulsory national health insurance has not led to lower morbidity nor greater longevity. It has only provoked an insatiable demand from the public for more \"free\" services, with the result that the system has become a quagmire of cost overruns and unfulfilled and unrealizable promises.", "contents": "Ten years of national health service in Canada. Ten years ago the medical profession in Canada was conscripted into a compulsory national health insurance scheme without any prior consultation. Predictions that an open-ended, so-called \"free\" medical insurance scheme would lead to cost overruns and deterioration of medical services as well as inflationary trends have come true. Federal and provincial governments now attempt to contain costs by closing active treatment beds or filling them up with chronic patients and by attrition of existing facilities. Compulsory national health insurance has not led to lower morbidity nor greater longevity. It has only provoked an insatiable demand from the public for more \"free\" services, with the result that the system has become a quagmire of cost overruns and unfulfilled and unrealizable promises."}
{"id": "PMID:481725", "title": "Blunt trauma to the high cervical carotid artery.", "content": "Closed injuries to the neck, occasionally associated with mandibular fractures, can result in trauma to the internal carotid artery at the high cervical level opposite the uppermost cervical vertebrae. Such arterial injuries range from spasm to intimal tear with thrombus formation, medial tear, and aneurysm formation. These types of lesions may overlap. Ten patients are presented, five with traumatic carotid occlusions and five with traumatic aneurysms. The clinical diagnosis of traumatic cervical carotid occlusion may be difficult because of the absence of physical trauma to the neck. The diagnosis of aneurysm poses fewer problems. Oculopneumoplethysmography, computerized tomographic head scanning, and carotid arteriography with cross compression are the most useful diagnostic techniques. Thrombectomy should be carried out early, particularly in the presence of fluctuating neurological signs. Aneurysms that cannot be approached directly may necessitate carotid ligation.", "contents": "Blunt trauma to the high cervical carotid artery. Closed injuries to the neck, occasionally associated with mandibular fractures, can result in trauma to the internal carotid artery at the high cervical level opposite the uppermost cervical vertebrae. Such arterial injuries range from spasm to intimal tear with thrombus formation, medial tear, and aneurysm formation. These types of lesions may overlap. Ten patients are presented, five with traumatic carotid occlusions and five with traumatic aneurysms. The clinical diagnosis of traumatic cervical carotid occlusion may be difficult because of the absence of physical trauma to the neck. The diagnosis of aneurysm poses fewer problems. Oculopneumoplethysmography, computerized tomographic head scanning, and carotid arteriography with cross compression are the most useful diagnostic techniques. Thrombectomy should be carried out early, particularly in the presence of fluctuating neurological signs. Aneurysms that cannot be approached directly may necessitate carotid ligation."}
{"id": "PMID:481726", "title": "Radionuclide cerebral angiography and the timing of aneurysm surgery.", "content": "Forty-five patients with subarachnoid hemorrhage due to verified intracranial aneurysms were studied prospectively to determine whether delaying operations in those patients with abnormal cerebral perfusion, assessed by radionuclide dynamic scanning, would lower case management mortality. Twenty-nine patients had intracranial operations when their radionuclide dynamic scans demonstrated normal perfusion. The one death in this group occurred in a patient who suffered a massive hemorrhage during operation as the bone flap was elevated. There were no instances of delayed spasm after operation. Of the 16 remaining patients who were treated without operation, 5 died. Only 1 of these deaths resulted from recurrent hemorrhage. This patient had normal cerebral perfusion but was not operated upon because of severe associated medical problems. Cerebral infarction occurred in 10 of the 16 unoperated patients, all of whom had persistently decreased cerebral perfusion. Four of these patients died of their infarctions or related causes. Although a trend related vessel caliber as seen on arteriograms and perfusion delay seen on dynamic scanning, individual exceptions were numerous. This study shows that radionuclide scanning, a safe procedure, is useful for identifying two types of patients: those with normal perfusion prone to rebleeding in whom an early operation is safe and those in whom perfusion is decreased. The latter group is prone to cerebral infarction, but rebleeding from the aneurysm is unlikely. Operation should be delayed in these patients until cerebral perfusion returns to normal or for several weeks if cerebral perfusion remains diminished.", "contents": "Radionuclide cerebral angiography and the timing of aneurysm surgery. Forty-five patients with subarachnoid hemorrhage due to verified intracranial aneurysms were studied prospectively to determine whether delaying operations in those patients with abnormal cerebral perfusion, assessed by radionuclide dynamic scanning, would lower case management mortality. Twenty-nine patients had intracranial operations when their radionuclide dynamic scans demonstrated normal perfusion. The one death in this group occurred in a patient who suffered a massive hemorrhage during operation as the bone flap was elevated. There were no instances of delayed spasm after operation. Of the 16 remaining patients who were treated without operation, 5 died. Only 1 of these deaths resulted from recurrent hemorrhage. This patient had normal cerebral perfusion but was not operated upon because of severe associated medical problems. Cerebral infarction occurred in 10 of the 16 unoperated patients, all of whom had persistently decreased cerebral perfusion. Four of these patients died of their infarctions or related causes. Although a trend related vessel caliber as seen on arteriograms and perfusion delay seen on dynamic scanning, individual exceptions were numerous. This study shows that radionuclide scanning, a safe procedure, is useful for identifying two types of patients: those with normal perfusion prone to rebleeding in whom an early operation is safe and those in whom perfusion is decreased. The latter group is prone to cerebral infarction, but rebleeding from the aneurysm is unlikely. Operation should be delayed in these patients until cerebral perfusion returns to normal or for several weeks if cerebral perfusion remains diminished."}
{"id": "PMID:481727", "title": "Computerized tomographic diagnosis: pitfalls for neurosurgeons.", "content": "Computerized tomography as a diagnostic procedure is characterized by its high sensitivity but limited specificity. This lack of specificity may result in an erroneous diagnosis and, possibly, in ill-advised therapy unless clinical and objective data are carefully considered in interpreting the study. The most common pitfalls encountered are the phenomena of contrast enhancement of infarcts and mass effect associated with infarcts. Many different pathological processes can have similar enhancement patterns. The evolution of high density, acute hemorrhage to isodense areas in the chronic hematoma can become a serious diagnostic problem unless an appropriate history is available. The authors discuss 30 cases in which misinterpretation of CT scans resulted in erroneous diagnoses. Seventeen of these patients underwent operation.", "contents": "Computerized tomographic diagnosis: pitfalls for neurosurgeons. Computerized tomography as a diagnostic procedure is characterized by its high sensitivity but limited specificity. This lack of specificity may result in an erroneous diagnosis and, possibly, in ill-advised therapy unless clinical and objective data are carefully considered in interpreting the study. The most common pitfalls encountered are the phenomena of contrast enhancement of infarcts and mass effect associated with infarcts. Many different pathological processes can have similar enhancement patterns. The evolution of high density, acute hemorrhage to isodense areas in the chronic hematoma can become a serious diagnostic problem unless an appropriate history is available. The authors discuss 30 cases in which misinterpretation of CT scans resulted in erroneous diagnoses. Seventeen of these patients underwent operation."}
{"id": "PMID:481728", "title": "The trapped temporal horn: a trap in neuroradiological diagnosis.", "content": "Although the advent of computerized cranial tomography (CT) has decreased the number of pneumoencephalograms performed for the diagnosis of hydrocephalus and lesions of the posterior fossa, brain stem, and ventricles, there are some patients in whom pneumoencephalography should still be done because it adds valuable information to that obtained with CT. When the temporal horn becomes obstructed, the choroid plexus and ependymal surface \"upstream\" from the obstructing mass continue to produce cerebrospinal fluid (CSF). The temporal horn can thus enlarge enough to appear as a mass on CT because of its reduced x-ray attenuation coefficient. Pneumoencephalography is effective in this situation because air will flow past a mass that obstructs CSF and because the ventricular system dilates during pneumoencephalography. When pneumoencephalography is used in a patient with a trapped temporal horn, the partially trapped horn may enlarge approximately 24 hours later. With that precaution in mind, the neurosurgeon should find pneumoencephalography to be a useful adjunct to CT in delineating the cause of a trapped temporal horn. In the three patients reported here CT had indicated a unilateral trapped temporal horn; pneumoencephalography confirmed that finding and demonstrated both the location and the nature of the lesion. One patient had a Grade II astrocytoma fungating into the atrium of the right lateral ventricle, one had a mass extending into the right ventricle from the medial and superior ventricular wall with nodular encroachment on the ventricle, and one had a meningioma in the atrium of the right lateral ventricle.", "contents": "The trapped temporal horn: a trap in neuroradiological diagnosis. Although the advent of computerized cranial tomography (CT) has decreased the number of pneumoencephalograms performed for the diagnosis of hydrocephalus and lesions of the posterior fossa, brain stem, and ventricles, there are some patients in whom pneumoencephalography should still be done because it adds valuable information to that obtained with CT. When the temporal horn becomes obstructed, the choroid plexus and ependymal surface \"upstream\" from the obstructing mass continue to produce cerebrospinal fluid (CSF). The temporal horn can thus enlarge enough to appear as a mass on CT because of its reduced x-ray attenuation coefficient. Pneumoencephalography is effective in this situation because air will flow past a mass that obstructs CSF and because the ventricular system dilates during pneumoencephalography. When pneumoencephalography is used in a patient with a trapped temporal horn, the partially trapped horn may enlarge approximately 24 hours later. With that precaution in mind, the neurosurgeon should find pneumoencephalography to be a useful adjunct to CT in delineating the cause of a trapped temporal horn. In the three patients reported here CT had indicated a unilateral trapped temporal horn; pneumoencephalography confirmed that finding and demonstrated both the location and the nature of the lesion. One patient had a Grade II astrocytoma fungating into the atrium of the right lateral ventricle, one had a mass extending into the right ventricle from the medial and superior ventricular wall with nodular encroachment on the ventricle, and one had a meningioma in the atrium of the right lateral ventricle."}
{"id": "PMID:481729", "title": "Fracture and complete dislocation of the thoracic or lumbosacral spine: report of three cases.", "content": "Severe fracture/dislocation of the thoracic (T) or lumbar (L) spine is usually associated with complete neurological dysfunction below the level of injury. Three cases of severe spinal fracture/dislocation are presented in this report. Two of these patients suffered only partial neurological deficit, which improved after open reduction, internal fixation, and bone fusion. Severe fracture/dislocation of the T or L spine may be quite obvious on lateral roentgenograms. Occasionally, however, the fracture site may be obscured by the overlying shoulders, hips, or soft tissues. If no lateral displacement is evident on anteroposterior (AP) films, a false impression of normal alignment may be given, as was the case in two of our patients. A careful interpretation of the AP view will provide the diagnosis in such cases. The subtle AP radiographic changes suggesting fracture/dislocation of the T or L spine are detailed. The mode of injury, physical findings, prognosis, and surgical treatment of such severe injuries are also briefly reviewed.", "contents": "Fracture and complete dislocation of the thoracic or lumbosacral spine: report of three cases. Severe fracture/dislocation of the thoracic (T) or lumbar (L) spine is usually associated with complete neurological dysfunction below the level of injury. Three cases of severe spinal fracture/dislocation are presented in this report. Two of these patients suffered only partial neurological deficit, which improved after open reduction, internal fixation, and bone fusion. Severe fracture/dislocation of the T or L spine may be quite obvious on lateral roentgenograms. Occasionally, however, the fracture site may be obscured by the overlying shoulders, hips, or soft tissues. If no lateral displacement is evident on anteroposterior (AP) films, a false impression of normal alignment may be given, as was the case in two of our patients. A careful interpretation of the AP view will provide the diagnosis in such cases. The subtle AP radiographic changes suggesting fracture/dislocation of the T or L spine are detailed. The mode of injury, physical findings, prognosis, and surgical treatment of such severe injuries are also briefly reviewed."}
{"id": "PMID:481731", "title": "Growth-inhibitory effect of diphenylhydantoin on murine astrocytomas.", "content": "In previous work reported from this laboratory we found that diphenylhydantoin (DPH) inhibited the growth of 7 of 10 tissue-cultured human astrocytoma cell lines in a microtiter system. In this report we describe significant growth inhibition by DPH of two murine astrocytoma tissue cultures and correlate these in vitro findings with the in vivo activity of DPH in rat subcutaneous and intracranial tumor models. In the in vivo studies, rats were inoculated either subcutaneously or intracranially with RT9 or C6 rat gliomas. DPH or placebo was injected intraperitoneally in doses ranging from 50 to 150 mg/kg daily for 15 days. The DPH-treated rats showed significantly slower rates of tumor growth than untreated rats (p less than 0.01) in both the subcutaneous and intracranial models. At sacrifice, the tumor volume of the rats with subcutaneous tumors treated with DPH (100 mg/kg daily) was 62% less than the tumor volume of the control rats. Also, the number of \"clonogenic\" cells and thus, indirectly, the number of actively dividing tumor cells was 54% less in the intracranial tumors of the DPH-treated rats. These findings indicate that DPH may be a potentially useful adjunctive agent in the clinical chemotherapy of astrocytic tumors. Furthermore, the data presented indicate that in vitro test results are predictive for in vivo effects, supporting the idea that tissue culture can be used to screen for the effectiveness of clinically used chemotherapeutic agents.", "contents": "Growth-inhibitory effect of diphenylhydantoin on murine astrocytomas. In previous work reported from this laboratory we found that diphenylhydantoin (DPH) inhibited the growth of 7 of 10 tissue-cultured human astrocytoma cell lines in a microtiter system. In this report we describe significant growth inhibition by DPH of two murine astrocytoma tissue cultures and correlate these in vitro findings with the in vivo activity of DPH in rat subcutaneous and intracranial tumor models. In the in vivo studies, rats were inoculated either subcutaneously or intracranially with RT9 or C6 rat gliomas. DPH or placebo was injected intraperitoneally in doses ranging from 50 to 150 mg/kg daily for 15 days. The DPH-treated rats showed significantly slower rates of tumor growth than untreated rats (p less than 0.01) in both the subcutaneous and intracranial models. At sacrifice, the tumor volume of the rats with subcutaneous tumors treated with DPH (100 mg/kg daily) was 62% less than the tumor volume of the control rats. Also, the number of \"clonogenic\" cells and thus, indirectly, the number of actively dividing tumor cells was 54% less in the intracranial tumors of the DPH-treated rats. These findings indicate that DPH may be a potentially useful adjunctive agent in the clinical chemotherapy of astrocytic tumors. Furthermore, the data presented indicate that in vitro test results are predictive for in vivo effects, supporting the idea that tissue culture can be used to screen for the effectiveness of clinically used chemotherapeutic agents."}
{"id": "PMID:481734", "title": "Carcinoma associated with lipomeningocele: case report.", "content": "We report the case of a 41-year-old man with an epidermoid carcinoma that had developed on a meningocele with a fistulous tract that chronically drained cerebrospinal fluid. After a review of the literature, we discuss the cause of this rare occurrence. We think that the recurrent discharge of spinal fluid (never complicated by meningitis) may have caused a chronic irritation of the tissues and then the delayed development of a carcinoma, which is similar to a case found in the literature. In our case there was a \"flow\" of carcinomatous cells along the fistula, which entered the meningocele, invading the subdural space as far as the peduncle. Our patient, who had a benign lesion (meningocele), came under neurosurgical care only after the lesion had degenerated. The malignant degeneration of such a benign lesion emphasizes the need for surgical treatment as soon as possible.", "contents": "Carcinoma associated with lipomeningocele: case report. We report the case of a 41-year-old man with an epidermoid carcinoma that had developed on a meningocele with a fistulous tract that chronically drained cerebrospinal fluid. After a review of the literature, we discuss the cause of this rare occurrence. We think that the recurrent discharge of spinal fluid (never complicated by meningitis) may have caused a chronic irritation of the tissues and then the delayed development of a carcinoma, which is similar to a case found in the literature. In our case there was a \"flow\" of carcinomatous cells along the fistula, which entered the meningocele, invading the subdural space as far as the peduncle. Our patient, who had a benign lesion (meningocele), came under neurosurgical care only after the lesion had degenerated. The malignant degeneration of such a benign lesion emphasizes the need for surgical treatment as soon as possible."}
{"id": "PMID:481738", "title": "Extravasation and arteriovenous shunting after epidural bleeding -- a radiological study.", "content": "Eighteen patients with epidural bleeding had preoperative angiography with visualization of the meningeal arteries. Thirteen of these had extravasation of contrast medium from meningeal arteries and eleven also had shunting of contrast medium from meningeal arteries to meningeal or diploic veins. For easier demonstration of these findings, selective external carotid angiography and the use of larger amounts of contrast medium are recommended. Our results support the theory that the arteriovenous shunting has important implications on the pathophysiology of epidural bleeding. Failure to demonstrate extravasation and arteriovenous shunting may be explained in three ways: (1) inadequate angiographic technique; (2) arterial bleeding has stopped; (3) epidural bleeding is not arterial.", "contents": "Extravasation and arteriovenous shunting after epidural bleeding -- a radiological study. Eighteen patients with epidural bleeding had preoperative angiography with visualization of the meningeal arteries. Thirteen of these had extravasation of contrast medium from meningeal arteries and eleven also had shunting of contrast medium from meningeal arteries to meningeal or diploic veins. For easier demonstration of these findings, selective external carotid angiography and the use of larger amounts of contrast medium are recommended. Our results support the theory that the arteriovenous shunting has important implications on the pathophysiology of epidural bleeding. Failure to demonstrate extravasation and arteriovenous shunting may be explained in three ways: (1) inadequate angiographic technique; (2) arterial bleeding has stopped; (3) epidural bleeding is not arterial."}
{"id": "PMID:481739", "title": "On the symmetry and asymmetry of the bifurcation of the common carotid artery: a study of bilateral carotid angiograms in 100 adults.", "content": "Bilateral carotid angiograms of the neck in 100 consecutive adult patients show the bifurcation of the left common carotid artery to be located cranial to the right in 50% of the cases, while the right bifurcation was higher in 22%. The origin of the internal carotid artery was at the dorsal or dorsolateral aspect of the common carotid artery in 82% on the right side and in 94% on the left, while a dorsomedial or medial origin was found in 18% on the right side and in 6% on the left.", "contents": "On the symmetry and asymmetry of the bifurcation of the common carotid artery: a study of bilateral carotid angiograms in 100 adults. Bilateral carotid angiograms of the neck in 100 consecutive adult patients show the bifurcation of the left common carotid artery to be located cranial to the right in 50% of the cases, while the right bifurcation was higher in 22%. The origin of the internal carotid artery was at the dorsal or dorsolateral aspect of the common carotid artery in 82% on the right side and in 94% on the left, while a dorsomedial or medial origin was found in 18% on the right side and in 6% on the left."}
{"id": "PMID:481740", "title": "Medical hypnosis during therapeutic embolizations of the carotid and vertebral arteries.", "content": "Iatrogenic neurological deficit constitutes the greatest risk during therapeutic embolization in the vascular territories of the carotid and vertebral arteries. Constant monitoring of the patient's neurological status is therefore essential. The authors discuss the use of medical hypnosis to circumvent general anesthesia and to reduce chemical sedation to a minimum. Their experience with this technique is presented.", "contents": "Medical hypnosis during therapeutic embolizations of the carotid and vertebral arteries. Iatrogenic neurological deficit constitutes the greatest risk during therapeutic embolization in the vascular territories of the carotid and vertebral arteries. Constant monitoring of the patient's neurological status is therefore essential. The authors discuss the use of medical hypnosis to circumvent general anesthesia and to reduce chemical sedation to a minimum. Their experience with this technique is presented."}
{"id": "PMID:481741", "title": "Persistent trigeminal artery: unusual associated abnormalities.", "content": "Multiple cases of vascular abnormalities associated with the persistent trigeminal artery or a variant thereof are presented. A persistent otic artery variant is the only known such case in the literature.", "contents": "Persistent trigeminal artery: unusual associated abnormalities. Multiple cases of vascular abnormalities associated with the persistent trigeminal artery or a variant thereof are presented. A persistent otic artery variant is the only known such case in the literature."}
{"id": "PMID:481743", "title": "Treatment of external carotid arteriovenous fistula with detachable balloon.", "content": "A simple fistula between the external carotid artery and the internal jugular vein was treated for the first time using Debrun's technique with a detachable balloon catheter. The nature of the fistulous connection makes the release of the balloon inflated with silicone completely harmless. The occlusion of the fistula, while maintaining the patency of the remaining branches of the external carotid artery, proves this technique to be the most appropriate for the treatment of arteriovenous fistulae in the neck.", "contents": "Treatment of external carotid arteriovenous fistula with detachable balloon. A simple fistula between the external carotid artery and the internal jugular vein was treated for the first time using Debrun's technique with a detachable balloon catheter. The nature of the fistulous connection makes the release of the balloon inflated with silicone completely harmless. The occlusion of the fistula, while maintaining the patency of the remaining branches of the external carotid artery, proves this technique to be the most appropriate for the treatment of arteriovenous fistulae in the neck."}
{"id": "PMID:481744", "title": "Occipital approach for treatment of arteriovenous malformation of the vertebral artery by balloon occlusion.", "content": "The successful treatment of a congenital vertebral fistula with the balloon catheter occlusion technique is reported. A new approach is presented (the occipital approach), based upon the pathological enlargement of the second intervertebral space anastomosis between occipital and vertebral arteries.", "contents": "Occipital approach for treatment of arteriovenous malformation of the vertebral artery by balloon occlusion. The successful treatment of a congenital vertebral fistula with the balloon catheter occlusion technique is reported. A new approach is presented (the occipital approach), based upon the pathological enlargement of the second intervertebral space anastomosis between occipital and vertebral arteries."}
{"id": "PMID:481745", "title": "Bilateral occlusion of the cervical internal carotid arteries: a case with an unusual co-lateral circulation.", "content": "The case of a man, aged 67,who had bilateral occlusion of the cervical internal carotid arteries with an unusual collateral circulation between the occipital artery and the anterior cerebral artery, is described.", "contents": "Bilateral occlusion of the cervical internal carotid arteries: a case with an unusual co-lateral circulation. The case of a man, aged 67,who had bilateral occlusion of the cervical internal carotid arteries with an unusual collateral circulation between the occipital artery and the anterior cerebral artery, is described."}
{"id": "PMID:481746", "title": "The neuroradiological findings in a case of cerebral tuberculoma.", "content": "This paper describes the neuroradioloigical findings in a case of cerebral tuberculoma. Computed tomography demonstrated a mass in contact with the cranial bone with marked contrast enhancement and accompanied by perifocal cerebral edema. External carotid angiography revealed a homogenous stain supplied from the middle meningeal artery. Furthermore, by radioisotope scan it appeared to be a region of increased uptake with a comparatively clear border and was thereby misdiagnosed as meningioma.", "contents": "The neuroradiological findings in a case of cerebral tuberculoma. This paper describes the neuroradioloigical findings in a case of cerebral tuberculoma. Computed tomography demonstrated a mass in contact with the cranial bone with marked contrast enhancement and accompanied by perifocal cerebral edema. External carotid angiography revealed a homogenous stain supplied from the middle meningeal artery. Furthermore, by radioisotope scan it appeared to be a region of increased uptake with a comparatively clear border and was thereby misdiagnosed as meningioma."}
{"id": "PMID:481747", "title": "Spinal leptomeningeal carcinosis visualized by amipaque myelography.", "content": "A case of metastases to the spinal subarachnoidal space from a primary anaplastic pulmonary tumor visualized by Amipaque myelography is reported.", "contents": "Spinal leptomeningeal carcinosis visualized by amipaque myelography. A case of metastases to the spinal subarachnoidal space from a primary anaplastic pulmonary tumor visualized by Amipaque myelography is reported."}
{"id": "PMID:481756", "title": "[Interposition of a jejunal loop, using the Mouchet-Camey method, in gastric cancer surgery].", "content": "A series of 80 patients subjected to total gastrectomy for neoplasia is presented. Interposition of the jejunal loop according to Mouchet-Camey was employed as the reconstruction technique. The advantages of this method are highlighted, with particular reference to the results of a study of the absorption of 125I-oleic acid. The complications associated with the operation and the way in which they can be corrected are described.", "contents": "[Interposition of a jejunal loop, using the Mouchet-Camey method, in gastric cancer surgery]. A series of 80 patients subjected to total gastrectomy for neoplasia is presented. Interposition of the jejunal loop according to Mouchet-Camey was employed as the reconstruction technique. The advantages of this method are highlighted, with particular reference to the results of a study of the absorption of 125I-oleic acid. The complications associated with the operation and the way in which they can be corrected are described."}
{"id": "PMID:481757", "title": "[Intrahepatic lithiasis. Apropos of 36 cases of \"regional\" intraheptic lithiasis].", "content": "Out of 2,700 operations for cholelithiasis and its sequelae (1960-1976), 36 cases (1.3%) of intrahepatic lithiasis (i.l.), namely proximal to the origin of the common hepatic, were observed. The prime objective of treatment namely removal of calculi, was achieved indirectly (hepatocholedochus and/or papilla) in 84% of cases, directly (hilar) in 16%. The second objective, that of ensuring optimal bilio-enteric drainage, was achieved by papillostomy or hepaticojejunostomy depending on the lumen of the bile way (respectively less or more than 2 cm.). Operative mortality was nil, while long-term results have proved poor in 9.6% of cases. The i.l. problematic is dealt with in detail on the basis of this series.", "contents": "[Intrahepatic lithiasis. Apropos of 36 cases of \"regional\" intraheptic lithiasis]. Out of 2,700 operations for cholelithiasis and its sequelae (1960-1976), 36 cases (1.3%) of intrahepatic lithiasis (i.l.), namely proximal to the origin of the common hepatic, were observed. The prime objective of treatment namely removal of calculi, was achieved indirectly (hepatocholedochus and/or papilla) in 84% of cases, directly (hilar) in 16%. The second objective, that of ensuring optimal bilio-enteric drainage, was achieved by papillostomy or hepaticojejunostomy depending on the lumen of the bile way (respectively less or more than 2 cm.). Operative mortality was nil, while long-term results have proved poor in 9.6% of cases. The i.l. problematic is dealt with in detail on the basis of this series."}
{"id": "PMID:481759", "title": "[Treatment of the gastric tuberosity in sliding hiatal hernias].", "content": "A technique for the treatment of gastric tuberosity in hiatal hernias due to slippage is presented. It consists of overturning and attachment of the tuberosity to the anterior wall of the stomach and gastropexy according to Niessen. The excellent results obtained with this very simple and by no means traumatic operation suggest that, given the same results with regard to the disappearance of the hernia that the postoperative disturbances observed in a considerable number of cases treated by means of classic fundoplication.", "contents": "[Treatment of the gastric tuberosity in sliding hiatal hernias]. A technique for the treatment of gastric tuberosity in hiatal hernias due to slippage is presented. It consists of overturning and attachment of the tuberosity to the anterior wall of the stomach and gastropexy according to Niessen. The excellent results obtained with this very simple and by no means traumatic operation suggest that, given the same results with regard to the disappearance of the hernia that the postoperative disturbances observed in a considerable number of cases treated by means of classic fundoplication."}
{"id": "PMID:481761", "title": "[Evaluation of survival in a sample of patients treated with radical mastectomy for mammary carcinoma].", "content": "Survival and its modalities were studied in 40 patients subjected to Halsted's operation for breast cancer between 1960 and 1972. The following parameters were considered: age at time of surgery, onset of tumour before or after menopause, age of commencement of menopause, married or single, number of pregnancies, breast feeding, regularity of menstrual cycle, prior breast disease, time elapsed between first symptoms and mastectomy, side affected, quadrant affected, involvement of ipsilateral lymph nodes, histological type, complementary X-ray treatment, ovariectomy, and medical hormone therapy.", "contents": "[Evaluation of survival in a sample of patients treated with radical mastectomy for mammary carcinoma]. Survival and its modalities were studied in 40 patients subjected to Halsted's operation for breast cancer between 1960 and 1972. The following parameters were considered: age at time of surgery, onset of tumour before or after menopause, age of commencement of menopause, married or single, number of pregnancies, breast feeding, regularity of menstrual cycle, prior breast disease, time elapsed between first symptoms and mastectomy, side affected, quadrant affected, involvement of ipsilateral lymph nodes, histological type, complementary X-ray treatment, ovariectomy, and medical hormone therapy."}
{"id": "PMID:481762", "title": "[Behavior of the \"double erythrocyte sedimentation rate\" in neoplastic pathology].", "content": "Special aspects of a particular ESR reading have been evaluated in a series of 273 patients admitted ver a number of years to the Departments of Surgical Anatomy and Operations Course and, thereafter, Surgical Symptomatology of the University of Milan (Head: Prof. Ugo Ruberti). The inhibitory phase of sedimentation observable in vitro is particularly and typically scanty in patients with malignant tumours. The negative results are also analysed critically, with mention of particular factors (metastasis, arteriopathies, etc.) of use in diagnosis and prognosis. In the light of present-day knowledge it is therefore considered that this \"double\" ESR reading points, with sufficient specificity, to an ongoing tumoral process.", "contents": "[Behavior of the \"double erythrocyte sedimentation rate\" in neoplastic pathology]. Special aspects of a particular ESR reading have been evaluated in a series of 273 patients admitted ver a number of years to the Departments of Surgical Anatomy and Operations Course and, thereafter, Surgical Symptomatology of the University of Milan (Head: Prof. Ugo Ruberti). The inhibitory phase of sedimentation observable in vitro is particularly and typically scanty in patients with malignant tumours. The negative results are also analysed critically, with mention of particular factors (metastasis, arteriopathies, etc.) of use in diagnosis and prognosis. In the light of present-day knowledge it is therefore considered that this \"double\" ESR reading points, with sufficient specificity, to an ongoing tumoral process."}
{"id": "PMID:481767", "title": "[Early operation in acute cholecystitis].", "content": "104 patients with acute cholecystitis were divided into four groups according to the kind of pre-operative treatment received: immediate; early; late; and interval operation. Total duration of hospital stay depended on the duration of pre-operative treatment. It was shortest (24.9 days) in those operated on within the first week, longest (71.1 days) in those operated on during the interval. Evidence of severe gallbladder wall changes (macroscopic and microscopic) was obtained in two thirds of patients, even after weeks of conservative treatment. Six patients (5.7%) died postoperatively, three of them after early operation. Overall mortality was only in part due to severe complications of the gallbladder disease.", "contents": "[Early operation in acute cholecystitis]. 104 patients with acute cholecystitis were divided into four groups according to the kind of pre-operative treatment received: immediate; early; late; and interval operation. Total duration of hospital stay depended on the duration of pre-operative treatment. It was shortest (24.9 days) in those operated on within the first week, longest (71.1 days) in those operated on during the interval. Evidence of severe gallbladder wall changes (macroscopic and microscopic) was obtained in two thirds of patients, even after weeks of conservative treatment. Six patients (5.7%) died postoperatively, three of them after early operation. Overall mortality was only in part due to severe complications of the gallbladder disease."}
{"id": "PMID:481763", "title": "[Treatment of esophageal achalasia].", "content": "4 cases of oesophageal achalasia, 2 primary and 2 recurrences following multiple oesophagomyotomy are reported. The superiority of oesophagomyotomy carried out by the left transthoracic route (according to Ellis) in cases of primary achalasia is stressed. On the basis of the literature and reported experience, it is concluded that this approach is also preferable in recurrences when the previous operation was done by the abdominal route and the recurrence occurred within 6 months. In all other cases, particularly in the presence of an achalasic megaoesophagus, partial oesophagogastric resection with interposition of a jejunal loop (Merendino's operation) is the procedure of choice and the satisfactory results of using it are reported.", "contents": "[Treatment of esophageal achalasia]. 4 cases of oesophageal achalasia, 2 primary and 2 recurrences following multiple oesophagomyotomy are reported. The superiority of oesophagomyotomy carried out by the left transthoracic route (according to Ellis) in cases of primary achalasia is stressed. On the basis of the literature and reported experience, it is concluded that this approach is also preferable in recurrences when the previous operation was done by the abdominal route and the recurrence occurred within 6 months. In all other cases, particularly in the presence of an achalasic megaoesophagus, partial oesophagogastric resection with interposition of a jejunal loop (Merendino's operation) is the procedure of choice and the satisfactory results of using it are reported."}
{"id": "PMID:481769", "title": "[Revascularization of the deep femoral artery in arterial insufficiency of the lower limbs. Clinical aspects and immediate results].", "content": "The main indications for revascularisation of the lower limb using the deep femoral artery are explained. In aorto-iliac disease with obstruction of the superficials, an aortofemoral by-pass is created, and the distal end of the prosthesis is sutured on the point of origin of the profunda in the form of a patch. In cases where the patients's local or general condition precludes the establishment of a femoro-popliteal by-pass in femoro-popliteal disease, endoarteriectomy is performed and a patch is applied from the CFA to the first segment of the profunda. Thirty-six cases in which the method described was applied are illustrated. These are culled from a total of 144 artery reconstructions performed in 1975--77. The results (33 asymptomatic cases 6 months after surgery, equal to 91.7%) are comparable with those obtained in by-passes with patent SFA in patients with aorto-iliac obstruction. In addition, the method often makes amputation unnecessary in patients with obstruction of the subinguinal trunks only, but unable to be treated by the establishment of a femoropopliteal by-pass.", "contents": "[Revascularization of the deep femoral artery in arterial insufficiency of the lower limbs. Clinical aspects and immediate results]. The main indications for revascularisation of the lower limb using the deep femoral artery are explained. In aorto-iliac disease with obstruction of the superficials, an aortofemoral by-pass is created, and the distal end of the prosthesis is sutured on the point of origin of the profunda in the form of a patch. In cases where the patients's local or general condition precludes the establishment of a femoro-popliteal by-pass in femoro-popliteal disease, endoarteriectomy is performed and a patch is applied from the CFA to the first segment of the profunda. Thirty-six cases in which the method described was applied are illustrated. These are culled from a total of 144 artery reconstructions performed in 1975--77. The results (33 asymptomatic cases 6 months after surgery, equal to 91.7%) are comparable with those obtained in by-passes with patent SFA in patients with aorto-iliac obstruction. In addition, the method often makes amputation unnecessary in patients with obstruction of the subinguinal trunks only, but unable to be treated by the establishment of a femoropopliteal by-pass."}
{"id": "PMID:481765", "title": "[Cryotherapy of hemorrhoids. Analysis of 40 cases].", "content": "An appraisal is made of the results of cryotherapy in 40 carefully selected cases of haemorrhoids. It is shown that the method is effective if practised in accordance with the correct technique and where specifically indicated. The incidence of possible complications and recurrences is also discussed. Points in favour of the method include its greater tolerability, the possibility of using it in out-patient management, and the fact that it permits an earlier return to work. Attention must be paid to the narrowness of its indications and the anorectal diseases that may often be present.", "contents": "[Cryotherapy of hemorrhoids. Analysis of 40 cases]. An appraisal is made of the results of cryotherapy in 40 carefully selected cases of haemorrhoids. It is shown that the method is effective if practised in accordance with the correct technique and where specifically indicated. The incidence of possible complications and recurrences is also discussed. Points in favour of the method include its greater tolerability, the possibility of using it in out-patient management, and the fact that it permits an earlier return to work. Attention must be paid to the narrowness of its indications and the anorectal diseases that may often be present."}
{"id": "PMID:481766", "title": "[Short-term and long-term clinical study of the absence of nutritional syndromes, body weight increase, blood serum parameters, and return to work of patients gastrectomized using a special surgical technique. III].", "content": "Short- and long-term clinical results obtained following the employment of a surgical technique used for gastric resection, and described in two previous notes, are presented. Nutritional syndromes were not observed and body weight increased. These findings, coupled with the blood serum data and the fact that patients fully resumed their work, are compared with the less satisfactory results obtained with the techniques of Balfour and Reichel-Polya.", "contents": "[Short-term and long-term clinical study of the absence of nutritional syndromes, body weight increase, blood serum parameters, and return to work of patients gastrectomized using a special surgical technique. III]. Short- and long-term clinical results obtained following the employment of a surgical technique used for gastric resection, and described in two previous notes, are presented. Nutritional syndromes were not observed and body weight increased. These findings, coupled with the blood serum data and the fact that patients fully resumed their work, are compared with the less satisfactory results obtained with the techniques of Balfour and Reichel-Polya."}
{"id": "PMID:481772", "title": "[Congenital anomalies of the gallbladder. A case of retrohepatic gallbladder contained in the coronary ligament].", "content": "A case of ectopic gall-bladder located retrohepatically in the coronary ligament is reported. Stress is laid on the interest and extreme rarity of this event which escaped observation at a first explorative laparotomy. Further confirmation is thus provided, within bile way malformations, of the need for attentive intraoperative exploration, including cholangiography.", "contents": "[Congenital anomalies of the gallbladder. A case of retrohepatic gallbladder contained in the coronary ligament]. A case of ectopic gall-bladder located retrohepatically in the coronary ligament is reported. Stress is laid on the interest and extreme rarity of this event which escaped observation at a first explorative laparotomy. Further confirmation is thus provided, within bile way malformations, of the need for attentive intraoperative exploration, including cholangiography."}
{"id": "PMID:481774", "title": "[Surgery of malignant neoplasms of the esophagus].", "content": "A series of malignant cancers of the oesophagus operated in the period 1968-1978 is reported. The importance of preoperative denutrition is considered and the various nutritional techniques are outlined, with a note on their advantages and defects. Right intrathoracic oesophagogastroplasty carried out in malignant cancers of the middle third of the oesophagus is particularly discussed. This surgical technique is held to be preferable to intestinal transpositions in substitution of the oesophagus, and the results obtained in this series support the opinion.", "contents": "[Surgery of malignant neoplasms of the esophagus]. A series of malignant cancers of the oesophagus operated in the period 1968-1978 is reported. The importance of preoperative denutrition is considered and the various nutritional techniques are outlined, with a note on their advantages and defects. Right intrathoracic oesophagogastroplasty carried out in malignant cancers of the middle third of the oesophagus is particularly discussed. This surgical technique is held to be preferable to intestinal transpositions in substitution of the oesophagus, and the results obtained in this series support the opinion."}
{"id": "PMID:481775", "title": "[Surgical treatment in peripheral acute obliterating arterial diseases].", "content": "Vascular surgery of acute cases, embolism and thromboses encountered since 1973 in the Surgical division of Manerbio (BS) hospital is reviewed and reference made to the surgical treatment and results in 78 cases of direct vascular surgery in 68 acute patients. Following schematic presentation of the type of pathology observed and of the types of operation performed, stress is laid on the effectiveness of Vollmar rings in association with traditional Fogarty catheters in thrombectomy operations. The frequent need to associate by-pass or disobliteration of the acutely occluded segment with thrombectomy in acute thrombosis is also pointed out. The varied series involves most acute vascular pathology, inluding sapheno-femoral anastomosis, and the very low mortality and small number of amputations support surgical indications and the techniques employed.", "contents": "[Surgical treatment in peripheral acute obliterating arterial diseases]. Vascular surgery of acute cases, embolism and thromboses encountered since 1973 in the Surgical division of Manerbio (BS) hospital is reviewed and reference made to the surgical treatment and results in 78 cases of direct vascular surgery in 68 acute patients. Following schematic presentation of the type of pathology observed and of the types of operation performed, stress is laid on the effectiveness of Vollmar rings in association with traditional Fogarty catheters in thrombectomy operations. The frequent need to associate by-pass or disobliteration of the acutely occluded segment with thrombectomy in acute thrombosis is also pointed out. The varied series involves most acute vascular pathology, inluding sapheno-femoral anastomosis, and the very low mortality and small number of amputations support surgical indications and the techniques employed."}
{"id": "PMID:481776", "title": "[Personal clinical and neurophysiological experience in the surgically treated carpal tunnel syndrome].", "content": "In 18 cases of carpal tunnel syndrome treated by surgical decompression of the median nerve, a neurophysiological control was performed before and after surgery. In all cases improvement or disappearance of the pain was observed; improvement in motor function was less constant. Control of the median nerve conduction showed that in all cases with preoperative distal latency less than 6 msec. there was a normalization of the nerve conduction after only 3 months. In cases of distal latency over 6 msec, and especially in those with complete motor conduction block, the latency remain within patological limits even 15 months after surgery. This may be explained either by a slower conduction of the regenerated fibres or by a persistent myelinic and axonal damage of the nerve.", "contents": "[Personal clinical and neurophysiological experience in the surgically treated carpal tunnel syndrome]. In 18 cases of carpal tunnel syndrome treated by surgical decompression of the median nerve, a neurophysiological control was performed before and after surgery. In all cases improvement or disappearance of the pain was observed; improvement in motor function was less constant. Control of the median nerve conduction showed that in all cases with preoperative distal latency less than 6 msec. there was a normalization of the nerve conduction after only 3 months. In cases of distal latency over 6 msec, and especially in those with complete motor conduction block, the latency remain within patological limits even 15 months after surgery. This may be explained either by a slower conduction of the regenerated fibres or by a persistent myelinic and axonal damage of the nerve."}
{"id": "PMID:481779", "title": "[Possibility of applying dynamic electrocardiography to function studies in flight].", "content": "A new dynamic ECG apparatus that has been operating since last June in a Roman Diagnosis Centre is described. The apparatus enables pilot cardiovascular function to be studied during flight. On the basis of long personal experience of tourist flying (single and twin engine craft), the exceptional usefulness of this apparatus for monitoring changes which the stress of flying can provoke in a pilot's cardiovascular apparatus is pointed out.", "contents": "[Possibility of applying dynamic electrocardiography to function studies in flight]. A new dynamic ECG apparatus that has been operating since last June in a Roman Diagnosis Centre is described. The apparatus enables pilot cardiovascular function to be studied during flight. On the basis of long personal experience of tourist flying (single and twin engine craft), the exceptional usefulness of this apparatus for monitoring changes which the stress of flying can provoke in a pilot's cardiovascular apparatus is pointed out."}
{"id": "PMID:481782", "title": "[International anti-malaria prevention today for the Italian airline].", "content": "The ease with which malaria can be transmitted over long distances as a result of air transport is illustrated with reference to the documentary evidence. The best ways of preventing this risk are also described.", "contents": "[International anti-malaria prevention today for the Italian airline]. The ease with which malaria can be transmitted over long distances as a result of air transport is illustrated with reference to the documentary evidence. The best ways of preventing this risk are also described."}
{"id": "PMID:481784", "title": "[New directions in radiotherapy. The use of fast electrons].", "content": "The post-surgical radiotherapy of breast cancer and its problems are discussed and an account is given of recent trends in this field. The use of fast electrons is regarded as an optimal form of management for the chest wall, and the ipsilateral subscapular and internal mammary lymph gland chains.", "contents": "[New directions in radiotherapy. The use of fast electrons]. The post-surgical radiotherapy of breast cancer and its problems are discussed and an account is given of recent trends in this field. The use of fast electrons is regarded as an optimal form of management for the chest wall, and the ipsilateral subscapular and internal mammary lymph gland chains."}
{"id": "PMID:481785", "title": "[Erythrocyte protective synergism of ascorbic acid and alpha-mercaptopropionylglycine].", "content": "The antitoxic action of 1-ascorbic acid and alpha-mercaptopropionyl glycine was examined via the formation of Ehrlich-Heinz bodies in human venous red cells following in vitro intoxication with 100 mg/ml acetylphenylhydrazine. Marked synergism was noted, especially when small amounts of glucose were added. Both substances displayed a marked affinity for red cells and penetrated them on contact without an incubation period. Optimum amounts for maximum synergy were alpha-mercaptopropionyl glycine 0.01-0.02, 1-ascorbic acid 0.01-0.02 ml and glucose 40 mg per ml of blood. At this dose, appearance of Ehrlich bodies was appreciably retarded. In addition, changes in the size and quality of such bodies pointed to enhanced cell resistance to the poison used.", "contents": "[Erythrocyte protective synergism of ascorbic acid and alpha-mercaptopropionylglycine]. The antitoxic action of 1-ascorbic acid and alpha-mercaptopropionyl glycine was examined via the formation of Ehrlich-Heinz bodies in human venous red cells following in vitro intoxication with 100 mg/ml acetylphenylhydrazine. Marked synergism was noted, especially when small amounts of glucose were added. Both substances displayed a marked affinity for red cells and penetrated them on contact without an incubation period. Optimum amounts for maximum synergy were alpha-mercaptopropionyl glycine 0.01-0.02, 1-ascorbic acid 0.01-0.02 ml and glucose 40 mg per ml of blood. At this dose, appearance of Ehrlich bodies was appreciably retarded. In addition, changes in the size and quality of such bodies pointed to enhanced cell resistance to the poison used."}
{"id": "PMID:481787", "title": "[Circadian variations in glycemia and IRI in normal subjects, young and adult diabetics and in obese subjects].", "content": "Blood sugar and IRI were followed for 24 hr in healthy patients, young and adult diabetics, and in subjects with apparently primary obesity on a standard, normal calorie diet. Morning and nocturnal insulin positivity peaks were noted in the normal subjects, though circadians variations in these parameters were mainly related to meals. This periodicity was altered in young diabetics, for whom insulin responsivity was ruled out, and in the adult diabetics, who displayed deficient insulin secretion in the morning, and delayed secretion after the afternoon meal, and in the obese subjects, the quality of whose response was close to that of the previous group. The usefulness of this type on examination in the planning of diet and drug management, quite apart from diagnosis and prognosis, is stressed.", "contents": "[Circadian variations in glycemia and IRI in normal subjects, young and adult diabetics and in obese subjects]. Blood sugar and IRI were followed for 24 hr in healthy patients, young and adult diabetics, and in subjects with apparently primary obesity on a standard, normal calorie diet. Morning and nocturnal insulin positivity peaks were noted in the normal subjects, though circadians variations in these parameters were mainly related to meals. This periodicity was altered in young diabetics, for whom insulin responsivity was ruled out, and in the adult diabetics, who displayed deficient insulin secretion in the morning, and delayed secretion after the afternoon meal, and in the obese subjects, the quality of whose response was close to that of the previous group. The usefulness of this type on examination in the planning of diet and drug management, quite apart from diagnosis and prognosis, is stressed."}
{"id": "PMID:481788", "title": "[Pseudo-tumoral calcinosis universalis].", "content": "Marked deposits of calcium salts were noted in the triceps and gluteal and peritrochanteric areas in a 16-yr-old girl. Blood calcium values basal and after stimulation were normal. The classification of the case as a form of pseudotumoral calcinosis universalis is discussed.", "contents": "[Pseudo-tumoral calcinosis universalis]. Marked deposits of calcium salts were noted in the triceps and gluteal and peritrochanteric areas in a 16-yr-old girl. Blood calcium values basal and after stimulation were normal. The classification of the case as a form of pseudotumoral calcinosis universalis is discussed."}
{"id": "PMID:481789", "title": "[Sporadic persistence of amebic hepatitis].", "content": "A case of classical colliquative amoebic hepatitis, documented with aspiration of abundant amoebic pus by means of hepatocentesis and treated with chloroquine and haematin, is reported. This type of amoebiasis is rare nowadays, it is pointed out. Present-day clinico-epidemiological features are outlined: reduction in official cases, reversal of the ratio between colitis and hepatitis (the latter is on the increase), disappearance of acute dysenteric forms, less tendency to colliquative development in cases where the liver is involved, an involvement which is seen most frequently with an atypical chronic-type imprint. Mention is finally made of the reasons behind this cliniqo-epidemiological shift.", "contents": "[Sporadic persistence of amebic hepatitis]. A case of classical colliquative amoebic hepatitis, documented with aspiration of abundant amoebic pus by means of hepatocentesis and treated with chloroquine and haematin, is reported. This type of amoebiasis is rare nowadays, it is pointed out. Present-day clinico-epidemiological features are outlined: reduction in official cases, reversal of the ratio between colitis and hepatitis (the latter is on the increase), disappearance of acute dysenteric forms, less tendency to colliquative development in cases where the liver is involved, an involvement which is seen most frequently with an atypical chronic-type imprint. Mention is finally made of the reasons behind this cliniqo-epidemiological shift."}
{"id": "PMID:481790", "title": "[A case of pulmonary eosinophilia with protracted and benign course].", "content": "A case of corticosteroid-sensitive pulmonary eosinophilia with protracted course, followed up for more than years, is reported. After highlighting the diagnostic difficulties of such clinical pictures and their as yet ill-defined nosological contours, the comparative benignity of most of such forms is stressed.", "contents": "[A case of pulmonary eosinophilia with protracted and benign course]. A case of corticosteroid-sensitive pulmonary eosinophilia with protracted course, followed up for more than years, is reported. After highlighting the diagnostic difficulties of such clinical pictures and their as yet ill-defined nosological contours, the comparative benignity of most of such forms is stressed."}
{"id": "PMID:481792", "title": "[Basic aspects and current trends in iatrogenic kidney diseases. Correlations between their physiopathological mechanisms and clinical findings for the general practitioner].", "content": "Iatrogenic origins may be assigned to any form of kidney disease. Of the pathogenetic mechanisms involved, increasing importance is now attached to immunological responses and intravascular coagulopathies, though other processes long known to medicine have not ceased to play their part. Moreover, recent advances in the improvement of drugs and the standardisation of their use have given a better idea of the potential damage to the kidneys from many substances. A re-examination is made of the main findings in this field with a view to offering a simple summary of the underlying physiopathogenetic mechanisms, and drawing the diagnostic and clinical conclusions therefrom, so as to aid the general practitioner rather than the specialist. An account of the various medical interventions that may lead to kidney lesions, and the circumstances in which such interventions are most likely to be harmful, is followed by an analysis of recent opinions on the correct way of administering drugs in patients with kidney disease, so as to limit their potentially harmful effects as far as possible.", "contents": "[Basic aspects and current trends in iatrogenic kidney diseases. Correlations between their physiopathological mechanisms and clinical findings for the general practitioner]. Iatrogenic origins may be assigned to any form of kidney disease. Of the pathogenetic mechanisms involved, increasing importance is now attached to immunological responses and intravascular coagulopathies, though other processes long known to medicine have not ceased to play their part. Moreover, recent advances in the improvement of drugs and the standardisation of their use have given a better idea of the potential damage to the kidneys from many substances. A re-examination is made of the main findings in this field with a view to offering a simple summary of the underlying physiopathogenetic mechanisms, and drawing the diagnostic and clinical conclusions therefrom, so as to aid the general practitioner rather than the specialist. An account of the various medical interventions that may lead to kidney lesions, and the circumstances in which such interventions are most likely to be harmful, is followed by an analysis of recent opinions on the correct way of administering drugs in patients with kidney disease, so as to limit their potentially harmful effects as far as possible."}
{"id": "PMID:481795", "title": "[Indirect immunoelectrophoresis in the study of urinary proteins].", "content": "The indirect immunoelectrophoresis applied to the proteinurias is a simple and quick method, of usual execution, that makes use of not concentrated urines and discriminant between physiological and pathological proteinurias. The results obtained from a various casistic, collected in many years are presented. For the correlation which exist between morphological isototype and immunoelectropherogram, sometimes, it is possible to go up to the diagnosis of nephropathy also without applying to the renal biopsy.", "contents": "[Indirect immunoelectrophoresis in the study of urinary proteins]. The indirect immunoelectrophoresis applied to the proteinurias is a simple and quick method, of usual execution, that makes use of not concentrated urines and discriminant between physiological and pathological proteinurias. The results obtained from a various casistic, collected in many years are presented. For the correlation which exist between morphological isototype and immunoelectropherogram, sometimes, it is possible to go up to the diagnosis of nephropathy also without applying to the renal biopsy."}
{"id": "PMID:481796", "title": "[The value of electrophoretic studies of urinary proteins in clinical practice].", "content": "A brief account of the physiopathology of proteinuria is followed by a description of the diagnostic routine to be followed in such cases, with particular reference to the electrophoretic study of urinary proteins. In many cases, this simple method gives information on a par with that offered by biopsy, especially insofar as the planning of treatment is concerned. Several clinical cases are cited by way of illustration.", "contents": "[The value of electrophoretic studies of urinary proteins in clinical practice]. A brief account of the physiopathology of proteinuria is followed by a description of the diagnostic routine to be followed in such cases, with particular reference to the electrophoretic study of urinary proteins. In many cases, this simple method gives information on a par with that offered by biopsy, especially insofar as the planning of treatment is concerned. Several clinical cases are cited by way of illustration."}
{"id": "PMID:481797", "title": "[Incidence of skin changes during active chronic hepatitis and liver cirrhosis. Comparison with normal controls].", "content": "The frequency of the principal skin changes held to be typical of chronic diseases of the liver has been evaluated together with the frequency of diseases of primarily dermatological interest in a group of 116 chronic liver patients (73 with cirrhosis, 43 with active chronic hepatitis) and in a group of 90 controls comparable by sex and age. The frequency of skin changes secondary to liver disease was statistically higher in the patients with respect to the control group. The incidence of primarily skin diseases associated with liver disease was, by contrast, extremely rare. The simultaneous presence of spiders, hepatic palms and white nails was observed in 21% of the chronic liver patients, while it was never encountered in the controls. Clinical observation of these changes (particularly the triad mentioned) is therefore highly indicative of chronic liver disease, even without a significant clinical history.", "contents": "[Incidence of skin changes during active chronic hepatitis and liver cirrhosis. Comparison with normal controls]. The frequency of the principal skin changes held to be typical of chronic diseases of the liver has been evaluated together with the frequency of diseases of primarily dermatological interest in a group of 116 chronic liver patients (73 with cirrhosis, 43 with active chronic hepatitis) and in a group of 90 controls comparable by sex and age. The frequency of skin changes secondary to liver disease was statistically higher in the patients with respect to the control group. The incidence of primarily skin diseases associated with liver disease was, by contrast, extremely rare. The simultaneous presence of spiders, hepatic palms and white nails was observed in 21% of the chronic liver patients, while it was never encountered in the controls. Clinical observation of these changes (particularly the triad mentioned) is therefore highly indicative of chronic liver disease, even without a significant clinical history."}
{"id": "PMID:481798", "title": "[Senile liver and its repercussions on cerebral activity].", "content": "On the basis of previous personal data regarding glycidic, lipidic and protein metabolism in groups of young-old-cirrhotic and hypoglycaemic subjects, a correlative study has been carried out for the purpose of highlighting dependence situations between reduced cerebral activity in the elderly and liver-dependent metabolic changes. Specifically as regards protein metabolism, it is shown that all amino acids dependent on the senescence process (glutammic acid, cystine, thyrosine, histidine, threonine, serine) are related to those involved in altered consciousness states (hepatic and hypoglycaemic coma). It is thus possible that in the elderly too there may be some correlation between liver function deficiency and senile encephalopathy.", "contents": "[Senile liver and its repercussions on cerebral activity]. On the basis of previous personal data regarding glycidic, lipidic and protein metabolism in groups of young-old-cirrhotic and hypoglycaemic subjects, a correlative study has been carried out for the purpose of highlighting dependence situations between reduced cerebral activity in the elderly and liver-dependent metabolic changes. Specifically as regards protein metabolism, it is shown that all amino acids dependent on the senescence process (glutammic acid, cystine, thyrosine, histidine, threonine, serine) are related to those involved in altered consciousness states (hepatic and hypoglycaemic coma). It is thus possible that in the elderly too there may be some correlation between liver function deficiency and senile encephalopathy."}
{"id": "PMID:481799", "title": "[Angina syndromes. Clinical data and case contributions from an emergency ward].", "content": "The Authours wish to do a personal contribution to the anginosis syndromes. We have a series of fifty-two cases divided in various syndromes. We have, then, examined the possible factors of coronary risk, such as arterious hypertension, smoke, diabetes, hypercholesterolaemia.", "contents": "[Angina syndromes. Clinical data and case contributions from an emergency ward]. The Authours wish to do a personal contribution to the anginosis syndromes. We have a series of fifty-two cases divided in various syndromes. We have, then, examined the possible factors of coronary risk, such as arterious hypertension, smoke, diabetes, hypercholesterolaemia."}
{"id": "PMID:481800", "title": "[The chordoma. Description of 2 clinical cases and results of oncolytic chemotherapy].", "content": "Only seven hundred cases of chordoma approximatively are reported in medical literature from 1850 to date. We now describe two cases recently observed at our Medical Division, one of sacro-coccigeal chordoma and the other developed from clivus of Blumenbach. Their peculiarities are briefly discussed and compared with a rapid revision of the literature on this argument. The results of antiblastic therapy are presented and its opportunity in addition to Roentgenologic and surgical therapy is discussed.", "contents": "[The chordoma. Description of 2 clinical cases and results of oncolytic chemotherapy]. Only seven hundred cases of chordoma approximatively are reported in medical literature from 1850 to date. We now describe two cases recently observed at our Medical Division, one of sacro-coccigeal chordoma and the other developed from clivus of Blumenbach. Their peculiarities are briefly discussed and compared with a rapid revision of the literature on this argument. The results of antiblastic therapy are presented and its opportunity in addition to Roentgenologic and surgical therapy is discussed."}
{"id": "PMID:481801", "title": "[Pelger-Huet leukocyte anomaly. A clinical case of acquired and transient anomaly].", "content": "A survey of the relevant literature is followed by the presentation of a case of transient, acquired Pelger-Hu\u00ebt anomaly observed in a patient operated for Crohn's disease.", "contents": "[Pelger-Huet leukocyte anomaly. A clinical case of acquired and transient anomaly]. A survey of the relevant literature is followed by the presentation of a case of transient, acquired Pelger-Hu\u00ebt anomaly observed in a patient operated for Crohn's disease."}
{"id": "PMID:481821", "title": "[An elderly patient with symptoms of dehydration].", "content": "In connection with serious dehydration symptoms in an elderly patient, which appeared to be attributable to a hypersmolar nonketoacidotic diabetic syndrome, a comprehensive study of this syndrome is made with reference to a study of pertinent literature. Possible causes, pathogenesis, clinic and treatment are extensively dealt with. The seriousness of this clinical picture, the high rate of mortality and the favourable prognosis if immediate action is taken are pointed out.", "contents": "[An elderly patient with symptoms of dehydration]. In connection with serious dehydration symptoms in an elderly patient, which appeared to be attributable to a hypersmolar nonketoacidotic diabetic syndrome, a comprehensive study of this syndrome is made with reference to a study of pertinent literature. Possible causes, pathogenesis, clinic and treatment are extensively dealt with. The seriousness of this clinical picture, the high rate of mortality and the favourable prognosis if immediate action is taken are pointed out."}
{"id": "PMID:481822", "title": "[We all will age].", "content": "In 1977/78 the American Research-plan toward understanding aging 'Our future selves' was published. In this article the report of the panel on biomedical research is reviewed. The report appears to be very comprehensive and to give a carefully detailed research plan. It will certainly take many years to realise all the plans. Part of the research could very well be done in the Netherlands although only to a limited extent. The governmental planning-group, which is developing a research plan for gerontology in the Netherlands, should make a sensible selection out of the many items mentioned in the American plan. After all, it is of vital importance to all of us, our future selves.", "contents": "[We all will age]. In 1977/78 the American Research-plan toward understanding aging 'Our future selves' was published. In this article the report of the panel on biomedical research is reviewed. The report appears to be very comprehensive and to give a carefully detailed research plan. It will certainly take many years to realise all the plans. Part of the research could very well be done in the Netherlands although only to a limited extent. The governmental planning-group, which is developing a research plan for gerontology in the Netherlands, should make a sensible selection out of the many items mentioned in the American plan. After all, it is of vital importance to all of us, our future selves."}
{"id": "PMID:481823", "title": "[Our future selves: individual behavioral aspects. Discussion and comments].", "content": "We will summarize this part of the plan against a historical background (Havighurst, 1969, 1071; the second White House Conference on Aging, 1971) and compare some parts (personality and adaptation, death and dying) with the list of priorities in the 'summary report'. Policy- making and planning research appear to be too different tasks. Our comments concern especially: - the choice of the design for the research-plan; - too little is said in the plan on the conditions (money, research centers, education, necessary for realization); - themes missing: the meaning of old age, given by the individual older person in connection to time-experience; sexuality and aging; suicide and pre-aging mortality; - the splitting of the research-plan in three parts has advantages but also disadvantages. There are three (independent) fields with not enough overlap, i.e. not enough interdisciplinary research-proposals; - another point is the unequal level of testability of the different proposed themes; - the last point is, that the concept of aging, which forms the background, [corrected] is a rather negative one. Therefore, few proposals concern the positive aspects of growing older and being old. This is perhaps related to the handicap, that there is not one general theory on aging (if that is possible?).", "contents": "[Our future selves: individual behavioral aspects. Discussion and comments]. We will summarize this part of the plan against a historical background (Havighurst, 1969, 1071; the second White House Conference on Aging, 1971) and compare some parts (personality and adaptation, death and dying) with the list of priorities in the 'summary report'. Policy- making and planning research appear to be too different tasks. Our comments concern especially: - the choice of the design for the research-plan; - too little is said in the plan on the conditions (money, research centers, education, necessary for realization); - themes missing: the meaning of old age, given by the individual older person in connection to time-experience; sexuality and aging; suicide and pre-aging mortality; - the splitting of the research-plan in three parts has advantages but also disadvantages. There are three (independent) fields with not enough overlap, i.e. not enough interdisciplinary research-proposals; - another point is the unequal level of testability of the different proposed themes; - the last point is, that the concept of aging, which forms the background, [corrected] is a rather negative one. Therefore, few proposals concern the positive aspects of growing older and being old. This is perhaps related to the handicap, that there is not one general theory on aging (if that is possible?)."}
{"id": "PMID:481824", "title": "[What is at stake? 'Our future selves'!].", "content": "The sociological questions in the gerontological research-plan 'Our future selves', developed in the USA for stimulating and co-ordinating the research in this field, are shortly summarized. It concerns demographic studies, age as dimension of the social structure, social-cultural comparative studies, social roles and social networks and the aging problems in different sectors of the society. After this summary some critical remarks are made and some points are mentioned that seem more or less fundamental in this field, namely the societal orientation on youth, the long term orientation that is needed because of the changing demographic structure, the problem of the 'social construction' of aging and the interdependency between the development of the sciences and characteristics of modern society (especially for the situation of old people).", "contents": "[What is at stake? 'Our future selves'!]. The sociological questions in the gerontological research-plan 'Our future selves', developed in the USA for stimulating and co-ordinating the research in this field, are shortly summarized. It concerns demographic studies, age as dimension of the social structure, social-cultural comparative studies, social roles and social networks and the aging problems in different sectors of the society. After this summary some critical remarks are made and some points are mentioned that seem more or less fundamental in this field, namely the societal orientation on youth, the long term orientation that is needed because of the changing demographic structure, the problem of the 'social construction' of aging and the interdependency between the development of the sciences and characteristics of modern society (especially for the situation of old people)."}
{"id": "PMID:481825", "title": "[Services for the aged in the new world. Comments on 'Our future selves: research-plan towards understanding aging'].", "content": "A comment is given on the report 'Our future selves; a research-plan towards understanding aging', concerning the human services and delivery systems. The title of the report seems to promise a picture of the aged in the future. It is regretted that the contents of the report only gives some ideas to improve the current services for the elderly. About the procedure in which the report was achieved is said, that the use of a panel of experts requires a very careful choice of the experts. Next to that, you cannot use the saying of a panel as they are, but you have to work them up, if you want to make a research-lan. As it seems that this is not sufficiently done, the results is not a plan, but a mere collection of subjects. Out of this collection five subjects are described (cultural factors, religion-ethics, nutrition, legal services, commerce), on which in the Netherlands little or no research is done. Participation in geronotology by anthropologists, theologians, and jurists is pleaded.", "contents": "[Services for the aged in the new world. Comments on 'Our future selves: research-plan towards understanding aging']. A comment is given on the report 'Our future selves; a research-plan towards understanding aging', concerning the human services and delivery systems. The title of the report seems to promise a picture of the aged in the future. It is regretted that the contents of the report only gives some ideas to improve the current services for the elderly. About the procedure in which the report was achieved is said, that the use of a panel of experts requires a very careful choice of the experts. Next to that, you cannot use the saying of a panel as they are, but you have to work them up, if you want to make a research-lan. As it seems that this is not sufficiently done, the results is not a plan, but a mere collection of subjects. Out of this collection five subjects are described (cultural factors, religion-ethics, nutrition, legal services, commerce), on which in the Netherlands little or no research is done. Participation in geronotology by anthropologists, theologians, and jurists is pleaded."}
{"id": "PMID:481826", "title": "['Our future selves'. Comments on \"Human services and delivery systems'].", "content": "A description is given of the recommended research topics on Human Services and Delivery Systems for the Aging. Fifteen different fields of research attention are described. It is stated that there is a lack of a frame of reference for the different attention fields. This frame of reference is necessary for a more systematic approach towards the age specific problems and the age specific services, needed to solve those problems. The systematic approach can be found in a three-dimensional scheme in which the fifteen attention fields have their logical place. The recommanded research topics have to be assessed in this frame: 1. Socio-cultural aspects. Culture, religion, ethics, communication, education, recreation, civic participation. 2. Socio-economic aspects. Economic support, employment, legal services, commerce. 3. Physical environment. Physical Environment, nutrition, transportation. 4. Human environment. This aspect is not mentioned in the report. 5. Psychological aspects. 6. Physical aspects. These aspects are not explicitly mentioned in the report, but implicitly in the chapter about Health. Some of the recommendations are also relevant for the situation in the Netherlands. The recommended research on the health care system is critisized, because the items are too complex. For the Netherlands it is more worthwhile to do fundamental research on the age-specific vulnerability in relation to social, psychological and physical aspects. The results of that research can be used for a reconstruction of the whole health care system for the aged.", "contents": "['Our future selves'. Comments on \"Human services and delivery systems']. A description is given of the recommended research topics on Human Services and Delivery Systems for the Aging. Fifteen different fields of research attention are described. It is stated that there is a lack of a frame of reference for the different attention fields. This frame of reference is necessary for a more systematic approach towards the age specific problems and the age specific services, needed to solve those problems. The systematic approach can be found in a three-dimensional scheme in which the fifteen attention fields have their logical place. The recommanded research topics have to be assessed in this frame: 1. Socio-cultural aspects. Culture, religion, ethics, communication, education, recreation, civic participation. 2. Socio-economic aspects. Economic support, employment, legal services, commerce. 3. Physical environment. Physical Environment, nutrition, transportation. 4. Human environment. This aspect is not mentioned in the report. 5. Psychological aspects. 6. Physical aspects. These aspects are not explicitly mentioned in the report, but implicitly in the chapter about Health. Some of the recommendations are also relevant for the situation in the Netherlands. The recommended research on the health care system is critisized, because the items are too complex. For the Netherlands it is more worthwhile to do fundamental research on the age-specific vulnerability in relation to social, psychological and physical aspects. The results of that research can be used for a reconstruction of the whole health care system for the aged."}
{"id": "PMID:481827", "title": "[Vitamin and mineral needs of the aged].", "content": "With increasing age the need of vitamins and minerals is very probably not less than at a younger age. On the other hand, based on a lowered caloric intake and/or a one-sided diet, the possibility of the occurrence of deficiencies will be greater. Resorption disturbances, less body activity, misuse of laxantia and other factors may also be of a negative effect. Quite a number of publications point to the possibility of latent deficiencies, as shown by lowered vitamin and mineral blood levels. Although the available data are scare, older people are certainly a very vulnerable group, in need of special care with regard to their diet, quantitatively as well as qualitatively.", "contents": "[Vitamin and mineral needs of the aged]. With increasing age the need of vitamins and minerals is very probably not less than at a younger age. On the other hand, based on a lowered caloric intake and/or a one-sided diet, the possibility of the occurrence of deficiencies will be greater. Resorption disturbances, less body activity, misuse of laxantia and other factors may also be of a negative effect. Quite a number of publications point to the possibility of latent deficiencies, as shown by lowered vitamin and mineral blood levels. Although the available data are scare, older people are certainly a very vulnerable group, in need of special care with regard to their diet, quantitatively as well as qualitatively."}
{"id": "PMID:481828", "title": "[Fluid deficiency in the older person].", "content": "With reference to the frequent occurrence of dehydration in elderly patients, after a discussion of the causes, the various methods of research are pointed out to determine the lack of moisture, the method of treatment and the prophylaxis of this disorder in the urinary organs.", "contents": "[Fluid deficiency in the older person]. With reference to the frequent occurrence of dehydration in elderly patients, after a discussion of the causes, the various methods of research are pointed out to determine the lack of moisture, the method of treatment and the prophylaxis of this disorder in the urinary organs."}
{"id": "PMID:481829", "title": "Effect of different dietary fats on daily loss of sterols from the skin of man.", "content": "We studied the effect of polyunsaturated dietary fat upon the 24-hour loss of cholesterol and other sterols from the skin surface of 4 human subjects. They were fed cholesterol-free diets which provided 40% of the total caloric intake from saturated fat (cocoa butter) and then a highly polyunsaturated fat (corn oil). In both dietary fat periods, the daily loss of cholesterol through the skin was similar, 91 and 87 mg in the saturated and polyunsaturated fat periods, respectively. 87 and 89% of the total cholesterol were in the esterified form in the saturated and polyunsaturated dietary fat periods. The sterol composition of the skin surface lipid was not altered. The study suggests that the plasma cholesterol-lowering effect of dietary polyunsaturated fat was not mediated by a change in the loss of cholesterol through human skin.", "contents": "Effect of different dietary fats on daily loss of sterols from the skin of man. We studied the effect of polyunsaturated dietary fat upon the 24-hour loss of cholesterol and other sterols from the skin surface of 4 human subjects. They were fed cholesterol-free diets which provided 40% of the total caloric intake from saturated fat (cocoa butter) and then a highly polyunsaturated fat (corn oil). In both dietary fat periods, the daily loss of cholesterol through the skin was similar, 91 and 87 mg in the saturated and polyunsaturated fat periods, respectively. 87 and 89% of the total cholesterol were in the esterified form in the saturated and polyunsaturated dietary fat periods. The sterol composition of the skin surface lipid was not altered. The study suggests that the plasma cholesterol-lowering effect of dietary polyunsaturated fat was not mediated by a change in the loss of cholesterol through human skin."}
{"id": "PMID:481830", "title": "Simultaneous correction of Ca deficiency and acidosis in fasting obese patients as a prevention of bone demineralisation.", "content": "Short-term starvation in the obese induces metabolic acidosis and increased calciuria resulting in severe negative Ca balance. Oral administration of 5 g Ca-lactate daily is capable of correcting acidosis, reducing calciuria and providing the body with 650 mg Ca daily, thereby maintaining a positive Ca balance throughout the period of starvation. We therefore propose administering this amount of the preparation to obese individuals during starvation as a simple and cheap preventive measure against bone demineralisation.", "contents": "Simultaneous correction of Ca deficiency and acidosis in fasting obese patients as a prevention of bone demineralisation. Short-term starvation in the obese induces metabolic acidosis and increased calciuria resulting in severe negative Ca balance. Oral administration of 5 g Ca-lactate daily is capable of correcting acidosis, reducing calciuria and providing the body with 650 mg Ca daily, thereby maintaining a positive Ca balance throughout the period of starvation. We therefore propose administering this amount of the preparation to obese individuals during starvation as a simple and cheap preventive measure against bone demineralisation."}
{"id": "PMID:481831", "title": "Effect of indigestible polysaccharides on protein digestibility and nitrogen retention in growing rats.", "content": "The effect of five indigestible polysaccharides (IP) of different origin and chemical structure on N balance and dry matter digestibility was studied in growing rats. The diets contained 10% of gum guar (GG), carob bean gum (CBG), Na-alginate (Na-A), agar-agar (AA) or carrageenan (C), respectively. Dry matter digestibility and apparent protein digestibility were significantly decreased below control values by all 5 IP. N retention was significantly lower after ingestion of AA or C. In the rats receiving GG, CBG or Na-A urinary N excretion was reduced, thus compensating for increased fecal N losses by these animals. The different effects of the 5 substances on N balance are attributed to their partial breakdown through the action of intestinal microflora and/or their ability to partially inhibit the activity of proteolytic enzymes in the gastrointestinal tract. Trypsin inhibition in vitro was observed for C only. Suitability of the various IP as bulking agents in reducing diets is discussed.", "contents": "Effect of indigestible polysaccharides on protein digestibility and nitrogen retention in growing rats. The effect of five indigestible polysaccharides (IP) of different origin and chemical structure on N balance and dry matter digestibility was studied in growing rats. The diets contained 10% of gum guar (GG), carob bean gum (CBG), Na-alginate (Na-A), agar-agar (AA) or carrageenan (C), respectively. Dry matter digestibility and apparent protein digestibility were significantly decreased below control values by all 5 IP. N retention was significantly lower after ingestion of AA or C. In the rats receiving GG, CBG or Na-A urinary N excretion was reduced, thus compensating for increased fecal N losses by these animals. The different effects of the 5 substances on N balance are attributed to their partial breakdown through the action of intestinal microflora and/or their ability to partially inhibit the activity of proteolytic enzymes in the gastrointestinal tract. Trypsin inhibition in vitro was observed for C only. Suitability of the various IP as bulking agents in reducing diets is discussed."}
{"id": "PMID:481832", "title": "Adenine nucleotide translocation in liver mitochondria isolated from rats deficient in essential fatty acids.", "content": "Adenine nucleotide content and adenine nucleotide transport were evaluated in rats deficient in essential fatty acids (EFA) and in control rats. ADP uptake by EFA-deficient mitochondria was altered in a manner similar to the alteration produced by treatment of normal mitochondria with uncoupler. The uptake of ATP by EFA-deficient mitochondria was more rapid than that of normal mitochondria, but similar to that of normal mitochondria treated with uncoupler (DNP). Both uptake of ADP and uptake of ATP by EFA-deficient mitochondria were atractyloside sensitive. Total adenine nucleotide content of liver mitochondria from EFA-deficient rats was similar to that of liver mitochondria from control animals, but the content of ATP in EFA-deficient mitochondria was significantly higher than that of normal mitochondria. There was a negative correlation between the concentration of linoleic acid in total mitochondria lipids and ATP content of mitochondria.", "contents": "Adenine nucleotide translocation in liver mitochondria isolated from rats deficient in essential fatty acids. Adenine nucleotide content and adenine nucleotide transport were evaluated in rats deficient in essential fatty acids (EFA) and in control rats. ADP uptake by EFA-deficient mitochondria was altered in a manner similar to the alteration produced by treatment of normal mitochondria with uncoupler. The uptake of ATP by EFA-deficient mitochondria was more rapid than that of normal mitochondria, but similar to that of normal mitochondria treated with uncoupler (DNP). Both uptake of ADP and uptake of ATP by EFA-deficient mitochondria were atractyloside sensitive. Total adenine nucleotide content of liver mitochondria from EFA-deficient rats was similar to that of liver mitochondria from control animals, but the content of ATP in EFA-deficient mitochondria was significantly higher than that of normal mitochondria. There was a negative correlation between the concentration of linoleic acid in total mitochondria lipids and ATP content of mitochondria."}
{"id": "PMID:481842", "title": "Selected sulfhydryl inhibitors capable of inducing immunity against cancer in mice.", "content": "Selected sulfhydryl inhibitors show greater toxicity to some animal and human cancers than to normal cells, both clinically and in drug sensitivity tests. Such selected SH inhibitors can induce immunity against cancer in mice, unlike other commonly used antitumor agents. Scanning electron micrographs of the surface of Ehrlich ascites cancer cells treated with the sulfhydryl inhibitors show blunting to absence of microvilli and modification of the surface texture of the cancer cells.", "contents": "Selected sulfhydryl inhibitors capable of inducing immunity against cancer in mice. Selected sulfhydryl inhibitors show greater toxicity to some animal and human cancers than to normal cells, both clinically and in drug sensitivity tests. Such selected SH inhibitors can induce immunity against cancer in mice, unlike other commonly used antitumor agents. Scanning electron micrographs of the surface of Ehrlich ascites cancer cells treated with the sulfhydryl inhibitors show blunting to absence of microvilli and modification of the surface texture of the cancer cells."}
{"id": "PMID:481843", "title": "HLA system and testicular germinative tumours.", "content": "In 62 patients with testicular germinative tumours and 301 healthy unrelated subjects, 23 HLA antigens of A and B loci were tested. In the group of 40 seminomas the incidence of HLA-Bw35 antigen and in 22 patients with non-seminomas (embryonal carcinomas, teratocarcinoma and mixed forms) the frequency of HLA-A 10 antigen were significantly higher (27.50 vs. 14.28% in the controls, p greater than 0.025; 36.36 vs. 15.28%, p less than 0.025). After correction by multiplying p by the number of typed antigens there was no statistically significant result any more. The causes of dubious results of the studies about the association between HLA and malignancies are discussed.", "contents": "HLA system and testicular germinative tumours. In 62 patients with testicular germinative tumours and 301 healthy unrelated subjects, 23 HLA antigens of A and B loci were tested. In the group of 40 seminomas the incidence of HLA-Bw35 antigen and in 22 patients with non-seminomas (embryonal carcinomas, teratocarcinoma and mixed forms) the frequency of HLA-A 10 antigen were significantly higher (27.50 vs. 14.28% in the controls, p greater than 0.025; 36.36 vs. 15.28%, p less than 0.025). After correction by multiplying p by the number of typed antigens there was no statistically significant result any more. The causes of dubious results of the studies about the association between HLA and malignancies are discussed."}
{"id": "PMID:481844", "title": "Mode of spread of thyroid cancer.", "content": "Metastatic dissemination of differentiated cancer was studied in a personal group with the following results. Invasion of cancer to adjacent structures can be encountered even in children with typical increase with age. The lymphatic spread to regional lymph nodes is typical of papillary cancers and in young patients. The same type of spread without the age-dependent decrease can also be proved, with lower incidence, in follicular cancers. Pulmonary metastases are frequently the only type of distant metastases and may originate from previous spread to lymph nodes. The isolated bone metastases a;e probably brought about through the vertebral venous system. Patients having multiple bone metastases or both bone and lung lesions are probably the only typical examples of metastasizing through the systemic blood flow. As the above types of distant metastases carry different prognosis they should also be recognized by the TNM system.", "contents": "Mode of spread of thyroid cancer. Metastatic dissemination of differentiated cancer was studied in a personal group with the following results. Invasion of cancer to adjacent structures can be encountered even in children with typical increase with age. The lymphatic spread to regional lymph nodes is typical of papillary cancers and in young patients. The same type of spread without the age-dependent decrease can also be proved, with lower incidence, in follicular cancers. Pulmonary metastases are frequently the only type of distant metastases and may originate from previous spread to lymph nodes. The isolated bone metastases a;e probably brought about through the vertebral venous system. Patients having multiple bone metastases or both bone and lung lesions are probably the only typical examples of metastasizing through the systemic blood flow. As the above types of distant metastases carry different prognosis they should also be recognized by the TNM system."}
{"id": "PMID:481845", "title": "Intralesional immune therapy: methanol extraction residue of BCG or purified protein derivative.", "content": "6 patients with cutaneous malignant melanoma and multiple secondary cutaneous lesions were treated with intralesional methanol extraction residue of bacillus Calmette Guerin (MER-BCG). Separate lesions were injected with purified protein derivatives (PPD) in 5 of the study patients. 5 of the 6 MER-BCG injection lesions developed marked inflammation clinically. Excisional biopsy 7-14 days later demonstrated complete dissolution of tumor in 2 patients and was accompanied by infiltration with acute and chronic inflammatory cells; 3 lesions revealed necrosis with residual tumor, and in 1 patient there was no apparent host response. Clinical tumor regression was not observed with PPD applied intralesionally, although histopathologic analysis revealed a granulomatous inflammatory response in 3 of 5 patients. No patient demonstrated regression of uninjected cutaneous lesions (4 evaluable patients) or visceral lesions (2 patients). The critical determinants of tumor regression are the size, site and depth of the lesion in relationship to the cutaneous surface. The mechanism of tumor eradication may be related to 'innocent bystander' necrosis secondary to nonspecific inflammation rather than immunologically mediated via host sensitization.", "contents": "Intralesional immune therapy: methanol extraction residue of BCG or purified protein derivative. 6 patients with cutaneous malignant melanoma and multiple secondary cutaneous lesions were treated with intralesional methanol extraction residue of bacillus Calmette Guerin (MER-BCG). Separate lesions were injected with purified protein derivatives (PPD) in 5 of the study patients. 5 of the 6 MER-BCG injection lesions developed marked inflammation clinically. Excisional biopsy 7-14 days later demonstrated complete dissolution of tumor in 2 patients and was accompanied by infiltration with acute and chronic inflammatory cells; 3 lesions revealed necrosis with residual tumor, and in 1 patient there was no apparent host response. Clinical tumor regression was not observed with PPD applied intralesionally, although histopathologic analysis revealed a granulomatous inflammatory response in 3 of 5 patients. No patient demonstrated regression of uninjected cutaneous lesions (4 evaluable patients) or visceral lesions (2 patients). The critical determinants of tumor regression are the size, site and depth of the lesion in relationship to the cutaneous surface. The mechanism of tumor eradication may be related to 'innocent bystander' necrosis secondary to nonspecific inflammation rather than immunologically mediated via host sensitization."}
{"id": "PMID:481846", "title": "Correlation between tumour size, metastatic spread and galactosyl transferase activity in cyclophosphamide-treated mice bearing the Lewis lung carcinoma.", "content": "Galactosyl transferase activity was measured in tumour and normal tissues of mice receiving cyclophosphamide treatment for Lewis lung carcinoma. Animals which responded to cyclophosphamide therapy had significantly smaller tumours with fewer metastases than the untreated mice. The level of galactosyl transferase was significantly reduced in the tumours which were inhibited by the cyclophosphamide treatment.", "contents": "Correlation between tumour size, metastatic spread and galactosyl transferase activity in cyclophosphamide-treated mice bearing the Lewis lung carcinoma. Galactosyl transferase activity was measured in tumour and normal tissues of mice receiving cyclophosphamide treatment for Lewis lung carcinoma. Animals which responded to cyclophosphamide therapy had significantly smaller tumours with fewer metastases than the untreated mice. The level of galactosyl transferase was significantly reduced in the tumours which were inhibited by the cyclophosphamide treatment."}
{"id": "PMID:481847", "title": "[Nuclear structures in the telotrophic ovarioles of nocturnal ground beetles (Tenebrionidae, Polyphaga). II. The oocyte nucleus of Blaps lethifera and Gnaptor spinimanus. Light optical data].", "content": "Changes in the nuclear structures and their participation in RNA synthesis in the growing oocytes were followed in two species of beetles Blaps lethifera and Gnaptor spinimanus. In the oocytes of both the species, the chromosomes join into the karyosphere following the short-term lampbrush stage. A large capsule appears around the karayosphere which consists of the fibrous substance, granules and karyosphere nucleoli. The latter form in the karyosphere and contain RNP but they are not true nucleoli since they do not include 3H-uridine. RNA synthesis on the chromosomes, active at the lampbrush stage, falls markedly following their joining into the karyosphere. The oocyte nuclei of these beetles are, thus, characterized by the absence of RNA synthesizing nucleolar system and, as compared with the trophocytes, by the low level of RNA synthesis on the chromosomes.", "contents": "[Nuclear structures in the telotrophic ovarioles of nocturnal ground beetles (Tenebrionidae, Polyphaga). II. The oocyte nucleus of Blaps lethifera and Gnaptor spinimanus. Light optical data]. Changes in the nuclear structures and their participation in RNA synthesis in the growing oocytes were followed in two species of beetles Blaps lethifera and Gnaptor spinimanus. In the oocytes of both the species, the chromosomes join into the karyosphere following the short-term lampbrush stage. A large capsule appears around the karayosphere which consists of the fibrous substance, granules and karyosphere nucleoli. The latter form in the karyosphere and contain RNP but they are not true nucleoli since they do not include 3H-uridine. RNA synthesis on the chromosomes, active at the lampbrush stage, falls markedly following their joining into the karyosphere. The oocyte nuclei of these beetles are, thus, characterized by the absence of RNA synthesizing nucleolar system and, as compared with the trophocytes, by the low level of RNA synthesis on the chromosomes."}
{"id": "PMID:481848", "title": "[Nuclear structures in the telotrophic ovarioles of nocturnal ground beetles (Tenebrionidae, Polyphaga). III. The oocyte nucleus. Electron microscopic data].", "content": "The fine structural organization of nuclei was studied in the growing oocytes of Blaps lethifera, B. mortisaga and Gnaptor spinimanus. In the beginning of diplotene the nuclei contain primary fibrillar nucleoli and numerous electron dense globules dispersed all over the nucleus; the loose chromosome material (lampbrush chromosomes) is distributed all over the nucleus. With the oocyte growth the chromosomes are spiralized and join into the karyosphere. A capsule of fibrous material forms around the karyosphere. The karyosphere nucleoli appear on the chromosomes and, then, move to the capsule region and outside its limits, to the nuclear envelope. They are fibrillar and non-active with respect to RNA synthesis. The fibrous material of the capsule is represented by strands which consist of bundles of cross-striated filaments. These latter contact directly with the chromosomes in the karyosphere and with the surface of the karyosphere nucleoli. The fibrillar-granular bodies are distributed along the strands in the capsule; they contain both RNA and DNA. The nature of extrachromosomal DNA in the karyosphere capsule and its participation in the formation of the capsule material are discussed. A suggestion is put forward on the similarity of the capsule strands with the modified central elements of synaptinemal complex.", "contents": "[Nuclear structures in the telotrophic ovarioles of nocturnal ground beetles (Tenebrionidae, Polyphaga). III. The oocyte nucleus. Electron microscopic data]. The fine structural organization of nuclei was studied in the growing oocytes of Blaps lethifera, B. mortisaga and Gnaptor spinimanus. In the beginning of diplotene the nuclei contain primary fibrillar nucleoli and numerous electron dense globules dispersed all over the nucleus; the loose chromosome material (lampbrush chromosomes) is distributed all over the nucleus. With the oocyte growth the chromosomes are spiralized and join into the karyosphere. A capsule of fibrous material forms around the karyosphere. The karyosphere nucleoli appear on the chromosomes and, then, move to the capsule region and outside its limits, to the nuclear envelope. They are fibrillar and non-active with respect to RNA synthesis. The fibrous material of the capsule is represented by strands which consist of bundles of cross-striated filaments. These latter contact directly with the chromosomes in the karyosphere and with the surface of the karyosphere nucleoli. The fibrillar-granular bodies are distributed along the strands in the capsule; they contain both RNA and DNA. The nature of extrachromosomal DNA in the karyosphere capsule and its participation in the formation of the capsule material are discussed. A suggestion is put forward on the similarity of the capsule strands with the modified central elements of synaptinemal complex."}
{"id": "PMID:481849", "title": "[Characteristics of oogenesis in the chick. I. The extrafollicular period in the development of the oocytes].", "content": "The early stages of oogenesis in the chick were studied by means of light cytochemistry and autoradiography. The oocytes devoided of follicular epithelium are located in the ovarian cortex in groups of 8, 16 or 32 cells. Within the bounds of the group they develop synchronously until the beginning of formation of the follicular epithelium around each oocyte. By 15 days of postembryonic development all oocytes in the ovarian cortex enter the diplotene stage of meiotic prophase. The formation of follicles is completed by 22 days. The morphological descriptions are given for the oocyte nuclei at the preleptotene, leptotene, zygotene, pachytene and early diplotene stages of meiotic prophase. The nucleolus appears at the early diplotene stage and is characterized by extremely weak synthetic activity. It is specially stressed that the nucleolus forms not in alloocytes and that the population of germ cells in the chick ovary is heterogenous by this character. The beginning of RNA synthesis in the oocyte nucleus is timed to the early diplotene stage. The data are obtained on DNA synthesis in the nuclei of preleptotene and leptotene oocytes not related to the amplification of ribosomal genes.", "contents": "[Characteristics of oogenesis in the chick. I. The extrafollicular period in the development of the oocytes]. The early stages of oogenesis in the chick were studied by means of light cytochemistry and autoradiography. The oocytes devoided of follicular epithelium are located in the ovarian cortex in groups of 8, 16 or 32 cells. Within the bounds of the group they develop synchronously until the beginning of formation of the follicular epithelium around each oocyte. By 15 days of postembryonic development all oocytes in the ovarian cortex enter the diplotene stage of meiotic prophase. The formation of follicles is completed by 22 days. The morphological descriptions are given for the oocyte nuclei at the preleptotene, leptotene, zygotene, pachytene and early diplotene stages of meiotic prophase. The nucleolus appears at the early diplotene stage and is characterized by extremely weak synthetic activity. It is specially stressed that the nucleolus forms not in alloocytes and that the population of germ cells in the chick ovary is heterogenous by this character. The beginning of RNA synthesis in the oocyte nucleus is timed to the early diplotene stage. The data are obtained on DNA synthesis in the nuclei of preleptotene and leptotene oocytes not related to the amplification of ribosomal genes."}
{"id": "PMID:481850", "title": "[Cell proliferation and migration in the roof of the mesencephalon (tectum) in Xenopus laevis tadpoles and adult frogs normally and in brain injury. II. Cell proliferation and differentiation of the tectum in frogs].", "content": "The proliferation and directions of cell differentiation in tectum opticum were studied in the young frogs under the conditions of normal development and upon brain trauma by means of 3H-thymidine autoradiography. The same types of cells were shown to be able of proliferation in both the cases: cells of the ventricle zone and glioblasts (gliocytes) in all other tectum layers. A study of directions of the tectum proliferating cells' differentiation in the frogs has shown that the proliferating cells differentiate mainly in the ependyme tanicytes in the ventricle layer 1 and gliocytes in the other tectum layers. The trauma did not change the direction of proliferating cells' differentiation towards the formation of neurons. The complete regeneration is observed in the tectum layer 1 only.", "contents": "[Cell proliferation and migration in the roof of the mesencephalon (tectum) in Xenopus laevis tadpoles and adult frogs normally and in brain injury. II. Cell proliferation and differentiation of the tectum in frogs]. The proliferation and directions of cell differentiation in tectum opticum were studied in the young frogs under the conditions of normal development and upon brain trauma by means of 3H-thymidine autoradiography. The same types of cells were shown to be able of proliferation in both the cases: cells of the ventricle zone and glioblasts (gliocytes) in all other tectum layers. A study of directions of the tectum proliferating cells' differentiation in the frogs has shown that the proliferating cells differentiate mainly in the ependyme tanicytes in the ventricle layer 1 and gliocytes in the other tectum layers. The trauma did not change the direction of proliferating cells' differentiation towards the formation of neurons. The complete regeneration is observed in the tectum layer 1 only."}
{"id": "PMID:481851", "title": "[Protein functional groups of the ova in the roach and carp-bream of varying degrees of maturity].", "content": "The Black Sea roach and carp-bream eggs of different degree of maturity differ from each other by the content of SH-, S--S-groups of proteins and non-protein low molecular weight thiol compounds. Definite differences were found in the optical density of soluble proteins and degree of ionization of their side groups.", "contents": "[Protein functional groups of the ova in the roach and carp-bream of varying degrees of maturity]. The Black Sea roach and carp-bream eggs of different degree of maturity differ from each other by the content of SH-, S--S-groups of proteins and non-protein low molecular weight thiol compounds. Definite differences were found in the optical density of soluble proteins and degree of ionization of their side groups."}
{"id": "PMID:481852", "title": "[3H-thymidine incorporation into the macrophages in the process of eye regeneration in adult tritons].", "content": "Changes in the number of labelled macrophages in the regenerating eye cavity of the adult common newts were studied within 2 to 12 days following the injection of 3H-thymidine and removal of retina and lens from the eyes. A small number of labelled macrophages was found in the eye cavity (0,2--5.9%) at all regeneration stages under study upon the pulse 3H-thymidine incorporation. Their number rapidly increased and attained by the end of observation (12 days) 73%. These results suggest that mainly mature non-dividing forms of macrophages which completed the mitotic cycle S-phase in the places of their active reproduction migrate in the eye cavity. The sharp increase of the number of labelled macrophages in the eye cavity is determined by the migration of those macrophages the precursors of which were labelled as a result of both the pulse 3H-thymidine incorporation and reutilization of the labelled precursors of DNA synthesis. New portions of labelled macrophages migrate in the eye cavity within 2 to 4 days.", "contents": "[3H-thymidine incorporation into the macrophages in the process of eye regeneration in adult tritons]. Changes in the number of labelled macrophages in the regenerating eye cavity of the adult common newts were studied within 2 to 12 days following the injection of 3H-thymidine and removal of retina and lens from the eyes. A small number of labelled macrophages was found in the eye cavity (0,2--5.9%) at all regeneration stages under study upon the pulse 3H-thymidine incorporation. Their number rapidly increased and attained by the end of observation (12 days) 73%. These results suggest that mainly mature non-dividing forms of macrophages which completed the mitotic cycle S-phase in the places of their active reproduction migrate in the eye cavity. The sharp increase of the number of labelled macrophages in the eye cavity is determined by the migration of those macrophages the precursors of which were labelled as a result of both the pulse 3H-thymidine incorporation and reutilization of the labelled precursors of DNA synthesis. New portions of labelled macrophages migrate in the eye cavity within 2 to 4 days."}
{"id": "PMID:481853", "title": "[Heat resistance of the eggs of a mulberry silkworm strain adapted to a subtropical climate].", "content": "The heat stability of the eggs of a polyvoltine strain of Bombyx mori occurring in the conditions of hot climate was studied. No marked differences between this strain and the moderate climate strains were found in the level of heat stability and the character of its age changes. The mechanisms of heat stability upon the diapause-less egg development are discussed.", "contents": "[Heat resistance of the eggs of a mulberry silkworm strain adapted to a subtropical climate]. The heat stability of the eggs of a polyvoltine strain of Bombyx mori occurring in the conditions of hot climate was studied. No marked differences between this strain and the moderate climate strains were found in the level of heat stability and the character of its age changes. The mechanisms of heat stability upon the diapause-less egg development are discussed."}
{"id": "PMID:481854", "title": "[Functional state of the thyroid gland in the postnatal period in mink of different genotypes].", "content": "The thyroid gland functional state was studied by means of 131J-triiodothyronine in minks of two genotypes. The thyroid activity in the standard (dark brown) minks increased from the 3--4th month of postnatal ontogenesis (July--August) and decreased gradually by the 6th month (november). Similar changes in the thyroid activity were found in the Hedlung (white) minks as well. Their thyroid activity was, however, markedly higher on the 3rd month than in the standard minks.", "contents": "[Functional state of the thyroid gland in the postnatal period in mink of different genotypes]. The thyroid gland functional state was studied by means of 131J-triiodothyronine in minks of two genotypes. The thyroid activity in the standard (dark brown) minks increased from the 3--4th month of postnatal ontogenesis (July--August) and decreased gradually by the 6th month (november). Similar changes in the thyroid activity were found in the Hedlung (white) minks as well. Their thyroid activity was, however, markedly higher on the 3rd month than in the standard minks."}
{"id": "PMID:481855", "title": "Peripheral iridectomy via a corneal section: a follow-up study.", "content": "A method of performing peripheral iridectomy through a corneal incision is described. Of the fifty patients reviewed, there were no immediate postoperative complications. In the follow-up period the incidence of lens opacities associated with visual loss was 16% and 21% in the prophylactic and therapeutic groups respectively. The percentage of posterior synechiae in the prophylactic group was 16%. Ninety-three percent of patients had a visual acuity of 6/12 or better at the end of the follow-up period. When compared with the results of previous surveys, the method is as safe as the ab externo approach. The technique avoids bleeding and leaves the bulbar conjunctiva undamaged and available for any subsequent drainage procedure.", "contents": "Peripheral iridectomy via a corneal section: a follow-up study. A method of performing peripheral iridectomy through a corneal incision is described. Of the fifty patients reviewed, there were no immediate postoperative complications. In the follow-up period the incidence of lens opacities associated with visual loss was 16% and 21% in the prophylactic and therapeutic groups respectively. The percentage of posterior synechiae in the prophylactic group was 16%. Ninety-three percent of patients had a visual acuity of 6/12 or better at the end of the follow-up period. When compared with the results of previous surveys, the method is as safe as the ab externo approach. The technique avoids bleeding and leaves the bulbar conjunctiva undamaged and available for any subsequent drainage procedure."}
{"id": "PMID:481857", "title": "Coated Vicryl (polyglactin 910) suture in extraocular muscle surgery.", "content": "A newly available coated Vicryl (Polyglactin 910) suture was evaluated in laboratory and clinical trials for use in extraocular muscle surgery. The suture retains the increased strength and low antigenicity of uncoated Vicryl suture, while providing easier passage through tissue and improved tying characteristics. The improved handling properties result from the coating which produces a smoother suture surface.", "contents": "Coated Vicryl (polyglactin 910) suture in extraocular muscle surgery. A newly available coated Vicryl (Polyglactin 910) suture was evaluated in laboratory and clinical trials for use in extraocular muscle surgery. The suture retains the increased strength and low antigenicity of uncoated Vicryl suture, while providing easier passage through tissue and improved tying characteristics. The improved handling properties result from the coating which produces a smoother suture surface."}
{"id": "PMID:481858", "title": "Complications of 10-0 nylon sutures.", "content": "Three cases are presented which document the finding that 10-0 monofilament nylon suture may cut through corneal tissue after a coup or contrecoup injury to a recently operated eye. The authors speculate that a larger gauge suture does not have the tendency to cut through recently operated corneal tissue as easily as 10-0 monifilament nylon. The authors recommend the use of a larger gauge suture in closing corneal incisions in persons whom the surgeon feels are at a greater postoperative traumatic risk.", "contents": "Complications of 10-0 nylon sutures. Three cases are presented which document the finding that 10-0 monofilament nylon suture may cut through corneal tissue after a coup or contrecoup injury to a recently operated eye. The authors speculate that a larger gauge suture does not have the tendency to cut through recently operated corneal tissue as easily as 10-0 monifilament nylon. The authors recommend the use of a larger gauge suture in closing corneal incisions in persons whom the surgeon feels are at a greater postoperative traumatic risk."}
{"id": "PMID:481859", "title": "Praeger micro irrigating hook for intraocular lens implantation.", "content": "A new micro blunt irrigating hook is introduced for utilization in intraocular lens implant  surgery. It is simple, inexpensive, and requires no previous experience. It allows for adequate reposition of the superior loop of any type of lens along with the recovery of the proximal loop of a McCannel suture. Finally, it allows the most inexperienced operator to center a lens ideally in a totally deep chamber, avoiding the vicissitudes of either perforation of the posterior capsule, anterior hyloid, or damage to the delicate endothelium.", "contents": "Praeger micro irrigating hook for intraocular lens implantation. A new micro blunt irrigating hook is introduced for utilization in intraocular lens implant  surgery. It is simple, inexpensive, and requires no previous experience. It allows for adequate reposition of the superior loop of any type of lens along with the recovery of the proximal loop of a McCannel suture. Finally, it allows the most inexperienced operator to center a lens ideally in a totally deep chamber, avoiding the vicissitudes of either perforation of the posterior capsule, anterior hyloid, or damage to the delicate endothelium."}
{"id": "PMID:481860", "title": "Praeger irrigating micro intraocular scissors.", "content": "The Praeger Irrigating Micro Mini Irrigating Scissors is a valuable instrument particularly in phacoemulsification with or without intraoucular lens implantation and when used with microscopy. Secondary procedures such as a posterior capsulotomy may be safely carried out on very dense posterior capsules either beneath a pseudophakosis or following extracapsular cataract extraction. Inferior sphincterotomies through the superior incision and resection of anterior capsule through a 3.0-mm incision during the course of phacoemulsification can be easily accomplished without fear of perforation to the posterior capsule or damage to the overlying corneal endothelium. Additional intraocular manipulations such as direct observation and resection of a McCannel suture in the lower 6 o'clock meridian can be accomplished under direct visualization without excessive traction on the suture.", "contents": "Praeger irrigating micro intraocular scissors. The Praeger Irrigating Micro Mini Irrigating Scissors is a valuable instrument particularly in phacoemulsification with or without intraoucular lens implantation and when used with microscopy. Secondary procedures such as a posterior capsulotomy may be safely carried out on very dense posterior capsules either beneath a pseudophakosis or following extracapsular cataract extraction. Inferior sphincterotomies through the superior incision and resection of anterior capsule through a 3.0-mm incision during the course of phacoemulsification can be easily accomplished without fear of perforation to the posterior capsule or damage to the overlying corneal endothelium. Additional intraocular manipulations such as direct observation and resection of a McCannel suture in the lower 6 o'clock meridian can be accomplished under direct visualization without excessive traction on the suture."}
{"id": "PMID:481863", "title": "Excretion of vanilmandelic acid in the urine of retinoblastoma patients.", "content": "The idea of determining the urinary excretion of vanilmandelic acid (VMA) in children with retinoblastoma derives from the fact that elevated amounts of VMA were found in children with neurogenic tumours and especially neuroblastoma. The method of VMA analysis and the results are discussed. In view of the fact that in only 5 out of our 12 patients an elevated urinary VMA excretion was found, we cannot recommend this determination as a routine diagnostic procedure. However, further research in this direction is necessary.", "contents": "Excretion of vanilmandelic acid in the urine of retinoblastoma patients. The idea of determining the urinary excretion of vanilmandelic acid (VMA) in children with retinoblastoma derives from the fact that elevated amounts of VMA were found in children with neurogenic tumours and especially neuroblastoma. The method of VMA analysis and the results are discussed. In view of the fact that in only 5 out of our 12 patients an elevated urinary VMA excretion was found, we cannot recommend this determination as a routine diagnostic procedure. However, further research in this direction is necessary."}
{"id": "PMID:481864", "title": "Malignant epithelioma of the ciliary body.", "content": "A rare case of malignant epithelioma of the ciliary body (verified histologically) is described. Some difficulties of clinical diagnosis are mentioned as well as the general properties of this kind of tumor, which mostly requires an anatomical investigation for recognition. As a special feature of this kind of tumor the precocious consecutive cataract is mentioned; this arises in cases of melanomas of the ciliary body considerably later in the course of malignant growth.", "contents": "Malignant epithelioma of the ciliary body. A rare case of malignant epithelioma of the ciliary body (verified histologically) is described. Some difficulties of clinical diagnosis are mentioned as well as the general properties of this kind of tumor, which mostly requires an anatomical investigation for recognition. As a special feature of this kind of tumor the precocious consecutive cataract is mentioned; this arises in cases of melanomas of the ciliary body considerably later in the course of malignant growth."}
{"id": "PMID:481865", "title": "Effects of urea treatment in malignancies of the conjunctiva and cornea.", "content": "A series of 9 patients with extensive squamous cell carcinomas of the conjunctiva, affecting also the cornea in 5 of them, were treated with local applications of urea. 8 out of these patients were cured. In 1 patient with a concomitant extensive conjunctivitis, the treatment was ineffective. Urea treatment is very simple and without any complications, apart from a transient opacity of the cornea. Recurrences are easily and effectively retreated as in 1 of 2 of our cases. But if the eyeball has been destroyed, as in the second case, retreatment is impossible. At least 5 out of the treated patients would have had an enucleation of the globe or an exenteration of the orbit without the urea treatment.", "contents": "Effects of urea treatment in malignancies of the conjunctiva and cornea. A series of 9 patients with extensive squamous cell carcinomas of the conjunctiva, affecting also the cornea in 5 of them, were treated with local applications of urea. 8 out of these patients were cured. In 1 patient with a concomitant extensive conjunctivitis, the treatment was ineffective. Urea treatment is very simple and without any complications, apart from a transient opacity of the cornea. Recurrences are easily and effectively retreated as in 1 of 2 of our cases. But if the eyeball has been destroyed, as in the second case, retreatment is impossible. At least 5 out of the treated patients would have had an enucleation of the globe or an exenteration of the orbit without the urea treatment."}
{"id": "PMID:481866", "title": "[Clinical experiences with timolol eye drops].", "content": "Three groups of chronic simple glaucoma (group I: newly adjusted patients; group II: previously satisfactorily pressure-regulated patients; group III: previously notsatisfactorily pressure-regulated patients) received treatment with Timolol eye drops for 4 months. In 34 eyes the intra-ocular pressure could be lowered below 22 mm Hg by Timolol alone, whereas 10 eyes additionally had to be treated with 1% epinephrine. The higher the initial pressure effect, the more pronounced the pressure-lowering properties of Timolol became. Especially in the first 2 weeks, less in the following 14 weeks, Timolol showed a loss in effectiveness. The application of Timolol instead of carbonic anhydrase inhibitors with acute angle-closure glaucoma and secondary glaucoma was successful. Timolol eye drops were tolerated very well. There were no local or systemic side effects.", "contents": "[Clinical experiences with timolol eye drops]. Three groups of chronic simple glaucoma (group I: newly adjusted patients; group II: previously satisfactorily pressure-regulated patients; group III: previously notsatisfactorily pressure-regulated patients) received treatment with Timolol eye drops for 4 months. In 34 eyes the intra-ocular pressure could be lowered below 22 mm Hg by Timolol alone, whereas 10 eyes additionally had to be treated with 1% epinephrine. The higher the initial pressure effect, the more pronounced the pressure-lowering properties of Timolol became. Especially in the first 2 weeks, less in the following 14 weeks, Timolol showed a loss in effectiveness. The application of Timolol instead of carbonic anhydrase inhibitors with acute angle-closure glaucoma and secondary glaucoma was successful. Timolol eye drops were tolerated very well. There were no local or systemic side effects."}
{"id": "PMID:481867", "title": "Retinal function after surgical treatment of detachment.", "content": "1--8 years after successful surgery, for retinal detachment 47 patients (48 eyes) were included in the study. By using Goldmann's static perimetry macular and retinal periphery sensitivities were determined (in 2 meridians) as compared with the healthy eye. It was established that the changes in static perimetry run a course parallel to the disturbances in the perception of the Haidinger phenomenon and poor performance at the Amsler tests. The longer the period of macular detachment was, the greater were the changes in its sensitivity curve despite good visual acuity. A distinct lowering in sensitivity occurs also outside the macula, all over the area in which retinal detachment had occurred.", "contents": "Retinal function after surgical treatment of detachment. 1--8 years after successful surgery, for retinal detachment 47 patients (48 eyes) were included in the study. By using Goldmann's static perimetry macular and retinal periphery sensitivities were determined (in 2 meridians) as compared with the healthy eye. It was established that the changes in static perimetry run a course parallel to the disturbances in the perception of the Haidinger phenomenon and poor performance at the Amsler tests. The longer the period of macular detachment was, the greater were the changes in its sensitivity curve despite good visual acuity. A distinct lowering in sensitivity occurs also outside the macula, all over the area in which retinal detachment had occurred."}
{"id": "PMID:481868", "title": "[Echographic differential diagnosis of leukokorie in childhood].", "content": "After the description of the most important differential diagnoses of leukocoria in childhood, their ultrasonographical findings in both A- and B-mode are discussed. Ultrasound proofs to be a useful aid in the evaluation of leukocoria.", "contents": "[Echographic differential diagnosis of leukokorie in childhood]. After the description of the most important differential diagnoses of leukocoria in childhood, their ultrasonographical findings in both A- and B-mode are discussed. Ultrasound proofs to be a useful aid in the evaluation of leukocoria."}
{"id": "PMID:481869", "title": "Change in oscillatory potential by thioridazine.", "content": "Toxic effects on the retina by oral administration of thioridazine was investigated by electroretinography (ERG) using male Wistar rats. An obvious reduction followed by a disappearance in the O2 component of the oscillatory potentials of the ERG was seen in a dose-dependent manner. The minimum dosage given to produce the above change was 3 mg/kg/day for 7 days' administration. This change seemed to be permanent, whereas a and b waves as well as other oscillatory potentials, e.g., O1, O3, were normal throughout the experiment. The significance of the data is discussed.", "contents": "Change in oscillatory potential by thioridazine. Toxic effects on the retina by oral administration of thioridazine was investigated by electroretinography (ERG) using male Wistar rats. An obvious reduction followed by a disappearance in the O2 component of the oscillatory potentials of the ERG was seen in a dose-dependent manner. The minimum dosage given to produce the above change was 3 mg/kg/day for 7 days' administration. This change seemed to be permanent, whereas a and b waves as well as other oscillatory potentials, e.g., O1, O3, were normal throughout the experiment. The significance of the data is discussed."}
{"id": "PMID:481870", "title": "Diffuse malignant ring melanoma of the iris and ciliary body.", "content": "2 cases of diffuse malignant ring melanoma of the anterior uvea are presented. A correct clinical diagnosis led to enucleation in case 1, while in case 2, the eye was enucleated 2 years after anti-glaucomatous surgery.", "contents": "Diffuse malignant ring melanoma of the iris and ciliary body. 2 cases of diffuse malignant ring melanoma of the anterior uvea are presented. A correct clinical diagnosis led to enucleation in case 1, while in case 2, the eye was enucleated 2 years after anti-glaucomatous surgery."}
{"id": "PMID:481871", "title": "Polymers produced by heating an amino acid mixture in sea water enriched with transition elements.", "content": "A mixture of eighteen protein amino acids was heated in sea water medium enriched with transition metal ions. Small granules were obtained as precipitates. Both dialyzable polymers and undialyzable polymers were obtained from the supernatant. Dialyzable polymers yielded mainly Glu, Asp, Ser, and Thr on hydrolysis; undialyzable polymers (C, 29.45; H, 3.87; N, 4.87; and ash, 31.5 wt%) yielded Thr, Asp, Glu, Gly, Leu, Ser, Lys, Pro, His, Phe, and a few unidentified ninhydrin positive peaks after acid hydrolysis. Five wt% of the undialyzable polymers con-ist of acid-hydrolyzable protein amino acids.", "contents": "Polymers produced by heating an amino acid mixture in sea water enriched with transition elements. A mixture of eighteen protein amino acids was heated in sea water medium enriched with transition metal ions. Small granules were obtained as precipitates. Both dialyzable polymers and undialyzable polymers were obtained from the supernatant. Dialyzable polymers yielded mainly Glu, Asp, Ser, and Thr on hydrolysis; undialyzable polymers (C, 29.45; H, 3.87; N, 4.87; and ash, 31.5 wt%) yielded Thr, Asp, Glu, Gly, Leu, Ser, Lys, Pro, His, Phe, and a few unidentified ninhydrin positive peaks after acid hydrolysis. Five wt% of the undialyzable polymers con-ist of acid-hydrolyzable protein amino acids."}
{"id": "PMID:481872", "title": "Mirror symmetry breaking in biochemical evolution.", "content": "The problem of origination of molecular asymmetry in biochemical evolution is discussed. The theoretical analysis shows that chiral purity of biomolecules has the biological significance for self-reproduction of organisms. The models of spontaneous symmetry-breaking in molecular systems are given. The aspects of various stages of biochemical evolution associated with the development of chiral polarization are analysed.", "contents": "Mirror symmetry breaking in biochemical evolution. The problem of origination of molecular asymmetry in biochemical evolution is discussed. The theoretical analysis shows that chiral purity of biomolecules has the biological significance for self-reproduction of organisms. The models of spontaneous symmetry-breaking in molecular systems are given. The aspects of various stages of biochemical evolution associated with the development of chiral polarization are analysed."}
{"id": "PMID:481873", "title": "On the physical origin of biological handedness.", "content": "In the racemic conglomerate crystallization of over 1,000 samples of D,L-sodium-ammonium tartrate the effect of 32P beta irradiation on the weight, optical activity, and crystallite size was measured. Both weight and optical activity showed a statistical dependence on the intensity of beta irradiation. The crystallite size is also affected by the presence of 32P. Asymmetric crystals are suggested to have been potential mediators between asymmetric parity violating forces and molecular asymmetry so that stereo-selective prebiotic chemical reactions involving crystals need not be considered 'chance' processes. No measurable difference in the energy content of optical isomers was found. An upper limit for the direct contribution of weak interactions to electromagnetic ones has been calculated. The mechanism of stereoselective crystal seeding by beta particles is discussed.", "contents": "On the physical origin of biological handedness. In the racemic conglomerate crystallization of over 1,000 samples of D,L-sodium-ammonium tartrate the effect of 32P beta irradiation on the weight, optical activity, and crystallite size was measured. Both weight and optical activity showed a statistical dependence on the intensity of beta irradiation. The crystallite size is also affected by the presence of 32P. Asymmetric crystals are suggested to have been potential mediators between asymmetric parity violating forces and molecular asymmetry so that stereo-selective prebiotic chemical reactions involving crystals need not be considered 'chance' processes. No measurable difference in the energy content of optical isomers was found. An upper limit for the direct contribution of weak interactions to electromagnetic ones has been calculated. The mechanism of stereoselective crystal seeding by beta particles is discussed."}
{"id": "PMID:481874", "title": "Solar-driven chemical energy source for a Martian biota.", "content": "Microorganisms deep in the Martian soil could derive energy indirectly from the sun via chemical reactions involving atmospheric products of the solar ultraviolet flux. The Viking discovery of a chemically uniform regolith which, though poor in organics, is rich in sulfur-containing compounds suggests reaction sequences in which sulfur is recycled through reduced and oxidized states by biologically catalyzed reactions with photochemically-produced atmospheric constituents. One candidate reaction, reduction of soil ssufate minerals by molecular hydrogen, is already exploited on earth by bacteria of the ubiquitous and tenacious Desulfovibrio genus.", "contents": "Solar-driven chemical energy source for a Martian biota. Microorganisms deep in the Martian soil could derive energy indirectly from the sun via chemical reactions involving atmospheric products of the solar ultraviolet flux. The Viking discovery of a chemically uniform regolith which, though poor in organics, is rich in sulfur-containing compounds suggests reaction sequences in which sulfur is recycled through reduced and oxidized states by biologically catalyzed reactions with photochemically-produced atmospheric constituents. One candidate reaction, reduction of soil ssufate minerals by molecular hydrogen, is already exploited on earth by bacteria of the ubiquitous and tenacious Desulfovibrio genus."}
{"id": "PMID:481875", "title": "Some comments on interpretations of Viking biological experiments.", "content": "Data from Viking experiments are analyzed from a biological viewpoint. The lack of organic matter in Martian soil could be due to the specificity of regions with optimal landing conditions. All data of labeled release experiments may be explained by assuming the existence of psychrophilic organisms, cultivated at temperatures above optimal ones, and other factors of a biological origin. The biological interpretation does not contradict the results of other life search experiments.", "contents": "Some comments on interpretations of Viking biological experiments. Data from Viking experiments are analyzed from a biological viewpoint. The lack of organic matter in Martian soil could be due to the specificity of regions with optimal landing conditions. All data of labeled release experiments may be explained by assuming the existence of psychrophilic organisms, cultivated at temperatures above optimal ones, and other factors of a biological origin. The biological interpretation does not contradict the results of other life search experiments."}
{"id": "PMID:481897", "title": "[Change in the content of inorganic ions in the hemolymph of freshwater mollusks infected with trematode parthenitae].", "content": "Changes in the concentration of ions of sodium, potassium, calcium and chlorine in the haemolymph of Lymnaea stagnalis and Planorbis corneus were studied during their infection with sporocysts and rediae of trematodes. Statistically reliable disturbances in the ion balance caused by the infection were established.", "contents": "[Change in the content of inorganic ions in the hemolymph of freshwater mollusks infected with trematode parthenitae]. Changes in the concentration of ions of sodium, potassium, calcium and chlorine in the haemolymph of Lymnaea stagnalis and Planorbis corneus were studied during their infection with sporocysts and rediae of trematodes. Statistically reliable disturbances in the ion balance caused by the infection were established."}
{"id": "PMID:481904", "title": "Two new species of Lepeophtheirus (Copepoda: Caligidae) parasitic on fishes from southern California waters.", "content": "Two new species of Lepeophtheirus are described and illustrated based on a collection from the Orange County sewer outfall, California. They are: L. remiopsis sp.nov. from the branchial cavities and external body surfaces of Parophrys vetulus Girard, Hippoglossina stomata Eigenmann & Eigenmann, Pleuronichthys verticalis (Jordan & Gilbert), Chitonotus pugetensis (Steindachner), and Porichthys notatus (Girard); and L. rotundipes sp.nov. from the external body surfaces and occasionally the gill cavities of Scorpaena guttata (Girard) and Citharichthys stigmaeus Jordan & Gilbert.", "contents": "Two new species of Lepeophtheirus (Copepoda: Caligidae) parasitic on fishes from southern California waters. Two new species of Lepeophtheirus are described and illustrated based on a collection from the Orange County sewer outfall, California. They are: L. remiopsis sp.nov. from the branchial cavities and external body surfaces of Parophrys vetulus Girard, Hippoglossina stomata Eigenmann & Eigenmann, Pleuronichthys verticalis (Jordan & Gilbert), Chitonotus pugetensis (Steindachner), and Porichthys notatus (Girard); and L. rotundipes sp.nov. from the external body surfaces and occasionally the gill cavities of Scorpaena guttata (Girard) and Citharichthys stigmaeus Jordan & Gilbert."}
{"id": "PMID:481898", "title": "[Phosphoenolpyruvate carboxykinase in Calicophoron erschowi (Trematoda: Paramphistomidae)].", "content": "The trematode Calicophoron erschowi possesses high active phosphoenolpyruvate carboxykinase. Enzyme activity is concentrated in the cytoplasm. The enzyme has the optimum pH 6.0 and is active in the presence of ions Mn++ and inosine diphosphate. Alanine does not affect its activity. Sulphide oxinide in concentration 1.4-10(-6) M inhibits the activity up to 93%. Some kinetic characteristics are presented.", "contents": "[Phosphoenolpyruvate carboxykinase in Calicophoron erschowi (Trematoda: Paramphistomidae)]. The trematode Calicophoron erschowi possesses high active phosphoenolpyruvate carboxykinase. Enzyme activity is concentrated in the cytoplasm. The enzyme has the optimum pH 6.0 and is active in the presence of ions Mn++ and inosine diphosphate. Alanine does not affect its activity. Sulphide oxinide in concentration 1.4-10(-6) M inhibits the activity up to 93%. Some kinetic characteristics are presented."}
{"id": "PMID:481905", "title": "Bovine trypanosomiasis: the red cell kinetics of ndama and Zebu cattle infected with Trypanosoma congolense.", "content": "The responses of susceptible Ndama and Zebu cattle to needle challenge with Trypanosoma congolense were followed using parasitological, haematological and radio-isotopic methods and compared with those of corresponding uninfected animals. In both breeds, infection became patent at the same time but peak parasitaemias were significantly lower, were attained later and were of short duration in the Ndama. All infected animals became anaemic, the severity of which correlated with the level and duration of parasitaemia. However, even when parasites could no longer be detected in the blood, packed cell volumes showed little tendency to recover. The anaemia was due to increased intravascular red cell destruction and was more pronounced in the Zebu. Haemodilution was not a feature. Increased red cell syntheisis occurred in infected animals of both breeds but particularly in the Zebu; this accounted for the capacity to maintain packed cell volume levels following the initial drop associated with parasitaemia. However, in most cases red cell synthesis was less than expected from the degree of anaemia, suggesting impairment of bone marrow function. Measurement of red cell iron utilization indicated that this was due to effective from re-utilization from degraded red cells arising from reticulo-endothelial blockade. It is concluded that the anaemia in this disease and its underlying processes are broadly in line with the number of parasites in the blood and that the superior resistance of the Ndama cattle lies in their ability to control parasitaemia rather than their capacity to mount a more efficient erythropoietic response.", "contents": "Bovine trypanosomiasis: the red cell kinetics of ndama and Zebu cattle infected with Trypanosoma congolense. The responses of susceptible Ndama and Zebu cattle to needle challenge with Trypanosoma congolense were followed using parasitological, haematological and radio-isotopic methods and compared with those of corresponding uninfected animals. In both breeds, infection became patent at the same time but peak parasitaemias were significantly lower, were attained later and were of short duration in the Ndama. All infected animals became anaemic, the severity of which correlated with the level and duration of parasitaemia. However, even when parasites could no longer be detected in the blood, packed cell volumes showed little tendency to recover. The anaemia was due to increased intravascular red cell destruction and was more pronounced in the Zebu. Haemodilution was not a feature. Increased red cell syntheisis occurred in infected animals of both breeds but particularly in the Zebu; this accounted for the capacity to maintain packed cell volume levels following the initial drop associated with parasitaemia. However, in most cases red cell synthesis was less than expected from the degree of anaemia, suggesting impairment of bone marrow function. Measurement of red cell iron utilization indicated that this was due to effective from re-utilization from degraded red cells arising from reticulo-endothelial blockade. It is concluded that the anaemia in this disease and its underlying processes are broadly in line with the number of parasites in the blood and that the superior resistance of the Ndama cattle lies in their ability to control parasitaemia rather than their capacity to mount a more efficient erythropoietic response."}
{"id": "PMID:481900", "title": "[Fleas of small mammals in northern Amur Province and their distribution by biotopes (Aphaniptera)].", "content": "In June-September of 1975 on the southern slope of the Stanovoy ridge, in the region of construction of the Baikal-Amur main line 2014 small mammals of 14 species were examined, on which 1499 fleas of 13 species were collected. Most species of fleas are widely distributed throughout the territory. Ceratophyllus penicilliger, C. advenarius, Catallagia dacencoi, C. ioffi are mass species, Ceratophyllus calcarifer, C. indages, Leptopsylla ostsibirica, Doratopsylla birulai, Rhadinopsylla pseudodahurica are common species. Ceratophyllus taiganus, Amphipsylla marikovskii, Neopsylla acanthina and Tarsopsylla octodecimdentata are rather rare.", "contents": "[Fleas of small mammals in northern Amur Province and their distribution by biotopes (Aphaniptera)]. In June-September of 1975 on the southern slope of the Stanovoy ridge, in the region of construction of the Baikal-Amur main line 2014 small mammals of 14 species were examined, on which 1499 fleas of 13 species were collected. Most species of fleas are widely distributed throughout the territory. Ceratophyllus penicilliger, C. advenarius, Catallagia dacencoi, C. ioffi are mass species, Ceratophyllus calcarifer, C. indages, Leptopsylla ostsibirica, Doratopsylla birulai, Rhadinopsylla pseudodahurica are common species. Ceratophyllus taiganus, Amphipsylla marikovskii, Neopsylla acanthina and Tarsopsylla octodecimdentata are rather rare."}
{"id": "PMID:481906", "title": "Lysosomes and tegument pathology in the chemotherapy of schistosomiasis with 1,7-bis(p-aminophenoxy)heptane (153C51).", "content": "One therapeutic oral dose (400 mg/kg) of 153C51 administered to infested mice caused pathological changes in the dorsal region of the tegument of male Schistosoma mansoni during the period 3--24 h after treatment. These changes occurred prior to the 'hepatic shift'. At the ultrastructural level they consisted of a gradual accumulation in the tegument epidermis of numerous membranous inclusions with the characteristics of residual lysosomes and changes in the localization of acid phosphatase, a lysosomal enzyme. It seemed likely that these changes were due to inhibition or exhaustion of enzyme in the epidermis, followed by re-synthesis of enzyme in the cell bodies and its export to the epidermis. The elimination of hydrolytic activity from lysosomes in the epidermis would explain the accumulation of residual lysosomes. Drug-treated parasites retained their disguise of host red blood cell ghost antigens as shown by indirect fluorescent antibody-labelling and, therefore, it seemed unlikely that immunological factors could be important in producing the tegument pathology.", "contents": "Lysosomes and tegument pathology in the chemotherapy of schistosomiasis with 1,7-bis(p-aminophenoxy)heptane (153C51). One therapeutic oral dose (400 mg/kg) of 153C51 administered to infested mice caused pathological changes in the dorsal region of the tegument of male Schistosoma mansoni during the period 3--24 h after treatment. These changes occurred prior to the 'hepatic shift'. At the ultrastructural level they consisted of a gradual accumulation in the tegument epidermis of numerous membranous inclusions with the characteristics of residual lysosomes and changes in the localization of acid phosphatase, a lysosomal enzyme. It seemed likely that these changes were due to inhibition or exhaustion of enzyme in the epidermis, followed by re-synthesis of enzyme in the cell bodies and its export to the epidermis. The elimination of hydrolytic activity from lysosomes in the epidermis would explain the accumulation of residual lysosomes. Drug-treated parasites retained their disguise of host red blood cell ghost antigens as shown by indirect fluorescent antibody-labelling and, therefore, it seemed unlikely that immunological factors could be important in producing the tegument pathology."}
{"id": "PMID:481901", "title": "[Gamasid ticks of small mammals in the Charskaya Basin (Gamasoidea)].", "content": "Zooparasitological investigation of small mammals (17 species) was carried out in one of the regions of construction of the Baikal-Amur main line, Charskaya hollow. 8475 gamasid mites belonging to 8 families and represented by 25 species were collected. In addition, in the nests of the northern redbacked vole and root vole there were found gamasid mites belonging to 6 families. The mass species, Haemogamasus ambulans Thorell and Hirstionyssus isabellinus Oud., were recorded from all landscape zones, of the territory under investigation.", "contents": "[Gamasid ticks of small mammals in the Charskaya Basin (Gamasoidea)]. Zooparasitological investigation of small mammals (17 species) was carried out in one of the regions of construction of the Baikal-Amur main line, Charskaya hollow. 8475 gamasid mites belonging to 8 families and represented by 25 species were collected. In addition, in the nests of the northern redbacked vole and root vole there were found gamasid mites belonging to 6 families. The mass species, Haemogamasus ambulans Thorell and Hirstionyssus isabellinus Oud., were recorded from all landscape zones, of the territory under investigation."}
{"id": "PMID:481907", "title": "Synthesis of macromolecules by the epithelial surfaces of Schistosoma mansoni: an autoradiographic study.", "content": "The use of tritiated leucine as a marker for protein synthesis and of tritiated glucosamine as a marker for polysaccharide/glycoprotein synthesis, is described. Adult worms were pulse-labelled by incubation in medium containing the substrate. Labelled worms were then incubated in chase medium, without labelled substrate, for varying lengths of time before fixation. The distribution of label which had been incorporated into macromolecules in the worm tissues, was examined by light and electron microscope autoradiography. It was estimated that the tegument and tegument cell bodies were the source of 67--80%, and the gut epithelium of 20--30%, of exportable leucine-containing protein. Conversely, the gut epithelium was the source of 72%, and the tegument cells 28%, of exportable glucosamine-containing polysaccharide. The specific activity of labelled protein reached a peak in the tegument cytoplasm after 1.5 h of chase incubation. Half of the labelled protein was secreted into the worm's environment by 3 h of chase incubation. The half-life of secretory protein in gut cells appears to be around 2 h. Labelled protein disappears from the gut lumen relatively rapidly but labelled polysaccharide remains in the lumen at high specific activity for at least 24 h. The major carbohydrate labelled may be the glycocalyx on the luminal surface of the gut epithelial cells. The results suggest that the bulk of worm secretions have a rapid turnover with a half-life of a few hours. Against this background of rapid mass secretion a slower process of membrane turnover would be difficult to detect and quantitatively small.", "contents": "Synthesis of macromolecules by the epithelial surfaces of Schistosoma mansoni: an autoradiographic study. The use of tritiated leucine as a marker for protein synthesis and of tritiated glucosamine as a marker for polysaccharide/glycoprotein synthesis, is described. Adult worms were pulse-labelled by incubation in medium containing the substrate. Labelled worms were then incubated in chase medium, without labelled substrate, for varying lengths of time before fixation. The distribution of label which had been incorporated into macromolecules in the worm tissues, was examined by light and electron microscope autoradiography. It was estimated that the tegument and tegument cell bodies were the source of 67--80%, and the gut epithelium of 20--30%, of exportable leucine-containing protein. Conversely, the gut epithelium was the source of 72%, and the tegument cells 28%, of exportable glucosamine-containing polysaccharide. The specific activity of labelled protein reached a peak in the tegument cytoplasm after 1.5 h of chase incubation. Half of the labelled protein was secreted into the worm's environment by 3 h of chase incubation. The half-life of secretory protein in gut cells appears to be around 2 h. Labelled protein disappears from the gut lumen relatively rapidly but labelled polysaccharide remains in the lumen at high specific activity for at least 24 h. The major carbohydrate labelled may be the glycocalyx on the luminal surface of the gut epithelial cells. The results suggest that the bulk of worm secretions have a rapid turnover with a half-life of a few hours. Against this background of rapid mass secretion a slower process of membrane turnover would be difficult to detect and quantitatively small."}
{"id": "PMID:481908", "title": "Different levels of immunity to Schistosoma mansoni in the mouse: the role of variant cercariae.", "content": "Very variable levels of immunity to a second infection with Schistosoma mansoni were recorded in 7 strains of mice, 12--15 weeks following a small primary infection. When 2 or more strains of mice were assayed at the same time, less variation occurred within the experiment than between different experiments. This evidence suggested variation between pools of cercariae as the main cause of variability in immunity. In direct experiments in one strain of mouse, 2 different pools of cercariae stimulated widely different levels of immunity to the same challenge. Conversely, challenge infections drawn from different pools showed different susceptibility to immunity stimulated by the same primary infection. Individual clones of cercariae, from snails infected with single miracidia, showed a high level of susceptibility to immunity stimulated by a small bisexual infection, or were not susceptible at all. Antigenic polymorphism is the most likely explanation for the differences observed between clones of cercariae. However, indirect immunofluorescence showed the presence of at least 1 common antigen on the surface of schistosomula derived from different clones of cercariae and clone-specific antigens have not been detected.", "contents": "Different levels of immunity to Schistosoma mansoni in the mouse: the role of variant cercariae. Very variable levels of immunity to a second infection with Schistosoma mansoni were recorded in 7 strains of mice, 12--15 weeks following a small primary infection. When 2 or more strains of mice were assayed at the same time, less variation occurred within the experiment than between different experiments. This evidence suggested variation between pools of cercariae as the main cause of variability in immunity. In direct experiments in one strain of mouse, 2 different pools of cercariae stimulated widely different levels of immunity to the same challenge. Conversely, challenge infections drawn from different pools showed different susceptibility to immunity stimulated by the same primary infection. Individual clones of cercariae, from snails infected with single miracidia, showed a high level of susceptibility to immunity stimulated by a small bisexual infection, or were not susceptible at all. Antigenic polymorphism is the most likely explanation for the differences observed between clones of cercariae. However, indirect immunofluorescence showed the presence of at least 1 common antigen on the surface of schistosomula derived from different clones of cercariae and clone-specific antigens have not been detected."}
{"id": "PMID:481899", "title": "[Structural characteristics of the cuticule of the phytonematode, Aporcelaimellus obtusicaudatus in mechanical injury].", "content": "The fine structure of the cuticle of A. obtusicaudatus (Dorylaimidae) during the turgor unbalance was studied. Mechanical injury causes the delamination of the cuticle and reveals the tonofibrillary interrelations between its structures. The cortex and basal layer were found to possess the highest tensile strength. The peculiarities of the structures destruction dynamics depending on the removal from the perforation place were recognized.", "contents": "[Structural characteristics of the cuticule of the phytonematode, Aporcelaimellus obtusicaudatus in mechanical injury]. The fine structure of the cuticle of A. obtusicaudatus (Dorylaimidae) during the turgor unbalance was studied. Mechanical injury causes the delamination of the cuticle and reveals the tonofibrillary interrelations between its structures. The cortex and basal layer were found to possess the highest tensile strength. The peculiarities of the structures destruction dynamics depending on the removal from the perforation place were recognized."}
{"id": "PMID:481909", "title": "The effect of the expulsion phase of Trichinella spiralis on Hymenolepis diminuta infection in rats.", "content": "The effect of the intestinal changes brought about by the expulsion of Trichinella spiralis in rats was studied in relation to the growth and survival of a concurrent infection with Hymenolepis diminuta, a cestode not normally rejected by the rat in low-level infections. Growth of H. diminuta was stunted in rats given T. spiralis just before, or after, infection with H. diminuta, the stunting being more pronounced when the cestode was given closer to the period of inflammation. There was no loss of the cestode from dual-infected rats and no evidence for destrobilation was found. Lower T. spiralis burdens had a correspondingly weaker effect on growth of H. diminuta, and stunting was abolished by administration of the anti-inflammatory drug cortisone acetate. It is concluded that the stunting of H. diminuta is probably due to the non-specific inflammatory component of the rat's response to T. spiralis infection.", "contents": "The effect of the expulsion phase of Trichinella spiralis on Hymenolepis diminuta infection in rats. The effect of the intestinal changes brought about by the expulsion of Trichinella spiralis in rats was studied in relation to the growth and survival of a concurrent infection with Hymenolepis diminuta, a cestode not normally rejected by the rat in low-level infections. Growth of H. diminuta was stunted in rats given T. spiralis just before, or after, infection with H. diminuta, the stunting being more pronounced when the cestode was given closer to the period of inflammation. There was no loss of the cestode from dual-infected rats and no evidence for destrobilation was found. Lower T. spiralis burdens had a correspondingly weaker effect on growth of H. diminuta, and stunting was abolished by administration of the anti-inflammatory drug cortisone acetate. It is concluded that the stunting of H. diminuta is probably due to the non-specific inflammatory component of the rat's response to T. spiralis infection."}
{"id": "PMID:481910", "title": "Experimental infections with Cooperia oncophora (Railliet, 1918) in calves. Results of single infections with two graded dose levels of larvae.", "content": "Two experiments were carried out in which calves reared parasite-free were infected with a single dose of 3rd-stage larvae of Cooperia oncophora. In the first experiment the calves received 20 000 or 200 000 infective larvae and they were autopsied 28 or 56 days after the infection. In the second experiment the doses were the same but the animals were killed 14, 84 or 140 days after infection. If a dose of 20 000 was given, clinical signs were never observed, while at a dose level of 200 000 the weight gain was less on 56 and 84 days after the infection compared with the low-infected groups or the control animals. After 170 days the differences in weight gain were compensated. Faecal egg output was higher in the 200 000 groups only in the first period of patency, thereafter the calves in the 20 000 groups produced more. No obvious differences between the two infection levels were observed with regard to the haematological data. In the low-infected groups worm counts were only slightly lower when the results after 28 days were compared with those after 56 days. Also, the worm numbers after 14 days were almost equal to those after 84 days, while at 140 days 1 animal still had the same number, the other one had lost its worm burden. In the high-infected groups the worm loss was much quicker. After 28 days a great part of the population had already been lost. Obviously, at the 200 000 level the reaction of the host against the parasite was much stronger. Adult worms were expelled at a higher rate than early 4th-larval stages. In the first experiment worm measurements revealed differences between the length of females, males and spicules of males, these being significantly longer in the low-infected groups. Analysis of the distribution of worms over the small intestine showed that in the low-infected groups worms were mainly restricted to the first 6 metres. In the high-infected groups the worm population was distributed more evenly over the whole small intestine.", "contents": "Experimental infections with Cooperia oncophora (Railliet, 1918) in calves. Results of single infections with two graded dose levels of larvae. Two experiments were carried out in which calves reared parasite-free were infected with a single dose of 3rd-stage larvae of Cooperia oncophora. In the first experiment the calves received 20 000 or 200 000 infective larvae and they were autopsied 28 or 56 days after the infection. In the second experiment the doses were the same but the animals were killed 14, 84 or 140 days after infection. If a dose of 20 000 was given, clinical signs were never observed, while at a dose level of 200 000 the weight gain was less on 56 and 84 days after the infection compared with the low-infected groups or the control animals. After 170 days the differences in weight gain were compensated. Faecal egg output was higher in the 200 000 groups only in the first period of patency, thereafter the calves in the 20 000 groups produced more. No obvious differences between the two infection levels were observed with regard to the haematological data. In the low-infected groups worm counts were only slightly lower when the results after 28 days were compared with those after 56 days. Also, the worm numbers after 14 days were almost equal to those after 84 days, while at 140 days 1 animal still had the same number, the other one had lost its worm burden. In the high-infected groups the worm loss was much quicker. After 28 days a great part of the population had already been lost. Obviously, at the 200 000 level the reaction of the host against the parasite was much stronger. Adult worms were expelled at a higher rate than early 4th-larval stages. In the first experiment worm measurements revealed differences between the length of females, males and spicules of males, these being significantly longer in the low-infected groups. Analysis of the distribution of worms over the small intestine showed that in the low-infected groups worms were mainly restricted to the first 6 metres. In the high-infected groups the worm population was distributed more evenly over the whole small intestine."}
{"id": "PMID:481911", "title": "The uptake in vitro of dyes, monosaccharides and amino acids by the filarial worm Brugia pahangi.", "content": "The uptake in vitro of various substances by Brugia pahangi was investigated using infective larvae obtained from Aedes aegypti and worms removed from Meriones unguiculatus at 2, 3, 10, 20 and 90 days post-infection. Worms incubated in growth medium 199 containing 1% Trypan blue possessed demonstrable dye in the oral orifice, the anterior oesophageal lumen and the external openings of the vulva and the cloaca or anus but the dye was not found in the gut lumen even after incubation for 24 h. No uptake of ferritin particles into the intestine of the worms was found and no fluorescence could be demonstrated in the gut lumen of worms incubated in medium containing 50% (v/v) fluorescein isothiocyanate-conjugated calf serum for up to 24 h. Trypan blue uptake by the gut of Aspiculuris tetraptera was clearly observed after incubation for several hours. The uptake of D-glucose and L-leucine by B. pahangi was demonstrated using autoradiographic and scintillation counting techniques and incorporation into worm tissues was detected. Glucose was found to be readily incorporated in the apical, glycogen-rich areas of the myocytes of worms of all ages studied and in the uterine epithelium of the adult female. In contrast, a lower incorporation of D-glucose was found in the eggs, embryos and vas deferens and especially in the gut. The incorporation of L-leucine occurred throughout the tissue of the worms during a 30 min incubation. Labelling was also located over the surface of the cuticle of the worms, when incubated for a period of 15 to 60 min in L-[H]leucine. Scintillation counting techniques demonstrated that there was no uptake of 14C-labelled L-glucose or sucrose by B. pahangi. The data presented on the uptake in vitro of nutrients or other compounds by infective larvae and adult stages of B. pahangi did not demonstrate an intestinal route of uptake but indicated that the transcuticular route of uptake may be employed.", "contents": "The uptake in vitro of dyes, monosaccharides and amino acids by the filarial worm Brugia pahangi. The uptake in vitro of various substances by Brugia pahangi was investigated using infective larvae obtained from Aedes aegypti and worms removed from Meriones unguiculatus at 2, 3, 10, 20 and 90 days post-infection. Worms incubated in growth medium 199 containing 1% Trypan blue possessed demonstrable dye in the oral orifice, the anterior oesophageal lumen and the external openings of the vulva and the cloaca or anus but the dye was not found in the gut lumen even after incubation for 24 h. No uptake of ferritin particles into the intestine of the worms was found and no fluorescence could be demonstrated in the gut lumen of worms incubated in medium containing 50% (v/v) fluorescein isothiocyanate-conjugated calf serum for up to 24 h. Trypan blue uptake by the gut of Aspiculuris tetraptera was clearly observed after incubation for several hours. The uptake of D-glucose and L-leucine by B. pahangi was demonstrated using autoradiographic and scintillation counting techniques and incorporation into worm tissues was detected. Glucose was found to be readily incorporated in the apical, glycogen-rich areas of the myocytes of worms of all ages studied and in the uterine epithelium of the adult female. In contrast, a lower incorporation of D-glucose was found in the eggs, embryos and vas deferens and especially in the gut. The incorporation of L-leucine occurred throughout the tissue of the worms during a 30 min incubation. Labelling was also located over the surface of the cuticle of the worms, when incubated for a period of 15 to 60 min in L-[H]leucine. Scintillation counting techniques demonstrated that there was no uptake of 14C-labelled L-glucose or sucrose by B. pahangi. The data presented on the uptake in vitro of nutrients or other compounds by infective larvae and adult stages of B. pahangi did not demonstrate an intestinal route of uptake but indicated that the transcuticular route of uptake may be employed."}
{"id": "PMID:481903", "title": "[Seasonal dynamics of the development and reproduction of parthenogenetic generations of Xiphidiocercaria sp. VII Odening 1962 (Trematoda: Plagiorchiidae) in the mollusk, Coretus corneus].", "content": "Intensity of reproduction of parthenogenetic generations of Xiphidiocercaria sp. VII Odening 1962 was shown to exhibit a seasonal dependence. Comparative studies of generative and somatic cell nuclei size, amount of divisional cells, quantity of cercariae and embryos were conducted in summer and winter.", "contents": "[Seasonal dynamics of the development and reproduction of parthenogenetic generations of Xiphidiocercaria sp. VII Odening 1962 (Trematoda: Plagiorchiidae) in the mollusk, Coretus corneus]. Intensity of reproduction of parthenogenetic generations of Xiphidiocercaria sp. VII Odening 1962 was shown to exhibit a seasonal dependence. Comparative studies of generative and somatic cell nuclei size, amount of divisional cells, quantity of cercariae and embryos were conducted in summer and winter."}
{"id": "PMID:481912", "title": "In vitro variation of glycogen content in three sheep nematodes.", "content": "In vitro variation of glycogen content under aerobic conditions was measured on fresh weight basis in 3 sheep nematodes inhabiting different niches; Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis. The parasites were saparated into species and then sexes and starved for varying periods of time up to 24 h in glucose-free physiological saline. The differences between females and males and among the species with respect to glycogen content and its rate of change with time are discussed.", "contents": "In vitro variation of glycogen content in three sheep nematodes. In vitro variation of glycogen content under aerobic conditions was measured on fresh weight basis in 3 sheep nematodes inhabiting different niches; Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis. The parasites were saparated into species and then sexes and starved for varying periods of time up to 24 h in glucose-free physiological saline. The differences between females and males and among the species with respect to glycogen content and its rate of change with time are discussed."}
{"id": "PMID:481941", "title": "Serum factor in cystic fibrosis: correlation with clinical parameters.", "content": "The relationship between the activity of the cystic fibrosis serum ciliary dyskinesia factor, detected by the rabbit tracheal bioassay, and clinical status of the patient has been investigated in children, 1-24 years old, with cystic fibrosis. No significant correlation was found between the amount of serum factor activity and age, clinical status assessed by the Schwachman score, pulmonary function (vital capacity, functional residual capacity, total lung capacity, residual volume, maximum breathing capacity, maximal midinspiratory flow, and maximal midexpiratory flow) or blood gas levels (pCO2, pO2). The activity showed no significant relationship to serum galactosyltransferase activity in children with cystic fibrosis.", "contents": "Serum factor in cystic fibrosis: correlation with clinical parameters. The relationship between the activity of the cystic fibrosis serum ciliary dyskinesia factor, detected by the rabbit tracheal bioassay, and clinical status of the patient has been investigated in children, 1-24 years old, with cystic fibrosis. No significant correlation was found between the amount of serum factor activity and age, clinical status assessed by the Schwachman score, pulmonary function (vital capacity, functional residual capacity, total lung capacity, residual volume, maximum breathing capacity, maximal midinspiratory flow, and maximal midexpiratory flow) or blood gas levels (pCO2, pO2). The activity showed no significant relationship to serum galactosyltransferase activity in children with cystic fibrosis."}
{"id": "PMID:481942", "title": "Plasma renin activity related to sodium balance, renal function and urinary vasopressin in the newborn infant.", "content": "Plasma renin activity was determined in 25 healthy, full-term, newborn infants aged 1 day to 9 weeks. High values were found, the mean level at 1-2 days of life (24.8 +/- 8.4 ng/ml/hr, SE) being significantly higher than the mean levels at 7-9 days (5.8 +/- 1.5) and at 4-9 weeks (8.1 +/- 1.3) (P less than 0.05). No correlation was found between plasma renin activity and systolic blood pressure, hematocrit, creatinine clearance, serum sodium, or serum potassium. Plasma renin activity (log values) was inversely correlated with sodium intake (r = -0.58) or with urinary sodium (r = -0.44), and positively with urinary osmolality (r = 0.67). The correlations reached higher coefficients if only infants aged less than or equal to 9 days were considered. In addition, vasopressin was measured by radioimmunoassay in the urine. The daily excretion was lower in newborn infants (9.4 +/- 1.6 ng/m2/day, SE, at 1-2 days of postnatal life) than in healthy children (37.1 +/- 5.6), and was significantly correlated with creatinine clearance (r = 0.69), but not with urinary osmolality.", "contents": "Plasma renin activity related to sodium balance, renal function and urinary vasopressin in the newborn infant. Plasma renin activity was determined in 25 healthy, full-term, newborn infants aged 1 day to 9 weeks. High values were found, the mean level at 1-2 days of life (24.8 +/- 8.4 ng/ml/hr, SE) being significantly higher than the mean levels at 7-9 days (5.8 +/- 1.5) and at 4-9 weeks (8.1 +/- 1.3) (P less than 0.05). No correlation was found between plasma renin activity and systolic blood pressure, hematocrit, creatinine clearance, serum sodium, or serum potassium. Plasma renin activity (log values) was inversely correlated with sodium intake (r = -0.58) or with urinary sodium (r = -0.44), and positively with urinary osmolality (r = 0.67). The correlations reached higher coefficients if only infants aged less than or equal to 9 days were considered. In addition, vasopressin was measured by radioimmunoassay in the urine. The daily excretion was lower in newborn infants (9.4 +/- 1.6 ng/m2/day, SE, at 1-2 days of postnatal life) than in healthy children (37.1 +/- 5.6), and was significantly correlated with creatinine clearance (r = 0.69), but not with urinary osmolality."}
{"id": "PMID:481943", "title": "Human propionyl CoA carboxylase: some properties of the partially purified enzyme in fibroblasts from controls and patients with propionic acidemia.", "content": "We report some properties of propionyl CoA carboxylase (PCC) partially purified from cultured human fibroblasts obtained from controls and several patients with propionic acidemia. A series of steps (Triton X-100 treatment, high speed centrifugation, ammonium sulfate precipitation, and density gradient centrifugation) led to 100- to 300-fold purification of control enzyme. Control PCC had a molecular weight of nearly 700,000, contained biotin, demonstrated a pH optinum at 8.0-8.5, was activated by potassium, and followed Michaelis-Menten kinetics for each of its substrates. It was distinguished from acetyl CoA carboxylase immunologically as well as by differential purification. Each of seven lines from patients with propionic acidemia had clearly detectable PCC activity which was less than 5% of that in control lines. Although yields were poor and purification less extensive than in control lines, mutant PCC was enriched 2- to 40-fold by the same procedures employed for the control enzyme. Mutant enzyme had a pH optimum, ionic requirements, and substrate Km's similar to those of control PCC, but was distinctly more labile to both cold and heat. These findings suggest that the markedly reduced activity of PCC in these patients results from a mutation in the PCC structural gene locus or loci which leads to the synthesis of altered enzyme protein molecules.", "contents": "Human propionyl CoA carboxylase: some properties of the partially purified enzyme in fibroblasts from controls and patients with propionic acidemia. We report some properties of propionyl CoA carboxylase (PCC) partially purified from cultured human fibroblasts obtained from controls and several patients with propionic acidemia. A series of steps (Triton X-100 treatment, high speed centrifugation, ammonium sulfate precipitation, and density gradient centrifugation) led to 100- to 300-fold purification of control enzyme. Control PCC had a molecular weight of nearly 700,000, contained biotin, demonstrated a pH optinum at 8.0-8.5, was activated by potassium, and followed Michaelis-Menten kinetics for each of its substrates. It was distinguished from acetyl CoA carboxylase immunologically as well as by differential purification. Each of seven lines from patients with propionic acidemia had clearly detectable PCC activity which was less than 5% of that in control lines. Although yields were poor and purification less extensive than in control lines, mutant PCC was enriched 2- to 40-fold by the same procedures employed for the control enzyme. Mutant enzyme had a pH optimum, ionic requirements, and substrate Km's similar to those of control PCC, but was distinctly more labile to both cold and heat. These findings suggest that the markedly reduced activity of PCC in these patients results from a mutation in the PCC structural gene locus or loci which leads to the synthesis of altered enzyme protein molecules."}
{"id": "PMID:481945", "title": "Altered intracellular calcium in fibroblasts from patients with cystic fibrosis and heterozygotes.", "content": "The importance of intracellular calcium (Ca) in secretion and transmembrane ion movement led us to study Ca in cells from patients with cystic fibrosis (CF) which is a lethal genetic exocrinopathy. Skin fibroblasts from patients with CF, obligate heterozygotes (HZ), and age- and sex-matched controls (C) were used in matched pair experiments measuring 45Ca exchange into and efflux from the cells over time. CF cell lines and HZ cell lines exhibit increased 45Ca exchange when compared with their respective controls (P less than 0.005). The magnitude of this difference (approximately 30%) is not reduced when cells are washed with lanthanum chloride after the exchange period. This difference is likely attributable to an altered capacity of one or more of the intracellular Ca sequestering organelles. Further evidence for this explanation was seen in 45Ca efflux experiments in which CF cells retained a higher percent of their initial 0-time 45Ca than did C cells late in the efflux period (P less than 0.05). The finding of an altered Ca pool size in both CF and particularly HZ cells suggests that altered Ca metabolism is related to the basic gene defect in CF.", "contents": "Altered intracellular calcium in fibroblasts from patients with cystic fibrosis and heterozygotes. The importance of intracellular calcium (Ca) in secretion and transmembrane ion movement led us to study Ca in cells from patients with cystic fibrosis (CF) which is a lethal genetic exocrinopathy. Skin fibroblasts from patients with CF, obligate heterozygotes (HZ), and age- and sex-matched controls (C) were used in matched pair experiments measuring 45Ca exchange into and efflux from the cells over time. CF cell lines and HZ cell lines exhibit increased 45Ca exchange when compared with their respective controls (P less than 0.005). The magnitude of this difference (approximately 30%) is not reduced when cells are washed with lanthanum chloride after the exchange period. This difference is likely attributable to an altered capacity of one or more of the intracellular Ca sequestering organelles. Further evidence for this explanation was seen in 45Ca efflux experiments in which CF cells retained a higher percent of their initial 0-time 45Ca than did C cells late in the efflux period (P less than 0.05). The finding of an altered Ca pool size in both CF and particularly HZ cells suggests that altered Ca metabolism is related to the basic gene defect in CF."}
{"id": "PMID:481946", "title": "Utilization of liposomes for correction of the metabolic and bactericidal deficiencies in chronic granulomatous disease.", "content": "IgG-coated liposomes containing glucose oxidase (GO) in order to provide a means of generating H2O2 were prepared. Leukocytes from patients with chronic granulomatous disease (CGD) which are lacking a means of generating H2O2, ingested the IgG-coated liposomes and metabolic oxidative deficiencies of glucose-1-14C oxidation and iodination were normalized. Both of these activities have been shown to depend upon the availability of H2O2 within polymorphonuclear leukocytes. Improvement in the capacity to kill Staphylococcus aureus by chronic granulomatous disease leukocytes were observed under similar conditions. Thus, it is possible to restore the oxidative metabolic capacities to CGD leukocytes by the introduction of glucose oxidase containing liposomes to these cells.", "contents": "Utilization of liposomes for correction of the metabolic and bactericidal deficiencies in chronic granulomatous disease. IgG-coated liposomes containing glucose oxidase (GO) in order to provide a means of generating H2O2 were prepared. Leukocytes from patients with chronic granulomatous disease (CGD) which are lacking a means of generating H2O2, ingested the IgG-coated liposomes and metabolic oxidative deficiencies of glucose-1-14C oxidation and iodination were normalized. Both of these activities have been shown to depend upon the availability of H2O2 within polymorphonuclear leukocytes. Improvement in the capacity to kill Staphylococcus aureus by chronic granulomatous disease leukocytes were observed under similar conditions. Thus, it is possible to restore the oxidative metabolic capacities to CGD leukocytes by the introduction of glucose oxidase containing liposomes to these cells."}
{"id": "PMID:481948", "title": "Small intestinal mucosal fatty acid uptake and esterification in infants and children.", "content": "Oleic acid uptake and esterification in intact intestinal mucosa were studied in 14 infants and children with chronic non-specific diarrhea, but histologically normal small intestinal mucosal biopsies, using an in vitro technique. The uptake rate was 5.876 +/- 1.942 nmol fatty acid/mg Nigrogen/minute and the esterification rate was 4.060 +/- 1.010 nmol fatty acid/mg Nitrogen/minute, comparable to previous adult esterification studies. No effect of age on either esterification or uptake was present. Mucosal injury resulted in significant reductions in esterification (p less than 0.001) and uptake (p less than 0.05) compared to controls. Bile acid deficiencies led to reductions in mucosal esterification (p less than 0.05) but not uptake.", "contents": "Small intestinal mucosal fatty acid uptake and esterification in infants and children. Oleic acid uptake and esterification in intact intestinal mucosa were studied in 14 infants and children with chronic non-specific diarrhea, but histologically normal small intestinal mucosal biopsies, using an in vitro technique. The uptake rate was 5.876 +/- 1.942 nmol fatty acid/mg Nigrogen/minute and the esterification rate was 4.060 +/- 1.010 nmol fatty acid/mg Nitrogen/minute, comparable to previous adult esterification studies. No effect of age on either esterification or uptake was present. Mucosal injury resulted in significant reductions in esterification (p less than 0.001) and uptake (p less than 0.05) compared to controls. Bile acid deficiencies led to reductions in mucosal esterification (p less than 0.05) but not uptake."}
{"id": "PMID:481949", "title": "Bidirectional placental transfer of glucose and its turnover in fetal and maternal sheep.", "content": "Glucose biokinetics were assessed simultaneously in the pregnant ewe and its fetus by a primed constant infusion of 2-3H glucose and U-14C glucose. Late in gestation fetal glucose turnover was 27.3 +/- 3.7 mg/min; expressed in terms of fetal weight this is 6 to 10 mg/kg/min. In the fed state the results indicated that all of the fetal glucose turnover was derived from the mother via placental transfer and there was no evidence that the fetus was capable of glucose production. Maternal glucose turnover was 145.6 +/- 9.3 mg/min (2.8 mg/kg/min). There was a significant amount of glucose (16.3 +/- 2.3 mg/min) transferred from the fetus to the mother. This feto-maternal transfer of glucose accounted for 11% of the maternal glucose turnover and approximately 50% of the total glucose coming to the fetus from the mother. This study provides the first in vivo simultaneous quantification of the bidirectional glucose transfer across the placenta.", "contents": "Bidirectional placental transfer of glucose and its turnover in fetal and maternal sheep. Glucose biokinetics were assessed simultaneously in the pregnant ewe and its fetus by a primed constant infusion of 2-3H glucose and U-14C glucose. Late in gestation fetal glucose turnover was 27.3 +/- 3.7 mg/min; expressed in terms of fetal weight this is 6 to 10 mg/kg/min. In the fed state the results indicated that all of the fetal glucose turnover was derived from the mother via placental transfer and there was no evidence that the fetus was capable of glucose production. Maternal glucose turnover was 145.6 +/- 9.3 mg/min (2.8 mg/kg/min). There was a significant amount of glucose (16.3 +/- 2.3 mg/min) transferred from the fetus to the mother. This feto-maternal transfer of glucose accounted for 11% of the maternal glucose turnover and approximately 50% of the total glucose coming to the fetus from the mother. This study provides the first in vivo simultaneous quantification of the bidirectional glucose transfer across the placenta."}
{"id": "PMID:481950", "title": "Changes in lymphocyte sub-populations in the blood of healthy and sick newborn infants.", "content": "Normal values for the percentages of lymphocytes carrying surface membrane-bound immunoglobulin, or rosetting with sheep erythrocytes, ox erythrocytes coated with rabbit immunoglobulin G, or ox erythrocytes coated with rabbit immunoglobulin M and reacted with human serum partially de-complemented with zymozan, have been estimated for populations from human umbilical cord blood and the peripheral blood of babies aged one week and one month. These samples have been compared with values obtained from normal adult volunteers and eighteen newborn infants, measured during and after a suspected infective episode. The dynamic change in those lymphocytes from normal infants staining for both IgM and IgD is shown, demonstrating that the number of circulating cells of this typeapproaches adult levels by the age of one month. Falls in the levels of all three types of rosetting lymphocyte were demonstrated over the same period. A drop in the number of lymphocytes forming sheep erythrocyte rosettes, and particularly those binding C3b-coated ox erythrocytes, in those babies suspected of infection showed a correlation with acute septicaemia or viraemia.", "contents": "Changes in lymphocyte sub-populations in the blood of healthy and sick newborn infants. Normal values for the percentages of lymphocytes carrying surface membrane-bound immunoglobulin, or rosetting with sheep erythrocytes, ox erythrocytes coated with rabbit immunoglobulin G, or ox erythrocytes coated with rabbit immunoglobulin M and reacted with human serum partially de-complemented with zymozan, have been estimated for populations from human umbilical cord blood and the peripheral blood of babies aged one week and one month. These samples have been compared with values obtained from normal adult volunteers and eighteen newborn infants, measured during and after a suspected infective episode. The dynamic change in those lymphocytes from normal infants staining for both IgM and IgD is shown, demonstrating that the number of circulating cells of this typeapproaches adult levels by the age of one month. Falls in the levels of all three types of rosetting lymphocyte were demonstrated over the same period. A drop in the number of lymphocytes forming sheep erythrocyte rosettes, and particularly those binding C3b-coated ox erythrocytes, in those babies suspected of infection showed a correlation with acute septicaemia or viraemia."}
{"id": "PMID:481953", "title": "Postnatal development of renin-angiotensin-aldosterone system, RAAS, in relation to electrolyte balance in premature infants.", "content": "In an attempt to provide information about the role of RAAS in development of late hyponatremia in low-birthweight neonates, simultaneous measurement of plasma renin activity, (PRA), plasma aldosterone concentration (PA), and urinary aldosterone excretion (UAE) was made using RIA methods along with determination of Na and K balance weekly up to the 6th week of life. Seven healthy male infants with mean birthweight of 1580 g, range: 1160-1850 g, and mean gestational age of 31 weeks, range: 30-32 weeks, were selected for the study. Due to the increased urinary Na loss, negative Na balance developed in the first 2 weeks followed positive balance thereafter. PRA, PA, and UAE increased tremendously from the initially high values of 18.2 +/- 4.1 ng/ml/hr, 1.7 +/- 0.5 ng/ml, and 2.6 +/- 0.4 microgram/day, mean and SEM, to their maximum of 78.6 +/- 18.1 ng/ml/hr, p less than 0.01, 6.8 +/- 3.7 ng/ml, p less than 0.05, and 26.4 +/- 2.9 microgram/dayp less than 0.01, in the 3rd week, respectively. Later on, gradual declines occurred, however, PRA, PA, and UAE remained highly elevated even at the 6th week with values of 45.5 +/- 15 ng/ml/hr, 1.6 +/- 0.5 ng/ml, and 14.5 +/- 1.4 microgram/day, respectively. It is suggested that late hyponatremia of premature infants is due to tubular unresponsiveness to aldosterone and not to inadequate response of RAAS to stimulation.", "contents": "Postnatal development of renin-angiotensin-aldosterone system, RAAS, in relation to electrolyte balance in premature infants. In an attempt to provide information about the role of RAAS in development of late hyponatremia in low-birthweight neonates, simultaneous measurement of plasma renin activity, (PRA), plasma aldosterone concentration (PA), and urinary aldosterone excretion (UAE) was made using RIA methods along with determination of Na and K balance weekly up to the 6th week of life. Seven healthy male infants with mean birthweight of 1580 g, range: 1160-1850 g, and mean gestational age of 31 weeks, range: 30-32 weeks, were selected for the study. Due to the increased urinary Na loss, negative Na balance developed in the first 2 weeks followed positive balance thereafter. PRA, PA, and UAE increased tremendously from the initially high values of 18.2 +/- 4.1 ng/ml/hr, 1.7 +/- 0.5 ng/ml, and 2.6 +/- 0.4 microgram/day, mean and SEM, to their maximum of 78.6 +/- 18.1 ng/ml/hr, p less than 0.01, 6.8 +/- 3.7 ng/ml, p less than 0.05, and 26.4 +/- 2.9 microgram/dayp less than 0.01, in the 3rd week, respectively. Later on, gradual declines occurred, however, PRA, PA, and UAE remained highly elevated even at the 6th week with values of 45.5 +/- 15 ng/ml/hr, 1.6 +/- 0.5 ng/ml, and 14.5 +/- 1.4 microgram/day, respectively. It is suggested that late hyponatremia of premature infants is due to tubular unresponsiveness to aldosterone and not to inadequate response of RAAS to stimulation."}
{"id": "PMID:481954", "title": "In vivo evaluation of monopolar intravascular PO2 electrodes.", "content": "Ten intravascular electrodes were evaluated on ten rabbits. The mean stabilized time of 118 +/- 52 min was excessively long for clinical use. The in vitro cal factor was not reproducible nor accurate. The differences between the PO2 values measured by the electrode and those obtained from intermittent blood samples were within +/- 10 mm Hg during the first 12 hr using the in vivo calibration after electrode stabilization. After 12 hr, the electrode PO2 values using the in vitro cal factor or based upon the in vivo calibration were increased significantly indicating a possible membrane rupture. This occured more rapidly in the animal experiments than in the in vitro studies using a tonometer, suggesting that continuous flow and pressure changes inside of the aorta contribute greatly to mechanical breakage of the membrane. Histologic evaluation indicated the electrode catheter system was thrombogenic at the level of the electrode tip and this thrombogenicity was primarily related to intimal injury caused by the electrode tip movement as a result of flow and pressure changes inside the aorta.", "contents": "In vivo evaluation of monopolar intravascular PO2 electrodes. Ten intravascular electrodes were evaluated on ten rabbits. The mean stabilized time of 118 +/- 52 min was excessively long for clinical use. The in vitro cal factor was not reproducible nor accurate. The differences between the PO2 values measured by the electrode and those obtained from intermittent blood samples were within +/- 10 mm Hg during the first 12 hr using the in vivo calibration after electrode stabilization. After 12 hr, the electrode PO2 values using the in vitro cal factor or based upon the in vivo calibration were increased significantly indicating a possible membrane rupture. This occured more rapidly in the animal experiments than in the in vitro studies using a tonometer, suggesting that continuous flow and pressure changes inside of the aorta contribute greatly to mechanical breakage of the membrane. Histologic evaluation indicated the electrode catheter system was thrombogenic at the level of the electrode tip and this thrombogenicity was primarily related to intimal injury caused by the electrode tip movement as a result of flow and pressure changes inside the aorta."}
{"id": "PMID:481956", "title": "Increased heparin binding in cystic fibrosis: a reflection of altered glycoprotein biosynthesis?", "content": "Some of the serum proteins which bind to heparin and contribute to the pH 5.57 \"heparin binding capacity\" of human serum are glycoproteins; those from cystic fibrosis serum were found to be 27% higher in fucose (methylpentose) content, 27% lower in sialic acid content, and 31% lower in hexose content when compared to heparin-precipitated serum glycoproteins from normal control subjects. Hexosamine content of the heparin-precipitated serum glycoproteins was the same. Results of this preliminary investigation indicate that altered carbohydrate composition in serum glycoproteins may affect significantly their heparin binding capacity.", "contents": "Increased heparin binding in cystic fibrosis: a reflection of altered glycoprotein biosynthesis? Some of the serum proteins which bind to heparin and contribute to the pH 5.57 \"heparin binding capacity\" of human serum are glycoproteins; those from cystic fibrosis serum were found to be 27% higher in fucose (methylpentose) content, 27% lower in sialic acid content, and 31% lower in hexose content when compared to heparin-precipitated serum glycoproteins from normal control subjects. Hexosamine content of the heparin-precipitated serum glycoproteins was the same. Results of this preliminary investigation indicate that altered carbohydrate composition in serum glycoproteins may affect significantly their heparin binding capacity."}
{"id": "PMID:481958", "title": "Abnormal copper metabolism in Menke's steely-hair syndrome.", "content": "Copper (Cu) metabolism was selectively studied in seven infants with Menke's steely-hair syndrome (SHS). A daily oral regimen of CuSO4 (584 microgram Cu/kg) and L-histidine (100 mg/kg) in three infants produced an increase in serum Cu concentrations ranging from 33-95% of normal, but without the formation of ceruloplasmin. Cohn serum protein fractionation after oral Cu/L-histidine loading showed a disproportionate accumulation of Cu in the albumin fraction (V). The electron spin resonance spectrum of fraction V showed a heightened signal for the SHS patients, suggesting that an increased concentration of a radical Cu species is present after oral loading. The Sephadex G-150 chromatographic profile of serum fraction V in SHS did not differ significantly from controls. These results suggest that, in SHS, Cu absorbed in the presence of L-histidine is in an abnormal complex involving albumin, which does not allow for holoceruloplasmin biosynthesis. Cu and ceruloplasmin concentrations in the cord blood specimen of an infant who went on to develop SHS were normal, a finding which may account for the transient period of seemingly normal development after birth in SHS patients. An almost 6-fold difference in mean Cu concentration was observed in SHS fibroblasts compared to controls. Fibroblast Cu concentration was elevated in one to two possible maternal heterozygotes, a finding which may permit diagnosis of the carrier state for some SHS heterozygotes.", "contents": "Abnormal copper metabolism in Menke's steely-hair syndrome. Copper (Cu) metabolism was selectively studied in seven infants with Menke's steely-hair syndrome (SHS). A daily oral regimen of CuSO4 (584 microgram Cu/kg) and L-histidine (100 mg/kg) in three infants produced an increase in serum Cu concentrations ranging from 33-95% of normal, but without the formation of ceruloplasmin. Cohn serum protein fractionation after oral Cu/L-histidine loading showed a disproportionate accumulation of Cu in the albumin fraction (V). The electron spin resonance spectrum of fraction V showed a heightened signal for the SHS patients, suggesting that an increased concentration of a radical Cu species is present after oral loading. The Sephadex G-150 chromatographic profile of serum fraction V in SHS did not differ significantly from controls. These results suggest that, in SHS, Cu absorbed in the presence of L-histidine is in an abnormal complex involving albumin, which does not allow for holoceruloplasmin biosynthesis. Cu and ceruloplasmin concentrations in the cord blood specimen of an infant who went on to develop SHS were normal, a finding which may account for the transient period of seemingly normal development after birth in SHS patients. An almost 6-fold difference in mean Cu concentration was observed in SHS fibroblasts compared to controls. Fibroblast Cu concentration was elevated in one to two possible maternal heterozygotes, a finding which may permit diagnosis of the carrier state for some SHS heterozygotes."}
{"id": "PMID:481959", "title": "The effect of hemoglobin F-Chesapeake (alpha 2 92 Arg. leads to Leu gamma 2) on fetal oxygen affinity and erythropoiesis.", "content": "A carrier of hemoglobin Chesapeake, born of a normal mother, had a cord-blood hematocrit of 60%. The oxygen affinity of his blood was increased. Hemoglobin F-Chesapeake (alpha 2 Ches gamma 2), partially purified from the infant's blood, had oxygen affinity greater than that of hemoglobin A, but less than that of the adult form of the abnormal hemoglobin (alpha 2 Ches beta 2). These findings suggest that the conformation of that part of the gamma chain which contacts the site of amino acid substitution in Hb F-Chesapeake is similar to the analogous region of hemoglobin F. They also support the hypothesis that regulation of erythropoiesis in late fetal life is similar to that of the adult, and is under fetal control.", "contents": "The effect of hemoglobin F-Chesapeake (alpha 2 92 Arg. leads to Leu gamma 2) on fetal oxygen affinity and erythropoiesis. A carrier of hemoglobin Chesapeake, born of a normal mother, had a cord-blood hematocrit of 60%. The oxygen affinity of his blood was increased. Hemoglobin F-Chesapeake (alpha 2 Ches gamma 2), partially purified from the infant's blood, had oxygen affinity greater than that of hemoglobin A, but less than that of the adult form of the abnormal hemoglobin (alpha 2 Ches beta 2). These findings suggest that the conformation of that part of the gamma chain which contacts the site of amino acid substitution in Hb F-Chesapeake is similar to the analogous region of hemoglobin F. They also support the hypothesis that regulation of erythropoiesis in late fetal life is similar to that of the adult, and is under fetal control."}
{"id": "PMID:481960", "title": "Developmental aspects of renal beta-amino acid transport I. Ontogeny of taurine reabsorption and accumulation in rat renal cortex.", "content": "Newborn Sprague-Dawley rat pups were found to have reduced net tubular reclamation of the beta-amino acid taurine in vivo. The reabsorption of this compound increased between the 2nd and 4th week of life and the excreted taurine fell despite a rise in glomerular filtration rate indicative of increased transport with maturation. Kidney cortex in vivo accumulated taurine from plasma against a large chemical gradient. Newborn cortex slices in vitro accumulated taurine to higher levels at steady state at less than 0.4 mM, but uptake was less efficient at higher taurine concentrations. Further metabolism of taurine by cortex slices was not found at any age. Accumulation in adult and newborn cortex was greatest at 10-15 mEa/liter K in the external medium. Slices from younger animals had slightly higher pH optima for uptake and temperature elevation increases uptake more in neonatal than in adult kidney.", "contents": "Developmental aspects of renal beta-amino acid transport I. Ontogeny of taurine reabsorption and accumulation in rat renal cortex. Newborn Sprague-Dawley rat pups were found to have reduced net tubular reclamation of the beta-amino acid taurine in vivo. The reabsorption of this compound increased between the 2nd and 4th week of life and the excreted taurine fell despite a rise in glomerular filtration rate indicative of increased transport with maturation. Kidney cortex in vivo accumulated taurine from plasma against a large chemical gradient. Newborn cortex slices in vitro accumulated taurine to higher levels at steady state at less than 0.4 mM, but uptake was less efficient at higher taurine concentrations. Further metabolism of taurine by cortex slices was not found at any age. Accumulation in adult and newborn cortex was greatest at 10-15 mEa/liter K in the external medium. Slices from younger animals had slightly higher pH optima for uptake and temperature elevation increases uptake more in neonatal than in adult kidney."}
{"id": "PMID:481962", "title": "Immediate and late ventillatory response to high and low O2 in preterm infants and adult subjects.", "content": "The differences in the immediate (30 sec or 1 min) and late (5 min) ventilatory response to high and low O2 have not been quantitated in preterm infants and adult subjects using the same methods. It was thought that these differences might explain the paradoxical ventilatory response to CO2 at various O2 concentrations in preterm infants (12). Thus, 9 preterm infants and 10 adult subjects were given 21% O2 to breathe and then 100 or 15% O2 for 5 min each. Adults also breathed 15% O2 before 100% O2 or 12% O2 in order to make their resting arterial PO2 more comparable to those of infants breathing 21% O2. The ventilatory response to 100% O2 was the same in preterm infants and adult subjects, but the late response to 15% O2 remained paradoxical, ventilation decreasing at 5 min by 18% in infants and increasing by 19% in adults. The authors conclude: 1) the traditional concept of the ventilatory response to 100% O2 being different in infants and adult subjects is false; 2) the notion that the response to low O2 is paradoxical in infants is correct; and 3) the data do not explain why the response to CO2 under various background concentrations of O2 in infants is the reverse of that in adult subjects, but the depressed ventilatory response to hypoxia in infants may justify, at least in part, their flatter response to CO2 during low O2 breathing.", "contents": "Immediate and late ventillatory response to high and low O2 in preterm infants and adult subjects. The differences in the immediate (30 sec or 1 min) and late (5 min) ventilatory response to high and low O2 have not been quantitated in preterm infants and adult subjects using the same methods. It was thought that these differences might explain the paradoxical ventilatory response to CO2 at various O2 concentrations in preterm infants (12). Thus, 9 preterm infants and 10 adult subjects were given 21% O2 to breathe and then 100 or 15% O2 for 5 min each. Adults also breathed 15% O2 before 100% O2 or 12% O2 in order to make their resting arterial PO2 more comparable to those of infants breathing 21% O2. The ventilatory response to 100% O2 was the same in preterm infants and adult subjects, but the late response to 15% O2 remained paradoxical, ventilation decreasing at 5 min by 18% in infants and increasing by 19% in adults. The authors conclude: 1) the traditional concept of the ventilatory response to 100% O2 being different in infants and adult subjects is false; 2) the notion that the response to low O2 is paradoxical in infants is correct; and 3) the data do not explain why the response to CO2 under various background concentrations of O2 in infants is the reverse of that in adult subjects, but the depressed ventilatory response to hypoxia in infants may justify, at least in part, their flatter response to CO2 during low O2 breathing."}
{"id": "PMID:481963", "title": "Inhibition of the glycine cleavage system by branched-chain amino acid metabolites.", "content": "The effects of 18 normally occurring and 11 patalogical metabolites of the branched-chain amino acids on the glycine cleavage system were investigated on intact rat liver mitochondria. It was demonstrated, that 2-oxo-isovaleric acid, 2-methyl-butyric acid, and isobutyric acid significantly inhibited the glycine cleavage system in intact mitochondria. Further studies on the solubilized glycine cleavage system demonstrated that the inhibitory effect was due to 2-methyl-butyryl-CoA (linear noncompetitive inhibition, ki: 0.1--0.15 mM) and isobutyryl-CoA (S-hyperbolic, I-linear noncompetitive inhibition, ki: 0.2--0.3 mM). Both 2-methyl-butyric acid and isobutyric acid exhibited less inhibition (2-methyl-butyric acid: competitive inhibition, ki: 5.5 mM, isobutyric acid: competitive inhibition, ki: 16 mM), while 2-oxo-isovaleric acid was without inhibitory effect, and probably affects intact mitochondria through transformation to isobutyryl-CoA. It is suggested that the inhibitory action of 2-methyl-butyryl-CoA and isobutyryl-CoA may explain the hyperglycinemia seen in propionyl-CoA carboxylase deficiency, methyl-malonyl-CoA mutase deficiency and beta-ketothiolase deficiency.", "contents": "Inhibition of the glycine cleavage system by branched-chain amino acid metabolites. The effects of 18 normally occurring and 11 patalogical metabolites of the branched-chain amino acids on the glycine cleavage system were investigated on intact rat liver mitochondria. It was demonstrated, that 2-oxo-isovaleric acid, 2-methyl-butyric acid, and isobutyric acid significantly inhibited the glycine cleavage system in intact mitochondria. Further studies on the solubilized glycine cleavage system demonstrated that the inhibitory effect was due to 2-methyl-butyryl-CoA (linear noncompetitive inhibition, ki: 0.1--0.15 mM) and isobutyryl-CoA (S-hyperbolic, I-linear noncompetitive inhibition, ki: 0.2--0.3 mM). Both 2-methyl-butyric acid and isobutyric acid exhibited less inhibition (2-methyl-butyric acid: competitive inhibition, ki: 5.5 mM, isobutyric acid: competitive inhibition, ki: 16 mM), while 2-oxo-isovaleric acid was without inhibitory effect, and probably affects intact mitochondria through transformation to isobutyryl-CoA. It is suggested that the inhibitory action of 2-methyl-butyryl-CoA and isobutyryl-CoA may explain the hyperglycinemia seen in propionyl-CoA carboxylase deficiency, methyl-malonyl-CoA mutase deficiency and beta-ketothiolase deficiency."}
{"id": "PMID:481964", "title": "Cardiopulmonary changes in the crying neonate.", "content": "To determine hemodynamic effects of crying, 12 newborn infants recovering from the respiratory distress syndrome (RDS) were studied. When crying, the range of inspiratory esophageal pressure was -18.8 to -32.5 cm H2O and the range of espiratory pressure was +6.2 to 34.4 cm H2O. The esophageal pressure remained positive for a mean value of 66% of the respiratory cycle. There was a mean significant increase in heart rate of 19 beats/min. The systolic and diastolic blood pressures increased significantly at the beginning of strain to 115 and 135% of the respective control values. There was a progressive decrease in systolic and diastolic pressures during the period of strain and the systolic pressures reached values significantly less than control. With the decrease in systolic and diastolic pressures, there were pronounced reductions in pulse pressures. Three infants reached pulse pressure values less than 1% of control when cries were sustained for nine cardiac cycles. There was a significant mean decrease in arterial oxygen tension (PaO2) of 16.8 mm Hg. There were no changes in arterial carbon dioxide tension (PaCO2), pH, or base excess.", "contents": "Cardiopulmonary changes in the crying neonate. To determine hemodynamic effects of crying, 12 newborn infants recovering from the respiratory distress syndrome (RDS) were studied. When crying, the range of inspiratory esophageal pressure was -18.8 to -32.5 cm H2O and the range of espiratory pressure was +6.2 to 34.4 cm H2O. The esophageal pressure remained positive for a mean value of 66% of the respiratory cycle. There was a mean significant increase in heart rate of 19 beats/min. The systolic and diastolic blood pressures increased significantly at the beginning of strain to 115 and 135% of the respective control values. There was a progressive decrease in systolic and diastolic pressures during the period of strain and the systolic pressures reached values significantly less than control. With the decrease in systolic and diastolic pressures, there were pronounced reductions in pulse pressures. Three infants reached pulse pressure values less than 1% of control when cries were sustained for nine cardiac cycles. There was a significant mean decrease in arterial oxygen tension (PaO2) of 16.8 mm Hg. There were no changes in arterial carbon dioxide tension (PaCO2), pH, or base excess."}
{"id": "PMID:481966", "title": "Pulmonary function studies of conjoined thoracopagus twins.", "content": "Lung functions were measured on 13-day-old conjoined thoracopagus twins. Mean values for tidal volume and minute ventilation were 14.8 ml and 1102 ml/min for twin A and 12.8 ml and 963 ml/min for twin B. Functional residual capacities (FRC) were 35 ml/kg and 39 ml/kg for twins A and B, respectively. Pulmonary compliance and flow resistance for twin A were 2.8 ml/cm H2O and 75 cm H2O/liter.sec-1 with 45% of the total work used to overcome elastic resistance. Although values for resistance and work of breathing are within the normal range for studies reported elsewhere, these results tend to be elevated and probably reflect an abnormal state.", "contents": "Pulmonary function studies of conjoined thoracopagus twins. Lung functions were measured on 13-day-old conjoined thoracopagus twins. Mean values for tidal volume and minute ventilation were 14.8 ml and 1102 ml/min for twin A and 12.8 ml and 963 ml/min for twin B. Functional residual capacities (FRC) were 35 ml/kg and 39 ml/kg for twins A and B, respectively. Pulmonary compliance and flow resistance for twin A were 2.8 ml/cm H2O and 75 cm H2O/liter.sec-1 with 45% of the total work used to overcome elastic resistance. Although values for resistance and work of breathing are within the normal range for studies reported elsewhere, these results tend to be elevated and probably reflect an abnormal state."}
{"id": "PMID:481967", "title": "Abnormal pyruvate and alpha-ketoglutarate dehydrogenase complexes in a patient with lactic acidemia.", "content": "Activities of pyruvate decarboxylase (PDC), alpha-ketoglutarate decarboxylase (KGDC) and both the pyruvate and alpha-ketoglutarate dehydrogenase complexes (PDH complex and KGDH complex) were measured, and kinetic properties of PDC were studied in fibroblasts derived from normal individuals and from a 2-yr-old girl with congenital lactic acidemia and severe retardation of growth and development. The activities of PDC, KGDC, PDH complex, and KGDH complex in the patient were 1.12 +/- 0.12, 2.33 +/- 0.42, 9.00 +/- 0.50, and 16.46 +/- 1.57 and in controls 3.10 +/- 0.16, 5.36 +/- 0.56, 24.13 +/- 1.61 and 44.95 +/- 3.72 nmole/mg protein/hr. The optimum pH (6.0) and Michaelis constants (Km) for pyruvate of PDC (1.0-1.6 X 10(-5) M) were similar in fibroblasts of the patient and controls. PDC activity was more sensitive to denaturation by heat in the fibroblasts of the patient than those from controls, while heat denaturation curves of KGDC were similar in the patient and control. Higher concentrations of thiamine pyrophosphate (TPP) were required to protect PDC from heat denaturation in the patient. TPP was more easily removed from PDC in the patient than in the control by washing the fibroblasts with alkaline buffer. These results suggest that the PDC enzyme of the patient is in an altered molecular form, to which TPP is loosely bound. This particular constellation of abnormalities has not previously been reported in patients with lactic acidemia.", "contents": "Abnormal pyruvate and alpha-ketoglutarate dehydrogenase complexes in a patient with lactic acidemia. Activities of pyruvate decarboxylase (PDC), alpha-ketoglutarate decarboxylase (KGDC) and both the pyruvate and alpha-ketoglutarate dehydrogenase complexes (PDH complex and KGDH complex) were measured, and kinetic properties of PDC were studied in fibroblasts derived from normal individuals and from a 2-yr-old girl with congenital lactic acidemia and severe retardation of growth and development. The activities of PDC, KGDC, PDH complex, and KGDH complex in the patient were 1.12 +/- 0.12, 2.33 +/- 0.42, 9.00 +/- 0.50, and 16.46 +/- 1.57 and in controls 3.10 +/- 0.16, 5.36 +/- 0.56, 24.13 +/- 1.61 and 44.95 +/- 3.72 nmole/mg protein/hr. The optimum pH (6.0) and Michaelis constants (Km) for pyruvate of PDC (1.0-1.6 X 10(-5) M) were similar in fibroblasts of the patient and controls. PDC activity was more sensitive to denaturation by heat in the fibroblasts of the patient than those from controls, while heat denaturation curves of KGDC were similar in the patient and control. Higher concentrations of thiamine pyrophosphate (TPP) were required to protect PDC from heat denaturation in the patient. TPP was more easily removed from PDC in the patient than in the control by washing the fibroblasts with alkaline buffer. These results suggest that the PDC enzyme of the patient is in an altered molecular form, to which TPP is loosely bound. This particular constellation of abnormalities has not previously been reported in patients with lactic acidemia."}
{"id": "PMID:481968", "title": "Pulmonary vasodilator action of tolazoline.", "content": "The pulmonary vasodilator action of tolazoline in newborn lambs was shown to be mediated via histamine receptors. Maximal changes in pulmonary vascular resistance, deltaPVR, were calculated as percents of the base line value, %deltaPVR. The mean %deltaPVR after tolazoline, 1 mg/kg, was -25 +/- 4% for eight lambs. Four lambs then received the histamine H1 receptor antagonist, diphenhydramine, and the mean %deltaPVR due to tolazoline was -12 +/- 4%. Four lambs received the H2 receptor antagonist, metiamide, and the mean %delta PVR due to tolazoline was -18 +/- 5%. After both H1 and H2 antagonists, the mean %deltaPVR due to tolazoline was +6 +/- 8%. Therefore, both histamine H1 and H2 receptors were involved in the vasodilator response to tolazoline.", "contents": "Pulmonary vasodilator action of tolazoline. The pulmonary vasodilator action of tolazoline in newborn lambs was shown to be mediated via histamine receptors. Maximal changes in pulmonary vascular resistance, deltaPVR, were calculated as percents of the base line value, %deltaPVR. The mean %deltaPVR after tolazoline, 1 mg/kg, was -25 +/- 4% for eight lambs. Four lambs then received the histamine H1 receptor antagonist, diphenhydramine, and the mean %deltaPVR due to tolazoline was -12 +/- 4%. Four lambs received the H2 receptor antagonist, metiamide, and the mean %delta PVR due to tolazoline was -18 +/- 5%. After both H1 and H2 antagonists, the mean %deltaPVR due to tolazoline was +6 +/- 8%. Therefore, both histamine H1 and H2 receptors were involved in the vasodilator response to tolazoline."}
{"id": "PMID:481969", "title": "Quantitative noninvasive method to measure cerebral blood flow in newborn infants.", "content": "We measured cerebral blood flow (CBF) in 32 healthy neonates by venous occlusion plethysmography. Mean CBF was 63 ml/min/100 gm which compared favorably with invasive methods used in older children and adult subjects. We suggest that this is a useful method to quantify CBF in neonates. It may be valuable in assessing sequential changes occurring during asphyxia, intracranial hemorrhage, or during administration of various gas mixtures and drugs such as theophylline.", "contents": "Quantitative noninvasive method to measure cerebral blood flow in newborn infants. We measured cerebral blood flow (CBF) in 32 healthy neonates by venous occlusion plethysmography. Mean CBF was 63 ml/min/100 gm which compared favorably with invasive methods used in older children and adult subjects. We suggest that this is a useful method to quantify CBF in neonates. It may be valuable in assessing sequential changes occurring during asphyxia, intracranial hemorrhage, or during administration of various gas mixtures and drugs such as theophylline."}
{"id": "PMID:481970", "title": "Fatal Haemophilus influenzae endocarditis diagnosed by echocardiography in an infant.", "content": "A 1-year-old boy was admitted to the hospital with Haemophilus influenzae meningitis. On the second hospital day a heart murmur heard on admission seemed louder. No signs of congestive heart failure were present. An echocardiogram demonstrated a persistent echo-dense mass behind the anterior leaflet of the mitral valve. A presumptive diagnosis of endocarditis was made. After ten days of antibiotic treatment the child was considerably improved. Treatment was continued for endocarditis. Over the next several days the infant's condition deteriorated. On the 16th hospital day he died. Autopsy examination showed a large vegetation attached to the posterior mitral valve leaflet. Bacterial endocarditis caused by H influenzae is uncommon at any age and rare in children. Echocardiography provided a definitive diagnosis early in the hospital course of this patient.", "contents": "Fatal Haemophilus influenzae endocarditis diagnosed by echocardiography in an infant. A 1-year-old boy was admitted to the hospital with Haemophilus influenzae meningitis. On the second hospital day a heart murmur heard on admission seemed louder. No signs of congestive heart failure were present. An echocardiogram demonstrated a persistent echo-dense mass behind the anterior leaflet of the mitral valve. A presumptive diagnosis of endocarditis was made. After ten days of antibiotic treatment the child was considerably improved. Treatment was continued for endocarditis. Over the next several days the infant's condition deteriorated. On the 16th hospital day he died. Autopsy examination showed a large vegetation attached to the posterior mitral valve leaflet. Bacterial endocarditis caused by H influenzae is uncommon at any age and rare in children. Echocardiography provided a definitive diagnosis early in the hospital course of this patient."}
{"id": "PMID:481972", "title": "Risk factors associated with failure to receive vaccinations.", "content": "A major purpose of a state-wide survey to document the vaccination status of 1,003 2-year-old children was to identify factors associated with failure to receive the recommended vaccinations. With a basic series of immunization defined as three doses of diphtheria-tetanus-pertussis (DTP), three oral polio vaccine (OPV), one measles, and one rubella, 72.5% of the children had completed the series. When the completed series was redefined to include a fourth DTP and mumps vaccine the rate of completion dropped to 40.8%. However, 59.1% of the children who had not completed this optimal series could be brought up-to-date with a single visit to their provider of medical care. Demographic variables independently associated with completion of the basic series were increased paternal education (P less than .001), increased maternal education (P less than .02), smaller family size (P less than .01) and higher socioeconomic status, as determined by census tract or rural town of residence (P less than .02). Race was not found to be a factor associated with vaccination rates when socioeconomic status was controlled. Patients who received their vaccinations from private physicians had a better vaccination rate than those who attended health department clinics. This difference persisted even when socioeconomic status was controlled by residence (P less than .02). The simultaneous comparison of parental education and family size demonstrated that a child having one parent with less than 12 years education or having at least three siblings has a fourfold greater risk of failure to complete his immunization than children whose parents are both college graduates. By using paternal and maternal education level and family size as screening variables, children at high risk for failure to complete their immunizations could be identified prospectively and made the target of intervention programs to improve compliance.", "contents": "Risk factors associated with failure to receive vaccinations. A major purpose of a state-wide survey to document the vaccination status of 1,003 2-year-old children was to identify factors associated with failure to receive the recommended vaccinations. With a basic series of immunization defined as three doses of diphtheria-tetanus-pertussis (DTP), three oral polio vaccine (OPV), one measles, and one rubella, 72.5% of the children had completed the series. When the completed series was redefined to include a fourth DTP and mumps vaccine the rate of completion dropped to 40.8%. However, 59.1% of the children who had not completed this optimal series could be brought up-to-date with a single visit to their provider of medical care. Demographic variables independently associated with completion of the basic series were increased paternal education (P less than .001), increased maternal education (P less than .02), smaller family size (P less than .01) and higher socioeconomic status, as determined by census tract or rural town of residence (P less than .02). Race was not found to be a factor associated with vaccination rates when socioeconomic status was controlled. Patients who received their vaccinations from private physicians had a better vaccination rate than those who attended health department clinics. This difference persisted even when socioeconomic status was controlled by residence (P less than .02). The simultaneous comparison of parental education and family size demonstrated that a child having one parent with less than 12 years education or having at least three siblings has a fourfold greater risk of failure to complete his immunization than children whose parents are both college graduates. By using paternal and maternal education level and family size as screening variables, children at high risk for failure to complete their immunizations could be identified prospectively and made the target of intervention programs to improve compliance."}
{"id": "PMID:481973", "title": "Experimental pneumothorax detected by thermography.", "content": "After induction of pneumothorax in rabbits, thermograms showed decreased heat emission from the involved side. These observations suggest that noninvasive monitors of thoracic wall temperatures might be useful for early detection of air-leak phenomenon in high-risk neonates.", "contents": "Experimental pneumothorax detected by thermography. After induction of pneumothorax in rabbits, thermograms showed decreased heat emission from the involved side. These observations suggest that noninvasive monitors of thoracic wall temperatures might be useful for early detection of air-leak phenomenon in high-risk neonates."}
{"id": "PMID:481974", "title": "Refractory hypoglycemia associated with a malpositioned umbilical artery catheter.", "content": "An infant with unexplained hypoglycemia who responded poorly to medical management had the tip of his umbilical artery catheter positioned near the major arteries supplying the pancreas. Catheter repositioning resulted in immediate normoglycemia. A \"reactive\" response to the direct infusion of glucose is postulated on the basis of the clinical response and a decrease in serum insulin/blood glucose ratio.", "contents": "Refractory hypoglycemia associated with a malpositioned umbilical artery catheter. An infant with unexplained hypoglycemia who responded poorly to medical management had the tip of his umbilical artery catheter positioned near the major arteries supplying the pancreas. Catheter repositioning resulted in immediate normoglycemia. A \"reactive\" response to the direct infusion of glucose is postulated on the basis of the clinical response and a decrease in serum insulin/blood glucose ratio."}
{"id": "PMID:481975", "title": "The effect of head position and angle of tracheal bifurcation on bronchus catheterization in the intubated neonate.", "content": "The effect of head position and angle of tracheal bifurcation on bronchus catheterization was studied in newborn infants. Frequency of bronchus catheterization following appropriate head positioning was evaluated radiographically after passage of a No. 5 French feeding tube in eight intubated neonates within 30 minutes post mortem. Positioning the infants head to the right in preparation for a suctioning procedure may facilitate but not assure catheterization of the left main stem bronchus (LMSB). Positioning the infant's head straight or rotating to the left appears to be equally effective in catheterization of the right main stem bronchus (RMSB). The predominance of RMSB catheterization in infants with their head in the straight position may be explained by the different angles of mainstem bronchi divergence which we determined using radiographs. The angles of divergence of the LMSB and the RMSB from the trachea averaged 44.4 degrees and 24.1 degrees, respectively.", "contents": "The effect of head position and angle of tracheal bifurcation on bronchus catheterization in the intubated neonate. The effect of head position and angle of tracheal bifurcation on bronchus catheterization was studied in newborn infants. Frequency of bronchus catheterization following appropriate head positioning was evaluated radiographically after passage of a No. 5 French feeding tube in eight intubated neonates within 30 minutes post mortem. Positioning the infants head to the right in preparation for a suctioning procedure may facilitate but not assure catheterization of the left main stem bronchus (LMSB). Positioning the infant's head straight or rotating to the left appears to be equally effective in catheterization of the right main stem bronchus (RMSB). The predominance of RMSB catheterization in infants with their head in the straight position may be explained by the different angles of mainstem bronchi divergence which we determined using radiographs. The angles of divergence of the LMSB and the RMSB from the trachea averaged 44.4 degrees and 24.1 degrees, respectively."}
{"id": "PMID:481976", "title": "Vitamin E and neonatal bilirubinemia.", "content": "A study was designed to determine the effect of vitamin E on bilirubinemia in the preterm infant. Twenty infants with birth weight between 1,000 and 1,500 gm and 20 infants with birth weights between 1,501 and 2,000 gm were studied. Half the infants in each birth weight group received vitamin E administered intramuscularly in a total dose of 50 mg/kg during days 1 to 3 of life; the remaining infants served as controls. The administration of vitamin E produced significantly increased plasma tocopherol concentrations and normal hydrogen peroxide hemolysis tests by the end of the first week of life. Infants with birthweights less than or equal to 1500 gm who received vitamin E demonstrated a significant decrease in serum bilirubin on day 3 of life (6.5 +/- 2.2 vs 8.8 +/- 2.2 mg/dl) as well as a significant decrease in peak serum bilirubin during the first week of life (8.3 +/- 2.2 vs 10.6 +/- 2.6 mg/dl). The duration of phototherapy also was significantly less in the vitamin E-supplemented group (48 +/- 18 vs 107 +/- 31 hours). These differences were less pronounced in infants with birth weights more than 1,500 gm.", "contents": "Vitamin E and neonatal bilirubinemia. A study was designed to determine the effect of vitamin E on bilirubinemia in the preterm infant. Twenty infants with birth weight between 1,000 and 1,500 gm and 20 infants with birth weights between 1,501 and 2,000 gm were studied. Half the infants in each birth weight group received vitamin E administered intramuscularly in a total dose of 50 mg/kg during days 1 to 3 of life; the remaining infants served as controls. The administration of vitamin E produced significantly increased plasma tocopherol concentrations and normal hydrogen peroxide hemolysis tests by the end of the first week of life. Infants with birthweights less than or equal to 1500 gm who received vitamin E demonstrated a significant decrease in serum bilirubin on day 3 of life (6.5 +/- 2.2 vs 8.8 +/- 2.2 mg/dl) as well as a significant decrease in peak serum bilirubin during the first week of life (8.3 +/- 2.2 vs 10.6 +/- 2.6 mg/dl). The duration of phototherapy also was significantly less in the vitamin E-supplemented group (48 +/- 18 vs 107 +/- 31 hours). These differences were less pronounced in infants with birth weights more than 1,500 gm."}
{"id": "PMID:481978", "title": "Nonimmune hydrops fetalis: report of 22 cases including three siblings.", "content": "Twenty-two cases of nonimmune hydrops fetalis (NIHF) seen over a three-year period are described. Eight cases were associated with major congenital anomalies, seven cases with minor anomalies or other disease entities, and seven idiopathic cases. The overall mortality rate was 50%, greatest in those cases complicated by major anomalies. The clinical problems commonly encountered in management of these patients are reviewed, and include prenatal detection, perinatal asphyxia, disseminated intravascular coagulopathy, fluid and electrolyte imbalance, and respiratory difficulty. A review of the literature for those entities found in association with NIHF is also included. The case studies of three consecutive siblings with NIHF born to the same parents are briefly analyzed.", "contents": "Nonimmune hydrops fetalis: report of 22 cases including three siblings. Twenty-two cases of nonimmune hydrops fetalis (NIHF) seen over a three-year period are described. Eight cases were associated with major congenital anomalies, seven cases with minor anomalies or other disease entities, and seven idiopathic cases. The overall mortality rate was 50%, greatest in those cases complicated by major anomalies. The clinical problems commonly encountered in management of these patients are reviewed, and include prenatal detection, perinatal asphyxia, disseminated intravascular coagulopathy, fluid and electrolyte imbalance, and respiratory difficulty. A review of the literature for those entities found in association with NIHF is also included. The case studies of three consecutive siblings with NIHF born to the same parents are briefly analyzed."}
{"id": "PMID:481979", "title": "Folic acid supplementation in low birth weight infants.", "content": "Low birth weight infants (246) entered a trial to folic acid supplementation from 3 weeks to 12 months of age. The folic acid group had significantly higher mean hemoglobin levels at 6 and 9 months of age but the differences were only about 0.5 gm/dl, there was no significant difference in hematocrit, and in both groups of infants the mean hemoglobin levels were higher than those of normal birth weight infants. The differences in hemoglobin, although statistically significant, are of uncertain clinical significance. Median red cell folate levels remained within the normal adult range in both groups of infants. A minority of infants in the untreated group had low red cell folate levels but this was usually temporary, corrected by dietary folate, and not associated with low hemoglobin. Weight gain was not affected by folic acid supplementation. The infants in this trial were fed with a milk preparation containing 3.5 microgram/100 ml of folic acid which is a similar concentration to that of human milk and we recommend that the folate content of milks fed to low birth weight infants should not fall below this level. We do not have sufficient grounds to recommend routine folic acid supplements for all low birth weight infants throughout the first year of life but there is a possibility that their folate intake may sometimes be suboptimal.", "contents": "Folic acid supplementation in low birth weight infants. Low birth weight infants (246) entered a trial to folic acid supplementation from 3 weeks to 12 months of age. The folic acid group had significantly higher mean hemoglobin levels at 6 and 9 months of age but the differences were only about 0.5 gm/dl, there was no significant difference in hematocrit, and in both groups of infants the mean hemoglobin levels were higher than those of normal birth weight infants. The differences in hemoglobin, although statistically significant, are of uncertain clinical significance. Median red cell folate levels remained within the normal adult range in both groups of infants. A minority of infants in the untreated group had low red cell folate levels but this was usually temporary, corrected by dietary folate, and not associated with low hemoglobin. Weight gain was not affected by folic acid supplementation. The infants in this trial were fed with a milk preparation containing 3.5 microgram/100 ml of folic acid which is a similar concentration to that of human milk and we recommend that the folate content of milks fed to low birth weight infants should not fall below this level. We do not have sufficient grounds to recommend routine folic acid supplements for all low birth weight infants throughout the first year of life but there is a possibility that their folate intake may sometimes be suboptimal."}
{"id": "PMID:481980", "title": "Hyperammonemia associated with perinatal asphyxia.", "content": "Twelve infants with severe perinatal asphyxia were found to have elevated blood ammonia levels (302 to 960 microgram/100 ml). In the seven survivors, hyperammonemia was associated with CNS irritability, hyperthermia, hypertension, and wide neonatal heart rate oscillations. Follow-up examinations revealed severe neurologic dysfunction in five of seven infants. CNS depression, hyperthermia, hypertension, and a nonreactive, fixed heart rate characterized the infants that died. These findings suggest a clinical entity secondary to perinatal asphyxia whose signs and symptoms may be related to hyperammonemia.", "contents": "Hyperammonemia associated with perinatal asphyxia. Twelve infants with severe perinatal asphyxia were found to have elevated blood ammonia levels (302 to 960 microgram/100 ml). In the seven survivors, hyperammonemia was associated with CNS irritability, hyperthermia, hypertension, and wide neonatal heart rate oscillations. Follow-up examinations revealed severe neurologic dysfunction in five of seven infants. CNS depression, hyperthermia, hypertension, and a nonreactive, fixed heart rate characterized the infants that died. These findings suggest a clinical entity secondary to perinatal asphyxia whose signs and symptoms may be related to hyperammonemia."}
{"id": "PMID:481982", "title": "Neurologic abnormalities in near miss for sudden infant death syndrome infants.", "content": "As part of the Sudden Death Research Project at Stanford University School of Medicine, we have performed systematic neurologic examination of 41 near miss infants, 7 normal siblings of babies who died a sudden infant death, and 21 normal control infants. Many infants were examined longitudinally until 2 years of age. We found that near miss infants under 3 months of age had consistent abnormalities of muscle tone, particularly shoulder hypotonia. These abnormalities disappeared with maturation, but only 60% of older infants who had suffered near miss episodes of neurologically and developmentally normal. In addition, only one of the seven apparently normal siblings had an entirely normal neurologic examination when first seen. Thus, infants who present with near miss episodes may have an underlying central nervous system abnormality or may acquire some abnormality as a result of the episode.", "contents": "Neurologic abnormalities in near miss for sudden infant death syndrome infants. As part of the Sudden Death Research Project at Stanford University School of Medicine, we have performed systematic neurologic examination of 41 near miss infants, 7 normal siblings of babies who died a sudden infant death, and 21 normal control infants. Many infants were examined longitudinally until 2 years of age. We found that near miss infants under 3 months of age had consistent abnormalities of muscle tone, particularly shoulder hypotonia. These abnormalities disappeared with maturation, but only 60% of older infants who had suffered near miss episodes of neurologically and developmentally normal. In addition, only one of the seven apparently normal siblings had an entirely normal neurologic examination when first seen. Thus, infants who present with near miss episodes may have an underlying central nervous system abnormality or may acquire some abnormality as a result of the episode."}
{"id": "PMID:482013", "title": "MMPI characteristics associated with cerebral palsy and dystonia musculorum deformans.", "content": "The origins of dystonia musculorum deformans are now considered to be organic. However, misdiagnosis of dystonia as a functional psychiatric disorder--usually conversion reaction--has persisted. The present study describes personality traits as measured by the Minnesota Multiphasic Personality Inventory in 30 persons with dystonia and in a control group of 37 persons with cerebral palsy. The data, examined by diagnosis, level of disability, and sex, showed no differences for diagnostic groups or levels of disability. Males scored in the direction of greater psychopathology than did females. The male dystonics showed the highest elevations of MMPI scales of all the groups. Although only one person with dystonia musculorum deformans and none with cerebral palsy produced the profile usually associated with conversion reaction, 36% of all profiles showed two scales above a T score of 70. This finding suggested that young adults with a physically disabling disease may be at higher risk for developing maladaptive personality traits.", "contents": "MMPI characteristics associated with cerebral palsy and dystonia musculorum deformans. The origins of dystonia musculorum deformans are now considered to be organic. However, misdiagnosis of dystonia as a functional psychiatric disorder--usually conversion reaction--has persisted. The present study describes personality traits as measured by the Minnesota Multiphasic Personality Inventory in 30 persons with dystonia and in a control group of 37 persons with cerebral palsy. The data, examined by diagnosis, level of disability, and sex, showed no differences for diagnostic groups or levels of disability. Males scored in the direction of greater psychopathology than did females. The male dystonics showed the highest elevations of MMPI scales of all the groups. Although only one person with dystonia musculorum deformans and none with cerebral palsy produced the profile usually associated with conversion reaction, 36% of all profiles showed two scales above a T score of 70. This finding suggested that young adults with a physically disabling disease may be at higher risk for developing maladaptive personality traits."}
{"id": "PMID:482015", "title": "Relationship between anxiety and performance in scuba diving.", "content": "The present research examined the relationship between the anxiety levels of 62 beginning Scuba diving students and standardized performance tests from the Y.M.C.A. training program. Results suggested that there was no relationship between anxiety and performance on relatively simple tasks, while there was a relationship between anxiety and performance on the more complex diving maneuvers.", "contents": "Relationship between anxiety and performance in scuba diving. The present research examined the relationship between the anxiety levels of 62 beginning Scuba diving students and standardized performance tests from the Y.M.C.A. training program. Results suggested that there was no relationship between anxiety and performance on relatively simple tasks, while there was a relationship between anxiety and performance on the more complex diving maneuvers."}
{"id": "PMID:482016", "title": "Longitudinal study of cognitive abilities and academic achievement.", "content": "Assessment was made of the effectiveness of a battery of cognitive tests administered in kindergarten in the prediction of sixth grade achievement in reading comprehension, spelling, language usage, and arithmetic. Cognitive abilities included several factors of intelligence, visual perception and visual sequential memory, visual-motor integration, and auditory perception and auditory sequential memory. Measures of prior learning were also included. Subjects were 58 children in a suburban public school district. A criterion for predictive utility for correlation coefficients was established, and simple correlation coefficients for various kindergarten measures and sixth grade achievement ranged from the criterion of .35 to .69. In partial correlations with the effects of ability to understand ideas expressed in words removed, correlation coefficients for various cognitive measures and achievement tests ranged from the criterion of .35 to .63. Combinations of kindergarten measures having optimal multiple correlations with later school achievement generally approached or exceeded .70. These findings are discussed and suggestions are made for further research.", "contents": "Longitudinal study of cognitive abilities and academic achievement. Assessment was made of the effectiveness of a battery of cognitive tests administered in kindergarten in the prediction of sixth grade achievement in reading comprehension, spelling, language usage, and arithmetic. Cognitive abilities included several factors of intelligence, visual perception and visual sequential memory, visual-motor integration, and auditory perception and auditory sequential memory. Measures of prior learning were also included. Subjects were 58 children in a suburban public school district. A criterion for predictive utility for correlation coefficients was established, and simple correlation coefficients for various kindergarten measures and sixth grade achievement ranged from the criterion of .35 to .69. In partial correlations with the effects of ability to understand ideas expressed in words removed, correlation coefficients for various cognitive measures and achievement tests ranged from the criterion of .35 to .63. Combinations of kindergarten measures having optimal multiple correlations with later school achievement generally approached or exceeded .70. These findings are discussed and suggestions are made for further research."}
{"id": "PMID:482018", "title": "Discrimination of infants' cry-signals.", "content": "A technique for clustering infants' cry-signals on the basis of perceptually discriminable acoustic characteristics is reported. A cry-sequence containing all pairwise combinations of 24 signals was constructed. These signals have been used previously in cry-recognition studies (e.g., Wasz-Hockert, et al., 1968) and consist of six each of pain, hunger, pleasure, and birth. 20 musically competent subjects were tested individually and instructed to compare each cry with the one immediately preceding it in the sequence. Forced-choice \"similarity\" scores were summed for every pair of cries. These \"similarity scores\" were data for both a manual and a computerized clustering method. There was absolute agreement between these two methods, and the clusters of cry-signals correspond very closely to the four original cry groups. This seems a valid technique for examining the acoustic similarity of infants' cry-signals.", "contents": "Discrimination of infants' cry-signals. A technique for clustering infants' cry-signals on the basis of perceptually discriminable acoustic characteristics is reported. A cry-sequence containing all pairwise combinations of 24 signals was constructed. These signals have been used previously in cry-recognition studies (e.g., Wasz-Hockert, et al., 1968) and consist of six each of pain, hunger, pleasure, and birth. 20 musically competent subjects were tested individually and instructed to compare each cry with the one immediately preceding it in the sequence. Forced-choice \"similarity\" scores were summed for every pair of cries. These \"similarity scores\" were data for both a manual and a computerized clustering method. There was absolute agreement between these two methods, and the clusters of cry-signals correspond very closely to the four original cry groups. This seems a valid technique for examining the acoustic similarity of infants' cry-signals."}
{"id": "PMID:482020", "title": "ITPA interchannel differences.", "content": "The extent to which auditory and visual channel part-score means differ typically was studied by reference to the performance of 962 average children in the normative sample of the Illinois Test of Psycholinguistic Abilities. A standard error of measurement difference of 1.87 scaled score points was obtained, such indicating comparatively little difference in the mean performance of average children on the two communication channels of this test. Differences between means of auditory and visual channels greater than 3.09 or 4.36 scaled score points are presented as statistically significant at the 5 or 1 percent level of confidence, respectively. Significant part-score differences suggest atypical performance in the channel with the lesser mean scaled score and may indicate the presence of a disability among behaviors assessed by subtests within that channel.", "contents": "ITPA interchannel differences. The extent to which auditory and visual channel part-score means differ typically was studied by reference to the performance of 962 average children in the normative sample of the Illinois Test of Psycholinguistic Abilities. A standard error of measurement difference of 1.87 scaled score points was obtained, such indicating comparatively little difference in the mean performance of average children on the two communication channels of this test. Differences between means of auditory and visual channels greater than 3.09 or 4.36 scaled score points are presented as statistically significant at the 5 or 1 percent level of confidence, respectively. Significant part-score differences suggest atypical performance in the channel with the lesser mean scaled score and may indicate the presence of a disability among behaviors assessed by subtests within that channel."}
{"id": "PMID:482021", "title": "Kinesthetic augmentation and reduction in adult females.", "content": "The Petrie Block Test was used to measure kinesthetic after-effect in a sample of 60 normal adult female subjects. In addition to the establishment of normative data for this sample, several procedural questions raised by other investigators were addressed. It was determined that the use of ascending trials produced more accurate baseline measures than the alternate use of ascending and descending trials. Also, order of test presentation affected baseline measures. Since a rather large proportion of this sample was comprised of stimulus-governed individuals, some factors related to their particular perceptual style were examined.", "contents": "Kinesthetic augmentation and reduction in adult females. The Petrie Block Test was used to measure kinesthetic after-effect in a sample of 60 normal adult female subjects. In addition to the establishment of normative data for this sample, several procedural questions raised by other investigators were addressed. It was determined that the use of ascending trials produced more accurate baseline measures than the alternate use of ascending and descending trials. Also, order of test presentation affected baseline measures. Since a rather large proportion of this sample was comprised of stimulus-governed individuals, some factors related to their particular perceptual style were examined."}
{"id": "PMID:482022", "title": "Effects of clinical dosage levels of methylphenidate on two-flash thresholds and perceptual motor performance in hyperactive children.", "content": "Three groups (ns = 10) of children (normals, hyperactives, hyperactives on medication) were administered eight perceptual-motor tasks. The normal controls were significantly superior in performance in comparison with the hyperactives taken off methylphenidate on three tasks but only superior on one task (Digit Symbol) when compared to hyperactives on methylphenidate. The hyperactives on medication were also significantly superior to the hyperactives not on medication on the Digit Symbol task.", "contents": "Effects of clinical dosage levels of methylphenidate on two-flash thresholds and perceptual motor performance in hyperactive children. Three groups (ns = 10) of children (normals, hyperactives, hyperactives on medication) were administered eight perceptual-motor tasks. The normal controls were significantly superior in performance in comparison with the hyperactives taken off methylphenidate on three tasks but only superior on one task (Digit Symbol) when compared to hyperactives on methylphenidate. The hyperactives on medication were also significantly superior to the hyperactives not on medication on the Digit Symbol task."}
{"id": "PMID:482023", "title": "Aesthetic dimensions and picture memory: a structural re-analysis.", "content": "The data of Franken and Davis (1975) on the prediction of memory for pictures from aesthetic ratings were re-analyzed, employing smallest space analysis to explore the structural aspects of memory and the aesthetic dimensions. The major results of this re-analysis identified one relatively pervasive dimension, distinctiveness, which encompassed most of the memorial and aesthetic variables.", "contents": "Aesthetic dimensions and picture memory: a structural re-analysis. The data of Franken and Davis (1975) on the prediction of memory for pictures from aesthetic ratings were re-analyzed, employing smallest space analysis to explore the structural aspects of memory and the aesthetic dimensions. The major results of this re-analysis identified one relatively pervasive dimension, distinctiveness, which encompassed most of the memorial and aesthetic variables."}
{"id": "PMID:482024", "title": "Beliefs and anticipated situations influencing intentions to use drugs.", "content": "Unique differences between usage segments in beliefs, perceptions, and anticipated situational influences regarding the salient dimensions underlying motivations to use marijuana are described. An extended model of behavioral intention is used for examining and explaining motivations to use marijuana in an attempt to replicate and extend recent research on drugs. Respondents' opinions concerning the interventing influences of external situations on the intentions-behavior relationship are also addressed. Implications for public policymakers concerned with drug-related social issues are also provided.", "contents": "Beliefs and anticipated situations influencing intentions to use drugs. Unique differences between usage segments in beliefs, perceptions, and anticipated situational influences regarding the salient dimensions underlying motivations to use marijuana are described. An extended model of behavioral intention is used for examining and explaining motivations to use marijuana in an attempt to replicate and extend recent research on drugs. Respondents' opinions concerning the interventing influences of external situations on the intentions-behavior relationship are also addressed. Implications for public policymakers concerned with drug-related social issues are also provided."}
{"id": "PMID:482025", "title": "Effects of training versus non-training in identification of infant cry-signals: a longitudinal study.", "content": "The effects of training on the ability to identify 4 infants' cry-signals (birth, hunger, pain, and pleasure) were studied in 60 college students (aged 18 to 30 yr.). Subjects with training scored significantly higher than those without in identifying all but the pleasure cry-signal. In a follow-up retest 10 wk. later, scores for the birth and hunger cry-signals improved significantly (p less than .05) for subjects without training, while scores for subjects with training remained about the same. The suggestion that repeated exposure to cry-signals may serve as training was discussed.", "contents": "Effects of training versus non-training in identification of infant cry-signals: a longitudinal study. The effects of training on the ability to identify 4 infants' cry-signals (birth, hunger, pain, and pleasure) were studied in 60 college students (aged 18 to 30 yr.). Subjects with training scored significantly higher than those without in identifying all but the pleasure cry-signal. In a follow-up retest 10 wk. later, scores for the birth and hunger cry-signals improved significantly (p less than .05) for subjects without training, while scores for subjects with training remained about the same. The suggestion that repeated exposure to cry-signals may serve as training was discussed."}
{"id": "PMID:482026", "title": "Changes in heart rate following eyes-closed alpha enhancement.", "content": "Subjects (n = 20 females 18 to 26 yr. old) who had been trained in an eyes-closed alpha-enhancement task were grouped according to performance and changes in heart rate were compared. Subjects who showed a facility for producing alpha also evidenced a small decrease in heart rate while a small increase in heart rate while a small increase in heart rate was shown by the low alpha-output group.", "contents": "Changes in heart rate following eyes-closed alpha enhancement. Subjects (n = 20 females 18 to 26 yr. old) who had been trained in an eyes-closed alpha-enhancement task were grouped according to performance and changes in heart rate were compared. Subjects who showed a facility for producing alpha also evidenced a small decrease in heart rate while a small increase in heart rate while a small increase in heart rate was shown by the low alpha-output group."}
{"id": "PMID:482028", "title": "Role of contextual cues in determining transfer of oddity rule in children.", "content": "60 kindergarten and 60 first grade children were trained on an oddity problem with perceptual (P) or conceptual (C) attribute for a fixed number of trials (30). After mastering the training problem, the subjects were randomly assigned to either an intradimensional (CC or PP group) or an extradimensional (CP or PC group) oddity shift. The transfer performance showed CC greater than PC greater than PP = CP group. These differences were attributed to the pattern hypotheses sampled at the start of transfer. Subjects who had acquired a conceptual oddity rule in the training had a strong tendency to sample hypotheses by the prior rule, but subjects under conditions where a perceptual-oddity rule was relevant tended to make a shift to a cue from new (conceptual oddity) rule. The older children yielded more of such a sampling mode than the younger children did, and the former were easily able to abandon a disconfirmed rule and sample hypotheses from the relevant rule domain.", "contents": "Role of contextual cues in determining transfer of oddity rule in children. 60 kindergarten and 60 first grade children were trained on an oddity problem with perceptual (P) or conceptual (C) attribute for a fixed number of trials (30). After mastering the training problem, the subjects were randomly assigned to either an intradimensional (CC or PP group) or an extradimensional (CP or PC group) oddity shift. The transfer performance showed CC greater than PC greater than PP = CP group. These differences were attributed to the pattern hypotheses sampled at the start of transfer. Subjects who had acquired a conceptual oddity rule in the training had a strong tendency to sample hypotheses by the prior rule, but subjects under conditions where a perceptual-oddity rule was relevant tended to make a shift to a cue from new (conceptual oddity) rule. The older children yielded more of such a sampling mode than the younger children did, and the former were easily able to abandon a disconfirmed rule and sample hypotheses from the relevant rule domain."}
{"id": "PMID:482030", "title": "Spatial-temporal structure of coincident-timing responses.", "content": "The spatial temporal structure of coincident timing responses was investigated using an 86-cm movement from a microswitch to a barrier. The speed of the movement was monitored by a series of photocells placed at equal intervals along the movement line. Subjects (N = 24) watched a timer and attempted to displace the barrier at the precise moment that the timer sweep-hand reached a \"target position\" (250, 500, 1000 msec.) All subjects were given 100 trials of practice at a particular target position. The results indicate that rapid responses have similar spatial temporal structures but that this pattern is altered in slower movements.", "contents": "Spatial-temporal structure of coincident-timing responses. The spatial temporal structure of coincident timing responses was investigated using an 86-cm movement from a microswitch to a barrier. The speed of the movement was monitored by a series of photocells placed at equal intervals along the movement line. Subjects (N = 24) watched a timer and attempted to displace the barrier at the precise moment that the timer sweep-hand reached a \"target position\" (250, 500, 1000 msec.) All subjects were given 100 trials of practice at a particular target position. The results indicate that rapid responses have similar spatial temporal structures but that this pattern is altered in slower movements."}
{"id": "PMID:482031", "title": "Relationships of curiosity to perceptual and verbal fluency in young children.", "content": "The relations of curiosity measured separately by teachers' rating and a revised object-curiosity task to verbal fluency and originality, measured by a work fluency test, and to perceptual fluency, obtained from the \"Visual Closure\" test of the Illinois Test of Psycholinguistic Abilities, were examined in 60 5- to 6-year-old boys. Although teachers' ratings of curiosity were contaminated by MA, they were significantly related to fluency in verbal recall and less strongly to fluency in visual exploration and verbal originality even when wht effect of MA was held constant. Object curiosity, which was negatively correlated with MA, showed at best negligible covariation with these three measures.", "contents": "Relationships of curiosity to perceptual and verbal fluency in young children. The relations of curiosity measured separately by teachers' rating and a revised object-curiosity task to verbal fluency and originality, measured by a work fluency test, and to perceptual fluency, obtained from the \"Visual Closure\" test of the Illinois Test of Psycholinguistic Abilities, were examined in 60 5- to 6-year-old boys. Although teachers' ratings of curiosity were contaminated by MA, they were significantly related to fluency in verbal recall and less strongly to fluency in visual exploration and verbal originality even when wht effect of MA was held constant. Object curiosity, which was negatively correlated with MA, showed at best negligible covariation with these three measures."}
{"id": "PMID:482032", "title": "Signal detection analysis of effect of white noise intensity on sensitivity to visual flicker.", "content": "Rating scale estimates of sensitivity to visual flicker were obtained from three subjects under 10 different intensities of auditory stimulation. Results indicated reliable \"sawtooth\"-like changes in sensitivity as a function of increasing intensity of white noise. No systematic and reliable changes were found in estimates of response bias. Theory and future research are discussed with reference to the possible contribution of cortical arousal.", "contents": "Signal detection analysis of effect of white noise intensity on sensitivity to visual flicker. Rating scale estimates of sensitivity to visual flicker were obtained from three subjects under 10 different intensities of auditory stimulation. Results indicated reliable \"sawtooth\"-like changes in sensitivity as a function of increasing intensity of white noise. No systematic and reliable changes were found in estimates of response bias. Theory and future research are discussed with reference to the possible contribution of cortical arousal."}
{"id": "PMID:482033", "title": "Paradoxical complementarity in the esthetic preferences of the cerebral hemispheres: an exploratory study.", "content": "Significant differences in the consistency of intrahemispheric and interhemispheric preferences indicate reliable differences in the esthetic choices associated with each hemisphere. Given an initial like or dislike of stimuli by the hemisphere first activated, the other hemisphere will exhibit preferences in the opposite direction. This suggests a process of paradoxical complementarity between the hemispheres in which the preferences of the hemispheres are antithetical but not fixed.", "contents": "Paradoxical complementarity in the esthetic preferences of the cerebral hemispheres: an exploratory study. Significant differences in the consistency of intrahemispheric and interhemispheric preferences indicate reliable differences in the esthetic choices associated with each hemisphere. Given an initial like or dislike of stimuli by the hemisphere first activated, the other hemisphere will exhibit preferences in the opposite direction. This suggests a process of paradoxical complementarity between the hemispheres in which the preferences of the hemispheres are antithetical but not fixed."}
{"id": "PMID:482034", "title": "Modality-specific effects on discrimination of short empty time intervals.", "content": "The ability of human subjects to judge the duration of short empty time intervals was studied in relation to the modality composition of the marker signals. At each trial, a pair of empty intervals was presented by a series of three successive stimuli, and the subject was asked to point out the longer interval of the two. Tone pips and flashes of light were used as the bounding signals. All the possible combinations of auditory and visual stimuli were used, in random order, to delimit pairs of intervals. Performance was found modality-independent when the first two stimuli were of the same modality. Strong response biases were introduced by varying the modality of the first or the second stimulus. Analysis of these biases indicates that memorization of the empty time intervals is affected by the modality of the binding signals.", "contents": "Modality-specific effects on discrimination of short empty time intervals. The ability of human subjects to judge the duration of short empty time intervals was studied in relation to the modality composition of the marker signals. At each trial, a pair of empty intervals was presented by a series of three successive stimuli, and the subject was asked to point out the longer interval of the two. Tone pips and flashes of light were used as the bounding signals. All the possible combinations of auditory and visual stimuli were used, in random order, to delimit pairs of intervals. Performance was found modality-independent when the first two stimuli were of the same modality. Strong response biases were introduced by varying the modality of the first or the second stimulus. Analysis of these biases indicates that memorization of the empty time intervals is affected by the modality of the binding signals."}
{"id": "PMID:482035", "title": "Changing role of stimulus intensity as a determinant of infants' attention.", "content": "When pairs of stimuli which varied in size or brightness and in pattern were presented to 6-, 9- and 24-wk.-olds, the visual attention of the younger infants appeared to be more influenced by size or brightness than by pattern, while the opposite was the case for the oldest infants. The results of another study suggested that the effects of size and brightness were additive for 9-wk.-olds. Finally, a third study using a subject-control procedure with successive stimulus presentation yielded results which were essentially the same as those of the first study. The results were interpreted as supporting the hypothesis that amount of stimulation is a major determinant of attention in the first 2 mo. and that its effectiveness decreases with age.", "contents": "Changing role of stimulus intensity as a determinant of infants' attention. When pairs of stimuli which varied in size or brightness and in pattern were presented to 6-, 9- and 24-wk.-olds, the visual attention of the younger infants appeared to be more influenced by size or brightness than by pattern, while the opposite was the case for the oldest infants. The results of another study suggested that the effects of size and brightness were additive for 9-wk.-olds. Finally, a third study using a subject-control procedure with successive stimulus presentation yielded results which were essentially the same as those of the first study. The results were interpreted as supporting the hypothesis that amount of stimulation is a major determinant of attention in the first 2 mo. and that its effectiveness decreases with age."}
{"id": "PMID:482036", "title": "Dimensionality of body perception and personality.", "content": "Fisher's Body Focus Questionnaire (1970) permits an estimation of the relative perceptive importance of eight zones of the body. The scores obtained by 377 French students of both sexes on the French version of this questionnaire show that the various body perceptions are structured by two main perceptual patterns, one of which is probably linked to orality and the other to anality. The relations which appeared between the scores and various personality traits are discussed.", "contents": "Dimensionality of body perception and personality. Fisher's Body Focus Questionnaire (1970) permits an estimation of the relative perceptive importance of eight zones of the body. The scores obtained by 377 French students of both sexes on the French version of this questionnaire show that the various body perceptions are structured by two main perceptual patterns, one of which is probably linked to orality and the other to anality. The relations which appeared between the scores and various personality traits are discussed."}
{"id": "PMID:482037", "title": "Effect of various sensory inputs on prose learning.", "content": "A pretest-posttest design was used to determine the effects of auditory input, visual input, auditory-visual input, and no input on prose learning. The principal result of a multivariate analysis of covariance combained with a multiple-comparison test was a significant difference in favor of auditory input. This difference seemed to be confined to definition items which were the substance of the input as opposed to application items which were not involved explicitly in the input. A difference between the present study and earlier ones was that a shorter exposure of stimuli was employed in the present study, which may have led to results favoring auditory input in contrast to earlier findings favoring visual input.", "contents": "Effect of various sensory inputs on prose learning. A pretest-posttest design was used to determine the effects of auditory input, visual input, auditory-visual input, and no input on prose learning. The principal result of a multivariate analysis of covariance combained with a multiple-comparison test was a significant difference in favor of auditory input. This difference seemed to be confined to definition items which were the substance of the input as opposed to application items which were not involved explicitly in the input. A difference between the present study and earlier ones was that a shorter exposure of stimuli was employed in the present study, which may have led to results favoring auditory input in contrast to earlier findings favoring visual input."}
{"id": "PMID:482038", "title": "Biofeedback and relaxation effects in electromyographic biofeedback training: a methodological note.", "content": "The present study documented progressive reductions of electromyographic potentials in a biofeedback study involving instructions to 11 female and 5 male college students to both increase and decrease the feedback (and therefore the muscle tension). When only pre-experimental baselines were employed, electromyographic reductions were found with instructions to decrease the feedback. When repeated, nontreatment baseline conditions were employed as references for analysis, no electromyographic reductions were detected beyond the progressive reductions that occurred during those baselines.", "contents": "Biofeedback and relaxation effects in electromyographic biofeedback training: a methodological note. The present study documented progressive reductions of electromyographic potentials in a biofeedback study involving instructions to 11 female and 5 male college students to both increase and decrease the feedback (and therefore the muscle tension). When only pre-experimental baselines were employed, electromyographic reductions were found with instructions to decrease the feedback. When repeated, nontreatment baseline conditions were employed as references for analysis, no electromyographic reductions were detected beyond the progressive reductions that occurred during those baselines."}
{"id": "PMID:482039", "title": "Information processing in a binary classification task.", "content": "Three choice-reaction time studies were conducted to investigate whether information processing is exhaustive or self-terminating, serial or parallel, and N-dependent or N-independent. A total of 54 subjects were required to make key-pressing responses to one, two, or three digits presented in a circular display; one key was pressed if the display contained one or more target digits and another key was pressed if the display contained only nontarget digits. The first two studies utilized within-subjects designs in which the displays were constructed from only one target and one nontarget item (Study 1) or from three target and seven nontarget items (Study 2). The third study used a between-subjects design in which different groups of subjects responded to one-, two-, or three-element displays. In general, the results indicate that CRTs increased as the total number of display elements increased and decreased as the number of target elements (or the ratio of target to nontarget items) increased for a given display size. When only target elements were presented, CRT was independent of the number of elements displayed, and when only one target was presented, CRT increased as total number of elements increased. Theses combined results are interpreted as support for the inference that information processing in visual search tasks tends to be self-terminating, serial, and N-dependent (of limited capacity).", "contents": "Information processing in a binary classification task. Three choice-reaction time studies were conducted to investigate whether information processing is exhaustive or self-terminating, serial or parallel, and N-dependent or N-independent. A total of 54 subjects were required to make key-pressing responses to one, two, or three digits presented in a circular display; one key was pressed if the display contained one or more target digits and another key was pressed if the display contained only nontarget digits. The first two studies utilized within-subjects designs in which the displays were constructed from only one target and one nontarget item (Study 1) or from three target and seven nontarget items (Study 2). The third study used a between-subjects design in which different groups of subjects responded to one-, two-, or three-element displays. In general, the results indicate that CRTs increased as the total number of display elements increased and decreased as the number of target elements (or the ratio of target to nontarget items) increased for a given display size. When only target elements were presented, CRT was independent of the number of elements displayed, and when only one target was presented, CRT increased as total number of elements increased. Theses combined results are interpreted as support for the inference that information processing in visual search tasks tends to be self-terminating, serial, and N-dependent (of limited capacity)."}
{"id": "PMID:482040", "title": "Differential validity of the WISC-R for boys and girls referred for psychological serivces.", "content": "WISC-R correlations with Reading, Spelling, and Arithmetic achievement were compared for 145 males and 69 females referred by regular classroom teachers for school psychological services. Although correlations were smaller than are typically found due to restriction of range, no significant differences occurred across sex in the magnitudes of WISC-R/achievement correlations.", "contents": "Differential validity of the WISC-R for boys and girls referred for psychological serivces. WISC-R correlations with Reading, Spelling, and Arithmetic achievement were compared for 145 males and 69 females referred by regular classroom teachers for school psychological services. Although correlations were smaller than are typically found due to restriction of range, no significant differences occurred across sex in the magnitudes of WISC-R/achievement correlations."}
{"id": "PMID:482041", "title": "Some observations on tetra-ataxiametric patterns of static balance and their relation to mental and scholastic achievements.", "content": "Recent experiences and observations gathered in the context of six independent studies in California, France, and Israel with a new method of tetra-ataxiametry based on the use of four footplates, are presented. Two independent postural parameters assessed by this method (fluctuations and anterio-posterior weight displacement) have been shown to be reliable over time and to correlate with scholastic progress at the lower elementary grades (ages 5 to 9 yr.). They also seem to be sensitive to minimal neurological dysfunction and to postural characteristics of deaf children. Results of one study indicate that diagonal balancing movements (between heel and contra-lateral toe) reflected by the tetra-ataxiametric measure of synchrony may play an important role in postural organization. Findings offer new perspectives on problems of postural laterality and on the relationship between static balance, cognition, and scholastic aptitudes at the lower grades of elementary education.", "contents": "Some observations on tetra-ataxiametric patterns of static balance and their relation to mental and scholastic achievements. Recent experiences and observations gathered in the context of six independent studies in California, France, and Israel with a new method of tetra-ataxiametry based on the use of four footplates, are presented. Two independent postural parameters assessed by this method (fluctuations and anterio-posterior weight displacement) have been shown to be reliable over time and to correlate with scholastic progress at the lower elementary grades (ages 5 to 9 yr.). They also seem to be sensitive to minimal neurological dysfunction and to postural characteristics of deaf children. Results of one study indicate that diagonal balancing movements (between heel and contra-lateral toe) reflected by the tetra-ataxiametric measure of synchrony may play an important role in postural organization. Findings offer new perspectives on problems of postural laterality and on the relationship between static balance, cognition, and scholastic aptitudes at the lower grades of elementary education."}
{"id": "PMID:482042", "title": "The Luscher Color Test: sex differences in color preference.", "content": "Sex differences in color preference on the Luscher Color Test were investigated for the green and the yellow chips. 50 male and 79 female majors in education rated and ranked each color on the popular form of the test. Females ranked and rated yellow significantly higher than did the males. Implications regarding test validity for females are discussed.", "contents": "The Luscher Color Test: sex differences in color preference. Sex differences in color preference on the Luscher Color Test were investigated for the green and the yellow chips. 50 male and 79 female majors in education rated and ranked each color on the popular form of the test. Females ranked and rated yellow significantly higher than did the males. Implications regarding test validity for females are discussed."}
{"id": "PMID:482043", "title": "A method for testing selective attention in vision.", "content": "A simple and highly flexible technique is described for testing selective attention in vision. This test has a number of advantages over techniques currently in use.", "contents": "A method for testing selective attention in vision. A simple and highly flexible technique is described for testing selective attention in vision. This test has a number of advantages over techniques currently in use."}
{"id": "PMID:482044", "title": "Dress, posture, and setting as additive factors in subjective probabilities of rape.", "content": "Subjects indicated the likelihood that a female, photographed in eight situations, would be raped. The situations were created by the Cartesian product of her dress (seductive/nonseductive), posture (aggressive/nonaggressive), and setting (inside/outside). The three variables combined additively with the highest subjective probability of rape associated with the seductive/nonaggressive/outside condition.", "contents": "Dress, posture, and setting as additive factors in subjective probabilities of rape. Subjects indicated the likelihood that a female, photographed in eight situations, would be raped. The situations were created by the Cartesian product of her dress (seductive/nonseductive), posture (aggressive/nonaggressive), and setting (inside/outside). The three variables combined additively with the highest subjective probability of rape associated with the seductive/nonaggressive/outside condition."}
{"id": "PMID:482045", "title": "Differences in laterality for recognition of nonverbal material by Japanese and English subjects.", "content": "Observed differences in hemispheric dominance of visual function, attributed to culturally based experience of Japanese and American subjects, need systematic exploration.", "contents": "Differences in laterality for recognition of nonverbal material by Japanese and English subjects. Observed differences in hemispheric dominance of visual function, attributed to culturally based experience of Japanese and American subjects, need systematic exploration."}
{"id": "PMID:482046", "title": "Ipsative vs normative interpretation of test scores: a comment on Allen and Foreman's (1976) norms on Edwards Personal Preference Schedule for female Australian therapy students.", "content": "A brief review of the literature relating to intra- vs interindividual personality assessment and the lack of logical support for the calculation of T scores using intraindividual data. It concludes with a caution regarding the interpretation of WAIS and MMPI personal profiles which are derived from interindividual scaled scores.", "contents": "Ipsative vs normative interpretation of test scores: a comment on Allen and Foreman's (1976) norms on Edwards Personal Preference Schedule for female Australian therapy students. A brief review of the literature relating to intra- vs interindividual personality assessment and the lack of logical support for the calculation of T scores using intraindividual data. It concludes with a caution regarding the interpretation of WAIS and MMPI personal profiles which are derived from interindividual scaled scores."}
{"id": "PMID:482047", "title": "Receptive phoneme sequencing knowledge of kindergarten and elementary school age children.", "content": "A new instrument was devised to investigate receptive phoneme-sequencing knowledge of a group of normally articulating children of kindergarten and elementary school age. Phoneme sequences, in the initial or final position of monosyllabic nonsense syllables that either adhered to or violated the phoneme-sequencing rules of American English, were tested. Older children correctly identified significantly more phoneme sequences than younger children. All children correctly identified significantly more impossible phoneme sequences than possible sequence items. Children did not abstract and internalize the rules for sequencing phonemes on a level comparable with that for adults until the ninth or tenth year of life. Decision-making strategies used by children to identify phoneme sequences were discussed.", "contents": "Receptive phoneme sequencing knowledge of kindergarten and elementary school age children. A new instrument was devised to investigate receptive phoneme-sequencing knowledge of a group of normally articulating children of kindergarten and elementary school age. Phoneme sequences, in the initial or final position of monosyllabic nonsense syllables that either adhered to or violated the phoneme-sequencing rules of American English, were tested. Older children correctly identified significantly more phoneme sequences than younger children. All children correctly identified significantly more impossible phoneme sequences than possible sequence items. Children did not abstract and internalize the rules for sequencing phonemes on a level comparable with that for adults until the ninth or tenth year of life. Decision-making strategies used by children to identify phoneme sequences were discussed."}
{"id": "PMID:482048", "title": "Word context effects on letter recognition.", "content": "24 subjects were asked to determine whether a target letter was present in a tachistoscopically exposed word. The presence of the target was detected faster if the target was clarly sounded in the word, that is, the target G in the word TIGER was detected faster than the target G in the word RIGHT. This effect was strongest among subjects whose over-all response time was below the median value. This result was interpreted as evidence that (1) a word is recognized as a whole before its component parts are analyzed, (2) the transformation of the word from visual to acoustic code occurs before the analysis of the individual letters, and (3) individual differences in efficiency of target detection depend on a subject's over-all response time.", "contents": "Word context effects on letter recognition. 24 subjects were asked to determine whether a target letter was present in a tachistoscopically exposed word. The presence of the target was detected faster if the target was clarly sounded in the word, that is, the target G in the word TIGER was detected faster than the target G in the word RIGHT. This effect was strongest among subjects whose over-all response time was below the median value. This result was interpreted as evidence that (1) a word is recognized as a whole before its component parts are analyzed, (2) the transformation of the word from visual to acoustic code occurs before the analysis of the individual letters, and (3) individual differences in efficiency of target detection depend on a subject's over-all response time."}
{"id": "PMID:482049", "title": "Effect of eye movement on perception of flicker.", "content": "With 3 experienced subjects CFF during eye movements was higher than that obtained by fixing a line of sight. CFF increased with a moving line of sight. With additional subjects CFF during eye movements was related to various factors such as target size and conditions of eye movement. More thorough study is required.", "contents": "Effect of eye movement on perception of flicker. With 3 experienced subjects CFF during eye movements was higher than that obtained by fixing a line of sight. CFF increased with a moving line of sight. With additional subjects CFF during eye movements was related to various factors such as target size and conditions of eye movement. More thorough study is required."}
{"id": "PMID:482050", "title": "Response mechanisms underlying kinesthetic short-term memory.", "content": "The variables of reproduction cue, activity during retention interval, and orientation of the recall movement were manipulated to assess simultaneously the viability of two hypotheses of motor short-term memory. Data partially supported hypotheses as positive response biasing was evident in recall after a filled retention interval (Pepper & Herman, 1970) and that for a given range of movements some adaption to a central reference level occurred (Laabs, 1973).", "contents": "Response mechanisms underlying kinesthetic short-term memory. The variables of reproduction cue, activity during retention interval, and orientation of the recall movement were manipulated to assess simultaneously the viability of two hypotheses of motor short-term memory. Data partially supported hypotheses as positive response biasing was evident in recall after a filled retention interval (Pepper & Herman, 1970) and that for a given range of movements some adaption to a central reference level occurred (Laabs, 1973)."}
{"id": "PMID:482052", "title": "Left-handedness: differences between student artists and scientists.", "content": "Majors in music and the visual arts had more left-handers among them than majors in the sciences. Handedness was determined by the self-reported hand used to perform a simple drawing task. Unexpectedly, a high proportion of left-handed females were in the sample.", "contents": "Left-handedness: differences between student artists and scientists. Majors in music and the visual arts had more left-handers among them than majors in the sciences. Handedness was determined by the self-reported hand used to perform a simple drawing task. Unexpectedly, a high proportion of left-handed females were in the sample."}
{"id": "PMID:482053", "title": "Role of selected encoding strategies on short-term retention of kinesthetic information.", "content": "30 volunteer subjects were required to use either a counting encoding strategy or an imagery encoding strategy to facilitate recall of information from short-term storage. Subjects were also required to use two reproducation cues in combination with two encoding strategies. Analysis indicated no differences between the accuracy at recall of subjects using imagery or counting strategies of encoding, nor was the predicted interaction between encoding strategies and reproduction cue supported.", "contents": "Role of selected encoding strategies on short-term retention of kinesthetic information. 30 volunteer subjects were required to use either a counting encoding strategy or an imagery encoding strategy to facilitate recall of information from short-term storage. Subjects were also required to use two reproducation cues in combination with two encoding strategies. Analysis indicated no differences between the accuracy at recall of subjects using imagery or counting strategies of encoding, nor was the predicted interaction between encoding strategies and reproduction cue supported."}
{"id": "PMID:482055", "title": "Effect of practice and training in spatial skills on embedded figures scores of males and females.", "content": "The effect of practice and training in spatial skills on scores obtained by male and female students on the Embedded Figures Test was examined. Forms A and B were administered 6 wk. apart to three groups of subjects (ns = 28, 27, 27) enrolled in drafting, mathematics, and liberal arts courses. During the pretest-posttest period the drafting students received training while the other two groups served as controls. Analysis indicated (1) no initial sex difference in test scores; (2) liberal arts students differed significantly from drafting and mathematics students, but there was no significant difference between the last two groups; (3) all groups improved with practice; (4) women receiving training improved more than women who did not; (5) there was a trend toward women receiving spatial training scoring more poorly than males receiving training on the pretest, but there was no significant difference on the posttest. These results suggest that sex differences in embedded-figures scores found by many previous experimenters may have been associated with differences in prior experience in spatial skills and by a confounding of sex with area of academic study.", "contents": "Effect of practice and training in spatial skills on embedded figures scores of males and females. The effect of practice and training in spatial skills on scores obtained by male and female students on the Embedded Figures Test was examined. Forms A and B were administered 6 wk. apart to three groups of subjects (ns = 28, 27, 27) enrolled in drafting, mathematics, and liberal arts courses. During the pretest-posttest period the drafting students received training while the other two groups served as controls. Analysis indicated (1) no initial sex difference in test scores; (2) liberal arts students differed significantly from drafting and mathematics students, but there was no significant difference between the last two groups; (3) all groups improved with practice; (4) women receiving training improved more than women who did not; (5) there was a trend toward women receiving spatial training scoring more poorly than males receiving training on the pretest, but there was no significant difference on the posttest. These results suggest that sex differences in embedded-figures scores found by many previous experimenters may have been associated with differences in prior experience in spatial skills and by a confounding of sex with area of academic study."}
{"id": "PMID:482056", "title": "Role of extended perceptual experience upon haptic perception of nonrepresentational shapes.", "content": "The influence of amount and type of exploratory activity upon 3 male and 5 female undergraduates' ability to detect the symmaterical or asymmetrical characteristics of individually presented plastic nonrepresentational shapes was examined. A decrease in scanning time and number of identification errors between the first and fourth days of testing indicates that extended perceptual experience plays a major role in the efficient pick-up and utilization of haptic information. A signal-detection analysis demonstrated that the use of a simultaneous-apprehension scanning strategy resulted in greater sensitivity to asymmetrical shapes than did the use of a simultaneous-apprehension scan. The data support the hypothesis that experience, the form parameter of symmetry, and the type of scanning strategy used to explore a stimulus function together to influence haptic perception of nonrepresentational shapes.", "contents": "Role of extended perceptual experience upon haptic perception of nonrepresentational shapes. The influence of amount and type of exploratory activity upon 3 male and 5 female undergraduates' ability to detect the symmaterical or asymmetrical characteristics of individually presented plastic nonrepresentational shapes was examined. A decrease in scanning time and number of identification errors between the first and fourth days of testing indicates that extended perceptual experience plays a major role in the efficient pick-up and utilization of haptic information. A signal-detection analysis demonstrated that the use of a simultaneous-apprehension scanning strategy resulted in greater sensitivity to asymmetrical shapes than did the use of a simultaneous-apprehension scan. The data support the hypothesis that experience, the form parameter of symmetry, and the type of scanning strategy used to explore a stimulus function together to influence haptic perception of nonrepresentational shapes."}
{"id": "PMID:482057", "title": "Perceived exertion of professional hockey players.", "content": "18 professional hockey players were tested on the treadmill with a modified Balke procedure and also for maximum isokinetic strength with the Cybex II instrument. Immediately after each test the subject completed the Borg Scale of Perceived Exertion. The treadmill effort was perceived as being \"somewhat hard work\" while the maximum strength effort was considered \"very light.\" This conflicts with previous literature. It may be that professional hockey players adapt to physical stress and do not respond perceptually to such stimuli in a typical manner.", "contents": "Perceived exertion of professional hockey players. 18 professional hockey players were tested on the treadmill with a modified Balke procedure and also for maximum isokinetic strength with the Cybex II instrument. Immediately after each test the subject completed the Borg Scale of Perceived Exertion. The treadmill effort was perceived as being \"somewhat hard work\" while the maximum strength effort was considered \"very light.\" This conflicts with previous literature. It may be that professional hockey players adapt to physical stress and do not respond perceptually to such stimuli in a typical manner."}
{"id": "PMID:482093", "title": "Studies on seminal vesiculitis in the bull. II. Malformation of the pelvic genital organs as a possible predisposing factor in the pathogenesis of seminal vesiculitis.", "content": "Of 32 bulls showing inflammatory conditions in the pelvic genital organs, 25 were examined post mortem (24 showing vesiculitis with or without ampullitis and one showing ampullitis alone). In ten cases (40%) the following malformations or rare anatomical deviations were demonstrated by careful gross-anatomical dissection: Segmental aplasia of the Wolffian duct: 1; segmental hypoplasia of the Wolffian duct: 3; asymmetric implantation of the two ampullae: 3; accessory vesicular glands: 1; uterus masculinus persistens: 2. These results corroborate earlier observations that a congenital defect may play a certain r\u00f4le in the pathogenesis of seminal vesiculitis, most probably by acting as a locus minoris resistentiae in or around colliculus seminalis. On account of the above findings, and considering the prognosis for the condition and the lack of effective treatment, it can in general be recommended, that diseased bulls should be slaughtered as soon as the diagnosis seminal vesiculitis is final.", "contents": "Studies on seminal vesiculitis in the bull. II. Malformation of the pelvic genital organs as a possible predisposing factor in the pathogenesis of seminal vesiculitis. Of 32 bulls showing inflammatory conditions in the pelvic genital organs, 25 were examined post mortem (24 showing vesiculitis with or without ampullitis and one showing ampullitis alone). In ten cases (40%) the following malformations or rare anatomical deviations were demonstrated by careful gross-anatomical dissection: Segmental aplasia of the Wolffian duct: 1; segmental hypoplasia of the Wolffian duct: 3; asymmetric implantation of the two ampullae: 3; accessory vesicular glands: 1; uterus masculinus persistens: 2. These results corroborate earlier observations that a congenital defect may play a certain r\u00f4le in the pathogenesis of seminal vesiculitis, most probably by acting as a locus minoris resistentiae in or around colliculus seminalis. On account of the above findings, and considering the prognosis for the condition and the lack of effective treatment, it can in general be recommended, that diseased bulls should be slaughtered as soon as the diagnosis seminal vesiculitis is final."}
{"id": "PMID:482094", "title": "Pyometra in the dog. III.--A pathophysiological investigation. III. Plasma progesterone levels and ovarian morphology.", "content": "The peripheral plasma progesterone levels in bitches with spontaneously developed pyometra did not differ significantly from those in normally cycling or pregnant bitches. The macroscopic ovarian morphology gave no indication of an increased corpus luteum activity. Ovarian follicular cysts were found in 4 out of 31 cases. There was no detectable difference of results between 14 bitches that had received hormonal medication 10 years to 1 week previous to the pyometra diagnosis, and the other patients.", "contents": "Pyometra in the dog. III.--A pathophysiological investigation. III. Plasma progesterone levels and ovarian morphology. The peripheral plasma progesterone levels in bitches with spontaneously developed pyometra did not differ significantly from those in normally cycling or pregnant bitches. The macroscopic ovarian morphology gave no indication of an increased corpus luteum activity. Ovarian follicular cysts were found in 4 out of 31 cases. There was no detectable difference of results between 14 bitches that had received hormonal medication 10 years to 1 week previous to the pyometra diagnosis, and the other patients."}
{"id": "PMID:482095", "title": "[Duovac 300--udder health and milking routines. A field experiment (author's transl)].", "content": "The effect on udder health and milking routine of two Alfa Laval milking units, the Duovac 300 with a normal (380 mm Hg) and a low (250 mm Hg) vacuum phase and the standard HP 100 unit with only the normal (380 mm Hg) vacuum phase, was investigated on six dairy farms. A total of 197 cows were involved in the study. Each herd was divided into two groups which were as similar as possible in terms of age, stage of parturition and milk yield. One group was milked with the Duovac unit, while the other was milked with the HP 100 units for a test period of 100 days. No differences in cell counts from the cow samples, the presence of subclinical mastitis or clinical condition of the teats were observed between the two test groups. The total milking machine time for the Duovac units was longer than for the HP 100 units. However, the time spent in the normal vacuum phase (380 mm Hg) for the Duovac units was shorter than the total milking machine time for the HP 100 units (380 mm Hg). The Duovac units had the shortest machine stripping time, but the \"waiting time\" for the milkers (the time between the various operations) was longer. These time studies showed that the number of cows milked per hour by one man using four or five Duovac units was equal to or greater than the number of cows milked per hour by one man using three HP 100 units. It was recommended that no more than three HP 100 units be managed by one man.", "contents": "[Duovac 300--udder health and milking routines. A field experiment (author's transl)]. The effect on udder health and milking routine of two Alfa Laval milking units, the Duovac 300 with a normal (380 mm Hg) and a low (250 mm Hg) vacuum phase and the standard HP 100 unit with only the normal (380 mm Hg) vacuum phase, was investigated on six dairy farms. A total of 197 cows were involved in the study. Each herd was divided into two groups which were as similar as possible in terms of age, stage of parturition and milk yield. One group was milked with the Duovac unit, while the other was milked with the HP 100 units for a test period of 100 days. No differences in cell counts from the cow samples, the presence of subclinical mastitis or clinical condition of the teats were observed between the two test groups. The total milking machine time for the Duovac units was longer than for the HP 100 units. However, the time spent in the normal vacuum phase (380 mm Hg) for the Duovac units was shorter than the total milking machine time for the HP 100 units (380 mm Hg). The Duovac units had the shortest machine stripping time, but the \"waiting time\" for the milkers (the time between the various operations) was longer. These time studies showed that the number of cows milked per hour by one man using four or five Duovac units was equal to or greater than the number of cows milked per hour by one man using three HP 100 units. It was recommended that no more than three HP 100 units be managed by one man."}
{"id": "PMID:482098", "title": "[Measurement of blood theophylline levels in the child: will this affect methods of prescription? (author's transl)].", "content": "Twelve children aged between 2 and 13 years received an oral dose of 5 mg/kg of pure theophylline, and 28 children aged between 2 and 15 years were given 10 mg/kg/day of the same drug in 3 or 4 divided doses for 3 days. In the first group blood theophylline levels were higher than 10 microgram/ml, after 1 or 3 hours, in only 3 children, two of whom were receiving erythromycin at the same time. Six hours and 12 hours later, none of the serum levels were higher than 10 microgram/ml. In the second group, estimations were performed on the 4th day. At 8 a.m., when the last dose had been given 12 hours previously, blood theophylline levels were all less than 10 microgram/ml (mean: 4.03 +/- 0,88 microgram/ml). Two hours and four hours after the usual morning dose, serum levels of greater than 10 microgram/ml were found in respectively 35 and 25% of the children only. Since the bronchodilator effect of theophylline is optimal for serum levels of greater than 10 microgram/ml, the dose currently recommended in France (10 mg/kg/day) would thus appear to be insufficient in most instances. However, increase in individual doses must be guided by serum estimations, which make it possible to avoid complications related to overdosage.", "contents": "[Measurement of blood theophylline levels in the child: will this affect methods of prescription? (author's transl)]. Twelve children aged between 2 and 13 years received an oral dose of 5 mg/kg of pure theophylline, and 28 children aged between 2 and 15 years were given 10 mg/kg/day of the same drug in 3 or 4 divided doses for 3 days. In the first group blood theophylline levels were higher than 10 microgram/ml, after 1 or 3 hours, in only 3 children, two of whom were receiving erythromycin at the same time. Six hours and 12 hours later, none of the serum levels were higher than 10 microgram/ml. In the second group, estimations were performed on the 4th day. At 8 a.m., when the last dose had been given 12 hours previously, blood theophylline levels were all less than 10 microgram/ml (mean: 4.03 +/- 0,88 microgram/ml). Two hours and four hours after the usual morning dose, serum levels of greater than 10 microgram/ml were found in respectively 35 and 25% of the children only. Since the bronchodilator effect of theophylline is optimal for serum levels of greater than 10 microgram/ml, the dose currently recommended in France (10 mg/kg/day) would thus appear to be insufficient in most instances. However, increase in individual doses must be guided by serum estimations, which make it possible to avoid complications related to overdosage."}
{"id": "PMID:482099", "title": "[Severe drug complications: current prevalence amongst patients admitted to adult intensive care units (author's transl)].", "content": "A retrospective study involving nine teaching hospital mixed intensive units provided information concerning severe drug complications amongst patients admitted to these departments over the past ten years. Amongst a total of 63717 patients admitted, there were 1132 drug complications (1.8%) without any predominance in terms of sex. Mortality was high (252 patients, i.e. 22.2%), being all the greater in older patients. Details concerning the various drug complications are given, the most common being anaphylactic shock and haemorrhage due to anticoagulants. These statistics are compared withthose already published by hospital departments of internal medicine.", "contents": "[Severe drug complications: current prevalence amongst patients admitted to adult intensive care units (author's transl)]. A retrospective study involving nine teaching hospital mixed intensive units provided information concerning severe drug complications amongst patients admitted to these departments over the past ten years. Amongst a total of 63717 patients admitted, there were 1132 drug complications (1.8%) without any predominance in terms of sex. Mortality was high (252 patients, i.e. 22.2%), being all the greater in older patients. Details concerning the various drug complications are given, the most common being anaphylactic shock and haemorrhage due to anticoagulants. These statistics are compared withthose already published by hospital departments of internal medicine."}
{"id": "PMID:482100", "title": "[Haemorrhagic complications using streptokinase during 98 treatments. Place of the biological surveillance (author's transl)].", "content": "Streptokinase was administered to 98 patients, 75 of them with arterial occlusion of the limbs, in accordance with the classical protocol of high and continuous doses (150,000 international units per hour for 48 to 78 hours after an initial standard dose of 500,000 units). There were a total of 15 haemorrhagic complications: --related to a non-respected contraindication (1 case) --traumatic (4 cases) --spontaneous (10 cases), responsible for or contributing to a fatal outcome in 4 instances. In addition, there were 13 spontaneous or provoked haemorrhagic side effects. Retrospective analysis of laboratory results shows that it is difficult to predict haemorrhage: the degree of fibrinopaenia and fibrin breackdown product levels are not closely related to the onset of bleeding. Nevertheless, the combination of a residual fibrin level of less than 1.50 g/l approximately with an amount of fibrinogen broken down (difference between fibrinogen levels before treatment and during treatment) of more than 3 grams was present in the great majority of patients in whom complications developed. The absence of bleeding seen when plasma thrombolytic activity, assessed by the Blix test, is inadequate, suggests the existence of a relationship between biological effectiveness and the risk of haemorrhage. In practice, apart from strict observation of contraindications, the risk of haemorrhage must be born in mind when the therapeutic decision is made. Careful clinical surveillance is today more important than laboratory studies in the prevention of haemorrhagic complications, the price which must be paid for the thrombolytic activity of streptokinase. The latter is capable of acting not only upon the vascular obstruction but also upon haemostatic clots.", "contents": "[Haemorrhagic complications using streptokinase during 98 treatments. Place of the biological surveillance (author's transl)]. Streptokinase was administered to 98 patients, 75 of them with arterial occlusion of the limbs, in accordance with the classical protocol of high and continuous doses (150,000 international units per hour for 48 to 78 hours after an initial standard dose of 500,000 units). There were a total of 15 haemorrhagic complications: --related to a non-respected contraindication (1 case) --traumatic (4 cases) --spontaneous (10 cases), responsible for or contributing to a fatal outcome in 4 instances. In addition, there were 13 spontaneous or provoked haemorrhagic side effects. Retrospective analysis of laboratory results shows that it is difficult to predict haemorrhage: the degree of fibrinopaenia and fibrin breackdown product levels are not closely related to the onset of bleeding. Nevertheless, the combination of a residual fibrin level of less than 1.50 g/l approximately with an amount of fibrinogen broken down (difference between fibrinogen levels before treatment and during treatment) of more than 3 grams was present in the great majority of patients in whom complications developed. The absence of bleeding seen when plasma thrombolytic activity, assessed by the Blix test, is inadequate, suggests the existence of a relationship between biological effectiveness and the risk of haemorrhage. In practice, apart from strict observation of contraindications, the risk of haemorrhage must be born in mind when the therapeutic decision is made. Careful clinical surveillance is today more important than laboratory studies in the prevention of haemorrhagic complications, the price which must be paid for the thrombolytic activity of streptokinase. The latter is capable of acting not only upon the vascular obstruction but also upon haemostatic clots."}
{"id": "PMID:482101", "title": "[Postoperative haemodynamic evaluation of myocardial protection by physicochemical cardioplegia. Hyperkaliemic, hyperosmolar and acid solution at 4 degrees C (author's transl)].", "content": "Myocardial protection by a combination of cardiac hypothermia and chemical cardioplegia (high concentration of K+ and Mg++, hyperosmolarity, acid pH), was studied during 20 valve replacements. Essentially assessed on the basis of repeated measurements of postoperative cardiac output, the results were compared with those obtained previously using local hypothermia only (20 patients) and hypothermic cardioplegia using Ringer Lactate (20 patients). Analysis of haemodynamic data, which thus involved 60 patients, indicated the superiority of physicochemical cardioplegia, and this for clamping periods of up to 2 hours, but also showed the desirability of changes in the protection protocol during the ischaemia phase and during reperfusion which might improve the results.", "contents": "[Postoperative haemodynamic evaluation of myocardial protection by physicochemical cardioplegia. Hyperkaliemic, hyperosmolar and acid solution at 4 degrees C (author's transl)]. Myocardial protection by a combination of cardiac hypothermia and chemical cardioplegia (high concentration of K+ and Mg++, hyperosmolarity, acid pH), was studied during 20 valve replacements. Essentially assessed on the basis of repeated measurements of postoperative cardiac output, the results were compared with those obtained previously using local hypothermia only (20 patients) and hypothermic cardioplegia using Ringer Lactate (20 patients). Analysis of haemodynamic data, which thus involved 60 patients, indicated the superiority of physicochemical cardioplegia, and this for clamping periods of up to 2 hours, but also showed the desirability of changes in the protection protocol during the ischaemia phase and during reperfusion which might improve the results."}
{"id": "PMID:482102", "title": "[Paraneoplastic hypercalcaemia in a case of carcinoma of the bladder. Demonstration in the tumour of a substance with parathormone activity (author's transl)].", "content": "Hypercalcaemia accompanied a bladder tumour in a 77-year-old woman. Immuno-reactive parathormone (IPTH) in the tumour being high, the hypercalcaemia could thus be attributed to secretion by the tumour of a substance with parathormone activity. The rarity of bladder tumours at the origin of paraneoplastic hypercalcaemia is recalled. The mechanism of the metabolic disturbance and the disagreement between serum and tumour levels of IPTH are discussed.", "contents": "[Paraneoplastic hypercalcaemia in a case of carcinoma of the bladder. Demonstration in the tumour of a substance with parathormone activity (author's transl)]. Hypercalcaemia accompanied a bladder tumour in a 77-year-old woman. Immuno-reactive parathormone (IPTH) in the tumour being high, the hypercalcaemia could thus be attributed to secretion by the tumour of a substance with parathormone activity. The rarity of bladder tumours at the origin of paraneoplastic hypercalcaemia is recalled. The mechanism of the metabolic disturbance and the disagreement between serum and tumour levels of IPTH are discussed."}
{"id": "PMID:482103", "title": "[Low postero-medial approach to the leg (author's transl)].", "content": "It is possible to prolong a postero-medial surgical approach to the leg as far as the tarsal canal in order to gain access to the posterioir aspect of the diaphysis and of the shaft of the tibia up to the tibio-tarsal line. The tendons and vessels must be displaced backwards, held by a tape and moved as necessary according to the desired approach. In addition, the morphology of the posterior aspect of the tibia is such that osteosynthesis is much simpler than via any other approach. Four years' experience of more than 30 cases enable the authors to advise the use of this approach in surgery of the lower end of the tibia.", "contents": "[Low postero-medial approach to the leg (author's transl)]. It is possible to prolong a postero-medial surgical approach to the leg as far as the tarsal canal in order to gain access to the posterioir aspect of the diaphysis and of the shaft of the tibia up to the tibio-tarsal line. The tendons and vessels must be displaced backwards, held by a tape and moved as necessary according to the desired approach. In addition, the morphology of the posterior aspect of the tibia is such that osteosynthesis is much simpler than via any other approach. Four years' experience of more than 30 cases enable the authors to advise the use of this approach in surgery of the lower end of the tibia."}
{"id": "PMID:482111", "title": "[Cold nodules of the thyroid. Reevaluation of surgical excision on the basis of a new group of 607 patients(author's transl)].", "content": "In a serie of 607 cold nodules developing in normal or apparently normal (on preoperative clinical examination) thyroids, the results of histopathological examination were compared with those in an identical series studied 10 years before. Carcinoma was frequent: 9.7% in the current series and 19% in the first series. Insofar as neither clinical examination nor special investigations at the present time make it possible to affirm that a cold nodule is not malignant, surgical complete lobectomy with frozen section examination remains the required procedure in view of its harmless nature and the good prognosis in differentiated tumours operated upon before any visceral extension has taken place, contrasting with the poor prognosis of others. Recurrences may be prevented by special operative techniques (routine excision of Lalouette's pyramid, division of the contralateral ligament of Grubert) and routine postoperative thyroid extracts in patients with a morphological, familial or biological risk of recurrence.", "contents": "[Cold nodules of the thyroid. Reevaluation of surgical excision on the basis of a new group of 607 patients(author's transl)]. In a serie of 607 cold nodules developing in normal or apparently normal (on preoperative clinical examination) thyroids, the results of histopathological examination were compared with those in an identical series studied 10 years before. Carcinoma was frequent: 9.7% in the current series and 19% in the first series. Insofar as neither clinical examination nor special investigations at the present time make it possible to affirm that a cold nodule is not malignant, surgical complete lobectomy with frozen section examination remains the required procedure in view of its harmless nature and the good prognosis in differentiated tumours operated upon before any visceral extension has taken place, contrasting with the poor prognosis of others. Recurrences may be prevented by special operative techniques (routine excision of Lalouette's pyramid, division of the contralateral ligament of Grubert) and routine postoperative thyroid extracts in patients with a morphological, familial or biological risk of recurrence."}
{"id": "PMID:482112", "title": "[Prolonged treatment of Zollinger-Ellison by cimetidine (author's transl)].", "content": "Treatment of Zollinger-Ellison syndrome with cimetidine is justified by prior pharmacological studies; close survey of the patients is necessary, using clinical (endoscopic control of ulcer; diarrhea) and biological (BOA measurements and assessment of cimetidine efficiency) criteria. On the basis of 7 patients suffering from of chronic forms of ZES treated with cimetidine for periods varying from 56 to 570 days, a therapeutic scheme is suggested, allowing individual choices in the following problems:-possibility of gastrinoma complete excision, -advantages and disadvantages of cimetidine vs. total gastrectomy in respect to side-effects and acid secretion suppression, -variations in drug efficacy according to time (prolonged secretory inhibition, escape phenomenon).", "contents": "[Prolonged treatment of Zollinger-Ellison by cimetidine (author's transl)]. Treatment of Zollinger-Ellison syndrome with cimetidine is justified by prior pharmacological studies; close survey of the patients is necessary, using clinical (endoscopic control of ulcer; diarrhea) and biological (BOA measurements and assessment of cimetidine efficiency) criteria. On the basis of 7 patients suffering from of chronic forms of ZES treated with cimetidine for periods varying from 56 to 570 days, a therapeutic scheme is suggested, allowing individual choices in the following problems:-possibility of gastrinoma complete excision, -advantages and disadvantages of cimetidine vs. total gastrectomy in respect to side-effects and acid secretion suppression, -variations in drug efficacy according to time (prolonged secretory inhibition, escape phenomenon)."}
{"id": "PMID:482113", "title": "[Arthroscopic study after failure of synoviorthesis (author's transl)].", "content": "Arthroscopy of the knee, including synovial biopsy under direct vision, was performed in 22 patients (21 cases of arthritis, 1 of osteoarthrosis), treated 15 days to 12 months previously by osmic or isotopic synoviorthesis. The aspects seen after the 16 failures were compared with those seen after good results (4 cases) and after recurrences (2 cases). In arthritis, failure was due either to persistent synovitis, to synovial necrosis or to a combination of the two. Arthroscopy is useful since the discovery of inflammatory lessions justifies repeat synoviorthesis, whilst necrosis indicates the need for articular lavage.", "contents": "[Arthroscopic study after failure of synoviorthesis (author's transl)]. Arthroscopy of the knee, including synovial biopsy under direct vision, was performed in 22 patients (21 cases of arthritis, 1 of osteoarthrosis), treated 15 days to 12 months previously by osmic or isotopic synoviorthesis. The aspects seen after the 16 failures were compared with those seen after good results (4 cases) and after recurrences (2 cases). In arthritis, failure was due either to persistent synovitis, to synovial necrosis or to a combination of the two. Arthroscopy is useful since the discovery of inflammatory lessions justifies repeat synoviorthesis, whilst necrosis indicates the need for articular lavage."}
{"id": "PMID:482114", "title": "[Steiner-type neuroapudomatosis presented a pseudo-Hirschsprung disease (author's transl)].", "content": "A case of Steiner type neuroapudomatosis presented with a pseudo-Hirschsprung disease in an infant. Secondarily, the facies became typical, while hyperthyrocalcitoninaemia led to the discovery at the age of 2 years and 8 months of a medullary carcinoma of the thyroid. Despite early surgery in the absence of any clinical signs, it was not possible to avoid cervical lymphatic spread.", "contents": "[Steiner-type neuroapudomatosis presented a pseudo-Hirschsprung disease (author's transl)]. A case of Steiner type neuroapudomatosis presented with a pseudo-Hirschsprung disease in an infant. Secondarily, the facies became typical, while hyperthyrocalcitoninaemia led to the discovery at the age of 2 years and 8 months of a medullary carcinoma of the thyroid. Despite early surgery in the absence of any clinical signs, it was not possible to avoid cervical lymphatic spread."}
{"id": "PMID:482127", "title": "Ethidium bromide binding to core particle: comparison with native chromatin.", "content": "Ethidium bromide intercalation into DNA of nuclease digested erythrocyte chromatin and core particle, was followed at low ionic strength by fluorescence measurements, equilibrium dialysis using 14C labelled dye, circular dichroism and electron microscopy. High affinity binding sites in the chromatin are no more present in the core particle, i.e. when the linker is removed. In the case of core particle, a cooperative process occurs, accompanied by a partial stripping of the DNA from the core histone. Finally two populations of core particles can be detected by electron microscopy as far as their binding properties are concerned.", "contents": "Ethidium bromide binding to core particle: comparison with native chromatin. Ethidium bromide intercalation into DNA of nuclease digested erythrocyte chromatin and core particle, was followed at low ionic strength by fluorescence measurements, equilibrium dialysis using 14C labelled dye, circular dichroism and electron microscopy. High affinity binding sites in the chromatin are no more present in the core particle, i.e. when the linker is removed. In the case of core particle, a cooperative process occurs, accompanied by a partial stripping of the DNA from the core histone. Finally two populations of core particles can be detected by electron microscopy as far as their binding properties are concerned."}
{"id": "PMID:482128", "title": "Cycle specific association of nascent chromatin with nuclear envelope components in Physarum polycephalum.", "content": "The action of heparin on isolated nuclei derived from different phases of the mitotic cycle in plasmodia of Physarum polycephalum was studied. Heparin addition at two-fold excess over DNA concentration to nuclei in Mg-free low ionic strength buffer (10 mM TRIS-HC1, 10 mM Na2 HPO4, pH = 8) releases 60-80% of chromatin from S, G2, and mitotic phase nuclei. The RNA/protein ratio of herparin-solubilized cromatin is constant through S and G2 phases, but rises about two-fold at early prophase coincident with nucleolar breakdown. Purified nuclear envelopes were obtained from heparin-treated nuclei by sedimentation according to Bornens procedures (Nature 244, 28, 1973), and examined by transmission electron microscopy. Residual chromatin is seen at all stages with fine network of DNA fibrils in contact with the envelop. Regardless of time in S, 80% of 3H-labeled DNA was released into soluble chromatin with identical 3H/14C ratios. The residual chromatin in nuclear envelopes exhibited a preferential association of early S-DNA in nuclei engaged in early S replication, and late S preferential association in nuclei engaged in late S replication.", "contents": "Cycle specific association of nascent chromatin with nuclear envelope components in Physarum polycephalum. The action of heparin on isolated nuclei derived from different phases of the mitotic cycle in plasmodia of Physarum polycephalum was studied. Heparin addition at two-fold excess over DNA concentration to nuclei in Mg-free low ionic strength buffer (10 mM TRIS-HC1, 10 mM Na2 HPO4, pH = 8) releases 60-80% of chromatin from S, G2, and mitotic phase nuclei. The RNA/protein ratio of herparin-solubilized cromatin is constant through S and G2 phases, but rises about two-fold at early prophase coincident with nucleolar breakdown. Purified nuclear envelopes were obtained from heparin-treated nuclei by sedimentation according to Bornens procedures (Nature 244, 28, 1973), and examined by transmission electron microscopy. Residual chromatin is seen at all stages with fine network of DNA fibrils in contact with the envelop. Regardless of time in S, 80% of 3H-labeled DNA was released into soluble chromatin with identical 3H/14C ratios. The residual chromatin in nuclear envelopes exhibited a preferential association of early S-DNA in nuclei engaged in early S replication, and late S preferential association in nuclei engaged in late S replication."}
{"id": "PMID:482129", "title": "Further studies on partially purified calf thymus DNA polymerase a.", "content": "Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit.", "contents": "Further studies on partially purified calf thymus DNA polymerase a. Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit."}
{"id": "PMID:482130", "title": "Modulation of thyroglobulin messenger RNA level by thyrotropin in cultured thyroid cells.", "content": "To examine the influence of thyrotropin (TSH) on the thyroglobulin (Tgb) mRNA content, the latter was evaluated in the cytoplasm of hog thyroid cells cultured in the absence (control cells) or presence of TSH. The Tgb mRNA levels were determined by, (i) kinetics of hybridization to sheep Tgb cDNA, (ii) capacity of coding for peptides immunologically related to Tgb in reticulocyte lysate. In cells cultured for 4 days in the absence of TSH, the content of Tgb mRNA sequences decreased to 30% of its initial value and the messenger activity to 15%. Conversely, TSH maintained the initial Tgb mRNA level in cells cultured in its presence, and TSH concentrations 50 micronU/ml or 5 mU/ml gave identical results. At each period tested poly (A) content was the same in TSH-treated and control cells. When TSH was added to media after 4 or 8 days culture without TSH, the Tgb mRNA level was partially restored. These results suggest that TSH exerts a positive control on Tgb gene expression through modulation of Tgb mRNA content of thyroid cells.", "contents": "Modulation of thyroglobulin messenger RNA level by thyrotropin in cultured thyroid cells. To examine the influence of thyrotropin (TSH) on the thyroglobulin (Tgb) mRNA content, the latter was evaluated in the cytoplasm of hog thyroid cells cultured in the absence (control cells) or presence of TSH. The Tgb mRNA levels were determined by, (i) kinetics of hybridization to sheep Tgb cDNA, (ii) capacity of coding for peptides immunologically related to Tgb in reticulocyte lysate. In cells cultured for 4 days in the absence of TSH, the content of Tgb mRNA sequences decreased to 30% of its initial value and the messenger activity to 15%. Conversely, TSH maintained the initial Tgb mRNA level in cells cultured in its presence, and TSH concentrations 50 micronU/ml or 5 mU/ml gave identical results. At each period tested poly (A) content was the same in TSH-treated and control cells. When TSH was added to media after 4 or 8 days culture without TSH, the Tgb mRNA level was partially restored. These results suggest that TSH exerts a positive control on Tgb gene expression through modulation of Tgb mRNA content of thyroid cells."}
{"id": "PMID:482131", "title": "The effect of aphidicolin on adenovirus DNA synthesis.", "content": "Aphidicolin inhibits adenovirus DNA replication in HeLa cells and in a cell-free, infected, nuclear extract in which viral DNA is elongated. The compound inhibits alpha DNA polymerase, extensively purified from HeLa cells, but has little or no effect on the beta or gamma DNA polymerases similarly purified. Aphidicolin does not affect thymidine uptake by cells nor does synthesis as it also inhibits DNA replication in uninfected cells. The inhibition by aphidicolin is reversible if the drug is removed within 18 hrs after addition to HeLa or Chinese Hamster Ovary cells but the cells are irreversibly affected if the drug remains for 48 hours.", "contents": "The effect of aphidicolin on adenovirus DNA synthesis. Aphidicolin inhibits adenovirus DNA replication in HeLa cells and in a cell-free, infected, nuclear extract in which viral DNA is elongated. The compound inhibits alpha DNA polymerase, extensively purified from HeLa cells, but has little or no effect on the beta or gamma DNA polymerases similarly purified. Aphidicolin does not affect thymidine uptake by cells nor does synthesis as it also inhibits DNA replication in uninfected cells. The inhibition by aphidicolin is reversible if the drug is removed within 18 hrs after addition to HeLa or Chinese Hamster Ovary cells but the cells are irreversibly affected if the drug remains for 48 hours."}
{"id": "PMID:482132", "title": "DNA-binding activity of tightly-bound nonhistone chromosomal proteins in chicken liver chromatin.", "content": "We have isolated a nonhistone chromosomal protein fraction from chicken liver chromatin which possesses high affinity and preferential sequence DNA binding. Residually DNA-bound nonhistone chromosomal proteins after 2.0 M NaCl extraction of bulk chromatin are isolated. Bound proteins are released by dissociation of the complexes in 5.0 M urea/3.0 M NaCl. We have investigated the in vitro DNA-binding properties of this class. In contrast to other DNA-binding NHCP whose activities have been studied, direct DNA-binding activity is observed which is not abolished under conditions of high ionic strength (to 3.0 M NaCl). Strong preference in binding fractionated homologous DNA is observed, while binding of heterologous (E. Coli) DNA is negligible. The fractionation of homologous DNA permits the isolation of DNA for which this protein class displays strong binding preference, presumably through a concentration of binding sites. The composite data suggest sequence-specific interaction between this protein class and DNA, which is not abolished by high ionic strength.", "contents": "DNA-binding activity of tightly-bound nonhistone chromosomal proteins in chicken liver chromatin. We have isolated a nonhistone chromosomal protein fraction from chicken liver chromatin which possesses high affinity and preferential sequence DNA binding. Residually DNA-bound nonhistone chromosomal proteins after 2.0 M NaCl extraction of bulk chromatin are isolated. Bound proteins are released by dissociation of the complexes in 5.0 M urea/3.0 M NaCl. We have investigated the in vitro DNA-binding properties of this class. In contrast to other DNA-binding NHCP whose activities have been studied, direct DNA-binding activity is observed which is not abolished under conditions of high ionic strength (to 3.0 M NaCl). Strong preference in binding fractionated homologous DNA is observed, while binding of heterologous (E. Coli) DNA is negligible. The fractionation of homologous DNA permits the isolation of DNA for which this protein class displays strong binding preference, presumably through a concentration of binding sites. The composite data suggest sequence-specific interaction between this protein class and DNA, which is not abolished by high ionic strength."}
{"id": "PMID:482162", "title": "Studies on the cause of changes of reactivity to hexobarbital in spontaneously hypertensive rats (SHR).", "content": "The hypnotic action of hexobarbital is weaker in spontaneously hypertensive rats (SHR) than in Wistar rats. The difference does not appear in young rats (up to the sixth week of life) and appears gradually concomitantly with maturation of animals. There was no correlation between the changes of reactivity to hexobarbital and development of hypertension. The rate of metabolism of hexobarbital was similar in SHR and Wistar rats. There is a close relationship between the changes in reactivity to hexobarbital and psychomotor activity: both are significantly higher in SHR than in Wistar rats. The results suggest that the main cause of a weaker action of hexobarbital in SHR is higher intensity of stimulatory processes in the central nervous system.", "contents": "Studies on the cause of changes of reactivity to hexobarbital in spontaneously hypertensive rats (SHR). The hypnotic action of hexobarbital is weaker in spontaneously hypertensive rats (SHR) than in Wistar rats. The difference does not appear in young rats (up to the sixth week of life) and appears gradually concomitantly with maturation of animals. There was no correlation between the changes of reactivity to hexobarbital and development of hypertension. The rate of metabolism of hexobarbital was similar in SHR and Wistar rats. There is a close relationship between the changes in reactivity to hexobarbital and psychomotor activity: both are significantly higher in SHR than in Wistar rats. The results suggest that the main cause of a weaker action of hexobarbital in SHR is higher intensity of stimulatory processes in the central nervous system."}
{"id": "PMID:482163", "title": "The effect of pyrogen induced fever on pharmacokinetics of rifamycin SV.", "content": "The fever was evoked in rabbits by single iv injection of pyrogen-standard from E. coli, and rifamycin SV was administered at the peak of the fever (T = 1.7 degrees C). The rifamycin blood level was determined during the fever stability and changes of kinetics of the antibiotic were investigated. It was found that distribution and elimination of rifamycin were decreased during fever. The consequence of the inhibition of the antibiotic elimination was the increase of its level in blood and tissue compartments.", "contents": "The effect of pyrogen induced fever on pharmacokinetics of rifamycin SV. The fever was evoked in rabbits by single iv injection of pyrogen-standard from E. coli, and rifamycin SV was administered at the peak of the fever (T = 1.7 degrees C). The rifamycin blood level was determined during the fever stability and changes of kinetics of the antibiotic were investigated. It was found that distribution and elimination of rifamycin were decreased during fever. The consequence of the inhibition of the antibiotic elimination was the increase of its level in blood and tissue compartments."}
{"id": "PMID:482169", "title": "Kidney stone disease: evaluation and medical management.", "content": "Over the past 15 years we have developed a protocol for evaluation and medical management of urinary calculi that has proved quite effective. For all patients we include plain x-ray films of the urinary tract, intravenous pyelograms, urine cultures and sensitivity studies, serum and 24-hour urinary chemical determinations, and analysis of any stones recovered. Although the cause of calcium stones, the most common type of urinary calculi, has not been established, specific treatment regimens have been devised. Maintenance of a dilute urine remains the mainstay of therapy, and diuretics, alkalinizing or acidifying agents, and various specific drugs may be indicated, depending on laboratory findings. Surgery is not indicated unless the stone is large, is in the upper part of the urinary tract, or shows no signs of movement.", "contents": "Kidney stone disease: evaluation and medical management. Over the past 15 years we have developed a protocol for evaluation and medical management of urinary calculi that has proved quite effective. For all patients we include plain x-ray films of the urinary tract, intravenous pyelograms, urine cultures and sensitivity studies, serum and 24-hour urinary chemical determinations, and analysis of any stones recovered. Although the cause of calcium stones, the most common type of urinary calculi, has not been established, specific treatment regimens have been devised. Maintenance of a dilute urine remains the mainstay of therapy, and diuretics, alkalinizing or acidifying agents, and various specific drugs may be indicated, depending on laboratory findings. Surgery is not indicated unless the stone is large, is in the upper part of the urinary tract, or shows no signs of movement."}
{"id": "PMID:482165", "title": "Sequiterpene lactones. Part XXV. Studies on the mode of action. The cellular and molecular basis of cytostatic action.", "content": "Two compounds, derivatives of the sesquiterpene lactones (SL), ALA and URSA, on the basis of the high antitumor activity in vitro and in vivo systems, were selected as models, in order to study the mechanism of action of this drug class. Using cytologic, morphologic and isotopic methods the following were demonstrated: 1) At a subtoxic conc., 0.28 microM, arrest the HeLa cell in its interphase (G1, and/or S, G2). 2) At a higher conc. of 28.0 microM, complete and irreversible cytotoxic effects with pyknosis and karyorrhexis. 3) At a conc. of 56.0 microM, inhibition of protein biosynthesis by 94% and RNA by 81--92%. 4) At a conc. of 2.5 microM inhibition by 80% of translation processes. Inhibition of DNA biosynthesis seems to be doubtful. A preliminary hypothetical model of the cellular and molecular mechanism of SL, partly explanatory also of the cytostatic effect, has been proposed.", "contents": "Sequiterpene lactones. Part XXV. Studies on the mode of action. The cellular and molecular basis of cytostatic action. Two compounds, derivatives of the sesquiterpene lactones (SL), ALA and URSA, on the basis of the high antitumor activity in vitro and in vivo systems, were selected as models, in order to study the mechanism of action of this drug class. Using cytologic, morphologic and isotopic methods the following were demonstrated: 1) At a subtoxic conc., 0.28 microM, arrest the HeLa cell in its interphase (G1, and/or S, G2). 2) At a higher conc. of 28.0 microM, complete and irreversible cytotoxic effects with pyknosis and karyorrhexis. 3) At a conc. of 56.0 microM, inhibition of protein biosynthesis by 94% and RNA by 81--92%. 4) At a conc. of 2.5 microM inhibition by 80% of translation processes. Inhibition of DNA biosynthesis seems to be doubtful. A preliminary hypothetical model of the cellular and molecular mechanism of SL, partly explanatory also of the cytostatic effect, has been proposed."}
{"id": "PMID:482171", "title": "Urinary tract infection in children: diagnosis and treatment.", "content": "Urinary tract infection (UTI) is a fairly frequent occurrence in children. Diagnosis should be made quickly and accurately by Gram staining and culture of an uncontaminated urine specimen. A first, uncomplicated UTI should be treated with sulfonamides or nitrofurantoin. Careful follow-up is necessary because of the high risk of recurrence. Sensitivity studies and specific antimicrobial therapy are indicated in any patient with recurrence and in patients with anatomic urinary tract abnormalities. Long-term prophylaxis may be needed.", "contents": "Urinary tract infection in children: diagnosis and treatment. Urinary tract infection (UTI) is a fairly frequent occurrence in children. Diagnosis should be made quickly and accurately by Gram staining and culture of an uncontaminated urine specimen. A first, uncomplicated UTI should be treated with sulfonamides or nitrofurantoin. Careful follow-up is necessary because of the high risk of recurrence. Sensitivity studies and specific antimicrobial therapy are indicated in any patient with recurrence and in patients with anatomic urinary tract abnormalities. Long-term prophylaxis may be needed."}
{"id": "PMID:482166", "title": "Hydrolysis derivatives of (pyridyl-2)acetonitrile.", "content": "The alkaline hydrolysis (KOH/EtOH) of alkyl derivatives of (pyridyl-2)acetonitriles gave the corresponding amides. Lactones: 3-(pyridyl-2')3-alkyl-5-methyl-tetrahydrofuran-2-ones were synthesized as result of the hydrolysis of (pyridyl-2) alkylallylacetamides in presence of 85% H3P04. Soporific and anticonvulsive activity exhibited (pyridyl-2)diallylacetic amide 2 and (pyridyl-2)di-n-propylacetamine 3.", "contents": "Hydrolysis derivatives of (pyridyl-2)acetonitrile. The alkaline hydrolysis (KOH/EtOH) of alkyl derivatives of (pyridyl-2)acetonitriles gave the corresponding amides. Lactones: 3-(pyridyl-2')3-alkyl-5-methyl-tetrahydrofuran-2-ones were synthesized as result of the hydrolysis of (pyridyl-2) alkylallylacetamides in presence of 85% H3P04. Soporific and anticonvulsive activity exhibited (pyridyl-2)diallylacetic amide 2 and (pyridyl-2)di-n-propylacetamine 3."}
{"id": "PMID:482172", "title": "Preinvasive cervical cancer: how it develops and how to block its progression.", "content": "The preinvasive stage of cervical cancer is usually so prolonged and the techniques for its diagnosis and treatment are so well advanced that aggressive surgical treatment should rarely be necessary and the disease should rarely be fatal. That these results have not yet been realized is due largely to the failure of screening programs to reach at-risk populations. At the same time, the widespread use of cytologic screening in some populations has revealed a large number of women with atypical findings who require evaluation and treatment. It is an ongoing clinical concern to protect these patients from the development of invasive disease without overtreating foci of disease that can be destroyed by simple techniques.", "contents": "Preinvasive cervical cancer: how it develops and how to block its progression. The preinvasive stage of cervical cancer is usually so prolonged and the techniques for its diagnosis and treatment are so well advanced that aggressive surgical treatment should rarely be necessary and the disease should rarely be fatal. That these results have not yet been realized is due largely to the failure of screening programs to reach at-risk populations. At the same time, the widespread use of cytologic screening in some populations has revealed a large number of women with atypical findings who require evaluation and treatment. It is an ongoing clinical concern to protect these patients from the development of invasive disease without overtreating foci of disease that can be destroyed by simple techniques."}
{"id": "PMID:482167", "title": "New derivatives of hexahydroazepine, hexahydro(1,4,5)oxadiazepine and hexahydro(1,4,5)oxadiazocine with expected pharmacological activity.", "content": "New derivatives with the expected pharmacological activity of heterocyclic systems mentioned above having ether (1-6) and ester (7-10) groups were obtained.", "contents": "New derivatives of hexahydroazepine, hexahydro(1,4,5)oxadiazepine and hexahydro(1,4,5)oxadiazocine with expected pharmacological activity. New derivatives with the expected pharmacological activity of heterocyclic systems mentioned above having ether (1-6) and ester (7-10) groups were obtained."}
{"id": "PMID:482173", "title": "Manifestations of gallstone disease.", "content": "In some patients, gallstones are asymptomatic, lying dormant in the gallbladder or wedged in the cystic duct. In others, stones cause specific symptoms of gallbladder disease, such as biliary colic, acute cholecystitis, or cholangitis. Symptoms of flatulent dyspepsia are not markers of gallstone disease, since they occur equally in those with and without gallstones. Complications of gallstone disease include pancreatitis, biliary-enteric fistulas, hydrops, limy bile, porcelain gallsbladder, and carcinoma of the gallbladder. Cholecystectomy is indicated for symptomatic gallstones; for suspected stones in diabetics, who are at high risk should complications of gallstone disease occur; and in a few other limited situations. Prophylactic cholecystectomy for asymptomatic gallstones remains controversial.", "contents": "Manifestations of gallstone disease. In some patients, gallstones are asymptomatic, lying dormant in the gallbladder or wedged in the cystic duct. In others, stones cause specific symptoms of gallbladder disease, such as biliary colic, acute cholecystitis, or cholangitis. Symptoms of flatulent dyspepsia are not markers of gallstone disease, since they occur equally in those with and without gallstones. Complications of gallstone disease include pancreatitis, biliary-enteric fistulas, hydrops, limy bile, porcelain gallsbladder, and carcinoma of the gallbladder. Cholecystectomy is indicated for symptomatic gallstones; for suspected stones in diabetics, who are at high risk should complications of gallstone disease occur; and in a few other limited situations. Prophylactic cholecystectomy for asymptomatic gallstones remains controversial."}
{"id": "PMID:482168", "title": "Effect of some peptides on distribution of rifamycin SV in rabbits.", "content": "The effect of insulin, glutathione, trypsin and somatotropin on processes of distribution and elimination of rifamycin SV were investigated using pharmacokinetic analysis. It was found that the peptides, especially glutathione and trypsin, enhance the distribution of rifamycin in rabbits and cause an increase of amount of this antibiotic in the central compartment.", "contents": "Effect of some peptides on distribution of rifamycin SV in rabbits. The effect of insulin, glutathione, trypsin and somatotropin on processes of distribution and elimination of rifamycin SV were investigated using pharmacokinetic analysis. It was found that the peptides, especially glutathione and trypsin, enhance the distribution of rifamycin in rabbits and cause an increase of amount of this antibiotic in the central compartment."}
{"id": "PMID:482174", "title": "Autoimmune hemolytic anemia. 3. Cold antibody type.", "content": "In cold autoimmune hemolytic anemia, the causative autoantibodies (cold agglutinins) are most reactive at low temperatures. The anti-I type of cold agglutinin is found in cold hemagglutination disease and in hemolytic anemia secondary to malignant lymphoma, leukemia, or atypical pneumonia. The anti-I type of cold agglutinin is found in many patients with infectious mononucleosis and in an occasional patient with other lymphoproliferative disorders. Paroxysmal cold hemoglobinuria, a disorder in which exposure to cold temperatures triggers massive hemolysis, is characterized by a unique biphasic cold autoantibody called the Donath-Landsteiner antibody. By way of summary and review of this and the two previous articles in this series, table 4 presents a practical approach to diagnosis of the autoimmune hemolytic anemias.", "contents": "Autoimmune hemolytic anemia. 3. Cold antibody type. In cold autoimmune hemolytic anemia, the causative autoantibodies (cold agglutinins) are most reactive at low temperatures. The anti-I type of cold agglutinin is found in cold hemagglutination disease and in hemolytic anemia secondary to malignant lymphoma, leukemia, or atypical pneumonia. The anti-I type of cold agglutinin is found in many patients with infectious mononucleosis and in an occasional patient with other lymphoproliferative disorders. Paroxysmal cold hemoglobinuria, a disorder in which exposure to cold temperatures triggers massive hemolysis, is characterized by a unique biphasic cold autoantibody called the Donath-Landsteiner antibody. By way of summary and review of this and the two previous articles in this series, table 4 presents a practical approach to diagnosis of the autoimmune hemolytic anemias."}
{"id": "PMID:482164", "title": "On the central antiserotonin action of trazodone.", "content": "Trazodone, an antidepressant drug with an unknown mechanism of action, has been examined in order to demonstrate its central antiserotonin action. Trazodone antagonizes the head twitch response induced by 5-hydroxytryptophan in rats and mice, or by-5-methoxytryptamine in rats (the ED50 values are 9.3, 5.2, and 10.8 mg/kg respectively). It counteracts convulsions induced by tryptamine in rats (ED50=3.75 mg/kg). Trazodone abolishes hyperthermia induced by serotoninomimetics (LSD, quipazine, fenfluramine) in rabbits. It does not affect ptosis induced by reserpine, and diminishes stimulation of the locomotor activity induced by amphetamine. Our findings demonstrate that trazodone has a central antiserotonin action, similarly as three other antidepressant drugs: mianserin, danitracen and doxepin, whose central antiserotonin action has been found previously.", "contents": "On the central antiserotonin action of trazodone. Trazodone, an antidepressant drug with an unknown mechanism of action, has been examined in order to demonstrate its central antiserotonin action. Trazodone antagonizes the head twitch response induced by 5-hydroxytryptophan in rats and mice, or by-5-methoxytryptamine in rats (the ED50 values are 9.3, 5.2, and 10.8 mg/kg respectively). It counteracts convulsions induced by tryptamine in rats (ED50=3.75 mg/kg). Trazodone abolishes hyperthermia induced by serotoninomimetics (LSD, quipazine, fenfluramine) in rabbits. It does not affect ptosis induced by reserpine, and diminishes stimulation of the locomotor activity induced by amphetamine. Our findings demonstrate that trazodone has a central antiserotonin action, similarly as three other antidepressant drugs: mianserin, danitracen and doxepin, whose central antiserotonin action has been found previously."}
{"id": "PMID:482176", "title": "Diagnostic/genetic studies in mental retardation.", "content": "Excluding environmental, psychotic, and unclassified categories, a total of 1,231 cases of severe mental retardation were found in the Central Wisconsin Center study. Some 45% of cases were estimated to be genetically caused or predisposed, but in only 15% was the risk of recurrence considered high. Nevertheless, I feel strongly that parents of all retarded patients deserve etiologic/diagnostic counseling.", "contents": "Diagnostic/genetic studies in mental retardation. Excluding environmental, psychotic, and unclassified categories, a total of 1,231 cases of severe mental retardation were found in the Central Wisconsin Center study. Some 45% of cases were estimated to be genetically caused or predisposed, but in only 15% was the risk of recurrence considered high. Nevertheless, I feel strongly that parents of all retarded patients deserve etiologic/diagnostic counseling."}
{"id": "PMID:482177", "title": "Thyrotoxicosis as a mimic of dementia and/or stroke-like syndrome.", "content": "The case reported here and others like it indicate that thyrotoxicosis can be manifested by psychosis or bizarre neurologic signs. Thus, hyperthyroidism in late life may be overlooked and the patient given the diagnosis of psychosis secondary to organic brain syndrome or cerebrovascular accident. Thyrotoxicosis, which is treatable, should be looked for as a possible cause of deterioration of mental function with or without focal neurologic signs.", "contents": "Thyrotoxicosis as a mimic of dementia and/or stroke-like syndrome. The case reported here and others like it indicate that thyrotoxicosis can be manifested by psychosis or bizarre neurologic signs. Thus, hyperthyroidism in late life may be overlooked and the patient given the diagnosis of psychosis secondary to organic brain syndrome or cerebrovascular accident. Thyrotoxicosis, which is treatable, should be looked for as a possible cause of deterioration of mental function with or without focal neurologic signs."}
{"id": "PMID:482178", "title": "Differential diagnosis of hypercalcemia: a mechanistic approach.", "content": "Malignant disease and primary hyperparathyroidism are the most common causes of hypercalcemia, but there are many minor causes. Mechanical or humoral factors, or both, may underlie the increase in bone resorption. Parathyroid hormone (PTH) is a major mediator of bone resorption, but many other humoral agents have the same effect, eg, prostaglandin, osteoclast-activating factor, and thyroid hormone. Serial determination of total calcium concentration is the most important laboratory test in hypercalcemia. Other useful tests include the determination of serum and urinary phosphorus concentration, chloride/phosphate ratio, urinary cyclic adenosine 3',5'-monophosphate (cAMP) level; carboxyl-terminal PTH assay; corticosteroid challenge; and appropriate radiologic studies. Nephrogenous cAMP and urinary prostaglandin determinations are research tools that hold great promise in the future. Differentiation between PTH- and non-PTH-mediated hypercalcemia determines subsequent steps in diagnosis and treatment.", "contents": "Differential diagnosis of hypercalcemia: a mechanistic approach. Malignant disease and primary hyperparathyroidism are the most common causes of hypercalcemia, but there are many minor causes. Mechanical or humoral factors, or both, may underlie the increase in bone resorption. Parathyroid hormone (PTH) is a major mediator of bone resorption, but many other humoral agents have the same effect, eg, prostaglandin, osteoclast-activating factor, and thyroid hormone. Serial determination of total calcium concentration is the most important laboratory test in hypercalcemia. Other useful tests include the determination of serum and urinary phosphorus concentration, chloride/phosphate ratio, urinary cyclic adenosine 3',5'-monophosphate (cAMP) level; carboxyl-terminal PTH assay; corticosteroid challenge; and appropriate radiologic studies. Nephrogenous cAMP and urinary prostaglandin determinations are research tools that hold great promise in the future. Differentiation between PTH- and non-PTH-mediated hypercalcemia determines subsequent steps in diagnosis and treatment."}
{"id": "PMID:482179", "title": "Causes of hypercalcemia.", "content": "Hypercalcemia is most commonly seen in normal infants as the result of normal rapid bone growth. The most common causative diseases are malignant disease and hyperparathyroidism. A variety of pharmacologic agents, especially vitamin D and its metabolites and thiazide diuretics, can elevate serum calcium levels. Hypersensitivity to vitamin D appears to be a cause of hypercalcemia in infants and in patients with granulomatous disease, such as sarcoidosis. Ingestion of escessive amounts of calcium, especially with alkali, may also cause hypercalcemia, as may prolonged immobilization, particularly under conditions of rapid bone turnover.", "contents": "Causes of hypercalcemia. Hypercalcemia is most commonly seen in normal infants as the result of normal rapid bone growth. The most common causative diseases are malignant disease and hyperparathyroidism. A variety of pharmacologic agents, especially vitamin D and its metabolites and thiazide diuretics, can elevate serum calcium levels. Hypersensitivity to vitamin D appears to be a cause of hypercalcemia in infants and in patients with granulomatous disease, such as sarcoidosis. Ingestion of escessive amounts of calcium, especially with alkali, may also cause hypercalcemia, as may prolonged immobilization, particularly under conditions of rapid bone turnover."}
{"id": "PMID:482180", "title": "Exercise and hormonal secretion.", "content": "The effect of graded exercise on the secretion of cortisol, testosterone, prolactin, growth hormone, thyroid stimulating hormone (TSH), luteinizing hormone and follicle stimulating hormone (FSH) is reported. While cortisol, prolactin, growth hormone and testosterone rise during the period of exercise, a rise in luteinizing hormone becomes evident only after rest. Levels of FSH and TSH remained unchanged.", "contents": "Exercise and hormonal secretion. The effect of graded exercise on the secretion of cortisol, testosterone, prolactin, growth hormone, thyroid stimulating hormone (TSH), luteinizing hormone and follicle stimulating hormone (FSH) is reported. While cortisol, prolactin, growth hormone and testosterone rise during the period of exercise, a rise in luteinizing hormone becomes evident only after rest. Levels of FSH and TSH remained unchanged."}
{"id": "PMID:482181", "title": "Diabetes insipidus, diabetes mellitus, optic atrophy and deafness. A clinical and genetic study.", "content": "Two Iraqi sisters and a female cousin developed diabetes insipidus (DI), diabetes mellitus (DM), optic atrophy (OA), and deafness (D), (the 'DIDMOAD' syndrome) before the age of 12 years. One girl exhibited all the features of this disease complex only 3 months after an unusually late onset of recognizable symptoms at 11 years 9 months. Another girl died suddenly and unexpectedly. This family study illustrates the recessive inheritance pattern of the syndrome.", "contents": "Diabetes insipidus, diabetes mellitus, optic atrophy and deafness. A clinical and genetic study. Two Iraqi sisters and a female cousin developed diabetes insipidus (DI), diabetes mellitus (DM), optic atrophy (OA), and deafness (D), (the 'DIDMOAD' syndrome) before the age of 12 years. One girl exhibited all the features of this disease complex only 3 months after an unusually late onset of recognizable symptoms at 11 years 9 months. Another girl died suddenly and unexpectedly. This family study illustrates the recessive inheritance pattern of the syndrome."}
{"id": "PMID:482183", "title": "Cimetidine in the prevention of gastric ulcer relapse.", "content": "Twenty-five patients with healed gastric ulcers completed an 11-month double-blind controlled trial comparing cimetidine 1 g daily with placebo in the prevention of ulcer relapse. Ulcers recurred in 14 patients, 12 on placebo, 2 on cimetidine. In 11 patients, 9 on cimetidine, 2 on placebo, the ulcers remained healed at the end of the trial (P less than 0.002). Cimetidine 1 g daily significantly reduces the relapse rate in gastric ulceration.", "contents": "Cimetidine in the prevention of gastric ulcer relapse. Twenty-five patients with healed gastric ulcers completed an 11-month double-blind controlled trial comparing cimetidine 1 g daily with placebo in the prevention of ulcer relapse. Ulcers recurred in 14 patients, 12 on placebo, 2 on cimetidine. In 11 patients, 9 on cimetidine, 2 on placebo, the ulcers remained healed at the end of the trial (P less than 0.002). Cimetidine 1 g daily significantly reduces the relapse rate in gastric ulceration."}
{"id": "PMID:482184", "title": "Cholelithiasis and biliary tract disease in sickle-cell disease in Nigerians.", "content": "The incidence of biliary tract disease was investigated by oral cholecystography and/or intravenous cholangiography in 77 unselected Nigerians with homozygous sickle-cell disease (SCD). They included 32 males and 45 females with ages ranging from 8 to 31 years. The prevalence of cholelithiasis was 9% with equal sex incidence. The stones were always multiple and 71.4% of them were radio opaque. Visualization and contractility of all gall bladders examined were normal. The only patient with symptoms and signs which suggested biliary tract disease had no radiographic evidence of cholelithiasis and it was impossible to predict the presence of cholelithiasis from the incidence of abdominal crises in the patients. The prevalence of cholelithiasis is significantly less than that reported in American patients with SCD (35%) and different dietary habits are probably responsible for this.", "contents": "Cholelithiasis and biliary tract disease in sickle-cell disease in Nigerians. The incidence of biliary tract disease was investigated by oral cholecystography and/or intravenous cholangiography in 77 unselected Nigerians with homozygous sickle-cell disease (SCD). They included 32 males and 45 females with ages ranging from 8 to 31 years. The prevalence of cholelithiasis was 9% with equal sex incidence. The stones were always multiple and 71.4% of them were radio opaque. Visualization and contractility of all gall bladders examined were normal. The only patient with symptoms and signs which suggested biliary tract disease had no radiographic evidence of cholelithiasis and it was impossible to predict the presence of cholelithiasis from the incidence of abdominal crises in the patients. The prevalence of cholelithiasis is significantly less than that reported in American patients with SCD (35%) and different dietary habits are probably responsible for this."}
{"id": "PMID:482185", "title": "The anal cushions--a fresh concept in diagnosis.", "content": "The anal canal is lined by cushions of specialized submucosal tissue which assist the continence mechanism. Starting with that premise, its various afflictions are examined and found to be more logically related, and much more readily understood.", "contents": "The anal cushions--a fresh concept in diagnosis. The anal canal is lined by cushions of specialized submucosal tissue which assist the continence mechanism. Starting with that premise, its various afflictions are examined and found to be more logically related, and much more readily understood."}
{"id": "PMID:482186", "title": "Feminizing adrenocortical carcinoma in a postmenopausal woman.", "content": "The successful surgical removal of a feminizing adrenal tumour in a postmenopausal woman is described. Most of these tumours are malignant and the pathological findings and the biological behaviour of the tumour frequently do not correlate, so that a careful follow-up is necessary. In the presence of vaginal bleeding in a postmenopausal woman endometrial carcinoma should be considered. If this has been ruled out and if oestrogens are elevated with low plasma gonadotrophins, a feminizing ovarian or adrenal tumour should be considered.", "contents": "Feminizing adrenocortical carcinoma in a postmenopausal woman. The successful surgical removal of a feminizing adrenal tumour in a postmenopausal woman is described. Most of these tumours are malignant and the pathological findings and the biological behaviour of the tumour frequently do not correlate, so that a careful follow-up is necessary. In the presence of vaginal bleeding in a postmenopausal woman endometrial carcinoma should be considered. If this has been ruled out and if oestrogens are elevated with low plasma gonadotrophins, a feminizing ovarian or adrenal tumour should be considered."}
{"id": "PMID:482187", "title": "Haematemesis during oral aminophylline treatment.", "content": "Two cases of gastrointestinal bleeding in patients being treated with continuous-release aminophylline tablets are reported.", "contents": "Haematemesis during oral aminophylline treatment. Two cases of gastrointestinal bleeding in patients being treated with continuous-release aminophylline tablets are reported."}
{"id": "PMID:482188", "title": "Neoplastic autovagotomy causing gastric stasis.", "content": "Neoplastic autovagotomy causing atonic gastric stasis is extremely rare. Two cases are reported in which marked gastric stasis was complicated by a bezoar and a gastric volvulus respectively. Both cases were associated with a left hilar bronchial carcinoma, and malignant invasion of the vagus nerve may have been the underlying cause.", "contents": "Neoplastic autovagotomy causing gastric stasis. Neoplastic autovagotomy causing atonic gastric stasis is extremely rare. Two cases are reported in which marked gastric stasis was complicated by a bezoar and a gastric volvulus respectively. Both cases were associated with a left hilar bronchial carcinoma, and malignant invasion of the vagus nerve may have been the underlying cause."}
{"id": "PMID:482189", "title": "Fulminating hyperlipidaemia.", "content": "A case is described of severe recurrent ischaemic heart disease associated with rapidly increasing and ultimately massive hyperlipidaemia. The case is discussed, with suggestions for management of similar problems.", "contents": "Fulminating hyperlipidaemia. A case is described of severe recurrent ischaemic heart disease associated with rapidly increasing and ultimately massive hyperlipidaemia. The case is discussed, with suggestions for management of similar problems."}
{"id": "PMID:482190", "title": "Paradoxical embolism associated with oral contraceptives: an underdiagnosed lesion?", "content": "A case of cerebral embolism is described in which paradoxical features have been proved clinically, and which may explain some hitherto unaccountable reported cases, and suggests the value of prospective studies. A successful outcome of such a proved case, associated with oral contraceptives, does not appear to have been reported previously.", "contents": "Paradoxical embolism associated with oral contraceptives: an underdiagnosed lesion? A case of cerebral embolism is described in which paradoxical features have been proved clinically, and which may explain some hitherto unaccountable reported cases, and suggests the value of prospective studies. A successful outcome of such a proved case, associated with oral contraceptives, does not appear to have been reported previously."}
{"id": "PMID:482191", "title": "Investigation of a case of prednisolone malabsorption.", "content": "Malabsorption of prednisolone administered as enteric coated tablets was suspected following therapeutic failure in an asthmatic. This was investigated by cortisol estimation and a Synacthen test and substantiated by the demonstration of abnormally low absorption of prednisolone from these tablets which nevertheless were normally absorbed by a volunteer. The absorption of prednisolone from conventional tablets in this patient was normal.", "contents": "Investigation of a case of prednisolone malabsorption. Malabsorption of prednisolone administered as enteric coated tablets was suspected following therapeutic failure in an asthmatic. This was investigated by cortisol estimation and a Synacthen test and substantiated by the demonstration of abnormally low absorption of prednisolone from these tablets which nevertheless were normally absorbed by a volunteer. The absorption of prednisolone from conventional tablets in this patient was normal."}
{"id": "PMID:482192", "title": "Carcinoid tumour as a complication of ulcerative colitis.", "content": "Patients with long-standing ulcerative colitis have an increased chance of developing a carcinoma of the colon, especially when the inflammatory process involves the entire colon, but no case of a carcinoid tumour of the colon occurring in a patient with ulcerative colitis has been reported.", "contents": "Carcinoid tumour as a complication of ulcerative colitis. Patients with long-standing ulcerative colitis have an increased chance of developing a carcinoma of the colon, especially when the inflammatory process involves the entire colon, but no case of a carcinoid tumour of the colon occurring in a patient with ulcerative colitis has been reported."}
{"id": "PMID:482193", "title": "Insulin immunology: humoral and cellular aspects in mono-component insulin treated patients.", "content": "A study on humoral and cellular immune response to monocomponent (MC) insulin is reported. Humoral insulin antibody did not reach detectable levels in 80% of patients studied. Two out of six patients in whom a detectable antibody response was found had bovine species specific antibody suggesting some contamination of the insulin preparation used. No evidence for lymphocyte transformation with MC insulin was observed in the patients treated. B component resulted in transformation in patients treated with non-MC insulins. Zinc acted as a stimulant to lymphocyte transformation in the control group. The significance of the observations is discussed.", "contents": "Insulin immunology: humoral and cellular aspects in mono-component insulin treated patients. A study on humoral and cellular immune response to monocomponent (MC) insulin is reported. Humoral insulin antibody did not reach detectable levels in 80% of patients studied. Two out of six patients in whom a detectable antibody response was found had bovine species specific antibody suggesting some contamination of the insulin preparation used. No evidence for lymphocyte transformation with MC insulin was observed in the patients treated. B component resulted in transformation in patients treated with non-MC insulins. Zinc acted as a stimulant to lymphocyte transformation in the control group. The significance of the observations is discussed."}
{"id": "PMID:482194", "title": "The dietary therapy of diabetes.", "content": "The basis of different diets for insulin dependent and maturity onset obese diabetics is discussed. The results achieved by strict calorie control in obese diabetics are compared with those in a group of patients with simple obesity and shown to be superior. The value of very low carbohydrate lager is assessed. It is concluded (a) that there is no case for severe carbohydrate restriction in diabetic diets unless there is a need for total calorie restriction and (b) 'Diet' low carbohydrate lager offers no particular advantages over ordinary lager in the diabetic diet.", "contents": "The dietary therapy of diabetes. The basis of different diets for insulin dependent and maturity onset obese diabetics is discussed. The results achieved by strict calorie control in obese diabetics are compared with those in a group of patients with simple obesity and shown to be superior. The value of very low carbohydrate lager is assessed. It is concluded (a) that there is no case for severe carbohydrate restriction in diabetic diets unless there is a need for total calorie restriction and (b) 'Diet' low carbohydrate lager offers no particular advantages over ordinary lager in the diabetic diet."}
{"id": "PMID:482195", "title": "Oral hypoglycaemic drugs--our current practice.", "content": "The pattern of prescribing in a diabetic clinic population extending over a 25 year period is examined. The number of patients studied, 4355, was divided into three categories according to age at onset of diabetes, those diagnosed before 15 years of age, those diagnosed between 15 and 45 years and those diagnosed after the age of 45 years. A reduction in the proportion of maturity-onset diabetics, in whom oral hypoglycaemic agents were used, is noted, when treatment prior to 1970 is compared with that post-1970. This reduction, from 36% of the total population prior to 1970 to 29% after 1970 was accompanied by an increase in the proportion treated by diet alone for the same period of from 17% to 30%. For the past three years metformin has replaced phenformin as the biguanide of choice in the clinic.", "contents": "Oral hypoglycaemic drugs--our current practice. The pattern of prescribing in a diabetic clinic population extending over a 25 year period is examined. The number of patients studied, 4355, was divided into three categories according to age at onset of diabetes, those diagnosed before 15 years of age, those diagnosed between 15 and 45 years and those diagnosed after the age of 45 years. A reduction in the proportion of maturity-onset diabetics, in whom oral hypoglycaemic agents were used, is noted, when treatment prior to 1970 is compared with that post-1970. This reduction, from 36% of the total population prior to 1970 to 29% after 1970 was accompanied by an increase in the proportion treated by diet alone for the same period of from 17% to 30%. For the past three years metformin has replaced phenformin as the biguanide of choice in the clinic."}
{"id": "PMID:482196", "title": "Diabetes and the insulin routine.", "content": "For a variety of reasons, animal insulin injected subcutaneously cannot replace completely the normal physiological mechanisms. Nevertheless, if the diabetic understands and follows his regime, good control is nearly always obtainable.", "contents": "Diabetes and the insulin routine. For a variety of reasons, animal insulin injected subcutaneously cannot replace completely the normal physiological mechanisms. Nevertheless, if the diabetic understands and follows his regime, good control is nearly always obtainable."}
{"id": "PMID:482197", "title": "Diabetic control--a critical evaluation of current methods of assessment.", "content": "The value of a number of currently used parameters in the assessment of diabetic control was critically assessed in one hundred and seventy seven diabetics. Each method was compared with the results of 5 blood glucose values taken at selected times during the 24 hours. In all diabetic patients, clinical assessment, semi-quantitative urine tests both at home and in hospital and 24-hour quantitative urinary glucose values can at best indicate only poor diabetic control. They failed to do this in a substantial number of cases. In insulin dependent diabetics, isolated blood glucose readings are of very limited value in predicting good diabetic control. In maturity onset type diabetes, a single blood glucose reading is a reliable index of diabetic control. The implications of these findings in the practical management of diabetes are considered.", "contents": "Diabetic control--a critical evaluation of current methods of assessment. The value of a number of currently used parameters in the assessment of diabetic control was critically assessed in one hundred and seventy seven diabetics. Each method was compared with the results of 5 blood glucose values taken at selected times during the 24 hours. In all diabetic patients, clinical assessment, semi-quantitative urine tests both at home and in hospital and 24-hour quantitative urinary glucose values can at best indicate only poor diabetic control. They failed to do this in a substantial number of cases. In insulin dependent diabetics, isolated blood glucose readings are of very limited value in predicting good diabetic control. In maturity onset type diabetes, a single blood glucose reading is a reliable index of diabetic control. The implications of these findings in the practical management of diabetes are considered."}
{"id": "PMID:482198", "title": "[Smoking habit at school. First habit at school. First results of a survey done in Marseille and its suburbs (author's transl)].", "content": "The authors report the first results of a transversal survey done from the end of November 1976 to the end of April 1977 in 4 secondary schools (CES, CEG) in Marseille and its suburbs. 1.795 children, between 11 and 15, in the first and fourth form, filled up a questionary inquiring into their smoking habits and consumption. Already a certain number of results can be drawn: --in this group of children, mostly girls (926 G/882 B) 181 were regular smokers (10,07%); --among these smokers, 70 smoked every day (38,6%), 60 smoked more than a packet a week (33%), finally 122 inhaled smoke; --they acquired the habit of smoking regularly between the age of 14 and 15, in the 3rd and 4th forms, as there was only 9,4% of smokers in the 3rd and 19% in the 4th form; --finally, parents seem to play a fundamental inducing part in this habit, as among smoking children 32.8% have parents both smoking, 41% a smoking mother, 71.2% a smoking father, while for non smokers the percentage were respectively of 19.9%, 24.9% and 54.3%.", "contents": "[Smoking habit at school. First habit at school. First results of a survey done in Marseille and its suburbs (author's transl)]. The authors report the first results of a transversal survey done from the end of November 1976 to the end of April 1977 in 4 secondary schools (CES, CEG) in Marseille and its suburbs. 1.795 children, between 11 and 15, in the first and fourth form, filled up a questionary inquiring into their smoking habits and consumption. Already a certain number of results can be drawn: --in this group of children, mostly girls (926 G/882 B) 181 were regular smokers (10,07%); --among these smokers, 70 smoked every day (38,6%), 60 smoked more than a packet a week (33%), finally 122 inhaled smoke; --they acquired the habit of smoking regularly between the age of 14 and 15, in the 3rd and 4th forms, as there was only 9,4% of smokers in the 3rd and 19% in the 4th form; --finally, parents seem to play a fundamental inducing part in this habit, as among smoking children 32.8% have parents both smoking, 41% a smoking mother, 71.2% a smoking father, while for non smokers the percentage were respectively of 19.9%, 24.9% and 54.3%."}
{"id": "PMID:482199", "title": "[Automatic continuous calculation in real time of alveolar ventilation (author's transl)].", "content": "The authors present the steps and rules to be respected for the building-up of a program of automatic calculation in real time of alveolar ventilation, with a programming calculator from the numerization of signals sent by a pneumotachograph and a rapid analyzer of carbon dioxide. Such a program would follow the rapid variations of alveolar ventilation in periods of transition or ventilatory instability. It could easily be adapted to the continuous calculation of carbon monoxide transfer by the method in a stable condition.", "contents": "[Automatic continuous calculation in real time of alveolar ventilation (author's transl)]. The authors present the steps and rules to be respected for the building-up of a program of automatic calculation in real time of alveolar ventilation, with a programming calculator from the numerization of signals sent by a pneumotachograph and a rapid analyzer of carbon dioxide. Such a program would follow the rapid variations of alveolar ventilation in periods of transition or ventilatory instability. It could easily be adapted to the continuous calculation of carbon monoxide transfer by the method in a stable condition."}
{"id": "PMID:482200", "title": "[Thoracoscopy in chronic pleural effusion (author's transl)].", "content": "123 patients with chronic pleurisy were investigated by thoracoscopy. The endoscopic biopsy is positive in 16 out of 17 cases of mesothelioma (96%), in 57 out of 72 cases of pleural metastasis (79%), and in 6 cases out of 7 pleural tuberculosis. Begnin pleural asbestosis was confirmed in 5. Endoscopic biopsy is thus more efficient than results obtained with pleural needle biopsy and exudative cytology. Thoracoscopy can be performed without any serious complications, this is not always true with open thoracotomy.", "contents": "[Thoracoscopy in chronic pleural effusion (author's transl)]. 123 patients with chronic pleurisy were investigated by thoracoscopy. The endoscopic biopsy is positive in 16 out of 17 cases of mesothelioma (96%), in 57 out of 72 cases of pleural metastasis (79%), and in 6 cases out of 7 pleural tuberculosis. Begnin pleural asbestosis was confirmed in 5. Endoscopic biopsy is thus more efficient than results obtained with pleural needle biopsy and exudative cytology. Thoracoscopy can be performed without any serious complications, this is not always true with open thoracotomy."}
{"id": "PMID:482201", "title": "[Cytology of chronic bronchitis: comparative study of sputum, brushing and bronchial biopsy (author's transl)].", "content": "Present research in chronic bronchitis is directed towards a technique of cytological detection. The object of the study was to compare the results of biopsy, brushing and sputum in 65 patients with clinical signs of chronic bronchitis. We established a good correlation between the results of biopsy and brushing. Unfortunately no reliable test for chronic bronchitis can be derived from the cytological study, which could indicate the future trend of the patient.", "contents": "[Cytology of chronic bronchitis: comparative study of sputum, brushing and bronchial biopsy (author's transl)]. Present research in chronic bronchitis is directed towards a technique of cytological detection. The object of the study was to compare the results of biopsy, brushing and sputum in 65 patients with clinical signs of chronic bronchitis. We established a good correlation between the results of biopsy and brushing. Unfortunately no reliable test for chronic bronchitis can be derived from the cytological study, which could indicate the future trend of the patient."}
{"id": "PMID:482202", "title": "[Corrective surgery of congenital chest malformations of the type Pectus excavatum and Pectus carinatum (author's transl)].", "content": "The authors presents an original process for surgical treatment of deformations of chest anterolateral wall, insisting on the procedures and techniques of the recommended process. The compulsory operative steps are: wide chondrectomy, retrosternal loosening, transversal osteotomy of the sternum and contention with retrosternal metallic plates. The author considers surgery to be necessary for all these anterolateral chest deformations and the best age for their correction to be after 10 to 12 years. The original technique for loosening and preserving the perichondrium in its wholeness is described together with the complete resection of deformed cartilages.", "contents": "[Corrective surgery of congenital chest malformations of the type Pectus excavatum and Pectus carinatum (author's transl)]. The authors presents an original process for surgical treatment of deformations of chest anterolateral wall, insisting on the procedures and techniques of the recommended process. The compulsory operative steps are: wide chondrectomy, retrosternal loosening, transversal osteotomy of the sternum and contention with retrosternal metallic plates. The author considers surgery to be necessary for all these anterolateral chest deformations and the best age for their correction to be after 10 to 12 years. The original technique for loosening and preserving the perichondrium in its wholeness is described together with the complete resection of deformed cartilages."}
{"id": "PMID:482203", "title": "[Mucoepidermoid tumors in the bronchi. About three cases (author's transl)].", "content": "The little frequent mucoepidermoid tumors in the bronchi can be distinguished by their histological characteristics associating epidermoid and mucilage-like cells identified through alcian blue and PAS colour agents. Although these tumors are normally considered as \"benign\", some of them may have a capacity to develop in very malign way. We enclose three new findings which all developped unfavorably in a short period.", "contents": "[Mucoepidermoid tumors in the bronchi. About three cases (author's transl)]. The little frequent mucoepidermoid tumors in the bronchi can be distinguished by their histological characteristics associating epidermoid and mucilage-like cells identified through alcian blue and PAS colour agents. Although these tumors are normally considered as \"benign\", some of them may have a capacity to develop in very malign way. We enclose three new findings which all developped unfavorably in a short period."}
{"id": "PMID:482204", "title": "[Study of two observations of bronchial muco-epidermoid tumour (author's transl)].", "content": "This study deals two cases of operated bronchial muco-epidermoid tumours. They were localized in both cases in the segmentary bronchi (apical ones of lower lobes), one mostly endo-bronchial, the other with an endo-exobronchial development. The histological diagnosis was done after the thorough analysis of the surgical specimen revealed the association of two cell types characteristic of these tumours: mucus producing cells and epidermoid cells. Evolution differs in both cases, as the first observation revealed the existence of a potentially benign tumour, while in the second it behaved like a truly malignant one with local recurrence, regional invasion and distant metastases.", "contents": "[Study of two observations of bronchial muco-epidermoid tumour (author's transl)]. This study deals two cases of operated bronchial muco-epidermoid tumours. They were localized in both cases in the segmentary bronchi (apical ones of lower lobes), one mostly endo-bronchial, the other with an endo-exobronchial development. The histological diagnosis was done after the thorough analysis of the surgical specimen revealed the association of two cell types characteristic of these tumours: mucus producing cells and epidermoid cells. Evolution differs in both cases, as the first observation revealed the existence of a potentially benign tumour, while in the second it behaved like a truly malignant one with local recurrence, regional invasion and distant metastases."}
{"id": "PMID:482205", "title": "[Radiological study of the chest involvement during angioimmunoblastic lymphadenitis (A.I.L.) Twenty observations (author's transl)].", "content": "From 20 observations, the authors worked out the frequency and the radiological picture of chest localizations of angio-immunoblastic lymphadenitis (A.I.L.). There are 2 kinds of pulmonary involvement: --a specific one by interstitial immunoblastic infiltration (20 to 45% of cases) and showing on X ray a reticulomicronodular interstitial syndrome; --a secondary infection (33% of cases) often the cause of death. Pleural involvement is unfrequent (11% to 25% of cases) and its onset late. Mediastinal lymph-node involvement is found in 37 to 50% of cases. It is usually discovered straight away.", "contents": "[Radiological study of the chest involvement during angioimmunoblastic lymphadenitis (A.I.L.) Twenty observations (author's transl)]. From 20 observations, the authors worked out the frequency and the radiological picture of chest localizations of angio-immunoblastic lymphadenitis (A.I.L.). There are 2 kinds of pulmonary involvement: --a specific one by interstitial immunoblastic infiltration (20 to 45% of cases) and showing on X ray a reticulomicronodular interstitial syndrome; --a secondary infection (33% of cases) often the cause of death. Pleural involvement is unfrequent (11% to 25% of cases) and its onset late. Mediastinal lymph-node involvement is found in 37 to 50% of cases. It is usually discovered straight away."}
{"id": "PMID:482206", "title": "[Segmentary bronchial atresia. One observation (author's transl)].", "content": "This study was done on a case of segmentary bronchial atresia of the left upper lobe. It is an exceptional congenital malformation, as only 47 observations have been so far recorded. Diagnosis was essentially radiological. The standard chest X ray and tomographies revealed an area of segmentary and lobar transparency surrounding a para-hilar lump. Diagnosis was confirmed by bronchography revealing the lack of injection into the atretic bronchus. Possible infectious complications and the evolution of bronchiectasis and obstructive emphysema justify the surgical exeresis.", "contents": "[Segmentary bronchial atresia. One observation (author's transl)]. This study was done on a case of segmentary bronchial atresia of the left upper lobe. It is an exceptional congenital malformation, as only 47 observations have been so far recorded. Diagnosis was essentially radiological. The standard chest X ray and tomographies revealed an area of segmentary and lobar transparency surrounding a para-hilar lump. Diagnosis was confirmed by bronchography revealing the lack of injection into the atretic bronchus. Possible infectious complications and the evolution of bronchiectasis and obstructive emphysema justify the surgical exeresis."}
{"id": "PMID:482243", "title": "A novel antenatal record to help midwives.", "content": "An antenatal questionnaire and graph for plotting weight and fundal height gives immediate warning of women at risk in pregnancy in a simple and visually striking way. It saves midwives' time, improves their clinical awareness and gives them more confidence in referring patients.", "contents": "A novel antenatal record to help midwives. An antenatal questionnaire and graph for plotting weight and fundal height gives immediate warning of women at risk in pregnancy in a simple and visually striking way. It saves midwives' time, improves their clinical awareness and gives them more confidence in referring patients."}
{"id": "PMID:482306", "title": "Delta 9-tetrahydrocannabinol: elevation of absolute visual thresholds of rabbits.", "content": "The effect of Delta 9-tetrahydrocannabinol (THC) on the ability of rabbits to detect a minimal light stimulus (absolute visual threshold) was examined using the method of limits with an aversive classical conditioning paradigm. Both of two dosage levels of THC, similar to an amount ingested by a human from a single cigarette, significantly elevated the absolute visual threshold of all animals. Normal baseline thresholds, however, returned with 24 hours.", "contents": "Delta 9-tetrahydrocannabinol: elevation of absolute visual thresholds of rabbits. The effect of Delta 9-tetrahydrocannabinol (THC) on the ability of rabbits to detect a minimal light stimulus (absolute visual threshold) was examined using the method of limits with an aversive classical conditioning paradigm. Both of two dosage levels of THC, similar to an amount ingested by a human from a single cigarette, significantly elevated the absolute visual threshold of all animals. Normal baseline thresholds, however, returned with 24 hours."}
{"id": "PMID:482307", "title": "Metabolic and pharmacodynamic tolerance to ethanol in rats.", "content": "The development of tolerance to ethanol was studied in rats fed nutritionally adequate liquid diets containing ethanol or sucrose for up to 5 weeks. Tolerance was shown to be due to both metabolic and pharmacodynamic factors. Tolerance began to develop after 3 days of ethanol intake, reached a plateau by 16 days and persisted for up to 22 days after stopping the ethanol intake. The rate of onset and decay of both components of tolerance was similar.", "contents": "Metabolic and pharmacodynamic tolerance to ethanol in rats. The development of tolerance to ethanol was studied in rats fed nutritionally adequate liquid diets containing ethanol or sucrose for up to 5 weeks. Tolerance was shown to be due to both metabolic and pharmacodynamic factors. Tolerance began to develop after 3 days of ethanol intake, reached a plateau by 16 days and persisted for up to 22 days after stopping the ethanol intake. The rate of onset and decay of both components of tolerance was similar."}
{"id": "PMID:482308", "title": "Adrenal demedullation and peripheral 6-OHDA administration in the rabbit: effects on body weight, general activity and cardiovascular responsivity.", "content": "Two experiments were undertaken to determine the effects of systemic 6-hydroxydopamine (6-OHDA) administration and adrenal enucleation on body weight, brain and heart norepinephrine, general activity, and cardiovascular responsivity in the rabbit. 6-Hydroxydopamine produced long lasting, dose related decreases in baseline blood pressure (BP) and in pressor responses following electric shock. Partial reversal of the BP changes was observed in nonenucleated animals tested 20 days after 6-OHDA administration but not in enucleated animals tested 5, 10 or 20 days post-injection or in control animals tested 5, 10 or 15 days after vehicle injection. Although 6-OHDA administration produced transient weight loss and activity decrements, no long term weight or activity changes were observed.", "contents": "Adrenal demedullation and peripheral 6-OHDA administration in the rabbit: effects on body weight, general activity and cardiovascular responsivity. Two experiments were undertaken to determine the effects of systemic 6-hydroxydopamine (6-OHDA) administration and adrenal enucleation on body weight, brain and heart norepinephrine, general activity, and cardiovascular responsivity in the rabbit. 6-Hydroxydopamine produced long lasting, dose related decreases in baseline blood pressure (BP) and in pressor responses following electric shock. Partial reversal of the BP changes was observed in nonenucleated animals tested 20 days after 6-OHDA administration but not in enucleated animals tested 5, 10 or 20 days post-injection or in control animals tested 5, 10 or 15 days after vehicle injection. Although 6-OHDA administration produced transient weight loss and activity decrements, no long term weight or activity changes were observed."}
{"id": "PMID:482309", "title": "Learning ability in adult female rats perinatally exposed to methadone.", "content": "Cognitive functioning of adult female rats that were maternally exposed to methadone (5 mg/kg daily) during gestation and/or lactation was studied by assessing performance on a food-motivated light-dark discrimination learning test and on active and passive shock-avoidance tests. Methadone-exposed rats exhibited difficulties on the light-dark discrimination learning and the active avoidance tests, and behavioral deficits appeared to be related to the timing and duration of drug treatment. On the light-dark discrimination test only 33% of the rats in the gestation group and 25% of the animals in the lactation group met criterion in comparison to 87% of the control rats. Thirty-three percent of the animals in either the gestation or gestation-lactation groups met criterion on the active avoidance test in contrast to 87% of the controls. These data suggest that perinatal exposure to methadone impairs cognitive abilities in the adult female rat.", "contents": "Learning ability in adult female rats perinatally exposed to methadone. Cognitive functioning of adult female rats that were maternally exposed to methadone (5 mg/kg daily) during gestation and/or lactation was studied by assessing performance on a food-motivated light-dark discrimination learning test and on active and passive shock-avoidance tests. Methadone-exposed rats exhibited difficulties on the light-dark discrimination learning and the active avoidance tests, and behavioral deficits appeared to be related to the timing and duration of drug treatment. On the light-dark discrimination test only 33% of the rats in the gestation group and 25% of the animals in the lactation group met criterion in comparison to 87% of the control rats. Thirty-three percent of the animals in either the gestation or gestation-lactation groups met criterion on the active avoidance test in contrast to 87% of the controls. These data suggest that perinatal exposure to methadone impairs cognitive abilities in the adult female rat."}
{"id": "PMID:482310", "title": "Prostaglandin E1 inhibits acute cell dehydration thirst.", "content": "Intraperitoneally injected PGE1 (100 micrograms/Kg) inhibits specifically the drinking induced by both IP and IV 2 M NaCl (6 ml/Kg) and compound 48/80 (100 micrograms/Kg, IP). Probenecid (150 mg/Kg, IP) which is not a dipsogen, has no effect on the PGE1 induced inhibition of acute cell dehydration thirst. It is concluded the PGE1 acts upon the peripheral mast cells, inhibiting their secretion and thus affecting the water intake associated with the activation of these cells either by hypertonicity or specific stimulants of amine release. These results raise the possibility that endogenous prostaglandins might be involved in the modulation of some of the signals which convey to the brain information on the tonicity of the body fluids.", "contents": "Prostaglandin E1 inhibits acute cell dehydration thirst. Intraperitoneally injected PGE1 (100 micrograms/Kg) inhibits specifically the drinking induced by both IP and IV 2 M NaCl (6 ml/Kg) and compound 48/80 (100 micrograms/Kg, IP). Probenecid (150 mg/Kg, IP) which is not a dipsogen, has no effect on the PGE1 induced inhibition of acute cell dehydration thirst. It is concluded the PGE1 acts upon the peripheral mast cells, inhibiting their secretion and thus affecting the water intake associated with the activation of these cells either by hypertonicity or specific stimulants of amine release. These results raise the possibility that endogenous prostaglandins might be involved in the modulation of some of the signals which convey to the brain information on the tonicity of the body fluids."}
{"id": "PMID:482312", "title": "Effects of psychotropic drugs on pressor and behavioral responses to brain stimulation in unrestrained, unanesthetized rats.", "content": "Electrical stimulation of the posterior hypothalamus (PH) and the mesencephalic reticular formation (MRF) in unanesthetized, unrestrained rats with chronic electrode implants and an arterial cannula elicited a rise in blood pressure accompanied by behavioral changes such as exploration, flight or escape responses. Pentobarbital inhibited both the pressor and behavioral responses to PH and MRF stimulation. Chlorpormazine, diazepam and imipramine depressed the pressor response to PH stimulation rather than that to MRF stimulation with affecting the behavioral responses. It is concluded from these results that chlorpromazine, diazepam and imipramine exert their action on the neural pathway involved in the pressor response rather than on that inducing behavioral responses, whereas pentobarbital affects more extended brain areas related to these neural systems.", "contents": "Effects of psychotropic drugs on pressor and behavioral responses to brain stimulation in unrestrained, unanesthetized rats. Electrical stimulation of the posterior hypothalamus (PH) and the mesencephalic reticular formation (MRF) in unanesthetized, unrestrained rats with chronic electrode implants and an arterial cannula elicited a rise in blood pressure accompanied by behavioral changes such as exploration, flight or escape responses. Pentobarbital inhibited both the pressor and behavioral responses to PH and MRF stimulation. Chlorpormazine, diazepam and imipramine depressed the pressor response to PH stimulation rather than that to MRF stimulation with affecting the behavioral responses. It is concluded from these results that chlorpromazine, diazepam and imipramine exert their action on the neural pathway involved in the pressor response rather than on that inducing behavioral responses, whereas pentobarbital affects more extended brain areas related to these neural systems."}
{"id": "PMID:482313", "title": "Inhibition of the action of anticonvulsants by lithium treatment.", "content": "The present study demonstrates that the effectiveness of anticonvulsants significantly decreases following lithium treatment (3 doses of 50 mg/kg of LiCl given every 12 hr). The anticonvulsant action of phenytoin, phenobarbital and of 3 carbonic anhydrase inhibitors: methazolamide, acetazolamide, ethoxzolamide, was assessed in rats subjected to maximal electroshock. In a chronic study on tolerance development to the anticonvulsant action of acetazolamide, lithium treatment (10 mg/kg per day) has been shown to inhibit gradually the action of acetazolamide. These results are consistent with the hypothesis that norepinephrine and dopamine are involved in the action of anticonvulsants.", "contents": "Inhibition of the action of anticonvulsants by lithium treatment. The present study demonstrates that the effectiveness of anticonvulsants significantly decreases following lithium treatment (3 doses of 50 mg/kg of LiCl given every 12 hr). The anticonvulsant action of phenytoin, phenobarbital and of 3 carbonic anhydrase inhibitors: methazolamide, acetazolamide, ethoxzolamide, was assessed in rats subjected to maximal electroshock. In a chronic study on tolerance development to the anticonvulsant action of acetazolamide, lithium treatment (10 mg/kg per day) has been shown to inhibit gradually the action of acetazolamide. These results are consistent with the hypothesis that norepinephrine and dopamine are involved in the action of anticonvulsants."}
{"id": "PMID:482315", "title": "Biochemical aspects of protein synthesis inhibition by cycloheximide in one or both hemispheres of the chick brain.", "content": "Instracrnial administration of cycloheximide into one hemisphere of the chick brain resulted in inhibition of 14C-leucine incorporation into protein only in that hemisphere when the labelled amino acid was administered intracranially. With pericardial injections of labelled amino acid, inhibition of 14C-leucine incorporation was obtained in both the CXM-treated and the untreated hemisphere, when compared with bilateral saline-treated controls. The levels of inhibition were comparable to those obtained with bilateral administration of CXM. There was, however, a slight but significantly higher level of inhibition in the CXM-treated hemisphere. The results were interpreted as supporting the conclusion that monocular learning in chicks resulted in the formation of an engram only in the trained hemisphere.", "contents": "Biochemical aspects of protein synthesis inhibition by cycloheximide in one or both hemispheres of the chick brain. Instracrnial administration of cycloheximide into one hemisphere of the chick brain resulted in inhibition of 14C-leucine incorporation into protein only in that hemisphere when the labelled amino acid was administered intracranially. With pericardial injections of labelled amino acid, inhibition of 14C-leucine incorporation was obtained in both the CXM-treated and the untreated hemisphere, when compared with bilateral saline-treated controls. The levels of inhibition were comparable to those obtained with bilateral administration of CXM. There was, however, a slight but significantly higher level of inhibition in the CXM-treated hemisphere. The results were interpreted as supporting the conclusion that monocular learning in chicks resulted in the formation of an engram only in the trained hemisphere."}
{"id": "PMID:482316", "title": "Dissimilar effects of lithium isotopes on motility in rats.", "content": "Male Wistar rats were injected twice daily with chlorides of pure lithium isotopes, either Li-6 or Li-7. While both salts decreased mobility, the salt of Li-6 initially produced a more profound effect than the salt of Li-7. This differential effect of the isotopes proved to be time dependent and was most evident on the third day of treatment. Li-6 is likely to become a valuable tool in basic psychopharmacological research. It may possess therapeutic and/or toxic properties that are different from those of lithium salts currently used in treatment of mania.", "contents": "Dissimilar effects of lithium isotopes on motility in rats. Male Wistar rats were injected twice daily with chlorides of pure lithium isotopes, either Li-6 or Li-7. While both salts decreased mobility, the salt of Li-6 initially produced a more profound effect than the salt of Li-7. This differential effect of the isotopes proved to be time dependent and was most evident on the third day of treatment. Li-6 is likely to become a valuable tool in basic psychopharmacological research. It may possess therapeutic and/or toxic properties that are different from those of lithium salts currently used in treatment of mania."}
{"id": "PMID:482317", "title": "Meperidine does not block the cholinergic effects of oxotremorine.", "content": "Meperidine (20 and 40 mg/kg, IP) did not block the cholinergic effects (tremor, salivation, and tearing) produced by oxotremorine (0.4 mg/kg, SC) in rats, whereas atropine blocked these three effects (10 mg/kg, 2.5 mg/kg and 0.08 mg/kg, respectively.", "contents": "Meperidine does not block the cholinergic effects of oxotremorine. Meperidine (20 and 40 mg/kg, IP) did not block the cholinergic effects (tremor, salivation, and tearing) produced by oxotremorine (0.4 mg/kg, SC) in rats, whereas atropine blocked these three effects (10 mg/kg, 2.5 mg/kg and 0.08 mg/kg, respectively."}
{"id": "PMID:482318", "title": "Cyclobenzaprine and ethanol interaction.", "content": "The effects of cyclobenzaprine, a tricyclic compound, on the central depressant action of ethanol and on hepatic ethanol metabolizing enzymes were studied in rodents. Administration of cyclobenzaprine, 5 mg/kg, IP, 30 min prior to a narcotic dose of ethanol solution, 5 g/kg, IP, enhanced ethanol-produced narcosis in mice. This effect was greater in male than in female mice. Cyclobenzaprine inhibited endogenous rat liver alcohol dehydrogenase in vitro in the concentration range between 10(-5) M and 10(-6)M. Cyclobenzaprine exerted little effect on hepatic aldehyde dehydrogenase in vitro. The results suggest that cyclobenzaprine possesses depressant properties and inhibition of liver alcohol dehydrogenase may underlie the observed behavioral response studied. It is concluded that alteration of endogenous liver alcohol dehydrogenase by certain tricyclic antidepressant drugs may be involved in the mechanism(s) of their toxic interaction with ethanol.", "contents": "Cyclobenzaprine and ethanol interaction. The effects of cyclobenzaprine, a tricyclic compound, on the central depressant action of ethanol and on hepatic ethanol metabolizing enzymes were studied in rodents. Administration of cyclobenzaprine, 5 mg/kg, IP, 30 min prior to a narcotic dose of ethanol solution, 5 g/kg, IP, enhanced ethanol-produced narcosis in mice. This effect was greater in male than in female mice. Cyclobenzaprine inhibited endogenous rat liver alcohol dehydrogenase in vitro in the concentration range between 10(-5) M and 10(-6)M. Cyclobenzaprine exerted little effect on hepatic aldehyde dehydrogenase in vitro. The results suggest that cyclobenzaprine possesses depressant properties and inhibition of liver alcohol dehydrogenase may underlie the observed behavioral response studied. It is concluded that alteration of endogenous liver alcohol dehydrogenase by certain tricyclic antidepressant drugs may be involved in the mechanism(s) of their toxic interaction with ethanol."}
{"id": "PMID:482327", "title": "[Carbonyl derivatives of tilorone. Part 2: On the cancerostatic activity of carbonyl derivatives of tilorone (author's transl)].", "content": "A series of carbonyl derivatives of tilorone was synthetized by reaction with appropriate amino compounds, mainly hydrazines and hydrazides. The condensation products obtained were tested for cancerostatic activity against the murine leucaemia L 1210 and the Walker carcinosarcoma of the rat. Only three of the substances under investigation (1a, 5, 13) proved active against the Walker carcinosarcoma, one of which (5) being comparable to tilorone. No activity against L 1210 was observed, even tilorone exerted no effect. The reduction in activity against the Walker carcinosarcoma which resulted from the carbonyl substitution might be caused by a decrease in the ability to intercalate into DNA.", "contents": "[Carbonyl derivatives of tilorone. Part 2: On the cancerostatic activity of carbonyl derivatives of tilorone (author's transl)]. A series of carbonyl derivatives of tilorone was synthetized by reaction with appropriate amino compounds, mainly hydrazines and hydrazides. The condensation products obtained were tested for cancerostatic activity against the murine leucaemia L 1210 and the Walker carcinosarcoma of the rat. Only three of the substances under investigation (1a, 5, 13) proved active against the Walker carcinosarcoma, one of which (5) being comparable to tilorone. No activity against L 1210 was observed, even tilorone exerted no effect. The reduction in activity against the Walker carcinosarcoma which resulted from the carbonyl substitution might be caused by a decrease in the ability to intercalate into DNA."}
{"id": "PMID:482328", "title": "[Biologically active azomethines. Del\u00e9pine reaction with 1-chloromethylisatin; preparation of some 1-aminomethylisatinazomethines (author's transl)].", "content": "The authors describe the synthesis of I-aminomethylisatin (3) from [I-chloromethylisatin] (1) by means of the Del\u00e9pine reaction. On condensation with aromatic aldehydes, 3 yields the azomethines 5--7. The azomethine 5 is converted into the corresponding thiosemicarbazone 9. Testing for mitodepressive activity showed that the compounds 5--7 are active. On testing in vitro for tuberculostatic effects, the compound 9 proved exceptionally potent.", "contents": "[Biologically active azomethines. Del\u00e9pine reaction with 1-chloromethylisatin; preparation of some 1-aminomethylisatinazomethines (author's transl)]. The authors describe the synthesis of I-aminomethylisatin (3) from [I-chloromethylisatin] (1) by means of the Del\u00e9pine reaction. On condensation with aromatic aldehydes, 3 yields the azomethines 5--7. The azomethine 5 is converted into the corresponding thiosemicarbazone 9. Testing for mitodepressive activity showed that the compounds 5--7 are active. On testing in vitro for tuberculostatic effects, the compound 9 proved exceptionally potent."}
{"id": "PMID:482329", "title": "Evaluation of certain pharmaceuticals with dibromodimethylhydantoin. Part 2: Determination of analgesics.", "content": "1,3-Dibromodimethylhydantoin (DBH) is a stable dihalogen reagent. It can be used as an analytical reagent for certain analgesics. It gives stoichiometric results by direct titration in acid medium. The end point is detected visually or potentiometrically. Also spectrophotometric titrations have been attempted at 345 nm. In all cases the results of the analysis of the drugs studied either in the pure state or in their dosage forms comply with those given by N-bromo succinimide (NBS) and by the official methods, however this application is distinguished by simplicity and accuracy.", "contents": "Evaluation of certain pharmaceuticals with dibromodimethylhydantoin. Part 2: Determination of analgesics. 1,3-Dibromodimethylhydantoin (DBH) is a stable dihalogen reagent. It can be used as an analytical reagent for certain analgesics. It gives stoichiometric results by direct titration in acid medium. The end point is detected visually or potentiometrically. Also spectrophotometric titrations have been attempted at 345 nm. In all cases the results of the analysis of the drugs studied either in the pure state or in their dosage forms comply with those given by N-bromo succinimide (NBS) and by the official methods, however this application is distinguished by simplicity and accuracy."}
{"id": "PMID:482330", "title": "[Comparative studies on the stability of aqueous drug solutions in the isothermal and the non-isothermal short-time test as well as in the long-time test. Part 2: The stability of aqueous tetracaine solutions in the non-isothermal short-time test (author's transl)].", "content": "A study of the hydrolytic degradation of tetracaine solutions at various pH values demonstrates that the results from non-isothermal stability testing with logarithmic rise in temperature are in good agreement with the activation energies determined, under analogous conditions, by means of the isothermal short-time test and long-time test. The range of the maximum of stability is more clearly evinced by the non-isothermal short-time test than by the isothermal stability test. The comparison of the two methods reveals that the deviation of the reaction rate constants is greater in the non-isothermal test, which is due to the calculation required for the logarithmic rise in temperature. The results obtained with tetracaine evidence that the non-isothermal stability test is an appropriate method for the rapid determination of stability parameters (e.g. stability maximum, hydrolysis velocities) in the frame-work of testing potential drugs for stability and in the optimization of prescriptions.", "contents": "[Comparative studies on the stability of aqueous drug solutions in the isothermal and the non-isothermal short-time test as well as in the long-time test. Part 2: The stability of aqueous tetracaine solutions in the non-isothermal short-time test (author's transl)]. A study of the hydrolytic degradation of tetracaine solutions at various pH values demonstrates that the results from non-isothermal stability testing with logarithmic rise in temperature are in good agreement with the activation energies determined, under analogous conditions, by means of the isothermal short-time test and long-time test. The range of the maximum of stability is more clearly evinced by the non-isothermal short-time test than by the isothermal stability test. The comparison of the two methods reveals that the deviation of the reaction rate constants is greater in the non-isothermal test, which is due to the calculation required for the logarithmic rise in temperature. The results obtained with tetracaine evidence that the non-isothermal stability test is an appropriate method for the rapid determination of stability parameters (e.g. stability maximum, hydrolysis velocities) in the frame-work of testing potential drugs for stability and in the optimization of prescriptions."}
{"id": "PMID:482331", "title": "[On the polymorphism of barbiturates in powders and tablets. Part 2: On the infrared spectroscopy and the X-ray diffractometry of barbital (author's transl)].", "content": "By means of IR spectroscopy and X-ray diffractometry, two commercial brands of barbital and four barbital substances recrystallized from water or an acetone-water mixture were examined for polymorphism. The results obtained demonstrate that these substances differ in crystal lattice and that there are six polymorphic modifications.", "contents": "[On the polymorphism of barbiturates in powders and tablets. Part 2: On the infrared spectroscopy and the X-ray diffractometry of barbital (author's transl)]. By means of IR spectroscopy and X-ray diffractometry, two commercial brands of barbital and four barbital substances recrystallized from water or an acetone-water mixture were examined for polymorphism. The results obtained demonstrate that these substances differ in crystal lattice and that there are six polymorphic modifications."}
{"id": "PMID:482332", "title": "Bioavailability of phenytoin in dogs: effect of crystal form and particle size.", "content": "Significant differences in bioavailability were observed in dogs when phenytoin crystals of different particle size were administered. No significant differences in bioavailability were observed when different crystal forms were compared.", "contents": "Bioavailability of phenytoin in dogs: effect of crystal form and particle size. Significant differences in bioavailability were observed in dogs when phenytoin crystals of different particle size were administered. No significant differences in bioavailability were observed when different crystal forms were compared."}
{"id": "PMID:482333", "title": "[Studies on the kinetics of methaqualone during the late stage of pregnancy (author's transl)].", "content": "After oral administration of 0.4 g of methaqualone to pregnant and non-pregnant women, the authors stated that the half-life of this drug was significantly reduced during the last trimester of pregnancy. The two experimental groups showed no significant differences in the volume of distribution and the fictitious initial concentration. The commonly used therapeutical dosages are not likely to produce increased blood levels during normal pregnancy.", "contents": "[Studies on the kinetics of methaqualone during the late stage of pregnancy (author's transl)]. After oral administration of 0.4 g of methaqualone to pregnant and non-pregnant women, the authors stated that the half-life of this drug was significantly reduced during the last trimester of pregnancy. The two experimental groups showed no significant differences in the volume of distribution and the fictitious initial concentration. The commonly used therapeutical dosages are not likely to produce increased blood levels during normal pregnancy."}
{"id": "PMID:482340", "title": "Osmolality/osmolarity data and calculations.", "content": "In view of the increasing interest in the osmolarity of parenteral solutions, an extensive compilation of pertinent numerical data is described. This compilation tabulates values of density, molar concentration, freezing point lowering,real osmolality, and molar concentration of the isotonic sodium chloride solution for 99 compounds, two-thirds of which are of use in pharmacy. A wide range of concentrations is covered for each compound, the values being listed in increments of 0.50, 1.00, or 2.00 percent w/w. Simple equations to convert molar into molal concentrations and to correlate freezing point lowering, osmotic pressure, osmolality and molality are presented. The practical use and theoretical meaning of the osmotic coefficient are discussed. As an example of a solution with several solutes, the real osmolality and osmolarity of Ringer's Solution is computed from the tabulated values of the freezing point depressions of the component salts.", "contents": "Osmolality/osmolarity data and calculations. In view of the increasing interest in the osmolarity of parenteral solutions, an extensive compilation of pertinent numerical data is described. This compilation tabulates values of density, molar concentration, freezing point lowering,real osmolality, and molar concentration of the isotonic sodium chloride solution for 99 compounds, two-thirds of which are of use in pharmacy. A wide range of concentrations is covered for each compound, the values being listed in increments of 0.50, 1.00, or 2.00 percent w/w. Simple equations to convert molar into molal concentrations and to correlate freezing point lowering, osmotic pressure, osmolality and molality are presented. The practical use and theoretical meaning of the osmotic coefficient are discussed. As an example of a solution with several solutes, the real osmolality and osmolarity of Ringer's Solution is computed from the tabulated values of the freezing point depressions of the component salts."}
{"id": "PMID:482342", "title": "Plasma protein displacement interactions are rarely of clinical significance.", "content": "Drug interactions in general have been overemphasized with respect to both frequency and clinical importance. Interactions allegedly based on displacement of highly protein-bound drugs from plasma proteins are particularly misunderstood and the evidence supporting such interaction is typically incomplete. In those interactions where displacement has been most convincingly shown and a clinically important consequence ensues, an additional interaction mechanism has been present. On theoretical grounds such simple displacement interactions are not likely to be common, easy to detect or serious. If such interactions occur they will be transient.", "contents": "Plasma protein displacement interactions are rarely of clinical significance. Drug interactions in general have been overemphasized with respect to both frequency and clinical importance. Interactions allegedly based on displacement of highly protein-bound drugs from plasma proteins are particularly misunderstood and the evidence supporting such interaction is typically incomplete. In those interactions where displacement has been most convincingly shown and a clinically important consequence ensues, an additional interaction mechanism has been present. On theoretical grounds such simple displacement interactions are not likely to be common, easy to detect or serious. If such interactions occur they will be transient."}
{"id": "PMID:482343", "title": "Binding of adrenaline and isoprenaline to plasma proteins of the dog.", "content": "Adrenaline was incubated with dog plasma in concentrations ranging from 50 to 1,000 ng/ml and binding to plasma proteins was found to be about 12% (8, 12 or 14%, as determined by spectrophotofluorimetric, liquid scintillation or Sephadex G-50 gel filtration methods, respectively). For isoprenaline, the binding to plasma proteins was of about 14-18%, as shown by liquid scintillation or Sephadex G-50 gel filtration methods. Cellulose acetate electrophoresis demonstrated that albumin does not play any significant role in this binding, in the case of both adrenaline and isoprenaline. These results show that adrenaline and isoprenaline are much less bound to proteins that noradrenaline (40%). It is concluded that the N substitution present in adrenaline and isoprenaline appears to be responsible for the different degrees of protein binding.", "contents": "Binding of adrenaline and isoprenaline to plasma proteins of the dog. Adrenaline was incubated with dog plasma in concentrations ranging from 50 to 1,000 ng/ml and binding to plasma proteins was found to be about 12% (8, 12 or 14%, as determined by spectrophotofluorimetric, liquid scintillation or Sephadex G-50 gel filtration methods, respectively). For isoprenaline, the binding to plasma proteins was of about 14-18%, as shown by liquid scintillation or Sephadex G-50 gel filtration methods. Cellulose acetate electrophoresis demonstrated that albumin does not play any significant role in this binding, in the case of both adrenaline and isoprenaline. These results show that adrenaline and isoprenaline are much less bound to proteins that noradrenaline (40%). It is concluded that the N substitution present in adrenaline and isoprenaline appears to be responsible for the different degrees of protein binding."}
{"id": "PMID:482344", "title": "Modification of D-galactosamine-induced liver injury in the rat by spironolactone.", "content": "Spironolactone, a competitive inhibitor of mineralocorticoid effects on the distal tubule of the kidney, has recently been found to have other metabolic effects. In these studies, spironolactone (200mg/kg intraperitoneally) for 3 days was found to have a marked protective effect against the hepatotoxic effect of D-galactosamine (275 mg/kg) in rats. Further progress in defining the mechanism of protection from D-galactosamine hepatic necrosis by spironolactone will require assessment of effects of spironolactone on uridine nucleotide metabolism.", "contents": "Modification of D-galactosamine-induced liver injury in the rat by spironolactone. Spironolactone, a competitive inhibitor of mineralocorticoid effects on the distal tubule of the kidney, has recently been found to have other metabolic effects. In these studies, spironolactone (200mg/kg intraperitoneally) for 3 days was found to have a marked protective effect against the hepatotoxic effect of D-galactosamine (275 mg/kg) in rats. Further progress in defining the mechanism of protection from D-galactosamine hepatic necrosis by spironolactone will require assessment of effects of spironolactone on uridine nucleotide metabolism."}
{"id": "PMID:482345", "title": "Comparative effects of delta 9-tetrahydrocannabinol, tetrabenazine and chlorpromazine on rectal temperature in the rat.", "content": "Tetrabenazine evoked a biphasic response in rectal temperature of the rat following intraperitoneal administration of 4.4--70.0 mg/kg. The initial hyperthermia was inversely related to the dose while the subsequent hypothermia was dose-dependent. This biphasic effect on temperature was similar to that seen with THC at 0.5 mg/kg, thus adding further evidence of a similarity in the mode of action of the two drugs. In contrast, only hypothermia was evident following chlorpromazine administration.", "contents": "Comparative effects of delta 9-tetrahydrocannabinol, tetrabenazine and chlorpromazine on rectal temperature in the rat. Tetrabenazine evoked a biphasic response in rectal temperature of the rat following intraperitoneal administration of 4.4--70.0 mg/kg. The initial hyperthermia was inversely related to the dose while the subsequent hypothermia was dose-dependent. This biphasic effect on temperature was similar to that seen with THC at 0.5 mg/kg, thus adding further evidence of a similarity in the mode of action of the two drugs. In contrast, only hypothermia was evident following chlorpromazine administration."}
{"id": "PMID:482346", "title": "Muscarinic receptors of the pancreas: a correlation between displacement of (3 H)-quinuclidinyl benzilate binding and amylase secretion.", "content": "(3H)-quinuclidinyl benzilate [(3H)-QNB], A potent muscarinic antagonist, was used as a tool to reveal the presence of muscarinic receptors in rat pancreas. Binding assays were performed on a 49,000 g pellet. Known muscarinic antagonists and agonists were used for competition assays on (3N)-QNB binding and studies on amylase secretion in vitro. A correlation was established between the binding assays and the studies on amylase release which suggests that the binding sites and those involved in the cholinergic control of pancreatic enzyme secretion are similar.", "contents": "Muscarinic receptors of the pancreas: a correlation between displacement of (3 H)-quinuclidinyl benzilate binding and amylase secretion. (3H)-quinuclidinyl benzilate [(3H)-QNB], A potent muscarinic antagonist, was used as a tool to reveal the presence of muscarinic receptors in rat pancreas. Binding assays were performed on a 49,000 g pellet. Known muscarinic antagonists and agonists were used for competition assays on (3N)-QNB binding and studies on amylase secretion in vitro. A correlation was established between the binding assays and the studies on amylase release which suggests that the binding sites and those involved in the cholinergic control of pancreatic enzyme secretion are similar."}
{"id": "PMID:482347", "title": "A study of small intestinal dipeptidases of rats: effect of vinblastine treatment.", "content": "Activities of L-glycyl-L-valine and L-glycyl-L-leucine dipeptidase were studied in the mucosa of the small intestine of rats following intraperitoneal administration of vinblastine sulfate (1.0 mg/kg). Activities of these dipeptidases were depressed significantly; the effect was maximal between 4 and 5 h after the injection and lasted for more than 16 h. The depression in the enzyme activities reached even up to 95% when the alkaloid was administered repeatedly in 4-hourly intervals. The present results, showing a dose-dependent inhibitory effect on these dipeptidases, suggest a toxic effect on the enzymes in addition to simple inhibition resulting from a blockade of cell division. The former possibility was examined by incubating mucosal homogenates with various concentrations of the alkaloid for 30 min. Subsequent measurement of the activities showed a significant depression when the alkaloid concentration was raised to 10 microgram/mg wet mucosa. It is concluded that vinblastine sulfate not only arrests the mitosis of the crypts but influences, additionally, the enzymatic complements of the enterocytes.", "contents": "A study of small intestinal dipeptidases of rats: effect of vinblastine treatment. Activities of L-glycyl-L-valine and L-glycyl-L-leucine dipeptidase were studied in the mucosa of the small intestine of rats following intraperitoneal administration of vinblastine sulfate (1.0 mg/kg). Activities of these dipeptidases were depressed significantly; the effect was maximal between 4 and 5 h after the injection and lasted for more than 16 h. The depression in the enzyme activities reached even up to 95% when the alkaloid was administered repeatedly in 4-hourly intervals. The present results, showing a dose-dependent inhibitory effect on these dipeptidases, suggest a toxic effect on the enzymes in addition to simple inhibition resulting from a blockade of cell division. The former possibility was examined by incubating mucosal homogenates with various concentrations of the alkaloid for 30 min. Subsequent measurement of the activities showed a significant depression when the alkaloid concentration was raised to 10 microgram/mg wet mucosa. It is concluded that vinblastine sulfate not only arrests the mitosis of the crypts but influences, additionally, the enzymatic complements of the enterocytes."}
{"id": "PMID:482348", "title": "Effects of piracetam on the incorporation of 32P into the phospholipids of neurons and glial cells isolated from rabbit cerebral cortex.", "content": "In the search for the biochemical basis of the action of Piracetam, the effects of this encephalotropic substance on the neuronal and glial phospholipid metabolism was investigated. Piracetam increases the incorporation of 32P into phosphatidylinositol and phosphatidyl choline of both glia and neuronal cell bodies (Figs. 1 and 2). When taking the important role of phosphatidylinositol in the processes of synaptic transmission and axonal conduction into consideration, the data obtained in the present work suggest that piracetam may stimulate excitatory neurons and may be involved in the process of synaptic transmission. The stimulatory effect of piracetam on the incorporation of 32P into phosphatidylinositol and phosphatidyl choline appears to be mediated by noerpinephrine or another neurotransmitter. Glial cells, isolated from the cerebral cortex of a rabbit, contained approximately one-third more phospholipids per uint protein than the neuronal cell bodies. The distribution and pattern of phospholipid relative to the total amount, was rather similar in both cell types. The incorporation of 32P into phosphatidylinositol and phosphatidyl choline was somewhat faster in neurons than in glial cells. Compared to glial cells the neuronal cell fraction had a higher phospholipid turnover.", "contents": "Effects of piracetam on the incorporation of 32P into the phospholipids of neurons and glial cells isolated from rabbit cerebral cortex. In the search for the biochemical basis of the action of Piracetam, the effects of this encephalotropic substance on the neuronal and glial phospholipid metabolism was investigated. Piracetam increases the incorporation of 32P into phosphatidylinositol and phosphatidyl choline of both glia and neuronal cell bodies (Figs. 1 and 2). When taking the important role of phosphatidylinositol in the processes of synaptic transmission and axonal conduction into consideration, the data obtained in the present work suggest that piracetam may stimulate excitatory neurons and may be involved in the process of synaptic transmission. The stimulatory effect of piracetam on the incorporation of 32P into phosphatidylinositol and phosphatidyl choline appears to be mediated by noerpinephrine or another neurotransmitter. Glial cells, isolated from the cerebral cortex of a rabbit, contained approximately one-third more phospholipids per uint protein than the neuronal cell bodies. The distribution and pattern of phospholipid relative to the total amount, was rather similar in both cell types. The incorporation of 32P into phosphatidylinositol and phosphatidyl choline was somewhat faster in neurons than in glial cells. Compared to glial cells the neuronal cell fraction had a higher phospholipid turnover."}
{"id": "PMID:482349", "title": "[Clinico-physiological sleep- and hangover-spectra from pentobarbital, promazin and their combination, as reflected by self-ratings of young and elderly subjects (author's transl)].", "content": "A sample of 8 young and 8 elderly subjects was examined in a 2x2x2 factorial design, whether and how promazine interacts with pentobarbital as a hypnotic agent. Analyses of self-reports led to the following conclusions: 1. Young subjects and not experience any significant effects from either the single components (pentobarbital, promazine) and/or their combination. 2. Elderly subjects experienced positive effects in sleep and hangover parameters under the single components as well as under the combination. Consequences for research in clincal psychopharmacology are discussed.", "contents": "[Clinico-physiological sleep- and hangover-spectra from pentobarbital, promazin and their combination, as reflected by self-ratings of young and elderly subjects (author's transl)]. A sample of 8 young and 8 elderly subjects was examined in a 2x2x2 factorial design, whether and how promazine interacts with pentobarbital as a hypnotic agent. Analyses of self-reports led to the following conclusions: 1. Young subjects and not experience any significant effects from either the single components (pentobarbital, promazine) and/or their combination. 2. Elderly subjects experienced positive effects in sleep and hangover parameters under the single components as well as under the combination. Consequences for research in clincal psychopharmacology are discussed."}
{"id": "PMID:482350", "title": "beta-Endorphin-like immunoreactivity in cerebrospinal fluid and plasma of patients with schizophrenia and other neuropsychiatric disorders.", "content": "Measurements of beta-endorphin-like immunoreactivity have been performed in CSF and plasma of patients with schizophrenia and other neuropsychiatric disorders. The detection limit of the RIA was between 20--50 pg/ml (6--15 fmole/ml). In CSF the quantity of beta-endorphin-like immunoreactivity ranges up to 65 pg/ml. The data from schizophrenics and other neuropsychiatric patients show no obvious deviation from the results in a control group of medical patients with normal CSF findings. In plasma the immunoreactive beta-endorphin-like material ranges up to 250 pg/ml. There is only a small tendency to higher values in schizophrenic patients, if compared with different types of neuroses and affective and organic psychoses. In a second series of experiments also this tendency could not be reproduced. In 9 electroconvulsive treatments an increase of blood beta-endorphin-like immunoreactivity was observed 7 times. A possible endorphinergic mechanism in the mode of action of electroconvulsion is hypothesized.", "contents": "beta-Endorphin-like immunoreactivity in cerebrospinal fluid and plasma of patients with schizophrenia and other neuropsychiatric disorders. Measurements of beta-endorphin-like immunoreactivity have been performed in CSF and plasma of patients with schizophrenia and other neuropsychiatric disorders. The detection limit of the RIA was between 20--50 pg/ml (6--15 fmole/ml). In CSF the quantity of beta-endorphin-like immunoreactivity ranges up to 65 pg/ml. The data from schizophrenics and other neuropsychiatric patients show no obvious deviation from the results in a control group of medical patients with normal CSF findings. In plasma the immunoreactive beta-endorphin-like material ranges up to 250 pg/ml. There is only a small tendency to higher values in schizophrenic patients, if compared with different types of neuroses and affective and organic psychoses. In a second series of experiments also this tendency could not be reproduced. In 9 electroconvulsive treatments an increase of blood beta-endorphin-like immunoreactivity was observed 7 times. A possible endorphinergic mechanism in the mode of action of electroconvulsion is hypothesized."}
{"id": "PMID:482351", "title": "Effects of the synthetic analogue of methionine enkephalin FK 33-824 on psychotic symptoms.", "content": "In an open pilot study, 9 schizophrenic patients were treated with the synthetic analogue of methionin-enkephalin FK 33-824 on two consecutive days in a dose of 0.5 mg on the first day, and 1.0 mg on the second day (infusion for 2 hours). 3 patients refused therapy during or after the first infusion; of the remaining 6 patients (2 hebephrenic, 4 paranoid type) 5 patients improved remarkably on the first and second day. The total BPRS-score and 4 of the 5 factors of the BPRS-scale were reduced significantly the day after treatment compared to pretreatment values. The improvement continued for 28-168 hours.", "contents": "Effects of the synthetic analogue of methionine enkephalin FK 33-824 on psychotic symptoms. In an open pilot study, 9 schizophrenic patients were treated with the synthetic analogue of methionin-enkephalin FK 33-824 on two consecutive days in a dose of 0.5 mg on the first day, and 1.0 mg on the second day (infusion for 2 hours). 3 patients refused therapy during or after the first infusion; of the remaining 6 patients (2 hebephrenic, 4 paranoid type) 5 patients improved remarkably on the first and second day. The total BPRS-score and 4 of the 5 factors of the BPRS-scale were reduced significantly the day after treatment compared to pretreatment values. The improvement continued for 28-168 hours."}
{"id": "PMID:482362", "title": "[Muscular compartment leg syndrome].", "content": "Certain forms of claudication are due to excess tissue pressure of the muscles of the aponeurotic chambers of the leg. These have been well described by Reneman, especially in their chronic form and for the anterior and lateral chambers. The claudication is due to an overworking of the muscles caused by walking, running or effort. It has its own characteristics, with direct measurement of the tissue pressure and phlebography aiding the diagnosis and attention being drawn by the presence of a \"muscular hernia of the leg\". Fasciotomy is the corrective treatment for cases of hyperalgia and beginning with a certain gradient of muscle pressure. The pathogenic reasons for this are discussed.", "contents": "[Muscular compartment leg syndrome]. Certain forms of claudication are due to excess tissue pressure of the muscles of the aponeurotic chambers of the leg. These have been well described by Reneman, especially in their chronic form and for the anterior and lateral chambers. The claudication is due to an overworking of the muscles caused by walking, running or effort. It has its own characteristics, with direct measurement of the tissue pressure and phlebography aiding the diagnosis and attention being drawn by the presence of a \"muscular hernia of the leg\". Fasciotomy is the corrective treatment for cases of hyperalgia and beginning with a certain gradient of muscle pressure. The pathogenic reasons for this are discussed."}
{"id": "PMID:482361", "title": "[Tissue pressure of the leg].", "content": "The underlying concepts of the study of subcutaneous and intramuscular tissue pressure, which began in 1952, have been highly modified. Three types of methods are now used: direct needle puncture, the perforated capsule, and the microcatheter. The capsule and the catheter are sensitive to osmotic and hydrostatic tissue pressure, and the needle indicates an interstitial hydrostatic T.P. We prefer the latter method, which allows the total T.P. to be measured.", "contents": "[Tissue pressure of the leg]. The underlying concepts of the study of subcutaneous and intramuscular tissue pressure, which began in 1952, have been highly modified. Three types of methods are now used: direct needle puncture, the perforated capsule, and the microcatheter. The capsule and the catheter are sensitive to osmotic and hydrostatic tissue pressure, and the needle indicates an interstitial hydrostatic T.P. We prefer the latter method, which allows the total T.P. to be measured."}
{"id": "PMID:482364", "title": "[Compression technic of the upper limbs with adhesive bands to reduce edema].", "content": "Whatever the cause of an edema of the lower limbs, compression with temporary or permanent adhesive bands proves efficient. The wide range of bands proposed by manufacturers, makes it possible to find compressions that are well-suited to the case to be treated. The band should be rolled from the end to the root of the limbs, protect the skin beforehand with cotton and talcum. The deltoidian area is molded by cutting pieces of the band. The hand is also wrapped, with the fingers left free. The difficulty resides in the pressure to be applied: it is preferable to proceed in a gradual manner, beginning with a slight pressure. The bandage should be changed once or twice a week while the edema is being reduced, but may be left in place for six weeks.", "contents": "[Compression technic of the upper limbs with adhesive bands to reduce edema]. Whatever the cause of an edema of the lower limbs, compression with temporary or permanent adhesive bands proves efficient. The wide range of bands proposed by manufacturers, makes it possible to find compressions that are well-suited to the case to be treated. The band should be rolled from the end to the root of the limbs, protect the skin beforehand with cotton and talcum. The deltoidian area is molded by cutting pieces of the band. The hand is also wrapped, with the fingers left free. The difficulty resides in the pressure to be applied: it is preferable to proceed in a gradual manner, beginning with a slight pressure. The bandage should be changed once or twice a week while the edema is being reduced, but may be left in place for six weeks."}
{"id": "PMID:482372", "title": "Temporal aspect os articulatory movement for /s/-stop clusters.", "content": "Cinefluorographic tracking of pellets on the tongue body, tongue tip, jaw, and lips of three subjects indicates that there are certain basic strategies used for initial /sp/, /st/ and /sk/ clusters. The data reveal an economy of effort in tongue movement, and support the concept of the tongue as a multiple articulator. In addition, the shorter fricative duration of /s/ before /p/ is seen to be a result of early lip closure relative to the occlusions for /st/ and /sk/, which are delayed due to tongue involvement with the /s/.", "contents": "Temporal aspect os articulatory movement for /s/-stop clusters. Cinefluorographic tracking of pellets on the tongue body, tongue tip, jaw, and lips of three subjects indicates that there are certain basic strategies used for initial /sp/, /st/ and /sk/ clusters. The data reveal an economy of effort in tongue movement, and support the concept of the tongue as a multiple articulator. In addition, the shorter fricative duration of /s/ before /p/ is seen to be a result of early lip closure relative to the occlusions for /st/ and /sk/, which are delayed due to tongue involvement with the /s/."}
{"id": "PMID:482368", "title": "[Proof of the effectiveness of compression by nuclear medicine, plethysmography and venous pressure measurement].", "content": "1) In a lying down position a) immediately and under rapid compression, one observes a reduction in muscle flow as well as a reduction of the perfusion of the foot and of the systolic pressure of the big toe. After disinfiltration of the leg by compression, the muscular flow more than doubles (clearance with Xenon 133). b) a reduction in the venous blood volume of the limb by about 1/3. c) an increase in the venous circulation speed by about 1.5 times (tracer in a vein of the back of the foot). The degree of change is depending from the pressure of the compressive bandage (in a lying down position, between 20 mmHg and a maximum of 40 mmHg). 2) while walking a) shortening of the pathological refluxes in the veins of the lower limbs (directional Doppler). b) improvement of the pump output (increase inthe volume of blood pumped upwards), and a variable influence of the peripheral venous pressure (simultaneous methods of plethysmography of the foot and the venous pressure). c) reduction of edema, improvement of the disturbed lymphatic flow (plethysmography, lymphotropic tracers with radioactive marking).", "contents": "[Proof of the effectiveness of compression by nuclear medicine, plethysmography and venous pressure measurement]. 1) In a lying down position a) immediately and under rapid compression, one observes a reduction in muscle flow as well as a reduction of the perfusion of the foot and of the systolic pressure of the big toe. After disinfiltration of the leg by compression, the muscular flow more than doubles (clearance with Xenon 133). b) a reduction in the venous blood volume of the limb by about 1/3. c) an increase in the venous circulation speed by about 1.5 times (tracer in a vein of the back of the foot). The degree of change is depending from the pressure of the compressive bandage (in a lying down position, between 20 mmHg and a maximum of 40 mmHg). 2) while walking a) shortening of the pathological refluxes in the veins of the lower limbs (directional Doppler). b) improvement of the pump output (increase inthe volume of blood pumped upwards), and a variable influence of the peripheral venous pressure (simultaneous methods of plethysmography of the foot and the venous pressure). c) reduction of edema, improvement of the disturbed lymphatic flow (plethysmography, lymphotropic tracers with radioactive marking)."}
{"id": "PMID:482369", "title": "[Physiology of venous circulation].", "content": "The venous system becomes deformed and distended under the effect of neighboring tissues and hydrostatic pressure. It is thus under a constant threat of stasis. While lying-down at rest, the venous blood is propulsed by means of the pressure gradient maintained by the vis a tergo and the vis a fronte, modulated by the parietal tonus. While standing and stationary, decompensation occurs despite the bulbar reflexes, despite lymphatic resorption and despite the counter-pressure of the perivenous tissues. However, muscular activity and especially walking at a steady pace, restores the resorption of the interstitial liquids, tanks particularly to the musculovascular synergy or the peripheral heart.", "contents": "[Physiology of venous circulation]. The venous system becomes deformed and distended under the effect of neighboring tissues and hydrostatic pressure. It is thus under a constant threat of stasis. While lying-down at rest, the venous blood is propulsed by means of the pressure gradient maintained by the vis a tergo and the vis a fronte, modulated by the parietal tonus. While standing and stationary, decompensation occurs despite the bulbar reflexes, despite lymphatic resorption and despite the counter-pressure of the perivenous tissues. However, muscular activity and especially walking at a steady pace, restores the resorption of the interstitial liquids, tanks particularly to the musculovascular synergy or the peripheral heart."}
{"id": "PMID:482373", "title": "Intrusive stops in nasal-fricative clusters: an aerodynamic and acoustic investigation.", "content": "An aerodynamic investigation of the occurrence of intrusive stop consonants occurring in nasal-fricative consonant clusters in English revealed that intrusive stops were cued perceptually by silent gaps often followed by burst releases preceding the fricative consonant. In articulatory-aerodynamic terms, intrusive stops appear to result from a prolonged oral occlusion of the nasal stop which is released with a vigorous burst release just prior to complete formation of the following fricative slit constriction.", "contents": "Intrusive stops in nasal-fricative clusters: an aerodynamic and acoustic investigation. An aerodynamic investigation of the occurrence of intrusive stop consonants occurring in nasal-fricative consonant clusters in English revealed that intrusive stops were cued perceptually by silent gaps often followed by burst releases preceding the fricative consonant. In articulatory-aerodynamic terms, intrusive stops appear to result from a prolonged oral occlusion of the nasal stop which is released with a vigorous burst release just prior to complete formation of the following fricative slit constriction."}
{"id": "PMID:482374", "title": "Breathing, pausing and reading.", "content": "An analysis of the breathing patterns of speakers in a variable-rate reading task shows that the duration and frequency of breathing pauses are dependent both on the rate of speaking and the syntactic nature of the pause location. Non-breathing pauses follow the same pattern of occurrence as breathing pauses, but are always shorter and tend to occur primarily at minor constituent breaks. At slow and normal rates, speakers accommodate their need to inhale to the preplanned pause patterns. At fast rates, however, the physiological need to breathe is the sole determinant of pausing.", "contents": "Breathing, pausing and reading. An analysis of the breathing patterns of speakers in a variable-rate reading task shows that the duration and frequency of breathing pauses are dependent both on the rate of speaking and the syntactic nature of the pause location. Non-breathing pauses follow the same pattern of occurrence as breathing pauses, but are always shorter and tend to occur primarily at minor constituent breaks. At slow and normal rates, speakers accommodate their need to inhale to the preplanned pause patterns. At fast rates, however, the physiological need to breathe is the sole determinant of pausing."}
{"id": "PMID:482370", "title": "[Venous physiopathology. The major pathogenic processes in venous pathology and their consequences].", "content": "Various pathological circumstances, and especially: acute venous thrombosis, superficial venous insufficiency and post-phlebitic disease, may upset the conditions of venous circumlation. Among the major pathological processes that may thus be involved, one can underline: -- Venous obstruction, whose physiopathology is different in the acute thrombosis and in the post-phlebitic phase. Especially studied in this work are: the phases of the enry into the acute venous stasis, which may lead to venous gangrene -- the offset and non offset obstructive syndromes. The offsetting of a venous obstruction depending on the development of a sufficient and effective substitute circulation. -- The orthostatic reflux may involve the superficial venous network (superficial venous insufficiency) or the deep one (deep venous insufficiency). In either case, the insufficiency of the communicating veins may complicate the primary venous insufficiency. -- On the tissue level, various pathogenic processes induce a peripheral venous stasis, generating problems that may be classed under three headings: lymphatic repercussions, arterial problems and dysfunctions of the arterio-venous anastomoses. These various processes and their consequences on the tissue level, are studied.", "contents": "[Venous physiopathology. The major pathogenic processes in venous pathology and their consequences]. Various pathological circumstances, and especially: acute venous thrombosis, superficial venous insufficiency and post-phlebitic disease, may upset the conditions of venous circumlation. Among the major pathological processes that may thus be involved, one can underline: -- Venous obstruction, whose physiopathology is different in the acute thrombosis and in the post-phlebitic phase. Especially studied in this work are: the phases of the enry into the acute venous stasis, which may lead to venous gangrene -- the offset and non offset obstructive syndromes. The offsetting of a venous obstruction depending on the development of a sufficient and effective substitute circulation. -- The orthostatic reflux may involve the superficial venous network (superficial venous insufficiency) or the deep one (deep venous insufficiency). In either case, the insufficiency of the communicating veins may complicate the primary venous insufficiency. -- On the tissue level, various pathogenic processes induce a peripheral venous stasis, generating problems that may be classed under three headings: lymphatic repercussions, arterial problems and dysfunctions of the arterio-venous anastomoses. These various processes and their consequences on the tissue level, are studied."}
{"id": "PMID:482371", "title": "[Complications of sclerotherapy].", "content": "The authors recently treated three patients showing rather marked complications following sclerosing injections for varicose veins. In the first case, the intra-arterial injection brought about a tissular necrosis in the form of a distal-based triangle. The preservative treatment, undertaken 4 weeks after the injection, did not make it possible to save three toes, that had to be amputated. The second patient was sent to use after an injection in the posterior tibial artery. Acute ischemia was treated on an emergency basis with a lumbar sympathectomy. I believe that the approach we took allowed us to cure the trophic problems and to loose only one small phalanx. The third case reported on concerns a patient brought to us in a state of shock after a massive pulmonary embolism. She had been given a sclerosing injection in a large varicose vein of the leg 48 hours previously. The leg had rapidly increased in volume and was apparently the site of a deep veinous thrombosis.", "contents": "[Complications of sclerotherapy]. The authors recently treated three patients showing rather marked complications following sclerosing injections for varicose veins. In the first case, the intra-arterial injection brought about a tissular necrosis in the form of a distal-based triangle. The preservative treatment, undertaken 4 weeks after the injection, did not make it possible to save three toes, that had to be amputated. The second patient was sent to use after an injection in the posterior tibial artery. Acute ischemia was treated on an emergency basis with a lumbar sympathectomy. I believe that the approach we took allowed us to cure the trophic problems and to loose only one small phalanx. The third case reported on concerns a patient brought to us in a state of shock after a massive pulmonary embolism. She had been given a sclerosing injection in a large varicose vein of the leg 48 hours previously. The leg had rapidly increased in volume and was apparently the site of a deep veinous thrombosis."}
{"id": "PMID:482383", "title": "Studies on PGBx, a polymeric derivative of prostaglandin B1: I. Synthesis and purification of PGBx.", "content": "PGBx, a new polymeric derivative of PGB1, previously was shown to (a) restore oxidative phosphorylation to degraded isolated rat liver mitochondria in vitro and (b) to reverse the effects of cardiogenic ischemia in monkeys and cerebral ischemia in rabbits. This report describes in detail the synthesis and purification of PGBx via PGB1, starting with azelaic acid. Details of the in vitro mitochondrial assay are also reported. Purified PGBx exhibiting maximal reactivation of mitochondrial phosphorylation has a mean molecular weight of 2350. Yield of PGBx based on azelaic acid is 4% and based on PGB1 is 25%.", "contents": "Studies on PGBx, a polymeric derivative of prostaglandin B1: I. Synthesis and purification of PGBx. PGBx, a new polymeric derivative of PGB1, previously was shown to (a) restore oxidative phosphorylation to degraded isolated rat liver mitochondria in vitro and (b) to reverse the effects of cardiogenic ischemia in monkeys and cerebral ischemia in rabbits. This report describes in detail the synthesis and purification of PGBx via PGB1, starting with azelaic acid. Details of the in vitro mitochondrial assay are also reported. Purified PGBx exhibiting maximal reactivation of mitochondrial phosphorylation has a mean molecular weight of 2350. Yield of PGBx based on azelaic acid is 4% and based on PGB1 is 25%."}
{"id": "PMID:482384", "title": "PMR studies of phosphatidylcholine-cholesterol vesicles interacting with lucensomycin.", "content": "Spin-lattice relaxation times (T1) were measured above the phase transition temperature on sonicated vesicles of egg- or dipalmitoyl-phosphatidylcholine containing cholesterol and/or the polyenic antibiotic, lucensomycin. T1 values of only the terminal methyl groups of the fatty acyl chains were significantly reduced by cholesterol. Lucensomycin caused, more markedly in cholesterol-containing vesicles, a selective reduction of the T1 values of the N-methyl groups. An even more conspicuous decrease, occurring only in cholesterol-containing vesicles, was observed for the transverse relaxation times (T2) of the N-methyl signals upon addition of lucensomycin. The polyene failed to remove the well-known broadening effect of cholesterol on phosphatidylcholine methylene signals. These results indicate that as lucensomycin binds to cholesterol-containing membranes, there is a detectable perturbation of the dynamic structure of the N-methyl groups with an increase in the degree of motional anisotropy. But the non-polar region of the bilayer seems not significantly perturbed by the polyene.", "contents": "PMR studies of phosphatidylcholine-cholesterol vesicles interacting with lucensomycin. Spin-lattice relaxation times (T1) were measured above the phase transition temperature on sonicated vesicles of egg- or dipalmitoyl-phosphatidylcholine containing cholesterol and/or the polyenic antibiotic, lucensomycin. T1 values of only the terminal methyl groups of the fatty acyl chains were significantly reduced by cholesterol. Lucensomycin caused, more markedly in cholesterol-containing vesicles, a selective reduction of the T1 values of the N-methyl groups. An even more conspicuous decrease, occurring only in cholesterol-containing vesicles, was observed for the transverse relaxation times (T2) of the N-methyl signals upon addition of lucensomycin. The polyene failed to remove the well-known broadening effect of cholesterol on phosphatidylcholine methylene signals. These results indicate that as lucensomycin binds to cholesterol-containing membranes, there is a detectable perturbation of the dynamic structure of the N-methyl groups with an increase in the degree of motional anisotropy. But the non-polar region of the bilayer seems not significantly perturbed by the polyene."}
{"id": "PMID:482385", "title": "The importance of liver in normal and silicotic lung-lipid homeostasis: 3. Triacylglycerols.", "content": "Early lung silicosis in rats activates the liver for triacylglycerol (TG) biosynthesis. The newly formed liver TG seems mobilized to the silicotic lung, as indicated by increased lung TG content and specific activity. These TG changes are associated with decreased TG content and increased TG specific activity in the liver. Serum TGs are also decreased at the study times of 6, 24, 72, and 144 h post intrapulmonary silica treatment.", "contents": "The importance of liver in normal and silicotic lung-lipid homeostasis: 3. Triacylglycerols. Early lung silicosis in rats activates the liver for triacylglycerol (TG) biosynthesis. The newly formed liver TG seems mobilized to the silicotic lung, as indicated by increased lung TG content and specific activity. These TG changes are associated with decreased TG content and increased TG specific activity in the liver. Serum TGs are also decreased at the study times of 6, 24, 72, and 144 h post intrapulmonary silica treatment."}
{"id": "PMID:482386", "title": "Pulsed nuclear magnetic resonance of potassium (39K) of whole body live and dead newborn mice. Double oscillation frequencies in T1 decay curves.", "content": "Pulsed nuclear magnetic resonance relaxation curves (T2 and T1) of potassium (39K) have been measured in detail on whole body newborn mice when alive, and on the same mice after death. The T2 curves are simple exponential with respect to time, but are shorter than for 39K in simple solutions. The T1 curves are not exponential decays, but show large oscillations that may be described approximately as the sum of two separate sine waves of different frequencies. Large T1 oscillations of complex waveform were previously observed by us with 39K in cancer tissues. Gyroscopic motion of adsorbed magnetoelectric dipoles is proposed as a possible physical mechanism accounting for the experimental observations.", "contents": "Pulsed nuclear magnetic resonance of potassium (39K) of whole body live and dead newborn mice. Double oscillation frequencies in T1 decay curves. Pulsed nuclear magnetic resonance relaxation curves (T2 and T1) of potassium (39K) have been measured in detail on whole body newborn mice when alive, and on the same mice after death. The T2 curves are simple exponential with respect to time, but are shorter than for 39K in simple solutions. The T1 curves are not exponential decays, but show large oscillations that may be described approximately as the sum of two separate sine waves of different frequencies. Large T1 oscillations of complex waveform were previously observed by us with 39K in cancer tissues. Gyroscopic motion of adsorbed magnetoelectric dipoles is proposed as a possible physical mechanism accounting for the experimental observations."}
{"id": "PMID:482387", "title": "Influence of superoxide generating system, vitamin E, and superoxide dismutase on radiation consequences.", "content": "During studies of the mechanism by which hemolysis is induced in irradiated human erythrocytes in vitro, several inducements of membrane lipid peroxidation and protective effects of vitamin E (V.E) and superoxide dismutase (SOD) were investigated. Findings were: (1) Before hemolysis, K+ release from erythrocytes induced by radiation stimulated hemolysis but was inhibited by V.E or SOD. (2) Lipid peroxidation of mitochondria induced by Fe3+, ADP, and superoxide (O2-) generating system, and lipid peroxidation of microsome induced by O2- generating system, were also inhibited by V.E or SOD. (3) X-ray or 60Co gamma-ray radiation stimulated lipid peroxidation of liver homogenate, microsome, and liposome. Some of this peroxidation was inhibited by V.E. or SOD. These results suggest that O2- and/or OH formation by radiation induces membrane lipid peroxidation, which causes deterioration of membrane resulting in change of ion permeability and consequent hemolysis.", "contents": "Influence of superoxide generating system, vitamin E, and superoxide dismutase on radiation consequences. During studies of the mechanism by which hemolysis is induced in irradiated human erythrocytes in vitro, several inducements of membrane lipid peroxidation and protective effects of vitamin E (V.E) and superoxide dismutase (SOD) were investigated. Findings were: (1) Before hemolysis, K+ release from erythrocytes induced by radiation stimulated hemolysis but was inhibited by V.E or SOD. (2) Lipid peroxidation of mitochondria induced by Fe3+, ADP, and superoxide (O2-) generating system, and lipid peroxidation of microsome induced by O2- generating system, were also inhibited by V.E or SOD. (3) X-ray or 60Co gamma-ray radiation stimulated lipid peroxidation of liver homogenate, microsome, and liposome. Some of this peroxidation was inhibited by V.E. or SOD. These results suggest that O2- and/or OH formation by radiation induces membrane lipid peroxidation, which causes deterioration of membrane resulting in change of ion permeability and consequent hemolysis."}
{"id": "PMID:482388", "title": "Stabilizing effects of cholesterol on changes in membrane permeability and potential induced in red blood cells by lysolecithin.", "content": "Effects of cholesterol on permeability of K+ ion and on change in membrane potential induced by lysolecithin were studied. Cholesterol inhibited K+ release from rabbit red blood cells treated with lysolecithin (1.25 micrograms/ml), 3.3 X 10(-6) M of cholesterol being the optimum concentration for blocking K+ release. Changes in membrane potential, monitored by changes in intensity of fluorescence of cyanine dye, were induced by lysolecithin and inhibited by cholesterol. The inhibitory action on both K+ permeability and membrane potential varied with the cholesterol concentration. The observed effects are thought to be due to membrane-stabilizing activities such as decreasing membrane fluidity and hardening the membrane at the fluid-phase transition temperature. These properties of cholesterol may have significance in relation to transformed cells (tumor cells, lymphomed cells).", "contents": "Stabilizing effects of cholesterol on changes in membrane permeability and potential induced in red blood cells by lysolecithin. Effects of cholesterol on permeability of K+ ion and on change in membrane potential induced by lysolecithin were studied. Cholesterol inhibited K+ release from rabbit red blood cells treated with lysolecithin (1.25 micrograms/ml), 3.3 X 10(-6) M of cholesterol being the optimum concentration for blocking K+ release. Changes in membrane potential, monitored by changes in intensity of fluorescence of cyanine dye, were induced by lysolecithin and inhibited by cholesterol. The inhibitory action on both K+ permeability and membrane potential varied with the cholesterol concentration. The observed effects are thought to be due to membrane-stabilizing activities such as decreasing membrane fluidity and hardening the membrane at the fluid-phase transition temperature. These properties of cholesterol may have significance in relation to transformed cells (tumor cells, lymphomed cells)."}
{"id": "PMID:482389", "title": "Electron microscopic examination of common red cell ghosts: cytoplasmic contamination.", "content": "Red blood cell ghost preparations are often cited as providing unequivocal or convincing evidence for the active transport of solutes from a solution of low concentration across a membrane to a solution of higher concentration. Electron microscopic examination of the more widely used ghost preparations show that a considerable quantity of cytoplasmic macromolecules (including hemoglobin) remain within the treated red blood cells. That is, many of the ghost preparations are not hollow membrane perparations. It is concluded that the problem of active solute transport in red blood cell ghost preparations should be reexamined. Furthermore, experiments with ghost preparations purporting to demonstrate active transport should include electron photomicrographs of the preparation utilized.", "contents": "Electron microscopic examination of common red cell ghosts: cytoplasmic contamination. Red blood cell ghost preparations are often cited as providing unequivocal or convincing evidence for the active transport of solutes from a solution of low concentration across a membrane to a solution of higher concentration. Electron microscopic examination of the more widely used ghost preparations show that a considerable quantity of cytoplasmic macromolecules (including hemoglobin) remain within the treated red blood cells. That is, many of the ghost preparations are not hollow membrane perparations. It is concluded that the problem of active solute transport in red blood cell ghost preparations should be reexamined. Furthermore, experiments with ghost preparations purporting to demonstrate active transport should include electron photomicrographs of the preparation utilized."}
{"id": "PMID:482391", "title": "Transaminative pathway of cysteine metabolism in rat tissues.", "content": "Enzyme activities of the transaminative pathway of cysteine metabolism in various rat tissues were examined. Liver was found the most active tissue, followed by kidney and heart. Liver and kidney were more pronounced in mercaptopyruvate sulfurtransferase activity than in cysteine transaminase activity; heart was more active in the latter. Red blood cells, which have pronounced sulfurtransferase activity, exhibited no transaminase activity, indicating the pathway is negligible in this tissue.", "contents": "Transaminative pathway of cysteine metabolism in rat tissues. Enzyme activities of the transaminative pathway of cysteine metabolism in various rat tissues were examined. Liver was found the most active tissue, followed by kidney and heart. Liver and kidney were more pronounced in mercaptopyruvate sulfurtransferase activity than in cysteine transaminase activity; heart was more active in the latter. Red blood cells, which have pronounced sulfurtransferase activity, exhibited no transaminase activity, indicating the pathway is negligible in this tissue."}
{"id": "PMID:482392", "title": "The role of inert particles in malignant transformations: a hypothesis of carcinogenesis.", "content": "Controlled cellular growth exists in an aqueous matrix that undergoes a spectrum of reversible solvent and structural conformational changes. In contrast, malignant tumor formation occurs in an irreversible nonpolar aqueous matrix (coacervate); that is, in a physicochemical sense uncontrollable cell growth exists in a hydrocarbon-like milieu. It is proposed that foreign isoluble particles which locate on cellular surfaces can induce such coacervate structuring. Resultant formation of abnormal film matrices could initiate pathological transformations in the morphology, metabolism, and replication of the affected cells. In short, this abnormal matrix formation may be a critical factor in the development of malignancies, both avascular and vascular stages. It would follow that drugs or other agents capable of disrupting coacervated water matrices could be effective in the treatment of cancer. Experimental studies in our laboratory and clinical data from other sources support this hypothesis.", "contents": "The role of inert particles in malignant transformations: a hypothesis of carcinogenesis. Controlled cellular growth exists in an aqueous matrix that undergoes a spectrum of reversible solvent and structural conformational changes. In contrast, malignant tumor formation occurs in an irreversible nonpolar aqueous matrix (coacervate); that is, in a physicochemical sense uncontrollable cell growth exists in a hydrocarbon-like milieu. It is proposed that foreign isoluble particles which locate on cellular surfaces can induce such coacervate structuring. Resultant formation of abnormal film matrices could initiate pathological transformations in the morphology, metabolism, and replication of the affected cells. In short, this abnormal matrix formation may be a critical factor in the development of malignancies, both avascular and vascular stages. It would follow that drugs or other agents capable of disrupting coacervated water matrices could be effective in the treatment of cancer. Experimental studies in our laboratory and clinical data from other sources support this hypothesis."}
{"id": "PMID:482433", "title": "The rhomboid-to-W technique for excision of some skin lesions and closure.", "content": "A method is descirbed whereby a skin lesion is excised as a rhomboid, and adjacent flaps are then created and transposed to close the defect in a W. A. pleasing scar line results, one which is easily hidden in the natural creases.", "contents": "The rhomboid-to-W technique for excision of some skin lesions and closure. A method is descirbed whereby a skin lesion is excised as a rhomboid, and adjacent flaps are then created and transposed to close the defect in a W. A. pleasing scar line results, one which is easily hidden in the natural creases."}
{"id": "PMID:482434", "title": "Modified three-wall orbital expansion to correct persistent exophthalmos or exorbitism.", "content": "It is believed that a variation of the 3-wall orbital expansion of Tessier can be used to correct persistent exophthalmos, as well as exorbitism. The operation is described, and the results in 3 patients are shown.", "contents": "Modified three-wall orbital expansion to correct persistent exophthalmos or exorbitism. It is believed that a variation of the 3-wall orbital expansion of Tessier can be used to correct persistent exophthalmos, as well as exorbitism. The operation is described, and the results in 3 patients are shown."}
{"id": "PMID:482436", "title": "Anatomy of the mandibular branches of the facial nerve.", "content": "In operative dissections of mandibular branches of the facial nerve, we identified certain branches below the inferior border of the mandible in all cases. These usually supplied the depressor labii inferioris and mentalis muscles, though infrequently the branch to the depressor anguli oris was also below the mandible. At least 3 nerve branches were identified in all dissections. The clinical applications of this include the necessity to identify and protect these nerve branches during operations in the submandibular triangle, as well as when incising the platysma muscle or removing fat from over the body of the mandible in a face-lift procedure.", "contents": "Anatomy of the mandibular branches of the facial nerve. In operative dissections of mandibular branches of the facial nerve, we identified certain branches below the inferior border of the mandible in all cases. These usually supplied the depressor labii inferioris and mentalis muscles, though infrequently the branch to the depressor anguli oris was also below the mandible. At least 3 nerve branches were identified in all dissections. The clinical applications of this include the necessity to identify and protect these nerve branches during operations in the submandibular triangle, as well as when incising the platysma muscle or removing fat from over the body of the mandible in a face-lift procedure."}
{"id": "PMID:482437", "title": "Changing perspectives in surgical hand rehabilitation in quadriplegic patients.", "content": "Every motivated quadriplegic patient with resources for strong wrist extension, and lacking irreversible pathological features, is a candidate for reconstruction of at least a basic strong pinch. This basic pinch is better than that obtained with a prosthesis. We feel that this possibility exists in no less than 75 percent of our quadriplegic patients.", "contents": "Changing perspectives in surgical hand rehabilitation in quadriplegic patients. Every motivated quadriplegic patient with resources for strong wrist extension, and lacking irreversible pathological features, is a candidate for reconstruction of at least a basic strong pinch. This basic pinch is better than that obtained with a prosthesis. We feel that this possibility exists in no less than 75 percent of our quadriplegic patients."}
{"id": "PMID:482438", "title": "Arm dermolipectomy with a quadrangular flap and \"T\" closure.", "content": "We present a technique for the correction of obesity and/or ptosis of the arms. It consists of raising a lower quadrangular advancement flap, sliding the upper wound margin downward, cutting the excess off the quadrangular flap, and a T-shaped closure. A compressive elastic bandage is worn continuously for 3 months to help keep the closure scar flat and prevent its hypertrophy.", "contents": "Arm dermolipectomy with a quadrangular flap and \"T\" closure. We present a technique for the correction of obesity and/or ptosis of the arms. It consists of raising a lower quadrangular advancement flap, sliding the upper wound margin downward, cutting the excess off the quadrangular flap, and a T-shaped closure. A compressive elastic bandage is worn continuously for 3 months to help keep the closure scar flat and prevent its hypertrophy."}
{"id": "PMID:482439", "title": "Experimental study of results of nerve suture under tension vs. nerve grafting.", "content": "Evoked electromyograms and myelinated fiber caliber histograms were used to compare the results of end-to-end nerve suture and nerve grafting, performed with an operating microscope. The best results were achieved after direct suture without tension. Direct suture under a tension of 25 grams produced results sometimes comparable the that of the zero tension group, but mostly quite inferior to those and even to the grafted group. The regeneration of axons in the nerve graft group was delayed more than in the sutured groups, but by the tenth month the number of regenerating fibers had increased with stable and comparatively good results. After a nerve suture under a tension of 25 grams, there is impairment of the blood flow at the suture site, and the results are not as good. In such situations I believe nerve grafting should be performed. This amount of tension also seems to be the limit which will allow accurate coaptation of a nerve by microsurgical techniques.", "contents": "Experimental study of results of nerve suture under tension vs. nerve grafting. Evoked electromyograms and myelinated fiber caliber histograms were used to compare the results of end-to-end nerve suture and nerve grafting, performed with an operating microscope. The best results were achieved after direct suture without tension. Direct suture under a tension of 25 grams produced results sometimes comparable the that of the zero tension group, but mostly quite inferior to those and even to the grafted group. The regeneration of axons in the nerve graft group was delayed more than in the sutured groups, but by the tenth month the number of regenerating fibers had increased with stable and comparatively good results. After a nerve suture under a tension of 25 grams, there is impairment of the blood flow at the suture site, and the results are not as good. In such situations I believe nerve grafting should be performed. This amount of tension also seems to be the limit which will allow accurate coaptation of a nerve by microsurgical techniques."}
{"id": "PMID:482443", "title": "Early rotation of a cross-lip flap to repair a defect caused by a human bite.", "content": "A case is presented of loss of half of the lower lip from a human bite. An Estlander flap was used on the fifth day for reconstruction, with no infection and a good result. This shows the advantage of a \"delayed primary closure\" in some of these badly contaminated wounds.", "contents": "Early rotation of a cross-lip flap to repair a defect caused by a human bite. A case is presented of loss of half of the lower lip from a human bite. An Estlander flap was used on the fifth day for reconstruction, with no infection and a good result. This shows the advantage of a \"delayed primary closure\" in some of these badly contaminated wounds."}
{"id": "PMID:482444", "title": "Iatrogenic false aneurysms following punch hair grafting.", "content": "By serial angiograms, we found the pulsating nodules occasionally seen after punch hair grafting are pseudoaneurysms.", "contents": "Iatrogenic false aneurysms following punch hair grafting. By serial angiograms, we found the pulsating nodules occasionally seen after punch hair grafting are pseudoaneurysms."}
{"id": "PMID:482445", "title": "Improved method of injecting local anesthetic solutions for breast operations.", "content": "An improved method of injecting local anesthetic solutions for gynecomastia operations and augmentation mammaplasties is described.", "contents": "Improved method of injecting local anesthetic solutions for breast operations. An improved method of injecting local anesthetic solutions for gynecomastia operations and augmentation mammaplasties is described."}
{"id": "PMID:482448", "title": "[Angiitis of the CNS (and the problem of its classification)].", "content": "The author deals with the different and partially divergent aspects of the classification of inflammatory vascular processes with particular reference to cerebrospinal involvement and forms. The limited pathomorphic tissue reaction of vessel wall elements and blood cells to different etiopathogenetic factors and the different cellular tissue structures observed for similar noxae are especially worth mentioning in this context. Also pointed out by the author in his present paper are the limitations of diagnosis. Central-nervous-system involvement usually brings with it special topographic difficulties and problems.", "contents": "[Angiitis of the CNS (and the problem of its classification)]. The author deals with the different and partially divergent aspects of the classification of inflammatory vascular processes with particular reference to cerebrospinal involvement and forms. The limited pathomorphic tissue reaction of vessel wall elements and blood cells to different etiopathogenetic factors and the different cellular tissue structures observed for similar noxae are especially worth mentioning in this context. Also pointed out by the author in his present paper are the limitations of diagnosis. Central-nervous-system involvement usually brings with it special topographic difficulties and problems."}
{"id": "PMID:482449", "title": "[Method for studying spinal evoked potentials].", "content": "The authors describe a new method of examing evoked spinal potentials. Derivation is by means of unipolar needle electrodes which are ventrolaterally introduced into the cervical disk space as far as the posterior longitudinal ligament. Detection of a spinal potential following the stimulation of brachial and crural nerves allows to exclude the possibility of complete transverse lesion of the spinal cord or severing of the peripheral nerve or plexus.", "contents": "[Method for studying spinal evoked potentials]. The authors describe a new method of examing evoked spinal potentials. Derivation is by means of unipolar needle electrodes which are ventrolaterally introduced into the cervical disk space as far as the posterior longitudinal ligament. Detection of a spinal potential following the stimulation of brachial and crural nerves allows to exclude the possibility of complete transverse lesion of the spinal cord or severing of the peripheral nerve or plexus."}
{"id": "PMID:482450", "title": "[From paraphasia through speech disorders to disconnected thinking in schizophrenia].", "content": "The author considers paraphrasia to be the principle of the pathophysiology of the nervous system and the clinical manifestations of nervous and mental diseases, which he designates as a disintegrative deviation in the irradiation of nerve processes. By taking as a starting point some of the concepts of Wedensky, Jackson, Pavlov, and Uchtomsky, it would be possible to divide the disintegrative deviation into nondissolutive, dissolutive, and dominant deviations. The paper shows that it is possible to observe typical cases of disintegrative deviation at the speech level (with the necessary modifications) in connection with paraphasic forms of aphasia, the making of mistakes in speaking, and disconnectedness of though in schizophrenia (Kraepilin's derailments).", "contents": "[From paraphasia through speech disorders to disconnected thinking in schizophrenia]. The author considers paraphrasia to be the principle of the pathophysiology of the nervous system and the clinical manifestations of nervous and mental diseases, which he designates as a disintegrative deviation in the irradiation of nerve processes. By taking as a starting point some of the concepts of Wedensky, Jackson, Pavlov, and Uchtomsky, it would be possible to divide the disintegrative deviation into nondissolutive, dissolutive, and dominant deviations. The paper shows that it is possible to observe typical cases of disintegrative deviation at the speech level (with the necessary modifications) in connection with paraphasic forms of aphasia, the making of mistakes in speaking, and disconnectedness of though in schizophrenia (Kraepilin's derailments)."}
{"id": "PMID:482451", "title": "[Experiences and investigations with a Polish abbreviated form of the MMPI (DKO 74)].", "content": "Starting from a description of the advantages and weaknesses of the Minnesota multiphasic personality inventory (MMPI) test, the authors discuss the development, standardization, and normalization of an abbreviated form (DKO/74) which is used in Poland. Results of investigations made to determine the suitability of the method for establishing diagnoses, which included comparative examinations of various groups of Polish and G.D.R. patients (schizophrenics, neurotics, alcoholics, oncological subjects, and criminal offenders) as well as studies into factorial validity, are presented and show the test to be a suitable instrument for clinical and psychodiagnostic examinations.", "contents": "[Experiences and investigations with a Polish abbreviated form of the MMPI (DKO 74)]. Starting from a description of the advantages and weaknesses of the Minnesota multiphasic personality inventory (MMPI) test, the authors discuss the development, standardization, and normalization of an abbreviated form (DKO/74) which is used in Poland. Results of investigations made to determine the suitability of the method for establishing diagnoses, which included comparative examinations of various groups of Polish and G.D.R. patients (schizophrenics, neurotics, alcoholics, oncological subjects, and criminal offenders) as well as studies into factorial validity, are presented and show the test to be a suitable instrument for clinical and psychodiagnostic examinations."}
{"id": "PMID:482452", "title": "[Differences in the drinking behavior between alcoholic men and women in the Schwerin district].", "content": "The present paper demonstrates some typical differences in drinking behaviour between female and male alcoholics. We investigated especially the difference in quantities of consumed pure alcohol. The ratio of the mean consumption per year by women and men was 1 : 1,9. The difference in due to differences in the drinking habits and a different alcoholic tolerance in both sexes. Generally we found that in both family and society alcohol abuse is less tolerated in women.", "contents": "[Differences in the drinking behavior between alcoholic men and women in the Schwerin district]. The present paper demonstrates some typical differences in drinking behaviour between female and male alcoholics. We investigated especially the difference in quantities of consumed pure alcohol. The ratio of the mean consumption per year by women and men was 1 : 1,9. The difference in due to differences in the drinking habits and a different alcoholic tolerance in both sexes. Generally we found that in both family and society alcohol abuse is less tolerated in women."}
{"id": "PMID:482453", "title": "[Suicide attempts with drugs].", "content": "A sample of 352 subjects, who had made a suicide attempt by ingesting pills examined in the aspects of age and sex, number and origin of used tablets and combination of pill ingestion with alcohol or other methods. Younger people under 20, took nearly exclusively tablets, which they accidentally found at home. Elder patients over 50, used nearly exclusively prescribed drugs. The typical patients attitudes to this suicidal method gives us the right to speak about such suicide attempts as \"attempts by tablets\". The pharmacological substance is unimportant and plays a secondary role. The seriousness of suicide attempts depends on topical situation of person, biological and social factors; it does'nt dependent on number or pharmacological substance of tablets.", "contents": "[Suicide attempts with drugs]. A sample of 352 subjects, who had made a suicide attempt by ingesting pills examined in the aspects of age and sex, number and origin of used tablets and combination of pill ingestion with alcohol or other methods. Younger people under 20, took nearly exclusively tablets, which they accidentally found at home. Elder patients over 50, used nearly exclusively prescribed drugs. The typical patients attitudes to this suicidal method gives us the right to speak about such suicide attempts as \"attempts by tablets\". The pharmacological substance is unimportant and plays a secondary role. The seriousness of suicide attempts depends on topical situation of person, biological and social factors; it does'nt dependent on number or pharmacological substance of tablets."}
{"id": "PMID:482454", "title": "[Contents and peculiarities of the activity of psychologists in psychiatry and neurology under the conditions of a university hospital of neurological psychistry].", "content": "The qualification of clinical psychologists and their theoretically possible and practically realized employments are discussed from the practical psychologist's viewpoint and with reference to the historical development of clinical psychology.", "contents": "[Contents and peculiarities of the activity of psychologists in psychiatry and neurology under the conditions of a university hospital of neurological psychistry]. The qualification of clinical psychologists and their theoretically possible and practically realized employments are discussed from the practical psychologist's viewpoint and with reference to the historical development of clinical psychology."}
{"id": "PMID:482455", "title": "The hospital and the borderline patient: management guidelines for the community mental health center.", "content": "Controversy over the treatment of borderline patients in regression has frequently left underfined the practical approaches to hospital management of these patients. The nature and context of the regressive episode, and the implications of potential interventions for specific types of patients are basic considerations in formulating such an approach. Subgroups of borderline patients can be usefully distinguished on the basis of the major management problem to which each is particularly prone.", "contents": "The hospital and the borderline patient: management guidelines for the community mental health center. Controversy over the treatment of borderline patients in regression has frequently left underfined the practical approaches to hospital management of these patients. The nature and context of the regressive episode, and the implications of potential interventions for specific types of patients are basic considerations in formulating such an approach. Subgroups of borderline patients can be usefully distinguished on the basis of the major management problem to which each is particularly prone."}
{"id": "PMID:482456", "title": "Community mental health treatment: what works for whom?", "content": "The research literature does not establish with any consistency an integrated model of what type of patient functions best in what type of community setting. The findings do, however, identify the relationship of certain patient characteristics to re-hospitalization: number of prior hospitalizations, length of hospitalization and history of unemployment being positively related; educational level, occupational level, and race being unrelated; and sex, marital status, age, and diagnosis being inconsistently related. Also identified in the literature were those community programs that were found to be the most effective based on a review of four outcome measures including recidivism, symptomatology, social functioning, and employment.", "contents": "Community mental health treatment: what works for whom? The research literature does not establish with any consistency an integrated model of what type of patient functions best in what type of community setting. The findings do, however, identify the relationship of certain patient characteristics to re-hospitalization: number of prior hospitalizations, length of hospitalization and history of unemployment being positively related; educational level, occupational level, and race being unrelated; and sex, marital status, age, and diagnosis being inconsistently related. Also identified in the literature were those community programs that were found to be the most effective based on a review of four outcome measures including recidivism, symptomatology, social functioning, and employment."}
{"id": "PMID:482458", "title": "Day hospital versus outpatient treatment: a controlled study.", "content": "This study compares day hospitalization with traditional outpatient treatment effecting rehospitalization, symptomatology, mood, community, and vocational adjustment for 30 recently discharged schizophrenic patients. Results indicate day hospital patients were significantly more involved in work and training activities, but had no significant difference in the other areas of measurement.", "contents": "Day hospital versus outpatient treatment: a controlled study. This study compares day hospitalization with traditional outpatient treatment effecting rehospitalization, symptomatology, mood, community, and vocational adjustment for 30 recently discharged schizophrenic patients. Results indicate day hospital patients were significantly more involved in work and training activities, but had no significant difference in the other areas of measurement."}
{"id": "PMID:482459", "title": "State mental hospitals and the future.", "content": "There seems to be a general acceptance of the idea that at some future date mental hospitals will disappear to be replaced by community mental health services of various kinds. The author points out that remarkably little interest is focused on the possibility of a new model of residential care to replace the outmoded hospital. Some form of residential mental health care will probably always be needed, and should be complementary to the developing nonresidential services. A strategy for revitalizing mental hospitals is outlined.", "contents": "State mental hospitals and the future. There seems to be a general acceptance of the idea that at some future date mental hospitals will disappear to be replaced by community mental health services of various kinds. The author points out that remarkably little interest is focused on the possibility of a new model of residential care to replace the outmoded hospital. Some form of residential mental health care will probably always be needed, and should be complementary to the developing nonresidential services. A strategy for revitalizing mental hospitals is outlined."}
{"id": "PMID:482461", "title": "Clinical considerations in the prescription of group, brief, long-term, and couples psychotherapy.", "content": "Differential clinical considerations for treatment prescription as an aspect of outpatient evaluation for psychotherapy is presented in overview. The indications and contraindications presented for each of the treatment modalities of group therapy, brief therapy, long-term therapy and couples therapy are based on psychotherapy evaluation, selection criteria and outcome research to date. The information and fund of knowledge of the psychiatrist about appropriate treatment prescription matched to the patient, his problems, and his motivation to work psychotherapeutically is an essential aspect of the initial evaluation.", "contents": "Clinical considerations in the prescription of group, brief, long-term, and couples psychotherapy. Differential clinical considerations for treatment prescription as an aspect of outpatient evaluation for psychotherapy is presented in overview. The indications and contraindications presented for each of the treatment modalities of group therapy, brief therapy, long-term therapy and couples therapy are based on psychotherapy evaluation, selection criteria and outcome research to date. The information and fund of knowledge of the psychiatrist about appropriate treatment prescription matched to the patient, his problems, and his motivation to work psychotherapeutically is an essential aspect of the initial evaluation."}
{"id": "PMID:482463", "title": "On examination dreams.", "content": "The literature concerning examination dreams is reviewed, and the case of a patient who had a number of examination and examination-like dreams is described. Examination dreams are related to traumatic dreams, \"idiosyncratic\" dreams, and various behavioral expressions. In addition, constitutional factors involving impulse-defense imbalance, childhood experiences with physical difficulties and medical examinations, and ambivalent identifications and object relations seem to find representation in these dreams.", "contents": "On examination dreams. The literature concerning examination dreams is reviewed, and the case of a patient who had a number of examination and examination-like dreams is described. Examination dreams are related to traumatic dreams, \"idiosyncratic\" dreams, and various behavioral expressions. In addition, constitutional factors involving impulse-defense imbalance, childhood experiences with physical difficulties and medical examinations, and ambivalent identifications and object relations seem to find representation in these dreams."}
{"id": "PMID:482464", "title": "The search for the mother: narcissistic regression as a pathway of mourning in childhood.", "content": "Previous observers, following Freud's formulations in Mourning and Melancholia, have disagreed over the question of whether the child can mourn. To support the thesis that the child does mourn but in a different way from the adult, the author focuses on the importance of the child's identification with the lost love object, the narcissistic regression, and the associated autoerotic activities. Clinical material is presented from the analysis of a latency child and of an adult, both of whom lost their mothers during childhood.", "contents": "The search for the mother: narcissistic regression as a pathway of mourning in childhood. Previous observers, following Freud's formulations in Mourning and Melancholia, have disagreed over the question of whether the child can mourn. To support the thesis that the child does mourn but in a different way from the adult, the author focuses on the importance of the child's identification with the lost love object, the narcissistic regression, and the associated autoerotic activities. Clinical material is presented from the analysis of a latency child and of an adult, both of whom lost their mothers during childhood."}
{"id": "PMID:482466", "title": "Bulimia nervosa: an ominous variant of anorexia nervosa.", "content": "Thirty patients were selected for a prospective study according to two criteria: (i) an irresistible urge to overeat (bulimia nervosa), followed by self-induced vomiting or purging; (ii) a morbid fear of becoming fat. The majority of the patients had a previous history of true or cryptic anorexia nervosa. Self-induced vomiting and purging are secondary devices used by the patients to counteract the effects of overeating and prevent a gain in weight. These devices are dangerous for they are habit-forming and lead to potassium loss and other physical complications. In common with true anorexia nervosa, the patients were determined to keep their weight below a self-imposed threshold. Its level was set below the patient's healthy weight, defined as the weight reached before the onset of the eating disorder. In contrast with true anorexia nervosa, the patients tended to be heavier, more active sexually, and more likely to menstruate regularly and remain fertile. Depressive symptoms were often severe and distressing and led to a high risk of suicide. A theoretical model is described to emphasize the interdependence of the various symptoms and the role of self-perpetuating mechanisms in the maintenance of the disorder. The main aims of treatment are (i) to interrupt the vicious circle of overeating and self-induced vomiting (or purging), (ii) to persuade the patients to accept a higher weight. Prognosis appears less favourable than in uncomplicated anorexia nervosa.", "contents": "Bulimia nervosa: an ominous variant of anorexia nervosa. Thirty patients were selected for a prospective study according to two criteria: (i) an irresistible urge to overeat (bulimia nervosa), followed by self-induced vomiting or purging; (ii) a morbid fear of becoming fat. The majority of the patients had a previous history of true or cryptic anorexia nervosa. Self-induced vomiting and purging are secondary devices used by the patients to counteract the effects of overeating and prevent a gain in weight. These devices are dangerous for they are habit-forming and lead to potassium loss and other physical complications. In common with true anorexia nervosa, the patients were determined to keep their weight below a self-imposed threshold. Its level was set below the patient's healthy weight, defined as the weight reached before the onset of the eating disorder. In contrast with true anorexia nervosa, the patients tended to be heavier, more active sexually, and more likely to menstruate regularly and remain fertile. Depressive symptoms were often severe and distressing and led to a high risk of suicide. A theoretical model is described to emphasize the interdependence of the various symptoms and the role of self-perpetuating mechanisms in the maintenance of the disorder. The main aims of treatment are (i) to interrupt the vicious circle of overeating and self-induced vomiting (or purging), (ii) to persuade the patients to accept a higher weight. Prognosis appears less favourable than in uncomplicated anorexia nervosa."}
{"id": "PMID:482467", "title": "Plasma cortisol levels in depression and other psychiatric disorders: a study of newly admitted psychiatric patients.", "content": "Morning and evening plasma cortisol levels were checked in 123 consecutively newly admitted psychiatric patients with a variety of diagnoses. Questions asked were whether there were differences among groups with more severe illness, type of depression, alcohol abuse, or particular symptoms. Morning cortisol elevation was found in 33% of patients and was not associated with any particular diagnostic category. Evening cortisol elevation occurred in 85% of the subjects. It was significantly higher in those with unipolar depression and organic brain syndrome, also in those patients who abused alcohol regardless of diagnosis. Evening cortisol elevation was twice as common in patients with diagnoses of more severe psychiatric illness than in those with minor disorders. Further study is suggested to see if these patterns of cortisol elevation are sustained beyond the stress-of-admission period.", "contents": "Plasma cortisol levels in depression and other psychiatric disorders: a study of newly admitted psychiatric patients. Morning and evening plasma cortisol levels were checked in 123 consecutively newly admitted psychiatric patients with a variety of diagnoses. Questions asked were whether there were differences among groups with more severe illness, type of depression, alcohol abuse, or particular symptoms. Morning cortisol elevation was found in 33% of patients and was not associated with any particular diagnostic category. Evening cortisol elevation occurred in 85% of the subjects. It was significantly higher in those with unipolar depression and organic brain syndrome, also in those patients who abused alcohol regardless of diagnosis. Evening cortisol elevation was twice as common in patients with diagnoses of more severe psychiatric illness than in those with minor disorders. Further study is suggested to see if these patterns of cortisol elevation are sustained beyond the stress-of-admission period."}
{"id": "PMID:482468", "title": "Tryptophan disposition in psychiatric patients before and after stress.", "content": "Non-esterified fatty acid and total and free tryptophan were determined in the plasma of psychiatric patients unselected with respect to psychiatric diagnosis and in the plasma of normal subjects before and after physiological and psychiatric tests. Retarded patients had significantly low total and free tryptophan values which correlated negatively with agitation. Total tryptophan fell significantly after testing in the non-retarded subjects. The only biochemical abnormality significantly associated with a diagnosis of primary depression was the rise of plasma non-esterified fatty acid after testing. Thus, tryptophan abnormalities were associated more with psychiatric rating scores than with diagnoses.", "contents": "Tryptophan disposition in psychiatric patients before and after stress. Non-esterified fatty acid and total and free tryptophan were determined in the plasma of psychiatric patients unselected with respect to psychiatric diagnosis and in the plasma of normal subjects before and after physiological and psychiatric tests. Retarded patients had significantly low total and free tryptophan values which correlated negatively with agitation. Total tryptophan fell significantly after testing in the non-retarded subjects. The only biochemical abnormality significantly associated with a diagnosis of primary depression was the rise of plasma non-esterified fatty acid after testing. Thus, tryptophan abnormalities were associated more with psychiatric rating scores than with diagnoses."}
{"id": "PMID:482469", "title": "Attentional deficit in the siblings of schizophrenics.", "content": "A study of attentional deficit in the relatives of schizophrenics and in a matched control group is reported, in which the relatives are found to have poorer scores on a vigilance test and on a choice reaction time test. It is concluded that this deficit points to one of the primary symptoms of schizoprenia, and suggestions for further research are made.", "contents": "Attentional deficit in the siblings of schizophrenics. A study of attentional deficit in the relatives of schizophrenics and in a matched control group is reported, in which the relatives are found to have poorer scores on a vigilance test and on a choice reaction time test. It is concluded that this deficit points to one of the primary symptoms of schizoprenia, and suggestions for further research are made."}
{"id": "PMID:482470", "title": "Parasuicide in young Edinburgh women, 1968--75.", "content": "Between 1968 and 1975 there was a marked, non-artefactual rise in the numbers of young female parasuicides admitted to the Regional Poisoning Treatment Centre in Edinburgh. The greatest increase in rates occurred among girls aged 15--19 (for events, persons and 'first-ever' episodes). Among teenagers the most marked increase occurred for those who were married; this group also reported a greater increase than did the non-married in the frequency of physical violence and serious debt. 'Marginel' social-sexual roles have also become progressively more common in this age group. The 20- to 24-year group showed a more modest increase in rates, similar for the married and the non-married. In this age group there was no consistent pattern of change for reported violence or debt over the study period, nor were changes in these variables related to marital status. An increase in consumption of alcohol before parasuicide was noted for both age groups.", "contents": "Parasuicide in young Edinburgh women, 1968--75. Between 1968 and 1975 there was a marked, non-artefactual rise in the numbers of young female parasuicides admitted to the Regional Poisoning Treatment Centre in Edinburgh. The greatest increase in rates occurred among girls aged 15--19 (for events, persons and 'first-ever' episodes). Among teenagers the most marked increase occurred for those who were married; this group also reported a greater increase than did the non-married in the frequency of physical violence and serious debt. 'Marginel' social-sexual roles have also become progressively more common in this age group. The 20- to 24-year group showed a more modest increase in rates, similar for the married and the non-married. In this age group there was no consistent pattern of change for reported violence or debt over the study period, nor were changes in these variables related to marital status. An increase in consumption of alcohol before parasuicide was noted for both age groups."}
{"id": "PMID:482471", "title": "Influence of experience and nationality on assessment and outcome of parasuicide.", "content": "In Sheffield 544 consecutive cases of attempted suicide (parasuicide) were seen at 2 large hospitals over a 12-month period, and allocated in sequence to the junior and senior psychiatric staff, of British and foreign origin. During the follow-up period of 12 months, 31 patients had a further episode of parasuicide and 5 patients committed suicide. The outcome (as measured by repeat parasuicide or suicide) was not significantly affected by the country of origin of the psychiatrist, nor his length of training in psychiatry.", "contents": "Influence of experience and nationality on assessment and outcome of parasuicide. In Sheffield 544 consecutive cases of attempted suicide (parasuicide) were seen at 2 large hospitals over a 12-month period, and allocated in sequence to the junior and senior psychiatric staff, of British and foreign origin. During the follow-up period of 12 months, 31 patients had a further episode of parasuicide and 5 patients committed suicide. The outcome (as measured by repeat parasuicide or suicide) was not significantly affected by the country of origin of the psychiatrist, nor his length of training in psychiatry."}
{"id": "PMID:482473", "title": "Psychiatric symptoms in self-poisoning patients.", "content": "Self-poisoning patients admitted to hospital were studied for persistence of psychiatric symptoms over a 3-month period and evaluated at 3 points in time with the General Health Questionnaire and the Present State Examination. Symptoms were identified which had a high or low prevalence at 3-month follow-up. GHQ and PSE scores correlated at 0.8. The implications of the study are discussed, particularly the earlier need for out-patient help in those who had a moderate or high number of symptoms at initial interview.", "contents": "Psychiatric symptoms in self-poisoning patients. Self-poisoning patients admitted to hospital were studied for persistence of psychiatric symptoms over a 3-month period and evaluated at 3 points in time with the General Health Questionnaire and the Present State Examination. Symptoms were identified which had a high or low prevalence at 3-month follow-up. GHQ and PSE scores correlated at 0.8. The implications of the study are discussed, particularly the earlier need for out-patient help in those who had a moderate or high number of symptoms at initial interview."}
{"id": "PMID:482474", "title": "Psychiatric diagnosis in self-poisoning patients.", "content": "The Present State Examination was administered to 539 patients who attended a casualty department after an act of self-poisoning during the course of a year. Computer analysis by means of the Catego program showed that 30% of the patients had insufficient symptoms to be considered as psychiatric 'cases'. Fewer than 4% had symptoms of functional psychosis. About 60% of the patients were classed as depressed: on several indices this group was less severely ill than a group of depressives feceiving psychiatric treatment. It is suggested that psychiatric treatment is essential for only a small proportion of self-poisoning patients. The effectiveness of psychiatric treatment for the large group of patients with depressive disorders of minor severity and probably short duration has still to be evaluated.", "contents": "Psychiatric diagnosis in self-poisoning patients. The Present State Examination was administered to 539 patients who attended a casualty department after an act of self-poisoning during the course of a year. Computer analysis by means of the Catego program showed that 30% of the patients had insufficient symptoms to be considered as psychiatric 'cases'. Fewer than 4% had symptoms of functional psychosis. About 60% of the patients were classed as depressed: on several indices this group was less severely ill than a group of depressives feceiving psychiatric treatment. It is suggested that psychiatric treatment is essential for only a small proportion of self-poisoning patients. The effectiveness of psychiatric treatment for the large group of patients with depressive disorders of minor severity and probably short duration has still to be evaluated."}
{"id": "PMID:482475", "title": "The contextual threat of life events: the concept and its reliability.", "content": "The reliability of Brown's contextual measure of threat is assessed and found to be highly satisfactory. Previously inexperienced raters achieve highly reliable ratings after only brief training. The implications for the concept of the threat of life events are discussed.", "contents": "The contextual threat of life events: the concept and its reliability. The reliability of Brown's contextual measure of threat is assessed and found to be highly satisfactory. Previously inexperienced raters achieve highly reliable ratings after only brief training. The implications for the concept of the threat of life events are discussed."}
{"id": "PMID:482476", "title": "The effect of sex, marriage and age at first admission on the hospitalization of schizophrenics during 2 years following discharge.", "content": "Two hundred and eighty-two schizophrenics discharged from St John's Hospital, Stone, were followed-up. The reliability of the diagnosis was measured by the extent of agreement among the hospital clinicians. The number of readmissions and the time spent in hospital were ascertained and correlated with the sex, civil state and age at first admission of the subjects. It was found that a higher proportion of males than of females was readmitted; that the proportion of single persons among male schizophrenics is higher than among comparable age groups in the general population; that single males are more frequently admitted than single females; that the peak age of first admission for males is 10 years earlier than for females and that men whose age at first admission is below the median are more frequently readmitted than those whose age at first admission is above it. Women are more frequently married than men at the onset of schizophrenia, giving rise to the suggestion that marriage has a protective effect in schizophrenia. This hypothesis was not supported by our findings. The bearing of these findings on the course, genetics and marital handicap of schizophrenia is discussed.", "contents": "The effect of sex, marriage and age at first admission on the hospitalization of schizophrenics during 2 years following discharge. Two hundred and eighty-two schizophrenics discharged from St John's Hospital, Stone, were followed-up. The reliability of the diagnosis was measured by the extent of agreement among the hospital clinicians. The number of readmissions and the time spent in hospital were ascertained and correlated with the sex, civil state and age at first admission of the subjects. It was found that a higher proportion of males than of females was readmitted; that the proportion of single persons among male schizophrenics is higher than among comparable age groups in the general population; that single males are more frequently admitted than single females; that the peak age of first admission for males is 10 years earlier than for females and that men whose age at first admission is below the median are more frequently readmitted than those whose age at first admission is above it. Women are more frequently married than men at the onset of schizophrenia, giving rise to the suggestion that marriage has a protective effect in schizophrenia. This hypothesis was not supported by our findings. The bearing of these findings on the course, genetics and marital handicap of schizophrenia is discussed."}
{"id": "PMID:482477", "title": "An international comparison of two systems of community mental health care.", "content": "A neighbourhood health centre in the US is compared with the mental health services in an area of the UK. Patients with severe, but long-standing psychiatric illnesses, or patients with transient emotional problems are likely to be treated by a general practitioner in London and by non-medical mental health specialists in Boston. These patients receive more overall care, more specialist, non-medical care but make as many visits to a medical provider, other than a psychiatrist, in Boston as they do in London. Psychiatrists see more acutely ill patients for a shorter time than non-medical specialists in both places, and in both Boston and London there is a highly selective referral process which results in psychiatrists from both areas seeing approximately the same proportion of the population of their catchment areas. The integration of these findings with community attitudes in planning services is discussed.", "contents": "An international comparison of two systems of community mental health care. A neighbourhood health centre in the US is compared with the mental health services in an area of the UK. Patients with severe, but long-standing psychiatric illnesses, or patients with transient emotional problems are likely to be treated by a general practitioner in London and by non-medical mental health specialists in Boston. These patients receive more overall care, more specialist, non-medical care but make as many visits to a medical provider, other than a psychiatrist, in Boston as they do in London. Psychiatrists see more acutely ill patients for a shorter time than non-medical specialists in both places, and in both Boston and London there is a highly selective referral process which results in psychiatrists from both areas seeing approximately the same proportion of the population of their catchment areas. The integration of these findings with community attitudes in planning services is discussed."}
{"id": "PMID:482478", "title": "Organization and interaction in psychiatric day centres.", "content": "One of the major problems in the care of chronic patients is the design of suitable long-term environments. Following from the work of Tizard and his colleagues, a study is described which investigated the aspects of the organization, management practices and social interactions in 4 local authority day centres for the care of chronic patients in the community. It was found that these centres differed markedly in terms of their organization and management practices and that these differences were correlated with the nature and quality of staff-client (although not staff-staff) interactions. Client-oriented management attitudes were correlated with a more personal approach to clients' problems and a warmer quality of interaction. These differences did not seem attributable to different kinds of clients being involved in each centre. The results are discussed in terms of the possible causal mechanisms involved and their implications for designing systems of long-term care.", "contents": "Organization and interaction in psychiatric day centres. One of the major problems in the care of chronic patients is the design of suitable long-term environments. Following from the work of Tizard and his colleagues, a study is described which investigated the aspects of the organization, management practices and social interactions in 4 local authority day centres for the care of chronic patients in the community. It was found that these centres differed markedly in terms of their organization and management practices and that these differences were correlated with the nature and quality of staff-client (although not staff-staff) interactions. Client-oriented management attitudes were correlated with a more personal approach to clients' problems and a warmer quality of interaction. These differences did not seem attributable to different kinds of clients being involved in each centre. The results are discussed in terms of the possible causal mechanisms involved and their implications for designing systems of long-term care."}
{"id": "PMID:482479", "title": "Interactions in contingency tables: a brief discussion of alternative definitions.", "content": "The purpose of this note is to indicate how the disagreement between Tennant & Bebbington and Brown & Harris has arisen. The difference between the 2 pairs of authors is due, at least in part, to their use of different models for the analysis of the data in question. There can be no final answer as to which model is correct - the data are simply open to more than one interpretation. Nevertheless, it is interesting that the use of a multiplicative model, seemingly ignored by Brown & Harris, leads to a simple description of the data in which the 'vulnerability factor' and the 'provoking agent' may be considered to act independently on the response. Consequently, in stating that their data contain an interaction so obvious that it can be detected by 'visual inspection', Brown & Harris may have been somewhat rash.", "contents": "Interactions in contingency tables: a brief discussion of alternative definitions. The purpose of this note is to indicate how the disagreement between Tennant & Bebbington and Brown & Harris has arisen. The difference between the 2 pairs of authors is due, at least in part, to their use of different models for the analysis of the data in question. There can be no final answer as to which model is correct - the data are simply open to more than one interpretation. Nevertheless, it is interesting that the use of a multiplicative model, seemingly ignored by Brown & Harris, leads to a simple description of the data in which the 'vulnerability factor' and the 'provoking agent' may be considered to act independently on the response. Consequently, in stating that their data contain an interaction so obvious that it can be detected by 'visual inspection', Brown & Harris may have been somewhat rash."}
{"id": "PMID:482480", "title": "The extent of mental and physical ill-health of clients referred to social workers in a local authority department and a general attachment scheme.", "content": "Information regarding the physical and mental health of clients referred to social workers in a local authority intake team and to a general practice attachment scheme was collected over a 3-month period. Although returns to the Department of Health and Social Security during this period indicated that a very low proportion of clients were physically or mentally ill, it is clear that these figures greatly underestimate the extent of illness present. While social workers operating outside hospital and general practice attachments have been shown to have little contact with medical staff, in this study high proportions of their clients were ill and their social problems were often associated with their illness.", "contents": "The extent of mental and physical ill-health of clients referred to social workers in a local authority department and a general attachment scheme. Information regarding the physical and mental health of clients referred to social workers in a local authority intake team and to a general practice attachment scheme was collected over a 3-month period. Although returns to the Department of Health and Social Security during this period indicated that a very low proportion of clients were physically or mentally ill, it is clear that these figures greatly underestimate the extent of illness present. While social workers operating outside hospital and general practice attachments have been shown to have little contact with medical staff, in this study high proportions of their clients were ill and their social problems were often associated with their illness."}
{"id": "PMID:482523", "title": "Lymphocyte and granulocyte reactions during sleep deprivation.", "content": "The possible influence of 48 hr of sleep deprivation on in vitro DNA synthesis of blood lymphocytes and on the adhesiveness and intracellular, stainable activity of alkaline phosphatase in blood granulocytes was studied in twelve young male volunteers. Following the sleep deprivation, all 12 subjects showed marked reductions of DNA synthesis after stimulation with phytohemagglutinin. Pre-exposure levels were regained 5 days after terminating the vigil. No changes were noted in granulocyte adherence or alkaline phosphatase activity. The results suggest that sleep deprivation may decrease cell-mediated immune reactions and thereby impair some aspects of host defense.", "contents": "Lymphocyte and granulocyte reactions during sleep deprivation. The possible influence of 48 hr of sleep deprivation on in vitro DNA synthesis of blood lymphocytes and on the adhesiveness and intracellular, stainable activity of alkaline phosphatase in blood granulocytes was studied in twelve young male volunteers. Following the sleep deprivation, all 12 subjects showed marked reductions of DNA synthesis after stimulation with phytohemagglutinin. Pre-exposure levels were regained 5 days after terminating the vigil. No changes were noted in granulocyte adherence or alkaline phosphatase activity. The results suggest that sleep deprivation may decrease cell-mediated immune reactions and thereby impair some aspects of host defense."}
{"id": "PMID:482524", "title": "Cortical evoked potentials and extraversion.", "content": "Stimulus intensity modulation was studied in extraverts and introverts. Auditory evoked potentials were recorded in 29 subjects and visual evoked potentials in 55. In both sensory modalities, extraverts showed considerably larger amplitudes of the late components of the evoked potentials, suggesting that they were more \"open\" to stimuli than introverts. In this way, extraverts' responses to simple stimuli could be seen as similar to their interchange with the complex stimuli of the social world.", "contents": "Cortical evoked potentials and extraversion. Stimulus intensity modulation was studied in extraverts and introverts. Auditory evoked potentials were recorded in 29 subjects and visual evoked potentials in 55. In both sensory modalities, extraverts showed considerably larger amplitudes of the late components of the evoked potentials, suggesting that they were more \"open\" to stimuli than introverts. In this way, extraverts' responses to simple stimuli could be seen as similar to their interchange with the complex stimuli of the social world."}
{"id": "PMID:482525", "title": "Family attitudes reported in youth as potential predictors of cancer.", "content": "In long-term prospective study of a cohort of former medical students, men who later developed cancer reported different family attitudes in youth from those of their healthy classmates. The items checked on a Family Attitude Questionnaire by the future cancer group indicated a lack of closeness to parents compared with the items checked by the healthy group. Family attitudes of the future mental illness/suicide group resembled those of the cancer group, while those of the essential hypertension and coronary heart disease groups were like those of the healthy group. These prospective findings appear to fit with those of retrospective studies concerning early family relationships in cancer patients.", "contents": "Family attitudes reported in youth as potential predictors of cancer. In long-term prospective study of a cohort of former medical students, men who later developed cancer reported different family attitudes in youth from those of their healthy classmates. The items checked on a Family Attitude Questionnaire by the future cancer group indicated a lack of closeness to parents compared with the items checked by the healthy group. Family attitudes of the future mental illness/suicide group resembled those of the cancer group, while those of the essential hypertension and coronary heart disease groups were like those of the healthy group. These prospective findings appear to fit with those of retrospective studies concerning early family relationships in cancer patients."}
{"id": "PMID:482526", "title": "Sleep and temperature regulation during restraint stress in rats is affected by prior maternal separation.", "content": "During restraint stress, 30-day-old rats uniformly show an abrupt and persistent decrease in activity with a corresponding increase in quiet wakefulness. However, the effect of restraint on sleep and body temperature depends on whether maternal separation had previously occurred at the customary age (day 22) or 7 days earleir (day 15). If maternal separation took place on day 22, subsequent restraint on day 30 has no effect on the amount of time spend in sleep or on body temperature, relative to a comparison sgroup of unrestrained rats. But if maternal separation took place on day 15, restraint on day 30 elicits a marked initial increase in sleep and a later decrease in sleep and body temperature. The results are consistent with the interpretation that premature maternal separation retards the normal maturation of these behavioral and thermoregulatory responses to restraint stress.", "contents": "Sleep and temperature regulation during restraint stress in rats is affected by prior maternal separation. During restraint stress, 30-day-old rats uniformly show an abrupt and persistent decrease in activity with a corresponding increase in quiet wakefulness. However, the effect of restraint on sleep and body temperature depends on whether maternal separation had previously occurred at the customary age (day 22) or 7 days earleir (day 15). If maternal separation took place on day 22, subsequent restraint on day 30 has no effect on the amount of time spend in sleep or on body temperature, relative to a comparison sgroup of unrestrained rats. But if maternal separation took place on day 15, restraint on day 30 elicits a marked initial increase in sleep and a later decrease in sleep and body temperature. The results are consistent with the interpretation that premature maternal separation retards the normal maturation of these behavioral and thermoregulatory responses to restraint stress."}
{"id": "PMID:482527", "title": "Assessing personality factors in essential hypertension with a brief self-report instrument.", "content": "A 16-item self-report instrument was designed and cross-validated, comparing essential hypertensives with normotensives. After item selection using two sets of standardization groups, scores obtained from three additional sets of hypertensive and normotensive groups were significantly different. The scores were not significantly related to variables such as age, sex, socioeconomic status, hypochondriasis, social desirability or target organ involvement. The instrument's factorial structure for hypertensives consisted of factors labeled anger arousal, resentment, anxiety, and attention seeking. Hypertensives reported higher levels of hostility and anxiety than normotensives. High and low scoring subgroups on the instrument were examined on the 16 PF, with hypertensive high scorers demonstrating a different profile than low scoring hypertensives. The existence of two psychological types of essential hypertensives was suggested.", "contents": "Assessing personality factors in essential hypertension with a brief self-report instrument. A 16-item self-report instrument was designed and cross-validated, comparing essential hypertensives with normotensives. After item selection using two sets of standardization groups, scores obtained from three additional sets of hypertensive and normotensive groups were significantly different. The scores were not significantly related to variables such as age, sex, socioeconomic status, hypochondriasis, social desirability or target organ involvement. The instrument's factorial structure for hypertensives consisted of factors labeled anger arousal, resentment, anxiety, and attention seeking. Hypertensives reported higher levels of hostility and anxiety than normotensives. High and low scoring subgroups on the instrument were examined on the 16 PF, with hypertensive high scorers demonstrating a different profile than low scoring hypertensives. The existence of two psychological types of essential hypertensives was suggested."}
{"id": "PMID:482528", "title": "Emotional disturbance and cognitive deficits in hyperthyroidism.", "content": "Assessment of cognitive and emotional variables in 19 females with hyperthyroidism was made pretreatment, at 3 weeks, and after euthyroidism was established. A matched group of normal controls was similarly tested. Group differences on cognitive measures did not reach statistical significance, but cognitive deficits and symptoms of emotional disorder were significantly associated with the severity of thyroid toxicity previous to treatment. Measures of cognitive function and personality features moved towards control group values as euthyroidism was established. The implication of these findings is discussed in the context of a review of previous literature. The observed cognitive disturbance and emotional distress appear to be reflections of thyroid toxicity.", "contents": "Emotional disturbance and cognitive deficits in hyperthyroidism. Assessment of cognitive and emotional variables in 19 females with hyperthyroidism was made pretreatment, at 3 weeks, and after euthyroidism was established. A matched group of normal controls was similarly tested. Group differences on cognitive measures did not reach statistical significance, but cognitive deficits and symptoms of emotional disorder were significantly associated with the severity of thyroid toxicity previous to treatment. Measures of cognitive function and personality features moved towards control group values as euthyroidism was established. The implication of these findings is discussed in the context of a review of previous literature. The observed cognitive disturbance and emotional distress appear to be reflections of thyroid toxicity."}
{"id": "PMID:482529", "title": "Teaching the future general practitioner.", "content": "Medical students today still graduate with insufficient skills for their role as general practitioner (family doctor, primary care physician). In particular they are lacking in their ability to use psychotherapeutic and counselling techniques for patients with psychological and psychosomatic problems. The author presents the results of a study which suggest that it is unrealistic to teach skills that demand more than, say, 15 min a session on a total of four occasions. This severe limitation poses difficult questions for those teachers who customarily think in terms of 50 min a week over many months as the basis of the model for psychotherapeutic help. The challenge of these implications is considered, and some practical ways of meeting the requirements are suggested.", "contents": "Teaching the future general practitioner. Medical students today still graduate with insufficient skills for their role as general practitioner (family doctor, primary care physician). In particular they are lacking in their ability to use psychotherapeutic and counselling techniques for patients with psychological and psychosomatic problems. The author presents the results of a study which suggest that it is unrealistic to teach skills that demand more than, say, 15 min a session on a total of four occasions. This severe limitation poses difficult questions for those teachers who customarily think in terms of 50 min a week over many months as the basis of the model for psychotherapeutic help. The challenge of these implications is considered, and some practical ways of meeting the requirements are suggested."}
{"id": "PMID:482530", "title": "Psychosomatic aspects in self-help groups made up of medical patients. Presented on the example of the ostomy group.", "content": "We present the psychosomatic aspects in self-help groups containing medical patients on the examples of the ostomy group. Following the demonstration of the psychodynamic group processes, the patients' psychological characteristics are described as well as the aims of this type of self-help group. The specific ostomy group activities concern the visitors' service and the group work. Finally, we describe the psychotherapeutic interventions of the psychosomaticist.", "contents": "Psychosomatic aspects in self-help groups made up of medical patients. Presented on the example of the ostomy group. We present the psychosomatic aspects in self-help groups containing medical patients on the examples of the ostomy group. Following the demonstration of the psychodynamic group processes, the patients' psychological characteristics are described as well as the aims of this type of self-help group. The specific ostomy group activities concern the visitors' service and the group work. Finally, we describe the psychotherapeutic interventions of the psychosomaticist."}
{"id": "PMID:482531", "title": "Hebiatric psychosomatic medicine.", "content": "Adolescent disease should not be considered as something similar to infantile pathology or adult sicknesses. Special consideration must be given to the adolescent as such, taking into account the characteristics of adolescents as described in the 'Normal Adolescence Syndrome'. Symptom formation follows the same pattern as described elsewhere for children, adolescents and adults, but with very special differences in this stage of human development. Hysterical, hypochondriacal and psychotic types of psychosomatic illnesses can be described with the qualification of 'adolescent type'. Hebiatric medicine must be the specialized approach to illnesses in this developmental stage and must define its study object: the adolescent. Interviewing, clinical examination, diagnosis treatment and prognosis are of a specialized kind and the psychosomatic approach is also different. There are some more typical hebiatric pathologies that must be considered properly.", "contents": "Hebiatric psychosomatic medicine. Adolescent disease should not be considered as something similar to infantile pathology or adult sicknesses. Special consideration must be given to the adolescent as such, taking into account the characteristics of adolescents as described in the 'Normal Adolescence Syndrome'. Symptom formation follows the same pattern as described elsewhere for children, adolescents and adults, but with very special differences in this stage of human development. Hysterical, hypochondriacal and psychotic types of psychosomatic illnesses can be described with the qualification of 'adolescent type'. Hebiatric medicine must be the specialized approach to illnesses in this developmental stage and must define its study object: the adolescent. Interviewing, clinical examination, diagnosis treatment and prognosis are of a specialized kind and the psychosomatic approach is also different. There are some more typical hebiatric pathologies that must be considered properly."}
{"id": "PMID:482532", "title": "Field dependency, brain asymmetry and psychophysiological differences.", "content": "Field dependent individuals differ psychologically from field independent subjects. They use more primitive defense mechanisms, and are more responsive to social forces around them than field independent subjects. Psychophysiological differences exist between these groups which will be reviewed. Obesity and alcoholism are also related to field dependency, as well as other psychosomatic disorders. Our series of experiments show that field dependent subjects are less left cerebrally dominant, and show most elements (in subclinical form) of a left cerebral lobe (Gerstmann's) syndrome. Implications for psychosomatic medicine are noted in the psychophysiological differences seen.", "contents": "Field dependency, brain asymmetry and psychophysiological differences. Field dependent individuals differ psychologically from field independent subjects. They use more primitive defense mechanisms, and are more responsive to social forces around them than field independent subjects. Psychophysiological differences exist between these groups which will be reviewed. Obesity and alcoholism are also related to field dependency, as well as other psychosomatic disorders. Our series of experiments show that field dependent subjects are less left cerebrally dominant, and show most elements (in subclinical form) of a left cerebral lobe (Gerstmann's) syndrome. Implications for psychosomatic medicine are noted in the psychophysiological differences seen."}
{"id": "PMID:482533", "title": "Amygdaloid nucleus syndrome and dehumanization syndrome.", "content": "Through statistical tests 3 groups of impulsive-aggressive patients were studied: group 1, 100 individuals with temporal dysrhythmia; group 2, 18 individuals with temporal dysrhythmia submitted either to group analytical therapy or to psychoanalysis; group 3, 25 impulsive-aggressive individuals. Moreover the author differentiates between the amygdaloid nucleus and dehumanization syndromes, through psychoanalytical or psychiatric studies, and stresses the importance of the molding periods in relation to social factors (frustration-aggression-injustice). Amygdalotomy of the hippocampus was suggested in special cases.", "contents": "Amygdaloid nucleus syndrome and dehumanization syndrome. Through statistical tests 3 groups of impulsive-aggressive patients were studied: group 1, 100 individuals with temporal dysrhythmia; group 2, 18 individuals with temporal dysrhythmia submitted either to group analytical therapy or to psychoanalysis; group 3, 25 impulsive-aggressive individuals. Moreover the author differentiates between the amygdaloid nucleus and dehumanization syndromes, through psychoanalytical or psychiatric studies, and stresses the importance of the molding periods in relation to social factors (frustration-aggression-injustice). Amygdalotomy of the hippocampus was suggested in special cases."}
{"id": "PMID:482534", "title": "Emotional behavior and the brain.", "content": "Basic behaviors were defined as those controlled by the hypothalamus, and emotion and instinct were defined as the drives of such behaviors. A learning method showed that fear-escape is obtained from the medial hypothalamic area except for the ventromedial nucleus. It was suggested that some neural systems responsible for threat, attack, rush and retreat are overlapped in the ventromedial nucleus. Functions of the amygdala and the septum were discussed in relation to hypothalamic activity in emotional behavior. A behavioral sequence composed of stimulation, classical conditioning, drive, reward learning and behavior was discussed, and learning, anxiety in neuroses and Morita therapy were examined from the viewpoint of the behavioral sequence.", "contents": "Emotional behavior and the brain. Basic behaviors were defined as those controlled by the hypothalamus, and emotion and instinct were defined as the drives of such behaviors. A learning method showed that fear-escape is obtained from the medial hypothalamic area except for the ventromedial nucleus. It was suggested that some neural systems responsible for threat, attack, rush and retreat are overlapped in the ventromedial nucleus. Functions of the amygdala and the septum were discussed in relation to hypothalamic activity in emotional behavior. A behavioral sequence composed of stimulation, classical conditioning, drive, reward learning and behavior was discussed, and learning, anxiety in neuroses and Morita therapy were examined from the viewpoint of the behavioral sequence."}
{"id": "PMID:482535", "title": "Interaction of genetic and psychosocial factors in stress-reaction patterns: a systems approach to the investigation of stress-coping mechanisms.", "content": "Selye refers to stress as 'the nonspecific response of the body to any demand made upon it'. Our experimental data indicate that: (1) there are significant constitutional differences in the types of stress reactions exhibited by different breeds of dogs; (2) inability to achieve an adaptive consummatory response or to develop a sense of control over stressful situations may lead in susceptible individuals (low adaptation dogs) to the development of maladaptive distress reactions, evidenced by persistent psychovisceral turmoil; (3) such maladaptive distress reactions represent a physiologic substrate of anxiety and frustration; (4) exposure of the low adaptation dogs to similar stressors but under conditions where the animals can develop avoidance responses, inhibited the psychovisceral disturbances, suggesting that it is the inability to develop control over psychosocially aversive situations that is primarily responsible for psychophysiologic disorders.", "contents": "Interaction of genetic and psychosocial factors in stress-reaction patterns: a systems approach to the investigation of stress-coping mechanisms. Selye refers to stress as 'the nonspecific response of the body to any demand made upon it'. Our experimental data indicate that: (1) there are significant constitutional differences in the types of stress reactions exhibited by different breeds of dogs; (2) inability to achieve an adaptive consummatory response or to develop a sense of control over stressful situations may lead in susceptible individuals (low adaptation dogs) to the development of maladaptive distress reactions, evidenced by persistent psychovisceral turmoil; (3) such maladaptive distress reactions represent a physiologic substrate of anxiety and frustration; (4) exposure of the low adaptation dogs to similar stressors but under conditions where the animals can develop avoidance responses, inhibited the psychovisceral disturbances, suggesting that it is the inability to develop control over psychosocially aversive situations that is primarily responsible for psychophysiologic disorders."}
{"id": "PMID:482536", "title": "The psychosomatic key as a core approach to the modern plagues of mankind.", "content": "The severest plagues of manking today, from myocardial infarction to cancer, are rising exponentially the world over. For the mechanistic medical science they are mostly 'mystery diseases', as well as a mystery is their uncontrolled exponential trend. Statistics reveal that they rise higher, the more a community is developed. But why? The too easy excuse of a higher 'concrete' pollution seems unreliable, where significant difference rates are recorded by different human groups in the same environment, but exposed to different values of 'psychosomatic involvement', or delta psi. The latter symbol, meaning 'the variation of psi tone induced by a stimulus-event', belongs to a highly predictive system of quantitative psychosomatics, which enables--through a logico-mathematical tool--the ascertaining of personal levels of delta psi. Since a chronic exceeding of its tolerance threshold seems to give rise to the whole set of psychosomatic diseases, cancer perhaps included, it is suggested that the said system may help in diagnostically clearing the matter, perhaps also in controlling it.", "contents": "The psychosomatic key as a core approach to the modern plagues of mankind. The severest plagues of manking today, from myocardial infarction to cancer, are rising exponentially the world over. For the mechanistic medical science they are mostly 'mystery diseases', as well as a mystery is their uncontrolled exponential trend. Statistics reveal that they rise higher, the more a community is developed. But why? The too easy excuse of a higher 'concrete' pollution seems unreliable, where significant difference rates are recorded by different human groups in the same environment, but exposed to different values of 'psychosomatic involvement', or delta psi. The latter symbol, meaning 'the variation of psi tone induced by a stimulus-event', belongs to a highly predictive system of quantitative psychosomatics, which enables--through a logico-mathematical tool--the ascertaining of personal levels of delta psi. Since a chronic exceeding of its tolerance threshold seems to give rise to the whole set of psychosomatic diseases, cancer perhaps included, it is suggested that the said system may help in diagnostically clearing the matter, perhaps also in controlling it."}
{"id": "PMID:482537", "title": "Psychosomatic illness as a result of a deficit in ego-structure under consideration of the genetic. Dynamic, structural, and group dynamic point of view.", "content": "Psychosomatic illness is an illness of ego-structure as a result of a narcissistic deficit reassembling the phenomenology of anaclitic depression in regard to the mother and the primary group. Psychosomatic illness has to be understood on a scale of ego-illnesses related to identity defects. The psychosomatic symptom restitutes the integration of the personality and constitutes the identity of a psychosomatic patient. The role of group dynamics is particularly stressed in relation to psychogenetics, psychodynamics, and the change of symptoms.", "contents": "Psychosomatic illness as a result of a deficit in ego-structure under consideration of the genetic. Dynamic, structural, and group dynamic point of view. Psychosomatic illness is an illness of ego-structure as a result of a narcissistic deficit reassembling the phenomenology of anaclitic depression in regard to the mother and the primary group. Psychosomatic illness has to be understood on a scale of ego-illnesses related to identity defects. The psychosomatic symptom restitutes the integration of the personality and constitutes the identity of a psychosomatic patient. The role of group dynamics is particularly stressed in relation to psychogenetics, psychodynamics, and the change of symptoms."}
{"id": "PMID:482538", "title": "The right of falling ill. On pathological health behavior.", "content": "There are people who never permit themselves to fall ill. This negative illness is called by the present author: counter-hypochondriasis. Several defense mechanisms in counter-hypochondriasis are described, especially in relation to infantile roots. Pathological health is considered as a neurotic symptom formation in which disorders in object relationship are all-important.", "contents": "The right of falling ill. On pathological health behavior. There are people who never permit themselves to fall ill. This negative illness is called by the present author: counter-hypochondriasis. Several defense mechanisms in counter-hypochondriasis are described, especially in relation to infantile roots. Pathological health is considered as a neurotic symptom formation in which disorders in object relationship are all-important."}
{"id": "PMID:482539", "title": "The clinic as an interpersonal field of dynamic psychiatric treatment of psychosomatic diseases.", "content": "In G\u00fcnther Ammon's structural personality model, psychosomatic illnesses are understood to be the expression of a structural ego-defect--especially in the central ego-function of the body-ego, of identity and of constructive aggression--which owes its origin to an insufficient mother-child symbiosis. The dynamic psychiatric clinic realises an interpersonal field, into which the defects are externalised and remedied by the process of a late ego development. The clinic offers various therapeutical levels: the clinic as a whole, the analytical milieu, group and individual therapy, whereby working-through of the interdependence of patient and staff dynamics has a main integrating function.", "contents": "The clinic as an interpersonal field of dynamic psychiatric treatment of psychosomatic diseases. In G\u00fcnther Ammon's structural personality model, psychosomatic illnesses are understood to be the expression of a structural ego-defect--especially in the central ego-function of the body-ego, of identity and of constructive aggression--which owes its origin to an insufficient mother-child symbiosis. The dynamic psychiatric clinic realises an interpersonal field, into which the defects are externalised and remedied by the process of a late ego development. The clinic offers various therapeutical levels: the clinic as a whole, the analytical milieu, group and individual therapy, whereby working-through of the interdependence of patient and staff dynamics has a main integrating function."}
{"id": "PMID:482540", "title": "Psychosomatic studies of allergic disorders.", "content": "It is generally conceded that allergic disorders occur in individuals who have a hereditary or congenital allergic constitution. Clinical symptoms of allergic disorders, however, often disappear due to changes of the individuals' life situations and/or their adaptive patterns. In a comparative study of allergic predisposition in students with allergic disorder (asthmatics) and students who had become completely free from childhood asthma for more than 3 years, without specific treatment, there was no significant difference in allergic predisposition between the two groups. The same tendency was also found between adult patients with allergic disorder (asthmatics) and persons who had shown complete remission for more than 3 years, having had psychosomatic treatment. These findings suggest that allergic predisposition does not influence the prognosis of allergic disorders as much as do socio-psychological factors. It is thought that the effect of psychosomatic treatment reconditions these socio-psychological factors which disturb homeostatic balance and which facilitate the clinical manifestation based on the allergic predisposition.", "contents": "Psychosomatic studies of allergic disorders. It is generally conceded that allergic disorders occur in individuals who have a hereditary or congenital allergic constitution. Clinical symptoms of allergic disorders, however, often disappear due to changes of the individuals' life situations and/or their adaptive patterns. In a comparative study of allergic predisposition in students with allergic disorder (asthmatics) and students who had become completely free from childhood asthma for more than 3 years, without specific treatment, there was no significant difference in allergic predisposition between the two groups. The same tendency was also found between adult patients with allergic disorder (asthmatics) and persons who had shown complete remission for more than 3 years, having had psychosomatic treatment. These findings suggest that allergic predisposition does not influence the prognosis of allergic disorders as much as do socio-psychological factors. It is thought that the effect of psychosomatic treatment reconditions these socio-psychological factors which disturb homeostatic balance and which facilitate the clinical manifestation based on the allergic predisposition."}
{"id": "PMID:482541", "title": "Alexithymic features of the patients with chronic pancreatitis.", "content": "61 patients suffering from chronic pancreatitis were studied in order to clarify the psychosomatic aspects of this disease. They were classified into 5 types, out of which 3 types were examined in detail according to their personality traits and life habits. It was found that the mind-body relationship took the primary form in the psychosomatic and neurotic types, while in the organic type it took the secondary form such as distorted life habits. This finding indicated that the distortion in the personality structure and that of life habits were closely interrelated. The authors also discussed the clinical features of chronic pancreatitis from the standpoint of alexithymia. The patients with the organic type were most alexithymic, which were followed by the psychosomatic type. It was learned that alexithymic problems were reflected in the patients' life habits.", "contents": "Alexithymic features of the patients with chronic pancreatitis. 61 patients suffering from chronic pancreatitis were studied in order to clarify the psychosomatic aspects of this disease. They were classified into 5 types, out of which 3 types were examined in detail according to their personality traits and life habits. It was found that the mind-body relationship took the primary form in the psychosomatic and neurotic types, while in the organic type it took the secondary form such as distorted life habits. This finding indicated that the distortion in the personality structure and that of life habits were closely interrelated. The authors also discussed the clinical features of chronic pancreatitis from the standpoint of alexithymia. The patients with the organic type were most alexithymic, which were followed by the psychosomatic type. It was learned that alexithymic problems were reflected in the patients' life habits."}
{"id": "PMID:482542", "title": "Psoriasis: psychosomatic aspects.", "content": "On the basis of the findings of this study it could be concluded that the hereditary proneness alone is not sufficient to cause the breakout of psoriasis. Only when additional causes intervene, the disease breaks out. The importance of stress and emotional factors in causation and precipitation of the disease is discussed. The traumatic emotional experience and the aggravation of disease as result of improper approach of the society towards the patient's illness is reviewed. The impact of the disease on the treating doctor and the doctor-patient relationship is reported. Means of prevention of the breakout of the disease and the proper approach of the doctor in treating his patient is suggested and specified.", "contents": "Psoriasis: psychosomatic aspects. On the basis of the findings of this study it could be concluded that the hereditary proneness alone is not sufficient to cause the breakout of psoriasis. Only when additional causes intervene, the disease breaks out. The importance of stress and emotional factors in causation and precipitation of the disease is discussed. The traumatic emotional experience and the aggravation of disease as result of improper approach of the society towards the patient's illness is reviewed. The impact of the disease on the treating doctor and the doctor-patient relationship is reported. Means of prevention of the breakout of the disease and the proper approach of the doctor in treating his patient is suggested and specified."}
{"id": "PMID:482543", "title": "Alexithymia: anxiety and hostility in psychosomatic and psychoneurotic patients.", "content": "Verbal samples obtained from 40 psychoneurotic and 40 psychosomatic patients, matched with respect to age, sex, intelligence and some social indicators, were examined using different methods of content analysis. According to the concept of 'alexithymia' it was postulated that the restricted fantasy life and difficulty in expressing feelings attributed to psychosomatic patients would be reflected at the speech level (psycho-analytic interviews). The measurement of anxiety and hostility using the Gottschalk-Gleser scales gave the following results: with total anxiety in particular, and its subscales guilt, shame, separation and diffuse anxiety, and with hostility, the psychosomatic patients showed lower values than the neurotic patients, which were in part highly significant. The results confirm the existence of the 'psychosomatic phenomenon' but they do not permit statements concerning its etiology.", "contents": "Alexithymia: anxiety and hostility in psychosomatic and psychoneurotic patients. Verbal samples obtained from 40 psychoneurotic and 40 psychosomatic patients, matched with respect to age, sex, intelligence and some social indicators, were examined using different methods of content analysis. According to the concept of 'alexithymia' it was postulated that the restricted fantasy life and difficulty in expressing feelings attributed to psychosomatic patients would be reflected at the speech level (psycho-analytic interviews). The measurement of anxiety and hostility using the Gottschalk-Gleser scales gave the following results: with total anxiety in particular, and its subscales guilt, shame, separation and diffuse anxiety, and with hostility, the psychosomatic patients showed lower values than the neurotic patients, which were in part highly significant. The results confirm the existence of the 'psychosomatic phenomenon' but they do not permit statements concerning its etiology."}
{"id": "PMID:482544", "title": "Problems in interaction between patients undergoing long-term hemodialysis and their partners.", "content": "186 patients undergoing hemodialysis and their partners were examined with a personality inventory (Giessen test) and a questionnaire which was specially developed for this purpose. The instrument was used to measure the social interaction between patient and partner. The Giessen test was applied in four versions: self-description, description by the partner, referring to the present, referring to the past. One of the main interests of the analyses was in finding dependencies of the variables upon the types of hemodialysis setting (unit, private unit, and at home). Various correlations were found.", "contents": "Problems in interaction between patients undergoing long-term hemodialysis and their partners. 186 patients undergoing hemodialysis and their partners were examined with a personality inventory (Giessen test) and a questionnaire which was specially developed for this purpose. The instrument was used to measure the social interaction between patient and partner. The Giessen test was applied in four versions: self-description, description by the partner, referring to the present, referring to the past. One of the main interests of the analyses was in finding dependencies of the variables upon the types of hemodialysis setting (unit, private unit, and at home). Various correlations were found."}
{"id": "PMID:482545", "title": "Biofeedback and behavioral medicine: an overview.", "content": "Biofeedback is a behavioral method of achieving or enhancing voluntary control of physiological processes. Basic studies indicate that a variety of autonomic nervous system and other internal bodily changes can be modified with the method. It has been shown that specific responses and patterns of responses can be controlled. Biofeedback appears to be a promising method of altering symptoms of psychophysiological and medical disorders, e.g., high blood pressure, vascular changes in migraine, neuromuscular abnormalities, and cardiac arrhythmias. This research has stimulated a renewed interest in the role of behavior and the usefulness of behavioral principles in the etiology, treatment, and prevention of medical disorders. The term 'behavioral medicine' calls attention to this new perspective in medicine.", "contents": "Biofeedback and behavioral medicine: an overview. Biofeedback is a behavioral method of achieving or enhancing voluntary control of physiological processes. Basic studies indicate that a variety of autonomic nervous system and other internal bodily changes can be modified with the method. It has been shown that specific responses and patterns of responses can be controlled. Biofeedback appears to be a promising method of altering symptoms of psychophysiological and medical disorders, e.g., high blood pressure, vascular changes in migraine, neuromuscular abnormalities, and cardiac arrhythmias. This research has stimulated a renewed interest in the role of behavior and the usefulness of behavioral principles in the etiology, treatment, and prevention of medical disorders. The term 'behavioral medicine' calls attention to this new perspective in medicine."}
{"id": "PMID:482546", "title": "Microanalysis of the first interview with psychosomatic patients.", "content": "Microanalysis of the first interview is a technique developed by the author for the subtle translation of the verbal aspects of the doctor-patient contact. Every bit of information, derived from the patient, is translated in the traditional historical dimension, e.g., when did the complaint start, and in the here and now dimension, e.g., in how far does the information imply a cry for help and understanding. Psychosomatic patients, who are usually well adapted, suppress or repress their problems and emotions so much that they are hardly able to express these quite openly. Psychosomatic symptoms reflect the effort of the patient not to behave in a hysterical way. This means that the overt cry for help and attention, which is so characteristic for hysterical patients, is warded off. Especially the microanalytic translation of this hidden cry may be used by the interviewer to detect what the basic problems of the patient are and how the optimal climate for an efficient therapeutic contact can be established. In the Leyden Department of Psychiatry the technique is used to facilitate the training in interview technique. In the paper models will be given for the understanding of the interrelationship of psychiatric and psychosomatic symptoms.", "contents": "Microanalysis of the first interview with psychosomatic patients. Microanalysis of the first interview is a technique developed by the author for the subtle translation of the verbal aspects of the doctor-patient contact. Every bit of information, derived from the patient, is translated in the traditional historical dimension, e.g., when did the complaint start, and in the here and now dimension, e.g., in how far does the information imply a cry for help and understanding. Psychosomatic patients, who are usually well adapted, suppress or repress their problems and emotions so much that they are hardly able to express these quite openly. Psychosomatic symptoms reflect the effort of the patient not to behave in a hysterical way. This means that the overt cry for help and attention, which is so characteristic for hysterical patients, is warded off. Especially the microanalytic translation of this hidden cry may be used by the interviewer to detect what the basic problems of the patient are and how the optimal climate for an efficient therapeutic contact can be established. In the Leyden Department of Psychiatry the technique is used to facilitate the training in interview technique. In the paper models will be given for the understanding of the interrelationship of psychiatric and psychosomatic symptoms."}
{"id": "PMID:482547", "title": "New therapeutic approaches to psychosomatic patients: verbal and preverbal.", "content": "Many of the patients passed on to us are not in a position to begin with a course of treatment aiming at introspection. Here we think that inpatient psychotherapy is a valuable preparation and initiation to the psychotherapeutic process. In a therapeutic community informal contacts with other patients can provide stimuli facilitating the transposition of physical complaints or concretistically experienced life situations. Concentrative movement therapy, group therapy and other methods are helpful to the psychosomatic patient, so as to bring about an awareness of the problematical nature of own views and attitudes.", "contents": "New therapeutic approaches to psychosomatic patients: verbal and preverbal. Many of the patients passed on to us are not in a position to begin with a course of treatment aiming at introspection. Here we think that inpatient psychotherapy is a valuable preparation and initiation to the psychotherapeutic process. In a therapeutic community informal contacts with other patients can provide stimuli facilitating the transposition of physical complaints or concretistically experienced life situations. Concentrative movement therapy, group therapy and other methods are helpful to the psychosomatic patient, so as to bring about an awareness of the problematical nature of own views and attitudes."}
{"id": "PMID:482548", "title": "Psychological problems of the patient on hemodialysis and their treatment.", "content": "Patients treated by maintenance hemodialysis are subjected to many stresses. As a result, many of these patients have psychological problems. Depression is the most common psychological complication seen in them. This is reflected in a high suicide rate in these patients. Other psychological problems seen in these patients are anxiety, sexual dysfunctions, problems connected with difficulties in rehabilitation, the problem of the 'uncooperative' patients, and psychosis. Vigilant attention is required so that diagnosis may be made early and treatment started soon thereafter. Important methods of treatment are supportive psychotherapy, behavioral techniques for sexual dysfunctions, and the use of psychologically active drugs.", "contents": "Psychological problems of the patient on hemodialysis and their treatment. Patients treated by maintenance hemodialysis are subjected to many stresses. As a result, many of these patients have psychological problems. Depression is the most common psychological complication seen in them. This is reflected in a high suicide rate in these patients. Other psychological problems seen in these patients are anxiety, sexual dysfunctions, problems connected with difficulties in rehabilitation, the problem of the 'uncooperative' patients, and psychosis. Vigilant attention is required so that diagnosis may be made early and treatment started soon thereafter. Important methods of treatment are supportive psychotherapy, behavioral techniques for sexual dysfunctions, and the use of psychologically active drugs."}
{"id": "PMID:482549", "title": "Psychosomatic approach to medical rehabilitation.", "content": "The author records his views about the future of medical rehabilitation. He reports some of the evidence on which to advocate the psychosomatic approach for the management of all disease and injury prone to chronic morbidity. As the causes of distress are multiple, clinical service is required from a multiprofessional team. The success of outcome is to be judged in the social adaptation achieved, recognising that this is influenced by such intangible concepts as morale and motivation.", "contents": "Psychosomatic approach to medical rehabilitation. The author records his views about the future of medical rehabilitation. He reports some of the evidence on which to advocate the psychosomatic approach for the management of all disease and injury prone to chronic morbidity. As the causes of distress are multiple, clinical service is required from a multiprofessional team. The success of outcome is to be judged in the social adaptation achieved, recognising that this is influenced by such intangible concepts as morale and motivation."}
{"id": "PMID:482550", "title": "Hypnosis with specific relation to biofeedback and behavior therapy. Theoretical and clinical considerations.", "content": "Hypnosis as an intrapsychological and interpersonal experience is used as an integrative and amplifying procedure in relation to biofeedback mechanism and behavior therapy. The hypnotic capacity for linking cognitive to affective reactions within a feedback loop of sensory and motor imagery is presented as a dynamic approach to behavior modification during psychotherapy.", "contents": "Hypnosis with specific relation to biofeedback and behavior therapy. Theoretical and clinical considerations. Hypnosis as an intrapsychological and interpersonal experience is used as an integrative and amplifying procedure in relation to biofeedback mechanism and behavior therapy. The hypnotic capacity for linking cognitive to affective reactions within a feedback loop of sensory and motor imagery is presented as a dynamic approach to behavior modification during psychotherapy."}
{"id": "PMID:482551", "title": "Psychosomatic practice and the concept of family confrontation as therapeutical method.", "content": "Psychosocial dynamics within the family are first touched upon. Relationships between brothers and sisters play a special part in this respect, as do other factors related to personality development. It is one of the special tasks of the general practitioner to be alert to the first signs of emotional disturbances, for he is in an ideal position to intervene before more serious symptoms of illness appear. The physician has to acquire a valid picture of the family unit, with its physical and psychological strengths and short-comings. Continued flight from pressure of vital issues can lead to family neurosis in the long run. Then, the sick person is not really the carrier of disease in the traditional sense of germ or genetic consation. He is only a 'symptom' of a much more widely encompassing disease unit that has grasped a social cell like the family. Are we physicians and scientists in a position, as a consequence of our training, to see this possibility, or are such modes of thinking to be left to special fields like medical sociology? The isolated, sick person can show signs derived from a disturbed family relationship, particularly through psychosomatic disorders. Our 'psychosomatic confrontation' therapeutical method is introduced.", "contents": "Psychosomatic practice and the concept of family confrontation as therapeutical method. Psychosocial dynamics within the family are first touched upon. Relationships between brothers and sisters play a special part in this respect, as do other factors related to personality development. It is one of the special tasks of the general practitioner to be alert to the first signs of emotional disturbances, for he is in an ideal position to intervene before more serious symptoms of illness appear. The physician has to acquire a valid picture of the family unit, with its physical and psychological strengths and short-comings. Continued flight from pressure of vital issues can lead to family neurosis in the long run. Then, the sick person is not really the carrier of disease in the traditional sense of germ or genetic consation. He is only a 'symptom' of a much more widely encompassing disease unit that has grasped a social cell like the family. Are we physicians and scientists in a position, as a consequence of our training, to see this possibility, or are such modes of thinking to be left to special fields like medical sociology? The isolated, sick person can show signs derived from a disturbed family relationship, particularly through psychosomatic disorders. Our 'psychosomatic confrontation' therapeutical method is introduced."}
{"id": "PMID:482552", "title": "Fasting therapy for psychosomatic disorders in Japan.", "content": "It has been well known in Japan that fasting therapy has an excellent effect on various kinds of psychosomatic diseases. From 1967 to 1977 the therapy was carried out on about 382 cases of psychosomatic diseases in our clinic with an efficacy rate of 87%. The following diseases were considered as suitable indications for the therapy; irritable colon, behavioral disturbance of eating, functional disorders of the G-I tract, neurocirculatory asthenia, labile hypertension, variable psychosomatic symptoms of puberty, conversion hysteria and many kinds of neurosis with somatic complaints. The mechanism of effectiveness of the therapy is discussed from the psychological and biological aspects. It is concluded that the regulating mechanisms of the autonomic nervous system and endocrine system may change the whole functioning of the body including the brain, resulting in a spontaneous deconditioning of maladaptive bodily and mental behavior and an improved homeostatic adjustment to life.", "contents": "Fasting therapy for psychosomatic disorders in Japan. It has been well known in Japan that fasting therapy has an excellent effect on various kinds of psychosomatic diseases. From 1967 to 1977 the therapy was carried out on about 382 cases of psychosomatic diseases in our clinic with an efficacy rate of 87%. The following diseases were considered as suitable indications for the therapy; irritable colon, behavioral disturbance of eating, functional disorders of the G-I tract, neurocirculatory asthenia, labile hypertension, variable psychosomatic symptoms of puberty, conversion hysteria and many kinds of neurosis with somatic complaints. The mechanism of effectiveness of the therapy is discussed from the psychological and biological aspects. It is concluded that the regulating mechanisms of the autonomic nervous system and endocrine system may change the whole functioning of the body including the brain, resulting in a spontaneous deconditioning of maladaptive bodily and mental behavior and an improved homeostatic adjustment to life."}
{"id": "PMID:482554", "title": "Integration of occidental and oriental psychosomatic treatments.", "content": "The Oriental approach to life can contribute to the development of psychosomatic medicine in three major ways, revolving around its realization of the illusion of mind-body dualism, its development of practical somatopsychic techniques for learning self-control, and its orientation towards realizing our true nature. In our department we are currently exploring the use of both standard psychosomatic as well as a variety of somatopsychic techniques, with the ultimate goal of providing a meaningful integration of these approaches. While working to realize the integration of these varied approaches, we feel we are discovering some important clinical principles.", "contents": "Integration of occidental and oriental psychosomatic treatments. The Oriental approach to life can contribute to the development of psychosomatic medicine in three major ways, revolving around its realization of the illusion of mind-body dualism, its development of practical somatopsychic techniques for learning self-control, and its orientation towards realizing our true nature. In our department we are currently exploring the use of both standard psychosomatic as well as a variety of somatopsychic techniques, with the ultimate goal of providing a meaningful integration of these approaches. While working to realize the integration of these varied approaches, we feel we are discovering some important clinical principles."}
{"id": "PMID:482555", "title": "Cerebral control and attention. Science and wisdom.", "content": "Theoretical explanations of the subcortical and cortical mechanisms of attention are given. All psychophysiological approaches must be reevaluated in terms of attention. Excessive vigilance is in reality a lack of vigilance (law of optimum). Good conditioning strengthens needed vigilance and respects our liberty (liberating conditioning). The legitimacy of traditional wisdom (Zen) can be better understood by the grace of science.", "contents": "Cerebral control and attention. Science and wisdom. Theoretical explanations of the subcortical and cortical mechanisms of attention are given. All psychophysiological approaches must be reevaluated in terms of attention. Excessive vigilance is in reality a lack of vigilance (law of optimum). Good conditioning strengthens needed vigilance and respects our liberty (liberating conditioning). The legitimacy of traditional wisdom (Zen) can be better understood by the grace of science."}
{"id": "PMID:482556", "title": "Japanese psychoanalysis and the Ajase complex (Kosawa).", "content": "In 1932, Kosawa visited Freud at 19 Berggasse, and submitted to him a paper entitled: 'Two kinds of guilt feelings, the Ajase complex. In his paper, Kosawa expounded his theory of the Ajase complex, rooted in Buddhist thought. He discussed the psychological peculiarity of the Japanese as evidenced by the acceptance of oral dependency toward the mother, the repression of the resentment at the mother and 'guilt feeling over being forgiven' and a need to make restitution for harboring this evil intent. This type of guilt feeling is quite different from the guilt feeling as a fear of punishment which Freud embodied in his theory of the Oedipus complex.", "contents": "Japanese psychoanalysis and the Ajase complex (Kosawa). In 1932, Kosawa visited Freud at 19 Berggasse, and submitted to him a paper entitled: 'Two kinds of guilt feelings, the Ajase complex. In his paper, Kosawa expounded his theory of the Ajase complex, rooted in Buddhist thought. He discussed the psychological peculiarity of the Japanese as evidenced by the acceptance of oral dependency toward the mother, the repression of the resentment at the mother and 'guilt feeling over being forgiven' and a need to make restitution for harboring this evil intent. This type of guilt feeling is quite different from the guilt feeling as a fear of punishment which Freud embodied in his theory of the Oedipus complex."}
{"id": "PMID:482557", "title": "Psychosomatic medicine and cybernetics.", "content": "In our daily psychosomatic medicine clinics, we have adopted four principles from Wiener's cybernetics and von Bertalanffy's general system theory. We use the polygraphic method for the diagnosis of psychosomatic disease (black box principle). For the control of psychosomatic symptoms, we use the biofeedback method (feedback principle). We use systematic desensitization to relieve social stresses which cause psychosomatic disease (open and closed system principle). And lastly, transactional analysis, which corresponds to the information and energy principle.", "contents": "Psychosomatic medicine and cybernetics. In our daily psychosomatic medicine clinics, we have adopted four principles from Wiener's cybernetics and von Bertalanffy's general system theory. We use the polygraphic method for the diagnosis of psychosomatic disease (black box principle). For the control of psychosomatic symptoms, we use the biofeedback method (feedback principle). We use systematic desensitization to relieve social stresses which cause psychosomatic disease (open and closed system principle). And lastly, transactional analysis, which corresponds to the information and energy principle."}
{"id": "PMID:482558", "title": "Difference in the mechanism of emotion in Japanese and Westerner.", "content": "The author found that cerebral hemisphere dominance of steady-state vowels as well as human emotional and natural sounds are different between the Japanese and the Westerner; the Japanese shows a verbal hemisphere dominance, while the Westerner shows a non-verbal hemisphere dominance. He brought forward the theory of mental structure and vowels stating that such a difference in the brain related to emotional activities forms the starting point of mental structure and culture. In this study the author confirmed the above hypothesis by emotional stimulation of human olfaction.", "contents": "Difference in the mechanism of emotion in Japanese and Westerner. The author found that cerebral hemisphere dominance of steady-state vowels as well as human emotional and natural sounds are different between the Japanese and the Westerner; the Japanese shows a verbal hemisphere dominance, while the Westerner shows a non-verbal hemisphere dominance. He brought forward the theory of mental structure and vowels stating that such a difference in the brain related to emotional activities forms the starting point of mental structure and culture. In this study the author confirmed the above hypothesis by emotional stimulation of human olfaction."}
{"id": "PMID:482559", "title": "Yoga as therapy in psychosomatic medicine.", "content": "Yoga as therapy with psychosomatic disorders has been practiced for many centuries in India, and only recently has become utilized for this purpose in other countries. The yoga system evolved as a 'system of liberation' to allow man to discriminate between his ego-self and pure consciousness, and as such, its medical benefits are really 'side-effects'. Integral yoga practice, however, with which many other self-regulatory somatopsychic approaches have much in common, consists of a holistic technology which functions to restore optimal homeostatis by a variety of special techniques not found in other approaches. Clinical observations of psychosomatic patients indicate that their distorted somatopsychic functioning necessitates their practice of yoga-like therapy. A review of the clinical evidence available indicates that yoga practice has proven most effective with a wide range of psychosomatic and psychiatric disorders. The effectiveness of yoga can be partially understood in terms of neurophysiological theory.", "contents": "Yoga as therapy in psychosomatic medicine. Yoga as therapy with psychosomatic disorders has been practiced for many centuries in India, and only recently has become utilized for this purpose in other countries. The yoga system evolved as a 'system of liberation' to allow man to discriminate between his ego-self and pure consciousness, and as such, its medical benefits are really 'side-effects'. Integral yoga practice, however, with which many other self-regulatory somatopsychic approaches have much in common, consists of a holistic technology which functions to restore optimal homeostatis by a variety of special techniques not found in other approaches. Clinical observations of psychosomatic patients indicate that their distorted somatopsychic functioning necessitates their practice of yoga-like therapy. A review of the clinical evidence available indicates that yoga practice has proven most effective with a wide range of psychosomatic and psychiatric disorders. The effectiveness of yoga can be partially understood in terms of neurophysiological theory."}
{"id": "PMID:482560", "title": "The psychosomatic theory of bronchial asthma.", "content": "The author discusses the development of the psychosomatic asthma theory as a paradigm of theory formation in psychomatic medicine. The first formulation of the theory was based on clinical and psychiatric observations. It was tested by psychological, physiological and experimental methods and as a result was reformulated and extended. In its present form it regards asthmatic breathing as a reaction of a predisposed personality structure (partly hereditary, partly acquired during a youth situation in which overprotection by a domineering parent played a large role), to an ambivalent conflict with a key figure. The resulting frustration is not acted out by aggressive, flight, or depressive behaviour, but inhibited; thereby the motoric and verbal discharges are displaced into (substituted by) a respiratory behaviour pattern, which is characterised by an abnormally forceful contraction of the abdominal muscles during the expiration. The resulting high intraabdominal pressure is transmitted into the thorax where it pushes the posterior membranaceous wall of the trachea and large bronchi forward into the lumen and thus produces a long stretched obstruction of the large airways. The passage of the air through the compressed large air passages under high pressure and low velocity is the mechanism which causes the typical wheeze and the other manifestations of the asthmatic airway obstruction. A hypothesis is suggested for the ways in which this psychoneurogenic respiratory behaviour contributes to the so-called bronchial hyper-reactivity and the secondary development of allergies.", "contents": "The psychosomatic theory of bronchial asthma. The author discusses the development of the psychosomatic asthma theory as a paradigm of theory formation in psychomatic medicine. The first formulation of the theory was based on clinical and psychiatric observations. It was tested by psychological, physiological and experimental methods and as a result was reformulated and extended. In its present form it regards asthmatic breathing as a reaction of a predisposed personality structure (partly hereditary, partly acquired during a youth situation in which overprotection by a domineering parent played a large role), to an ambivalent conflict with a key figure. The resulting frustration is not acted out by aggressive, flight, or depressive behaviour, but inhibited; thereby the motoric and verbal discharges are displaced into (substituted by) a respiratory behaviour pattern, which is characterised by an abnormally forceful contraction of the abdominal muscles during the expiration. The resulting high intraabdominal pressure is transmitted into the thorax where it pushes the posterior membranaceous wall of the trachea and large bronchi forward into the lumen and thus produces a long stretched obstruction of the large airways. The passage of the air through the compressed large air passages under high pressure and low velocity is the mechanism which causes the typical wheeze and the other manifestations of the asthmatic airway obstruction. A hypothesis is suggested for the ways in which this psychoneurogenic respiratory behaviour contributes to the so-called bronchial hyper-reactivity and the secondary development of allergies."}
{"id": "PMID:482561", "title": "Definition, practice, and teaching of psychosomatics. A global survey.", "content": "Two sets of overlapping questionnaires were distributed world-wide via college members to 200 psychiatrists and 200 physicians. 50% of psychiatrists (n = 101) and 30% of physicians (n = 63), replied, representing 25 countries. Areas surveyed include definitions of 'psychosomatics', physician-psychiatrist attitudes, liaison activities, teaching patterns and methods, and professional affiliation of respondents. Comparative statistical analysis of the two groups reveals significant response differences in certain conceptualizations of 'psychosomatics' and in the diagnostic distribution of referred patients. Cross-continental comparisons revealed greater acceptance of the term 'psychosomatics' by respondents from Asia, differences between North American and European conceptualizations, and a stronger attachment of the field in the Far East to academic departments of medicine as compared with the West.", "contents": "Definition, practice, and teaching of psychosomatics. A global survey. Two sets of overlapping questionnaires were distributed world-wide via college members to 200 psychiatrists and 200 physicians. 50% of psychiatrists (n = 101) and 30% of physicians (n = 63), replied, representing 25 countries. Areas surveyed include definitions of 'psychosomatics', physician-psychiatrist attitudes, liaison activities, teaching patterns and methods, and professional affiliation of respondents. Comparative statistical analysis of the two groups reveals significant response differences in certain conceptualizations of 'psychosomatics' and in the diagnostic distribution of referred patients. Cross-continental comparisons revealed greater acceptance of the term 'psychosomatics' by respondents from Asia, differences between North American and European conceptualizations, and a stronger attachment of the field in the Far East to academic departments of medicine as compared with the West."}
{"id": "PMID:482562", "title": "Goals of education in psychosomatic medicine.", "content": "The modern concept of psychosomatic medicine is based on a multifactorial, interactional systems model for all diseases in which the behavioral and social dimensions play important roles in the pathogenesis, manifestation, course, and outcome. This psychosomatic approach forms the philosophical underpinning of a modern educational program. A general goal of education for all physicians is understanding the behavioral and psychosocial factors in all illness. Specific goals for the primary physician and the psychiatrist are the instillation of a holistic attitude in patient care through systematic training, and the attainment of skills and knowledge to integrate behavioral and biomedical sciences, respectively. This includes specific diagnostic, treatment, and educational skills. Yale's multilevel educational program in psychosomatic medicine is described.", "contents": "Goals of education in psychosomatic medicine. The modern concept of psychosomatic medicine is based on a multifactorial, interactional systems model for all diseases in which the behavioral and social dimensions play important roles in the pathogenesis, manifestation, course, and outcome. This psychosomatic approach forms the philosophical underpinning of a modern educational program. A general goal of education for all physicians is understanding the behavioral and psychosocial factors in all illness. Specific goals for the primary physician and the psychiatrist are the instillation of a holistic attitude in patient care through systematic training, and the attainment of skills and knowledge to integrate behavioral and biomedical sciences, respectively. This includes specific diagnostic, treatment, and educational skills. Yale's multilevel educational program in psychosomatic medicine is described."}
{"id": "PMID:482563", "title": "Teaching psychosomatic approaches as a basis of clinical medicine.", "content": "We have introduced our idea of psychosomatic approach as a basis of clinical medicine and the present state of psychosomatic teaching and training in our department as well as in other university hospitals in Japan. In teaching and training medical students and postgraduates, we aim at producing psychosomatically minded clinical physicians. For this purpose, multidisciplinary and teamwork approaches in cooperation with paramedicals are needed.", "contents": "Teaching psychosomatic approaches as a basis of clinical medicine. We have introduced our idea of psychosomatic approach as a basis of clinical medicine and the present state of psychosomatic teaching and training in our department as well as in other university hospitals in Japan. In teaching and training medical students and postgraduates, we aim at producing psychosomatically minded clinical physicians. For this purpose, multidisciplinary and teamwork approaches in cooperation with paramedicals are needed."}
{"id": "PMID:482564", "title": "German developments in teaching psychosomatics and psychotherapy to medical students.", "content": "The necessities for a reform of teaching German medical students were manifold. With the help of those doctors responsible for primary care and by founding a society representing all behavioural scientists within medical teaching it was possible to firmly institutionalize psychosomatics and psychotherapy within the new Medical Approbation Law. It is too early to assess the impact of this drastic reform but some of its advantages are already obvious.", "contents": "German developments in teaching psychosomatics and psychotherapy to medical students. The necessities for a reform of teaching German medical students were manifold. With the help of those doctors responsible for primary care and by founding a society representing all behavioural scientists within medical teaching it was possible to firmly institutionalize psychosomatics and psychotherapy within the new Medical Approbation Law. It is too early to assess the impact of this drastic reform but some of its advantages are already obvious."}
{"id": "PMID:482565", "title": "Does history-taking affect learning of attitudes?", "content": "How can medical students adopt a patient-oriented approach? During the past 7 years approximately 60 preclinical and clinical students joined dynamically oriented group sessions on medical history-taking. An open-ended question technique was used. The student's expectations of the patient were monitored by the repertory grid technique using a self-developed dyad grid form which allowed the estimation of reciprocal expectations as seen by the student. A main component analysis of the ratings obtained was performed. The development in one group was taken as representative of the general development in the different groups. Two main components (MC I, II) were found to account for approximately 40 and 25% of the total variance (distance/empathy and dominance/dependence). The student's main concern is centered on the problem of dependence, MC II. He expects the patient to cling to him. Similarly he feels dependent on the psychosomatic lecturer. He feels as though he is the lecturer's patient. During group work the patient is seen to be less clinging. The student learns that he is not his lecturer's patient but his junior partner. He changes his professional ego-ideal as a future doctor. This is modelled according to his own relationship with the lecturer. It is not modelled according to the usual doctor-patient relationship as perceived by the student. The main characteristic of the change is a lessening of dependency needs as felt in the relationship to the patient. The findings are interpreted as an attitudinal change in favour of a more patient-oriented attitude. The change was affected through confrontation with the patient's needs. Conclusions were drawn: The student has to be trained how to cope with dependency needs. Special learning settings have to be provided which are staffed with patient-oriented physicians.", "contents": "Does history-taking affect learning of attitudes? How can medical students adopt a patient-oriented approach? During the past 7 years approximately 60 preclinical and clinical students joined dynamically oriented group sessions on medical history-taking. An open-ended question technique was used. The student's expectations of the patient were monitored by the repertory grid technique using a self-developed dyad grid form which allowed the estimation of reciprocal expectations as seen by the student. A main component analysis of the ratings obtained was performed. The development in one group was taken as representative of the general development in the different groups. Two main components (MC I, II) were found to account for approximately 40 and 25% of the total variance (distance/empathy and dominance/dependence). The student's main concern is centered on the problem of dependence, MC II. He expects the patient to cling to him. Similarly he feels dependent on the psychosomatic lecturer. He feels as though he is the lecturer's patient. During group work the patient is seen to be less clinging. The student learns that he is not his lecturer's patient but his junior partner. He changes his professional ego-ideal as a future doctor. This is modelled according to his own relationship with the lecturer. It is not modelled according to the usual doctor-patient relationship as perceived by the student. The main characteristic of the change is a lessening of dependency needs as felt in the relationship to the patient. The findings are interpreted as an attitudinal change in favour of a more patient-oriented attitude. The change was affected through confrontation with the patient's needs. Conclusions were drawn: The student has to be trained how to cope with dependency needs. Special learning settings have to be provided which are staffed with patient-oriented physicians."}
{"id": "PMID:482566", "title": "A reappraisal of some psychosomatic concepts.", "content": "The paper presents a critical reappraisal of some psychosomatic concepts which have become entrenched in the literature. The limitations of concepts of specificity relating to stimuli, emotional changes and various psychosocial stresses are discussed. The available evidence indicates the importance of the patient's personality, but does not support the claims of specific personality types for each disorder. Concepts of aetiology are discussed. The importance of heterogeneity rather than homogeneity is stressed. Relationships between psychiatric and psychosomatic disorders are considered. The significance of somatopsychic as well as psychosomatic sequences of events are emphasised. Finally, the importance of multifactorial causation in psychosomatic disorders is underlined.", "contents": "A reappraisal of some psychosomatic concepts. The paper presents a critical reappraisal of some psychosomatic concepts which have become entrenched in the literature. The limitations of concepts of specificity relating to stimuli, emotional changes and various psychosocial stresses are discussed. The available evidence indicates the importance of the patient's personality, but does not support the claims of specific personality types for each disorder. Concepts of aetiology are discussed. The importance of heterogeneity rather than homogeneity is stressed. Relationships between psychiatric and psychosomatic disorders are considered. The significance of somatopsychic as well as psychosomatic sequences of events are emphasised. Finally, the importance of multifactorial causation in psychosomatic disorders is underlined."}
{"id": "PMID:482567", "title": "On education and research in ecological medicine (Psychosomatic medicine). Psychosomatic medicine of the year 2000.", "content": "Because advances in our field have increased the complexity of our work, I feel that the name of this discipline should be changed. I suggest a more pragmatic term, namely, Ecological Medicine. Ecological Medicine is a science relating to physiological, biological, genetic, socioeconomic, cultural, etc., changes now taking place in our cosmos. It is concerned with disease and health processes of several components of the living organism. Its task is to recognize, evaluate, prevent, and treat all the above variables which are in integral part of the disease process. I should like to stress here that the central thinking must be a strategic one: to devise and apply means, ways, and methods for the exchange of information between those working in clinical and humanistic disciplines and of those in laboratories. In other words, I, personally, believe that the research scientists, who are working in any laboratory, cannot afford to ignore the psychological, socioeconomic and cultural factors affecting people's health. Consequently, they must, on one hand, orient themselves in accordance with the above-mentioned demands, and collaborate with the scientists of the humanistic disciplines, on the other. Thus, in this way, only the anthropos can be thoroughly and completely investigated as a unique psychophysiological, socioeconomic, and cultural ecologic entity.", "contents": "On education and research in ecological medicine (Psychosomatic medicine). Psychosomatic medicine of the year 2000. Because advances in our field have increased the complexity of our work, I feel that the name of this discipline should be changed. I suggest a more pragmatic term, namely, Ecological Medicine. Ecological Medicine is a science relating to physiological, biological, genetic, socioeconomic, cultural, etc., changes now taking place in our cosmos. It is concerned with disease and health processes of several components of the living organism. Its task is to recognize, evaluate, prevent, and treat all the above variables which are in integral part of the disease process. I should like to stress here that the central thinking must be a strategic one: to devise and apply means, ways, and methods for the exchange of information between those working in clinical and humanistic disciplines and of those in laboratories. In other words, I, personally, believe that the research scientists, who are working in any laboratory, cannot afford to ignore the psychological, socioeconomic and cultural factors affecting people's health. Consequently, they must, on one hand, orient themselves in accordance with the above-mentioned demands, and collaborate with the scientists of the humanistic disciplines, on the other. Thus, in this way, only the anthropos can be thoroughly and completely investigated as a unique psychophysiological, socioeconomic, and cultural ecologic entity."}
{"id": "PMID:482568", "title": "Liaison psychiatry in the West: the view from 1977.", "content": "Current status of consultation-liaison psychiatry in the West is reviewed. Its aims, functions, and scope in diagnosis and treatment of general hospital patients outside of psychiatric departments are described. A critical appraisal is made with regards to the triadic relationship of its participants. Its benefits vis-\u00e0-vis resistances are illuminated in the context of psychosocial process. Its role in research and medical education is recounted. Thoughts about its future developments are offered.", "contents": "Liaison psychiatry in the West: the view from 1977. Current status of consultation-liaison psychiatry in the West is reviewed. Its aims, functions, and scope in diagnosis and treatment of general hospital patients outside of psychiatric departments are described. A critical appraisal is made with regards to the triadic relationship of its participants. Its benefits vis-\u00e0-vis resistances are illuminated in the context of psychosocial process. Its role in research and medical education is recounted. Thoughts about its future developments are offered."}
{"id": "PMID:482574", "title": "The Job Corps experience with health problems among disadvantaged youth.", "content": "Health problems in a disadvantaged group of young people were studied by analysis of Job Corps screening and medical termination data obtained during fiscal year 1975. The Job Corps is a federally funded, residential vocational training program. During fiscal year 1975, the program was conducted in 60 centers throughout the United States. Corpsmembers (ages 16-21) come from poverty backgrounds; in 1975, 55 percent were black. Approximately 1 percent of applicants with serious health problems are screened out by a nonprofessional procedure. Comprehensive health services are provided to corpsmembers; however, limited funds and interference with training prevent continuing care for pregnancies and some serious illness and injuries. Corpsmembers with these conditions are terminated from the Job Corps, and arrangements are made for care in their communities. Less than 2 percent of the corpsmembers were terminated for medical reasons during fiscal year 1975. Pregnancy accounted for 359 terminations. Of 44,390 corpsmembers, 457 were terminated for illness or injury. The majority of these terminations were for mental health problems; trauma was the next largest category. During fiscal year 1975, 22 deaths occurred; 21 were from accidents or suicide. The low medical termination rate among these socioeconomically disadvantaged young people suggests that maintenance of relatively good health is possible over a short period through provision of basic health care and health education, which can be provided largely by allied health personnel under professional supervision.", "contents": "The Job Corps experience with health problems among disadvantaged youth. Health problems in a disadvantaged group of young people were studied by analysis of Job Corps screening and medical termination data obtained during fiscal year 1975. The Job Corps is a federally funded, residential vocational training program. During fiscal year 1975, the program was conducted in 60 centers throughout the United States. Corpsmembers (ages 16-21) come from poverty backgrounds; in 1975, 55 percent were black. Approximately 1 percent of applicants with serious health problems are screened out by a nonprofessional procedure. Comprehensive health services are provided to corpsmembers; however, limited funds and interference with training prevent continuing care for pregnancies and some serious illness and injuries. Corpsmembers with these conditions are terminated from the Job Corps, and arrangements are made for care in their communities. Less than 2 percent of the corpsmembers were terminated for medical reasons during fiscal year 1975. Pregnancy accounted for 359 terminations. Of 44,390 corpsmembers, 457 were terminated for illness or injury. The majority of these terminations were for mental health problems; trauma was the next largest category. During fiscal year 1975, 22 deaths occurred; 21 were from accidents or suicide. The low medical termination rate among these socioeconomically disadvantaged young people suggests that maintenance of relatively good health is possible over a short period through provision of basic health care and health education, which can be provided largely by allied health personnel under professional supervision."}
{"id": "PMID:482575", "title": "Evidence from the National Survey of Family Growth. Work during pregnancy and subsequent hospitalization of mothers and infants.", "content": "Large and increasing proportions of women work late into pregnancy and resume work soon after delivery. If work in those periods injures their health or that of their infants, this trend would be of public health concern. Data on ever-married primaparas from the National Survey of Family Growth conducted by the National Center for Health Statistics were used to investigate the relationship between working in the last trimester of pregnancy and two indicators of illness--hospitalization of women for complications of pregnancy and hospitalization of their infants during the first year of life. Hospitalization of the mother or child occurred for 15.0 percent of the primaparas. For primaparas who worked in the last trimester of pregnancy, the percentage was slightly higher--17.1 percent. In two groups, black women and women without hospital insurance for delivery, the percentage of mothers or infants hospitalized was much higher among the mothers who worked in the third trimester than among those who did not. The association of working late in pregnancy with higher rates of hospitalization does not mean, necessarily, that working is a cause of hospitalization. It does indicate, however, the need for epidemiologic and medical research on the relationship.", "contents": "Evidence from the National Survey of Family Growth. Work during pregnancy and subsequent hospitalization of mothers and infants. Large and increasing proportions of women work late into pregnancy and resume work soon after delivery. If work in those periods injures their health or that of their infants, this trend would be of public health concern. Data on ever-married primaparas from the National Survey of Family Growth conducted by the National Center for Health Statistics were used to investigate the relationship between working in the last trimester of pregnancy and two indicators of illness--hospitalization of women for complications of pregnancy and hospitalization of their infants during the first year of life. Hospitalization of the mother or child occurred for 15.0 percent of the primaparas. For primaparas who worked in the last trimester of pregnancy, the percentage was slightly higher--17.1 percent. In two groups, black women and women without hospital insurance for delivery, the percentage of mothers or infants hospitalized was much higher among the mothers who worked in the third trimester than among those who did not. The association of working late in pregnancy with higher rates of hospitalization does not mean, necessarily, that working is a cause of hospitalization. It does indicate, however, the need for epidemiologic and medical research on the relationship."}
{"id": "PMID:482578", "title": "Completeness and accuracy of reporting induced abortions performed in Hawaii's hospitals, 1970--74.", "content": "This study examined the completeness and accuracy of reporting induced abortions to the official agency by following up documented cases of abortions performed in hospitals in Hawaii for the years 1970--74. The following two sets of records were initially linked by computer on the basis of first name, maiden name, date of the event, age, and ethnic background: (a) a total of 18,531 induced abortions ascertained in hospitals with complete linking information and (b) official fetal death and live birth records filed at the State health department. Overall, 96.1 percent of the hospital abortions were matched in the official certificates. Of 17,550 abortions classified as elective, 98.7 percent were so recorded in the certificate file, whereas the remainder were reported as mostly either therapeutic or unknown for cause of fetal death. When the probability of matches for those abortions without complete linking information and possible errors in the information were considered, the estimates of underreporting to the health department were a maximum of 3.9 percent and a minimum of 1.2 percent; the mean value was 2.6 percent. The extent of underreporting of induced abortions is becoming serious with the widespread practice of abortion procedures in nonhospital institutions in recent years. The results of the study also demonstrated the utility of the record-linkage process in checking data for completeness and accuracy or studying separate vital events on the same persons.", "contents": "Completeness and accuracy of reporting induced abortions performed in Hawaii's hospitals, 1970--74. This study examined the completeness and accuracy of reporting induced abortions to the official agency by following up documented cases of abortions performed in hospitals in Hawaii for the years 1970--74. The following two sets of records were initially linked by computer on the basis of first name, maiden name, date of the event, age, and ethnic background: (a) a total of 18,531 induced abortions ascertained in hospitals with complete linking information and (b) official fetal death and live birth records filed at the State health department. Overall, 96.1 percent of the hospital abortions were matched in the official certificates. Of 17,550 abortions classified as elective, 98.7 percent were so recorded in the certificate file, whereas the remainder were reported as mostly either therapeutic or unknown for cause of fetal death. When the probability of matches for those abortions without complete linking information and possible errors in the information were considered, the estimates of underreporting to the health department were a maximum of 3.9 percent and a minimum of 1.2 percent; the mean value was 2.6 percent. The extent of underreporting of induced abortions is becoming serious with the widespread practice of abortion procedures in nonhospital institutions in recent years. The results of the study also demonstrated the utility of the record-linkage process in checking data for completeness and accuracy or studying separate vital events on the same persons."}
{"id": "PMID:482580", "title": "Hyperpyrexia due to air-conditioning failure in a nursing home.", "content": "During the period August 9--13, 1976, 21 of 89 residents of a nursing home in southeastern Florida had sudden onset of fever--temperature greater than 38.1 degrees C (100.6 degrees F) with no accompanying symptoms. Five residents, whose temperatures ranged from 39.4 degrees C (103 degrees F) to 41.3 degrees C (106.4 degrees F), died. No viral or bacterial pathogens were isolated. None of the nursing home's 123 employees and volunteers had similar illness. The air-conditioning system was shut down for repairs from August 9 through August 12, a time when recorded peak temperatures outdoors ranged from 30.6 degrees C (87 degrees F) to 32.2 degrees C (90 degrees F). The only recorded temperature inside the nursing home for that period was 31.7 degrees C (89 degrees F). Laboratory and epidemiologic data were consistent with the theory that the illness was due to hyperpyrexia, secondary to environmental conditions, rather than to an infectious agent. The episode was considered analogous to the nonspecific increased mortality of elderly and chronically ill persons during an urban heat wave. The episode points up the need for prompt recognition and intervention when there is the potential for heat stress in elderly and chronically ill patients. Buildings housing such patients must be designed so that alternative ventilation can be provided when the central air-conditioning system fails.", "contents": "Hyperpyrexia due to air-conditioning failure in a nursing home. During the period August 9--13, 1976, 21 of 89 residents of a nursing home in southeastern Florida had sudden onset of fever--temperature greater than 38.1 degrees C (100.6 degrees F) with no accompanying symptoms. Five residents, whose temperatures ranged from 39.4 degrees C (103 degrees F) to 41.3 degrees C (106.4 degrees F), died. No viral or bacterial pathogens were isolated. None of the nursing home's 123 employees and volunteers had similar illness. The air-conditioning system was shut down for repairs from August 9 through August 12, a time when recorded peak temperatures outdoors ranged from 30.6 degrees C (87 degrees F) to 32.2 degrees C (90 degrees F). The only recorded temperature inside the nursing home for that period was 31.7 degrees C (89 degrees F). Laboratory and epidemiologic data were consistent with the theory that the illness was due to hyperpyrexia, secondary to environmental conditions, rather than to an infectious agent. The episode was considered analogous to the nonspecific increased mortality of elderly and chronically ill persons during an urban heat wave. The episode points up the need for prompt recognition and intervention when there is the potential for heat stress in elderly and chronically ill patients. Buildings housing such patients must be designed so that alternative ventilation can be provided when the central air-conditioning system fails."}
{"id": "PMID:482579", "title": "Management issues in the organization and delivery of family planning services.", "content": "Statewide family planning programs have been developed primarily in the Southeast and in a few other States of the nation. They are managed by State public health agencies with a few exceptions. This paper presents issues which are of importance to persons and agencies interested in developing a statewide family planning program; namely State support, allocation of funds, setting goals based on impact rather than efficiency of services, secondary sources of funding, and patient data systems. Arguments for a statewide program include the maximum use of available resources (for example, title V maternal and child health funds), the opportunity to distribute resources equitably throughout the State, the development of a statewide third-party reimbursement system, the opportunity to develop evaluation mechanisms, support for starting a system of fee collection, and the use of a statewide patient data system. Arguments against a State program include some loss of local control of a project, possible organizational battles within State agencies, State political domination of program policy, and a possible shift of funding away from existing strong projects. In the early 1970s, development of statewide systems was coupled with a rapid increase of funding when broad coverage of services and accessibility were key factors. At the present, categorical funding is no longer increasing, and efficiency and maximization of resources are becoming more important.", "contents": "Management issues in the organization and delivery of family planning services. Statewide family planning programs have been developed primarily in the Southeast and in a few other States of the nation. They are managed by State public health agencies with a few exceptions. This paper presents issues which are of importance to persons and agencies interested in developing a statewide family planning program; namely State support, allocation of funds, setting goals based on impact rather than efficiency of services, secondary sources of funding, and patient data systems. Arguments for a statewide program include the maximum use of available resources (for example, title V maternal and child health funds), the opportunity to distribute resources equitably throughout the State, the development of a statewide third-party reimbursement system, the opportunity to develop evaluation mechanisms, support for starting a system of fee collection, and the use of a statewide patient data system. Arguments against a State program include some loss of local control of a project, possible organizational battles within State agencies, State political domination of program policy, and a possible shift of funding away from existing strong projects. In the early 1970s, development of statewide systems was coupled with a rapid increase of funding when broad coverage of services and accessibility were key factors. At the present, categorical funding is no longer increasing, and efficiency and maximization of resources are becoming more important."}
{"id": "PMID:482585", "title": "Systemic lupus with nephritis: a long-term study.", "content": "Seventy-one patients with systemic lupus erythematosus and clinical evidence of nephritis were seen during a 15-year period, and followed for a mean of seven years. Survival was calculated to be 76 per cent at five years and 57 per cent at ten years from onset of clinical nephritis; and 80, 65, 55 and 55 per cent five, ten, fifteen and twenty years from onset of clinical lupus. Renal biopsies showed mild or focal lesions in 30 per cent of patients, membranous lesions in 14 per cent and diffuse proliferative lesions in 55 per cent. However, there was no difference in the long-term outcome of the different histological groups. Nineteen patients (27 per cent) died during follow up, eleven from renal failure, six from sepsis and two from cerebral lupus. Death in renal failure is now usually a late event in lupus, even in patients with clinical evidence of severe nephritis. The prognosis of even severe lupus nephritis is now better than formerly reported. Reducing the dose of corticosteroid drugs, by the use of cytotoxic drugs such as azathioprine may have diminished the mortality from cardiovascular complications. Side effects of treatment, however, remain an important cause of death and morbidity.", "contents": "Systemic lupus with nephritis: a long-term study. Seventy-one patients with systemic lupus erythematosus and clinical evidence of nephritis were seen during a 15-year period, and followed for a mean of seven years. Survival was calculated to be 76 per cent at five years and 57 per cent at ten years from onset of clinical nephritis; and 80, 65, 55 and 55 per cent five, ten, fifteen and twenty years from onset of clinical lupus. Renal biopsies showed mild or focal lesions in 30 per cent of patients, membranous lesions in 14 per cent and diffuse proliferative lesions in 55 per cent. However, there was no difference in the long-term outcome of the different histological groups. Nineteen patients (27 per cent) died during follow up, eleven from renal failure, six from sepsis and two from cerebral lupus. Death in renal failure is now usually a late event in lupus, even in patients with clinical evidence of severe nephritis. The prognosis of even severe lupus nephritis is now better than formerly reported. Reducing the dose of corticosteroid drugs, by the use of cytotoxic drugs such as azathioprine may have diminished the mortality from cardiovascular complications. Side effects of treatment, however, remain an important cause of death and morbidity."}
{"id": "PMID:482581", "title": "Genetic considerations in human cancer incidence.", "content": "Analysis by the methods of genetic demography can offer plausible explanations for the unusual distribution of cancer in an area of high incidence. The important demographic characteristics include inbreeding, founder effect, and racial admixture. Inbreeding would elevate cancer incidence if autosomal recessive genes played a role in cancer etiology. Founder effect would limit this phenomenon to those recessive genes observed in the founding group and result in a cancer spectrum different from that of the surrounding populations. The preference of a racially admixed group for classification as white could result in an excess of cancer patients being classified as white. The population groups in southern Louisiana typify the kind of \"human genetics laboratory\" that inbred groups offer to investigators of the genetic aspects of cancer.", "contents": "Genetic considerations in human cancer incidence. Analysis by the methods of genetic demography can offer plausible explanations for the unusual distribution of cancer in an area of high incidence. The important demographic characteristics include inbreeding, founder effect, and racial admixture. Inbreeding would elevate cancer incidence if autosomal recessive genes played a role in cancer etiology. Founder effect would limit this phenomenon to those recessive genes observed in the founding group and result in a cancer spectrum different from that of the surrounding populations. The preference of a racially admixed group for classification as white could result in an excess of cancer patients being classified as white. The population groups in southern Louisiana typify the kind of \"human genetics laboratory\" that inbred groups offer to investigators of the genetic aspects of cancer."}
{"id": "PMID:482586", "title": "The spectrum of hepatic dysfunction in inflammatory bowel disease.", "content": "The spectrum and incidence of liver disease is described among a large series of patients with inflammatory bowel disease. The incidence of significant liver disease identified by the presence of serial biochemical abnormalities of liver function was 8.2 per cent. Transient peri-operative changes in liver function tests are common and usually relate to underlying intra-abdominal sepsis. Percholangitis, sometimes termed portal triaditis, is one of the commoner lesions, and is usually associated with extensive colitis and improves with resection of the underlying bowel disease. Cirrhosis of the liver is an important but uncommon complication and is usually associated with extensive long-standing disease. Stenosing cholangitis and biliary tract carcinoma are both important though rare associations. They are both associated with extensive disease of long-standing, but resection of the underlying inflammatory bowel disease does not necessarily protect the individual from these complications. Although stenosing cholangitis is a diffuse lesion of the biliary tree it is important to exclude strictures of the extra-hepatic biliary tree which may be amenable to surgical correction. Hepatic dysfunction is rarely the sole indication for advising surgery for the underlying bowel disease but the identification of the nature of the hepati- dysfunction provides a rational basis for such a decision and opportunities for the surgical correction of the hepatic lesion itself.", "contents": "The spectrum of hepatic dysfunction in inflammatory bowel disease. The spectrum and incidence of liver disease is described among a large series of patients with inflammatory bowel disease. The incidence of significant liver disease identified by the presence of serial biochemical abnormalities of liver function was 8.2 per cent. Transient peri-operative changes in liver function tests are common and usually relate to underlying intra-abdominal sepsis. Percholangitis, sometimes termed portal triaditis, is one of the commoner lesions, and is usually associated with extensive colitis and improves with resection of the underlying bowel disease. Cirrhosis of the liver is an important but uncommon complication and is usually associated with extensive long-standing disease. Stenosing cholangitis and biliary tract carcinoma are both important though rare associations. They are both associated with extensive disease of long-standing, but resection of the underlying inflammatory bowel disease does not necessarily protect the individual from these complications. Although stenosing cholangitis is a diffuse lesion of the biliary tree it is important to exclude strictures of the extra-hepatic biliary tree which may be amenable to surgical correction. Hepatic dysfunction is rarely the sole indication for advising surgery for the underlying bowel disease but the identification of the nature of the hepati- dysfunction provides a rational basis for such a decision and opportunities for the surgical correction of the hepatic lesion itself."}
{"id": "PMID:482587", "title": "Liver disease as presenting manifestation of Hodgkin's disease.", "content": "Six patients who were referred to the liver unit on account of jaundice are described. A different initial diagnosis has been made in each case, these being fulminant hepatic failure, severe hepatitis with renal failure, toxoplasma hepatitis, extrahepatic obstruction, sclerosing cholangitis, and liver abscess. After delays of four weeks to 12 months from the time of first symptoms all six patients were eventually found to have advanced Hodgkin's disease (stage 4). In four patients the diagnosis was made during life, but in two only at autopsy. In four lymphoma tissue was finally demonstrable in the liver, but in two liver biopsy showed only minor non-specific changes despite grossly abnormal liver function tests. Three of the six patients were treated with chemotherapy, and two of these recovered sufficiently to leave hospital. With the encouraging survival figures now being obtained in Hodgkin's disease, an awareness of the varied hepatic manifestations of the disease may allow treatment to be instituted at an earlier stage.", "contents": "Liver disease as presenting manifestation of Hodgkin's disease. Six patients who were referred to the liver unit on account of jaundice are described. A different initial diagnosis has been made in each case, these being fulminant hepatic failure, severe hepatitis with renal failure, toxoplasma hepatitis, extrahepatic obstruction, sclerosing cholangitis, and liver abscess. After delays of four weeks to 12 months from the time of first symptoms all six patients were eventually found to have advanced Hodgkin's disease (stage 4). In four patients the diagnosis was made during life, but in two only at autopsy. In four lymphoma tissue was finally demonstrable in the liver, but in two liver biopsy showed only minor non-specific changes despite grossly abnormal liver function tests. Three of the six patients were treated with chemotherapy, and two of these recovered sufficiently to leave hospital. With the encouraging survival figures now being obtained in Hodgkin's disease, an awareness of the varied hepatic manifestations of the disease may allow treatment to be instituted at an earlier stage."}
{"id": "PMID:482588", "title": "Angio-immunoblastic lymphadenopathy: report of ten cases and review of the literature.", "content": "Angio-immunoblastic lymphadenopathy (AIL) is a disease of unknown aetiology and pathogenesis. It has features of hyperimmunity, immune deficiency and can behave like a malignant lymphoma. An underlying abnormality of T-cell regulatory function has been proposed. We report ten patients with AIL followed prospectively and review 200 cases from the literature. As well as showing the typical features described in previous retrospective series namely: constitutional symptoms, generalized lymphadenopathy, hepatosplenomegaly, skin eruptions, hypergammaglobulinaemia and characteristic lymph node histology; four patients had oedema with ascites or pleural effusions associated with hyponatraemia and hypoalbuminaemia. We have also observed low free thyroxine indices in three patients with elevated TSH levels in two, but without clinical features of hypothyroidism. Seven of the patients in this study were treated with prednisone and cyclophosphamide. Three achieved complete remission but only one patient has survived longer than two years. Failure to achieve complete remission has been associated with a 90 per cent mortality within one year of the onset of disease in the reports reviewed. Corticosteroids alone have produced only a 40 per cent rate of remission. The management of poor responders, non-responders and many relapse cases in unsatisfactory. More instensive chemotherapy is very hazardous, increasing the already high risk of severe infections, and is often unsuccessful. Histological features recently reported to represent co-existent immunoblastic lymphoma at diagnosis were recognized in three of our patients, two of which went on to develop definite lymphoma.", "contents": "Angio-immunoblastic lymphadenopathy: report of ten cases and review of the literature. Angio-immunoblastic lymphadenopathy (AIL) is a disease of unknown aetiology and pathogenesis. It has features of hyperimmunity, immune deficiency and can behave like a malignant lymphoma. An underlying abnormality of T-cell regulatory function has been proposed. We report ten patients with AIL followed prospectively and review 200 cases from the literature. As well as showing the typical features described in previous retrospective series namely: constitutional symptoms, generalized lymphadenopathy, hepatosplenomegaly, skin eruptions, hypergammaglobulinaemia and characteristic lymph node histology; four patients had oedema with ascites or pleural effusions associated with hyponatraemia and hypoalbuminaemia. We have also observed low free thyroxine indices in three patients with elevated TSH levels in two, but without clinical features of hypothyroidism. Seven of the patients in this study were treated with prednisone and cyclophosphamide. Three achieved complete remission but only one patient has survived longer than two years. Failure to achieve complete remission has been associated with a 90 per cent mortality within one year of the onset of disease in the reports reviewed. Corticosteroids alone have produced only a 40 per cent rate of remission. The management of poor responders, non-responders and many relapse cases in unsatisfactory. More instensive chemotherapy is very hazardous, increasing the already high risk of severe infections, and is often unsuccessful. Histological features recently reported to represent co-existent immunoblastic lymphoma at diagnosis were recognized in three of our patients, two of which went on to develop definite lymphoma."}
{"id": "PMID:482589", "title": "Cerebral complications in the treatment of accelerated hypertension.", "content": "The malignant phase of hypertension is invariably fatal unless treated, and rapid reduction of arterial pressure is thought to be the treatment of choice. Ten patients with accelerated hypertension are described in whom abnormal neurological signs developed following the rapid reduction of arterial pressure. Three patients died without recovering from the neurological damage. A fourth died of an unrelated cause a month later. Areas of ischaemic damage were found in the brains of three of these cases. Of the six survivors, four were left with some permanent neurological disability. It is likely that these changes resulted from the inability of the cerebral circulation in patients with severe hypertension to autoregulate blood flow to the brain, so that a rapid reduction in arterial pressure led to ischaemia, especially of the watershed areas of the brain. Cerebrovascular autoregulation is likely to be compromized in patients with cerebral oedema, stenosis of major cranial vessels or in those patients with long-standing severe hypertension. It is suggested that the blood pressure in patients with accelerated hypertension should be lowered gently over a period of several hours or even days in order to allow time for the cerebrovascular autoregulatory mechanisms to recover.", "contents": "Cerebral complications in the treatment of accelerated hypertension. The malignant phase of hypertension is invariably fatal unless treated, and rapid reduction of arterial pressure is thought to be the treatment of choice. Ten patients with accelerated hypertension are described in whom abnormal neurological signs developed following the rapid reduction of arterial pressure. Three patients died without recovering from the neurological damage. A fourth died of an unrelated cause a month later. Areas of ischaemic damage were found in the brains of three of these cases. Of the six survivors, four were left with some permanent neurological disability. It is likely that these changes resulted from the inability of the cerebral circulation in patients with severe hypertension to autoregulate blood flow to the brain, so that a rapid reduction in arterial pressure led to ischaemia, especially of the watershed areas of the brain. Cerebrovascular autoregulation is likely to be compromized in patients with cerebral oedema, stenosis of major cranial vessels or in those patients with long-standing severe hypertension. It is suggested that the blood pressure in patients with accelerated hypertension should be lowered gently over a period of several hours or even days in order to allow time for the cerebrovascular autoregulatory mechanisms to recover."}
{"id": "PMID:482590", "title": "Chronic subdural haematoma.", "content": "A retrospective case note survey of chronic subdural haematomata was carried out in an attempt to throw some light on the difficulties encountered in clinical diagnosis. The combination of raised intracranial pressure headache, fluctuating drowsiness and mild hemiparesis, although highly suggestive of subdural haematoma, is not always encountered, and epilepsy, aphasia, hemianopia and dense hemiplegia can all occur contrary to 'text book' descriptions. Head injury or other aetiological factors are commonly absent. The presentation may mimic tumour, dementia, cerebrovascular accident or subarachnoid haemorrhage. Non-invasive investigations may yield false negative results, although in the case of radionucleide scanning and computerized axial tomography the reliability is approaching 90 per cent. The diagnosis will, however, remain an unexpected finding at angiography in a percentage of cases.", "contents": "Chronic subdural haematoma. A retrospective case note survey of chronic subdural haematomata was carried out in an attempt to throw some light on the difficulties encountered in clinical diagnosis. The combination of raised intracranial pressure headache, fluctuating drowsiness and mild hemiparesis, although highly suggestive of subdural haematoma, is not always encountered, and epilepsy, aphasia, hemianopia and dense hemiplegia can all occur contrary to 'text book' descriptions. Head injury or other aetiological factors are commonly absent. The presentation may mimic tumour, dementia, cerebrovascular accident or subarachnoid haemorrhage. Non-invasive investigations may yield false negative results, although in the case of radionucleide scanning and computerized axial tomography the reliability is approaching 90 per cent. The diagnosis will, however, remain an unexpected finding at angiography in a percentage of cases."}
{"id": "PMID:482591", "title": "Dissecting aortic aneurysms: a clinicopathological study. I. Clinical and gross pathological findings.", "content": "One hundred and seventy-one patients with dissecting aneurysm seen between 1951 and 1976 at three hospitals in Manchester were studied. There were 60 proximal dissections, 80 distal dissections, 10 abdominal dissections and in 21 the site of origin was uncertain. Pain was the major symptom in 88 per cent of patients; radiation of pain to the interscapular region was much more common in distal dissections. Systemic hypertension was present in 77 per cent, being commoner in distal dissections (83 per cent) than in proximal dissections (70 per cent). Aortic incompetence, hemiplegia and shock were all more common in proximal dissections. Post-mortem examination was performed in 125 patients. Eighty-four per cent of proximal dissections had ruptured, 74 per cent into the pericardium and five per cent into the left pleural cavity. Seventy per cent of distal dissections had ruptured, 11 per cent into the pericardium and 41 per cent into the left pleural cavity. The extent of the dissection was analysed, and it was shown that 25 per cent of distal dissections had extended proximally into the ascending aorta and arch. This implies that diagnosis of the site of origin of dissection from clinical signs and the plain chest-radiograph is inaccurate. Aortography is required for precise assessment. Since treatment often varies with the site of dissection, aortography should be performed in most patients surviving the first few hours. Attention is drawn to the frequency (10.4 per cent) of multiple aortic lesions, and to the occasional aetiological significance of giant-cell arteritis, and, possibly, hypothyroidism.", "contents": "Dissecting aortic aneurysms: a clinicopathological study. I. Clinical and gross pathological findings. One hundred and seventy-one patients with dissecting aneurysm seen between 1951 and 1976 at three hospitals in Manchester were studied. There were 60 proximal dissections, 80 distal dissections, 10 abdominal dissections and in 21 the site of origin was uncertain. Pain was the major symptom in 88 per cent of patients; radiation of pain to the interscapular region was much more common in distal dissections. Systemic hypertension was present in 77 per cent, being commoner in distal dissections (83 per cent) than in proximal dissections (70 per cent). Aortic incompetence, hemiplegia and shock were all more common in proximal dissections. Post-mortem examination was performed in 125 patients. Eighty-four per cent of proximal dissections had ruptured, 74 per cent into the pericardium and five per cent into the left pleural cavity. Seventy per cent of distal dissections had ruptured, 11 per cent into the pericardium and 41 per cent into the left pleural cavity. The extent of the dissection was analysed, and it was shown that 25 per cent of distal dissections had extended proximally into the ascending aorta and arch. This implies that diagnosis of the site of origin of dissection from clinical signs and the plain chest-radiograph is inaccurate. Aortography is required for precise assessment. Since treatment often varies with the site of dissection, aortography should be performed in most patients surviving the first few hours. Attention is drawn to the frequency (10.4 per cent) of multiple aortic lesions, and to the occasional aetiological significance of giant-cell arteritis, and, possibly, hypothyroidism."}
{"id": "PMID:482592", "title": "Dissecting aortic aneurysms: a clinicopathological study. II. Histopathology of the aorta.", "content": "The aortas from 83 patients with dissecting aneurysm were studied histologically. There were two examples of giant-cell arteritis, one of non-specific arteritis and one of syphilis. Apart from these, no constant feature was found in the cases of dissection when compared with 20 controls. In particular there was no difference in the grades of cystic medial necrosis, medionecrosis and fibrosis. Focal fragmentation of elastica was present in the majority of dissections (63 per cent) but similar abnormalities were seen in most controls (85 per cent). The vasa vasorum showed no special features in dissection. Intimal fibrosis and intimal longitudinal muscle were present in both groups studied. Sixteen cases showed 'old' dissections as manifest by either granulation tissue or fibrosis. The histopathological features of the four cases diagnosed clinically as having Marfan's syndrome did not differ from controls.", "contents": "Dissecting aortic aneurysms: a clinicopathological study. II. Histopathology of the aorta. The aortas from 83 patients with dissecting aneurysm were studied histologically. There were two examples of giant-cell arteritis, one of non-specific arteritis and one of syphilis. Apart from these, no constant feature was found in the cases of dissection when compared with 20 controls. In particular there was no difference in the grades of cystic medial necrosis, medionecrosis and fibrosis. Focal fragmentation of elastica was present in the majority of dissections (63 per cent) but similar abnormalities were seen in most controls (85 per cent). The vasa vasorum showed no special features in dissection. Intimal fibrosis and intimal longitudinal muscle were present in both groups studied. Sixteen cases showed 'old' dissections as manifest by either granulation tissue or fibrosis. The histopathological features of the four cases diagnosed clinically as having Marfan's syndrome did not differ from controls."}
{"id": "PMID:482593", "title": "Clinico-pathological features of the nephrotic syndrome in South African children.", "content": "One hundred and thirty children of whom 74 were Africans and 56 Indians with contrasting clinicopathological patterns of the nephrotic syndrome are described. Eighty-six per cent of African children had obvious structural glomerular lesions which were associated with unresponsiveness to steroids while 75 per cent of Indians had minimal change nephrotic syndrome which was steroid responsive. The treatment history of a further 41 unbiopsied children with nephrotic syndrome (nine Africans, 32 Indians) support and emphasize this difference. Extramembranous and a tropical variety of extramembranous (36.5 per cent) together with proliferative (20.2 per cent) lesions accounted for most of the histological types in African children. The disease in Indian children was similar to that in other countries in age of onset, sex distribution, frequency of histological types and steroid responsiveness although there was a preponderance of frequent relapsers (69 per cent). Africans differed from children in other continents in the frequency of histological categories (therefore in steroid responsiveness) and occasionally in clinical behaviour. They also differed from children in tropical Africa in a lower incidence of the disease, male dominance and absence of malarial nephropathy. The aetiology of nephrotic syndrome in nearly all the children remains unidentified.", "contents": "Clinico-pathological features of the nephrotic syndrome in South African children. One hundred and thirty children of whom 74 were Africans and 56 Indians with contrasting clinicopathological patterns of the nephrotic syndrome are described. Eighty-six per cent of African children had obvious structural glomerular lesions which were associated with unresponsiveness to steroids while 75 per cent of Indians had minimal change nephrotic syndrome which was steroid responsive. The treatment history of a further 41 unbiopsied children with nephrotic syndrome (nine Africans, 32 Indians) support and emphasize this difference. Extramembranous and a tropical variety of extramembranous (36.5 per cent) together with proliferative (20.2 per cent) lesions accounted for most of the histological types in African children. The disease in Indian children was similar to that in other countries in age of onset, sex distribution, frequency of histological types and steroid responsiveness although there was a preponderance of frequent relapsers (69 per cent). Africans differed from children in other continents in the frequency of histological categories (therefore in steroid responsiveness) and occasionally in clinical behaviour. They also differed from children in tropical Africa in a lower incidence of the disease, male dominance and absence of malarial nephropathy. The aetiology of nephrotic syndrome in nearly all the children remains unidentified."}
{"id": "PMID:482594", "title": "Pericarditis and inflammatory bowel disease.", "content": "Pericarditis is an uncommon but important complication of inflammatory bowel disease. Five new cases are described and the published literature on nine other cases is reviewed. The pericarditis occurred during an active phase of the colitis in all our cases and was associated with other extra-colonic manifestations of colitis in three. No other cause for the pericarditis was identified and in all five cases it responded promptly to the administration of corticosteroid drugs.", "contents": "Pericarditis and inflammatory bowel disease. Pericarditis is an uncommon but important complication of inflammatory bowel disease. Five new cases are described and the published literature on nine other cases is reviewed. The pericarditis occurred during an active phase of the colitis in all our cases and was associated with other extra-colonic manifestations of colitis in three. No other cause for the pericarditis was identified and in all five cases it responded promptly to the administration of corticosteroid drugs."}
{"id": "PMID:482595", "title": "Acute post-streptococcal glomerulonephritis in adults: a long-term study.", "content": "The long-term outcome after acute post-streptococcal glomerulonephritis was studied in 57 patients (52 aged 16 or over) followed for a period of one to 14 years (mean seven years). All patients presented with hypertension, haematuria and proteinuria. The antistreptolysin-0 titre was raised or the serum complement was low in all cases at the initial episode. All patients had histological evidence of a diffuse proliferative and exudative glomerulonephritis at onset. Follow-up renal biopsy was performed in 33 patients; in 18 patients this was carried out five years or more after the initial illness. Five patients died beyond two years, only two having had abnormal renal function at the time or death. Four patients were found to be mildly hypertensive without other clinical abnormalities. Eleven patients had proteinuria, haematuria or abnormal renal function; in three of these repeat renal biopsy was normal, incomplete resolution was reported in five, obsolescent glomeruli in one, and two others were not biopsied. No patient who had normal renal function at the time of follow-up had abnormal renal histology on biopsy. Obsolescent glomeruli were present in two other biopsies in association with evidence of incomplete resolution. It was concluded that the majority of patients with acute PSGN have a good prognosis. Histological resolution of the renal lesion may not occur for nine years.", "contents": "Acute post-streptococcal glomerulonephritis in adults: a long-term study. The long-term outcome after acute post-streptococcal glomerulonephritis was studied in 57 patients (52 aged 16 or over) followed for a period of one to 14 years (mean seven years). All patients presented with hypertension, haematuria and proteinuria. The antistreptolysin-0 titre was raised or the serum complement was low in all cases at the initial episode. All patients had histological evidence of a diffuse proliferative and exudative glomerulonephritis at onset. Follow-up renal biopsy was performed in 33 patients; in 18 patients this was carried out five years or more after the initial illness. Five patients died beyond two years, only two having had abnormal renal function at the time or death. Four patients were found to be mildly hypertensive without other clinical abnormalities. Eleven patients had proteinuria, haematuria or abnormal renal function; in three of these repeat renal biopsy was normal, incomplete resolution was reported in five, obsolescent glomeruli in one, and two others were not biopsied. No patient who had normal renal function at the time of follow-up had abnormal renal histology on biopsy. Obsolescent glomeruli were present in two other biopsies in association with evidence of incomplete resolution. It was concluded that the majority of patients with acute PSGN have a good prognosis. Histological resolution of the renal lesion may not occur for nine years."}
{"id": "PMID:482617", "title": "Cancer, the public, and the radiographer.", "content": "All radiographers can help bring about more cancer cures. In this context one's specialty--diagnosis or therapy--is less important than the fact that, because radiographers are seen by the public to be knowledgeable about health matters, they can exert an influence on how people think about the cancers. Why public attitudes to cancer adversely affect the number of patients cured and how radiographers might help improve the present lamentable situation is the subject of this paper.", "contents": "Cancer, the public, and the radiographer. All radiographers can help bring about more cancer cures. In this context one's specialty--diagnosis or therapy--is less important than the fact that, because radiographers are seen by the public to be knowledgeable about health matters, they can exert an influence on how people think about the cancers. Why public attitudes to cancer adversely affect the number of patients cured and how radiographers might help improve the present lamentable situation is the subject of this paper."}
{"id": "PMID:482621", "title": "[Diagnostic value of sonography of the bile ducts (author's transl)].", "content": "In obstructive biliary disease sonography proves to have a high diagnostic informative value. Dilatation of intra-and extrahepatic bile ducts is documented sonographically. In the majority of cases, analysis of sonographic findings will clarify the cause of biliary obstruction like cholecysto-cholangiolithiasis, carcinoma of the head of the pancreas, or various neoplastic disease in the area of the liver hilus. The obtained information is independent from contraindications like contrast medium allergies and severe parenchymal liver disease with impaired biliary secretion of contrast media.", "contents": "[Diagnostic value of sonography of the bile ducts (author's transl)]. In obstructive biliary disease sonography proves to have a high diagnostic informative value. Dilatation of intra-and extrahepatic bile ducts is documented sonographically. In the majority of cases, analysis of sonographic findings will clarify the cause of biliary obstruction like cholecysto-cholangiolithiasis, carcinoma of the head of the pancreas, or various neoplastic disease in the area of the liver hilus. The obtained information is independent from contraindications like contrast medium allergies and severe parenchymal liver disease with impaired biliary secretion of contrast media."}
{"id": "PMID:482622", "title": "[Sonograhic-radiologic evaluation of obstructive jaundice (author's transl)].", "content": "Retrospective evaluation of indication and technical procedure and its value in sonography of 110 patients with jaundice. A correct diagnosis (by ultrasound) of hepatogenic jaundice was made in 36/38 patients (94%) and of obstructive jaundice in 69/72 patients (96%). In additional 31/72 patients (43%) ultrasound gave important diagnostic clues by demonstrating dilated bile ducts as well as the location of obstruction. For verification and for the demonstration of morphologic changes, sonographically guided fine needle aspiration puncture, percutaneous transhepatic cholangiography with a fine puncture neede (Chiba-needle) and ERCP are indicated.", "contents": "[Sonograhic-radiologic evaluation of obstructive jaundice (author's transl)]. Retrospective evaluation of indication and technical procedure and its value in sonography of 110 patients with jaundice. A correct diagnosis (by ultrasound) of hepatogenic jaundice was made in 36/38 patients (94%) and of obstructive jaundice in 69/72 patients (96%). In additional 31/72 patients (43%) ultrasound gave important diagnostic clues by demonstrating dilated bile ducts as well as the location of obstruction. For verification and for the demonstration of morphologic changes, sonographically guided fine needle aspiration puncture, percutaneous transhepatic cholangiography with a fine puncture neede (Chiba-needle) and ERCP are indicated."}
{"id": "PMID:482623", "title": "[Improved diagnosis in percutaneous transhepatic cholangiography (PTC) (author's transl)].", "content": "PTC is a good radiographic method in the diagnosis of cholestasis. Complications are rare, primarily due to the ultra-thin Chiba needle. For penetrating the liver we recommend to keep a distance of at least 4 cm between needle and spine (measured on the monitor). Our experience has shown routine liver tomography to be very helpful. Furthermore, delayed radiography can show extrahepatic bile ducts not seen on initial films.", "contents": "[Improved diagnosis in percutaneous transhepatic cholangiography (PTC) (author's transl)]. PTC is a good radiographic method in the diagnosis of cholestasis. Complications are rare, primarily due to the ultra-thin Chiba needle. For penetrating the liver we recommend to keep a distance of at least 4 cm between needle and spine (measured on the monitor). Our experience has shown routine liver tomography to be very helpful. Furthermore, delayed radiography can show extrahepatic bile ducts not seen on initial films."}
{"id": "PMID:482624", "title": "[Direct cholangiography: endoscopic-retrograde or percutaneous transhepatic? (author's transl)].", "content": "Indications, success-rate and disadvantages of endoscopic retrograde (n = 928) and percutaneous transhepatic cholangiography (n approximately 360) at the Surgical and Medical University Clinics of Freiburg/German are discussed. Direct cholegraphy is the method of choice in cases of cholestasis of unknown origin. Endoscopic retrograde procedures usually preceed the percutaneous transhepatic approach. PTC either complementary or decisive when ERCP has failed. Therapeutic possibilities of both methods are not yet totally utilized.", "contents": "[Direct cholangiography: endoscopic-retrograde or percutaneous transhepatic? (author's transl)]. Indications, success-rate and disadvantages of endoscopic retrograde (n = 928) and percutaneous transhepatic cholangiography (n approximately 360) at the Surgical and Medical University Clinics of Freiburg/German are discussed. Direct cholegraphy is the method of choice in cases of cholestasis of unknown origin. Endoscopic retrograde procedures usually preceed the percutaneous transhepatic approach. PTC either complementary or decisive when ERCP has failed. Therapeutic possibilities of both methods are not yet totally utilized."}
{"id": "PMID:482625", "title": "[Percutaneous transhepatic drainage of the biliary tract for preoperative and palliative therapy of obstructive biliary lesions (author's transl)].", "content": "The percutaneous transhepatic catheterisation is an important method in the management of obstructive jaundice. The results of the percutaneous transhepatic drainage - external and internal - in 14 patients are reported. The technique is described and the difficulties, complications, indications and contraindications are discussed. The value of this procedure is the temporary decompression of the obstructed biliary tract prior to radical surgery or as a palliation in patients with non-operable tumors; the risk of the procedure is low.", "contents": "[Percutaneous transhepatic drainage of the biliary tract for preoperative and palliative therapy of obstructive biliary lesions (author's transl)]. The percutaneous transhepatic catheterisation is an important method in the management of obstructive jaundice. The results of the percutaneous transhepatic drainage - external and internal - in 14 patients are reported. The technique is described and the difficulties, complications, indications and contraindications are discussed. The value of this procedure is the temporary decompression of the obstructed biliary tract prior to radical surgery or as a palliation in patients with non-operable tumors; the risk of the procedure is low."}
{"id": "PMID:482654", "title": "Effects of the host matrix on the 57Fe-species produced through EC-decay in 57Co-labelled tris(phenanthroline) cobalt (II) perchlorate.", "content": "Emission M\u00f6ssbauer spectroscopic studies of 57Co-labelled [Co(phen)3]clO4)2 in host matrices [M(II)(phen)3](ClO4)2(M=Co, Fe, and Ni) indicate that the relative intensities of the anomalous species produced through the EC-decay depend on the kind of the host matrix. The largest intensity was observed with the cobalt (II) matrix, and the smallest with the iron (II) matrix. Emission spectra of 57Co-labelled [Co(2-CH3-phen)3](ClO4)2 2H2O in the matrix of [Fe(2-CH3-phen)3](ClO4)2 were also studied. The high-spin state (5T2) was predominantly observed at 4.2 K in the emission spectrum, while the low-spin state (1A1) was mainly observed in the absorption spectrum at 78 K. The results are discussed in terms of the stability of the lattice.", "contents": "Effects of the host matrix on the 57Fe-species produced through EC-decay in 57Co-labelled tris(phenanthroline) cobalt (II) perchlorate. Emission M\u00f6ssbauer spectroscopic studies of 57Co-labelled [Co(phen)3]clO4)2 in host matrices [M(II)(phen)3](ClO4)2(M=Co, Fe, and Ni) indicate that the relative intensities of the anomalous species produced through the EC-decay depend on the kind of the host matrix. The largest intensity was observed with the cobalt (II) matrix, and the smallest with the iron (II) matrix. Emission spectra of 57Co-labelled [Co(2-CH3-phen)3](ClO4)2 2H2O in the matrix of [Fe(2-CH3-phen)3](ClO4)2 were also studied. The high-spin state (5T2) was predominantly observed at 4.2 K in the emission spectrum, while the low-spin state (1A1) was mainly observed in the absorption spectrum at 78 K. The results are discussed in terms of the stability of the lattice."}
{"id": "PMID:482655", "title": "[Accumulation mechanism of 111In in malignant tumor (author's transl)].", "content": "In order to clarify the accumulation mechanism of 111In in maligant tumor, subcellular distribution of 111In was quantitatively determined. Buffalo rats bearing Morris hepatoma 7316A were injected intraperitoneally 111In-chloride and tumor tissues were removed 24 hours later. Subcellular fractionation of tumor tissues were carried out according to the method of C. de Duve, et al. and radioactivity of each fraction was counted. Most of the total radioactivity was distributed among the soluble, nuclear and lysosomal fractions. On account of its low protein content, the relative specific radioactivity was the highest in the lysosomal fraction. The lysosomal fraction was solubilized gradually and the resultant stepwise release of 111In and acid phosphatase activity were measured. There was a close relationship between them. From these results it was concluded that 111In accumulated especially in the lysosomes. In the electron micrography the tumor lysosomes had already engulfed many foreign materials, so that the lysosomal function would be depressed.", "contents": "[Accumulation mechanism of 111In in malignant tumor (author's transl)]. In order to clarify the accumulation mechanism of 111In in maligant tumor, subcellular distribution of 111In was quantitatively determined. Buffalo rats bearing Morris hepatoma 7316A were injected intraperitoneally 111In-chloride and tumor tissues were removed 24 hours later. Subcellular fractionation of tumor tissues were carried out according to the method of C. de Duve, et al. and radioactivity of each fraction was counted. Most of the total radioactivity was distributed among the soluble, nuclear and lysosomal fractions. On account of its low protein content, the relative specific radioactivity was the highest in the lysosomal fraction. The lysosomal fraction was solubilized gradually and the resultant stepwise release of 111In and acid phosphatase activity were measured. There was a close relationship between them. From these results it was concluded that 111In accumulated especially in the lysosomes. In the electron micrography the tumor lysosomes had already engulfed many foreign materials, so that the lysosomal function would be depressed."}
{"id": "PMID:482664", "title": "[Re-operations for biliary disorders in cholecystectomized patients].", "content": "A total of 125 cholecystectomized patients are analized, that underwent new surgery for major biliary disturbances. From this study were excluded patients in whom re-interventions were performed for immediate postoperative complications, for surgical accidents, those in whom the primary intervention involved the major biliary pathway, as well as those in whom reintervention was necessary because of other disturbances. 72,8% of the patients in whom reinterventions had to be performed presented one or more obstacles in the major biliary pathway in the authors' opinion coexisted with the initial cholelithiasis. The authors consider that reinterventions should be performed as early as possible, interpretation of the disturbances sequellae of cholecystectomy representing a futile temporization and making more severe the evolution of the patient's condition.", "contents": "[Re-operations for biliary disorders in cholecystectomized patients]. A total of 125 cholecystectomized patients are analized, that underwent new surgery for major biliary disturbances. From this study were excluded patients in whom re-interventions were performed for immediate postoperative complications, for surgical accidents, those in whom the primary intervention involved the major biliary pathway, as well as those in whom reintervention was necessary because of other disturbances. 72,8% of the patients in whom reinterventions had to be performed presented one or more obstacles in the major biliary pathway in the authors' opinion coexisted with the initial cholelithiasis. The authors consider that reinterventions should be performed as early as possible, interpretation of the disturbances sequellae of cholecystectomy representing a futile temporization and making more severe the evolution of the patient's condition."}
{"id": "PMID:482665", "title": "[Chronic pancreatitis of biliary origin. Certain aspects of diagnosis and surgical treatment].", "content": "Chronic pancreatitis of biliary origin, frequently localized in the cephalic segment, are generally dependent on the etiopathogeny of the biliary affection, the evolution of which is complicated by their presence. They have a less severe prognosis and in the large majority of cases they are reversible following radical surgical solution of the pathologic biliary component. The study was concerned with 90 cases of which 67 were associated to biliary lithiasis, 9 to non-lithiasic cholecystopathies and 14 were associated to chronic postoperative pancreatitis (of which 6 were lithiasic). The diagnosis were established only during surgey, by coroboration of surgical, cholangiographic and instrumental data. The surgical attitude developed according to results obtained by pre-and intra-operative examination, with the aspect of biliopancreatic lesions, preference being given to radical and decompressive interventions. Simple cholecystectomy was justified in only 27 cases while in 47 other cases it was associated with choledocotomy, in 19 cases with external biliary drainage, with choledocoduodenostomy in 27 cases, with oddian sphincteroplasty in 8 cases, with oddian sphincteroplasty associated to Wirsung duct sphincteroplasty in 5 cases and with choledocojejunostomy in one case. In 2 patients right splanchnicectomy was carried out. The postoperative results were very good in 41 cases, good in 33, mediocre in 13 and unsatisfactory in one case. Two patients died.", "contents": "[Chronic pancreatitis of biliary origin. Certain aspects of diagnosis and surgical treatment]. Chronic pancreatitis of biliary origin, frequently localized in the cephalic segment, are generally dependent on the etiopathogeny of the biliary affection, the evolution of which is complicated by their presence. They have a less severe prognosis and in the large majority of cases they are reversible following radical surgical solution of the pathologic biliary component. The study was concerned with 90 cases of which 67 were associated to biliary lithiasis, 9 to non-lithiasic cholecystopathies and 14 were associated to chronic postoperative pancreatitis (of which 6 were lithiasic). The diagnosis were established only during surgey, by coroboration of surgical, cholangiographic and instrumental data. The surgical attitude developed according to results obtained by pre-and intra-operative examination, with the aspect of biliopancreatic lesions, preference being given to radical and decompressive interventions. Simple cholecystectomy was justified in only 27 cases while in 47 other cases it was associated with choledocotomy, in 19 cases with external biliary drainage, with choledocoduodenostomy in 27 cases, with oddian sphincteroplasty in 8 cases, with oddian sphincteroplasty associated to Wirsung duct sphincteroplasty in 5 cases and with choledocojejunostomy in one case. In 2 patients right splanchnicectomy was carried out. The postoperative results were very good in 41 cases, good in 33, mediocre in 13 and unsatisfactory in one case. Two patients died."}
{"id": "PMID:482667", "title": "[Primary renal actinomycosis].", "content": "A case of renal actinomycosis, treated by nephrectomy, in a 41 year-old male, is reported. The clinical diagnosis, to which urography and renal arteriography lent support, was renal anthrax. The pus being sterile on conventional seeding, the investigations were directed towards the finding of characteristic mycelium. Five months after nephrectomy a cerebral abscess developed and the patient died. Although in the cerebral pus only B. proteus and coagulase-positive staphylococci were found, the actinomycotic etiology cannot be excluded since the specific granules were not looked for.", "contents": "[Primary renal actinomycosis]. A case of renal actinomycosis, treated by nephrectomy, in a 41 year-old male, is reported. The clinical diagnosis, to which urography and renal arteriography lent support, was renal anthrax. The pus being sterile on conventional seeding, the investigations were directed towards the finding of characteristic mycelium. Five months after nephrectomy a cerebral abscess developed and the patient died. Although in the cerebral pus only B. proteus and coagulase-positive staphylococci were found, the actinomycotic etiology cannot be excluded since the specific granules were not looked for."}
{"id": "PMID:482668", "title": "[Surgical aspects of malignant plasmacytoma].", "content": "The case is presented of a patient presenting with a solitary plasmocytoma of the cecum (the 5-th case reported in the world medical literature), for which right hemicolectomy was performed. A second observation is presented, concerning an enteromesenteric infarction that developed on the background of multiple myeloma. Segmental enterectomy was performed.", "contents": "[Surgical aspects of malignant plasmacytoma]. The case is presented of a patient presenting with a solitary plasmocytoma of the cecum (the 5-th case reported in the world medical literature), for which right hemicolectomy was performed. A second observation is presented, concerning an enteromesenteric infarction that developed on the background of multiple myeloma. Segmental enterectomy was performed."}
{"id": "PMID:482670", "title": "[Ulcer of the transverse colon].", "content": "This very rare affection of unknown origin, achieves a macroscopic and microscopic aspect similar to that described by Cruveillhier at the level of the stomach. The authors present a case of ulcer located on the transverse colon, at the free margin, with stenosing evolution and coexisting with duodenal ulceration of chronic character. The patient also had portal hypertension in the third stage and arteriopathy that had been diagnosed previously.", "contents": "[Ulcer of the transverse colon]. This very rare affection of unknown origin, achieves a macroscopic and microscopic aspect similar to that described by Cruveillhier at the level of the stomach. The authors present a case of ulcer located on the transverse colon, at the free margin, with stenosing evolution and coexisting with duodenal ulceration of chronic character. The patient also had portal hypertension in the third stage and arteriopathy that had been diagnosed previously."}
{"id": "PMID:482671", "title": "[Ketalar pivot anesthesia in cortisone-dependent patients].", "content": "On the basis of results obtained in a series of 6 patients with corticoid-dependency in whom 7 surgical interventions have been carried out the authors present the anesthesia technique on Ketalar pivot according to the following protocol: premedication with Diazepam and atropin; induction of anesthesia with Ketalar (2 mg/kg of body weight) and Pavulon 0,1 mg/kg of body weight); maintenance with Ketalar (1 mg/kg of body weight) at 10-15 minutes, and NO2/O2 4/2 at 1 minute. The cardiovascular stability was satisfactory. No fall in the value of blood pressure was noted.", "contents": "[Ketalar pivot anesthesia in cortisone-dependent patients]. On the basis of results obtained in a series of 6 patients with corticoid-dependency in whom 7 surgical interventions have been carried out the authors present the anesthesia technique on Ketalar pivot according to the following protocol: premedication with Diazepam and atropin; induction of anesthesia with Ketalar (2 mg/kg of body weight) and Pavulon 0,1 mg/kg of body weight); maintenance with Ketalar (1 mg/kg of body weight) at 10-15 minutes, and NO2/O2 4/2 at 1 minute. The cardiovascular stability was satisfactory. No fall in the value of blood pressure was noted."}
{"id": "PMID:482716", "title": "[Effect of alloxan in pancreatectomized rats (author's transl)].", "content": "Sensitivty to alloxan of pancreatectomized rats is studied injecting on the seventh day after surgical resection with different dose of monohydrated alloxan. Totally pancreatectomized rats develop a strong diabetes just the same as the control group, but the former present higher glycemias and longer survival.", "contents": "[Effect of alloxan in pancreatectomized rats (author's transl)]. Sensitivty to alloxan of pancreatectomized rats is studied injecting on the seventh day after surgical resection with different dose of monohydrated alloxan. Totally pancreatectomized rats develop a strong diabetes just the same as the control group, but the former present higher glycemias and longer survival."}
{"id": "PMID:482717", "title": "The effect of human placental lactogen upon the metabolism of rat and human adipose tissue.", "content": "The metabolic effects of human placental lactogen (HPL) on rat and human white fat were tested in vitro. When tested against rat tissue, HPL resembled insulin in stimulating uptake of glucose and incorporation of [14C] glucose into CO2, triglyceride and glycogen, but differed from insulin in stimulating glycerol release and in failing to stimulate the incorporation of [14C] The stimulation of [14C] glucose incorporation and the inhibition of glycerol release by insulin were antagonized by HPL. The effects of HPL on human white fat resembled those on rat white fat,except that glycerol release was not stimulated in human tissue. The possible role of HPL in causing the diabetogenic stress of pregnancy is discussed in the light of these findings.", "contents": "The effect of human placental lactogen upon the metabolism of rat and human adipose tissue. The metabolic effects of human placental lactogen (HPL) on rat and human white fat were tested in vitro. When tested against rat tissue, HPL resembled insulin in stimulating uptake of glucose and incorporation of [14C] glucose into CO2, triglyceride and glycogen, but differed from insulin in stimulating glycerol release and in failing to stimulate the incorporation of [14C] The stimulation of [14C] glucose incorporation and the inhibition of glycerol release by insulin were antagonized by HPL. The effects of HPL on human white fat resembled those on rat white fat,except that glycerol release was not stimulated in human tissue. The possible role of HPL in causing the diabetogenic stress of pregnancy is discussed in the light of these findings."}
{"id": "PMID:482718", "title": "[Effect of intracerebroventricular injection of 6-hydroxydopamine and 5,6-dyhydroxytryptamine on the phasic secretion of luteinizing hormone (author's transl)].", "content": "In ovariectomized rats carrying an estradiol sylastic implant the effect of the intraventricular administration of the serotonin neurotoxin 5,6-dihydroxytryptamine and of the norepinephrine neurotoxin 6-hydroxydopamine on the circadian secretion of the luteinizing hormone (LH) was studied. The decrease of 5-HT after injection of 5,6-dihydroxytryptamine reduced the a.m. and p.m. values and suppressed the phasic rhythm of LH. The diminution of noradrenaline after administration of 6-hydroxydopamine increased the a.m. and did not alter the p.m. values, resulting in a partial abolition of the rhythm.", "contents": "[Effect of intracerebroventricular injection of 6-hydroxydopamine and 5,6-dyhydroxytryptamine on the phasic secretion of luteinizing hormone (author's transl)]. In ovariectomized rats carrying an estradiol sylastic implant the effect of the intraventricular administration of the serotonin neurotoxin 5,6-dihydroxytryptamine and of the norepinephrine neurotoxin 6-hydroxydopamine on the circadian secretion of the luteinizing hormone (LH) was studied. The decrease of 5-HT after injection of 5,6-dihydroxytryptamine reduced the a.m. and p.m. values and suppressed the phasic rhythm of LH. The diminution of noradrenaline after administration of 6-hydroxydopamine increased the a.m. and did not alter the p.m. values, resulting in a partial abolition of the rhythm."}
{"id": "PMID:482719", "title": "Single alternation with water reward:effects of sodium amobarbital on two strains of rats selectively bred for high and low emotionality.", "content": "The action of sodium amobarbital is studied during the patterning effect in rats selectively bred for low and high emotionality. The drug disrupts patterned running in the goal section of the alley in Maudsley non-reactive but not in Maudsley reactive rats for the last five trial-pairs. Sodium amobarbital also affects the behavior of these strains in a differential manner, in start and run sections, during the first trial-pair. The results show that a function-related physiological change has taken place in the Maudsley strains of rats.", "contents": "Single alternation with water reward:effects of sodium amobarbital on two strains of rats selectively bred for high and low emotionality. The action of sodium amobarbital is studied during the patterning effect in rats selectively bred for low and high emotionality. The drug disrupts patterned running in the goal section of the alley in Maudsley non-reactive but not in Maudsley reactive rats for the last five trial-pairs. Sodium amobarbital also affects the behavior of these strains in a differential manner, in start and run sections, during the first trial-pair. The results show that a function-related physiological change has taken place in the Maudsley strains of rats."}
{"id": "PMID:482720", "title": "[Sympathetic control on salivary secretion by the parotid gland in rabbit. I. Effects on the unstimulated gland (author's transl)].", "content": "The sympathetic influences on the rabbit unstimulated parotid gland were studied. The experiments were carried out in anaesthetized rabbits with the Stenon aduct cannulated. Direct stimulation of the superior cervical ganglion elicits variable salivary flows. The high amylase content in the saliva points to a sympathetic secretory action upon acinar cells. The administration of alpha-adrenergic blocking agents (dihydroergotamine, phentolamine and phenoxybenzamine) clearly reduces and even abolishes the effect of the sympathetic stimulation upon flow. The administration of a beta-adrenergic blocking agent (propranolol) slightly reduces the sympathetic action. However the amylase activity is greatly reduced. All this suggests that the secretory effects on the fluid fraction should predominantly be alpha-adrenergic while on the secretion of enzymes the beta-receptors should play an important role.", "contents": "[Sympathetic control on salivary secretion by the parotid gland in rabbit. I. Effects on the unstimulated gland (author's transl)]. The sympathetic influences on the rabbit unstimulated parotid gland were studied. The experiments were carried out in anaesthetized rabbits with the Stenon aduct cannulated. Direct stimulation of the superior cervical ganglion elicits variable salivary flows. The high amylase content in the saliva points to a sympathetic secretory action upon acinar cells. The administration of alpha-adrenergic blocking agents (dihydroergotamine, phentolamine and phenoxybenzamine) clearly reduces and even abolishes the effect of the sympathetic stimulation upon flow. The administration of a beta-adrenergic blocking agent (propranolol) slightly reduces the sympathetic action. However the amylase activity is greatly reduced. All this suggests that the secretory effects on the fluid fraction should predominantly be alpha-adrenergic while on the secretion of enzymes the beta-receptors should play an important role."}
{"id": "PMID:482721", "title": "Molecular species of phosphatidylcholine and phosphatidylethanolamine in subcellular membranes from rat liver.", "content": "Four subfractions of phosphatidycholine and phosphyatidylethanolamine according to the degree of unsaturation of their fatty acids have been separated from lipid extracts of microsomes, and inner and outer mitochondrial membranes. The predominant species found in the three membranes contained one saturated and one unsaturated fatty acid. In microsomes completely saturated species of both phosphatidylcholine and phosphatideylethanolamine were practically nonexistent. In outer mitochondrial membranes species with two unsaturated fatty acids were absent. In the inner mitochondrial membranes, however, disaturated species and those with two unsaturated fatty acids were found.", "contents": "Molecular species of phosphatidylcholine and phosphatidylethanolamine in subcellular membranes from rat liver. Four subfractions of phosphatidycholine and phosphyatidylethanolamine according to the degree of unsaturation of their fatty acids have been separated from lipid extracts of microsomes, and inner and outer mitochondrial membranes. The predominant species found in the three membranes contained one saturated and one unsaturated fatty acid. In microsomes completely saturated species of both phosphatidylcholine and phosphatideylethanolamine were practically nonexistent. In outer mitochondrial membranes species with two unsaturated fatty acids were absent. In the inner mitochondrial membranes, however, disaturated species and those with two unsaturated fatty acids were found."}
{"id": "PMID:482722", "title": "A method for the estimation of striated muscle mass in small laboratory animals.", "content": "A method for the determination of the total muscle mass in small experimental animals is presented. Muscle proteins were quantified in the 1 M LiCl-soluble and distilled water-insoluble fraction of the eyeless, brainless, eviscerated and skinned carcass, as compared with a striated muscle sample from the same animal used as standard and processed in the same way as the whole carcass. The non-muscular tissues left in the carcass do not interfere with the final results. The total muscle mass in adult rats was estimated as 42.0 +/- 2.8% of the in vivo weight.", "contents": "A method for the estimation of striated muscle mass in small laboratory animals. A method for the determination of the total muscle mass in small experimental animals is presented. Muscle proteins were quantified in the 1 M LiCl-soluble and distilled water-insoluble fraction of the eyeless, brainless, eviscerated and skinned carcass, as compared with a striated muscle sample from the same animal used as standard and processed in the same way as the whole carcass. The non-muscular tissues left in the carcass do not interfere with the final results. The total muscle mass in adult rats was estimated as 42.0 +/- 2.8% of the in vivo weight."}
{"id": "PMID:482723", "title": "Competitive kinetics of sugar active transport in snail intestine.", "content": "Everted intestinal rings of the snail Cryptomphalus hortensis accumulate labelled sugars against a concentration gradient. The active transport of D-galactose (KT = 3.6 mM) is competitively inhibited by D-glucose (Ki = 8.2 mM) and by 3-0-methylglucose (Ki = 24 mM), but it is not affected by L-arabinose, D-fructose, L-arabinose and D-mannitol penetrate into the tissue at the same rate, they do not develop accumulation gradient, and all of them follow the kinetics of a diffusion process. D-glucose, on the contrary, like galactose, penetrates much more quickly and accumulates against a gradient.", "contents": "Competitive kinetics of sugar active transport in snail intestine. Everted intestinal rings of the snail Cryptomphalus hortensis accumulate labelled sugars against a concentration gradient. The active transport of D-galactose (KT = 3.6 mM) is competitively inhibited by D-glucose (Ki = 8.2 mM) and by 3-0-methylglucose (Ki = 24 mM), but it is not affected by L-arabinose, D-fructose, L-arabinose and D-mannitol penetrate into the tissue at the same rate, they do not develop accumulation gradient, and all of them follow the kinetics of a diffusion process. D-glucose, on the contrary, like galactose, penetrates much more quickly and accumulates against a gradient."}
{"id": "PMID:482728", "title": "[The Federal Rehabilitation Council - a review of the last decade and prospects for the future (author's transl)].", "content": "Ten years have now passed since the foundation of the Federal Rehabilitation Council. Its work has both reflected and guided the development of rehabilitation in the Federal Republic of Germany in the last ten years. The Federal Rehabilitation Council has given its members the opportunity of bringing their services and benefits into line. It has submitted suggestions for improvements of the legislative provisions to the legislative body. Special attention has been given, in this context, to the conclusion of the \"special agreements\" on behalf of the different rehabilitation agencies designed to ensure the speedy and smooth completion of the rehabilitation process. Furthermore, the Federal Rehabilitation Council has attended to the problems of the disabled and defended the interest of various disability groups. In addition, the Federal Rehabilitation Council has endeavoured to contribute to the international exchange of experience. Special attention has been placed on public education. As rehabilitation will not be allowed to stand still, the Federal Rehabilitation Council will base its future work on this principle.", "contents": "[The Federal Rehabilitation Council - a review of the last decade and prospects for the future (author's transl)]. Ten years have now passed since the foundation of the Federal Rehabilitation Council. Its work has both reflected and guided the development of rehabilitation in the Federal Republic of Germany in the last ten years. The Federal Rehabilitation Council has given its members the opportunity of bringing their services and benefits into line. It has submitted suggestions for improvements of the legislative provisions to the legislative body. Special attention has been given, in this context, to the conclusion of the \"special agreements\" on behalf of the different rehabilitation agencies designed to ensure the speedy and smooth completion of the rehabilitation process. Furthermore, the Federal Rehabilitation Council has attended to the problems of the disabled and defended the interest of various disability groups. In addition, the Federal Rehabilitation Council has endeavoured to contribute to the international exchange of experience. Special attention has been placed on public education. As rehabilitation will not be allowed to stand still, the Federal Rehabilitation Council will base its future work on this principle."}
{"id": "PMID:482729", "title": "[Clinical psychology in rehabilitative cardiology - a working model (author's transl)].", "content": "The article presents a model for the psychological treatment within the framework of an in-patient rehabilitation programme for patients suffering from heart and circulatory diseases. On the basis of research work investigating the psychological factors of heart diseases, this model describes details of a clinical-psychological intervention and psychotherapeutic/psychosomatic supervision tested over a two-year period at a specialised rehabilitation centre for heart and circulatory diseases.", "contents": "[Clinical psychology in rehabilitative cardiology - a working model (author's transl)]. The article presents a model for the psychological treatment within the framework of an in-patient rehabilitation programme for patients suffering from heart and circulatory diseases. On the basis of research work investigating the psychological factors of heart diseases, this model describes details of a clinical-psychological intervention and psychotherapeutic/psychosomatic supervision tested over a two-year period at a specialised rehabilitation centre for heart and circulatory diseases."}
{"id": "PMID:482730", "title": "[The social situation of spinal cord-injured persons -- an empirical study (author's transl)].", "content": "Using information from patients, over a period of almost ten years, who were hospitalised with spinal cord injuries at a specialised clinic, the Ludwigshafen Industrial Injuries Insurance Institute Accident Hospital -- Medical Director: W. Arens, M.D. -- this empirical study surveys the vocational and financial as well as the family and interpersonal situation of spinal cord-injured persons in the Federal Republic of Germany. The study was performed by means of mailed questionnaires of which 50% were returned. In addition to the composition of the sample with regard to the type of lesion and social status the study focused on sexual abilities and problems. The identified behavioural disturbances of the studied persons were related to counselling and counselling opportunities. he study was financed in part by the Arbeitsgemeinschaft der s\u00fcdwestdeutschen evangelischen Sozialfachhochschulen (working group of the southwest german protestant politechnics specialising in training for social careers) in Darmstadt, Fribourg/Brsg., Ludwigshafen and Reutlingen.", "contents": "[The social situation of spinal cord-injured persons -- an empirical study (author's transl)]. Using information from patients, over a period of almost ten years, who were hospitalised with spinal cord injuries at a specialised clinic, the Ludwigshafen Industrial Injuries Insurance Institute Accident Hospital -- Medical Director: W. Arens, M.D. -- this empirical study surveys the vocational and financial as well as the family and interpersonal situation of spinal cord-injured persons in the Federal Republic of Germany. The study was performed by means of mailed questionnaires of which 50% were returned. In addition to the composition of the sample with regard to the type of lesion and social status the study focused on sexual abilities and problems. The identified behavioural disturbances of the studied persons were related to counselling and counselling opportunities. he study was financed in part by the Arbeitsgemeinschaft der s\u00fcdwestdeutschen evangelischen Sozialfachhochschulen (working group of the southwest german protestant politechnics specialising in training for social careers) in Darmstadt, Fribourg/Brsg., Ludwigshafen and Reutlingen."}
{"id": "PMID:482731", "title": "[The work of local teachers of the deaf at danish audiological institutes (author's transl)].", "content": "In Denmark, the audio-paedagogical care of hearing-impaired adults is examplarily organised. A considerable role, within this rehabilitation system, is played by the local teachers of the deaf who, in co-operation with the national audiological institutes, do most of the audio-paedagogical work.", "contents": "[The work of local teachers of the deaf at danish audiological institutes (author's transl)]. In Denmark, the audio-paedagogical care of hearing-impaired adults is examplarily organised. A considerable role, within this rehabilitation system, is played by the local teachers of the deaf who, in co-operation with the national audiological institutes, do most of the audio-paedagogical work."}
{"id": "PMID:482733", "title": "The effect of an intrauterine device on mast cell numbers and distribution in the hamster uterus.", "content": "The effect of an IUD on mast cell numbers and distribution in the uterus was studied in castrated, castrated hormone-treated and cycling hamsters. The device had a stimulatory effect on total mast cell numbers in those animals that received no treatment, peanut oil, or estrogen therapy and in all cycling animals. The device also apparently causes mast cells to be redistributed in the different areas of the uterus. The results indicate that the IUD alters the uterine mast cell response to exogenous hormones and cycle times.", "contents": "The effect of an intrauterine device on mast cell numbers and distribution in the hamster uterus. The effect of an IUD on mast cell numbers and distribution in the uterus was studied in castrated, castrated hormone-treated and cycling hamsters. The device had a stimulatory effect on total mast cell numbers in those animals that received no treatment, peanut oil, or estrogen therapy and in all cycling animals. The device also apparently causes mast cells to be redistributed in the different areas of the uterus. The results indicate that the IUD alters the uterine mast cell response to exogenous hormones and cycle times."}
{"id": "PMID:482734", "title": "In vitro studies on resealed erythrocyte ghosts as protein carriers.", "content": "Resealed erythrocyte ghosts have been proposed as in vivo carriers for enzyme replacement therapy of inherited metabolic diseases. In comparative studies of methods for reversible hypotonic haemolysis of erythrocytes, a five-fold increased entrapment of human serum albumin was obtained by use of a dialysis procedure instead of direct dilution. The percentage incorporation of protein was also affected by varying mixing procedure, haematocrit, lysis, and resealing times but not by varying buffer composition or added protein concentrations over a wide range. Higher protein entrapment was observed with time-expired blood compared to fresh blood and this may be ascribed to increased osmotic fragility of the erythrocyte membrane in stored cells. Haemolysed and resealed ghosts prepared by any method used were smaller than normal erythrocytes as measured with a Coulter Counter and with a fluorescence-activated cell sorter, and protein entrapment reduced the ghost size further.", "contents": "In vitro studies on resealed erythrocyte ghosts as protein carriers. Resealed erythrocyte ghosts have been proposed as in vivo carriers for enzyme replacement therapy of inherited metabolic diseases. In comparative studies of methods for reversible hypotonic haemolysis of erythrocytes, a five-fold increased entrapment of human serum albumin was obtained by use of a dialysis procedure instead of direct dilution. The percentage incorporation of protein was also affected by varying mixing procedure, haematocrit, lysis, and resealing times but not by varying buffer composition or added protein concentrations over a wide range. Higher protein entrapment was observed with time-expired blood compared to fresh blood and this may be ascribed to increased osmotic fragility of the erythrocyte membrane in stored cells. Haemolysed and resealed ghosts prepared by any method used were smaller than normal erythrocytes as measured with a Coulter Counter and with a fluorescence-activated cell sorter, and protein entrapment reduced the ghost size further."}
{"id": "PMID:482735", "title": "The role of serotonin in Masugi nephritis in rabbits.", "content": "In 12 rabbits with induced experimental Masugi nephritis and in 6 control rabbits the level of 5-HT in whole blood and 5-HIAA excretion in urine/24 h were studied. In all animals of the experimental group a significant increase of 5-HT and 5-HIAA was found, which was strictly correlated in time with two-phase immunological reaction occurring in Masugi nephritis. Moreover, the electorn microscope examinations showed a distinct thickening of glomerular basement membrane on the 8th day of experiment, i.e., at the time of maximum 5-HT increase in the blood, which according to the literature, can be interpreted as having been caused, if not exclusively, so at least among others, by direct action of endogenous 5-HT.", "contents": "The role of serotonin in Masugi nephritis in rabbits. In 12 rabbits with induced experimental Masugi nephritis and in 6 control rabbits the level of 5-HT in whole blood and 5-HIAA excretion in urine/24 h were studied. In all animals of the experimental group a significant increase of 5-HT and 5-HIAA was found, which was strictly correlated in time with two-phase immunological reaction occurring in Masugi nephritis. Moreover, the electorn microscope examinations showed a distinct thickening of glomerular basement membrane on the 8th day of experiment, i.e., at the time of maximum 5-HT increase in the blood, which according to the literature, can be interpreted as having been caused, if not exclusively, so at least among others, by direct action of endogenous 5-HT."}
{"id": "PMID:482736", "title": "Evaluation of 133Xe clearance curves in the study of lingual blood flow in dogs.", "content": "Lingual blood flow was studied in dogs by the 133Xe clearance technique under control conditions, during epinephrine infusion in response to the combined treatment with Regitin and epinephrine. The composite washout curves recorded during control conditions could be resolved into three monoesponential components. It is suggested that the steep initial slope (component I)is caused by the fraction of blood that perfuses the A-V anastomoses of the tongue. Component II and III appear to the effect of epinephrine infusion might indicate the closure of arterio-venous anastomoses containing alpha receptors. Regitin pretreatment could prevent the closure of the arterio-venous anastomoses elicited by epinephrine infusion. The beta receptor stimulating activity of epinephrine migh account for the augmentation of blood flow to lingual musculature.", "contents": "Evaluation of 133Xe clearance curves in the study of lingual blood flow in dogs. Lingual blood flow was studied in dogs by the 133Xe clearance technique under control conditions, during epinephrine infusion in response to the combined treatment with Regitin and epinephrine. The composite washout curves recorded during control conditions could be resolved into three monoesponential components. It is suggested that the steep initial slope (component I)is caused by the fraction of blood that perfuses the A-V anastomoses of the tongue. Component II and III appear to the effect of epinephrine infusion might indicate the closure of arterio-venous anastomoses containing alpha receptors. Regitin pretreatment could prevent the closure of the arterio-venous anastomoses elicited by epinephrine infusion. The beta receptor stimulating activity of epinephrine migh account for the augmentation of blood flow to lingual musculature."}
{"id": "PMID:482737", "title": "Blood pressure and adipose tissue linoleic acid.", "content": "In a natural, \"free-living population\" of 650 men, surveyed with the purpose of health assessment within an epidemiological design, a strongly significant negative correlation between the relative linoleic acid composition of adipose tissue and blood pressures was found (P less than 0.001). This correlation remained significant when age and weight were statistically controlled for. Thus, dietary, lipid-lowering linoleic acid seems to effect blood pressures as well in a favorable way.", "contents": "Blood pressure and adipose tissue linoleic acid. In a natural, \"free-living population\" of 650 men, surveyed with the purpose of health assessment within an epidemiological design, a strongly significant negative correlation between the relative linoleic acid composition of adipose tissue and blood pressures was found (P less than 0.001). This correlation remained significant when age and weight were statistically controlled for. Thus, dietary, lipid-lowering linoleic acid seems to effect blood pressures as well in a favorable way."}
{"id": "PMID:482738", "title": "Superselective vagotomy sparing blood vessels in the rat. A useful tool in stress ulcer prevention.", "content": "In male Sprague-Dawley rats a microsurgical technique for highly selective vagotomy (HSV; syn. proximal gastric vagotomy) and for superselective vagotomy (SSV; cutting of proximal vagal fibers but sparing the blood vessels) has been developed. Basal acid secretion in both preparations was appr. 60% lower than in sham-operated controls. Acid response to 2-Deoxy-D-glucose (35 mg/kg/h over 4 h) was negative in HSV and SSV rats, whereas in sham rats acid output rose significantly. HSV, during mild stress (= control conditions), renders rats more susceptible to gastric stress ulcerations, whereas SSV with intact mucosal blood flow protects gastric mucosa almost completely. Although SSV, during severe restraint stress, cannot prevent the stress-induced breakdown of mucosal blood flow, gastric ulcerations are reduced to 40% of HSV and sham-operated control rats. SSV appears an elegant tool in stress ulcer research.", "contents": "Superselective vagotomy sparing blood vessels in the rat. A useful tool in stress ulcer prevention. In male Sprague-Dawley rats a microsurgical technique for highly selective vagotomy (HSV; syn. proximal gastric vagotomy) and for superselective vagotomy (SSV; cutting of proximal vagal fibers but sparing the blood vessels) has been developed. Basal acid secretion in both preparations was appr. 60% lower than in sham-operated controls. Acid response to 2-Deoxy-D-glucose (35 mg/kg/h over 4 h) was negative in HSV and SSV rats, whereas in sham rats acid output rose significantly. HSV, during mild stress (= control conditions), renders rats more susceptible to gastric stress ulcerations, whereas SSV with intact mucosal blood flow protects gastric mucosa almost completely. Although SSV, during severe restraint stress, cannot prevent the stress-induced breakdown of mucosal blood flow, gastric ulcerations are reduced to 40% of HSV and sham-operated control rats. SSV appears an elegant tool in stress ulcer research."}
{"id": "PMID:482758", "title": "[Crossed aphasia with jargon aphasia in two right-handed patients (author's transl)].", "content": "Two cases of crossed aphasia in right-handed patients are reported. The oral language of these two patients was characterized by a reduction in its lexical and grammatical content. Their written language, however, was a veritable jargon with anosognosia. The jargon aphasia of the first patient consisted mainly of neologisms. The written language of the second patient was slightly less disorganized and corresponded to an asemantic jargon. The existence of this jargon aphasia gives these two observations an exceptional character in relation to other cases of crossed aphasia in right-handed patients. It supplies new information which can be the basis for discussion on the relations between cerebral laterlization of language functions and manual preference.", "contents": "[Crossed aphasia with jargon aphasia in two right-handed patients (author's transl)]. Two cases of crossed aphasia in right-handed patients are reported. The oral language of these two patients was characterized by a reduction in its lexical and grammatical content. Their written language, however, was a veritable jargon with anosognosia. The jargon aphasia of the first patient consisted mainly of neologisms. The written language of the second patient was slightly less disorganized and corresponded to an asemantic jargon. The existence of this jargon aphasia gives these two observations an exceptional character in relation to other cases of crossed aphasia in right-handed patients. It supplies new information which can be the basis for discussion on the relations between cerebral laterlization of language functions and manual preference."}
{"id": "PMID:482759", "title": "[Granulomatous myositis during collagenosis (one case) and suprasellar dysgerminoma (one case) (author's transl)].", "content": "Two cases of mainly interstitial myositis with epitheloid and giant cells are reported. In the first case, the late amyotrophic-type paralysis of the two girdles, of the clinical myositis type, was associated with severe myocardial damage, the atrioventricular conduction disorder requiring a pace-maker. This observation would appear to be a true polymyositis of the collagen diseases, in view of the associated skin affection, death occurring within a year, a large increase in serum muscle enzymes and urine creatine, the existence of a rheumatoid factor and striated muscle antibodies, and a large increase in M and G immunoglobulins. In the 2nd case, the amyotrophic affection of the pelvic girdle was associated with progressive blindness, diabetes insipidus, and anterior pituitary insufficiency. Death occurred after two years and autopsy showed a suprasellar dysgerminoma (ectopic pinealoma) without any visceral localization of sarcoidosis. The authors discuss the concept of granulomatous polymyositis, autonomous with respect to the sarcoidosis, and sometimes symptomatic of an inflammatory connective tissue condition or a malignant tumor.", "contents": "[Granulomatous myositis during collagenosis (one case) and suprasellar dysgerminoma (one case) (author's transl)]. Two cases of mainly interstitial myositis with epitheloid and giant cells are reported. In the first case, the late amyotrophic-type paralysis of the two girdles, of the clinical myositis type, was associated with severe myocardial damage, the atrioventricular conduction disorder requiring a pace-maker. This observation would appear to be a true polymyositis of the collagen diseases, in view of the associated skin affection, death occurring within a year, a large increase in serum muscle enzymes and urine creatine, the existence of a rheumatoid factor and striated muscle antibodies, and a large increase in M and G immunoglobulins. In the 2nd case, the amyotrophic affection of the pelvic girdle was associated with progressive blindness, diabetes insipidus, and anterior pituitary insufficiency. Death occurred after two years and autopsy showed a suprasellar dysgerminoma (ectopic pinealoma) without any visceral localization of sarcoidosis. The authors discuss the concept of granulomatous polymyositis, autonomous with respect to the sarcoidosis, and sometimes symptomatic of an inflammatory connective tissue condition or a malignant tumor."}
{"id": "PMID:482760", "title": "[Bilateral internuclear ophthalmoplegia (author's transl)].", "content": "A case is reported of a patient with bilateral internuclear ophtalmoplegia which followed a single limited lesion of the two medial longitudinal fasciculi (M.L.F.), with degeneration of the right internal nucleus. The authors feel that the exact localization of this minute lesions, associated with right internal nucleus degeneration, can assist in supplying replies to some unanswered questions concerning the M.L.F. syndrome.", "contents": "[Bilateral internuclear ophthalmoplegia (author's transl)]. A case is reported of a patient with bilateral internuclear ophtalmoplegia which followed a single limited lesion of the two medial longitudinal fasciculi (M.L.F.), with degeneration of the right internal nucleus. The authors feel that the exact localization of this minute lesions, associated with right internal nucleus degeneration, can assist in supplying replies to some unanswered questions concerning the M.L.F. syndrome."}
{"id": "PMID:482761", "title": "[Value of computer assisted tomography in the exploration of the sellar and supra sellar structures (author's transl)].", "content": "The results obtained in 145 cases of space-occupying lesions in the sellar and suprasellar structures, after exploration with computer assisted tomography, were used to assess the technical and diagnostic value of this procedure. Precise details of the localization and extent of the lesion can be obtained, but not the nature of the lesion in many cases.", "contents": "[Value of computer assisted tomography in the exploration of the sellar and supra sellar structures (author's transl)]. The results obtained in 145 cases of space-occupying lesions in the sellar and suprasellar structures, after exploration with computer assisted tomography, were used to assess the technical and diagnostic value of this procedure. Precise details of the localization and extent of the lesion can be obtained, but not the nature of the lesion in many cases."}
{"id": "PMID:482762", "title": "[Monocular circumduction nystagmus (author's transl)].", "content": "Monocular circumduction nystagmus is a very rare ocular movement. A type of circumduction movement, it is caused by successive movements of all the ocular muscles within the framework of a pendular nystagmus. As a monocular movement, it raises the question of the physiopathological mechanism of such a movement which is so complex and is also limited to one eye. The existence of a monocular form of circumduction nystagmus makes it necessary to revise the mechanism of pendular nystagmus.", "contents": "[Monocular circumduction nystagmus (author's transl)]. Monocular circumduction nystagmus is a very rare ocular movement. A type of circumduction movement, it is caused by successive movements of all the ocular muscles within the framework of a pendular nystagmus. As a monocular movement, it raises the question of the physiopathological mechanism of such a movement which is so complex and is also limited to one eye. The existence of a monocular form of circumduction nystagmus makes it necessary to revise the mechanism of pendular nystagmus."}
{"id": "PMID:482784", "title": "[CT scanning of the orbita (author's transl)].", "content": "Basing on selected cases, the efficiency spectrum of modern CT apparatus in the diagnosis of orbital disease is described. Besides exact localisation of retrobulbar space-occupying growths, CT scanning enables good identification of pathological processes at the bulbus and those of parabulbar location, at the lacrimal gland, the optic nerve and the ocular muscles. Extraorbital changes are clearly identifiable.", "contents": "[CT scanning of the orbita (author's transl)]. Basing on selected cases, the efficiency spectrum of modern CT apparatus in the diagnosis of orbital disease is described. Besides exact localisation of retrobulbar space-occupying growths, CT scanning enables good identification of pathological processes at the bulbus and those of parabulbar location, at the lacrimal gland, the optic nerve and the ocular muscles. Extraorbital changes are clearly identifiable."}
{"id": "PMID:482785", "title": "[The X-ray film of the petrosal bone in the special projection according to Chauss\u00e9e III (author's transl)].", "content": "For the purpose of a better appreciation of the x-ray film of the petrosal bone in the special projection according to Chauss\u00e9e III, the roentgenogram was divided into fifteen individual subsequent pictures and explained step by step. Together with the standard x-ray films, this particular roentgenogram provides a spatial idea of the tympanic cavity and enables accurate assessment of the destruction caused by chronic epitympanal otitis media as well as cholesteatoma. In many cases this eliminates the necessity of performing tomography of the middle ear.", "contents": "[The X-ray film of the petrosal bone in the special projection according to Chauss\u00e9e III (author's transl)]. For the purpose of a better appreciation of the x-ray film of the petrosal bone in the special projection according to Chauss\u00e9e III, the roentgenogram was divided into fifteen individual subsequent pictures and explained step by step. Together with the standard x-ray films, this particular roentgenogram provides a spatial idea of the tympanic cavity and enables accurate assessment of the destruction caused by chronic epitympanal otitis media as well as cholesteatoma. In many cases this eliminates the necessity of performing tomography of the middle ear."}
{"id": "PMID:482786", "title": "[Efficiency of radiological puncture cytology from the viewpoint of the pathologist (author's transl)].", "content": "Optimal puncture and smear technique, close communication between the radiologist and cytologist, and great experience of the cytologist in the assessment of the preparations, are an absolute \"must\" the ensure high accuracy of x-ray guided fine needle biopsy.", "contents": "[Efficiency of radiological puncture cytology from the viewpoint of the pathologist (author's transl)]. Optimal puncture and smear technique, close communication between the radiologist and cytologist, and great experience of the cytologist in the assessment of the preparations, are an absolute \"must\" the ensure high accuracy of x-ray guided fine needle biopsy."}
{"id": "PMID:482787", "title": "[Technique and use of fine needle puncture for thyroid gland (author's transl)].", "content": "Fine needle puncture of cold and warm thyroid gland nodes has its definite place in the diagnostic repertory of thyroid diseases. This method can be performed rapidly and easily. It has a low complication rate and imparts to the physician a maximum of information in association with in-vitro tests and scintigram. Indication and technique of fine needle puncture are described and discussed.", "contents": "[Technique and use of fine needle puncture for thyroid gland (author's transl)]. Fine needle puncture of cold and warm thyroid gland nodes has its definite place in the diagnostic repertory of thyroid diseases. This method can be performed rapidly and easily. It has a low complication rate and imparts to the physician a maximum of information in association with in-vitro tests and scintigram. Indication and technique of fine needle puncture are described and discussed."}
{"id": "PMID:482788", "title": "[X-ray controlled percutaneous needle aspiration biopsy of the lungs (author's transl)].", "content": "Between 1976 and 1978 percutaneous needle aspiration biopsies of 120 pulmonary and pleural lesions were performed. Cytologic examination of malignant and benign lesions was correct in 64% of the cases, false negative results were obtained in 19%. Complications included: pneumothorax in 21 patients (11 requiring chest tube placement); insignificant hemothorax in 5 and hemoptysis in 1. Needle biopsy of intrathoracic lesions proved to be technically simple and relatively safe. This procedure allows early diagnosis of malignant lung tumours which may improve long term prognosis.", "contents": "[X-ray controlled percutaneous needle aspiration biopsy of the lungs (author's transl)]. Between 1976 and 1978 percutaneous needle aspiration biopsies of 120 pulmonary and pleural lesions were performed. Cytologic examination of malignant and benign lesions was correct in 64% of the cases, false negative results were obtained in 19%. Complications included: pneumothorax in 21 patients (11 requiring chest tube placement); insignificant hemothorax in 5 and hemoptysis in 1. Needle biopsy of intrathoracic lesions proved to be technically simple and relatively safe. This procedure allows early diagnosis of malignant lung tumours which may improve long term prognosis."}
{"id": "PMID:482789", "title": "[Technique and results of percutaneous transthoracal fine-needle biopsy of lung lesions (author's transl)].", "content": "Find needle biopsy of tumours of the lung and mediastinum represents an accurate method which can be performed pain-free and rapidly without undue effort and cost. It can be repeated at any desired time. Absolute contraindications are very rare. This method can be applied also in outpatients, provided certain precautions are observed. In many cases, it eliminates the far greater risk of exploratory thoracotomy. Both peripheral and central lung tumours, including mediastinal tumours, can be identified and differentiated. More than nine out of ten bronchial carcinomas are clearly identified. In 60--70% of all punctures, the pathologist is successful in accurately establishing the type of cell involved. As far as the patient himself is concerned, the method hardly involves any pain and does not cause much discomfort. A frequent complication is the usually asymptomatic pneumothorax, which occurs in less than 30% of all punctures. In a few rare cases, suction drainage will be necessary. Generally speaking, and in all other respects, fine needle biopsy is a low-risk procedure.", "contents": "[Technique and results of percutaneous transthoracal fine-needle biopsy of lung lesions (author's transl)]. Find needle biopsy of tumours of the lung and mediastinum represents an accurate method which can be performed pain-free and rapidly without undue effort and cost. It can be repeated at any desired time. Absolute contraindications are very rare. This method can be applied also in outpatients, provided certain precautions are observed. In many cases, it eliminates the far greater risk of exploratory thoracotomy. Both peripheral and central lung tumours, including mediastinal tumours, can be identified and differentiated. More than nine out of ten bronchial carcinomas are clearly identified. In 60--70% of all punctures, the pathologist is successful in accurately establishing the type of cell involved. As far as the patient himself is concerned, the method hardly involves any pain and does not cause much discomfort. A frequent complication is the usually asymptomatic pneumothorax, which occurs in less than 30% of all punctures. In a few rare cases, suction drainage will be necessary. Generally speaking, and in all other respects, fine needle biopsy is a low-risk procedure."}
{"id": "PMID:482790", "title": "[Technique and diagnostic value of percutaneous transhepatic cholangiography (author's transl)].", "content": "Percutaneous transhepatic cholangiographic (PTC) serves to perform antegrade bile duct visualisation in obstructive jaundice. It gives exact information on the extent of the flow obstruction and the permeability of the prestenotic area. In most cases, it is possible to differentiate between stone, inflammation and malignant change, but it as a rule most difficult to diagnose the type of tumour, if any. Basing on the authors' own experience with 152 PTC, technique, indications, contraindications and complications as well as differential diagnostic possibilities are described. The complication rate has dropped very clearly since the Chiba needle was introduced.", "contents": "[Technique and diagnostic value of percutaneous transhepatic cholangiography (author's transl)]. Percutaneous transhepatic cholangiographic (PTC) serves to perform antegrade bile duct visualisation in obstructive jaundice. It gives exact information on the extent of the flow obstruction and the permeability of the prestenotic area. In most cases, it is possible to differentiate between stone, inflammation and malignant change, but it as a rule most difficult to diagnose the type of tumour, if any. Basing on the authors' own experience with 152 PTC, technique, indications, contraindications and complications as well as differential diagnostic possibilities are described. The complication rate has dropped very clearly since the Chiba needle was introduced."}
{"id": "PMID:482791", "title": "[Technique of percutaneous transhepatic drainage of the bile ducts and its spectrum of application (author's transl)].", "content": "Percutaneous transhepatic drainage (PTD) -- internal or external -- is an important method in the treatment of obstructive jaundice. Its value rests in the temporary relief of obstructions of the bile ducts before surgery, or in the palliative treatment of patients with inoperable tumours. The article describes the technique, indications, complications and difficulties. The authors' own experience is based on 19 successful percutaneous transhepatic drainages. The method is easy to apprehend and has a low complication rate.", "contents": "[Technique of percutaneous transhepatic drainage of the bile ducts and its spectrum of application (author's transl)]. Percutaneous transhepatic drainage (PTD) -- internal or external -- is an important method in the treatment of obstructive jaundice. Its value rests in the temporary relief of obstructions of the bile ducts before surgery, or in the palliative treatment of patients with inoperable tumours. The article describes the technique, indications, complications and difficulties. The authors' own experience is based on 19 successful percutaneous transhepatic drainages. The method is easy to apprehend and has a low complication rate."}
{"id": "PMID:482792", "title": "[Indication and diagnostic value of roentgen-guided fine-needle puncture of space-occupying renal lesions (author's transl)].", "content": "Roentgen-guided fine needle puncture of space-occupying renal lesions as an important additive radiologic procedure is able to solve differential diagnostic problems of liquid or solid renal lesions in a quick, accurate and safey way. It rules out a solid neoplasm, provides the surgeon with a maximum of preoperative information and helps to avoid operations on aged and other poor-risk patients. After description of the puncture technique we report the result of 217 fine needle punctures of the kidney over 13 years.", "contents": "[Indication and diagnostic value of roentgen-guided fine-needle puncture of space-occupying renal lesions (author's transl)]. Roentgen-guided fine needle puncture of space-occupying renal lesions as an important additive radiologic procedure is able to solve differential diagnostic problems of liquid or solid renal lesions in a quick, accurate and safey way. It rules out a solid neoplasm, provides the surgeon with a maximum of preoperative information and helps to avoid operations on aged and other poor-risk patients. After description of the puncture technique we report the result of 217 fine needle punctures of the kidney over 13 years."}
{"id": "PMID:482793", "title": "[Percutaneous translumbar pyelography -- a method for the diagnostic clarification of obstructive uropathy (author's transl)].", "content": "Percutaneous translumbar pyelography is indicated when excretory has failed to demonstrate the cause or site of obstructive uropathy and retrograde pyelography is impossible, undesirable or contraindicated. Under prophylactic treatment with antibiotics translumbar antegrade pyelography is safe, simple and accurate and in selected cases the only diagnostic method to establish exact diagnosis without which appropriate therapy cannot be planned. The method is described and the results of 30 percutaneous translumbar pyelographies obtained from 2603 angiographies over 12 years are reported.", "contents": "[Percutaneous translumbar pyelography -- a method for the diagnostic clarification of obstructive uropathy (author's transl)]. Percutaneous translumbar pyelography is indicated when excretory has failed to demonstrate the cause or site of obstructive uropathy and retrograde pyelography is impossible, undesirable or contraindicated. Under prophylactic treatment with antibiotics translumbar antegrade pyelography is safe, simple and accurate and in selected cases the only diagnostic method to establish exact diagnosis without which appropriate therapy cannot be planned. The method is described and the results of 30 percutaneous translumbar pyelographies obtained from 2603 angiographies over 12 years are reported."}
{"id": "PMID:482841", "title": "[Phalangeal osteoid osteoma (13 cases)].", "content": "Thirteen cases of osteoid osteoma of the phalanxes are reported: 10 fingers and 3 toes. Any phalanx may be affected, with no preference for a particular site, in the young adult. Segmentary tumefaction of the soft parts near the lesion is a more consistent sign than pain, which is often quite minimal. Radiographies demonstrate the increase in the size of the phalanx, whose matrix is condensed. All radiographic techniques must be used to determine the exact location of the nidus, that is always very small (1 to 2 mm), and whose total removal is the only guarantee of cure.", "contents": "[Phalangeal osteoid osteoma (13 cases)]. Thirteen cases of osteoid osteoma of the phalanxes are reported: 10 fingers and 3 toes. Any phalanx may be affected, with no preference for a particular site, in the young adult. Segmentary tumefaction of the soft parts near the lesion is a more consistent sign than pain, which is often quite minimal. Radiographies demonstrate the increase in the size of the phalanx, whose matrix is condensed. All radiographic techniques must be used to determine the exact location of the nidus, that is always very small (1 to 2 mm), and whose total removal is the only guarantee of cure."}
{"id": "PMID:482842", "title": "[Biosynthesis of PGE2 and PGF2 alpha in normal and pathological human synovial fluid].", "content": "The biosynthesis capacity of PGE2 and PGF2 alpha in normal and pathological (rheumatoid arthritis and osteo-arthrosis) synovialis was studied using the radio-immunological method, simultaneously on homogenized tissues and the microsome fraction, with or without addition of exogenous arachidonic acid. Among the results obtained, the most significant concern rheumatoid polyarthritis: a net increase in PGF2 alpha and especially in PGE2, more pronounced in the homogenized emulsion than in the microsomial fraction. Exogenous arachidonic acid increases the biosynthesis capacity of the PG. The prospective interest of this model is underlined: the possible introduction in the incubation medium of various drugs, making it possible to study the various factors taking part in the biosynthesis of PG (phospholipase A2 and prostaglandin-synthetase).", "contents": "[Biosynthesis of PGE2 and PGF2 alpha in normal and pathological human synovial fluid]. The biosynthesis capacity of PGE2 and PGF2 alpha in normal and pathological (rheumatoid arthritis and osteo-arthrosis) synovialis was studied using the radio-immunological method, simultaneously on homogenized tissues and the microsome fraction, with or without addition of exogenous arachidonic acid. Among the results obtained, the most significant concern rheumatoid polyarthritis: a net increase in PGF2 alpha and especially in PGE2, more pronounced in the homogenized emulsion than in the microsomial fraction. Exogenous arachidonic acid increases the biosynthesis capacity of the PG. The prospective interest of this model is underlined: the possible introduction in the incubation medium of various drugs, making it possible to study the various factors taking part in the biosynthesis of PG (phospholipase A2 and prostaglandin-synthetase)."}
{"id": "PMID:482843", "title": "[Study of cellular elements in synovial fluid in scanning electron microscopy].", "content": "Scanning electron microscopy allows a tridimensional morphological study of the cellular elements of the pathological human synovial fluid. The cells are grouped into two populations: macrophage cells and round cells, whose differentiation is more difficult: polynuclears, lymphocytes and synovial cells. The combination of scanning electron microscopy and X-ray diffraction makes it possible to describe and indentify crystals of sodium urate and of triclinical, dehydrated calcium pyrophosphate.", "contents": "[Study of cellular elements in synovial fluid in scanning electron microscopy]. Scanning electron microscopy allows a tridimensional morphological study of the cellular elements of the pathological human synovial fluid. The cells are grouped into two populations: macrophage cells and round cells, whose differentiation is more difficult: polynuclears, lymphocytes and synovial cells. The combination of scanning electron microscopy and X-ray diffraction makes it possible to describe and indentify crystals of sodium urate and of triclinical, dehydrated calcium pyrophosphate."}
{"id": "PMID:482844", "title": "[Value of metrizamide myelography with tomodensitometry for study of the cervical spine].", "content": "52 metrizamide myelographies practiced with tomodensitometry were studies (23 normal cases); 6 cervicarthrosic myelopathies; 2 intra medullar tumors; 1 extra medullar tumor; 19 syringomyelias). The technical procedures are discussed for the different diseases. This test has proven highly interesting for the study of syringomyelias and tumors. It allows the presence of narrow canals to be demonstrated, but does not yet indicate discal hernias. The role of tomodensitometry, in relation to other exams, is demonstrated.", "contents": "[Value of metrizamide myelography with tomodensitometry for study of the cervical spine]. 52 metrizamide myelographies practiced with tomodensitometry were studies (23 normal cases); 6 cervicarthrosic myelopathies; 2 intra medullar tumors; 1 extra medullar tumor; 19 syringomyelias). The technical procedures are discussed for the different diseases. This test has proven highly interesting for the study of syringomyelias and tumors. It allows the presence of narrow canals to be demonstrated, but does not yet indicate discal hernias. The role of tomodensitometry, in relation to other exams, is demonstrated."}
{"id": "PMID:482846", "title": "[Magnesium and several metabolic osteopathies].", "content": "Magnesium is a constituant of the bone minerals. Changes in its intra-bone distribution have repercussions on the distribution of calcium and on the hormonal regulators of calcium. The importance of magnesium in bone pathology is far from being completely elucidated. Certain factors are now commonly admitted, for example the inhibition of the parathyroid secretion during magnesium deficiency. Others merit more complete and thorough study, it being highly unlikely that the bone is unaffected by changes in the organic magnesium content, given the multiple roles that magnesium plays in the bone. In this report, the authors provide well-known data on the physiology of magnesium, and discuss some of the relationships between magnesium disturbances and certain osteopathies.", "contents": "[Magnesium and several metabolic osteopathies]. Magnesium is a constituant of the bone minerals. Changes in its intra-bone distribution have repercussions on the distribution of calcium and on the hormonal regulators of calcium. The importance of magnesium in bone pathology is far from being completely elucidated. Certain factors are now commonly admitted, for example the inhibition of the parathyroid secretion during magnesium deficiency. Others merit more complete and thorough study, it being highly unlikely that the bone is unaffected by changes in the organic magnesium content, given the multiple roles that magnesium plays in the bone. In this report, the authors provide well-known data on the physiology of magnesium, and discuss some of the relationships between magnesium disturbances and certain osteopathies."}
{"id": "PMID:482851", "title": "Tissue eosinophils in ulcerative colitis.", "content": "A morphometric method has been used to quantitate eosinophils in the rectal lamina propria. The results from examination of paired biopsy specimens indicate that eosinophil distribution is uniform in the rectum. The number of eosinophils in specimens from patients with long-standing ulcerative colitis is significantly greater than that found in control subjects and increases with increasing clinical activity of the disease. In contrast, when biopsies are obtained from patients in a severe first attack of ulcerative colitis, eosinophil counts are not higher than in controls. The possible significance of these findings is discussed.", "contents": "Tissue eosinophils in ulcerative colitis. A morphometric method has been used to quantitate eosinophils in the rectal lamina propria. The results from examination of paired biopsy specimens indicate that eosinophil distribution is uniform in the rectum. The number of eosinophils in specimens from patients with long-standing ulcerative colitis is significantly greater than that found in control subjects and increases with increasing clinical activity of the disease. In contrast, when biopsies are obtained from patients in a severe first attack of ulcerative colitis, eosinophil counts are not higher than in controls. The possible significance of these findings is discussed."}
{"id": "PMID:482852", "title": "Exocrine pancreatic function after total gastrectomy.", "content": "The secretion of bicarbonate, lipase, and chymotrypsin into the duodenum in response to exogenous stimulation with secretin, 1 CU/kg-h, plus caerulein, 100 ng/kg-h, was investigated in 12 patients, on an average, 20.7 months after total gastrectomy and in 14 control subjects. The secretion of bicarbonate and lipase was significantly lower in patients than in controls. The reduction in outputs compared with the control values was 47.9%, 38.7%, and 24.2% respectively for bicarbonate, lipase, and chymotrypsin. Eight of the 12 patients (67%) had steatorrhoea. No significant correlation was found between this parameter and lipase output. It is concluded that the exocrine pancreatic function is impaired in the majority of patients subjected to total gastrectomy. The impairment, which particularly affects bicarbonate and lipase, is generally mild to moderate.", "contents": "Exocrine pancreatic function after total gastrectomy. The secretion of bicarbonate, lipase, and chymotrypsin into the duodenum in response to exogenous stimulation with secretin, 1 CU/kg-h, plus caerulein, 100 ng/kg-h, was investigated in 12 patients, on an average, 20.7 months after total gastrectomy and in 14 control subjects. The secretion of bicarbonate and lipase was significantly lower in patients than in controls. The reduction in outputs compared with the control values was 47.9%, 38.7%, and 24.2% respectively for bicarbonate, lipase, and chymotrypsin. Eight of the 12 patients (67%) had steatorrhoea. No significant correlation was found between this parameter and lipase output. It is concluded that the exocrine pancreatic function is impaired in the majority of patients subjected to total gastrectomy. The impairment, which particularly affects bicarbonate and lipase, is generally mild to moderate."}
{"id": "PMID:482854", "title": "ERCP in a small hospital.", "content": "A 1-year material of 75 ERCP investigations is reported from a small community hospital serving an area of 40,000 inhabitants. A cannulation success rate of 90.7% was found. No complications were seen. The use of the procedure in a small hospital is discussed.", "contents": "ERCP in a small hospital. A 1-year material of 75 ERCP investigations is reported from a small community hospital serving an area of 40,000 inhabitants. A cannulation success rate of 90.7% was found. No complications were seen. The use of the procedure in a small hospital is discussed."}
{"id": "PMID:482855", "title": "Parietal cell structure and acid secretion in the vagally innervated stomach and the vagally denervated fundic pouch in cats.", "content": "In seven cats provided with gastric fistula (GF) of the main stomach and a denervated Heidenhain pouch (HP) the structure of the innervated and denervated mucosa was examined by light and electron microscopy. In four of these cats dose-response curves for acid output to pentagastrin were determined before and after vagal denervation of the fundic pouch. Vagal denervation reduced the acid response to pentagastrin by decreasing both the secretory capacity and the sensitivity of the oxyntic glands. Accordingly, in the same cat the parietal cells of the HP were less sensitive to pentagastrin than the cells of the innervated main stomach. Light and electron microscopic investigations revealed that the fundic mucosa was thinner, and the size and proportion of the parietal cells was smaller in the HP than in the GF. It is likely that the morphological changes in the pouch were caused by the vagal denervation rather than by the loss of mucosal contact with food. The secretory and morphological changes produced by the vagotomy are not necessarily related.", "contents": "Parietal cell structure and acid secretion in the vagally innervated stomach and the vagally denervated fundic pouch in cats. In seven cats provided with gastric fistula (GF) of the main stomach and a denervated Heidenhain pouch (HP) the structure of the innervated and denervated mucosa was examined by light and electron microscopy. In four of these cats dose-response curves for acid output to pentagastrin were determined before and after vagal denervation of the fundic pouch. Vagal denervation reduced the acid response to pentagastrin by decreasing both the secretory capacity and the sensitivity of the oxyntic glands. Accordingly, in the same cat the parietal cells of the HP were less sensitive to pentagastrin than the cells of the innervated main stomach. Light and electron microscopic investigations revealed that the fundic mucosa was thinner, and the size and proportion of the parietal cells was smaller in the HP than in the GF. It is likely that the morphological changes in the pouch were caused by the vagal denervation rather than by the loss of mucosal contact with food. The secretory and morphological changes produced by the vagotomy are not necessarily related."}
{"id": "PMID:482856", "title": "The physiologic basis for clearance measurements in hepatology.", "content": "Three hepatic clearance regimes (flow-limited, general, and enzyme-limited) can be defined from a model of hepatic perfusion-elimination relationships. Substances that can be used for clearance measurements can thus be classified into three categories according to the relation between their kinetic elimination constants (Vmax, Km) and hepatic blood flow. The pathophysiologic and clinical importance of the clearance regimes is discussed with special emphasis on the effect of changes in hepatic blood flow and liver function. The criteria for choosing test substances within each regime are stated. This choice depends on the object of study for a clearance measurement (blood flow, drug elimination, liver function). Only the enzyme-limited clearance regime is suited for direct assessment of quantitative liver function ('true clearance'), while the other regimes depend more or less on blood flow.", "contents": "The physiologic basis for clearance measurements in hepatology. Three hepatic clearance regimes (flow-limited, general, and enzyme-limited) can be defined from a model of hepatic perfusion-elimination relationships. Substances that can be used for clearance measurements can thus be classified into three categories according to the relation between their kinetic elimination constants (Vmax, Km) and hepatic blood flow. The pathophysiologic and clinical importance of the clearance regimes is discussed with special emphasis on the effect of changes in hepatic blood flow and liver function. The criteria for choosing test substances within each regime are stated. This choice depends on the object of study for a clearance measurement (blood flow, drug elimination, liver function). Only the enzyme-limited clearance regime is suited for direct assessment of quantitative liver function ('true clearance'), while the other regimes depend more or less on blood flow."}
{"id": "PMID:482857", "title": "A simplified means of measuring the ventilatory response to carbon dioxide (STCO2) compared with the subjective and circulatory response to intrajejunal hypertonic glucose in the prediction of clinical dumpting.", "content": "Three methods previously suggested for the prediction of the dumping syndrome in patients undergoing gastric surgery were compared. Neither the subjective response nor the change in forearm muscle blood flow accompanying a preoperative intrajejunal test meal was found to separate potential 'clinical dumpers' from 'non-dumpers'. The ventilatory response to a rising alveolar carbon dioxide partial pressure, estimated by a simplified rebreathing technique, effectively identified those with a predisposition to dumping within the sample. Of the conventional measures of ventilatory response to carbon dioxide, the standardized threshold (STCO2) was found to be the most reliable.", "contents": "A simplified means of measuring the ventilatory response to carbon dioxide (STCO2) compared with the subjective and circulatory response to intrajejunal hypertonic glucose in the prediction of clinical dumpting. Three methods previously suggested for the prediction of the dumping syndrome in patients undergoing gastric surgery were compared. Neither the subjective response nor the change in forearm muscle blood flow accompanying a preoperative intrajejunal test meal was found to separate potential 'clinical dumpers' from 'non-dumpers'. The ventilatory response to a rising alveolar carbon dioxide partial pressure, estimated by a simplified rebreathing technique, effectively identified those with a predisposition to dumping within the sample. Of the conventional measures of ventilatory response to carbon dioxide, the standardized threshold (STCO2) was found to be the most reliable."}
{"id": "PMID:482858", "title": "Psychological, social, and surgical factors which influence success or failure after gastric operations.", "content": "The degree of a patient's emotional maturity and the supportive value of his home, working, and recreational environments were scored by psychosociological methods preoperatively in a group of 47 patients who were intensively investigated with regard to the dumping syndrome. After surgery, the result of the operation was independently assessed as a success or a failure. At that time, a careful review was performed of each patient's clinical state, with particular reference to postgastrectomy syndromes. Positive associations were found between surgical failure and emotional instability, recorded by an Eysenck personality inventory, and scored social deprivation. Failure also correlated with the sum of the postgastrectomy syndromes and with recurrent pain, heartburn, episodic diarrhoea, and psychiatric illness in particular. The dumping syndrome itself did not contribute to failure.", "contents": "Psychological, social, and surgical factors which influence success or failure after gastric operations. The degree of a patient's emotional maturity and the supportive value of his home, working, and recreational environments were scored by psychosociological methods preoperatively in a group of 47 patients who were intensively investigated with regard to the dumping syndrome. After surgery, the result of the operation was independently assessed as a success or a failure. At that time, a careful review was performed of each patient's clinical state, with particular reference to postgastrectomy syndromes. Positive associations were found between surgical failure and emotional instability, recorded by an Eysenck personality inventory, and scored social deprivation. Failure also correlated with the sum of the postgastrectomy syndromes and with recurrent pain, heartburn, episodic diarrhoea, and psychiatric illness in particular. The dumping syndrome itself did not contribute to failure."}
{"id": "PMID:482859", "title": "The role of altered gastric emptying in the initiation of clinical dumping.", "content": "The disposition to dumping was measured in 42 patients by finding their STCO2 before gastric surgery. Twenty of these had a low STCO2 and were therefore predisposed to experience dumping symptoms. Only 10 of these patients actually experienced clinical dumping when reviewed 1 year after surgery. At that time gastric emptying studies showed that those with clinical dumping had rapid gastric emptying in combination with a previously measured low STCO2, in contrast to those who did not experience dumping symptoms.", "contents": "The role of altered gastric emptying in the initiation of clinical dumping. The disposition to dumping was measured in 42 patients by finding their STCO2 before gastric surgery. Twenty of these had a low STCO2 and were therefore predisposed to experience dumping symptoms. Only 10 of these patients actually experienced clinical dumping when reviewed 1 year after surgery. At that time gastric emptying studies showed that those with clinical dumping had rapid gastric emptying in combination with a previously measured low STCO2, in contrast to those who did not experience dumping symptoms."}
{"id": "PMID:482860", "title": "Urolithiasis and hyperoxaluria in chronic inflammatory bowel disease.", "content": "The incidence of urolithiasis was registered in 87 patients with chronic inflammatory bowel disease and compared with that of renal oxalate excretion. All patients were studied while on a standardized diet with fixed amounts of fat, calcium, and oxalate. Pyelography had been performed in all. Nine, or 35%, of 26 hyperoxaluric patients had urolithiasis, compared with 14, or 23%, of 61 patients were normal renal oxalate excretion, the difference being statistically insignificant. No significant difference in urinary oxalate or urinary calcium in stone-formers as compared with non-stone-formers could be demonstrated. Oxalate was a more frequent component of calculi in patients with normal renal oxalate excretion than in patients with hyperoxalura. Thus, we were unable to demonstrate an increased incidence of urolithiasis in patients with hyperoxaluria compared with a control group with normal renal oxalate excretion. Our results cast doubt on the concept that enteric hyperoxaluria per se is the cause of stone diathesis in chronic inflammatory bowel disease.", "contents": "Urolithiasis and hyperoxaluria in chronic inflammatory bowel disease. The incidence of urolithiasis was registered in 87 patients with chronic inflammatory bowel disease and compared with that of renal oxalate excretion. All patients were studied while on a standardized diet with fixed amounts of fat, calcium, and oxalate. Pyelography had been performed in all. Nine, or 35%, of 26 hyperoxaluric patients had urolithiasis, compared with 14, or 23%, of 61 patients were normal renal oxalate excretion, the difference being statistically insignificant. No significant difference in urinary oxalate or urinary calcium in stone-formers as compared with non-stone-formers could be demonstrated. Oxalate was a more frequent component of calculi in patients with normal renal oxalate excretion than in patients with hyperoxalura. Thus, we were unable to demonstrate an increased incidence of urolithiasis in patients with hyperoxaluria compared with a control group with normal renal oxalate excretion. Our results cast doubt on the concept that enteric hyperoxaluria per se is the cause of stone diathesis in chronic inflammatory bowel disease."}
{"id": "PMID:482861", "title": "Clinical and pharmacological effectiveness of cimetidine in duodenal ulcer patients.", "content": "The plasma concentration of cimetidine was measured in 40 patients with duodenal ulcer after an oral dose of 200 mg cimetidine. The peak plasma concentration was on average 1.33 mg x 1(-1) (S.D. = 0.53) and the area under the plasma concentration curve (AUC) between 60 and 120 min after cimetidine was 1.13 mg x h x 1(-1) (S.D. = 0.48). The percentage inhibition (I%) of maximal acid output (MAO) to pentagastrin during this 60-min period was 49% (S.D. = 19) with a very low, but statistically significant, correlation with the AUC, r = 0.35 (p less than 0.05), thus demonstrating a very great individual variation in sensitivity to cimetidine. In 37 of the patients the time from start of treatment with cimetidine, 1.0 g/day, to disappearance of ulcer symptoms could be assessed, and it was found that neither the individual sensitivity to cimetidine, I%/AUC, nor the gastric secretory capacity, MAO, correlated significantly with the clinical effectiveness of the cimetidine treatment, although 8 patients with a low sensitivity to cimetidine and a high MAO improved more slowly (28 days) than the other 29 patients (13 days) (p less than 0.1).", "contents": "Clinical and pharmacological effectiveness of cimetidine in duodenal ulcer patients. The plasma concentration of cimetidine was measured in 40 patients with duodenal ulcer after an oral dose of 200 mg cimetidine. The peak plasma concentration was on average 1.33 mg x 1(-1) (S.D. = 0.53) and the area under the plasma concentration curve (AUC) between 60 and 120 min after cimetidine was 1.13 mg x h x 1(-1) (S.D. = 0.48). The percentage inhibition (I%) of maximal acid output (MAO) to pentagastrin during this 60-min period was 49% (S.D. = 19) with a very low, but statistically significant, correlation with the AUC, r = 0.35 (p less than 0.05), thus demonstrating a very great individual variation in sensitivity to cimetidine. In 37 of the patients the time from start of treatment with cimetidine, 1.0 g/day, to disappearance of ulcer symptoms could be assessed, and it was found that neither the individual sensitivity to cimetidine, I%/AUC, nor the gastric secretory capacity, MAO, correlated significantly with the clinical effectiveness of the cimetidine treatment, although 8 patients with a low sensitivity to cimetidine and a high MAO improved more slowly (28 days) than the other 29 patients (13 days) (p less than 0.1)."}
{"id": "PMID:482863", "title": "Nitrofurantoin-induced chronic liver disease. Clinical course and outcome of five cases.", "content": "Adverse liver reactions associated with nitrofurantoin treatment are rare but important complications. Both acute and chronic liver damage have been described. The present report describes five patients who developed chronic liver disease after 1 to 3 years of continued nitrofurantoin treatment. Liver histology was consistent with chronic active hepatitis in four patients, while postnecrotic cirrhosis was observed in one case. Follow-up examinations 2 to 3 years after withdrawal of the drug showed marked improvement clinically and in most cases also histologically.", "contents": "Nitrofurantoin-induced chronic liver disease. Clinical course and outcome of five cases. Adverse liver reactions associated with nitrofurantoin treatment are rare but important complications. Both acute and chronic liver damage have been described. The present report describes five patients who developed chronic liver disease after 1 to 3 years of continued nitrofurantoin treatment. Liver histology was consistent with chronic active hepatitis in four patients, while postnecrotic cirrhosis was observed in one case. Follow-up examinations 2 to 3 years after withdrawal of the drug showed marked improvement clinically and in most cases also histologically."}
{"id": "PMID:482864", "title": "The unstimulated pancreatic secretion obtained by endoscopic cannulation, and the plasma secretin levels in man.", "content": "The technique of collecting juice from the main pancreatic duct by siphonage is described. The juice was collected in 5-min fractions under basal conditions for 20 min (32 subjects) and extended to 60 min (6 subjects). Flow rate and bicarbonate concentration were significantly higher during the first collected samples, whereas concentrations of amylase and protein rose during the first 20 min of the study. All variables remained nearly constant after this period. Immunoreactive secretin (IRS) in peripheral plasma was significantly higher immediately after cannulation of the main pancreatic duct, as compared to the pre-endoscopic level. When the catheter was left in the duct and pancreatic juice drained, the IRS stabilized at a level not significantly different from the pre-endoscopic level. Positive correlations were found between flow rate and plasma level of IRS and between flow rate and bicarbonate concentrations. A negative correlation was found between the flow rate and concentrations of amylase and protein. The higher flow rate, plasma level of IRS, and bicarbonate concentration at the beginning of the examination may be due to the presence of acid and/or bile in the duodenal contents shortly after the endoscope enters the duodenum. Later in the procedure the levels have stabilized, which indicates that little or no acid or bile is passing into the duodenum. It is also concluded that secretin may be one factor responsible for the basal pancreatic secretion in man.", "contents": "The unstimulated pancreatic secretion obtained by endoscopic cannulation, and the plasma secretin levels in man. The technique of collecting juice from the main pancreatic duct by siphonage is described. The juice was collected in 5-min fractions under basal conditions for 20 min (32 subjects) and extended to 60 min (6 subjects). Flow rate and bicarbonate concentration were significantly higher during the first collected samples, whereas concentrations of amylase and protein rose during the first 20 min of the study. All variables remained nearly constant after this period. Immunoreactive secretin (IRS) in peripheral plasma was significantly higher immediately after cannulation of the main pancreatic duct, as compared to the pre-endoscopic level. When the catheter was left in the duct and pancreatic juice drained, the IRS stabilized at a level not significantly different from the pre-endoscopic level. Positive correlations were found between flow rate and plasma level of IRS and between flow rate and bicarbonate concentrations. A negative correlation was found between the flow rate and concentrations of amylase and protein. The higher flow rate, plasma level of IRS, and bicarbonate concentration at the beginning of the examination may be due to the presence of acid and/or bile in the duodenal contents shortly after the endoscope enters the duodenum. Later in the procedure the levels have stabilized, which indicates that little or no acid or bile is passing into the duodenum. It is also concluded that secretin may be one factor responsible for the basal pancreatic secretion in man."}
{"id": "PMID:482865", "title": "Tryptophan metabolism in bladder cancer patients from Copenhagen.", "content": "Urinary excretion before and after a loading dose of tryptophan, plasma pyridoxal phosphate concentration and urinary excretion of 4-pyridoxic acid was studied in 44 male bladder cancer patients from Copenhagen. Six patients (14%) had abnormal tryptophan metabolism, decreased plasma pyridoxal phosphate concentration and in most cases low urinary excretion of 4-pyridoxic acid indicating decreased availability of vitamin B6 or decreased formation of pyridoxal phosphate from vitamin B6. It is concluded that abnormal tryptophan metabolism only plays a minor role for bladder cancer carcinogenesis in Copenhagen. It is, however, stressed that such studies do not rule out the possibility that the aromatic degradation products of tryptophan may be cocarcinogens or promotors as other studies have indicated.", "contents": "Tryptophan metabolism in bladder cancer patients from Copenhagen. Urinary excretion before and after a loading dose of tryptophan, plasma pyridoxal phosphate concentration and urinary excretion of 4-pyridoxic acid was studied in 44 male bladder cancer patients from Copenhagen. Six patients (14%) had abnormal tryptophan metabolism, decreased plasma pyridoxal phosphate concentration and in most cases low urinary excretion of 4-pyridoxic acid indicating decreased availability of vitamin B6 or decreased formation of pyridoxal phosphate from vitamin B6. It is concluded that abnormal tryptophan metabolism only plays a minor role for bladder cancer carcinogenesis in Copenhagen. It is, however, stressed that such studies do not rule out the possibility that the aromatic degradation products of tryptophan may be cocarcinogens or promotors as other studies have indicated."}
{"id": "PMID:482866", "title": "Carcinoembryonic antigen in urine in patients with urothelial carcinoma. An expression for the extent of inflammatory reaction of the urinary tract.", "content": "The concentration of carcinoembryonic antigen (CEA) in urine and serum was determined repeatedly during one year in 213 patients followed because of previously treated urothelial carcinoma of the bladder. The findings were correlated to grade and stage of previously treated tumour, given therapy, recurrence and the cytological evaluation of a midstream urine specimen. During the period of follow up 43 recurrences were clinically observed. With the exception for the content of inflammatory cells no correlation was found between the CEA levels in urine or blood and the parameters studied. Thus CEA in urine and/or serum cannot substitute for cystourethroscopy, urography and exfoliative cytology in the follow-up of patients previously treated for urothelial carcinoma.", "contents": "Carcinoembryonic antigen in urine in patients with urothelial carcinoma. An expression for the extent of inflammatory reaction of the urinary tract. The concentration of carcinoembryonic antigen (CEA) in urine and serum was determined repeatedly during one year in 213 patients followed because of previously treated urothelial carcinoma of the bladder. The findings were correlated to grade and stage of previously treated tumour, given therapy, recurrence and the cytological evaluation of a midstream urine specimen. During the period of follow up 43 recurrences were clinically observed. With the exception for the content of inflammatory cells no correlation was found between the CEA levels in urine or blood and the parameters studied. Thus CEA in urine and/or serum cannot substitute for cystourethroscopy, urography and exfoliative cytology in the follow-up of patients previously treated for urothelial carcinoma."}
{"id": "PMID:482867", "title": "Uraemic arterial disease. An experimental study with special reference to the effect of parathyroidectomy.", "content": "A histophatologic study of uraemic arterial lesions was performed in rats. They were made uraemic by 5/6 kidney resection, and the aorta and peripheral arteries were examined after intervals of up to 36 weeks. The characteristics of the arterial lesions were necrosis of medial smooth muscle cells and, in some cases, calcification of the media. The changes appeared first in the aorta, but after long uraemic periods also in peripheral arteries. The incidence and severity of necrosis and of calcification were assessed separately. Parathyroidectomy largely prevented the development of calcification, and to a lesser degree also smooth muscle cell necrosis. The results suggest that secondary hyperparathyroidism plays an important part in the development of uraemic arterial disease.", "contents": "Uraemic arterial disease. An experimental study with special reference to the effect of parathyroidectomy. A histophatologic study of uraemic arterial lesions was performed in rats. They were made uraemic by 5/6 kidney resection, and the aorta and peripheral arteries were examined after intervals of up to 36 weeks. The characteristics of the arterial lesions were necrosis of medial smooth muscle cells and, in some cases, calcification of the media. The changes appeared first in the aorta, but after long uraemic periods also in peripheral arteries. The incidence and severity of necrosis and of calcification were assessed separately. Parathyroidectomy largely prevented the development of calcification, and to a lesser degree also smooth muscle cell necrosis. The results suggest that secondary hyperparathyroidism plays an important part in the development of uraemic arterial disease."}
{"id": "PMID:482868", "title": "The aortic content of glycosaminoglycans and hydroxyproline in experimental uraemia.", "content": "The effect of experimental renal failure in rats on the various glycosaminoglycan fractions and on the hydroxyproline content of the aorta was studied with special reference to the influence of parathyroidectomy. Renal failure increased those fractions which are known to contain chondroitin sulphate and dermatan sulphate. Parathyroidectomy did not modify these changes. Parathyroidectomy in rats with intact renal function produced an increase of the same fractions. It is concluded that accumulation of sulphated glycosaminoglycans is an early event in the development of uraemic arterial disease. The significance of such accumulation for subsequent development of arterial calcification is discussed. The available data do not permit evaluation of the significance of secondary hyperparathyroidism in the genesis of the observed changes of the glycosaminoglycans.", "contents": "The aortic content of glycosaminoglycans and hydroxyproline in experimental uraemia. The effect of experimental renal failure in rats on the various glycosaminoglycan fractions and on the hydroxyproline content of the aorta was studied with special reference to the influence of parathyroidectomy. Renal failure increased those fractions which are known to contain chondroitin sulphate and dermatan sulphate. Parathyroidectomy did not modify these changes. Parathyroidectomy in rats with intact renal function produced an increase of the same fractions. It is concluded that accumulation of sulphated glycosaminoglycans is an early event in the development of uraemic arterial disease. The significance of such accumulation for subsequent development of arterial calcification is discussed. The available data do not permit evaluation of the significance of secondary hyperparathyroidism in the genesis of the observed changes of the glycosaminoglycans."}
{"id": "PMID:482869", "title": "Morphology of the lymph nodes draining renal adenocarcinoma in relation to immunological functions.", "content": "One hundred and thirty-four lymph nodes from forty-seven patients with renal adenocarcinoma and the same number of regional nodes from sixty-one patients with gastric ulcer were ewwuated histologically by using the standardized reporting system introduced by Cottier, Turk & Sobin (1973). Special attention was focused on the morphologic features which correlate with the immunological responses of these lymph nodes. The cellular elements of the antibody response were found to be active more often in the ulcer series than in the cancer series. On the other hand, the constituents of the cell-mediated immune system were depressed more often in the nodes draining renal carcinomas. The significance of the different immune responses evoked by these two diseases is discussed and the conclusion is drawn that there is some degree of derangement of both humoral and cell-mediated immune systems in the regional lymph nodes of renal adenocarcinoma. The applicability of the reporting system used in assessing the morphology of human lymph nodes is to be emphasized.", "contents": "Morphology of the lymph nodes draining renal adenocarcinoma in relation to immunological functions. One hundred and thirty-four lymph nodes from forty-seven patients with renal adenocarcinoma and the same number of regional nodes from sixty-one patients with gastric ulcer were ewwuated histologically by using the standardized reporting system introduced by Cottier, Turk & Sobin (1973). Special attention was focused on the morphologic features which correlate with the immunological responses of these lymph nodes. The cellular elements of the antibody response were found to be active more often in the ulcer series than in the cancer series. On the other hand, the constituents of the cell-mediated immune system were depressed more often in the nodes draining renal carcinomas. The significance of the different immune responses evoked by these two diseases is discussed and the conclusion is drawn that there is some degree of derangement of both humoral and cell-mediated immune systems in the regional lymph nodes of renal adenocarcinoma. The applicability of the reporting system used in assessing the morphology of human lymph nodes is to be emphasized."}
{"id": "PMID:482871", "title": "Chronic papillomatous dermatitis as a peristomal complication in conduit urinary diversion.", "content": "In 76 patients with conduit urinary diversion, the skin in the stomal area was studied with regard to complications. No such complications had occurred in 39 cases. Superficial erosive dermatitis was present or had been a transient episode in 27 cases. The immediate peristomal area was found in ten cases to show a different type of complication. This was characterized by hyperplastic, papillomatous skin lesions of a chronic nature. A rubber urinary collecting device for long-term use had been fitted in nine of the ten patients, and none of them had a protruding nipple stoma. The clinical, microbiologic and histologic features of these hyperplastic lesions are described and aetiologic factors are discussed. Change to another type of collecting device was followed within three to six weeks by healing of the lesions.", "contents": "Chronic papillomatous dermatitis as a peristomal complication in conduit urinary diversion. In 76 patients with conduit urinary diversion, the skin in the stomal area was studied with regard to complications. No such complications had occurred in 39 cases. Superficial erosive dermatitis was present or had been a transient episode in 27 cases. The immediate peristomal area was found in ten cases to show a different type of complication. This was characterized by hyperplastic, papillomatous skin lesions of a chronic nature. A rubber urinary collecting device for long-term use had been fitted in nine of the ten patients, and none of them had a protruding nipple stoma. The clinical, microbiologic and histologic features of these hyperplastic lesions are described and aetiologic factors are discussed. Change to another type of collecting device was followed within three to six weeks by healing of the lesions."}
{"id": "PMID:482872", "title": "Metastases to the penis from carcinoma of the prostate. A case report.", "content": "In a 68-year-old man metastasis to the penis was diagnosed about two years after transurethral resection of the prostate because of adenocarcinoma. Diethylstilboestrol has been given postoperatively. Initially the metastasis was interpreted as primary cancer of the penis, as biopsy findings indicated a spinocellular carcinoma. Subsequent biopsies, however, showed the same histologic pattern as in the original cancer of the prostate. Up to 1976 about 170 cases of metastasis to the penis were reported in the literature. The prognosis as a rule is very poor. In the present case estramustine phosphate (Estracyt) seemed to improve the patients's general health, but did not affect the penile metastasis. The patient died 18 months after the metastasis had been demonstrated.", "contents": "Metastases to the penis from carcinoma of the prostate. A case report. In a 68-year-old man metastasis to the penis was diagnosed about two years after transurethral resection of the prostate because of adenocarcinoma. Diethylstilboestrol has been given postoperatively. Initially the metastasis was interpreted as primary cancer of the penis, as biopsy findings indicated a spinocellular carcinoma. Subsequent biopsies, however, showed the same histologic pattern as in the original cancer of the prostate. Up to 1976 about 170 cases of metastasis to the penis were reported in the literature. The prognosis as a rule is very poor. In the present case estramustine phosphate (Estracyt) seemed to improve the patients's general health, but did not affect the penile metastasis. The patient died 18 months after the metastasis had been demonstrated."}
{"id": "PMID:482873", "title": "Recurrent bladder papilloma in a child with Cushing's syndrome. Case report.", "content": "A case of bladder papilloma in a 12-year-old boy with Cushing's syndrome was reported by us in 1975. A walnut-sized papilloma was removed by transvesical extirpation, and bilateral adrenalectomy was performed. Two years later he was reoperated because of recurrent Cushing syndrome, and at the same time he had a recurrence of multiple bladder papillomas. Since removal of residual adrenocortical tissue and hormone substitution therapy his Cushing appearance has disappeared completely. The recurrent papillomas were removed by transurethral electrocoagulation and control cystoscopies have shown no sign tumour recurrence. We suggest a possible neoplastic effect of some tryptophan metabolites(s) in combination with immunosuppression as the cause of the bladder papillomas.", "contents": "Recurrent bladder papilloma in a child with Cushing's syndrome. Case report. A case of bladder papilloma in a 12-year-old boy with Cushing's syndrome was reported by us in 1975. A walnut-sized papilloma was removed by transvesical extirpation, and bilateral adrenalectomy was performed. Two years later he was reoperated because of recurrent Cushing syndrome, and at the same time he had a recurrence of multiple bladder papillomas. Since removal of residual adrenocortical tissue and hormone substitution therapy his Cushing appearance has disappeared completely. The recurrent papillomas were removed by transurethral electrocoagulation and control cystoscopies have shown no sign tumour recurrence. We suggest a possible neoplastic effect of some tryptophan metabolites(s) in combination with immunosuppression as the cause of the bladder papillomas."}
{"id": "PMID:482874", "title": "Bladder explosion during uninterrupted transurethral resection of the prostate. A case report and an experimental model.", "content": "A bladder explosion during transurethral resection with intraperitoneal rupture of the bladder is reported. Immediate operative repair was successful and the final result satisfactory. Gas formed during in vitro and in vivo prostatic resections was analysed by means of an oxygen electrode and gascromatographs. Hydrogen was dominant but oxygen and several explosive hydrocarbons were demonstrated. The gas formed in vitro was highly explosive. Oxygen content in gas formed in vivo was considerably lower. Admixture of atmospheric air during transurethral resection increases the risk of explosion and should be avoided and the air pocket in the bladder be kept at an absolute minimum.", "contents": "Bladder explosion during uninterrupted transurethral resection of the prostate. A case report and an experimental model. A bladder explosion during transurethral resection with intraperitoneal rupture of the bladder is reported. Immediate operative repair was successful and the final result satisfactory. Gas formed during in vitro and in vivo prostatic resections was analysed by means of an oxygen electrode and gascromatographs. Hydrogen was dominant but oxygen and several explosive hydrocarbons were demonstrated. The gas formed in vitro was highly explosive. Oxygen content in gas formed in vivo was considerably lower. Admixture of atmospheric air during transurethral resection increases the risk of explosion and should be avoided and the air pocket in the bladder be kept at an absolute minimum."}
{"id": "PMID:482875", "title": "Crossed, non-fused renal ectopia and ipsilateral ureteral carcinoma. A case report.", "content": "A man with crossed, non-fused renal ectopia had carcinoma of the distal ureter, leading to hydro-ureter, hydronephrosis and urinary infection. Crossed ectopic kidneys are more disease-prone than are normal kidneys. Complicating disorders are discussed, the value of thorough urologic investigation is stressed and the incidence of tumour of the upper urinary tract in crossed renal ectopia without fusion is reviewed.", "contents": "Crossed, non-fused renal ectopia and ipsilateral ureteral carcinoma. A case report. A man with crossed, non-fused renal ectopia had carcinoma of the distal ureter, leading to hydro-ureter, hydronephrosis and urinary infection. Crossed ectopic kidneys are more disease-prone than are normal kidneys. Complicating disorders are discussed, the value of thorough urologic investigation is stressed and the incidence of tumour of the upper urinary tract in crossed renal ectopia without fusion is reviewed."}
{"id": "PMID:482876", "title": "Parents of schizophrenic individuals: what we say is what we see.", "content": "The language used to describe the families of schizophrenic individuals is reviewed briefly. It is suggested that this language has negative connotations and is a legacy of past theory interpreting parents as primary causal agents in schizophrenia. If we are to engage the families of schizophrenic patients in cooperative therapeutic endeavors, we must begin to speak to and about those families in more neutral terms.", "contents": "Parents of schizophrenic individuals: what we say is what we see. The language used to describe the families of schizophrenic individuals is reviewed briefly. It is suggested that this language has negative connotations and is a legacy of past theory interpreting parents as primary causal agents in schizophrenia. If we are to engage the families of schizophrenic patients in cooperative therapeutic endeavors, we must begin to speak to and about those families in more neutral terms."}
{"id": "PMID:482877", "title": "Schizoaffective illness defies the dichotomy...and keeps DSM-III pondering.", "content": "Models proposed to account for patients eventually labeled \"schizoaffective\" are traced historically. It is demonstrated that these patients represent a distinct clinical entity, and diagnostic criteria are then applied to reevaluate the position of schizoaffective disorder in our current and future nosology. Schizoaffective illness is held to be the example, par excellence, that defies the validity of Kraepelin's original dichotomy.", "contents": "Schizoaffective illness defies the dichotomy...and keeps DSM-III pondering. Models proposed to account for patients eventually labeled \"schizoaffective\" are traced historically. It is demonstrated that these patients represent a distinct clinical entity, and diagnostic criteria are then applied to reevaluate the position of schizoaffective disorder in our current and future nosology. Schizoaffective illness is held to be the example, par excellence, that defies the validity of Kraepelin's original dichotomy."}
{"id": "PMID:482878", "title": "Overview: the heuristic need for subgroups of the schizophrenic syndrome.", "content": "This article is an editor's introduction to the theme of an issue of the Schizophrenia Bulletin devoted to subgroups of the schizophrenic syndrome. The issue includes articles on subtyping from various points of view: neurological, genetic, biochemical, and descriptive. It is hoped that the wide range of findings being applied in ongoing classificatory efforts will produce subgroups with etiologic, prognostic, and treatment validity.", "contents": "Overview: the heuristic need for subgroups of the schizophrenic syndrome. This article is an editor's introduction to the theme of an issue of the Schizophrenia Bulletin devoted to subgroups of the schizophrenic syndrome. The issue includes articles on subtyping from various points of view: neurological, genetic, biochemical, and descriptive. It is hoped that the wide range of findings being applied in ongoing classificatory efforts will produce subgroups with etiologic, prognostic, and treatment validity."}
{"id": "PMID:482880", "title": "Genetic evidence for the existence of subgroups of the schizophrenic syndrome.", "content": "Evidence for the existence of a genetic factor in the etiology of a significant proportion of the people diagnosed as having a schizophrenic disorder is reviewed. It is suggested that whatever is transmitted genetically need not be inherently pathologic and/or pathogenic. It is argued that only people who have certain trait expressions or phenotypes are capable of a schizophrenic decompensation but that these phenotypes, while genetically loaded, are not necessarily pathogenic. An effort is made to show that even those cases in whom genetic factors operate are not homogeneous but represent separate subgroups which differ in their etiopathogenesis. This etiologic heterogeneity, in the development of a characteristic necessary but not sufficient for a schizophrenic decompensation, will almost certainly be associated with differences in the clinical course of the disorder.", "contents": "Genetic evidence for the existence of subgroups of the schizophrenic syndrome. Evidence for the existence of a genetic factor in the etiology of a significant proportion of the people diagnosed as having a schizophrenic disorder is reviewed. It is suggested that whatever is transmitted genetically need not be inherently pathologic and/or pathogenic. It is argued that only people who have certain trait expressions or phenotypes are capable of a schizophrenic decompensation but that these phenotypes, while genetically loaded, are not necessarily pathogenic. An effort is made to show that even those cases in whom genetic factors operate are not homogeneous but represent separate subgroups which differ in their etiopathogenesis. This etiologic heterogeneity, in the development of a characteristic necessary but not sufficient for a schizophrenic decompensation, will almost certainly be associated with differences in the clinical course of the disorder."}
{"id": "PMID:482882", "title": "An attempted integration of information relevant to schizophrenic subtypes.", "content": "The usefulness and validity of traditional subtypes are questionable. The subtypes described in earlier years no longer emerge with the clarity previously described. The four classical subtypes cannot be reliably distinguished and have not been shown to have predictive validity. Subtypes classified along course or prognostic lines may be more clinically useful. Attempts to subdivide schizophrenia along biologic and genetic lines offer promise. Recent efforts to describe new subdivisions of schizophrenia are readily justified, but new descriptive subtypes are likely to prove useful only when validated by biological, genetic, treatment response, and outcome data.", "contents": "An attempted integration of information relevant to schizophrenic subtypes. The usefulness and validity of traditional subtypes are questionable. The subtypes described in earlier years no longer emerge with the clarity previously described. The four classical subtypes cannot be reliably distinguished and have not been shown to have predictive validity. Subtypes classified along course or prognostic lines may be more clinically useful. Attempts to subdivide schizophrenia along biologic and genetic lines offer promise. Recent efforts to describe new subdivisions of schizophrenia are readily justified, but new descriptive subtypes are likely to prove useful only when validated by biological, genetic, treatment response, and outcome data."}
{"id": "PMID:482883", "title": "Epilogue: subtypes of the schizophrenic syndrome--their current status.", "content": "The expectation that important subtypes exist in dementia praecox (schizophrenia) was built into the earliest conceptualizations of this disorder by Kraepelin and Bleuler. Although the traditional subtypes are still used, more recent biological, psychological, and descriptive-clinical data suggest that quite different approaches to subtyping and subtyping categories may be more valid. No definitive answers to the subtyping problem have yet been reached, but the solution may well involve a complex consideration of biologic, psychologic, and social variables.", "contents": "Epilogue: subtypes of the schizophrenic syndrome--their current status. The expectation that important subtypes exist in dementia praecox (schizophrenia) was built into the earliest conceptualizations of this disorder by Kraepelin and Bleuler. Although the traditional subtypes are still used, more recent biological, psychological, and descriptive-clinical data suggest that quite different approaches to subtyping and subtyping categories may be more valid. No definitive answers to the subtyping problem have yet been reached, but the solution may well involve a complex consideration of biologic, psychologic, and social variables."}
{"id": "PMID:482885", "title": "[Introduction].", "content": "Mental retardation has been much neglected in the past by physicians, especially as far as the clinical, long-term care is concerned. This care can only be adequately performed by a team grouping physician, psychologist, social worker, teacher, educator, etc. The physician must be familiar with the various aspects of mental retardation, including its social implications.", "contents": "[Introduction]. Mental retardation has been much neglected in the past by physicians, especially as far as the clinical, long-term care is concerned. This care can only be adequately performed by a team grouping physician, psychologist, social worker, teacher, educator, etc. The physician must be familiar with the various aspects of mental retardation, including its social implications."}
{"id": "PMID:482886", "title": "[The concept of mental deficiency. Evolution of theories, attitudes and practices].", "content": "In the last twenty years a deep renewal of the conceptions about and the treatments of the mentally deficients has taken place. In an attempt to clarify somehow the confusion in the communication between specialists, the author reminds the classical position on this subject, which relies upon the definition of the idiocy: a state of a severe mental defect, set up early in life, with an organic cause and being incurable for this reason. In spite of the later considerable differenciation at different levels and types, the classical position maintains this definition as the ground of the nosographic class of the mental retardations (debility is considered then as a light idiocy), divided in two sub-classes of either \"polygenic heredity\" or accidents causing early brain damages. From a clinical point of view, such a theoretical position is difficult to apply, especially in cases of middle and light mental defects. From a theoretical point of view, this classical position has been often attacked: by the evidence of the socio-cultural factors of the intellectual development, by the analysis of the so-called instrumental troubles, by the studies about the early lack of mother-care and the child-psychoses with a defect, by the more recent works on dysharmonic evolvements, etc. All this has lead to major theoretical changes which force to give up the illusion of a \"single class\" of mental retardations and study therefore each case in the trajectory of its development, not only of the cognitive patterns but also of the whole personality structure. This clinical approach forces to admit organic, environmental (sociological and psycho-sociological) and relational, dynamic factors as well; and, to accept that their combination, replaced in a genetic context of the functioning patterns, establishes a new, properly psychological level of causality. So, even the idea of a \"defectology\" is non-sense; the factors and the problems in question are the same as in any other psychogenesis. Nevertheless, the study of cases, in which this psychogenesis is caracterized by a mental deficit, is very important.", "contents": "[The concept of mental deficiency. Evolution of theories, attitudes and practices]. In the last twenty years a deep renewal of the conceptions about and the treatments of the mentally deficients has taken place. In an attempt to clarify somehow the confusion in the communication between specialists, the author reminds the classical position on this subject, which relies upon the definition of the idiocy: a state of a severe mental defect, set up early in life, with an organic cause and being incurable for this reason. In spite of the later considerable differenciation at different levels and types, the classical position maintains this definition as the ground of the nosographic class of the mental retardations (debility is considered then as a light idiocy), divided in two sub-classes of either \"polygenic heredity\" or accidents causing early brain damages. From a clinical point of view, such a theoretical position is difficult to apply, especially in cases of middle and light mental defects. From a theoretical point of view, this classical position has been often attacked: by the evidence of the socio-cultural factors of the intellectual development, by the analysis of the so-called instrumental troubles, by the studies about the early lack of mother-care and the child-psychoses with a defect, by the more recent works on dysharmonic evolvements, etc. All this has lead to major theoretical changes which force to give up the illusion of a \"single class\" of mental retardations and study therefore each case in the trajectory of its development, not only of the cognitive patterns but also of the whole personality structure. This clinical approach forces to admit organic, environmental (sociological and psycho-sociological) and relational, dynamic factors as well; and, to accept that their combination, replaced in a genetic context of the functioning patterns, establishes a new, properly psychological level of causality. So, even the idea of a \"defectology\" is non-sense; the factors and the problems in question are the same as in any other psychogenesis. Nevertheless, the study of cases, in which this psychogenesis is caracterized by a mental deficit, is very important."}
{"id": "PMID:482887", "title": "[The physician faced with the mentally handicapped and his family].", "content": "In his work in the field of mental retardation, the physician is exposed to his vivid emotive reactions. He risks either to withdraw in a scientific relation or to identify himself with either family's members. The physician must learn to join with a pluridisciplinary team, to build up his emotional impulses, to answer to the needs of the mental handicapped and his family and to estimate the evolutive potential rather than the deficits. He must be conscious that the handicapped and his family are called, in an imperative manner, to resolve many tasks: they must not only confront themselves with many specialists (doctors, teachers, educators, logopedists, physiotherapists) and many Services, but also overcome the shock of the diagnosis and build, on the existential plan, a new life.", "contents": "[The physician faced with the mentally handicapped and his family]. In his work in the field of mental retardation, the physician is exposed to his vivid emotive reactions. He risks either to withdraw in a scientific relation or to identify himself with either family's members. The physician must learn to join with a pluridisciplinary team, to build up his emotional impulses, to answer to the needs of the mental handicapped and his family and to estimate the evolutive potential rather than the deficits. He must be conscious that the handicapped and his family are called, in an imperative manner, to resolve many tasks: they must not only confront themselves with many specialists (doctors, teachers, educators, logopedists, physiotherapists) and many Services, but also overcome the shock of the diagnosis and build, on the existential plan, a new life."}
{"id": "PMID:482888", "title": "[Dysplastic gangliocytoma of the cerebellum].", "content": "A case of dysplastic gangliocytoma of the cerebellum (Lhermitte-Duclos disease) is reported, discovered on a routine autopsy. Clinical and anatomopathological data of the case are compared with previously reported cases. Review of the various proposed pathogenic hypotheses.", "contents": "[Dysplastic gangliocytoma of the cerebellum]. A case of dysplastic gangliocytoma of the cerebellum (Lhermitte-Duclos disease) is reported, discovered on a routine autopsy. Clinical and anatomopathological data of the case are compared with previously reported cases. Review of the various proposed pathogenic hypotheses."}
{"id": "PMID:482889", "title": "[Mental deficiency: organic aspects].", "content": "This review of the organic aspects of mental retardation considers this handicap among the other chronic neuropediatric disorders and is one of the numerous possible manifestations of a cerebral damage occurring in a developing nervous system. The relative frequency of the different etiologies is discussed in relation to the severity of the mental handicap. The prenatal causes of mental deficiency which are the most frequent are reviewed and a distinction is made between malformations and pseudomalformations. Particular mention of the fetal alcohol syndrome is made. The role of perinatal medicine in the epidemiology of mental retardation is discussed and the diagnostic approach of a particular child with mental retardation is presented with the personal experience of the author in the pediatric department, CHUV, Lausanne. The possible measures to prevent mental retardation are finally discussed.", "contents": "[Mental deficiency: organic aspects]. This review of the organic aspects of mental retardation considers this handicap among the other chronic neuropediatric disorders and is one of the numerous possible manifestations of a cerebral damage occurring in a developing nervous system. The relative frequency of the different etiologies is discussed in relation to the severity of the mental handicap. The prenatal causes of mental deficiency which are the most frequent are reviewed and a distinction is made between malformations and pseudomalformations. Particular mention of the fetal alcohol syndrome is made. The role of perinatal medicine in the epidemiology of mental retardation is discussed and the diagnostic approach of a particular child with mental retardation is presented with the personal experience of the author in the pediatric department, CHUV, Lausanne. The possible measures to prevent mental retardation are finally discussed."}
{"id": "PMID:482890", "title": "[Judicial aspect of mental deficiency].", "content": "The author examines the different laws on mental deficiency with particular references to protective measures such as: different forms of guardianship and legal advice. The provisions of the laws on guardianship which do offer an effectual protection, often remain unrecognized.", "contents": "[Judicial aspect of mental deficiency]. The author examines the different laws on mental deficiency with particular references to protective measures such as: different forms of guardianship and legal advice. The provisions of the laws on guardianship which do offer an effectual protection, often remain unrecognized."}
{"id": "PMID:482891", "title": "[Curative-pedagogic aspects of mental deficiency].", "content": "Curative pedagogy is nothing else but a theory on education and teaching of the mentally handicapped. The author presents the following seven thesis: 1. Mental deficiency is not a definite state. It should be considered as a \"relative\" state, bound to its origins but with possibilities of change. It is thus to be considered as a state that can be influenced, modified and within the reach of education. 2. The notion that every child can be educated is inherent to pedagogic theory. The mentally deficient child forces us to examine, more often, our teaching methods. 3. It is only when the doctor and the pedagogue seriously collaborate that it becomes possible to elaborate early developmental programmes. 4. The pedagogue does not remain centered on diagnosis, but allows himself to be directed by the developmental possibilities of the child. 5. Pedagogy is not only concerned with the impairment of intelligence, but seeks a global approach in which the affective relationship is taken into account. This global approach is possible only with a multidisciplinary team. 6. Pedagogy takes into account the parents as well as the child in order to assist them and help them accept the situation. 7. Pedagogic help should not terminate with the end of official schooling, but should remain a permanent support which should be continuously improved.", "contents": "[Curative-pedagogic aspects of mental deficiency]. Curative pedagogy is nothing else but a theory on education and teaching of the mentally handicapped. The author presents the following seven thesis: 1. Mental deficiency is not a definite state. It should be considered as a \"relative\" state, bound to its origins but with possibilities of change. It is thus to be considered as a state that can be influenced, modified and within the reach of education. 2. The notion that every child can be educated is inherent to pedagogic theory. The mentally deficient child forces us to examine, more often, our teaching methods. 3. It is only when the doctor and the pedagogue seriously collaborate that it becomes possible to elaborate early developmental programmes. 4. The pedagogue does not remain centered on diagnosis, but allows himself to be directed by the developmental possibilities of the child. 5. Pedagogy is not only concerned with the impairment of intelligence, but seeks a global approach in which the affective relationship is taken into account. This global approach is possible only with a multidisciplinary team. 6. Pedagogy takes into account the parents as well as the child in order to assist them and help them accept the situation. 7. Pedagogic help should not terminate with the end of official schooling, but should remain a permanent support which should be continuously improved."}
{"id": "PMID:482892", "title": "[The place of institutions for the mentally deficient].", "content": "The institutions, more and more adjusted to the age and the specific handicap of the mentally deficients, attempt to provide an harmonious education. In order to do so, they have to resolve many problems: problems of the handicapped children, who are confronted to a new and very different life-style as well as to a large number of adults who will be taking care of them; problems of the parents, who are badly informed about the institutional methods; problems of the public to whom the existence of the medicoeducational centers gives good conscience; problems of the staff, called to work in a pluridisciplinary team and whose effeciency depends on its cohesion. Such teams should also include the parents.", "contents": "[The place of institutions for the mentally deficient]. The institutions, more and more adjusted to the age and the specific handicap of the mentally deficients, attempt to provide an harmonious education. In order to do so, they have to resolve many problems: problems of the handicapped children, who are confronted to a new and very different life-style as well as to a large number of adults who will be taking care of them; problems of the parents, who are badly informed about the institutional methods; problems of the public to whom the existence of the medicoeducational centers gives good conscience; problems of the staff, called to work in a pluridisciplinary team and whose effeciency depends on its cohesion. Such teams should also include the parents."}
{"id": "PMID:482893", "title": "[Parents of the mentally retarded].", "content": "The author is expressing the point of view of the parents of mentally handicapped children or adults. Taking charge of handicapped people means that measures appropriate to each case will be taken such as the adequate and repeated information of parents not prepared for the inevitable psychological shock of the birth of a handicapped child. The parents go through several phases in their reactions to this situation, which are recognized by psychiatrists. The collaboration of specialists and families is indispensable to the healthy development of handicapped people of all ages. Treatment requires periodic multi-disciplinary check-ups to foster the best possible development of the remaining faculties.", "contents": "[Parents of the mentally retarded]. The author is expressing the point of view of the parents of mentally handicapped children or adults. Taking charge of handicapped people means that measures appropriate to each case will be taken such as the adequate and repeated information of parents not prepared for the inevitable psychological shock of the birth of a handicapped child. The parents go through several phases in their reactions to this situation, which are recognized by psychiatrists. The collaboration of specialists and families is indispensable to the healthy development of handicapped people of all ages. Treatment requires periodic multi-disciplinary check-ups to foster the best possible development of the remaining faculties."}
{"id": "PMID:482894", "title": "[Neuro-ophthalmological problems: the diagnostic value of contrast sensitivity].", "content": "The evaluation of the individual contrast sensitivity function may prove useful in cases affected by lesions of the optic pathways, even when the usual neuro-ophthalmological tests (i.e. visual acuity, visual field examination) are normal. The contrast sensitivity function represents an alternative method to assess the power of spatial resolution of the visual system: in particular, it enables to measure the sensitivity at high, medium and low ranges of spatial frequencies, this being unique to such a procedure. The possible physiological mechanisms underlying the contrast sensitivity function as well as its pathological changes are reviewed.", "contents": "[Neuro-ophthalmological problems: the diagnostic value of contrast sensitivity]. The evaluation of the individual contrast sensitivity function may prove useful in cases affected by lesions of the optic pathways, even when the usual neuro-ophthalmological tests (i.e. visual acuity, visual field examination) are normal. The contrast sensitivity function represents an alternative method to assess the power of spatial resolution of the visual system: in particular, it enables to measure the sensitivity at high, medium and low ranges of spatial frequencies, this being unique to such a procedure. The possible physiological mechanisms underlying the contrast sensitivity function as well as its pathological changes are reviewed."}
{"id": "PMID:482895", "title": "[Transsexualism and its therapy].", "content": "After a review of the historical development of ideas about transsexualiam and the discussion of possible somatic and psychological causes of this condition, the results of explorations of 12 male and 6 female transsexuals who desired a hormonal and surgical approximation to the opposite sex are reported. Two of the nine patients whose chromosomes were examined were found to suffer from Klinefelter's Syndrome. From the personal history it was apparent that 8 of the 12 male transsexuals had difficulty in identifying with an absent, alcoholic or brutal father. The wish to be a member of the opposite sex could be traced in 10 of the 12 male and all 6 female transsexuals to the first five years of life. 7 of the 12 male transsexuals and 3 of the 6 female transsexuals were found to have attempted suicide or to have had suicidal tendencies in the past. In 9/12 male transsexuals and 5/6 female transsexuals the \"change of sex\" operation was considered to be indicated. The average age at operation was 28 for the male transsexuals and 26 for the female transsexuals. The follow-up studies were done 2 months to 3 years after operation for the male transsexuals (average 1, 7 years) and 2 to 3 3/4 years post operatively (average 2, 9 years) for the female transsexuals. 7/8 of the male transsexuals from whom we have katamneses and both female transsexuals reported that they felt subjectively better than before the operation. The present legal situation concerning the registration of change of name and sex in various countries is described.", "contents": "[Transsexualism and its therapy]. After a review of the historical development of ideas about transsexualiam and the discussion of possible somatic and psychological causes of this condition, the results of explorations of 12 male and 6 female transsexuals who desired a hormonal and surgical approximation to the opposite sex are reported. Two of the nine patients whose chromosomes were examined were found to suffer from Klinefelter's Syndrome. From the personal history it was apparent that 8 of the 12 male transsexuals had difficulty in identifying with an absent, alcoholic or brutal father. The wish to be a member of the opposite sex could be traced in 10 of the 12 male and all 6 female transsexuals to the first five years of life. 7 of the 12 male transsexuals and 3 of the 6 female transsexuals were found to have attempted suicide or to have had suicidal tendencies in the past. In 9/12 male transsexuals and 5/6 female transsexuals the \"change of sex\" operation was considered to be indicated. The average age at operation was 28 for the male transsexuals and 26 for the female transsexuals. The follow-up studies were done 2 months to 3 years after operation for the male transsexuals (average 1, 7 years) and 2 to 3 3/4 years post operatively (average 2, 9 years) for the female transsexuals. 7/8 of the male transsexuals from whom we have katamneses and both female transsexuals reported that they felt subjectively better than before the operation. The present legal situation concerning the registration of change of name and sex in various countries is described."}
{"id": "PMID:482896", "title": "[Psychiatric prognosis in epilepsies].", "content": "It was illustrated by examples how psychical disturbances of epileptics can be used as indicators a) for brain lesions which cause the epilepsy too, b) for epileptic activity of the brain itself, and c) for the consequences of epilepsies of long duration. Furthermore, how they can be used as predictors for the success of the epileptic during his life. This course-related arrangement of psychical disturbances which predict a negative prognosis corresponds with measures for prevention of the development of psychical disturbances, for therapeutic diminishing or removal of existing psychical disturbances, and for prophylaxis (or tertiary prevention resp.) of negative psychical consequences or relapses of epilepsy. These measures should improve the life-success of the epileptic patient.", "contents": "[Psychiatric prognosis in epilepsies]. It was illustrated by examples how psychical disturbances of epileptics can be used as indicators a) for brain lesions which cause the epilepsy too, b) for epileptic activity of the brain itself, and c) for the consequences of epilepsies of long duration. Furthermore, how they can be used as predictors for the success of the epileptic during his life. This course-related arrangement of psychical disturbances which predict a negative prognosis corresponds with measures for prevention of the development of psychical disturbances, for therapeutic diminishing or removal of existing psychical disturbances, and for prophylaxis (or tertiary prevention resp.) of negative psychical consequences or relapses of epilepsy. These measures should improve the life-success of the epileptic patient."}
{"id": "PMID:482897", "title": "[Hydrocephalic psychopathy. Psychopathology and clinical symptoms in organic psychosyndrome].", "content": "In reviewing the literature on the psychopathology \"of chronic hydrocephalic psychopathy\" attention is called to its complexity which includes also lack of any symptoms. Nevertheless a group can be distinguished showing affective disorders of diencephalic type. Glaus and Walther-B\u00fcel examined patients with disturbances of mood and energy due to hydrocephaly who were ascribed to the psychosyndrome of local organic brain foundation after M. Bleuler; according to the aeromegaloid psychopaths they were called \"hydrocephalic psychopaths\". A new case is discussed who presented a small-delinquency for years with long-during forensic consequences before the discovery of a marked hydrocephaly of unknown etiology; relapses could be avoided by compensation of the forensic measures by medical and psychoagogic care. The difficulties of diagnosis are shown with emphasis on neuroradiologic clarification in suspect cases.", "contents": "[Hydrocephalic psychopathy. Psychopathology and clinical symptoms in organic psychosyndrome]. In reviewing the literature on the psychopathology \"of chronic hydrocephalic psychopathy\" attention is called to its complexity which includes also lack of any symptoms. Nevertheless a group can be distinguished showing affective disorders of diencephalic type. Glaus and Walther-B\u00fcel examined patients with disturbances of mood and energy due to hydrocephaly who were ascribed to the psychosyndrome of local organic brain foundation after M. Bleuler; according to the aeromegaloid psychopaths they were called \"hydrocephalic psychopaths\". A new case is discussed who presented a small-delinquency for years with long-during forensic consequences before the discovery of a marked hydrocephaly of unknown etiology; relapses could be avoided by compensation of the forensic measures by medical and psychoagogic care. The difficulties of diagnosis are shown with emphasis on neuroradiologic clarification in suspect cases."}
{"id": "PMID:482898", "title": "Mortality among Finnish doctors, 1953-1972.", "content": "The mortality with respect to the total population of Finnish physicians during the period 1953 to 1972 has been analysed and compared with the corresponding statistics for the general Finnish population, for Finnish foundry workers, and for American physicians. It was found that the overall mortality was lower for male physicians than that for the general population or for foundry workers, but was clearly higher than that for American physicians. Male physicians did not exhibit any major differences from the general population with regard to cardiovascular diseases and suicide, but had a lower mortality from malignant neoplasms, accidents and \"other diseases\" (including infectious diseases). The explanation of cancer mortality being lower than expected among male physicians was mainly to be found in a deficit in lung cancer. Although female physicians had higher life expectancy than male physicians and the female general Finnish population, they did not show any clear deficit for cancer. In respect of all specialists, surgical specialists had the lowest mortality; general practitioners had the highest mortality. Most of these variations were attributable to differences in coronary mortality, but mortality from lung cancer was also remarkably low among surgeons. Differences of a similar type were also found between occupational sub-categories; private practitioners had the highest, and research workers and central hospital physicians the lowest mortality figures. The lower cancer mortality among male physicians, as contrasted with the general population, is probably attributable to differences in smoking habits; about 22% of male physicians smoked in 1973, whereas earlier studies by others have indicated that the corresponding proportion was about 50% in the general population. In contrast, the differences in mortality between different specialist categories probably arises from other factors, since Finnish physicians reportedly display a relatively homogeneous smoking pattern.", "contents": "Mortality among Finnish doctors, 1953-1972. The mortality with respect to the total population of Finnish physicians during the period 1953 to 1972 has been analysed and compared with the corresponding statistics for the general Finnish population, for Finnish foundry workers, and for American physicians. It was found that the overall mortality was lower for male physicians than that for the general population or for foundry workers, but was clearly higher than that for American physicians. Male physicians did not exhibit any major differences from the general population with regard to cardiovascular diseases and suicide, but had a lower mortality from malignant neoplasms, accidents and \"other diseases\" (including infectious diseases). The explanation of cancer mortality being lower than expected among male physicians was mainly to be found in a deficit in lung cancer. Although female physicians had higher life expectancy than male physicians and the female general Finnish population, they did not show any clear deficit for cancer. In respect of all specialists, surgical specialists had the lowest mortality; general practitioners had the highest mortality. Most of these variations were attributable to differences in coronary mortality, but mortality from lung cancer was also remarkably low among surgeons. Differences of a similar type were also found between occupational sub-categories; private practitioners had the highest, and research workers and central hospital physicians the lowest mortality figures. The lower cancer mortality among male physicians, as contrasted with the general population, is probably attributable to differences in smoking habits; about 22% of male physicians smoked in 1973, whereas earlier studies by others have indicated that the corresponding proportion was about 50% in the general population. In contrast, the differences in mortality between different specialist categories probably arises from other factors, since Finnish physicians reportedly display a relatively homogeneous smoking pattern."}
{"id": "PMID:482899", "title": "Effect of loneliness on mortality after retirement.", "content": "In the present study, the effect of the type and number of available objects on post-retirement mortality was examined. The hypothesis is that the fewer available objects people have upon retirement, the greater will be the mortality after the retirement. The material was divided into three groups: married (assumed to have the greatest object potential), widowed or divorced (second greatest), and single (least potential). The material consisted of men retired on old age pension in 1964. Of these, 792 were married, 119 were widowed or divorced, and 77 were single. No statistically significant differences in mortality between groups on the basis of marital status could be found, although the results tended to favour the hypothesis. The number of suicides and accidents in the group of men dying within two years of retirement exceeded the expected values, but this increase was distributed fairly evenly between groups on the basis of marital status.", "contents": "Effect of loneliness on mortality after retirement. In the present study, the effect of the type and number of available objects on post-retirement mortality was examined. The hypothesis is that the fewer available objects people have upon retirement, the greater will be the mortality after the retirement. The material was divided into three groups: married (assumed to have the greatest object potential), widowed or divorced (second greatest), and single (least potential). The material consisted of men retired on old age pension in 1964. Of these, 792 were married, 119 were widowed or divorced, and 77 were single. No statistically significant differences in mortality between groups on the basis of marital status could be found, although the results tended to favour the hypothesis. The number of suicides and accidents in the group of men dying within two years of retirement exceeded the expected values, but this increase was distributed fairly evenly between groups on the basis of marital status."}
{"id": "PMID:482900", "title": "Application of diagnostic criteria in the diagnosis of myocardial infarction.", "content": "A random sample of all patients with a registered diagnosis of acute myocardial infarction (AMI) was selected from the hospitalisation register of Stockholm County in 1973. The sample consisted of 269 patients. The medical records of these patients were scrutinized concerning the fulfilment of criteria of a diagnosis of AMI. The criteria of AMI chosen were those established in a Sweden cooperative study (6). 33 patients did not fulfil the diagnosis of AMI, corresponding to an estimated proportion of 10%. In 4% this was due to more technical reasons such as the use of incorrect code numbers, and in 6% the diagnosis had been made without the criteria being fulfilled.", "contents": "Application of diagnostic criteria in the diagnosis of myocardial infarction. A random sample of all patients with a registered diagnosis of acute myocardial infarction (AMI) was selected from the hospitalisation register of Stockholm County in 1973. The sample consisted of 269 patients. The medical records of these patients were scrutinized concerning the fulfilment of criteria of a diagnosis of AMI. The criteria of AMI chosen were those established in a Sweden cooperative study (6). 33 patients did not fulfil the diagnosis of AMI, corresponding to an estimated proportion of 10%. In 4% this was due to more technical reasons such as the use of incorrect code numbers, and in 6% the diagnosis had been made without the criteria being fulfilled."}
{"id": "PMID:482901", "title": "Work and disability at the age of 30 years. A sociomedical study of a birth-cohort from Bergen. V. Social background and recruitment to the school system.", "content": "The basis of the present study is a cohort of 1570 persons, all live births in 1940 of mothers then residing in Bergen. This birth-control was followed up in the compulsory school system at the age of 14 years. Information from the \"parsons' lists\" (birth registers) was gathered concerning the parents' social background, while facts about the students' recruitment to the compulsory school system in Bergen were obtained from the local files of the various schools and the files of The National Services for the Mentally Retarded. For the purpose of collecting more detailed information, a sample was taken from the birthcohort. This sample was formed on the basis of a stratification of the cohort according to type of school attended at 14 years of age. By supplementing the group comprising persons attending Special Schools for the Educable Mentally Retarded and the group including persons cared for by the National Services for the Mentally Retarded, a total sample of 262 persons was reached. It is found that recruitment to the school system varies considerably with socio-economic background. Children of higher officials were highly over-represented in Junior High School, while children of workers were noticeably under-represented at this type of school. Children of workers were over-represented in Continuation School, Elementary School classes for slow learners and Special Schools for the educable mentally retarded. However, a proportionally very similar representation of the social groups in the services for the mentally retarded was found.", "contents": "Work and disability at the age of 30 years. A sociomedical study of a birth-cohort from Bergen. V. Social background and recruitment to the school system. The basis of the present study is a cohort of 1570 persons, all live births in 1940 of mothers then residing in Bergen. This birth-control was followed up in the compulsory school system at the age of 14 years. Information from the \"parsons' lists\" (birth registers) was gathered concerning the parents' social background, while facts about the students' recruitment to the compulsory school system in Bergen were obtained from the local files of the various schools and the files of The National Services for the Mentally Retarded. For the purpose of collecting more detailed information, a sample was taken from the birthcohort. This sample was formed on the basis of a stratification of the cohort according to type of school attended at 14 years of age. By supplementing the group comprising persons attending Special Schools for the Educable Mentally Retarded and the group including persons cared for by the National Services for the Mentally Retarded, a total sample of 262 persons was reached. It is found that recruitment to the school system varies considerably with socio-economic background. Children of higher officials were highly over-represented in Junior High School, while children of workers were noticeably under-represented at this type of school. Children of workers were over-represented in Continuation School, Elementary School classes for slow learners and Special Schools for the educable mentally retarded. However, a proportionally very similar representation of the social groups in the services for the mentally retarded was found."}
{"id": "PMID:482902", "title": "Inverse association between risk factors for benign and malignant breast lesions.", "content": "The purpose of the present study was to compare the risk of breast cancer and the risk of benign breast disease using known risk factors for breast cancer. The series was taken during breast cancer screening of women aged 41-60 in an industrial city in Finland. 158 breast lesions were diagnosed, 27 of which were malignant. Women with breast disease and 534 controls were interviewed to obtain epidemiological data. The prevalence of benign lesions decreased after menopause but the prevalence of carcinomas was essentially the same over the age span 41-60. Several risk factors for breast cancer, such as selected reproductive and hormonal characteristics, were not associated with the risk of benign breast disease. Thus it was concluded that benign and malignant breast lesions are not associated in general, and the decrease in the prevalence of benign breast lesions after menopause is more likely to be due to regression than to transition to carcinoma.", "contents": "Inverse association between risk factors for benign and malignant breast lesions. The purpose of the present study was to compare the risk of breast cancer and the risk of benign breast disease using known risk factors for breast cancer. The series was taken during breast cancer screening of women aged 41-60 in an industrial city in Finland. 158 breast lesions were diagnosed, 27 of which were malignant. Women with breast disease and 534 controls were interviewed to obtain epidemiological data. The prevalence of benign lesions decreased after menopause but the prevalence of carcinomas was essentially the same over the age span 41-60. Several risk factors for breast cancer, such as selected reproductive and hormonal characteristics, were not associated with the risk of benign breast disease. Thus it was concluded that benign and malignant breast lesions are not associated in general, and the decrease in the prevalence of benign breast lesions after menopause is more likely to be due to regression than to transition to carcinoma."}
{"id": "PMID:482903", "title": "A multiphasic screening programme at the health centre level: the S\u00e4kyl\u00e4-K\u00f6yli\u00f6 project.", "content": "The S\u00e4kyl\u00e4-K\u00f6yli\u00f6 Project concerned the investigation and evaluation of different screening programmes and the possibilities for providing the necessary treatment and follow-up at the health centre level. The variables studied consisted of anaemia, bacteriuria, high blood pressure, and mental health problems; an epidemiologic screening for sight and hearing defects and diseases of the musculoskeletal system was carried out as well. The use of health services, together with the relationships between objectively and subjectively determined morbidity, was also studied. A total of 2268 inhabitants of the municipalities of S\u00e4kyl\u00e4 and K\u00f6yli\u00f6, i.e. 93.2% of the 40- to 64-year-old female population and 93.4% of the males in the same age range, participated in the screening programme conducted by the S\u00e4kyl\u00e4-K\u00f6yli\u00f6 Project between 9 September 1973 and 11 January 1974. Only one-fifth (1.9%) of the non-respondents to the first invitation (8.6%) participated in the screening programme after they received a second invitation. This result demonstrates that those who do not participate for some reason cannot, to any great extent, be influenced by a renewed invitation. Since the non-participation rate was insignificant, the material examined may, from the epidemiologic point of view, be considered as representative of the middle-aged population of the two municipalities, which together form a health centre district in Southwest Finland. The high participation percentage also shows that the prerequisite of screening, namely, a positive attitude on the part of the population, was as well fulfilled in the present project.", "contents": "A multiphasic screening programme at the health centre level: the S\u00e4kyl\u00e4-K\u00f6yli\u00f6 project. The S\u00e4kyl\u00e4-K\u00f6yli\u00f6 Project concerned the investigation and evaluation of different screening programmes and the possibilities for providing the necessary treatment and follow-up at the health centre level. The variables studied consisted of anaemia, bacteriuria, high blood pressure, and mental health problems; an epidemiologic screening for sight and hearing defects and diseases of the musculoskeletal system was carried out as well. The use of health services, together with the relationships between objectively and subjectively determined morbidity, was also studied. A total of 2268 inhabitants of the municipalities of S\u00e4kyl\u00e4 and K\u00f6yli\u00f6, i.e. 93.2% of the 40- to 64-year-old female population and 93.4% of the males in the same age range, participated in the screening programme conducted by the S\u00e4kyl\u00e4-K\u00f6yli\u00f6 Project between 9 September 1973 and 11 January 1974. Only one-fifth (1.9%) of the non-respondents to the first invitation (8.6%) participated in the screening programme after they received a second invitation. This result demonstrates that those who do not participate for some reason cannot, to any great extent, be influenced by a renewed invitation. Since the non-participation rate was insignificant, the material examined may, from the epidemiologic point of view, be considered as representative of the middle-aged population of the two municipalities, which together form a health centre district in Southwest Finland. The high participation percentage also shows that the prerequisite of screening, namely, a positive attitude on the part of the population, was as well fulfilled in the present project."}
{"id": "PMID:482904", "title": "Physical work capacity of young men in Sweden.", "content": "Each year between 1961 and 1975 all male students in a secondary school were submaximally tested on several occasions on bicycle ergometers with regard to estimated maximal aerobic power. The school is situated in central Sweden (Falun). The students were between 16 and 20 years of age. Data from the tests in Falun and data compiled from other sources cannot confirm the hypothesis that young men of today have a lower maximal aerobic power than young men of the 1960s. The results indicate that Swedish boys aged 16 to 20 have, on average, a maximal aerobic power of about 45 ml O2 X (kg x min)-1. The standard deviation is about 8 ml O2.", "contents": "Physical work capacity of young men in Sweden. Each year between 1961 and 1975 all male students in a secondary school were submaximally tested on several occasions on bicycle ergometers with regard to estimated maximal aerobic power. The school is situated in central Sweden (Falun). The students were between 16 and 20 years of age. Data from the tests in Falun and data compiled from other sources cannot confirm the hypothesis that young men of today have a lower maximal aerobic power than young men of the 1960s. The results indicate that Swedish boys aged 16 to 20 have, on average, a maximal aerobic power of about 45 ml O2 X (kg x min)-1. The standard deviation is about 8 ml O2."}
{"id": "PMID:482906", "title": "[Intravascular coagulation: symptom or disease?].", "content": "The laboratory pattern of intravascular coagulation is the result of complex mechanisms. The accelerated disappearance of substrates may be partially or completely compensated by increased production, depending on the integrity of liver and bone marrow. Ischemic complications are to be expected almost exclusively in acute cases, because of efficient mechanisms for the elimination of fibrin and other products. The typical laboratory pattern may be produced by loss of plasma from the circulation and extravasal proteolysis of the coagulation substrates. Chronic intravascular coagulation may be compensated, with resultant hyper-coagulability and increased risk of thromboembolic complications.", "contents": "[Intravascular coagulation: symptom or disease?]. The laboratory pattern of intravascular coagulation is the result of complex mechanisms. The accelerated disappearance of substrates may be partially or completely compensated by increased production, depending on the integrity of liver and bone marrow. Ischemic complications are to be expected almost exclusively in acute cases, because of efficient mechanisms for the elimination of fibrin and other products. The typical laboratory pattern may be produced by loss of plasma from the circulation and extravasal proteolysis of the coagulation substrates. Chronic intravascular coagulation may be compensated, with resultant hyper-coagulability and increased risk of thromboembolic complications."}
{"id": "PMID:482908", "title": "[Transcobalamine-II-polymorphism: biochemical and clinical aspects of rare variants].", "content": "Transcobalamin II(TC II) is an essential transport protein for vitamin B12 in blood. TC II can be split up into isoproteins by polyacrylamide gel electrophoresis. Family studies are compatible with a genetic polymorphism of TC II and a five allele system. Screening of TC II isoprotein patterns in about 1000 individuals yielded two unusual TC II variants: The first case was a black female with severe megaloblastic anemia since infancy. Her TC II was elevated and bound B12, but displayed markedly diminished functional capacity to transfer radioactive cyanocobalamin in cellular systems. Comparison of this patient's TC II isoprotein pattern with known variants showed a distinct difference in electrophoretic mobility, indicating the presence of a sixth allele. The second patient, also presenting with pernicious anemia-like symptoms, was found to possess an unusual TC II variant with reduced TC II serum levels. Corresponding variants were also observed in the patient's asymptomatic children. Thus, abnormal TC II variants probably causing megaloblastic anemias both correlated with unusual isoprotein patterns.", "contents": "[Transcobalamine-II-polymorphism: biochemical and clinical aspects of rare variants]. Transcobalamin II(TC II) is an essential transport protein for vitamin B12 in blood. TC II can be split up into isoproteins by polyacrylamide gel electrophoresis. Family studies are compatible with a genetic polymorphism of TC II and a five allele system. Screening of TC II isoprotein patterns in about 1000 individuals yielded two unusual TC II variants: The first case was a black female with severe megaloblastic anemia since infancy. Her TC II was elevated and bound B12, but displayed markedly diminished functional capacity to transfer radioactive cyanocobalamin in cellular systems. Comparison of this patient's TC II isoprotein pattern with known variants showed a distinct difference in electrophoretic mobility, indicating the presence of a sixth allele. The second patient, also presenting with pernicious anemia-like symptoms, was found to possess an unusual TC II variant with reduced TC II serum levels. Corresponding variants were also observed in the patient's asymptomatic children. Thus, abnormal TC II variants probably causing megaloblastic anemias both correlated with unusual isoprotein patterns."}
{"id": "PMID:482909", "title": "[Cellular immunity: in vitro leukocyte migration inhibition does not correlate with the tuberculin skin test].", "content": "The leukocyte migration inhibition test (LMIT) is said to be an in-vitro correlate of cell mediated immunity in vivo. The authors' series of experiments, however, in which they compared skin tests and LMIT (taking the same tuberculin for both tests), showed wed no correlation between the two tests. The discrepancy between these results and those of other authors may be due to the fact that the authors worked under sterile conditions. It is known that contamination even by minute amounts of endotoxin may alter the results of in-vitro tests of cell mediated immunity. The authors therefore question the value of the LMIT as an in-vitro counterpart of the skin test.", "contents": "[Cellular immunity: in vitro leukocyte migration inhibition does not correlate with the tuberculin skin test]. The leukocyte migration inhibition test (LMIT) is said to be an in-vitro correlate of cell mediated immunity in vivo. The authors' series of experiments, however, in which they compared skin tests and LMIT (taking the same tuberculin for both tests), showed wed no correlation between the two tests. The discrepancy between these results and those of other authors may be due to the fact that the authors worked under sterile conditions. It is known that contamination even by minute amounts of endotoxin may alter the results of in-vitro tests of cell mediated immunity. The authors therefore question the value of the LMIT as an in-vitro counterpart of the skin test."}
{"id": "PMID:482910", "title": "[Sequential immunologic, cytochemical and autoradiographic studies on lymphoid cells in the blood of healthy adults].", "content": "Simultaneous characterization on a single slide of computer-mapped lymphoid blood cells by sequential analysis of 3 parameters (surface immunoglobulins, AET-SRBC-rosetting, unspecific acid alpha-naphthyl-acetate-esterase) served to distinguish 8 subpopulations. By previous in vitro incubation with 3HdT, the proliferative activity of the latter could also be assessed.", "contents": "[Sequential immunologic, cytochemical and autoradiographic studies on lymphoid cells in the blood of healthy adults]. Simultaneous characterization on a single slide of computer-mapped lymphoid blood cells by sequential analysis of 3 parameters (surface immunoglobulins, AET-SRBC-rosetting, unspecific acid alpha-naphthyl-acetate-esterase) served to distinguish 8 subpopulations. By previous in vitro incubation with 3HdT, the proliferative activity of the latter could also be assessed."}
{"id": "PMID:482911", "title": "[Diagnostic and therapeutic importance of splenectomy in patients with non-Hodgkin's lymphoma].", "content": "Whereas the contribution of exploratory laparotomy in Hodgkin's disease is well characterized, its value in Non-Hodgkin lymphoma (NHL) is not yet defined. This retrospective analysis of 31 cases is a contribution to the ongoing discussion. Laparotomy/splenectomy (LS) was done in 17 patients for diagnostic reasons and in 14 with therapeutic intent. Perioperative morbidity was low. In 17 cases the NHL had infiltrated the spleen. Indications for therapeutic LS were hemolytic anemia, pancytopenia and excessive lymphocytosis with granulocytopenia. The therapeutic benefit from splenectomy was satisfactory, especially in patients with well-differentiated lymphocytic leukemia of type CLL. In contrast, the diagnostic value of LS was minimal, except in patients with first diagnosis of NHL through LS. There was no change in tumor stage in any case. However, 4 false-negative findings contrast with the rapidly adverse course in these patients. Routine LS in patients with NHL does not appear to be justified, but has its value in NHL with primary abdominal localization. Therapeutic splenectomy is of benefit for the majority of patients, particularly those with CLL.", "contents": "[Diagnostic and therapeutic importance of splenectomy in patients with non-Hodgkin's lymphoma]. Whereas the contribution of exploratory laparotomy in Hodgkin's disease is well characterized, its value in Non-Hodgkin lymphoma (NHL) is not yet defined. This retrospective analysis of 31 cases is a contribution to the ongoing discussion. Laparotomy/splenectomy (LS) was done in 17 patients for diagnostic reasons and in 14 with therapeutic intent. Perioperative morbidity was low. In 17 cases the NHL had infiltrated the spleen. Indications for therapeutic LS were hemolytic anemia, pancytopenia and excessive lymphocytosis with granulocytopenia. The therapeutic benefit from splenectomy was satisfactory, especially in patients with well-differentiated lymphocytic leukemia of type CLL. In contrast, the diagnostic value of LS was minimal, except in patients with first diagnosis of NHL through LS. There was no change in tumor stage in any case. However, 4 false-negative findings contrast with the rapidly adverse course in these patients. Routine LS in patients with NHL does not appear to be justified, but has its value in NHL with primary abdominal localization. Therapeutic splenectomy is of benefit for the majority of patients, particularly those with CLL."}
{"id": "PMID:482912", "title": "[The fibrin and plasma-protein content of human thrombi].", "content": "The protein composition of cell-free thrombus contained in abdominal aneurysms of the aorta was investigated using PAGE and immunoreactivity after solubilization. 1. The dry weight was 18--29%, with minor differences between various locations. 2. 35--40% of the dry weight was extractable with NaCl/citrate and was identified as albumin (80%) and IgG (15%). Trace amounts of fibrinogen immunoreactive material were present. 3. The NaCl/citrate insoluble part was 80% hydrolyzable with plasmin. The predominant fragment was D-D dimer (from cross-linked fibrin). In addition, fragments Dand E were observed. Reduction of the NaCl/citrate insoluble part with 2-mercaptoethanol resulted in almost complete solubilization. PAGE analysis demonstrated alpha-polmers, gamma-gamma dimers and little free alpha-chains, indicating almost complete cross-linking of fibrin. Thus, the major protein were cross-linked fibrin, albumin and IgG.", "contents": "[The fibrin and plasma-protein content of human thrombi]. The protein composition of cell-free thrombus contained in abdominal aneurysms of the aorta was investigated using PAGE and immunoreactivity after solubilization. 1. The dry weight was 18--29%, with minor differences between various locations. 2. 35--40% of the dry weight was extractable with NaCl/citrate and was identified as albumin (80%) and IgG (15%). Trace amounts of fibrinogen immunoreactive material were present. 3. The NaCl/citrate insoluble part was 80% hydrolyzable with plasmin. The predominant fragment was D-D dimer (from cross-linked fibrin). In addition, fragments Dand E were observed. Reduction of the NaCl/citrate insoluble part with 2-mercaptoethanol resulted in almost complete solubilization. PAGE analysis demonstrated alpha-polmers, gamma-gamma dimers and little free alpha-chains, indicating almost complete cross-linking of fibrin. Thus, the major protein were cross-linked fibrin, albumin and IgG."}
{"id": "PMID:482913", "title": "[Importance of the sialic acid moiety for the heterogeneity in human fibrinogen].", "content": "To determine whether the observed heterogeneity of gamma- and B beta-polypeptide chains of human fibrinogen (2 and 1 sialic acid residue per chain) is due to differences in sialic acid content, fibrinogen was desialatgen was compared with normal fibrinogen. The gamma-chain heterogeneity observed in normal fibrinogen was absent in asialofibrinogen, whereas the B beta-chain heterogeneity appeared to be unaffected. Although the variants were indistinguishable on SDS-PAGE, isoelectric focusing in the presence of urea demonstrated heterogeneities of both gamma- and B beta-cahins even in asialofibrinogen. However, fewer bands were recognized in asialofibrinogen. The difference in sialic acid content of the gamma- and B beta-chain variants of human fibrinogen therefore explains on part of the polypeptide chain heterogeneity.", "contents": "[Importance of the sialic acid moiety for the heterogeneity in human fibrinogen]. To determine whether the observed heterogeneity of gamma- and B beta-polypeptide chains of human fibrinogen (2 and 1 sialic acid residue per chain) is due to differences in sialic acid content, fibrinogen was desialatgen was compared with normal fibrinogen. The gamma-chain heterogeneity observed in normal fibrinogen was absent in asialofibrinogen, whereas the B beta-chain heterogeneity appeared to be unaffected. Although the variants were indistinguishable on SDS-PAGE, isoelectric focusing in the presence of urea demonstrated heterogeneities of both gamma- and B beta-cahins even in asialofibrinogen. However, fewer bands were recognized in asialofibrinogen. The difference in sialic acid content of the gamma- and B beta-chain variants of human fibrinogen therefore explains on part of the polypeptide chain heterogeneity."}
{"id": "PMID:482916", "title": "[Pharmacologic remobilization of hyperadhesive granulocytes: a new principle in \"anti-inflammatory\" therapy].", "content": "It is thought that the nonsteroidal anti-inflammatory drugs act through inhibition of cyclooxygenase (CO). The authors show that the pyrazolon derivatives phenylbutazone (P) and sulfinpyrazone (S) affect PMN function in a manner independent of CO inhibition. During inflammation PMN often show increased adhesiveness. Such adhesiveness can be provoked in vitro by high concentrations of chemotaxins. Preincubation (10--20 minutes) of platelet-free human PMN suspensions in heat-inactivated plasma with 100 micrograms P or S per ml completely abolished a submaximal adherence induction on Petri dishes from 4% adherent cells in the absence, to 23% in the presence, of 10(-7) M of the chemotaxin N-f-Met-Leu-Phe (FP). In vivo, premedication of rabbits with P or S prevented the FP-induced neutropenia, e.g. 10 mg/kg of S blocked a 5-minutes agranulocytosis. P and S also abrogated adherence-induced lysosomal enzyme release and FP-stimulated hexose monophosphate pathway (HMP) activity. FP-induced hyperadhesiveness impedes PMN locomotion. Preincubation of PMN with P or S reestablished random motility and allowed chemotactic migration toward activated C (as C5a) in spite of the presence of 'adhesive' concentrations of FP. The potent CO inhibitors indomethacin and aspirin had no effect on FP-induced adherence, enzyme release, neutropenia and HMP stimulation. In 3 selected patients with PMN hyperadhesiveness, correction of this adhesiveness by P paralleled clinical remission. It is concluded that P and S exert their antiinflammatory action at least in part by interfering with PMN hyperadhesiveness and lysosomal enzyme release. These effects are independent of the prostaglandin-thromboxane system, since other CO inhibitors are uneffective.", "contents": "[Pharmacologic remobilization of hyperadhesive granulocytes: a new principle in \"anti-inflammatory\" therapy]. It is thought that the nonsteroidal anti-inflammatory drugs act through inhibition of cyclooxygenase (CO). The authors show that the pyrazolon derivatives phenylbutazone (P) and sulfinpyrazone (S) affect PMN function in a manner independent of CO inhibition. During inflammation PMN often show increased adhesiveness. Such adhesiveness can be provoked in vitro by high concentrations of chemotaxins. Preincubation (10--20 minutes) of platelet-free human PMN suspensions in heat-inactivated plasma with 100 micrograms P or S per ml completely abolished a submaximal adherence induction on Petri dishes from 4% adherent cells in the absence, to 23% in the presence, of 10(-7) M of the chemotaxin N-f-Met-Leu-Phe (FP). In vivo, premedication of rabbits with P or S prevented the FP-induced neutropenia, e.g. 10 mg/kg of S blocked a 5-minutes agranulocytosis. P and S also abrogated adherence-induced lysosomal enzyme release and FP-stimulated hexose monophosphate pathway (HMP) activity. FP-induced hyperadhesiveness impedes PMN locomotion. Preincubation of PMN with P or S reestablished random motility and allowed chemotactic migration toward activated C (as C5a) in spite of the presence of 'adhesive' concentrations of FP. The potent CO inhibitors indomethacin and aspirin had no effect on FP-induced adherence, enzyme release, neutropenia and HMP stimulation. In 3 selected patients with PMN hyperadhesiveness, correction of this adhesiveness by P paralleled clinical remission. It is concluded that P and S exert their antiinflammatory action at least in part by interfering with PMN hyperadhesiveness and lysosomal enzyme release. These effects are independent of the prostaglandin-thromboxane system, since other CO inhibitors are uneffective."}
{"id": "PMID:482917", "title": "[Sideroblastic anemia: clinical and hematological study on 57 patients].", "content": "Sideroblastic anemias (SA) are a heterogeneous group of hematologic disorders marked by a defect of heme synthesis, disturbance of iron metabolism and the presence of ringed sideroblasts. 57 cases of SA, including 23 primary and 34 secondary forms, are discussed. In the group of secondary SA 13 patients were alcoholics, 3 had received chloramphenicol, 2 had lead poisoning, 2 had received busulfan and 2 had immune hemolytic anemia. In some cases serum ferritin was found to be excessively high. The caryotype of one patient presented deletion of the long arm on chromosome 20. None of the patients with primary SA responded to vitamin treatment, and 3 developed a myeloproliferative syndrome.", "contents": "[Sideroblastic anemia: clinical and hematological study on 57 patients]. Sideroblastic anemias (SA) are a heterogeneous group of hematologic disorders marked by a defect of heme synthesis, disturbance of iron metabolism and the presence of ringed sideroblasts. 57 cases of SA, including 23 primary and 34 secondary forms, are discussed. In the group of secondary SA 13 patients were alcoholics, 3 had received chloramphenicol, 2 had lead poisoning, 2 had received busulfan and 2 had immune hemolytic anemia. In some cases serum ferritin was found to be excessively high. The caryotype of one patient presented deletion of the long arm on chromosome 20. None of the patients with primary SA responded to vitamin treatment, and 3 developed a myeloproliferative syndrome."}
{"id": "PMID:482918", "title": "[Similarity of the HL-A antigens in parents of patients with acute leukemia or aplastic anemia].", "content": "The frequency of common HLA-A and -B antigens was determined in 30 couples with a child suffering from acute leukemia (AL), 34 couples with a child suffering from aplastic anemia (AA) and 58 random couples with healthy children. Increased frequency of couples sharing at least two common antigens was observed in parents of both AL and AA children.", "contents": "[Similarity of the HL-A antigens in parents of patients with acute leukemia or aplastic anemia]. The frequency of common HLA-A and -B antigens was determined in 30 couples with a child suffering from acute leukemia (AL), 34 couples with a child suffering from aplastic anemia (AA) and 58 random couples with healthy children. Increased frequency of couples sharing at least two common antigens was observed in parents of both AL and AA children."}
{"id": "PMID:482925", "title": "Metabolism of americium-241 in man: an unusual case of internal contamination of a child and his father.", "content": "The metabolism of americium-241 has been studied during an 8-year period in an adult male and his son who, at the ages of 50 and 4 years, respectively, were accidentally and unknowingly contaminated within their home by means of inhalation. Chelation therapy with calcium trisodium pentetate was more effective in enhancing the removal of americium-241 from the child than from the father.", "contents": "Metabolism of americium-241 in man: an unusual case of internal contamination of a child and his father. The metabolism of americium-241 has been studied during an 8-year period in an adult male and his son who, at the ages of 50 and 4 years, respectively, were accidentally and unknowingly contaminated within their home by means of inhalation. Chelation therapy with calcium trisodium pentetate was more effective in enhancing the removal of americium-241 from the child than from the father."}
{"id": "PMID:482926", "title": "Neonatal rat surgery: avoiding maternal cannibalism.", "content": "A simple program of handling and care of pregnant rats before delivery makes it possible to carry out surgical procedures on newborn pups without resultant cannibalism or rejection of the operated animals by their mothers.", "contents": "Neonatal rat surgery: avoiding maternal cannibalism. A simple program of handling and care of pregnant rats before delivery makes it possible to carry out surgical procedures on newborn pups without resultant cannibalism or rejection of the operated animals by their mothers."}
{"id": "PMID:482927", "title": "Cerebral norepinephrine: influence on cortical oxidative metabolism in situ.", "content": "Unilateral lesion of the locus coeruleus and the resultant norepinephrine depletion in the ipsilateral cerebrum alters the relationship between cerebral metabolic demands and local delivery of oxygen and substrates. This effect of norepinephrine depletion is demonstrated by slower recovery of the redox ratio of cytochrome a,a3 during increased metabolic demands induced by local cortical stimulation.", "contents": "Cerebral norepinephrine: influence on cortical oxidative metabolism in situ. Unilateral lesion of the locus coeruleus and the resultant norepinephrine depletion in the ipsilateral cerebrum alters the relationship between cerebral metabolic demands and local delivery of oxygen and substrates. This effect of norepinephrine depletion is demonstrated by slower recovery of the redox ratio of cytochrome a,a3 during increased metabolic demands induced by local cortical stimulation."}
{"id": "PMID:482928", "title": "Shark heart mitochondria: effects of external osmolality on respiration.", "content": "Shark mitochondrial respiration was studied in media with osmolalities between 160 and 1500 milliosmoles. The respiratory control ratio, a marker for functional integrity of the isolated mitochondria, was maximal at 1000 millismoles and decreased during hypotonic or hypertonic exposure. Shark mitochondria function best at their native tonicity, a value that produces abnormal function in mammalian mitochondria.", "contents": "Shark heart mitochondria: effects of external osmolality on respiration. Shark mitochondrial respiration was studied in media with osmolalities between 160 and 1500 milliosmoles. The respiratory control ratio, a marker for functional integrity of the isolated mitochondria, was maximal at 1000 millismoles and decreased during hypotonic or hypertonic exposure. Shark mitochondria function best at their native tonicity, a value that produces abnormal function in mammalian mitochondria."}
{"id": "PMID:482929", "title": "Dopamine auto- and postsynaptic receptors: electrophysiological evidence for differential sensitivity to dopamine agonists.", "content": "The responses of dopamine cells in the substantia nigra to iontophoretically administered dopamine and intravenous apomorphine were compared to the responses of spontaneously active neurons in the caudate nucleus. Dopaminergic cells were six to ten times more sensitive to dopamine and intravenous apomorphine than 86 percent of the caudate cells tested. This differential sensitivity of dopamine auto- and postsynaptic receptors may explain the apparently paradoxical behavioral effects induced by small compared to large doses of some dopamine agonists and may provide a means of developing new types of drugs to antagonize dopaminergic influence in the central nervous system.", "contents": "Dopamine auto- and postsynaptic receptors: electrophysiological evidence for differential sensitivity to dopamine agonists. The responses of dopamine cells in the substantia nigra to iontophoretically administered dopamine and intravenous apomorphine were compared to the responses of spontaneously active neurons in the caudate nucleus. Dopaminergic cells were six to ten times more sensitive to dopamine and intravenous apomorphine than 86 percent of the caudate cells tested. This differential sensitivity of dopamine auto- and postsynaptic receptors may explain the apparently paradoxical behavioral effects induced by small compared to large doses of some dopamine agonists and may provide a means of developing new types of drugs to antagonize dopaminergic influence in the central nervous system."}
{"id": "PMID:482930", "title": "Serotonin neurons project to small blood vessels in the brain.", "content": "Electrolytic lesions of the nucleus raphe dorsalis and medianus reduce the concentration of serotonin (5-hydroxytryptamine) within rat brain intraparenchymal blood vessels. The concentration of serotonin within these vessels increases or decreases after the administration of drugs that modify the biosynthesis and degradation of serotonin or destroy nerve terminals by an uptake-dependent mechanism. These studies provide evidence for the existence of a serotonin-containing pathway seemingly analogous to the neuronal projection that terminates on small parenchymal blood vessels from noradrenergic neurons of the locus coeruleus.", "contents": "Serotonin neurons project to small blood vessels in the brain. Electrolytic lesions of the nucleus raphe dorsalis and medianus reduce the concentration of serotonin (5-hydroxytryptamine) within rat brain intraparenchymal blood vessels. The concentration of serotonin within these vessels increases or decreases after the administration of drugs that modify the biosynthesis and degradation of serotonin or destroy nerve terminals by an uptake-dependent mechanism. These studies provide evidence for the existence of a serotonin-containing pathway seemingly analogous to the neuronal projection that terminates on small parenchymal blood vessels from noradrenergic neurons of the locus coeruleus."}
{"id": "PMID:482931", "title": "Correct axonal regeneration after target cell removal in the central nervous system of the leech.", "content": "The unique target neuron of a severed axon in the leech was selectively eliminated by intracellular injection of protease. In the absence of the target, the severed axon regenerated normally along its original pathway to the usual site of synapse, where it stopped growing without forming alternative connections.", "contents": "Correct axonal regeneration after target cell removal in the central nervous system of the leech. The unique target neuron of a severed axon in the leech was selectively eliminated by intracellular injection of protease. In the absence of the target, the severed axon regenerated normally along its original pathway to the usual site of synapse, where it stopped growing without forming alternative connections."}
{"id": "PMID:482937", "title": "Calcification of differentiating skeletal mesenchyme in vitro.", "content": "Embryonic limb-bud mesenchyme was induced to calcify in culture by the addition of 3 mM inorganic phosphate to the medium. Phosphate enhanced calcification of the matrix produced by mesenchymal or fibroblast-like cells, whereas no calcification was evident in areas where cartilage had developed. However, calcification was induced throughout the cell layer by altering the cartilage matrix properties with certain enzymes or by changing the phenotypic expression of the cells with vitamin A.", "contents": "Calcification of differentiating skeletal mesenchyme in vitro. Embryonic limb-bud mesenchyme was induced to calcify in culture by the addition of 3 mM inorganic phosphate to the medium. Phosphate enhanced calcification of the matrix produced by mesenchymal or fibroblast-like cells, whereas no calcification was evident in areas where cartilage had developed. However, calcification was induced throughout the cell layer by altering the cartilage matrix properties with certain enzymes or by changing the phenotypic expression of the cells with vitamin A."}
{"id": "PMID:482938", "title": "Exercise during development induces an increase in Purkinje cell dendritic tree size.", "content": "Mice allowed to exercise during the late postnatal period had Purkinje cells with larger dendritic trees and greater numbers of spines than littermates whose physical activity was severly restricted. These changes in Purkinje cells were accompanied by a selective reduction in the thickness of the cerebellar molecular layer. The data provide evidence for cerebellar plasticity during late development and demonstrate that physical activity can modify the development of Purkinje cell dendrites.", "contents": "Exercise during development induces an increase in Purkinje cell dendritic tree size. Mice allowed to exercise during the late postnatal period had Purkinje cells with larger dendritic trees and greater numbers of spines than littermates whose physical activity was severly restricted. These changes in Purkinje cells were accompanied by a selective reduction in the thickness of the cerebellar molecular layer. The data provide evidence for cerebellar plasticity during late development and demonstrate that physical activity can modify the development of Purkinje cell dendrites."}
{"id": "PMID:482939", "title": "Holographic assessment of a hypothesized microwave hearing mechanism.", "content": "Exposure of the head to pulse-modulated microwaves induces the perception of a sound. It has been hypothesized that the electromagnetic energy is converted to acoustic energy in the skull and then conducted through the bone. Dynamic time-averaged interferometric holography showed that the predicted motion of head tissue did not occur. An alternative locus for this hearing effect is suggested.", "contents": "Holographic assessment of a hypothesized microwave hearing mechanism. Exposure of the head to pulse-modulated microwaves induces the perception of a sound. It has been hypothesized that the electromagnetic energy is converted to acoustic energy in the skull and then conducted through the bone. Dynamic time-averaged interferometric holography showed that the predicted motion of head tissue did not occur. An alternative locus for this hearing effect is suggested."}
{"id": "PMID:482942", "title": "Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence.", "content": "The nucleotide sequence of a cloned rabbit chromosomal DNA segment of 1620 nucleotides length which contains a beta-globin gene is presented. The coding regions are separated into three blocks by two intervening sequences of 126 and 573 base pairs, respectively. The rabbit sequence was compared with a homologous mouse sequence. The segments flanking the rabbit gene, as well as the coding regions, the 5' noncoding and part of the 3' noncoding messenger RNA sequences are similar to those of the mouse gene; the homologous introns, despite identical location, are distinctly dissimilar except for the junction regions. Homologous introns may be derived from common ancestral introns by large insertions and deletions rather than be multiple point mutations.", "contents": "Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence. The nucleotide sequence of a cloned rabbit chromosomal DNA segment of 1620 nucleotides length which contains a beta-globin gene is presented. The coding regions are separated into three blocks by two intervening sequences of 126 and 573 base pairs, respectively. The rabbit sequence was compared with a homologous mouse sequence. The segments flanking the rabbit gene, as well as the coding regions, the 5' noncoding and part of the 3' noncoding messenger RNA sequences are similar to those of the mouse gene; the homologous introns, despite identical location, are distinctly dissimilar except for the junction regions. Homologous introns may be derived from common ancestral introns by large insertions and deletions rather than be multiple point mutations."}
{"id": "PMID:482943", "title": "Synaptic regeneration in identified neurons of the lamprey spinal cords.", "content": "Identified reticulospinal neurons whose giant axons were severed after spinal cord transection were filled with horseradish peroxidase. Whole mounts and serial-section light and electron micrographs show axon regeneration across the spinal lesion and the formation of new synapses. Normal swimming activity returns in the spinally transected animals, although the regenerated synapses are in atypical regions of the spinal cord.", "contents": "Synaptic regeneration in identified neurons of the lamprey spinal cords. Identified reticulospinal neurons whose giant axons were severed after spinal cord transection were filled with horseradish peroxidase. Whole mounts and serial-section light and electron micrographs show axon regeneration across the spinal lesion and the formation of new synapses. Normal swimming activity returns in the spinally transected animals, although the regenerated synapses are in atypical regions of the spinal cord."}
{"id": "PMID:482944", "title": "Neural axis representing target range in the auditory cortex of the mustache bat.", "content": "In echolocating bats, the primary cue for determining distance to a target is the interval between an emitted orientation sound and its echo. Whereas frequency is represented by place in the bat cochlea, no anatomical location represents of primary range. Target range is coded by the time interval between grouped discharges of primary auditory neurons in response to both the emitted sound and its echo. In the frequency-modulated-signal processing area of the auditory cortex of the mustache bat (Pteronotus parnellii rubiginosus), neurons respond poorly or not at all to synthesized orientation sounds or echoes alone but respond vigorously to echoes following the emitted sound with a specific delay from targets at a specific range. These range-tuned neurons are systemically arranged along the rostrocaudal axis of the frequency-modulated-signal processing area according to the delays to which they best respond, and thus represent target range in terms of cortical organization. The frequency-modulated-signal processing area therefore shows odotopic representation.", "contents": "Neural axis representing target range in the auditory cortex of the mustache bat. In echolocating bats, the primary cue for determining distance to a target is the interval between an emitted orientation sound and its echo. Whereas frequency is represented by place in the bat cochlea, no anatomical location represents of primary range. Target range is coded by the time interval between grouped discharges of primary auditory neurons in response to both the emitted sound and its echo. In the frequency-modulated-signal processing area of the auditory cortex of the mustache bat (Pteronotus parnellii rubiginosus), neurons respond poorly or not at all to synthesized orientation sounds or echoes alone but respond vigorously to echoes following the emitted sound with a specific delay from targets at a specific range. These range-tuned neurons are systemically arranged along the rostrocaudal axis of the frequency-modulated-signal processing area according to the delays to which they best respond, and thus represent target range in terms of cortical organization. The frequency-modulated-signal processing area therefore shows odotopic representation."}
{"id": "PMID:482945", "title": "Hydrostatic pressure reversibly blocks membrane control of ciliary motility in Paramecium.", "content": "A hydrostatic pressure of only 68 atmospheres prevented swimming Paramecium caudatum from \"avoiding\" or reversing direction; 170 atmospheres stopped or decreased forward velocity by more than 75 percent. A decompression of 40 atmospheres invoked a single reversal, even at ,80 atmospheres. In contrast, 170 atmospheres did not significantly affect swimming behavior of paramecium \"models\" that were reactivated in a solution containing adenosine triphosphate and magnesium ions after their membrane had been disrupted by Triton X-100.", "contents": "Hydrostatic pressure reversibly blocks membrane control of ciliary motility in Paramecium. A hydrostatic pressure of only 68 atmospheres prevented swimming Paramecium caudatum from \"avoiding\" or reversing direction; 170 atmospheres stopped or decreased forward velocity by more than 75 percent. A decompression of 40 atmospheres invoked a single reversal, even at ,80 atmospheres. In contrast, 170 atmospheres did not significantly affect swimming behavior of paramecium \"models\" that were reactivated in a solution containing adenosine triphosphate and magnesium ions after their membrane had been disrupted by Triton X-100."}
{"id": "PMID:482946", "title": "Light and efferent activity control rhabdom turnover in Limulus photoreceptors.", "content": "Photosensitive membrane structures in the retinular cells of the Limulus lateral eye are broken down and renewed daily. The first light onset causes a rapid, synchronous disassembly and buildup of the rhabdom in each photoreceptor cell. The entire process is complete within 30 minutes. Blocking the efferent input to the retina from the brain blocks the turnover of the rhabdom, and mimicking the efferent input by optic nerve stimulation restores it.", "contents": "Light and efferent activity control rhabdom turnover in Limulus photoreceptors. Photosensitive membrane structures in the retinular cells of the Limulus lateral eye are broken down and renewed daily. The first light onset causes a rapid, synchronous disassembly and buildup of the rhabdom in each photoreceptor cell. The entire process is complete within 30 minutes. Blocking the efferent input to the retina from the brain blocks the turnover of the rhabdom, and mimicking the efferent input by optic nerve stimulation restores it."}
{"id": "PMID:482947", "title": "Radionuclide therapy of hematologic disorders.", "content": "32P is effective therapy for polycythemia and primary thrombocytosis. The Polycythemia Vera Study Group is comparing radioactive phosphorus with alkylating agents to determine relative efficacy. Less well investigated is the effectiveness of 32P vs. busulfan in chronic granulocytic leukemia. Endolymphatic administration of radiopharmaceuticals may play a role in the therapy of infradiaphragmatic lymphoma. Among the radionuclides that have at times been used in hematology are 32P, 198Au 24Na, 76As, 89Sr, 52Mb, 54Mn, 91Y, 95Zr, 95Cb, 111Ag, 109Pd, 131I, 185W, and 192Ir. As stated, 32P has proven single most efficacious agent. The hematologic diseases that have been treated include both malignant and benign conditions. Among the malignant conditions are polycythemia vera, agnogenic myeloid metaplasia, thrombocythemia, leukemia, Hodgkin's disease, and multiple myeloma. Hemophilia, and Osler--Weber--Rendu disease are among the benign entities in which the agents have been tried. Polycythemia and thrombocythemia remain those in which the greatest success has been achieved.", "contents": "Radionuclide therapy of hematologic disorders. 32P is effective therapy for polycythemia and primary thrombocytosis. The Polycythemia Vera Study Group is comparing radioactive phosphorus with alkylating agents to determine relative efficacy. Less well investigated is the effectiveness of 32P vs. busulfan in chronic granulocytic leukemia. Endolymphatic administration of radiopharmaceuticals may play a role in the therapy of infradiaphragmatic lymphoma. Among the radionuclides that have at times been used in hematology are 32P, 198Au 24Na, 76As, 89Sr, 52Mb, 54Mn, 91Y, 95Zr, 95Cb, 111Ag, 109Pd, 131I, 185W, and 192Ir. As stated, 32P has proven single most efficacious agent. The hematologic diseases that have been treated include both malignant and benign conditions. Among the malignant conditions are polycythemia vera, agnogenic myeloid metaplasia, thrombocythemia, leukemia, Hodgkin's disease, and multiple myeloma. Hemophilia, and Osler--Weber--Rendu disease are among the benign entities in which the agents have been tried. Polycythemia and thrombocythemia remain those in which the greatest success has been achieved."}
{"id": "PMID:482949", "title": "Iatrogenic alterations in radionuclide biodistributions.", "content": "The biodistribution of radiotracers used in diagnostic imaging is grossly and recognizably altered by a wide variety of drugs and other treatment modalities, such as surgery and radiotherapy. Knowledge of such altered biodistribution is important both in making diagnostic inferences from scans and in dosimetric considerations.", "contents": "Iatrogenic alterations in radionuclide biodistributions. The biodistribution of radiotracers used in diagnostic imaging is grossly and recognizably altered by a wide variety of drugs and other treatment modalities, such as surgery and radiotherapy. Knowledge of such altered biodistribution is important both in making diagnostic inferences from scans and in dosimetric considerations."}
{"id": "PMID:482948", "title": "Potential future application with therapeutic agents.", "content": "Several new approaches to radiation therapy with radionuclides have been discussed. Iron 55 is selectively utilized in the red cell developmental cycle and in therapeutic doses, can lower marrow and circulating erythrocyte levels with much smaller degrees of effect on other cell lines. A serious complication, noted in animal studies, is the induction of neoplasma, especially osteosarcoma. Selective irradiation of the cell nucleus is possible with 125IUdR. This results in highly efficient cell killing due to the highly concentrated region of ionization. High concentrations of densely ionizing radiation in the malignant cell may also be accomplished with 211At. The use of labeled liposomes is an additional approach to the delivery of intracellular irradiation. None of these approaches is applicable for the practical treatment of human malignancy at the present time. The importance of these approaches is their value as models for future development of methods that can provide highly selective radiation to target sites.", "contents": "Potential future application with therapeutic agents. Several new approaches to radiation therapy with radionuclides have been discussed. Iron 55 is selectively utilized in the red cell developmental cycle and in therapeutic doses, can lower marrow and circulating erythrocyte levels with much smaller degrees of effect on other cell lines. A serious complication, noted in animal studies, is the induction of neoplasma, especially osteosarcoma. Selective irradiation of the cell nucleus is possible with 125IUdR. This results in highly efficient cell killing due to the highly concentrated region of ionization. High concentrations of densely ionizing radiation in the malignant cell may also be accomplished with 211At. The use of labeled liposomes is an additional approach to the delivery of intracellular irradiation. None of these approaches is applicable for the practical treatment of human malignancy at the present time. The importance of these approaches is their value as models for future development of methods that can provide highly selective radiation to target sites."}
{"id": "PMID:482953", "title": "Radiotherapeutic agents: properties, dosimetry, and radiobiologic considerations.", "content": "Radioactive nuclides for treatment have occupied an important but somewhat diminishing role in the total practice of nuclear medicine. Although theoretically they should have important potentialities, particularly in the treatment of various forms of cancer, their development in this field has not kept pace with the progress in other treatment modalities in radiation oncology. Indications for the selection of appropriate isotopes for therapy revolve about the emission of beta particles of sufficient energy, which are administered in a chemical form that reaches the tumor. Methods of calculation of doses delivered to sites of deposition are discussed in the text. Radiobiologic considerations include the possibility of early deleterious effects from overdosage, and consideration of chromosomal changes of circulating lymphocytes and their implications. Late effects that have been of great public concern are confined almost solely to possible carcinogenesis, and this effect has been minimal in patients receiving therapeutic levels of radioactive drugs. Genetic and developmental effects, also, have been negligible. Complications encountered more frequently have been leukemia after extensive therapy of thyroid carcinoma, and local fibrosis after direct injection of radioactive colloids into tumor tissue.", "contents": "Radiotherapeutic agents: properties, dosimetry, and radiobiologic considerations. Radioactive nuclides for treatment have occupied an important but somewhat diminishing role in the total practice of nuclear medicine. Although theoretically they should have important potentialities, particularly in the treatment of various forms of cancer, their development in this field has not kept pace with the progress in other treatment modalities in radiation oncology. Indications for the selection of appropriate isotopes for therapy revolve about the emission of beta particles of sufficient energy, which are administered in a chemical form that reaches the tumor. Methods of calculation of doses delivered to sites of deposition are discussed in the text. Radiobiologic considerations include the possibility of early deleterious effects from overdosage, and consideration of chromosomal changes of circulating lymphocytes and their implications. Late effects that have been of great public concern are confined almost solely to possible carcinogenesis, and this effect has been minimal in patients receiving therapeutic levels of radioactive drugs. Genetic and developmental effects, also, have been negligible. Complications encountered more frequently have been leukemia after extensive therapy of thyroid carcinoma, and local fibrosis after direct injection of radioactive colloids into tumor tissue."}
{"id": "PMID:482954", "title": "The treatment of benign thyroid disease.", "content": "The treatment of benign forms of thyroid disease is reviewed. Endemic goiter is a public health problem preventable by the addition of iodine to the food or water supply. Endemic and familial goiters are treated with replacement doses of I-thyroxine, as are sporadic colloid goiters and goiters resulting from chronic thyroiditis. Hyperfunctioning autionomous nodules without thyrotoxicosis and cystic nodules require no specific therapy. Prophylaxis against diffuse or nodular goiter after radiation to the head or neck for therapeutic purposes with thyroxine replacement therapy is debatable. All forms of hypothyroidism, including incipient types, require replacement thyroxine therapy, but this should be undertaken cautiously in older patients and in those with evidence of ischemic myocardial disease. Myxedema coma requires vigorous treatment and detailed supervision because of dismal mortality rates. Iodine 131 is the treatment of choice in diffuse toxic goiter, but alternative forms.", "contents": "The treatment of benign thyroid disease. The treatment of benign forms of thyroid disease is reviewed. Endemic goiter is a public health problem preventable by the addition of iodine to the food or water supply. Endemic and familial goiters are treated with replacement doses of I-thyroxine, as are sporadic colloid goiters and goiters resulting from chronic thyroiditis. Hyperfunctioning autionomous nodules without thyrotoxicosis and cystic nodules require no specific therapy. Prophylaxis against diffuse or nodular goiter after radiation to the head or neck for therapeutic purposes with thyroxine replacement therapy is debatable. All forms of hypothyroidism, including incipient types, require replacement thyroxine therapy, but this should be undertaken cautiously in older patients and in those with evidence of ischemic myocardial disease. Myxedema coma requires vigorous treatment and detailed supervision because of dismal mortality rates. Iodine 131 is the treatment of choice in diffuse toxic goiter, but alternative forms."}
{"id": "PMID:482955", "title": "Treatment of malignant thyroid disease.", "content": "Surgery is the primary form of therapy in the management of malignant thyroid disease. A near-total thyroidectomy is the preferred approach. Radioactive iodine is used for supplementary ablation therapy and for definitive therapy in differentiated tumors of papillary or follicular cell type. Thyroid-stimulating hormone (TSH) is administered in conjunction with radioactive iodine therapy, since tumor uptake appears to be directly related to endogenous TSH levels. Therapeutic doses of radioactive iodine range from 100 to 200 mCi of 131 I depending on tumor distribution. Adequate thyroid hormone replacement therapy resulting in the suppression of TSH is of considerable value in the prevention of tumor recurrence. Due to the possibility of late recurrence, patients should be followed for indefinite periods by means of diagnostic imaging studies at 1-2 yr intervals. Despite 30 yr of experience, the therapeutic efficacy of radioactive iodine remains controversial. However, in recent years, there has been mounting evidence indicating increased survival and decreased tumor recurrence in radioactive iodine-treated patients. External radiation therapy is reserved for anaplastic carcinoma and lymphoma, and adenocarcinomas that are refractory to radioactive iodine. Chemotherapy experience is limited; however, some reduction in the size of metastatic lesions has been observed after the administration of adriamycin.", "contents": "Treatment of malignant thyroid disease. Surgery is the primary form of therapy in the management of malignant thyroid disease. A near-total thyroidectomy is the preferred approach. Radioactive iodine is used for supplementary ablation therapy and for definitive therapy in differentiated tumors of papillary or follicular cell type. Thyroid-stimulating hormone (TSH) is administered in conjunction with radioactive iodine therapy, since tumor uptake appears to be directly related to endogenous TSH levels. Therapeutic doses of radioactive iodine range from 100 to 200 mCi of 131 I depending on tumor distribution. Adequate thyroid hormone replacement therapy resulting in the suppression of TSH is of considerable value in the prevention of tumor recurrence. Due to the possibility of late recurrence, patients should be followed for indefinite periods by means of diagnostic imaging studies at 1-2 yr intervals. Despite 30 yr of experience, the therapeutic efficacy of radioactive iodine remains controversial. However, in recent years, there has been mounting evidence indicating increased survival and decreased tumor recurrence in radioactive iodine-treated patients. External radiation therapy is reserved for anaplastic carcinoma and lymphoma, and adenocarcinomas that are refractory to radioactive iodine. Chemotherapy experience is limited; however, some reduction in the size of metastatic lesions has been observed after the administration of adriamycin."}
{"id": "PMID:482974", "title": "Comparison of media for dissemination of information about cancer in rural communities.", "content": "A study was done in Orangeburg County, South Carolina, to compare the effectiveness of the various mass media--television, radio, and newspaper--for the dissemination of educational material about cancer. Effectiveness of these media in reaching certain subpopulations defined by race, sex, income, and population density was also examined. The newspaper was found to be generally more effective than other media. This superiority held for almost all subpopulations. Furthermore, it was found that certain subpopulations differed from others in the extent to which they were reached by a particular medium.", "contents": "Comparison of media for dissemination of information about cancer in rural communities. A study was done in Orangeburg County, South Carolina, to compare the effectiveness of the various mass media--television, radio, and newspaper--for the dissemination of educational material about cancer. Effectiveness of these media in reaching certain subpopulations defined by race, sex, income, and population density was also examined. The newspaper was found to be generally more effective than other media. This superiority held for almost all subpopulations. Furthermore, it was found that certain subpopulations differed from others in the extent to which they were reached by a particular medium."}
{"id": "PMID:482975", "title": "Caustic burns of the esophagus: ten-year results of aggressive care.", "content": "In 1967, care of esophageal burns from caustic substances was altered to include immediate esophagoscopy, high doses of steroid, and follow-up dilatations plus cine-esophagograms as needed. Previously, this area averaged six repairs of esophageal stricutre each year. Since 1967, there have been no esophageal strictures in 100 patients.", "contents": "Caustic burns of the esophagus: ten-year results of aggressive care. In 1967, care of esophageal burns from caustic substances was altered to include immediate esophagoscopy, high doses of steroid, and follow-up dilatations plus cine-esophagograms as needed. Previously, this area averaged six repairs of esophageal stricutre each year. Since 1967, there have been no esophageal strictures in 100 patients."}
{"id": "PMID:482976", "title": "Traumatic rupture of the thoracic aorta.", "content": "Aortic transection secondary to blunt trauma is not an uncommon injury. Prompt recognition and treatment of the injury is required to salvage patients with this type of injury. Ten patients with transection of the thoracic aorta at the isthmus have been treated in the past two years at our institution. There have been two operative deaths, one delayed hospital death, and seven survivors. We believe the use of a temporary shunt of heparin-bonded polyvinylchloride during the repair of the aorta offers significant advantages over other methods which have been described.", "contents": "Traumatic rupture of the thoracic aorta. Aortic transection secondary to blunt trauma is not an uncommon injury. Prompt recognition and treatment of the injury is required to salvage patients with this type of injury. Ten patients with transection of the thoracic aorta at the isthmus have been treated in the past two years at our institution. There have been two operative deaths, one delayed hospital death, and seven survivors. We believe the use of a temporary shunt of heparin-bonded polyvinylchloride during the repair of the aorta offers significant advantages over other methods which have been described."}
{"id": "PMID:482977", "title": "Treatment of metabolic alkalosis with intravenous hydrochloric acid.", "content": "Severe alkalosis requires aggressive treatment. Twenty patients at the Talmadge Memorial Hospital have been treated for metabolic alkalosis by infusion of dilute hydrochloric acid through a central venous line. The treatment was effective and there were no major complications. Intravenous hydrochloric acid is preferred to other modes of therapy for refractory alkalosis, especially in the presence of hepatic or renal failure.", "contents": "Treatment of metabolic alkalosis with intravenous hydrochloric acid. Severe alkalosis requires aggressive treatment. Twenty patients at the Talmadge Memorial Hospital have been treated for metabolic alkalosis by infusion of dilute hydrochloric acid through a central venous line. The treatment was effective and there were no major complications. Intravenous hydrochloric acid is preferred to other modes of therapy for refractory alkalosis, especially in the presence of hepatic or renal failure."}
{"id": "PMID:482978", "title": "Effect of lidocaine anesthesia on pattern of ventilation and pulmonary function tests.", "content": "The pattern of ventilation (consisting of tidal volume, frequency of breathing, minute ventilation, peak inspiratory flow rate, inspiratory time, mean inspiratory flow rate, and inspiatory time ratio) and pulmonary function tests (consisting of spirometry, lung volumes, and airway resistance) were measured in ten conscious, healthy subjects before and after anesthesia of the airways achieved by inhalation of lidocaine, to determine the role of the vagus nerves in the control of eupneic breathing. No significant change was found in the pattern of ventilation or in pulmonary function tests after airway anesthesia. These results are consistent with the observation that the vagus nerves do not play an important role in the control of eupneic breathing in humans.", "contents": "Effect of lidocaine anesthesia on pattern of ventilation and pulmonary function tests. The pattern of ventilation (consisting of tidal volume, frequency of breathing, minute ventilation, peak inspiratory flow rate, inspiratory time, mean inspiratory flow rate, and inspiatory time ratio) and pulmonary function tests (consisting of spirometry, lung volumes, and airway resistance) were measured in ten conscious, healthy subjects before and after anesthesia of the airways achieved by inhalation of lidocaine, to determine the role of the vagus nerves in the control of eupneic breathing. No significant change was found in the pattern of ventilation or in pulmonary function tests after airway anesthesia. These results are consistent with the observation that the vagus nerves do not play an important role in the control of eupneic breathing in humans."}
{"id": "PMID:482979", "title": "Safety and efficacy of micronized estradiol vaginal cream.", "content": "Fifty-four postmenopausal patients with atrophic vaginitis satisfactorily completed one to two months' therapy with daily intravaginal application of 0.2 mg of 17 beta-estradiol in a cream base. No significant alterations were noted in the hematologic biochemical, and urine analyses. A number of response criteria showed significant improvement (P less than .01), including the severity rating for atrophic vaginitis, the maturation index of vaginal cells, the frequency and severity of hot flashes, the presence of vaginal dryness and itching, dyspareunia, skin flushing, and the severity of sweating episodes. The incidence of side effects was low and included breast tenderness and abdominal cramping.", "contents": "Safety and efficacy of micronized estradiol vaginal cream. Fifty-four postmenopausal patients with atrophic vaginitis satisfactorily completed one to two months' therapy with daily intravaginal application of 0.2 mg of 17 beta-estradiol in a cream base. No significant alterations were noted in the hematologic biochemical, and urine analyses. A number of response criteria showed significant improvement (P less than .01), including the severity rating for atrophic vaginitis, the maturation index of vaginal cells, the frequency and severity of hot flashes, the presence of vaginal dryness and itching, dyspareunia, skin flushing, and the severity of sweating episodes. The incidence of side effects was low and included breast tenderness and abdominal cramping."}
{"id": "PMID:482980", "title": "Major venous anomalies complicating retroperitoneal surgery.", "content": "Transposition of the inferior vena cava, duplication of the inferior vena cava, circumaortic renal collar, and retroaortic renal vein constitute the four most common major venous anomalies occurring in the retroperitoneum. Recent clinical cases are presented to review the embryogenesis, incidence, and clinical importance of these anomalies and to alert the unfamiliar surgeon to their possible existence. A high index of suspicion on the part of the surgeon is required to prevent inadvertent injury to these anomalous veins and to avoid significant hemorrhage during retroperitoneal surgery.", "contents": "Major venous anomalies complicating retroperitoneal surgery. Transposition of the inferior vena cava, duplication of the inferior vena cava, circumaortic renal collar, and retroaortic renal vein constitute the four most common major venous anomalies occurring in the retroperitoneum. Recent clinical cases are presented to review the embryogenesis, incidence, and clinical importance of these anomalies and to alert the unfamiliar surgeon to their possible existence. A high index of suspicion on the part of the surgeon is required to prevent inadvertent injury to these anomalous veins and to avoid significant hemorrhage during retroperitoneal surgery."}
{"id": "PMID:482981", "title": "Amenorrhea secondary to voluntary weight loss.", "content": "Obese patients who voluntarily reduce to a normal weight may develop secondary amenorrhea. Six young women who dieted to lose from 13 to 50 pounds, including four from an obese weight, were evaluated because of absent cervical mucus ferning, hypoestrogenic vaginal smears, and failure to have withdrawal menses from a progestogen. Serum FSH values were normal in all, while four had normal serum LH and two had low serum LH levels. T4 and/or T3 uptake was normal in all. The pituitary-adrenal axis was apparently intact since baseline urinary steroids were normal as was the response to both ACTH and metyrapone. Fasting serum growth hormone was markedly elevated in two and slightly elevated in three, with the other patinet demonstrating an unusually high response to glucagon/propranolol in the 30 minute specimen. These endocrine findings are similar to those observed in patients with anorexia nervosa, but the weight loss is entirely voluntary and there was no associated psychiatric abnormality.", "contents": "Amenorrhea secondary to voluntary weight loss. Obese patients who voluntarily reduce to a normal weight may develop secondary amenorrhea. Six young women who dieted to lose from 13 to 50 pounds, including four from an obese weight, were evaluated because of absent cervical mucus ferning, hypoestrogenic vaginal smears, and failure to have withdrawal menses from a progestogen. Serum FSH values were normal in all, while four had normal serum LH and two had low serum LH levels. T4 and/or T3 uptake was normal in all. The pituitary-adrenal axis was apparently intact since baseline urinary steroids were normal as was the response to both ACTH and metyrapone. Fasting serum growth hormone was markedly elevated in two and slightly elevated in three, with the other patinet demonstrating an unusually high response to glucagon/propranolol in the 30 minute specimen. These endocrine findings are similar to those observed in patients with anorexia nervosa, but the weight loss is entirely voluntary and there was no associated psychiatric abnormality."}
{"id": "PMID:482982", "title": "Incidence of sepsis in neonates with clinical respiratory distress.", "content": "Respiratory distress is the most frequent symptom initiating referral to neonatal intensive care centers. Previous reports have indicated that 40% of infants with sepsis proved by blood culture had respiratory symptoms as their clinical presentation. In this prospective study there were 145 consecutive infants referred because of respiratory distress. Two-site blood cultures were collated with all other clinical and laboratory data to confirm a diagnosis of septicemia. There was a 4.8% incidence of bacteremia, and clinical and laboratory parameters confirmed septicemia in 3.5% of infants studied. Sixty percent of these infants had group B streptococcal sepsis, and 80% had leukopenia. The low incidence of confirmed septicemia in infants referred because of respiratory distress suggests that antibiotic therapy should not be routine.", "contents": "Incidence of sepsis in neonates with clinical respiratory distress. Respiratory distress is the most frequent symptom initiating referral to neonatal intensive care centers. Previous reports have indicated that 40% of infants with sepsis proved by blood culture had respiratory symptoms as their clinical presentation. In this prospective study there were 145 consecutive infants referred because of respiratory distress. Two-site blood cultures were collated with all other clinical and laboratory data to confirm a diagnosis of septicemia. There was a 4.8% incidence of bacteremia, and clinical and laboratory parameters confirmed septicemia in 3.5% of infants studied. Sixty percent of these infants had group B streptococcal sepsis, and 80% had leukopenia. The low incidence of confirmed septicemia in infants referred because of respiratory distress suggests that antibiotic therapy should not be routine."}
{"id": "PMID:482983", "title": "Use of transverse Kirschner wires in comminuted facial fractures.", "content": "Although most facial fractures can be well managed with open reduction and direct wiring of the fracture sites, this treatment is inadequate for some comminuted fractures and further fixation is required. At Charity Hospital in New Orleans transverse Kirschner wires have been routinely used to stabilize the zygoma in these cases. Although this technic has been described previously, its indications, advantages, and limitations are not widely appreciated. Eight recent cases are presented to demonstrate the technic, and alternative methods of treatment are discussed.", "contents": "Use of transverse Kirschner wires in comminuted facial fractures. Although most facial fractures can be well managed with open reduction and direct wiring of the fracture sites, this treatment is inadequate for some comminuted fractures and further fixation is required. At Charity Hospital in New Orleans transverse Kirschner wires have been routinely used to stabilize the zygoma in these cases. Although this technic has been described previously, its indications, advantages, and limitations are not widely appreciated. Eight recent cases are presented to demonstrate the technic, and alternative methods of treatment are discussed."}
{"id": "PMID:482985", "title": "Fungus balls of the urinary tract.", "content": "Fungus balls of the urinary tract are rare and usually associated with infection by Candid albicans. Since 1968 five patients seen at the Medical College of Virginia Hospitals presented with this peculiar manifestation of candidiasis. Summaries of their epidemiologic clinical, pathologic, and mycologic data are presented. All Candida fungus balls involved the upper collecting system and were detected by radiography and confirmed by culture and/or pathologic section. Two of the five patients completely recovered. Three patients were treated with flucytosine and/or local irrigation with a polyene antifungal agent. Two recovered and the third died of probable bacterial sepsis. One patient was treated successfully with surgical removal of the fungus ball and a brief period of local irrigation with amphotericin B (AMB). The fifth patient recovered after 28 days of parenteral AMB. Predisposing factors and pathogenetic mechanisms are discussed, and a rational approach to therapy is outlined.", "contents": "Fungus balls of the urinary tract. Fungus balls of the urinary tract are rare and usually associated with infection by Candid albicans. Since 1968 five patients seen at the Medical College of Virginia Hospitals presented with this peculiar manifestation of candidiasis. Summaries of their epidemiologic clinical, pathologic, and mycologic data are presented. All Candida fungus balls involved the upper collecting system and were detected by radiography and confirmed by culture and/or pathologic section. Two of the five patients completely recovered. Three patients were treated with flucytosine and/or local irrigation with a polyene antifungal agent. Two recovered and the third died of probable bacterial sepsis. One patient was treated successfully with surgical removal of the fungus ball and a brief period of local irrigation with amphotericin B (AMB). The fifth patient recovered after 28 days of parenteral AMB. Predisposing factors and pathogenetic mechanisms are discussed, and a rational approach to therapy is outlined."}
{"id": "PMID:482986", "title": "Evaluation of the gallbladder by gray scale ultrasonography.", "content": "A review of the results of the evaluation of the gallbladder by gray scale ultrasonography in 52 patients is presented. When the gallbladder was successfully identified by ultrasound (46 patients) the detection of stones was 98% accurate. When the gallbladder was not identified (four patients), a contracted gallbladder containing numerous stones was found at operation. The left lateral decubitus position proved very helpful in displaying the gallbladder when it could not be identified in the standard supine position. Ultrasonic evaluation of the gallbladder is of most value in the patients who have had unsuccessful radiographic studies.", "contents": "Evaluation of the gallbladder by gray scale ultrasonography. A review of the results of the evaluation of the gallbladder by gray scale ultrasonography in 52 patients is presented. When the gallbladder was successfully identified by ultrasound (46 patients) the detection of stones was 98% accurate. When the gallbladder was not identified (four patients), a contracted gallbladder containing numerous stones was found at operation. The left lateral decubitus position proved very helpful in displaying the gallbladder when it could not be identified in the standard supine position. Ultrasonic evaluation of the gallbladder is of most value in the patients who have had unsuccessful radiographic studies."}
{"id": "PMID:482987", "title": "Treatment of Goodpasture's syndrome with immunosuppression and plasmapheresis.", "content": "A 14-year-old boy with Goodpasture's syndrome induced by anti-glomerular-basement-membrane (gmb) antibody exhibited declining renal function, in association with a progressive increase in the level of serum anti-GBM antibody. Treatment with prednisone, cyclophosphamide, and plasmapheresis was associated with rapid disappearance of the serum anti-GMB antibody and temporary stabilization of renal function.", "contents": "Treatment of Goodpasture's syndrome with immunosuppression and plasmapheresis. A 14-year-old boy with Goodpasture's syndrome induced by anti-glomerular-basement-membrane (gmb) antibody exhibited declining renal function, in association with a progressive increase in the level of serum anti-GBM antibody. Treatment with prednisone, cyclophosphamide, and plasmapheresis was associated with rapid disappearance of the serum anti-GMB antibody and temporary stabilization of renal function."}
{"id": "PMID:482988", "title": "Simultaneous open prostatectomy and inguinal herniorrhaphy.", "content": "We report our results in 18 patients who had simultaneous open prostatectomy and inguinal herniorrhaphy between 1969 and 1976, and present our criteria for patient selection, operative technic, and postoperative results. The incidence of postoperative wound infection and recurrent hernia was 5.6% and 0 respectively, which compares favorably to results of herniorrhaphy and prostatectomy performed separately. The historic development of this procedure and the results of previously reported series are reviewed.", "contents": "Simultaneous open prostatectomy and inguinal herniorrhaphy. We report our results in 18 patients who had simultaneous open prostatectomy and inguinal herniorrhaphy between 1969 and 1976, and present our criteria for patient selection, operative technic, and postoperative results. The incidence of postoperative wound infection and recurrent hernia was 5.6% and 0 respectively, which compares favorably to results of herniorrhaphy and prostatectomy performed separately. The historic development of this procedure and the results of previously reported series are reviewed."}
{"id": "PMID:482989", "title": "Chronic recurrent angina.", "content": "The natural history of variant angina is described in a patient with multiple exacerbations and remissions over a period of several years. Constant in-hospital electrocardiographic monitoring for 26 days documented 569 episodes of ST elevation, 89% of which were asymptomatic. Episodes ranged in duration from 15 seconds to 11 1/2 minutes, with 4% of episodes associated with premature ventricular contractions. Over the course of hospitalization, the episodes of ST elevation decreased in frequency and length, as did episodes of pain. During this period, a number of therapeutic agents, including propranolol, indomethacin, and chlorpheniramine were evaluated using double-blind crossover trials. No agent had a significant beneficial effect on the course of the disease, and there was an increase in the length of episodes with high doses of propranolol. Because of the great variability in the course of this disease, only double-blind crossover trials should be allowed to dictate the efficacy of therapeutic agents.", "contents": "Chronic recurrent angina. The natural history of variant angina is described in a patient with multiple exacerbations and remissions over a period of several years. Constant in-hospital electrocardiographic monitoring for 26 days documented 569 episodes of ST elevation, 89% of which were asymptomatic. Episodes ranged in duration from 15 seconds to 11 1/2 minutes, with 4% of episodes associated with premature ventricular contractions. Over the course of hospitalization, the episodes of ST elevation decreased in frequency and length, as did episodes of pain. During this period, a number of therapeutic agents, including propranolol, indomethacin, and chlorpheniramine were evaluated using double-blind crossover trials. No agent had a significant beneficial effect on the course of the disease, and there was an increase in the length of episodes with high doses of propranolol. Because of the great variability in the course of this disease, only double-blind crossover trials should be allowed to dictate the efficacy of therapeutic agents."}
{"id": "PMID:482990", "title": "Health and nutritional status of Vietnamese refugees.", "content": "There are presently more than 7,000 Vietnamese refugees living in the greater New Orleans area. This paper summarizes the health and information on nutrition now available regarding Vietnamese refugees living in the United States. It is hoped that this effort will add to the documentation of the health changes occurring in migrant populations, and also stimulate the development of programs which will improve the health of the Vietnamese refugees in the United States. Although information on both the national and local level is limited, the following problems are found frequently in the Vietnamese refugee population in this country: dental caries, skin infestations, lack of adequate immunizations, and tuberculosis. Very little information is available concerning dietary habits and their relation to growth and development. There is some evidence that American nutritional patterns are being superimposed on traditional Vietnamese patterns. Target areas for further research are suggested.", "contents": "Health and nutritional status of Vietnamese refugees. There are presently more than 7,000 Vietnamese refugees living in the greater New Orleans area. This paper summarizes the health and information on nutrition now available regarding Vietnamese refugees living in the United States. It is hoped that this effort will add to the documentation of the health changes occurring in migrant populations, and also stimulate the development of programs which will improve the health of the Vietnamese refugees in the United States. Although information on both the national and local level is limited, the following problems are found frequently in the Vietnamese refugee population in this country: dental caries, skin infestations, lack of adequate immunizations, and tuberculosis. Very little information is available concerning dietary habits and their relation to growth and development. There is some evidence that American nutritional patterns are being superimposed on traditional Vietnamese patterns. Target areas for further research are suggested."}
{"id": "PMID:482992", "title": "The importance of the doctor to the dying patient.", "content": "The physician plays a central role in the emotional as well as the physical care of the dying patient. The concerns of the terminally ill cancer patient include (1) emotional response to the physical limitations of illness, (2) communication with significant others, (3) self-concept, and (4) resolution regarding impending death. Although a busy physician would not be able to spend much time talking with the patient about these concerns, the physician could communicate to the patient in just a few minutes an empathic understanding of the emotional crises the patient faces. Sometimes only a word or touch of the hand can covey the physician's support and understanding which are so meaningful for a terminally ill patient.", "contents": "The importance of the doctor to the dying patient. The physician plays a central role in the emotional as well as the physical care of the dying patient. The concerns of the terminally ill cancer patient include (1) emotional response to the physical limitations of illness, (2) communication with significant others, (3) self-concept, and (4) resolution regarding impending death. Although a busy physician would not be able to spend much time talking with the patient about these concerns, the physician could communicate to the patient in just a few minutes an empathic understanding of the emotional crises the patient faces. Sometimes only a word or touch of the hand can covey the physician's support and understanding which are so meaningful for a terminally ill patient."}
{"id": "PMID:482993", "title": "Idiopathic arterial calcification of infancy.", "content": "Idiopathic arterial calcification of infancy is an uncommon disease of unknown etiology, characterized by calcification along the internal elastic membrane and intimal proliferation of almost all the arteries of the body. When the diagnosis is made premortem, it is usually done by radiographic demonstration of arterial calcification. Because of its edge enhancement effect, xeroradiography should be useful in detecting the soft tissue calcifications of this disease.", "contents": "Idiopathic arterial calcification of infancy. Idiopathic arterial calcification of infancy is an uncommon disease of unknown etiology, characterized by calcification along the internal elastic membrane and intimal proliferation of almost all the arteries of the body. When the diagnosis is made premortem, it is usually done by radiographic demonstration of arterial calcification. Because of its edge enhancement effect, xeroradiography should be useful in detecting the soft tissue calcifications of this disease."}
{"id": "PMID:482994", "title": "Familial gynecomastia without hypogonadism.", "content": "A family, six members of which had gynecomastia without hypogonadism, is presented. Endocrine studies failed to show the specific nature of breast enlargement in the patients. Chromosomal studies using peripheral leukocytes resulted in normal findings. In one of the patients, chromosomal studies were done using breast tissue and fascia, and again no abnormalities could be detected. The mode of inheritance may be autosoma dominant with sex limitations; however, X-linked inheritance cannot be ruled out.", "contents": "Familial gynecomastia without hypogonadism. A family, six members of which had gynecomastia without hypogonadism, is presented. Endocrine studies failed to show the specific nature of breast enlargement in the patients. Chromosomal studies using peripheral leukocytes resulted in normal findings. In one of the patients, chromosomal studies were done using breast tissue and fascia, and again no abnormalities could be detected. The mode of inheritance may be autosoma dominant with sex limitations; however, X-linked inheritance cannot be ruled out."}
{"id": "PMID:482995", "title": "Noncirrhotic portal hypertension after arsenic ingestion.", "content": "Five years after arsenic therapy, the patient described had noncirrhotic portal hypertension, for which he had splenectomy and anastomosis of the splenic vein to the left renal vein. During the 12-year postoperative period he had Bowen's disease (skin carcinoma), but has had normal liver function and no further gastrointestinal bleeding. Arsenic exposure in humans is common throughout the world and may lead to late complications such as noncirrhotic portal hypertension and skin carcinoma, as well as malignancies of the lungs, liver, and lymphatic systems. It may also lead to severe arteriosclerosis with involvement of the heart and extremities.", "contents": "Noncirrhotic portal hypertension after arsenic ingestion. Five years after arsenic therapy, the patient described had noncirrhotic portal hypertension, for which he had splenectomy and anastomosis of the splenic vein to the left renal vein. During the 12-year postoperative period he had Bowen's disease (skin carcinoma), but has had normal liver function and no further gastrointestinal bleeding. Arsenic exposure in humans is common throughout the world and may lead to late complications such as noncirrhotic portal hypertension and skin carcinoma, as well as malignancies of the lungs, liver, and lymphatic systems. It may also lead to severe arteriosclerosis with involvement of the heart and extremities."}
{"id": "PMID:482996", "title": "Urticaria subsequent to administration of influenza vaccine.", "content": "Described is an 11-year-old girl who had a reaction including urticaria and asthma to influenza immunization. The urticaria lasted for nine days. This patient had no clinical sensitivity to egg and no skin test reactivity to egg, but she did show positive skin test reactions to influenza B and bivalent flu vaccine. Since passive transfer did not produce positive skin tests, it would appear that the reactions were not IgE-mediated. The patient has not had subsequent urticaria. The possibility exists of reactions to influenza virus antigens or bacterial or other contaminants of the vaccine. More complete and detailed information about \"contaminants\" in influenza (and other egg embryo) vaccines should be made available so that these factors can be taken into consideration in seeking the cause of reactions to these vaccines and in possibly preventing reactions.", "contents": "Urticaria subsequent to administration of influenza vaccine. Described is an 11-year-old girl who had a reaction including urticaria and asthma to influenza immunization. The urticaria lasted for nine days. This patient had no clinical sensitivity to egg and no skin test reactivity to egg, but she did show positive skin test reactions to influenza B and bivalent flu vaccine. Since passive transfer did not produce positive skin tests, it would appear that the reactions were not IgE-mediated. The patient has not had subsequent urticaria. The possibility exists of reactions to influenza virus antigens or bacterial or other contaminants of the vaccine. More complete and detailed information about \"contaminants\" in influenza (and other egg embryo) vaccines should be made available so that these factors can be taken into consideration in seeking the cause of reactions to these vaccines and in possibly preventing reactions."}
{"id": "PMID:482998", "title": "Extravasation of contrast medium from traumatic laceration of the intracerebral internal carotid artery.", "content": "A single case of traumatic laceration of the intracerebral internal carotid artery diagnosed by extravasation of contrast medium is presented. The patient had associated cerebral circulatory arrest caused by increased intracranial pressure. A similar documentation has been made in only three previous cases.", "contents": "Extravasation of contrast medium from traumatic laceration of the intracerebral internal carotid artery. A single case of traumatic laceration of the intracerebral internal carotid artery diagnosed by extravasation of contrast medium is presented. The patient had associated cerebral circulatory arrest caused by increased intracranial pressure. A similar documentation has been made in only three previous cases."}
{"id": "PMID:482999", "title": "Isolated bronchial capillary hemangioma: a rare benign cause of hemoptysis.", "content": "A 74-year-old male cigarette smoker presented with hemoptysis and a right upper lobe infiltrate. Fiberoptic bronchoscopy revealed a small (1 x 2 mm) benign-appearing lesion in the right upper lobe bronchus which, on biopsy, was a submucosal capillary hemangioma. This is an exceedingly rare tumor and, to our knowledge, hemoptysis from these lesions in the proximal bronchi has not been previously reported.", "contents": "Isolated bronchial capillary hemangioma: a rare benign cause of hemoptysis. A 74-year-old male cigarette smoker presented with hemoptysis and a right upper lobe infiltrate. Fiberoptic bronchoscopy revealed a small (1 x 2 mm) benign-appearing lesion in the right upper lobe bronchus which, on biopsy, was a submucosal capillary hemangioma. This is an exceedingly rare tumor and, to our knowledge, hemoptysis from these lesions in the proximal bronchi has not been previously reported."}
{"id": "PMID:483000", "title": "Stercoraceous perforation of the colon.", "content": "Stercoraceous perforation of the colon is most often unsuspected and is usually diagnosed at operation or autopsy. Abdominal radiographs show large fecal masses in the rectum and sigmoid colon, and occasionally lying free in the peritoneal cavity. Free intraperitoneal air is present. We have reported a single case, with a discussion of the pathophysiology.", "contents": "Stercoraceous perforation of the colon. Stercoraceous perforation of the colon is most often unsuspected and is usually diagnosed at operation or autopsy. Abdominal radiographs show large fecal masses in the rectum and sigmoid colon, and occasionally lying free in the peritoneal cavity. Free intraperitoneal air is present. We have reported a single case, with a discussion of the pathophysiology."}
{"id": "PMID:483001", "title": "Alcohol-induced Cushingoid syndrome.", "content": "A 35-year-old woman with alcoholic liver disease presented with physical stigmas and laboratory confirmation of Cushing's syndrome. With discontinuance of alcohol, her signs of Cushing's syndrome resolved and the urinary free cortisol and the urinary 17-hydroxycorticosteroid response to dexamethasone returned to normal. These findings broaden the spectrum of deranged glucocorticoid chemistry that can occur in alcohol-induced cushingoid syndrome.", "contents": "Alcohol-induced Cushingoid syndrome. A 35-year-old woman with alcoholic liver disease presented with physical stigmas and laboratory confirmation of Cushing's syndrome. With discontinuance of alcohol, her signs of Cushing's syndrome resolved and the urinary free cortisol and the urinary 17-hydroxycorticosteroid response to dexamethasone returned to normal. These findings broaden the spectrum of deranged glucocorticoid chemistry that can occur in alcohol-induced cushingoid syndrome."}
{"id": "PMID:483002", "title": "Hernia of Morgagni's foramen presenting as asymptomatic pleural mass.", "content": "An asymptomatic 64-year-old black man presented with an enlarging left pleural mass. Preoperative evaluation failed to reveal an etiology. At thoractomy omental fat was found along the diaphragm, extending to the left costophrenic angle, and protruding through a hernia of Morgagni's foramen. The omentum was replaced into the abdomen and the defect was repaired.", "contents": "Hernia of Morgagni's foramen presenting as asymptomatic pleural mass. An asymptomatic 64-year-old black man presented with an enlarging left pleural mass. Preoperative evaluation failed to reveal an etiology. At thoractomy omental fat was found along the diaphragm, extending to the left costophrenic angle, and protruding through a hernia of Morgagni's foramen. The omentum was replaced into the abdomen and the defect was repaired."}
{"id": "PMID:483004", "title": "Prevalence of anti-HBc in Malaysian male blood donors and its correlation with DNA polymerase activity.", "content": "A study of Kuala Lumpur blood donors for HBsAG, anti-HBc and DNA polymeraes showed that 5.5% in the sample population was positive for HBsAG, 50.1% for anti-HBc and 10.1% for DNA polymerase activity. There was no significant difference of the HBsAG among the Malay, Chinese and Indian groups. However, a significant difference was observed for the anti-HBc and DNA polymerase activity between the Indian and the Malay/Chinese groups. Both analysis were significantly lower in the Indians but there was no significant difference between the Chinese and the Malays.", "contents": "Prevalence of anti-HBc in Malaysian male blood donors and its correlation with DNA polymerase activity. A study of Kuala Lumpur blood donors for HBsAG, anti-HBc and DNA polymeraes showed that 5.5% in the sample population was positive for HBsAG, 50.1% for anti-HBc and 10.1% for DNA polymerase activity. There was no significant difference of the HBsAG among the Malay, Chinese and Indian groups. However, a significant difference was observed for the anti-HBc and DNA polymerase activity between the Indian and the Malay/Chinese groups. Both analysis were significantly lower in the Indians but there was no significant difference between the Chinese and the Malays."}
{"id": "PMID:483005", "title": "Endemicity of cholera in Surabaya, Indonesia.", "content": "As the seventh pandemic of cholera is caused by V. cholerae biotype El Tor, the former criteria for endemicity of cholera need to be reconsidered as regards their applicability in areas that are infected with cholera. As the mortality rate of cholera nowadays can be reduced to a very low level due to modern methods of treatment, it is suggested that the infection rates of cholera should be taken into consideration as criteria of cholera endemicity, i.e. 1. Five years persistence of cholera cases in a given area. 2. Five percent infection rate among family contacts of cholera cases. 3. Minimum infection rate of 1% in a vicinity where cholera cases occur. It was also found that in such an endemic area it is very difficult to eliminate V. cholerae infection from a locality, even when all family contacts are treated with the full dose of tetracycline.", "contents": "Endemicity of cholera in Surabaya, Indonesia. As the seventh pandemic of cholera is caused by V. cholerae biotype El Tor, the former criteria for endemicity of cholera need to be reconsidered as regards their applicability in areas that are infected with cholera. As the mortality rate of cholera nowadays can be reduced to a very low level due to modern methods of treatment, it is suggested that the infection rates of cholera should be taken into consideration as criteria of cholera endemicity, i.e. 1. Five years persistence of cholera cases in a given area. 2. Five percent infection rate among family contacts of cholera cases. 3. Minimum infection rate of 1% in a vicinity where cholera cases occur. It was also found that in such an endemic area it is very difficult to eliminate V. cholerae infection from a locality, even when all family contacts are treated with the full dose of tetracycline."}
{"id": "PMID:483006", "title": "Life expectancy in an aboriginal Malaysian population.", "content": "A life table for an aboriginal Malaysian population, the Semelai, living in West Malaysia, was constructed using censuses from 1965, 1969, and 1974; and interview data from 1974. The life expectancy at birth for this population, 54.0 years, was compared to that of other Malaysian populations and selected Asian populations. This comparison indicated that the Semelai were at a disadvantage compared to the Malaysian populations, but in a favorable position when compared with the other Asian populations.", "contents": "Life expectancy in an aboriginal Malaysian population. A life table for an aboriginal Malaysian population, the Semelai, living in West Malaysia, was constructed using censuses from 1965, 1969, and 1974; and interview data from 1974. The life expectancy at birth for this population, 54.0 years, was compared to that of other Malaysian populations and selected Asian populations. This comparison indicated that the Semelai were at a disadvantage compared to the Malaysian populations, but in a favorable position when compared with the other Asian populations."}
{"id": "PMID:483007", "title": "Parasite fauna of the house rat Rattus rattus diardii in Kuala Lumpur and nearby villages.", "content": "One hundred and fifty one house rats, Rattus rattus diardii from five different localities, Jinjang, Dato Keramat, Kuala Lumpur, Sungai Besi and Selayang Baru, were examined for parasites. Nineteen species of parasites were recovered. Hymenolepis diminuta and Nippostrongylus brasiliensis are the predominant species. The dominancy of the parasite species in the rats differed in each locality: Hymenolepis diminuta in Dato Keramat and Kuala Lumpur; Nippostrongylus brasiliensis in Sungai Besi; Gongylomena neoplasticum in Jinjang and Selayang Baru. The influences of human habitats on the parasite fauna of house rats are discussed.", "contents": "Parasite fauna of the house rat Rattus rattus diardii in Kuala Lumpur and nearby villages. One hundred and fifty one house rats, Rattus rattus diardii from five different localities, Jinjang, Dato Keramat, Kuala Lumpur, Sungai Besi and Selayang Baru, were examined for parasites. Nineteen species of parasites were recovered. Hymenolepis diminuta and Nippostrongylus brasiliensis are the predominant species. The dominancy of the parasite species in the rats differed in each locality: Hymenolepis diminuta in Dato Keramat and Kuala Lumpur; Nippostrongylus brasiliensis in Sungai Besi; Gongylomena neoplasticum in Jinjang and Selayang Baru. The influences of human habitats on the parasite fauna of house rats are discussed."}
{"id": "PMID:483008", "title": "Tachypleus lysate test for endotoxin in patients with Gram negative bacterial infections.", "content": "Amoebocyte lysate from the horseshoe crabs (Tachypleus gigas) which abounds in the Gulf of Thailand was used to detect endotoxin in patients with Gram-negative bacteremia, in patients with Gram-positive bacterial infections as well as in the control. The Tachypleus lysate test (TLT) was positive in 94.4% of 36 patients with Gram-negative bacteremia before initiation of antibiotic therapy. Only 4% of 50 healthy individuals were positive and all 7 patients with Gram-positive bacterial infections were negative. The threshold sensitivity of TLT was 0.625 micrograms endotoxin per ml of the plasma. In comparison with the commercial Limulus lysate test (LLT), the TLT was slightly more sensitive in exhibiting higher grade of reaction, eventhough the threshold sensitivity was the same.", "contents": "Tachypleus lysate test for endotoxin in patients with Gram negative bacterial infections. Amoebocyte lysate from the horseshoe crabs (Tachypleus gigas) which abounds in the Gulf of Thailand was used to detect endotoxin in patients with Gram-negative bacteremia, in patients with Gram-positive bacterial infections as well as in the control. The Tachypleus lysate test (TLT) was positive in 94.4% of 36 patients with Gram-negative bacteremia before initiation of antibiotic therapy. Only 4% of 50 healthy individuals were positive and all 7 patients with Gram-positive bacterial infections were negative. The threshold sensitivity of TLT was 0.625 micrograms endotoxin per ml of the plasma. In comparison with the commercial Limulus lysate test (LLT), the TLT was slightly more sensitive in exhibiting higher grade of reaction, eventhough the threshold sensitivity was the same."}
{"id": "PMID:483016", "title": "Microfilarial periodicity analysis of the survey data from six localities in Indonesia.", "content": "The survey data of microfilarial periodicity of 2 species of filariae Brugia malayi at 4 localities, Mahang and Lampihong in South Kalimantan, Bengkulu in South Sumatra, Palolo in Central Sulwesi, and the species of Brugia timori at 2 localities, Flores and Alor Islands in Nusa Tenggara were analysed by using Aikat and Das's modified statistical method. Nine cases at Mahang and five cases at Lampihong were recognized as same group of non-periodic B. malayi and 1 case at Mahang was considered to be nocturnal subperiodic B. malayi by their parameters estimated by the wave equation, the amplitude (a), the peak hour of microfilaria density (k) the periodicity index (D) and the test of equality for comparison of two series of data. The data of 9 cases from Bengkulu and 11 cases from Palolo were similarly analysed and recognized as the nocturnal periodic type of B. malayi. The data of 5 cases from Flores Island and 5 cases from Alor Island were also recognized as the nocturanal periodic type of B. timori. Thus, in these observations the non periodic strain of Brugia malayi was detected in South Kalimantan. Further studies on the principal vectors and their bionomics should be carried out.", "contents": "Microfilarial periodicity analysis of the survey data from six localities in Indonesia. The survey data of microfilarial periodicity of 2 species of filariae Brugia malayi at 4 localities, Mahang and Lampihong in South Kalimantan, Bengkulu in South Sumatra, Palolo in Central Sulwesi, and the species of Brugia timori at 2 localities, Flores and Alor Islands in Nusa Tenggara were analysed by using Aikat and Das's modified statistical method. Nine cases at Mahang and five cases at Lampihong were recognized as same group of non-periodic B. malayi and 1 case at Mahang was considered to be nocturnal subperiodic B. malayi by their parameters estimated by the wave equation, the amplitude (a), the peak hour of microfilaria density (k) the periodicity index (D) and the test of equality for comparison of two series of data. The data of 9 cases from Bengkulu and 11 cases from Palolo were similarly analysed and recognized as the nocturnal periodic type of B. malayi. The data of 5 cases from Flores Island and 5 cases from Alor Island were also recognized as the nocturanal periodic type of B. timori. Thus, in these observations the non periodic strain of Brugia malayi was detected in South Kalimantan. Further studies on the principal vectors and their bionomics should be carried out."}
{"id": "PMID:483017", "title": "Field evaluation of dimethoate as a systemic for the control of chigger mites in the Pescadores Island of Taiwan.", "content": "A 0.25% concentration of dimethoate applied as a surface coat on bait grain was evaluated in an open field trial in the Pescadores Islands of Taiwan for the systemic control of chigger mites. Unsatisfactory levels of control were observed on all 4 species of mammals studied. Chigger control with systemics does not appear practical at this time.", "contents": "Field evaluation of dimethoate as a systemic for the control of chigger mites in the Pescadores Island of Taiwan. A 0.25% concentration of dimethoate applied as a surface coat on bait grain was evaluated in an open field trial in the Pescadores Islands of Taiwan for the systemic control of chigger mites. Unsatisfactory levels of control were observed on all 4 species of mammals studied. Chigger control with systemics does not appear practical at this time."}
{"id": "PMID:483018", "title": "Serum vitamin B 12 and folic acid levels in patients with fasciolopsiasis.", "content": "Serum vitamin B12, serum and red cell folate concentrations and vitamin B12 absorption were studied in 100 patients with fasciolopsiasis. A mean value of serum vitamin B12 level in the patient group was found to be significantly lower than that of normal subjects and 14% of these patients had serum vitamin B12 level less than 100 pg/ml. Serum UBBC and TBBC levels in the patients were significantly higher than those of the normal subjects. Serum TCI and TCIII increased significantly while TCII decreased. Vitamin B12 absorption was found to be impaired in 3 of 9 patients studied. There was no relationship seen between serum vitamin B12 level and vitamin B12 absorption. The mean values of serum folate and red cell folate levels in the patient group were significantly lower than those of normal subjects. Fifteen of 100 patients (15%) had serum folate level less than 3 ng/ml, while all of them had red cell folate higher than 100 ng/ml. Serum folic acid binding protein levels (FABP) in these patients, were not significantly different from those of normal subjects.", "contents": "Serum vitamin B 12 and folic acid levels in patients with fasciolopsiasis. Serum vitamin B12, serum and red cell folate concentrations and vitamin B12 absorption were studied in 100 patients with fasciolopsiasis. A mean value of serum vitamin B12 level in the patient group was found to be significantly lower than that of normal subjects and 14% of these patients had serum vitamin B12 level less than 100 pg/ml. Serum UBBC and TBBC levels in the patients were significantly higher than those of the normal subjects. Serum TCI and TCIII increased significantly while TCII decreased. Vitamin B12 absorption was found to be impaired in 3 of 9 patients studied. There was no relationship seen between serum vitamin B12 level and vitamin B12 absorption. The mean values of serum folate and red cell folate levels in the patient group were significantly lower than those of normal subjects. Fifteen of 100 patients (15%) had serum folate level less than 3 ng/ml, while all of them had red cell folate higher than 100 ng/ml. Serum folic acid binding protein levels (FABP) in these patients, were not significantly different from those of normal subjects."}
{"id": "PMID:483019", "title": "Diagnostic value of buffy coat preparation in dengue hemorrhagic fever.", "content": "Examination of the buffy coat preparation is a simple, rapid diagnostic aid for the early diagnosis of dengue hemorrhagic fever (DHF). The presence of many transformed lymphocytes, usually 20-50 per cent during the first few days of fever is a unique finding especially during a secondary infection. Similar findings are absent during the early phase of illness in other viral infections. The buffy coat appearance in DHF is also clearly different from those in patients with bacterial infections, which show many polymorphonuclear leukocytes containing toxic granules and vacuoles. The early buffy coat diagnosis in 320 cases of DHF correlated well with the later serologic diagnosis. The buffy coat diagnosis of secondary DHF was correct in 94.2% of cases as early as the third day of fever. This technique has proven to be clinically useful for early screening test of secondary DHF in Southeast Asian countries. The observation of many transformed  lymphocytes in the peripheral blood of DHF patients also offer some insight into the pathogenesis of this condition.", "contents": "Diagnostic value of buffy coat preparation in dengue hemorrhagic fever. Examination of the buffy coat preparation is a simple, rapid diagnostic aid for the early diagnosis of dengue hemorrhagic fever (DHF). The presence of many transformed lymphocytes, usually 20-50 per cent during the first few days of fever is a unique finding especially during a secondary infection. Similar findings are absent during the early phase of illness in other viral infections. The buffy coat appearance in DHF is also clearly different from those in patients with bacterial infections, which show many polymorphonuclear leukocytes containing toxic granules and vacuoles. The early buffy coat diagnosis in 320 cases of DHF correlated well with the later serologic diagnosis. The buffy coat diagnosis of secondary DHF was correct in 94.2% of cases as early as the third day of fever. This technique has proven to be clinically useful for early screening test of secondary DHF in Southeast Asian countries. The observation of many transformed  lymphocytes in the peripheral blood of DHF patients also offer some insight into the pathogenesis of this condition."}
{"id": "PMID:483020", "title": "The study of intestinal immunity against V. cholerae: purification of V. cholerae El Tor haemagglutinin and the protective role of its antibody in experimental cholera.", "content": "V. cholerae El Tor Ogawa strain O17SR grown on trypticase soy agar were extracted with 0.05 M cyclohexylamino propane sulfonic acid (CAPS) pH 9.5 at 37 degrees C for 1 hour. The bacteria were then removed by centrifugation and millipore filtration. The filtered fluid, after being dialysed against many changes of cold distilled water, was concentrated and passed through Sephadex G200 column. Three protein profiles were eluted out with 0.05 M Tris buffer pH 8.6. The haemagglutinin and the bacterial lipopolysaccharide (LPS) were confined to the first profile. They were subsequently separated by agarose gel electrophoresis. The haemagglutinin was found to be more anodic than the LPS. After homogenization of the gel strips containing the haemagglutinin followed by centrifugation at 9,000 g pure haemagglutinin was obtained in the supernatant. Rabbit aniserum against pure haemagglutinin contained protective antibodies against V. cholerae infection in the baby mouse model. Specific antibodies prepared from this antiserum was as protective as the antibodies directed against whole V. cholerae and heat stable somatic antigens of V. cholerae upon the same weight unit.", "contents": "The study of intestinal immunity against V. cholerae: purification of V. cholerae El Tor haemagglutinin and the protective role of its antibody in experimental cholera. V. cholerae El Tor Ogawa strain O17SR grown on trypticase soy agar were extracted with 0.05 M cyclohexylamino propane sulfonic acid (CAPS) pH 9.5 at 37 degrees C for 1 hour. The bacteria were then removed by centrifugation and millipore filtration. The filtered fluid, after being dialysed against many changes of cold distilled water, was concentrated and passed through Sephadex G200 column. Three protein profiles were eluted out with 0.05 M Tris buffer pH 8.6. The haemagglutinin and the bacterial lipopolysaccharide (LPS) were confined to the first profile. They were subsequently separated by agarose gel electrophoresis. The haemagglutinin was found to be more anodic than the LPS. After homogenization of the gel strips containing the haemagglutinin followed by centrifugation at 9,000 g pure haemagglutinin was obtained in the supernatant. Rabbit aniserum against pure haemagglutinin contained protective antibodies against V. cholerae infection in the baby mouse model. Specific antibodies prepared from this antiserum was as protective as the antibodies directed against whole V. cholerae and heat stable somatic antigens of V. cholerae upon the same weight unit."}
{"id": "PMID:483021", "title": "Scanning and transmission electronmicroscopy of Mekong Schistosoma eggs and adults.", "content": "Eggs and adult males and females of Mekong Schistosoma were studied by scanning and transmission electronmicroscopy. The observation of the eggs by the scanning and light microscopy revealed fine shell fenestration and a prominent knoblike spine. There are marked differences between the surface structures of male and female as studied by scanning and transmission electronmicroscopy. The surface of the male schistosome is moderately rough while that of the female is relatively smooth. SEM reveals certain basic features such as spines in the oral sucker, minute spines and folds in the gynecophoral canal of the male, and general features of male and female tegumental surfaces. The observations of the cross sections of adult schistosomes by transmission technique revealed certain features such as spines or ridges, and mucin droplets on the surface, the smooth muscles lining the integument, the mucin-producing cells and numerous lipid droplets in the body of the Schistosoma.", "contents": "Scanning and transmission electronmicroscopy of Mekong Schistosoma eggs and adults. Eggs and adult males and females of Mekong Schistosoma were studied by scanning and transmission electronmicroscopy. The observation of the eggs by the scanning and light microscopy revealed fine shell fenestration and a prominent knoblike spine. There are marked differences between the surface structures of male and female as studied by scanning and transmission electronmicroscopy. The surface of the male schistosome is moderately rough while that of the female is relatively smooth. SEM reveals certain basic features such as spines in the oral sucker, minute spines and folds in the gynecophoral canal of the male, and general features of male and female tegumental surfaces. The observations of the cross sections of adult schistosomes by transmission technique revealed certain features such as spines or ridges, and mucin droplets on the surface, the smooth muscles lining the integument, the mucin-producing cells and numerous lipid droplets in the body of the Schistosoma."}
{"id": "PMID:483043", "title": "Computed tomographic scanning and the lumbar spine. Part II: Clinical considerations.", "content": "With the advent of computed tomography of the lumbar spine, the theories of a small number of pioneering physicians regarding the pathologic sequela of degenerative disc disease and the entities of central and lateral spinal stenosis have been translated into objective determinations which can now be observed by all. CT scanning has provided clinicians with a better appreciation of the diagnostic limitations of myelography and of the pathologic sequela of hemilaminectomy, dorsal-lateral fusion, and discectomy itself. Since the identification by CT scanning of the loss of disc volume, leading to lateral nerve entrapment and nerve compression resulting from fusion overgrowth, it has become clear that our present modes of therapy require comprehensive reevaluation. Because of the findings of CT scanning, dorsal-lateral fusion now appears to have a much reduced role in the treatment of degenerative disc disease and spinal stenosis. In an era in which unnecessary surgery is a matter of concern, CT scanning is an important means of ensuring that surgery will be undertaken only after thorough pathologic conditions.", "contents": "Computed tomographic scanning and the lumbar spine. Part II: Clinical considerations. With the advent of computed tomography of the lumbar spine, the theories of a small number of pioneering physicians regarding the pathologic sequela of degenerative disc disease and the entities of central and lateral spinal stenosis have been translated into objective determinations which can now be observed by all. CT scanning has provided clinicians with a better appreciation of the diagnostic limitations of myelography and of the pathologic sequela of hemilaminectomy, dorsal-lateral fusion, and discectomy itself. Since the identification by CT scanning of the loss of disc volume, leading to lateral nerve entrapment and nerve compression resulting from fusion overgrowth, it has become clear that our present modes of therapy require comprehensive reevaluation. Because of the findings of CT scanning, dorsal-lateral fusion now appears to have a much reduced role in the treatment of degenerative disc disease and spinal stenosis. In an era in which unnecessary surgery is a matter of concern, CT scanning is an important means of ensuring that surgery will be undertaken only after thorough pathologic conditions."}
{"id": "PMID:483044", "title": "The significance and principles of computerized axial tomography in idiopathic developmental stenosis of the bony lumbar vertebral canal.", "content": "Computerized axial tomography (CAT) in cases of stenosis of the lumbar bony vertebral canal may demonstrate features of diagnostic significance in the shape of the canal and the bony structures surrounding it. The distinguishing features are the measurements. Standardized measurements of the midsagittal diameters at the cephalad and caudad borders of the laminas of the vertebral canal are proposed as a means of furthering both surgical and comparative studies. Identification of these borders in CAT scans is described, as is the procedure for taking measurements. One or two interlaminar, transarticular CAT scans are also made. In these scans, the midsagittal diameters cannot be measured because the ligamentum flavum forms the posterior wall in the midline. The scans may, however, demonstrate narrow lateral recesses and deformities of the articular processes and joint spaces. Errors in measurement of midsagittal diameters result from the inability of CT body scanners to show the angle between axial sections and the anterior vertebral canal wall, and from the partial volume phenomenon. The diameters are then too large. Errors in the angle of axial sections also lead to distortion in reconstructions of sagittal sections of the vertebral canal from the soft material obtained from axial sections. New precision designs are described. The principal indication for the intrathecal injection of water-soluble contrast media in CAT scanning is the determination of the thickness of the ligamentum flavum. Apart from its value in distinguishing various forms of stenosis, CAT scanning may also reveal inadequacies in surgical decompression.", "contents": "The significance and principles of computerized axial tomography in idiopathic developmental stenosis of the bony lumbar vertebral canal. Computerized axial tomography (CAT) in cases of stenosis of the lumbar bony vertebral canal may demonstrate features of diagnostic significance in the shape of the canal and the bony structures surrounding it. The distinguishing features are the measurements. Standardized measurements of the midsagittal diameters at the cephalad and caudad borders of the laminas of the vertebral canal are proposed as a means of furthering both surgical and comparative studies. Identification of these borders in CAT scans is described, as is the procedure for taking measurements. One or two interlaminar, transarticular CAT scans are also made. In these scans, the midsagittal diameters cannot be measured because the ligamentum flavum forms the posterior wall in the midline. The scans may, however, demonstrate narrow lateral recesses and deformities of the articular processes and joint spaces. Errors in measurement of midsagittal diameters result from the inability of CT body scanners to show the angle between axial sections and the anterior vertebral canal wall, and from the partial volume phenomenon. The diameters are then too large. Errors in the angle of axial sections also lead to distortion in reconstructions of sagittal sections of the vertebral canal from the soft material obtained from axial sections. New precision designs are described. The principal indication for the intrathecal injection of water-soluble contrast media in CAT scanning is the determination of the thickness of the ligamentum flavum. Apart from its value in distinguishing various forms of stenosis, CAT scanning may also reveal inadequacies in surgical decompression."}
{"id": "PMID:483045", "title": "Multiplanar computerized tomography in the normal spine and in the diagnosis of spinal stenosis. A gross anatomic-computerized tomographic correlation.", "content": "The limitations of current diagnostic tools, including myelography, in localizing the anatomic lesion in spinal stenosis are well recognized. The purpose of this study is to investigate whether computerized tomography can more effectively define the normal and abnormal osseous structures compromising the boundaries of the spinal canal, nerve root canal, and intervertebral foramen. This study is a pathologic-radiographic correlation between CT scans and sections of spine specimens that were so scanned. The tomographic images investigated were multiplanar, that is, simultaneously presenting transverse, coronal, and sagittal images. Spine specimens were chosen to show how the technique applies to the normal spine, as wel as to selected specimens with spinal stenosis. The role of the discs and apophyseal joints in shaping the intervertebral foramina and lateral recesses is emphasized in both normal and abnormal specimens. This study shows that computerized tomography can precisely localize anatomic lesions and, by comparison of the image to the gross anatomic specimen, that it is a reliable portrayal of the anatomic fact.", "contents": "Multiplanar computerized tomography in the normal spine and in the diagnosis of spinal stenosis. A gross anatomic-computerized tomographic correlation. The limitations of current diagnostic tools, including myelography, in localizing the anatomic lesion in spinal stenosis are well recognized. The purpose of this study is to investigate whether computerized tomography can more effectively define the normal and abnormal osseous structures compromising the boundaries of the spinal canal, nerve root canal, and intervertebral foramen. This study is a pathologic-radiographic correlation between CT scans and sections of spine specimens that were so scanned. The tomographic images investigated were multiplanar, that is, simultaneously presenting transverse, coronal, and sagittal images. Spine specimens were chosen to show how the technique applies to the normal spine, as wel as to selected specimens with spinal stenosis. The role of the discs and apophyseal joints in shaping the intervertebral foramina and lateral recesses is emphasized in both normal and abnormal specimens. This study shows that computerized tomography can precisely localize anatomic lesions and, by comparison of the image to the gross anatomic specimen, that it is a reliable portrayal of the anatomic fact."}
{"id": "PMID:483080", "title": "The respiratory depressive effects of intravenous buprenorphine in patients in an intensive care unit.", "content": "Buprenorphine hydrochloride, a new, potent, long-acting synthetic opiate analgesic, with partial agonist-antagonist activity, was administered intravenously to two groups of patients in an intensive care unit. Arterial blood was drawn for blood gas analysis before (control) and at regular intervals after drug administration, to determine the effects of intravenous buprenorphine on respiration in critically ill patients, each acting as his or her own control. Intravenous buprenorphine 0,4 mg (group I -- 10 patients) caused a significant reduction in mean respiration rate and an increase in mean PaCO2, but did not alter heart rate, PaO2 or base excess values. Intravenous buprenorphine 0,2 mg (group II -- 10 patients) was associated with a less significant reduction in the rate of breathing and elevation of PaCO2. Both 0,4 mg and 0,2 mg buprenorphine produced effective relief of pain, sedation, and reduction in restlessness, and allayed anxiety. Our results suggest that intravenous buprenorphine 0,2 mg can be safely recommended for the prolonged relief of postoperative pain in adults.", "contents": "The respiratory depressive effects of intravenous buprenorphine in patients in an intensive care unit. Buprenorphine hydrochloride, a new, potent, long-acting synthetic opiate analgesic, with partial agonist-antagonist activity, was administered intravenously to two groups of patients in an intensive care unit. Arterial blood was drawn for blood gas analysis before (control) and at regular intervals after drug administration, to determine the effects of intravenous buprenorphine on respiration in critically ill patients, each acting as his or her own control. Intravenous buprenorphine 0,4 mg (group I -- 10 patients) caused a significant reduction in mean respiration rate and an increase in mean PaCO2, but did not alter heart rate, PaO2 or base excess values. Intravenous buprenorphine 0,2 mg (group II -- 10 patients) was associated with a less significant reduction in the rate of breathing and elevation of PaCO2. Both 0,4 mg and 0,2 mg buprenorphine produced effective relief of pain, sedation, and reduction in restlessness, and allayed anxiety. Our results suggest that intravenous buprenorphine 0,2 mg can be safely recommended for the prolonged relief of postoperative pain in adults."}
{"id": "PMID:483081", "title": "Multiple squamous carcinomas and the oesophagus.", "content": "Attention is directed to the synchronous and sometimes delayed appearance of squamous carcinoma of the oesophagus with other head and neck squamous carcinomas. The rare occurrence of double carcinoma of the oesophagus is emphasized. Two cases of double carcinoma of the oesophagus occurred in a group of 383 patients who were investigated as part of an epidemiological survey (an incidence of 0,5%). A third case of double carcinoma of the oesophagus is additionally reported.", "contents": "Multiple squamous carcinomas and the oesophagus. Attention is directed to the synchronous and sometimes delayed appearance of squamous carcinoma of the oesophagus with other head and neck squamous carcinomas. The rare occurrence of double carcinoma of the oesophagus is emphasized. Two cases of double carcinoma of the oesophagus occurred in a group of 383 patients who were investigated as part of an epidemiological survey (an incidence of 0,5%). A third case of double carcinoma of the oesophagus is additionally reported."}
{"id": "PMID:483082", "title": "Observer bias and error in the integumentary clinical diagnosis of chronic anaemia.", "content": "In 74 patients believed to have chronic anaemia, 1 541 individual clinical assessments of haemoglobin levels were made from various sites. By comparison with laboratory values, there were statistically significant differences in the majority. In most instances these were not clinically important. The conjunctivae, the oral mucosa and the tongue were the integumentary sites that seemed to give the most accurate clinical assessment, while the appearance of nail-bed, palmar creases and facies did not correlate with laboratory findings. Bias appeared to be a factor in clinical estimates. There was no evidence that experience made clinicians more accurate.", "contents": "Observer bias and error in the integumentary clinical diagnosis of chronic anaemia. In 74 patients believed to have chronic anaemia, 1 541 individual clinical assessments of haemoglobin levels were made from various sites. By comparison with laboratory values, there were statistically significant differences in the majority. In most instances these were not clinically important. The conjunctivae, the oral mucosa and the tongue were the integumentary sites that seemed to give the most accurate clinical assessment, while the appearance of nail-bed, palmar creases and facies did not correlate with laboratory findings. Bias appeared to be a factor in clinical estimates. There was no evidence that experience made clinicians more accurate."}
{"id": "PMID:483083", "title": "Some aspects of middle ear surgery.", "content": "An eclectic, modified radical technique for the treatment of advanced middle ear and mastoid disease is described. This technique is safe and makes the ear easy to inspect. The reconstruction technique, using heterografts, homografts or alloplastic ossicular prostheses, minimizes lateralization or the tympanic membrane and provides satisfactory hearing results. From my experience the concept of Mr Bondy of a basic, modified radical technique for advanced ear lesions has withstood the test of time, and all credit is due to him.", "contents": "Some aspects of middle ear surgery. An eclectic, modified radical technique for the treatment of advanced middle ear and mastoid disease is described. This technique is safe and makes the ear easy to inspect. The reconstruction technique, using heterografts, homografts or alloplastic ossicular prostheses, minimizes lateralization or the tympanic membrane and provides satisfactory hearing results. From my experience the concept of Mr Bondy of a basic, modified radical technique for advanced ear lesions has withstood the test of time, and all credit is due to him."}
{"id": "PMID:483084", "title": "A follow-up study of cardiac patients. A preliminary report.", "content": "Five hundred consecutive admissions to a coronary care unit were studied, and the information was used to gain insight into the patients' background. It was found that the patients did not return for follow-up investigation. Contributory factors could have been that the patients did not understand their disease, and/or that some medical or paramedical services need to be reorganized. Diagnoses and other associated factors were also studied and related to one another statistically, in order to obtain a clearer understanding of the disease process as it affects individuals admitted to a coronary care unit.", "contents": "A follow-up study of cardiac patients. A preliminary report. Five hundred consecutive admissions to a coronary care unit were studied, and the information was used to gain insight into the patients' background. It was found that the patients did not return for follow-up investigation. Contributory factors could have been that the patients did not understand their disease, and/or that some medical or paramedical services need to be reorganized. Diagnoses and other associated factors were also studied and related to one another statistically, in order to obtain a clearer understanding of the disease process as it affects individuals admitted to a coronary care unit."}
{"id": "PMID:483085", "title": "Permanent pacemaker implantation in the absence of a right superior vena cava. A case report.", "content": "In a patient with syncope and sinus bradycardia the left superior vena cava drained into the right atrium via the coronary sinus. He underwent permanent transvenous pacemaker implantation via the tortuous left superior vena-caval route, and has continued to show normal pacing over an 18-month follow-up period. The problem of pre-operative recognition and the optimum means of permanent and temporary pacing in this condition are discussed.", "contents": "Permanent pacemaker implantation in the absence of a right superior vena cava. A case report. In a patient with syncope and sinus bradycardia the left superior vena cava drained into the right atrium via the coronary sinus. He underwent permanent transvenous pacemaker implantation via the tortuous left superior vena-caval route, and has continued to show normal pacing over an 18-month follow-up period. The problem of pre-operative recognition and the optimum means of permanent and temporary pacing in this condition are discussed."}
{"id": "PMID:483094", "title": "Which benzodiazepine, why and how?", "content": "While no major differences with regard to psychopharmacological actions are to be found among the benzodiazepines, certain pharmacokinetic differences are known. These differences allow the benzodiazepines to be classified as cumulative or non-cumulative; the differences between these two groups are further dissected and evaluated, in an attempt to rationalize therapy with these agents.", "contents": "Which benzodiazepine, why and how? While no major differences with regard to psychopharmacological actions are to be found among the benzodiazepines, certain pharmacokinetic differences are known. These differences allow the benzodiazepines to be classified as cumulative or non-cumulative; the differences between these two groups are further dissected and evaluated, in an attempt to rationalize therapy with these agents."}
{"id": "PMID:483095", "title": "The pathology of trachoma in a black South African population. Light microscopical, histochemical and electron microscopical findings.", "content": "Conjunctival biopsy specimens from a Black South African population suffering from trachoma have been studied by light microscopy, histochemistry and electron microscopy. A wide spectrum of histopathological changes was noted at different ages. Large cystically dilated glands containing concretions were seen and histochemical and electron microscopical examination revealed that the concretion material consisted of the inspissated secretion of goblet cells and cell debris. The cell damage seen in trachoma may be caused by the release of lysosomal enzymes from inflammatory cells.", "contents": "The pathology of trachoma in a black South African population. Light microscopical, histochemical and electron microscopical findings. Conjunctival biopsy specimens from a Black South African population suffering from trachoma have been studied by light microscopy, histochemistry and electron microscopy. A wide spectrum of histopathological changes was noted at different ages. Large cystically dilated glands containing concretions were seen and histochemical and electron microscopical examination revealed that the concretion material consisted of the inspissated secretion of goblet cells and cell debris. The cell damage seen in trachoma may be caused by the release of lysosomal enzymes from inflammatory cells."}
{"id": "PMID:483096", "title": "Does cimetidine alter the cardiac response to exercise and propranolol?", "content": "The recent discovery of histamine (H) receptors in the heart raises the possibility that the H2-antagonist drug, cimetidine, used in the therapy of peptic ulcer, might have cardiac side-effects and might impair the cardiac response to exercise. In 10 normal subjects, cimetidine did not alter the normal heart rate and blood pressure response to treadmill exercise, nor was the effect of beta-blockade by propranolol exaggerated. Thus it appears that the use of propranolol is not necessarily a contraindication to cimetidine therapy, or vice versa. However, further trials on patients with ischaemic heart disease are required to exclude any additive effects of cimetidine and propranolol on the diseased heart.", "contents": "Does cimetidine alter the cardiac response to exercise and propranolol? The recent discovery of histamine (H) receptors in the heart raises the possibility that the H2-antagonist drug, cimetidine, used in the therapy of peptic ulcer, might have cardiac side-effects and might impair the cardiac response to exercise. In 10 normal subjects, cimetidine did not alter the normal heart rate and blood pressure response to treadmill exercise, nor was the effect of beta-blockade by propranolol exaggerated. Thus it appears that the use of propranolol is not necessarily a contraindication to cimetidine therapy, or vice versa. However, further trials on patients with ischaemic heart disease are required to exclude any additive effects of cimetidine and propranolol on the diseased heart."}
{"id": "PMID:483097", "title": "Paroxysmal neurogenic hypertension and its prevention in patients with cervical spinal cord lesions.", "content": "Ten patients with clinically complete cervical spinal cord transection of traumatic origin were studied. These subjects do not have supraspinal control of their sympathetic outflow and are prone to marked elevations of blood pressure during visceral and somatic stimulation. This is a result of reflex sympathetic activity via the isolated spinal cord. The arterial blood pressures and heart rates of these patients were recorded on separate occasions during elevation of the urinary bladder pressure and before and after treatment with propranolol, labetalol, phenoxybenzamine and guanethidine. Guanethidine seems to afford the best protection against the marked hypertension occurring during autonomic hyperreflexia. In contrast to the ganglionic blocking agents, it has no anticholinergic or CNS side-effects, and reflex sweating, a valuable indicator of an impending abdominal catastrophe, still occurs. The findings that negative inotropic drugs, i.e. propranolol, guanethidine and labetalol, reduce the marked elevations in pulse pressure which occur during acute bladder distension in quadriplegic patients, suggest that inotropic cardiac responses are mediated by cardiac sympathetic nerves which leave the spinal cord above the T5 level.", "contents": "Paroxysmal neurogenic hypertension and its prevention in patients with cervical spinal cord lesions. Ten patients with clinically complete cervical spinal cord transection of traumatic origin were studied. These subjects do not have supraspinal control of their sympathetic outflow and are prone to marked elevations of blood pressure during visceral and somatic stimulation. This is a result of reflex sympathetic activity via the isolated spinal cord. The arterial blood pressures and heart rates of these patients were recorded on separate occasions during elevation of the urinary bladder pressure and before and after treatment with propranolol, labetalol, phenoxybenzamine and guanethidine. Guanethidine seems to afford the best protection against the marked hypertension occurring during autonomic hyperreflexia. In contrast to the ganglionic blocking agents, it has no anticholinergic or CNS side-effects, and reflex sweating, a valuable indicator of an impending abdominal catastrophe, still occurs. The findings that negative inotropic drugs, i.e. propranolol, guanethidine and labetalol, reduce the marked elevations in pulse pressure which occur during acute bladder distension in quadriplegic patients, suggest that inotropic cardiac responses are mediated by cardiac sympathetic nerves which leave the spinal cord above the T5 level."}
{"id": "PMID:483098", "title": "Mumps virus and ovarian cancer.", "content": "Thirty-four patients with carcinoma of the ovary were compared with controls matched for age, sex and racial origin. Previous mumps infection was determined by taking a history from the patient, by complement fixation test and by estimating neutralizing antibody titre. No significant differences between the two groups were found for any of the three methods used to estimate previous exposure to mumps virus. Therefore this study did not confirm previous hypotheses that mumps infection confers a significant degree of protection against the development of ovarian cancer. A relatively small proportion of cases could possibly be due to lack of such protection, but in this study not more than 30% at the 5% fiducial limit.", "contents": "Mumps virus and ovarian cancer. Thirty-four patients with carcinoma of the ovary were compared with controls matched for age, sex and racial origin. Previous mumps infection was determined by taking a history from the patient, by complement fixation test and by estimating neutralizing antibody titre. No significant differences between the two groups were found for any of the three methods used to estimate previous exposure to mumps virus. Therefore this study did not confirm previous hypotheses that mumps infection confers a significant degree of protection against the development of ovarian cancer. A relatively small proportion of cases could possibly be due to lack of such protection, but in this study not more than 30% at the 5% fiducial limit."}
{"id": "PMID:483099", "title": "Isolated fractures of the shaft of the ulna.", "content": "Fractures of the shaft of the ulna are described and a simple classification is proposed. Statistics are analysed in respect of mode of treatment and time of healing, and the unique mechanism of direct trauma is discussed. This survey showed that operative treatment has no place whatsoever in the treatment of these fractures.", "contents": "Isolated fractures of the shaft of the ulna. Fractures of the shaft of the ulna are described and a simple classification is proposed. Statistics are analysed in respect of mode of treatment and time of healing, and the unique mechanism of direct trauma is discussed. This survey showed that operative treatment has no place whatsoever in the treatment of these fractures."}
{"id": "PMID:483100", "title": "Cryosurgery for basal cell carcinoma of the head and neck.", "content": "Thirty patients with 57 basal cell carcinomas of the head and neck underwent cryosurgery during the years 1971--1973. Treatment was performed in the outpatient clinic, and no premedication or local anaesthesia was required. Residual disease necessitating local excision was found in 4 patients at follow-up which extended over a period of 2 years. The technique proved to be simple and safe. However, since tissue was not available for histological control, rigid criteria for patient selection and a prolonged follow-up are required.", "contents": "Cryosurgery for basal cell carcinoma of the head and neck. Thirty patients with 57 basal cell carcinomas of the head and neck underwent cryosurgery during the years 1971--1973. Treatment was performed in the outpatient clinic, and no premedication or local anaesthesia was required. Residual disease necessitating local excision was found in 4 patients at follow-up which extended over a period of 2 years. The technique proved to be simple and safe. However, since tissue was not available for histological control, rigid criteria for patient selection and a prolonged follow-up are required."}
{"id": "PMID:483101", "title": "The use of ultrasound in the diagnosis of pelvic masses.", "content": "Two hundred and two patients were referred to the ultrasound department for evaluation of a pelvic mass during 1977, and of these only 120 underwent an operation enabling a definitive diagnosis to be made. The diagnostic accuracy of ultrasound was compared with the final operative diagnosis. The great majority (98%) of pelvic masses could be detected, and ultrasound was accurate in predicting the antatomical site of a pelvic lesion in 88% of cases. Uterine lesions were precisely diagnosed in 87% of cases, but only 30% of adnexal conditions could be specifically recognized. Numerous situations in which ultrasound provided additional information of great clinical value were noted.", "contents": "The use of ultrasound in the diagnosis of pelvic masses. Two hundred and two patients were referred to the ultrasound department for evaluation of a pelvic mass during 1977, and of these only 120 underwent an operation enabling a definitive diagnosis to be made. The diagnostic accuracy of ultrasound was compared with the final operative diagnosis. The great majority (98%) of pelvic masses could be detected, and ultrasound was accurate in predicting the antatomical site of a pelvic lesion in 88% of cases. Uterine lesions were precisely diagnosed in 87% of cases, but only 30% of adnexal conditions could be specifically recognized. Numerous situations in which ultrasound provided additional information of great clinical value were noted."}
{"id": "PMID:483103", "title": "Endobronchial lipoma. A case report.", "content": "An endobronchial lipoma of the right main bronchus causing obstructive pneumonitis and bronchiectasis in a 71-year-old White man is described. Tumourlets were also found in the fibrosed lung. Although the tumour is rare, it is of clinical importance because it can be treated successfully by local excision.", "contents": "Endobronchial lipoma. A case report. An endobronchial lipoma of the right main bronchus causing obstructive pneumonitis and bronchiectasis in a 71-year-old White man is described. Tumourlets were also found in the fibrosed lung. Although the tumour is rare, it is of clinical importance because it can be treated successfully by local excision."}
{"id": "PMID:483109", "title": "Computed tomography of infratentorial masses in children.", "content": "Seventeen cases of infratentorial mass lesions (excluding abscesses) in children are reviewed. The findings after computed axial tomography (CAT) are described, as well as the changes that were revealed by plain radiographs of the skull. Ancillary investigations were performed in 11 cases, and their usefulness is assessed. The possibility of making a provisional diagnosis of the nature of the lesion based on CAT findings is discussed.", "contents": "Computed tomography of infratentorial masses in children. Seventeen cases of infratentorial mass lesions (excluding abscesses) in children are reviewed. The findings after computed axial tomography (CAT) are described, as well as the changes that were revealed by plain radiographs of the skull. Ancillary investigations were performed in 11 cases, and their usefulness is assessed. The possibility of making a provisional diagnosis of the nature of the lesion based on CAT findings is discussed."}
{"id": "PMID:483110", "title": "Displacement of the spinolaminar line--a sign of value in fractures of the upper cervical spine.", "content": "The spinolaminar line is an important anatomical landmark easily visualized on the lateral radiograph of the cervical spine. Any displacement in this line may be an indication of subtle traumatic vertebral damage. This is particularly relevant to the upper cervical spine in which the complex anatomy and frequent absence of associated neurological deficit make diagnosis difficult. Two case histories are presented which emphasize the importance of evaluation of the spinolaminar line on the lateral radiograph after trauma to the head and neck.", "contents": "Displacement of the spinolaminar line--a sign of value in fractures of the upper cervical spine. The spinolaminar line is an important anatomical landmark easily visualized on the lateral radiograph of the cervical spine. Any displacement in this line may be an indication of subtle traumatic vertebral damage. This is particularly relevant to the upper cervical spine in which the complex anatomy and frequent absence of associated neurological deficit make diagnosis difficult. Two case histories are presented which emphasize the importance of evaluation of the spinolaminar line on the lateral radiograph after trauma to the head and neck."}
{"id": "PMID:483111", "title": "A mannitol-bisacodyl regimen for radiological visualization of the colon.", "content": "The effectiveness of a 20% solution of mannitol-bisacodyl for oral use in preparing patients for barium enemas was investigated, in an investigator-blind control situation. The results were evaluated and compared with other methods currently in use. No comparative study where mannitol had been used could be found in the world literature. The mannitol-bisacodyl regimen was found to be an acceptable and reliable method for colon preparation in a high percentage of cases. Side-effects and changes in electrolyte balance of body fluids were recorded. The regimen was found to be safe for use on any patient irrespective of race, age or clinical condition and is not contraindicated in cases of cardiac and renal failure.", "contents": "A mannitol-bisacodyl regimen for radiological visualization of the colon. The effectiveness of a 20% solution of mannitol-bisacodyl for oral use in preparing patients for barium enemas was investigated, in an investigator-blind control situation. The results were evaluated and compared with other methods currently in use. No comparative study where mannitol had been used could be found in the world literature. The mannitol-bisacodyl regimen was found to be an acceptable and reliable method for colon preparation in a high percentage of cases. Side-effects and changes in electrolyte balance of body fluids were recorded. The regimen was found to be safe for use on any patient irrespective of race, age or clinical condition and is not contraindicated in cases of cardiac and renal failure."}
{"id": "PMID:483112", "title": "Diagnosis and treatment of abdominal aortic aneurysm. A report of 82 cases.", "content": "Resection for abdominal aortic aneurysm (AAA) is being performed with decreasing mortality and morbidity. A retrospective study on 82 consecutive patients with AAAs admitted during the period 1974-1977 shoowed that 66 had a non-ruptured aneurysm and 16 a ruptured aneurysm. The mean age of the patients was 66,7 years. Concomitant disease was frequent. Operative mortality aneurysms and 37% in the group with ruptured aneurysms.", "contents": "Diagnosis and treatment of abdominal aortic aneurysm. A report of 82 cases. Resection for abdominal aortic aneurysm (AAA) is being performed with decreasing mortality and morbidity. A retrospective study on 82 consecutive patients with AAAs admitted during the period 1974-1977 shoowed that 66 had a non-ruptured aneurysm and 16 a ruptured aneurysm. The mean age of the patients was 66,7 years. Concomitant disease was frequent. Operative mortality aneurysms and 37% in the group with ruptured aneurysms."}
{"id": "PMID:483113", "title": "An acquired inhibitor to factor XIII A case report.", "content": "An elderly woman presented with a postoperative bleeding problem, and a presumptive diagnosis of factor XIII (fibrin-stablilizing factor) deficiency was made. However, on further testing, an inhibitor to factor XIII was detected. She was on isoniazid (INH) therapy at the time. INH has been implicated in other patients who have developed inhibitors to factor XIII, and it was felt that this was the reason for this patient developing the inhibitor. The patient subsequently died from a cerebral haemorrhage, a well-described complication of factor XIII deficiency.", "contents": "An acquired inhibitor to factor XIII A case report. An elderly woman presented with a postoperative bleeding problem, and a presumptive diagnosis of factor XIII (fibrin-stablilizing factor) deficiency was made. However, on further testing, an inhibitor to factor XIII was detected. She was on isoniazid (INH) therapy at the time. INH has been implicated in other patients who have developed inhibitors to factor XIII, and it was felt that this was the reason for this patient developing the inhibitor. The patient subsequently died from a cerebral haemorrhage, a well-described complication of factor XIII deficiency."}
{"id": "PMID:483119", "title": "Men and women: changing roles and social security.", "content": "In the Social Security Amendments of 1977, Congress called for a study to examine ways to eliminate dependency as a factor in determining entitlement to spouse's benefits under the social security program as well as proposals to bring about the equal treatment of men and women. The report of the study undertaken in response to that change explores two options for making broad-scale changes--earnings sharing and the establishment of a double-decker benefit structure. The study was conducted by the Social Security Administration with assistance from the Department of Justice Task Force on Sex Discrimination and several other interested bodies. Public views were obtained from responses to the report of the HEW Task Force on the Treatment of Women Under Social Security and letters to the Advisory Council on Social Security. The Advisory Council is expected to make use of this report in its deliberations. The extensive excerpts that follow, which relate to the comprehensive options discussed, are taken verbatim from the summary of the report prepared by the Department of Health, Education and Welfare.", "contents": "Men and women: changing roles and social security. In the Social Security Amendments of 1977, Congress called for a study to examine ways to eliminate dependency as a factor in determining entitlement to spouse's benefits under the social security program as well as proposals to bring about the equal treatment of men and women. The report of the study undertaken in response to that change explores two options for making broad-scale changes--earnings sharing and the establishment of a double-decker benefit structure. The study was conducted by the Social Security Administration with assistance from the Department of Justice Task Force on Sex Discrimination and several other interested bodies. Public views were obtained from responses to the report of the HEW Task Force on the Treatment of Women Under Social Security and letters to the Advisory Council on Social Security. The Advisory Council is expected to make use of this report in its deliberations. The extensive excerpts that follow, which relate to the comprehensive options discussed, are taken verbatim from the summary of the report prepared by the Department of Health, Education and Welfare."}
{"id": "PMID:483121", "title": "Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism: isolation and characterization of a mutant deficient in the activity of phosphoribosylaminoimidazole synthetase.", "content": "A new purine-requiring mutant of Chinese hamster ovary cells (CHO-Kl) is described. This mutant, Ade-G, grows on aminoimidazole carboxamide, hypoxanthine, or adenine. It complements all eight of our other previously described Ade- mutants. Biochemical analysis of de novo purine synthesis in whole cells suggests that Ade-G is capable of the first four reactions of de novo purine biosynthesis and that it synthesizes and accumulates phosphoribosylformylglycinamidine (FGAM). Direct enzyme assay in cell-free extracts confirms that Ade-G is defective in phosphoribosylaminoimidazole synthetase activity and does not convert FGAM to phosphoribosylaminoimidazole (AIR), the next intermediate in the de novo biosynthetic pathway.", "contents": "Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism: isolation and characterization of a mutant deficient in the activity of phosphoribosylaminoimidazole synthetase. A new purine-requiring mutant of Chinese hamster ovary cells (CHO-Kl) is described. This mutant, Ade-G, grows on aminoimidazole carboxamide, hypoxanthine, or adenine. It complements all eight of our other previously described Ade- mutants. Biochemical analysis of de novo purine synthesis in whole cells suggests that Ade-G is capable of the first four reactions of de novo purine biosynthesis and that it synthesizes and accumulates phosphoribosylformylglycinamidine (FGAM). Direct enzyme assay in cell-free extracts confirms that Ade-G is defective in phosphoribosylaminoimidazole synthetase activity and does not convert FGAM to phosphoribosylaminoimidazole (AIR), the next intermediate in the de novo biosynthetic pathway."}
{"id": "PMID:483122", "title": "Respiration-deficient Chinese hamster cell mutants: genetic characterization.", "content": "We present here genetic experiments with a series of Chinese hamster cell mutants defective in oxidative energy metabolism. The mutations were all shown to be recessive in intraspecies hybrids. Thirty-five mutants were sorted into eight complementation groups, but one of these mutants failed to complement representatives of two distinct complementation groups. The possibility was raised that this is a cell carrying two mutations or a deletion. Because of the greatly different frequencies with which such mutants could be isolated from two different Chinese hamster cell lines, CCL16 (DON) and V79, the stability of representatives from each cell line was examined, and it was found that revertants could be obtained after treatment with mutagens, while spontaneous revertants appeared at unmeasurable or extremely low frequencies, with one exception. The mutant with a very noticeable frequency of spontaneous reversion was defective in mitochondrial protein synthesis, and the question arose whether the mutation was on the mitochondrial genome. A detailed fluctuation analysis of reversion rate and comparison with rates for other mutations was consistent with a nuclear mutation. This conclusion was supported by experiments involving fusions with cytoplasts.", "contents": "Respiration-deficient Chinese hamster cell mutants: genetic characterization. We present here genetic experiments with a series of Chinese hamster cell mutants defective in oxidative energy metabolism. The mutations were all shown to be recessive in intraspecies hybrids. Thirty-five mutants were sorted into eight complementation groups, but one of these mutants failed to complement representatives of two distinct complementation groups. The possibility was raised that this is a cell carrying two mutations or a deletion. Because of the greatly different frequencies with which such mutants could be isolated from two different Chinese hamster cell lines, CCL16 (DON) and V79, the stability of representatives from each cell line was examined, and it was found that revertants could be obtained after treatment with mutagens, while spontaneous revertants appeared at unmeasurable or extremely low frequencies, with one exception. The mutant with a very noticeable frequency of spontaneous reversion was defective in mitochondrial protein synthesis, and the question arose whether the mutation was on the mitochondrial genome. A detailed fluctuation analysis of reversion rate and comparison with rates for other mutations was consistent with a nuclear mutation. This conclusion was supported by experiments involving fusions with cytoplasts."}
{"id": "PMID:483123", "title": "Cytoplasmically inherited mutations of a human cell line resulting in deficient mitochondrial protein synthesis.", "content": "A large number of mutants deficient in mitochondrial protein synthesis (mtPS-) have been isolated from the human cell line VA2-B by subjecting cells partially depleted of their mtDNA to mutagenic treatments thought to be specific for mtDNA. Each of these mtPS- mutants has less than 10% of the wild-type rate of mitochondrial protein synthesis, exhibits reduced cytochrome oxidase and rutamycin sensitive ATPase activities, requires high concentrations of glucose, and grows indefinitely in the presence of 100 micrograms/ml of chloramphenicol (CAP). Fusion of cytoplasts from seven mtPS- mutants to the nucleated thioguanine-resistant VA2-B derivative TG-6 has yielded numerous cybrid clones which grow in CAP plus thioguanine, whereas almost no clones have resulted from the fusion of nucleated mtPS- cells to TG-6 cells: these results suggest that the gene(s) coding for the phenotype of mtPS- cells is localized in the cytoplasm (mtDNA?).", "contents": "Cytoplasmically inherited mutations of a human cell line resulting in deficient mitochondrial protein synthesis. A large number of mutants deficient in mitochondrial protein synthesis (mtPS-) have been isolated from the human cell line VA2-B by subjecting cells partially depleted of their mtDNA to mutagenic treatments thought to be specific for mtDNA. Each of these mtPS- mutants has less than 10% of the wild-type rate of mitochondrial protein synthesis, exhibits reduced cytochrome oxidase and rutamycin sensitive ATPase activities, requires high concentrations of glucose, and grows indefinitely in the presence of 100 micrograms/ml of chloramphenicol (CAP). Fusion of cytoplasts from seven mtPS- mutants to the nucleated thioguanine-resistant VA2-B derivative TG-6 has yielded numerous cybrid clones which grow in CAP plus thioguanine, whereas almost no clones have resulted from the fusion of nucleated mtPS- cells to TG-6 cells: these results suggest that the gene(s) coding for the phenotype of mtPS- cells is localized in the cytoplasm (mtDNA?)."}
{"id": "PMID:483124", "title": "Characterization of naturally occurring auxotrophic mammalian cells.", "content": "In a previous study, several cultured cell lines were detected which are naturally occurring auxotrophs. In this investigation, the enzyme deficienceis involved are described. It is demonstrated that the Chinese hamster cell lines CHO(K1), YH21, RJK-36, and CHW-1102 are deficient in cystathionase and argininosuccinate synthetase. In addition, CHO (K1) and CHW-1102 were found to lack argininosuccinate lyase. CHW-1102 cells were also found to be unable to proliferate in medium containing branched-chain alpha-keto acids in place of the corresponding L-amino acids since CHW-1102 cells lack branched-chain aminotransferase.", "contents": "Characterization of naturally occurring auxotrophic mammalian cells. In a previous study, several cultured cell lines were detected which are naturally occurring auxotrophs. In this investigation, the enzyme deficienceis involved are described. It is demonstrated that the Chinese hamster cell lines CHO(K1), YH21, RJK-36, and CHW-1102 are deficient in cystathionase and argininosuccinate synthetase. In addition, CHO (K1) and CHW-1102 were found to lack argininosuccinate lyase. CHW-1102 cells were also found to be unable to proliferate in medium containing branched-chain alpha-keto acids in place of the corresponding L-amino acids since CHW-1102 cells lack branched-chain aminotransferase."}
{"id": "PMID:483125", "title": "Tourniquet infusion chemotherapy in extremities with malignant lesions.", "content": "Tourniquet infusion chemotherapy involves the direct injection of a chemotherapeutic drug into the main artery of an extremity with prior application of an external tourniquet proximally on this extremity set at above the level of systolic pressure for ten minutes. Thus, the drug is not diluted and pushed by the blood into the venous circulation before diffusion into the tissues has occurred. This technique, applied in seven patients for a total of 40 instances, proved to be safe. Skin erythema and blisters, which are reversible, occur in the treated area. Complete clinical regression was achieved in all six patients with evaluable tumor, with high percentages of tumor necrosis. It was possible to avoid amputation in five of the seven patients treated. This technique appears superior to perfusion, but the long term permanence of regression has not yet been ascertained.", "contents": "Tourniquet infusion chemotherapy in extremities with malignant lesions. Tourniquet infusion chemotherapy involves the direct injection of a chemotherapeutic drug into the main artery of an extremity with prior application of an external tourniquet proximally on this extremity set at above the level of systolic pressure for ten minutes. Thus, the drug is not diluted and pushed by the blood into the venous circulation before diffusion into the tissues has occurred. This technique, applied in seven patients for a total of 40 instances, proved to be safe. Skin erythema and blisters, which are reversible, occur in the treated area. Complete clinical regression was achieved in all six patients with evaluable tumor, with high percentages of tumor necrosis. It was possible to avoid amputation in five of the seven patients treated. This technique appears superior to perfusion, but the long term permanence of regression has not yet been ascertained."}
{"id": "PMID:483126", "title": "Retroperitoneal cross pelvic bypass grafts.", "content": "An extraperitoneal retropubic cross iliac artery bypass graft is a safe alternative to an intra-abdominal aortoiliac bypass, axillofemoral bypass or subcutaneous femorofemoral bypass in the patient considered to be at high risk. This procedure may prove to be superior to subcutaneously placed grafts because the graft is less prone to trauma and infection. The circulation of the donor limb must be adequate to produce a significant increase in the rate of flow through the recipient limb. There was an early patency of the graft in 58 of 61 patients. There was patency of the graft at three years in 40 of 61 patients.", "contents": "Retroperitoneal cross pelvic bypass grafts. An extraperitoneal retropubic cross iliac artery bypass graft is a safe alternative to an intra-abdominal aortoiliac bypass, axillofemoral bypass or subcutaneous femorofemoral bypass in the patient considered to be at high risk. This procedure may prove to be superior to subcutaneously placed grafts because the graft is less prone to trauma and infection. The circulation of the donor limb must be adequate to produce a significant increase in the rate of flow through the recipient limb. There was an early patency of the graft in 58 of 61 patients. There was patency of the graft at three years in 40 of 61 patients."}
{"id": "PMID:483128", "title": "The value of bone scans in the management of patients with carcinoma of the breast.", "content": "The bone scan has become a major staging and screening test for patients with carcinoma of the breast. The routine use preoperatively of bone scans to detect occult osseous metastases has been advocated to prevent radical operations on patients with Stage IV disease. Periodic use post-operatively of bone scans has also been advocated to detect metastic disease before the onset of symptoms. In view of the prevalence of this disease, the implications of this approach in terms of cost alone warrant consideration. All bone scans obtained of patients with carcinoma of the breast during a five year period were reviewed and the results correlated with the pathologic stage of the disease, the presence or absence of symptoms and pertinent clinical data. The sensitivity, specificity and predictive value of the bond scan was then analyzed for each group. Recommendations for the use of bone scans in patients with carcinoma of the breast are based upon these data.", "contents": "The value of bone scans in the management of patients with carcinoma of the breast. The bone scan has become a major staging and screening test for patients with carcinoma of the breast. The routine use preoperatively of bone scans to detect occult osseous metastases has been advocated to prevent radical operations on patients with Stage IV disease. Periodic use post-operatively of bone scans has also been advocated to detect metastic disease before the onset of symptoms. In view of the prevalence of this disease, the implications of this approach in terms of cost alone warrant consideration. All bone scans obtained of patients with carcinoma of the breast during a five year period were reviewed and the results correlated with the pathologic stage of the disease, the presence or absence of symptoms and pertinent clinical data. The sensitivity, specificity and predictive value of the bond scan was then analyzed for each group. Recommendations for the use of bone scans in patients with carcinoma of the breast are based upon these data."}
{"id": "PMID:483129", "title": "Treatment of resistant lateral epicondylitis of the elbow by lengthening of the extensor carpi radialis brevis tendon.", "content": "Resistant lateral epicondylitis remains a difficult therapeutic problem. Lengthening of the extensor carpi radialis brevis has proved to be a simple, yet effective, method for treating this problem. Sixteen of the 18 patients in our series are asymptomatic and rated as having excellent results. The result in one patient is rated as good. The one failure in retrospect represented poor patient selection. The results we obtained support the original conclusions of another investigator that this simple surgical approach to the problem can give lasting relief. The procedure can be done on an ambulatory outpatient basis. Minimal postoperative restrictions are involved, and this procedure is appealing in terms of time of disability and cost.", "contents": "Treatment of resistant lateral epicondylitis of the elbow by lengthening of the extensor carpi radialis brevis tendon. Resistant lateral epicondylitis remains a difficult therapeutic problem. Lengthening of the extensor carpi radialis brevis has proved to be a simple, yet effective, method for treating this problem. Sixteen of the 18 patients in our series are asymptomatic and rated as having excellent results. The result in one patient is rated as good. The one failure in retrospect represented poor patient selection. The results we obtained support the original conclusions of another investigator that this simple surgical approach to the problem can give lasting relief. The procedure can be done on an ambulatory outpatient basis. Minimal postoperative restrictions are involved, and this procedure is appealing in terms of time of disability and cost."}
{"id": "PMID:483130", "title": "Ultrasonic detection and aspiration confirmation of intra-abdominal collection of fluid.", "content": "In patients clinically suspected of possessing intra-abdominal abscesses, ultrasonic imaging is an effective way of detecting suspicious localized fluid collections. However, definitive diagnosis depends upon sampling the fluid collections, which can be most easily performed by percutaneous fine needle aspiration of the collection under ultrasonic guidance. Sixty-six patients suspected of having intra-abdominal abscesses had localized fluid collections demonstrated by ultrasonic scanning. The composition of the fluid was proved in 40 patients by percutaneous aspiration; in 26, by surgical drainage alone, and in 30, by both methods. The aspiration procedure is relatively painless, rapid and free of complications.", "contents": "Ultrasonic detection and aspiration confirmation of intra-abdominal collection of fluid. In patients clinically suspected of possessing intra-abdominal abscesses, ultrasonic imaging is an effective way of detecting suspicious localized fluid collections. However, definitive diagnosis depends upon sampling the fluid collections, which can be most easily performed by percutaneous fine needle aspiration of the collection under ultrasonic guidance. Sixty-six patients suspected of having intra-abdominal abscesses had localized fluid collections demonstrated by ultrasonic scanning. The composition of the fluid was proved in 40 patients by percutaneous aspiration; in 26, by surgical drainage alone, and in 30, by both methods. The aspiration procedure is relatively painless, rapid and free of complications."}
{"id": "PMID:483131", "title": "Management of chronic atlantoaxial dislocation.", "content": "Twenty-two patients with chronic atlantoaxial dislocation have been treated. Of 13 patients with a reducible dislocation by skeletal traction, 12 underwent successful posterior infusion of the first, second and third cervical vertebrae. Of nine patients with irreducible dislocation, eight underwent surgical reduction and fusion through the transoral approach. The reduction was successful in all eight, but fusion was accomplished in four.", "contents": "Management of chronic atlantoaxial dislocation. Twenty-two patients with chronic atlantoaxial dislocation have been treated. Of 13 patients with a reducible dislocation by skeletal traction, 12 underwent successful posterior infusion of the first, second and third cervical vertebrae. Of nine patients with irreducible dislocation, eight underwent surgical reduction and fusion through the transoral approach. The reduction was successful in all eight, but fusion was accomplished in four."}
{"id": "PMID:483133", "title": "Vascular and orthopedic complications of knee dislocation.", "content": "Experience with complete dislocation of the knee in 22 consecutive patients during a six year period was analyzed. Major vascular complications occurred in nine of 13 extremities with anterior dislocation, one of seven extremities with posterior dislocation and none of two extremities with lateral dislocation. Liberal use of trans-femoral ateriography for diagnosis disclosed significant arterial injuries in four of 15 limbs, despite postreduction pedal pulses which were apparently normal. Limb salvage was accomplished in 20 of 21 survivors and in eight of nine with associated vascular complications. All patients demonstrated severe instability of the ligamentous structures of the knee consistent with the type of dislocation. Posterior instability was severe in all patients, an indication of disruption of the posterior cruciate ligament in every instance. Adequate follow-up information was available on 12 knees that had primary ligamentous repair, ten of which were stable to stress testing. Postoperative immobilization was accomplished by external skeletal fixation, skeletal traction or long leg posterior plaster splint.", "contents": "Vascular and orthopedic complications of knee dislocation. Experience with complete dislocation of the knee in 22 consecutive patients during a six year period was analyzed. Major vascular complications occurred in nine of 13 extremities with anterior dislocation, one of seven extremities with posterior dislocation and none of two extremities with lateral dislocation. Liberal use of trans-femoral ateriography for diagnosis disclosed significant arterial injuries in four of 15 limbs, despite postreduction pedal pulses which were apparently normal. Limb salvage was accomplished in 20 of 21 survivors and in eight of nine with associated vascular complications. All patients demonstrated severe instability of the ligamentous structures of the knee consistent with the type of dislocation. Posterior instability was severe in all patients, an indication of disruption of the posterior cruciate ligament in every instance. Adequate follow-up information was available on 12 knees that had primary ligamentous repair, ten of which were stable to stress testing. Postoperative immobilization was accomplished by external skeletal fixation, skeletal traction or long leg posterior plaster splint."}
{"id": "PMID:483134", "title": "Distal splenorenal shunt for decompression of portal hypertension in children with cystic fibrosis.", "content": "Apart from the sound physiologic basis for the distal splenorenal shunt as compared with the portacaval shunt and the conventional central splenorenal shunt, there are two important reasons why we think the use of this type of shunt is especially advantageous in children with portal hypertension secondary to cystic fibrosis. Firstly, the thick, fibrotic retroperitoneal area in the porta hepatis, where a portacaval shunt has to be constructed, can be avoided, which makes the distal splenorenal shunt the easier operation. Secondly, notwithstanding the relatively small-sized vessels, a wide anastomosis can be constructed with a high flow rate and, therefore, a minimal chance of shunt thrombosis.", "contents": "Distal splenorenal shunt for decompression of portal hypertension in children with cystic fibrosis. Apart from the sound physiologic basis for the distal splenorenal shunt as compared with the portacaval shunt and the conventional central splenorenal shunt, there are two important reasons why we think the use of this type of shunt is especially advantageous in children with portal hypertension secondary to cystic fibrosis. Firstly, the thick, fibrotic retroperitoneal area in the porta hepatis, where a portacaval shunt has to be constructed, can be avoided, which makes the distal splenorenal shunt the easier operation. Secondly, notwithstanding the relatively small-sized vessels, a wide anastomosis can be constructed with a high flow rate and, therefore, a minimal chance of shunt thrombosis."}
{"id": "PMID:483136", "title": "Risk of infection in prolonged central venous catheterization.", "content": "Classically, duration of infusion was thought to increase the rate of contamination by catheters. The results of this study show that this is not true and that, to the contrary, there is a definite difference in contamination rates between the basilic and the jugular and subclavian veins. The advantage of the basilic way which exists whatever the pathologic condition must undoubtedly determine the choice of this way of catheterization rather than any other.", "contents": "Risk of infection in prolonged central venous catheterization. Classically, duration of infusion was thought to increase the rate of contamination by catheters. The results of this study show that this is not true and that, to the contrary, there is a definite difference in contamination rates between the basilic and the jugular and subclavian veins. The advantage of the basilic way which exists whatever the pathologic condition must undoubtedly determine the choice of this way of catheterization rather than any other."}
{"id": "PMID:483137", "title": "Incidence of ischemic colitis following abdominal aortic reconstruction.", "content": "The incidence of ischemic colitis following abdominal aortic reconstruction was determined by postoperative colonoscopy during a three year period. The over-all incidence of colitis was 11 of 163 patients who underwent elective or urgent reconstruction of the abdominal aorta for aneurysmal and occlusive diseases. The incidence of ischemic colitis for patients who survived resection of ruptured abdominal aortic aneurysms was higher, although the colonoscopy rate was lower. The incidence might have been even higher if all patients surviving resection of ruptured abdominal aneurysms had undergone colonoscopy.", "contents": "Incidence of ischemic colitis following abdominal aortic reconstruction. The incidence of ischemic colitis following abdominal aortic reconstruction was determined by postoperative colonoscopy during a three year period. The over-all incidence of colitis was 11 of 163 patients who underwent elective or urgent reconstruction of the abdominal aorta for aneurysmal and occlusive diseases. The incidence of ischemic colitis for patients who survived resection of ruptured abdominal aortic aneurysms was higher, although the colonoscopy rate was lower. The incidence might have been even higher if all patients surviving resection of ruptured abdominal aneurysms had undergone colonoscopy."}
{"id": "PMID:483138", "title": "Scintiscans in the evaluation of patients with malignant melanomas.", "content": "In an attempt to define the role of scintiscanning in patients with a malignant melanoma, 349 liver, bone and brain scans in 136 patients have been reviewed. No patient with clinical Stage I or II disease was found to have occult metastases detected by scintiscans. All patients with proved positive scans demonstrated clinical symptoms consistent with the results of scintiscanning. Scintiscans in patients with a malignant melanoma are most effectively used to evaluate the symptomatic patient with Stage I or II disease and all patients with Stage III disease. Histologic verification of positive scans in patients with Stage I and II disease avoids unwarranted therapeutic decisions. There is, however, practical value in being able to predict accurately the absence of metastatic involvement. In only two of 249 examinations did the scans fail to detect metastases. A normal scan is a strong indicator of the absence of metastatic disease.", "contents": "Scintiscans in the evaluation of patients with malignant melanomas. In an attempt to define the role of scintiscanning in patients with a malignant melanoma, 349 liver, bone and brain scans in 136 patients have been reviewed. No patient with clinical Stage I or II disease was found to have occult metastases detected by scintiscans. All patients with proved positive scans demonstrated clinical symptoms consistent with the results of scintiscanning. Scintiscans in patients with a malignant melanoma are most effectively used to evaluate the symptomatic patient with Stage I or II disease and all patients with Stage III disease. Histologic verification of positive scans in patients with Stage I and II disease avoids unwarranted therapeutic decisions. There is, however, practical value in being able to predict accurately the absence of metastatic involvement. In only two of 249 examinations did the scans fail to detect metastases. A normal scan is a strong indicator of the absence of metastatic disease."}
{"id": "PMID:483143", "title": "Immobilization of fractures of the zygomatic bone with an antral pack.", "content": "Reduction and fixation of malar complex fractures has been emphasized and re-emphasized. Simple methods of reduction for complicated fractures are still advocated with clinically poor results. The necessity for a three point fixation and overcorrection for the complex fracture is emphasized. While it is true that simple fractures may be reduced by simple techniques, these simple methods cannot be applied to more comminuted fractures.", "contents": "Immobilization of fractures of the zygomatic bone with an antral pack. Reduction and fixation of malar complex fractures has been emphasized and re-emphasized. Simple methods of reduction for complicated fractures are still advocated with clinically poor results. The necessity for a three point fixation and overcorrection for the complex fracture is emphasized. While it is true that simple fractures may be reduced by simple techniques, these simple methods cannot be applied to more comminuted fractures."}
{"id": "PMID:483145", "title": "Results of early operations on cerebral aneurysms.", "content": "In 625 cases of cerebral aneurysms operated upon from 1971, those in which operation was performed within the first week after subarachnoid hemorrhage had the highest mortality. However, a closer analysis of these cases revealed that the results of surgical treatment were significantly different with each day. The mortality rates resulting from operation on the first and second days were low in comparison with the remaining days of the week. To evaluate the differences, the cases are presented and the best timing of surgery is discussed.", "contents": "Results of early operations on cerebral aneurysms. In 625 cases of cerebral aneurysms operated upon from 1971, those in which operation was performed within the first week after subarachnoid hemorrhage had the highest mortality. However, a closer analysis of these cases revealed that the results of surgical treatment were significantly different with each day. The mortality rates resulting from operation on the first and second days were low in comparison with the remaining days of the week. To evaluate the differences, the cases are presented and the best timing of surgery is discussed."}
{"id": "PMID:483146", "title": "A simplified method of CT assisted localization and biopsy of intracranial lesions.", "content": "A simplified technique available to most neurosurgeons is described for using computerized tomography (CT) to localize and/or biopsy intracranial lesions. Illustrative cases are presented.", "contents": "A simplified method of CT assisted localization and biopsy of intracranial lesions. A simplified technique available to most neurosurgeons is described for using computerized tomography (CT) to localize and/or biopsy intracranial lesions. Illustrative cases are presented."}
{"id": "PMID:483147", "title": "Epidural hematomas of the posterior fossa.", "content": "The authors report four cases of epidural hematoma of the posterior fossa. The presenting signs and symptoms are discussed. Emphasis is placed on the need for early recognition and treatment and the fact that concomitant supratentorial lesions may be present. The radiologic findings are discussed, and special note is made of the value of computed tomographic (CT) scanning. Suboccipital craniectomy may result in complete recovery, even in moribund cases, once the lision is suspected and treated.", "contents": "Epidural hematomas of the posterior fossa. The authors report four cases of epidural hematoma of the posterior fossa. The presenting signs and symptoms are discussed. Emphasis is placed on the need for early recognition and treatment and the fact that concomitant supratentorial lesions may be present. The radiologic findings are discussed, and special note is made of the value of computed tomographic (CT) scanning. Suboccipital craniectomy may result in complete recovery, even in moribund cases, once the lision is suspected and treated."}
{"id": "PMID:483148", "title": "Cryptococcal granuloma presenting as an intracranial mass.", "content": "A case of cryptococcosis of the central nervous system presenting as an intracranial space occupying lesion is described. The world literature and epidemiology of this unusual disease are surveyed. Problems in the diagnosis and the use of modern diagnostic techniques are discussed. The authors suggest that cryptococcosis should be included in the differential diagnosis of an intracranial space occupying lesion.", "contents": "Cryptococcal granuloma presenting as an intracranial mass. A case of cryptococcosis of the central nervous system presenting as an intracranial space occupying lesion is described. The world literature and epidemiology of this unusual disease are surveyed. Problems in the diagnosis and the use of modern diagnostic techniques are discussed. The authors suggest that cryptococcosis should be included in the differential diagnosis of an intracranial space occupying lesion."}
{"id": "PMID:483149", "title": "Intracerebral chondroma.", "content": "Intracerebral chondroma is an extremely rare condition. A case of a parasagittal intracerebral chondroma with no meningeal attachment is reported. The clinical and histological findings are described and a review of the literature is presented.", "contents": "Intracerebral chondroma. Intracerebral chondroma is an extremely rare condition. A case of a parasagittal intracerebral chondroma with no meningeal attachment is reported. The clinical and histological findings are described and a review of the literature is presented."}
{"id": "PMID:483150", "title": "Eosinophilic granuloma of the second cervical vertebra.", "content": "The occurrence of eosinophilic granuloma in the body of the second cervical vertebra of a young adult male is presented. The necessity of making a histologic diagnosis and prevention of vertebral instability led to operative intervention. Treatment consisted of posterior fixation of the spinous processes of the second and third cervical vertebrae with size 0 wire. Through a transoral approach the lesion was curetted, and bone chips were placed in the vertebral defect. The patient remains well, and disease-free, after 36 months.", "contents": "Eosinophilic granuloma of the second cervical vertebra. The occurrence of eosinophilic granuloma in the body of the second cervical vertebra of a young adult male is presented. The necessity of making a histologic diagnosis and prevention of vertebral instability led to operative intervention. Treatment consisted of posterior fixation of the spinous processes of the second and third cervical vertebrae with size 0 wire. Through a transoral approach the lesion was curetted, and bone chips were placed in the vertebral defect. The patient remains well, and disease-free, after 36 months."}
{"id": "PMID:483151", "title": "Thrombosis of the superior sagittal sinus associated with idiopathic thrombocytosis.", "content": "An unusual association of thrombosis of the superior sagittal sinus and idiopathic thrombocytosis is reported. Etiology, clinical and radiological findings and therapeutic approach are discussed.", "contents": "Thrombosis of the superior sagittal sinus associated with idiopathic thrombocytosis. An unusual association of thrombosis of the superior sagittal sinus and idiopathic thrombocytosis is reported. Etiology, clinical and radiological findings and therapeutic approach are discussed."}
{"id": "PMID:483152", "title": "The acute abdomen in patients with ventriculoperitoneal shunts.", "content": "A rationale for management of the patient with an acute adbomen and a ventriculoperitoneal shunt is presented in relation to eight patients. In two patients peritonitis was due to perforation of an abdominal viscus, not shunt related, and six were due to infections of ventriculoperitoneal shunts. Resolution of abdominal symptomatology occurs within six hours after the distal end of the shunt catheter is removed from the abdomen and placed in a drainage bottle. In four of these six, infection was limited to the peritoneal end of the catheter. The ventricular fluid was sterile.", "contents": "The acute abdomen in patients with ventriculoperitoneal shunts. A rationale for management of the patient with an acute adbomen and a ventriculoperitoneal shunt is presented in relation to eight patients. In two patients peritonitis was due to perforation of an abdominal viscus, not shunt related, and six were due to infections of ventriculoperitoneal shunts. Resolution of abdominal symptomatology occurs within six hours after the distal end of the shunt catheter is removed from the abdomen and placed in a drainage bottle. In four of these six, infection was limited to the peritoneal end of the catheter. The ventricular fluid was sterile."}
{"id": "PMID:483153", "title": "Intraperitoneal pseudocyst after ventriculoperitoneal shunt.", "content": "A case with intraperitoneal pseudocyst as a complication of a ventriculoperitoneal shunt is reported. The pathophysiology, diagnosis and treatment are discussed.", "contents": "Intraperitoneal pseudocyst after ventriculoperitoneal shunt. A case with intraperitoneal pseudocyst as a complication of a ventriculoperitoneal shunt is reported. The pathophysiology, diagnosis and treatment are discussed."}
{"id": "PMID:483154", "title": "Treatment of ruptured and unruptured internal carotid artery aneurysms.", "content": "A consecutive series of 95 patients with a total of 131 intracranial cerebral aneurysms came under observation from 1972-1978. Of these, 78 patients had operations for 106 aneurysms. Over half of the patients (41) had operations for an aneurysm of the intracranial internal carotid artery; there were 16 incidental \"asymptomatic\" aneurysms also found. There was no surgical mortality in this group. Medical treatment, including bed rest, antihypertensive treatment and administration of epsilon amino caproic acid followed by microsurgical obliteration of the aneurysm offers the best results for the treatment of ruptured and unruptured aneurysms.", "contents": "Treatment of ruptured and unruptured internal carotid artery aneurysms. A consecutive series of 95 patients with a total of 131 intracranial cerebral aneurysms came under observation from 1972-1978. Of these, 78 patients had operations for 106 aneurysms. Over half of the patients (41) had operations for an aneurysm of the intracranial internal carotid artery; there were 16 incidental \"asymptomatic\" aneurysms also found. There was no surgical mortality in this group. Medical treatment, including bed rest, antihypertensive treatment and administration of epsilon amino caproic acid followed by microsurgical obliteration of the aneurysm offers the best results for the treatment of ruptured and unruptured aneurysms."}
{"id": "PMID:483155", "title": "Malignant cardiac myxoma with cerebral metastases.", "content": "Two cases of cardiac myxoma with secondary involvement of the brain and death are reported. In one case, tumor emboli to the middle cerebral artery caused massive cerebral infarction, while in the other case true cerebral metastasis developed in the cerebrum and cerebellum, with rapid demise ensuing in both patients. It is emphasized that an emergency exists in cases ihat prompt echocardiography and computerized tomography of the brain are indicated. The second case represents the only instance of cardiac myxoma behaving as a true malignant neoplasm with actual invasion of the neural tissues recorded in the literature.", "contents": "Malignant cardiac myxoma with cerebral metastases. Two cases of cardiac myxoma with secondary involvement of the brain and death are reported. In one case, tumor emboli to the middle cerebral artery caused massive cerebral infarction, while in the other case true cerebral metastasis developed in the cerebrum and cerebellum, with rapid demise ensuing in both patients. It is emphasized that an emergency exists in cases ihat prompt echocardiography and computerized tomography of the brain are indicated. The second case represents the only instance of cardiac myxoma behaving as a true malignant neoplasm with actual invasion of the neural tissues recorded in the literature."}
{"id": "PMID:483156", "title": "The standardization of experimental impact injury to the spinal cord.", "content": "The biomechanical criteria for standardization of experimental impact injury to the spinal cord are derived. The theory is verified experimentally using cats. The undesirable effect due to heavy impounders is discussed. A falling mass of 20 gm and a small impounder 5 mm in diameter with a mass of 0.1 gm are recommended, leaving the momentum of the falling body to be controlled for different degrees of trauma.", "contents": "The standardization of experimental impact injury to the spinal cord. The biomechanical criteria for standardization of experimental impact injury to the spinal cord are derived. The theory is verified experimentally using cats. The undesirable effect due to heavy impounders is discussed. A falling mass of 20 gm and a small impounder 5 mm in diameter with a mass of 0.1 gm are recommended, leaving the momentum of the falling body to be controlled for different degrees of trauma."}
{"id": "PMID:483157", "title": "Facial myokymia.", "content": "Facial myokymia is an uncommon form of dyskinesia, usually resulting from a lesion in the brain stem. The electromyogram is typical. A case is reported. The condition was due to an astrocytoma of the brain stem.", "contents": "Facial myokymia. Facial myokymia is an uncommon form of dyskinesia, usually resulting from a lesion in the brain stem. The electromyogram is typical. A case is reported. The condition was due to an astrocytoma of the brain stem."}
{"id": "PMID:483161", "title": "Abdominal aortic coarctation and segmental hypoplasia.", "content": "Functionally significant, nonatherosclerotic, noninflammatory, concentric and tubular stenoses of the abdominal aorta, 4 to 16 cm in length, were encountered in five male and five female patients 11 to 49 years old. Seven patients were younger than 19 years of age. Aortic branch stenoses were common, affecting splanchnic vessels in seven patients and renal arteries in eight patients. The pathogenesis of the aortic constrictive lesions remains unknown, but it may be related to developmental error or aortic growth arrest. Existence of multiple renal arteries in 70% of these patients lends support to the developmental hypothesis. Intimal fibroplasia characterized stenotic aortic tissue. Severe hypertension was common, with the mean preoperative arterial pressure being 200/119 mm Hg. Thoracoabdominal bypass was undertaken in eight patients, being combined with renovascular reconstruction on five occasions. Two patients underwent patch graft aortoplasty with bilateral renal revascularization. Therapeutic results were classified as excellent six times and as good four times. Single-stage arterial reconstructions are the preferred method of treatment for abdominal aortic coarctation or segmental hypoplasia with associated aortic branch disease.", "contents": "Abdominal aortic coarctation and segmental hypoplasia. Functionally significant, nonatherosclerotic, noninflammatory, concentric and tubular stenoses of the abdominal aorta, 4 to 16 cm in length, were encountered in five male and five female patients 11 to 49 years old. Seven patients were younger than 19 years of age. Aortic branch stenoses were common, affecting splanchnic vessels in seven patients and renal arteries in eight patients. The pathogenesis of the aortic constrictive lesions remains unknown, but it may be related to developmental error or aortic growth arrest. Existence of multiple renal arteries in 70% of these patients lends support to the developmental hypothesis. Intimal fibroplasia characterized stenotic aortic tissue. Severe hypertension was common, with the mean preoperative arterial pressure being 200/119 mm Hg. Thoracoabdominal bypass was undertaken in eight patients, being combined with renovascular reconstruction on five occasions. Two patients underwent patch graft aortoplasty with bilateral renal revascularization. Therapeutic results were classified as excellent six times and as good four times. Single-stage arterial reconstructions are the preferred method of treatment for abdominal aortic coarctation or segmental hypoplasia with associated aortic branch disease."}
{"id": "PMID:483159", "title": "Progressive essential iris atrophy, Chandler's syndrome, and the iris nevus (Cogan-Reese) syndrome: a spectrum of disease.", "content": "Progressive essential iris atrophy, Chandler's syndrome, and the iris nevus (Cogan-Reese) syndrome are considered to be variations of a single disease process, which is characterized by abnormalities of the cornea, anterior chamber angle, and iris. In each variation, the typical patient is a white woman with unilateral disease, negative family history, and an onset of symptoms in early to middle adulthood. Since the membrane theory of Campbell suggests that the disease is a fundamental abnormality of the corneal endothelium, rather than the iris, the term \"iridocorneal endothelial syndrome,\" as proposed by Yanoff, may be an appropriate inclusive term for the spectrum of disease, although further study of the pathogenesis is needed. For each variation of the disease, corneal edema and secondary glucoma are both treated primarily by medical or surgical reduction of the intraocular pressure, although penetrating keratoplasty is occasionally required for cases with advanced corneal edema.", "contents": "Progressive essential iris atrophy, Chandler's syndrome, and the iris nevus (Cogan-Reese) syndrome: a spectrum of disease. Progressive essential iris atrophy, Chandler's syndrome, and the iris nevus (Cogan-Reese) syndrome are considered to be variations of a single disease process, which is characterized by abnormalities of the cornea, anterior chamber angle, and iris. In each variation, the typical patient is a white woman with unilateral disease, negative family history, and an onset of symptoms in early to middle adulthood. Since the membrane theory of Campbell suggests that the disease is a fundamental abnormality of the corneal endothelium, rather than the iris, the term \"iridocorneal endothelial syndrome,\" as proposed by Yanoff, may be an appropriate inclusive term for the spectrum of disease, although further study of the pathogenesis is needed. For each variation of the disease, corneal edema and secondary glucoma are both treated primarily by medical or surgical reduction of the intraocular pressure, although penetrating keratoplasty is occasionally required for cases with advanced corneal edema."}
{"id": "PMID:483163", "title": "Hepatic artery ligation.", "content": "The use of hepatic artery ligation (HAL) in various clinical situations is illustrated by presenting a series of eight patients. The indications for HAL included ruptured hepatic tumors, spontaneous liver rupture, delayed hemorrhage after liver trauma, hematobilia, hepatic artery aneurysm, and hemorrhage after liver biopsy. Conventional methods of hemostasis had been used in some of these patients, but failed to control hemorrhage. The reasons for the relatively late adoption of hepatic artery ligation in treating liver hemorrhage are discussed and placed in proper prospective. Hepatic artery ligation has been shown to be such an effective method of controlling hemorrhage from the liver that other methods, such as packing and mass suture, which are unsafe and ineffective, should be abandoned. Major resection should be done only when an entire lobe of the liver is reduced to pulp or when exposure and repair of the retrohepatic vena cava are necessary.", "contents": "Hepatic artery ligation. The use of hepatic artery ligation (HAL) in various clinical situations is illustrated by presenting a series of eight patients. The indications for HAL included ruptured hepatic tumors, spontaneous liver rupture, delayed hemorrhage after liver trauma, hematobilia, hepatic artery aneurysm, and hemorrhage after liver biopsy. Conventional methods of hemostasis had been used in some of these patients, but failed to control hemorrhage. The reasons for the relatively late adoption of hepatic artery ligation in treating liver hemorrhage are discussed and placed in proper prospective. Hepatic artery ligation has been shown to be such an effective method of controlling hemorrhage from the liver that other methods, such as packing and mass suture, which are unsafe and ineffective, should be abandoned. Major resection should be done only when an entire lobe of the liver is reduced to pulp or when exposure and repair of the retrohepatic vena cava are necessary."}
{"id": "PMID:483165", "title": "Peritoneal mesotheliomas.", "content": "Eight cases of peritoneal mesothelioma have been seen at the University of Missouri Medical Center and affiliated hospitals since 1940. The incidence for our patient population is 0.24 cases per 100,000 patients. The nonspecific symptoms, physical findings, x-rays, and laboratory results usually preclude an early establishment of the diagnosis. The diagnosis was eventually established at laparotomy in seven cases and at autopsy in one. Only the benign mesothelioma was resectable; that patient is doing well 10 months after operation. The survival interval of the patients with malignant peritoneal mesotheliomas averaged 3 months, with the two patients who received chemotherapy surviving 3 and 6 months, respectively.", "contents": "Peritoneal mesotheliomas. Eight cases of peritoneal mesothelioma have been seen at the University of Missouri Medical Center and affiliated hospitals since 1940. The incidence for our patient population is 0.24 cases per 100,000 patients. The nonspecific symptoms, physical findings, x-rays, and laboratory results usually preclude an early establishment of the diagnosis. The diagnosis was eventually established at laparotomy in seven cases and at autopsy in one. Only the benign mesothelioma was resectable; that patient is doing well 10 months after operation. The survival interval of the patients with malignant peritoneal mesotheliomas averaged 3 months, with the two patients who received chemotherapy surviving 3 and 6 months, respectively."}
{"id": "PMID:483160", "title": "On understanding the optics of intraocular lenses.", "content": "The optical considerations of intraocular lenses can be understood, using only the formulae and principles taught in a typical basic science course in ophthalmology. Some of the more common optical problems associated with intraocular lenses are presented and the methods for their solutions are illustrated and explained.", "contents": "On understanding the optics of intraocular lenses. The optical considerations of intraocular lenses can be understood, using only the formulae and principles taught in a typical basic science course in ophthalmology. Some of the more common optical problems associated with intraocular lenses are presented and the methods for their solutions are illustrated and explained."}
{"id": "PMID:483166", "title": "Correction of hypersplenism following distal splenorenal shunt.", "content": "The effect of splenorenal shunt on hypersplenism was assessed in 47 patients with splenomegaly, 26 of whom had significant thrombocytopenia or leukopenia. Of 16 patients with thrombocytopenia, platelet count returned to normal in 15 (94%) following operation, an improvement which was statistically highly significant (P less than 0.001). Of 16 patients with leukopenia, leukocyte count returned to normal in 11 (69%), also a highly significant improvement (P less than 0.001). Dramatic relief of hypersplenism occurs in the majority of patients following splenorenal shunt. Thrombocytopenia is more consistently corrected than is leukopenia. The etiology of liver disease appeared not to be a factor, but leukopenia was corrected more consistently in alcoholic than in nonalcoholic patients, while there was no difference in the postoperative response of thrombocytopenia to the operation. Long-term follow-up in 26 patients demonstrated sustained improvement in 57% of patients with preoperative leukopenia and 78% of patients with thrombocytopenia. Since significant improvement in leukopenia and thrombocytopenia will occur following the distal splenorenal shunt, hypersplenism is not a contraindication to this procedure.", "contents": "Correction of hypersplenism following distal splenorenal shunt. The effect of splenorenal shunt on hypersplenism was assessed in 47 patients with splenomegaly, 26 of whom had significant thrombocytopenia or leukopenia. Of 16 patients with thrombocytopenia, platelet count returned to normal in 15 (94%) following operation, an improvement which was statistically highly significant (P less than 0.001). Of 16 patients with leukopenia, leukocyte count returned to normal in 11 (69%), also a highly significant improvement (P less than 0.001). Dramatic relief of hypersplenism occurs in the majority of patients following splenorenal shunt. Thrombocytopenia is more consistently corrected than is leukopenia. The etiology of liver disease appeared not to be a factor, but leukopenia was corrected more consistently in alcoholic than in nonalcoholic patients, while there was no difference in the postoperative response of thrombocytopenia to the operation. Long-term follow-up in 26 patients demonstrated sustained improvement in 57% of patients with preoperative leukopenia and 78% of patients with thrombocytopenia. Since significant improvement in leukopenia and thrombocytopenia will occur following the distal splenorenal shunt, hypersplenism is not a contraindication to this procedure."}
{"id": "PMID:483170", "title": "Cimetidine affords protection equal to antacids in prevention of stress ulceration following thermal injury.", "content": "A prospective, randomized, double-blind, endoscopic study is described in which the efficacy of cimetidine (13 patients) is compared to that of antacids (14 patients) in the prevention of stress ulceration following severe thermal injury. Each treatment modality was equally effective in the prevention of acute gastroduodenal disease and its associated complications. Both cimetidine and antacids produced a near elimination of duodenal disease and markedly reduced the severity of gastric disease when compared to that of untreated historical controls. Lack of major side effects and ease of administration make cimetidine an attractive alternative to antacid therapy in the prophylaxis of stress-induced gastroduodenal disease in the thermally injured patient.", "contents": "Cimetidine affords protection equal to antacids in prevention of stress ulceration following thermal injury. A prospective, randomized, double-blind, endoscopic study is described in which the efficacy of cimetidine (13 patients) is compared to that of antacids (14 patients) in the prevention of stress ulceration following severe thermal injury. Each treatment modality was equally effective in the prevention of acute gastroduodenal disease and its associated complications. Both cimetidine and antacids produced a near elimination of duodenal disease and markedly reduced the severity of gastric disease when compared to that of untreated historical controls. Lack of major side effects and ease of administration make cimetidine an attractive alternative to antacid therapy in the prophylaxis of stress-induced gastroduodenal disease in the thermally injured patient."}
{"id": "PMID:483172", "title": "Duodenal perforation in blunt abdominal trauma.", "content": "Ten patients with duodenal perforation secondary to blunt abdominal trauma are reviewed. All 10 patients survived. Most of the injuries were related to motor vehicle accidents. Early recognition requires a high index of suspicion because preoperative signs and symptoms tend to be nonspecific and x-rays are usually not helpful. A careful exploration of the duodenum at laparotomy including both the Kocher maneuver and the Cattell-Braasch exposure is essential. Suitable methods of surgical repair are numerous, but those providing for restoration of normal gastrointestinal tract continuity are preferred.", "contents": "Duodenal perforation in blunt abdominal trauma. Ten patients with duodenal perforation secondary to blunt abdominal trauma are reviewed. All 10 patients survived. Most of the injuries were related to motor vehicle accidents. Early recognition requires a high index of suspicion because preoperative signs and symptoms tend to be nonspecific and x-rays are usually not helpful. A careful exploration of the duodenum at laparotomy including both the Kocher maneuver and the Cattell-Braasch exposure is essential. Suitable methods of surgical repair are numerous, but those providing for restoration of normal gastrointestinal tract continuity are preferred."}
{"id": "PMID:483173", "title": "Endoscopic retrograde pancreatography in the surgery of pancreatic pseudocysts.", "content": "Endoscopic retrograde pancreatography (ERP) was performed on 83 patients with 99 pancreatic pseudocysts (PCP). ERP was successful in 76 patients; in the other seven PCPs were found at operation. Five characteristic ductal patterns are described and illustrated. The PCPs were situated in the head (51 patients), body (21 patients), tail (20 patients). Thirteen PCPs were less than 2 cm, 50 were between 2 and 5 cm, 29 greater than 5 cm. There were multiple PCPs in 13.2% of patients. ERP was a more accurate diagnostic measure than ultrasonography or upper gastrointestinal roentgenograms, revealing the lesion in 89% of patients. Eight patients with pancreatic ascites had the causative PCP demonstrated prior to operation. Three patients had pancreatic pleural effusions; two with effusions on the right had PCPs of the tail. Five patients had spontaneous connections between a PCP and the stomach (four patients) or duodenum (one patient). Rational surgical planning for the management of PCPs is greatly facilitated by preliminary reconnaisance by ERP.", "contents": "Endoscopic retrograde pancreatography in the surgery of pancreatic pseudocysts. Endoscopic retrograde pancreatography (ERP) was performed on 83 patients with 99 pancreatic pseudocysts (PCP). ERP was successful in 76 patients; in the other seven PCPs were found at operation. Five characteristic ductal patterns are described and illustrated. The PCPs were situated in the head (51 patients), body (21 patients), tail (20 patients). Thirteen PCPs were less than 2 cm, 50 were between 2 and 5 cm, 29 greater than 5 cm. There were multiple PCPs in 13.2% of patients. ERP was a more accurate diagnostic measure than ultrasonography or upper gastrointestinal roentgenograms, revealing the lesion in 89% of patients. Eight patients with pancreatic ascites had the causative PCP demonstrated prior to operation. Three patients had pancreatic pleural effusions; two with effusions on the right had PCPs of the tail. Five patients had spontaneous connections between a PCP and the stomach (four patients) or duodenum (one patient). Rational surgical planning for the management of PCPs is greatly facilitated by preliminary reconnaisance by ERP."}
{"id": "PMID:483175", "title": "Necrotizing anorectal and perineal infections.", "content": "Sixteen cases of spontaneous necrotizing infection of the anorectum and perineum are described. Thirteen patients had clostridial infections and three had infections with nonclostridial organisms. Six patients were diabetic and two had leukemia. All 16 patients presented with pain, tenderness, swelling, and crepitation. Four had an ominous black spot on the scrotum or posterior labia. Shortly after initial recognition by the patient, all infections rapidly disseminated to include all surrounding areas such as the external genitalia, the anterior abdominal wall, and thighs. Treatment consisted of radical debridement and antibiotics; hyperbaric oxygen was used in the clostridial cases. Ten of the 16 patients survived. Delayed diagnosis and delayed treatment were the primary factors responsible for death.", "contents": "Necrotizing anorectal and perineal infections. Sixteen cases of spontaneous necrotizing infection of the anorectum and perineum are described. Thirteen patients had clostridial infections and three had infections with nonclostridial organisms. Six patients were diabetic and two had leukemia. All 16 patients presented with pain, tenderness, swelling, and crepitation. Four had an ominous black spot on the scrotum or posterior labia. Shortly after initial recognition by the patient, all infections rapidly disseminated to include all surrounding areas such as the external genitalia, the anterior abdominal wall, and thighs. Treatment consisted of radical debridement and antibiotics; hyperbaric oxygen was used in the clostridial cases. Ten of the 16 patients survived. Delayed diagnosis and delayed treatment were the primary factors responsible for death."}
{"id": "PMID:483182", "title": "Further clinical observations on the pulmonary effects of paraquat ingestion.", "content": "Five patients are reported who developed evidence of acute lung damage after proved ingestion of paraquat. In two the lung changes resolved; in one an aspiration pneumonia occurred, which was successfully treated, while two developed fatal pulmonary oedema. These pulmonary complications after paraquat intoxication appear more common than the progressive pulmonary fibrosis previously described. It is suggested that acute pulmonary oedema is a response to large doses, usually of Gramoxone, that subclinical lung changes result from small doses, usually of Weedol, and that pulmonary fibrosis occurs after intermediate doses. Preliminary data on plasma paraquat concentrations suggest that these are of value in prognosis.", "contents": "Further clinical observations on the pulmonary effects of paraquat ingestion. Five patients are reported who developed evidence of acute lung damage after proved ingestion of paraquat. In two the lung changes resolved; in one an aspiration pneumonia occurred, which was successfully treated, while two developed fatal pulmonary oedema. These pulmonary complications after paraquat intoxication appear more common than the progressive pulmonary fibrosis previously described. It is suggested that acute pulmonary oedema is a response to large doses, usually of Gramoxone, that subclinical lung changes result from small doses, usually of Weedol, and that pulmonary fibrosis occurs after intermediate doses. Preliminary data on plasma paraquat concentrations suggest that these are of value in prognosis."}
{"id": "PMID:483183", "title": "Effective time of the forced expiratory spirogram in health and airways obstruction.", "content": "We have calculated the effective time (t eff) of the forced expiratory spirogram in healthy non-smokers, in patients with simple chronic bronchitis, and in patients with chronic obstructive lung disease. Effective time was obtained by dividing the area underneath the forced expiratory spirogram by the forced vital capacity (FVC) and is equal to the time constant of lung emptying if the FVC-time curve is a single exponential. In 75 healthy non-smoking adults, t eff showed a linear increase with age but no difference between the sexes. In 37 patients with low forced expiratory volume in one second (FEV1)/FVC ratios, t eff, as expected, was increased. In 19 subjects with simple chronic bronchitis and normal FEV1/FVC ratios, t eff was longer than or at the upper limit of 2 SE above the mean for healthy subjects of the same age. A comparison of t eff with FEV1/FVC suggested that in most normal subjects and patients the forced expiratory spirogram was a multiple exponential and that t eff was a more sensitive index for the evaluation of airway obstruction than the FEV1/FVC ratio.", "contents": "Effective time of the forced expiratory spirogram in health and airways obstruction. We have calculated the effective time (t eff) of the forced expiratory spirogram in healthy non-smokers, in patients with simple chronic bronchitis, and in patients with chronic obstructive lung disease. Effective time was obtained by dividing the area underneath the forced expiratory spirogram by the forced vital capacity (FVC) and is equal to the time constant of lung emptying if the FVC-time curve is a single exponential. In 75 healthy non-smoking adults, t eff showed a linear increase with age but no difference between the sexes. In 37 patients with low forced expiratory volume in one second (FEV1)/FVC ratios, t eff, as expected, was increased. In 19 subjects with simple chronic bronchitis and normal FEV1/FVC ratios, t eff was longer than or at the upper limit of 2 SE above the mean for healthy subjects of the same age. A comparison of t eff with FEV1/FVC suggested that in most normal subjects and patients the forced expiratory spirogram was a multiple exponential and that t eff was a more sensitive index for the evaluation of airway obstruction than the FEV1/FVC ratio."}
{"id": "PMID:483184", "title": "Transit-time analysis of the forced expiratory spirogram during clinical remission in juvenile asthma.", "content": "The mean transit time (MTT), coefficient of variation (CoV), and index of skewness (IoS) of transit times of the forced expiratory flow/time curve have been compared with maximal mid-expiratory flow (MMEF), flow at 75, 50, and 25% of vital capacity (V75, V50, and V25), and the ratio of forced expiratory volume in 1 second (FEV1) to vital capacity (VC) in 51 asthmatic children judged on clinical grounds to be in remission. In 19 children all eight indices were normal. Of the remaining 32, MTT was normal in 5, V50 in 11, V75 in 12, and FEV1/VC and MMEF in 13 each. MTT was significantly more sensitive in detecting abnormality when other indices were normal, and abnormalities in MTT were of significantly greater magnitude when present. In 12 patients with at least one abnormal index given salbutamol aerosol, MTT was not more sensitive than MMEF, V25, FEV1/VC, or V50 in detecting change.", "contents": "Transit-time analysis of the forced expiratory spirogram during clinical remission in juvenile asthma. The mean transit time (MTT), coefficient of variation (CoV), and index of skewness (IoS) of transit times of the forced expiratory flow/time curve have been compared with maximal mid-expiratory flow (MMEF), flow at 75, 50, and 25% of vital capacity (V75, V50, and V25), and the ratio of forced expiratory volume in 1 second (FEV1) to vital capacity (VC) in 51 asthmatic children judged on clinical grounds to be in remission. In 19 children all eight indices were normal. Of the remaining 32, MTT was normal in 5, V50 in 11, V75 in 12, and FEV1/VC and MMEF in 13 each. MTT was significantly more sensitive in detecting abnormality when other indices were normal, and abnormalities in MTT were of significantly greater magnitude when present. In 12 patients with at least one abnormal index given salbutamol aerosol, MTT was not more sensitive than MMEF, V25, FEV1/VC, or V50 in detecting change."}
{"id": "PMID:483185", "title": "The pulmonary endothelial cell.", "content": "The surface of the endothelial cells of the pulmonary trunk of the Wistar albino rat was studied by means of silver preparations and by scanning and transmission electron microscopy. This surface is the site of cytoplasmic projections and the opening of caveolae which together appear to be features associated with the active metabolic r\u00f4le of the pulmonary endothelial cell.", "contents": "The pulmonary endothelial cell. The surface of the endothelial cells of the pulmonary trunk of the Wistar albino rat was studied by means of silver preparations and by scanning and transmission electron microscopy. This surface is the site of cytoplasmic projections and the opening of caveolae which together appear to be features associated with the active metabolic r\u00f4le of the pulmonary endothelial cell."}
{"id": "PMID:483186", "title": "Binding and ingestion of human lactoferrin by mouse alveolar macrophages.", "content": "59Fe-labelled human lactoferrin was found to be preferentially ingested by mouse alveolar macrophages (MAM) when compared to 59Fe-labelled human transferrin. The cells bound and ingested 125I-labelled iron-saturated and iron-free lactoferrin. The latter was digested faster (t1/2 = 5.8 hours), however, than the iron-saturated compound (t1/2 = 10.5 hours). The constant elimination of the Fe-lactoferrin complex by alveolar macrophages could enhance the bacteriostatic effect of lactoferrin in the pulmonary secretions.", "contents": "Binding and ingestion of human lactoferrin by mouse alveolar macrophages. 59Fe-labelled human lactoferrin was found to be preferentially ingested by mouse alveolar macrophages (MAM) when compared to 59Fe-labelled human transferrin. The cells bound and ingested 125I-labelled iron-saturated and iron-free lactoferrin. The latter was digested faster (t1/2 = 5.8 hours), however, than the iron-saturated compound (t1/2 = 10.5 hours). The constant elimination of the Fe-lactoferrin complex by alveolar macrophages could enhance the bacteriostatic effect of lactoferrin in the pulmonary secretions."}
{"id": "PMID:483187", "title": "Hypoxaemia in chronic obstructive bronchitis.", "content": "Arterial blood gas tensions were studied for six years in 85 patients (59 men, 26 women, mean age 58.8 years) with hypoxaemia associated with chronic bronchitis. All patients who died had a precipitous fall of arterial oxygen tension (PaO2) breathing air. In patients dying within two years of the first appearance of ankle oedema the mean rate of fall of PaO2 was 0.11 kPa/month. Patients who survived two years appeared to deteriorate more slowly (0.017 kPa/month) until some months before death, when they too deteriorated rapidly. Hypoxaemic patients with obstructive airways disease suffer a terminal rapid decline in arterial oxygen tension, which probably indicates real pathological change in the lungs and has important implications for long-term domiciliary oxygen treatment.", "contents": "Hypoxaemia in chronic obstructive bronchitis. Arterial blood gas tensions were studied for six years in 85 patients (59 men, 26 women, mean age 58.8 years) with hypoxaemia associated with chronic bronchitis. All patients who died had a precipitous fall of arterial oxygen tension (PaO2) breathing air. In patients dying within two years of the first appearance of ankle oedema the mean rate of fall of PaO2 was 0.11 kPa/month. Patients who survived two years appeared to deteriorate more slowly (0.017 kPa/month) until some months before death, when they too deteriorated rapidly. Hypoxaemic patients with obstructive airways disease suffer a terminal rapid decline in arterial oxygen tension, which probably indicates real pathological change in the lungs and has important implications for long-term domiciliary oxygen treatment."}
{"id": "PMID:483189", "title": "Serial pulmonary function tests in progressive systemic sclerosis.", "content": "Serial pulmonary function tests were performed on nine patients with progressive systemic sclerosis over a mean period of ten years. Abnormality was seen to develop both early and late in the course of the disease, and the earliest abnormality observed was impairment of the transfer factor. Deterioration of some aspect of pulmonary function was noted in each case, evidence of restriction or air trapping being seen with equal frequency. Two patients died, both of causes unrelated to their pulmonary involvement, and even pronounced early involvement of the lungs did not necessarily imply a bad prognosis.", "contents": "Serial pulmonary function tests in progressive systemic sclerosis. Serial pulmonary function tests were performed on nine patients with progressive systemic sclerosis over a mean period of ten years. Abnormality was seen to develop both early and late in the course of the disease, and the earliest abnormality observed was impairment of the transfer factor. Deterioration of some aspect of pulmonary function was noted in each case, evidence of restriction or air trapping being seen with equal frequency. Two patients died, both of causes unrelated to their pulmonary involvement, and even pronounced early involvement of the lungs did not necessarily imply a bad prognosis."}
{"id": "PMID:483190", "title": "Carcinoma of the lung in Lancashire coalminers.", "content": "The prevalence at death of carcinoma of the lung in miners and ex-miners has been compared in those with and without pneumoconiosis at necropsy. The prevalence of 11.4% in the group as a whole is no greater than that in the male population in North-west England. Carcinoma of the lung was present in 62 (13.1%) of those without pneumoconiosis and in 52 (9.8%) of those with pneumoconiosis. The mean age at death of those with pneumoconiosis was 71.3 years so that they cannot be said to have died before the age at which they would have developed carcinoma. Those with progressive massive fibrosis whose mean age at death was 72 years had the lowest prevalence of carcinoma of the lung at all ages--8.4%. For reasons stated in the text this is inevitably a biased sample. The number of those without pneumoconiosis is probably lower than the true figure because the deaths of miners and ex-miners in whom there is no suspicion of lung disease may not have been reported to the coroner or to the pneumoconiosis medical panel. There appears to be no positive link between carcinoma of the lung and pneumoconiosis. There is a surprisingly high number of smokers and ex-smokers among these miners, and this appears to have more relevance to the prevalence of carcinoma of the lung than does pneumoconiosis.", "contents": "Carcinoma of the lung in Lancashire coalminers. The prevalence at death of carcinoma of the lung in miners and ex-miners has been compared in those with and without pneumoconiosis at necropsy. The prevalence of 11.4% in the group as a whole is no greater than that in the male population in North-west England. Carcinoma of the lung was present in 62 (13.1%) of those without pneumoconiosis and in 52 (9.8%) of those with pneumoconiosis. The mean age at death of those with pneumoconiosis was 71.3 years so that they cannot be said to have died before the age at which they would have developed carcinoma. Those with progressive massive fibrosis whose mean age at death was 72 years had the lowest prevalence of carcinoma of the lung at all ages--8.4%. For reasons stated in the text this is inevitably a biased sample. The number of those without pneumoconiosis is probably lower than the true figure because the deaths of miners and ex-miners in whom there is no suspicion of lung disease may not have been reported to the coroner or to the pneumoconiosis medical panel. There appears to be no positive link between carcinoma of the lung and pneumoconiosis. There is a surprisingly high number of smokers and ex-smokers among these miners, and this appears to have more relevance to the prevalence of carcinoma of the lung than does pneumoconiosis."}
{"id": "PMID:483191", "title": "The pulmonary angiographic appearance of pleurisy associated with subdiaphragmatic inflammation.", "content": "In two patients who had recently undergone major abdominal operations and were later suspected of having pulmonary emboli, pulmonary angiography showed no evidence of embolism, but in both cases one of the hemidiaphragms was clearly outlined by contrast material. It is suggested that subdiaphragmatic inflammation was responsible for this unusual appearance.", "contents": "The pulmonary angiographic appearance of pleurisy associated with subdiaphragmatic inflammation. In two patients who had recently undergone major abdominal operations and were later suspected of having pulmonary emboli, pulmonary angiography showed no evidence of embolism, but in both cases one of the hemidiaphragms was clearly outlined by contrast material. It is suggested that subdiaphragmatic inflammation was responsible for this unusual appearance."}
{"id": "PMID:483192", "title": "Melanotic spinothoracic schwannoma.", "content": "A dumb-bell mediastinal melanotic schwannoma is described, and the rarity of this type of tumour emphasised. The tumour was resected by simultaneous laminectomy and posterior thoracotomy. The patient is well four years after operation. The pathology of the tumour is described and the origin of melanotic cells is discussed.", "contents": "Melanotic spinothoracic schwannoma. A dumb-bell mediastinal melanotic schwannoma is described, and the rarity of this type of tumour emphasised. The tumour was resected by simultaneous laminectomy and posterior thoracotomy. The patient is well four years after operation. The pathology of the tumour is described and the origin of melanotic cells is discussed."}
{"id": "PMID:483193", "title": "Technique for the management of anterior flail chest.", "content": "A technique for the management of anterior flail chest consisting of osteosynthesis and the positioning of two long Kirschner wires behind the sternum in the form of a St Andrew's cross is described. The procedure is easy to perform, the patient is ambulant early, and the results are good.", "contents": "Technique for the management of anterior flail chest. A technique for the management of anterior flail chest consisting of osteosynthesis and the positioning of two long Kirschner wires behind the sternum in the form of a St Andrew's cross is described. The procedure is easy to perform, the patient is ambulant early, and the results are good."}
{"id": "PMID:483194", "title": "Cost of a cardiac surgical and a general thoracic surgical patient to the National Health Service in a London teaching hospital.", "content": "The cost of the inpatient stay for a typical aortic valve replacement and for an oesophagectomy were determined by recording and costing every aspect of the patients' care from admission until discharge. This method of cost calculation was found to be satisfactory and could be used by other centres to allow comparisons between hospitals or countries. At St Thomas's Hospital in 1977 the cost of a cardiac operation was 2755 pounds, an oesophagectomy 1870 pounds, and a general surgical operation 564 pounds.", "contents": "Cost of a cardiac surgical and a general thoracic surgical patient to the National Health Service in a London teaching hospital. The cost of the inpatient stay for a typical aortic valve replacement and for an oesophagectomy were determined by recording and costing every aspect of the patients' care from admission until discharge. This method of cost calculation was found to be satisfactory and could be used by other centres to allow comparisons between hospitals or countries. At St Thomas's Hospital in 1977 the cost of a cardiac operation was 2755 pounds, an oesophagectomy 1870 pounds, and a general surgical operation 564 pounds."}
{"id": "PMID:483195", "title": "Assessment of respiratory function in patients with chronic obstructive airways disease.", "content": "The reproducibility of some respiratory function tests and a simple self-paced walking test were assessed in 13 patients with stable chronic obstructive airways disease by making observations on six occasions at two to three weekly intervals. The coefficients of variation of the various tests were as follows: forced expiratory volume in one second, +/- 14.8%; forced vital capacity, +/- 11.1%; transfer factor (single breath carbon monoxide), +/- 15%; total lung capacity, +/- 8.8% by helium dilution and +/- 8.3% by body plethysmography; increase in heart rate and minute ventilation for an increase in oxygen uptake of 0.5 1 min-1, +/- 19.4% and +/- 16.3%; distance covered in a 12-minute walking test, +/- 8.2%. The 12-minute distance increased significantly over the first three studies. The considerable variations in the result of these tests in subjects with apparently stable clinical states and without changes in treatment should be considered when assessing the results of changes in treatment.", "contents": "Assessment of respiratory function in patients with chronic obstructive airways disease. The reproducibility of some respiratory function tests and a simple self-paced walking test were assessed in 13 patients with stable chronic obstructive airways disease by making observations on six occasions at two to three weekly intervals. The coefficients of variation of the various tests were as follows: forced expiratory volume in one second, +/- 14.8%; forced vital capacity, +/- 11.1%; transfer factor (single breath carbon monoxide), +/- 15%; total lung capacity, +/- 8.8% by helium dilution and +/- 8.3% by body plethysmography; increase in heart rate and minute ventilation for an increase in oxygen uptake of 0.5 1 min-1, +/- 19.4% and +/- 16.3%; distance covered in a 12-minute walking test, +/- 8.2%. The 12-minute distance increased significantly over the first three studies. The considerable variations in the result of these tests in subjects with apparently stable clinical states and without changes in treatment should be considered when assessing the results of changes in treatment."}
{"id": "PMID:483196", "title": "Bronchodilator effects of antiasthmatic cigarette smoke (Datura stramonium).", "content": "In 12 asthmatic patients with mild airway obstruction we have measured the effect on specific airway resistance (sRaw) of inhaling the smoke of one Datura stramonium cigarette. In 11 patients sRaw decreased substantially after the cigarette, the mean maximal decrease being 40% at the 30th minute. In seven patients the subsequent inhalation of 200 micrograms salbutamol caused no further decrease in sRaw. In the remaining four patients salbutamol induced a larger decrease in sRaw than the cigarette smoke. The inhalation, however, of a synthetic anticholinergic agent (SCH 1000, 600 micrograms) proved as effective as salbutamol in these patients. In one patient the cigarette smoke and SCH 1000 produced only a negligible amount of bronchodilatation whereas the bronchial obstruction was reversible with salbutamol. Minor side effects were observed in six patients after the cigarette.", "contents": "Bronchodilator effects of antiasthmatic cigarette smoke (Datura stramonium). In 12 asthmatic patients with mild airway obstruction we have measured the effect on specific airway resistance (sRaw) of inhaling the smoke of one Datura stramonium cigarette. In 11 patients sRaw decreased substantially after the cigarette, the mean maximal decrease being 40% at the 30th minute. In seven patients the subsequent inhalation of 200 micrograms salbutamol caused no further decrease in sRaw. In the remaining four patients salbutamol induced a larger decrease in sRaw than the cigarette smoke. The inhalation, however, of a synthetic anticholinergic agent (SCH 1000, 600 micrograms) proved as effective as salbutamol in these patients. In one patient the cigarette smoke and SCH 1000 produced only a negligible amount of bronchodilatation whereas the bronchial obstruction was reversible with salbutamol. Minor side effects were observed in six patients after the cigarette."}
{"id": "PMID:483197", "title": "Hydropneumopericardium and oesophagitis: a non-fatal case.", "content": "Hydropneumopericardium is a very rare and usually fatal complication of peptic oesophageal ulceration. The patient reported here survived and the report resembles one previously made about a child. In both patients failure to show the fistula radiologically or on endoscopy suggests that rapid spontaneous healing had occurred, and that this was responsible for survival.", "contents": "Hydropneumopericardium and oesophagitis: a non-fatal case. Hydropneumopericardium is a very rare and usually fatal complication of peptic oesophageal ulceration. The patient reported here survived and the report resembles one previously made about a child. In both patients failure to show the fistula radiologically or on endoscopy suggests that rapid spontaneous healing had occurred, and that this was responsible for survival."}
{"id": "PMID:483198", "title": "Effect of hyperlipidaemia on pulmonary diffusing capacity for carbon monoxide.", "content": "There is conflicting evidence on the effect of hyperlipidaemia on pulmonary diffusing capacity for carbon monoxide (DLCO or TLCO) in man. We have measured the carbon monoxide transfer factor per unit alveolar volume (TLCO/VA or KCO) by the single breath method in 25 patients with hyperlipidaemia, and in three normal subjects before and after infusions of an intravenous fat emulsion, Intralipid. Non-smokers with hyperlipidaemia had normal levels of TLCO/VA, whereas some of the smokers showed a slight reduction. In neither group was there any correlation of TLCO/VA with serum triglyceride or cholesterol concentrations. A reduction in triglyceride concentrations of up to five-fold produced by plasma exchange (three studies in two patients) or by dietary manipulation (one patient) had no significant effect on the levels of TLCO/VA. Intralipid infusion in three normal subjects caused a four- to five-fold increase in serum triglyceride concentration but had no effect on TLCO/VA. We conclude that moderate degrees of hyperlipidaemia have no effect on pulmonary diffusion.", "contents": "Effect of hyperlipidaemia on pulmonary diffusing capacity for carbon monoxide. There is conflicting evidence on the effect of hyperlipidaemia on pulmonary diffusing capacity for carbon monoxide (DLCO or TLCO) in man. We have measured the carbon monoxide transfer factor per unit alveolar volume (TLCO/VA or KCO) by the single breath method in 25 patients with hyperlipidaemia, and in three normal subjects before and after infusions of an intravenous fat emulsion, Intralipid. Non-smokers with hyperlipidaemia had normal levels of TLCO/VA, whereas some of the smokers showed a slight reduction. In neither group was there any correlation of TLCO/VA with serum triglyceride or cholesterol concentrations. A reduction in triglyceride concentrations of up to five-fold produced by plasma exchange (three studies in two patients) or by dietary manipulation (one patient) had no significant effect on the levels of TLCO/VA. Intralipid infusion in three normal subjects caused a four- to five-fold increase in serum triglyceride concentration but had no effect on TLCO/VA. We conclude that moderate degrees of hyperlipidaemia have no effect on pulmonary diffusion."}
{"id": "PMID:483202", "title": "A double-blind trial of bromocriptine in steroid dependent asthma.", "content": "The pilot study of Newman Taylor et al (1976) in which, by adding bromocriptine to the full medical treatment, they improved the symptoms of three out of four asthmatic patients, prompted us to carry out this study. Bromocriptine is a dopaminergic agonist that is used in acromegalic patients, as well as an inhibitor of prolactin in doses of 5 to 20 mg a day. We decided to use 15 mg a day, which is usually without major side effects.", "contents": "A double-blind trial of bromocriptine in steroid dependent asthma. The pilot study of Newman Taylor et al (1976) in which, by adding bromocriptine to the full medical treatment, they improved the symptoms of three out of four asthmatic patients, prompted us to carry out this study. Bromocriptine is a dopaminergic agonist that is used in acromegalic patients, as well as an inhibitor of prolactin in doses of 5 to 20 mg a day. We decided to use 15 mg a day, which is usually without major side effects."}
{"id": "PMID:483205", "title": "Peak flow rate records in the diagnosis of occupational asthma due to colophony.", "content": "Peak expiratory flow rate (PEFR) has been measured hourly from waking to sleeping in 29 workers with respiratory symptoms exposed to the fumes of soft soldering fluxes containing colophony (pine resin). Thirty-nine records of mean length 33 days have been analysed, and the results compared with the occupational history and bronchial provocation testing in the same workers. From plots of daily mean, maximum, and minimum PEFR, recurring physiological patterns of asthma emerge. The most common pattern is for asthma to increase with each successive working day. Some workers have an equivalent deterioration each working day. Regular recovery patterns taking one, two, and three days are described. The combination of a three-day recovery pattern and a late asthmatic reaction on Monday results in Monday being the best day of each week. Assessment of these records has shown them to be specific and sensitive, provided the worker was not taking corticosteroids or sodium cromoglycate during the period of the record and that bronchodilator usage was kept constant on days at home and at work. The results of the PEFR records correlate well with bronchial provocation testing, and provide a suitable alternative to this for the diagnosis of mild to moderate occupational asthma. The records are of particular use for screening symptomatic workers whose symptoms appear unlikely to be related to work.", "contents": "Peak flow rate records in the diagnosis of occupational asthma due to colophony. Peak expiratory flow rate (PEFR) has been measured hourly from waking to sleeping in 29 workers with respiratory symptoms exposed to the fumes of soft soldering fluxes containing colophony (pine resin). Thirty-nine records of mean length 33 days have been analysed, and the results compared with the occupational history and bronchial provocation testing in the same workers. From plots of daily mean, maximum, and minimum PEFR, recurring physiological patterns of asthma emerge. The most common pattern is for asthma to increase with each successive working day. Some workers have an equivalent deterioration each working day. Regular recovery patterns taking one, two, and three days are described. The combination of a three-day recovery pattern and a late asthmatic reaction on Monday results in Monday being the best day of each week. Assessment of these records has shown them to be specific and sensitive, provided the worker was not taking corticosteroids or sodium cromoglycate during the period of the record and that bronchodilator usage was kept constant on days at home and at work. The results of the PEFR records correlate well with bronchial provocation testing, and provide a suitable alternative to this for the diagnosis of mild to moderate occupational asthma. The records are of particular use for screening symptomatic workers whose symptoms appear unlikely to be related to work."}
{"id": "PMID:483206", "title": "Factors influencing the prevalence of asthma among first degree relatives of extrinsic and intrinsic asthmatics.", "content": "The prevalence of asthma, hay fever, and eczema was examined in first degree relatives of extrinsic (atopic) and intrinsic (non-atopic) asthmatics attending the asthma clinics of the Brompton Hospital and the Doncaster Royal Infirmary. In both the Doncaster and Brompton populations the prevalence of asthma, hay fever, and eczema was significantly higher among relatives of extrinsic than among relatives of intrinsic asthmatics. Furthermore, the prevalence of these traits tended to be higher among siblings of extrinsic probands with one or both parents affected than among siblings of probands with neither parent affected. Most importantly, the prevalence of asthma among first degree relatives was positively correlated with the prevalence of hay fever or eczema or both among relatives and with the degree of atopy in the probands. These findings are consistent with the results of previous investigations in which the expression of asthma was shown to depend on a genetic predisposition to the trait as well as exposure to environmental provoking agents. We further suggest that the presence of atopy in genetically predisposed individuals increases the risk of developing asthma.", "contents": "Factors influencing the prevalence of asthma among first degree relatives of extrinsic and intrinsic asthmatics. The prevalence of asthma, hay fever, and eczema was examined in first degree relatives of extrinsic (atopic) and intrinsic (non-atopic) asthmatics attending the asthma clinics of the Brompton Hospital and the Doncaster Royal Infirmary. In both the Doncaster and Brompton populations the prevalence of asthma, hay fever, and eczema was significantly higher among relatives of extrinsic than among relatives of intrinsic asthmatics. Furthermore, the prevalence of these traits tended to be higher among siblings of extrinsic probands with one or both parents affected than among siblings of probands with neither parent affected. Most importantly, the prevalence of asthma among first degree relatives was positively correlated with the prevalence of hay fever or eczema or both among relatives and with the degree of atopy in the probands. These findings are consistent with the results of previous investigations in which the expression of asthma was shown to depend on a genetic predisposition to the trait as well as exposure to environmental provoking agents. We further suggest that the presence of atopy in genetically predisposed individuals increases the risk of developing asthma."}
{"id": "PMID:483207", "title": "Postnatal lung growth after repair of diaphragmatic hernia.", "content": "The lungs of two patients who died eight months and 64 months after repair of a left-sided diaphragmatic hernia on the first day of life were examined. Lung volumes were appropriate for the size of the children, and the ratio of right lung volume to left lung volume was also normal in both patients. The lungs, however, were grossly abnormal with evidence of enlargement and destruction of respiratory tissue. The left lung was affected more than the right in both subjects. In one patient the total number of alveoli in the lungs was similar to that of normal children of the same age, but this was because the right lung had more than twice as many alveoli as the left lung. It appears that alveolar multiplication is impaired after repair of diaphragmatic hernia. The number of bronchioles was reduced in the infant with very few alveoli, and there may have been too few bronchioles in the other patient.", "contents": "Postnatal lung growth after repair of diaphragmatic hernia. The lungs of two patients who died eight months and 64 months after repair of a left-sided diaphragmatic hernia on the first day of life were examined. Lung volumes were appropriate for the size of the children, and the ratio of right lung volume to left lung volume was also normal in both patients. The lungs, however, were grossly abnormal with evidence of enlargement and destruction of respiratory tissue. The left lung was affected more than the right in both subjects. In one patient the total number of alveoli in the lungs was similar to that of normal children of the same age, but this was because the right lung had more than twice as many alveoli as the left lung. It appears that alveolar multiplication is impaired after repair of diaphragmatic hernia. The number of bronchioles was reduced in the infant with very few alveoli, and there may have been too few bronchioles in the other patient."}
{"id": "PMID:483208", "title": "Relationship of bacterial and viral infections to exacerbations of asthma.", "content": "Fifty-one asthmatic patients were followed for up to 18 months. During this time 111 exacerbations of wheeze were recorded. Involvement by pathogenic respiratory bacteria and viruses was looked for directly by culture and indirectly by antibody studies. Proof of infection was found in only 12 (10.8%) of the 111 exacerbations. Only eight patients provided sputum samples. Potential bacterial pathogens were found in four. Viruses were isolated in four of 27 exacerbation specimens; significant rises in specific viral antibody titres occurred in three. Six patients developed precipitating antibody to respiratory bacteria over the study but only one in relation to an exacerbation. The study therefore indicated that the great majority of exacerbations of asthma in these patients were not due to respiratory tract infection.", "contents": "Relationship of bacterial and viral infections to exacerbations of asthma. Fifty-one asthmatic patients were followed for up to 18 months. During this time 111 exacerbations of wheeze were recorded. Involvement by pathogenic respiratory bacteria and viruses was looked for directly by culture and indirectly by antibody studies. Proof of infection was found in only 12 (10.8%) of the 111 exacerbations. Only eight patients provided sputum samples. Potential bacterial pathogens were found in four. Viruses were isolated in four of 27 exacerbation specimens; significant rises in specific viral antibody titres occurred in three. Six patients developed precipitating antibody to respiratory bacteria over the study but only one in relation to an exacerbation. The study therefore indicated that the great majority of exacerbations of asthma in these patients were not due to respiratory tract infection."}
{"id": "PMID:483209", "title": "Ventilatory function in the Eisenmenger syndrome.", "content": "Ventilatory function and carbon monoxide transfer factor were studied in nine adult patients with post-tricuspid intracardiac defects and the Eisenmenger syndrome. A consistent mild defect of ventilatory function was found, with raised residual volume and closing capacity and reduction of other lung volumes and maximal expiratory flows. Maximal expiratory flow was particularly reduced at low lung volumes. One of the four subjects in whom pressure-volume studies were performed showed loss of normal elastic recoil at low lung volumes. A significant poorly ventilated space was excluded since the volume of distribution of helium during a 10 second breath-hold at full inspiration was close to plethysmographically measured total lung capacity. Carbon monoxide transfer factor, after correction for haemoglobin concentration, was 77% of predicted normal values. Sequential assessment of carbon monoxide transfer factor might be useful in the early diagnosis of pulmonary vascular disease in infants with large intracardiac defects, since it is likely to be raised early due to high pulmonary blood flow and will fall with the development of pulmonary vascular disease.", "contents": "Ventilatory function in the Eisenmenger syndrome. Ventilatory function and carbon monoxide transfer factor were studied in nine adult patients with post-tricuspid intracardiac defects and the Eisenmenger syndrome. A consistent mild defect of ventilatory function was found, with raised residual volume and closing capacity and reduction of other lung volumes and maximal expiratory flows. Maximal expiratory flow was particularly reduced at low lung volumes. One of the four subjects in whom pressure-volume studies were performed showed loss of normal elastic recoil at low lung volumes. A significant poorly ventilated space was excluded since the volume of distribution of helium during a 10 second breath-hold at full inspiration was close to plethysmographically measured total lung capacity. Carbon monoxide transfer factor, after correction for haemoglobin concentration, was 77% of predicted normal values. Sequential assessment of carbon monoxide transfer factor might be useful in the early diagnosis of pulmonary vascular disease in infants with large intracardiac defects, since it is likely to be raised early due to high pulmonary blood flow and will fall with the development of pulmonary vascular disease."}
{"id": "PMID:483210", "title": "Relationship between bronchial and arterial diameters in normal human lungs.", "content": "In order to find an objective method for measuring narrowing of small airways, eight lungs from four people without lung disease were inflated and fixed at 25 cm of water pressure. Eight to nine blocks were taken at random from each of six parasaggital slices from each lung, sectioned, and stained. The size of the bronchioles (airways without cartilage) in relation to their accompanying arteries was determined. The internal bronchiolar diameter was compared with three different arterial diameters (the internal, external medial, and external adventitial). The ratio of the internal bronchiolar diameter to the external adventitial arterial diameter was constant between lungs (0.62 +/- 0.02) and independent of the method of inflation or the position within the lungs. This ratio may provide a useful index of bronchiolar narrowing in disease.", "contents": "Relationship between bronchial and arterial diameters in normal human lungs. In order to find an objective method for measuring narrowing of small airways, eight lungs from four people without lung disease were inflated and fixed at 25 cm of water pressure. Eight to nine blocks were taken at random from each of six parasaggital slices from each lung, sectioned, and stained. The size of the bronchioles (airways without cartilage) in relation to their accompanying arteries was determined. The internal bronchiolar diameter was compared with three different arterial diameters (the internal, external medial, and external adventitial). The ratio of the internal bronchiolar diameter to the external adventitial arterial diameter was constant between lungs (0.62 +/- 0.02) and independent of the method of inflation or the position within the lungs. This ratio may provide a useful index of bronchiolar narrowing in disease."}
{"id": "PMID:483211", "title": "Lung function in healthy British women.", "content": "The forces expiratory volume, total lung capacity, transfer factor (diffusing capacity), and their subdivisions have been measured in 113 healthy British women aged 27 to 74 years of whom 47 were current smokers and 66 were lifetime non-smokers. The results have been analysed in terms of age, stature, mass, body fat, and smoking. In addition to their relation to stature and to age, the inspiratory capacity was positively correlated with indices of body muscle while the residual volume, expiratory reserve volume, and total lung capacity were inversely correlated with the percentage of body mass that is fat or with mass divided by the square of stature. The inverse correlation between total lung capacity and age was apparently due to the quantity of body fat increasing with age. The transfer factor and its subdivisions were inversely correlated with smoking. In this study the forced expiratory volume and vital capacity were independent of both fat and smoking; the transfer factor was independent of the physiological response to exercise. The results provide reference values for lung function in British women.", "contents": "Lung function in healthy British women. The forces expiratory volume, total lung capacity, transfer factor (diffusing capacity), and their subdivisions have been measured in 113 healthy British women aged 27 to 74 years of whom 47 were current smokers and 66 were lifetime non-smokers. The results have been analysed in terms of age, stature, mass, body fat, and smoking. In addition to their relation to stature and to age, the inspiratory capacity was positively correlated with indices of body muscle while the residual volume, expiratory reserve volume, and total lung capacity were inversely correlated with the percentage of body mass that is fat or with mass divided by the square of stature. The inverse correlation between total lung capacity and age was apparently due to the quantity of body fat increasing with age. The transfer factor and its subdivisions were inversely correlated with smoking. In this study the forced expiratory volume and vital capacity were independent of both fat and smoking; the transfer factor was independent of the physiological response to exercise. The results provide reference values for lung function in British women."}
{"id": "PMID:483213", "title": "Two malignant pleural mesotheliomas with unusual histological features.", "content": "Morphologically, mesotheliomas may be composed of epithelial and/or sarcomatous elements with various patterns, such as tubular, papillary, tubulopapillary, and diffuse epithelial or mixtures of these. Two cases are descirbed in which, in addition to typical mesothelioma, there was cartilage with foci of calcification and ossificated with the mesothelioma, which suggested that they formed an integral part of the tumour. One of the cases also showed a cuff of cartilage and bone round blood vessels and bronchioles in the lung parenchyma. The patholgenesis could be explained if the mesothelial cell is considered to be totipotent and able to give rise to epithelial and connective tissue elements. Other theories that must be considered are: that there are two separate neoplasms; that there is a circulating substance, perhaps induced by the mesothelioma, which stimulated the cartilage and bone formation; and that the cartilage and bone were due to a previous or associated infection such as tuberculosis. Calcification is also common in asbestotic pleural plaques.", "contents": "Two malignant pleural mesotheliomas with unusual histological features. Morphologically, mesotheliomas may be composed of epithelial and/or sarcomatous elements with various patterns, such as tubular, papillary, tubulopapillary, and diffuse epithelial or mixtures of these. Two cases are descirbed in which, in addition to typical mesothelioma, there was cartilage with foci of calcification and ossificated with the mesothelioma, which suggested that they formed an integral part of the tumour. One of the cases also showed a cuff of cartilage and bone round blood vessels and bronchioles in the lung parenchyma. The patholgenesis could be explained if the mesothelial cell is considered to be totipotent and able to give rise to epithelial and connective tissue elements. Other theories that must be considered are: that there are two separate neoplasms; that there is a circulating substance, perhaps induced by the mesothelioma, which stimulated the cartilage and bone formation; and that the cartilage and bone were due to a previous or associated infection such as tuberculosis. Calcification is also common in asbestotic pleural plaques."}
{"id": "PMID:483214", "title": "Recurrent pulmonary thromboembolism presenting with cardiac arrhythmias.", "content": "Pulmonary emboli seldom recur, and when recurrence does occur it is not associated with permanent sequelae unless there is progressive pulmonary arterial hypertension. Five patients with clinical and perfusion lung scan evidence of recurrent pulmonary embolism presented with abnormal cardiac rhythms without evidence of progressive pulmonary hypertension. Twenty-four-hour ambulatory electrocardiographic monitoring was valuable in diagnosis and in assessing the effectiveness of treatment. Although palpitation was the main complaint, other symptoms included tiredness, mild exertional dyspnoea, and chest discomfort unrelated to effort. Symptomatic improvement coincided with objective evidence of improvement from repeat lung scans and 24-hour ECG records. Antiarrhythmic agents controlled the arrhythmias but were subsequently withdrawn without the return of symptoms. Four of the five patients continued to take anticoagulants for two years. We believe that these five patients represent a group of patients with recurrent pulmonary emboli and a recognisable clinical picture dominated by arrhythmias unrelated to progressive pulmonary arterial hypertension. Long-term anticoagulant treatment was associated with clinical improvement.", "contents": "Recurrent pulmonary thromboembolism presenting with cardiac arrhythmias. Pulmonary emboli seldom recur, and when recurrence does occur it is not associated with permanent sequelae unless there is progressive pulmonary arterial hypertension. Five patients with clinical and perfusion lung scan evidence of recurrent pulmonary embolism presented with abnormal cardiac rhythms without evidence of progressive pulmonary hypertension. Twenty-four-hour ambulatory electrocardiographic monitoring was valuable in diagnosis and in assessing the effectiveness of treatment. Although palpitation was the main complaint, other symptoms included tiredness, mild exertional dyspnoea, and chest discomfort unrelated to effort. Symptomatic improvement coincided with objective evidence of improvement from repeat lung scans and 24-hour ECG records. Antiarrhythmic agents controlled the arrhythmias but were subsequently withdrawn without the return of symptoms. Four of the five patients continued to take anticoagulants for two years. We believe that these five patients represent a group of patients with recurrent pulmonary emboli and a recognisable clinical picture dominated by arrhythmias unrelated to progressive pulmonary arterial hypertension. Long-term anticoagulant treatment was associated with clinical improvement."}
{"id": "PMID:483215", "title": "Pulmonary metastatic calcification with respiratory insufficiency in patients on maintenance haemodialysis.", "content": "A uraemic patient undergoing chronic haemodialysis developed diffuse metastatic pulmonary calcification and died from acute respiratory insufficiency after renal transplantation. Thirteen similar cases previously published are reviewed, with emphasis on the clinical and anatomical features of such calcinosis. The pathogenesis of this calcification in patients on maintenance haemodialysis and some rules for its prevention are discussed.", "contents": "Pulmonary metastatic calcification with respiratory insufficiency in patients on maintenance haemodialysis. A uraemic patient undergoing chronic haemodialysis developed diffuse metastatic pulmonary calcification and died from acute respiratory insufficiency after renal transplantation. Thirteen similar cases previously published are reviewed, with emphasis on the clinical and anatomical features of such calcinosis. The pathogenesis of this calcification in patients on maintenance haemodialysis and some rules for its prevention are discussed."}
{"id": "PMID:483216", "title": "Pulmonary gas exchange during exercise in young asthmatic patients.", "content": "Pulmonary function was examined in 19 young asthmatic patients at rest and during two levels of excercise. Findings at rest included decreased flow rates, increased residual volume, normal minute (VE) and alveolar (VA) ventilation, increased ratio of physiological dead space to tidal volume (VD/VT), increased alveolar-arterial oxygen tension difference (A-a PO2), and mild arterial hypoxaemia and desaturation. On exercise there was a normal increase in VE and VA, the VD/VT and the A-a PO2 decreased towards normal, and arterial oxygen tension improved, approaching normal levels. Significant acidosis did not develop.", "contents": "Pulmonary gas exchange during exercise in young asthmatic patients. Pulmonary function was examined in 19 young asthmatic patients at rest and during two levels of excercise. Findings at rest included decreased flow rates, increased residual volume, normal minute (VE) and alveolar (VA) ventilation, increased ratio of physiological dead space to tidal volume (VD/VT), increased alveolar-arterial oxygen tension difference (A-a PO2), and mild arterial hypoxaemia and desaturation. On exercise there was a normal increase in VE and VA, the VD/VT and the A-a PO2 decreased towards normal, and arterial oxygen tension improved, approaching normal levels. Significant acidosis did not develop."}
{"id": "PMID:483220", "title": "An unusual complication of colonic interposition for oesophageal replacement.", "content": "We report a case of delayed fibrosis of the interposed left colon in a 65-year-old man after an operation for peptic oesophageal stricture and suggest that short-term postoperative ventilation should be used in patients undergoing oesophageal replacement by means of colonic interposition.", "contents": "An unusual complication of colonic interposition for oesophageal replacement. We report a case of delayed fibrosis of the interposed left colon in a 65-year-old man after an operation for peptic oesophageal stricture and suggest that short-term postoperative ventilation should be used in patients undergoing oesophageal replacement by means of colonic interposition."}
{"id": "PMID:483222", "title": "Superior vena caval stenosis: a complication of transvenous endocardial pacing.", "content": "Superior vena caval obstruction is a rare complication of transvenous endocardial pacing and is usually the result of thrombus formation round the pacing electrode (Kosowsky and Barr, 1972). We report a case of superior vena caval obstruction without thrombus formation secondary to localised stenosis at the site of the proximal cut end of a retracted endocardial electrode. This complication of transvenous pacing electrodes has not been described previously.", "contents": "Superior vena caval stenosis: a complication of transvenous endocardial pacing. Superior vena caval obstruction is a rare complication of transvenous endocardial pacing and is usually the result of thrombus formation round the pacing electrode (Kosowsky and Barr, 1972). We report a case of superior vena caval obstruction without thrombus formation secondary to localised stenosis at the site of the proximal cut end of a retracted endocardial electrode. This complication of transvenous pacing electrodes has not been described previously."}
{"id": "PMID:483234", "title": "Occlusive coronary thrombosis and oral anticoagulants.", "content": "The results of post-mortem examination in 173 patients followed over an average period of five and a half years after their initial myocardial infarction are described. These 173 patients were divided into four groups according to whether or not they had received an oral anticoagulant and if so how adequately. An index of coronary and myocardial lesions was established for each heart. Recent occlusive coronary and myocardial lesions was established for each heart. Recent occlusive coronary thromboses were four times less frequent in the group of patients who had received adequate anticoagulant therapy than in the other three groups of patients (p less than 0,001). There was no significant difference between the inadequately treated groups and the untreated group. The recurrences of myocardial infarction were associated in 90 per cent of the cases with a recent occlusive thrombosis in the corresponding coronary artery and were found four times less frequently in the group subjected to effective long-term anticoagulant therapy (p less than 0,001).", "contents": "Occlusive coronary thrombosis and oral anticoagulants. The results of post-mortem examination in 173 patients followed over an average period of five and a half years after their initial myocardial infarction are described. These 173 patients were divided into four groups according to whether or not they had received an oral anticoagulant and if so how adequately. An index of coronary and myocardial lesions was established for each heart. Recent occlusive coronary and myocardial lesions was established for each heart. Recent occlusive coronary thromboses were four times less frequent in the group of patients who had received adequate anticoagulant therapy than in the other three groups of patients (p less than 0,001). There was no significant difference between the inadequately treated groups and the untreated group. The recurrences of myocardial infarction were associated in 90 per cent of the cases with a recent occlusive thrombosis in the corresponding coronary artery and were found four times less frequently in the group subjected to effective long-term anticoagulant therapy (p less than 0,001)."}
{"id": "PMID:483235", "title": "Is the antithrombotic activity of \"antiplatelet\" drugs based on protection of endothelium?", "content": "In only five \"antiplatelet\" agents has the antithrombotic activity been confirmed both clinically and experimentally. These include acetylsalicylic acid, dipyridamole, clofibrate, hydroxychloroquine and sulfinpyrazone. Whereas only acetylsalicylic acid has a demonstrable effect on platelet aggregation in doses usually applied, the effects of all remaining drugs are more easily explained by their influence on the vessel wall. This assumption is supported by the finding that all five drugs possess a stabilizing effect on endothelium in doses corresponding closely to the clinical dose range. The method based on counting detached endothelial cells in blood after a standard stimulus was used to demonstrate this effect.", "contents": "Is the antithrombotic activity of \"antiplatelet\" drugs based on protection of endothelium? In only five \"antiplatelet\" agents has the antithrombotic activity been confirmed both clinically and experimentally. These include acetylsalicylic acid, dipyridamole, clofibrate, hydroxychloroquine and sulfinpyrazone. Whereas only acetylsalicylic acid has a demonstrable effect on platelet aggregation in doses usually applied, the effects of all remaining drugs are more easily explained by their influence on the vessel wall. This assumption is supported by the finding that all five drugs possess a stabilizing effect on endothelium in doses corresponding closely to the clinical dose range. The method based on counting detached endothelial cells in blood after a standard stimulus was used to demonstrate this effect."}
{"id": "PMID:483237", "title": "The shape of the fibrinogen molecule: sausage or banana?", "content": "The experimental results and the interpretation of small angle X-ray scattering measurements by the author and Hammel (1975) and of small angle neutron scattering measurements by Marguerie and Stuhrmann (1976) on dilute solutions of bovine fibrinogen are compared. The potential of both methods to discern straight and bent cylindrical structures is demonstrated. The study arrives at the conclusion that the question whether the fibrinogen molecule resembles more a banana or a straight sausage cannot be safely decided by the available data. This justifies the previous suggestion of the author to imagine the fibrinogen molecule as a swollen sausage 450 A long and 90 A thick with conceivable flexibility, a molecular model of sufficient universality to include the banana-shaped model of Marguerie and Stuhrmann (1976) as a special case.", "contents": "The shape of the fibrinogen molecule: sausage or banana? The experimental results and the interpretation of small angle X-ray scattering measurements by the author and Hammel (1975) and of small angle neutron scattering measurements by Marguerie and Stuhrmann (1976) on dilute solutions of bovine fibrinogen are compared. The potential of both methods to discern straight and bent cylindrical structures is demonstrated. The study arrives at the conclusion that the question whether the fibrinogen molecule resembles more a banana or a straight sausage cannot be safely decided by the available data. This justifies the previous suggestion of the author to imagine the fibrinogen molecule as a swollen sausage 450 A long and 90 A thick with conceivable flexibility, a molecular model of sufficient universality to include the banana-shaped model of Marguerie and Stuhrmann (1976) as a special case."}
{"id": "PMID:483238", "title": "Fibrinogen: agreement of experimental and calculated hydrodynamic data with electron-microscopic models.", "content": "There is no general agreement on the size and shape of the fibrinogen molecule. We have studied the diffusion of the fibrinogen in solution by means of dynamic light scattering, nanosecond fluorescence depolarization and analytical ultracentrifugation. The results obtained under physiological concentration, pH an ionic strength are DT = 2.0 X 10(7) cm2sec-1, DR perpendicular = 40'000 sec 1. Nanosecond fluorescence depolarization yielded DR parallel = 1.6 x 10(6) sec-1. Tentatively this value is interpreted as DR parallel, namely rotational diffusion about the major axis of the molecule. The sedimentation coefficient is 8.1 S. The hydrodynamic parameters derived from our measurements were compared with those calculated on the basis of the models proposed by Hall and Slayter, Hudry-Clergeon and Marguerie et al., Bachmann and Lederer, and K\u00f6ppel. The agreement is poor even if the degree of hydration is varied within wide limits. However, satisfactory agreement can be achieved by assuming a flexible molecule of about 900 A length corresponding to two end-to-end bound trinodular structures of the Hall and Slayter type, with a nodule diameter of about 45 A. Experimental evidence indicates that the discrepancies between the different models might be due to different techniques of sample preparation leading to different conformations of the molecule.", "contents": "Fibrinogen: agreement of experimental and calculated hydrodynamic data with electron-microscopic models. There is no general agreement on the size and shape of the fibrinogen molecule. We have studied the diffusion of the fibrinogen in solution by means of dynamic light scattering, nanosecond fluorescence depolarization and analytical ultracentrifugation. The results obtained under physiological concentration, pH an ionic strength are DT = 2.0 X 10(7) cm2sec-1, DR perpendicular = 40'000 sec 1. Nanosecond fluorescence depolarization yielded DR parallel = 1.6 x 10(6) sec-1. Tentatively this value is interpreted as DR parallel, namely rotational diffusion about the major axis of the molecule. The sedimentation coefficient is 8.1 S. The hydrodynamic parameters derived from our measurements were compared with those calculated on the basis of the models proposed by Hall and Slayter, Hudry-Clergeon and Marguerie et al., Bachmann and Lederer, and K\u00f6ppel. The agreement is poor even if the degree of hydration is varied within wide limits. However, satisfactory agreement can be achieved by assuming a flexible molecule of about 900 A length corresponding to two end-to-end bound trinodular structures of the Hall and Slayter type, with a nodule diameter of about 45 A. Experimental evidence indicates that the discrepancies between the different models might be due to different techniques of sample preparation leading to different conformations of the molecule."}
{"id": "PMID:483241", "title": "Separation of fibrinogen degradation products (FDP) by means of adsorption to insolubilized fibrinmonomer (FM-ag).", "content": "The phenomenon of complex formation between fibrinmonomer and fibrinogen degradation products was investigated by means of adsorption of FDP to insolubilized thrombin-modified fibrinogen (FM-ag). Since it could be demonstrated that there are different adsorption characteristics for early FDP and late FDP, the possibility of separation of FDP by means of affinity chromatography on FM-ag columns was evaluated using plasmic digests of 3H-Ac-labelled fibrinogen. The identification of FDP was performed by disc-electrophoresis. The results indicate that the adsorption of early FDP is comparable to the behaviour of fibrinogen, whereas late FDP show essential difference in the affinity towards FM-ag, evident by the result that fragment E adsorbs only to a minimal extents. Fragments D and E derived from fibrinogen as well as from non-crosslinked fibrin, revealed identical adsorption characteristics. Under specified conditions the procedure is suitable as a preparative method for the separation of fragments D and E.", "contents": "Separation of fibrinogen degradation products (FDP) by means of adsorption to insolubilized fibrinmonomer (FM-ag). The phenomenon of complex formation between fibrinmonomer and fibrinogen degradation products was investigated by means of adsorption of FDP to insolubilized thrombin-modified fibrinogen (FM-ag). Since it could be demonstrated that there are different adsorption characteristics for early FDP and late FDP, the possibility of separation of FDP by means of affinity chromatography on FM-ag columns was evaluated using plasmic digests of 3H-Ac-labelled fibrinogen. The identification of FDP was performed by disc-electrophoresis. The results indicate that the adsorption of early FDP is comparable to the behaviour of fibrinogen, whereas late FDP show essential difference in the affinity towards FM-ag, evident by the result that fragment E adsorbs only to a minimal extents. Fragments D and E derived from fibrinogen as well as from non-crosslinked fibrin, revealed identical adsorption characteristics. Under specified conditions the procedure is suitable as a preparative method for the separation of fragments D and E."}
{"id": "PMID:483242", "title": "Adsorption of fibrinogen and fragment D of fibrinogen onto the insolubilized alpha-chain of fibrin.", "content": "After thrombin treatment insolubilized fibrinmonomer, which is obtained from insolubilized fibrinogen covalently bound to agarose, adsorbs soluble fibrin and its derivatives from solutions. The immobilized proteins are attached to the agarose by the 'A' alpha-chain. After reduction of the disulfide bridges the beta- and gamma-chains can be removed from the agarose. After thrombin treatment the immobilized alpha-chain adsorbs fibrinogen and fragment D. To some extent the beta- and gamma-chain do not seem necessary for the adsorption. The amount adsorbed increases, when thrombin treatment of the insolubilized protein follows the reduction process. This may indicate that the fibrinopeptides 'A' of the insolubilized alpha-chain are better accessible after the removal of the beta- and gamma-chains.", "contents": "Adsorption of fibrinogen and fragment D of fibrinogen onto the insolubilized alpha-chain of fibrin. After thrombin treatment insolubilized fibrinmonomer, which is obtained from insolubilized fibrinogen covalently bound to agarose, adsorbs soluble fibrin and its derivatives from solutions. The immobilized proteins are attached to the agarose by the 'A' alpha-chain. After reduction of the disulfide bridges the beta- and gamma-chains can be removed from the agarose. After thrombin treatment the immobilized alpha-chain adsorbs fibrinogen and fragment D. To some extent the beta- and gamma-chain do not seem necessary for the adsorption. The amount adsorbed increases, when thrombin treatment of the insolubilized protein follows the reduction process. This may indicate that the fibrinopeptides 'A' of the insolubilized alpha-chain are better accessible after the removal of the beta- and gamma-chains."}
{"id": "PMID:483243", "title": "A thiocholine ester of cinnamic acid inhibits crosslinking of fibrin without specific binding to donor lysines.", "content": "In the presence of activated factor XIII, 2-diethylbenzyl-aminoethylthiol-14C-transcinnamate bromide completely inhibited the crosslinking of fibrin. However, all three fibrin chains bound the cinnamic acid, and, in the alpha- and gamma-chains, the binding of label was not restricted to the crosslinking donor sites as might be expected. Furthermore, even in the absence of activated factor XIII, fibrinogen and fibrin incorporated cinnamic acid. Thus, as well as reacting with the functional thiol group of factor XIII, the thiocholine ester of cinnamic acid is incorporated non-specifically throughout the fibrinogen and fibrin subunit chains. The thiocholine ester used differs in this respect from dansyl cadaverine which is incorporated enzymatically and exclusively to the (acceptor) sites involved in crosslinking. Thiocholine ester of cinnamic acid cannot be used as a label for localization of specific crosslinking donor sites.", "contents": "A thiocholine ester of cinnamic acid inhibits crosslinking of fibrin without specific binding to donor lysines. In the presence of activated factor XIII, 2-diethylbenzyl-aminoethylthiol-14C-transcinnamate bromide completely inhibited the crosslinking of fibrin. However, all three fibrin chains bound the cinnamic acid, and, in the alpha- and gamma-chains, the binding of label was not restricted to the crosslinking donor sites as might be expected. Furthermore, even in the absence of activated factor XIII, fibrinogen and fibrin incorporated cinnamic acid. Thus, as well as reacting with the functional thiol group of factor XIII, the thiocholine ester of cinnamic acid is incorporated non-specifically throughout the fibrinogen and fibrin subunit chains. The thiocholine ester used differs in this respect from dansyl cadaverine which is incorporated enzymatically and exclusively to the (acceptor) sites involved in crosslinking. Thiocholine ester of cinnamic acid cannot be used as a label for localization of specific crosslinking donor sites."}
{"id": "PMID:483244", "title": "Fibrinogen Zurich I: impaired release of fibrinopeptide A.", "content": "Fibrinogen Zurich I is characterized by an abnormal fibrin monomer polymerization. It consists of two fractions of molecules, one with a normal aggregation and one not aggregating at all and interfering with the aggregation of the normal population. Using a radioimmunoassay for fibrinopeptide A, only approximately half of the expected fibrinopeptide A could be recovered after thrombin or Defibrase proteolysis. The defective fibrinopeptide A release could be confirmed by measurement of the N-terminal Gly/Tyr ratio. It is likely that the abnormal fibrin monomer aggregation of the abnormal fraction of fibrinogen Zurich I is due to the defective fibrinopeptide A release of this fraction.", "contents": "Fibrinogen Zurich I: impaired release of fibrinopeptide A. Fibrinogen Zurich I is characterized by an abnormal fibrin monomer polymerization. It consists of two fractions of molecules, one with a normal aggregation and one not aggregating at all and interfering with the aggregation of the normal population. Using a radioimmunoassay for fibrinopeptide A, only approximately half of the expected fibrinopeptide A could be recovered after thrombin or Defibrase proteolysis. The defective fibrinopeptide A release could be confirmed by measurement of the N-terminal Gly/Tyr ratio. It is likely that the abnormal fibrin monomer aggregation of the abnormal fraction of fibrinogen Zurich I is due to the defective fibrinopeptide A release of this fraction."}
{"id": "PMID:483246", "title": "Separation of plasminogen activators from human plasma and a comparison with activators from human uterine tissue and urine.", "content": "Normal human plasma contains acid-stable as well as labile plasminogen activators. The activity of activators in plasma euglobulins was inhibited by EACA in an uniform pattern, similar to that obtained with the major activators in human uterine tissue or with the purified porcine tissue activator, but different from the patterns obtained with plasmin or with urokinase. Gel filtration at high ionic strength separated activators corresponding to particle sizes of 60,000 dalton and about 10,000 dalton, corresponding to two activators similarly obtained from human tissue. The 60,000 dalton activator was precipitated in the euglobulin fraction. Its concentration increased in plasma after exercise. The 10,000 dalton activator was found mainly in the supernatant. Gel filtration in 0.15 M solutions yielded activators in fractions of molecular sizes of 100-140,000 dalton and 200,000 dalton or larger. The activity of normal and exercise euglobulins was inhibited by antiserum to a plasminogen activator prepared from porcine tissue, but it was not inhibited by antiserum to urokinase. Plasminogen activators in human plasma euglobulins resembled immunochemically the activators in human uterine tissue.", "contents": "Separation of plasminogen activators from human plasma and a comparison with activators from human uterine tissue and urine. Normal human plasma contains acid-stable as well as labile plasminogen activators. The activity of activators in plasma euglobulins was inhibited by EACA in an uniform pattern, similar to that obtained with the major activators in human uterine tissue or with the purified porcine tissue activator, but different from the patterns obtained with plasmin or with urokinase. Gel filtration at high ionic strength separated activators corresponding to particle sizes of 60,000 dalton and about 10,000 dalton, corresponding to two activators similarly obtained from human tissue. The 60,000 dalton activator was precipitated in the euglobulin fraction. Its concentration increased in plasma after exercise. The 10,000 dalton activator was found mainly in the supernatant. Gel filtration in 0.15 M solutions yielded activators in fractions of molecular sizes of 100-140,000 dalton and 200,000 dalton or larger. The activity of normal and exercise euglobulins was inhibited by antiserum to a plasminogen activator prepared from porcine tissue, but it was not inhibited by antiserum to urokinase. Plasminogen activators in human plasma euglobulins resembled immunochemically the activators in human uterine tissue."}
{"id": "PMID:483247", "title": "Influence of moderate and strenuous daily physical activity on fibrinolytic activity of blood: possibility of plasminogen activator stores depletion.", "content": "Fibrinolysis was studied in 10 alpinists during regular physical activity of different intensity. Blood was sampled at rest and after exposure to submaximal workload on the treadmill on three occasions: before and after 6 months physical conditioning (moderate physical activity), and after 6 weeks of an alpinistic expedition (strenuous physical activity). Measurements included submaximal working capacity, fibrinogen, euglobulin clot lysis time (ELT), whole plasma clot lysis time, and estimations derived from ELT--percent increase in fibrinolytic activity after exercise (RFS), and absolute increase in fibrinolytic activity after exercise (PAR). Regular moderate activity increased the resting level of ELT, but strenuous activity decreased is. After each treadmill testing, a marked increase in fibrinolytic activity was observed. RFS was unaltered at all three testings. PAR increased after moderate activity, but decreased after strenuous activity. The results indicate that regular physical activity can lead from enhanced to decreased resting activity of plasminogen activator in blood. It is presumed that increased release of activator during prolonged stress causes partial depletion of endothelial stores with the consequence of decreased activator activity in the blood.", "contents": "Influence of moderate and strenuous daily physical activity on fibrinolytic activity of blood: possibility of plasminogen activator stores depletion. Fibrinolysis was studied in 10 alpinists during regular physical activity of different intensity. Blood was sampled at rest and after exposure to submaximal workload on the treadmill on three occasions: before and after 6 months physical conditioning (moderate physical activity), and after 6 weeks of an alpinistic expedition (strenuous physical activity). Measurements included submaximal working capacity, fibrinogen, euglobulin clot lysis time (ELT), whole plasma clot lysis time, and estimations derived from ELT--percent increase in fibrinolytic activity after exercise (RFS), and absolute increase in fibrinolytic activity after exercise (PAR). Regular moderate activity increased the resting level of ELT, but strenuous activity decreased is. After each treadmill testing, a marked increase in fibrinolytic activity was observed. RFS was unaltered at all three testings. PAR increased after moderate activity, but decreased after strenuous activity. The results indicate that regular physical activity can lead from enhanced to decreased resting activity of plasminogen activator in blood. It is presumed that increased release of activator during prolonged stress causes partial depletion of endothelial stores with the consequence of decreased activator activity in the blood."}
{"id": "PMID:483248", "title": "Factor XII-dependent fibrinolysis: a double function of plasma kallikrein and the occurrence of a previously undescribed factor XII- and kallikrein-dependent plasminogen proactivator.", "content": "Fibrinolytic studies in euglobulin fractions of Fletcher trait plasma (deficient in prekallikrein) revealed reduced activities as compared to normal plasma. A quantitative assay for total plasminogen activator plus proactivator in plasma showed that the amount in Fletcher trait patients is about half of normal (normal = +/- 100 blood activator units [BAU]/ml). Plasma kallikrein partially purified in a high and low molecular weight form exerted plasminogen activator activity amounting to 10-15 BAU/ml plasma. So, the absence of kallikrein in the deficient plasma cannot fully account for the reduction in activator activity. Additions of kallikrein preparations or normal plasma fractions resulted in additional activator activity in Fletcher trait plasma which was assessed at 30-40 BAU/ml. This activity was assumed to originate from a previously undescribed plasminogen proactivator whose activation is kallikrein- and factor XII-dependent. Fractionation experiments demonstrated the presence of two major activities and a minor activity caused by kallikrein in normal plasma. It is concluded that plasma kallikrein has two functions in the generation of factor XII-dependent fibrinolytic activity: one as a direct plasminogen activator and another as a factor in the activation of a major factor XII-dependent plasminogen proactivator.", "contents": "Factor XII-dependent fibrinolysis: a double function of plasma kallikrein and the occurrence of a previously undescribed factor XII- and kallikrein-dependent plasminogen proactivator. Fibrinolytic studies in euglobulin fractions of Fletcher trait plasma (deficient in prekallikrein) revealed reduced activities as compared to normal plasma. A quantitative assay for total plasminogen activator plus proactivator in plasma showed that the amount in Fletcher trait patients is about half of normal (normal = +/- 100 blood activator units [BAU]/ml). Plasma kallikrein partially purified in a high and low molecular weight form exerted plasminogen activator activity amounting to 10-15 BAU/ml plasma. So, the absence of kallikrein in the deficient plasma cannot fully account for the reduction in activator activity. Additions of kallikrein preparations or normal plasma fractions resulted in additional activator activity in Fletcher trait plasma which was assessed at 30-40 BAU/ml. This activity was assumed to originate from a previously undescribed plasminogen proactivator whose activation is kallikrein- and factor XII-dependent. Fractionation experiments demonstrated the presence of two major activities and a minor activity caused by kallikrein in normal plasma. It is concluded that plasma kallikrein has two functions in the generation of factor XII-dependent fibrinolytic activity: one as a direct plasminogen activator and another as a factor in the activation of a major factor XII-dependent plasminogen proactivator."}
{"id": "PMID:483249", "title": "Ionophoretic activities of phospholipids on human platelets.", "content": "The effects of phospholipids on calcium uptake by human platelets were investigated utilizing 45CaCl2. Among the phospholipids tested, cardiolipin and phosphatidic acid exerted a significant increase of calcium uptake by platelets. Calcium uptake was dependent upon cardiolipin concentration and incubation time. These phospholipids enhanced platelet aggregation induced by ADP (2 microM) or epinephrine (0.5 microgram/ml). Cardiolipin caused the significant release of serotonin from platelets in the absence of externally added calcium in the medium. These results may suggest that phospholipids activate platelets through their ionophoretic activities.", "contents": "Ionophoretic activities of phospholipids on human platelets. The effects of phospholipids on calcium uptake by human platelets were investigated utilizing 45CaCl2. Among the phospholipids tested, cardiolipin and phosphatidic acid exerted a significant increase of calcium uptake by platelets. Calcium uptake was dependent upon cardiolipin concentration and incubation time. These phospholipids enhanced platelet aggregation induced by ADP (2 microM) or epinephrine (0.5 microgram/ml). Cardiolipin caused the significant release of serotonin from platelets in the absence of externally added calcium in the medium. These results may suggest that phospholipids activate platelets through their ionophoretic activities."}
{"id": "PMID:483250", "title": "Abnormalities of platelet adenine nucleotides in patients with myeloproliferative disorders.", "content": "Platelet aggregation and adenine nucleotides in platelets have been studied in thirteen patients with myeloproliferative disorders. ADP induced aggregation was abnormal in two patients, but collagen induced aggregation was impaired in 11 patients. The concentrations of ATP and ADP in resting platelets in the patients with abnormal aggregation were significantly less than those in normal subjects. Marked reduction of the amounts of both nucleotides released into plasma was also observed after stimulation of collagen in these patients. Platelets in the patients with normal functions contained almost normal amounts of adenine nucleotides. We discussed the relationship between platelet dysfunction and adenine nucleotides in platelets of myeloproliferative disorders and concluded that platelet dysfunction was mainly attributable to reduction of releasable ADP.", "contents": "Abnormalities of platelet adenine nucleotides in patients with myeloproliferative disorders. Platelet aggregation and adenine nucleotides in platelets have been studied in thirteen patients with myeloproliferative disorders. ADP induced aggregation was abnormal in two patients, but collagen induced aggregation was impaired in 11 patients. The concentrations of ATP and ADP in resting platelets in the patients with abnormal aggregation were significantly less than those in normal subjects. Marked reduction of the amounts of both nucleotides released into plasma was also observed after stimulation of collagen in these patients. Platelets in the patients with normal functions contained almost normal amounts of adenine nucleotides. We discussed the relationship between platelet dysfunction and adenine nucleotides in platelets of myeloproliferative disorders and concluded that platelet dysfunction was mainly attributable to reduction of releasable ADP."}
{"id": "PMID:483251", "title": "Immunohistochemical and histochemical investigations on in vivo thrombolysis with urokinase in rabbits.", "content": "Using human urokinase in vivo thrombolysis was studied in autologous artificial thrombi in the pulmonary circulation of rabbits by immunofluorescence and Todd's fibrinolysis autography techniques. As compared to the control thrombi of the untreated rabbits, a small increase of thrombolysis was found in the rabbits treated with urokinase. Urokinase fluorescence was observed in the leukocytes, but not along the fibrin fibrils in the thrombi of the rabbits treated with urokinase. By Todd's fibrinolysis autography, lytic areas were observed around aggregated leukocytes in the thrombi of the rabbits treated with urokinase. The small lysis of autologous artificial thrombi of rabbits by human urokinase may be caused by (1) low affinity of human urokinase for rabbit plasminogen, (2) weak adsorption of human urokinase to rabbit fibrin, (3) phagocytosis of human urokinase by leukocytes and (4) high level of antifibrinolytic activity of rabbit plasma.", "contents": "Immunohistochemical and histochemical investigations on in vivo thrombolysis with urokinase in rabbits. Using human urokinase in vivo thrombolysis was studied in autologous artificial thrombi in the pulmonary circulation of rabbits by immunofluorescence and Todd's fibrinolysis autography techniques. As compared to the control thrombi of the untreated rabbits, a small increase of thrombolysis was found in the rabbits treated with urokinase. Urokinase fluorescence was observed in the leukocytes, but not along the fibrin fibrils in the thrombi of the rabbits treated with urokinase. By Todd's fibrinolysis autography, lytic areas were observed around aggregated leukocytes in the thrombi of the rabbits treated with urokinase. The small lysis of autologous artificial thrombi of rabbits by human urokinase may be caused by (1) low affinity of human urokinase for rabbit plasminogen, (2) weak adsorption of human urokinase to rabbit fibrin, (3) phagocytosis of human urokinase by leukocytes and (4) high level of antifibrinolytic activity of rabbit plasma."}
{"id": "PMID:483300", "title": "A case report of the immunodysplasia syndrome and heavy chain disease associated with subacute bacterial endocarditis.", "content": "A 36-year-old man was admitted to Saitama Medical School Hospital, because of a remittent fever which had continued for approximately 6 months, hepatosplenomegaly and lymphadenopathy. He had direct Coombs' test positive auto-immune hemolytic anemia associted with subacute bacterial endocarditis (SBE). The lymphnode demonstrated focal diffuse proliferation of immunoblasts and arborizing vessels with a few small germinal centers, which resembled histological features of the immunoblastic lymphadenopathy. The immunochemical analysis revealed the presence of free IgG Fc fragments in serum. From the above results the patient was diagnosed as immunodysplasia syndrome (IDS) and heavy chain disease (HCD) associated with SBE. It was suggested that the chronic antigenic stimulation due to SBE might have some role in the mechanism of the development of the IDS and HCD in our patient.", "contents": "A case report of the immunodysplasia syndrome and heavy chain disease associated with subacute bacterial endocarditis. A 36-year-old man was admitted to Saitama Medical School Hospital, because of a remittent fever which had continued for approximately 6 months, hepatosplenomegaly and lymphadenopathy. He had direct Coombs' test positive auto-immune hemolytic anemia associted with subacute bacterial endocarditis (SBE). The lymphnode demonstrated focal diffuse proliferation of immunoblasts and arborizing vessels with a few small germinal centers, which resembled histological features of the immunoblastic lymphadenopathy. The immunochemical analysis revealed the presence of free IgG Fc fragments in serum. From the above results the patient was diagnosed as immunodysplasia syndrome (IDS) and heavy chain disease (HCD) associated with SBE. It was suggested that the chronic antigenic stimulation due to SBE might have some role in the mechanism of the development of the IDS and HCD in our patient."}
{"id": "PMID:483301", "title": "Chronic hypernatremia associated with holoprosencephaly.", "content": "Two patients ( 1 7/12-year-old and 1 11/12-year-old girls) with chronic hypernatremia were studied. Neuroradiological findings and mildine facial defects  showed characteristic features of holoprosencephaly. Water deprivation tests showed clear evidence of antidiuretic hormone (ADH) secretion. The responses to hypertonic saline infusion and acute water loading were abnormal. In one case, the impaired osmotic regulation of ADH secretion was demonstrated by measuring urinary ADH by a radioimmunoassay. In this case, the volume regulation of ADH secretion seemed to be also incomplete since chronic water loading for a period of six days induced water retension.", "contents": "Chronic hypernatremia associated with holoprosencephaly. Two patients ( 1 7/12-year-old and 1 11/12-year-old girls) with chronic hypernatremia were studied. Neuroradiological findings and mildine facial defects  showed characteristic features of holoprosencephaly. Water deprivation tests showed clear evidence of antidiuretic hormone (ADH) secretion. The responses to hypertonic saline infusion and acute water loading were abnormal. In one case, the impaired osmotic regulation of ADH secretion was demonstrated by measuring urinary ADH by a radioimmunoassay. In this case, the volume regulation of ADH secretion seemed to be also incomplete since chronic water loading for a period of six days induced water retension."}
{"id": "PMID:483302", "title": "Effect of aminophylline on regional perfusion distribution in the lungs.", "content": "Using ten normal dogs, the right upper lobe of the lung was isolated in vivo by a balloon catheter and was artificially ventilated with nitrogen, air, 60% oxygen in nitrogen, and 60% oxygen and 20% carbon dioxide in nitrogen, while the rest of the lungs maintained a spontaneous breathing of ambient air. Aminophylline did not show a vasodilating action under severe alveolar hypoxia (PAO2: ca. 40 mmHg); on the contrary, it seemed to potentiate hypoxic pulmonary vasoconstriction. When the regional alveolar oxygen tension became less hypoxic (PAO2: ca. 70 mmHg) or higher than that in the rest of the lungs which spontaneously breathed ambient air, aminophylline showed a definite vasodilating action. Aminophylline also showed a vasodilating action in alveolar hypercapnia in the presence of alveolar hyperoxia.", "contents": "Effect of aminophylline on regional perfusion distribution in the lungs. Using ten normal dogs, the right upper lobe of the lung was isolated in vivo by a balloon catheter and was artificially ventilated with nitrogen, air, 60% oxygen in nitrogen, and 60% oxygen and 20% carbon dioxide in nitrogen, while the rest of the lungs maintained a spontaneous breathing of ambient air. Aminophylline did not show a vasodilating action under severe alveolar hypoxia (PAO2: ca. 40 mmHg); on the contrary, it seemed to potentiate hypoxic pulmonary vasoconstriction. When the regional alveolar oxygen tension became less hypoxic (PAO2: ca. 70 mmHg) or higher than that in the rest of the lungs which spontaneously breathed ambient air, aminophylline showed a definite vasodilating action. Aminophylline also showed a vasodilating action in alveolar hypercapnia in the presence of alveolar hyperoxia."}
{"id": "PMID:483303", "title": "Effects of pantetheine on cholesteryl ester synthesis in the arterial wall of rats on high cholesterol diet.", "content": "Increase of acyl-CoA synthesis was observed when extracts of rat arterial wall were incubated with pantetheine [D-bis-(N-pantothenyl-beta-aminoethyl)-disulfide]. Cholesteryl ester synthesis from palmitate in the arterial wall extract in vitro was higher with arteries from rats on high cholesterol diet than with those from rats on normal diet, but the synthesis was reduced in the arteries of rats on high cholesterol diet with pantetheine. Triglyceride synthesis was higher with arterial wall extracts of rats on high cholesterol diet than with preparations from rats on normal diet and was not reduced with those of rats on high cholesterol diet plus pantetheine. The value of the effects of pantetheine on lipid metabolism in the prevention of atherosclerosis is pointed out.", "contents": "Effects of pantetheine on cholesteryl ester synthesis in the arterial wall of rats on high cholesterol diet. Increase of acyl-CoA synthesis was observed when extracts of rat arterial wall were incubated with pantetheine [D-bis-(N-pantothenyl-beta-aminoethyl)-disulfide]. Cholesteryl ester synthesis from palmitate in the arterial wall extract in vitro was higher with arteries from rats on high cholesterol diet than with those from rats on normal diet, but the synthesis was reduced in the arteries of rats on high cholesterol diet with pantetheine. Triglyceride synthesis was higher with arterial wall extracts of rats on high cholesterol diet than with preparations from rats on normal diet and was not reduced with those of rats on high cholesterol diet plus pantetheine. The value of the effects of pantetheine on lipid metabolism in the prevention of atherosclerosis is pointed out."}
{"id": "PMID:483304", "title": "One case of Burkitt's tumor.", "content": "A 15-year-old Japanese female with complaints of abdominal tumor and exophthalmos underwent surgery. Histologically, the tumor showed a starry-sky pattern with the macrophages interspersed among undifferentiated lymphoblastoid cells. Considering the clinical aspects, distribution of tumor deposits, and cytological findings, the tumor was diagnosed as Burkitt's tumor. The patient expired on the 42nd day of hospitalization due to general dissemination.", "contents": "One case of Burkitt's tumor. A 15-year-old Japanese female with complaints of abdominal tumor and exophthalmos underwent surgery. Histologically, the tumor showed a starry-sky pattern with the macrophages interspersed among undifferentiated lymphoblastoid cells. Considering the clinical aspects, distribution of tumor deposits, and cytological findings, the tumor was diagnosed as Burkitt's tumor. The patient expired on the 42nd day of hospitalization due to general dissemination."}
{"id": "PMID:483305", "title": "Phosphatidylcholine and phosphatidylinositol exchange proteins in rat liver.", "content": "The phospholipid exchange proteins in rat liver that stimulate the transfer of phosphatidylcholine and phosphatidylinositol between membranes were separated into three fractions and partially purified by acid pH precipitation, gel filtration on Sephadex G-75, and ionexchange chromatography on DEAE-cellulose and CM-cellulose. Throughout the steps of the purification, both the phosphatidylcholine exchange activity and the phosphatidylinositol exchange activity were measured by a liposome-liposome assay system, which used concanavalin A in the separation of donor and acceptor liposomes. One of the fractions was purified 172-fold and stimulated the phosphatidylcholine exchange but not the phosphatidylinositol exchange. The other two fractions were active in the stimulation of the phosphatidylinositol exchange as well as the phosphatidylcholine exchange and were purified 62-fold and 58-fold over the cell supernatant fraction with respect to the phosphatiylinsitol exchange activity. These two fractions stimulated the transfer of phosphatidylinositol from donor liposomes to acceptor liposomes initially deficient in this phospholipid.", "contents": "Phosphatidylcholine and phosphatidylinositol exchange proteins in rat liver. The phospholipid exchange proteins in rat liver that stimulate the transfer of phosphatidylcholine and phosphatidylinositol between membranes were separated into three fractions and partially purified by acid pH precipitation, gel filtration on Sephadex G-75, and ionexchange chromatography on DEAE-cellulose and CM-cellulose. Throughout the steps of the purification, both the phosphatidylcholine exchange activity and the phosphatidylinositol exchange activity were measured by a liposome-liposome assay system, which used concanavalin A in the separation of donor and acceptor liposomes. One of the fractions was purified 172-fold and stimulated the phosphatidylcholine exchange but not the phosphatidylinositol exchange. The other two fractions were active in the stimulation of the phosphatidylinositol exchange as well as the phosphatidylcholine exchange and were purified 62-fold and 58-fold over the cell supernatant fraction with respect to the phosphatiylinsitol exchange activity. These two fractions stimulated the transfer of phosphatidylinositol from donor liposomes to acceptor liposomes initially deficient in this phospholipid."}
{"id": "PMID:483306", "title": "Normal cardiac output in relation to age and body size.", "content": "The resting cardiac output was measured in 151 healthy persons (109 males and 42 females) of 4 to 78 years of age by the earpiece dye-dilution method by using an automatic calibration device of dye concentration. Cardiac index had a significantly inverse correlation to age in 105 cases with the age higher than 20 years: r=-0.491 and y=-4.874-0.023x, where y is cardiac index (liters/min/m2) and x is age (years). Stroke index also decreased linearly by aging throughout all ages: r=-0.426 and y=71.0-0.26 x, where x is stroke index (ml/m2) and x is age. There is no significant difference between males and females. A significant difference was found between the young group below 14 years of age and the other group older than 15 years when cardiac output was correlated to body sizes such as height, weight and surface area. The former which is corresponding to the growth age showed significantly higher cardiac output than the latter. From these results, age-matched normal predicted values were presented. It is suggested that the measured cardiac index and stroke index should be taken as abnormal if those values deviate from the normal value by 30% or more.", "contents": "Normal cardiac output in relation to age and body size. The resting cardiac output was measured in 151 healthy persons (109 males and 42 females) of 4 to 78 years of age by the earpiece dye-dilution method by using an automatic calibration device of dye concentration. Cardiac index had a significantly inverse correlation to age in 105 cases with the age higher than 20 years: r=-0.491 and y=-4.874-0.023x, where y is cardiac index (liters/min/m2) and x is age (years). Stroke index also decreased linearly by aging throughout all ages: r=-0.426 and y=71.0-0.26 x, where x is stroke index (ml/m2) and x is age. There is no significant difference between males and females. A significant difference was found between the young group below 14 years of age and the other group older than 15 years when cardiac output was correlated to body sizes such as height, weight and surface area. The former which is corresponding to the growth age showed significantly higher cardiac output than the latter. From these results, age-matched normal predicted values were presented. It is suggested that the measured cardiac index and stroke index should be taken as abnormal if those values deviate from the normal value by 30% or more."}
{"id": "PMID:483307", "title": "Nasopharyngeal malignant lymphoma: clinico-pathologic studies.", "content": "Forty-eight cases of malignant lymphoma in the nasopharyngeal regions collected from the file of the Department of Pathology, Tohoku University Hospital, over 10 years period were reviewed from the clinico-pathologic standpoint. Males outnumbered females in a ratio of 3.3 to 1. The age distribution ranged from 13 to 83 years, and the mean age was 51 years. A nasopharyngeal malignant lymphoma without lymph node involvement seemed to be a highly curable tumor by irradiation, showing 10 out of 12 survived 5 years or more. On the contrary, almost all the patients with an involvement of the cervical lymph nodes revealed early recurrences and short survivals. No cases of well-differentiated lymphocytic lymphoma or Hodgkin's disease were found in the series of the present study.", "contents": "Nasopharyngeal malignant lymphoma: clinico-pathologic studies. Forty-eight cases of malignant lymphoma in the nasopharyngeal regions collected from the file of the Department of Pathology, Tohoku University Hospital, over 10 years period were reviewed from the clinico-pathologic standpoint. Males outnumbered females in a ratio of 3.3 to 1. The age distribution ranged from 13 to 83 years, and the mean age was 51 years. A nasopharyngeal malignant lymphoma without lymph node involvement seemed to be a highly curable tumor by irradiation, showing 10 out of 12 survived 5 years or more. On the contrary, almost all the patients with an involvement of the cervical lymph nodes revealed early recurrences and short survivals. No cases of well-differentiated lymphocytic lymphoma or Hodgkin's disease were found in the series of the present study."}
{"id": "PMID:483308", "title": "The effect of non-narcotic analgesic, tramadol, on cardiac contractility in dog.", "content": "The effect of a non-narcotic analgesic, tramadol, 5 mg/kg i.v. on cardiac contractility was studied in intact dog. The maximal intrinsic velocity of contraction of the ventricle, dP/dt/K P at P=O(Vmax) which is a most sensitive and reliable index of cardiac contractility, was decreased significantly 5 and 10 min after the injection, suggesting that the drug is a mild myocardial depressant at this dose and by this route.", "contents": "The effect of non-narcotic analgesic, tramadol, on cardiac contractility in dog. The effect of a non-narcotic analgesic, tramadol, 5 mg/kg i.v. on cardiac contractility was studied in intact dog. The maximal intrinsic velocity of contraction of the ventricle, dP/dt/K P at P=O(Vmax) which is a most sensitive and reliable index of cardiac contractility, was decreased significantly 5 and 10 min after the injection, suggesting that the drug is a mild myocardial depressant at this dose and by this route."}
{"id": "PMID:483320", "title": "[Direct determination of clonazepam (Rivotril) and 7-amino clonazepam in plasma by gas-chromatography (author's transl)].", "content": "A method is developed for direct gas-chromatographic determination of clonazepam (Rivotril) and its main metabolite, 7-amino clonazepam, in plasma, using desmethylflunitrazepam as internal standard. Following selective extraction, the benzodiazepines are analyzed by gas-chromatography, with a glass column filled with 3% OV17 on Gas Chrom Q and 63Ni electron capture detector. The procedure, which requires neither hydrolysis nor derivatisation, has a good selectivity. The sensitivity is 5 ng/ml of plasma for a valid quantitative determination. We have to improve this limit for fine pharmacokinetic studies, but the method is already available for therapeutic and pharmacovigilance controls. It is also suitable for diagnostic of eventual overdosing or poisoning, based on plasma or urine analysis.", "contents": "[Direct determination of clonazepam (Rivotril) and 7-amino clonazepam in plasma by gas-chromatography (author's transl)]. A method is developed for direct gas-chromatographic determination of clonazepam (Rivotril) and its main metabolite, 7-amino clonazepam, in plasma, using desmethylflunitrazepam as internal standard. Following selective extraction, the benzodiazepines are analyzed by gas-chromatography, with a glass column filled with 3% OV17 on Gas Chrom Q and 63Ni electron capture detector. The procedure, which requires neither hydrolysis nor derivatisation, has a good selectivity. The sensitivity is 5 ng/ml of plasma for a valid quantitative determination. We have to improve this limit for fine pharmacokinetic studies, but the method is already available for therapeutic and pharmacovigilance controls. It is also suitable for diagnostic of eventual overdosing or poisoning, based on plasma or urine analysis."}
{"id": "PMID:483321", "title": "[Influence of dental amalgams on the concentration of mercury and silver in biological fluids and hair (author's transl)].", "content": "The influence of the extraction and fixing of dental amalgams on the metallic concentrations of different biological samples was studied by atomic absorption spectrometry on a subject. The levels of mercury obviously increased in urine, saliva and slightly in blood, with a maximum on the second or third day after the intervention; on the other hand, in the hair, the increase was very low even at the end of 40 days. The urinary and salivary kinetics of silver were practically similar to the ones of mercury. The determination of silver in these fluids could reveal with more specificity the part of dental amalgams on the increase of the metallic biological concentrations. A study with 25 other subjects carrying amalgams permitted to put in evidence some factors facilitating a slight increase of the mercury contents of the saliva, urine and blood.", "contents": "[Influence of dental amalgams on the concentration of mercury and silver in biological fluids and hair (author's transl)]. The influence of the extraction and fixing of dental amalgams on the metallic concentrations of different biological samples was studied by atomic absorption spectrometry on a subject. The levels of mercury obviously increased in urine, saliva and slightly in blood, with a maximum on the second or third day after the intervention; on the other hand, in the hair, the increase was very low even at the end of 40 days. The urinary and salivary kinetics of silver were practically similar to the ones of mercury. The determination of silver in these fluids could reveal with more specificity the part of dental amalgams on the increase of the metallic biological concentrations. A study with 25 other subjects carrying amalgams permitted to put in evidence some factors facilitating a slight increase of the mercury contents of the saliva, urine and blood."}
{"id": "PMID:483322", "title": "[Neurological toxicity of colistine methane sulfonate, about a case (author's transl)].", "content": "The authors report a case to call attention on a Neurological Accident happening in a patient with normal renal functions, after administration of a single and strong dose of Colistine. The authors report their case and make a review of the literature on the frequency of these isolated neurological (accidents) and their supposed (pathogenesis).", "contents": "[Neurological toxicity of colistine methane sulfonate, about a case (author's transl)]. The authors report a case to call attention on a Neurological Accident happening in a patient with normal renal functions, after administration of a single and strong dose of Colistine. The authors report their case and make a review of the literature on the frequency of these isolated neurological (accidents) and their supposed (pathogenesis)."}
{"id": "PMID:483323", "title": "[A simple and rapid method for blood gas extraction for the measurement of carbon monoxide by infrared spectrometry (author's transl)].", "content": "Carbon monoxide is extracted along with other blood gases, in a plexiglass syringe, at room temperature, by vacuum in the presence of phosphoric acid. The gases liberated are transferred to another plexiglass syringe where they are diluted with air. The carbon monoxide content of this gazeous mixture is determined by infrared spectrometry. This simple and rapid extraction procedure provides reliable results and is performed with robust equipment which is easy to clean and maintain.", "contents": "[A simple and rapid method for blood gas extraction for the measurement of carbon monoxide by infrared spectrometry (author's transl)]. Carbon monoxide is extracted along with other blood gases, in a plexiglass syringe, at room temperature, by vacuum in the presence of phosphoric acid. The gases liberated are transferred to another plexiglass syringe where they are diluted with air. The carbon monoxide content of this gazeous mixture is determined by infrared spectrometry. This simple and rapid extraction procedure provides reliable results and is performed with robust equipment which is easy to clean and maintain."}
{"id": "PMID:483333", "title": "Comparison of two rapid cortisol radioimmunoassays for use in the fetal sheep.", "content": "Cortisol radioimmunoassays (RIA's) utilizing highly specific antisera combined with a simple ethanol protein precipitation procedure (ETOH-PPT) are widely utilized to measure cortisol in human plasma. This same type of RIA has been assumed specific for measurement of cortisol in the plasma of several different species of experimental animals. In order to test this assumption as applied to fetal ovine plasma, we compared an ETOH-PPT cortisol RIA with another rapid cortisol assay which utilizes a dichloromethane extraction (DM-E) step. The DM-E assay in turn was compared with a chromatographic assay previously shown to be highly specific for measurement of fetal plasma cortisol in this species. Fetal ovine plasma cortisol concentrations determined by the DM-E method were nearly identical to the concentrations obtained by the specific chromatographic RIA procedure. On the other hand, the ETOH-PPT RIA grossly overestimated cortisol concentrations when compared with the DM-E RIA. While the rapid DM-E RIA appears to be suitable for use in fetal ovine plasma, the widely used ETOH-PPT RIA yields spuriously high and unpredictable values and must be considered unreliable. These comparisons demonstrate the need for careful reassessment of steroid assays prior to their application in experimental animals even though they have been previously documented as specific in human plasma.", "contents": "Comparison of two rapid cortisol radioimmunoassays for use in the fetal sheep. Cortisol radioimmunoassays (RIA's) utilizing highly specific antisera combined with a simple ethanol protein precipitation procedure (ETOH-PPT) are widely utilized to measure cortisol in human plasma. This same type of RIA has been assumed specific for measurement of cortisol in the plasma of several different species of experimental animals. In order to test this assumption as applied to fetal ovine plasma, we compared an ETOH-PPT cortisol RIA with another rapid cortisol assay which utilizes a dichloromethane extraction (DM-E) step. The DM-E assay in turn was compared with a chromatographic assay previously shown to be highly specific for measurement of fetal plasma cortisol in this species. Fetal ovine plasma cortisol concentrations determined by the DM-E method were nearly identical to the concentrations obtained by the specific chromatographic RIA procedure. On the other hand, the ETOH-PPT RIA grossly overestimated cortisol concentrations when compared with the DM-E RIA. While the rapid DM-E RIA appears to be suitable for use in fetal ovine plasma, the widely used ETOH-PPT RIA yields spuriously high and unpredictable values and must be considered unreliable. These comparisons demonstrate the need for careful reassessment of steroid assays prior to their application in experimental animals even though they have been previously documented as specific in human plasma."}
{"id": "PMID:483335", "title": "Hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and cholesterol 7 alpha-hydroxylase activity in neonatal guinea pig.", "content": "Optimal assay conditions for hepatic HMG-CoA reducatase activity and cholesterol 7 alpha-hydroxylase activity in the guinea pig were determined. These two enzyme activities were studied in the liver of newborn guinea pigs during the first three postnatal weeks. Hepatic HMG-CoA reductase activity of neonatal guinea pigs was similar to that of adult animals. However, cholesterol 7 alpha-hydroxylase activity of newborns was about one-third of that in adult guinea pigs. This finding suggests that the system for bile acid synthesis in the neonatal guinea pigs is underdeveloped.", "contents": "Hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and cholesterol 7 alpha-hydroxylase activity in neonatal guinea pig. Optimal assay conditions for hepatic HMG-CoA reducatase activity and cholesterol 7 alpha-hydroxylase activity in the guinea pig were determined. These two enzyme activities were studied in the liver of newborn guinea pigs during the first three postnatal weeks. Hepatic HMG-CoA reductase activity of neonatal guinea pigs was similar to that of adult animals. However, cholesterol 7 alpha-hydroxylase activity of newborns was about one-third of that in adult guinea pigs. This finding suggests that the system for bile acid synthesis in the neonatal guinea pigs is underdeveloped."}
{"id": "PMID:483336", "title": "Isolation and partial synthesis of a new metabolite of medroxyrogesterone acetate.", "content": "3 alpha-Hydroxy-17-acetoxy-6 alpha-methyl-5 beta-pregnan-20-one (IIIa) has been isolated from urine of patients receiving medroxyprogesterone acetate (MPA). It was characterized by partial synthesis from MPA by catalytic reduction with palladium-charcoal to 17-acetoxy-6 alpha-methyl-5 beta-pregnan-3,20-dione (IV) and reduction of the latter with sodium borohydride. The isolation of 6 beta, 17,21-trihydroxy-6 alpha-methyl-pregn-4-ene-3,20-dione (IIc) is reported for the first time. The 17- and 21-monoacetates of this compound have been isolated and characterized earlier by other investigators. 7 alpha-3H-Medroxyprogesterone acetate was administered to 4 subjects by intravenous and intramuscular injections and by mouth. The ring A saturated metabolite IIIa was excreted in 0.1% to 4.0% of the administered dose; the highest excretion was after the intravenous dose and lowest after oral ingestion. 6 beta, 17,21-Trihydroxy-6 alpha-methylpregn-4-ene-3,20-dione (IIc) and its 17- and 21-monoacetates were excreted in about 5% of the doses in all subjects. No increase in 6 beta-hydroxylation was observed in the patient treated with o,p'-DDD,2,2-bis(2-chlorophenyl, 4'-chlorophenyl)-l,1-dichloroethane.", "contents": "Isolation and partial synthesis of a new metabolite of medroxyrogesterone acetate. 3 alpha-Hydroxy-17-acetoxy-6 alpha-methyl-5 beta-pregnan-20-one (IIIa) has been isolated from urine of patients receiving medroxyprogesterone acetate (MPA). It was characterized by partial synthesis from MPA by catalytic reduction with palladium-charcoal to 17-acetoxy-6 alpha-methyl-5 beta-pregnan-3,20-dione (IV) and reduction of the latter with sodium borohydride. The isolation of 6 beta, 17,21-trihydroxy-6 alpha-methyl-pregn-4-ene-3,20-dione (IIc) is reported for the first time. The 17- and 21-monoacetates of this compound have been isolated and characterized earlier by other investigators. 7 alpha-3H-Medroxyprogesterone acetate was administered to 4 subjects by intravenous and intramuscular injections and by mouth. The ring A saturated metabolite IIIa was excreted in 0.1% to 4.0% of the administered dose; the highest excretion was after the intravenous dose and lowest after oral ingestion. 6 beta, 17,21-Trihydroxy-6 alpha-methylpregn-4-ene-3,20-dione (IIc) and its 17- and 21-monoacetates were excreted in about 5% of the doses in all subjects. No increase in 6 beta-hydroxylation was observed in the patient treated with o,p'-DDD,2,2-bis(2-chlorophenyl, 4'-chlorophenyl)-l,1-dichloroethane."}
{"id": "PMID:483337", "title": "Bile acid sulfates in serum bile acids determination.", "content": "Some bile acid sulfates were synthesized and characterized. The configuration of sulfate groups at C-3, C-7 and C-12 positions was confirmed by Nuclear Magnetic Resonance analysis. These sulfates were utilized in a study of their chemical behaviour in different analytical procedures currently used for serum bile acids determination. Procedures for bile acids extraction from serum with ethanol or Amberlite XAD-2 result in an important loss of the most polar sulfated bile acids. Complete separation of unsulfated from sulfated bile acids on Sephadex LH-20 is not achieved when deconjugation of the most polar bile acid sulfate is slow but does not produce artifacts. Enzymatic determination of bile acids gives positive response with some bile acid sulfates. The current procedures of serum bile acids determination are discussed in consideration of these results.", "contents": "Bile acid sulfates in serum bile acids determination. Some bile acid sulfates were synthesized and characterized. The configuration of sulfate groups at C-3, C-7 and C-12 positions was confirmed by Nuclear Magnetic Resonance analysis. These sulfates were utilized in a study of their chemical behaviour in different analytical procedures currently used for serum bile acids determination. Procedures for bile acids extraction from serum with ethanol or Amberlite XAD-2 result in an important loss of the most polar sulfated bile acids. Complete separation of unsulfated from sulfated bile acids on Sephadex LH-20 is not achieved when deconjugation of the most polar bile acid sulfate is slow but does not produce artifacts. Enzymatic determination of bile acids gives positive response with some bile acid sulfates. The current procedures of serum bile acids determination are discussed in consideration of these results."}
{"id": "PMID:483338", "title": "The binding of sterol sulfates to hamster spermatozoa.", "content": "Spermatozoa obtained from the cauda epididymidis possess twice the ability to take up sterol sulfates in vitro when compared to sermatozoa obtained from the caput. This would suggest that a modification of the membrane composition of the spermatozoa occurs during passage through the epididymis. Free sterols are taken up in a similar pattern. Radioautographic studies reveal that, for sterol sulfates, this uptake occurs selectively in the regions of the head and mid-piece of the spermatozoa whereas the free sterols are distributed evenly throughout the length of the spermatozoa. The binding of sterol sulfates to spermatozoa appears to involve sites that are unsaturable. The possibility exists that sterol sulfates, previously implicated in membrane stabilization, may play a similar role in spermatozoa.", "contents": "The binding of sterol sulfates to hamster spermatozoa. Spermatozoa obtained from the cauda epididymidis possess twice the ability to take up sterol sulfates in vitro when compared to sermatozoa obtained from the caput. This would suggest that a modification of the membrane composition of the spermatozoa occurs during passage through the epididymis. Free sterols are taken up in a similar pattern. Radioautographic studies reveal that, for sterol sulfates, this uptake occurs selectively in the regions of the head and mid-piece of the spermatozoa whereas the free sterols are distributed evenly throughout the length of the spermatozoa. The binding of sterol sulfates to spermatozoa appears to involve sites that are unsaturable. The possibility exists that sterol sulfates, previously implicated in membrane stabilization, may play a similar role in spermatozoa."}
{"id": "PMID:483339", "title": "Evaluating acceptance strategies for timing of postpartum contraception.", "content": "What is the best time after childbirth to accept contraception? The problem is to reduce overlap with postpartum anovulation, during which contraception is redundant, but simultaneously to reduce the proportion of women conceiving before contraception is inaugurated. Efficiencies of three classes of acceptance strategies at resolving this dilemma are investigated. Particular attention is paid to the postpartum acceptance rule because of its special administrative advantages. Its efficiency relative to those of other rules is found to be enhanced by conditions of short postpartum anovulation, a high proportion of ovulatory rather than anovulatory first menstrual cycles, a high level of natural fecundability, and, especially, a high level of contraceptive continuation.", "contents": "Evaluating acceptance strategies for timing of postpartum contraception. What is the best time after childbirth to accept contraception? The problem is to reduce overlap with postpartum anovulation, during which contraception is redundant, but simultaneously to reduce the proportion of women conceiving before contraception is inaugurated. Efficiencies of three classes of acceptance strategies at resolving this dilemma are investigated. Particular attention is paid to the postpartum acceptance rule because of its special administrative advantages. Its efficiency relative to those of other rules is found to be enhanced by conditions of short postpartum anovulation, a high proportion of ovulatory rather than anovulatory first menstrual cycles, a high level of natural fecundability, and, especially, a high level of contraceptive continuation."}
{"id": "PMID:483340", "title": "Survey of abortion providers in Seoul, Korea.", "content": "A survey of abortion providers in Seoul in late 1977 reveals a threefold increase in the rate of abortion and a greater than threefold rise in the ratio of abortions to live births since 1970. The survey findings also show that the overwhelming proportion of these abortions are performed in private clinics, by obstetrician-gynecologists, during the first trimester. The Seoul figures, which are similar to those of Romania and are higher than those of Japan in the early 1960s, indicate that abortion has contributed substantially to Korea's reduced fertility.", "contents": "Survey of abortion providers in Seoul, Korea. A survey of abortion providers in Seoul in late 1977 reveals a threefold increase in the rate of abortion and a greater than threefold rise in the ratio of abortions to live births since 1970. The survey findings also show that the overwhelming proportion of these abortions are performed in private clinics, by obstetrician-gynecologists, during the first trimester. The Seoul figures, which are similar to those of Romania and are higher than those of Japan in the early 1960s, indicate that abortion has contributed substantially to Korea's reduced fertility."}
{"id": "PMID:483341", "title": "Past and current contraceptive use in Pakistan.", "content": "Despite a vigorous national family planning program and such innovations as the Continuous Motivation System, there has been little change in contraceptive use in Pakistan in recent years. Data from the 1968-69 National Impact Survey and the 1975 World Fertility Survey for Pakistan show that, while use rates increased by a small amount in urban areas, the rates in rural areas seem to have declined. Even in urban areas contraceptive use is low in comparison with other developing countries that began family planning programs around the same time. Although this is probably due to differences in economic development, further investigation of program structure and inputs is recommended.", "contents": "Past and current contraceptive use in Pakistan. Despite a vigorous national family planning program and such innovations as the Continuous Motivation System, there has been little change in contraceptive use in Pakistan in recent years. Data from the 1968-69 National Impact Survey and the 1975 World Fertility Survey for Pakistan show that, while use rates increased by a small amount in urban areas, the rates in rural areas seem to have declined. Even in urban areas contraceptive use is low in comparison with other developing countries that began family planning programs around the same time. Although this is probably due to differences in economic development, further investigation of program structure and inputs is recommended."}
{"id": "PMID:483352", "title": "Suicide in pregnancy: the Hedda Gabler syndrome.", "content": "A review of forty-seven suicides in women of child-bearing age revealed that two women were pregnant, two were within the first year post-partum and two falsely believed themselves pregnant. The search revealed two additional pregnancy-associated suicides which had not been reported as suicide. Previous studies asserting that pregnancy protects against suicide would seem to be challenged by these data. Pregnancy-related suicides are similar to each other and to the literary suicide of Hedda Gabler and fit into a continuum of pregnancy-related disorders.", "contents": "Suicide in pregnancy: the Hedda Gabler syndrome. A review of forty-seven suicides in women of child-bearing age revealed that two women were pregnant, two were within the first year post-partum and two falsely believed themselves pregnant. The search revealed two additional pregnancy-associated suicides which had not been reported as suicide. Previous studies asserting that pregnancy protects against suicide would seem to be challenged by these data. Pregnancy-related suicides are similar to each other and to the literary suicide of Hedda Gabler and fit into a continuum of pregnancy-related disorders."}
{"id": "PMID:483353", "title": "Dependent children and suicide of married parents.", "content": "In an attempt to analyze the relatively neglected relationship between the presence of dependent children and suicide of married parents, the suicides of both married and single persons in the Chicago-Cook County region for 1970 and 1974 were examined. Single suicides and married suicides with dependent children were compared to similar groups in the general population. It was found that while marrieds with dependent children experienced the lowest average suicide rate, they also had a larger mean number of children than the population as a whole and exhibited a tendency for having children at a very young age or children at an older age in life. These findings contradict contemporary notions that an increasing number of children in a marriage tend to inhibit suicide potential. It appears that the relationship between marital suicide and dependent children is far more complex than previously indicated and requires further research for elaboration.", "contents": "Dependent children and suicide of married parents. In an attempt to analyze the relatively neglected relationship between the presence of dependent children and suicide of married parents, the suicides of both married and single persons in the Chicago-Cook County region for 1970 and 1974 were examined. Single suicides and married suicides with dependent children were compared to similar groups in the general population. It was found that while marrieds with dependent children experienced the lowest average suicide rate, they also had a larger mean number of children than the population as a whole and exhibited a tendency for having children at a very young age or children at an older age in life. These findings contradict contemporary notions that an increasing number of children in a marriage tend to inhibit suicide potential. It appears that the relationship between marital suicide and dependent children is far more complex than previously indicated and requires further research for elaboration."}
{"id": "PMID:483354", "title": "Some sociopsychological factors in attempted suicide among urban black males.", "content": "This study tests three hypotheses relating to suicidal behavior among urban black males: that black consciousness and group cohesiveness will be lower and depression will be higher. Inner-city, young adult black males with a suicide attempt in the last 6 months, and matched controls were examined. Hypothesis was one supported; the other two received partial support. The validity of the theory of black self-hatred as the basic cause of suicidal behavior among blacks is questioned. Atwo-factor theory of suicide, focusing on forces moving the person away from suicide toward positive subgroup ties, is proposed.", "contents": "Some sociopsychological factors in attempted suicide among urban black males. This study tests three hypotheses relating to suicidal behavior among urban black males: that black consciousness and group cohesiveness will be lower and depression will be higher. Inner-city, young adult black males with a suicide attempt in the last 6 months, and matched controls were examined. Hypothesis was one supported; the other two received partial support. The validity of the theory of black self-hatred as the basic cause of suicidal behavior among blacks is questioned. Atwo-factor theory of suicide, focusing on forces moving the person away from suicide toward positive subgroup ties, is proposed."}
{"id": "PMID:483355", "title": "Cognitive aspects of suicidal behavior.", "content": "Recent efforts to quantify various aspects of suicide ideation are described. The Suicide Intent Scale, Hopelessness Scale, and the Scale for Suicide Ideation all assess components of the individual's suicidal ruminations. Hopelessness seems to account for the bulk of the relationship between depression and suicidal intent. The Scale for Suicide Ideation seems to show promise as a predictive instrument, as a dependent variable in research on treatment efficacy, and as a tool for clinical assessment of suicide potential. Implications for further research and clinical practice are discussed.", "contents": "Cognitive aspects of suicidal behavior. Recent efforts to quantify various aspects of suicide ideation are described. The Suicide Intent Scale, Hopelessness Scale, and the Scale for Suicide Ideation all assess components of the individual's suicidal ruminations. Hopelessness seems to account for the bulk of the relationship between depression and suicidal intent. The Scale for Suicide Ideation seems to show promise as a predictive instrument, as a dependent variable in research on treatment efficacy, and as a tool for clinical assessment of suicide potential. Implications for further research and clinical practice are discussed."}
{"id": "PMID:483356", "title": "Chronic callers to a suicide prevention center.", "content": "Records of 67 chronic callers to the Cleveland Suicide Prevention Centers over the past 9 years are reviewed. Of this group, 51% were diagnosed as drug or alcohol dependent. It was also noted that 4 of the 67 were known to have committed suicide and that another 37 (55%) had made suicide attempts. A surprising finding was that 47% of the group was eventually referred to an ongoing treatment resource. Agency strategies for dealing with the chronic caller are discussed.", "contents": "Chronic callers to a suicide prevention center. Records of 67 chronic callers to the Cleveland Suicide Prevention Centers over the past 9 years are reviewed. Of this group, 51% were diagnosed as drug or alcohol dependent. It was also noted that 4 of the 67 were known to have committed suicide and that another 37 (55%) had made suicide attempts. A surprising finding was that 47% of the group was eventually referred to an ongoing treatment resource. Agency strategies for dealing with the chronic caller are discussed."}
{"id": "PMID:483357", "title": "Peripheral nerve allografts. I. Distinctive histological features of nerve degeneration and immunological rejection.", "content": "Sequential sections were prepared from isografted or allografted sciatic nerves in adult female DBA/2 mice and were compared with the wallerian degeneration in the same inbred strain. Transverse sections were studied by qualitative and quantitative histology. Rejection was manifested by an important infiltration of mononuclear cells, including large pyroninophilic cells, around the grafted nerve, followed by the appearance of irregular infiltrates around the neovessels within the nerve. In parallel, the number of endoneural nuclei increased less rapidly in allografts than in isografts up to day 15 and diminished thereafter, in association with a possible reduction of neural vascularisation.", "contents": "Peripheral nerve allografts. I. Distinctive histological features of nerve degeneration and immunological rejection. Sequential sections were prepared from isografted or allografted sciatic nerves in adult female DBA/2 mice and were compared with the wallerian degeneration in the same inbred strain. Transverse sections were studied by qualitative and quantitative histology. Rejection was manifested by an important infiltration of mononuclear cells, including large pyroninophilic cells, around the grafted nerve, followed by the appearance of irregular infiltrates around the neovessels within the nerve. In parallel, the number of endoneural nuclei increased less rapidly in allografts than in isografts up to day 15 and diminished thereafter, in association with a possible reduction of neural vascularisation."}
{"id": "PMID:483358", "title": "Specific destruction of human endothelial cell monolayers by anti-DRw antisera.", "content": "Human endothelial cells were separated by collagenase digestion from the umbilical vein of newborns. The cytotoxic effect of antisera against HLA-DRw antigens was tested on monolayers of endothelial cells, using a 51Cr retention microcytotoxicity assay in the presence of rabbit complement. Of the endothelial monolayers tested, significant concordance between the typing results obtained using cord blood-derived B lymphocytes and endothelial cell monolayers was observed. A rabbit anti-human polyspecific B cell antiserum was also capable of completely lysing the endothelial monolayers tested. In addition, 51Cr-labelled dissociated endothelial cells were lysed by nonimmune peripheral blood mononuclear cells in the presence of DRw antisera having a specificity for the target endothelium.", "contents": "Specific destruction of human endothelial cell monolayers by anti-DRw antisera. Human endothelial cells were separated by collagenase digestion from the umbilical vein of newborns. The cytotoxic effect of antisera against HLA-DRw antigens was tested on monolayers of endothelial cells, using a 51Cr retention microcytotoxicity assay in the presence of rabbit complement. Of the endothelial monolayers tested, significant concordance between the typing results obtained using cord blood-derived B lymphocytes and endothelial cell monolayers was observed. A rabbit anti-human polyspecific B cell antiserum was also capable of completely lysing the endothelial monolayers tested. In addition, 51Cr-labelled dissociated endothelial cells were lysed by nonimmune peripheral blood mononuclear cells in the presence of DRw antisera having a specificity for the target endothelium."}
{"id": "PMID:483359", "title": "Pharmacological protection of rabbit kidneys from normothermic ischemia.", "content": "This study investigated the influence of the administration of pharmacological agents on the recovery of rabbit kidneys from the effects of 1 hr of situ normothermic ischemia, utilizing acute and chronic models. The agents tested included the diuretics mannitol and furosemide, the vasoactive agents phenoxybenzamine, propranolol, and dopamine, and the membrane stabilizers chlorpromazine and methylprednisolone. A beneficial effect was detected only with the diuretic agents and propranolol when given prior to the ischemic insult.", "contents": "Pharmacological protection of rabbit kidneys from normothermic ischemia. This study investigated the influence of the administration of pharmacological agents on the recovery of rabbit kidneys from the effects of 1 hr of situ normothermic ischemia, utilizing acute and chronic models. The agents tested included the diuretics mannitol and furosemide, the vasoactive agents phenoxybenzamine, propranolol, and dopamine, and the membrane stabilizers chlorpromazine and methylprednisolone. A beneficial effect was detected only with the diuretic agents and propranolol when given prior to the ischemic insult."}
{"id": "PMID:483360", "title": "Activity of syngeneic complement for revealing antibody-induced cytotoxicity against a rat hepatoma.", "content": "The cytotoxicity of tumour-bearer serum against a transplanted aminoazodye-induced rat hepatoma was revealed using normal rat serum as the source of complement as assessed using a short-term 51Cr release test. The tumour-bearing rat was not deficient in a functional complement system and direct cytolysis against hepatoma cells was demonstrated following admixture of tumour cells and serum with no additional complement. No tumour growth was observed in animals receiving subcutaneous transfer of cells treated with tumour-bearer serum, although when heat-inactivated (56 C for 60 min) or normal sera were used, there was no modification of tumour development. The present findings indicate that the tumour-bearing animal contains both antibodies and complement sufficient for tumour lysis, although the full contribution of serum-mediated cytotoxicity in imposing immunological constraints upon tumour growth remains to be elucidated.", "contents": "Activity of syngeneic complement for revealing antibody-induced cytotoxicity against a rat hepatoma. The cytotoxicity of tumour-bearer serum against a transplanted aminoazodye-induced rat hepatoma was revealed using normal rat serum as the source of complement as assessed using a short-term 51Cr release test. The tumour-bearing rat was not deficient in a functional complement system and direct cytolysis against hepatoma cells was demonstrated following admixture of tumour cells and serum with no additional complement. No tumour growth was observed in animals receiving subcutaneous transfer of cells treated with tumour-bearer serum, although when heat-inactivated (56 C for 60 min) or normal sera were used, there was no modification of tumour development. The present findings indicate that the tumour-bearing animal contains both antibodies and complement sufficient for tumour lysis, although the full contribution of serum-mediated cytotoxicity in imposing immunological constraints upon tumour growth remains to be elucidated."}
{"id": "PMID:483361", "title": "Action of complement in the lysis of mouse sarcoma cells sensitized with H-2 alloantibody.", "content": "A modified cytotoxicity assay was adapted from classical erythrocytolytic assays, in which complement components were added in sequence to antibody-sensitized cells. This assay was applied to a model system in which mouse sarcoma cells were sensitized with H-2 alloantibody. The stepwise presentation of complement components combined with the stabilization of C2 by iodine treatment considerably augmented the lytic efficiency of human complement. More generally, the techniques adopted for this study provide a new model for obtaining basic information about selective reaction steps concerned in lysis of nucleated cells by alloantibody and complement. Comparisons of the lysis of sheep erythrocytes by xenoantibody with the lysis of mouse sarcoma cells by H-2 alloantibody, in our assay system with oxidized or untreated human complement, disclosed a difference in the kinetics of C142 formation, manifest in a lag phase and a protracted tmax for the sarcoma cells. These data may suggest that multiple complement-mediated functional lesions are necessary for immune lysis of nucleated cells.", "contents": "Action of complement in the lysis of mouse sarcoma cells sensitized with H-2 alloantibody. A modified cytotoxicity assay was adapted from classical erythrocytolytic assays, in which complement components were added in sequence to antibody-sensitized cells. This assay was applied to a model system in which mouse sarcoma cells were sensitized with H-2 alloantibody. The stepwise presentation of complement components combined with the stabilization of C2 by iodine treatment considerably augmented the lytic efficiency of human complement. More generally, the techniques adopted for this study provide a new model for obtaining basic information about selective reaction steps concerned in lysis of nucleated cells by alloantibody and complement. Comparisons of the lysis of sheep erythrocytes by xenoantibody with the lysis of mouse sarcoma cells by H-2 alloantibody, in our assay system with oxidized or untreated human complement, disclosed a difference in the kinetics of C142 formation, manifest in a lag phase and a protracted tmax for the sarcoma cells. These data may suggest that multiple complement-mediated functional lesions are necessary for immune lysis of nucleated cells."}
{"id": "PMID:483363", "title": "Platelet transfusion tolerization by liver membrane treatment.", "content": "A nontoxic murine model has been developed in which sensitization to allogeneic platelet transfusions in adults can be prevented. This model is based upon allogeneic liver membrane (LM) induction of specific humoral tolerance to major histocompatibility alloantigens. In normal mice of the C3H background, syngeneic and H-2-incompatible congeneic platelets had a t1/2 = 15 +/- 3 hr; for mice of the C57BL background, the comparable t1/2 was 33 +/- 6 hr. Platelets of C3H background transfused into C57BL background recipients, and vice versa, had t1/22 midway between, 24 +/- 3 and 24 +/- 2 hr, respectively. These data suggest genetically determined variation in normal platelet survival. In passively immunized C3H background mice, the t1/2 was decreased to 10 +/- 2 hr. In C57BL background mice actively immunized i.p. with allogeneic lymphoid cells, t1/2 was decreased to 18 +/- 4 hr. When allogeneic LM was given concomitantly with the allogeneic cells, however, sensitization to foreign H-2 antigens was blocked, and survival of both C3H and C57BL background allogeneic platelets remained normal. These data demonstrate that in this free cell allograft system LM treatment is a safe and effective method for preventing adult sensitization to allogeneic platelets.", "contents": "Platelet transfusion tolerization by liver membrane treatment. A nontoxic murine model has been developed in which sensitization to allogeneic platelet transfusions in adults can be prevented. This model is based upon allogeneic liver membrane (LM) induction of specific humoral tolerance to major histocompatibility alloantigens. In normal mice of the C3H background, syngeneic and H-2-incompatible congeneic platelets had a t1/2 = 15 +/- 3 hr; for mice of the C57BL background, the comparable t1/2 was 33 +/- 6 hr. Platelets of C3H background transfused into C57BL background recipients, and vice versa, had t1/22 midway between, 24 +/- 3 and 24 +/- 2 hr, respectively. These data suggest genetically determined variation in normal platelet survival. In passively immunized C3H background mice, the t1/2 was decreased to 10 +/- 2 hr. In C57BL background mice actively immunized i.p. with allogeneic lymphoid cells, t1/2 was decreased to 18 +/- 4 hr. When allogeneic LM was given concomitantly with the allogeneic cells, however, sensitization to foreign H-2 antigens was blocked, and survival of both C3H and C57BL background allogeneic platelets remained normal. These data demonstrate that in this free cell allograft system LM treatment is a safe and effective method for preventing adult sensitization to allogeneic platelets."}
{"id": "PMID:483364", "title": "Mass control of ascariasis with single oral doses of levamisole. A controlled comparison in 3,056 subjects between three incomplete population coverages.", "content": "Ninety-, 60-, and 40-percent population coverages with levamisole 2.5 mg/kg of body weight were compared for their effects on Ascaris lumbricoides, ancylostoma, Strongyloides stercoralis, and Trichuris trichiura infections. They were shown to be effective in maintaining a reduced prevalence of A. lumbricoides in the treated subjects for 9, 6, and 3 months, respectively. Nine months after treatment, the prevalence of ascariasis was still lower than before treatment both in the levamisole and in the control subjects, regardless of the population coverage. This was probably because the egg output had been reduced. It is concluded that mass treatment with single oral doses of levamisole repeated at 3-month intervals might help control ascariasis, and that population coverages between 60 and 90% might be appropriate. No clear-cut effects against hookworms could be shown, possibly because the first follow-up examinations were performed three months after treatment. No changes in the prevalence of S. stercoralis and T. trichiura could be demonstrated. There were no adverse exp", "contents": "Mass control of ascariasis with single oral doses of levamisole. A controlled comparison in 3,056 subjects between three incomplete population coverages. Ninety-, 60-, and 40-percent population coverages with levamisole 2.5 mg/kg of body weight were compared for their effects on Ascaris lumbricoides, ancylostoma, Strongyloides stercoralis, and Trichuris trichiura infections. They were shown to be effective in maintaining a reduced prevalence of A. lumbricoides in the treated subjects for 9, 6, and 3 months, respectively. Nine months after treatment, the prevalence of ascariasis was still lower than before treatment both in the levamisole and in the control subjects, regardless of the population coverage. This was probably because the egg output had been reduced. It is concluded that mass treatment with single oral doses of levamisole repeated at 3-month intervals might help control ascariasis, and that population coverages between 60 and 90% might be appropriate. No clear-cut effects against hookworms could be shown, possibly because the first follow-up examinations were performed three months after treatment. No changes in the prevalence of S. stercoralis and T. trichiura could be demonstrated. There were no adverse exp"}
{"id": "PMID:483365", "title": "Occurrence of Toxoplasma gondii in slaughter pigs in India.", "content": "A serological and cultural investigation on the occurrence of T. gondii amongst slaughter pigs in north India has been carried out. Toxoplasma antibodies were found in the sera of 31.5% of the 178 pigs examined by the indirect haemagglutination test. Seropositivity at titres of 1:128 or more was shown by 11.8% of the animals. Isolation by mouse passage was successful in one out of the 26 tissue samples from 17 pigs. This is the first isolation of T. gondii from pigs in this country.", "contents": "Occurrence of Toxoplasma gondii in slaughter pigs in India. A serological and cultural investigation on the occurrence of T. gondii amongst slaughter pigs in north India has been carried out. Toxoplasma antibodies were found in the sera of 31.5% of the 178 pigs examined by the indirect haemagglutination test. Seropositivity at titres of 1:128 or more was shown by 11.8% of the animals. Isolation by mouse passage was successful in one out of the 26 tissue samples from 17 pigs. This is the first isolation of T. gondii from pigs in this country."}
{"id": "PMID:483366", "title": "Dengue hemorrhagic shock in the western hemisphere.", "content": "A 39-year-old man after visiting an endemic dengue area during a local outbreak developed a febrile illness complicated by skin petechiae, bleeding, shock, hemoconcentration, and death. The presumptive diagnosis of dengue was made based on hemagglutination inhibition and complement fixation titers in a single sample. The serologic evidence and ancillary laboratory findings are compatible with a secondary antibody response.", "contents": "Dengue hemorrhagic shock in the western hemisphere. A 39-year-old man after visiting an endemic dengue area during a local outbreak developed a febrile illness complicated by skin petechiae, bleeding, shock, hemoconcentration, and death. The presumptive diagnosis of dengue was made based on hemagglutination inhibition and complement fixation titers in a single sample. The serologic evidence and ancillary laboratory findings are compatible with a secondary antibody response."}
{"id": "PMID:483367", "title": "Oesophagostomiasis in man.", "content": "A case of oesophagostomiasis in an 8-years-old Africa girl is reported. The patient presented with abdominal pain and weight loss. Examination revealed multiple abdominal masses most of which were resected. The difficulties in differential diagnosis are discussed especially with reference to the need for increased awareness of this disease. The extant world literature on this subject is tabulated.", "contents": "Oesophagostomiasis in man. A case of oesophagostomiasis in an 8-years-old Africa girl is reported. The patient presented with abdominal pain and weight loss. Examination revealed multiple abdominal masses most of which were resected. The difficulties in differential diagnosis are discussed especially with reference to the need for increased awareness of this disease. The extant world literature on this subject is tabulated."}
{"id": "PMID:483368", "title": "Rehabilitation of xeropthalmic children. A follow-up study in southern India.", "content": "Two hundred and seventy-five children were admitted to the Nutrition Rehabilitation Centre (NRC), Madurai in 1975; 213 had xeropthalmia and 62 showed no eye signs. All were malnourished, the largest single group being less than half their expected weight for age. A follow-up study was made both by means of recall to the NRC and by means of house visits to children of Madurai town. Less than half the total, but 55% of town children were found. The distribution of eye signs on admission in those who were later followed-up, corresponded with that in the total. Weight improved while at the NRC and also after return home. All existing cases of corneal xerosis healed but conjunctival xerosis remained in some. Major corneal damage through ulceration, keratomalacia and scars was present in 94 children at admission, 34 of these were followed-up. Four were blind and five had severely limited vision in both eyes, but useful vision was present in one or both eyes of the other 25 (73%) children. For example, of 15 children found blind in one eye, 13 had good vision in their other. The mortality among xerophthalmic children children while at the NRC was 6.6%, and among those visited later at home was 20%. Almost all mothers questioned, said they gave their children green vegetables, but apart from this very important precept, few other dietary suggestions were followed.", "contents": "Rehabilitation of xeropthalmic children. A follow-up study in southern India. Two hundred and seventy-five children were admitted to the Nutrition Rehabilitation Centre (NRC), Madurai in 1975; 213 had xeropthalmia and 62 showed no eye signs. All were malnourished, the largest single group being less than half their expected weight for age. A follow-up study was made both by means of recall to the NRC and by means of house visits to children of Madurai town. Less than half the total, but 55% of town children were found. The distribution of eye signs on admission in those who were later followed-up, corresponded with that in the total. Weight improved while at the NRC and also after return home. All existing cases of corneal xerosis healed but conjunctival xerosis remained in some. Major corneal damage through ulceration, keratomalacia and scars was present in 94 children at admission, 34 of these were followed-up. Four were blind and five had severely limited vision in both eyes, but useful vision was present in one or both eyes of the other 25 (73%) children. For example, of 15 children found blind in one eye, 13 had good vision in their other. The mortality among xerophthalmic children children while at the NRC was 6.6%, and among those visited later at home was 20%. Almost all mothers questioned, said they gave their children green vegetables, but apart from this very important precept, few other dietary suggestions were followed."}
{"id": "PMID:483369", "title": "Factors associated with low birth weight in the population of Dar es Slaaam, Tanzania.", "content": "Factors associated with low birthweight in Dar es Salaam have been evaluated. The incidence of low birthweight is higher among female infants and infants belonging to parents of a low socioeconomic status. Primiparity, short stature of the mother, a multiple pregnancy, \"toxaemia\" of pregnancy are some of the factors influencing the growth velocity of the foetus leading to an increased indidence of small-for-dates infants. Low maternal age and antepartum haemorrhage mainly affect the duration of gestation and lead to a preponderance of preterm appropriate-for-gestational-age infants. However, in 66% of the mothers with low birthweight infants no associated maternal complication of the pregnancy could be detected.", "contents": "Factors associated with low birth weight in the population of Dar es Slaaam, Tanzania. Factors associated with low birthweight in Dar es Salaam have been evaluated. The incidence of low birthweight is higher among female infants and infants belonging to parents of a low socioeconomic status. Primiparity, short stature of the mother, a multiple pregnancy, \"toxaemia\" of pregnancy are some of the factors influencing the growth velocity of the foetus leading to an increased indidence of small-for-dates infants. Low maternal age and antepartum haemorrhage mainly affect the duration of gestation and lead to a preponderance of preterm appropriate-for-gestational-age infants. However, in 66% of the mothers with low birthweight infants no associated maternal complication of the pregnancy could be detected."}
{"id": "PMID:483370", "title": "Pulmonary tuberculosis in childhood. An analysis of 263 cases seen at Ibadan, Nigeria.", "content": "The salient features of pulmonary tuberculosis in 263 Nigerian children are presented. Attention is drawn to the little known diagnostic presence of supraclavicular lymphadenopathy which is often accompanied by pulmonary tuberculosis. In the childhood population where early diagnosis of tuberculosis is sometimes difficult and depends mostly on a high index of suspicion, the finding of such a sign is highly significant and useful. Even though phlyctenular kerato-conjunctivitis has been reported to be caused more often by bacterial antigens other than tuberculosis, the experience in this part of the world is that its presence is still almost pathognomonic of tuberculosis. Outpatient ambulatory management though successful was associated with a high default rate. Suggestions are made on ways to reduce this rate.", "contents": "Pulmonary tuberculosis in childhood. An analysis of 263 cases seen at Ibadan, Nigeria. The salient features of pulmonary tuberculosis in 263 Nigerian children are presented. Attention is drawn to the little known diagnostic presence of supraclavicular lymphadenopathy which is often accompanied by pulmonary tuberculosis. In the childhood population where early diagnosis of tuberculosis is sometimes difficult and depends mostly on a high index of suspicion, the finding of such a sign is highly significant and useful. Even though phlyctenular kerato-conjunctivitis has been reported to be caused more often by bacterial antigens other than tuberculosis, the experience in this part of the world is that its presence is still almost pathognomonic of tuberculosis. Outpatient ambulatory management though successful was associated with a high default rate. Suggestions are made on ways to reduce this rate."}
{"id": "PMID:483371", "title": "Prednisolone in the treatment of pneumococcal meningitis.", "content": "The results of a steroid on 52 Nigerians (27 males, 25 females, aged 10 to 59 years) with bacteriologically proven pneumococcal meningitis are presented. Twenty-four of the patients were treated with steroids in addition to a standard regime. The clinical features on admission in the two groups were comparable and management other than steroid administration identical. Personality change with impairment of intellect was more frequent and mortality higher in the steroid-treated group although the differences wre not statistically significant. It is concluded that steroids apparently have no beneficial effect and may worsen the prognosis in pneumococcal meningitis.", "contents": "Prednisolone in the treatment of pneumococcal meningitis. The results of a steroid on 52 Nigerians (27 males, 25 females, aged 10 to 59 years) with bacteriologically proven pneumococcal meningitis are presented. Twenty-four of the patients were treated with steroids in addition to a standard regime. The clinical features on admission in the two groups were comparable and management other than steroid administration identical. Personality change with impairment of intellect was more frequent and mortality higher in the steroid-treated group although the differences wre not statistically significant. It is concluded that steroids apparently have no beneficial effect and may worsen the prognosis in pneumococcal meningitis."}
{"id": "PMID:483372", "title": "Haemorrhagic pleural effusion in Nigerians.", "content": "Experience with haemorrhagic pleural effusion in Nigerians is described. Sixty three cases selected from 283 cases of pleural effusion over a 15-year period were analysed. Haemorrhagic pleural effusion accounted for 22% of all cases of pleural effusion. Malignant cells were found in haemorrhagic fluid in 21% of cases. The commonest cause of haemorrhagic effusion was neoplasm followed by tuberculosis and trauma. Among neoplasms carcinoma of the bronchus was responsable for about half of such cases.", "contents": "Haemorrhagic pleural effusion in Nigerians. Experience with haemorrhagic pleural effusion in Nigerians is described. Sixty three cases selected from 283 cases of pleural effusion over a 15-year period were analysed. Haemorrhagic pleural effusion accounted for 22% of all cases of pleural effusion. Malignant cells were found in haemorrhagic fluid in 21% of cases. The commonest cause of haemorrhagic effusion was neoplasm followed by tuberculosis and trauma. Among neoplasms carcinoma of the bronchus was responsable for about half of such cases."}
{"id": "PMID:483373", "title": "Colorectal carcinoids in South India.", "content": "Twenty cases of colorectal carcinoids are reviewed in this report. Among the South Indian Asiatic, the colorectal carcinoids are apparently more common than appendicular and small intestinal carcinoids. The data obtained in this survey and the experience in America, citing increased incidence of such carcinoids in Negroes, suggest that racial factors are responsible for certain differences. The tendency to involve younger age groups and many cases having aggressive potentialities are other variations encountered in this review. A brief summary of its characteristic histopathology is given.", "contents": "Colorectal carcinoids in South India. Twenty cases of colorectal carcinoids are reviewed in this report. Among the South Indian Asiatic, the colorectal carcinoids are apparently more common than appendicular and small intestinal carcinoids. The data obtained in this survey and the experience in America, citing increased incidence of such carcinoids in Negroes, suggest that racial factors are responsible for certain differences. The tendency to involve younger age groups and many cases having aggressive potentialities are other variations encountered in this review. A brief summary of its characteristic histopathology is given."}
{"id": "PMID:483374", "title": "Intrauterine growth of live-born Tanzanian infants.", "content": "The incidence of low birthweight infants, the effect of intrauterine growth retardation on anthropometric measurements, and local standards of intrauterine growth curves for weight, crown-heel length and head circumference together with curves of body measurement ratios of weight/length, weight/head circumference and weight/length x head circumference are presented for the population of Dar es Salaam (Tanzania). Acomparison is made with a standard composed for a Caucasian community with completely different socio-economic and nutritional status. From 28 to 34 weeks of gestation weight gain in Dar es Salaam foetuses was reduced, while length and head circumference were relatively less affected. However, an increased intrauterine growth velocity was recorded from 34 to 38 weeks of gestation.", "contents": "Intrauterine growth of live-born Tanzanian infants. The incidence of low birthweight infants, the effect of intrauterine growth retardation on anthropometric measurements, and local standards of intrauterine growth curves for weight, crown-heel length and head circumference together with curves of body measurement ratios of weight/length, weight/head circumference and weight/length x head circumference are presented for the population of Dar es Salaam (Tanzania). Acomparison is made with a standard composed for a Caucasian community with completely different socio-economic and nutritional status. From 28 to 34 weeks of gestation weight gain in Dar es Salaam foetuses was reduced, while length and head circumference were relatively less affected. However, an increased intrauterine growth velocity was recorded from 34 to 38 weeks of gestation."}
{"id": "PMID:483375", "title": "Urolithiasis in Sudan. Geographical distribution and the influence of climate.", "content": "Urolithiasis is common throughout the Sudan with the exception of the southern region where it is rare. The latter region is cooler and decidedly more moist than the other provinces during the hot months of the year (May-October). In the northern provinces urolithiasis is commoner in areas with lower relative humidity.", "contents": "Urolithiasis in Sudan. Geographical distribution and the influence of climate. Urolithiasis is common throughout the Sudan with the exception of the southern region where it is rare. The latter region is cooler and decidedly more moist than the other provinces during the hot months of the year (May-October). In the northern provinces urolithiasis is commoner in areas with lower relative humidity."}
{"id": "PMID:483376", "title": "Leg ulceration in sickle cell disease in Nigeria.", "content": "Leg ulceration was found in only 14 of 834 (1.7%) sickle cell patients seen in a Lagos Hospital. Ulcers were seen only in patients above the age of 12 years in whom the prevalence was 5.4%. Affected males outnumbered females 6 to 1. The aetiology was traumatic in 12 patients, spontaneous in three and due to furuncle in one. The commonest sites were around the ankles. Ulceration was not commoner in patients with low socioeconomic status. The simplest forms of aseptic treatment resulted in as variable a healing time as more complex forms of treatment, but a high recurrence rate of ulceration (71.4%) was the major problem. There remains no acceptable reason for the lower prevalence of leg ulceration in Africans with sickle cell disease, but the role of zinc in susceptibility to the ulcers deserves further investigation.", "contents": "Leg ulceration in sickle cell disease in Nigeria. Leg ulceration was found in only 14 of 834 (1.7%) sickle cell patients seen in a Lagos Hospital. Ulcers were seen only in patients above the age of 12 years in whom the prevalence was 5.4%. Affected males outnumbered females 6 to 1. The aetiology was traumatic in 12 patients, spontaneous in three and due to furuncle in one. The commonest sites were around the ankles. Ulceration was not commoner in patients with low socioeconomic status. The simplest forms of aseptic treatment resulted in as variable a healing time as more complex forms of treatment, but a high recurrence rate of ulceration (71.4%) was the major problem. There remains no acceptable reason for the lower prevalence of leg ulceration in Africans with sickle cell disease, but the role of zinc in susceptibility to the ulcers deserves further investigation."}
{"id": "PMID:483377", "title": "The D-xylose absorption test in different ethnic groups.", "content": "Fifty-nine hospital in-patients in England, Saudi Arabia and Zambia had 5-h urinary xylose measured after a 25 g oral load; in 47 of them blood xylose concentration at 90 min. was measured. All of them came from an urban environment and none had significant organic disease. Exactly similar methods were used in each country; the investigator was the same in all of them. Mean xylose excretion was very similar in the English, Asians and Africans. The high incidence of abnormal xylose absorption tests in indigenous populations in tropical countries is due to environmental factors; it does not have a genetic basis.", "contents": "The D-xylose absorption test in different ethnic groups. Fifty-nine hospital in-patients in England, Saudi Arabia and Zambia had 5-h urinary xylose measured after a 25 g oral load; in 47 of them blood xylose concentration at 90 min. was measured. All of them came from an urban environment and none had significant organic disease. Exactly similar methods were used in each country; the investigator was the same in all of them. Mean xylose excretion was very similar in the English, Asians and Africans. The high incidence of abnormal xylose absorption tests in indigenous populations in tropical countries is due to environmental factors; it does not have a genetic basis."}
{"id": "PMID:483378", "title": "Plasma insulin and human growth hormone patterns in an African population.", "content": "Low fasting blood sugar levels and insulin sensitivity have been consistently reported in Africans. To study the role played by insulin and growth hormone (HGH) in the genesis of this state, plasma insulin and HGH levels were measured in 40 healthy fasting Africans. Mean plasma insulin and HGH were 10.7 +/- 1.3 muU/ml and less than 1 muU/ml respectively. These are in the same range reported for Caucasians in the literature. The response of plasma insulin and HGH to a menal was further studied in 80 healthy Africans. While insulin response was brisk and adequate, the HGH response was rather sluggish. The significance of these findings is discussed.", "contents": "Plasma insulin and human growth hormone patterns in an African population. Low fasting blood sugar levels and insulin sensitivity have been consistently reported in Africans. To study the role played by insulin and growth hormone (HGH) in the genesis of this state, plasma insulin and HGH levels were measured in 40 healthy fasting Africans. Mean plasma insulin and HGH were 10.7 +/- 1.3 muU/ml and less than 1 muU/ml respectively. These are in the same range reported for Caucasians in the literature. The response of plasma insulin and HGH to a menal was further studied in 80 healthy Africans. While insulin response was brisk and adequate, the HGH response was rather sluggish. The significance of these findings is discussed."}
{"id": "PMID:483379", "title": "Quantitative aspects of the development of mosquito transmitted Brugia malayi and Brugia pahangi and their distribution in Jirds, Meriones unguiculatus.", "content": "Twenty-two jirds, Meriones unguiculatus, were exposed to the bites of 2250 females of Aedes aegypti carrying an estimated total of 2464 larvae of Brugia malayi, and 13 jirds were offered for blood feeding to 1450 mosquitoes infected with about 4460 larvae of Brugia pahangi. On necropsy of the jirds, four months after feeding of the mosquitoes, a total of 88 adult filariae of B. malayi and 143 of B. pahangi were recovered in 20 and 13 jirds respectively. The majority of the adult filariae was obtained from the testes (48,9% of B. malayi and 53,2% of B. pahangi), from the heart-lung-system (26,1% and 45,6%), and additional in B. malayi from the tail (19,3%). It can be estimated that 3,6% (3,2%) of all infective larvae of B. malayi (B. pahangi), carried by Aedes aegypti females before feeding, reached maturity in the jird host after they had left the vectors during the blood meal. Microfilariae were found in the peripheral blood in seven of B. malayi infected jirds and in eleven of the jirds infected with B. pahangi.", "contents": "Quantitative aspects of the development of mosquito transmitted Brugia malayi and Brugia pahangi and their distribution in Jirds, Meriones unguiculatus. Twenty-two jirds, Meriones unguiculatus, were exposed to the bites of 2250 females of Aedes aegypti carrying an estimated total of 2464 larvae of Brugia malayi, and 13 jirds were offered for blood feeding to 1450 mosquitoes infected with about 4460 larvae of Brugia pahangi. On necropsy of the jirds, four months after feeding of the mosquitoes, a total of 88 adult filariae of B. malayi and 143 of B. pahangi were recovered in 20 and 13 jirds respectively. The majority of the adult filariae was obtained from the testes (48,9% of B. malayi and 53,2% of B. pahangi), from the heart-lung-system (26,1% and 45,6%), and additional in B. malayi from the tail (19,3%). It can be estimated that 3,6% (3,2%) of all infective larvae of B. malayi (B. pahangi), carried by Aedes aegypti females before feeding, reached maturity in the jird host after they had left the vectors during the blood meal. Microfilariae were found in the peripheral blood in seven of B. malayi infected jirds and in eleven of the jirds infected with B. pahangi."}
{"id": "PMID:483380", "title": "[A method to detect microfilariae of Dipetalonema witei in the peripheral blood of mastomys natalensis (author's transl)].", "content": "A new cheap and time saving procedure is described for the detection of microfilariae of Dipetalonema witei in the peripheral blood of Mastomys natalensis. A special apparatus delivering an ether-air mixture is used to anesthetize the experimental animals for a short period in a very careful way and without side effects. The ether wapour induces a rapid shift of microfilariae within one minute from the lung to the peripheral blood.", "contents": "[A method to detect microfilariae of Dipetalonema witei in the peripheral blood of mastomys natalensis (author's transl)]. A new cheap and time saving procedure is described for the detection of microfilariae of Dipetalonema witei in the peripheral blood of Mastomys natalensis. A special apparatus delivering an ether-air mixture is used to anesthetize the experimental animals for a short period in a very careful way and without side effects. The ether wapour induces a rapid shift of microfilariae within one minute from the lung to the peripheral blood."}
{"id": "PMID:483382", "title": "[Demonstration of Precipitating antibodies in patients with echinococcosis using a counter immunoelectrophoresis (author's transl)].", "content": "A counter immunoelectrophoresis was used for the demonstration of antibodies to echinococci. The antigen was prepared from hydatid fluid obtained from liver cysts of sheep. After concentration the antigenic material contained 1800 to 2900 micrograms of nitrogen per milliliter fluid. One or more precipitating lines were regarded as positive. Serum samples of 184 patients with surgically proven echinococcosis gave positive results in 80,4% of the cases. Among them, patients with hydatid disease of the liver were positive in 83,2% of the cases, and patients with hydatid disease of the lung were positive in 78,6 of the cases. The arithmetric mean of the number of lines was 2,4. Sera of 1182 patients with other diseases (among others diseases of the gastro-intestinal and respiratory tract, allergies, neuro-psychiatric, gynecologic and parasitic diseases) reached in 4,6% of the cases with the formation of precipitating lines. The described test is simple to perform, it can be read after a short period of time and it can be used as a screening test for mass case finding.", "contents": "[Demonstration of Precipitating antibodies in patients with echinococcosis using a counter immunoelectrophoresis (author's transl)]. A counter immunoelectrophoresis was used for the demonstration of antibodies to echinococci. The antigen was prepared from hydatid fluid obtained from liver cysts of sheep. After concentration the antigenic material contained 1800 to 2900 micrograms of nitrogen per milliliter fluid. One or more precipitating lines were regarded as positive. Serum samples of 184 patients with surgically proven echinococcosis gave positive results in 80,4% of the cases. Among them, patients with hydatid disease of the liver were positive in 83,2% of the cases, and patients with hydatid disease of the lung were positive in 78,6 of the cases. The arithmetric mean of the number of lines was 2,4. Sera of 1182 patients with other diseases (among others diseases of the gastro-intestinal and respiratory tract, allergies, neuro-psychiatric, gynecologic and parasitic diseases) reached in 4,6% of the cases with the formation of precipitating lines. The described test is simple to perform, it can be read after a short period of time and it can be used as a screening test for mass case finding."}
{"id": "PMID:483383", "title": "Trypanosome infections in domestic animals in Liberia.", "content": "In 5 domestic animal species of the Liberian rain forest, investigations were done on the infection rates with trypanosomes of the subgenera Nannomonas, Duttonella and Trypanozoon and on possible reservoir hosts for Trypanosoma (T.) brucei gambiense. Serological screening (indirect fluorescent antibody test) revealed that infection rates with Trypanosoma spp. were in N'Dama cattle 80.4%, in pigs 76.0%, in dogs 48.5%, in goats 35.1% and in sheep 28.1%. In N'Damas, antibodies to T. (N.) congolense and to T. (D.) vivax predominated and were distributed homogeneously; in pigs, antibodies to T. (N.) congolense were found most frequently. In dogs, antibodies to T. (N.) congolense and to T. (T.) b. brucei, and in goats and sheep antibodies to all 3 species were distributed homogeneously. Strong serological reactions (titres greater than 1:320) to T. (T.) b. brucei antigen were found most frequently in pigs (20.0%), in cattle (9.8%) and in dogs (9.5%). As suspicious reservoir hosts for T. (T.) b. gambiense, pigs (117) and dogs (105) were examined parasitologically in order to isolate Trypanozoon stocks for further infraspecific characterization. 56.4% of the pigs (14.5% Trypanozoon, 23.1% mixed Trypanozoon/Nannomonas, 18.8% Nannomonas), and 38.2% of the dogs (2.9% Trypanozoon, 14.3% mixed Trypanozoon/Nannomonas, 21.0% Nannomonas) were found to be infected. The sensitivities of the thick smear, the haematocrit centrifugation technique and the animal inoculation using Mastomys natalensis were described. 32 trypanosome stocks containing Trypanozoon were stabilized.", "contents": "Trypanosome infections in domestic animals in Liberia. In 5 domestic animal species of the Liberian rain forest, investigations were done on the infection rates with trypanosomes of the subgenera Nannomonas, Duttonella and Trypanozoon and on possible reservoir hosts for Trypanosoma (T.) brucei gambiense. Serological screening (indirect fluorescent antibody test) revealed that infection rates with Trypanosoma spp. were in N'Dama cattle 80.4%, in pigs 76.0%, in dogs 48.5%, in goats 35.1% and in sheep 28.1%. In N'Damas, antibodies to T. (N.) congolense and to T. (D.) vivax predominated and were distributed homogeneously; in pigs, antibodies to T. (N.) congolense were found most frequently. In dogs, antibodies to T. (N.) congolense and to T. (T.) b. brucei, and in goats and sheep antibodies to all 3 species were distributed homogeneously. Strong serological reactions (titres greater than 1:320) to T. (T.) b. brucei antigen were found most frequently in pigs (20.0%), in cattle (9.8%) and in dogs (9.5%). As suspicious reservoir hosts for T. (T.) b. gambiense, pigs (117) and dogs (105) were examined parasitologically in order to isolate Trypanozoon stocks for further infraspecific characterization. 56.4% of the pigs (14.5% Trypanozoon, 23.1% mixed Trypanozoon/Nannomonas, 18.8% Nannomonas), and 38.2% of the dogs (2.9% Trypanozoon, 14.3% mixed Trypanozoon/Nannomonas, 21.0% Nannomonas) were found to be infected. The sensitivities of the thick smear, the haematocrit centrifugation technique and the animal inoculation using Mastomys natalensis were described. 32 trypanosome stocks containing Trypanozoon were stabilized."}
{"id": "PMID:483384", "title": "Ecological studies at Hofuf, Eastern Saudi Arabia, in relation to dermal leishmaniasis.", "content": "Field and laboratory investigations were carried out in connection with an epidemic of dermal leishmaniasis in Hofuf, Eastern Province of Saudi Arabia, during 1977. In this present note emphasis is laid on description of the population dynamics and species spectrum of the sandflies, the occurrence of the vector and the course of the epidemic of Leishmania tropica in correlation with the sandfly population and the natural reservoir and the rodent population.", "contents": "Ecological studies at Hofuf, Eastern Saudi Arabia, in relation to dermal leishmaniasis. Field and laboratory investigations were carried out in connection with an epidemic of dermal leishmaniasis in Hofuf, Eastern Province of Saudi Arabia, during 1977. In this present note emphasis is laid on description of the population dynamics and species spectrum of the sandflies, the occurrence of the vector and the course of the epidemic of Leishmania tropica in correlation with the sandfly population and the natural reservoir and the rodent population."}
{"id": "PMID:483385", "title": "Inhibition of bovine bone marrow granulocyte/macrophage colony formation in vitro by serum collected from cattle infected with Trypanosoma vivax or Trypanosoma congolense.", "content": "Serum collected from cattle infected with Trypanosoma vivax or Trypanosoma congolense inhibited bovine granulocyte/macrophage colony formation in methyl cellulose cultures. Maximum inhibition was caused by serum collected two and three weeks post infection. The degree of inhibition appeared to be related to the degree of parasitemia. Inhibition of erythroid colony formation by serum collected from these animals was not observed.", "contents": "Inhibition of bovine bone marrow granulocyte/macrophage colony formation in vitro by serum collected from cattle infected with Trypanosoma vivax or Trypanosoma congolense. Serum collected from cattle infected with Trypanosoma vivax or Trypanosoma congolense inhibited bovine granulocyte/macrophage colony formation in methyl cellulose cultures. Maximum inhibition was caused by serum collected two and three weeks post infection. The degree of inhibition appeared to be related to the degree of parasitemia. Inhibition of erythroid colony formation by serum collected from these animals was not observed."}
{"id": "PMID:483386", "title": "Evaluation of a new long-acting oxytetracycline formulation against anaplasmosis in colombian cattle.", "content": "A long-acting injectable (Terramycin/LA) forulated to contain 200 mg/ml of oxytetracycline was tested and compared to a commercially available oxytetracycline injectable (Emicina) containing 50 mg/ml in the treatments of experimentally induced Anaplasma marginale infection in Colombian cattle. Group 1, consisting of 10 infected calves, served as non-treated controls. Ten infected calves (Group 2) were treated with two doses of Emicina, each at 10 mg/kg, intramuscularly (IM), and another ten infected calves (Group 3) were treated once IM, with 20 mg/kg of Terramycin/LA. All treatments were given during the acute phase of infection after A. marginale parasitemia was demonstrated and when the packed cell volume (PCV) had decreased to 60-64% of normal value. Two treatments with Emicina and one with Terramycin/LA were both effective in moderating the course of A. marginale infection and in preventing serious clinical signs of disease under laboratory conditions.", "contents": "Evaluation of a new long-acting oxytetracycline formulation against anaplasmosis in colombian cattle. A long-acting injectable (Terramycin/LA) forulated to contain 200 mg/ml of oxytetracycline was tested and compared to a commercially available oxytetracycline injectable (Emicina) containing 50 mg/ml in the treatments of experimentally induced Anaplasma marginale infection in Colombian cattle. Group 1, consisting of 10 infected calves, served as non-treated controls. Ten infected calves (Group 2) were treated with two doses of Emicina, each at 10 mg/kg, intramuscularly (IM), and another ten infected calves (Group 3) were treated once IM, with 20 mg/kg of Terramycin/LA. All treatments were given during the acute phase of infection after A. marginale parasitemia was demonstrated and when the packed cell volume (PCV) had decreased to 60-64% of normal value. Two treatments with Emicina and one with Terramycin/LA were both effective in moderating the course of A. marginale infection and in preventing serious clinical signs of disease under laboratory conditions."}
{"id": "PMID:483387", "title": "Pancarditis in Trypanosoma vivax infection in cattle.", "content": "In cattle chronically infected with T. vivax Y 58, a mononuclear pancarditis with extravascular trypanosomes is described. It has been considered as a local immune response to these extravascular trypanosomes. No autoantibodies for heart tissue have been found.", "contents": "Pancarditis in Trypanosoma vivax infection in cattle. In cattle chronically infected with T. vivax Y 58, a mononuclear pancarditis with extravascular trypanosomes is described. It has been considered as a local immune response to these extravascular trypanosomes. No autoantibodies for heart tissue have been found."}
{"id": "PMID:483388", "title": "[A new lung fluke from the coastal range of ecuador: Paragonimus ecuadoriensis n.sp. (paragonimidae, trematoda) (author's transl)].", "content": "P. ecuadoriensis in the first lung fluke described from Ecuador. The description is based on 10 adult flukes from a natural infected Coati (Nasua nasua), 23 adults from 2 experimentally infected cats, and 200 metacercariae from the second intermediate host, the freshwater crab Hypolobocera aequatorialis. The new species is characterized by the following features: mouth succer larger than acetabulum or nearly of the same size; integument spines (\"cuticular\" spines) single; ovary with 5-6 short branches, which divide into more or less short broad lobes; testes larger than ovary with 5-6 simple or polymorphic lobes; eggs longish-ovoid, shell thin and uniform in thickness; surface undulated. Metacercariae not encysted. The adults of the new species are differentiated from probably related lung flukes P. peruvianus, P. mexicanus, and P. inca by the different morphology of ovary and testes, and by the size and form of the eggs. The metacercariae of P. ecuadoriensis are located not only in the hepatopancreas of the crab host - as it is in the case of P. peruvianus - but also and more frequently in the musculature. Paragonimus-eggs from sputum of two patients show partly the same characteristics as the eggs from animal hosts. One may therefore assume that P. ecuadoriensis is infecting humans in the investigated area.", "contents": "[A new lung fluke from the coastal range of ecuador: Paragonimus ecuadoriensis n.sp. (paragonimidae, trematoda) (author's transl)]. P. ecuadoriensis in the first lung fluke described from Ecuador. The description is based on 10 adult flukes from a natural infected Coati (Nasua nasua), 23 adults from 2 experimentally infected cats, and 200 metacercariae from the second intermediate host, the freshwater crab Hypolobocera aequatorialis. The new species is characterized by the following features: mouth succer larger than acetabulum or nearly of the same size; integument spines (\"cuticular\" spines) single; ovary with 5-6 short branches, which divide into more or less short broad lobes; testes larger than ovary with 5-6 simple or polymorphic lobes; eggs longish-ovoid, shell thin and uniform in thickness; surface undulated. Metacercariae not encysted. The adults of the new species are differentiated from probably related lung flukes P. peruvianus, P. mexicanus, and P. inca by the different morphology of ovary and testes, and by the size and form of the eggs. The metacercariae of P. ecuadoriensis are located not only in the hepatopancreas of the crab host - as it is in the case of P. peruvianus - but also and more frequently in the musculature. Paragonimus-eggs from sputum of two patients show partly the same characteristics as the eggs from animal hosts. One may therefore assume that P. ecuadoriensis is infecting humans in the investigated area."}
{"id": "PMID:483392", "title": "[Action of x-ray irradiation on the nuclear envelope of rat liver cells].", "content": "X-irradiation of isolated nuclear envelopes (NE) has revealed their high radiosensitivity, while irradiation of isolated intact nuclei in vitro, in the doses up to 5000 r 18--20 hours after partial hepatectomy, produced no morphological changes in NE. The damaging effect of irradiation on both nuclei and mitochondria (Mt) was revealed only with a decrease in cytochrome-c-oxidase (CO) activity in parallel with an increase in the radiation dose. One hour after the whole body irradiation of rats in the beginning of S-period, the damaging effect was recorded in both NE and Mt at the doses of 50 and 150 t, and was enhanced with the increase of irradiation dose. Morphological changes were observed mostly in the outer nuclear membrane, which lost its distinct outline and disappeared from some nuclear regions. Lethal radiation doses produced a decrease in the number of pore complexes (PC) with their evident segregation from the membranes. After irradiation in a dose of 1200 r, only the residue or \"ghosts\" of the PCs remained. After irradiation in doses up to 400 r, the CO-activity recovered during the first hour in Mt and during first two hours in the nuclei.", "contents": "[Action of x-ray irradiation on the nuclear envelope of rat liver cells]. X-irradiation of isolated nuclear envelopes (NE) has revealed their high radiosensitivity, while irradiation of isolated intact nuclei in vitro, in the doses up to 5000 r 18--20 hours after partial hepatectomy, produced no morphological changes in NE. The damaging effect of irradiation on both nuclei and mitochondria (Mt) was revealed only with a decrease in cytochrome-c-oxidase (CO) activity in parallel with an increase in the radiation dose. One hour after the whole body irradiation of rats in the beginning of S-period, the damaging effect was recorded in both NE and Mt at the doses of 50 and 150 t, and was enhanced with the increase of irradiation dose. Morphological changes were observed mostly in the outer nuclear membrane, which lost its distinct outline and disappeared from some nuclear regions. Lethal radiation doses produced a decrease in the number of pore complexes (PC) with their evident segregation from the membranes. After irradiation in a dose of 1200 r, only the residue or \"ghosts\" of the PCs remained. After irradiation in doses up to 400 r, the CO-activity recovered during the first hour in Mt and during first two hours in the nuclei."}
{"id": "PMID:483393", "title": "[Change in the ultrastructure of hepatocyte contacts in mechanical deformations of liver tissue].", "content": "Rat liver specimens were deformed by compression between two parallel plates up to the state of plastic deformation to study with the electron microscope. The mechanical deformation was followed by a destruction of intercellular contacts and separation of hepatocytes. According to the pattern of destruction, all the contact elements can be divided into two groups. 1. Simple junction, desmosome, intermediate junction which exibit a wide range of structural changes prior to the moment of destruction. In this case, the process of cell separation proceeds in the adhesive way. 2. Tight and gap junctions which exibit a higher structural stability. In this case the process of cell separation appears to be of the cohesine nature.", "contents": "[Change in the ultrastructure of hepatocyte contacts in mechanical deformations of liver tissue]. Rat liver specimens were deformed by compression between two parallel plates up to the state of plastic deformation to study with the electron microscope. The mechanical deformation was followed by a destruction of intercellular contacts and separation of hepatocytes. According to the pattern of destruction, all the contact elements can be divided into two groups. 1. Simple junction, desmosome, intermediate junction which exibit a wide range of structural changes prior to the moment of destruction. In this case, the process of cell separation proceeds in the adhesive way. 2. Tight and gap junctions which exibit a higher structural stability. In this case the process of cell separation appears to be of the cohesine nature."}
{"id": "PMID:483394", "title": "[Changes in hepatocyte ultrastructure under the influence of chronic furacillin administration to rats].", "content": "The ultrastructure of rat liver cells was studied during a 6 months' furacilline (5-nitro-2-furtfurilsemicarbazon) feeding in dose of 40 mg per day, and also during the next 8 months after the treatment cessation. An irregular swelling of membranous structure in addition to disorganization and partial reduction of the granular endoplasmic reticulum were found in the hepatocyte cytoplasm after the prolonged furacilline feeding as well as glycogen depletion and the tendency of the agranular endoplasmic reticulum to enlargement. These changes rarely reached the essential intensity with the transition to necrobiosis. They disappeared already a month after the cessation of furacilline treatment. In all the terms of experiment, the nucleoli were hypertrophied and retained their loose nucleoloneme structure. No sings of furacilline carcinogenic activity were found in rat liver during the 14 months of investigation.", "contents": "[Changes in hepatocyte ultrastructure under the influence of chronic furacillin administration to rats]. The ultrastructure of rat liver cells was studied during a 6 months' furacilline (5-nitro-2-furtfurilsemicarbazon) feeding in dose of 40 mg per day, and also during the next 8 months after the treatment cessation. An irregular swelling of membranous structure in addition to disorganization and partial reduction of the granular endoplasmic reticulum were found in the hepatocyte cytoplasm after the prolonged furacilline feeding as well as glycogen depletion and the tendency of the agranular endoplasmic reticulum to enlargement. These changes rarely reached the essential intensity with the transition to necrobiosis. They disappeared already a month after the cessation of furacilline treatment. In all the terms of experiment, the nucleoli were hypertrophied and retained their loose nucleoloneme structure. No sings of furacilline carcinogenic activity were found in rat liver during the 14 months of investigation."}
{"id": "PMID:483389", "title": "[Sex ratio and survival rate in embryogenesis and the perinatal period].", "content": "Sex ratio was studied among abortuses and infants who died perinatally (9972 cases). Sex begins influencing mortality in the perinatal period, in which a significant increase in the sex ratio is shown (106 males: 100 females--antenatal; 136 males: 100 females--intranatal; 159 males:100 females--within the first 6 days of life).", "contents": "[Sex ratio and survival rate in embryogenesis and the perinatal period]. Sex ratio was studied among abortuses and infants who died perinatally (9972 cases). Sex begins influencing mortality in the perinatal period, in which a significant increase in the sex ratio is shown (106 males: 100 females--antenatal; 136 males: 100 females--intranatal; 159 males:100 females--within the first 6 days of life)."}
{"id": "PMID:483395", "title": "[Cells with unusual mitochondria in the thyroid gland of an old rat].", "content": "Thyroid glands of 3 year old rats have been studied by electron microscopy. The cells with changed mitochondria were found next to the typical thyrocytes in the follicle walls. The large mitochondria with dense matrix and tubular cristae often occupy the major part of the cytoplasm in these cells. On this basis, the author considers the changed cells to be the Askanazy cells, or oncocytes. Mitochondria-rich cells were found also among calcitocytes. The origin of oncocytes from usual thyrocytes and calcitocytes is under discussion.", "contents": "[Cells with unusual mitochondria in the thyroid gland of an old rat]. Thyroid glands of 3 year old rats have been studied by electron microscopy. The cells with changed mitochondria were found next to the typical thyrocytes in the follicle walls. The large mitochondria with dense matrix and tubular cristae often occupy the major part of the cytoplasm in these cells. On this basis, the author considers the changed cells to be the Askanazy cells, or oncocytes. Mitochondria-rich cells were found also among calcitocytes. The origin of oncocytes from usual thyrocytes and calcitocytes is under discussion."}
{"id": "PMID:483390", "title": "[Effect of heredity on cattle morbidity from brucellosis].", "content": "A high constancy of diversity was found in the genealogical groups in respect to the brucellosis incidences. It is caused by the influence of sires. The frequency of brucellosis is significantly dependent on the year of birth, paternity and the interaction of the father's genotype with the year of birth of the daughters.", "contents": "[Effect of heredity on cattle morbidity from brucellosis]. A high constancy of diversity was found in the genealogical groups in respect to the brucellosis incidences. It is caused by the influence of sires. The frequency of brucellosis is significantly dependent on the year of birth, paternity and the interaction of the father's genotype with the year of birth of the daughters."}
{"id": "PMID:483396", "title": "[Variability of Chinese hamster clones in resistance to 8-azaguanine].", "content": "The stability of the 8-azaguanine resistance marker during the number of cell generations has been analyzed for 63 clones of Chinese hamster cells. The resistant clones analyzed were spontaneous or induced after the treatment of parent cells by 5-BUdR, EMS, or histone H1. The clones have revealed different levels of AG-resistance as well as different ability to maintain the above level, under the cultivation in the drug-free medium. Subclones, isolated from the unstable clone, were found to be different in both the resistance level and the stability of resistant state.", "contents": "[Variability of Chinese hamster clones in resistance to 8-azaguanine]. The stability of the 8-azaguanine resistance marker during the number of cell generations has been analyzed for 63 clones of Chinese hamster cells. The resistant clones analyzed were spontaneous or induced after the treatment of parent cells by 5-BUdR, EMS, or histone H1. The clones have revealed different levels of AG-resistance as well as different ability to maintain the above level, under the cultivation in the drug-free medium. Subclones, isolated from the unstable clone, were found to be different in both the resistance level and the stability of resistant state."}
{"id": "PMID:483397", "title": "[Electrophoretic study of the protein makepup of the muscles in Zenker's necrosis].", "content": "Changes of protein composition within necrotic areas of muscle at the late stages of Zenker's necrosis (3--5 hours after damage) have been studied using the disc-DSN-electrophoresis method. These changes are presumably associated with a disarrangement of the structure of thick and thin fillaments. The disturbance of the contractile system is accompanied by the loss of water soluble protein specific fractions.", "contents": "[Electrophoretic study of the protein makepup of the muscles in Zenker's necrosis]. Changes of protein composition within necrotic areas of muscle at the late stages of Zenker's necrosis (3--5 hours after damage) have been studied using the disc-DSN-electrophoresis method. These changes are presumably associated with a disarrangement of the structure of thick and thin fillaments. The disturbance of the contractile system is accompanied by the loss of water soluble protein specific fractions."}
{"id": "PMID:483391", "title": "[Results of a cytogenetic examination of population groups with intensive and limited use of pesticides].", "content": "Cytogenetic examination was carried out in two representative groups of teenagers living in zones with intense and limited use of pesticides. The average frequency of chromosome aberrations in the experimental zone was 1.35%, which exceeded significantly that in the control group. The found cytogenetic effect shows a potential genetic hazard of intense use of pesticides for the population.", "contents": "[Results of a cytogenetic examination of population groups with intensive and limited use of pesticides]. Cytogenetic examination was carried out in two representative groups of teenagers living in zones with intense and limited use of pesticides. The average frequency of chromosome aberrations in the experimental zone was 1.35%, which exceeded significantly that in the control group. The found cytogenetic effect shows a potential genetic hazard of intense use of pesticides for the population."}
{"id": "PMID:483490", "title": "Regional lymph flow in unanesthetized rabbits.", "content": "Determinations were made of the 2-hour 22Na space and the 1 and 2 hour 51Cr EDTA spaces in a number of tissues. In regions where the two 51Cr-EDTA spaces were similar and smaller than or similar to the 22Na space the former spaces were regarded as measures of the extra-cellular volume. Using previously published data for plasma volumes, extravascular plasma-equivalent albumin and IgG spaces and the turnover rate constants for the extravascular proteins, it was possible to calculate extravascular protein concentrations and rates of net filtration from the capillaries or lymph flow in some tissues. The albumin concentrations, probably underestimated, were about 40% of the plasma concentrations in small intestine, lung and skeletal muscle, higher in heart muscle and lower in stomach wall. The net filtration or lymph flow, probably overestimated, was 0.81, 1.5, 0.53, 0.61 and 0.12 microliters/min per g in heart muscle, small intestine, lung, stomach wall and skeletal muscle, respectively.", "contents": "Regional lymph flow in unanesthetized rabbits. Determinations were made of the 2-hour 22Na space and the 1 and 2 hour 51Cr EDTA spaces in a number of tissues. In regions where the two 51Cr-EDTA spaces were similar and smaller than or similar to the 22Na space the former spaces were regarded as measures of the extra-cellular volume. Using previously published data for plasma volumes, extravascular plasma-equivalent albumin and IgG spaces and the turnover rate constants for the extravascular proteins, it was possible to calculate extravascular protein concentrations and rates of net filtration from the capillaries or lymph flow in some tissues. The albumin concentrations, probably underestimated, were about 40% of the plasma concentrations in small intestine, lung and skeletal muscle, higher in heart muscle and lower in stomach wall. The net filtration or lymph flow, probably overestimated, was 0.81, 1.5, 0.53, 0.61 and 0.12 microliters/min per g in heart muscle, small intestine, lung, stomach wall and skeletal muscle, respectively."}
{"id": "PMID:483491", "title": "On how macromolecules reduce hemoglobin loss in hypotonic hemolysis.", "content": "When human red cells are hemolysed in hypotonic solutions containing macromolecules, the hemoglobin loss from the individual cells is reduced although the number of cells hemolysed is not affected. The evidence strongly suggests this is a colloid osmotic effect but an additional condition is also necessary if hemoglobin is to be retained. The cell must reseal, at least to hemoglobin and macromolecules. There is some evidence which points to the role of the macromolecule in this process. Further, at least in the case of dextrans, a minimal size of about 2000 daltons is required for suppression of hemoglobin liberation and it is suggested that this limit may be set by the diffusion coefficient.", "contents": "On how macromolecules reduce hemoglobin loss in hypotonic hemolysis. When human red cells are hemolysed in hypotonic solutions containing macromolecules, the hemoglobin loss from the individual cells is reduced although the number of cells hemolysed is not affected. The evidence strongly suggests this is a colloid osmotic effect but an additional condition is also necessary if hemoglobin is to be retained. The cell must reseal, at least to hemoglobin and macromolecules. There is some evidence which points to the role of the macromolecule in this process. Further, at least in the case of dextrans, a minimal size of about 2000 daltons is required for suppression of hemoglobin liberation and it is suggested that this limit may be set by the diffusion coefficient."}
{"id": "PMID:483493", "title": "Effects of various environmental temperatures on effort angina.", "content": "Eleven patients with effort angina and a history of cold intolerance performed short-term bicycle exercise tests at various room temperatures, 20, 10, 0 and -10 degrees C, and a few patients also at -30 degrees C. A significant reduction of maximal working capability (expressed as maximal work load, MWL), limited by moderately severe angina, was found at -10 degrees C (7% +/- 1, SEM, P less than 0.05) compared with normal room temperature. At 0 and 10 degrees C changes of MWL were small and not significant, and at -30 degrees C no further decrease of MWL was seen. About half of the patients, however, showed a tendency toward a decrease in MWL with decreasing environmental temperature, and and the decrease in MWL correlated significantly with an increase in rate pressure product (RPP) during exercise at both 0 and -10 degrees C. Thus, the decrease in working capability on exposure to cold could be explained by an increase in heart work. Warming up effects of exercise, counteracting the cold-induced increase in peripheral vascular resistance, were indicated by a diminishing difference in systolic blood pressure between a cold and normal environment with increasing work time.", "contents": "Effects of various environmental temperatures on effort angina. Eleven patients with effort angina and a history of cold intolerance performed short-term bicycle exercise tests at various room temperatures, 20, 10, 0 and -10 degrees C, and a few patients also at -30 degrees C. A significant reduction of maximal working capability (expressed as maximal work load, MWL), limited by moderately severe angina, was found at -10 degrees C (7% +/- 1, SEM, P less than 0.05) compared with normal room temperature. At 0 and 10 degrees C changes of MWL were small and not significant, and at -30 degrees C no further decrease of MWL was seen. About half of the patients, however, showed a tendency toward a decrease in MWL with decreasing environmental temperature, and and the decrease in MWL correlated significantly with an increase in rate pressure product (RPP) during exercise at both 0 and -10 degrees C. Thus, the decrease in working capability on exposure to cold could be explained by an increase in heart work. Warming up effects of exercise, counteracting the cold-induced increase in peripheral vascular resistance, were indicated by a diminishing difference in systolic blood pressure between a cold and normal environment with increasing work time."}
{"id": "PMID:483494", "title": "Reaction to cold of patients with coronary insufficiency.", "content": "Twenty six patients with angina pectoris and coronary insufficiency as judged by an exercise ECG test were examined. About half of the patients had more pronounced ECG changes in a cold room at -15 degrees C than at room temperature. They worked less, their subjective rating of exertion during exercise was higher and the heart performed less work, expressed as the heart rate blood pressure product. The other half of the patients was not much influenced by cold. During an exercise test in the supine position almost all patients got more pronounced ECG changes, worked less and the heart performed less work than in the sitting position. It is suggested that cold exposure as well as a supine body position may to a considerable part exert their effect, i.e. lower the anginal threshold and increase ECG changes, by increasing the central blood volume and the diastolic volume of the left heart and thus ceteres paribus the myocardial oxygen consumption.", "contents": "Reaction to cold of patients with coronary insufficiency. Twenty six patients with angina pectoris and coronary insufficiency as judged by an exercise ECG test were examined. About half of the patients had more pronounced ECG changes in a cold room at -15 degrees C than at room temperature. They worked less, their subjective rating of exertion during exercise was higher and the heart performed less work, expressed as the heart rate blood pressure product. The other half of the patients was not much influenced by cold. During an exercise test in the supine position almost all patients got more pronounced ECG changes, worked less and the heart performed less work than in the sitting position. It is suggested that cold exposure as well as a supine body position may to a considerable part exert their effect, i.e. lower the anginal threshold and increase ECG changes, by increasing the central blood volume and the diastolic volume of the left heart and thus ceteres paribus the myocardial oxygen consumption."}
{"id": "PMID:483495", "title": "Method for measurement of maximal isometric muscle strength with special reference to the fingers.", "content": "A method for measurement of the maximal isometric muscle strength in the fingers is presented. Details of the apparatus designed for avoidance of hysteresis in mechanical components on measurement of low strengths are given. The measurement of low strengths is described. The measurement procedure is simple both for examiner and for the subject. Values for finger muscle strength in normal persons are presented.", "contents": "Method for measurement of maximal isometric muscle strength with special reference to the fingers. A method for measurement of the maximal isometric muscle strength in the fingers is presented. Details of the apparatus designed for avoidance of hysteresis in mechanical components on measurement of low strengths are given. The measurement of low strengths is described. The measurement procedure is simple both for examiner and for the subject. Values for finger muscle strength in normal persons are presented."}
{"id": "PMID:483496", "title": "Complications of pelvic lymphadenectomy and retropubic prostatic 125I implantation.", "content": "Pelvic lymphadenectomy was accomplished in 75 patients with localized prostatic cancer at the time of retropubic prostatic implantation with iodine-125. Twelve (16 per cent) surgical complications were encountered in 11 patients and included 1 death. Late complications which persisted six months or longer occurred in 5 (8 per cent) of 61 patients. The most serious and as yet unquantitated complication appears to be pelvic vein thrombosis and pulmonary emboli. PELVIC LYMPHADenectomy may be accomplished safely and with minimal morbidity in the vast majority of prostatic cancer patients.", "contents": "Complications of pelvic lymphadenectomy and retropubic prostatic 125I implantation. Pelvic lymphadenectomy was accomplished in 75 patients with localized prostatic cancer at the time of retropubic prostatic implantation with iodine-125. Twelve (16 per cent) surgical complications were encountered in 11 patients and included 1 death. Late complications which persisted six months or longer occurred in 5 (8 per cent) of 61 patients. The most serious and as yet unquantitated complication appears to be pelvic vein thrombosis and pulmonary emboli. PELVIC LYMPHADenectomy may be accomplished safely and with minimal morbidity in the vast majority of prostatic cancer patients."}
{"id": "PMID:483497", "title": "Critical appraisal of routine supraclavicular lymph node biopsy in staging of testicular tumors.", "content": "Supraclavicular lymph node biopsy was performed as a staging procedure in 36 patients with germ cell tumors of the testis and nonpalpable supraclavicular nodes. Of 28 patients with clinical Stage A or B disease, 1 patient (4 per cent) was found to have supraclavicular metastases. Of 8 patients with clinical Stage C disease, 2 (25 per cent) had supraclavicular metastases. The apparent infrequency with which subclinical supradiaphragmatic disease is documented with this procedure and the current use of adjuvant systemic therapy in patients with pathologic Stage B nonseminomatous tumors suggest that supraclavicular lymph node biopsy should be abandoned as a routine staging procedure.", "contents": "Critical appraisal of routine supraclavicular lymph node biopsy in staging of testicular tumors. Supraclavicular lymph node biopsy was performed as a staging procedure in 36 patients with germ cell tumors of the testis and nonpalpable supraclavicular nodes. Of 28 patients with clinical Stage A or B disease, 1 patient (4 per cent) was found to have supraclavicular metastases. Of 8 patients with clinical Stage C disease, 2 (25 per cent) had supraclavicular metastases. The apparent infrequency with which subclinical supradiaphragmatic disease is documented with this procedure and the current use of adjuvant systemic therapy in patients with pathologic Stage B nonseminomatous tumors suggest that supraclavicular lymph node biopsy should be abandoned as a routine staging procedure."}
{"id": "PMID:483498", "title": "Transurethral electroincision of bladder neck in female patients with neurogenic bladder.", "content": "Transurethral electroincision of the bladder neck in female patients with neurogenic bladders has not been widely reported. We have performed this operation on 21 patients who have failed to achieve balanced bladder function through other treatment modalities and who have presented with recurrent urinary tract infections, high postvoid residual urine, and evidence of upper urinary tract deterioration. Eighty-five per cent of female patients treated in this fashion have demonstrated significant improvement in bladder emptying. The rate of complication has been low, and no cases of persistent incontinence have occurred. We recommend this operation in difficult cases of neurogenic bladder in females.", "contents": "Transurethral electroincision of bladder neck in female patients with neurogenic bladder. Transurethral electroincision of the bladder neck in female patients with neurogenic bladders has not been widely reported. We have performed this operation on 21 patients who have failed to achieve balanced bladder function through other treatment modalities and who have presented with recurrent urinary tract infections, high postvoid residual urine, and evidence of upper urinary tract deterioration. Eighty-five per cent of female patients treated in this fashion have demonstrated significant improvement in bladder emptying. The rate of complication has been low, and no cases of persistent incontinence have occurred. We recommend this operation in difficult cases of neurogenic bladder in females."}
{"id": "PMID:483499", "title": "Critical appraisal of oral calcium load test for indirect assessment of intestinal calcium absorption.", "content": "Two methods of oral calcium load or tolerance test for the indirect assessment of calcium absorption were compared. In 16 patients in whom the diagnosis of absorptive hypercalciuria was made independently, an exaggerated urinary total calcium excretion during four hours following calcium load, indicative of increased calcium absorption according to the method of Pak et al., was found in 15 patients. An abnormally high increment in urinary calcium during third and fourth hours post-calcium load, suggestive of enhanced calcium absorption by the criteria of Broadus et al., was encountered in 14 patients. However, an exaggerated urinary total calcium following calcium load was found in all 7 patients with renal hypercalciuria, whereas only 4 were shown to have an enhanced increment in calcium excretion. It is concluded that both methods are equally reliable in the detection of increased calcium absorption in absorptive hypercalciuria. However, the technique of Broadus et al. is probably superior to that of Pak et al. in the disclosure of increased calcium absorption in renal hypercalciuria.", "contents": "Critical appraisal of oral calcium load test for indirect assessment of intestinal calcium absorption. Two methods of oral calcium load or tolerance test for the indirect assessment of calcium absorption were compared. In 16 patients in whom the diagnosis of absorptive hypercalciuria was made independently, an exaggerated urinary total calcium excretion during four hours following calcium load, indicative of increased calcium absorption according to the method of Pak et al., was found in 15 patients. An abnormally high increment in urinary calcium during third and fourth hours post-calcium load, suggestive of enhanced calcium absorption by the criteria of Broadus et al., was encountered in 14 patients. However, an exaggerated urinary total calcium following calcium load was found in all 7 patients with renal hypercalciuria, whereas only 4 were shown to have an enhanced increment in calcium excretion. It is concluded that both methods are equally reliable in the detection of increased calcium absorption in absorptive hypercalciuria. However, the technique of Broadus et al. is probably superior to that of Pak et al. in the disclosure of increased calcium absorption in renal hypercalciuria."}
{"id": "PMID:483501", "title": "Home monitoring of penile tumescence for erectile dysfunction. Initial experience.", "content": "A technique using nocturnal penile tumescence monitoring has been developed to gather objective data on an outpatient basis. Patients have readily accepted the procedure and have easily learned to operate the monitor. Data from this group of controls and subjects are consistent with previously published reports by others. Suggestions are made from possible further refinements in technique.", "contents": "Home monitoring of penile tumescence for erectile dysfunction. Initial experience. A technique using nocturnal penile tumescence monitoring has been developed to gather objective data on an outpatient basis. Patients have readily accepted the procedure and have easily learned to operate the monitor. Data from this group of controls and subjects are consistent with previously published reports by others. Suggestions are made from possible further refinements in technique."}
{"id": "PMID:483500", "title": "Paradoxical increase of renal blood flow in anesthetized hypertensive dog treated with indomethacin.", "content": "To evaluate the effect of prostaglandin inhibition on the renal blood flow of the ischemic kidney, we administered indomethacin to 10 anesthetized dogs with renal artery stenosis and contralateral nephrectomy. Following the operation to produce renal ischemia, there was an increase of blood pressure associated with an increase of renin and the prostaglandins F1 (PGF1), and E (PGE). The administration of indomethacin to the intact, normotensive animals caused the anticipated decrease of prostaglandin E, renin, and renal blood flow. However, in the hypertensive dogs, indomethacin caused a paradoxical 45 per cent increase in the renal blood flow, despite a 44 per cent decrease of prostaglandin E. PGF1, PGE, renin, and erythropoietin exhibited the anticipated decreased levels. The study suggests that prostaglandins may not be the sole important factor in the regulation of renal blood flow in the presence of ischemia. Other important factors likely include the renin-sensitive angiotensin, the adrenergic, and the kallikrein-kinin systems.", "contents": "Paradoxical increase of renal blood flow in anesthetized hypertensive dog treated with indomethacin. To evaluate the effect of prostaglandin inhibition on the renal blood flow of the ischemic kidney, we administered indomethacin to 10 anesthetized dogs with renal artery stenosis and contralateral nephrectomy. Following the operation to produce renal ischemia, there was an increase of blood pressure associated with an increase of renin and the prostaglandins F1 (PGF1), and E (PGE). The administration of indomethacin to the intact, normotensive animals caused the anticipated decrease of prostaglandin E, renin, and renal blood flow. However, in the hypertensive dogs, indomethacin caused a paradoxical 45 per cent increase in the renal blood flow, despite a 44 per cent decrease of prostaglandin E. PGF1, PGE, renin, and erythropoietin exhibited the anticipated decreased levels. The study suggests that prostaglandins may not be the sole important factor in the regulation of renal blood flow in the presence of ischemia. Other important factors likely include the renin-sensitive angiotensin, the adrenergic, and the kallikrein-kinin systems."}
{"id": "PMID:483503", "title": "Adenocarcinoma of rete testis.", "content": "Adenocarcinoma of the rete testis is a rare tumor of the genital tract. An advanced case of adenocarcinoma thought to arise from the rete testis is presented. We believe this is the eighteenth reported case.", "contents": "Adenocarcinoma of rete testis. Adenocarcinoma of the rete testis is a rare tumor of the genital tract. An advanced case of adenocarcinoma thought to arise from the rete testis is presented. We believe this is the eighteenth reported case."}
{"id": "PMID:483504", "title": "Ureteral obstruction after aorto-femoral bypass surgery.", "content": "A case of ureteral obstruction from bypass surgery is presented with review of the literature. A pathogenesis for the obstruction has been suggested and specific treatment recommended. More importantly, however, a plea is made to place the ureter anterior to the graft and to study the patient both pre- and postoperatively to prevent obstruction of the ureter in these patients.", "contents": "Ureteral obstruction after aorto-femoral bypass surgery. A case of ureteral obstruction from bypass surgery is presented with review of the literature. A pathogenesis for the obstruction has been suggested and specific treatment recommended. More importantly, however, a plea is made to place the ureter anterior to the graft and to study the patient both pre- and postoperatively to prevent obstruction of the ureter in these patients."}
{"id": "PMID:483506", "title": "Intratesticular papillary mucinous cystadenocarcinoma.", "content": "The first case of intratesticular papillary mucinous cystadenocarcinoma is described. The tumor was histopathologically different from other types of papillary carcinoma related to the testicle and is believed to have originated from an intratesticular m\u00fcllerian duct remnant.", "contents": "Intratesticular papillary mucinous cystadenocarcinoma. The first case of intratesticular papillary mucinous cystadenocarcinoma is described. The tumor was histopathologically different from other types of papillary carcinoma related to the testicle and is believed to have originated from an intratesticular m\u00fcllerian duct remnant."}
{"id": "PMID:483507", "title": "Multifocal fibrous pseudotumor of testicular tunics. Possible clinical dilemma.", "content": "A morphologic characterization of a tunical multifocal fibrous pseudotumor is presented because of the clinical dilemma this rare entity causes urologists and pathologists. This report demonstrates the necessity for familiarity with testicular pseudotumors in order to avoid an unnecessary orchiectomy. If histopathologic examination of biopsies from every pseudotumor nodule fails to demonstrate malignancy, excision of the entire tunics including the pseudotumors is appropriate therapy.", "contents": "Multifocal fibrous pseudotumor of testicular tunics. Possible clinical dilemma. A morphologic characterization of a tunical multifocal fibrous pseudotumor is presented because of the clinical dilemma this rare entity causes urologists and pathologists. This report demonstrates the necessity for familiarity with testicular pseudotumors in order to avoid an unnecessary orchiectomy. If histopathologic examination of biopsies from every pseudotumor nodule fails to demonstrate malignancy, excision of the entire tunics including the pseudotumors is appropriate therapy."}
{"id": "PMID:483508", "title": "Unusual benign bladder tumor of Brunn nest origin.", "content": "An unusual bladder tumor consisting of proliferating benign Brunn nests is described. The tumor occurred in a forty-five-year-old man who presented with hematuria. The tumor recurred in the same location one and one-half years later and again caused hematuria. The tumor is distinguished from inverted papilloma of the bladder.", "contents": "Unusual benign bladder tumor of Brunn nest origin. An unusual bladder tumor consisting of proliferating benign Brunn nests is described. The tumor occurred in a forty-five-year-old man who presented with hematuria. The tumor recurred in the same location one and one-half years later and again caused hematuria. The tumor is distinguished from inverted papilloma of the bladder."}
{"id": "PMID:483510", "title": "Arterial embolization of bleeding pseudoaneurysm caused by percutaneous renal biopsy.", "content": "A pseudoaneurysm of a primary branch of the renal artery with massive hemorrhage developed in a patient who underwent percutaneous needle renal biopsy. He was treated successfully by embolization of the artery with gelatin sponge (Gelfoam).", "contents": "Arterial embolization of bleeding pseudoaneurysm caused by percutaneous renal biopsy. A pseudoaneurysm of a primary branch of the renal artery with massive hemorrhage developed in a patient who underwent percutaneous needle renal biopsy. He was treated successfully by embolization of the artery with gelatin sponge (Gelfoam)."}
{"id": "PMID:483511", "title": "Common origin of inferior mesenteric and accessory renal artery.", "content": "Arteriography performed in the preoperative evaluation of a patient with horseshoe kidney and renal calculi revealed a common origin of the inferior mesenteric artery and an anomalous left lower polar renal artery. The origin of the anomaly and its clinical significance are reviewed.", "contents": "Common origin of inferior mesenteric and accessory renal artery. Arteriography performed in the preoperative evaluation of a patient with horseshoe kidney and renal calculi revealed a common origin of the inferior mesenteric artery and an anomalous left lower polar renal artery. The origin of the anomaly and its clinical significance are reviewed."}
{"id": "PMID:483512", "title": "Clinical use of biocompatible ultrasonic bladder volume sensor.", "content": "A biocompatible ultrasonic bladder volume sensor has been developed and successfully employed to sense bladder fullness in healthy volunteers. It is anticipated that it will be of practical use in patients with diabetic autonomic neuropathy as well as in patients employing self-intermittent catheterization.", "contents": "Clinical use of biocompatible ultrasonic bladder volume sensor. A biocompatible ultrasonic bladder volume sensor has been developed and successfully employed to sense bladder fullness in healthy volunteers. It is anticipated that it will be of practical use in patients with diabetic autonomic neuropathy as well as in patients employing self-intermittent catheterization."}
{"id": "PMID:483531", "title": "Experience of the metabolisable energy system.", "content": "Advisory experience concerning the metabolisable energy standards for cattle and sheep introduced in 1976 is reviewed. The change appears to have proceeded smoothly, the new terminology and units being mastered and then used in discussions on dairy cow feeding. The system proposed has thrown new light on some old problems and also on established methods of feeding dairy cattle. \"Feeding according to yield\" and \"lead feeding\" need to be reinterpreted in the light of the probable dry matter intakes and live-weight changes of cows in early lactation. These parameters also affect the calculated levels of protein received in dairy compound feeds in early lactation. Group feeding of cows by stage of lactation, level of milk yield and live-weight change is readily accepted by managers of the larger dairy herds. Confidence in the accuracy of the dairy ME system has been built up, and its application to suckler cows has also proved successful. The variable net energy system for growing cattle enables ration formulation to be accomplished speedily and linear programming if desired. Published experiments have been used to confirm the accuracy of predicted live-weight gains when compared with observed gains. No sex effects are included in the system, although differences of +/- 10 per cent have been recorded in natural or hormone induced sex effect trials. The requirements for pregnant ewes have been shown to be too low and liable to reduce lamb birth-weights. In the case of growing lambs, a review of published experiments has shown that lambs grew faster than predicted, suggesting that the energy allowances are too high.", "contents": "Experience of the metabolisable energy system. Advisory experience concerning the metabolisable energy standards for cattle and sheep introduced in 1976 is reviewed. The change appears to have proceeded smoothly, the new terminology and units being mastered and then used in discussions on dairy cow feeding. The system proposed has thrown new light on some old problems and also on established methods of feeding dairy cattle. \"Feeding according to yield\" and \"lead feeding\" need to be reinterpreted in the light of the probable dry matter intakes and live-weight changes of cows in early lactation. These parameters also affect the calculated levels of protein received in dairy compound feeds in early lactation. Group feeding of cows by stage of lactation, level of milk yield and live-weight change is readily accepted by managers of the larger dairy herds. Confidence in the accuracy of the dairy ME system has been built up, and its application to suckler cows has also proved successful. The variable net energy system for growing cattle enables ration formulation to be accomplished speedily and linear programming if desired. Published experiments have been used to confirm the accuracy of predicted live-weight gains when compared with observed gains. No sex effects are included in the system, although differences of +/- 10 per cent have been recorded in natural or hormone induced sex effect trials. The requirements for pregnant ewes have been shown to be too low and liable to reduce lamb birth-weights. In the case of growing lambs, a review of published experiments has shown that lambs grew faster than predicted, suggesting that the energy allowances are too high."}
{"id": "PMID:483538", "title": "Pet health insurances and contract services: the potential for practice involvement.", "content": "Pet medical insurance has appreciable advantages and the existing policies appear to provide a beneficial service. But administrative costs of the small premiums involved make it of doubtful commercial attraction or benefit to the client. However, practice contract service schemes reduce administrative costs and can provide many of the advantages of insurance. Such a scheme is described. A coordinating agency outside the practice to produce promotional material, contracts, formulae for setting premiums and exclusions and to act as an arbitration agency for disputes could be established given the demand.", "contents": "Pet health insurances and contract services: the potential for practice involvement. Pet medical insurance has appreciable advantages and the existing policies appear to provide a beneficial service. But administrative costs of the small premiums involved make it of doubtful commercial attraction or benefit to the client. However, practice contract service schemes reduce administrative costs and can provide many of the advantages of insurance. Such a scheme is described. A coordinating agency outside the practice to produce promotional material, contracts, formulae for setting premiums and exclusions and to act as an arbitration agency for disputes could be established given the demand."}
{"id": "PMID:483539", "title": "Hormonal and histological studies in a 61XXY bull.", "content": "A Friesian bull with bilateral testicular hypoplasia was diagnosed as having a pure 61XXY karyotype. The bull displayed normal sexual behaviour but was azoospermic. At 17 months, the animal's peripheral plasma androgen levels appeared to be lower than normal, and one testicle removed one month later showed small seminiferous tubules totally lacking in germ cells. The Leydig cell volume of this testis was well within the normal range but the tubule length was rather short. At 33 months of age an increase in peripheral plasma androgen levels was noted. In the remaining testis there had been both a considerable rise in Leydig cell volume and a fall in tubule length. These findings may be explained by the interaction of three factors; the effects of the abnormal karyotype, the increased maturity of the animal at 33 months and the reaction of the remaining testis to unilateral castration.", "contents": "Hormonal and histological studies in a 61XXY bull. A Friesian bull with bilateral testicular hypoplasia was diagnosed as having a pure 61XXY karyotype. The bull displayed normal sexual behaviour but was azoospermic. At 17 months, the animal's peripheral plasma androgen levels appeared to be lower than normal, and one testicle removed one month later showed small seminiferous tubules totally lacking in germ cells. The Leydig cell volume of this testis was well within the normal range but the tubule length was rather short. At 33 months of age an increase in peripheral plasma androgen levels was noted. In the remaining testis there had been both a considerable rise in Leydig cell volume and a fall in tubule length. These findings may be explained by the interaction of three factors; the effects of the abnormal karyotype, the increased maturity of the animal at 33 months and the reaction of the remaining testis to unilateral castration."}
{"id": "PMID:483674", "title": "[Time of doubling of the volume of malignant tumors as a criterion of the effectiveness of their treament].", "content": "The authors have studied the rate of growth and determined the time for volume doubling in 31 primary and metastatic tumors. There was found a considerable time variation for the doubling of different tumors and identical tumors in different patients. In 15 patients the period of time during which multiple pulmonary metastases doubled their volume was determined. Considerable time variations were noted in 10 of them. It is suggested to use the variations in the time of doubling as an index of the therapy efficacy. There is a statistically reliable correlation between the time of the tumor volume being doubled and patients' survival.", "contents": "[Time of doubling of the volume of malignant tumors as a criterion of the effectiveness of their treament]. The authors have studied the rate of growth and determined the time for volume doubling in 31 primary and metastatic tumors. There was found a considerable time variation for the doubling of different tumors and identical tumors in different patients. In 15 patients the period of time during which multiple pulmonary metastases doubled their volume was determined. Considerable time variations were noted in 10 of them. It is suggested to use the variations in the time of doubling as an index of the therapy efficacy. There is a statistically reliable correlation between the time of the tumor volume being doubled and patients' survival."}
{"id": "PMID:483675", "title": "[Results of the surgical treatment of lung cancer].", "content": "Clinical observations and autopsy findings indicate that radical surgery for lung cancer is followed in most cases by tumor progression. The first signs of the progression would more frequently develop during two years postoperatively, and these were due to metastases in distal organs (49.6%) and lymph nodes (35.2%), more rarely due to the recurrence (11.2%) or implantation metastases (4.0%). According to atuopsy findings in patients, died in late terms after radical surgery, the signs of lung cancer progression were revealed in 87.9% of cases. Among these surgical therapy rendered no effect on the frequency and localization of metastases as compared with untreated patients.", "contents": "[Results of the surgical treatment of lung cancer]. Clinical observations and autopsy findings indicate that radical surgery for lung cancer is followed in most cases by tumor progression. The first signs of the progression would more frequently develop during two years postoperatively, and these were due to metastases in distal organs (49.6%) and lymph nodes (35.2%), more rarely due to the recurrence (11.2%) or implantation metastases (4.0%). According to atuopsy findings in patients, died in late terms after radical surgery, the signs of lung cancer progression were revealed in 87.9% of cases. Among these surgical therapy rendered no effect on the frequency and localization of metastases as compared with untreated patients."}
{"id": "PMID:483676", "title": "[Principles of the clinical study of new antitumor perparations].", "content": "Clinical studies on new antitumor drugs are characterized by a number of features different from testing other drugs and may be divided into 3 phases, each having its specific aims. Of greatest importance for adequate estimation of a new cytostatic is selecting a primary dosage and its increase, selecting of the so-called \"signal\" tumors, elaborating the regimen of using the drug which agrees with the mechanism of its action and pharmacokinetics. The methods of randomization and stratification may be used for the third phase, but some elements of the latter are essential for the second phase to compensate the effect of different factors (age and general patients' status, the degree of proliferation and intensity of the process) on the results of chemotherapy. To compare the results of clinical trials, it is necessary to have a unified system of estimating the effectiveness of new chemotherapeutic antitumor drugs.", "contents": "[Principles of the clinical study of new antitumor perparations]. Clinical studies on new antitumor drugs are characterized by a number of features different from testing other drugs and may be divided into 3 phases, each having its specific aims. Of greatest importance for adequate estimation of a new cytostatic is selecting a primary dosage and its increase, selecting of the so-called \"signal\" tumors, elaborating the regimen of using the drug which agrees with the mechanism of its action and pharmacokinetics. The methods of randomization and stratification may be used for the third phase, but some elements of the latter are essential for the second phase to compensate the effect of different factors (age and general patients' status, the degree of proliferation and intensity of the process) on the results of chemotherapy. To compare the results of clinical trials, it is necessary to have a unified system of estimating the effectiveness of new chemotherapeutic antitumor drugs."}
{"id": "PMID:483678", "title": "[Ultrastructural stereometry of highly differentiated transitional cell tumors of the bladder].", "content": "Cytomorphometric examination of the normal urothelium, 3 primary, 3 recurrent papillomas of the urinary bladder and 3 cancers of stage I indicated that average relative volumes of the nucleus and cytoplasm in their cells are nearly similar. Since these bolumes increase simultaneously in the tumors concerned, there is no change in the \"nucleus-cytoplasm correlation\" or \"index\" described above. Cytomorphometric indices of the nucleus and cytoplasma in papillomas are more close to those of high-differentiated cancer than of the normal urothelium. In histologically indistinguishable primary and recurrent transitional cell papillomas the indices would differ: in primary papillomas compared with the norma the amount of mitochondria is reliably increased statistically, but the volume of each of them is diminished, whereas in recurrent ones--vice versa.", "contents": "[Ultrastructural stereometry of highly differentiated transitional cell tumors of the bladder]. Cytomorphometric examination of the normal urothelium, 3 primary, 3 recurrent papillomas of the urinary bladder and 3 cancers of stage I indicated that average relative volumes of the nucleus and cytoplasm in their cells are nearly similar. Since these bolumes increase simultaneously in the tumors concerned, there is no change in the \"nucleus-cytoplasm correlation\" or \"index\" described above. Cytomorphometric indices of the nucleus and cytoplasma in papillomas are more close to those of high-differentiated cancer than of the normal urothelium. In histologically indistinguishable primary and recurrent transitional cell papillomas the indices would differ: in primary papillomas compared with the norma the amount of mitochondria is reliably increased statistically, but the volume of each of them is diminished, whereas in recurrent ones--vice versa."}
{"id": "PMID:483679", "title": "[Effect of sex and age on the frequency of tumors arising in nonlineal mice under total gamma irradiation].", "content": "Single total 1 beta 7Cs irradiation of mice of colony SHK increases the incidence of tumors in female animals. Ovarian tumors would develop irrespective of the mice age to the moment of irradiation. The increased frequency of appearance of tumors of the mammary glands was noted in older female animals. Radiation enhances the development of tumors of the hematopoietic system in female animals and hepatic and pulmonary tumors in males. An average life survival is decreased in young females exposed to the irradiation.", "contents": "[Effect of sex and age on the frequency of tumors arising in nonlineal mice under total gamma irradiation]. Single total 1 beta 7Cs irradiation of mice of colony SHK increases the incidence of tumors in female animals. Ovarian tumors would develop irrespective of the mice age to the moment of irradiation. The increased frequency of appearance of tumors of the mammary glands was noted in older female animals. Radiation enhances the development of tumors of the hematopoietic system in female animals and hepatic and pulmonary tumors in males. An average life survival is decreased in young females exposed to the irradiation."}
{"id": "PMID:483680", "title": "[Possible carcinogenic action of chromogen dicarboxidine].", "content": "In chronic experiments on 208 rats and 127 mice tne carcinogenic activity of dicarboxidine was examined. The substance was administered percutaneously and by leeding it during two years in the dosage: 20 mg -- 45 mg for rats, 3 mg -- 4 mg for mice. In late terms of the experiment (following one year and a half) the rats developed sarcomas at the site of its injection as well as tumors of other organs, including the urinary bladder. The substance was found to render no appreciable effect on mice. A conclusion is drawn on an insignificant carcinogenic activity of the substance under test, taking into account the late terms of tumors appearance and the induction of tumors only in one species of animals.", "contents": "[Possible carcinogenic action of chromogen dicarboxidine]. In chronic experiments on 208 rats and 127 mice tne carcinogenic activity of dicarboxidine was examined. The substance was administered percutaneously and by leeding it during two years in the dosage: 20 mg -- 45 mg for rats, 3 mg -- 4 mg for mice. In late terms of the experiment (following one year and a half) the rats developed sarcomas at the site of its injection as well as tumors of other organs, including the urinary bladder. The substance was found to render no appreciable effect on mice. A conclusion is drawn on an insignificant carcinogenic activity of the substance under test, taking into account the late terms of tumors appearance and the induction of tumors only in one species of animals."}
{"id": "PMID:483682", "title": "[Blastomogenic action of combined total x-ray irradiation and the administration of 131I on young rats].", "content": "The combined irradiation of rats, aged 36--42 days (the thyroid uptake--6900 rad) would induce the development of neoplasms characteristic of each factor exposure. The incidence of thyroid tumors (75.0%) was close to that induced by a separate 131I exposure (72.5%) the thyroid uptake being 6200 rad.", "contents": "[Blastomogenic action of combined total x-ray irradiation and the administration of 131I on young rats]. The combined irradiation of rats, aged 36--42 days (the thyroid uptake--6900 rad) would induce the development of neoplasms characteristic of each factor exposure. The incidence of thyroid tumors (75.0%) was close to that induced by a separate 131I exposure (72.5%) the thyroid uptake being 6200 rad."}
{"id": "PMID:483683", "title": "[Lactate dehydrogenase and malate dehydrogenase isoenzymes at different stages of the mitotic cycle in Ehrlich tumor cells].", "content": "LDH is represented in the tumor almost exclusively by an extremely slow migrating isozyme LDH-5. There is actually no LDH-1, LDH-2, LDH-3 in the spectrum, while LDH-4 is in small amounts. MDH is represented by cytoplasmic isozyme (S-MDH) and a mitochondrial fraction (m-MDH). The activity of LDH-5 and S-MDH is altered in a synchronous way: it shows a very sharp increase at the very beginning of the mitotic cycle and reaches the maximum value already by the mid-S-phase. The m-MDH activity through the cycle is enhanced twice: at the beginning of the cycle and following the termination of S-phase. There was found a direct correlation between the activity of m-MDH and the rate of anaerobic glycolysis through the mitotic cycle of Ehrlich tumor cells.", "contents": "[Lactate dehydrogenase and malate dehydrogenase isoenzymes at different stages of the mitotic cycle in Ehrlich tumor cells]. LDH is represented in the tumor almost exclusively by an extremely slow migrating isozyme LDH-5. There is actually no LDH-1, LDH-2, LDH-3 in the spectrum, while LDH-4 is in small amounts. MDH is represented by cytoplasmic isozyme (S-MDH) and a mitochondrial fraction (m-MDH). The activity of LDH-5 and S-MDH is altered in a synchronous way: it shows a very sharp increase at the very beginning of the mitotic cycle and reaches the maximum value already by the mid-S-phase. The m-MDH activity through the cycle is enhanced twice: at the beginning of the cycle and following the termination of S-phase. There was found a direct correlation between the activity of m-MDH and the rate of anaerobic glycolysis through the mitotic cycle of Ehrlich tumor cells."}
{"id": "PMID:483684", "title": "[Effect of the nucleic factor on lymphocyte cytotoxicity].", "content": "Under study was the influence of the nucleic factor, isolated from Ehrlich tumor ascitic fluid, on cytotoxicity of in vitro lymphocytes of CC57Br mice immunized by L-cells. In a dose of 5--10 mkm/ml this factor was shown to suppress cytotoxicity of splenic cells as well as that of lymph nodes cells of previously immunized mice. Cellular DNA of non-lineal SHR mice, hepatic RNA of normal mice C3Ha (their genotype being similar to L-cells), of non-lineal SHR mice and syngeneic CC75Br in the same concentration failed to produce such effect.", "contents": "[Effect of the nucleic factor on lymphocyte cytotoxicity]. Under study was the influence of the nucleic factor, isolated from Ehrlich tumor ascitic fluid, on cytotoxicity of in vitro lymphocytes of CC57Br mice immunized by L-cells. In a dose of 5--10 mkm/ml this factor was shown to suppress cytotoxicity of splenic cells as well as that of lymph nodes cells of previously immunized mice. Cellular DNA of non-lineal SHR mice, hepatic RNA of normal mice C3Ha (their genotype being similar to L-cells), of non-lineal SHR mice and syngeneic CC75Br in the same concentration failed to produce such effect."}
{"id": "PMID:483685", "title": "[Quantitative aspects of the relationship of the antitumor activity and toxicity of diaziridinyl-sym. triazines to their physicochemical properties].", "content": "The experiments on rats have shown that there is a linear dependence of the logarithms of reciprocal molar toxic (LD50) and therapeutic (ED50) for sarcoma 45 doses of 6-oxyalkyl-amino-2,4-bis(I-aziridinyl)-sym-triazines and 6-(5-substituted-2,2-dimethyl-1,3-dioxan-5-yl)-amino-2,4-bis(I-aziridinyl)-sym-triazines upon the induction constants of substituents at tertiary carbon, the antitumor activity being more sensitive to the substituent effect compared with the toxicity. The dependence of the logarithms of reciprocal molar therapeutic (ED50 for sarcoma 45 and ED95 for Walker sarcoma) and toxic (LD50 and LD100) doses of the compounds, both synthesized by us and taken from the literature, upon the logarithms of their distribution coefficients in the octanol-water system (IgP) shows a parabolic character with the maximum effect at the extreme point of the parabola. The antitumor activity was more sensitive to a changed IgP than the toxicity.", "contents": "[Quantitative aspects of the relationship of the antitumor activity and toxicity of diaziridinyl-sym. triazines to their physicochemical properties]. The experiments on rats have shown that there is a linear dependence of the logarithms of reciprocal molar toxic (LD50) and therapeutic (ED50) for sarcoma 45 doses of 6-oxyalkyl-amino-2,4-bis(I-aziridinyl)-sym-triazines and 6-(5-substituted-2,2-dimethyl-1,3-dioxan-5-yl)-amino-2,4-bis(I-aziridinyl)-sym-triazines upon the induction constants of substituents at tertiary carbon, the antitumor activity being more sensitive to the substituent effect compared with the toxicity. The dependence of the logarithms of reciprocal molar therapeutic (ED50 for sarcoma 45 and ED95 for Walker sarcoma) and toxic (LD50 and LD100) doses of the compounds, both synthesized by us and taken from the literature, upon the logarithms of their distribution coefficients in the octanol-water system (IgP) shows a parabolic character with the maximum effect at the extreme point of the parabola. The antitumor activity was more sensitive to a changed IgP than the toxicity."}
{"id": "PMID:483686", "title": "[Cryotherapy of precancerous diseases of the cervix uteri].", "content": "In the COR of the USSR Academy of Medical Sciences cryotherapy was employed in 54 patients with pretumor lesions of the uterine cervix. The cryotherapy was performed by a \"Spembly\" machine (England) and a national apparatus. As a cryoagent nitrogen monoxide was used, which provided the cooling of a cryosonde up to -89 degrees C. This method offers such advantages as the absence of pain, bleeding and complications. An analysis of the immediate results of the treatment allows cryoexposure to be considered an effective method and to employ it under out-patient conditions.", "contents": "[Cryotherapy of precancerous diseases of the cervix uteri]. In the COR of the USSR Academy of Medical Sciences cryotherapy was employed in 54 patients with pretumor lesions of the uterine cervix. The cryotherapy was performed by a \"Spembly\" machine (England) and a national apparatus. As a cryoagent nitrogen monoxide was used, which provided the cooling of a cryosonde up to -89 degrees C. This method offers such advantages as the absence of pain, bleeding and complications. An analysis of the immediate results of the treatment allows cryoexposure to be considered an effective method and to employ it under out-patient conditions."}
{"id": "PMID:483687", "title": "[Experience with the organization of prophylactic oncological examinations].", "content": "Mass prophylactic examinations among the population to early recognize oncological diseases may be perfected in the following way: by making them less time-consuming, regular and actually total. To this end they should be available for everybody referring to therapeutic specialists during a year, an appointment with the doctor is to be proceeded by a fluorographic check, while in females--by a visit to the women's diagnostic department. For this examinations to be really total as well as to exclude the cases of double examination the record of each person should be kept.", "contents": "[Experience with the organization of prophylactic oncological examinations]. Mass prophylactic examinations among the population to early recognize oncological diseases may be perfected in the following way: by making them less time-consuming, regular and actually total. To this end they should be available for everybody referring to therapeutic specialists during a year, an appointment with the doctor is to be proceeded by a fluorographic check, while in females--by a visit to the women's diagnostic department. For this examinations to be really total as well as to exclude the cases of double examination the record of each person should be kept."}
{"id": "PMID:483688", "title": "[Tumors in children].", "content": "Biopsy and autopsy findings obtained at the Tartu autopsy department for a 35-year period (from 1941 to 1975) are analysed. Within this period tumors in children under 16 years of age were observed in 1183 cases. Benign tumors (970) were predominating over malignant ones (213). Among benign tumors hemangiomas were in the first place and more frequently observed in girls. Among malignant tumors sarcomas were most frequently detected. Malignant tumors in girls were as frequent as in boys.", "contents": "[Tumors in children]. Biopsy and autopsy findings obtained at the Tartu autopsy department for a 35-year period (from 1941 to 1975) are analysed. Within this period tumors in children under 16 years of age were observed in 1183 cases. Benign tumors (970) were predominating over malignant ones (213). Among benign tumors hemangiomas were in the first place and more frequently observed in girls. Among malignant tumors sarcomas were most frequently detected. Malignant tumors in girls were as frequent as in boys."}
{"id": "PMID:483689", "title": "[Endometrioid ovarian neoplasms with epidermoid structures].", "content": "Under study was the intraoperative material from 32 patients with endometrial ovarian neoplasms having an epidermoid structure. Epidermoid foci were found to be pathognomonic only for the endometrioid epithelium. This fact may be used for the differential diagnosis with serous neoplasms. Biologically adenoacanthomas may be qualified as borderline processes, i.e. potentially a low grade of malignancy.", "contents": "[Endometrioid ovarian neoplasms with epidermoid structures]. Under study was the intraoperative material from 32 patients with endometrial ovarian neoplasms having an epidermoid structure. Epidermoid foci were found to be pathognomonic only for the endometrioid epithelium. This fact may be used for the differential diagnosis with serous neoplasms. Biologically adenoacanthomas may be qualified as borderline processes, i.e. potentially a low grade of malignancy."}
{"id": "PMID:483695", "title": "[Complex x-ray, gastroscopic and cytological diagnosis of malignant gastric ulcer].", "content": "Under discussion are some aspects of the diagnosis of malignant gastric ulcers established by means of a complex gastrologic examination of patients with ulcerous pathology of the stomach. Errors, difficulties and possibilities of roentgenological, gastroscopic and cytologic methods for recognition of malignant gastric ulcers are analysed. It is emphasized that cytological assay is highly efficient in ascertaining a malignant nature of the ulcer. A careful complex investigation of patient with gastric ulcer is felt to be indispensable to recognize its early stages.", "contents": "[Complex x-ray, gastroscopic and cytological diagnosis of malignant gastric ulcer]. Under discussion are some aspects of the diagnosis of malignant gastric ulcers established by means of a complex gastrologic examination of patients with ulcerous pathology of the stomach. Errors, difficulties and possibilities of roentgenological, gastroscopic and cytologic methods for recognition of malignant gastric ulcers are analysed. It is emphasized that cytological assay is highly efficient in ascertaining a malignant nature of the ulcer. A careful complex investigation of patient with gastric ulcer is felt to be indispensable to recognize its early stages."}
{"id": "PMID:483696", "title": "[Use of large-frame fluorography in the diagnosis of stomach cancer].", "content": "A comparison of the results of non-aimed large-frame fluorography (LFF) and roentgen study of the stomach in 301 patients enabled ascertaining more precisely the opportunities of LFF in diagnosing gastric cancer. It was found by checking (fibrogastroscopy with biopsy or surger) that irrespective of the technic of non-aimed LFF, despite the diagnosis being known previously, cancer of the stomach still far from being in early stage may be recognized only in 62-63%. Therefore use of non-aimed LFF to diagnose gastric cancer during mass prophylactic examinations seems to be not rational.", "contents": "[Use of large-frame fluorography in the diagnosis of stomach cancer]. A comparison of the results of non-aimed large-frame fluorography (LFF) and roentgen study of the stomach in 301 patients enabled ascertaining more precisely the opportunities of LFF in diagnosing gastric cancer. It was found by checking (fibrogastroscopy with biopsy or surger) that irrespective of the technic of non-aimed LFF, despite the diagnosis being known previously, cancer of the stomach still far from being in early stage may be recognized only in 62-63%. Therefore use of non-aimed LFF to diagnose gastric cancer during mass prophylactic examinations seems to be not rational."}
{"id": "PMID:483697", "title": "[Incidence of stomach cancer in the USSR].", "content": "The authors have studied some tendencies in the morbidity and mortality due to malignant neoplasms of the stomach for the period from 1970 to 1975. The age-sex indices of the morbidity are reported as well as some data on a contingency of gastric cancer patients under dispensary control. There is noted a reduced morbidity and mortality rate due to gastric cancer among the population of this country.", "contents": "[Incidence of stomach cancer in the USSR]. The authors have studied some tendencies in the morbidity and mortality due to malignant neoplasms of the stomach for the period from 1970 to 1975. The age-sex indices of the morbidity are reported as well as some data on a contingency of gastric cancer patients under dispensary control. There is noted a reduced morbidity and mortality rate due to gastric cancer among the population of this country."}
{"id": "PMID:483698", "title": "[Oxidoreductase activity in the cells of stomach cancer].", "content": "NAD- and NADP-dependent dehydrogenases in gastric adenocarcinoma and undifferentiated cancer cells were studied comparatively. The activity of NADP-dependent malate dehydrogenase, glutamate dehydrogenase and glucose-6-phosphate dehydrogenase was found to be high in gastric adenocarcinoma, while there was noted a more high activity of succinate dehydrogenase and NAD-dependent malate dehydrogenase in undifferentiated cancer. Differences ni the activity of oxido-reductive enzymes in adrenocarcinoma and undifferentiated cancer are discussed from the standpoint of various histogenesis of these forms of gastric cancer.", "contents": "[Oxidoreductase activity in the cells of stomach cancer]. NAD- and NADP-dependent dehydrogenases in gastric adenocarcinoma and undifferentiated cancer cells were studied comparatively. The activity of NADP-dependent malate dehydrogenase, glutamate dehydrogenase and glucose-6-phosphate dehydrogenase was found to be high in gastric adenocarcinoma, while there was noted a more high activity of succinate dehydrogenase and NAD-dependent malate dehydrogenase in undifferentiated cancer. Differences ni the activity of oxido-reductive enzymes in adrenocarcinoma and undifferentiated cancer are discussed from the standpoint of various histogenesis of these forms of gastric cancer."}
{"id": "PMID:483699", "title": "[Cancer of the greater curvature of the stomach].", "content": "The analysis of 102 observations indicated the clinico-roentgenological features of cancer of the greater curvature of the stomach, thus enabling the possibilities of a roentgenological method of examination to be ascertained in the complex of methods for examining patients with the pathology involved. It was noted that in cancer of the larger curvature of the stomach the character of the lesion is not infrequently masked by the symptoms observed in ulcerous disease, chronic gastritis or Menetrie disease. Accessory methods of roentgenodiagnosis: pneumogastrography, double and triple contrasting technic contribute to pinpointing the true character of the process. Application of the mentioned technics allowed the precise diagnosis to be established in 87.2% of cases. When using fibrogastroscopy the correct diagnosis was made in 86.5% of patients. Use of the complex examination allowed the precise preoperative diagnosis in 92.1%. Some problems of the differential diagnosis between cancer of the larger curvature of the stomach and ulcerous gastric disease are discussed.", "contents": "[Cancer of the greater curvature of the stomach]. The analysis of 102 observations indicated the clinico-roentgenological features of cancer of the greater curvature of the stomach, thus enabling the possibilities of a roentgenological method of examination to be ascertained in the complex of methods for examining patients with the pathology involved. It was noted that in cancer of the larger curvature of the stomach the character of the lesion is not infrequently masked by the symptoms observed in ulcerous disease, chronic gastritis or Menetrie disease. Accessory methods of roentgenodiagnosis: pneumogastrography, double and triple contrasting technic contribute to pinpointing the true character of the process. Application of the mentioned technics allowed the precise diagnosis to be established in 87.2% of cases. When using fibrogastroscopy the correct diagnosis was made in 86.5% of patients. Use of the complex examination allowed the precise preoperative diagnosis in 92.1%. Some problems of the differential diagnosis between cancer of the larger curvature of the stomach and ulcerous gastric disease are discussed."}
{"id": "PMID:483700", "title": "[Clinical x-ray diagnosis of gastric leiomyomas].", "content": "Under observation were 23 patients with leiomyomas of the stomach. Leiomyomas were more frequently observed in females, in the proximal segment these were as frequent as in the distal one, but more frequently in the posterior wall. Submucous endo gastric localization was typical for most leiomyomas. The possibilities of clinico-roentgenological diagnosis were studied. The clinical picture of leiomyomas of the stomach shows pains in the epigastric region, general disturbances, acute profuse gastric hemorrhages. The most typical roentgenological sign of gastric leiomyoma is an oval or round filling defect with even margins around 5-6 cm in size. Not infrequently there are one or several ulcerations on the leiomyoma surface like \"a niche\" of oval or semioval shape. The mucosa folds around the filling defect are moved apart but not destroyed. Some information on gastroscopic examination of 8 patients with gastric leiomyomas is reported.", "contents": "[Clinical x-ray diagnosis of gastric leiomyomas]. Under observation were 23 patients with leiomyomas of the stomach. Leiomyomas were more frequently observed in females, in the proximal segment these were as frequent as in the distal one, but more frequently in the posterior wall. Submucous endo gastric localization was typical for most leiomyomas. The possibilities of clinico-roentgenological diagnosis were studied. The clinical picture of leiomyomas of the stomach shows pains in the epigastric region, general disturbances, acute profuse gastric hemorrhages. The most typical roentgenological sign of gastric leiomyoma is an oval or round filling defect with even margins around 5-6 cm in size. Not infrequently there are one or several ulcerations on the leiomyoma surface like \"a niche\" of oval or semioval shape. The mucosa folds around the filling defect are moved apart but not destroyed. Some information on gastroscopic examination of 8 patients with gastric leiomyomas is reported."}
{"id": "PMID:483701", "title": "[Cancer of the stump of the stomach].", "content": "In analysis of the mortality causes in the patients, previously subjected to gastric resection for ulcerous disease, it was found that the specific weight of the tumor of the remaining gastric portion in females was 2 times as much as in males (19.5% and 10.1% according to the autopsy findings, 10.2% and 6.8% as shown by the followup materials). There were revealed a number of factors causing these differences, the principal of them being: 1) predominance of senile subjects among the female group, 2) a later occurrence of gastroduodenal ulcers in them, 3) predominance of gastric ulcers over duodenal ones, 4) a longer survival in gastrectomized patients.", "contents": "[Cancer of the stump of the stomach]. In analysis of the mortality causes in the patients, previously subjected to gastric resection for ulcerous disease, it was found that the specific weight of the tumor of the remaining gastric portion in females was 2 times as much as in males (19.5% and 10.1% according to the autopsy findings, 10.2% and 6.8% as shown by the followup materials). There were revealed a number of factors causing these differences, the principal of them being: 1) predominance of senile subjects among the female group, 2) a later occurrence of gastroduodenal ulcers in them, 3) predominance of gastric ulcers over duodenal ones, 4) a longer survival in gastrectomized patients."}
{"id": "PMID:483702", "title": "[Extensive, combined and extensive-combined operations in cancer of the cardial portion of the stomach].", "content": "The authors present an analysis of the materials obtained in the clinic during a 21-year period. Among 438 patients subjected to radical surgery for cancer of the gastric cardia in 319 cases extensive, combined and extensive-combined surgical procedures were accomplished, their characteristics being described. The regular feature of cancer of this localization is its lymphogenic spread, and this fact together with esophageal involvement allows a consideration that for cancer of the cardia, in any stage but 1, all resections, as a rule, should be extensive, combined or both. The success of the immediate results of the operation depends on adequacy of an approach, kind of operation and extent of resection combinations in relation to the characteristic of cancer spread according to TNM system and the degree of surgical risk for each individual case. Estimation of the immediate and late results should take into account the motivation for performing extensive, combined and extensive-combined procedures. In cancer of the gastric cardia the analysed surgical procedures are to be referred to a group of conventionally radical interventions.", "contents": "[Extensive, combined and extensive-combined operations in cancer of the cardial portion of the stomach]. The authors present an analysis of the materials obtained in the clinic during a 21-year period. Among 438 patients subjected to radical surgery for cancer of the gastric cardia in 319 cases extensive, combined and extensive-combined surgical procedures were accomplished, their characteristics being described. The regular feature of cancer of this localization is its lymphogenic spread, and this fact together with esophageal involvement allows a consideration that for cancer of the cardia, in any stage but 1, all resections, as a rule, should be extensive, combined or both. The success of the immediate results of the operation depends on adequacy of an approach, kind of operation and extent of resection combinations in relation to the characteristic of cancer spread according to TNM system and the degree of surgical risk for each individual case. Estimation of the immediate and late results should take into account the motivation for performing extensive, combined and extensive-combined procedures. In cancer of the gastric cardia the analysed surgical procedures are to be referred to a group of conventionally radical interventions."}
{"id": "PMID:483703", "title": "[Prospects of the development of research into regulating chemical carcinogens in the environment].", "content": "The possibility of regulating the permissible quantities of chemical carcinogens in the environment is being substantiated. The ways of determining the threshold amounts of blastomogenic agents are discussed. Some approaches to the regulation of blastomogens are suggested depending on the degree of risk, effective quantities and action time. The main trends of development of researches in the field of regulation of chemical blastomogens are delineated.", "contents": "[Prospects of the development of research into regulating chemical carcinogens in the environment]. The possibility of regulating the permissible quantities of chemical carcinogens in the environment is being substantiated. The ways of determining the threshold amounts of blastomogenic agents are discussed. Some approaches to the regulation of blastomogens are suggested depending on the degree of risk, effective quantities and action time. The main trends of development of researches in the field of regulation of chemical blastomogens are delineated."}
{"id": "PMID:483704", "title": "[Induction of gastrointestinal tract tumors in rats with N-methyl-N-nitro-N-nitrosoguanidine (MNNG)].", "content": "Tumors of the gastrointestinal tract were induced in white non-inbred rats exposed to MNNG in various doses. Gastric tumors appeared in the dosage of 153 mg, with its 2 and 3.3 times increase no change in the frequency of gastric tumors was noted. The frequency of jejunal tumors was higher with increased MNNG dosage.", "contents": "[Induction of gastrointestinal tract tumors in rats with N-methyl-N-nitro-N-nitrosoguanidine (MNNG)]. Tumors of the gastrointestinal tract were induced in white non-inbred rats exposed to MNNG in various doses. Gastric tumors appeared in the dosage of 153 mg, with its 2 and 3.3 times increase no change in the frequency of gastric tumors was noted. The frequency of jejunal tumors was higher with increased MNNG dosage."}
{"id": "PMID:483705", "title": "[Effect of UHF frequency on the growth of sarcoma 180].", "content": "Studies performed on mice transplanted sarcoma 180 subcutaneously indicated that the UHF-field of non-thermogenic intensity in multiple (5-10 fold) total irradiation inhibits the tumor growth (T = 60%) and produces a 1.5 times increase of an average survival in tumor-bearing animals. It is suggested that there is a direct relationship between the increased antiblastic function of the body and a stimulating effect of the UHF-field on the immune response in animals.", "contents": "[Effect of UHF frequency on the growth of sarcoma 180]. Studies performed on mice transplanted sarcoma 180 subcutaneously indicated that the UHF-field of non-thermogenic intensity in multiple (5-10 fold) total irradiation inhibits the tumor growth (T = 60%) and produces a 1.5 times increase of an average survival in tumor-bearing animals. It is suggested that there is a direct relationship between the increased antiblastic function of the body and a stimulating effect of the UHF-field on the immune response in animals."}
{"id": "PMID:483712", "title": "[In vitro humeral serum cytotoxic reactions in melanoma].", "content": "Humoral antitumor cytotoxins were found in most of assayed sera from melanoma patients. The patients' sera can cross-react in cytotoxic reactions in vitro with allogenic cell-targets of melanoma. The sera of healthy donors prove to be cytotoxic for melanoma cell-targets in vitro, the degree of their cytotoxicity being similar to that of autologous sera. The cytotoxic effect of melanoma patient sera (and of healthy donors) on the cells of the primary melanoma focus is found to be reliably higher than their cytotoxic effect on metastatic cells.", "contents": "[In vitro humeral serum cytotoxic reactions in melanoma]. Humoral antitumor cytotoxins were found in most of assayed sera from melanoma patients. The patients' sera can cross-react in cytotoxic reactions in vitro with allogenic cell-targets of melanoma. The sera of healthy donors prove to be cytotoxic for melanoma cell-targets in vitro, the degree of their cytotoxicity being similar to that of autologous sera. The cytotoxic effect of melanoma patient sera (and of healthy donors) on the cells of the primary melanoma focus is found to be reliably higher than their cytotoxic effect on metastatic cells."}
{"id": "PMID:483713", "title": "[Electron microscopy diagnosis of soft tissue sarcoma].", "content": "The results of electron microscopy studies of 20 soft tissue sarcomas difficult to diagnose-fibrosarcomas, malignant fibroxanthomas, leiomyosarcomas, rhabdomyosarcomas and malignant schwannomas, are reported. The tumor elements of each of these neoplasms showed rather typical ultrastructural features enabling us to reliably ascertain their histological origin.", "contents": "[Electron microscopy diagnosis of soft tissue sarcoma]. The results of electron microscopy studies of 20 soft tissue sarcomas difficult to diagnose-fibrosarcomas, malignant fibroxanthomas, leiomyosarcomas, rhabdomyosarcomas and malignant schwannomas, are reported. The tumor elements of each of these neoplasms showed rather typical ultrastructural features enabling us to reliably ascertain their histological origin."}
{"id": "PMID:483714", "title": "[Mitotic regime of various spindle cell sarcomas and their differential diagnosis].", "content": "The mitotic regime was studied in 34 spindle-cell sarcomas of soft tissues, including 11 synovial, 13 low-differentiated fibrosarcomas, 5 leiomyosarcomas and 5 rhabdomyosarcomas. The compared neoplasms were found to differ in the degree of cell proliferation disorders (the mitotic activity, a preponderance of dividing cells at the stage of metaphase, the frequency of pathological mitoses and correlation of their different forms). It is shown that bridges are one of the features of the mitotic pathology for spindle-cell sarcomas, moreover myogenic sarcomas are characterized by a higher incidence of those mitotic disorders which occurrence is conditioned due to damaging of the mitotic apparatus (three group, multiple metaphases, c-mitoses, multipolar and monocentric mitoses).", "contents": "[Mitotic regime of various spindle cell sarcomas and their differential diagnosis]. The mitotic regime was studied in 34 spindle-cell sarcomas of soft tissues, including 11 synovial, 13 low-differentiated fibrosarcomas, 5 leiomyosarcomas and 5 rhabdomyosarcomas. The compared neoplasms were found to differ in the degree of cell proliferation disorders (the mitotic activity, a preponderance of dividing cells at the stage of metaphase, the frequency of pathological mitoses and correlation of their different forms). It is shown that bridges are one of the features of the mitotic pathology for spindle-cell sarcomas, moreover myogenic sarcomas are characterized by a higher incidence of those mitotic disorders which occurrence is conditioned due to damaging of the mitotic apparatus (three group, multiple metaphases, c-mitoses, multipolar and monocentric mitoses)."}
{"id": "PMID:483715", "title": "[Basement membranes in precancerous conditions and neoplasms of the uterine cervix].", "content": "By routine histological staining technics and histochemical assays under an appropriate enzymic control the author has studied changes in the basement membranes of the uterine cervix in its precancer and cancer as compared with the normal state. Changes in the basement membranes, revealed morphologically, reflect objectively the status of the epithelium and stroma during the period of tumor origination and growth, and therefore these may serve as a valuable adjunct to the differential microscopic diagnosis of dysplasia, preinvasive cancer and an incipient invasive growth. It is believed that basement membrane neoplasms may develop around mostly differentiated complexes of cancer cells in far-advanced neoplasms and their metastases; this fact is regarded as a tendency to normalization of the relationship between the cancerous tumor parenchyma and stroma with correlations between them being remined even under extremely unfavourable conditions of the tumor growth.", "contents": "[Basement membranes in precancerous conditions and neoplasms of the uterine cervix]. By routine histological staining technics and histochemical assays under an appropriate enzymic control the author has studied changes in the basement membranes of the uterine cervix in its precancer and cancer as compared with the normal state. Changes in the basement membranes, revealed morphologically, reflect objectively the status of the epithelium and stroma during the period of tumor origination and growth, and therefore these may serve as a valuable adjunct to the differential microscopic diagnosis of dysplasia, preinvasive cancer and an incipient invasive growth. It is believed that basement membrane neoplasms may develop around mostly differentiated complexes of cancer cells in far-advanced neoplasms and their metastases; this fact is regarded as a tendency to normalization of the relationship between the cancerous tumor parenchyma and stroma with correlations between them being remined even under extremely unfavourable conditions of the tumor growth."}
{"id": "PMID:483716", "title": "[Diagnosis and surgical treatment of carotid body chemodectoma].", "content": "The authors present an analysis of clinical, angiographic and operation finginds in 11 patients showing large chemodectomas with involvement of the common, external and internal carotid arteries and jugular vein. The carotid body angiography was found to be of great diagnostic value. In the presence of a patent internal carotid in 10 cases radical chemodectomy was performed, preserving the patent internal carotid and the vein. In case of a pathologically convoluted internal carotid the common carotid artery was resected with an end-to-end anastomosis. Late results were favourable even in patients with histological signs of an incipient malignant transformation of chemodectomy.", "contents": "[Diagnosis and surgical treatment of carotid body chemodectoma]. The authors present an analysis of clinical, angiographic and operation finginds in 11 patients showing large chemodectomas with involvement of the common, external and internal carotid arteries and jugular vein. The carotid body angiography was found to be of great diagnostic value. In the presence of a patent internal carotid in 10 cases radical chemodectomy was performed, preserving the patent internal carotid and the vein. In case of a pathologically convoluted internal carotid the common carotid artery was resected with an end-to-end anastomosis. Late results were favourable even in patients with histological signs of an incipient malignant transformation of chemodectomy."}
{"id": "PMID:483717", "title": "[Cytodiagnosis of endometrial cancer].", "content": "Uterine aspiration specimens from 172 females were examined cytologically (cancer in 89, non-cancer process -- in 71, in 12 cases aspiration specimens were obtained in a reproductive period in proliferative and secretory phases of the menstrual cycle). It was shown that in a group of histologically verified observations in 45.8% a gynecologist failed to recognize the true endometrial affection. Some cytological criteria elaborated are suggested (dominant, adjuvant and temporary), which combination helped to precisely diagnose cancer cytologically in 93.2%, while various forms of hyperplasia -- in 89.9%.", "contents": "[Cytodiagnosis of endometrial cancer]. Uterine aspiration specimens from 172 females were examined cytologically (cancer in 89, non-cancer process -- in 71, in 12 cases aspiration specimens were obtained in a reproductive period in proliferative and secretory phases of the menstrual cycle). It was shown that in a group of histologically verified observations in 45.8% a gynecologist failed to recognize the true endometrial affection. Some cytological criteria elaborated are suggested (dominant, adjuvant and temporary), which combination helped to precisely diagnose cancer cytologically in 93.2%, while various forms of hyperplasia -- in 89.9%."}
{"id": "PMID:483718", "title": "[Properties of viruses isolated from human fibroblasts culture treated by paraganglioma culture filtrate].", "content": "In the human fibroblasts culture, transformed by the material from malignant paraganglioma culture, there was found the RNA- and DNA containing virus synthesis. The RNA-containing virus with a buoyant density of 1.16--1.18 g/ml shows no hemagglutinating activity, it fails to infect newborn Syrian hamsters, and produces no transformation-destruction changes in mammalian tissue culture. The DNA-containing virus with a buoyant density of 1.24--1.28 g/ml would agglutinate the guinea-pig and group 0 human erythrocytes, produces lesions in newborn Syrian hamsters, and cytodestructive changes in the culture of rat and mice embryonal cells and in cells of a transplantable line Vero. In terms of its properties it may be referred to human viruses of the Papova group. A combined action of DNA- and RNA-containing viruses produces a transformation effect on embryonal hamster cells and the cells of a transplantable line Vero.", "contents": "[Properties of viruses isolated from human fibroblasts culture treated by paraganglioma culture filtrate]. In the human fibroblasts culture, transformed by the material from malignant paraganglioma culture, there was found the RNA- and DNA containing virus synthesis. The RNA-containing virus with a buoyant density of 1.16--1.18 g/ml shows no hemagglutinating activity, it fails to infect newborn Syrian hamsters, and produces no transformation-destruction changes in mammalian tissue culture. The DNA-containing virus with a buoyant density of 1.24--1.28 g/ml would agglutinate the guinea-pig and group 0 human erythrocytes, produces lesions in newborn Syrian hamsters, and cytodestructive changes in the culture of rat and mice embryonal cells and in cells of a transplantable line Vero. In terms of its properties it may be referred to human viruses of the Papova group. A combined action of DNA- and RNA-containing viruses produces a transformation effect on embryonal hamster cells and the cells of a transplantable line Vero."}
{"id": "PMID:483719", "title": "[Sebaceous gland reaction as a test method in the rapid determination of the blastomogenic activity of chemical substances].", "content": "The comprehensive experimental materials have shown that the sebaceous glands response to the action of carcinogenic substances is the most specific one since it develops within a short period of time and is revealed under the action of relatively small doses. Some practical recommendations are given as to the use of the sebaceous glands reaction as a tentative test for rapid detection of carcinogenic substances.", "contents": "[Sebaceous gland reaction as a test method in the rapid determination of the blastomogenic activity of chemical substances]. The comprehensive experimental materials have shown that the sebaceous glands response to the action of carcinogenic substances is the most specific one since it develops within a short period of time and is revealed under the action of relatively small doses. Some practical recommendations are given as to the use of the sebaceous glands reaction as a tentative test for rapid detection of carcinogenic substances."}
{"id": "PMID:483720", "title": "[Surgical treatment of pelvic and femoral bones neoplasms].", "content": "The author has reported 42 operations on the pelvic girdle and femoral bones. Two new variants of surgical intervention are suggested: 1) interiliac-pubic-sciatic amputation of a lower extremity, and 2) extended exarticulation of the femur. Des keletization of pelvic bones in both operative procedures renders them more ablastic and radical as compared with routine operations. Interiliac-abdominal resection is felt to be more rational from the rehabilitation viewpoint, moreover it is the author's opinion that resection of the femoral proximal segment should be avoided whenever possible.", "contents": "[Surgical treatment of pelvic and femoral bones neoplasms]. The author has reported 42 operations on the pelvic girdle and femoral bones. Two new variants of surgical intervention are suggested: 1) interiliac-pubic-sciatic amputation of a lower extremity, and 2) extended exarticulation of the femur. Des keletization of pelvic bones in both operative procedures renders them more ablastic and radical as compared with routine operations. Interiliac-abdominal resection is felt to be more rational from the rehabilitation viewpoint, moreover it is the author's opinion that resection of the femoral proximal segment should be avoided whenever possible."}
{"id": "PMID:483727", "title": "[Effect of the supplementary administration of vitamins in the ration on metabolic indices in people under alpine conditions].", "content": "It was shown that polyvitamin supplemented diets in which animal proteins had the biggest share and part of malassimilated polysaccharides had been replaced by easily assimilated disaccharides, promoted maintenance of the higher A/G ratio, low blood concentrations of lactic and pyruvic acids, and improvement of the parameters of acid-base metabolism in the people living under alpine conditions. After veloergometric test this group showed a less increase in the blood lactic acid content.", "contents": "[Effect of the supplementary administration of vitamins in the ration on metabolic indices in people under alpine conditions]. It was shown that polyvitamin supplemented diets in which animal proteins had the biggest share and part of malassimilated polysaccharides had been replaced by easily assimilated disaccharides, promoted maintenance of the higher A/G ratio, low blood concentrations of lactic and pyruvic acids, and improvement of the parameters of acid-base metabolism in the people living under alpine conditions. After veloergometric test this group showed a less increase in the blood lactic acid content."}
{"id": "PMID:483728", "title": "[Role of vitamin B6 in treating children with hereditary metabolic pathology].", "content": "Possibilities of vitamin B6 treatment of patients with hereditary pathology of metabolism are discussed. Special attention is paid to the vitamin B6-dependent conditions characterized by elevated pyridoxine requirements. High pyridoxine doses were successfully used for the treatment of patients with hereditary vitamin B6-dependent xanthurenuria under the control of renal excretion of tryptophan metabolites (xanthurenic and kynurenic acids, kynurenin, N1-methylnicotinamide) and 4-pyridoxic acid. Interrelation was traced between the severity of the disease and the necessary pyridoxine doses. Patients with the most pronounced clinical and biochemical changes needed especially high doses of the vitamin (200 mg/day). Use of vitamin B6 in a dose of 100 mg/day in pyridoxine-dependent homocystinuria induced a remission of the biochemical parameters on the 4th day of the treatment. It is noted that the efficacy of the treatment is dependent on the timely starting of the therapy and pyridoxine doses necessary for normalization of the clinical and biochemical parameters in each patient.", "contents": "[Role of vitamin B6 in treating children with hereditary metabolic pathology]. Possibilities of vitamin B6 treatment of patients with hereditary pathology of metabolism are discussed. Special attention is paid to the vitamin B6-dependent conditions characterized by elevated pyridoxine requirements. High pyridoxine doses were successfully used for the treatment of patients with hereditary vitamin B6-dependent xanthurenuria under the control of renal excretion of tryptophan metabolites (xanthurenic and kynurenic acids, kynurenin, N1-methylnicotinamide) and 4-pyridoxic acid. Interrelation was traced between the severity of the disease and the necessary pyridoxine doses. Patients with the most pronounced clinical and biochemical changes needed especially high doses of the vitamin (200 mg/day). Use of vitamin B6 in a dose of 100 mg/day in pyridoxine-dependent homocystinuria induced a remission of the biochemical parameters on the 4th day of the treatment. It is noted that the efficacy of the treatment is dependent on the timely starting of the therapy and pyridoxine doses necessary for normalization of the clinical and biochemical parameters in each patient."}
{"id": "PMID:483729", "title": "[Effect of diets with a varying protein and vitamin D content on the lipid makeup of the liver and blood serum].", "content": "Separate and combined effect of D hypovitaminosis and poor protein diet on the parameters of lipid metabolism in the blood serum and liver was investigated experimentally in albino rats. Increased changes in the blood serum lipid metabolism parameters in combined action of the nutrients deficiency as compared with the effect of alimentary factors were demonstrated. The lipid changes in the liver of the test animals in simultaneous deficiency of protein and vitamin D were similar to those in protein deficiency.", "contents": "[Effect of diets with a varying protein and vitamin D content on the lipid makeup of the liver and blood serum]. Separate and combined effect of D hypovitaminosis and poor protein diet on the parameters of lipid metabolism in the blood serum and liver was investigated experimentally in albino rats. Increased changes in the blood serum lipid metabolism parameters in combined action of the nutrients deficiency as compared with the effect of alimentary factors were demonstrated. The lipid changes in the liver of the test animals in simultaneous deficiency of protein and vitamin D were similar to those in protein deficiency."}
{"id": "PMID:483730", "title": "[Ultrastructure of the rat gastric mucosal parietal cells in thiamine deficiency].", "content": "The effect of the thiamine deficient diet on the ultrastructure and secretory response of parietal cells of the rat gastric mucosa was studied. The control group consisting of fasting animals showed 60% of the parietal cells to be in a presecretory state. Subcutaneous administration of histamine to the control animals was accompanied by secretion of 512 +/- 128 mkekv H+ over 3 hours, with only 18% of the parietal cells remaining not involved in the secretory process. In thiamine-free diets the number of secreting cells in fasting animals amounted to 23%, rising to 35% after histamine administration. At the same time a three-fold decrease in the secretory response (170 +/- 21 mkekv H+ over 3 h) was observed. Thiamine deficiency in the diet is also accompanied by inhibition of the transketolase activity in the gastric mucosa homogenate, which varied from 0.706 +/- 0.032 mcmole (control animals) to 0.344 +/- 0.034 mcmole of sedoheptuloso-7-phsophate/h per 1 mg protein (test animals). Potentialities of the pentose cycle participation in secretion are discussed.", "contents": "[Ultrastructure of the rat gastric mucosal parietal cells in thiamine deficiency]. The effect of the thiamine deficient diet on the ultrastructure and secretory response of parietal cells of the rat gastric mucosa was studied. The control group consisting of fasting animals showed 60% of the parietal cells to be in a presecretory state. Subcutaneous administration of histamine to the control animals was accompanied by secretion of 512 +/- 128 mkekv H+ over 3 hours, with only 18% of the parietal cells remaining not involved in the secretory process. In thiamine-free diets the number of secreting cells in fasting animals amounted to 23%, rising to 35% after histamine administration. At the same time a three-fold decrease in the secretory response (170 +/- 21 mkekv H+ over 3 h) was observed. Thiamine deficiency in the diet is also accompanied by inhibition of the transketolase activity in the gastric mucosa homogenate, which varied from 0.706 +/- 0.032 mcmole (control animals) to 0.344 +/- 0.034 mcmole of sedoheptuloso-7-phsophate/h per 1 mg protein (test animals). Potentialities of the pentose cycle participation in secretion are discussed."}
{"id": "PMID:483731", "title": "[Effect of ascorbic acid and B group vitamins on the course of subacute acrylonitrile poisoning in rats].", "content": "The effect of ascorbic acid and a mixture of pyridoxine and nicotinamide on the process of subacute poisoning with acrylonitrile (AN) in a concentration of 9.7 +/- 2.8 mg/m3 (corresponding to the threshold of a chronic action) was studied in rats. Administration of an additional mixture of vitamins had no significant effect on the course of subacute poisoning in rats. Changes in the physiological and biochemical parameters characteristic of the AN poisoning were noted in this group of animals at the end of the experiment. No significant changes were observed in the poisoning of rats after an additional administration of ascorbic acid either at the stage of the initial reactions, or at the end of the experiment. The results obtained indicate a strongly pronounced elevation of the adaptibility of the organism. This allows one to regard ascorbic acid as an alimentary factor of protective action in repeated subacute AN poisoning of the organism. Ascorbic acid can be recommended as a prophylactic agent for persons in continuous contact with AN under industrial conditions.", "contents": "[Effect of ascorbic acid and B group vitamins on the course of subacute acrylonitrile poisoning in rats]. The effect of ascorbic acid and a mixture of pyridoxine and nicotinamide on the process of subacute poisoning with acrylonitrile (AN) in a concentration of 9.7 +/- 2.8 mg/m3 (corresponding to the threshold of a chronic action) was studied in rats. Administration of an additional mixture of vitamins had no significant effect on the course of subacute poisoning in rats. Changes in the physiological and biochemical parameters characteristic of the AN poisoning were noted in this group of animals at the end of the experiment. No significant changes were observed in the poisoning of rats after an additional administration of ascorbic acid either at the stage of the initial reactions, or at the end of the experiment. The results obtained indicate a strongly pronounced elevation of the adaptibility of the organism. This allows one to regard ascorbic acid as an alimentary factor of protective action in repeated subacute AN poisoning of the organism. Ascorbic acid can be recommended as a prophylactic agent for persons in continuous contact with AN under industrial conditions."}
{"id": "PMID:483732", "title": "[Effect of the technological and culinary preparation on vitamin preservation in fish and in nonfishery products].", "content": "The content of vitamins A, E, C, B6, thiamine, riboflavin, niacin and folacin was studied comparatively in some fish species during their preservation as frozen, salt and smoked products. The thiamine content was found to decrease during preservation of frozen and salt fish, while in smoked fish it preserved better. The uncut salt and smoked fish showed an increased content of vitamin A in its edible part at the expense of redistribution together with fat from the internal organs. The boiling of fish and non-fishery products changed the content of some vitamins. Vitamin losses were due both to their destruction at boiling and passage into the broth.", "contents": "[Effect of the technological and culinary preparation on vitamin preservation in fish and in nonfishery products]. The content of vitamins A, E, C, B6, thiamine, riboflavin, niacin and folacin was studied comparatively in some fish species during their preservation as frozen, salt and smoked products. The thiamine content was found to decrease during preservation of frozen and salt fish, while in smoked fish it preserved better. The uncut salt and smoked fish showed an increased content of vitamin A in its edible part at the expense of redistribution together with fat from the internal organs. The boiling of fish and non-fishery products changed the content of some vitamins. Vitamin losses were due both to their destruction at boiling and passage into the broth."}
{"id": "PMID:483771", "title": "[Experience in selecting safe heat-sensitive influenza virus A recombinants].", "content": "Conditions for genetic recombination and laboratory testing of influenza A virus for rapid production of safe vaccine strains needed in human vaccination were developed. The best donors of attenuation were found to include limitingly attenuated outdated vaccine strains adapted to low temperature and having important differentiating features such as thermosensitivity and reduced optimal temperature of neuraminidase activity. Trials in volunteers of a large collection of recombinants produced by recombination of these donors with various virulent strains circulating in 1972--1976 (A/Victoria/72, A/Port Chalmers/73, and A/Virtoria/75) demonstrated complete safety for man of clones with different combinations of rct/40 and OTNA genetic markers.", "contents": "[Experience in selecting safe heat-sensitive influenza virus A recombinants]. Conditions for genetic recombination and laboratory testing of influenza A virus for rapid production of safe vaccine strains needed in human vaccination were developed. The best donors of attenuation were found to include limitingly attenuated outdated vaccine strains adapted to low temperature and having important differentiating features such as thermosensitivity and reduced optimal temperature of neuraminidase activity. Trials in volunteers of a large collection of recombinants produced by recombination of these donors with various virulent strains circulating in 1972--1976 (A/Victoria/72, A/Port Chalmers/73, and A/Virtoria/75) demonstrated complete safety for man of clones with different combinations of rct/40 and OTNA genetic markers."}
{"id": "PMID:483772", "title": "[Protective action of remantadine during an influenza outbreak caused by virus A (H1N1) in December 1977].", "content": "The protective effect of remantadin during an influenza outbreak in December 1977 caused by A (H1N1) virus was studied. A prophylactic administration of remantadin decreased influenza and ARD incidence by 1.5-fold. A more significant decrease of the incidence (2.5-fold) was observed among the subjects given a combination of remantadin and influenza vaccine (H3N2) a few days before the outbreak, which was apparently due to the interferon-inducing capacity of the vaccine.", "contents": "[Protective action of remantadine during an influenza outbreak caused by virus A (H1N1) in December 1977]. The protective effect of remantadin during an influenza outbreak in December 1977 caused by A (H1N1) virus was studied. A prophylactic administration of remantadin decreased influenza and ARD incidence by 1.5-fold. A more significant decrease of the incidence (2.5-fold) was observed among the subjects given a combination of remantadin and influenza vaccine (H3N2) a few days before the outbreak, which was apparently due to the interferon-inducing capacity of the vaccine."}
{"id": "PMID:483773", "title": "[Electron microscopic study of genome homology in 2 clones of the simian adenoviruses type 7].", "content": "Homology of the genomes of large-plaque (lp) and small-plaque (sp) clones of simian adenovirus type 7 (SV7) was studied experimentally. Guanidine chloride helped to isolate circular DNA molecules of identical length for both clones within the measurement error (1 = 11 +/- 0.2 micrometer). A heteroduplex SV7lp--SV7sp molecule was found to have two nonhomology sites 0.5 +/- 0.3 mu and 1.0 +/- 0.5 micrometer long located at a distance of 2 and 4 micrometer from one of the ends of the molecules, respectively.", "contents": "[Electron microscopic study of genome homology in 2 clones of the simian adenoviruses type 7]. Homology of the genomes of large-plaque (lp) and small-plaque (sp) clones of simian adenovirus type 7 (SV7) was studied experimentally. Guanidine chloride helped to isolate circular DNA molecules of identical length for both clones within the measurement error (1 = 11 +/- 0.2 micrometer). A heteroduplex SV7lp--SV7sp molecule was found to have two nonhomology sites 0.5 +/- 0.3 mu and 1.0 +/- 0.5 micrometer long located at a distance of 2 and 4 micrometer from one of the ends of the molecules, respectively."}
{"id": "PMID:483774", "title": "[Properties of a new, oncornavirus D-associated antigen].", "content": "Continuous cells of human origin, HEp-2, and mouse L929 cells taken 24 hours after infection with oncornavirus D were labeled for protein with a mixture of 14C-amino acids and 3H-glucosamine. Specific sera obtained from rabbits were used for testing the virus-specific new antigen in cell extracts and for immunoprecipitation of the new antigen. Electrophoresis in polyacryl amide gel showed the virusspecific new antigen, common for human HEp-2 cells and for oncornavirus-D-infected mouse L929 cells, to be a glycoprotein with a molecular weight of 98,000--100,000 daltons. By its molecular weight it differs from oncornavirus envelope glycoprotein whose molecular weight is 60,000--70,000 daltons. No similar protein was found in noninfected L929 cells.", "contents": "[Properties of a new, oncornavirus D-associated antigen]. Continuous cells of human origin, HEp-2, and mouse L929 cells taken 24 hours after infection with oncornavirus D were labeled for protein with a mixture of 14C-amino acids and 3H-glucosamine. Specific sera obtained from rabbits were used for testing the virus-specific new antigen in cell extracts and for immunoprecipitation of the new antigen. Electrophoresis in polyacryl amide gel showed the virusspecific new antigen, common for human HEp-2 cells and for oncornavirus-D-infected mouse L929 cells, to be a glycoprotein with a molecular weight of 98,000--100,000 daltons. By its molecular weight it differs from oncornavirus envelope glycoprotein whose molecular weight is 60,000--70,000 daltons. No similar protein was found in noninfected L929 cells."}
{"id": "PMID:483775", "title": "[Temperature-sensitive mutants of the Venezuelan equine encephalomyelitis virus studied in the complementation test].", "content": "Investigation of 15 ts mutants of Venezuelan equine encephalomyelitis (VEE) virus by complementation tests permitted to divide these mutants into 5 complementation groups and to distinguish unclassifiable mutants. Mutants of 2 groups had RNA+/- or RNA- phenotype, i. e. had defects in RNA synthesis. Mutants in the other 3 groups had the RNA+ phenotype, and the temperature-sensitive defect of these mutants was in later stages of virus reproduction.", "contents": "[Temperature-sensitive mutants of the Venezuelan equine encephalomyelitis virus studied in the complementation test]. Investigation of 15 ts mutants of Venezuelan equine encephalomyelitis (VEE) virus by complementation tests permitted to divide these mutants into 5 complementation groups and to distinguish unclassifiable mutants. Mutants of 2 groups had RNA+/- or RNA- phenotype, i. e. had defects in RNA synthesis. Mutants in the other 3 groups had the RNA+ phenotype, and the temperature-sensitive defect of these mutants was in later stages of virus reproduction."}
{"id": "PMID:483776", "title": "[Virus-specific RNA synthesis in a cell culture in acute and chronic infection with the tick-borne encephalitis virus].", "content": "Virus particles produced in acute and chronic infection of cell cultures with tick-borne encephalitis virus (TBE) and examined by centrifugation in sucrose density gradient had the buoyant density of 1.19 g/ml and sedimentation constant 290 S. Studies of the synthesis of virus-specific RNAs in the cytoplasm of TBE virus chronically infected cells revealed synthesis of all RNA classes typical of TBE virus reproduction in acutely infected cells. The difference was in the high percentage of polyadenylation of intracellular virion RNA in chronic infection (30%) as compared with polyadenylation of virion RNA in acute infection (8%). This fact may be one of the causes of a low infectious virus yield in chronically infected cultures where the level of virus-specific antigen synthesis is high.", "contents": "[Virus-specific RNA synthesis in a cell culture in acute and chronic infection with the tick-borne encephalitis virus]. Virus particles produced in acute and chronic infection of cell cultures with tick-borne encephalitis virus (TBE) and examined by centrifugation in sucrose density gradient had the buoyant density of 1.19 g/ml and sedimentation constant 290 S. Studies of the synthesis of virus-specific RNAs in the cytoplasm of TBE virus chronically infected cells revealed synthesis of all RNA classes typical of TBE virus reproduction in acutely infected cells. The difference was in the high percentage of polyadenylation of intracellular virion RNA in chronic infection (30%) as compared with polyadenylation of virion RNA in acute infection (8%). This fact may be one of the causes of a low infectious virus yield in chronically infected cultures where the level of virus-specific antigen synthesis is high."}
{"id": "PMID:483777", "title": "[Dynamic changes in the epidemiological patterns of measles in the People's Republic of Bulgaria after mass vaccination and revaccination].", "content": "The main epidemiological regularities of measles before vaccination (1960--1968) and at the stage of mass vaccination (1969--1977) were studied. The average annual incidence of measles has been reduced from 423.97 to 279.72 per 100,000, mortality from 0.56 to 0.10 0/0000, and lethality from 0.13% to 0.04%. The dynamical changes of postvaccination immunity were uneven: in 1969 vaccinations covered 30.67%, in 1970 28.82%, in 1971 64.09%, in 1972 53.38%, in 1973 59.61%, in 1974 67.53%, and in 1975 69.04%. The level of herd immunity in those years was lower than the optimal (45.35%, 43.97%, 67.71%, 68.13%, 74.65%, 83.25%, and 84.63%, respectively). In the first part of 1976 a general immunoprophylactic campaign was carried out including vaccination of the unvaccinated children born in 1963--1975 and revaccination of all those vaccinated one year before the campaign. Primary vaccination increased the coverage by 11.25%, and revaccination increased the portion of seropositive subjects by 5.2%. As a result, a sufficiently high level of general herd immunity has been achieved (1976--96.02%, 1977--96.10%) and the incidence decreased accordingly (1976--192.69 0/0000, 1977--9.17 0/0000).", "contents": "[Dynamic changes in the epidemiological patterns of measles in the People's Republic of Bulgaria after mass vaccination and revaccination]. The main epidemiological regularities of measles before vaccination (1960--1968) and at the stage of mass vaccination (1969--1977) were studied. The average annual incidence of measles has been reduced from 423.97 to 279.72 per 100,000, mortality from 0.56 to 0.10 0/0000, and lethality from 0.13% to 0.04%. The dynamical changes of postvaccination immunity were uneven: in 1969 vaccinations covered 30.67%, in 1970 28.82%, in 1971 64.09%, in 1972 53.38%, in 1973 59.61%, in 1974 67.53%, and in 1975 69.04%. The level of herd immunity in those years was lower than the optimal (45.35%, 43.97%, 67.71%, 68.13%, 74.65%, 83.25%, and 84.63%, respectively). In the first part of 1976 a general immunoprophylactic campaign was carried out including vaccination of the unvaccinated children born in 1963--1975 and revaccination of all those vaccinated one year before the campaign. Primary vaccination increased the coverage by 11.25%, and revaccination increased the portion of seropositive subjects by 5.2%. As a result, a sufficiently high level of general herd immunity has been achieved (1976--96.02%, 1977--96.10%) and the incidence decreased accordingly (1976--192.69 0/0000, 1977--9.17 0/0000)."}
{"id": "PMID:483781", "title": "[Effect of prolonged storage times on the infectivity of lyophilized arboviruses].", "content": "Lyophilized arboviruses of the following taxonomic groups: alfaviruses, flaviviruses, Bunyamwera supergroup viruses, as well as arenaviruses were used in the study. These viruses had been stored for a long time at --20 degree C. Determinations of the infectious titers in suckling mice showed that in a period of over 10 years (up to 21 years) no complete loss of infectivity occurred in any virus groups.", "contents": "[Effect of prolonged storage times on the infectivity of lyophilized arboviruses]. Lyophilized arboviruses of the following taxonomic groups: alfaviruses, flaviviruses, Bunyamwera supergroup viruses, as well as arenaviruses were used in the study. These viruses had been stored for a long time at --20 degree C. Determinations of the infectious titers in suckling mice showed that in a period of over 10 years (up to 21 years) no complete loss of infectivity occurred in any virus groups."}
{"id": "PMID:483782", "title": "[Use of the cation detergent, cetyltrimethylammonium bromide, in isolating the RNA of the influenza virus].", "content": "Isolation of virion RNA of influenza virus by the SDS-phenol method from subviral particles obtained from purified virions by treating them with a cation detergent, cetyl-methyl-ammonium bromide, increased RNA yields approximately 2-fold as compared with isolation of this RNA from whole virions.", "contents": "[Use of the cation detergent, cetyltrimethylammonium bromide, in isolating the RNA of the influenza virus]. Isolation of virion RNA of influenza virus by the SDS-phenol method from subviral particles obtained from purified virions by treating them with a cation detergent, cetyl-methyl-ammonium bromide, increased RNA yields approximately 2-fold as compared with isolation of this RNA from whole virions."}
{"id": "PMID:483778", "title": "[Oligopeptide mapping of the antigenic drift of influenza virus hemagglutinins].", "content": "Drift variants of influenza viruses of the groups Hsw1, H0 and H1 including the A/USSR/77 (H1N1) virus. Major subunits of Hsw1, H0, and H1 hemagglutinins having common oligopeptides differ considerably in other oligopeptides. Within the H1 antigen viruses there are differences between the viruses of the early (1947), middle (1950--1952), and late (1956) periods of circulation. The latter (1956) differ markedly from their predessessors. The A/USSR/77 virus is most close to A/Fort Warren/50 virus. Oligopeptide maps of minor hemagglutinin subunits of all the viruses under study differ little from each other. Some suggestions on the causes of the great variability of the HA1 chain and conservatism of the HA2 hemagglutinin chain are given.", "contents": "[Oligopeptide mapping of the antigenic drift of influenza virus hemagglutinins]. Drift variants of influenza viruses of the groups Hsw1, H0 and H1 including the A/USSR/77 (H1N1) virus. Major subunits of Hsw1, H0, and H1 hemagglutinins having common oligopeptides differ considerably in other oligopeptides. Within the H1 antigen viruses there are differences between the viruses of the early (1947), middle (1950--1952), and late (1956) periods of circulation. The latter (1956) differ markedly from their predessessors. The A/USSR/77 virus is most close to A/Fort Warren/50 virus. Oligopeptide maps of minor hemagglutinin subunits of all the viruses under study differ little from each other. Some suggestions on the causes of the great variability of the HA1 chain and conservatism of the HA2 hemagglutinin chain are given."}
{"id": "PMID:483790", "title": "Fiberoptic bronchoscopy in diagnosis of opportunistic lung infections: assessment of Sputa, Washings, Brushings and biopsy specimens.", "content": "Fiberoptic bronchoscopy (washings, brushings and biopsies) was done in 25 cases of proven opportunistic pulmonary infections in compromised hosts. Diagnostic yields of bronchoscopic procedures and expectorated sputum were compared. Sputum examination gave the lowest yield (14 percent). Bronchial washings and brushings were diagnostic in 30 percent and 38 percent of patients, respectively. On transbronchial biopsy of the lung (TBB) pathogens were identified in 75 percent of patients with Pneumocystis carinii infection and 67 percent of patients with other opportunistic infections. The overall TBB yield of 73 percent was superior (P<0.05) to that of either washings or brushings. The yield from combining washings and brushings was greater than from either procedure alone, but combination with TBB did not result in any significant improvement. TBB is recommended as a useful diagnostic procedure in patients with suspected opportunistic pulmonary infections. Morbidity was minimal with this procedure, and the need for thoracotomy was reduced when it was used.", "contents": "Fiberoptic bronchoscopy in diagnosis of opportunistic lung infections: assessment of Sputa, Washings, Brushings and biopsy specimens. Fiberoptic bronchoscopy (washings, brushings and biopsies) was done in 25 cases of proven opportunistic pulmonary infections in compromised hosts. Diagnostic yields of bronchoscopic procedures and expectorated sputum were compared. Sputum examination gave the lowest yield (14 percent). Bronchial washings and brushings were diagnostic in 30 percent and 38 percent of patients, respectively. On transbronchial biopsy of the lung (TBB) pathogens were identified in 75 percent of patients with Pneumocystis carinii infection and 67 percent of patients with other opportunistic infections. The overall TBB yield of 73 percent was superior (P<0.05) to that of either washings or brushings. The yield from combining washings and brushings was greater than from either procedure alone, but combination with TBB did not result in any significant improvement. TBB is recommended as a useful diagnostic procedure in patients with suspected opportunistic pulmonary infections. Morbidity was minimal with this procedure, and the need for thoracotomy was reduced when it was used."}
{"id": "PMID:483803", "title": "Health implications of increased coal use in the Western States.", "content": "The National Energy Plan proposed by President Carter provides for the rapid development of coal resources in the United States, particularly in the West. The potential consequences for health of this development were considered by the Advisory Committee on Health and Environmental Effects of Increased Coal Utilization, reporting to the Department of Energy. Their report recommended rigid adherence to pertinent existing regulations, improved environmental monitoring, expanded research in selected relevant topics and development of procedures for selecting the sites of new coal-fired power plants. Although the report was a major exercise in technology assessment, it is fundamentally a cautious document that proposes no new solutions or approaches. A review of occupational and community health problems associated with coal mining and coal utilization suggests that lessons from past experiences, especially in Appalachia, cannot be applied to the West uncritically. The two regions are fundamentally different in scale, topography and social development. In the West, future problems related to coal are likely to derive from unknown risks associated with coal processing technologies, land reclamation and water quality at the sites of power generation, and extensive social and demographic changes at centers of industrial activity that may have secondary effects on health. Additional considerations should supplement the recommendations of the Advisory Committee report.", "contents": "Health implications of increased coal use in the Western States. The National Energy Plan proposed by President Carter provides for the rapid development of coal resources in the United States, particularly in the West. The potential consequences for health of this development were considered by the Advisory Committee on Health and Environmental Effects of Increased Coal Utilization, reporting to the Department of Energy. Their report recommended rigid adherence to pertinent existing regulations, improved environmental monitoring, expanded research in selected relevant topics and development of procedures for selecting the sites of new coal-fired power plants. Although the report was a major exercise in technology assessment, it is fundamentally a cautious document that proposes no new solutions or approaches. A review of occupational and community health problems associated with coal mining and coal utilization suggests that lessons from past experiences, especially in Appalachia, cannot be applied to the West uncritically. The two regions are fundamentally different in scale, topography and social development. In the West, future problems related to coal are likely to derive from unknown risks associated with coal processing technologies, land reclamation and water quality at the sites of power generation, and extensive social and demographic changes at centers of industrial activity that may have secondary effects on health. Additional considerations should supplement the recommendations of the Advisory Committee report."}
{"id": "PMID:483804", "title": "Continuing education: a medical school responsibility?", "content": "A survey of medical school deans sheds some light on current attitudes and practices regarding continuing education. The role of medical schools as leaders in continuing medical education should be clarified.", "contents": "Continuing education: a medical school responsibility? A survey of medical school deans sheds some light on current attitudes and practices regarding continuing education. The role of medical schools as leaders in continuing medical education should be clarified."}
{"id": "PMID:483805", "title": "High altitude medical problems.", "content": "Increased travel to high altitude areas by mountaineers and nonclimbing tourists has emphasized the clinical problems associated with rapid ascent. Acute mountain sickness affects most sojourners at elevations above 10,000 feet. Symptoms are usually worse on the second or third day after arrival. Gradual ascent, spending one to three days at an intermediate altitude, and the use of acetazolamide (Diamox) will prevent or ameliorate symptoms in most instances. Serious and potentially fatal problems, such as high altitude pulmonary edema or cerebral edema, occur in approximately 0.5 percent to 1.0 percent of visitors to elevations above 10,000 feet-especially with heavy physical exertion on arrival, such as climbing or skiing. Early recognition, high flow oxygen therapy and prompt descent are crucially important in management. Our knowledge of the causes of these and other high altitude problems, such as retinal hemorrhage, systemic edema and pulmonary hypertension, is still incomplete. Even less is known of the effect of high altitudes on medical conditions common at sea level or on the action of commonly used drugs.", "contents": "High altitude medical problems. Increased travel to high altitude areas by mountaineers and nonclimbing tourists has emphasized the clinical problems associated with rapid ascent. Acute mountain sickness affects most sojourners at elevations above 10,000 feet. Symptoms are usually worse on the second or third day after arrival. Gradual ascent, spending one to three days at an intermediate altitude, and the use of acetazolamide (Diamox) will prevent or ameliorate symptoms in most instances. Serious and potentially fatal problems, such as high altitude pulmonary edema or cerebral edema, occur in approximately 0.5 percent to 1.0 percent of visitors to elevations above 10,000 feet-especially with heavy physical exertion on arrival, such as climbing or skiing. Early recognition, high flow oxygen therapy and prompt descent are crucially important in management. Our knowledge of the causes of these and other high altitude problems, such as retinal hemorrhage, systemic edema and pulmonary hypertension, is still incomplete. Even less is known of the effect of high altitudes on medical conditions common at sea level or on the action of commonly used drugs."}
{"id": "PMID:483824", "title": "[The biological classifications of malignant thyroid tumours (author's transl)].", "content": "Carcinomas of the thyroid gland display large pathological and clinical variation. Favourable prognostic factors accompary well-differentiated tumours, whereas poorly differentiated or anaplastic carcinomas tend to invade early and are usually rapidly fatal. Experience has demonstrated the difference in rate of growth, subsequent recurrence and late spread even in identically well-differentiated thyroid carcinomas. Biological difference must be the underlying reason for the clinical difference in behaviour of histologically similar cancers. This report demonstrates the correlation between in vivo radioactive iodine uptake, tumour staging and recurrence-free interval in 30 thyroid carcinomas. The uptake by well-differentiated carcinomas staged T2N0M0 is significantly higher than that by tumours staged T3N1M0, whereas the latter show a higher uptake than those tumours staged T3N1M0. A high tumour uptake rate is correlated with long recurrence-free interval (up to 24 months) within all groups of staging and vice versa.", "contents": "[The biological classifications of malignant thyroid tumours (author's transl)]. Carcinomas of the thyroid gland display large pathological and clinical variation. Favourable prognostic factors accompary well-differentiated tumours, whereas poorly differentiated or anaplastic carcinomas tend to invade early and are usually rapidly fatal. Experience has demonstrated the difference in rate of growth, subsequent recurrence and late spread even in identically well-differentiated thyroid carcinomas. Biological difference must be the underlying reason for the clinical difference in behaviour of histologically similar cancers. This report demonstrates the correlation between in vivo radioactive iodine uptake, tumour staging and recurrence-free interval in 30 thyroid carcinomas. The uptake by well-differentiated carcinomas staged T2N0M0 is significantly higher than that by tumours staged T3N1M0, whereas the latter show a higher uptake than those tumours staged T3N1M0. A high tumour uptake rate is correlated with long recurrence-free interval (up to 24 months) within all groups of staging and vice versa."}
{"id": "PMID:483825", "title": "[Extra-anatomical bypass grafting in high-risk patients (author's transl)].", "content": "Extra-anatomical grafting (axillo-femoral and femoro-femoral bypass) seems to present a possibility of saving the lower limbs from amputation in high-risk patients with severe decompensation from arterial occlusive diseases of the legs. A case description is given of 20 patients, in whom 21 bypass procedures were carried out. The results with axillo-femoral bypass proved satisfactory and this method of intervention can be recommended as an emergency operation in high-risk patients.", "contents": "[Extra-anatomical bypass grafting in high-risk patients (author's transl)]. Extra-anatomical grafting (axillo-femoral and femoro-femoral bypass) seems to present a possibility of saving the lower limbs from amputation in high-risk patients with severe decompensation from arterial occlusive diseases of the legs. A case description is given of 20 patients, in whom 21 bypass procedures were carried out. The results with axillo-femoral bypass proved satisfactory and this method of intervention can be recommended as an emergency operation in high-risk patients."}
{"id": "PMID:483826", "title": "[Endoscopic polypectomy in the colon (author's transl)].", "content": "A report is given on the endoscopic removal of 81 polyps of the colon by means of high frequency diathermy. This procedure proved to be without risk to the patient and has largely replaced surgical removal by laparotomy as the method of choice today. Total removal of the polyp with subsequent histological examination is naturally imperative to avoid unnecessary subsequent radical surgical procedures. Since most colonic carcinomas arise in the base of a polyp, polypectomy represents a true prophylactic measure and, in some cases, a therapeutic one.", "contents": "[Endoscopic polypectomy in the colon (author's transl)]. A report is given on the endoscopic removal of 81 polyps of the colon by means of high frequency diathermy. This procedure proved to be without risk to the patient and has largely replaced surgical removal by laparotomy as the method of choice today. Total removal of the polyp with subsequent histological examination is naturally imperative to avoid unnecessary subsequent radical surgical procedures. Since most colonic carcinomas arise in the base of a polyp, polypectomy represents a true prophylactic measure and, in some cases, a therapeutic one."}
{"id": "PMID:483827", "title": "[Pathological fractures of long medullary bones due to malignant tumours: indications for osteosynthesis (author's transl)].", "content": "51 cases of pathological fracture, mostly due to metastatic malignant tumours, are reviewed in respect to the methods and results of operative treatment. These results are compared with those of other authors. It is found that resection of the tumour and osteosynthesis combined with the use of bone cement is the best way of treatment to relieve the patients of pain, mobilize them as soon as possible and improve the quality of their short, remaining lifespan. Despite the positive approach towards operative treatment there are some limiting findings which indicate that an operation should not be undertaken in those patients whose estimated survival time is less than the time of recovery and mobilization.", "contents": "[Pathological fractures of long medullary bones due to malignant tumours: indications for osteosynthesis (author's transl)]. 51 cases of pathological fracture, mostly due to metastatic malignant tumours, are reviewed in respect to the methods and results of operative treatment. These results are compared with those of other authors. It is found that resection of the tumour and osteosynthesis combined with the use of bone cement is the best way of treatment to relieve the patients of pain, mobilize them as soon as possible and improve the quality of their short, remaining lifespan. Despite the positive approach towards operative treatment there are some limiting findings which indicate that an operation should not be undertaken in those patients whose estimated survival time is less than the time of recovery and mobilization."}
{"id": "PMID:483856", "title": "A multi-dose study on the human metabolism of amylobarbitone.", "content": "1. The metabolism of amylobarbitone has been investigated in two healthy male volunteers, and the urinary excretion of unchanged drug, and the products of omega and omega-1 oxidation, quantitatively determined for four days after each of three doses to each volunteer. 2. In contrast to observations with butobarbitone, amylobarbitone was shown to have little effect on the rate of its own metabolism. 3. The t 1/2 for urinary excretion of the parent drug was 16.3 h while that for the two metabolites was 22.2 h.", "contents": "A multi-dose study on the human metabolism of amylobarbitone. 1. The metabolism of amylobarbitone has been investigated in two healthy male volunteers, and the urinary excretion of unchanged drug, and the products of omega and omega-1 oxidation, quantitatively determined for four days after each of three doses to each volunteer. 2. In contrast to observations with butobarbitone, amylobarbitone was shown to have little effect on the rate of its own metabolism. 3. The t 1/2 for urinary excretion of the parent drug was 16.3 h while that for the two metabolites was 22.2 h."}
{"id": "PMID:483857", "title": "Some factors involved in the N-oxidation of 3-substituted pyridines by microsomal preparations in vitro.", "content": "1. Pyridine-N-oxides have been identified as metabolites in vitro of several 3-substituted pyridines. 2. Factors affecting the metabolism of these pyridines in vitro have been studied, and conditions which give the most metabolism have been established. 3. 'Pyridine-N-oxidase' activity resides mainly in the hepatic and pulmonary microsomal fractions. 4. A species difference was evident with 'pyridine-N-oxidase' activity decreasing in the order hamster, rabbit, mouse, guinea-pig and rat. 5. A sex difference in 'pyridine-N-oxidase' activity was also established in rats and mice. 6. The appropriate kinetic factors, Km and Vmax for the N-oxidation of pyridine, 3-methylpyridine and 3-chloropyridine are reported.", "contents": "Some factors involved in the N-oxidation of 3-substituted pyridines by microsomal preparations in vitro. 1. Pyridine-N-oxides have been identified as metabolites in vitro of several 3-substituted pyridines. 2. Factors affecting the metabolism of these pyridines in vitro have been studied, and conditions which give the most metabolism have been established. 3. 'Pyridine-N-oxidase' activity resides mainly in the hepatic and pulmonary microsomal fractions. 4. A species difference was evident with 'pyridine-N-oxidase' activity decreasing in the order hamster, rabbit, mouse, guinea-pig and rat. 5. A sex difference in 'pyridine-N-oxidase' activity was also established in rats and mice. 6. The appropriate kinetic factors, Km and Vmax for the N-oxidation of pyridine, 3-methylpyridine and 3-chloropyridine are reported."}
{"id": "PMID:483858", "title": "The effect of various potential inhibitors, activators and inducers on the N-oxidation of 3-substituted pyridines in vitro.", "content": "1. The N-oxidation of pyridine, 3-methylpyridine and 3-chloropyridine was inhibited by SKF525A and DPEA. The C-oxidation of 3-methylpyridine was also inhibited by these compounds. 2. The N-oxidation of these pyridines was also inhibited by various other nitrogenous substrates including n-octylamine. 3. Incubation in an atmosphere of carbon monoxide resulted in inhibition of both C- and N-oxidation of 3-methylpyridine. 4. Any treatment of microsomes which resulted in a reduction of cytochrome P-450 also produced a concomitant fall in N-oxidation of the pyridines. 5. Pretreatment of animals with phenobarbitone resulted in an increase in the N-oxidation of the pyridines. Pretreatment with 3-methylcholanthrene had no appreciable effect on the N-oxidation of the pyridines in vitro.", "contents": "The effect of various potential inhibitors, activators and inducers on the N-oxidation of 3-substituted pyridines in vitro. 1. The N-oxidation of pyridine, 3-methylpyridine and 3-chloropyridine was inhibited by SKF525A and DPEA. The C-oxidation of 3-methylpyridine was also inhibited by these compounds. 2. The N-oxidation of these pyridines was also inhibited by various other nitrogenous substrates including n-octylamine. 3. Incubation in an atmosphere of carbon monoxide resulted in inhibition of both C- and N-oxidation of 3-methylpyridine. 4. Any treatment of microsomes which resulted in a reduction of cytochrome P-450 also produced a concomitant fall in N-oxidation of the pyridines. 5. Pretreatment of animals with phenobarbitone resulted in an increase in the N-oxidation of the pyridines. Pretreatment with 3-methylcholanthrene had no appreciable effect on the N-oxidation of the pyridines in vitro."}
{"id": "PMID:483859", "title": "Metabolism of budralazine, a new antihypertensive agent, III.", "content": "1. [14C]Budralazine (I) administered orally to normotensive and spontaneously hypertensive rats showed no significant difference in metabolic fate between the two groups. 2. Peak plasma levels of 14C were 3.6 microgram equiv. of I per ml in both normotensive and hypertensive rats. Approx. 5% of total 14C in the plasma consisted of I within 4 h, but thereafter the parent drug was not detected. Approx. 15% of 14C in the plasma consisted of 1-hydrazinophthalazine within 16 h after the administration. 3. 45% and 37% of the dose of 14C was excreted into urine and faeces within 24 h, respectively. 4. A high retention of 14C in the aorta wall was found and the time course of 14C in the aorta wall was compatible with that of the reduction in blood pressure. The radioactive materials retained in the aorta wall were likely to be I or 1-hydrazinophthalazine, probably the latter, following macroautoradiography in hypertensive rats.", "contents": "Metabolism of budralazine, a new antihypertensive agent, III. 1. [14C]Budralazine (I) administered orally to normotensive and spontaneously hypertensive rats showed no significant difference in metabolic fate between the two groups. 2. Peak plasma levels of 14C were 3.6 microgram equiv. of I per ml in both normotensive and hypertensive rats. Approx. 5% of total 14C in the plasma consisted of I within 4 h, but thereafter the parent drug was not detected. Approx. 15% of 14C in the plasma consisted of 1-hydrazinophthalazine within 16 h after the administration. 3. 45% and 37% of the dose of 14C was excreted into urine and faeces within 24 h, respectively. 4. A high retention of 14C in the aorta wall was found and the time course of 14C in the aorta wall was compatible with that of the reduction in blood pressure. The radioactive materials retained in the aorta wall were likely to be I or 1-hydrazinophthalazine, probably the latter, following macroautoradiography in hypertensive rats."}
{"id": "PMID:483860", "title": "Biliary and urinary excretion of drug conjugates: effect of diuresis and choleresis on excretion of harmol sulphate and harmol glucuronide in the rat.", "content": "1. Harmol (7-hydroxy-1-methyl-9H-pyrido [3,4b] indole) is converted to harmol sulphate and harmol glucuronide in the rat in vivo. Harmol sulphate is excreted mainly in urine, while harmol glucuronide is excreted about equally in bile and urine, when harmol is given intravenously. 2. In rats with ligated kidneys, only choleresis produced by glycodehydrocholate and dehydrocholate caused enhanced biliary excretion of harmol sulphate; harmol glucuronide was unaffected. Several other bile salts had no effect. 3. Mannitol diuresis markedly increased urinary excretion of harmol sulphate, and decreased its biliary excretion. Harmol glucuronide was much less affected. 4. Nafenopin pretreatment increased liver weight and bile flow, and enhanced biliary excretion of harmol sulphate at the expense of its urinary excretion. 5. For harmol sulphate, urine and bile are compensatory pathways of elimination that can be influenced by urine and bile flow changes through diuresis and choleresis.", "contents": "Biliary and urinary excretion of drug conjugates: effect of diuresis and choleresis on excretion of harmol sulphate and harmol glucuronide in the rat. 1. Harmol (7-hydroxy-1-methyl-9H-pyrido [3,4b] indole) is converted to harmol sulphate and harmol glucuronide in the rat in vivo. Harmol sulphate is excreted mainly in urine, while harmol glucuronide is excreted about equally in bile and urine, when harmol is given intravenously. 2. In rats with ligated kidneys, only choleresis produced by glycodehydrocholate and dehydrocholate caused enhanced biliary excretion of harmol sulphate; harmol glucuronide was unaffected. Several other bile salts had no effect. 3. Mannitol diuresis markedly increased urinary excretion of harmol sulphate, and decreased its biliary excretion. Harmol glucuronide was much less affected. 4. Nafenopin pretreatment increased liver weight and bile flow, and enhanced biliary excretion of harmol sulphate at the expense of its urinary excretion. 5. For harmol sulphate, urine and bile are compensatory pathways of elimination that can be influenced by urine and bile flow changes through diuresis and choleresis."}
{"id": "PMID:483861", "title": "The metabolism of 3-amino-1-chloropropan-2-ol in relation to its antifertility activity in male rats.", "content": "1. The metabolism of 3-amino[36Cl]chloropropan-2-ol (III) was studied in male rats. Three urinary metabolites were isolated and identified as alpha-chlorohydrin (I), beta-chlorolactic acid (V) and oxalic acid (VI). Much of the administered aminochloropropanol was excreted unchanged in the urine; 63% within 72 h, 75% over 250 h. 2. Monoamine oxidase is capable of converting aminochloropropanol to beta-chlorolactaldehyde (IV) which, by processes of either reduction or oxidation, suggests that the metabolic pathway is IV leads to I and IV leads to V leads to VI. 3. As assessed by the diuretic activities of the isomers of aminochloropropanol, oxalate appears to be produced by the (+)-isomer but not by the (-)-isomer. A difference in metabolic rate or route of the isomers may account for their differing physiological activities. 4. (+)- and (-)-aminochloropropanol exhibited identical in vitro inhibitory activities on the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase, and were substrates for monoamine oxidase to equivalent extents.", "contents": "The metabolism of 3-amino-1-chloropropan-2-ol in relation to its antifertility activity in male rats. 1. The metabolism of 3-amino[36Cl]chloropropan-2-ol (III) was studied in male rats. Three urinary metabolites were isolated and identified as alpha-chlorohydrin (I), beta-chlorolactic acid (V) and oxalic acid (VI). Much of the administered aminochloropropanol was excreted unchanged in the urine; 63% within 72 h, 75% over 250 h. 2. Monoamine oxidase is capable of converting aminochloropropanol to beta-chlorolactaldehyde (IV) which, by processes of either reduction or oxidation, suggests that the metabolic pathway is IV leads to I and IV leads to V leads to VI. 3. As assessed by the diuretic activities of the isomers of aminochloropropanol, oxalate appears to be produced by the (+)-isomer but not by the (-)-isomer. A difference in metabolic rate or route of the isomers may account for their differing physiological activities. 4. (+)- and (-)-aminochloropropanol exhibited identical in vitro inhibitory activities on the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase, and were substrates for monoamine oxidase to equivalent extents."}
{"id": "PMID:483862", "title": "The metabolism of the anthelmintics clioxanide and resorantel and related compounds in vitro by Moniezia expansa, Ascaris suum and mouse- and sheep-liver enzymes.", "content": "1. Clioxanide and related compounds were deacetylated by the cestode Moniezia expansa, the nematode Ascaris suum, by enzymes prepared from these species and by mouse- and sheep-liver homogenates. Deacetylase activity was found in the cytosol of cestode proglottids throughout the strobila, and in the cytosol of nematode intestinal cells and reproductive tract. 2. The O-deacetylases from both helminths showed similar pH optima of about 7.0. Activity was enhanced by Ca2+ and low molecular weight thiols. Cu2+, Cd2+, Hg2+, Zn2+, and La2+ inhibited the deacetylation of clioxanide. 3. Resorantel and clioxanide were not hydrolysed at the amide bond by helminth or mammalian enzymes. 4. Resorantel was hydroxylated by mammalian microsomal enzymes, but helminths did not modify the molecule.", "contents": "The metabolism of the anthelmintics clioxanide and resorantel and related compounds in vitro by Moniezia expansa, Ascaris suum and mouse- and sheep-liver enzymes. 1. Clioxanide and related compounds were deacetylated by the cestode Moniezia expansa, the nematode Ascaris suum, by enzymes prepared from these species and by mouse- and sheep-liver homogenates. Deacetylase activity was found in the cytosol of cestode proglottids throughout the strobila, and in the cytosol of nematode intestinal cells and reproductive tract. 2. The O-deacetylases from both helminths showed similar pH optima of about 7.0. Activity was enhanced by Ca2+ and low molecular weight thiols. Cu2+, Cd2+, Hg2+, Zn2+, and La2+ inhibited the deacetylation of clioxanide. 3. Resorantel and clioxanide were not hydrolysed at the amide bond by helminth or mammalian enzymes. 4. Resorantel was hydroxylated by mammalian microsomal enzymes, but helminths did not modify the molecule."}
{"id": "PMID:483863", "title": "Structure and function of the conidiospore pigments of Penicillium cyclopium.", "content": "The cell wall of mature, green condiospores of Penicillium cyclopium Westling contains at least two pigments: A green chromoprotein which is extractable by means of formic acid or liquified phenol and a black insoluble pigment. Both fractions after long term treatment with boiling conc. HCl leave black amorphous residues which, due to their chemical and physico-chemical properties, belong to the group of melanins. The chemical structure of these melanins is still unidentified. No degradation products typical for indol-type or catechol-type melanins have so far been detected. During spore maturation parallel to an increase of pigmentation (determined by remission), the melanin residue left after acid hydrolysis of spores increases. The mature, dark green spores of the wild type strain contain about 40% melanin, the yellow-green spores of the mutant aux-glu 1 about 36%. The unpigmented spores of mutant res-eth 1 possess a melanin content of only about 5%. This value is nearly the same as that found in hyphae, which in all strains are yellowish-brown. The heavily pigmented condia of the wild type strain are about 100-times less sensitive to UV-radiation compared with the unpigmented spores of the mutant res-eth 1. The reduced sensitivity indicates that, as with other microorganisms, the conidia pigments of P. cyclopium are protective components of the spores.", "contents": "Structure and function of the conidiospore pigments of Penicillium cyclopium. The cell wall of mature, green condiospores of Penicillium cyclopium Westling contains at least two pigments: A green chromoprotein which is extractable by means of formic acid or liquified phenol and a black insoluble pigment. Both fractions after long term treatment with boiling conc. HCl leave black amorphous residues which, due to their chemical and physico-chemical properties, belong to the group of melanins. The chemical structure of these melanins is still unidentified. No degradation products typical for indol-type or catechol-type melanins have so far been detected. During spore maturation parallel to an increase of pigmentation (determined by remission), the melanin residue left after acid hydrolysis of spores increases. The mature, dark green spores of the wild type strain contain about 40% melanin, the yellow-green spores of the mutant aux-glu 1 about 36%. The unpigmented spores of mutant res-eth 1 possess a melanin content of only about 5%. This value is nearly the same as that found in hyphae, which in all strains are yellowish-brown. The heavily pigmented condia of the wild type strain are about 100-times less sensitive to UV-radiation compared with the unpigmented spores of the mutant res-eth 1. The reduced sensitivity indicates that, as with other microorganisms, the conidia pigments of P. cyclopium are protective components of the spores."}
{"id": "PMID:483864", "title": "[Electron microscopic studies of the insertion of pili in the newly isolated hydrogen-oxidizing bacterium 2K1].", "content": "Cells of the hydrogen-oxidizing bacterium 2K1 exhibited numerous pili of varying length, with a diameter of 7--8 nm as measured from negatively stained samples. At the basis of each pilus, a cell wall differentiation was observed appearing, in face-on-view, as a ring-like structure made up of subunits, and in side-on view as a hollow cylinder penetrating through the cell wall. It is assumed that these ring-like structures are in direct relation with the presence of the pili. As the number of ring-like structures per cell was higher than the number of pili per cell, rings without associated pili might either be sites of polymerizing pili, or \"dead\" pilus channel complexes out of function due to pilus break-down.", "contents": "[Electron microscopic studies of the insertion of pili in the newly isolated hydrogen-oxidizing bacterium 2K1]. Cells of the hydrogen-oxidizing bacterium 2K1 exhibited numerous pili of varying length, with a diameter of 7--8 nm as measured from negatively stained samples. At the basis of each pilus, a cell wall differentiation was observed appearing, in face-on-view, as a ring-like structure made up of subunits, and in side-on view as a hollow cylinder penetrating through the cell wall. It is assumed that these ring-like structures are in direct relation with the presence of the pili. As the number of ring-like structures per cell was higher than the number of pili per cell, rings without associated pili might either be sites of polymerizing pili, or \"dead\" pilus channel complexes out of function due to pilus break-down."}
{"id": "PMID:483868", "title": "[Effect of polyene antibiotics and their perhydrovderivatives on intact cells and protoplasts of yeast Candida guilliermondii].", "content": "Perhydroderivatives of polyene antibiotics have a much lower activity against eukaryotic cells than the polyene antibiotics itself. Bacterial cells are normally resistant against most polyene antibiotics and their perhydroderivatives. In earlier experiments with wall less L-form cells of Escherichia coli we have shown that the bacterial cell wall may be responsible for the resistance of the intact bacterial cells against polyene antibiotics and their perhydroderivatives by masking internal target sites. In the present paper we studied the effect of polyene antibiotics and their perhydroderivatives on intact cells and protoplasts of Candida guilliermondii. Our experiments have shown that most of the perhydroderivatives studied had a lower activity against intact cells as well as protoplasts than the corresponding polyene antibiotics. This means that in the case of eukaryotic cells the cell wall as a penetration barrier cannot mainly be responsible for the low activity of perhydroderivatives. The results are compared with those obtained previously with intact cells and protoplast type L-form cells of E. coli.", "contents": "[Effect of polyene antibiotics and their perhydrovderivatives on intact cells and protoplasts of yeast Candida guilliermondii]. Perhydroderivatives of polyene antibiotics have a much lower activity against eukaryotic cells than the polyene antibiotics itself. Bacterial cells are normally resistant against most polyene antibiotics and their perhydroderivatives. In earlier experiments with wall less L-form cells of Escherichia coli we have shown that the bacterial cell wall may be responsible for the resistance of the intact bacterial cells against polyene antibiotics and their perhydroderivatives by masking internal target sites. In the present paper we studied the effect of polyene antibiotics and their perhydroderivatives on intact cells and protoplasts of Candida guilliermondii. Our experiments have shown that most of the perhydroderivatives studied had a lower activity against intact cells as well as protoplasts than the corresponding polyene antibiotics. This means that in the case of eukaryotic cells the cell wall as a penetration barrier cannot mainly be responsible for the low activity of perhydroderivatives. The results are compared with those obtained previously with intact cells and protoplast type L-form cells of E. coli."}
{"id": "PMID:483916", "title": "[Inhibitors of platelet aggregation in the therapy of arteriosclerosis obliterans].", "content": "The central role of the thrombocytes in the initial atherogenesis and the secondary formation of the thrombus on sclerotically changed walls of the vessels is the basis of all experiments to perform a primary or secondary prevention of the atherosclerosis with thrombocyte aggregation inhibitors. Of the numerous thrombocyte-inhibiting substances acetyl salicylic acid, dipyridamol and sulfinpyrazone proved most suitable for clinical purposes. Apparantly for methodical reasons experiments of a primary prevention of the atherosclerosis with aggregation inhibitors have hitherto not be performed. On the other hand, in numerous quantitatively very different studies on patients with manifest cerebral, coronary and peripheral arteriosclerosis the influencibility of the course of the disease was tested by thrombocyte inhibitors. Since correct prospective studies are connected with enormous organisational expenditure and must extend for a longer period many extensive examinations have not yet finished. In the present survey the hitherto existing reports from literature are critically summarized, taking into particular consideration the per ipheral angioorganopathies and first informations on an own study concerning the prophylaxis of the diabetic angiopathy with Micristin are given. The present state of knowledge allows the conclusion that the well founded theoretical concept is apparantly confirmed by practice. However, the successes observed could be statistically ascertained only in individual cases, so that at present a final estimation of the prophylactic value of these preparations is not yet possible.", "contents": "[Inhibitors of platelet aggregation in the therapy of arteriosclerosis obliterans]. The central role of the thrombocytes in the initial atherogenesis and the secondary formation of the thrombus on sclerotically changed walls of the vessels is the basis of all experiments to perform a primary or secondary prevention of the atherosclerosis with thrombocyte aggregation inhibitors. Of the numerous thrombocyte-inhibiting substances acetyl salicylic acid, dipyridamol and sulfinpyrazone proved most suitable for clinical purposes. Apparantly for methodical reasons experiments of a primary prevention of the atherosclerosis with aggregation inhibitors have hitherto not be performed. On the other hand, in numerous quantitatively very different studies on patients with manifest cerebral, coronary and peripheral arteriosclerosis the influencibility of the course of the disease was tested by thrombocyte inhibitors. Since correct prospective studies are connected with enormous organisational expenditure and must extend for a longer period many extensive examinations have not yet finished. In the present survey the hitherto existing reports from literature are critically summarized, taking into particular consideration the per ipheral angioorganopathies and first informations on an own study concerning the prophylaxis of the diabetic angiopathy with Micristin are given. The present state of knowledge allows the conclusion that the well founded theoretical concept is apparantly confirmed by practice. However, the successes observed could be statistically ascertained only in individual cases, so that at present a final estimation of the prophylactic value of these preparations is not yet possible."}
{"id": "PMID:483917", "title": "[Vascular complications in primary hyperlipoproteinemias (HLP) with special reference to changes in the eye fundus].", "content": "The distribution of the types of hyperlipoproteinaemias revealed 2 peaks of frequency, i. e. in the types IIa and IIb corresponding 41% and in the types IV and V corresponding 55%. Weight indices and disturbed glucose tolerance are in the types III-V significantly higher than in the types IIa and IIb. The risk factor hypertension was registered in 34% in the investigation material. The highest prevalence rates for myocardial infarctions were in the patients of the types IIb and III, for PAD in the type III. The addition of hypertension and diabetes mellitus to hyperlipoproteinaemia reveals an exacerbation of the vascular risk. Changes of the fundus oculi, which correspond to the picture of a fundus hypertonicus cause to think of the presence of a hyperlipoproteinaemia in all patients with normotension.", "contents": "[Vascular complications in primary hyperlipoproteinemias (HLP) with special reference to changes in the eye fundus]. The distribution of the types of hyperlipoproteinaemias revealed 2 peaks of frequency, i. e. in the types IIa and IIb corresponding 41% and in the types IV and V corresponding 55%. Weight indices and disturbed glucose tolerance are in the types III-V significantly higher than in the types IIa and IIb. The risk factor hypertension was registered in 34% in the investigation material. The highest prevalence rates for myocardial infarctions were in the patients of the types IIb and III, for PAD in the type III. The addition of hypertension and diabetes mellitus to hyperlipoproteinaemia reveals an exacerbation of the vascular risk. Changes of the fundus oculi, which correspond to the picture of a fundus hypertonicus cause to think of the presence of a hyperlipoproteinaemia in all patients with normotension."}
{"id": "PMID:483918", "title": "[Autoantibodies in arteriosclerosi and endangiitis obliterans].", "content": "10 of 14 patients with obliterating endangiitis and 2 of 17 patients with obliterating arteriosclerosis had in the serum immunoflorescence-optically provable low titre antibodies against different constituents of smooth musculature (once cross-reacting with transverse and cross striation of the myocardium). Further antibodiies against brush border of proximal renal tubuli, parietal cells, cardiac sarcolemma, cytoplasm of the liver and distal renal tubuli were found. Antibodies against nuclear material, skeletal musculature, further typical antibodies against mitochondria, ribosomes and membranes of the endoplasmatic reticulum as well as so-called pemphigus and pemphigoid antibodies could not be proved. The proof of low titre antibodies against constituents of smooth musculature is in this case regarded as an unspecific marker of an (often) viral inflammation.", "contents": "[Autoantibodies in arteriosclerosi and endangiitis obliterans]. 10 of 14 patients with obliterating endangiitis and 2 of 17 patients with obliterating arteriosclerosis had in the serum immunoflorescence-optically provable low titre antibodies against different constituents of smooth musculature (once cross-reacting with transverse and cross striation of the myocardium). Further antibodiies against brush border of proximal renal tubuli, parietal cells, cardiac sarcolemma, cytoplasm of the liver and distal renal tubuli were found. Antibodies against nuclear material, skeletal musculature, further typical antibodies against mitochondria, ribosomes and membranes of the endoplasmatic reticulum as well as so-called pemphigus and pemphigoid antibodies could not be proved. The proof of low titre antibodies against constituents of smooth musculature is in this case regarded as an unspecific marker of an (often) viral inflammation."}
{"id": "PMID:483919", "title": "[The value of thoracoscopic sympathectomy in the treatment of Raynaud's syndrome].", "content": "On the basis of a follow-up period lasting at least 6 years concerning 23 patients, in whom on account of a Raynaud-syndrome a thoracoscopic sympathicotomy in the region Th 2-5 was carried out in most cases bilaterally in two sessions, in comparison with the sympathicectomy performed in major surgery can be established:no mortality, no serious complications, duration of stay in hospital 5-7 days, no absolute healing. In a follow-up period of the same length in an approach of major surgery absolute healings are possible, but only in 35%. This gain is loaded by a longer stay in hospital of at least 10 days and a mortality of 4.3% as well as by a complication rate of 6-41%, to which in 8-13% of the cases a Horner-syndrome comes. Therefore, before an approach in major surgery a thoracoscopic intervention is to be tried. The thoracoscopic operators should strive for a sympathectomy with destroy of the ganglia Th 2 and 3 instead for a sympathicotomy.", "contents": "[The value of thoracoscopic sympathectomy in the treatment of Raynaud's syndrome]. On the basis of a follow-up period lasting at least 6 years concerning 23 patients, in whom on account of a Raynaud-syndrome a thoracoscopic sympathicotomy in the region Th 2-5 was carried out in most cases bilaterally in two sessions, in comparison with the sympathicectomy performed in major surgery can be established:no mortality, no serious complications, duration of stay in hospital 5-7 days, no absolute healing. In a follow-up period of the same length in an approach of major surgery absolute healings are possible, but only in 35%. This gain is loaded by a longer stay in hospital of at least 10 days and a mortality of 4.3% as well as by a complication rate of 6-41%, to which in 8-13% of the cases a Horner-syndrome comes. Therefore, before an approach in major surgery a thoracoscopic intervention is to be tried. The thoracoscopic operators should strive for a sympathectomy with destroy of the ganglia Th 2 and 3 instead for a sympathicotomy."}
{"id": "PMID:483921", "title": "[Morphology of acute and chronic pancreatitis].", "content": "The pathological anatomy of pancreatitis is treated, based on a classification in acute haemorrhagic necrotizing (tryptic), acute serous (interstitial), acute purulent, chronic sclerosing (primary) and chronic relapsing (tryptic) pancreatitis, in which case is referred to the problems of such classifications. Among the individual forms etiologic and pathogenetic points of view are briefly discussed, among others also questions of the autoimmune pancreatitis and of the key positions of the acinus cell in the development of the necrotizing pancreatitis, taking into consideration experimental result. Further paragraphs deal with the special forms as well as with the course and the complications of pancreatitis, taking above all into consideration morphologic aspects and actual problems.", "contents": "[Morphology of acute and chronic pancreatitis]. The pathological anatomy of pancreatitis is treated, based on a classification in acute haemorrhagic necrotizing (tryptic), acute serous (interstitial), acute purulent, chronic sclerosing (primary) and chronic relapsing (tryptic) pancreatitis, in which case is referred to the problems of such classifications. Among the individual forms etiologic and pathogenetic points of view are briefly discussed, among others also questions of the autoimmune pancreatitis and of the key positions of the acinus cell in the development of the necrotizing pancreatitis, taking into consideration experimental result. Further paragraphs deal with the special forms as well as with the course and the complications of pancreatitis, taking above all into consideration morphologic aspects and actual problems."}
{"id": "PMID:483922", "title": "[The contribution of ERCP to the diagnosis of chronic pancreatitis].", "content": "A survey is given of the present state of the ERCP in the diagnostics of the chronic pancreatitis. The ERCP is not suited for the proof of early changes in the chronic pancreatitis; it does not allow an exclusion diagnosis. The value of the ERCP in the chronic pancreatitis consists in the fact to render the indication to operation by the proof changes which should be operated on, or to precise it, respectively, and to prevent unnecessary test laparotomies. For this reason the ERCP is indicated above all in the ascertained chronically relapsing pancreatitis and in the persistence of symptoms after an acute inflammatory attack. The most comprehensive information about the morphologic state of the pancreas is got by the combination of the ERCP with methods which reflect the size and the external form of the organ and give evidence concerning the structural changes (sonography, computer tomography).", "contents": "[The contribution of ERCP to the diagnosis of chronic pancreatitis]. A survey is given of the present state of the ERCP in the diagnostics of the chronic pancreatitis. The ERCP is not suited for the proof of early changes in the chronic pancreatitis; it does not allow an exclusion diagnosis. The value of the ERCP in the chronic pancreatitis consists in the fact to render the indication to operation by the proof changes which should be operated on, or to precise it, respectively, and to prevent unnecessary test laparotomies. For this reason the ERCP is indicated above all in the ascertained chronically relapsing pancreatitis and in the persistence of symptoms after an acute inflammatory attack. The most comprehensive information about the morphologic state of the pancreas is got by the combination of the ERCP with methods which reflect the size and the external form of the organ and give evidence concerning the structural changes (sonography, computer tomography)."}
{"id": "PMID:483923", "title": "[Surgical treatment of acute and chronic pancreatitis].", "content": "The acute haemorrhagically necrotizing pancreatitis demands, as a rule, an operative sanation of the local complications. The partial or total pancreatectomy during the first days for preventing the late local complications is loaded with an operation mortality from 35 to 60%. If the early operation is limited to the removal of recognizable and demarkated necroses, the mortality decreases to 30 or 35%. In principal preference of a retarded date of operation and in consequent sanation of local changes, if necessary in repeated interventions, the mortality is between 20 and 33%. The surgical treatment of the chronic pancreatitis shall concerning indication to operation, tactics and kind of intervention always take into consideration an existing abuse of alcohol. Long-term controls revealed that the most essential life-limiting factor is a further existing alcoholism. The more parenchyma is removed in operation the higher is the late mortality.", "contents": "[Surgical treatment of acute and chronic pancreatitis]. The acute haemorrhagically necrotizing pancreatitis demands, as a rule, an operative sanation of the local complications. The partial or total pancreatectomy during the first days for preventing the late local complications is loaded with an operation mortality from 35 to 60%. If the early operation is limited to the removal of recognizable and demarkated necroses, the mortality decreases to 30 or 35%. In principal preference of a retarded date of operation and in consequent sanation of local changes, if necessary in repeated interventions, the mortality is between 20 and 33%. The surgical treatment of the chronic pancreatitis shall concerning indication to operation, tactics and kind of intervention always take into consideration an existing abuse of alcohol. Long-term controls revealed that the most essential life-limiting factor is a further existing alcoholism. The more parenchyma is removed in operation the higher is the late mortality."}
{"id": "PMID:483924", "title": "[Hepatological aspects of surgical interventions in the stomach].", "content": "By means of quantitative-biochemical and histomorphological methods after vagotomies changes of the liver could be proved to a smaller extent than after resections of the stomach (Billroth II). Among the bionomic operation methods the supra-selective proximal vagotomy without pylorplastics is superior to other methods under the aspect of the change of the liver.", "contents": "[Hepatological aspects of surgical interventions in the stomach]. By means of quantitative-biochemical and histomorphological methods after vagotomies changes of the liver could be proved to a smaller extent than after resections of the stomach (Billroth II). Among the bionomic operation methods the supra-selective proximal vagotomy without pylorplastics is superior to other methods under the aspect of the change of the liver."}
{"id": "PMID:483925", "title": "[Early diagnosis of diabetes mellitus--studies on the effectiveness and value of diabetes screening actions].", "content": "Actions to found out cases of diabetes, which exclusively worked with a proof of glucosuria using the biophan-test stripe, were always loaded by a high percentage of falsely pathologic, but also by a not insignificant number of falsely negative investigation results. -- Therefore a multiphasic screening was carried out in 5,424 persons at the age of 20 and more years, which tested the problems mentioned and judged the possibilities of an improved diagnosis.", "contents": "[Early diagnosis of diabetes mellitus--studies on the effectiveness and value of diabetes screening actions]. Actions to found out cases of diabetes, which exclusively worked with a proof of glucosuria using the biophan-test stripe, were always loaded by a high percentage of falsely pathologic, but also by a not insignificant number of falsely negative investigation results. -- Therefore a multiphasic screening was carried out in 5,424 persons at the age of 20 and more years, which tested the problems mentioned and judged the possibilities of an improved diagnosis."}
{"id": "PMID:483926", "title": "[Diagnosis of malignant non-Hodgkin lymphoma on the basis of the Kiel-classification].", "content": "In short form a survey is given concerning the division of the malignant non-Hodgkin-lymphoma according to the classification of Kiel. The subdivision in stages and a proposal for a comprehensive clinical and laboratory investigation programme complete the compilation concerning the diagnostics of the malignant non-Hodgkin-lymphomas.", "contents": "[Diagnosis of malignant non-Hodgkin lymphoma on the basis of the Kiel-classification]. In short form a survey is given concerning the division of the malignant non-Hodgkin-lymphoma according to the classification of Kiel. The subdivision in stages and a proposal for a comprehensive clinical and laboratory investigation programme complete the compilation concerning the diagnostics of the malignant non-Hodgkin-lymphomas."}
{"id": "PMID:483927", "title": "[Experiences with fine needle aspiration biopsy of the thyroid gland].", "content": "It is reported on 155 fine needle aspiration biopsies of the thyroid gland which above all were performed in cold nodes. In 81.9% of the cases unconspicuous cell pictures or cystico-regressive changes were present. Of the 17 findings suspicuous of malignoma (11%) hitherto 2 could histologically be diagnosed as carcinomas and 3 as adenomas with considerable regressive changes. The additionally established frequency of nodular strumas and cold nodes in 547 scintigraphically examined patients had the results of 54% nodular strumas and 48.4% of cold nodes in the total material. In 90.2% the nodular strumas revealed cold nodes. The rate of malignomas of all preparations of operations of the thyroid gland was altogether at 2.9% subtracting the secondary malignant processes 2.4% were found. These findings as well as the positive data from literature give the fine needle aspiration biopsy an important role in supplementing the morphologic diagnostics and the early recognition of malignomas of the thyroid gland.", "contents": "[Experiences with fine needle aspiration biopsy of the thyroid gland]. It is reported on 155 fine needle aspiration biopsies of the thyroid gland which above all were performed in cold nodes. In 81.9% of the cases unconspicuous cell pictures or cystico-regressive changes were present. Of the 17 findings suspicuous of malignoma (11%) hitherto 2 could histologically be diagnosed as carcinomas and 3 as adenomas with considerable regressive changes. The additionally established frequency of nodular strumas and cold nodes in 547 scintigraphically examined patients had the results of 54% nodular strumas and 48.4% of cold nodes in the total material. In 90.2% the nodular strumas revealed cold nodes. The rate of malignomas of all preparations of operations of the thyroid gland was altogether at 2.9% subtracting the secondary malignant processes 2.4% were found. These findings as well as the positive data from literature give the fine needle aspiration biopsy an important role in supplementing the morphologic diagnostics and the early recognition of malignomas of the thyroid gland."}
{"id": "PMID:483928", "title": "[Eye fundus pathology in chronic terminal kidney failure].", "content": "On 75 patients with chronic renal insufficiency the relations between renal hypertension due to volume expansion and findings of the fundus of the eye were investigated. In compensated renal insufficiency in 20 of 23 patients a hypertension appeared, hypertensively conditioned changes of the fundus were observed only in about one third of the cases. When the functional disturbances progressed into the stage of the chronic terminal renale insufficiency on the other hand in 27 of 38 patients retina findings conditioned by hypertension were stated. Among 38 haemodialysis patients by regulation of the fluid balance 11 times a normal fundus and 15 times an improvement of the findings of the fundus of the eye could be revealed. In 13 patients the findings of the fundus generally improved after successful renal transplantation. Also our examinations call the usual stage subdivisions after Thiel and Keith and co-workers, respectively, in question.", "contents": "[Eye fundus pathology in chronic terminal kidney failure]. On 75 patients with chronic renal insufficiency the relations between renal hypertension due to volume expansion and findings of the fundus of the eye were investigated. In compensated renal insufficiency in 20 of 23 patients a hypertension appeared, hypertensively conditioned changes of the fundus were observed only in about one third of the cases. When the functional disturbances progressed into the stage of the chronic terminal renale insufficiency on the other hand in 27 of 38 patients retina findings conditioned by hypertension were stated. Among 38 haemodialysis patients by regulation of the fluid balance 11 times a normal fundus and 15 times an improvement of the findings of the fundus of the eye could be revealed. In 13 patients the findings of the fundus generally improved after successful renal transplantation. Also our examinations call the usual stage subdivisions after Thiel and Keith and co-workers, respectively, in question."}
{"id": "PMID:483929", "title": "[Occupational respiratory tract allergies].", "content": "Professionally conditioned allergoses of the respiratory tract (BK 41) can occur in all industrial regions. The allergoses conditioned by organic dusts of vegetable or animal origin (regions food production, plant production and animal keeping) predominate. Since 1976 the BK 41 has been on the 10th rank of alases, 1977), above all conditioned by an improved establishment. Among the diagnoses bronchial asthma is in the first place. The high proportion of allergoses by cereal constituents is to be led back to the, as a rule, unproblematic allergologic clarification diagnostics. One may conclude that difficulties of the recognition in other allergens condition a considerable dark number, particularly in chemical working materials. The allergoses by cereal constituents are followed by the constituents of the animal epidermis (above all hair of laboratory animals). The remainder -- without any importance of the succession -- belongs to moulds, mites, plant pollen, parasubstituted aromates, chrome and rare allergens. Apart from the slight number of alveolitis diseases the allergoses conditioned by organic dusts belong to the type of early reaction (characteristic representatives: baker's asthma). Problematical are the allergoses of the type of the late reaction. Chemical substances of the hapten type are above all in question (characteristic representative: chrome asthma). Here is most cases expressed irritation effects are present so that this pathogenetic factor is frequently determining without proving any immune-specific effects. The report is based on forgiven criteria of decision.", "contents": "[Occupational respiratory tract allergies]. Professionally conditioned allergoses of the respiratory tract (BK 41) can occur in all industrial regions. The allergoses conditioned by organic dusts of vegetable or animal origin (regions food production, plant production and animal keeping) predominate. Since 1976 the BK 41 has been on the 10th rank of alases, 1977), above all conditioned by an improved establishment. Among the diagnoses bronchial asthma is in the first place. The high proportion of allergoses by cereal constituents is to be led back to the, as a rule, unproblematic allergologic clarification diagnostics. One may conclude that difficulties of the recognition in other allergens condition a considerable dark number, particularly in chemical working materials. The allergoses by cereal constituents are followed by the constituents of the animal epidermis (above all hair of laboratory animals). The remainder -- without any importance of the succession -- belongs to moulds, mites, plant pollen, parasubstituted aromates, chrome and rare allergens. Apart from the slight number of alveolitis diseases the allergoses conditioned by organic dusts belong to the type of early reaction (characteristic representatives: baker's asthma). Problematical are the allergoses of the type of the late reaction. Chemical substances of the hapten type are above all in question (characteristic representative: chrome asthma). Here is most cases expressed irritation effects are present so that this pathogenetic factor is frequently determining without proving any immune-specific effects. The report is based on forgiven criteria of decision."}
{"id": "PMID:483931", "title": "[Diagnostic problems of tuberculosis in old age].", "content": "Among the present epidemiologic conditions postprimary tuberculoses gain importance due to the exazerbation of latent foci. The particularly concerns the generalised senile tuberculosis. The clinical picture of a generalisation tuberculosis deviates in comparison with the course which was formerly regarded as typical. It shows a protracted, at first undramatic phase with slowly progressing development, appears intermittently, is characterized by rich, variants with regard to form, course and localisation, withdraws from radiological demonstration for a long time and thus progresses occult in general. The 35 cases should remind of diagnostic basic patterns and should take into consideration the peculiarities of exacerbated generalised tuberculoses at old age. The observation of symptomatology and paraclinical findings, bioptic examinations of the liver, bacteriologic or histologic findings of the adequate substrate, the exclusion of other organic diseases and an experiment of an antituberculotic treatment when tuberculosis is supposed may become successful measures of making the diagnosis. Even nowadays tuberculosis must be included in the differential diagnosis of several organic diseases.", "contents": "[Diagnostic problems of tuberculosis in old age]. Among the present epidemiologic conditions postprimary tuberculoses gain importance due to the exazerbation of latent foci. The particularly concerns the generalised senile tuberculosis. The clinical picture of a generalisation tuberculosis deviates in comparison with the course which was formerly regarded as typical. It shows a protracted, at first undramatic phase with slowly progressing development, appears intermittently, is characterized by rich, variants with regard to form, course and localisation, withdraws from radiological demonstration for a long time and thus progresses occult in general. The 35 cases should remind of diagnostic basic patterns and should take into consideration the peculiarities of exacerbated generalised tuberculoses at old age. The observation of symptomatology and paraclinical findings, bioptic examinations of the liver, bacteriologic or histologic findings of the adequate substrate, the exclusion of other organic diseases and an experiment of an antituberculotic treatment when tuberculosis is supposed may become successful measures of making the diagnosis. Even nowadays tuberculosis must be included in the differential diagnosis of several organic diseases."}
{"id": "PMID:483932", "title": "[Pleural asbestosis in dock workers].", "content": "During the last three years 29 patients with asbestosis of the pleura were examined. 21 of them were exposed to dust on wharfs as joiners, locksmiths, transport workers, isolators and in other trades. The beginning of the exposition was at least 15 years age, the duration of the exposition was 5 to 25 years. Two bronchial carcinomas and one mesothelioma of the pleura among our patients emphasize the increased risk for malignoma of the patients with asbestosis. For this reasons an early recognition and a regular medical control of the patients as well as a reducthe long time of latency between the beginning of the exposition and the appearance of morphologic changes it is recommended to employ older colleagues in the working places with dust exposition.", "contents": "[Pleural asbestosis in dock workers]. During the last three years 29 patients with asbestosis of the pleura were examined. 21 of them were exposed to dust on wharfs as joiners, locksmiths, transport workers, isolators and in other trades. The beginning of the exposition was at least 15 years age, the duration of the exposition was 5 to 25 years. Two bronchial carcinomas and one mesothelioma of the pleura among our patients emphasize the increased risk for malignoma of the patients with asbestosis. For this reasons an early recognition and a regular medical control of the patients as well as a reducthe long time of latency between the beginning of the exposition and the appearance of morphologic changes it is recommended to employ older colleagues in the working places with dust exposition."}
{"id": "PMID:483933", "title": "[Asthma caused by analgesics].", "content": "Asthma, caused by analgesics, a relatively frequent phenomenon in bronchial asthma conditioned by infection, often remains unrecognized and is then sometimes a danger of high degree for these patients. Pathogenetically, the asthma caused by analgesics perhaps does not underlie an immunological mechanism, but an induced by analgesics prostaglandin-E-synthesis inhibition. For this speak the results of skin tests, of the LTT with analgesics and of IgE concentration measurements. Concomitant phenomena of the asthma caused by analgesics are: initial rhinirrhoe, alcohol intolerance, polyposis nasi and NNH affections. Among the evoking noxae the pyrazolones (regard: pyrazolone containing asthma mixed preparations!) are of greatest importance. Since already the smallest doses of analgesics may evoke considerable asthmatic reactions the indication to the exposition test should be made cautiously. In an anamnestic suspicion on asthma caused by analgesics a strict avoidance of analgesics, antirheumatic drugs and antipyretic drugs is necessary. Salicyl amide does not cause in vitro a prostaglandin synthesis inhibition. Still further clinical examinations are necessary, whether it amy generally be recommended as a possible alternative of analgesics in asthma caused by analgesics.", "contents": "[Asthma caused by analgesics]. Asthma, caused by analgesics, a relatively frequent phenomenon in bronchial asthma conditioned by infection, often remains unrecognized and is then sometimes a danger of high degree for these patients. Pathogenetically, the asthma caused by analgesics perhaps does not underlie an immunological mechanism, but an induced by analgesics prostaglandin-E-synthesis inhibition. For this speak the results of skin tests, of the LTT with analgesics and of IgE concentration measurements. Concomitant phenomena of the asthma caused by analgesics are: initial rhinirrhoe, alcohol intolerance, polyposis nasi and NNH affections. Among the evoking noxae the pyrazolones (regard: pyrazolone containing asthma mixed preparations!) are of greatest importance. Since already the smallest doses of analgesics may evoke considerable asthmatic reactions the indication to the exposition test should be made cautiously. In an anamnestic suspicion on asthma caused by analgesics a strict avoidance of analgesics, antirheumatic drugs and antipyretic drugs is necessary. Salicyl amide does not cause in vitro a prostaglandin synthesis inhibition. Still further clinical examinations are necessary, whether it amy generally be recommended as a possible alternative of analgesics in asthma caused by analgesics."}
{"id": "PMID:483935", "title": "[Functional peripheral ischemia].", "content": "The terminal circulation which plays an important role in the thermoregulation particularly at the periphery of the extremities is not infrequently irritated by false multifactorial regulations. As their sequela an increased tonus of vasoconstrictors and pathological vasodilatation lead to idiopathic and symptomatic functional angiolopathies. Transitions into organic vascular processes are no rarity. From the extreme functional behaviour patterns complaints and symptoms of the individual angiolopathies may be derived. Clinic, differential diagnostics and therapeutic possibilities of the angiopathic reaction position, of the acrocyanosis and its variants, of the intermittent functional acrosyndromes and of the erythromelalgia are treated from practical points of view. In contrast to the secondary functional angiolopathies, in which the basic disease is of decisive importance in questions concerning expertise, the idiopathic forms alone are scarcely of high significance concerning the restriction of the physical function.", "contents": "[Functional peripheral ischemia]. The terminal circulation which plays an important role in the thermoregulation particularly at the periphery of the extremities is not infrequently irritated by false multifactorial regulations. As their sequela an increased tonus of vasoconstrictors and pathological vasodilatation lead to idiopathic and symptomatic functional angiolopathies. Transitions into organic vascular processes are no rarity. From the extreme functional behaviour patterns complaints and symptoms of the individual angiolopathies may be derived. Clinic, differential diagnostics and therapeutic possibilities of the angiopathic reaction position, of the acrocyanosis and its variants, of the intermittent functional acrosyndromes and of the erythromelalgia are treated from practical points of view. In contrast to the secondary functional angiolopathies, in which the basic disease is of decisive importance in questions concerning expertise, the idiopathic forms alone are scarcely of high significance concerning the restriction of the physical function."}
{"id": "PMID:483937", "title": "[The clinical significance of blood viscosity in degenerative angiopathies].", "content": "During an examination of a group of 63 patients with chronic ischaemic heart disease and condition after myocardial infarction the viscosities measured with the help of a rotation viscosimeter lay clearly above the values of good reference persons. There were positive correlations of the blood viscosity to the haematocrit value, fibrinogen level, globulin content as well as to the total protein, total lipid and cholesterol level. In the transition to the logarithm of the viscosity the measure for the linear dependence with the exception of the total protein level became smaller. A formation of partial correlation coefficients by means of the exclusion of the haematocrit in every case resulted in increasing coefficients r for the investigated parameters of the lipid metabolism cholesterol, total lipids and triglycerides.", "contents": "[The clinical significance of blood viscosity in degenerative angiopathies]. During an examination of a group of 63 patients with chronic ischaemic heart disease and condition after myocardial infarction the viscosities measured with the help of a rotation viscosimeter lay clearly above the values of good reference persons. There were positive correlations of the blood viscosity to the haematocrit value, fibrinogen level, globulin content as well as to the total protein, total lipid and cholesterol level. In the transition to the logarithm of the viscosity the measure for the linear dependence with the exception of the total protein level became smaller. A formation of partial correlation coefficients by means of the exclusion of the haematocrit in every case resulted in increasing coefficients r for the investigated parameters of the lipid metabolism cholesterol, total lipids and triglycerides."}
{"id": "PMID:483938", "title": "[Ultrasound diagnosis in arteriosclerosis obliterans].", "content": "From the standpoint of the clinical angiology the ultrasound Doppler method is of great diagnostic evidence. By means of this method depending on physico-technical and apparative prerequisites we succeed in judging the movements of blood in the non-invasive way. Of particular relevance is the sonographic investigation after Doppler of the extracranial part of arteries supplying the brain. In the region of extremities the poststenotic pressure measurings after Doppler give the possibility to establish early clinical stages and to objectify the degree of compensation of arterial circulatory obstructions.", "contents": "[Ultrasound diagnosis in arteriosclerosis obliterans]. From the standpoint of the clinical angiology the ultrasound Doppler method is of great diagnostic evidence. By means of this method depending on physico-technical and apparative prerequisites we succeed in judging the movements of blood in the non-invasive way. Of particular relevance is the sonographic investigation after Doppler of the extracranial part of arteries supplying the brain. In the region of extremities the poststenotic pressure measurings after Doppler give the possibility to establish early clinical stages and to objectify the degree of compensation of arterial circulatory obstructions."}
{"id": "PMID:483939", "title": "[Systolic time intervals during sequential ultrafiltration and hemodialysis].", "content": "The effects of a forced withdrawal of fluid by ultrafiltration and the influences of a connected haemodialysis on the period of isometric contraction of the heart were recorded by periodical registration during the treatment. A statistically certain decrease of the LVET (-19.2%) and an increase of the PEP (+ 11.1%) were found. Since at least in the period of the isolated ultrafiltration several essential parameters of the interior milieu do not change decisively, as cause for the changes observed of the systolic intervals of the heart period the decrease of the preload is discussed. During the period of ultrafiltration on an average 15.3 +/- 3.3 ml of plasma water were taken per minute.", "contents": "[Systolic time intervals during sequential ultrafiltration and hemodialysis]. The effects of a forced withdrawal of fluid by ultrafiltration and the influences of a connected haemodialysis on the period of isometric contraction of the heart were recorded by periodical registration during the treatment. A statistically certain decrease of the LVET (-19.2%) and an increase of the PEP (+ 11.1%) were found. Since at least in the period of the isolated ultrafiltration several essential parameters of the interior milieu do not change decisively, as cause for the changes observed of the systolic intervals of the heart period the decrease of the preload is discussed. During the period of ultrafiltration on an average 15.3 +/- 3.3 ml of plasma water were taken per minute."}
{"id": "PMID:483940", "title": "[Theoretical viewpoints of coronary hemodynamics relative to the localization of arterial stenoses and the collateral function with special reference to the so-called steal effect].", "content": "1. On a simple branched model of the vascular system is deduced that the restriction of the reserve of blood supply (coronary reserve) occurring in arterial constrictions depends not only on the degree of constrictions, but also on their type of distribution, by means of which peculiarities of the univascular diseases in contrast to the multivascular diseases are the result. 2. Constrictions further the formation of the collateral circulation, the size of which in the same initial pressure increases with the degree of constriction and the decrease of the peripheral resistance. 3. The effects of the collateral circulation are fully different for proximally and distally localized stenoses. Proximal stenoses lead the collateral circulation in direction to the narrowed area, peripheral narrowings of the circulation, however, to the counter-direction. 4. A Steal effect is possible only in peripheral narrowings of the circulation. Its haemodynamic conditions are described in detail. 5. As haemodynamic explanation for the positive dipyridamol test the hypothesis arises that it might refer to remarkable peripheral constrictions. 6. The application of vasodilators is from the haemodynamic point of view indicated above all in proximal constrictions, when distally from this there are interarterial anastomoses. Repression is necessary in peripheral narrowings, when their reserve of blood supply is already consumed by vasodilatation. In these cases is to be reckoned with a Steal effect.", "contents": "[Theoretical viewpoints of coronary hemodynamics relative to the localization of arterial stenoses and the collateral function with special reference to the so-called steal effect]. 1. On a simple branched model of the vascular system is deduced that the restriction of the reserve of blood supply (coronary reserve) occurring in arterial constrictions depends not only on the degree of constrictions, but also on their type of distribution, by means of which peculiarities of the univascular diseases in contrast to the multivascular diseases are the result. 2. Constrictions further the formation of the collateral circulation, the size of which in the same initial pressure increases with the degree of constriction and the decrease of the peripheral resistance. 3. The effects of the collateral circulation are fully different for proximally and distally localized stenoses. Proximal stenoses lead the collateral circulation in direction to the narrowed area, peripheral narrowings of the circulation, however, to the counter-direction. 4. A Steal effect is possible only in peripheral narrowings of the circulation. Its haemodynamic conditions are described in detail. 5. As haemodynamic explanation for the positive dipyridamol test the hypothesis arises that it might refer to remarkable peripheral constrictions. 6. The application of vasodilators is from the haemodynamic point of view indicated above all in proximal constrictions, when distally from this there are interarterial anastomoses. Repression is necessary in peripheral narrowings, when their reserve of blood supply is already consumed by vasodilatation. In these cases is to be reckoned with a Steal effect."}
{"id": "PMID:483941", "title": "[Analysis of the causes of death in patients with rheumatoid arthritis].", "content": "The post-mortem examinations performed from 1943 to 1977 in Tartu were examined for the frequency of rheumatoid arthritis as cause of death. Here was the result that since 1968 in increasing number this clinical picture was observed and that during the last five years it appeared in 1.4% of the cases. As cause of death in patients whose basic disease was rheumatoid arthritis most frequently and uraemia was found either on the basis of an amyloidosis of the kidneys or of a chronic glomerulonephritis. Also the number of fatal complications among a long-lasting glucocorticosteroid therapy is not unconsiderable.", "contents": "[Analysis of the causes of death in patients with rheumatoid arthritis]. The post-mortem examinations performed from 1943 to 1977 in Tartu were examined for the frequency of rheumatoid arthritis as cause of death. Here was the result that since 1968 in increasing number this clinical picture was observed and that during the last five years it appeared in 1.4% of the cases. As cause of death in patients whose basic disease was rheumatoid arthritis most frequently and uraemia was found either on the basis of an amyloidosis of the kidneys or of a chronic glomerulonephritis. Also the number of fatal complications among a long-lasting glucocorticosteroid therapy is not unconsiderable."}
{"id": "PMID:483942", "title": "[Radiological methods in arteriosclerosis obliterans].", "content": "Of the X-ray methods for the demonstration of disturbances of the blood supply only the angiography is of practical importance. The proof of vascular calcium on so-called X-ray pictures of the soft parts informs about wall processes, but says absolutely nothing about a disturbed flow. The diagnosis of an obliterating vascular disease is still successful on the extremities with the help of the simple clinical means of pulse palpation, auscultation, measuring of the blood pressure with comparison of the sides, particularly when load methods are included. But the angiography is the only diagnostic method which makes recognizable localisation, extension and degree of obliterating changes intra vitam. Nevertheless it stands at the end of a diagnostic chain and, as far as the ischaemia of the extremities is concerned, it is absolutely indicated only as preoperative method. The development of newer therapeutic methods by means of the saving catheter technique for the restoration of the vascular system is described in short and outlined in its tendencies.", "contents": "[Radiological methods in arteriosclerosis obliterans]. Of the X-ray methods for the demonstration of disturbances of the blood supply only the angiography is of practical importance. The proof of vascular calcium on so-called X-ray pictures of the soft parts informs about wall processes, but says absolutely nothing about a disturbed flow. The diagnosis of an obliterating vascular disease is still successful on the extremities with the help of the simple clinical means of pulse palpation, auscultation, measuring of the blood pressure with comparison of the sides, particularly when load methods are included. But the angiography is the only diagnostic method which makes recognizable localisation, extension and degree of obliterating changes intra vitam. Nevertheless it stands at the end of a diagnostic chain and, as far as the ischaemia of the extremities is concerned, it is absolutely indicated only as preoperative method. The development of newer therapeutic methods by means of the saving catheter technique for the restoration of the vascular system is described in short and outlined in its tendencies."}
{"id": "PMID:483943", "title": "[Conservative treatment of arteriosclerosis obliterans].", "content": "The conservative therapy of the arteriosclerotic obstructive disease demands a complex approach. The basis therapy, the symptomatic treatment of the haemodynamics of the extremity with ill vessels, the pathogenetically oriented therapy as well as the treatment of other localisations of the arteriosclerosis should be used combined. At stage III and IV additional measures, such as combat against pain, therapy with antibiotics and local treatment of tissue lesions are necessary. The results of the therapy should be critically tested not only on the basis of the clinical picture, but also on the basis of the course of the vascular processes.", "contents": "[Conservative treatment of arteriosclerosis obliterans]. The conservative therapy of the arteriosclerotic obstructive disease demands a complex approach. The basis therapy, the symptomatic treatment of the haemodynamics of the extremity with ill vessels, the pathogenetically oriented therapy as well as the treatment of other localisations of the arteriosclerosis should be used combined. At stage III and IV additional measures, such as combat against pain, therapy with antibiotics and local treatment of tissue lesions are necessary. The results of the therapy should be critically tested not only on the basis of the clinical picture, but also on the basis of the course of the vascular processes."}
{"id": "PMID:483944", "title": "[The effect of Ca++ on gastrin output and LESP before and after antrectomy (author's transl)].", "content": "Previous investigations show that calcium infusion stimulates gastrin output in healthy volunteers, whereas after antrectomy calcium does not cause any significant change of IRG levels. Both healthy volunteers and antrectomized patients (B II) show a continuous increase of LESP, reaching a maximum after 120 minutes. These results suggest a direct interaction of Ca++-ions and gastrointestinal smooth muscle cells.", "contents": "[The effect of Ca++ on gastrin output and LESP before and after antrectomy (author's transl)]. Previous investigations show that calcium infusion stimulates gastrin output in healthy volunteers, whereas after antrectomy calcium does not cause any significant change of IRG levels. Both healthy volunteers and antrectomized patients (B II) show a continuous increase of LESP, reaching a maximum after 120 minutes. These results suggest a direct interaction of Ca++-ions and gastrointestinal smooth muscle cells."}
{"id": "PMID:483945", "title": "[Portal hypertension in idiopathic haemochromatosis (author's transl)].", "content": "In contrast to hepatic and alcoholic cirrhosis of the liver, the life-threatening portal hypertension seen in idiopathic haemochromatosis (IH) responds well to therapy. With the aid of laparoscopic transhepatic manometry, we determined the pressures in the branches of the portal vein and the hepatic vein in four patients presenting with IH, and compared the values obtained with clinico-chemical and histological parameters. Three patients who had been suffering from the disease for periods varying between two and ten years, responded well to therapy. The fourth patient, who presented with the highest pressure in the portal vein (26.5 mm Hg) died, three months after the initial establishment of the diagnosis of advanced IH, in consequence of unmanagable haemorrhage from oesophageal varices.", "contents": "[Portal hypertension in idiopathic haemochromatosis (author's transl)]. In contrast to hepatic and alcoholic cirrhosis of the liver, the life-threatening portal hypertension seen in idiopathic haemochromatosis (IH) responds well to therapy. With the aid of laparoscopic transhepatic manometry, we determined the pressures in the branches of the portal vein and the hepatic vein in four patients presenting with IH, and compared the values obtained with clinico-chemical and histological parameters. Three patients who had been suffering from the disease for periods varying between two and ten years, responded well to therapy. The fourth patient, who presented with the highest pressure in the portal vein (26.5 mm Hg) died, three months after the initial establishment of the diagnosis of advanced IH, in consequence of unmanagable haemorrhage from oesophageal varices."}
{"id": "PMID:483946", "title": "[Cervical ripening in late pregnancy and at term].", "content": "Cervical ripening before labors is not an exclusively passive process by uterine contractions. On the contrary, we can find a lot of highly active processes in the cervical connective tissue with changes in metabolism of the cells leading to changes in the ground substance and the organisation and structure of collagenous fibres. We do not as yet know the trigger mechanisms of the prepartum cervical rpiening. Probably prostaglandins and relaxin are such triggering substances. At present, our new understanding of this active cervical process does not help the obstetrician. There is no laboratory test up to now. Cervical ripeness can only be examined on the basis of clinical criteria: length, consistency, position, and dilatation of the cervix uteri and station of the fetal head. The most usual score of cervical ripeness is Bishop's Pelvic-score. A high scoring excludes failures of induction of labor. But the rate of wrong prognosis, concerning efficiency of induction and time of labor is relatively high in cases with low rating of cervical ripeness. A more differentiated scoring of some of the criteria and some weighing of these seems to lead to better prognosis. On the other hand, recent clinical experiences with prostaglandins lead us to expect that we will be able to induce cervical ripeness.", "contents": "[Cervical ripening in late pregnancy and at term]. Cervical ripening before labors is not an exclusively passive process by uterine contractions. On the contrary, we can find a lot of highly active processes in the cervical connective tissue with changes in metabolism of the cells leading to changes in the ground substance and the organisation and structure of collagenous fibres. We do not as yet know the trigger mechanisms of the prepartum cervical rpiening. Probably prostaglandins and relaxin are such triggering substances. At present, our new understanding of this active cervical process does not help the obstetrician. There is no laboratory test up to now. Cervical ripeness can only be examined on the basis of clinical criteria: length, consistency, position, and dilatation of the cervix uteri and station of the fetal head. The most usual score of cervical ripeness is Bishop's Pelvic-score. A high scoring excludes failures of induction of labor. But the rate of wrong prognosis, concerning efficiency of induction and time of labor is relatively high in cases with low rating of cervical ripeness. A more differentiated scoring of some of the criteria and some weighing of these seems to lead to better prognosis. On the other hand, recent clinical experiences with prostaglandins lead us to expect that we will be able to induce cervical ripeness."}
{"id": "PMID:483947", "title": "[The influence of highly purified hCG on the immunocompetence of human lymphocytes and DNA repair system].", "content": "PHA-stimulation of human T-lymphocytes, a parameter of cellular immunocompetence, can be suppressed by highly purified human chorionic gonadotropin (hCG) in a dose-dependent manner. This was shown spectrophotometrically and by the mitosis index. Our results are in agreement with H3-thymidine incorporation studies. We further investigated the DNA repair system in UV-light- and hCG-explsed lymphocytes. It could be shown that DNA repair-replication is disturbed by hCG. Such repair disturbances may cause reduced H3-thymidine incorporation in PHA-stimulated and hCG-treated lymphocytes. It is probably on this molecular level that hCG influences the immunocompetence of human lymphocytes.", "contents": "[The influence of highly purified hCG on the immunocompetence of human lymphocytes and DNA repair system]. PHA-stimulation of human T-lymphocytes, a parameter of cellular immunocompetence, can be suppressed by highly purified human chorionic gonadotropin (hCG) in a dose-dependent manner. This was shown spectrophotometrically and by the mitosis index. Our results are in agreement with H3-thymidine incorporation studies. We further investigated the DNA repair system in UV-light- and hCG-explsed lymphocytes. It could be shown that DNA repair-replication is disturbed by hCG. Such repair disturbances may cause reduced H3-thymidine incorporation in PHA-stimulated and hCG-treated lymphocytes. It is probably on this molecular level that hCG influences the immunocompetence of human lymphocytes."}
{"id": "PMID:483948", "title": "[The pre-ejection-period of the humen fetal heart: significance of base-line alterations in perinatal period].", "content": "The Pre-ejection period of the cardiac cycle, fetal heart rate and uterine contractions were monitored in a series of 115 unselected fetus sub partu. A new one-line technique permits a continuous registration of PEP patterns simultaneously with the CTG. Any change in base-line of the PEP war related to heart rate, acid-base-status and gestational age. The average value of PEP in fetus was 72,93 +/- 7,9 msec. The base-line of PEP increased paralelly with gestational age of fetus and decreased rapidly beyond normal values after the 41. week of gestation. Within normal values alterations in base-line of the PEP were negativly correlated to alterations in base-line of the heart rate. Abnormal high and low base-lines of the PEP were observed in fetal acidosis, hypoxemia, and in uteroplacental insufficiency. The prolonging or shortening of the PEP base-line seems to signalize an acute injury of the utero-feto-placental unit in perinatal period.", "contents": "[The pre-ejection-period of the humen fetal heart: significance of base-line alterations in perinatal period]. The Pre-ejection period of the cardiac cycle, fetal heart rate and uterine contractions were monitored in a series of 115 unselected fetus sub partu. A new one-line technique permits a continuous registration of PEP patterns simultaneously with the CTG. Any change in base-line of the PEP war related to heart rate, acid-base-status and gestational age. The average value of PEP in fetus was 72,93 +/- 7,9 msec. The base-line of PEP increased paralelly with gestational age of fetus and decreased rapidly beyond normal values after the 41. week of gestation. Within normal values alterations in base-line of the PEP were negativly correlated to alterations in base-line of the heart rate. Abnormal high and low base-lines of the PEP were observed in fetal acidosis, hypoxemia, and in uteroplacental insufficiency. The prolonging or shortening of the PEP base-line seems to signalize an acute injury of the utero-feto-placental unit in perinatal period."}
{"id": "PMID:483949", "title": "Selection criteria for antenatal cardiotocography.", "content": "The present report considers two questions: 1) Which patients should be monitored routinely and 2) the interpretation of antenatal cardiotocography. During 26 months 812 patients with risk pregnancies were monitored with antenatal CTG. Most of them were daily registered. One hundred and seventeen (14.5%) showed pathological CTG changes. The CTG patterns were put into four classes according to the degree of seriousness. A comparison between high risk and low risk pregnancies showed that nearly all abnormal CTGs occurred in the high risk group. Within the high risk group, patients with essential hypertension and intrauterine growth retardation had pathological CTG changes in 20--30%. The study shows that high risk pregnancies should be monitored regularly at short intervals to allow early diagnosis of fetal distress. The value of routine CTG in low risk pregnancy can be questioned.", "contents": "Selection criteria for antenatal cardiotocography. The present report considers two questions: 1) Which patients should be monitored routinely and 2) the interpretation of antenatal cardiotocography. During 26 months 812 patients with risk pregnancies were monitored with antenatal CTG. Most of them were daily registered. One hundred and seventeen (14.5%) showed pathological CTG changes. The CTG patterns were put into four classes according to the degree of seriousness. A comparison between high risk and low risk pregnancies showed that nearly all abnormal CTGs occurred in the high risk group. Within the high risk group, patients with essential hypertension and intrauterine growth retardation had pathological CTG changes in 20--30%. The study shows that high risk pregnancies should be monitored regularly at short intervals to allow early diagnosis of fetal distress. The value of routine CTG in low risk pregnancy can be questioned."}
{"id": "PMID:483950", "title": "Vaginal prostaglandin E2 for interruption of pregnancy and management of intrauterine death.", "content": "Vaginal suppositories containing 20 mg prostaglanding E2, administered at 2-5h intervals, are very effective in interrupting second-trimester pregnancy and in inducing labor in case of death in utero. However, side effects are common and make the treatment unpleasant to most patients. Premedication with an maintenance of a potent anti-emetic (e.g. haloperidol) and an anti-diarrheic (e.g. loperamide) considerably reduce the frequency and severity of these side effects.", "contents": "Vaginal prostaglandin E2 for interruption of pregnancy and management of intrauterine death. Vaginal suppositories containing 20 mg prostaglanding E2, administered at 2-5h intervals, are very effective in interrupting second-trimester pregnancy and in inducing labor in case of death in utero. However, side effects are common and make the treatment unpleasant to most patients. Premedication with an maintenance of a potent anti-emetic (e.g. haloperidol) and an anti-diarrheic (e.g. loperamide) considerably reduce the frequency and severity of these side effects."}
{"id": "PMID:483951", "title": "[Plasma levels of PGE2, PGA2 and PGF2 alpha during spontaneous labour].", "content": "Plasma levels of PGE2, PGA2 and PGF2 alpha were determined by radioimmunoassay in maternal venous blood during different phases of uterine contractions. Mean prostaglandin levels during labor were: PGE2 = 2,25 +/- 1,3 ng/ml (mean +/- SD), PGA2 = 2,86 +/- 1,55 ng/ml and PGF2 alpha = 0,58 +/- 0,37 ng/ml. The approximate ratios between the prostaglandin concentrations were: PGE2:PGA2: PGF2 alpha = 1:1-3:0,3. No changes of PGE2 levels were found in the course of concentrations during the first and second stage of labor. At delivery and at the contraction followed by the expulsion of the placenta, PGE2 concentrations rose from the beginning of the contraction (2,21 +/- 0,07 ng PGE2/ml) to the maximum of the contraction (4,31 +/- 0,56 ng PGE2/ml). PGA2 levels did neither show a correlation to the course of contractions nor to the different stages of labor, but were considerably different between patients during the first stage of labor (e.g. 1,9 +/- 1,1 ng PGA2/ml) and during the second stage of labor (3,36 +/- 2,8 ng PGA2/ml). Mean PGF2 alpha concentrations were between 0,21 +/- 0,12 ngPGF2 alpha/ml (1st stage) and 0,81 +/- 0,11 ng PGF2alpha/ml (highest value obtained, 1st stage of labor, too). There was no correlation between PGF2 alpha concentrations and the course of contractions during the different stages of labor.", "contents": "[Plasma levels of PGE2, PGA2 and PGF2 alpha during spontaneous labour]. Plasma levels of PGE2, PGA2 and PGF2 alpha were determined by radioimmunoassay in maternal venous blood during different phases of uterine contractions. Mean prostaglandin levels during labor were: PGE2 = 2,25 +/- 1,3 ng/ml (mean +/- SD), PGA2 = 2,86 +/- 1,55 ng/ml and PGF2 alpha = 0,58 +/- 0,37 ng/ml. The approximate ratios between the prostaglandin concentrations were: PGE2:PGA2: PGF2 alpha = 1:1-3:0,3. No changes of PGE2 levels were found in the course of concentrations during the first and second stage of labor. At delivery and at the contraction followed by the expulsion of the placenta, PGE2 concentrations rose from the beginning of the contraction (2,21 +/- 0,07 ng PGE2/ml) to the maximum of the contraction (4,31 +/- 0,56 ng PGE2/ml). PGA2 levels did neither show a correlation to the course of contractions nor to the different stages of labor, but were considerably different between patients during the first stage of labor (e.g. 1,9 +/- 1,1 ng PGA2/ml) and during the second stage of labor (3,36 +/- 2,8 ng PGA2/ml). Mean PGF2 alpha concentrations were between 0,21 +/- 0,12 ngPGF2 alpha/ml (1st stage) and 0,81 +/- 0,11 ng PGF2alpha/ml (highest value obtained, 1st stage of labor, too). There was no correlation between PGF2 alpha concentrations and the course of contractions during the different stages of labor."}
{"id": "PMID:483952", "title": "[Serumprolactin and lactation in a case of placental sulfatase deficiency].", "content": "In a pregnancy with placental sulfatase deficiency very low serum concentrations of estrone and estradiol-1 7 beta are found. Estriol is not detectable. In spite of this serum concentrations of prolactin were in the normal pregnancy range. There was no premature lactation in pregnancy. Lactation in puerperium was unsatisfactory.", "contents": "[Serumprolactin and lactation in a case of placental sulfatase deficiency]. In a pregnancy with placental sulfatase deficiency very low serum concentrations of estrone and estradiol-1 7 beta are found. Estriol is not detectable. In spite of this serum concentrations of prolactin were in the normal pregnancy range. There was no premature lactation in pregnancy. Lactation in puerperium was unsatisfactory."}
{"id": "PMID:483953", "title": "[Unilateral hyperplasia of mammae during pregnancy].", "content": "The article reports on a case of marked unilateral hyperplasia of the mammae during pregnancy. Literature references show that this is a rare occurrence. The possible complications require an intensive control. Although malignant degeneration has not been reported, the authors believe that diagnostic clarification is mandatory.", "contents": "[Unilateral hyperplasia of mammae during pregnancy]. The article reports on a case of marked unilateral hyperplasia of the mammae during pregnancy. Literature references show that this is a rare occurrence. The possible complications require an intensive control. Although malignant degeneration has not been reported, the authors believe that diagnostic clarification is mandatory."}
{"id": "PMID:483955", "title": "[Naevus spongiosus albus mucosae. Review and personal observations].", "content": "White sponge nevus of the mucosa is a rare, mostly verifiable hereditary ectodermal dysplasia of the mucous membranes. Because associated symptoms of the skin are missing, it is classified as a distinctive disease among the congenital leukokeratoses. In the most cases the symptomless mucosal lesions are detected by dentological examination though they are present at birth or appear in the first years of life. On the basis of three own observations clinical appearance, histopathology, electronmicroscopy and differential diagnosis are discussed.", "contents": "[Naevus spongiosus albus mucosae. Review and personal observations]. White sponge nevus of the mucosa is a rare, mostly verifiable hereditary ectodermal dysplasia of the mucous membranes. Because associated symptoms of the skin are missing, it is classified as a distinctive disease among the congenital leukokeratoses. In the most cases the symptomless mucosal lesions are detected by dentological examination though they are present at birth or appear in the first years of life. On the basis of three own observations clinical appearance, histopathology, electronmicroscopy and differential diagnosis are discussed."}
{"id": "PMID:483956", "title": "[LEOPARD syndrome].", "content": "Three cases of LEOPARD-syndrome are presented. A review of literature is given. Among the symptoms of the syndrome lentigines, ECG-abnormalities, pulmonal-stenosis, retardation of growth and deafness show the strongest penetrance and are most constantly found.", "contents": "[LEOPARD syndrome]. Three cases of LEOPARD-syndrome are presented. A review of literature is given. Among the symptoms of the syndrome lentigines, ECG-abnormalities, pulmonal-stenosis, retardation of growth and deafness show the strongest penetrance and are most constantly found."}
{"id": "PMID:483961", "title": "[Prediction of the effects of client-centered psychotherapy on the basis of an indications interview].", "content": "Several effects of client-centered therapy were predicted on the basis of a short intake-interview divided into two parts: an exploration-phase and a quasi-therapy-phase. Elements of the international behavior and elements of the intrapersonal exploration of the client were rated by neutral observers and served as predictor variables as well as ratings of the experiences in the two interview situations by the client himself. Our population were 29 out-patients of a psychiatric clinic. We could demonstrate, that on the basis of an 45-minutes intake-interview it is possible to make a rather good prediction of the therapy outcome -- measured by several clinically relevant personality scales -- though therapists and interviewer were not identical and though there was a waiting period of several weeks between the interview and the start of therapy and though the therapists were rather unexperienced.", "contents": "[Prediction of the effects of client-centered psychotherapy on the basis of an indications interview]. Several effects of client-centered therapy were predicted on the basis of a short intake-interview divided into two parts: an exploration-phase and a quasi-therapy-phase. Elements of the international behavior and elements of the intrapersonal exploration of the client were rated by neutral observers and served as predictor variables as well as ratings of the experiences in the two interview situations by the client himself. Our population were 29 out-patients of a psychiatric clinic. We could demonstrate, that on the basis of an 45-minutes intake-interview it is possible to make a rather good prediction of the therapy outcome -- measured by several clinically relevant personality scales -- though therapists and interviewer were not identical and though there was a waiting period of several weeks between the interview and the start of therapy and though the therapists were rather unexperienced."}
{"id": "PMID:483962", "title": "[Ideology and education (influence): notices on the pseudo-question on medical and non-medical psychoanalysis].", "content": "Argumentations pro or contra \"medical\" or \"non-medical\" psychoanalysis are rarely free from ideologies. Only in the stage of psychoanalytical self-cognition the question becomes another one. It must be differentiated between the educational background and training of the psychoanalyst. It is just the educational background (academica finally examination in medicine, psychologie, theologie etc.) which produces attitudes, which can form (influence) the training in a wrong way. Such discussions become easily a pseudo-question because of a narrow class-arrogance. The urgency of using psychoanalytical concepts and methods in the social field should help to reconcile contrasts.", "contents": "[Ideology and education (influence): notices on the pseudo-question on medical and non-medical psychoanalysis]. Argumentations pro or contra \"medical\" or \"non-medical\" psychoanalysis are rarely free from ideologies. Only in the stage of psychoanalytical self-cognition the question becomes another one. It must be differentiated between the educational background and training of the psychoanalyst. It is just the educational background (academica finally examination in medicine, psychologie, theologie etc.) which produces attitudes, which can form (influence) the training in a wrong way. Such discussions become easily a pseudo-question because of a narrow class-arrogance. The urgency of using psychoanalytical concepts and methods in the social field should help to reconcile contrasts."}
{"id": "PMID:483963", "title": "[Why has psychotherapy failed in its social mission?].", "content": "The following are the principal reasons why psychotherapy has not lived up to expectations in respect to its social task: its misunderstanding of itself in that it strives to be a science or a teaching of cure, whereas it can and should be the teaching of adequate address and reply; its fatal mistaking of materialism for substantiality; its submission to the pressure of public opinion and its slogan: I am good, because I am well. I am well, because I am good; Its ambition to know the person (\"I know who you are\"), instead of aiming to understand him (\"I know what you mean\"); its conception rigidly set on alternative -- and antipodal thinking; its equation of good equals healthy; its rigidly one-sided stance in the matter of fees, in the matter of financial problems in general and towards the insured and insurance; its isolationism and its exclusivity.", "contents": "[Why has psychotherapy failed in its social mission?]. The following are the principal reasons why psychotherapy has not lived up to expectations in respect to its social task: its misunderstanding of itself in that it strives to be a science or a teaching of cure, whereas it can and should be the teaching of adequate address and reply; its fatal mistaking of materialism for substantiality; its submission to the pressure of public opinion and its slogan: I am good, because I am well. I am well, because I am good; Its ambition to know the person (\"I know who you are\"), instead of aiming to understand him (\"I know what you mean\"); its conception rigidly set on alternative -- and antipodal thinking; its equation of good equals healthy; its rigidly one-sided stance in the matter of fees, in the matter of financial problems in general and towards the insured and insurance; its isolationism and its exclusivity."}
{"id": "PMID:483964", "title": "[The relationship between mental health, socialization and creativity].", "content": "It is shown, that there are two traditions of thought in research as well as in psychotherapy which foster different assumptions about the relationship between a persons creativity and his mental well-being and between his creativity and the child-rearing practice of his parents. One tradition suggests that most creative people have experienced more intense suffering during childhood than others. The other tradition takes the opposite position, that a permissive, non-authoritarian home environment is most conducive for later creativity. Results of empirical research relevant to this questions have been contradictory, partly due to considerable methodological difficulties, and partly due to the ideological biasis of the experimenters and reviewers. Our own research on the child rearing practices and attitudes related to creative/non-creative 12-year old children of both sexes, as measured by questionnaires and observations of mother-child interaction, opposes the latter tradition. We have observed that mothers of high creative children behave more active and evaluating when engaged in dyadic problem solving situations with their children, and this pattern is symmetrically reflected in the behavior of their children. Mothers of low creative children, however, interact in a more guarded and cautious manner, yet with more visible demonstrations of positive affect. Questionnaire data on the fathers' attitudes on child-rearing seems to oppose the belief that creative children have permissive, non-authoritarian fathers.", "contents": "[The relationship between mental health, socialization and creativity]. It is shown, that there are two traditions of thought in research as well as in psychotherapy which foster different assumptions about the relationship between a persons creativity and his mental well-being and between his creativity and the child-rearing practice of his parents. One tradition suggests that most creative people have experienced more intense suffering during childhood than others. The other tradition takes the opposite position, that a permissive, non-authoritarian home environment is most conducive for later creativity. Results of empirical research relevant to this questions have been contradictory, partly due to considerable methodological difficulties, and partly due to the ideological biasis of the experimenters and reviewers. Our own research on the child rearing practices and attitudes related to creative/non-creative 12-year old children of both sexes, as measured by questionnaires and observations of mother-child interaction, opposes the latter tradition. We have observed that mothers of high creative children behave more active and evaluating when engaged in dyadic problem solving situations with their children, and this pattern is symmetrically reflected in the behavior of their children. Mothers of low creative children, however, interact in a more guarded and cautious manner, yet with more visible demonstrations of positive affect. Questionnaire data on the fathers' attitudes on child-rearing seems to oppose the belief that creative children have permissive, non-authoritarian fathers."}
{"id": "PMID:483965", "title": "[Reality in the sciences of humans].", "content": "The claim of science, which concerns directly human living, to be \"objective\" and \"universal\" culminates in the belief, scientific methods could represent reality. Such a science puts men in a couple of scientific laws and suppresses the moment of active doing (accepting or refusing) as a sufficient preassumption of reality. In an alternative way we are looking for a science where reality means invitation to perform own doing.", "contents": "[Reality in the sciences of humans]. The claim of science, which concerns directly human living, to be \"objective\" and \"universal\" culminates in the belief, scientific methods could represent reality. Such a science puts men in a couple of scientific laws and suppresses the moment of active doing (accepting or refusing) as a sufficient preassumption of reality. In an alternative way we are looking for a science where reality means invitation to perform own doing."}
{"id": "PMID:483966", "title": "[Statements of a patient concerning his writer's cramp].", "content": "The spontaneous statements a patient with writers' cramp made in analytic psychotherapy about the relatively slight motor disorder of his right hand were recorded. This immediate presentation of the relations between the patient's person and his disorder were compared with investigations and reports about patients with writer's cramp in psychotherapy. Similarities and differences in the views of the patient and of the therapists about the relation between person and symptom were shown. This was done under special evaluation of the categories of interpersonal communication, developed by D. Wyss in his \"integrative psychotherapy\".", "contents": "[Statements of a patient concerning his writer's cramp]. The spontaneous statements a patient with writers' cramp made in analytic psychotherapy about the relatively slight motor disorder of his right hand were recorded. This immediate presentation of the relations between the patient's person and his disorder were compared with investigations and reports about patients with writer's cramp in psychotherapy. Similarities and differences in the views of the patient and of the therapists about the relation between person and symptom were shown. This was done under special evaluation of the categories of interpersonal communication, developed by D. Wyss in his \"integrative psychotherapy\"."}
{"id": "PMID:483971", "title": "Clinical and histologic observations of actively induced resistance to Eimeria ferrisi Levine and Ivens, 1965 (Protozoa: Eimeriidae) in the mouse (Mus musculus).", "content": "Actively induced resistance to Eimeria ferrisi was studied clinically and histologically in Mus musculus. Results indicated that a partial resistance to challenge was obtained. Initially infected animals had severe symptoms of coccidiosis but the symptoms diminished as the infections progressed. Previously infected mice had only slight symptoms of infection when challenged. Challenge doses produced severe coccidiosis in the non-immune controls although none died. Six mice died at peak patency during the immunizing inoculations. Histologic examination indicated that parasite numbers were reduced in resistant animals. The reduction appeared greatest during late sexual development. Tissues from resistant animals showed little evidence of damage and appeared to contain an increased amount of lymphoid tissue. Tissues from non-immunized mice killed after challenge were heavily infected and contained extruded blood, mucous, and cellular debris in the gut lumen. Considerable destruction of host epithelial cells occurred. The observations are discussed and compared to other coccidial immunity studies.", "contents": "Clinical and histologic observations of actively induced resistance to Eimeria ferrisi Levine and Ivens, 1965 (Protozoa: Eimeriidae) in the mouse (Mus musculus). Actively induced resistance to Eimeria ferrisi was studied clinically and histologically in Mus musculus. Results indicated that a partial resistance to challenge was obtained. Initially infected animals had severe symptoms of coccidiosis but the symptoms diminished as the infections progressed. Previously infected mice had only slight symptoms of infection when challenged. Challenge doses produced severe coccidiosis in the non-immune controls although none died. Six mice died at peak patency during the immunizing inoculations. Histologic examination indicated that parasite numbers were reduced in resistant animals. The reduction appeared greatest during late sexual development. Tissues from resistant animals showed little evidence of damage and appeared to contain an increased amount of lymphoid tissue. Tissues from non-immunized mice killed after challenge were heavily infected and contained extruded blood, mucous, and cellular debris in the gut lumen. Considerable destruction of host epithelial cells occurred. The observations are discussed and compared to other coccidial immunity studies."}
{"id": "PMID:483974", "title": "The relation between skin histamine concentration, histamine sensitivity, and the resistance of cattle to the tick, Boophilus microplus.", "content": "Cattle with different degrees of resistance to Boophilus microplus have responses to tick allergen which correlate with their resistance level. The total amount of histamine in the skin also correlates with both resistance and the immediate hypersensitivity reactions, but the sensitivity to injected histamine does not. Treatment with the antihistamine drug mepyramine maleate suppresses the cutaneous hypersensitivity reactions. The results suggest that the main pharmacologically active agent in these reactions is histamine, and that the total amount of it available locally in the skin may have a role in the resistance to this parasite.", "contents": "The relation between skin histamine concentration, histamine sensitivity, and the resistance of cattle to the tick, Boophilus microplus. Cattle with different degrees of resistance to Boophilus microplus have responses to tick allergen which correlate with their resistance level. The total amount of histamine in the skin also correlates with both resistance and the immediate hypersensitivity reactions, but the sensitivity to injected histamine does not. Treatment with the antihistamine drug mepyramine maleate suppresses the cutaneous hypersensitivity reactions. The results suggest that the main pharmacologically active agent in these reactions is histamine, and that the total amount of it available locally in the skin may have a role in the resistance to this parasite."}
{"id": "PMID:483975", "title": "[On Cheyletiella infestation in domestic cats (author's transl)].", "content": "Two Persian cats kept in common, 3 months and 2 years old, presented a heavy infestation with Cheyletiella mites. The cats showed itching and loss of hair. The animals' owners suffered from severe itching and redish papulae, particularly on those parts of the body which frequently got in contact with the cats. After three treatments with Alugan powder (Hoechst) the cats were freed from the mite infestation. The owners' skin affection disappeared soon without any treatment. This is the first recovery of Cheyletiella mites on cats in Austria. The mites were identified as C. blakei, although obvious differences between the first description and our material were encountered. C. blakei could be primarily separated from C. parasitivorax according to the female tectum and the peritremata. Typically the sense organs on the genua of the first pair of legs are ovoid in shape. This feature, although morphologically variable, could be used as a diagnostic aid, if enough adult specimens are available.", "contents": "[On Cheyletiella infestation in domestic cats (author's transl)]. Two Persian cats kept in common, 3 months and 2 years old, presented a heavy infestation with Cheyletiella mites. The cats showed itching and loss of hair. The animals' owners suffered from severe itching and redish papulae, particularly on those parts of the body which frequently got in contact with the cats. After three treatments with Alugan powder (Hoechst) the cats were freed from the mite infestation. The owners' skin affection disappeared soon without any treatment. This is the first recovery of Cheyletiella mites on cats in Austria. The mites were identified as C. blakei, although obvious differences between the first description and our material were encountered. C. blakei could be primarily separated from C. parasitivorax according to the female tectum and the peritremata. Typically the sense organs on the genua of the first pair of legs are ovoid in shape. This feature, although morphologically variable, could be used as a diagnostic aid, if enough adult specimens are available."}
{"id": "PMID:484024", "title": "[Dynamics of the statistical indices of evoked potentials in modally specific brain structures during the formation of defensive reflexes].", "content": "Interdependence of integrative processes in the relays of the analyzers of signal stimuli (auditory and visual) was estimated by EP parameters (latency, amplitude, and duration) of the first positive and negative components. It has been shown that the significance of the coefficients of correlation between EP parameters in the studied structures is determined by the stage of the behaviour formation; it also depends on the EP component chosen for the analysis. Significant correlation coefficients are timed to the active period in the formation of defensive conditioned reaction. In this period significant correlation coefficients for the parameters of the first positive EP component are established in various fields of cortical projection zones and between the parameters of the negative component in all relays of the analyzer. The data are treated from the viewpoint of adaptation of the analyser activity to the organism's needs at each stage in the formation adaptive behaviour and of the role of motivational influences in the organization of intraanalyzer integrations.", "contents": "[Dynamics of the statistical indices of evoked potentials in modally specific brain structures during the formation of defensive reflexes]. Interdependence of integrative processes in the relays of the analyzers of signal stimuli (auditory and visual) was estimated by EP parameters (latency, amplitude, and duration) of the first positive and negative components. It has been shown that the significance of the coefficients of correlation between EP parameters in the studied structures is determined by the stage of the behaviour formation; it also depends on the EP component chosen for the analysis. Significant correlation coefficients are timed to the active period in the formation of defensive conditioned reaction. In this period significant correlation coefficients for the parameters of the first positive EP component are established in various fields of cortical projection zones and between the parameters of the negative component in all relays of the analyzer. The data are treated from the viewpoint of adaptation of the analyser activity to the organism's needs at each stage in the formation adaptive behaviour and of the role of motivational influences in the organization of intraanalyzer integrations."}
{"id": "PMID:484026", "title": "[Effect of serotonin injected into the amygdaloid complex on conditioned and unconditioned alimentary reflexes and electrical brain activity in cats].", "content": "The effect of 5-OT injection into the basolateral area of the amygdala complex on conditioned and unconditioned alimentary reflexes and on the concomitant amygdala electrical activity was studied in cats. 5-OT in 100 to 200 mcg doses inhibited the reproduction of conditioned reflexes only, not affecting the unconditioned ones. Under the influence of 5-OT, generation of high-frequency burst activity by the amygdala was blocked; spatial synchronization between the visual and sensorimotor cortex, between the neocortex and the hippocampus was reduced. It is suggested that the basolateral area of the amygdala participates in the organization of conditioned reflexes, and that the serotoninergic system is involved in the neurochemical mechanisms of the inhibitory influence of this structure on the reproduction of conditioned reflexes.", "contents": "[Effect of serotonin injected into the amygdaloid complex on conditioned and unconditioned alimentary reflexes and electrical brain activity in cats]. The effect of 5-OT injection into the basolateral area of the amygdala complex on conditioned and unconditioned alimentary reflexes and on the concomitant amygdala electrical activity was studied in cats. 5-OT in 100 to 200 mcg doses inhibited the reproduction of conditioned reflexes only, not affecting the unconditioned ones. Under the influence of 5-OT, generation of high-frequency burst activity by the amygdala was blocked; spatial synchronization between the visual and sensorimotor cortex, between the neocortex and the hippocampus was reduced. It is suggested that the basolateral area of the amygdala participates in the organization of conditioned reflexes, and that the serotoninergic system is involved in the neurochemical mechanisms of the inhibitory influence of this structure on the reproduction of conditioned reflexes."}
{"id": "PMID:484027", "title": "[Restoration of instrumental movements in cats after section of spinal cord afferent pathways].", "content": "Instrumental fore-leg movements (pedal pressing) were studied in the course of recovery of motor functions after section of the posterior columns and the spino-cervical tract at the cervical level. Recovery of supporting-locomotor functions reached a high level. Instrumental reactions connected with inborn forms of motor activity (running etc.) were being restored parallel to the locomotion restoration. Complex instrumental movements were restored slower and didn't reach a high degree of accuracy. A more simple instrumental movement (pressing on the open pedal) had a more rapid and more complete recovery. It is concluded that the possibilities of substitution of different channels, transmitting afferent somatic impulses to the sensorimotor cortex, are very limited for elaborated instrumental movements.", "contents": "[Restoration of instrumental movements in cats after section of spinal cord afferent pathways]. Instrumental fore-leg movements (pedal pressing) were studied in the course of recovery of motor functions after section of the posterior columns and the spino-cervical tract at the cervical level. Recovery of supporting-locomotor functions reached a high level. Instrumental reactions connected with inborn forms of motor activity (running etc.) were being restored parallel to the locomotion restoration. Complex instrumental movements were restored slower and didn't reach a high degree of accuracy. A more simple instrumental movement (pressing on the open pedal) had a more rapid and more complete recovery. It is concluded that the possibilities of substitution of different channels, transmitting afferent somatic impulses to the sensorimotor cortex, are very limited for elaborated instrumental movements."}
{"id": "PMID:484028", "title": "[Conditioned reactions elaborated on the basis of reinforcement by hypothalamic stimulation].", "content": "The possibility of elaboration of conditioned emotional reactions on the basis of the stimulation of the hypothalamus, was studied in cats. Reinforcement of a sound (500 c/s) by electrical stimulation of the hypothalamus, which produced reactions of undirected rage, of fear with avoidance, of food search, led to the formation of a conditioned passive defensive reaction, analogous to that based on pain reinforcement. Elaboration of conditioned emotional reactions were blocked by switching off of the sensorimotor cortex by cold. A conclusion is made that during stimulation of the hypothalamus, the state of fear serves as the reinforcing factor and that the sensorimotor neocortical areas participate in the estimation of biological significance of the conditioned signal.", "contents": "[Conditioned reactions elaborated on the basis of reinforcement by hypothalamic stimulation]. The possibility of elaboration of conditioned emotional reactions on the basis of the stimulation of the hypothalamus, was studied in cats. Reinforcement of a sound (500 c/s) by electrical stimulation of the hypothalamus, which produced reactions of undirected rage, of fear with avoidance, of food search, led to the formation of a conditioned passive defensive reaction, analogous to that based on pain reinforcement. Elaboration of conditioned emotional reactions were blocked by switching off of the sensorimotor cortex by cold. A conclusion is made that during stimulation of the hypothalamus, the state of fear serves as the reinforcing factor and that the sensorimotor neocortical areas participate in the estimation of biological significance of the conditioned signal."}
{"id": "PMID:484029", "title": "[Conditioned reflex analog of sensomotor cortex neurons to acetylcholine microinjection].", "content": "Conditioned responses to time were studied in neurones of the sensorimotor cortex in alert rabbits using fixed time interval (2 min) combinations of sound stimuli with microionophoretic injection of acetylcholine in the recorded cell's area. Conditioned responses to time were elaborated in 26 out of 50 recorded cells (52%), both of activation (21 cells) and inhibitory (5 cells) types. They appeared after 10--30 pairings and were reproduced during 10--15 consequent omissions of the signal which points to persistence of the conditioned trace responses. It is assumed that the reinforcing effect of acetylcholine is basically due to the increased excitability of the neurone during application of the drug.", "contents": "[Conditioned reflex analog of sensomotor cortex neurons to acetylcholine microinjection]. Conditioned responses to time were studied in neurones of the sensorimotor cortex in alert rabbits using fixed time interval (2 min) combinations of sound stimuli with microionophoretic injection of acetylcholine in the recorded cell's area. Conditioned responses to time were elaborated in 26 out of 50 recorded cells (52%), both of activation (21 cells) and inhibitory (5 cells) types. They appeared after 10--30 pairings and were reproduced during 10--15 consequent omissions of the signal which points to persistence of the conditioned trace responses. It is assumed that the reinforcing effect of acetylcholine is basically due to the increased excitability of the neurone during application of the drug."}
{"id": "PMID:484030", "title": "[Correlation between rate of avoidance reaction elaboration and catecholamine levels in rat brain].", "content": "The dependence of parameters of elaboration of avoidance reaction in a shuttle box on the level of noradrenaline (NA) and dopamine (DA) in the brain was studied in 39 male rats of Wistar line with body weight of 200--250 g. The animals that on the first day of learning had different number of errors also showed different levels of brain NA and DA. A great number of errors correlated with a low level of DA in the whole brain and a high level of NA in cortical-striate brain area. It was shown that the errors characterized the process of learning itself and were not due to motor activation.", "contents": "[Correlation between rate of avoidance reaction elaboration and catecholamine levels in rat brain]. The dependence of parameters of elaboration of avoidance reaction in a shuttle box on the level of noradrenaline (NA) and dopamine (DA) in the brain was studied in 39 male rats of Wistar line with body weight of 200--250 g. The animals that on the first day of learning had different number of errors also showed different levels of brain NA and DA. A great number of errors correlated with a low level of DA in the whole brain and a high level of NA in cortical-striate brain area. It was shown that the errors characterized the process of learning itself and were not due to motor activation."}
{"id": "PMID:484031", "title": "[Role of the cholinergic and monoaminergic systems of the brain in dissociated learning].", "content": "Rats placed in an experimental chamber, were trained to perform two dissociated reactions; the one--in a normal state, the other--after administration of physostigmine. Then the animals were divided into 10 groups and in each group the performance of the elaborated conditioned reflexes was tested after administration of the substances influencing cholinergic and monoaminergic brain systems. It is shown that the dissociation state after physostigmine administration is due to the influence of this substance on the cholinergic brain system. The increase of biogenic amines content in the central nervous system leads to the appearance of dissociation after a smaller dose of anticholinesterase. This seems to reflect a modulating influence of monoaminergic brain systems on the cholinergic system during performance of the dissociated reactions.", "contents": "[Role of the cholinergic and monoaminergic systems of the brain in dissociated learning]. Rats placed in an experimental chamber, were trained to perform two dissociated reactions; the one--in a normal state, the other--after administration of physostigmine. Then the animals were divided into 10 groups and in each group the performance of the elaborated conditioned reflexes was tested after administration of the substances influencing cholinergic and monoaminergic brain systems. It is shown that the dissociation state after physostigmine administration is due to the influence of this substance on the cholinergic brain system. The increase of biogenic amines content in the central nervous system leads to the appearance of dissociation after a smaller dose of anticholinesterase. This seems to reflect a modulating influence of monoaminergic brain systems on the cholinergic system during performance of the dissociated reactions."}
{"id": "PMID:484032", "title": "[Effect of destruction of the amygdaloid complex on the cardiac and motor components of a conditioned defense reaction].", "content": "The effect of bilateral ablation of the amygdala complex basolateral part on heart and motor components of conditioned defensive reaction was studied in chronic experiments on rats. After 10 presentations of the tone during habituation procedure the elaboration of conditioned reaction in operated and control animals was achieved in one session (10 pairings of the tone with electrical pain stimulation in a minute). The analysis of the changes in the heart rate of amygdalectomized rats immediately after the elaboration revealed a shorter time of retention of the heart rate tachycardiac changes elicited by aversive stimulation. In tests one hour and 24 hours after the elaboration, the operated animals showed no conditioned bradycardiac reaction to the tone. This fact suggests a severe impairment of the conditioned emotional reaction of fear. The absence of the emotional component of the conditioned reaction seems to result in the disturbance of reproduction of its motor component.", "contents": "[Effect of destruction of the amygdaloid complex on the cardiac and motor components of a conditioned defense reaction]. The effect of bilateral ablation of the amygdala complex basolateral part on heart and motor components of conditioned defensive reaction was studied in chronic experiments on rats. After 10 presentations of the tone during habituation procedure the elaboration of conditioned reaction in operated and control animals was achieved in one session (10 pairings of the tone with electrical pain stimulation in a minute). The analysis of the changes in the heart rate of amygdalectomized rats immediately after the elaboration revealed a shorter time of retention of the heart rate tachycardiac changes elicited by aversive stimulation. In tests one hour and 24 hours after the elaboration, the operated animals showed no conditioned bradycardiac reaction to the tone. This fact suggests a severe impairment of the conditioned emotional reaction of fear. The absence of the emotional component of the conditioned reaction seems to result in the disturbance of reproduction of its motor component."}
{"id": "PMID:484033", "title": "[Correlation between the retention of defensive conditioned reflexes and rat brain acetylcholinesterase activity].", "content": "The comparison of the characteristics of temporary connection retention (repeated elaboration) with the activity of acetylcholinesterase (AChE) of the motor, visual cerebral cortical areas and hippocampus was done at different time (1, 2 and 4 weeks) following elaboration of defensive conditioned reflexes (CR) in rats in a chamber of two-way avoidance with electrified floor. The maximal retention of CR was observed 2 weeks after the initial elaboration. The dynamics of AChE activity on the whole doesn't correspond to the changes in CR retention. Pronounced AChE activity decrease in the motor and visual cortex was observed during CR reproduction 4 weeks after elaboration. Significant differences by this parameter were observed between trained animals and the rats, presented with non-paired photic and electrical stimuli (active control).", "contents": "[Correlation between the retention of defensive conditioned reflexes and rat brain acetylcholinesterase activity]. The comparison of the characteristics of temporary connection retention (repeated elaboration) with the activity of acetylcholinesterase (AChE) of the motor, visual cerebral cortical areas and hippocampus was done at different time (1, 2 and 4 weeks) following elaboration of defensive conditioned reflexes (CR) in rats in a chamber of two-way avoidance with electrified floor. The maximal retention of CR was observed 2 weeks after the initial elaboration. The dynamics of AChE activity on the whole doesn't correspond to the changes in CR retention. Pronounced AChE activity decrease in the motor and visual cortex was observed during CR reproduction 4 weeks after elaboration. Significant differences by this parameter were observed between trained animals and the rats, presented with non-paired photic and electrical stimuli (active control)."}
{"id": "PMID:484034", "title": "[Effect of ablation of the basolateral and corticomedial portions of the amygdala on the performance of food-getting conditioned reflexes in rats].", "content": "In experiments on 66 albino rats an electrolytic coagulation of the basolateral amygdala caused facilitation while that of the corticomedial amygdala caused inhibition of alimentary conditioned reflexes. The corticomedial amygdala forms a part of the excitatory and the basolateral amygdala--of the inhibitory system of the rat's brain. In intact rats the basolateral amygdala function dominates over the corticomedial amygdala activity in feeding behaviour.", "contents": "[Effect of ablation of the basolateral and corticomedial portions of the amygdala on the performance of food-getting conditioned reflexes in rats]. In experiments on 66 albino rats an electrolytic coagulation of the basolateral amygdala caused facilitation while that of the corticomedial amygdala caused inhibition of alimentary conditioned reflexes. The corticomedial amygdala forms a part of the excitatory and the basolateral amygdala--of the inhibitory system of the rat's brain. In intact rats the basolateral amygdala function dominates over the corticomedial amygdala activity in feeding behaviour."}
{"id": "PMID:484035", "title": "[Conditioned reflex to time in grape snails].", "content": "Tactile stimulation of the snail's mantle evokes unconditioned avoidance reflex of pneumostome closure. After 3--4 experimental sessions (10 stimuli per session, a session per day) of rhythmical stimulation (interstimulus intervals 1, 2, 4 min) with some stimuli missed, the pneumostome closure occurs at the time when the missed stimulus had to be delivered, i. e. expectancy learning takes place. Arhythmical stimulation does not produce such a result. When the stimulation is stopped only two or three conditioned responses are recorded.", "contents": "[Conditioned reflex to time in grape snails]. Tactile stimulation of the snail's mantle evokes unconditioned avoidance reflex of pneumostome closure. After 3--4 experimental sessions (10 stimuli per session, a session per day) of rhythmical stimulation (interstimulus intervals 1, 2, 4 min) with some stimuli missed, the pneumostome closure occurs at the time when the missed stimulus had to be delivered, i. e. expectancy learning takes place. Arhythmical stimulation does not produce such a result. When the stimulation is stopped only two or three conditioned responses are recorded."}
{"id": "PMID:484036", "title": "[Formation of a conditioned motor food-getting reaction with a two-way connection in grape snails].", "content": "Tactile stimulation of the snail's skin normally evokes avoidance reactions; however after 150--300 reinforcements by food placed in a definite spot, this stimulus began to elicit food-searching reactions of the animal with its turning towards usual food position. The conditioned reflex was stable and reappeared spontaneously following active extinction. It could be recorded 6 weeks after the end of the training. Presentation of food evoked avoidance reactions in 35--60% of cases, but this feedback connection appeared irregularly.", "contents": "[Formation of a conditioned motor food-getting reaction with a two-way connection in grape snails]. Tactile stimulation of the snail's skin normally evokes avoidance reactions; however after 150--300 reinforcements by food placed in a definite spot, this stimulus began to elicit food-searching reactions of the animal with its turning towards usual food position. The conditioned reflex was stable and reappeared spontaneously following active extinction. It could be recorded 6 weeks after the end of the training. Presentation of food evoked avoidance reactions in 35--60% of cases, but this feedback connection appeared irregularly."}
{"id": "PMID:484037", "title": "[Relationship between the slow potentials of the human EEG, the cutaneous galvanic potential and the electrooculogram].", "content": "The paper deals with the possibilities of cross-correlational analysis as a control to revealing slow components of non-brain origin in human EEG, superimposed on the brain potentials. Such control is necessary in the study of low-frequency (2 c/s and lower) EEG manifestations (\"expectancy waves\", shifts in the level of the constant potential etc.). It is shown that EEG may include as slow components electrooculogram and SGR, both peripheral (hand) and that of the scalp (temporal and frontal). The share of the responses depends on the functional state of the healthy subject.", "contents": "[Relationship between the slow potentials of the human EEG, the cutaneous galvanic potential and the electrooculogram]. The paper deals with the possibilities of cross-correlational analysis as a control to revealing slow components of non-brain origin in human EEG, superimposed on the brain potentials. Such control is necessary in the study of low-frequency (2 c/s and lower) EEG manifestations (\"expectancy waves\", shifts in the level of the constant potential etc.). It is shown that EEG may include as slow components electrooculogram and SGR, both peripheral (hand) and that of the scalp (temporal and frontal). The share of the responses depends on the functional state of the healthy subject."}
{"id": "PMID:484038", "title": "[Readiness potential during tracing movements].", "content": "Readiness potential (RP) has been defined in EEG recorded in the sensorimotor human cortex during tracing movements. RPs which appear prior to the tracing of different programmes, differ in the time of their appearance, duration and amplitude. Dependence has been established between the RP amplitude and tracing movements latency: movements latency is lower at higher RP amplitudes, and vice versa.", "contents": "[Readiness potential during tracing movements]. Readiness potential (RP) has been defined in EEG recorded in the sensorimotor human cortex during tracing movements. RPs which appear prior to the tracing of different programmes, differ in the time of their appearance, duration and amplitude. Dependence has been established between the RP amplitude and tracing movements latency: movements latency is lower at higher RP amplitudes, and vice versa."}
{"id": "PMID:484039", "title": "[Effect of catecholamines on the motivational and reinforcing effects of self stimulation zones].", "content": "A study has been made of the influence of disulphiram--a drug that lowers the level of norepinephrine and DOPA which restores its content in brain tissues,--on motivational and reinforcing effects caused by the action of a gradually increasing current on the self-stimulation (SS) zones. It has been shown that disulphiram, while suppressing motivational reactions does not exert such an effect on SS reactions. A half of the studied animals showed in these conditions an increased SS frequency. During disulphiram action (on the second day) DOPA restored motivational reactions and inhibited SS reactions. It is assumed that norepinephrine is involved in the mediatory system activating goal-directed motivational behaviour, but it does not constitute, as it seems, the major mediator of the reinforcement system.", "contents": "[Effect of catecholamines on the motivational and reinforcing effects of self stimulation zones]. A study has been made of the influence of disulphiram--a drug that lowers the level of norepinephrine and DOPA which restores its content in brain tissues,--on motivational and reinforcing effects caused by the action of a gradually increasing current on the self-stimulation (SS) zones. It has been shown that disulphiram, while suppressing motivational reactions does not exert such an effect on SS reactions. A half of the studied animals showed in these conditions an increased SS frequency. During disulphiram action (on the second day) DOPA restored motivational reactions and inhibited SS reactions. It is assumed that norepinephrine is involved in the mediatory system activating goal-directed motivational behaviour, but it does not constitute, as it seems, the major mediator of the reinforcement system."}
{"id": "PMID:484040", "title": "[Character of functional connections between sensomotor cortex neurons during isolated and combined stimulation of thalamic nuclei].", "content": "In experiments on curarized cats pronounced transformations of the \"excitatory\" component of the \"inhibitory-excitatory\" dependence of two close neurones in the sensorimotor cortex were found following multiple isolated VPL and VL stimulation. The effects of repetitive stimulation of thalamic nuclei were opposite to each other: VPL stimulation mainly weakened the \"excitatory\" component of the \"inhibitory-excitatory\" connection, whereas VL stimulation generally enhanced it. In the course of combined stimulations of both nuclei the character of interneuronal relations, established under the influence of isolated stimuli, showed further changes. Modifications of interneuronal dependence, resulting from thalamic stimulation, could last for several minutes after cessation of all stimulations.", "contents": "[Character of functional connections between sensomotor cortex neurons during isolated and combined stimulation of thalamic nuclei]. In experiments on curarized cats pronounced transformations of the \"excitatory\" component of the \"inhibitory-excitatory\" dependence of two close neurones in the sensorimotor cortex were found following multiple isolated VPL and VL stimulation. The effects of repetitive stimulation of thalamic nuclei were opposite to each other: VPL stimulation mainly weakened the \"excitatory\" component of the \"inhibitory-excitatory\" connection, whereas VL stimulation generally enhanced it. In the course of combined stimulations of both nuclei the character of interneuronal relations, established under the influence of isolated stimuli, showed further changes. Modifications of interneuronal dependence, resulting from thalamic stimulation, could last for several minutes after cessation of all stimulations."}
{"id": "PMID:484041", "title": "[Effect of coagulation of the reticular formation in the region of the NRP and NRT nuclei on the spread of brain biopotential synchronization in rabbits].", "content": "The influence of coagulation of NRP and NRT nuclei on the level of spatial synchronization of bioelectrical activity in the visual and motor cortical areas was studied in chronic experiments on alert rabbits. It was shown that elimination of these structures from activity diminishes the correlation of processes in the studied zones. The degree of the coagulation effect depends on the volume of destruction and on the degree of synchroneity of the background neocortical biopotentials. Destruction of the nuclei is attended with dominance of delta-frequencies in the power spectra and a drop of coherence function in the theta range. It is assumed that the NRP and NRT nuclei are functional links of the integral system which controls the level of spatial synchronization of bioelectrical processes in the rabbit cerebral cortex.", "contents": "[Effect of coagulation of the reticular formation in the region of the NRP and NRT nuclei on the spread of brain biopotential synchronization in rabbits]. The influence of coagulation of NRP and NRT nuclei on the level of spatial synchronization of bioelectrical activity in the visual and motor cortical areas was studied in chronic experiments on alert rabbits. It was shown that elimination of these structures from activity diminishes the correlation of processes in the studied zones. The degree of the coagulation effect depends on the volume of destruction and on the degree of synchroneity of the background neocortical biopotentials. Destruction of the nuclei is attended with dominance of delta-frequencies in the power spectra and a drop of coherence function in the theta range. It is assumed that the NRP and NRT nuclei are functional links of the integral system which controls the level of spatial synchronization of bioelectrical processes in the rabbit cerebral cortex."}
{"id": "PMID:484054", "title": "The amylase-producing microflora of semi-preserved canned sausages: identification of the bacteria and characterization of their amylases.", "content": "Thirteen strains of amylase-producing bacteria were isolated from semi-preserved canned sausages and their ingredients. All belonged to the genus Bacillus, and could be separated into 4 different groups. Two groups were different strain of B. subtilis, one was B. amyloliquefaciens and the last was B. macerans. The identification of the different bacteria species was supported by disc gel electrophoresis of the supernatant culture fluid, after growth. The amylases were characterized with regard to temperature optimum, pH optimum and thermostability. Although some of the amylases appear to be quite thermostable, the only explanation for starch degradation in semi-preserved foods seems to be the amylase production from outgrowing spores which survived the heat treatment.", "contents": "The amylase-producing microflora of semi-preserved canned sausages: identification of the bacteria and characterization of their amylases. Thirteen strains of amylase-producing bacteria were isolated from semi-preserved canned sausages and their ingredients. All belonged to the genus Bacillus, and could be separated into 4 different groups. Two groups were different strain of B. subtilis, one was B. amyloliquefaciens and the last was B. macerans. The identification of the different bacteria species was supported by disc gel electrophoresis of the supernatant culture fluid, after growth. The amylases were characterized with regard to temperature optimum, pH optimum and thermostability. Although some of the amylases appear to be quite thermostable, the only explanation for starch degradation in semi-preserved foods seems to be the amylase production from outgrowing spores which survived the heat treatment."}
{"id": "PMID:484055", "title": "[Somatic parameters in patients with urinary calculi and their relationship to age, sex and kind of calculus].", "content": "In the present investigation in 515 carriers of nephroliths (311 males and 204 females) age, sex, type of constitution, body weight, physical load and blood pressure are set in relation to the various kinds of calculi. Here it was revealed that males more frequently fall ill with nephrolithiasis than females, that there exist certain age peaks, the pycnic typ of constitution preferredly appears in female carriers of calculi and the athletic type in male ones, the majority of all examined patients had in comparison to the normal an increased body weight, and a very large proportion of persons with only insignificant physical load is found among the carriers of calculi. The result of the relations of blood pressure was that only half of all patients with urolithiasis examined had a normotonic blood pressure.", "contents": "[Somatic parameters in patients with urinary calculi and their relationship to age, sex and kind of calculus]. In the present investigation in 515 carriers of nephroliths (311 males and 204 females) age, sex, type of constitution, body weight, physical load and blood pressure are set in relation to the various kinds of calculi. Here it was revealed that males more frequently fall ill with nephrolithiasis than females, that there exist certain age peaks, the pycnic typ of constitution preferredly appears in female carriers of calculi and the athletic type in male ones, the majority of all examined patients had in comparison to the normal an increased body weight, and a very large proportion of persons with only insignificant physical load is found among the carriers of calculi. The result of the relations of blood pressure was that only half of all patients with urolithiasis examined had a normotonic blood pressure."}
{"id": "PMID:484056", "title": "[A modified method for the quantitative determination of urinary oxalic acid].", "content": "The present paper describes a modified method of the quantitative determination of oxalic acid in the urine which is based on the reduction of oxalic acid to glycolic acid and on the transformation with chromotropic acid to a colorimetrically well measurable complex of colours. The method ascertains a good reproducibility and is simply to be performed in every clinico-chemical laboratory.", "contents": "[A modified method for the quantitative determination of urinary oxalic acid]. The present paper describes a modified method of the quantitative determination of oxalic acid in the urine which is based on the reduction of oxalic acid to glycolic acid and on the transformation with chromotropic acid to a colorimetrically well measurable complex of colours. The method ascertains a good reproducibility and is simply to be performed in every clinico-chemical laboratory."}
{"id": "PMID:484057", "title": "[Indication for operation in acute kidney obstruction caused by urinary calculi].", "content": "We like to discuss the comment of our clinic: In case of an acute stone-conditioned renal blockage complicated by fever we immediately perform a decompression by means of elevation of the ureter catheter and the antibiotic therapy. A shivering attack accompanied by fever we regard as a threatening sign of pyelonephritis apostematosa and possibly after the first or second shivering attack we perform an opening. Apart from emphasizing the importance of the shivering attack we still might refer to the fact that for the establishment of the therapy of choice the complex evaluation of the clinical picture is necessary.", "contents": "[Indication for operation in acute kidney obstruction caused by urinary calculi]. We like to discuss the comment of our clinic: In case of an acute stone-conditioned renal blockage complicated by fever we immediately perform a decompression by means of elevation of the ureter catheter and the antibiotic therapy. A shivering attack accompanied by fever we regard as a threatening sign of pyelonephritis apostematosa and possibly after the first or second shivering attack we perform an opening. Apart from emphasizing the importance of the shivering attack we still might refer to the fact that for the establishment of the therapy of choice the complex evaluation of the clinical picture is necessary."}
{"id": "PMID:484058", "title": "[Experiences with new methods for the preoperative localization and surgical removal of complicated kidney calculi].", "content": "In a survey is described the inpatients suffering from urolithiasis of the urological clinic Berlin-Friedrichshain of the last three years from 1 April 1975 to 31 March 1978. Especially the cast calculi of the renal calyx were analysed and the therapeutic approach was discussed. For the complete removal of coral calculi in our clinic preoperatively made tomographies and adequate graphs of the cast concrement in its relation to the hollow system at two levels also stood the test as the intraoperative use of the X-ray device \"Renodor\". The two methods were described in detail. Also in multiple and movable concrements in the system of the renal calyx the Renodor stood the test. Here we could make good experiences also with the fibrin pyelolithothomy--the indications and results of these methods are represented.", "contents": "[Experiences with new methods for the preoperative localization and surgical removal of complicated kidney calculi]. In a survey is described the inpatients suffering from urolithiasis of the urological clinic Berlin-Friedrichshain of the last three years from 1 April 1975 to 31 March 1978. Especially the cast calculi of the renal calyx were analysed and the therapeutic approach was discussed. For the complete removal of coral calculi in our clinic preoperatively made tomographies and adequate graphs of the cast concrement in its relation to the hollow system at two levels also stood the test as the intraoperative use of the X-ray device \"Renodor\". The two methods were described in detail. Also in multiple and movable concrements in the system of the renal calyx the Renodor stood the test. Here we could make good experiences also with the fibrin pyelolithothomy--the indications and results of these methods are represented."}
{"id": "PMID:484059", "title": "[Pseudomorphoses in calcium oxalate urinary calculi].", "content": "Pseudomorphoses of whewellite to weddellite reflect on transparent section preparations and in the REM appearances of cristallisation processes in the secondary whewellite formation via weddellite dehydration. As transformation products are found fine-grained polycrystalline as well as due to recrystallisation larger monocrystalline whewellite. An interesting analogy to this gives the thermic dehydration of weddellite crystals in direction to whewellite, which realises under formation of pseudomorphoses as topotactic process. The dissociation scars appearing in this case are strongly oriented to the crystal grid of weddellite.", "contents": "[Pseudomorphoses in calcium oxalate urinary calculi]. Pseudomorphoses of whewellite to weddellite reflect on transparent section preparations and in the REM appearances of cristallisation processes in the secondary whewellite formation via weddellite dehydration. As transformation products are found fine-grained polycrystalline as well as due to recrystallisation larger monocrystalline whewellite. An interesting analogy to this gives the thermic dehydration of weddellite crystals in direction to whewellite, which realises under formation of pseudomorphoses as topotactic process. The dissociation scars appearing in this case are strongly oriented to the crystal grid of weddellite."}
{"id": "PMID:484060", "title": "[Results of follow-up studies in children with megaureters].", "content": "It is reported on the results of after-examinations in children with megaureters. The average interval between first operation and last after-examination is 8.1 years (1 to 14 years). Of altogether 63 children ten died, eight of their disease, including the postoperative complications. Eleven children were nephroureterectomized. In spite of the time of satisfying results concerning the renal function the prognosis is uncertain, particularly of the children with bilateral malformation. The number of the infections of the urinary tract is large and the deterioration of the function due to pyelonephritis is not to be foreseen. Changes or modifications of the operation technique and a strict dispensary care of the children let us hope for an improvement of the results during the next years.", "contents": "[Results of follow-up studies in children with megaureters]. It is reported on the results of after-examinations in children with megaureters. The average interval between first operation and last after-examination is 8.1 years (1 to 14 years). Of altogether 63 children ten died, eight of their disease, including the postoperative complications. Eleven children were nephroureterectomized. In spite of the time of satisfying results concerning the renal function the prognosis is uncertain, particularly of the children with bilateral malformation. The number of the infections of the urinary tract is large and the deterioration of the function due to pyelonephritis is not to be foreseen. Changes or modifications of the operation technique and a strict dispensary care of the children let us hope for an improvement of the results during the next years."}
{"id": "PMID:484061", "title": "[The long-term effect of various hormonal contraceptives on the excretion of sodium, potassium, calcium, magnesium, chloride, phosphorus, uric acid, oxalic acid, citric acid, sulfate and lysozyme in the 24 hour urine].", "content": "The influence of a long-term application of 4 different hormonal contraceptives on the excretion of various constituents of the urine was examined. A reverse effect after stoppage of the medication is to be established in none of the other parameters with the exception of calcium.", "contents": "[The long-term effect of various hormonal contraceptives on the excretion of sodium, potassium, calcium, magnesium, chloride, phosphorus, uric acid, oxalic acid, citric acid, sulfate and lysozyme in the 24 hour urine]. The influence of a long-term application of 4 different hormonal contraceptives on the excretion of various constituents of the urine was examined. A reverse effect after stoppage of the medication is to be established in none of the other parameters with the exception of calcium."}
{"id": "PMID:484062", "title": "[Effect of sex steroids on the rat testicular blood supply].", "content": "The effect of oestradiolundecilate and testosterone oenanthate on the blood supply of the testicle of rats was investigated by means of the Xe-133 tissue clearance. The investigation shows the following results: 1. Oestradiolundecilate leads to a highly significant decrease of the blood supply of the testicles. 2. The effect of testosterone oenanthate depended upon the dosage. 0.3 mg three times a week led to a reduction of the blood supply, whereas below 3.0 mg three times a week no difference of the values of blood supply appeared in comparison to the initial values. Connections between the functional changes evoked by the application of the sexual steroids and the behaviour of the blood supply are discussed.", "contents": "[Effect of sex steroids on the rat testicular blood supply]. The effect of oestradiolundecilate and testosterone oenanthate on the blood supply of the testicle of rats was investigated by means of the Xe-133 tissue clearance. The investigation shows the following results: 1. Oestradiolundecilate leads to a highly significant decrease of the blood supply of the testicles. 2. The effect of testosterone oenanthate depended upon the dosage. 0.3 mg three times a week led to a reduction of the blood supply, whereas below 3.0 mg three times a week no difference of the values of blood supply appeared in comparison to the initial values. Connections between the functional changes evoked by the application of the sexual steroids and the behaviour of the blood supply are discussed."}
{"id": "PMID:484063", "title": "[Kidney calculi and medullary neoplasms].", "content": "Even though we do not find confirmed among our patients the high percentage of 25% of kidneys with medullary carcinoma in lithogenetic patients - this is certainly connected with the fact that our patients are in no way selected - we nevertheless are of the opinion that this picture of a disease should more considered and because of the danger of progression the possibilities of a preventive therapy which are at present at our disposal should increasedly be used.", "contents": "[Kidney calculi and medullary neoplasms]. Even though we do not find confirmed among our patients the high percentage of 25% of kidneys with medullary carcinoma in lithogenetic patients - this is certainly connected with the fact that our patients are in no way selected - we nevertheless are of the opinion that this picture of a disease should more considered and because of the danger of progression the possibilities of a preventive therapy which are at present at our disposal should increasedly be used."}
{"id": "PMID:484065", "title": "[Chronic sodium chloride load--a possibility for the increase of tolerance for ischemia in the kidney].", "content": "After an ischaemia lasting 1 hour as well as after an extreme hypertension lasting 210 minutes in the kidney of a rat a significant increase of the renin activity in the juxtaglomerular apparatus was found. By chronic load with sodium chloride was tried to produce a decrease of renin. In contrast to an ischaemic lesion the kidneys of the animals loaded with salt revealed a better ischaemia tolerance than those of the unloaded animals. This is expressed by a significantly increased total blood supply and cortical blood supply. On the two experimental conditions a change of the distribution of the blood supply in favour of the inner compartments of the kidneys may be observed. In the oxygen histogram of the surface of the kidney the salt-loaded kidneys reveal a better oxygenation before and after the ischaemic lesion. The kidneys loaded before show an essentially more insignificant decrease of the urine excretion than the unloaded ones.", "contents": "[Chronic sodium chloride load--a possibility for the increase of tolerance for ischemia in the kidney]. After an ischaemia lasting 1 hour as well as after an extreme hypertension lasting 210 minutes in the kidney of a rat a significant increase of the renin activity in the juxtaglomerular apparatus was found. By chronic load with sodium chloride was tried to produce a decrease of renin. In contrast to an ischaemic lesion the kidneys of the animals loaded with salt revealed a better ischaemia tolerance than those of the unloaded animals. This is expressed by a significantly increased total blood supply and cortical blood supply. On the two experimental conditions a change of the distribution of the blood supply in favour of the inner compartments of the kidneys may be observed. In the oxygen histogram of the surface of the kidney the salt-loaded kidneys reveal a better oxygenation before and after the ischaemic lesion. The kidneys loaded before show an essentially more insignificant decrease of the urine excretion than the unloaded ones."}
{"id": "PMID:484066", "title": "[Value of the electrophoresis mobility test in the differential diagnosis of obscure kidney findings].", "content": "The differential diagnosis of unclear carcinoma-suspicious renal findings often finishes with the test-exposition and nephrectomy. Also when all modern diagnostic possibilities are used not always a clear establishment of the diagnosis may be achieved. The cell electrophoresis mobility test after Field and Caspary may contribute to the differential-diagnostic clarification of the findings, when adequate antigens are used. For this purpose 10 own cases are discussed and these results are compared with suitable comparative groups. Summarizingly by the present study can be shown that the immunologic diagnostics of tumours in the field of unclear renal tumours may be a very good help in the diagnostics. When adequate antigens are used in the cell electrophoresis mobility test a coordination concerning the histospecificity can be achieved.", "contents": "[Value of the electrophoresis mobility test in the differential diagnosis of obscure kidney findings]. The differential diagnosis of unclear carcinoma-suspicious renal findings often finishes with the test-exposition and nephrectomy. Also when all modern diagnostic possibilities are used not always a clear establishment of the diagnosis may be achieved. The cell electrophoresis mobility test after Field and Caspary may contribute to the differential-diagnostic clarification of the findings, when adequate antigens are used. For this purpose 10 own cases are discussed and these results are compared with suitable comparative groups. Summarizingly by the present study can be shown that the immunologic diagnostics of tumours in the field of unclear renal tumours may be a very good help in the diagnostics. When adequate antigens are used in the cell electrophoresis mobility test a coordination concerning the histospecificity can be achieved."}
{"id": "PMID:484067", "title": "[A contribution to bladder amyloidosis].", "content": "It is reported on a localised primary amyolidosis of the urinary bladder with a progressing course. In contrast to the prevailing observations of other authors during two years an increasing bilateral urinary stasis developed so that finally after an at first unilateral nephrostomy due to an infected retention kidney the bilateral ureter-intestine-anastomosis became necessary. Apart from a short description of the amyloidosis from the general and especially urologi-al point of view it is particularly referred to diagnostic difficulties, since the various forms of amyloidosis have a different prognosis and demand a therapy.", "contents": "[A contribution to bladder amyloidosis]. It is reported on a localised primary amyolidosis of the urinary bladder with a progressing course. In contrast to the prevailing observations of other authors during two years an increasing bilateral urinary stasis developed so that finally after an at first unilateral nephrostomy due to an infected retention kidney the bilateral ureter-intestine-anastomosis became necessary. Apart from a short description of the amyloidosis from the general and especially urologi-al point of view it is particularly referred to diagnostic difficulties, since the various forms of amyloidosis have a different prognosis and demand a therapy."}
{"id": "PMID:484082", "title": "[Epidemiology of burn injuries in the GDR (author's transl)].", "content": "Burn injuries in GDR have a slight decreasing tendency concerning both morbidity and mortality. The distribution of injuries to the districts of GDR can be explained by the distribution of population, not by distribution of industry. The overwhelming share of injuries consists of children with small scalds. Therapeutical results are comparable with results from international literature. Owing to the fact that in last years no further decisive improvement could be achieved, more attention must be devoted to the problem of primary prevention especially to target groups, resulting from anamnestic and clinical data analysis.", "contents": "[Epidemiology of burn injuries in the GDR (author's transl)]. Burn injuries in GDR have a slight decreasing tendency concerning both morbidity and mortality. The distribution of injuries to the districts of GDR can be explained by the distribution of population, not by distribution of industry. The overwhelming share of injuries consists of children with small scalds. Therapeutical results are comparable with results from international literature. Owing to the fact that in last years no further decisive improvement could be achieved, more attention must be devoted to the problem of primary prevention especially to target groups, resulting from anamnestic and clinical data analysis."}
{"id": "PMID:484083", "title": "[Prophylaxis and treatment of infectious complications in burns (author's transl)].", "content": "To lower infectious complications and the mortality rate due to septicaemia in burned patients more knowledge should be acquired to increase the natural resistance of the organism of the burned. This can be done by administering Staphylococcal-Anatoxin, Antistaphylococcal-Plasma, Antipyocyaneus-Serum and convalescent-Serum.", "contents": "[Prophylaxis and treatment of infectious complications in burns (author's transl)]. To lower infectious complications and the mortality rate due to septicaemia in burned patients more knowledge should be acquired to increase the natural resistance of the organism of the burned. This can be done by administering Staphylococcal-Anatoxin, Antistaphylococcal-Plasma, Antipyocyaneus-Serum and convalescent-Serum."}
{"id": "PMID:484084", "title": "[Arthrodesis of the knee joint (author's transl)].", "content": "Arthrodesis of the knee joint was performed in 75 patients. Solid fusion was achieved in 74 out of them. Average time for union was basically dependent upon type of pathological process and not upon fusion technique employed. In the last years compression arthrodesis with internal fixation by plates was used in all cases of degenerative, posttraumatic and rheumatoid arthrosis; in cases of tuberculosis and other infections of the knee joint external osteosynthesis by fixateur externe was the preferred procedure.", "contents": "[Arthrodesis of the knee joint (author's transl)]. Arthrodesis of the knee joint was performed in 75 patients. Solid fusion was achieved in 74 out of them. Average time for union was basically dependent upon type of pathological process and not upon fusion technique employed. In the last years compression arthrodesis with internal fixation by plates was used in all cases of degenerative, posttraumatic and rheumatoid arthrosis; in cases of tuberculosis and other infections of the knee joint external osteosynthesis by fixateur externe was the preferred procedure."}
{"id": "PMID:484087", "title": "[Theoretical and methodical aspects of sociogynaecology (author's transl)].", "content": "The objectives and purpose of sociogynaecology are defined. Included in it are the following lines of research: (a) Health care for women; (b) Coordination, planning, and organisation of appropriate health services: (c) Social factors of relevance to health care for women: (d) Health education of women.", "contents": "[Theoretical and methodical aspects of sociogynaecology (author's transl)]. The objectives and purpose of sociogynaecology are defined. Included in it are the following lines of research: (a) Health care for women; (b) Coordination, planning, and organisation of appropriate health services: (c) Social factors of relevance to health care for women: (d) Health education of women."}
{"id": "PMID:484088", "title": "[Efficient organisational structures of in-patient and out-patient practice for satisfactory gynecologico-obstetric attention (author's transl)].", "content": "The strong need for integrated management of gynaecologico-obstetric attention as well as the role played by interdisciplinary cooperation as a prerequisite for high-continuity medico-social services are expounded against the background of health policies established for the current period up to 1980 and with close reference to experience obtained in every-day practice. The introduction of demand-oriented organisational forms by which to enhance the standards and effectiveness of health care for mother and child is essential to the general practice of sociogynaecology.", "contents": "[Efficient organisational structures of in-patient and out-patient practice for satisfactory gynecologico-obstetric attention (author's transl)]. The strong need for integrated management of gynaecologico-obstetric attention as well as the role played by interdisciplinary cooperation as a prerequisite for high-continuity medico-social services are expounded against the background of health policies established for the current period up to 1980 and with close reference to experience obtained in every-day practice. The introduction of demand-oriented organisational forms by which to enhance the standards and effectiveness of health care for mother and child is essential to the general practice of sociogynaecology."}
{"id": "PMID:484089", "title": "[Methodical problems in assessment of fertility development (author's transl)].", "content": "Birth control as well as medical attention to and counselling of women in the context of pregnancy, childbirth, development of fertility, and reproduction of population may be considered as major lines of sociogynaecology. An attempt is made in this paper to provide insights into methodical aspects with relevance to assessment and prognostication of those processes. Correlations are expounded between possibilities of birth control and fertility development, and reference is made to controlled variation of birth dates within the fertile phase of women together with the impact of such variation of fertility.", "contents": "[Methodical problems in assessment of fertility development (author's transl)]. Birth control as well as medical attention to and counselling of women in the context of pregnancy, childbirth, development of fertility, and reproduction of population may be considered as major lines of sociogynaecology. An attempt is made in this paper to provide insights into methodical aspects with relevance to assessment and prognostication of those processes. Correlations are expounded between possibilities of birth control and fertility development, and reference is made to controlled variation of birth dates within the fertile phase of women together with the impact of such variation of fertility."}
{"id": "PMID:484090", "title": "[Possible assessment of standards and efficiency of ambulatory gynaecological services as part of sociogynaecology (author's transl)].", "content": "There are no criteria, as yet, by which to measure the standards of gynaecological outpatient practice. Specific criteria should be devised for an assessment of efficient and service quality. The detection of carcinoma in situ so far has differed from region to region. The degree of detection, therefore, is believed to be a good measure of efficiency. Some general aspects of quality assessment are discussed. Work on such assessment of standards and efficiency is imperative in sociogynaecology both for a general enhancement of methods and the solution of problems relating to management, planning, and organisation of public health at large.", "contents": "[Possible assessment of standards and efficiency of ambulatory gynaecological services as part of sociogynaecology (author's transl)]. There are no criteria, as yet, by which to measure the standards of gynaecological outpatient practice. Specific criteria should be devised for an assessment of efficient and service quality. The detection of carcinoma in situ so far has differed from region to region. The degree of detection, therefore, is believed to be a good measure of efficiency. Some general aspects of quality assessment are discussed. Work on such assessment of standards and efficiency is imperative in sociogynaecology both for a general enhancement of methods and the solution of problems relating to management, planning, and organisation of public health at large."}
{"id": "PMID:484091", "title": "[Demographic and sociological setup of premature deliveries for live births in GDR 1973 (author's transl)].", "content": "A record was taken 1973 of 22,466 single and 3,287 multiple live births, with premature deliveries being subdivided by age, birth order, coverture, and vocational backgrounds of the mothers concerned. The distributions thus established have produced evidence to the effect that all the above factors may have an impact upon premature delivery. Yet, multiple births were found to distort the distributions of all parameters used. --- Reference is made to possible interpretations of such studies in terms of large-scale trends, resources, risk hypotheses, and clusters, with examples being proposed for the latter.", "contents": "[Demographic and sociological setup of premature deliveries for live births in GDR 1973 (author's transl)]. A record was taken 1973 of 22,466 single and 3,287 multiple live births, with premature deliveries being subdivided by age, birth order, coverture, and vocational backgrounds of the mothers concerned. The distributions thus established have produced evidence to the effect that all the above factors may have an impact upon premature delivery. Yet, multiple births were found to distort the distributions of all parameters used. --- Reference is made to possible interpretations of such studies in terms of large-scale trends, resources, risk hypotheses, and clusters, with examples being proposed for the latter."}
{"id": "PMID:484092", "title": "[Methodical aspects and results of studies into motivations for induced termination of pregnancy and the wish to have a child (author's transl)].", "content": "Medical and social aspects relating to human reproduction has been the heading of a research subject in the context of sociogynaecology. An account is given in this paper of medicosociological studies and survey approaches with regard to induced-termination patients, puerperae and a control population, with emphasis being laid on identifying attitudes, motivations, and general views on having children. -- Methodical aspects are reported and assessed together with preliminary results obtained from a GDR-representative sub-study into 2,776 induced-termination patients. Priorities issues included the social situation of the interviewees, their attitudes towards having a child and to induced termination of pregnancy, reasons given for termination decisions, as well as views on contraception and family planning.", "contents": "[Methodical aspects and results of studies into motivations for induced termination of pregnancy and the wish to have a child (author's transl)]. Medical and social aspects relating to human reproduction has been the heading of a research subject in the context of sociogynaecology. An account is given in this paper of medicosociological studies and survey approaches with regard to induced-termination patients, puerperae and a control population, with emphasis being laid on identifying attitudes, motivations, and general views on having children. -- Methodical aspects are reported and assessed together with preliminary results obtained from a GDR-representative sub-study into 2,776 induced-termination patients. Priorities issues included the social situation of the interviewees, their attitudes towards having a child and to induced termination of pregnancy, reasons given for termination decisions, as well as views on contraception and family planning."}
{"id": "PMID:484094", "title": "[Cardiotocography -- proposal of system solution (author's transl)].", "content": "A system solution with new technical details and new demands on space distribution is proposed for the use of antenatal cardiotocography and cardiotocographic monitoring of labour. Bedside equipment will be reduced and widely shifted to a centralised control room by introduction of the system. All installations are of high flexibility to leave room for future developments in medical engineering and for necessary services. -- Large-scale use of the new technique with capacity utilisation of the hardware involved will be ensured by appropriate upgrading of labour room personnel as well as by techno-organisational improvement of work and minimisation of expenses on diagnosis and monitoring. The measures proposed work according to expectation and lead to good perinatological results.", "contents": "[Cardiotocography -- proposal of system solution (author's transl)]. A system solution with new technical details and new demands on space distribution is proposed for the use of antenatal cardiotocography and cardiotocographic monitoring of labour. Bedside equipment will be reduced and widely shifted to a centralised control room by introduction of the system. All installations are of high flexibility to leave room for future developments in medical engineering and for necessary services. -- Large-scale use of the new technique with capacity utilisation of the hardware involved will be ensured by appropriate upgrading of labour room personnel as well as by techno-organisational improvement of work and minimisation of expenses on diagnosis and monitoring. The measures proposed work according to expectation and lead to good perinatological results."}
{"id": "PMID:484096", "title": "[Comparison between immunological pregnancy test and ultrasonic examination for determination of early pregnancy (author's transl)].", "content": "Ultrasonic B-scan examination (Vidoson) and the Gravimun semiquantitative HCG test were applied simultaneously to 107 women, between the 35th and 95th day form menstruation. Control checks were made after another ten and 20 days. The findings so far recorded are likely to support the conclusion that the sensitivity of ultrasonography is higher than that of semiquantitative detection of HCG from urine and, therefore, more applicable to diagnosis of early pregnancy or assessment of disorders in early pregnancy.", "contents": "[Comparison between immunological pregnancy test and ultrasonic examination for determination of early pregnancy (author's transl)]. Ultrasonic B-scan examination (Vidoson) and the Gravimun semiquantitative HCG test were applied simultaneously to 107 women, between the 35th and 95th day form menstruation. Control checks were made after another ten and 20 days. The findings so far recorded are likely to support the conclusion that the sensitivity of ultrasonography is higher than that of semiquantitative detection of HCG from urine and, therefore, more applicable to diagnosis of early pregnancy or assessment of disorders in early pregnancy."}
{"id": "PMID:484097", "title": "[Incidence of gestational diabetes and changes in insulin secretion of pregnant women (author's transl)].", "content": "The 50-g oGTT was applied to pregnant women suspected of having contracted diabetes. Carbohydrate tolerances were pathological in 23.6 per cent of all probands and in borderline vicinity in 17.9 per cent. The oral glucose tainting test thus worked according to expectation by revealing a relatiively high frequency of disorders of the carbohydrate tolerance. Yet, impaired insulin secretion was established rarely, with high response having been recorded from only seven per cent and delayed insulin secretion from 4.1 per cent. Low response was not found at all. No correlations were found to exist, by the 50-g oGTT, between disorders of the carbohydrate tolerance and insulin secretion. The groups involved differed but little with regard to their IRI mean value curves. Sugar excretion in urine was found to be increased with significance in response to pathological carbohydrate tolerance in early pregnancy and may be used as a complementary criterion for diagnosis.", "contents": "[Incidence of gestational diabetes and changes in insulin secretion of pregnant women (author's transl)]. The 50-g oGTT was applied to pregnant women suspected of having contracted diabetes. Carbohydrate tolerances were pathological in 23.6 per cent of all probands and in borderline vicinity in 17.9 per cent. The oral glucose tainting test thus worked according to expectation by revealing a relatiively high frequency of disorders of the carbohydrate tolerance. Yet, impaired insulin secretion was established rarely, with high response having been recorded from only seven per cent and delayed insulin secretion from 4.1 per cent. Low response was not found at all. No correlations were found to exist, by the 50-g oGTT, between disorders of the carbohydrate tolerance and insulin secretion. The groups involved differed but little with regard to their IRI mean value curves. Sugar excretion in urine was found to be increased with significance in response to pathological carbohydrate tolerance in early pregnancy and may be used as a complementary criterion for diagnosis."}
{"id": "PMID:484098", "title": "[Influence of na-salicylate on uterine motility in labour (author's transl)].", "content": "A dosage of 10 mg/kg body weight Na-salicylate has no depressive effect on uterine motility. The examination carried out intra partum in the condition of normal uterine activity support our opinion that relatively high doses of 3--5 g per day or the simultaneous administration of beta-adrenergical substances are necessary to obtain an effective tocolysis.", "contents": "[Influence of na-salicylate on uterine motility in labour (author's transl)]. A dosage of 10 mg/kg body weight Na-salicylate has no depressive effect on uterine motility. The examination carried out intra partum in the condition of normal uterine activity support our opinion that relatively high doses of 3--5 g per day or the simultaneous administration of beta-adrenergical substances are necessary to obtain an effective tocolysis."}
{"id": "PMID:484099", "title": "[Planimetric analysis of labour induced by means of prostaglandin F 2 alpha and oxytocin (author's transl)].", "content": "Planimetry has been used to analyse the tocographic tracings recorded from 29 deliveries induced by means of prostaglandin F 2 alpha as well as those recorded from 28 deliveries induced by means of oxytocin. The contraction-causing effect of prostaglandin was found to be milder than that of oxytocin. Prostaglandin F 2 alpha, consequently, proved to be superior to oxytocin in handling placental insufficiency.", "contents": "[Planimetric analysis of labour induced by means of prostaglandin F 2 alpha and oxytocin (author's transl)]. Planimetry has been used to analyse the tocographic tracings recorded from 29 deliveries induced by means of prostaglandin F 2 alpha as well as those recorded from 28 deliveries induced by means of oxytocin. The contraction-causing effect of prostaglandin was found to be milder than that of oxytocin. Prostaglandin F 2 alpha, consequently, proved to be superior to oxytocin in handling placental insufficiency."}
{"id": "PMID:484100", "title": "[Localisation of placenta by liquid crystal thermography (author's transl)].", "content": "The efficiency of liquid crystals in placenta localisation was checked by comparison of thermographic results with those obtained from using the ultrasonic B-scan method. Ther  results obtained from the two techniques were in fair agreement in 93 of 106 cases or 87.7 per cent. The highest amount of agreement was recorded from the group with anterior-wall placenta. For placenta localisation the sensitivity of liquid crystal thermography was found to be inferior to that of the ultrasonic B-scan method.", "contents": "[Localisation of placenta by liquid crystal thermography (author's transl)]. The efficiency of liquid crystals in placenta localisation was checked by comparison of thermographic results with those obtained from using the ultrasonic B-scan method. Ther  results obtained from the two techniques were in fair agreement in 93 of 106 cases or 87.7 per cent. The highest amount of agreement was recorded from the group with anterior-wall placenta. For placenta localisation the sensitivity of liquid crystal thermography was found to be inferior to that of the ultrasonic B-scan method."}
{"id": "PMID:484103", "title": "Antibiotic resistance of salmonellae in Czechoslovakia -- situation and prospects.", "content": "Antibiotic resistance of non-typhoid Salmonellae is in Czechoslovakia continuously monitored by a computer and its transferability is studied, on an all-nation level, within the Federal Plan of Scientific Investigation and Development. Restance and its transferability was surveyed in strains of Salmonella from humans, from farm animals, from the food chain as well as from surface and waste waters. Proportion of antibiotic-resistant Salmonella strains did not, in general, increase during the four-year period of 1973 to 1977. While resistant strains from humans as well as farm animals show a high degree of transferability, strains from food samples lack, in general, the transfer ability. This might be inferred that strains causing human salmonellosis which are carried to man trough foodchain, gain their character of transferability directly in the gut, i.e. from E. coli strains, and mainly due to presence of therapeutically given antibiotics.", "contents": "Antibiotic resistance of salmonellae in Czechoslovakia -- situation and prospects. Antibiotic resistance of non-typhoid Salmonellae is in Czechoslovakia continuously monitored by a computer and its transferability is studied, on an all-nation level, within the Federal Plan of Scientific Investigation and Development. Restance and its transferability was surveyed in strains of Salmonella from humans, from farm animals, from the food chain as well as from surface and waste waters. Proportion of antibiotic-resistant Salmonella strains did not, in general, increase during the four-year period of 1973 to 1977. While resistant strains from humans as well as farm animals show a high degree of transferability, strains from food samples lack, in general, the transfer ability. This might be inferred that strains causing human salmonellosis which are carried to man trough foodchain, gain their character of transferability directly in the gut, i.e. from E. coli strains, and mainly due to presence of therapeutically given antibiotics."}
{"id": "PMID:484105", "title": "[On the phenotypical characteristics of human pasteurella, and pasteurella-like isolates (author's transl)].", "content": "Fourty-two human isolates that had been designated Pasteurella, or pasteurella-like organisms in the bacteriological routine laboratory were phenotypically characterized considering conventional, morphological and physiological features, and respiratory quinones. Thirty-seven of these strains fitted into the large traditional species, P. multocida, the majority of them being associated with alterations of the respiratory tract, and the rest with intestinal diseases, or putrid wound secretions mostly following animal bite or scratch lesions. Two strains isolated from sputum, or sinus maxillaris punctate, respectively, were P. ureae, and one strain recovered from a septicemic blood samle proved to be Cardiobacterium hominis. A pasteurella-like strain isolated from putrid sputum and an unusual organism that had been isolated from the cerebrospinal fluid of an infant with putrid meningitis remained unidentified. The bacteriological data are discussed with respect to the diagnostics of Pasteurella and similar organisms and especially, the range of phenotypical variation within the species, P. multocida.", "contents": "[On the phenotypical characteristics of human pasteurella, and pasteurella-like isolates (author's transl)]. Fourty-two human isolates that had been designated Pasteurella, or pasteurella-like organisms in the bacteriological routine laboratory were phenotypically characterized considering conventional, morphological and physiological features, and respiratory quinones. Thirty-seven of these strains fitted into the large traditional species, P. multocida, the majority of them being associated with alterations of the respiratory tract, and the rest with intestinal diseases, or putrid wound secretions mostly following animal bite or scratch lesions. Two strains isolated from sputum, or sinus maxillaris punctate, respectively, were P. ureae, and one strain recovered from a septicemic blood samle proved to be Cardiobacterium hominis. A pasteurella-like strain isolated from putrid sputum and an unusual organism that had been isolated from the cerebrospinal fluid of an infant with putrid meningitis remained unidentified. The bacteriological data are discussed with respect to the diagnostics of Pasteurella and similar organisms and especially, the range of phenotypical variation within the species, P. multocida."}
{"id": "PMID:484106", "title": "[Enzymological examinations on Clostrium oncolyticum M 55 ATCC 13,732 (author's transl)].", "content": "Since excenzymes are mainly involved in oncolysis by Cl. oncolyticum M 55 ATCC 13,732 the enzyme production of the strain should be examined under different growth conditions. The excretion of a protease and of two nucleases was observed in the nutrient medium for 72 h and the results obtained in a nitrogen atmosphere were compared with those obtained in an oxygen atmosphere. The cleavage of protein and of peptides was investigated by means of TLC. The importance of the exoenzymes for the oncolytic effect of Cl. oncolyticum M 55 was confirmed by animal experiments.", "contents": "[Enzymological examinations on Clostrium oncolyticum M 55 ATCC 13,732 (author's transl)]. Since excenzymes are mainly involved in oncolysis by Cl. oncolyticum M 55 ATCC 13,732 the enzyme production of the strain should be examined under different growth conditions. The excretion of a protease and of two nucleases was observed in the nutrient medium for 72 h and the results obtained in a nitrogen atmosphere were compared with those obtained in an oxygen atmosphere. The cleavage of protein and of peptides was investigated by means of TLC. The importance of the exoenzymes for the oncolytic effect of Cl. oncolyticum M 55 was confirmed by animal experiments."}
{"id": "PMID:484108", "title": "[Microbiological and parasitic risks associated with the stay in hot countries (author's transl)].", "content": "The microbiological and parasitic risks associated with the tremendous increase in the tourist traffic from West Germany and Austria into warmer countries are considerable even in the case of shorter stays, as has been confirmed by observations made on those returning from such areas. The most important groups of diseases -- worm diseases, amoebiasis, and malaria -- have maintained this ranking in Central and South America; in Africa, especially in the east, the incidence of malaria tropica is increasing. The proportions for Austria show that even numerically there are no great differences compared with West Germany. It seems that, as far as malaria is concerned, efficient prophylactic measures and reliable information are absent; at any rate, the cases of malaria tropica were severe and in the main no prophylactic measures had been taken. With the exception of Japan, the risk of hepatitis in all warmer countries and in the East in general is many times higher than in the Federal Republic of Germany and the length of stay is partly a determining factor. For instance, the risk of hepatitis is roughly forty times higher in India. All figures shown in this survey are supported by tables, literature, and personal experience.", "contents": "[Microbiological and parasitic risks associated with the stay in hot countries (author's transl)]. The microbiological and parasitic risks associated with the tremendous increase in the tourist traffic from West Germany and Austria into warmer countries are considerable even in the case of shorter stays, as has been confirmed by observations made on those returning from such areas. The most important groups of diseases -- worm diseases, amoebiasis, and malaria -- have maintained this ranking in Central and South America; in Africa, especially in the east, the incidence of malaria tropica is increasing. The proportions for Austria show that even numerically there are no great differences compared with West Germany. It seems that, as far as malaria is concerned, efficient prophylactic measures and reliable information are absent; at any rate, the cases of malaria tropica were severe and in the main no prophylactic measures had been taken. With the exception of Japan, the risk of hepatitis in all warmer countries and in the East in general is many times higher than in the Federal Republic of Germany and the length of stay is partly a determining factor. For instance, the risk of hepatitis is roughly forty times higher in India. All figures shown in this survey are supported by tables, literature, and personal experience."}
{"id": "PMID:484110", "title": "[Germinal change and its significance in the hospital (author's transl)].", "content": "To receive objective informations about clinically supposed difference of bacteria spectrum in hospital infections within the last 20 years, we made statistical comparatively investigations between varying periods and in different fields. In the whole material of the Medical Department we found an increase of Proteus, Pseudomonas and Klebsiella (p = 0,01%) and an increase of Candida while Staphylococci, Streptococci, Pneumococci, Clostridium and Meningococci have clearly decreased. In the Department of Nephrology we found a significant increase of Proteus and Pseudomonas (in regard to the manifold findings also of Klebsiella) and a significant decrease of Enterococci (in regard to the manifold findings also of E. coli). In the Reanimation Department there were in opposite to a strong increase of Candida, followed by Pseudomonas, Klebsiella, Enterobacter and Pneumococci, a decrease of Staphylococcus aureus, Enterococci and Streptococci in the sputum findings. In the Urological Clinic while the period of report Pseudomonas and Enterobacter increased significantly, Staphlococcus aureus and Streptococci decreased significantly. In the Neurosurgical Clinic the part of the gramnegative germs of all germinal isolations increased from 8,3% to 51,5% at which especially E. coli, Enterobacter and Pseudomonas were more often found.", "contents": "[Germinal change and its significance in the hospital (author's transl)]. To receive objective informations about clinically supposed difference of bacteria spectrum in hospital infections within the last 20 years, we made statistical comparatively investigations between varying periods and in different fields. In the whole material of the Medical Department we found an increase of Proteus, Pseudomonas and Klebsiella (p = 0,01%) and an increase of Candida while Staphylococci, Streptococci, Pneumococci, Clostridium and Meningococci have clearly decreased. In the Department of Nephrology we found a significant increase of Proteus and Pseudomonas (in regard to the manifold findings also of Klebsiella) and a significant decrease of Enterococci (in regard to the manifold findings also of E. coli). In the Reanimation Department there were in opposite to a strong increase of Candida, followed by Pseudomonas, Klebsiella, Enterobacter and Pneumococci, a decrease of Staphylococcus aureus, Enterococci and Streptococci in the sputum findings. In the Urological Clinic while the period of report Pseudomonas and Enterobacter increased significantly, Staphlococcus aureus and Streptococci decreased significantly. In the Neurosurgical Clinic the part of the gramnegative germs of all germinal isolations increased from 8,3% to 51,5% at which especially E. coli, Enterobacter and Pseudomonas were more often found."}
{"id": "PMID:484112", "title": "[Possibilities and limitations in the localisation of sources of infections (author's transl)].", "content": "In latent microbial hospitalism environmental checks of the patients, the staff and objects permit detection of pathogenic or potentially pathogenic bacteria which are silent epidemiologically. \"One's own body\" as a source of infection has not yet been sufficiently investigated in connection with auto-infections. Taking two staphylococcal epidemics as examples, reference is made to the monogenic and heterogenic forms of hospitalism as well as to the necessity of accurately identifying the strains for the purpose of localising the source of infection. In the monogenic form only one type of bacterial species is present (Table 2). In heterogenic hospitalism, infections are caused by various different species of bacteria or also by a single species. If this is the case, various biotypes, serotypes and phagotypes exist (Table 1). In addition, simple possibilities of biotyping the staphylococci are demonstrated. Defensive measures can be adopted selectively only when the epidemiology and the source of infection are known.", "contents": "[Possibilities and limitations in the localisation of sources of infections (author's transl)]. In latent microbial hospitalism environmental checks of the patients, the staff and objects permit detection of pathogenic or potentially pathogenic bacteria which are silent epidemiologically. \"One's own body\" as a source of infection has not yet been sufficiently investigated in connection with auto-infections. Taking two staphylococcal epidemics as examples, reference is made to the monogenic and heterogenic forms of hospitalism as well as to the necessity of accurately identifying the strains for the purpose of localising the source of infection. In the monogenic form only one type of bacterial species is present (Table 2). In heterogenic hospitalism, infections are caused by various different species of bacteria or also by a single species. If this is the case, various biotypes, serotypes and phagotypes exist (Table 1). In addition, simple possibilities of biotyping the staphylococci are demonstrated. Defensive measures can be adopted selectively only when the epidemiology and the source of infection are known."}
{"id": "PMID:484113", "title": "[Physicochemical fundamentals on the cleaning of hard surfaces (author's transl)].", "content": "The primary process in the cleaning of hard surfaces is the adsorption of the active substance of the detergent at the interfaces: liquid/gas, liquid/liquid, liquid/solid. This primary step in the cleaning process induces secondary processes which in turn are responsible for the soil removal from the surface of the substrate. The first requirement for a cleaning effect is that the dirt and the substrate be well wetted by the cleaning solution as a result of a reduction in surface tension and of the adsorption at the solid/liquid interface. The further secondary processes which effect the removal of dirt vary considerably, depending on the type of dirt (e.g. oils, pigments). In the case of oily and greasy dirt, rolling-up penetration, formation of mixed phases, emulsification and solubilization are of importance. For pigments, the surface pressure of the adsorption layer and the electrostatic repulsion are the determining factors. The processes of pigment dispersion, emulsification and solubilization are, in addition, extremely important for the stabilization of the dirty wash bath and the prevention of deposits on the substrate. The essential active substances of the detergents are surfactant and complexing agents, the first being unspecifically adsorbed by hydrophobic interactions, and the latter specifically by polar interactions. They influence one another mutually and are responsible for an optimal cleaning effect when they occur in suitable mixing ratios; the special constitutional characteristics of the individual surfactant and complexing agents, must also be considered.", "contents": "[Physicochemical fundamentals on the cleaning of hard surfaces (author's transl)]. The primary process in the cleaning of hard surfaces is the adsorption of the active substance of the detergent at the interfaces: liquid/gas, liquid/liquid, liquid/solid. This primary step in the cleaning process induces secondary processes which in turn are responsible for the soil removal from the surface of the substrate. The first requirement for a cleaning effect is that the dirt and the substrate be well wetted by the cleaning solution as a result of a reduction in surface tension and of the adsorption at the solid/liquid interface. The further secondary processes which effect the removal of dirt vary considerably, depending on the type of dirt (e.g. oils, pigments). In the case of oily and greasy dirt, rolling-up penetration, formation of mixed phases, emulsification and solubilization are of importance. For pigments, the surface pressure of the adsorption layer and the electrostatic repulsion are the determining factors. The processes of pigment dispersion, emulsification and solubilization are, in addition, extremely important for the stabilization of the dirty wash bath and the prevention of deposits on the substrate. The essential active substances of the detergents are surfactant and complexing agents, the first being unspecifically adsorbed by hydrophobic interactions, and the latter specifically by polar interactions. They influence one another mutually and are responsible for an optimal cleaning effect when they occur in suitable mixing ratios; the special constitutional characteristics of the individual surfactant and complexing agents, must also be considered."}
{"id": "PMID:484114", "title": "[Mechanism of antimicrobial active components (author's transl)].", "content": "The extreme chemical heterogeneity of antimicrobial inhibitors may be compared with the large heterogeneity of the sites of attack. From the host of questions regarding the mechanism of action three problems are selected at random for discussion: 1. Penetration or transport of inhibitors into the cell. At least some of the inhibitors must penetrate into the cell and find their way to very specific points of action; and it does not go without saying that the antibiotics reach their targets without previously reacting with other cell components or without being \"trapped\" by the cytoplasmic membrane. In some instances an active transport, i.e. a \"misuse\" by antibiotics of a transport system for a substrate can be demonstrated. 2. Distribution of the targets in the cell. Distribution of the inhibitors over the possible targets is not uniform. The question why this is so remains unanswered. 3. The membrane as the point of attack for inhibitors. The probability that an inhibitor has a bactericidal effect also on resting cells is greatest with substances that act on the membrane. The question as to the function of the antibiotics for the active cell arises and the \"defence function\" of the antibiotics is called in question.", "contents": "[Mechanism of antimicrobial active components (author's transl)]. The extreme chemical heterogeneity of antimicrobial inhibitors may be compared with the large heterogeneity of the sites of attack. From the host of questions regarding the mechanism of action three problems are selected at random for discussion: 1. Penetration or transport of inhibitors into the cell. At least some of the inhibitors must penetrate into the cell and find their way to very specific points of action; and it does not go without saying that the antibiotics reach their targets without previously reacting with other cell components or without being \"trapped\" by the cytoplasmic membrane. In some instances an active transport, i.e. a \"misuse\" by antibiotics of a transport system for a substrate can be demonstrated. 2. Distribution of the targets in the cell. Distribution of the inhibitors over the possible targets is not uniform. The question why this is so remains unanswered. 3. The membrane as the point of attack for inhibitors. The probability that an inhibitor has a bactericidal effect also on resting cells is greatest with substances that act on the membrane. The question as to the function of the antibiotics for the active cell arises and the \"defence function\" of the antibiotics is called in question."}
{"id": "PMID:484115", "title": "[Bovine mastitis: a problem of veterinary medicine, economics and food hygiene (author's transl)].", "content": "Veterinary medicine, dairying, and consumers are likewise interested in mastitis control. The veterinary point of view includes health and function of the udder as well as the production of milk of hygienic quality. Investigations in 6 dairy farms showed that a mastitis control program improved the udder health and resulted in economic benefits. Up to 67% of the subclinical infected udder quarters could be eliminated by prophylactical control measures, especially teat dipping and dry cow therapy. The frequency of clinical udder infections was reduced by about 50%. Furthermore in the first year 5.1% and 7.1% increase of milk and fat yield respectively could be noticed for cows being subject to the program for the whole time. These values could not be reached in the second year. In one herd the milk production increased by about 10% after a control period of two years. The owner of this herd (ca. 35 cows), who was very engaged in the program, obtained an economical benefit of ca. 3500 DM in the first and 7300 DM in the second year of the program. This realised a profit of1.85 DM in the first year and 3.85 DM in the second year per each DM cost in the control program. Mastitis as well as control measures do not implicate a special risk for the human health, because food and drug regulations enable the protection of the consumer in an optimal manner.", "contents": "[Bovine mastitis: a problem of veterinary medicine, economics and food hygiene (author's transl)]. Veterinary medicine, dairying, and consumers are likewise interested in mastitis control. The veterinary point of view includes health and function of the udder as well as the production of milk of hygienic quality. Investigations in 6 dairy farms showed that a mastitis control program improved the udder health and resulted in economic benefits. Up to 67% of the subclinical infected udder quarters could be eliminated by prophylactical control measures, especially teat dipping and dry cow therapy. The frequency of clinical udder infections was reduced by about 50%. Furthermore in the first year 5.1% and 7.1% increase of milk and fat yield respectively could be noticed for cows being subject to the program for the whole time. These values could not be reached in the second year. In one herd the milk production increased by about 10% after a control period of two years. The owner of this herd (ca. 35 cows), who was very engaged in the program, obtained an economical benefit of ca. 3500 DM in the first and 7300 DM in the second year of the program. This realised a profit of1.85 DM in the first year and 3.85 DM in the second year per each DM cost in the control program. Mastitis as well as control measures do not implicate a special risk for the human health, because food and drug regulations enable the protection of the consumer in an optimal manner."}
{"id": "PMID:484116", "title": "[Autoradiographic investigations on the effect of city smog extract on DNA synthesis and cell cycle of mammalian cells in vitro. I. Effect of city smog extract on DNA synthesis of kidney- and embryonic cells of the golden hamster in vitro (author's transl)].", "content": "We analysed the effect of city smog extract from D\u00fcsseldorf on DNA synthesis of mammalian cells in vitro. Airborne dust was extracted with aceton and thereafter transferred to dimethylsulfoxide. Dosage was calculated according to the benzo(a)pyrene content. We used logarithmically growing cultures of hamster kidney and embryonic cells. DNA synthesis was determined autoradiographically by incorporation of 3H-Thymidine. We found that city smog extract exerted a dose-dependent cytotoxic effect leading to a decrease of DNA synthesizing cells. High concentrations of city smog extract induced cell necrosis and suppressed DNA synthesis completely. Moderate doses of extract caused a dose-dependent, but temporary cessation of DNA synthesis. Cells resumed DNA synthesis after a certain delay. Low concentrations of city smog extract induced no detectable effects. Inhibition of DNA synthesis was evident already one hour after addition of extract. Therefore a direct effect on DNA metabolism could be supposed. Furthermore, exposed cultures demonstrated a delay in entrance of new cells into the DNA synthesis. Alterations in DNA synthesis could be of great importance for carcinogenesis, especially if we take in consideration the content of carcinogens in city smog extract.", "contents": "[Autoradiographic investigations on the effect of city smog extract on DNA synthesis and cell cycle of mammalian cells in vitro. I. Effect of city smog extract on DNA synthesis of kidney- and embryonic cells of the golden hamster in vitro (author's transl)]. We analysed the effect of city smog extract from D\u00fcsseldorf on DNA synthesis of mammalian cells in vitro. Airborne dust was extracted with aceton and thereafter transferred to dimethylsulfoxide. Dosage was calculated according to the benzo(a)pyrene content. We used logarithmically growing cultures of hamster kidney and embryonic cells. DNA synthesis was determined autoradiographically by incorporation of 3H-Thymidine. We found that city smog extract exerted a dose-dependent cytotoxic effect leading to a decrease of DNA synthesizing cells. High concentrations of city smog extract induced cell necrosis and suppressed DNA synthesis completely. Moderate doses of extract caused a dose-dependent, but temporary cessation of DNA synthesis. Cells resumed DNA synthesis after a certain delay. Low concentrations of city smog extract induced no detectable effects. Inhibition of DNA synthesis was evident already one hour after addition of extract. Therefore a direct effect on DNA metabolism could be supposed. Furthermore, exposed cultures demonstrated a delay in entrance of new cells into the DNA synthesis. Alterations in DNA synthesis could be of great importance for carcinogenesis, especially if we take in consideration the content of carcinogens in city smog extract."}
{"id": "PMID:484117", "title": "[Field tests carried out to determine the occupational exposure to vanadium (author's transl)].", "content": "In a metallurgic plant we analysed blood samples, urine and finger-nails of 54 workers exposed to vanadium. On the basis of orientating dust measurements it can be assumed that the results obtained were, as a rule, clearly lower than the MAC values for vanadium pentoxide dust. The median vanadium concentration in whole-blood was 2.9 micrograms/l. This indicates that the exposed persons differ significantly from the control group. The median vanadium concentration measured in urine was 37.8 micrograms/l. This means that there is a significant difference in comparison with the control group (0.8 micrograms/l). In order to obtain values of greater reliability, the vanadium concentration resulting from the analysis of spontaneous urine samples was to be referred to the creatinine content. Conversion yields a medium vanadium concentration of 33.9 and 0.6 micrograms/g creatinine for the exposed workers and normal persons respectively. The cystine content found in the finger-nails of persons occupationally exposed to vanadium was significantly reduced with respect to the comparative group (8.9 against 9.9 mg cystine per 100 mg finger-nails). Within the groups of persons examined no correlation is found to exist between the cystine content of the finger-nails and the age of the persons, nor between the vanadium concentrations in blood and urine. After a weekend without exposure the vanadium concentrations in blood and urine dropped. In general, this drop was the more pronounced the higher the initial value was. Roughly two to four days after the occupational exposure had ceased, the values dropped to the half of the initial value. Based on the test results available, the vanadium concentrations in blood and urine reflect the extent of an occupational vanadium exposure and are suitable indicators for estimating the potential threat caused by this heavy metal.", "contents": "[Field tests carried out to determine the occupational exposure to vanadium (author's transl)]. In a metallurgic plant we analysed blood samples, urine and finger-nails of 54 workers exposed to vanadium. On the basis of orientating dust measurements it can be assumed that the results obtained were, as a rule, clearly lower than the MAC values for vanadium pentoxide dust. The median vanadium concentration in whole-blood was 2.9 micrograms/l. This indicates that the exposed persons differ significantly from the control group. The median vanadium concentration measured in urine was 37.8 micrograms/l. This means that there is a significant difference in comparison with the control group (0.8 micrograms/l). In order to obtain values of greater reliability, the vanadium concentration resulting from the analysis of spontaneous urine samples was to be referred to the creatinine content. Conversion yields a medium vanadium concentration of 33.9 and 0.6 micrograms/g creatinine for the exposed workers and normal persons respectively. The cystine content found in the finger-nails of persons occupationally exposed to vanadium was significantly reduced with respect to the comparative group (8.9 against 9.9 mg cystine per 100 mg finger-nails). Within the groups of persons examined no correlation is found to exist between the cystine content of the finger-nails and the age of the persons, nor between the vanadium concentrations in blood and urine. After a weekend without exposure the vanadium concentrations in blood and urine dropped. In general, this drop was the more pronounced the higher the initial value was. Roughly two to four days after the occupational exposure had ceased, the values dropped to the half of the initial value. Based on the test results available, the vanadium concentrations in blood and urine reflect the extent of an occupational vanadium exposure and are suitable indicators for estimating the potential threat caused by this heavy metal."}
{"id": "PMID:484118", "title": "[Rhizospheric organism under the influence of environmental pollution. II. Physiologic efficiency of the microbial biocenosis in fungicide containing soil (author's transl)].", "content": "Measuring the potential soil respiration according to the incubator method allows the physiological performance of the celluloytic microflora to be determined. Laboratory tests were carried out to analyse catabolic processes after soil samples had been enriched with native cellulose + fungides. As the temporary capacity of mineralization primarily induced by the substrate invariably gives rise to a substrate-specific \"change in position\" in the microbial biocenosis, the measured values cannot furnish a parameter for microbiological comparisons of the soil content. Preliminary analyses provided information on the processes of depolymerisation of various C-sources and characterized the celluloysis as well as the order of respiration of its monomer. The rising respiration curve markedly drops off after 12 days, because with the CO2 development at its maximum, total and partial respiration alternate, a phenomenon that was verified in soils incubated with glucose 14C (Keefer and Mortensen, 1963; Macura, 1963; Simonart and Mayaudon, 1958). The test results obtained with fungicide-treated soils (dithiocarbamine acid derivatives) allow the conclusion that the fungicides exercise a varying influence: in the cellulolysis the activity of the cellulase systems is reduced depending on the active substance and concentration, but what is surprising is that respiration is stimulated; this applies to amounts of pesticides being 0.75 and 1.5 times the practical quantity applied. Nor have the \"dissociation tests\" with cellobiose, which is supposed to be the final link of celluloysis, shown any inhibition of the 1.4--glucodimerase or high respiration values comparable with the glucose metabolism. Tests with cellulases (Cx with dimerase function, Messrs. Roehm and Haas, Darmstadt), which are at present being carried out, are expected to support these observations experimentally. Investigations intended to clarify whether an additional administration of Mn2+ and Fe3+ in the presence of Maneb and Ferbam permits interference with possible adsorption and exchange processes have not yet yielded any generally valid results. The results of the open-field investigations also tend to indicate that the mentioned dithio-carbamates determine the magnitude and the extent of the physiological and CO2-ecological processes to a varying degree; it can be seen that a remarkable \"capacity fo compensation\" is discernible in the succession of the investigated phases of soil-physiological processes which we tried to clarify.", "contents": "[Rhizospheric organism under the influence of environmental pollution. II. Physiologic efficiency of the microbial biocenosis in fungicide containing soil (author's transl)]. Measuring the potential soil respiration according to the incubator method allows the physiological performance of the celluloytic microflora to be determined. Laboratory tests were carried out to analyse catabolic processes after soil samples had been enriched with native cellulose + fungides. As the temporary capacity of mineralization primarily induced by the substrate invariably gives rise to a substrate-specific \"change in position\" in the microbial biocenosis, the measured values cannot furnish a parameter for microbiological comparisons of the soil content. Preliminary analyses provided information on the processes of depolymerisation of various C-sources and characterized the celluloysis as well as the order of respiration of its monomer. The rising respiration curve markedly drops off after 12 days, because with the CO2 development at its maximum, total and partial respiration alternate, a phenomenon that was verified in soils incubated with glucose 14C (Keefer and Mortensen, 1963; Macura, 1963; Simonart and Mayaudon, 1958). The test results obtained with fungicide-treated soils (dithiocarbamine acid derivatives) allow the conclusion that the fungicides exercise a varying influence: in the cellulolysis the activity of the cellulase systems is reduced depending on the active substance and concentration, but what is surprising is that respiration is stimulated; this applies to amounts of pesticides being 0.75 and 1.5 times the practical quantity applied. Nor have the \"dissociation tests\" with cellobiose, which is supposed to be the final link of celluloysis, shown any inhibition of the 1.4--glucodimerase or high respiration values comparable with the glucose metabolism. Tests with cellulases (Cx with dimerase function, Messrs. Roehm and Haas, Darmstadt), which are at present being carried out, are expected to support these observations experimentally. Investigations intended to clarify whether an additional administration of Mn2+ and Fe3+ in the presence of Maneb and Ferbam permits interference with possible adsorption and exchange processes have not yet yielded any generally valid results. The results of the open-field investigations also tend to indicate that the mentioned dithio-carbamates determine the magnitude and the extent of the physiological and CO2-ecological processes to a varying degree; it can be seen that a remarkable \"capacity fo compensation\" is discernible in the succession of the investigated phases of soil-physiological processes which we tried to clarify."}
{"id": "PMID:484119", "title": "[Studies to investigate the ecological importance of the mass development of Hydrodictyon reticulatum in infiltration basins for drinking water. I. Metabolic products and their effect on various types of bacteria (author's transl)].", "content": "Cells of the green freshwater alga H. reticulatum, harvested from their natural habitat, were found to have antibacterial activity. The active substances seem to be lipophilic, because ethanol extracts were more effective, especially against Bac. subtilis, than water extracts (Table 4). The different effects of water extracts, which were tested after various dilution steps (Table 1--3) presume, that there must be a second active substance. We suppose, that it is correlated with chlorophyll derivatives. Culture filtrate from a strain, grown under laboratory conditions, was tested in the same way. Concentrated tenfold it was active against several grampositive strains of bacteria.", "contents": "[Studies to investigate the ecological importance of the mass development of Hydrodictyon reticulatum in infiltration basins for drinking water. I. Metabolic products and their effect on various types of bacteria (author's transl)]. Cells of the green freshwater alga H. reticulatum, harvested from their natural habitat, were found to have antibacterial activity. The active substances seem to be lipophilic, because ethanol extracts were more effective, especially against Bac. subtilis, than water extracts (Table 4). The different effects of water extracts, which were tested after various dilution steps (Table 1--3) presume, that there must be a second active substance. We suppose, that it is correlated with chlorophyll derivatives. Culture filtrate from a strain, grown under laboratory conditions, was tested in the same way. Concentrated tenfold it was active against several grampositive strains of bacteria."}
{"id": "PMID:484120", "title": "[Microbial settlement of paint- and building-materials in the sphere of drinking water. 3. Macrocolonies on the joint in a drinking-water-reservoir covered with tile (author's transl)].", "content": "The microbial growth in form of macrocolonies on the joint of a drinking-water-reservoir covered with tile could be observed repeatedly. The slime was examined microbiologically. The origin of the carbon sources was determined by radiocarbon test.", "contents": "[Microbial settlement of paint- and building-materials in the sphere of drinking water. 3. Macrocolonies on the joint in a drinking-water-reservoir covered with tile (author's transl)]. The microbial growth in form of macrocolonies on the joint of a drinking-water-reservoir covered with tile could be observed repeatedly. The slime was examined microbiologically. The origin of the carbon sources was determined by radiocarbon test."}
{"id": "PMID:484122", "title": "[Metastases in the hypophysis].", "content": "Among 1521 malignant extracranial tumours, 64 metastases (4.2%) were found in the pituitary. The most frequent occurrence was found for carcinomas of the breast (21.4%) and for bronchial malignomas (5.9%). A statistically significant difference with regard to the location in the anterior and the posterior lobe was not found in our own patients. A remarkable fact is the high degree of affection of the pituitary gland in case of malignant melanomas.", "contents": "[Metastases in the hypophysis]. Among 1521 malignant extracranial tumours, 64 metastases (4.2%) were found in the pituitary. The most frequent occurrence was found for carcinomas of the breast (21.4%) and for bronchial malignomas (5.9%). A statistically significant difference with regard to the location in the anterior and the posterior lobe was not found in our own patients. A remarkable fact is the high degree of affection of the pituitary gland in case of malignant melanomas."}
{"id": "PMID:484123", "title": "[Retinal blindness as a complication of carotid angiography].", "content": "Aortography and subsequent angiography of the A. carotis communis (Seldinger technique) was carried out in a 51-year-old man suffering from cerebrovascular insufficiency (transitory hemiparesis, disturbances in word finding). Complete interna occlusion from the carotid fork, no formation of collaterals in the ophthalmic artery region. After the last administration of the contrast medium loss of sight on the side of the carotid angiography (right side). Reappearing of retinal circulation only on the following day. Treatment with Radecol, Prednisolut, amyl nitrite. No return of slight, --Discussion of the two releasing possibilities; washing-in of embolic material and arterial spasms. A survey in the form of tables is given of nine similar complications which were found in the literature. Comprehensive bibliography.", "contents": "[Retinal blindness as a complication of carotid angiography]. Aortography and subsequent angiography of the A. carotis communis (Seldinger technique) was carried out in a 51-year-old man suffering from cerebrovascular insufficiency (transitory hemiparesis, disturbances in word finding). Complete interna occlusion from the carotid fork, no formation of collaterals in the ophthalmic artery region. After the last administration of the contrast medium loss of sight on the side of the carotid angiography (right side). Reappearing of retinal circulation only on the following day. Treatment with Radecol, Prednisolut, amyl nitrite. No return of slight, --Discussion of the two releasing possibilities; washing-in of embolic material and arterial spasms. A survey in the form of tables is given of nine similar complications which were found in the literature. Comprehensive bibliography."}
{"id": "PMID:484124", "title": "[Intervertebral disk displacement in children and young adults].", "content": "A report is given on 10 children and adolescents who were surgically treated because of prolapses of lumbar intervertebral discs. Besides a detailed anamnesis, the preoperative diagnosis was mainly based on general medical and neurological findings as well as X-ray pictures of the lumbar vertebral column. In case of the absence of radicular neurological failures and pecularities in the X-ray picture, e.g. signs of a spinal process of displacement, destructive changes in the osseous structures of the vertebral column, assimilation disturbances in the lumbosacral transition region, there is the indication for Dimer-X myelography. For all patients, histologically distinct to high-degree degenerative changes in the withdrawn vertebral disk material were found. Reliable signs of a traumatic genesis of the intervertebral disk prolapse were not seen.", "contents": "[Intervertebral disk displacement in children and young adults]. A report is given on 10 children and adolescents who were surgically treated because of prolapses of lumbar intervertebral discs. Besides a detailed anamnesis, the preoperative diagnosis was mainly based on general medical and neurological findings as well as X-ray pictures of the lumbar vertebral column. In case of the absence of radicular neurological failures and pecularities in the X-ray picture, e.g. signs of a spinal process of displacement, destructive changes in the osseous structures of the vertebral column, assimilation disturbances in the lumbosacral transition region, there is the indication for Dimer-X myelography. For all patients, histologically distinct to high-degree degenerative changes in the withdrawn vertebral disk material were found. Reliable signs of a traumatic genesis of the intervertebral disk prolapse were not seen."}
{"id": "PMID:484125", "title": "[Neurosurgical aspects of the apallic problem].", "content": "In the period from 1965 to 1974, 77 patients with an apallic syndrome were observed at the Surgical University Clinic of Innsbruck. Main cause was cerebral contusion (35 patients) followed by open fracture of the skull (7), acute subdural haematoma (7), aneurysm (7), epidural haematoma (6), cerebral tumours (4), perforating skull lesion and traumatic intracerebral haematoma (2 each). 43 patients died, 5 showed spontaneous healing and 9 a rehabilitation effect. Defect healings occurred 12 times. For 23 patients, the survival times were up to 3 months and for 4 patients one year. Patients with healed defects were followed up for more than 10 years. The calculation of the nursing costs as compared to a simple surgical case showed an eight times higher value. However, the financial expenditure for drugs and laboratory findings decreased considerably from about the forth week. The large amount of nursing means and the high cost for personnel remained. Of the 285 patients of the years 1965 to 1974, who were unconscious during a period of up to one week, 59 became apallic. Of these, 27 survival, three were healed, 8 in rehabilitation, 5 as permanent nursing cases and 2 as defect healing. No clinical or intrumental methods can be found by means of which a prediction is possible whether the patient will be healed or become will develop an apallic syndrome.", "contents": "[Neurosurgical aspects of the apallic problem]. In the period from 1965 to 1974, 77 patients with an apallic syndrome were observed at the Surgical University Clinic of Innsbruck. Main cause was cerebral contusion (35 patients) followed by open fracture of the skull (7), acute subdural haematoma (7), aneurysm (7), epidural haematoma (6), cerebral tumours (4), perforating skull lesion and traumatic intracerebral haematoma (2 each). 43 patients died, 5 showed spontaneous healing and 9 a rehabilitation effect. Defect healings occurred 12 times. For 23 patients, the survival times were up to 3 months and for 4 patients one year. Patients with healed defects were followed up for more than 10 years. The calculation of the nursing costs as compared to a simple surgical case showed an eight times higher value. However, the financial expenditure for drugs and laboratory findings decreased considerably from about the forth week. The large amount of nursing means and the high cost for personnel remained. Of the 285 patients of the years 1965 to 1974, who were unconscious during a period of up to one week, 59 became apallic. Of these, 27 survival, three were healed, 8 in rehabilitation, 5 as permanent nursing cases and 2 as defect healing. No clinical or intrumental methods can be found by means of which a prediction is possible whether the patient will be healed or become will develop an apallic syndrome."}
{"id": "PMID:484126", "title": "[Fusiform arterial dilations of the vessels of the posterior cranial fossa].", "content": "In a 28-year-old woman with symptomatic cerebral convulsions, vertebral angiography showed two spindle-like dilatations which are probably located in the vascular province of the A. cerebelli superior. The EEG showed convulsive activities, the PEG a beginning hydrocephalus. Neurological findings and fundoscopy were normal. Lues could be ruled out. Differentiation from a cerebral phlebectasia, which is also rare, was reliably possible. An association between the vascular dilatations and the convulsions is not assumed.", "contents": "[Fusiform arterial dilations of the vessels of the posterior cranial fossa]. In a 28-year-old woman with symptomatic cerebral convulsions, vertebral angiography showed two spindle-like dilatations which are probably located in the vascular province of the A. cerebelli superior. The EEG showed convulsive activities, the PEG a beginning hydrocephalus. Neurological findings and fundoscopy were normal. Lues could be ruled out. Differentiation from a cerebral phlebectasia, which is also rare, was reliably possible. An association between the vascular dilatations and the convulsions is not assumed."}
{"id": "PMID:484127", "title": "[Therapy problems in sphenoethmoidal meningoceles].", "content": "After a brief survey of the forms of cranial meningoencephaloceles, the current opinions on their development, symptoms and surgical therapy, a report is given on the case of a five-year-old boy suffering from a sphenoethmoidal meningocele of the size 3 x 3 x 4 cm. There was at the same time a wide median palate cleft, obviously a consequence of the meningoencephalocele. A further finding presented by the boy was a neuroradiologically diagnosed agenesis of the Corpus callosum and a median cheiloschisis. This suggests a central development disturbance as a primary cause. The attempt to perform an intracranial occlusion of the defect of the base, which was repeated twice, was not successful. It was only by a transpalatinal-transnasal extradural intervention (reposition of the meningocele after removing the epipharyngeal mucosa, blocking of the bony defect with a perforated tantalum plate) that the removal of the meningocele was successful. The closure of the palate cleft was then made by the oral surgeon. This surgical intervention may also be considered for the treatment of the transsphenoidal meningoencephalocele, which also presents many problems.", "contents": "[Therapy problems in sphenoethmoidal meningoceles]. After a brief survey of the forms of cranial meningoencephaloceles, the current opinions on their development, symptoms and surgical therapy, a report is given on the case of a five-year-old boy suffering from a sphenoethmoidal meningocele of the size 3 x 3 x 4 cm. There was at the same time a wide median palate cleft, obviously a consequence of the meningoencephalocele. A further finding presented by the boy was a neuroradiologically diagnosed agenesis of the Corpus callosum and a median cheiloschisis. This suggests a central development disturbance as a primary cause. The attempt to perform an intracranial occlusion of the defect of the base, which was repeated twice, was not successful. It was only by a transpalatinal-transnasal extradural intervention (reposition of the meningocele after removing the epipharyngeal mucosa, blocking of the bony defect with a perforated tantalum plate) that the removal of the meningocele was successful. The closure of the palate cleft was then made by the oral surgeon. This surgical intervention may also be considered for the treatment of the transsphenoidal meningoencephalocele, which also presents many problems."}
{"id": "PMID:484133", "title": "[Treatment and prognosis of the therapeutic effect of L-DOPA in dystonia musculorum deformans].", "content": "Clinico-biochemical examinations of 55 patients suffering from various clinical forms of deforming myodystonia were performed. L-DOPA was used in 47 cases. A correlation between the therapeutic effect and the level of phenylacetylglutamine excretion was revealed. The increase of the phenylacetylglutamine excretion was an evidence of a positive result of the treatment that was noted in 36 patients (77%). A resistance to the drug was observed in cases when the phenylacetylglutamine excretion did not exceed its normal level. The quantitative determination of phenylacetylglutamine in the daily portion of the urine can be used as a prognostic test of the therapeutic efficacy of L-DOPA in patients with deforming myodystonia.", "contents": "[Treatment and prognosis of the therapeutic effect of L-DOPA in dystonia musculorum deformans]. Clinico-biochemical examinations of 55 patients suffering from various clinical forms of deforming myodystonia were performed. L-DOPA was used in 47 cases. A correlation between the therapeutic effect and the level of phenylacetylglutamine excretion was revealed. The increase of the phenylacetylglutamine excretion was an evidence of a positive result of the treatment that was noted in 36 patients (77%). A resistance to the drug was observed in cases when the phenylacetylglutamine excretion did not exceed its normal level. The quantitative determination of phenylacetylglutamine in the daily portion of the urine can be used as a prognostic test of the therapeutic efficacy of L-DOPA in patients with deforming myodystonia."}
{"id": "PMID:484134", "title": "[Correction of the hormonal activity of the thyroid gland as a method of pathogenetic therapy in the complex treatment of temporal lobe epilepsy].", "content": "Corrective therapy (by thyroidin, di-iodo-thyrosine) of disturbed hormonal activity of the thyroid gland was carried out in 30 patients with temporal epilepsy. There was a favourable immediate (1,5 years) therapeutical effect: the epileptic paroxysms either disappeared or their frequency was reduced, significant normalization of the EEG and iodine metabolism, and improvement in the mental state of the patients were shown. It is recommended to include hormonal correction of the activity of the thyroid gland in the comprehensive treatment of patients with temporal epilepsy.", "contents": "[Correction of the hormonal activity of the thyroid gland as a method of pathogenetic therapy in the complex treatment of temporal lobe epilepsy]. Corrective therapy (by thyroidin, di-iodo-thyrosine) of disturbed hormonal activity of the thyroid gland was carried out in 30 patients with temporal epilepsy. There was a favourable immediate (1,5 years) therapeutical effect: the epileptic paroxysms either disappeared or their frequency was reduced, significant normalization of the EEG and iodine metabolism, and improvement in the mental state of the patients were shown. It is recommended to include hormonal correction of the activity of the thyroid gland in the comprehensive treatment of patients with temporal epilepsy."}
{"id": "PMID:484135", "title": "[Differentiated methods of treating atherosclerotic patients at the Kislovodsk health resort at early periods after transient cerebral circulatory disorders].", "content": "A dynamic examination of 135 patients with transient disorders of cerebral circulation (TDCC) was conducted in conditions of a sanatorium-resort treatment in Kislovodsk in early stages following the last attack (from 2-3 months). The method of a phasic examination and treatment was accomplished according to the following principle: polyclinic -- hospital -- resort -- polyclinic. Apart from the clinical dynamic examinations such methods as EEG, REG studies of blood coagulation and basic metabolism, and if necessary angio- and dopplerosonography were used. During their stay at the resort diadynamic currents werer used according to the method of a weak effect on the projection of spinal arteries, sinusoidal modulated currents on the cervical sympathetic nodes and plexus, carbon dioxide Narzan baths, and terrain cure. A significant improvement was seen in 10, improvement in 115, insignificant improvement in 9 patients. One patient showed no changes.", "contents": "[Differentiated methods of treating atherosclerotic patients at the Kislovodsk health resort at early periods after transient cerebral circulatory disorders]. A dynamic examination of 135 patients with transient disorders of cerebral circulation (TDCC) was conducted in conditions of a sanatorium-resort treatment in Kislovodsk in early stages following the last attack (from 2-3 months). The method of a phasic examination and treatment was accomplished according to the following principle: polyclinic -- hospital -- resort -- polyclinic. Apart from the clinical dynamic examinations such methods as EEG, REG studies of blood coagulation and basic metabolism, and if necessary angio- and dopplerosonography were used. During their stay at the resort diadynamic currents werer used according to the method of a weak effect on the projection of spinal arteries, sinusoidal modulated currents on the cervical sympathetic nodes and plexus, carbon dioxide Narzan baths, and terrain cure. A significant improvement was seen in 10, improvement in 115, insignificant improvement in 9 patients. One patient showed no changes."}
{"id": "PMID:484136", "title": "[Experience with the prognosis of strokes].", "content": "Results of using discriminant analysis for forecasting ischemic and hemorrhagic strokes are presented. Use was made of clinical data obtained from groups consisting of 400 patients with a history of ischemic strokes, 50 patients with a history of hemorrhagic strokes and 100 healthy individuals. Each observation in these groups was analyzed from 120 parameters and features. As a result of mathematical processing of these data 15 risk factors found to be the most informative for forecasting ischemic strokes, and 16 factors for forecasting hemorrhagic strokes were selected. The reliability of the identification capacity of the prognostic system was evaluated from generalized Machalanobis distance. The correctness of forecasting ischemic strokes was confirmed in 83% and of hemorrhagic strokes in 94.7% of the cases.", "contents": "[Experience with the prognosis of strokes]. Results of using discriminant analysis for forecasting ischemic and hemorrhagic strokes are presented. Use was made of clinical data obtained from groups consisting of 400 patients with a history of ischemic strokes, 50 patients with a history of hemorrhagic strokes and 100 healthy individuals. Each observation in these groups was analyzed from 120 parameters and features. As a result of mathematical processing of these data 15 risk factors found to be the most informative for forecasting ischemic strokes, and 16 factors for forecasting hemorrhagic strokes were selected. The reliability of the identification capacity of the prognostic system was evaluated from generalized Machalanobis distance. The correctness of forecasting ischemic strokes was confirmed in 83% and of hemorrhagic strokes in 94.7% of the cases."}
{"id": "PMID:484137", "title": "[Clinical picture of vibration disease caused by an overall low-frequency jerky vibration and problems involved in sanatorium treatment].", "content": "On the basis of complex clinico-physiological examinations of 102 patients suffering from vibration disease caused by general low-frequency push-type vibration the following leading symptomocomplexes were determined: vegetative polyneuritis (53%), peripheral polyneuritis (20.5%), cerebral polyneuritis (13.7%), angiodystonic syndromes and lumbosacral radiculitis (12.7%). The patients were treated with two balneopelotherapeutic complexes, such as nitrogen thermal and bromoiodine baths plus mud applications. The dynamics of the disease symptoms and of the results of additional instrumental examinations has demonstrated the efficacy of the balneopelotherapy given in sanatorium conditions, this efficacy being dependent on the disease stage, prevailing syndrome, and the therapeutic complex used.", "contents": "[Clinical picture of vibration disease caused by an overall low-frequency jerky vibration and problems involved in sanatorium treatment]. On the basis of complex clinico-physiological examinations of 102 patients suffering from vibration disease caused by general low-frequency push-type vibration the following leading symptomocomplexes were determined: vegetative polyneuritis (53%), peripheral polyneuritis (20.5%), cerebral polyneuritis (13.7%), angiodystonic syndromes and lumbosacral radiculitis (12.7%). The patients were treated with two balneopelotherapeutic complexes, such as nitrogen thermal and bromoiodine baths plus mud applications. The dynamics of the disease symptoms and of the results of additional instrumental examinations has demonstrated the efficacy of the balneopelotherapy given in sanatorium conditions, this efficacy being dependent on the disease stage, prevailing syndrome, and the therapeutic complex used."}
{"id": "PMID:484138", "title": "[Functional role of several groups of neurons in oculomotor nerve nuclei (experimental study)].", "content": "The localization and functional significance of some groups of neurons in the main nuclei of the cat aculomotor nerve were studied with the aid of the method of retrograde degeneration. The topography of the oculomotor nerve nuclei in some representatives of the order of rodents, hare-like and predatory animals was specified. The conducted study demonstrated that the cell groups in the main nuclei of the oculomotor nerve were situated rostro-caudally in the following order: for the superior straight, internal straight and inferior straight muscles. In the middle and caudal sections of the nuclei there is a semidecussation.", "contents": "[Functional role of several groups of neurons in oculomotor nerve nuclei (experimental study)]. The localization and functional significance of some groups of neurons in the main nuclei of the cat aculomotor nerve were studied with the aid of the method of retrograde degeneration. The topography of the oculomotor nerve nuclei in some representatives of the order of rodents, hare-like and predatory animals was specified. The conducted study demonstrated that the cell groups in the main nuclei of the oculomotor nerve were situated rostro-caudally in the following order: for the superior straight, internal straight and inferior straight muscles. In the middle and caudal sections of the nuclei there is a semidecussation."}
{"id": "PMID:484139", "title": "[Problem of progression in schizophrenia. I. Problem of progression of attack-like forms].", "content": "A total of 552 patients with attack-like schizophrenia, who attained old age were examined clinically. It was established that the development of negative changes in such patients occurs predominantly during the first half of life. Subsequently not all patients demonstrate a progressive development of the disease and even if it does occur it is seen in the form of more frequent or longer attacks and a complication of psychopathological disorders. At the same time there is a certain correlation between the clinical picture of the attacks and the severity of deficitary changes. A reverse development of some personality changes in long-term remissions in old age are described.", "contents": "[Problem of progression in schizophrenia. I. Problem of progression of attack-like forms]. A total of 552 patients with attack-like schizophrenia, who attained old age were examined clinically. It was established that the development of negative changes in such patients occurs predominantly during the first half of life. Subsequently not all patients demonstrate a progressive development of the disease and even if it does occur it is seen in the form of more frequent or longer attacks and a complication of psychopathological disorders. At the same time there is a certain correlation between the clinical picture of the attacks and the severity of deficitary changes. A reverse development of some personality changes in long-term remissions in old age are described."}
{"id": "PMID:484140", "title": "[Prevalence and chief variants of the course of schizophrenia with a predominance of delusional and hallucinatory-delusional disorders throughout the course of the disease (clinico-epidemiologic study)].", "content": "A nonselective group of 971 schizophrenic patients was studied. The development of the disease in these patients was characterized by the prevalence of hallucinative disorders during the whole duration of the disease. This group totalled 19.1% of the whole population of patients. The cases with continuous paranoid schizophrenia totalled 42.4% of the studied group (8.1% of the population). The rest observations formed the continual range between paranoid and attack-like schizophrenia.", "contents": "[Prevalence and chief variants of the course of schizophrenia with a predominance of delusional and hallucinatory-delusional disorders throughout the course of the disease (clinico-epidemiologic study)]. A nonselective group of 971 schizophrenic patients was studied. The development of the disease in these patients was characterized by the prevalence of hallucinative disorders during the whole duration of the disease. This group totalled 19.1% of the whole population of patients. The cases with continuous paranoid schizophrenia totalled 42.4% of the studied group (8.1% of the population). The rest observations formed the continual range between paranoid and attack-like schizophrenia."}
{"id": "PMID:484141", "title": "[Variants of the course of schizoaffective psychoses].", "content": "The clinical and follow-up studies of 107 patients with 2-10 attacks of schizoaffective psychoses permiteed one to distinguish the main variants of development: cyclothymolike, typical schizoaffective and a variant nearer to shiftlike schizophrenia. In a cyclothymolike variant, the attacks resemble phases of a cyclothymic psychoses, but the development of the disease becomes atypical dissociated. The remissions are characterized by disthymical and somatopsychic disorders and personality changes. Typical schizoaffective variants have a development with attacks, mainly of the \"acute schizophrenia\" type. Following the attacks there is a stabilization of the process with minimal personality changes. In variants nearer to shiftlike schizophrenia, the affective disorders in the framework of the attack gradually lose their intensity, while the hallucinatory delusional symptomatology acquires a tendency towards a systematization. The remissions are short with more marked personality changes.", "contents": "[Variants of the course of schizoaffective psychoses]. The clinical and follow-up studies of 107 patients with 2-10 attacks of schizoaffective psychoses permiteed one to distinguish the main variants of development: cyclothymolike, typical schizoaffective and a variant nearer to shiftlike schizophrenia. In a cyclothymolike variant, the attacks resemble phases of a cyclothymic psychoses, but the development of the disease becomes atypical dissociated. The remissions are characterized by disthymical and somatopsychic disorders and personality changes. Typical schizoaffective variants have a development with attacks, mainly of the \"acute schizophrenia\" type. Following the attacks there is a stabilization of the process with minimal personality changes. In variants nearer to shiftlike schizophrenia, the affective disorders in the framework of the attack gradually lose their intensity, while the hallucinatory delusional symptomatology acquires a tendency towards a systematization. The remissions are short with more marked personality changes."}
{"id": "PMID:484142", "title": "[Nosologic features of depression (clinico-statistical study)].", "content": "A clinico-statistical analysis of depressions with different nozological entities (125 cases) demonstrated that the nozological boundaries of endogenous and psychogenic depressions are quite obvious (if not considering the degree of the affect). Regarding the depth of affective disorders, the depressive symptomatology reflects mainly the general pathological regularities (the state of severity) and not so much the nozological traits of the disease.", "contents": "[Nosologic features of depression (clinico-statistical study)]. A clinico-statistical analysis of depressions with different nozological entities (125 cases) demonstrated that the nozological boundaries of endogenous and psychogenic depressions are quite obvious (if not considering the degree of the affect). Regarding the depth of affective disorders, the depressive symptomatology reflects mainly the general pathological regularities (the state of severity) and not so much the nozological traits of the disease."}
{"id": "PMID:484144", "title": "[Certain principles for differential utilization of metabolic treatment preparations in the complex therapy of mental disorders].", "content": "A study of 246 patients (with schizophrenia, manic depressive psychoses and psychoorganic syndrome) treated by some drugs of a metabolic action (encephalotropic\", \"nootropic\" drugs, piracetam, piriditol and pantogam) permitted one to determine the place of these preparations in a comprehensive treatment of mental disorders. On the basis of clinical studies of the above-mentioned preparations, the conclusion is made about desirability (and in some cases about necessity) of including them into pharmacotherapy. During such studies certain traits of the clinical effect in each of these drugs was examined, permitting the comparative spectra of the pharmacological activity of piracetam, piriditol and pantogam to be determined. The clinical studies of these patients indicate the necessity of a differentiated use of the mentioned drugs having in view the state of the patient and detected clinical profile of the drugs.", "contents": "[Certain principles for differential utilization of metabolic treatment preparations in the complex therapy of mental disorders]. A study of 246 patients (with schizophrenia, manic depressive psychoses and psychoorganic syndrome) treated by some drugs of a metabolic action (encephalotropic\", \"nootropic\" drugs, piracetam, piriditol and pantogam) permitted one to determine the place of these preparations in a comprehensive treatment of mental disorders. On the basis of clinical studies of the above-mentioned preparations, the conclusion is made about desirability (and in some cases about necessity) of including them into pharmacotherapy. During such studies certain traits of the clinical effect in each of these drugs was examined, permitting the comparative spectra of the pharmacological activity of piracetam, piriditol and pantogam to be determined. The clinical studies of these patients indicate the necessity of a differentiated use of the mentioned drugs having in view the state of the patient and detected clinical profile of the drugs."}
{"id": "PMID:484148", "title": "[Neurologic disorders in the late period following terminal states].", "content": "The dynamics of the psychoneurological status f 70 patients who had experienced clinical death (17 patients), massive blood loss with prolonged hypotension (30 patients), and terminal eclampsia (23 patients) was studied. In 71% of the patients various late psychoneurological disorders were revealed. Distribution of these disorders according to the WHO classification is presented. It has been found that the psychoneurological disorders of this kind may either: a) gradually disappear, b) stabilize, or c) progress. In 42% of the patients new psychoneurological disorders appeared in the late period: such a deterioration became manifest 3 to 5 months after the terminal state sustained. The discovered incompleteness of the compensation processes points to a necessity of developing a rehabilitation program.", "contents": "[Neurologic disorders in the late period following terminal states]. The dynamics of the psychoneurological status f 70 patients who had experienced clinical death (17 patients), massive blood loss with prolonged hypotension (30 patients), and terminal eclampsia (23 patients) was studied. In 71% of the patients various late psychoneurological disorders were revealed. Distribution of these disorders according to the WHO classification is presented. It has been found that the psychoneurological disorders of this kind may either: a) gradually disappear, b) stabilize, or c) progress. In 42% of the patients new psychoneurological disorders appeared in the late period: such a deterioration became manifest 3 to 5 months after the terminal state sustained. The discovered incompleteness of the compensation processes points to a necessity of developing a rehabilitation program."}
{"id": "PMID:484150", "title": "[Morphologic changes in an artery feeding an arteriovenous aneurysm over a long period following its embolization].", "content": "Different materials are used in operations for embolization of arteriovenous aneurysms on which radical operation cannot be performed. A case is described with successful embolization by means of silicon-tantulum balls of vessels supplying a giant arteriovenous aneurysm. The patient died 6 years after operation from recurrent massive hemorrhage with escape of blood into the ventricles. Morphological examination of the artery revealed neither necrotic or inflammatory changes in its walls at the site of the embolism.", "contents": "[Morphologic changes in an artery feeding an arteriovenous aneurysm over a long period following its embolization]. Different materials are used in operations for embolization of arteriovenous aneurysms on which radical operation cannot be performed. A case is described with successful embolization by means of silicon-tantulum balls of vessels supplying a giant arteriovenous aneurysm. The patient died 6 years after operation from recurrent massive hemorrhage with escape of blood into the ventricles. Morphological examination of the artery revealed neither necrotic or inflammatory changes in its walls at the site of the embolism."}
{"id": "PMID:484151", "title": "[Anatomo-experimental basis for tentorial-sinus autoplasty].", "content": "The histotoprographical features of the upper and lower sheets of the cerebellar tentorium are determined and the anatomical characteristic of their neurovascular structures is given. A method for separating the sheets of the cerebellar tentorium and forming from its upper sheet rectangular pedicled autografts facing the transverse or straight sinus, the superior petrosal sinus and the sinus drain was elaborated on human cadavers. Optimum dimensions of the temporal graft were calculated according to age and individual range of variability of the size of each half of the cerebellar tentorium. Three methods of tentorial autoplasty of the transverse sinus were used in the experiment: monograft, bigraft and total plastics of the resected sinus segment.", "contents": "[Anatomo-experimental basis for tentorial-sinus autoplasty]. The histotoprographical features of the upper and lower sheets of the cerebellar tentorium are determined and the anatomical characteristic of their neurovascular structures is given. A method for separating the sheets of the cerebellar tentorium and forming from its upper sheet rectangular pedicled autografts facing the transverse or straight sinus, the superior petrosal sinus and the sinus drain was elaborated on human cadavers. Optimum dimensions of the temporal graft were calculated according to age and individual range of variability of the size of each half of the cerebellar tentorium. Three methods of tentorial autoplasty of the transverse sinus were used in the experiment: monograft, bigraft and total plastics of the resected sinus segment."}
{"id": "PMID:484154", "title": "[Study of the strength of the adherence of the dura mater to the bones of the skull].", "content": "The strength of the fixation of the dura mater to the bones of the cranial portion and base of the skull was studied with regard to their resistance to separation. Specimens taken from individuals who had died at the age of 3 months to 4 years and from 25 to 58 years were examined. These specimens were not treated with preservatives. It was established that the strength of fixation of dura mater to the cranial bones depends on the age and individual anatomical features and is 30--1800 g/cm. The topography of the fields of minimum and maximum strength of fixation of the dura mater was determined which explains some regular features of epidural hematoma formation.", "contents": "[Study of the strength of the adherence of the dura mater to the bones of the skull]. The strength of the fixation of the dura mater to the bones of the cranial portion and base of the skull was studied with regard to their resistance to separation. Specimens taken from individuals who had died at the age of 3 months to 4 years and from 25 to 58 years were examined. These specimens were not treated with preservatives. It was established that the strength of fixation of dura mater to the cranial bones depends on the age and individual anatomical features and is 30--1800 g/cm. The topography of the fields of minimum and maximum strength of fixation of the dura mater was determined which explains some regular features of epidural hematoma formation."}
{"id": "PMID:484155", "title": "[Traumatic pneumocephalus].", "content": "Five patients with traumatic pneumocephalus are reported. A case with traumatic ventricular and subarachnoidal pneumocephalus resulting from damage to the sphenoidal sinus is described. Treatment of traumatic pneumocephalus is nonoperative is nonoperative in most cases.", "contents": "[Traumatic pneumocephalus]. Five patients with traumatic pneumocephalus are reported. A case with traumatic ventricular and subarachnoidal pneumocephalus resulting from damage to the sphenoidal sinus is described. Treatment of traumatic pneumocephalus is nonoperative is nonoperative in most cases."}
{"id": "PMID:484160", "title": "Estriol serum levels, neonatal vitality and stromal villous edema in diabetic pregnancies.", "content": "Estriol serum levels, neonatal vitality and stromal villous edema were studied in diabetic pregnancies. The average serum estriol levels in diabetic patients whose placentas had villous edema was 26.45 +/- 9.16 ng/ml. This value was significantly lower than that of the diabetic patients without villous edema (59.26 +/- 12.06 ng/ml). The average serum estriol levels in diabetic patients who gave birth to depressed newborns was 25.68 +/- 9.73 ng/ml. In diabetic women who gave birth to vigorous newborns this value was 56.57 +/- 14.10 ng/ml. The difference was statistically significant. These results and similar findings from other authors are discussed.", "contents": "Estriol serum levels, neonatal vitality and stromal villous edema in diabetic pregnancies. Estriol serum levels, neonatal vitality and stromal villous edema were studied in diabetic pregnancies. The average serum estriol levels in diabetic patients whose placentas had villous edema was 26.45 +/- 9.16 ng/ml. This value was significantly lower than that of the diabetic patients without villous edema (59.26 +/- 12.06 ng/ml). The average serum estriol levels in diabetic patients who gave birth to depressed newborns was 25.68 +/- 9.73 ng/ml. In diabetic women who gave birth to vigorous newborns this value was 56.57 +/- 14.10 ng/ml. The difference was statistically significant. These results and similar findings from other authors are discussed."}
{"id": "PMID:484161", "title": "Effect of alloxan diabetes on some aspects of lipid metabolism in albino rats.", "content": "Administration of alloxan (40 mg/kg i.v.) caused a marked decrease in the lipolytic and esterolytic activities of rat aorta. This effect was completely reversed when the diabetic rats were treated with low doses of insulin (2-3 U/die) for 3 days. The insulin-dependence of esterolysis and lipolysis in rat aorta and the role of hydrolases in atherogenesis in experimental diabetes have been discussed.", "contents": "Effect of alloxan diabetes on some aspects of lipid metabolism in albino rats. Administration of alloxan (40 mg/kg i.v.) caused a marked decrease in the lipolytic and esterolytic activities of rat aorta. This effect was completely reversed when the diabetic rats were treated with low doses of insulin (2-3 U/die) for 3 days. The insulin-dependence of esterolysis and lipolysis in rat aorta and the role of hydrolases in atherogenesis in experimental diabetes have been discussed."}
{"id": "PMID:484162", "title": "Glucose-insulin interaction in obese individuals with asymptomatic reactive hypoglycemia.", "content": "The interrelationship of glucose and insulin was investigated in obese nondiabetic subjects with asymptomatic reactive hypoglycemia. Results were compared to those obtained from obese control subjects and normal individuals. The diagnostic criteria for asymptomatic reactive hypoglycemia were the appearance of blood glucose values of 40 mg/dl and below during the postabsorptive phase of a 6-h OGTT and the absence of related symptoms. The blood glucose nadir occurred earlier in obese hypoglycemics than in obese controls. Maximum insulin response was similar in both obese groups, but occurred significantly later in obese hypoglycemics than in obese subjects without hypoglycemia and normal subjects. In obese hypoglycemics the blood glucose nadir was inversely proportional to the time of the insulin peak (i.e. the later the insulin peak the lower the blood glucose nadir) but correlated poorly to maximum insulin values. Delayed insulin response was found to be the major abnormality in asymptomatic reactive hypoglycemia and a probable cause of the decreased ability to maintain post-hyperglycemic glucose homeostasis. Decreased glucose tolerance in some obese hypoglycemics pre-treated with prednisolone suggests that asymptomatic reactive hypoglycemia could be the manifestation of an early diabetic stage.", "contents": "Glucose-insulin interaction in obese individuals with asymptomatic reactive hypoglycemia. The interrelationship of glucose and insulin was investigated in obese nondiabetic subjects with asymptomatic reactive hypoglycemia. Results were compared to those obtained from obese control subjects and normal individuals. The diagnostic criteria for asymptomatic reactive hypoglycemia were the appearance of blood glucose values of 40 mg/dl and below during the postabsorptive phase of a 6-h OGTT and the absence of related symptoms. The blood glucose nadir occurred earlier in obese hypoglycemics than in obese controls. Maximum insulin response was similar in both obese groups, but occurred significantly later in obese hypoglycemics than in obese subjects without hypoglycemia and normal subjects. In obese hypoglycemics the blood glucose nadir was inversely proportional to the time of the insulin peak (i.e. the later the insulin peak the lower the blood glucose nadir) but correlated poorly to maximum insulin values. Delayed insulin response was found to be the major abnormality in asymptomatic reactive hypoglycemia and a probable cause of the decreased ability to maintain post-hyperglycemic glucose homeostasis. Decreased glucose tolerance in some obese hypoglycemics pre-treated with prednisolone suggests that asymptomatic reactive hypoglycemia could be the manifestation of an early diabetic stage."}
{"id": "PMID:484169", "title": "Abnormal steroid sulfate in plasma of women with intrahepatic cholestasis of pregnancy.", "content": "Concentrations of numerous sulfate steroids were checked in the plasma of 10 women in normal pregnancy (NP), 21 women with intrahepatic cholestasis of pregnancy (ICP) and 10 pregnant women suffering from viral hepatitis with jaundice (VHP). A considerable increase in 5 alpha pregnane and 5 beta pregnane 3 alpha, 20 alpha diols, mono- and disulfates and in 5 alpha pregnane 3 beta, 20 alpha diols disulfates was observed only in ICP and not in VHP. This observation permits us to hypothesize that these hormone changes can have a causative role in the pathogenesis of ICP.", "contents": "Abnormal steroid sulfate in plasma of women with intrahepatic cholestasis of pregnancy. Concentrations of numerous sulfate steroids were checked in the plasma of 10 women in normal pregnancy (NP), 21 women with intrahepatic cholestasis of pregnancy (ICP) and 10 pregnant women suffering from viral hepatitis with jaundice (VHP). A considerable increase in 5 alpha pregnane and 5 beta pregnane 3 alpha, 20 alpha diols, mono- and disulfates and in 5 alpha pregnane 3 beta, 20 alpha diols disulfates was observed only in ICP and not in VHP. This observation permits us to hypothesize that these hormone changes can have a causative role in the pathogenesis of ICP."}
{"id": "PMID:484163", "title": "Effect of somatostatin (SRIF) on plasma glucose and insulin response to glucagon in liver cirrhosis.", "content": "The present study was performed in order to evaluate the plasma glucose pattern in cirrhotic patients who, in the course of a continuous somatostatin infusion (500 microgram/h), were given pulses of glucagon (1 mg i.v.). In normal as well as in cirrhotic subjects somatostatin infusion provoked a marked reduction of the IRI plasma level and this was uninfluenced by subsequent glucagon administration. The rise in plasma glucose level in response to i.v. glucagon administration during somatostatin infusion was less marked in cirrhotics compared to normal subjects. This can be attributed to a variety of factors such as reduced number of liver cells or quantitative or qualitative changes of the liver cell glucagon receptors. Glucagon does not seem to contribute to the pathogenesis of carbohydrate intolerance in liver cirrhosis.", "contents": "Effect of somatostatin (SRIF) on plasma glucose and insulin response to glucagon in liver cirrhosis. The present study was performed in order to evaluate the plasma glucose pattern in cirrhotic patients who, in the course of a continuous somatostatin infusion (500 microgram/h), were given pulses of glucagon (1 mg i.v.). In normal as well as in cirrhotic subjects somatostatin infusion provoked a marked reduction of the IRI plasma level and this was uninfluenced by subsequent glucagon administration. The rise in plasma glucose level in response to i.v. glucagon administration during somatostatin infusion was less marked in cirrhotics compared to normal subjects. This can be attributed to a variety of factors such as reduced number of liver cells or quantitative or qualitative changes of the liver cell glucagon receptors. Glucagon does not seem to contribute to the pathogenesis of carbohydrate intolerance in liver cirrhosis."}
{"id": "PMID:484170", "title": "Diverticular disease of the colon and gallstones in New Zealand and England.", "content": "The clinical presentation and subsequent treatment of 160 consecutive patients with proven diverticular disease of the colon from the Auckland Hospital, New Zealand were compared with 182 consecutive patients admitted to St. Bartholomew's Hospital, London. There were significant differences in sex incidence, in the ages of the male patients at presentation, patient symptoms, extent of disease and methods of surgical treatment. These probably reflect differences in the types of National Health Services of the two countries, rather than differences in the natural history of diverticular disease of the colon. Overall, the 342 patients studied show a very good correlation with other large series published over the last 10 years. This study confirms the previously reported association between diverticular disease of the colon and gallstones. It confirms that gallstones are more common in female patients with or without diverticular disease. It suggests that Auckland patients with gallstones are more likely to have had a cholecystectomy than London patients. The possible role of dietary fiber in the aetiology of both gallstones and diverticular disease of the colon is discussed.", "contents": "Diverticular disease of the colon and gallstones in New Zealand and England. The clinical presentation and subsequent treatment of 160 consecutive patients with proven diverticular disease of the colon from the Auckland Hospital, New Zealand were compared with 182 consecutive patients admitted to St. Bartholomew's Hospital, London. There were significant differences in sex incidence, in the ages of the male patients at presentation, patient symptoms, extent of disease and methods of surgical treatment. These probably reflect differences in the types of National Health Services of the two countries, rather than differences in the natural history of diverticular disease of the colon. Overall, the 342 patients studied show a very good correlation with other large series published over the last 10 years. This study confirms the previously reported association between diverticular disease of the colon and gallstones. It confirms that gallstones are more common in female patients with or without diverticular disease. It suggests that Auckland patients with gallstones are more likely to have had a cholecystectomy than London patients. The possible role of dietary fiber in the aetiology of both gallstones and diverticular disease of the colon is discussed."}
{"id": "PMID:484164", "title": "Effects of fat mass reduction by dieting and by lipectomy on carbohydrate metabolism in obese patients.", "content": "The interrelation of enlarged body fat mass (BFM) with reduced carbohydrate tolerance and hyperinsulinemia was studied in obese subjects with chemical diabetes. These patients were subjected to lipectomy following weight loss induced by a low-calorie, low-carbohydrate diet. An improvement in glucose tolerance and in insulin sensitivity and a reduction in insulin release during OGTT was observed after a diet-induced BFM loss of 9.9 +/-1.2 kg. Subsequent surgical reduction of BFM by 6.0 +/- 0.5 kg had no further effect upon carbohydrate tolerance, insulin release or insulin sensitivity though a marked decrease in basal plasma FFA values was observed. These findings suggest that fat mass enlargement per se has no effect on blood glucose homeostasis after oral or i.v. loading. The improvement in carbohydrate tolerance and in insulin resistance usually observed following diet-induced loss of BFM seems to be due to the reduction in calorie and carbohydrate intake rather than to decrease of BFM.", "contents": "Effects of fat mass reduction by dieting and by lipectomy on carbohydrate metabolism in obese patients. The interrelation of enlarged body fat mass (BFM) with reduced carbohydrate tolerance and hyperinsulinemia was studied in obese subjects with chemical diabetes. These patients were subjected to lipectomy following weight loss induced by a low-calorie, low-carbohydrate diet. An improvement in glucose tolerance and in insulin sensitivity and a reduction in insulin release during OGTT was observed after a diet-induced BFM loss of 9.9 +/-1.2 kg. Subsequent surgical reduction of BFM by 6.0 +/- 0.5 kg had no further effect upon carbohydrate tolerance, insulin release or insulin sensitivity though a marked decrease in basal plasma FFA values was observed. These findings suggest that fat mass enlargement per se has no effect on blood glucose homeostasis after oral or i.v. loading. The improvement in carbohydrate tolerance and in insulin resistance usually observed following diet-induced loss of BFM seems to be due to the reduction in calorie and carbohydrate intake rather than to decrease of BFM."}
{"id": "PMID:484171", "title": "Investigation of cholecystokinin-octapeptide splitting enzyme in dog kidney.", "content": "In vitro experiments were carried out to examine the enzymatic activity in various organ homogenates of the dog, which inactivates the biological activity of the synthetic cholecystokinin-octapeptide (CCK-OP). The highest splitting activity was found in the renal cortex; substantially lower activities were registered in the lung, pancreas and small intestine. It seems interesting that neither the gallbladder nor the saliva and the serum contained measurable amount of the CCK-OP splitting activity. An effort was made to characterize the CCK-OP inactivating principle found in the renal cortex. It was ascertained that the CCK-OP was inactivated by a peptidase which is heat sensitive, has a pH optimum of 7,4 and could be inhibited by chelating agents (EDTA) and epsilon-aminocaproic acid. The fact that most of the enzyme function is associated with the sediment obtained at 12 000 g speaks in favour of its mitochondrial origin.", "contents": "Investigation of cholecystokinin-octapeptide splitting enzyme in dog kidney. In vitro experiments were carried out to examine the enzymatic activity in various organ homogenates of the dog, which inactivates the biological activity of the synthetic cholecystokinin-octapeptide (CCK-OP). The highest splitting activity was found in the renal cortex; substantially lower activities were registered in the lung, pancreas and small intestine. It seems interesting that neither the gallbladder nor the saliva and the serum contained measurable amount of the CCK-OP splitting activity. An effort was made to characterize the CCK-OP inactivating principle found in the renal cortex. It was ascertained that the CCK-OP was inactivated by a peptidase which is heat sensitive, has a pH optimum of 7,4 and could be inhibited by chelating agents (EDTA) and epsilon-aminocaproic acid. The fact that most of the enzyme function is associated with the sediment obtained at 12 000 g speaks in favour of its mitochondrial origin."}
{"id": "PMID:484165", "title": "On the mechanism of the insulin-glucose interactions.", "content": "Analysis of the course of D-glucose binding to insulin has shown that the mechanism of glucose-insulin interaction is a cooperative process. Binding of D-glucose molecules to insulin is facilitated by the dissociation of insulin aggregates caused by insulin-glucose interaction. Thus, insulin behaves as a system with strong positive cooperativity. The results have been treated in accordance with theories for interactions coupled to association equilibria. The data obtained support the idea that insulin monomers are the active species responsible for insulin action.", "contents": "On the mechanism of the insulin-glucose interactions. Analysis of the course of D-glucose binding to insulin has shown that the mechanism of glucose-insulin interaction is a cooperative process. Binding of D-glucose molecules to insulin is facilitated by the dissociation of insulin aggregates caused by insulin-glucose interaction. Thus, insulin behaves as a system with strong positive cooperativity. The results have been treated in accordance with theories for interactions coupled to association equilibria. The data obtained support the idea that insulin monomers are the active species responsible for insulin action."}
{"id": "PMID:484172", "title": "Phasic and tonic types of smooth muscle activity in lower oesophageal sphincter and stomach of the dog.", "content": "Mechanical activity of isolated circular muscle strips from the lower oesophageal sphincter (LES), fundus, corpus and antrum of canine stomach was recorded. Nifedipine (10(-6) mol/l) selectively suppressed the phasic components of spontaneous, acetylcholine (ACh)- and noradrenaline (NA)-induced activity of gastric strips. The nifedipine-resistant tonic components of gastric strips were suppressed by nitroprusside sodium (NP) (10(-5) mol/l). LES strips exhibited only weak spontaneous activity. Their ACh-induced activation was nearly completely suppressed by nifedipine. Only NA was able to elicit a strong nifedipine-resistant activation which was, in contrast to the activation of fundus strips, not suppressed by NP.", "contents": "Phasic and tonic types of smooth muscle activity in lower oesophageal sphincter and stomach of the dog. Mechanical activity of isolated circular muscle strips from the lower oesophageal sphincter (LES), fundus, corpus and antrum of canine stomach was recorded. Nifedipine (10(-6) mol/l) selectively suppressed the phasic components of spontaneous, acetylcholine (ACh)- and noradrenaline (NA)-induced activity of gastric strips. The nifedipine-resistant tonic components of gastric strips were suppressed by nitroprusside sodium (NP) (10(-5) mol/l). LES strips exhibited only weak spontaneous activity. Their ACh-induced activation was nearly completely suppressed by nifedipine. Only NA was able to elicit a strong nifedipine-resistant activation which was, in contrast to the activation of fundus strips, not suppressed by NP."}
{"id": "PMID:484166", "title": "Kinetics of insulin secretion following glucose infusion in patients with atherosclerotic peripheral vascular disease.", "content": "Kinetics of insulin secretion following an i.v. glucose infusion, according to the protocol described by Cerasi and Luft, were studied in 19 patients with angiographically documented atherosclerotic peripheral vascular disease and in a group of appropriate controls without clinical signs of disease. No significant differences were noted between patients and controls in plasma cholesterol levels and in the K value following a standard i.v. glucose tolerance test. Blood glucose levels were significantly lower in the patients, whereas mean plasma insulin and triglyceride levels were significantly higher. Analysis of the glucose and insulin responses to the glucose infusion test indicated that 31.6% of the patients had a delayed and sluggish insulin response to the glucose load, fitting the criteria suggested for the diagnosis of prediabetes, versus 10% of the appropriate controls. In particular, simulation of the plasma insulin responses by a square-wave glucose stimulus, confirmed that in a significantly higher number of patients the early insulin peak was below normal limits. The results of this study suggest that increased insulin secretion is not present in patients with atherosclerotic vascular disease, in contrast to reports by other authors, and that inefficient insulin secretory mechanisms may be observed in these patients, thus possibly contributing to the development of the atherosclerotic disease.", "contents": "Kinetics of insulin secretion following glucose infusion in patients with atherosclerotic peripheral vascular disease. Kinetics of insulin secretion following an i.v. glucose infusion, according to the protocol described by Cerasi and Luft, were studied in 19 patients with angiographically documented atherosclerotic peripheral vascular disease and in a group of appropriate controls without clinical signs of disease. No significant differences were noted between patients and controls in plasma cholesterol levels and in the K value following a standard i.v. glucose tolerance test. Blood glucose levels were significantly lower in the patients, whereas mean plasma insulin and triglyceride levels were significantly higher. Analysis of the glucose and insulin responses to the glucose infusion test indicated that 31.6% of the patients had a delayed and sluggish insulin response to the glucose load, fitting the criteria suggested for the diagnosis of prediabetes, versus 10% of the appropriate controls. In particular, simulation of the plasma insulin responses by a square-wave glucose stimulus, confirmed that in a significantly higher number of patients the early insulin peak was below normal limits. The results of this study suggest that increased insulin secretion is not present in patients with atherosclerotic vascular disease, in contrast to reports by other authors, and that inefficient insulin secretory mechanisms may be observed in these patients, thus possibly contributing to the development of the atherosclerotic disease."}
{"id": "PMID:484173", "title": "Effect of gastric distension on human pancreatic secretion.", "content": "The effect of gastric distension on pancreatic secretion of bicarbonate and trypsin was studied in 10 individuals in the basal state or during stimulation with low and high doses of secretin or secretin plus cholecystokinin (CCK). Gastric distension augmented the pancreatic response to secretin but had no consistent effect on the pancreatic response to combination of secretin and CCK. We conclude that gastropancratic reflexes do not have much functional significance in man.", "contents": "Effect of gastric distension on human pancreatic secretion. The effect of gastric distension on pancreatic secretion of bicarbonate and trypsin was studied in 10 individuals in the basal state or during stimulation with low and high doses of secretin or secretin plus cholecystokinin (CCK). Gastric distension augmented the pancreatic response to secretin but had no consistent effect on the pancreatic response to combination of secretin and CCK. We conclude that gastropancratic reflexes do not have much functional significance in man."}
{"id": "PMID:484167", "title": "Lipid metabolism by muscle of diabetic rats.", "content": "This study was undertaken to ascertain whether enhanced oxidation of intracellular lipids could explain the impaired carbohydrate metabolism of diabetes. Pieces of diaphragms removed from diabetic (60--75 mg/kg streptozotocin i.v.) and control rats were incubated for 1 h with palmitate-1-14C. Tissue lipids from one piece were separated on silicic acid columns and the amount and specific activity of free fatty acids (FFA), triglycerides (TG) and phospholipids (PL) were measured. 14CO2 production was also assessed in some experiments. The other pieces of tissue were incubated for a subsequent hour (without radioactivity) at which time measurements of tissue lipid content and specific activity and 14CO2 production were again performed. FFA incorporation into CO2, tissue TG and PL was normal. TG content was moderately and PL content was slightly reduced in diabetic tissue. Changes in diaphragm TG and PL content and specific activity during the 2nd h of incubation strongly suggested that most of the 14CO2 produced during this period was derived from TG. Approximately 25% of tissue TG in both control and diabetic muscle was oxidized to CO2 during the 2nd h of incubation. In diaphragms from diabetic rats, (+)-octanoylcarnitine (an inhibitor of FFA oxidation) decreased TG oxidation considerably but had no effect on the impaired glucose uptake. Thus, these data do not support the hypothesis that the glucose-fatty acid cycle (utilizing either extra- or intracellular lipids) may account for the altered carbohydrate metabolism of diabetic muscle.", "contents": "Lipid metabolism by muscle of diabetic rats. This study was undertaken to ascertain whether enhanced oxidation of intracellular lipids could explain the impaired carbohydrate metabolism of diabetes. Pieces of diaphragms removed from diabetic (60--75 mg/kg streptozotocin i.v.) and control rats were incubated for 1 h with palmitate-1-14C. Tissue lipids from one piece were separated on silicic acid columns and the amount and specific activity of free fatty acids (FFA), triglycerides (TG) and phospholipids (PL) were measured. 14CO2 production was also assessed in some experiments. The other pieces of tissue were incubated for a subsequent hour (without radioactivity) at which time measurements of tissue lipid content and specific activity and 14CO2 production were again performed. FFA incorporation into CO2, tissue TG and PL was normal. TG content was moderately and PL content was slightly reduced in diabetic tissue. Changes in diaphragm TG and PL content and specific activity during the 2nd h of incubation strongly suggested that most of the 14CO2 produced during this period was derived from TG. Approximately 25% of tissue TG in both control and diabetic muscle was oxidized to CO2 during the 2nd h of incubation. In diaphragms from diabetic rats, (+)-octanoylcarnitine (an inhibitor of FFA oxidation) decreased TG oxidation considerably but had no effect on the impaired glucose uptake. Thus, these data do not support the hypothesis that the glucose-fatty acid cycle (utilizing either extra- or intracellular lipids) may account for the altered carbohydrate metabolism of diabetic muscle."}
{"id": "PMID:484174", "title": "Gastric stump carcinoma -- carcinogenic factors and possible preventive measures.", "content": "Cancer in the resected stomach has a very poor prognosis unless it is detected at the \"early gastric cancer\" stage. The development of gastric stump carcinoma is probably dependent on the procedure chosen for primary surgery (resection). Local irritation accompanying the execution of anastomosis is also discussed as a possible cause of malignant transformation. Surgical precautions should include the choice of methods avoiding reflux, and the use of resorbable suture material for performing an exact and well-matched anastomosis. Periodic postoperative control is essential; in addition to radiological methods, endoscopy and biopsy are indicated for adequate identification of local abnormalities and mucosal changes. Patients whose high risk was stated in previous examinations, must be re-examined at shorter intervals. Identification of changes at the early cancer stage requires close cooperation of surgeon and pathologist.", "contents": "Gastric stump carcinoma -- carcinogenic factors and possible preventive measures. Cancer in the resected stomach has a very poor prognosis unless it is detected at the \"early gastric cancer\" stage. The development of gastric stump carcinoma is probably dependent on the procedure chosen for primary surgery (resection). Local irritation accompanying the execution of anastomosis is also discussed as a possible cause of malignant transformation. Surgical precautions should include the choice of methods avoiding reflux, and the use of resorbable suture material for performing an exact and well-matched anastomosis. Periodic postoperative control is essential; in addition to radiological methods, endoscopy and biopsy are indicated for adequate identification of local abnormalities and mucosal changes. Patients whose high risk was stated in previous examinations, must be re-examined at shorter intervals. Identification of changes at the early cancer stage requires close cooperation of surgeon and pathologist."}
{"id": "PMID:484175", "title": "Hereditary pancreatitis: morphological pictures (ERCP) in the youngest member of a family.", "content": "Endoscopic retrograde cholangiopancreatography (ERCP) pictures of the pancreatic duct and of the biliary tract are described in the youngest member of a family with hereditary pancreatitis. The endoscopic changes are very similar to those usually observed in patients affected by chronic relapsing pancreatitis. Morphological changes were particularly marked in the body and tail of the pancreas. However, no abnormal changes were observed in the biliary tract.", "contents": "Hereditary pancreatitis: morphological pictures (ERCP) in the youngest member of a family. Endoscopic retrograde cholangiopancreatography (ERCP) pictures of the pancreatic duct and of the biliary tract are described in the youngest member of a family with hereditary pancreatitis. The endoscopic changes are very similar to those usually observed in patients affected by chronic relapsing pancreatitis. Morphological changes were particularly marked in the body and tail of the pancreas. However, no abnormal changes were observed in the biliary tract."}
{"id": "PMID:484177", "title": "Peak expiratory flow during a passive exhalation: an index of expiratory resistance during anesthesia.", "content": "During a passive exhalation performed between (FRC + VT) and FRC, peak expiratory flow (VmaxE) varies as a function of the respiratory system expiratory resistance, RT, with the following relationship: VmaxE = VT/(RT . CT), where Cr is the total respiratory system compliance (static). This expression assumes: (1) a laminar flow regimen during expiration, and (2) a constant CT value over the range of VT. Both these conditions are usually attained during controlled ventilation in the apneic patient. VmaxE can be monitored continuously with relative ease, and may represent a simple and useful index of the mechanical properties of the respiratory system in the anesthetized patient.", "contents": "Peak expiratory flow during a passive exhalation: an index of expiratory resistance during anesthesia. During a passive exhalation performed between (FRC + VT) and FRC, peak expiratory flow (VmaxE) varies as a function of the respiratory system expiratory resistance, RT, with the following relationship: VmaxE = VT/(RT . CT), where Cr is the total respiratory system compliance (static). This expression assumes: (1) a laminar flow regimen during expiration, and (2) a constant CT value over the range of VT. Both these conditions are usually attained during controlled ventilation in the apneic patient. VmaxE can be monitored continuously with relative ease, and may represent a simple and useful index of the mechanical properties of the respiratory system in the anesthetized patient."}
{"id": "PMID:484178", "title": "Long- and short-term mortality rates in patients who primarily survive cardiac arrest compared with a normal population.", "content": "This is a follow-up study of 180 survivors after cardiac arrest outside intensive care and coronary care units. The follow-up extended over 0.5 to 8.5 years (averaged 4.3 years) after the primary cardiac arrest. Of the patients, 72 (40%) were discharged from hospital, 13 with anoxic brain damage. Thirty-ourred in 43% after 2 years, and in 50% after 3 years. After that time, the mortality was similar to that of a comparable normal population. At the end of the study, 34 patients were still alive, including eight with neurological sequelae. Their present cardiac function was satisfactory in the majority (59%) of the patients falling into group I or II of the American Heart Association classification. Their social situation, however, left much to be desired, as only 50% had fully or partly regained their previous level of activity.", "contents": "Long- and short-term mortality rates in patients who primarily survive cardiac arrest compared with a normal population. This is a follow-up study of 180 survivors after cardiac arrest outside intensive care and coronary care units. The follow-up extended over 0.5 to 8.5 years (averaged 4.3 years) after the primary cardiac arrest. Of the patients, 72 (40%) were discharged from hospital, 13 with anoxic brain damage. Thirty-ourred in 43% after 2 years, and in 50% after 3 years. After that time, the mortality was similar to that of a comparable normal population. At the end of the study, 34 patients were still alive, including eight with neurological sequelae. Their present cardiac function was satisfactory in the majority (59%) of the patients falling into group I or II of the American Heart Association classification. Their social situation, however, left much to be desired, as only 50% had fully or partly regained their previous level of activity."}
{"id": "PMID:484179", "title": "Studies on muscle relaxants during haemodialysis.", "content": "Signs of neuromuscular block were evident more than 20 hours after the administration of alcuronium to an anuric patient. Complete recovery occurred during haemodialysis. We therefore decided to study the dialysance of three radioactive non-depolarising relaxants during haemodialysis of four patients with chronic renal failure. Although dimethyl tubocurarine and alcuronium were equal as regards dialysance, the concentration of the former, in plasma, falls faster than does alcuronium. It is believed that a larger volume of distribution occurs with dimethyl tubocurarine. In spite of the fact that the dialysance of muscle relaxants is small, haemodialysis might lower the concentration of these substances in the plasma to a level below the critical point which produces paralysis.", "contents": "Studies on muscle relaxants during haemodialysis. Signs of neuromuscular block were evident more than 20 hours after the administration of alcuronium to an anuric patient. Complete recovery occurred during haemodialysis. We therefore decided to study the dialysance of three radioactive non-depolarising relaxants during haemodialysis of four patients with chronic renal failure. Although dimethyl tubocurarine and alcuronium were equal as regards dialysance, the concentration of the former, in plasma, falls faster than does alcuronium. It is believed that a larger volume of distribution occurs with dimethyl tubocurarine. In spite of the fact that the dialysance of muscle relaxants is small, haemodialysis might lower the concentration of these substances in the plasma to a level below the critical point which produces paralysis."}
{"id": "PMID:484180", "title": "Importance of using proper techniques to teach cardiopulmonary resuscitation to laymen.", "content": "Immediate attempts to resuscitate patients with cardiac arrest by lay bystanders distinctly increase the chances of survival in such patients. In the present study we investigated possibilities of intensifying the cardiopulmonary resuscitation (CPR) training of laymen. A group of 46 young conscripts (test group) was given 2 hours of CPR instruction in what was considered the most efficient way. The procedures included showing a film on the theory and practice of CPR, a discussion of the film, and then 3 to 5 min instructed individual practice with a recording manikin. Another 56 conscripts participated in a 1-h illustrated lecture on CPR, which was followed by group training with a non-recording manikin; they served as a reference group. The performance of the subjects in CPR was measured with a recording manikin before and 2 months after the training. Before training, none of the subjects passed the surprise resuscitation test. After training, the skills of 62% of the subjects in the test group were acceptable according to original criteria for lay public, and 28% passed the test according to the strict criteria for medical personnel. The less intensive training given to the reference group did not improve the acceptability of resuscitation. It is concluded that CPR training for laymen may lead to failure to attain an adequate level of skill. Proper training techniques and equipment are important, but even their use does not guarantee that an adequate level of CPR performance will be attained by all laymen if their skills are not later refreshed.", "contents": "Importance of using proper techniques to teach cardiopulmonary resuscitation to laymen. Immediate attempts to resuscitate patients with cardiac arrest by lay bystanders distinctly increase the chances of survival in such patients. In the present study we investigated possibilities of intensifying the cardiopulmonary resuscitation (CPR) training of laymen. A group of 46 young conscripts (test group) was given 2 hours of CPR instruction in what was considered the most efficient way. The procedures included showing a film on the theory and practice of CPR, a discussion of the film, and then 3 to 5 min instructed individual practice with a recording manikin. Another 56 conscripts participated in a 1-h illustrated lecture on CPR, which was followed by group training with a non-recording manikin; they served as a reference group. The performance of the subjects in CPR was measured with a recording manikin before and 2 months after the training. Before training, none of the subjects passed the surprise resuscitation test. After training, the skills of 62% of the subjects in the test group were acceptable according to original criteria for lay public, and 28% passed the test according to the strict criteria for medical personnel. The less intensive training given to the reference group did not improve the acceptability of resuscitation. It is concluded that CPR training for laymen may lead to failure to attain an adequate level of skill. Proper training techniques and equipment are important, but even their use does not guarantee that an adequate level of CPR performance will be attained by all laymen if their skills are not later refreshed."}
{"id": "PMID:484181", "title": "Regional lung function following hip arthroplasty and preoperative normovolemic hemodilution.", "content": "The effect of preoperative normovolemic hemodilution with dextran 70 as dilutional agent on postoperative regional lung function was evaluated in patients undergoing hip arthroplasty in the lateral decubitus position. The major part of the surgical blood loss of these patients was replaced with the received autologous blood. The results obtained were compared to changes in a group of patients in whom the operative bleeding was replaced with bank blood. Regional lung function, as studied with Xe133 radiospirometry, is not negatively influenced by the hemodilution technique. In both groups a reduction in perfusion, ventilation and volume of ventilated alveoli was revealed postoperatively in the lung that was dependent during surgery, probably caused by impaired ventilation of this lung and peroperatively induced microembolism. A more rapid normalization was found in the preoperatively hemodiluted patients. The reduced volume of bank blood transfused to these patients and/or a protective effect of the preoperatively infused dextran on the microembolism evoked by the surgical trauma might explain this difference between the groups studied.", "contents": "Regional lung function following hip arthroplasty and preoperative normovolemic hemodilution. The effect of preoperative normovolemic hemodilution with dextran 70 as dilutional agent on postoperative regional lung function was evaluated in patients undergoing hip arthroplasty in the lateral decubitus position. The major part of the surgical blood loss of these patients was replaced with the received autologous blood. The results obtained were compared to changes in a group of patients in whom the operative bleeding was replaced with bank blood. Regional lung function, as studied with Xe133 radiospirometry, is not negatively influenced by the hemodilution technique. In both groups a reduction in perfusion, ventilation and volume of ventilated alveoli was revealed postoperatively in the lung that was dependent during surgery, probably caused by impaired ventilation of this lung and peroperatively induced microembolism. A more rapid normalization was found in the preoperatively hemodiluted patients. The reduced volume of bank blood transfused to these patients and/or a protective effect of the preoperatively infused dextran on the microembolism evoked by the surgical trauma might explain this difference between the groups studied."}
{"id": "PMID:484182", "title": "Influence of general anaesthesia on portal pressure in liver cirrhosis and portal hypertension.", "content": "In 19 patients with cirrhosis of the liver and portal hypertension, a catheter was inserted into the portal vein using a percutaneous transhepatic technique. The portal pressure was measured during general anaesthesia with and without halothane, and in the awake state. Addition of halothane to the N2O:O2 anaesthesia did not change portal venous pressure, in spite of a significant fall in arterial blood pressure. Portal venous pressure under general anaesthesia with complete muscle relaxation did not differ from the pressure in the resting, awake patient.", "contents": "Influence of general anaesthesia on portal pressure in liver cirrhosis and portal hypertension. In 19 patients with cirrhosis of the liver and portal hypertension, a catheter was inserted into the portal vein using a percutaneous transhepatic technique. The portal pressure was measured during general anaesthesia with and without halothane, and in the awake state. Addition of halothane to the N2O:O2 anaesthesia did not change portal venous pressure, in spite of a significant fall in arterial blood pressure. Portal venous pressure under general anaesthesia with complete muscle relaxation did not differ from the pressure in the resting, awake patient."}
{"id": "PMID:484183", "title": "Epidural analgesia inhibits the renin and aldosterone response to surgery.", "content": "Renin activity and aldosterone concentration in plasma and excretion of sodium and potassium in urine were measured during a period of 24 hours in 12 patients undergoing hysterectomy under general anaesthesia or epidural analgesia. Analgesia extended from T4 to S5 and was effective throughout the study. The normal stress-induced increase in plasma renin activity and aldosterone was inhibited by epidural analgesia. Urinary excretion of potassium was significantly lower in the epidural group, but sodium and water retention showed no difference between groups. It is concluded that neurogenic stimuli from the surgical area are important release mechanisms of the renin-aldosterone response to surgery. The results suggest that post-operative sodium retention is caused by factors other than the mineralocorticoid system.", "contents": "Epidural analgesia inhibits the renin and aldosterone response to surgery. Renin activity and aldosterone concentration in plasma and excretion of sodium and potassium in urine were measured during a period of 24 hours in 12 patients undergoing hysterectomy under general anaesthesia or epidural analgesia. Analgesia extended from T4 to S5 and was effective throughout the study. The normal stress-induced increase in plasma renin activity and aldosterone was inhibited by epidural analgesia. Urinary excretion of potassium was significantly lower in the epidural group, but sodium and water retention showed no difference between groups. It is concluded that neurogenic stimuli from the surgical area are important release mechanisms of the renin-aldosterone response to surgery. The results suggest that post-operative sodium retention is caused by factors other than the mineralocorticoid system."}
{"id": "PMID:484184", "title": "Increased serum concentrations of lactic, pyruvic and uric acid and bilibubin after postoperative xylitol infusion.", "content": "Xylitol has been suggested as a more advantageous calory source for intravenous administration than glucose in certain clinical situation, but the general suitability of intravenous xylitol infusion has not been confirmed. Thirty-middle-aged women were infused with 100 g of xylitol as postoperative fluid therapy after gynaecological laparotomy and general anaesthesia. Another 10 women received 50 g of glucose in a similar manner and served as a reference group. Infusion of xylitol both at the rate of 0.25 g/kg/h (1000 ml 10% xylitol in approx. 8 h) and 0.5 g/kg/h (1000 ml 10% xylitol in approx. 4 h) caused a distinct increase in the serum concentrations of lactic acid, pyruvic acid, and uric acid; such an increase was not seen with glucose infusion. The faster infusion of xylitol also distinctly increased serum bilirubin concentrations. Because of the possibility of lactic acidosis and urate deposits in kidneys, infusion of 100 g or more of xylitol at a rate of 0.25 g/kg/h or faster is not safe for postoperative fluid therapy in routine clinical work.", "contents": "Increased serum concentrations of lactic, pyruvic and uric acid and bilibubin after postoperative xylitol infusion. Xylitol has been suggested as a more advantageous calory source for intravenous administration than glucose in certain clinical situation, but the general suitability of intravenous xylitol infusion has not been confirmed. Thirty-middle-aged women were infused with 100 g of xylitol as postoperative fluid therapy after gynaecological laparotomy and general anaesthesia. Another 10 women received 50 g of glucose in a similar manner and served as a reference group. Infusion of xylitol both at the rate of 0.25 g/kg/h (1000 ml 10% xylitol in approx. 8 h) and 0.5 g/kg/h (1000 ml 10% xylitol in approx. 4 h) caused a distinct increase in the serum concentrations of lactic acid, pyruvic acid, and uric acid; such an increase was not seen with glucose infusion. The faster infusion of xylitol also distinctly increased serum bilirubin concentrations. Because of the possibility of lactic acidosis and urate deposits in kidneys, infusion of 100 g or more of xylitol at a rate of 0.25 g/kg/h or faster is not safe for postoperative fluid therapy in routine clinical work."}
{"id": "PMID:484185", "title": "Effect of different anesthetics on the pharmacokinetics and pharmacodynamics of pancuronium in the cat.", "content": "To investigate the effect of different anesthetics on the pharmacokinetics and pharmacodynamics of pancuronium, 120 microgram/kg i.v., cats were anesthetized with either pentobarbital (N = 4), ketamine (N = 4), enflurane (N = 5), or halothane (N = 5). A longer onset time and duration of neuromuscular blockade occurred during enflurane and halothane anesthesia. The apparent elimination half-life was longer and the total voluem of distribution at steady state larger during halothane anesthesia. The plasma concentration of pancuronium required for neuromuscular blockades was less during enflurane than during the other three anesthetics. We conclude that inhalation anesthetics may prolong a neuromuscular blockade by altering both the pharmacokinetics and pharmacodynamics of pancuronium.", "contents": "Effect of different anesthetics on the pharmacokinetics and pharmacodynamics of pancuronium in the cat. To investigate the effect of different anesthetics on the pharmacokinetics and pharmacodynamics of pancuronium, 120 microgram/kg i.v., cats were anesthetized with either pentobarbital (N = 4), ketamine (N = 4), enflurane (N = 5), or halothane (N = 5). A longer onset time and duration of neuromuscular blockade occurred during enflurane and halothane anesthesia. The apparent elimination half-life was longer and the total voluem of distribution at steady state larger during halothane anesthesia. The plasma concentration of pancuronium required for neuromuscular blockades was less during enflurane than during the other three anesthetics. We conclude that inhalation anesthetics may prolong a neuromuscular blockade by altering both the pharmacokinetics and pharmacodynamics of pancuronium."}
{"id": "PMID:484186", "title": "Studies on intrapulmonary gas distribution in the normal subject. Influence of anaesthesia and artificial ventilation.", "content": "Intrapulmonary gas distribution, assessed by a multiple breath, nitrogen washout technique and expressed by the Fowler index NWOD, was studied in subjects with healthy lungs during spontaneous breathing while awake, and during mechanical ventilation under halothane anaesthesia. The distribution index rose from a mean of 32% awake to 56% during anaesthesia. An attempt was also made to differentiate between the contribution of gravitational (airway closure) and non-gravitational (diffuse airway obstruction) inhomogeneity of ventilation. This was accomplished by measurement of the slope of the alveolar plateau during a sinle breath nitrogen washout. The slope was similar awake and anaesthetized, and it increased equally under both conditions, when preinspiratory lung volume was stepped up from RV to CC, CC being the lung volume at which airways begin to close during expiration. NWOD was significantly correlated to the degree of airway closure, expressed as FRC-CC, and, less so, to inspiratory resistance. It is suggested that a less efficient ventilation distribution in anaesthetized normal subjects, as measured by NWOD, is caused rather by airway closure (gravitational inhomogeneity) than by diffuse airway obstruction (non-gravitational inhomogeneity of ventilation).", "contents": "Studies on intrapulmonary gas distribution in the normal subject. Influence of anaesthesia and artificial ventilation. Intrapulmonary gas distribution, assessed by a multiple breath, nitrogen washout technique and expressed by the Fowler index NWOD, was studied in subjects with healthy lungs during spontaneous breathing while awake, and during mechanical ventilation under halothane anaesthesia. The distribution index rose from a mean of 32% awake to 56% during anaesthesia. An attempt was also made to differentiate between the contribution of gravitational (airway closure) and non-gravitational (diffuse airway obstruction) inhomogeneity of ventilation. This was accomplished by measurement of the slope of the alveolar plateau during a sinle breath nitrogen washout. The slope was similar awake and anaesthetized, and it increased equally under both conditions, when preinspiratory lung volume was stepped up from RV to CC, CC being the lung volume at which airways begin to close during expiration. NWOD was significantly correlated to the degree of airway closure, expressed as FRC-CC, and, less so, to inspiratory resistance. It is suggested that a less efficient ventilation distribution in anaesthetized normal subjects, as measured by NWOD, is caused rather by airway closure (gravitational inhomogeneity) than by diffuse airway obstruction (non-gravitational inhomogeneity of ventilation)."}
{"id": "PMID:484187", "title": "Pancuronium bromide precurarisation. II An evaluation of clinical aspects in patients of female sex.", "content": "The authors have studied the effects of precurarisation by means of pancuronium on 120 female patients by using three multiple doses of pancuronium bromide. The authors have demonstrated that the dose of pancuronium, capable of abolishing almost completely fasciculations and postoperative succinylcholine myalgias, corresponds to 0.009 mg/kg. This dose has proved to be most efficient in causing a considerable increase in subjective precurarisation symptoms (Heavy eye-lids, blurred vision etc.). Such symptoms have never caused even the least discomfort to the patients. The authors have finally demonstrated that the doses of pancuronium employed cause a decrease in V.C. within limits that do not, however, compromise ventilation.", "contents": "Pancuronium bromide precurarisation. II An evaluation of clinical aspects in patients of female sex. The authors have studied the effects of precurarisation by means of pancuronium on 120 female patients by using three multiple doses of pancuronium bromide. The authors have demonstrated that the dose of pancuronium, capable of abolishing almost completely fasciculations and postoperative succinylcholine myalgias, corresponds to 0.009 mg/kg. This dose has proved to be most efficient in causing a considerable increase in subjective precurarisation symptoms (Heavy eye-lids, blurred vision etc.). Such symptoms have never caused even the least discomfort to the patients. The authors have finally demonstrated that the doses of pancuronium employed cause a decrease in V.C. within limits that do not, however, compromise ventilation."}
{"id": "PMID:484188", "title": "Asymptomatic myasthenia gravis manifested after removal of mediastinal tumors. Report of two cases.", "content": "Asymptomatic myasthenia gravis firstly manifested as total paralysis in patients submitted to a surgical procedure should be considered extremely rare. Two cases of latent myasthenia gravis manifested after thoracotomy for removal of mediastinal tumor are reported. Prolonged apnoea and need for postoperative mechanical support of respiration was most likely due to non-depolarizing relaxants administration. In both cases edrophonium test was positive and the histology of the tumor showed thymoma. The further recovery after pyridostigmine treatment was uneventful for both patients.", "contents": "Asymptomatic myasthenia gravis manifested after removal of mediastinal tumors. Report of two cases. Asymptomatic myasthenia gravis firstly manifested as total paralysis in patients submitted to a surgical procedure should be considered extremely rare. Two cases of latent myasthenia gravis manifested after thoracotomy for removal of mediastinal tumor are reported. Prolonged apnoea and need for postoperative mechanical support of respiration was most likely due to non-depolarizing relaxants administration. In both cases edrophonium test was positive and the histology of the tumor showed thymoma. The further recovery after pyridostigmine treatment was uneventful for both patients."}
{"id": "PMID:484189", "title": "A premature referral to the mortuary. Cerebral recovery with barbiturate therapy.", "content": "The authors report a patient in circulatory arrest with unusual spontaneous cardiopulmonary recovery in the mortuary. Cerebral outcome and clinical evaluation of various therapeutic modalities in postischemic encephalopathy are discussed briefly.", "contents": "A premature referral to the mortuary. Cerebral recovery with barbiturate therapy. The authors report a patient in circulatory arrest with unusual spontaneous cardiopulmonary recovery in the mortuary. Cerebral outcome and clinical evaluation of various therapeutic modalities in postischemic encephalopathy are discussed briefly."}
{"id": "PMID:484191", "title": "[Hematolymphatic transport in skin, subcutaneous tissue and superficial lamina of the fascia of the lower leg in varicose veins].", "content": "The lymphatic bed of the skin and subcutaneous tissues of the lower leg from 20 operated patients was studied by light and electron microscopy and by phlebographic methods. Three stages of development of the disease were examined: without complicated form of varicose veins, complicated form, and postthrombophlebitic syndrome. Morphological features of the state of the lymphatic bed of the skin, subcutaneous connective tissue and fascia of the lower leg in the initial stage of disease show the fine structure changes of lymphatic vessels and capillary walls interpreted as a compensation phenomenon. It seems that the structure alterations of endotheliocytes of lymphatic capillaries and the connective tissue surrounding them found in this study in the complicated form of the disease and the postthrombophlebitic syndrome are the basis of the mechanism of transport-resorption insufficiency of the lymph vessels' terminal flow paths.", "contents": "[Hematolymphatic transport in skin, subcutaneous tissue and superficial lamina of the fascia of the lower leg in varicose veins]. The lymphatic bed of the skin and subcutaneous tissues of the lower leg from 20 operated patients was studied by light and electron microscopy and by phlebographic methods. Three stages of development of the disease were examined: without complicated form of varicose veins, complicated form, and postthrombophlebitic syndrome. Morphological features of the state of the lymphatic bed of the skin, subcutaneous connective tissue and fascia of the lower leg in the initial stage of disease show the fine structure changes of lymphatic vessels and capillary walls interpreted as a compensation phenomenon. It seems that the structure alterations of endotheliocytes of lymphatic capillaries and the connective tissue surrounding them found in this study in the complicated form of the disease and the postthrombophlebitic syndrome are the basis of the mechanism of transport-resorption insufficiency of the lymph vessels' terminal flow paths."}
{"id": "PMID:484192", "title": "[Anterior falcate artery in adults: histology of the relation of superior sagittal sinus, dural vein and the arachnoid granulations].", "content": "The anterior falcate artery, which is the continuation of the anterior ethmoidal, supplies the dura mater in the region of the superior sagittal sinus as far almost as the coronal suture. Graphic reconstructions show its relationships to the veins, the sinus, and the arachnoid granulations. Histological studies of the artery emphasize its adaptation to longitudinal stretching. The complexity of its relationships suggests a functional significance beyond that of nourishing the dura.", "contents": "[Anterior falcate artery in adults: histology of the relation of superior sagittal sinus, dural vein and the arachnoid granulations]. The anterior falcate artery, which is the continuation of the anterior ethmoidal, supplies the dura mater in the region of the superior sagittal sinus as far almost as the coronal suture. Graphic reconstructions show its relationships to the veins, the sinus, and the arachnoid granulations. Histological studies of the artery emphasize its adaptation to longitudinal stretching. The complexity of its relationships suggests a functional significance beyond that of nourishing the dura."}
{"id": "PMID:484193", "title": "Anatomical closure of the ductus arteriosus in the dog (Canis familiaris).", "content": "The ductus arteriosus was studied in 138 dogs (80 female and 58 male) aged from 6 h to 21 days. This experiment led us to conclude that the anatomical closure of the d. arteriosus starts within 96 h and is obliterated after 6 days in 100% of the animals studied. We have also evidenced that the closure starts in the medial part of the d. arteriosus and progresses to the pulmonary artery and aorta.", "contents": "Anatomical closure of the ductus arteriosus in the dog (Canis familiaris). The ductus arteriosus was studied in 138 dogs (80 female and 58 male) aged from 6 h to 21 days. This experiment led us to conclude that the anatomical closure of the d. arteriosus starts within 96 h and is obliterated after 6 days in 100% of the animals studied. We have also evidenced that the closure starts in the medial part of the d. arteriosus and progresses to the pulmonary artery and aorta."}
{"id": "PMID:484194", "title": "Cellular thorn-like projections in human endocervical epithelium. Preliminary scanning electron-microscopic study.", "content": "By means of scanning electron-microscopic investigations, thorn-like projections (TLP) were observed in the cilio-secretory epithelium of the ventral surface of the human endocervix. These projections, which seem to be characteristic of a new cell type, were seen at different stages of the menstrual cycle (days 8, 14 and 21) in 4 apparently healthy fertile women. Striking differences in length, with a maximum size at midcycle, suggest an evolutive process throughout the menstrual cycle. The origin and possible physiological role of TLP in the reproductive process are discussed.", "contents": "Cellular thorn-like projections in human endocervical epithelium. Preliminary scanning electron-microscopic study. By means of scanning electron-microscopic investigations, thorn-like projections (TLP) were observed in the cilio-secretory epithelium of the ventral surface of the human endocervix. These projections, which seem to be characteristic of a new cell type, were seen at different stages of the menstrual cycle (days 8, 14 and 21) in 4 apparently healthy fertile women. Striking differences in length, with a maximum size at midcycle, suggest an evolutive process throughout the menstrual cycle. The origin and possible physiological role of TLP in the reproductive process are discussed."}
{"id": "PMID:484195", "title": "Aging in retinas and optic nerves of 2.5- to 9-month-old mice.", "content": "Aging of the retina and optic nerve was studied in 2.5- to 9-month-old albino mice. A change was found in the number of visual cells and in the distribution of large versus small optic nerve fibers. No significant change was found in the number of retinal phagosomes and in the total number of optic nerve fibers.", "contents": "Aging in retinas and optic nerves of 2.5- to 9-month-old mice. Aging of the retina and optic nerve was studied in 2.5- to 9-month-old albino mice. A change was found in the number of visual cells and in the distribution of large versus small optic nerve fibers. No significant change was found in the number of retinal phagosomes and in the total number of optic nerve fibers."}
{"id": "PMID:484196", "title": "Delayed maturation of the male cerebral cortex in rats.", "content": "45 male and female Wistar rats were given a single injection of 3H-thymidine (10 mu Ci/g body weight) on day 1, 7, 14 or 21. All animals survived until 60 days of age when they were perfused with 10% neutral formalin and the brains were removed and prepared for autoradiography. The sagittal section of the cortex (L980 micron) was 6.8% larger in the males (p less than 0.05) but the packing density of the cortical cells was 5.9% higher in the females (p less than 0.01), thus bringing the total number of cells to the male levels. The diameter of the female cortical cells was 3.8% smaller than those of the males (p less than 0.05). The greatest difference was among the smaller cells (3-9 micron). The rate of postnatal acquisition of cortical cells was indicated by the number of radioactive-labelled cells. Males had more labeled cells after each injection; it was most pronounced (32% difference) on day 7 (p less than 0.05). This may reflect a delayed acquisition rate of cells formed before birth, since more cells could be labeled by the postnatal injection.", "contents": "Delayed maturation of the male cerebral cortex in rats. 45 male and female Wistar rats were given a single injection of 3H-thymidine (10 mu Ci/g body weight) on day 1, 7, 14 or 21. All animals survived until 60 days of age when they were perfused with 10% neutral formalin and the brains were removed and prepared for autoradiography. The sagittal section of the cortex (L980 micron) was 6.8% larger in the males (p less than 0.05) but the packing density of the cortical cells was 5.9% higher in the females (p less than 0.01), thus bringing the total number of cells to the male levels. The diameter of the female cortical cells was 3.8% smaller than those of the males (p less than 0.05). The greatest difference was among the smaller cells (3-9 micron). The rate of postnatal acquisition of cortical cells was indicated by the number of radioactive-labelled cells. Males had more labeled cells after each injection; it was most pronounced (32% difference) on day 7 (p less than 0.05). This may reflect a delayed acquisition rate of cells formed before birth, since more cells could be labeled by the postnatal injection."}
{"id": "PMID:484197", "title": "Interneuronal synapses in the local ganglia of the cat urinary bladder.", "content": "The interneuronal synapses of the urinary bladder in the cat were studied by electron microscopy. The great majority of the fibres containing vesicles are found within the ganglia occurring in the trigonum area. Morphologically differentiated synaptic contacts could be observed on the surface of the local neurons and between the different nerve processes. The presynaptic terminals can be divided into three types based on a combination of synaptic vesicles. Type I terminals, presumably cholinergic synaptic terminals, contain only small clear vesicles of 40-50 nm in diameter. Type II terminals, presumably adrenergic terminals, are characterized by small granulated vesicles of 40-60 nm in diameter. Type III terminals, probably of local origin, contain a variable number of large granulated vesicles of 80-140 nm in diameter. Occasionally, a single nerve fibre contacted several (two or four) other nerve processes forming a typical synapse. In other cases, on one nerve cell soma or on other nerve processes there are two or three different-type nerve terminals establishing synapses. It might be inferred from these observations that convergence and divergence can occur in the local ganglia and that cholinergic and adrenergic synaptic terminals can modulate the ganglionic activity. However, a local circuit also can play an important role in coordinating the function of the bladder.", "contents": "Interneuronal synapses in the local ganglia of the cat urinary bladder. The interneuronal synapses of the urinary bladder in the cat were studied by electron microscopy. The great majority of the fibres containing vesicles are found within the ganglia occurring in the trigonum area. Morphologically differentiated synaptic contacts could be observed on the surface of the local neurons and between the different nerve processes. The presynaptic terminals can be divided into three types based on a combination of synaptic vesicles. Type I terminals, presumably cholinergic synaptic terminals, contain only small clear vesicles of 40-50 nm in diameter. Type II terminals, presumably adrenergic terminals, are characterized by small granulated vesicles of 40-60 nm in diameter. Type III terminals, probably of local origin, contain a variable number of large granulated vesicles of 80-140 nm in diameter. Occasionally, a single nerve fibre contacted several (two or four) other nerve processes forming a typical synapse. In other cases, on one nerve cell soma or on other nerve processes there are two or three different-type nerve terminals establishing synapses. It might be inferred from these observations that convergence and divergence can occur in the local ganglia and that cholinergic and adrenergic synaptic terminals can modulate the ganglionic activity. However, a local circuit also can play an important role in coordinating the function of the bladder."}
{"id": "PMID:484198", "title": "Asymmetry in the skull.", "content": "Various linear and angular measurements were taken in 118 normal skulls of adult humans. The two halves of the skulls were found to be symmetrical. The phenomenon of asymmetry of various parts of the body, in relation to cerebral dominance and specific skills has been discussed in view of the findings of the study and in the light of the available literature.", "contents": "Asymmetry in the skull. Various linear and angular measurements were taken in 118 normal skulls of adult humans. The two halves of the skulls were found to be symmetrical. The phenomenon of asymmetry of various parts of the body, in relation to cerebral dominance and specific skills has been discussed in view of the findings of the study and in the light of the available literature."}
{"id": "PMID:484199", "title": "Absence of the mental foramen in dry human mandibles.", "content": "The absence of the mental foramen was investigated in 1,435 dry human mandibles (2,870 sides). The foramen was absent twice in the right side (0.06%) and once in the left side (0.03%). The meaning of this anatomical variation was commented.", "contents": "Absence of the mental foramen in dry human mandibles. The absence of the mental foramen was investigated in 1,435 dry human mandibles (2,870 sides). The foramen was absent twice in the right side (0.06%) and once in the left side (0.03%). The meaning of this anatomical variation was commented."}
{"id": "PMID:484200", "title": "Teratogenic effects of a single oral administration of methylmercuric chloride in mice.", "content": "The teratogenic effects of methylmercuric chloride (MMC) given orally as a single dose to pregnant ICR mice on day 10 of gestation were examined. The doses tested were 25, 20, 15 and 10 mg/kg. Controls received distilled water orally. Each group consisted of 20 females. Fetuses were taken on day 18 of gestation for teratological study. The number of resorbed or dead embryos was moderately increased in the 25 mg/kg group. Fetuses from dams given 25, 20 and 15 mg/kg MMC weighed significantly less than those in the control group. Many fetuses with malformations were observed in the treated groups; cleft palate occurred in 100, 58.6 and 28.0% of fetuses from dams given 25, 20 and 15 mg/kg MMC, respectively (statistically significant). Hydronephrosis appeared in 23.8 and 18.5% of fetuses from dams given 25 and 20 mg/kg MMC, respectively (statistically significant). Skeletal variations, incomplete ossification of sternebrae, for example, were also observed in the treated groups. These results indicate that MMC is teratogenic so far as cleft palate is concerned and embryotoxic in ICR mice.", "contents": "Teratogenic effects of a single oral administration of methylmercuric chloride in mice. The teratogenic effects of methylmercuric chloride (MMC) given orally as a single dose to pregnant ICR mice on day 10 of gestation were examined. The doses tested were 25, 20, 15 and 10 mg/kg. Controls received distilled water orally. Each group consisted of 20 females. Fetuses were taken on day 18 of gestation for teratological study. The number of resorbed or dead embryos was moderately increased in the 25 mg/kg group. Fetuses from dams given 25, 20 and 15 mg/kg MMC weighed significantly less than those in the control group. Many fetuses with malformations were observed in the treated groups; cleft palate occurred in 100, 58.6 and 28.0% of fetuses from dams given 25, 20 and 15 mg/kg MMC, respectively (statistically significant). Hydronephrosis appeared in 23.8 and 18.5% of fetuses from dams given 25 and 20 mg/kg MMC, respectively (statistically significant). Skeletal variations, incomplete ossification of sternebrae, for example, were also observed in the treated groups. These results indicate that MMC is teratogenic so far as cleft palate is concerned and embryotoxic in ICR mice."}
{"id": "PMID:484204", "title": "\"Semi-open\" muscle biopsy technique. A simple outpatient procedure.", "content": "An easy and safe method for muscle biopsy is described. The biopsy instrument is an alligator forceps (Weil-Bladesley's conchotome). With this method an adequate amount of muscle tissue can be obtained for both chemical and morphological studies. The method combines the advantage of the \"open-surgical\" method - sufficient quantity of material - with the advantages of the needle methods - easy to perform, not incapacitating and only slightly uncomfortable for the patient. Out of 959 consecutive biopsies with this method only 19 were impossible to evaluate because of too much artefact or too little tissue.", "contents": "\"Semi-open\" muscle biopsy technique. A simple outpatient procedure. An easy and safe method for muscle biopsy is described. The biopsy instrument is an alligator forceps (Weil-Bladesley's conchotome). With this method an adequate amount of muscle tissue can be obtained for both chemical and morphological studies. The method combines the advantage of the \"open-surgical\" method - sufficient quantity of material - with the advantages of the needle methods - easy to perform, not incapacitating and only slightly uncomfortable for the patient. Out of 959 consecutive biopsies with this method only 19 were impossible to evaluate because of too much artefact or too little tissue."}
{"id": "PMID:484205", "title": "Visual evoked potential in patients with cerebral asthenopia.", "content": "Cerebral asthenopia is often overlooked as a symptom in diffuse brain lesion. An objective correlate of this symptom has so far never been demonstrated. Averaged visual evoked potential (VEP) in 10 patients with asthenopia was compared with 20 normal subjects. Both eyes and each eye alone were stimulated using bipolar recording in the midline and over each of the occipital lobes. There was no difference of latency of the VEPs in the two groups, but the amplitude of the most prominent component was significantly reduced in the patients. There was also a difference in the two groups regarding habituation and lateralisation. No amplitude difference could be found in the somatosensory evoked potential. The amplitude difference in VEP, as an objective correlate of asthenopia, is probably modal specific and suggests involvement of the visual cortex. VEP is unsuitable as a diagnostic tool due to the great overlap between amplitudes in asthenopic patients and control subjects.", "contents": "Visual evoked potential in patients with cerebral asthenopia. Cerebral asthenopia is often overlooked as a symptom in diffuse brain lesion. An objective correlate of this symptom has so far never been demonstrated. Averaged visual evoked potential (VEP) in 10 patients with asthenopia was compared with 20 normal subjects. Both eyes and each eye alone were stimulated using bipolar recording in the midline and over each of the occipital lobes. There was no difference of latency of the VEPs in the two groups, but the amplitude of the most prominent component was significantly reduced in the patients. There was also a difference in the two groups regarding habituation and lateralisation. No amplitude difference could be found in the somatosensory evoked potential. The amplitude difference in VEP, as an objective correlate of asthenopia, is probably modal specific and suggests involvement of the visual cortex. VEP is unsuitable as a diagnostic tool due to the great overlap between amplitudes in asthenopic patients and control subjects."}
{"id": "PMID:484201", "title": "[Early histogenesis in the cerebral cortex of the mouse: a Golgi analysis (author's transl)].", "content": "By the application of the rapid Golgi method to embryonic telencephalon, three stages can be distinguished in the early developmznt of the neocortex. Stage 1 is characterized by a neuroepithelium containing a continuously proliferating cell population. Stage 2 corresponds to the primordial cortical organization; the first neuronal (post-mitotic) cells appear in the external part of the neural tube and are differentiated in a predominantly horizontal pattern. Stage 3 begins when the cortical plate appears. This structure will give rise to the major part of the adult cortex. The cells of the cortical plate assume a typical radial orientation and their axons (afferent fibers) are well individualized. The afferent fibers however, are not easily analyzed by the technique employed. Those morphological findings are discussed in relation to various data from the literature in developmental neurology.", "contents": "[Early histogenesis in the cerebral cortex of the mouse: a Golgi analysis (author's transl)]. By the application of the rapid Golgi method to embryonic telencephalon, three stages can be distinguished in the early developmznt of the neocortex. Stage 1 is characterized by a neuroepithelium containing a continuously proliferating cell population. Stage 2 corresponds to the primordial cortical organization; the first neuronal (post-mitotic) cells appear in the external part of the neural tube and are differentiated in a predominantly horizontal pattern. Stage 3 begins when the cortical plate appears. This structure will give rise to the major part of the adult cortex. The cells of the cortical plate assume a typical radial orientation and their axons (afferent fibers) are well individualized. The afferent fibers however, are not easily analyzed by the technique employed. Those morphological findings are discussed in relation to various data from the literature in developmental neurology."}
{"id": "PMID:484206", "title": "Pigmentary degeneration of the retina in heredodegenerative neurological diseases.", "content": "Frequency of pigmentary degeneration of the retina (PDR) among patients with degenerative and heredodegenerative neurological diseases (HDNDs) was estimated based on the hospital statistics. PDR was detected in 3% of 176 inpatients with HDNDs by careful ophthalmologic examination. On the other hand, out of 30 consecutive cases of PDR seen in our Department of Neurology, 15 patients were associated with some form of HDNDs. Atypical PDR were more frequently associated with HDNDs than typical PDR. Among neurological manifestations in those 15 cases of PDR associated with HDNDs, mental deficiency, hearing disturbance, spasticity, progressive ophthalmoplegia and ataxia were most frequently encountered. Four cases with unusual symptomatology were presented. Clinical analysis of cases of PDR associated with HDNDs in the present series as well as in the relevant literature revealed an extreme variety of clinical manifestations and underlying metabolic disorders, suggesting a possible participation of multiple factors in the pathogenesis of PDR. Importance of careful ophthalmologic examination in HDNDs was stressed from the prognostic point of view.", "contents": "Pigmentary degeneration of the retina in heredodegenerative neurological diseases. Frequency of pigmentary degeneration of the retina (PDR) among patients with degenerative and heredodegenerative neurological diseases (HDNDs) was estimated based on the hospital statistics. PDR was detected in 3% of 176 inpatients with HDNDs by careful ophthalmologic examination. On the other hand, out of 30 consecutive cases of PDR seen in our Department of Neurology, 15 patients were associated with some form of HDNDs. Atypical PDR were more frequently associated with HDNDs than typical PDR. Among neurological manifestations in those 15 cases of PDR associated with HDNDs, mental deficiency, hearing disturbance, spasticity, progressive ophthalmoplegia and ataxia were most frequently encountered. Four cases with unusual symptomatology were presented. Clinical analysis of cases of PDR associated with HDNDs in the present series as well as in the relevant literature revealed an extreme variety of clinical manifestations and underlying metabolic disorders, suggesting a possible participation of multiple factors in the pathogenesis of PDR. Importance of careful ophthalmologic examination in HDNDs was stressed from the prognostic point of view."}
{"id": "PMID:484202", "title": "[Semiology of bismuth encephalopathy. Comparison with seven personal cases (author's transl)].", "content": "The authors analyze 99 well-described cases of bismuth encephalopathy and suggest a clinical syndrome according to three stages of the disease (before, during and after the acute period) and three clinical domains (psychiatry, neurology and neuropsychology). A particular attention concerns the presence of sequellar clinical signs (mnesic functions), sometimes observed one year after interruption of bismuth ingestion.", "contents": "[Semiology of bismuth encephalopathy. Comparison with seven personal cases (author's transl)]. The authors analyze 99 well-described cases of bismuth encephalopathy and suggest a clinical syndrome according to three stages of the disease (before, during and after the acute period) and three clinical domains (psychiatry, neurology and neuropsychology). A particular attention concerns the presence of sequellar clinical signs (mnesic functions), sometimes observed one year after interruption of bismuth ingestion."}
{"id": "PMID:484207", "title": "Fenestrated blood vessels in human skeletal muscle.", "content": "In a fine structural study of 32 skeletal muscle biopsies, rare examples of fenestrated blood vessels (FV) were found in three cases. In view of similar observations reported in a case of Duchenne muscular dystrophy, it is suggested that FV albeit rare may occur in skeletal muscle at least under pathologic conditions. These aberrant FV may be derived from adjacent dermis and/or epineurium which are known to contain occasional FV. Alternatively, the occurrence of fenestrae may reflect a nonspecific alteration in pre-existing continuous endothelium. The significance, if any, of their presence in skeletal muscle is unknown.", "contents": "Fenestrated blood vessels in human skeletal muscle. In a fine structural study of 32 skeletal muscle biopsies, rare examples of fenestrated blood vessels (FV) were found in three cases. In view of similar observations reported in a case of Duchenne muscular dystrophy, it is suggested that FV albeit rare may occur in skeletal muscle at least under pathologic conditions. These aberrant FV may be derived from adjacent dermis and/or epineurium which are known to contain occasional FV. Alternatively, the occurrence of fenestrae may reflect a nonspecific alteration in pre-existing continuous endothelium. The significance, if any, of their presence in skeletal muscle is unknown."}
{"id": "PMID:484208", "title": "Alterations in cat cerebrocortical capillary morphometrical parameters following K+-induced cerebrocortical swelling.", "content": "Histochemical, electron microscopic, and morphometrical techniques were employed in the determination of the effects attributed to K+-induced cerebrocortical swelling on cat cerebrocortical capillary diameter, length, surface area, volume, and minimal intercapillary distance. Bilaterally exposed and intact temporoparietal cerebral cortices of 4 conditioned adult cats were simultaneously superfused with isotonic, artificial CSF containing 3.5 mM K+ (control) and 54 mM K+ experimental), for 1h at 37 degrees C with monitoring of systemic vital function, hematocrit, arterial blood gases, and determination of cerebrocortical tissue water content. The mean values for cerebrocapillary diameter were 5% (P less than 0.05) greater in swollen tissues when compared with comparable mean values determined for controls. The values for minimal intercapillary distance determined from control and experimental animals plotted as relative frequency histograms represented two distinct populations (P less than 0.005). The significance of altered capillary morphometric parameters are discussed in relation to K+-induced cerebrocortical swelling.", "contents": "Alterations in cat cerebrocortical capillary morphometrical parameters following K+-induced cerebrocortical swelling. Histochemical, electron microscopic, and morphometrical techniques were employed in the determination of the effects attributed to K+-induced cerebrocortical swelling on cat cerebrocortical capillary diameter, length, surface area, volume, and minimal intercapillary distance. Bilaterally exposed and intact temporoparietal cerebral cortices of 4 conditioned adult cats were simultaneously superfused with isotonic, artificial CSF containing 3.5 mM K+ (control) and 54 mM K+ experimental), for 1h at 37 degrees C with monitoring of systemic vital function, hematocrit, arterial blood gases, and determination of cerebrocortical tissue water content. The mean values for cerebrocapillary diameter were 5% (P less than 0.05) greater in swollen tissues when compared with comparable mean values determined for controls. The values for minimal intercapillary distance determined from control and experimental animals plotted as relative frequency histograms represented two distinct populations (P less than 0.005). The significance of altered capillary morphometric parameters are discussed in relation to K+-induced cerebrocortical swelling."}
{"id": "PMID:484203", "title": "[Visuo-constructive disorders in right and left brain-damaged patients (author's transl)].", "content": "There seems to be some lack of agreement between clinical and experimental findings pertaining to the neurodynamic mechanisms of visuo-constructive disabilities in right and left brain-damaged patients. Clinical studies have revealed qualitative differences between drawing disabilities of right and left hemispheric origin, while experimental data suggest that the same mechanism underlies both types of constructional disorder. In order to reconcile these conflicting findings, the existence of \"nuclear\" impairment and more \"peripheral\" disturbances in constructional apraxia is suggested. Nuclear impairment seems to consist of a visuo-perceptive disorder independent of the hemispheric locus of lesion; peripheral disturbance could be different in right and left brain-damaged patients, motor and symbolic disorders being typical of left-sided lesions while unilateral spatial neglect would be characteristic of right hemispheric injuries.", "contents": "[Visuo-constructive disorders in right and left brain-damaged patients (author's transl)]. There seems to be some lack of agreement between clinical and experimental findings pertaining to the neurodynamic mechanisms of visuo-constructive disabilities in right and left brain-damaged patients. Clinical studies have revealed qualitative differences between drawing disabilities of right and left hemispheric origin, while experimental data suggest that the same mechanism underlies both types of constructional disorder. In order to reconcile these conflicting findings, the existence of \"nuclear\" impairment and more \"peripheral\" disturbances in constructional apraxia is suggested. Nuclear impairment seems to consist of a visuo-perceptive disorder independent of the hemispheric locus of lesion; peripheral disturbance could be different in right and left brain-damaged patients, motor and symbolic disorders being typical of left-sided lesions while unilateral spatial neglect would be characteristic of right hemispheric injuries."}
{"id": "PMID:484209", "title": "Normal and benzo(a)pyrene-transformed fetal mouse brain cell. I. Tumorigenicity and immunochemical detection of glial fibrillary acidic protein.", "content": "Primary cultures of whole brain and cortex cells origination from 14-day-old A/Jax or C3H mouse fetuses were treated with benzo(a)pyrene (B(a)P) for 24 h. After 7 to 8 passages a malignant transformation was observed in the chemically treated whole brain and cortex cultures. Control cultures of cortex remained non-transplantable during the whole experiment (up to 14 passages) whereas in the control cultures originating from whole brain a spontaneous transformation appeared after 11 passages. With horseradish peroxidase-labelled antibody, the specific glial fibrillary acidic protein (GFAP) was detected in both control and transformed total brain and cortex cultures, and in the tumors initiated by the in vitro transformed cells. This finding shows that glialike cells persisted after a long in vitro maintanance and transformation.", "contents": "Normal and benzo(a)pyrene-transformed fetal mouse brain cell. I. Tumorigenicity and immunochemical detection of glial fibrillary acidic protein. Primary cultures of whole brain and cortex cells origination from 14-day-old A/Jax or C3H mouse fetuses were treated with benzo(a)pyrene (B(a)P) for 24 h. After 7 to 8 passages a malignant transformation was observed in the chemically treated whole brain and cortex cultures. Control cultures of cortex remained non-transplantable during the whole experiment (up to 14 passages) whereas in the control cultures originating from whole brain a spontaneous transformation appeared after 11 passages. With horseradish peroxidase-labelled antibody, the specific glial fibrillary acidic protein (GFAP) was detected in both control and transformed total brain and cortex cultures, and in the tumors initiated by the in vitro transformed cells. This finding shows that glialike cells persisted after a long in vitro maintanance and transformation."}
{"id": "PMID:484210", "title": "Normal and benzo(a)pyrene-transformed fetal mouse brain cells. II. Ultrastructural study.", "content": "An ultrastructural study was performed on normal and Benzo(a)-pyrene(B(a)P)-transformed fetal mouse brain cells. Early subcultures of a strain initiated from whole brain presented three cell types in vitro: astroglial, poorly differentiated glial, and spongioblastic types. After B(a)P-treatment, there was an exclusive transformation and the growth of neuroglia sometimes without gliofibrillary maturation, but with the presence of glial fibrillary acidic protein (GFAP) in the cytoplasm. Early subcultures of another strain initiated from cortex only presented poorly differentiated neuroglial cells. After transformation, cell maturation as evidenced by gliofibrillogenesis and GFAP production by these cells was observed. In both cases, the potentiality of glial differentiation after in vitro malignant transformation by a chemical carcinogen seemed preserved.", "contents": "Normal and benzo(a)pyrene-transformed fetal mouse brain cells. II. Ultrastructural study. An ultrastructural study was performed on normal and Benzo(a)-pyrene(B(a)P)-transformed fetal mouse brain cells. Early subcultures of a strain initiated from whole brain presented three cell types in vitro: astroglial, poorly differentiated glial, and spongioblastic types. After B(a)P-treatment, there was an exclusive transformation and the growth of neuroglia sometimes without gliofibrillary maturation, but with the presence of glial fibrillary acidic protein (GFAP) in the cytoplasm. Early subcultures of another strain initiated from cortex only presented poorly differentiated neuroglial cells. After transformation, cell maturation as evidenced by gliofibrillogenesis and GFAP production by these cells was observed. In both cases, the potentiality of glial differentiation after in vitro malignant transformation by a chemical carcinogen seemed preserved."}
{"id": "PMID:484212", "title": "Amniotic fluid phosphatidylinositol and phosphatidylglycerol. I. Normal pregnancies.", "content": "Phosphatidylinositol (PI) and phosphatidylglycerol (PG) appear normally in the amniotic fluid during the last weeks of pregnancy. The present study indicated as have previous studies (5, 6, 8, 10) that these phospholipids are linked to the surfactant system in the fetal lung. The concentrations of lecithin (L), PI, PG and sphingomyelin (S) were measured in 207 samples from 165 normal pregnancies. The augmentation in PI was found to parallel that in lecithin, and the PI/S ratios reached maximum values at about 30 weeks of gestation. The augmentation in PG appeared about two weeks later. Eleven premature infants who contracted respiratory distress syndrome (RDS) had significantly lower PG concentrations than 15 premature infants with no RDS (p less than 0.01). No correlation to the PI concentrations could be observed. All but one of the 11 affected infants had low L/S ratios.", "contents": "Amniotic fluid phosphatidylinositol and phosphatidylglycerol. I. Normal pregnancies. Phosphatidylinositol (PI) and phosphatidylglycerol (PG) appear normally in the amniotic fluid during the last weeks of pregnancy. The present study indicated as have previous studies (5, 6, 8, 10) that these phospholipids are linked to the surfactant system in the fetal lung. The concentrations of lecithin (L), PI, PG and sphingomyelin (S) were measured in 207 samples from 165 normal pregnancies. The augmentation in PI was found to parallel that in lecithin, and the PI/S ratios reached maximum values at about 30 weeks of gestation. The augmentation in PG appeared about two weeks later. Eleven premature infants who contracted respiratory distress syndrome (RDS) had significantly lower PG concentrations than 15 premature infants with no RDS (p less than 0.01). No correlation to the PI concentrations could be observed. All but one of the 11 affected infants had low L/S ratios."}
{"id": "PMID:484213", "title": "Serum beta-human chorionic gonadotrophin levels in the early diagnosis of ectopic pregnancy.", "content": "Beta-HCG in serum was analysed in 64 cases of ectopic tubal pregnancy who wree different groups; ruptured ectopic pregnancy, ectopic pregnancy accompanied by amenorrhea or adnexal mass and ectopic pregnancy without palpable adnexal mass and amenorrhea. The mean HCG levels for the three groups were 8 790 IU/l, 2 580 IU/l and 690 IU/l, respectively, which related more to the symptoms than to the estimated length of pregnancy. Eleven per cent of the women had an IUD and five per cent were taking low dose gestagens. Screening of cases with acute lower abdominal pain or irregular vaginal bleeding with beta-HCG in serum will facilitate an early diagnosis of ectopic pregnancy and be of special value in patients with less typical symptoms.", "contents": "Serum beta-human chorionic gonadotrophin levels in the early diagnosis of ectopic pregnancy. Beta-HCG in serum was analysed in 64 cases of ectopic tubal pregnancy who wree different groups; ruptured ectopic pregnancy, ectopic pregnancy accompanied by amenorrhea or adnexal mass and ectopic pregnancy without palpable adnexal mass and amenorrhea. The mean HCG levels for the three groups were 8 790 IU/l, 2 580 IU/l and 690 IU/l, respectively, which related more to the symptoms than to the estimated length of pregnancy. Eleven per cent of the women had an IUD and five per cent were taking low dose gestagens. Screening of cases with acute lower abdominal pain or irregular vaginal bleeding with beta-HCG in serum will facilitate an early diagnosis of ectopic pregnancy and be of special value in patients with less typical symptoms."}
{"id": "PMID:484214", "title": "Effects of betamethasone on plasma levels of estriol, cortisol and HCS in late pregnancy.", "content": "The effect of a single dose of betamethasone on the maternal plasma concentration of estriol and cortisol was studied. The concentration of estriol decreased rapidly. A maximal suppression of about 70 per cent was seen after 6-24 hours. A similar influence on the maternal plasma concentration of cortisol was observed. HCS (human chorionic somatomammotropin) was not influenced by betamethasone. These facts have to be taken into consideration after treatment with synthetic corticosteroids in high risk pregnancies.", "contents": "Effects of betamethasone on plasma levels of estriol, cortisol and HCS in late pregnancy. The effect of a single dose of betamethasone on the maternal plasma concentration of estriol and cortisol was studied. The concentration of estriol decreased rapidly. A maximal suppression of about 70 per cent was seen after 6-24 hours. A similar influence on the maternal plasma concentration of cortisol was observed. HCS (human chorionic somatomammotropin) was not influenced by betamethasone. These facts have to be taken into consideration after treatment with synthetic corticosteroids in high risk pregnancies."}
{"id": "PMID:484215", "title": "Serum and amniotic fluid heat labile alkaline phosphatase and aminotransferases in association with meconium stained liquor. On their origin, distribution and clinical use.", "content": "A method for detecting meconium in the amniotic fluid is described. Samples of maternal serum, cord serum and amniotic fluid were tested for alkaline phosphatase and transaminases activity in 32 healthy, term gravidas during labor in whom meconium stained amniotic fluid was found. The values obtained were compared to those of a control group consisting of 32 normal term pregnancies in whom the amniotic fluid was clear. Mean alkaline phosphatase activity was significantly higher in the study group in all three compartments - maternal, fetal and amniotic fluid. A significant and positive correlation between levels of alkaline phosphatase in the amniotic fluid and in maternal serum was found in the study group. Alkaline phosphatase value of 550 IU/L or more in maternal serum was diagnostic and levels between 350--500 IU/L were highly suggestive for the presence of menconium in the amniotic fluid. Mean levels of transaminases (SGOT and SGPT) were similar in the two groups and all were within normal range. The origin of alkaline phosphatase and the mode of transfer of the three enzymes are also discussed. It is suggested that this method can also be used during the third trimester of pregnancy.", "contents": "Serum and amniotic fluid heat labile alkaline phosphatase and aminotransferases in association with meconium stained liquor. On their origin, distribution and clinical use. A method for detecting meconium in the amniotic fluid is described. Samples of maternal serum, cord serum and amniotic fluid were tested for alkaline phosphatase and transaminases activity in 32 healthy, term gravidas during labor in whom meconium stained amniotic fluid was found. The values obtained were compared to those of a control group consisting of 32 normal term pregnancies in whom the amniotic fluid was clear. Mean alkaline phosphatase activity was significantly higher in the study group in all three compartments - maternal, fetal and amniotic fluid. A significant and positive correlation between levels of alkaline phosphatase in the amniotic fluid and in maternal serum was found in the study group. Alkaline phosphatase value of 550 IU/L or more in maternal serum was diagnostic and levels between 350--500 IU/L were highly suggestive for the presence of menconium in the amniotic fluid. Mean levels of transaminases (SGOT and SGPT) were similar in the two groups and all were within normal range. The origin of alkaline phosphatase and the mode of transfer of the three enzymes are also discussed. It is suggested that this method can also be used during the third trimester of pregnancy."}
{"id": "PMID:484216", "title": "Effect of induction of general anesthesia for cesarean section on intervillous blood flow.", "content": "Intervillous blood flow was measured by a new intravenous 133Xe method before and during induction of general anesthesia for cesarean section in 10 healthy mothers. The flow values showed a highly significant decrease (p less than 0.001) (35 per cent on an average) at the time of anesthesia compared with the control values. The impairment was observed in all the cases. The role of the maternal changes in hemodynamic parameters and acid-base balance as a background of this decrease is discussed.", "contents": "Effect of induction of general anesthesia for cesarean section on intervillous blood flow. Intervillous blood flow was measured by a new intravenous 133Xe method before and during induction of general anesthesia for cesarean section in 10 healthy mothers. The flow values showed a highly significant decrease (p less than 0.001) (35 per cent on an average) at the time of anesthesia compared with the control values. The impairment was observed in all the cases. The role of the maternal changes in hemodynamic parameters and acid-base balance as a background of this decrease is discussed."}
{"id": "PMID:484217", "title": "Pregnancy complications following conization of the uterine cervix (I).", "content": "The literature on the course of pregnancies following conization of the uterine cervix has been studied. It is demonstrated that an evaluation of the possible effect of conization upon subsequent pregnancy should be based upon a comparison of either pregnancies before and after conization or upon a comparison between pregnancies in women with a previous conization and in a control group of women without conization. In both cases the possible influence of conization can be evaluated only if the patient material is described as regards age, parity, number of previous pregnancies, smoking habits, etc., factors which may all influence the course of pregnancy. None of the previous publications have described the patient material sufficiently, and most studies have not tried to set up control groups. Due to these deficiencies we do not find it justified that conization leads to reduced fertility, increased frequency of spontaneous abortion, nor to increased perinatal mortality. An increased prematurity rate may not be rejected, however, but this point has not been adequately evaluated in the previous papers.", "contents": "Pregnancy complications following conization of the uterine cervix (I). The literature on the course of pregnancies following conization of the uterine cervix has been studied. It is demonstrated that an evaluation of the possible effect of conization upon subsequent pregnancy should be based upon a comparison of either pregnancies before and after conization or upon a comparison between pregnancies in women with a previous conization and in a control group of women without conization. In both cases the possible influence of conization can be evaluated only if the patient material is described as regards age, parity, number of previous pregnancies, smoking habits, etc., factors which may all influence the course of pregnancy. None of the previous publications have described the patient material sufficiently, and most studies have not tried to set up control groups. Due to these deficiencies we do not find it justified that conization leads to reduced fertility, increased frequency of spontaneous abortion, nor to increased perinatal mortality. An increased prematurity rate may not be rejected, however, but this point has not been adequately evaluated in the previous papers."}
{"id": "PMID:484218", "title": "Evaluation of smears obtained by cervical scraping and an endocervical swab in the diagnosis of neoplastic disease of the uterine cervix.", "content": "A study of the supplementary value of an endocervical swab smear in addition to cervical scraping in the cytological diagnosis of cervical neoplasias is presented. The two sampling techniques were applied to a population with a high prevalence of neoplastic cervical disease. The endocervical swab smear was a useful adjunct in the detection of mild and moderate dysplasia, and a combination of the two sampling methods decreased the false negative rate in the diagnosis of intraepithelial, as well as invasive neoplasia. In the sphere of specific cytological diagnosis cervical scraping was found to be the more accurate method for diagnosing severe dysplasia and carcinoma in situ, while endocervical swab smears were more useful in diagnosing mild and moderate dysplasia. Differences between the results obtained in our study and comparable studies are discussed. It is concluded that the endocervical swab smear is a valuable adjunct to cervical scraping in the diagnosis of malignant cervical disease. It should not, however, be used as the only sampling method as it produces a higher proportion of unsatisfactory smears, and also because the severity of the epithelial lesion is more likely to be underestimated.", "contents": "Evaluation of smears obtained by cervical scraping and an endocervical swab in the diagnosis of neoplastic disease of the uterine cervix. A study of the supplementary value of an endocervical swab smear in addition to cervical scraping in the cytological diagnosis of cervical neoplasias is presented. The two sampling techniques were applied to a population with a high prevalence of neoplastic cervical disease. The endocervical swab smear was a useful adjunct in the detection of mild and moderate dysplasia, and a combination of the two sampling methods decreased the false negative rate in the diagnosis of intraepithelial, as well as invasive neoplasia. In the sphere of specific cytological diagnosis cervical scraping was found to be the more accurate method for diagnosing severe dysplasia and carcinoma in situ, while endocervical swab smears were more useful in diagnosing mild and moderate dysplasia. Differences between the results obtained in our study and comparable studies are discussed. It is concluded that the endocervical swab smear is a valuable adjunct to cervical scraping in the diagnosis of malignant cervical disease. It should not, however, be used as the only sampling method as it produces a higher proportion of unsatisfactory smears, and also because the severity of the epithelial lesion is more likely to be underestimated."}
{"id": "PMID:484219", "title": "The cryosurgical treatment of intraepithelial neoplasia.", "content": "At the Finseninstituttet and the Radium Centre in Copenhagen, from 1971-1975 inclusive, cryosurgery was carried out altogether 59 times on 57 patients. Only 8 patients showed histopathological changes in the biopsies sufficient to indicate conization. Hysterectomy was carried out on 8 patients. In 7 of these the removed uteri showed either no malignant changes or changpy. Invasive growth was discovered only in 1 patient, who later underwent a total hysterectomy by the Wertheim method. A biopsy on 1 patient with carcinoma in situ, suspected invasive growth, was treated with radium.", "contents": "The cryosurgical treatment of intraepithelial neoplasia. At the Finseninstituttet and the Radium Centre in Copenhagen, from 1971-1975 inclusive, cryosurgery was carried out altogether 59 times on 57 patients. Only 8 patients showed histopathological changes in the biopsies sufficient to indicate conization. Hysterectomy was carried out on 8 patients. In 7 of these the removed uteri showed either no malignant changes or changpy. Invasive growth was discovered only in 1 patient, who later underwent a total hysterectomy by the Wertheim method. A biopsy on 1 patient with carcinoma in situ, suspected invasive growth, was treated with radium."}
{"id": "PMID:484220", "title": "Methods for evaluating the intrauterine location of carcinoma.", "content": "The intrauterine location of endometrial carcinoma was investigated with curettage, hysterography and hysteroscopy in 83 patients. These three methods are complementary. Curettage provides important information about the differentiation of the tumor. Hysterography is suitable especially for elucidating the anatomy of the cervical canal and uterine cavity. Direct examination by hysteroscopy gives a reliable picture when defining the boundary between stages I and II.", "contents": "Methods for evaluating the intrauterine location of carcinoma. The intrauterine location of endometrial carcinoma was investigated with curettage, hysterography and hysteroscopy in 83 patients. These three methods are complementary. Curettage provides important information about the differentiation of the tumor. Hysterography is suitable especially for elucidating the anatomy of the cervical canal and uterine cavity. Direct examination by hysteroscopy gives a reliable picture when defining the boundary between stages I and II."}
{"id": "PMID:484221", "title": "Menorrhagia, diffuse myometrial hypertrophy and the intrauterine contraceptive device: a report of fourteen cases.", "content": "Fourteen uteri, removed for IUD-associated menorrhagia, were studied. Twelve of these IUD-bearing uteri showed pure diffuse myometrial hypertrophy; the other two uteri were enlarged as a result of multiple leiomyomas in one case and extensive deep adenomyosis in the other. The incidence of pure myometrial hypertrophy in the IUD group was far in excess of that observed in a control non-IUD-bearing menorrhagia series, where leiomyomas and/or deep adenomyosis were mostly responsible for the uterine enlargement.", "contents": "Menorrhagia, diffuse myometrial hypertrophy and the intrauterine contraceptive device: a report of fourteen cases. Fourteen uteri, removed for IUD-associated menorrhagia, were studied. Twelve of these IUD-bearing uteri showed pure diffuse myometrial hypertrophy; the other two uteri were enlarged as a result of multiple leiomyomas in one case and extensive deep adenomyosis in the other. The incidence of pure myometrial hypertrophy in the IUD group was far in excess of that observed in a control non-IUD-bearing menorrhagia series, where leiomyomas and/or deep adenomyosis were mostly responsible for the uterine enlargement."}
{"id": "PMID:484222", "title": "Biological fate of methenamine in man. Absorption, renal excretion and passage to umbilical cord blood, amniotic fluid and breast milk.", "content": "Methenamine hippurate was administered orally as tablets or granules to healthy volunteers. Plasma concentrations of methenamine reached a maximum 1--2 hours after a single dose and then declined with a half-life of about 4 hours. The apparent distribution volume was similar to that of total body water. Renal clearance of methenamine was somewhat lower than that of creatinine. In cross-over experiments over six days, methenamine recovered in the urine corresponded to about 80 per cent of the dose given per 12 hours, slightly lower values being obtained from granules than from tablets. The efficient renal elimination of methenamine was confirmed in similar studies on patients post-operatively. Methenamine hippurate was also given to healthy pregnant women during labor, a few hours before expected delivery. Methenamine was found to pass the placental barrier. The concentration of methenamine in umbilical cord plasma was low but reached the level in maternal plasma after about 4 hours. In amniotic fluid the methenamine con centration was low and varying. No correlation was obtained to the maternal or umbilical cord plasma levels. The methenamine concentration in breast milk was of the same magnitude as in maternal plasma. It is concluded that methenamine may be safely given to pregnant and lactating women with respect to the ellbeing of the child.", "contents": "Biological fate of methenamine in man. Absorption, renal excretion and passage to umbilical cord blood, amniotic fluid and breast milk. Methenamine hippurate was administered orally as tablets or granules to healthy volunteers. Plasma concentrations of methenamine reached a maximum 1--2 hours after a single dose and then declined with a half-life of about 4 hours. The apparent distribution volume was similar to that of total body water. Renal clearance of methenamine was somewhat lower than that of creatinine. In cross-over experiments over six days, methenamine recovered in the urine corresponded to about 80 per cent of the dose given per 12 hours, slightly lower values being obtained from granules than from tablets. The efficient renal elimination of methenamine was confirmed in similar studies on patients post-operatively. Methenamine hippurate was also given to healthy pregnant women during labor, a few hours before expected delivery. Methenamine was found to pass the placental barrier. The concentration of methenamine in umbilical cord plasma was low but reached the level in maternal plasma after about 4 hours. In amniotic fluid the methenamine con centration was low and varying. No correlation was obtained to the maternal or umbilical cord plasma levels. The methenamine concentration in breast milk was of the same magnitude as in maternal plasma. It is concluded that methenamine may be safely given to pregnant and lactating women with respect to the ellbeing of the child."}
{"id": "PMID:484223", "title": "Minimal brain dysfunction in children born in breech presentation.", "content": "The influence of delivery in breech presentation on the occurrence of minimal brain dysfunction (MBD) has been studied. Parents of 8--15 year old children were asked to account for behavioral and educational deficits and needs of their children from birth until the date of investigation. It was found that among prematurely delivered children, the frequency of MBD, defined as hyperkinesia and learning disability, amounted to about 20 per cent and was similarly distributed between children , however, the average frequency of hyperkinesia and learning disability amounted to 8 per cent; in children born in breech presentation it was 14 per cent, while in those born in vertex presentation the corresponding figure was 2 per cent. MBD was more common in boys (16 per cent) than in girls (12 per cent).", "contents": "Minimal brain dysfunction in children born in breech presentation. The influence of delivery in breech presentation on the occurrence of minimal brain dysfunction (MBD) has been studied. Parents of 8--15 year old children were asked to account for behavioral and educational deficits and needs of their children from birth until the date of investigation. It was found that among prematurely delivered children, the frequency of MBD, defined as hyperkinesia and learning disability, amounted to about 20 per cent and was similarly distributed between children , however, the average frequency of hyperkinesia and learning disability amounted to 8 per cent; in children born in breech presentation it was 14 per cent, while in those born in vertex presentation the corresponding figure was 2 per cent. MBD was more common in boys (16 per cent) than in girls (12 per cent)."}
{"id": "PMID:484224", "title": "Infertility and cervical Chlamydia trachomatis infections.", "content": "Of the 51 women examined for infertility, 19.6 per cent were found Chl. trachomatis culture-positive. This differs, although not significantly, from the 9 per cent isolation rate among our general gynecological outpatients. The results suggest that Chl. trachomatis should be considered in women with unexplained infertility.", "contents": "Infertility and cervical Chlamydia trachomatis infections. Of the 51 women examined for infertility, 19.6 per cent were found Chl. trachomatis culture-positive. This differs, although not significantly, from the 9 per cent isolation rate among our general gynecological outpatients. The results suggest that Chl. trachomatis should be considered in women with unexplained infertility."}
{"id": "PMID:484225", "title": "X/X translocation and Turner's syndrome in a woman with climacterium praecox.", "content": "A woman with X/X translocation is presented, and the association between the different types of X/X translocation and Turner's syndrome as well as the question of menstruations and fertility in women with Turner's syndrome are discussed. It is concluded that streak gonads should most probably not be included in the definition of Turner's syndrome.", "contents": "X/X translocation and Turner's syndrome in a woman with climacterium praecox. A woman with X/X translocation is presented, and the association between the different types of X/X translocation and Turner's syndrome as well as the question of menstruations and fertility in women with Turner's syndrome are discussed. It is concluded that streak gonads should most probably not be included in the definition of Turner's syndrome."}
{"id": "PMID:484226", "title": "Maternal sepsis, uterine rupture and coagulopathy complicating cervical cerclage.", "content": "A previously healthy woman with a Shirodkar cerclage for cervical incompetence had a spontaneous rupture of the membranes at the 37th week of pregnancy. Three days later after a short period of weak labor pains, she developed a severe sepsis, uterine rupture and coagulopathy leading to renal failure, beta-hemolytic streptococcus group B and peptostreptococcous could be cultured from the amniotic fluid immediately after rupture of the membranes and from the uterus and placenta.", "contents": "Maternal sepsis, uterine rupture and coagulopathy complicating cervical cerclage. A previously healthy woman with a Shirodkar cerclage for cervical incompetence had a spontaneous rupture of the membranes at the 37th week of pregnancy. Three days later after a short period of weak labor pains, she developed a severe sepsis, uterine rupture and coagulopathy leading to renal failure, beta-hemolytic streptococcus group B and peptostreptococcous could be cultured from the amniotic fluid immediately after rupture of the membranes and from the uterus and placenta."}
{"id": "PMID:484229", "title": "Vestibular neuronitis; a follow-up study.", "content": "Forty-three patients suffering from vestibular neuronitis were examinated during the acute attack and on an average 3.2 years afterwards. In the follow-up examination there was no caloric reactions in 7 patients, lowered in 15 patients and normal symmetric reactions in 21 persons. In the first examination all had normal symmetric hearing, in the follow-up examination there was 5 to 10 db hearing loss in 4.6 and 8 kHz on the diseased side in 24 patients.", "contents": "Vestibular neuronitis; a follow-up study. Forty-three patients suffering from vestibular neuronitis were examinated during the acute attack and on an average 3.2 years afterwards. In the follow-up examination there was no caloric reactions in 7 patients, lowered in 15 patients and normal symmetric reactions in 21 persons. In the first examination all had normal symmetric hearing, in the follow-up examination there was 5 to 10 db hearing loss in 4.6 and 8 kHz on the diseased side in 24 patients."}
{"id": "PMID:484234", "title": "On pathogenesis of symptoms in M\u00e9ni\u00e8re's disease.", "content": "A conception of the mechanism for initiating symptoms in M\u00e9ni\u00e8re's disease has been forwarded. According to this conception production of endolymph may vary also in normals. The hydrops in M\u00e9ni\u00e8re cases is brought about by a relative insufficiency in endolymphatic draining routes and the hydrops presents itself only at peak endolymph production periods. The attacks of vertigo are brought about at the distension of the sacs as well as of the regaining of normal shape of the sacs. The mechanism causing the vertigo could be an effect of mechanical push-and-pull effect on the sensory epithelium of the cristae. The early low-tone hearing loss and the persistant normal caloric reactivity also in advanced M\u00e9i\u00e8re cases may be due to simple physical laws : distensions of sacs with equal walls will more easily be brought about in wider than narrow compartments.", "contents": "On pathogenesis of symptoms in M\u00e9ni\u00e8re's disease. A conception of the mechanism for initiating symptoms in M\u00e9ni\u00e8re's disease has been forwarded. According to this conception production of endolymph may vary also in normals. The hydrops in M\u00e9ni\u00e8re cases is brought about by a relative insufficiency in endolymphatic draining routes and the hydrops presents itself only at peak endolymph production periods. The attacks of vertigo are brought about at the distension of the sacs as well as of the regaining of normal shape of the sacs. The mechanism causing the vertigo could be an effect of mechanical push-and-pull effect on the sensory epithelium of the cristae. The early low-tone hearing loss and the persistant normal caloric reactivity also in advanced M\u00e9i\u00e8re cases may be due to simple physical laws : distensions of sacs with equal walls will more easily be brought about in wider than narrow compartments."}
{"id": "PMID:484237", "title": "Activation of human monocytes by mediators from lymphocytes stimulated with Corynebacterium parvum.", "content": "Human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by Corynebacterium parvum (CP) expressed increased ability to suppress DNA-synthesis in a human tumour cell line. Monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. The lymphokine-induced cytostatic ability was not affected by intensive washing and trypsin treatment of the activated monocytes, but disappeared during 48 hours of in vitro culture of the activated cells. The increased cytostatic ability of lymphokine-activated monocytes did not seem to be due to stable supernatant factors released from monocytes. CP stimulated DNA-synthesis in peripheral blood lymphocytes of 28 normal donors, thus confirming the mitogenic effect of CP on human lymphocytes. Lymphokine production in response to CP correlated with the magnitude of DNA-synthesis, but appeared before DNA-synthesis could be detected in the lymphocytes.", "contents": "Activation of human monocytes by mediators from lymphocytes stimulated with Corynebacterium parvum. Human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by Corynebacterium parvum (CP) expressed increased ability to suppress DNA-synthesis in a human tumour cell line. Monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. The lymphokine-induced cytostatic ability was not affected by intensive washing and trypsin treatment of the activated monocytes, but disappeared during 48 hours of in vitro culture of the activated cells. The increased cytostatic ability of lymphokine-activated monocytes did not seem to be due to stable supernatant factors released from monocytes. CP stimulated DNA-synthesis in peripheral blood lymphocytes of 28 normal donors, thus confirming the mitogenic effect of CP on human lymphocytes. Lymphokine production in response to CP correlated with the magnitude of DNA-synthesis, but appeared before DNA-synthesis could be detected in the lymphocytes."}
{"id": "PMID:484238", "title": "The binding of protein A of immunoglobulin G and of Fab and Fc fragments.", "content": "Results are presented to show that protein A (pA) may fix to the Fab region of IgG outside the antigen binding site. Thus, pA-reactive Fab fragments were isolated both from specific anti-egg albumin and specific anti-measles virus antibodies. A probable significance of this Fab reactivity in the precipitation reaction between IgG and pA is discussed. Reactive IgG from human, guinea pig, rabbit, and porcine normal sera all showed similar avidity towards pA. Slight differences between IgG subclasses were, however, observed. The avidity of pA-reactive Fab and Fc fragments, isolated from normal porcine IgG, was highest in the latter.", "contents": "The binding of protein A of immunoglobulin G and of Fab and Fc fragments. Results are presented to show that protein A (pA) may fix to the Fab region of IgG outside the antigen binding site. Thus, pA-reactive Fab fragments were isolated both from specific anti-egg albumin and specific anti-measles virus antibodies. A probable significance of this Fab reactivity in the precipitation reaction between IgG and pA is discussed. Reactive IgG from human, guinea pig, rabbit, and porcine normal sera all showed similar avidity towards pA. Slight differences between IgG subclasses were, however, observed. The avidity of pA-reactive Fab and Fc fragments, isolated from normal porcine IgG, was highest in the latter."}
{"id": "PMID:484251", "title": "The effects of a beta 1-blocking agent, atenolol, on blood pressure, plasma renin activity and prostaglandin F2 alpha excretion in patients with essential hypertension.", "content": "The antihypertensive action of beta-blocking agents has been suggested to be associated with the decrease in plasma renin activity (PRA) and can be antagonized by indomethacin, a prostaglandin (PG) synthesis inhibitor. We studied the acute and long-term effects of a beta 1-blocking agent, atenolol (50 mg b.i.d.), on blood pressure (BP), PRA and urinary PGF2 alpha excretion in 12 male patients (40 years old) with essential hypertension. BP was measured by means of a brachial cuff. PRA and PGF2 alpha were estimated radioimmunologically. One day after the initiation of atenolol treatment, BP fell significantly, the supine values from 159/114 to 143/104 mmHg and the erect from 158/118 to 140/106 mmHg. In six weeks BP decreased further to 135/94 and 134/96 mmHg, respectively. After the cessation of atenolol for three weeks BP rose to the pre-atenolol level. When the dose was readjusted (25-150 mg daily for 26 weeks), diastolic BP remained at 100 mmHg or higher in only two patients. During the atenolol treatment PRA declined to one-third of the pre-atenolol level in one day and to one-half in six weeks. The urinary excretion of PGF2 alpha was not affected by atenolol. Our results suggest that 1) the antihypertensive action of atenolol and the reduction of PRA are substantial already in one day, and 2) the decrease in BP or PRA is not associated with PGF2 alpha production.", "contents": "The effects of a beta 1-blocking agent, atenolol, on blood pressure, plasma renin activity and prostaglandin F2 alpha excretion in patients with essential hypertension. The antihypertensive action of beta-blocking agents has been suggested to be associated with the decrease in plasma renin activity (PRA) and can be antagonized by indomethacin, a prostaglandin (PG) synthesis inhibitor. We studied the acute and long-term effects of a beta 1-blocking agent, atenolol (50 mg b.i.d.), on blood pressure (BP), PRA and urinary PGF2 alpha excretion in 12 male patients (40 years old) with essential hypertension. BP was measured by means of a brachial cuff. PRA and PGF2 alpha were estimated radioimmunologically. One day after the initiation of atenolol treatment, BP fell significantly, the supine values from 159/114 to 143/104 mmHg and the erect from 158/118 to 140/106 mmHg. In six weeks BP decreased further to 135/94 and 134/96 mmHg, respectively. After the cessation of atenolol for three weeks BP rose to the pre-atenolol level. When the dose was readjusted (25-150 mg daily for 26 weeks), diastolic BP remained at 100 mmHg or higher in only two patients. During the atenolol treatment PRA declined to one-third of the pre-atenolol level in one day and to one-half in six weeks. The urinary excretion of PGF2 alpha was not affected by atenolol. Our results suggest that 1) the antihypertensive action of atenolol and the reduction of PRA are substantial already in one day, and 2) the decrease in BP or PRA is not associated with PGF2 alpha production."}
{"id": "PMID:484252", "title": "The diagnostic challenge of left atrial myxoma. Importance of echocardiographic screening.", "content": "Left atrial myxomas are extremely difficult to diagnose since their variable manifestations mimic a host of clinical entities more commonly seen, e.g. mitral stenosis, endocarditis, rheumatic fever, cardiomyopathy or mesenchymosis. At the same time, early diagnosis followed by prompt surgical removal are mandatory to prevent mutilating or lethal complications of the tumor. Six cases of left atrial myxoma were diagnosed in our hospital during 2 1/2 years. We present the case histories, diagnostic procedures and surgical findings, consolidating the unique role of echocardiography in detecting left atrial myxomas. We propose the use of echocardiography as a screening examination for atrial myxomas in the following settings: suspected mitral obstructive disease, suspected endocarditis with negative blood cultures, peripheral embolism or thrombosis in young patients, unexplained cardiac failure and mesenchymosis with uncharacteristic presentation.", "contents": "The diagnostic challenge of left atrial myxoma. Importance of echocardiographic screening. Left atrial myxomas are extremely difficult to diagnose since their variable manifestations mimic a host of clinical entities more commonly seen, e.g. mitral stenosis, endocarditis, rheumatic fever, cardiomyopathy or mesenchymosis. At the same time, early diagnosis followed by prompt surgical removal are mandatory to prevent mutilating or lethal complications of the tumor. Six cases of left atrial myxoma were diagnosed in our hospital during 2 1/2 years. We present the case histories, diagnostic procedures and surgical findings, consolidating the unique role of echocardiography in detecting left atrial myxomas. We propose the use of echocardiography as a screening examination for atrial myxomas in the following settings: suspected mitral obstructive disease, suspected endocarditis with negative blood cultures, peripheral embolism or thrombosis in young patients, unexplained cardiac failure and mesenchymosis with uncharacteristic presentation."}
{"id": "PMID:484253", "title": "Massive embolization of cardiac myxoma. A case report.", "content": "A case of left atrial myxoma with massive embolization in a 20-year-old woman is described. The initial clinical picture was confusing, but repeated examinations, including enzyme determinations and arteriographies, disclosed the presence of multiple arterial emboli. At embolectomy, histological investigation of embolic masses showed a picture indicating cardiac myxoma. This diagnosis was confirmed by pulmonary angiography and the tumour was removed. Postischaemic oedema of the legs necessitated decompression operations. The peripheral circulation was restored and the final result was excellent. Some aspects of the clinical presentation of this uncommon disease are discussed.", "contents": "Massive embolization of cardiac myxoma. A case report. A case of left atrial myxoma with massive embolization in a 20-year-old woman is described. The initial clinical picture was confusing, but repeated examinations, including enzyme determinations and arteriographies, disclosed the presence of multiple arterial emboli. At embolectomy, histological investigation of embolic masses showed a picture indicating cardiac myxoma. This diagnosis was confirmed by pulmonary angiography and the tumour was removed. Postischaemic oedema of the legs necessitated decompression operations. The peripheral circulation was restored and the final result was excellent. Some aspects of the clinical presentation of this uncommon disease are discussed."}
{"id": "PMID:484254", "title": "Diastolic wave initiating ventricular tachyarrhythmias and suppressible by lignocaine and isoprenaline.", "content": "A patient is described with ventricular tachycardia and premature beats (VPB) probably initiated by a diastolic wave, which--like the VPBs--could be eliminated by lignocaine and isoprenaline.", "contents": "Diastolic wave initiating ventricular tachyarrhythmias and suppressible by lignocaine and isoprenaline. A patient is described with ventricular tachycardia and premature beats (VPB) probably initiated by a diastolic wave, which--like the VPBs--could be eliminated by lignocaine and isoprenaline."}
{"id": "PMID:484255", "title": "Effects on muscle electrolytes of potassium and magnesium infusions, spironolactone medication and operation in a case of primary aldosteronism.", "content": "Serum and muscle electrolytes were determined in a case of primary aldosteronism before and after potassium and magnesium infusions as well as spironolactone treatment and following surgery. Repeated potassium infusions resulted in a transient normalization of the muscle potassium (K/m), followed within 3-4 days by a return to the previously low K/m. Magnesium infusions did not give any increase in muscle magnesium (Mg/m). On the contrary, there was a decrease in Mg/m concomitant with a decrease in K/m. Treatment with spironolactone for one month was followed by a normalization of both serum and muscle electrolytes. Following surgery the serum potassium and K/m remained normal, but the serum magnesium (Mg/s) and Mg/m showed a decrease to subnormal values. Despite the initial findings of normal Mg/s and Mg/m as well as excretion of more than 80% of the i.v. magnesium dose, this may indicate that there was a magnesium deficiency in the skeletal pool.", "contents": "Effects on muscle electrolytes of potassium and magnesium infusions, spironolactone medication and operation in a case of primary aldosteronism. Serum and muscle electrolytes were determined in a case of primary aldosteronism before and after potassium and magnesium infusions as well as spironolactone treatment and following surgery. Repeated potassium infusions resulted in a transient normalization of the muscle potassium (K/m), followed within 3-4 days by a return to the previously low K/m. Magnesium infusions did not give any increase in muscle magnesium (Mg/m). On the contrary, there was a decrease in Mg/m concomitant with a decrease in K/m. Treatment with spironolactone for one month was followed by a normalization of both serum and muscle electrolytes. Following surgery the serum potassium and K/m remained normal, but the serum magnesium (Mg/s) and Mg/m showed a decrease to subnormal values. Despite the initial findings of normal Mg/s and Mg/m as well as excretion of more than 80% of the i.v. magnesium dose, this may indicate that there was a magnesium deficiency in the skeletal pool."}
{"id": "PMID:484256", "title": "Non-selective and selective beta-1-adrenoceptor blocking agents in the treatment of hyperthyroidism.", "content": "Treatment for one month with propranolol or atenolol, a selective beta-1-adrenoceptor blocking agent, was evaluated in 20 hyperthyroid patients. The patients improved to the same extent on either drug, as shown by a clinical diagnostic index. Basal metabolic rate decreased by 11% during both treatments, while it was unchanged in seven untreated hyperthyroid controls. Thyroxine concentration did not change during any treatment. During propranolol treatment T3 decreased from 4.6 to 3.9 nmol/l, while no changes were observed during atenolol treatment or in the control group. No significant changes were seen in free T4, free T3 or rT3 concentrations on any treatment, although free T3 was observed to decrease slightly during propranolol treatment. Thus, the improvement of the clinical symptoms of hyperthyroidism cannot be explained by diminished thyroid hormone concentrations in serum, since the reduction was small during propranolol and absent during atenolol treatment.", "contents": "Non-selective and selective beta-1-adrenoceptor blocking agents in the treatment of hyperthyroidism. Treatment for one month with propranolol or atenolol, a selective beta-1-adrenoceptor blocking agent, was evaluated in 20 hyperthyroid patients. The patients improved to the same extent on either drug, as shown by a clinical diagnostic index. Basal metabolic rate decreased by 11% during both treatments, while it was unchanged in seven untreated hyperthyroid controls. Thyroxine concentration did not change during any treatment. During propranolol treatment T3 decreased from 4.6 to 3.9 nmol/l, while no changes were observed during atenolol treatment or in the control group. No significant changes were seen in free T4, free T3 or rT3 concentrations on any treatment, although free T3 was observed to decrease slightly during propranolol treatment. Thus, the improvement of the clinical symptoms of hyperthyroidism cannot be explained by diminished thyroid hormone concentrations in serum, since the reduction was small during propranolol and absent during atenolol treatment."}
{"id": "PMID:484257", "title": "Self-poisoning treated in the ICU.", "content": "This retrospective study compares number and type of self-poisonings admitted to the intensive care units (ICU) at Sahlgren's Hospital, Gothenburg, during 1972 and 1976. The total number of patients requiring intensive care was unchanged, but a change in the pattern of self-poisoning was seen. In 1976 the number of patients requiring intensive care following acute alcohol intoxication had increased, whereas the number of barbiturate and methaqualone poisonings decreased. Tricyclic antidepressants formed the largest group in both years. Fewer patients needed endotracheal intubation and IPPV in 1976 than in 1972. Also, fewer complications were seen, and the death rate among ICU-treated patients decreased. In the Gothenburg area barbiturates accounted for one half of all deaths due to self-poisoning and tricyclic antidepressants for one fifth. Any expected decrease in intensive care as a result of fewer barbiturate and methaqualone poisonings was obscured by an increase in severe alcohol intoxication.", "contents": "Self-poisoning treated in the ICU. This retrospective study compares number and type of self-poisonings admitted to the intensive care units (ICU) at Sahlgren's Hospital, Gothenburg, during 1972 and 1976. The total number of patients requiring intensive care was unchanged, but a change in the pattern of self-poisoning was seen. In 1976 the number of patients requiring intensive care following acute alcohol intoxication had increased, whereas the number of barbiturate and methaqualone poisonings decreased. Tricyclic antidepressants formed the largest group in both years. Fewer patients needed endotracheal intubation and IPPV in 1976 than in 1972. Also, fewer complications were seen, and the death rate among ICU-treated patients decreased. In the Gothenburg area barbiturates accounted for one half of all deaths due to self-poisoning and tricyclic antidepressants for one fifth. Any expected decrease in intensive care as a result of fewer barbiturate and methaqualone poisonings was obscured by an increase in severe alcohol intoxication."}
{"id": "PMID:484258", "title": "Emergency room resuscitation of patients with cardiac arrest outside hospital. Outcome and immediate prognosis in 319 patients.", "content": "Resuscitation was attempted in 319 patients brought to hospital with cardiac arrest during a 5-year period. Primary successful results were achieved in 50 patients (15.7%). Twelve patients were long-term survivors (3.4%), 10 of whom had normal brain function, whereas 2 had mild cerebral dysfunction. To improve prognostication in patients with initially successful resuscitation, Bayes' theorem was applied using 4 clinical findings after 24 hours' treatment: reactions to painful stimuli, pupillary size, light reactions and BP, Bayes' theorem as well as coma depth after 24 hours gave valuable information regarding individual prognosis.", "contents": "Emergency room resuscitation of patients with cardiac arrest outside hospital. Outcome and immediate prognosis in 319 patients. Resuscitation was attempted in 319 patients brought to hospital with cardiac arrest during a 5-year period. Primary successful results were achieved in 50 patients (15.7%). Twelve patients were long-term survivors (3.4%), 10 of whom had normal brain function, whereas 2 had mild cerebral dysfunction. To improve prognostication in patients with initially successful resuscitation, Bayes' theorem was applied using 4 clinical findings after 24 hours' treatment: reactions to painful stimuli, pupillary size, light reactions and BP, Bayes' theorem as well as coma depth after 24 hours gave valuable information regarding individual prognosis."}
{"id": "PMID:484259", "title": "Mortality, arrhythmias and pump failure in acute myocardial infarction in relation to estimated infarct size.", "content": "Serial estimations of total serum creatine kinase (S-CK) were made in 194 consecutive patients with acute myocardial infarction (AMI). By itself, the maximum CK value could not separate patients in terms of high and low mortality but when the maximum CK value was related to age for patients with and without a history of previous AMI, two subgroups became apparent, one with 46% mortality (high-risk group) and another with 6% (low-risk group) during the hospital stay plus the next 90 days. In 114 of the patients, infarct size could be calculated. A good correlation was found between maximum CK and calculated infarct size (r = 0.93). Calculated infarct size alone could not distinguish between high and low mortality but when it was related to age for patients with and without a history of previous AMI, two subgroups emerged, one with 43% mortality and another with 3% during the hospital stay plus the next 90 days. The incidence of ventricular tachycardia during the stay in the Coronary Care Unit did not differ between the two risk groups separated either by maximum CK value or calculated infarct size. However, the incidence of shock and severe left heart failure during the acute phase was higher in the high-risk groups.", "contents": "Mortality, arrhythmias and pump failure in acute myocardial infarction in relation to estimated infarct size. Serial estimations of total serum creatine kinase (S-CK) were made in 194 consecutive patients with acute myocardial infarction (AMI). By itself, the maximum CK value could not separate patients in terms of high and low mortality but when the maximum CK value was related to age for patients with and without a history of previous AMI, two subgroups became apparent, one with 46% mortality (high-risk group) and another with 6% (low-risk group) during the hospital stay plus the next 90 days. In 114 of the patients, infarct size could be calculated. A good correlation was found between maximum CK and calculated infarct size (r = 0.93). Calculated infarct size alone could not distinguish between high and low mortality but when it was related to age for patients with and without a history of previous AMI, two subgroups emerged, one with 43% mortality and another with 3% during the hospital stay plus the next 90 days. The incidence of ventricular tachycardia during the stay in the Coronary Care Unit did not differ between the two risk groups separated either by maximum CK value or calculated infarct size. However, the incidence of shock and severe left heart failure during the acute phase was higher in the high-risk groups."}
{"id": "PMID:484260", "title": "The Q-T syndrome--a family description.", "content": "This paper describes a family of nine siblings of whom five suffer from the surdocardiac syndrome. All five are deaf-mute and have medical histories typical of the syndrome, with frequent syncopal attacks during the childhood, often caused by stress. Two of these five siblings have not suffered more than single attacks since puberty and are still alive. The other three had had continuous frequent attacks into adult years and died in connection with syncopes at 20, 27 and 37 years of age. The diagnosis, pathogenesis, treatment and genetics of the syndrome are discussed.", "contents": "The Q-T syndrome--a family description. This paper describes a family of nine siblings of whom five suffer from the surdocardiac syndrome. All five are deaf-mute and have medical histories typical of the syndrome, with frequent syncopal attacks during the childhood, often caused by stress. Two of these five siblings have not suffered more than single attacks since puberty and are still alive. The other three had had continuous frequent attacks into adult years and died in connection with syncopes at 20, 27 and 37 years of age. The diagnosis, pathogenesis, treatment and genetics of the syndrome are discussed."}
{"id": "PMID:484261", "title": "A controlled study of early discharge after uncomplicated myocardial infarction.", "content": "Out of 383 myocardial infarction (MI) patients aged below 70 years, 252 (66%) were judged after the third day in hospital to have had uncomplicated infarctions. These patients were allocated at random to two groups, one of which was given treatment for 8 days and the other for 15 days. No significant differences in mortality, morbidity or incapacity for work could be detected during the three-month period of follow-up. The findings thus support previous conclusions that early discharge from hospital after uncomplicated MI is not associated with greater risk for the patient than later discharge.", "contents": "A controlled study of early discharge after uncomplicated myocardial infarction. Out of 383 myocardial infarction (MI) patients aged below 70 years, 252 (66%) were judged after the third day in hospital to have had uncomplicated infarctions. These patients were allocated at random to two groups, one of which was given treatment for 8 days and the other for 15 days. No significant differences in mortality, morbidity or incapacity for work could be detected during the three-month period of follow-up. The findings thus support previous conclusions that early discharge from hospital after uncomplicated MI is not associated with greater risk for the patient than later discharge."}
{"id": "PMID:484262", "title": "Comparison of streptokinase with heparin: late results in the treatment of deep venous thrombosis.", "content": "Nineteen cases were reinvestigated 8-14 years after treatment with SK or heparin. Judging from personal interviews, foot volumometry and phlebography, treatment with SK appears to be more preferable since it was less often followed by late severe postthrombotic changes.", "contents": "Comparison of streptokinase with heparin: late results in the treatment of deep venous thrombosis. Nineteen cases were reinvestigated 8-14 years after treatment with SK or heparin. Judging from personal interviews, foot volumometry and phlebography, treatment with SK appears to be more preferable since it was less often followed by late severe postthrombotic changes."}
{"id": "PMID:484263", "title": "Blood pressure in 60-year-old men. Findings in a health survey and some comparisons with 50-year-old men in the same community.", "content": "A population survey of 60-year-old men (n=331) was performed in Uppsala. tthe prevalence of hypertension defined as established hypertension and those having an unknown diastolic blood pressure (DBP) of greater than or equal to 105 mmHg was 19.3%. Of the hypertensive population, 35.9% had not been detected previously. In the treated group, 43.9% were poorly controlled regarding the BP level. Thus 64.1% of the total hypertensive population at the age of 60 years were either undetected or inadequately controlled. Excretion of urinary electrolytes was also evaluated in this work. All participants of the health survey except four performed a 24-hour collection of urine following the health examination. Care was taken with endogenous creatinine clearance. Negative and significant correlations were noted between excretion of sodium and potassium in urine and DBP levels in the group (n=23) of untreated hypertensives. In a population sample (n=135) of healthy subjects devoid of medical treatment in the same population survey, a positive and significant correlation was noted between the systolic BP and the urinary excretion of sodium. Thus up to a certain BP level there is a pressure diuresis and at higher BP levels the kidney retains electrolytes.", "contents": "Blood pressure in 60-year-old men. Findings in a health survey and some comparisons with 50-year-old men in the same community. A population survey of 60-year-old men (n=331) was performed in Uppsala. tthe prevalence of hypertension defined as established hypertension and those having an unknown diastolic blood pressure (DBP) of greater than or equal to 105 mmHg was 19.3%. Of the hypertensive population, 35.9% had not been detected previously. In the treated group, 43.9% were poorly controlled regarding the BP level. Thus 64.1% of the total hypertensive population at the age of 60 years were either undetected or inadequately controlled. Excretion of urinary electrolytes was also evaluated in this work. All participants of the health survey except four performed a 24-hour collection of urine following the health examination. Care was taken with endogenous creatinine clearance. Negative and significant correlations were noted between excretion of sodium and potassium in urine and DBP levels in the group (n=23) of untreated hypertensives. In a population sample (n=135) of healthy subjects devoid of medical treatment in the same population survey, a positive and significant correlation was noted between the systolic BP and the urinary excretion of sodium. Thus up to a certain BP level there is a pressure diuresis and at higher BP levels the kidney retains electrolytes."}
{"id": "PMID:484264", "title": "Two- and three-dimensional hexon crystals.", "content": "Soluble hexon of type 1 adenovirus was highly purified with different techniques and dialysed against 0.5 M acetate buffer. With this procedure tetrahedral crystals were produced from the soluble hexon capsomers of the virus capsid. Electron microscopic observation of the crystallization process, revealed the development of dense two-dimensional \"crystal sheets\" following dialysis, in homogeneous hexon preparations containing single hexons. No such formations were observed so far with other types. The occurrence of two-dimensional crystals decreased proportionally to the appearance of three-dimensional crystals, which refers to their possible role in the mechanism of three-dimensional crystal formation.", "contents": "Two- and three-dimensional hexon crystals. Soluble hexon of type 1 adenovirus was highly purified with different techniques and dialysed against 0.5 M acetate buffer. With this procedure tetrahedral crystals were produced from the soluble hexon capsomers of the virus capsid. Electron microscopic observation of the crystallization process, revealed the development of dense two-dimensional \"crystal sheets\" following dialysis, in homogeneous hexon preparations containing single hexons. No such formations were observed so far with other types. The occurrence of two-dimensional crystals decreased proportionally to the appearance of three-dimensional crystals, which refers to their possible role in the mechanism of three-dimensional crystal formation."}
{"id": "PMID:484265", "title": "Toxoplasma antibodies in ophthalmological patients.", "content": "Sera of 532 patients suffering from different ophthalmological diseases were studied for the presence of toxoplasma antibodies. Complement fixation test was positive in 44.9% of the patients. As compared with the 26.8% positivity rate determined earlier in the normal population the seropositivity in eye-patients was significantly higher. The highest incidence, 50%, was found in the age group of 21--30 years. In diseases with a confirmed aetiological role of toxoplasmosis, the incidence of seropositivity was higher than in the total material. This suggests that Toxoplasma gondii may have had a role in part of the diseases of the patients studied.", "contents": "Toxoplasma antibodies in ophthalmological patients. Sera of 532 patients suffering from different ophthalmological diseases were studied for the presence of toxoplasma antibodies. Complement fixation test was positive in 44.9% of the patients. As compared with the 26.8% positivity rate determined earlier in the normal population the seropositivity in eye-patients was significantly higher. The highest incidence, 50%, was found in the age group of 21--30 years. In diseases with a confirmed aetiological role of toxoplasmosis, the incidence of seropositivity was higher than in the total material. This suggests that Toxoplasma gondii may have had a role in part of the diseases of the patients studied."}
{"id": "PMID:484266", "title": "Dianhydrodulcitol treatment of lymphocytic choriomeningitis virus infection in suckling mice.", "content": "Mice 2--4 days of age were pretreated with a single 5 mg/kg dose of dianhydrodulcitol (DAD) and later infected intracerebrally with lymphocytic choriomeningitis (LCM) virus. These animals had a lower mortality rate and died later than the untreated control animals. Thus DAD pretreatment prevented in part of the animals the development of lethal meningitis, the consequence of LCM virus infection, reducing the cellular immune response. This effect of DAD could equally be observed in animals infected at the age of 16--18 days and of 4 weeks.", "contents": "Dianhydrodulcitol treatment of lymphocytic choriomeningitis virus infection in suckling mice. Mice 2--4 days of age were pretreated with a single 5 mg/kg dose of dianhydrodulcitol (DAD) and later infected intracerebrally with lymphocytic choriomeningitis (LCM) virus. These animals had a lower mortality rate and died later than the untreated control animals. Thus DAD pretreatment prevented in part of the animals the development of lethal meningitis, the consequence of LCM virus infection, reducing the cellular immune response. This effect of DAD could equally be observed in animals infected at the age of 16--18 days and of 4 weeks."}
{"id": "PMID:484267", "title": "Adaptation of the influenza neuraminidase and neuraminidase-inhibition assays to the microtitrator system.", "content": "A micro-method based on Warren's colour reaction has been developed for influenza neuraminidase and neuraminidase-inhibition assays. Extraction with butanol is omitted and the assay is performed in wells of special plexiglass trays, where the reaction mixon and compared to controls in the same tray. The micro-assay, being inexpensive, time-saving and easy to perform even in poorly equipped laboratories, is suitable for large-scale serological screening and identification of the neuraminidase antigen of isolates.", "contents": "Adaptation of the influenza neuraminidase and neuraminidase-inhibition assays to the microtitrator system. A micro-method based on Warren's colour reaction has been developed for influenza neuraminidase and neuraminidase-inhibition assays. Extraction with butanol is omitted and the assay is performed in wells of special plexiglass trays, where the reaction mixon and compared to controls in the same tray. The micro-assay, being inexpensive, time-saving and easy to perform even in poorly equipped laboratories, is suitable for large-scale serological screening and identification of the neuraminidase antigen of isolates."}
{"id": "PMID:484268", "title": "Incidence of non-cholera vibrios in Hungary.", "content": "In the period 1971 to 1976, 200 non-cholera vibrio (NCV) strains were isolated in Hungary; 18 of the cultures were derived from 34 729 faecal and 182 from 237 surface water samples. Ninety-two strains belonged to the Heiberg-Smith group I and 108 to group II. Two strains failed to give the string test and 3 were pteridine resistant. The strains were classified into 48 serotypes according to Smith's system. Faecal NCV strains belonged to serotypes 46 and 328; these serotypes did not occur in water. Of the 18 faecal strains 13 were isolated from 18 048 persons who had travelled in cholera-infected areas, and 5 strains from persons who had never left Hungary (2 from 4559 patients with diarrhoea and 3 from 6061 healthy individuals). These data indicate that although NCV are present in the environment, they play an insignificant role in enteric infections in Hungary.", "contents": "Incidence of non-cholera vibrios in Hungary. In the period 1971 to 1976, 200 non-cholera vibrio (NCV) strains were isolated in Hungary; 18 of the cultures were derived from 34 729 faecal and 182 from 237 surface water samples. Ninety-two strains belonged to the Heiberg-Smith group I and 108 to group II. Two strains failed to give the string test and 3 were pteridine resistant. The strains were classified into 48 serotypes according to Smith's system. Faecal NCV strains belonged to serotypes 46 and 328; these serotypes did not occur in water. Of the 18 faecal strains 13 were isolated from 18 048 persons who had travelled in cholera-infected areas, and 5 strains from persons who had never left Hungary (2 from 4559 patients with diarrhoea and 3 from 6061 healthy individuals). These data indicate that although NCV are present in the environment, they play an insignificant role in enteric infections in Hungary."}
{"id": "PMID:484269", "title": "Middle ear infection due to vibrio alginolyticus. Bacteriological characterization.", "content": "A 47-year-old male suffering from chronic purulent ear discharge was investigated bacteriologically in autumn 1977. The isolated strain was identified as Vibrio alginolyticus. The fact that the patient spent his vacation at a Black-Sea resort every year strongly pleads for a direct inoculation of the halophilic Vibrio.", "contents": "Middle ear infection due to vibrio alginolyticus. Bacteriological characterization. A 47-year-old male suffering from chronic purulent ear discharge was investigated bacteriologically in autumn 1977. The isolated strain was identified as Vibrio alginolyticus. The fact that the patient spent his vacation at a Black-Sea resort every year strongly pleads for a direct inoculation of the halophilic Vibrio."}
{"id": "PMID:484270", "title": "Proprioceptive influences on directional hearing: dependence on the movement type.", "content": "Proprioceptive effects (PE) on directional hearing, i.e., sound image shifts relatively to the sound source, produced by changes in head position, were studied in their dependence on head movement type in two groups of listeners. The first group included subjects in whom horizontal head deviation to the right or to the left, performed on instruction before the presentation of clicks, caused sound image displacement of 10-15 degrees. When the same sounds were made signals of movement direction, i.e., of pointing with the head at the sound source, PE became fully inhibited in all subjects. The second group included listeners in whom PE was either absent or only very small; substitution of passive head deviations for active turns resulted (in 63 percent of cases) in PEs which presumably had been previously inhibited. Thus identical muscle activities play different roles in auditory spatial perception depending on the type of movement and on the significance of the acoustic signal for the motor act.", "contents": "Proprioceptive influences on directional hearing: dependence on the movement type. Proprioceptive effects (PE) on directional hearing, i.e., sound image shifts relatively to the sound source, produced by changes in head position, were studied in their dependence on head movement type in two groups of listeners. The first group included subjects in whom horizontal head deviation to the right or to the left, performed on instruction before the presentation of clicks, caused sound image displacement of 10-15 degrees. When the same sounds were made signals of movement direction, i.e., of pointing with the head at the sound source, PE became fully inhibited in all subjects. The second group included listeners in whom PE was either absent or only very small; substitution of passive head deviations for active turns resulted (in 63 percent of cases) in PEs which presumably had been previously inhibited. Thus identical muscle activities play different roles in auditory spatial perception depending on the type of movement and on the significance of the acoustic signal for the motor act."}
{"id": "PMID:484271", "title": "Avoidance acquisition in cats as a function of temporal and intensity factors.", "content": "Acquisition of an active bar-pressing avoidance response was investigated in a factorial design with 3, 5 or 9 s CS-US intervals and CSs of 70- or 50-dB of white noise. As the CS-US interval increased the required time to meet the avoidance criterion decreased. The shorter the CS-US interval and the more intense the CS, the shorter the median response latencies observed at the end of the training. The latency data were analyzed in Vincentized fifths of acquisition trials. At the beginning of training, when the highest proportion of responses occurred soon after the US onset, escape latencies were shorter in groups trained with shorter CS-US intervals and the more intense CS. During the 1st and 2nd fifths of training, avoidances occurred with the same probability in each part the CS-US interval. By the middle of the training, after a performance level of 50 percent avoidance responses was reached, a rapid increase in the number of short-latency avoidance was observed, which was more pronounced in groups trained with the 70 dB CS. While the general shape of the latency distributions was preserved during the remaining two fifths of the training, a further increase in the intensity effect was noted, which occurred along with a decrease in the CS-US interval effect. The data are in favour of Two-factor Theory of Avoidance learning and suggest that at various stages of training, different variables emerge for acquisition and consolidation of the avoidance reflex. Data supported the hypothesis that two different mechanisms are involved in performance of short- and long-latency avoidance responses.", "contents": "Avoidance acquisition in cats as a function of temporal and intensity factors. Acquisition of an active bar-pressing avoidance response was investigated in a factorial design with 3, 5 or 9 s CS-US intervals and CSs of 70- or 50-dB of white noise. As the CS-US interval increased the required time to meet the avoidance criterion decreased. The shorter the CS-US interval and the more intense the CS, the shorter the median response latencies observed at the end of the training. The latency data were analyzed in Vincentized fifths of acquisition trials. At the beginning of training, when the highest proportion of responses occurred soon after the US onset, escape latencies were shorter in groups trained with shorter CS-US intervals and the more intense CS. During the 1st and 2nd fifths of training, avoidances occurred with the same probability in each part the CS-US interval. By the middle of the training, after a performance level of 50 percent avoidance responses was reached, a rapid increase in the number of short-latency avoidance was observed, which was more pronounced in groups trained with the 70 dB CS. While the general shape of the latency distributions was preserved during the remaining two fifths of the training, a further increase in the intensity effect was noted, which occurred along with a decrease in the CS-US interval effect. The data are in favour of Two-factor Theory of Avoidance learning and suggest that at various stages of training, different variables emerge for acquisition and consolidation of the avoidance reflex. Data supported the hypothesis that two different mechanisms are involved in performance of short- and long-latency avoidance responses."}
{"id": "PMID:484272", "title": "A system of command neurons in snail's escape behavior.", "content": "Intracellular stimulation of each of the nine identified neurons released a specific withdrawal reactions. These cells fire in response to strong tactile, light and thermal stimuli releasing escape behavior, and are assumed to be command neurons.", "contents": "A system of command neurons in snail's escape behavior. Intracellular stimulation of each of the nine identified neurons released a specific withdrawal reactions. These cells fire in response to strong tactile, light and thermal stimuli releasing escape behavior, and are assumed to be command neurons."}
{"id": "PMID:484273", "title": "Optic nerve gliomas. II. Cytological characteristics. Observations on cell and tissue cultures.", "content": "Cytological characteristics of optic nerve gliomas were studied parallel with histological structures in cell and tissue cultures. Histologically the tumours consisted of four different cell types: spindle-shaped elongated cells, round cells, stellate forms, and multinucleated cells. Dynamic properties of cultured optic glioma cells showed that stable cellular elements of the parent tissue arise from two fundamental populations, viz from elongated (piloid), and from round cells. Cells of both populations displayed a series of changes in the course of their cultures. A certain part of the slender elongated cells enlarged and became plump piloid cells of various shapes, or big stellate astrocytes. Giant multinucleated variants of these forms also emerged by fusion of the mononucleate forms. The round cells gradually turned into immature Astrocytes, and by a subsequent cell fusion they gave birth to big stellate cells. With the ageing of the cultures a polymorphous cellular picture developed which was mainly due to the active migratory and intracellular movement of the elongated and round cellular elements. Mitoses played a negligible role in this process. Several characteristics suggest that the round shapes are immature glia cells, and represent the least differentiated elements among all the cell components. The stellate and giant multinucleated forms might be considered as end-products of glia cell differentiation and they could originate both from the elongated and the round cells.", "contents": "Optic nerve gliomas. II. Cytological characteristics. Observations on cell and tissue cultures. Cytological characteristics of optic nerve gliomas were studied parallel with histological structures in cell and tissue cultures. Histologically the tumours consisted of four different cell types: spindle-shaped elongated cells, round cells, stellate forms, and multinucleated cells. Dynamic properties of cultured optic glioma cells showed that stable cellular elements of the parent tissue arise from two fundamental populations, viz from elongated (piloid), and from round cells. Cells of both populations displayed a series of changes in the course of their cultures. A certain part of the slender elongated cells enlarged and became plump piloid cells of various shapes, or big stellate astrocytes. Giant multinucleated variants of these forms also emerged by fusion of the mononucleate forms. The round cells gradually turned into immature Astrocytes, and by a subsequent cell fusion they gave birth to big stellate cells. With the ageing of the cultures a polymorphous cellular picture developed which was mainly due to the active migratory and intracellular movement of the elongated and round cellular elements. Mitoses played a negligible role in this process. Several characteristics suggest that the round shapes are immature glia cells, and represent the least differentiated elements among all the cell components. The stellate and giant multinucleated forms might be considered as end-products of glia cell differentiation and they could originate both from the elongated and the round cells."}
{"id": "PMID:484274", "title": "Schwannoma of the sellar region.", "content": "A 25-year-old woman who had experienced brief periods of loss of consciousness and grand mal seizures was found to have a midline mass in the sellar region as evidenced by computer tomography and angiography. Preoperatively, it was thought to be a meningioma but histologically and ultrastructurally it turned out to be a Schwannoma. Since cranial nerves were not involved, clinically or morphologically, this nerve sheath tumour could have originated from Schwann cells of sensory, possibly trigeminal, nerves, vasomotor nerves, or ectopic Schwann cells.", "contents": "Schwannoma of the sellar region. A 25-year-old woman who had experienced brief periods of loss of consciousness and grand mal seizures was found to have a midline mass in the sellar region as evidenced by computer tomography and angiography. Preoperatively, it was thought to be a meningioma but histologically and ultrastructurally it turned out to be a Schwannoma. Since cranial nerves were not involved, clinically or morphologically, this nerve sheath tumour could have originated from Schwann cells of sensory, possibly trigeminal, nerves, vasomotor nerves, or ectopic Schwann cells."}
{"id": "PMID:484275", "title": "Persistent embryonic veins in the arteriovenous malformations of the dura.", "content": "The basic anatomy of the arteriovenous malformations of the dura is similar to that of the internal carotid (pial) malformations, as the arterial feeders in both these lesions arise from the internal carotid artery in the embryo. The venous drainage occurs through definite channels into the major venous sinuses. Therefore, the dural and the pial malformations should be classified together. The scalp malformations have no features similar to these groups. The prominent occipital artery is not necessarily the primary feeder. Whenever a prominent occipital artery is seen, special efforts must be made to locate the primary dural feeders arising from the internal carotid artery.", "contents": "Persistent embryonic veins in the arteriovenous malformations of the dura. The basic anatomy of the arteriovenous malformations of the dura is similar to that of the internal carotid (pial) malformations, as the arterial feeders in both these lesions arise from the internal carotid artery in the embryo. The venous drainage occurs through definite channels into the major venous sinuses. Therefore, the dural and the pial malformations should be classified together. The scalp malformations have no features similar to these groups. The prominent occipital artery is not necessarily the primary feeder. Whenever a prominent occipital artery is seen, special efforts must be made to locate the primary dural feeders arising from the internal carotid artery."}
{"id": "PMID:484276", "title": "Subarachnoid haemorrhage of unknown aetiology.", "content": "One hundred and forty consecutive subarachnoid haemorrhages (SAH) which presented either an intracranial vascular malformation (102 cases: aneurysm 80 cases, AVH 22 cases) or remained of \"unknown aetiology\" (38 cases) were studied. SAH caused by other factors (neoplasms, thrombo-embolisms, systemic diseases etc.) were excluded. The 38 cases with bleeding of \"unknown aetiology\" have been studied by complete cerebral angiography, pneumoencephalogram or CT scan, and have been followed for two years. The most important clinical factors in the three groups have been compared by a statistical method to verify the hypothesis that SAH of \"unknown aetiology\" is caused by vascular micromalformations which are angiographically not evident either because of their small size or because of their \"spontaneous\" recovery after bleeding due to thrombosis and disappearance of the malformation. From the data collected it may be concluded that SAH of \"unknown aetiology\" is a benign lesion, typically occurring in middle age, but with no clear characteristics that enable identification of the anatomical substratum from it originates.", "contents": "Subarachnoid haemorrhage of unknown aetiology. One hundred and forty consecutive subarachnoid haemorrhages (SAH) which presented either an intracranial vascular malformation (102 cases: aneurysm 80 cases, AVH 22 cases) or remained of \"unknown aetiology\" (38 cases) were studied. SAH caused by other factors (neoplasms, thrombo-embolisms, systemic diseases etc.) were excluded. The 38 cases with bleeding of \"unknown aetiology\" have been studied by complete cerebral angiography, pneumoencephalogram or CT scan, and have been followed for two years. The most important clinical factors in the three groups have been compared by a statistical method to verify the hypothesis that SAH of \"unknown aetiology\" is caused by vascular micromalformations which are angiographically not evident either because of their small size or because of their \"spontaneous\" recovery after bleeding due to thrombosis and disappearance of the malformation. From the data collected it may be concluded that SAH of \"unknown aetiology\" is a benign lesion, typically occurring in middle age, but with no clear characteristics that enable identification of the anatomical substratum from it originates."}
{"id": "PMID:484277", "title": "Chronic bilateral subdural haematoma in adults.", "content": "Twenty-nine patients with chronic bilateral subdural haematomas were surgically treated during 1966 to 1977. Twenty-four of them (83%) had a history of head injury, which caused unconsciousness in eight cases. The mean interval from trauma to operation was eleven weeks. The mean age of the patients was 60 years. The prevalence of the most commonly encountered symptoms and signs was: headache 72%, mental symptoms 48%, papilloedema 41%, vertigo 31%, nausea 28%, reduced consciousness 28%, walking difficulties 24%, hemiparesis 24%, and paraparesis 14%. The aggregate thickness of haematomas was 34 mm, 36 mm, and 40 mm in age groups of 20--39, 40--59, and over 60 years, respectively. All patients were operated on, four of them only unilaterally. Three patients in the whole series died. Two of them had been operated upon only on one side in the first session, the haematoma of the other side being evacuated 8 1/2 hours and four days later, respectively. Unilateral operation is likely to cause severe distortion of the midline structures and the brain stem and thus aggravates the cerebral situation. Therefore the necessity of simultaneous evacuation of the haematomas on both sides is stressed. The reason for the death of the third patient was delay in diagnosis. All three patients who died belonged to the group of eight patients with a reduced level of consciousness before surgery. Twenty-three of the survivors were fully independent in their daily lives, and three needed some help after operative treatment.", "contents": "Chronic bilateral subdural haematoma in adults. Twenty-nine patients with chronic bilateral subdural haematomas were surgically treated during 1966 to 1977. Twenty-four of them (83%) had a history of head injury, which caused unconsciousness in eight cases. The mean interval from trauma to operation was eleven weeks. The mean age of the patients was 60 years. The prevalence of the most commonly encountered symptoms and signs was: headache 72%, mental symptoms 48%, papilloedema 41%, vertigo 31%, nausea 28%, reduced consciousness 28%, walking difficulties 24%, hemiparesis 24%, and paraparesis 14%. The aggregate thickness of haematomas was 34 mm, 36 mm, and 40 mm in age groups of 20--39, 40--59, and over 60 years, respectively. All patients were operated on, four of them only unilaterally. Three patients in the whole series died. Two of them had been operated upon only on one side in the first session, the haematoma of the other side being evacuated 8 1/2 hours and four days later, respectively. Unilateral operation is likely to cause severe distortion of the midline structures and the brain stem and thus aggravates the cerebral situation. Therefore the necessity of simultaneous evacuation of the haematomas on both sides is stressed. The reason for the death of the third patient was delay in diagnosis. All three patients who died belonged to the group of eight patients with a reduced level of consciousness before surgery. Twenty-three of the survivors were fully independent in their daily lives, and three needed some help after operative treatment."}
{"id": "PMID:484279", "title": "Examination of the metabolism of oedematous brain tissue. V. The metabolism of oedematous white matter from rabbit brain.", "content": "Glucose and gas metabolism as well as water, sodium, and potassium content of normal and oedematous white matter slices of rabbit brain have been determined. A cutting technique for the preparation of the slices is described. Although the increase of the relative water content in oedematous white matter is much more pronounced than in oedema of the grey matter, we observed only one significant change: the pyruvate production in vitro is about 20% higher in slices prepared from oedematous brain.", "contents": "Examination of the metabolism of oedematous brain tissue. V. The metabolism of oedematous white matter from rabbit brain. Glucose and gas metabolism as well as water, sodium, and potassium content of normal and oedematous white matter slices of rabbit brain have been determined. A cutting technique for the preparation of the slices is described. Although the increase of the relative water content in oedematous white matter is much more pronounced than in oedema of the grey matter, we observed only one significant change: the pyruvate production in vitro is about 20% higher in slices prepared from oedematous brain."}
{"id": "PMID:484278", "title": "Acquired hydrocephalus. V. Determination of the formation rate of albumin in the ventricular system.", "content": "By quantitative isotope ventriculography (QIV), the formation rate of albumin in the ventricular system (FRalb) was determined in 14 hydrocephalic and 14 nonhydrocephalic patients. The FRalb was significantly reduced in the patients with acquired hydrocephalus. By a comparison of the mean formation rate of albumin (MFRalb) and the mean absorption rate of albumin (MARalb), a significant biological difference in the two groups of patients was demonstrated.", "contents": "Acquired hydrocephalus. V. Determination of the formation rate of albumin in the ventricular system. By quantitative isotope ventriculography (QIV), the formation rate of albumin in the ventricular system (FRalb) was determined in 14 hydrocephalic and 14 nonhydrocephalic patients. The FRalb was significantly reduced in the patients with acquired hydrocephalus. By a comparison of the mean formation rate of albumin (MFRalb) and the mean absorption rate of albumin (MARalb), a significant biological difference in the two groups of patients was demonstrated."}
{"id": "PMID:484312", "title": "Sexual experimentation by adolescents while babysitting.", "content": "This paper deals with the sexual experiences an adolescent might have as a result of babysitting. The impact of babysitters in our culture has been ignored in the literature, and yet we find it practiced widely by teenagers in the United States. Parents and society in general have few guidelines in sitter selection and in recognizing the potential impact of the sitter on their children.", "contents": "Sexual experimentation by adolescents while babysitting. This paper deals with the sexual experiences an adolescent might have as a result of babysitting. The impact of babysitters in our culture has been ignored in the literature, and yet we find it practiced widely by teenagers in the United States. Parents and society in general have few guidelines in sitter selection and in recognizing the potential impact of the sitter on their children."}
{"id": "PMID:484313", "title": "Encopresis in adolescence: two case studies.", "content": "Encopresis is an underreported psychopathological symptom of adolescence, not necessarily defining a specific diagnostic entity. The two cases presented offer an opportunity to evaluate encopresis occurring in markedly different adolescent pathological entities and developmental backgrounds. The first patient presented a longitudinal life course wherein toilet training and fecal considerations were prominent throughout his development. Indeed, this young man had such areas of cohesive functioning, as to be appropriately considered within the range of characterological pathology, severe, though it may be. In marked contrast, the second patient's encopresis represented but a small part of a totally encompassing psychotic disintegration.", "contents": "Encopresis in adolescence: two case studies. Encopresis is an underreported psychopathological symptom of adolescence, not necessarily defining a specific diagnostic entity. The two cases presented offer an opportunity to evaluate encopresis occurring in markedly different adolescent pathological entities and developmental backgrounds. The first patient presented a longitudinal life course wherein toilet training and fecal considerations were prominent throughout his development. Indeed, this young man had such areas of cohesive functioning, as to be appropriately considered within the range of characterological pathology, severe, though it may be. In marked contrast, the second patient's encopresis represented but a small part of a totally encompassing psychotic disintegration."}
{"id": "PMID:484315", "title": "Self-injury behavior, economic status and the family anomie syndrome among adolescents.", "content": "Only recently has there been a focus upon the psychosocial environment of the family that surrounds the suicide-prone individual. The purpose of this research was to learn more about the association of economic status and family anomie, operationationalized by family normlessness and powerlessness scales, to the differential vulnerability of adolescents to suicide potential. The research design called for a detailed interview with the persons attempting suicide and members of their families. The study samples consisted of 30 low economic and 25 high economic status families. It was predicted that social anomie could be translated into behavioral (attempted suicide) and attitudinal (normlessness and powerlessness) determinants when viewed with regard to its impact upon the family. Significant differences in the degree of normlessness and powerlessness were found for suicidal and nonsuicidal adolescents and their families. These differences appear to be part of a family anomie syndrome.", "contents": "Self-injury behavior, economic status and the family anomie syndrome among adolescents. Only recently has there been a focus upon the psychosocial environment of the family that surrounds the suicide-prone individual. The purpose of this research was to learn more about the association of economic status and family anomie, operationationalized by family normlessness and powerlessness scales, to the differential vulnerability of adolescents to suicide potential. The research design called for a detailed interview with the persons attempting suicide and members of their families. The study samples consisted of 30 low economic and 25 high economic status families. It was predicted that social anomie could be translated into behavioral (attempted suicide) and attitudinal (normlessness and powerlessness) determinants when viewed with regard to its impact upon the family. Significant differences in the degree of normlessness and powerlessness were found for suicidal and nonsuicidal adolescents and their families. These differences appear to be part of a family anomie syndrome."}
{"id": "PMID:484317", "title": "Siblings as babysitters: responses of adolescents to younger siblings in problem situations.", "content": "Adolescents (75 males, 150 females) assumed the role of parent surrogate and responded to nine problem situations involving younger siblings. Responses were coded into 21 categories--grouped as high, medium, low and non-power as well as \"effective\" or \"ineffective\"--and analyzed by sex, grade (10, 11, or 12) and birth order (first-, second-, or laterborn). Ninety-two percent of responses were categorized as \"ineffective\" (i.e., not communicating own or sibling's feelings). Seniors used less high and more medium and non-power responses than younger students. Most students, particularly females, expressed interest in joining a group to learn better ways of relating to younger siblings.", "contents": "Siblings as babysitters: responses of adolescents to younger siblings in problem situations. Adolescents (75 males, 150 females) assumed the role of parent surrogate and responded to nine problem situations involving younger siblings. Responses were coded into 21 categories--grouped as high, medium, low and non-power as well as \"effective\" or \"ineffective\"--and analyzed by sex, grade (10, 11, or 12) and birth order (first-, second-, or laterborn). Ninety-two percent of responses were categorized as \"ineffective\" (i.e., not communicating own or sibling's feelings). Seniors used less high and more medium and non-power responses than younger students. Most students, particularly females, expressed interest in joining a group to learn better ways of relating to younger siblings."}
{"id": "PMID:484318", "title": "The Anti-Social Activities Attitude Scale.", "content": "A scale was developed to measure attitudes toward anti-social activities for adolescents. Reliability coefficients were greater than .9. Validity was determined by comparing two known groups.", "contents": "The Anti-Social Activities Attitude Scale. A scale was developed to measure attitudes toward anti-social activities for adolescents. Reliability coefficients were greater than .9. Validity was determined by comparing two known groups."}
{"id": "PMID:484341", "title": "Meniere's disease: a neuropsychological study II.", "content": "23 Meniere patients were examined with an extensive battery of neuropsychological tests. In a previous investigation the authors had demonstrated that patients with a long duration of Meniere's disease had psychological disturbances, presumably localized in the nondominant hemisphere. In the present investigation the patients had a short duration of the disease, and we were unable to reproduce the psychological disturbances. It is concluded that the central changes in the nondominant hemisphere function develop over a long period of time. It is hoped for that further neuropsychological investigations of Meniere patients can give more information of what is primary in Meniere's disease: peripheral sensory or central neurological changes.", "contents": "Meniere's disease: a neuropsychological study II. 23 Meniere patients were examined with an extensive battery of neuropsychological tests. In a previous investigation the authors had demonstrated that patients with a long duration of Meniere's disease had psychological disturbances, presumably localized in the nondominant hemisphere. In the present investigation the patients had a short duration of the disease, and we were unable to reproduce the psychological disturbances. It is concluded that the central changes in the nondominant hemisphere function develop over a long period of time. It is hoped for that further neuropsychological investigations of Meniere patients can give more information of what is primary in Meniere's disease: peripheral sensory or central neurological changes."}
{"id": "PMID:484342", "title": "Epidemiological survey of definite cases of Meniere's disease collected by the seventeen members of the Meniere's Disease Research Committee of Japan in 1975--1976.", "content": "Between Aril 1975 and December 1976, the second nationwide survey of Meniere's disease in Japan was made by the 17 members of the Meniere's Disease Research Committee of Japan. The epidemiological data from 520 patients with definite Meniere's disease were analyzed in comparison with those from the 126 patients in the non-Meniere vertiginous group (Control A) and the 228 patients in the rhinolaryngological group (Control B). The male:female ratio of definite cases of Meniere's disease was almost the same, and the age distribution peaked at the age group of 40--49 years for males, while the peak for females was at the age group of 30--39 years. 5.8% of the 520 patients had a close relative who also suffered from Meniere's disease. From the epidemiological features, it may appear that the occurrence of vertiginous attacks in Meniere's disease is influenced much more by individual than by environmental factors. However, this feature can be considered as another evidence of the psychosomatic disorders involved in Meniere's disease.", "contents": "Epidemiological survey of definite cases of Meniere's disease collected by the seventeen members of the Meniere's Disease Research Committee of Japan in 1975--1976. Between Aril 1975 and December 1976, the second nationwide survey of Meniere's disease in Japan was made by the 17 members of the Meniere's Disease Research Committee of Japan. The epidemiological data from 520 patients with definite Meniere's disease were analyzed in comparison with those from the 126 patients in the non-Meniere vertiginous group (Control A) and the 228 patients in the rhinolaryngological group (Control B). The male:female ratio of definite cases of Meniere's disease was almost the same, and the age distribution peaked at the age group of 40--49 years for males, while the peak for females was at the age group of 30--39 years. 5.8% of the 520 patients had a close relative who also suffered from Meniere's disease. From the epidemiological features, it may appear that the occurrence of vertiginous attacks in Meniere's disease is influenced much more by individual than by environmental factors. However, this feature can be considered as another evidence of the psychosomatic disorders involved in Meniere's disease."}
{"id": "PMID:484343", "title": "Bilateral Meniere's disease.", "content": "A survey of 265 Japanese patients with Meniere's disease revealed bilateral involvement in approximately 29% of all these patients. Somatic and psychiatric aspects of bilateral Meniere's disease were as follows. (1) The duration of the disease in cases of bilateral involvement was significantly longer than cases of unilateral involvement. (2) An abnormal general condition, a more extensive degree of hearing loss and neurotic type were frequently diagnosed in cases of bilateral involvement. Based on these results, it was concluded that psychiatric management and prevention of aggravation of deafness should be part of the management of treatment in bilateral Meniere's disease.", "contents": "Bilateral Meniere's disease. A survey of 265 Japanese patients with Meniere's disease revealed bilateral involvement in approximately 29% of all these patients. Somatic and psychiatric aspects of bilateral Meniere's disease were as follows. (1) The duration of the disease in cases of bilateral involvement was significantly longer than cases of unilateral involvement. (2) An abnormal general condition, a more extensive degree of hearing loss and neurotic type were frequently diagnosed in cases of bilateral involvement. Based on these results, it was concluded that psychiatric management and prevention of aggravation of deafness should be part of the management of treatment in bilateral Meniere's disease."}
{"id": "PMID:484344", "title": "Measurement of eye deviation under the nonfixative condition in patients with peripheral labyrinthine disorders, especially Meniere's disease.", "content": "Among 232 Japanese patients with peripheral disorders, 98 had a unilateral Meniere's disease. In these patients and 20 healthy adults, recordings of eye deviation were made utilizing DC-ENG under two conditions, i.e. eyes closed (EC) and eyes open in the absolute darkness (AD). (1) 90 % tolerance interval of AD. was significantly smaller than that of EC in controls. (2) Appearance of nystagmus in 5 groups with labyrinthine lesions was compared with the direction of deviation toward and against the affected side in both conditions. (3) Concerning unilateral Meniere's, the relationship among deviation, nystagmus and caloric response was analyzed statistically and the clinical application discussed.", "contents": "Measurement of eye deviation under the nonfixative condition in patients with peripheral labyrinthine disorders, especially Meniere's disease. Among 232 Japanese patients with peripheral disorders, 98 had a unilateral Meniere's disease. In these patients and 20 healthy adults, recordings of eye deviation were made utilizing DC-ENG under two conditions, i.e. eyes closed (EC) and eyes open in the absolute darkness (AD). (1) 90 % tolerance interval of AD. was significantly smaller than that of EC in controls. (2) Appearance of nystagmus in 5 groups with labyrinthine lesions was compared with the direction of deviation toward and against the affected side in both conditions. (3) Concerning unilateral Meniere's, the relationship among deviation, nystagmus and caloric response was analyzed statistically and the clinical application discussed."}
{"id": "PMID:484345", "title": "The ultrastructure of the endolymphatic sac in Meniere's disease.", "content": "Biopsies of the endolymphatic sac were performed in 6 patients undergoing endolymphatic sac decompression and/or drainage procedures. Electron microscopy revealed extensive subendothelial fibrosis with loss of vascularity, heavy deposition of collagen and flattening of luminal cells. In patients with long-standing disease, complete obliteration of the endolymphatic sac lumen appeared to occur. There was poor correlation between the surgeon's ability to identify the lumen of the endolymphatic sac and its presence in the biopsy specimen.", "contents": "The ultrastructure of the endolymphatic sac in Meniere's disease. Biopsies of the endolymphatic sac were performed in 6 patients undergoing endolymphatic sac decompression and/or drainage procedures. Electron microscopy revealed extensive subendothelial fibrosis with loss of vascularity, heavy deposition of collagen and flattening of luminal cells. In patients with long-standing disease, complete obliteration of the endolymphatic sac lumen appeared to occur. There was poor correlation between the surgeon's ability to identify the lumen of the endolymphatic sac and its presence in the biopsy specimen."}
{"id": "PMID:484346", "title": "Caloric nystagmus: ENG in comparison with observation by Frenzel's glasses.", "content": "After caloric stimulation with water at 30 degrees C for 30 sec an electronystagmogram (ENG) was registered. Simultaneously with the ENG the test person's nystagmus was observed by Frenzel's glasses. If the evaluation is limited to the frequency of the nystagmus and if it is possible to neglect the velocity of the slow phase, the simple observation through Frenzel's glasses and its plotting on a time chart recorder is no less effective than an ENG for measuring the difference between the right and left labyrinth.", "contents": "Caloric nystagmus: ENG in comparison with observation by Frenzel's glasses. After caloric stimulation with water at 30 degrees C for 30 sec an electronystagmogram (ENG) was registered. Simultaneously with the ENG the test person's nystagmus was observed by Frenzel's glasses. If the evaluation is limited to the frequency of the nystagmus and if it is possible to neglect the velocity of the slow phase, the simple observation through Frenzel's glasses and its plotting on a time chart recorder is no less effective than an ENG for measuring the difference between the right and left labyrinth."}
{"id": "PMID:484347", "title": "Dynamic evaluation of human vestibulo-ocular function using white noise rotation stimulus and linear system parameter estimation techniques.", "content": "White noise acceleration inputs were used to determine the human VOR transfer function both for normal subjects and for patients falling into two pilot categories: unilateral labyrinthectomy and reduced bilateral responses. The systematic patterns shown in the transfer function of the pilot abnormal categories as compared to the normal data suggests one method of classifying test results (table I). Frequency domain linear systems parameter fits were also made using the same data. The changes in these fit parameters, when pilot abnormal data is compared to normal data, suggests the use of the parameter fits themselves as a second classification scheme (fig. 1). The second scheme is not appropriate in cases where the response is unrelated to the stimulus.", "contents": "Dynamic evaluation of human vestibulo-ocular function using white noise rotation stimulus and linear system parameter estimation techniques. White noise acceleration inputs were used to determine the human VOR transfer function both for normal subjects and for patients falling into two pilot categories: unilateral labyrinthectomy and reduced bilateral responses. The systematic patterns shown in the transfer function of the pilot abnormal categories as compared to the normal data suggests one method of classifying test results (table I). Frequency domain linear systems parameter fits were also made using the same data. The changes in these fit parameters, when pilot abnormal data is compared to normal data, suggests the use of the parameter fits themselves as a second classification scheme (fig. 1). The second scheme is not appropriate in cases where the response is unrelated to the stimulus."}
{"id": "PMID:484348", "title": "Some critical remarks on the interpretation of vestibular test results.", "content": "In the scope of the evaluation of vestibular function, the attention is drawn to the fact that results of tests are to be considered in a relative way. They do not express the absolute functional capacity, but in half of the cases it can be shown that the functional state is intensity dependent. This can be observed in claoric tests as well as in rotation tests. In these latter even the type of stimulation may give a different result. The experiences obtained in a number of cases by both stimulation types confirm this assessment that the functional result is always to be considered in this relative way. This implies some reserves as to the comparability of results of various authors, using different stimulation schemes. On the other hand, and this especially in rotation tests, application of more stimulation intensities allows to separate cases with stable from those with labile results.", "contents": "Some critical remarks on the interpretation of vestibular test results. In the scope of the evaluation of vestibular function, the attention is drawn to the fact that results of tests are to be considered in a relative way. They do not express the absolute functional capacity, but in half of the cases it can be shown that the functional state is intensity dependent. This can be observed in claoric tests as well as in rotation tests. In these latter even the type of stimulation may give a different result. The experiences obtained in a number of cases by both stimulation types confirm this assessment that the functional result is always to be considered in this relative way. This implies some reserves as to the comparability of results of various authors, using different stimulation schemes. On the other hand, and this especially in rotation tests, application of more stimulation intensities allows to separate cases with stable from those with labile results."}
{"id": "PMID:484349", "title": "Accumulation of substances with melanin affinity to the internal ear. Therapeutic or ototoxic mechanism?", "content": "The capacity of the melanin in the internal ear to accumulate and retain labelled lidocaine, bupivacaine and chlorpromazine after intravenous and intraperitoneal injection was examined by whole-body autoradiography. Both young pigmented hooded rats and albino rats were studied. In the pigmented rats chlorpromazine showed the greatest accumulation, which was more pronounced in the cochlea than in the vestibular portion. The other two substances were evenly distributed in the internal ear. After a single injection of chlorpromazine and of bupivacaine these substances were still bound to the melanin of the internal ear after 14 days, which was the longest survival time. Lidocaine, on the other hand, had disappeared after only 4 days. In albino animals there was very weak, transient uptake of chlorpromazine and bupivacaine, but not of lidocaine, in the internal ear. In studies in vitro on isolated bovine eye melanin there was considerably greater adsorption of chlorpromazine than of lidocaine and bupivacaine.", "contents": "Accumulation of substances with melanin affinity to the internal ear. Therapeutic or ototoxic mechanism? The capacity of the melanin in the internal ear to accumulate and retain labelled lidocaine, bupivacaine and chlorpromazine after intravenous and intraperitoneal injection was examined by whole-body autoradiography. Both young pigmented hooded rats and albino rats were studied. In the pigmented rats chlorpromazine showed the greatest accumulation, which was more pronounced in the cochlea than in the vestibular portion. The other two substances were evenly distributed in the internal ear. After a single injection of chlorpromazine and of bupivacaine these substances were still bound to the melanin of the internal ear after 14 days, which was the longest survival time. Lidocaine, on the other hand, had disappeared after only 4 days. In albino animals there was very weak, transient uptake of chlorpromazine and bupivacaine, but not of lidocaine, in the internal ear. In studies in vitro on isolated bovine eye melanin there was considerably greater adsorption of chlorpromazine than of lidocaine and bupivacaine."}
{"id": "PMID:484350", "title": "Vestibular neuronal function during ischemia. Response of vestibular neurons to vertebral and carotid artery occlusion in rabbits.", "content": "The relation between ischemia experimentally produced by occlusion of arteries injecting to the brain and the changes in vestibular excitability was examined in 102 rabbit vestibular neurons responding to sinusoidal rotation. During occlusion of ipsilateral and/or contralateral vertebral and common carotid arteries, the neuronal activity took on three different patterns with respect to the frequency of neuronal discharge. The incidence of alteration of neuronal activities reflected vestibular excitability dependent on hemodynamic changes due to occlusion of the ipsilateral vertebral artery rather than due to occlusion of the contralateral artery. Occlusion of common carotid arteries caused no significant difference between the right and left vessels in the resultant change of the neuronal activity, and differed little, either, from occlusion of vertebral arteries in the incidence of the changes. A supplementary experiment, in which the four arteries were only partially occluded, clearly showed that the resultant changes in neuronal activity reflect the role of Willis' circle in the maintenance of blood flow to vestibular nuclei.", "contents": "Vestibular neuronal function during ischemia. Response of vestibular neurons to vertebral and carotid artery occlusion in rabbits. The relation between ischemia experimentally produced by occlusion of arteries injecting to the brain and the changes in vestibular excitability was examined in 102 rabbit vestibular neurons responding to sinusoidal rotation. During occlusion of ipsilateral and/or contralateral vertebral and common carotid arteries, the neuronal activity took on three different patterns with respect to the frequency of neuronal discharge. The incidence of alteration of neuronal activities reflected vestibular excitability dependent on hemodynamic changes due to occlusion of the ipsilateral vertebral artery rather than due to occlusion of the contralateral artery. Occlusion of common carotid arteries caused no significant difference between the right and left vessels in the resultant change of the neuronal activity, and differed little, either, from occlusion of vertebral arteries in the incidence of the changes. A supplementary experiment, in which the four arteries were only partially occluded, clearly showed that the resultant changes in neuronal activity reflect the role of Willis' circle in the maintenance of blood flow to vestibular nuclei."}
{"id": "PMID:484351", "title": "Low-frequency harmonic acceleration in the evaluation of surgical treatment of Meniere's disease.", "content": "23 patients with classical findings of Meniere's disease and so diagnosed by a neurotologist were tested with harmonic acceleration. 7 of these patients were selected to receive endolymphatic-mastoid shunts for intractable vertigo and/or highly progressive fluctuant hearing loss. All 7 patients showed an increase in the impairment in their phase relationships at the lowest frequency (0.01Hz) following surgery which was considered to be secondary to the procedure. Later this phase lag returned to a level approaching normal. They also showed a definite decrease in the asymmetry (labyrinthine preponderance) of their nystagmic responses to acceleration.", "contents": "Low-frequency harmonic acceleration in the evaluation of surgical treatment of Meniere's disease. 23 patients with classical findings of Meniere's disease and so diagnosed by a neurotologist were tested with harmonic acceleration. 7 of these patients were selected to receive endolymphatic-mastoid shunts for intractable vertigo and/or highly progressive fluctuant hearing loss. All 7 patients showed an increase in the impairment in their phase relationships at the lowest frequency (0.01Hz) following surgery which was considered to be secondary to the procedure. Later this phase lag returned to a level approaching normal. They also showed a definite decrease in the asymmetry (labyrinthine preponderance) of their nystagmic responses to acceleration."}
{"id": "PMID:484352", "title": "Optokinetic nystagmus in artificial hemianopsy.", "content": "Optokinetic nystagmus in cases of artificial hemianopsy was studied. Hemianopsy was produced by using a combination of a projector, an erasing device and DC ENG. The effect of the condition of foveal vision in hemianopsy, whether reserved or involved, was discussed. When foveal vision was reserved, nystagmus during stimulation in cases of hemianopsy was essentially the same as in the normal eye. In cases of hemianopsy in which foveal vision was involved, there was a remarkable difference between the mystagmus induced by foveofugal stimulation and that induced by foveopetal stimulation. In the former case the nystagmus was very fine and irregular, in spite of the fact that the nystagmus was well induced (it was nearly the same as in the normal eye in the latter case). Foveal vision is very important for the formation of the slow component of optokinetic nystagmus. Foveopetal movement of the image on the peripheral retina is very important for the formation of the quick component. Foveofugal movement on the peripheral retinal has, on the contrary, only a small effect in attracting the eye.", "contents": "Optokinetic nystagmus in artificial hemianopsy. Optokinetic nystagmus in cases of artificial hemianopsy was studied. Hemianopsy was produced by using a combination of a projector, an erasing device and DC ENG. The effect of the condition of foveal vision in hemianopsy, whether reserved or involved, was discussed. When foveal vision was reserved, nystagmus during stimulation in cases of hemianopsy was essentially the same as in the normal eye. In cases of hemianopsy in which foveal vision was involved, there was a remarkable difference between the mystagmus induced by foveofugal stimulation and that induced by foveopetal stimulation. In the former case the nystagmus was very fine and irregular, in spite of the fact that the nystagmus was well induced (it was nearly the same as in the normal eye in the latter case). Foveal vision is very important for the formation of the slow component of optokinetic nystagmus. Foveopetal movement of the image on the peripheral retina is very important for the formation of the quick component. Foveofugal movement on the peripheral retinal has, on the contrary, only a small effect in attracting the eye."}
{"id": "PMID:484353", "title": "The mechanism of inhibition of caloric nystagmus by eye closure.", "content": "24 normal adults and 324 clinical cases were examined, for the purpose of shedding light on the mechanism of inhibition of caloric nystagmus by eye closure. When the eyes were closed, an eye was suddenly adducted 13 +/- 5 degrees and elevated 55 +/- 11 degrees. These adductive and upward eye movements were normal responses caused by eye closure. Caloric nystagmus was inhibited by upward eye deviation. With the turning down of the vertical eye position, the caloric-nystagmus inhibition was released. During eye closure, there were four types of vertical eye-movement responses: (a) holding the eye position up, (b) fluctuation around an upward eye position, (c) gradual downward turning, (d) sudden downward turning, Types a, b and c were seen in normal subjects. However, type d was not seen in normal people.", "contents": "The mechanism of inhibition of caloric nystagmus by eye closure. 24 normal adults and 324 clinical cases were examined, for the purpose of shedding light on the mechanism of inhibition of caloric nystagmus by eye closure. When the eyes were closed, an eye was suddenly adducted 13 +/- 5 degrees and elevated 55 +/- 11 degrees. These adductive and upward eye movements were normal responses caused by eye closure. Caloric nystagmus was inhibited by upward eye deviation. With the turning down of the vertical eye position, the caloric-nystagmus inhibition was released. During eye closure, there were four types of vertical eye-movement responses: (a) holding the eye position up, (b) fluctuation around an upward eye position, (c) gradual downward turning, (d) sudden downward turning, Types a, b and c were seen in normal subjects. However, type d was not seen in normal people."}
{"id": "PMID:484354", "title": "The tonic oculomotor function of the cervical joint and muscle receptors.", "content": "Cases of peripheral labyrinthine lesions were examined by stimulation of the cervical joint and muscle receptors. Head-chin (HC) position to one side induced compensatory oculomotor tone (ipsilateral nystagmus), which depended on the function of the horizontal canal of that side. This compensatory tone increased the duration of the optokinetic after-nystagmus (OKAN), with stripes moving contralaterally to the HC position. Isometric contraction of the muscles which rotate the HC to one side induced ipsilateral oculomotor tone (contralateral nystagmus), which depended on the function of the utricle acting in the same direction. In one case, the muscle-induced tone increased the frequency of the OKN with the slow phase in the same direction; in another, it prolonged the slow-phase pursuit interval. In the absence of a vestibular component, distortion of the OKN was induced.", "contents": "The tonic oculomotor function of the cervical joint and muscle receptors. Cases of peripheral labyrinthine lesions were examined by stimulation of the cervical joint and muscle receptors. Head-chin (HC) position to one side induced compensatory oculomotor tone (ipsilateral nystagmus), which depended on the function of the horizontal canal of that side. This compensatory tone increased the duration of the optokinetic after-nystagmus (OKAN), with stripes moving contralaterally to the HC position. Isometric contraction of the muscles which rotate the HC to one side induced ipsilateral oculomotor tone (contralateral nystagmus), which depended on the function of the utricle acting in the same direction. In one case, the muscle-induced tone increased the frequency of the OKN with the slow phase in the same direction; in another, it prolonged the slow-phase pursuit interval. In the absence of a vestibular component, distortion of the OKN was induced."}
{"id": "PMID:484355", "title": "Calcium ion uptake and exchange in otoconia.", "content": "Calcium ion uptake into otoconia and bone mineral of the young adult rat was studied by use of 45Ca and liquid scintillation spectrometry. Results showed that saccular and utricular otoconia take up 45Ca in a time course generally comparable to bone but on a much lower scale. Saccular otoconia showed greater uptake than utricular. The results are interpreted to indicate the dynamic nature of saccular and utricular otoconia and their possible importance in preserving ionic homeostasis of the inferior and superior parts of the labyrinth, respectively.", "contents": "Calcium ion uptake and exchange in otoconia. Calcium ion uptake into otoconia and bone mineral of the young adult rat was studied by use of 45Ca and liquid scintillation spectrometry. Results showed that saccular and utricular otoconia take up 45Ca in a time course generally comparable to bone but on a much lower scale. Saccular otoconia showed greater uptake than utricular. The results are interpreted to indicate the dynamic nature of saccular and utricular otoconia and their possible importance in preserving ionic homeostasis of the inferior and superior parts of the labyrinth, respectively."}
{"id": "PMID:484356", "title": "The cochlear blood flow in relation to noise and cervical sympathectomy.", "content": "The effect of noise and unilateral transection of the cervical sympathetic trunk on cochlear blood flow was studied in anesthetized cats. The sound pressure level was 100 dB and the exposure time 6 min. Neither noise nor sympathectomy were found to affect the blood flow.", "contents": "The cochlear blood flow in relation to noise and cervical sympathectomy. The effect of noise and unilateral transection of the cervical sympathetic trunk on cochlear blood flow was studied in anesthetized cats. The sound pressure level was 100 dB and the exposure time 6 min. Neither noise nor sympathectomy were found to affect the blood flow."}
{"id": "PMID:484357", "title": "A critical evaluation of the glycerol test in Meniere's disease.", "content": "From the literature it is apparent that the glycerol test is being used frequently to evaluate Meniere patients, with regard to the choice of treatment, and in particular to find those who are suitable for surgery with different decompressive procedures. In our previous investigations on such patients we found that they often are extremely good placebo responders and it was natural to evaluate how important such psychological factors would be for the outcome of the glycerol test. In a group of patients with typical objective and subjective symptoms of Meniere's disease, the glycerol test was applied twice to each patient. In one of the tests, the patient was told that we would expect hearing to improve. In the other test, where the actual amount of glycerol was the same, we had the solution prepared with a different taste, and the patient was informed that we now would expect the hearing to deteriorate. The sequence of the two tests was randomly chosen for each patient. Most frequently the patient would follow our instructions, i.e. obtained a poorer hearing threshold when instructed to, and vice versa. The results are discussed with a special view to the risk of selection of suggestion-sensitive patients by this test.", "contents": "A critical evaluation of the glycerol test in Meniere's disease. From the literature it is apparent that the glycerol test is being used frequently to evaluate Meniere patients, with regard to the choice of treatment, and in particular to find those who are suitable for surgery with different decompressive procedures. In our previous investigations on such patients we found that they often are extremely good placebo responders and it was natural to evaluate how important such psychological factors would be for the outcome of the glycerol test. In a group of patients with typical objective and subjective symptoms of Meniere's disease, the glycerol test was applied twice to each patient. In one of the tests, the patient was told that we would expect hearing to improve. In the other test, where the actual amount of glycerol was the same, we had the solution prepared with a different taste, and the patient was informed that we now would expect the hearing to deteriorate. The sequence of the two tests was randomly chosen for each patient. Most frequently the patient would follow our instructions, i.e. obtained a poorer hearing threshold when instructed to, and vice versa. The results are discussed with a special view to the risk of selection of suggestion-sensitive patients by this test."}
{"id": "PMID:484358", "title": "Hearing improvement in attacks of Meniere's disease treated with pressure chamber.", "content": "A method to reduce endolymphatic pressure by exposing patients with Meniere's disease to underpressure has earlier been described. In this work the possible mechanisms are discussed. A material of 36 patients with acute attacks, treated in this way, is now presented. 15 out of these patients experienced a rapid hearing improvement. In 11 cases the remission has lasted for more than 1 year. The method seems to be of great value for those who improved, since at least an early permanent damage to the cochlea can be avoided.", "contents": "Hearing improvement in attacks of Meniere's disease treated with pressure chamber. A method to reduce endolymphatic pressure by exposing patients with Meniere's disease to underpressure has earlier been described. In this work the possible mechanisms are discussed. A material of 36 patients with acute attacks, treated in this way, is now presented. 15 out of these patients experienced a rapid hearing improvement. In 11 cases the remission has lasted for more than 1 year. The method seems to be of great value for those who improved, since at least an early permanent damage to the cochlea can be avoided."}
{"id": "PMID:484359", "title": "Cat primary canal neurons: relation of conduction velocity to resting and dynamic firing characteristics.", "content": "Spontaneous discharge patterns of first-order canal afferents were analyzed in cats anethetized with pentobarbital sodium with particular emphasis on the relationship of regularity of resting discharge, sensitivity to angular acceleration and adaptation to the time delay between electrical labyrinthine stimulation and recording from afferents near Scarpa's ganglion. Regular units were found to have a high resting rate, low sensitivity to angular acceleration, were mostly nonadapting during prolonged acceleration and showed relatively long latency to electrical stimulation. Irregular units tended to have a low resting rate, high sensitivity, frequently showed adaptation and had short latencies. Intermediate neurons had mixed characteristics of regular and irregular units. In medulated nerve fibers, a direct relation exists between conduction velocity and fiber diameter. As latency is due primarily to conduction in the first-order axon, we may speculate that regular neurons have thin fibers which innervate the slope of the crista, irregular neurons have thick fibers which innervate the summit, and intermediate units have medium caliber fibers which innervate both the slope and summit of the crista ampullaris.", "contents": "Cat primary canal neurons: relation of conduction velocity to resting and dynamic firing characteristics. Spontaneous discharge patterns of first-order canal afferents were analyzed in cats anethetized with pentobarbital sodium with particular emphasis on the relationship of regularity of resting discharge, sensitivity to angular acceleration and adaptation to the time delay between electrical labyrinthine stimulation and recording from afferents near Scarpa's ganglion. Regular units were found to have a high resting rate, low sensitivity to angular acceleration, were mostly nonadapting during prolonged acceleration and showed relatively long latency to electrical stimulation. Irregular units tended to have a low resting rate, high sensitivity, frequently showed adaptation and had short latencies. Intermediate neurons had mixed characteristics of regular and irregular units. In medulated nerve fibers, a direct relation exists between conduction velocity and fiber diameter. As latency is due primarily to conduction in the first-order axon, we may speculate that regular neurons have thin fibers which innervate the slope of the crista, irregular neurons have thick fibers which innervate the summit, and intermediate units have medium caliber fibers which innervate both the slope and summit of the crista ampullaris."}
{"id": "PMID:484360", "title": "Analysis of nonlinear afferent response properties from the guitarfish semicircular canal.", "content": "Nonlinear afferent response properties from the isolated guitarfish semicircular canal were investigated using a nonlinear system identification technique of combined high and low frequency rotational acceleration stimuli. Linear system unit impulse responses obtained from the dual inputs were systematically different relative to those obtained from low frequency stimuli only, implying nonlinear interactions. These results were attributed to neurophysiological mechanisms of phase locking and increased afferent excitability. A hypothesis was proposed in which similar nonlinear interactions could result from efferent activity in intact animals, suggesting that the efferents could act as a regulator of afferent response dynamics.", "contents": "Analysis of nonlinear afferent response properties from the guitarfish semicircular canal. Nonlinear afferent response properties from the isolated guitarfish semicircular canal were investigated using a nonlinear system identification technique of combined high and low frequency rotational acceleration stimuli. Linear system unit impulse responses obtained from the dual inputs were systematically different relative to those obtained from low frequency stimuli only, implying nonlinear interactions. These results were attributed to neurophysiological mechanisms of phase locking and increased afferent excitability. A hypothesis was proposed in which similar nonlinear interactions could result from efferent activity in intact animals, suggesting that the efferents could act as a regulator of afferent response dynamics."}
{"id": "PMID:484361", "title": "Genesis and maturation of vestibular hair cells.", "content": "The embryologic development in vivo and in vitro of mammalian hair cells in the crista ampullaris was continuously followed with regard to structural differentiation and maturation from the terminal mitosis to the morphological condition at partus or equivalent age in vitro. Otocysts were explanted both early and late during embryologic development: 13th and 16 gestation day, respectively. Both in fetal life and during in vitro conditions, the surface structures of the developing hair cell with regular arrangement of kinocilium/stereocilia were first differentiated, followed by a cytologic transformation intracellularly and subsequent development of nerve endings. Hair cells were able to develop without any morphologic contact with the nervous system. The afferent nerve system developed before the efferent nerve system (CBA/CBA mouse).", "contents": "Genesis and maturation of vestibular hair cells. The embryologic development in vivo and in vitro of mammalian hair cells in the crista ampullaris was continuously followed with regard to structural differentiation and maturation from the terminal mitosis to the morphological condition at partus or equivalent age in vitro. Otocysts were explanted both early and late during embryologic development: 13th and 16 gestation day, respectively. Both in fetal life and during in vitro conditions, the surface structures of the developing hair cell with regular arrangement of kinocilium/stereocilia were first differentiated, followed by a cytologic transformation intracellularly and subsequent development of nerve endings. Hair cells were able to develop without any morphologic contact with the nervous system. The afferent nerve system developed before the efferent nerve system (CBA/CBA mouse)."}
{"id": "PMID:484362", "title": "Height vertigo and human posture.", "content": "A theory is presented supporting a geometrical explanation for physiological height vertigo being a distance vertigo through visual destabilization of postural balance when the distance between the eyes and visible stationary contrasts becomes critically large. Physiological and posturographic data obtained under natural height vertigo conditions are consistent with this hypothesis.", "contents": "Height vertigo and human posture. A theory is presented supporting a geometrical explanation for physiological height vertigo being a distance vertigo through visual destabilization of postural balance when the distance between the eyes and visible stationary contrasts becomes critically large. Physiological and posturographic data obtained under natural height vertigo conditions are consistent with this hypothesis."}
{"id": "PMID:484411", "title": "Human development through occupation: theoretical frameworks in contemporary occupational therapy practice, part 1.", "content": "In 1968, the report of a conference sponsored by the American Occupational Therapy Association called for the development of an integrated theory of occupational therapy. A review of the practical problems faced by therapists today, contrasted with major developments in the art and science of the field during the past decade, suggests that this goal has not been attained. However, three major theoretical frameworks for therapy have evolved through the work of Fidler and Mosey, Wilbarger, and Llorens, who participated in the conference on theory. Reilly and associates developed a fourth framework of significance. Through analysis of theoretical constructs, generic concepts that characterize occupational therapy can be identified. Theoretical constructs and research validation of the four approaches to occupational therapy are discussed in this article. A second article to appear in a subsequent issue of AJOT illustrates the use of the four theoretical frameworks to derive a philosophical basis for practice and a model of the practice process called \"human development through occupation.\"", "contents": "Human development through occupation: theoretical frameworks in contemporary occupational therapy practice, part 1. In 1968, the report of a conference sponsored by the American Occupational Therapy Association called for the development of an integrated theory of occupational therapy. A review of the practical problems faced by therapists today, contrasted with major developments in the art and science of the field during the past decade, suggests that this goal has not been attained. However, three major theoretical frameworks for therapy have evolved through the work of Fidler and Mosey, Wilbarger, and Llorens, who participated in the conference on theory. Reilly and associates developed a fourth framework of significance. Through analysis of theoretical constructs, generic concepts that characterize occupational therapy can be identified. Theoretical constructs and research validation of the four approaches to occupational therapy are discussed in this article. A second article to appear in a subsequent issue of AJOT illustrates the use of the four theoretical frameworks to derive a philosophical basis for practice and a model of the practice process called \"human development through occupation.\""}
{"id": "PMID:484412", "title": "Upper extremity edema control: rationale of the techniques.", "content": "Edema of the upper extremity is a condition frequently encountered by those interested in hand rehabilitation. It frequently accompanies other problems associated with functional restrictions of the upper extremities. Its control and prevention are a part of the total rehabilitation of these extremities. Persistent edema becomes painful, disfiguring, and disabling. Fibrosis develops in the edematous tissue leading to contractures. The susceptibility for infection increases and the functional ability of the limb decreases. Treatment is based upon an understanding of the pathophysiology of edema. Edema occurs when there is an imbalance of effective pressures across the capillary membrane or when there is an obstruction to venous and lymphatic flow. The conservative treatment program consists of elevation, massage, use of external compression devices, exercises, and instructions to avoid contributing factors. This paper will discuss the physiologic rationale for the effectiveness of these treatment methods.", "contents": "Upper extremity edema control: rationale of the techniques. Edema of the upper extremity is a condition frequently encountered by those interested in hand rehabilitation. It frequently accompanies other problems associated with functional restrictions of the upper extremities. Its control and prevention are a part of the total rehabilitation of these extremities. Persistent edema becomes painful, disfiguring, and disabling. Fibrosis develops in the edematous tissue leading to contractures. The susceptibility for infection increases and the functional ability of the limb decreases. Treatment is based upon an understanding of the pathophysiology of edema. Edema occurs when there is an imbalance of effective pressures across the capillary membrane or when there is an obstruction to venous and lymphatic flow. The conservative treatment program consists of elevation, massage, use of external compression devices, exercises, and instructions to avoid contributing factors. This paper will discuss the physiologic rationale for the effectiveness of these treatment methods."}
{"id": "PMID:484417", "title": "Human development through occupation: a philosophy and conceptual model for practice, part 2.", "content": "The development of a philosophy of practice called \"human development through occupation\" is described in this paper. This philosophy was developed through study and identification of generic concepts in four major theoretical frameworks for occupational therapy described in a preceding article. The philosophy of practice proposes a view of Man, a view of human health, and a view of the profession. These theoretical and philosophical resources are then used to construct a conceptual model of the content and sequence of the occupational therapy practice process. It is believed that the four theoretical frameworks, philosophy of practice, and conceptual model detailed in these two papers can provide direction for general practitioners and specialists in service, education, and research. Examples of application are included, together with suggestions for further investigation.", "contents": "Human development through occupation: a philosophy and conceptual model for practice, part 2. The development of a philosophy of practice called \"human development through occupation\" is described in this paper. This philosophy was developed through study and identification of generic concepts in four major theoretical frameworks for occupational therapy described in a preceding article. The philosophy of practice proposes a view of Man, a view of human health, and a view of the profession. These theoretical and philosophical resources are then used to construct a conceptual model of the content and sequence of the occupational therapy practice process. It is believed that the four theoretical frameworks, philosophy of practice, and conceptual model detailed in these two papers can provide direction for general practitioners and specialists in service, education, and research. Examples of application are included, together with suggestions for further investigation."}
{"id": "PMID:484420", "title": "The overtranquilized patient.", "content": "When first seen, the overtranquilized patient may already be in withdrawal, may have other medical problems or may be seeking psychiatric help. The patient may, however, be primarily interested in obtaining more medication. Abuse and withdrawal of sedatives, tranquilizers, narcotics or alcohol, or a combination of these, present diagnostic pitfalls. Toxicologic studies are helpful in identifying the abused drug. The pentobarbital tolerance test is valuable in determining the daily dosage, stabilization and tapering. Follow-up is essential to patient care.", "contents": "The overtranquilized patient. When first seen, the overtranquilized patient may already be in withdrawal, may have other medical problems or may be seeking psychiatric help. The patient may, however, be primarily interested in obtaining more medication. Abuse and withdrawal of sedatives, tranquilizers, narcotics or alcohol, or a combination of these, present diagnostic pitfalls. Toxicologic studies are helpful in identifying the abused drug. The pentobarbital tolerance test is valuable in determining the daily dosage, stabilization and tapering. Follow-up is essential to patient care."}
{"id": "PMID:484421", "title": "Interpretation of the electroencephalogram.", "content": "The background pattern is described in terms of frequency and amplitude. Waves of varying frequencies may be normal or abnormal, depending on the patient's age and state of wakefulness. Specific features sought in the EEG are slowing of frequency, asymmetry of frequency or amplitude, paroxysmal disturbances, and spikes and sharp waves. Certain abnormalities may be present only after provocation by hyperventilation, sleep deprivation or induction, photic stimulation or drugs.", "contents": "Interpretation of the electroencephalogram. The background pattern is described in terms of frequency and amplitude. Waves of varying frequencies may be normal or abnormal, depending on the patient's age and state of wakefulness. Specific features sought in the EEG are slowing of frequency, asymmetry of frequency or amplitude, paroxysmal disturbances, and spikes and sharp waves. Certain abnormalities may be present only after provocation by hyperventilation, sleep deprivation or induction, photic stimulation or drugs."}
{"id": "PMID:484422", "title": "Myoglobinuria.", "content": "Myoglobinuria has a variety of causes, ranging from trauma to heavy exercise. Symptoms usually consist of myalgia and muscle weakness; dark, bloody-looking urine may be noted. Myoglobin can be identified by readily available laboratory techniques. Acute renal failure can be a dangerous consequence of myoglobinuria but, with rapid recognition and strict monitoring, the prognosis in most cases is good.", "contents": "Myoglobinuria. Myoglobinuria has a variety of causes, ranging from trauma to heavy exercise. Symptoms usually consist of myalgia and muscle weakness; dark, bloody-looking urine may be noted. Myoglobin can be identified by readily available laboratory techniques. Acute renal failure can be a dangerous consequence of myoglobinuria but, with rapid recognition and strict monitoring, the prognosis in most cases is good."}
{"id": "PMID:484423", "title": "Childhood automotive safety.", "content": "Automobile accidents are the number one killer of children. Effective devices for protecting infants and older children are now available. Counseling the parents on the proper use of car seats should begin in the prenatal period and should continue during regular checkups. Knowledge of the excuses that parents give for not using these devices can help offset some of the existing apathy. Family physicians are in an ideal position to provide proper preventive health counseling on the use of car restraints for children.", "contents": "Childhood automotive safety. Automobile accidents are the number one killer of children. Effective devices for protecting infants and older children are now available. Counseling the parents on the proper use of car seats should begin in the prenatal period and should continue during regular checkups. Knowledge of the excuses that parents give for not using these devices can help offset some of the existing apathy. Family physicians are in an ideal position to provide proper preventive health counseling on the use of car restraints for children."}
{"id": "PMID:484427", "title": "Ornithopulmonary disease.", "content": "Severe pulmonary infections in man may be caused by exposure to infected birds or their droppings. Ornithosis, histoplasmosis and cryptococcosis develop as a result of inhalation of an infective agent. Pigeon-breeder's lung is an allergic alveolitis caused by a hypersensitivity reaction to avian droppings or feathers. This condition is recurrent and is best treated by avoidance of further exposure. Corticosteroids are useful in acute cases.", "contents": "Ornithopulmonary disease. Severe pulmonary infections in man may be caused by exposure to infected birds or their droppings. Ornithosis, histoplasmosis and cryptococcosis develop as a result of inhalation of an infective agent. Pigeon-breeder's lung is an allergic alveolitis caused by a hypersensitivity reaction to avian droppings or feathers. This condition is recurrent and is best treated by avoidance of further exposure. Corticosteroids are useful in acute cases."}
{"id": "PMID:484429", "title": "Temporary atrial standstill.", "content": "Temporary atrial standstill is a relatively rare event requiring intraatrial electrogram recordings for its documentation. Previous reports have emphasized its relationship to drug intoxication, infarction, the immediate post open-heart surgery period, or the premorbid state; significant impairment of atrial excitability is usual. The occurrence of atrial standstill in the presence of intact atrial excitability suggests \"electrical isolation\" of the atria. The three patients we describe manifested temporary atrial standstill while maintaining atrial excitability. Diffuse conduction system disease and drug effects played a contributory role.", "contents": "Temporary atrial standstill. Temporary atrial standstill is a relatively rare event requiring intraatrial electrogram recordings for its documentation. Previous reports have emphasized its relationship to drug intoxication, infarction, the immediate post open-heart surgery period, or the premorbid state; significant impairment of atrial excitability is usual. The occurrence of atrial standstill in the presence of intact atrial excitability suggests \"electrical isolation\" of the atria. The three patients we describe manifested temporary atrial standstill while maintaining atrial excitability. Diffuse conduction system disease and drug effects played a contributory role."}
{"id": "PMID:484430", "title": "Surgical treatment of anomalous left coronary artery from pulmonary artery: follow-up in teenagers and adults.", "content": "To determine which method of surgical therapy might be optimal for patients with anomalous left coronary artery from the pulmonary artery (ALCAPA), a follow-up study was performed. Twenty-nine teenagers and adults who had ALCAPA diagnosed during life at age 13 years or older were identified mainly by literature search. Recent follow-up was obtained on all. Thirteen treated by ALCAPA ligation alone (Group A), were followed a mean of 9.2 years postoperately (range 1 to 15 years). There was no operative mortality. Three Group A patients died suddenly; a mean of five years (range 2 to 7 years) postoperatively. Sixteen patients treated by simultaneous ALCAPA ligation and saphenous vein graft (SVG) from aorta to left coronary artery (Group B) were followed a mean of five years (range 0 to 11 years) with one intraoperative death and no late mortality. Using the generalized Wilcoxon test for single censored samples, there was no significant difference in survival at any postoperative year when comparing both Groups A and B. The late appearance of sudden death in three Group A patients and no late deaths in Group B patients suggests that ligation and SVG, or its equivalent, may be the therapy of choice.", "contents": "Surgical treatment of anomalous left coronary artery from pulmonary artery: follow-up in teenagers and adults. To determine which method of surgical therapy might be optimal for patients with anomalous left coronary artery from the pulmonary artery (ALCAPA), a follow-up study was performed. Twenty-nine teenagers and adults who had ALCAPA diagnosed during life at age 13 years or older were identified mainly by literature search. Recent follow-up was obtained on all. Thirteen treated by ALCAPA ligation alone (Group A), were followed a mean of 9.2 years postoperately (range 1 to 15 years). There was no operative mortality. Three Group A patients died suddenly; a mean of five years (range 2 to 7 years) postoperatively. Sixteen patients treated by simultaneous ALCAPA ligation and saphenous vein graft (SVG) from aorta to left coronary artery (Group B) were followed a mean of five years (range 0 to 11 years) with one intraoperative death and no late mortality. Using the generalized Wilcoxon test for single censored samples, there was no significant difference in survival at any postoperative year when comparing both Groups A and B. The late appearance of sudden death in three Group A patients and no late deaths in Group B patients suggests that ligation and SVG, or its equivalent, may be the therapy of choice."}
{"id": "PMID:484433", "title": "Analysis of human atrial fibrillatory waves using monophasic action potential technique.", "content": "In 29 patients with atrial fibrillation, using the monophasic action potential technique, the fibrillatory waves were recorded and analyzed by statistical methods. Concerning the characteristics of the fibrillatory waves there are no differences between patients with rheumatic heart disease and with coronary heart disease. The patients with paroxysmal forms and those without heart failure and with normal heart size, have similar statistical data with regard to their fibrillatory waves. From the data presented, it could be possible to predict the efficacy of conversion to sinus rhythm and also the sinus rhythm stability after conversion.", "contents": "Analysis of human atrial fibrillatory waves using monophasic action potential technique. In 29 patients with atrial fibrillation, using the monophasic action potential technique, the fibrillatory waves were recorded and analyzed by statistical methods. Concerning the characteristics of the fibrillatory waves there are no differences between patients with rheumatic heart disease and with coronary heart disease. The patients with paroxysmal forms and those without heart failure and with normal heart size, have similar statistical data with regard to their fibrillatory waves. From the data presented, it could be possible to predict the efficacy of conversion to sinus rhythm and also the sinus rhythm stability after conversion."}
{"id": "PMID:484435", "title": "Use of oral prazosin hydrochloride in congestive failure following acute myocardial infarction.", "content": "The hemodynamic effects of the new oral vasocilator prazosin were evaluated in 11 patients with congestive failure following myocardial infarction. Prazosin decreased pulmonary trunk, pulmonary capillary, and right atrial pressures, systemic blood pressure, systemic vascular and total pulmonary resistances, and the heart rate X systolic blood pressure product (P less than 0.001). Cardiac and stroke indexes increased (P less than 0.001 and P less than 0.02, respectively). Heart rate, myocardial perfusion gradient, and stroke work index remained unchanged (P less than 0.05). Prazosin effect began in most patients 30 minutes after an oral administration of 2.5 to 7 mg; the maximum effect occurred from one to three hours later. The action is sustained for at least 6 hours in most patients and sometimes is present even 12 hours after a single dose administration. We conclude that prazosin, with a nitroprusside-like effect, improves left ventricular performance and myocardial O2 supply-demand relationship in patients with acute myocardial infarction, but optimal doses vary significantly, and the hazard of severe and sustained hypotension, or decline in cardiac output in patients with normal blood pressure or slightly elevated filling pressure, is present.", "contents": "Use of oral prazosin hydrochloride in congestive failure following acute myocardial infarction. The hemodynamic effects of the new oral vasocilator prazosin were evaluated in 11 patients with congestive failure following myocardial infarction. Prazosin decreased pulmonary trunk, pulmonary capillary, and right atrial pressures, systemic blood pressure, systemic vascular and total pulmonary resistances, and the heart rate X systolic blood pressure product (P less than 0.001). Cardiac and stroke indexes increased (P less than 0.001 and P less than 0.02, respectively). Heart rate, myocardial perfusion gradient, and stroke work index remained unchanged (P less than 0.05). Prazosin effect began in most patients 30 minutes after an oral administration of 2.5 to 7 mg; the maximum effect occurred from one to three hours later. The action is sustained for at least 6 hours in most patients and sometimes is present even 12 hours after a single dose administration. We conclude that prazosin, with a nitroprusside-like effect, improves left ventricular performance and myocardial O2 supply-demand relationship in patients with acute myocardial infarction, but optimal doses vary significantly, and the hazard of severe and sustained hypotension, or decline in cardiac output in patients with normal blood pressure or slightly elevated filling pressure, is present."}
{"id": "PMID:484436", "title": "Echocardiography and fetal heart sounds in the diagnosis of fetal heart block.", "content": "A 32-week fetus was demonstrated phonocardiographically and echocardiographically to have a regular atrial rate of 150 per minute and a regular ventricular rate of 39 per minute, indicating complete heart block. The diagnosis was suspected when two groups of heart sounds at two distinct rates were heard on auscultation, and was confirmed by the postnatal ECG. The maternal history was significant for the presence of systemic lupus erythematosus. The basis for the echocardiographic diatnosis of complete heart block, the presence of atrial heart sounds in complete heart block, and the relationship of maternal SLE to congenital heart block are discussed.", "contents": "Echocardiography and fetal heart sounds in the diagnosis of fetal heart block. A 32-week fetus was demonstrated phonocardiographically and echocardiographically to have a regular atrial rate of 150 per minute and a regular ventricular rate of 39 per minute, indicating complete heart block. The diagnosis was suspected when two groups of heart sounds at two distinct rates were heard on auscultation, and was confirmed by the postnatal ECG. The maternal history was significant for the presence of systemic lupus erythematosus. The basis for the echocardiographic diatnosis of complete heart block, the presence of atrial heart sounds in complete heart block, and the relationship of maternal SLE to congenital heart block are discussed."}
{"id": "PMID:484446", "title": "Local exhaust ventilation principles.", "content": "Many industrial local exhaust ventilation systems have inadequate performance. This occurs because systems were poorly designed. Frequently, the performance of the local exhaust ventilation systems is impaired because of modifications. Nine factors which should be considered when designing a local exhaust ventilation system include: 1. minimizing capture distance; 2. providing adequate capture velocity (or adequate exhaust rate); 3. using gravitational force; 4. providing adequate conveying velocity for particulates; 5. minimizing pressure drop; 6. incorporating \"containment\" whenever possible; 7. providing even airflow distribution at hood faces; 8. placing the fan as the last system element whenever possible; and 9. installing a static pressure gauge and testing the system.", "contents": "Local exhaust ventilation principles. Many industrial local exhaust ventilation systems have inadequate performance. This occurs because systems were poorly designed. Frequently, the performance of the local exhaust ventilation systems is impaired because of modifications. Nine factors which should be considered when designing a local exhaust ventilation system include: 1. minimizing capture distance; 2. providing adequate capture velocity (or adequate exhaust rate); 3. using gravitational force; 4. providing adequate conveying velocity for particulates; 5. minimizing pressure drop; 6. incorporating \"containment\" whenever possible; 7. providing even airflow distribution at hood faces; 8. placing the fan as the last system element whenever possible; and 9. installing a static pressure gauge and testing the system."}
{"id": "PMID:484447", "title": "A comprehensive program in asbestos hazard surveillance and education.", "content": "The surveillance program of asbestos workers at the Long Beach Naval Shipyard consists of a variety of clinical examinations and consultations; educational efforts targeted toward individual patients and groups; environmental measurement; maintenance of pertinent monographic and periodic literature; and records and report maintenance.", "contents": "A comprehensive program in asbestos hazard surveillance and education. The surveillance program of asbestos workers at the Long Beach Naval Shipyard consists of a variety of clinical examinations and consultations; educational efforts targeted toward individual patients and groups; environmental measurement; maintenance of pertinent monographic and periodic literature; and records and report maintenance."}
{"id": "PMID:484448", "title": "Size-selective preparation of inorganic fibers for biological experiments.", "content": "Size-selective procedures were developed for the preparation of fiber fractions of uniform size of chrysotile, crocidolite, amosite and glass. The raw material was first ground to a suitable fineness, then prescreened in a vibrating bed aerosol generator and finally suspended in liquids by ultrasonic agitation. Size-separation was then achieved by slow sedimentation. In addition, fiber fractions of exceedingly long-fibers were prepared by utilizing a vibrating sieve in the gas phase or in liquids.", "contents": "Size-selective preparation of inorganic fibers for biological experiments. Size-selective procedures were developed for the preparation of fiber fractions of uniform size of chrysotile, crocidolite, amosite and glass. The raw material was first ground to a suitable fineness, then prescreened in a vibrating bed aerosol generator and finally suspended in liquids by ultrasonic agitation. Size-separation was then achieved by slow sedimentation. In addition, fiber fractions of exceedingly long-fibers were prepared by utilizing a vibrating sieve in the gas phase or in liquids."}
{"id": "PMID:484449", "title": "Gas chromatography/mass spectrometry: its application to industrial hygiene analytical problems.", "content": "The mass spectrometer (MS), when used as a detector for the gas chromatography yields a system (GC/MS) which is compatible, sensitive, and provides specific information, suitable for qualitative or quantitative analyses for industrial hygiene samples. Examples of the use of two GC/MS/Data Systems utilizing industrial hygiene samples as encountered during the health hazard evaluation efforts of NIOSH (National Institute for Occupational Safety and Health) are presented. A quadrupole unit has been used as a service tool to analyze industrial field samples and a high resolution double focussing unit has been used in the areas of method development research.", "contents": "Gas chromatography/mass spectrometry: its application to industrial hygiene analytical problems. The mass spectrometer (MS), when used as a detector for the gas chromatography yields a system (GC/MS) which is compatible, sensitive, and provides specific information, suitable for qualitative or quantitative analyses for industrial hygiene samples. Examples of the use of two GC/MS/Data Systems utilizing industrial hygiene samples as encountered during the health hazard evaluation efforts of NIOSH (National Institute for Occupational Safety and Health) are presented. A quadrupole unit has been used as a service tool to analyze industrial field samples and a high resolution double focussing unit has been used in the areas of method development research."}
{"id": "PMID:484450", "title": "Workplace exposure zones for classification of employee exposures to physical and chemical agents.", "content": "A method involving classification of employee job tasks into exposure zones is proposed to guide the industrial hygienist in assigning personal samples to employees in a facility. Workers are assigned to an exposure zone on the basis of similar profiles of chemical exposures, ventilation characteristics, and job tasks. Two facilities are used to illustrate the power of the exposure zone method to reduce the number of required samples while increasing the confidence of identifying workers at high risk. Details of the zoning and calculation procedures are described in detail.", "contents": "Workplace exposure zones for classification of employee exposures to physical and chemical agents. A method involving classification of employee job tasks into exposure zones is proposed to guide the industrial hygienist in assigning personal samples to employees in a facility. Workers are assigned to an exposure zone on the basis of similar profiles of chemical exposures, ventilation characteristics, and job tasks. Two facilities are used to illustrate the power of the exposure zone method to reduce the number of required samples while increasing the confidence of identifying workers at high risk. Details of the zoning and calculation procedures are described in detail."}
{"id": "PMID:484451", "title": "Retrospective industrial hygiene surveys.", "content": "The true test of the long-term response of workers exposed to a hazardous agent is usually the epidemiological studies performed on same. This necessitates the long-term knowledge of exposure levels. Usually, the extent to which the exposures measured during recent experiences are representative of past exposures is difficult to assess as only limited historic measurements are available, and oftentimes the limited data are difficult to relate to today's measurement techniques. It is fallacious to assume that the conditions were worse in the past as it is fallacious to assume that they were better. The methodology of estimating past exposures to an agent based on industry-wide survey techniques is discussed.", "contents": "Retrospective industrial hygiene surveys. The true test of the long-term response of workers exposed to a hazardous agent is usually the epidemiological studies performed on same. This necessitates the long-term knowledge of exposure levels. Usually, the extent to which the exposures measured during recent experiences are representative of past exposures is difficult to assess as only limited historic measurements are available, and oftentimes the limited data are difficult to relate to today's measurement techniques. It is fallacious to assume that the conditions were worse in the past as it is fallacious to assume that they were better. The methodology of estimating past exposures to an agent based on industry-wide survey techniques is discussed."}
{"id": "PMID:484460", "title": "The clearance of cadmium aerosols after inhalation exposure.", "content": "CdO and CdCl2 aerosols were inhaled by two groups of rats and their subsequent clearance behaviour was studied. The half-life of the long term lung clearance rates was identical for both compounds, namely 67 days. For CdO, an initial faster clearance was observed, whereas the initial clearance of CdCl2 did not differ from its long term clearance rate, resulting in a relatively higher Cd burden of the lung in the CdCl2 study. Cd liver and kidney concentrations were also higher in the CdCl2 study, originating from a high resorption rate duringthe time of the exposure.", "contents": "The clearance of cadmium aerosols after inhalation exposure. CdO and CdCl2 aerosols were inhaled by two groups of rats and their subsequent clearance behaviour was studied. The half-life of the long term lung clearance rates was identical for both compounds, namely 67 days. For CdO, an initial faster clearance was observed, whereas the initial clearance of CdCl2 did not differ from its long term clearance rate, resulting in a relatively higher Cd burden of the lung in the CdCl2 study. Cd liver and kidney concentrations were also higher in the CdCl2 study, originating from a high resorption rate duringthe time of the exposure."}
{"id": "PMID:484461", "title": "Biomonitoring of industrial styrene exposures.", "content": "Worker exposure to styrene in two fiberglass boat plants was evaluated using conventional sampling techniques. The use of expired air and urine metabolite concentrations as indicators of styrene exposure is evaluated. The concentration of mandelic acid, a styrene metabolite in urine, is quantitated for workers without and with intermittent personal respiratory protection. A urinary Biological Limit Value is determined for exposures to the Threshold Limit Value of styrene.", "contents": "Biomonitoring of industrial styrene exposures. Worker exposure to styrene in two fiberglass boat plants was evaluated using conventional sampling techniques. The use of expired air and urine metabolite concentrations as indicators of styrene exposure is evaluated. The concentration of mandelic acid, a styrene metabolite in urine, is quantitated for workers without and with intermittent personal respiratory protection. A urinary Biological Limit Value is determined for exposures to the Threshold Limit Value of styrene."}
{"id": "PMID:484462", "title": "Benzene exposures during gasoline loading at bulk marketing terminals.", "content": "A study was conducted at 20 gasoline bulk marketing terminals to determine benzene exposure levels to both Gulf and outside carrier personnel operating at these facilities. Emphasis was placed on breathing zone samples of short duration during loading of trucks at the rack. Racks utilizing various methods of product transfer were surveyed. From the results of the study, it can be seen that the largest employee exposure to benzene occurs at facilities utilizing top loading without vapor recovery. Exposures during loading can exceed 0.5 ppm for an eight-hour time-weighted average.", "contents": "Benzene exposures during gasoline loading at bulk marketing terminals. A study was conducted at 20 gasoline bulk marketing terminals to determine benzene exposure levels to both Gulf and outside carrier personnel operating at these facilities. Emphasis was placed on breathing zone samples of short duration during loading of trucks at the rack. Racks utilizing various methods of product transfer were surveyed. From the results of the study, it can be seen that the largest employee exposure to benzene occurs at facilities utilizing top loading without vapor recovery. Exposures during loading can exceed 0.5 ppm for an eight-hour time-weighted average."}
{"id": "PMID:484463", "title": "Comparison of the aerodynamic size distribution of chain-like aggregates measured with a cascade impactor and a spiral centrifuge.", "content": "Several authors have suggested that chain-like aggregates fragment in the jets of cascade impactors and that the impactor consequently underestimates the median aerodynamic diameter and overestimates the geometric standard deviation (GSD). The aerodynamic size distribution of (Pu0.14U0.86)02.2 aggregates were measured simultaneously with two Mercer cascade impactors and a LAPS spiral centrifuge to investigate this problem. Although the median aerodynamic diameters were generally in good agreement, the impactor consistently measured higher values for the GSD than did the spiral centrifuge. Based on experiments in which losses in the centrifuge inlet were measured, it was concluded that the centrifuge had measured the aerodynamic size distribution more accurately than the impactor. Interception of chain-like aggregates on the first few impactor stages may have been the primary reason for the disparity between the impactor and centrifuge measurements.", "contents": "Comparison of the aerodynamic size distribution of chain-like aggregates measured with a cascade impactor and a spiral centrifuge. Several authors have suggested that chain-like aggregates fragment in the jets of cascade impactors and that the impactor consequently underestimates the median aerodynamic diameter and overestimates the geometric standard deviation (GSD). The aerodynamic size distribution of (Pu0.14U0.86)02.2 aggregates were measured simultaneously with two Mercer cascade impactors and a LAPS spiral centrifuge to investigate this problem. Although the median aerodynamic diameters were generally in good agreement, the impactor consistently measured higher values for the GSD than did the spiral centrifuge. Based on experiments in which losses in the centrifuge inlet were measured, it was concluded that the centrifuge had measured the aerodynamic size distribution more accurately than the impactor. Interception of chain-like aggregates on the first few impactor stages may have been the primary reason for the disparity between the impactor and centrifuge measurements."}
{"id": "PMID:484464", "title": "Fire fighter noise exposure.", "content": "Occupational noise exposure was evaluated for eight fire fighter positions on board three types of emergency vehicles. One hundred seventy code-3responses were monitored and sound pressure levels in excess of 115 dBA found. Futhermore, sound pressures exceeding the OSHA allowable level of 90 dBA for eight hours exposure were determined. Octave band analyses were performed for overall code-3 noise and for specific noise sources on each vehicle. An evaluation of eighty-nine audiograms of fire fighters was also carried out. The findings of this study suggest that under present operational conditions fire fighters experience excessive short-duration, high intensity noise exposure and a hearing conservation program for the fire service is therefore recommended.", "contents": "Fire fighter noise exposure. Occupational noise exposure was evaluated for eight fire fighter positions on board three types of emergency vehicles. One hundred seventy code-3responses were monitored and sound pressure levels in excess of 115 dBA found. Futhermore, sound pressures exceeding the OSHA allowable level of 90 dBA for eight hours exposure were determined. Octave band analyses were performed for overall code-3 noise and for specific noise sources on each vehicle. An evaluation of eighty-nine audiograms of fire fighters was also carried out. The findings of this study suggest that under present operational conditions fire fighters experience excessive short-duration, high intensity noise exposure and a hearing conservation program for the fire service is therefore recommended."}
{"id": "PMID:484465", "title": "Evaluation of dust exposure in asbestos cement manufacturing operations.", "content": "Previous investigations of the health of workers of the asbestos cement industry have demonstrated dose-response relationships for the diffuse radiographic changes of asbestosis and pulmonary function impairment. Studies of the mortality experience of past employees have also identified levels of cumulative exposure above which an excess of respiratory cancer could be detected. These epidemiologic studies were based on particulate concentrations determined by the midget impinger. We attempted to develop information that might lead to conversion of particulate to fiber concentrations in the industry in order that the risk assessment could be related to the current membrane filter methods. Pairs of impinger and membrane filter samples were taken in the various areas of the plant. Personal samples were also taken to determine the current exposure of workers. The ratio of fiber concentration to particle count varied from 0.63 to 2.5. Por correlation (0.18) was obtained at low fiber and particle counts and good correlation (0.91) was demonstrated in dustry areas where asbestos and silica were handled in a dry form. The concentration of fibers in 80% of the personal samples was less than 2 fibers/cc, and 60% were less than 0.5 fibers/cc. The results also show that no one conversion factor can be used for all areas of this type of operation.", "contents": "Evaluation of dust exposure in asbestos cement manufacturing operations. Previous investigations of the health of workers of the asbestos cement industry have demonstrated dose-response relationships for the diffuse radiographic changes of asbestosis and pulmonary function impairment. Studies of the mortality experience of past employees have also identified levels of cumulative exposure above which an excess of respiratory cancer could be detected. These epidemiologic studies were based on particulate concentrations determined by the midget impinger. We attempted to develop information that might lead to conversion of particulate to fiber concentrations in the industry in order that the risk assessment could be related to the current membrane filter methods. Pairs of impinger and membrane filter samples were taken in the various areas of the plant. Personal samples were also taken to determine the current exposure of workers. The ratio of fiber concentration to particle count varied from 0.63 to 2.5. Por correlation (0.18) was obtained at low fiber and particle counts and good correlation (0.91) was demonstrated in dustry areas where asbestos and silica were handled in a dry form. The concentration of fibers in 80% of the personal samples was less than 2 fibers/cc, and 60% were less than 0.5 fibers/cc. The results also show that no one conversion factor can be used for all areas of this type of operation."}
{"id": "PMID:484466", "title": "Correlation of fluorescence intensity and carcinogenic potency of synthetic and natural petroleums in mouse skin.", "content": "After standardized topical exposure of mouse skin to synthetic and natural petroleums, the penetration and persistence of the fluorescent components have been quantitated. Carcinogenicity of the same materials was determined by repeated skin application following by a period without treatment ot allow expression of malignantly transformed epidermal cells. No simple correlation was found for the materials between total specific fluorescence in either tissue or the test tube and their observed capacity to elicit skin necrosis or malignancy. The number of test materials was insufficient to allow generalization; however, the results do suggest that before fluorescence can be effectively employed as a tool for assessing potential occupational exposure to complex hydrocarbon mixtures, considerable refinement of the methodology will be required.", "contents": "Correlation of fluorescence intensity and carcinogenic potency of synthetic and natural petroleums in mouse skin. After standardized topical exposure of mouse skin to synthetic and natural petroleums, the penetration and persistence of the fluorescent components have been quantitated. Carcinogenicity of the same materials was determined by repeated skin application following by a period without treatment ot allow expression of malignantly transformed epidermal cells. No simple correlation was found for the materials between total specific fluorescence in either tissue or the test tube and their observed capacity to elicit skin necrosis or malignancy. The number of test materials was insufficient to allow generalization; however, the results do suggest that before fluorescence can be effectively employed as a tool for assessing potential occupational exposure to complex hydrocarbon mixtures, considerable refinement of the methodology will be required."}
{"id": "PMID:484467", "title": "Age effect hearing levels for a white nonindustrial noise exposed population (ninep) and their use in evaluating industrial hearing conservation programs.", "content": "A nonindustrial noise exposed population (NINEP) describing age effects for white males and females has been established that can be used as a reference in evaluating an industrial noise exposed population (INEP) data base. In making this comparison, it is desirable to match the two populations properly with respect to sex, race and age characteristics. Since there presently does not exist an equivalent black NINEP, it is necessary to first isolate the data representing the black population from the industrial sample. A definite learning curve exists in industrial audiometric data bases, with the degree of learning dependent upon the effectiveness of the hearing conservation program. Therefore, this variation must be considered when attempting to compare the industrial audiometric test data with the white NINEP data base presented herein.", "contents": "Age effect hearing levels for a white nonindustrial noise exposed population (ninep) and their use in evaluating industrial hearing conservation programs. A nonindustrial noise exposed population (NINEP) describing age effects for white males and females has been established that can be used as a reference in evaluating an industrial noise exposed population (INEP) data base. In making this comparison, it is desirable to match the two populations properly with respect to sex, race and age characteristics. Since there presently does not exist an equivalent black NINEP, it is necessary to first isolate the data representing the black population from the industrial sample. A definite learning curve exists in industrial audiometric data bases, with the degree of learning dependent upon the effectiveness of the hearing conservation program. Therefore, this variation must be considered when attempting to compare the industrial audiometric test data with the white NINEP data base presented herein."}
{"id": "PMID:484468", "title": "Monitoring personal exposure to ethylenediamine in the occupational environment.", "content": "A method for monitoring exposure to ethylenediamine (EDA) in the occupational environment is described. The EDA is adsorbed on activated silica gel, desorbed with 0.5 percent aqueous cupric chloride, and analyzed by gas chromatography using a 2 percent KOH on a Chromosorb 103 column. The method is sensitive to 200 micrograms/mL EDA and can detect 1.0 ppmv EDA in samples collected for 4.5 hours at a 300 cc per minute flow. The method has been evaluated in the laboratory and under plant conditions. Other amines do not interfere with the determination of EDA.", "contents": "Monitoring personal exposure to ethylenediamine in the occupational environment. A method for monitoring exposure to ethylenediamine (EDA) in the occupational environment is described. The EDA is adsorbed on activated silica gel, desorbed with 0.5 percent aqueous cupric chloride, and analyzed by gas chromatography using a 2 percent KOH on a Chromosorb 103 column. The method is sensitive to 200 micrograms/mL EDA and can detect 1.0 ppmv EDA in samples collected for 4.5 hours at a 300 cc per minute flow. The method has been evaluated in the laboratory and under plant conditions. Other amines do not interfere with the determination of EDA."}
{"id": "PMID:484469", "title": "Cotton dust sampling efficiency of the vertical elutriator.", "content": "A theoretical model of the vertical elutriator air sampler based upon the phenomenon of flow separation at the inlet was developed at SRRC. The model's equations were simulated by a computer program (VELUT). The model was calibrated by comparison of available experimental results to isokinetic sampling efficiencies calculated by the model. The calibrated model was used to predict integral efficiencies for a systematic assortment of size distributions of cotton dust. Rather than cutting off cleanly at 15 micrometers, the computer model exhibited a 50% differential efficiency at approximately 20 micrometers. Furthermore, the model predicted substantial non-zero collection efficiencies in the aerodynamic size range of individual cotton fibers. Program VELUT represents an objective method whereby the isokinetic sampling efficiency of the vertical elutriator can be explicitly calculated for any known or postulated ambient dust distribution presented to the sampler at its inlet plane.", "contents": "Cotton dust sampling efficiency of the vertical elutriator. A theoretical model of the vertical elutriator air sampler based upon the phenomenon of flow separation at the inlet was developed at SRRC. The model's equations were simulated by a computer program (VELUT). The model was calibrated by comparison of available experimental results to isokinetic sampling efficiencies calculated by the model. The calibrated model was used to predict integral efficiencies for a systematic assortment of size distributions of cotton dust. Rather than cutting off cleanly at 15 micrometers, the computer model exhibited a 50% differential efficiency at approximately 20 micrometers. Furthermore, the model predicted substantial non-zero collection efficiencies in the aerodynamic size range of individual cotton fibers. Program VELUT represents an objective method whereby the isokinetic sampling efficiency of the vertical elutriator can be explicitly calculated for any known or postulated ambient dust distribution presented to the sampler at its inlet plane."}
{"id": "PMID:484470", "title": "Potential hazard associated with scraping preparative thin layer chromatography plates.", "content": "Scraping TLC plates in a hood leads to contamination of the hood, equipment, worker, and the environment. The magnitude of contamination is presented as a fraction of the chemical delivered, and the effects of plate thickness, dryness, and adsorbent material are examined. Frequent clean-up and decontamination are recommended.", "contents": "Potential hazard associated with scraping preparative thin layer chromatography plates. Scraping TLC plates in a hood leads to contamination of the hood, equipment, worker, and the environment. The magnitude of contamination is presented as a fraction of the chemical delivered, and the effects of plate thickness, dryness, and adsorbent material are examined. Frequent clean-up and decontamination are recommended."}
{"id": "PMID:484471", "title": "Computerization of data from continuous and sequential air monitoring systems.", "content": "Continuous and sequential area air monitoring systems are more frequently becoming a necessity in both new and existing chemical manufacturing and processing plants. While these systems have most frequently been installed to alert employees to conditions of possible acute overexposure, they generate a wealth of data valuable in documenting employee exposures over extended period of time. Within the Dow Chemical Company, use of a dedicated microcomputer with area air monitoring systems has proven helful in summarizing each days' data and in calculating month-to-date totals for use in employee exposure documentation over long time periods.", "contents": "Computerization of data from continuous and sequential air monitoring systems. Continuous and sequential area air monitoring systems are more frequently becoming a necessity in both new and existing chemical manufacturing and processing plants. While these systems have most frequently been installed to alert employees to conditions of possible acute overexposure, they generate a wealth of data valuable in documenting employee exposures over extended period of time. Within the Dow Chemical Company, use of a dedicated microcomputer with area air monitoring systems has proven helful in summarizing each days' data and in calculating month-to-date totals for use in employee exposure documentation over long time periods."}
{"id": "PMID:484472", "title": "Formation of carbon monoxide in air compressors.", "content": "Oil-lubricated compressors are said to contaminate the air they compress with carbon monixde from pyrolyzed and oxidized lubricants. Experiments were performed in an instrumented compressor to determine if synthetic oils as lubricants would results in less contamination. The results suggest that for all oils the contamination is actually not great. Published results from low-temperature oxidation of hydrocarbons support this estimate.", "contents": "Formation of carbon monoxide in air compressors. Oil-lubricated compressors are said to contaminate the air they compress with carbon monixde from pyrolyzed and oxidized lubricants. Experiments were performed in an instrumented compressor to determine if synthetic oils as lubricants would results in less contamination. The results suggest that for all oils the contamination is actually not great. Published results from low-temperature oxidation of hydrocarbons support this estimate."}
{"id": "PMID:484473", "title": "AIHA metrication update.", "content": "One of the major advantages of the SI is its precise nomenclature and rules of use. Close compliance with the SI nomenclature and rules of use assures that the SI are universally understood without additional parenthetical explanations. In the english system where symbols, abbreviations and other modifications are continuously changing one can never be sure what CFM, cfm, scf, acf, etc. mean unless the author first defines these abbreviations. More detailed information regarding the use of the SI are contained in the references cited at the end of this article.", "contents": "AIHA metrication update. One of the major advantages of the SI is its precise nomenclature and rules of use. Close compliance with the SI nomenclature and rules of use assures that the SI are universally understood without additional parenthetical explanations. In the english system where symbols, abbreviations and other modifications are continuously changing one can never be sure what CFM, cfm, scf, acf, etc. mean unless the author first defines these abbreviations. More detailed information regarding the use of the SI are contained in the references cited at the end of this article."}
{"id": "PMID:484478", "title": "Computer handling of occupational exposure data.", "content": "A system has been developed for the computer handling of industrial hygiene data in the Michigan Division of Dow Chemical U.S.A. With the implementation of this system, future epidemiological studies might be completed in a few weeks as opposed to months when done menually. The system is capable of providing lists, either current or historical, of the chemicals used in a particular building; and all the available exposure data for specific employees can be obtained throughout their entire periods of employment. The system is also capable of summarizing all available exposure information for a given chemical. The heart of the chemical exposure data system lies in the record content. Each record contains: 1. Chemical identification number; 2. Employee number; 3. Job classification number; 4. Department index number; 5. Data type code; 6. Exposure data; 7. Units code; 8. Date; 9. Building number and 10. Reference. A detailed discussion of each of these parameters is contained in the text.", "contents": "Computer handling of occupational exposure data. A system has been developed for the computer handling of industrial hygiene data in the Michigan Division of Dow Chemical U.S.A. With the implementation of this system, future epidemiological studies might be completed in a few weeks as opposed to months when done menually. The system is capable of providing lists, either current or historical, of the chemicals used in a particular building; and all the available exposure data for specific employees can be obtained throughout their entire periods of employment. The system is also capable of summarizing all available exposure information for a given chemical. The heart of the chemical exposure data system lies in the record content. Each record contains: 1. Chemical identification number; 2. Employee number; 3. Job classification number; 4. Department index number; 5. Data type code; 6. Exposure data; 7. Units code; 8. Date; 9. Building number and 10. Reference. A detailed discussion of each of these parameters is contained in the text."}
{"id": "PMID:484479", "title": "Artificial cutting-fluid smoke generation: effect of pressure.", "content": "Industrial smoke from cutting fluid poses a potential carcinogenic hazard to workers. For toxicity tests, an artificial smoke device needs to be designed. Such a device would be easy to construct if it were known that smoke is a function of heat during cutting processes and not the cutting pressures. Gas chromatograph response was the same for smoke generated with and without pressure. The implication is that smoke generated during a metal cutting process is due to high temperature and is not influenced by the cutting surface pressures.", "contents": "Artificial cutting-fluid smoke generation: effect of pressure. Industrial smoke from cutting fluid poses a potential carcinogenic hazard to workers. For toxicity tests, an artificial smoke device needs to be designed. Such a device would be easy to construct if it were known that smoke is a function of heat during cutting processes and not the cutting pressures. Gas chromatograph response was the same for smoke generated with and without pressure. The implication is that smoke generated during a metal cutting process is due to high temperature and is not influenced by the cutting surface pressures."}
{"id": "PMID:484480", "title": "A method for measuring respiratory volume parameters of large animals during exposure to aerosols.", "content": "The respiratory volume monitor was specifically designed to aid in quantitative control of exposures of beagle dogs to aerosols without adversely affecting the quality of the aerosol. The system monitors and displays tidal volume, total inhaled volume, breath count and elapsed time of exposure. The error in the total inhaled volume measurement does not exceed 10%.", "contents": "A method for measuring respiratory volume parameters of large animals during exposure to aerosols. The respiratory volume monitor was specifically designed to aid in quantitative control of exposures of beagle dogs to aerosols without adversely affecting the quality of the aerosol. The system monitors and displays tidal volume, total inhaled volume, breath count and elapsed time of exposure. The error in the total inhaled volume measurement does not exceed 10%."}
{"id": "PMID:484482", "title": "Personal sampler for NOx.", "content": "A personal sampler system for NOx (NO + NO2) and NO2 has been developed for monitoring workplace air. The NO2 sampler previously reported from this laboratory uses triethanolamine to trap NO2 which diffuses through a tube of appropriate dimensions. The NOx sampler contains the same elements as the NO2 device, but it is also fitted with a chromic acid impregnated disc; this disc converts NO to NO2 which is then trapped by the triethanolamine along with preformed NO2. The trapped NO2 in all cases is determined as nitrite colorimetrically and NO is measured by difference between the NOx and NO2 values. The NOx sampler gives accurate and reproducible results if the chromic acid disc is in place for 24 hours or less; it is necessary, therefore, to insert and remove the disc within reasonably short times before and after sampling. We believe, however, that this operation will not be a serious problem for the user.", "contents": "Personal sampler for NOx. A personal sampler system for NOx (NO + NO2) and NO2 has been developed for monitoring workplace air. The NO2 sampler previously reported from this laboratory uses triethanolamine to trap NO2 which diffuses through a tube of appropriate dimensions. The NOx sampler contains the same elements as the NO2 device, but it is also fitted with a chromic acid impregnated disc; this disc converts NO to NO2 which is then trapped by the triethanolamine along with preformed NO2. The trapped NO2 in all cases is determined as nitrite colorimetrically and NO is measured by difference between the NOx and NO2 values. The NOx sampler gives accurate and reproducible results if the chromic acid disc is in place for 24 hours or less; it is necessary, therefore, to insert and remove the disc within reasonably short times before and after sampling. We believe, however, that this operation will not be a serious problem for the user."}
{"id": "PMID:484483", "title": "Acute toxicity of methyl fluorosulfonate (Magic Methyl).", "content": "Methyl fluorosulfonate (Magic Methyl), an active methylating agent used by research chemists, was studied for acute oral toxicity, acute inhalation toxicity, ocular irritation, and dermal irritation. This compound is very hazardous and may have been responsible for at least one human death. The results of these studies confirmed that Magic Methyl is markedly toxic by all routes studied and particularly by the inhalation route since the LC50 value for rats was found to be between 5 and 6 ppm.", "contents": "Acute toxicity of methyl fluorosulfonate (Magic Methyl). Methyl fluorosulfonate (Magic Methyl), an active methylating agent used by research chemists, was studied for acute oral toxicity, acute inhalation toxicity, ocular irritation, and dermal irritation. This compound is very hazardous and may have been responsible for at least one human death. The results of these studies confirmed that Magic Methyl is markedly toxic by all routes studied and particularly by the inhalation route since the LC50 value for rats was found to be between 5 and 6 ppm."}
{"id": "PMID:484484", "title": "An effective hearing conservation program.", "content": "In a major chemical producing complex, noise problems of all kinds are encountered. One complex of The Dow Chemical Company, the Michigan Division, has approached this problem by setting up a Hearing Conservation Committee consisting of various disciplines: Safety, Industrial Hygiene, Medical, Engineering, and Production. This committee reviews each plant's Hearing Conservation Program bi-annually until all equivalent exposure ratios are less than one. Audiometric data is entered on a computer for rapid evaluation of periodic audiograms.", "contents": "An effective hearing conservation program. In a major chemical producing complex, noise problems of all kinds are encountered. One complex of The Dow Chemical Company, the Michigan Division, has approached this problem by setting up a Hearing Conservation Committee consisting of various disciplines: Safety, Industrial Hygiene, Medical, Engineering, and Production. This committee reviews each plant's Hearing Conservation Program bi-annually until all equivalent exposure ratios are less than one. Audiometric data is entered on a computer for rapid evaluation of periodic audiograms."}
{"id": "PMID:484486", "title": "Investigation of a dual filter sampling method for gaseous and particulate fluoride.", "content": "A two stage filter method designed to separate gaseous and particulate fluoride utilizing an alkaline treated filter to trap gaseous fluoride and post sampling heat treatment of the filters to promote desorption of gaseous fluoride from particulate phase was compared to standard impinger methods for the collection of hydrogen fluoride. Results indicate a high degree of comparability between the two methods. Initial studies involving the recovery of HF from dusted filters by heat treatment suggest recovery rates may vary directly with the amount of HF sampled for a given dust load.", "contents": "Investigation of a dual filter sampling method for gaseous and particulate fluoride. A two stage filter method designed to separate gaseous and particulate fluoride utilizing an alkaline treated filter to trap gaseous fluoride and post sampling heat treatment of the filters to promote desorption of gaseous fluoride from particulate phase was compared to standard impinger methods for the collection of hydrogen fluoride. Results indicate a high degree of comparability between the two methods. Initial studies involving the recovery of HF from dusted filters by heat treatment suggest recovery rates may vary directly with the amount of HF sampled for a given dust load."}
{"id": "PMID:484487", "title": "Pulmonary deposition of aerosols in individual and group caged rats.", "content": "There has long been a need to define differences in the amount of aerosol inhaled by group caged rodents as compared to the theoretically ideal individually caged rodent. Therefore, an experiment was designed and conducted to gain insight into this problem. Three groups of animals were evaluated for the degree of pulmonary deposition following a single six hour exposure to a 3 mu aerosol of dodecyl alcohol. One group contained individually caged rats, while the other two groups contained rats caged either in groups of three or seven. Analyzing lungs and trachea for the dodecyl alcohol indicated 25.6 micrograms/g, 27.1 microbrams/g, and 23.1 micrograms/g for rats caged individually, in threes, or in sevens, respectively. There was no statistically significant difference between the amount of dodecyl alcohol found in group caged rat lungs as compared to individually caged rats. Thus group housing does not appear to reduce the amount of aerosol inhaled by rats during a six hour whole body exposure.", "contents": "Pulmonary deposition of aerosols in individual and group caged rats. There has long been a need to define differences in the amount of aerosol inhaled by group caged rodents as compared to the theoretically ideal individually caged rodent. Therefore, an experiment was designed and conducted to gain insight into this problem. Three groups of animals were evaluated for the degree of pulmonary deposition following a single six hour exposure to a 3 mu aerosol of dodecyl alcohol. One group contained individually caged rats, while the other two groups contained rats caged either in groups of three or seven. Analyzing lungs and trachea for the dodecyl alcohol indicated 25.6 micrograms/g, 27.1 microbrams/g, and 23.1 micrograms/g for rats caged individually, in threes, or in sevens, respectively. There was no statistically significant difference between the amount of dodecyl alcohol found in group caged rat lungs as compared to individually caged rats. Thus group housing does not appear to reduce the amount of aerosol inhaled by rats during a six hour whole body exposure."}
{"id": "PMID:484488", "title": "Experimental analysis of lead-in-air sources in lead-acid battery manufacture.", "content": "Plant-scale experimental sampling programs were carried out to determine the contribution to the lead-in-air exposure from (a) fork-truck transport of pasted plates in racks and (b) manual loading and unloading of plates from racks. Fork-truck transport was found not significant under \"clean\" conditions. Manual loading and unloading was found significant.", "contents": "Experimental analysis of lead-in-air sources in lead-acid battery manufacture. Plant-scale experimental sampling programs were carried out to determine the contribution to the lead-in-air exposure from (a) fork-truck transport of pasted plates in racks and (b) manual loading and unloading of plates from racks. Fork-truck transport was found not significant under \"clean\" conditions. Manual loading and unloading was found significant."}
{"id": "PMID:484489", "title": "Identification of polycyclic aromatic hydrocarbons in carbon black with reference to cancerogenic risk in tire production.", "content": "Carbon blacks used in tire production have been examined for their polycyclic aromatic hydrocarbons (P.A.H.) content. The analytical procedures commonly adopted are discussed. The results obtained show that, A benzene extraction time of 250 hours is necessary to obtain complete extraction, but for practical purposes, an extraction time of 150 hours is sufficient to extract more than 95% of the PAH. It is possible to identify two classes of blacks in regard to extractables. The one class provides extractables in the range of 200--400 micrograms/g; the second class, in the range of 1000--2000 micrograms/g. No P.A.H. were detected by direct injection of carbon black into the mass spectrograph at 200 degrees C, 10(-6) torr. This was probably due to the very strong bonding to the black particles. The P.A.H. contribute a relative constant percentage of the benzene extracts. It is reasonably possible to exclude any carcinogenic risk to personnel, in a working environment, with an airborne carbon black concentration equal to or less than 3.5 mg/m3, due to the very low P.A.H. content of carbon black and to the very strong P.A.H. bonding to the black particles.", "contents": "Identification of polycyclic aromatic hydrocarbons in carbon black with reference to cancerogenic risk in tire production. Carbon blacks used in tire production have been examined for their polycyclic aromatic hydrocarbons (P.A.H.) content. The analytical procedures commonly adopted are discussed. The results obtained show that, A benzene extraction time of 250 hours is necessary to obtain complete extraction, but for practical purposes, an extraction time of 150 hours is sufficient to extract more than 95% of the PAH. It is possible to identify two classes of blacks in regard to extractables. The one class provides extractables in the range of 200--400 micrograms/g; the second class, in the range of 1000--2000 micrograms/g. No P.A.H. were detected by direct injection of carbon black into the mass spectrograph at 200 degrees C, 10(-6) torr. This was probably due to the very strong bonding to the black particles. The P.A.H. contribute a relative constant percentage of the benzene extracts. It is reasonably possible to exclude any carcinogenic risk to personnel, in a working environment, with an airborne carbon black concentration equal to or less than 3.5 mg/m3, due to the very low P.A.H. content of carbon black and to the very strong P.A.H. bonding to the black particles."}
{"id": "PMID:484490", "title": "Cardiac arrhythmias and blood levels associated with inhalation of Halon 1301.", "content": "In this study we determined airborne concentrations of Halon 1301 (CBrF3) and the associated blood levels which produce cardiac arrhythmias in dogs. Beagle dogs were exposed by inhalation to Halon 1301 concentrations ranging from 5 to 20% and, after five minutes of exposure, were given epinephrine by intravenous injection (8--10 micrograms/kg). Electrocardiograms were recorded. Serious cardiac arrhythmias were produced with concentrations of 7.5% or greater. A second group of dogs with cannulas surgically implanted in the common carotid artery and external jugular vein were exposed to 5%, 7.5% and 10% Halon 1301 for 60 minutes. The blood concentration of Halon 1301 increased rapidly during the first five minutes of exposure, plateaued within twenty minutes, and declined rapidly after exposure. The mean blood concentrations at equilibrium were directly proportional to airborne concentrations: at a concentration of 5% in air -- arterial 19.2 micrograms/mL, venous 14.6 micrograms/mL; at 7.5% in air -- arterial 30.6 micrograms/mL, venous 28.4 micrograms/mL; and at 10% in air -- arterial 402 micrograms/mL, venous 32.1 microgram/mL. Since there was no rapid increase in blood fluorocarbon concentration after the first five minutes of exposure, it does not seem likely that risk of cardiac sensitization would increase with increased length of exposure to a given concentration.", "contents": "Cardiac arrhythmias and blood levels associated with inhalation of Halon 1301. In this study we determined airborne concentrations of Halon 1301 (CBrF3) and the associated blood levels which produce cardiac arrhythmias in dogs. Beagle dogs were exposed by inhalation to Halon 1301 concentrations ranging from 5 to 20% and, after five minutes of exposure, were given epinephrine by intravenous injection (8--10 micrograms/kg). Electrocardiograms were recorded. Serious cardiac arrhythmias were produced with concentrations of 7.5% or greater. A second group of dogs with cannulas surgically implanted in the common carotid artery and external jugular vein were exposed to 5%, 7.5% and 10% Halon 1301 for 60 minutes. The blood concentration of Halon 1301 increased rapidly during the first five minutes of exposure, plateaued within twenty minutes, and declined rapidly after exposure. The mean blood concentrations at equilibrium were directly proportional to airborne concentrations: at a concentration of 5% in air -- arterial 19.2 micrograms/mL, venous 14.6 micrograms/mL; at 7.5% in air -- arterial 30.6 micrograms/mL, venous 28.4 micrograms/mL; and at 10% in air -- arterial 402 micrograms/mL, venous 32.1 microgram/mL. Since there was no rapid increase in blood fluorocarbon concentration after the first five minutes of exposure, it does not seem likely that risk of cardiac sensitization would increase with increased length of exposure to a given concentration."}
{"id": "PMID:484494", "title": "R on T or R on P phenomenon? Relation to the genesis of ventricular tachycardia.", "content": "Continuous electrocardiographic monitoring of 225 patients with acute myocardial infarction was performed during the initial 48 hours after admission. Two hundred twelve episodes of ventricular tachycardia occurred in 49 subjects, and 8 patients had primary ventricular fibrillation. Most cases of ventricular tachycardia were associated with late coupling of premature ventricular complexes. Of the 212 instances of ventricular tachycardia, 42 (20 percent) were initiated by a premature complex on the T wave (R on T) (R-R'/Q-T less than 1), and 93 (44 percent) had initiating premature complexes that occurred directly after onset of the sinus P wave (R on P). Of eight episodes of ventricular fibrillation, seven were initiated by a premature ventricular complex and in four of these there was associated R on T phenomenon. The influence of atrial contraction and myocardial stretch on reentry or ectopy is proposed as a possible explanation for the relatively high incidence rate of ventricular tachycardia observed after the onset of the sinus P wave.", "contents": "R on T or R on P phenomenon? Relation to the genesis of ventricular tachycardia. Continuous electrocardiographic monitoring of 225 patients with acute myocardial infarction was performed during the initial 48 hours after admission. Two hundred twelve episodes of ventricular tachycardia occurred in 49 subjects, and 8 patients had primary ventricular fibrillation. Most cases of ventricular tachycardia were associated with late coupling of premature ventricular complexes. Of the 212 instances of ventricular tachycardia, 42 (20 percent) were initiated by a premature complex on the T wave (R on T) (R-R'/Q-T less than 1), and 93 (44 percent) had initiating premature complexes that occurred directly after onset of the sinus P wave (R on P). Of eight episodes of ventricular fibrillation, seven were initiated by a premature ventricular complex and in four of these there was associated R on T phenomenon. The influence of atrial contraction and myocardial stretch on reentry or ectopy is proposed as a possible explanation for the relatively high incidence rate of ventricular tachycardia observed after the onset of the sinus P wave."}
{"id": "PMID:484496", "title": "Quantification of thallium-201 scintigrams in acute myocardial infarction.", "content": "A method has been developed for measurement of myocardial infarct size from thallium-201 scintigrams that depends on computer measurement of levels of radioactivity in the myocardium. In 16 dogs, thallium-201 scintigrams were obtained in the left lateral and left anterior oblique projections 48 hours after ligation of the left anterior descending coronary artery. Scintigraphic results were obtained by two independent observers and were compared with tissue measurements of infarct volume calculated from thallium autoradiograms and nitro-blue tetrazolium (NBT)-stained tissue slices. Infarct volumes derived from tissue measurements were used to develop criteria for the computer scintigraphic technique. There was no significant difference in the scintigraphic measurements made by the two observers. Scintigraphic infarct size in the left lateral and left anterior oblique projections correlated with tissue infarct size with r values of 0.88 and 0.75, respectively, for thallium autoradiography and 0.71 and 0.70, respectively, for NBT tissue staining. The range of infarct volume was 3.3 to 14.8 percent of the left ventricular mass. Results of this study suggest that scintigraphic quantitation of infarct size is feasible in this dog model.", "contents": "Quantification of thallium-201 scintigrams in acute myocardial infarction. A method has been developed for measurement of myocardial infarct size from thallium-201 scintigrams that depends on computer measurement of levels of radioactivity in the myocardium. In 16 dogs, thallium-201 scintigrams were obtained in the left lateral and left anterior oblique projections 48 hours after ligation of the left anterior descending coronary artery. Scintigraphic results were obtained by two independent observers and were compared with tissue measurements of infarct volume calculated from thallium autoradiograms and nitro-blue tetrazolium (NBT)-stained tissue slices. Infarct volumes derived from tissue measurements were used to develop criteria for the computer scintigraphic technique. There was no significant difference in the scintigraphic measurements made by the two observers. Scintigraphic infarct size in the left lateral and left anterior oblique projections correlated with tissue infarct size with r values of 0.88 and 0.75, respectively, for thallium autoradiography and 0.71 and 0.70, respectively, for NBT tissue staining. The range of infarct volume was 3.3 to 14.8 percent of the left ventricular mass. Results of this study suggest that scintigraphic quantitation of infarct size is feasible in this dog model."}
{"id": "PMID:484497", "title": "Critical aortic coarctation: patch aortoplasty in infants less than age 3 months.", "content": "Twenty-three infants less than age 3 months (mean age 31 days) underwent patch aortoplasty for relief of coarctation of the aorta. All had intractable congestive heart failure, despite aggressive medical therapy. Each infant had other cardiac anomalies, including patent ductus arteriosus (83 percent) and ventricular septal defect (74 percent). All patients underwent closure of the ductus arteriosus and patch angioplasty of the aorta to produce a luminal diameter of at least 16 mm. In addition, 9 of the 17 patients (53 percent) with a large shunt ventricular septal defect underwent pulmonary arterial banding. There was one hospital death 42 days after operation secondary to bowel perforation and sepsis. Hospitalization beyond 21 days postoperatively was always due to other unrepaired cardiac lesions. The three late deaths at 3, 9 and 18 months after operation were associated with additional major anomalies. Fourteen patients have had postoperative catheterization. No gradient was found across the site of coarctation repair, but one patient had a gradient between the left carotid and left subclavian arteries. Surgical repair of critical coarctation of the aorta in infants can safely be offered despite the presence of other cardiac anomalies.", "contents": "Critical aortic coarctation: patch aortoplasty in infants less than age 3 months. Twenty-three infants less than age 3 months (mean age 31 days) underwent patch aortoplasty for relief of coarctation of the aorta. All had intractable congestive heart failure, despite aggressive medical therapy. Each infant had other cardiac anomalies, including patent ductus arteriosus (83 percent) and ventricular septal defect (74 percent). All patients underwent closure of the ductus arteriosus and patch angioplasty of the aorta to produce a luminal diameter of at least 16 mm. In addition, 9 of the 17 patients (53 percent) with a large shunt ventricular septal defect underwent pulmonary arterial banding. There was one hospital death 42 days after operation secondary to bowel perforation and sepsis. Hospitalization beyond 21 days postoperatively was always due to other unrepaired cardiac lesions. The three late deaths at 3, 9 and 18 months after operation were associated with additional major anomalies. Fourteen patients have had postoperative catheterization. No gradient was found across the site of coarctation repair, but one patient had a gradient between the left carotid and left subclavian arteries. Surgical repair of critical coarctation of the aorta in infants can safely be offered despite the presence of other cardiac anomalies."}
{"id": "PMID:484499", "title": "Blood pressure, heart rate, pressure-rate product and electrocardiographic changes in healthy children during treadmill exercise.", "content": "Heart rate, systolic and diastolic blood pressure, rate-pressure product and electrocardiographic changes were measured in 288 healthy children during treadmill exercise. A constant speed (3.5 miles/hour) protocol was used to facilitate the measurements of physiologic data during exercise. Because responses to exercise vary with size, the children were separated into four groups according to body surface area. Comparisons were also made between male and female and between black and white children. The values for heart rate and systolic pressure showed excellent reproducibility in repeated tests. These data establish for clinical use reference values for children during treadmill exercise.", "contents": "Blood pressure, heart rate, pressure-rate product and electrocardiographic changes in healthy children during treadmill exercise. Heart rate, systolic and diastolic blood pressure, rate-pressure product and electrocardiographic changes were measured in 288 healthy children during treadmill exercise. A constant speed (3.5 miles/hour) protocol was used to facilitate the measurements of physiologic data during exercise. Because responses to exercise vary with size, the children were separated into four groups according to body surface area. Comparisons were also made between male and female and between black and white children. The values for heart rate and systolic pressure showed excellent reproducibility in repeated tests. These data establish for clinical use reference values for children during treadmill exercise."}
{"id": "PMID:484501", "title": "Effects of oral quinidine on left ventricular performance in normal subjects and patients with congestive cardiomyopathy.", "content": "To evaluate the effects of oral quinidine therapy on left ventricular performance, 10 normal subjects and 8 patients with cardiomyopathy were studied with echocardiography at rest, after intravenous injection of atropine and during infusion with phenylephrine. The echocardiographic studies were performed during oral quinidine therapy and during placebo administration. In the normal subjects heart rate was significantly faster with quinidine than with placebo (74 +/- 8 (standard deviation) versus 68 +/- 9 beats/min, P less than 0.01), but there was no significant change in blood pressure or left ventricular size and performance. After administration of atropine, heart rate was identical with and without quinidine but the mean normalized velocity of left ventricular dimension shortening was significantly less with quinidine than with placebo (1.28 +/- 0.19 versus 1.44 +/- 0.21, P less than 0.01). During acute pressure loading with phenylephrine there was no difference in left ventricular size or performance during quinidine therapy. In the patients with cardiomyopathy, no significant differences in left ventricular function were detected with this protocol during quinidine therapy. It is concluded that oral quinidine therapy appears to have little adverse effect on left ventricular performance at rest or during acute pressure loading.", "contents": "Effects of oral quinidine on left ventricular performance in normal subjects and patients with congestive cardiomyopathy. To evaluate the effects of oral quinidine therapy on left ventricular performance, 10 normal subjects and 8 patients with cardiomyopathy were studied with echocardiography at rest, after intravenous injection of atropine and during infusion with phenylephrine. The echocardiographic studies were performed during oral quinidine therapy and during placebo administration. In the normal subjects heart rate was significantly faster with quinidine than with placebo (74 +/- 8 (standard deviation) versus 68 +/- 9 beats/min, P less than 0.01), but there was no significant change in blood pressure or left ventricular size and performance. After administration of atropine, heart rate was identical with and without quinidine but the mean normalized velocity of left ventricular dimension shortening was significantly less with quinidine than with placebo (1.28 +/- 0.19 versus 1.44 +/- 0.21, P less than 0.01). During acute pressure loading with phenylephrine there was no difference in left ventricular size or performance during quinidine therapy. In the patients with cardiomyopathy, no significant differences in left ventricular function were detected with this protocol during quinidine therapy. It is concluded that oral quinidine therapy appears to have little adverse effect on left ventricular performance at rest or during acute pressure loading."}
{"id": "PMID:484503", "title": "Serial echocardiographic appearance of healing bacterial vegetations.", "content": "Serial M mode and cross-sectional echocardiograms were obtained from six patients who had been treated with antibiotic drugs for infectious endocarditis. Three to six M mode echocardiograms and one to six cross-sectional echocardiograms were obtained from each patient over a follow-up period averaging 50 weeks (range 10 to 108 weeks). On echocardiography, vegetations were observed to have become smaller and more echo-reflective with healing. A dramatic change was seen in two patients after peripheral embolization. M mode echocardiography was particularly helpful in determining the quality of echo reflection by vegetations; cross-sectional echocardiography was more helpful in judging the size and shape of a vegetation. Echocardiography is ideally suited for the serial visualization of healing vegetations in patients who do not require early valve replacement. It may prove helpful to examine serially valve vegetations with both M mode and cross-sectional echocardiography when following up patients with infectious endocarditis treated with antibiotic agents.", "contents": "Serial echocardiographic appearance of healing bacterial vegetations. Serial M mode and cross-sectional echocardiograms were obtained from six patients who had been treated with antibiotic drugs for infectious endocarditis. Three to six M mode echocardiograms and one to six cross-sectional echocardiograms were obtained from each patient over a follow-up period averaging 50 weeks (range 10 to 108 weeks). On echocardiography, vegetations were observed to have become smaller and more echo-reflective with healing. A dramatic change was seen in two patients after peripheral embolization. M mode echocardiography was particularly helpful in determining the quality of echo reflection by vegetations; cross-sectional echocardiography was more helpful in judging the size and shape of a vegetation. Echocardiography is ideally suited for the serial visualization of healing vegetations in patients who do not require early valve replacement. It may prove helpful to examine serially valve vegetations with both M mode and cross-sectional echocardiography when following up patients with infectious endocarditis treated with antibiotic agents."}
{"id": "PMID:484504", "title": "Bilateral cardiac myxomas and peripheral myxomas in a patient with recent myocardial infarction.", "content": "Coronary arteriography, performed for recent anterior wall myocardial infarction in a 29 year old man, showed multiple aneurysms of the distal branches of the left coronary artery without evidence of obstructions in the major branches, and two clusters of abnormal vessels, supplying tumors in the right ventricle and the left atrium. At operation and histologic examination both tumors appeared to be myxomas. In addition, two myxomas, located elswhere in the body, could be proven histologically.", "contents": "Bilateral cardiac myxomas and peripheral myxomas in a patient with recent myocardial infarction. Coronary arteriography, performed for recent anterior wall myocardial infarction in a 29 year old man, showed multiple aneurysms of the distal branches of the left coronary artery without evidence of obstructions in the major branches, and two clusters of abnormal vessels, supplying tumors in the right ventricle and the left atrium. At operation and histologic examination both tumors appeared to be myxomas. In addition, two myxomas, located elswhere in the body, could be proven histologically."}
{"id": "PMID:484509", "title": "Ultrastructural organization of the hamster renal pelvis.", "content": "The renal pelvis of the hamster has been studied by light microscopy (epoxy resin sections), transmission electron microscopy, and morphometric analysis of electron micrographs. Three morphologically distinct epithelia line the pelvis, and each covers a different zone of the kidney. A thin epithelium covering the outer medulla (OM) consists of two cell types: (1) granular cells are most numerous and have apically positioned granules which stain intensely with toluidine blue, are membrane-bound, and contain a fine particulate matter that stains light grey to black in electron micrographs. (2) Basal cells do not have granules, are confined to the basal lamina region, and do not reach the mucosal epithelial surface. The inner medulla (IM) is covered by a pelvic epithelium morphologically similar to collecting duct epithelium of IM. Some cells in this portion of the pelvic epithelium (IM) stain intensely dark with toluidine blue, osmium tetroxide, lead, and uranyl acetate. Transitional epithelium, which separates cortex (C) from pelvic urine, has an asymmetric luminal plasma membrane and discoid vesicles, each of which is similar to those previously observed in mammalian ureter and urinary bladder epithelia. Based on morphological comparisons with other epithelia, the IM and OM pelvic epithelia would appear permeable to solutes and/or water, while the transitional epithelium covering the C appears relatively impermeable. It would also appear that the exchange of solutes and water between pelvic urine and OM would involve capillaries, primarily, since morphometric analysis showed that both fenestrated and continuous capillaries of the OM were extremely abundant (greater than 60% of OM pelvic surface area) just under the thin pelvic epithelium.", "contents": "Ultrastructural organization of the hamster renal pelvis. The renal pelvis of the hamster has been studied by light microscopy (epoxy resin sections), transmission electron microscopy, and morphometric analysis of electron micrographs. Three morphologically distinct epithelia line the pelvis, and each covers a different zone of the kidney. A thin epithelium covering the outer medulla (OM) consists of two cell types: (1) granular cells are most numerous and have apically positioned granules which stain intensely with toluidine blue, are membrane-bound, and contain a fine particulate matter that stains light grey to black in electron micrographs. (2) Basal cells do not have granules, are confined to the basal lamina region, and do not reach the mucosal epithelial surface. The inner medulla (IM) is covered by a pelvic epithelium morphologically similar to collecting duct epithelium of IM. Some cells in this portion of the pelvic epithelium (IM) stain intensely dark with toluidine blue, osmium tetroxide, lead, and uranyl acetate. Transitional epithelium, which separates cortex (C) from pelvic urine, has an asymmetric luminal plasma membrane and discoid vesicles, each of which is similar to those previously observed in mammalian ureter and urinary bladder epithelia. Based on morphological comparisons with other epithelia, the IM and OM pelvic epithelia would appear permeable to solutes and/or water, while the transitional epithelium covering the C appears relatively impermeable. It would also appear that the exchange of solutes and water between pelvic urine and OM would involve capillaries, primarily, since morphometric analysis showed that both fenestrated and continuous capillaries of the OM were extremely abundant (greater than 60% of OM pelvic surface area) just under the thin pelvic epithelium."}
{"id": "PMID:484510", "title": "Cephalic neurulation and optic vesicle formation in the early mouse embryo.", "content": "The overall pattern of cephalic neurulation and the concomitant early development of the optic vesicles in mouse embryos were examined by scanning electron microscopy. Paraffin-sectioned specimens were also examined. The overall pattern of closure of the cephalic neural folds accords well with earlier observations of this process. The earliest indication of optic placode formation was seen in histological sections of embryos at the 4-somite stage, while optic pit formation was first observed at the 5- to 6-somite stage. The upper halves of the optic vesicles were formed in 10- to 15-somite embryos by the fusion of the neural folds at the junction between the mesencephalon and prosencephalon, while closure of the lower halves was associated with the closure of the rostral neuropore, and was usually completed by about the 20-somite stage. By the 25- to 30-somite stage, a rapid increase in the volume of the forebrain was observed, so that the optic vesicles were displaced laterally. An overall increase in the volume of the optic vesicles and decrease in the diameter of the optic stalks were also observed at this time. This account of cephalic neurulation and optic organogenesis provides useful baseline data relevant to the study of the normal early development of the mouse. A comparison is made between similar events in the rat, the hamster, and the human embryo.", "contents": "Cephalic neurulation and optic vesicle formation in the early mouse embryo. The overall pattern of cephalic neurulation and the concomitant early development of the optic vesicles in mouse embryos were examined by scanning electron microscopy. Paraffin-sectioned specimens were also examined. The overall pattern of closure of the cephalic neural folds accords well with earlier observations of this process. The earliest indication of optic placode formation was seen in histological sections of embryos at the 4-somite stage, while optic pit formation was first observed at the 5- to 6-somite stage. The upper halves of the optic vesicles were formed in 10- to 15-somite embryos by the fusion of the neural folds at the junction between the mesencephalon and prosencephalon, while closure of the lower halves was associated with the closure of the rostral neuropore, and was usually completed by about the 20-somite stage. By the 25- to 30-somite stage, a rapid increase in the volume of the forebrain was observed, so that the optic vesicles were displaced laterally. An overall increase in the volume of the optic vesicles and decrease in the diameter of the optic stalks were also observed at this time. This account of cephalic neurulation and optic organogenesis provides useful baseline data relevant to the study of the normal early development of the mouse. A comparison is made between similar events in the rat, the hamster, and the human embryo."}
{"id": "PMID:484511", "title": "Observations on closure of the neuropores in the chick embryo.", "content": "Neuropore closure was studied in chick embryos by light and electron microscopy. Surface ectoderm reflects over the crests of the neural folds at all craniocaudal levels, merging with the neural ectoderm lining the neural groove. Apices of surface ectodermal cells have an essentially identical morphology prior to approximation of folds, both within the presumptive fusion sites and more laterally. Cells of these areas have slightly convex profiles exhibiting few cellular protrusions. Each neural fold contains a superficial half, composed of neural ectoderm covered by surface ectoderm, and a deep half consisting entirely of neural ectoderm. Initial contact between folds usually occurs near the junction between these halves in cranial regions, but is restricted primarily to surface ectoderm at caudal levels. Subsequent fusion of folds at all levels involves both ectodermal layers. Cellular protrusions and small, morphologically unspecialized intercellular junctions often interconnect cells of apposed folds in areas undergoing fusion. The anterior neuropore closes at stages 10-11, but fusion of folds in this region is not completed until stages 13-14. Fusion occurs dorsoventrally in this area and is more advanced internally than externally. Numerous pleomorphic inclusions and a few apparently necrotic cells are present in areas bordering the anterior neuropore. The posterior neuropore closes at stages 12-13 and fusion is completed in this region during stages 13-14. The caudal end of the posterior neuropore closes dorsal to the developing tail bud. Several morphological features of this closure may at least partially account for the high susceptibility to myeloschisis localized specifically at caudal spinal cord levels.", "contents": "Observations on closure of the neuropores in the chick embryo. Neuropore closure was studied in chick embryos by light and electron microscopy. Surface ectoderm reflects over the crests of the neural folds at all craniocaudal levels, merging with the neural ectoderm lining the neural groove. Apices of surface ectodermal cells have an essentially identical morphology prior to approximation of folds, both within the presumptive fusion sites and more laterally. Cells of these areas have slightly convex profiles exhibiting few cellular protrusions. Each neural fold contains a superficial half, composed of neural ectoderm covered by surface ectoderm, and a deep half consisting entirely of neural ectoderm. Initial contact between folds usually occurs near the junction between these halves in cranial regions, but is restricted primarily to surface ectoderm at caudal levels. Subsequent fusion of folds at all levels involves both ectodermal layers. Cellular protrusions and small, morphologically unspecialized intercellular junctions often interconnect cells of apposed folds in areas undergoing fusion. The anterior neuropore closes at stages 10-11, but fusion of folds in this region is not completed until stages 13-14. Fusion occurs dorsoventrally in this area and is more advanced internally than externally. Numerous pleomorphic inclusions and a few apparently necrotic cells are present in areas bordering the anterior neuropore. The posterior neuropore closes at stages 12-13 and fusion is completed in this region during stages 13-14. The caudal end of the posterior neuropore closes dorsal to the developing tail bud. Several morphological features of this closure may at least partially account for the high susceptibility to myeloschisis localized specifically at caudal spinal cord levels."}
{"id": "PMID:484518", "title": "Lactose intolerance in the Lebanese population and in \"Mediterranean lymphoma\".", "content": "Lactose tolerance tests were performed on 75 healthy Lebanese volunteers, 12 patients with \"Mediterranean lymphoma\" and 15 American and West European Caucasians. Small intestinal biopsies were done on 10 intolerant and five tolerant subjects for histological evaluation and lactase assay. Lactose malabsorption was present in 78% of the Lebanese subjects, in all patients with Mediterranean lymphoma and in five of the 15 Caucasians. Two of the five intolerant Caucasians had giardiasis. There was no difference in the prevalence rate among the various Lebanese groups nor among males and females. Symptoms occurred in 91% of the 58 intolerant Lebanese subjects: diarrhea in 71%, abdominal distension in 67%, and cramps in 48%. The increased prevalence of lactose intolerance with Mediterranean lymphoma is probably secondary to the pathological changes in the intestinal mucosa and protein depletion.", "contents": "Lactose intolerance in the Lebanese population and in \"Mediterranean lymphoma\". Lactose tolerance tests were performed on 75 healthy Lebanese volunteers, 12 patients with \"Mediterranean lymphoma\" and 15 American and West European Caucasians. Small intestinal biopsies were done on 10 intolerant and five tolerant subjects for histological evaluation and lactase assay. Lactose malabsorption was present in 78% of the Lebanese subjects, in all patients with Mediterranean lymphoma and in five of the 15 Caucasians. Two of the five intolerant Caucasians had giardiasis. There was no difference in the prevalence rate among the various Lebanese groups nor among males and females. Symptoms occurred in 91% of the 58 intolerant Lebanese subjects: diarrhea in 71%, abdominal distension in 67%, and cramps in 48%. The increased prevalence of lactose intolerance with Mediterranean lymphoma is probably secondary to the pathological changes in the intestinal mucosa and protein depletion."}
{"id": "PMID:484519", "title": "An examination of factors believed to be associated with infantile obesity.", "content": "Forty-two normal (25th to 75th percentiles of weight for age) and 47 \"obese\" (above the 90th percentile) infants, 4 to 9 months of age, were compared with regard to energy and macronutrient intake, breast-feeding history, age of introduction of solids, and maternal reliance on external feeding cues. The study failed to reveal characteristic group differences. The current stereotype of the obese infant as one who is overfed, is given solids very young, is not breast fed, and whose mother is not responsive to his satiety signals is challenged. As a result, the nature of current group counseling for the prevention of infantile obesity is questioned.", "contents": "An examination of factors believed to be associated with infantile obesity. Forty-two normal (25th to 75th percentiles of weight for age) and 47 \"obese\" (above the 90th percentile) infants, 4 to 9 months of age, were compared with regard to energy and macronutrient intake, breast-feeding history, age of introduction of solids, and maternal reliance on external feeding cues. The study failed to reveal characteristic group differences. The current stereotype of the obese infant as one who is overfed, is given solids very young, is not breast fed, and whose mother is not responsive to his satiety signals is challenged. As a result, the nature of current group counseling for the prevention of infantile obesity is questioned."}
{"id": "PMID:484520", "title": "Effects of behavioral and pharmacological weight loss programs on nutrient intake.", "content": "Behavior therapy is a widely used method for short-term weight control. Formal nutrition counseling is not included in many behavioral programs. To determine what types of diets were self-selected by patients and to see how they compared with individuals participating in a relaxation group with anorectic medication, the eating diaries compiled by participants in such a program were analyzed for 20 different nutrients. In general, pretreatment intakes were nutritious and typical for Americans. Both the behavioral and a relaxation-medication treatment produced weight loss and reduction in calorie intake. Both treatments were associated with the consumption of a fairly nutritious diet not much different from the pretreatment intake except for a reduction in the amount eaten.", "contents": "Effects of behavioral and pharmacological weight loss programs on nutrient intake. Behavior therapy is a widely used method for short-term weight control. Formal nutrition counseling is not included in many behavioral programs. To determine what types of diets were self-selected by patients and to see how they compared with individuals participating in a relaxation group with anorectic medication, the eating diaries compiled by participants in such a program were analyzed for 20 different nutrients. In general, pretreatment intakes were nutritious and typical for Americans. Both the behavioral and a relaxation-medication treatment produced weight loss and reduction in calorie intake. Both treatments were associated with the consumption of a fairly nutritious diet not much different from the pretreatment intake except for a reduction in the amount eaten."}
{"id": "PMID:484521", "title": "Comparative study of endogenous fecal fatty acids in germ-free and conventional rats.", "content": "The effects of gastrointestinal flora on the quantitative and qualitative aspects of \"endogenous\" fecal fatty acid excretion were studied in the rat using germ-free (GF) and conventional (CVL) animals, lipid-free diet and open-tubular gas-liquid chromatography. Gastrointestinal flora slightly increased endogenous excretion of fatty acids. Analysis of fatty acids from different fecal lipid fractions showed that the presence of a microflora in the gastrointestinal tract extensively modified the qualitative aspects of endogenous fecal fatty acid spectra. In total endogenous fecal lipids, concentrations of mono and polyunsaturated fatty acids (from C16 to C22) were 3.5 times higher in GF rats, while concentrations of even-numbered and saturated fatty acids were 1.5 times higher in CVL rats. Odd-numbered fatty acids (from C11:10 to C2:0) represented 6.5% of total endogenous fecal fatty acids in CVL rats vensus 1.5% in GF rats. Branched-chain fatty acid (even-and-odd-numbered, iso and anteiso) concentrations were 12.9% in CVL rats versus 2.0% in GF rats. Results clearly showed that the gastrointestinal flora was the main source of odd-numbered fatty acids and branched-chain fatty acids in endogenous fecal lipids. However, a secondary source may also exist since these fatty acids represented 3.5% of the total endogenous fatty acids in GF rats.", "contents": "Comparative study of endogenous fecal fatty acids in germ-free and conventional rats. The effects of gastrointestinal flora on the quantitative and qualitative aspects of \"endogenous\" fecal fatty acid excretion were studied in the rat using germ-free (GF) and conventional (CVL) animals, lipid-free diet and open-tubular gas-liquid chromatography. Gastrointestinal flora slightly increased endogenous excretion of fatty acids. Analysis of fatty acids from different fecal lipid fractions showed that the presence of a microflora in the gastrointestinal tract extensively modified the qualitative aspects of endogenous fecal fatty acid spectra. In total endogenous fecal lipids, concentrations of mono and polyunsaturated fatty acids (from C16 to C22) were 3.5 times higher in GF rats, while concentrations of even-numbered and saturated fatty acids were 1.5 times higher in CVL rats. Odd-numbered fatty acids (from C11:10 to C2:0) represented 6.5% of total endogenous fecal fatty acids in CVL rats vensus 1.5% in GF rats. Branched-chain fatty acid (even-and-odd-numbered, iso and anteiso) concentrations were 12.9% in CVL rats versus 2.0% in GF rats. Results clearly showed that the gastrointestinal flora was the main source of odd-numbered fatty acids and branched-chain fatty acids in endogenous fecal lipids. However, a secondary source may also exist since these fatty acids represented 3.5% of the total endogenous fatty acids in GF rats."}
{"id": "PMID:484522", "title": "Clinical results of a cross-over treatment with pyridoxine and placebo of the carpal tunnel syndrome.", "content": "Clinical evaluation was made of cross-over treatments by pyridoxine and a placebo of patient 22 having the carpal tunnel syndrome. Extraordinary monitoring of the specific activities of the erythrocyte glutamic oxaloacetic transaminase proved a severe vitamin B6 deficiency, which was partially corrected by the Recommended Dietary Allowance of 2 mg, and completely corrected by 100 mg. The severity of the syndrome diminished on the Recommended Dietary Allowances and the patient was asymptomatic at the higher dosage. On placebo, both the vitamin B6 deficiency and syndrome reappeared. Retreatment with 100 mg again corrected both the deficiency and syndrome. Measurements (total n = 19) of flexion of proximal interphalangeal joints of the index fingers by a goniometer, and of pinch by the Preston gauge revealed objective normalization. Scores of 17 symptoms revealed reductions at both the 2- (P less than 0.01) and 100-mg (P less than 0.001) dosages. Conduction through the carpal tunnels had improved by electromyography. These and previous data on a total of 22 patients showed the concomitant presence of a deficiency of vitamin B6 and the carpal tunnel syndrome; a causal relationship is apparent.", "contents": "Clinical results of a cross-over treatment with pyridoxine and placebo of the carpal tunnel syndrome. Clinical evaluation was made of cross-over treatments by pyridoxine and a placebo of patient 22 having the carpal tunnel syndrome. Extraordinary monitoring of the specific activities of the erythrocyte glutamic oxaloacetic transaminase proved a severe vitamin B6 deficiency, which was partially corrected by the Recommended Dietary Allowance of 2 mg, and completely corrected by 100 mg. The severity of the syndrome diminished on the Recommended Dietary Allowances and the patient was asymptomatic at the higher dosage. On placebo, both the vitamin B6 deficiency and syndrome reappeared. Retreatment with 100 mg again corrected both the deficiency and syndrome. Measurements (total n = 19) of flexion of proximal interphalangeal joints of the index fingers by a goniometer, and of pinch by the Preston gauge revealed objective normalization. Scores of 17 symptoms revealed reductions at both the 2- (P less than 0.01) and 100-mg (P less than 0.001) dosages. Conduction through the carpal tunnels had improved by electromyography. These and previous data on a total of 22 patients showed the concomitant presence of a deficiency of vitamin B6 and the carpal tunnel syndrome; a causal relationship is apparent."}
{"id": "PMID:484523", "title": "Riboflavin nutritional status and absorption in oral contraceptive users and nonusers.", "content": "Riboflavin nutritional status, based on erythrocyte glutathione reductase (EGR) determinations, and the absorption of the vitamin after 10-mg oral doses were assessed in a group of oral contraceptive users (group OC, n = 18) and in a group of nonusers (group NOC, n = 17). Before administration of test doses of riboflavin, mean EGR levels of 4.06 (group OC) and 4.44 (group NOC) mumoles glutathione reduced per minute per gram of hemoglobin were not significantly different. After oral administration of riboflavin, a significant increase in EGR levels was observed within each group although no significant differences were detected between groups. Mean EGR levels continued to be slightly lower for group OC compared to group NOC. Oral absorption of riboflavin based on urinary excretion results was not significantly different between the groups. These findings indicate OC use does not significantly affect riboflavin nutritional status in a subject population having adequate nutrition of moderate to high socioeconomic status. Significant reductions in EGR levels which have been previously reported for OC users appear to be associated with a low socioeconomic status.", "contents": "Riboflavin nutritional status and absorption in oral contraceptive users and nonusers. Riboflavin nutritional status, based on erythrocyte glutathione reductase (EGR) determinations, and the absorption of the vitamin after 10-mg oral doses were assessed in a group of oral contraceptive users (group OC, n = 18) and in a group of nonusers (group NOC, n = 17). Before administration of test doses of riboflavin, mean EGR levels of 4.06 (group OC) and 4.44 (group NOC) mumoles glutathione reduced per minute per gram of hemoglobin were not significantly different. After oral administration of riboflavin, a significant increase in EGR levels was observed within each group although no significant differences were detected between groups. Mean EGR levels continued to be slightly lower for group OC compared to group NOC. Oral absorption of riboflavin based on urinary excretion results was not significantly different between the groups. These findings indicate OC use does not significantly affect riboflavin nutritional status in a subject population having adequate nutrition of moderate to high socioeconomic status. Significant reductions in EGR levels which have been previously reported for OC users appear to be associated with a low socioeconomic status."}
{"id": "PMID:484524", "title": "Plasma 1,25-(OH)2-vitamin D concentrations and net intestinal calcium, phosphate, and magnesium absorption in humans.", "content": "We evaluated the relationship between plasma concentrations of the renal hormone 1,25-(OH)2-vitamin D and net intestinal absorption of Ca, PO4, and Mg in vitamin D-replete patients eating similar diets, who had undetectable, normal or elevated plasma 1,25-(OH)2-D levels, Net intestinal Ca absorption was positively correlated to plasma 1,25-(OH)2-D concentrations: percentage dietary Ca absorbed = 10 + 0.17 x plasma total 1,25-(OH)2-3, pmole/liter, r = + 0.58; P less than 0.001. By contrast, there was no significant correlation between PO4 or Mg absorption and plasma 1,25-(OH)2-D concentrations. Moreover, significant quantities of PO4 and Mg were absorbed in the absence of detectable plasma 1,25-(OH)2-D. We conclude that net intestinal Ca absorption is critically dependent upon the availability of the renal hormone 1,25-(OH)2-D in vitamin D-replete humans when dietary Ca intake is normal. By contrast, other factors must play a dominant role in regulating net intestinal PO4 and Mg absorption.", "contents": "Plasma 1,25-(OH)2-vitamin D concentrations and net intestinal calcium, phosphate, and magnesium absorption in humans. We evaluated the relationship between plasma concentrations of the renal hormone 1,25-(OH)2-vitamin D and net intestinal absorption of Ca, PO4, and Mg in vitamin D-replete patients eating similar diets, who had undetectable, normal or elevated plasma 1,25-(OH)2-D levels, Net intestinal Ca absorption was positively correlated to plasma 1,25-(OH)2-D concentrations: percentage dietary Ca absorbed = 10 + 0.17 x plasma total 1,25-(OH)2-3, pmole/liter, r = + 0.58; P less than 0.001. By contrast, there was no significant correlation between PO4 or Mg absorption and plasma 1,25-(OH)2-D concentrations. Moreover, significant quantities of PO4 and Mg were absorbed in the absence of detectable plasma 1,25-(OH)2-D. We conclude that net intestinal Ca absorption is critically dependent upon the availability of the renal hormone 1,25-(OH)2-D in vitamin D-replete humans when dietary Ca intake is normal. By contrast, other factors must play a dominant role in regulating net intestinal PO4 and Mg absorption."}
{"id": "PMID:484525", "title": "Serum alpha-tocopherol, lipids, potassium, and creatine phosphokinase in normal and malabsorption patients.", "content": "Serum alpha-tocopherol, lipids, potassium, and creatine phosphokinase levels were measured in 20 adult male control patients and eight malabsorption patients. The malabsorption group had significantly lower serum alpha-tocopherol levels than the control group. This change was independent of serum total lipid levels that were not significantly different among the two groups. Serum potassium and creatine phosphokinase levels that are normally used to assess muscle pathology in man did not correlate with serum alpha-tocopherol levels in either the control of the malabsorption groups. Body mass indices that are directly related to adiposity of the individuals were calculated. Among the control patients, there was a significant increase in serum alpha-tocopherol and serum total lipids with increase in body mass index. Similar correlations did not exist in the malabsorption group. In the latter group serum alpha-tocopherol levels may have reached low enough levels to be independent of factors such as adiposity and serum total lipids.", "contents": "Serum alpha-tocopherol, lipids, potassium, and creatine phosphokinase in normal and malabsorption patients. Serum alpha-tocopherol, lipids, potassium, and creatine phosphokinase levels were measured in 20 adult male control patients and eight malabsorption patients. The malabsorption group had significantly lower serum alpha-tocopherol levels than the control group. This change was independent of serum total lipid levels that were not significantly different among the two groups. Serum potassium and creatine phosphokinase levels that are normally used to assess muscle pathology in man did not correlate with serum alpha-tocopherol levels in either the control of the malabsorption groups. Body mass indices that are directly related to adiposity of the individuals were calculated. Among the control patients, there was a significant increase in serum alpha-tocopherol and serum total lipids with increase in body mass index. Similar correlations did not exist in the malabsorption group. In the latter group serum alpha-tocopherol levels may have reached low enough levels to be independent of factors such as adiposity and serum total lipids."}
{"id": "PMID:484526", "title": "Utilization of swine to study the risk factor of an elevated salt diet on blood pressure.", "content": "Two homogeneous groups of castrated swine were fed identical diets except that one contained an increased sodium chloride content. The diets were initiated at weaning age and the blood pressure of the pig was followed for 8 months. Blood pressures were measured with an automatic blood pressure recorder for a 1-week period every 4th week. At the end of 8 months, the group on the high salt diet had blood pressure elevated above that of the group on the low salt diet.", "contents": "Utilization of swine to study the risk factor of an elevated salt diet on blood pressure. Two homogeneous groups of castrated swine were fed identical diets except that one contained an increased sodium chloride content. The diets were initiated at weaning age and the blood pressure of the pig was followed for 8 months. Blood pressures were measured with an automatic blood pressure recorder for a 1-week period every 4th week. At the end of 8 months, the group on the high salt diet had blood pressure elevated above that of the group on the low salt diet."}
{"id": "PMID:484529", "title": "Assessing iron status of a population.", "content": "Reliable methods for assessing the iron status of a population are essential for developing effective public health measures to combat iron deficiency. The hemoglobin concentration, transferrin saturation, free erythrocyte protoporphyrin, and serum ferritin are all useful but they vary widely in their specificity and sensitivity for detecting iron deficiency. In applying these laboratory parameters, the usual approach in nutritional surveys is to determine the percentage of values outside the normal range. As an alternative, a model is presented here that uses these measurements to estimate the distribution of iron stores in a population. This approach may be particularly useful for evaluating the effectiveness of iron supplementation and fortification programs.", "contents": "Assessing iron status of a population. Reliable methods for assessing the iron status of a population are essential for developing effective public health measures to combat iron deficiency. The hemoglobin concentration, transferrin saturation, free erythrocyte protoporphyrin, and serum ferritin are all useful but they vary widely in their specificity and sensitivity for detecting iron deficiency. In applying these laboratory parameters, the usual approach in nutritional surveys is to determine the percentage of values outside the normal range. As an alternative, a model is presented here that uses these measurements to estimate the distribution of iron stores in a population. This approach may be particularly useful for evaluating the effectiveness of iron supplementation and fortification programs."}
{"id": "PMID:484530", "title": "The association of obesity with socioeconomic factors in Missouri.", "content": "The prevalence of obesity and underweight was estimated for residents of Missouri more than 9 years old on the basis of age, sex, household income, educational attainment, and population density from the results of a survey conducted in 1973. The incidence of overweight was greatest among children 10 to 16 years old and the least for adults 17 to 35 years old. Four percent of the girls 10 to 16 were greater than 159% of average weight for height. Among women greater than 59 years old the incidence of overweight and underweight were high. The proportion of adult women who were overweight as defined by average weight for height and body mass index (W/H2) was inversely related to household income and education. The same was true for adult men in relation to income but the reverse results were observed for education. A larger proportion of urban men were overweight as defined by body mass index compared to rural men. The incidence of obesity among males of all ages and females greater than 59 years old appeared to be related to residence in specific types of communities.", "contents": "The association of obesity with socioeconomic factors in Missouri. The prevalence of obesity and underweight was estimated for residents of Missouri more than 9 years old on the basis of age, sex, household income, educational attainment, and population density from the results of a survey conducted in 1973. The incidence of overweight was greatest among children 10 to 16 years old and the least for adults 17 to 35 years old. Four percent of the girls 10 to 16 were greater than 159% of average weight for height. Among women greater than 59 years old the incidence of overweight and underweight were high. The proportion of adult women who were overweight as defined by average weight for height and body mass index (W/H2) was inversely related to household income and education. The same was true for adult men in relation to income but the reverse results were observed for education. A larger proportion of urban men were overweight as defined by body mass index compared to rural men. The incidence of obesity among males of all ages and females greater than 59 years old appeared to be related to residence in specific types of communities."}
{"id": "PMID:484531", "title": "Geophagia in rural Mississippi: environmental and cultural contexts and nutritional implications.", "content": "We investigated geophagia in the black population of rural Holmes County, Mississippi. Twenty-five sources of geophagical clays were located and most of the sources are associated with rural settlements throughout the county. Clays are taken from subsurface soil horizons, and all but one of the sources come from the upland portion of the county. Geophagia occurs among 57% of women and 16% of children of both sexes, but it is not found among adult males or adolescents. Average daily comsumption of clay is 50 g. Our data indicate geophagia is not correlated with hunger, anemia, or helminthic problems, but it may contribute to the common problem of hypertension. Geophagia has been suggested as one of the factors leading to hyperkalemia, but our data do not support this notion.", "contents": "Geophagia in rural Mississippi: environmental and cultural contexts and nutritional implications. We investigated geophagia in the black population of rural Holmes County, Mississippi. Twenty-five sources of geophagical clays were located and most of the sources are associated with rural settlements throughout the county. Clays are taken from subsurface soil horizons, and all but one of the sources come from the upland portion of the county. Geophagia occurs among 57% of women and 16% of children of both sexes, but it is not found among adult males or adolescents. Average daily comsumption of clay is 50 g. Our data indicate geophagia is not correlated with hunger, anemia, or helminthic problems, but it may contribute to the common problem of hypertension. Geophagia has been suggested as one of the factors leading to hyperkalemia, but our data do not support this notion."}
{"id": "PMID:484532", "title": "An evaluation of upper arm measurements used in nutritional assessment.", "content": "Triceps skinfold thickness and upper arm circumference (parameters used in assessing protein-calorie malnutrition) were measured on both arms of 91 adult volunteers who fulfilled criteria for absence of disease and conditions affecting nutritional status. For the total study group and subgroups of men only and women only, no significant differences were noted between right and left arm measurements of triceps skinfold thickness, arm circumference, or arm muscle circumference. Median values for significant right-left arm measurement differences were + 1.7 mm for the triceps skinfold thickness of left-handed subjects (P less than 0.05) and + 0.3 cm for the arm circumference of volunteers regularly engaged in predominately right-armed activities (P less than 0.05). Criteria of weight within 15% of \"ideal\" and nine serum and plasma values within a specified range were fulfilled by 77 subjects, and they comprised the \"healthy\" subgroup. Left arm median values for healthy men and healthy women differed from a currently accepted standard for each of three anthropometric parameters: triceps skinfold thickness (P less than 0.05), arm circumference (P less than 0.05), and arm muscle circumference (P less than 0.01).", "contents": "An evaluation of upper arm measurements used in nutritional assessment. Triceps skinfold thickness and upper arm circumference (parameters used in assessing protein-calorie malnutrition) were measured on both arms of 91 adult volunteers who fulfilled criteria for absence of disease and conditions affecting nutritional status. For the total study group and subgroups of men only and women only, no significant differences were noted between right and left arm measurements of triceps skinfold thickness, arm circumference, or arm muscle circumference. Median values for significant right-left arm measurement differences were + 1.7 mm for the triceps skinfold thickness of left-handed subjects (P less than 0.05) and + 0.3 cm for the arm circumference of volunteers regularly engaged in predominately right-armed activities (P less than 0.05). Criteria of weight within 15% of \"ideal\" and nine serum and plasma values within a specified range were fulfilled by 77 subjects, and they comprised the \"healthy\" subgroup. Left arm median values for healthy men and healthy women differed from a currently accepted standard for each of three anthropometric parameters: triceps skinfold thickness (P less than 0.05), arm circumference (P less than 0.05), and arm muscle circumference (P less than 0.01)."}
{"id": "PMID:484533", "title": "Simultaneous determination of alpha-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography.", "content": "This paper describes a rapid, microprocedure for the simultaneous determination of alpha-tocopherol (vitamin E) and retinol (vitamin A) in plasma, and of alpha-tocopherol alone in red cells since cells do not contain retinol. A total lipid extract from 0.1 ml plasma or 0.125 ml red cells and containing internal standards of alpha-tocopheryl acetate and retinyl acetates is injected onto a high pressure liquid chromatography with a reverse phase column developed with methanol-water. An ultraviolet detector with 280-nm filter is used. The chromatogram is complete in 8 min and the alpha-tocopherol and retinol are quantitated by the peak height ratio method. Comparison of results with both plasma and red cells gave excellent agreement with conventional methods for these vitamins. The procedure should be particularly useful for clinical studies and nutrition surveys.", "contents": "Simultaneous determination of alpha-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. This paper describes a rapid, microprocedure for the simultaneous determination of alpha-tocopherol (vitamin E) and retinol (vitamin A) in plasma, and of alpha-tocopherol alone in red cells since cells do not contain retinol. A total lipid extract from 0.1 ml plasma or 0.125 ml red cells and containing internal standards of alpha-tocopheryl acetate and retinyl acetates is injected onto a high pressure liquid chromatography with a reverse phase column developed with methanol-water. An ultraviolet detector with 280-nm filter is used. The chromatogram is complete in 8 min and the alpha-tocopherol and retinol are quantitated by the peak height ratio method. Comparison of results with both plasma and red cells gave excellent agreement with conventional methods for these vitamins. The procedure should be particularly useful for clinical studies and nutrition surveys."}
{"id": "PMID:484534", "title": "Blood levels of selected nutritional status indicators in young men from a rapidly modernizing society in Abu Dhabi, United Arab Emirates.", "content": "Some blood levels of selected indicators of nutritional status were assessed in young adult males among the rapidly modernizing bedouin society of Abu Dhabi. Blood specimens from 30 indigenous army recruits were tested for the following: packed cell volume, total serum proteins, albumin, globulins, serum transferrin, serum calcium (total and ionized), serum iron, and total folates in serum and in red blood cells. A control group consisted of 10 male expatriate laboratory personnel of similar age. Except for iron and folates, all parameters were within normal range. Although the average serum iron was normal, 13.3% of the army recruits were serum iron deficient. The mean serum folate in both groups fell within the indeterminate range with two deficient recruits. The mean red blood cell folate was in the low normal value in the local people with one-third of them in the borderline range, and two deficiencies with no pathological effects. The control group was well within the acceptable normal range of red blood cell folate and the difference between the means of both samples was significant. Inappropriate amounts of vegetables in the diet and prolonged cooking may be the cause of such folate inadequacy.", "contents": "Blood levels of selected nutritional status indicators in young men from a rapidly modernizing society in Abu Dhabi, United Arab Emirates. Some blood levels of selected indicators of nutritional status were assessed in young adult males among the rapidly modernizing bedouin society of Abu Dhabi. Blood specimens from 30 indigenous army recruits were tested for the following: packed cell volume, total serum proteins, albumin, globulins, serum transferrin, serum calcium (total and ionized), serum iron, and total folates in serum and in red blood cells. A control group consisted of 10 male expatriate laboratory personnel of similar age. Except for iron and folates, all parameters were within normal range. Although the average serum iron was normal, 13.3% of the army recruits were serum iron deficient. The mean serum folate in both groups fell within the indeterminate range with two deficient recruits. The mean red blood cell folate was in the low normal value in the local people with one-third of them in the borderline range, and two deficiencies with no pathological effects. The control group was well within the acceptable normal range of red blood cell folate and the difference between the means of both samples was significant. Inappropriate amounts of vegetables in the diet and prolonged cooking may be the cause of such folate inadequacy."}
{"id": "PMID:484535", "title": "The energy expenditure of Iranian agricultural workers.", "content": "The energy cost of agricultural and standard activities and the daily energy expenditure of male agricultural workers were measured during different seasons in Iranian villages to assess the validity of past and present Food and Agricultural Organization recommended energy allowances for that population. Studies included low income farmers in a village representative of those around the central desert where harvesting takes place under conditions of extreme summer heat. Measurements were also made during the Moslem fasting period when no food may be eaten between dawn and dusk. Energy cost of typical activities was measured by indirect calorimetry using the Max-Planck respirometer and daily energy expenditure was assessed using these figures combined with a diary of activities throughout the 24-hr period. Results of individual activity values are compared with other published figures. Comparison of daily energy expenditure of fasting subjects and nonfasting after Ramazan showed no significant difference. No significant difference was found between values of standardized activities at high summer temperatures and moderate temperatures. Mean values of daily energy expenditure during winter when activity is low are around 2600 kcal/day and for the other seasons of high activity 3400 kcal/day. These figures suggest that past and present Food and Agricultural Organization standards are low for this population.", "contents": "The energy expenditure of Iranian agricultural workers. The energy cost of agricultural and standard activities and the daily energy expenditure of male agricultural workers were measured during different seasons in Iranian villages to assess the validity of past and present Food and Agricultural Organization recommended energy allowances for that population. Studies included low income farmers in a village representative of those around the central desert where harvesting takes place under conditions of extreme summer heat. Measurements were also made during the Moslem fasting period when no food may be eaten between dawn and dusk. Energy cost of typical activities was measured by indirect calorimetry using the Max-Planck respirometer and daily energy expenditure was assessed using these figures combined with a diary of activities throughout the 24-hr period. Results of individual activity values are compared with other published figures. Comparison of daily energy expenditure of fasting subjects and nonfasting after Ramazan showed no significant difference. No significant difference was found between values of standardized activities at high summer temperatures and moderate temperatures. Mean values of daily energy expenditure during winter when activity is low are around 2600 kcal/day and for the other seasons of high activity 3400 kcal/day. These figures suggest that past and present Food and Agricultural Organization standards are low for this population."}
{"id": "PMID:484536", "title": "The chemistry of Chinese drugs.", "content": "Some herbal drugs commonly used in Chinese medicine have been studied chemically in correlation with their biological activities. In many cases it has been recognized that some characteristic chemical constituents could represent the therapeutical actions of the drugs. It is noted that elucidation of their chemical structures in view of the structure-activity relationship provides scientific evidence for the beneficial uses of traditional drugs in health care and, furthermore, promotes the discovering of new therapeutics from them. The principles of Chinese drugs are distributed widely among almost all the chemical groups of natural products, for example, simple phenolics, lignan, flavonoid, quinonoid, lower terpenes, triterpenoid, steroid, true alkaloid, protoalkaloid or amines and simple and polymolecular carbohydrates. In the present article, the chemical principles of some important Chinese drugs currently used are discussed mainly on the basis of our own investigations, and we refer to other works for a general understanding of the present status on the chemical approach to studies of Chinese drugs and medicine.", "contents": "The chemistry of Chinese drugs. Some herbal drugs commonly used in Chinese medicine have been studied chemically in correlation with their biological activities. In many cases it has been recognized that some characteristic chemical constituents could represent the therapeutical actions of the drugs. It is noted that elucidation of their chemical structures in view of the structure-activity relationship provides scientific evidence for the beneficial uses of traditional drugs in health care and, furthermore, promotes the discovering of new therapeutics from them. The principles of Chinese drugs are distributed widely among almost all the chemical groups of natural products, for example, simple phenolics, lignan, flavonoid, quinonoid, lower terpenes, triterpenoid, steroid, true alkaloid, protoalkaloid or amines and simple and polymolecular carbohydrates. In the present article, the chemical principles of some important Chinese drugs currently used are discussed mainly on the basis of our own investigations, and we refer to other works for a general understanding of the present status on the chemical approach to studies of Chinese drugs and medicine."}
{"id": "PMID:484537", "title": "Further study of the neurohumoral factor, endorphin, in the mechanism of acupuncture analgesia.", "content": "Cross circulation was performed between the femoral arteries and veins of paired rabbits that were entirely conscious. Jaw opening reflex by dental pulp stimulation was used as pain index. When acupuncture was applied to the donor rabbit the pain thresholds of both the donor and recipient were elevated from 30 minutes to one hour after acupuncture stimulation and lasted for another 30 minutes after withdrawal of acupuncture. The analgesic effect in both the donor and recipient was abolished by the pretreatment of naloxone. The increase in the pain threshold of the non-acupunctured recipient was due to an opiate-like, humoral substance generated from the acupuncture donor. The possible role of endorphins in the mechanism of acupuncture analgesia is discussed.", "contents": "Further study of the neurohumoral factor, endorphin, in the mechanism of acupuncture analgesia. Cross circulation was performed between the femoral arteries and veins of paired rabbits that were entirely conscious. Jaw opening reflex by dental pulp stimulation was used as pain index. When acupuncture was applied to the donor rabbit the pain thresholds of both the donor and recipient were elevated from 30 minutes to one hour after acupuncture stimulation and lasted for another 30 minutes after withdrawal of acupuncture. The analgesic effect in both the donor and recipient was abolished by the pretreatment of naloxone. The increase in the pain threshold of the non-acupunctured recipient was due to an opiate-like, humoral substance generated from the acupuncture donor. The possible role of endorphins in the mechanism of acupuncture analgesia is discussed."}
{"id": "PMID:484538", "title": "Clinical observations on several meridian loci in chronic hepatitis and liver cirrhosis in comparison with those in female neurovegetative disorder, pregnancy, and the steroid side-effects syndrome.", "content": "Patients with chronic hepatitis showed various grades of the pinch-pressure pain (P-p pain) at the right Ch'\u00fc-Ch'\u00fcan (Li-8) locus. Sometimes the P-p pain also appeared at the left locus (Li-8) as well, but in such cases the tendency is towards a stronger reactivity at the right side than at the left, and the P-p pain does not appear at the left side alone. These findings offer a noticeable contrast to those seen in pregnant women and in steroid-treated patients affected with an obvious side effect where the stronger reactivity is to be seen at the left rather than at the right. The P-p pain grades were inclined to decrease in severe cases, especially in liver cirrhosis. In general, the variability of the P-p pain of grades correlated well with the process of the findings of the liver function test and of the chief subjective complaints. Therefore, the P-p pain test is useful for the diagnosis of chronic hepatitis. Regarding the grade distribution patterns of P-p pain at the Li-8, LI-4, LI-11, and SI-11 loci, patients with chronic hepatitis showed a pattern of higher grades of P-p pain reactivity at the right Li-8 and SI-11 loci, and at the left LI-4 and LI-11 loci, while patients with the so-called female neurovegetative disorder, pregnant women, and patients affected with steroid side-effects showed a perfectly reversed reactivity pattern. These findings can be useful for the differentiation between chronic hepatitis and those groups of diseases showing subjective signs deceptively similar to those of chronic hepatitis and those groups of diseases showing subjective signs deceptively similar to those of chronic hepatitis. The comparison of the skin temperature between the right and the left loci showed lower levels at the right than at the left, and the comparison of the skin pH between the both sides showed a more elevated level at the right than at the left. The mechanisms of these phenomena are discussed and what seems to be the most appropriate explanation at present is offered.", "contents": "Clinical observations on several meridian loci in chronic hepatitis and liver cirrhosis in comparison with those in female neurovegetative disorder, pregnancy, and the steroid side-effects syndrome. Patients with chronic hepatitis showed various grades of the pinch-pressure pain (P-p pain) at the right Ch'\u00fc-Ch'\u00fcan (Li-8) locus. Sometimes the P-p pain also appeared at the left locus (Li-8) as well, but in such cases the tendency is towards a stronger reactivity at the right side than at the left, and the P-p pain does not appear at the left side alone. These findings offer a noticeable contrast to those seen in pregnant women and in steroid-treated patients affected with an obvious side effect where the stronger reactivity is to be seen at the left rather than at the right. The P-p pain grades were inclined to decrease in severe cases, especially in liver cirrhosis. In general, the variability of the P-p pain of grades correlated well with the process of the findings of the liver function test and of the chief subjective complaints. Therefore, the P-p pain test is useful for the diagnosis of chronic hepatitis. Regarding the grade distribution patterns of P-p pain at the Li-8, LI-4, LI-11, and SI-11 loci, patients with chronic hepatitis showed a pattern of higher grades of P-p pain reactivity at the right Li-8 and SI-11 loci, and at the left LI-4 and LI-11 loci, while patients with the so-called female neurovegetative disorder, pregnant women, and patients affected with steroid side-effects showed a perfectly reversed reactivity pattern. These findings can be useful for the differentiation between chronic hepatitis and those groups of diseases showing subjective signs deceptively similar to those of chronic hepatitis and those groups of diseases showing subjective signs deceptively similar to those of chronic hepatitis. The comparison of the skin temperature between the right and the left loci showed lower levels at the right than at the left, and the comparison of the skin pH between the both sides showed a more elevated level at the right than at the left. The mechanisms of these phenomena are discussed and what seems to be the most appropriate explanation at present is offered."}
{"id": "PMID:484539", "title": "The neurophysiological mechanism of acupuncture treatment in psychiatric illness: an autonomic-humoral theory.", "content": "The author traces acupuncture and its development from antiquity to the present time. Based on knowledge and experience so far gathered, an autonomic-humoral theory is proposed to explain the possible mechanism of acupuncture in the treatment of psychiatric conditions. Essentially, the autonomic nervous system and the neurotransmitters are most likely involved. It is hoped that this proposal will stimulate further research in this area.", "contents": "The neurophysiological mechanism of acupuncture treatment in psychiatric illness: an autonomic-humoral theory. The author traces acupuncture and its development from antiquity to the present time. Based on knowledge and experience so far gathered, an autonomic-humoral theory is proposed to explain the possible mechanism of acupuncture in the treatment of psychiatric conditions. Essentially, the autonomic nervous system and the neurotransmitters are most likely involved. It is hoped that this proposal will stimulate further research in this area."}
{"id": "PMID:484540", "title": "Laplace plane analysis of impedance on the H meridian.", "content": "The AC impedance of a length of the H meridian not containing any acupuncture points was studied by means of Laplace plane analysis of the time domain response to an input voltage pulse. The ensuing frequency domain data were compared to the results of an identical analysis for two anatomically similar controls on either side of the meridian. The resistance of the meridian was significantly lower than either control.", "contents": "Laplace plane analysis of impedance on the H meridian. The AC impedance of a length of the H meridian not containing any acupuncture points was studied by means of Laplace plane analysis of the time domain response to an input voltage pulse. The ensuing frequency domain data were compared to the results of an identical analysis for two anatomically similar controls on either side of the meridian. The resistance of the meridian was significantly lower than either control."}
{"id": "PMID:484541", "title": "Reduced ferrochelatase activity in fibroblasts from patients with porphyria variegata.", "content": "Ferrochelatase deficiency has been shown in both porphyria variegata (PV) and erythropoietic protoporphyria (EPP). It has been suggested that in PV there is a decrease in the enzyme, whereas in EPP the enzyme is unstable. In the present study ferrochelatase activity was measured in skin fibroblasts from three patients with PV and three normal subjects. The enzymatic activity in the patients with PV (17.5 +/- 4.5 pmoles heme formed per 10(7) fibroblasts per hour) was 50% of that of the control group (31.0 +/- 3.2 pmoles heme formed per 10(7) fibroblasts per hour). This supports the contention that the enzyme is deficient in PV and that an inactive ferrochelatase is the primary deficiency in this type of porphyria.", "contents": "Reduced ferrochelatase activity in fibroblasts from patients with porphyria variegata. Ferrochelatase deficiency has been shown in both porphyria variegata (PV) and erythropoietic protoporphyria (EPP). It has been suggested that in PV there is a decrease in the enzyme, whereas in EPP the enzyme is unstable. In the present study ferrochelatase activity was measured in skin fibroblasts from three patients with PV and three normal subjects. The enzymatic activity in the patients with PV (17.5 +/- 4.5 pmoles heme formed per 10(7) fibroblasts per hour) was 50% of that of the control group (31.0 +/- 3.2 pmoles heme formed per 10(7) fibroblasts per hour). This supports the contention that the enzyme is deficient in PV and that an inactive ferrochelatase is the primary deficiency in this type of porphyria."}
{"id": "PMID:484543", "title": "Acquired hemoglobin H disease in idiopathic myelofibrosis.", "content": "A 68-year-old male, diagnosed 1 year previously as having myelofibrosis, developed hemolysis, red cell inclusions, and 37% Hb H. The alpha/beta globin synthetic ratio for circulating reticulocytes, determined by 3H-leucine incorporation and globin chain separation by carboxymethylcellulose chromatography in urea, was 0.049. When total RNA was purified from peripheral blood cells and translated in a wheat germ cell-free translation system, the alpha/beta ratio of the translation products was 0.26, indicating mRNA as a major cause of the globin synthetic imbalance. This study demonstrates that myelofibrosis is one setting in which acquired Hb H disease occurs; that the synthetic imbalance may be extreme; and that it can be associated with an imbalance in the activities of specific globin mRNAs.", "contents": "Acquired hemoglobin H disease in idiopathic myelofibrosis. A 68-year-old male, diagnosed 1 year previously as having myelofibrosis, developed hemolysis, red cell inclusions, and 37% Hb H. The alpha/beta globin synthetic ratio for circulating reticulocytes, determined by 3H-leucine incorporation and globin chain separation by carboxymethylcellulose chromatography in urea, was 0.049. When total RNA was purified from peripheral blood cells and translated in a wheat germ cell-free translation system, the alpha/beta ratio of the translation products was 0.26, indicating mRNA as a major cause of the globin synthetic imbalance. This study demonstrates that myelofibrosis is one setting in which acquired Hb H disease occurs; that the synthetic imbalance may be extreme; and that it can be associated with an imbalance in the activities of specific globin mRNAs."}
{"id": "PMID:484547", "title": "A comparison of methods for the rapid quantitation of unsaturated transcobalamin II and the R-binding proteins.", "content": "Methods for the rapid quantitation on unsaturated transcobalamin II (TC II) and R-binders in serum following separation of these binders using a) adsorption by uncoated charcoal, b) adsorption by Quso G32, c) precipitation with ammonium sulphate, and d) acidification of serum, have been compared with the standard gel filtration technique. In serum from healthy subjects and patients with elevated R-binders, the serum acidification and Quso methods yielded results in closest agreement with those obtained by gel filtration. When TC II was markedly elevated, the recommended concentration of Quso failed to remove all TC II from serum. Both the serum acidification and Quso methods are suitable for routine use, providing that with the latter method the concentration of Quso is increased when elevated levels of TC II are anticipated, as, eg, in severe jaundice. All the rapid methods tested yielded clinically significant results in patients with myeloproliferative disorders.", "contents": "A comparison of methods for the rapid quantitation of unsaturated transcobalamin II and the R-binding proteins. Methods for the rapid quantitation on unsaturated transcobalamin II (TC II) and R-binders in serum following separation of these binders using a) adsorption by uncoated charcoal, b) adsorption by Quso G32, c) precipitation with ammonium sulphate, and d) acidification of serum, have been compared with the standard gel filtration technique. In serum from healthy subjects and patients with elevated R-binders, the serum acidification and Quso methods yielded results in closest agreement with those obtained by gel filtration. When TC II was markedly elevated, the recommended concentration of Quso failed to remove all TC II from serum. Both the serum acidification and Quso methods are suitable for routine use, providing that with the latter method the concentration of Quso is increased when elevated levels of TC II are anticipated, as, eg, in severe jaundice. All the rapid methods tested yielded clinically significant results in patients with myeloproliferative disorders."}
{"id": "PMID:484548", "title": "Cold agglutinin autoimmune hemolytic anemia in nonhematologic malignancies.", "content": "Four patients with nonhematologic malignancies had the simultaneous finding of hemolytic anemia due to high-titer cold agglutinins. In each patient, the cold agglutinin had \"anti-I\" specificity and was of the IgM kappa immunoglobulin class. Although patients with hematologic malignancies not uncommonly have cold agglutinins, the association between these antibodies and nonhematologic malignancies is unusual.", "contents": "Cold agglutinin autoimmune hemolytic anemia in nonhematologic malignancies. Four patients with nonhematologic malignancies had the simultaneous finding of hemolytic anemia due to high-titer cold agglutinins. In each patient, the cold agglutinin had \"anti-I\" specificity and was of the IgM kappa immunoglobulin class. Although patients with hematologic malignancies not uncommonly have cold agglutinins, the association between these antibodies and nonhematologic malignancies is unusual."}
{"id": "PMID:484550", "title": "Hereditary mucoepithelial dysplasia: a disease apparently of desmosome and gap junction formation.", "content": "A previously unrecognized autosomal dominant syndrome affecting oral, nasal, vaginal, urethral, anal, bladder, and conjunctival mucosa with cataracts, follicular keratosis, nonscarring alopecia, and terminal lung disease is described in a four-generation kindred of German extraction. Severe photophobia, tearing, and nystagmus in infancy heralds the development of keratitis, corneal vascularization, and lens cataracts. Repeated corneal transplants have failed. Red, periorificial mucosal lesions involving the above structures are noted by 1 year of age and may persist throughout life. Chronic rhinorrhea and repeated upper respiratory infections frequently progress to bilateral pneumonia accompanied by loss of hair, diarrhea, occasional melena, enuresis, pyuria, and hematuria. Spontaneous pneumothorax is frequent, terminating in fibrocystic-type lung disease and cor pulmonale. Women have had repeated abnormal vaginal PAP smears. Histologically the mucosal epithelium shows dyshesion, thinning of the epithelial layer, and dyskeratosis. Mucosal PAP smears show lack of epithelial maturation, cytoplasmic vacuoles and inclusions, and individual cell dyskeratosis. Histochemically there is a lack of cornification and keratinization. Ultrastructural studies show lack of keratohyalin granules, a paucity of desmosomes, intercellular accumulations, cytoplasmic vacuolization, and formation of bands and aggregates of filamentous fibers and structures in the cytoplasm resembling desmosomes and gap junctions. The condition is probably a panepithelial cell defect of desmosomal and gap junction structure most prominently affecting mucosal epithelia associated with an increased susceptibility to a variety of adventitious organisms.", "contents": "Hereditary mucoepithelial dysplasia: a disease apparently of desmosome and gap junction formation. A previously unrecognized autosomal dominant syndrome affecting oral, nasal, vaginal, urethral, anal, bladder, and conjunctival mucosa with cataracts, follicular keratosis, nonscarring alopecia, and terminal lung disease is described in a four-generation kindred of German extraction. Severe photophobia, tearing, and nystagmus in infancy heralds the development of keratitis, corneal vascularization, and lens cataracts. Repeated corneal transplants have failed. Red, periorificial mucosal lesions involving the above structures are noted by 1 year of age and may persist throughout life. Chronic rhinorrhea and repeated upper respiratory infections frequently progress to bilateral pneumonia accompanied by loss of hair, diarrhea, occasional melena, enuresis, pyuria, and hematuria. Spontaneous pneumothorax is frequent, terminating in fibrocystic-type lung disease and cor pulmonale. Women have had repeated abnormal vaginal PAP smears. Histologically the mucosal epithelium shows dyshesion, thinning of the epithelial layer, and dyskeratosis. Mucosal PAP smears show lack of epithelial maturation, cytoplasmic vacuoles and inclusions, and individual cell dyskeratosis. Histochemically there is a lack of cornification and keratinization. Ultrastructural studies show lack of keratohyalin granules, a paucity of desmosomes, intercellular accumulations, cytoplasmic vacuolization, and formation of bands and aggregates of filamentous fibers and structures in the cytoplasm resembling desmosomes and gap junctions. The condition is probably a panepithelial cell defect of desmosomal and gap junction structure most prominently affecting mucosal epithelia associated with an increased susceptibility to a variety of adventitious organisms."}
{"id": "PMID:484551", "title": "Apparent hexosaminidase B deficiency in two healthy members of a pedigree.", "content": "A family is described in which all members have decreased serum and leukocyte hexosaminidase activity. Two individuals, the mother and the younger daughter, have a normal ratio of hexosaminidase B (HEX B) to total hexosaminidase, but their serum enzymes display respectively partial or complete lability to heat. It is proposed that the proband is a double heterozygote for the Sandhoff allele and for an allele producing thermolabile beta subunits.", "contents": "Apparent hexosaminidase B deficiency in two healthy members of a pedigree. A family is described in which all members have decreased serum and leukocyte hexosaminidase activity. Two individuals, the mother and the younger daughter, have a normal ratio of hexosaminidase B (HEX B) to total hexosaminidase, but their serum enzymes display respectively partial or complete lability to heat. It is proposed that the proband is a double heterozygote for the Sandhoff allele and for an allele producing thermolabile beta subunits."}
{"id": "PMID:484552", "title": "Argininosuccinic aciduria: prenatal studies in a family at risk.", "content": "We have monitored two successive pregnancies in a family which we found to be at risk for argininosuccinic aciduria. We measured argininosuccinic acid (ASA) concentrations in amniotic fluid and utilized an indirect assay of ASA lyase activity in cultured amniotic fluid cells. The assay procedure is based on the uptake of 14C from [14C]citrulline and of [3H]leucine into protein. ASA was easily measured in amniotic fluid from the first fetus at risk, whereas none was detectable in control fluids. Amniotic fluid cells cultured from this fetus had only 5.5% of control ASA lyase activity. The pregnancy was terminated, and hepatic ASA lyase activity in the fetus was shown to be about 1.3% of control values. In addition, eight fetal tissues were analyzed for ASA, and all had significant accumulation. ASA was not detected in amniotic fluid from the second fetus at risk, and ASA lyase activity in cultured cells was 80% of control activity. Enzymatic analysis of erythrocyte lysate confirmed the diagnosis of an unaffected child (ASA lyase = 46% of control) and indicated heterozygosity. Thus, we provide further evidence that argininosuccinic aciduria can be diagnosed successfully in utero by indirect assay of ASA lyase activity in cultured amniotic fluid cells. In addition, high amniotic fluid ASA concentrations provide strong adjunctive evidence for such a prenatal determination, and may prove to be sufficient for diagnosis.", "contents": "Argininosuccinic aciduria: prenatal studies in a family at risk. We have monitored two successive pregnancies in a family which we found to be at risk for argininosuccinic aciduria. We measured argininosuccinic acid (ASA) concentrations in amniotic fluid and utilized an indirect assay of ASA lyase activity in cultured amniotic fluid cells. The assay procedure is based on the uptake of 14C from [14C]citrulline and of [3H]leucine into protein. ASA was easily measured in amniotic fluid from the first fetus at risk, whereas none was detectable in control fluids. Amniotic fluid cells cultured from this fetus had only 5.5% of control ASA lyase activity. The pregnancy was terminated, and hepatic ASA lyase activity in the fetus was shown to be about 1.3% of control values. In addition, eight fetal tissues were analyzed for ASA, and all had significant accumulation. ASA was not detected in amniotic fluid from the second fetus at risk, and ASA lyase activity in cultured cells was 80% of control activity. Enzymatic analysis of erythrocyte lysate confirmed the diagnosis of an unaffected child (ASA lyase = 46% of control) and indicated heterozygosity. Thus, we provide further evidence that argininosuccinic aciduria can be diagnosed successfully in utero by indirect assay of ASA lyase activity in cultured amniotic fluid cells. In addition, high amniotic fluid ASA concentrations provide strong adjunctive evidence for such a prenatal determination, and may prove to be sufficient for diagnosis."}
{"id": "PMID:484553", "title": "Late replicating X chromosomes in human triploidy.", "content": "The status of X-chromosome replication was studied in twenty-seven 69,XXY and nine 69,XXX human triploids in which the parental origin of the additional haploid set was known from the study of chromosome heteromorphisms. Among the 69,XXY triploids, fourteen had no late replicating X, two had one late replicating X in all cells examined, and eleven had two populations of cells, one with late replicating X chromosome, and one without any. Among the 69,XXX triploids, four had a single late replicating X, and five had two populations of cells, one with one late replicating X, and one with two late replicating X chromosomes. There was no correlation between the parental origin of the triploidy and the type of X-chromosome inactivation. However the number of late replicating X chromosomes was significantly lower in cultures grown from fetal tissue when compared with those grown from extra-embryonic tissue. In cultures derived from extra-embryonic tissue there was a significant correlation between the gestational age of the sample and the proportion of late replicating X chromosomes. The older the specimen, the greater the number of late replicating X chromosomes.", "contents": "Late replicating X chromosomes in human triploidy. The status of X-chromosome replication was studied in twenty-seven 69,XXY and nine 69,XXX human triploids in which the parental origin of the additional haploid set was known from the study of chromosome heteromorphisms. Among the 69,XXY triploids, fourteen had no late replicating X, two had one late replicating X in all cells examined, and eleven had two populations of cells, one with late replicating X chromosome, and one without any. Among the 69,XXX triploids, four had a single late replicating X, and five had two populations of cells, one with one late replicating X, and one with two late replicating X chromosomes. There was no correlation between the parental origin of the triploidy and the type of X-chromosome inactivation. However the number of late replicating X chromosomes was significantly lower in cultures grown from fetal tissue when compared with those grown from extra-embryonic tissue. In cultures derived from extra-embryonic tissue there was a significant correlation between the gestational age of the sample and the proportion of late replicating X chromosomes. The older the specimen, the greater the number of late replicating X chromosomes."}
{"id": "PMID:484554", "title": "Electrophoresis of glucocerebrosidase from normal and Gaucher disease fibroblasts.", "content": "A polyacrylamide gel electrophoresis system for glucocerebrosidase has been developed. This method was used to characterize the glucocerebrosidase activity of normal and Gaucher disease fibroblasts; the residual glucocerebrosidase activity in adult Gaucher disease fibroblasts co-migrates with the activity from normal fibroblasts.", "contents": "Electrophoresis of glucocerebrosidase from normal and Gaucher disease fibroblasts. A polyacrylamide gel electrophoresis system for glucocerebrosidase has been developed. This method was used to characterize the glucocerebrosidase activity of normal and Gaucher disease fibroblasts; the residual glucocerebrosidase activity in adult Gaucher disease fibroblasts co-migrates with the activity from normal fibroblasts."}
{"id": "PMID:484565", "title": "Effect of an antacid on gastrointestinal absorption of theophylline.", "content": "The effect of a magnesium-aluminum hydroxide antacid (Maalox) on the oral absorption of aminophylline tablets was studied. Twelve healthy adults were administered 200 mg of aminophylline alone or with 30 ml of antacid in a complete crossover study. Blood samples were drawn at 0.33, 0.67, 1, 2, 4, 8, 12, and 24 hours following theophylline (as aminophylline) administration. Theophylline plasma levels were measured by high-performance liquid chromatography. The plasma theophylline concentrations of the group receiving theophylline only were significantly greater than those of the group receiving theophylline plus antacid at the 0.67- and 1-hour sample times only (p less than 0.05). The extent of theophylline absorption and the eliminated rate constant were not significantly affected by the antacid. Antacid significantly decreased theophylline's absorption rate constant (p less than 0.05), indicating a slower absorption of theophylline with antacid. Concurrent administration of the antacid Maalox should not significantly change theophylline's clinical effect.", "contents": "Effect of an antacid on gastrointestinal absorption of theophylline. The effect of a magnesium-aluminum hydroxide antacid (Maalox) on the oral absorption of aminophylline tablets was studied. Twelve healthy adults were administered 200 mg of aminophylline alone or with 30 ml of antacid in a complete crossover study. Blood samples were drawn at 0.33, 0.67, 1, 2, 4, 8, 12, and 24 hours following theophylline (as aminophylline) administration. Theophylline plasma levels were measured by high-performance liquid chromatography. The plasma theophylline concentrations of the group receiving theophylline only were significantly greater than those of the group receiving theophylline plus antacid at the 0.67- and 1-hour sample times only (p less than 0.05). The extent of theophylline absorption and the eliminated rate constant were not significantly affected by the antacid. Antacid significantly decreased theophylline's absorption rate constant (p less than 0.05), indicating a slower absorption of theophylline with antacid. Concurrent administration of the antacid Maalox should not significantly change theophylline's clinical effect."}
{"id": "PMID:484566", "title": "Aminoglycoside serum assays restricted through a pharmacy program.", "content": "A pharmacy program designed to reduce the inappropriate use of aminoglycoside serum assays is described, and the results of an evaluation of the program are reported. If the clinical status of the patient for whom an assay is ordered does not correspond to the indications for the test, a pharmacist calculates predicted aminoglycoside serum levels based on renal function and discourages the physician from having the test performed. If the indications for a test are met, the pharmacist checks for potential interactions. He specifies the exact times serum samples are to be collected, and he prepares a pharmacokinetic report for the physician based on the test results. During the 18-month evaluation, 67 tests were ordered for 53 patients. Three tests were not performed based on pharmacist advice. Five tests could not be evaluated. Of the remaining 59 tests, 56 (95% were used appropriately; 54 of the appropriate decisions made (92%) were a result of the physician following the recommendations of the pharmacist. The program appears to prevent problems that occur when aminoglycoside serum assays are not restricted.", "contents": "Aminoglycoside serum assays restricted through a pharmacy program. A pharmacy program designed to reduce the inappropriate use of aminoglycoside serum assays is described, and the results of an evaluation of the program are reported. If the clinical status of the patient for whom an assay is ordered does not correspond to the indications for the test, a pharmacist calculates predicted aminoglycoside serum levels based on renal function and discourages the physician from having the test performed. If the indications for a test are met, the pharmacist checks for potential interactions. He specifies the exact times serum samples are to be collected, and he prepares a pharmacokinetic report for the physician based on the test results. During the 18-month evaluation, 67 tests were ordered for 53 patients. Three tests were not performed based on pharmacist advice. Five tests could not be evaluated. Of the remaining 59 tests, 56 (95% were used appropriately; 54 of the appropriate decisions made (92%) were a result of the physician following the recommendations of the pharmacist. The program appears to prevent problems that occur when aminoglycoside serum assays are not restricted."}
{"id": "PMID:484567", "title": "Comparative auditory toxicity of aminoglycoside antibiotics in leukopenic patients.", "content": "The incidence of auditory toxicity resulting from therapy with ticarcillin (T) (12 g/sq m/day) in combination with gentamicin (G) (200 mg/sq m/day), amikacin (A) (600 mg/dq m/day), or netilmicin (N) (280 mg/sq m/day) was compared. Before administration of these antibiotic combinations to febrile, granulocytopenic patients, baseline audiograms were determined by a pharmacist using a portable audiometer. The before-therapy audiograms for 32 patients receiving T and G, 29 receiving T and A, and 29 receiving T and N were compared with the audiograms after the completion of therapy. The mean length of therapy was seven days. Two patients (6.2%) receiving T and G, one (3.4%) receiving T and N, and one (3.4%) receiving T and A developed auditory toxicity with bilateral decreases of at least 20 decibels at one or more frequencies. The incidence of auditory toxicity secondary to aminoglycoside exposure was low, and the relative auditory toxicity among the three aminoglycosides appeared to be similar.", "contents": "Comparative auditory toxicity of aminoglycoside antibiotics in leukopenic patients. The incidence of auditory toxicity resulting from therapy with ticarcillin (T) (12 g/sq m/day) in combination with gentamicin (G) (200 mg/sq m/day), amikacin (A) (600 mg/dq m/day), or netilmicin (N) (280 mg/sq m/day) was compared. Before administration of these antibiotic combinations to febrile, granulocytopenic patients, baseline audiograms were determined by a pharmacist using a portable audiometer. The before-therapy audiograms for 32 patients receiving T and G, 29 receiving T and A, and 29 receiving T and N were compared with the audiograms after the completion of therapy. The mean length of therapy was seven days. Two patients (6.2%) receiving T and G, one (3.4%) receiving T and N, and one (3.4%) receiving T and A developed auditory toxicity with bilateral decreases of at least 20 decibels at one or more frequencies. The incidence of auditory toxicity secondary to aminoglycoside exposure was low, and the relative auditory toxicity among the three aminoglycosides appeared to be similar."}
{"id": "PMID:484568", "title": "Clinical toxicology consultation by pharmacists.", "content": "The functions of pharmacists in a clinical toxicology consultation service are described. Pharmacists provided three basic services in conjunction with poisonings treated by the emergency department of a children's hospital: (1) assisted with obtaining the history and assessment of the toxicologic proglems, (2) recommended a plan for rational management, and (3) discussed poison prevention with the parents of the victims. Pharmacists were consulted for 189 poisoning cases over a six-month period; 80% of the cases were attended at the bedside and the remainder were monitored by telephone. Drugs were involved in 58% of the patient exposures. Median time for the pharmacist to reach the emergency room after the patient's arrival was 5 to 10 minutes. Physicians and nurses rated the pharmacists' contributions favorably. These results suggest that pharmacists can play an important role in clinical toxicology.", "contents": "Clinical toxicology consultation by pharmacists. The functions of pharmacists in a clinical toxicology consultation service are described. Pharmacists provided three basic services in conjunction with poisonings treated by the emergency department of a children's hospital: (1) assisted with obtaining the history and assessment of the toxicologic proglems, (2) recommended a plan for rational management, and (3) discussed poison prevention with the parents of the victims. Pharmacists were consulted for 189 poisoning cases over a six-month period; 80% of the cases were attended at the bedside and the remainder were monitored by telephone. Drugs were involved in 58% of the patient exposures. Median time for the pharmacist to reach the emergency room after the patient's arrival was 5 to 10 minutes. Physicians and nurses rated the pharmacists' contributions favorably. These results suggest that pharmacists can play an important role in clinical toxicology."}
{"id": "PMID:484570", "title": "Comparison of kaolin-pectin and activated charcoal for inhibition of aspirin absorption.", "content": "The effects of kaolin-pectin suspension and of activated charcoal on aspirin absorption were compared. Ten fasting volunteers each received on five separate occasions three 325-mg aspirin tablets with: (1) 240 ml of water, (2) 10 g of activated charcoal in a slurry with 240 ml of water, (3) 30 ml of kaolin-pectin suspension with 210 ml of water, (4) 60 ml of kaolin-pectin with 180 ml of water, and (5) 90 ml of kaolin-pectin with 150 ml of water. Aspirin bioavailability was estimated from spectrophotometric assay of total 48-hour urinary salicylate recovery. The mean urine salicylate recovery following administration of activated charcoal (69.5%) was significantly less (p less than 0.01) than that following administration of 30, 60 or 90 ml of kaolin-pectin (90.6, 94.6 and 95.3%, respectively) or of water only (98.6%). The mean percent aspirin recoveries for the 30-ml and 60-ml kaolin-pectin treatments were significantly less than that for water only (p less than 0.05). Neither activated charcoal nor kaolin-pectin delayed the rate of aspirin absorption. Although kaolin-pectin reduces the absorption of aspirin, the effect would be of marginal clinical importance. Kaolin-pectin suspension is not recommended as a treatment for aspirin poisoning.", "contents": "Comparison of kaolin-pectin and activated charcoal for inhibition of aspirin absorption. The effects of kaolin-pectin suspension and of activated charcoal on aspirin absorption were compared. Ten fasting volunteers each received on five separate occasions three 325-mg aspirin tablets with: (1) 240 ml of water, (2) 10 g of activated charcoal in a slurry with 240 ml of water, (3) 30 ml of kaolin-pectin suspension with 210 ml of water, (4) 60 ml of kaolin-pectin with 180 ml of water, and (5) 90 ml of kaolin-pectin with 150 ml of water. Aspirin bioavailability was estimated from spectrophotometric assay of total 48-hour urinary salicylate recovery. The mean urine salicylate recovery following administration of activated charcoal (69.5%) was significantly less (p less than 0.01) than that following administration of 30, 60 or 90 ml of kaolin-pectin (90.6, 94.6 and 95.3%, respectively) or of water only (98.6%). The mean percent aspirin recoveries for the 30-ml and 60-ml kaolin-pectin treatments were significantly less than that for water only (p less than 0.05). Neither activated charcoal nor kaolin-pectin delayed the rate of aspirin absorption. Although kaolin-pectin reduces the absorption of aspirin, the effect would be of marginal clinical importance. Kaolin-pectin suspension is not recommended as a treatment for aspirin poisoning."}
{"id": "PMID:484571", "title": "Relative drug safety and efficacy: any help for the practitioner?", "content": "Approaches are discussed for assuring that the most useful entity is prescribed when a drug is the treatment of choice. Several attributes of pharmaceutical preparations that are likely to influence health status are mentioned. Three models for increasing drug-of-choice prescribing are discussed: the regulatory model, the formulary model, and the drug use review model. Four options regarding formulary preparation are presented: the federal government, a consortium of professional groups, regional expert committees, or local groups. Research is needed to determine which methods hold promise for increasing the frequency of rational prescribing.", "contents": "Relative drug safety and efficacy: any help for the practitioner? Approaches are discussed for assuring that the most useful entity is prescribed when a drug is the treatment of choice. Several attributes of pharmaceutical preparations that are likely to influence health status are mentioned. Three models for increasing drug-of-choice prescribing are discussed: the regulatory model, the formulary model, and the drug use review model. Four options regarding formulary preparation are presented: the federal government, a consortium of professional groups, regional expert committees, or local groups. Research is needed to determine which methods hold promise for increasing the frequency of rational prescribing."}
{"id": "PMID:484579", "title": "A systematic procedure for selection of automated instruments in the clinical laboratory.", "content": "A five-step systematic procedure for comparison and selection of automated instruments for the clinical laboratory is presented. Thirteen criteria commonly used for selection of instruments in the clinical laboratory are discussed, and from these an opinion survey form is developed for use in decision making. A procedure for using this opinion survey form, tabulating the survey data, and making the final recommendation is also discussed.", "contents": "A systematic procedure for selection of automated instruments in the clinical laboratory. A five-step systematic procedure for comparison and selection of automated instruments for the clinical laboratory is presented. Thirteen criteria commonly used for selection of instruments in the clinical laboratory are discussed, and from these an opinion survey form is developed for use in decision making. A procedure for using this opinion survey form, tabulating the survey data, and making the final recommendation is also discussed."}
{"id": "PMID:484586", "title": "The effect of hematocrit on visual platelet estimation.", "content": "Estimation of the number of platelets on Wright's-stained blood smear and from phase platelet counts on more than 1700 patients were analyzed to determine if there were statistical significance in various ranges of hematocrit values. F-test analysis showed no significant difference among these variations in hematocrit determinations.", "contents": "The effect of hematocrit on visual platelet estimation. Estimation of the number of platelets on Wright's-stained blood smear and from phase platelet counts on more than 1700 patients were analyzed to determine if there were statistical significance in various ranges of hematocrit values. F-test analysis showed no significant difference among these variations in hematocrit determinations."}
{"id": "PMID:484587", "title": "Safety procedures in clinical laboratories.", "content": "Five types of hazards encountered in the clinical laboratory and a list of safety procedures designed to avoid or minimize them are identified. The safety procedures are written in a precise and easy-to-follow manner so that they can be adopted and used in any clinical laboratory. Medical technology educators are encouraged to teach safety procedures and enforce them in the student laboratory.", "contents": "Safety procedures in clinical laboratories. Five types of hazards encountered in the clinical laboratory and a list of safety procedures designed to avoid or minimize them are identified. The safety procedures are written in a precise and easy-to-follow manner so that they can be adopted and used in any clinical laboratory. Medical technology educators are encouraged to teach safety procedures and enforce them in the student laboratory."}
{"id": "PMID:484594", "title": "The association of Waardenburg syndrome and Hirschsprung megacolon.", "content": "We describe four patients with Waardenburg syndrome and Hirschsprung aganglionic megacolon. In view of pathophysiologic relationships and animal studies, we conclude that the association of these two uncommon disorders is pathophysiologically significant.", "contents": "The association of Waardenburg syndrome and Hirschsprung megacolon. We describe four patients with Waardenburg syndrome and Hirschsprung aganglionic megacolon. In view of pathophysiologic relationships and animal studies, we conclude that the association of these two uncommon disorders is pathophysiologically significant."}
{"id": "PMID:484590", "title": "Comparison of noninvasive with hemodynamic data in patients with pulmonary hypertension due to chronic obstructive pulmonary disease.", "content": "Twenty-one patients with chronic obstructive pulmonary disease were studied with right heart catheterization. The mean pulmonary artery pressure (PAP) was compared with several noninvasive tests. The pulmonary lobar diameter/thoracic ratio correlated with the PAP, r = 0.677, P less than 0.01. The oxygen saturation and pH were used to derive a calculated value for PAP. The calculated PAP correlated with PAP values measured below 40 mm Hg, r = 0.787, P less than 0.01 but correlated very poorly with PAP values measured above 40 mm Hg. The electrocardiogram revealed at least one criterion for right ventricular abnormality in all 18 patients with pulmonary hypertension, but was too nonspecific to correlate with PAP. The hemoglobin concentration did not correlate with PAP. There was such wide variation between the measured PAP and the various noninvasive tests that these noninvasive tests could not be used to accurately predict the PAP in an individual patient.", "contents": "Comparison of noninvasive with hemodynamic data in patients with pulmonary hypertension due to chronic obstructive pulmonary disease. Twenty-one patients with chronic obstructive pulmonary disease were studied with right heart catheterization. The mean pulmonary artery pressure (PAP) was compared with several noninvasive tests. The pulmonary lobar diameter/thoracic ratio correlated with the PAP, r = 0.677, P less than 0.01. The oxygen saturation and pH were used to derive a calculated value for PAP. The calculated PAP correlated with PAP values measured below 40 mm Hg, r = 0.787, P less than 0.01 but correlated very poorly with PAP values measured above 40 mm Hg. The electrocardiogram revealed at least one criterion for right ventricular abnormality in all 18 patients with pulmonary hypertension, but was too nonspecific to correlate with PAP. The hemoglobin concentration did not correlate with PAP. There was such wide variation between the measured PAP and the various noninvasive tests that these noninvasive tests could not be used to accurately predict the PAP in an individual patient."}
{"id": "PMID:484596", "title": "The Bowen-Conradi syndrome -- a highly lethal autosomal recessive syndrome of microcephaly, micrognathia, low birth weight, and joint deformities.", "content": "This paper describes six Hutterite children from five families who appear to have been affected by the same syndrome that was described in two brothers by Bowen and Conradi [1]. Our additional cases confirm that the major features of the syndrome include porportionate intrauterine growth retardation, microcephaly, micrognathia, a prominent nose, rocker-bottom feet, joint limitation, and failure to thrive, with death within the first year of life. Bowen-Conradi syndrome is an autosomal recessive trait and pedigree records show that all six families now known are related to each other through two couples born in the late 1700s but that there are additional earlier possible sources of the responsible gene. The differential diagnosis of this syndrome is discussed.", "contents": "The Bowen-Conradi syndrome -- a highly lethal autosomal recessive syndrome of microcephaly, micrognathia, low birth weight, and joint deformities. This paper describes six Hutterite children from five families who appear to have been affected by the same syndrome that was described in two brothers by Bowen and Conradi [1]. Our additional cases confirm that the major features of the syndrome include porportionate intrauterine growth retardation, microcephaly, micrognathia, a prominent nose, rocker-bottom feet, joint limitation, and failure to thrive, with death within the first year of life. Bowen-Conradi syndrome is an autosomal recessive trait and pedigree records show that all six families now known are related to each other through two couples born in the late 1700s but that there are additional earlier possible sources of the responsible gene. The differential diagnosis of this syndrome is discussed."}
{"id": "PMID:484591", "title": "Serum immunoglobulins in chronic lymphocytic leukemia.", "content": "Serum immunoglobulin levels were periodically determined in 70 CLL patients and the changes were correlated with several clinical and laboratory parameters. It was found that the IgG and IgA levels decreased significantly as the disease progressed. A low IgG concentration was found at the time of diagnosis in 18.7% and after six years in about 50% of the patients. The IgM concentration, although initially low, increased during the follow-up in 43% of the patients and in four of them a monoclonal fraction appeared in the serum. The changes in the immunoglobulins did not correlate with age, sex or initial leukocyte count. Stage O patients as well as untreated patients also had a decrease in their immunoglobulin levels but advanced disease stage and especially continuous chemotherapy seemed to augment the drop in the immunoglobulin levels. Neither the initial immunoglobulin levels nor the subsequent changes, absolute or relative, had a significant prognostic value.", "contents": "Serum immunoglobulins in chronic lymphocytic leukemia. Serum immunoglobulin levels were periodically determined in 70 CLL patients and the changes were correlated with several clinical and laboratory parameters. It was found that the IgG and IgA levels decreased significantly as the disease progressed. A low IgG concentration was found at the time of diagnosis in 18.7% and after six years in about 50% of the patients. The IgM concentration, although initially low, increased during the follow-up in 43% of the patients and in four of them a monoclonal fraction appeared in the serum. The changes in the immunoglobulins did not correlate with age, sex or initial leukocyte count. Stage O patients as well as untreated patients also had a decrease in their immunoglobulin levels but advanced disease stage and especially continuous chemotherapy seemed to augment the drop in the immunoglobulin levels. Neither the initial immunoglobulin levels nor the subsequent changes, absolute or relative, had a significant prognostic value."}
{"id": "PMID:484598", "title": "Sporadic case of apparent aprosencephaly.", "content": "We report a sporadic case of apparent aprosencephaly, ie, apparent absence of forebrain with the facial anomalies of the (alobar) holoprosencephaly field complex. The infant lived-14 months, manifested temperature variations between 34.5 and 41.7 degrees C, and suffered at least one episode of severe hyponatremia suggesting hypoaldosteronism of brain or adrenal origin. Eye changes were suggestive of Rieger mesodermal dysgenesis.", "contents": "Sporadic case of apparent aprosencephaly. We report a sporadic case of apparent aprosencephaly, ie, apparent absence of forebrain with the facial anomalies of the (alobar) holoprosencephaly field complex. The infant lived-14 months, manifested temperature variations between 34.5 and 41.7 degrees C, and suffered at least one episode of severe hyponatremia suggesting hypoaldosteronism of brain or adrenal origin. Eye changes were suggestive of Rieger mesodermal dysgenesis."}
{"id": "PMID:484599", "title": "Are retarded children more distractible? Observational analysis of retarded and nonretarded children's classroom behavior.", "content": "Retarded children are frequently characterized as more distractible and/or more outerdirected than are nonretarded children. In this study behavior thought to reflect these processes was described and the relationship of this kind of behavior to task demands was examined. Retarded and nonretarded children were observed while they worked on academic classroom tasks; retarded children were also observed while they worked on nonacademic tasks. Results indicated that retarded children were less attentive than were nonretarded children during academic periods: They spent less time on task, more time out of their seats, and more time \"looking busy\" but not working. Little support was found for the notion that retarded children are more outerdirected than are nonretarded children. Retarded children responded differently in nonacademic and academic situations, suggesting that behavior reflecting attention is task-related. Results were discussed in terms of previous conceptualizations of retarded persons' attention processes. Implications for future research and for educational practice were also discussed.", "contents": "Are retarded children more distractible? Observational analysis of retarded and nonretarded children's classroom behavior. Retarded children are frequently characterized as more distractible and/or more outerdirected than are nonretarded children. In this study behavior thought to reflect these processes was described and the relationship of this kind of behavior to task demands was examined. Retarded and nonretarded children were observed while they worked on academic classroom tasks; retarded children were also observed while they worked on nonacademic tasks. Results indicated that retarded children were less attentive than were nonretarded children during academic periods: They spent less time on task, more time out of their seats, and more time \"looking busy\" but not working. Little support was found for the notion that retarded children are more outerdirected than are nonretarded children. Retarded children responded differently in nonacademic and academic situations, suggesting that behavior reflecting attention is task-related. Results were discussed in terms of previous conceptualizations of retarded persons' attention processes. Implications for future research and for educational practice were also discussed."}
{"id": "PMID:484600", "title": "What retarded adults believe about sex.", "content": "Information was presented from results of administration of the Socio-Knowledge and Attitudes Test to retarded persons aged 18 to 41 (equal sex distribution), most of whom were moderately or severely retarded. One-half of the sample were residents in a state institution, and the other half lived in their own or in group homes in an urban community. Little relationship was found between subjects' ages and response scores, but, on certain subtests, there were significant relationships between sex-knowledge score and subject's IQ, adaptive behavior level, sex, and/or place of residence.", "contents": "What retarded adults believe about sex. Information was presented from results of administration of the Socio-Knowledge and Attitudes Test to retarded persons aged 18 to 41 (equal sex distribution), most of whom were moderately or severely retarded. One-half of the sample were residents in a state institution, and the other half lived in their own or in group homes in an urban community. Little relationship was found between subjects' ages and response scores, but, on certain subtests, there were significant relationships between sex-knowledge score and subject's IQ, adaptive behavior level, sex, and/or place of residence."}
{"id": "PMID:484602", "title": "Effects of learning to ski on the self-concept of mentally retarded children.", "content": "Changes in the self-concept of TMR children as a function of experience in a physical activity program were evaluated. The self-concept of a group of students was measured before and after participation in a 5-week ski program. A control group received similar pre- and postmeasures of self-concept but did not participate in the ski program. Significant changes in self-concept occurred among students in the experimental but not the control group. Furthermore, the magnitude of success in learning to ski was shown to be positively and significantly correlated with magnitude of change in self-concept.", "contents": "Effects of learning to ski on the self-concept of mentally retarded children. Changes in the self-concept of TMR children as a function of experience in a physical activity program were evaluated. The self-concept of a group of students was measured before and after participation in a 5-week ski program. A control group received similar pre- and postmeasures of self-concept but did not participate in the ski program. Significant changes in self-concept occurred among students in the experimental but not the control group. Furthermore, the magnitude of success in learning to ski was shown to be positively and significantly correlated with magnitude of change in self-concept."}
{"id": "PMID:484603", "title": "Cross-organizational job satisfactions of technician-level staff members.", "content": "Job satisfactions of technicians were assessed in three medium to large public residential facilities using the Job Descriptive Index. Technicians in a fourth, much smaller facility provided separate reliability and validity data on the Job Descriptive Index. In general, the job satisfactions of technicians as measured by this scale were lower than those of workers in industrial settings, particularly with respect to pay. Correlational analyses revealed no significant relationship between client characteristics and satisfaction scores. Staffing patterns did, however, affect technician satisfactions in several areas.", "contents": "Cross-organizational job satisfactions of technician-level staff members. Job satisfactions of technicians were assessed in three medium to large public residential facilities using the Job Descriptive Index. Technicians in a fourth, much smaller facility provided separate reliability and validity data on the Job Descriptive Index. In general, the job satisfactions of technicians as measured by this scale were lower than those of workers in industrial settings, particularly with respect to pay. Correlational analyses revealed no significant relationship between client characteristics and satisfaction scores. Staffing patterns did, however, affect technician satisfactions in several areas."}
{"id": "PMID:484592", "title": "Age and sex dependency of the Achilles tendon reflex.", "content": "Achilles tendon reflex time was measured in normal subjects (1,837 females and 7,937 males). Values of half relaxation time showed a clear dependency on both age and sex. The reflex becomes slower with advancing age and was consistently slower in females. The results redefine the normal range of the Achilles tendon reflex. Regression lines of relaxation time on age were fitted for each sex separately. Lines representing linear regression, log-linear regression or quadratic regression were inferior to those described by linear splines. The gradual increase of Achilles tendon relaxation time with age was not due to unrecognized hypothyroidism or peripheral neuropathy. It is suggested that one cause of the progressive increment is diminution of sympathetic tone with age, and changes in the muscles themselves may also contribute.", "contents": "Age and sex dependency of the Achilles tendon reflex. Achilles tendon reflex time was measured in normal subjects (1,837 females and 7,937 males). Values of half relaxation time showed a clear dependency on both age and sex. The reflex becomes slower with advancing age and was consistently slower in females. The results redefine the normal range of the Achilles tendon reflex. Regression lines of relaxation time on age were fitted for each sex separately. Lines representing linear regression, log-linear regression or quadratic regression were inferior to those described by linear splines. The gradual increase of Achilles tendon relaxation time with age was not due to unrecognized hypothyroidism or peripheral neuropathy. It is suggested that one cause of the progressive increment is diminution of sympathetic tone with age, and changes in the muscles themselves may also contribute."}
{"id": "PMID:484604", "title": "Technician losses in public residential facilities.", "content": "A cross-organizational longitudinal survey was undertaken of state-operated residential facilities to gauge the extent to which technician-level work forces are affected by turnover and absenteeism. Twelve facilities participated in this study for a period of 9 months. Monthly turnover rates ranged from .6 to 4.9 percent, with a average of 2.4 percent. Turnover rates appeared to be influenced by the size, location, and staffing patterns of the individual institution. With respect to absenteeism, the average \"lost time rate\" was 6.7 percent. Various standards suggest that absenteeism exceeding 4.5 percent disrupts recommended staffing ratios. One may conclude that employee withdrawal, whether in the form of avoidable turnover or unscheduled absenteeism, represents a serious managerial problem for many institutions.", "contents": "Technician losses in public residential facilities. A cross-organizational longitudinal survey was undertaken of state-operated residential facilities to gauge the extent to which technician-level work forces are affected by turnover and absenteeism. Twelve facilities participated in this study for a period of 9 months. Monthly turnover rates ranged from .6 to 4.9 percent, with a average of 2.4 percent. Turnover rates appeared to be influenced by the size, location, and staffing patterns of the individual institution. With respect to absenteeism, the average \"lost time rate\" was 6.7 percent. Various standards suggest that absenteeism exceeding 4.5 percent disrupts recommended staffing ratios. One may conclude that employee withdrawal, whether in the form of avoidable turnover or unscheduled absenteeism, represents a serious managerial problem for many institutions."}
{"id": "PMID:484605", "title": "Iconic-memory processing of unfamiliar stimuli by retarded and nonretarded individuals.", "content": "The iconic-memory processing of unfamiliar stimuli was undertaken employing a visually cued partial-report procedure and a visual masking procedure. Subjects viewed stimulus arrays consisting of six Chinese characters arranged in a circular pattern for 100 msec. At variable stimulus-onset asynchronies, a teardrop indicator or an annulus was presented for 100 msec. Immediately upon cue offset, the subject was required to recognize the cued stimulus from a card containing a single character. Retarded subjects' performance was comparable to that of MA- and CA-matched subjects. We suggested that earlier reported iconic-memory differences between retarded and nonretarded individuals may be attributable to processes other than iconic memory.", "contents": "Iconic-memory processing of unfamiliar stimuli by retarded and nonretarded individuals. The iconic-memory processing of unfamiliar stimuli was undertaken employing a visually cued partial-report procedure and a visual masking procedure. Subjects viewed stimulus arrays consisting of six Chinese characters arranged in a circular pattern for 100 msec. At variable stimulus-onset asynchronies, a teardrop indicator or an annulus was presented for 100 msec. Immediately upon cue offset, the subject was required to recognize the cued stimulus from a card containing a single character. Retarded subjects' performance was comparable to that of MA- and CA-matched subjects. We suggested that earlier reported iconic-memory differences between retarded and nonretarded individuals may be attributable to processes other than iconic memory."}
{"id": "PMID:484606", "title": "Lexical usage of retarded children: an examination of informativeness.", "content": "Retarded children's lexical usage was examined as a function of informativeness. The children were most likely to both label and imitate when the referent objects were informative in the presentation sequence. The frequency of labeling and imitation was particularly high when the informative element was an object exemplar that had not been seen before. The results were discussed in terms of the presuppositional abilities seemingly demonstrated by the children and the implications these abilities have for intervention programs.", "contents": "Lexical usage of retarded children: an examination of informativeness. Retarded children's lexical usage was examined as a function of informativeness. The children were most likely to both label and imitate when the referent objects were informative in the presentation sequence. The frequency of labeling and imitation was particularly high when the informative element was an object exemplar that had not been seen before. The results were discussed in terms of the presuppositional abilities seemingly demonstrated by the children and the implications these abilities have for intervention programs."}
{"id": "PMID:484607", "title": "Speed and accuracy in the choice reaction time of mildly retarded adults.", "content": "Reaction times (RTs) to inside stimuli in an 8-choice task decreased for some retarded subjects when the response ensemble was concealed. Corresponding increases in errors suggested that these subjects made separate visual checks of the response keys in order to respond accurately. Other retarded subjects maintained accurate performance by taking longer to respond. An examination of error data from a number of RT experiments indicated that retarded subjects made more errors than did nonretarded subjects when stimuli and responses were less descriminable. A comparison of RTs for correct and incorrect responses reflected intermittent rather than general tendencies to sacrifice accuracy for speed, a result consistent with the possibility of occasional attentional lapses.", "contents": "Speed and accuracy in the choice reaction time of mildly retarded adults. Reaction times (RTs) to inside stimuli in an 8-choice task decreased for some retarded subjects when the response ensemble was concealed. Corresponding increases in errors suggested that these subjects made separate visual checks of the response keys in order to respond accurately. Other retarded subjects maintained accurate performance by taking longer to respond. An examination of error data from a number of RT experiments indicated that retarded subjects made more errors than did nonretarded subjects when stimuli and responses were less descriminable. A comparison of RTs for correct and incorrect responses reflected intermittent rather than general tendencies to sacrifice accuracy for speed, a result consistent with the possibility of occasional attentional lapses."}
{"id": "PMID:484608", "title": "Prevalence of speech and hearing impairment noninstitutionalized mentally retarded adults.", "content": "The prevalence of speech and hearing impairment was examinated in a sample of 518 mentally retarded persons currently residing in community residential facilities. The prevalence of speech impairment was found to be approximately 51 percent, while hearing impairment was found in about 15 percent of the sample. Prevalence figures were further delineated by level of mental retardation. Speech impairment showed a moderate correlation with measures of intelligence and personal competence. The results of this study support the need of this population for speech and hearing services.", "contents": "Prevalence of speech and hearing impairment noninstitutionalized mentally retarded adults. The prevalence of speech and hearing impairment was examinated in a sample of 518 mentally retarded persons currently residing in community residential facilities. The prevalence of speech impairment was found to be approximately 51 percent, while hearing impairment was found in about 15 percent of the sample. Prevalence figures were further delineated by level of mental retardation. Speech impairment showed a moderate correlation with measures of intelligence and personal competence. The results of this study support the need of this population for speech and hearing services."}
{"id": "PMID:484609", "title": "Extended recency effect extended: blocking, presentation mode, and retention interval.", "content": "The effect of blocking of stimulus items on the free recall of EMR adolescents was examined. In Experiment 1 a multitrial free-recall list of 15 pictures was presented either simultaneously in groups of 3, or sequentially, one at a time. Consistent ordering was used in both conditions, so that on each trial, each item in each set of 3 pictures was presented contiguously with the other 2 items from that set. In addition, recall came immediately or after a filled or unfilled delay of 24.5 seconds. Results showed that simultaneous presentation led to higher recall, subjective organization, and clustering than did sequential presentation, but analysis of serial-position curves showed a much reduced extended recency effect in comparison with previous studies. Experiment 2 was designed to determine whether the cause of the reduced extended recency was the use of pictures rather than words as stimuli. Stimuli were presented either as pictures, as pictures with auditory labels, or as words with auditory labels, with both simultaneous and consistent ordering for all conditions. Results indicated a strong extended recency effect for all groups, eliminating presentation mode as a causal factor in the data of Experiment 1. We concluded that blocking leads to increased organization and recall over a variety of presentation modes, rates, and block sizes.", "contents": "Extended recency effect extended: blocking, presentation mode, and retention interval. The effect of blocking of stimulus items on the free recall of EMR adolescents was examined. In Experiment 1 a multitrial free-recall list of 15 pictures was presented either simultaneously in groups of 3, or sequentially, one at a time. Consistent ordering was used in both conditions, so that on each trial, each item in each set of 3 pictures was presented contiguously with the other 2 items from that set. In addition, recall came immediately or after a filled or unfilled delay of 24.5 seconds. Results showed that simultaneous presentation led to higher recall, subjective organization, and clustering than did sequential presentation, but analysis of serial-position curves showed a much reduced extended recency effect in comparison with previous studies. Experiment 2 was designed to determine whether the cause of the reduced extended recency was the use of pictures rather than words as stimuli. Stimuli were presented either as pictures, as pictures with auditory labels, or as words with auditory labels, with both simultaneous and consistent ordering for all conditions. Results indicated a strong extended recency effect for all groups, eliminating presentation mode as a causal factor in the data of Experiment 1. We concluded that blocking leads to increased organization and recall over a variety of presentation modes, rates, and block sizes."}
{"id": "PMID:484610", "title": "Use of teacher prompts to increase social behavior: generalization effects with severely and profoundly retarded adolescents.", "content": "In a single-subject design with replication across subjects, teacher prompts were used in an attempt to increase the rate of social behavior of three severely and profoundly retarded adolescents who were legally blind. Training took place in the classroom on successive school days with the teacher prompting each subject to engage in positive social interaction with each peer. Observations for generalization effects immediately followed each training session. The remaining class members were brought into the room, and social interactions were observed in a free-play setting while the teacher was absent. During both phases, observers recorded the behavior of the three subjects for 5 minutes and recorded all units of social exchange that each subject initiated or responded to. Increased rates of social behavior were obtained for all three subjects during both training and generalization. Major factors that contributed to the generalization effects were discussed.", "contents": "Use of teacher prompts to increase social behavior: generalization effects with severely and profoundly retarded adolescents. In a single-subject design with replication across subjects, teacher prompts were used in an attempt to increase the rate of social behavior of three severely and profoundly retarded adolescents who were legally blind. Training took place in the classroom on successive school days with the teacher prompting each subject to engage in positive social interaction with each peer. Observations for generalization effects immediately followed each training session. The remaining class members were brought into the room, and social interactions were observed in a free-play setting while the teacher was absent. During both phases, observers recorded the behavior of the three subjects for 5 minutes and recorded all units of social exchange that each subject initiated or responded to. Increased rates of social behavior were obtained for all three subjects during both training and generalization. Major factors that contributed to the generalization effects were discussed."}
{"id": "PMID:484611", "title": "Reducing inappropriate verbalizations of a retarded adult.", "content": "Social feedback was investigated as a treatment strategy for reducing inappropriate verbalizations by a retarded adult in a vocational-training program. Treatment procedures were introduced as a combination of three components: praise for not verbalizing inappropriately, reprimands, and instruction. Additional investigations were made into procedures for withdrawing treatment, while maintaining the corrected verbal behavior. Results of the study indicated that the social-feedback procedures were effective in reducing inappropriate verbalizations. Results of withdrawing all social-feedback components at once suggested that the treatment gains would not be maintained; however, fading social-feedback components sequentially was effective in maintaining low levels of inappropriate verbalizations. Further research into multiple-component training packages for changing and maintaining verbal behavior may provide a means for training more skills leading to job success for mentally retarded adults.", "contents": "Reducing inappropriate verbalizations of a retarded adult. Social feedback was investigated as a treatment strategy for reducing inappropriate verbalizations by a retarded adult in a vocational-training program. Treatment procedures were introduced as a combination of three components: praise for not verbalizing inappropriately, reprimands, and instruction. Additional investigations were made into procedures for withdrawing treatment, while maintaining the corrected verbal behavior. Results of the study indicated that the social-feedback procedures were effective in reducing inappropriate verbalizations. Results of withdrawing all social-feedback components at once suggested that the treatment gains would not be maintained; however, fading social-feedback components sequentially was effective in maintaining low levels of inappropriate verbalizations. Further research into multiple-component training packages for changing and maintaining verbal behavior may provide a means for training more skills leading to job success for mentally retarded adults."}
{"id": "PMID:484613", "title": "Free recall of nonretarded and EMR children: associative and categorical bases of clustering.", "content": "A 12-item stimulus list composed of three conceptual categories, each with two low- and two high-associated word pairs, was presented for free recall. Twenty EMR preadolscents, 20 EMR adolescents, and 20 nonretarded fourth-grade children showed associative clustering in accordance with preexperimental interitem associative strength. The nonretarded children showed significantly greater recall and categorical clustering than did the EMR groups. No groups benefited from instructions to cluster. Nonretarded children do not appear to need outside help to accomplish clustering of categorizable material, whereas retarded persons seem to require stronger interventions to induce organization in recall.", "contents": "Free recall of nonretarded and EMR children: associative and categorical bases of clustering. A 12-item stimulus list composed of three conceptual categories, each with two low- and two high-associated word pairs, was presented for free recall. Twenty EMR preadolscents, 20 EMR adolescents, and 20 nonretarded fourth-grade children showed associative clustering in accordance with preexperimental interitem associative strength. The nonretarded children showed significantly greater recall and categorical clustering than did the EMR groups. No groups benefited from instructions to cluster. Nonretarded children do not appear to need outside help to accomplish clustering of categorizable material, whereas retarded persons seem to require stronger interventions to induce organization in recall."}
{"id": "PMID:484615", "title": "The role of a trial of labor with a positive contraction stress test.", "content": "During a 28 month period 812 patients underwent antepartum FHR testing. Twenty-eight patients had a positive CST. There were two antepartum fetal deaths and 11 patients had a cesarean section without a trial of labor. Fifteen patients had a trial of closely monitored labor (continuous FHR and fetal scalp blood sampling when indicated) and 11 of these (73%) were delivered vaginally. The CST records were examined for: per cent late deceleration, baseline FHR, presence of FHR accelerations, duration of the latency period (time from onset of contraction to onset of deceleration), and amplitude of deceleration. The absence of accelerations (nonreactive CST) and a latency period of less than 45 seconds both predicted persistent late deceleration during labor or fetal death in utero but statistical significance was found only for the latter parameter.", "contents": "The role of a trial of labor with a positive contraction stress test. During a 28 month period 812 patients underwent antepartum FHR testing. Twenty-eight patients had a positive CST. There were two antepartum fetal deaths and 11 patients had a cesarean section without a trial of labor. Fifteen patients had a trial of closely monitored labor (continuous FHR and fetal scalp blood sampling when indicated) and 11 of these (73%) were delivered vaginally. The CST records were examined for: per cent late deceleration, baseline FHR, presence of FHR accelerations, duration of the latency period (time from onset of contraction to onset of deceleration), and amplitude of deceleration. The absence of accelerations (nonreactive CST) and a latency period of less than 45 seconds both predicted persistent late deceleration during labor or fetal death in utero but statistical significance was found only for the latter parameter."}
{"id": "PMID:484616", "title": "Pregnancy in the underweight woman. Course, outcome, and growth patterns of the infant.", "content": "The obstetric performance and pregnancy outcome of 354 underweight patients were compared with matched control subjects of normal weight. The growth patterns of their infants were also compared. The underweight women had significantly higher rates of cardiac/respiratory problems, anemia, PROM, and endometritis but were less prone to develop pre-eclampsia. Prematurity and low Apgar scores were significantly more frequent in the infants of underweight women. There was no difference in the frequency of IUGR and in perinatal mortality rates. The mean birth weight of the infants of underweight women was 231 grams less than that of infants of control subjects. Underweight women, particularly if they were anemic, had a higher incidence of low-birth-weight infants despite adequate weight gain. AGA infants of underweight women were more likely to be below the twenty-fifth percentile for weight correlated with length by 12 months of age.", "contents": "Pregnancy in the underweight woman. Course, outcome, and growth patterns of the infant. The obstetric performance and pregnancy outcome of 354 underweight patients were compared with matched control subjects of normal weight. The growth patterns of their infants were also compared. The underweight women had significantly higher rates of cardiac/respiratory problems, anemia, PROM, and endometritis but were less prone to develop pre-eclampsia. Prematurity and low Apgar scores were significantly more frequent in the infants of underweight women. There was no difference in the frequency of IUGR and in perinatal mortality rates. The mean birth weight of the infants of underweight women was 231 grams less than that of infants of control subjects. Underweight women, particularly if they were anemic, had a higher incidence of low-birth-weight infants despite adequate weight gain. AGA infants of underweight women were more likely to be below the twenty-fifth percentile for weight correlated with length by 12 months of age."}
{"id": "PMID:484617", "title": "Effect of maternal nutritional status and infant supplementation during lactation on postpartum amenorrhea.", "content": "This paper explores the effects of maternal nutritional status and food supplementation ingested by the infant on the duration of postpartum amenorrhea. A significant negative association was found between the nutritional status of the mother during the third trimester of pregnancy and the length of postpartum amenorrhea. A negative association was also found between infant supplementation and duration of postpartum amenorrhea. Each of these associations remained significant after controlling for the other, suggesting that both factors independently affect the length of postpartum amenorrhea.", "contents": "Effect of maternal nutritional status and infant supplementation during lactation on postpartum amenorrhea. This paper explores the effects of maternal nutritional status and food supplementation ingested by the infant on the duration of postpartum amenorrhea. A significant negative association was found between the nutritional status of the mother during the third trimester of pregnancy and the length of postpartum amenorrhea. A negative association was also found between infant supplementation and duration of postpartum amenorrhea. Each of these associations remained significant after controlling for the other, suggesting that both factors independently affect the length of postpartum amenorrhea."}
{"id": "PMID:484618", "title": "Studies of colloid osmotic pressure in pregnancy-induced hypertension.", "content": "Pulmonary edema, although infrequently encountered, may cause death in severe cases of PIH. The mechanisms that account for edema in these instances are unclear. With a transducer-membrane system to directly measure serum COP, 22 patients with PIH were compared to 23 normal pregnant patients at term. In the PIH group the antepartum and postpartum COP values were significantly lower (17.9 and 13.7 mm Hg, respectively) than comparable values for the control group (22.0 and 17.2 mm Hg, respectively). Two patients in the PIH group in whom the antepartum COP values were 13.1 and 14.7 mm Hg exhibited clinical and radiographic evidence of pulmonary edema. Pulmonary artery wedge pressure (PAW) recorded in one patient with pulmonary edema was only moderately elevated to 18 mm Hg. We believe that serial COP determinations combined with pulmonary artery pressure monitoring may be helpful to guide fluid therapy in critically ill patients with PIH.", "contents": "Studies of colloid osmotic pressure in pregnancy-induced hypertension. Pulmonary edema, although infrequently encountered, may cause death in severe cases of PIH. The mechanisms that account for edema in these instances are unclear. With a transducer-membrane system to directly measure serum COP, 22 patients with PIH were compared to 23 normal pregnant patients at term. In the PIH group the antepartum and postpartum COP values were significantly lower (17.9 and 13.7 mm Hg, respectively) than comparable values for the control group (22.0 and 17.2 mm Hg, respectively). Two patients in the PIH group in whom the antepartum COP values were 13.1 and 14.7 mm Hg exhibited clinical and radiographic evidence of pulmonary edema. Pulmonary artery wedge pressure (PAW) recorded in one patient with pulmonary edema was only moderately elevated to 18 mm Hg. We believe that serial COP determinations combined with pulmonary artery pressure monitoring may be helpful to guide fluid therapy in critically ill patients with PIH."}
{"id": "PMID:484619", "title": "Fetal risk in delivery with the Shute parallel forceps: analysis of 1,503 forceps deliveries.", "content": "A total of 1,503 vaginal deliveries with the Shute parallel forceps during a 10 year period are analyzed as to the risk of instrumental damage. In this series, 35 infants died intrapartum or neonatally. Eliminating all deaths from unrelated causes, three remain in which the forceps could possibly have been implicated. Investigation of these, however, revealed in each case the presence of other concomitant and potentially lethal factors, none of which could be completely ruled out as the primary cause of fetal death. Each of the three cases is discussed in detail. We conclude from our series that the Shute forceps is useful in the delivery of premature infants, but should be employed for this maneuver only by very experienced operators. In these cases, midforceps should be performed only for critical indications. The risk of damage with parallel forceps deliveries from the pelvic floor is minimal if decision for operation is based on cardiotocographic criteria, and under favorable degrees of oxygenation. In the delivery of the immature infants, the parallel forceps can, in fact, hardly be superseded by any other instrument because of its unique controlled protection of the fragile fetal head from even the pressures of the birth canal. Delivery with the Shute forceps can be performed effectively under pudendal block or local infiltration anesthesia.", "contents": "Fetal risk in delivery with the Shute parallel forceps: analysis of 1,503 forceps deliveries. A total of 1,503 vaginal deliveries with the Shute parallel forceps during a 10 year period are analyzed as to the risk of instrumental damage. In this series, 35 infants died intrapartum or neonatally. Eliminating all deaths from unrelated causes, three remain in which the forceps could possibly have been implicated. Investigation of these, however, revealed in each case the presence of other concomitant and potentially lethal factors, none of which could be completely ruled out as the primary cause of fetal death. Each of the three cases is discussed in detail. We conclude from our series that the Shute forceps is useful in the delivery of premature infants, but should be employed for this maneuver only by very experienced operators. In these cases, midforceps should be performed only for critical indications. The risk of damage with parallel forceps deliveries from the pelvic floor is minimal if decision for operation is based on cardiotocographic criteria, and under favorable degrees of oxygenation. In the delivery of the immature infants, the parallel forceps can, in fact, hardly be superseded by any other instrument because of its unique controlled protection of the fragile fetal head from even the pressures of the birth canal. Delivery with the Shute forceps can be performed effectively under pudendal block or local infiltration anesthesia."}
{"id": "PMID:484620", "title": "Myoendothelial and placental blood flow responses to ritodrine infusion in the guinea pig.", "content": "The effect of ritodrine hydrochloride on uterine blood flow was investigated in near-term guinea pigs. The infusion of ritodrine in doses sufficient to inhibit uterine activity provoked tachycardia, and the cardiac output tended to rise. The percentage of cardiac output reaching the gravid uterus did not alter during the infusion of 12 micrograms per minute of ritodrine but decreased from 12% to 10% when the infusion rate was 120 micrograms per minute. There was an increase in the myoendothelial fraction of cardiac output in both instances, whereas the placental fraction decreased at the higher rate of infusion. Perfusion of the myoendothelial tissue improved during the infusion of 12 or 120 micrograms per minute of ritodrine, increasing by 25% and 18% respectively. No significant alteration occurred in the perfusion of the placental tissue.", "contents": "Myoendothelial and placental blood flow responses to ritodrine infusion in the guinea pig. The effect of ritodrine hydrochloride on uterine blood flow was investigated in near-term guinea pigs. The infusion of ritodrine in doses sufficient to inhibit uterine activity provoked tachycardia, and the cardiac output tended to rise. The percentage of cardiac output reaching the gravid uterus did not alter during the infusion of 12 micrograms per minute of ritodrine but decreased from 12% to 10% when the infusion rate was 120 micrograms per minute. There was an increase in the myoendothelial fraction of cardiac output in both instances, whereas the placental fraction decreased at the higher rate of infusion. Perfusion of the myoendothelial tissue improved during the infusion of 12 or 120 micrograms per minute of ritodrine, increasing by 25% and 18% respectively. No significant alteration occurred in the perfusion of the placental tissue."}
{"id": "PMID:484621", "title": "Absence of human placental lactogen in an otherwise uneventful pregnancy.", "content": "Human placental lactogen (hPL) is commonly used in surveying the placental function during normal and pathologic pregnancies. This report describes a pregnancy where hPL could not be found in maternal serum or placental tissue. The pregnancy was in all other respects completely normal, ending with the birth of a normal baby. Some possible reasons and consequences of this unique event are discussed.", "contents": "Absence of human placental lactogen in an otherwise uneventful pregnancy. Human placental lactogen (hPL) is commonly used in surveying the placental function during normal and pathologic pregnancies. This report describes a pregnancy where hPL could not be found in maternal serum or placental tissue. The pregnancy was in all other respects completely normal, ending with the birth of a normal baby. Some possible reasons and consequences of this unique event are discussed."}
{"id": "PMID:484622", "title": "Saline-instillation abortion with laminaria and megadose oxytocin.", "content": "This study examines the effect of laminaria and oxytocin in 638 saline instillation abortions. Laminaria were inserted into the cervical canal in the physician's office 2 to 3 hours before admission to hospital for instillation. Pitocin was given intravenously at a rate of 417 to 555 mu/min starting 2 hours after instillation. Pitocin was given intravenously at a rate of 417 to 555 mu/min starting 2 hours after instillation. Center for Disease Control (CDC) criteria were used to define complications in the present series. Complication rates compared favorably to those in a national CDC study of traditional saline procedures. Instillation-abortion times averaged 12.5 hours in the present series, about half the time previously reported for saline abortion. If confirmed by other studies, laminaria and oxytocin will be beneficial adjuncts for managing patients aborted by saline instillation.", "contents": "Saline-instillation abortion with laminaria and megadose oxytocin. This study examines the effect of laminaria and oxytocin in 638 saline instillation abortions. Laminaria were inserted into the cervical canal in the physician's office 2 to 3 hours before admission to hospital for instillation. Pitocin was given intravenously at a rate of 417 to 555 mu/min starting 2 hours after instillation. Pitocin was given intravenously at a rate of 417 to 555 mu/min starting 2 hours after instillation. Center for Disease Control (CDC) criteria were used to define complications in the present series. Complication rates compared favorably to those in a national CDC study of traditional saline procedures. Instillation-abortion times averaged 12.5 hours in the present series, about half the time previously reported for saline abortion. If confirmed by other studies, laminaria and oxytocin will be beneficial adjuncts for managing patients aborted by saline instillation."}
{"id": "PMID:484623", "title": "Fetal growth and placental function assessed by urinary estriol excretion before the onset of pre-eclampsia.", "content": "In a series of 1,316 patients with pre-eclampsia 744 had urinary estriol excretion measured before and 366 after the onset of clinical signs of the disease. Low estriol excretion had a highly significant association with fetal growth retardation and perinatal death both before and after the onset of clinical signs (p less than 0.001). As assessed by the incidences of low estriol excretion, fetal growth retardation, and perinatal wastage, pre-eclampsia of early onset (before 37 weeks) was a malignant disease in comparison with pre-eclampsia of late onset (after 37 weeks). Patients destined to develop early-onset pre-eclampsia had a high incidence of subnormal estriol excretion (25.4%; p less than 0.001). Although further deterioration of placental function occurred after the onset of clinical signs (41.3%; p less than 0.01), fetal growth and prognosis were already determined.", "contents": "Fetal growth and placental function assessed by urinary estriol excretion before the onset of pre-eclampsia. In a series of 1,316 patients with pre-eclampsia 744 had urinary estriol excretion measured before and 366 after the onset of clinical signs of the disease. Low estriol excretion had a highly significant association with fetal growth retardation and perinatal death both before and after the onset of clinical signs (p less than 0.001). As assessed by the incidences of low estriol excretion, fetal growth retardation, and perinatal wastage, pre-eclampsia of early onset (before 37 weeks) was a malignant disease in comparison with pre-eclampsia of late onset (after 37 weeks). Patients destined to develop early-onset pre-eclampsia had a high incidence of subnormal estriol excretion (25.4%; p less than 0.001). Although further deterioration of placental function occurred after the onset of clinical signs (41.3%; p less than 0.01), fetal growth and prognosis were already determined."}
{"id": "PMID:484624", "title": "Cigarette smoking as an etiologic factor in cleft lip and palate.", "content": "A case-control study has been made on smoking habits in women who, during 1975, gave birth to infants with closure defects of the central nervous system (ASB) or with cleft lip or cleft palate (CLP). For each case, two control subjects with nonmalformed infants were selected and matched for delivery unit, time of delivery, maternal age, and maternal parity. Smoking habits were routinely included in hospital records at first visit to a maternity health clinic during pregnancy. Data were studied for 66 cases of CLP, 66 cases of ASB, and 261 control subjects. Significantly more women who had infants with CLP smoked than did control women, but women with ASB infants showed a normal smoking pattern. Drug use did not explain the findings. It is suggested that maternal smoking is one of many factors of importance in the etiology of cleft lip and cleft palate in humans.", "contents": "Cigarette smoking as an etiologic factor in cleft lip and palate. A case-control study has been made on smoking habits in women who, during 1975, gave birth to infants with closure defects of the central nervous system (ASB) or with cleft lip or cleft palate (CLP). For each case, two control subjects with nonmalformed infants were selected and matched for delivery unit, time of delivery, maternal age, and maternal parity. Smoking habits were routinely included in hospital records at first visit to a maternity health clinic during pregnancy. Data were studied for 66 cases of CLP, 66 cases of ASB, and 261 control subjects. Significantly more women who had infants with CLP smoked than did control women, but women with ASB infants showed a normal smoking pattern. Drug use did not explain the findings. It is suggested that maternal smoking is one of many factors of importance in the etiology of cleft lip and cleft palate in humans."}
{"id": "PMID:484625", "title": "R--pulse wave timing: a technique for continuous cardiovascular monitoring in obstetrics--preliminary report.", "content": "A simple, noninvasive technique for continuous cardiovascular monitoring in obstetrics using R--pulse wave timing is described. This preliminary study suggests that different maternal heart rate/R pulse patterns are found in different clinical circumstances. A significant decrease in the R pulse interval occurred in the third trimester in both normal and diabetic women in the left lateral position when compared to the supine position. The R pulse intervals were all prolonged in the standing position in both gravid and nongravid women. During labor R pulse interval usually shortened during uterine contraction. Prolongation of R pulse interval was observed during thiopental injection, hypotension, and excessive bleeding at cesarean section. Exercise testing in healthy male subjects suggested that the change in R pulse interval becomes constant when the oxygen consumption rate reaches a plateau at 100% maximal work load. Further extensive clinical study appears worthwhile since it may add another dimension to cardiovascular monitoring and give an early indication of cardiovascular reserve.", "contents": "R--pulse wave timing: a technique for continuous cardiovascular monitoring in obstetrics--preliminary report. A simple, noninvasive technique for continuous cardiovascular monitoring in obstetrics using R--pulse wave timing is described. This preliminary study suggests that different maternal heart rate/R pulse patterns are found in different clinical circumstances. A significant decrease in the R pulse interval occurred in the third trimester in both normal and diabetic women in the left lateral position when compared to the supine position. The R pulse intervals were all prolonged in the standing position in both gravid and nongravid women. During labor R pulse interval usually shortened during uterine contraction. Prolongation of R pulse interval was observed during thiopental injection, hypotension, and excessive bleeding at cesarean section. Exercise testing in healthy male subjects suggested that the change in R pulse interval becomes constant when the oxygen consumption rate reaches a plateau at 100% maximal work load. Further extensive clinical study appears worthwhile since it may add another dimension to cardiovascular monitoring and give an early indication of cardiovascular reserve."}
{"id": "PMID:484626", "title": "A study of variable decelerations in association with other heart rate patterns during monitored labor.", "content": "A review of 1,011 consecutive intrapartum heart rate tracings yielded 37.3% with some degree of variable deceleration pattern. No differences in Apgar score distribution were observed in the presence of uncomplicated variable deceleration pattern when compared to those tracings marked normal. However, the presence of variable decelerations in association with other heart rate patterns resulted in lower mean Apgar scores at 1 and 5 minutes, which were significantly different from those of the fetal heart rate (FHR) normal group. Mean Apgar scores at 1 and 5 minutes were significantly different from normal when variable decelerations were noted in the presence of tachycardia and loss of variability. Mean Apgar scores were lower when bradycardia (prolonged episodes of heart rate less than 120 bpm) was present in the record when compared to normal, but the presence of variable decelerations with bradycardia did not result in different mean scores. The presence of baseline changes with loss of variability and variable decelerations appeared to result in the lowest mean scores. When bradycardia or tachycardia occurred in exclusive association with variable decelerations, the percentage of depressed newborn infants was relatively high.", "contents": "A study of variable decelerations in association with other heart rate patterns during monitored labor. A review of 1,011 consecutive intrapartum heart rate tracings yielded 37.3% with some degree of variable deceleration pattern. No differences in Apgar score distribution were observed in the presence of uncomplicated variable deceleration pattern when compared to those tracings marked normal. However, the presence of variable decelerations in association with other heart rate patterns resulted in lower mean Apgar scores at 1 and 5 minutes, which were significantly different from those of the fetal heart rate (FHR) normal group. Mean Apgar scores at 1 and 5 minutes were significantly different from normal when variable decelerations were noted in the presence of tachycardia and loss of variability. Mean Apgar scores were lower when bradycardia (prolonged episodes of heart rate less than 120 bpm) was present in the record when compared to normal, but the presence of variable decelerations with bradycardia did not result in different mean scores. The presence of baseline changes with loss of variability and variable decelerations appeared to result in the lowest mean scores. When bradycardia or tachycardia occurred in exclusive association with variable decelerations, the percentage of depressed newborn infants was relatively high."}
{"id": "PMID:484627", "title": "Labor decreases the lung water content of newborn rabbits.", "content": "To test the prevailing concept that drainage of fetal lung liquid begins after birth, we measured extravascular lung water content of 47 fetal rabbits born at term gestation, with or without prior labor. Rabbits born after labor, either vaginally or operatively, had less water in their lungs than those delivered by cesarean section without preceding labor; there was no difference in lung water content between rabbits born vaginally or operatively after labor. These results suggest that reduction in the volume of fetal lung liquid in rabbits normally begins before birth and depends on the experience of labor, not the mode of delivery.", "contents": "Labor decreases the lung water content of newborn rabbits. To test the prevailing concept that drainage of fetal lung liquid begins after birth, we measured extravascular lung water content of 47 fetal rabbits born at term gestation, with or without prior labor. Rabbits born after labor, either vaginally or operatively, had less water in their lungs than those delivered by cesarean section without preceding labor; there was no difference in lung water content between rabbits born vaginally or operatively after labor. These results suggest that reduction in the volume of fetal lung liquid in rabbits normally begins before birth and depends on the experience of labor, not the mode of delivery."}
{"id": "PMID:484628", "title": "Changes of lipid peroxidation and superoxide dismutase activity in the human placenta.", "content": "The oxygen radical may induce peroxidation of unsaturated fatty acids in vivo. We studied changes in superoxide dismutase (SOD) activity and lipid peroxidation using human placental tissue. Lipid peroxidation was marked in early stages (2 to 4 months) of gestation but decreased with growth and was very small by the end of pregnancy. The SOD activity of placental tissue in early gestation was approximately 250 to 500 U per gram of wet weight. The activity increased with growth of the placenta and reached a level of 400 to 1,500 U per gram of wet weight by the end of gestation. These results for SOD activity suggest that the oxygen requirement in the placenta at early stages of gestation is low compared with that at the end of gestation.", "contents": "Changes of lipid peroxidation and superoxide dismutase activity in the human placenta. The oxygen radical may induce peroxidation of unsaturated fatty acids in vivo. We studied changes in superoxide dismutase (SOD) activity and lipid peroxidation using human placental tissue. Lipid peroxidation was marked in early stages (2 to 4 months) of gestation but decreased with growth and was very small by the end of pregnancy. The SOD activity of placental tissue in early gestation was approximately 250 to 500 U per gram of wet weight. The activity increased with growth of the placenta and reached a level of 400 to 1,500 U per gram of wet weight by the end of gestation. These results for SOD activity suggest that the oxygen requirement in the placenta at early stages of gestation is low compared with that at the end of gestation."}
{"id": "PMID:484629", "title": "Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood.", "content": "Concentrations of a peroxidation product (malondialdehyde), fluorescent chromophores, lipofuscin-like fluorescent products, superoxide dismutase, catalase, glutathione peroxidase, and vitamin E in the maternal blood and the cord blood were determined and the results obtained were related to the estimation of lipid peroxidation and protective mechanism against uncontrolled oxidative processes in late pregnancy. Serum levels of fluorescent products were higher in the maternal blood than in the cord blood, indicating less frequent lipid peroxidation in the fetus than in the mother. In support of this assumption, the three protective enzymes and vitamin E were present in relatively lower concentrations in the cord blood. Sudden exposure of the newborn infant to a normobaric atmosphere after beginning breathing seems, therefore, to cause oxidation of red blood cell membrane, denaturation of the membrane, inducing hemoglobin breakdown, and consequently hemolysis.", "contents": "Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Concentrations of a peroxidation product (malondialdehyde), fluorescent chromophores, lipofuscin-like fluorescent products, superoxide dismutase, catalase, glutathione peroxidase, and vitamin E in the maternal blood and the cord blood were determined and the results obtained were related to the estimation of lipid peroxidation and protective mechanism against uncontrolled oxidative processes in late pregnancy. Serum levels of fluorescent products were higher in the maternal blood than in the cord blood, indicating less frequent lipid peroxidation in the fetus than in the mother. In support of this assumption, the three protective enzymes and vitamin E were present in relatively lower concentrations in the cord blood. Sudden exposure of the newborn infant to a normobaric atmosphere after beginning breathing seems, therefore, to cause oxidation of red blood cell membrane, denaturation of the membrane, inducing hemoglobin breakdown, and consequently hemolysis."}
{"id": "PMID:484630", "title": "Postirradiation squamous cell carcinoma in situ of the vagina: treatment by topical 20 percent 5-fluorouracil cream.", "content": "Eight patients with squamous cell carcinoma in situ of the vagina were treated with monthly 5 day courses of 20% 5-fluorouracil cream applied to the vagina. Seven (87.5%) of the women had an initial complete response; however, three subsequently developed recurrence after the treatment was stopped. They were retreated, and two of these three patients achieved a subsequent complete response. The overall response to therapy was 75% (6/8) with six women off therapy from 1.1 to 6.9 years. Total follow-up of all patients was 3.1 to 6.9 years with no patients having developed invasive carcinoma. Because of the high success rate with no associated serous sequelae, this is believed to be a satisfactory method of therapy for women with squamous cell carcinoma in situ of the vagina occurring after pelvic irradiation.", "contents": "Postirradiation squamous cell carcinoma in situ of the vagina: treatment by topical 20 percent 5-fluorouracil cream. Eight patients with squamous cell carcinoma in situ of the vagina were treated with monthly 5 day courses of 20% 5-fluorouracil cream applied to the vagina. Seven (87.5%) of the women had an initial complete response; however, three subsequently developed recurrence after the treatment was stopped. They were retreated, and two of these three patients achieved a subsequent complete response. The overall response to therapy was 75% (6/8) with six women off therapy from 1.1 to 6.9 years. Total follow-up of all patients was 3.1 to 6.9 years with no patients having developed invasive carcinoma. Because of the high success rate with no associated serous sequelae, this is believed to be a satisfactory method of therapy for women with squamous cell carcinoma in situ of the vagina occurring after pelvic irradiation."}
{"id": "PMID:484631", "title": "Computerized tomography applied to gynecologic oncology.", "content": "Forty patients on the gynecologic oncology service at the University of North Carolina were evaluated with CT scans. Accuracy and clinical benefit of these scans were compared to those of manual clinical examinations. The CT scans were generally superior and had fewer (9) verified errors in the regions of the pelvic wall and para-aortic area than did the manual examinations (17). Both the CT and bimanual examinations had the same number of verified errors (3) in the central pelvic region. The authors found the CT scans to be beneficial in evaluation of pelvic wall and para-aortic regions for treatment planning of either primary or recurrent cancer.", "contents": "Computerized tomography applied to gynecologic oncology. Forty patients on the gynecologic oncology service at the University of North Carolina were evaluated with CT scans. Accuracy and clinical benefit of these scans were compared to those of manual clinical examinations. The CT scans were generally superior and had fewer (9) verified errors in the regions of the pelvic wall and para-aortic area than did the manual examinations (17). Both the CT and bimanual examinations had the same number of verified errors (3) in the central pelvic region. The authors found the CT scans to be beneficial in evaluation of pelvic wall and para-aortic regions for treatment planning of either primary or recurrent cancer."}
{"id": "PMID:484632", "title": "The influence of acetylsalicylic acid and paracetamol on menstrual blood loss in women with and without an intrauterine contraceptive device.", "content": "The influence of ASA and paracetamol on menstural blood loss and on some hematologic parameters was investigated in 23 women without an IUD and 10 women with an IUD. Neither in women with normal nor in women with small defects in the hemostatic mechanism were statistically significant increases in menstrual blood losses observed during treatment with ASA or paracetamol when compared to placebo. There was no linear correlation between bleeding time and basal menstrual blood loss or between the blood losses induced by ASA and paracetamol and the bleeding time.", "contents": "The influence of acetylsalicylic acid and paracetamol on menstrual blood loss in women with and without an intrauterine contraceptive device. The influence of ASA and paracetamol on menstural blood loss and on some hematologic parameters was investigated in 23 women without an IUD and 10 women with an IUD. Neither in women with normal nor in women with small defects in the hemostatic mechanism were statistically significant increases in menstrual blood losses observed during treatment with ASA or paracetamol when compared to placebo. There was no linear correlation between bleeding time and basal menstrual blood loss or between the blood losses induced by ASA and paracetamol and the bleeding time."}
{"id": "PMID:484633", "title": "The intrauterine contraceptive device and acute salpingitis: a multifactor analysis.", "content": "From an indigent population hospitalized with acute salpingitis, 163 patients were compared with 222 control patients from the minor trauma section of the emergency room. Four factors were significantly different between cases and controls: race, type of contraception, number of sex partners, and previous history of salpingitis. A discriminate analysis based on a linear logistic equation demonstrated that each risk factor was independent rather than a reflection of another risk factor. Thus factors associated with race, IUD use, multiple sexual partners, and previous salpingitis increase the risk of salpingitis in the population studied.", "contents": "The intrauterine contraceptive device and acute salpingitis: a multifactor analysis. From an indigent population hospitalized with acute salpingitis, 163 patients were compared with 222 control patients from the minor trauma section of the emergency room. Four factors were significantly different between cases and controls: race, type of contraception, number of sex partners, and previous history of salpingitis. A discriminate analysis based on a linear logistic equation demonstrated that each risk factor was independent rather than a reflection of another risk factor. Thus factors associated with race, IUD use, multiple sexual partners, and previous salpingitis increase the risk of salpingitis in the population studied."}
{"id": "PMID:484634", "title": "Pharmacokinetics of norethindrone acetate in women.", "content": "The plasma half-life, MCR and plasma metabolite levels at various time intervals have been studied in six women after an intravenous injection of 3H-norethindrone acetate. The disappearance curve due to norethindrone acetate showed an initial rapid disappearance of 3H with an average half-life of 7.5 minutes and a subsequent slow disapperance with a half-life of 51.5 hours. Norethindrone acetate was cleared from the plasma with an average MCR of 495 L/day. Norethindrone acetate is rapidly metabolised after an intravenous injection. Norethindrone, the main metabolite, disappears from the plasma with an average half-life of 34.8 hours. Norethindrone maintains a high level compared with norethindrone acetate at all time intervals up to 24 hours and an equilibrium is reached between the two at 24 to 48 hours.", "contents": "Pharmacokinetics of norethindrone acetate in women. The plasma half-life, MCR and plasma metabolite levels at various time intervals have been studied in six women after an intravenous injection of 3H-norethindrone acetate. The disappearance curve due to norethindrone acetate showed an initial rapid disappearance of 3H with an average half-life of 7.5 minutes and a subsequent slow disapperance with a half-life of 51.5 hours. Norethindrone acetate was cleared from the plasma with an average MCR of 495 L/day. Norethindrone acetate is rapidly metabolised after an intravenous injection. Norethindrone, the main metabolite, disappears from the plasma with an average half-life of 34.8 hours. Norethindrone maintains a high level compared with norethindrone acetate at all time intervals up to 24 hours and an equilibrium is reached between the two at 24 to 48 hours."}
{"id": "PMID:484635", "title": "delta 4-3-Ketosteroid 5 alpha-oxidoreductase in human uterine leiomyoma.", "content": "Homogenates of human endometrial, myometrial, and leiomyoma tissues were incubated with (1,2,6,7-3H)-testosterone, and 3H-dihydrotestosterone was subsequently identified, indicating the presence of the enzyme delta 4-3-Ketosteroid 5 alpha-oxidoreductase. The enzyme activity of leiomyoma was higher than that of endometrial and myometrial tissue, while endometrial activity was higher than myometrial activity. The results raise the possibility that 5 alpha-reduced androgens may play a role in the pathophysiology of uterine leiomyoma.", "contents": "delta 4-3-Ketosteroid 5 alpha-oxidoreductase in human uterine leiomyoma. Homogenates of human endometrial, myometrial, and leiomyoma tissues were incubated with (1,2,6,7-3H)-testosterone, and 3H-dihydrotestosterone was subsequently identified, indicating the presence of the enzyme delta 4-3-Ketosteroid 5 alpha-oxidoreductase. The enzyme activity of leiomyoma was higher than that of endometrial and myometrial tissue, while endometrial activity was higher than myometrial activity. The results raise the possibility that 5 alpha-reduced androgens may play a role in the pathophysiology of uterine leiomyoma."}
{"id": "PMID:484640", "title": "Modern indications for cesarean section.", "content": "Total cesarean section rates and rates of various indications for primary cesarean section were reviewed for four yearly periods during the past decade. The time periods studied were chosen to follow the introduction of new obstetric practices and technologic advances in monitoring fetal condition. The total cesarean section rate increased from 6.8% to 17.1% during this time. The most common indication for primary cesarean section was cephalopelvic disproportion, which represented approximately 40% of cases during each study period. Primary cesarean section for fetal distress increased to 28.2% but has decreased over the past 2 years to a present rate of 11.7%. Primary cesarean section for breech presentation increased from 12.3% to 21%. From the perspective of this review a total cesarean section rate of 15% is predicted for the future.", "contents": "Modern indications for cesarean section. Total cesarean section rates and rates of various indications for primary cesarean section were reviewed for four yearly periods during the past decade. The time periods studied were chosen to follow the introduction of new obstetric practices and technologic advances in monitoring fetal condition. The total cesarean section rate increased from 6.8% to 17.1% during this time. The most common indication for primary cesarean section was cephalopelvic disproportion, which represented approximately 40% of cases during each study period. Primary cesarean section for fetal distress increased to 28.2% but has decreased over the past 2 years to a present rate of 11.7%. Primary cesarean section for breech presentation increased from 12.3% to 21%. From the perspective of this review a total cesarean section rate of 15% is predicted for the future."}
{"id": "PMID:484641", "title": "Surgical treatment of stages IB and IIA invasive carcinoma of the cervix by radical abdominal hysterectomy.", "content": "From 1963 to 1977, 349 radical abdominal hysterectomies with bilateral pelvic lymphadenectomy were performed for Stage IB (331 patients) and Stage IIA (18 patients) cervical cancer at the New York Medical College, with no operative deaths. Definitive diagnosis was obtained from the biopsy specimen in 281 patients. Twenty-nine patients were pregnant when the diagnosis was established. The average operating time was 4 hours and 48 minutes with an average blood loss of 900 ml. Eleven fistulas were noted: ureterovaginal, 7; vesicovaginal, 3; rectovaginal, 1. Since 1972, there have been no fistulas in 130 radical hysterectomies. Metastatic carcinoma of the regional lymph nodes was discovered in 27 patients for an incidence of 7.7%. Postoperative total pelvic external irradiation was utilized in 40 patients (27 with positive nodes, 10 with microscopic carcinoma in vascular channels, and an additional three patients with an inadequate vaginal extirpation margin). A total of 62% of those patients with poor prognostic criteria receiving postoperative irradiation are alive and well. Two hundred nineteen patients have been followed up for at least 5 years and the survival rate was 90%. Our data support the view that radical abdominal hysterectomy with bilateral pelvic lymphadenectomy is the treatment of choice for patients with Stages IB and IIA cervical cancer in the nonpregnant state, unless there are major medical contraindications.", "contents": "Surgical treatment of stages IB and IIA invasive carcinoma of the cervix by radical abdominal hysterectomy. From 1963 to 1977, 349 radical abdominal hysterectomies with bilateral pelvic lymphadenectomy were performed for Stage IB (331 patients) and Stage IIA (18 patients) cervical cancer at the New York Medical College, with no operative deaths. Definitive diagnosis was obtained from the biopsy specimen in 281 patients. Twenty-nine patients were pregnant when the diagnosis was established. The average operating time was 4 hours and 48 minutes with an average blood loss of 900 ml. Eleven fistulas were noted: ureterovaginal, 7; vesicovaginal, 3; rectovaginal, 1. Since 1972, there have been no fistulas in 130 radical hysterectomies. Metastatic carcinoma of the regional lymph nodes was discovered in 27 patients for an incidence of 7.7%. Postoperative total pelvic external irradiation was utilized in 40 patients (27 with positive nodes, 10 with microscopic carcinoma in vascular channels, and an additional three patients with an inadequate vaginal extirpation margin). A total of 62% of those patients with poor prognostic criteria receiving postoperative irradiation are alive and well. Two hundred nineteen patients have been followed up for at least 5 years and the survival rate was 90%. Our data support the view that radical abdominal hysterectomy with bilateral pelvic lymphadenectomy is the treatment of choice for patients with Stages IB and IIA cervical cancer in the nonpregnant state, unless there are major medical contraindications."}
{"id": "PMID:484642", "title": "Therapy of endodermal sinus tumor of the ovary.", "content": "Endodermal sinus tumor of the ovary can be differentiated histologically and immunohistochemically from ovarian embryonal cell carcinoma. A case report of a patient with endodermal sinus tumor is presented in which a long-term remission was achieved by unilateral adnexectomy and combination chemotherapy. Review of the current literature indicates that tumor removal followed by combination chemotherapy with vincristine, actinomycin D, and cyclophosphamide is the most effective method of therapy for this highly malignant ovarian neoplasm. The addition of hysterectomy with contralateral ovariectomy or radiation therapy does not appear to significantly improve the survival of patients with this tumor. Serial plasma determinations of alpha fetoprotein provide biochemical monitoring of response to therapy and may be useful in predicting occult tumor recurrence.", "contents": "Therapy of endodermal sinus tumor of the ovary. Endodermal sinus tumor of the ovary can be differentiated histologically and immunohistochemically from ovarian embryonal cell carcinoma. A case report of a patient with endodermal sinus tumor is presented in which a long-term remission was achieved by unilateral adnexectomy and combination chemotherapy. Review of the current literature indicates that tumor removal followed by combination chemotherapy with vincristine, actinomycin D, and cyclophosphamide is the most effective method of therapy for this highly malignant ovarian neoplasm. The addition of hysterectomy with contralateral ovariectomy or radiation therapy does not appear to significantly improve the survival of patients with this tumor. Serial plasma determinations of alpha fetoprotein provide biochemical monitoring of response to therapy and may be useful in predicting occult tumor recurrence."}
{"id": "PMID:484643", "title": "Gestational trophoblastic disease within an elective abortion population.", "content": "Gestational trophoblastic disease, most commonly hydatidiform mole, is an unusual condition within the United States. The incidence of hydatidiform mole has been reported to vary from 1 in 1,200 to 1 in 2,000 pregnancies. This report described eight cases of hydatidiform mole among 4,829 patients presenting for elective first-trimester abortion. Factors which might account for the frequency of hydatidiform mole of 1 in 600 are discussed. Although the clinical course of patients with hydatidiform mole appears benign- gross examination of tissue obtained at suction curettage and the liberal use of histologic evaluation in questionable cases is required to make the diagnosis.", "contents": "Gestational trophoblastic disease within an elective abortion population. Gestational trophoblastic disease, most commonly hydatidiform mole, is an unusual condition within the United States. The incidence of hydatidiform mole has been reported to vary from 1 in 1,200 to 1 in 2,000 pregnancies. This report described eight cases of hydatidiform mole among 4,829 patients presenting for elective first-trimester abortion. Factors which might account for the frequency of hydatidiform mole of 1 in 600 are discussed. Although the clinical course of patients with hydatidiform mole appears benign- gross examination of tissue obtained at suction curettage and the liberal use of histologic evaluation in questionable cases is required to make the diagnosis."}
{"id": "PMID:484644", "title": "Anaprox in dysmenorrhea: reduction of pain and intrauterine pressure.", "content": "In a double-blind parallel trial, 24 dysmenorrheic women received a single dose of Anaprox (1,100 mg) or placebo. Over the next 2 hours, pain intensity was scored and intrauterine pressure was measured using an immobilized microballoon. At the end of 2 hours, all 11 patients given Anaprox (but only three of the 13 given placebo) experienced complete pain relief (p = 0.0004). The resting intrauterine pressure (IUP) decreased from a mean of 51.4 to 26.8 mm Hg in the Anaprox-treated group, while in the placebo group the mean resting IUP values remained essentially unchanged ( drop from 55.4 to 51.9 mm Hg was observed). This difference between the two treatment groups was statistically significant in favor of Anaprox (p = 0.03). Several patients from each group were given 0.2 mg of ergonovine by intramuscular injection following the 2 hour trial. In both groups, the resting IUP increased within 30 minutes; the corresponding increase in pain intensity was more pronounced, however, in the placebo group. These results support the premise that a decrease in resting IUP is directly linked to the pain-relieving effects of Anaprox.", "contents": "Anaprox in dysmenorrhea: reduction of pain and intrauterine pressure. In a double-blind parallel trial, 24 dysmenorrheic women received a single dose of Anaprox (1,100 mg) or placebo. Over the next 2 hours, pain intensity was scored and intrauterine pressure was measured using an immobilized microballoon. At the end of 2 hours, all 11 patients given Anaprox (but only three of the 13 given placebo) experienced complete pain relief (p = 0.0004). The resting intrauterine pressure (IUP) decreased from a mean of 51.4 to 26.8 mm Hg in the Anaprox-treated group, while in the placebo group the mean resting IUP values remained essentially unchanged ( drop from 55.4 to 51.9 mm Hg was observed). This difference between the two treatment groups was statistically significant in favor of Anaprox (p = 0.03). Several patients from each group were given 0.2 mg of ergonovine by intramuscular injection following the 2 hour trial. In both groups, the resting IUP increased within 30 minutes; the corresponding increase in pain intensity was more pronounced, however, in the placebo group. These results support the premise that a decrease in resting IUP is directly linked to the pain-relieving effects of Anaprox."}
{"id": "PMID:484645", "title": "Systemic lupus erythematosus in pregnancy.", "content": "Most literature on pregnancies in patients with systemic lupus erythematosus (SLE) is retrospective and selective. This report is a detailed, prospective analysis of 13 pregnancies in eight women with SLE. Pregnancy was best tolerated by mothers without significant nephropathy or cardiopathy who had been in clinical remission for more than three months prior to conception. Management was aided by serial evaluation of complement (C3 and C4) levels and careful supervision of immunosuppressive therapy when indicated. Although fetal status was closely monitored, premature deliveries and spontaneous abortions occurred frequently. No malformations or adverse sequelae were noted in surviving infants exposed to immunosuppressive agents during gestation.", "contents": "Systemic lupus erythematosus in pregnancy. Most literature on pregnancies in patients with systemic lupus erythematosus (SLE) is retrospective and selective. This report is a detailed, prospective analysis of 13 pregnancies in eight women with SLE. Pregnancy was best tolerated by mothers without significant nephropathy or cardiopathy who had been in clinical remission for more than three months prior to conception. Management was aided by serial evaluation of complement (C3 and C4) levels and careful supervision of immunosuppressive therapy when indicated. Although fetal status was closely monitored, premature deliveries and spontaneous abortions occurred frequently. No malformations or adverse sequelae were noted in surviving infants exposed to immunosuppressive agents during gestation."}
{"id": "PMID:484646", "title": "Obtaining drug exposure histories during pregnancy.", "content": "One hundred fifty-three pregnant patients were included in this study to verify the amount of drugs ingested during their pregnancies. The results demonstrate that, unknown to the physician, pregnant patients take a variety of pharmacologic agents. The patient's medical record is considered grossly inadequate in documenting an accurate assessment of a patient's exposure to drugs taken during pregnancy. Drug histories were obtained utilizing the hospital pharmacist drug history interview and home diary. Validity testing of the pharmacist's drug history and of the medical record history was not performed. The patient's compliance for utilizing the home diary for drugs or chemicals taken during their pregnancy was 83% (127 patients). It was shown that the usual methods of drug history documentation will identify only 30% of the actual drug exposure to the fetus.", "contents": "Obtaining drug exposure histories during pregnancy. One hundred fifty-three pregnant patients were included in this study to verify the amount of drugs ingested during their pregnancies. The results demonstrate that, unknown to the physician, pregnant patients take a variety of pharmacologic agents. The patient's medical record is considered grossly inadequate in documenting an accurate assessment of a patient's exposure to drugs taken during pregnancy. Drug histories were obtained utilizing the hospital pharmacist drug history interview and home diary. Validity testing of the pharmacist's drug history and of the medical record history was not performed. The patient's compliance for utilizing the home diary for drugs or chemicals taken during their pregnancy was 83% (127 patients). It was shown that the usual methods of drug history documentation will identify only 30% of the actual drug exposure to the fetus."}
{"id": "PMID:484647", "title": "Outcome of normal and dysfunctional labor in different racial groups.", "content": "Spontaneous labor in patients of different racial groups has been studied relating progress and outcome to whether labor was dysfunctional as defined by the partogram and action line. Forty-three percent of primigravidas and 17.6 to 25.8% of multigravidas passed the action line and had a lower admission cervical dilatation and a longer observed first stage than those patients whose labor progress remained to the left of the action line. White and black primigravidas whose labor progressed to the right of the action line had lower 1 and 5 minute Apgar scores and delivered heavier babies than those to the left. The cesarean section rates were 1.6% and 1.4% (left) and 7.6% and 18.2% (right) in white and black primigravidas, respectively. The cesarean section rate was significantly higher in black primigravidas irrespective of the relationship to the action line due to the high incidence of the complications of hypertension such as fetal distress and abruptio placentae in those in normal labor as well as those in dysfunctional labor due to cephalopelvic disproportion in those patients whose cervimetric progress went to the right of the action line.", "contents": "Outcome of normal and dysfunctional labor in different racial groups. Spontaneous labor in patients of different racial groups has been studied relating progress and outcome to whether labor was dysfunctional as defined by the partogram and action line. Forty-three percent of primigravidas and 17.6 to 25.8% of multigravidas passed the action line and had a lower admission cervical dilatation and a longer observed first stage than those patients whose labor progress remained to the left of the action line. White and black primigravidas whose labor progressed to the right of the action line had lower 1 and 5 minute Apgar scores and delivered heavier babies than those to the left. The cesarean section rates were 1.6% and 1.4% (left) and 7.6% and 18.2% (right) in white and black primigravidas, respectively. The cesarean section rate was significantly higher in black primigravidas irrespective of the relationship to the action line due to the high incidence of the complications of hypertension such as fetal distress and abruptio placentae in those in normal labor as well as those in dysfunctional labor due to cephalopelvic disproportion in those patients whose cervimetric progress went to the right of the action line."}
{"id": "PMID:484648", "title": "In vitro effect of dopamine and pimozide on human chorionic gonadotropin secretion.", "content": "In human placental explants cultured in vitro, dopamine inhibited human chorionic gonadotropin (hCG) secretion into the culture media. In the control flasks, the level of hCG secretion was 751 +/- 215 mIU/gm of tissue. When 1 mM of dopamine was added, hCG levels decreased to 321 +/- 57.6 mIU/gm of tissue (n = 6, P less than 0.1)--5 and 10 mM of dopamine significantly inhibited hCG secretion. In contrast, 1 mM of pimozide enhanced hCG secretion by 1.8-fold compared to control levels (1,707 +/- 343 versus 3,117 +/- 0.005). This in vitro effect on hCG is similar to the effect of dopamine and pimozide on hCS secretion by placental explants.", "contents": "In vitro effect of dopamine and pimozide on human chorionic gonadotropin secretion. In human placental explants cultured in vitro, dopamine inhibited human chorionic gonadotropin (hCG) secretion into the culture media. In the control flasks, the level of hCG secretion was 751 +/- 215 mIU/gm of tissue. When 1 mM of dopamine was added, hCG levels decreased to 321 +/- 57.6 mIU/gm of tissue (n = 6, P less than 0.1)--5 and 10 mM of dopamine significantly inhibited hCG secretion. In contrast, 1 mM of pimozide enhanced hCG secretion by 1.8-fold compared to control levels (1,707 +/- 343 versus 3,117 +/- 0.005). This in vitro effect on hCG is similar to the effect of dopamine and pimozide on hCS secretion by placental explants."}
{"id": "PMID:484649", "title": "Changes in the glycosaminoglycans distribution pattern in the human uterine cervix during pregnancy and labor.", "content": "The glycosaminoglycans distribution pattern of uterine cervix samples obtained from 42 women of reproductive age was determined by means of proteolytic digestion and subsequent chromatographic separation. The following glycosaminoglycans were detected: chondroitin 4- and 6-sulfates, dermatan sulfate, hyaluronate, chondroitin, and keratan sulfate. The connective tissue of the uterine cervix shows a characteristic distribution pattern with regard to glycosaminoglycans which does not correspond to that found in any other tissue studied so far. Based on dry weight, the content of keratan sulfate increases during pregnancy while the concentration of chondroitin remains unchanged. The chondroitin sulfates and dermatan sulfate drop simultaneously. During labor chondroitin increases threefold. The hyaluronate content of the postpartum cervix is higher than that of the cervix in nonpregnant women. Both changes in the solubility of collagen as well as in the distribution pattern of the glycosaminoglycans seem to be related to cervical dilatation.", "contents": "Changes in the glycosaminoglycans distribution pattern in the human uterine cervix during pregnancy and labor. The glycosaminoglycans distribution pattern of uterine cervix samples obtained from 42 women of reproductive age was determined by means of proteolytic digestion and subsequent chromatographic separation. The following glycosaminoglycans were detected: chondroitin 4- and 6-sulfates, dermatan sulfate, hyaluronate, chondroitin, and keratan sulfate. The connective tissue of the uterine cervix shows a characteristic distribution pattern with regard to glycosaminoglycans which does not correspond to that found in any other tissue studied so far. Based on dry weight, the content of keratan sulfate increases during pregnancy while the concentration of chondroitin remains unchanged. The chondroitin sulfates and dermatan sulfate drop simultaneously. During labor chondroitin increases threefold. The hyaluronate content of the postpartum cervix is higher than that of the cervix in nonpregnant women. Both changes in the solubility of collagen as well as in the distribution pattern of the glycosaminoglycans seem to be related to cervical dilatation."}
{"id": "PMID:484650", "title": "Fetal breathing movements and the nonstress test in high-risk pregnancies.", "content": "Three hundred ninety-eight observations of fetal breathing movements (FBM) and the nonstress test (NST) were made in 223 patients. The presence of FBM was noted in a significantly higher proportion of tests with a reactive NST (88%) than a nonreactive NST (67%). Conversely, a significantly higher proportion of tests were reactive when FBM were present (82.6%) than when FBM were absent (49%). A significant relationship existed betweeen either test and the outcome of pregnancy as judged by the five minute Apgar score or the incidence of fetal distress in labor. Combining two normal tests did not improve the accuracy in predicting outcome; but the combination of both tests, when abnormal, produced a significant improvement in predicting fetuses likely to have an abnormal outcome. The combination of the normal with the abnormal test had a predictive accuracy similar to a single normal test in predicting a normal five-minute Apgar. This combination was associated with an incidence of fetal distress in labor intermediate between that seen with either the normal or abnormal test when alone. Neither a single test nor the combination of tests was helpful in identifying the small-for-gestational age (SGA) infants. These data indicate antepartum fetal evaluation may be improved when more than one biophysical variable is used.", "contents": "Fetal breathing movements and the nonstress test in high-risk pregnancies. Three hundred ninety-eight observations of fetal breathing movements (FBM) and the nonstress test (NST) were made in 223 patients. The presence of FBM was noted in a significantly higher proportion of tests with a reactive NST (88%) than a nonreactive NST (67%). Conversely, a significantly higher proportion of tests were reactive when FBM were present (82.6%) than when FBM were absent (49%). A significant relationship existed betweeen either test and the outcome of pregnancy as judged by the five minute Apgar score or the incidence of fetal distress in labor. Combining two normal tests did not improve the accuracy in predicting outcome; but the combination of both tests, when abnormal, produced a significant improvement in predicting fetuses likely to have an abnormal outcome. The combination of the normal with the abnormal test had a predictive accuracy similar to a single normal test in predicting a normal five-minute Apgar. This combination was associated with an incidence of fetal distress in labor intermediate between that seen with either the normal or abnormal test when alone. Neither a single test nor the combination of tests was helpful in identifying the small-for-gestational age (SGA) infants. These data indicate antepartum fetal evaluation may be improved when more than one biophysical variable is used."}
{"id": "PMID:484651", "title": "The relative significance of human placental lactogen in the diagnosis of retarded fetal growth.", "content": "The purpose of this prospective study was to determine whether serial maternal venous hPL determinations could identify pregnancies resulting in growth-retarded infants from a selected population at presumed high risk for IUGR. Our results demonstrated that mean hPL levels in IUGR outcome pregnancies were significantly lower than normal after 33 weeks' gestation. Mean hPL was also lower in some pregnancies resulting in normal-weight neonates with abnormally low PI or short CHL, suggesting that these neonates, despite normal birth weight achievement, may represent previously unsuspected.", "contents": "The relative significance of human placental lactogen in the diagnosis of retarded fetal growth. The purpose of this prospective study was to determine whether serial maternal venous hPL determinations could identify pregnancies resulting in growth-retarded infants from a selected population at presumed high risk for IUGR. Our results demonstrated that mean hPL levels in IUGR outcome pregnancies were significantly lower than normal after 33 weeks' gestation. Mean hPL was also lower in some pregnancies resulting in normal-weight neonates with abnormally low PI or short CHL, suggesting that these neonates, despite normal birth weight achievement, may represent previously unsuspected."}
{"id": "PMID:484652", "title": "Insulin and placenta: degradation and stabilization, binding to microvillous membrane receptors, and amino acid uptake.", "content": "Human placenta possesses insulin receptors, yet whether insulin affects uptake of substrate or other placental functions is still controversial. A potential problem in the study of this issue is degradation of insulin by placental tissue. We found insulin degradation to be rapid and temperature dependent. At 10 mU/ml and 37 degrees C, 40% became trichloroacetic acid soluble within 15 minutes. Degradation was decreased by preincubation of tissue, followed by change of medium and by addition of 0.5% to 2.0% bovine albumin. Receptor assay with isolated microvillous membrane, which bound insulin with the characteristics of known target tissues, demonstrated biologically active insulin (5 to 6 mU/ml) to persist under incubation conditions. The presence of insulin did not influence the uptake of amino acids by the three neutral amino acid transport systems of the human placenta. Thus the transport of an essential fetal nutrient is is apparently independent of fluctuations in maternal insulin with dietary carbohydrate intake and disease.", "contents": "Insulin and placenta: degradation and stabilization, binding to microvillous membrane receptors, and amino acid uptake. Human placenta possesses insulin receptors, yet whether insulin affects uptake of substrate or other placental functions is still controversial. A potential problem in the study of this issue is degradation of insulin by placental tissue. We found insulin degradation to be rapid and temperature dependent. At 10 mU/ml and 37 degrees C, 40% became trichloroacetic acid soluble within 15 minutes. Degradation was decreased by preincubation of tissue, followed by change of medium and by addition of 0.5% to 2.0% bovine albumin. Receptor assay with isolated microvillous membrane, which bound insulin with the characteristics of known target tissues, demonstrated biologically active insulin (5 to 6 mU/ml) to persist under incubation conditions. The presence of insulin did not influence the uptake of amino acids by the three neutral amino acid transport systems of the human placenta. Thus the transport of an essential fetal nutrient is is apparently independent of fluctuations in maternal insulin with dietary carbohydrate intake and disease."}
{"id": "PMID:484653", "title": "Simultaneous determination of seven unconjugated steroids in maternal venous and umbilical arterial and venous serum in elective and emergency cesarean section at term.", "content": "In order to assess specific gluco- and mineralocorticoid functions in both mother and fetoplacental unit in relation to the presence or absence of labor, serum levels of unconjugated aldosterone (A), corticosterone (B), deoxycorticosterone (DOC), progesterone (P), 17-hydroxyprogesterone (17-OHP), cortisol (F), and cortisone (E) were determined simultaneously. These levels were determined by specific radioimmunoassays in two groups of 24 paired maternal venous and umbilical arterial and venous samples obtained at term delivery by either elective (Group I, N = 8) or emergency (Group II, N = 8) cesarean section. In Group II, after spontaneous labor, mean maternal serum levels of all steroids investigated exceeded those found in Group I (not in labor). These increases were most pronounced (p less than 0.005) in F (74%) and DOC (106%) levels demonstrating stimulation of both the glucocorticoid (cortisol)--and the mineralocorticoid (aldosterone)--producing pathways of the maternal adrenals by spontaneous labor. Arteriovenous differences in umbilical steroid levels revealed in both groups the placental origin of P, 17-OHP, and E (p less than 0.05 to 0.005), with greater (more negative) mean AV differences after labor (Group II). The negative AV difference of DOC, B, A, and F found in Group I, however, decreased after labor and became even positive in the cases of B and F, reflecting the close relationship between spontaneous labor and the fetal adrenal's active production not only of the glucocorticoids B and F, but also, to a lesser extent, of the mineralocorticoids DOC and aldosterone.", "contents": "Simultaneous determination of seven unconjugated steroids in maternal venous and umbilical arterial and venous serum in elective and emergency cesarean section at term. In order to assess specific gluco- and mineralocorticoid functions in both mother and fetoplacental unit in relation to the presence or absence of labor, serum levels of unconjugated aldosterone (A), corticosterone (B), deoxycorticosterone (DOC), progesterone (P), 17-hydroxyprogesterone (17-OHP), cortisol (F), and cortisone (E) were determined simultaneously. These levels were determined by specific radioimmunoassays in two groups of 24 paired maternal venous and umbilical arterial and venous samples obtained at term delivery by either elective (Group I, N = 8) or emergency (Group II, N = 8) cesarean section. In Group II, after spontaneous labor, mean maternal serum levels of all steroids investigated exceeded those found in Group I (not in labor). These increases were most pronounced (p less than 0.005) in F (74%) and DOC (106%) levels demonstrating stimulation of both the glucocorticoid (cortisol)--and the mineralocorticoid (aldosterone)--producing pathways of the maternal adrenals by spontaneous labor. Arteriovenous differences in umbilical steroid levels revealed in both groups the placental origin of P, 17-OHP, and E (p less than 0.05 to 0.005), with greater (more negative) mean AV differences after labor (Group II). The negative AV difference of DOC, B, A, and F found in Group I, however, decreased after labor and became even positive in the cases of B and F, reflecting the close relationship between spontaneous labor and the fetal adrenal's active production not only of the glucocorticoids B and F, but also, to a lesser extent, of the mineralocorticoids DOC and aldosterone."}
{"id": "PMID:484661", "title": "Ocular myasthenia gravis mimicking pseudointernuclear ophthalmoplegia and variable esotropia.", "content": "A 70-year-old man with known vascular disease, who had bilateral internuclear ophthalmoplegia, and an 18-month-old girl with a long-term variable esotropia culminating in a postinfectious oculopharyngeal neuropathy both responded with a positive edrophonium chloride (Tensilon) test, strongly suggesting the diagnosis of myasthenia gravis. Both subsequently received maintenance doses of neostigmine methylsulfate (Prostigmin).", "contents": "Ocular myasthenia gravis mimicking pseudointernuclear ophthalmoplegia and variable esotropia. A 70-year-old man with known vascular disease, who had bilateral internuclear ophthalmoplegia, and an 18-month-old girl with a long-term variable esotropia culminating in a postinfectious oculopharyngeal neuropathy both responded with a positive edrophonium chloride (Tensilon) test, strongly suggesting the diagnosis of myasthenia gravis. Both subsequently received maintenance doses of neostigmine methylsulfate (Prostigmin)."}
{"id": "PMID:484662", "title": "Vitreous fluorophotometry in adult-onset diabetes mellitus.", "content": "We studied the breakdown of the blood-retinal barrier in 83 patients with adult-onset diabetes mellitus, by using vitreous fluorophotometry. The patients were in good stable metabolic control and were receiving injectible insulin or oral hypoglycemic agents. Vitreous fluorophotometry readings were abnormally high but similar in both groups of patients, and no significant differences were found between those with and those without early background diabetic retinopathy. Significantly more patients taking oral agents were hypertensive, and significantly fewer of these patients had background retinopathy.", "contents": "Vitreous fluorophotometry in adult-onset diabetes mellitus. We studied the breakdown of the blood-retinal barrier in 83 patients with adult-onset diabetes mellitus, by using vitreous fluorophotometry. The patients were in good stable metabolic control and were receiving injectible insulin or oral hypoglycemic agents. Vitreous fluorophotometry readings were abnormally high but similar in both groups of patients, and no significant differences were found between those with and those without early background diabetic retinopathy. Significantly more patients taking oral agents were hypertensive, and significantly fewer of these patients had background retinopathy."}
{"id": "PMID:484663", "title": "Localized conjunctival amyloidosis.", "content": "We treated a patient with localized amyloidosis of the bulbar conjunctiva, which strongly resembled a neoplastic growth. Although the patient suffered from a long-standing, debilitating rheumatoid arthritis, suggesting the possibility of a secondary systemic amyloidosis caused by the chronic effusive process, no specific clinical evidence of systemic amyloidosis was noted. The ocular lesion was, therefore, classified as a primary localized amyloidosis. However, the simple recognition of amyloid in the ocular adnexa should not be considered a complete diagnosis. For such cases in which a prominent underlying systemic disease is present, a thorough medical evaluation and future follow-up should be ensured to rule out the possibility that this represents the initial manifestation of a potentially life-threatening secondary systemic amyloidosis caused by rheumatoid arthritis.", "contents": "Localized conjunctival amyloidosis. We treated a patient with localized amyloidosis of the bulbar conjunctiva, which strongly resembled a neoplastic growth. Although the patient suffered from a long-standing, debilitating rheumatoid arthritis, suggesting the possibility of a secondary systemic amyloidosis caused by the chronic effusive process, no specific clinical evidence of systemic amyloidosis was noted. The ocular lesion was, therefore, classified as a primary localized amyloidosis. However, the simple recognition of amyloid in the ocular adnexa should not be considered a complete diagnosis. For such cases in which a prominent underlying systemic disease is present, a thorough medical evaluation and future follow-up should be ensured to rule out the possibility that this represents the initial manifestation of a potentially life-threatening secondary systemic amyloidosis caused by rheumatoid arthritis."}
{"id": "PMID:484664", "title": "Extended-wear contact lenses in 525 aphakic eyes.", "content": "Extended-wear contact lenses were fitted to 225 patients with monocular aphakia and 150 with bilateral aphakia. They were observed from three months to three years. Eighty-eight of the 195 eyes fitted with a Softcon lens became red and painful within 14 days during extended wear. The Hydrocurve lens was better tolerated in 55, but many patients had acute congestion, pain, and tearing. Deposits accumulated rapidly on the Sauflon-PW lens. Some 90% of all patients can be fitted successfully with the cellulose acetate butyrate lens or the silicone lens. The silicone coating of the silicone lens is lost or damaged in about 20% of the patients because of excess mucous accumulation.", "contents": "Extended-wear contact lenses in 525 aphakic eyes. Extended-wear contact lenses were fitted to 225 patients with monocular aphakia and 150 with bilateral aphakia. They were observed from three months to three years. Eighty-eight of the 195 eyes fitted with a Softcon lens became red and painful within 14 days during extended wear. The Hydrocurve lens was better tolerated in 55, but many patients had acute congestion, pain, and tearing. Deposits accumulated rapidly on the Sauflon-PW lens. Some 90% of all patients can be fitted successfully with the cellulose acetate butyrate lens or the silicone lens. The silicone coating of the silicone lens is lost or damaged in about 20% of the patients because of excess mucous accumulation."}
{"id": "PMID:484665", "title": "Epithelial deposition of gold in the cornea in patients receiving systemic therapy.", "content": "Epithelial gold deposits were demonstrated biomicroscopically in 13 of 15 patients receiving gold therapy for rheumatoid arthritis. Only one of six further patients who had received gold therapy in the past showed such deposits. Deposits appeared with a cumulative dose of sodium thiomalate exceeding 100 mg. The earliest appearance was after seven months of therapy and deposits were visible in one subject as long as nine months after therapy had stopped. The density of deposit does not appear to increase over the whole of the dose range, possibly as a result of the effect of epithelial turnover. No symptoms attributable to the deposit were encountered in any patient.", "contents": "Epithelial deposition of gold in the cornea in patients receiving systemic therapy. Epithelial gold deposits were demonstrated biomicroscopically in 13 of 15 patients receiving gold therapy for rheumatoid arthritis. Only one of six further patients who had received gold therapy in the past showed such deposits. Deposits appeared with a cumulative dose of sodium thiomalate exceeding 100 mg. The earliest appearance was after seven months of therapy and deposits were visible in one subject as long as nine months after therapy had stopped. The density of deposit does not appear to increase over the whole of the dose range, possibly as a result of the effect of epithelial turnover. No symptoms attributable to the deposit were encountered in any patient."}
{"id": "PMID:484666", "title": "In vivo choroidal thickness measurement.", "content": "We developed a system that permits exceptionally accurate measurements of human choroidal thickness in vivo by means of ultrasound. With minicomputer techniques, the complementary functions of the time and spectral domains of reflected sound can be used to permit measurements accurate to less than 20 mu at 10 MHz center frequency. Even more accuracy can be obtained with higher center frequencies. Choroidal thickness has been found to exceed that previously reported, with typical values of 420 mu found at the posterior pole. Even greater values are seen in regions outside the macula.", "contents": "In vivo choroidal thickness measurement. We developed a system that permits exceptionally accurate measurements of human choroidal thickness in vivo by means of ultrasound. With minicomputer techniques, the complementary functions of the time and spectral domains of reflected sound can be used to permit measurements accurate to less than 20 mu at 10 MHz center frequency. Even more accuracy can be obtained with higher center frequencies. Choroidal thickness has been found to exceed that previously reported, with typical values of 420 mu found at the posterior pole. Even greater values are seen in regions outside the macula."}
{"id": "PMID:484667", "title": "Effects of monomeric methylmethacrylate on ocular tissues.", "content": "Toxic effects of high doses of monomeric methylmethacrylate were demonstrated in rabbit eyes. These effects were not related to surgical manipulation. Monomeric methylmethacrylate caused limbal hyperemia, corneal edema, corneal neovascularization, iris engorgement, anterior chamber inflammation, iris atrophy, and cataract. The doses of monomeric methylmethacrylate needed to produce these lesions were much higher than the amount of monomer available for leaching out of implanted intraocular lenses.", "contents": "Effects of monomeric methylmethacrylate on ocular tissues. Toxic effects of high doses of monomeric methylmethacrylate were demonstrated in rabbit eyes. These effects were not related to surgical manipulation. Monomeric methylmethacrylate caused limbal hyperemia, corneal edema, corneal neovascularization, iris engorgement, anterior chamber inflammation, iris atrophy, and cataract. The doses of monomeric methylmethacrylate needed to produce these lesions were much higher than the amount of monomer available for leaching out of implanted intraocular lenses."}
{"id": "PMID:484668", "title": "Prospective observation of vascular anastomoses between the retina and choroid in recurrent toxoplasmosis.", "content": "Prospective angiographic observations demonstrated the formation of both choroidoretinal arteriolar anastomoses as well as retinochoroidal venular anastomoses in the ocular fundus of a young man with recurrent ocular toxoplasmosis.", "contents": "Prospective observation of vascular anastomoses between the retina and choroid in recurrent toxoplasmosis. Prospective angiographic observations demonstrated the formation of both choroidoretinal arteriolar anastomoses as well as retinochoroidal venular anastomoses in the ocular fundus of a young man with recurrent ocular toxoplasmosis."}
{"id": "PMID:484669", "title": "Prevention and treatment of symblepharon.", "content": "Symblepharon after acute conjunctival injury can be prevented by separating  the raw surfaces for one to two weeks, until re-epithelialization takes place. We have used a soft contact lens and a conjunctival ring without a corneal center, or lined the eyelid and palpebral conjunctiva with plastic. This often eliminates the need for mucous membrane grafts. Symblepharon can interfere with intraocular surgery and disrupt cataract incisions unless they are divided and separated at the time of surgery.", "contents": "Prevention and treatment of symblepharon. Symblepharon after acute conjunctival injury can be prevented by separating  the raw surfaces for one to two weeks, until re-epithelialization takes place. We have used a soft contact lens and a conjunctival ring without a corneal center, or lined the eyelid and palpebral conjunctiva with plastic. This often eliminates the need for mucous membrane grafts. Symblepharon can interfere with intraocular surgery and disrupt cataract incisions unless they are divided and separated at the time of surgery."}
{"id": "PMID:484670", "title": "Observations on the development of the anterior chamber angle with reference to the pathogenesis of congenital glaucomas.", "content": "Abnormalities in neural crest cell migration may have a role in the pathogenesis of the congenital glaucomas, with or without changes in the gonioscopic appearance of the anterior chamber angle. Neural crest cells also contribute to the bones of the face, dental papilla, cartilage, bone, and meninges; this may explain the association of craniofacial, dental, and upper spinal malformations with some of the congenital glaucomas.", "contents": "Observations on the development of the anterior chamber angle with reference to the pathogenesis of congenital glaucomas. Abnormalities in neural crest cell migration may have a role in the pathogenesis of the congenital glaucomas, with or without changes in the gonioscopic appearance of the anterior chamber angle. Neural crest cells also contribute to the bones of the face, dental papilla, cartilage, bone, and meninges; this may explain the association of craniofacial, dental, and upper spinal malformations with some of the congenital glaucomas."}
{"id": "PMID:484671", "title": "Ocular hypotensive action of labetalol.", "content": "Labetalol has a unique and profound effect on the IOP of rabbits, in contrast to pure beta-adrenergic blockers which have little or no effect in this animal. Its action cannot be satisfactorily explained by simple alpha and beta-adrenergic blocking activity. In the concentrations used, it failed to block the action of a beta-adrenergic agonist (isoproterenol), the action of an alpha-adrenegic agonist (norepinephrine), and its own hypotensive action was neither blocked nor potentiated by the alpha-adrenergic blocker phenoxybenzamine. However, the beta blocker, timolol, did reduce the action of labetalol. These observations and the reduced response to labetalol after cervical sympathectomy, suggest that labetalol's ocular hypotensive effect may occur through some mechanism other than alpha or beta blockade.", "contents": "Ocular hypotensive action of labetalol. Labetalol has a unique and profound effect on the IOP of rabbits, in contrast to pure beta-adrenergic blockers which have little or no effect in this animal. Its action cannot be satisfactorily explained by simple alpha and beta-adrenergic blocking activity. In the concentrations used, it failed to block the action of a beta-adrenergic agonist (isoproterenol), the action of an alpha-adrenegic agonist (norepinephrine), and its own hypotensive action was neither blocked nor potentiated by the alpha-adrenergic blocker phenoxybenzamine. However, the beta blocker, timolol, did reduce the action of labetalol. These observations and the reduced response to labetalol after cervical sympathectomy, suggest that labetalol's ocular hypotensive effect may occur through some mechanism other than alpha or beta blockade."}
{"id": "PMID:484672", "title": "Birth order and parental age in microphthalmos and other ocular diseases.", "content": "We compared the distribution of birth order and maternal and paternal ages of blind school children throughout Japan with that of the total Japanese population of the corresponding age groups and with that of a subgroup of children with acquired blindness. The number of first-born children with microphthalmos was smaller, and the number of second-, third-, or fourth-born children was larger, as compared with the control groups. The differences were highly statistically significant by chi-square test. There was a less pronounced indication of birth order effect in amblyopia, congenital cataract, and optic nerve atrophy, which involved more first-borns than in the controls. The distribution of maternal age was also different from the control group in microphthalmos, congenital cataract, corneal opacity, and optic nerve atrophy. Less mothers in their 20s and more in their 30s produced children with these conditions. We believe this finding may be partly related to the rapid decline in infant mortality and in the incidence of congenital blindness in Japan.", "contents": "Birth order and parental age in microphthalmos and other ocular diseases. We compared the distribution of birth order and maternal and paternal ages of blind school children throughout Japan with that of the total Japanese population of the corresponding age groups and with that of a subgroup of children with acquired blindness. The number of first-born children with microphthalmos was smaller, and the number of second-, third-, or fourth-born children was larger, as compared with the control groups. The differences were highly statistically significant by chi-square test. There was a less pronounced indication of birth order effect in amblyopia, congenital cataract, and optic nerve atrophy, which involved more first-borns than in the controls. The distribution of maternal age was also different from the control group in microphthalmos, congenital cataract, corneal opacity, and optic nerve atrophy. Less mothers in their 20s and more in their 30s produced children with these conditions. We believe this finding may be partly related to the rapid decline in infant mortality and in the incidence of congenital blindness in Japan."}
{"id": "PMID:484673", "title": "Vessels of intraocular malignant melanomas.", "content": "The vessel walls of six malignant choroidal melanomas were thin; only thin basal membranes and narrow endothelial linings could be observed with the electron microscope. The endothelium was even absent in some places. Tumor cells were released into the bloodstream through disruptions of the vessel walls in the epithelioid and spindle cell B type tumors.", "contents": "Vessels of intraocular malignant melanomas. The vessel walls of six malignant choroidal melanomas were thin; only thin basal membranes and narrow endothelial linings could be observed with the electron microscope. The endothelium was even absent in some places. Tumor cells were released into the bloodstream through disruptions of the vessel walls in the epithelioid and spindle cell B type tumors."}
{"id": "PMID:484674", "title": "Association of HLA antigens and primary open-angle glaucoma.", "content": "HLA testing of 55 patients with primary open-angle glaucoma revealed no increased frequencies of any of 17 HLA-A and 29 HLA-B antigens. However, the subgroup with a known family history of primary open-angle glaucoma had an increase in the frequency of the HLA-B12 antigen (48%). Intrafamily studies might further define the strength and importance of this association.", "contents": "Association of HLA antigens and primary open-angle glaucoma. HLA testing of 55 patients with primary open-angle glaucoma revealed no increased frequencies of any of 17 HLA-A and 29 HLA-B antigens. However, the subgroup with a known family history of primary open-angle glaucoma had an increase in the frequency of the HLA-B12 antigen (48%). Intrafamily studies might further define the strength and importance of this association."}
{"id": "PMID:484675", "title": "Pars plana vitrectomy in ocular trauma.", "content": "Visual improvement was achieved in 62% of 100 consecutive patients with ocular trauma treated by pars plana vitrectomy. Anterior segment injuries had a better prognosis than posterior segment injuries, and retinal detachment was a poor prognostic sign. Patients undergoing vitrectomy during the two weeks after injury had a better visual prognosis than those who had delayed vitrectomy. Pars plana vitrectomy has increased the recovery rate in traumatized eyes which previously were deemed inoperable and frequently were enucleated. Most such eyes have intraocular fibrocellular proliferations, resulting in traction retinal detachments, cyclitic membranes, and phthisis, as documented in clinicopathological and experimentally produced specimens of penetrating ocular trauma. Vitrectomy can interrupt this sequence, if performed one to 14 days after injury, by removing the vitreous scaffold onto which proliferation occurs, together with the elements of hemorrhage, damaged lens, vitreous, and foreign material which may incite proliferation. We believe four to ten days after injury to be the optimal time for vitrectomy to avoid the hazards of immediate intervention, while removing damaged tissue before serious sequenlae occur.", "contents": "Pars plana vitrectomy in ocular trauma. Visual improvement was achieved in 62% of 100 consecutive patients with ocular trauma treated by pars plana vitrectomy. Anterior segment injuries had a better prognosis than posterior segment injuries, and retinal detachment was a poor prognostic sign. Patients undergoing vitrectomy during the two weeks after injury had a better visual prognosis than those who had delayed vitrectomy. Pars plana vitrectomy has increased the recovery rate in traumatized eyes which previously were deemed inoperable and frequently were enucleated. Most such eyes have intraocular fibrocellular proliferations, resulting in traction retinal detachments, cyclitic membranes, and phthisis, as documented in clinicopathological and experimentally produced specimens of penetrating ocular trauma. Vitrectomy can interrupt this sequence, if performed one to 14 days after injury, by removing the vitreous scaffold onto which proliferation occurs, together with the elements of hemorrhage, damaged lens, vitreous, and foreign material which may incite proliferation. We believe four to ten days after injury to be the optimal time for vitrectomy to avoid the hazards of immediate intervention, while removing damaged tissue before serious sequenlae occur."}
{"id": "PMID:484676", "title": "Cataracts and macular degeneration.", "content": "The intraocular lens restores general vision and some degree of independence and mobility to patients with dense cataracts and macular degeneration. The patient, however, must be repeatedly warned that fine central vision, particularly reading, will not be possible after the surgery. An aphakic spectacle leaves such patients a narrow band of vision when superimposed over the macular lesion, and contact lenses are too small for the patient to manage insertion without help.", "contents": "Cataracts and macular degeneration. The intraocular lens restores general vision and some degree of independence and mobility to patients with dense cataracts and macular degeneration. The patient, however, must be repeatedly warned that fine central vision, particularly reading, will not be possible after the surgery. An aphakic spectacle leaves such patients a narrow band of vision when superimposed over the macular lesion, and contact lenses are too small for the patient to manage insertion without help."}
{"id": "PMID:484677", "title": "Estimates of genetic correlations among clinical measures of the eye.", "content": "In a quantitative genetic investigation of seven clinical tests, five pairs were significantly correlated: (1) cover test measures and corneal power, (2) corneal power and divergence recovery points, (3) convergence and divergence recovery points, (4) divergence break and recovery points, and (5) convergence break and recovery points. Common genes may account entirely for the gene influence on cover test measure; thus, parents who are above the population mean for corneal power will have offspring with a tendency toward an exodeviation (phoria). The two convergence amplitudes may depend on the same gene differences, whereas the two divergence amplitudes only partly reflect a common function, suggesting that they may be indexing somewhat different underlying physiologic mechanisms.", "contents": "Estimates of genetic correlations among clinical measures of the eye. In a quantitative genetic investigation of seven clinical tests, five pairs were significantly correlated: (1) cover test measures and corneal power, (2) corneal power and divergence recovery points, (3) convergence and divergence recovery points, (4) divergence break and recovery points, and (5) convergence break and recovery points. Common genes may account entirely for the gene influence on cover test measure; thus, parents who are above the population mean for corneal power will have offspring with a tendency toward an exodeviation (phoria). The two convergence amplitudes may depend on the same gene differences, whereas the two divergence amplitudes only partly reflect a common function, suggesting that they may be indexing somewhat different underlying physiologic mechanisms."}
{"id": "PMID:484678", "title": "Myelinated retinal nerve fibers associated with ipsilateral myopia, amblyopia, and strabismus.", "content": "Four patients had extensive unilateral myelinated nerve fibers associated with ipsilateral myopia, amblyopia, and strabismus. Their profound visual impairment, exotropia, and the early age onset of symptoms indicated that the amblyopia may have been organically caused. Prognosis is poor for even partial correction but good results have been obtained with intense therapy that includes full correction of the refractive error in each eye and extraocular muscle surgery if cosmetically necessary. In patients with axial myopia, images of a similar size will be produced by placing a lens of the correct power at the spectacle plane. In younger patients, a contact lens should not be used to correct the refractive error because it creates an undesirable anisometropia. Younger patients who have parafoveal fixation and no strabismus respond best to amblyopia therapy.", "contents": "Myelinated retinal nerve fibers associated with ipsilateral myopia, amblyopia, and strabismus. Four patients had extensive unilateral myelinated nerve fibers associated with ipsilateral myopia, amblyopia, and strabismus. Their profound visual impairment, exotropia, and the early age onset of symptoms indicated that the amblyopia may have been organically caused. Prognosis is poor for even partial correction but good results have been obtained with intense therapy that includes full correction of the refractive error in each eye and extraocular muscle surgery if cosmetically necessary. In patients with axial myopia, images of a similar size will be produced by placing a lens of the correct power at the spectacle plane. In younger patients, a contact lens should not be used to correct the refractive error because it creates an undesirable anisometropia. Younger patients who have parafoveal fixation and no strabismus respond best to amblyopia therapy."}
{"id": "PMID:484679", "title": "Penalization in the treatment of amblyopia.", "content": "Penalization is a useful alternative to occlusion therapy in amblyopia of mild and moderate degrees and in preserving therapeutic gains made by previous occlusion treatment. Penalization at near, total, and alternating penalization are more valuable than penalization at distance. Because penalization does not prevent the development of visual deprivation amblyopia, unilateral atropine therapy must be used carefully in treating patients in an age group susceptible to occlusion amblyopia.", "contents": "Penalization in the treatment of amblyopia. Penalization is a useful alternative to occlusion therapy in amblyopia of mild and moderate degrees and in preserving therapeutic gains made by previous occlusion treatment. Penalization at near, total, and alternating penalization are more valuable than penalization at distance. Because penalization does not prevent the development of visual deprivation amblyopia, unilateral atropine therapy must be used carefully in treating patients in an age group susceptible to occlusion amblyopia."}
{"id": "PMID:484680", "title": "An en bloc technique for the resection of anterior uveal tumors.", "content": "We modified trabeculo-iridocyclosclerectomy in a technique for en bloc excision of anterior uveal tumor. A trabeculectomy-type of approach was used, which allowed excision of a rectangular block of sclera, cornea, and trabeculum with attached uveal tissue containing the tumor.", "contents": "An en bloc technique for the resection of anterior uveal tumors. We modified trabeculo-iridocyclosclerectomy in a technique for en bloc excision of anterior uveal tumor. A trabeculectomy-type of approach was used, which allowed excision of a rectangular block of sclera, cornea, and trabeculum with attached uveal tissue containing the tumor."}
{"id": "PMID:484681", "title": "Metastatic disease from untreated uveal melanomas.", "content": "Few ophthalmologists or pathologists have observed uveal melanomas that metastasized before they were recognized and treated. In an effort to characterize those melanomas that have produced metastatic disease before the tumor-containing eye was enucleated, we have collected a series of 29 cases for review. These have generally involved older subjects (median age 65 years) who had large tumors that had been symptomatic for long periods before being recognized. A disproportionately large percentage showed extraocular extension. Although the data support the thesis that uveal melanomas are typically slow-growing tumors that show local infiltrative properties but little tendency to produce metastatic disease unless treated by enucleation of the eye, it is possible that the available information is possibly biased by our methods of acquisition of data. Much pertinent information is lost because the cases usually do not come to the attention of ophthalmologists or ophthalmic pathologist. This missing link in our knowledge of the natural course of untreated uveal melanomas is one of several factors that make it impossible to determine whether the overall effects of enucleation have been beneficial or harmful in the management of this disease.", "contents": "Metastatic disease from untreated uveal melanomas. Few ophthalmologists or pathologists have observed uveal melanomas that metastasized before they were recognized and treated. In an effort to characterize those melanomas that have produced metastatic disease before the tumor-containing eye was enucleated, we have collected a series of 29 cases for review. These have generally involved older subjects (median age 65 years) who had large tumors that had been symptomatic for long periods before being recognized. A disproportionately large percentage showed extraocular extension. Although the data support the thesis that uveal melanomas are typically slow-growing tumors that show local infiltrative properties but little tendency to produce metastatic disease unless treated by enucleation of the eye, it is possible that the available information is possibly biased by our methods of acquisition of data. Much pertinent information is lost because the cases usually do not come to the attention of ophthalmologists or ophthalmic pathologist. This missing link in our knowledge of the natural course of untreated uveal melanomas is one of several factors that make it impossible to determine whether the overall effects of enucleation have been beneficial or harmful in the management of this disease."}
{"id": "PMID:484682", "title": "Extended-wear contact lenses and intraocular lenses for aphakic correction.", "content": "We evaluated extended-wear soft contact lens use in 128 aphakic eyes of 113 patients, 44 of whom had been unable to wear conventional contact lenses; 116 eyes (91%) have been successfully fitted and have worn the extended-wear lenses without significant complication. Eleven patients were unable to wear the lens or had to be discontinued for transient lens-related problems. In 103 eyes successfully fitted the lens is removed for cleaning less often than monthly. Ninety six of the 116 eyes fitted have visual acuity as good as, or better than, with aphakic correcting spectacles. The results obtained with the extended-wear soft contact lens are similar to those obtained with intraocular lenses, except for the recent high incidence of inflammation seen after intraocular lens insertion when using ethylene-oxide-sterilized lenses.", "contents": "Extended-wear contact lenses and intraocular lenses for aphakic correction. We evaluated extended-wear soft contact lens use in 128 aphakic eyes of 113 patients, 44 of whom had been unable to wear conventional contact lenses; 116 eyes (91%) have been successfully fitted and have worn the extended-wear lenses without significant complication. Eleven patients were unable to wear the lens or had to be discontinued for transient lens-related problems. In 103 eyes successfully fitted the lens is removed for cleaning less often than monthly. Ninety six of the 116 eyes fitted have visual acuity as good as, or better than, with aphakic correcting spectacles. The results obtained with the extended-wear soft contact lens are similar to those obtained with intraocular lenses, except for the recent high incidence of inflammation seen after intraocular lens insertion when using ethylene-oxide-sterilized lenses."}
{"id": "PMID:484683", "title": "The microbial flora in extended-wear soft contact-lens wearers.", "content": "Of 857 cultures of material collected from the eyelids, conjunctivae, and contact lenses of 51 extended-wear soft-contact-lens wearers, 44 (5%) grew potential pathogens. The aphakic patients wearing contact lenses had fewer recoverable pathogens (24 of 295 or 8%) than a group of preoperative cataract patients (63 of 295 or 21%). On five occasions, positive cultures accompanied clinical symptoms, and on three occasions, repeated cultures grew the same potential pathogen.", "contents": "The microbial flora in extended-wear soft contact-lens wearers. Of 857 cultures of material collected from the eyelids, conjunctivae, and contact lenses of 51 extended-wear soft-contact-lens wearers, 44 (5%) grew potential pathogens. The aphakic patients wearing contact lenses had fewer recoverable pathogens (24 of 295 or 8%) than a group of preoperative cataract patients (63 of 295 or 21%). On five occasions, positive cultures accompanied clinical symptoms, and on three occasions, repeated cultures grew the same potential pathogen."}
{"id": "PMID:484684", "title": "The optical quality of currently manufactured intraocular lenses.", "content": "We selected 95 intraocular lenses from ten lens manufacturers for a study of optical quality. Tremendous variation in resolution from a high of 313 line pairs per millimeter to a low of 40 line pairs per millimeter was seen. All of the lenses from eight manufacturers showed greater than 100 line pairs per millimeter resolution, the American National Standards Institute proposed minimum. Five of the 95 lenses had greater than 1.0 diopter error, whereas 79 of the 95 lenses had less than 0.5 diopter error from the stated power. With respect to toricity, there were 11 lenses with greater than 0.25 diopter (built-in astigmatism), however, no lenses showed more than 0.5 diopter astigmatism. Two lenses had a built-in double image, another clinically significant problem. We believe the optical quality of the lenses from some lens manufacturers cannot be guaranteed.", "contents": "The optical quality of currently manufactured intraocular lenses. We selected 95 intraocular lenses from ten lens manufacturers for a study of optical quality. Tremendous variation in resolution from a high of 313 line pairs per millimeter to a low of 40 line pairs per millimeter was seen. All of the lenses from eight manufacturers showed greater than 100 line pairs per millimeter resolution, the American National Standards Institute proposed minimum. Five of the 95 lenses had greater than 1.0 diopter error, whereas 79 of the 95 lenses had less than 0.5 diopter error from the stated power. With respect to toricity, there were 11 lenses with greater than 0.25 diopter (built-in astigmatism), however, no lenses showed more than 0.5 diopter astigmatism. Two lenses had a built-in double image, another clinically significant problem. We believe the optical quality of the lenses from some lens manufacturers cannot be guaranteed."}
{"id": "PMID:484685", "title": "A glass intraocular lens.", "content": "We designed a glass and polyimide iris-plane, iris-clip intraocular lens. It is sterilized in the autoclave and weighs approximately 6 mg in water. The optic is 0.3 mm thick for a 19-diopter lens and the flexible haptic is 0.05 mm thick. Two identical haptics and the optic are joined mechanically to form the intraocular lens. The round spatula-like portion of an insertional instrument secures the intraocular lens by passing through two opposing flexible haptic loops and behind the optic. Fluid or air is irrigated through the cannula as the implant is inserted in order to protect the endothelium.", "contents": "A glass intraocular lens. We designed a glass and polyimide iris-plane, iris-clip intraocular lens. It is sterilized in the autoclave and weighs approximately 6 mg in water. The optic is 0.3 mm thick for a 19-diopter lens and the flexible haptic is 0.05 mm thick. Two identical haptics and the optic are joined mechanically to form the intraocular lens. The round spatula-like portion of an insertional instrument secures the intraocular lens by passing through two opposing flexible haptic loops and behind the optic. Fluid or air is irrigated through the cannula as the implant is inserted in order to protect the endothelium."}
{"id": "PMID:484686", "title": "Sodium hydroxide sterilzation of intraocular lenses.", "content": "Intraocular lenses of polymethylmethacrylate contaminated with various inocula of bacteria, spores, and fungi were sterilized by modifications of the Ridley method using sodium hydroxide. Positive cultures were obtained from iris-supported lenses contaminated with concentrations of approximately 1 x 10(6) organisms per milliliter after one hour in 10% NaOH. No positive cultures were obtained when lenses remained in 10% NaOH for three hours, a time corresponding to a 1 x 10(-6) probability of surviving organism. The NaOH method, when appropriately used, is an effective sterilization process for intraocular lenses.", "contents": "Sodium hydroxide sterilzation of intraocular lenses. Intraocular lenses of polymethylmethacrylate contaminated with various inocula of bacteria, spores, and fungi were sterilized by modifications of the Ridley method using sodium hydroxide. Positive cultures were obtained from iris-supported lenses contaminated with concentrations of approximately 1 x 10(6) organisms per milliliter after one hour in 10% NaOH. No positive cultures were obtained when lenses remained in 10% NaOH for three hours, a time corresponding to a 1 x 10(-6) probability of surviving organism. The NaOH method, when appropriately used, is an effective sterilization process for intraocular lenses."}
{"id": "PMID:484687", "title": "Evaluation of timolol maleate combination therapy in chronic open-angle glaucoma.", "content": "In an eight-week study, timolol maleate ophthalmic solution 0.25% was administered twice daily with either pilocarpine 2% four times a day, epinephrine 2% twice a day, carbachol 1.5% four times a day, or 250 mg of acetazolamide twice a day to 44 patients with chronic open-angle glaucoma. Compared to baseline readings on standard therapy alone, mean intraocular pressure was significantly reduced after addition of timolol maleate to each of the regimens. Mean facility of outflow was unaffected. No clinically significant ocular irritation or changes in visual acuity, pupil size, or vital signs were observed in any patient. Timolol maleate appears to be effective and well tolerated when administered with other antiglaucoma medications in patients with open-angle glaucoma.", "contents": "Evaluation of timolol maleate combination therapy in chronic open-angle glaucoma. In an eight-week study, timolol maleate ophthalmic solution 0.25% was administered twice daily with either pilocarpine 2% four times a day, epinephrine 2% twice a day, carbachol 1.5% four times a day, or 250 mg of acetazolamide twice a day to 44 patients with chronic open-angle glaucoma. Compared to baseline readings on standard therapy alone, mean intraocular pressure was significantly reduced after addition of timolol maleate to each of the regimens. Mean facility of outflow was unaffected. No clinically significant ocular irritation or changes in visual acuity, pupil size, or vital signs were observed in any patient. Timolol maleate appears to be effective and well tolerated when administered with other antiglaucoma medications in patients with open-angle glaucoma."}
{"id": "PMID:484688", "title": "Transient reversible cupping in juvenile-onset glaucoma.", "content": "A 17-year-old man had marked glaucomatous cupping of his left optic nerve. Two days after trabeculectomy, the appearance of the disk changed dramatically, appearing full, rather than cupped. Over the next month, with normal intraocular pressure, the optic nerve resumed its preoperative excavated appearance.", "contents": "Transient reversible cupping in juvenile-onset glaucoma. A 17-year-old man had marked glaucomatous cupping of his left optic nerve. Two days after trabeculectomy, the appearance of the disk changed dramatically, appearing full, rather than cupped. Over the next month, with normal intraocular pressure, the optic nerve resumed its preoperative excavated appearance."}
{"id": "PMID:484689", "title": "Circumferential flow in Schlemm's canal.", "content": "A model of Schlemm's canal was designed and analyzed mathematically. The effect of canal width on intraocular pressure was examined, and the pressure and flow distribution in the canal were calculated.", "contents": "Circumferential flow in Schlemm's canal. A model of Schlemm's canal was designed and analyzed mathematically. The effect of canal width on intraocular pressure was examined, and the pressure and flow distribution in the canal were calculated."}
{"id": "PMID:484690", "title": "Trabecular and retrocorneal proliferation of melanocytes and secondary glaucoma.", "content": "A 59-year-old woman with no evidence of glaucoma developed secondary open-angle glaucoma after an intracapsular cataract extraction complicated by vitreous and iris incarceration. Histopathologic and scanning and electron microscopic studies of the enucleated eye 22 years later revealed extensive proliferation of iris melanocytes across the trabecular meshwork and posterior surface of the cornea. Proliferation of iris stromal melanocytes over the trabecular meshwork may have caused secondary open-angle glaucoma.", "contents": "Trabecular and retrocorneal proliferation of melanocytes and secondary glaucoma. A 59-year-old woman with no evidence of glaucoma developed secondary open-angle glaucoma after an intracapsular cataract extraction complicated by vitreous and iris incarceration. Histopathologic and scanning and electron microscopic studies of the enucleated eye 22 years later revealed extensive proliferation of iris melanocytes across the trabecular meshwork and posterior surface of the cornea. Proliferation of iris stromal melanocytes over the trabecular meshwork may have caused secondary open-angle glaucoma."}
{"id": "PMID:484691", "title": "Blepharoptosis after traumatic third-nerve palsies.", "content": "We evaluated the clinical records of 16 patients with blepharoptosis secondary to direct traumatic third-nerve palsies. Most patients were involved in auto accidents, and had associated skull fractures. Of 12 patients who were followed up for at least one year, ten recovered completely. Over half of the patients had evidence of aberrant regeneration, which could be seen clinically as early as nine weeks after the trauma. Orbital localization of the third-nerve dysfunction carries an excellent prognosis for prompt, spontaneous recovery without aberrant regeneration. Surgical intervention in these cases should be delayed at least one year after the traumatic event, and longer if continued slow but progressive recovery is demonstrated.", "contents": "Blepharoptosis after traumatic third-nerve palsies. We evaluated the clinical records of 16 patients with blepharoptosis secondary to direct traumatic third-nerve palsies. Most patients were involved in auto accidents, and had associated skull fractures. Of 12 patients who were followed up for at least one year, ten recovered completely. Over half of the patients had evidence of aberrant regeneration, which could be seen clinically as early as nine weeks after the trauma. Orbital localization of the third-nerve dysfunction carries an excellent prognosis for prompt, spontaneous recovery without aberrant regeneration. Surgical intervention in these cases should be delayed at least one year after the traumatic event, and longer if continued slow but progressive recovery is demonstrated."}
{"id": "PMID:484692", "title": "Effect of isolated inferior oblique muscle myectomy in the management of superior oblique muscle palsy.", "content": "Thirty-three patients with superior oblique palsy were treated with an inferior oblique myectomy temporal to the inferior rectus muscle. The mean effect of surgery was 11.91 +/- 1.38 in all positions of gaze and for all age groups. The operative result in both children and adults was the same. The mean effect of surgery correlated with the size of the preoperative hyperdeviation in the fields of action of both inferior and superior oblique muscles. Inferior oblique muscle myectomy was followed by return of normal function to the superior oblique muscle in 63% of cases. Ocular torticollis was eliminated in 90% of the patients. Persistent overaction of inferior oblique muscle was encountered in three patients. Postoperative underaction of the myectomized muscle was not observed in any of our patients. A myectomy is recommended as a primary procedure in patients with superior oblique muscle palsy when the direct antagonist is overacting and the hyperdeviation is 10 to 15 prism diopters or more in primary position or the fields of action of the paretic superior oblique muscle or the overacting inferior oblique muscle.", "contents": "Effect of isolated inferior oblique muscle myectomy in the management of superior oblique muscle palsy. Thirty-three patients with superior oblique palsy were treated with an inferior oblique myectomy temporal to the inferior rectus muscle. The mean effect of surgery was 11.91 +/- 1.38 in all positions of gaze and for all age groups. The operative result in both children and adults was the same. The mean effect of surgery correlated with the size of the preoperative hyperdeviation in the fields of action of both inferior and superior oblique muscles. Inferior oblique muscle myectomy was followed by return of normal function to the superior oblique muscle in 63% of cases. Ocular torticollis was eliminated in 90% of the patients. Persistent overaction of inferior oblique muscle was encountered in three patients. Postoperative underaction of the myectomized muscle was not observed in any of our patients. A myectomy is recommended as a primary procedure in patients with superior oblique muscle palsy when the direct antagonist is overacting and the hyperdeviation is 10 to 15 prism diopters or more in primary position or the fields of action of the paretic superior oblique muscle or the overacting inferior oblique muscle."}
{"id": "PMID:484693", "title": "Mydriatic effect of anticholinergic drugs used during reversal of nondepolarizing muscle relaxants.", "content": "Large doses of anticholinergic drugs (atropine, glycopyrrolate) produced mydriasis in a group of adults with no eye abnormalities except strabismus, though the usual intramuscular and intravenous doses of these drugs do not have this tendency. Such large doses are often given intravenously during general anesthesia to prevent the side effects of neostigmine methylsulfate, which is used to reverse the effect of nondepolarizing muscle relaxants. Neostigmine methylsulfate (Prostigmin) reduced the mydriatic effect when given intravenously in conjunction with atropine or glycopyrrolate. Mydriasis was more likely to occur in lightly pigmented eyes than in eyes with dark irides. Pilocarpine eyedrops instilled at the beginning of anesthesia caused miosis that persisted after the large intravenous doses of atropine or glycopyrrolate were given. To prevent an attack of acute angle-closure glaucoma in any patient who is to receive large doses of anticholinergic drugs during general anesthesia, miotic drug therapy should be continued before, during, and after anesthesia at the same frequency as when awake.", "contents": "Mydriatic effect of anticholinergic drugs used during reversal of nondepolarizing muscle relaxants. Large doses of anticholinergic drugs (atropine, glycopyrrolate) produced mydriasis in a group of adults with no eye abnormalities except strabismus, though the usual intramuscular and intravenous doses of these drugs do not have this tendency. Such large doses are often given intravenously during general anesthesia to prevent the side effects of neostigmine methylsulfate, which is used to reverse the effect of nondepolarizing muscle relaxants. Neostigmine methylsulfate (Prostigmin) reduced the mydriatic effect when given intravenously in conjunction with atropine or glycopyrrolate. Mydriasis was more likely to occur in lightly pigmented eyes than in eyes with dark irides. Pilocarpine eyedrops instilled at the beginning of anesthesia caused miosis that persisted after the large intravenous doses of atropine or glycopyrrolate were given. To prevent an attack of acute angle-closure glaucoma in any patient who is to receive large doses of anticholinergic drugs during general anesthesia, miotic drug therapy should be continued before, during, and after anesthesia at the same frequency as when awake."}
{"id": "PMID:484694", "title": "Pseudo-orbital apex syndrome.", "content": "A 48-year-old man had symptoms and signs of the orbital apex syndrome. All orbital and systemic studies were unrevealing . The computed tomoggraphy scan showed a cystic lesion external to the pons. The lesion proved to be a cholesteotoma originating in the peduncular fossa area, comprising the oculomotor nerve proximally and extending anteriorly in a finger-like projection to involve the ipsilateral optic nerve.", "contents": "Pseudo-orbital apex syndrome. A 48-year-old man had symptoms and signs of the orbital apex syndrome. All orbital and systemic studies were unrevealing . The computed tomoggraphy scan showed a cystic lesion external to the pons. The lesion proved to be a cholesteotoma originating in the peduncular fossa area, comprising the oculomotor nerve proximally and extending anteriorly in a finger-like projection to involve the ipsilateral optic nerve."}
{"id": "PMID:484697", "title": "The measurement of the Bausch & Lomb Soflens contact lens by interferometry.", "content": "A method is described for the measurement of the anterior and posterior radii of curvature and thickness or a soft (hydrogel) contact lens. The method is based on the observation of an interference pattern that is generated by two beams of light, one reflected from the surface of the lens and the other reflected from the surface of a comparison mirror. It was found that the maximum coefficient of variation for the measured values of the anterior and posterior radii of curvature was 2% and the minimum value was 0.6%. An accuracy of 1:10(3) for the radii of curvature and the center thickness of the lens is claimed.", "contents": "The measurement of the Bausch & Lomb Soflens contact lens by interferometry. A method is described for the measurement of the anterior and posterior radii of curvature and thickness or a soft (hydrogel) contact lens. The method is based on the observation of an interference pattern that is generated by two beams of light, one reflected from the surface of the lens and the other reflected from the surface of a comparison mirror. It was found that the maximum coefficient of variation for the measured values of the anterior and posterior radii of curvature was 2% and the minimum value was 0.6%. An accuracy of 1:10(3) for the radii of curvature and the center thickness of the lens is claimed."}
{"id": "PMID:484698", "title": "Prism-ballasted and truncated spherical trial lenses as indicators of toric soft lens rotation.", "content": "Fifteen refractive astigmats were fitted with prism ballasted and truncated plano, spherical lenses and prescription toric soft lenses. The initial rotation observed with each lens type did not differ significantly, indicating that trial sets of such spherical lenses may provide an effective method of fitting toric soft lenses.", "contents": "Prism-ballasted and truncated spherical trial lenses as indicators of toric soft lens rotation. Fifteen refractive astigmats were fitted with prism ballasted and truncated plano, spherical lenses and prescription toric soft lenses. The initial rotation observed with each lens type did not differ significantly, indicating that trial sets of such spherical lenses may provide an effective method of fitting toric soft lenses."}
{"id": "PMID:484699", "title": "The relationship between convergence insufficiency and school achievement.", "content": "Schoolchildren (735) between the ages of 7 and 14 were examined for convergence ability. Nine percent had an objective near point of convergence of more than 10 cm and 10% had a subjective near point of convergence of more than 10 cm. The recovery point was greater than 15 cm for 9% of the subjects when measured objectively and 12% subjectively. The sample was analyzed with regard to age, sex, and IQ. There was no significant difference between children who showed convergence insufficiency and those who did not with regard to school results in their mother tongue and in their grade point average.", "contents": "The relationship between convergence insufficiency and school achievement. Schoolchildren (735) between the ages of 7 and 14 were examined for convergence ability. Nine percent had an objective near point of convergence of more than 10 cm and 10% had a subjective near point of convergence of more than 10 cm. The recovery point was greater than 15 cm for 9% of the subjects when measured objectively and 12% subjectively. The sample was analyzed with regard to age, sex, and IQ. There was no significant difference between children who showed convergence insufficiency and those who did not with regard to school results in their mother tongue and in their grade point average."}
{"id": "PMID:484700", "title": "Factors affecting peripheral curve design.", "content": "Factors associated with edge clearance in the contact lens-corneal relationship are peripheral curve radius and width, lens diameter, and corneal shape. The effect of each parameter is quantified, permitting an examination of current design practices. Recommendations are that minor adjustments be made by changing radius, that major adjustments be made by changing width, and that the peripheral curve radius should be steepened as the diameter increases.", "contents": "Factors affecting peripheral curve design. Factors associated with edge clearance in the contact lens-corneal relationship are peripheral curve radius and width, lens diameter, and corneal shape. The effect of each parameter is quantified, permitting an examination of current design practices. Recommendations are that minor adjustments be made by changing radius, that major adjustments be made by changing width, and that the peripheral curve radius should be steepened as the diameter increases."}
{"id": "PMID:484701", "title": "Convergence and divergence show large and sustained improvement after short isometric exercise.", "content": "There are conflicting reports on the effect of vergence training. In two studies using push up and prism vergence exercises the conflict is shown to result from differences in the constancy of effort. Improvement only occurs in exercises involving sustained effort in the direction being trained. The effect of alternating movements in both directions is small and inconsistent. Substantial longlasting gains in either convergence or divergence prism vergence scores can result from just 5 min of sustained effort at an angle halfway between the break and recovery points. Results for adults and children on motor-driven instruments are comparable and the scores obtained discriminate better than those from hand-turned instruments. The most likely physiological model involves potentiating processes which are specifically maximized by isometric exercise. Potentiation results in a positive feedback process, the effects of which are strong enough to precipitate strabismus and which might be deliberately manipulated in therapy of poor vergence control, if responses in the required direction can be induced.", "contents": "Convergence and divergence show large and sustained improvement after short isometric exercise. There are conflicting reports on the effect of vergence training. In two studies using push up and prism vergence exercises the conflict is shown to result from differences in the constancy of effort. Improvement only occurs in exercises involving sustained effort in the direction being trained. The effect of alternating movements in both directions is small and inconsistent. Substantial longlasting gains in either convergence or divergence prism vergence scores can result from just 5 min of sustained effort at an angle halfway between the break and recovery points. Results for adults and children on motor-driven instruments are comparable and the scores obtained discriminate better than those from hand-turned instruments. The most likely physiological model involves potentiating processes which are specifically maximized by isometric exercise. Potentiation results in a positive feedback process, the effects of which are strong enough to precipitate strabismus and which might be deliberately manipulated in therapy of poor vergence control, if responses in the required direction can be induced."}
{"id": "PMID:484702", "title": "Near retinoscopy and cycloplegic retinoscopy in early primary grade schoolchildren.", "content": "Retinoscopy under cycloplegia with various agents can yield varied results. Additionally, cycloplegics and produce adverse local as well as central nervous system disturbances. A noncycloplegic near retinoscopy technique as promulgated by Mohindra is compared with cycloplegic retinoscopy on schoolchildren in early grades. Results obtained by a study utilizing the two techniques show that they yield similar objective measurements of refraction.", "contents": "Near retinoscopy and cycloplegic retinoscopy in early primary grade schoolchildren. Retinoscopy under cycloplegia with various agents can yield varied results. Additionally, cycloplegics and produce adverse local as well as central nervous system disturbances. A noncycloplegic near retinoscopy technique as promulgated by Mohindra is compared with cycloplegic retinoscopy on schoolchildren in early grades. Results obtained by a study utilizing the two techniques show that they yield similar objective measurements of refraction."}
{"id": "PMID:484703", "title": "Strength of thin chemtempered lenses: static load testing.", "content": "Static load tests were conducted on heat-tempered and chemtempered plano white crown glass lenses from five different optical laboratories. With both ball-on-ring and ring-on-ring loading, chemtempered lenses considerably thinner than 2.0 mm were found to be as failure resistant as 2.0-mm-thick heat-tempered lenses. A similar result was obtained previously using the drop-ball test. It is shown that the theory of brittle fracture can be used to relate the results of different tests and provides a rational basis for comparing the relative performance of chemtempered and heat-tempered lenses.", "contents": "Strength of thin chemtempered lenses: static load testing. Static load tests were conducted on heat-tempered and chemtempered plano white crown glass lenses from five different optical laboratories. With both ball-on-ring and ring-on-ring loading, chemtempered lenses considerably thinner than 2.0 mm were found to be as failure resistant as 2.0-mm-thick heat-tempered lenses. A similar result was obtained previously using the drop-ball test. It is shown that the theory of brittle fracture can be used to relate the results of different tests and provides a rational basis for comparing the relative performance of chemtempered and heat-tempered lenses."}
{"id": "PMID:484705", "title": "A versatile multi-input device to study the Pulfrich effect.", "content": "A versatile multi-input device based on an x-y recorder was designed for the study of the Pulfrich effect. Because the device provided constant velocity of the target, a rectangular Pulfrich effect was observed rather than the traditional elliptical effect. The instrument permits study of the response induced when the input is changed in a variety of ways.", "contents": "A versatile multi-input device to study the Pulfrich effect. A versatile multi-input device based on an x-y recorder was designed for the study of the Pulfrich effect. Because the device provided constant velocity of the target, a rectangular Pulfrich effect was observed rather than the traditional elliptical effect. The instrument permits study of the response induced when the input is changed in a variety of ways."}
{"id": "PMID:484706", "title": "Calibration of the ERG photostimulus with standard photometer and photodiode.", "content": "A method for quantifying the ERG photostimulus is presented. The units of measure are reasonably straightforward and yet consistent with laws of photochemistry and psychophysics. Application of these laws facilitates measurement with readily available photometric instrumentation.", "contents": "Calibration of the ERG photostimulus with standard photometer and photodiode. A method for quantifying the ERG photostimulus is presented. The units of measure are reasonably straightforward and yet consistent with laws of photochemistry and psychophysics. Application of these laws facilitates measurement with readily available photometric instrumentation."}
{"id": "PMID:484707", "title": "Central gyrate atrophy: a case report.", "content": "Central gyrate atrophy is a rare ocular finding with a unique pathogenesis and fundus appearance. This entity results in total atrophy of the large and small vessels of the choroid and concomitant changes in the outer layers of the retina. A case report is presented to demonstrate the clinical appearance of this lesion and its relationship to the pathogenesis.", "contents": "Central gyrate atrophy: a case report. Central gyrate atrophy is a rare ocular finding with a unique pathogenesis and fundus appearance. This entity results in total atrophy of the large and small vessels of the choroid and concomitant changes in the outer layers of the retina. A case report is presented to demonstrate the clinical appearance of this lesion and its relationship to the pathogenesis."}
{"id": "PMID:484708", "title": "Infrared recording retinoscope for monitoring accomodation.", "content": "An optometer (infrared recording retinoscope) that provides high resolution measurements of accommodative changes of the eye has been developed. The instrument employs the principle of retinoscopy to monitor changes in the refractive state of the eye. Infrared radiation is scanned across the eye to provide a moving retinal source as in retinoscopy. An electro-optical system replaces the observer in determining the direction and amount of apparent motion in the pupil of the patient. The optometer is sensitive to accommodative changes of 0.12 D over a range of 12 D. The response of the instrument is faster than the most rapid changes of accommodation, and measurements are unaffected by pupil changes or by small eye movements.", "contents": "Infrared recording retinoscope for monitoring accomodation. An optometer (infrared recording retinoscope) that provides high resolution measurements of accommodative changes of the eye has been developed. The instrument employs the principle of retinoscopy to monitor changes in the refractive state of the eye. Infrared radiation is scanned across the eye to provide a moving retinal source as in retinoscopy. An electro-optical system replaces the observer in determining the direction and amount of apparent motion in the pupil of the patient. The optometer is sensitive to accommodative changes of 0.12 D over a range of 12 D. The response of the instrument is faster than the most rapid changes of accommodation, and measurements are unaffected by pupil changes or by small eye movements."}
{"id": "PMID:484709", "title": "Fluorescein as an aid in pachometry.", "content": "Precision of measurement of apparent corneal thickness with the Haag-Streit lamp depth measuring attachment is significantly enhanced by staining the precorneal film with sodium fluorescein before measuring.", "contents": "Fluorescein as an aid in pachometry. Precision of measurement of apparent corneal thickness with the Haag-Streit lamp depth measuring attachment is significantly enhanced by staining the precorneal film with sodium fluorescein before measuring."}
{"id": "PMID:484710", "title": "An accessory fill-in flash for the Nikon photo-slit lamp.", "content": "High-quality slit lamp photographs require a balance between slit beam exposure and exposure of surrounding ocular structures. Proper exposure of surrounding ocular structure is difficult to obtain with the Nikon photo-slit lamp. The purpose of this research was to design an accessory fill-in flash for this slit lamp, which would provide proper exposure of over all ocular structures. In addition, this paper describes the procedures used to obtain proper exposure values and provides an exposure table for this accessory flash unit.", "contents": "An accessory fill-in flash for the Nikon photo-slit lamp. High-quality slit lamp photographs require a balance between slit beam exposure and exposure of surrounding ocular structures. Proper exposure of surrounding ocular structure is difficult to obtain with the Nikon photo-slit lamp. The purpose of this research was to design an accessory fill-in flash for this slit lamp, which would provide proper exposure of over all ocular structures. In addition, this paper describes the procedures used to obtain proper exposure values and provides an exposure table for this accessory flash unit."}
{"id": "PMID:484711", "title": "Malpractice insurance at optometric institutions.", "content": "The optometric educator needs insurance against malpractice suits. A survey was made of the policies available at twelve schools and colleges of optometry in the United States. The object of this survey was to compare the various protections offered by the optometric institutions and also to inform the educators of what is available to them. It was found that coverage and premiums vary considerably between the institutions. At only one of the twelve schools and colleges of optometry responding to this survey were faculty members required to pay for their own policies, but to avoid undue risk, two other colleges highly recommended that their faculty purchase their own coverage. Implications of the low number of claims brought against optometric teaching facilities and other factors are discussed in light of the \"malpractice insurance crisis.\"", "contents": "Malpractice insurance at optometric institutions. The optometric educator needs insurance against malpractice suits. A survey was made of the policies available at twelve schools and colleges of optometry in the United States. The object of this survey was to compare the various protections offered by the optometric institutions and also to inform the educators of what is available to them. It was found that coverage and premiums vary considerably between the institutions. At only one of the twelve schools and colleges of optometry responding to this survey were faculty members required to pay for their own policies, but to avoid undue risk, two other colleges highly recommended that their faculty purchase their own coverage. Implications of the low number of claims brought against optometric teaching facilities and other factors are discussed in light of the \"malpractice insurance crisis.\""}
{"id": "PMID:484712", "title": "The theory and computation of optical modifications to the cornea in refractive keratoplasty.", "content": "The optical theory of keratophakia employing implant lenticules with a refractive index different from that of the cornea is presented. This generalized theory also embraces keratophakia employing donated stromal lenticules and keratomileusis, with equal accuracy. The method of solution of the formulas is indicated. The effects of the various parameters upon the radius of curvature to be applied to the cornea are illustrated and discussed.", "contents": "The theory and computation of optical modifications to the cornea in refractive keratoplasty. The optical theory of keratophakia employing implant lenticules with a refractive index different from that of the cornea is presented. This generalized theory also embraces keratophakia employing donated stromal lenticules and keratomileusis, with equal accuracy. The method of solution of the formulas is indicated. The effects of the various parameters upon the radius of curvature to be applied to the cornea are illustrated and discussed."}
{"id": "PMID:484713", "title": "A comparison of three methods for determining the reading addition.", "content": "Three subjective determinations of the reading addition required by each of 10 subjects were obtained in this study with the use of a cross cylinder grid target, a variable color, near-point, grid target and an ordinary reading card. All three methods gave similar results. In addition, there was no change in the measurement when the color of the horizontal and vertical lines was reversed on the variable color, near-point, grid target.", "contents": "A comparison of three methods for determining the reading addition. Three subjective determinations of the reading addition required by each of 10 subjects were obtained in this study with the use of a cross cylinder grid target, a variable color, near-point, grid target and an ordinary reading card. All three methods gave similar results. In addition, there was no change in the measurement when the color of the horizontal and vertical lines was reversed on the variable color, near-point, grid target."}
{"id": "PMID:484714", "title": "Prevalence and management of divergence excess.", "content": "The prevalence of divergence excess was assessed from the records of 250 consecutive patients attending a binocular-vision clinic. The 67 cases in the sample showing divergence excess were subdivided into the following groups: (1) secondary to operations for esotropia (8 cases); (2) anisometropic amblyopes (14 cases); (3) basic (14 cases); (4) convergence weakness (13 cases); (5) divergence excess (18 cases). Treatment methods placed heavy emphasis on physiological diplopia. Of the 18 in the divergence excess category, 14 began treatment, 10 showed a satisfactory outcome, two achieved some improvement, and two discontinued treatment on their own. Divergence excess seems to respond well to nonsurgical treatment.", "contents": "Prevalence and management of divergence excess. The prevalence of divergence excess was assessed from the records of 250 consecutive patients attending a binocular-vision clinic. The 67 cases in the sample showing divergence excess were subdivided into the following groups: (1) secondary to operations for esotropia (8 cases); (2) anisometropic amblyopes (14 cases); (3) basic (14 cases); (4) convergence weakness (13 cases); (5) divergence excess (18 cases). Treatment methods placed heavy emphasis on physiological diplopia. Of the 18 in the divergence excess category, 14 began treatment, 10 showed a satisfactory outcome, two achieved some improvement, and two discontinued treatment on their own. Divergence excess seems to respond well to nonsurgical treatment."}
{"id": "PMID:484715", "title": "Prevalence of the Hudson-St\u00e4hli line of the cornea.", "content": "The prevalence of the corneal Hudson-St\u00e4hli line among 400 eyes was found to range from 5.3% of the eyes of patients aged 11-20 years to 100% of eyes of patients aged 81-90 years. In 87.3% of patients over 50 years of age corneas were affected; this percentage is considerably higher than previous estimates. Up to the age of 60 years it occurs more frequently in men than in women.", "contents": "Prevalence of the Hudson-St\u00e4hli line of the cornea. The prevalence of the corneal Hudson-St\u00e4hli line among 400 eyes was found to range from 5.3% of the eyes of patients aged 11-20 years to 100% of eyes of patients aged 81-90 years. In 87.3% of patients over 50 years of age corneas were affected; this percentage is considerably higher than previous estimates. Up to the age of 60 years it occurs more frequently in men than in women."}
{"id": "PMID:484716", "title": "The incidence of cone epiphyses and ivory epiphyses of the hand in Nigerian children.", "content": "Radiographs of the left hand of 594 children from Ibadan, Nigeria were inspected for skeletal anomalies to determine differences in the incidence of cone and ivory epiphyses for the rich and poor children and differences in incidence for four hemoglobin types. Ivory epiphyses ovvurred more often in children in the lower socio-economic class and children with hemoglobin AA. The cone epiphyses observed in Hb SS children were primarily a result of bone infarcts, while cones in children of other hemoglobin types appeared to be normal variants. The incidence of ivory epiphyses was higher in the Nigerian children than in American black children in the Ten-State Nutrition Survey. The incidence of cone epiphyses was much lower than those reported for other normal populations.", "contents": "The incidence of cone epiphyses and ivory epiphyses of the hand in Nigerian children. Radiographs of the left hand of 594 children from Ibadan, Nigeria were inspected for skeletal anomalies to determine differences in the incidence of cone and ivory epiphyses for the rich and poor children and differences in incidence for four hemoglobin types. Ivory epiphyses ovvurred more often in children in the lower socio-economic class and children with hemoglobin AA. The cone epiphyses observed in Hb SS children were primarily a result of bone infarcts, while cones in children of other hemoglobin types appeared to be normal variants. The incidence of ivory epiphyses was higher in the Nigerian children than in American black children in the Ten-State Nutrition Survey. The incidence of cone epiphyses was much lower than those reported for other normal populations."}
{"id": "PMID:484717", "title": "A multinational Andean genetic and health program. VIII. Lung function changes with migration between altitudes.", "content": "Studies of lung function in high altitude populations have suggested the influence of hypoxic environment on the development of this characteristic independent of confounding variables such as ethnicity and habitual exercise. However, often the effect of altitude on vital capacity is greater in children than adults, suggesting that more than developmental adaptation is operative. Also selective migration could account for the similarity of migrants and permanent residents at a destination altitude. To explore these problems we studied the lung function (FVC, FEV1, PFR) of 377 individuals who had migrated between altitudes in northern Chile. Migrant measurements were adjusted to those of permanent residents of appropriate age, sex and height at the altitudes of origin and destination. The measurements were then related to ethnicity (Spanish-Aymara ancestry), occupation and permanence, the latter combining information on both age at migration to and length of stay at a destination altitude. Upward migration was associated with increased chest depth, FVC and FEV1, but not height or other chest measurements. Downward migration had no significant effect. The flow-dependent test PFR was so sensitive to observer variability and occupation that it was difficult to establish its relationship to permanence. Unlike the body measurements, lung function measurements (especially PFR) tended to deviate from permanent controls at the origin altitude in a direction suggestive of selective migration, nor was permanence itself independent of ethnicity and occupation. Because of these difficulties the question of developmental adaptation in lung function may not be answerable in cross-sectional studies like the present and previous efforts, but rather in longitudinal investigations in which the control is the individual him/herself.", "contents": "A multinational Andean genetic and health program. VIII. Lung function changes with migration between altitudes. Studies of lung function in high altitude populations have suggested the influence of hypoxic environment on the development of this characteristic independent of confounding variables such as ethnicity and habitual exercise. However, often the effect of altitude on vital capacity is greater in children than adults, suggesting that more than developmental adaptation is operative. Also selective migration could account for the similarity of migrants and permanent residents at a destination altitude. To explore these problems we studied the lung function (FVC, FEV1, PFR) of 377 individuals who had migrated between altitudes in northern Chile. Migrant measurements were adjusted to those of permanent residents of appropriate age, sex and height at the altitudes of origin and destination. The measurements were then related to ethnicity (Spanish-Aymara ancestry), occupation and permanence, the latter combining information on both age at migration to and length of stay at a destination altitude. Upward migration was associated with increased chest depth, FVC and FEV1, but not height or other chest measurements. Downward migration had no significant effect. The flow-dependent test PFR was so sensitive to observer variability and occupation that it was difficult to establish its relationship to permanence. Unlike the body measurements, lung function measurements (especially PFR) tended to deviate from permanent controls at the origin altitude in a direction suggestive of selective migration, nor was permanence itself independent of ethnicity and occupation. Because of these difficulties the question of developmental adaptation in lung function may not be answerable in cross-sectional studies like the present and previous efforts, but rather in longitudinal investigations in which the control is the individual him/herself."}
{"id": "PMID:484718", "title": "Monthly changes in the gain and loss of growth in weight of children living in Guatemala.", "content": "Monthly increments of weight growth for a sample of 246 Guatemala City private school children are analyzed for the presence of a seasonal pattern in rates of growth. Neither a seasonal pattern nor any other periodic rhythm is found. It is observed that a significantly greater number of children aged 5.0 to 6.9 years experience their minimum annual growth rate during the dry season, with up to 60% of them losing or not gaining weight in any one month. Patterns of diet, exercise and disease cannot explain this trend. A possible association between minimum weight growth and maximum growth in height is discussed.", "contents": "Monthly changes in the gain and loss of growth in weight of children living in Guatemala. Monthly increments of weight growth for a sample of 246 Guatemala City private school children are analyzed for the presence of a seasonal pattern in rates of growth. Neither a seasonal pattern nor any other periodic rhythm is found. It is observed that a significantly greater number of children aged 5.0 to 6.9 years experience their minimum annual growth rate during the dry season, with up to 60% of them losing or not gaining weight in any one month. Patterns of diet, exercise and disease cannot explain this trend. A possible association between minimum weight growth and maximum growth in height is discussed."}
{"id": "PMID:484719", "title": "Some neurophysiologic aspects of individual behavior.", "content": "The author provides a brief primer of the biologic processes in the brain that produce affect and implement communication. These neuroanatomic and neurophysiologic processes are presented in broad configuration rather than in detail and as distributive systems rather than just as localized mechanisms. The basic viewpoint is that of a continuum between the biologic processes within the brain and their manifestation as behavioral phenomena expressed in, and visible to, the culture. The primary focus is not clinical but on neuroanatomy and neurophysiology as basic medical sciences for psychiatry.", "contents": "Some neurophysiologic aspects of individual behavior. The author provides a brief primer of the biologic processes in the brain that produce affect and implement communication. These neuroanatomic and neurophysiologic processes are presented in broad configuration rather than in detail and as distributive systems rather than just as localized mechanisms. The basic viewpoint is that of a continuum between the biologic processes within the brain and their manifestation as behavioral phenomena expressed in, and visible to, the culture. The primary focus is not clinical but on neuroanatomy and neurophysiology as basic medical sciences for psychiatry."}
{"id": "PMID:484720", "title": "Normal neuropsychological abilities of alcoholic men in their late thirties.", "content": "The neuropsychological performance of alcoholic men in their late 30s (mean age, 37 years), some of whom has been abstinent for 3 weeks and others for 18 months, was compared with that of a group of men who drank occasionally. There were no differences between the alcoholic groups and the comparison group that could not be attributed to normal aging. The number of years of alcoholism or estimated lifetime alcohol use, dietary adequacy, and medical or blackout history did not relate to neuropsychological impairment. The authors suggest that a simple \"dose-effect\" relationship between alcohol use and cognitive decline cannot be assumed.", "contents": "Normal neuropsychological abilities of alcoholic men in their late thirties. The neuropsychological performance of alcoholic men in their late 30s (mean age, 37 years), some of whom has been abstinent for 3 weeks and others for 18 months, was compared with that of a group of men who drank occasionally. There were no differences between the alcoholic groups and the comparison group that could not be attributed to normal aging. The number of years of alcoholism or estimated lifetime alcohol use, dietary adequacy, and medical or blackout history did not relate to neuropsychological impairment. The authors suggest that a simple \"dose-effect\" relationship between alcohol use and cognitive decline cannot be assumed."}
{"id": "PMID:484721", "title": "Midlife concerns of women: implications of the menopause.", "content": "Midlife has received recent attention but is still difficult to define. Women's developmental phases are most appropriately understood as different from men's, with a complex integration of biological context, family development and roles, and individual development. Menopause has been considered a determining event, and a variety of symptoms have been attributed to menopausal changes. Emerging data indicate that menopause does not appear to be responsible for most of the symptoms. Midlife stresses are the result of a combination of personal, family, social, and biological variables, with postmenopausal development an important phase.", "contents": "Midlife concerns of women: implications of the menopause. Midlife has received recent attention but is still difficult to define. Women's developmental phases are most appropriately understood as different from men's, with a complex integration of biological context, family development and roles, and individual development. Menopause has been considered a determining event, and a variety of symptoms have been attributed to menopausal changes. Emerging data indicate that menopause does not appear to be responsible for most of the symptoms. Midlife stresses are the result of a combination of personal, family, social, and biological variables, with postmenopausal development an important phase."}
{"id": "PMID:484722", "title": "Choline chloride treatment of memory deficits in the elderly.", "content": "Eight elderly patients with mild memory impairment were given choline chloride, a drug that increases brain acetylcholine concentrations. After 16 g/day of choline for 7 days, average memory performance was not different from performance during pre- and postcholine placebo treatment, although the patient with the poorest baseline performance improved considerably on choline.", "contents": "Choline chloride treatment of memory deficits in the elderly. Eight elderly patients with mild memory impairment were given choline chloride, a drug that increases brain acetylcholine concentrations. After 16 g/day of choline for 7 days, average memory performance was not different from performance during pre- and postcholine placebo treatment, although the patient with the poorest baseline performance improved considerably on choline."}
{"id": "PMID:484723", "title": "Adaptive strategies and recovery from rape.", "content": "The authors conducted a follow-up study of 81 rape victims to analyze the effect of adaptive or maladaptive response to rape on recovery over a 4-6 year period. They found that victims recovering fastest used more adaptive strategies, including positive self-assessment; defense mechanisms of explanation, minimization, suppression, and dramatization; and increased action. Victims who had not yet recovered had more maladaptive mechanisms, such as negative self-assessments, inaction, substance abuse, and action on suicidal thoughts.", "contents": "Adaptive strategies and recovery from rape. The authors conducted a follow-up study of 81 rape victims to analyze the effect of adaptive or maladaptive response to rape on recovery over a 4-6 year period. They found that victims recovering fastest used more adaptive strategies, including positive self-assessment; defense mechanisms of explanation, minimization, suppression, and dramatization; and increased action. Victims who had not yet recovered had more maladaptive mechanisms, such as negative self-assessments, inaction, substance abuse, and action on suicidal thoughts."}
{"id": "PMID:484724", "title": "Battered parents: a new syndrome.", "content": "The authors identify a new syndrome of family violence--parent battering. Relevant dynamics include individual characteristics of the parent batterer, distortions in the generational authority hierarchy, the role of secrets and denial, and cultural influences. The authors conclude, on the basis of their clinical work with these families, that this subtype of family violence is distinct from child and spouse abuse.", "contents": "Battered parents: a new syndrome. The authors identify a new syndrome of family violence--parent battering. Relevant dynamics include individual characteristics of the parent batterer, distortions in the generational authority hierarchy, the role of secrets and denial, and cultural influences. The authors conclude, on the basis of their clinical work with these families, that this subtype of family violence is distinct from child and spouse abuse."}
{"id": "PMID:484725", "title": "The psychiatrist as medical backup: ambiguity in the delegation of clinical responsibility.", "content": "When the psychiatrist functions as medical backup, i.e., in a setting of delegated clinical responsibility, a number of problems may arise, originating from factors in the patient, in the physician, in the staff member to whom responsibility is delegated, and in the setting itself. The authors delineate these problem areas through case examples and discuss the implications for multidisciplinary training.", "contents": "The psychiatrist as medical backup: ambiguity in the delegation of clinical responsibility. When the psychiatrist functions as medical backup, i.e., in a setting of delegated clinical responsibility, a number of problems may arise, originating from factors in the patient, in the physician, in the staff member to whom responsibility is delegated, and in the setting itself. The authors delineate these problem areas through case examples and discuss the implications for multidisciplinary training."}
{"id": "PMID:484726", "title": "Termination initiated by the therapist: a countertransference storm.", "content": "Termination of psychotherapy has been infrequently examined in the literature and often ignored by many training programs. Not only are criteria and techniques for termination not often discussed, but termination as a therapeutic opportunity has been neglected. The authors present two cases of psychotherapy termination initiated by the therapist and point out that countertransference phenomena, potentially destructive, are the major aspect separating psychotherapy termination by mutual agreement and termination initiated by the therapist. Careful, regular, and attentive supervision for therapists in training is essential to counterbalance the intensified countertransference reactions involved.", "contents": "Termination initiated by the therapist: a countertransference storm. Termination of psychotherapy has been infrequently examined in the literature and often ignored by many training programs. Not only are criteria and techniques for termination not often discussed, but termination as a therapeutic opportunity has been neglected. The authors present two cases of psychotherapy termination initiated by the therapist and point out that countertransference phenomena, potentially destructive, are the major aspect separating psychotherapy termination by mutual agreement and termination initiated by the therapist. Careful, regular, and attentive supervision for therapists in training is essential to counterbalance the intensified countertransference reactions involved."}
{"id": "PMID:484727", "title": "The incidence of seizures among children with autistic symptoms.", "content": "The authors examined 183 children with autistic symptoms and found that the age-specific incidence rates of seizures in this sample were between 3 and 28 times the rates for children in the general population. The subjects classified as totally autistic were at high risk of developing seizure from early childhood well into adolescence, but especially so at puberty. The partially autistic children had an increased risk of seizures only up to age 10. The authors suggest that the high incidence of seizures at puberty observed in this study may be specific to children with total autistic symptomatology and may represent a distinct pathological process associated with autism.", "contents": "The incidence of seizures among children with autistic symptoms. The authors examined 183 children with autistic symptoms and found that the age-specific incidence rates of seizures in this sample were between 3 and 28 times the rates for children in the general population. The subjects classified as totally autistic were at high risk of developing seizure from early childhood well into adolescence, but especially so at puberty. The partially autistic children had an increased risk of seizures only up to age 10. The authors suggest that the high incidence of seizures at puberty observed in this study may be specific to children with total autistic symptomatology and may represent a distinct pathological process associated with autism."}
{"id": "PMID:484728", "title": "Temporal disorganization and primary affective disorder.", "content": "The authors used a self-rating scale of thinking process disorganization to measure degree of temporal disorganization in a group of 38 rigorously categorized psychiatric inpatients. Patients diagnosed as having primary affective disorder, depressed type, were significantly different from those with either a character disorder or schizophrenic diagnosis; both schizophrenic and character disorder groups showed variable temporal disorganization scores, and the primary affective disorder group showed consistently high levels of temporal disorganization. Diagnosis was more important than symptom measures in relationship to temporal disorganization scores.", "contents": "Temporal disorganization and primary affective disorder. The authors used a self-rating scale of thinking process disorganization to measure degree of temporal disorganization in a group of 38 rigorously categorized psychiatric inpatients. Patients diagnosed as having primary affective disorder, depressed type, were significantly different from those with either a character disorder or schizophrenic diagnosis; both schizophrenic and character disorder groups showed variable temporal disorganization scores, and the primary affective disorder group showed consistently high levels of temporal disorganization. Diagnosis was more important than symptom measures in relationship to temporal disorganization scores."}
{"id": "PMID:484729", "title": "A comparison of two unilateral ECT electrode placements: efficacy and electrical energy considerations.", "content": "The authors compared the treatment outcomes and ECT energy requirements of 10 patients treated with the Muller unilateral placement method (secondary electrode in the frontal position) with those of 10 matched patients treated with the unilateral placement described by d'Elia (secondary electrode lateral to the vertex). Both groups showed a similar dramatic clinical improvement after ECT; however, the mean watt seconds required for the Muller method was nearly four times that for the d'Elia procedure. The treating physicians reported that the patients treated by the d'Elia method had fewer anterograde and retrograde memory disturbances and that they were alert and oriented sooner after ECT.", "contents": "A comparison of two unilateral ECT electrode placements: efficacy and electrical energy considerations. The authors compared the treatment outcomes and ECT energy requirements of 10 patients treated with the Muller unilateral placement method (secondary electrode in the frontal position) with those of 10 matched patients treated with the unilateral placement described by d'Elia (secondary electrode lateral to the vertex). Both groups showed a similar dramatic clinical improvement after ECT; however, the mean watt seconds required for the Muller method was nearly four times that for the d'Elia procedure. The treating physicians reported that the patients treated by the d'Elia method had fewer anterograde and retrograde memory disturbances and that they were alert and oriented sooner after ECT."}
{"id": "PMID:484730", "title": "Breaking the cycle in abusive families.", "content": "The tendency for the cycle of abusive and neglectful parenting to be transmitted across generations has been well documented. The authors report on 40 families who broke this family pattern. The mechanisms for change used in these families included reliance on a broad network of resources, a degree of self-differentiation, an attitude of realistic optimism, and the ability to marshall extra resources to meet crisis situations. The authors recommend further study of such exceptions and a more hopeful approach to the problem of abusive families.", "contents": "Breaking the cycle in abusive families. The tendency for the cycle of abusive and neglectful parenting to be transmitted across generations has been well documented. The authors report on 40 families who broke this family pattern. The mechanisms for change used in these families included reliance on a broad network of resources, a degree of self-differentiation, an attitude of realistic optimism, and the ability to marshall extra resources to meet crisis situations. The authors recommend further study of such exceptions and a more hopeful approach to the problem of abusive families."}
{"id": "PMID:484752", "title": "Footedness of left- and right-handers.", "content": "The performance on a simple tapping task of the hands and feet of left- and right-handers was tested. Right-handers tapped faster with their right hand and right foot. Left-handers tapped faster with their left hand and right foot. Thus, footedness follows handedness in right-handers but not in left-handers. Left-handers showed smaller left/right differences than right-handers in both hand and foot performance. These data are in loose agreement with the modified genetic theory of handedness proposed by Annett (Hand preference and the laterality of cerebral speech, Cortex, 1975).", "contents": "Footedness of left- and right-handers. The performance on a simple tapping task of the hands and feet of left- and right-handers was tested. Right-handers tapped faster with their right hand and right foot. Left-handers tapped faster with their left hand and right foot. Thus, footedness follows handedness in right-handers but not in left-handers. Left-handers showed smaller left/right differences than right-handers in both hand and foot performance. These data are in loose agreement with the modified genetic theory of handedness proposed by Annett (Hand preference and the laterality of cerebral speech, Cortex, 1975)."}
{"id": "PMID:484753", "title": "Characteristics of men most likely to respond to an invitation to be screened.", "content": "Some of the characteristics of men, with known risk factors, who were most likely to respond to an invitation to be screened in a national randomized clinical trial to prevent heart disease were determined in 18,872 men, 35-57 years of age, members of the Kaiser Foundation Health Plan of Oregon. Demographic characteristics and risk factor variables (blood pressure, blood cholesterol, and cigarette smoking levels) were abstracted from medical records. The men were ranked high priority or low priority according to level of risk. All age-eligible men in the health plan received at least one invitation to be screened, with high-priority men receiving more invitations. Despite concentrated efforts to bring them in, less than one-half the high-priority men were screened. Participants were older and wealthier than non-participants, and more likely to have more dependents and to routinely use medical services. Whether or not a man received a medical care service within the preceding two years was a powerful discriminating variable in both the univariate and multivariate analyses reported. The findings suggest that health care programs serving a stable population group should give more consideration to screening in routine medical care.", "contents": "Characteristics of men most likely to respond to an invitation to be screened. Some of the characteristics of men, with known risk factors, who were most likely to respond to an invitation to be screened in a national randomized clinical trial to prevent heart disease were determined in 18,872 men, 35-57 years of age, members of the Kaiser Foundation Health Plan of Oregon. Demographic characteristics and risk factor variables (blood pressure, blood cholesterol, and cigarette smoking levels) were abstracted from medical records. The men were ranked high priority or low priority according to level of risk. All age-eligible men in the health plan received at least one invitation to be screened, with high-priority men receiving more invitations. Despite concentrated efforts to bring them in, less than one-half the high-priority men were screened. Participants were older and wealthier than non-participants, and more likely to have more dependents and to routinely use medical services. Whether or not a man received a medical care service within the preceding two years was a powerful discriminating variable in both the univariate and multivariate analyses reported. The findings suggest that health care programs serving a stable population group should give more consideration to screening in routine medical care."}
{"id": "PMID:484754", "title": "Utilization of prepaid dental health care by students in health professional schools.", "content": "Utilization of prepaid dental health care was studied, involving health professional students in an open panel program at the University of Alabama Medical Center. Major barriers to treatment were eliminated and optimal conditions for utilization established. Annual utilization ranged from 44-61 per cent during the period extending from 1969-70 to 1975-76. The majority of enrollees treated sought care from the Student Dental Health Plan (SDHP) clinic, rather than from private practitioners. Thirteen per cent of recent graduates presented for treatment at least once each year during their tenure as students, 57 per cent sought care in more than one year, and 24 per cent did not seek care at all. It was concluded that factors other than cost, accessibility, and educational level operate to prevent total utilization of prepaid dental health care in a young adult age group.", "contents": "Utilization of prepaid dental health care by students in health professional schools. Utilization of prepaid dental health care was studied, involving health professional students in an open panel program at the University of Alabama Medical Center. Major barriers to treatment were eliminated and optimal conditions for utilization established. Annual utilization ranged from 44-61 per cent during the period extending from 1969-70 to 1975-76. The majority of enrollees treated sought care from the Student Dental Health Plan (SDHP) clinic, rather than from private practitioners. Thirteen per cent of recent graduates presented for treatment at least once each year during their tenure as students, 57 per cent sought care in more than one year, and 24 per cent did not seek care at all. It was concluded that factors other than cost, accessibility, and educational level operate to prevent total utilization of prepaid dental health care in a young adult age group."}
{"id": "PMID:484755", "title": "Presence of observers at patient-practitioner interactions: impact on coordination of care and methodologic implications.", "content": "In this study in an urban practice, the presence of a neutral observer at follow-up visits enhanced the extent to which practitioners recognized problems which patients had in a previous visit. This improvement was limited to those problems which initially had been mentioned by patients as requiring follow-up. Follow-up of problems initially mentioned by practitioners as needing follow-up was not improved by the observer unless the problem was also mentioned by the patient. Investigators whose information about practitioner-patient interaction depends upon the presence of an observer should be aware of this and possibly other effects. Although routine involvement of a neutral observer in patient-practitioner interactions is probably undesirable, selected deployment of observers or similar alternatives may be useful in situations where practitioner-patient communication is inadequate.", "contents": "Presence of observers at patient-practitioner interactions: impact on coordination of care and methodologic implications. In this study in an urban practice, the presence of a neutral observer at follow-up visits enhanced the extent to which practitioners recognized problems which patients had in a previous visit. This improvement was limited to those problems which initially had been mentioned by patients as requiring follow-up. Follow-up of problems initially mentioned by practitioners as needing follow-up was not improved by the observer unless the problem was also mentioned by the patient. Investigators whose information about practitioner-patient interaction depends upon the presence of an observer should be aware of this and possibly other effects. Although routine involvement of a neutral observer in patient-practitioner interactions is probably undesirable, selected deployment of observers or similar alternatives may be useful in situations where practitioner-patient communication is inadequate."}
{"id": "PMID:484757", "title": "Voluntary coordination as a strategy of plan implementation for health systems agencies.", "content": "Health planning agencies are faced with the difficult mission of guiding change within a large complex social system whose power is dispersed. Initial short- and long-range plans have been established as frameworks, and now the major focus is implementation. Regulation (non-voluntary coordination) and voluntary coordination are the major means of implementation. Voluntary coordination is a significant strategy for consideration by Health Systems Agencies (HSAs). It may interact with regulation as a competitor, substitute, or complement. Because of limited regulatory powers, HSAs are dependent upon voluntary coordination as a major means of influencing behavior. Conflict, a major feature of voluntary coordination, has the potential of being used as a constructive means for dialogue; negotiation and bargaining may become positive approaches to arriving at decisions. Legitimized community authority is the primary source of authority in a strategy dominated by voluntary coordination as contrasted to state or federal mandates in a regulatory strategy. Knowledge of the environment within which the HSA operates will assist HSA staff and board to arrive at rational and realistic decisions.", "contents": "Voluntary coordination as a strategy of plan implementation for health systems agencies. Health planning agencies are faced with the difficult mission of guiding change within a large complex social system whose power is dispersed. Initial short- and long-range plans have been established as frameworks, and now the major focus is implementation. Regulation (non-voluntary coordination) and voluntary coordination are the major means of implementation. Voluntary coordination is a significant strategy for consideration by Health Systems Agencies (HSAs). It may interact with regulation as a competitor, substitute, or complement. Because of limited regulatory powers, HSAs are dependent upon voluntary coordination as a major means of influencing behavior. Conflict, a major feature of voluntary coordination, has the potential of being used as a constructive means for dialogue; negotiation and bargaining may become positive approaches to arriving at decisions. Legitimized community authority is the primary source of authority in a strategy dominated by voluntary coordination as contrasted to state or federal mandates in a regulatory strategy. Knowledge of the environment within which the HSA operates will assist HSA staff and board to arrive at rational and realistic decisions."}
{"id": "PMID:484758", "title": "Hazards in the hospital: educating the workforce through its union.", "content": "The hospital workforce is exposed to many occupational hazards of which most hospital workers are unaware. The author and another health educator, both employed with the California State Department of Health, conducted occupational health and safety educational sessions for the membership of a hospital workers' union. These sessions taught nonprofessional workers how to recognize hospital hazards, how to eliminate or minimize these hazards, and informed workers of their legal rights under the California Occupational Safety and Health Act. We first had to convince the union health and safety officer that we had skills and information which would be of benefit to the union. Once that hurdle was crossed, we encountered some difficulty conducting an adequate needs assessment and including the target population in the planning of the educationals. Two series of educational sessions for rank-and-file members were conducted and were enthusiastically received by those attending, with a request for more sessions.", "contents": "Hazards in the hospital: educating the workforce through its union. The hospital workforce is exposed to many occupational hazards of which most hospital workers are unaware. The author and another health educator, both employed with the California State Department of Health, conducted occupational health and safety educational sessions for the membership of a hospital workers' union. These sessions taught nonprofessional workers how to recognize hospital hazards, how to eliminate or minimize these hazards, and informed workers of their legal rights under the California Occupational Safety and Health Act. We first had to convince the union health and safety officer that we had skills and information which would be of benefit to the union. Once that hurdle was crossed, we encountered some difficulty conducting an adequate needs assessment and including the target population in the planning of the educationals. Two series of educational sessions for rank-and-file members were conducted and were enthusiastically received by those attending, with a request for more sessions."}
{"id": "PMID:484759", "title": "Blizzard morbidity and mortality: Rhode Island, 1978.", "content": "The total number of outpatient visits to 10 hospital emergency rooms declined 64 per cent and emergency admissions 35 per cent immediately after the February 1978 blizzard in Rhode Island; both quickly returned to normal levels. Three days after the storm, admissions for myocardial infarction increased markedly. Total mortality and ischemic heart disease deaths showed a considerable increase for the five-day period following the blizzard. The physical and psychological stress of the blizzard probably precipitated cardiac deaths in susceptible individuals.", "contents": "Blizzard morbidity and mortality: Rhode Island, 1978. The total number of outpatient visits to 10 hospital emergency rooms declined 64 per cent and emergency admissions 35 per cent immediately after the February 1978 blizzard in Rhode Island; both quickly returned to normal levels. Three days after the storm, admissions for myocardial infarction increased markedly. Total mortality and ischemic heart disease deaths showed a considerable increase for the five-day period following the blizzard. The physical and psychological stress of the blizzard probably precipitated cardiac deaths in susceptible individuals."}
{"id": "PMID:484760", "title": "Vaccine failures in the epidemiology and control of measles.", "content": "Data were employed from confirmed measles case reports and an immunization survey to estimate the apparent effectiveness of measles vaccine in Los Angeles County in 1976. We evaluated the age-specific proportions of our case load arising from persons erroneously thought to possess immunity. Thirty five per cent of our cases claimed to have had prior measles vaccination, and over 60 per cent of these reported having written records. Since community measles control programs rely on immunization records as the principal screening tool, the shortcomings observed need to be recognized.", "contents": "Vaccine failures in the epidemiology and control of measles. Data were employed from confirmed measles case reports and an immunization survey to estimate the apparent effectiveness of measles vaccine in Los Angeles County in 1976. We evaluated the age-specific proportions of our case load arising from persons erroneously thought to possess immunity. Thirty five per cent of our cases claimed to have had prior measles vaccination, and over 60 per cent of these reported having written records. Since community measles control programs rely on immunization records as the principal screening tool, the shortcomings observed need to be recognized."}
{"id": "PMID:484761", "title": "A vitamin profile of heroin addiction.", "content": "Circulating thiamine, riboflavin, nicotinates, folates, vitamin B12, B6, A, and carotenes of 149 heroin addicts aged 17-60 years were compared to 204 healthy subjects not using drugs or vitamins. Only 24 per cent of the addicts had no evidence of hypovitaminemia; 45 per cent and 37 per cent had vitamin B6 and folate deficit respectively, whereas deficits of thiamine, vitamin B12, riboflavin, and nicotinate were recorded for 13-19 per cent of the addict population; impaired liver function in addicts did not influence these results.", "contents": "A vitamin profile of heroin addiction. Circulating thiamine, riboflavin, nicotinates, folates, vitamin B12, B6, A, and carotenes of 149 heroin addicts aged 17-60 years were compared to 204 healthy subjects not using drugs or vitamins. Only 24 per cent of the addicts had no evidence of hypovitaminemia; 45 per cent and 37 per cent had vitamin B6 and folate deficit respectively, whereas deficits of thiamine, vitamin B12, riboflavin, and nicotinate were recorded for 13-19 per cent of the addict population; impaired liver function in addicts did not influence these results."}
{"id": "PMID:484765", "title": "The psychological effects of differential treatment of a high risk sample in a randomized clinical trial.", "content": "A study was carried out using 616 participants in a randomized clinical trial at the Harvard MRFIT (Multiple Risk Factor Intervention Trial) Clinical Center, to test if there were differences in the psychological dimensions of anxiety, depression, and functional heart symptoms in groups given different levels of treatment in a CHD (Coronary Heart Disease) Intervention Program. A theoretical framework was given to justify a number of hypotheses as to the induction of adverse psychological effects. At the end of two years in the MRFIT Program there were no significant differences between the special intervention group (SI) and the usual care group (UC) in the selected psychological variables.", "contents": "The psychological effects of differential treatment of a high risk sample in a randomized clinical trial. A study was carried out using 616 participants in a randomized clinical trial at the Harvard MRFIT (Multiple Risk Factor Intervention Trial) Clinical Center, to test if there were differences in the psychological dimensions of anxiety, depression, and functional heart symptoms in groups given different levels of treatment in a CHD (Coronary Heart Disease) Intervention Program. A theoretical framework was given to justify a number of hypotheses as to the induction of adverse psychological effects. At the end of two years in the MRFIT Program there were no significant differences between the special intervention group (SI) and the usual care group (UC) in the selected psychological variables."}
{"id": "PMID:484766", "title": "Influence of parasitism on secretin-inhibited gastric secretion.", "content": "This study tested the hypothesis that the inhibitory action of secretin on gastrin-stimulated gastric acid and pepsin secretion is comprised in animals harboring intestinal stages of the parasite Trichinella spiralis. Pentagastrin-stimulated acid and pepsin secretion, and the influence of secretin on these processes, were measured in dogs prepared with gastric fistulas and Heidenhain pouches. Dogs were studied before and after infection with 10(4) T. spiralis larvae/kg body weight. Gastric secretion was stimulated by constant intravenous infusion of pentagastrin, 1 microgram/kg per hour. Exogenous secretin inhibited pentagastrin-stimulated acid and pepsin output from both the main stomach and Heidenhain pouch in infected as well as in the uninfected dogs. Identical inhibition was observed in uninfected dogs during duodenal infusion with HCl to release endogenous secretin. In contrast, duodenal stimulation with HCl did not inhibit acid and pepsin secretion in dogs tested during the 1st week following infection. Results support the conclusion that the regulatory effect of secretin on gastrin-stimulated gastric secretion is impaired during the early phase of infection, and is due to depressed synthesis or release of secretin from duodenal mucosa.", "contents": "Influence of parasitism on secretin-inhibited gastric secretion. This study tested the hypothesis that the inhibitory action of secretin on gastrin-stimulated gastric acid and pepsin secretion is comprised in animals harboring intestinal stages of the parasite Trichinella spiralis. Pentagastrin-stimulated acid and pepsin secretion, and the influence of secretin on these processes, were measured in dogs prepared with gastric fistulas and Heidenhain pouches. Dogs were studied before and after infection with 10(4) T. spiralis larvae/kg body weight. Gastric secretion was stimulated by constant intravenous infusion of pentagastrin, 1 microgram/kg per hour. Exogenous secretin inhibited pentagastrin-stimulated acid and pepsin output from both the main stomach and Heidenhain pouch in infected as well as in the uninfected dogs. Identical inhibition was observed in uninfected dogs during duodenal infusion with HCl to release endogenous secretin. In contrast, duodenal stimulation with HCl did not inhibit acid and pepsin secretion in dogs tested during the 1st week following infection. Results support the conclusion that the regulatory effect of secretin on gastrin-stimulated gastric secretion is impaired during the early phase of infection, and is due to depressed synthesis or release of secretin from duodenal mucosa."}
{"id": "PMID:484767", "title": "Damage to Trichinella spiralis newborn larvae by eosinophil major basic protein.", "content": "Purified eosinophil major basic protein damaged newborn larvae of Trichinella siralis when added to in vitro cultures. Damaged larvae were stiffened, immobile, and showed a granular appearance by light microscopy. Larvae in control cultures and in cultures containing equimolar concentrations of other basic proteins failed to exhibit such damage.", "contents": "Damage to Trichinella spiralis newborn larvae by eosinophil major basic protein. Purified eosinophil major basic protein damaged newborn larvae of Trichinella siralis when added to in vitro cultures. Damaged larvae were stiffened, immobile, and showed a granular appearance by light microscopy. Larvae in control cultures and in cultures containing equimolar concentrations of other basic proteins failed to exhibit such damage."}
{"id": "PMID:484768", "title": "Schistosomiasis haematobia in coast province Kenya. Relationship between egg output and morbidity.", "content": "Several studies of schistosomiasis haematobia in Africa have revealed a correlation between intensity of infection as measured by urine egg counts and severity of disease as determined by intravenous pyelography. The present study consisted of a survey of 390 school children in the coastal area of Kenya involving a single egg count, and intravenous pyelograms in a stratified random sample of 69 children; the results showed a greater prevalence of urinary tract disease in those with higher intensities of infection. This survey was then followed by a more detailed study in which nine consecutive daily egg counts were done on 121 children; 17 of these children, subdivided into three groups with different intensities in infection, were given intravenous pyelograms. The results were similar in the 11 children with minimal and moderate counts (averaging, respectively, less than 1 egg and 167 eggs/10 ml urine daily), with approximately 30% having bladder or renal abnormalities. In comparison, all of the six children with heavy counts (averaging 1,288 eggs/10 ml urine daily) had bladder lesions and five of them had renal lesions.", "contents": "Schistosomiasis haematobia in coast province Kenya. Relationship between egg output and morbidity. Several studies of schistosomiasis haematobia in Africa have revealed a correlation between intensity of infection as measured by urine egg counts and severity of disease as determined by intravenous pyelography. The present study consisted of a survey of 390 school children in the coastal area of Kenya involving a single egg count, and intravenous pyelograms in a stratified random sample of 69 children; the results showed a greater prevalence of urinary tract disease in those with higher intensities of infection. This survey was then followed by a more detailed study in which nine consecutive daily egg counts were done on 121 children; 17 of these children, subdivided into three groups with different intensities in infection, were given intravenous pyelograms. The results were similar in the 11 children with minimal and moderate counts (averaging, respectively, less than 1 egg and 167 eggs/10 ml urine daily), with approximately 30% having bladder or renal abnormalities. In comparison, all of the six children with heavy counts (averaging 1,288 eggs/10 ml urine daily) had bladder lesions and five of them had renal lesions."}
{"id": "PMID:484769", "title": "Ovaries and adrenals in murine Schistosomiasis mansoni. III. Morphology and function of the adrenals in acute infection.", "content": "In mice with acute Schistosoma mansoni infection lipoid depletion was found in the zona fasciculata of the adrenal cortex without any other major histological alterations of the adrenals. Schistosomiasis did not influence the weight of the adrenals, or the cortisol level of the blood serum as determined by radioimmunoassay. The changes in morphology and function of the ovaries in the same infected animals reported earlier point to the vulnerability of the gonads in schistosomiasis mansoni, which may be a consequence of partial hypopituitarism.", "contents": "Ovaries and adrenals in murine Schistosomiasis mansoni. III. Morphology and function of the adrenals in acute infection. In mice with acute Schistosoma mansoni infection lipoid depletion was found in the zona fasciculata of the adrenal cortex without any other major histological alterations of the adrenals. Schistosomiasis did not influence the weight of the adrenals, or the cortisol level of the blood serum as determined by radioimmunoassay. The changes in morphology and function of the ovaries in the same infected animals reported earlier point to the vulnerability of the gonads in schistosomiasis mansoni, which may be a consequence of partial hypopituitarism."}
{"id": "PMID:484770", "title": "Resistance to tick-borne Francisella tularensis by tick-sensitized rabbits: allergic klendusity.", "content": "Mammals become hypersensitive to ticks that feed upon them. That hypersensitivity was thought responsible for an observation that a large number of Francisella tularensis-infected Dermacentor variabilis failed to infect a rabbit previously exposed to ticks of that species. In a series of tests of that hypothesis, rabbits sensitized to ticks were often significantly more resistant than control animals to tick-borne tularemia. The conditions that determine the klendusity are thought to be variable and complex but the phenomenon must be of importance in the epidemiology of some arthropod-borne agents.", "contents": "Resistance to tick-borne Francisella tularensis by tick-sensitized rabbits: allergic klendusity. Mammals become hypersensitive to ticks that feed upon them. That hypersensitivity was thought responsible for an observation that a large number of Francisella tularensis-infected Dermacentor variabilis failed to infect a rabbit previously exposed to ticks of that species. In a series of tests of that hypothesis, rabbits sensitized to ticks were often significantly more resistant than control animals to tick-borne tularemia. The conditions that determine the klendusity are thought to be variable and complex but the phenomenon must be of importance in the epidemiology of some arthropod-borne agents."}
{"id": "PMID:484771", "title": "Higher venereal infection and transmission rates with La Crosse virus in Aedes triseriatus engorged before mating.", "content": "Infection of colonized female Aedes triseriatus by La Crosse (LAC) virus occurred more frequently when females were inseminated by infected males after the females engorged blood (49% of 39) than when mating took place before engorgement (4% of 554). Salivary transmission of LAC virus to mice also was more frequent in females venerally infected after engorgement on a normal mouse (35% of 34) than in females mated before engorgement (2% of 49). LAC virus was transovarially transmitted by 40% of 10 females mated by infected males, and in 64% of 279 progeny reared from eggs of second or later ovarian cycles.", "contents": "Higher venereal infection and transmission rates with La Crosse virus in Aedes triseriatus engorged before mating. Infection of colonized female Aedes triseriatus by La Crosse (LAC) virus occurred more frequently when females were inseminated by infected males after the females engorged blood (49% of 39) than when mating took place before engorgement (4% of 554). Salivary transmission of LAC virus to mice also was more frequent in females venerally infected after engorgement on a normal mouse (35% of 34) than in females mated before engorgement (2% of 49). LAC virus was transovarially transmitted by 40% of 10 females mated by infected males, and in 64% of 279 progeny reared from eggs of second or later ovarian cycles."}
{"id": "PMID:484772", "title": "Aedes triseriatus and La Crosse virus: lack of infection in eggs of the first ovarian cycle following oral infection of females.", "content": "La Crosse (LAC) virus filial infection rates were 0% for 279 first ovarian cycle larvae, 43% for 380 second ovarian cycle larvae, and 58% for 363 third ovarian cycle larvae from orally infected mosquitoes representing 14 Wisconsin populations of Aedes triseriatus. LAC virus was not detected in 72 pools representing 2,250 first ovarian cycle larvae, while 35 pools and 16 pools each containing 30 second and third ovarian cycle larvae, respectively, were all positive for LAC virus. Similar results were obtained when the extrinsic incubation period temperature was 25 degrees C, 27 degrees C, or variable (17, 23, and 29 degrees C for 8 hours each). LAC virus was not detected in 240 second ovarian cycle larvae in which the bloodmeal for the first ovarian cycle was non-infectious. Infection was not detected in 337 first ovarian cycle larvae from female mosquitoes that had been injected intrathoracically with LAC virus concomitantly with receiving a non-infectious bloodmeal. After an extrinsic incubation temperature of 25 degrees C, LAC virus was discovered in dissected mosquito ovarian tissue 7 days postfeeding on an infectious bloodmeal. The epidemiological implications of these findings are discussed.", "contents": "Aedes triseriatus and La Crosse virus: lack of infection in eggs of the first ovarian cycle following oral infection of females. La Crosse (LAC) virus filial infection rates were 0% for 279 first ovarian cycle larvae, 43% for 380 second ovarian cycle larvae, and 58% for 363 third ovarian cycle larvae from orally infected mosquitoes representing 14 Wisconsin populations of Aedes triseriatus. LAC virus was not detected in 72 pools representing 2,250 first ovarian cycle larvae, while 35 pools and 16 pools each containing 30 second and third ovarian cycle larvae, respectively, were all positive for LAC virus. Similar results were obtained when the extrinsic incubation period temperature was 25 degrees C, 27 degrees C, or variable (17, 23, and 29 degrees C for 8 hours each). LAC virus was not detected in 240 second ovarian cycle larvae in which the bloodmeal for the first ovarian cycle was non-infectious. Infection was not detected in 337 first ovarian cycle larvae from female mosquitoes that had been injected intrathoracically with LAC virus concomitantly with receiving a non-infectious bloodmeal. After an extrinsic incubation temperature of 25 degrees C, LAC virus was discovered in dissected mosquito ovarian tissue 7 days postfeeding on an infectious bloodmeal. The epidemiological implications of these findings are discussed."}
{"id": "PMID:484773", "title": "Sterlizing and packaging males of Anopheles Albimanus Wiedemann for field release.", "content": "Technology was developed for sterilizing and packaging as many as 1 million male Anopheles albimanus Wiedemann per day for field release. The average sterility produced in the males during a 1.5-year period was 98.9%. Mortality due to transport of the males in specially developed cages averaged 4.5% for 72-hour-old adults and 2.5% for 24-hour-old adults. The average mortality due to transport and field exposure of pupae was 1.2 to 9.8%, depending on the technique used.", "contents": "Sterlizing and packaging males of Anopheles Albimanus Wiedemann for field release. Technology was developed for sterilizing and packaging as many as 1 million male Anopheles albimanus Wiedemann per day for field release. The average sterility produced in the males during a 1.5-year period was 98.9%. Mortality due to transport of the males in specially developed cages averaged 4.5% for 72-hour-old adults and 2.5% for 24-hour-old adults. The average mortality due to transport and field exposure of pupae was 1.2 to 9.8%, depending on the technique used."}
{"id": "PMID:484774", "title": "Dermatitis caused by cercariae of Orientobilharzia turkestanicum in the Caspian Sea area of Iran.", "content": "Cercarial dermatitis is very common among rice farmers in the Caspian Sea area of Iran. The characteristic eggs of Orientobilharzia turkestanicum were found in the feces of a plowing bull. Fifty-six percent of uninfected Lymnaea gedrosiana maintained at 24-25 degrees C produced cercariae, identical to those from natural infections, 21 days after exposure to the miracidia obtained from the feces of the bull. Cercariae of O. turkestanicum from naturally infected L. gedrosiana produced dermatitis on the arm of one of us.", "contents": "Dermatitis caused by cercariae of Orientobilharzia turkestanicum in the Caspian Sea area of Iran. Cercarial dermatitis is very common among rice farmers in the Caspian Sea area of Iran. The characteristic eggs of Orientobilharzia turkestanicum were found in the feces of a plowing bull. Fifty-six percent of uninfected Lymnaea gedrosiana maintained at 24-25 degrees C produced cercariae, identical to those from natural infections, 21 days after exposure to the miracidia obtained from the feces of the bull. Cercariae of O. turkestanicum from naturally infected L. gedrosiana produced dermatitis on the arm of one of us."}
{"id": "PMID:484776", "title": "Biological control of schistosome transmission in flowing water habitats.", "content": "Marisa cornuarietis was evaluated in Puerto Rico for control of schistosome transmission in flowing water. A population of Biomphalaria glabrata and their schistosome infections disappeared after introduction of 20,000 M. cornuarietis to an endemic stream, while in nearby untreated streams the B. glabrata population remained stable and the schistosome prevalence increased. This method cost U.S. $0.10 per capita for over a year of protection, 5%-10% of the cost of chemical control.", "contents": "Biological control of schistosome transmission in flowing water habitats. Marisa cornuarietis was evaluated in Puerto Rico for control of schistosome transmission in flowing water. A population of Biomphalaria glabrata and their schistosome infections disappeared after introduction of 20,000 M. cornuarietis to an endemic stream, while in nearby untreated streams the B. glabrata population remained stable and the schistosome prevalence increased. This method cost U.S. $0.10 per capita for over a year of protection, 5%-10% of the cost of chemical control."}
{"id": "PMID:484777", "title": "Cultivation of dengue virus type 2 in baby hamster kidney cells in serum-free medium.", "content": "Propagation of dengue virus type 2 (New Guinea C strain) was performed using a shaker culture of baby hamster kidney cells (BHK-21) cultivated in serum-free modified Waymouth medium. Maximum virus titer varied from 10(8.3) to 10(8.8) plaque forming units/ml after incubation of BHK-21 cells in suspension culture at 37 dgrees C for 40-48 hours post-infection.", "contents": "Cultivation of dengue virus type 2 in baby hamster kidney cells in serum-free medium. Propagation of dengue virus type 2 (New Guinea C strain) was performed using a shaker culture of baby hamster kidney cells (BHK-21) cultivated in serum-free modified Waymouth medium. Maximum virus titer varied from 10(8.3) to 10(8.8) plaque forming units/ml after incubation of BHK-21 cells in suspension culture at 37 dgrees C for 40-48 hours post-infection."}
{"id": "PMID:484781", "title": "Plexiform neurofibroma of the head and neck.", "content": "Ten patients with plexiform neurofibroma of the head and neck were observed at M.D. Anderson Hospital between 1956 and 1978. The clinical presentation and the long-term follow-up of the most interesting cases are presented. This is a chronic disease that causes cosmetic and functional deformity because of the size or the position of the tumor, or both. No patient exhibited malignant transformation. Because all of the disease cannot be removed, the surgical procedures should not be radical but should be designed to relieve symptoms or improve cosmesis.", "contents": "Plexiform neurofibroma of the head and neck. Ten patients with plexiform neurofibroma of the head and neck were observed at M.D. Anderson Hospital between 1956 and 1978. The clinical presentation and the long-term follow-up of the most interesting cases are presented. This is a chronic disease that causes cosmetic and functional deformity because of the size or the position of the tumor, or both. No patient exhibited malignant transformation. Because all of the disease cannot be removed, the surgical procedures should not be radical but should be designed to relieve symptoms or improve cosmesis."}
{"id": "PMID:484783", "title": "Feasibility of total thyroidectomy in the treatment of thyroid carcinoma: postoperative radioactive iodine evaluation of 140 cases.", "content": "The objective of this study was to evaluate our experience with so-called total thyroidectomy. Fifty cases (the retrospective group) had been operated on before the start of the study and had been on thyroid replacement therapy; this was discontinued, thyroid-stimulating hormone was administered, and iodine-131 studies were performed. Ninety cases (the prospective group) were evaluated 3 or more weeks postoperatively with no thyroid hormone having been administered. All patients were given 50 microcuries of iodine-131 and uptake studies were performed 24 hours later. All the patients were evaluated at a time when it was believed that practically all circulating hormone secreted by the thyroid gland before its removal had been metabolized or excreted from the body. If the patients with iodine-131 uptakes only in the region of the pyramidal lobe are considered to have had total thyroidectomy, we find that 85.7% of the patients studied had an uptake of less than 1.5%, and 96.4% had an uptake of less than 2.5%. There is increasing evidence to indicate that total thyroidectomy is the procedure of choice in the treatment of differentiated thyroid carcinoma. The present study indicates that with proper technique, total resection of the thyroid gland is feasible in nearly all cases of thyroid carcinoma.", "contents": "Feasibility of total thyroidectomy in the treatment of thyroid carcinoma: postoperative radioactive iodine evaluation of 140 cases. The objective of this study was to evaluate our experience with so-called total thyroidectomy. Fifty cases (the retrospective group) had been operated on before the start of the study and had been on thyroid replacement therapy; this was discontinued, thyroid-stimulating hormone was administered, and iodine-131 studies were performed. Ninety cases (the prospective group) were evaluated 3 or more weeks postoperatively with no thyroid hormone having been administered. All patients were given 50 microcuries of iodine-131 and uptake studies were performed 24 hours later. All the patients were evaluated at a time when it was believed that practically all circulating hormone secreted by the thyroid gland before its removal had been metabolized or excreted from the body. If the patients with iodine-131 uptakes only in the region of the pyramidal lobe are considered to have had total thyroidectomy, we find that 85.7% of the patients studied had an uptake of less than 1.5%, and 96.4% had an uptake of less than 2.5%. There is increasing evidence to indicate that total thyroidectomy is the procedure of choice in the treatment of differentiated thyroid carcinoma. The present study indicates that with proper technique, total resection of the thyroid gland is feasible in nearly all cases of thyroid carcinoma."}
{"id": "PMID:484786", "title": "Tumors arising in accessory parotid tissue.", "content": "A summary is presented of our experience with 23 patients who had accessory parotid tumors, comprising 1% of all primary parotid neoplasms seen during a 40 year period. Although more than half of the tumors proved malignant, adequate excision was usually curative. We prefer to approach these tumors through an extended cheek-flap incision. Once the lesion is exposed, either local excision or resection in conjunction with subtotal parotidectomy can be performed, depending on the clinical findings.", "contents": "Tumors arising in accessory parotid tissue. A summary is presented of our experience with 23 patients who had accessory parotid tumors, comprising 1% of all primary parotid neoplasms seen during a 40 year period. Although more than half of the tumors proved malignant, adequate excision was usually curative. We prefer to approach these tumors through an extended cheek-flap incision. Once the lesion is exposed, either local excision or resection in conjunction with subtotal parotidectomy can be performed, depending on the clinical findings."}
{"id": "PMID:484789", "title": "Use of surgery and postoperative radiation in the treatment of advanced squamous cell carcinoma of the pyriform sinus.", "content": "Forty-seven patients with very advanced cancer of the pyriform sinus were treated with surgery and immediate postoperative radiation in a planned sequential manner. Eradication of the cancer above the clavicles was obtained in 81% of the patients, with a treatment mortality of 8%. Most of the patients' tumors were resected with primary closure, which obviated the need for complicated reconstructive procedures. The survival rate of 23% at 2 years and the high rate of distant metastasis reflect the aggressive biologic behavior of this cancer. The need to find an adjuvant treatment that will act upon the systemic spread of this cancer is obvious.", "contents": "Use of surgery and postoperative radiation in the treatment of advanced squamous cell carcinoma of the pyriform sinus. Forty-seven patients with very advanced cancer of the pyriform sinus were treated with surgery and immediate postoperative radiation in a planned sequential manner. Eradication of the cancer above the clavicles was obtained in 81% of the patients, with a treatment mortality of 8%. Most of the patients' tumors were resected with primary closure, which obviated the need for complicated reconstructive procedures. The survival rate of 23% at 2 years and the high rate of distant metastasis reflect the aggressive biologic behavior of this cancer. The need to find an adjuvant treatment that will act upon the systemic spread of this cancer is obvious."}
{"id": "PMID:484790", "title": "Carcinoma of the lip.", "content": "(1) Carcinoma of the lip occurs predominantly in men in their seventh and eighth decades of life. (2) Epidermoid carcinoma is the most common malignant lip tumor. (3) Metastases to cervical lymph nodes are uncommon. (4) Elective neck dissection is not indicated. (5) Therapeutic radical neck dissection can benefit the patient in the presence of proved suprahyoid lymph node metastases. (6) The five year survival rate for epidermoid carcinoma of the lip is higher than the rate for epidermoid carcinoma of other sites in the oral cavity or pharynx. (7) Local recurrence does not correlate with the size of the primary tumor. (8) Initial treatment failures can frequently be salvaged surgically.", "contents": "Carcinoma of the lip. (1) Carcinoma of the lip occurs predominantly in men in their seventh and eighth decades of life. (2) Epidermoid carcinoma is the most common malignant lip tumor. (3) Metastases to cervical lymph nodes are uncommon. (4) Elective neck dissection is not indicated. (5) Therapeutic radical neck dissection can benefit the patient in the presence of proved suprahyoid lymph node metastases. (6) The five year survival rate for epidermoid carcinoma of the lip is higher than the rate for epidermoid carcinoma of other sites in the oral cavity or pharynx. (7) Local recurrence does not correlate with the size of the primary tumor. (8) Initial treatment failures can frequently be salvaged surgically."}
{"id": "PMID:484791", "title": "Carotid arterial hemorrhage after radical head and neck surgery.", "content": "From 1960 to 1974, 63 patients treated by the Head and Neck Service of Memorial Hospital underwent carotid arterial ligation for control of actual or threatened carotid rupture. Twenty-one of these patients died without leaving the hospital. Fourteen of these deaths were the direct result of carotid ligation or hemorrhage. Five of the surviving patients suffered permanent neurologic damage. Factors predisposing to carotid rupture included a history of radiation, advanced disease, the presence of an oral or pharyngeal suture line in continuity with a dissected neck, and the postoperative development of skin necrosis or fistulas. Because elective carotid ligation did not prevent the consequences of carotid rupture and because reestablishment of vascular continuity is almost never possible, all attempts should be made to avoid the conditions that are precursors to this most serious complication of radical head and neck surgery.", "contents": "Carotid arterial hemorrhage after radical head and neck surgery. From 1960 to 1974, 63 patients treated by the Head and Neck Service of Memorial Hospital underwent carotid arterial ligation for control of actual or threatened carotid rupture. Twenty-one of these patients died without leaving the hospital. Fourteen of these deaths were the direct result of carotid ligation or hemorrhage. Five of the surviving patients suffered permanent neurologic damage. Factors predisposing to carotid rupture included a history of radiation, advanced disease, the presence of an oral or pharyngeal suture line in continuity with a dissected neck, and the postoperative development of skin necrosis or fistulas. Because elective carotid ligation did not prevent the consequences of carotid rupture and because reestablishment of vascular continuity is almost never possible, all attempts should be made to avoid the conditions that are precursors to this most serious complication of radical head and neck surgery."}
{"id": "PMID:484792", "title": "Sublabial approach to the nasal and nasopharyngeal cavities.", "content": "A sublabial transnasal degloving approach to the mid-face, nasal cavity, septum, paranasal sinuses, and nasopharynx is described. This approach provides adequate exposure and ample facility for management of tumors in these areas. The pterygomaxillary space and infratemporal fossa ard readily accessible for hemostasis and management of tumor extensions. Children, adolescents, and women with benign and low-grade malignant tumors of these areas are the ideal candidates for this procedure. Twenty-six patients have been managed in this fashion, with no major complication. The cosmetic results have been excellent. This approach to the mid-third of the face, nasal, paranasal, and nasopharyngeal cavities should receive more attention and use.", "contents": "Sublabial approach to the nasal and nasopharyngeal cavities. A sublabial transnasal degloving approach to the mid-face, nasal cavity, septum, paranasal sinuses, and nasopharynx is described. This approach provides adequate exposure and ample facility for management of tumors in these areas. The pterygomaxillary space and infratemporal fossa ard readily accessible for hemostasis and management of tumor extensions. Children, adolescents, and women with benign and low-grade malignant tumors of these areas are the ideal candidates for this procedure. Twenty-six patients have been managed in this fashion, with no major complication. The cosmetic results have been excellent. This approach to the mid-third of the face, nasal, paranasal, and nasopharyngeal cavities should receive more attention and use."}
{"id": "PMID:484793", "title": "Construction of a neolarynx after radiation and laryngectomy.", "content": "We have developed a technique for forming a new larynx from a cervical \"bucket handle\" flap to replace the larynx after laryngectomy in patients who have had radiation of the neck. Experience with the technique in six patients suggests that it is more successful than previous procedures used in such patients and may offer some advantages as a general technique of laryngeal construction. The procedure is designed to preserve speech and swallowing without aspiration, but translaryngeal breathing is sacrificed and respiration requires a permanent tracheostomy tube.", "contents": "Construction of a neolarynx after radiation and laryngectomy. We have developed a technique for forming a new larynx from a cervical \"bucket handle\" flap to replace the larynx after laryngectomy in patients who have had radiation of the neck. Experience with the technique in six patients suggests that it is more successful than previous procedures used in such patients and may offer some advantages as a general technique of laryngeal construction. The procedure is designed to preserve speech and swallowing without aspiration, but translaryngeal breathing is sacrificed and respiration requires a permanent tracheostomy tube."}
{"id": "PMID:484812", "title": "Anaesthesia and the Valsalva manoeuvre. A test of circulatory responses in neurosurgical patients including those in the sitting position.", "content": "The four phases of the Valsalva manoeuvre were studied before and after anaesthesia had been induced with a technique of either spontaneous breathing or controlled ventilation of the lungs. The Valsalva manoeuvre was found to be an unreliable predictor of a patient's ability to tolerate the upright or sitting position for neurosurgical procedures. However, a false negative response is more likely to occur than a false positive response.", "contents": "Anaesthesia and the Valsalva manoeuvre. A test of circulatory responses in neurosurgical patients including those in the sitting position. The four phases of the Valsalva manoeuvre were studied before and after anaesthesia had been induced with a technique of either spontaneous breathing or controlled ventilation of the lungs. The Valsalva manoeuvre was found to be an unreliable predictor of a patient's ability to tolerate the upright or sitting position for neurosurgical procedures. However, a false negative response is more likely to occur than a false positive response."}
{"id": "PMID:484813", "title": "The measurement of occlusion pressure during anaesthesia. A comparison of the depression of respiratory drive by methohexitone and etomidate.", "content": "Occlusion pressure (Po max) was used to indicate the depression of respiratory drive following rapid administration of methohexitone 0.5 mg/kg and etomidate 0.067 mg/kg to fourteen patients under stable light anaesthesia. Methohexitone produced considerably more respiratory depression than etomidate, the difference probably being clinically significant. Po max is the maximum sub-atmospheric pressure generated in the trachea when inspiration is prevented by occlusion of the airway, at functional residual capacity. The factors concerning the use of this simple, non-invasive technique during anaesthesia are discussed, and suggestions made for producing consistent, useful, measurements.", "contents": "The measurement of occlusion pressure during anaesthesia. A comparison of the depression of respiratory drive by methohexitone and etomidate. Occlusion pressure (Po max) was used to indicate the depression of respiratory drive following rapid administration of methohexitone 0.5 mg/kg and etomidate 0.067 mg/kg to fourteen patients under stable light anaesthesia. Methohexitone produced considerably more respiratory depression than etomidate, the difference probably being clinically significant. Po max is the maximum sub-atmospheric pressure generated in the trachea when inspiration is prevented by occlusion of the airway, at functional residual capacity. The factors concerning the use of this simple, non-invasive technique during anaesthesia are discussed, and suggestions made for producing consistent, useful, measurements."}
{"id": "PMID:484814", "title": "Relief of pain after surgery. A comparison of sublingual buprenorphine and intramuscular papaveretum.", "content": "Sublingual buprenorphine (0.4 mg) and intramuscular papaveretum (20 mg) were compared in sixty patients after abdominal hysterectomy. Though slower in onset of effect the sublingual tablets proved effective for pain relief and appeared to have a longer duration of action. The only side-effects of note were nausea and vomiting which occurred after both treatments. Haloperidol was tried as a long acting antiemetic and appeared successful.", "contents": "Relief of pain after surgery. A comparison of sublingual buprenorphine and intramuscular papaveretum. Sublingual buprenorphine (0.4 mg) and intramuscular papaveretum (20 mg) were compared in sixty patients after abdominal hysterectomy. Though slower in onset of effect the sublingual tablets proved effective for pain relief and appeared to have a longer duration of action. The only side-effects of note were nausea and vomiting which occurred after both treatments. Haloperidol was tried as a long acting antiemetic and appeared successful."}
{"id": "PMID:484815", "title": "Postoperative analgesia after circumcision. A randomized comparison between caudal analgesia and intramuscular morphine in boys.", "content": "A randomised blind comparison between caudal analgesia (1.5 mg/kg bupivacaine) and intramuscular morphine (0.15 mg/kg) is reported. Forty boys were studied. Caudal analgesia is shown to be significantly better than morphine by means of a linear analogue scale designed to quantify behaviour immediately after operation.", "contents": "Postoperative analgesia after circumcision. A randomized comparison between caudal analgesia and intramuscular morphine in boys. A randomised blind comparison between caudal analgesia (1.5 mg/kg bupivacaine) and intramuscular morphine (0.15 mg/kg) is reported. Forty boys were studied. Caudal analgesia is shown to be significantly better than morphine by means of a linear analogue scale designed to quantify behaviour immediately after operation."}
{"id": "PMID:484816", "title": "The effects of ketamine on the canine coronary circulation.", "content": "Systemic and coronary haemodynamic measurements have been made in six healthy greyhounds anaesthetized with trichloroethylene. The administration of ketamine in bolus doses of 5 mg/kg, 10 mg/kg and 5 mg/kg followed by an infusion 0.1 mg/kg/min was found to be accompanied by a decrease in arterial pressure and an increase in cardiac output produced by an increase (84%) in stroke volume. Coronary blood flow increased greatly as did myocardial oxygen consumption and there was no change in myocardial oxygen extraction.", "contents": "The effects of ketamine on the canine coronary circulation. Systemic and coronary haemodynamic measurements have been made in six healthy greyhounds anaesthetized with trichloroethylene. The administration of ketamine in bolus doses of 5 mg/kg, 10 mg/kg and 5 mg/kg followed by an infusion 0.1 mg/kg/min was found to be accompanied by a decrease in arterial pressure and an increase in cardiac output produced by an increase (84%) in stroke volume. Coronary blood flow increased greatly as did myocardial oxygen consumption and there was no change in myocardial oxygen extraction."}
{"id": "PMID:484817", "title": "Detection of venous air embolism. A clinical report using end-tidal carbon dioxide monitoring during neurosurgery.", "content": "A clinical report on the detection of venous air embolism during neurosurgery is presented. The use of end-tidal CO2 monitoring is described.", "contents": "Detection of venous air embolism. A clinical report using end-tidal carbon dioxide monitoring during neurosurgery. A clinical report on the detection of venous air embolism during neurosurgery is presented. The use of end-tidal CO2 monitoring is described."}
{"id": "PMID:484818", "title": "Complications of guided blind endotracheal intubation.", "content": "The complications observed in twelve patients undergoing guided blind nasotracheal intubation are presented. The commonest was minor bleeding, followed by difficulty in hooking the cannula out of the nostril. These complications are minor enough to justify the more frequent use of the technique in patients with intubation problems.", "contents": "Complications of guided blind endotracheal intubation. The complications observed in twelve patients undergoing guided blind nasotracheal intubation are presented. The commonest was minor bleeding, followed by difficulty in hooking the cannula out of the nostril. These complications are minor enough to justify the more frequent use of the technique in patients with intubation problems."}
{"id": "PMID:484820", "title": "[Coagulation problems associated with massive blood transfusions. An experimental study (author's transl)].", "content": "During storage banked blood show changes as far as function and concentration of their corpuscular and plasmatic ingredients are concerned. Antihaemophilic globulin (factor VIII) and pro-accelerin (factor V) in particular lose their activities so quickly that only very few activities still remain after a storage period of 3-5 days. In connection with existing primary diseases but also in trauma and shock a dilution-coagulopathy caused by insufficient supply of factors VIII and V can develop during massive transfusion, and this especially with banked blood already stored over a longer period of time. Therapy of this disturbance of coagulation consists of substituting the missing coagulation factors under heparin protection.", "contents": "[Coagulation problems associated with massive blood transfusions. An experimental study (author's transl)]. During storage banked blood show changes as far as function and concentration of their corpuscular and plasmatic ingredients are concerned. Antihaemophilic globulin (factor VIII) and pro-accelerin (factor V) in particular lose their activities so quickly that only very few activities still remain after a storage period of 3-5 days. In connection with existing primary diseases but also in trauma and shock a dilution-coagulopathy caused by insufficient supply of factors VIII and V can develop during massive transfusion, and this especially with banked blood already stored over a longer period of time. Therapy of this disturbance of coagulation consists of substituting the missing coagulation factors under heparin protection."}
{"id": "PMID:484821", "title": "[High doses of thiopental for therapy of post-ischaemic anoxia of the brain. A case report (author's transl)].", "content": "The case of a 7-year-old child is presented, who suffered circulatory arrest during induction of anaesthesia for surgery for a posterior fossa tumour. A brain ischaemia lasting 6 minutes duration had to be assumed. After restoration of circulation, 825 mg ethiopenta were administered in order to ameliorate a possible post-ischaemic anoxia of the brain according to a protocol by Safar [18]. 11 hours after circulatory arrest the child awoke. Except for a more pronounced left sided hemiparesis and paresis of the left n. abducens no additional neurological deficit was observed compared to the neurological status before induction of anaesthesia.", "contents": "[High doses of thiopental for therapy of post-ischaemic anoxia of the brain. A case report (author's transl)]. The case of a 7-year-old child is presented, who suffered circulatory arrest during induction of anaesthesia for surgery for a posterior fossa tumour. A brain ischaemia lasting 6 minutes duration had to be assumed. After restoration of circulation, 825 mg ethiopenta were administered in order to ameliorate a possible post-ischaemic anoxia of the brain according to a protocol by Safar [18]. 11 hours after circulatory arrest the child awoke. Except for a more pronounced left sided hemiparesis and paresis of the left n. abducens no additional neurological deficit was observed compared to the neurological status before induction of anaesthesia."}
{"id": "PMID:484822", "title": "[Pneumothorax caused by anaesthesiological manipulations (author's transl)].", "content": "Pneumothorax may occur following procedures such as the insertion of a subclavian catheter, intercostal blockade, supraclavicular brachial plexus blockade, endotracheal mechanical ventilation or assisted ventilation of the newborn by the \"pulmarca\" method. The incidence of pneumothorax was investigated over a 2 1/2 years period. Measurements to prevent, reduce and treat this complication are discussed and suggestions on the aetiology of bilateral pneumothorax in the newborn are described.", "contents": "[Pneumothorax caused by anaesthesiological manipulations (author's transl)]. Pneumothorax may occur following procedures such as the insertion of a subclavian catheter, intercostal blockade, supraclavicular brachial plexus blockade, endotracheal mechanical ventilation or assisted ventilation of the newborn by the \"pulmarca\" method. The incidence of pneumothorax was investigated over a 2 1/2 years period. Measurements to prevent, reduce and treat this complication are discussed and suggestions on the aetiology of bilateral pneumothorax in the newborn are described."}
{"id": "PMID:484823", "title": "[Functional evaluation of \"tekatest\", a test equipment for halothane-vaporizers (author's transl)].", "content": "The results of investigations on the reliability of a commericially available test equipment for anaesthetic vaporizers based on the Rotameter principle (s.c. \"Tekatest\") are reported. In prototype devices scattered differences up to 27% between the results obtained by the Tekatest and those derived from gas chromatography and mass-spectrometry were noted; in a somewhat improved version an unidirectional error of about 24% became evident. Up to now, therefore, this test equipment cannot be recommended for recalibration of vaporizers in clinical use.", "contents": "[Functional evaluation of \"tekatest\", a test equipment for halothane-vaporizers (author's transl)]. The results of investigations on the reliability of a commericially available test equipment for anaesthetic vaporizers based on the Rotameter principle (s.c. \"Tekatest\") are reported. In prototype devices scattered differences up to 27% between the results obtained by the Tekatest and those derived from gas chromatography and mass-spectrometry were noted; in a somewhat improved version an unidirectional error of about 24% became evident. Up to now, therefore, this test equipment cannot be recommended for recalibration of vaporizers in clinical use."}
{"id": "PMID:484825", "title": "[Effects of dopamine-droperidol interaction on renal function in rats (author's transl)].", "content": "The influence of dopamine and droperidol on the glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) was examined in twelve groups (N = 8) of male wistar rats using the double isotope method with a partially-shielded whole-body counter according to Oberhausen. No alterations of GFR and ERPF could be elicited in normovolaemic rats given dopamine 2--4 micrograns/kg/min (i.v.) alone or in combination with droperidol 0.3 mg/kg. The administration of 10 micrograms/kg/min dopamine alone was followed by a significant (p less than or equal to 0.05) reduction of the ERPF, this effect being abolished by the concomitant use of both drugs. In tourniquet shock dopamine administration or the combined use of dopamine and droperidol produced a significant rise in arterial blood pressure and the renal functions described above. The results in the present study indicate that dopamine infusions will be effective in the presence of froperidol.", "contents": "[Effects of dopamine-droperidol interaction on renal function in rats (author's transl)]. The influence of dopamine and droperidol on the glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) was examined in twelve groups (N = 8) of male wistar rats using the double isotope method with a partially-shielded whole-body counter according to Oberhausen. No alterations of GFR and ERPF could be elicited in normovolaemic rats given dopamine 2--4 micrograns/kg/min (i.v.) alone or in combination with droperidol 0.3 mg/kg. The administration of 10 micrograms/kg/min dopamine alone was followed by a significant (p less than or equal to 0.05) reduction of the ERPF, this effect being abolished by the concomitant use of both drugs. In tourniquet shock dopamine administration or the combined use of dopamine and droperidol produced a significant rise in arterial blood pressure and the renal functions described above. The results in the present study indicate that dopamine infusions will be effective in the presence of froperidol."}
{"id": "PMID:484827", "title": "[Medial AV-nodular rhythm observed during an endolaryngeal micro-surgical intervention (author's transl)].", "content": "A case of therapy resistant junctional nodal rhythm in the course of a microsurgical laryngeal operation is reported. The interaction of possible reflex mechanisms is discussed. Attempts to reverse the electrocardioscopic findings with drugs were unsuccessful. Diminuation of the mechanical irritation caused by the operation-laryngoscope resulted in immediate return to sinusrhythm.", "contents": "[Medial AV-nodular rhythm observed during an endolaryngeal micro-surgical intervention (author's transl)]. A case of therapy resistant junctional nodal rhythm in the course of a microsurgical laryngeal operation is reported. The interaction of possible reflex mechanisms is discussed. Attempts to reverse the electrocardioscopic findings with drugs were unsuccessful. Diminuation of the mechanical irritation caused by the operation-laryngoscope resulted in immediate return to sinusrhythm."}
{"id": "PMID:484829", "title": "[The computerized anaesthetic record of the 'Universit\u00e4tsklinik K\u00f6ln' (author's transl)].", "content": "Some of the main arguments are presented which led us to develop our present anaesthetic record. This record system and a part of its voluminous code is described. The new version provides for the documentation of arbitrarily complicated status, which are due to the preoperative condition of the patient. Furthermore, the course of aneaesthesia and its effects upon the patient can be numerically recorded in a detailed fashion. The punching is affected by a small rate of error only.", "contents": "[The computerized anaesthetic record of the 'Universit\u00e4tsklinik K\u00f6ln' (author's transl)]. Some of the main arguments are presented which led us to develop our present anaesthetic record. This record system and a part of its voluminous code is described. The new version provides for the documentation of arbitrarily complicated status, which are due to the preoperative condition of the patient. Furthermore, the course of aneaesthesia and its effects upon the patient can be numerically recorded in a detailed fashion. The punching is affected by a small rate of error only."}
{"id": "PMID:484870", "title": "[General anesthesia in patients with sickle cell disease (author's transl)].", "content": "From pathogenic datas about sickle cell disease, the authors enumerate precautions that should be taken during pre, per- and post-operative periods. These conclusions have been established according to previous publications and according to a personnel serie of 31 general anaesthesias, 11 of which on patients with sickle cell disease.", "contents": "[General anesthesia in patients with sickle cell disease (author's transl)]. From pathogenic datas about sickle cell disease, the authors enumerate precautions that should be taken during pre, per- and post-operative periods. These conclusions have been established according to previous publications and according to a personnel serie of 31 general anaesthesias, 11 of which on patients with sickle cell disease."}
{"id": "PMID:484872", "title": "[Supra-umbilical laparotomies and perturbed pulmonary blood oxygenation (author's transl)].", "content": "Pulmonary blood flow and ventilation were studied in twenty three patients after supra-umbilical laparotomy by means of samplings of blood gases and expiratory air content, spirometry, and pneumotachography. Although ventilatory perturbations predominate (notably, a lessened tidal volume and an amputation of the residual functional capacity they are found in conjunction with perfusion anomalies, an shown by the study of O2 and CO2 ductances). The PCO2/PO2 diagram of Rahn and Fenn further illustrates these perfusion alterations; in addition, it gives an impression of the metabolic orientation of the patients in the early post-operative period. Several physiopathological notions are derived from the study, as well as some therapeutic proposals.", "contents": "[Supra-umbilical laparotomies and perturbed pulmonary blood oxygenation (author's transl)]. Pulmonary blood flow and ventilation were studied in twenty three patients after supra-umbilical laparotomy by means of samplings of blood gases and expiratory air content, spirometry, and pneumotachography. Although ventilatory perturbations predominate (notably, a lessened tidal volume and an amputation of the residual functional capacity they are found in conjunction with perfusion anomalies, an shown by the study of O2 and CO2 ductances). The PCO2/PO2 diagram of Rahn and Fenn further illustrates these perfusion alterations; in addition, it gives an impression of the metabolic orientation of the patients in the early post-operative period. Several physiopathological notions are derived from the study, as well as some therapeutic proposals."}
{"id": "PMID:484876", "title": "[Humoral and cellular immunity and sepsis in intensive care (author's transl)].", "content": "Different parameters of humoral and cell mediated immunity were studied in 17 patients with sepsis. There is an important decrease of CH 50 (82 p. cent of patients) and variations of serum complement according with activation of alternative pathway in gram negative and gram positive septicemia. The cellular immunity is never normal: the stimulation index of lymphocyte proliferation under the influence of P. H. A. is lower than 3. There is less than 50 p. cent of T lymphocytes forming rosettes. With cure of sepsis a discrimination is observed between patients with transient decreased immunity and those with persistent lower responses.", "contents": "[Humoral and cellular immunity and sepsis in intensive care (author's transl)]. Different parameters of humoral and cell mediated immunity were studied in 17 patients with sepsis. There is an important decrease of CH 50 (82 p. cent of patients) and variations of serum complement according with activation of alternative pathway in gram negative and gram positive septicemia. The cellular immunity is never normal: the stimulation index of lymphocyte proliferation under the influence of P. H. A. is lower than 3. There is less than 50 p. cent of T lymphocytes forming rosettes. With cure of sepsis a discrimination is observed between patients with transient decreased immunity and those with persistent lower responses."}
{"id": "PMID:484877", "title": "[Post-operative necrotising enterocolitis. One case cured by hyperbaric oxygenotherapy (author's transl)].", "content": "About one case of necrotising enterocolitis following an extra-peritoneal and aseptic operation, it seems that a post-operative ileus, by the ischemia of mucous membrane and the swarming of anerobics that it involves, seems to be responsable of this dreadful complication. The macroscopic and deceiver aspect of intestine to an early re-operation can impeed this diagnosis. The hyperbaric oxygenotherapy seems to be a perceptible adjuvant treatment.", "contents": "[Post-operative necrotising enterocolitis. One case cured by hyperbaric oxygenotherapy (author's transl)]. About one case of necrotising enterocolitis following an extra-peritoneal and aseptic operation, it seems that a post-operative ileus, by the ischemia of mucous membrane and the swarming of anerobics that it involves, seems to be responsable of this dreadful complication. The macroscopic and deceiver aspect of intestine to an early re-operation can impeed this diagnosis. The hyperbaric oxygenotherapy seems to be a perceptible adjuvant treatment."}
{"id": "PMID:484879", "title": "[Malignant syndrome in burned children. Nosologic and therapeutic approach].", "content": "A malignant syndrome was observed in 7 children less than 3 years old; the extent of burn was between 5 and 25 p. cent. This syndrome appeared between the second and the fifth days. It was characterized by fever, autonomic disorders and finally coma. The clinical, biological and anatomical features look like the syndrome of Reye. The use of dopamine before coma allowed healing.", "contents": "[Malignant syndrome in burned children. Nosologic and therapeutic approach]. A malignant syndrome was observed in 7 children less than 3 years old; the extent of burn was between 5 and 25 p. cent. This syndrome appeared between the second and the fifth days. It was characterized by fever, autonomic disorders and finally coma. The clinical, biological and anatomical features look like the syndrome of Reye. The use of dopamine before coma allowed healing."}
{"id": "PMID:484880", "title": "[Shock during necrotic amoebic colitis (author's transl)].", "content": "Two varieties of conditions of shock may be isolated during necrotic amoebic colitis (13 cases). Eight patients present a simple hypovolemic shock secondary to wastage by diarrhea and perilesional oedema with globular, protein, alcaline and potassic deficiency. Its prognosis is relatively good, after vascular infilling and corrections of metabolic disorders. Five other patients present real toxi-infectious shock resulting from widespread tissue necrosis with auto-intoxication associated with septicemic complications. Its prognosis is frankly bad. The treatment is far more difficult. The use of cardiovascular analeptics, such as dopamine, after an infilling failure, is not always sufficient to re-establish the situation. The exeresis of necrotic tissues is an indispensable condition to remove the cause of the shock before septicaemic generalisation.", "contents": "[Shock during necrotic amoebic colitis (author's transl)]. Two varieties of conditions of shock may be isolated during necrotic amoebic colitis (13 cases). Eight patients present a simple hypovolemic shock secondary to wastage by diarrhea and perilesional oedema with globular, protein, alcaline and potassic deficiency. Its prognosis is relatively good, after vascular infilling and corrections of metabolic disorders. Five other patients present real toxi-infectious shock resulting from widespread tissue necrosis with auto-intoxication associated with septicemic complications. Its prognosis is frankly bad. The treatment is far more difficult. The use of cardiovascular analeptics, such as dopamine, after an infilling failure, is not always sufficient to re-establish the situation. The exeresis of necrotic tissues is an indispensable condition to remove the cause of the shock before septicaemic generalisation."}
{"id": "PMID:484881", "title": "[Defence of Swan-Ganz catheters during surgical intensive care (author's transl)].", "content": "It is difficult to apprehend certain precarious hemodynamic states with classical parameters alone; the help of a flow-directed pulmonary artery catheter is necessary in order to define the disease accurately and reach a rational therapy. The problem is easily solved by the use of a Swan-Ganz catheter. The authors have selected 50 observations to help them make clear the indications of this method in surgery and traumatology: it appears necessary to use this method not only in specialized surgery such as valvulary surgery under CEC, but also in the treatment and prevention of non-cardiogenic pulmonary oedema and in emergency or bleeding surgery on patients suffering from cardiac disease or renal failure.", "contents": "[Defence of Swan-Ganz catheters during surgical intensive care (author's transl)]. It is difficult to apprehend certain precarious hemodynamic states with classical parameters alone; the help of a flow-directed pulmonary artery catheter is necessary in order to define the disease accurately and reach a rational therapy. The problem is easily solved by the use of a Swan-Ganz catheter. The authors have selected 50 observations to help them make clear the indications of this method in surgery and traumatology: it appears necessary to use this method not only in specialized surgery such as valvulary surgery under CEC, but also in the treatment and prevention of non-cardiogenic pulmonary oedema and in emergency or bleeding surgery on patients suffering from cardiac disease or renal failure."}
{"id": "PMID:484883", "title": "[Hypertensive accident in a surgical patient with unsuspected pheochromocytoma (author's transl)].", "content": "Death may follow anesthetic incident in unsuspected pheochromocytoma. They may lead to hypertensive crisis with cerebrovascular accident and severe arythmias or acute left heart failure with pulmonary edema. We report a case illustrating these problems and in which death has followed. Analysis of the 18 cases recorded in world litterature demonstrates a mortality rate of 85 p. cent. The accident can occur in association with surgery, anesthesia and certain, even minor, investigatory procedures. However if the crisis is recognized and appropriatly treated, the patient may recover before irreversible shock occurs.", "contents": "[Hypertensive accident in a surgical patient with unsuspected pheochromocytoma (author's transl)]. Death may follow anesthetic incident in unsuspected pheochromocytoma. They may lead to hypertensive crisis with cerebrovascular accident and severe arythmias or acute left heart failure with pulmonary edema. We report a case illustrating these problems and in which death has followed. Analysis of the 18 cases recorded in world litterature demonstrates a mortality rate of 85 p. cent. The accident can occur in association with surgery, anesthesia and certain, even minor, investigatory procedures. However if the crisis is recognized and appropriatly treated, the patient may recover before irreversible shock occurs."}
{"id": "PMID:484882", "title": "[Abortion and the use of anaesthesia. Observations after two years' experience (author's transl)].", "content": "The authors give an account of their experience of anaesthesia in 380 abortion cases. Two kinds of technique were used: --paracervical block: 10.2 p. cent of the cases, only one complication; --narco-analgesia: considered to be essential. Dextromoramide was the principal analgesic used, chosen for its pharmacodynamic properties. Three narcotic drugs used successively in conjunction with this were: propanidine, with this drug per-anaesthetic complications and post-operative vomiting proved excessive, C.T. 1341 considerably reduced the frequency of these complications, disodic penthiobarbital did not give rise to any problems.", "contents": "[Abortion and the use of anaesthesia. Observations after two years' experience (author's transl)]. The authors give an account of their experience of anaesthesia in 380 abortion cases. Two kinds of technique were used: --paracervical block: 10.2 p. cent of the cases, only one complication; --narco-analgesia: considered to be essential. Dextromoramide was the principal analgesic used, chosen for its pharmacodynamic properties. Three narcotic drugs used successively in conjunction with this were: propanidine, with this drug per-anaesthetic complications and post-operative vomiting proved excessive, C.T. 1341 considerably reduced the frequency of these complications, disodic penthiobarbital did not give rise to any problems."}
{"id": "PMID:484884", "title": "[The advantages and disadvantages of different methods of access to the internal jugular veins (author's transl)].", "content": "The authors have made a critical study of the respective advantages and disadvantages of the various methods of access to the internal jugular vein. These have been classified under 3 headings: frontal access, lateral access and rear access. They outline their experience of the technique described by Boulanger and Delva in 1977. This method of access seems to be favoured on two scores, its simplicity and the absence of immediate serious complications. These encouraging results lead them to advocate this as the technique they would choose especially in urgent cases.", "contents": "[The advantages and disadvantages of different methods of access to the internal jugular veins (author's transl)]. The authors have made a critical study of the respective advantages and disadvantages of the various methods of access to the internal jugular vein. These have been classified under 3 headings: frontal access, lateral access and rear access. They outline their experience of the technique described by Boulanger and Delva in 1977. This method of access seems to be favoured on two scores, its simplicity and the absence of immediate serious complications. These encouraging results lead them to advocate this as the technique they would choose especially in urgent cases."}
{"id": "PMID:484887", "title": "[Deep hypotension induced by sodium nitroprusside in neurosurgery. I.--Systemic hemodynamic effects (author's transl)].", "content": "The systemic hemodynamic effects of deep hypotension (MAP: 38 +/- 6 mm Hg) induced by sodium nitroprusside (S.N.) were studied in 20 patients who underwent surgery for cerebral aneurysm. The hemodynamic measurements were performed four times.: (1) during the preoperative period, (2) during stable anesthesia just before hypotension, (3) during stable hypotension, (4) 20 minutes after stopping nitroprusside. All patients were mechanically ventilated with a constant tidal volume and rate. Parameters for acid-base balance and Pa O2 were also recorded. Nitroprusside produces arterial and venous dilatation which results in a decrease of afterload and preload. The mean dosage of S. N. was 18 mcg/kg/mn. Systemic vascular resistances decreased by 62 p. cent. Mean arterial pressure decreased by 53 p. cent; it reached 40 mm Hg. Fall in preload resulted in a decrease in pulmonary wedge pressure by 28 p. cent. This fall in preload produced a decrease in stroke index according to Frank-Starling's mechanisms. However tachycardia allowed a rise in cardiac index by 20 p. cent. Increase of pulmonary wedge pressure at 8-10 mm Hg by blood volume expansion maintains stroke index at control level. Under these conditions the elevation of cardiac index is due to tachycardia. Cardiac rhythm disorders (wandering pace-maker, nodal rhythm) are observed in 5 patients after having stopped nitroprusside.", "contents": "[Deep hypotension induced by sodium nitroprusside in neurosurgery. I.--Systemic hemodynamic effects (author's transl)]. The systemic hemodynamic effects of deep hypotension (MAP: 38 +/- 6 mm Hg) induced by sodium nitroprusside (S.N.) were studied in 20 patients who underwent surgery for cerebral aneurysm. The hemodynamic measurements were performed four times.: (1) during the preoperative period, (2) during stable anesthesia just before hypotension, (3) during stable hypotension, (4) 20 minutes after stopping nitroprusside. All patients were mechanically ventilated with a constant tidal volume and rate. Parameters for acid-base balance and Pa O2 were also recorded. Nitroprusside produces arterial and venous dilatation which results in a decrease of afterload and preload. The mean dosage of S. N. was 18 mcg/kg/mn. Systemic vascular resistances decreased by 62 p. cent. Mean arterial pressure decreased by 53 p. cent; it reached 40 mm Hg. Fall in preload resulted in a decrease in pulmonary wedge pressure by 28 p. cent. This fall in preload produced a decrease in stroke index according to Frank-Starling's mechanisms. However tachycardia allowed a rise in cardiac index by 20 p. cent. Increase of pulmonary wedge pressure at 8-10 mm Hg by blood volume expansion maintains stroke index at control level. Under these conditions the elevation of cardiac index is due to tachycardia. Cardiac rhythm disorders (wandering pace-maker, nodal rhythm) are observed in 5 patients after having stopped nitroprusside."}
{"id": "PMID:484891", "title": "Tolerance to nitrous oxide analgesia in rats and mice.", "content": "The purpose of these experiments was to characterize the nature of tolerance to the analgesic action of nitrous oxide. Analgesia was assessed in rats using a tail-flick latency test and in mice using an abdominal constriction test. Rats and mice were exposed to nitrous oxide, 75 per cent, the balance oxygen, continuously for 16--18 hours. On re-exposure to nitrous oxide 30 min later, these animals were found tolerant to nitrous oxide in that the analgesic response was decreased by at least 50 per cent. Animals tolerant to nitrous oxide were not tolerant to morphine. Morphine (0.25--1.5 mg/kg) produced equal degrees of analgesia in control and nitrous oxide-tolerant mice and rats. In contrast, rats made tolerant to morphine by repeated daily injections of as much as 400 mg/kg subcutaneously or by subcutaneous implantation of morphine pellets (75 mg, twice) showed a decreased analgesic response to nitrous oxide. Thus the cross-tolerance between nitrous oxide and morphine appears unique in that it is unidirectional.", "contents": "Tolerance to nitrous oxide analgesia in rats and mice. The purpose of these experiments was to characterize the nature of tolerance to the analgesic action of nitrous oxide. Analgesia was assessed in rats using a tail-flick latency test and in mice using an abdominal constriction test. Rats and mice were exposed to nitrous oxide, 75 per cent, the balance oxygen, continuously for 16--18 hours. On re-exposure to nitrous oxide 30 min later, these animals were found tolerant to nitrous oxide in that the analgesic response was decreased by at least 50 per cent. Animals tolerant to nitrous oxide were not tolerant to morphine. Morphine (0.25--1.5 mg/kg) produced equal degrees of analgesia in control and nitrous oxide-tolerant mice and rats. In contrast, rats made tolerant to morphine by repeated daily injections of as much as 400 mg/kg subcutaneously or by subcutaneous implantation of morphine pellets (75 mg, twice) showed a decreased analgesic response to nitrous oxide. Thus the cross-tolerance between nitrous oxide and morphine appears unique in that it is unidirectional."}
{"id": "PMID:484890", "title": "Comparison of a chloroprocaine--bupivacaine mixture with chloroprocaine and bupivacaine used individually for obstetric epidural analgesia.", "content": "Continuous lumbar epidural anesthesia was instituted in 49 healthy parturients who were randomly assigned to three treatment groups: 14 patients received chloroprocaine, 3 per cent; 19 received bupivacaine, 0.5 per cent, and 16 received a mixture containing chloroprocaine, 1.5 per cent, and bupivacaine, 0.375 per cent. Observations relating to the characteristics of the anesthetic block and to maternal and fetal well-being were made by a trained nurse observer. Times to onset of analgesia, times to maximum block, and adequacies of analgesia were similar in all groups. Bupivacaine lasted significantly longer than chloroprocaine or the mixture (68 versus 50 and 52 min, respectively, for the first injection), and caused the least motor block. No clinical superiority could be demonstrate for the mixture as compared with bupivacaine or chloroprocaine used individually. There was no sign of material or fetal toxicity with any of the three treatment regimens.", "contents": "Comparison of a chloroprocaine--bupivacaine mixture with chloroprocaine and bupivacaine used individually for obstetric epidural analgesia. Continuous lumbar epidural anesthesia was instituted in 49 healthy parturients who were randomly assigned to three treatment groups: 14 patients received chloroprocaine, 3 per cent; 19 received bupivacaine, 0.5 per cent, and 16 received a mixture containing chloroprocaine, 1.5 per cent, and bupivacaine, 0.375 per cent. Observations relating to the characteristics of the anesthetic block and to maternal and fetal well-being were made by a trained nurse observer. Times to onset of analgesia, times to maximum block, and adequacies of analgesia were similar in all groups. Bupivacaine lasted significantly longer than chloroprocaine or the mixture (68 versus 50 and 52 min, respectively, for the first injection), and caused the least motor block. No clinical superiority could be demonstrate for the mixture as compared with bupivacaine or chloroprocaine used individually. There was no sign of material or fetal toxicity with any of the three treatment regimens."}
{"id": "PMID:484893", "title": "An animal model of halothane hepatotoxicity: roles of enzyme induction and hypoxia.", "content": "Exposure of phenobarbital-pretreated male Sprague-Dawley rats to halothane, 1 per cent, for two hours under conditions of hypoxia (FIO2 0.14) resulted in extensive centrilobular necrosis within 24 hours. Accompanying the morphologic damage were an increase in serum glutamic pyruvic transminase (SGPT) and a decrease in hepatic microsomal cytochrmoe P-450. Glutathione levels in the liver were unchanged. Phenobarbital-pretreated rats anesthetized with halothane, 1 per cent, at FIO2 0.21 had only minor morphologic changes at 24 hours. Hepatic injury was not apparent in any non-phenobarbital-induced rat or in any induced animal exposed to ether at FIO2 0.10 or to halothane at FIO2 0.99. There was a 2.6-fold increase in the 24-hour urinary excretion of fluoride in those rats in which extensive centrilobular necrosis developed. The in-vivo covalent binding to lipids of 14C from 14C-halothane also was increased markedly when 14C-halothane was administered intraperitoneally to phenobarbital-induced rats maintained hypoxic (FIO2 0.14) for two hours. These results support the authors' hypothesis that halothane is metabolized to hepatotoxic intermediates by a reductive or non-oxygen-dependent cytochrome P-450-dependent pathway. This animal model of halothane-induced hepatotoxicity may be clinically relevant. A decrease in hepatic blood flow during halothane anesthesia may decrease the PO2 available to hepatocytes and thus direct the metabolism of halothane along its reductive, hepatotoxic pathway.", "contents": "An animal model of halothane hepatotoxicity: roles of enzyme induction and hypoxia. Exposure of phenobarbital-pretreated male Sprague-Dawley rats to halothane, 1 per cent, for two hours under conditions of hypoxia (FIO2 0.14) resulted in extensive centrilobular necrosis within 24 hours. Accompanying the morphologic damage were an increase in serum glutamic pyruvic transminase (SGPT) and a decrease in hepatic microsomal cytochrmoe P-450. Glutathione levels in the liver were unchanged. Phenobarbital-pretreated rats anesthetized with halothane, 1 per cent, at FIO2 0.21 had only minor morphologic changes at 24 hours. Hepatic injury was not apparent in any non-phenobarbital-induced rat or in any induced animal exposed to ether at FIO2 0.10 or to halothane at FIO2 0.99. There was a 2.6-fold increase in the 24-hour urinary excretion of fluoride in those rats in which extensive centrilobular necrosis developed. The in-vivo covalent binding to lipids of 14C from 14C-halothane also was increased markedly when 14C-halothane was administered intraperitoneally to phenobarbital-induced rats maintained hypoxic (FIO2 0.14) for two hours. These results support the authors' hypothesis that halothane is metabolized to hepatotoxic intermediates by a reductive or non-oxygen-dependent cytochrome P-450-dependent pathway. This animal model of halothane-induced hepatotoxicity may be clinically relevant. A decrease in hepatic blood flow during halothane anesthesia may decrease the PO2 available to hepatocytes and thus direct the metabolism of halothane along its reductive, hepatotoxic pathway."}
{"id": "PMID:484888", "title": "[Deep hypotension induced by sodium nitroprusside in neurosurgery. II.--Cerebral hemodynamic effects and metabolic rate of oxygen (author's transl)].", "content": "The cerebral hemodynamic effects of sodium nitroprusside (S. N.) have been the object of animal studies mainly. During the only human study performed, the drop in mean arterial pressure (MAP) is limited to 67 mm Hg. The study of the evolution of cerebral blood flow (CBF) in cases of more severe hypotension (MAP less than 45 mm Hg) seems of some interest. The study was composed of the measurement of the CBF using Xenon 133 and the calculation of cerebral vascular resistances (CVR) as well as cerebral metabolic rate of oxygen (CMRO2). Eleven patients with an average age of 37 years underwent surgery for cerebral aneurism 10 to 15 days after the inaugural hemorrhagic accident, under narconeuroleptanalgesia and stable ventilatory conditions. They are divided into 2 groups: Group I, 9 patients with normal levels of consciousness; Group II, 2 patients either agitated or obnubilated. The study is composed of 3 successive measures: (1) (T0) during stable anesthesia just prior to hypotension; (2) (T1) after 5 minutes of hypotension (MAP = 40 +/- 7 MM Hg); (3) (T2) 20 minutes after having stopped S. N. infusion. For the 9 patients in Group I, CBF remains unchanged at T1, the CVR decreases (p less than 0.001) and CMRO2 decreases (p less than 0.05). At T2 all of these parameters return to T0 values. The postoperative clinical evolution is favorable and uncomplicated. For the 2 patients in Group II the CBF, initially higher, falls from 20 to 30 p. cent at T1 with a drastic reduction in CMRO2. Despite the improvement of these parameters at T2, a prolonged postoperative coma is observed. This study suggests that CBF and CMRO2 are important parameters to monitor during controlled severe hypotension. Decrease in MAP must be less than 50 p. cent of control value and time-limited.", "contents": "[Deep hypotension induced by sodium nitroprusside in neurosurgery. II.--Cerebral hemodynamic effects and metabolic rate of oxygen (author's transl)]. The cerebral hemodynamic effects of sodium nitroprusside (S. N.) have been the object of animal studies mainly. During the only human study performed, the drop in mean arterial pressure (MAP) is limited to 67 mm Hg. The study of the evolution of cerebral blood flow (CBF) in cases of more severe hypotension (MAP less than 45 mm Hg) seems of some interest. The study was composed of the measurement of the CBF using Xenon 133 and the calculation of cerebral vascular resistances (CVR) as well as cerebral metabolic rate of oxygen (CMRO2). Eleven patients with an average age of 37 years underwent surgery for cerebral aneurism 10 to 15 days after the inaugural hemorrhagic accident, under narconeuroleptanalgesia and stable ventilatory conditions. They are divided into 2 groups: Group I, 9 patients with normal levels of consciousness; Group II, 2 patients either agitated or obnubilated. The study is composed of 3 successive measures: (1) (T0) during stable anesthesia just prior to hypotension; (2) (T1) after 5 minutes of hypotension (MAP = 40 +/- 7 MM Hg); (3) (T2) 20 minutes after having stopped S. N. infusion. For the 9 patients in Group I, CBF remains unchanged at T1, the CVR decreases (p less than 0.001) and CMRO2 decreases (p less than 0.05). At T2 all of these parameters return to T0 values. The postoperative clinical evolution is favorable and uncomplicated. For the 2 patients in Group II the CBF, initially higher, falls from 20 to 30 p. cent at T1 with a drastic reduction in CMRO2. Despite the improvement of these parameters at T2, a prolonged postoperative coma is observed. This study suggests that CBF and CMRO2 are important parameters to monitor during controlled severe hypotension. Decrease in MAP must be less than 50 p. cent of control value and time-limited."}
{"id": "PMID:484895", "title": "A simple implantable system for delivery of halothane.", "content": "A capsule for regulated halothane delivery after abdominal implantation in experimental animals is described. The capsule is constructed of readily available medical-grade polyethylene and Teflon tubing. At 37 C linear halothane release rates of 0.4 to 15 mg/hour may be obtained.", "contents": "A simple implantable system for delivery of halothane. A capsule for regulated halothane delivery after abdominal implantation in experimental animals is described. The capsule is constructed of readily available medical-grade polyethylene and Teflon tubing. At 37 C linear halothane release rates of 0.4 to 15 mg/hour may be obtained."}
{"id": "PMID:484912", "title": "A systems approach to the diagnosis of venous thrombosis and insufficiency.", "content": "This systems approach to the diagnosis of venous vascular obstruction and venous insufficiency relies on use of the segmental pneumoplethysmograph. The method is a useful diagnostic tool when evaluating these diseases. The drainage test indicates deep, large venous thrombosis. The dependency test indicates either thrombosis or venous insufficiency. And the tourniquet test differentiates the presence of deep from superficial vein insufficiency.", "contents": "A systems approach to the diagnosis of venous thrombosis and insufficiency. This systems approach to the diagnosis of venous vascular obstruction and venous insufficiency relies on use of the segmental pneumoplethysmograph. The method is a useful diagnostic tool when evaluating these diseases. The drainage test indicates deep, large venous thrombosis. The dependency test indicates either thrombosis or venous insufficiency. And the tourniquet test differentiates the presence of deep from superficial vein insufficiency."}
{"id": "PMID:484913", "title": "Subcutaneous ancrod therapy in peripheral arterial disease: improvement in blood viscosity and nutritional blood flow.", "content": "Nine patients with extensive peripheral arterial disease were treated with subcutaneous injections of ancrod (Arvin) for 10 to 21 days. Reduction in plasma fibrinogen was associated with a sustained reduction in plasma and blood viscosity, and a sustained increase in nutritional skin blood flow, measured by a Xenon-133 clearance technique (P less than 0.001). These findings may be relevant to the therapeutic effect of ancrod in ischemic rest pain.", "contents": "Subcutaneous ancrod therapy in peripheral arterial disease: improvement in blood viscosity and nutritional blood flow. Nine patients with extensive peripheral arterial disease were treated with subcutaneous injections of ancrod (Arvin) for 10 to 21 days. Reduction in plasma fibrinogen was associated with a sustained reduction in plasma and blood viscosity, and a sustained increase in nutritional skin blood flow, measured by a Xenon-133 clearance technique (P less than 0.001). These findings may be relevant to the therapeutic effect of ancrod in ischemic rest pain."}
{"id": "PMID:484914", "title": "Experience with a noninvasive evaluation for cerebral vascular disease.", "content": "This report describes a newly developed noninvasive method for extracranial cerebrovascular evaluation. Two hundred consecutive cases were studied at the Massachusetts General Hospital and matched with clinical and angiographic findings. There were three false negative and five false positive results. Two ulcerated plaques without stenosis were not detected. Management routes for different groups of patients with central nervous system symptoms are outlined. We emphasize the usefulness of monitoring postoperative ophthalmic artery pressure to aid in the early detection of carotid occlusion in patients developing neurologic symptoms following carotid endarterectomy.", "contents": "Experience with a noninvasive evaluation for cerebral vascular disease. This report describes a newly developed noninvasive method for extracranial cerebrovascular evaluation. Two hundred consecutive cases were studied at the Massachusetts General Hospital and matched with clinical and angiographic findings. There were three false negative and five false positive results. Two ulcerated plaques without stenosis were not detected. Management routes for different groups of patients with central nervous system symptoms are outlined. We emphasize the usefulness of monitoring postoperative ophthalmic artery pressure to aid in the early detection of carotid occlusion in patients developing neurologic symptoms following carotid endarterectomy."}
{"id": "PMID:484915", "title": "Plain roentgenographic estimation of systolic pulmonary artery pressure by the pulmonary arterial angle method.", "content": "Chest roentgenograms of 125 patients (115 with pure or predominant mitral stenosis) were studied by the pulmonary arterial angle method to estimate systolic pulmonary artery pressure without prior knowledge of catheterization data. First the angle between the line drawn along the right upper lobe artery and the tangent drawn along the point of junction of superior and lateral borders of the right pulmonary artery was determined. Next the angle between the right middle lobe artery and the descending pulmonary artery is determined. The difference between these angles equals systolic pulmonary artery pressure. Catheter and angle values were identical in 26 patients. There was a 1- to 5-mm difference in 70, a 6- to 10-mm difference in 18, an 11- to 15-mm difference in 8, and a difference greater than 15 mm in 3.", "contents": "Plain roentgenographic estimation of systolic pulmonary artery pressure by the pulmonary arterial angle method. Chest roentgenograms of 125 patients (115 with pure or predominant mitral stenosis) were studied by the pulmonary arterial angle method to estimate systolic pulmonary artery pressure without prior knowledge of catheterization data. First the angle between the line drawn along the right upper lobe artery and the tangent drawn along the point of junction of superior and lateral borders of the right pulmonary artery was determined. Next the angle between the right middle lobe artery and the descending pulmonary artery is determined. The difference between these angles equals systolic pulmonary artery pressure. Catheter and angle values were identical in 26 patients. There was a 1- to 5-mm difference in 70, a 6- to 10-mm difference in 18, an 11- to 15-mm difference in 8, and a difference greater than 15 mm in 3."}
{"id": "PMID:484917", "title": "\"Malignant\" brachial arteriovenous fistula in a newborn child: a case report.", "content": "From the time of birth, a patient a rapidly expanding pulsatile mass in the left upper extremity. The findings are those of multiple AV fistulae: thrill, continuous murmur, cutaneous angioma, and rapidly appearing trophic changes. Cyanosis and irreversible heart failure ensued, and the patient died after 20 hours of life. An aortogram revealed numerous AV fistulas within an angiomatous network vascularized by a huge subclavian artery and drained by a large subclavian vein.", "contents": "\"Malignant\" brachial arteriovenous fistula in a newborn child: a case report. From the time of birth, a patient a rapidly expanding pulsatile mass in the left upper extremity. The findings are those of multiple AV fistulae: thrill, continuous murmur, cutaneous angioma, and rapidly appearing trophic changes. Cyanosis and irreversible heart failure ensued, and the patient died after 20 hours of life. An aortogram revealed numerous AV fistulas within an angiomatous network vascularized by a huge subclavian artery and drained by a large subclavian vein."}
{"id": "PMID:484918", "title": "Heparin bleeding due to qualitative platelet dysfunction.", "content": "On two separate occasions, a 26-year-old white woman bled from arterial puncture wounds while receiving heparin for thromboembolic disease. Bleeding time was prolonged after heparin administration at the time that she was ill and bled, and when she was re-challenged 2 years later. Heparin may produce bleeding as a result of a qualitative platelet dysfunction.", "contents": "Heparin bleeding due to qualitative platelet dysfunction. On two separate occasions, a 26-year-old white woman bled from arterial puncture wounds while receiving heparin for thromboembolic disease. Bleeding time was prolonged after heparin administration at the time that she was ill and bled, and when she was re-challenged 2 years later. Heparin may produce bleeding as a result of a qualitative platelet dysfunction."}
{"id": "PMID:484928", "title": "The respiratory neuromuscular response to hypoxia, hypercapnia, and obstruction to airflow in asthma.", "content": "In chronic obstructive pulmonary disease (COPD), the neuromuscular response to an acute increase in airflow produced by external flow resistive loads (FRL) is impaired. The present study compared the response to FRL of 15 subjects with airway obstruction due to asthma and that of 15 normal subjects. FRL were applied during progressive hypercapnia and isocapnic hypoxia produced by rebreathing techniques to permit the response to be assessed at the same degree of CO2 or O2 drive. The neuromuscular response to FRL was assessed from the airway occlusion pressure developed 100 msec after the onset of inspiration (P100), as well as ventilation. During control rebreathing, ventilatory responses to hypercapnia (ratio of change in minute ventilation to change in PCO2, delta VE/delta PCO2) and hypoxia (ratio of change in VE to the change in percentage of O2 saturation, delta VE/deltaSO2) were the same in asthmatic and normal subjects despite differences in the mechanics of breathing. The P100 response to hypercapnia delta P100/delta PCO2) and hypoxia (delta P100/delta SO2) as well as absolute P100 at any given degree of O2 and CO2 drive was greater during control rebreathing in asthmatics than in normal subjects (P less than 0.05). FRL values of 9 and 18 cm H2O per L per sec applied during either hypercapnia or hypoxia increased the occlusion pressure to a greater extent in asthmatics than in normal subjects. Methacholine-induced bronchoconstriction was used to test the effect of acute airway obstruction on the response to FRL. Bronchoconstriction was associated with an increase in the P100 response to hypercapnia and to FRL, despite increases in lung volume and decreases in inspiratory muscle force. We conclude that: (1) asthmatics with airway dysfunction have an increased nonchemical drive to breathe mediated at least in part by sensory receptors in the airways; (2) asthmatics with airway obstruction respond supernormally to acute changes in resistance to airflow, unlike subjects with COPD. The failure of COPD subjects with prolonged airway obstruction to respond to FRL may be due to adaptation of the sensory mechanisms that respond to changes in airway resistance.", "contents": "The respiratory neuromuscular response to hypoxia, hypercapnia, and obstruction to airflow in asthma. In chronic obstructive pulmonary disease (COPD), the neuromuscular response to an acute increase in airflow produced by external flow resistive loads (FRL) is impaired. The present study compared the response to FRL of 15 subjects with airway obstruction due to asthma and that of 15 normal subjects. FRL were applied during progressive hypercapnia and isocapnic hypoxia produced by rebreathing techniques to permit the response to be assessed at the same degree of CO2 or O2 drive. The neuromuscular response to FRL was assessed from the airway occlusion pressure developed 100 msec after the onset of inspiration (P100), as well as ventilation. During control rebreathing, ventilatory responses to hypercapnia (ratio of change in minute ventilation to change in PCO2, delta VE/delta PCO2) and hypoxia (ratio of change in VE to the change in percentage of O2 saturation, delta VE/deltaSO2) were the same in asthmatic and normal subjects despite differences in the mechanics of breathing. The P100 response to hypercapnia delta P100/delta PCO2) and hypoxia (delta P100/delta SO2) as well as absolute P100 at any given degree of O2 and CO2 drive was greater during control rebreathing in asthmatics than in normal subjects (P less than 0.05). FRL values of 9 and 18 cm H2O per L per sec applied during either hypercapnia or hypoxia increased the occlusion pressure to a greater extent in asthmatics than in normal subjects. Methacholine-induced bronchoconstriction was used to test the effect of acute airway obstruction on the response to FRL. Bronchoconstriction was associated with an increase in the P100 response to hypercapnia and to FRL, despite increases in lung volume and decreases in inspiratory muscle force. We conclude that: (1) asthmatics with airway dysfunction have an increased nonchemical drive to breathe mediated at least in part by sensory receptors in the airways; (2) asthmatics with airway obstruction respond supernormally to acute changes in resistance to airflow, unlike subjects with COPD. The failure of COPD subjects with prolonged airway obstruction to respond to FRL may be due to adaptation of the sensory mechanisms that respond to changes in airway resistance."}
{"id": "PMID:484929", "title": "Significance of the quantification and demonstration of hyaluronic acid in tissue specimens for the diagnosis of pleural mesothelioma.", "content": "Hyaluronic acid in pleural tissues from patients with mesothelioma, carcinoma, and asbestosis of the lung was quantified by using specific glycosaminoglycan-degrading enzymes. In all cases of pleural mesothelioma, the quantity of hyaluronic acid in mg/g of dry tissue was at least 0.10 mg, whereas carcinomatous pleural tissue and pleura in asbestosis contained 0.02 to 0.03 mg/g of dry tissue.", "contents": "Significance of the quantification and demonstration of hyaluronic acid in tissue specimens for the diagnosis of pleural mesothelioma. Hyaluronic acid in pleural tissues from patients with mesothelioma, carcinoma, and asbestosis of the lung was quantified by using specific glycosaminoglycan-degrading enzymes. In all cases of pleural mesothelioma, the quantity of hyaluronic acid in mg/g of dry tissue was at least 0.10 mg, whereas carcinomatous pleural tissue and pleura in asbestosis contained 0.02 to 0.03 mg/g of dry tissue."}
{"id": "PMID:484930", "title": "Determination of pulmonary blood flow by the rebreathing technique in airflow obstruction.", "content": "To test the hypothesis that the effect of nonuniform gas distribution on the uptake of inhaled soluble gases in the lung is minimized by rebreathing, we measured pulmonary blood flow by the thermodilution technique, and pulmonary capillary blood flow, combined pulmonary tissue and capillary blood volume, diffusing capacity of the lung for CO, and alveolar volume by a rebreathing technique in conscious sheep before and immediately after inhalation of a histamine aerosol (4% solution). We also measured pulmonary resistance, distribution of ventilation by multiple-breath N2 washout, and arterial blood gases to monitor airway responses. Histamine inhalation produced a mean pulmonary resistance increase to 621% of baseline accompanied by uneven distribution of ventilation and a decrease in arterial Po2. Despite these alterations in airway function, there were no concomitant changes in pulmonary capillary blood flow, combined pulmonary tissue and capillary blood volume, diffusing capacity of the lung for CO, alveolar volume, or the ratio of diffusing capacity for CO to alveolar volume. Mean pulmonary capillary blood flow and pulmonary blood flow did not differ from each other before and after histamine inhalation. We conclude that the rebreathing technique for noninvasive measurement of pulmonary hemodynamics is satisfactory in the presence of airflow obstruction.", "contents": "Determination of pulmonary blood flow by the rebreathing technique in airflow obstruction. To test the hypothesis that the effect of nonuniform gas distribution on the uptake of inhaled soluble gases in the lung is minimized by rebreathing, we measured pulmonary blood flow by the thermodilution technique, and pulmonary capillary blood flow, combined pulmonary tissue and capillary blood volume, diffusing capacity of the lung for CO, and alveolar volume by a rebreathing technique in conscious sheep before and immediately after inhalation of a histamine aerosol (4% solution). We also measured pulmonary resistance, distribution of ventilation by multiple-breath N2 washout, and arterial blood gases to monitor airway responses. Histamine inhalation produced a mean pulmonary resistance increase to 621% of baseline accompanied by uneven distribution of ventilation and a decrease in arterial Po2. Despite these alterations in airway function, there were no concomitant changes in pulmonary capillary blood flow, combined pulmonary tissue and capillary blood volume, diffusing capacity of the lung for CO, alveolar volume, or the ratio of diffusing capacity for CO to alveolar volume. Mean pulmonary capillary blood flow and pulmonary blood flow did not differ from each other before and after histamine inhalation. We conclude that the rebreathing technique for noninvasive measurement of pulmonary hemodynamics is satisfactory in the presence of airflow obstruction."}
{"id": "PMID:484931", "title": "The effect of hyperoxia on migration of alveolar macrophages in vitro.", "content": "There is in vitro evidence to support the notion that directed migration (chemotaxis) is involved in the recruitment of alveolar macrophages in vivo. Because O2 is widely used in the treatment of pulmonary diseases, we examined the effect of hyperoxia on migration of guinea pig alveolar macrophages in vitro. Migration was measured in blind-well chambers incubated in either room air or hyperoxia. N-formyl-methionyl-phenylalanine was used to stimulate random migration and to produce directed migration. Migration was quantified by counting the number of mononuclear cells per oil immersion field that had migrated completely through a polycarbonate filter with 5-micrometer pores. The average PO2 in the cell suspensions incubated in room air was 100 mm Hg. In the hyperoxic environments, the average PO2 at 1 h was 260 mm Hg, whereas at 2 and 3 h, it was 410 and 425 mm Hg, respectively. In 6 separate experiments, there was no significant difference between the mean response to N-formyl-methionyl phenylalanine in hyperoxia and in room air after 1 h of incubation. After 2 and 3h of incubation, however, the response in hyperoxia was significantly (P less than 0.002) lower than that in room air. The decreased response in hyperoxia did not appear to result from loss of viability of responding cells, diminished adherence of cells to the filters, loss of activity of N-formyl-methionyl phenylalanine exposed to high PO2, or failure of the cells to exhibit directed migration. Instead, it appeared that hyperoxia decreased the response of alveolar macrophages primarily by impairing random migration.", "contents": "The effect of hyperoxia on migration of alveolar macrophages in vitro. There is in vitro evidence to support the notion that directed migration (chemotaxis) is involved in the recruitment of alveolar macrophages in vivo. Because O2 is widely used in the treatment of pulmonary diseases, we examined the effect of hyperoxia on migration of guinea pig alveolar macrophages in vitro. Migration was measured in blind-well chambers incubated in either room air or hyperoxia. N-formyl-methionyl-phenylalanine was used to stimulate random migration and to produce directed migration. Migration was quantified by counting the number of mononuclear cells per oil immersion field that had migrated completely through a polycarbonate filter with 5-micrometer pores. The average PO2 in the cell suspensions incubated in room air was 100 mm Hg. In the hyperoxic environments, the average PO2 at 1 h was 260 mm Hg, whereas at 2 and 3 h, it was 410 and 425 mm Hg, respectively. In 6 separate experiments, there was no significant difference between the mean response to N-formyl-methionyl phenylalanine in hyperoxia and in room air after 1 h of incubation. After 2 and 3h of incubation, however, the response in hyperoxia was significantly (P less than 0.002) lower than that in room air. The decreased response in hyperoxia did not appear to result from loss of viability of responding cells, diminished adherence of cells to the filters, loss of activity of N-formyl-methionyl phenylalanine exposed to high PO2, or failure of the cells to exhibit directed migration. Instead, it appeared that hyperoxia decreased the response of alveolar macrophages primarily by impairing random migration."}
{"id": "PMID:484932", "title": "Effect of terbutaline on movement of Cl- and Na+ across the trachea of the dog in vitro.", "content": "The epithelium of canine trachea maintains a transepithelial potential difference (lumen negative relative to submucosa) by active transport of Cl- toward the lumen and of Na+ away from the lumen. This study examined the effects of terbutaline (a beta-adrenergic agonist) on short-circuit current, potential difference, electrical conductance, and fluxes of 36Cl and 24Na measured across pieces of canine trachea mounted in Ussing-type chambers. In short-circuited tissues, 10(-5) M terbutaline increased unidirectional Cl- flux toward the lumen from 4.78 +/- 1.12 (mean +/- SEM) to 6.28 +/- 1.06 muEq/cm2.h. The other measured fluxes did not change significantly. Increases in unidirectional Cl- flux toward the lumen were related to terbutaline concentration (range, 10(-7) to 10(-5) M); 10(-6) M propranolol prevented or decreased these effects. These studies suggest that terbutaline acts via beta-adrenergic receptors to increase active transport of Cl- toward the lumen.", "contents": "Effect of terbutaline on movement of Cl- and Na+ across the trachea of the dog in vitro. The epithelium of canine trachea maintains a transepithelial potential difference (lumen negative relative to submucosa) by active transport of Cl- toward the lumen and of Na+ away from the lumen. This study examined the effects of terbutaline (a beta-adrenergic agonist) on short-circuit current, potential difference, electrical conductance, and fluxes of 36Cl and 24Na measured across pieces of canine trachea mounted in Ussing-type chambers. In short-circuited tissues, 10(-5) M terbutaline increased unidirectional Cl- flux toward the lumen from 4.78 +/- 1.12 (mean +/- SEM) to 6.28 +/- 1.06 muEq/cm2.h. The other measured fluxes did not change significantly. Increases in unidirectional Cl- flux toward the lumen were related to terbutaline concentration (range, 10(-7) to 10(-5) M); 10(-6) M propranolol prevented or decreased these effects. These studies suggest that terbutaline acts via beta-adrenergic receptors to increase active transport of Cl- toward the lumen."}
{"id": "PMID:484933", "title": "Effect of local radioactivity on trachael mucous velocity of sheep.", "content": "The effects of local isotope radiation and x-ray radiation on mucociliary transport of sheep were investigated. X-ray radiation from fluoroscopy equivalent to that used to determine tracheal mucous velocity in intact animals (approximately 3 min) did not alter transport of teflon discs in an isolated sheep trachael preparation. In conscious sheep, 2 ml of a solution containing 8 mCi of macroaggregates of albumin labeled with technetium-99m (99mTc) and 99mTc-pyrophosphate were instilled under direct vision into the trachea via a fiberoptic bronchoscope, providing an actual deposition on the tracheal epithelium of 1.8 to 2 mCi. Physiologic saline and unlabeled macroaggregates of albumin were instilled as control solutions. Tracheal mucous velocity measured by movement of teflon discs increased by 93.2 per cent with 99mTc-labeled macroaggregated albumin and 86.7 per cent with 99mTc-pyrophosphate (P less than 0.01), but no significant increase occurred after saline or unlabeled macroaggregates of albumin. Discrepancies in the values of trachael mucous velocity determined using inert particles and radioactive tracer solution or aerosols might be related in part to the local effects of the tracer radiation.", "contents": "Effect of local radioactivity on trachael mucous velocity of sheep. The effects of local isotope radiation and x-ray radiation on mucociliary transport of sheep were investigated. X-ray radiation from fluoroscopy equivalent to that used to determine tracheal mucous velocity in intact animals (approximately 3 min) did not alter transport of teflon discs in an isolated sheep trachael preparation. In conscious sheep, 2 ml of a solution containing 8 mCi of macroaggregates of albumin labeled with technetium-99m (99mTc) and 99mTc-pyrophosphate were instilled under direct vision into the trachea via a fiberoptic bronchoscope, providing an actual deposition on the tracheal epithelium of 1.8 to 2 mCi. Physiologic saline and unlabeled macroaggregates of albumin were instilled as control solutions. Tracheal mucous velocity measured by movement of teflon discs increased by 93.2 per cent with 99mTc-labeled macroaggregated albumin and 86.7 per cent with 99mTc-pyrophosphate (P less than 0.01), but no significant increase occurred after saline or unlabeled macroaggregates of albumin. Discrepancies in the values of trachael mucous velocity determined using inert particles and radioactive tracer solution or aerosols might be related in part to the local effects of the tracer radiation."}
{"id": "PMID:484934", "title": "Anchoring fibrils in the normal canine respiratory system.", "content": "Electron microscopic study of the normal canine respiratory tree disclosed the presence of anchoring fibrils, a distinct class of fibrils of extracellular connective tissue, in association with the following types of cells: (1) basal cells and special type cells of trachea and bronchi; (2) ciliated cells and basal cells of bronchioles; (3) ductal cells, secretory cells, and myoepithelial cells of tracheobronchial glands. Anchoring fibrils in the normal respiratory system measured up to 6,000 A in length and from 170 to 400 A in thickness, and had a banding pattern that differed from that of collagen fibrils and connective tissue microfibrils. They formed arcs, the ends of which inserted into the basal lamina underlying the basal portions of the cells, often in the vicinity of hemidesmosomes. Anchoring fibrils decreased in number and size in the more distal portions of the respiratory tree, and were not found in alveolar septums. Anchoring fibrils in lung appeared similar to those described in other organs, but were often small and inconspicuous. The function of these structures is to reinforce the attachment of the epithelial basal lamina to the underlying connective tissues.", "contents": "Anchoring fibrils in the normal canine respiratory system. Electron microscopic study of the normal canine respiratory tree disclosed the presence of anchoring fibrils, a distinct class of fibrils of extracellular connective tissue, in association with the following types of cells: (1) basal cells and special type cells of trachea and bronchi; (2) ciliated cells and basal cells of bronchioles; (3) ductal cells, secretory cells, and myoepithelial cells of tracheobronchial glands. Anchoring fibrils in the normal respiratory system measured up to 6,000 A in length and from 170 to 400 A in thickness, and had a banding pattern that differed from that of collagen fibrils and connective tissue microfibrils. They formed arcs, the ends of which inserted into the basal lamina underlying the basal portions of the cells, often in the vicinity of hemidesmosomes. Anchoring fibrils decreased in number and size in the more distal portions of the respiratory tree, and were not found in alveolar septums. Anchoring fibrils in lung appeared similar to those described in other organs, but were often small and inconspicuous. The function of these structures is to reinforce the attachment of the epithelial basal lamina to the underlying connective tissues."}
{"id": "PMID:484935", "title": "Defective oxidative metabolic responses in vitro of alveolar macrophages in chronic granulomatous disease.", "content": "After stimulation with bacteria, alveolar macrophages (AM) from uninfected normal subjects or persons with pneumonia approximately doubled their rates of O2 consumption, superoxide anion generation, and glucose (1(-14)C) oxidation. In contrast, bacteria-stimulated AM from a patient with chronic granulomatous disease (CGD) failed to consume more O2, make superoxide anion, or oxidize glucose. In addition, AM from the patient with CGD did not respond to stimulation by a chemical agent, phorbol myristate acetate, which increased the metabolic activities of AM from control subjects. The appearance, esterase and Gomori acid phosphatase staining, phagocytic ability, unstimulated O2 consumption, and response to methylene blue of AM from the CGD patient were normal. The results extend the biochemical defect in patients with CGD beyond abnormalities in their circulating neutrophils and monocytes, to their tissue-associated lung macrophages. The results also indicate that AM from patients with CGD may have an additional abnormality in metabolism, which is a lack of enhanced mitochondrial respiration during phagocytosis. The studies also document the selective action of phorbol myristate acetate, which stimulated the metabolic activities of normal AM, but not of those from the patient with CGD.", "contents": "Defective oxidative metabolic responses in vitro of alveolar macrophages in chronic granulomatous disease. After stimulation with bacteria, alveolar macrophages (AM) from uninfected normal subjects or persons with pneumonia approximately doubled their rates of O2 consumption, superoxide anion generation, and glucose (1(-14)C) oxidation. In contrast, bacteria-stimulated AM from a patient with chronic granulomatous disease (CGD) failed to consume more O2, make superoxide anion, or oxidize glucose. In addition, AM from the patient with CGD did not respond to stimulation by a chemical agent, phorbol myristate acetate, which increased the metabolic activities of AM from control subjects. The appearance, esterase and Gomori acid phosphatase staining, phagocytic ability, unstimulated O2 consumption, and response to methylene blue of AM from the CGD patient were normal. The results extend the biochemical defect in patients with CGD beyond abnormalities in their circulating neutrophils and monocytes, to their tissue-associated lung macrophages. The results also indicate that AM from patients with CGD may have an additional abnormality in metabolism, which is a lack of enhanced mitochondrial respiration during phagocytosis. The studies also document the selective action of phorbol myristate acetate, which stimulated the metabolic activities of normal AM, but not of those from the patient with CGD."}
{"id": "PMID:484936", "title": "Various antigenic reactivities in delayed hypersensitivity among crystalline proteins from Mycobacterium phlei.", "content": "Comparisons were made of the delayed-type skin reactivity of 6 crystalline proteins purified from the cell extract of Mycobacterium phlei in guinea pigs sensitized with whole cells of the heat-killed bacillus. These highly purified proteins elicited varying degrees of cutaneous reaction. The most active protein had almost the same reactivity as purified protein derivative prepared from the culture filtrate of Mycobacterium phlei. On the other hand, the weakest protein did not elicit a marked cutaneous reaction even after injection of 3,000 times the amount of protein of the most potent one. The other 4 proteins showed moderate reactivities. The difference in antigenic potency between proteins is probably due not to the amounts of the proteins contained in the cells used for sensitization, but to their structure.", "contents": "Various antigenic reactivities in delayed hypersensitivity among crystalline proteins from Mycobacterium phlei. Comparisons were made of the delayed-type skin reactivity of 6 crystalline proteins purified from the cell extract of Mycobacterium phlei in guinea pigs sensitized with whole cells of the heat-killed bacillus. These highly purified proteins elicited varying degrees of cutaneous reaction. The most active protein had almost the same reactivity as purified protein derivative prepared from the culture filtrate of Mycobacterium phlei. On the other hand, the weakest protein did not elicit a marked cutaneous reaction even after injection of 3,000 times the amount of protein of the most potent one. The other 4 proteins showed moderate reactivities. The difference in antigenic potency between proteins is probably due not to the amounts of the proteins contained in the cells used for sensitization, but to their structure."}
{"id": "PMID:484937", "title": "The effect of helium on nasal resistance and nasal flows.", "content": "Nasal inspiratory resistance and maximal inspiratory nasal flow were measured in 10 normal subjects while they breathed air and while they breathed a mixture of 80% helium and 20% O2. After the less dense helium-O2 mixture, there was a nonsignificant increase in K1 (15 +/- 93%), a 56 +/- 20% decrease in K2 (P less than 0.001), and a 48 +/- 20% increase in maximal inspiratory nasal flow (P less than 0.001). This is consistent with the accepted concept that K1 represents resistance to laminar flow and K2, resistance to nonlaminar flow (turbulent flow and/or flow due to convective acceleration), and that nonlaminar air flow predominates in the nose.", "contents": "The effect of helium on nasal resistance and nasal flows. Nasal inspiratory resistance and maximal inspiratory nasal flow were measured in 10 normal subjects while they breathed air and while they breathed a mixture of 80% helium and 20% O2. After the less dense helium-O2 mixture, there was a nonsignificant increase in K1 (15 +/- 93%), a 56 +/- 20% decrease in K2 (P less than 0.001), and a 48 +/- 20% increase in maximal inspiratory nasal flow (P less than 0.001). This is consistent with the accepted concept that K1 represents resistance to laminar flow and K2, resistance to nonlaminar flow (turbulent flow and/or flow due to convective acceleration), and that nonlaminar air flow predominates in the nose."}
{"id": "PMID:484940", "title": "[Establishment of circadian rhythm of cortisol in newborn and older children (author's transl)].", "content": "In 30 children, 19 males and 11 females, aged 5 days to 36 months admitted for reasons other than neurological, endocrine, hepatic or renal diseases, the plasma cortisol levels at 9.00 and 20.00 hr. was studied to ascertain the time of appearance of a pattern of circadian rhythm comparable to adults. Children were arbitrarily divided into three groups according to their age: group I (n = 9) 5 days to 6 months; group II (n = 13) 7 to 17 months and group III (n = 8) 24 to 36 months old. The plasma corticol levels at 9.00 and 20.00 hr. were respectively for each group (X +/- SD): 12.7 +/- 8.0; 10.9 +/- 8.62; 17.7 +/- 6.65; 11.9 +/- 7.5 and 21.6 +/- 4.67; 9.7 +/- 3.8 microgram/100 ml. Evidence for circadian rhythm was statistically demonstrated only in group III and from these individual results the criteria to be satisfied as evidence of rhythm are: the cortisol value at 20.00 hr. must be less than or equal to 15.0 microgram/100 ml. with a decrease greater than or equal to 30% compared to basal (9.00 hr.) value. Only 7 out of 13 children in group II and 4 out of the 9 children in group I conform such criteria. These results show that the circadian rhythm of cortisol might be used as a valuable test to study adrenal function in children from the age of two.", "contents": "[Establishment of circadian rhythm of cortisol in newborn and older children (author's transl)]. In 30 children, 19 males and 11 females, aged 5 days to 36 months admitted for reasons other than neurological, endocrine, hepatic or renal diseases, the plasma cortisol levels at 9.00 and 20.00 hr. was studied to ascertain the time of appearance of a pattern of circadian rhythm comparable to adults. Children were arbitrarily divided into three groups according to their age: group I (n = 9) 5 days to 6 months; group II (n = 13) 7 to 17 months and group III (n = 8) 24 to 36 months old. The plasma corticol levels at 9.00 and 20.00 hr. were respectively for each group (X +/- SD): 12.7 +/- 8.0; 10.9 +/- 8.62; 17.7 +/- 6.65; 11.9 +/- 7.5 and 21.6 +/- 4.67; 9.7 +/- 3.8 microgram/100 ml. Evidence for circadian rhythm was statistically demonstrated only in group III and from these individual results the criteria to be satisfied as evidence of rhythm are: the cortisol value at 20.00 hr. must be less than or equal to 15.0 microgram/100 ml. with a decrease greater than or equal to 30% compared to basal (9.00 hr.) value. Only 7 out of 13 children in group II and 4 out of the 9 children in group I conform such criteria. These results show that the circadian rhythm of cortisol might be used as a valuable test to study adrenal function in children from the age of two."}
{"id": "PMID:484941", "title": "[Newborn of diabetic mother. Glucose in amniotic fluid and clinical conditions (author's transl)].", "content": "Clinical and analytical data of sixteen newborns of insulin dependant mothers are studied. Relation between these data and newborn conditions as Apgar score and hypoglicemia are considered.", "contents": "[Newborn of diabetic mother. Glucose in amniotic fluid and clinical conditions (author's transl)]. Clinical and analytical data of sixteen newborns of insulin dependant mothers are studied. Relation between these data and newborn conditions as Apgar score and hypoglicemia are considered."}
{"id": "PMID:484942", "title": "[Cystic adenomatoid malformation of the lung (author's transl)].", "content": "Four cases of congenital cystic adenomatoid malformation are presented. The age of the patients ranged from 1 day to 7 years. All cases were treated surgically and 3 of them survived. The only death in the group corresponded to the neonatal case. The clinical, radiological and patological features of congenital cystic adenomatoid malformation are also reviewed. Occasionally the clinical manifestations will be hardly noticeable or non existent and the malformation may be diagnosed beyond the neonatal period in the course of a radiological examination performed as routine or because of repeated respiratory infections. Surgery is the treatment of choice. In the group of neonates where the cystic adenomatoid malformation doesn't cause respiratory difficulty the authors feel that it might be advisable to postpone surgery until a later date.", "contents": "[Cystic adenomatoid malformation of the lung (author's transl)]. Four cases of congenital cystic adenomatoid malformation are presented. The age of the patients ranged from 1 day to 7 years. All cases were treated surgically and 3 of them survived. The only death in the group corresponded to the neonatal case. The clinical, radiological and patological features of congenital cystic adenomatoid malformation are also reviewed. Occasionally the clinical manifestations will be hardly noticeable or non existent and the malformation may be diagnosed beyond the neonatal period in the course of a radiological examination performed as routine or because of repeated respiratory infections. Surgery is the treatment of choice. In the group of neonates where the cystic adenomatoid malformation doesn't cause respiratory difficulty the authors feel that it might be advisable to postpone surgery until a later date."}
{"id": "PMID:484943", "title": "[Familial vesicoureteral reflux (author's transl)].", "content": "Sixteen members of three families, eight of which had vesicoureteral reflux, are studied. Authors report a high incidence in females (7 cases) and atrophic chronic pyelonephritis (75% of the serie). Only one girl presented disminution of renal function. The types of inheritance was dominant autosomal in the A family and autosomal recessive in the B and C families. Finally the norms of investigation of the familial vesicoureteral reflux are appointed.", "contents": "[Familial vesicoureteral reflux (author's transl)]. Sixteen members of three families, eight of which had vesicoureteral reflux, are studied. Authors report a high incidence in females (7 cases) and atrophic chronic pyelonephritis (75% of the serie). Only one girl presented disminution of renal function. The types of inheritance was dominant autosomal in the A family and autosomal recessive in the B and C families. Finally the norms of investigation of the familial vesicoureteral reflux are appointed."}
{"id": "PMID:484944", "title": "[Diagnostic and etiologic problems of hydranencephaly (author's transl)].", "content": "A report of hydranencephaly is made, featuring normal cephalic perimeter and where diagnosis was a pathological finding. There were no clinical suspiscions and fetal distress along with post-partum anoxia were thought to be the chief causes of the disease. A revision of clinical, diagnostic and etiological factors of this particular entity is made. It is of concern to aim this report in the direction of considering phosphorous-organic products to have a teratogenic action and therefore to be an interesting etiological aspect in the production of hydranencephaly.", "contents": "[Diagnostic and etiologic problems of hydranencephaly (author's transl)]. A report of hydranencephaly is made, featuring normal cephalic perimeter and where diagnosis was a pathological finding. There were no clinical suspiscions and fetal distress along with post-partum anoxia were thought to be the chief causes of the disease. A revision of clinical, diagnostic and etiological factors of this particular entity is made. It is of concern to aim this report in the direction of considering phosphorous-organic products to have a teratogenic action and therefore to be an interesting etiological aspect in the production of hydranencephaly."}
{"id": "PMID:484945", "title": "[Two cases of secondary amyloidosis (author's transl)].", "content": "Two cases of secondary amyloidosis are presented. Both cases presented as nephrotic syndrome and were confirmed by biopsy dying two years later of the appearance of proteinuria. Incidence and characteristics of amyloidosis in infancy is commented.", "contents": "[Two cases of secondary amyloidosis (author's transl)]. Two cases of secondary amyloidosis are presented. Both cases presented as nephrotic syndrome and were confirmed by biopsy dying two years later of the appearance of proteinuria. Incidence and characteristics of amyloidosis in infancy is commented."}
{"id": "PMID:484946", "title": "[Myocardial fibrosis in Shwachman's syndrome (author's transl)].", "content": "A case of a child affected by Shwachman's syndrome with a chronic diarrhea, cyclic neutropenia and bone defects is described. The child died at the age of eight months from an acute cardiac failure which showed analytical alterations compatible to an acute myocardial infarction. Pathologic study showed a pancreatic lipomatous hypoplasia, myocardial fibrosis and chondrometaphyseal dysplasia. Main hypothesis that relate pancreatic pathology and development of myocardial fibrosis are discussed, although there is a lack of definitive evidence for confirming such relation.", "contents": "[Myocardial fibrosis in Shwachman's syndrome (author's transl)]. A case of a child affected by Shwachman's syndrome with a chronic diarrhea, cyclic neutropenia and bone defects is described. The child died at the age of eight months from an acute cardiac failure which showed analytical alterations compatible to an acute myocardial infarction. Pathologic study showed a pancreatic lipomatous hypoplasia, myocardial fibrosis and chondrometaphyseal dysplasia. Main hypothesis that relate pancreatic pathology and development of myocardial fibrosis are discussed, although there is a lack of definitive evidence for confirming such relation."}
{"id": "PMID:484947", "title": "[Stewart and Bergstrom Syndrome. Contribution of a new observation (author's transl)].", "content": "Authors report a new observation to the Stewart and Bergstrom syndrome, which to their knowledge, can be the second case in the medical literature.", "contents": "[Stewart and Bergstrom Syndrome. Contribution of a new observation (author's transl)]. Authors report a new observation to the Stewart and Bergstrom syndrome, which to their knowledge, can be the second case in the medical literature."}
{"id": "PMID:484950", "title": "Arteriohepatic dysplasia: a benign syndrome of intrahepatic cholestasis with multiple organ involvement.", "content": "Arteriohepatic dysplasia (Alagille's syndrome) is presumed to be one of the familial intrahepatic cholestatic syndromes, all of which present with neonatal jaundice or failure to thrive, or both. We report the findings in five patients with this syndrome, four of whom have been followed into adulthood. In addition to hepatic dysfunction, patients had abnormalities of the cardiovascular system, eyes, bones, central nervous system, kidney, endocrine system, and habitus. Analysis of these cases allows a more complete characterization of this syndrome and shows that the cholestasis improves, although the abnormalities of the hands and face become more pronounced, with age. Patients with arteriohepatic dysplasia display the variability in expression seen in many autosomal-dominant conditions. New findings in the eye and spine provide markers specific for this syndrome and serve to differentiate it from other forms of cholestatic liver disease.", "contents": "Arteriohepatic dysplasia: a benign syndrome of intrahepatic cholestasis with multiple organ involvement. Arteriohepatic dysplasia (Alagille's syndrome) is presumed to be one of the familial intrahepatic cholestatic syndromes, all of which present with neonatal jaundice or failure to thrive, or both. We report the findings in five patients with this syndrome, four of whom have been followed into adulthood. In addition to hepatic dysfunction, patients had abnormalities of the cardiovascular system, eyes, bones, central nervous system, kidney, endocrine system, and habitus. Analysis of these cases allows a more complete characterization of this syndrome and shows that the cholestasis improves, although the abnormalities of the hands and face become more pronounced, with age. Patients with arteriohepatic dysplasia display the variability in expression seen in many autosomal-dominant conditions. New findings in the eye and spine provide markers specific for this syndrome and serve to differentiate it from other forms of cholestatic liver disease."}
{"id": "PMID:484951", "title": "Sj\u00f6gren's syndrome: a defect in reticuloendothelial system Fc-receptor-specific clearance.", "content": "To determine the functional status of reticuloendothelial system Fc receptors in patients with Sj\u00f6gren's syndrome, we studied the rate of clearance from the circulation of 51Cr-labeled IgG-sensitized autologous erythrocytes in 19 patients. Fc-receptor-mediated clearance was abnormal in 12 of the 19 patients, with half-lives ranging from 80 to 356 min. There was a significant correlation between clearance rates and clinical manifestations of disease. Clearance rates tended to be normal in patients with disease limited to exocrine glands and abnormal in patients with widespread disease. In contrast, there were no correlations between the rate of clearance of IgG-sensitized erythrocytes and serum immune complex levels, serum complement component levels, or rheumatoid factor titers. The striking correlation between clearance rates and disease manifestations suggests that decreased clearance of immune complexes by defective reticuloendothelial system Fc receptors may contribute to disease pathogenesis.", "contents": "Sj\u00f6gren's syndrome: a defect in reticuloendothelial system Fc-receptor-specific clearance. To determine the functional status of reticuloendothelial system Fc receptors in patients with Sj\u00f6gren's syndrome, we studied the rate of clearance from the circulation of 51Cr-labeled IgG-sensitized autologous erythrocytes in 19 patients. Fc-receptor-mediated clearance was abnormal in 12 of the 19 patients, with half-lives ranging from 80 to 356 min. There was a significant correlation between clearance rates and clinical manifestations of disease. Clearance rates tended to be normal in patients with disease limited to exocrine glands and abnormal in patients with widespread disease. In contrast, there were no correlations between the rate of clearance of IgG-sensitized erythrocytes and serum immune complex levels, serum complement component levels, or rheumatoid factor titers. The striking correlation between clearance rates and disease manifestations suggests that decreased clearance of immune complexes by defective reticuloendothelial system Fc receptors may contribute to disease pathogenesis."}
{"id": "PMID:484952", "title": "Resin hemoperfusion for treatment of ethchlorvynol overdose.", "content": "Hemoperfusion has been increasingly used to treat drug intoxication. Although efficient extraction rates have been reported with hemoperfusion devices, simultaneous measurements of drug adsorption on the columns and endogenous clearance rates have not been made. We studied three patients who ingested 12 to 22 g of ethchlorvynol (Placidyl). In each  patient saline diuresis was induced, and Amberlite XAD-4 resin hemoperfusion was done. Resin extraction of ethchlorvynol was calculated hourly, and the recovery of drugs from each column was measured. Renal clearance of ethchlorvynol was simultaneously measured. The resin removed 100% of perfused ethchlorvynol measured by gas chromatography. Ethchlorvynol recovery from the columns agreed well with calculated extraction. Renal clearance was 1 to 6 mL/min, and estimated metabolic clearance was 16% to 31% of resin extraction rate. Complications included anemia, thrombocytopenia, hypocalcemia, and pancreatitis. Resin hemoperfusion substantially supplements endogenous clearance of ethchlorvynol and is a valuable therapy in ethchlorvynol intoxication.", "contents": "Resin hemoperfusion for treatment of ethchlorvynol overdose. Hemoperfusion has been increasingly used to treat drug intoxication. Although efficient extraction rates have been reported with hemoperfusion devices, simultaneous measurements of drug adsorption on the columns and endogenous clearance rates have not been made. We studied three patients who ingested 12 to 22 g of ethchlorvynol (Placidyl). In each  patient saline diuresis was induced, and Amberlite XAD-4 resin hemoperfusion was done. Resin extraction of ethchlorvynol was calculated hourly, and the recovery of drugs from each column was measured. Renal clearance of ethchlorvynol was simultaneously measured. The resin removed 100% of perfused ethchlorvynol measured by gas chromatography. Ethchlorvynol recovery from the columns agreed well with calculated extraction. Renal clearance was 1 to 6 mL/min, and estimated metabolic clearance was 16% to 31% of resin extraction rate. Complications included anemia, thrombocytopenia, hypocalcemia, and pancreatitis. Resin hemoperfusion substantially supplements endogenous clearance of ethchlorvynol and is a valuable therapy in ethchlorvynol intoxication."}
{"id": "PMID:484953", "title": "Streptococcus bovis septicemia and carcinoma of the colon.", "content": "We prospectively studied patients with Streptococcus bovis septicemia for the presence of gastrointestinal lesions. This study was prompted by our reported findings of the association of fecal carriage of S. bovis with carcinoma of the colon. We studied 29 patients with 30 episodes of S. bovis septicemia. Fifteen completed gastrointestinal evaluations that included colonscopy, surgery, or autopsy. Eight of these had carcinoma of the colon, three had adenomatous polyps of the colon without carcinoma, and two had carcinoma of the esophagus. The 14 patients who did not have complete evaluations included one each with carcinoma of the stomach, gastric lymphoma, and adenomatous polyp of the colon and three with colonic masses not further delineated. Nineteen patients had no gastrointestinal signs or symptoms or stools positive for occult blood at admission. The results of our study suggest that all patients with S. bovis septicemia need aggressive evaluation of the gastrointestinal tract, especially the colon.", "contents": "Streptococcus bovis septicemia and carcinoma of the colon. We prospectively studied patients with Streptococcus bovis septicemia for the presence of gastrointestinal lesions. This study was prompted by our reported findings of the association of fecal carriage of S. bovis with carcinoma of the colon. We studied 29 patients with 30 episodes of S. bovis septicemia. Fifteen completed gastrointestinal evaluations that included colonscopy, surgery, or autopsy. Eight of these had carcinoma of the colon, three had adenomatous polyps of the colon without carcinoma, and two had carcinoma of the esophagus. The 14 patients who did not have complete evaluations included one each with carcinoma of the stomach, gastric lymphoma, and adenomatous polyp of the colon and three with colonic masses not further delineated. Nineteen patients had no gastrointestinal signs or symptoms or stools positive for occult blood at admission. The results of our study suggest that all patients with S. bovis septicemia need aggressive evaluation of the gastrointestinal tract, especially the colon."}
{"id": "PMID:484954", "title": "Inadvertent administration of intravenous fluids contaminated with fungus.", "content": "We present here the case of a patient inadvertently infused with intravenous fluid containing a \"fungus ball.\" Because little is known about such cases, we surveyed 113 infectious diseases specialists for their experiences and ideas on management. Seventy-six responded, of whom 18 had seen a total of 24 cases. Intravenous-fluid containers were often defective, and the fungi involved were usually \"contaminants.\" One patient developed significant fungal infection, and six others had transient symptoms temporally related to the infusion. Most specialists would administer antifungal therapy if symptoms developed, the patient was immunocompromised, or the fungus was pathogenic for humans.", "contents": "Inadvertent administration of intravenous fluids contaminated with fungus. We present here the case of a patient inadvertently infused with intravenous fluid containing a \"fungus ball.\" Because little is known about such cases, we surveyed 113 infectious diseases specialists for their experiences and ideas on management. Seventy-six responded, of whom 18 had seen a total of 24 cases. Intravenous-fluid containers were often defective, and the fungi involved were usually \"contaminants.\" One patient developed significant fungal infection, and six others had transient symptoms temporally related to the infusion. Most specialists would administer antifungal therapy if symptoms developed, the patient was immunocompromised, or the fungus was pathogenic for humans."}
{"id": "PMID:484955", "title": "Renal amyloidosis and subcutaneous drug abuse.", "content": "Two drug addicts who practiced subcutaneous injection of crushed tripelennamine and pentazocine tablets, a practice known as \"skin-popping,\" developed nephrotic syndrome and demonstrated amyloidosis on renal biopsy. These patients had chronic inflammation of the skin in the form of abscesses and ulcerations. Although both patients were long-standing drug abusers, they had practiced intradermal drug administration for only 3 years. The occurrence of amyloidosis in these patients appears to relate to their particular drug habir and must be considered in the differential diagnosis of proteinuria in this patient population.", "contents": "Renal amyloidosis and subcutaneous drug abuse. Two drug addicts who practiced subcutaneous injection of crushed tripelennamine and pentazocine tablets, a practice known as \"skin-popping,\" developed nephrotic syndrome and demonstrated amyloidosis on renal biopsy. These patients had chronic inflammation of the skin in the form of abscesses and ulcerations. Although both patients were long-standing drug abusers, they had practiced intradermal drug administration for only 3 years. The occurrence of amyloidosis in these patients appears to relate to their particular drug habir and must be considered in the differential diagnosis of proteinuria in this patient population."}
{"id": "PMID:484956", "title": "Extrahepatic biliary obstruction caused by metastatic breast carcinoma.", "content": "We describe our experience with seven patients who had extrahepatic biliary obstruction caused by metastatic breast cancer. The interval from the original diagnosis of breast cancer to the development of jaundice averaged 40 months, with a range of 9 months to 8 years. All patients were treated with surgical decompression, radiation, transhepatic catheter drainage, chemotherapy, or a combination of these modalities. Resolution of jaundice was achieved in six patients. Prolonged survival was realized in three; one patient lived for more than 6 years after surgical bypass, and two are alive and well at present (greater than 11 months after relief of biliary obstruction). Extrahepatic biliary obstruction by metastatic breast carcinoma should be distinguished from jaundice due to hepatic parenchymal destruction by this tumor; while the latter implies end-stage cancer, the former has the potential for significant palliation and prolonged survival.", "contents": "Extrahepatic biliary obstruction caused by metastatic breast carcinoma. We describe our experience with seven patients who had extrahepatic biliary obstruction caused by metastatic breast cancer. The interval from the original diagnosis of breast cancer to the development of jaundice averaged 40 months, with a range of 9 months to 8 years. All patients were treated with surgical decompression, radiation, transhepatic catheter drainage, chemotherapy, or a combination of these modalities. Resolution of jaundice was achieved in six patients. Prolonged survival was realized in three; one patient lived for more than 6 years after surgical bypass, and two are alive and well at present (greater than 11 months after relief of biliary obstruction). Extrahepatic biliary obstruction by metastatic breast carcinoma should be distinguished from jaundice due to hepatic parenchymal destruction by this tumor; while the latter implies end-stage cancer, the former has the potential for significant palliation and prolonged survival."}
{"id": "PMID:484957", "title": "Lack of cardiovascular effects of delta-9-tetrahydrocannabinol in chemically denervated men.", "content": "We have previously reported that 25 micrograms/kg of intravenous (i.v.) delta-9-tetrahydrocannabinol (delta-9-THC) produces marked increases in heart rate, prolongation of left ventricular ejection time corrected for heart rate (LVETc), and a shortening of the pre-ejection period in normal volunteers. Beta-adrenergic blockade partially attenuates these responses. To elucidate further the mechanism of action of delta-9-THC, we gave 10 normal volunteers 0.1 mg/kg of i.v. propranolol and 2 mg of i.v. atropine before they received 25 micrograms/kg of i.v. delta-9-THC. Systolic time intervals were compared in the denervated subjects before and after delta-9-THC. Post delta-9-THC responses were measured at a time approximating peak psychologic high. Mean +/- SEM heart rate before and after delta-9-THC was 89 +/- 4 and 87 +/- 3 beats/min (NS); mean +/- SEM pre-ejection period before and after delta-9-TCH was 107 +/- 5 and 109 +/- 4 ms (NS); and mean +/- SEM LVETc before and after delta-9-THC was 433 +/- 6 and 429 +/- 6 ms (NS). Since previous denervation of our subjects with atropine and propranolol totally abolished changes in heart rate and systolic time intervals, the cardiac effects of delta-9-THC appear to be mediated totally via the autonomic nervous system, probably reflecting direct central nervous system stimulation.", "contents": "Lack of cardiovascular effects of delta-9-tetrahydrocannabinol in chemically denervated men. We have previously reported that 25 micrograms/kg of intravenous (i.v.) delta-9-tetrahydrocannabinol (delta-9-THC) produces marked increases in heart rate, prolongation of left ventricular ejection time corrected for heart rate (LVETc), and a shortening of the pre-ejection period in normal volunteers. Beta-adrenergic blockade partially attenuates these responses. To elucidate further the mechanism of action of delta-9-THC, we gave 10 normal volunteers 0.1 mg/kg of i.v. propranolol and 2 mg of i.v. atropine before they received 25 micrograms/kg of i.v. delta-9-THC. Systolic time intervals were compared in the denervated subjects before and after delta-9-THC. Post delta-9-THC responses were measured at a time approximating peak psychologic high. Mean +/- SEM heart rate before and after delta-9-THC was 89 +/- 4 and 87 +/- 3 beats/min (NS); mean +/- SEM pre-ejection period before and after delta-9-TCH was 107 +/- 5 and 109 +/- 4 ms (NS); and mean +/- SEM LVETc before and after delta-9-THC was 433 +/- 6 and 429 +/- 6 ms (NS). Since previous denervation of our subjects with atropine and propranolol totally abolished changes in heart rate and systolic time intervals, the cardiac effects of delta-9-THC appear to be mediated totally via the autonomic nervous system, probably reflecting direct central nervous system stimulation."}
{"id": "PMID:484964", "title": "Mebendazole.", "content": "The broad-spectrum of activity and safety of mebendazole remain, after 5 years of clinical experience, unique features of this anthelmintic. Through microtubular destruction, mebendazole kills helminths by inhibiting glucose uptake into susceptible parasites. The drug's poor absorption does not appear to affect clinical efficacy except, perhaps, in the treatment of systemic helminth infections. Mebendazole is generally considered the drug of choice for trichuriasis and has therapeutic advantages over other anthelmintics in the treatment of enterobiasis and hookworm infections. Although mebendazole is an effective agent against ascariasis, there are preferable alternatives. Among its nonapproved uses, mebendazole shows great promise in the treatment of capillariasis and hydatid disease. Further investigation is needed to establish its role in the treatment of taeniasis, Hymenolepsis nana, strongyloidiasis, trichinosis, and Dipetalonema perstans. Undoubtedly, mebendazole will find its greatest value in the treatment of patients with multiple helminth infections.", "contents": "Mebendazole. The broad-spectrum of activity and safety of mebendazole remain, after 5 years of clinical experience, unique features of this anthelmintic. Through microtubular destruction, mebendazole kills helminths by inhibiting glucose uptake into susceptible parasites. The drug's poor absorption does not appear to affect clinical efficacy except, perhaps, in the treatment of systemic helminth infections. Mebendazole is generally considered the drug of choice for trichuriasis and has therapeutic advantages over other anthelmintics in the treatment of enterobiasis and hookworm infections. Although mebendazole is an effective agent against ascariasis, there are preferable alternatives. Among its nonapproved uses, mebendazole shows great promise in the treatment of capillariasis and hydatid disease. Further investigation is needed to establish its role in the treatment of taeniasis, Hymenolepsis nana, strongyloidiasis, trichinosis, and Dipetalonema perstans. Undoubtedly, mebendazole will find its greatest value in the treatment of patients with multiple helminth infections."}
{"id": "PMID:484965", "title": "Sensitivity and specificity of screening tests for renal vascular hypertension.", "content": "To facilitate the identification of patients with renal vascular hypertension, we evaluated four potential screening tests: rapid-sequence urography, systolic-diastolic abdominal bruit, upright plasma renin activity (PRA), and response to saralasin infusion. Our study included 379 normal subjects, 199 essential hypertensive patients with normal renal angiograms, and 64 patients with surgically responsive renal vascular hypertension. Thirty-nine percent of patients with renal vascular hypertension had systolic-diastolic bruits, 76% abnormal urograms, and 27% a PRA greater than 30 ng of angiotensin 1/mL.3 h. Only one half of the 23 patients with renal vascular hypertension tested had a depressor response to saralasin, as did two of 13 essential hypertensive patients. In essential hypertensive subjects, 1% had systolic-diastolic bruits, 2% abnormal urograms, and 5% upright renin values greater than 30 ng of angiotensin 1/mL.3 h. The screening combination of urogram, bruit, or upright renin value offered a test sensitivity of 93%, with a specificity of 92%. The results of saralasin infusion failed to increase the diagnostic yield.", "contents": "Sensitivity and specificity of screening tests for renal vascular hypertension. To facilitate the identification of patients with renal vascular hypertension, we evaluated four potential screening tests: rapid-sequence urography, systolic-diastolic abdominal bruit, upright plasma renin activity (PRA), and response to saralasin infusion. Our study included 379 normal subjects, 199 essential hypertensive patients with normal renal angiograms, and 64 patients with surgically responsive renal vascular hypertension. Thirty-nine percent of patients with renal vascular hypertension had systolic-diastolic bruits, 76% abnormal urograms, and 27% a PRA greater than 30 ng of angiotensin 1/mL.3 h. Only one half of the 23 patients with renal vascular hypertension tested had a depressor response to saralasin, as did two of 13 essential hypertensive patients. In essential hypertensive subjects, 1% had systolic-diastolic bruits, 2% abnormal urograms, and 5% upright renin values greater than 30 ng of angiotensin 1/mL.3 h. The screening combination of urogram, bruit, or upright renin value offered a test sensitivity of 93%, with a specificity of 92%. The results of saralasin infusion failed to increase the diagnostic yield."}
{"id": "PMID:484966", "title": "The medical record as a basis for assessing physician competence.", "content": "The medical record can be used for evaluating physician performance. Within the limitations described, it is an essential source of information on the delivery of care as well as a measure of proficiency. The process of care should be particularly emphasized, without neglecting appropriate outcome measures. The confounding of outcome measures by compliance factors, natural history of the disease process, severity of illness, status of defense mechanisms, performance of ancillary services, and personnel does not make this an attractive single measure of physician performance. Knowledge assessment and performance assessment play a complementary role; both are necessary to identify competency. The potential for improving medical care and the recording of essential findings by such an emphasis on individual assessment based on medical records is enormous. Technologic progress in recording and retrieving medical information is facilitating use of the record to measure proficiency. A carefully structured blend of cognitive examination to establish a knowledge base and review of the medical record to measure performance will provide better measures of competency.", "contents": "The medical record as a basis for assessing physician competence. The medical record can be used for evaluating physician performance. Within the limitations described, it is an essential source of information on the delivery of care as well as a measure of proficiency. The process of care should be particularly emphasized, without neglecting appropriate outcome measures. The confounding of outcome measures by compliance factors, natural history of the disease process, severity of illness, status of defense mechanisms, performance of ancillary services, and personnel does not make this an attractive single measure of physician performance. Knowledge assessment and performance assessment play a complementary role; both are necessary to identify competency. The potential for improving medical care and the recording of essential findings by such an emphasis on individual assessment based on medical records is enormous. Technologic progress in recording and retrieving medical information is facilitating use of the record to measure proficiency. A carefully structured blend of cognitive examination to establish a knowledge base and review of the medical record to measure performance will provide better measures of competency."}
{"id": "PMID:484967", "title": "The recent decline in ischemic heart disease mortality.", "content": "Age-adjusted overall ischemic heart disease mortality declined by 20.7% in the United States between 1968 and 1976. Declines were recorded in both sexes, in all age groups, and in three major race/ethnic groups. The decline cannot be explained by shifts in assignment of cause of death on death certificates. Available evidence suggests that improvements in diet with concomitant declines in serum cholesterol concentration, decreased cigarette smoking, improved hypertension control, and possibly increased leisure-time physical exercise explain a portion of the decline. Coronary care units have probably also contributed. Emergency medical services and coronary bypass surgery were not widely enough applied during this period to have contributed to the decline. Several strategies are suggested for further elucidating the relative contributions of various preventive and curative programs. Such information is urgently needed to foster optimum allocation of scarce resources between competing (and expensive) programs.", "contents": "The recent decline in ischemic heart disease mortality. Age-adjusted overall ischemic heart disease mortality declined by 20.7% in the United States between 1968 and 1976. Declines were recorded in both sexes, in all age groups, and in three major race/ethnic groups. The decline cannot be explained by shifts in assignment of cause of death on death certificates. Available evidence suggests that improvements in diet with concomitant declines in serum cholesterol concentration, decreased cigarette smoking, improved hypertension control, and possibly increased leisure-time physical exercise explain a portion of the decline. Coronary care units have probably also contributed. Emergency medical services and coronary bypass surgery were not widely enough applied during this period to have contributed to the decline. Several strategies are suggested for further elucidating the relative contributions of various preventive and curative programs. Such information is urgently needed to foster optimum allocation of scarce resources between competing (and expensive) programs."}
{"id": "PMID:484986", "title": "[Endomyocardial biopsy results and left ventricular function in primary myocardiopathies (author's transl)].", "content": "Endomyocardial biopsies (EMB) of the left ventricle (LV) were performed during cardiac catheterization in 27 patients with apparently primary myocardiopathies (PMC). The results of optical and electron microscopy were compared with ventricular performance indices calculated from hemodynamic and cinematographic data from the left ventricle. The telediastolic pressure of the LV increases with the spread of fibrosis (r = 0.68) suggesting that it disturbs the filling of this cavity. Systolic effort is reduced as this fibrosis increases (r = 0.56), and it contributes to the changes in the pumping function of the LV, but the contractile power of the LV is altered in a less evident and less constant manner. The association of cell and fibro-elastic tissue degeneration is always accompanied by a drop in ventricular performance (p less than 0.0001). Apart from its diagnostic role, which is limited but may be of great value, the EMB can help to clarify the physiopathology of PMC and to formulate a prognosis based on more solid grounds.", "contents": "[Endomyocardial biopsy results and left ventricular function in primary myocardiopathies (author's transl)]. Endomyocardial biopsies (EMB) of the left ventricle (LV) were performed during cardiac catheterization in 27 patients with apparently primary myocardiopathies (PMC). The results of optical and electron microscopy were compared with ventricular performance indices calculated from hemodynamic and cinematographic data from the left ventricle. The telediastolic pressure of the LV increases with the spread of fibrosis (r = 0.68) suggesting that it disturbs the filling of this cavity. Systolic effort is reduced as this fibrosis increases (r = 0.56), and it contributes to the changes in the pumping function of the LV, but the contractile power of the LV is altered in a less evident and less constant manner. The association of cell and fibro-elastic tissue degeneration is always accompanied by a drop in ventricular performance (p less than 0.0001). Apart from its diagnostic role, which is limited but may be of great value, the EMB can help to clarify the physiopathology of PMC and to formulate a prognosis based on more solid grounds."}
{"id": "PMID:484988", "title": "[Severe aortic incompetence from dysplasia of the valve. A report on 19 cases of this real but often unrecognized disorder (author's transl)].", "content": "A total of 19 patients were found to have severe aortic incompetence from dysplasia of the valve (thin, spread-out valves having a tendency towards eversion). This type of valve abnormality is often unrecognized but is found in 13,6% of cases of severe aortic incompetence. The relationship between this condition and Marfan's syndrome or its aberrant forms are discussed, as well as its frequency when unrelated to this disease. The dysplasias can be isolated phenomena or associated with pathological conditions of the ascending aorta. The results of this review suggest that some of these dysplasias might be rheumatic in origin.", "contents": "[Severe aortic incompetence from dysplasia of the valve. A report on 19 cases of this real but often unrecognized disorder (author's transl)]. A total of 19 patients were found to have severe aortic incompetence from dysplasia of the valve (thin, spread-out valves having a tendency towards eversion). This type of valve abnormality is often unrecognized but is found in 13,6% of cases of severe aortic incompetence. The relationship between this condition and Marfan's syndrome or its aberrant forms are discussed, as well as its frequency when unrelated to this disease. The dysplasias can be isolated phenomena or associated with pathological conditions of the ascending aorta. The results of this review suggest that some of these dysplasias might be rheumatic in origin."}
{"id": "PMID:484989", "title": "[Clinical and biological aspects of macroamylasemia (author's transl)].", "content": "The authors report a case of macroamylasemia in which the diagnosis was made with some difficulty. A hyperamylasemia was discovered after the patient, an alcoholic, had been hospitalized for atypical abdominal pain and weight loss, and this was thought to be due to an acute episode of chronic pancreatitis. The absence of an incrase in amylasuria suggested the presence of a macroamylasemia, and both diagnoses were confirmed by suitable exploratory investigations. Alcoholic cirrhosis was also present. The two main known types of macroamylase and their iatrogenic variant are described as well as the incidence of this biological anomaly in the general population. Confirming the presence of this anomaly in the plasma is a delicate and complex procedure. Simultaneous study of amylase-creatinine clearance ratio was thought to be a decisive test, but this does not appear to be true.", "contents": "[Clinical and biological aspects of macroamylasemia (author's transl)]. The authors report a case of macroamylasemia in which the diagnosis was made with some difficulty. A hyperamylasemia was discovered after the patient, an alcoholic, had been hospitalized for atypical abdominal pain and weight loss, and this was thought to be due to an acute episode of chronic pancreatitis. The absence of an incrase in amylasuria suggested the presence of a macroamylasemia, and both diagnoses were confirmed by suitable exploratory investigations. Alcoholic cirrhosis was also present. The two main known types of macroamylase and their iatrogenic variant are described as well as the incidence of this biological anomaly in the general population. Confirming the presence of this anomaly in the plasma is a delicate and complex procedure. Simultaneous study of amylase-creatinine clearance ratio was thought to be a decisive test, but this does not appear to be true."}
{"id": "PMID:484992", "title": "A code for reporting and comparing results in phage typing.", "content": "This code, like the others proposed, involves the definition of a characteristic reaction for each system. Each reaction must be positive or negative. As regards the interpretation of results, it is worth noting that if the bacteriophage typing system uses not less than 5 phages, there is no problem for interpreting the results of small series of 10 to 20 strains, whatever the code used. But should the system use more than 5 phages, the advantage offered by the octal code becomes obvious because, without computer, the manual coding for comparing the results of various series is always possible. Beyond 6 phages, the binary pattern reporting, unintelligible without correction, is possible with an octal number not exceeding 7 digits for a pattern corresponding to a 21-phage set. When very large series of strains are considered, the coding can be completely done and printed out by any computer through a very simple program. This proposed code is not only rational but also a useful method even if the number of phages used is larger than 20 or 30, but after a computer becomes necessary however the coding system used.", "contents": "A code for reporting and comparing results in phage typing. This code, like the others proposed, involves the definition of a characteristic reaction for each system. Each reaction must be positive or negative. As regards the interpretation of results, it is worth noting that if the bacteriophage typing system uses not less than 5 phages, there is no problem for interpreting the results of small series of 10 to 20 strains, whatever the code used. But should the system use more than 5 phages, the advantage offered by the octal code becomes obvious because, without computer, the manual coding for comparing the results of various series is always possible. Beyond 6 phages, the binary pattern reporting, unintelligible without correction, is possible with an octal number not exceeding 7 digits for a pattern corresponding to a 21-phage set. When very large series of strains are considered, the coding can be completely done and printed out by any computer through a very simple program. This proposed code is not only rational but also a useful method even if the number of phages used is larger than 20 or 30, but after a computer becomes necessary however the coding system used."}
{"id": "PMID:484993", "title": "[The influence of thymus on mouse resistance against rabies before and after vaccination (author's transl)].", "content": "Thymus participation was investigated in a comparative study of natural resistance to rabies in Nude mice and normal litter mates. Such participation does not seem to happen during Challenge Virus Strain infection but might change the host cell-virus metabolism during street rabies infection by inhibition of Negri bodies genesis. However, this participation seems indispensable (except in few cases) to allow resistance after vaccination. For instance twice more antigen is necessary to immunize thymectomized than normal mice, and most of Nude mice can not stand intramuscular challenge nor produce antibodies, neither they are able to be protected by injection of antirabies antibodies.", "contents": "[The influence of thymus on mouse resistance against rabies before and after vaccination (author's transl)]. Thymus participation was investigated in a comparative study of natural resistance to rabies in Nude mice and normal litter mates. Such participation does not seem to happen during Challenge Virus Strain infection but might change the host cell-virus metabolism during street rabies infection by inhibition of Negri bodies genesis. However, this participation seems indispensable (except in few cases) to allow resistance after vaccination. For instance twice more antigen is necessary to immunize thymectomized than normal mice, and most of Nude mice can not stand intramuscular challenge nor produce antibodies, neither they are able to be protected by injection of antirabies antibodies."}
{"id": "PMID:484994", "title": "IgM and IgG antibody responses in rabies encephalitis.", "content": "Immunological survey of 3 patients with proved rabies encephalitis shows three interesting facts. 1) An IgM local synthesis, sometimes proportionally higher than IgG local synthesis, is observed in 8 CSF (among 13 investigated) and detected early, during the first week of the disease for 2 patients. 2) A relative poor IgG response is noted; this response is absent in one and decreasing in the 2 others patients despite their progressive aggravation. 3) A very little elevation of blood nucleic antibodies is detected in 5 CSF only. This very poor synthesis of antinucleic IgG contrast with their common increase in viral encephalitis. Thus, a viral immunosuppression may be discussed in rabies encephalitis, which may contribute to the lethal prognosis.", "contents": "IgM and IgG antibody responses in rabies encephalitis. Immunological survey of 3 patients with proved rabies encephalitis shows three interesting facts. 1) An IgM local synthesis, sometimes proportionally higher than IgG local synthesis, is observed in 8 CSF (among 13 investigated) and detected early, during the first week of the disease for 2 patients. 2) A relative poor IgG response is noted; this response is absent in one and decreasing in the 2 others patients despite their progressive aggravation. 3) A very little elevation of blood nucleic antibodies is detected in 5 CSF only. This very poor synthesis of antinucleic IgG contrast with their common increase in viral encephalitis. Thus, a viral immunosuppression may be discussed in rabies encephalitis, which may contribute to the lethal prognosis."}
{"id": "PMID:484990", "title": "[Rahnella aquatilis, a new member of the Enterobacteriaceae (author's transl)].", "content": "A DNA-DNA hybridization study was carried out to determine the taxonomic position of a new group of enterobacteria (group H2) previously studied by numerical taxonomy. All the strains of this group revealed relatively high reassociation binding ratios with the centrotype; 82% of the strains of the group showed more than 69% of reassociation with the centrotype. In spite of numerical taxonomy conclusions, there was no genetic relationship with the species Enterobacter cloacae (higher reassociation binding ratio: 37%). No significant genetic relationship with the other groups of enterobacteria was found. Rahnella aquatilis was defined from phenotypic and genetic data. The strain 133 (CIP 78-65) is proposed as type strain of the species.", "contents": "[Rahnella aquatilis, a new member of the Enterobacteriaceae (author's transl)]. A DNA-DNA hybridization study was carried out to determine the taxonomic position of a new group of enterobacteria (group H2) previously studied by numerical taxonomy. All the strains of this group revealed relatively high reassociation binding ratios with the centrotype; 82% of the strains of the group showed more than 69% of reassociation with the centrotype. In spite of numerical taxonomy conclusions, there was no genetic relationship with the species Enterobacter cloacae (higher reassociation binding ratio: 37%). No significant genetic relationship with the other groups of enterobacteria was found. Rahnella aquatilis was defined from phenotypic and genetic data. The strain 133 (CIP 78-65) is proposed as type strain of the species."}
{"id": "PMID:484995", "title": "[Detection and significance of histidine decarboxylase (HDC) in \"Klebsiella\", \"Enterobacter\" and \"Serratia\" (author's transl)].", "content": "A quick colorimetric procedure for detection of histidine decarboxylase (HDC) in cultures of Klebsiella, Enterobacter and Serratia is described. Only E. aerogenes could decarboxylate histidine (production of histamine). The interest of HDC test for differentiation of E. aerogenes from K. pneumoniae (especially urease negative strains) is discussed.", "contents": "[Detection and significance of histidine decarboxylase (HDC) in \"Klebsiella\", \"Enterobacter\" and \"Serratia\" (author's transl)]. A quick colorimetric procedure for detection of histidine decarboxylase (HDC) in cultures of Klebsiella, Enterobacter and Serratia is described. Only E. aerogenes could decarboxylate histidine (production of histamine). The interest of HDC test for differentiation of E. aerogenes from K. pneumoniae (especially urease negative strains) is discussed."}
{"id": "PMID:484996", "title": "Local steroid therapy in adnexal hemangioma.", "content": "Hemangiomas of the eyelid and adnexa in infants may require treatment if they threaten to induce amblyopia or motility disturbances. Four cases are presented that were treated with local injection of corticosteroids. Three responded favorably. Advantages over other modes of treatment are discussed.", "contents": "Local steroid therapy in adnexal hemangioma. Hemangiomas of the eyelid and adnexa in infants may require treatment if they threaten to induce amblyopia or motility disturbances. Four cases are presented that were treated with local injection of corticosteroids. Three responded favorably. Advantages over other modes of treatment are discussed."}
{"id": "PMID:484991", "title": "[A study of the action of sodium chlorate on strains of nitrate reducing soil bacteria (author's transl)].", "content": "Fours strains of nitrate reducing bacteria isolated from soil were studied for their behavior towards chlorate. They are facultative anaerobes, except for Bacillus megatherium (which is a strict aerobe) and they possess a nitrate reductase A. The growth of three strains of bacteria (Klebsiella pneumoniae, B. licheniformis and Micromonospora globosa) was slowed by sodium chlorate at a concentration of 0.06 to 0.1% while the other strain (B. megatherium) tolerated the CIO3- well. The delay of bacterial growth due to chlorate lasts for a certain period, after which the bacteria multiply again. The lag phase is due to small quantities of chlorite produced from the chlorate; the growth phase which follows is provoked by the multiplication of chlorate resistant mutants, most often nitrate reductase-negative and sometimes positive. Some reverse mutants nitrate reductase positive of K. pneumoniae no longer had the same characteristics as the wild strain: some resisted to chlorate or were different as to gas formation. The reduction of nitrate to ammonia by these bacteria is diminished in the presence of chlorate: the reduction of nitrate to nitrite was inhibited or not inhibited according to the type of strain. The bacteria broke down the chlorate partially or completely, according to the strains and the sustrates.", "contents": "[A study of the action of sodium chlorate on strains of nitrate reducing soil bacteria (author's transl)]. Fours strains of nitrate reducing bacteria isolated from soil were studied for their behavior towards chlorate. They are facultative anaerobes, except for Bacillus megatherium (which is a strict aerobe) and they possess a nitrate reductase A. The growth of three strains of bacteria (Klebsiella pneumoniae, B. licheniformis and Micromonospora globosa) was slowed by sodium chlorate at a concentration of 0.06 to 0.1% while the other strain (B. megatherium) tolerated the CIO3- well. The delay of bacterial growth due to chlorate lasts for a certain period, after which the bacteria multiply again. The lag phase is due to small quantities of chlorite produced from the chlorate; the growth phase which follows is provoked by the multiplication of chlorate resistant mutants, most often nitrate reductase-negative and sometimes positive. Some reverse mutants nitrate reductase positive of K. pneumoniae no longer had the same characteristics as the wild strain: some resisted to chlorate or were different as to gas formation. The reduction of nitrate to ammonia by these bacteria is diminished in the presence of chlorate: the reduction of nitrate to nitrite was inhibited or not inhibited according to the type of strain. The bacteria broke down the chlorate partially or completely, according to the strains and the sustrates."}
{"id": "PMID:484997", "title": "Intravitreal and intracameral cysticercosis.", "content": "A 49-year-old Mexican male presented with a free-floating cyst in the vitreous. The cyst was removed at the time of cataract surgery and on the first postoperative day a second cyst was found in the anterior chamber. The second cyst was excised by cryoextraction 6 weeks after the initial surgery, but the eye developed an inoperable retinal detachment and phthisis bulbi. Although the diagnosis of cysticercosis was made clinically, initially, the only laboratory evidence for parasitic infection was a peripheral blood eosinophilia. The patient later developed an enlarged liver which was consistent with parasitic infection based on a liver scan. The morphology and life cycle of the parasite is described as well as suggestions for surgical removal.", "contents": "Intravitreal and intracameral cysticercosis. A 49-year-old Mexican male presented with a free-floating cyst in the vitreous. The cyst was removed at the time of cataract surgery and on the first postoperative day a second cyst was found in the anterior chamber. The second cyst was excised by cryoextraction 6 weeks after the initial surgery, but the eye developed an inoperable retinal detachment and phthisis bulbi. Although the diagnosis of cysticercosis was made clinically, initially, the only laboratory evidence for parasitic infection was a peripheral blood eosinophilia. The patient later developed an enlarged liver which was consistent with parasitic infection based on a liver scan. The morphology and life cycle of the parasite is described as well as suggestions for surgical removal."}
{"id": "PMID:485000", "title": "Pattern of retinal diseases in Lagos.", "content": "Although primary retinal detachments occur infrequently in Africans, retinal diseases are not so uncommon. Ninety-two out of 1,723 patients seen in the Lagos University Teaching Hospital Eye Clinic had retinal diseases, a prevalence of 5.3%. We have seen most of the diseases described in other countries but while sicklemia retinopathy and various types of tapetoretinal dystrophy constitute a problem, hypertensive and diabetic retinopathies are infrequently seen. Retinoblastoma is the only tumor encountered so far and would appear to be sporadic. Positive family histories are difficult to obtain in our patients. It is anticipated that more retinal diseases will be diagnosed as more people become literate and notice slight to moderate visual loss.", "contents": "Pattern of retinal diseases in Lagos. Although primary retinal detachments occur infrequently in Africans, retinal diseases are not so uncommon. Ninety-two out of 1,723 patients seen in the Lagos University Teaching Hospital Eye Clinic had retinal diseases, a prevalence of 5.3%. We have seen most of the diseases described in other countries but while sicklemia retinopathy and various types of tapetoretinal dystrophy constitute a problem, hypertensive and diabetic retinopathies are infrequently seen. Retinoblastoma is the only tumor encountered so far and would appear to be sporadic. Positive family histories are difficult to obtain in our patients. It is anticipated that more retinal diseases will be diagnosed as more people become literate and notice slight to moderate visual loss."}
{"id": "PMID:485002", "title": "Ocular manifestations after upper dorsal sympathectomy.", "content": "Fifty patients underwent ophthalmologic examination before and after bilateral upper dorsal sympathectomy performed for palmar hyperhidrosis. Postoperative examination was done during the first week, at 3 to 4 weeks and at 6 to 12 months after the operation. Immediately after surgery there were 18 patients with ptosis (6 severe) and 23 with miosis (12 severe). There were 19 patients with excessive lacrimation and some degree of congestion of conjunctival blood vessels was present in almost all patients. Uniocular decrease in tears was present in 2 patients. Most of these manifestations decreased with passage of time. At one year there remained only 3 patients with severe ptosis and 4 with severe miosis. The results suggest that the classical anatomic explanation of Horner's syndrome may require some modification.", "contents": "Ocular manifestations after upper dorsal sympathectomy. Fifty patients underwent ophthalmologic examination before and after bilateral upper dorsal sympathectomy performed for palmar hyperhidrosis. Postoperative examination was done during the first week, at 3 to 4 weeks and at 6 to 12 months after the operation. Immediately after surgery there were 18 patients with ptosis (6 severe) and 23 with miosis (12 severe). There were 19 patients with excessive lacrimation and some degree of congestion of conjunctival blood vessels was present in almost all patients. Uniocular decrease in tears was present in 2 patients. Most of these manifestations decreased with passage of time. At one year there remained only 3 patients with severe ptosis and 4 with severe miosis. The results suggest that the classical anatomic explanation of Horner's syndrome may require some modification."}
{"id": "PMID:485004", "title": "Blood levels of thiamine and ascorbic acid in chronic open-angle glaucoma.", "content": "Blood levels of thiamine and ascorbic acid in chronic open-angle glaucoma are determined in this study. Dietary vitamin intake was compared with thiamine and ascorbic acid blood levels in a sample of 38 patients with glaucoma and 12 controls. These patients had a statistically significant lower thiamine blood level than controls (P less than 0.001), but no significant difference was found for ascorbic acid blood levels. Poor absorption of thiamine occurred in the glaucomatous patients in this study.", "contents": "Blood levels of thiamine and ascorbic acid in chronic open-angle glaucoma. Blood levels of thiamine and ascorbic acid in chronic open-angle glaucoma are determined in this study. Dietary vitamin intake was compared with thiamine and ascorbic acid blood levels in a sample of 38 patients with glaucoma and 12 controls. These patients had a statistically significant lower thiamine blood level than controls (P less than 0.001), but no significant difference was found for ascorbic acid blood levels. Poor absorption of thiamine occurred in the glaucomatous patients in this study."}
{"id": "PMID:485005", "title": "\"Congenital\" hematic cyst of the orbit.", "content": "A case of true hematic cyst of the orbit in a 10-year-old girl, apparently resulting from birth trauma, is presented. Ultrasonic and clinical features believed to aid the examiner in correctly diagnosing such a lesion are presented. While apparently unique among reported cases, \"congenital\" hematic cysts of the orbit undoubtedly occur occasionally and the possibility of this entity should be considered in appropriate circumstances.", "contents": "\"Congenital\" hematic cyst of the orbit. A case of true hematic cyst of the orbit in a 10-year-old girl, apparently resulting from birth trauma, is presented. Ultrasonic and clinical features believed to aid the examiner in correctly diagnosing such a lesion are presented. While apparently unique among reported cases, \"congenital\" hematic cysts of the orbit undoubtedly occur occasionally and the possibility of this entity should be considered in appropriate circumstances."}
{"id": "PMID:485006", "title": "Choroidal detachment with flat anterior chamber after cyclocryotherapy.", "content": "A 50-year-old black man with uncontrolled open-angle glaucoma developed a flat anterior chamber after cyclocryotherapy. Because of poor visualization secondary to a cataract, ultrasonography was used to confirm the diagnosis of a large choroidal detachment. After suprachoroidal drainage and reformation of the anterior chamber, the patient has done well.", "contents": "Choroidal detachment with flat anterior chamber after cyclocryotherapy. A 50-year-old black man with uncontrolled open-angle glaucoma developed a flat anterior chamber after cyclocryotherapy. Because of poor visualization secondary to a cataract, ultrasonography was used to confirm the diagnosis of a large choroidal detachment. After suprachoroidal drainage and reformation of the anterior chamber, the patient has done well."}
{"id": "PMID:485007", "title": "Sympathetic ophthalmitis simulating Vogt-Koyanagi-Harada's disease after retinal detachment surgery.", "content": "A rare case is reported of a 49-year-old myopic patient who developed sympathetic ophthalmitis simulating Vogt-Koyanagi-Harada's disease following successful retinal detachment surgery, who showed satisfactory response to systemic and subconjunctival steroids and was saved from permanent blindness.", "contents": "Sympathetic ophthalmitis simulating Vogt-Koyanagi-Harada's disease after retinal detachment surgery. A rare case is reported of a 49-year-old myopic patient who developed sympathetic ophthalmitis simulating Vogt-Koyanagi-Harada's disease following successful retinal detachment surgery, who showed satisfactory response to systemic and subconjunctival steroids and was saved from permanent blindness."}
{"id": "PMID:485008", "title": "[Nasosinusal polyposis and aspirin intolerance. Fernand Widal-Lermoyez syndrome].", "content": "The authors describe the clinical picture of the aspirin idiosyncrasy and propose to call this peculiar entity: syndrom of Widal and Lermoyez. They compare 25 cases of aspirin nasal polyposis with 26 other cases of various etiologies. Other substances than aspirin seem to be charged. The complications are regular with severe asthma and infection. The pathogenesis is discussed, excluding an allergic mechanism; it remain not quite clear. Essentially prophylactic, the treatment is poor and difficult.", "contents": "[Nasosinusal polyposis and aspirin intolerance. Fernand Widal-Lermoyez syndrome]. The authors describe the clinical picture of the aspirin idiosyncrasy and propose to call this peculiar entity: syndrom of Widal and Lermoyez. They compare 25 cases of aspirin nasal polyposis with 26 other cases of various etiologies. Other substances than aspirin seem to be charged. The complications are regular with severe asthma and infection. The pathogenesis is discussed, excluding an allergic mechanism; it remain not quite clear. Essentially prophylactic, the treatment is poor and difficult."}
{"id": "PMID:485013", "title": "[Anal symptoms of gastro-intestinal diseases].", "content": "In most cases the ano-cutaneous clinical symptoms correlated to diseases of the gastro-intestinal tract are not specific (erythema, itching, wounds or scarring). However in the following diseases occasional dermatological lesions may directly contribute to their diagnosis: in Crohn's disease, tuberculosis of bowel, chronic entamoebiasis and bilharziosis, the skin lesions of the anal area have the same histological structure as the gut lesions. Perianal fistulas and ulcers are frequent in Crohn's disease especially if there is a colonic and rectal spreading; they respond badly to steroid therapy and are often correlated with a worse prognosis. Perianal specific lesions occur often in oxyuriasis in children, in candidiasis of the digestive tract, in systemic aphthosis and in some malignancies. In other gastro-intestinal disturbances, the dermatological and features are less specific and can only be suggestive: iatrogenic and microbial diarrheas, side-effects of laxatives, proctological diseases. It has to be emphasized that pruritus ani is only induced by deeper lesions when they spread to the perianal skin. In proctological practice, contact dermatitis by sensitivity to anaesthetics or suppository balsams (Peruvian balsam), itching or burning atrophy by topical steroid abuse, non-diagnosed fungal (candidiasis), bacterial (erythrasma) or psoriatic intertrigos (flexural psoriasis) may sometimes explain the failure of therapy.", "contents": "[Anal symptoms of gastro-intestinal diseases]. In most cases the ano-cutaneous clinical symptoms correlated to diseases of the gastro-intestinal tract are not specific (erythema, itching, wounds or scarring). However in the following diseases occasional dermatological lesions may directly contribute to their diagnosis: in Crohn's disease, tuberculosis of bowel, chronic entamoebiasis and bilharziosis, the skin lesions of the anal area have the same histological structure as the gut lesions. Perianal fistulas and ulcers are frequent in Crohn's disease especially if there is a colonic and rectal spreading; they respond badly to steroid therapy and are often correlated with a worse prognosis. Perianal specific lesions occur often in oxyuriasis in children, in candidiasis of the digestive tract, in systemic aphthosis and in some malignancies. In other gastro-intestinal disturbances, the dermatological and features are less specific and can only be suggestive: iatrogenic and microbial diarrheas, side-effects of laxatives, proctological diseases. It has to be emphasized that pruritus ani is only induced by deeper lesions when they spread to the perianal skin. In proctological practice, contact dermatitis by sensitivity to anaesthetics or suppository balsams (Peruvian balsam), itching or burning atrophy by topical steroid abuse, non-diagnosed fungal (candidiasis), bacterial (erythrasma) or psoriatic intertrigos (flexural psoriasis) may sometimes explain the failure of therapy."}
{"id": "PMID:485009", "title": "[Pharyngolplasty by transposition of the posterior tonsillar pillar (author's transl)].", "content": "13 children suffering from velo-pharyngeal insufficiency were operated. A transposition of the posterior pillar was carried out. After exposing the principle of the method, the anatomical bases of the operation and the operation technique used are described. The pharyngeal sphincter thus reconstituted has nearly always resulted in an improvement in the phonation in these subjects.", "contents": "[Pharyngolplasty by transposition of the posterior tonsillar pillar (author's transl)]. 13 children suffering from velo-pharyngeal insufficiency were operated. A transposition of the posterior pillar was carried out. After exposing the principle of the method, the anatomical bases of the operation and the operation technique used are described. The pharyngeal sphincter thus reconstituted has nearly always resulted in an improvement in the phonation in these subjects."}
{"id": "PMID:485014", "title": "[Household cleansers and nickel allergies].", "content": "Attention of authors has been drawn by the observation of patients presenting a nickel-dermatosis, instigating by the touch of jewelry (ear-rings, bracelets, necklaces...) and also a sensitization for housework cleansing agents. The chemical analysis of numerous housework cleansing agents exhibited the constant presence of nickel in these housework cleansings. These investigations enable the authors to affirm a close relationship between sensitization for nickel and sensitization for housework cleansings. Some patients present also sensitization for chromium or cobalt. The authors think this polysensitization is induced by metal's contamination.", "contents": "[Household cleansers and nickel allergies]. Attention of authors has been drawn by the observation of patients presenting a nickel-dermatosis, instigating by the touch of jewelry (ear-rings, bracelets, necklaces...) and also a sensitization for housework cleansing agents. The chemical analysis of numerous housework cleansing agents exhibited the constant presence of nickel in these housework cleansings. These investigations enable the authors to affirm a close relationship between sensitization for nickel and sensitization for housework cleansings. Some patients present also sensitization for chromium or cobalt. The authors think this polysensitization is induced by metal's contamination."}
{"id": "PMID:485015", "title": "[Dermatitis herpetiformis (author's transl)].", "content": "Detailed investigations of 9 patients with dermatitis herpetiformis are presented. In all cases cutaneous lesions were controlled by dapsone alone or by dapsone and gluten free diet. Granular IgA deposits were found in 7 patients, linear IgA deposits in one, and C3 component of complement in one. 3 patients out of 8 tested, carried the specific HLA-B8 antigen. Despite an extensive investigation, no malabsorption was detected. Jejunal biopsies were performed in 8 cases. Jejunal villous flattening was observed in one patient. It improved after a 2 months gluten free diet on subsequent jejunal biopsies. D. H. seems peculiar in France as compared with case reports from other countries: low prevalence of gluten sensitive enteropathy; rare occurrence of the specific HLA-B8 antigen; incidence of D. H. seems to be low in France. It is noticeable that french incidence of coeliac disease is low as well. This suggests a genetic difference in the investigated population (low prevalence of HLA-B8 antigen) and/or different alimentary habits, particularly a low dietary gluten amount.", "contents": "[Dermatitis herpetiformis (author's transl)]. Detailed investigations of 9 patients with dermatitis herpetiformis are presented. In all cases cutaneous lesions were controlled by dapsone alone or by dapsone and gluten free diet. Granular IgA deposits were found in 7 patients, linear IgA deposits in one, and C3 component of complement in one. 3 patients out of 8 tested, carried the specific HLA-B8 antigen. Despite an extensive investigation, no malabsorption was detected. Jejunal biopsies were performed in 8 cases. Jejunal villous flattening was observed in one patient. It improved after a 2 months gluten free diet on subsequent jejunal biopsies. D. H. seems peculiar in France as compared with case reports from other countries: low prevalence of gluten sensitive enteropathy; rare occurrence of the specific HLA-B8 antigen; incidence of D. H. seems to be low in France. It is noticeable that french incidence of coeliac disease is low as well. This suggests a genetic difference in the investigated population (low prevalence of HLA-B8 antigen) and/or different alimentary habits, particularly a low dietary gluten amount."}
{"id": "PMID:485010", "title": "[Typanosclerosis and labyrinthine trephination. Results].", "content": "38 recent cases of tympanosclerosis have been treated by labyrinthotomy with a few number of complications of this surgery is realised on a dry ear. A platinectomy covered by a vein graft must be prefered at a simple exfoliation of tympanosclerosis covering the fooplaste if the annular ligament is not perfect. The best results are get by direct prothesis between ear drum and vein graft covering platinectomy.", "contents": "[Typanosclerosis and labyrinthine trephination. Results]. 38 recent cases of tympanosclerosis have been treated by labyrinthotomy with a few number of complications of this surgery is realised on a dry ear. A platinectomy covered by a vein graft must be prefered at a simple exfoliation of tympanosclerosis covering the fooplaste if the annular ligament is not perfect. The best results are get by direct prothesis between ear drum and vein graft covering platinectomy."}
{"id": "PMID:485016", "title": "[Richner-Hanhart's syndrome or oculo-cutaneous tyrosinosis (about one case) (author's transl)].", "content": "Richner-Hanhart's syndrome correspond to an hypertyrosinemia due to a deficiency of a soluble tyrosine amino-transferase. This recently described tyrosinosis has been called oculo-cutaneous tyrosinosis. This disease transmitted on a recessive way in amenable to a treatment by a low tyrosine diet. In an infant, 18 months old, presenting a bilateral dendritic keratitis, a punctiform keratosis of the extremities, a patchy leucokeratosis of the tongue and a mental ketardation, the hypertyrosinemia reached 52 mg per 100 ml and the urine demonstrated the presence of phenyl-atonic acids. There was no hepato-renal involvement. The deferency of soluble amino-transferase was studied on the hepatocytes and confirmed. The low tyrosine diet made the clinical and biological signs disappear. The improvement was noticeable from the first week on and continued during the 16 months of the follow-up. There was no ill effect of the special diet on the weight and height growth. The oculo-cutaneous tyrosinosis is similar to the experimental form obtained by Schweizer on the rat. The occurrence of intracellular tyrosine crystals probably damages lysosine membrane and the release lysomie proteases induce the cellular lesions.", "contents": "[Richner-Hanhart's syndrome or oculo-cutaneous tyrosinosis (about one case) (author's transl)]. Richner-Hanhart's syndrome correspond to an hypertyrosinemia due to a deficiency of a soluble tyrosine amino-transferase. This recently described tyrosinosis has been called oculo-cutaneous tyrosinosis. This disease transmitted on a recessive way in amenable to a treatment by a low tyrosine diet. In an infant, 18 months old, presenting a bilateral dendritic keratitis, a punctiform keratosis of the extremities, a patchy leucokeratosis of the tongue and a mental ketardation, the hypertyrosinemia reached 52 mg per 100 ml and the urine demonstrated the presence of phenyl-atonic acids. There was no hepato-renal involvement. The deferency of soluble amino-transferase was studied on the hepatocytes and confirmed. The low tyrosine diet made the clinical and biological signs disappear. The improvement was noticeable from the first week on and continued during the 16 months of the follow-up. There was no ill effect of the special diet on the weight and height growth. The oculo-cutaneous tyrosinosis is similar to the experimental form obtained by Schweizer on the rat. The occurrence of intracellular tyrosine crystals probably damages lysosine membrane and the release lysomie proteases induce the cellular lesions."}
{"id": "PMID:485022", "title": "[Presence of clusters of Langerhans cells and lymphoid cells in the peritumoral infiltrate of a case of basal cell naevus syndrome. Ultrastructural study (author's transl)].", "content": "A case of naevoid basal cell carcinoma syndrome with clusters of Langerhans' and lymphoid cells in the inflammatory peritumoral infiltrate, is reported. Electron microscopy shows intercellular contacts between eight to ten lymphoid cells and a single Langerhans' cell. These contacts occur over limited areas of the cell membranes by means of zones of high electron density on the outer edge of the cytoplasm. The functional significance of this finding is discussed, bearing in mind that recent observations have established certain analogies between Langerhans' and interdigitating reticulum cells. The latter might, within the thymus-dependent region of the lymph nodes, contribute to the formation of a micro-environment favourable to certain immunological activities of T-lymphocyte populations.", "contents": "[Presence of clusters of Langerhans cells and lymphoid cells in the peritumoral infiltrate of a case of basal cell naevus syndrome. Ultrastructural study (author's transl)]. A case of naevoid basal cell carcinoma syndrome with clusters of Langerhans' and lymphoid cells in the inflammatory peritumoral infiltrate, is reported. Electron microscopy shows intercellular contacts between eight to ten lymphoid cells and a single Langerhans' cell. These contacts occur over limited areas of the cell membranes by means of zones of high electron density on the outer edge of the cytoplasm. The functional significance of this finding is discussed, bearing in mind that recent observations have established certain analogies between Langerhans' and interdigitating reticulum cells. The latter might, within the thymus-dependent region of the lymph nodes, contribute to the formation of a micro-environment favourable to certain immunological activities of T-lymphocyte populations."}
{"id": "PMID:485023", "title": "[Median candidiasis of the tongue (glossitis mediana candidamycetica) (author's transl)].", "content": "The median rhomboid glossitis is generally considered as a developmental anomaly of the tongue. Recent clinical, biological and experimental studies provide support for a fongous etiology: this lesion of the midline of the tongue is a chronic hyperplastic oral candidiasis, occurring sometimes in association with candidal commissural leukoplakias and palatine kissing lesions. The yeasts--mainly Candida albicans-- invade the upper parakeratotic layers of the epithelium inducing a proliferating acanthosis of the deeper epithelial ridges; superficial micro-pustules closely related to the candidal hyphae were found in most cases. Antifungal specific therapy appear effective in early lesions; later the condition becomes permanent and, in a few cases, may go further to malignant change. Fourteen cases are presented in this paper: the clinical, histopathological and therapeutic features are obviously in accord with this fongous infectious etiology (already suggested and demonstrated in 1965 by French stomatologists) and with the more recent experimental data published by Cawson, Sohnel and Kirkpatrick, Jones and Russell.", "contents": "[Median candidiasis of the tongue (glossitis mediana candidamycetica) (author's transl)]. The median rhomboid glossitis is generally considered as a developmental anomaly of the tongue. Recent clinical, biological and experimental studies provide support for a fongous etiology: this lesion of the midline of the tongue is a chronic hyperplastic oral candidiasis, occurring sometimes in association with candidal commissural leukoplakias and palatine kissing lesions. The yeasts--mainly Candida albicans-- invade the upper parakeratotic layers of the epithelium inducing a proliferating acanthosis of the deeper epithelial ridges; superficial micro-pustules closely related to the candidal hyphae were found in most cases. Antifungal specific therapy appear effective in early lesions; later the condition becomes permanent and, in a few cases, may go further to malignant change. Fourteen cases are presented in this paper: the clinical, histopathological and therapeutic features are obviously in accord with this fongous infectious etiology (already suggested and demonstrated in 1965 by French stomatologists) and with the more recent experimental data published by Cawson, Sohnel and Kirkpatrick, Jones and Russell."}
{"id": "PMID:485028", "title": "[Incontinentia pigmenti. Study of 7 cases, 2 in men (author's transl)].", "content": "We describe 7 observations of incontinentia pigmenti (0.0026 p. 100 of the patients visited in the Department of Medical-Surgical Dermatology, Faculty of Medicine, University of Granada). Two cases with congenital pigmentation and one of these with secondary inflammatory lesions. The electron microscopy shows relation between the classic stages of the process. In two patients there were defects of the Central Nervous System: brain paralysis and microcephaly. Only two patients with blood eosinophilia (13 p. 100-21 p. 100). Evolution benign, except in the case with brain paralysis. There were familiar antecedents in three women and these were not found in two women and two men with normal karyotype. It suggests a mutation. This study has shown a high rate men/women: 2/5; in comparison to other series. We think that it is possible for a mutation and that this disease is more frequent than it seems. It is necessary to think in this disease in all pigmentary abnormalities in children.", "contents": "[Incontinentia pigmenti. Study of 7 cases, 2 in men (author's transl)]. We describe 7 observations of incontinentia pigmenti (0.0026 p. 100 of the patients visited in the Department of Medical-Surgical Dermatology, Faculty of Medicine, University of Granada). Two cases with congenital pigmentation and one of these with secondary inflammatory lesions. The electron microscopy shows relation between the classic stages of the process. In two patients there were defects of the Central Nervous System: brain paralysis and microcephaly. Only two patients with blood eosinophilia (13 p. 100-21 p. 100). Evolution benign, except in the case with brain paralysis. There were familiar antecedents in three women and these were not found in two women and two men with normal karyotype. It suggests a mutation. This study has shown a high rate men/women: 2/5; in comparison to other series. We think that it is possible for a mutation and that this disease is more frequent than it seems. It is necessary to think in this disease in all pigmentary abnormalities in children."}
{"id": "PMID:485029", "title": "[Eosinophilic pustular folliculitis (Ofuji) (author's transl)].", "content": "A case, the first French observation of eosinophilic pustular folliculitis (Ofuji) is reported. The long lasting condition of unknown etiology is characterized by slightly indurated erythematous plaques and formation of follicular small papules and pustules. The lesions have a tendency to central healing and peripheral extension and proceed with remissions and exacerbations. Histological features are pustular folliculitis composed chiefly of eosinophiles. Blood eosinophila is often founded. Local corticotherapy and dapsone are sometimes a effective treatment.", "contents": "[Eosinophilic pustular folliculitis (Ofuji) (author's transl)]. A case, the first French observation of eosinophilic pustular folliculitis (Ofuji) is reported. The long lasting condition of unknown etiology is characterized by slightly indurated erythematous plaques and formation of follicular small papules and pustules. The lesions have a tendency to central healing and peripheral extension and proceed with remissions and exacerbations. Histological features are pustular folliculitis composed chiefly of eosinophiles. Blood eosinophila is often founded. Local corticotherapy and dapsone are sometimes a effective treatment."}
{"id": "PMID:485030", "title": "[Xanthoma disseminatum (Montgomery's syndrome) (author's transl)].", "content": "Montgomery's syndrome is distinguished by skin and mucous membrane xanthomatosis, predominant on folds, associated with diabetes insipidus and normal lipidic balance. From one typical case history occurring in a young gravida, the authors stresse upon: --Review of literature's cases. --Peculiar therapeutic problems during pregnancy. --Nosological discussion: the position of Montgomery's syndrome compared with histocytosis X and other nonnormolipidic xanthomatosis. This problem is all the more important as in this observation xanthomatosis was first with a normal lipidic measurement, lipidic disorders appearing only secondarily.", "contents": "[Xanthoma disseminatum (Montgomery's syndrome) (author's transl)]. Montgomery's syndrome is distinguished by skin and mucous membrane xanthomatosis, predominant on folds, associated with diabetes insipidus and normal lipidic balance. From one typical case history occurring in a young gravida, the authors stresse upon: --Review of literature's cases. --Peculiar therapeutic problems during pregnancy. --Nosological discussion: the position of Montgomery's syndrome compared with histocytosis X and other nonnormolipidic xanthomatosis. This problem is all the more important as in this observation xanthomatosis was first with a normal lipidic measurement, lipidic disorders appearing only secondarily."}
{"id": "PMID:485035", "title": "[Plasmodium chabaudi: changes in the surface sialic acids of red blood cells during infection (author's transl)].", "content": "Surface sialic acids of mice red blood cells infected with the malarial parasite Plasmodium chabaudi are increased as compared to extracts of red cells from normal mice. This increase occurs coincidentally when reticulocytes increase in the peripheral blood. The same increase of surface sialic acids is shown by studies of reticulocytes from phenylhydrazine treated mice. Similarly, neutral hexoses, fucose, were found in greater quantities in surface glycopeptides obtained after incubation with trypsin of reticulocytes from phenylhydrazine treated mice than in surface glycopeptides of red cells from normal mice. This increase occurs also in Plasmodium chabaudi infected red cells with the development of reticulocytosis, in the same way. These facts suggest the contribution of reticulocytes to the biochemical changes observed during malaria infection.", "contents": "[Plasmodium chabaudi: changes in the surface sialic acids of red blood cells during infection (author's transl)]. Surface sialic acids of mice red blood cells infected with the malarial parasite Plasmodium chabaudi are increased as compared to extracts of red cells from normal mice. This increase occurs coincidentally when reticulocytes increase in the peripheral blood. The same increase of surface sialic acids is shown by studies of reticulocytes from phenylhydrazine treated mice. Similarly, neutral hexoses, fucose, were found in greater quantities in surface glycopeptides obtained after incubation with trypsin of reticulocytes from phenylhydrazine treated mice than in surface glycopeptides of red cells from normal mice. This increase occurs also in Plasmodium chabaudi infected red cells with the development of reticulocytosis, in the same way. These facts suggest the contribution of reticulocytes to the biochemical changes observed during malaria infection."}
{"id": "PMID:485036", "title": "[Leishmaniasis in Greece. Results of an entomological survey during June 1977 (author's transl)].", "content": "In relation with the recent outbreak of leishmaniasis in Greece, the authors undertook entomological investigations for delimiting the foci of transmission. A first survey, during june 1977, in continental Greece, gave 6 633 sandflies with 21% of P. major and 20% of P. perfiliewi. Captures are analysed for each species with systematic and epidemiological comments.", "contents": "[Leishmaniasis in Greece. Results of an entomological survey during June 1977 (author's transl)]. In relation with the recent outbreak of leishmaniasis in Greece, the authors undertook entomological investigations for delimiting the foci of transmission. A first survey, during june 1977, in continental Greece, gave 6 633 sandflies with 21% of P. major and 20% of P. perfiliewi. Captures are analysed for each species with systematic and epidemiological comments."}
{"id": "PMID:485039", "title": "[Contribution to the study of Microphallidae Travassos, 1920 (Trematoda). XXXIV. Redescription of Levinseniella howensis Johnston, 1917 (author's transl)].", "content": "According to the type of L. howensis the species belongs to the subgenus Levinseniella instead of the subgenus Monarrhenos as believed before. The genital atrium has two male pockets and one female pouch.", "contents": "[Contribution to the study of Microphallidae Travassos, 1920 (Trematoda). XXXIV. Redescription of Levinseniella howensis Johnston, 1917 (author's transl)]. According to the type of L. howensis the species belongs to the subgenus Levinseniella instead of the subgenus Monarrhenos as believed before. The genital atrium has two male pockets and one female pouch."}
{"id": "PMID:485045", "title": "Bradshaw lecture, 1978. Recent trends in the practice of sphincter-saving excision for rectal cancer.", "content": "Current fashions in the use of sphincter-saving resections for carcinoma of the rectum are surveyed, with particular reference to the management of growths of the middle third and to the potential role of recently introduced intestinal stapling devices for construction of the anastomoses.", "contents": "Bradshaw lecture, 1978. Recent trends in the practice of sphincter-saving excision for rectal cancer. Current fashions in the use of sphincter-saving resections for carcinoma of the rectum are surveyed, with particular reference to the management of growths of the middle third and to the potential role of recently introduced intestinal stapling devices for construction of the anastomoses."}
{"id": "PMID:485046", "title": "Surgical treatment of bronchiectasis.", "content": "In recent years several reports have suggested that pulmonary resection is the treatment of choice in selected cases of primary bronchiectasis. In this study the clinical features and results of surgical treatment in 53 cases of primary bronchiectasis are described.", "contents": "Surgical treatment of bronchiectasis. In recent years several reports have suggested that pulmonary resection is the treatment of choice in selected cases of primary bronchiectasis. In this study the clinical features and results of surgical treatment in 53 cases of primary bronchiectasis are described."}
{"id": "PMID:485040", "title": "[About Serpentostephanus Sudarikov, 1961 (Strigeata : Cyathocotyloidea : Prohemistomidae : Szidatinae) (author's transl)].", "content": "The genus Serpentostephanus Sudarikov, 1961 is placed in the subfamily Szydatinae Dubois, 1938, the diagnosis of which is emended. It is near to being Gogatea Lutz, 1935, Szidatia Dubois, 1938 et Mesostephanoides Dubois, 1951. A key of the genera is proposed.", "contents": "[About Serpentostephanus Sudarikov, 1961 (Strigeata : Cyathocotyloidea : Prohemistomidae : Szidatinae) (author's transl)]. The genus Serpentostephanus Sudarikov, 1961 is placed in the subfamily Szydatinae Dubois, 1938, the diagnosis of which is emended. It is near to being Gogatea Lutz, 1935, Szidatia Dubois, 1938 et Mesostephanoides Dubois, 1951. A key of the genera is proposed."}
{"id": "PMID:485041", "title": "[Intestinal schistosomiasis in the freshwater mangrove of Guadalupe (French Antilles). Ecology of the vector, Biomphalaria glabrata and its parasite Schistosoma mansoni (author's transl)].", "content": "In the freshwater mangrove of Dubelloy-Devarieux (Guadalupe), the dynamics of populations of B. glabrata and the transmission of S. mansoni, mostly depends on the alternation of dry and rainy season. The flooding of the mangrove (in september), permits a yearly renewal of the malacological populations. The sizes analysis of the snails shows that the life cycle of B. glabrata include two rainy seasons separated by one dry season. The prevalence of B. glabrata (0,25%) and cercariae densities (0,8 c/l) are very slow. The acrophase of the rhythm of presence in standing waters is reached at 2 pm. Men and rats are infected. The infections of rats is of 20% at the beginning of the rainy season and of 100% at the end of the rainy season. Principal factors involved in the infection of rats are discussed.", "contents": "[Intestinal schistosomiasis in the freshwater mangrove of Guadalupe (French Antilles). Ecology of the vector, Biomphalaria glabrata and its parasite Schistosoma mansoni (author's transl)]. In the freshwater mangrove of Dubelloy-Devarieux (Guadalupe), the dynamics of populations of B. glabrata and the transmission of S. mansoni, mostly depends on the alternation of dry and rainy season. The flooding of the mangrove (in september), permits a yearly renewal of the malacological populations. The sizes analysis of the snails shows that the life cycle of B. glabrata include two rainy seasons separated by one dry season. The prevalence of B. glabrata (0,25%) and cercariae densities (0,8 c/l) are very slow. The acrophase of the rhythm of presence in standing waters is reached at 2 pm. Men and rats are infected. The infections of rats is of 20% at the beginning of the rainy season and of 100% at the end of the rainy season. Principal factors involved in the infection of rats are discussed."}
{"id": "PMID:485047", "title": "The surgical anatomy of the superficial and perforating veins of the lower limb.", "content": "The superficial venous system was dissected in 60 legs. A possible function is ascribed to the long saphenous vein and an argument against its stripping in varicose veins presented. Abnormality of the long saphenous vein and its association with varicose veins is described. Particular features of the saphenous tributaries are noted and their relevance in varicosity discussed. The perforator system is described, the distinguishing properties of the medial ankle perforators being examined and their anatomical confinement delineated.", "contents": "The surgical anatomy of the superficial and perforating veins of the lower limb. The superficial venous system was dissected in 60 legs. A possible function is ascribed to the long saphenous vein and an argument against its stripping in varicose veins presented. Abnormality of the long saphenous vein and its association with varicose veins is described. Particular features of the saphenous tributaries are noted and their relevance in varicosity discussed. The perforator system is described, the distinguishing properties of the medial ankle perforators being examined and their anatomical confinement delineated."}
{"id": "PMID:485042", "title": "Helminths of birds and mammals of Israel. VIII.- Skrjabinocapillaria rodentium n. sp. (Nematoda Capillariidae) from gerbillid and murid rodents.", "content": "Skrjabinocapillaria rodentium n.sp., found in the stomach of Meriones crassus, Gerbillus pyramidum, G. gerbillus, G. dasyurus and Acomys cahirinus, is described. It differs from the other two species in the genus, S. bakeri and S. eubursata, mainly in being larger, having a larger number of stichocytes and having a cephalic influation in the female and not in the male.", "contents": "Helminths of birds and mammals of Israel. VIII.- Skrjabinocapillaria rodentium n. sp. (Nematoda Capillariidae) from gerbillid and murid rodents. Skrjabinocapillaria rodentium n.sp., found in the stomach of Meriones crassus, Gerbillus pyramidum, G. gerbillus, G. dasyurus and Acomys cahirinus, is described. It differs from the other two species in the genus, S. bakeri and S. eubursata, mainly in being larger, having a larger number of stichocytes and having a cephalic influation in the female and not in the male."}
{"id": "PMID:485048", "title": "An open technique of pleural biopsy in the diagnosis of tuberculous effusions.", "content": "To assess an open technique of pleural biopsy as an aid to diagnosis in pleural disease 107 African patients with radiological evidence of pleural effusion underwent biopsy. In 87 there was radiological evidence of an effusion but not of underlying lung disease; 73 patients (84%) in this group were ultimately diagnosed as suffering from tuberculosis and of these 56 (77%) had a positive pleural biopsy. There was a heavy male predominance of tuberculous infection (male:female ratio approximately 5:1) and half of the patients were aged 21 to 30 years. In the 20 patients with radiological changes in the lung a diagnosis was established by biopsy in 13 cases. Four of these were tuberculous and a further two cases of tuberculosis were established on clinical grounds.", "contents": "An open technique of pleural biopsy in the diagnosis of tuberculous effusions. To assess an open technique of pleural biopsy as an aid to diagnosis in pleural disease 107 African patients with radiological evidence of pleural effusion underwent biopsy. In 87 there was radiological evidence of an effusion but not of underlying lung disease; 73 patients (84%) in this group were ultimately diagnosed as suffering from tuberculosis and of these 56 (77%) had a positive pleural biopsy. There was a heavy male predominance of tuberculous infection (male:female ratio approximately 5:1) and half of the patients were aged 21 to 30 years. In the 20 patients with radiological changes in the lung a diagnosis was established by biopsy in 13 cases. Four of these were tuberculous and a further two cases of tuberculosis were established on clinical grounds."}
{"id": "PMID:485043", "title": "[Redescription of Onchocerca gibsoni C. et J., 1910 (author's transl)].", "content": "O. gibsoni is redescribed; the hypoderma and the musculature of the female body are studied on transversal sections and compared to several other species of Onchocerca. These structures and the general morphology show the autonomy of a small line of asiatic and african Onchocerca of Bovines (O. gibsoni, O. dukei, O. ochengi and, probably, the other nodular Onchocerca of Bovinae), to which the human parasite, O. volvulus, belongs. The presence of O. gibsoni in Africa is doubtful.", "contents": "[Redescription of Onchocerca gibsoni C. et J., 1910 (author's transl)]. O. gibsoni is redescribed; the hypoderma and the musculature of the female body are studied on transversal sections and compared to several other species of Onchocerca. These structures and the general morphology show the autonomy of a small line of asiatic and african Onchocerca of Bovines (O. gibsoni, O. dukei, O. ochengi and, probably, the other nodular Onchocerca of Bovinae), to which the human parasite, O. volvulus, belongs. The presence of O. gibsoni in Africa is doubtful."}
{"id": "PMID:485044", "title": "[Baeodasymyia modesta n. g., n. sp. from the Republic of Ha\u00efti (Diptera, Ceratopogonidae) (author's transl)].", "content": "The authors describe a new genus and a new species of Ceratopogonidae: Baeodasymyia modesta n.g., n.sp., which somewhat resemble the two genera Paradasyhelea Macfie, 1940 and Baeohelea Wirth and Blanton, 1970.", "contents": "[Baeodasymyia modesta n. g., n. sp. from the Republic of Ha\u00efti (Diptera, Ceratopogonidae) (author's transl)]. The authors describe a new genus and a new species of Ceratopogonidae: Baeodasymyia modesta n.g., n.sp., which somewhat resemble the two genera Paradasyhelea Macfie, 1940 and Baeohelea Wirth and Blanton, 1970."}
{"id": "PMID:485089", "title": "Induction of urogenital neoplasia and abnormalities from prenatal exposure to diethylstilbestrol.", "content": "The occurrence of vaginal clear cell adenocarcinoma in young women following exposure in utero to diethylstilbestrol (DES) is now well documented. In addition to this carcinogenic potential. DES has been shown to be teratogenic. In females, the DES-related malformations include vaginal adenosis, transverse ridges of the vagina or cervix and uterine abnormalities. Although no neoplasms have been observed in DES-exposed males, malformations of the epididymis, testes and phallus are relatively common and may result in infertility. The carcinogenic mechanism of DES may be either a direct induction of malignant potential in vaginal cells or a teratogenic effect causing ectopic M\u00fcllerian epithelium which could be exposed later to mutagenic agents in the vagina. The absence of malignancy in DES-exposed males may favor the latter hypothesis since male M\u00fcllerian remnants are internal structures and thus would not be exposed to surface carcinogens.", "contents": "Induction of urogenital neoplasia and abnormalities from prenatal exposure to diethylstilbestrol. The occurrence of vaginal clear cell adenocarcinoma in young women following exposure in utero to diethylstilbestrol (DES) is now well documented. In addition to this carcinogenic potential. DES has been shown to be teratogenic. In females, the DES-related malformations include vaginal adenosis, transverse ridges of the vagina or cervix and uterine abnormalities. Although no neoplasms have been observed in DES-exposed males, malformations of the epididymis, testes and phallus are relatively common and may result in infertility. The carcinogenic mechanism of DES may be either a direct induction of malignant potential in vaginal cells or a teratogenic effect causing ectopic M\u00fcllerian epithelium which could be exposed later to mutagenic agents in the vagina. The absence of malignancy in DES-exposed males may favor the latter hypothesis since male M\u00fcllerian remnants are internal structures and thus would not be exposed to surface carcinogens."}
{"id": "PMID:485090", "title": "Ectopic hormone production by malignant tumors.", "content": "Malignant tumours of nonendocrine tissues may produce ectopic hormones. The most likely mechanism is depression of genes which code for hormones. Ectopic hormones are invariably peptides, and each is identical to some peptide product of an endocrine gland. However, the majority of ectopic hormones occur as biologically inactive precursors or subunits and therefore remain occult unless they are specifically sought. When appropriate assays are made for such inactive forms, it is found that ectopic production of hormone-like peptides occurs frequently. Clinical syndromes result only in the relatively rare patients in whom a biologically active form is synthesized in large quantities. Laboratory research in this area improves our understanding of genetic control mechanisms in neoplasia. Ectopic hormones may be of limited use in diagnosis of cancer, especially when multiple markers are measured simultaneously.", "contents": "Ectopic hormone production by malignant tumors. Malignant tumours of nonendocrine tissues may produce ectopic hormones. The most likely mechanism is depression of genes which code for hormones. Ectopic hormones are invariably peptides, and each is identical to some peptide product of an endocrine gland. However, the majority of ectopic hormones occur as biologically inactive precursors or subunits and therefore remain occult unless they are specifically sought. When appropriate assays are made for such inactive forms, it is found that ectopic production of hormone-like peptides occurs frequently. Clinical syndromes result only in the relatively rare patients in whom a biologically active form is synthesized in large quantities. Laboratory research in this area improves our understanding of genetic control mechanisms in neoplasia. Ectopic hormones may be of limited use in diagnosis of cancer, especially when multiple markers are measured simultaneously."}
{"id": "PMID:485097", "title": "[Transferrin types in the Dama (Southwest Africa)].", "content": "The transferrin type of 238 Dama serums was determined and an estimate made of the gene frequencies. Using the SANGHVI method, the distribution of the transferrin types of the Dama was compared with 53 African populations south of the Sahara. The comparison demonstrates the great dissimilarity of the Dama to the Khoisanide tribes and confirms--at least in part--the results of polysymptomatic affinity comparisons of the Dama with other African populations.", "contents": "[Transferrin types in the Dama (Southwest Africa)]. The transferrin type of 238 Dama serums was determined and an estimate made of the gene frequencies. Using the SANGHVI method, the distribution of the transferrin types of the Dama was compared with 53 African populations south of the Sahara. The comparison demonstrates the great dissimilarity of the Dama to the Khoisanide tribes and confirms--at least in part--the results of polysymptomatic affinity comparisons of the Dama with other African populations."}
{"id": "PMID:485091", "title": "Electron microscopy in the diagnosis of pituitary tumors.", "content": "The usefulness and limitations of electron microscopy (EM) in pituitary tumor diagnosis are reviewed and illustrated with clinical examples. The traditional classification of chromophil and chromophobe adenomas is often inconsistent with the hormonal activity of the tumors. Virtually all pituitary adenomas contain some secretory granules when viewed with EM. Endocrine inactive chromophobe adenomas contain 150 nm granules with no demonstrable hormone function. Typical growth hormone (GH) secreting eosinophil adenomas contain large 375 nm granules which dominate the cell cytoplasm. GH secreting chromophobic tumors contain secretory granules of abnormal size and concentration which are invisible to the light microscopist. The variability in granule size may indicate the production of abnormal granules or reflect the stage of the cell in a secretory cycle. Because of this wide range in granule size, the identification of tumor cell type or hormone produced is not reliable by granule measurement alone. Some neoplasms in the sella turcica may be so bizzare or undifferentiated as to defy classification. In such instances, EM can reveal ultrastructural details which identify their origin from pituitary tissue. Malignant pituitary tumors may contain minute secretory granules, and rare pituitary oncocytomas are packed with abnormal mitochondria.", "contents": "Electron microscopy in the diagnosis of pituitary tumors. The usefulness and limitations of electron microscopy (EM) in pituitary tumor diagnosis are reviewed and illustrated with clinical examples. The traditional classification of chromophil and chromophobe adenomas is often inconsistent with the hormonal activity of the tumors. Virtually all pituitary adenomas contain some secretory granules when viewed with EM. Endocrine inactive chromophobe adenomas contain 150 nm granules with no demonstrable hormone function. Typical growth hormone (GH) secreting eosinophil adenomas contain large 375 nm granules which dominate the cell cytoplasm. GH secreting chromophobic tumors contain secretory granules of abnormal size and concentration which are invisible to the light microscopist. The variability in granule size may indicate the production of abnormal granules or reflect the stage of the cell in a secretory cycle. Because of this wide range in granule size, the identification of tumor cell type or hormone produced is not reliable by granule measurement alone. Some neoplasms in the sella turcica may be so bizzare or undifferentiated as to defy classification. In such instances, EM can reveal ultrastructural details which identify their origin from pituitary tissue. Malignant pituitary tumors may contain minute secretory granules, and rare pituitary oncocytomas are packed with abnormal mitochondria."}
{"id": "PMID:485098", "title": "[Iris heterochromia: variations in form, age changes, sex dimorphism].", "content": "On a sample of n = 25,346 individuals from Vienna (10,855 males, 14,491 females) the iris pigmentation has been typed by the author. In this sample 65 cases of different types of heterochromia were found. The frequency of the occurrence of the different types of this anomaly could be calculated for the first time. Neglecting age, sex, and type of heterochromia the total frequency amounts to 0.256 (males: 0.157; females: 0.37) %. The variants of heterochromia very from cases of total heterochromia to those of various size; in addition to it a number of special types could be observed. The localization of the heterochrome parts of the iris shows certain variations; mostly, however, it is seen in the lower half of the iris. The nasal part is concerned in only one case; the temporal region never. Thus, the partial heterochromia is characteristic by a marked dependency of its localization. The colour of heterochromia varies from 2 a-14 according to the Martin-Schultz standard set of coloured eyes. However, No. 9 (ca. 50%) and No. 7 (ca. 20%) were the most frequent colours. The age variations are considerable. 5/6 of all heterochromias were found between the age from 2-19 years. Finally, a marked sexual dimorphism was observed, as in females heterochromia is much more frequent than in males.", "contents": "[Iris heterochromia: variations in form, age changes, sex dimorphism]. On a sample of n = 25,346 individuals from Vienna (10,855 males, 14,491 females) the iris pigmentation has been typed by the author. In this sample 65 cases of different types of heterochromia were found. The frequency of the occurrence of the different types of this anomaly could be calculated for the first time. Neglecting age, sex, and type of heterochromia the total frequency amounts to 0.256 (males: 0.157; females: 0.37) %. The variants of heterochromia very from cases of total heterochromia to those of various size; in addition to it a number of special types could be observed. The localization of the heterochrome parts of the iris shows certain variations; mostly, however, it is seen in the lower half of the iris. The nasal part is concerned in only one case; the temporal region never. Thus, the partial heterochromia is characteristic by a marked dependency of its localization. The colour of heterochromia varies from 2 a-14 according to the Martin-Schultz standard set of coloured eyes. However, No. 9 (ca. 50%) and No. 7 (ca. 20%) were the most frequent colours. The age variations are considerable. 5/6 of all heterochromias were found between the age from 2-19 years. Finally, a marked sexual dimorphism was observed, as in females heterochromia is much more frequent than in males."}
{"id": "PMID:485099", "title": "Incidence of squatting facets on the talus of Indians (Agra region).", "content": "230 adult Indian tali (from Agra region) were studied for the incidence of squatting facets. Extension of medial articular facet was observed in all the cases. Medial extension of trochlea was observed in 37% of cases; lateral extension of trochlea in 71.6% of cases, lateral squatting facets in 43.5% of cases and medial squatting in 8.6% of cases. The present data are compared with that of other workers.", "contents": "Incidence of squatting facets on the talus of Indians (Agra region). 230 adult Indian tali (from Agra region) were studied for the incidence of squatting facets. Extension of medial articular facet was observed in all the cases. Medial extension of trochlea was observed in 37% of cases; lateral extension of trochlea in 71.6% of cases, lateral squatting facets in 43.5% of cases and medial squatting in 8.6% of cases. The present data are compared with that of other workers."}
{"id": "PMID:485094", "title": "Thin needle aspiration biopsy of endocrine organs.", "content": "The purpose of this paper is to summarize the advantages and disadvantages of the fine needle aspiration technique in reference to the endocrine organs. The principles of technique and interpretation are presented. The application of aspiration biopsies to the breast, the prostate, the pancreas and the thyroid are briefly discussed.", "contents": "Thin needle aspiration biopsy of endocrine organs. The purpose of this paper is to summarize the advantages and disadvantages of the fine needle aspiration technique in reference to the endocrine organs. The principles of technique and interpretation are presented. The application of aspiration biopsies to the breast, the prostate, the pancreas and the thyroid are briefly discussed."}
{"id": "PMID:485092", "title": "Nickel concentrations in nasal mucosa, plasma, and urine in active and retired nickel workers.", "content": "The objective of the present investigation was to study the influence of the occupational nickel exposure on the concentration of nickel in nasal mucosa, plasma and urine. Plasma, urine and biopsy specimens of nasal mucosa from 318 nickel workers, 15 retired nickel workers and 57 non-exposed controls were analyzed for nickel by atomic absorption spectrometry. The results showed that nickel exposure led significantly to raised nickel concentration in nasal mucosa, plasma and urine both in active and retired nickel workers. The average nickel concentration in the nasal mucosa was highest in workers exposed to the highest atmospheric nickel concentration, inhaled as nickel subsulphide and oxide dust. Workers exposed to aerosols of nickel chloride and sulphate at a lower atmospheric nickel concentration had, on the other hand, the highest mean nickel concentration in plasma and urine. The mucosal, plasma and urine nickel concentration were significantly correlated to duration of nickel exposure. The accumulated nickel in the nasal mucosa was retained for years after termination of the nickel exposure, and slowly released with an estimated half-life of 3.5 years.", "contents": "Nickel concentrations in nasal mucosa, plasma, and urine in active and retired nickel workers. The objective of the present investigation was to study the influence of the occupational nickel exposure on the concentration of nickel in nasal mucosa, plasma and urine. Plasma, urine and biopsy specimens of nasal mucosa from 318 nickel workers, 15 retired nickel workers and 57 non-exposed controls were analyzed for nickel by atomic absorption spectrometry. The results showed that nickel exposure led significantly to raised nickel concentration in nasal mucosa, plasma and urine both in active and retired nickel workers. The average nickel concentration in the nasal mucosa was highest in workers exposed to the highest atmospheric nickel concentration, inhaled as nickel subsulphide and oxide dust. Workers exposed to aerosols of nickel chloride and sulphate at a lower atmospheric nickel concentration had, on the other hand, the highest mean nickel concentration in plasma and urine. The mucosal, plasma and urine nickel concentration were significantly correlated to duration of nickel exposure. The accumulated nickel in the nasal mucosa was retained for years after termination of the nickel exposure, and slowly released with an estimated half-life of 3.5 years."}
{"id": "PMID:485101", "title": "[Stomatological studies of the slavic cemetery at the Spandau Burgwall, Berlin].", "content": "The dentition of a medieval population from the former Spandau Burgwall in Berlin was investigated with regard to caries and other abnormities of the teeth, the palate and the jaws. The caries frequency amounts to 7.9%, while 63.6% of the individuals had a caries dentition. The diminuation of the caries frequency from the juveniles to the 40--60 years old individuals depends on the high rate of loss of tooth during life. Probably, all teeth which have been lost during life can be evaluated as decayed teeth. X-ray pictures have shown that three sets of teeth have hypercementotic alterations at the roots, eight permanent dentitions have cysts and apical parodontitis. Supernumerary cuspids at the toothcrowns are to be seen as a tuberculum paramolare and a tuberculum intermedium. A second upper molar has a reduced crown with only two cuspids and shows the tendency of reduction to a bicuspid. Toothrotation and other deviations of teeth from their position in the row are frequent in this material. Four deciduous dentitions of children were investigated, too. Three carious teeth were found among 45 available teeth, two deciduous molars and one first upper molar show a Carabelli's tubercle, the other first molar has a fovea carabelli.", "contents": "[Stomatological studies of the slavic cemetery at the Spandau Burgwall, Berlin]. The dentition of a medieval population from the former Spandau Burgwall in Berlin was investigated with regard to caries and other abnormities of the teeth, the palate and the jaws. The caries frequency amounts to 7.9%, while 63.6% of the individuals had a caries dentition. The diminuation of the caries frequency from the juveniles to the 40--60 years old individuals depends on the high rate of loss of tooth during life. Probably, all teeth which have been lost during life can be evaluated as decayed teeth. X-ray pictures have shown that three sets of teeth have hypercementotic alterations at the roots, eight permanent dentitions have cysts and apical parodontitis. Supernumerary cuspids at the toothcrowns are to be seen as a tuberculum paramolare and a tuberculum intermedium. A second upper molar has a reduced crown with only two cuspids and shows the tendency of reduction to a bicuspid. Toothrotation and other deviations of teeth from their position in the row are frequent in this material. Four deciduous dentitions of children were investigated, too. Three carious teeth were found among 45 available teeth, two deciduous molars and one first upper molar show a Carabelli's tubercle, the other first molar has a fovea carabelli."}
{"id": "PMID:485096", "title": "Dopamine-beta-hydroxylase effectors in neuroblastoma.", "content": "Investigation of dopamine-beta-hydroxylase (DBH) serum activity as a potential diagnostic/prognostic tool for neuroblastoma did not confirm previous reports. However, age-dependent differences in normal children's DBH \"activation\" by water dilution +/- copper addition was revealed. These findings of age-related DBH effector differences suggest that the strong age relationship of DBH activity may be due to alterations in effectors. Additionally, these effectors may be variably affected by assay methodology, influencing DBH results obtained in neuroblastoma and other disease states.", "contents": "Dopamine-beta-hydroxylase effectors in neuroblastoma. Investigation of dopamine-beta-hydroxylase (DBH) serum activity as a potential diagnostic/prognostic tool for neuroblastoma did not confirm previous reports. However, age-dependent differences in normal children's DBH \"activation\" by water dilution +/- copper addition was revealed. These findings of age-related DBH effector differences suggest that the strong age relationship of DBH activity may be due to alterations in effectors. Additionally, these effectors may be variably affected by assay methodology, influencing DBH results obtained in neuroblastoma and other disease states."}
{"id": "PMID:485102", "title": "[Plantar zygodactylous triradii in a population comparison].", "content": "The plantar prints of 125 male Japanese were examined with regard to the frequencies of zygodactylous triradii and their expression types. Comparing the results with those of 500 males Viennese the two samples proved to be significantly different. The differences amount to such an extent that even by enlarging the Japanese sample no fundamental changes in the relations between the two populations might be expected. In Japanese the medium expression occurs in very low frequencies and the strong one not at all, while Europeans show those traits in about 60%. Comparing the pattern types characteristic for zygodactylous triradii, the Japanese revealed almost no proximal loops in the second and almost no whorls in the third interdigital area whereas Viennese showed high frequencies of both types. The findings of a sample of Angolan Bantus as well as those of a German one differ from the Japanese in the same way as the Viennese. It was observed that the line connecting the distal ends of the 2nd, 3rd, 4th and 5th toes is formed rather transversally in Japanese, while it is declining towards the 5th toe in Europeans and in Bantus. It requires further studies to prove if there exists a relation between this anatomical trait and the occurrence of zygodactylous triradii.", "contents": "[Plantar zygodactylous triradii in a population comparison]. The plantar prints of 125 male Japanese were examined with regard to the frequencies of zygodactylous triradii and their expression types. Comparing the results with those of 500 males Viennese the two samples proved to be significantly different. The differences amount to such an extent that even by enlarging the Japanese sample no fundamental changes in the relations between the two populations might be expected. In Japanese the medium expression occurs in very low frequencies and the strong one not at all, while Europeans show those traits in about 60%. Comparing the pattern types characteristic for zygodactylous triradii, the Japanese revealed almost no proximal loops in the second and almost no whorls in the third interdigital area whereas Viennese showed high frequencies of both types. The findings of a sample of Angolan Bantus as well as those of a German one differ from the Japanese in the same way as the Viennese. It was observed that the line connecting the distal ends of the 2nd, 3rd, 4th and 5th toes is formed rather transversally in Japanese, while it is declining towards the 5th toe in Europeans and in Bantus. It requires further studies to prove if there exists a relation between this anatomical trait and the occurrence of zygodactylous triradii."}
{"id": "PMID:485103", "title": "[Formation of the antibiotic complex 4041 by a culture of Actinoplanes ianthinogenes subsp. octamycini subsp. nov].", "content": "A new variety of Actinoplanes, named Actinoplanes ianthinogenes subsp. octamycini Gause et Svechnikova is described. It produces an antibiotic complex consisting of purpuromycin and a new antibiotic belonging to the group of polyens, i. e. octamycin. Maximum accumulation of the above antibiotics in the mycelium was observed in the soybean-glycerol medium.", "contents": "[Formation of the antibiotic complex 4041 by a culture of Actinoplanes ianthinogenes subsp. octamycini subsp. nov]. A new variety of Actinoplanes, named Actinoplanes ianthinogenes subsp. octamycini Gause et Svechnikova is described. It produces an antibiotic complex consisting of purpuromycin and a new antibiotic belonging to the group of polyens, i. e. octamycin. Maximum accumulation of the above antibiotics in the mycelium was observed in the soybean-glycerol medium."}
{"id": "PMID:485104", "title": "[Methodological approaches to developing a method of regulated antibiotic biosynthesis].", "content": "Methodologic approaches to the development of methods for controlled biosynthesis of antibiotics are discussed with reference to oxytetracycline biosynthesis. The method of acute experiments in conjunction with mathematical methods of experiment planning was used for determination of the substrate concentrations optimal for the culture growth and biosynthesis of oxytetracycline. It was found that such mathematic procedure provided simultaneous determination of optimal concentrations of all the substrates.", "contents": "[Methodological approaches to developing a method of regulated antibiotic biosynthesis]. Methodologic approaches to the development of methods for controlled biosynthesis of antibiotics are discussed with reference to oxytetracycline biosynthesis. The method of acute experiments in conjunction with mathematical methods of experiment planning was used for determination of the substrate concentrations optimal for the culture growth and biosynthesis of oxytetracycline. It was found that such mathematic procedure provided simultaneous determination of optimal concentrations of all the substrates."}
{"id": "PMID:485095", "title": "The significance of creatine kinase (CKBB) in metastatic cancer of the prostate.", "content": "Alterations of serum creatine kinase isoenzymes were observed in five cases of prostatic carcinoma. Creatine kinase isoenzyme BB was found in the serum of two of three cases with metastases. Its presence in serum does not seem to be related to acid phosphatase activity but seems associated with extension of the tumor to other tissues. Preliminary studies on effusions from patients with malignant and non-malignant prostates showed that CK-BB was detectable only in cytology positive effusions. This finding suggests that CK-BB may be a tumor product rather than a result of a host response. The observation of CK-BB in a significant percentage of patients (two of three) with metastatic carcinoma of the prostate is of interest and suggests that CK-BB isoenzymes may have some predictive value in following patients with malignant disease.", "contents": "The significance of creatine kinase (CKBB) in metastatic cancer of the prostate. Alterations of serum creatine kinase isoenzymes were observed in five cases of prostatic carcinoma. Creatine kinase isoenzyme BB was found in the serum of two of three cases with metastases. Its presence in serum does not seem to be related to acid phosphatase activity but seems associated with extension of the tumor to other tissues. Preliminary studies on effusions from patients with malignant and non-malignant prostates showed that CK-BB was detectable only in cytology positive effusions. This finding suggests that CK-BB may be a tumor product rather than a result of a host response. The observation of CK-BB in a significant percentage of patients (two of three) with metastatic carcinoma of the prostate is of interest and suggests that CK-BB isoenzymes may have some predictive value in following patients with malignant disease."}
{"id": "PMID:485093", "title": "The microscopical pathology of primary parathyroid hyperplasia.", "content": "The microscopical pathology of the two types of primary parathyroid hyperplasia is reviewed. The importance of the differential diagnosis between chief cell hyperplasia and adenoma is stressed. The problems of the surgical pathologist in masking this differential diagnosis of a biopsy, especially on a frozen section sample, are discussed.", "contents": "The microscopical pathology of primary parathyroid hyperplasia. The microscopical pathology of the two types of primary parathyroid hyperplasia is reviewed. The importance of the differential diagnosis between chief cell hyperplasia and adenoma is stressed. The problems of the surgical pathologist in masking this differential diagnosis of a biopsy, especially on a frozen section sample, are discussed."}
{"id": "PMID:485106", "title": "[Pharmacokinetics and biotransformation of rifamycins in the body of experimental animals].", "content": "The study on distribution of 14C-rifampicin and 14C-rifamycin S in experimental animals after intramuscular administration of the drugs showed that concentrations of rifampicin in the organs and blood were higher than those of rifamycin S. Biotransformation products of both antibiotics, such as 25-deacetylrifampicin, N-oxide of rifampicin, 3-phormylrifamycin SV, rifamycin SV and others were found in the liver, kidneys, bile and urine. No products of the antibiotic metabolism were found in the blood, lungs and spleen.", "contents": "[Pharmacokinetics and biotransformation of rifamycins in the body of experimental animals]. The study on distribution of 14C-rifampicin and 14C-rifamycin S in experimental animals after intramuscular administration of the drugs showed that concentrations of rifampicin in the organs and blood were higher than those of rifamycin S. Biotransformation products of both antibiotics, such as 25-deacetylrifampicin, N-oxide of rifampicin, 3-phormylrifamycin SV, rifamycin SV and others were found in the liver, kidneys, bile and urine. No products of the antibiotic metabolism were found in the blood, lungs and spleen."}
{"id": "PMID:485107", "title": "[Action of penicillin on the causative agent of paratrachoma in the dynamics of an in vitro infection].", "content": "Monolayer cultures of L-cells (mouse fibroblasts) were inoculated with the causative agent of paratrachoma (strain LB-I). Simultaneously penicillin in doses of 0.01, 0.1, 1, 10, 25, 50, 100, 150 and 200 microgram/ml was added and its effect on the causative agent in the infection dynamics (18, 24, 48 and 72 hours after the inoculation) was studied with the light and electron microscopes. Gradual changes in the ultrastructure of the vegetative forms were observed by the 24th hour with the use of penicillin in a dose of 0.01 microgram/ml: the size of the vegetative forms increased, the cell wall membranes separated and periplasmic space significantly enlarged, the protoplast fragments disjoined into it, large forms vacuolized and were fragment with membranes, sometimes multilayer ones. When the culture was exposed to large doses of penicillin, the rate of the changes in the structure was higher and they were simultaneously of several types. Various types of the changes and possible modes of their formation were analyzed. Morphologically they are similar to the processes observed in L-transformation of bacteria. However, these structures were not infectious.", "contents": "[Action of penicillin on the causative agent of paratrachoma in the dynamics of an in vitro infection]. Monolayer cultures of L-cells (mouse fibroblasts) were inoculated with the causative agent of paratrachoma (strain LB-I). Simultaneously penicillin in doses of 0.01, 0.1, 1, 10, 25, 50, 100, 150 and 200 microgram/ml was added and its effect on the causative agent in the infection dynamics (18, 24, 48 and 72 hours after the inoculation) was studied with the light and electron microscopes. Gradual changes in the ultrastructure of the vegetative forms were observed by the 24th hour with the use of penicillin in a dose of 0.01 microgram/ml: the size of the vegetative forms increased, the cell wall membranes separated and periplasmic space significantly enlarged, the protoplast fragments disjoined into it, large forms vacuolized and were fragment with membranes, sometimes multilayer ones. When the culture was exposed to large doses of penicillin, the rate of the changes in the structure was higher and they were simultaneously of several types. Various types of the changes and possible modes of their formation were analyzed. Morphologically they are similar to the processes observed in L-transformation of bacteria. However, these structures were not infectious."}
{"id": "PMID:485108", "title": "[Comparative evaluation of methacycline hydrochloride capsules and tablets according to their dissolubility and rate of solution].", "content": "Desintegration and dissolution of capsules and tablets of methacycline hydrochloride were studied. The study on solubility of methacycline hydrochliride capsules filled with methacycline granulate or powder according to the same formula showed that the rate of the antibiotic liberation from the capsules filled with the powder decreased during storage while that from the capsules filled with the granulate did not change. Investigation of the effect of the mass packing value in a drop on the antibiotic liberation from the capsules showed that an increase in the packing coefficient above 1.38 resulted in a marked decrease in the rate of methacycline liberation from the capsules filled with the granulate. No correlation between desintegration and dissolution of methacycline capsules and tablets was found.", "contents": "[Comparative evaluation of methacycline hydrochloride capsules and tablets according to their dissolubility and rate of solution]. Desintegration and dissolution of capsules and tablets of methacycline hydrochloride were studied. The study on solubility of methacycline hydrochliride capsules filled with methacycline granulate or powder according to the same formula showed that the rate of the antibiotic liberation from the capsules filled with the powder decreased during storage while that from the capsules filled with the granulate did not change. Investigation of the effect of the mass packing value in a drop on the antibiotic liberation from the capsules showed that an increase in the packing coefficient above 1.38 resulted in a marked decrease in the rate of methacycline liberation from the capsules filled with the granulate. No correlation between desintegration and dissolution of methacycline capsules and tablets was found."}
{"id": "PMID:485109", "title": "[Bioavailability of ampicillin suspensions].", "content": "The rate of ampicillin transfer into solution from suspensions of 2 types, i.e. suspensions of anhydrous ampicillin and suspensions of ampicillin trihydrate was studied. Conditions for estimation of the rate of ampicillin dissolution from the suspensions were developed. The pharmacokinetics of the pharmaceutical forms was studied on dogs. Definite advantages of the anhydrous ampicillin suspension were shown as compared to the ampicillin trihydrate suspension.", "contents": "[Bioavailability of ampicillin suspensions]. The rate of ampicillin transfer into solution from suspensions of 2 types, i.e. suspensions of anhydrous ampicillin and suspensions of ampicillin trihydrate was studied. Conditions for estimation of the rate of ampicillin dissolution from the suspensions were developed. The pharmacokinetics of the pharmaceutical forms was studied on dogs. Definite advantages of the anhydrous ampicillin suspension were shown as compared to the ampicillin trihydrate suspension."}
{"id": "PMID:485110", "title": "[Bioavailability of peroral drug forms of oxacillin].", "content": "Bioavailability of experimental tablets and capsules of oxacillin was studied. Significant differences in absorption of the antibiotic from the above dosage forms were found. By the amount of the antibiotic absorbed the capsules were 3 times more effective than the tablets containing the same amount of oxacillin. The therapeutic concentrations of oxacillin in the blood after the use of the capsules persisted by an hour longer than after the use of the tablets. The results of the study on oxacillin absorption in rabbits allowed a prediction of the antibiotic absorption levels in humans.", "contents": "[Bioavailability of peroral drug forms of oxacillin]. Bioavailability of experimental tablets and capsules of oxacillin was studied. Significant differences in absorption of the antibiotic from the above dosage forms were found. By the amount of the antibiotic absorbed the capsules were 3 times more effective than the tablets containing the same amount of oxacillin. The therapeutic concentrations of oxacillin in the blood after the use of the capsules persisted by an hour longer than after the use of the tablets. The results of the study on oxacillin absorption in rabbits allowed a prediction of the antibiotic absorption levels in humans."}
{"id": "PMID:485111", "title": "[Effect of the polyene antibiotic, roseofungin, on the liberation of the intracellular components from Candida guilliermondii cells and from rat erythrocytes].", "content": "Liberation of inorganic ions (potassium and phosphorus), nucleotides and protein from the cells of Candida guilliermondii and rat erythrocytes was studied. It was found that leakage of these components depended on the incubation time, antibiotic dose and amount of the cell material. The potassium ions proved to be most sensitive to the antibiotic: even at low concentrations of roseofungin (2-4 microgram/ml) the amount of the intracellular potassium changed. A decrease in the dry mass and volume concentration in the cells of Candida guilliermondii and lysis in the red blood cells were observed in addition to the leakage of the above intracellular components. The cells of Candida guiliermondii at the beginning of the semilogarithmic growth phase were especially sensitive to roseofungin. The coefficient of roseofungin distribution in the cells of Candida guiliermond\u00fc was evaluated. The maximum distribution coefficient was observed at roseofungin concentrations of 40 and 80 microgram/ml for the 4- and 18-hour cultures respectively. Therefore, the effect of roseofungin is characteristic of polyens.", "contents": "[Effect of the polyene antibiotic, roseofungin, on the liberation of the intracellular components from Candida guilliermondii cells and from rat erythrocytes]. Liberation of inorganic ions (potassium and phosphorus), nucleotides and protein from the cells of Candida guilliermondii and rat erythrocytes was studied. It was found that leakage of these components depended on the incubation time, antibiotic dose and amount of the cell material. The potassium ions proved to be most sensitive to the antibiotic: even at low concentrations of roseofungin (2-4 microgram/ml) the amount of the intracellular potassium changed. A decrease in the dry mass and volume concentration in the cells of Candida guilliermondii and lysis in the red blood cells were observed in addition to the leakage of the above intracellular components. The cells of Candida guiliermondii at the beginning of the semilogarithmic growth phase were especially sensitive to roseofungin. The coefficient of roseofungin distribution in the cells of Candida guiliermond\u00fc was evaluated. The maximum distribution coefficient was observed at roseofungin concentrations of 40 and 80 microgram/ml for the 4- and 18-hour cultures respectively. Therefore, the effect of roseofungin is characteristic of polyens."}
{"id": "PMID:485112", "title": "[Use of carminomycin in combination with UHF hyperthermia in the therapy of sarcoma 180].", "content": "It was found that combined treatment of sarcoma 180 with local U. H. F.-hyperthermia and carminomycin resulted in the tumor growth inhibition by more than 90 per cent, which was much higher than the effect of every agent alone. The thermochemotherapy allowed a decrease in the dose of the antibiotic without decreasing the antitumor effect. The U. H. F.-hyperthermia has an independent tumorolytic effect and promotes selective accumulation of carminomycin by the tumor.", "contents": "[Use of carminomycin in combination with UHF hyperthermia in the therapy of sarcoma 180]. It was found that combined treatment of sarcoma 180 with local U. H. F.-hyperthermia and carminomycin resulted in the tumor growth inhibition by more than 90 per cent, which was much higher than the effect of every agent alone. The thermochemotherapy allowed a decrease in the dose of the antibiotic without decreasing the antitumor effect. The U. H. F.-hyperthermia has an independent tumorolytic effect and promotes selective accumulation of carminomycin by the tumor."}
{"id": "PMID:485113", "title": "[Ampicillin pharmacokinetics in the lymph and blood in acute inflammatory diseases of the abdominal cavity organs].", "content": "Ampicillin levels in the lymph, blood and urine were studied in 15 patients with various inflammatory diseases of the organs of the abdominal cavity. It was found that ampicillin administered intramuscularly to such patients at early postoperative periods was well absorbed not only to the blood but also to the lymph, reaching in parallel its maximum levels in an hour. After that the drug concentration in the lymph persisted for 6 hours and markedly exceeded that in the blood. Adsorption of the lymph lowered the antibiotic concentration in it to 25 per cent indicating the necessity for increasing the drug therapeutic doses.", "contents": "[Ampicillin pharmacokinetics in the lymph and blood in acute inflammatory diseases of the abdominal cavity organs]. Ampicillin levels in the lymph, blood and urine were studied in 15 patients with various inflammatory diseases of the organs of the abdominal cavity. It was found that ampicillin administered intramuscularly to such patients at early postoperative periods was well absorbed not only to the blood but also to the lymph, reaching in parallel its maximum levels in an hour. After that the drug concentration in the lymph persisted for 6 hours and markedly exceeded that in the blood. Adsorption of the lymph lowered the antibiotic concentration in it to 25 per cent indicating the necessity for increasing the drug therapeutic doses."}
{"id": "PMID:485114", "title": "[Gentamycin sulfate effectiveness and tolerance in patients with myelotoxic agranulocytosis].", "content": "Patients with myelotoxic agranulocytosis were treated with gentamicin administered intravenously as drop-wise infusions in a dose of 5 mg/kg body weight a day every 8 hours. The treatment course consisted of 7--24 days. No toxic reactions were observed. The therapeutic efficacy of gentamicin depended on the neutrophilic level and amounted to 50--60 per cent when the drug was used without identification of the causative agent. The prophylactic use of gentamicin decreased the infection incidence in patients with myelotoxic agranulocytosis, while the prophylactic efficacy of gentamicin was evident only when the number of the granulocytes was higher than 100/mm3 of the blood.", "contents": "[Gentamycin sulfate effectiveness and tolerance in patients with myelotoxic agranulocytosis]. Patients with myelotoxic agranulocytosis were treated with gentamicin administered intravenously as drop-wise infusions in a dose of 5 mg/kg body weight a day every 8 hours. The treatment course consisted of 7--24 days. No toxic reactions were observed. The therapeutic efficacy of gentamicin depended on the neutrophilic level and amounted to 50--60 per cent when the drug was used without identification of the causative agent. The prophylactic use of gentamicin decreased the infection incidence in patients with myelotoxic agranulocytosis, while the prophylactic efficacy of gentamicin was evident only when the number of the granulocytes was higher than 100/mm3 of the blood."}
{"id": "PMID:485115", "title": "[Prodigiozan aerosols in the treatment of nonspecific respiratory organ diseases in children].", "content": "High efficacy of prodigiozan, a bacterial polysaccharide used in the form of inhalation in combined therapy of 123 children at the age of 3 to 14 with nonspecific bronchopulmonary pathologic conditions was shown. The drug was administered by means of inhalators UI-I and PAI-I once every 4--5 days in the form of 0.02 per cent solution prepared in situ. The single and the course doses were 100--400 and 300--2000 micrograms respectively. The positive therapeutic effect was observed in 85.0 per cent of the patients: improvement of the patient state was registered 4--6 days earlier than that in the control group, the indices of the respiration mechanics and nonspecific immunity (titers of lysozyme and complement) also improved. The rate of viral respiratory diseases among the children treated with prodigiozan decreased 2.5 times. High efficacy and physiological character of the aerosol method for administration of prodigiozan used the the first time in pediatry provided its recommendation for wide use.", "contents": "[Prodigiozan aerosols in the treatment of nonspecific respiratory organ diseases in children]. High efficacy of prodigiozan, a bacterial polysaccharide used in the form of inhalation in combined therapy of 123 children at the age of 3 to 14 with nonspecific bronchopulmonary pathologic conditions was shown. The drug was administered by means of inhalators UI-I and PAI-I once every 4--5 days in the form of 0.02 per cent solution prepared in situ. The single and the course doses were 100--400 and 300--2000 micrograms respectively. The positive therapeutic effect was observed in 85.0 per cent of the patients: improvement of the patient state was registered 4--6 days earlier than that in the control group, the indices of the respiration mechanics and nonspecific immunity (titers of lysozyme and complement) also improved. The rate of viral respiratory diseases among the children treated with prodigiozan decreased 2.5 times. High efficacy and physiological character of the aerosol method for administration of prodigiozan used the the first time in pediatry provided its recommendation for wide use."}
{"id": "PMID:485116", "title": "[Formation of antibiotic 2562 by a culture of the actinomycete, Streptomyces griseovariabilis].", "content": "An actinomycetous culture 2562 inhibiting the growth of gramnegative bacteria was isolated from a soil sample. The culture was classified as Streptomyces griseovariabilis. It was found that culture 2562 produced an antibiotic belonging to the group of novobiocin. It consists of 2 components. One of them is identical to chlorobiocin and the other is a minor component of this group. Some parameters of the antibiotic complex production by strain 2562 under submerged conditions were studied. Nutrient media providing the predominant biosynthesis of the first (main) or the second component of the antibiotic were developed.", "contents": "[Formation of antibiotic 2562 by a culture of the actinomycete, Streptomyces griseovariabilis]. An actinomycetous culture 2562 inhibiting the growth of gramnegative bacteria was isolated from a soil sample. The culture was classified as Streptomyces griseovariabilis. It was found that culture 2562 produced an antibiotic belonging to the group of novobiocin. It consists of 2 components. One of them is identical to chlorobiocin and the other is a minor component of this group. Some parameters of the antibiotic complex production by strain 2562 under submerged conditions were studied. Nutrient media providing the predominant biosynthesis of the first (main) or the second component of the antibiotic were developed."}
{"id": "PMID:485117", "title": "[Effect of inorganic phosphate on levorin biosynthesis and on the mycelial makeup of Streptomyces levoris].", "content": "The effect of inorganic phosphate on biosynthesis of the polyenic antibiotic levorin by Streptomyces levoris and composition of the culture mycelium was studied. It was found that the synthetic medium with 0.4 mM of phosphate was optimal for growth of Str. levoris. When the concentration of phosphate was higher, the biomass increased, while the synthesis of levorin appeared to be inhibited and morphological changes in the culture were observed. Phosphate had a significant effect on the mycelium composition. When its concentration was increased 10 times as compared to the optimal one, the amounts of protein, RNA, total phosphorus and polyphosphates increased 1.3--1.4, 1.6--1.7, 2--3 and 10 times respectively, while the synthesis of levorin decreased 5 times. Changes in the lipid component of the mycelium were also observed. In the absence of inorganic phosphate in the medium the acetone precipitating fraction of the lipids contained 20--40 per cent of the phosphoruless compounds. During cultivation their portion increased up to 70--77 per cent. However, in the presence of its excess the polar lipids were represented only by phospholipids during the whole life cycle. The fatty acid spectrum of the lipids did not depend on the phosphate concentration and was represented mainly by saturated fatty acids with a branched chain of a series of iso- and anteiso-structures containing 14--18 carbon atoms.", "contents": "[Effect of inorganic phosphate on levorin biosynthesis and on the mycelial makeup of Streptomyces levoris]. The effect of inorganic phosphate on biosynthesis of the polyenic antibiotic levorin by Streptomyces levoris and composition of the culture mycelium was studied. It was found that the synthetic medium with 0.4 mM of phosphate was optimal for growth of Str. levoris. When the concentration of phosphate was higher, the biomass increased, while the synthesis of levorin appeared to be inhibited and morphological changes in the culture were observed. Phosphate had a significant effect on the mycelium composition. When its concentration was increased 10 times as compared to the optimal one, the amounts of protein, RNA, total phosphorus and polyphosphates increased 1.3--1.4, 1.6--1.7, 2--3 and 10 times respectively, while the synthesis of levorin decreased 5 times. Changes in the lipid component of the mycelium were also observed. In the absence of inorganic phosphate in the medium the acetone precipitating fraction of the lipids contained 20--40 per cent of the phosphoruless compounds. During cultivation their portion increased up to 70--77 per cent. However, in the presence of its excess the polar lipids were represented only by phospholipids during the whole life cycle. The fatty acid spectrum of the lipids did not depend on the phosphate concentration and was represented mainly by saturated fatty acids with a branched chain of a series of iso- and anteiso-structures containing 14--18 carbon atoms."}
{"id": "PMID:485118", "title": "[Mechanism of the myeloinhibitory effect of carminomycin].", "content": "The proliferative activity and level of aberrant mitoses in the cells of the bone marrow were studied experimentally on 223 noninbred mice treated with carminomycin administered intraperitoneally in single (LD50) and repeated doses. When the antibiotic was used in a single dose the values of the mitotic activity of the bone marrow elements did not correspond to the severity of depression and thir quantitative composition, which was explained by an impairement of the mitosis quality and possible interkinetic destruction of a significant part of both erythroid and immature myeloid cells capable of division at early stages after the exposure. At the same time the level of the bone marrow devastation under conditions of the treatment with repeated doses was mainly determined by inhibition of the erythronormoblast proliferative activity.", "contents": "[Mechanism of the myeloinhibitory effect of carminomycin]. The proliferative activity and level of aberrant mitoses in the cells of the bone marrow were studied experimentally on 223 noninbred mice treated with carminomycin administered intraperitoneally in single (LD50) and repeated doses. When the antibiotic was used in a single dose the values of the mitotic activity of the bone marrow elements did not correspond to the severity of depression and thir quantitative composition, which was explained by an impairement of the mitosis quality and possible interkinetic destruction of a significant part of both erythroid and immature myeloid cells capable of division at early stages after the exposure. At the same time the level of the bone marrow devastation under conditions of the treatment with repeated doses was mainly determined by inhibition of the erythronormoblast proliferative activity."}
{"id": "PMID:485119", "title": "[Effect of streptomycin sulfate on the process of higher nervous activity recovery in dogs in the postanesthesia period].", "content": "The effect of streptomycin on recovery of the higher nervous activity during the postanesthesia period was studied on 3 dogs with the method of the conditioned reflexes. It was found that streptomycin sulphate administered in a dose of 25 mg per 1 kg body weight induced no changes in the conditioned reflexes of the non-anesthetized animals. After ether anesthesia disorders in the conditioned and unconditioned reflexes, as well as differentiation was observed during the postanesthesia period. On an average they normalized in 7, 6 and 2 days respectively from the moment of anesthesia. When streptomycin sulphate was used during the post anesthesia period, both the strength and the character of the disorders in the conditioned reflexes changed. In this case recovery of the conditioned and unconditioned reflexes was registered in 2 days, while that of the differential inhibition was registered in 3--7 days from the moment of anesthesia.", "contents": "[Effect of streptomycin sulfate on the process of higher nervous activity recovery in dogs in the postanesthesia period]. The effect of streptomycin on recovery of the higher nervous activity during the postanesthesia period was studied on 3 dogs with the method of the conditioned reflexes. It was found that streptomycin sulphate administered in a dose of 25 mg per 1 kg body weight induced no changes in the conditioned reflexes of the non-anesthetized animals. After ether anesthesia disorders in the conditioned and unconditioned reflexes, as well as differentiation was observed during the postanesthesia period. On an average they normalized in 7, 6 and 2 days respectively from the moment of anesthesia. When streptomycin sulphate was used during the post anesthesia period, both the strength and the character of the disorders in the conditioned reflexes changed. In this case recovery of the conditioned and unconditioned reflexes was registered in 2 days, while that of the differential inhibition was registered in 3--7 days from the moment of anesthesia."}
{"id": "PMID:485120", "title": "[Pharmacokinetic study of rifampicin in the body of pregnant animals].", "content": "The study on distribution of 14C-rifampicin administered intramuscularly to pregnent animals showed that its concentrations in the blood, liver, kidneys, lungs and other organs did not practically change from those in nonpregnant animals. The concentration of 14C-rifampicin in the fetus organs was much lower than that in the organs of the adult animals. The liver and kidneys of the pregnant animals, as well as the fetus though to a less extent had a capacity for metabolism of 14C-rifampicin. The following products of biotransformation were detected: N-oxide of rifampicin, 25-deacetylrifampicin, 3-formylrifamycin SV and rifamycin SV.", "contents": "[Pharmacokinetic study of rifampicin in the body of pregnant animals]. The study on distribution of 14C-rifampicin administered intramuscularly to pregnent animals showed that its concentrations in the blood, liver, kidneys, lungs and other organs did not practically change from those in nonpregnant animals. The concentration of 14C-rifampicin in the fetus organs was much lower than that in the organs of the adult animals. The liver and kidneys of the pregnant animals, as well as the fetus though to a less extent had a capacity for metabolism of 14C-rifampicin. The following products of biotransformation were detected: N-oxide of rifampicin, 25-deacetylrifampicin, 3-formylrifamycin SV and rifamycin SV."}
{"id": "PMID:485121", "title": "[Dependence of the nephrotoxic effect of kanamycin on its concentration in the blood (an experimental study)].", "content": "The kinetics of changes in the urea nitrogen level of the serum was studied experimentally on narcotized cats with constant blood levels of kanamycin. A relationship between the intensity of the nephrotoxic effect of kanamycin and its blood level was found. On the basis of this relationship lower nephrotoxicity of kanamycin as compared to that of gentamicin and streptomycin under conditions of their constant blood levels was shown. However, the concentrations of gentamicin and kanamycin provided in the blood by their use in therapeutic doses differeing 3--4 times allow a conclusion that the nephrotoxic effect of kanamycin and gentamicin to be practically the same.", "contents": "[Dependence of the nephrotoxic effect of kanamycin on its concentration in the blood (an experimental study)]. The kinetics of changes in the urea nitrogen level of the serum was studied experimentally on narcotized cats with constant blood levels of kanamycin. A relationship between the intensity of the nephrotoxic effect of kanamycin and its blood level was found. On the basis of this relationship lower nephrotoxicity of kanamycin as compared to that of gentamicin and streptomycin under conditions of their constant blood levels was shown. However, the concentrations of gentamicin and kanamycin provided in the blood by their use in therapeutic doses differeing 3--4 times allow a conclusion that the nephrotoxic effect of kanamycin and gentamicin to be practically the same."}
{"id": "PMID:485123", "title": "Comparison of the activities of the new ureidopenicillins piperacillin, mezlocillin, azlocillin, and Bay k 4999 against gram-negative organisms.", "content": "The in vitro activities of the new ureidopenicillins piperacillin, mezlocillin, azlocillin, and Bay k 4999 were compared with those of ampicillin and ticarcillin against 336 Enterobacteriaceae, 109 nonfermenters, 55 Neisseria, and 28 Haemophilus influenzae isolates. Bay k 4999 displayed the largest spectrum of activity and had lower minimal inhibitory concentrations than any of the other penicillins against all of the species tested. Piperacillin showed the same spectrum but was slightly less active than Bay k 4999; it was slightly more effective than mezlocillin against Enterobacteriaceae and fully as active as azlocillin against Pseudomonas. All ureidopenicillins were substantially more active than ampicillin and ticarcillin. Isolates highly resistant to ampicillin or ticarcillin were also less susceptible to the ureidopenicillins.", "contents": "Comparison of the activities of the new ureidopenicillins piperacillin, mezlocillin, azlocillin, and Bay k 4999 against gram-negative organisms. The in vitro activities of the new ureidopenicillins piperacillin, mezlocillin, azlocillin, and Bay k 4999 were compared with those of ampicillin and ticarcillin against 336 Enterobacteriaceae, 109 nonfermenters, 55 Neisseria, and 28 Haemophilus influenzae isolates. Bay k 4999 displayed the largest spectrum of activity and had lower minimal inhibitory concentrations than any of the other penicillins against all of the species tested. Piperacillin showed the same spectrum but was slightly less active than Bay k 4999; it was slightly more effective than mezlocillin against Enterobacteriaceae and fully as active as azlocillin against Pseudomonas. All ureidopenicillins were substantially more active than ampicillin and ticarcillin. Isolates highly resistant to ampicillin or ticarcillin were also less susceptible to the ureidopenicillins."}
{"id": "PMID:485124", "title": "In vitro activity of sodium fusidate against anaerobic bacteria.", "content": "The microtiter broth dilution method was employed to determine the in vitro susceptibility of 525 recent clinical isolates of anaerobic bacteria to sodium fusidate. The minimal inhibitory concentrations of sodium fusidate ranged from </=0.06 to 1.0 mug/ml for 155 strains of anaerobic gram-positive rods and 130 strains of anaerobic gram-positive cocci. Minimal inhibitory concentrations ranging from </=0.06 to 32 mug/ml were observed for 240 strains of anaerobic gram-negative rods. Among the latter group a minimal inhibitory concentration of 16 mug/ml or greater was encountered with 16% of 45 Bacteroides fragilis strains, 19% of 32 Bacteroides thetaiotaomicron, and all 7 strains of Fusobacterium necrophorum. Minimal inhibitory concentrations for Veillonella parvula, the only gram-negative coccus tested, ranged from 0.5 to 8.0 mug/ml.", "contents": "In vitro activity of sodium fusidate against anaerobic bacteria. The microtiter broth dilution method was employed to determine the in vitro susceptibility of 525 recent clinical isolates of anaerobic bacteria to sodium fusidate. The minimal inhibitory concentrations of sodium fusidate ranged from </=0.06 to 1.0 mug/ml for 155 strains of anaerobic gram-positive rods and 130 strains of anaerobic gram-positive cocci. Minimal inhibitory concentrations ranging from </=0.06 to 32 mug/ml were observed for 240 strains of anaerobic gram-negative rods. Among the latter group a minimal inhibitory concentration of 16 mug/ml or greater was encountered with 16% of 45 Bacteroides fragilis strains, 19% of 32 Bacteroides thetaiotaomicron, and all 7 strains of Fusobacterium necrophorum. Minimal inhibitory concentrations for Veillonella parvula, the only gram-negative coccus tested, ranged from 0.5 to 8.0 mug/ml."}
{"id": "PMID:485125", "title": "Human pharmacology of cefotaxime (HR 756), a new cephalosporin.", "content": "Cefotaxime (HR 756) is a new semisynthetic parenteral cephalosporin with exceptional activity against gram-negative organisms and considerable stability against their beta-lactamases. To study its pharmacokinetic properties, 0.5-, 1-, and 2-g doses were administered to each of six volunteers intravenously over 15 min, followed by a sustaining infusions of 0.5, 1, and 2 g/h, respectively, for 3 consecutive hours. The loading doses produced mean peak levels of 41, 93, and 160 mug/ml, and mean steady-state serum concentrations were 27, 64, and 138 mug/ml, respectively. The mean terminal half-life was 75 +/- 7 min. The total volume of distribution averaged 0.22 +/- 0.03 liters/kg of body weight. Total body and renal clearances were 232 +/- 30 and 145 +/- 24 ml/min per 1.73 m(2), respectively; 63 +/- 9% of the administered dose was excreted through the kidneys in 24 h. To determine the effect of cefotaxime on the renal tubules, urinary alanine aminopeptidase excretion was measured before, during, and after the infusions. It remained within the normal range in all instances; however, 48 +/- 14% of the total daily alanine aminopeptidase output was recovered during the infusion period. Side effects were dose related and included fatigue, loose stools, and night sweats. No significant changes in hematology, serum chemistry, or urinalysis were recorded.", "contents": "Human pharmacology of cefotaxime (HR 756), a new cephalosporin. Cefotaxime (HR 756) is a new semisynthetic parenteral cephalosporin with exceptional activity against gram-negative organisms and considerable stability against their beta-lactamases. To study its pharmacokinetic properties, 0.5-, 1-, and 2-g doses were administered to each of six volunteers intravenously over 15 min, followed by a sustaining infusions of 0.5, 1, and 2 g/h, respectively, for 3 consecutive hours. The loading doses produced mean peak levels of 41, 93, and 160 mug/ml, and mean steady-state serum concentrations were 27, 64, and 138 mug/ml, respectively. The mean terminal half-life was 75 +/- 7 min. The total volume of distribution averaged 0.22 +/- 0.03 liters/kg of body weight. Total body and renal clearances were 232 +/- 30 and 145 +/- 24 ml/min per 1.73 m(2), respectively; 63 +/- 9% of the administered dose was excreted through the kidneys in 24 h. To determine the effect of cefotaxime on the renal tubules, urinary alanine aminopeptidase excretion was measured before, during, and after the infusions. It remained within the normal range in all instances; however, 48 +/- 14% of the total daily alanine aminopeptidase output was recovered during the infusion period. Side effects were dose related and included fatigue, loose stools, and night sweats. No significant changes in hematology, serum chemistry, or urinalysis were recorded."}
{"id": "PMID:485126", "title": "In vitro antiherpesviral activity of 5-alkyl derivatives of 1-beta-D-arabinofuranosyluracil.", "content": "Several 5-alkyl derivatives of 1-beta-d-arabinofuranosyluracil (araU) were tested for antiherpesviral activity and inhibitory action on cell growth in human embryonic lung fibroblasts. 1-beta-d-Arabinofuranosylcytosine, 9-beta-d-arabinofuranosyladenine, and 5-iododeoxyuridine (IUdR) were included as reference materials. Among the 5-alkyl derivatives of araU, arabinosylthymine was the most active, followed by 5-ethyl- and 5-propyl-araU. 5-Ethyl-araU was as active as IUdR and more active than 9-beta-d-arabinofuranosyladenine against herpes simplex virus (HSV) type 1 and did not inhibit cell growth at a concentration as high as 1,000 mug/ml. 5-Butyl- and 5-methoxymethyl-araU, as well as araU, exhibited relatively low activity. The araU derivatives tested were as active against HSV WT-34, an isolate from a patient with keratitis, as against HSV type 1. Against an IUdR-resistant isolate, HSV WT-20, arabinosylthymine was less inhibitory than IUdR. Deoxyribonucleic acid synthesis in HSV type 1-infected cells was markedly inhibited by arabinosylthymine, IUdR, and 5-ethyl-araU, whereas cellular deoxyribonucleic acid synthesis in uninfected cells was significantly inhibited by IUdR but not by arabinosylthymine or 5-ethyl-araU.", "contents": "In vitro antiherpesviral activity of 5-alkyl derivatives of 1-beta-D-arabinofuranosyluracil. Several 5-alkyl derivatives of 1-beta-d-arabinofuranosyluracil (araU) were tested for antiherpesviral activity and inhibitory action on cell growth in human embryonic lung fibroblasts. 1-beta-d-Arabinofuranosylcytosine, 9-beta-d-arabinofuranosyladenine, and 5-iododeoxyuridine (IUdR) were included as reference materials. Among the 5-alkyl derivatives of araU, arabinosylthymine was the most active, followed by 5-ethyl- and 5-propyl-araU. 5-Ethyl-araU was as active as IUdR and more active than 9-beta-d-arabinofuranosyladenine against herpes simplex virus (HSV) type 1 and did not inhibit cell growth at a concentration as high as 1,000 mug/ml. 5-Butyl- and 5-methoxymethyl-araU, as well as araU, exhibited relatively low activity. The araU derivatives tested were as active against HSV WT-34, an isolate from a patient with keratitis, as against HSV type 1. Against an IUdR-resistant isolate, HSV WT-20, arabinosylthymine was less inhibitory than IUdR. Deoxyribonucleic acid synthesis in HSV type 1-infected cells was markedly inhibited by arabinosylthymine, IUdR, and 5-ethyl-araU, whereas cellular deoxyribonucleic acid synthesis in uninfected cells was significantly inhibited by IUdR but not by arabinosylthymine or 5-ethyl-araU."}
{"id": "PMID:485127", "title": "Etiology of tetracycline-associated pseudomembranous colitis in hamsters.", "content": "Tetracyclines were implicated in the 1950s in induction of protracted diarrhea and pseudomembranous colitis. Because the pathogenetic mechanism of these illnesses has been questioned recently, we studied tetracycline in hamster models of antibiotic-associated colitis. Orogastric administration of tetracycline caused diarrhea and death, with evidence of hemorrhagic typhlitis. Filtrates of cecal contents were toxic when inoculated into normal hamsters and cell culture monolayers, and toxicity was neutralized with Clostridium sordellii antitoxin. Tetracycline-resistant C. difficile was cultured from stools of these hamsters, but Staphylococcus aureus was not isolated. The value of tetracycline for treatment or prevention of clindamycin-induced colitis in hamsters was also studied, and it was found that daily orogastric administration of tetracycline was poorly protective against clindamycin-induced colitis.", "contents": "Etiology of tetracycline-associated pseudomembranous colitis in hamsters. Tetracyclines were implicated in the 1950s in induction of protracted diarrhea and pseudomembranous colitis. Because the pathogenetic mechanism of these illnesses has been questioned recently, we studied tetracycline in hamster models of antibiotic-associated colitis. Orogastric administration of tetracycline caused diarrhea and death, with evidence of hemorrhagic typhlitis. Filtrates of cecal contents were toxic when inoculated into normal hamsters and cell culture monolayers, and toxicity was neutralized with Clostridium sordellii antitoxin. Tetracycline-resistant C. difficile was cultured from stools of these hamsters, but Staphylococcus aureus was not isolated. The value of tetracycline for treatment or prevention of clindamycin-induced colitis in hamsters was also studied, and it was found that daily orogastric administration of tetracycline was poorly protective against clindamycin-induced colitis."}
{"id": "PMID:485128", "title": "Treatment of bacterial meningitis with intravenous amoxicillin.", "content": "Eleven children with bacterial meningitis were treated intravenously with amoxicillin sodium to evaluate the efficacy of the parenteral form of amoxicillin for this serious infection and to measure the penetration of the drug into cerebrospinal fluid (CSF). The infecting organisms were Haemophilus influenzae in nine cases and Streptococcus pneumoniae in two. Nine patients had optimal responses to amoxicillin sodium, 200 mg/kg per day for 14 days. Bacteria were also eradicated from CSF of the other two, but one experienced fever and culture-negative CSF pleocytosis after cessation of amoxicillin, and the other developed H. influenzae empyema 2 weeks after termination of therapy. By comparison, 7 of 10 children with meningitis responded optimally to ampicillin (nonrandomized design) during the period of study. The mean peak CSF concentration of amoxicillin was 3.14 mug/ml (ca. 7% of the concomitant mean peak serum level) early during therapy. However, meningeal penetration of the drug declined to a mean peak of 0.63 mug/ml on the final day of therapy. Mild transient neutropenia, noted in five patients, was the most common side effect of amoxicillin sodium therapy; five patients treated with ampicillin also experienced reversible neutropenia. Thus, intravenous amoxicillin sodium provided therapy for bacterial meningitis comparable to that of ampicillin in this limited case-control study.", "contents": "Treatment of bacterial meningitis with intravenous amoxicillin. Eleven children with bacterial meningitis were treated intravenously with amoxicillin sodium to evaluate the efficacy of the parenteral form of amoxicillin for this serious infection and to measure the penetration of the drug into cerebrospinal fluid (CSF). The infecting organisms were Haemophilus influenzae in nine cases and Streptococcus pneumoniae in two. Nine patients had optimal responses to amoxicillin sodium, 200 mg/kg per day for 14 days. Bacteria were also eradicated from CSF of the other two, but one experienced fever and culture-negative CSF pleocytosis after cessation of amoxicillin, and the other developed H. influenzae empyema 2 weeks after termination of therapy. By comparison, 7 of 10 children with meningitis responded optimally to ampicillin (nonrandomized design) during the period of study. The mean peak CSF concentration of amoxicillin was 3.14 mug/ml (ca. 7% of the concomitant mean peak serum level) early during therapy. However, meningeal penetration of the drug declined to a mean peak of 0.63 mug/ml on the final day of therapy. Mild transient neutropenia, noted in five patients, was the most common side effect of amoxicillin sodium therapy; five patients treated with ampicillin also experienced reversible neutropenia. Thus, intravenous amoxicillin sodium provided therapy for bacterial meningitis comparable to that of ampicillin in this limited case-control study."}
{"id": "PMID:485129", "title": "Clinical and bacteriological evaluation of cefoxitin therapy in children.", "content": "Cefoxitin, a parenteral cephamycin beta-lactam antibiotic, was evaluated for safety and efficacy in children with bacterial infections other than meningitis. Twentysix patients between 3 months and 7 years of age were treated with 80 to 160 mg/kg per day. The most common diagnoses were cellulitis (13 patients), pneumonia (5 patients), and bone and joint infection (4 patients). Nine patients were bacteremic. The most frequently recovered pathogens were Staphylococcus aureus (six patients), Haemophilus influenzae (four patients), and Streptococcus pneumoniae (three patients). All organisms were susceptible to cefoxitin. All 26 children were considered improved or cured. No severe adverse reactions were encountered. Phlebitis (4%), eosinophilia (12%), and elevated liver function tests (4%) were associated with therapy. Cefoxitin appears to be a safe, effective, and well-tolerated antibiotic when used in children with susceptible bacterial infections other than meningitis.", "contents": "Clinical and bacteriological evaluation of cefoxitin therapy in children. Cefoxitin, a parenteral cephamycin beta-lactam antibiotic, was evaluated for safety and efficacy in children with bacterial infections other than meningitis. Twentysix patients between 3 months and 7 years of age were treated with 80 to 160 mg/kg per day. The most common diagnoses were cellulitis (13 patients), pneumonia (5 patients), and bone and joint infection (4 patients). Nine patients were bacteremic. The most frequently recovered pathogens were Staphylococcus aureus (six patients), Haemophilus influenzae (four patients), and Streptococcus pneumoniae (three patients). All organisms were susceptible to cefoxitin. All 26 children were considered improved or cured. No severe adverse reactions were encountered. Phlebitis (4%), eosinophilia (12%), and elevated liver function tests (4%) were associated with therapy. Cefoxitin appears to be a safe, effective, and well-tolerated antibiotic when used in children with susceptible bacterial infections other than meningitis."}
{"id": "PMID:485130", "title": "New rapid assay for nafcillin in serum by spectrofluorometry.", "content": "A new, rapid method for measuring serum levels of nafcillin by spectrofluorometry is described. The method involves extraction of 2 ml of acidified serum with n-butyl chloride, subjecting the organic solvent layer to excitation at 340 nm, and measuring the relative intensity of emission fluorescence at 380 nm. An excellent linear correlation exists between serum levels of nafcillin and the relative intensity in a drug concentration range of 0.25 to 150 mug/ml. The results obtained by this spectrofluorometric technique are in complete accord with those obtained by the conventional microbiological assay using Staphylococcus aureus ATCC 6538P. The method is not interfered with by elevated levels of endogenous metabolic products or the presence of other drugs, including a number of antimicrobial agents. The assay is interfered with, however, by the presence of salicylates, for which appropriate correction can easily be made. A salicylate assay employing a spectrofluorometric technique is also described.", "contents": "New rapid assay for nafcillin in serum by spectrofluorometry. A new, rapid method for measuring serum levels of nafcillin by spectrofluorometry is described. The method involves extraction of 2 ml of acidified serum with n-butyl chloride, subjecting the organic solvent layer to excitation at 340 nm, and measuring the relative intensity of emission fluorescence at 380 nm. An excellent linear correlation exists between serum levels of nafcillin and the relative intensity in a drug concentration range of 0.25 to 150 mug/ml. The results obtained by this spectrofluorometric technique are in complete accord with those obtained by the conventional microbiological assay using Staphylococcus aureus ATCC 6538P. The method is not interfered with by elevated levels of endogenous metabolic products or the presence of other drugs, including a number of antimicrobial agents. The assay is interfered with, however, by the presence of salicylates, for which appropriate correction can easily be made. A salicylate assay employing a spectrofluorometric technique is also described."}
{"id": "PMID:485131", "title": "In vitro susceptibilities of Naegleria fowleri strain HB-1 to selected antimicrobial agents, singly and in combination.", "content": "The overall prognosis of primary amoebic meningoencephalitis remains poor. The results of this study support previous finding that amphotericin B is the most efficacious drug against the Naegleria species in in vitro testing. In addition, the methyl ester of amphotericin B, a new derivative, also appears to be an effective agent. Of the drug combinations studied, amphotericin B plus minocycline and amphotericin B plus tetracycline showed synergy. The clinical significance of these findings remains to be determined.", "contents": "In vitro susceptibilities of Naegleria fowleri strain HB-1 to selected antimicrobial agents, singly and in combination. The overall prognosis of primary amoebic meningoencephalitis remains poor. The results of this study support previous finding that amphotericin B is the most efficacious drug against the Naegleria species in in vitro testing. In addition, the methyl ester of amphotericin B, a new derivative, also appears to be an effective agent. Of the drug combinations studied, amphotericin B plus minocycline and amphotericin B plus tetracycline showed synergy. The clinical significance of these findings remains to be determined."}
{"id": "PMID:485132", "title": "Spectrophotometric assay for amikacin using purified kanamycin acetyltransferase.", "content": "A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme from R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, that is measured in the visible spectrum. The system complements an earlier assay for gentamicin-related antibiotics thereby facilitating the rapid measurement of the concentrations of all clinically important aminoglycosides in serum.", "contents": "Spectrophotometric assay for amikacin using purified kanamycin acetyltransferase. A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme from R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, that is measured in the visible spectrum. The system complements an earlier assay for gentamicin-related antibiotics thereby facilitating the rapid measurement of the concentrations of all clinically important aminoglycosides in serum."}
{"id": "PMID:485133", "title": "Inhibition by ethambutol of mycolic acid transfer into the cell wall of Mycobacterium smegmatis.", "content": "Ethambutol simultaneously inhibited the transfer (presumably via mycolyl acetyl trehalose) of mycolic acids into the cell wall and stimulated the synthesis of trehalose dimycolates of Mycobacterium smegmatis. Structural similarities of the drug and mycolyl acetyl trehalose suggested that competitive inhibition was involved.", "contents": "Inhibition by ethambutol of mycolic acid transfer into the cell wall of Mycobacterium smegmatis. Ethambutol simultaneously inhibited the transfer (presumably via mycolyl acetyl trehalose) of mycolic acids into the cell wall and stimulated the synthesis of trehalose dimycolates of Mycobacterium smegmatis. Structural similarities of the drug and mycolyl acetyl trehalose suggested that competitive inhibition was involved."}
{"id": "PMID:485134", "title": "Toxicity of parasporal crystals of Bacillus thuringiensis to the Indian meal moth, Plodia interpunctella.", "content": "Toxicity of Bacillus thuringiensis parasporal crystals to the Indian meal moth, Plodia interpunctella, is described. The numbers of insects killed were in relation to crystal dry weight. Mortality was determined by comparing adult emergence in diets treated with crystals to emergence in untreated diets. There was only a 30% survival at an application of 0.414 microgram/cm2, and the mean 50% lethal concentration value was found to be 0.299 microgram/cm2. The use of emergence data has provided a reliable and reproducible bioassay for comparing relative toxicities of crystals, spores, and other cellular components to this economically important insect.", "contents": "Toxicity of parasporal crystals of Bacillus thuringiensis to the Indian meal moth, Plodia interpunctella. Toxicity of Bacillus thuringiensis parasporal crystals to the Indian meal moth, Plodia interpunctella, is described. The numbers of insects killed were in relation to crystal dry weight. Mortality was determined by comparing adult emergence in diets treated with crystals to emergence in untreated diets. There was only a 30% survival at an application of 0.414 microgram/cm2, and the mean 50% lethal concentration value was found to be 0.299 microgram/cm2. The use of emergence data has provided a reliable and reproducible bioassay for comparing relative toxicities of crystals, spores, and other cellular components to this economically important insect."}
{"id": "PMID:485135", "title": "Isolation and characterization of a bacteriophage specific for Sphaerotilus natans which contain an unusual base in its deoxyribonucleic acid.", "content": "A bacteriophage was isolated specific for Sphaerotilus natans, an organism for which bacteriophages have not been previously described. This phage (designated SN1) was found to infect both the single-cell (S-type) and filamentous (R-type) forms of the host, although the sheath appeared to provide R-type cells with a degree of physical protection from infection. SN1 had a hexagonal head and a long flexible tail resembling most closely the phages in Bradley's group B. The nucleic acid was found to be deoxyribonucleic acid and contained an unusual base which substituted for 35% of the guanine. Deoxyribonucleic acid base composition was 56% cytosine plus guanine.", "contents": "Isolation and characterization of a bacteriophage specific for Sphaerotilus natans which contain an unusual base in its deoxyribonucleic acid. A bacteriophage was isolated specific for Sphaerotilus natans, an organism for which bacteriophages have not been previously described. This phage (designated SN1) was found to infect both the single-cell (S-type) and filamentous (R-type) forms of the host, although the sheath appeared to provide R-type cells with a degree of physical protection from infection. SN1 had a hexagonal head and a long flexible tail resembling most closely the phages in Bradley's group B. The nucleic acid was found to be deoxyribonucleic acid and contained an unusual base which substituted for 35% of the guanine. Deoxyribonucleic acid base composition was 56% cytosine plus guanine."}
{"id": "PMID:485136", "title": "Identification of the naturally occurring isomer of zearalenol produced by Fusarium roseum 'Gibbosum' in rice culture.", "content": "One diastereomer of trans-zearalenol [2,4-dihydroxy-6-(6,10-dihydroxy-trans-1-undecenyl)-benzoic acid-mu-lactone] was isolated from cultures of Fusarium roseum 'Gibbosum.' This strongly estrogenic metabolite was identified by analysis of its mass spectrum and its behavior in thin-layer, high-pressure liquid and gas-liquid chromatographic systems. The concentration of zearalenol in cultures was 563 mu g/g, or 7% of the 8,000-mu g/g zearalenone content, while the two diastereomers of 8'-hydroxyzearalenone each occurred at 3% of the zearalenone level. Of the two possible diastereomers of zearalenol, the one occurring in cultures was identical to the low-melting-point (171 degrees C) isomer (alpha) obtained by synthesis. In the rat uterus bioassay, the alpha zearalenol isomer was three times more estrogenic than zearalenone while the beta isomer was equal in activity in zearalenone. The two diastereomers of zearalenol can be distinguished from each other by the intensity of the m/e+ 302 fragment of the mass spectrum of the pure underivatized compound.", "contents": "Identification of the naturally occurring isomer of zearalenol produced by Fusarium roseum 'Gibbosum' in rice culture. One diastereomer of trans-zearalenol [2,4-dihydroxy-6-(6,10-dihydroxy-trans-1-undecenyl)-benzoic acid-mu-lactone] was isolated from cultures of Fusarium roseum 'Gibbosum.' This strongly estrogenic metabolite was identified by analysis of its mass spectrum and its behavior in thin-layer, high-pressure liquid and gas-liquid chromatographic systems. The concentration of zearalenol in cultures was 563 mu g/g, or 7% of the 8,000-mu g/g zearalenone content, while the two diastereomers of 8'-hydroxyzearalenone each occurred at 3% of the zearalenone level. Of the two possible diastereomers of zearalenol, the one occurring in cultures was identical to the low-melting-point (171 degrees C) isomer (alpha) obtained by synthesis. In the rat uterus bioassay, the alpha zearalenol isomer was three times more estrogenic than zearalenone while the beta isomer was equal in activity in zearalenone. The two diastereomers of zearalenol can be distinguished from each other by the intensity of the m/e+ 302 fragment of the mass spectrum of the pure underivatized compound."}
{"id": "PMID:485137", "title": "Multiple antibiotic resistance in Rhizobium japonicum.", "content": "A total of 48 strains of the soil bacterium Rhizobium japonicum were screened for their response to several widely used antibiotics. Over 60% of the strains were resistant to chloramphenicol, polymyxin B, and erythromycin, and 47% or more of the strains were resistant to neomycin and penicillin G, when tested by disk assay procedures. The most common grouping of resistances in strains was simultaneous resistance to tetracycline, penicillin G, neomycin, chloramphenicol, and streptomycin (25% of all strains tested). The occurrence of multiple drug resistance in a soil bacterium that is not a vertebrate pathogen suggests that chemotherapeutic use of antibiotics is not required for the development of multiple drug resistance.", "contents": "Multiple antibiotic resistance in Rhizobium japonicum. A total of 48 strains of the soil bacterium Rhizobium japonicum were screened for their response to several widely used antibiotics. Over 60% of the strains were resistant to chloramphenicol, polymyxin B, and erythromycin, and 47% or more of the strains were resistant to neomycin and penicillin G, when tested by disk assay procedures. The most common grouping of resistances in strains was simultaneous resistance to tetracycline, penicillin G, neomycin, chloramphenicol, and streptomycin (25% of all strains tested). The occurrence of multiple drug resistance in a soil bacterium that is not a vertebrate pathogen suggests that chemotherapeutic use of antibiotics is not required for the development of multiple drug resistance."}
{"id": "PMID:485138", "title": "Cyclitol utilization associated with the presence of Klebsielleae in botanical environments.", "content": "Bacteria of the tribe Klebsielleae are capable of metabolizing the cyclitols myoinositol, sequoyitol, and pinitol, which are present in aqueous extracts of redwood. Of the combined Klebsiella isolates from clinical and environmental origins, 100% (138/138), 97% (34/35), and 86% (119/138) fermented inositol, sequoyitol, and pinitol, respectively. These compounds were also used as a sole source of carbon and energy by Klebsiella. Similar results were obtained with Enterobacter isolates, but most other enteric bacteria tested could not metabolize cyclitols. Strains of Klebsiella multiplied to levels exceeding 10(5)/ml in aqueous extracts of nonsterile redwood within 6 days. Most other enteric bacteria did not grow in these extracts. Cyclitol metabolism was found to correlate well with the ability to multiply in redwood extract in the presence of cyclitol-negative indigenous bacteria. The capacity to use cyclitols, which are present in a variety of plant material, might afford Klebsielleae of both environmental and clinical origins an advantage in competing for nutrients and colonizing botanical environments.", "contents": "Cyclitol utilization associated with the presence of Klebsielleae in botanical environments. Bacteria of the tribe Klebsielleae are capable of metabolizing the cyclitols myoinositol, sequoyitol, and pinitol, which are present in aqueous extracts of redwood. Of the combined Klebsiella isolates from clinical and environmental origins, 100% (138/138), 97% (34/35), and 86% (119/138) fermented inositol, sequoyitol, and pinitol, respectively. These compounds were also used as a sole source of carbon and energy by Klebsiella. Similar results were obtained with Enterobacter isolates, but most other enteric bacteria tested could not metabolize cyclitols. Strains of Klebsiella multiplied to levels exceeding 10(5)/ml in aqueous extracts of nonsterile redwood within 6 days. Most other enteric bacteria did not grow in these extracts. Cyclitol metabolism was found to correlate well with the ability to multiply in redwood extract in the presence of cyclitol-negative indigenous bacteria. The capacity to use cyclitols, which are present in a variety of plant material, might afford Klebsielleae of both environmental and clinical origins an advantage in competing for nutrients and colonizing botanical environments."}
{"id": "PMID:485139", "title": "Microbial metabolism of carbon monoxide in culture and in soil.", "content": "Nocardia salmonicolor readily oxidized CO to CO2. Slight activity was found among species of Actinoplanes, Agromyces, Microbispora, Mycobacterium, and other nocardias, and no oxidation was detected in the algae, fungi, and other bacteria tested. Carbon monoxide was oxidized rapidly to CO2 in the dark in two soils incubated in air or under flooded conditions, but little of the 14C from 14CO was incorporated into the organic fraction of these soils. The reaction was microbial because appreciable CO was not converted to CO2 in autoclaved or gamma-irradiated soil. Heating the soil for 25 min at 70 degrees C destroyed its CO-oxidizing activity. The incorporation of 14CO2 into the cells of microorganisms in soil and soil suspension was not enhanced by incubating the samples in the presence of CO, suggesting that CO oxidation was not the result of autotrophic metabolism. The oxidation of 17 mu 1 of CO per liter in the head space was nearly complete in 6 h in soil incubated in air or anaerobically.", "contents": "Microbial metabolism of carbon monoxide in culture and in soil. Nocardia salmonicolor readily oxidized CO to CO2. Slight activity was found among species of Actinoplanes, Agromyces, Microbispora, Mycobacterium, and other nocardias, and no oxidation was detected in the algae, fungi, and other bacteria tested. Carbon monoxide was oxidized rapidly to CO2 in the dark in two soils incubated in air or under flooded conditions, but little of the 14C from 14CO was incorporated into the organic fraction of these soils. The reaction was microbial because appreciable CO was not converted to CO2 in autoclaved or gamma-irradiated soil. Heating the soil for 25 min at 70 degrees C destroyed its CO-oxidizing activity. The incorporation of 14CO2 into the cells of microorganisms in soil and soil suspension was not enhanced by incubating the samples in the presence of CO, suggesting that CO oxidation was not the result of autotrophic metabolism. The oxidation of 17 mu 1 of CO per liter in the head space was nearly complete in 6 h in soil incubated in air or anaerobically."}
{"id": "PMID:485140", "title": "Dichloran-rose bengal medium for enumeration and isolation of molds from foods.", "content": "Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. Dichloran (2,6 dichloro-4-nitroaniline) restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds. Three Rhizopus and Mucor species were encountered that were not inhibited by dichloran; these were controlled by the addition of rose bengal. The optimal medium, designated DRBC, contained 2 micrograms of dichloran and 25 micrograms of rose bengal per ml. DRBC, in pure culture tests and with food samples, restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media.", "contents": "Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. Dichloran (2,6 dichloro-4-nitroaniline) restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds. Three Rhizopus and Mucor species were encountered that were not inhibited by dichloran; these were controlled by the addition of rose bengal. The optimal medium, designated DRBC, contained 2 micrograms of dichloran and 25 micrograms of rose bengal per ml. DRBC, in pure culture tests and with food samples, restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media."}
{"id": "PMID:485141", "title": "Resolution of Penicillium roqueforti toxin and eremofortins A, B, and C by high-performance liquid chromatography.", "content": "A method for the quantitative analysis of Penicillium roqueforti toxin using a high-performance liquid chromatography system is proposed.", "contents": "Resolution of Penicillium roqueforti toxin and eremofortins A, B, and C by high-performance liquid chromatography. A method for the quantitative analysis of Penicillium roqueforti toxin using a high-performance liquid chromatography system is proposed."}
{"id": "PMID:485142", "title": "L-Glutamine formation by Flavobacterium rigense.", "content": "A penicillin-resistant mutant of Flavobacterium rigense designated as strain 703, FERM-P no. 3628, was obtained after ultraviolet treatment of F. rigense FERM-P no. 3556. The parent strain produces 0-2-hydroxypropylhomoserine from 1,2-propanediol. The mutant was found to be a good producer of L-glutamine. The physiological characteristics of strain 703 were different from the general group of L-glutamic acid-producing bacteria. Strain 703 required L-tryptophan and thiamine but not biotin for its growth. L-Glutamine formation on a specific basis, however, was independent of L-tryptophan and thiamine. Biotin and penicillin were also not effective. Only ammonium fumarate acted as an effective factor on L-glutamine formation. Accumulation of L-glutamine by strain 703 was 10 mg/ml at 30 degrees C for 48 h in a chemically defined medium containing 3% diammonium fumarate.", "contents": "L-Glutamine formation by Flavobacterium rigense. A penicillin-resistant mutant of Flavobacterium rigense designated as strain 703, FERM-P no. 3628, was obtained after ultraviolet treatment of F. rigense FERM-P no. 3556. The parent strain produces 0-2-hydroxypropylhomoserine from 1,2-propanediol. The mutant was found to be a good producer of L-glutamine. The physiological characteristics of strain 703 were different from the general group of L-glutamic acid-producing bacteria. Strain 703 required L-tryptophan and thiamine but not biotin for its growth. L-Glutamine formation on a specific basis, however, was independent of L-tryptophan and thiamine. Biotin and penicillin were also not effective. Only ammonium fumarate acted as an effective factor on L-glutamine formation. Accumulation of L-glutamine by strain 703 was 10 mg/ml at 30 degrees C for 48 h in a chemically defined medium containing 3% diammonium fumarate."}
{"id": "PMID:485143", "title": "Bacterial formation of omega-muricholic acid in rats.", "content": "In the feces of conventional rats, the amount of omega-muricholic and hyodeoxycholic acids vary according to the diet. To understand this phenomenon, we investigated the bacterial formation of these bile acids. The present paper reports the first isolation, from conventional rat feces, of a strain of Clostridium group III which transforms beta-muricholic acid, the main bile acid in germfree rats, into omega-muricholic acid.", "contents": "Bacterial formation of omega-muricholic acid in rats. In the feces of conventional rats, the amount of omega-muricholic and hyodeoxycholic acids vary according to the diet. To understand this phenomenon, we investigated the bacterial formation of these bile acids. The present paper reports the first isolation, from conventional rat feces, of a strain of Clostridium group III which transforms beta-muricholic acid, the main bile acid in germfree rats, into omega-muricholic acid."}
{"id": "PMID:485144", "title": "Comparison of the efficacy of steam sterilization indicators.", "content": "Twenty-one commercially available chemical steam sterilization indicators were processed in an empty autoclave for various times at temperatures between 240 and 270 degrees F (ca. 116 and 132 degrees C). The time required to reach a sterilized reading at each temperature was plotted on a semilogarithmic time-temperature plot and compared with the time-temperature sterilization curve for Bacillus stearothermophilus. Five of the indicators had time-temperature kinetics similar to those of B. stearothermophilus, but three of these overestimated the effect of processing. Two of the indicators overestimated the effect of processing and were less sensitive to temperature changes when was B. stearothermophilus. Thirteen of the indicators had time-temperature curves that crossed the B. stearothermophilus plot. One indicator produced such ambiguous results that no determinations could be made with it. Out of 21 indicators tested, only 2 appear to be capable of accurately integrating the time-temperature effect at temperatures between 240 and 270 degrees F. The other indicators should be used only after careful analysis of their suitability for use at a given temperature.", "contents": "Comparison of the efficacy of steam sterilization indicators. Twenty-one commercially available chemical steam sterilization indicators were processed in an empty autoclave for various times at temperatures between 240 and 270 degrees F (ca. 116 and 132 degrees C). The time required to reach a sterilized reading at each temperature was plotted on a semilogarithmic time-temperature plot and compared with the time-temperature sterilization curve for Bacillus stearothermophilus. Five of the indicators had time-temperature kinetics similar to those of B. stearothermophilus, but three of these overestimated the effect of processing. Two of the indicators overestimated the effect of processing and were less sensitive to temperature changes when was B. stearothermophilus. Thirteen of the indicators had time-temperature curves that crossed the B. stearothermophilus plot. One indicator produced such ambiguous results that no determinations could be made with it. Out of 21 indicators tested, only 2 appear to be capable of accurately integrating the time-temperature effect at temperatures between 240 and 270 degrees F. The other indicators should be used only after careful analysis of their suitability for use at a given temperature."}
{"id": "PMID:485145", "title": "Heat inactivation of catalase from Staphylococcus aureus MF-31.", "content": "The effects of heat on catalase from Staphylococcus aureus lysates were examined. Catalase activity increased with increasing concentrations of potassium phosphate buffer, when heated at temperatures between 50 and 65 degrees C for 10 min. Inactivation of catalase by NaCl during heating was demonstrated. Extended heating of S. aureus cells at 52 degrees C resulted in a slight decrease in catalase activity of the resultant lysates. This decrease was more pronounced in the presence of salt. Heating at 62 degrees C caused a decrease in catalase activity, but not complete inactivation. These results implicate the combined effects of heat, and NaCl in the inactivation of catalase from S. aureus. The findings are consistent with the hypothesis that H2O2 may accumulate as a result of decreased catalase activity and be responsible for the decreased colony-forming ability of stressed S. aureus.", "contents": "Heat inactivation of catalase from Staphylococcus aureus MF-31. The effects of heat on catalase from Staphylococcus aureus lysates were examined. Catalase activity increased with increasing concentrations of potassium phosphate buffer, when heated at temperatures between 50 and 65 degrees C for 10 min. Inactivation of catalase by NaCl during heating was demonstrated. Extended heating of S. aureus cells at 52 degrees C resulted in a slight decrease in catalase activity of the resultant lysates. This decrease was more pronounced in the presence of salt. Heating at 62 degrees C caused a decrease in catalase activity, but not complete inactivation. These results implicate the combined effects of heat, and NaCl in the inactivation of catalase from S. aureus. The findings are consistent with the hypothesis that H2O2 may accumulate as a result of decreased catalase activity and be responsible for the decreased colony-forming ability of stressed S. aureus."}
{"id": "PMID:485146", "title": "Colonization of a portion of the bovine tongue by unusual filamentous bacteria.", "content": "Tongue samples from cattle on varied diets and ranging in age from 2 months to adult were studied by transmission and scanning electron microscopy to observe the in situ distribution and adhesion patterns of two readily identifiable genera of filamentous bacterial. The two, both members of the Simonsiellaceae, adhere to the epithelium by means of fibers which are produced on one side of the bacterial filaments and subsequently display a sidedness in their manner of adhesion to epithelial surfaces. Other bacterial populations found on the tongue were normally members of chains and seldom present as single cells. This suggests that filamentous or chain-forming bacteria may have a selective advantage over single bacteria in their ability to colonize and remain attached to the epithelium of the tongue.", "contents": "Colonization of a portion of the bovine tongue by unusual filamentous bacteria. Tongue samples from cattle on varied diets and ranging in age from 2 months to adult were studied by transmission and scanning electron microscopy to observe the in situ distribution and adhesion patterns of two readily identifiable genera of filamentous bacterial. The two, both members of the Simonsiellaceae, adhere to the epithelium by means of fibers which are produced on one side of the bacterial filaments and subsequently display a sidedness in their manner of adhesion to epithelial surfaces. Other bacterial populations found on the tongue were normally members of chains and seldom present as single cells. This suggests that filamentous or chain-forming bacteria may have a selective advantage over single bacteria in their ability to colonize and remain attached to the epithelium of the tongue."}
{"id": "PMID:485147", "title": "Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh waters.", "content": "A membrane filter method (mA) for the enumeration of Aeromonas hydrophila in natural water samples was developed. The complex, primary medium employs trehalose as a fermentable carbohydrate and ampicillin and ethanol as selective inhibitors. After 20 h of incubation at 37 degrees C, an in situ mannitol fermentation test followed by an in situ oxidase test is used to further differentiate A. hydrophila from other aquatic and terrestrial microorganisms present in freshwaters. The primary medium decreases background microbial growth by about two orders of magnitude. The recoveries on mA medium from suspensions of A. hydrophila prepared from pure cultures and held for 24 h at 15 degrees C exceeded 95% of the recoveries on brain-heart infusion agar spread plates. The confirmation rate for colonies designated A. hydrophila was 98%, whereas 11% of the presumptively negative colonies were, in fact, A. hydrophila. Recoveries of A. hydrophila from fresh, surface water samples exceeded recoveries by the other methods examined.", "contents": "Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh waters. A membrane filter method (mA) for the enumeration of Aeromonas hydrophila in natural water samples was developed. The complex, primary medium employs trehalose as a fermentable carbohydrate and ampicillin and ethanol as selective inhibitors. After 20 h of incubation at 37 degrees C, an in situ mannitol fermentation test followed by an in situ oxidase test is used to further differentiate A. hydrophila from other aquatic and terrestrial microorganisms present in freshwaters. The primary medium decreases background microbial growth by about two orders of magnitude. The recoveries on mA medium from suspensions of A. hydrophila prepared from pure cultures and held for 24 h at 15 degrees C exceeded 95% of the recoveries on brain-heart infusion agar spread plates. The confirmation rate for colonies designated A. hydrophila was 98%, whereas 11% of the presumptively negative colonies were, in fact, A. hydrophila. Recoveries of A. hydrophila from fresh, surface water samples exceeded recoveries by the other methods examined."}
{"id": "PMID:485148", "title": "Protein-to-wet weight relationships in supragingival plaques from caries-prone tooth surfaces.", "content": "The ratio of protein to wet weight in unpooled samples of supragingival plaques from sound and carious tooth surfaces was studied. Protein was assayed by a procedure developed for the study, in order to achieve a sensitivity of 1 microgram with minimum effects upon quantitation from protein composition and nonprotein components. Ratios of protein to wet weight in plaque specimens from caries-free surfaces were almost equally distributed into two main categories of 9.4% and 6.5%. Corresponding values for specimens from carious surfaces were 9.1% and 5.0%. The occurrence of high and of low values among samples from each type of surface indicated that the plaques differed quantitatively in protein, water, or a nonprotein component, possibly extracellular polysaccharide. Although compositional differences between plaques from the two types of surfaces were indicated by the lower ratios of 6.5% from noncarious and 5.0% from carious surfaces, they were not indicated by the higher ratio values, which were similar. These results suggest either that protein-to-wet weight ratios are not related to caries, or that the ratio values are related to caries for some but not all types of plaques.", "contents": "Protein-to-wet weight relationships in supragingival plaques from caries-prone tooth surfaces. The ratio of protein to wet weight in unpooled samples of supragingival plaques from sound and carious tooth surfaces was studied. Protein was assayed by a procedure developed for the study, in order to achieve a sensitivity of 1 microgram with minimum effects upon quantitation from protein composition and nonprotein components. Ratios of protein to wet weight in plaque specimens from caries-free surfaces were almost equally distributed into two main categories of 9.4% and 6.5%. Corresponding values for specimens from carious surfaces were 9.1% and 5.0%. The occurrence of high and of low values among samples from each type of surface indicated that the plaques differed quantitatively in protein, water, or a nonprotein component, possibly extracellular polysaccharide. Although compositional differences between plaques from the two types of surfaces were indicated by the lower ratios of 6.5% from noncarious and 5.0% from carious surfaces, they were not indicated by the higher ratio values, which were similar. These results suggest either that protein-to-wet weight ratios are not related to caries, or that the ratio values are related to caries for some but not all types of plaques."}
{"id": "PMID:485149", "title": "Purification of an Endogenous polynucleotide phosphorylase from Brevibacterium JM98A.", "content": "Polynucleotide phosphorylase was purified form Brevibacterium JM98A (ATCC 29895). Homopolynucleotides were arsenolysed in the order polyadenylate greater than polycytidylic acid greater than polyuridylic acid greater than polyguanylate. The products were ribonucleoside 5'-monophosphates.", "contents": "Purification of an Endogenous polynucleotide phosphorylase from Brevibacterium JM98A. Polynucleotide phosphorylase was purified form Brevibacterium JM98A (ATCC 29895). Homopolynucleotides were arsenolysed in the order polyadenylate greater than polycytidylic acid greater than polyuridylic acid greater than polyguanylate. The products were ribonucleoside 5'-monophosphates."}
{"id": "PMID:485150", "title": "Modified lysine iron agar for isolation of Salmonella from food.", "content": "Lysine iron agar, modified by the addition of bile salts, novobiocin, lactose, and sucrose, is a valuable plating medium for the isolation of Salmonella, including H2S-negative strains.", "contents": "Modified lysine iron agar for isolation of Salmonella from food. Lysine iron agar, modified by the addition of bile salts, novobiocin, lactose, and sucrose, is a valuable plating medium for the isolation of Salmonella, including H2S-negative strains."}
{"id": "PMID:485151", "title": "Distribution of Aeromonas hydrophila in natural and man-made thermal effluents.", "content": "Densities of Aeromonas hydrophila showed distinct thermal optima (25 to 35 degrees C) and thermal maxima (45 degrees C) when measured along thermal gradients created by geothermal and nuclear reactor effluents. Survival of A. hydrophila never exceeded 48 h at temperatures of greater than 45 degrees C. Thermophilic strains could not be isolated at any site.", "contents": "Distribution of Aeromonas hydrophila in natural and man-made thermal effluents. Densities of Aeromonas hydrophila showed distinct thermal optima (25 to 35 degrees C) and thermal maxima (45 degrees C) when measured along thermal gradients created by geothermal and nuclear reactor effluents. Survival of A. hydrophila never exceeded 48 h at temperatures of greater than 45 degrees C. Thermophilic strains could not be isolated at any site."}
{"id": "PMID:485152", "title": "Exohemagglutinins: new products of vibrios.", "content": "A number of vibrio strains isolated from marine water produced high units of phytohemagglutinin-like agglutinins. Sugar specificity of the hemagglutinins was different from that of the sugar-binding bacterial toxins and that of the sugar-binding pili on the bacterial cell surfaces.", "contents": "Exohemagglutinins: new products of vibrios. A number of vibrio strains isolated from marine water produced high units of phytohemagglutinin-like agglutinins. Sugar specificity of the hemagglutinins was different from that of the sugar-binding bacterial toxins and that of the sugar-binding pili on the bacterial cell surfaces."}
{"id": "PMID:485153", "title": "Xylose, arabinose, and rhamnose fermentation by Bacteroides ruminicola.", "content": "Metabolism and growth yields of Bacteroides ruminicola grown on d-xylose, l-arabinose, and l-rhamnose were studied. Growth yields were 62, 68, and 35.5 g (dry weight) per mol of carbohydrate fermented after correction for storage polysaccharide. Experiments with [1-(14)C]arabinose indicated that pentose was fermented by a pentose phosphate cycle plus glycolysis, with some indication of a minor phosphoketolase-type pathway. The product ratios from pentose were similar to those previously described for hexose. Rhamnose was fermented mainly to 1,2-propanediol, succinate, and acetate, although the latter was quantitatively less than expected. Estimates of adenosine 5'-triphosphate (ATP) molar growth yields could not be calculated with any certainty, as ATP generation by electron transport-linked phosphorylation cannot yet be assessed. If ATP were generated by substrate-level phosphorylation reactions alone, ATP molar growth yields for xylose, arabinose, and rhamnose would be 30, 28, and 35 g/mol. If calculations are based on an assumption that two ATP are generated by electron transport-linked phosphorylation per succinate, ATP molar growth yields become 15, 14, and 22 g/mol; if the assumption is also made that the pathway of lactaldehyde reduction is coupled to production of one ATP per 1,2-propanediol by electron transport-linked phosphorylation, the ATP molar growth yield for rhamnose fermentation becomes 14 g/mol. No preference can be expressed between these alternatives at present.", "contents": "Xylose, arabinose, and rhamnose fermentation by Bacteroides ruminicola. Metabolism and growth yields of Bacteroides ruminicola grown on d-xylose, l-arabinose, and l-rhamnose were studied. Growth yields were 62, 68, and 35.5 g (dry weight) per mol of carbohydrate fermented after correction for storage polysaccharide. Experiments with [1-(14)C]arabinose indicated that pentose was fermented by a pentose phosphate cycle plus glycolysis, with some indication of a minor phosphoketolase-type pathway. The product ratios from pentose were similar to those previously described for hexose. Rhamnose was fermented mainly to 1,2-propanediol, succinate, and acetate, although the latter was quantitatively less than expected. Estimates of adenosine 5'-triphosphate (ATP) molar growth yields could not be calculated with any certainty, as ATP generation by electron transport-linked phosphorylation cannot yet be assessed. If ATP were generated by substrate-level phosphorylation reactions alone, ATP molar growth yields for xylose, arabinose, and rhamnose would be 30, 28, and 35 g/mol. If calculations are based on an assumption that two ATP are generated by electron transport-linked phosphorylation per succinate, ATP molar growth yields become 15, 14, and 22 g/mol; if the assumption is also made that the pathway of lactaldehyde reduction is coupled to production of one ATP per 1,2-propanediol by electron transport-linked phosphorylation, the ATP molar growth yield for rhamnose fermentation becomes 14 g/mol. No preference can be expressed between these alternatives at present."}
{"id": "PMID:485181", "title": "Elevated heparin-precipitable fraction of plasma in granuloma annulare.", "content": "The levels of heparin-precipitable fraction of plasma were determined in 17 patients with granuloma annulare and 26 persons used as controls. The mean level in patients was greater than the mean level in persons used as controls (P LESS THAN .05). Patients with widespread, disseminated lesions tended to have higher levels than patients with more localized disease (P LESS THAN .15). New lesions were actively forming in two patients with widespread disease at the time of study, and levels of heparin-precipitable fraction in these patients were among the highest found. The significance of these findings and their relationship to the etiopathogenesis of granuloma annulare is speculative.", "contents": "Elevated heparin-precipitable fraction of plasma in granuloma annulare. The levels of heparin-precipitable fraction of plasma were determined in 17 patients with granuloma annulare and 26 persons used as controls. The mean level in patients was greater than the mean level in persons used as controls (P LESS THAN .05). Patients with widespread, disseminated lesions tended to have higher levels than patients with more localized disease (P LESS THAN .15). New lesions were actively forming in two patients with widespread disease at the time of study, and levels of heparin-precipitable fraction in these patients were among the highest found. The significance of these findings and their relationship to the etiopathogenesis of granuloma annulare is speculative."}
{"id": "PMID:485182", "title": "Comparison of crude coal tar and topical methoxsalen in treatment of psoriasis.", "content": "Fluorescent sunlamp bulbs have been an effective light source for treatment of psoriasis when they are used in combination with crude coal tar. In addition to their ultraviolet B (UVB) emission, the spectral output of these bulbs contains a substantial amount of ultraviolet A (UVA). Prior testing with this light source and topically applied methoxsalen achieved excellent results in psoriasis. This study compared topically applied methoxsalen to crude coal tar in 16 patients who had plaque-type psoriasis, using the same fluorescent sunlamp source of irradiation. Fourteen of 16 patients had complete clearing of plaques when they were treated with methoxsalen, compared with six patients who had complete clearing with the tar treatment. These results indicate that the use of methoxsalen and ultraviolet light may be more effective than tar, when used as they were in this study. The advantages of a clean, white, nonstaining topical agent also makes outpatient therapy more cosmetically acceptable.", "contents": "Comparison of crude coal tar and topical methoxsalen in treatment of psoriasis. Fluorescent sunlamp bulbs have been an effective light source for treatment of psoriasis when they are used in combination with crude coal tar. In addition to their ultraviolet B (UVB) emission, the spectral output of these bulbs contains a substantial amount of ultraviolet A (UVA). Prior testing with this light source and topically applied methoxsalen achieved excellent results in psoriasis. This study compared topically applied methoxsalen to crude coal tar in 16 patients who had plaque-type psoriasis, using the same fluorescent sunlamp source of irradiation. Fourteen of 16 patients had complete clearing of plaques when they were treated with methoxsalen, compared with six patients who had complete clearing with the tar treatment. These results indicate that the use of methoxsalen and ultraviolet light may be more effective than tar, when used as they were in this study. The advantages of a clean, white, nonstaining topical agent also makes outpatient therapy more cosmetically acceptable."}
{"id": "PMID:485183", "title": "Evans blue dermatitis.", "content": "Contact dermatitis developed in five patients due to Evans blue, an azo-type dye used ot outline the lymphatics prior to the injection of a contrast medium in performing lymphangiography. A pathophysiologic mechanism for the development of dermatitis due to Evans blue is proposed.", "contents": "Evans blue dermatitis. Contact dermatitis developed in five patients due to Evans blue, an azo-type dye used ot outline the lymphatics prior to the injection of a contrast medium in performing lymphangiography. A pathophysiologic mechanism for the development of dermatitis due to Evans blue is proposed."}
{"id": "PMID:485184", "title": "Combination therapy for psoriasis. Psoralens plus long-wave ultraviolet radiation with betamethasone valerate.", "content": "In the treatment of psoriatic patients with psoralens plus long-wave ultraviolet radiation (PUVA), clearing of psoriatic lesions was obtained more quickly and with smaller doses of ultraviolet light when topically applied corticosteroid therapy was added. Twelve patients with symmetrical plaque-type psoriasis were given PUVA on one side of the body and PUVA plus betamethasone valerate on the other side in a paired comparison study. Ten of the patients had faster clearing of lesions on the side that was treated with PUVA and betamethasone than on the side treated with PUVA alone. The other two patients had equal clearing on both sides. All patients remained clear of lesions during maintenance with PUVA alone for at least five months after steroid therapy was discontinued. Combination therapy may save the patient time, expense, and unnecessary exposure to radiant energy.", "contents": "Combination therapy for psoriasis. Psoralens plus long-wave ultraviolet radiation with betamethasone valerate. In the treatment of psoriatic patients with psoralens plus long-wave ultraviolet radiation (PUVA), clearing of psoriatic lesions was obtained more quickly and with smaller doses of ultraviolet light when topically applied corticosteroid therapy was added. Twelve patients with symmetrical plaque-type psoriasis were given PUVA on one side of the body and PUVA plus betamethasone valerate on the other side in a paired comparison study. Ten of the patients had faster clearing of lesions on the side that was treated with PUVA and betamethasone than on the side treated with PUVA alone. The other two patients had equal clearing on both sides. All patients remained clear of lesions during maintenance with PUVA alone for at least five months after steroid therapy was discontinued. Combination therapy may save the patient time, expense, and unnecessary exposure to radiant energy."}
{"id": "PMID:485185", "title": "Actinic reticuloid. A clinical, pathologic, and action spectrum study.", "content": "A 54-year-old man suffered from a chronic dermatitis that was more severe on light-exposed areas. Skin biopsy specimens revealed histopathologic findings consistent with those seen in mycosis fungoides. Ultrastructural studies demonstrated the existence of lymphoid cells with hyperconvoluted nuclei, typical of the mycosis fungoides cell or S\u00e9zary cell, within the epidermis, dermis, and peripheral blood. Light testing with a monochromator showed abnormal photosensitivity to both short- and long-wave ultraviolet light as well as to the violet, blue, and green wavelengths of visible light. The features of this patient's disease indicate actinic reticuloid.", "contents": "Actinic reticuloid. A clinical, pathologic, and action spectrum study. A 54-year-old man suffered from a chronic dermatitis that was more severe on light-exposed areas. Skin biopsy specimens revealed histopathologic findings consistent with those seen in mycosis fungoides. Ultrastructural studies demonstrated the existence of lymphoid cells with hyperconvoluted nuclei, typical of the mycosis fungoides cell or S\u00e9zary cell, within the epidermis, dermis, and peripheral blood. Light testing with a monochromator showed abnormal photosensitivity to both short- and long-wave ultraviolet light as well as to the violet, blue, and green wavelengths of visible light. The features of this patient's disease indicate actinic reticuloid."}
{"id": "PMID:485186", "title": "Median raphe cysts of the penis.", "content": "The occurrence of a ventral cystic lesion of the penis should alert the clinician to the diagnosis of a median raphe cyst. The lesions, which are most common near the glans penis, may occur anywhere from the urethral meatus to the anus. Cysts of the median raphe represent defects in the embryologic development of the genitalia and are usually lined by entodermal epithelium. Surgical excision is the treatment of choice.", "contents": "Median raphe cysts of the penis. The occurrence of a ventral cystic lesion of the penis should alert the clinician to the diagnosis of a median raphe cyst. The lesions, which are most common near the glans penis, may occur anywhere from the urethral meatus to the anus. Cysts of the median raphe represent defects in the embryologic development of the genitalia and are usually lined by entodermal epithelium. Surgical excision is the treatment of choice."}
{"id": "PMID:485187", "title": "Pigmented lesion following complete removal of melanoma.", "content": "A pigmented lesion developed in three patients in the center of the scar following excision and grafting to remove melanoma. Histologically, the lesions showed hyperplasia of melanocytes resembling junction nevus or lentigo maligna. Some site-specific factor seems to be responsible for this change.", "contents": "Pigmented lesion following complete removal of melanoma. A pigmented lesion developed in three patients in the center of the scar following excision and grafting to remove melanoma. Histologically, the lesions showed hyperplasia of melanocytes resembling junction nevus or lentigo maligna. Some site-specific factor seems to be responsible for this change."}
{"id": "PMID:485188", "title": "Nodular nonsuppurative panniculitis associated with jejunoileal bypass surgery.", "content": "A 32-year-old woman underwent jejunoileal bypass surgery for morbid obesity. Her postoperative course was marked with many of the complications of this type of surgery. In addition, tender, erythematous nodules developed on her legs and abdomen that were grossly and microscopically consistent with nodular nonsuppurative panniculitis. She did not have any condition or disease previously reported to be associated with nodular nonsuppurative inflammation. The nodular panniculitis in this patient appears to be a new complication of small-bowel bypass surgery.", "contents": "Nodular nonsuppurative panniculitis associated with jejunoileal bypass surgery. A 32-year-old woman underwent jejunoileal bypass surgery for morbid obesity. Her postoperative course was marked with many of the complications of this type of surgery. In addition, tender, erythematous nodules developed on her legs and abdomen that were grossly and microscopically consistent with nodular nonsuppurative panniculitis. She did not have any condition or disease previously reported to be associated with nodular nonsuppurative inflammation. The nodular panniculitis in this patient appears to be a new complication of small-bowel bypass surgery."}
{"id": "PMID:485189", "title": "Cutaneous manifestations of tumoral calcinosis.", "content": "We have followed up a large family in which seven members have tumoral calcinosis. One girl had the skin lesions of localized calcinosis cutis apart from the typical subcutaneous deposits of calcium. Like most persons with tumoral calcinosis, our patient had normal serum calcium concentrations; however, the serum phosphorus levels were greatly elevated. The familial occurrence and elevated serum phosphorus levels suggest the possibility of some as yet undefined, heritable metabolic defect as the underlying cause. The occurrence of tumoral calcinosis with localized calcinosis cutis is a rare association, and there has been only one other reported case to our knowledge. This report describes our patient and offers a brief discussion of tumoral calcinosis. The therapeutic response to the phosphate depletion regimen and topical steroids was disappointing in our case.", "contents": "Cutaneous manifestations of tumoral calcinosis. We have followed up a large family in which seven members have tumoral calcinosis. One girl had the skin lesions of localized calcinosis cutis apart from the typical subcutaneous deposits of calcium. Like most persons with tumoral calcinosis, our patient had normal serum calcium concentrations; however, the serum phosphorus levels were greatly elevated. The familial occurrence and elevated serum phosphorus levels suggest the possibility of some as yet undefined, heritable metabolic defect as the underlying cause. The occurrence of tumoral calcinosis with localized calcinosis cutis is a rare association, and there has been only one other reported case to our knowledge. This report describes our patient and offers a brief discussion of tumoral calcinosis. The therapeutic response to the phosphate depletion regimen and topical steroids was disappointing in our case."}
{"id": "PMID:485193", "title": "Mycoplasma pneuminia infection. A follow-up study of 50 children with respiratory illness.", "content": "Fifty children with a previous history of Mycoplasma pneumoniae respiratory tract infection were assessed clinically, and pulmonary function tests carried out after an interval ranging from 1 1/2 to 9 1/2 years (median 2 1/2). 23 suffered from recurrent wheezy bronchitis or asthma, and in 5 the index illness appeared to precipitate the wheezing tendency. All were symptom-free when respiratory function tests were performed. Simple tests of ventilatory function (PEFR, FEV, and FVC) were within normal limits. Increased bronchial reactivity after exercise (a fall in PEFR greater than 15% resting value) was demonstrated only in children known to have asthma. Maximum expiratory flow rates in air at 50% of vital capacity (V mas50) were within the normal range in all patients with the exception of two. The response in flow rate at 50% of vital capacity after inalation of an 80% helium and 20% oxygen mixture delta V max50) was reduced (P less than 0.001) in asymptomatic patients with a history of M. pneumoniae respiratory infection, when compared with normal data from 48 healthy schoolchildren without a background of significant respiratory illnesses. These findings indicate impairment of small airways function, even in totally symptom-free children in the study group.", "contents": "Mycoplasma pneuminia infection. A follow-up study of 50 children with respiratory illness. Fifty children with a previous history of Mycoplasma pneumoniae respiratory tract infection were assessed clinically, and pulmonary function tests carried out after an interval ranging from 1 1/2 to 9 1/2 years (median 2 1/2). 23 suffered from recurrent wheezy bronchitis or asthma, and in 5 the index illness appeared to precipitate the wheezing tendency. All were symptom-free when respiratory function tests were performed. Simple tests of ventilatory function (PEFR, FEV, and FVC) were within normal limits. Increased bronchial reactivity after exercise (a fall in PEFR greater than 15% resting value) was demonstrated only in children known to have asthma. Maximum expiratory flow rates in air at 50% of vital capacity (V mas50) were within the normal range in all patients with the exception of two. The response in flow rate at 50% of vital capacity after inalation of an 80% helium and 20% oxygen mixture delta V max50) was reduced (P less than 0.001) in asymptomatic patients with a history of M. pneumoniae respiratory infection, when compared with normal data from 48 healthy schoolchildren without a background of significant respiratory illnesses. These findings indicate impairment of small airways function, even in totally symptom-free children in the study group."}
{"id": "PMID:485194", "title": "Effects of physical training on hormonal responses to exercise in asthmatic children.", "content": "A 4-month period of physical training increased the physical working capacity of 16 asthmatic children (aged between 9.3 and 13.6 years) by a mean of 11%. The increase was greater in boys and was negatively correlated with pretraining capacity. Urinary excretion of adrenaline, measured during a submaximal exercise test, decreased during the training period; the decrease was correlated with the increase in working capacity. Be fore the training period, exercise induced an increase in the plasma cortisol level; no increase was evident after training. In contrast, the training did not affect the exercise-induced increase in the plasma growth hormone level.", "contents": "Effects of physical training on hormonal responses to exercise in asthmatic children. A 4-month period of physical training increased the physical working capacity of 16 asthmatic children (aged between 9.3 and 13.6 years) by a mean of 11%. The increase was greater in boys and was negatively correlated with pretraining capacity. Urinary excretion of adrenaline, measured during a submaximal exercise test, decreased during the training period; the decrease was correlated with the increase in working capacity. Be fore the training period, exercise induced an increase in the plasma cortisol level; no increase was evident after training. In contrast, the training did not affect the exercise-induced increase in the plasma growth hormone level."}
{"id": "PMID:485195", "title": "Dublin-Johnson syndrome with some unusual features in a Chinese family.", "content": "Three cases of chronic nonhaemolytic jaundice with conjugated bilirubin in the serum are described in a Chinese family. Bromsulphthalein excretion tests gave results typical of the Dubin-Johnson syndrome. Liver histology in the proband showed cytoplasmic pigment of the lipofuscinmelanin variety, and intravenous cholecystography failed to show visualisation of the gallbladder. Unusual findings included onset during the neonatal period in the proband and the presence of some iron pigment in the hepatic cells with a little canalicular cholestasis. It is suggested that the infant may have had a concomitant nonspecific hepatitis. These cases are regarded as belonging to a disease group in which the Dubin-Johnson syndrome is at one end of a spectrum. The mode of inheritance is discussed.", "contents": "Dublin-Johnson syndrome with some unusual features in a Chinese family. Three cases of chronic nonhaemolytic jaundice with conjugated bilirubin in the serum are described in a Chinese family. Bromsulphthalein excretion tests gave results typical of the Dubin-Johnson syndrome. Liver histology in the proband showed cytoplasmic pigment of the lipofuscinmelanin variety, and intravenous cholecystography failed to show visualisation of the gallbladder. Unusual findings included onset during the neonatal period in the proband and the presence of some iron pigment in the hepatic cells with a little canalicular cholestasis. It is suggested that the infant may have had a concomitant nonspecific hepatitis. These cases are regarded as belonging to a disease group in which the Dubin-Johnson syndrome is at one end of a spectrum. The mode of inheritance is discussed."}
{"id": "PMID:485196", "title": "Carrier detection in Duchenne muscular dystrophy. Evidence from a study of obligatory carriers and mothers of isolated cases.", "content": "The mean levels of serum creatinine phosphokinase (CPK) were studied in three groups of women: normal controls (57), obligate carriers for Duchenne muscular dystrophy (30), and mothers of isolated cases of this disease (35). The distribution of the levels in these groups was significantly different and was in keeping with the hypothesis that one-third of isolated cases result from new mutations. The control and carrier ranges overlapped considerably, with the level of CPK of 33% of obligate carriers coming within the 97 1/2 centile of the normal range. Odds against an individual being a carrier were derived for specific mean values of CPK. They should be considered with genetic information using Bayes's theorem. The mean CPK levels in obligate carriers showed significant familial clustering. This may have implications in carrier detection.", "contents": "Carrier detection in Duchenne muscular dystrophy. Evidence from a study of obligatory carriers and mothers of isolated cases. The mean levels of serum creatinine phosphokinase (CPK) were studied in three groups of women: normal controls (57), obligate carriers for Duchenne muscular dystrophy (30), and mothers of isolated cases of this disease (35). The distribution of the levels in these groups was significantly different and was in keeping with the hypothesis that one-third of isolated cases result from new mutations. The control and carrier ranges overlapped considerably, with the level of CPK of 33% of obligate carriers coming within the 97 1/2 centile of the normal range. Odds against an individual being a carrier were derived for specific mean values of CPK. They should be considered with genetic information using Bayes's theorem. The mean CPK levels in obligate carriers showed significant familial clustering. This may have implications in carrier detection."}
{"id": "PMID:485197", "title": "Serum C-reactive protein test in diagnosis of septic complications of cerebrospinal fluid shunts for hydrocephalus.", "content": "Serial tests for serum C-reactive protein (CRP) were carried out on 40 children undergoing cerebrospinal fluid shunt surgery, to determine the pattern of appearance and disappearance of CRP in relation to this type of operation, in the absence of postoperative complications. Samples of sera from a further 268 children, 79 of whom presented after shunt surgery with symptoms suggesting infective complications related to the shunt, were examined for CRP. The results showed that, while the test in negative in uncomplicated colonisation of ventriculo-atrial shunts, it is reliably positive in patients with colonised ventriculo-peritoneal shunts, ventriculitis, or shunt nephritis.", "contents": "Serum C-reactive protein test in diagnosis of septic complications of cerebrospinal fluid shunts for hydrocephalus. Serial tests for serum C-reactive protein (CRP) were carried out on 40 children undergoing cerebrospinal fluid shunt surgery, to determine the pattern of appearance and disappearance of CRP in relation to this type of operation, in the absence of postoperative complications. Samples of sera from a further 268 children, 79 of whom presented after shunt surgery with symptoms suggesting infective complications related to the shunt, were examined for CRP. The results showed that, while the test in negative in uncomplicated colonisation of ventriculo-atrial shunts, it is reliably positive in patients with colonised ventriculo-peritoneal shunts, ventriculitis, or shunt nephritis."}
{"id": "PMID:485198", "title": "Two cases of perinatal listeriosis.", "content": "Two cases of neonatal listeriosis are described. The incidence in the UK is given and the treatment of the pregnant woman is briefly discussed.", "contents": "Two cases of perinatal listeriosis. Two cases of neonatal listeriosis are described. The incidence in the UK is given and the treatment of the pregnant woman is briefly discussed."}
{"id": "PMID:485199", "title": "Water intoxication by the oral route in an infant.", "content": "Symptomatic water intoxication is common when hypo-osmolar fluids are given therapeutically, usually intravenously, but it is rare after drinking voluntarily (Wynn and Rob, 1954). We report a case of water intoxication caused by voluntary drinking in an infant.", "contents": "Water intoxication by the oral route in an infant. Symptomatic water intoxication is common when hypo-osmolar fluids are given therapeutically, usually intravenously, but it is rare after drinking voluntarily (Wynn and Rob, 1954). We report a case of water intoxication caused by voluntary drinking in an infant."}
{"id": "PMID:485200", "title": "Copper deficiency in a low birthweight infant.", "content": "Copper deficiency is reported in an infant of very low birthweight. It was characterised by extensive bone changes, severe neutropenia, and hypocupraemia. These manifestations could have been missed but for an intercurrent pneumonia which led to an x-ray of the chest.", "contents": "Copper deficiency in a low birthweight infant. Copper deficiency is reported in an infant of very low birthweight. It was characterised by extensive bone changes, severe neutropenia, and hypocupraemia. These manifestations could have been missed but for an intercurrent pneumonia which led to an x-ray of the chest."}
{"id": "PMID:485201", "title": "Pulmonary candidiasis in cystic fibrosis.", "content": "A child with cystic fibrosis and asthma developed pulmonary candidiasis. Predisposing factors in this patient were prolonged antibiotic therapy, high-dose corticosteroids, and intravenous catherisation. A diagnosis was made by lung puncture and confirmed by rapid response to 5-fluorocytosine.", "contents": "Pulmonary candidiasis in cystic fibrosis. A child with cystic fibrosis and asthma developed pulmonary candidiasis. Predisposing factors in this patient were prolonged antibiotic therapy, high-dose corticosteroids, and intravenous catherisation. A diagnosis was made by lung puncture and confirmed by rapid response to 5-fluorocytosine."}
{"id": "PMID:485202", "title": "Neonatal aspergillosis accompanying fulminant necrotising enterocolitis.", "content": "A preterm infant developed fulminant necrotising enterocolitis, subsequently resulting in death. Necropsy examination showed diffuse aspergillosis. Reports are reviewed, and possible mechanisms resulting in Aspergillus infection in this infant are discussed.", "contents": "Neonatal aspergillosis accompanying fulminant necrotising enterocolitis. A preterm infant developed fulminant necrotising enterocolitis, subsequently resulting in death. Necropsy examination showed diffuse aspergillosis. Reports are reviewed, and possible mechanisms resulting in Aspergillus infection in this infant are discussed."}
{"id": "PMID:485207", "title": "Toxicity of the herbicides 2,4-D, DEF, propanil and trifluralin to the Dungeness crab, Cancer magister.", "content": "Lethal and sublethal responses to the herbicides 2,4-D, DEF, propanil, and trifluralin of various life history stages of the Dungeness crab, Cancer magister, were examined to estimate maximum acceptable toxicant concentrations (MATC) of each compound for this species. Zoeae were found, in long term tests, to be the most sensitive stage. Based on the experiments with this stage, MATCs were concluded to be greater than 0.95, less than 6.9 microgram/L for DEF, greater than or equal to 26, less than 220 microgram/L for trifluralin, greater than or equal to 1,700 microgram/L for propanil, and less than 1,000 microgram/L for the free acid form of 2,4-D.", "contents": "Toxicity of the herbicides 2,4-D, DEF, propanil and trifluralin to the Dungeness crab, Cancer magister. Lethal and sublethal responses to the herbicides 2,4-D, DEF, propanil, and trifluralin of various life history stages of the Dungeness crab, Cancer magister, were examined to estimate maximum acceptable toxicant concentrations (MATC) of each compound for this species. Zoeae were found, in long term tests, to be the most sensitive stage. Based on the experiments with this stage, MATCs were concluded to be greater than 0.95, less than 6.9 microgram/L for DEF, greater than or equal to 26, less than 220 microgram/L for trifluralin, greater than or equal to 1,700 microgram/L for propanil, and less than 1,000 microgram/L for the free acid form of 2,4-D."}
{"id": "PMID:485208", "title": "Studies on the effect of dimecron on the digestive system of a fresh water fish, Channa punctatus.", "content": "The effect of exposure to a sublethal concentration (0.32 mg/L) of Dimecron for 20 days on the digestive system of a fresh water teleost fish, Channa punctatus has been studied. The most conspicuous pathological changes in the liver were vacuolation of the cytoplasm of hepatocytes, enlargement of nuclei, rupture of the cell membrane, liver cord disarray, damage of connective tissue, infiltration of phagocytes and necrosis. The mucosa of stomach was ruptured and the gastric glands were degenerated. The mucosal epithelium of intestine and pyloric caeca was also degenerated. The mucus secreting goblet cells showed hyperactivity and the intestinal lumen was filled with mucus. No conspicuous changes were noted in the rectum except for hypersecretion of mucus. Histochemical tests revealed that alkaline phosphatase and glucose-6-phosphatase were inhibited by Dimecron treatment. However, acid phosphatase showed slight increase in activity. Biochemical tests for enzyme activity confirmed the histochemical findings. Alkaline phosphatase activity was significantly inhibited in liver and intestine. There was a slight elevation in the activity of acid phosphatase in the liver and pyloric caeca of exposed fish, but this was not statistically significant. The glucose-6-phosphatase activity was significantly inhibited in the liver, intestine and pyloric caeca.", "contents": "Studies on the effect of dimecron on the digestive system of a fresh water fish, Channa punctatus. The effect of exposure to a sublethal concentration (0.32 mg/L) of Dimecron for 20 days on the digestive system of a fresh water teleost fish, Channa punctatus has been studied. The most conspicuous pathological changes in the liver were vacuolation of the cytoplasm of hepatocytes, enlargement of nuclei, rupture of the cell membrane, liver cord disarray, damage of connective tissue, infiltration of phagocytes and necrosis. The mucosa of stomach was ruptured and the gastric glands were degenerated. The mucosal epithelium of intestine and pyloric caeca was also degenerated. The mucus secreting goblet cells showed hyperactivity and the intestinal lumen was filled with mucus. No conspicuous changes were noted in the rectum except for hypersecretion of mucus. Histochemical tests revealed that alkaline phosphatase and glucose-6-phosphatase were inhibited by Dimecron treatment. However, acid phosphatase showed slight increase in activity. Biochemical tests for enzyme activity confirmed the histochemical findings. Alkaline phosphatase activity was significantly inhibited in liver and intestine. There was a slight elevation in the activity of acid phosphatase in the liver and pyloric caeca of exposed fish, but this was not statistically significant. The glucose-6-phosphatase activity was significantly inhibited in the liver, intestine and pyloric caeca."}
{"id": "PMID:485209", "title": "Absorption and elimination of 14C-cis-chlordane and 14C-photo-cis-chlordane by goldfish Caraccius auratus.", "content": "Photo-cis-chlordane is accumulated by goldfish to a greater extent than cis-chlordane during a 16-hr exposure to 5 parts per billion (ppb) of each insecticide. However, the concentration of photo-cis-chlordane in vital organs (brain, nervous system, heart, liver, kidneys) during the 1-, 2-, and 4-day exposure is less than that of cis-chlordane. Only the swim bladder shows a much higher concentration of photo-cis-chlordane than cis-chlordane. These differences could hardly account for twice the higher toxicity of the former to goldfish. The transfer of pre-exposed fish to insecticide-free water resulted in faster elimination of photo-cis-chlordane than cis-chlordane; after five weeks, about 90% of the former was eliminated as compared with only 58% of the latter. High residues of both insecticides in the bile and the intestine during the initial period of exposure indicate a biliary route of elimination.", "contents": "Absorption and elimination of 14C-cis-chlordane and 14C-photo-cis-chlordane by goldfish Caraccius auratus. Photo-cis-chlordane is accumulated by goldfish to a greater extent than cis-chlordane during a 16-hr exposure to 5 parts per billion (ppb) of each insecticide. However, the concentration of photo-cis-chlordane in vital organs (brain, nervous system, heart, liver, kidneys) during the 1-, 2-, and 4-day exposure is less than that of cis-chlordane. Only the swim bladder shows a much higher concentration of photo-cis-chlordane than cis-chlordane. These differences could hardly account for twice the higher toxicity of the former to goldfish. The transfer of pre-exposed fish to insecticide-free water resulted in faster elimination of photo-cis-chlordane than cis-chlordane; after five weeks, about 90% of the former was eliminated as compared with only 58% of the latter. High residues of both insecticides in the bile and the intestine during the initial period of exposure indicate a biliary route of elimination."}
{"id": "PMID:485210", "title": "Effects of inorganic lead on delta-aminolevulinic acid dehydratase activity and hematological variables in the rainbow trout, Salmo gairdnerii.", "content": "The activity of the enzyme delta-aminolevulinic acid dehydratase (ALA-D) was strongly depressed in erythrocytes, spleen and renal tissue of rainbow trouts (Salmo gairdnerii) exposed to sublethal levels of inorganic lead (10, 75 and 300 microgram Pb2+/L) for 30 days. The fish exposed to the highest lead concentration also showed an anemic response and basophilic stippling of erythrocytes. The lead exposure did not cause any alteration of the white blood cell picture. The ALA-D inhibition, the anemia and basophilic stippling seemed to persist in the lead-exposed fish even after a recovery period of seven weeks in lead-free water.", "contents": "Effects of inorganic lead on delta-aminolevulinic acid dehydratase activity and hematological variables in the rainbow trout, Salmo gairdnerii. The activity of the enzyme delta-aminolevulinic acid dehydratase (ALA-D) was strongly depressed in erythrocytes, spleen and renal tissue of rainbow trouts (Salmo gairdnerii) exposed to sublethal levels of inorganic lead (10, 75 and 300 microgram Pb2+/L) for 30 days. The fish exposed to the highest lead concentration also showed an anemic response and basophilic stippling of erythrocytes. The lead exposure did not cause any alteration of the white blood cell picture. The ALA-D inhibition, the anemia and basophilic stippling seemed to persist in the lead-exposed fish even after a recovery period of seven weeks in lead-free water."}
{"id": "PMID:485211", "title": "Exposure of field workers to organophosphorus insecticides: cotton.", "content": "The red blood cell and plasma cholinesterase (ChE) levels of cotton scouts inspecting foliage for insect infestation and damage were monitored. Group means for ChE activity were significantly depressed at one or more time(s) during four of the eight growing seasons studied. Although no symptoms of organophosphate poisoning were confirmed, several scouts exhibited ChE depressions over 50% of pre-exposure levels.", "contents": "Exposure of field workers to organophosphorus insecticides: cotton. The red blood cell and plasma cholinesterase (ChE) levels of cotton scouts inspecting foliage for insect infestation and damage were monitored. Group means for ChE activity were significantly depressed at one or more time(s) during four of the eight growing seasons studied. Although no symptoms of organophosphate poisoning were confirmed, several scouts exhibited ChE depressions over 50% of pre-exposure levels."}
{"id": "PMID:485212", "title": "Chlorinated phenoxyacetic acid derivatives and tetrachlorodibenzo-p-dioxin in foliage after application of 2,4,5-trichlorophenoxyacetic acid esters.", "content": "Six reforestation areas were sprayed with 2,4,5-T esters. Leaf samples from the areas were analyzed for the esters, tetrachlorodioxin, and dehalogenated phenoxyacetic acid esters. Tetrachlorodioxin was found in one leaf sample only, but the level found, relative to the amount of 2,4,5-T ester found, was lower than in the formulation material used in the study. The concentration of the dichlorophenoxyacetic acid esters present in several leaf samples was greater, relative to the 2,4,5-T ester, which indicated a photochemical breakdown of the 2,4,5-T in the environment.", "contents": "Chlorinated phenoxyacetic acid derivatives and tetrachlorodibenzo-p-dioxin in foliage after application of 2,4,5-trichlorophenoxyacetic acid esters. Six reforestation areas were sprayed with 2,4,5-T esters. Leaf samples from the areas were analyzed for the esters, tetrachlorodioxin, and dehalogenated phenoxyacetic acid esters. Tetrachlorodioxin was found in one leaf sample only, but the level found, relative to the amount of 2,4,5-T ester found, was lower than in the formulation material used in the study. The concentration of the dichlorophenoxyacetic acid esters present in several leaf samples was greater, relative to the 2,4,5-T ester, which indicated a photochemical breakdown of the 2,4,5-T in the environment."}
{"id": "PMID:485213", "title": "Effects of chronic cadmium exposure on crayfish survival, growth, and tolerance to elevated temperatures.", "content": "Crayfish Cambarus latimanus were subjected over a 5-month period to 0.02, 5, and 10 microgram Cd/L in a continuous flow system at ambient winter temperatures. Uptake of cadmium, growth, and mortality were measured. One-half of the surviving crayfish were analyzed for Cd after completion of the 5-month experiment, while the remaining half were removed from the contaminated water and subjected in a static laboratory experiment to stepwise, daily increases in temperature. The results indicated that (1) the rate of Cd uptake in crayfish was positively related to external concentration, (2) mortality increased significantly with higher Cd concentrations, and (3) neither growth nor thermal tolerance was significantly related to the Cd concentration in the crayfish tissues. The importance of chronic, low-level studies is discussed.", "contents": "Effects of chronic cadmium exposure on crayfish survival, growth, and tolerance to elevated temperatures. Crayfish Cambarus latimanus were subjected over a 5-month period to 0.02, 5, and 10 microgram Cd/L in a continuous flow system at ambient winter temperatures. Uptake of cadmium, growth, and mortality were measured. One-half of the surviving crayfish were analyzed for Cd after completion of the 5-month experiment, while the remaining half were removed from the contaminated water and subjected in a static laboratory experiment to stepwise, daily increases in temperature. The results indicated that (1) the rate of Cd uptake in crayfish was positively related to external concentration, (2) mortality increased significantly with higher Cd concentrations, and (3) neither growth nor thermal tolerance was significantly related to the Cd concentration in the crayfish tissues. The importance of chronic, low-level studies is discussed."}
{"id": "PMID:485214", "title": "Reproductive responses of quail to bunker C oil fractions.", "content": "Bunker C fuel oil was extracted with petroleum ether and the concentrated extract was given to breeding Japanese quail (Coturnix coturnix Japonica). A dose of extract equivalent to 300 mg bunker C reduced both egg production and hatchability. Fractionation of the extract by absorption on Florisil and elution with petroleum ether followed by chloroform yielded fractions that were tested for toxicity. All the fractions had a pronounced effect upon production; however, only the chloroform eluates were found to affect hatchability of the eggs.", "contents": "Reproductive responses of quail to bunker C oil fractions. Bunker C fuel oil was extracted with petroleum ether and the concentrated extract was given to breeding Japanese quail (Coturnix coturnix Japonica). A dose of extract equivalent to 300 mg bunker C reduced both egg production and hatchability. Fractionation of the extract by absorption on Florisil and elution with petroleum ether followed by chloroform yielded fractions that were tested for toxicity. All the fractions had a pronounced effect upon production; however, only the chloroform eluates were found to affect hatchability of the eggs."}
{"id": "PMID:485215", "title": "Developmental and methylmercury effects on brain protein synthesis.", "content": "Sprague-Dawley rats (ages 1 to 21 days, alternate days) were chosen for study. Animals were injected with methylmercuric chloride (8 mg/kg IP); 12 hr later each animal was injected with uniformly labeled L-(14C)leucine, allowed 30 min for incorporation, and decapitated. Brains were analyzed for amounts of radioactivity incorporated into TCA precipitable protein. When compared to baseline curves for control animals, curves of treated animals were compressed overall and had age-dependent increases and decreases in rates of protein synthesis.", "contents": "Developmental and methylmercury effects on brain protein synthesis. Sprague-Dawley rats (ages 1 to 21 days, alternate days) were chosen for study. Animals were injected with methylmercuric chloride (8 mg/kg IP); 12 hr later each animal was injected with uniformly labeled L-(14C)leucine, allowed 30 min for incorporation, and decapitated. Brains were analyzed for amounts of radioactivity incorporated into TCA precipitable protein. When compared to baseline curves for control animals, curves of treated animals were compressed overall and had age-dependent increases and decreases in rates of protein synthesis."}
{"id": "PMID:485216", "title": "Gel and anion exchange chromatographic properties of copper-containing metallothioneins.", "content": "Chromatographic properties of copper-binding proteins (copper-containing metallothioneins) induced in rat liver and kidney by the injection of either copper in the liver and kidney or cadmium in the kidney were compared with those of metallothioneins, with low or no copper content, induced by the injection of cadmium or zinc in the liver both on a Sephadex G-75 column and on a DEAE Sephadex A-25 column. The copper-containing metallothioneins were eluted at a slightly slower rate on a Sephadex G-75 column and at a higher buffer concentration on an anion exchange column than the metallothioneins with low or no copper content regardless of the inducing metals, copper or cadmium. The different chromatographic properties of the metallothioneins with high and low copper contents may be explained by the more compact conformation of the proteins with high copper contents than those with low or no copper contents and by the changes of electrostatic charge with coordination by copper.", "contents": "Gel and anion exchange chromatographic properties of copper-containing metallothioneins. Chromatographic properties of copper-binding proteins (copper-containing metallothioneins) induced in rat liver and kidney by the injection of either copper in the liver and kidney or cadmium in the kidney were compared with those of metallothioneins, with low or no copper content, induced by the injection of cadmium or zinc in the liver both on a Sephadex G-75 column and on a DEAE Sephadex A-25 column. The copper-containing metallothioneins were eluted at a slightly slower rate on a Sephadex G-75 column and at a higher buffer concentration on an anion exchange column than the metallothioneins with low or no copper content regardless of the inducing metals, copper or cadmium. The different chromatographic properties of the metallothioneins with high and low copper contents may be explained by the more compact conformation of the proteins with high copper contents than those with low or no copper contents and by the changes of electrostatic charge with coordination by copper."}
{"id": "PMID:485218", "title": "Studies on the development of cell contacts and of the intercellular matrix during decidualization in the rat.", "content": "Cell contacts between developing decidual cells were examined in thin sections and in replicas of freeze-cleaved tissue of rat uterus. At the time of implantation only puncta adhaerentia were found between stromal cells. Gap junctions appeared 24 h after decidualization and thereafter increased in number and extent. Thus, there is little morphological evidence for a propagation of the initial stimulus for decidualization via gap junctions. An increase in amorphous, extracellular material is noted after decidualization with a concomitant decrease of collagen fibers. This amorphous material probably represents fibrinoid.", "contents": "Studies on the development of cell contacts and of the intercellular matrix during decidualization in the rat. Cell contacts between developing decidual cells were examined in thin sections and in replicas of freeze-cleaved tissue of rat uterus. At the time of implantation only puncta adhaerentia were found between stromal cells. Gap junctions appeared 24 h after decidualization and thereafter increased in number and extent. Thus, there is little morphological evidence for a propagation of the initial stimulus for decidualization via gap junctions. An increase in amorphous, extracellular material is noted after decidualization with a concomitant decrease of collagen fibers. This amorphous material probably represents fibrinoid."}
{"id": "PMID:485219", "title": "Pregnancy in a patient with Raynaud's disease.", "content": "We report a patient who, at the time of her third pregnancy at the age of 35, had had Raynaud's disease for 18 years. Her first pregnancy (during which she took Marcumar, an anticoagulant) ended in a miscarriage at three months gestation. The second pregnancy ended in fetal death due to placental insufficiency. The third pregnancy was also complicated by placental insufficiency which became evident during the second trimester. The patient was observed carefully and allowed to continue to 37 weeks gestation when a Caesarean section was done for late fetal heart rate decelerations during early labour of spontaneous onset. The baby had a low birth weight but developed normally. The placenta showed certain abnormalities which are described.", "contents": "Pregnancy in a patient with Raynaud's disease. We report a patient who, at the time of her third pregnancy at the age of 35, had had Raynaud's disease for 18 years. Her first pregnancy (during which she took Marcumar, an anticoagulant) ended in a miscarriage at three months gestation. The second pregnancy ended in fetal death due to placental insufficiency. The third pregnancy was also complicated by placental insufficiency which became evident during the second trimester. The patient was observed carefully and allowed to continue to 37 weeks gestation when a Caesarean section was done for late fetal heart rate decelerations during early labour of spontaneous onset. The baby had a low birth weight but developed normally. The placenta showed certain abnormalities which are described."}
{"id": "PMID:485220", "title": "[Isolation and characterization of a new placenta specific protein (PP10) (author's transl)].", "content": "PP10 was isolated from aqueous extracts of human term placentae by fractionating the proteins with rivanol and ammonium sulfate, by gelfiltration on Sephadex G-150 and by use of immunoadsorbents. PP10 apparently is a protein specific for the placenta; it could not be detected in extracts from other human tissues. From one human term placenta an average amount of 20 mg PP10 can be extracted. In sera from pregnant women PP10 is usually present only in trace amounts (less than 0.1 mg/100 ml). PP10 has the electrophoretic mobility of an alpha1-globulin and an isoelectric point of 5.1. The purified protein sediments with 3.8 S. PP10 was found to have a molecular weight of 48,000 as determined by ultracentrifugation and a molecular weight of 65,000 as determined by SDS-PAA gel electrophoresis. PP10 is a glycoprotein containing 6.65% carbohydrates (hexoses 4.8%, hexosamines 1.2%, fucose 0.05%, sialic acid 0.6%). The amino acid composition of PP10 has been determined, too; the most abundant amino acids in this protein are glutamic acid, aspartic acid, leucine and alanine.", "contents": "[Isolation and characterization of a new placenta specific protein (PP10) (author's transl)]. PP10 was isolated from aqueous extracts of human term placentae by fractionating the proteins with rivanol and ammonium sulfate, by gelfiltration on Sephadex G-150 and by use of immunoadsorbents. PP10 apparently is a protein specific for the placenta; it could not be detected in extracts from other human tissues. From one human term placenta an average amount of 20 mg PP10 can be extracted. In sera from pregnant women PP10 is usually present only in trace amounts (less than 0.1 mg/100 ml). PP10 has the electrophoretic mobility of an alpha1-globulin and an isoelectric point of 5.1. The purified protein sediments with 3.8 S. PP10 was found to have a molecular weight of 48,000 as determined by ultracentrifugation and a molecular weight of 65,000 as determined by SDS-PAA gel electrophoresis. PP10 is a glycoprotein containing 6.65% carbohydrates (hexoses 4.8%, hexosamines 1.2%, fucose 0.05%, sialic acid 0.6%). The amino acid composition of PP10 has been determined, too; the most abundant amino acids in this protein are glutamic acid, aspartic acid, leucine and alanine."}
{"id": "PMID:485221", "title": "IgG levels in maternal and umbilical cord serum after vaginal delivery and after elective Caesarean section.", "content": "Concentrations of IgG is maternal serum and in umbilical cord blood were determined in two groups of patients: one group had spontaneous vaginal deliveries and the other elective Caesarean sections. Mean umbilical cord serum IgG after vaginal delivery was significantly higher than the mean value after elective Caesarean section. The difference between the maternal serum IgG livel and the umbilical cord serum IgG level increased as did the duration of labour.", "contents": "IgG levels in maternal and umbilical cord serum after vaginal delivery and after elective Caesarean section. Concentrations of IgG is maternal serum and in umbilical cord blood were determined in two groups of patients: one group had spontaneous vaginal deliveries and the other elective Caesarean sections. Mean umbilical cord serum IgG after vaginal delivery was significantly higher than the mean value after elective Caesarean section. The difference between the maternal serum IgG livel and the umbilical cord serum IgG level increased as did the duration of labour."}
{"id": "PMID:485222", "title": "Trial of the calcium antagonist nifedipine in the treatment of primary dysmenorrhoea.", "content": "The effect of the calcium antagonist, nifedipine, on menstrual pain was investigated in 40 women with severe, primary dysmenorrhoea and 36 of them were observed over 3 consecutive menstrual cycles. Twenty-six patients experienced good pain relief, 10 moderate relief and 4 reported no benefit. The frequency of symptoms associated with menstrual pain was not reduced. Fifteen women regularly suffering from migraine during the menstrual period reported increased headache after intake of the drug. Due to this side effect four of these patients did not continue treatment for more than one cycle. All patients had transient facial flushing occurring 15--30 min after drug intake; this was well tolerated. An increase in pulse rate was also invariably found. However, only 5 patients complained of palpitations. Twenty-five of the 36 women completing the three-month trial wanted to continue nifedipine therapy regularly. It is concluded that calcium antagonists like nifedipine can be used for treatment of severe primary dysmenorrhoea, and that further evaluations of these drugs are indicated.", "contents": "Trial of the calcium antagonist nifedipine in the treatment of primary dysmenorrhoea. The effect of the calcium antagonist, nifedipine, on menstrual pain was investigated in 40 women with severe, primary dysmenorrhoea and 36 of them were observed over 3 consecutive menstrual cycles. Twenty-six patients experienced good pain relief, 10 moderate relief and 4 reported no benefit. The frequency of symptoms associated with menstrual pain was not reduced. Fifteen women regularly suffering from migraine during the menstrual period reported increased headache after intake of the drug. Due to this side effect four of these patients did not continue treatment for more than one cycle. All patients had transient facial flushing occurring 15--30 min after drug intake; this was well tolerated. An increase in pulse rate was also invariably found. However, only 5 patients complained of palpitations. Twenty-five of the 36 women completing the three-month trial wanted to continue nifedipine therapy regularly. It is concluded that calcium antagonists like nifedipine can be used for treatment of severe primary dysmenorrhoea, and that further evaluations of these drugs are indicated."}
{"id": "PMID:485223", "title": "Morphologic survey of the condylomatous lesions in dysplastic and neoplastic epithelium of the uterine cervix.", "content": "One hundred eighty-four women with a histologically verified dysplastic or neoplastic lesion in the uterine cervical epithelium were histologically assessed with special reference to the presence of the various histological types of condyloma in these epithelial specimens. Histological changes fulfilling the previously outlined criteria of the flat, inverted, and papillomatous condylomas were encountered in 91 (49.4%) women. Condylomatous lesions were seen in association with all degrees of epithelial atypia from mild dysplasia to frankly invasive cervical carcinoma, which seemed to develop at significantly earlier age when concomitant condylomatous lesion was present than with its absence (p less than 0.001). The serious consideration of the relationship between the condylomatous lesions and the uterine cervical was emphasized.", "contents": "Morphologic survey of the condylomatous lesions in dysplastic and neoplastic epithelium of the uterine cervix. One hundred eighty-four women with a histologically verified dysplastic or neoplastic lesion in the uterine cervical epithelium were histologically assessed with special reference to the presence of the various histological types of condyloma in these epithelial specimens. Histological changes fulfilling the previously outlined criteria of the flat, inverted, and papillomatous condylomas were encountered in 91 (49.4%) women. Condylomatous lesions were seen in association with all degrees of epithelial atypia from mild dysplasia to frankly invasive cervical carcinoma, which seemed to develop at significantly earlier age when concomitant condylomatous lesion was present than with its absence (p less than 0.001). The serious consideration of the relationship between the condylomatous lesions and the uterine cervical was emphasized."}
{"id": "PMID:485224", "title": "Factor XIII levels in relation to gynaecological operations.", "content": "In 25 patients with vaginal hysterectomies and 14 with abdominal hysterectomies, Factor XIII activity in the antiseruminhibition test of Bohn and Haupt was measured in the first 7 postoperative days. Both groups showed a statistically significant reduction in activity (40--50%) which started at 2 h after operation and lasted until the 3rd postoperative day. The route of operation did not significantly affect the results and neither did the phase of the cycle nor the age of the patient.", "contents": "Factor XIII levels in relation to gynaecological operations. In 25 patients with vaginal hysterectomies and 14 with abdominal hysterectomies, Factor XIII activity in the antiseruminhibition test of Bohn and Haupt was measured in the first 7 postoperative days. Both groups showed a statistically significant reduction in activity (40--50%) which started at 2 h after operation and lasted until the 3rd postoperative day. The route of operation did not significantly affect the results and neither did the phase of the cycle nor the age of the patient."}
{"id": "PMID:485225", "title": "The use of prostaglandin gel in obstetrics and gynecology.", "content": "The use of prostaglandin gel for therapeutic termination of second trimester pregnancy, the management of intrauterine fetal death, ripening of the cervix and induction of labour is reviewed. For these indications, prostaglandin gel is acceptable to patients, effective, and has low incidence of side effects and complications.", "contents": "The use of prostaglandin gel in obstetrics and gynecology. The use of prostaglandin gel for therapeutic termination of second trimester pregnancy, the management of intrauterine fetal death, ripening of the cervix and induction of labour is reviewed. For these indications, prostaglandin gel is acceptable to patients, effective, and has low incidence of side effects and complications."}
{"id": "PMID:485226", "title": "Limits of therapy for malignant chorioepithelioma.", "content": "Report on two cases of malignant chorio-epithelioma following hydatidiform mole, both decreased 4 years after inception of the disease despite initially successful therapy and repeated remissions. In the first case a solitary pulmonary metastasis showing obvious de-differentiation and extensive local reparative processes was removed operatively. Associations to cytostatic medication are discussed. The second case was characterized by unusually extensive chronic pulmonary embolism with highly regressive choriocardinomatous aggregates. Death resulted unexpectedly from chronic dextrocardial insufficiency. It is stressed that the treatment is subject ot limitations of not only relative (i.e., resistance to cytostatics, inadequate treatment etc.) but also of an absolute nature, as in case 2.", "contents": "Limits of therapy for malignant chorioepithelioma. Report on two cases of malignant chorio-epithelioma following hydatidiform mole, both decreased 4 years after inception of the disease despite initially successful therapy and repeated remissions. In the first case a solitary pulmonary metastasis showing obvious de-differentiation and extensive local reparative processes was removed operatively. Associations to cytostatic medication are discussed. The second case was characterized by unusually extensive chronic pulmonary embolism with highly regressive choriocardinomatous aggregates. Death resulted unexpectedly from chronic dextrocardial insufficiency. It is stressed that the treatment is subject ot limitations of not only relative (i.e., resistance to cytostatics, inadequate treatment etc.) but also of an absolute nature, as in case 2."}
{"id": "PMID:485227", "title": "Sexual differentiation of the hypothalamus in gonadal agenesis and testicular feminization.", "content": "Cyclical hypothalamic function was investigated in three patients with an XY karyotype and female external genitalia; in one of them we diagnosed gonadal agenesis, and in the other two testicular feminization. We studied the effect of estradiol and progesterone on gonadotropin release. The patient with gonadal agenesis had cyclical hypothalamic function, but this cyclical function was suppressed in the patients with testicular feminization in whom no LH secretion could be provoked by steroid stimulation. These observations support the concept that hypothalamic sexual differentiation is due to testosterone (which is locally converted to estradiol in the hypothalamus).", "contents": "Sexual differentiation of the hypothalamus in gonadal agenesis and testicular feminization. Cyclical hypothalamic function was investigated in three patients with an XY karyotype and female external genitalia; in one of them we diagnosed gonadal agenesis, and in the other two testicular feminization. We studied the effect of estradiol and progesterone on gonadotropin release. The patient with gonadal agenesis had cyclical hypothalamic function, but this cyclical function was suppressed in the patients with testicular feminization in whom no LH secretion could be provoked by steroid stimulation. These observations support the concept that hypothalamic sexual differentiation is due to testosterone (which is locally converted to estradiol in the hypothalamus)."}
{"id": "PMID:485566", "title": "[Ultrastructure of the secretory cells of the bovine prostatic gland: a possible secretion mechanism].", "content": "An ultrastructural study of the secretory cells of the Bovine Diseminated Prostatic Gland has been carried out. The main features of the glandular epithelium are described. The suggested mechanism of secretion is compared with those studied in other mammals. The proposed mechanism involves the formation of primary and secondary protrusions and accompanying isolating membrane systems. This process can be visualized in three different ultrastructural phases.", "contents": "[Ultrastructure of the secretory cells of the bovine prostatic gland: a possible secretion mechanism]. An ultrastructural study of the secretory cells of the Bovine Diseminated Prostatic Gland has been carried out. The main features of the glandular epithelium are described. The suggested mechanism of secretion is compared with those studied in other mammals. The proposed mechanism involves the formation of primary and secondary protrusions and accompanying isolating membrane systems. This process can be visualized in three different ultrastructural phases."}
{"id": "PMID:485567", "title": "[Seminal infection and male sterility].", "content": "A study is made of 25 patients suffering from a vesicular prostate pathology causing sterility. We have assessed the age, profession, symptoms, genital exploration and ejaculation study including the amount, pH, visocity, fructose, ascorbic acid, count, motility and morphology of the sperms and the high leukocyte reading. A seminal liquid culture has been carried out on 21 patients, alone or in association with cultures of prostate and urine secretion following prostate massaging with positive results in 16 of them (76%). Treatment was begun on 22 patients, in 13 of whom the results are known. Spermiogram readings became normal in 5 (38%) with one pregnancy; there was an associated pathology in the wife in 3 cases. As antibacterians we have basically used trimetoprim-sulphametoxazol associated with erythromycin or not and sometimes, according to the antibogram, with other antibiotics, as well as drugs to clear the prostate area. The best results were obtained in astenozoospermias. The effectiveness of the treatment was equally due to the use of TMP-SMZ on its own or along with erythromycin. The other drugs have not improved the results, except for minocyclin which megativated a sperm culture. As a preliminary result the author presented three patients with sperm infection produced by E. coli at whom the titer of antibodies in serum was investigated.", "contents": "[Seminal infection and male sterility]. A study is made of 25 patients suffering from a vesicular prostate pathology causing sterility. We have assessed the age, profession, symptoms, genital exploration and ejaculation study including the amount, pH, visocity, fructose, ascorbic acid, count, motility and morphology of the sperms and the high leukocyte reading. A seminal liquid culture has been carried out on 21 patients, alone or in association with cultures of prostate and urine secretion following prostate massaging with positive results in 16 of them (76%). Treatment was begun on 22 patients, in 13 of whom the results are known. Spermiogram readings became normal in 5 (38%) with one pregnancy; there was an associated pathology in the wife in 3 cases. As antibacterians we have basically used trimetoprim-sulphametoxazol associated with erythromycin or not and sometimes, according to the antibogram, with other antibiotics, as well as drugs to clear the prostate area. The best results were obtained in astenozoospermias. The effectiveness of the treatment was equally due to the use of TMP-SMZ on its own or along with erythromycin. The other drugs have not improved the results, except for minocyclin which megativated a sperm culture. As a preliminary result the author presented three patients with sperm infection produced by E. coli at whom the titer of antibodies in serum was investigated."}
{"id": "PMID:485568", "title": "[Traumatic sectioning of the isthmus in a case of renal fusion].", "content": "In this paper, we present a case of traumatic sectioning of the isthmus in a patient with multiple injuries, suffering from a horse-shoe-shaped kidney, which is extremely rare, according to the review of the literature which we have carried out. In view of the scanty incidence of kidney fusions and the difficulty sometimes involved in classifying them and in their surgical treatment, we shortly comment on the consequences which traumatisms may have on this kind of kidney malformation.", "contents": "[Traumatic sectioning of the isthmus in a case of renal fusion]. In this paper, we present a case of traumatic sectioning of the isthmus in a patient with multiple injuries, suffering from a horse-shoe-shaped kidney, which is extremely rare, according to the review of the literature which we have carried out. In view of the scanty incidence of kidney fusions and the difficulty sometimes involved in classifying them and in their surgical treatment, we shortly comment on the consequences which traumatisms may have on this kind of kidney malformation."}
{"id": "PMID:485569", "title": "[Postraumatic thrombosis of the renal artery].", "content": "We present two cases of thromboses of the left renal artery out of a total of 74 kidney traumatisms observed up to 1978. In one of them, accompanied by arterial hypertension, the tension readings returned to normal after a nephrectomy. Following a review of the literature, in which we observe the infrequency of this pathology (38 cases compiled by R. Fay and S. Brosman up to 1974), we comment upon the etiopathogeny, clinical characteristics, specific radiological signs and other means of exploration which enable the diagnosis to be reached. We point out the importance of an early diagnosis and the conservative and radical forms of surgery.", "contents": "[Postraumatic thrombosis of the renal artery]. We present two cases of thromboses of the left renal artery out of a total of 74 kidney traumatisms observed up to 1978. In one of them, accompanied by arterial hypertension, the tension readings returned to normal after a nephrectomy. Following a review of the literature, in which we observe the infrequency of this pathology (38 cases compiled by R. Fay and S. Brosman up to 1974), we comment upon the etiopathogeny, clinical characteristics, specific radiological signs and other means of exploration which enable the diagnosis to be reached. We point out the importance of an early diagnosis and the conservative and radical forms of surgery."}
{"id": "PMID:485571", "title": "[Urodynamics in female stress urinary incontinence].", "content": "Urodynamic studies are essential in order to rule out the existence of vesical instabilities. In our opinion, the urethral outlines are not always revealing and demand, apart from a highly planned technique, a critical and prudent assessment of the results.", "contents": "[Urodynamics in female stress urinary incontinence]. Urodynamic studies are essential in order to rule out the existence of vesical instabilities. In our opinion, the urethral outlines are not always revealing and demand, apart from a highly planned technique, a critical and prudent assessment of the results."}
{"id": "PMID:485570", "title": "[Evaluation of ultrasonics in extensive kidney processes. Study of 200 cases].", "content": "The author carries out a sonographic study on 200 expansive kidney processes, in which he assess the possibilities of this method of diagnosis at the same time offering the echographic semiology of the different disorders being studied. Finally, he explains the limitations and drawbacks of this technique.", "contents": "[Evaluation of ultrasonics in extensive kidney processes. Study of 200 cases]. The author carries out a sonographic study on 200 expansive kidney processes, in which he assess the possibilities of this method of diagnosis at the same time offering the echographic semiology of the different disorders being studied. Finally, he explains the limitations and drawbacks of this technique."}
{"id": "PMID:485575", "title": "Directed migration of circulating polymorphonuclear leucocytes in patients with rheumatoid arthritis: a defect in the plasma.", "content": "The migration of peripheral polymorphonuclear leucocytes (PMNs) of patients with rheumatoid arthritis has been studied both in vivo and in vitro. A significant reduction in the accumulation of PMNs in skin chambers in patients with rheumatoid arthritis compared to controls was observed but no defect in cell movement was detected when the isolated PMNs from the patients were exposed to activated control plasma. However, when PMNs from the control group were tested against activated plasma from patients with rheumatoid arthritis there was a significant decrease in their chemotactic response. It is proposed that there is a humoral defect in the plasma of the patients.", "contents": "Directed migration of circulating polymorphonuclear leucocytes in patients with rheumatoid arthritis: a defect in the plasma. The migration of peripheral polymorphonuclear leucocytes (PMNs) of patients with rheumatoid arthritis has been studied both in vivo and in vitro. A significant reduction in the accumulation of PMNs in skin chambers in patients with rheumatoid arthritis compared to controls was observed but no defect in cell movement was detected when the isolated PMNs from the patients were exposed to activated control plasma. However, when PMNs from the control group were tested against activated plasma from patients with rheumatoid arthritis there was a significant decrease in their chemotactic response. It is proposed that there is a humoral defect in the plasma of the patients."}
{"id": "PMID:485572", "title": "[Sisomicin sulfate: its efficacy in the treatment of urologic infections].", "content": "The authors explain the good results (80%) obtained with a new antibiotic belonging to the aminoglucoside group, the Sisomycin sulphate. Due to its active kidney excretion, its beneficial bactericide effect on Gram-positive and negative germs and its excellent tolerance, the author advises its use in infectious urological pathologies.", "contents": "[Sisomicin sulfate: its efficacy in the treatment of urologic infections]. The authors explain the good results (80%) obtained with a new antibiotic belonging to the aminoglucoside group, the Sisomycin sulphate. Due to its active kidney excretion, its beneficial bactericide effect on Gram-positive and negative germs and its excellent tolerance, the author advises its use in infectious urological pathologies."}
{"id": "PMID:485576", "title": "Abnormal polymorphonuclear leucocyte chemotaxis in Beh\u00e7et's syndrome.", "content": "Experiments both in vitro and in vivo have been performed to study the chemotactic response of polymorphonuclear leucocytes (PMNs) in Beh\u00e7et's syndrome. The experimental results were apparently contradictory. Using modified Boyden chambers we found that the PMNs from patients with Beh\u00e7et's syndrome responded to a greater extent in vitro than normal cells, but with skin chambers placed over abrasions the in vivo response was less than normal. The significance of these findings is discussed and related to the histological appearances that may be seen in this condition.", "contents": "Abnormal polymorphonuclear leucocyte chemotaxis in Beh\u00e7et's syndrome. Experiments both in vitro and in vivo have been performed to study the chemotactic response of polymorphonuclear leucocytes (PMNs) in Beh\u00e7et's syndrome. The experimental results were apparently contradictory. Using modified Boyden chambers we found that the PMNs from patients with Beh\u00e7et's syndrome responded to a greater extent in vitro than normal cells, but with skin chambers placed over abrasions the in vivo response was less than normal. The significance of these findings is discussed and related to the histological appearances that may be seen in this condition."}
{"id": "PMID:485577", "title": "Airways obstruction in rheumatoid arthritis.", "content": "Owing to the report of an association between rheumatoid arthritis (RA) and obliterative bronchiolitis we have determined the prevalence of airflow obstruction in unselected patients with RA and normal chest radiographs. Spirometry was performed on 100 patients with rheumatoid arthritis and 84 control subjects matched for age, sex, and smoking habits. Patients with rheumatoid arthritis had significantly lower values for FEV1, FVC, FEV1/FVC, and MMEFR when compared with the controls: 39 patients had abnormal spirometry, and at least 32 showed airways obstruction. The prevalence of airflow obstruction is remarkably high, and we suggest that airway disease may be the commonest form of lung involvement in rheumatoid arthritis.", "contents": "Airways obstruction in rheumatoid arthritis. Owing to the report of an association between rheumatoid arthritis (RA) and obliterative bronchiolitis we have determined the prevalence of airflow obstruction in unselected patients with RA and normal chest radiographs. Spirometry was performed on 100 patients with rheumatoid arthritis and 84 control subjects matched for age, sex, and smoking habits. Patients with rheumatoid arthritis had significantly lower values for FEV1, FVC, FEV1/FVC, and MMEFR when compared with the controls: 39 patients had abnormal spirometry, and at least 32 showed airways obstruction. The prevalence of airflow obstruction is remarkably high, and we suggest that airway disease may be the commonest form of lung involvement in rheumatoid arthritis."}
{"id": "PMID:485578", "title": "The nail dystrophy of psoriatic arthritis.", "content": "Nail abnormalities occur frequently in patients with psoriatic arthritis. This study of the finger nails of 46 patients with psoriatic arthritis, 100 nonpsoriatic rheumatism patients, and 100 nonpsoriatic general medical patients was designed to characterise these abnormalities with particular reference to the severity of nail pitting. The results of the study suggest: (1) Onycholysis alone in the absence of previous injury to the affected nail is in favour of a psoriatic origin for the nail dystrophy. (2) Two or all of onycholysis, horizontal ridging, and nail pitting in the same patient are in favour of a psoriatic origin for the nail dystrophy. (3) The presence or absence of nail pitting alone is a poor discriminator between psoriatic and other causes for nail dystrophy. (4) More than 20 finger nails pits per person is suggestive of a psoriatic cause for the dystrophy. (5) More than 60 pits per person is unlikely to be found in the absence of psoriasis.", "contents": "The nail dystrophy of psoriatic arthritis. Nail abnormalities occur frequently in patients with psoriatic arthritis. This study of the finger nails of 46 patients with psoriatic arthritis, 100 nonpsoriatic rheumatism patients, and 100 nonpsoriatic general medical patients was designed to characterise these abnormalities with particular reference to the severity of nail pitting. The results of the study suggest: (1) Onycholysis alone in the absence of previous injury to the affected nail is in favour of a psoriatic origin for the nail dystrophy. (2) Two or all of onycholysis, horizontal ridging, and nail pitting in the same patient are in favour of a psoriatic origin for the nail dystrophy. (3) The presence or absence of nail pitting alone is a poor discriminator between psoriatic and other causes for nail dystrophy. (4) More than 20 finger nails pits per person is suggestive of a psoriatic cause for the dystrophy. (5) More than 60 pits per person is unlikely to be found in the absence of psoriasis."}
{"id": "PMID:485573", "title": "[Urine cultures and antibiograms].", "content": "A review is made of the urocultures and antibiograms carried out in a period of two months in the Urology Department of a General Hospital. A comparative study is made with the other bacteriological results sent to the laboratory from the different Departments. A study is made of the sensitivity of the isolated stocks to the most widely used antibiotics.", "contents": "[Urine cultures and antibiograms]. A review is made of the urocultures and antibiograms carried out in a period of two months in the Urology Department of a General Hospital. A comparative study is made with the other bacteriological results sent to the laboratory from the different Departments. A study is made of the sensitivity of the isolated stocks to the most widely used antibiotics."}
{"id": "PMID:485579", "title": "Resumption of treatment with penicillamine after proteinuria.", "content": "Penicillamine has been successfully reintroduced and continued for a minimum of 13 months in 5 patients who developed proteinuria during the first course of the drug. The daily maintenance dose during the second course was 150--250 mg taken midway between 2 meals. Proteinuria did not recur; no significant excretion of fibrin degradation products occurred; complement, urea, creatinine, and serum albumin remained within normal limits. Urine microscopy showed no abnormality.", "contents": "Resumption of treatment with penicillamine after proteinuria. Penicillamine has been successfully reintroduced and continued for a minimum of 13 months in 5 patients who developed proteinuria during the first course of the drug. The daily maintenance dose during the second course was 150--250 mg taken midway between 2 meals. Proteinuria did not recur; no significant excretion of fibrin degradation products occurred; complement, urea, creatinine, and serum albumin remained within normal limits. Urine microscopy showed no abnormality."}
{"id": "PMID:485580", "title": "Myelotoxicity of D-penicillamine.", "content": "Information has been collected on 10 patients, 9 with marrow depression and 1 in whom the diagnosis was presumed. Six of the 10 patients died. The sequentially recorded blood counts on at least 5 of the patients showed a downward trend of the white cell and platelet counts while D-penicillamine was still being administered. One patient suddenly developed leucopenia and thrombocytopenia with a streptococcal septicaemia.", "contents": "Myelotoxicity of D-penicillamine. Information has been collected on 10 patients, 9 with marrow depression and 1 in whom the diagnosis was presumed. Six of the 10 patients died. The sequentially recorded blood counts on at least 5 of the patients showed a downward trend of the white cell and platelet counts while D-penicillamine was still being administered. One patient suddenly developed leucopenia and thrombocytopenia with a streptococcal septicaemia."}
{"id": "PMID:485581", "title": "Quantitative sacroiliac scintigraphy in ankylosing spondylitis and Crohn's disease: a single family study.", "content": "A single family is presented in which 2 of the 3 sibs and 3 of 9 first degree relatives had Crohn's disease. Two subjects with Crohn's disease had classical ankylosing spondylitis (AS), and HLA typing revealed HLA B27 in all 5 members of the immediate family. The value of quantitative sacroiliac scintigraphy (QSS) in clinical practice is demonstrated. Both patients with AS had evidence of currently active sacroiliac disease in association with radiological change. One symptomatic sib had QSS evidence of sacroiliitis without radiological abnormality.", "contents": "Quantitative sacroiliac scintigraphy in ankylosing spondylitis and Crohn's disease: a single family study. A single family is presented in which 2 of the 3 sibs and 3 of 9 first degree relatives had Crohn's disease. Two subjects with Crohn's disease had classical ankylosing spondylitis (AS), and HLA typing revealed HLA B27 in all 5 members of the immediate family. The value of quantitative sacroiliac scintigraphy (QSS) in clinical practice is demonstrated. Both patients with AS had evidence of currently active sacroiliac disease in association with radiological change. One symptomatic sib had QSS evidence of sacroiliitis without radiological abnormality."}
{"id": "PMID:485582", "title": "Thrombokinetics in giant cell arteritis, with special reference to corticosteroid therapy.", "content": "Duplicate platelet survival studies were carried out on 8 patients with giant cell arteritis (GCA), once before the institution of any therapy, and the second time when they were in a completely asymptomatic phase after having received corticosteroid treatment. The time interval between the studies ranged between 5 and 14 months. In the first study the mean peripheral platelet count was 486 +/- 25 X 10(9)/l and in the second 326 +/- 25 X 10(9)/l. The difference between the means was highly significant (P less than 0.001). The mean life-span of the platelets was normal in the duplicate experiments (6.7 +/- 0.3 and 7.3 +/- 0.4 days, respectively). Platelet production rate was significantly (P less than 0.001) raised in the first experiment but became normal in response to corticosteroid therapy. It is concluded that the thrombocytosis seen in GCA is reactive to the inflammation present in this disease, and it seems reasonable to assume that the reduction in the peripheral platelet count which occurs in response to corticosteroid therapy accurately reflects the clinical improvement of the patient.", "contents": "Thrombokinetics in giant cell arteritis, with special reference to corticosteroid therapy. Duplicate platelet survival studies were carried out on 8 patients with giant cell arteritis (GCA), once before the institution of any therapy, and the second time when they were in a completely asymptomatic phase after having received corticosteroid treatment. The time interval between the studies ranged between 5 and 14 months. In the first study the mean peripheral platelet count was 486 +/- 25 X 10(9)/l and in the second 326 +/- 25 X 10(9)/l. The difference between the means was highly significant (P less than 0.001). The mean life-span of the platelets was normal in the duplicate experiments (6.7 +/- 0.3 and 7.3 +/- 0.4 days, respectively). Platelet production rate was significantly (P less than 0.001) raised in the first experiment but became normal in response to corticosteroid therapy. It is concluded that the thrombocytosis seen in GCA is reactive to the inflammation present in this disease, and it seems reasonable to assume that the reduction in the peripheral platelet count which occurs in response to corticosteroid therapy accurately reflects the clinical improvement of the patient."}
{"id": "PMID:485583", "title": "Behaviour of effector cells, synovial fluids, and sera from rheumatoid arthritis patients in antibody-dependent cell-mediated cytotoxicity.", "content": "Antibody-dependent cell-mediated cytotoxicity (ADCC) was examined in patients with rheumatoid arthritis (RA). The cytotoxicity of peripheral blood leucocytes from patients with RA was similar to that found in normal persons, whereas ADCC was less effective in RA synovial fluid cells. It is possible that the activity in these cells is lower because of immune complexes and other factors being absorbed from the synovial fluid itself. Although patients' sera had little effect on normal peripheral blood leucocytes, synovial fluid from RA patients was markedly inhibitory in ADCC. The degree of inhibition correlated significantly with the clinical status of the patients.", "contents": "Behaviour of effector cells, synovial fluids, and sera from rheumatoid arthritis patients in antibody-dependent cell-mediated cytotoxicity. Antibody-dependent cell-mediated cytotoxicity (ADCC) was examined in patients with rheumatoid arthritis (RA). The cytotoxicity of peripheral blood leucocytes from patients with RA was similar to that found in normal persons, whereas ADCC was less effective in RA synovial fluid cells. It is possible that the activity in these cells is lower because of immune complexes and other factors being absorbed from the synovial fluid itself. Although patients' sera had little effect on normal peripheral blood leucocytes, synovial fluid from RA patients was markedly inhibitory in ADCC. The degree of inhibition correlated significantly with the clinical status of the patients."}
{"id": "PMID:485584", "title": "Effect of hyperoxia on articular tissues in organ culture.", "content": "The effect of hyperoxia on pig articular tissue has been studied in organ culture. Hyperoxia (55% O2) causes an increased release of hydroxyproline and collagenolytic activity from synovial tissue as compared with control explants in 20% O2, but neither 55% nor 95% O2 has any effect on the breakdown of isolated cartilage during 10 days in culture. When synovium and cartilage are cultured in contact, the breakdown of cartilage collagen is greater in the hyperoxic (55% O2) group than in the controls (20% O2), but the breakdown of proteoglycan is not increased. The enhanced collagenolytic action is due to an increase in the direct effect of the synovial tissue on the cartilage matrix. In 20% O2 the synovium causes the chondrocytes to degrade the cartilage matrix, but this effect is inhibited by 55% O2.", "contents": "Effect of hyperoxia on articular tissues in organ culture. The effect of hyperoxia on pig articular tissue has been studied in organ culture. Hyperoxia (55% O2) causes an increased release of hydroxyproline and collagenolytic activity from synovial tissue as compared with control explants in 20% O2, but neither 55% nor 95% O2 has any effect on the breakdown of isolated cartilage during 10 days in culture. When synovium and cartilage are cultured in contact, the breakdown of cartilage collagen is greater in the hyperoxic (55% O2) group than in the controls (20% O2), but the breakdown of proteoglycan is not increased. The enhanced collagenolytic action is due to an increase in the direct effect of the synovial tissue on the cartilage matrix. In 20% O2 the synovium causes the chondrocytes to degrade the cartilage matrix, but this effect is inhibited by 55% O2."}
{"id": "PMID:485586", "title": "[Seroepidemiological considerations on the influenza a in Umbria (1978) (author's transl)].", "content": "The authors studied the seroepidemiology of influenza virus A/Victoria/3/75 (H3N2) and A/URSS/90/77 (H1N1) in Umbria, in 1978. The percentage of seroprotection against the subtype H3N2 results low and in such do not inhibit the diffusion of this subtype during the next winter season. The study of seroepidemiology of subtype H1N1 demonstrated that this subtype circulated in Umbria in the winter 1977-78 and that the conditions exist for its circulation.", "contents": "[Seroepidemiological considerations on the influenza a in Umbria (1978) (author's transl)]. The authors studied the seroepidemiology of influenza virus A/Victoria/3/75 (H3N2) and A/URSS/90/77 (H1N1) in Umbria, in 1978. The percentage of seroprotection against the subtype H3N2 results low and in such do not inhibit the diffusion of this subtype during the next winter season. The study of seroepidemiology of subtype H1N1 demonstrated that this subtype circulated in Umbria in the winter 1977-78 and that the conditions exist for its circulation."}
{"id": "PMID:485587", "title": "[Rubella epidemic of 1973 in Umbria: seroepidemiologic study (author's transl)].", "content": "The rubella seroepidemiology before 1973 and the epidemic outbreak of rubella in 1973, in Umbria was studied. Antibody movement by specific activity of IgG and IgM was shown, and characterized by duration and in the various phases of the disease and post-disease.", "contents": "[Rubella epidemic of 1973 in Umbria: seroepidemiologic study (author's transl)]. The rubella seroepidemiology before 1973 and the epidemic outbreak of rubella in 1973, in Umbria was studied. Antibody movement by specific activity of IgG and IgM was shown, and characterized by duration and in the various phases of the disease and post-disease."}
{"id": "PMID:485588", "title": "[Study on the immunoglobulin class of the antibody anti-e in acute HBsAg+ hepatitis and in asymptomatic carriers of HBsAg (author's transl)].", "content": "The AA. have determined the immunoglobulinic appartenence class of HBeAb in patients with acute hepatitis HBsAg+ and in asymptomatic carriers of HBsAg. In these the HBeAb belongs constantly to the IgG class, while in acute hepatitis patients this antibody results present in the IgM and IgG fractions of the sera. Especially, in patients with acute hepatitis the presence of HBeAb was observed only in some sera obtained during the tardive phase of disease.", "contents": "[Study on the immunoglobulin class of the antibody anti-e in acute HBsAg+ hepatitis and in asymptomatic carriers of HBsAg (author's transl)]. The AA. have determined the immunoglobulinic appartenence class of HBeAb in patients with acute hepatitis HBsAg+ and in asymptomatic carriers of HBsAg. In these the HBeAb belongs constantly to the IgG class, while in acute hepatitis patients this antibody results present in the IgM and IgG fractions of the sera. Especially, in patients with acute hepatitis the presence of HBeAb was observed only in some sera obtained during the tardive phase of disease."}
{"id": "PMID:485589", "title": "[An epidemic of familial toxoplasmosis (author's transl)].", "content": "After observation of choroiditis of toxoplasmic nature in a member of a rural family, the authors have led a clinical and serological study on other five members of the household. Three of five had antibody titers and two of these demonstrated antibody titers consistent with recent toxoplasmic infection; one of these members manifested asymptomatic choroiditis. Therefore, it would be interesting to carry out a clinical and serological study on relatives of patient with toxoplasmosis.", "contents": "[An epidemic of familial toxoplasmosis (author's transl)]. After observation of choroiditis of toxoplasmic nature in a member of a rural family, the authors have led a clinical and serological study on other five members of the household. Three of five had antibody titers and two of these demonstrated antibody titers consistent with recent toxoplasmic infection; one of these members manifested asymptomatic choroiditis. Therefore, it would be interesting to carry out a clinical and serological study on relatives of patient with toxoplasmosis."}
{"id": "PMID:485590", "title": "[Widal-Wright: a semiautomatic micromethod (author's transl)].", "content": "A semiautomatic micromethod is explained to execute the agglutination reaction of Widal type. It is reported the perfect agreement existing between the above-mentioned method and the traditional one, in the test tube. The semiautomatic method is particularly fit for the standardisation of the Widal reaction and moreover it avoids some disadvantages by this method presented.", "contents": "[Widal-Wright: a semiautomatic micromethod (author's transl)]. A semiautomatic micromethod is explained to execute the agglutination reaction of Widal type. It is reported the perfect agreement existing between the above-mentioned method and the traditional one, in the test tube. The semiautomatic method is particularly fit for the standardisation of the Widal reaction and moreover it avoids some disadvantages by this method presented."}
{"id": "PMID:485591", "title": "[Salmonellosis in Messina during 1975-1977: epidemiological considerations and prophylactic recommendations (author's transl)].", "content": "The AA. report the results of the bacteriological researches carried during the years 1975-1977 on 8226 cultures. They remark the high percentage of positivity registered for Salmonella, with superiority of S. wien (87.74%), and the onset of serotypes of group E1. At the conclusion of their study point out the nature endemic taken by the salmonellosis in Messina, and suggest the practice of prophylaxis for the control of the epidemiology.", "contents": "[Salmonellosis in Messina during 1975-1977: epidemiological considerations and prophylactic recommendations (author's transl)]. The AA. report the results of the bacteriological researches carried during the years 1975-1977 on 8226 cultures. They remark the high percentage of positivity registered for Salmonella, with superiority of S. wien (87.74%), and the onset of serotypes of group E1. At the conclusion of their study point out the nature endemic taken by the salmonellosis in Messina, and suggest the practice of prophylaxis for the control of the epidemiology."}
{"id": "PMID:485592", "title": "[Prevalence and transferability of the plasmid lac+ character in \"Klebsiella\" sp. strains with \"lac+-forte\" phenotype].", "content": "36 strains of Klebsiella sp. showing a strong lactose-positive phenotype were examined for presence of R factors and for ability to promote the transfer of character lac+; 10 of the 13 R+ strains were able to transfer lac+ character and 4 showed the presence of R-lac factors. Transfer of R-lac factors from Klebsiella sp. strains to Salmonella typhi clinical isolates has also been obtained.", "contents": "[Prevalence and transferability of the plasmid lac+ character in \"Klebsiella\" sp. strains with \"lac+-forte\" phenotype]. 36 strains of Klebsiella sp. showing a strong lactose-positive phenotype were examined for presence of R factors and for ability to promote the transfer of character lac+; 10 of the 13 R+ strains were able to transfer lac+ character and 4 showed the presence of R-lac factors. Transfer of R-lac factors from Klebsiella sp. strains to Salmonella typhi clinical isolates has also been obtained."}
{"id": "PMID:485593", "title": "[Incidence of antibodies inhibiting hemagglutination by influenza virus A/New Jersey/8/76 (Hws1N1) in sera of swine and the population of Umbria].", "content": "The authors studied the seroepidemiology of influenza virus A/New Jersey/8/76 (Hsw1N1) in Umbria, in animals and human populations. Human samples demonstrated seropositive evidence only in those subjects with an age above 50 years. The results from swine samples instead proves the circulation of virus in pigs.", "contents": "[Incidence of antibodies inhibiting hemagglutination by influenza virus A/New Jersey/8/76 (Hws1N1) in sera of swine and the population of Umbria]. The authors studied the seroepidemiology of influenza virus A/New Jersey/8/76 (Hsw1N1) in Umbria, in animals and human populations. Human samples demonstrated seropositive evidence only in those subjects with an age above 50 years. The results from swine samples instead proves the circulation of virus in pigs."}
{"id": "PMID:485594", "title": "[Identification and antibiotic-resistance of microbial strains isolated from urinary infections (author's transl)].", "content": "During the year 1977, 1491 urine samples were taken from patients living in the \"Alta Valle del Tevere\" (Umbria, Italy). The isolated microorganisms were examined and tested against 15 antibiotics. 535 samples (35.88%) were free from bacteria. Among the 1095 isolates, 52.42% were Gram-negative, 46.85% Gram-positive, and only 0.73% Fungi. The genera Citrobacter, Escherichia, Proteus, Pseudomonas, Enterobacter, Klebsiella, Staphylococcus and Enterococcus were identified. A high proportion of strains belonging to the genera Proteus and Pseudomonas were resistant to all the 15 antibiotics tested. However, although in a lower proportion, the multiple drug-resistance was present also in the strains of the genera Escherichia and Staphylococcus.", "contents": "[Identification and antibiotic-resistance of microbial strains isolated from urinary infections (author's transl)]. During the year 1977, 1491 urine samples were taken from patients living in the \"Alta Valle del Tevere\" (Umbria, Italy). The isolated microorganisms were examined and tested against 15 antibiotics. 535 samples (35.88%) were free from bacteria. Among the 1095 isolates, 52.42% were Gram-negative, 46.85% Gram-positive, and only 0.73% Fungi. The genera Citrobacter, Escherichia, Proteus, Pseudomonas, Enterobacter, Klebsiella, Staphylococcus and Enterococcus were identified. A high proportion of strains belonging to the genera Proteus and Pseudomonas were resistant to all the 15 antibiotics tested. However, although in a lower proportion, the multiple drug-resistance was present also in the strains of the genera Escherichia and Staphylococcus."}
{"id": "PMID:485595", "title": "[New considerations on the role of the mouse skin surface lipids on the \"in vivo\" penetration of \"Schistosoma mansoni\" cercariae (author's transl)].", "content": "The authors have studied the effect of delipitizing and of the previously extracted skin lipids reapplication on mice experimental infected with Schistosoma mansoni cercariae. The results demonstrated that the amount of living cercariae, not penetrated, recovered after the exposition to the parasite is greater in the delipitized mice than in the other ones. The low amount of worms recovered from the treated mice might be due to the effect of the mouse skin surface lipids not only on stimulating cercarial penetration but also on allowing cercarial transformation to schistosomula during skin crossing.", "contents": "[New considerations on the role of the mouse skin surface lipids on the \"in vivo\" penetration of \"Schistosoma mansoni\" cercariae (author's transl)]. The authors have studied the effect of delipitizing and of the previously extracted skin lipids reapplication on mice experimental infected with Schistosoma mansoni cercariae. The results demonstrated that the amount of living cercariae, not penetrated, recovered after the exposition to the parasite is greater in the delipitized mice than in the other ones. The low amount of worms recovered from the treated mice might be due to the effect of the mouse skin surface lipids not only on stimulating cercarial penetration but also on allowing cercarial transformation to schistosomula during skin crossing."}
{"id": "PMID:485596", "title": "[CIEP with cellogel (cellulose acetate membrane) in the diagnosis of visceral leishmaniosis (author's transl)].", "content": "Technique of counter immunoelectrophoresis (CIEP) was employed for the diagnosis of V.L. (human and canine) using strips of cellulose acetate and an antigen grossly extracted (by means of repeated freezing and thawing) from culture of Leishmania. 4 lots of antigen was prepared, in various time, at the same way. Positive results were obtained in V.L. from 81 to 90% (according to various lots of antigen). False positivities (from 1 to 4.5%) occur in patient with other diseases (especially cirrhosis and blood disorders). None positivity in controls (blood donors). Present results and those obtained with same technique in agar, suggested the validity of method and encouraged the production of antigen in a \"kit\" form for use in the field.", "contents": "[CIEP with cellogel (cellulose acetate membrane) in the diagnosis of visceral leishmaniosis (author's transl)]. Technique of counter immunoelectrophoresis (CIEP) was employed for the diagnosis of V.L. (human and canine) using strips of cellulose acetate and an antigen grossly extracted (by means of repeated freezing and thawing) from culture of Leishmania. 4 lots of antigen was prepared, in various time, at the same way. Positive results were obtained in V.L. from 81 to 90% (according to various lots of antigen). False positivities (from 1 to 4.5%) occur in patient with other diseases (especially cirrhosis and blood disorders). None positivity in controls (blood donors). Present results and those obtained with same technique in agar, suggested the validity of method and encouraged the production of antigen in a \"kit\" form for use in the field."}
{"id": "PMID:485597", "title": "[New methods on the measles prophylaxis (author's transl)].", "content": "As for the preventive measures used towards patients with a measles infection, the Authors review in particular the new methods of isolation on the basis of the latest acquisitions on the way and period of contagiousness. As for the preventive measures for healthy people, the Authors review in particular the vaccination route. After having considered the principal types of vaccine used, they demonstrate the efficiency and harmlesness of the live and attenuated virus vaccine, in general, and in particular the \"hyperattenuated\" vaccine, reviewing the principal causes of the failure of such a vaccine in some cases. They give an ample documentation of the period of immunity (persistance of HAI antibodies) in the vaccined. Considering the experience of other countries, the Authors suggest that it is time to procede in a large scale vaccination in Italy, underlining the fundamental points (use of live and hyperattenuated vaccine, appropriate storage and care of the vaccine, age of vaccination) to prevent failure and to make more and more efficient this type of vaccination.", "contents": "[New methods on the measles prophylaxis (author's transl)]. As for the preventive measures used towards patients with a measles infection, the Authors review in particular the new methods of isolation on the basis of the latest acquisitions on the way and period of contagiousness. As for the preventive measures for healthy people, the Authors review in particular the vaccination route. After having considered the principal types of vaccine used, they demonstrate the efficiency and harmlesness of the live and attenuated virus vaccine, in general, and in particular the \"hyperattenuated\" vaccine, reviewing the principal causes of the failure of such a vaccine in some cases. They give an ample documentation of the period of immunity (persistance of HAI antibodies) in the vaccined. Considering the experience of other countries, the Authors suggest that it is time to procede in a large scale vaccination in Italy, underlining the fundamental points (use of live and hyperattenuated vaccine, appropriate storage and care of the vaccine, age of vaccination) to prevent failure and to make more and more efficient this type of vaccination."}
{"id": "PMID:485598", "title": "[Mathematical models in epidemiology: Study on viral hepatitis in Italy (author's transl)].", "content": "In this paper a mathematical approach to the epidemics is proposed. The method is based upon a model able to describe any time-depending phenomena using the following elements: \"class\", \"transition\" and \"related probability\". The solution of the differential equations describing the model are obtained: first, by numerical techniques; second, by a Montecarlo simulation method. Deterministic and stochastic solutions which have been obtained, by applying the model to the study of viral hepatitis in Italy during 1960--1970, have been compared each other, to improve the model itself.", "contents": "[Mathematical models in epidemiology: Study on viral hepatitis in Italy (author's transl)]. In this paper a mathematical approach to the epidemics is proposed. The method is based upon a model able to describe any time-depending phenomena using the following elements: \"class\", \"transition\" and \"related probability\". The solution of the differential equations describing the model are obtained: first, by numerical techniques; second, by a Montecarlo simulation method. Deterministic and stochastic solutions which have been obtained, by applying the model to the study of viral hepatitis in Italy during 1960--1970, have been compared each other, to improve the model itself."}
{"id": "PMID:485602", "title": "Glucose requirements following burn injury. Parameters of optimal glucose infusion and possible hepatic and respiratory abnormalities following excessive glucose intake.", "content": "Glucose and leucine metabolism in 18 severely burned patients were studied using the primed constant infusion of U-13C-glucose and 1-13C-leucine, respectively. The leucine data were used to calculate rates of whole-body protein synthesis. In four additional burn patients and seven normal controls, the effects of exogenously infused insulin on the metabolism of infused glucose were evaluated. Also, the effect on leucine metabolism of adding insulin to infused glucose was tested and rates of protein synthesis were calculated. The protein studies were divided into two groups depending on the rate of glucose infusion. Protein synthesis was 4.3 + 0.54 g protein/kg/day during the lower infusion rates (1.4--4.5 mg/kg/min) and 5.17 + 0.19 g protein/kg/day during the higher infusion rates (4.7--9.3 mg/kg/min) (statistically different, p less than 0.05). However, when the high infusion rate group was divided into two subgroups (high, 4.7--6.8 mg/kg/min, and very high, 7.03--9.31 mg/kg/min), there was no difference in the rate of protein synthesis. When U-13C-glucose was infused during varying rates of unlabeled glucose infusion, we found that the per cent of CO2 coming from the direct oxidation of glucose rose rapidly at the lower infusion rates but reached a plateau at approximately 55% as the infusion rates exceeded 5 mg/kg/min. Addition of insulin did not affect the rate of glucose oxidation but did seem to exert a stimulatory effect on protein synthesis. It was concluded that there appears to be a maximal rate of glucose infusion, beyond which physiologically significant increases in protein synthesis and direct oxidation of glucose cannot be expected. Furthermore, there appears to be a physiological cost of exceeding the optimal glucose infusion rate, as indicated by increased rates of CO2 production during infusion as well as large fat deposits in the liver at autopsy in patients infused with large amounts of glucose.", "contents": "Glucose requirements following burn injury. Parameters of optimal glucose infusion and possible hepatic and respiratory abnormalities following excessive glucose intake. Glucose and leucine metabolism in 18 severely burned patients were studied using the primed constant infusion of U-13C-glucose and 1-13C-leucine, respectively. The leucine data were used to calculate rates of whole-body protein synthesis. In four additional burn patients and seven normal controls, the effects of exogenously infused insulin on the metabolism of infused glucose were evaluated. Also, the effect on leucine metabolism of adding insulin to infused glucose was tested and rates of protein synthesis were calculated. The protein studies were divided into two groups depending on the rate of glucose infusion. Protein synthesis was 4.3 + 0.54 g protein/kg/day during the lower infusion rates (1.4--4.5 mg/kg/min) and 5.17 + 0.19 g protein/kg/day during the higher infusion rates (4.7--9.3 mg/kg/min) (statistically different, p less than 0.05). However, when the high infusion rate group was divided into two subgroups (high, 4.7--6.8 mg/kg/min, and very high, 7.03--9.31 mg/kg/min), there was no difference in the rate of protein synthesis. When U-13C-glucose was infused during varying rates of unlabeled glucose infusion, we found that the per cent of CO2 coming from the direct oxidation of glucose rose rapidly at the lower infusion rates but reached a plateau at approximately 55% as the infusion rates exceeded 5 mg/kg/min. Addition of insulin did not affect the rate of glucose oxidation but did seem to exert a stimulatory effect on protein synthesis. It was concluded that there appears to be a maximal rate of glucose infusion, beyond which physiologically significant increases in protein synthesis and direct oxidation of glucose cannot be expected. Furthermore, there appears to be a physiological cost of exceeding the optimal glucose infusion rate, as indicated by increased rates of CO2 production during infusion as well as large fat deposits in the liver at autopsy in patients infused with large amounts of glucose."}
{"id": "PMID:485603", "title": "Correlation between anergy and a circulating immunosuppressive factor following major surgical trauma.", "content": "In order to clarify the relationship between anergy and immunosuppressive activity in the serum, we studied 46 previously well patients before and at three, five, seven and 28 days after surgery. Delayed hypersensitivity was measured by skin testing with four common recall antigens, and serum immunosuppressive activity was determined by the ability of the patient's serum in 10% concentration to suppress by 50% or more the phytohemagglutinin (PHA) stimulation of normal human lymphocytes as compared to pooled normal serum. Prior to surgery, all patients manifested delayed hypersensitivity to one or more antigens, and no patient had immunosuppressive serum. Fifteen patients underwent minor surgery under general anesthesia and did not develop anergy or immunosuppressive serum. Thirty-one patients underwent major cardiovascular surgery. Thirteen of these patients became anergic by day 3 after operation, and 11 of the 13 developed immunosuppressive serum. Eighteen patients maintained delayed hypersensitivity after major surgery, and only three developed immunosuppressive serum. The correlation between anergy and immunosuppressive serum was highly significant (p < 0.001). There was a significant difference in the degree of suppressive activity in the serum of the anergic and reactive patient groups for each postoperative day studied until day 28, when there was recovery of delayed hypersensitivity and lack of immunosuppressive serum. The occurrence of postoperative anergy and immunosuppressive serum was not related to the patient's age, sex, number of perioperative blood transfusions or duration of anesthesia but was associated with an increase in postoperative infectious complications (p < 0.05) and in postoperative days in the hospital (p < 0.01). Pooled immunosuppressive serum from anergic patients was fractionated by ion exchange chromatography, gel filtration and preparative high voltage electrophoresis. The majority of the immunosuppressive activity could be accounted for by an electrophoretically homogenous polypeptide-containing fraction not identified in the serum of patients undergoing minor surgery or in normal individuals. We conclude that anergy occurring after major operative trauma is associated with the appearance of a circulating immunosuppressive molecular species and that these events are in turn associated with increased patient morbidity and increased length of hospitalization.", "contents": "Correlation between anergy and a circulating immunosuppressive factor following major surgical trauma. In order to clarify the relationship between anergy and immunosuppressive activity in the serum, we studied 46 previously well patients before and at three, five, seven and 28 days after surgery. Delayed hypersensitivity was measured by skin testing with four common recall antigens, and serum immunosuppressive activity was determined by the ability of the patient's serum in 10% concentration to suppress by 50% or more the phytohemagglutinin (PHA) stimulation of normal human lymphocytes as compared to pooled normal serum. Prior to surgery, all patients manifested delayed hypersensitivity to one or more antigens, and no patient had immunosuppressive serum. Fifteen patients underwent minor surgery under general anesthesia and did not develop anergy or immunosuppressive serum. Thirty-one patients underwent major cardiovascular surgery. Thirteen of these patients became anergic by day 3 after operation, and 11 of the 13 developed immunosuppressive serum. Eighteen patients maintained delayed hypersensitivity after major surgery, and only three developed immunosuppressive serum. The correlation between anergy and immunosuppressive serum was highly significant (p < 0.001). There was a significant difference in the degree of suppressive activity in the serum of the anergic and reactive patient groups for each postoperative day studied until day 28, when there was recovery of delayed hypersensitivity and lack of immunosuppressive serum. The occurrence of postoperative anergy and immunosuppressive serum was not related to the patient's age, sex, number of perioperative blood transfusions or duration of anesthesia but was associated with an increase in postoperative infectious complications (p < 0.05) and in postoperative days in the hospital (p < 0.01). Pooled immunosuppressive serum from anergic patients was fractionated by ion exchange chromatography, gel filtration and preparative high voltage electrophoresis. The majority of the immunosuppressive activity could be accounted for by an electrophoretically homogenous polypeptide-containing fraction not identified in the serum of patients undergoing minor surgery or in normal individuals. We conclude that anergy occurring after major operative trauma is associated with the appearance of a circulating immunosuppressive molecular species and that these events are in turn associated with increased patient morbidity and increased length of hospitalization."}
{"id": "PMID:485599", "title": "[Bacteriological diagnosis of anaerobic infections (author's transl)].", "content": "A review on anaerobic bacteria with special reference to their role in human disease and an outline for clinical laboratory practice (collection of the specimens, culture media, isolation, identification, antibiotic sensitivity tests) is presented.", "contents": "[Bacteriological diagnosis of anaerobic infections (author's transl)]. A review on anaerobic bacteria with special reference to their role in human disease and an outline for clinical laboratory practice (collection of the specimens, culture media, isolation, identification, antibiotic sensitivity tests) is presented."}
{"id": "PMID:485600", "title": "[The evaluation of antibody-coated bacteria in the urine sediment to watch the efficiency of antibacterial treatment in upper urinary-tract infections (author's transl)].", "content": "The effect of up to 6months antibacterial treatment on both urine culture and the presence of antibody-coated bacteria in the urinary sediment was examined in 15 patients with upper urinary-tract infection. At the end of the sixth month all the patients had sterile urine, while about one half still demonstrated antibody-coated bacteria in their sediment. Such a finding strongly suggests that urine culture is \"per se\" insufficient to exclude the presence of bacteria in the renal parenchyma, and that the evaluation of antibody-coated bacteria might be a better way to watch the effect of treatment in upper urinary-tract infections.", "contents": "[The evaluation of antibody-coated bacteria in the urine sediment to watch the efficiency of antibacterial treatment in upper urinary-tract infections (author's transl)]. The effect of up to 6months antibacterial treatment on both urine culture and the presence of antibody-coated bacteria in the urinary sediment was examined in 15 patients with upper urinary-tract infection. At the end of the sixth month all the patients had sterile urine, while about one half still demonstrated antibody-coated bacteria in their sediment. Such a finding strongly suggests that urine culture is \"per se\" insufficient to exclude the presence of bacteria in the renal parenchyma, and that the evaluation of antibody-coated bacteria might be a better way to watch the effect of treatment in upper urinary-tract infections."}
{"id": "PMID:485604", "title": "Vasodilator effects of the sodium acetate in pooled protein fraction.", "content": "Paradoxical hypotension during rapid infusion of plasma protein fraction (PPF) has been attributed to vasodilation by bradykinin in PPF. This study employed a canine, controlled right heart bypass preparation to assess changes in systemic vascular resistance and venous capacitance during infusion of PPF and other possibly vasoactive mediators. Plasma protein fraction caused consistent vasodilation, whereas purified human albumin did not. This vasodilation could be ascribed entirely to acetate, present in PPF as a buffer. Bradykinin in PPF had no effect during venous infusion. Acetate is used widely as a buffer in intravenous and dialysate solutions. Its vasoactive properties must be recognized when such solutions are administered to patients with limited capacity to compensate for sudden vasodilation.", "contents": "Vasodilator effects of the sodium acetate in pooled protein fraction. Paradoxical hypotension during rapid infusion of plasma protein fraction (PPF) has been attributed to vasodilation by bradykinin in PPF. This study employed a canine, controlled right heart bypass preparation to assess changes in systemic vascular resistance and venous capacitance during infusion of PPF and other possibly vasoactive mediators. Plasma protein fraction caused consistent vasodilation, whereas purified human albumin did not. This vasodilation could be ascribed entirely to acetate, present in PPF as a buffer. Bradykinin in PPF had no effect during venous infusion. Acetate is used widely as a buffer in intravenous and dialysate solutions. Its vasoactive properties must be recognized when such solutions are administered to patients with limited capacity to compensate for sudden vasodilation."}
{"id": "PMID:485605", "title": "Surgical treatment of chronic pancreatitis. Twenty-two years' experience.", "content": "Seventy-four patients underwent operation for chronic pancreatitis during a 22 year period at UCLA Hospital. Follow-up data obtained for 60% of these patients an average of 3.2 years postoperation were analyzed by computer for statistically significant benefit between paired operation combinations and the variables of pain relief, stool habits, alcohol use, readmission for pancreatitis, and narcotic use. The combined group of total and cephalic pancreaticoduodenectomy proved more effective with respect to pain relief and readmission (p less than 0.05) than the group that had pseudocyst drainage. The comparison of groups that underwent resection or ductal drainage showed no statistical differences for the above variables. Regardless of type of operation, if the patient had evidence of pancreatic calcifications and had abstained from alcohol postoperatively, the likelihood of a return to normal activity was more favorable (p less than 0.05).", "contents": "Surgical treatment of chronic pancreatitis. Twenty-two years' experience. Seventy-four patients underwent operation for chronic pancreatitis during a 22 year period at UCLA Hospital. Follow-up data obtained for 60% of these patients an average of 3.2 years postoperation were analyzed by computer for statistically significant benefit between paired operation combinations and the variables of pain relief, stool habits, alcohol use, readmission for pancreatitis, and narcotic use. The combined group of total and cephalic pancreaticoduodenectomy proved more effective with respect to pain relief and readmission (p less than 0.05) than the group that had pseudocyst drainage. The comparison of groups that underwent resection or ductal drainage showed no statistical differences for the above variables. Regardless of type of operation, if the patient had evidence of pancreatic calcifications and had abstained from alcohol postoperatively, the likelihood of a return to normal activity was more favorable (p less than 0.05)."}
{"id": "PMID:485606", "title": "Twenty year experience in maxillocraniofacial surgery. An evaluation of early surgery on growth, function and body image.", "content": "We have analyzed 404 patients with a wide variety of maxillary and craniofacial deformities. These do not include head and neck cancer patients as generally defined. Satisfaction has been high as judged by the patients, surgeons and psychiatrists. The complication rate has been significant, approximately 30%, the most common being infection or loss of bone grafts. As experience accumulated, the concept of earlier operative intervention has emerged as an aid in unlocking growth potential, diminishing secondary deformity and improving the development of body image.", "contents": "Twenty year experience in maxillocraniofacial surgery. An evaluation of early surgery on growth, function and body image. We have analyzed 404 patients with a wide variety of maxillary and craniofacial deformities. These do not include head and neck cancer patients as generally defined. Satisfaction has been high as judged by the patients, surgeons and psychiatrists. The complication rate has been significant, approximately 30%, the most common being infection or loss of bone grafts. As experience accumulated, the concept of earlier operative intervention has emerged as an aid in unlocking growth potential, diminishing secondary deformity and improving the development of body image."}
{"id": "PMID:485607", "title": "Congenital diaphragmatic hernia. New concept in management.", "content": "The newborn infant with a congenital diaphragmatic hernia (CDH) who develops severe respiratory distress requiring operative repair within the first 24 hours of life represents one of the most challenging problems in pediatric surgery. The mortality in these infants still exceeds 50% and has historically been attributed to ventilatory insufficiency secondary to pulmonary hypoplasia. However, the primary abnormality in these neonates seems to be an increase in pulmonary vascular resistance with an elevation of pulmonary artery pressure, right-left ductal shunting, preductal shunting and progressive hypoxemia. Eighteen neonates with a CDH were operated upon within the first 24 hours of life with a mortality of 38%. In no instance did ventilatory insufficiency seem to be a major factor in the death of the patient. Seven infants with progressive hypoxemia were treated with a vasodilator, tolazoline. Six of the seven infants showed an initial response to treatment, with a rise in preductal PaO2 and a decrease in ductal shunting. Four of these seven desperately ill neonates survived. Pathologic examination of the pulmonary vasculature in the non-survivors revealed an increase in muscle mass within the pulmonary arterioles. An exaggerated vasoconstrictive response of an abnormally hypertrophied pulmonary vascular bed leading to an elevation of pulmonary vascular resistance, rather than abnormalities of ventilation, appears to be the important mechanism leading to the often fatal hypoxemia observed in the neonate with a CDH. Improved survival will depend upon the successful management of the deranged pulmonary vascular hemodynamics seen in these infants.", "contents": "Congenital diaphragmatic hernia. New concept in management. The newborn infant with a congenital diaphragmatic hernia (CDH) who develops severe respiratory distress requiring operative repair within the first 24 hours of life represents one of the most challenging problems in pediatric surgery. The mortality in these infants still exceeds 50% and has historically been attributed to ventilatory insufficiency secondary to pulmonary hypoplasia. However, the primary abnormality in these neonates seems to be an increase in pulmonary vascular resistance with an elevation of pulmonary artery pressure, right-left ductal shunting, preductal shunting and progressive hypoxemia. Eighteen neonates with a CDH were operated upon within the first 24 hours of life with a mortality of 38%. In no instance did ventilatory insufficiency seem to be a major factor in the death of the patient. Seven infants with progressive hypoxemia were treated with a vasodilator, tolazoline. Six of the seven infants showed an initial response to treatment, with a rise in preductal PaO2 and a decrease in ductal shunting. Four of these seven desperately ill neonates survived. Pathologic examination of the pulmonary vasculature in the non-survivors revealed an increase in muscle mass within the pulmonary arterioles. An exaggerated vasoconstrictive response of an abnormally hypertrophied pulmonary vascular bed leading to an elevation of pulmonary vascular resistance, rather than abnormalities of ventilation, appears to be the important mechanism leading to the often fatal hypoxemia observed in the neonate with a CDH. Improved survival will depend upon the successful management of the deranged pulmonary vascular hemodynamics seen in these infants."}
{"id": "PMID:485608", "title": "The fibrinolytic system. A key to tumor metastasis?", "content": "Neither histologic nor clinical staging reliably correlates with patient survival or the time course of tumor metastatic spread. There is no general biologic tumor marker which is able to distinguish those patients with microscopic residual cancer who may benefit from adjuvant anticancer treatment from those patients cured by their primary treatment who do not require additional anticancer therapy. Our data suggest that tumor activation and inhibition of fibrinolysis are related to the likelihood of tumor spread. Calculation of mean activation/inhibition ratios (A/I ratios) in groups of tumors with and without metastatic spread demonstrated a statistically significant difference between their respective A/I ratios (p less than 0.001). In addition, the mean activation/inhibition ratios for secondary or \"metastatic\" lesions were significantly different from the mean activation/inhibition ratios of the original tumors from which they metastasized (p less than 0.001). Therefore, tumor activation/inhibition ratios would appear to have clinical reliability as biologic markers for the presence or absence of tumor metastases. These data may have important therapeutic implications that would permit the use of activation/inhibition ratios as a biologic marker for the presence or absence of tumor spread at the time of primary surgical excision of the tumor. These observations warrant further investigation into the mechanisms of tumor interaction with the fibrinolytic system.", "contents": "The fibrinolytic system. A key to tumor metastasis? Neither histologic nor clinical staging reliably correlates with patient survival or the time course of tumor metastatic spread. There is no general biologic tumor marker which is able to distinguish those patients with microscopic residual cancer who may benefit from adjuvant anticancer treatment from those patients cured by their primary treatment who do not require additional anticancer therapy. Our data suggest that tumor activation and inhibition of fibrinolysis are related to the likelihood of tumor spread. Calculation of mean activation/inhibition ratios (A/I ratios) in groups of tumors with and without metastatic spread demonstrated a statistically significant difference between their respective A/I ratios (p less than 0.001). In addition, the mean activation/inhibition ratios for secondary or \"metastatic\" lesions were significantly different from the mean activation/inhibition ratios of the original tumors from which they metastasized (p less than 0.001). Therefore, tumor activation/inhibition ratios would appear to have clinical reliability as biologic markers for the presence or absence of tumor metastases. These data may have important therapeutic implications that would permit the use of activation/inhibition ratios as a biologic marker for the presence or absence of tumor spread at the time of primary surgical excision of the tumor. These observations warrant further investigation into the mechanisms of tumor interaction with the fibrinolytic system."}
{"id": "PMID:485609", "title": "Enhanced survival of patients with colon and rectal cancer is based upon wide anatomic resection.", "content": "From 1966 through 1970 we performed resections in 216 patients with carcinoma of the large bowel. The relative five year survival for all patients was 65.5%. The relative five year survival for all potentially curable patients was 80.4%. Patients with positive lymph nodes and full-thickness penetration of their tumors had a five year survival of 70.5% and a 10 year survival of 60.5%. In performing this study we have tested the principles of wide anatomical resection and radical lymphadenectomy. For their specific influences on survival we have also examined stage, site, age, sex, race, margins, local recurrence, hypogastric lymph node dissection, serosal penetration and various aspects of nodal status. The information derived from these parameters has confirmed our hypothesis that survival is directly related to radical anatomical resection and lymphadenectomy. For rectal cancer, extensive resection also reduces the incidence of local recurrence. We are persuaded that the principles of operation for large-bowel cancer are valid and that they merit universal adoption.", "contents": "Enhanced survival of patients with colon and rectal cancer is based upon wide anatomic resection. From 1966 through 1970 we performed resections in 216 patients with carcinoma of the large bowel. The relative five year survival for all patients was 65.5%. The relative five year survival for all potentially curable patients was 80.4%. Patients with positive lymph nodes and full-thickness penetration of their tumors had a five year survival of 70.5% and a 10 year survival of 60.5%. In performing this study we have tested the principles of wide anatomical resection and radical lymphadenectomy. For their specific influences on survival we have also examined stage, site, age, sex, race, margins, local recurrence, hypogastric lymph node dissection, serosal penetration and various aspects of nodal status. The information derived from these parameters has confirmed our hypothesis that survival is directly related to radical anatomical resection and lymphadenectomy. For rectal cancer, extensive resection also reduces the incidence of local recurrence. We are persuaded that the principles of operation for large-bowel cancer are valid and that they merit universal adoption."}
{"id": "PMID:485612", "title": "Histopathology in the evaluation of total pancreatectomy for ductal carcinoma.", "content": "Whipple resections for pancreatic head carcinoma are often inadequate because tumor is left behind in the body and tail. Thirty-six patients have undergone total pancreatectomy for various conditions, of which 25 have undergone total pancreatectomy, for ductal carcinoma. Thirty-seven per cent of these 25 patients have shown histologic evidence that a Whipple resection would not have adequately removed tumor-bearing pancreatic tissue. Three patients had carcinoma spreading up and along the common bile duct from a primary ductal carcinoma in the head of the pancreas. Four patients had tumor infiltrating in continuity into the pancreatic body and tail at a distance from the palpable tumore in the head well to the left of a Whipple transection site. Five patients had widespread multifocal autonomous tumor involving other areas in the gland but with tumor palpable only in the head of the pancreas. Three patients (12%) died postoperatively. The two year survival rate is 32%, and the five year survival, 19%. Histological factors affecting the survival prognosis include 1) positive nodes, 2) tumor extension up the common duct, and 3) intrapancreatic extension and multicentricity of tumor mandating total pancreatectomy for hope of cure in at least 38% of cases.", "contents": "Histopathology in the evaluation of total pancreatectomy for ductal carcinoma. Whipple resections for pancreatic head carcinoma are often inadequate because tumor is left behind in the body and tail. Thirty-six patients have undergone total pancreatectomy for various conditions, of which 25 have undergone total pancreatectomy, for ductal carcinoma. Thirty-seven per cent of these 25 patients have shown histologic evidence that a Whipple resection would not have adequately removed tumor-bearing pancreatic tissue. Three patients had carcinoma spreading up and along the common bile duct from a primary ductal carcinoma in the head of the pancreas. Four patients had tumor infiltrating in continuity into the pancreatic body and tail at a distance from the palpable tumore in the head well to the left of a Whipple transection site. Five patients had widespread multifocal autonomous tumor involving other areas in the gland but with tumor palpable only in the head of the pancreas. Three patients (12%) died postoperatively. The two year survival rate is 32%, and the five year survival, 19%. Histological factors affecting the survival prognosis include 1) positive nodes, 2) tumor extension up the common duct, and 3) intrapancreatic extension and multicentricity of tumor mandating total pancreatectomy for hope of cure in at least 38% of cases."}
{"id": "PMID:485613", "title": "The price of weight loss by jejunoileal shunt.", "content": "In the performance of end-to-end jejunoileal shunt, operative mortality can be nearly eliminated and late deaths largely prevented by assiduous care and follow-up. We attempted to prevent serious complications by regular outpatient visits. However, 703 outpatient visits costing $49.00 per visit failed to improve results. There were 170 readmissions among 64 patients lasting 4--57 days (average hospital stay--16 days per admission at $3,000.00). Twenty-four of those patients alive and followed 18 months or more (53%) sustained adequate weight loss and were free of major problems. Patient satisfaction nevertheless appears high, and when there has been a good weight loss, even severe problems tend to be glossed over by the patient. The ultimate outcome is still unknown, but it seems clear that many of the patients are in a state of controlled malnutrition, which may lead to progressive penalties. We have documented gross pathologic lesions in the bypass enteritis syndrome and draw attention to neurologic sequelae of the bypass, which probably represents deficiency manifestations. Despite brilliant results in some patients and satisfactory results in perhaps half, the cost in life, suffering, dollars, patient and physician time, the uncertain long-term effects, and the unpredictability of the weight loss, all place in question the appropriateness of jejunoileal shunt as the remedy for morbid obesity.", "contents": "The price of weight loss by jejunoileal shunt. In the performance of end-to-end jejunoileal shunt, operative mortality can be nearly eliminated and late deaths largely prevented by assiduous care and follow-up. We attempted to prevent serious complications by regular outpatient visits. However, 703 outpatient visits costing $49.00 per visit failed to improve results. There were 170 readmissions among 64 patients lasting 4--57 days (average hospital stay--16 days per admission at $3,000.00). Twenty-four of those patients alive and followed 18 months or more (53%) sustained adequate weight loss and were free of major problems. Patient satisfaction nevertheless appears high, and when there has been a good weight loss, even severe problems tend to be glossed over by the patient. The ultimate outcome is still unknown, but it seems clear that many of the patients are in a state of controlled malnutrition, which may lead to progressive penalties. We have documented gross pathologic lesions in the bypass enteritis syndrome and draw attention to neurologic sequelae of the bypass, which probably represents deficiency manifestations. Despite brilliant results in some patients and satisfactory results in perhaps half, the cost in life, suffering, dollars, patient and physician time, the uncertain long-term effects, and the unpredictability of the weight loss, all place in question the appropriateness of jejunoileal shunt as the remedy for morbid obesity."}
{"id": "PMID:485614", "title": "Gastric partitioning for morbid obesity.", "content": "The complication rate in jejunoileal bypass for morbid obesity is unacceptably high. Gastric bypass is technically difficult. In our series, 115 patients have undergone gastric partitioning for morbid obesity. The operation consists of stapling across the stomach below the gastroesophageal junction, leaving a gastric food reservoir of 50--60 cc. A 1 cm opening is left in the central portion of the staple line, allowing slow emptying into the distal stomach. The result is a reduced eating capacity and frequency which produce loss in weight. Three-quarters of the patients are women, and the age range is 17--62 years. Preoperative weights averaged 147 kg. Mean operative time was 48 minutes, and postoperative stay was 6.2 days. All patients were extensively evaluated preoperatively with upper GI series, cholecystogram, a number of blood chemistry tests, and endocrinologic and psychiatric consultations. All patients underwent a preoperative Minnesota Multiphasic Personality Inventory test. Cholecystectomy for cholelithiasis was performed on 18% of the patients at the time of operation. Of the seven patients operated on more than one year ago, five have lost an average of 31.6% of their preoperative weight. Of the 12 operated on less than one year but more than six months ago, eight have lost an average of 21% of their initial weight. The early failure rate of 33% has been reduced to 15% at present. One death occurred from pulmonary embolus 10 days following discharge, giving a mortality rate of .08%. The complication rate is 10%, comprising two pulmonary emboli, two psychoses, one wound dehiscence, one wound hernia, and ten wound infections, six of which were minor. There have been no complications of ulcer disease, reflux esophagitis, liver disease, renal disease, or metabolic disorders. Gastric partitioning is a safe, fast effective alternative for the surgical treatment of morbid obesity.", "contents": "Gastric partitioning for morbid obesity. The complication rate in jejunoileal bypass for morbid obesity is unacceptably high. Gastric bypass is technically difficult. In our series, 115 patients have undergone gastric partitioning for morbid obesity. The operation consists of stapling across the stomach below the gastroesophageal junction, leaving a gastric food reservoir of 50--60 cc. A 1 cm opening is left in the central portion of the staple line, allowing slow emptying into the distal stomach. The result is a reduced eating capacity and frequency which produce loss in weight. Three-quarters of the patients are women, and the age range is 17--62 years. Preoperative weights averaged 147 kg. Mean operative time was 48 minutes, and postoperative stay was 6.2 days. All patients were extensively evaluated preoperatively with upper GI series, cholecystogram, a number of blood chemistry tests, and endocrinologic and psychiatric consultations. All patients underwent a preoperative Minnesota Multiphasic Personality Inventory test. Cholecystectomy for cholelithiasis was performed on 18% of the patients at the time of operation. Of the seven patients operated on more than one year ago, five have lost an average of 31.6% of their preoperative weight. Of the 12 operated on less than one year but more than six months ago, eight have lost an average of 21% of their initial weight. The early failure rate of 33% has been reduced to 15% at present. One death occurred from pulmonary embolus 10 days following discharge, giving a mortality rate of .08%. The complication rate is 10%, comprising two pulmonary emboli, two psychoses, one wound dehiscence, one wound hernia, and ten wound infections, six of which were minor. There have been no complications of ulcer disease, reflux esophagitis, liver disease, renal disease, or metabolic disorders. Gastric partitioning is a safe, fast effective alternative for the surgical treatment of morbid obesity."}
{"id": "PMID:485615", "title": "The natural history and surgical significance of hyperlipemic abdominal crisis.", "content": "Although it is widely known that patients with severe hyperlipemia may have pancreatitis, it is not generally appreciated that such patients may have recurrent abdominal pain of variable character and intensity not due to pancreatitis. Review of 35 patients followed in our clinic for 1--11 years showed that 54% had recurrent abdominal pain, while only 29% had pancreatitis. Although mild pain occurred frequently with plasma triglycerides in the 2000--5000 mg/dl range, triglycerides over 6000 mg/dl were often associated with severe pain and physical findings which necessitated hospitalization, often led to the misdiagnosis of pancreatitis and other intra-abdominal catastrophes and resulted in multiple unnecessary diagnostic studies and operations. When recognized, the pain subsided within 48 hours upon cessation of oral intake and treatment with intravenous electrolyte solutions. Furthermore, effective treatment of the hyperlipemia prevented both the attacks of severe pain and the pancreatitis which otherwise occurred (or recurred) in a significant fraction of the patients. These data confirm the existence of hyperlipemic abdominal crisis as a distinct entity and testify to the importance of recognizing this syndrome in order to avoid the occurrence of acute pancreatitis and the performance of unnecessary and potentially harmful surgery.", "contents": "The natural history and surgical significance of hyperlipemic abdominal crisis. Although it is widely known that patients with severe hyperlipemia may have pancreatitis, it is not generally appreciated that such patients may have recurrent abdominal pain of variable character and intensity not due to pancreatitis. Review of 35 patients followed in our clinic for 1--11 years showed that 54% had recurrent abdominal pain, while only 29% had pancreatitis. Although mild pain occurred frequently with plasma triglycerides in the 2000--5000 mg/dl range, triglycerides over 6000 mg/dl were often associated with severe pain and physical findings which necessitated hospitalization, often led to the misdiagnosis of pancreatitis and other intra-abdominal catastrophes and resulted in multiple unnecessary diagnostic studies and operations. When recognized, the pain subsided within 48 hours upon cessation of oral intake and treatment with intravenous electrolyte solutions. Furthermore, effective treatment of the hyperlipemia prevented both the attacks of severe pain and the pancreatitis which otherwise occurred (or recurred) in a significant fraction of the patients. These data confirm the existence of hyperlipemic abdominal crisis as a distinct entity and testify to the importance of recognizing this syndrome in order to avoid the occurrence of acute pancreatitis and the performance of unnecessary and potentially harmful surgery."}
{"id": "PMID:485616", "title": "Surgical decision making. The reliability of clinical judgment.", "content": "Elective surgery second opinion programs are predicted on strict acceptance of the accuracy of the consultant's surgical judgment. The reliability and reproducibility of clinical judgment, therefore, become basic to the effectiveness of such programs. This aspect, however, has received little attention. We report a randomized and controlled survey of surgical specialists which defines agreement/disagreement patterns in surgical decision-making for seven elective surgical procedures. For each disease process, four case histories, including at least one control, were developed by specialty panels of physicians. The case summaries described fictional patients who were seeking professional consultation. The histories were mailed to a random sample of Board-certified specialists from the State of Maryland and the District of Columbia. The response rate was approximately 80% for all five specialties. The respondents were asked to indicate whether they would (Yes) or would not (No) perform the surgical procedure in question. Factual knowledge was not sought, but instead the application of that knowledge and experience to decide on the need for surgical intervention. By comparing the responses for each case history, the agreement/disagreement patterns of inter-observer surgical judgment were determined. Analysis of the data revealed a marked divergence of opinion concerning the need for surgery. The significant point of this study is that surgical judgment differs to a major degree from one surgeon to the next. In a second-opinion program the number of consultants needed to provide a reliable clinical decision probably exceeds the number who are logistically available and that the patient would be willing to visit. Surgical decision-making is a semi-exact scientific process, and it is unreasonable to expect exact answers to clinical problems.", "contents": "Surgical decision making. The reliability of clinical judgment. Elective surgery second opinion programs are predicted on strict acceptance of the accuracy of the consultant's surgical judgment. The reliability and reproducibility of clinical judgment, therefore, become basic to the effectiveness of such programs. This aspect, however, has received little attention. We report a randomized and controlled survey of surgical specialists which defines agreement/disagreement patterns in surgical decision-making for seven elective surgical procedures. For each disease process, four case histories, including at least one control, were developed by specialty panels of physicians. The case summaries described fictional patients who were seeking professional consultation. The histories were mailed to a random sample of Board-certified specialists from the State of Maryland and the District of Columbia. The response rate was approximately 80% for all five specialties. The respondents were asked to indicate whether they would (Yes) or would not (No) perform the surgical procedure in question. Factual knowledge was not sought, but instead the application of that knowledge and experience to decide on the need for surgical intervention. By comparing the responses for each case history, the agreement/disagreement patterns of inter-observer surgical judgment were determined. Analysis of the data revealed a marked divergence of opinion concerning the need for surgery. The significant point of this study is that surgical judgment differs to a major degree from one surgeon to the next. In a second-opinion program the number of consultants needed to provide a reliable clinical decision probably exceeds the number who are logistically available and that the patient would be willing to visit. Surgical decision-making is a semi-exact scientific process, and it is unreasonable to expect exact answers to clinical problems."}
{"id": "PMID:485617", "title": "Recent concepts in the treatment of hepatic trauma: facts and fallacies.", "content": "Experience with 85 consecutive patients treated at Bellevue Hospital for hepatic trauma over the past two years has established the importance of several principles of management. Simple liver injuries can be treated by superficial suture and drainage. Using this approach in 57 patients there were no deaths and no postoperative abscesses. Among 28 other patients with complex liver injuries, the first six patients (Group 1) were treated by lobectomy alone (1 patient), lobectomy and intracaval shunt (3 patients), hepatic artery ligation (1 patient), and left lateral segmentectomy (1 patient). Only one of the six survived. In the next 22 consecutive patients managed by the Pringle maneuver combined with finger fracture technique of the hepatic parenchyma and a viable omental pack there was only one death (4.5%). An intracaval shunt was used successfully once in this group, in a patient with a lacerated middle hepatic vein. Only one patient developed a postoperative subphrenic abscess (4.5%), and no patients required reoperation for bleeding. Eighty-two per cent of these 22 patients safely tolerated inflow occlusion of greater than 20 minutes with steroid protection. Hepatic artery ligation is superfluous in the majority of liver injuries. In complex injuries involving lobar branches of the portal vein, the retrohepatic cava or hepatic veins hepatic artery ligation is probably ineffective. Hepatic resection is rarely required and carries a prohibitive mortality. The finger fracture technique provides a direct approach to the source of heniorrhage and is probably the procedure of choice.", "contents": "Recent concepts in the treatment of hepatic trauma: facts and fallacies. Experience with 85 consecutive patients treated at Bellevue Hospital for hepatic trauma over the past two years has established the importance of several principles of management. Simple liver injuries can be treated by superficial suture and drainage. Using this approach in 57 patients there were no deaths and no postoperative abscesses. Among 28 other patients with complex liver injuries, the first six patients (Group 1) were treated by lobectomy alone (1 patient), lobectomy and intracaval shunt (3 patients), hepatic artery ligation (1 patient), and left lateral segmentectomy (1 patient). Only one of the six survived. In the next 22 consecutive patients managed by the Pringle maneuver combined with finger fracture technique of the hepatic parenchyma and a viable omental pack there was only one death (4.5%). An intracaval shunt was used successfully once in this group, in a patient with a lacerated middle hepatic vein. Only one patient developed a postoperative subphrenic abscess (4.5%), and no patients required reoperation for bleeding. Eighty-two per cent of these 22 patients safely tolerated inflow occlusion of greater than 20 minutes with steroid protection. Hepatic artery ligation is superfluous in the majority of liver injuries. In complex injuries involving lobar branches of the portal vein, the retrohepatic cava or hepatic veins hepatic artery ligation is probably ineffective. Hepatic resection is rarely required and carries a prohibitive mortality. The finger fracture technique provides a direct approach to the source of heniorrhage and is probably the procedure of choice."}
{"id": "PMID:485618", "title": "Reparative cardiac surgery in infants and small children: Five years experience with profound hypothermia and circulatory arrest.", "content": "A five year experience of profound hypothermia and circulatory arrest in the operative management of severe congenital heart disease in 128 infants and children weighing 10 kg or less is reviewed. Hospital mortality was 13% for the entire series--8% in the last two years. Mortality varied with the defect present rather than with the age at operation, and appeared to decline over the five years. There was no morbidity associated particularly with this technique, and no evidence of permanent neurologic nor intellectual impairment. Total arrest time averaged 55 minutes, was related significantly to the defect being repaired, but was not related to hospital mortality. The results support the idea of definitive early cardiac repair for severely symptomatic infants and young children, rather than surgical palliation. The hypothermic arrest technique is attractive since it allows optimal operating conditions, thus permitting an accurate repair and the consequent improvement in surgical results.", "contents": "Reparative cardiac surgery in infants and small children: Five years experience with profound hypothermia and circulatory arrest. A five year experience of profound hypothermia and circulatory arrest in the operative management of severe congenital heart disease in 128 infants and children weighing 10 kg or less is reviewed. Hospital mortality was 13% for the entire series--8% in the last two years. Mortality varied with the defect present rather than with the age at operation, and appeared to decline over the five years. There was no morbidity associated particularly with this technique, and no evidence of permanent neurologic nor intellectual impairment. Total arrest time averaged 55 minutes, was related significantly to the defect being repaired, but was not related to hospital mortality. The results support the idea of definitive early cardiac repair for severely symptomatic infants and young children, rather than surgical palliation. The hypothermic arrest technique is attractive since it allows optimal operating conditions, thus permitting an accurate repair and the consequent improvement in surgical results."}
{"id": "PMID:485620", "title": "Prophylactic revascularization of the gut.", "content": "Reconstitution of the mesenteric vascular circulation, in our experience, is advisable when advanced occlusive disease is noted on the preoperative arteriogram of patients selected for aortoiliofemoral, renal artery, or aortic aneurysm surgery. A lateral aortogram is mandatory, and the presence of an anastomotic meandering mesenteric artery on frontal arteriogram is especially valuable in signaling significant disease. This is the first report of prophylactic concomitant revascularization of compromised mesenteric vessels during aortic reconstructive procedures on selected patients. It is our opinion that such an approach can be a significant deterrent to subsequent catastrophic bowel infarction from mesenteric arterial occlusive disease.", "contents": "Prophylactic revascularization of the gut. Reconstitution of the mesenteric vascular circulation, in our experience, is advisable when advanced occlusive disease is noted on the preoperative arteriogram of patients selected for aortoiliofemoral, renal artery, or aortic aneurysm surgery. A lateral aortogram is mandatory, and the presence of an anastomotic meandering mesenteric artery on frontal arteriogram is especially valuable in signaling significant disease. This is the first report of prophylactic concomitant revascularization of compromised mesenteric vessels during aortic reconstructive procedures on selected patients. It is our opinion that such an approach can be a significant deterrent to subsequent catastrophic bowel infarction from mesenteric arterial occlusive disease."}
{"id": "PMID:485621", "title": "Improved exposure for excision of rectal carcinomas: initial experiences with a pubic resection technique.", "content": "Preliminary experiences with a transpubic approach for carcinoma of the rectum are described. By excising a wedge of pubic bone and freeing the left lateral attachments of the bladder, the entire length of rectum can be exposed, down to the level of the levator muscles. Dissection of the tumor can be performed under direct vision, even in unfavorable anatomic and pathologic situations. Low rectal anastomoses, at levels of 1--2 cm above the anus, may be performed with greater ease. It is felt that urinary problems should be uncommon and that orthopedic complications should not occur since sacroiliac articulations are not disturbed by retraction.", "contents": "Improved exposure for excision of rectal carcinomas: initial experiences with a pubic resection technique. Preliminary experiences with a transpubic approach for carcinoma of the rectum are described. By excising a wedge of pubic bone and freeing the left lateral attachments of the bladder, the entire length of rectum can be exposed, down to the level of the levator muscles. Dissection of the tumor can be performed under direct vision, even in unfavorable anatomic and pathologic situations. Low rectal anastomoses, at levels of 1--2 cm above the anus, may be performed with greater ease. It is felt that urinary problems should be uncommon and that orthopedic complications should not occur since sacroiliac articulations are not disturbed by retraction."}
{"id": "PMID:485622", "title": "The anatomic basis for the occasional failure of transfemoral balloon catheter thromboembolectomy.", "content": "A Fogarty balloon catheter was advanced from the common femoral artery through the popliteal artery and its branches in 15 cadavers. The catheter passed into the peroneal branch 89% of the time. In all 15 cadavers, the peroneal artery was the direct continuation of the popliteal artery and the arterior tibial and posterior tibial arteries branched off at varying angles from the popliteal. This provides an anatomic explanation for the occasional failure of transfemoral Fogarty catheter embolectomy of the leg. Our study suggests that if the patient's foot does not improve after Fogarty embolectomy, the popliteal artery should be exposed and the catheter directed into the shank arteries using vascular forceps.", "contents": "The anatomic basis for the occasional failure of transfemoral balloon catheter thromboembolectomy. A Fogarty balloon catheter was advanced from the common femoral artery through the popliteal artery and its branches in 15 cadavers. The catheter passed into the peroneal branch 89% of the time. In all 15 cadavers, the peroneal artery was the direct continuation of the popliteal artery and the arterior tibial and posterior tibial arteries branched off at varying angles from the popliteal. This provides an anatomic explanation for the occasional failure of transfemoral Fogarty catheter embolectomy of the leg. Our study suggests that if the patient's foot does not improve after Fogarty embolectomy, the popliteal artery should be exposed and the catheter directed into the shank arteries using vascular forceps."}
{"id": "PMID:485624", "title": "Early and late results of surgical correction of pulmonary artery sling.", "content": "Since 1976, we have operated on 4 children with pulmonary vascular sling. They were 5, 12, 19, and 54 months old. All patients were seen initially with severe stridor. Diagnosis was made by barium swallow in all 4. Each child was operated on through a left thoracotomy; the left pulmonary artery was dissected deep between the trachea and esophagus. Systemic heparinization and microsurgical techniques were used. All patients improved after operation. Radioisotope pulmonary ventilation/perfusion scans were performed 9, 9, 14, and 21 months after operation. Decreased ventilation was noted in the right upper lobe of one scan; the other three ventilation scans were normal. Perfusion scans showed good patency of both pulmonary arteries in all 4 patients. It is concluded that pulmonary vascular slings should be treated surgically as soon as diagnosed. Delayed operation can lead to severe tracheomalacia, as demonstrated in our 54-month-old patient.", "contents": "Early and late results of surgical correction of pulmonary artery sling. Since 1976, we have operated on 4 children with pulmonary vascular sling. They were 5, 12, 19, and 54 months old. All patients were seen initially with severe stridor. Diagnosis was made by barium swallow in all 4. Each child was operated on through a left thoracotomy; the left pulmonary artery was dissected deep between the trachea and esophagus. Systemic heparinization and microsurgical techniques were used. All patients improved after operation. Radioisotope pulmonary ventilation/perfusion scans were performed 9, 9, 14, and 21 months after operation. Decreased ventilation was noted in the right upper lobe of one scan; the other three ventilation scans were normal. Perfusion scans showed good patency of both pulmonary arteries in all 4 patients. It is concluded that pulmonary vascular slings should be treated surgically as soon as diagnosed. Delayed operation can lead to severe tracheomalacia, as demonstrated in our 54-month-old patient."}
{"id": "PMID:485625", "title": "Late right heart reconstruction following repair of tetralogy of Fallot.", "content": "Twenty-two symptomatic patients underwent a total of 28 reoperative procedures after initial surgical repair of tetralogy of Fallot. Sixteen of the patients were considered to have unfavorable anatomy of the right ventricular outflow tract (RVOT) or pulmonary artery at the time of initial repair. Pulmonary or tricuspid valve replacement, or replacement of both valves, utilizing a xenograft bioprosthesis was performed in 1 of the 22 initial repairs, 7 of the 22 first reoperations, and 5 of the 6 second reoperations. Ultimately, 14 patients received transannular RVOT patches. The interval between the first and second reoperations for 6 patients who required 2 late reconstructive procedures was 5.8 years. No operative deaths occurred. There were 2 late deaths (1 sudden and 1 due to aspiration). Actuarial survival probability (+/- standard error of the mean) 16 years after initial repair was 72 +/- 21%. Eighteen of the 20 current survivors in the present series are completely asymptomatic without physical restrictions; the other 2 are considered to be in New York Heart Association Functional Class II. No xenograft bioprosthetic dysfunction has occurred to date, but cumulative valve follow-up is limited (13 patient-years). In selected patients, earlier pulmonary or tricuspid valve replacement or replacement of both of these valves can provide some degree of protection against recurrent deterioration.", "contents": "Late right heart reconstruction following repair of tetralogy of Fallot. Twenty-two symptomatic patients underwent a total of 28 reoperative procedures after initial surgical repair of tetralogy of Fallot. Sixteen of the patients were considered to have unfavorable anatomy of the right ventricular outflow tract (RVOT) or pulmonary artery at the time of initial repair. Pulmonary or tricuspid valve replacement, or replacement of both valves, utilizing a xenograft bioprosthesis was performed in 1 of the 22 initial repairs, 7 of the 22 first reoperations, and 5 of the 6 second reoperations. Ultimately, 14 patients received transannular RVOT patches. The interval between the first and second reoperations for 6 patients who required 2 late reconstructive procedures was 5.8 years. No operative deaths occurred. There were 2 late deaths (1 sudden and 1 due to aspiration). Actuarial survival probability (+/- standard error of the mean) 16 years after initial repair was 72 +/- 21%. Eighteen of the 20 current survivors in the present series are completely asymptomatic without physical restrictions; the other 2 are considered to be in New York Heart Association Functional Class II. No xenograft bioprosthetic dysfunction has occurred to date, but cumulative valve follow-up is limited (13 patient-years). In selected patients, earlier pulmonary or tricuspid valve replacement or replacement of both of these valves can provide some degree of protection against recurrent deterioration."}
{"id": "PMID:485626", "title": "Thymoma: a continuing survey at the Massachusetts General Hospital.", "content": "An analysis is presented of the treatment of 103 patients with thymoma, 56 with myasthenia gravis and 47 without. In a 1966 report on the first 63 patients, it was stated that the presence of myasthenia and the finding of local tumor invasion at operation were ominous prognostic indicators. The augmented series suggests that myasthenia no longer carries this stigma, possibly because of improved methods in its management in patients who have undergone operation.", "contents": "Thymoma: a continuing survey at the Massachusetts General Hospital. An analysis is presented of the treatment of 103 patients with thymoma, 56 with myasthenia gravis and 47 without. In a 1966 report on the first 63 patients, it was stated that the presence of myasthenia and the finding of local tumor invasion at operation were ominous prognostic indicators. The augmented series suggests that myasthenia no longer carries this stigma, possibly because of improved methods in its management in patients who have undergone operation."}
{"id": "PMID:485627", "title": "The role of thymectomy in red cell aplasia.", "content": "Red cell aplasia is an unusual cause of anemia. Fifty percent of all patients with red cell aplasia will have a thymoma. Twenty-five to 30% of those who undergo thymectomy will be cured. Data are presented that suggest that any patient with red cell aplasia should have thymectomy through a median sternotomy. One of 3 such patients who underwent the operation has had complete remission for two years.", "contents": "The role of thymectomy in red cell aplasia. Red cell aplasia is an unusual cause of anemia. Fifty percent of all patients with red cell aplasia will have a thymoma. Twenty-five to 30% of those who undergo thymectomy will be cured. Data are presented that suggest that any patient with red cell aplasia should have thymectomy through a median sternotomy. One of 3 such patients who underwent the operation has had complete remission for two years."}
{"id": "PMID:485628", "title": "Dynamic behavior of prosthetic aortic tissue valves as viewed by high-speed cinematography.", "content": "Using a valve testing apparatus of our own design and with a high-speed (600 to 800 frames per second) 16 mm movie camera, films were made of Hancock porcine, Carpentier-Edwards porcine, and Ionescu-Shiley bovine pericardial valves mounted in the aortic position and cycled under physiological conditions at 72 to 100 beats per minute. Fresh and explanted valves were observed using saline or 36.5% glycerol as the pumping solution. When fresh valves were studied using saline solution as the pumpint fluid, the Hancock and Carpentier-Edwards porcine valves showed high-frequency leaflet vibration, which increased in frequency with higher cycling rates. Abnormal leaflet motion was decreased when glycerol was used as the blood analogue. The Ionescu-Shiley bovine pericardial valve did not show abnormal leaflet motion under these conditions. Conclusions drawn from tissue valve testing studies that use excessively high pulsing rates and pressures (accelerated testing) and saline or water as pumping solutions cannot be transposed to predict the fate of tissue valves in a clinical setting.", "contents": "Dynamic behavior of prosthetic aortic tissue valves as viewed by high-speed cinematography. Using a valve testing apparatus of our own design and with a high-speed (600 to 800 frames per second) 16 mm movie camera, films were made of Hancock porcine, Carpentier-Edwards porcine, and Ionescu-Shiley bovine pericardial valves mounted in the aortic position and cycled under physiological conditions at 72 to 100 beats per minute. Fresh and explanted valves were observed using saline or 36.5% glycerol as the pumping solution. When fresh valves were studied using saline solution as the pumpint fluid, the Hancock and Carpentier-Edwards porcine valves showed high-frequency leaflet vibration, which increased in frequency with higher cycling rates. Abnormal leaflet motion was decreased when glycerol was used as the blood analogue. The Ionescu-Shiley bovine pericardial valve did not show abnormal leaflet motion under these conditions. Conclusions drawn from tissue valve testing studies that use excessively high pulsing rates and pressures (accelerated testing) and saline or water as pumping solutions cannot be transposed to predict the fate of tissue valves in a clinical setting."}
{"id": "PMID:485629", "title": "The importance of hyperkalemia in a cold perfusion solution: a correlative study examining myocardial function, metabolism, tissue gases, and substrates.", "content": "Twenty-four pigs were studied to assess the effect of potassium in a cardioplegic solution on the ability of the swine myocardium to maintain functional and metabolic integrity following induced ischemia. The pigs were evaluated on total and right heart bypass with measurement at normothermia and after a one-hour intervention of stroke volume (SV), coronary blood flow (CBF), myocardial oxygen consumption (MVO2), and lactate extraction. Myocardial tissue gases (PmO2 and PmCO2) were continuously monitored and, at the conclusion of the procedure tissues were analyzed for adenosine triphosphate (ATP). There were five interventions: (1) hypothermic perfusion (28 degrees C) (Group 1); (2) hypothermic ischemia (28 degrees C) (Group 2); and hypothermic ischemia with a cardioplegic solution (nonlactated Ringer's solution, pH 7.4, 4 degrees C) using (3) normokalemia (4 mEq of potassium chloride/L, 300 mOsm/L (Group 3), (4) hyperkalemia (43 mEq of KCl/L, 390 mOsm/L) (Group 4), and (5) normokalemia with increased osmolarity (3.6 mEq of KCl/L, 400 mOsm/L) (Groups 5). A significant decrease in SV and elevation in peak PmCO2 were seen in all groups subjected to ischemia except those protected with hyperkalemic solution. We conclude that the presence of hyperkalemia in a cold root perfusion solution provides better myocardial protection than cold root perfusion alone. Furthermore, potassium arrest appears to be more protective than coronary perfusion at 28 degrees C.", "contents": "The importance of hyperkalemia in a cold perfusion solution: a correlative study examining myocardial function, metabolism, tissue gases, and substrates. Twenty-four pigs were studied to assess the effect of potassium in a cardioplegic solution on the ability of the swine myocardium to maintain functional and metabolic integrity following induced ischemia. The pigs were evaluated on total and right heart bypass with measurement at normothermia and after a one-hour intervention of stroke volume (SV), coronary blood flow (CBF), myocardial oxygen consumption (MVO2), and lactate extraction. Myocardial tissue gases (PmO2 and PmCO2) were continuously monitored and, at the conclusion of the procedure tissues were analyzed for adenosine triphosphate (ATP). There were five interventions: (1) hypothermic perfusion (28 degrees C) (Group 1); (2) hypothermic ischemia (28 degrees C) (Group 2); and hypothermic ischemia with a cardioplegic solution (nonlactated Ringer's solution, pH 7.4, 4 degrees C) using (3) normokalemia (4 mEq of potassium chloride/L, 300 mOsm/L (Group 3), (4) hyperkalemia (43 mEq of KCl/L, 390 mOsm/L) (Group 4), and (5) normokalemia with increased osmolarity (3.6 mEq of KCl/L, 400 mOsm/L) (Groups 5). A significant decrease in SV and elevation in peak PmCO2 were seen in all groups subjected to ischemia except those protected with hyperkalemic solution. We conclude that the presence of hyperkalemia in a cold root perfusion solution provides better myocardial protection than cold root perfusion alone. Furthermore, potassium arrest appears to be more protective than coronary perfusion at 28 degrees C."}
{"id": "PMID:485630", "title": "Repair of interrupted aortic arch and aortopulmonary window in an infant.", "content": "The diagnosis and repair in infancy of interrupted aortic arch and aortopulmonary window is described. Using deep hypothermia and circulatory arrest, aortic continuity was established with a prosthetic graft, which was anastomosed to the aortic orifice of the aortopulmonary window. The pulmonary artery side of the aortopulmonary window was closed directly. Postoperative cardiac catheterization demonstrated a good reconstruction. Previous experience with this rare variety of interrupted aortic arch complex is reviewed.", "contents": "Repair of interrupted aortic arch and aortopulmonary window in an infant. The diagnosis and repair in infancy of interrupted aortic arch and aortopulmonary window is described. Using deep hypothermia and circulatory arrest, aortic continuity was established with a prosthetic graft, which was anastomosed to the aortic orifice of the aortopulmonary window. The pulmonary artery side of the aortopulmonary window was closed directly. Postoperative cardiac catheterization demonstrated a good reconstruction. Previous experience with this rare variety of interrupted aortic arch complex is reviewed."}
{"id": "PMID:485631", "title": "Tracheal agenesis.", "content": "Tracheal agenesis is a rare cause of respiratory distress in the neonatal period. Temporary survival depends on ventilation through the esophagus. Thirty-eight case reports of tracheal agenesis (including one from this institution) have appeared in the literature. In this paper, we present the case reports of our 2 patients and review the literature. Tracheal agenesis is associated with a wide variety of congenital anomalies, the most frequent being ventricular septal defect. A new classification encompassing seven types of tracheal agenesis is described.", "contents": "Tracheal agenesis. Tracheal agenesis is a rare cause of respiratory distress in the neonatal period. Temporary survival depends on ventilation through the esophagus. Thirty-eight case reports of tracheal agenesis (including one from this institution) have appeared in the literature. In this paper, we present the case reports of our 2 patients and review the literature. Tracheal agenesis is associated with a wide variety of congenital anomalies, the most frequent being ventricular septal defect. A new classification encompassing seven types of tracheal agenesis is described."}
{"id": "PMID:485632", "title": "Effect of cyproterone acetate on the structure of the adrenal cortex.", "content": "Adult male C57 BL/6J mice injected with 0.08 mg/g of cyproterone acetate showed an increase in cellular lipid content in the zona fasciculata and zona reticularis of the adrenal cortex. In the perimedullary region, densely staining cells aggregated to form scattered nodules. At the ultrastructural level, in the zona glomerulosa and zona fasciculata the number and size of lipid droplets increased. An apparent decrease in mitochondrial number and size and a noticeable diminution in the amount of smooth endoplasmic reticulum were also noted in the zona fasciculata. It is suggested that cyproterone acetate may have blocked steroid synthesis and increased adrenal cholesterol deposits.", "contents": "Effect of cyproterone acetate on the structure of the adrenal cortex. Adult male C57 BL/6J mice injected with 0.08 mg/g of cyproterone acetate showed an increase in cellular lipid content in the zona fasciculata and zona reticularis of the adrenal cortex. In the perimedullary region, densely staining cells aggregated to form scattered nodules. At the ultrastructural level, in the zona glomerulosa and zona fasciculata the number and size of lipid droplets increased. An apparent decrease in mitochondrial number and size and a noticeable diminution in the amount of smooth endoplasmic reticulum were also noted in the zona fasciculata. It is suggested that cyproterone acetate may have blocked steroid synthesis and increased adrenal cholesterol deposits."}
{"id": "PMID:485633", "title": "Uptake of 3H-L-carnitine by isolated rat epididymal tubules.", "content": "The uptake of radiolabeled L-carnitine has been studied in isolated epididymal tubules from the rat. The uptake of 3H-L-carnitine increases with a temperature coefficient KT of 0.22 nmol carnitine.mg protein-1 in the intermal 22--31 degrees and with a low uptake at 4 degrees C. The uptake of radiolabeled carnitine (as percent) is reduced at high concentrations of L-carnitine, by deoxycarnitine but not by D-carnitine. This uptake mechanism is especially active in the distal caput and corpus segments of the epididymis. Thus, an uptake mechanism for carnitine is present in the epididymal cells which besides the carnitine uptake in spermatozoa is responsible for the dramatic increase in carnitine concentration in cauda epididymis.", "contents": "Uptake of 3H-L-carnitine by isolated rat epididymal tubules. The uptake of radiolabeled L-carnitine has been studied in isolated epididymal tubules from the rat. The uptake of 3H-L-carnitine increases with a temperature coefficient KT of 0.22 nmol carnitine.mg protein-1 in the intermal 22--31 degrees and with a low uptake at 4 degrees C. The uptake of radiolabeled carnitine (as percent) is reduced at high concentrations of L-carnitine, by deoxycarnitine but not by D-carnitine. This uptake mechanism is especially active in the distal caput and corpus segments of the epididymis. Thus, an uptake mechanism for carnitine is present in the epididymal cells which besides the carnitine uptake in spermatozoa is responsible for the dramatic increase in carnitine concentration in cauda epididymis."}
{"id": "PMID:485634", "title": "Effects of estradiol-17 beta on reproduction in adult male rats.", "content": "Estradiol-17 beta (E2-17 beta) treatment induced dose-related reduction of sperm population and eventual azoospermia, atrophy of accessory organs, and suppressed serum LH and testosterone levels. In spite of all these alterations mating behavior was not affected, although the number of implantation sites paralleled the reduction of sperm population, suggesting that the primary detectable response to E2-17 beta treatment is related to the steroidogenic components of the testis and the hypothalamic pituitary axis. The duration of treatment is more critical for the induction of azoospermia in the rat than the total dose of E2-17 beta.", "contents": "Effects of estradiol-17 beta on reproduction in adult male rats. Estradiol-17 beta (E2-17 beta) treatment induced dose-related reduction of sperm population and eventual azoospermia, atrophy of accessory organs, and suppressed serum LH and testosterone levels. In spite of all these alterations mating behavior was not affected, although the number of implantation sites paralleled the reduction of sperm population, suggesting that the primary detectable response to E2-17 beta treatment is related to the steroidogenic components of the testis and the hypothalamic pituitary axis. The duration of treatment is more critical for the induction of azoospermia in the rat than the total dose of E2-17 beta."}
{"id": "PMID:485635", "title": "Effect of aromatizable androgens and estradiol on prolactin secretion in prepuberal male rats.", "content": "Intact and castrated male rats were injected daily for 10 days, beginning at 35 days of age, with either oil or one of the following steroids: testosterone propionate, dihydrotestosterone benzoate, androsterone acetate, androstenedione, androstandiol, or estradiol benzoate. Doses were 200 micrograms/rat/day for all androgens and 0.5 microgram or 2 micrograms/rat/day for estradiol. Significant increments in prolactin levels (fourfold over control values) in intact and castrated males were obtained after testosterone propionate and androstenedione treatment. Dihydrotestosterone, androsterone, and androstandiol did not induce any changes in either intact or castrated rats. Estradiol-treated males showed a four- and sevenfold increment in serum prolactin with the 0.5- and 2-microgram doses, respectively. These results suggest that androgens have a role in the control of prolactin secretion, particularly those that can be aromatized to estrogens by different tissues, including the hypothalamus.", "contents": "Effect of aromatizable androgens and estradiol on prolactin secretion in prepuberal male rats. Intact and castrated male rats were injected daily for 10 days, beginning at 35 days of age, with either oil or one of the following steroids: testosterone propionate, dihydrotestosterone benzoate, androsterone acetate, androstenedione, androstandiol, or estradiol benzoate. Doses were 200 micrograms/rat/day for all androgens and 0.5 microgram or 2 micrograms/rat/day for estradiol. Significant increments in prolactin levels (fourfold over control values) in intact and castrated males were obtained after testosterone propionate and androstenedione treatment. Dihydrotestosterone, androsterone, and androstandiol did not induce any changes in either intact or castrated rats. Estradiol-treated males showed a four- and sevenfold increment in serum prolactin with the 0.5- and 2-microgram doses, respectively. These results suggest that androgens have a role in the control of prolactin secretion, particularly those that can be aromatized to estrogens by different tissues, including the hypothalamus."}
{"id": "PMID:485636", "title": "Human spermatozoa: unidirectional rotation of the tail as indicated by head-to-head agglutinates.", "content": "Rotating head-to-head agglutinates of human spermatozoa were always observed to turn counter-clockwise. The phenomenon seems to suggest a unidirectional rotatory component in the tail movement that may be related to the substructure of the axoneme.", "contents": "Human spermatozoa: unidirectional rotation of the tail as indicated by head-to-head agglutinates. Rotating head-to-head agglutinates of human spermatozoa were always observed to turn counter-clockwise. The phenomenon seems to suggest a unidirectional rotatory component in the tail movement that may be related to the substructure of the axoneme."}
{"id": "PMID:485637", "title": "Spermotoxic properties of IgG fractions from normal rabbit sera.", "content": "The antisperm activities of IgG fractions prepared from normal rabbit sera by three different techniques (ammonium sulphate precipitation, gel filtration, and DEAE-Sephadex absorption) were tested using a cytotoxic assay, and antisperm activity detected in fractions prepared by each of these techniques. The nature of the interactions between sperm and IgG molecules from normal sera, and possible functions of \"natural\" antisperm antibodies, are discussed.", "contents": "Spermotoxic properties of IgG fractions from normal rabbit sera. The antisperm activities of IgG fractions prepared from normal rabbit sera by three different techniques (ammonium sulphate precipitation, gel filtration, and DEAE-Sephadex absorption) were tested using a cytotoxic assay, and antisperm activity detected in fractions prepared by each of these techniques. The nature of the interactions between sperm and IgG molecules from normal sera, and possible functions of \"natural\" antisperm antibodies, are discussed."}
{"id": "PMID:485639", "title": "Proliferation of epithelial cells of vas deferens and epididymis in young adult rabbits.", "content": "Epithelial proliferation of the vas deferens and epididymis was studied in rabbits after flash or continuous labeling with DNA precursor by autoradiography. A high proliferative capacity of the epithelium was found: after 2 days of labeling, 11% of labeled cells were found in the vas deferens and 20% in the ductus epididymidis.", "contents": "Proliferation of epithelial cells of vas deferens and epididymis in young adult rabbits. Epithelial proliferation of the vas deferens and epididymis was studied in rabbits after flash or continuous labeling with DNA precursor by autoradiography. A high proliferative capacity of the epithelium was found: after 2 days of labeling, 11% of labeled cells were found in the vas deferens and 20% in the ductus epididymidis."}
{"id": "PMID:485640", "title": "Fine structure of fetal human testis and epididymis.", "content": "Observations on the structure of the human fetal testis and epididymis at 16 weeks' gestation have been made with the light and electron microscope. The fetal testis is organized into solid cords surrounded by a well-defined cellular investment. The basement membrane is multilaminated with a highly redundant basal lamina. The germ cells rest on thin processes from adjacent Sertoli cells. Intercellular bridges between centrally located germ cells have been observed. The epididymis is remarkably well developed. The tall pseudostratified epithelium lines a discrete duct with a patent lumen. Stereocilia and cilia are seen on apical surface of the principal cells.", "contents": "Fine structure of fetal human testis and epididymis. Observations on the structure of the human fetal testis and epididymis at 16 weeks' gestation have been made with the light and electron microscope. The fetal testis is organized into solid cords surrounded by a well-defined cellular investment. The basement membrane is multilaminated with a highly redundant basal lamina. The germ cells rest on thin processes from adjacent Sertoli cells. Intercellular bridges between centrally located germ cells have been observed. The epididymis is remarkably well developed. The tall pseudostratified epithelium lines a discrete duct with a patent lumen. Stereocilia and cilia are seen on apical surface of the principal cells."}
{"id": "PMID:485641", "title": "Interrelationships between androgens and prostaglandins in the vas deferens of prepubertal rats.", "content": "Prostaglandin (PG) levels in the genital tract of male rats were measured at different ages. The vas deferens contained the highest amounts of both PGE2 and PGF2 alpha in young adult or prepubertal animals. PGE2 content increased considerably with age. PGE2 alpha was high in younger animals but decreased markedly at 35 days of age and remained low thereafter. PGE2 and PGF2 alpha were reduced following bilateral castration or hypophysectomy. Testosterone propionate (TP) prevented the postoperative fall in PG levels in a dose-dependent manner. Differences were noted in the relationship between changes in organ weight or PGE2 and pge2 alpha content, and the dose of TP injected, the operation, the time of treatment, and the PG studied.", "contents": "Interrelationships between androgens and prostaglandins in the vas deferens of prepubertal rats. Prostaglandin (PG) levels in the genital tract of male rats were measured at different ages. The vas deferens contained the highest amounts of both PGE2 and PGF2 alpha in young adult or prepubertal animals. PGE2 content increased considerably with age. PGE2 alpha was high in younger animals but decreased markedly at 35 days of age and remained low thereafter. PGE2 and PGF2 alpha were reduced following bilateral castration or hypophysectomy. Testosterone propionate (TP) prevented the postoperative fall in PG levels in a dose-dependent manner. Differences were noted in the relationship between changes in organ weight or PGE2 and pge2 alpha content, and the dose of TP injected, the operation, the time of treatment, and the PG studied."}
{"id": "PMID:485642", "title": "Rouleau formation and fertility of spermatozoa in guinea pigs treated with cyproterone acetate.", "content": "The effects of cyproterone acetate (CA) on fertility of spermatozoa and rouleau formation were investigated in male guinea pigs. Twelve of 15 matings during CA treatment resulted in pregnancy even when rouleaux were absent in ejaculates, indicating that the rouleau condition is not necessary for the fertilizing ability of guinea pig spermatozoa. Examination of epididymides and vasa deferentia revealed that rouleaux diminished progressively with treatment time and were absent in the excurrent ducts of all males during the eighth and ninth weeks of treatment. Following a latent period after treatment, rouleaux were first noted in a specific epididymal region and were present throughout the distal excurrent ducts and ejaculates by 6 wk posttreatment. This sequence of rouleau loss and reappearance in the excurrent ducts suggests that rouleau formation is dependent on a regional epididymal influence that requires androgens. Spermatogenesis was not arrested; however, seminal vesicle and body weights were reduced in treated males.", "contents": "Rouleau formation and fertility of spermatozoa in guinea pigs treated with cyproterone acetate. The effects of cyproterone acetate (CA) on fertility of spermatozoa and rouleau formation were investigated in male guinea pigs. Twelve of 15 matings during CA treatment resulted in pregnancy even when rouleaux were absent in ejaculates, indicating that the rouleau condition is not necessary for the fertilizing ability of guinea pig spermatozoa. Examination of epididymides and vasa deferentia revealed that rouleaux diminished progressively with treatment time and were absent in the excurrent ducts of all males during the eighth and ninth weeks of treatment. Following a latent period after treatment, rouleaux were first noted in a specific epididymal region and were present throughout the distal excurrent ducts and ejaculates by 6 wk posttreatment. This sequence of rouleau loss and reappearance in the excurrent ducts suggests that rouleau formation is dependent on a regional epididymal influence that requires androgens. Spermatogenesis was not arrested; however, seminal vesicle and body weights were reduced in treated males."}
{"id": "PMID:485643", "title": "Patterns of transport and binding of sex steroids.", "content": "The seminal plasma-sex steroid binding kinetic was evaluated by measuring the amount of labeled hormone extractable after 5 sec to 100 min of incubation. For testosterone in normal subjects, a peak of binding was reached after 30 sec of incubation. In subjects with severe oligoasthenospermia the kinetic pattern for testosterone was different but similar to the binding kinetic between seminal plasma of normal men and estradiol. The phenomena did not occur with frozen plasma samples.", "contents": "Patterns of transport and binding of sex steroids. The seminal plasma-sex steroid binding kinetic was evaluated by measuring the amount of labeled hormone extractable after 5 sec to 100 min of incubation. For testosterone in normal subjects, a peak of binding was reached after 30 sec of incubation. In subjects with severe oligoasthenospermia the kinetic pattern for testosterone was different but similar to the binding kinetic between seminal plasma of normal men and estradiol. The phenomena did not occur with frozen plasma samples."}
{"id": "PMID:485645", "title": "Clomiphene test and clomiphene therapy in idiopathic male infertility.", "content": "Clomiphene citrate, 50 mg/day, was administered to 105 patients with idiopathic azoospermia or oligoasthenospermia. Plasma FSH, LH, testosterone, and in a few cases estradiol were evaluated on day 0 and day 15 of therapy. Twelve other patients with no treatment were assayed in the same way. Statistical analysis demonstrated that, as a group, the infertile male population responds normally to clomiphene administration. On the other hand, three types of individual responses were recorded: complete positive response, dissociated positive response, and negative response. Sixty-nine patients were randomly selected for clomiphene therapy, 50 mg/day for 100 days; however, of 54 who completed the treatment schedule, only 11 showed elevated sperm counts. A study of correlations between hormonal and semen responses to clomiphene indicates that a complete or dissociated endocrine response is not an accurate predictor of possible semen amelioration on long-term therapy; on the other hand, sperm characteristics did not improve in those patients who did not demonstrate a positive hormonal response, mainly testosterone, on the 15th day of therapy.", "contents": "Clomiphene test and clomiphene therapy in idiopathic male infertility. Clomiphene citrate, 50 mg/day, was administered to 105 patients with idiopathic azoospermia or oligoasthenospermia. Plasma FSH, LH, testosterone, and in a few cases estradiol were evaluated on day 0 and day 15 of therapy. Twelve other patients with no treatment were assayed in the same way. Statistical analysis demonstrated that, as a group, the infertile male population responds normally to clomiphene administration. On the other hand, three types of individual responses were recorded: complete positive response, dissociated positive response, and negative response. Sixty-nine patients were randomly selected for clomiphene therapy, 50 mg/day for 100 days; however, of 54 who completed the treatment schedule, only 11 showed elevated sperm counts. A study of correlations between hormonal and semen responses to clomiphene indicates that a complete or dissociated endocrine response is not an accurate predictor of possible semen amelioration on long-term therapy; on the other hand, sperm characteristics did not improve in those patients who did not demonstrate a positive hormonal response, mainly testosterone, on the 15th day of therapy."}
{"id": "PMID:485646", "title": "Sperm-agglutinating antibodies in infertile couples.", "content": "In a selected group of 150 infertile couples, sperm-agglutinating antibodies were examined by a microagglutination technique. Sperm-agglutinating activity was observed in sera from 12 men (8%) and 16 women (11%). Antibodies in seminal plasma were demonstrated in 8 men with correspondingly high serum titers. The type of agglutination in these cases was head to tail. Head-to-head agglutination was found in only 3 men, who all had agglutinating activity in serum only. An abnormal postcoital test was found in 57% of the couples, and 13% of the men and 14% of the women in these couples had agglutinating antibodies. A negative postcoital test was related more often to high than to low titer in female sera.", "contents": "Sperm-agglutinating antibodies in infertile couples. In a selected group of 150 infertile couples, sperm-agglutinating antibodies were examined by a microagglutination technique. Sperm-agglutinating activity was observed in sera from 12 men (8%) and 16 women (11%). Antibodies in seminal plasma were demonstrated in 8 men with correspondingly high serum titers. The type of agglutination in these cases was head to tail. Head-to-head agglutination was found in only 3 men, who all had agglutinating activity in serum only. An abnormal postcoital test was found in 57% of the couples, and 13% of the men and 14% of the women in these couples had agglutinating antibodies. A negative postcoital test was related more often to high than to low titer in female sera."}
{"id": "PMID:485647", "title": "Serum and seminal gonadotropins in normal and infertile men: correlations with sperm count, prolactinemia, and seminal prolactin.", "content": "Serum and seminal gonadotropins were evaluated in 30 normozoospermic and 30 oligozoospermic patients. Mean values of basal serum gonadotropins were higher in the oligozoospermic group than in the normozoospermic group: 3.8 versus 1.8 ng/ml and 3.3 versus 2.2 ng/ml for FSH and LH, respectively. Seminal FSH levels were comparable in the normozoospermic and oligozoospermic groups (0.8 versus 0.8 ng/ml, respectively) and were constantly lower than serum FSH levels in both groups. Seminal LH values were constantly higher than serum LH values in the normozoospermic group, whereas in the oligozoospermic group seminal LH values were higher, similar, or lower than serum LH values. However, no significant difference was noted between the two groups for seminal LH values: 3.6 (normozoospermic group) versus 4.1 ng/ml (oligozoospermic group).", "contents": "Serum and seminal gonadotropins in normal and infertile men: correlations with sperm count, prolactinemia, and seminal prolactin. Serum and seminal gonadotropins were evaluated in 30 normozoospermic and 30 oligozoospermic patients. Mean values of basal serum gonadotropins were higher in the oligozoospermic group than in the normozoospermic group: 3.8 versus 1.8 ng/ml and 3.3 versus 2.2 ng/ml for FSH and LH, respectively. Seminal FSH levels were comparable in the normozoospermic and oligozoospermic groups (0.8 versus 0.8 ng/ml, respectively) and were constantly lower than serum FSH levels in both groups. Seminal LH values were constantly higher than serum LH values in the normozoospermic group, whereas in the oligozoospermic group seminal LH values were higher, similar, or lower than serum LH values. However, no significant difference was noted between the two groups for seminal LH values: 3.6 (normozoospermic group) versus 4.1 ng/ml (oligozoospermic group)."}
{"id": "PMID:485648", "title": "Endocrine studies of azoospermia. I. Serum steroid levels in Sertoli cell only syndrome.", "content": "Estrone, estradiol, estriol, progesterone, 17 alpha-hydroxyprogesterone, testosterone, and dihydrotestosterone levels were determined in sera of 20 fertile men and 15 male patients with Sertoli cell only syndrome. In men with Sertoli cel only syndrome there was a significant decrease in serum estrone, estradiol, progesterone, 17 alpha-hydroxyprogesterone, and dihydrotestosterone and a significant increase in serum estriol and testosterone. Changes in endocrine profile discussed in relation to the possible role of inhibin.", "contents": "Endocrine studies of azoospermia. I. Serum steroid levels in Sertoli cell only syndrome. Estrone, estradiol, estriol, progesterone, 17 alpha-hydroxyprogesterone, testosterone, and dihydrotestosterone levels were determined in sera of 20 fertile men and 15 male patients with Sertoli cell only syndrome. In men with Sertoli cel only syndrome there was a significant decrease in serum estrone, estradiol, progesterone, 17 alpha-hydroxyprogesterone, and dihydrotestosterone and a significant increase in serum estriol and testosterone. Changes in endocrine profile discussed in relation to the possible role of inhibin."}
{"id": "PMID:485649", "title": "Free amino acids in semen: measurement and significance in normal and oligozoospermic men.", "content": "Measurement of free amino acids in seminal plasma from fertile and infertile oligozoospermic subjects revealed a quantitative reduction of these molecules in infertile patients. Study of the effect of acidic media on seminal plasma appears to suggest the primary role of free amino acids in the \"protection\" of spermatozoa in the vagina, and the failure of sperm progression in the female genital tract may be due to a decrease of these molecules in semen of infertile patients.", "contents": "Free amino acids in semen: measurement and significance in normal and oligozoospermic men. Measurement of free amino acids in seminal plasma from fertile and infertile oligozoospermic subjects revealed a quantitative reduction of these molecules in infertile patients. Study of the effect of acidic media on seminal plasma appears to suggest the primary role of free amino acids in the \"protection\" of spermatozoa in the vagina, and the failure of sperm progression in the female genital tract may be due to a decrease of these molecules in semen of infertile patients."}
{"id": "PMID:485650", "title": "Aerobic and anaerobic bacterial flora in semen from fertile and infertile groups of men.", "content": "Semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group A) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group B). These 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. Overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group A were positive and 73% from group B were positive. Mixed bacterial flora were isolated from both groups but in group B they were more varied and present in a higher colony count than in group A. Staphylococcus epidermidis, Staph. aureus, Corynebacterium species, Mycoplasma hominis, and Ureaplasma urealyticum were isolated from group A. Group B revealed these organisma plus Streptococcus pneumoniae type III, Strep. pyogenes group A, Strep. feacalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans, Bacteroides species, Peptostreptococcus species, and Eubacterium species. Semen samples from group A were of better quality than those from group B. In addition, the antibacterial effect of seminal plasma from group A was greater than that from group B.", "contents": "Aerobic and anaerobic bacterial flora in semen from fertile and infertile groups of men. Semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group A) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group B). These 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. Overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group A were positive and 73% from group B were positive. Mixed bacterial flora were isolated from both groups but in group B they were more varied and present in a higher colony count than in group A. Staphylococcus epidermidis, Staph. aureus, Corynebacterium species, Mycoplasma hominis, and Ureaplasma urealyticum were isolated from group A. Group B revealed these organisma plus Streptococcus pneumoniae type III, Strep. pyogenes group A, Strep. feacalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans, Bacteroides species, Peptostreptococcus species, and Eubacterium species. Semen samples from group A were of better quality than those from group B. In addition, the antibacterial effect of seminal plasma from group A was greater than that from group B."}
{"id": "PMID:485651", "title": "Male sterility associated with familial translocation heterozygosity: t(8;15) (q22;p11).", "content": "During the investigation of the family of a subject consulting for primary sterility, the same oligoteratospermia was found in two of his brothers. The three karyotypes of these subjects exhibited an equilibrated reciprocal autosomal translocation t(8;15) (q22;p11), which was also detected in their mother. The karyotypes of the remaining siblings, one brother and one sister, were normal. The semen analysis of the sterile subjects suggests that the block of gamete production occurs at the beginning of spermiogenesis. The chromosomal anomaly, which has no effect on the reproduction of the mother, leads to sterility of the male offspring bearing it.", "contents": "Male sterility associated with familial translocation heterozygosity: t(8;15) (q22;p11). During the investigation of the family of a subject consulting for primary sterility, the same oligoteratospermia was found in two of his brothers. The three karyotypes of these subjects exhibited an equilibrated reciprocal autosomal translocation t(8;15) (q22;p11), which was also detected in their mother. The karyotypes of the remaining siblings, one brother and one sister, were normal. The semen analysis of the sterile subjects suggests that the block of gamete production occurs at the beginning of spermiogenesis. The chromosomal anomaly, which has no effect on the reproduction of the mother, leads to sterility of the male offspring bearing it."}
{"id": "PMID:485652", "title": "Experimental comparative study of surgical and needle biopsies of the testis.", "content": "Testis biopsy was performed on 78 rats in order to compare needle and surgical biopsies. The rats were autopsied 7, 15, 30, 60, and 90 days after biopsy. Both techniques provided tissue of equally good quality for histopathological examination and led to irreversible hypospermatogenesis of the biopsied and the contralateral testes.", "contents": "Experimental comparative study of surgical and needle biopsies of the testis. Testis biopsy was performed on 78 rats in order to compare needle and surgical biopsies. The rats were autopsied 7, 15, 30, 60, and 90 days after biopsy. Both techniques provided tissue of equally good quality for histopathological examination and led to irreversible hypospermatogenesis of the biopsied and the contralateral testes."}
{"id": "PMID:485653", "title": "Differences in concanavalin A-FITC binding to rat spermatozoa during epididymal maturation and capacitation.", "content": "Rat spermatozoa from the caput epididymis and cauda epididymis, but not from the testis, demonstrated a crescent of greenish fluorescence on the convex surface of the sperm head, as observed by fluorescence microscopy after staining with concanavalin A-fluorescein isothiocyanate. The percentage of spermatozoa displaying this fluorescent zone decreased during incubation under capacitating conditions, both in vivo and in vitro, suggesting that a glycoprotein zone that is capable of binding concanavalin A was removed or altered during the capacitation period.", "contents": "Differences in concanavalin A-FITC binding to rat spermatozoa during epididymal maturation and capacitation. Rat spermatozoa from the caput epididymis and cauda epididymis, but not from the testis, demonstrated a crescent of greenish fluorescence on the convex surface of the sperm head, as observed by fluorescence microscopy after staining with concanavalin A-fluorescein isothiocyanate. The percentage of spermatozoa displaying this fluorescent zone decreased during incubation under capacitating conditions, both in vivo and in vitro, suggesting that a glycoprotein zone that is capable of binding concanavalin A was removed or altered during the capacitation period."}
{"id": "PMID:485654", "title": "Scanning-electron-microscopy-x-ray microanalysis and distribution of elements within the head of human spermatozoa.", "content": "Scanning electron microscopy (SEM) combined with x-ray analysis has been utilized to investigate the distribution of several elements within the head of human spermatozoon. Despite minor individual variations, a preferential localization of elements (Ca, K, S, P) has been found in the head portion of the cell that corresponds to the post-acrosomal cap and is involved in the fusion of gametes during fertilization.", "contents": "Scanning-electron-microscopy-x-ray microanalysis and distribution of elements within the head of human spermatozoa. Scanning electron microscopy (SEM) combined with x-ray analysis has been utilized to investigate the distribution of several elements within the head of human spermatozoon. Despite minor individual variations, a preferential localization of elements (Ca, K, S, P) has been found in the head portion of the cell that corresponds to the post-acrosomal cap and is involved in the fusion of gametes during fertilization."}
{"id": "PMID:485655", "title": "Testosterone concentration in different functional compartments of the male reproductive tract.", "content": "The concentration of testosterone in testicular tissue (TT), spermatic vein blood (SVB), cubital blood (CB), and seminal fluid (SF) was estimated using a radioimmunoassay technique in a total of 50 normospermic, oligozoospermic, and azoospermic men. Testosterone was also determined in cauda epididymis-vas deferens ductus wash fluid (WF) in 6 normospermic men. In 2 oligozoospermic men the testosterone concentration in TT was 556 and 725 ng/g tissue, respectively. In 9 normospermic men the mean SVB testosterone concentration was 473 ng/ml. CB and SF concentrations of this steroid were 6.8 and 0.4 ng/ml in normospermic, 6.4 and 0.5 ng/ml in oligozoospermic, and 4.9 and 0.4 ng/ml in azoospermic men, respectively; the difference among the three groups was not significant. The SVB:CB and SVB:SF ratios in normospermic men were 71 and 1549, respectively, while the CB:SF ratio was 18 in normospermic, 18 in oligozoospermic, and 16.6 in azoospermic men. Testosterone could not be detected in individual specimens of WF, but after pooling of samples a concentration of 0.08 ng/ml WF was estimated. These results demonstrate the relative concentrations of testosterone in the different functional compartments of the reproductive tract in man and facilitate a better understanding of its function.", "contents": "Testosterone concentration in different functional compartments of the male reproductive tract. The concentration of testosterone in testicular tissue (TT), spermatic vein blood (SVB), cubital blood (CB), and seminal fluid (SF) was estimated using a radioimmunoassay technique in a total of 50 normospermic, oligozoospermic, and azoospermic men. Testosterone was also determined in cauda epididymis-vas deferens ductus wash fluid (WF) in 6 normospermic men. In 2 oligozoospermic men the testosterone concentration in TT was 556 and 725 ng/g tissue, respectively. In 9 normospermic men the mean SVB testosterone concentration was 473 ng/ml. CB and SF concentrations of this steroid were 6.8 and 0.4 ng/ml in normospermic, 6.4 and 0.5 ng/ml in oligozoospermic, and 4.9 and 0.4 ng/ml in azoospermic men, respectively; the difference among the three groups was not significant. The SVB:CB and SVB:SF ratios in normospermic men were 71 and 1549, respectively, while the CB:SF ratio was 18 in normospermic, 18 in oligozoospermic, and 16.6 in azoospermic men. Testosterone could not be detected in individual specimens of WF, but after pooling of samples a concentration of 0.08 ng/ml WF was estimated. These results demonstrate the relative concentrations of testosterone in the different functional compartments of the reproductive tract in man and facilitate a better understanding of its function."}
{"id": "PMID:485656", "title": "Thyroid gland and epididymal function in rats--histological study.", "content": "An experiment was designed to determine the relation between the hypofunction of the thyroid gland and the structure of the epididymis. The hypothyroid condition was associated with decreased mitosis, insufficient vascularization, decreased nuclear size, and chromatin decondensation. In thyroidectomized rats injected with T3, no morphological changes in the epididymis were observed.", "contents": "Thyroid gland and epididymal function in rats--histological study. An experiment was designed to determine the relation between the hypofunction of the thyroid gland and the structure of the epididymis. The hypothyroid condition was associated with decreased mitosis, insufficient vascularization, decreased nuclear size, and chromatin decondensation. In thyroidectomized rats injected with T3, no morphological changes in the epididymis were observed."}
{"id": "PMID:485657", "title": "Binding of 17-beta-estradiol to the outer surface and nucleus of human spermatozoa.", "content": "The binding of 3H-17-beta-estradiol to human ejaculated spermatozoa and to its subcellular structures was studied. The binding kinetics of the labeled steroid to whole spermatozoa followed a parabolic pattern. Scatchard-type plots showed the presence of high-affinity binding sites (1.56 +/- 0.23 X 10(4) per sperm cell) with an apparent Kd of 6.6 X 10(-10) M. In competition experiments testosterone was partially effective in decreasing 17-beta-estradiol binding, whereas progesterone and 17-alpha-estradiol were ineffective. Study of membrane fractions obtained from estradiol-labeled spermatozoa showed that under saturating conditions 75-84% of the bound steroid was bound to sperm membranes. Nuclear fractions obtained from estradiol-labeled spermatozoa showed only 10% of the total bound radioactivity. When isolated sperm nuclei were incubated in the presence of the purified receptor-17-beta-estradiol complex obtained from the high speed supernatant of human uterus almost no transfer of radioactivity to the nuclei was observed.", "contents": "Binding of 17-beta-estradiol to the outer surface and nucleus of human spermatozoa. The binding of 3H-17-beta-estradiol to human ejaculated spermatozoa and to its subcellular structures was studied. The binding kinetics of the labeled steroid to whole spermatozoa followed a parabolic pattern. Scatchard-type plots showed the presence of high-affinity binding sites (1.56 +/- 0.23 X 10(4) per sperm cell) with an apparent Kd of 6.6 X 10(-10) M. In competition experiments testosterone was partially effective in decreasing 17-beta-estradiol binding, whereas progesterone and 17-alpha-estradiol were ineffective. Study of membrane fractions obtained from estradiol-labeled spermatozoa showed that under saturating conditions 75-84% of the bound steroid was bound to sperm membranes. Nuclear fractions obtained from estradiol-labeled spermatozoa showed only 10% of the total bound radioactivity. When isolated sperm nuclei were incubated in the presence of the purified receptor-17-beta-estradiol complex obtained from the high speed supernatant of human uterus almost no transfer of radioactivity to the nuclei was observed."}
{"id": "PMID:485658", "title": "Serum immunoglobulins IgG and IgM in the seminal plasma of men with normospermia, oligozoospermia, and azoospermia.", "content": "The seminal plasma of eight males with normospermia, seven with oligozoospermia, and eight with azoospermia was examined for the presence of IgG and IgM by means of double-gel immunodiffusion. IgG was present in every seminal plasma sample. The highest titers of IgG were found in azoospermic and normospermic samples, but the lowest IgG titers were seen only in normospermic samples. IgM was detected only in some cases, mainly in the azoospermic group. Testicular biopsy was performed in all the azoospermic patients, but IgM was found only in the cases demonstrating spermatogenic arrest and was not found in the cases with complete spermatogenesis. It is concluded that the IgM present in the seminal plasma was of testicular origin and was associated with injury of the male genital system.", "contents": "Serum immunoglobulins IgG and IgM in the seminal plasma of men with normospermia, oligozoospermia, and azoospermia. The seminal plasma of eight males with normospermia, seven with oligozoospermia, and eight with azoospermia was examined for the presence of IgG and IgM by means of double-gel immunodiffusion. IgG was present in every seminal plasma sample. The highest titers of IgG were found in azoospermic and normospermic samples, but the lowest IgG titers were seen only in normospermic samples. IgM was detected only in some cases, mainly in the azoospermic group. Testicular biopsy was performed in all the azoospermic patients, but IgM was found only in the cases demonstrating spermatogenic arrest and was not found in the cases with complete spermatogenesis. It is concluded that the IgM present in the seminal plasma was of testicular origin and was associated with injury of the male genital system."}
{"id": "PMID:485659", "title": "Effects of antigenic competition between sperm autoantigens and ovalbumin upon humoral and cell-mediated immunity and the development of autoimmune aspermatogenic orchitis (AIAO) in guinea pigs.", "content": "The effects of antigenic competition between ovalbumin and sperm autoantigens have been studied in guinea pigs. There was an inhibition of antiovalbumin antibody production up to 60 days after immunization. The cell-mediated immunity against ovalbumin was also depressed at day 30. The simultaneous immunization with both antigens has no effect upon the humoral and cell-mediated immunity against spermatozoa. During the period of inhibition of the humoral and cell-mediated antiovalbumin response, the number of animals developing autoimmune aspermatogenic orchitis was diminished compared to those immunized with spermatozoa alone. Later on, there was no difference between the two groups. The transient inhibition of the immune response against ovalbumin can be explained by the particulate nature of the autoantigens. The sperm cells may be easily trapped by the dendritic reticular cells of the draining lymph nodes. This in turn could affect T cell recognition at early stages, orienting it predominantly toward the sperm autoantigens. At day 90 the situation returned to that present in animals immunized with ovalbumin alone.", "contents": "Effects of antigenic competition between sperm autoantigens and ovalbumin upon humoral and cell-mediated immunity and the development of autoimmune aspermatogenic orchitis (AIAO) in guinea pigs. The effects of antigenic competition between ovalbumin and sperm autoantigens have been studied in guinea pigs. There was an inhibition of antiovalbumin antibody production up to 60 days after immunization. The cell-mediated immunity against ovalbumin was also depressed at day 30. The simultaneous immunization with both antigens has no effect upon the humoral and cell-mediated immunity against spermatozoa. During the period of inhibition of the humoral and cell-mediated antiovalbumin response, the number of animals developing autoimmune aspermatogenic orchitis was diminished compared to those immunized with spermatozoa alone. Later on, there was no difference between the two groups. The transient inhibition of the immune response against ovalbumin can be explained by the particulate nature of the autoantigens. The sperm cells may be easily trapped by the dendritic reticular cells of the draining lymph nodes. This in turn could affect T cell recognition at early stages, orienting it predominantly toward the sperm autoantigens. At day 90 the situation returned to that present in animals immunized with ovalbumin alone."}
{"id": "PMID:485660", "title": "Varicocelectomy in patients with azoospermia.", "content": "The results of surgical treatment of 33 men with azoospermia and varicocele are presented. Microscopic examination of a testicular biospy revealed the Sertoli-cells-only syndrome in nine subjects while degenerative changes in the seminiferous tubules of one or both testes were present in another six persons. In 15 cases decreased spermatogenesis was noted in the testis on the side ipsilateral to the varicocele. In the remaining three subjects the biopsy revealed active spermatogenesis but with absent spermiogenesis. In all cases the changes were equal or more advanced on the affected side. Improvement of the spermiogram was noted in 12 men (34%) and spermatozoa appeared in the ejaculate within 2 to 14 months after operation. In three spouses four gestations occurred. Only in one case where the spermiogram showed improvement were the degenerative changes present in both testes. In the remainder of subjects demonstrating improvement in semen quality, irreversible changes were absent in at least one testis.", "contents": "Varicocelectomy in patients with azoospermia. The results of surgical treatment of 33 men with azoospermia and varicocele are presented. Microscopic examination of a testicular biospy revealed the Sertoli-cells-only syndrome in nine subjects while degenerative changes in the seminiferous tubules of one or both testes were present in another six persons. In 15 cases decreased spermatogenesis was noted in the testis on the side ipsilateral to the varicocele. In the remaining three subjects the biopsy revealed active spermatogenesis but with absent spermiogenesis. In all cases the changes were equal or more advanced on the affected side. Improvement of the spermiogram was noted in 12 men (34%) and spermatozoa appeared in the ejaculate within 2 to 14 months after operation. In three spouses four gestations occurred. Only in one case where the spermiogram showed improvement were the degenerative changes present in both testes. In the remainder of subjects demonstrating improvement in semen quality, irreversible changes were absent in at least one testis."}
{"id": "PMID:485661", "title": "Impaired spermiogenesis as a common finding among professional drivers.", "content": "The effect of long-term driving upon spermiogenesis was investigated in 2984 patients, including 281 professional drivers. The incidence of pathospermia was significantly increased among the 281 occupational drivers as compared to other professionals. The ratio of severe pathospermia was increased in proportion to the number of years of driving. The deterioration of spermiogenesis was mild among car drivers, but was severe in agricultural-industrial hard machinery and farm equipment drivers. There was a higher incidence of impaired fertility in drivers as compared to other professionals. The possible factors influencing these results are discussed.", "contents": "Impaired spermiogenesis as a common finding among professional drivers. The effect of long-term driving upon spermiogenesis was investigated in 2984 patients, including 281 professional drivers. The incidence of pathospermia was significantly increased among the 281 occupational drivers as compared to other professionals. The ratio of severe pathospermia was increased in proportion to the number of years of driving. The deterioration of spermiogenesis was mild among car drivers, but was severe in agricultural-industrial hard machinery and farm equipment drivers. There was a higher incidence of impaired fertility in drivers as compared to other professionals. The possible factors influencing these results are discussed."}
{"id": "PMID:485662", "title": "Effect of alpha-chlorohydrin on epididymal sperm and epididymal plasma in swine.", "content": "Investigation of the contraceptive mechanism of alpha-chlorohydrin was done by analyses of epididymal plasma and certain epididymal sperm characteristics after oral administration of 0, 5, 10 or 30 mg/kg, day of the drug to boars for 15 days. Water resorption in caput epididymidis was slightly decreased in all treatment groups. Sodium, potassium, chloride, glycerylphosphorylcholine levels, and seminal antigens in epididymal plasma were not altered significantly by 10 or 30 mg/kg of the drug. The boars on 5 mg/kg exhibited significantly elevated sodium, potassium, or chloride values in various segments. Motility was significantly lower on corpus and proximal cauda epididymal spermatozoa from alpha-chlorohydrin treated boars. Only boars receiving 30 mg/kg exhibited impaired sperm motility in the distal cauda. The movement of the cytoplasmic droplets to the distal position was retarded in boars on the two highest dose levels. The results suggest that the contraceptive effects of alpha-chlorohydrin in the boar is probably not mediated via an impaired epididymal function.", "contents": "Effect of alpha-chlorohydrin on epididymal sperm and epididymal plasma in swine. Investigation of the contraceptive mechanism of alpha-chlorohydrin was done by analyses of epididymal plasma and certain epididymal sperm characteristics after oral administration of 0, 5, 10 or 30 mg/kg, day of the drug to boars for 15 days. Water resorption in caput epididymidis was slightly decreased in all treatment groups. Sodium, potassium, chloride, glycerylphosphorylcholine levels, and seminal antigens in epididymal plasma were not altered significantly by 10 or 30 mg/kg of the drug. The boars on 5 mg/kg exhibited significantly elevated sodium, potassium, or chloride values in various segments. Motility was significantly lower on corpus and proximal cauda epididymal spermatozoa from alpha-chlorohydrin treated boars. Only boars receiving 30 mg/kg exhibited impaired sperm motility in the distal cauda. The movement of the cytoplasmic droplets to the distal position was retarded in boars on the two highest dose levels. The results suggest that the contraceptive effects of alpha-chlorohydrin in the boar is probably not mediated via an impaired epididymal function."}
{"id": "PMID:485663", "title": "Acid phosphatase activity in human semen.", "content": "Acid phosphatase activity was examined in normal and abnormal human semen of individuals not suffering from testosterone deficiency. No differences in enzymatic activity between the examined groups were observed. The presence of tartaric acid caused an inhibition of about 95% of enzymatic activity.", "contents": "Acid phosphatase activity in human semen. Acid phosphatase activity was examined in normal and abnormal human semen of individuals not suffering from testosterone deficiency. No differences in enzymatic activity between the examined groups were observed. The presence of tartaric acid caused an inhibition of about 95% of enzymatic activity."}
{"id": "PMID:485666", "title": "[Quantification of ventricular volume. Comparative study of 2 angiographic methods].", "content": "The left ventricular volumes and the ejection fraction were measured in 78 patients who had different cardiopathies, in more than 50% the diagnosis of ischaemic heart disease of coronary origin was made. Every cineangiocardiogram was carefully observed to find those who had normal and abnormal ventricular contraction. The ventricular volumes were calculated by the Green method and afterwards the results were compared with the values obtained by the Sandler and Dodge method. A very good correlation was found between the two methods and we got the conclusion that although it is preferable to calculate ventricular volumes and the ejection fraction with biplane method or with Saudler and Dodge's monoplane, the results of the present work permit the use of Green's method in calculating ventricular volumes and obtaining confident results, independently of the ventricular contraction type with the exception of those who have large ventricular aneurisms.", "contents": "[Quantification of ventricular volume. Comparative study of 2 angiographic methods]. The left ventricular volumes and the ejection fraction were measured in 78 patients who had different cardiopathies, in more than 50% the diagnosis of ischaemic heart disease of coronary origin was made. Every cineangiocardiogram was carefully observed to find those who had normal and abnormal ventricular contraction. The ventricular volumes were calculated by the Green method and afterwards the results were compared with the values obtained by the Sandler and Dodge method. A very good correlation was found between the two methods and we got the conclusion that although it is preferable to calculate ventricular volumes and the ejection fraction with biplane method or with Saudler and Dodge's monoplane, the results of the present work permit the use of Green's method in calculating ventricular volumes and obtaining confident results, independently of the ventricular contraction type with the exception of those who have large ventricular aneurisms."}
{"id": "PMID:485667", "title": "[Changes induced by hypertonic solutions in the transportation of calcium by the cardiac reticular sarcoplasma].", "content": "In the sarcoplasmic reticulum of the myocardium, celular organell which function is to regulate the cytoplasmic concentration of calcium in contraction and relaxation, we have studied the effect of hypertonic solutions of sucrose between 1 and 6.96 times the normal tonicity in order to observe the behavior of the internal linked or free calcium of this structure, as well as to prove the hypothesis that hypertonic solutions encourage the calcium exit of the sarcoplasmatic reticulum with the resulting signs of contractures. The following results were obtained: 1. The ATP hydrolisis and calcium transport rate are 14% and 90% respectively of the maximum speeds of 10(-5) M in calcium, while for concentrations of 10(-7) M or ess of the said cation, the transport rates and the ATPase do not reach 5% of the maximum values. 2. Between 1 and 2.54 times of the normal tonicity the calcium uptake remains between 400 and 500 nmoles of calcium/mg protein/min, the transported amount of calcium varies between 14 and 16 nmoles/mg protein and the rate of the ATP hydrolysis increases a 37% to 0.4 M in sucrose. 3. Between 0.4 and 1.2 M in sucrose of 2.54 to 6.96 times the isotonicity, the calcium transport rate velocity as well as the ATP hydrolisis are strongly inhibited. The vesicles volume minimizes and the amount of linked calcium remains within the control values, proving that the capacity of linking this cathion is independent from sarcoplasmic reticulum volume. These results show that the sarcoplasmic reticulum is involved in the contractures induced by hypertonic solutions in intact cells, since the osmolarity increase produces changes of volume which results in a decrease of the calcium transportation velocity or in an increase of the exit of said cathion.", "contents": "[Changes induced by hypertonic solutions in the transportation of calcium by the cardiac reticular sarcoplasma]. In the sarcoplasmic reticulum of the myocardium, celular organell which function is to regulate the cytoplasmic concentration of calcium in contraction and relaxation, we have studied the effect of hypertonic solutions of sucrose between 1 and 6.96 times the normal tonicity in order to observe the behavior of the internal linked or free calcium of this structure, as well as to prove the hypothesis that hypertonic solutions encourage the calcium exit of the sarcoplasmatic reticulum with the resulting signs of contractures. The following results were obtained: 1. The ATP hydrolisis and calcium transport rate are 14% and 90% respectively of the maximum speeds of 10(-5) M in calcium, while for concentrations of 10(-7) M or ess of the said cation, the transport rates and the ATPase do not reach 5% of the maximum values. 2. Between 1 and 2.54 times of the normal tonicity the calcium uptake remains between 400 and 500 nmoles of calcium/mg protein/min, the transported amount of calcium varies between 14 and 16 nmoles/mg protein and the rate of the ATP hydrolysis increases a 37% to 0.4 M in sucrose. 3. Between 0.4 and 1.2 M in sucrose of 2.54 to 6.96 times the isotonicity, the calcium transport rate velocity as well as the ATP hydrolisis are strongly inhibited. The vesicles volume minimizes and the amount of linked calcium remains within the control values, proving that the capacity of linking this cathion is independent from sarcoplasmic reticulum volume. These results show that the sarcoplasmic reticulum is involved in the contractures induced by hypertonic solutions in intact cells, since the osmolarity increase produces changes of volume which results in a decrease of the calcium transportation velocity or in an increase of the exit of said cathion."}
{"id": "PMID:485668", "title": "[Phonomechanocardiographic study of congestive myocardiopathy].", "content": "There were studied 14 patients with congestive myocardiopathy demonstrated by cardiac catheterism at nine, echocardiogramme at five and/or necropsy at four. There were registered mytral insufficiency blowings at thirteen and tricusp insufficiency blowings at five. Of them, there were 11 that presented pathologic noises III and IV. Right apexcardiogramme showed growth of such cavity at the twelve patients that were studied. \"a\" index of the same precordiogramme had qualitative correlation with systolic pulmonary pression. Apexcardiogramme showed ventricular growth at twelve from thirteen patients and \"a\" index was also qualitatively correlated with direct deermination of the left ventricule's telediastolic pression. Chronocardiometry was anormal at all of them. Short expulsive period, long pre-expulsive period, expulsion's fraction diminution reckoned by this method and systolic quotient, all of them diminished, translated the cardiac expense fall by \"pump\" fail. Elongation of pre-isosystolic phase, isosystolic phase, true isosystolic phase, and diminution of ventricular pression's elevation middle velocity and contractility index were consequences of myocardic contraction's bad quality. Decrement of ventricular pression's elevation velocity, added to the important elevation of left ventricle's final diastolic pression determined the \"pseudonormality\" of IIa-0 interval, and of the integrated isovolumetric pression. Shortening of fast filled's phase is explained by a minor ventricular filled in order to the volume's increase and diastolic pression's increase (Board VII). By last, shortening of Q-IIa interval, coinciding with the cardiac frecuence's increase is explained by catecolamins' increased secretion like compensating mechanism of chronic cardiac insufficiency. Phonomechanocardiogramme is useful for entity's diagnostic and it informs about ventricular disfunction which characterise the suffering.", "contents": "[Phonomechanocardiographic study of congestive myocardiopathy]. There were studied 14 patients with congestive myocardiopathy demonstrated by cardiac catheterism at nine, echocardiogramme at five and/or necropsy at four. There were registered mytral insufficiency blowings at thirteen and tricusp insufficiency blowings at five. Of them, there were 11 that presented pathologic noises III and IV. Right apexcardiogramme showed growth of such cavity at the twelve patients that were studied. \"a\" index of the same precordiogramme had qualitative correlation with systolic pulmonary pression. Apexcardiogramme showed ventricular growth at twelve from thirteen patients and \"a\" index was also qualitatively correlated with direct deermination of the left ventricule's telediastolic pression. Chronocardiometry was anormal at all of them. Short expulsive period, long pre-expulsive period, expulsion's fraction diminution reckoned by this method and systolic quotient, all of them diminished, translated the cardiac expense fall by \"pump\" fail. Elongation of pre-isosystolic phase, isosystolic phase, true isosystolic phase, and diminution of ventricular pression's elevation middle velocity and contractility index were consequences of myocardic contraction's bad quality. Decrement of ventricular pression's elevation velocity, added to the important elevation of left ventricle's final diastolic pression determined the \"pseudonormality\" of IIa-0 interval, and of the integrated isovolumetric pression. Shortening of fast filled's phase is explained by a minor ventricular filled in order to the volume's increase and diastolic pression's increase (Board VII). By last, shortening of Q-IIa interval, coinciding with the cardiac frecuence's increase is explained by catecolamins' increased secretion like compensating mechanism of chronic cardiac insufficiency. Phonomechanocardiogramme is useful for entity's diagnostic and it informs about ventricular disfunction which characterise the suffering."}
{"id": "PMID:485669", "title": "[Evaluation of radioactive phosphate scintillography in relation to time elapsed after acute myocardial infarct].", "content": "A consecutive series of 205 myocardial scans, performed with 99mTc-labeled phosphates, in 185 patients with acute chest pain, were independently evaluated by comparing myocardial concentration intensity of the tracer to that in bone, and by rating this intensity by a six-category rating scale, which imply five criterion levels for calling an image \"positive\". The optimal criterion level was determined for each day of evolution of illness, as the one in which a shift to a more lax criterion level produces a bigger increment in false-positive results than in True-positive results, and in which a shift to a stricter criterion level causes a greater decrease in true positive results than in false-negative results. In all instances, the optimal criterion level was that with a moderate (2+) myocardial tracer concentration, lower than rib uptake, but with a focal pattern of myocardial distribution of the tracer (2F). By using this optimal criterion level, our results agree with the general consensus in that the procedure's usefullness is restricted to the first five days of evolution of illness. However, our daily statistical analysis showed that the best period to obtain the maximal diagnostic efficiency lies between the third and fourth days after the onset of acute chest pain. A negative result during this period, in patients with ECG and CPK serum concentration compatible with acute myocardial infarction, should be considered of prognostic significance, since it may translate a defficient colateral coronary circulation.", "contents": "[Evaluation of radioactive phosphate scintillography in relation to time elapsed after acute myocardial infarct]. A consecutive series of 205 myocardial scans, performed with 99mTc-labeled phosphates, in 185 patients with acute chest pain, were independently evaluated by comparing myocardial concentration intensity of the tracer to that in bone, and by rating this intensity by a six-category rating scale, which imply five criterion levels for calling an image \"positive\". The optimal criterion level was determined for each day of evolution of illness, as the one in which a shift to a more lax criterion level produces a bigger increment in false-positive results than in True-positive results, and in which a shift to a stricter criterion level causes a greater decrease in true positive results than in false-negative results. In all instances, the optimal criterion level was that with a moderate (2+) myocardial tracer concentration, lower than rib uptake, but with a focal pattern of myocardial distribution of the tracer (2F). By using this optimal criterion level, our results agree with the general consensus in that the procedure's usefullness is restricted to the first five days of evolution of illness. However, our daily statistical analysis showed that the best period to obtain the maximal diagnostic efficiency lies between the third and fourth days after the onset of acute chest pain. A negative result during this period, in patients with ECG and CPK serum concentration compatible with acute myocardial infarction, should be considered of prognostic significance, since it may translate a defficient colateral coronary circulation."}
{"id": "PMID:485670", "title": "[Clinical, echocardiographic and hemodynamic sequence in cerebral arteriovenous fistula].", "content": "It is presented a fatal case of cerebral arteriovenous tube in which it was obtained clinical, radiological, electric, echocardiographic and haemodinamic sequencial information since the birth. Data suggested biventricular overcharge's existence initially without cardiac insufficiency. Finally appeared right ventricular expansion's data and biventricular hypertrophy, with left ventricular fluxe's diminution and cardiac insufficiency's data and shock.", "contents": "[Clinical, echocardiographic and hemodynamic sequence in cerebral arteriovenous fistula]. It is presented a fatal case of cerebral arteriovenous tube in which it was obtained clinical, radiological, electric, echocardiographic and haemodinamic sequencial information since the birth. Data suggested biventricular overcharge's existence initially without cardiac insufficiency. Finally appeared right ventricular expansion's data and biventricular hypertrophy, with left ventricular fluxe's diminution and cardiac insufficiency's data and shock."}
{"id": "PMID:485671", "title": "[Evaluation of the electrocardiogram in tricuspid atresia].", "content": "Due to the broad behaviour variability of the tricuspid atresia by the defects to which it is associated, the authors propose another classification, based on the position of the great vessels and the characteristics of the pulmonary flow, in addition to the extent of the intracardiac septal defects (atrial septal defect and ventricular septal defect) and the presence of patent ductus arterious. Eleven patients with tricuspid atresia with situs solitus and heart normo-placed are studied for diagnosis confirmation by catheterism or autopsy. Electrocardiograms are studied and observed in two patients with transposition of the great vessels and pulmonary oligohemia, a more evident right auricular enlargement (P of greater voltage in DII and AP heading right in the front plane), the AQRS deviated to the right, up and backwards, with less evidence of the left ventricular power than in other varieties of tricuspid atresia. These characteristics gave a diagnosis useful for this variation. On those patients without transposition and pulmonary flow increase, it is observed that growing appearance of the left auricle is more evident and so is the power of the free wall of the right ventricle (high R in right precordial deviations), the AQRS heads left as in most of the cases, but forward and upwards also. This electrocardiographic information is considered to have great importance to establish a diagnosis of the variations of tricuspid atresia due to associated malformations. Common varieties have already been extensively described by other authors and are not subject to special study. Bibliography on this matter is reviewed.", "contents": "[Evaluation of the electrocardiogram in tricuspid atresia]. Due to the broad behaviour variability of the tricuspid atresia by the defects to which it is associated, the authors propose another classification, based on the position of the great vessels and the characteristics of the pulmonary flow, in addition to the extent of the intracardiac septal defects (atrial septal defect and ventricular septal defect) and the presence of patent ductus arterious. Eleven patients with tricuspid atresia with situs solitus and heart normo-placed are studied for diagnosis confirmation by catheterism or autopsy. Electrocardiograms are studied and observed in two patients with transposition of the great vessels and pulmonary oligohemia, a more evident right auricular enlargement (P of greater voltage in DII and AP heading right in the front plane), the AQRS deviated to the right, up and backwards, with less evidence of the left ventricular power than in other varieties of tricuspid atresia. These characteristics gave a diagnosis useful for this variation. On those patients without transposition and pulmonary flow increase, it is observed that growing appearance of the left auricle is more evident and so is the power of the free wall of the right ventricle (high R in right precordial deviations), the AQRS heads left as in most of the cases, but forward and upwards also. This electrocardiographic information is considered to have great importance to establish a diagnosis of the variations of tricuspid atresia due to associated malformations. Common varieties have already been extensively described by other authors and are not subject to special study. Bibliography on this matter is reviewed."}
{"id": "PMID:485676", "title": "Impairment of cerebral blood flow autoregulation after hypertonic urea administration.", "content": "The effect of unilateral intracarotid administration of 3 M urea on resting cerebral blood flow, arterial blood pressure, perfusion pressure-cerebral blood flow relation, ECoG and Evans blue permeability was tested in dogs. The injection of urea was without effect on ECoG and enhanced the arterial blood pressure temporarily. Cerebral blood flow measured 10 min after the urea injection was not affected. Extravasation of Evans blue was observed in the urea-perfused hemisphere for about 40 min following the urea administration. Cerebral perfusion pressure-blood flow relation for the urea affected hemisphere studied at the time when blood-brain barrier to protein was restored revealed loss of the normal autoregulatory response, despite that it was preserved in the control hemisphere.", "contents": "Impairment of cerebral blood flow autoregulation after hypertonic urea administration. The effect of unilateral intracarotid administration of 3 M urea on resting cerebral blood flow, arterial blood pressure, perfusion pressure-cerebral blood flow relation, ECoG and Evans blue permeability was tested in dogs. The injection of urea was without effect on ECoG and enhanced the arterial blood pressure temporarily. Cerebral blood flow measured 10 min after the urea injection was not affected. Extravasation of Evans blue was observed in the urea-perfused hemisphere for about 40 min following the urea administration. Cerebral perfusion pressure-blood flow relation for the urea affected hemisphere studied at the time when blood-brain barrier to protein was restored revealed loss of the normal autoregulatory response, despite that it was preserved in the control hemisphere."}
{"id": "PMID:485677", "title": "Graded flow reductions and O2 consumption of small partially ischemic region of dog left ventricle.", "content": "The relationship of O2 supply and demand was studied in a small region of a normally functioning left ventricle in 11 pentobarbital anesthetized open chest dogs. A small myocardial vein was catheterized for flow and venous O2 content measurements. An electromagnetic flow probe and screw-type occluder were placed on the supply artery. Arterial flow reductions produced a lesser decrease in venous outflow. This indicated that collateral channels were functional even with relatively small reduction in arterial flow. Even with complete arterial blockage, venous flow never fell below 13.7% of control. Supply had to be reduced to 80.8% of control before we could predict with 95% confidence that O2 demand would fall. Using this technique, it would be possible to test antianginal agents' actions on the O2 supply and demand relation in a region of flow restricted myocardium.", "contents": "Graded flow reductions and O2 consumption of small partially ischemic region of dog left ventricle. The relationship of O2 supply and demand was studied in a small region of a normally functioning left ventricle in 11 pentobarbital anesthetized open chest dogs. A small myocardial vein was catheterized for flow and venous O2 content measurements. An electromagnetic flow probe and screw-type occluder were placed on the supply artery. Arterial flow reductions produced a lesser decrease in venous outflow. This indicated that collateral channels were functional even with relatively small reduction in arterial flow. Even with complete arterial blockage, venous flow never fell below 13.7% of control. Supply had to be reduced to 80.8% of control before we could predict with 95% confidence that O2 demand would fall. Using this technique, it would be possible to test antianginal agents' actions on the O2 supply and demand relation in a region of flow restricted myocardium."}
{"id": "PMID:485678", "title": "The effects of phenytoin on motor function in awake cats.", "content": "Adult cats were monitored for their performance of a variety of motor functions before and after acute administration of phenytoin (5, 10 or 20 mg/kg) in a schedule in which each animal received all drug doses. The only significant loss in motor function was balance and coordination. Half the animals could not balance or walk along a narrow-edged beam after 20 mg/kg of phenytoin although their performance was not impaired at lower drug doses or on wider surfaces. There were no effects of phenytoin on the righting reflex, flexor reflex, muscle strength, the hopping response, the blind placing response or visually aided placing. The data suggest that phenytoin has a selective effect on higher order neuronal systems involved with balance and locomotion rather than simple reflex pathways.", "contents": "The effects of phenytoin on motor function in awake cats. Adult cats were monitored for their performance of a variety of motor functions before and after acute administration of phenytoin (5, 10 or 20 mg/kg) in a schedule in which each animal received all drug doses. The only significant loss in motor function was balance and coordination. Half the animals could not balance or walk along a narrow-edged beam after 20 mg/kg of phenytoin although their performance was not impaired at lower drug doses or on wider surfaces. There were no effects of phenytoin on the righting reflex, flexor reflex, muscle strength, the hopping response, the blind placing response or visually aided placing. The data suggest that phenytoin has a selective effect on higher order neuronal systems involved with balance and locomotion rather than simple reflex pathways."}
{"id": "PMID:485679", "title": "Inhibitory effects of aromatic oligopeptides on the excitability of identifiable giant neurones of an African giant snail (Achatina fulica F\u00e9russac).", "content": "Effects of an aromatic tripeptide, L-Lys-L-Phe-L-Tyr and its related dipeptides on the excitability of two identifiable giant neurones (the TAN, tonically autoactive neurone and the PON, periodically oscillating neurone) in the suboesophageal ganglia of the African giant snail, Achatina fulica F\u00e9russac, were examined. Of 33 substances examined, three aromatic oligopeptides showed a marked inhibitory effect on the TAN excitability. The critical concentrations of these substances in bath application were: L-Lys-LPhe-L-Tyr (6.6 x 10(-6) approximately 2.2 x 10(-5) M), L-Phe-L-Tyr (9.1 x 10(-6) approximately 3.0 x 10(-5) M) and L-Phe-L-Trp (2.4 x 10(-5) approximately 7.3 x 10(-5) M). The microdrop application (100 micron in diameter) of a solution of the three inhibitory oligopeptides on the TAN surface hyperpolarized the TAN membrane potential and eliminated the spike discharges, in both the physiological and the chloride-free (replaced with acetate) conditions. The current-voltage relationships (I-V curve) of the TAN neuromembrane were measured by the injection of a transmembrane triangular current. The TAN I-V curve measured in the presence of L-Lys-L-Phe-L-Tyr or L-Phe-L-Trp was almost concordant to that of the normal state, if the two I-V curves were superimposed using the firing level as the reference point. The same three aromatic oligopeptides also showed an inhibitory effect on the PON excitability. The critical concentrations needed to produce the effects were much higher than those in the case of the TAN.", "contents": "Inhibitory effects of aromatic oligopeptides on the excitability of identifiable giant neurones of an African giant snail (Achatina fulica F\u00e9russac). Effects of an aromatic tripeptide, L-Lys-L-Phe-L-Tyr and its related dipeptides on the excitability of two identifiable giant neurones (the TAN, tonically autoactive neurone and the PON, periodically oscillating neurone) in the suboesophageal ganglia of the African giant snail, Achatina fulica F\u00e9russac, were examined. Of 33 substances examined, three aromatic oligopeptides showed a marked inhibitory effect on the TAN excitability. The critical concentrations of these substances in bath application were: L-Lys-LPhe-L-Tyr (6.6 x 10(-6) approximately 2.2 x 10(-5) M), L-Phe-L-Tyr (9.1 x 10(-6) approximately 3.0 x 10(-5) M) and L-Phe-L-Trp (2.4 x 10(-5) approximately 7.3 x 10(-5) M). The microdrop application (100 micron in diameter) of a solution of the three inhibitory oligopeptides on the TAN surface hyperpolarized the TAN membrane potential and eliminated the spike discharges, in both the physiological and the chloride-free (replaced with acetate) conditions. The current-voltage relationships (I-V curve) of the TAN neuromembrane were measured by the injection of a transmembrane triangular current. The TAN I-V curve measured in the presence of L-Lys-L-Phe-L-Tyr or L-Phe-L-Trp was almost concordant to that of the normal state, if the two I-V curves were superimposed using the firing level as the reference point. The same three aromatic oligopeptides also showed an inhibitory effect on the PON excitability. The critical concentrations needed to produce the effects were much higher than those in the case of the TAN."}
{"id": "PMID:485674", "title": "[Therapeutic efficiency of penicillamine D in rheumatic arthritis].", "content": "1. Penicyllamin is a useful drug in the treatment of some cases of severe rheumatoid arthritis, or in unsuccessful therapeutics. 2. It can lead to remission or to antiinflamatory activity from 20 to 40%. 3. It has a high percentage of iatrogenic effects, but they are generally slight and in a minor percentage the alterations are severe (hematological mainly). 4. Its use requires: a) Correct prescription. b) Careful clinic and paraclinic observation. c) Experience and knowledge in rheumatologic speciality. 5. It may be of use in other connective tissue diseases or in immunological processes with disfavorable consequences to the organism.", "contents": "[Therapeutic efficiency of penicillamine D in rheumatic arthritis]. 1. Penicyllamin is a useful drug in the treatment of some cases of severe rheumatoid arthritis, or in unsuccessful therapeutics. 2. It can lead to remission or to antiinflamatory activity from 20 to 40%. 3. It has a high percentage of iatrogenic effects, but they are generally slight and in a minor percentage the alterations are severe (hematological mainly). 4. Its use requires: a) Correct prescription. b) Careful clinic and paraclinic observation. c) Experience and knowledge in rheumatologic speciality. 5. It may be of use in other connective tissue diseases or in immunological processes with disfavorable consequences to the organism."}
{"id": "PMID:485673", "title": "[Jugular phlebogram after cardiac surgery].", "content": "We studied several parameters of the jugular flebogram in 70 patients who had undergone cardiac surgery. Fifty six of them were on sinusal rithm and 14 in auricular fibrillation. We found among them 39 who had undergone commissurotomy and 16 who had got valvular prothesis. The results of these studies were analyzed and compared with those of 94 normal subjects, taking in consideration the type of surgical intervention and the time elapsed since the operation was performed--the latter in the more numerous group. It is interesting to observe that the sinus grows deeper after the intervention so making the relation xv/yv lower than the unit in 33% of the patients maintaining sinusal rithm. This alteration remained stationary during some years in some of the cases, the results suggesting a greater frequency during the first months of the post-operatory period. We didn't find any relation of this morphologic alteration with the presence of a pericardial construction, pulmonary hypertension, valvular injuries with repercussion on the right heart or modifications on the nenous pressure which we think due to functional changes following the pericardiotomy. It is interesting to observe these mechanographic signs in relation with the post-operatory period of cardiac surgery, but we must avoid hypervaluation of those cases suspicious of pericardial constriction.", "contents": "[Jugular phlebogram after cardiac surgery]. We studied several parameters of the jugular flebogram in 70 patients who had undergone cardiac surgery. Fifty six of them were on sinusal rithm and 14 in auricular fibrillation. We found among them 39 who had undergone commissurotomy and 16 who had got valvular prothesis. The results of these studies were analyzed and compared with those of 94 normal subjects, taking in consideration the type of surgical intervention and the time elapsed since the operation was performed--the latter in the more numerous group. It is interesting to observe that the sinus grows deeper after the intervention so making the relation xv/yv lower than the unit in 33% of the patients maintaining sinusal rithm. This alteration remained stationary during some years in some of the cases, the results suggesting a greater frequency during the first months of the post-operatory period. We didn't find any relation of this morphologic alteration with the presence of a pericardial construction, pulmonary hypertension, valvular injuries with repercussion on the right heart or modifications on the nenous pressure which we think due to functional changes following the pericardiotomy. It is interesting to observe these mechanographic signs in relation with the post-operatory period of cardiac surgery, but we must avoid hypervaluation of those cases suspicious of pericardial constriction."}
{"id": "PMID:485680", "title": "Canine gastrointestinal motility effects of prostaglandin F2 alpha in vivo.", "content": "Prostaglandin F2 alpha (PGF2 alpha) has recently been implicated in the pathogenesis of some diarrhea in man. PGF2 alpha has been shown to increase the smooth muscle contractile motility of some gastrointestinal muscles in vitro. The in vivo effects of PGF2 alpha on bowel smooth muscles are not clearly delineated. The aim of this study was to investigate the effect of PGF2 alpha on motility of the small and large intestine in the anesthetized dog. Six contractile force transducers were implanted to record contractions from both the circular and longitudinal muscles of the duodenum, ileum and colon. Blood pressure was monitored from the femoral artery and drug injections were made in the femoral vein. The i.v. administration of PGF2 alpha (1 microgram/kg/min) significantly stimulated duodenal circular muscle contractile frequency while depressing the longitudinal muscle contractile tone. In the ileum, PGF2 alpha markedly stimulated circular and longitudinal muscle contractions. In the circular ileum, tone was significantly increased while in the longitudinal ileum it was significantly decreased. In the colon PGF2 alpha did not significantly affect intestinal motility. These results suggest that the diarrheal effects of PGF2 alpha may be related to an effect on small bowel rather than on large bowel motility in the dog.", "contents": "Canine gastrointestinal motility effects of prostaglandin F2 alpha in vivo. Prostaglandin F2 alpha (PGF2 alpha) has recently been implicated in the pathogenesis of some diarrhea in man. PGF2 alpha has been shown to increase the smooth muscle contractile motility of some gastrointestinal muscles in vitro. The in vivo effects of PGF2 alpha on bowel smooth muscles are not clearly delineated. The aim of this study was to investigate the effect of PGF2 alpha on motility of the small and large intestine in the anesthetized dog. Six contractile force transducers were implanted to record contractions from both the circular and longitudinal muscles of the duodenum, ileum and colon. Blood pressure was monitored from the femoral artery and drug injections were made in the femoral vein. The i.v. administration of PGF2 alpha (1 microgram/kg/min) significantly stimulated duodenal circular muscle contractile frequency while depressing the longitudinal muscle contractile tone. In the ileum, PGF2 alpha markedly stimulated circular and longitudinal muscle contractions. In the circular ileum, tone was significantly increased while in the longitudinal ileum it was significantly decreased. In the colon PGF2 alpha did not significantly affect intestinal motility. These results suggest that the diarrheal effects of PGF2 alpha may be related to an effect on small bowel rather than on large bowel motility in the dog."}
{"id": "PMID:485672", "title": "[Management of persistent ductus arteriosus in the newborn with respiratory insufficiency].", "content": "Sixteen newborn babies with severe respiratory distress and patent ductus arteriosus with congestive heart failure are presented. Fifteen of them were premature and one at full term. Management consisted of the accepted medical and ventilatory aids such as digitalis, diuretics, ventilators, etc. Thirteen cases were given indomethacin with clinical closure in five of them (38%). Ten cases underwent surgical closure with 30% mortality. It is recommended that these patients be given indomethacin inicially; if there is no response in 36 hours surgical closure of the duct should be performed prior to progressive deterioration and eventual death.", "contents": "[Management of persistent ductus arteriosus in the newborn with respiratory insufficiency]. Sixteen newborn babies with severe respiratory distress and patent ductus arteriosus with congestive heart failure are presented. Fifteen of them were premature and one at full term. Management consisted of the accepted medical and ventilatory aids such as digitalis, diuretics, ventilators, etc. Thirteen cases were given indomethacin with clinical closure in five of them (38%). Ten cases underwent surgical closure with 30% mortality. It is recommended that these patients be given indomethacin inicially; if there is no response in 36 hours surgical closure of the duct should be performed prior to progressive deterioration and eventual death."}
{"id": "PMID:485681", "title": "Studies on the effects of anti-inflammatory action of benzoyl-hydrotropic acid (ketoprofen) and other drugs, with special reference to prostaglandin synthesis.", "content": "Effects of ketoprofen, indomethacin, phenylbutazone and acetylsalicylic acid on edema and on in vivo and in vitro synthesis of PGE1, PGE2 and PGF2 alpha in response to carrageenin were studied in rats. Ketoprofen was a potent drug in depressing edema and increased PGE1, PGE2 and PGF2 alpha in response to carrageenin. Ketoprofen also inhibited in vitro synthesis of PGE2 and PGF2 alpha from arachidonic acid. The relative potency of ketoprofen in those parameters was comparable to or greater than that of indomethacin. Phenylbutazone and acetyl-salicylic acid were, however, less potent in this respect. It is suggested that ketoprofen manifests its anti-inflammatory action, at least in part, by inhibiting prostaglandin synthesis.", "contents": "Studies on the effects of anti-inflammatory action of benzoyl-hydrotropic acid (ketoprofen) and other drugs, with special reference to prostaglandin synthesis. Effects of ketoprofen, indomethacin, phenylbutazone and acetylsalicylic acid on edema and on in vivo and in vitro synthesis of PGE1, PGE2 and PGF2 alpha in response to carrageenin were studied in rats. Ketoprofen was a potent drug in depressing edema and increased PGE1, PGE2 and PGF2 alpha in response to carrageenin. Ketoprofen also inhibited in vitro synthesis of PGE2 and PGF2 alpha from arachidonic acid. The relative potency of ketoprofen in those parameters was comparable to or greater than that of indomethacin. Phenylbutazone and acetyl-salicylic acid were, however, less potent in this respect. It is suggested that ketoprofen manifests its anti-inflammatory action, at least in part, by inhibiting prostaglandin synthesis."}
{"id": "PMID:485682", "title": "Developmental change in striatal concentration of homovanillic acid and 3,4-dihydroxyphenylacetic acid in response to apomorphine and haloperidol treatment.", "content": "The striatal concentration of homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) was estimated following an injection of apomorphine into the developing rat subchronically treated (S.C.) with haloperidol. At the withdrawal stage of haloperidol treatment, striatal HVA and DOPAC levels decreased in the rat aged 14, 24 and 34 days. The haloperidol treatment enhanced the apomorphine-induced reduction of the striatal HVA content at the withdrawal stage in the 24-day-old and 34-day-old rat. These results suggest that a functional link in the feedback control mechanism and dopamine receptors do not fully develop at birth but do so in the early stage of postnatal life.", "contents": "Developmental change in striatal concentration of homovanillic acid and 3,4-dihydroxyphenylacetic acid in response to apomorphine and haloperidol treatment. The striatal concentration of homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) was estimated following an injection of apomorphine into the developing rat subchronically treated (S.C.) with haloperidol. At the withdrawal stage of haloperidol treatment, striatal HVA and DOPAC levels decreased in the rat aged 14, 24 and 34 days. The haloperidol treatment enhanced the apomorphine-induced reduction of the striatal HVA content at the withdrawal stage in the 24-day-old and 34-day-old rat. These results suggest that a functional link in the feedback control mechanism and dopamine receptors do not fully develop at birth but do so in the early stage of postnatal life."}
{"id": "PMID:485683", "title": "Effect of morphine on the clearance of endogenous creatinine and blood clinical chemicals in the rat.", "content": "The effect of morphine on urine and blood serum concentration of Na+, K+, Cl- and other chemicals was determined using Sprague Dawley rats, previously hydrated with 50 ml/kg of 0.5% NaCl to provoke an increased diuresis. Morphine caused about a 90% decrease in the urine concentration of Na+, K+ and Cl-. This reduction in the urine electrolyte excretion was associated with a small but significant increase in the concentration of the blood Na+ and Cl-. The antidiuresis and the decrease in urine electrolytes caused by morphine was paralleled by a 50% reduction of the endogenous creatinine clearance. An acute dose of 7.5 mg/kg morphine i.p. did not alter the concentration of 13 other clinical blood chemicals with the exception of urea. However, after 18 hr of morphine pellet implantation, a significant increase was found in the blood urea and in the activity of serum transaminases. Lactic dehydrogenases isoenzymes one and two were significantly increased in the blood of rats chronically administered with morphine.", "contents": "Effect of morphine on the clearance of endogenous creatinine and blood clinical chemicals in the rat. The effect of morphine on urine and blood serum concentration of Na+, K+, Cl- and other chemicals was determined using Sprague Dawley rats, previously hydrated with 50 ml/kg of 0.5% NaCl to provoke an increased diuresis. Morphine caused about a 90% decrease in the urine concentration of Na+, K+ and Cl-. This reduction in the urine electrolyte excretion was associated with a small but significant increase in the concentration of the blood Na+ and Cl-. The antidiuresis and the decrease in urine electrolytes caused by morphine was paralleled by a 50% reduction of the endogenous creatinine clearance. An acute dose of 7.5 mg/kg morphine i.p. did not alter the concentration of 13 other clinical blood chemicals with the exception of urea. However, after 18 hr of morphine pellet implantation, a significant increase was found in the blood urea and in the activity of serum transaminases. Lactic dehydrogenases isoenzymes one and two were significantly increased in the blood of rats chronically administered with morphine."}
{"id": "PMID:485684", "title": "Influence of dimethyl sulfoxide (Me2SO) on vascular smooth muscle.", "content": "The present investigation was undertaken to determine the action of dimethyl sulfoxide (Me2SO) on vascular smooth muscle. The rabbit isolated aortic strip preparation was used. In the present study, Me2SO-induced relaxation of isolated rabbit aorta was demonstrated. The degree of relaxation induced by Me2SO was concentration dependent. In the presence of Me2SO the action of norepinephrine, histamine, carbachol and potassium was significantly (P less than .05) antagonized. The control ED50 values were 5.36 + 0.50 + 10(-8) M, 3.38 +/- 0.74 X 10(-6) M, 1.40 +/- 0.82 X 10(-6) M and 2.43 +/- 0.19 x 10(-2) M, respectively. In the presence of Me2SO the ED50 values were 2.23 +/- 0.23 x 10(-6) M, 1.80 +/- 0.40 X 10(-5) M, 1.00 +/- 0.38 X 10(-4) and 4.21 +/- 0.07 x 10(-2) M, respectively. The maximum response of the tissue to the various vasoactive agents was significantly depressed in the presence of Me2SO. When Me2SO was present in the efflux medium, the amount of 45Ca2+ washed out of aortic strips was significantly (P less than .05) reduced when compared to control. The 45Ca2+ efflux studies supported the hypothesis that Me2SO-induced relaxation could possibly result from enhanced binding of activator Ca2+ to high affinity Ca2+ sites.", "contents": "Influence of dimethyl sulfoxide (Me2SO) on vascular smooth muscle. The present investigation was undertaken to determine the action of dimethyl sulfoxide (Me2SO) on vascular smooth muscle. The rabbit isolated aortic strip preparation was used. In the present study, Me2SO-induced relaxation of isolated rabbit aorta was demonstrated. The degree of relaxation induced by Me2SO was concentration dependent. In the presence of Me2SO the action of norepinephrine, histamine, carbachol and potassium was significantly (P less than .05) antagonized. The control ED50 values were 5.36 + 0.50 + 10(-8) M, 3.38 +/- 0.74 X 10(-6) M, 1.40 +/- 0.82 X 10(-6) M and 2.43 +/- 0.19 x 10(-2) M, respectively. In the presence of Me2SO the ED50 values were 2.23 +/- 0.23 x 10(-6) M, 1.80 +/- 0.40 X 10(-5) M, 1.00 +/- 0.38 X 10(-4) and 4.21 +/- 0.07 x 10(-2) M, respectively. The maximum response of the tissue to the various vasoactive agents was significantly depressed in the presence of Me2SO. When Me2SO was present in the efflux medium, the amount of 45Ca2+ washed out of aortic strips was significantly (P less than .05) reduced when compared to control. The 45Ca2+ efflux studies supported the hypothesis that Me2SO-induced relaxation could possibly result from enhanced binding of activator Ca2+ to high affinity Ca2+ sites."}
{"id": "PMID:485675", "title": "[Paradox arterial embolism: diagnosis during life].", "content": "Paradoxical systemic embolism in the absence of congenital heart disease is often considered a clinical or pathologic rarity. We have recently observed two cases of paradoxical systemic emboli, secondary to massive pulmonary embolization in the presence of a patient foramen ovale in an otherwise normal heart. One case was lethal and the diagnosis was confirmed at autopsy. The other case was diagnosed by cardiac catheterization with dye dilution curves and the patient recovered after recurrences were prevented by the placement of an inferior vena cava umbrella-filter. Paradoxical embolization should be suspected when systemic emboli occur without an apparent cause. The diagnosis can be established by cardiac catheterization, pulmonary angiography and phlebography, and recurrences can be prevented by anticoagulation and partial interruption of the inferior vena cava, when the emboli arise from its territory.", "contents": "[Paradox arterial embolism: diagnosis during life]. Paradoxical systemic embolism in the absence of congenital heart disease is often considered a clinical or pathologic rarity. We have recently observed two cases of paradoxical systemic emboli, secondary to massive pulmonary embolization in the presence of a patient foramen ovale in an otherwise normal heart. One case was lethal and the diagnosis was confirmed at autopsy. The other case was diagnosed by cardiac catheterization with dye dilution curves and the patient recovered after recurrences were prevented by the placement of an inferior vena cava umbrella-filter. Paradoxical embolization should be suspected when systemic emboli occur without an apparent cause. The diagnosis can be established by cardiac catheterization, pulmonary angiography and phlebography, and recurrences can be prevented by anticoagulation and partial interruption of the inferior vena cava, when the emboli arise from its territory."}
{"id": "PMID:485685", "title": "Autoradiographic evidence for passage of vincamine through the blood-brain barrier.", "content": "Autoradiographies of the brain and of the cerebellum were performed in 8-month old rats intraperitoneal injection of 3H-labelled vincamine. Only those samples that were processed by freeze-drying or by freeze-substitution gave interpretable results. Some specifically labeled cells were found in the molecular layer of the cerebellar cortex as well as in the internal granular and in the deeper layers of the cerebral cortex. They did not belong to the vascular system. It was concluded that vincamine or its metabolites crosses the blood-brain barrier. Any attempt to explain a possible action of the substance on the brain function should thus also consider the cellular components of the central nervous system and not only the haemodynamics of the cerebral circulation.", "contents": "Autoradiographic evidence for passage of vincamine through the blood-brain barrier. Autoradiographies of the brain and of the cerebellum were performed in 8-month old rats intraperitoneal injection of 3H-labelled vincamine. Only those samples that were processed by freeze-drying or by freeze-substitution gave interpretable results. Some specifically labeled cells were found in the molecular layer of the cerebellar cortex as well as in the internal granular and in the deeper layers of the cerebral cortex. They did not belong to the vascular system. It was concluded that vincamine or its metabolites crosses the blood-brain barrier. Any attempt to explain a possible action of the substance on the brain function should thus also consider the cellular components of the central nervous system and not only the haemodynamics of the cerebral circulation."}
{"id": "PMID:485686", "title": "The effects of long-term oral treatment with chlorothiazide or furosemide on hereditary diabetes insipidus in rats.", "content": "The polyuria of homozygous Brattleboro (BB) female rats is halved when they are given chlorothiazide (about 250 mg/day) or furosemide (about 60 mg/day) orally for one day. The effect of chlorothiazide is still found after 16 days of treatment, whereas the effect of furosemide entirely disappears within 5 days. Both diuretics induce chronically increased plasma renin activity (PRA) and decreased natriuresis as long as they are added to the diet; the effect on Na is more evident during furosemide treatment. Urinary urea content as well as urinary osmolality are increased by chlorothiazide and \"free water\" output is normalized. Furosemide does not affect urea content and decreases urinary osmolality from the start, as compared to untreated BB homozygotes; it raises \"free water\" output above BB values.", "contents": "The effects of long-term oral treatment with chlorothiazide or furosemide on hereditary diabetes insipidus in rats. The polyuria of homozygous Brattleboro (BB) female rats is halved when they are given chlorothiazide (about 250 mg/day) or furosemide (about 60 mg/day) orally for one day. The effect of chlorothiazide is still found after 16 days of treatment, whereas the effect of furosemide entirely disappears within 5 days. Both diuretics induce chronically increased plasma renin activity (PRA) and decreased natriuresis as long as they are added to the diet; the effect on Na is more evident during furosemide treatment. Urinary urea content as well as urinary osmolality are increased by chlorothiazide and \"free water\" output is normalized. Furosemide does not affect urea content and decreases urinary osmolality from the start, as compared to untreated BB homozygotes; it raises \"free water\" output above BB values."}
{"id": "PMID:485687", "title": "The influence of 2,2-dimethyl-1-(4-methylphenyl)-1-propanone (SaH 50-283) on food efficiency in rats.", "content": "The present studies were undertaken to investigate the possible mechanism(s) of action of 2,2-dimethyl-1-(4-methylphenyl)-1-propanone (SaH 50-283) on food efficiency in rats. SaH 50-283, unlike phenformin (DBI), did not inhibit glucose absorption. However, hyperglycemia induced by oral maltose, lactose or starch load was markedly inhibited in animals pretreated with SaH 50-283. The ED25 for lowering blood sugar levels following an oral maltose load was calculated to be 12 mg/kg. SaH 50-283 could be administered as long as 7 hr prior to a maltose load and still maintain its effect. Food efficiency was significantly (P less than .01) lowered in rats pretreated with SaH 50-283 1 hr prior to a 2 hr feeding period of a purified high carbohydrate diet. It was concluded that the lowering of maltase activity in the brush border of animals treated with SaH 50-283 could partially account for its mechanism of action in lowering food efficiency in rats.", "contents": "The influence of 2,2-dimethyl-1-(4-methylphenyl)-1-propanone (SaH 50-283) on food efficiency in rats. The present studies were undertaken to investigate the possible mechanism(s) of action of 2,2-dimethyl-1-(4-methylphenyl)-1-propanone (SaH 50-283) on food efficiency in rats. SaH 50-283, unlike phenformin (DBI), did not inhibit glucose absorption. However, hyperglycemia induced by oral maltose, lactose or starch load was markedly inhibited in animals pretreated with SaH 50-283. The ED25 for lowering blood sugar levels following an oral maltose load was calculated to be 12 mg/kg. SaH 50-283 could be administered as long as 7 hr prior to a maltose load and still maintain its effect. Food efficiency was significantly (P less than .01) lowered in rats pretreated with SaH 50-283 1 hr prior to a 2 hr feeding period of a purified high carbohydrate diet. It was concluded that the lowering of maltase activity in the brush border of animals treated with SaH 50-283 could partially account for its mechanism of action in lowering food efficiency in rats."}
{"id": "PMID:485688", "title": "Central nervous system responses to cigarette smoke inhalation in the cat.", "content": "Intact, pentobarbital anesthetized cats (with and without brainstem stimulating electrode implants) and unanesthetized Sherrington (gamma-driven) decerebrate cats \"smoked\" cigarettes of varying nicotine content (0.2-2.5 mg). Nicotine free lettuce leaf cigarettes were used as controls. \"Smoking doses\" of nicotine base (10-25 micrograms/kg) were administered i.v. for comparison. Smoke inhalation produced motor reflex depression which paralleled the nicotine content of the cigarettes \"smoked\". Patellar reflex facilitation due to mesencephalic reticular stimulation was reduced by doses of nicotine and cigarette smoke. Cigarettes (2.5 mg nicotine) and doses of nicotine (25-50 micrograms/kg, i.v.) significantly reduced rigidity and patellar reflex amplitude in the gamma-decerebrate cat. Dihydro-beta-erythroidine reduced the nicotine and cigarette smoke induced patellar reflex depression but not the diminution in the rigidity. Smoking doses of nicotine suppressed pentobarbital-induced EEG spindles in acutely prepared cats. Nicotine (10-25 micrograms/kg) produced EEG and behavioral arousal in cats with chronic deep electrodes. It was concluded that cigarette smoke produces its pharmacological effects via its nicotine content.", "contents": "Central nervous system responses to cigarette smoke inhalation in the cat. Intact, pentobarbital anesthetized cats (with and without brainstem stimulating electrode implants) and unanesthetized Sherrington (gamma-driven) decerebrate cats \"smoked\" cigarettes of varying nicotine content (0.2-2.5 mg). Nicotine free lettuce leaf cigarettes were used as controls. \"Smoking doses\" of nicotine base (10-25 micrograms/kg) were administered i.v. for comparison. Smoke inhalation produced motor reflex depression which paralleled the nicotine content of the cigarettes \"smoked\". Patellar reflex facilitation due to mesencephalic reticular stimulation was reduced by doses of nicotine and cigarette smoke. Cigarettes (2.5 mg nicotine) and doses of nicotine (25-50 micrograms/kg, i.v.) significantly reduced rigidity and patellar reflex amplitude in the gamma-decerebrate cat. Dihydro-beta-erythroidine reduced the nicotine and cigarette smoke induced patellar reflex depression but not the diminution in the rigidity. Smoking doses of nicotine suppressed pentobarbital-induced EEG spindles in acutely prepared cats. Nicotine (10-25 micrograms/kg) produced EEG and behavioral arousal in cats with chronic deep electrodes. It was concluded that cigarette smoke produces its pharmacological effects via its nicotine content."}
{"id": "PMID:485689", "title": "Evidence for a noncholinergic site for nicotine's action in brain: psychopharmacological, electrophysiological and receptor binding studies.", "content": "In an effort to investigate the possibility of noncholinergic nicotine sites within the brain, psychopharmacological, biochemical and eletrophysiological studies were undertaken with nicotine and various newly synthesized derivatives of nicotine and piperidine. When 1-10 micrograms of (-)-nicotine was injected into the region of the lateral ventricle of rats through implanted cannulae, there resulted a characteristic prostration immobilization syndrome, which was accompanied by seizures and tremors at the higher dose range. The (+)-isomer possessed 1/100 the activity of the natural (-)-isomer. The syndrome could be prevented by pre-treatment, intraventricularly, with the N-benzyl and N-p-nitrophenylazido derivates of either nicotine or piperide. A variety of neurotransmitters and psychotropic agents, including acetylcholine and anticholinergic drugs, were without antagonistic action. After nicotine, recordings of spontaneous electrical activity from electrodes chronically implanted into the region of the dorsal hippocampus showed a marked decrease in the amplitude and number of 6-8 sec discharges, and the change was correlated with the behavioral syndrome. Receptor binding studies were performed with rat brain slices and various neural preparations using 3H-nicotine, 125I-alpha-bungarotoxin and 14C-d-tubocurarine as ligands; and only with 3H-nicotine was it possible to demostrate any competitive effect with the various nicotine and piperidine antagonists. It was possible to demonstrate stereospecific or specific nicotine binding to only glass fiber filters and, to a lesser extent, brain slices, but not to cell-free preparations. It was concluded that there existed specific noncholinergic sites for nicotine's action which have not been hitherto described.", "contents": "Evidence for a noncholinergic site for nicotine's action in brain: psychopharmacological, electrophysiological and receptor binding studies. In an effort to investigate the possibility of noncholinergic nicotine sites within the brain, psychopharmacological, biochemical and eletrophysiological studies were undertaken with nicotine and various newly synthesized derivatives of nicotine and piperidine. When 1-10 micrograms of (-)-nicotine was injected into the region of the lateral ventricle of rats through implanted cannulae, there resulted a characteristic prostration immobilization syndrome, which was accompanied by seizures and tremors at the higher dose range. The (+)-isomer possessed 1/100 the activity of the natural (-)-isomer. The syndrome could be prevented by pre-treatment, intraventricularly, with the N-benzyl and N-p-nitrophenylazido derivates of either nicotine or piperide. A variety of neurotransmitters and psychotropic agents, including acetylcholine and anticholinergic drugs, were without antagonistic action. After nicotine, recordings of spontaneous electrical activity from electrodes chronically implanted into the region of the dorsal hippocampus showed a marked decrease in the amplitude and number of 6-8 sec discharges, and the change was correlated with the behavioral syndrome. Receptor binding studies were performed with rat brain slices and various neural preparations using 3H-nicotine, 125I-alpha-bungarotoxin and 14C-d-tubocurarine as ligands; and only with 3H-nicotine was it possible to demostrate any competitive effect with the various nicotine and piperidine antagonists. It was possible to demonstrate stereospecific or specific nicotine binding to only glass fiber filters and, to a lesser extent, brain slices, but not to cell-free preparations. It was concluded that there existed specific noncholinergic sites for nicotine's action which have not been hitherto described."}
{"id": "PMID:485690", "title": "Morphine and naloxone effects on olfactory evoked electrographic activity in the amygdala.", "content": "This research tested morphine and naloxone effects on evoked EEG and unit activity in 3 opiate-relevant brain areas in response to electrical stimulation of the olfactory bulb in acute, unanesthesized rat preparations. Stimulation evoked clear EEG responses in the amygdala (Amyg) and sometimes in the other areas (caudate and central grey); morphine (15 mg/kg) depressed the Amyg response in some rats, but enhanced it in others, and naloxone usually reversed both kinds of effect. Stimulation caused excitatory unit impulse reponses in the Amyg, and morphine unexpectedly increased the magnitude of the stimulus-evoked excitation; naloxone reversed this enhancement. In control rats, naloxone often decreased the Amyg evoked response. Stimulus-evoked increases in unit activity in the caudate and central grey, when they did occur, were depressed by morphine, but naxoxone had no consistent reversing effect. Both the EEG and unit data indicate that morphine excites, or disinhibits, certain neurons associated with the olfactory-Amyg pathway. There was also some evidence that this pathway contains endorphinergic elements.", "contents": "Morphine and naloxone effects on olfactory evoked electrographic activity in the amygdala. This research tested morphine and naloxone effects on evoked EEG and unit activity in 3 opiate-relevant brain areas in response to electrical stimulation of the olfactory bulb in acute, unanesthesized rat preparations. Stimulation evoked clear EEG responses in the amygdala (Amyg) and sometimes in the other areas (caudate and central grey); morphine (15 mg/kg) depressed the Amyg response in some rats, but enhanced it in others, and naloxone usually reversed both kinds of effect. Stimulation caused excitatory unit impulse reponses in the Amyg, and morphine unexpectedly increased the magnitude of the stimulus-evoked excitation; naloxone reversed this enhancement. In control rats, naloxone often decreased the Amyg evoked response. Stimulus-evoked increases in unit activity in the caudate and central grey, when they did occur, were depressed by morphine, but naxoxone had no consistent reversing effect. Both the EEG and unit data indicate that morphine excites, or disinhibits, certain neurons associated with the olfactory-Amyg pathway. There was also some evidence that this pathway contains endorphinergic elements."}
{"id": "PMID:485691", "title": "Anticonvulsant action in a series of benzimidazole anthelmintics.", "content": "In mice, the electroconvulsant threshold was elevated after oral administration of 7 benzimidazole anthelmintics at a dose of 100 mg/kg. This effect was most pronounced with cambendazole which also showed an outstanding duration of action. None of the drugs tested elevated the pentetrazole seizure threshold.", "contents": "Anticonvulsant action in a series of benzimidazole anthelmintics. In mice, the electroconvulsant threshold was elevated after oral administration of 7 benzimidazole anthelmintics at a dose of 100 mg/kg. This effect was most pronounced with cambendazole which also showed an outstanding duration of action. None of the drugs tested elevated the pentetrazole seizure threshold."}
{"id": "PMID:485692", "title": "The effect of local anaesthetic agents on afferent and motor nerve impulses in the frog.", "content": "In the frog nerve-muscle preparation an attempt was made to determine the sites of local anaesthetic activity. The drugs were applied not only to the nerve fiber, but also to other structures of the nerve. Different blocking concentrations proved that the drugs effected different sites. It was shown that small concentrations of the agents effected the sensory endings of afferent nerves and the muscle spindles. The muscle spindle needed the smallest minimal concentration (Cm) to be blocked. The sensory endings of the C fibers needed a higher one and the motor terminal a still higher one. However, all these concentrations belonged to a similar range (3 X, 4 X and 5 X 10(-5) g/ml bupivacaine or etidocaine). To block the nerve trunk a much higher Cm was mandatory (4 X 10(-4) for C fibers and greater than 5 X 10(-4) g/ml for A alpha fibers). This means that the effect of the local anaesthetic drugs on the nervous system is not single but multiple.", "contents": "The effect of local anaesthetic agents on afferent and motor nerve impulses in the frog. In the frog nerve-muscle preparation an attempt was made to determine the sites of local anaesthetic activity. The drugs were applied not only to the nerve fiber, but also to other structures of the nerve. Different blocking concentrations proved that the drugs effected different sites. It was shown that small concentrations of the agents effected the sensory endings of afferent nerves and the muscle spindles. The muscle spindle needed the smallest minimal concentration (Cm) to be blocked. The sensory endings of the C fibers needed a higher one and the motor terminal a still higher one. However, all these concentrations belonged to a similar range (3 X, 4 X and 5 X 10(-5) g/ml bupivacaine or etidocaine). To block the nerve trunk a much higher Cm was mandatory (4 X 10(-4) for C fibers and greater than 5 X 10(-4) g/ml for A alpha fibers). This means that the effect of the local anaesthetic drugs on the nervous system is not single but multiple."}
{"id": "PMID:485693", "title": "Simultaneous assessment of the effects of trimetazidine on the myocardium and coronary vasculature of the dog.", "content": "The effects of trimetazidine on the electrical and mechanical activities of the myocardium were investigated in comparison with the effect on the coronary blood flow in blood-perfused preparations excised from dog hearts. In the sinoatrial (SA) node preparation trimetazidine injected into the sinus node artery increased blood flow and depressed SA node automaticity. In the atrioventricular (AV) node preparation trimetazidine injected into the AV node artery or the anterior septal artery increased blood flow and equally prolonged AV conduction time. In the papillary muscle preparation trimetazidine injected into the anterior septal artery increased blood flow and depressed force of contraction. These cardiodepressant actions occurred in the same range of doses of trimetazidine that exerted the vasodilator action. This indicates that trimetazidine has no selective activity on the coronary vasculature alone. Moreover, the vasodilator effect was more short-lived than the cardiodepressant action. Trimetazidine injected into the anterior septal artery affected ventricular bipolar electrograms. This, taken together with the prolongation of AV conduction time obtained with the same route of administration, suggests that the cardiodepressant action of trimetazidine is ascribed to the local anaesthetic or the membrane stabilizing action rather than to the calcium-antagonistic action.", "contents": "Simultaneous assessment of the effects of trimetazidine on the myocardium and coronary vasculature of the dog. The effects of trimetazidine on the electrical and mechanical activities of the myocardium were investigated in comparison with the effect on the coronary blood flow in blood-perfused preparations excised from dog hearts. In the sinoatrial (SA) node preparation trimetazidine injected into the sinus node artery increased blood flow and depressed SA node automaticity. In the atrioventricular (AV) node preparation trimetazidine injected into the AV node artery or the anterior septal artery increased blood flow and equally prolonged AV conduction time. In the papillary muscle preparation trimetazidine injected into the anterior septal artery increased blood flow and depressed force of contraction. These cardiodepressant actions occurred in the same range of doses of trimetazidine that exerted the vasodilator action. This indicates that trimetazidine has no selective activity on the coronary vasculature alone. Moreover, the vasodilator effect was more short-lived than the cardiodepressant action. Trimetazidine injected into the anterior septal artery affected ventricular bipolar electrograms. This, taken together with the prolongation of AV conduction time obtained with the same route of administration, suggests that the cardiodepressant action of trimetazidine is ascribed to the local anaesthetic or the membrane stabilizing action rather than to the calcium-antagonistic action."}
{"id": "PMID:485694", "title": "Myocardial biochemical modifications induced by theophylline with reference to its value as antianginal drug.", "content": "Theophylline-induced variations of cardiac metabolism have been investigated by determining concentrations of various energetic substrates and of high-energy phosphates in myocardial tissue, the repeated sampling of myocardium being made possible by an extracorporal circulation system. When administered in therapeutic, or even slightly higher doses, theophylline does not modify triglyceride, glycerol and free fatty acid content or phosphocreatine and ATP content in subepicardial and subendocardial layers, but it does lower glycogen and raise lactate concentration. Consequently, the changes in anaerobic glycolysis due to myocardial ischemia may be enhanced if, as is probably the case, theophylline fails to restore the supply of oxygen.", "contents": "Myocardial biochemical modifications induced by theophylline with reference to its value as antianginal drug. Theophylline-induced variations of cardiac metabolism have been investigated by determining concentrations of various energetic substrates and of high-energy phosphates in myocardial tissue, the repeated sampling of myocardium being made possible by an extracorporal circulation system. When administered in therapeutic, or even slightly higher doses, theophylline does not modify triglyceride, glycerol and free fatty acid content or phosphocreatine and ATP content in subepicardial and subendocardial layers, but it does lower glycogen and raise lactate concentration. Consequently, the changes in anaerobic glycolysis due to myocardial ischemia may be enhanced if, as is probably the case, theophylline fails to restore the supply of oxygen."}
{"id": "PMID:485695", "title": "Relative vasodilator potency of ATP, ADP, AMP and adenosine (Ado) in the cat hind limb.", "content": "The relative vasodilator potencies of ATP, ADP, AMP and Ado were determined by bioassay using the skinned and unskinned cat hind limb and comparing the increase in blood flow induced by intra-arterial injection of the adenyl compounds. The relative vasodilator potencies of these agents were similar in either hind limb preparation and were as follows: ADP greater than ATP greater than Ado greater than or equal to AMP. ADP was 2-4 times more potent than ATP and about 200 times more potent than Ado or AMP. When the adenyl compounds were incubated in blood for 2 min before injection, their potencies were not changed in the bioassay. Furthermore, in a chemical assay, after Ado and ADP were incubated for 2 min in cat blood (at the concentrations used in the bioassay) only 3% of Ado and 29% of ADP were metabolized. The results indicate that ATP and ADP exert a vasodilator effect that is not dependent upon their conversion to Ado. This deduction cannot be made for AMP because its relative potency was quite similar to Ado. Since a similar relative potency was found in the skinned and the unskinned hind limb, it is concluded that the vasodilator effect due to ATP and ADP is occurring in skeletal muscle but this does not exclude simultaneous cutaneous vasodilation. The vasodilator effect of adenyl compounds may be related to purinergic receptor activation.", "contents": "Relative vasodilator potency of ATP, ADP, AMP and adenosine (Ado) in the cat hind limb. The relative vasodilator potencies of ATP, ADP, AMP and Ado were determined by bioassay using the skinned and unskinned cat hind limb and comparing the increase in blood flow induced by intra-arterial injection of the adenyl compounds. The relative vasodilator potencies of these agents were similar in either hind limb preparation and were as follows: ADP greater than ATP greater than Ado greater than or equal to AMP. ADP was 2-4 times more potent than ATP and about 200 times more potent than Ado or AMP. When the adenyl compounds were incubated in blood for 2 min before injection, their potencies were not changed in the bioassay. Furthermore, in a chemical assay, after Ado and ADP were incubated for 2 min in cat blood (at the concentrations used in the bioassay) only 3% of Ado and 29% of ADP were metabolized. The results indicate that ATP and ADP exert a vasodilator effect that is not dependent upon their conversion to Ado. This deduction cannot be made for AMP because its relative potency was quite similar to Ado. Since a similar relative potency was found in the skinned and the unskinned hind limb, it is concluded that the vasodilator effect due to ATP and ADP is occurring in skeletal muscle but this does not exclude simultaneous cutaneous vasodilation. The vasodilator effect of adenyl compounds may be related to purinergic receptor activation."}
{"id": "PMID:485696", "title": "Effect of sodium taurocholate on the hepatic transport of bromsulphthalein in rats.", "content": "In anaesthetized, bile duct-cannulated rats the hepatic transport of bromsulphthalein (BSP) showed a dose-dependent increase in response to simultaneous administration of taurocholate (TC). The excretion rates of both free (BSP) and conjugated bromsulphthalein (BSP-GSH) were significantly elevated by TC. The simultaneously given TC decreased the hepatic uptake of BSP and BSP-GSH, but did not influence the conjugation of BSP with glutathione (GSH) or the biliary excretion rate of exogenous BSP-GSH. When the liver was depleted of GSH by pretreatment with diethyl-maleate (DEM), TC increased the excretion of free BSP. In DEM-treated rats free BSP markedly depressed the biliary excretion of exogenous BSP-GSH. When TC was given simultaneously with BSP, the inhibitory effect of BSP on the excretion of exogenous BSP-GSH was significantly reduced. TC also diminished the depressing effect of BSP on state III respiration of liver mitochondria in vitro. It is concluded that this effect of TC may play a role also in the increased biliary excretion of intraphepatic conjugated BSP.", "contents": "Effect of sodium taurocholate on the hepatic transport of bromsulphthalein in rats. In anaesthetized, bile duct-cannulated rats the hepatic transport of bromsulphthalein (BSP) showed a dose-dependent increase in response to simultaneous administration of taurocholate (TC). The excretion rates of both free (BSP) and conjugated bromsulphthalein (BSP-GSH) were significantly elevated by TC. The simultaneously given TC decreased the hepatic uptake of BSP and BSP-GSH, but did not influence the conjugation of BSP with glutathione (GSH) or the biliary excretion rate of exogenous BSP-GSH. When the liver was depleted of GSH by pretreatment with diethyl-maleate (DEM), TC increased the excretion of free BSP. In DEM-treated rats free BSP markedly depressed the biliary excretion of exogenous BSP-GSH. When TC was given simultaneously with BSP, the inhibitory effect of BSP on the excretion of exogenous BSP-GSH was significantly reduced. TC also diminished the depressing effect of BSP on state III respiration of liver mitochondria in vitro. It is concluded that this effect of TC may play a role also in the increased biliary excretion of intraphepatic conjugated BSP."}
{"id": "PMID:485697", "title": "Effect of anesthetics on mortality and kidney lesions caused by hypotension.", "content": "Unanesthetized rats and rats anesthetized with a number of anesthetics were bled according to different bleeding schedules. In rats bled for 60 min against 30-50 mm Hg marked protection against mortality and the occurrence of kidney lesions was obtained with pentobarbital-Na, enflurane, halothane and methoxyflurane. Less protection was provided by diethylether while little or no protection was obtained with ethylurethane, trichloroethylene and ketamine. Protection was afforded by pentobarbital-Na in the dose range of 50 mg/kg to 6.2 mg/kg. Slight protection was afforded by dehydrobenzperidol. If pentobarbital-Na was administered after the bleeding the protection was slight. At bleeding pressures below 30 mm Hg the protective effect disappeared for all anesthetics used. Protection was not correlated with either depth of anesthesia, hypotensive effect or reduction of the maximum bleeding volume. However, a correlation was found between protective effect and the retardation of bleeding and the prolongation of survival time caused by the different anesthetics.", "contents": "Effect of anesthetics on mortality and kidney lesions caused by hypotension. Unanesthetized rats and rats anesthetized with a number of anesthetics were bled according to different bleeding schedules. In rats bled for 60 min against 30-50 mm Hg marked protection against mortality and the occurrence of kidney lesions was obtained with pentobarbital-Na, enflurane, halothane and methoxyflurane. Less protection was provided by diethylether while little or no protection was obtained with ethylurethane, trichloroethylene and ketamine. Protection was afforded by pentobarbital-Na in the dose range of 50 mg/kg to 6.2 mg/kg. Slight protection was afforded by dehydrobenzperidol. If pentobarbital-Na was administered after the bleeding the protection was slight. At bleeding pressures below 30 mm Hg the protective effect disappeared for all anesthetics used. Protection was not correlated with either depth of anesthesia, hypotensive effect or reduction of the maximum bleeding volume. However, a correlation was found between protective effect and the retardation of bleeding and the prolongation of survival time caused by the different anesthetics."}
{"id": "PMID:485698", "title": "Contraction of cat nictitating membrane by tetramisole.", "content": "Tetramisole produced a dose-dependent persistent contraction of cat nictitating membrane. This was not significantly altered by prior adrenalectomy or reserpinisation and tyraminisation, but was abolished by prior alpha blockade.", "contents": "Contraction of cat nictitating membrane by tetramisole. Tetramisole produced a dose-dependent persistent contraction of cat nictitating membrane. This was not significantly altered by prior adrenalectomy or reserpinisation and tyraminisation, but was abolished by prior alpha blockade."}
{"id": "PMID:485699", "title": "Effect of pentobarbital and urethane on the oxygen tension in the kidney of hypotensive rats.", "content": "The effect of bleeding against a predetermined pressure on the relative oxygen tension in the kidney was studied in rats anesthetized with either pentobarbital-Na or ethylurethane. In rats bled either for 30 min against 40 mm Hg or for 60 min against 50 mm Hg, the fall in the relative oxygen tension was significantly less during anesthesia with pentobarbital-Na, a highly potent protector against the occurrence of kidney lesions, than during anesthesia with ethylurethane, which possesses only slight protective potency. When rats anesthetized with ethylurethane were allowed to breathe oxygen the relative oxygen tension in the kidney was increased only for 15 to 20 min and accordingly the severity of the kidney lesions was decreased only when the animals were bled for 30 min at 40 mm Hg. When a number of different bleeding schedules were applied, a correlation was found between the average relative oxygen tension during bleeding and the incidence and degree of kidney lesions observed, irrespective of either the bleeding schedule or the anesthetic used. It is concluded that hypoxia may be a major factor in determining the occurrence of kidney lesions after bleeding and that the protective effect of anesthetics depends on their ability to diminish the fall in oxygen tension in the kidney during bleeding.", "contents": "Effect of pentobarbital and urethane on the oxygen tension in the kidney of hypotensive rats. The effect of bleeding against a predetermined pressure on the relative oxygen tension in the kidney was studied in rats anesthetized with either pentobarbital-Na or ethylurethane. In rats bled either for 30 min against 40 mm Hg or for 60 min against 50 mm Hg, the fall in the relative oxygen tension was significantly less during anesthesia with pentobarbital-Na, a highly potent protector against the occurrence of kidney lesions, than during anesthesia with ethylurethane, which possesses only slight protective potency. When rats anesthetized with ethylurethane were allowed to breathe oxygen the relative oxygen tension in the kidney was increased only for 15 to 20 min and accordingly the severity of the kidney lesions was decreased only when the animals were bled for 30 min at 40 mm Hg. When a number of different bleeding schedules were applied, a correlation was found between the average relative oxygen tension during bleeding and the incidence and degree of kidney lesions observed, irrespective of either the bleeding schedule or the anesthetic used. It is concluded that hypoxia may be a major factor in determining the occurrence of kidney lesions after bleeding and that the protective effect of anesthetics depends on their ability to diminish the fall in oxygen tension in the kidney during bleeding."}
{"id": "PMID:485700", "title": "Action of glomerulopressin on smooth muscle contraction, probably mediated by the release of prostaglandins.", "content": "The effect of glomerulopressin on the increase of the contractile tension developed by isolated strips of stomach fundus, duodenum and bladder of rats, was investigated. Glomerulopressin is a glucuronide whose contractile inducing effect was destroyed by beta-glucuronidase. It was observed that glomerulopressin increases the contractile tension of these tissues, and that this action was not blocked by atropine, cyproheptadine, tranylcypromine, but was inhibited by indomethacin, suggesting that the effect of glomerulopressin action was mediated by prostaglandin release.", "contents": "Action of glomerulopressin on smooth muscle contraction, probably mediated by the release of prostaglandins. The effect of glomerulopressin on the increase of the contractile tension developed by isolated strips of stomach fundus, duodenum and bladder of rats, was investigated. Glomerulopressin is a glucuronide whose contractile inducing effect was destroyed by beta-glucuronidase. It was observed that glomerulopressin increases the contractile tension of these tissues, and that this action was not blocked by atropine, cyproheptadine, tranylcypromine, but was inhibited by indomethacin, suggesting that the effect of glomerulopressin action was mediated by prostaglandin release."}
{"id": "PMID:485701", "title": "A cholecystokinin-like peptide in crude substance P from human and bovine brain.", "content": "The subfractions of crude substance P (SP), Fa, Fb, and Fc, were assayed for SP-like activity (SP-LA) and cholecystokinin octapeptide-like activity (CCK-8-LA) using radioimmunoassays (RIAs) and bioassays. Crude SP preparations from total human and bovine brains, cortex, and subcortex (amygdala, substantia nigra) contain CCK-8-LA as well as SP-LA. Most of SP-LA was contained in Fb, whereas most of CCK-8-LA was in Fc. Fa gave ambiguous results. Pharmacological results previously obtained with Fc may reflect the activity of CCK-like peptide(s).", "contents": "A cholecystokinin-like peptide in crude substance P from human and bovine brain. The subfractions of crude substance P (SP), Fa, Fb, and Fc, were assayed for SP-like activity (SP-LA) and cholecystokinin octapeptide-like activity (CCK-8-LA) using radioimmunoassays (RIAs) and bioassays. Crude SP preparations from total human and bovine brains, cortex, and subcortex (amygdala, substantia nigra) contain CCK-8-LA as well as SP-LA. Most of SP-LA was contained in Fb, whereas most of CCK-8-LA was in Fc. Fa gave ambiguous results. Pharmacological results previously obtained with Fc may reflect the activity of CCK-like peptide(s)."}
{"id": "PMID:485702", "title": "Cholinergic potentiation by ouabain in the contraction of guinea-pig vas deferens through pre- and post-synaptic mechanism.", "content": "In the guinea-pig vas deferens, the contractions induced by pilocarpine and arecoline, unlike ACh, were markedly reduced by treatment with procaine (10(-6) g/ml) or hemicholinium (5 x 10(-4) g/ml). These reductions by hemicholinium were completely recovered after exposure to choline. The contractile responses to these three agonists were eliminated by atropine (3 x 10(-8) g/ml) but enhanced by neostigmine (3 x 10(-8) g/ml), though these were not affected at all by tetrodotoxin (3 x 10(-8) g/ml) or hexamethonium (10(-5) g/ml). The contractile responses to ACh, pilocarpine and arecoline were potentiated by ouabain (10(-6) g/ml). These potentiations were more marked in pilocarpine and arecoline than those in ACh. The potentiation in response to arecoline was maximal after incubation with ouabain for 5 min but that to ACh for 45 min. The results suggest that pilocarpine and arecoline act as ACh-releaser in this tissue and that the ouabain-induced cholinergic potentiation may be attributable to a dual mechanism, facilitation of ACh release at presynaptic site and alteration of the membrane excitability at postsynaptic site.", "contents": "Cholinergic potentiation by ouabain in the contraction of guinea-pig vas deferens through pre- and post-synaptic mechanism. In the guinea-pig vas deferens, the contractions induced by pilocarpine and arecoline, unlike ACh, were markedly reduced by treatment with procaine (10(-6) g/ml) or hemicholinium (5 x 10(-4) g/ml). These reductions by hemicholinium were completely recovered after exposure to choline. The contractile responses to these three agonists were eliminated by atropine (3 x 10(-8) g/ml) but enhanced by neostigmine (3 x 10(-8) g/ml), though these were not affected at all by tetrodotoxin (3 x 10(-8) g/ml) or hexamethonium (10(-5) g/ml). The contractile responses to ACh, pilocarpine and arecoline were potentiated by ouabain (10(-6) g/ml). These potentiations were more marked in pilocarpine and arecoline than those in ACh. The potentiation in response to arecoline was maximal after incubation with ouabain for 5 min but that to ACh for 45 min. The results suggest that pilocarpine and arecoline act as ACh-releaser in this tissue and that the ouabain-induced cholinergic potentiation may be attributable to a dual mechanism, facilitation of ACh release at presynaptic site and alteration of the membrane excitability at postsynaptic site."}
{"id": "PMID:485703", "title": "Pharmacological evidence of an interaction between serotonergic and dopaminergic neurons in the control of prolactin secretion in male rats.", "content": "The purpose of the present study was to investigate the possible interaction between serotonergic and dopaminergic neurons in regulating prolactin (PRL) secretion. We have examined the effect of quipazine, a drug which has been reported to increase plasma PRL levels by acting through a serotonergic mechanism, on PRL release in rats pretreated with penfluridol or alpha-methyl-p-tyrosine (alpha-MPT). The effect of d-fenfluramine, a serotonin releaser, on plasma PRL levels in animals pretreated with penfluridol was also studied. Penfluridol or alpha-MPT treatments significantly stimulated PRL secretion. Quipazine also increased plasma PRL levels in normal male rats. However, this drug was not able to further stimulate PRL release in animals pretreated with penfluridol or alpha-MPT. Like quipazine, d-fenfluramine increased plasma PRL levels in normal rats but it failed to further stimulate PRL secretion in penfluridol-pretreated animals. These findings support the hypothesis that serotonin may stimulate PRL release through an inhibition of dopaminergic neurons.", "contents": "Pharmacological evidence of an interaction between serotonergic and dopaminergic neurons in the control of prolactin secretion in male rats. The purpose of the present study was to investigate the possible interaction between serotonergic and dopaminergic neurons in regulating prolactin (PRL) secretion. We have examined the effect of quipazine, a drug which has been reported to increase plasma PRL levels by acting through a serotonergic mechanism, on PRL release in rats pretreated with penfluridol or alpha-methyl-p-tyrosine (alpha-MPT). The effect of d-fenfluramine, a serotonin releaser, on plasma PRL levels in animals pretreated with penfluridol was also studied. Penfluridol or alpha-MPT treatments significantly stimulated PRL secretion. Quipazine also increased plasma PRL levels in normal male rats. However, this drug was not able to further stimulate PRL release in animals pretreated with penfluridol or alpha-MPT. Like quipazine, d-fenfluramine increased plasma PRL levels in normal rats but it failed to further stimulate PRL secretion in penfluridol-pretreated animals. These findings support the hypothesis that serotonin may stimulate PRL release through an inhibition of dopaminergic neurons."}
{"id": "PMID:485704", "title": "Effects of intraventricular and intraspinal 6-hydroxydopamine on blood pressure of DOCA-saline hypertensive rats.", "content": "The effects of intraventricular and intraspinal administration of 6-hydroxydopamine (6-OH-DA) on the development and maintenance of DOCA-saline hypertension in rats have been investigated. Intraventricular administration of 6-OH-DA prevented the development of DOCA-saline hypertension in rats, but was ineffective in developed DOCA-saline hypertension. Treatment with intraventricular 6-OH-DA on rise in blood pressure was not secondary to a reduction in salt intake, however, since vehicle-treated rats given a similar reduced salt intake became fully hypertensive. The development of DOCA-saline hypertension was unaffected by pretreatment with intraspinal administration of 6-OH-DA, which produced a virtually complete loss of noradrenaline only in the spinal cord. It is suggested that brain adrenergic neurones may participate in the production of DOCA-saline hypertension but the noradrenergic projections in the spinal cords are not essential for this process.", "contents": "Effects of intraventricular and intraspinal 6-hydroxydopamine on blood pressure of DOCA-saline hypertensive rats. The effects of intraventricular and intraspinal administration of 6-hydroxydopamine (6-OH-DA) on the development and maintenance of DOCA-saline hypertension in rats have been investigated. Intraventricular administration of 6-OH-DA prevented the development of DOCA-saline hypertension in rats, but was ineffective in developed DOCA-saline hypertension. Treatment with intraventricular 6-OH-DA on rise in blood pressure was not secondary to a reduction in salt intake, however, since vehicle-treated rats given a similar reduced salt intake became fully hypertensive. The development of DOCA-saline hypertension was unaffected by pretreatment with intraspinal administration of 6-OH-DA, which produced a virtually complete loss of noradrenaline only in the spinal cord. It is suggested that brain adrenergic neurones may participate in the production of DOCA-saline hypertension but the noradrenergic projections in the spinal cords are not essential for this process."}
{"id": "PMID:485705", "title": "Acute effects of opiate agonists, partial agonists and antagonists on brain monoamines in the rat.", "content": "Single doses of 4.5 mg/kg of the opiate agonist, morphine, the partial agonists, cyclazocine, pentazocine, levallorphan and nalorphine, the antagonists, naloxone and naltrexone and dextrorphan were injected i.p. in groups of rats. Behaviour was noted for 30 min following injection and at this time, brains were removed and levels of noradrenaline (NA), dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) were determined spectrophotofluorimetrically. Only morphine and cyclazocine altered behaviour and 5-HIAA was the only substance which was consistently elevated by all the compounds tested. These results indicate that a causal relationship between the acute effects of opiate agonists, partial agonists and antagonists on brain 5-HT metabolism and their pharmacological actions is unlikely.", "contents": "Acute effects of opiate agonists, partial agonists and antagonists on brain monoamines in the rat. Single doses of 4.5 mg/kg of the opiate agonist, morphine, the partial agonists, cyclazocine, pentazocine, levallorphan and nalorphine, the antagonists, naloxone and naltrexone and dextrorphan were injected i.p. in groups of rats. Behaviour was noted for 30 min following injection and at this time, brains were removed and levels of noradrenaline (NA), dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) were determined spectrophotofluorimetrically. Only morphine and cyclazocine altered behaviour and 5-HIAA was the only substance which was consistently elevated by all the compounds tested. These results indicate that a causal relationship between the acute effects of opiate agonists, partial agonists and antagonists on brain 5-HT metabolism and their pharmacological actions is unlikely."}
{"id": "PMID:485706", "title": "Pharmacological and biochemical studies on a new compound, 2-(7-indeyloxymethyl)morpholine hydrochloride (YM-08054-1), and its derivatives with potential antidepressant properties.", "content": "Antidepressant properties of a new indene derivative, YM-08054-1, and its related compounds were compared with those of tricyclic antidepressants and viloxazine. The potencies of YM-08054-1 to inhibit uptake of both 14C-norepinephrine (14C-NE) and 14C-5-hydroxytryptamine (14C-5-HT) by the rat brain synaptosomes were similar to those of amitriptyline and imipramine. Other indene derivatives with an N-alkylated morpholine ring were proved to have less effect on the uptake of either 5-HT or NE than was YM-08054-1. YM-08054-1 was the most potent among all of the tested antidepressants in the inhibition of reserpine-induced facilitation of convulsions as well as in the potentiation of reserpine-induced facilitation of convulsions as well as in the potentiation of 5-hydroxytryptophan (5-HTP)-induced syndromes in mice, though the inhibitory effect of this agent on reserpine-induced hypothermia was weaker than that of either amitriptyline or desipramine, suggesting relatively selective effects of YM-08054-1 upon 5-HT rather than NE uptake in vivo. Neither viloxazine nor iprindole potentiated the responses to 5-HTP. YM-08054-1 was devoid of peripheral anticholinergic activity and exhibited weak local anesthetic effect as well as low acute toxicity when compared with amitriptyline. The results indicate that YM-08054-1 has a novel profile as an antidepressant agent which is quite different from that of either viloxazine or tricyclic compounds.", "contents": "Pharmacological and biochemical studies on a new compound, 2-(7-indeyloxymethyl)morpholine hydrochloride (YM-08054-1), and its derivatives with potential antidepressant properties. Antidepressant properties of a new indene derivative, YM-08054-1, and its related compounds were compared with those of tricyclic antidepressants and viloxazine. The potencies of YM-08054-1 to inhibit uptake of both 14C-norepinephrine (14C-NE) and 14C-5-hydroxytryptamine (14C-5-HT) by the rat brain synaptosomes were similar to those of amitriptyline and imipramine. Other indene derivatives with an N-alkylated morpholine ring were proved to have less effect on the uptake of either 5-HT or NE than was YM-08054-1. YM-08054-1 was the most potent among all of the tested antidepressants in the inhibition of reserpine-induced facilitation of convulsions as well as in the potentiation of reserpine-induced facilitation of convulsions as well as in the potentiation of 5-hydroxytryptophan (5-HTP)-induced syndromes in mice, though the inhibitory effect of this agent on reserpine-induced hypothermia was weaker than that of either amitriptyline or desipramine, suggesting relatively selective effects of YM-08054-1 upon 5-HT rather than NE uptake in vivo. Neither viloxazine nor iprindole potentiated the responses to 5-HTP. YM-08054-1 was devoid of peripheral anticholinergic activity and exhibited weak local anesthetic effect as well as low acute toxicity when compared with amitriptyline. The results indicate that YM-08054-1 has a novel profile as an antidepressant agent which is quite different from that of either viloxazine or tricyclic compounds."}
{"id": "PMID:485707", "title": "Effects of ethanol and acetaldehyde on the isolated, blood-perfused canine atrium.", "content": "In the isolated, blood-perfused canine atrium preparations, ethanol injected into the cannulated sinus node artery consistently produced dose-dependently negative inotropic effects at a dose range of 0.03 to 1 mmol and negative chronotropic effects starting from 0.1 mmol. Ethanol-induced negative inotropic and chronotropic responses were not blocked by an adequate dose of atropine. When acetaldehyde was given into the sinus node artery, positive inotropic and chronotropic effects were dose-relatedly produced from 0.1 to 30 mumol. Acetaldehyde-induced positive inotropic and chronotropic effects were inhibited by treatment with propranolol. However, these positive effects were not suppressed by either imipramine or tetrodotoxin, although imipramine inhibited tyramine-induced actions and tetrodotoxin suppressed acetylcholine-induced positive chronotropic and inotropic effects in atropine-treated preparations. From these results, it is suggested that ethanol has direct depressant properties on pacemaker and contractile activities, and that acetaldehyde has stimulating properties through adrenergic beta-receptors but neither through tyramine-like action nor nerve excitation.", "contents": "Effects of ethanol and acetaldehyde on the isolated, blood-perfused canine atrium. In the isolated, blood-perfused canine atrium preparations, ethanol injected into the cannulated sinus node artery consistently produced dose-dependently negative inotropic effects at a dose range of 0.03 to 1 mmol and negative chronotropic effects starting from 0.1 mmol. Ethanol-induced negative inotropic and chronotropic responses were not blocked by an adequate dose of atropine. When acetaldehyde was given into the sinus node artery, positive inotropic and chronotropic effects were dose-relatedly produced from 0.1 to 30 mumol. Acetaldehyde-induced positive inotropic and chronotropic effects were inhibited by treatment with propranolol. However, these positive effects were not suppressed by either imipramine or tetrodotoxin, although imipramine inhibited tyramine-induced actions and tetrodotoxin suppressed acetylcholine-induced positive chronotropic and inotropic effects in atropine-treated preparations. From these results, it is suggested that ethanol has direct depressant properties on pacemaker and contractile activities, and that acetaldehyde has stimulating properties through adrenergic beta-receptors but neither through tyramine-like action nor nerve excitation."}
{"id": "PMID:485708", "title": "Possible involvement of indirect mechanism in cardiovascular action of dobutamine in dogs.", "content": "Possible mechanisms of dobutamine effects on adrenoceptors were investigated in comparison with tyramine, dopamine and noradrenaline after depletion of noradrenaline stores by ephedrine in dogs. Dobutamine, like other amines, induced pressor and positive chronotropic effects. After repeated treatment with ephedrine (total 40 mg/kg), both effects of dobutamine were markedly depressed. However, after ephedrine, the effects of tyramine were eliminated while those of noradrenaline were slightly decreased. Pressor responses to dopamine were reversed to depressor and positive chronotropic responses were abolished. The results suggest that dobutamine differs from other catecholamines in its mode of action and that its cardiovascular actions may be mediated in part through an indirect action on adrenoceptors.", "contents": "Possible involvement of indirect mechanism in cardiovascular action of dobutamine in dogs. Possible mechanisms of dobutamine effects on adrenoceptors were investigated in comparison with tyramine, dopamine and noradrenaline after depletion of noradrenaline stores by ephedrine in dogs. Dobutamine, like other amines, induced pressor and positive chronotropic effects. After repeated treatment with ephedrine (total 40 mg/kg), both effects of dobutamine were markedly depressed. However, after ephedrine, the effects of tyramine were eliminated while those of noradrenaline were slightly decreased. Pressor responses to dopamine were reversed to depressor and positive chronotropic responses were abolished. The results suggest that dobutamine differs from other catecholamines in its mode of action and that its cardiovascular actions may be mediated in part through an indirect action on adrenoceptors."}
{"id": "PMID:485709", "title": "Pentagastrin stimulates tonic and phasic contractile activity of the canine stomach.", "content": "Seven strain gage force transducers were sutured in line to the stomach of an anesthetized dog. Quantification of tonic acitivity and of phasic activity (amplitude, frequency and propagation velocity) on 7 locations from fundus to pylorus resulted in an overall picture of gastric motor activity. Pentagastrin (dose-response curve, 1--8192 ng/kg, i.v.) did not change propagation velocity of the contractile waves, but increased the amplitude and frequency of phasic activity on corpus and antrum. Amplitude increased on the antrum more than on the corpus. Frequency increase was uniform over corpus and antrum. Moreover pentagastrin increased muscle tone (tonic activity) in a specific area on the corpus from 12--18 cm to pylorus. The distinct responses to pentagastrin of tonic activity and of amplitude and frequency of phasic activity indicate that pentagastrin acts on 3 different receptor-effector systems.", "contents": "Pentagastrin stimulates tonic and phasic contractile activity of the canine stomach. Seven strain gage force transducers were sutured in line to the stomach of an anesthetized dog. Quantification of tonic acitivity and of phasic activity (amplitude, frequency and propagation velocity) on 7 locations from fundus to pylorus resulted in an overall picture of gastric motor activity. Pentagastrin (dose-response curve, 1--8192 ng/kg, i.v.) did not change propagation velocity of the contractile waves, but increased the amplitude and frequency of phasic activity on corpus and antrum. Amplitude increased on the antrum more than on the corpus. Frequency increase was uniform over corpus and antrum. Moreover pentagastrin increased muscle tone (tonic activity) in a specific area on the corpus from 12--18 cm to pylorus. The distinct responses to pentagastrin of tonic activity and of amplitude and frequency of phasic activity indicate that pentagastrin acts on 3 different receptor-effector systems."}
{"id": "PMID:485710", "title": "The effects of ritodrine on experimentally induced intra-uterine growth retardation in the rat.", "content": "The effects of oral ritodrine on artificially induced intra-uterine growth retardation in rats were tested in two animal models. In one model, foetal hypotrophy was induced by reduction of the uterine blood supply on ligating of the left uterine artery. In that experiment no significant inhibition of the foetal hypotrophy could be detected in the ritodrine treated animals, but the corresponding reduction of placental weight was inhibited. In the other model, hypotrophy was induced by hypothermia. Oral ritodrine, given twice daily in a dose of 50 mg/kg from day 7 to day 21 of the pregnancy, antagonized the decrease of the foetal weight produced by hypothermia.", "contents": "The effects of ritodrine on experimentally induced intra-uterine growth retardation in the rat. The effects of oral ritodrine on artificially induced intra-uterine growth retardation in rats were tested in two animal models. In one model, foetal hypotrophy was induced by reduction of the uterine blood supply on ligating of the left uterine artery. In that experiment no significant inhibition of the foetal hypotrophy could be detected in the ritodrine treated animals, but the corresponding reduction of placental weight was inhibited. In the other model, hypotrophy was induced by hypothermia. Oral ritodrine, given twice daily in a dose of 50 mg/kg from day 7 to day 21 of the pregnancy, antagonized the decrease of the foetal weight produced by hypothermia."}
{"id": "PMID:485711", "title": "Effect of monofluoroacetate on renal H+ excretion in the rat.", "content": "In order to investigate the effect of monofluoroacetate (MFA) on renal H+ excretion, anesthetized rats under mannitol diuresis were given intraperitoneally MFA and some of the acido-basic status parameters were determined. Urinary pH and pCO2 did not change after MFA administration, while urinary flow rate increased. MFA induced a decrease in H+ net excretion and in ammonia excretion. Titratable acidity did not change significantly within the experiment.", "contents": "Effect of monofluoroacetate on renal H+ excretion in the rat. In order to investigate the effect of monofluoroacetate (MFA) on renal H+ excretion, anesthetized rats under mannitol diuresis were given intraperitoneally MFA and some of the acido-basic status parameters were determined. Urinary pH and pCO2 did not change after MFA administration, while urinary flow rate increased. MFA induced a decrease in H+ net excretion and in ammonia excretion. Titratable acidity did not change significantly within the experiment."}
{"id": "PMID:485712", "title": "Decrease in hepatic blood flow during furosemide-induced diuresis.", "content": "The effect of furosemide on hepatic hemodynamics was investigated using electromagnetic flow probes positioned around the hepatic-portal vein and common hepatic artery of anesthetized dogs. Furosemide administration induced a vigorous diuresis and concomitantly decreased total hepatic blood flow. Hepatic arterial blood remained relatively stable. Thus, the decrease in total hepatic blood flow was due primarily to a decrease in portal blood flow. When furosemide was given to animals with ureters ligated to prevent extracellular volume contraction, the drug did not reduce total hepatic blood flow, portal blood flow or hepatic arterial blood flow. Thus, furosemide induces a decrease in total hepatic blood flow that appears to involve a mechanism dependent upon the volume contraction produced by the diuretic. The contraction causes a reduction in venous blood flow but not arterial blood flow to the liver.", "contents": "Decrease in hepatic blood flow during furosemide-induced diuresis. The effect of furosemide on hepatic hemodynamics was investigated using electromagnetic flow probes positioned around the hepatic-portal vein and common hepatic artery of anesthetized dogs. Furosemide administration induced a vigorous diuresis and concomitantly decreased total hepatic blood flow. Hepatic arterial blood remained relatively stable. Thus, the decrease in total hepatic blood flow was due primarily to a decrease in portal blood flow. When furosemide was given to animals with ureters ligated to prevent extracellular volume contraction, the drug did not reduce total hepatic blood flow, portal blood flow or hepatic arterial blood flow. Thus, furosemide induces a decrease in total hepatic blood flow that appears to involve a mechanism dependent upon the volume contraction produced by the diuretic. The contraction causes a reduction in venous blood flow but not arterial blood flow to the liver."}
{"id": "PMID:485713", "title": "Induction of disopyramide N-dealkylation by phenobarbital and disopyramide in rat liver.", "content": "An in vitro assay for the determination of the activity of disopyramide-N-dealkylation was developed. This reaction was concluded to be catalyzed by the liver microsomal, cytochrome P-450 centered monooxygenase system. Phenobarbital enhanced the N-dealkylation of disopyramide four fold, and disopyramide itself 1.6 fold, whereas methylcholanthrene was without effect. Disopyramide also increased ethoxycoumarin deethylation 1.6 fold, and had a slight increasing effect on the activity of epoxide hydratase, but did not affect the activities of glutathione S-transferase or UDPglucuronosyltransferase.", "contents": "Induction of disopyramide N-dealkylation by phenobarbital and disopyramide in rat liver. An in vitro assay for the determination of the activity of disopyramide-N-dealkylation was developed. This reaction was concluded to be catalyzed by the liver microsomal, cytochrome P-450 centered monooxygenase system. Phenobarbital enhanced the N-dealkylation of disopyramide four fold, and disopyramide itself 1.6 fold, whereas methylcholanthrene was without effect. Disopyramide also increased ethoxycoumarin deethylation 1.6 fold, and had a slight increasing effect on the activity of epoxide hydratase, but did not affect the activities of glutathione S-transferase or UDPglucuronosyltransferase."}
{"id": "PMID:485717", "title": "Bilirubin excretion pattern in manganese-bilirubin cholestasis.", "content": "Impaired bilirubin excretion has been investigated as the possible mechanism for the cholestasis observed following the injection of manganese and bilirubin in rats. We studied the biliary excretion pattern of bilirubin in bile under both cholestatic (manganese plus bilirubin) and noncholestatic (manganese or bilirubin alone; manganese plus bilirubin plus sulfobromophthalein) conditions using a diazotization reaction and this layer chromatography. The accumulation of non-C-1-glucuronides or of nonglucuronoconjugates of bilirubin in bile does not seem to be responsible for the cholestasis. The altered biliary bilirubin metabolic pattern of the azodipyrollic fractions observed during manganese-bilirubin cholestasis appeared to be the result of the cholestasis, rather than its cause.", "contents": "Bilirubin excretion pattern in manganese-bilirubin cholestasis. Impaired bilirubin excretion has been investigated as the possible mechanism for the cholestasis observed following the injection of manganese and bilirubin in rats. We studied the biliary excretion pattern of bilirubin in bile under both cholestatic (manganese plus bilirubin) and noncholestatic (manganese or bilirubin alone; manganese plus bilirubin plus sulfobromophthalein) conditions using a diazotization reaction and this layer chromatography. The accumulation of non-C-1-glucuronides or of nonglucuronoconjugates of bilirubin in bile does not seem to be responsible for the cholestasis. The altered biliary bilirubin metabolic pattern of the azodipyrollic fractions observed during manganese-bilirubin cholestasis appeared to be the result of the cholestasis, rather than its cause."}
{"id": "PMID:485719", "title": "Metronidazole: a comparative study on the intravaginal absorption, metabolism and disposition of a single product and commercial formulation.", "content": "The absorption, tissue distribution, metabolism and excretion of 14C-metronidazole (14C-MTZ) were compared during the first 24 hr upon the intravaginal (ivg) administration of 14C-MTZ, either as a single product (ivg-sp) or as a formulation in the form of a commercial cream (ivg-cp). Peak 14C levels in tail blood samples were reached at 1 and 2 hr in ivg-sp and ivg-cp treated rats, respectively. The rate of decline of blood 14C was faster in the ivg-sp treated rats as opposed to their ivg-cp treated counterparts. At the end of 24 hr, the highest 14C concentrations were observed in the kidneys and livers in both treatment groups. While the liver, kidney and lung concentrations of 14C were similar in both groups, the blood, plasma, brain, fat and skeletal muscle levels of 14C were significantly greater in the ivg-cp treated rats as compared with the ivg-sp treated animals. The combined excretion of radio-activity in the urine and feces of ivg-sp treated rats was significantly greater (77.7 +/- 3.0 vs. 58.0 +/- 2.0) than that of the ivg-cp treated animals. Irrespective of the product applied, about 1% of the applied dose remained in the vagina at the end of 24 hr. Unchanged 14C-MTZ and its five metabolites were detected from 0-12 and 12.24 hr urines of both groups. In comparison with the ivg-cp treated rats, the amount of unchanged 14C-MTZ was significantly greater in 0-12 hr urines of ivg-sp treated rats, whereas a reverse situation was observed during 12-24 hr period. The results of this study show that both products are almost completely absorbed through the vaginal mucosa of the rat; however, the kinetics of MTZ-derived radioactivity are different following the administration of equal amounts of each product.", "contents": "Metronidazole: a comparative study on the intravaginal absorption, metabolism and disposition of a single product and commercial formulation. The absorption, tissue distribution, metabolism and excretion of 14C-metronidazole (14C-MTZ) were compared during the first 24 hr upon the intravaginal (ivg) administration of 14C-MTZ, either as a single product (ivg-sp) or as a formulation in the form of a commercial cream (ivg-cp). Peak 14C levels in tail blood samples were reached at 1 and 2 hr in ivg-sp and ivg-cp treated rats, respectively. The rate of decline of blood 14C was faster in the ivg-sp treated rats as opposed to their ivg-cp treated counterparts. At the end of 24 hr, the highest 14C concentrations were observed in the kidneys and livers in both treatment groups. While the liver, kidney and lung concentrations of 14C were similar in both groups, the blood, plasma, brain, fat and skeletal muscle levels of 14C were significantly greater in the ivg-cp treated rats as compared with the ivg-sp treated animals. The combined excretion of radio-activity in the urine and feces of ivg-sp treated rats was significantly greater (77.7 +/- 3.0 vs. 58.0 +/- 2.0) than that of the ivg-cp treated animals. Irrespective of the product applied, about 1% of the applied dose remained in the vagina at the end of 24 hr. Unchanged 14C-MTZ and its five metabolites were detected from 0-12 and 12.24 hr urines of both groups. In comparison with the ivg-cp treated rats, the amount of unchanged 14C-MTZ was significantly greater in 0-12 hr urines of ivg-sp treated rats, whereas a reverse situation was observed during 12-24 hr period. The results of this study show that both products are almost completely absorbed through the vaginal mucosa of the rat; however, the kinetics of MTZ-derived radioactivity are different following the administration of equal amounts of each product."}
{"id": "PMID:485720", "title": "Effects of cytidine-5' diphosphocholine on norepinephrine, dopamine and serotonin synthesis in various regions of the rat brain.", "content": "Administered intravenously to rats, CDPcholine, significantly increases the level and the synthesis rate of dopamine, and the level of tyrosine in the corpus striatum (maximum effect for 50 mg/kg, one hour after administration). CDPcholine decreases the level of serotonin and tryptophan and the synthesis rate of serotonin in the midbrain + hypothalamus and in the brain stem. The increase of the striatum dopamine level and the decrease of the brain stem and midbrain serotonin level are correlated with the recognized antiparkinson and neurostimulant action of this nucleotide.", "contents": "Effects of cytidine-5' diphosphocholine on norepinephrine, dopamine and serotonin synthesis in various regions of the rat brain. Administered intravenously to rats, CDPcholine, significantly increases the level and the synthesis rate of dopamine, and the level of tyrosine in the corpus striatum (maximum effect for 50 mg/kg, one hour after administration). CDPcholine decreases the level of serotonin and tryptophan and the synthesis rate of serotonin in the midbrain + hypothalamus and in the brain stem. The increase of the striatum dopamine level and the decrease of the brain stem and midbrain serotonin level are correlated with the recognized antiparkinson and neurostimulant action of this nucleotide."}
{"id": "PMID:485721", "title": "Activity of some drugs on the electroencephalogram of curarized rats during acute and iterative hypercapnic anoxia Hypotheses on mechanisms of action.", "content": "The present study is related to the activity of some drugs in a test of acute and iterative hypercapnic anoxia in curarized rats. The interpretation of the results is based on the values of cerebral resistance to anoxia and on the duration of post-anoxic recovery and cerebral electric silence, i.e. the duration of \"nul\" electroencephalogram. Results obtained allow us to put forward some hypotheses about mechanisms of action. Cerebral or systemic vasodilation or vasoconstriction both seem to be excluded. Membrane activity as well as an increase in cerebral blood perfusion pressure or an impact at the level of the brain neurotransmitters may be taken into consideration. Furthermore, it is possible that extracerebral phenomena (cardiovascular, respiratory, within the blood cells for example) could interact with cerebral phenomena. It would be necessary to undertake further, more extensive studies if we wish to evaluate the potential interest of the experimental model used and the way in which the drugs proposed as treatment for cerebrovascular diseases or ageing processes work.", "contents": "Activity of some drugs on the electroencephalogram of curarized rats during acute and iterative hypercapnic anoxia Hypotheses on mechanisms of action. The present study is related to the activity of some drugs in a test of acute and iterative hypercapnic anoxia in curarized rats. The interpretation of the results is based on the values of cerebral resistance to anoxia and on the duration of post-anoxic recovery and cerebral electric silence, i.e. the duration of \"nul\" electroencephalogram. Results obtained allow us to put forward some hypotheses about mechanisms of action. Cerebral or systemic vasodilation or vasoconstriction both seem to be excluded. Membrane activity as well as an increase in cerebral blood perfusion pressure or an impact at the level of the brain neurotransmitters may be taken into consideration. Furthermore, it is possible that extracerebral phenomena (cardiovascular, respiratory, within the blood cells for example) could interact with cerebral phenomena. It would be necessary to undertake further, more extensive studies if we wish to evaluate the potential interest of the experimental model used and the way in which the drugs proposed as treatment for cerebrovascular diseases or ageing processes work."}
{"id": "PMID:485722", "title": "Excitation of afferent fibres in the cardiac sympathetic nerves induced by coronary occlusion and injection of bradykinin. The influence of acetylsalicylic acid and dipyron.", "content": "Afferent impulse activity was recorded in single fibres of the inferior cardiac sympathetic nerve of the cat. When the descending branch of the left coronary artery was ligated for 60 sec an enhancement of afferent impulses was recorded. Elevations in discharge frequency were also induced by injecting bradykinin, epinephrine, and isoprenaline or by general hypoxia due to interruption of the artificial ventilation. When these procedures were after pretreatment with the analgesic agents, acetylsalicylic acid or dipyron a reduction in spike discharge was observed only with bradykinin after application of acetylsalicylic acid. No influence of these pretreatments on the effects of coronary occlusion, general hypoxia and injection of epinephrine and isoprenaline could be observed. These results suggest that bradykinin does not predominate as mediator substance in eliciting ischemic heart pain.", "contents": "Excitation of afferent fibres in the cardiac sympathetic nerves induced by coronary occlusion and injection of bradykinin. The influence of acetylsalicylic acid and dipyron. Afferent impulse activity was recorded in single fibres of the inferior cardiac sympathetic nerve of the cat. When the descending branch of the left coronary artery was ligated for 60 sec an enhancement of afferent impulses was recorded. Elevations in discharge frequency were also induced by injecting bradykinin, epinephrine, and isoprenaline or by general hypoxia due to interruption of the artificial ventilation. When these procedures were after pretreatment with the analgesic agents, acetylsalicylic acid or dipyron a reduction in spike discharge was observed only with bradykinin after application of acetylsalicylic acid. No influence of these pretreatments on the effects of coronary occlusion, general hypoxia and injection of epinephrine and isoprenaline could be observed. These results suggest that bradykinin does not predominate as mediator substance in eliciting ischemic heart pain."}
{"id": "PMID:485723", "title": "Interaction of cardiopulmonary chemoreceptor (Bezold-Jarisch) and somatosympathetic reflexes.", "content": "Cardiopulmonary chemoreceptor (Bezold-Jarisch) and baroreceptor reflexes produce similar overall cardiovascular effects although central integrating mechanisms are not identical. In view of recent reports emphasizing the complexity of baroreceptor mechanisms, the influence of both reflexes on spontaneous sympathetic outflow as well as their interaction with the somatosympathetic reflex were compared in anesthetized cats. The chemoreflex and the baroreceptor reflex were activated by i.v. injection of veratrine and norepinephrine, respectively, and both inhibited spontaneous efferent sympathetic nerve traffic. Stimulation of somatic nerves produces a two-component reflex potential in sympathetic nerves. Activation of cardiopulmonary chemoreceptor and baroreceptor reflexes inhibited the amplitude of the initial (spinal) phase of this somasympathetic reflex slightly and greatly attenuated the secondary (supraspinal) component. These experiments demonstrate that the cardiopulmonary chemoreceptor and baroreceptor reflexes exert similar effects on the somatosympathetic reflex and that the former two reflexes may influence sympathetic mechanisms at spinal as well as supraspinal levels.", "contents": "Interaction of cardiopulmonary chemoreceptor (Bezold-Jarisch) and somatosympathetic reflexes. Cardiopulmonary chemoreceptor (Bezold-Jarisch) and baroreceptor reflexes produce similar overall cardiovascular effects although central integrating mechanisms are not identical. In view of recent reports emphasizing the complexity of baroreceptor mechanisms, the influence of both reflexes on spontaneous sympathetic outflow as well as their interaction with the somatosympathetic reflex were compared in anesthetized cats. The chemoreflex and the baroreceptor reflex were activated by i.v. injection of veratrine and norepinephrine, respectively, and both inhibited spontaneous efferent sympathetic nerve traffic. Stimulation of somatic nerves produces a two-component reflex potential in sympathetic nerves. Activation of cardiopulmonary chemoreceptor and baroreceptor reflexes inhibited the amplitude of the initial (spinal) phase of this somasympathetic reflex slightly and greatly attenuated the secondary (supraspinal) component. These experiments demonstrate that the cardiopulmonary chemoreceptor and baroreceptor reflexes exert similar effects on the somatosympathetic reflex and that the former two reflexes may influence sympathetic mechanisms at spinal as well as supraspinal levels."}
{"id": "PMID:485724", "title": "Distribution and characterization of the adrenoceptors in dog coronary arteries.", "content": "Effects of catecholamines were studied in vitro on different portions of the left coronary artery of the dog. Adrenaline, noradrenaline and isoprenaline produced dose-related contractions in the common portion of the coronary artery which were blocked by phenoxybenzamine. The proximal portions were relaxed by noradrenaline and isoprenaline, but contracted or relaxed by adrenaline following an initial transient contraction. The contraction was blocked by phenoxybenzamine and the relaxation was blocked by propranolol. Adrenaline, noradrenaline and isoprenaline caused only relaxations which were blocked by propranolol in the distal portion and in the small branches. In coronary, femoral and tracheal strips contracted previously by KCl with or without a phenoxybenzamine pretreatment, isoprenaline produced dose-dependent relaxations. The dose-response curves for isoprenaline were shifted to the right on treatment with practolol. The blocking effects of practolol were nearly ten times potent in the coronary artery in comparison with the femoral artery and the trachea. The results indicate that the common portion mainly possesses alpha-adrenoceptors, the proximal portion contains both alpha- and beta-adrenoceptors, and that beta-adrenoceptors dominate in the distal portion and small artery branches. It is also shown that the beta-adrenoceptors in the coronary vessels differ from the femoral and tracheal beta-adrenoceptors in pharmacological feature.", "contents": "Distribution and characterization of the adrenoceptors in dog coronary arteries. Effects of catecholamines were studied in vitro on different portions of the left coronary artery of the dog. Adrenaline, noradrenaline and isoprenaline produced dose-related contractions in the common portion of the coronary artery which were blocked by phenoxybenzamine. The proximal portions were relaxed by noradrenaline and isoprenaline, but contracted or relaxed by adrenaline following an initial transient contraction. The contraction was blocked by phenoxybenzamine and the relaxation was blocked by propranolol. Adrenaline, noradrenaline and isoprenaline caused only relaxations which were blocked by propranolol in the distal portion and in the small branches. In coronary, femoral and tracheal strips contracted previously by KCl with or without a phenoxybenzamine pretreatment, isoprenaline produced dose-dependent relaxations. The dose-response curves for isoprenaline were shifted to the right on treatment with practolol. The blocking effects of practolol were nearly ten times potent in the coronary artery in comparison with the femoral artery and the trachea. The results indicate that the common portion mainly possesses alpha-adrenoceptors, the proximal portion contains both alpha- and beta-adrenoceptors, and that beta-adrenoceptors dominate in the distal portion and small artery branches. It is also shown that the beta-adrenoceptors in the coronary vessels differ from the femoral and tracheal beta-adrenoceptors in pharmacological feature."}
{"id": "PMID:485725", "title": "Role of extracellular calcium in isometric contractions of the SHR aorta. Influence of age and antihypertensive treatment.", "content": "Isometric contractions induced by noradrenaline (NA), 1.8 X 10(-5) M or by potassium (K+), 127 mM were studied in paired ring-preparations of the thoracic aortae from spontaneously hypertensive rats (SHR) and age-matched normotensive Kyoto-Wistar rats. In rats aged 8--16 weeks, NA-induced contractions were significantly more dependent on extracellular calcium in preparations from the SHR than from the NWR, whereas K+induced contractions showed no difference. Relaxation studies revealed differences between SHR and NWR also in K+-induced contractions. Comparison of responses in NWRs aged 3--4 months and 10--12 months showed a significant increment in Ca++-dependency with age. This age-related difference was less pronounced in SHRs, but the effect of blockade of Ca++-influx by nifedipine was significantly stronger in the old than in the young SHR-aorta. Treatment with propranolol or hydrochlorothiazide + timolol + minoxidil for 4--5 months caused no significant reduction of blood pressure and no change in Ca++-dependency. In contrast, treatment with verapamil (60 mg/kg/day) for 12 months resulted in a significantly lower blood pressure in the treated SHRs than in their controls. A trend towards \"nomrlization\" of the Ca++-dependency in verapamil treated rats was also observed. The results suggest that an increased Ca++-dependency in the SHR aortae is present already at the age of 8--16 weeks, but becomes more pronounced with age. As an age-related increment in Ca++-dependency is also found in NWRs, the results suggest that the SHR aortae are \"functionally\" older than the NWR vessels already in young animals. Calcium antagonists seem to be effective in lowering blood pressure in SHRs and represent a promising approach to the treatment of hypertensive vascular disease.", "contents": "Role of extracellular calcium in isometric contractions of the SHR aorta. Influence of age and antihypertensive treatment. Isometric contractions induced by noradrenaline (NA), 1.8 X 10(-5) M or by potassium (K+), 127 mM were studied in paired ring-preparations of the thoracic aortae from spontaneously hypertensive rats (SHR) and age-matched normotensive Kyoto-Wistar rats. In rats aged 8--16 weeks, NA-induced contractions were significantly more dependent on extracellular calcium in preparations from the SHR than from the NWR, whereas K+induced contractions showed no difference. Relaxation studies revealed differences between SHR and NWR also in K+-induced contractions. Comparison of responses in NWRs aged 3--4 months and 10--12 months showed a significant increment in Ca++-dependency with age. This age-related difference was less pronounced in SHRs, but the effect of blockade of Ca++-influx by nifedipine was significantly stronger in the old than in the young SHR-aorta. Treatment with propranolol or hydrochlorothiazide + timolol + minoxidil for 4--5 months caused no significant reduction of blood pressure and no change in Ca++-dependency. In contrast, treatment with verapamil (60 mg/kg/day) for 12 months resulted in a significantly lower blood pressure in the treated SHRs than in their controls. A trend towards \"nomrlization\" of the Ca++-dependency in verapamil treated rats was also observed. The results suggest that an increased Ca++-dependency in the SHR aortae is present already at the age of 8--16 weeks, but becomes more pronounced with age. As an age-related increment in Ca++-dependency is also found in NWRs, the results suggest that the SHR aortae are \"functionally\" older than the NWR vessels already in young animals. Calcium antagonists seem to be effective in lowering blood pressure in SHRs and represent a promising approach to the treatment of hypertensive vascular disease."}
{"id": "PMID:485726", "title": "Effects of intracerebroventricularly injected \"nerveside\" on free behaviour and EEG activity of rats. A pilot study.", "content": "\"Nerveside\", a phosphopeptide extracted from ox and dog brains by Toh, was injected into one lateral ventricle of four rats previously prepared for the injection as well as for recordings of the EEG of the dorsal hippocampus (field CA1) and sensorimotor cortex. \"Nerveside\" in doses of 150 and 300 micrograms/animal induced a transient stereotyped behaviour and longer lasting alterations of the EEG activity and vigilance pattern. Wakefulness was increased, slow wave sleep and REM-sleep were diminished. The vigilance pattern was characterized by rapid shifts between all three vigilance states. The effects of \"nerveside\" were visible for about three hours.", "contents": "Effects of intracerebroventricularly injected \"nerveside\" on free behaviour and EEG activity of rats. A pilot study. \"Nerveside\", a phosphopeptide extracted from ox and dog brains by Toh, was injected into one lateral ventricle of four rats previously prepared for the injection as well as for recordings of the EEG of the dorsal hippocampus (field CA1) and sensorimotor cortex. \"Nerveside\" in doses of 150 and 300 micrograms/animal induced a transient stereotyped behaviour and longer lasting alterations of the EEG activity and vigilance pattern. Wakefulness was increased, slow wave sleep and REM-sleep were diminished. The vigilance pattern was characterized by rapid shifts between all three vigilance states. The effects of \"nerveside\" were visible for about three hours."}
{"id": "PMID:485727", "title": "On the cardiac efficacy of helveticosol-3',4'-di-nitrate, a new glycoside.", "content": "The cardiac and extracardiac effects of a new glycoside, helveticosol-3',4'-di-nitrate (HdN), were compared with those of helveticosol (H) and helveticosol-3',4'-di-propionate (HdP) in isolated guinea-pig heart preparations, in volunteers and dogs. In the atrium and heart, HdN produces positive inotropic effects at the same concentrations as H and at 10 times lower concentrations than HdP. In the heart, the difference between equieffective concentrations of HdN and HdP is smaller, but this is true only for the concentration required to produce the maximum inotropic effect. In the anaesthetized dog equieffective cardiac effects are obtained after oral admiinistration of the glycosides in the ratio 1 : 2 : 0.5 (HdN : HdP : H). After administration of HdN and HdP at equal ratios, no significant differences were observed between the toxic effects in conscious dogs or in the extent of shortening of STI in volunteers. Replacement of the hydrophobic subsituent propionate for the more hydrophilic nitrate residue causes slight changes in cardiac efficiency.", "contents": "On the cardiac efficacy of helveticosol-3',4'-di-nitrate, a new glycoside. The cardiac and extracardiac effects of a new glycoside, helveticosol-3',4'-di-nitrate (HdN), were compared with those of helveticosol (H) and helveticosol-3',4'-di-propionate (HdP) in isolated guinea-pig heart preparations, in volunteers and dogs. In the atrium and heart, HdN produces positive inotropic effects at the same concentrations as H and at 10 times lower concentrations than HdP. In the heart, the difference between equieffective concentrations of HdN and HdP is smaller, but this is true only for the concentration required to produce the maximum inotropic effect. In the anaesthetized dog equieffective cardiac effects are obtained after oral admiinistration of the glycosides in the ratio 1 : 2 : 0.5 (HdN : HdP : H). After administration of HdN and HdP at equal ratios, no significant differences were observed between the toxic effects in conscious dogs or in the extent of shortening of STI in volunteers. Replacement of the hydrophobic subsituent propionate for the more hydrophilic nitrate residue causes slight changes in cardiac efficiency."}
{"id": "PMID:485728", "title": "Studies on the inactivation of vasoactive polypeptides by pyridoxal-5'-phosphate.", "content": "The pharmacologically active peptides bradykinin (BK) lysylbradykinin or kallidin (LBK) and methionyl-lysylbradykinin (MLBK) were shown to react with pyridoxal-5'-phosphate (PLP) forming Schiff bases; upon reduction with sodium borohydride they were transformed into stable phosphopyridoxyl-kinins (PP-BK, PP-LBK and PP-MLBK). Amino acid analysis showed that the N-terminal alpha-aminogroup of the three kinins were efficiently blocked by phosphopyridoxyl groups: 98.8% for BK; 95.2% for LBK and 98.9% for MLBK. Guanidinium groups did not react with PLP. Estimated by guinea-pig ileum and the vascular permeability of rat skin assays respectively- the PP-kinins had the following average activities compared to the respective kinins: PP-BK, 0.20 and 0.16; PP-LBK, 0.05 and 0.17; PP-MLBK, 0.10 and 0.12. Isoleucyl5-angiotensin II and valyl5-angiotensin I were shown to be 50% inactivated (guinea-pig ileum assay) by reaction with PLP and reduction with borohydride.", "contents": "Studies on the inactivation of vasoactive polypeptides by pyridoxal-5'-phosphate. The pharmacologically active peptides bradykinin (BK) lysylbradykinin or kallidin (LBK) and methionyl-lysylbradykinin (MLBK) were shown to react with pyridoxal-5'-phosphate (PLP) forming Schiff bases; upon reduction with sodium borohydride they were transformed into stable phosphopyridoxyl-kinins (PP-BK, PP-LBK and PP-MLBK). Amino acid analysis showed that the N-terminal alpha-aminogroup of the three kinins were efficiently blocked by phosphopyridoxyl groups: 98.8% for BK; 95.2% for LBK and 98.9% for MLBK. Guanidinium groups did not react with PLP. Estimated by guinea-pig ileum and the vascular permeability of rat skin assays respectively- the PP-kinins had the following average activities compared to the respective kinins: PP-BK, 0.20 and 0.16; PP-LBK, 0.05 and 0.17; PP-MLBK, 0.10 and 0.12. Isoleucyl5-angiotensin II and valyl5-angiotensin I were shown to be 50% inactivated (guinea-pig ileum assay) by reaction with PLP and reduction with borohydride."}
{"id": "PMID:485729", "title": "The role of endogenous prostaglandins in the renal vascular response to clonidine.", "content": "The effect of clonidine has been studied in the isolated perfused rabbit kidney. The drug produced a dose-dependent increase in perfusion pressure and in urine flow both of which could be blocked by phentolamine. Addition of acetylsalicylic acid to the medium caused an increase in perfusion pressure and a decrease in urine volume induced by clonidine. Further addition of prostaglandin E2 to the medium prevented the changes induced by acetylsalicylic acid. These results were taken as evidence that clonidine can increase the biosynthesis of prostaglandins which could act as a modulator system for the effects of the drug in the kidney.", "contents": "The role of endogenous prostaglandins in the renal vascular response to clonidine. The effect of clonidine has been studied in the isolated perfused rabbit kidney. The drug produced a dose-dependent increase in perfusion pressure and in urine flow both of which could be blocked by phentolamine. Addition of acetylsalicylic acid to the medium caused an increase in perfusion pressure and a decrease in urine volume induced by clonidine. Further addition of prostaglandin E2 to the medium prevented the changes induced by acetylsalicylic acid. These results were taken as evidence that clonidine can increase the biosynthesis of prostaglandins which could act as a modulator system for the effects of the drug in the kidney."}
{"id": "PMID:485730", "title": "Failure of phenoxybenzamine and pimozide to diminish changes in oxygen consumption and body temperature produced by caffeine.", "content": "The effects of a dopamine antagonist (pimozide) and an alpha-adrenergic receptor blocking agent (phenoxybenzamine) on caffeine-induced changes in oxygen consumption. body temperature, blood glucose and non-esterified fatty acids (NEFA) were studied in mice. Both drugs had no effect on the increase of the oxygen consumption produced by caffeine. The decline of the body temperature induced by caffeine was not significantly influenced by pimozide, while phenoxybenzamine accentuated the hypothermic effect of caffeine. The results indicate that alpha-adrenergic and dopamine receptors do not play an essential role in the caffeine-induced stimulation of overall metabolism and hypothermia.", "contents": "Failure of phenoxybenzamine and pimozide to diminish changes in oxygen consumption and body temperature produced by caffeine. The effects of a dopamine antagonist (pimozide) and an alpha-adrenergic receptor blocking agent (phenoxybenzamine) on caffeine-induced changes in oxygen consumption. body temperature, blood glucose and non-esterified fatty acids (NEFA) were studied in mice. Both drugs had no effect on the increase of the oxygen consumption produced by caffeine. The decline of the body temperature induced by caffeine was not significantly influenced by pimozide, while phenoxybenzamine accentuated the hypothermic effect of caffeine. The results indicate that alpha-adrenergic and dopamine receptors do not play an essential role in the caffeine-induced stimulation of overall metabolism and hypothermia."}
{"id": "PMID:485731", "title": "A study of the effect of vinca alkaloid 'vinblastine' on gastrointestinal motility in rats.", "content": "Vinblastine an alkaloid of the periwinkleplant (Vinca), produced marked alterations in the motility of gastrointestinal tract in rats. The alkaloid reduced the rate of gastric emptying and slowed the transit of test substance through the small intestine. The effect of the drug on gastric emptying appeared to be dose-dependent. The latency of drug action on gut motility was found to be 5--10 min, when injected through the jugular vein.", "contents": "A study of the effect of vinca alkaloid 'vinblastine' on gastrointestinal motility in rats. Vinblastine an alkaloid of the periwinkleplant (Vinca), produced marked alterations in the motility of gastrointestinal tract in rats. The alkaloid reduced the rate of gastric emptying and slowed the transit of test substance through the small intestine. The effect of the drug on gastric emptying appeared to be dose-dependent. The latency of drug action on gut motility was found to be 5--10 min, when injected through the jugular vein."}
{"id": "PMID:485732", "title": "Chromatographic fractionation and characterization of the active platelet aggregation inhibitory factor from bromelain.", "content": "Isolation and characterization of the platelet aggregation inhibitory factor of bromelain have been presented in this study. Commercial bromelain consists of 3 major components as demonstrated by discontinuous sodium chloride gradient chromatography through carbixymethyl-sephadex column. Fraction I constituted approximately 19% of the total fraction. This fraction had no proteolytic activity or platelet aggregation inhibiting activity, but showed peroxidatic activity. Fraction II and III, which constituted the remainder of the fraction eluted with 135 mM and 800 mM NaCl concentrations, respectively, showed both proteolytic and inhibition of platelet aggregation, but no peroxidatic activity. Immunoelectrophoresis and polyacrylamide electrophoresis showed fraction I with beta-mobility while fraction II and III demonstrated gamma-mobility. It is suggested that the proteolytic activity is associated with the inhibition of platelet aggregation, since oxidation of fractions II and III with sodium tetrathionate abolished both activities. The mechanism of inhibition of platelet aggregation by bromelain is presently unknown but may involve its influence on the prostaglandin synthetic pathway of platelets.", "contents": "Chromatographic fractionation and characterization of the active platelet aggregation inhibitory factor from bromelain. Isolation and characterization of the platelet aggregation inhibitory factor of bromelain have been presented in this study. Commercial bromelain consists of 3 major components as demonstrated by discontinuous sodium chloride gradient chromatography through carbixymethyl-sephadex column. Fraction I constituted approximately 19% of the total fraction. This fraction had no proteolytic activity or platelet aggregation inhibiting activity, but showed peroxidatic activity. Fraction II and III, which constituted the remainder of the fraction eluted with 135 mM and 800 mM NaCl concentrations, respectively, showed both proteolytic and inhibition of platelet aggregation, but no peroxidatic activity. Immunoelectrophoresis and polyacrylamide electrophoresis showed fraction I with beta-mobility while fraction II and III demonstrated gamma-mobility. It is suggested that the proteolytic activity is associated with the inhibition of platelet aggregation, since oxidation of fractions II and III with sodium tetrathionate abolished both activities. The mechanism of inhibition of platelet aggregation by bromelain is presently unknown but may involve its influence on the prostaglandin synthetic pathway of platelets."}
{"id": "PMID:485740", "title": "Serum electrolyte and acid base composition. The influence of graded degrees of chronic renal failure.", "content": "Data from 41 ambulatory patients with graded degrees of uncomplicated, chronic renal failure were used to define the quantitative relationship between serum acid-base and electrolyte composition and the serum creatinine level. Even in patients with only moderate renal insufficiency, serum total carbon dioxide (tCO2) content was reduced significantly. This early fall in tCO2 was offset by an increase in serum chloride (Cl-), serum undetermined anton concentration (A-) remaining normal. In patients with more severe degrees of renal insufficiency, further decrements in tCO2 occurred that were proportional to the increment in serum creatinine. These latter decrements in tCO2 were associated with equivalent increments in A-, serum Cl- remaining unchanged at the elevated level observed during moderate renal insufficiency. Confidence limits of 95% for tCO2 and A- were calculated from the data.", "contents": "Serum electrolyte and acid base composition. The influence of graded degrees of chronic renal failure. Data from 41 ambulatory patients with graded degrees of uncomplicated, chronic renal failure were used to define the quantitative relationship between serum acid-base and electrolyte composition and the serum creatinine level. Even in patients with only moderate renal insufficiency, serum total carbon dioxide (tCO2) content was reduced significantly. This early fall in tCO2 was offset by an increase in serum chloride (Cl-), serum undetermined anton concentration (A-) remaining normal. In patients with more severe degrees of renal insufficiency, further decrements in tCO2 occurred that were proportional to the increment in serum creatinine. These latter decrements in tCO2 were associated with equivalent increments in A-, serum Cl- remaining unchanged at the elevated level observed during moderate renal insufficiency. Confidence limits of 95% for tCO2 and A- were calculated from the data."}
{"id": "PMID:485741", "title": "Leukopenia and hypoxemia. Unrelated effects of hemodialysis.", "content": "Hemodialysis-induced hypoxemia has been attributed to membrane-related complement activation leading to pulmonary leukostasis and to hypoventilation secondary to carbon dioxide losses via the dialyzer. We have separately assessed the role of membrane- and dialysis-related factors by using different dialyzers and sequential ultrafiltration and hemodialysis with first-use cellulose dialyzers produced both leukopenia and hypoxemia. With reused cellulose and polyacrylonitrile dialyzers, hypoxemia still occurred, but without leukopenia. Ultrafiltration produced leukopenia and no changes in Pao2; during the subsequent hemodialysis, hypoxemia developed as the leukocyte count increased by 50%. Our data indicate that leukopenia and hypoxemia are unrelated effects of hemodialysis, and favor hypoventilation as the major determinant of hypoxemia during hemodialysis.", "contents": "Leukopenia and hypoxemia. Unrelated effects of hemodialysis. Hemodialysis-induced hypoxemia has been attributed to membrane-related complement activation leading to pulmonary leukostasis and to hypoventilation secondary to carbon dioxide losses via the dialyzer. We have separately assessed the role of membrane- and dialysis-related factors by using different dialyzers and sequential ultrafiltration and hemodialysis with first-use cellulose dialyzers produced both leukopenia and hypoxemia. With reused cellulose and polyacrylonitrile dialyzers, hypoxemia still occurred, but without leukopenia. Ultrafiltration produced leukopenia and no changes in Pao2; during the subsequent hemodialysis, hypoxemia developed as the leukocyte count increased by 50%. Our data indicate that leukopenia and hypoxemia are unrelated effects of hemodialysis, and favor hypoventilation as the major determinant of hypoxemia during hemodialysis."}
{"id": "PMID:485742", "title": "Analysis of clinical susceptibility bias in case-control studies. Analysis as illustrated by the menopausal syndrome and the risk of endometrial cancer.", "content": "Epidemiologic studies of causes of disease rarely contain adjustments for inequalities in diseases susceptibility caused by baseline differences in clinical phenomena. In the controversial association between estrogens and endometrial cancer, the menopausal syndrome was suspected as an independent risk factor for the development of endometrial cancer, irrespective of estrogen use. To investigate this suspicion, personal interview data from a case-control investigation were collected and analyzed. The odds ratio for the association between menopausal symptoms and endometrial cancer was 1.12 and 0.85 for two different sets of cases and controls assembled at the same institution. When the data were partitioned according to estrogen usage, the odds ratios became consistently less than one. The results suggest that the menopausal syndrome is not a risk factor for endometrial cancer.", "contents": "Analysis of clinical susceptibility bias in case-control studies. Analysis as illustrated by the menopausal syndrome and the risk of endometrial cancer. Epidemiologic studies of causes of disease rarely contain adjustments for inequalities in diseases susceptibility caused by baseline differences in clinical phenomena. In the controversial association between estrogens and endometrial cancer, the menopausal syndrome was suspected as an independent risk factor for the development of endometrial cancer, irrespective of estrogen use. To investigate this suspicion, personal interview data from a case-control investigation were collected and analyzed. The odds ratio for the association between menopausal symptoms and endometrial cancer was 1.12 and 0.85 for two different sets of cases and controls assembled at the same institution. When the data were partitioned according to estrogen usage, the odds ratios became consistently less than one. The results suggest that the menopausal syndrome is not a risk factor for endometrial cancer."}
{"id": "PMID:485743", "title": "Serum thyroid hormone levels in patients with liver disease.", "content": "Levels of serum triiodothyronine (T3), reverse triiodothyronine (rT3), and thyroxine (T4) were determined in 29 patients with alcoholic cirrhosis, seven patients with acute hepatitis, and 14 control patients hospitalized for chronic disease. Serum T3 levels were decreased significantly and serum rT3 levels increased significantly in the patients with alcoholic cirrhosis. Serum T3 and T4 levels were lower and rT3 levels higher in the cirrhotic patients who died within three months of the study compared with those who survived. A combination of prothrombin time, aminopyrine breath test results, and rT3 and T3 determinations gave significant predictive information about survival in patients with cirrhosis. The data suggest that assay of serum thyroid hormone levels together with prothrombin time and the aminopyrine breath test may be helpful in assessing the course and prognosis of patients with liver disease.", "contents": "Serum thyroid hormone levels in patients with liver disease. Levels of serum triiodothyronine (T3), reverse triiodothyronine (rT3), and thyroxine (T4) were determined in 29 patients with alcoholic cirrhosis, seven patients with acute hepatitis, and 14 control patients hospitalized for chronic disease. Serum T3 levels were decreased significantly and serum rT3 levels increased significantly in the patients with alcoholic cirrhosis. Serum T3 and T4 levels were lower and rT3 levels higher in the cirrhotic patients who died within three months of the study compared with those who survived. A combination of prothrombin time, aminopyrine breath test results, and rT3 and T3 determinations gave significant predictive information about survival in patients with cirrhosis. The data suggest that assay of serum thyroid hormone levels together with prothrombin time and the aminopyrine breath test may be helpful in assessing the course and prognosis of patients with liver disease."}
{"id": "PMID:485744", "title": "Infectious arthritis.", "content": "One hundred thirteen patients with 120 episodes of septic arthritis were seen during a 14-year period. The most common bacteria cultured from joint fluid or blood during the acute episodes were gonococci, staphylococci, and streptococci. Seventeen other bacteria were the infecting organisms in one or more cases each. Other infections and medical conditions frequently were present. In some instances the septic arthritis was a complication of another infection. In other patients septic arthritis appeared to occur because of diminished resistance to infection. The majority of patients responded well to medical treatment, but eight died and 26 had persistence of articular pain at follow-up examination.", "contents": "Infectious arthritis. One hundred thirteen patients with 120 episodes of septic arthritis were seen during a 14-year period. The most common bacteria cultured from joint fluid or blood during the acute episodes were gonococci, staphylococci, and streptococci. Seventeen other bacteria were the infecting organisms in one or more cases each. Other infections and medical conditions frequently were present. In some instances the septic arthritis was a complication of another infection. In other patients septic arthritis appeared to occur because of diminished resistance to infection. The majority of patients responded well to medical treatment, but eight died and 26 had persistence of articular pain at follow-up examination."}
{"id": "PMID:485745", "title": "Solid tumors complicating Hodgkin's disease. A report on two patients with immunoglobulin deficiency.", "content": "Multiple epithelial malignant neoplasms developed in two patients with Hodgkin's disease subsequent to radiotherapy and intensive chemotherapy. At the time of diagnosis, each patient also demonstrated a serum immunoglobulin deficiency. The significance of the occurrence of solid tumors in patients following therapy for Hodgkin's disease and the significance of cellular and humoral immunodeficiency in Hodgkin's disease in relation to second cancer development were studied. We suggest the establishment of a registry of leukemias and solid tumors developing in patients treated for Hodgkin's disease and other malignant neoplasms, possibly with detailed recording of immunocompetence data.", "contents": "Solid tumors complicating Hodgkin's disease. A report on two patients with immunoglobulin deficiency. Multiple epithelial malignant neoplasms developed in two patients with Hodgkin's disease subsequent to radiotherapy and intensive chemotherapy. At the time of diagnosis, each patient also demonstrated a serum immunoglobulin deficiency. The significance of the occurrence of solid tumors in patients following therapy for Hodgkin's disease and the significance of cellular and humoral immunodeficiency in Hodgkin's disease in relation to second cancer development were studied. We suggest the establishment of a registry of leukemias and solid tumors developing in patients treated for Hodgkin's disease and other malignant neoplasms, possibly with detailed recording of immunocompetence data."}
{"id": "PMID:485746", "title": "Retinal arteriolar changes as an indicator of coronary artery disease.", "content": "Funduscopic examination was performed in 70 non diabetic, nonhypertensive patients without valvular heart disease undergoing coronary angiography for evaluation of chest pain syndromes to determine if retinal arteriolar changes could reliably predict presence of coronary artery disease. Retinal arteriolar changes were graded with respect to light reflex, vessel caliber, arteriovenous crossing defects, and vessel tortuosity without knowledge of angiographic findings. Each coronary vessel was graded with respect to its most occlusive lesion by angiography; coronary index was derived for each patient without knowledge of eye findings. Abnormal light reflex changes were the most sensitive indicators of presence and extent of coronary artery disease. Abnormal vessel tortuosity and decreased caliber were less sensitive but more specific; their presence also suggested more extensive coronary lesions. Thus, funduscopic examination demonstrating specific retinal arteriolar lesions may indicate presence of coronary artery disease and may correlate with extent of lesions in selected patients.", "contents": "Retinal arteriolar changes as an indicator of coronary artery disease. Funduscopic examination was performed in 70 non diabetic, nonhypertensive patients without valvular heart disease undergoing coronary angiography for evaluation of chest pain syndromes to determine if retinal arteriolar changes could reliably predict presence of coronary artery disease. Retinal arteriolar changes were graded with respect to light reflex, vessel caliber, arteriovenous crossing defects, and vessel tortuosity without knowledge of angiographic findings. Each coronary vessel was graded with respect to its most occlusive lesion by angiography; coronary index was derived for each patient without knowledge of eye findings. Abnormal light reflex changes were the most sensitive indicators of presence and extent of coronary artery disease. Abnormal vessel tortuosity and decreased caliber were less sensitive but more specific; their presence also suggested more extensive coronary lesions. Thus, funduscopic examination demonstrating specific retinal arteriolar lesions may indicate presence of coronary artery disease and may correlate with extent of lesions in selected patients."}
{"id": "PMID:485747", "title": "Therapy of primary amyloidosis with melphalan and prednisone.", "content": "Two patients with progressive primary amyloidosis, monoclonal serum and urinary proteins, multiple organ involvement, and nephrotic syndrome were treated with melphalan and prednisone for one year. In one patient, splenomegaly and nephrotic syndrome rapidly responded to therapy but massive hepatomegaly responded slowly, requiring 15 months' time for normalization of size. Results of liver function tests, although improved, remained abnormal, and amyloid deposits remained in the marrow. A second patient also demonstrated dramatic diminution in proteinuria and improvement in liver function abnormalities, but macroglossia persisted. These observations suggest that amyloid organ involvement may be reversible with differences in organ responsiveness to chemotherapy. An empirical trial of chemotherapy may be indicated in some patients with progressive primary amyloidosis, and therapy may need to be continued for a prolonged period of time before seeing an effect.", "contents": "Therapy of primary amyloidosis with melphalan and prednisone. Two patients with progressive primary amyloidosis, monoclonal serum and urinary proteins, multiple organ involvement, and nephrotic syndrome were treated with melphalan and prednisone for one year. In one patient, splenomegaly and nephrotic syndrome rapidly responded to therapy but massive hepatomegaly responded slowly, requiring 15 months' time for normalization of size. Results of liver function tests, although improved, remained abnormal, and amyloid deposits remained in the marrow. A second patient also demonstrated dramatic diminution in proteinuria and improvement in liver function abnormalities, but macroglossia persisted. These observations suggest that amyloid organ involvement may be reversible with differences in organ responsiveness to chemotherapy. An empirical trial of chemotherapy may be indicated in some patients with progressive primary amyloidosis, and therapy may need to be continued for a prolonged period of time before seeing an effect."}
{"id": "PMID:485748", "title": "Altered control of growth hormone secretion in patients with cirrhosis of the liver.", "content": "Ten male patients with cirrhosis of the liver (three with portacaval anastomosis [PCA]) and eight sex- and age-matched controls underwent an arginine infusion test followed by an intravenous glucose tolerance test. Plasma glucose and growth hormone (GH) levels were measured during a period of three hours. In the normal subjects, the peak GH response to arginine occurred 60 minutes after the start of the infusion and was followed by a progressive decline in GH concentration; dextrose injection resulted in a further rapid fall in GH concentration. In cirrhotic patients, both fasting and postarginine GH concentrations were significantly higher than in controls; in addition, the dextrose injection, after causing a transitory drop in plasma GH levels, resulted in a marked increase in plasma GH concentration. In the patients with PCA, the plasma GH increase after arginine and after dextrous was more marked. In these cirrhotic patients, the plasma GH levels correlated directly with the magnitude of the portal hypertension and inversely with the serum albumin concentration, suggesting that the abnormality of GH secretion was a reflection of the derangement in liver function.", "contents": "Altered control of growth hormone secretion in patients with cirrhosis of the liver. Ten male patients with cirrhosis of the liver (three with portacaval anastomosis [PCA]) and eight sex- and age-matched controls underwent an arginine infusion test followed by an intravenous glucose tolerance test. Plasma glucose and growth hormone (GH) levels were measured during a period of three hours. In the normal subjects, the peak GH response to arginine occurred 60 minutes after the start of the infusion and was followed by a progressive decline in GH concentration; dextrose injection resulted in a further rapid fall in GH concentration. In cirrhotic patients, both fasting and postarginine GH concentrations were significantly higher than in controls; in addition, the dextrose injection, after causing a transitory drop in plasma GH levels, resulted in a marked increase in plasma GH concentration. In the patients with PCA, the plasma GH increase after arginine and after dextrous was more marked. In these cirrhotic patients, the plasma GH levels correlated directly with the magnitude of the portal hypertension and inversely with the serum albumin concentration, suggesting that the abnormality of GH secretion was a reflection of the derangement in liver function."}
{"id": "PMID:485749", "title": "Cardiac findings in Charcot-Marie-Tooth disease. A prospective study of 68 patients.", "content": "Charcot-Marie-Tooth disease (peroneal muscular atrophy) has been reported to cause cardiac arrthymias and conduction disturbances in association with peripheral muscle atrophy. To establish more accurately the frequency of such cardiac disorders in this disease, 68 patients with Charcot-Marie-Tooth disease were evaluated prospectively for evidence of cardiac involvement. Cardiac findings were limited to five patients with conduction defects, two patients with supraventricular tachycardia, two patients with ischemic heart disease, and 20 with mitral valve prolapse. The frequency of each of the abnormal cardiac findings, with the possibly emalities in the population at large. The low incidence of cardiac involvement in patients with Charcot-Marie-Tooth disease may be helpful in distinguishing this disorder from Friedreich's ataxia, an entity that may mimic Charcot-Marie-Tooth disease but that is frequently associated with heart disease.", "contents": "Cardiac findings in Charcot-Marie-Tooth disease. A prospective study of 68 patients. Charcot-Marie-Tooth disease (peroneal muscular atrophy) has been reported to cause cardiac arrthymias and conduction disturbances in association with peripheral muscle atrophy. To establish more accurately the frequency of such cardiac disorders in this disease, 68 patients with Charcot-Marie-Tooth disease were evaluated prospectively for evidence of cardiac involvement. Cardiac findings were limited to five patients with conduction defects, two patients with supraventricular tachycardia, two patients with ischemic heart disease, and 20 with mitral valve prolapse. The frequency of each of the abnormal cardiac findings, with the possibly emalities in the population at large. The low incidence of cardiac involvement in patients with Charcot-Marie-Tooth disease may be helpful in distinguishing this disorder from Friedreich's ataxia, an entity that may mimic Charcot-Marie-Tooth disease but that is frequently associated with heart disease."}
{"id": "PMID:485750", "title": "Development of a research program in a community teaching hospital.", "content": "Affiliation between community hospitals and university centers has become a prominent trend. St Mary's Hospital, a 298-bed community hospital, affiliated with the University of Rochester, School of Medicine and Dentistry, and in five years developed an academic program. The strategies we utilized included the following: (1) definition of the number of specialists needed for the hospital's patient population; (2) development of specific subspecialty units with more than one member with the primary intent of fostering an investigative program; (3) analysis of total projected faculty salary and laboratory renovation and equipment costs to allow staged development of the program; (4) design of a flexible research areas; and (5) joint use of equipment and space with the Clinical Laboratory Service. The program was financed through third-party reimbursement, faculty-generated Department of Medicine funds, gifts by private patrons, and extramural grants. At a time of diminishing investigative support, a medium-sized community hospital can develop a research program.", "contents": "Development of a research program in a community teaching hospital. Affiliation between community hospitals and university centers has become a prominent trend. St Mary's Hospital, a 298-bed community hospital, affiliated with the University of Rochester, School of Medicine and Dentistry, and in five years developed an academic program. The strategies we utilized included the following: (1) definition of the number of specialists needed for the hospital's patient population; (2) development of specific subspecialty units with more than one member with the primary intent of fostering an investigative program; (3) analysis of total projected faculty salary and laboratory renovation and equipment costs to allow staged development of the program; (4) design of a flexible research areas; and (5) joint use of equipment and space with the Clinical Laboratory Service. The program was financed through third-party reimbursement, faculty-generated Department of Medicine funds, gifts by private patrons, and extramural grants. At a time of diminishing investigative support, a medium-sized community hospital can develop a research program."}
{"id": "PMID:485751", "title": "Infected left atrial myxoma with bacteremia simulating infective endocarditis.", "content": "A 58-year-old man had intermittent fever of eight months' duration following a dental extraction. There were no abnormal cardiac auscultatory findings. Multiple blood cultures yielded Streptococcus mutans. Treatment for infective endocarditis was initiated; however, an echocardiogram suggested the presence of a left atrial myxoma. The diagnosis was confirmed by angiography and the infected tumor was removed successfully. Differentiating features between left atrial myxoma and mitral valve endocarditis may not be obvious clinically, and bacteremia does not preclude atrial myxoma as a diagnostic possibility. We therefore suggest that all cases of infective endocarditis be evaluated by echocardiography to elucidate lesions such as large vegetations or left atrial myxoma, both of which may require urgent operative intervention.", "contents": "Infected left atrial myxoma with bacteremia simulating infective endocarditis. A 58-year-old man had intermittent fever of eight months' duration following a dental extraction. There were no abnormal cardiac auscultatory findings. Multiple blood cultures yielded Streptococcus mutans. Treatment for infective endocarditis was initiated; however, an echocardiogram suggested the presence of a left atrial myxoma. The diagnosis was confirmed by angiography and the infected tumor was removed successfully. Differentiating features between left atrial myxoma and mitral valve endocarditis may not be obvious clinically, and bacteremia does not preclude atrial myxoma as a diagnostic possibility. We therefore suggest that all cases of infective endocarditis be evaluated by echocardiography to elucidate lesions such as large vegetations or left atrial myxoma, both of which may require urgent operative intervention."}
{"id": "PMID:485752", "title": "Pseudothrombophlebitis syndrome in the arthropathy of granulomatous colitis.", "content": "A case of pseudothrombophlebitis syndrome occurred in a young man with colitic arthropathy who was seen because of a swollen leg. The salient features of this syndrome, the difficulty in differentiating it clinically from true deep venous thrombosis, and the importance of correct diagnosis are stressed, particularly in light of the fact that patients with inflammatory bowel disease are predisposed to occlusive venous disease.", "contents": "Pseudothrombophlebitis syndrome in the arthropathy of granulomatous colitis. A case of pseudothrombophlebitis syndrome occurred in a young man with colitic arthropathy who was seen because of a swollen leg. The salient features of this syndrome, the difficulty in differentiating it clinically from true deep venous thrombosis, and the importance of correct diagnosis are stressed, particularly in light of the fact that patients with inflammatory bowel disease are predisposed to occlusive venous disease."}
{"id": "PMID:485753", "title": "The echocardiogram in scleroderma endocarditis of the mitral valve.", "content": "Echocardiography was performed in a woman with long-standing scleroderma with the CRST syndrome in whom the murmur of mitral regurgitation had been present for one year. The echocardiogram showed an irregular shaggy thickening of the anterior mitral valve leaflet. At autopsy three months later, myocardial fibrosis, small coronary artery sclerosis, and nodular thickening of the mitral valve were found. To our knowledge, anterior mitral leaflet thickening in scleroderma has not been reported previously. In this patient, it was associated with a poor prognosis.", "contents": "The echocardiogram in scleroderma endocarditis of the mitral valve. Echocardiography was performed in a woman with long-standing scleroderma with the CRST syndrome in whom the murmur of mitral regurgitation had been present for one year. The echocardiogram showed an irregular shaggy thickening of the anterior mitral valve leaflet. At autopsy three months later, myocardial fibrosis, small coronary artery sclerosis, and nodular thickening of the mitral valve were found. To our knowledge, anterior mitral leaflet thickening in scleroderma has not been reported previously. In this patient, it was associated with a poor prognosis."}
{"id": "PMID:485754", "title": "Agranulocytosis associated with aprindine therapy.", "content": "Aprindine hydrochloride is an antiarrhythmic drug now under investigation. The drug is most effective in prophylaxis of ventricular dysrhythmias. Previously recognized toxic effects include neurological side effects, nausea, vomiting, as well as a reported association with agranulocytosis. To our knowledge, this is the first report of agranulocytosis associated with documented elevated serum concentrations of aprindine. Further correlation of drug levels with toxic reactions may help in establishing the mechanism of bone marrow suppression.", "contents": "Agranulocytosis associated with aprindine therapy. Aprindine hydrochloride is an antiarrhythmic drug now under investigation. The drug is most effective in prophylaxis of ventricular dysrhythmias. Previously recognized toxic effects include neurological side effects, nausea, vomiting, as well as a reported association with agranulocytosis. To our knowledge, this is the first report of agranulocytosis associated with documented elevated serum concentrations of aprindine. Further correlation of drug levels with toxic reactions may help in establishing the mechanism of bone marrow suppression."}
{"id": "PMID:485755", "title": "Hematogenous vertebral osteomyelitis. Pasteurella multocida as the causative agent.", "content": "Human disease caused by Pasteurella multocida is well documented, and a wide variety of clinical syndromes have been described. We describe herein a patient with vertebral osteomyelitis, discitis, and paravertebral abscess caused by P multocida, a presentation that has not been described previously, to our knowledge.", "contents": "Hematogenous vertebral osteomyelitis. Pasteurella multocida as the causative agent. Human disease caused by Pasteurella multocida is well documented, and a wide variety of clinical syndromes have been described. We describe herein a patient with vertebral osteomyelitis, discitis, and paravertebral abscess caused by P multocida, a presentation that has not been described previously, to our knowledge."}
{"id": "PMID:485756", "title": "Membranous nephropathy with epithelial crescents in a patient with pulmonary sarcoidosis.", "content": "Renal failure in patients with pulmonary or extrapulmonary sarcoidosis has been attributed to interstitial disease. Reports of cases of primary glomerular abnormality with renal failure in patients with sarcoidosis are rare. We describe a patient with pulmonary sarcoidosis and renal failure due to membranous nephropathy with epithelial crescents. A review of primary glomerular involvement in patients with sarcoidosis and the association of immune complexes in the pathogenesis of the two diseases is discussed.", "contents": "Membranous nephropathy with epithelial crescents in a patient with pulmonary sarcoidosis. Renal failure in patients with pulmonary or extrapulmonary sarcoidosis has been attributed to interstitial disease. Reports of cases of primary glomerular abnormality with renal failure in patients with sarcoidosis are rare. We describe a patient with pulmonary sarcoidosis and renal failure due to membranous nephropathy with epithelial crescents. A review of primary glomerular involvement in patients with sarcoidosis and the association of immune complexes in the pathogenesis of the two diseases is discussed."}
{"id": "PMID:485757", "title": "Hypouricemia and malignant neoplasms. A new case of xanthinuria.", "content": "An 80-year-old man with squamous cell carcinoma of the parotid gland was noted to have persistent severe hypouricemia with serum uric acid values of 0.5 to 1.0 mg/dL. Urinary excretion of uric acid was very low and a diagnosis of xanthinuria was made.", "contents": "Hypouricemia and malignant neoplasms. A new case of xanthinuria. An 80-year-old man with squamous cell carcinoma of the parotid gland was noted to have persistent severe hypouricemia with serum uric acid values of 0.5 to 1.0 mg/dL. Urinary excretion of uric acid was very low and a diagnosis of xanthinuria was made."}
{"id": "PMID:485758", "title": "Idiopathic hypothalamic hypogonadotropic hypogonadism with polyostotic fibrous dysplasia.", "content": "A 22-year-old woman had polyostotic fibrous dysplasia (POFD) and idiopathic hypothalamic hypogonadotropic hypogonadism (isolated gonadotropin deficiency). Recurrent fracture of dysplastic bone during childhood was associated with primary amenorrhea, clinical and laboratory evidence of estrogen deficiency, and subnormal circulating and urinary gonadotropin levels during adolescence. Gonadorelin (luteinizing hormone-releasing hormone) stimulation initially showed a luteinizing hormone (LH) response but absent follicle-stimulating hormone (FSH) response. After three months without estrogen and progesterone and after four days of gonadorelin \"priming,\" a subsequent gonadorelin infusion produced an enhanced LH and FSH response. All other tests of peripheral and trophic hormone levels and pituitary trophic hormone reserves were normal. Whereas POFD is known to occur with sexual precocity and other endocrinopathies, to our knowledge this is the first report of its association with isolated gonadotropin deficiency.", "contents": "Idiopathic hypothalamic hypogonadotropic hypogonadism with polyostotic fibrous dysplasia. A 22-year-old woman had polyostotic fibrous dysplasia (POFD) and idiopathic hypothalamic hypogonadotropic hypogonadism (isolated gonadotropin deficiency). Recurrent fracture of dysplastic bone during childhood was associated with primary amenorrhea, clinical and laboratory evidence of estrogen deficiency, and subnormal circulating and urinary gonadotropin levels during adolescence. Gonadorelin (luteinizing hormone-releasing hormone) stimulation initially showed a luteinizing hormone (LH) response but absent follicle-stimulating hormone (FSH) response. After three months without estrogen and progesterone and after four days of gonadorelin \"priming,\" a subsequent gonadorelin infusion produced an enhanced LH and FSH response. All other tests of peripheral and trophic hormone levels and pituitary trophic hormone reserves were normal. Whereas POFD is known to occur with sexual precocity and other endocrinopathies, to our knowledge this is the first report of its association with isolated gonadotropin deficiency."}
{"id": "PMID:485759", "title": "Runaway pacemaker. Unpredictable pacemaker failure.", "content": "The runaway pacemaker is an uncommon but very serious complication of permanent artificial pacemakers. Although the runaway pacemaker has been most frequently encountered in older (fixed rate) pacemakers, this problem has also been reported in various types of newer models manufactured by different companies. The most striking finding in our case is that the pacemaker was reported by a reliable pacemaker follow-up service to be working normally only one week before the development of the malfunction. The extremely rapid pacing rate (750 beats per minute) was a manifestation of a far-advanced runaway pacemaker. The manufacturer believes that this is the first reported incident of a runaway pacemaker in this model. This indicates that the runaway pacemaker is still a potential problem, even in newer pacemakers, and reemphasizes the unpredictable and serious nature of this medical emergency.", "contents": "Runaway pacemaker. Unpredictable pacemaker failure. The runaway pacemaker is an uncommon but very serious complication of permanent artificial pacemakers. Although the runaway pacemaker has been most frequently encountered in older (fixed rate) pacemakers, this problem has also been reported in various types of newer models manufactured by different companies. The most striking finding in our case is that the pacemaker was reported by a reliable pacemaker follow-up service to be working normally only one week before the development of the malfunction. The extremely rapid pacing rate (750 beats per minute) was a manifestation of a far-advanced runaway pacemaker. The manufacturer believes that this is the first reported incident of a runaway pacemaker in this model. This indicates that the runaway pacemaker is still a potential problem, even in newer pacemakers, and reemphasizes the unpredictable and serious nature of this medical emergency."}
{"id": "PMID:485760", "title": "Bacterial endocarditis in a patient with mitral valve prolapse.", "content": "Bacterial endocarditis developed following dental manipulation in a child with mitral valve prolapse. Antibiotic prophylaxis is recommended for patients with this cardiac abnormality who undergo procedures that may be associated with bacteremia.", "contents": "Bacterial endocarditis in a patient with mitral valve prolapse. Bacterial endocarditis developed following dental manipulation in a child with mitral valve prolapse. Antibiotic prophylaxis is recommended for patients with this cardiac abnormality who undergo procedures that may be associated with bacteremia."}
{"id": "PMID:485765", "title": "[Metabolic products of microorganisms. 175. Tetracenomycin C (author's transl)].", "content": "Streptomyces glaucescens, strain T\u00fc 49 = ETH 22794, produces hydroxystreptomycin as well as the tetracenomycins, a mixture of several lipophilic antibiotics. The main component and the most active one is tetracenomycin C. Tetracenomycin C has a molecular formula C23H20O11 and is chemically related to tetracyclines and anthracyclinones. The pale yellow antibiotic is active against some gram-positive bacteria, especially against streptomycetes. Gram-negative bacteria and fungi are not inhibited. In considering the differences of biological activity and the functional groups of the molecule, tetracenomycin C is not a member of the tetracycline or anthracyclinone group of antibiotics.", "contents": "[Metabolic products of microorganisms. 175. Tetracenomycin C (author's transl)]. Streptomyces glaucescens, strain T\u00fc 49 = ETH 22794, produces hydroxystreptomycin as well as the tetracenomycins, a mixture of several lipophilic antibiotics. The main component and the most active one is tetracenomycin C. Tetracenomycin C has a molecular formula C23H20O11 and is chemically related to tetracyclines and anthracyclinones. The pale yellow antibiotic is active against some gram-positive bacteria, especially against streptomycetes. Gram-negative bacteria and fungi are not inhibited. In considering the differences of biological activity and the functional groups of the molecule, tetracenomycin C is not a member of the tetracycline or anthracyclinone group of antibiotics."}
{"id": "PMID:485767", "title": "[Mucolipidosis II (I cell disease): a sialidosis?].", "content": "I cell disease is characterised by loss of lysosomal enzymes from fibroblasts with accumulation of the same enzymes in the surrounding fluid. The urine oligosaccharides are composed by sialyl-oligosaccharides with equal numbers of alpha (2--6) and alpha (2--3) neuraminic acid -beta-galactoside bonds. A defect in the alpha (2--6) and alpha (2--3) neuraminidase activity in leucocytes and fibroblasts has been demonstrated. This deficit is the primary abnormality in I cell disease and it can be compared to the partial enzyme deficiencies described particularly in Mucolipidosis type I.", "contents": "[Mucolipidosis II (I cell disease): a sialidosis?]. I cell disease is characterised by loss of lysosomal enzymes from fibroblasts with accumulation of the same enzymes in the surrounding fluid. The urine oligosaccharides are composed by sialyl-oligosaccharides with equal numbers of alpha (2--6) and alpha (2--3) neuraminic acid -beta-galactoside bonds. A defect in the alpha (2--6) and alpha (2--3) neuraminidase activity in leucocytes and fibroblasts has been demonstrated. This deficit is the primary abnormality in I cell disease and it can be compared to the partial enzyme deficiencies described particularly in Mucolipidosis type I."}
{"id": "PMID:485768", "title": "[Hepatitis in 2 stages in children].", "content": "Twenty seven children with relapsing hepatitis have been reviewed. The characteristic clinical course and the excellent prognosis of this form of hepatitis is emphasised. After an apparently ordinary initial episode of hepatitis followed by recovery, a relapse occurs within 1--3 months that is usually more severe clinically and biochemically. The histological appearances are similar to those of acute hepatitis. A delayed but spontaneous recovery occurs. No satisfactory explanation has been proposed for this condition.", "contents": "[Hepatitis in 2 stages in children]. Twenty seven children with relapsing hepatitis have been reviewed. The characteristic clinical course and the excellent prognosis of this form of hepatitis is emphasised. After an apparently ordinary initial episode of hepatitis followed by recovery, a relapse occurs within 1--3 months that is usually more severe clinically and biochemically. The histological appearances are similar to those of acute hepatitis. A delayed but spontaneous recovery occurs. No satisfactory explanation has been proposed for this condition."}
{"id": "PMID:485769", "title": "[Neonatal hematemesis caused by peptic esophagitis].", "content": "Five full term neonates presented with haematemeses on one of the first three days of life. All but one had been given vitamin K at birth. Two out of five had clotting abnormalities that suggested vitamin K deficiency. All had evidence of hiatus hernia and, on oesophagoscopy, had evidence of peptic oesophagitis. Bleeding is another complication of neonatal peptic oesophagitis.", "contents": "[Neonatal hematemesis caused by peptic esophagitis]. Five full term neonates presented with haematemeses on one of the first three days of life. All but one had been given vitamin K at birth. Two out of five had clotting abnormalities that suggested vitamin K deficiency. All had evidence of hiatus hernia and, on oesophagoscopy, had evidence of peptic oesophagitis. Bleeding is another complication of neonatal peptic oesophagitis."}
{"id": "PMID:485770", "title": "[Familial tubulopathy associating hypercalciuria and saline diabetes. Favorable results of indomethacin therapy].", "content": "The association of hypercalciuria, salt losing renal disease, and a defect in urine concentrating ability with high renin and aldosterone levels is described in two brothers. One child had marked nephrocalcinosis by the age of 3 and the other severe growth retardation. In one child all the abnormalities were abolished with indomethacin which was responsible for a marked decrease in urinary. The data and the family study suggest that this condition is a proximal tubular disorder with atuosomal recessive inheritance.", "contents": "[Familial tubulopathy associating hypercalciuria and saline diabetes. Favorable results of indomethacin therapy]. The association of hypercalciuria, salt losing renal disease, and a defect in urine concentrating ability with high renin and aldosterone levels is described in two brothers. One child had marked nephrocalcinosis by the age of 3 and the other severe growth retardation. In one child all the abnormalities were abolished with indomethacin which was responsible for a marked decrease in urinary. The data and the family study suggest that this condition is a proximal tubular disorder with atuosomal recessive inheritance."}
{"id": "PMID:485771", "title": "[Unusual sequelae of a cranial injury].", "content": "A 13 year old child who had an apparently mild head injury presented with psychiatric sequelae. The main feature was a variable level of consciousness. The only explanation for the symptoms lay within the family environment. A hysterical converion symptom was possible but the start of a psychosis could not be excluded.", "contents": "[Unusual sequelae of a cranial injury]. A 13 year old child who had an apparently mild head injury presented with psychiatric sequelae. The main feature was a variable level of consciousness. The only explanation for the symptoms lay within the family environment. A hysterical converion symptom was possible but the start of a psychosis could not be excluded."}
{"id": "PMID:485772", "title": "[Neonatal renal papillary necrosis].", "content": "Renal papillary necrosis was detected early by plasma and urine analyses in a neonate with the respiratory distress syndrome. The intravenous urogram demonstrated characteristic features of the condition. Initial symptoms were polyuria, urinary salt loss and haematuria, but the only residual abnormality was a mild defect in urinary concentrating ability.", "contents": "[Neonatal renal papillary necrosis]. Renal papillary necrosis was detected early by plasma and urine analyses in a neonate with the respiratory distress syndrome. The intravenous urogram demonstrated characteristic features of the condition. Initial symptoms were polyuria, urinary salt loss and haematuria, but the only residual abnormality was a mild defect in urinary concentrating ability."}
{"id": "PMID:485773", "title": "[Pulmonary aspergillosis and chronic septic granulomatosis].", "content": "Two children with chronic granulomatous disease who developed diffuse pulmonary aspergillosis are described. The outcome was satisfactory in one case with miliary disease because the diagnosis was made early by an open lung biopsy. In the other case the diagnosis was delayed and the child died after 7 months with disseminated haematogenous spread of the fungal infection. Although most of the infections of chronic granulomatous disease are bacterial, the abnormalities of phagocyte killing will also predispose to fungal infections. The prolonged survival of affected children because of antibiotic therapy will increase the risk of parasitic and fungal infections.", "contents": "[Pulmonary aspergillosis and chronic septic granulomatosis]. Two children with chronic granulomatous disease who developed diffuse pulmonary aspergillosis are described. The outcome was satisfactory in one case with miliary disease because the diagnosis was made early by an open lung biopsy. In the other case the diagnosis was delayed and the child died after 7 months with disseminated haematogenous spread of the fungal infection. Although most of the infections of chronic granulomatous disease are bacterial, the abnormalities of phagocyte killing will also predispose to fungal infections. The prolonged survival of affected children because of antibiotic therapy will increase the risk of parasitic and fungal infections."}
{"id": "PMID:485775", "title": "Biologic heterogeneity and psychiatrict research. Platelet MAO activity as a case study.", "content": "This article examines current research strategies in biological psychiatry and the possible effects of biological heterogeneity on these strategies. First, the limited power of t test comparisons of measurements obtained from groups of patients and controls is demonstrated through a computer simulation. Second, we examine statistically the data from 14 recent platelet MAO studies to see if the heterogeneity in results of these studies could relate to an underlying biological heterogeneity. Finally, we suggest research methods we believe may be more useful than the current standard paradigm for elucidating the biological etiologies of psychiatric syndromes.", "contents": "Biologic heterogeneity and psychiatrict research. Platelet MAO activity as a case study. This article examines current research strategies in biological psychiatry and the possible effects of biological heterogeneity on these strategies. First, the limited power of t test comparisons of measurements obtained from groups of patients and controls is demonstrated through a computer simulation. Second, we examine statistically the data from 14 recent platelet MAO studies to see if the heterogeneity in results of these studies could relate to an underlying biological heterogeneity. Finally, we suggest research methods we believe may be more useful than the current standard paradigm for elucidating the biological etiologies of psychiatric syndromes."}
{"id": "PMID:485776", "title": "Reliability and descriptive validity of PSE syndromes.", "content": "Despite extensive research use of the Present State Examination (PSE), the validity of classification based on PSE data has not been studied extensively. We have examined a consecutive series of functional psychiatric admissions using the PSE and systematically gathered clinical and demographic data in order to study not only the reliability but the descriptive (construct) validity of classification based on PSE data. We have found that the PSE can be used in a psychiatric hospital to reliably describe and classify schizophrenic and affective syndromes with considerable descriptive validity in terms of clinical and demographic variables. We believe that this type of validity is an important step in establishing validity of clinical disease entities. The interrelationship among different kinds of validity (descriptive, concurrent, predictive) might provide clinical disease concepts with more definitive validity.", "contents": "Reliability and descriptive validity of PSE syndromes. Despite extensive research use of the Present State Examination (PSE), the validity of classification based on PSE data has not been studied extensively. We have examined a consecutive series of functional psychiatric admissions using the PSE and systematically gathered clinical and demographic data in order to study not only the reliability but the descriptive (construct) validity of classification based on PSE data. We have found that the PSE can be used in a psychiatric hospital to reliably describe and classify schizophrenic and affective syndromes with considerable descriptive validity in terms of clinical and demographic variables. We believe that this type of validity is an important step in establishing validity of clinical disease entities. The interrelationship among different kinds of validity (descriptive, concurrent, predictive) might provide clinical disease concepts with more definitive validity."}
{"id": "PMID:485777", "title": "Comparative evaluation of research diagnostic criteria for schizophrenia.", "content": "Criteria for establishing a diagnosis of schizophrenia have been proposed by several different authors. This study compared six different research diagnostic criteria (RDC) in a series of 166 patients who received a clinical diagnosis of schizophrenia in a multicenter study. The alternative criteria differed in the proportion of clinical diagnoses that were confirmed, with the Washington University (Feighner) criteria accepting only 26%. The criteria also disagreed concerning which particular patients qualified for the diagnosis. The Washington University and New York RDC disagreed 50% of the time, and some other disagreement rates were even higher. More evaluative research is needed before arbitrary criteria are permitted to redefine the concept of schizophrenia.", "contents": "Comparative evaluation of research diagnostic criteria for schizophrenia. Criteria for establishing a diagnosis of schizophrenia have been proposed by several different authors. This study compared six different research diagnostic criteria (RDC) in a series of 166 patients who received a clinical diagnosis of schizophrenia in a multicenter study. The alternative criteria differed in the proportion of clinical diagnoses that were confirmed, with the Washington University (Feighner) criteria accepting only 26%. The criteria also disagreed concerning which particular patients qualified for the diagnosis. The Washington University and New York RDC disagreed 50% of the time, and some other disagreement rates were even higher. More evaluative research is needed before arbitrary criteria are permitted to redefine the concept of schizophrenia."}
{"id": "PMID:485778", "title": "A comparison of DSM-II and DSM-III in the diagnosis of childhood psychiatric disorders. I. Agreement with expected diagnosis.", "content": "This study was conducted to compare DSM-II and DSM-III in the diagnosis of childhood and adolescent psychiatric disorders. Twenty psychiatrist-raters completed standardized diagnostic questionnaires for 24 actual case histories. This report, the first of four, presents the rater agreement with the \"expected diagnosis,\" ie, the diagnosis that we considered most appropriate for each case. The average rater agreement with the expected diagnosis was less than 50%. It was highest in cases of mental retardation, psychosis, hyperactivity, and conduct disorder. In only five cases did the most common diagnosis of the raters differ from the expected diagnosis. Analyses of these cases and those we selected to present specific diagnostic problems to the raters have produced suggestions to improve the reliability of DSM-III.", "contents": "A comparison of DSM-II and DSM-III in the diagnosis of childhood psychiatric disorders. I. Agreement with expected diagnosis. This study was conducted to compare DSM-II and DSM-III in the diagnosis of childhood and adolescent psychiatric disorders. Twenty psychiatrist-raters completed standardized diagnostic questionnaires for 24 actual case histories. This report, the first of four, presents the rater agreement with the \"expected diagnosis,\" ie, the diagnosis that we considered most appropriate for each case. The average rater agreement with the expected diagnosis was less than 50%. It was highest in cases of mental retardation, psychosis, hyperactivity, and conduct disorder. In only five cases did the most common diagnosis of the raters differ from the expected diagnosis. Analyses of these cases and those we selected to present specific diagnostic problems to the raters have produced suggestions to improve the reliability of DSM-III."}
{"id": "PMID:485779", "title": "A comparison of DSM-II and DSM-III in the diagnosis of childhood psychiatric disorders. II. Interrater agreement.", "content": "A case-history format was utilized to compare interrater agreement on childhood and adolescent psychiatric disorders, using DSM-II and DSM-III. The average interrater agreement was 57% for DSM-II and 54% for axis I (clinical psychiatric syndrome) of DSM-III. There was high agreement in both systems on cases of psychosis, conduct disorder, hyperactivity, and mental retardation, with DSM-III appearing slightly better. There was noteworthy interrater disagreement in both systems for \"anxiety\" disorders, complex cases, and in the subtyping of depression. Overall, the reliability of DSM-III appears to be good and is comparable with that of DSM-II and other classification systems of childhood psychiatric disorders.", "contents": "A comparison of DSM-II and DSM-III in the diagnosis of childhood psychiatric disorders. II. Interrater agreement. A case-history format was utilized to compare interrater agreement on childhood and adolescent psychiatric disorders, using DSM-II and DSM-III. The average interrater agreement was 57% for DSM-II and 54% for axis I (clinical psychiatric syndrome) of DSM-III. There was high agreement in both systems on cases of psychosis, conduct disorder, hyperactivity, and mental retardation, with DSM-III appearing slightly better. There was noteworthy interrater disagreement in both systems for \"anxiety\" disorders, complex cases, and in the subtyping of depression. Overall, the reliability of DSM-III appears to be good and is comparable with that of DSM-II and other classification systems of childhood psychiatric disorders."}
{"id": "PMID:485780", "title": "A comparison of DSM-II and DSM-III in the diagnosis of childhood psychiatric disorders. III. Multiaxial features.", "content": "A major feature of DSM-III is its multiaxial format. One purpose of this study was to examine the purported advantages of a multiaxial system as compared to those of a multicategory system, eg., DSM-II. We found that the multiaxial system led to a more complete and reliable diagnosis of complex clinical cases. Concomitant medical disorder and psychosocial stressors were coded with high levels of interrater agreement. We conclude that the multiaxial framework is a major strength of DSM-III.", "contents": "A comparison of DSM-II and DSM-III in the diagnosis of childhood psychiatric disorders. III. Multiaxial features. A major feature of DSM-III is its multiaxial format. One purpose of this study was to examine the purported advantages of a multiaxial system as compared to those of a multicategory system, eg., DSM-II. We found that the multiaxial system led to a more complete and reliable diagnosis of complex clinical cases. Concomitant medical disorder and psychosocial stressors were coded with high levels of interrater agreement. We conclude that the multiaxial framework is a major strength of DSM-III."}
{"id": "PMID:485781", "title": "Development of psychological distress among young adults.", "content": "Persons with high scores on psychiatric screening scales appear frequently in general medical settings, psychiatric outpatient clinics, and other agencies providing assistance, but there is considerable controversy concerning the appropriate interpretation of high scores on such scales obtained in community epidemiological studies. Data are presented from a 16-year follow-up study of children, first studied in 1961, examining developmental aspects of achieving high scores on such scales. Data come from the children themselves, their mothers, teachers, and school records. The findings are consistent with the hypothesis that such generalized distress reports are in part a learned pattern of illness behavior involving a focus on internal feeling states, careful monitoring of body sensations, and a high level of self-awareness.", "contents": "Development of psychological distress among young adults. Persons with high scores on psychiatric screening scales appear frequently in general medical settings, psychiatric outpatient clinics, and other agencies providing assistance, but there is considerable controversy concerning the appropriate interpretation of high scores on such scales obtained in community epidemiological studies. Data are presented from a 16-year follow-up study of children, first studied in 1961, examining developmental aspects of achieving high scores on such scales. Data come from the children themselves, their mothers, teachers, and school records. The findings are consistent with the hypothesis that such generalized distress reports are in part a learned pattern of illness behavior involving a focus on internal feeling states, careful monitoring of body sensations, and a high level of self-awareness."}
{"id": "PMID:485782", "title": "Pretraining for group psychotherapy. A cognitive-experiential approach.", "content": "Pretherapy training for group psychotherapy is conceptualized in terms of learned conditions of preparedness (objectives) and in terms of methods of preparation (procedures). Controlled studies of pretraining for group psychotherapy were reviewed in reference to these two dimensions and their effects. A controlled study of pretraining involving five outpatient therapy groups that used a new cognitive-experiential approach to pretraining was performed. Pretrained groups had significantly higher patient attendance and significantly fewer dropouts than nonpretrained groups.", "contents": "Pretraining for group psychotherapy. A cognitive-experiential approach. Pretherapy training for group psychotherapy is conceptualized in terms of learned conditions of preparedness (objectives) and in terms of methods of preparation (procedures). Controlled studies of pretraining for group psychotherapy were reviewed in reference to these two dimensions and their effects. A controlled study of pretraining involving five outpatient therapy groups that used a new cognitive-experiential approach to pretraining was performed. Pretrained groups had significantly higher patient attendance and significantly fewer dropouts than nonpretrained groups."}
{"id": "PMID:485783", "title": "The psychological treatment of depression. Evidence for the efficacy of psychotherapy alone, in comparison with, and in combination with pharmacotherapy.", "content": "Seventeen clinical trials are identified that test the efficacy of various psychological treatments (behavioral, cognitive, group, marital, interpersonal) alone, in comparison with, and in combination with pharmacotherapy in homogeneous samples of depressed outpatients. I describe the results of these studies, gaps in the evidence, and suggestions for future research directions.", "contents": "The psychological treatment of depression. Evidence for the efficacy of psychotherapy alone, in comparison with, and in combination with pharmacotherapy. Seventeen clinical trials are identified that test the efficacy of various psychological treatments (behavioral, cognitive, group, marital, interpersonal) alone, in comparison with, and in combination with pharmacotherapy in homogeneous samples of depressed outpatients. I describe the results of these studies, gaps in the evidence, and suggestions for future research directions."}
{"id": "PMID:485786", "title": "Cloward's anterior fusion in the treatment of cervical spinal traumatic injury and degeneration.", "content": "The aim of this study was to evaluate the long-term results of Cloward's anterior interbody fusion of the cervical spine and to identify the factors influencing them. The series consisted of 29 patients operated on in 1968--75. The indication for operation was in 12 cases intensive radicular symptoms, not responding to conservative treatment, in connection with considerable degeneration of the corresponding spinal segment only, and in 17 cases instability of the cervical spine caused by traumatic injury followed by dislocation and radicular or medullary symptoms enhancing in spite of conservative treatment by skull traction or collar. Twenty-five patients (86%) attended follow-up after an average time lapse of 6.5 years from operation. The operative result was evaluated considering objective neurological improvement, subjective improvement, present symptoms and working capacity. The operative result was at least fair in 7/11 in the degeneration group and in 12/14 in the traumatic injury group. All fusions were radiologically successful. Adequate correction of a primary flexion deformity of more than 15 degrees was not achieved. Age over 35 years and motor defect preoperatively proved to be statistically significant prognostic factors for a poor operative result in the traumatic injury group. Preoperative sick-leaves and a duration of preoperative symptoms exceeding six months proved to be prognostic factors for a poor result in the degeneration group. In spite of the relatively good clinical results obtained, this study does not justify any conclusions concerning the value of Cloward's procedure compared to other methods of treatment, since no control material was available.", "contents": "Cloward's anterior fusion in the treatment of cervical spinal traumatic injury and degeneration. The aim of this study was to evaluate the long-term results of Cloward's anterior interbody fusion of the cervical spine and to identify the factors influencing them. The series consisted of 29 patients operated on in 1968--75. The indication for operation was in 12 cases intensive radicular symptoms, not responding to conservative treatment, in connection with considerable degeneration of the corresponding spinal segment only, and in 17 cases instability of the cervical spine caused by traumatic injury followed by dislocation and radicular or medullary symptoms enhancing in spite of conservative treatment by skull traction or collar. Twenty-five patients (86%) attended follow-up after an average time lapse of 6.5 years from operation. The operative result was evaluated considering objective neurological improvement, subjective improvement, present symptoms and working capacity. The operative result was at least fair in 7/11 in the degeneration group and in 12/14 in the traumatic injury group. All fusions were radiologically successful. Adequate correction of a primary flexion deformity of more than 15 degrees was not achieved. Age over 35 years and motor defect preoperatively proved to be statistically significant prognostic factors for a poor operative result in the traumatic injury group. Preoperative sick-leaves and a duration of preoperative symptoms exceeding six months proved to be prognostic factors for a poor result in the degeneration group. In spite of the relatively good clinical results obtained, this study does not justify any conclusions concerning the value of Cloward's procedure compared to other methods of treatment, since no control material was available."}
{"id": "PMID:485787", "title": "[Delayed complications after dislocations and fractures in the pelvic region (author's transl)].", "content": "In the case of surgically dressed necroses of the hip condyle and coxarthroses a condition was found after fracture of the pelvis or after dislocation of the hip joint in 7.2% of the cases. In 13.2% of the cases a trauma was found anamnestically. It was subdivided into fractures of the acetabulum, fractures of the pelvic girdle, dislocations, and fractures of the pelvic brim on the basis of the system of Judet and Engler as well as Feldkamp. Fractures of the pelvic brim through muscular traction, traction of the ligament, and direct trauma were discussed. The most frequent delayed consequences arising after dislocations of the hip joint and fractures of the acetabulum are: necroses of the head of the femur, coxarthroses, pseudarthroses, and myositis ossificans. The pathogenesis of the delayed injuries resulted from: capsular and vascular lesions, damage to cartilage with fractures in the spongiosa region, incongruence of the surface of the joint, insufficiency of the static structure of the pelvic ring, deposit of calcium salt outside the bone. In some cases the progress of the necrosis could be arrested by means of osteotomy and spongiosa filling. The rate of necrosis increases in proportion to the period between the time of occurrence of the accident and reposition (limit of 6 h). Even though about half the pelvic girdle fractures show good results with conservative treatment, in the case of fractures of the acetabulum a reconstruction as far as possible continuous and without stages is to be aimed at. In many cases the development of a myositis ossificans cannot be prevented.", "contents": "[Delayed complications after dislocations and fractures in the pelvic region (author's transl)]. In the case of surgically dressed necroses of the hip condyle and coxarthroses a condition was found after fracture of the pelvis or after dislocation of the hip joint in 7.2% of the cases. In 13.2% of the cases a trauma was found anamnestically. It was subdivided into fractures of the acetabulum, fractures of the pelvic girdle, dislocations, and fractures of the pelvic brim on the basis of the system of Judet and Engler as well as Feldkamp. Fractures of the pelvic brim through muscular traction, traction of the ligament, and direct trauma were discussed. The most frequent delayed consequences arising after dislocations of the hip joint and fractures of the acetabulum are: necroses of the head of the femur, coxarthroses, pseudarthroses, and myositis ossificans. The pathogenesis of the delayed injuries resulted from: capsular and vascular lesions, damage to cartilage with fractures in the spongiosa region, incongruence of the surface of the joint, insufficiency of the static structure of the pelvic ring, deposit of calcium salt outside the bone. In some cases the progress of the necrosis could be arrested by means of osteotomy and spongiosa filling. The rate of necrosis increases in proportion to the period between the time of occurrence of the accident and reposition (limit of 6 h). Even though about half the pelvic girdle fractures show good results with conservative treatment, in the case of fractures of the acetabulum a reconstruction as far as possible continuous and without stages is to be aimed at. In many cases the development of a myositis ossificans cannot be prevented."}
{"id": "PMID:485788", "title": "Measurements of cefuroxime concentration in bone in dogs and man.", "content": "Measurements of Cefuroxime levels performed in dogs show a decrease against time in well perfused tissue similar to that in serum, while in bradytrophic tissue--such as bone and articular capsule--there is a distinct rise of these values in the time scale assessed. In similar investigations in man the higher levels in bradytrophic tissue are attributable to delayed elimination from the compact and spongy bone as compared to the serum. The effect on osteomyelitis, to be derived from this, is discussed.", "contents": "Measurements of cefuroxime concentration in bone in dogs and man. Measurements of Cefuroxime levels performed in dogs show a decrease against time in well perfused tissue similar to that in serum, while in bradytrophic tissue--such as bone and articular capsule--there is a distinct rise of these values in the time scale assessed. In similar investigations in man the higher levels in bradytrophic tissue are attributable to delayed elimination from the compact and spongy bone as compared to the serum. The effect on osteomyelitis, to be derived from this, is discussed."}
{"id": "PMID:485789", "title": "Unhooking of GSB total knee during dislocation of the patella.", "content": "This is a case report of a special and undoubtedly rare complication occurring in a hinge joint with a moving instant center such as the GSB total knee joint. The preoperative situation, the history of the incident and the operative report are being described as well as a radiological follow-up. The mechanism of unhooking of the tibial shaft from its junction with the femoral stem during dislocation of the patella is being reconstructed.", "contents": "Unhooking of GSB total knee during dislocation of the patella. This is a case report of a special and undoubtedly rare complication occurring in a hinge joint with a moving instant center such as the GSB total knee joint. The preoperative situation, the history of the incident and the operative report are being described as well as a radiological follow-up. The mechanism of unhooking of the tibial shaft from its junction with the femoral stem during dislocation of the patella is being reconstructed."}
{"id": "PMID:485790", "title": "Injuries of the thoracolumbar junction. Clinical and radiological results in 149 patients.", "content": "In a series of 149 fractures of the thoracolumbar junction (Th12-L1), treated at the Central Hospital of Tampere from 1968 to 1975, 101 patients were seen after an average interval of 5 years. Over half of the fractures were sustained at home or in leisure hours. Over 90% were anterior wedge fractures. One patient died in the hospital of other injuries. The injury caused peraplegia in three cases and other neurological lesions in six. The treatment was conservative except in the patients with paraplegia, who were surgically treated. The patients were mobilized after an average of 15 days. The neurological condition did not deteriorate. Noteworthy changes of the radiological findings occurred during follow-up. The clinical result was considered good in two thirds of the patients, and 91% were able to return to work. The frequency of neurological findings was significant in patients showing radiological deformities. The force responsible for the fracture correlated with the radiological findings and the occurrence of neurological lesions. Narrowing of the vertebral canal and degenerative changes observed on admission had a significant and unfavorable effect on the clinical result.", "contents": "Injuries of the thoracolumbar junction. Clinical and radiological results in 149 patients. In a series of 149 fractures of the thoracolumbar junction (Th12-L1), treated at the Central Hospital of Tampere from 1968 to 1975, 101 patients were seen after an average interval of 5 years. Over half of the fractures were sustained at home or in leisure hours. Over 90% were anterior wedge fractures. One patient died in the hospital of other injuries. The injury caused peraplegia in three cases and other neurological lesions in six. The treatment was conservative except in the patients with paraplegia, who were surgically treated. The patients were mobilized after an average of 15 days. The neurological condition did not deteriorate. Noteworthy changes of the radiological findings occurred during follow-up. The clinical result was considered good in two thirds of the patients, and 91% were able to return to work. The frequency of neurological findings was significant in patients showing radiological deformities. The force responsible for the fracture correlated with the radiological findings and the occurrence of neurological lesions. Narrowing of the vertebral canal and degenerative changes observed on admission had a significant and unfavorable effect on the clinical result."}
{"id": "PMID:485791", "title": "Fractures of the lumbar spine. Clinical and radiological results in 94 patients.", "content": "Sixty-six (70 per cent) of 94 patients with fracture of the lumbar spine (L2-L5), treated at the Central Hospital of Tampere in 1968--1975, were seen after an average interval of 5 years and 4 months. The mean age of the patients at the time of injury was 40.4 years. Somewhat less than half the fractures were sustained at home or in leisure hours and one third were due to traffic accidents. Two thirds were anterior wedge fractures. One patient died in the hospital of other injuries sustained in the same accident. Paraplegia was caused in one case, other neurological lesions in six cases. The treatment was mainly conservative and based on the principle of early functional exercises. Surgery was used in 2 cases of fracture-dislocation. The patients were mobilized after an average of 19 days. The mean duration of hospital treatment was 26 days. Seventy-nine per cent were able to return to work after an average of 3.6 months. No noteworthy changes in the neurological state occurred during follow-up. Half the followed-up patients considered the state of their back at least good. Poor end-results were more frequent in patients over 40 years old than in those under 40. Radiological deformities were more often observed in patients showing neurological symptoms. Increased interspinous distance and flexion or compression deformities had an unfavourable effect on the clinical result.", "contents": "Fractures of the lumbar spine. Clinical and radiological results in 94 patients. Sixty-six (70 per cent) of 94 patients with fracture of the lumbar spine (L2-L5), treated at the Central Hospital of Tampere in 1968--1975, were seen after an average interval of 5 years and 4 months. The mean age of the patients at the time of injury was 40.4 years. Somewhat less than half the fractures were sustained at home or in leisure hours and one third were due to traffic accidents. Two thirds were anterior wedge fractures. One patient died in the hospital of other injuries sustained in the same accident. Paraplegia was caused in one case, other neurological lesions in six cases. The treatment was mainly conservative and based on the principle of early functional exercises. Surgery was used in 2 cases of fracture-dislocation. The patients were mobilized after an average of 19 days. The mean duration of hospital treatment was 26 days. Seventy-nine per cent were able to return to work after an average of 3.6 months. No noteworthy changes in the neurological state occurred during follow-up. Half the followed-up patients considered the state of their back at least good. Poor end-results were more frequent in patients over 40 years old than in those under 40. Radiological deformities were more often observed in patients showing neurological symptoms. Increased interspinous distance and flexion or compression deformities had an unfavourable effect on the clinical result."}
{"id": "PMID:485792", "title": "Injuries of the cervical spine. Clinical and radiological results in 119 patients.", "content": "Seventy-one patients of 119 with injury of the cervical spine were re-examined an average of 5 years and 5 months after the accident. The mean age of the patients was 39 years. There were 91 male and 28 female patients. The majority of injuries (51) were sustained by car drivers and passengers involved in traffic accidents. More than one cervical vertebra was damaged in 36 patients. Neurological lesions were observed in 59 patients; tetraplegia was present in 19 of these. The average duration of hospital treatment was 37 days. Twenty-four patients were treated at the intensive care unit. Skull traction was used in 69 cases for an average of 38 days to bring about reduction and immobilization. Reduction and anterior spondylodesis by Cloward's technique was performed on 13 patients. Of the operated patients, 11 showed root symptoms or incomplete cord lesions. The 19 tetraplegics were all conservatively treated. Fourteen patients died of their cervical spine injuries and 7 remained permanently disabled. Half the patients recovered to a pain-free state and one third became asymptomatic. Of the radiological deformities observed on admission, lowering of the disc space increased during follow-up, and forward sliding, increase in interspinous distance and narrowing of the vertebral canal decreased. The clinical result was poorer if flexion deformity, compression, forward sliding, narrowing of the vertebral canal or lowering of the disc space were observed at the last radiological examination.", "contents": "Injuries of the cervical spine. Clinical and radiological results in 119 patients. Seventy-one patients of 119 with injury of the cervical spine were re-examined an average of 5 years and 5 months after the accident. The mean age of the patients was 39 years. There were 91 male and 28 female patients. The majority of injuries (51) were sustained by car drivers and passengers involved in traffic accidents. More than one cervical vertebra was damaged in 36 patients. Neurological lesions were observed in 59 patients; tetraplegia was present in 19 of these. The average duration of hospital treatment was 37 days. Twenty-four patients were treated at the intensive care unit. Skull traction was used in 69 cases for an average of 38 days to bring about reduction and immobilization. Reduction and anterior spondylodesis by Cloward's technique was performed on 13 patients. Of the operated patients, 11 showed root symptoms or incomplete cord lesions. The 19 tetraplegics were all conservatively treated. Fourteen patients died of their cervical spine injuries and 7 remained permanently disabled. Half the patients recovered to a pain-free state and one third became asymptomatic. Of the radiological deformities observed on admission, lowering of the disc space increased during follow-up, and forward sliding, increase in interspinous distance and narrowing of the vertebral canal decreased. The clinical result was poorer if flexion deformity, compression, forward sliding, narrowing of the vertebral canal or lowering of the disc space were observed at the last radiological examination."}
{"id": "PMID:485793", "title": "A bacteriological evaluation of a programme for preoperative total body-washing with chlorhexidine gluconate performed by patients undergoing orthopaedic surgery.", "content": "Twenty-eight orthopaedic patients were taught to wash their entire bodies with chlorhexidine gluconate on two consecutive days preoperatively, starting in their homes. The operation field was also washed a third time. A bacteriological evaluation was performed using contact plates. A significant reduction of the skin flora, including Staph. aureus, was registered. No adverse side effects of total body-washing were observed.", "contents": "A bacteriological evaluation of a programme for preoperative total body-washing with chlorhexidine gluconate performed by patients undergoing orthopaedic surgery. Twenty-eight orthopaedic patients were taught to wash their entire bodies with chlorhexidine gluconate on two consecutive days preoperatively, starting in their homes. The operation field was also washed a third time. A bacteriological evaluation was performed using contact plates. A significant reduction of the skin flora, including Staph. aureus, was registered. No adverse side effects of total body-washing were observed."}
{"id": "PMID:485794", "title": "[Fracture healing and folic acid (author's transl)].", "content": "The reaction of folic acid on fracture healing was tested on the basis of 113 rabbits. In both cases, the only application of folic acid and the combination with amino acids, the results can be regarded positively. This is also true when we consider possible methodic faults.", "contents": "[Fracture healing and folic acid (author's transl)]. The reaction of folic acid on fracture healing was tested on the basis of 113 rabbits. In both cases, the only application of folic acid and the combination with amino acids, the results can be regarded positively. This is also true when we consider possible methodic faults."}
{"id": "PMID:485795", "title": "Treatment of periarticular calcification after total hip arthroplasty.", "content": "Calcification after total hip arthroplasty resulting in severely impaired function requiring reoperation is rare. This is a report on one patient with wide spread calcification where the patient was treated with Indometacin and the total hip exchanged.", "contents": "Treatment of periarticular calcification after total hip arthroplasty. Calcification after total hip arthroplasty resulting in severely impaired function requiring reoperation is rare. This is a report on one patient with wide spread calcification where the patient was treated with Indometacin and the total hip exchanged."}
{"id": "PMID:485796", "title": "Excisional biopsy for bone tumours.", "content": "Compartmental muscular resections without open biopsy is a common procedure for soft tissue tumours suspected of malignancy. In bone tumours, where the diagnosis is supposed to be sarcoma, an excisional biopsy is seldom possible without severe recontructive problems and it may be unnecessarily mutilating should the tumour be benign. For the fibula, the clavicula, metatarsal and metacarpal bones, the distal third of ulna and the proximal third of radius, however, excisional biopsy as the primary procedure should be taken into account. The resulting loss of function is minor and can well be accepted even if the tumour turns out to be benign. On the other hand, if it is malignant as supposed, the radical excisional biopsy saves the patient from amputation. When incisional biopsy is used instead of excisional biopsy the definite surgery has to be made much wider and will often be mutilating. A case of chondrosarcoma illustrates the advantage and the disadvantage of this principle as well as an unusual reconstruction.", "contents": "Excisional biopsy for bone tumours. Compartmental muscular resections without open biopsy is a common procedure for soft tissue tumours suspected of malignancy. In bone tumours, where the diagnosis is supposed to be sarcoma, an excisional biopsy is seldom possible without severe recontructive problems and it may be unnecessarily mutilating should the tumour be benign. For the fibula, the clavicula, metatarsal and metacarpal bones, the distal third of ulna and the proximal third of radius, however, excisional biopsy as the primary procedure should be taken into account. The resulting loss of function is minor and can well be accepted even if the tumour turns out to be benign. On the other hand, if it is malignant as supposed, the radical excisional biopsy saves the patient from amputation. When incisional biopsy is used instead of excisional biopsy the definite surgery has to be made much wider and will often be mutilating. A case of chondrosarcoma illustrates the advantage and the disadvantage of this principle as well as an unusual reconstruction."}
{"id": "PMID:485797", "title": "Quality of care for completed stroke without rehabilitation: evaluation by assessing patient outcomes.", "content": "Using the method of quality assurance of stroke rehabilitation developed at the University of Minnesota (UMn) by assessing patient outcomes on the basis of a modification of the Williamson Functional Limitation Scale (WFLS), 84 patients (group H) with completed stroke were surveyed 8 months to 13 years following treatment without rehabilitation at Hennepin County General Hospital. Because a greater percentage (42%) than estimated (29%) had died, the percentage of those not independent in self-care (31%) was less than estimated (46%). These patients were not matched with a group given rehabilitation (group U) previously studied at UMn but were generally less severely involved, although older on the average. However, even so, only 47% of those still living were independent in self-care in group H in contrast to 69% in group U. Also 42% of group H were in nursing homes or board-and-care facilities while only 18% of the rehabilitated group were. Although the modified WFLS is used in outcome studies for quality of rehabilitation, it is even sensitive enough to show differences in outcomes in a comparative study such as this.", "contents": "Quality of care for completed stroke without rehabilitation: evaluation by assessing patient outcomes. Using the method of quality assurance of stroke rehabilitation developed at the University of Minnesota (UMn) by assessing patient outcomes on the basis of a modification of the Williamson Functional Limitation Scale (WFLS), 84 patients (group H) with completed stroke were surveyed 8 months to 13 years following treatment without rehabilitation at Hennepin County General Hospital. Because a greater percentage (42%) than estimated (29%) had died, the percentage of those not independent in self-care (31%) was less than estimated (46%). These patients were not matched with a group given rehabilitation (group U) previously studied at UMn but were generally less severely involved, although older on the average. However, even so, only 47% of those still living were independent in self-care in group H in contrast to 69% in group U. Also 42% of group H were in nursing homes or board-and-care facilities while only 18% of the rehabilitated group were. Although the modified WFLS is used in outcome studies for quality of rehabilitation, it is even sensitive enough to show differences in outcomes in a comparative study such as this."}
{"id": "PMID:485798", "title": "Computed tomography: its potential as a predictor of functional recovery following stroke.", "content": "In a 6-month period, 40 consecutive patients with a diagnosis of cerebral infarction had computed tomography (CT) scanning. The purpose of this study was to evaluate the association between the location and size of the lesion on CT scan and the functional status of the patient on discharge and follow-up. The results of the CT scan were divided into the following major groups: deep (involving the basal ganglia, internal capsule and thalamus), superficial large and superficial small (involving the cerebral hemisphere up to and including the external capsule) and normal. At discharge, patients were divided into 2 major functional groups: group A, minimal assist to independent in transfers and ambulation; and group B, moderate to maximal assist in transfers and maximal assist in ambulation. Ten of 11 patients with small superficial lesions were in group A, and 10 of 13 patients with deep lesions were in group B. Of 10 patients with large superficial lesions, 5 were in group A and 5 in group B. All 6 patients with normal CT scans results were in group A. Thus, results of CT scanning appear to be associated with degree of functional recovery.", "contents": "Computed tomography: its potential as a predictor of functional recovery following stroke. In a 6-month period, 40 consecutive patients with a diagnosis of cerebral infarction had computed tomography (CT) scanning. The purpose of this study was to evaluate the association between the location and size of the lesion on CT scan and the functional status of the patient on discharge and follow-up. The results of the CT scan were divided into the following major groups: deep (involving the basal ganglia, internal capsule and thalamus), superficial large and superficial small (involving the cerebral hemisphere up to and including the external capsule) and normal. At discharge, patients were divided into 2 major functional groups: group A, minimal assist to independent in transfers and ambulation; and group B, moderate to maximal assist in transfers and maximal assist in ambulation. Ten of 11 patients with small superficial lesions were in group A, and 10 of 13 patients with deep lesions were in group B. Of 10 patients with large superficial lesions, 5 were in group A and 5 in group B. All 6 patients with normal CT scans results were in group A. Thus, results of CT scanning appear to be associated with degree of functional recovery."}
{"id": "PMID:485799", "title": "Isokinetic torque levels for high school football players.", "content": "One hundred fifteen high school football players were screened isokinetically for the knee extensor and flexor torque generating capabilities and muscle imbalances. Each player was tested on a Cybex II at speeds of 30 degrees/sec and 180 degrees/sec. The subjects were stratified by age (15, 16, 17) and by position (linemen vs receivers and backs). Significant (p less than 0.05) torque differences were observed at both the slow and fast speeds for the knee extensors and flexors across the 3 age groups. However, the flexion to extension ratios did not differ significantly. On the other hand, when considering the positions, flexor torque and flexion to extension ratio at the slow speed were greater (p less than 0.05) for the linemen than the receivers and backs. Partial correlation analyses showed that body weight had the greatest effect on the knee torque values. It was concluded that basic torque measures differed with age and by position in the sport of football. Also, speed of movement altered the force generating capabilities of the muscle and resultant ratios.", "contents": "Isokinetic torque levels for high school football players. One hundred fifteen high school football players were screened isokinetically for the knee extensor and flexor torque generating capabilities and muscle imbalances. Each player was tested on a Cybex II at speeds of 30 degrees/sec and 180 degrees/sec. The subjects were stratified by age (15, 16, 17) and by position (linemen vs receivers and backs). Significant (p less than 0.05) torque differences were observed at both the slow and fast speeds for the knee extensors and flexors across the 3 age groups. However, the flexion to extension ratios did not differ significantly. On the other hand, when considering the positions, flexor torque and flexion to extension ratio at the slow speed were greater (p less than 0.05) for the linemen than the receivers and backs. Partial correlation analyses showed that body weight had the greatest effect on the knee torque values. It was concluded that basic torque measures differed with age and by position in the sport of football. Also, speed of movement altered the force generating capabilities of the muscle and resultant ratios."}
{"id": "PMID:485800", "title": "Physiologic effects on adult men of circuit strength training and jogging.", "content": "The purpose of this study was to determine the effects of 8 weeks of circuit strength training (CST) followed by 8 weeks of jogging and then 8 weeks, of CST or jogging. During the final 8 weeks, the subjects were randomly assigned to either CST or jogging groups. The subjects (n=16, x age = 29 yrs) exercised 3 days/week. The strength training involved 2 circuits of reciprocal exercises using isokinetic devices with 10 to 15 repetitions/set and 30 seconds of rest between sets. The subjects jogged 3 miles/day during the jogging program. After the initial 8 weeks of CST, significant changes were found in treadmill performance time, maximum oxygen uptake (VO2max), maximum pulmonary ventilation (VEmax), body fat, total skinfold fat, fat weight, lean weight, isotonic bench and leg press, and isokinetic slow speed, fast speed, and power endurance measures. The jogging program elicited significantly greater changes in treadmill performance time and VO2max. Further reductions were found in total skinfolds and waist girth during the jogging program. Leg strength was maintained during jogging but upper body strength was reduced significantly. Physiologic levels were maintained during the final 8 weeks and showed no differences between the CST and jogging groups.", "contents": "Physiologic effects on adult men of circuit strength training and jogging. The purpose of this study was to determine the effects of 8 weeks of circuit strength training (CST) followed by 8 weeks of jogging and then 8 weeks, of CST or jogging. During the final 8 weeks, the subjects were randomly assigned to either CST or jogging groups. The subjects (n=16, x age = 29 yrs) exercised 3 days/week. The strength training involved 2 circuits of reciprocal exercises using isokinetic devices with 10 to 15 repetitions/set and 30 seconds of rest between sets. The subjects jogged 3 miles/day during the jogging program. After the initial 8 weeks of CST, significant changes were found in treadmill performance time, maximum oxygen uptake (VO2max), maximum pulmonary ventilation (VEmax), body fat, total skinfold fat, fat weight, lean weight, isotonic bench and leg press, and isokinetic slow speed, fast speed, and power endurance measures. The jogging program elicited significantly greater changes in treadmill performance time and VO2max. Further reductions were found in total skinfolds and waist girth during the jogging program. Leg strength was maintained during jogging but upper body strength was reduced significantly. Physiologic levels were maintained during the final 8 weeks and showed no differences between the CST and jogging groups."}
{"id": "PMID:485801", "title": "Physiologic responses to exercise in myocardial infarction patients following residential rehabilitation.", "content": "The physiological responses to exercise were studied in 16 men (33-52 years of age), 8--12 weeks after a first uncomplicated myocardial infarction and following a 3--4 week period of attendance at a residential rehabilitation center at which the patients were required to participate in a controlled program of exercise, sport and recreation. Data were also collected on an inactive, but otherwise healthy group of men of the same age and occupational status, and on an occupationally and recreationally active healthy group. The patient group were indistinguishable from the healthy inactive group in their response to submaximal exercise, although both of these groups showed differences when compared with the active group. Symptom-limited maximal data were also collected and these are reported in relation to the energy requirements of some common leisure, occupational and domestic activities.", "contents": "Physiologic responses to exercise in myocardial infarction patients following residential rehabilitation. The physiological responses to exercise were studied in 16 men (33-52 years of age), 8--12 weeks after a first uncomplicated myocardial infarction and following a 3--4 week period of attendance at a residential rehabilitation center at which the patients were required to participate in a controlled program of exercise, sport and recreation. Data were also collected on an inactive, but otherwise healthy group of men of the same age and occupational status, and on an occupationally and recreationally active healthy group. The patient group were indistinguishable from the healthy inactive group in their response to submaximal exercise, although both of these groups showed differences when compared with the active group. Symptom-limited maximal data were also collected and these are reported in relation to the energy requirements of some common leisure, occupational and domestic activities."}
{"id": "PMID:485802", "title": "Isometric muscle contraction response during recovery from reduced intramuscular temperature.", "content": "This study was done to determine the effects of a 30-minute cold water bath on intramuscular temperature and plantar flexion strength, immediately after treatment and during a 3-hour posttreatment recovery period. Twenty persons were tested twice, receiving treatment once and serving as controls once. Measurements were taken prior to the treatment period, immediately after treatment and then every 30 minutes for 3 hours. The dominant leg was submerged in water at 10 C for 30 minutes. Plantar flexion strength was measured with a cable tensiometer and intramuscular temperature was measured with a hypodermic thermistor probe. Intramuscular temperature significantly decreased immediately posttreatment on the experimental days and then increased significantly during the recovery period. Significant increases in strength were noted during the recovery period. A definite relationship exists between intramuscular temperature and plantar flexion strength.", "contents": "Isometric muscle contraction response during recovery from reduced intramuscular temperature. This study was done to determine the effects of a 30-minute cold water bath on intramuscular temperature and plantar flexion strength, immediately after treatment and during a 3-hour posttreatment recovery period. Twenty persons were tested twice, receiving treatment once and serving as controls once. Measurements were taken prior to the treatment period, immediately after treatment and then every 30 minutes for 3 hours. The dominant leg was submerged in water at 10 C for 30 minutes. Plantar flexion strength was measured with a cable tensiometer and intramuscular temperature was measured with a hypodermic thermistor probe. Intramuscular temperature significantly decreased immediately posttreatment on the experimental days and then increased significantly during the recovery period. Significant increases in strength were noted during the recovery period. A definite relationship exists between intramuscular temperature and plantar flexion strength."}
{"id": "PMID:485803", "title": "Musculocutaneous nerve palsy: an isolated complication of surgery.", "content": "Isolated paralysis of the musculocutaneous nerve, as reported in the literature, is an extremely rare phenomenon. It is our purpose to document the occurrence of one case as a complication of abdominal surgery. Possible mechanisms suggest why the musculocutaneous nerve may be vulnerable to injury during surgical procedures that incorporate certain methods of patient positioning. The particular pattern of nerve damage reflected in this case report may be helpful in further elucidating the other, more common, diffuse postoperative brachial plexus palsies.", "contents": "Musculocutaneous nerve palsy: an isolated complication of surgery. Isolated paralysis of the musculocutaneous nerve, as reported in the literature, is an extremely rare phenomenon. It is our purpose to document the occurrence of one case as a complication of abdominal surgery. Possible mechanisms suggest why the musculocutaneous nerve may be vulnerable to injury during surgical procedures that incorporate certain methods of patient positioning. The particular pattern of nerve damage reflected in this case report may be helpful in further elucidating the other, more common, diffuse postoperative brachial plexus palsies."}
{"id": "PMID:485804", "title": "Osteolysis of the ribs in progressive systemic sclerosis.", "content": "In a patient with progressive systemic sclerosis (PSS), osteolysis of the posterior portion of the rib cage developed in an insidious fashion, without symptoms or preceding trauma. Six previous examples of rib resorption in PSS are reviewed. The destructive mechanism is unknown but may be related to endarteritis and ischemia.", "contents": "Osteolysis of the ribs in progressive systemic sclerosis. In a patient with progressive systemic sclerosis (PSS), osteolysis of the posterior portion of the rib cage developed in an insidious fashion, without symptoms or preceding trauma. Six previous examples of rib resorption in PSS are reviewed. The destructive mechanism is unknown but may be related to endarteritis and ischemia."}
{"id": "PMID:485806", "title": "EMG biofeedback in stroke: effect of patient characteristics.", "content": "Forty-eight upper and 44 lower extremities of 52 stroke patients were treated using a clinical emg biofeedback training approach. The age, sex, hemiparetic side, duration of stroke or previous rehabilitation, and number of biofeedback training sessions had no significant relationship to treatment outcomes. Lower extremities responded more favorably to training than upper extremities, and the prospects for successful treatments in the upper limb were further diminished when proprioceptive impairments were present. Possible explanations for the poorer responsiveness of the upper extremity to emg biofeedback training are provided, the importance of a motivational element is stressed, and a suggestion is offered for the direction of future work designed to predict the value in applying this modality.", "contents": "EMG biofeedback in stroke: effect of patient characteristics. Forty-eight upper and 44 lower extremities of 52 stroke patients were treated using a clinical emg biofeedback training approach. The age, sex, hemiparetic side, duration of stroke or previous rehabilitation, and number of biofeedback training sessions had no significant relationship to treatment outcomes. Lower extremities responded more favorably to training than upper extremities, and the prospects for successful treatments in the upper limb were further diminished when proprioceptive impairments were present. Possible explanations for the poorer responsiveness of the upper extremity to emg biofeedback training are provided, the importance of a motivational element is stressed, and a suggestion is offered for the direction of future work designed to predict the value in applying this modality."}
{"id": "PMID:485811", "title": "A psychological profile of the sexual dysfunctions.", "content": "The present report summarizes an assessment of the psychological characteristics of a sample of sexually dysfunctional patients and compares them to those of a sample of heterosexual normals. Forty-seven male and 40 female dysfunctional patients were evaluated on the Derogatis Sexual Functioning Inventory (DSFI) and contrasted to a group of 200 heterosexual normals. Findings indicated substantial differences between dysfunctionals and normals on seven of the eight subtests of the DSFI. Both male and female patients showed higher levels of psychological distress and dysphoric affect than normals, and also revealed decrements on sexual information. Particularly noteworthy among male dysfunctionals were lowered sexual drive levels and a somewhat constricted repertoire of sexual experiences. Male dysfunctionals also revealed gender role definitions less polarized in the masculine direction. Female patients did not reveal any additional decrements beyond those mentioned previously, and some equivocation in defining gender role. However, detailed analyses of Experience and Fantasy subtests depicted female patients as more creative and less constrained in their sexual activities. Conclusions suggest that sexually dysfunctional patients do maintain a characteristic psychological profile but one that is distinct for males and females.", "contents": "A psychological profile of the sexual dysfunctions. The present report summarizes an assessment of the psychological characteristics of a sample of sexually dysfunctional patients and compares them to those of a sample of heterosexual normals. Forty-seven male and 40 female dysfunctional patients were evaluated on the Derogatis Sexual Functioning Inventory (DSFI) and contrasted to a group of 200 heterosexual normals. Findings indicated substantial differences between dysfunctionals and normals on seven of the eight subtests of the DSFI. Both male and female patients showed higher levels of psychological distress and dysphoric affect than normals, and also revealed decrements on sexual information. Particularly noteworthy among male dysfunctionals were lowered sexual drive levels and a somewhat constricted repertoire of sexual experiences. Male dysfunctionals also revealed gender role definitions less polarized in the masculine direction. Female patients did not reveal any additional decrements beyond those mentioned previously, and some equivocation in defining gender role. However, detailed analyses of Experience and Fantasy subtests depicted female patients as more creative and less constrained in their sexual activities. Conclusions suggest that sexually dysfunctional patients do maintain a characteristic psychological profile but one that is distinct for males and females."}
{"id": "PMID:485812", "title": "Primal-scene experiences: quantitative assessment of an interview study.", "content": "Data are reported from detailed semistructured interviews with 25 males and 25 females, 18-30 years of age. Special attention is given to age and reactions at time of initial awareness of parents' sexual relationship and at time of any possible primal-scene witnessing, beliefs about parents' present sex lives, and interviewees' own ideas of how their parents' sexual attitudes and relationship may have influenced them. Particularly interesting are data indicating that primal-scene experiences, including actual witnessing, do not necessarily result in pathological consequences for psychosexual functioning or satisfaction. The importance of family-relationship patterns as determinants of reactions to primal-scene experience is emphasized.", "contents": "Primal-scene experiences: quantitative assessment of an interview study. Data are reported from detailed semistructured interviews with 25 males and 25 females, 18-30 years of age. Special attention is given to age and reactions at time of initial awareness of parents' sexual relationship and at time of any possible primal-scene witnessing, beliefs about parents' present sex lives, and interviewees' own ideas of how their parents' sexual attitudes and relationship may have influenced them. Particularly interesting are data indicating that primal-scene experiences, including actual witnessing, do not necessarily result in pathological consequences for psychosexual functioning or satisfaction. The importance of family-relationship patterns as determinants of reactions to primal-scene experience is emphasized."}
{"id": "PMID:485813", "title": "On the stability of stigmatization: the case of ex-homosexual males.", "content": "We undertook to empirically identify variables that influence the stigmatization of ex-homosexuals by analyzing the relationship between stigmatization and seven variables held to influence negative attitudes toward members of other ex-deviant groups: the stigmatizer's age, highest achieved educational level, amount of interaction the stigmatizer has had with an ex-homosexual, degree of effectiveness attributed to treatment in changing the homosexual to a heterosexual orientation, degree of responsibility that the stigmatizer attributes to ex-homosexuals for their prior homosexual behavior, degree of dangerousness attributed to ex-homosexuals, and degree of seriousness attributed to homosexuality. Analysis of data obtained by questionnaire from 281 respondents showed that the variable most strongly related to stigmatization of ex-homosexuals was the degree of dangerousness attributed to them. There was a profound direct relationship between these variables. Further, educational level, belief in the effectiveness of treatment, and amount of interaction of ex-homosexuals were inversely related to stigmatization, while degree of seriousness attributed to homosexuality was directly related to stigmatization. Respondents' tendency to stigmatize was not found to be related to age or to their attribution of responsibility to the ex-homosexual for his previous sexual orientation.", "contents": "On the stability of stigmatization: the case of ex-homosexual males. We undertook to empirically identify variables that influence the stigmatization of ex-homosexuals by analyzing the relationship between stigmatization and seven variables held to influence negative attitudes toward members of other ex-deviant groups: the stigmatizer's age, highest achieved educational level, amount of interaction the stigmatizer has had with an ex-homosexual, degree of effectiveness attributed to treatment in changing the homosexual to a heterosexual orientation, degree of responsibility that the stigmatizer attributes to ex-homosexuals for their prior homosexual behavior, degree of dangerousness attributed to ex-homosexuals, and degree of seriousness attributed to homosexuality. Analysis of data obtained by questionnaire from 281 respondents showed that the variable most strongly related to stigmatization of ex-homosexuals was the degree of dangerousness attributed to them. There was a profound direct relationship between these variables. Further, educational level, belief in the effectiveness of treatment, and amount of interaction of ex-homosexuals were inversely related to stigmatization, while degree of seriousness attributed to homosexuality was directly related to stigmatization. Respondents' tendency to stigmatize was not found to be related to age or to their attribution of responsibility to the ex-homosexual for his previous sexual orientation."}
{"id": "PMID:485814", "title": "Color as a variable in making an erotic film more arousing.", "content": "Eight adult male subjects participated in a counterbalanced, repeated-measures design to determine the relative efficacy of color and black-and-white visual sexual stimuli in eliciting sexual arousing, as objectively measured by a mercury-in-rubber strain gauge transducer. There were no consistent or significant differences in either the intensity of the pattern of penile responding during color and black-and-white presentations of the same film.", "contents": "Color as a variable in making an erotic film more arousing. Eight adult male subjects participated in a counterbalanced, repeated-measures design to determine the relative efficacy of color and black-and-white visual sexual stimuli in eliciting sexual arousing, as objectively measured by a mercury-in-rubber strain gauge transducer. There were no consistent or significant differences in either the intensity of the pattern of penile responding during color and black-and-white presentations of the same film."}
{"id": "PMID:485815", "title": "Sexual stereotyping of black males in interracial sex.", "content": "Beliefs about black males' sexuality are pervasive in this society, but little studied. This investigation examined white subjects' perceptions of black and white characters in sexually explicit stories, some of which depicted interracial pairings. The perceptions were then examined in relationship to authoritarianism and sexual repression. Overall results suggest that black males are stereotyped as more potent than white males. The theoretical implications of this finding are discussed.", "contents": "Sexual stereotyping of black males in interracial sex. Beliefs about black males' sexuality are pervasive in this society, but little studied. This investigation examined white subjects' perceptions of black and white characters in sexually explicit stories, some of which depicted interracial pairings. The perceptions were then examined in relationship to authoritarianism and sexual repression. Overall results suggest that black males are stereotyped as more potent than white males. The theoretical implications of this finding are discussed."}
{"id": "PMID:485816", "title": "Castration for sex offenders: treatment or punishment? A review and critique of recent European literature.", "content": "The recent European literature on surgical castration in treatment of sex offenders is reviewed. Results are reported of the most important empirical studies conducted in this field of sex research in Germany, Switzerland, Norway, and Denmark. Methodological problems of follow-up studies on castrates as well as the subject of castration as treatment for sex offenders as a whole are discussed. The main conclusion is that there is no scientific or ethical basis for castration in the treatment of sex offenders.", "contents": "Castration for sex offenders: treatment or punishment? A review and critique of recent European literature. The recent European literature on surgical castration in treatment of sex offenders is reviewed. Results are reported of the most important empirical studies conducted in this field of sex research in Germany, Switzerland, Norway, and Denmark. Methodological problems of follow-up studies on castrates as well as the subject of castration as treatment for sex offenders as a whole are discussed. The main conclusion is that there is no scientific or ethical basis for castration in the treatment of sex offenders."}
{"id": "PMID:485819", "title": "'Malignant' diverticulitis: a clinical entity.", "content": "A form of severe diverticulitis exists that is characterized by (1) phlegmonous inflammation of the sigmoid and rectosigmoid colon, often extending below the peritoneal reflection, (2) frequent fistulization to skin, urinary bladder, and small intestine, (3) frequent colonic obstruction, and (4) high postoperative morbidity and mortality. The clinical similarity to granulomatous colitis is very apparent, but pathologic findings are consistent with severe diverticulitis rather than granulomatous colitis. The term \"malignant\" diverticulitis is suggested because of the progressive nature of the disease process and the frequency of severe morbidity associated with it. Seventeen patients have been studied since 1965, comprising approximately 7% of patients operated on for diverticular disease at Cedars-Sinai Medical Center.", "contents": "'Malignant' diverticulitis: a clinical entity. A form of severe diverticulitis exists that is characterized by (1) phlegmonous inflammation of the sigmoid and rectosigmoid colon, often extending below the peritoneal reflection, (2) frequent fistulization to skin, urinary bladder, and small intestine, (3) frequent colonic obstruction, and (4) high postoperative morbidity and mortality. The clinical similarity to granulomatous colitis is very apparent, but pathologic findings are consistent with severe diverticulitis rather than granulomatous colitis. The term \"malignant\" diverticulitis is suggested because of the progressive nature of the disease process and the frequency of severe morbidity associated with it. Seventeen patients have been studied since 1965, comprising approximately 7% of patients operated on for diverticular disease at Cedars-Sinai Medical Center."}
{"id": "PMID:485820", "title": "Congenital diaphragmatic hernias: eleven years' experience.", "content": "Fifty-five patients with congenital diaphragmatic hernias (1966 to 1976) were studied and compared to 65 similar patients reported previously (1953 to 1963). The mortality was found to be the same in the two groups despite the fact that in the current series, patients were diagnosed and operated on at a younger age. Initial uncorrected pH value was found to be of prognostic importance. All babies whose initial uncorrected pH was greater than 7.0 survived and almost all those whose pH was less than 7.0 died, with the group in-between having a 50% chance of survival. Earlier operation and correction of acidosis did not substantially improve the chance of survival. Hypoplasia of the lungs and major cardiovascular anomalies contributed to death in a number of patients. Pulmonary hypertension causing a right-to-left shunt was responsible for the deaths of others and, therefore, the use of vasodilator drugs deserves further evaluation.", "contents": "Congenital diaphragmatic hernias: eleven years' experience. Fifty-five patients with congenital diaphragmatic hernias (1966 to 1976) were studied and compared to 65 similar patients reported previously (1953 to 1963). The mortality was found to be the same in the two groups despite the fact that in the current series, patients were diagnosed and operated on at a younger age. Initial uncorrected pH value was found to be of prognostic importance. All babies whose initial uncorrected pH was greater than 7.0 survived and almost all those whose pH was less than 7.0 died, with the group in-between having a 50% chance of survival. Earlier operation and correction of acidosis did not substantially improve the chance of survival. Hypoplasia of the lungs and major cardiovascular anomalies contributed to death in a number of patients. Pulmonary hypertension causing a right-to-left shunt was responsible for the deaths of others and, therefore, the use of vasodilator drugs deserves further evaluation."}
{"id": "PMID:485821", "title": "Gastrointestinal anomalies associated with esophageal atresia or tracheoesophageal fistula.", "content": "A retrospective analysis of 150 consecutive patients with esophageal atresia and/or tracheoesophageal fistula was undertaken. The incidence and variety of gastrointestinal (GI) anomalies, the method of management, and the cause of death were critically reviewed to outline preferred methods of management. Of these patients, 87 were male and 63 female. There were 62 GI anomalies in 40 patients. The most common GI anomalies included anorectal anomalies in 17 patients, malrotation of the midgut in 13 patients, duodenal atresia or stenosis in ten patients, and annular pancreas and ectopic pancreatic tissue in three patients each. Six patients had the combination of tracheoesophageal fistula, duodenal obstruction, and anorectal anomaly. Of the 150 patients, 23 have died, with 15 deaths occurring in those with associated GI anomalies. The GI anomalies contributed directly to the deaths of five of these 15 patients. Delay in diagnosis and, on occasion, improper initial management resulted in increased morbidity and mortality.", "contents": "Gastrointestinal anomalies associated with esophageal atresia or tracheoesophageal fistula. A retrospective analysis of 150 consecutive patients with esophageal atresia and/or tracheoesophageal fistula was undertaken. The incidence and variety of gastrointestinal (GI) anomalies, the method of management, and the cause of death were critically reviewed to outline preferred methods of management. Of these patients, 87 were male and 63 female. There were 62 GI anomalies in 40 patients. The most common GI anomalies included anorectal anomalies in 17 patients, malrotation of the midgut in 13 patients, duodenal atresia or stenosis in ten patients, and annular pancreas and ectopic pancreatic tissue in three patients each. Six patients had the combination of tracheoesophageal fistula, duodenal obstruction, and anorectal anomaly. Of the 150 patients, 23 have died, with 15 deaths occurring in those with associated GI anomalies. The GI anomalies contributed directly to the deaths of five of these 15 patients. Delay in diagnosis and, on occasion, improper initial management resulted in increased morbidity and mortality."}
{"id": "PMID:485822", "title": "Surgical management of congenital lymphedema in infants and children.", "content": "Of 67 children and infants with lymphedema, 28 had the congenital type. Congenital lymphedema appears during the first few weeks of life, frequently involves more than one extremity, and enlarges at a slower rate than general body growth. The swelling usually becomes less pronounced with age, and no specific therapy is required in two thirds of the patients. Seven of the 28 children had swelling of the upper extremities and a generalized lymphangiopathy syndrome. Subcutaneous lymphangiectomy was performed on ten of 28 patients who had moderate to severe swelling. Those with hand and arm involvement were particularly benefited; however, operations on the dorsum of the foot produced hypertrophic scars in one third of the cases. The operation is deferred until after age 2 years to permit optimal technical repair and to identify those patients whose conditions will improve spontaneously.", "contents": "Surgical management of congenital lymphedema in infants and children. Of 67 children and infants with lymphedema, 28 had the congenital type. Congenital lymphedema appears during the first few weeks of life, frequently involves more than one extremity, and enlarges at a slower rate than general body growth. The swelling usually becomes less pronounced with age, and no specific therapy is required in two thirds of the patients. Seven of the 28 children had swelling of the upper extremities and a generalized lymphangiopathy syndrome. Subcutaneous lymphangiectomy was performed on ten of 28 patients who had moderate to severe swelling. Those with hand and arm involvement were particularly benefited; however, operations on the dorsum of the foot produced hypertrophic scars in one third of the cases. The operation is deferred until after age 2 years to permit optimal technical repair and to identify those patients whose conditions will improve spontaneously."}
{"id": "PMID:485823", "title": "Carotid bruit: a follow-up report on its significance in patients undergoing an abdominal aortic operation.", "content": "The records of 516 patients who underwent an elective abdominal aortic operation for aneurysmal or occlusive disease are reviewed. Four patients operated on twice are considered an individual patient for each operation. Excluding 27 patients who had a prior carotid endarterectomy, there were 84 with a carotid bruit. Thirty-six patients without a carotid bruit had experienced an episode of cerebral ischemia. Four patients had a postoperative stroke. There was no correlation between the stroke and presence of a carotid bruit or history of cerebral ischemic symptoms. We do not challenge the validity of prophylactic carotid endarterectomy but question if the need for an abdominal aortic operation should be considered indication for carotid angiography and surgery in patients with a carotid bruit.", "contents": "Carotid bruit: a follow-up report on its significance in patients undergoing an abdominal aortic operation. The records of 516 patients who underwent an elective abdominal aortic operation for aneurysmal or occlusive disease are reviewed. Four patients operated on twice are considered an individual patient for each operation. Excluding 27 patients who had a prior carotid endarterectomy, there were 84 with a carotid bruit. Thirty-six patients without a carotid bruit had experienced an episode of cerebral ischemia. Four patients had a postoperative stroke. There was no correlation between the stroke and presence of a carotid bruit or history of cerebral ischemic symptoms. We do not challenge the validity of prophylactic carotid endarterectomy but question if the need for an abdominal aortic operation should be considered indication for carotid angiography and surgery in patients with a carotid bruit."}
{"id": "PMID:485824", "title": "Surgical safaris with the East Africa Flying Doctor Service.", "content": "A few hundred small mission and government hospitals serve the 20 million people who live in rural East Africa. The physician-patient ratio is 1:25,000. Surgical support to many of these hospitals is provided through the East Africa Flying Doctor Service of the African Medical and Research Foundation. Small aircraft that are coordinated by a wide radio network bring surgical capability to a maximum number of patients in this area of 1,300,000 sq km (500,00 sq miles). The clinical problems, differential diagnoses, operative decisions, risk, and complications of surgical care all have a distinctly African quality. The working conditions and variety and magnitude of problems provide a stimulating challenge. Surgical safaris are a catalyst for gaining acceptance of modern scientific methods by Africa's tradition-oriented tribesmen.", "contents": "Surgical safaris with the East Africa Flying Doctor Service. A few hundred small mission and government hospitals serve the 20 million people who live in rural East Africa. The physician-patient ratio is 1:25,000. Surgical support to many of these hospitals is provided through the East Africa Flying Doctor Service of the African Medical and Research Foundation. Small aircraft that are coordinated by a wide radio network bring surgical capability to a maximum number of patients in this area of 1,300,000 sq km (500,00 sq miles). The clinical problems, differential diagnoses, operative decisions, risk, and complications of surgical care all have a distinctly African quality. The working conditions and variety and magnitude of problems provide a stimulating challenge. Surgical safaris are a catalyst for gaining acceptance of modern scientific methods by Africa's tradition-oriented tribesmen."}
{"id": "PMID:485825", "title": "'Kissing' duodenal ulcers.", "content": "Among 70 cases of perforated duodenal ulcers treated by plication, eight were complicated by massive postoperative hemorrhage from a syncronous posterior \"kissing\" duodenal ulcer. Critical analysis revealed that only signs of gastrointestinal (GI) bleeding preoperatively had predictive value for postoperative hemorrhage. Twenty-four patients had one or more predictive signs, and eight actually bled postoperatively. There was a 50% mortality and 75% additional complication rate for the bleeders. In contrast, the nonbleeders had a mortality and a complication rate of only 18% and 35%, respectively. There was no observed superiority of either surgical or medical treatment for postoperative hemorrhage. In perforated duodenal ulcer with evidence of GI blood loss, an intraoperative search for a posterior kissing ulcer is recommended. If a kissing ulcer is found, an acid-reducing operation and suture ligation of the ulcer is indicated.", "contents": "'Kissing' duodenal ulcers. Among 70 cases of perforated duodenal ulcers treated by plication, eight were complicated by massive postoperative hemorrhage from a syncronous posterior \"kissing\" duodenal ulcer. Critical analysis revealed that only signs of gastrointestinal (GI) bleeding preoperatively had predictive value for postoperative hemorrhage. Twenty-four patients had one or more predictive signs, and eight actually bled postoperatively. There was a 50% mortality and 75% additional complication rate for the bleeders. In contrast, the nonbleeders had a mortality and a complication rate of only 18% and 35%, respectively. There was no observed superiority of either surgical or medical treatment for postoperative hemorrhage. In perforated duodenal ulcer with evidence of GI blood loss, an intraoperative search for a posterior kissing ulcer is recommended. If a kissing ulcer is found, an acid-reducing operation and suture ligation of the ulcer is indicated."}
{"id": "PMID:485826", "title": "Selective management of subphrenic abscesses.", "content": "Although extraserous drainage of subphrenic abscesses has gained wide acceptance, there is some renewed enthusiasm for the more frequent use of a transperitoneal operation because it affords the opportunity to discover unsuspected pathologic conditions, particularly heterotopic abscess. In 44 patients with postoperative subphrenic abscesses, the approach to drainage was selected on the basis of the clinical circumstances. Among 28 patients whose abscesses were drained extraserously, the incidence of heteroptic and recurrent abscesses was low. No serious complications of peritoneal or wound soilage occurred after transperitoneal drainage in 16 patients, yet the problems of inadequate drainage and heteroptic abscess were not eliminated. Celiotomy prior to definitive abscess localization was required for 13 patients. Five patients died. The operative approach should be based on the clinical assessment of the patient and particularly on the probability that multicentric intra-abdominal pathologic conditions exist.", "contents": "Selective management of subphrenic abscesses. Although extraserous drainage of subphrenic abscesses has gained wide acceptance, there is some renewed enthusiasm for the more frequent use of a transperitoneal operation because it affords the opportunity to discover unsuspected pathologic conditions, particularly heterotopic abscess. In 44 patients with postoperative subphrenic abscesses, the approach to drainage was selected on the basis of the clinical circumstances. Among 28 patients whose abscesses were drained extraserously, the incidence of heteroptic and recurrent abscesses was low. No serious complications of peritoneal or wound soilage occurred after transperitoneal drainage in 16 patients, yet the problems of inadequate drainage and heteroptic abscess were not eliminated. Celiotomy prior to definitive abscess localization was required for 13 patients. Five patients died. The operative approach should be based on the clinical assessment of the patient and particularly on the probability that multicentric intra-abdominal pathologic conditions exist."}
{"id": "PMID:485827", "title": "Cholate sodium infusion for retained common bile duct stones.", "content": "Eight patients with stones retained in the extrahepatic biliary tract underwent cholate sodium infusion for dissolution of the stones. In six patients, the stones disappeared. However, in two of the patients, the stones did not disappear, and they were removed with the ureteral basket. Infusion of cholate sodium was well tolerated by six patients, but was accompanied by nausea, vomiting, and abdominal pain in two patients. In one case, mucosal deformity of the common bile duct was noticed during the infusion, but it was not apparent on a subsequent cholangiogram. As a result of our experience, we conclude that cholate sodium infusion is a safe procedure for the attempted dissolution of retained common bile duct stones.", "contents": "Cholate sodium infusion for retained common bile duct stones. Eight patients with stones retained in the extrahepatic biliary tract underwent cholate sodium infusion for dissolution of the stones. In six patients, the stones disappeared. However, in two of the patients, the stones did not disappear, and they were removed with the ureteral basket. Infusion of cholate sodium was well tolerated by six patients, but was accompanied by nausea, vomiting, and abdominal pain in two patients. In one case, mucosal deformity of the common bile duct was noticed during the infusion, but it was not apparent on a subsequent cholangiogram. As a result of our experience, we conclude that cholate sodium infusion is a safe procedure for the attempted dissolution of retained common bile duct stones."}
{"id": "PMID:485828", "title": "Raynaud's phenomenon: primary and secondary causes.", "content": "The terms \"Raynaud's disease\" and \"Raynaud's phenomenon\" are often used interchangeably, as if they were a single problem. Because prognosis and therapy are different with each of these entities, 86 patients with episodic digit ischemia were evaluated over the past five years. All patients were evaluated similarly with plethysmography, angiography, and other studies for underlying causes of the digital ischemia. From the study, it was concluded that the majority of the patients had an underlying cause for the problem even though they satisfied the commonly accepted criteria for the diagnosis of Raynaud's disease. It is clear that the diagnosis of Raynaud's disease must be one of exclusion, because it is more uncommon than is generally appreciated by the medical community.", "contents": "Raynaud's phenomenon: primary and secondary causes. The terms \"Raynaud's disease\" and \"Raynaud's phenomenon\" are often used interchangeably, as if they were a single problem. Because prognosis and therapy are different with each of these entities, 86 patients with episodic digit ischemia were evaluated over the past five years. All patients were evaluated similarly with plethysmography, angiography, and other studies for underlying causes of the digital ischemia. From the study, it was concluded that the majority of the patients had an underlying cause for the problem even though they satisfied the commonly accepted criteria for the diagnosis of Raynaud's disease. It is clear that the diagnosis of Raynaud's disease must be one of exclusion, because it is more uncommon than is generally appreciated by the medical community."}
{"id": "PMID:485829", "title": "Duodenal ulcer: its treatment by parietal cell vagotomy at the time of cardiovascular operation.", "content": "The existence of duodenal ulcer disease constitutes a potential threat during the recovery that follows any major operative procedure. At the time of cardiovascular operations, it would be desirable to treat the coexisting duodenal ulcer disease with a definitive ulcer operation if it could be done without entering the gastrointestinal (GI) tract, thereby eliminating the risk of graft contamination. Parietal cell vagotomy with pyloroplasty meets these conditions. During an 18-month period, 309 patients underwent cardiovascular procedures; ten underwent parietal cell vagotomy at the same time for treatment of duodenal ulcer. None of the patients experienced GI complications or the development of any other operative complications referable to the gastric procedure. Parietal cell vagotomy for active duodenal ulcer disease seems to be eminently suited as a companion for cardiovascular procedures that must be expedited.", "contents": "Duodenal ulcer: its treatment by parietal cell vagotomy at the time of cardiovascular operation. The existence of duodenal ulcer disease constitutes a potential threat during the recovery that follows any major operative procedure. At the time of cardiovascular operations, it would be desirable to treat the coexisting duodenal ulcer disease with a definitive ulcer operation if it could be done without entering the gastrointestinal (GI) tract, thereby eliminating the risk of graft contamination. Parietal cell vagotomy with pyloroplasty meets these conditions. During an 18-month period, 309 patients underwent cardiovascular procedures; ten underwent parietal cell vagotomy at the same time for treatment of duodenal ulcer. None of the patients experienced GI complications or the development of any other operative complications referable to the gastric procedure. Parietal cell vagotomy for active duodenal ulcer disease seems to be eminently suited as a companion for cardiovascular procedures that must be expedited."}
{"id": "PMID:485830", "title": "Pancreatitis associated with thiazide administration. A role for the parathyroid glands?", "content": "Twenty-one of a total of 72 patients with acute pancreatitis admitted to a university hospital over a three-year period were found to have \"idiopathic\" pancreatitis. Of these, six nonalcoholic patients without gallbladder disease were receiving one of the thiazide diuretics prior to the onset of pancreatitis. Three patients taken from an earlier series likewise had pancreatitis associated with thiazide administration and at the time of autopsy harbored parathyroid hyperplasia. It is suggested that both the parathyroids and the pancreas may be affected by thiazide administration, and that a history of ingestion of these drugs should be sought in patients who have idiopathic pancreatitis.", "contents": "Pancreatitis associated with thiazide administration. A role for the parathyroid glands? Twenty-one of a total of 72 patients with acute pancreatitis admitted to a university hospital over a three-year period were found to have \"idiopathic\" pancreatitis. Of these, six nonalcoholic patients without gallbladder disease were receiving one of the thiazide diuretics prior to the onset of pancreatitis. Three patients taken from an earlier series likewise had pancreatitis associated with thiazide administration and at the time of autopsy harbored parathyroid hyperplasia. It is suggested that both the parathyroids and the pancreas may be affected by thiazide administration, and that a history of ingestion of these drugs should be sought in patients who have idiopathic pancreatitis."}
{"id": "PMID:485831", "title": "Variable manifestations of vascular injury during lumbar disk surgery.", "content": "Major vascular injury is an unusual but well-recognized complication of lumbar disk surgery. Clinical manifestation of such injuries may be extremely variable. Isolated arterial laceration is most common, with early manifestation due to retroperitoneal hemorrhage. There are often few external signs of blood loss, however, and the diagnosis may not be recognized initially. Formation of an arteriovenous fistula or false aneurysm produces even fewer early signs, and diagnosis is often delayed for weeks or years. Six cases are described that illustrate the full spectrum of acute and chronic manifestations of such injuries. Two cases of acute hemorrhage due to arterial trauma were seen; one case was recognized intraoperatively and one in the recovery room. In four cases arteriovenous fistulae developed and were diagnosed from eight hours to eight years postoperatively. Two cases also had associated false aneurysms, one the probable source of pulmonary emboli and one the principal manifestation of the vascular injury.", "contents": "Variable manifestations of vascular injury during lumbar disk surgery. Major vascular injury is an unusual but well-recognized complication of lumbar disk surgery. Clinical manifestation of such injuries may be extremely variable. Isolated arterial laceration is most common, with early manifestation due to retroperitoneal hemorrhage. There are often few external signs of blood loss, however, and the diagnosis may not be recognized initially. Formation of an arteriovenous fistula or false aneurysm produces even fewer early signs, and diagnosis is often delayed for weeks or years. Six cases are described that illustrate the full spectrum of acute and chronic manifestations of such injuries. Two cases of acute hemorrhage due to arterial trauma were seen; one case was recognized intraoperatively and one in the recovery room. In four cases arteriovenous fistulae developed and were diagnosed from eight hours to eight years postoperatively. Two cases also had associated false aneurysms, one the probable source of pulmonary emboli and one the principal manifestation of the vascular injury."}
{"id": "PMID:485832", "title": "Obturator canal bypass grafts for septic lesions of the femoral artery.", "content": "Infection of the femoral artery results in false aneurysm formation and hemorrhage unless appropriate reconstructive measures are taken. We reviewed ten such cases managed by obturator canal bypass with autogenous saphenous vein. The graft maintained viability of the extremity in every case in the early postoperative period. One death occurred related to cardiac disease, and there was one late failure. This experience confirms the usefulness of the obturator canal as a method for bypass of the infected femoral artery.", "contents": "Obturator canal bypass grafts for septic lesions of the femoral artery. Infection of the femoral artery results in false aneurysm formation and hemorrhage unless appropriate reconstructive measures are taken. We reviewed ten such cases managed by obturator canal bypass with autogenous saphenous vein. The graft maintained viability of the extremity in every case in the early postoperative period. One death occurred related to cardiac disease, and there was one late failure. This experience confirms the usefulness of the obturator canal as a method for bypass of the infected femoral artery."}
{"id": "PMID:485833", "title": "Predicting success of forefoot amputations in diabetics by noninvasive testing.", "content": "Sixty-six diabetic patients underwent needed forefoot amputations when clinical assessment indicated a reasonable chance of healing. All patients underwent noninvasive testing consisting of segmental systolic pressure measurements and pulse volume recordings (PVRs) taken at the thigh, calf, ankle, and forefoot levels. Segmental systolic pressures were falsely high (greater than 200 mm Hg) and therefore not useful in 56%. Ankle systolic pressures predicted failure in 36% of patients who healed and success in 64% who failed to heal. Segmental PVRs were sequentially predictive in only 50%. Forefoot PVR traces predicted failure in 50% of patients whose amputations healed. No patient should be denied a forefoot amputation solely on the basis of unfavorable results of noninvasive tests. Favorable clinical signs and a strongly positive forefoot PVR trace are the best predictors of successful forefoot amputations in diabetic patients.", "contents": "Predicting success of forefoot amputations in diabetics by noninvasive testing. Sixty-six diabetic patients underwent needed forefoot amputations when clinical assessment indicated a reasonable chance of healing. All patients underwent noninvasive testing consisting of segmental systolic pressure measurements and pulse volume recordings (PVRs) taken at the thigh, calf, ankle, and forefoot levels. Segmental systolic pressures were falsely high (greater than 200 mm Hg) and therefore not useful in 56%. Ankle systolic pressures predicted failure in 36% of patients who healed and success in 64% who failed to heal. Segmental PVRs were sequentially predictive in only 50%. Forefoot PVR traces predicted failure in 50% of patients whose amputations healed. No patient should be denied a forefoot amputation solely on the basis of unfavorable results of noninvasive tests. Favorable clinical signs and a strongly positive forefoot PVR trace are the best predictors of successful forefoot amputations in diabetic patients."}
{"id": "PMID:485834", "title": "Prophylactic interruption of the inferior vena cava: immediate and long-term hemodynamic effects.", "content": "Two hundred patients were evaluated retrospectively to determine the clinical effects of prophylactic inferior vena cava (IVC) interruption in association with aortic reconstruction. No pulmonary embolism occurred in the group with IVC interruption, but embolisms did occur in seven of 68 patients who had aortic reconstruction performed without IVC interruption. In two patients, the pulmonary embolism was fatal. Postoperative incidence of deep vein thrombosis was fatal. Postoperative incidence of deep vein thrombosis was 9% in both groups. Clinical and hemodynamic effects of prophylactic IVC interruption were studied in 20 additional patients. Venous hemodynamics (maximum venous outflow, inferior vena cava pressure, and ambulatory venous pressure) showed no change following interruption in 19/20. Sixteen patients from the original group of patients with prophylactic interruption were studied hemodyamically. No pulmonary embolism was clinically evident. One new case of deep vein thrombosis was seen. Again, venous hemodynamics showed no change as a result of IVC interruption. Prophylactic IVC interruption is a safe means of decreasing the incidence of pulmonary embolism without increasing venous-related morbidity.", "contents": "Prophylactic interruption of the inferior vena cava: immediate and long-term hemodynamic effects. Two hundred patients were evaluated retrospectively to determine the clinical effects of prophylactic inferior vena cava (IVC) interruption in association with aortic reconstruction. No pulmonary embolism occurred in the group with IVC interruption, but embolisms did occur in seven of 68 patients who had aortic reconstruction performed without IVC interruption. In two patients, the pulmonary embolism was fatal. Postoperative incidence of deep vein thrombosis was fatal. Postoperative incidence of deep vein thrombosis was 9% in both groups. Clinical and hemodynamic effects of prophylactic IVC interruption were studied in 20 additional patients. Venous hemodynamics (maximum venous outflow, inferior vena cava pressure, and ambulatory venous pressure) showed no change following interruption in 19/20. Sixteen patients from the original group of patients with prophylactic interruption were studied hemodyamically. No pulmonary embolism was clinically evident. One new case of deep vein thrombosis was seen. Again, venous hemodynamics showed no change as a result of IVC interruption. Prophylactic IVC interruption is a safe means of decreasing the incidence of pulmonary embolism without increasing venous-related morbidity."}
{"id": "PMID:485835", "title": "Positive blood culture as an aid in the diagnosis of secondary aortoenteric fistula.", "content": "The successful management of aortoenteric fistula (AEF) requires early diagnosis. To evaluate the accuracy of our diagnostic approach, the hospital course of seven patients with AEF was reviewed. In six patients, the initial bleeding episodes were of the low volume type characterized by hematemesis and melena or by melena alone. All patients were febrile. In six patients, blood cultures obtained preoperatively were positive for enteric organisms identical to those found in cultures obtained intraoperatively from the AEF site. Roentgenographic examination of the upper gastrointestinal (GI) tract performed in three patients was diagnostic for AEF in only one. Endoscopy in seven patients revealed a bleeding suture line in one. Angiography was not diagnostic in the six patients in whom it was performed. When patients are seen with the triad of GI bleeding, a history of aortic surgery, and fever, aerobic and anaerobic blood cultures should be obtained. If blood cultures are positive for enteric organisms, the diagnosis of AEF should be strongly suspected, and early surgical intervention is indicated.", "contents": "Positive blood culture as an aid in the diagnosis of secondary aortoenteric fistula. The successful management of aortoenteric fistula (AEF) requires early diagnosis. To evaluate the accuracy of our diagnostic approach, the hospital course of seven patients with AEF was reviewed. In six patients, the initial bleeding episodes were of the low volume type characterized by hematemesis and melena or by melena alone. All patients were febrile. In six patients, blood cultures obtained preoperatively were positive for enteric organisms identical to those found in cultures obtained intraoperatively from the AEF site. Roentgenographic examination of the upper gastrointestinal (GI) tract performed in three patients was diagnostic for AEF in only one. Endoscopy in seven patients revealed a bleeding suture line in one. Angiography was not diagnostic in the six patients in whom it was performed. When patients are seen with the triad of GI bleeding, a history of aortic surgery, and fever, aerobic and anaerobic blood cultures should be obtained. If blood cultures are positive for enteric organisms, the diagnosis of AEF should be strongly suspected, and early surgical intervention is indicated."}
{"id": "PMID:485836", "title": "Limitations of noninvasive evaluation of carotid occlusive disease.", "content": "A series of 32 patients examined by two different noninvasive techniques in two different laboratories and by cerebral angiography were retrospectively studied. Significant differences in ophthalmic artery pressures as measured by oculopneumoplethysmography (OPPG), and significant delays in ocular pulse arrival time combined with characteristic recorded bruits as determined by oculoplethysmography/carotid phonoangiography (OPG/CPA) were considered as an index of carotid stenosis. Cerebral angiography was performed on all 32 patients to confirm or refute the diagnosis suggested by noninvasive testing. In this selected group of patients with difficult diagnostic problems, only 67% of significant (greater than 50% diameter) stenoses were detected by OPPG, and only 58% by OPG/CPA. Combining OPPG and OPG/CPA increased the probability of detecting a greater than 50% diameter stenosis to 92%.", "contents": "Limitations of noninvasive evaluation of carotid occlusive disease. A series of 32 patients examined by two different noninvasive techniques in two different laboratories and by cerebral angiography were retrospectively studied. Significant differences in ophthalmic artery pressures as measured by oculopneumoplethysmography (OPPG), and significant delays in ocular pulse arrival time combined with characteristic recorded bruits as determined by oculoplethysmography/carotid phonoangiography (OPG/CPA) were considered as an index of carotid stenosis. Cerebral angiography was performed on all 32 patients to confirm or refute the diagnosis suggested by noninvasive testing. In this selected group of patients with difficult diagnostic problems, only 67% of significant (greater than 50% diameter) stenoses were detected by OPPG, and only 58% by OPG/CPA. Combining OPPG and OPG/CPA increased the probability of detecting a greater than 50% diameter stenosis to 92%."}
{"id": "PMID:485837", "title": "Simplified splanchnic artery revascularization using extra-anatomic bypass grafts: a report of ten cases.", "content": "Revascularization of the superior mesenteric artery or renal artery is frequently complicated by concomitant atherosclerotic disease in the adjacent aorta. In ten patients, extra-anatomic saphenous vein bypass grafts to the splanchnic vessels were constructed and inflow was obtained from the external iliac artery. All patients recovered without complication and have had functioning grafts on follow-up arteriography. This extra-anatomic bypass provides a simple method for splanchnic artery revascularization.", "contents": "Simplified splanchnic artery revascularization using extra-anatomic bypass grafts: a report of ten cases. Revascularization of the superior mesenteric artery or renal artery is frequently complicated by concomitant atherosclerotic disease in the adjacent aorta. In ten patients, extra-anatomic saphenous vein bypass grafts to the splanchnic vessels were constructed and inflow was obtained from the external iliac artery. All patients recovered without complication and have had functioning grafts on follow-up arteriography. This extra-anatomic bypass provides a simple method for splanchnic artery revascularization."}
{"id": "PMID:485838", "title": "Portal hypertension after bile duct obstruction: effect of bile diversion on portal pressure in the rat.", "content": "Biliary obstruction of 14 and 28 days induced in the rat an increase of portal pressure (PP) and wedge hepatic vein pressure (WHVP); the higher these were, the longer was the obstruction. Occurrence of portal hypertension seemed related to portal and periportal fibrosis. Relief of obstruction after 14 days by bilioduodenal anastomosis brought back to normal PP and WHVP. In rats with longer obstruction periods, bilioduodenal anastomosis failed to lower PP and WHPV although biological signs of cholestasis returned to normal levels. These results suggest that portal hypertension may arise very shortly after biliary obstruction in rats and that it may persist in animals with a prolonged biliary obstruction despite an efficient bile drainage. In clinical conditions, such results would favor early treatment of lesions that usually cause prolonged bile duct obstruction, such as postoperative bile duct stenosis.", "contents": "Portal hypertension after bile duct obstruction: effect of bile diversion on portal pressure in the rat. Biliary obstruction of 14 and 28 days induced in the rat an increase of portal pressure (PP) and wedge hepatic vein pressure (WHVP); the higher these were, the longer was the obstruction. Occurrence of portal hypertension seemed related to portal and periportal fibrosis. Relief of obstruction after 14 days by bilioduodenal anastomosis brought back to normal PP and WHVP. In rats with longer obstruction periods, bilioduodenal anastomosis failed to lower PP and WHPV although biological signs of cholestasis returned to normal levels. These results suggest that portal hypertension may arise very shortly after biliary obstruction in rats and that it may persist in animals with a prolonged biliary obstruction despite an efficient bile drainage. In clinical conditions, such results would favor early treatment of lesions that usually cause prolonged bile duct obstruction, such as postoperative bile duct stenosis."}
{"id": "PMID:485839", "title": "Acute diverticulitis of the transverse colon with perforation: report of three cases and review of the literature.", "content": "Three cases of perforated diverticulitis of the transverse colon are added to 19 case reports in the English literature. This unusual disease entity is usually confused with acute appendicitis prior to operation and with carcinoma of the colon during the procedure. The cause of these diverticula is unclear, and their clinical presentation resembles that of right-sided diverticula. Segmental resection of the transverse colon together with end-to-end anastomosis is the treatment of choice, except in the occasional instance of a large intra-abdominal abscess, when anastomosis may be deferred.", "contents": "Acute diverticulitis of the transverse colon with perforation: report of three cases and review of the literature. Three cases of perforated diverticulitis of the transverse colon are added to 19 case reports in the English literature. This unusual disease entity is usually confused with acute appendicitis prior to operation and with carcinoma of the colon during the procedure. The cause of these diverticula is unclear, and their clinical presentation resembles that of right-sided diverticula. Segmental resection of the transverse colon together with end-to-end anastomosis is the treatment of choice, except in the occasional instance of a large intra-abdominal abscess, when anastomosis may be deferred."}
{"id": "PMID:485840", "title": "Right paraduodenal hernia: a source of chronic abdominal pain in the adult.", "content": "Right paraduodenal hernias are a relatively rare congenital malformation with a total of 50 clinical cases having been reported. This anomaly can be seen as total encapsulation of the small bowel or a single loop within a hernia sac. Symptoms are often chronic vague abdominal pain and intermittent obstructive episodes. Barium studies in patients with moderate and large defects, and angiography in small hernias may provide the diagnosis. The method of repair is dependent on the size of the hernia encountered. The management of two patients with this problem is described.", "contents": "Right paraduodenal hernia: a source of chronic abdominal pain in the adult. Right paraduodenal hernias are a relatively rare congenital malformation with a total of 50 clinical cases having been reported. This anomaly can be seen as total encapsulation of the small bowel or a single loop within a hernia sac. Symptoms are often chronic vague abdominal pain and intermittent obstructive episodes. Barium studies in patients with moderate and large defects, and angiography in small hernias may provide the diagnosis. The method of repair is dependent on the size of the hernia encountered. The management of two patients with this problem is described."}
{"id": "PMID:485841", "title": "Chronic pseudo-obstruction secondary to side-to-side intestinal anastomosis.", "content": "An unusual late complication of side-to-side intestinal anastomosis, chronic small-bowel obstruction with massive proximal ileal dilation despite a widely patent anastomosis, occurred in a patient. The classic blind loop syndrome was not present. Several potential mechanisms are suggested, including regional absence of normal peristalsis on a mechanical basis and bacterial overgrowth. This report adds support to the concept that side-to-side intestinal anastomosis should be avoided whenever possible.", "contents": "Chronic pseudo-obstruction secondary to side-to-side intestinal anastomosis. An unusual late complication of side-to-side intestinal anastomosis, chronic small-bowel obstruction with massive proximal ileal dilation despite a widely patent anastomosis, occurred in a patient. The classic blind loop syndrome was not present. Several potential mechanisms are suggested, including regional absence of normal peristalsis on a mechanical basis and bacterial overgrowth. This report adds support to the concept that side-to-side intestinal anastomosis should be avoided whenever possible."}
{"id": "PMID:485842", "title": "Giant diverticular of the sigmoid colon: a review of differential diagnosis and operative management.", "content": "Giant sigmoid diverticula (\"giant gas cysts,\" \"giant sigmoid cysts\") are an unusual complication of diverticulitis. We have recently seen two patients with such lesions, bringing the total number of reported cases to 48. Most patients are elderly and complain of abdominal pain or the presence of an abdominal mass. Air trapping by a ball-valve mechanism may be responsible for formation of the fibrous cysts. The gas-filled structures can usually be differentiated from other conditions by contrast studies. Excision of the cyst and adjacent colon with anastomosis can usually be accomplished safely under elective circumstances.", "contents": "Giant diverticular of the sigmoid colon: a review of differential diagnosis and operative management. Giant sigmoid diverticula (\"giant gas cysts,\" \"giant sigmoid cysts\") are an unusual complication of diverticulitis. We have recently seen two patients with such lesions, bringing the total number of reported cases to 48. Most patients are elderly and complain of abdominal pain or the presence of an abdominal mass. Air trapping by a ball-valve mechanism may be responsible for formation of the fibrous cysts. The gas-filled structures can usually be differentiated from other conditions by contrast studies. Excision of the cyst and adjacent colon with anastomosis can usually be accomplished safely under elective circumstances."}
{"id": "PMID:485843", "title": "Repair of massive inguinal hernia: with pneumoperitoneum and without using prosthetic mesh.", "content": "There was an unusual need for preoperative induction of an artificial pneumoperitoneum in two cases of inguinal hernia. Both hernias were very large and associated with extensive intertrigo. Daily insufflations of air were performed for 13 and 11 days, with the patients compensating for this increased intraabdominal pressure over this time. Operation with removal of the ipsilateral testicle, cord, and scrotum was then straightforward and convalescence uneventful. The pneumoperitoneum allowed a repair to be effected without using a prosthetic mesh.", "contents": "Repair of massive inguinal hernia: with pneumoperitoneum and without using prosthetic mesh. There was an unusual need for preoperative induction of an artificial pneumoperitoneum in two cases of inguinal hernia. Both hernias were very large and associated with extensive intertrigo. Daily insufflations of air were performed for 13 and 11 days, with the patients compensating for this increased intraabdominal pressure over this time. Operation with removal of the ipsilateral testicle, cord, and scrotum was then straightforward and convalescence uneventful. The pneumoperitoneum allowed a repair to be effected without using a prosthetic mesh."}
{"id": "PMID:485850", "title": "Colonic complications of acute pancreatitis.", "content": "Colonic complications of acute pancreatitis include \"pseudo-obstruction,\" necrosis, hemorrhage, fistula, and ischemic colitis. With the ten cases reported in this article, there are now 75 cases reported in the literature to our knowledge. The fulminating lesions (necrosis and hemorrhage) are usually associated with pancreatic abscess and/or pseudocyst and may occur because of a direct pressure effect with secondary vascular compromise. The lesions are predominant in the transverse colon and at the splenic flexure. Because the risk factor for a colonic complication from pancreatitis is highest in those patients with inflammatory masses in the body and tail of the gland due to colon contiguity, such masses require individualized treatment, including frequent clinical examination with sequential ultrasonography, and probably early surgical intervention.", "contents": "Colonic complications of acute pancreatitis. Colonic complications of acute pancreatitis include \"pseudo-obstruction,\" necrosis, hemorrhage, fistula, and ischemic colitis. With the ten cases reported in this article, there are now 75 cases reported in the literature to our knowledge. The fulminating lesions (necrosis and hemorrhage) are usually associated with pancreatic abscess and/or pseudocyst and may occur because of a direct pressure effect with secondary vascular compromise. The lesions are predominant in the transverse colon and at the splenic flexure. Because the risk factor for a colonic complication from pancreatitis is highest in those patients with inflammatory masses in the body and tail of the gland due to colon contiguity, such masses require individualized treatment, including frequent clinical examination with sequential ultrasonography, and probably early surgical intervention."}
{"id": "PMID:485851", "title": "Investigation of cardiac glycoside levels in human post mortem blood and tissues determined by a special radioimmunoassay procedure.", "content": "Even after the introduction of radioimmunological methods the question of a cardiac glycoside causing or contributing to the death of a patient can not be answered satisfactorily. By means of a special radioimmunoassay procedure for digoxin as well as for the structurally related methyl- and acetylderivatives we measured the concentrations in human blood and post mortem tissues. We investigated the glycoside contents in the blood of intravenously digitalised (Novodigal) al) patients before and after death. At autopsy blood specimens were taken from the heart and the femoral vein. We found an increase of the glycoside level up to a highly toxic range (7--15 ng/ml) especially in the heart blood. Thus post mortem blood levels of digoxin and its derivatives are not suitable for a final decision in alleged cases of fatal poisonings. Measuring various concentrations in tussues and body fluids of the above cardiac glycosides mentioned revealed the kidney concentration to be of high value in confirming a digitalis poisoning. This organ and the heart show the highest tissue concentrations. Interpretations of fatal digitalis poisonings should be based on the additional knowlege of these concentrations. Individual cardiac glycosides may be analyzed by a combination of thin layer chromatography and radioimmunoassay.", "contents": "Investigation of cardiac glycoside levels in human post mortem blood and tissues determined by a special radioimmunoassay procedure. Even after the introduction of radioimmunological methods the question of a cardiac glycoside causing or contributing to the death of a patient can not be answered satisfactorily. By means of a special radioimmunoassay procedure for digoxin as well as for the structurally related methyl- and acetylderivatives we measured the concentrations in human blood and post mortem tissues. We investigated the glycoside contents in the blood of intravenously digitalised (Novodigal) al) patients before and after death. At autopsy blood specimens were taken from the heart and the femoral vein. We found an increase of the glycoside level up to a highly toxic range (7--15 ng/ml) especially in the heart blood. Thus post mortem blood levels of digoxin and its derivatives are not suitable for a final decision in alleged cases of fatal poisonings. Measuring various concentrations in tussues and body fluids of the above cardiac glycosides mentioned revealed the kidney concentration to be of high value in confirming a digitalis poisoning. This organ and the heart show the highest tissue concentrations. Interpretations of fatal digitalis poisonings should be based on the additional knowlege of these concentrations. Individual cardiac glycosides may be analyzed by a combination of thin layer chromatography and radioimmunoassay."}
{"id": "PMID:485852", "title": "Isolation and determination of quaternary ammonium compounds by means of amberlite XAD-columns and thin layer chromatography.", "content": "The potentials of XAD-columns for the isolation of quaternary ammonium compounds from aqueous media have been investigated. When adequate amounts of counter ions (perchlorate, chloride, phosphate, nitrate) were added to the aqueous sample, to the column pretreatment fluid and to the aqueous washing fluid, most quaternary compounds investigated were retained on the column and could be recovered by elution with methanol. This approach proved also suitable for urine. Quantitation of quaternaries isolated in this way from urine samples could be performed on silicagel thin layer plates through visualization with iodine, followed by densitometric evaluation. For decamethonium detection limits were 0.1 micrograms/ml. Recoveries at the 1 micrograms/ml level were between 80--90% with variation coefficients of less than 10%.", "contents": "Isolation and determination of quaternary ammonium compounds by means of amberlite XAD-columns and thin layer chromatography. The potentials of XAD-columns for the isolation of quaternary ammonium compounds from aqueous media have been investigated. When adequate amounts of counter ions (perchlorate, chloride, phosphate, nitrate) were added to the aqueous sample, to the column pretreatment fluid and to the aqueous washing fluid, most quaternary compounds investigated were retained on the column and could be recovered by elution with methanol. This approach proved also suitable for urine. Quantitation of quaternaries isolated in this way from urine samples could be performed on silicagel thin layer plates through visualization with iodine, followed by densitometric evaluation. For decamethonium detection limits were 0.1 micrograms/ml. Recoveries at the 1 micrograms/ml level were between 80--90% with variation coefficients of less than 10%."}
{"id": "PMID:485854", "title": "The effects of 2,2-diethylallylacetamide on hepatic cytochromes in rats and in vitro.", "content": "The application of 0.15 and 0.3% of diethylallylacetamide (DA) in the drinking water to rats during 10 days increased the relative liver weight, the yield of hepatic microsomes, and cytochromes P-450 and b5. Single s.c. doses of 400 mg DA per kg reduced cytochrome P-450 concentrations in the hepatic endoplasmic reticulum of rats. This effect was considerably more pronounced in rats pretreated with phenobarbital. The activity of 5-aminolaevulinic acid synthesis in liver mitochondria, and total liver porphyrins increased. In incubations of metabolizing hepatic microsomes from rabbits pretreated with phenobarbital 75% of 0.1 mM DA was degraded after 1 h. The aerobic incubation of 0.1 mM DA with rabbit hepatic microsomes in the presence of NADPH produced 50% destruction of cytochrome P-450 within 1 h. Addition of EDTA revealed that a part of this destruction cannot be explained by accelerated lipid peroxidation.", "contents": "The effects of 2,2-diethylallylacetamide on hepatic cytochromes in rats and in vitro. The application of 0.15 and 0.3% of diethylallylacetamide (DA) in the drinking water to rats during 10 days increased the relative liver weight, the yield of hepatic microsomes, and cytochromes P-450 and b5. Single s.c. doses of 400 mg DA per kg reduced cytochrome P-450 concentrations in the hepatic endoplasmic reticulum of rats. This effect was considerably more pronounced in rats pretreated with phenobarbital. The activity of 5-aminolaevulinic acid synthesis in liver mitochondria, and total liver porphyrins increased. In incubations of metabolizing hepatic microsomes from rabbits pretreated with phenobarbital 75% of 0.1 mM DA was degraded after 1 h. The aerobic incubation of 0.1 mM DA with rabbit hepatic microsomes in the presence of NADPH produced 50% destruction of cytochrome P-450 within 1 h. Addition of EDTA revealed that a part of this destruction cannot be explained by accelerated lipid peroxidation."}
{"id": "PMID:485856", "title": "Properties of a newly isolated, serologically distinct avian paramyxovirus.", "content": "The morphological, bio-physical and growth properties of the isolate duck/Hong Kong/D3/75 (D3/75) were consistent with this virus being a member of the paramyxovirus group. Using haemagglutination inhibition and neuraminidase inhibition tests no serological relationships between D3/75 and other paramyxoviruses could be demonstrated. The structural polypeptides of D3/75 were also typical of paramyxoviruses, consisting of 6--7 polypeptides ranging in molecular weight from 46,000--200,000 under reduced conditions. Two polypeptides were glycosylated.", "contents": "Properties of a newly isolated, serologically distinct avian paramyxovirus. The morphological, bio-physical and growth properties of the isolate duck/Hong Kong/D3/75 (D3/75) were consistent with this virus being a member of the paramyxovirus group. Using haemagglutination inhibition and neuraminidase inhibition tests no serological relationships between D3/75 and other paramyxoviruses could be demonstrated. The structural polypeptides of D3/75 were also typical of paramyxoviruses, consisting of 6--7 polypeptides ranging in molecular weight from 46,000--200,000 under reduced conditions. Two polypeptides were glycosylated."}
{"id": "PMID:485858", "title": "[Several morphologic changes in the blood vessels of the rabbit medulla oblongata arising under the influence successive exposure to gravitational overloading and hypokinesia].", "content": "Morphological changes in blood vessels of the rabbit medulla oblongata were studied after successive effects of maximum-bearing gravitational overloadings of 10 units applied at different directions and hypokinesia of various duration. Blood system of 35 rabbits was injected with Gerota's mass, cleared horizontal sections of the medulla oblongata 120 mkm thick were stained with hematoxylin--eosin and after Van Gieson. The experiments demonstrated various, quantitative and qualitative, changes in the vessel structure of the medulla oblongata, prevalence of one of the factors applied--in one combination and successive application; total resulting effect of overloading and hypokinesia--in the other combination; peculiar morphological changes unusual to any of the two factors--in the third combination. It was also demonstrated that readaptation for 12 weeks resulted in a significant restoration of the blood vessel structure in the rabbit brains preliminarily subjected to a successive effect of hypokinesia for 4 weeks and overloading in cranio-caudal directions.", "contents": "[Several morphologic changes in the blood vessels of the rabbit medulla oblongata arising under the influence successive exposure to gravitational overloading and hypokinesia]. Morphological changes in blood vessels of the rabbit medulla oblongata were studied after successive effects of maximum-bearing gravitational overloadings of 10 units applied at different directions and hypokinesia of various duration. Blood system of 35 rabbits was injected with Gerota's mass, cleared horizontal sections of the medulla oblongata 120 mkm thick were stained with hematoxylin--eosin and after Van Gieson. The experiments demonstrated various, quantitative and qualitative, changes in the vessel structure of the medulla oblongata, prevalence of one of the factors applied--in one combination and successive application; total resulting effect of overloading and hypokinesia--in the other combination; peculiar morphological changes unusual to any of the two factors--in the third combination. It was also demonstrated that readaptation for 12 weeks resulted in a significant restoration of the blood vessel structure in the rabbit brains preliminarily subjected to a successive effect of hypokinesia for 4 weeks and overloading in cranio-caudal directions."}
{"id": "PMID:485860", "title": "[Age and features of the construction and cellular composition of superior tracheobronchial lymph nodes in adult humans].", "content": "Age peculiarities in composition of the upper tracheobronchial lymph nodes have been studied in 60 corpses at the age of 16-70 years. Cause of death has not been connected with diseases of blood, lymphatic system or thoracic origins. Routine histological and morphometric methods have been applied. Certain age-dependent rearrangement in the connective tissue stroma, trabecular vegetations and differences in correlations of structural components have been noted. A rather essential volumetric increase of the medullary substance takes place at the level of hilus of the lymph node. Cellular composition of the lymph nodes is greatly polymorphous but is more or less constant for each age group. The number of macrophages containing carbon pigment is increasing with age.", "contents": "[Age and features of the construction and cellular composition of superior tracheobronchial lymph nodes in adult humans]. Age peculiarities in composition of the upper tracheobronchial lymph nodes have been studied in 60 corpses at the age of 16-70 years. Cause of death has not been connected with diseases of blood, lymphatic system or thoracic origins. Routine histological and morphometric methods have been applied. Certain age-dependent rearrangement in the connective tissue stroma, trabecular vegetations and differences in correlations of structural components have been noted. A rather essential volumetric increase of the medullary substance takes place at the level of hilus of the lymph node. Cellular composition of the lymph nodes is greatly polymorphous but is more or less constant for each age group. The number of macrophages containing carbon pigment is increasing with age."}
{"id": "PMID:485861", "title": "[Anatomy of lymph nodes situated adjacent to the axilla in adult humans].", "content": "In 50 right and 50 left upper extremities examined in adult persons of both sex at the age of 28-90 years, delto-thoracic lymph nodes were revealed in 30% (right) and in 22% (left), and interthoracic lymph nodes--in 6% (right) and in 12% (left). The lymph nodes in question were revealed by the method of section after interstitial injection of Gerota's blue intradermally to fingers, palm, back of the hand deltoid area, lateral thoracic surface (at the level of the 6th intercostal space) and to the external part of the mammary gland. Injection was also performed into lymphatic vessels revealed by means of the interstitial injection. The delto-thoracic nodes were stated to situate in both the delto-thoracic sulcus and the delto-thoracic triangle. These vessels are situated along the course of the lateral collector of the free upper extremity. Deferent vessels of the delto-thoracic nodes flow into the apical axillary lymph nodes, into the deep and superficial cervical nodes, into the interthoracic lymph nodes and also into the subclavicular or into the jugular vein near a corresponding venous angle. Interthoracic lymph nodes, situated between musculus pectoralis major and minor, get their lymphatic vessels from lateral, inferior and central axillary nodes, from delto-thoracic nodes and also those lymphatic vessels that go from the mammary gland area. Deferent vessels of the interthoracic nodes flow into the apical axillary nodes.", "contents": "[Anatomy of lymph nodes situated adjacent to the axilla in adult humans]. In 50 right and 50 left upper extremities examined in adult persons of both sex at the age of 28-90 years, delto-thoracic lymph nodes were revealed in 30% (right) and in 22% (left), and interthoracic lymph nodes--in 6% (right) and in 12% (left). The lymph nodes in question were revealed by the method of section after interstitial injection of Gerota's blue intradermally to fingers, palm, back of the hand deltoid area, lateral thoracic surface (at the level of the 6th intercostal space) and to the external part of the mammary gland. Injection was also performed into lymphatic vessels revealed by means of the interstitial injection. The delto-thoracic nodes were stated to situate in both the delto-thoracic sulcus and the delto-thoracic triangle. These vessels are situated along the course of the lateral collector of the free upper extremity. Deferent vessels of the delto-thoracic nodes flow into the apical axillary lymph nodes, into the deep and superficial cervical nodes, into the interthoracic lymph nodes and also into the subclavicular or into the jugular vein near a corresponding venous angle. Interthoracic lymph nodes, situated between musculus pectoralis major and minor, get their lymphatic vessels from lateral, inferior and central axillary nodes, from delto-thoracic nodes and also those lymphatic vessels that go from the mammary gland area. Deferent vessels of the interthoracic nodes flow into the apical axillary nodes."}
{"id": "PMID:485862", "title": "[Ultrastructural demonstration of rabbit heart myocyte metabolism during fasting].", "content": "Thirty mature rabbits were completely deprived of food but water ad libitum for 10 days, then half of the animals were decapitated. The remaining animals were sacrificed 10 days after a sound diet. Histological, histochemical (estimation of glycogen, lipids, acid glycosamineglicans) and electron microscopic investigation of the myocardium was carried out. Degenerative and necrobiotic alterations were not revealed in muscular cells of the heart. At fasting accumulation of lipids and glycogen in the cardiac myocytes was evident. In fasting animals ultrastructure of the muscular cells of the heart demonstrated a certain decrease in synthetic processes. After a 10-day sound diet, the cardiac myocytes were actively involved into the process of intracellular regeneration. Increased lipid content in the rabbit myocardium, occurring at fasting, is connected with the necessity to provide the heart with an effective energy substrate required to preserve its contractile function and in this way adaptive-compensatory processes are demonstrated.", "contents": "[Ultrastructural demonstration of rabbit heart myocyte metabolism during fasting]. Thirty mature rabbits were completely deprived of food but water ad libitum for 10 days, then half of the animals were decapitated. The remaining animals were sacrificed 10 days after a sound diet. Histological, histochemical (estimation of glycogen, lipids, acid glycosamineglicans) and electron microscopic investigation of the myocardium was carried out. Degenerative and necrobiotic alterations were not revealed in muscular cells of the heart. At fasting accumulation of lipids and glycogen in the cardiac myocytes was evident. In fasting animals ultrastructure of the muscular cells of the heart demonstrated a certain decrease in synthetic processes. After a 10-day sound diet, the cardiac myocytes were actively involved into the process of intracellular regeneration. Increased lipid content in the rabbit myocardium, occurring at fasting, is connected with the necessity to provide the heart with an effective energy substrate required to preserve its contractile function and in this way adaptive-compensatory processes are demonstrated."}
{"id": "PMID:485863", "title": "[Composium of the histiocyte population of the subcutaneous tissue of white rats (according to the results of a morphometric analysis of their ultrastructure)].", "content": "Investigations on polymorphism of histiocytes (macrophages) in the rat normal loose connective tissue support the suggestion about a complex composition of these cellular population. Two classes of cells (subpopulations K-1 and K-2) separated by means of multidimension morphometric analysis of ultrastructure are well identified by several morphologic criteria of weighed informativity. The cells of both populations are recognized under conditions of the organism's functional loadings supposed to produce macrophage activation. Quantitative rearrangements in the subpopulations composing the histiocyte population and a number of morphological transformations revealed in them as specific for fasting and degeneration proved a real importance of the classification.", "contents": "[Composium of the histiocyte population of the subcutaneous tissue of white rats (according to the results of a morphometric analysis of their ultrastructure)]. Investigations on polymorphism of histiocytes (macrophages) in the rat normal loose connective tissue support the suggestion about a complex composition of these cellular population. Two classes of cells (subpopulations K-1 and K-2) separated by means of multidimension morphometric analysis of ultrastructure are well identified by several morphologic criteria of weighed informativity. The cells of both populations are recognized under conditions of the organism's functional loadings supposed to produce macrophage activation. Quantitative rearrangements in the subpopulations composing the histiocyte population and a number of morphological transformations revealed in them as specific for fasting and degeneration proved a real importance of the classification."}
{"id": "PMID:485864", "title": "[Evolution of the allantoid placenta in placental mammals].", "content": "The article surveys the literature concerning comparative morphology of allantoic placenta and presents some evolutionary-morphological conclusions. The initial form of allantoic placenta is non-deciduate that occurs in 7 orders of placental mammals. The non-deciduate placenta is especially characteristic for lower primates and Cetacea. The non-deciduate placenta in lower primates and the fact that in some modern species of insectivores the non-deciduate placenta is also preserved speaks in favour of the opinion that insectivores of the Cretaceous period had non-deciduate placenta. The non-deciduate placenta in Cetacea that take their origin from ancient carnivores (from procreodonts) demonstrates, in its turn, that the non-deciduate placenta was the initial form. The deciduate placenta was the means for changing many of vital activities of the organism--it is connected with shortening or lengthening of individual life duration and duration of pregnancy. In the course of evolution the allantoic placenta improved towards intensified metabolism between the embryo (fetus) and the maternal organism. Relations between the evolution of mature specimens and evolutional changes in placenta are at their initial stage of investigation.", "contents": "[Evolution of the allantoid placenta in placental mammals]. The article surveys the literature concerning comparative morphology of allantoic placenta and presents some evolutionary-morphological conclusions. The initial form of allantoic placenta is non-deciduate that occurs in 7 orders of placental mammals. The non-deciduate placenta is especially characteristic for lower primates and Cetacea. The non-deciduate placenta in lower primates and the fact that in some modern species of insectivores the non-deciduate placenta is also preserved speaks in favour of the opinion that insectivores of the Cretaceous period had non-deciduate placenta. The non-deciduate placenta in Cetacea that take their origin from ancient carnivores (from procreodonts) demonstrates, in its turn, that the non-deciduate placenta was the initial form. The deciduate placenta was the means for changing many of vital activities of the organism--it is connected with shortening or lengthening of individual life duration and duration of pregnancy. In the course of evolution the allantoic placenta improved towards intensified metabolism between the embryo (fetus) and the maternal organism. Relations between the evolution of mature specimens and evolutional changes in placenta are at their initial stage of investigation."}
{"id": "PMID:485865", "title": "[Ultrastructural informational analysis of the dynamics of liver cell recovery after performance of a biliodigestive anastomosis in mechanical jaundice].", "content": "Hepatic ultrastructure has been studied in dogs at the level of cholestasis and at different time after cholecystenteroanastomosis. Owing to theoretico-informative approach applied in the investigation, it was possible to reveal certain essential disorganization in hepatic ultrastructures on the 7th day after the operation. This phenomenon seems to explain the occurrence of acute hepatic insufficiency at early postoperative stages not infrequently observed in clinic. Changes produced by 30-days' cholestasis are demonstrated to be reversible.", "contents": "[Ultrastructural informational analysis of the dynamics of liver cell recovery after performance of a biliodigestive anastomosis in mechanical jaundice]. Hepatic ultrastructure has been studied in dogs at the level of cholestasis and at different time after cholecystenteroanastomosis. Owing to theoretico-informative approach applied in the investigation, it was possible to reveal certain essential disorganization in hepatic ultrastructures on the 7th day after the operation. This phenomenon seems to explain the occurrence of acute hepatic insufficiency at early postoperative stages not infrequently observed in clinic. Changes produced by 30-days' cholestasis are demonstrated to be reversible."}
{"id": "PMID:485866", "title": "[Dynamics of structural changes in the organs of immunity of rabbits with acute experimental appendicitis].", "content": "Cyto- and histological analysis of the whole immunogenic system at the development of an acute experimental appendicitis in rabbits has demonstrated that all the constituent parts of the system are involved into the reaction. In the central organ of immunity--thymus the main change is its progressive exhaustion, while peripheral organs of immunity are subjected to rather essential cytological rearrangements and activation of both cellular and humoral immunity. Accordingly to the type of the reaction, two groups of peripheral organs of immunity can be distinguish: the first--the appendix and the iliocecal lymph node which predominantly participate in the immunoblastic reaction and in the reaction of young plasmatic cells and demonstrate increased signs of immature cells emigration; the second--the axillary lymph node, as a representative of somatic nodes, and the spleen--the main carriers of the reaction in mature plasmic cells. Structural peculiarities of the complex appendix--iliocecal lymph node in intact animals and in the experiment make it possible to consider the complex as a peculiar functional link of the immune peripheral organs.", "contents": "[Dynamics of structural changes in the organs of immunity of rabbits with acute experimental appendicitis]. Cyto- and histological analysis of the whole immunogenic system at the development of an acute experimental appendicitis in rabbits has demonstrated that all the constituent parts of the system are involved into the reaction. In the central organ of immunity--thymus the main change is its progressive exhaustion, while peripheral organs of immunity are subjected to rather essential cytological rearrangements and activation of both cellular and humoral immunity. Accordingly to the type of the reaction, two groups of peripheral organs of immunity can be distinguish: the first--the appendix and the iliocecal lymph node which predominantly participate in the immunoblastic reaction and in the reaction of young plasmatic cells and demonstrate increased signs of immature cells emigration; the second--the axillary lymph node, as a representative of somatic nodes, and the spleen--the main carriers of the reaction in mature plasmic cells. Structural peculiarities of the complex appendix--iliocecal lymph node in intact animals and in the experiment make it possible to consider the complex as a peculiar functional link of the immune peripheral organs."}
{"id": "PMID:485867", "title": "[Electron microscopy of human articular cartilage].", "content": "Scanning and transmission microscopy of the articular cartilage was performed in femoral condyles of persons at the age of 30-50 years. It was demonstrated that hyaline cartilage is covered with a protective fibrillar layer consisting of tightly pressed collagenous fibrillae with an underlying layer of fibroblastic cells. In the intracellular substance of the hyaline cartilage fibrillar structures form a complex reticular web with vertical arrangement of the main collagenous fasiculi. In the superficial layer of the hyaline cartilage the collagenous fibrillae and their fasciculi form arcade-like structures. Lacunar chondrocytes have a rough villose surface, cellular secrete is discharged as round granules through cytoplasmic membrane. Ultrastructural changes in chondrocytes are observed simultaneously with their degenerative-dystrophic changes.", "contents": "[Electron microscopy of human articular cartilage]. Scanning and transmission microscopy of the articular cartilage was performed in femoral condyles of persons at the age of 30-50 years. It was demonstrated that hyaline cartilage is covered with a protective fibrillar layer consisting of tightly pressed collagenous fibrillae with an underlying layer of fibroblastic cells. In the intracellular substance of the hyaline cartilage fibrillar structures form a complex reticular web with vertical arrangement of the main collagenous fasiculi. In the superficial layer of the hyaline cartilage the collagenous fibrillae and their fasciculi form arcade-like structures. Lacunar chondrocytes have a rough villose surface, cellular secrete is discharged as round granules through cytoplasmic membrane. Ultrastructural changes in chondrocytes are observed simultaneously with their degenerative-dystrophic changes."}
{"id": "PMID:485868", "title": "[Chondrocyte differentiation and bone growth during the development of the cartilaginous skeleton of chickens].", "content": "Certain local alterations in functional and reproductive activity of chondrocytes were stated at the development of the cartilage skeleton. In epiphyses chondrocytes gradually pass into the phase of rest (G0) with subsequent multiplication during the process of skeletal development. In these structures biosynthesis of nonsulfated proteoglycans predominate, in time, while in other cartilage zones--that of sulfated ones. Proofs are furnished on gradual transition of epiphyseal chondrocytes into the state peculiar for cells of the proliferative zone accompanied by an intensified biosynthesis of sulfated proteoglycans and collagenous proteins. Owing to these peculiarities they can be compared with the cells of the reserve zone in the mammalian metaepiphyseal cartilage. It was stated that intensity of chondrogenesis and growth of bones are affected by several processes: intensity of chondrocyte multiplication, the rate of their repeated division in the proliferative zone, the velosity with which the cells transfer into the state of hypertrophy and the rate of the periostal bone formation at the border-line of metaphysis and diaphysis.", "contents": "[Chondrocyte differentiation and bone growth during the development of the cartilaginous skeleton of chickens]. Certain local alterations in functional and reproductive activity of chondrocytes were stated at the development of the cartilage skeleton. In epiphyses chondrocytes gradually pass into the phase of rest (G0) with subsequent multiplication during the process of skeletal development. In these structures biosynthesis of nonsulfated proteoglycans predominate, in time, while in other cartilage zones--that of sulfated ones. Proofs are furnished on gradual transition of epiphyseal chondrocytes into the state peculiar for cells of the proliferative zone accompanied by an intensified biosynthesis of sulfated proteoglycans and collagenous proteins. Owing to these peculiarities they can be compared with the cells of the reserve zone in the mammalian metaepiphyseal cartilage. It was stated that intensity of chondrogenesis and growth of bones are affected by several processes: intensity of chondrocyte multiplication, the rate of their repeated division in the proliferative zone, the velosity with which the cells transfer into the state of hypertrophy and the rate of the periostal bone formation at the border-line of metaphysis and diaphysis."}
{"id": "PMID:485869", "title": "[Demonstration of brain capillaries using acetyl-CoA].", "content": "The method is based on a reduced procedure for revealing cholinacetyltransferase when the stage of preincubation treatment of the material and inhibitor is omitted. The possibility to investigate the human and animal brains with a complete revealing of the capillary network, which corresponds to injection of the vessels with Indian ink, is proved.", "contents": "[Demonstration of brain capillaries using acetyl-CoA]. The method is based on a reduced procedure for revealing cholinacetyltransferase when the stage of preincubation treatment of the material and inhibitor is omitted. The possibility to investigate the human and animal brains with a complete revealing of the capillary network, which corresponds to injection of the vessels with Indian ink, is proved."}
{"id": "PMID:485871", "title": "[Simple flow chamber for embryos].", "content": "The chamber is made of two subject glasses arranged in such a way that nutritional medium can flow through it. In order to prevent washing out of the embryos by the stream of nutritional medium, a porous packing is inserted through which the nutritional medium is flowing. The upper subject glass has a hole through which the embryos are put into the chamber. The hole is covered with a covering glass. The chamber can be preserved for a long time \"avaiting\" for the cultivated material at any regime of the stream or at a static regime.", "contents": "[Simple flow chamber for embryos]. The chamber is made of two subject glasses arranged in such a way that nutritional medium can flow through it. In order to prevent washing out of the embryos by the stream of nutritional medium, a porous packing is inserted through which the nutritional medium is flowing. The upper subject glass has a hole through which the embryos are put into the chamber. The hole is covered with a covering glass. The chamber can be preserved for a long time \"avaiting\" for the cultivated material at any regime of the stream or at a static regime."}
{"id": "PMID:485872", "title": "[Current clinical medicine and the problem of a \"second\" disease].", "content": "Different interpretations of the term \"second disease\" are discussed. Most attention is given to the \"second disease\" as an independent suffering which in the majority of cases develops in connection with medical interference. The causes, pathogenesis, and practical importance of \"second diseases\" at the present stage of the development of clinical medicine, and their place in the pathoanatomic diagnosis and in tanatogenesis are considered.", "contents": "[Current clinical medicine and the problem of a \"second\" disease]. Different interpretations of the term \"second disease\" are discussed. Most attention is given to the \"second disease\" as an independent suffering which in the majority of cases develops in connection with medical interference. The causes, pathogenesis, and practical importance of \"second diseases\" at the present stage of the development of clinical medicine, and their place in the pathoanatomic diagnosis and in tanatogenesis are considered."}
{"id": "PMID:485873", "title": "[Degree of maturity and histogenesis of stomach cancer and their importance for prognosis].", "content": "The intensity of differentiation of adenocarcinoma and non-differentiated cancer cells was studied histochemically and electron microscopically. A higher ratio of nucleus volume to cytoplasm volume and nucleus nuclei acids to cytoplasm RNA, low secretory activity in adenocarcinoma cells as compared to nondifferentiated cancers were found. Adenocarcinomas were shown to have a higher activity of NADP-dependent dehydrogenases, more numerous free ribosomes as compared to nondifferentiated carcinoma. This suggests a lower degree of cell differentiation and high proliferative activity in adenocarcinomas than in nondifferentiated carcinoma. Differences in the intensity of cell and tissue differentiation in adenocarcinomas and nondifferentiated carcinoma and due to differences in their histogenesis and must be taken into account in evaluations of the prognosis.", "contents": "[Degree of maturity and histogenesis of stomach cancer and their importance for prognosis]. The intensity of differentiation of adenocarcinoma and non-differentiated cancer cells was studied histochemically and electron microscopically. A higher ratio of nucleus volume to cytoplasm volume and nucleus nuclei acids to cytoplasm RNA, low secretory activity in adenocarcinoma cells as compared to nondifferentiated cancers were found. Adenocarcinomas were shown to have a higher activity of NADP-dependent dehydrogenases, more numerous free ribosomes as compared to nondifferentiated carcinoma. This suggests a lower degree of cell differentiation and high proliferative activity in adenocarcinomas than in nondifferentiated carcinoma. Differences in the intensity of cell and tissue differentiation in adenocarcinomas and nondifferentiated carcinoma and due to differences in their histogenesis and must be taken into account in evaluations of the prognosis."}
{"id": "PMID:485874", "title": "[Complication of resuscitation and intensive therapy].", "content": "On the basis of data from the literature and the author's own observations the paper analyses complications of resuscitation and intensive therapy recently classified as a special part of human pathology (resuscitation pathology). A classification of this pathology is suggested, indicating most important complications, causes of their development and preventive measures. Examples of changes in the clinico-morphological picture of the main disease under the influence of resuscitation and intensive therapy are presented.", "contents": "[Complication of resuscitation and intensive therapy]. On the basis of data from the literature and the author's own observations the paper analyses complications of resuscitation and intensive therapy recently classified as a special part of human pathology (resuscitation pathology). A classification of this pathology is suggested, indicating most important complications, causes of their development and preventive measures. Examples of changes in the clinico-morphological picture of the main disease under the influence of resuscitation and intensive therapy are presented."}
{"id": "PMID:485875", "title": "[Morphological characteristics of early stomach cancer (analysis of 150 cases)].", "content": "One hundred and fifty early carcinomas of the stomach were studied. Morphology of 5 anatomic types (polypoid, raised, flat, superficial ulceration, ulcerative) corresponding to endoscopic types of early carcinoma is described. Previous diseases, age and sex of patients were found to influence the macroscopic and histological structure of the tumour, the rate of invasion of the submucosa. Therefore, the authors distinguish a) early carcinoma in the presence of polyps or chronic gastritis and b) carcinoma in the presence of peptic ulcer. The latter comprises nearly 80% of all early carcinomas. Three zones where the tumor arises were found: angle of the stomach, pyloric fold, and the border between the esophagus and stomach. Various forms of carcinoma have different rates of affection of each of these zones.", "contents": "[Morphological characteristics of early stomach cancer (analysis of 150 cases)]. One hundred and fifty early carcinomas of the stomach were studied. Morphology of 5 anatomic types (polypoid, raised, flat, superficial ulceration, ulcerative) corresponding to endoscopic types of early carcinoma is described. Previous diseases, age and sex of patients were found to influence the macroscopic and histological structure of the tumour, the rate of invasion of the submucosa. Therefore, the authors distinguish a) early carcinoma in the presence of polyps or chronic gastritis and b) carcinoma in the presence of peptic ulcer. The latter comprises nearly 80% of all early carcinomas. Three zones where the tumor arises were found: angle of the stomach, pyloric fold, and the border between the esophagus and stomach. Various forms of carcinoma have different rates of affection of each of these zones."}
{"id": "PMID:485876", "title": "[Intestinal metaplasia in glandular and cystic formations and human urothelial adenocarcinoma].", "content": "Thirteen cases of inflammatory changes in the urothelium with formation of glandular and cystous structures, 3 cases of glandular metaplasia in transitional cell carcinoma and 8 adenocarcinomas were examined. In inflammation, initial glandular and cystic structures have signs of somehow incompleted metaplasia of the intestinal type. In advanced glandular structures metaplasia is completed and urothelium acquires similarity in the structure and properties of the produced mucus with the large intestine epithelium (numerous goblet, cylindrical limbic and non-differentiated cells, prevalence of argentaffinic cells over argirophilic, lack of Panet cells, secretion of sulfomucines). In loci of glandular metaplasia of transitional cell carcinoma there are also signs of somehow incompleted intestinal differentiation. Most typical variants of urothelial adenocarcinoma are quite similar to glandular carcinoma by the structure and properties of the produced mucus.", "contents": "[Intestinal metaplasia in glandular and cystic formations and human urothelial adenocarcinoma]. Thirteen cases of inflammatory changes in the urothelium with formation of glandular and cystous structures, 3 cases of glandular metaplasia in transitional cell carcinoma and 8 adenocarcinomas were examined. In inflammation, initial glandular and cystic structures have signs of somehow incompleted metaplasia of the intestinal type. In advanced glandular structures metaplasia is completed and urothelium acquires similarity in the structure and properties of the produced mucus with the large intestine epithelium (numerous goblet, cylindrical limbic and non-differentiated cells, prevalence of argentaffinic cells over argirophilic, lack of Panet cells, secretion of sulfomucines). In loci of glandular metaplasia of transitional cell carcinoma there are also signs of somehow incompleted intestinal differentiation. Most typical variants of urothelial adenocarcinoma are quite similar to glandular carcinoma by the structure and properties of the produced mucus."}
{"id": "PMID:485877", "title": "[Pathogenetic mechanisms of cyst formation in the breasts and mammae].", "content": "Mammary glands and endocrine organs of human females ranging in ages from 6 months to 25 years (228 cases) as well as in intact, castrated, hypophysectomized, thyroidectomized, adrenalectomized young virgin female rats treated with steroid hormones and thyroxin were studied by various morphological methods. The process of cyst formation in female mammary glands was found to be enhanced by intensified endocrine influences on them in the interval of 6 months to 2 1/2 years (the effect of maternal hormones received by the baby with milk) and from 11 to 15 years (physiological activation of endocrine glands) and to decline in the period of relative quiescence at the age of 2 1/2 to 11 years. Both hyperestrogenism and hypercorticism and hypofunction of the thyroid gland as well as dysfunction of adenohypophysis and neuro-trophic apparatus of mammary glands are important in the pathogenesis of cysts.", "contents": "[Pathogenetic mechanisms of cyst formation in the breasts and mammae]. Mammary glands and endocrine organs of human females ranging in ages from 6 months to 25 years (228 cases) as well as in intact, castrated, hypophysectomized, thyroidectomized, adrenalectomized young virgin female rats treated with steroid hormones and thyroxin were studied by various morphological methods. The process of cyst formation in female mammary glands was found to be enhanced by intensified endocrine influences on them in the interval of 6 months to 2 1/2 years (the effect of maternal hormones received by the baby with milk) and from 11 to 15 years (physiological activation of endocrine glands) and to decline in the period of relative quiescence at the age of 2 1/2 to 11 years. Both hyperestrogenism and hypercorticism and hypofunction of the thyroid gland as well as dysfunction of adenohypophysis and neuro-trophic apparatus of mammary glands are important in the pathogenesis of cysts."}
{"id": "PMID:485878", "title": "[Myelinolysis of the pons Varolli].", "content": "A case of myelinolysis of pons varolli which was diagnosed only after a microscopic examination of the brain is described. Certain morphological features of the focus in pons varolli were observed. It is assumed that in this observation the development of central pontine myelinosis was influenced by the abuse of alcohol as well as by exacerbation of pulmonary tuberculosis.", "contents": "[Myelinolysis of the pons Varolli]. A case of myelinolysis of pons varolli which was diagnosed only after a microscopic examination of the brain is described. Certain morphological features of the focus in pons varolli were observed. It is assumed that in this observation the development of central pontine myelinosis was influenced by the abuse of alcohol as well as by exacerbation of pulmonary tuberculosis."}
{"id": "PMID:485879", "title": "[Case of trichinosis with lethal outcome due to consumption of brown bear meat in the Krasnoyarsk Territory].", "content": "One autopsy case of trichinellosis (in a man of 22) with extensive involvement of the locomotor apparatus (secondary infectious-allergic polymyositis) is presented. Group infection with trichinellosis occurred by eating of insufficiently treated meat of a brown bear which confirms the existence of a natural focus of trichinellosis in the Krasnoyarsky kray.", "contents": "[Case of trichinosis with lethal outcome due to consumption of brown bear meat in the Krasnoyarsk Territory]. One autopsy case of trichinellosis (in a man of 22) with extensive involvement of the locomotor apparatus (secondary infectious-allergic polymyositis) is presented. Group infection with trichinellosis occurred by eating of insufficiently treated meat of a brown bear which confirms the existence of a natural focus of trichinellosis in the Krasnoyarsky kray."}
{"id": "PMID:485882", "title": "[Regenerative reactions of myocardiocyte nuclei in ischemic heart disease].", "content": "The ploidy of myocardiocytes nuclei in the left ventricle was determined stereometrically and microspectrophotometrically in 20 cases of ischemic heart disease (IHD) and 9 control cases. An increase in the number of nuclei and the degree of their ploidy and their correlation with the myocardium mass were established. The number of polyploid nuclei was particularly high in the periphery of postinfarction scars. These data indirectly indicate the participation of polyploidization and amitosis of myocardiocytes nuclei in the development of myocardial regenerative hypertrophy.", "contents": "[Regenerative reactions of myocardiocyte nuclei in ischemic heart disease]. The ploidy of myocardiocytes nuclei in the left ventricle was determined stereometrically and microspectrophotometrically in 20 cases of ischemic heart disease (IHD) and 9 control cases. An increase in the number of nuclei and the degree of their ploidy and their correlation with the myocardium mass were established. The number of polyploid nuclei was particularly high in the periphery of postinfarction scars. These data indirectly indicate the participation of polyploidization and amitosis of myocardiocytes nuclei in the development of myocardial regenerative hypertrophy."}
{"id": "PMID:485883", "title": "[Intraoperative damage to the myocardium and heart failure upon radical correction of the tetralogy of Fallot].", "content": "The ultrastructure of contractile myocardium at the stages of anoxia and after switching in the coronary circulation was studied on the material of 128 biopsies of the myocardium from 51 patients with Fallot's tetrad operated radically under conditions of artificial circulation and anoxic cardiac arrest. Heterogeneity of cell organelles damage made it difficult to evaluate myocardial injury on the whole. To make morphological data more objective, a geometric method of quantitative analysis was used which permitted to distinguish two groups of damage and to correlate them with the pattern of cardiac activity restoration and the course of the postoperational period. The results indicate some relationship between changes in the ultrastructure of mitochondria and the development of cardiac insufficiency in patients with Fallot's tetrad.", "contents": "[Intraoperative damage to the myocardium and heart failure upon radical correction of the tetralogy of Fallot]. The ultrastructure of contractile myocardium at the stages of anoxia and after switching in the coronary circulation was studied on the material of 128 biopsies of the myocardium from 51 patients with Fallot's tetrad operated radically under conditions of artificial circulation and anoxic cardiac arrest. Heterogeneity of cell organelles damage made it difficult to evaluate myocardial injury on the whole. To make morphological data more objective, a geometric method of quantitative analysis was used which permitted to distinguish two groups of damage and to correlate them with the pattern of cardiac activity restoration and the course of the postoperational period. The results indicate some relationship between changes in the ultrastructure of mitochondria and the development of cardiac insufficiency in patients with Fallot's tetrad."}
{"id": "PMID:485884", "title": "[Morphogenesis of acute pyelonephritis (electron microscopic study)].", "content": "Investigations of fine changes of nephron elements in acute pyelonephritis against the background of urolithiasis in 19 patients and 43 rabbits showed the developing morphological lesions in the kidneys to be due to combined effect of the inflammatory and ischemic factors. The inflammation began with the development of serous oedema leading to destruction of tubular epithelium and impairment of urine passage. These lesions contributed to transformation of the serous inflammation into purulent one with formation of histolysis foci. The ischemic factor progressed with the advance of the inflammatory process and was aggravated by the development of foci of histolysis and hephrohydrosis and subsequently of vascular-parenchymatous block at the tubular level.", "contents": "[Morphogenesis of acute pyelonephritis (electron microscopic study)]. Investigations of fine changes of nephron elements in acute pyelonephritis against the background of urolithiasis in 19 patients and 43 rabbits showed the developing morphological lesions in the kidneys to be due to combined effect of the inflammatory and ischemic factors. The inflammation began with the development of serous oedema leading to destruction of tubular epithelium and impairment of urine passage. These lesions contributed to transformation of the serous inflammation into purulent one with formation of histolysis foci. The ischemic factor progressed with the advance of the inflammatory process and was aggravated by the development of foci of histolysis and hephrohydrosis and subsequently of vascular-parenchymatous block at the tubular level."}
{"id": "PMID:485885", "title": "[Histochemical study of fat in erythema nodosum].", "content": "Histochemical study of fat in the adipocytes of the subcutaneous adipose tissue in 12 patients with erythema nodosum revealed an upset balance of the oxidation process in fat and accumulation of free peroxide and aldehyde groups. The imbalance in the oxidation processes in the adipocytes in the areas of hemorrhages is apparently associated with haemin catalysis. The accumulation of incomplete oxidation products in lesions is believed to be a local process caused by pathological changes in the blood vessels. The toxicity of the products of peroxide oxidation should be taken into consideration in treating patients with erythema nodosum.", "contents": "[Histochemical study of fat in erythema nodosum]. Histochemical study of fat in the adipocytes of the subcutaneous adipose tissue in 12 patients with erythema nodosum revealed an upset balance of the oxidation process in fat and accumulation of free peroxide and aldehyde groups. The imbalance in the oxidation processes in the adipocytes in the areas of hemorrhages is apparently associated with haemin catalysis. The accumulation of incomplete oxidation products in lesions is believed to be a local process caused by pathological changes in the blood vessels. The toxicity of the products of peroxide oxidation should be taken into consideration in treating patients with erythema nodosum."}
{"id": "PMID:485886", "title": "[Mitotic conditions in primary and metastatic nodes of breast and cervix cancer].", "content": "The results of the study on the mitotic regimen (mitotic activity ratios of mitosis phases and percent of pathological mitoses) in primary and metastatic nodes of the tumour by the example of mammary gland and cervical carcinoma are presented. A similarity in the mitotic regimen in primary and metastatic nodes of the tumour was observed which was manifested in a high level of mitotic activity predominance of metaphases over other phases of mitosis, the occurrence of similar pathological forms of mitosis. The difference between the mitotic regimen in the primary and metastatic nodes consisted in an increase in the latter of the number of pathological mitoses mostly at the expence of K-mitoses. Three-group metaphase typical of cervical carcinoma was found in all metastatic nodes of the squamous cell carcinoma of the cervix and may probably serve as a peculiar \"marker\" of tumours of this localization.", "contents": "[Mitotic conditions in primary and metastatic nodes of breast and cervix cancer]. The results of the study on the mitotic regimen (mitotic activity ratios of mitosis phases and percent of pathological mitoses) in primary and metastatic nodes of the tumour by the example of mammary gland and cervical carcinoma are presented. A similarity in the mitotic regimen in primary and metastatic nodes of the tumour was observed which was manifested in a high level of mitotic activity predominance of metaphases over other phases of mitosis, the occurrence of similar pathological forms of mitosis. The difference between the mitotic regimen in the primary and metastatic nodes consisted in an increase in the latter of the number of pathological mitoses mostly at the expence of K-mitoses. Three-group metaphase typical of cervical carcinoma was found in all metastatic nodes of the squamous cell carcinoma of the cervix and may probably serve as a peculiar \"marker\" of tumours of this localization."}
{"id": "PMID:485887", "title": "[Solitary plasmacytoma of the hypothalamus].", "content": "A rare case of intracranial extra osseal isolated plasmocytoma in a girl of 18 is presented. The tumour localized in the hypothalamus and extended into the intracranial parts of the optic nerves and chiasma opticum. It consisted of mature and immature plasma cells and contained paraamyloid depositions. The disease had a progredient course accompanied by the disorders of vision, endocrine-metabolic disturbances and hyperglobulinemia.", "contents": "[Solitary plasmacytoma of the hypothalamus]. A rare case of intracranial extra osseal isolated plasmocytoma in a girl of 18 is presented. The tumour localized in the hypothalamus and extended into the intracranial parts of the optic nerves and chiasma opticum. It consisted of mature and immature plasma cells and contained paraamyloid depositions. The disease had a progredient course accompanied by the disorders of vision, endocrine-metabolic disturbances and hyperglobulinemia."}
{"id": "PMID:485888", "title": "The variable natural history of subacute sclerosing panencephalitis: a study of 118 cases from the Middle East.", "content": "A variable natural history was found in a standardized follow-up study of 118 patients with subacute sclerosing panencephalitis (SSPE). Only 20% followed the sequence:behavior change, mental deterioration, periodic attacks, severe debility, and death within a year. Six stages were identified: 0, subtle psychointellectual symptoms; 1, obvious psychointellectual and neurological changes; 2, stereotyped attacks; 3, vegetative psychomotor condition; 4, improvement; 5, relapse. Illness began in stage 0 for 32%; 1, 36%; 2, 19%; 1 and 2 simultaneously, 13%. In early development, 77% were hyperactive, and 27% had psychointellectual difficulties one to five years prior to obvious illness. The average duration of each stage was as follows: stage 0, two years; 1, 2.5 months; 2, 7.5 months; 3, 5.5 months; 4, 3.5 years; 5, 1.5 years. Noteworthy improvements and plateaus occurred in more than half of the patients. In stage 4, improvement was long-term and substantial for 5% and modest for 18%. Survival followed an exponential curve with an average half-life of 1.8 years; 41% of the patients survived beyond two years. Half of the patients passed through neither stage 0 nor stage 4; their half-life was 0.7 years. The remainder had a half-life of 3.0 years.", "contents": "The variable natural history of subacute sclerosing panencephalitis: a study of 118 cases from the Middle East. A variable natural history was found in a standardized follow-up study of 118 patients with subacute sclerosing panencephalitis (SSPE). Only 20% followed the sequence:behavior change, mental deterioration, periodic attacks, severe debility, and death within a year. Six stages were identified: 0, subtle psychointellectual symptoms; 1, obvious psychointellectual and neurological changes; 2, stereotyped attacks; 3, vegetative psychomotor condition; 4, improvement; 5, relapse. Illness began in stage 0 for 32%; 1, 36%; 2, 19%; 1 and 2 simultaneously, 13%. In early development, 77% were hyperactive, and 27% had psychointellectual difficulties one to five years prior to obvious illness. The average duration of each stage was as follows: stage 0, two years; 1, 2.5 months; 2, 7.5 months; 3, 5.5 months; 4, 3.5 years; 5, 1.5 years. Noteworthy improvements and plateaus occurred in more than half of the patients. In stage 4, improvement was long-term and substantial for 5% and modest for 18%. Survival followed an exponential curve with an average half-life of 1.8 years; 41% of the patients survived beyond two years. Half of the patients passed through neither stage 0 nor stage 4; their half-life was 0.7 years. The remainder had a half-life of 3.0 years."}
{"id": "PMID:485889", "title": "Amyotrophic lateral sclerosis with ophthalmoplegia. A clinicopathologic study.", "content": "Ophthalmoplegia is rarely observed in patients with amyotrophic lateral sclerosis (ALS). We describe a patient with ALS in whom ophthalmoplegia, initially appearing midway in her course, progressed to total paralysis of extraocular movements by the time of death. In addition to the usual postmortem findings of ALS in the brain stem and spinal cord, there was extensive neuronal loss and gliosis involving the caudal portions of the dorsal and intermediate components of the oculomotor nuclei, the caudal part of the trochlear nuclei, and the abducens nuclei. This represents the first detailed report of the findings in the nuclei of cranial nerves III, IV, and VI in a patient with ALS and ophthalmoplegia.", "contents": "Amyotrophic lateral sclerosis with ophthalmoplegia. A clinicopathologic study. Ophthalmoplegia is rarely observed in patients with amyotrophic lateral sclerosis (ALS). We describe a patient with ALS in whom ophthalmoplegia, initially appearing midway in her course, progressed to total paralysis of extraocular movements by the time of death. In addition to the usual postmortem findings of ALS in the brain stem and spinal cord, there was extensive neuronal loss and gliosis involving the caudal portions of the dorsal and intermediate components of the oculomotor nuclei, the caudal part of the trochlear nuclei, and the abducens nuclei. This represents the first detailed report of the findings in the nuclei of cranial nerves III, IV, and VI in a patient with ALS and ophthalmoplegia."}
{"id": "PMID:485890", "title": "Upward transtentorial herniation: seven cases and a literature review.", "content": "Seven cases of upward transtentorial herniation occurred. In each patient, coma with reactive, miotic pupils, asymmetrical or absent caloric responses, and decerebrate posture indicated brain-stem compression. In this setting, the development of unequal, then midposition, fixed pupils signaled midbrain failure from upward herniation. Vertebral angiography showed upward displacement of the superior cerebellar arteries. Results of autopsy confirmed the existence of grooving of the vermis by the tentorial margins and, in one case, of anterior displacement and distortion of the midbrain. In five of 45 reported cases of upward herniation, the conditions were diagnosed antemortem. Instances of cerebellar hematoma and tumor predominated. In at least seven patients, performance of ventriculography may have precipitated herniation. Clinical details were provided in only nine patients and did not separate upward herniation from brain-stem compression. Cerebellar ischemic infarct found in one of our patients is a rarely reported cause of upward herniation.", "contents": "Upward transtentorial herniation: seven cases and a literature review. Seven cases of upward transtentorial herniation occurred. In each patient, coma with reactive, miotic pupils, asymmetrical or absent caloric responses, and decerebrate posture indicated brain-stem compression. In this setting, the development of unequal, then midposition, fixed pupils signaled midbrain failure from upward herniation. Vertebral angiography showed upward displacement of the superior cerebellar arteries. Results of autopsy confirmed the existence of grooving of the vermis by the tentorial margins and, in one case, of anterior displacement and distortion of the midbrain. In five of 45 reported cases of upward herniation, the conditions were diagnosed antemortem. Instances of cerebellar hematoma and tumor predominated. In at least seven patients, performance of ventriculography may have precipitated herniation. Clinical details were provided in only nine patients and did not separate upward herniation from brain-stem compression. Cerebellar ischemic infarct found in one of our patients is a rarely reported cause of upward herniation."}
{"id": "PMID:485891", "title": "Administration of human somatotropin in levodopa-treated patients with Parkinsonism.", "content": "To investigate further the role of growth hormone (GH) on the cerebral effects of levodopa, human somatotropin (human growth hormone) was administered to four patients with parkinsonism with varying response to chronic levodopa therapy. The doses of somatotropin (5 IU) were administered on alternate days for four days, and the effects of this hormone on the patient's symptomatic control, dyskinesia, plasma GH, and dopa levels were evaluated and compared with those of patients receiving saline injections. We have demonstrated that administration of exogenous human somatotropin, even during marked and sustained elevations of plasma GH levels, does not alter the therapeutic or side effects of levodopa therapy. With one exception, plasma dopa levels after human somatotropin administration remained unchanged. We conclude that neither the endogenous increases of GH in response to levodopa nor those attained following human somatotropin administration modify in any consistent way the therapeutic and side effects of chronic levodopa therapy, and that the episodic releases of GH in response to levodopa occur independently of its cerebral effects.", "contents": "Administration of human somatotropin in levodopa-treated patients with Parkinsonism. To investigate further the role of growth hormone (GH) on the cerebral effects of levodopa, human somatotropin (human growth hormone) was administered to four patients with parkinsonism with varying response to chronic levodopa therapy. The doses of somatotropin (5 IU) were administered on alternate days for four days, and the effects of this hormone on the patient's symptomatic control, dyskinesia, plasma GH, and dopa levels were evaluated and compared with those of patients receiving saline injections. We have demonstrated that administration of exogenous human somatotropin, even during marked and sustained elevations of plasma GH levels, does not alter the therapeutic or side effects of levodopa therapy. With one exception, plasma dopa levels after human somatotropin administration remained unchanged. We conclude that neither the endogenous increases of GH in response to levodopa nor those attained following human somatotropin administration modify in any consistent way the therapeutic and side effects of chronic levodopa therapy, and that the episodic releases of GH in response to levodopa occur independently of its cerebral effects."}
{"id": "PMID:485892", "title": "Hypercapnic alteration of visual evoked responses in acute cerebral infarction.", "content": "To determine the effect of inhaled carbon dioxide on acute ischemic cerebral injury, we have compared occipital visual evoked responses (VER) at baseline and during hypercapnia in 20 patients with acute unilateral cerebral infarction (ten with and ten without homonymous hemianopsia) and in ten normal controls. Visual evoked responses were judged on the basis of interhemispheral symmetry. In eight of ten controls and six of 20 patients, baseline VERs were symmetrical and remained unchanged during hypercapnia. In 14 patients with asymmetrical baseline VERs, hypercapnia caused improvement of symmetry in five, worsening in three, and no change in six. Hypercapnic vasodilation may be either beneficial or deleterious to cerebral function in patients with acute cerebral infarction.", "contents": "Hypercapnic alteration of visual evoked responses in acute cerebral infarction. To determine the effect of inhaled carbon dioxide on acute ischemic cerebral injury, we have compared occipital visual evoked responses (VER) at baseline and during hypercapnia in 20 patients with acute unilateral cerebral infarction (ten with and ten without homonymous hemianopsia) and in ten normal controls. Visual evoked responses were judged on the basis of interhemispheral symmetry. In eight of ten controls and six of 20 patients, baseline VERs were symmetrical and remained unchanged during hypercapnia. In 14 patients with asymmetrical baseline VERs, hypercapnia caused improvement of symmetry in five, worsening in three, and no change in six. Hypercapnic vasodilation may be either beneficial or deleterious to cerebral function in patients with acute cerebral infarction."}
{"id": "PMID:485893", "title": "Computerized tomography in intracranial metastases.", "content": "Two-hundred consecutive patients with suspected metastatic carcinoma and signs of cerebral dysfunction were evaluated by computerized tomography (CT). In ten patients, CT defined a nonneoplastic disorder, whereas in 190, CT defined a metastatic neoplasm. In 70 of these patients, the lesion was solitary; in 120, the lesions were multiple. In 52 patients, plain scan showed a solitary lesion, but multiple lesions were seen after contrast scan. In nine patients, the abnormality was seen only on postcontrast scan. One hundred lesions were of low density and 87 showed enhancement; 81 were of high density, usually surrounded by a low-density rim, and 78 were enhanced. There were no characteristic CT patterns of specific systemic carcinomas, but epidermoid carcinoma frequently appeared as a low-density lesion with a thin peripheral enhancing rim, and adenocarcinoma appeared as a dense, homogeneous, round, enhancing nodule. After treatment, enhancement was the most reliable sign of residual or recurrent tumor.", "contents": "Computerized tomography in intracranial metastases. Two-hundred consecutive patients with suspected metastatic carcinoma and signs of cerebral dysfunction were evaluated by computerized tomography (CT). In ten patients, CT defined a nonneoplastic disorder, whereas in 190, CT defined a metastatic neoplasm. In 70 of these patients, the lesion was solitary; in 120, the lesions were multiple. In 52 patients, plain scan showed a solitary lesion, but multiple lesions were seen after contrast scan. In nine patients, the abnormality was seen only on postcontrast scan. One hundred lesions were of low density and 87 showed enhancement; 81 were of high density, usually surrounded by a low-density rim, and 78 were enhanced. There were no characteristic CT patterns of specific systemic carcinomas, but epidermoid carcinoma frequently appeared as a low-density lesion with a thin peripheral enhancing rim, and adenocarcinoma appeared as a dense, homogeneous, round, enhancing nodule. After treatment, enhancement was the most reliable sign of residual or recurrent tumor."}
{"id": "PMID:485894", "title": "Subacute necrotizing polioencephalopathy.", "content": "A previously healthy 20-year-old woman died of a progressive cerebral disease that involved the brain stem and the cerebral hemispheres successively. The illness ran its course in 80 days. Postmortem examination showed a multifocal necrotizing encephalopathy that chiefly affected gray matter structures and that was mainly located in the brain stem and cerebral cortex. The cause and pathogenesis remain unknown.", "contents": "Subacute necrotizing polioencephalopathy. A previously healthy 20-year-old woman died of a progressive cerebral disease that involved the brain stem and the cerebral hemispheres successively. The illness ran its course in 80 days. Postmortem examination showed a multifocal necrotizing encephalopathy that chiefly affected gray matter structures and that was mainly located in the brain stem and cerebral cortex. The cause and pathogenesis remain unknown."}
{"id": "PMID:485895", "title": "Devic's syndrome and systemic lupus erythematosus: a case report with necropsy.", "content": "Systemic lupus erythematosus (SLE) rarely occurs coincident with demyelinating disease and, to our knowledge, only one previous case of Devic's syndrome with SLE has been reported.", "contents": "Devic's syndrome and systemic lupus erythematosus: a case report with necropsy. Systemic lupus erythematosus (SLE) rarely occurs coincident with demyelinating disease and, to our knowledge, only one previous case of Devic's syndrome with SLE has been reported."}
{"id": "PMID:485896", "title": "Neuropathy with susceptibility to compression aggravated by herniated disk. Early pathological and electrodiagnostic studies.", "content": "We describe a patient with a foot drop attributable to both herniated disk and peripheral neuropathy susceptible to external compression. Early electrodiagnostic studies and sural nerve biopsy indicated a role for myelin dysfunction in this disorder.", "contents": "Neuropathy with susceptibility to compression aggravated by herniated disk. Early pathological and electrodiagnostic studies. We describe a patient with a foot drop attributable to both herniated disk and peripheral neuropathy susceptible to external compression. Early electrodiagnostic studies and sural nerve biopsy indicated a role for myelin dysfunction in this disorder."}
{"id": "PMID:485897", "title": "Successful treatment of narcolepsy with propranolol: a case report.", "content": "A patient with severe narcolepsy and cataplexy had been treated with a high dosage of methylphenidate hydrochloride, but the drug was not effective. To relieve the patient's cardiac arrhythmia, which was assumed to be secondary to drug therapy, we withdrew methylphenidate therapy and started propranolol hydrochloride therapy. When the dosage of propranolol was increased to a level consistent with maximum beta-adrenergic receptor blockade, the attacks were eliminated.", "contents": "Successful treatment of narcolepsy with propranolol: a case report. A patient with severe narcolepsy and cataplexy had been treated with a high dosage of methylphenidate hydrochloride, but the drug was not effective. To relieve the patient's cardiac arrhythmia, which was assumed to be secondary to drug therapy, we withdrew methylphenidate therapy and started propranolol hydrochloride therapy. When the dosage of propranolol was increased to a level consistent with maximum beta-adrenergic receptor blockade, the attacks were eliminated."}
{"id": "PMID:485898", "title": "Subarachnoid dissemination of thoracic chordoma.", "content": "Chordoma at the T-9 level, originally manifested by lumbosacral radiculopathy, developed in a 50-year-old man. The patient underwent three operations and radiation therapy for this condition, but ten years later, thoracic myelopathy developed, followed by left facial weakness. On autopsy, extensive dissemination of chordoma was found along the base of the brain and in the leptomeninges of the spinal cord.", "contents": "Subarachnoid dissemination of thoracic chordoma. Chordoma at the T-9 level, originally manifested by lumbosacral radiculopathy, developed in a 50-year-old man. The patient underwent three operations and radiation therapy for this condition, but ten years later, thoracic myelopathy developed, followed by left facial weakness. On autopsy, extensive dissemination of chordoma was found along the base of the brain and in the leptomeninges of the spinal cord."}
{"id": "PMID:485899", "title": "An atypical case of delayed radiation necrosis of the brain.", "content": "A patient had radiation necrosis of the brain after treatment for a chromophobe adenoma of the pituitary. Atypically, and in contrast to most previously reported cases, clinical symptoms and signs were few. The computerized tomographic findings are discussed with a review of the relevant literature.", "contents": "An atypical case of delayed radiation necrosis of the brain. A patient had radiation necrosis of the brain after treatment for a chromophobe adenoma of the pituitary. Atypically, and in contrast to most previously reported cases, clinical symptoms and signs were few. The computerized tomographic findings are discussed with a review of the relevant literature."}
{"id": "PMID:485900", "title": "Benign recurrent aseptic meningitis (Mollaret's meningitis): case report and clinical review.", "content": "Benign recurrent aseptic meningitis (Mollaret's meningitis) is rare. Only five cases have been reported in this country. This article reports another case and briefly reviews the characteristic manifestations of this disorder.", "contents": "Benign recurrent aseptic meningitis (Mollaret's meningitis): case report and clinical review. Benign recurrent aseptic meningitis (Mollaret's meningitis) is rare. Only five cases have been reported in this country. This article reports another case and briefly reviews the characteristic manifestations of this disorder."}
{"id": "PMID:485901", "title": "Visualization of basilar invagination by computerized tomography.", "content": "Computerized tomography was used to visualize bony masses in the posterior fossa in a patient with basilar invagination associated with symptoms of vertebrobasilar insufficiency.", "contents": "Visualization of basilar invagination by computerized tomography. Computerized tomography was used to visualize bony masses in the posterior fossa in a patient with basilar invagination associated with symptoms of vertebrobasilar insufficiency."}
{"id": "PMID:485906", "title": "Provocation and mechanism of angle-closure glaucoma after iridectomy.", "content": "Dark room tests and prone-position tests in a lighted room and in a dark room were carried out on 64 eyes in 32 normal patients and 37 eyes of 30 patients that had undergone complicated iridectomy. Among the iridectomized eyes, the dark room tests and the prone-position test in lighted room caused elevation of intraocular pressure of more than 8 mm Hg in only 2.7% and 16.2%, respectively. Nearly 60% of the eyes showed positive results when the prone-position test was performed in a dark room. Our findings may provide experimental support for the theory of direct lens block as an angle-closure mechanism.", "contents": "Provocation and mechanism of angle-closure glaucoma after iridectomy. Dark room tests and prone-position tests in a lighted room and in a dark room were carried out on 64 eyes in 32 normal patients and 37 eyes of 30 patients that had undergone complicated iridectomy. Among the iridectomized eyes, the dark room tests and the prone-position test in lighted room caused elevation of intraocular pressure of more than 8 mm Hg in only 2.7% and 16.2%, respectively. Nearly 60% of the eyes showed positive results when the prone-position test was performed in a dark room. Our findings may provide experimental support for the theory of direct lens block as an angle-closure mechanism."}
{"id": "PMID:485907", "title": "The human corneal endothelium in keratoconus: A specular microscopic study.", "content": "The corneal endothelium in 12 cases of keratoconus was examined with the clinical specular microscope. There appeared to be an increase in cellular pleomorphism with many cells considerably smaller than normal distributed throughout the endothelial cell population. There were also many large, elongated cells whose long axis showed a definite tendency to assume a similar directional orientation. The long axis of these cells seemed oriented toward the apex of the cone, and the cells appeared to have been stretched by the ectatic process. Many endothelial cells contained dark intracellular structures. Their significance is unknown. The single cornea in this series with a history of acute hydrops contained a localized area in which the endothelial cells were seven to ten times larger than normal. This suggests that rupture of the endothelium and Descemet's membrane, responsible for the acute edematous process, occurs at this site, and that the adjacent cells enlarged to fill the defect.", "contents": "The human corneal endothelium in keratoconus: A specular microscopic study. The corneal endothelium in 12 cases of keratoconus was examined with the clinical specular microscope. There appeared to be an increase in cellular pleomorphism with many cells considerably smaller than normal distributed throughout the endothelial cell population. There were also many large, elongated cells whose long axis showed a definite tendency to assume a similar directional orientation. The long axis of these cells seemed oriented toward the apex of the cone, and the cells appeared to have been stretched by the ectatic process. Many endothelial cells contained dark intracellular structures. Their significance is unknown. The single cornea in this series with a history of acute hydrops contained a localized area in which the endothelial cells were seven to ten times larger than normal. This suggests that rupture of the endothelium and Descemet's membrane, responsible for the acute edematous process, occurs at this site, and that the adjacent cells enlarged to fill the defect."}
{"id": "PMID:485908", "title": "Epithelialization of the anterior chamber: clinical investigation with the specular microscope.", "content": "Four patients, each of whom had had an uncomplicated cataract extraction, were examined because of an apparent epithelialization of the anterior chamber. In each instance, the diagnosis was later verified histopathologically. The involved eye was photographed with the clinical specular microscope and the endothelial photomicrographs were analyzed. It was noted that considerable endothelial cell loss had occurred, as evidence by the larger size of the remaining cells. Endothelial cells were present but they were grossly abnormal well below the demarcation line visible with the slit-lamp biomicroscope. These in vivo observations support the thesis that damage to the corneal endothelium is a necessary factor for epithelial invasion of the anterior chamber.", "contents": "Epithelialization of the anterior chamber: clinical investigation with the specular microscope. Four patients, each of whom had had an uncomplicated cataract extraction, were examined because of an apparent epithelialization of the anterior chamber. In each instance, the diagnosis was later verified histopathologically. The involved eye was photographed with the clinical specular microscope and the endothelial photomicrographs were analyzed. It was noted that considerable endothelial cell loss had occurred, as evidence by the larger size of the remaining cells. Endothelial cells were present but they were grossly abnormal well below the demarcation line visible with the slit-lamp biomicroscope. These in vivo observations support the thesis that damage to the corneal endothelium is a necessary factor for epithelial invasion of the anterior chamber."}
{"id": "PMID:485909", "title": "The value of liver scintigraphy in choroidal melanoma.", "content": "Radionuclide liver scintigraphy was performed as part of the initial diagnostic evaluation in 35 patients with choroidal melanoma and no overt evidence of extraocular metastic diseases. The scans were normal in all but one patient in whom a solitary hepatic defect (due to an hemangioma) was found. The results of this study suggest that routine liver scans are not indicated in the initial staging evaluation of patients with choroidal melanoma, normal liver enzyme levels, and no other evidence of metastasis because of the low yield of scintigraphically detectable disease under these circumstances.", "contents": "The value of liver scintigraphy in choroidal melanoma. Radionuclide liver scintigraphy was performed as part of the initial diagnostic evaluation in 35 patients with choroidal melanoma and no overt evidence of extraocular metastic diseases. The scans were normal in all but one patient in whom a solitary hepatic defect (due to an hemangioma) was found. The results of this study suggest that routine liver scans are not indicated in the initial staging evaluation of patients with choroidal melanoma, normal liver enzyme levels, and no other evidence of metastasis because of the low yield of scintigraphically detectable disease under these circumstances."}
{"id": "PMID:485910", "title": "Latent, manifest latent, and congenital nystagmus.", "content": "Manifest latent nystagmus (MLN) was identified in 31 patients by accurate eye movement records. All the patients had horizontal tropias, and the nystagmus fast phases were always in the direction of viewing eye. The slow phases of MLN are decreasing-velocity exponentials while those of the jerk form of congenital nystagmus (CN) are increasing velocity exponentials. Several subjects who were believed to have latent nystagmus (LN) on clinical examination had small amplitude nystagmus evident by eye movement recording with both eyes open: these were actually cases of MLN. Many patients with MLN are misdiagnosed as having CN. An explanation for MLN and LN is provided based on confusion of egocentric directions between monocular and binocular viewing conditions.", "contents": "Latent, manifest latent, and congenital nystagmus. Manifest latent nystagmus (MLN) was identified in 31 patients by accurate eye movement records. All the patients had horizontal tropias, and the nystagmus fast phases were always in the direction of viewing eye. The slow phases of MLN are decreasing-velocity exponentials while those of the jerk form of congenital nystagmus (CN) are increasing velocity exponentials. Several subjects who were believed to have latent nystagmus (LN) on clinical examination had small amplitude nystagmus evident by eye movement recording with both eyes open: these were actually cases of MLN. Many patients with MLN are misdiagnosed as having CN. An explanation for MLN and LN is provided based on confusion of egocentric directions between monocular and binocular viewing conditions."}
{"id": "PMID:485911", "title": "Evaluation of Jensen procedures by saccades and diplopic fields.", "content": "A total of 15 eyes of 13 patients with lateral rectus palsies underwent Jensen procedures; one eye had a medial rectus recession and lateral rectus resection. It was arbitrarily decided to perform a Jensen procedure for patients with less than 40% normal abduction saccadic function. The patients' conditions were evaluated preoperatively and postoperatively by prism cover test. Versions, forced ductions, saccadic velocities, and diplopic field examinations were done. Postoperatively, all patients showed improved saccadic function; 12 of 13 patients acquired a functional range of diplopia-free vision; and 11 of 13 patients had aligned eyes with a good head position. By retrospective analysis we determined that the Jensen procedure gives good results for patients with severe lateral rectus palsy (less than 40% normal saccadic function).", "contents": "Evaluation of Jensen procedures by saccades and diplopic fields. A total of 15 eyes of 13 patients with lateral rectus palsies underwent Jensen procedures; one eye had a medial rectus recession and lateral rectus resection. It was arbitrarily decided to perform a Jensen procedure for patients with less than 40% normal abduction saccadic function. The patients' conditions were evaluated preoperatively and postoperatively by prism cover test. Versions, forced ductions, saccadic velocities, and diplopic field examinations were done. Postoperatively, all patients showed improved saccadic function; 12 of 13 patients acquired a functional range of diplopia-free vision; and 11 of 13 patients had aligned eyes with a good head position. By retrospective analysis we determined that the Jensen procedure gives good results for patients with severe lateral rectus palsy (less than 40% normal saccadic function)."}
{"id": "PMID:485912", "title": "Retinal wrinkling and macular heterotopia in diabetic retinopathy.", "content": "Retinal wrinkling of the posterior pole was studied in 40 eyes from 31 patients with diabetic retinopathy. Vitreoretinal traction was thought to produce the wrinkling in 32 of the eyes, in 13 of which macular heterotopia also developed, presumably from the same tractional forces. Epiretinal membrane formation was the apparent causal factor producing retinal wrinkling in six eyes and postphotocoagulation chorioretinal scarring in two eyes, in none of which macular heterotopia developed. Retinal wrinkling alone was associated with only mild or no visual acuity loss. Macular heterotopia was associated with more severe loss of visual acuity and with other visual symptoms. Displacement of retinal vessels was an important clue to the diagnosis of macular heterotopia.", "contents": "Retinal wrinkling and macular heterotopia in diabetic retinopathy. Retinal wrinkling of the posterior pole was studied in 40 eyes from 31 patients with diabetic retinopathy. Vitreoretinal traction was thought to produce the wrinkling in 32 of the eyes, in 13 of which macular heterotopia also developed, presumably from the same tractional forces. Epiretinal membrane formation was the apparent causal factor producing retinal wrinkling in six eyes and postphotocoagulation chorioretinal scarring in two eyes, in none of which macular heterotopia developed. Retinal wrinkling alone was associated with only mild or no visual acuity loss. Macular heterotopia was associated with more severe loss of visual acuity and with other visual symptoms. Displacement of retinal vessels was an important clue to the diagnosis of macular heterotopia."}
{"id": "PMID:485913", "title": "Electro-oculogram testing in fundus flavimaculatus.", "content": "Electro-oculogram (EOG) measurements were obtained on seven patients with moderately extensive lesions of fundus flavimaculatus. Each patient was tested by two different methods of measuring EOG light-peak to dark-trough ratios. The results show that erroneously low ratios can be observed unless a diffusing sphere is employed in the determination of light-peak to dark-trough ratios in fundus flavimaculatus and presumably other diseases that affect primarily the posterior pole.", "contents": "Electro-oculogram testing in fundus flavimaculatus. Electro-oculogram (EOG) measurements were obtained on seven patients with moderately extensive lesions of fundus flavimaculatus. Each patient was tested by two different methods of measuring EOG light-peak to dark-trough ratios. The results show that erroneously low ratios can be observed unless a diffusing sphere is employed in the determination of light-peak to dark-trough ratios in fundus flavimaculatus and presumably other diseases that affect primarily the posterior pole."}
{"id": "PMID:485914", "title": "Vision threshold profiles in sector retinitis pigmentosa.", "content": "Absolute vision thresholds were measured along the horizontal and vertical meridians in four patients from one pedigree and one patient from a second pedigree with dominantly inherited sector retinitis pigmentosa. From these studies we find that (1) visual function is decreased for all parts of the retina, including the fovea, (2) rod and cone systems are affected to the same degree, and (3) the disease progression is confined to the inferior portion of the retina.", "contents": "Vision threshold profiles in sector retinitis pigmentosa. Absolute vision thresholds were measured along the horizontal and vertical meridians in four patients from one pedigree and one patient from a second pedigree with dominantly inherited sector retinitis pigmentosa. From these studies we find that (1) visual function is decreased for all parts of the retina, including the fovea, (2) rod and cone systems are affected to the same degree, and (3) the disease progression is confined to the inferior portion of the retina."}
{"id": "PMID:485915", "title": "Aspiration cytodiagnosis of lid tumors.", "content": "In five patients, specimens of discrete, subepidermal lid tumors were successfully aspirated with 22- to 24-gauge spinal needles and dianosed cytologically. The simplicity, rapidity of diagnosis, and high accuracy of cytodiagnosis should make it a popular method for the evaluation of clinically puzzling subepidermal lid tumors.", "contents": "Aspiration cytodiagnosis of lid tumors. In five patients, specimens of discrete, subepidermal lid tumors were successfully aspirated with 22- to 24-gauge spinal needles and dianosed cytologically. The simplicity, rapidity of diagnosis, and high accuracy of cytodiagnosis should make it a popular method for the evaluation of clinically puzzling subepidermal lid tumors."}
{"id": "PMID:485916", "title": "Persistent pupillary membrane: successful medical management.", "content": "Persistent pupillary membranes usually undergo considerable atrophy during the first year of life. A minimum pupillary diameter of 1.5 mm is necessary for normal retinal image formation. Mydriatics, fixation pattern determination, and occlusion therapy as indicated can obviate the need for coreoplasty in patients with an extensive persistence of the pupillary membrane. We successfully treated a patient who had an almost total persistence of the pupillary membrane.", "contents": "Persistent pupillary membrane: successful medical management. Persistent pupillary membranes usually undergo considerable atrophy during the first year of life. A minimum pupillary diameter of 1.5 mm is necessary for normal retinal image formation. Mydriatics, fixation pattern determination, and occlusion therapy as indicated can obviate the need for coreoplasty in patients with an extensive persistence of the pupillary membrane. We successfully treated a patient who had an almost total persistence of the pupillary membrane."}
{"id": "PMID:485917", "title": "Laser iridotomy in closed-angle glaucoma.", "content": "An argon laser iridotomy was performed on 53 eyes of 34 patients with closed-angle glaucoma. Technically, all of the iridotomies were successful. Blue irides were more resistant to treatment than brown irides. The iridotomies were performed with high energy of 2 W and a very short exposure time of 0.01 to 0.04 s, thus minimizing thermal damage to adjacent tissues. Patients with narrow angles who underwent preventive laser irodotomy benefited most from the procedure, and all the eyes of this group maintained a normal intraocular pressure (IOP) after the iridotomy. Of the 20 eyes with acute angle-closure glaucoma, 17 maintained a normal IOP after iridotomy, and only three were resistant to this treatment, calling for filtering operations later. The IOP of eyes with chronic closed-angle glaucoma did not change after the procedure, and the iridocorneal angles remained practically the same. Argon laser iridotomy by this technique is highly recommended as the procedure of choice for eyes with acute angle-closure glaucoma and for preventive iridotomy in the fellow eye. The procedure is simple, is performed in the outpatient clinic, and engenders no major complications.", "contents": "Laser iridotomy in closed-angle glaucoma. An argon laser iridotomy was performed on 53 eyes of 34 patients with closed-angle glaucoma. Technically, all of the iridotomies were successful. Blue irides were more resistant to treatment than brown irides. The iridotomies were performed with high energy of 2 W and a very short exposure time of 0.01 to 0.04 s, thus minimizing thermal damage to adjacent tissues. Patients with narrow angles who underwent preventive laser irodotomy benefited most from the procedure, and all the eyes of this group maintained a normal intraocular pressure (IOP) after the iridotomy. Of the 20 eyes with acute angle-closure glaucoma, 17 maintained a normal IOP after iridotomy, and only three were resistant to this treatment, calling for filtering operations later. The IOP of eyes with chronic closed-angle glaucoma did not change after the procedure, and the iridocorneal angles remained practically the same. Argon laser iridotomy by this technique is highly recommended as the procedure of choice for eyes with acute angle-closure glaucoma and for preventive iridotomy in the fellow eye. The procedure is simple, is performed in the outpatient clinic, and engenders no major complications."}
{"id": "PMID:485918", "title": "Lateral incomitancy in intermittent exotropia: cause and surgical therapy.", "content": "A hypothesis, previously proposed, of tight medial rectus muscles in conjunction with tight lateral rectus muscles associated with exodeviations as a cause of lateral incomitancy in intermittent exotropia is supported by clinical management. Three patients with these findings underwent bilateral medial rectus and lateral rectus recessions by means of the adjustable rectus recession technique. Primary position alignment was achieved, and rotations were balanced with the alleviation of the lateral incomitancy. The lateral rectus muscles were recessed an amount more than usual in order to compensate for the recession of the medial rectus muscles.", "contents": "Lateral incomitancy in intermittent exotropia: cause and surgical therapy. A hypothesis, previously proposed, of tight medial rectus muscles in conjunction with tight lateral rectus muscles associated with exodeviations as a cause of lateral incomitancy in intermittent exotropia is supported by clinical management. Three patients with these findings underwent bilateral medial rectus and lateral rectus recessions by means of the adjustable rectus recession technique. Primary position alignment was achieved, and rotations were balanced with the alleviation of the lateral incomitancy. The lateral rectus muscles were recessed an amount more than usual in order to compensate for the recession of the medial rectus muscles."}
{"id": "PMID:485919", "title": "Influence of topically applied prazosin on the intraocular pressure of experimental animals.", "content": "Prazosin hydrochloride, an oral antihypertensive, is reported to inhibit phosphodiesterase and block postsynaptic alpha-adrenergic receptors, intraocular pressure (IOP) in rabbits was reduced in a dose-related manner following topical ocular application of concentrations of 0.0001% to 0.1%. The ocular hypotensive response lasted six to eight hours with a maximum effect at approximately two hours. The IOP of unilaterally sympathectomized rabbits treated in both eyes with prazosin decreased more in the normal eyes than in the sympathectomized eyes. Treatment of only the unsympathectomized eyes of these rabbits elicited a similar response. Normal rabbits elicited a similar response. Normal rabbits treated in only one eye also showed a slight response in the contralateral eye. Preliminary experiments did not support the hypothesis that these effects were due to a decrease of systemic blood pressure. No substantial ocular toxicity or pupillary changes were observed.", "contents": "Influence of topically applied prazosin on the intraocular pressure of experimental animals. Prazosin hydrochloride, an oral antihypertensive, is reported to inhibit phosphodiesterase and block postsynaptic alpha-adrenergic receptors, intraocular pressure (IOP) in rabbits was reduced in a dose-related manner following topical ocular application of concentrations of 0.0001% to 0.1%. The ocular hypotensive response lasted six to eight hours with a maximum effect at approximately two hours. The IOP of unilaterally sympathectomized rabbits treated in both eyes with prazosin decreased more in the normal eyes than in the sympathectomized eyes. Treatment of only the unsympathectomized eyes of these rabbits elicited a similar response. Normal rabbits elicited a similar response. Normal rabbits treated in only one eye also showed a slight response in the contralateral eye. Preliminary experiments did not support the hypothesis that these effects were due to a decrease of systemic blood pressure. No substantial ocular toxicity or pupillary changes were observed."}
{"id": "PMID:485920", "title": "Dipivefrin and echothiophate: contraindications to combined use.", "content": "A study was undertaken to determine whether the antiesterase activity of echothiophate iodide would prevent the conversion of dipivefrin to epinephrine. Dipivefrin was administered singly and in combination with echothiophate to 24 adult rabbits. Administration of dipivefrin lowered the intraocular pressure (IOP) 8 +/- 1 mm Hg (P less than .001). When echothiophate was given before and concomitant with dipivefrin, there was no further decrease in IOP compared with that produced by echothiophate alone (5 +/- 1 mm Hg). Addition of epinephrine to eyes receiving dipivefrin plus echothiophate resulted in a significant additional decrease in IOP of 4 +/- 1 mm Hg (P less than .001). When echothiophate was given after dipivefrin had lowered the IOP and both drugs were continued, the IOP rose to baseline levels. These results fit the theory that the esterase converting dipivefrin to epinephrine is inactivated by cholinesterase inhibitors. The clinical use of cholinesterase inhibitors and dipivefrin may be contraindicated.", "contents": "Dipivefrin and echothiophate: contraindications to combined use. A study was undertaken to determine whether the antiesterase activity of echothiophate iodide would prevent the conversion of dipivefrin to epinephrine. Dipivefrin was administered singly and in combination with echothiophate to 24 adult rabbits. Administration of dipivefrin lowered the intraocular pressure (IOP) 8 +/- 1 mm Hg (P less than .001). When echothiophate was given before and concomitant with dipivefrin, there was no further decrease in IOP compared with that produced by echothiophate alone (5 +/- 1 mm Hg). Addition of epinephrine to eyes receiving dipivefrin plus echothiophate resulted in a significant additional decrease in IOP of 4 +/- 1 mm Hg (P less than .001). When echothiophate was given after dipivefrin had lowered the IOP and both drugs were continued, the IOP rose to baseline levels. These results fit the theory that the esterase converting dipivefrin to epinephrine is inactivated by cholinesterase inhibitors. The clinical use of cholinesterase inhibitors and dipivefrin may be contraindicated."}
{"id": "PMID:485921", "title": "Kinetic vitreous fluorophotometry in experimental diabetes.", "content": "Kinetic vitreous fluorophotometry was used to measure dynamic alterations in blood-retinal barrier function. Normal hooded rats were compared with diabetic animals before and after insulin treatment. Rats with streptozocin-induced diabetes demonstrated significantly longer (P less than .001) half-periods of fluorescein loss from the vitreous when compared with controls, and insulin treatment significantly reduced (P less than .001) the mean half-period toward control values without normalization of serum glucose. These results suggest that kinetic vitreous fluorophotometry is a good indicator of blood-retinal barrier function and that sufficient amounts of insulin may be more important than normal blood glucose levels in recovery of altered barrier function in diabetes.", "contents": "Kinetic vitreous fluorophotometry in experimental diabetes. Kinetic vitreous fluorophotometry was used to measure dynamic alterations in blood-retinal barrier function. Normal hooded rats were compared with diabetic animals before and after insulin treatment. Rats with streptozocin-induced diabetes demonstrated significantly longer (P less than .001) half-periods of fluorescein loss from the vitreous when compared with controls, and insulin treatment significantly reduced (P less than .001) the mean half-period toward control values without normalization of serum glucose. These results suggest that kinetic vitreous fluorophotometry is a good indicator of blood-retinal barrier function and that sufficient amounts of insulin may be more important than normal blood glucose levels in recovery of altered barrier function in diabetes."}
{"id": "PMID:485922", "title": "Tear film breakup time prolonged with unmedicated cellulose polymer inserts.", "content": "Unmedicated cellulose polymer (UCP) inserts exhibited marked superiority over six commercial artificial tear substitutes in enhancing and maintaining precorneal tear film stability as measured by tear film breakup time (BUT) in rabbits. The application of UCP inserts into one eye also resulted in an appreciable elevation of the BUT in the contralateral untreated eye. Sham inserts (polyethylene pieces) equal in size to our larger than UCP inserts lacked similar effects, which suggests that the latter actions of UCP inserts are most likely due to a slow release of polymer into the tear film rather than to induction of reflex responses associated with insertion of an inert foreign body into the cul-de-sac. The results indicate that UCP inserts, by virtue of their ability to maintain the integrity of the precorneal tear film over extended periods of time, may represent an important advance in the treatment of dry-eye diseases.", "contents": "Tear film breakup time prolonged with unmedicated cellulose polymer inserts. Unmedicated cellulose polymer (UCP) inserts exhibited marked superiority over six commercial artificial tear substitutes in enhancing and maintaining precorneal tear film stability as measured by tear film breakup time (BUT) in rabbits. The application of UCP inserts into one eye also resulted in an appreciable elevation of the BUT in the contralateral untreated eye. Sham inserts (polyethylene pieces) equal in size to our larger than UCP inserts lacked similar effects, which suggests that the latter actions of UCP inserts are most likely due to a slow release of polymer into the tear film rather than to induction of reflex responses associated with insertion of an inert foreign body into the cul-de-sac. The results indicate that UCP inserts, by virtue of their ability to maintain the integrity of the precorneal tear film over extended periods of time, may represent an important advance in the treatment of dry-eye diseases."}
{"id": "PMID:485923", "title": "Dextran's effects on stressed lenses: water, electrolyte, and radioisotope studies.", "content": "To evaluate the beneficial effects of dextran 40 as an additive to infusion solutions, we studied an experimental model of lens stress with use of buffered, low calcium (Ca++)-containing solutions. Incubation in low Ca++ solutions (pCa = 10.7) for ten hours (stress period) resulted in lens swelling and electrolyte imbalances that were irreversible even with reincubation in physiologic, normal Ca++-containing media (pCa = 2.7) (recovery period). The addition of 6% or more of dextran to the media inhibited lens water gain during the stress period. It also rendered the resultant electrolyte imbalances reversible during the recovery period, thus exerting a protective effect. Radioisotope-tracer studies showed that dextran improved the ability of the lens to accumulate rubidium chloride Rb 86 and reduced its efflux during both the stress and recovery periods. Although dextran did not markedly decrease sodium chloride Na 22 uptake by lenses under stress, it did allow the lens to remove the 22Na during the recovery period.", "contents": "Dextran's effects on stressed lenses: water, electrolyte, and radioisotope studies. To evaluate the beneficial effects of dextran 40 as an additive to infusion solutions, we studied an experimental model of lens stress with use of buffered, low calcium (Ca++)-containing solutions. Incubation in low Ca++ solutions (pCa = 10.7) for ten hours (stress period) resulted in lens swelling and electrolyte imbalances that were irreversible even with reincubation in physiologic, normal Ca++-containing media (pCa = 2.7) (recovery period). The addition of 6% or more of dextran to the media inhibited lens water gain during the stress period. It also rendered the resultant electrolyte imbalances reversible during the recovery period, thus exerting a protective effect. Radioisotope-tracer studies showed that dextran improved the ability of the lens to accumulate rubidium chloride Rb 86 and reduced its efflux during both the stress and recovery periods. Although dextran did not markedly decrease sodium chloride Na 22 uptake by lenses under stress, it did allow the lens to remove the 22Na during the recovery period."}
{"id": "PMID:485927", "title": "Rhomboid flap in facial reconstruction. New concept of tension lines.", "content": "The rhomboid flap has become a popular reconstructive alternative for facial defects in recent years. Since the original description by Limberg, several modifications of the flap have been described, the most important of which was by Dufourmental. In the literature and at many soft-tissue courses, it has been taught that after rotation the maximum tension of the rhomboid flap is at the corner of the rhomboid most distant from the transposed flap. The author shows that the maximum tension of the flap is at the closure of the donor area. This phenomenon is amply illustrated with clinical cases. The Dufourmental modification is more satisfactory than the classical rhomboid flap in most cases because there is less tension on the closure. An understanding of these concepts is essential to the successful use of this important flap in order to avoid distortion of vital facial landmarks. I report a series of 30 consecutive rhomboid flaps.", "contents": "Rhomboid flap in facial reconstruction. New concept of tension lines. The rhomboid flap has become a popular reconstructive alternative for facial defects in recent years. Since the original description by Limberg, several modifications of the flap have been described, the most important of which was by Dufourmental. In the literature and at many soft-tissue courses, it has been taught that after rotation the maximum tension of the rhomboid flap is at the corner of the rhomboid most distant from the transposed flap. The author shows that the maximum tension of the flap is at the closure of the donor area. This phenomenon is amply illustrated with clinical cases. The Dufourmental modification is more satisfactory than the classical rhomboid flap in most cases because there is less tension on the closure. An understanding of these concepts is essential to the successful use of this important flap in order to avoid distortion of vital facial landmarks. I report a series of 30 consecutive rhomboid flaps."}
{"id": "PMID:485928", "title": "The omocervical flap.", "content": "The omocervical flap is recommended for replacement of neck skin involved with malignancy or radioecrosis, carotid artery protection, or closure of pharyngostomes. The flap is very strong and reliable. It has a permanent pedicle and can be turned without delay. It will reach beyond the midline. By using appropriate lateral positioning of the patient, neck surgery and flap transfer can be accomplished with minimum difficulty. This flap is recommended as an excellent functional and cosmetic flap for neck reconstruction.", "contents": "The omocervical flap. The omocervical flap is recommended for replacement of neck skin involved with malignancy or radioecrosis, carotid artery protection, or closure of pharyngostomes. The flap is very strong and reliable. It has a permanent pedicle and can be turned without delay. It will reach beyond the midline. By using appropriate lateral positioning of the patient, neck surgery and flap transfer can be accomplished with minimum difficulty. This flap is recommended as an excellent functional and cosmetic flap for neck reconstruction."}
{"id": "PMID:485929", "title": "Synchronous multiple primary lesions of the upper aerodigestive system.", "content": "Multiple primary lesions were initially thought to be rare occurrences; however, recent numerous series show the incidence to vary from 1% to 11%. These reports generally refer to lesions that develop subsequently to diagnosis and treatment of the initial lesion, and the results of these studies reflect improved follow-up, treatment, and possibly a true increase in frequency. In the search for synchronously developing primary lesions, it was decided to perform panendoscopies on all cases of cancer of the upper aerodigestive tract, irrespective of size or situation or whether they were amenable to evaluation under local anesthesia. These panendoscopies were performed on 162 consecutive cases with a diagnosis of 15 synchronous second primary lesions, ie, an incidence of 9.2%. For this reason, as much time and effort as possible should be devoted to detecting the synchronous second primary lesion as the later developing lesion, and panendoscopy has proved invaluable in achieving this.", "contents": "Synchronous multiple primary lesions of the upper aerodigestive system. Multiple primary lesions were initially thought to be rare occurrences; however, recent numerous series show the incidence to vary from 1% to 11%. These reports generally refer to lesions that develop subsequently to diagnosis and treatment of the initial lesion, and the results of these studies reflect improved follow-up, treatment, and possibly a true increase in frequency. In the search for synchronously developing primary lesions, it was decided to perform panendoscopies on all cases of cancer of the upper aerodigestive tract, irrespective of size or situation or whether they were amenable to evaluation under local anesthesia. These panendoscopies were performed on 162 consecutive cases with a diagnosis of 15 synchronous second primary lesions, ie, an incidence of 9.2%. For this reason, as much time and effort as possible should be devoted to detecting the synchronous second primary lesion as the later developing lesion, and panendoscopy has proved invaluable in achieving this."}
{"id": "PMID:485930", "title": "Revision of the endolymphatic subarachnoid shunt for Meniere's disease. Review of 59 cases.", "content": "Members of the Otologic Medical Group, Inc, Los Angeles, diagnose Meniere's disease in patients with fluctuating hearing loss, fluctuating tinnitus, episodic vertigo, and fluctuating pressure symptoms in the ear. Treatment is vasodilation. If medical treatment fails, an endolymphatic subarachnoid shunt operation is performed. If the shunt relieves symptoms for three or more months but symptoms then recur, revision of the shunt is recommended. If the revision surgery also fails, a destructive procedure is suggested.", "contents": "Revision of the endolymphatic subarachnoid shunt for Meniere's disease. Review of 59 cases. Members of the Otologic Medical Group, Inc, Los Angeles, diagnose Meniere's disease in patients with fluctuating hearing loss, fluctuating tinnitus, episodic vertigo, and fluctuating pressure symptoms in the ear. Treatment is vasodilation. If medical treatment fails, an endolymphatic subarachnoid shunt operation is performed. If the shunt relieves symptoms for three or more months but symptoms then recur, revision of the shunt is recommended. If the revision surgery also fails, a destructive procedure is suggested."}
{"id": "PMID:485931", "title": "A study of the practice of otorhinolaryngology in the United States. Initial findings.", "content": "Results of a national survey of nonfederal otorhinolaryngologist regarding their practices provide the following portrait of the typical physician who specializes in otorhinolaryngology: male, between 35 and 45 years old, engaged in solo practice in a metropolitan area in the southern part of the country. He works a 46-hour week, devoting most of his time to patient care. During a typical week, he sees 113 patients, mostly outside the hospital. Half of his patients are male; although their average age is 35, one fourth of his patients are under 15 years. Every third patient is new to him; every other patient has been referred. He spends almost 15 minutes with each patient, many of whom he is seeing on a continuous basis for a problem involving the ear. He prescribes medications in roughly half of all encounters; every tenth patient requires surgery. He rates the majority of the problems he encounters as chronic, and of minor to moderate severity and urgency--for which he provides services of limited complexity or less. He seldom refers his patients.", "contents": "A study of the practice of otorhinolaryngology in the United States. Initial findings. Results of a national survey of nonfederal otorhinolaryngologist regarding their practices provide the following portrait of the typical physician who specializes in otorhinolaryngology: male, between 35 and 45 years old, engaged in solo practice in a metropolitan area in the southern part of the country. He works a 46-hour week, devoting most of his time to patient care. During a typical week, he sees 113 patients, mostly outside the hospital. Half of his patients are male; although their average age is 35, one fourth of his patients are under 15 years. Every third patient is new to him; every other patient has been referred. He spends almost 15 minutes with each patient, many of whom he is seeing on a continuous basis for a problem involving the ear. He prescribes medications in roughly half of all encounters; every tenth patient requires surgery. He rates the majority of the problems he encounters as chronic, and of minor to moderate severity and urgency--for which he provides services of limited complexity or less. He seldom refers his patients."}
{"id": "PMID:485935", "title": "Noise and cochlear blood flow.", "content": "The effect of noise after unilateral transection of the cervical sympathetic trunk on cochlear blood flow was studied in anesthetized cats. The sound pressure level was 100 dB and the exposure time 6 min. Neither noise nor sympathectomy were found to affect the blood flow.", "contents": "Noise and cochlear blood flow. The effect of noise after unilateral transection of the cervical sympathetic trunk on cochlear blood flow was studied in anesthetized cats. The sound pressure level was 100 dB and the exposure time 6 min. Neither noise nor sympathectomy were found to affect the blood flow."}
{"id": "PMID:485936", "title": "Acoustic trauma effects from doubling the exposure time.", "content": "Guinea pigs were exposed to 20 kHz for a period of 1 or 2 h, with and without an intermediate recovery period. Statistically smaller areas of damage were obtained after doubling the exposure time, especially without a recovery period. For a double exposure with a recovery period the areas of damage were the same as for the single exposures.", "contents": "Acoustic trauma effects from doubling the exposure time. Guinea pigs were exposed to 20 kHz for a period of 1 or 2 h, with and without an intermediate recovery period. Statistically smaller areas of damage were obtained after doubling the exposure time, especially without a recovery period. For a double exposure with a recovery period the areas of damage were the same as for the single exposures."}
{"id": "PMID:485937", "title": "Auditory nerve activity and cochlear morphology after noise exposure.", "content": "Four chinchillas were exposed for 5 days to an octave band of noise centered at 4 kHz and having an SPL of 86 dB. After a recovery period of approximately 6 months, behavioral audiograms were obtained and auditory nerve fiber activity was recorded. The animals were killed and the cochleas embedded in plastic to obtain a surface preparation and 1 mu radial sections of the organ of Corti. Behavioral threshold shifts ranged from 5 to 20 dB at frequencies between 4 and 11 kHz. Auditory nerve fiber thresholds were elevated up to 70 dB for units with characteristic frequencies between 4 and 14 kHz. Units with higher and lower characteristic frequencies had normal thresholds. Cochleagrams showed narrow lesions of inner and/or outer hair cells over approximately a 1 mm distance. A comparison of the three realms of data revealed the following: (1) The greatest threshold shifts from the noise exposure were seen in the single nerve fiber thresholds while the smallest shifts were seen in the behavioral thresholds, (2) the greatest behavioral and neural threshold shifts and greatest cochlear damage occurred 1 octave above the center frequency of the noise exposure, and (3) based on the frequency-place map of the chinchilla cochlea, the range of fibers with elevated thresholds exceeded the extent of the OHC lesion. A number of anatomical changes were seen that effectively increased the extent of the damage found in the chochleagram. These changes included: distortions in the surface topography of the organ of Corti affecting the orientation of IHC; missing pillar cells in the presence of normal OHC and/or IHC and protrusion of the IHC cuticular plate into the subtectorial space.", "contents": "Auditory nerve activity and cochlear morphology after noise exposure. Four chinchillas were exposed for 5 days to an octave band of noise centered at 4 kHz and having an SPL of 86 dB. After a recovery period of approximately 6 months, behavioral audiograms were obtained and auditory nerve fiber activity was recorded. The animals were killed and the cochleas embedded in plastic to obtain a surface preparation and 1 mu radial sections of the organ of Corti. Behavioral threshold shifts ranged from 5 to 20 dB at frequencies between 4 and 11 kHz. Auditory nerve fiber thresholds were elevated up to 70 dB for units with characteristic frequencies between 4 and 14 kHz. Units with higher and lower characteristic frequencies had normal thresholds. Cochleagrams showed narrow lesions of inner and/or outer hair cells over approximately a 1 mm distance. A comparison of the three realms of data revealed the following: (1) The greatest threshold shifts from the noise exposure were seen in the single nerve fiber thresholds while the smallest shifts were seen in the behavioral thresholds, (2) the greatest behavioral and neural threshold shifts and greatest cochlear damage occurred 1 octave above the center frequency of the noise exposure, and (3) based on the frequency-place map of the chinchilla cochlea, the range of fibers with elevated thresholds exceeded the extent of the OHC lesion. A number of anatomical changes were seen that effectively increased the extent of the damage found in the chochleagram. These changes included: distortions in the surface topography of the organ of Corti affecting the orientation of IHC; missing pillar cells in the presence of normal OHC and/or IHC and protrusion of the IHC cuticular plate into the subtectorial space."}
{"id": "PMID:485938", "title": "Effects of acute noise traumata on whole-nerve and single-unit activity.", "content": "This paper gives some preliminary results of a research program on the effects of noise traumata on cochlear functioning. Measurements reported here include: the latency of the whole-nerve (compound) action potential (AP), the selectivity of frequency-tuning curves (e.g., the Q10-values) measured for the AP (with a forward-masking technique) and for single units in the antero-ventral cochlear nucleus (AVCN), the degree of phase-locking of the action potentials of these units to the stimulus waveform, and the phase of the stimulus waveform to which these potentials are locking. The noise traumata were induced during the experiments. After inducement of the traumata we found: an increase of AP latency, if a sinusoidal stimulas was presented at the same sound pressure level as before the trauma; no effect on AP latency (for most stimulus frequencies), if the latency values measured at the pre- and post-trauma thresholds were compared; a decrease of Q10-values of the AP frequency-tuning curves, (the pre-trauma Q10-values were small in comparison with single nerve fiber data); a decrease of the Q10-values of the single-unit frequency-tuning curves; no effect on the degree of phase-locking of the single-unit potentials to the stimulus waveform; an increase of cochlear response time calculated from the phase of the stimulus waveform to which the single-unit potentials are locking as a function of stimulus frequency.", "contents": "Effects of acute noise traumata on whole-nerve and single-unit activity. This paper gives some preliminary results of a research program on the effects of noise traumata on cochlear functioning. Measurements reported here include: the latency of the whole-nerve (compound) action potential (AP), the selectivity of frequency-tuning curves (e.g., the Q10-values) measured for the AP (with a forward-masking technique) and for single units in the antero-ventral cochlear nucleus (AVCN), the degree of phase-locking of the action potentials of these units to the stimulus waveform, and the phase of the stimulus waveform to which these potentials are locking. The noise traumata were induced during the experiments. After inducement of the traumata we found: an increase of AP latency, if a sinusoidal stimulas was presented at the same sound pressure level as before the trauma; no effect on AP latency (for most stimulus frequencies), if the latency values measured at the pre- and post-trauma thresholds were compared; a decrease of Q10-values of the AP frequency-tuning curves, (the pre-trauma Q10-values were small in comparison with single nerve fiber data); a decrease of the Q10-values of the single-unit frequency-tuning curves; no effect on the degree of phase-locking of the single-unit potentials to the stimulus waveform; an increase of cochlear response time calculated from the phase of the stimulus waveform to which the single-unit potentials are locking as a function of stimulus frequency."}
{"id": "PMID:485939", "title": "Influence of thyroid state and improved hypoxia tolerance on noise-induced cochlea damage.", "content": "Guinea pigs were exposed to pure tone noise (2.7 kHz, 130 dB, 1 h) and cochlear microphonic potentials were measured 24 h after exposure. There is the possibility to modify the resulting noise-induced cochlea damage by regulating the function of the thyroid gland to alter the rate of metabolism. A hypofunction of the thyroid gland during sound exposure lessens, an over-function aggravates the damage. After gradual adaptation of the animals to a simulated 10,000 m altitude, the electrophysiologically demonstratable noise-induced damage was reduced. This might be explained by the greater hypoxia tolerance and perhaps additional better oxygen supply to the receptor cells.", "contents": "Influence of thyroid state and improved hypoxia tolerance on noise-induced cochlea damage. Guinea pigs were exposed to pure tone noise (2.7 kHz, 130 dB, 1 h) and cochlear microphonic potentials were measured 24 h after exposure. There is the possibility to modify the resulting noise-induced cochlea damage by regulating the function of the thyroid gland to alter the rate of metabolism. A hypofunction of the thyroid gland during sound exposure lessens, an over-function aggravates the damage. After gradual adaptation of the animals to a simulated 10,000 m altitude, the electrophysiologically demonstratable noise-induced damage was reduced. This might be explained by the greater hypoxia tolerance and perhaps additional better oxygen supply to the receptor cells."}
{"id": "PMID:485941", "title": "Auditory function in guinea pigs treated with netilmicin and other aminoglycoside antibiotics.", "content": "The following aminoglycoside antibiotics netilmicin, sisomicin, gentamicin, and kanamycin were submitted to a comparative study of their ototoxicity using both reflexological (Preyer's pinnareflex) and electrophysiological (near and far field) methods. The daily s.c. administration of sisomicin, gentamicin, and kanamycin for 21 days provoked a dose-related impairment of the cochlear function, detected with all the employed techniques. On the other hand, a very low ototoxic effect of netilmicin was demonstrated with electrophysiological but not with the reflexological evaluation. The reliability of the methods used in these experiments is also compared.", "contents": "Auditory function in guinea pigs treated with netilmicin and other aminoglycoside antibiotics. The following aminoglycoside antibiotics netilmicin, sisomicin, gentamicin, and kanamycin were submitted to a comparative study of their ototoxicity using both reflexological (Preyer's pinnareflex) and electrophysiological (near and far field) methods. The daily s.c. administration of sisomicin, gentamicin, and kanamycin for 21 days provoked a dose-related impairment of the cochlear function, detected with all the employed techniques. On the other hand, a very low ototoxic effect of netilmicin was demonstrated with electrophysiological but not with the reflexological evaluation. The reliability of the methods used in these experiments is also compared."}
{"id": "PMID:485942", "title": "Effects of modern loop diuretics on the inner ear a quantitative evaluation using computer technics.", "content": "The effects of some modern high ceiling loop diuretics on the guinea-pig's inner ear are tested. Short-time experiments are run registrating the cochlear microphonic potentials (CMP) before and after i.v. bolus injections of a diuretic. A 100-point matrix program is performed by automatic sampling of the CMPs after stimulating by five frequencies at 20 sound pressure levels each. Data can be plotted in different scales within 10 min after the end of a whole-day experiment. The data are on-line processed by a IBM 1800 computer.", "contents": "Effects of modern loop diuretics on the inner ear a quantitative evaluation using computer technics. The effects of some modern high ceiling loop diuretics on the guinea-pig's inner ear are tested. Short-time experiments are run registrating the cochlear microphonic potentials (CMP) before and after i.v. bolus injections of a diuretic. A 100-point matrix program is performed by automatic sampling of the CMPs after stimulating by five frequencies at 20 sound pressure levels each. Data can be plotted in different scales within 10 min after the end of a whole-day experiment. The data are on-line processed by a IBM 1800 computer."}
{"id": "PMID:485943", "title": "Cell membrane alterations in the stria vascularis of the guinea pig after ethacrynic acid treatment studied by freeze-fracture.", "content": "A freeze-fracture examination of the stria vascularis during the first 2 h after injection of ethacrynic acid was performed. This showed a re-distribution of the particles on the membrane fracture faces of both marginal and intermediate cells. As oedematous spaces developed, particle-poor, vesicle-like structures were found associated with both cell types. The tight junctions at the apices of the marginal cells and around basal cells were unaffected.", "contents": "Cell membrane alterations in the stria vascularis of the guinea pig after ethacrynic acid treatment studied by freeze-fracture. A freeze-fracture examination of the stria vascularis during the first 2 h after injection of ethacrynic acid was performed. This showed a re-distribution of the particles on the membrane fracture faces of both marginal and intermediate cells. As oedematous spaces developed, particle-poor, vesicle-like structures were found associated with both cell types. The tight junctions at the apices of the marginal cells and around basal cells were unaffected."}
{"id": "PMID:485945", "title": "The inner ear and the in vitro system.", "content": "The embryologic labyrinthine development of the CBA/CBA mouse occurs parallell in vivo and in vitro. Regarding post partum inner ears, either as cultured otocysts passing a corresponding time in vitro or inner ear explants of newborn/mature animals, the extracorporal system becomes unable to maintain specialized hair cell structures for more than a few days. The sensory cells themselves, however, survive for considerably longer time. Vestibular hair cells show sensory hair fusion. Cochlear hair cells loose their surface structures but the sensory hair rootlets penetrating into the cuticle are preserved. Post partum inner ears from the guinea pig reacted in a similar way in vitro as did labyrinths from the CBA/CBA mouse.", "contents": "The inner ear and the in vitro system. The embryologic labyrinthine development of the CBA/CBA mouse occurs parallell in vivo and in vitro. Regarding post partum inner ears, either as cultured otocysts passing a corresponding time in vitro or inner ear explants of newborn/mature animals, the extracorporal system becomes unable to maintain specialized hair cell structures for more than a few days. The sensory cells themselves, however, survive for considerably longer time. Vestibular hair cells show sensory hair fusion. Cochlear hair cells loose their surface structures but the sensory hair rootlets penetrating into the cuticle are preserved. Post partum inner ears from the guinea pig reacted in a similar way in vitro as did labyrinths from the CBA/CBA mouse."}
{"id": "PMID:485946", "title": "Localization of the marginal zone of the tectorial membrane in situ, unfixed, and with in vivo-like ionic milieu.", "content": "In order to determine the normal positional relationship between the tectorial membrane and the organ of Corti, a preparation method was developed which made it possible to study the unfixed tectorial membrane in its normal position in relation to the cochlea and with in vivo-like ionic conditions. With this method, post-mortem changes visible with the light microscope were detectable after 60 to 90 min instead of the normal 30 min. When endolymph of artifical endolymph are present in the scala media, the marginal zone lies in close contact with the surface of the organ of Corti. If the endolymph is replaced by artificial perilymph, first the marginal zone of the tectorial membrane, and later the whole membrane shrinks. At this stage, latex particles suspended in the perilymph are free to enter the subtectorial space.", "contents": "Localization of the marginal zone of the tectorial membrane in situ, unfixed, and with in vivo-like ionic milieu. In order to determine the normal positional relationship between the tectorial membrane and the organ of Corti, a preparation method was developed which made it possible to study the unfixed tectorial membrane in its normal position in relation to the cochlea and with in vivo-like ionic conditions. With this method, post-mortem changes visible with the light microscope were detectable after 60 to 90 min instead of the normal 30 min. When endolymph of artifical endolymph are present in the scala media, the marginal zone lies in close contact with the surface of the organ of Corti. If the endolymph is replaced by artificial perilymph, first the marginal zone of the tectorial membrane, and later the whole membrane shrinks. At this stage, latex particles suspended in the perilymph are free to enter the subtectorial space."}
{"id": "PMID:485947", "title": "Functions of different receptor systems in the reptilian labyrinth.", "content": "Different biopotentials, following a mechanical stimulus, were recorded from single hair cells of the reptilian labyrinth, with electronoptically circumscript localizations: 1. From the apical cell pole, the receptor potential, intracellular or from the ciliary surface, within the physiological range proportional to stimulus amplitude, frequency or phase, without delay, and with no real threshold of mechanosensitivity, as measured by ciliary displacement amplitude or velocity. 2. From the synaptic zone, in the basal region of the hair cell, or from contacting nerve endings, the synaptic potentials, local excitatory or inhibitory processes, respectively, with measurable latencies and with non-linear distortion. 3. From the (dendritic) endings of the first afferent neuron (or neurons), spike-shaped action potentials, synchronized by the (excitatory) synaptic potentials. Characteristic curves were plotted as a quantitative representation of the mechano-electric input-output relations of the different types of hair cells. For proceeding morphological and physiological system analysis, the comparison of the different submammalian inner-ear receptor systems gives us some new possibility of a closer correlation between ultrastructure and function.", "contents": "Functions of different receptor systems in the reptilian labyrinth. Different biopotentials, following a mechanical stimulus, were recorded from single hair cells of the reptilian labyrinth, with electronoptically circumscript localizations: 1. From the apical cell pole, the receptor potential, intracellular or from the ciliary surface, within the physiological range proportional to stimulus amplitude, frequency or phase, without delay, and with no real threshold of mechanosensitivity, as measured by ciliary displacement amplitude or velocity. 2. From the synaptic zone, in the basal region of the hair cell, or from contacting nerve endings, the synaptic potentials, local excitatory or inhibitory processes, respectively, with measurable latencies and with non-linear distortion. 3. From the (dendritic) endings of the first afferent neuron (or neurons), spike-shaped action potentials, synchronized by the (excitatory) synaptic potentials. Characteristic curves were plotted as a quantitative representation of the mechano-electric input-output relations of the different types of hair cells. For proceeding morphological and physiological system analysis, the comparison of the different submammalian inner-ear receptor systems gives us some new possibility of a closer correlation between ultrastructure and function."}
{"id": "PMID:485948", "title": "Evidence of mechanical nonlinearity and frequency selective wave amplification in the cochlea.", "content": "A previously unsuspected mechanical response of the cochlear transduction mechanism has recently been discovered. It can be monitored outside the cochlear. Theorectical and experimental work leading to its discovery and identification are briefly reviewed. Its significance for models of the second filter and cochlear nonlinearity is discussed. An active wave amplification mechanism in the cochlea is proposed.", "contents": "Evidence of mechanical nonlinearity and frequency selective wave amplification in the cochlea. A previously unsuspected mechanical response of the cochlear transduction mechanism has recently been discovered. It can be monitored outside the cochlear. Theorectical and experimental work leading to its discovery and identification are briefly reviewed. Its significance for models of the second filter and cochlear nonlinearity is discussed. An active wave amplification mechanism in the cochlea is proposed."}
{"id": "PMID:485949", "title": "Local cochlear masking by frequency-modulated tone bursts.", "content": "Masking experiments are demonstrated for electrical frequency-modulated tone bursts from 1,000 to 10,000 cps and from 10,000 to 1,000 cps with superimposed clicks. The maximum of the masking was found at 3,700 cps (\"narrow-band masking\").", "contents": "Local cochlear masking by frequency-modulated tone bursts. Masking experiments are demonstrated for electrical frequency-modulated tone bursts from 1,000 to 10,000 cps and from 10,000 to 1,000 cps with superimposed clicks. The maximum of the masking was found at 3,700 cps (\"narrow-band masking\")."}
{"id": "PMID:485950", "title": "\"Neural\" responses to acoustic stimulation after destruction of cochlear hair cells.", "content": "Electrophysiological and histological observations in guinea pig's cochleas after amikacin treatment (14 X 450 mg/kg) confirm the results obtained in a former experiment: clear, short-latency, click-evoked responses were recorded in cochleas with only very few hair cells remaining at the extreme apex. Detailed analysis of these responses strongly indicates a neural origin and confirms their low-frequency sensitivity. Careful histological observations confirm the extensive hair cell loss and the preservation of nerve fibers in the remnants of the organ of Corti and of the vestibular sense organs. These results suggest that the acoustical vibrations either stimulate the vestibular receptors or act directly or through some kind of mechano-electrical transduction on the remaining cochlear nerve fibers.", "contents": "\"Neural\" responses to acoustic stimulation after destruction of cochlear hair cells. Electrophysiological and histological observations in guinea pig's cochleas after amikacin treatment (14 X 450 mg/kg) confirm the results obtained in a former experiment: clear, short-latency, click-evoked responses were recorded in cochleas with only very few hair cells remaining at the extreme apex. Detailed analysis of these responses strongly indicates a neural origin and confirms their low-frequency sensitivity. Careful histological observations confirm the extensive hair cell loss and the preservation of nerve fibers in the remnants of the organ of Corti and of the vestibular sense organs. These results suggest that the acoustical vibrations either stimulate the vestibular receptors or act directly or through some kind of mechano-electrical transduction on the remaining cochlear nerve fibers."}
{"id": "PMID:485951", "title": "Some aspects of temporal coding by single cochlear fibres from regions of cochlear hair cell degeneration in the guinea pig.", "content": "Some temporal coding properties of cochlear nerve fibers are investigated in kanamycin-treated guinea pigs (GPs) with various degrees of outer hair cell (OHC) degeneration. In particular, the phase locking ability of fibres from pathological cochleas, and also their adaptation properties are compared with the properties of normal cochlear fibres. No systematic effects of OHC loss on these properties have so far been found. These preliminary results therefore suggest (in so far as these animals can be regarded as models of sensorineural hearing loss of cochlear origin in man) that little deterioration should be expected in functions purely dependent upon faithful temporal coding of the stimulus waveform.", "contents": "Some aspects of temporal coding by single cochlear fibres from regions of cochlear hair cell degeneration in the guinea pig. Some temporal coding properties of cochlear nerve fibers are investigated in kanamycin-treated guinea pigs (GPs) with various degrees of outer hair cell (OHC) degeneration. In particular, the phase locking ability of fibres from pathological cochleas, and also their adaptation properties are compared with the properties of normal cochlear fibres. No systematic effects of OHC loss on these properties have so far been found. These preliminary results therefore suggest (in so far as these animals can be regarded as models of sensorineural hearing loss of cochlear origin in man) that little deterioration should be expected in functions purely dependent upon faithful temporal coding of the stimulus waveform."}
{"id": "PMID:485952", "title": "Grafting and electrical stimulation of the auditory nerve in cats.", "content": "The effect of implanting wire electrodes into the cochlea is limited by the deficit of nerve fibers in most diseases of the cochlea. Therefore, in a series of experiments it was tried, for the first time, to direct new outspreading nerve fibres into the afferent acoustic nerve by grafting a motor nerve to it. A second series of experiments should demonstrate the possibility of direct electrical stimulation of the eighs nerve by implanting wire electrodes via the transoccipital approach. The electrically evoked potentials, recorded at the auditory cortex, were compaired to potentials evoked by auditory stimulation. The answers to acoustic or electric stimulation showed no significant difference.", "contents": "Grafting and electrical stimulation of the auditory nerve in cats. The effect of implanting wire electrodes into the cochlea is limited by the deficit of nerve fibers in most diseases of the cochlea. Therefore, in a series of experiments it was tried, for the first time, to direct new outspreading nerve fibres into the afferent acoustic nerve by grafting a motor nerve to it. A second series of experiments should demonstrate the possibility of direct electrical stimulation of the eighs nerve by implanting wire electrodes via the transoccipital approach. The electrically evoked potentials, recorded at the auditory cortex, were compaired to potentials evoked by auditory stimulation. The answers to acoustic or electric stimulation showed no significant difference."}
{"id": "PMID:485953", "title": "The permeability of 51Cr between cerebrospinal fluid and internal ear.", "content": "Radioactively labelled chromium was instilled in the cerebrospinal fluid of guinea pigs. After different intervals, the radioactivity of inner ear fluids was measured and compared with those of other regions. The results lead to kinetic information about the permeability of 51Cr between cerebrospinal and inner ear fluids.", "contents": "The permeability of 51Cr between cerebrospinal fluid and internal ear. Radioactively labelled chromium was instilled in the cerebrospinal fluid of guinea pigs. After different intervals, the radioactivity of inner ear fluids was measured and compared with those of other regions. The results lead to kinetic information about the permeability of 51Cr between cerebrospinal and inner ear fluids."}
{"id": "PMID:485954", "title": "Adenine nucleotides of the stria vascularis.", "content": "The levels of the adenine nucleotides ATP, ADP, and AMP in the stria vascularis were measured under normal conditions, and following various durations of ischemia. The concentrations of these compounds were used for the calculation of the adenylate energy charge, the energy status and the phosphorylation state of the stria. Following 10 min of ischemia the adenylate energy charge had decreased three fold, the energy status seven fold and the phosphorylation state 14 fold. To study the potential for recovery of strial function following various brief and prolonged ischemic intervals, a method for the perfusion of the ear via the anterior inferior cerebellar artery was developed. For various reasons it was found advantageous to use \"artifical blood\" as perfusate, relying upon fluorocarbons as oxygen carriers. The endolymphatic potential was used as electrical indicator of strial function. Recovery of the endolymphatic potential following brief periods of ischemia was paralleled by a corresponding increase of the ATP levels and a drastic decrease of the AMP levels of the stria vascularis. Preliminary results on the effects of substrate-free perfusion are presented.", "contents": "Adenine nucleotides of the stria vascularis. The levels of the adenine nucleotides ATP, ADP, and AMP in the stria vascularis were measured under normal conditions, and following various durations of ischemia. The concentrations of these compounds were used for the calculation of the adenylate energy charge, the energy status and the phosphorylation state of the stria. Following 10 min of ischemia the adenylate energy charge had decreased three fold, the energy status seven fold and the phosphorylation state 14 fold. To study the potential for recovery of strial function following various brief and prolonged ischemic intervals, a method for the perfusion of the ear via the anterior inferior cerebellar artery was developed. For various reasons it was found advantageous to use \"artifical blood\" as perfusate, relying upon fluorocarbons as oxygen carriers. The endolymphatic potential was used as electrical indicator of strial function. Recovery of the endolymphatic potential following brief periods of ischemia was paralleled by a corresponding increase of the ATP levels and a drastic decrease of the AMP levels of the stria vascularis. Preliminary results on the effects of substrate-free perfusion are presented."}
{"id": "PMID:485955", "title": "Some vascular effects of noise exposure in the chinchilla cochlea.", "content": "Chinchillas were exposed to pink noise at levels ranging from 110 dB (for 8 h) to 125 dB (for 1/4 h). After a 3-week survival period, the animals were terminated without anesthesia and tissues were prepared using a soft-surface preparation technique [Axelsson et al., Acta Otolaryngol. 80, 362 (1975)]. Cochlear hair cell damage as well as vascular pathology was slight. On occasion, a vessel or vessel segment was obviously atypical, thus allowing noise-exposed animals to be differentiated from their non-noise-exposed controls. More often, however, differences between animals were more discrete and only evident through a computer analysis of the data. The specific effects of these particular noise-exposure parameters on the cochlear blood supply of the chinchilla will be discussed.", "contents": "Some vascular effects of noise exposure in the chinchilla cochlea. Chinchillas were exposed to pink noise at levels ranging from 110 dB (for 8 h) to 125 dB (for 1/4 h). After a 3-week survival period, the animals were terminated without anesthesia and tissues were prepared using a soft-surface preparation technique [Axelsson et al., Acta Otolaryngol. 80, 362 (1975)]. Cochlear hair cell damage as well as vascular pathology was slight. On occasion, a vessel or vessel segment was obviously atypical, thus allowing noise-exposed animals to be differentiated from their non-noise-exposed controls. More often, however, differences between animals were more discrete and only evident through a computer analysis of the data. The specific effects of these particular noise-exposure parameters on the cochlear blood supply of the chinchilla will be discussed."}
{"id": "PMID:485958", "title": "Assessment of peripheral arterial disease.", "content": "Atherosclerosis is epidemic in our community. Symptomatic peripheral arterial disease affects five to seven per cent of the population by retiring age. The major risk factors are smoking, high blood lipids and diabetes. A clinical history and examination of the peripheral pulses will precisely define the atherosclerotic occlusive lesions. Arteriography is only used as a preoperative measure in those patients requiring reconstructive vascular surgery.", "contents": "Assessment of peripheral arterial disease. Atherosclerosis is epidemic in our community. Symptomatic peripheral arterial disease affects five to seven per cent of the population by retiring age. The major risk factors are smoking, high blood lipids and diabetes. A clinical history and examination of the peripheral pulses will precisely define the atherosclerotic occlusive lesions. Arteriography is only used as a preoperative measure in those patients requiring reconstructive vascular surgery."}
{"id": "PMID:485959", "title": "Techniques of reconstruction for arterial insufficiency.", "content": "Arterial surgery has developed rapidly over the past thirty years, although the principles were well established at the start of the century. This article discusses the techniques currently used for arterial reconstruction of occlusive disease in the aorta, iliac and femoral arteries, and for embolectomy.", "contents": "Techniques of reconstruction for arterial insufficiency. Arterial surgery has developed rapidly over the past thirty years, although the principles were well established at the start of the century. This article discusses the techniques currently used for arterial reconstruction of occlusive disease in the aorta, iliac and femoral arteries, and for embolectomy."}
{"id": "PMID:485960", "title": "The management of intermittent claudication.", "content": "Many patients with intermittent claudication improve due to development of collateral vessels. Only a small proportion worsen, and few progress to severe ischaemia with the risk of amputation. Accordingly, most patients can be reassured and treated expectantly. Those patients have a reduced life expectancy compared with the population at large. Thus, surgical treatment should be reserved for patients who are severely restricted by claudication. The early technical results of arterial reconstruction for claudication are excellent. However, the late results are much worse in patients who continue to smoke. Nowadays, the complication rate from surgery is low.", "contents": "The management of intermittent claudication. Many patients with intermittent claudication improve due to development of collateral vessels. Only a small proportion worsen, and few progress to severe ischaemia with the risk of amputation. Accordingly, most patients can be reassured and treated expectantly. Those patients have a reduced life expectancy compared with the population at large. Thus, surgical treatment should be reserved for patients who are severely restricted by claudication. The early technical results of arterial reconstruction for claudication are excellent. However, the late results are much worse in patients who continue to smoke. Nowadays, the complication rate from surgery is low."}
{"id": "PMID:485961", "title": "Management of the severely ischaemic leg.", "content": "Ischaemic rest pain or tissue necrosis in the foot requires arterial reconstruction or lumbar sympathectomy if major amputation is to be avoided. Arterial reconstruction is preferred but if it is not possible, lumbar sympathectomy frequently is adequate. Conservative treatment is rarely successful. Arterial reconstruction is a relatively safe procedure, even in the elderly. The long-term survival rates are reasonably good, though appreciably less than for the general community. Many amputees have a short life expectancy and do not regain independence.", "contents": "Management of the severely ischaemic leg. Ischaemic rest pain or tissue necrosis in the foot requires arterial reconstruction or lumbar sympathectomy if major amputation is to be avoided. Arterial reconstruction is preferred but if it is not possible, lumbar sympathectomy frequently is adequate. Conservative treatment is rarely successful. Arterial reconstruction is a relatively safe procedure, even in the elderly. The long-term survival rates are reasonably good, though appreciably less than for the general community. Many amputees have a short life expectancy and do not regain independence."}
{"id": "PMID:485962", "title": "Unusual causes of arterial insufficiency.", "content": "Most patients with vascular insufficiency in the legs suffer from atherosclerosis. Thus, it is easy to miss the diagnosis in occasional patients with disease due to other causes. Mechanical and functional disorders which affect major arteries or arterioles are described.", "contents": "Unusual causes of arterial insufficiency. Most patients with vascular insufficiency in the legs suffer from atherosclerosis. Thus, it is easy to miss the diagnosis in occasional patients with disease due to other causes. Mechanical and functional disorders which affect major arteries or arterioles are described."}
{"id": "PMID:485964", "title": "Relationship of smoking to peripheral arterial disease.", "content": "Smoking is the most prevalent 'risk factor' causing atherosclerosis. The association is strongest for disease in arteries to the legs. Continued smoking worsens claudication, increases the risk of progression to gangrene, and decreases long-term patency rates of arterial reconstructions.", "contents": "Relationship of smoking to peripheral arterial disease. Smoking is the most prevalent 'risk factor' causing atherosclerosis. The association is strongest for disease in arteries to the legs. Continued smoking worsens claudication, increases the risk of progression to gangrene, and decreases long-term patency rates of arterial reconstructions."}
{"id": "PMID:485966", "title": "Prevalence of compound analgesic in Newcastle (NSW).", "content": "A household survey of analgesic use was carried out in two areas of different social advantage in Newcastle, New South Wales, during November 1977. The survey was preliminary to evaluating the proposed rescheduling of compound analgesics in the region. Approximately 16.5 per cent of the over 18-year-olds in the population claimed to use compound analgesics from time to time, and over 90 per cent used analgesics of some kind. Analgesic patterns varied between the two areas studied; of women living in the socially disadvantaged areas, about five per cent used compound analgesics daily. Rescheduling of compound analgesics may initially increase the workload of general practitioners, as up to one third of women who regularly use analgesics seek alternatives. A programme of public education and support is needed to assist compound analgesic users when rescheduling occurs.", "contents": "Prevalence of compound analgesic in Newcastle (NSW). A household survey of analgesic use was carried out in two areas of different social advantage in Newcastle, New South Wales, during November 1977. The survey was preliminary to evaluating the proposed rescheduling of compound analgesics in the region. Approximately 16.5 per cent of the over 18-year-olds in the population claimed to use compound analgesics from time to time, and over 90 per cent used analgesics of some kind. Analgesic patterns varied between the two areas studied; of women living in the socially disadvantaged areas, about five per cent used compound analgesics daily. Rescheduling of compound analgesics may initially increase the workload of general practitioners, as up to one third of women who regularly use analgesics seek alternatives. A programme of public education and support is needed to assist compound analgesic users when rescheduling occurs."}
{"id": "PMID:485967", "title": "First aid treatment of burns.", "content": "A burn refers to the damage caused to the skin, and sometimes deeper structures, by heat (flame, scald, contact, electricity), chemical agents or radiation. Methods of first-aid treatment of burns are varied, imprecise and sometimes harmful. This article deals with the major principles involved in first-aid, which when implemented will limit the damage created by the burn. The medical profession must be aware of what can be achieved and the necessary steps in the initial treatment of the burn. The profession is called upon to offer first-aid, to give advice and to lecture on accidents in our community, so it is necessary to have a clear plan of treatment.", "contents": "First aid treatment of burns. A burn refers to the damage caused to the skin, and sometimes deeper structures, by heat (flame, scald, contact, electricity), chemical agents or radiation. Methods of first-aid treatment of burns are varied, imprecise and sometimes harmful. This article deals with the major principles involved in first-aid, which when implemented will limit the damage created by the burn. The medical profession must be aware of what can be achieved and the necessary steps in the initial treatment of the burn. The profession is called upon to offer first-aid, to give advice and to lecture on accidents in our community, so it is necessary to have a clear plan of treatment."}
{"id": "PMID:485968", "title": "The growth of medical manpower in New South Wales.", "content": "The postwar measures taken to increase medical manpower in New South Wales have been most successful, to the extent that the present number of medical practitioners available to the population exceeds the recommended level. These efforts have also been reasonably successful in providing more primary medical care in both country and some metropolitan areas. Calculations have been made to estimate how many doctors will be available to the year 1996 if; (a) present proposed training numbers are continued; (b) medical graduates are reduced by 100 per annum from 1985 (that is, 1980 student intakes); (c) medical graduates are reduced by 200 per annum from 1985. For the purpose of these calculations the immigration of doctors was neglected, which in the past has been responsible for a large percentage of the medical workforce. These conservative estimates show that with the 200 per annum reduction, there would still be an excess of doctors by 1991, but that the doctor/population ratio would be close to the recommended ratio by 1996.", "contents": "The growth of medical manpower in New South Wales. The postwar measures taken to increase medical manpower in New South Wales have been most successful, to the extent that the present number of medical practitioners available to the population exceeds the recommended level. These efforts have also been reasonably successful in providing more primary medical care in both country and some metropolitan areas. Calculations have been made to estimate how many doctors will be available to the year 1996 if; (a) present proposed training numbers are continued; (b) medical graduates are reduced by 100 per annum from 1985 (that is, 1980 student intakes); (c) medical graduates are reduced by 200 per annum from 1985. For the purpose of these calculations the immigration of doctors was neglected, which in the past has been responsible for a large percentage of the medical workforce. These conservative estimates show that with the 200 per annum reduction, there would still be an excess of doctors by 1991, but that the doctor/population ratio would be close to the recommended ratio by 1996."}
{"id": "PMID:485971", "title": "Undergraduate teaching in primary medical care.", "content": "There is considerable variation between medical schools both in the timing of undergraduate teaching of general practice and in the methods of teaching. In 1976, the Faculty of Medicine in the University of Melbourne determined that the period of exposure of students to teaching primary medical care would be two weeks in the fourth year, and an optional eight weeks in the sixth year. This paper describes the fourth year teaching programme and its assessment by the students. Essentially, it consists of a two week course of clinical general practice in which an attempt is made to integrate the knowledge and experience gained from both hospital and community medicine.", "contents": "Undergraduate teaching in primary medical care. There is considerable variation between medical schools both in the timing of undergraduate teaching of general practice and in the methods of teaching. In 1976, the Faculty of Medicine in the University of Melbourne determined that the period of exposure of students to teaching primary medical care would be two weeks in the fourth year, and an optional eight weeks in the sixth year. This paper describes the fourth year teaching programme and its assessment by the students. Essentially, it consists of a two week course of clinical general practice in which an attempt is made to integrate the knowledge and experience gained from both hospital and community medicine."}
{"id": "PMID:485972", "title": "Growth, reproductive productivity and longevity of mice fed polyunsaturated ruminant-derived foodstuffs.", "content": "The effects on the well-being of mice of feeding linoleic acid-enriched foodstuffs derived from ruminants receiving protected polyunsaturated oil were determined. Growth, reproductive productivity and longevity were compared in mice fed freeze-dried human diets containing either these products or the corresponding conventional ruminant-derived foods. A laboratory mouse pellet diet was used as a standard for the comparison. Growth rates and life spans were found to be similar in all three groups. Although the reproductive productivity of the mice on the polyunsaturated diet was never significantly different from that of mice on the conventional human diet, productivities of both groups on human diets were usually significantly below those of mice eating pellets. By the third generation of the second reproduction study, productivities of both these groups of mice had returned to the same level as those of mice eating pellets. Possible reasons for the depression in reproductive productivity in mice on the human diets are discussed. It was concluded that the polyunsaturated human diet neither impaired nor improved the lives of the animals eating the diet relative to the lives of animals eating the conventional human diet.", "contents": "Growth, reproductive productivity and longevity of mice fed polyunsaturated ruminant-derived foodstuffs. The effects on the well-being of mice of feeding linoleic acid-enriched foodstuffs derived from ruminants receiving protected polyunsaturated oil were determined. Growth, reproductive productivity and longevity were compared in mice fed freeze-dried human diets containing either these products or the corresponding conventional ruminant-derived foods. A laboratory mouse pellet diet was used as a standard for the comparison. Growth rates and life spans were found to be similar in all three groups. Although the reproductive productivity of the mice on the polyunsaturated diet was never significantly different from that of mice on the conventional human diet, productivities of both groups on human diets were usually significantly below those of mice eating pellets. By the third generation of the second reproduction study, productivities of both these groups of mice had returned to the same level as those of mice eating pellets. Possible reasons for the depression in reproductive productivity in mice on the human diets are discussed. It was concluded that the polyunsaturated human diet neither impaired nor improved the lives of the animals eating the diet relative to the lives of animals eating the conventional human diet."}
{"id": "PMID:485974", "title": "Metabolism and defluorination of fluoroacetate in the brush-tailed possum (Trichosurus vulpecula).", "content": "The brush-tailed possum (T. vulpecula) from Western Australia was found to be nearly 150 times more resistant to fluoroacetate intoxication in vivo than the same species from South Australia. Acetone powder preparations from the liver of animals from both populations showed similar abilities to convert fluoroacetate into fluorocitrate. Aconitate hydratase activity in liver preparations from both Western Australian and South Australian animals was similarly and competitively inhibited by fluorocitrate. Both animals were capable of defluorinating fluoroacetate at similar rates by a glutathione-dependent enzymic mechanism resulting in the formation of free fluoride ion and S-carboxymethylcysteine. Glutathione was also capable of partial protection against the toxic effects of fluoroacetate in vitro by a further unelucidated mechanism.", "contents": "Metabolism and defluorination of fluoroacetate in the brush-tailed possum (Trichosurus vulpecula). The brush-tailed possum (T. vulpecula) from Western Australia was found to be nearly 150 times more resistant to fluoroacetate intoxication in vivo than the same species from South Australia. Acetone powder preparations from the liver of animals from both populations showed similar abilities to convert fluoroacetate into fluorocitrate. Aconitate hydratase activity in liver preparations from both Western Australian and South Australian animals was similarly and competitively inhibited by fluorocitrate. Both animals were capable of defluorinating fluoroacetate at similar rates by a glutathione-dependent enzymic mechanism resulting in the formation of free fluoride ion and S-carboxymethylcysteine. Glutathione was also capable of partial protection against the toxic effects of fluoroacetate in vitro by a further unelucidated mechanism."}
{"id": "PMID:485975", "title": "Changes in body composition and efficiency of food utilization for growth in young adult female rats before, during and after a period of food restriction.", "content": "Changes in body composition were studied in three groups of young adult female rats; the treatments were (1) ad libitum food intake to obtain normal growth, (2) restricted food intake to cause body weight loss, and (3) restricted followed by ad libitum food intake to obtain recovery of lost body weight. In each of the three groups of rats the percentage body water was linearly and negatively correlated with the percentage body fat, the weight of body water was linearly and positively correlated with the weight of body protein, and the ratio of the weight of body protein to water was relatively constant at 1:3.20 +/- 0.02 (mean +/- standard error). The percentage body water in the fat-free body was linearly and negatively correlated with fat-free body weight during normal growth between 109 and 334 g body weight but positively correlated during body weight loss and recovery. During recovery of body weight rats laid down more fat and less protein than during normal growth through the same body weight range and the percentage of digestible energy retained as body tissue was increased.", "contents": "Changes in body composition and efficiency of food utilization for growth in young adult female rats before, during and after a period of food restriction. Changes in body composition were studied in three groups of young adult female rats; the treatments were (1) ad libitum food intake to obtain normal growth, (2) restricted food intake to cause body weight loss, and (3) restricted followed by ad libitum food intake to obtain recovery of lost body weight. In each of the three groups of rats the percentage body water was linearly and negatively correlated with the percentage body fat, the weight of body water was linearly and positively correlated with the weight of body protein, and the ratio of the weight of body protein to water was relatively constant at 1:3.20 +/- 0.02 (mean +/- standard error). The percentage body water in the fat-free body was linearly and negatively correlated with fat-free body weight during normal growth between 109 and 334 g body weight but positively correlated during body weight loss and recovery. During recovery of body weight rats laid down more fat and less protein than during normal growth through the same body weight range and the percentage of digestible energy retained as body tissue was increased."}
{"id": "PMID:485976", "title": "Sulfur and methionine metabolism in sheep. I. First approximations of sulfur pools in and sulfur flows from the reticulo-rumen.", "content": "Sulfur pools in the rumen and sulfur flows from the rumen were investigated in two experiments with sheep on a diet containing equal parts of oaten and lucerne chaffs. The diet was fed at two levels, either chopped or pelleted, and with intraruminal DL-methionine supplements. Ruminal fluid volumes and fluid flows to the omasum were measured. None of the treatments influenced ruminal fluid volume. Fluid flow to the omasum, however, was increased by increasing dry matter intake (DMI), and was further enhanced by feeding chaffed hay rather than the same materials ground and pelleted; the DL-methionine supplement had no effect. First approximation of the ruminal sulfur pools and of sulfur flows to the omasum were derived from the concentration of sulfur in true digesta and the ruminal fluid volume or fluid flow. Increasing DMI from 500 to 1000 g/day resulted in larger ruminal pools of total (1096 v. 792 mg), neutral (1016 v. 731 mg) and protein (479 v. 419 mg) sulfur, but the reducible sulfur pools were not affected by the level of DMI. Infusions of DL-methionine increased the ruminal sulfide sulfur pool irrespective of level of DMI. The first approximation of total sulfur flow was increased by 1660 mg/day at the higher level of DMI, due mainly to increases of 710 mg S/day as protein sulfur and 859 mg S/day as non-protein neutral sulfur. Flows of inorganic sulfate and ester sulfate sulfur, although small in comparison with organic sulfur flows, increased with level of DMI. Sulfide sulfur flows were also increased at the higher level of DMI, and were almost doubled by intraruminal infusions of DL-methionine.", "contents": "Sulfur and methionine metabolism in sheep. I. First approximations of sulfur pools in and sulfur flows from the reticulo-rumen. Sulfur pools in the rumen and sulfur flows from the rumen were investigated in two experiments with sheep on a diet containing equal parts of oaten and lucerne chaffs. The diet was fed at two levels, either chopped or pelleted, and with intraruminal DL-methionine supplements. Ruminal fluid volumes and fluid flows to the omasum were measured. None of the treatments influenced ruminal fluid volume. Fluid flow to the omasum, however, was increased by increasing dry matter intake (DMI), and was further enhanced by feeding chaffed hay rather than the same materials ground and pelleted; the DL-methionine supplement had no effect. First approximation of the ruminal sulfur pools and of sulfur flows to the omasum were derived from the concentration of sulfur in true digesta and the ruminal fluid volume or fluid flow. Increasing DMI from 500 to 1000 g/day resulted in larger ruminal pools of total (1096 v. 792 mg), neutral (1016 v. 731 mg) and protein (479 v. 419 mg) sulfur, but the reducible sulfur pools were not affected by the level of DMI. Infusions of DL-methionine increased the ruminal sulfide sulfur pool irrespective of level of DMI. The first approximation of total sulfur flow was increased by 1660 mg/day at the higher level of DMI, due mainly to increases of 710 mg S/day as protein sulfur and 859 mg S/day as non-protein neutral sulfur. Flows of inorganic sulfate and ester sulfate sulfur, although small in comparison with organic sulfur flows, increased with level of DMI. Sulfide sulfur flows were also increased at the higher level of DMI, and were almost doubled by intraruminal infusions of DL-methionine."}
{"id": "PMID:485977", "title": "Sulfur and methionine metabolism in sheep. II. Quantitative estimates of sulfur metabolism in the sheep's stomach.", "content": "The metabolism of dietary and supplemental DL-methionine sulfur in the stomach of sheep was studied in two experiments. In both experiments sheep were fed a 50 : 50 oaten chaff: lucerne chaff ration at two levels of intake, and some animals received intraruminal infusions of DL-methionine. In experiment 2 increasing dry matter intake (DMI) increased first approximations of total, neutral, protein and reducible sulfur flows and also sulfide sulfur flow from the reticulo-rumen. Increased DMI (from 500 to 1000 g/day) also resulted in greater true flows of total (2207 v. 1104 mg/day), neutral (1867 v. 1043 mg/day) and protein (893 v. 482 mg/day) sulfur at the duodenum. Two flow diagrams of sulfur metabolism in the compartments of the ruminant stomach were developed from the data of experiment 2. Fluid flows of sulfur in experiment 1 were used to supplement the data of experiment 2 in developing the balance models. The two models represent the extremes of dietary and supplemental sulfur metabolism in the sheep's stomach under the conditions of experiment 2, and they are discussed in relation to previous research on sulfur metabolism in the stomach.", "contents": "Sulfur and methionine metabolism in sheep. II. Quantitative estimates of sulfur metabolism in the sheep's stomach. The metabolism of dietary and supplemental DL-methionine sulfur in the stomach of sheep was studied in two experiments. In both experiments sheep were fed a 50 : 50 oaten chaff: lucerne chaff ration at two levels of intake, and some animals received intraruminal infusions of DL-methionine. In experiment 2 increasing dry matter intake (DMI) increased first approximations of total, neutral, protein and reducible sulfur flows and also sulfide sulfur flow from the reticulo-rumen. Increased DMI (from 500 to 1000 g/day) also resulted in greater true flows of total (2207 v. 1104 mg/day), neutral (1867 v. 1043 mg/day) and protein (893 v. 482 mg/day) sulfur at the duodenum. Two flow diagrams of sulfur metabolism in the compartments of the ruminant stomach were developed from the data of experiment 2. Fluid flows of sulfur in experiment 1 were used to supplement the data of experiment 2 in developing the balance models. The two models represent the extremes of dietary and supplemental sulfur metabolism in the sheep's stomach under the conditions of experiment 2, and they are discussed in relation to previous research on sulfur metabolism in the stomach."}
{"id": "PMID:485978", "title": "Ovarian response to exogenous hormones in six-week-old lambs.", "content": "Crossbred lambs 5--6 weeks old were treated with human chorionic gonadotrophin (hCG) (500 or 1500 i.u.) alone, hCG plus pregnant mare serum gonadotrophin (PMSG) (1000 or 2000 i.u.), 1000 i.u. PMSG alone, or were untreated. PMSG alone and PMSG + hCG increased ovarian weight and uterine weight. PMSG alone stimulated growth and luteinization of follicles but PMSG + hCG induced ovulations and formation of corpora lutea. hCG alone did not change any of the characteristics which were measured. PMSG had a significant effect on the number of vesicular follicles but none of the treatments affected the number of growing follicles.", "contents": "Ovarian response to exogenous hormones in six-week-old lambs. Crossbred lambs 5--6 weeks old were treated with human chorionic gonadotrophin (hCG) (500 or 1500 i.u.) alone, hCG plus pregnant mare serum gonadotrophin (PMSG) (1000 or 2000 i.u.), 1000 i.u. PMSG alone, or were untreated. PMSG alone and PMSG + hCG increased ovarian weight and uterine weight. PMSG alone stimulated growth and luteinization of follicles but PMSG + hCG induced ovulations and formation of corpora lutea. hCG alone did not change any of the characteristics which were measured. PMSG had a significant effect on the number of vesicular follicles but none of the treatments affected the number of growing follicles."}
{"id": "PMID:485979", "title": "Effect of treatment with azathioprine on the responses of rat lymphocytes to phytohaemagglutinin.", "content": "The proliferative responses of rat peripheral blood lymphocytes (PBL) and spleen cells to phytohaemagglutinin (PHA) were studied after single or multiple (daily for 4 days) injections of azathioprine (AZ). Lymphopenia developed within 4 h of a single dose (78 mg/kg) of AZ and persisted for at least 72 h. There was no lymphopenia 24 h after the last of 4 daily injections. In vitro, PBL were more sensitive than spleen cells to the inhibitory effect of AZ. Likewise, the responses of PBL were relatively more depressed than those of spleen cells after single or multiple injections of AZ. The degree of depression was less than was expected from the effect of AZ in vitro. Multiple small doses were more depressive than multiple large doses. Serum from treated rats, used at 20% concentration, was more depressive than normal. Thus, rat lymphocytes are quite sensitive to AZ in vitro, but appear to be relatively resistant in vivo, this resistance resembling the resistance of the primary antibody response to AZ treatment.", "contents": "Effect of treatment with azathioprine on the responses of rat lymphocytes to phytohaemagglutinin. The proliferative responses of rat peripheral blood lymphocytes (PBL) and spleen cells to phytohaemagglutinin (PHA) were studied after single or multiple (daily for 4 days) injections of azathioprine (AZ). Lymphopenia developed within 4 h of a single dose (78 mg/kg) of AZ and persisted for at least 72 h. There was no lymphopenia 24 h after the last of 4 daily injections. In vitro, PBL were more sensitive than spleen cells to the inhibitory effect of AZ. Likewise, the responses of PBL were relatively more depressed than those of spleen cells after single or multiple injections of AZ. The degree of depression was less than was expected from the effect of AZ in vitro. Multiple small doses were more depressive than multiple large doses. Serum from treated rats, used at 20% concentration, was more depressive than normal. Thus, rat lymphocytes are quite sensitive to AZ in vitro, but appear to be relatively resistant in vivo, this resistance resembling the resistance of the primary antibody response to AZ treatment."}
{"id": "PMID:485980", "title": "Serological responses of sheep and cattle exposed to natural Toxoplasma infection.", "content": "During a four and a half year period a group of cattle was grazed continuously with a group of sheep and observed for Toxoplasma antibodies. Initially, there were 25 cattle and 31 sheep, but these were reduced to 19 and 18, respectively, by the end of the observation. Only transient antibody responses were noted in 2 cattle, whereas persistent titres developed in 12 sheep. Interpreted in the light of available information, these results were taken to indicate that cattle do not readily acquire persistent T. gondii infections.", "contents": "Serological responses of sheep and cattle exposed to natural Toxoplasma infection. During a four and a half year period a group of cattle was grazed continuously with a group of sheep and observed for Toxoplasma antibodies. Initially, there were 25 cattle and 31 sheep, but these were reduced to 19 and 18, respectively, by the end of the observation. Only transient antibody responses were noted in 2 cattle, whereas persistent titres developed in 12 sheep. Interpreted in the light of available information, these results were taken to indicate that cattle do not readily acquire persistent T. gondii infections."}
{"id": "PMID:485981", "title": "The kinetic behaviour of radiolabelled IgG1 derived from different sources in adult sheep and neonatal lambs.", "content": "The turnover rate and pool volume of radiolabelled autologous IgG1 was similar to homologous IgG1 in adult sheep. In neonatal lambs there was no significant difference for either of these parameters between homologous serum IgG1 and maternal colostrum IgG1. However, the pool volume for IgG1 expanded by approx. 30% over the experimental period in the lambs. When the turnover rates were corrected for this increase they were found to be similar to those observed for IgG1 in adult animals.", "contents": "The kinetic behaviour of radiolabelled IgG1 derived from different sources in adult sheep and neonatal lambs. The turnover rate and pool volume of radiolabelled autologous IgG1 was similar to homologous IgG1 in adult sheep. In neonatal lambs there was no significant difference for either of these parameters between homologous serum IgG1 and maternal colostrum IgG1. However, the pool volume for IgG1 expanded by approx. 30% over the experimental period in the lambs. When the turnover rates were corrected for this increase they were found to be similar to those observed for IgG1 in adult animals."}
{"id": "PMID:485982", "title": "Cytotoxicity studies of human melanoma cells and fibroblasts.", "content": "In seven human melanoma cell lines and one human fibroblast strain some correlation of resistance to cell killing was found with two bifunctional alkylating agents (melphalan, chlorambucil) and three monofunctional agents (4(5)-(3,3-dimethyl-l-triazeno)imidazole-5(4)-carboxamide (DTIC), methylmethane sulphonate (MMS) and N-methyl-N1-nitro-N-nitrosoguanidine (MNNG), but little cross-resistance was found between these two groups of agents or with cytosine arabinoside (ara-C). In contrast to previous studies with rodent tumours, potentially synergistic (chloroquine, arginine) or antagonistic (ascorbic acid, leucine) compounds did not affect the toxicity of melphalan in a human melanoma cell line. In two melanoma lines DTIC induced patterns of DNA damage (inhibition of semi-conservative synthesis) and repair (strand breaks and repair synthesis) similar to, but not identical with, those induced by the methylating agent MMNG. These results suggest that a methylating species is derived from DTIC but has a different reactivity toward DNA compared with MNNG.", "contents": "Cytotoxicity studies of human melanoma cells and fibroblasts. In seven human melanoma cell lines and one human fibroblast strain some correlation of resistance to cell killing was found with two bifunctional alkylating agents (melphalan, chlorambucil) and three monofunctional agents (4(5)-(3,3-dimethyl-l-triazeno)imidazole-5(4)-carboxamide (DTIC), methylmethane sulphonate (MMS) and N-methyl-N1-nitro-N-nitrosoguanidine (MNNG), but little cross-resistance was found between these two groups of agents or with cytosine arabinoside (ara-C). In contrast to previous studies with rodent tumours, potentially synergistic (chloroquine, arginine) or antagonistic (ascorbic acid, leucine) compounds did not affect the toxicity of melphalan in a human melanoma cell line. In two melanoma lines DTIC induced patterns of DNA damage (inhibition of semi-conservative synthesis) and repair (strand breaks and repair synthesis) similar to, but not identical with, those induced by the methylating agent MMNG. These results suggest that a methylating species is derived from DTIC but has a different reactivity toward DNA compared with MNNG."}
{"id": "PMID:485983", "title": "Oxalate excretion in rats injected with xylitol or glycollate: stimulation by phenobarbitone pre-treatment.", "content": "The hypothesis that the prior intake of barbiturates may predispose patients to form increased amounts of oxalate following the intravenous infusion of xylitol was investigated in the rat. Phenobarbitone pre-treatment resulted in a 2-3 fold increase in urinary  [14C] oxalate concentration following the intraperitoneal injection of [U-14C] xylitol or [l -14C] glycollate. The absence of any marked changes in urine volumes and creatinine excretion implied that this increase in urinary oxalate excretion was due to the enhanced synthesis of oxalate. The activities of key enzymes in hepatic oxalate synthesis, glycollate oxidase, lactate dehydrogenase, catalase and alanine aminotransferase were not altered by phenobarbitone pre-treatment. It is suggested that the increased activity of the microsomal mixed function oxidases, following phenobarbitone treatment, may facilitate the oxidation of glycollate and possibly xylitol. This communication leads experimental support to the concept that the prior intake of drugs, such as barbiturates, may predispose patients to form increased amounts of oxalate.", "contents": "Oxalate excretion in rats injected with xylitol or glycollate: stimulation by phenobarbitone pre-treatment. The hypothesis that the prior intake of barbiturates may predispose patients to form increased amounts of oxalate following the intravenous infusion of xylitol was investigated in the rat. Phenobarbitone pre-treatment resulted in a 2-3 fold increase in urinary  [14C] oxalate concentration following the intraperitoneal injection of [U-14C] xylitol or [l -14C] glycollate. The absence of any marked changes in urine volumes and creatinine excretion implied that this increase in urinary oxalate excretion was due to the enhanced synthesis of oxalate. The activities of key enzymes in hepatic oxalate synthesis, glycollate oxidase, lactate dehydrogenase, catalase and alanine aminotransferase were not altered by phenobarbitone pre-treatment. It is suggested that the increased activity of the microsomal mixed function oxidases, following phenobarbitone treatment, may facilitate the oxidation of glycollate and possibly xylitol. This communication leads experimental support to the concept that the prior intake of drugs, such as barbiturates, may predispose patients to form increased amounts of oxalate."}
{"id": "PMID:485984", "title": "A serological survey of Australian wildlife for antibodies to Leptospires of the Hebdomadis serogroup.", "content": "A serological survey for antibodies to Leptospira interrograns serovar hardjo was conducted on 574 serum samples from 10 native and 4 introduced wildlife species in south-eastern Australia. The microscopic agglutination (MA) test was used, and titres to hardjo antigen were detected in 33.5% of 352 brushtailed possums (Trichosurus vulpecula) sampled in several areas of Victoria. Prevalence of reactors ranged from 14 to 66% in 4 populations examined intensively. Serovar balcanica was isolated from possums with hardjo antibodies from two different areas. Of 20 wombats Vombatus ursinus) examined in Victoria, antibodies to hardjo were found in sera from 4 and titres to Pyrogenes and Pomona serogroups were detected in another. Hardjo antibodies were demonstrated in sera from 13 of 19 rusa deer (Cervus timorensis). Negative MA test results to hardjo antigens were recorded in 55 mountain possums (T. caninus), 63 macropods (Macropus spp.), 17 water rats (Hydrmys chrysogaster), 39 fallow deer (Dama dama), 2 hog deer (Axis porcinus) and 2 water buffalo (Bubalus bubalus). No MA antibodies to any of 16 leptospiral serogroups were detected in 17 water rats tested. Kidneys were examined from 330 of these animals and focal interstitial nephritis suggestive of leptospirosis was found in kidneys of 63 of 169 T. vulpecula, 3 of 55 T. caninus, 12 of 18 V. ursinus, 6 of 22 Macropus spp., 9 of 16 H. chrysogaster, 5 of 11 C. timorensis and 3 of 39 D. dama. A statistical association between focal interstitial nephritis and MA antibodies to hardjo was found in T. vulpecula.", "contents": "A serological survey of Australian wildlife for antibodies to Leptospires of the Hebdomadis serogroup. A serological survey for antibodies to Leptospira interrograns serovar hardjo was conducted on 574 serum samples from 10 native and 4 introduced wildlife species in south-eastern Australia. The microscopic agglutination (MA) test was used, and titres to hardjo antigen were detected in 33.5% of 352 brushtailed possums (Trichosurus vulpecula) sampled in several areas of Victoria. Prevalence of reactors ranged from 14 to 66% in 4 populations examined intensively. Serovar balcanica was isolated from possums with hardjo antibodies from two different areas. Of 20 wombats Vombatus ursinus) examined in Victoria, antibodies to hardjo were found in sera from 4 and titres to Pyrogenes and Pomona serogroups were detected in another. Hardjo antibodies were demonstrated in sera from 13 of 19 rusa deer (Cervus timorensis). Negative MA test results to hardjo antigens were recorded in 55 mountain possums (T. caninus), 63 macropods (Macropus spp.), 17 water rats (Hydrmys chrysogaster), 39 fallow deer (Dama dama), 2 hog deer (Axis porcinus) and 2 water buffalo (Bubalus bubalus). No MA antibodies to any of 16 leptospiral serogroups were detected in 17 water rats tested. Kidneys were examined from 330 of these animals and focal interstitial nephritis suggestive of leptospirosis was found in kidneys of 63 of 169 T. vulpecula, 3 of 55 T. caninus, 12 of 18 V. ursinus, 6 of 22 Macropus spp., 9 of 16 H. chrysogaster, 5 of 11 C. timorensis and 3 of 39 D. dama. A statistical association between focal interstitial nephritis and MA antibodies to hardjo was found in T. vulpecula."}
{"id": "PMID:485985", "title": "A sero-epidemiological study of Leptospira interrogans serovar Balcanica in four brush-tailed possum populations in Victoria, Australia.", "content": "The microscopic agglutination (MA) test was utilised to study the prevalence of antibodies to Leptospira interrogans serovar hardjo in 4 populations of brush-tailed possums (Trichosurus vulpecula). The overall antibody prevalence varied from 14% to 66%; however, the age distribution of MA test titres was remarkably similar in all 4 populations. Antibody prevalence was similar in both males and females and demonstrable antibodies were limited to sexually mature animals. The greater prevalence of high titres (greater than or equal to 1:128) in the 18- to 24-month age group suggested that primary infections were acquired at this age. The findings suggested that infection was maintained in possum populations by direct transmission, probably associated with breeding. Focal interstitial nephritis was observed in kidneys of possums greater than 18 months of age and was associated with MA titres to hardjo (P less than 0.001). Serovar balcanica was isolated from possum kidneys from 2 of these populations, suggesting that balcanica infections were responsible for most of the hardjo titres. However, agglutinin-absorption tests indicated that some possums may be infected with a leptospire more closely related to hardjo than to balcanica.", "contents": "A sero-epidemiological study of Leptospira interrogans serovar Balcanica in four brush-tailed possum populations in Victoria, Australia. The microscopic agglutination (MA) test was utilised to study the prevalence of antibodies to Leptospira interrogans serovar hardjo in 4 populations of brush-tailed possums (Trichosurus vulpecula). The overall antibody prevalence varied from 14% to 66%; however, the age distribution of MA test titres was remarkably similar in all 4 populations. Antibody prevalence was similar in both males and females and demonstrable antibodies were limited to sexually mature animals. The greater prevalence of high titres (greater than or equal to 1:128) in the 18- to 24-month age group suggested that primary infections were acquired at this age. The findings suggested that infection was maintained in possum populations by direct transmission, probably associated with breeding. Focal interstitial nephritis was observed in kidneys of possums greater than 18 months of age and was associated with MA titres to hardjo (P less than 0.001). Serovar balcanica was isolated from possum kidneys from 2 of these populations, suggesting that balcanica infections were responsible for most of the hardjo titres. However, agglutinin-absorption tests indicated that some possums may be infected with a leptospire more closely related to hardjo than to balcanica."}
{"id": "PMID:485986", "title": "Alteration in cell division, morphology and motility induced by the toxic principle of lupinosis.", "content": "A number of experiments are described in which the effect of the toxins causing lupinosis on cell morphology and motility is examined. The toxins arrested cells in metaphase leading to abnormal profiles, and inhibited leucocyte migration. Similar results were obtained with colchicine on cell division and, from the evidence obtained, it is suggested that the mechanism of action of the two agents is similar and that the same binding sites are probably involved.", "contents": "Alteration in cell division, morphology and motility induced by the toxic principle of lupinosis. A number of experiments are described in which the effect of the toxins causing lupinosis on cell morphology and motility is examined. The toxins arrested cells in metaphase leading to abnormal profiles, and inhibited leucocyte migration. Similar results were obtained with colchicine on cell division and, from the evidence obtained, it is suggested that the mechanism of action of the two agents is similar and that the same binding sites are probably involved."}
{"id": "PMID:485987", "title": "Inhibition of Tamm-Horsfall glycoprotein induction of alkaline phosphatase in cystic fibrosis fibroblasts by medium conditioned by normal cells.", "content": "It is confirmed that the level of alkaline phosphatase in fibroblasts derived from cystic fibrosis patients can be induced many-fold by growing the cells in the presence of Tamm-Horsfall glycoprotein. It is further shown that normal fibroblasts produce a \"CF corrective factor\" which markedly inhibits this phenomenon. These observations support a previous hypothesis on the nature of the metabolic defect in cystic fibrosis.", "contents": "Inhibition of Tamm-Horsfall glycoprotein induction of alkaline phosphatase in cystic fibrosis fibroblasts by medium conditioned by normal cells. It is confirmed that the level of alkaline phosphatase in fibroblasts derived from cystic fibrosis patients can be induced many-fold by growing the cells in the presence of Tamm-Horsfall glycoprotein. It is further shown that normal fibroblasts produce a \"CF corrective factor\" which markedly inhibits this phenomenon. These observations support a previous hypothesis on the nature of the metabolic defect in cystic fibrosis."}
{"id": "PMID:485999", "title": "Isolation and growth characteristics of psittacine viruses in chicken embryos.", "content": "Eight viruses were isolated in embryonating eggs, from psittacine birds comprising a cockatiel, a budgerigar, 3 parrots, a love bird, and 2 rosellas. Initial attempts at isolation used 3 routes of embryo inoculation: yolk sac, allantoic sac, and chorioallantoic membrane (CAM). The most sensitive route was determined for 6 of the isolates by making comparative titrations by yolk sac, allantoic sac, and CAM routes of inoculation. In growth-curve studies of 6 of the isolates, virus concentration was determined daily for 5-6 days postinoculation in the CAM, allantoamnionic fluid, and liver. Peak virus concentrations appeared in about 72 hours. The embryonic lesions observed are described. Only one of the isolates hemagglutinated fowl red blood cells.", "contents": "Isolation and growth characteristics of psittacine viruses in chicken embryos. Eight viruses were isolated in embryonating eggs, from psittacine birds comprising a cockatiel, a budgerigar, 3 parrots, a love bird, and 2 rosellas. Initial attempts at isolation used 3 routes of embryo inoculation: yolk sac, allantoic sac, and chorioallantoic membrane (CAM). The most sensitive route was determined for 6 of the isolates by making comparative titrations by yolk sac, allantoic sac, and CAM routes of inoculation. In growth-curve studies of 6 of the isolates, virus concentration was determined daily for 5-6 days postinoculation in the CAM, allantoamnionic fluid, and liver. Peak virus concentrations appeared in about 72 hours. The embryonic lesions observed are described. Only one of the isolates hemagglutinated fowl red blood cells."}
{"id": "PMID:486000", "title": "Characterization of paramyxo-, herpes-, and orbiviruses isolated from psittacine birds.", "content": "Isolates of paramyxo-, herpes-, and orbiviruses from psittacine birds were characterized in the course of studies in cell cultures. The LBD-1 isolate, from a lovebird, was grown in chick kidney (CK) cells. It had the properties of a paramyxovirus but was found tobe serologically distinct from known avian paramyxoviruses. This virus was pathogenic for Japanese quail but not for young chickens or budgerigars. RSL-1 and -2, isolated from diseased rosellas, were propagated in chicken embryo fibroblasts and CK cells, and were found similar to other herpesviruses in physicochemical and morphological properties. They were immunologically related to the psittacine herpesvirus which causes Pacheco's parrot disease, and when inoculated into budgerigars produced clinical disease and death with hepatic lesions. The CKT-1 and PKT-1 isolates, from a cockatiel and a budgerigar, grew in fibroblastic cell cultures from several species. Their physicochemical properties suggested that they should be classified as members of the orbivirus group. They were mildly pathogenic for budgerigars.", "contents": "Characterization of paramyxo-, herpes-, and orbiviruses isolated from psittacine birds. Isolates of paramyxo-, herpes-, and orbiviruses from psittacine birds were characterized in the course of studies in cell cultures. The LBD-1 isolate, from a lovebird, was grown in chick kidney (CK) cells. It had the properties of a paramyxovirus but was found tobe serologically distinct from known avian paramyxoviruses. This virus was pathogenic for Japanese quail but not for young chickens or budgerigars. RSL-1 and -2, isolated from diseased rosellas, were propagated in chicken embryo fibroblasts and CK cells, and were found similar to other herpesviruses in physicochemical and morphological properties. They were immunologically related to the psittacine herpesvirus which causes Pacheco's parrot disease, and when inoculated into budgerigars produced clinical disease and death with hepatic lesions. The CKT-1 and PKT-1 isolates, from a cockatiel and a budgerigar, grew in fibroblastic cell cultures from several species. Their physicochemical properties suggested that they should be classified as members of the orbivirus group. They were mildly pathogenic for budgerigars."}
{"id": "PMID:486001", "title": "Toxicity of endotoxin to chicks.", "content": "Large doses of purified lipopolysaccharide (LPS) purified from Escherichia coli induced clinical signs but no mortality in chicks. Five chicks survived a mean dose of 517 mg/kg. One individual that received LPS at 577 mg/kg recovered from clinical manifestations within two days. Attempts failed to produce a generalized Shwartzman-like reaction with two intravenous inoculations of LPS at about 24-hour intervals. Prior injection of uric acid did not protect chicks from LPS by intravenous exposure.", "contents": "Toxicity of endotoxin to chicks. Large doses of purified lipopolysaccharide (LPS) purified from Escherichia coli induced clinical signs but no mortality in chicks. Five chicks survived a mean dose of 517 mg/kg. One individual that received LPS at 577 mg/kg recovered from clinical manifestations within two days. Attempts failed to produce a generalized Shwartzman-like reaction with two intravenous inoculations of LPS at about 24-hour intervals. Prior injection of uric acid did not protect chicks from LPS by intravenous exposure."}
{"id": "PMID:486002", "title": "Isolation of an etiologic agent of acute respiratory disease (rhinotracheitis) of turkey poults.", "content": "A small gram-negative motile bacillus was isolated from laboratory poults affected by acute respiratory disease (rhinotracheitis) of turkeys. The bacterium was inoculated intranasally into susceptible day-old poults; the poults developed typical clinical signs of acute respiratory disease, and the bacterium was reisolated. This same bacterium was isolated from commercial poults with typical signs of acute respiratory disease but not from poults of similar age which were clinically normal. The bacterium has not been identified taxonomically. We conclude that it is a primary etiologic agent for acute respiratory disease of turkey poults.", "contents": "Isolation of an etiologic agent of acute respiratory disease (rhinotracheitis) of turkey poults. A small gram-negative motile bacillus was isolated from laboratory poults affected by acute respiratory disease (rhinotracheitis) of turkeys. The bacterium was inoculated intranasally into susceptible day-old poults; the poults developed typical clinical signs of acute respiratory disease, and the bacterium was reisolated. This same bacterium was isolated from commercial poults with typical signs of acute respiratory disease but not from poults of similar age which were clinically normal. The bacterium has not been identified taxonomically. We conclude that it is a primary etiologic agent for acute respiratory disease of turkey poults."}
{"id": "PMID:486003", "title": "Fungus species isolated from commercial hatcheries in Alabama.", "content": "Selected locations (incubators, incubator rooms, hatchers, hatcher rooms, chick pools, and egg rooms) in six commercial broiler hatcheries in Alabama were sampled for fungus species over a 12-month period (May 1977-April 1978) by open-plate techniques (cornmeal agar or Sabouraud's dextrose agar). Colonies at each location were counted at 24, 48, and 72-120 hours postexposure. Thirty-five species representing 21 genera were identified. The most prevalent genera were Cladosporium, Penicillium, Aspergillus, Geotrichum, and Fusarium. Aspergillus fumigatus was associated with clinical disease in the baby chick on several occasions, and Cladosporium cladosporioides was the most prevalent species isolated. In general, isolations increased in early spring and summer.", "contents": "Fungus species isolated from commercial hatcheries in Alabama. Selected locations (incubators, incubator rooms, hatchers, hatcher rooms, chick pools, and egg rooms) in six commercial broiler hatcheries in Alabama were sampled for fungus species over a 12-month period (May 1977-April 1978) by open-plate techniques (cornmeal agar or Sabouraud's dextrose agar). Colonies at each location were counted at 24, 48, and 72-120 hours postexposure. Thirty-five species representing 21 genera were identified. The most prevalent genera were Cladosporium, Penicillium, Aspergillus, Geotrichum, and Fusarium. Aspergillus fumigatus was associated with clinical disease in the baby chick on several occasions, and Cladosporium cladosporioides was the most prevalent species isolated. In general, isolations increased in early spring and summer."}
{"id": "PMID:486004", "title": "Hemolytic complement in specific-pathogen-free chickens: influence of age and sex.", "content": "Six groups of specific-pathogen-free (SPF) chickens of various ages were tested for hemolytic complement (C) activity with a radial hemolytic diffusion technique. The chickens tested were 2, 6, 7, and 8 weeks old. Chickens 2 weeks old were found to have little hemolytic activity compared with chickens 6 to 8 weeks old. Mean titers were similar in the older chickens. No significant differences were found in C titers between males and females, unlike reports for mice.", "contents": "Hemolytic complement in specific-pathogen-free chickens: influence of age and sex. Six groups of specific-pathogen-free (SPF) chickens of various ages were tested for hemolytic complement (C) activity with a radial hemolytic diffusion technique. The chickens tested were 2, 6, 7, and 8 weeks old. Chickens 2 weeks old were found to have little hemolytic activity compared with chickens 6 to 8 weeks old. Mean titers were similar in the older chickens. No significant differences were found in C titers between males and females, unlike reports for mice."}
{"id": "PMID:486006", "title": "Vaccination of ring-necked pheasant for marble spleen disease.", "content": "Laboratory evidence indicates that a safe and effective procedure was developed for vaccinating pheasants against marble spleen disease. Field trials confirm the safety of the vaccine and suggest that vaccination will prevent marble spleen disease. Vaccination is by drinking-water administration of turkey-spleen-propagated turkey-origin live avirulent virus to pheasants 4 1/2 weeks old or older. The effect of field vaccination was studied in 39,000 pheasants in pens where recurrent annual mortality had been 5-15%.", "contents": "Vaccination of ring-necked pheasant for marble spleen disease. Laboratory evidence indicates that a safe and effective procedure was developed for vaccinating pheasants against marble spleen disease. Field trials confirm the safety of the vaccine and suggest that vaccination will prevent marble spleen disease. Vaccination is by drinking-water administration of turkey-spleen-propagated turkey-origin live avirulent virus to pheasants 4 1/2 weeks old or older. The effect of field vaccination was studied in 39,000 pheasants in pens where recurrent annual mortality had been 5-15%."}
{"id": "PMID:486011", "title": "Response of women mountaineers to maximal exercise during hypoxia.", "content": "Eight members of the American Women's Himalayan Expedition, ranging in age from 20-49, performed maximal exercise on a treadmill under normoxic and acute hypoxic (12.58% O2) conditions. Normoxic values for VO2 max were above average for all subjects and did not decline with age. The mean decrease in VO2 max (26.7%) during hypoxia was equivalent to that reported for younger males, which suggests that age was not a factor in response to hypoxia. Maximal heart rate, respiratory exchange ratio, oxygen pulse, and walk time were lower in hypoxia while ventilatory equivalent and blood lactate were higher. VEmax BTPS, was the same under both conditions. The combination of laboratory results and field observations by the Expedition physician suggest that women are capable of performing hard work at high altitude if they are in good condition and properly acclimatized.", "contents": "Response of women mountaineers to maximal exercise during hypoxia. Eight members of the American Women's Himalayan Expedition, ranging in age from 20-49, performed maximal exercise on a treadmill under normoxic and acute hypoxic (12.58% O2) conditions. Normoxic values for VO2 max were above average for all subjects and did not decline with age. The mean decrease in VO2 max (26.7%) during hypoxia was equivalent to that reported for younger males, which suggests that age was not a factor in response to hypoxia. Maximal heart rate, respiratory exchange ratio, oxygen pulse, and walk time were lower in hypoxia while ventilatory equivalent and blood lactate were higher. VEmax BTPS, was the same under both conditions. The combination of laboratory results and field observations by the Expedition physician suggest that women are capable of performing hard work at high altitude if they are in good condition and properly acclimatized."}
{"id": "PMID:486007", "title": "Effects of vaccination for Marek's disease on growth of a Marek's-virus-induced transplantable lymphoma in syngeneic and allogeneic chickens.", "content": "A study was made of the effects of vaccination with turkey herpesvirus on the growth of tumors following inoculation of lymphoma cells induced by Marek's disease virus (MDV). The cells used were from a transplantable MDV-induced lymphoid tumor (MDT-198) maintained by passaging in syngeneic hosts. Vaccination did not prevent progressive tumor growth or alter subsequent mortality among syngeneic recipients of MDT-198 cells. With allogeneic recipients, however, vaccination had a significant protective effect in that progressive tumor growth and mortality were markedly lowered. Both development of visceral lymphomas and atrophy of thymus and bursa were found at necropsy in both syngeneic and allogeneic hosts when progressive tumor growth occurred at the site of inoculation. The results suggest that it is unlikely that the primary and effective component of turkey-herpesvirus-induced protection against Marek's disease is directed against Marek's-disease-tumor-specific antigens.", "contents": "Effects of vaccination for Marek's disease on growth of a Marek's-virus-induced transplantable lymphoma in syngeneic and allogeneic chickens. A study was made of the effects of vaccination with turkey herpesvirus on the growth of tumors following inoculation of lymphoma cells induced by Marek's disease virus (MDV). The cells used were from a transplantable MDV-induced lymphoid tumor (MDT-198) maintained by passaging in syngeneic hosts. Vaccination did not prevent progressive tumor growth or alter subsequent mortality among syngeneic recipients of MDT-198 cells. With allogeneic recipients, however, vaccination had a significant protective effect in that progressive tumor growth and mortality were markedly lowered. Both development of visceral lymphomas and atrophy of thymus and bursa were found at necropsy in both syngeneic and allogeneic hosts when progressive tumor growth occurred at the site of inoculation. The results suggest that it is unlikely that the primary and effective component of turkey-herpesvirus-induced protection against Marek's disease is directed against Marek's-disease-tumor-specific antigens."}
{"id": "PMID:486013", "title": "Effect of cockpit temperature gradients on the validity of single-point measurements.", "content": "Dry bulb temperature was measured at six sites throughout seven sorties in F4E aircraft in a study of vertical and lateral cockpit temperature gradients designed to determine the validity of single-point measurements. The results show that both vertical and lateral gradients exist in F4E aircraft and that single-point measurements of Tdb close to the right shoulder show a bias of up to 4 degrees C in relation to mean cockpit dry bulb temperature derived from measurements at five sites. This bias may be removed by using the predictive relationships developed in this study. The relationship between black globe and dry bulb temperatures is also given for F4E aircraft flown in warm, sunny conditions.", "contents": "Effect of cockpit temperature gradients on the validity of single-point measurements. Dry bulb temperature was measured at six sites throughout seven sorties in F4E aircraft in a study of vertical and lateral cockpit temperature gradients designed to determine the validity of single-point measurements. The results show that both vertical and lateral gradients exist in F4E aircraft and that single-point measurements of Tdb close to the right shoulder show a bias of up to 4 degrees C in relation to mean cockpit dry bulb temperature derived from measurements at five sites. This bias may be removed by using the predictive relationships developed in this study. The relationship between black globe and dry bulb temperatures is also given for F4E aircraft flown in warm, sunny conditions."}
{"id": "PMID:486008", "title": "A streptomycin-dependent live pasteurella multocida type-3 vaccine for the prevention of fowl cholera in turkeys.", "content": "A live streptomycin-dependent Pasteurella multocida (Serotype 3) vaccine was found to protect turkeys orally and parenterally against homologous challenge.", "contents": "A streptomycin-dependent live pasteurella multocida type-3 vaccine for the prevention of fowl cholera in turkeys. A live streptomycin-dependent Pasteurella multocida (Serotype 3) vaccine was found to protect turkeys orally and parenterally against homologous challenge."}
{"id": "PMID:486009", "title": "Experimental Newcastle disease virus infections in three species of wild birds.", "content": "Three species of wild birds--the red-winged blackbird (Agelaius phoeniceus), an African weaver finch (Quelea quelea), and the sandhill crane (Grus canadensis)--were exposed to Newcastle disease virus (NDV) to determine susceptibility and host response. The NDV used were a vaccine strain (LaSota) and a viscerotropic velogenic strain (New York Parrot). Host response was monitored by virus isolation, signs of disease, and serologic response. Both the red-winged blackbirds and the quelea shed little virus and developed low antibody titers. A low mortality in the blackbirds did not appear to be due to ND alone. Vaccinated cranes were well protected against velogenic challenge, whereas unvaccinated cranes shed the velogenic virus from the cloaca for weeks. The ramifications of the low antibody titers produced in birds of two species are discussed, as well as the potential involvement of each species in transmission of the virus.", "contents": "Experimental Newcastle disease virus infections in three species of wild birds. Three species of wild birds--the red-winged blackbird (Agelaius phoeniceus), an African weaver finch (Quelea quelea), and the sandhill crane (Grus canadensis)--were exposed to Newcastle disease virus (NDV) to determine susceptibility and host response. The NDV used were a vaccine strain (LaSota) and a viscerotropic velogenic strain (New York Parrot). Host response was monitored by virus isolation, signs of disease, and serologic response. Both the red-winged blackbirds and the quelea shed little virus and developed low antibody titers. A low mortality in the blackbirds did not appear to be due to ND alone. Vaccinated cranes were well protected against velogenic challenge, whereas unvaccinated cranes shed the velogenic virus from the cloaca for weeks. The ramifications of the low antibody titers produced in birds of two species are discussed, as well as the potential involvement of each species in transmission of the virus."}
{"id": "PMID:486014", "title": "Influence of body temperature on nitrogen transport and decompression sickness in fish.", "content": "Incidence of lethal bends and intravascular bubbles has been studied in the eel (Anguilla anguila L.) submitted to hyperbaric air decompressions at temperatures of 17 and 27 degrees C. The fish was an accurate model to seek the nature of the inert gas transport limiting process (diffusion or perfusion) because an increase in temperature considerably influences the rate of perfusion whereas the properties of gases vary in relatively lower proportions. Qualitative and quantitative analysis of the results are consistent with the hypothesis of a diffusion limited nitrogen transport through the tissues of the decompressed eel.", "contents": "Influence of body temperature on nitrogen transport and decompression sickness in fish. Incidence of lethal bends and intravascular bubbles has been studied in the eel (Anguilla anguila L.) submitted to hyperbaric air decompressions at temperatures of 17 and 27 degrees C. The fish was an accurate model to seek the nature of the inert gas transport limiting process (diffusion or perfusion) because an increase in temperature considerably influences the rate of perfusion whereas the properties of gases vary in relatively lower proportions. Qualitative and quantitative analysis of the results are consistent with the hypothesis of a diffusion limited nitrogen transport through the tissues of the decompressed eel."}
{"id": "PMID:486015", "title": "Separation of the effects of raised skin and core temperature on performance of a pursuit rotor task.", "content": "Performance of a pursuit rotor task was studied during induced cycles of core temperature between limits of 37.9 degrees C and 38.5 degrees C. At each level of core temperature tested (37.9 degrees C, 38.5 degrees C, and 38.5 degrees C) performance was significantly worse during heating, when skin temperatures were high, than during cooling. The observed decrements were 13.6% at 37.9 degrees C, 16.0% at 38.2 degrees C and 18.1% at 38.5 degrees C. While it may be true that the performance changes were caused by changes in the level of skin temperature, the direction and rate of change of both core and skin temperatures may be important determinants of performance. The results are discussed in relation to current arousal theory.", "contents": "Separation of the effects of raised skin and core temperature on performance of a pursuit rotor task. Performance of a pursuit rotor task was studied during induced cycles of core temperature between limits of 37.9 degrees C and 38.5 degrees C. At each level of core temperature tested (37.9 degrees C, 38.5 degrees C, and 38.5 degrees C) performance was significantly worse during heating, when skin temperatures were high, than during cooling. The observed decrements were 13.6% at 37.9 degrees C, 16.0% at 38.2 degrees C and 18.1% at 38.5 degrees C. While it may be true that the performance changes were caused by changes in the level of skin temperature, the direction and rate of change of both core and skin temperatures may be important determinants of performance. The results are discussed in relation to current arousal theory."}
{"id": "PMID:486010", "title": "Prevention of egg transmission of Mycoplasma meleagridis by antibiotic treatment of naturally and experimentally infected turkey eggs.", "content": "Turkey eggs infected naturally and experimentally and treated with 1 mg of tylosin or gentamicin before incubation produced progeny with less infection with Mycoplasma meleagridis than did infected but untreated eggs. Higher doses of gentamicin eliminated infection, as indicated by an absence of M. meleagridis antibodies in poults tested at 3 weeks and retested at 5 weeks of age. The rate of reduction or elimination of egg transmission was correlated negatively with the incidence of M. meleagridis infection in untreated controls. Hatchability was reduced by gentamicin, especially at doses of 1.75 and 2 mg, whereas tylosin at 1 mg/egg improved hatchability.", "contents": "Prevention of egg transmission of Mycoplasma meleagridis by antibiotic treatment of naturally and experimentally infected turkey eggs. Turkey eggs infected naturally and experimentally and treated with 1 mg of tylosin or gentamicin before incubation produced progeny with less infection with Mycoplasma meleagridis than did infected but untreated eggs. Higher doses of gentamicin eliminated infection, as indicated by an absence of M. meleagridis antibodies in poults tested at 3 weeks and retested at 5 weeks of age. The rate of reduction or elimination of egg transmission was correlated negatively with the incidence of M. meleagridis infection in untreated controls. Hatchability was reduced by gentamicin, especially at doses of 1.75 and 2 mg, whereas tylosin at 1 mg/egg improved hatchability."}
{"id": "PMID:486016", "title": "Psychophysical assessment of angular vibration: comparison of vertical and roll vibrations.", "content": "A psychophysical matching experiment was conducted to compare the perceived intensity of roll vibration with the perceived intensity of Z-axis translational vibration. Seated subjects matched their perceptions of the intensity of Z-axis vibrations by adjusting the intensity of roll vibrations of the same frequency. The Z-axis stimuli were administered at seven frequencies (from 2.5 to 10 Hz) at each of three intensity levels: the 25-min Fatigue-Decreased Proficiency (FDP) level, the 1-hr FDP level, and the 2.5-hr FDP level, as defined in ISO Standard 2631-1974. The results showed that the acceleration of the roll matching response increased significantly as a function of both the intensity and the frequency of the stimulus vibrations. Since the stimuli were chosen from existing Z-axis equal intensity contours, the mean matching responses define equivalent contours of equal intensity for roll vibration. These results provide information on the relationship between Z-axis vibrations measured in translational acceleration units of G and roll vibrations measured in angular acceleration units of rad/s2. The determination of such relationships is essential for the development of improved vibration exposure criteria applicable to complex vibration environments.", "contents": "Psychophysical assessment of angular vibration: comparison of vertical and roll vibrations. A psychophysical matching experiment was conducted to compare the perceived intensity of roll vibration with the perceived intensity of Z-axis translational vibration. Seated subjects matched their perceptions of the intensity of Z-axis vibrations by adjusting the intensity of roll vibrations of the same frequency. The Z-axis stimuli were administered at seven frequencies (from 2.5 to 10 Hz) at each of three intensity levels: the 25-min Fatigue-Decreased Proficiency (FDP) level, the 1-hr FDP level, and the 2.5-hr FDP level, as defined in ISO Standard 2631-1974. The results showed that the acceleration of the roll matching response increased significantly as a function of both the intensity and the frequency of the stimulus vibrations. Since the stimuli were chosen from existing Z-axis equal intensity contours, the mean matching responses define equivalent contours of equal intensity for roll vibration. These results provide information on the relationship between Z-axis vibrations measured in translational acceleration units of G and roll vibrations measured in angular acceleration units of rad/s2. The determination of such relationships is essential for the development of improved vibration exposure criteria applicable to complex vibration environments."}
{"id": "PMID:486019", "title": "Temperature changes during exercise measured by thermography.", "content": "The present study explores the dynamic changes of surface temperatures associated with peripheral vascular shifts during and following hand gripping, one-legged ergometric, and running exercise. An AGA 680 thermovision system, incorporating a Bolex 16-mm camera and displaying 10-colored isotherms on a slave monitor, generated synchronous infrared (IR) movies at 16 frame/s. Incremental increases in surface skin temperatures (Ts) were observed while gripping at 20, 50, and 80% of maximum with a peak rise of 1.7 degrees C. Thermographic recorded Ts changes during dynamic one-legged exercise were higher in the exercising leg than the resting leg. Surface temperatures dropped in both limbs following exercise. The advantages of the use of thermography during transient thermal states, as produced by dynamic exercise, is supported by comparative Ts data taken from thermograms and thermistors.", "contents": "Temperature changes during exercise measured by thermography. The present study explores the dynamic changes of surface temperatures associated with peripheral vascular shifts during and following hand gripping, one-legged ergometric, and running exercise. An AGA 680 thermovision system, incorporating a Bolex 16-mm camera and displaying 10-colored isotherms on a slave monitor, generated synchronous infrared (IR) movies at 16 frame/s. Incremental increases in surface skin temperatures (Ts) were observed while gripping at 20, 50, and 80% of maximum with a peak rise of 1.7 degrees C. Thermographic recorded Ts changes during dynamic one-legged exercise were higher in the exercising leg than the resting leg. Surface temperatures dropped in both limbs following exercise. The advantages of the use of thermography during transient thermal states, as produced by dynamic exercise, is supported by comparative Ts data taken from thermograms and thermistors."}
{"id": "PMID:486020", "title": "Effect of physical training at moderate altitude (1850 m) on hypoxic tolerance.", "content": "Effect of systematic physical training at moderate altitude (1850 m) on hypoxic tolerance was estimated on a group of young soldiers, by determining the Time of Useful Consciousness (TUC) at a simulated altitude of 7620 m in a hypobaric chamber together with the Critical Flicker Frequency (CFF) test. The subjects, after initial testing at Delhi (near sea level) were taken to an altitude of 1850 m and divided into two groups. One group was given systematic endurance physical training for 8 weeks, while the other group served as control. The subjects were then taken to an altitude of 3500 m and kept at that altitude for 4 weeks, after which they were brought back to Delhi and TUC was estimated again within 2 d. The results indicate that endurance physical training at moderate altitude improved hypoxic tolerance and the central nervous system activity under hypoxia.", "contents": "Effect of physical training at moderate altitude (1850 m) on hypoxic tolerance. Effect of systematic physical training at moderate altitude (1850 m) on hypoxic tolerance was estimated on a group of young soldiers, by determining the Time of Useful Consciousness (TUC) at a simulated altitude of 7620 m in a hypobaric chamber together with the Critical Flicker Frequency (CFF) test. The subjects, after initial testing at Delhi (near sea level) were taken to an altitude of 1850 m and divided into two groups. One group was given systematic endurance physical training for 8 weeks, while the other group served as control. The subjects were then taken to an altitude of 3500 m and kept at that altitude for 4 weeks, after which they were brought back to Delhi and TUC was estimated again within 2 d. The results indicate that endurance physical training at moderate altitude improved hypoxic tolerance and the central nervous system activity under hypoxia."}
{"id": "PMID:486021", "title": "Hyperventilation in aircrew: a review.", "content": "The causes and effects of hyperventilation, relevant to the flight environment, have been reviewed and one case history is presented. Methods of investigating in-flight hyperventilation are discussed.", "contents": "Hyperventilation in aircrew: a review. The causes and effects of hyperventilation, relevant to the flight environment, have been reviewed and one case history is presented. Methods of investigating in-flight hyperventilation are discussed."}
{"id": "PMID:486022", "title": "Epileptic-type convulsions and magnesium deficiency.", "content": "Immediately following 4 h of continuous exercise at +/- 45% Vo2max in heat, a 23-year-old, well-trained man displayed epileptic-type convulsions. One week preceeding this incident, he completed an identical work test successfully under room temperature conditions. An assessment of his physiological and biochemical results indicated only one abnormality: during exercise in heat, an abnormally low serum magnesium concentration prevailed for most of the test. Treatment with phenobarb and magnesium chloride enteric tablets (\"Slow Mag\", 2 x 535 mg/d) reversed the biochemical abnormality. After checking his resting serum magnesium, the subject subsequently heat acclimatized and repeated similar treadmill tests as before without any ill effects.", "contents": "Epileptic-type convulsions and magnesium deficiency. Immediately following 4 h of continuous exercise at +/- 45% Vo2max in heat, a 23-year-old, well-trained man displayed epileptic-type convulsions. One week preceeding this incident, he completed an identical work test successfully under room temperature conditions. An assessment of his physiological and biochemical results indicated only one abnormality: during exercise in heat, an abnormally low serum magnesium concentration prevailed for most of the test. Treatment with phenobarb and magnesium chloride enteric tablets (\"Slow Mag\", 2 x 535 mg/d) reversed the biochemical abnormality. After checking his resting serum magnesium, the subject subsequently heat acclimatized and repeated similar treadmill tests as before without any ill effects."}
{"id": "PMID:486024", "title": "Measuring instrument for the determination of the oxygen consumption of scuba divers in open waters.", "content": "An apparatus is described for the estimation of incremental oxygen consumption and carbon-dioxide production of SCUBA divers working in the field. Air is supplied to the diver in discrete volumes, and aliquots of respired air collected from each discrete time interval.", "contents": "Measuring instrument for the determination of the oxygen consumption of scuba divers in open waters. An apparatus is described for the estimation of incremental oxygen consumption and carbon-dioxide production of SCUBA divers working in the field. Air is supplied to the diver in discrete volumes, and aliquots of respired air collected from each discrete time interval."}
{"id": "PMID:486070", "title": "Inheritance of isozyme variation and heterozygosity in Pinus ponderosa.", "content": "Techniques are presented to detect 23 isozyme loci in the long-lived perennial plant, ponderosa pine. Meiotically derived megagametophyte from seeds is used to examine directly the segregation of allelic variants. Approximately seven seeds were initially examined for 12 enzymes from each of 47 trees from ten stands throughout the northern Rocky Mountain region. Additional seeds were also examined from selected families to confirm the inheritance of observed electrophoretic variants at 13 polymorphic loci and to estimate linkage relationship. Significant norandom segregation was consistently detected for three pairs of loci: ADH-1:AAT-2, ADH-1:PGI-1, and LAP-2:6PG-1. Preliminary estimates of population parameters reveal a relatively high average heterozygosity (H = 0.123). This is partitioned into a high amont of genetic variation within local stands, with only approximately 12% of the total heterozygosity resulting from genic difference between stands.", "contents": "Inheritance of isozyme variation and heterozygosity in Pinus ponderosa. Techniques are presented to detect 23 isozyme loci in the long-lived perennial plant, ponderosa pine. Meiotically derived megagametophyte from seeds is used to examine directly the segregation of allelic variants. Approximately seven seeds were initially examined for 12 enzymes from each of 47 trees from ten stands throughout the northern Rocky Mountain region. Additional seeds were also examined from selected families to confirm the inheritance of observed electrophoretic variants at 13 polymorphic loci and to estimate linkage relationship. Significant norandom segregation was consistently detected for three pairs of loci: ADH-1:AAT-2, ADH-1:PGI-1, and LAP-2:6PG-1. Preliminary estimates of population parameters reveal a relatively high average heterozygosity (H = 0.123). This is partitioned into a high amont of genetic variation within local stands, with only approximately 12% of the total heterozygosity resulting from genic difference between stands."}
{"id": "PMID:486071", "title": "Genetic polymorphism of the major parotid salivary glycoprotein (Gl) with linkage to the genes for Pr, Db, and Pa.", "content": "Genetic polymorphism of the major glycoprotein (Gl) found in parotid saliva is determined by autosomal inheritance of one unexpressed and four expressed alleles. This hypothesis is supported by studies in 41 white families including 146 children. For 143 randomly collected salivas from whites and 82 randomly collected salivas from blacks, maximum likelihood estimates of the gene frequencies are as follows: for whites, Gl1 = 0.742, Gl2 = 0.040, Gl3 = 0.155, Gl4 = 0.017, Gl0 = 0.46; for blacks, Gl1 = 0.459, Gl2 = 0.050, Gl3 = 0.337, Gl4 = 0.044, Gl0 = 0.110. There is strong evidence for linkage of Gl/Pr (seven families, lod score at theta = 0 is 5.24) and G1/Db (eight families, lod score at theta = 0 is 4.45). The allelic products of Gl show evidence for linkage disequilibrium with the products of the Pr, Db, and Pa loci (P less than 0.0005). On the basis of varying degrees of linkage disequilibrium, Gl may be closer to Db than to Pr or Pa and on the \"outside\" of Db with respect to Pr or Pa. Amino acid analyses of Gl 1 and Gl 4 proteins show strong resemblances in composition to the major basic glycoprotein and the acidic proline-rich proteins of parotid saliva described by other workers. The polymorphic forms of the Gl proteins show microheterogeneity due to variability in charge and molecular weight. The electrophoretic polymorphism appears to be determined by apparent differences in molecular weights between the Gl proteins.", "contents": "Genetic polymorphism of the major parotid salivary glycoprotein (Gl) with linkage to the genes for Pr, Db, and Pa. Genetic polymorphism of the major glycoprotein (Gl) found in parotid saliva is determined by autosomal inheritance of one unexpressed and four expressed alleles. This hypothesis is supported by studies in 41 white families including 146 children. For 143 randomly collected salivas from whites and 82 randomly collected salivas from blacks, maximum likelihood estimates of the gene frequencies are as follows: for whites, Gl1 = 0.742, Gl2 = 0.040, Gl3 = 0.155, Gl4 = 0.017, Gl0 = 0.46; for blacks, Gl1 = 0.459, Gl2 = 0.050, Gl3 = 0.337, Gl4 = 0.044, Gl0 = 0.110. There is strong evidence for linkage of Gl/Pr (seven families, lod score at theta = 0 is 5.24) and G1/Db (eight families, lod score at theta = 0 is 4.45). The allelic products of Gl show evidence for linkage disequilibrium with the products of the Pr, Db, and Pa loci (P less than 0.0005). On the basis of varying degrees of linkage disequilibrium, Gl may be closer to Db than to Pr or Pa and on the \"outside\" of Db with respect to Pr or Pa. Amino acid analyses of Gl 1 and Gl 4 proteins show strong resemblances in composition to the major basic glycoprotein and the acidic proline-rich proteins of parotid saliva described by other workers. The polymorphic forms of the Gl proteins show microheterogeneity due to variability in charge and molecular weight. The electrophoretic polymorphism appears to be determined by apparent differences in molecular weights between the Gl proteins."}
{"id": "PMID:486072", "title": "Distinguishing allozymes and isozymes of phosphoglucoisomerases by electrophoretic comparisons of pollen and somatic tissues.", "content": "The different electrophoretic patterns of dimeric phosphoglucoisomerases extracted from haploid pollen and diploid somatic tissues of plants may be used to distinguish allozymes and isozymes. The analysis depends on the presence of two alleles at each locus in somatic tissues but only one or the other allele in pollen grains. Consequently, in heterozygotes, heterodimeric allozymes can be identified because they are formed in stems and leaves but not in pollen. The procedure is described in enzymes extracted from the diploid annual plant Clarkia dudleyana, which possesses three gene loci for PGI subunits. Comparison of the electrophoretic patterns of stem and pollen extracts makes it possible in many cases to identify allelic state without breeding tests. The technique also is likely to be useful in the interpretation of zymograms of other multimeric enzymes coded by more than one gene locus.", "contents": "Distinguishing allozymes and isozymes of phosphoglucoisomerases by electrophoretic comparisons of pollen and somatic tissues. The different electrophoretic patterns of dimeric phosphoglucoisomerases extracted from haploid pollen and diploid somatic tissues of plants may be used to distinguish allozymes and isozymes. The analysis depends on the presence of two alleles at each locus in somatic tissues but only one or the other allele in pollen grains. Consequently, in heterozygotes, heterodimeric allozymes can be identified because they are formed in stems and leaves but not in pollen. The procedure is described in enzymes extracted from the diploid annual plant Clarkia dudleyana, which possesses three gene loci for PGI subunits. Comparison of the electrophoretic patterns of stem and pollen extracts makes it possible in many cases to identify allelic state without breeding tests. The technique also is likely to be useful in the interpretation of zymograms of other multimeric enzymes coded by more than one gene locus."}
{"id": "PMID:486073", "title": "DNA of Akodon (Rodentia, Cricetidae). I. Biophysical characterization.", "content": "Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699-1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The Tm values determined in 1 x SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2-43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.", "contents": "DNA of Akodon (Rodentia, Cricetidae). I. Biophysical characterization. Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699-1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The Tm values determined in 1 x SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2-43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability."}
{"id": "PMID:486074", "title": "Biochemical characterization of \"LAP,\" a polymorphic aminopeptidase from the blue mussel, Mytilus edulis.", "content": "A genetically variable naphthylamidase enzyme, previously described as \"leucine aminopeptidase,\" was purified approximately fiftyfold, and its biochemical properties were investigated. The enzyme was renamed \"aminopeptidase I.\" Substrate affinities demonstrate that it is an alpha-aminoacyl peptide hydrolase (E.C. 3.4.11.-). Aminopeptidase I had a monomer molecular weight of 65--68,000, average of pI of pH 4.88, and broad pH optima between 6.5 and 8.0. The enzyme was inactivated rapidly between 40 and 50 C. Antibodies from purified enzyme did not cross-react with other naphthylamidases, but aminopeptidase I activity was inhibited by immune serum. The enzyme exhibited highest naphthylamidase activity for aromatic and hydrophobic aminoacyl naphthylamides. Aminopeptidase activity was highest for aromatic and hydrophobic N-terminal residues of tripeptides. Certain divalent metal cations, p-OH-mercuribenzoate, and N-ethylmaleimide were strongly inhibitory while chelating agents activated the enzyme.", "contents": "Biochemical characterization of \"LAP,\" a polymorphic aminopeptidase from the blue mussel, Mytilus edulis. A genetically variable naphthylamidase enzyme, previously described as \"leucine aminopeptidase,\" was purified approximately fiftyfold, and its biochemical properties were investigated. The enzyme was renamed \"aminopeptidase I.\" Substrate affinities demonstrate that it is an alpha-aminoacyl peptide hydrolase (E.C. 3.4.11.-). Aminopeptidase I had a monomer molecular weight of 65--68,000, average of pI of pH 4.88, and broad pH optima between 6.5 and 8.0. The enzyme was inactivated rapidly between 40 and 50 C. Antibodies from purified enzyme did not cross-react with other naphthylamidases, but aminopeptidase I activity was inhibited by immune serum. The enzyme exhibited highest naphthylamidase activity for aromatic and hydrophobic aminoacyl naphthylamides. Aminopeptidase activity was highest for aromatic and hydrophobic N-terminal residues of tripeptides. Certain divalent metal cations, p-OH-mercuribenzoate, and N-ethylmaleimide were strongly inhibitory while chelating agents activated the enzyme."}
{"id": "PMID:486075", "title": "Expression of PGK-A in the Australian brush-tailed possum, Trichosurus vulpecula (Kerr), consistent with paternal X inactivation.", "content": "An extensive survey of erythrocytes of marsupials other than kangaroos for electrophoretic variation if X-linked enzymes revealed two rare PGK-A phenotypes in the phalangerid Trichosurus vupecula and one in Trichosurus caninus. Four putatively heterozygous females expressed only the variant allelic isozyme in some tissues but expressed a trace of the normal isozyme in others. A putatively hemizygous male expressed only the variant isozyme in all tissues. The phenotypic patterns were consistent with those observed in kangaroos known to exhibit partial or complete parternal X inactivation in cells of females. Tow of the T. vulpecula were a mother and her female pouch young, further suggesting that paternal X inactivation occurs in T. vulpecula. This peculiar mechanism of dosage compensation may not be restricted to kangaroos.", "contents": "Expression of PGK-A in the Australian brush-tailed possum, Trichosurus vulpecula (Kerr), consistent with paternal X inactivation. An extensive survey of erythrocytes of marsupials other than kangaroos for electrophoretic variation if X-linked enzymes revealed two rare PGK-A phenotypes in the phalangerid Trichosurus vupecula and one in Trichosurus caninus. Four putatively heterozygous females expressed only the variant allelic isozyme in some tissues but expressed a trace of the normal isozyme in others. A putatively hemizygous male expressed only the variant isozyme in all tissues. The phenotypic patterns were consistent with those observed in kangaroos known to exhibit partial or complete parternal X inactivation in cells of females. Tow of the T. vulpecula were a mother and her female pouch young, further suggesting that paternal X inactivation occurs in T. vulpecula. This peculiar mechanism of dosage compensation may not be restricted to kangaroos."}
{"id": "PMID:486076", "title": "Evolution of fraction 1 protein in the genus Lycopersicon.", "content": "The large- and small-subunit polypeptide composition of fraction 1 protein contained in seven species of Lycopersicon and Solanum pennellii was determined by electrofocusing. The eight species of protein had large subunits composed of three polypeptides separated by about 0.05 pH unit, but there was no difference in the isoelectric points of the clusters of three polypeptides. By this criterion, no surviving mutations have appeared in the extranuclear DNA coding for the cluster of large-subunit polypeptides during a period of evolution which generated the eight species of plants. The genus Lycopersicon appears to be much younger than its sister genus Nicotiana in the family Solanaceae, where four types of polypeptide clusters have evolved. Three different small-subunit polypeptides whose isoelectric points are coded by nuclear DNA have arisen among the seven Lycopersicon species, and L. hirsutum and S. pennellii have proteins containing single polypeptides and are therefore considered older than L. chilense, L. chimielewskii, and L. parviflorum, whose proteins contain two polypeptides. L. cheesemanii, L. pimpinellifolium, and L. esculentum (and probably L. peruvianum) seem to be the most recently evolved species since their fraction 1 proteins have small subunits composed of three polypeptides.", "contents": "Evolution of fraction 1 protein in the genus Lycopersicon. The large- and small-subunit polypeptide composition of fraction 1 protein contained in seven species of Lycopersicon and Solanum pennellii was determined by electrofocusing. The eight species of protein had large subunits composed of three polypeptides separated by about 0.05 pH unit, but there was no difference in the isoelectric points of the clusters of three polypeptides. By this criterion, no surviving mutations have appeared in the extranuclear DNA coding for the cluster of large-subunit polypeptides during a period of evolution which generated the eight species of plants. The genus Lycopersicon appears to be much younger than its sister genus Nicotiana in the family Solanaceae, where four types of polypeptide clusters have evolved. Three different small-subunit polypeptides whose isoelectric points are coded by nuclear DNA have arisen among the seven Lycopersicon species, and L. hirsutum and S. pennellii have proteins containing single polypeptides and are therefore considered older than L. chilense, L. chimielewskii, and L. parviflorum, whose proteins contain two polypeptides. L. cheesemanii, L. pimpinellifolium, and L. esculentum (and probably L. peruvianum) seem to be the most recently evolved species since their fraction 1 proteins have small subunits composed of three polypeptides."}
{"id": "PMID:486077", "title": "Segregation of the isozymes of flax genotrophs.", "content": "The segregation of isozymes of peroxidase and acid phosphatase in progenies of crosses between large (L) and small (S and L6) flax genotrophs has been determined. The peroxidase isozymes segregated as expected on a simple Mendelian model with a dominant and a recessive allele and with the L genotroph being a homozygous dominant. All the peroxidase isozymes which differed segregated together, so the isozymes are controlled by either a single locus or closely linked loci. The acid phosphatase isozymes in the F1 were all L type, but the segregations observed in the F2 were not always consistent with a simple Mendelian model.", "contents": "Segregation of the isozymes of flax genotrophs. The segregation of isozymes of peroxidase and acid phosphatase in progenies of crosses between large (L) and small (S and L6) flax genotrophs has been determined. The peroxidase isozymes segregated as expected on a simple Mendelian model with a dominant and a recessive allele and with the L genotroph being a homozygous dominant. All the peroxidase isozymes which differed segregated together, so the isozymes are controlled by either a single locus or closely linked loci. The acid phosphatase isozymes in the F1 were all L type, but the segregations observed in the F2 were not always consistent with a simple Mendelian model."}
{"id": "PMID:486078", "title": "Labelling of the intramembranous region of the major sialoglycoprotein of human erythrocytes with a photosensitive hydrophobic probe.", "content": "Human erythrocyte membranes were incubated with the photosensitive hydrophobic reagent 1-azido-r-iodo[3H]benzene and the mixture was irradiated. The major sialoglycoprotein was then isolated and the labelled polypeptide subjected to proteolytic dissection. Characterization of the purified tryptic and chymotryptic peptides show that the probe is covalently attached only to the transmembrane region of the protein. This labelling pattern is discussed in relation to the use of such reagents for the identification of segments of membrane proteins exposed to the hydrophobic millieu of the membrane.", "contents": "Labelling of the intramembranous region of the major sialoglycoprotein of human erythrocytes with a photosensitive hydrophobic probe. Human erythrocyte membranes were incubated with the photosensitive hydrophobic reagent 1-azido-r-iodo[3H]benzene and the mixture was irradiated. The major sialoglycoprotein was then isolated and the labelled polypeptide subjected to proteolytic dissection. Characterization of the purified tryptic and chymotryptic peptides show that the probe is covalently attached only to the transmembrane region of the protein. This labelling pattern is discussed in relation to the use of such reagents for the identification of segments of membrane proteins exposed to the hydrophobic millieu of the membrane."}
{"id": "PMID:486079", "title": "Studies on horseradish peroxidase in dimethyl sulphoxide/water mixtures. The activation of hydrogen peroxide and the binding of fluoride.", "content": "We studied the variation in spectra and in reactivity towards H2O2 of solutions of horseradish peroxidase in dimethyl sulphoxide/water mixtures, obtained by diluting stock solutions of the enzyme in either water or dimethyl sulphoxide, and assayed the enzyme activity and studied the binding of F- by the peroxidase in 65% (v/v) dimethyl sulphoxide. A broadly similar pattern of changes is observed whether one starts from water or from dimethyl sulphoxide; the changes are essentially reversible, though hysteresis is observed. When the dimethyl sulphoxide content of the solvent mixture is increased, the peroxidase retains its ability to activate H2O2 up to 74% (v/v) dimethyl sulphoxide. The peroxidase in 65% (v/v) dimethyl sulphoxide binds F- together with a proton (or the equivalent loss of HO-), as already established for aqueous solutions. We point out that the occurrence in such solutions of both the ability to activate H2O2 and the inability to bind F- without taking up H+ or losing HO- supports the proposed mechanism for activating H202, whereby the protein binds the substrate in the form of the much more reactive HO2-.", "contents": "Studies on horseradish peroxidase in dimethyl sulphoxide/water mixtures. The activation of hydrogen peroxide and the binding of fluoride. We studied the variation in spectra and in reactivity towards H2O2 of solutions of horseradish peroxidase in dimethyl sulphoxide/water mixtures, obtained by diluting stock solutions of the enzyme in either water or dimethyl sulphoxide, and assayed the enzyme activity and studied the binding of F- by the peroxidase in 65% (v/v) dimethyl sulphoxide. A broadly similar pattern of changes is observed whether one starts from water or from dimethyl sulphoxide; the changes are essentially reversible, though hysteresis is observed. When the dimethyl sulphoxide content of the solvent mixture is increased, the peroxidase retains its ability to activate H2O2 up to 74% (v/v) dimethyl sulphoxide. The peroxidase in 65% (v/v) dimethyl sulphoxide binds F- together with a proton (or the equivalent loss of HO-), as already established for aqueous solutions. We point out that the occurrence in such solutions of both the ability to activate H2O2 and the inability to bind F- without taking up H+ or losing HO- supports the proposed mechanism for activating H202, whereby the protein binds the substrate in the form of the much more reactive HO2-."}
{"id": "PMID:486080", "title": "Use of photosensitive hydrophobic probes to label the membrane of the human erythrocyte.", "content": "Two photosensitive hydrophobic probes, azido [3H]benzene and 1-azido-4-iodo[3H]-benzene, have been compared for their effectiveness in labelling, selectively, the intramembranous domains of lipid and proteins. Both partition preferentially into the lipid bilayer and, upon irradiation, covalently attach to both phospholipids and membrane proteins; the more extrinsic polypeptides have a significantly lower specific radioactivity than that of the intrinsic species. Proteolytic experiments also reveal higher labelling of intramembranous regions of the proteins. Consistently, the iodinated form of the probe showed the greater preference for the non-polar phase and a higher degree of selectivity for labelling hydrophobic regions. The results also suggest that penetration through the annulus of tightly bound lipid surrounding integral proteins occurs readily.", "contents": "Use of photosensitive hydrophobic probes to label the membrane of the human erythrocyte. Two photosensitive hydrophobic probes, azido [3H]benzene and 1-azido-4-iodo[3H]-benzene, have been compared for their effectiveness in labelling, selectively, the intramembranous domains of lipid and proteins. Both partition preferentially into the lipid bilayer and, upon irradiation, covalently attach to both phospholipids and membrane proteins; the more extrinsic polypeptides have a significantly lower specific radioactivity than that of the intrinsic species. Proteolytic experiments also reveal higher labelling of intramembranous regions of the proteins. Consistently, the iodinated form of the probe showed the greater preference for the non-polar phase and a higher degree of selectivity for labelling hydrophobic regions. The results also suggest that penetration through the annulus of tightly bound lipid surrounding integral proteins occurs readily."}
{"id": "PMID:486081", "title": "Studies on haemin in dimethyl sulphoxide/water mixtures.", "content": "The nature of the complexes and equilibria shown by solutions of protohaemin in dimethyl sulphoxide/water mixtures and in the presence of acid and base were studied by u.v.-visible spectrophotometry. In neutral solutions containing from 40 to 100% dimethyl sulphoxide, haemin is present as a monomeric complex in which the Cl-ion is not coordinated. Only a single pH-dependent equilibrium pK12 is observed over the range 40-80% dimethylsulphoxide, corresponding to formation of the mu-oxo dimer. As the dimethyl sulphoxide content is lowered below 35%, so the single equilibrium (pK12) is replaced by two equilibria (pK1 and pK2); with solutions of 5 microM-haemin, pK1 decreases (from pK12 7.55 in 65% dimethyl sulphoxide to pK1 approx. 1.5 in 0.01% dimethyl sulphoxide), whereas pK2 hardly changes (from pK12 7.55 in 65% to pK2 approx. 7.5 in 0.01%).", "contents": "Studies on haemin in dimethyl sulphoxide/water mixtures. The nature of the complexes and equilibria shown by solutions of protohaemin in dimethyl sulphoxide/water mixtures and in the presence of acid and base were studied by u.v.-visible spectrophotometry. In neutral solutions containing from 40 to 100% dimethyl sulphoxide, haemin is present as a monomeric complex in which the Cl-ion is not coordinated. Only a single pH-dependent equilibrium pK12 is observed over the range 40-80% dimethylsulphoxide, corresponding to formation of the mu-oxo dimer. As the dimethyl sulphoxide content is lowered below 35%, so the single equilibrium (pK12) is replaced by two equilibria (pK1 and pK2); with solutions of 5 microM-haemin, pK1 decreases (from pK12 7.55 in 65% dimethyl sulphoxide to pK1 approx. 1.5 in 0.01% dimethyl sulphoxide), whereas pK2 hardly changes (from pK12 7.55 in 65% to pK2 approx. 7.5 in 0.01%)."}
{"id": "PMID:486082", "title": "The variation with age of the structure of chromatin in three cell types from rat liver.", "content": "The organization of chromatin in three rat liver nuclear populations, namely diploid stromal, diploid parenchymal, and tetraploid parenchymal nuclei, which were separated by zonal centrifugation, was studied by digestion with micrococcal nuclease and pancreatic deoxyribonuclease in 3-week-old rats in which the parenchymal cells contain diploid nuclei and in 2-and 4-month-old rats with a high proportion of tetraploid nuclei. Digestion by micrococcal nuclease allowed the estimation of DNA-repeat length in chromatin. Parenchymal nuclei have shorter repeat length than stromal nuclei and DNA-repeat length increases with the age in all three nuclei populations. The kinetics of digestion by micrococcal nuclease showed that nuclei with shorter repeat length are more sensitive to micrococcal nuclease and that the sensitivity of chromatin decreases with age for all the types of nuclei in this study. The kinetics of digestion by pancreatic deoxyribonuclease showed that sensitivity of chromatin is related to the repeat length and that the sensitivity decreases with the ages.", "contents": "The variation with age of the structure of chromatin in three cell types from rat liver. The organization of chromatin in three rat liver nuclear populations, namely diploid stromal, diploid parenchymal, and tetraploid parenchymal nuclei, which were separated by zonal centrifugation, was studied by digestion with micrococcal nuclease and pancreatic deoxyribonuclease in 3-week-old rats in which the parenchymal cells contain diploid nuclei and in 2-and 4-month-old rats with a high proportion of tetraploid nuclei. Digestion by micrococcal nuclease allowed the estimation of DNA-repeat length in chromatin. Parenchymal nuclei have shorter repeat length than stromal nuclei and DNA-repeat length increases with the age in all three nuclei populations. The kinetics of digestion by micrococcal nuclease showed that nuclei with shorter repeat length are more sensitive to micrococcal nuclease and that the sensitivity of chromatin decreases with age for all the types of nuclei in this study. The kinetics of digestion by pancreatic deoxyribonuclease showed that sensitivity of chromatin is related to the repeat length and that the sensitivity decreases with the ages."}
{"id": "PMID:486083", "title": "The relationship of detergent-solubilization plasma-membrane components of rabbit alveolar macrophages to an isolated inhibitor of phagocytosis.", "content": "1. A plasma-membrane fraction prepared from rabbit alveolar macrophages by hyposmotic borate lysis is described. 2. Rabbit lung lavages, containing a glycoprotein inhibitor of phagocytosis, may be fractionated by preparative isoelectric focusing in the presence of Triton X-100. 3. Chemical analysis indicates that the glycoproteins of the lung lavage contain sialic acid, fucose, mannose, galactose, hexosamine and appreciable quantities of glucose. 4. The relationship of macrophage membrane glycoproteins, solubilized with Triton X-100 in the presence of borate, to the lung lavage glycoproteins is demonstrated immunoelectrophoretically.", "contents": "The relationship of detergent-solubilization plasma-membrane components of rabbit alveolar macrophages to an isolated inhibitor of phagocytosis. 1. A plasma-membrane fraction prepared from rabbit alveolar macrophages by hyposmotic borate lysis is described. 2. Rabbit lung lavages, containing a glycoprotein inhibitor of phagocytosis, may be fractionated by preparative isoelectric focusing in the presence of Triton X-100. 3. Chemical analysis indicates that the glycoproteins of the lung lavage contain sialic acid, fucose, mannose, galactose, hexosamine and appreciable quantities of glucose. 4. The relationship of macrophage membrane glycoproteins, solubilized with Triton X-100 in the presence of borate, to the lung lavage glycoproteins is demonstrated immunoelectrophoretically."}
{"id": "PMID:486084", "title": "Localization and verification by synthesis of five antigenic sites of bovine serum albumin.", "content": "Recently we have shown that the major antigenic sites of bovine serum albumin exhibit functional equivalence progessively increasing with the time at which antibodies are obtained after the first immunization. Analysis of our recent immunochemical findings and the known covalent structure of bovine serum albumin have enabled us to predict the locations of five antigenic sites of bovine serum albumin. The predicted locations were synthesized, and immunochemical studies with late-course antisera showed them to constitute antigenic sites of native bovine serum albumin.", "contents": "Localization and verification by synthesis of five antigenic sites of bovine serum albumin. Recently we have shown that the major antigenic sites of bovine serum albumin exhibit functional equivalence progessively increasing with the time at which antibodies are obtained after the first immunization. Analysis of our recent immunochemical findings and the known covalent structure of bovine serum albumin have enabled us to predict the locations of five antigenic sites of bovine serum albumin. The predicted locations were synthesized, and immunochemical studies with late-course antisera showed them to constitute antigenic sites of native bovine serum albumin."}
{"id": "PMID:486085", "title": "Structural defects in rat liver deoxyribonucleic acid. Endogenous single-strained regions in comparison with damage induced in vivo by a carcinogen.", "content": "Rat liver DNA may be separated into two fractions by stepwise elution from benzoylated-DEAE-cellulose with NaCl and caffeine solutions respectively. Other studies using bacterical and yeast DNA suggested that the first fraction contains native DNA, whereas the second may exhibit some degree of single-stranded character. In the present experiments, chromatography of DNA was monitored by labelling in vivo with [methyl-3H]thymidine in rats previously subjected to partial hepatectomy. In animals killed up to 1 h after thymidine injection, radioactivity eluted in the second fraction was inversely related to the incorporation time, being greatest when animals were killed 10 min after radioisotope injection. However, for most experiments, animals were allowed to survive 2-4 weeks after surgery before use, analysis being made on non-dividing DNA. Under these conditions, the proportion of caffeine-eluted DNA was decreased by subjecting the preparation to shear, before chromatography. A procedure that resulted in 12% of the recovered radioactivity being eluted with caffeine was adopted for experiments involving comparisons of the two DNA fractions. Under these conditions, cross-contamination could be detected by rechromatography, but this did not preclude distinction being made between the two fractions in terms of DNA structure. NaCl-eluted DNA did not bind to nitrocellulose filters. Caffeine-eluted DNA was retained by the filters and released by washing with 3mM-Tris/HCl,pH9.4. The fractions did not differ in terms of isopycnic centrifugation in CsCl. The NaCl-eluted fraction migrated as a single band in polyacrylamide gels, and this pattern was not modified by prior digestion with Neurospora crassa endonuclease. In contrast, caffeine-eluted DNA contained a minor component having a wide molecular-weight distribution and was subject to limited digestion by the endonuclease. The kinetics of denaturation of NaCi-eluted DNA in the presence of formaldehyde, in common with unfractionated DNA, were consistent with double-stranded structure. The same analysis of caffeine-eluted DNA revealed structural abnormality equivalent to two defects per 10000 base-pairs. The data are consistent with the minor fraction of rat liver DNA, separated by using benzoylated-DEAE-cellulose, containing regions of local denaturation. We previously showed that administration of the hepatocarcinogen dimethylnitrosamine is associated with an increase in the proportion of caffeine-eluted DNA. In terms of most analysis, differences between DNA fraction from nitrosamine-treated rats were similar to differences exhibited by preparations from control animals. However, structural analysis using denaturation kinetics indicated defects in both the NaCl- and caffeine-eluted DNA isolated from nitrosamine-treated rats. The two fractions differed from each other in that caffeine-eluted DNA exhibited a degree of structural damage far greater than that detected in any preparation from control animals...", "contents": "Structural defects in rat liver deoxyribonucleic acid. Endogenous single-strained regions in comparison with damage induced in vivo by a carcinogen. Rat liver DNA may be separated into two fractions by stepwise elution from benzoylated-DEAE-cellulose with NaCl and caffeine solutions respectively. Other studies using bacterical and yeast DNA suggested that the first fraction contains native DNA, whereas the second may exhibit some degree of single-stranded character. In the present experiments, chromatography of DNA was monitored by labelling in vivo with [methyl-3H]thymidine in rats previously subjected to partial hepatectomy. In animals killed up to 1 h after thymidine injection, radioactivity eluted in the second fraction was inversely related to the incorporation time, being greatest when animals were killed 10 min after radioisotope injection. However, for most experiments, animals were allowed to survive 2-4 weeks after surgery before use, analysis being made on non-dividing DNA. Under these conditions, the proportion of caffeine-eluted DNA was decreased by subjecting the preparation to shear, before chromatography. A procedure that resulted in 12% of the recovered radioactivity being eluted with caffeine was adopted for experiments involving comparisons of the two DNA fractions. Under these conditions, cross-contamination could be detected by rechromatography, but this did not preclude distinction being made between the two fractions in terms of DNA structure. NaCl-eluted DNA did not bind to nitrocellulose filters. Caffeine-eluted DNA was retained by the filters and released by washing with 3mM-Tris/HCl,pH9.4. The fractions did not differ in terms of isopycnic centrifugation in CsCl. The NaCl-eluted fraction migrated as a single band in polyacrylamide gels, and this pattern was not modified by prior digestion with Neurospora crassa endonuclease. In contrast, caffeine-eluted DNA contained a minor component having a wide molecular-weight distribution and was subject to limited digestion by the endonuclease. The kinetics of denaturation of NaCi-eluted DNA in the presence of formaldehyde, in common with unfractionated DNA, were consistent with double-stranded structure. The same analysis of caffeine-eluted DNA revealed structural abnormality equivalent to two defects per 10000 base-pairs. The data are consistent with the minor fraction of rat liver DNA, separated by using benzoylated-DEAE-cellulose, containing regions of local denaturation. We previously showed that administration of the hepatocarcinogen dimethylnitrosamine is associated with an increase in the proportion of caffeine-eluted DNA. In terms of most analysis, differences between DNA fraction from nitrosamine-treated rats were similar to differences exhibited by preparations from control animals. However, structural analysis using denaturation kinetics indicated defects in both the NaCl- and caffeine-eluted DNA isolated from nitrosamine-treated rats. The two fractions differed from each other in that caffeine-eluted DNA exhibited a degree of structural damage far greater than that detected in any preparation from control animals..."}
{"id": "PMID:486086", "title": "The reactions of the EcoRi and other restriction endonucleases.", "content": "The reaction of the EcoRI restriction endonuclease was studied with both the plasmid pMB9 and DNA from bacteriophage lambda as the substrates. With both circular and linear DNA molecules, the only reaction catalysed by the EcoRI restriction endonuclease was the hydrolysis of the phosphodiester bond within one strand of the recognition site on the DNA duplex. The cleavage of both strands of the duplex was achieved only after two independent reactions, each involving a single-strand scission. The reactivity of the enzyme for single-strand scissions was the same for both the first and the second cleavage within its recognition site. No differences were observed between the mechanism of action on supercoiled and linear DNA substrates. Other restriction endonucleases were tested against plasmid pMB9. The HindIII restriction endonuclease cleaved DNA in the same manner as the EcoRI enzyme. However, in contrast with EcoRI, the Sa/I and the BamHI restriction endonucleases appeared to cleave both strands of the DNA duplex almost simultaneously. The function of symmetrical DNA sequences and the conformation of the DNA involved in these DNA--protein interactions are discussed in the light of these observations. The fact that the same reactions were observed on both supercoiled and linear DNA substrates implies that these interactions do not involve the unwinding of the duplex before catalysis.", "contents": "The reactions of the EcoRi and other restriction endonucleases. The reaction of the EcoRI restriction endonuclease was studied with both the plasmid pMB9 and DNA from bacteriophage lambda as the substrates. With both circular and linear DNA molecules, the only reaction catalysed by the EcoRI restriction endonuclease was the hydrolysis of the phosphodiester bond within one strand of the recognition site on the DNA duplex. The cleavage of both strands of the duplex was achieved only after two independent reactions, each involving a single-strand scission. The reactivity of the enzyme for single-strand scissions was the same for both the first and the second cleavage within its recognition site. No differences were observed between the mechanism of action on supercoiled and linear DNA substrates. Other restriction endonucleases were tested against plasmid pMB9. The HindIII restriction endonuclease cleaved DNA in the same manner as the EcoRI enzyme. However, in contrast with EcoRI, the Sa/I and the BamHI restriction endonucleases appeared to cleave both strands of the DNA duplex almost simultaneously. The function of symmetrical DNA sequences and the conformation of the DNA involved in these DNA--protein interactions are discussed in the light of these observations. The fact that the same reactions were observed on both supercoiled and linear DNA substrates implies that these interactions do not involve the unwinding of the duplex before catalysis."}
{"id": "PMID:486087", "title": "Complete amino acid sequences of the three collagen-like regions present in subcomponent C1q of the first component of human complement.", "content": "The sequences of amino acid residues 38--51 of the A-chain, and residues 42--90 of the C-chain, of human subcomponent C1q are given. These results, along with previously published sequence data [Reid (1974) Biochem.J. 141, 189--203; Reid (1977) Biochem.J. 161, 247--251; Reid & Thompson (1978) Biochem.J. 173, 863--868] allow the presentation, and comparison with each other, of the complete amino acid sequences of the collagen-like regions found in the A-, B- and C-chains of human subcomponent C1q. Each chain has the continuity of its collagen-like Gly-X-Y repeating triplet amino acid sequence broken. The B- and C-chains have alanine residues at positions B-9 and C-36 where glycine might be expected. The A-chain has a threonine residue at position A-39, which is located between two Gly-X-Y triplets.", "contents": "Complete amino acid sequences of the three collagen-like regions present in subcomponent C1q of the first component of human complement. The sequences of amino acid residues 38--51 of the A-chain, and residues 42--90 of the C-chain, of human subcomponent C1q are given. These results, along with previously published sequence data [Reid (1974) Biochem.J. 141, 189--203; Reid (1977) Biochem.J. 161, 247--251; Reid & Thompson (1978) Biochem.J. 173, 863--868] allow the presentation, and comparison with each other, of the complete amino acid sequences of the collagen-like regions found in the A-, B- and C-chains of human subcomponent C1q. Each chain has the continuity of its collagen-like Gly-X-Y repeating triplet amino acid sequence broken. The B- and C-chains have alanine residues at positions B-9 and C-36 where glycine might be expected. The A-chain has a threonine residue at position A-39, which is located between two Gly-X-Y triplets."}
{"id": "PMID:486088", "title": "The amino acid sequence of ferredoxin from Triticum aestivum (wheat).", "content": "The amino acid sequence of the ferredoxin of Triticum aestivum (wheat) was determined by using a Beckman 890C sequencer in combination with the dansyl--phenylisothiocyanate method to characterize peptides obtained by tryptic, chymotryptic and thermolytic digestion of CNBr-cleavage fragments. The molecule consists of a single polypeptide chain of 97 residues and has an unblocked N-terminus. It shows considerable similarity to other plant-type ferredoxins.", "contents": "The amino acid sequence of ferredoxin from Triticum aestivum (wheat). The amino acid sequence of the ferredoxin of Triticum aestivum (wheat) was determined by using a Beckman 890C sequencer in combination with the dansyl--phenylisothiocyanate method to characterize peptides obtained by tryptic, chymotryptic and thermolytic digestion of CNBr-cleavage fragments. The molecule consists of a single polypeptide chain of 97 residues and has an unblocked N-terminus. It shows considerable similarity to other plant-type ferredoxins."}
{"id": "PMID:486089", "title": "Proteins of the kidney microvillar membrane. The amphipathic form of dipeptidyl peptidase IV.", "content": "Dipeptidyl peptidase IV was solubilized from the microvillar membrane of pig kidney by Triton X-100. The purified enzyme was homogeneous on polyacrylamide-gel electrophoresis and ultracentrifugation, although immunoelectrophoresis indicated that amino-peptidase M was a minor contaminant. A comparison of the detergent-solubilized and proteinase (autolysis)-solubilized forms of the enzyme was undertaken to elucidate the structure and function of the hydrophobic domain that serves to anchor the protein to the membrane. No differences in catalytic properties, nor in sensitivity to inhibition by di-isopropyl phosphorofluoridate were found. On the other hand, several structural differences could be demonstrated. Both forms were about 130,000 subunit mol.wt., but the detergent form appeared to be larger by no more than about 4,000. Electron microscopy showed both forms to be dimers, and gel filtration revealed a difference in the dimeric mol.wt. of about 38 000, mainly attributable to detergent molecules bound to the hydrophobic domain. Papain converted the detergent form into a hydrophilic form that could not be distinguished in properties from the autolysis form. A hydrophobic peptide of about 3500 mol.wt. was identified as a product of papain treatment. The detergent and proteinase forms differed in primary structure. Partial N-terminal amino acid sequences were shown to be different, and the pattern of release of amino acids from the C-terminus by carboxypeptidase Y was essentially similar. The results are consistent with a model in which the protein is anchored to the microvillar membrane by a small hydrophobic domain located within the N-terminal amino acid sequence of the polypeptide chain. The significance of these results in relation to biosynthesis of the enzyme and assembly in the membrane is discussed.", "contents": "Proteins of the kidney microvillar membrane. The amphipathic form of dipeptidyl peptidase IV. Dipeptidyl peptidase IV was solubilized from the microvillar membrane of pig kidney by Triton X-100. The purified enzyme was homogeneous on polyacrylamide-gel electrophoresis and ultracentrifugation, although immunoelectrophoresis indicated that amino-peptidase M was a minor contaminant. A comparison of the detergent-solubilized and proteinase (autolysis)-solubilized forms of the enzyme was undertaken to elucidate the structure and function of the hydrophobic domain that serves to anchor the protein to the membrane. No differences in catalytic properties, nor in sensitivity to inhibition by di-isopropyl phosphorofluoridate were found. On the other hand, several structural differences could be demonstrated. Both forms were about 130,000 subunit mol.wt., but the detergent form appeared to be larger by no more than about 4,000. Electron microscopy showed both forms to be dimers, and gel filtration revealed a difference in the dimeric mol.wt. of about 38 000, mainly attributable to detergent molecules bound to the hydrophobic domain. Papain converted the detergent form into a hydrophilic form that could not be distinguished in properties from the autolysis form. A hydrophobic peptide of about 3500 mol.wt. was identified as a product of papain treatment. The detergent and proteinase forms differed in primary structure. Partial N-terminal amino acid sequences were shown to be different, and the pattern of release of amino acids from the C-terminus by carboxypeptidase Y was essentially similar. The results are consistent with a model in which the protein is anchored to the microvillar membrane by a small hydrophobic domain located within the N-terminal amino acid sequence of the polypeptide chain. The significance of these results in relation to biosynthesis of the enzyme and assembly in the membrane is discussed."}
{"id": "PMID:486090", "title": "Proteins of the kidney microvillar membrane. Immunoelectrophoretic analysis of the membrane hydrolases: identification and resolution of the detergent- and proteinase-solubilized forms.", "content": "Antibodies raised in rabbits to detergent-solubilized pig kidney microvillar proteins have been used to investigate the membrane hydrolases by crossed immunoelectrophoresis. Eight enzymes were detected by specific staining methods: aminopeptidase M, dipeptidylpeptidase IV, neutral endopeptidase, aminopeptidase A, carboxypeptidase P, gamma-glutamyltransferase, trehalase and phosphodiesterase I. The mobility of all these enzymes, with the exception of trehalase and neutral endopeptidase, was increased by treatment of the detergent-solubilized preparation with papain. The difference between the detergent and proteinase forms of these enzymes is attributed to the removal of a small, non-antigenic peptide to which detergent is bound in significant quantities. This interpretation was further supported by experiments in which the microvillus fraction was labelled with an intramembrane photolabelling reagent, 1-azido-4-[125I]iodobenzene. After photolysis, the radioactivity in the membrane could be solubilized by detergent treatment but not by papain treatment. Radioautography after crossed charge-shift immunoelectrophoresis showed a good correlation between charge-shift (signifying the presence of detergent bound to a hydrophobic domain) and the presence of the label.", "contents": "Proteins of the kidney microvillar membrane. Immunoelectrophoretic analysis of the membrane hydrolases: identification and resolution of the detergent- and proteinase-solubilized forms. Antibodies raised in rabbits to detergent-solubilized pig kidney microvillar proteins have been used to investigate the membrane hydrolases by crossed immunoelectrophoresis. Eight enzymes were detected by specific staining methods: aminopeptidase M, dipeptidylpeptidase IV, neutral endopeptidase, aminopeptidase A, carboxypeptidase P, gamma-glutamyltransferase, trehalase and phosphodiesterase I. The mobility of all these enzymes, with the exception of trehalase and neutral endopeptidase, was increased by treatment of the detergent-solubilized preparation with papain. The difference between the detergent and proteinase forms of these enzymes is attributed to the removal of a small, non-antigenic peptide to which detergent is bound in significant quantities. This interpretation was further supported by experiments in which the microvillus fraction was labelled with an intramembrane photolabelling reagent, 1-azido-4-[125I]iodobenzene. After photolysis, the radioactivity in the membrane could be solubilized by detergent treatment but not by papain treatment. Radioautography after crossed charge-shift immunoelectrophoresis showed a good correlation between charge-shift (signifying the presence of detergent bound to a hydrophobic domain) and the presence of the label."}
{"id": "PMID:486091", "title": "The structure and amount of actin in IMR-90 fibroblasts.", "content": "Double-labelling and peptide isolation have been used to examine the homology between the actin of IMR-90 human embryo fibroblasts and muscle actin. After separation of mixtures of [14C]carboxymethylated muscle actin and [3H]carboxymethylated proteins of IMR-90 cells of electrophoresis on sodium dodecyl sulphate-containing polyacrylamide gels, peptides were generated from the material co-migrating with actin by digestion with chymotrypsin. Peptides homologous with peptides accounting for Cys-217, Cys-256, Cys-284 and Cys-373 of muscle actin are present in this material, but no peptide homologous with a Cys-10-containing peptide was detected. From the amount of actin-derived peptides present, the actin content of IMR-90 fibroblasts was calculated to be 4.2% of the total protein of these cells.", "contents": "The structure and amount of actin in IMR-90 fibroblasts. Double-labelling and peptide isolation have been used to examine the homology between the actin of IMR-90 human embryo fibroblasts and muscle actin. After separation of mixtures of [14C]carboxymethylated muscle actin and [3H]carboxymethylated proteins of IMR-90 cells of electrophoresis on sodium dodecyl sulphate-containing polyacrylamide gels, peptides were generated from the material co-migrating with actin by digestion with chymotrypsin. Peptides homologous with peptides accounting for Cys-217, Cys-256, Cys-284 and Cys-373 of muscle actin are present in this material, but no peptide homologous with a Cys-10-containing peptide was detected. From the amount of actin-derived peptides present, the actin content of IMR-90 fibroblasts was calculated to be 4.2% of the total protein of these cells."}
{"id": "PMID:486092", "title": "Immobilized-enzyme pipette. Scope and limitations of a simple device.", "content": "Disposable pipette tips made of polymeric nylon tube with enzymes bound covalently to their inside surface and fixed to the stem of an automatic, adjustable-volume pipette holder together constitutes an immobilized enzyme pipette or 'Impette'. The present paper describes the application in research laboratories and clinics of this new development, with urease as an example in the determination of blood urea.", "contents": "Immobilized-enzyme pipette. Scope and limitations of a simple device. Disposable pipette tips made of polymeric nylon tube with enzymes bound covalently to their inside surface and fixed to the stem of an automatic, adjustable-volume pipette holder together constitutes an immobilized enzyme pipette or 'Impette'. The present paper describes the application in research laboratories and clinics of this new development, with urease as an example in the determination of blood urea."}
{"id": "PMID:486093", "title": "Endocytosis and breakdown of 125I-labelled lactate dehydrogenase isoenzyme M4 by rat liver and spleen in vivo.", "content": "1. Porcine lactate dehydrogenase isoenzyme M4 was labelled with 125I and injected intravenously into rats. Enzyme activity and radioactivity in plasma were cleared in an identical way with a half-life of about 30 min. This half-life was the same as that of unlabelled enzyme. 2. Uptake of label by liver and spleen was determined. Radioactivity in these tissues increased up to about 13 min after injection and subsequently declined. Radioautography indicated uptake of the enzyme by sinusoidal liver cells (probably Kupffer cells) and by spleen macrophages. After differential fractionation of liver, acid-precipitable radioactivity was largely found in the light mitochondrial and microsomal fractions, suggesting localization in lysosomes and endosomes respectively. 3. The amount of acid-soluble radioactive breakdown products in plasma started to rise between 7 and 15 min after injection. Breakdown in liver and spleen was retarded by previous injection of suramin, an inhibitor of lysosomal proteolysis. 4. The contribution of liver and spleen towards the clearance of the enzyme could be calculated from its half-life in plasma and its uptake by the organs within the first 13 min period after injection. Our results indicate that about 65% and 12% of the injected dose was taken up, and subsequently broken down, by liver and spleen respectively. 5. Unlabelled porcine lactate dehydrogenase isoenzyme H4 showed a plasma half-life of about 8 h. This isoenzyme is therefore endocytosed by liver at a much slower rate than isoenzyme M4 (if it is taken up at all).", "contents": "Endocytosis and breakdown of 125I-labelled lactate dehydrogenase isoenzyme M4 by rat liver and spleen in vivo. 1. Porcine lactate dehydrogenase isoenzyme M4 was labelled with 125I and injected intravenously into rats. Enzyme activity and radioactivity in plasma were cleared in an identical way with a half-life of about 30 min. This half-life was the same as that of unlabelled enzyme. 2. Uptake of label by liver and spleen was determined. Radioactivity in these tissues increased up to about 13 min after injection and subsequently declined. Radioautography indicated uptake of the enzyme by sinusoidal liver cells (probably Kupffer cells) and by spleen macrophages. After differential fractionation of liver, acid-precipitable radioactivity was largely found in the light mitochondrial and microsomal fractions, suggesting localization in lysosomes and endosomes respectively. 3. The amount of acid-soluble radioactive breakdown products in plasma started to rise between 7 and 15 min after injection. Breakdown in liver and spleen was retarded by previous injection of suramin, an inhibitor of lysosomal proteolysis. 4. The contribution of liver and spleen towards the clearance of the enzyme could be calculated from its half-life in plasma and its uptake by the organs within the first 13 min period after injection. Our results indicate that about 65% and 12% of the injected dose was taken up, and subsequently broken down, by liver and spleen respectively. 5. Unlabelled porcine lactate dehydrogenase isoenzyme H4 showed a plasma half-life of about 8 h. This isoenzyme is therefore endocytosed by liver at a much slower rate than isoenzyme M4 (if it is taken up at all)."}
{"id": "PMID:486094", "title": "The isolation and partial characterization of the plasma membrane from Trypanosoma brucei.", "content": "Whole sheets of plasma membrane, each with their attached flagellum, were purified from Trypanosoma brucei. The method devised for their isolation included a new technique of cell breakage that used a combination of osmotic stress followed by mechanical sheer and avoided the problem of extreme vesiculation as well as the trapping of organelles in cell 'ghosts'. The purified membranes all contained the pellicular microtubular array. The antigenic surface coat was completely released from the plasma membrane during the isolation procedure. The membranes had a very high cholesterol/phospholipid ratio (1.54). A large proportion (42%) of the cellular DNA was recovered in the plasma-membrane fraction unless a step involving deoxyribonuclease treatment, which decreased the DNA content to less than 13%, was included before secrose-density gradient centrifugation. This step also aided the separation of plasma membranes from other cellular components. The ouabain-sensitive Na+ + K+-stimulated adenosine triphosphatase and adenylate cyclase co-purified with the plasma membranes. Although 5'-nucleotidase was thought to be a plasma-membrane component, it was easily detached from the membrane. The purified membranes were essentially free of L-alanine-alpha-oxoglutarate aminotransferase, L-asparte-alpha-oxoglutarate aminotransferase, malate dehydrogenase, oligomycin-sensitive adenosine triphosphatase, glucose 6-phosphatase, Mg2+-stimulated p-nitrophenyl phosphatase and catalase.", "contents": "The isolation and partial characterization of the plasma membrane from Trypanosoma brucei. Whole sheets of plasma membrane, each with their attached flagellum, were purified from Trypanosoma brucei. The method devised for their isolation included a new technique of cell breakage that used a combination of osmotic stress followed by mechanical sheer and avoided the problem of extreme vesiculation as well as the trapping of organelles in cell 'ghosts'. The purified membranes all contained the pellicular microtubular array. The antigenic surface coat was completely released from the plasma membrane during the isolation procedure. The membranes had a very high cholesterol/phospholipid ratio (1.54). A large proportion (42%) of the cellular DNA was recovered in the plasma-membrane fraction unless a step involving deoxyribonuclease treatment, which decreased the DNA content to less than 13%, was included before secrose-density gradient centrifugation. This step also aided the separation of plasma membranes from other cellular components. The ouabain-sensitive Na+ + K+-stimulated adenosine triphosphatase and adenylate cyclase co-purified with the plasma membranes. Although 5'-nucleotidase was thought to be a plasma-membrane component, it was easily detached from the membrane. The purified membranes were essentially free of L-alanine-alpha-oxoglutarate aminotransferase, L-asparte-alpha-oxoglutarate aminotransferase, malate dehydrogenase, oligomycin-sensitive adenosine triphosphatase, glucose 6-phosphatase, Mg2+-stimulated p-nitrophenyl phosphatase and catalase."}
{"id": "PMID:486095", "title": "Putrescine uptake by the cellular slime mould dictyostelium discoideum.", "content": "1. Rapid labelling occurs when myxamoebae of Dictyostelium discoideum strain AX2 are incubated with [1,4-14C]putrescine. Labelling is energy-dependent. 2. The label enters a pool from which rapid exchange with extracellular putrescine does not occur, and labelling is believed to represent uptake into the cells. 3. The concentration-dependence of putrescine uptake indicates that a number of systems are involved, at least one of which is saturable, with a Km of 9.1 micro M-putrescine. At high putrescine concentrations the overall uptake process is non-saturable. 4. Significant metabolism of putrescine and loss of intracellular putrescine to the medium only occurred when cells were incubated with millimolar concentrations of extracellular putrescine. 5. Putrescine uptake was inhibited by diamines, polyamines, bivalent metal ions and omega-aminocarboxylic acids. 6. The ability to take up putrescine at low concentrations decreased during starvation of myxamoebae. 7. The results are interpreted in terms of a model for putrescine uptake involving adsorptive pinocytosis at low concentrations and fluid-phase pinocytosis at high concentrations.", "contents": "Putrescine uptake by the cellular slime mould dictyostelium discoideum. 1. Rapid labelling occurs when myxamoebae of Dictyostelium discoideum strain AX2 are incubated with [1,4-14C]putrescine. Labelling is energy-dependent. 2. The label enters a pool from which rapid exchange with extracellular putrescine does not occur, and labelling is believed to represent uptake into the cells. 3. The concentration-dependence of putrescine uptake indicates that a number of systems are involved, at least one of which is saturable, with a Km of 9.1 micro M-putrescine. At high putrescine concentrations the overall uptake process is non-saturable. 4. Significant metabolism of putrescine and loss of intracellular putrescine to the medium only occurred when cells were incubated with millimolar concentrations of extracellular putrescine. 5. Putrescine uptake was inhibited by diamines, polyamines, bivalent metal ions and omega-aminocarboxylic acids. 6. The ability to take up putrescine at low concentrations decreased during starvation of myxamoebae. 7. The results are interpreted in terms of a model for putrescine uptake involving adsorptive pinocytosis at low concentrations and fluid-phase pinocytosis at high concentrations."}
{"id": "PMID:486096", "title": "Oxygen-pulse curves in rat liver mitochondrial suspensions. Some observations and deductions.", "content": "1. The inference, implicit in the chemiosmotic hypothesis, that protons move into the bulk phase during ATP synthesis was investigated. 2. Incubation of rat liver mitochondria in the presence of the cation exchanger CM-Sephadex C-50 caused alkalinization in the medium, though total ATP synthesis remained unchanged. The addition of N-ethylmaleimide prevented the alkalinization, but there was still no indication of protons passing into the medium. The expected proton movement [Mitchell & Moyle (1967) Biochem. J. 105, 1147--1162] was readily detected when as an equivalent acid pulse. 3. Analysis of delta H+ decay curves after O2 pulses (3 micrograms-atoms of O/g of protein) indicated the presence of fast and slow components of decay, with first-order rate constants (k) of 0.24s-1 and 0.032s-1. The fast decay was finite and was eliminated in the presence of N-ethylmaleimide. 4. These observations are interpreted as evidence for the development of unmasking of fixed charges on the outer surface of the mitochondrial inner membrane during energization and for the existence of proton-retentive electrical fields (rho-zones) on this surface. The charge concentration is calculated as about 1 charge/10nm2. 5. A cycle of changes in a single fixed-charge molecule is proposed which mediates both Ca2+ uptake and the first step in the utilization of the rho-zone protonmotive force, delta p rho.", "contents": "Oxygen-pulse curves in rat liver mitochondrial suspensions. Some observations and deductions. 1. The inference, implicit in the chemiosmotic hypothesis, that protons move into the bulk phase during ATP synthesis was investigated. 2. Incubation of rat liver mitochondria in the presence of the cation exchanger CM-Sephadex C-50 caused alkalinization in the medium, though total ATP synthesis remained unchanged. The addition of N-ethylmaleimide prevented the alkalinization, but there was still no indication of protons passing into the medium. The expected proton movement [Mitchell & Moyle (1967) Biochem. J. 105, 1147--1162] was readily detected when as an equivalent acid pulse. 3. Analysis of delta H+ decay curves after O2 pulses (3 micrograms-atoms of O/g of protein) indicated the presence of fast and slow components of decay, with first-order rate constants (k) of 0.24s-1 and 0.032s-1. The fast decay was finite and was eliminated in the presence of N-ethylmaleimide. 4. These observations are interpreted as evidence for the development of unmasking of fixed charges on the outer surface of the mitochondrial inner membrane during energization and for the existence of proton-retentive electrical fields (rho-zones) on this surface. The charge concentration is calculated as about 1 charge/10nm2. 5. A cycle of changes in a single fixed-charge molecule is proposed which mediates both Ca2+ uptake and the first step in the utilization of the rho-zone protonmotive force, delta p rho."}
{"id": "PMID:486097", "title": "Bacterial and fungal oxidation of dibenzofuran.", "content": "Cunninghamella elegans and a mutant strain (B8/36) of Beijerinckia both oxidized dibenzofuran to 2,3-dihydroxy-2,3-dihydrodibenzofuran. The bacterial metabolite was extremely unstable and, in the presence of acid, was rapidly converted into a mixture of 2- and 3-hydroxydibenzofuran. In contrast, the 2,3-dihydroxy-2,3-dihydrodibenzofuran formed by C. elegans was stable and only yielded 2- and 3-hydroxydibenzofuran when heated under acidic conditions. The results suggest that Beijerinckia B8/36 and C. elegans form the respective cis- and trans-isomers of 2,3-dihydroxy-2,3-dihydrodibenzofuran. C. elegans also oxidized dibenzofuran to 2- and 3-hydroxydibenzofuran under conditions that would not lead to the dehydration of the trans-dihydrodiol. These observations implicate the initial formation of dibenzofuran- 2,3-epoxide in the fungal oxidation of dibenzofuran. Beijerinckia B8/36 also produced a second unstable dihydrodiol that was tentatively identified as cis-1,2-dihydroxy-1,2-dihydrodibenzofuran. This compound gave 2-hydroxydibenzofuran as the major dehydration product and the cis relative stereochemistry was suggested by the isolation and characterization of an isopropylidine derivative. A preparation of cis-naphthalene dihydrodiol dehydrogenase and cell extracts of the parent strain of Beijerinckia oxidized both bacterial dihydrodiols to catechols. Cell extracts prepared from C. elegans catalysed an analogous oxidation of trans-2,3-dihydroxy-2,3-dihydrodibenzofuran to 2,3-dihydroxydibenzofuran. The latter product was also isolated and identified from culture filtrates. The results suggest that bacteria and fungi utilize different mechanisms to initiate the oxidation of dibenzofuran.", "contents": "Bacterial and fungal oxidation of dibenzofuran. Cunninghamella elegans and a mutant strain (B8/36) of Beijerinckia both oxidized dibenzofuran to 2,3-dihydroxy-2,3-dihydrodibenzofuran. The bacterial metabolite was extremely unstable and, in the presence of acid, was rapidly converted into a mixture of 2- and 3-hydroxydibenzofuran. In contrast, the 2,3-dihydroxy-2,3-dihydrodibenzofuran formed by C. elegans was stable and only yielded 2- and 3-hydroxydibenzofuran when heated under acidic conditions. The results suggest that Beijerinckia B8/36 and C. elegans form the respective cis- and trans-isomers of 2,3-dihydroxy-2,3-dihydrodibenzofuran. C. elegans also oxidized dibenzofuran to 2- and 3-hydroxydibenzofuran under conditions that would not lead to the dehydration of the trans-dihydrodiol. These observations implicate the initial formation of dibenzofuran- 2,3-epoxide in the fungal oxidation of dibenzofuran. Beijerinckia B8/36 also produced a second unstable dihydrodiol that was tentatively identified as cis-1,2-dihydroxy-1,2-dihydrodibenzofuran. This compound gave 2-hydroxydibenzofuran as the major dehydration product and the cis relative stereochemistry was suggested by the isolation and characterization of an isopropylidine derivative. A preparation of cis-naphthalene dihydrodiol dehydrogenase and cell extracts of the parent strain of Beijerinckia oxidized both bacterial dihydrodiols to catechols. Cell extracts prepared from C. elegans catalysed an analogous oxidation of trans-2,3-dihydroxy-2,3-dihydrodibenzofuran to 2,3-dihydroxydibenzofuran. The latter product was also isolated and identified from culture filtrates. The results suggest that bacteria and fungi utilize different mechanisms to initiate the oxidation of dibenzofuran."}
{"id": "PMID:486098", "title": "Heterogeneity and properties of hepatic dexamethasone-binding proteins.", "content": "Evidence from experiments in vivo and in vitro is presented for the presence of three species of dexamethasone-binding proteins in rat liver, which are identified by chromatography on Sepharose 6B or by isoelectric focusing. Although two of these species (DI and DII) possess properties characteristic of a true receptor, the third binding protein (i.e. DIII), which migrates most slowly on Sepharose 6B, but has stability properties similar to protein DII, exhibits a 3-fold lower affinity for dexamethasone and the activated complex neither binds to DNA-cellulose nor translocates to the nucleus. Only the predominant liver receptor (DI), which is eluted first from Sepharose 6B, is present in Novikoff-hepatoma cytosol, suggesting that the major and minor species are not interconverted through simple dissociation during their isolation. The binding activities of all three species in the liver cytosol increase approx. 2-fold in vivo after adrenalectomy and show a transient 2-fold fall in vivo after the administration of cortisol. These changes in vivo in protein DIII shows a marked lag compared with those in proteins DI and DII, which change in parallel. It is therefore proposed that rat liver cytosol contains two dexamethasone receptors and a dexamethasone-binding protein that may be derived from these receptors.", "contents": "Heterogeneity and properties of hepatic dexamethasone-binding proteins. Evidence from experiments in vivo and in vitro is presented for the presence of three species of dexamethasone-binding proteins in rat liver, which are identified by chromatography on Sepharose 6B or by isoelectric focusing. Although two of these species (DI and DII) possess properties characteristic of a true receptor, the third binding protein (i.e. DIII), which migrates most slowly on Sepharose 6B, but has stability properties similar to protein DII, exhibits a 3-fold lower affinity for dexamethasone and the activated complex neither binds to DNA-cellulose nor translocates to the nucleus. Only the predominant liver receptor (DI), which is eluted first from Sepharose 6B, is present in Novikoff-hepatoma cytosol, suggesting that the major and minor species are not interconverted through simple dissociation during their isolation. The binding activities of all three species in the liver cytosol increase approx. 2-fold in vivo after adrenalectomy and show a transient 2-fold fall in vivo after the administration of cortisol. These changes in vivo in protein DIII shows a marked lag compared with those in proteins DI and DII, which change in parallel. It is therefore proposed that rat liver cytosol contains two dexamethasone receptors and a dexamethasone-binding protein that may be derived from these receptors."}
{"id": "PMID:486099", "title": "The presence of two cytochromes b in the facultative methylotroph Pseudomonas AM1.", "content": "Two cytochromes b with absorption maxima at 555 and 562 nm and differing in their mid-point redox potentials are synthesized in Pseudomonas AM1 during growth on methanol or succinate in batch culture, or in NH4+-limited or carbon-limited continuous culture. Both cytochromes b were also present in a cytochrome c-deficient mutant in all growth conditions.", "contents": "The presence of two cytochromes b in the facultative methylotroph Pseudomonas AM1. Two cytochromes b with absorption maxima at 555 and 562 nm and differing in their mid-point redox potentials are synthesized in Pseudomonas AM1 during growth on methanol or succinate in batch culture, or in NH4+-limited or carbon-limited continuous culture. Both cytochromes b were also present in a cytochrome c-deficient mutant in all growth conditions."}
{"id": "PMID:486100", "title": "Studies on the mechanism of experimental porphyria produced by 3,5-diethoxycarbonyl-1,4-dihydrocollidine. Role of a porphyrin-like inhibitor of protohaem ferro-lyase.", "content": "A green porphyrin-like pigment with inhibitory properties towards protohaem ferro-lyase activity was isolated from the livers of mice and rats after treatment with 3,5-diethoxycarbonyl-1,4-dihydrocollidine. Mice, which are more sensitive to the porphyrogenic properties of the drug, produce more inhibitor. The non-porphyrogenic analogue 3,5-diethoxycarbonylcollidine does not cause accumulation of the pigment in the liver. The inhibitory substance is present in control liver at low but measurable concentrations.", "contents": "Studies on the mechanism of experimental porphyria produced by 3,5-diethoxycarbonyl-1,4-dihydrocollidine. Role of a porphyrin-like inhibitor of protohaem ferro-lyase. A green porphyrin-like pigment with inhibitory properties towards protohaem ferro-lyase activity was isolated from the livers of mice and rats after treatment with 3,5-diethoxycarbonyl-1,4-dihydrocollidine. Mice, which are more sensitive to the porphyrogenic properties of the drug, produce more inhibitor. The non-porphyrogenic analogue 3,5-diethoxycarbonylcollidine does not cause accumulation of the pigment in the liver. The inhibitory substance is present in control liver at low but measurable concentrations."}
{"id": "PMID:486101", "title": "Taurine transport in renal brush-border-membrane vesicles.", "content": "Taurine transport in isolated brush-border-membrane vesicles from rat kidney is concentrative and it is driven by the Na+ gradient and transmembrane potential difference; binding is not a significant component of net uptake. The Na+-dependent component of net uptake is saturable with an apparent Km of 17 microM. The taurine-transport mechanism is selective for beta-amino compounds.", "contents": "Taurine transport in renal brush-border-membrane vesicles. Taurine transport in isolated brush-border-membrane vesicles from rat kidney is concentrative and it is driven by the Na+ gradient and transmembrane potential difference; binding is not a significant component of net uptake. The Na+-dependent component of net uptake is saturable with an apparent Km of 17 microM. The taurine-transport mechanism is selective for beta-amino compounds."}
{"id": "PMID:486102", "title": "Antigen- or mitogen-provoked spleen cells produce factors that stimulate the secretion of macrophages of a neutral proteinase degrading cartilage proteoglycans.", "content": "Soluble products released by rabbit spleen cells on stimulation with either mitogen or antigen markedly stimulate the secretion of a proteoglycan-degrading neutral proteinase by rabbit bone-marrow macrophages.", "contents": "Antigen- or mitogen-provoked spleen cells produce factors that stimulate the secretion of macrophages of a neutral proteinase degrading cartilage proteoglycans. Soluble products released by rabbit spleen cells on stimulation with either mitogen or antigen markedly stimulate the secretion of a proteoglycan-degrading neutral proteinase by rabbit bone-marrow macrophages."}
{"id": "PMID:486103", "title": "Effects of protein-deprivation on the regeneration of rat liver after partial hepatectomy.", "content": "Rats maintained on a protein-free diet for 3 days have an altered time course of hepatic DNA synthesis during liver regeneration. The delay in DNA synthesis is eliminated by the administration of casein hydrolysate (given as late as 6h after partial hepatectomy), but not by glucose or incomplete amino acid mixtures. Despite the change in the timing of DNA synthesis, the increases in hepatic amino acid pools, which take place at the earliest stages of the regenerative process, occur in a normal pattern in the regenerating liver of rats fed the protein-free diet. Protein-deprived rats have increased protein synthesis and decreased rates of protein degradation in the liver in response to partial hepatectomy, but these adaptations do not prevent a lag in protein accumulation and low protein/RNA ratios. The regenerating livers of these animals show a deficit in the accumulation of cytoplasmic polyadenylated mRNA as well as a smaller proportion of free polyribosomes. It is suggested that the deficit in free polyribosomes found in the regenerating liver of protein-deprived rats might be a consequence of the slow accumulation of mRNA species coding for intracellular proteins.", "contents": "Effects of protein-deprivation on the regeneration of rat liver after partial hepatectomy. Rats maintained on a protein-free diet for 3 days have an altered time course of hepatic DNA synthesis during liver regeneration. The delay in DNA synthesis is eliminated by the administration of casein hydrolysate (given as late as 6h after partial hepatectomy), but not by glucose or incomplete amino acid mixtures. Despite the change in the timing of DNA synthesis, the increases in hepatic amino acid pools, which take place at the earliest stages of the regenerative process, occur in a normal pattern in the regenerating liver of rats fed the protein-free diet. Protein-deprived rats have increased protein synthesis and decreased rates of protein degradation in the liver in response to partial hepatectomy, but these adaptations do not prevent a lag in protein accumulation and low protein/RNA ratios. The regenerating livers of these animals show a deficit in the accumulation of cytoplasmic polyadenylated mRNA as well as a smaller proportion of free polyribosomes. It is suggested that the deficit in free polyribosomes found in the regenerating liver of protein-deprived rats might be a consequence of the slow accumulation of mRNA species coding for intracellular proteins."}
{"id": "PMID:486104", "title": "A comparison based on delayed light emission and fluorescence induction of intact chloroplasts isolated from mesophyll protoplasts and bundle-sheath cells of maize.", "content": "Chloroplasts or cells from maize (Zea mays) bundle sheath show a very low intensity of delayed light emission compared with mesophyll protoplasts or chloroplasts. The bundle-sheath chloroplasts retain only the fast (less than 1 ms) component of the emission. They also fail to show fluorescence induction in contrast to the mesophyll, which behaved normally. The mesophyll material could be made to resemble the bundle-sheath chloroplasts in respect of both phenomena by adding to it 1-(3',4'-dichlorophenyl)-3,3-dimethyl-urea and hydroxylamine, together. It is concluded that Photosystem II, that gives rise to both effects, is not active in the bundle sheath but may be present in an inhibited form.", "contents": "A comparison based on delayed light emission and fluorescence induction of intact chloroplasts isolated from mesophyll protoplasts and bundle-sheath cells of maize. Chloroplasts or cells from maize (Zea mays) bundle sheath show a very low intensity of delayed light emission compared with mesophyll protoplasts or chloroplasts. The bundle-sheath chloroplasts retain only the fast (less than 1 ms) component of the emission. They also fail to show fluorescence induction in contrast to the mesophyll, which behaved normally. The mesophyll material could be made to resemble the bundle-sheath chloroplasts in respect of both phenomena by adding to it 1-(3',4'-dichlorophenyl)-3,3-dimethyl-urea and hydroxylamine, together. It is concluded that Photosystem II, that gives rise to both effects, is not active in the bundle sheath but may be present in an inhibited form."}
{"id": "PMID:486105", "title": "Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.", "content": "A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.", "contents": "Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport. A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte."}
{"id": "PMID:486106", "title": "Selective stimulation by tri-iodothyronine of myocardial deoxyribonucleic acid polymerase-alpha in neonatal rats.", "content": "Three forms of DNA polymerase (alpha, beta and gamma) were separated from isolated rat myocardial cells on the basis of template, pH and ionic requirements, sensitivity to N-ethylmaleimide and position on sucrose gradients. Tri-iodothyronine administration (20mug/100g intraperitoneally) to 3-week-old rats resulted in selective stimulation of DNA polymerase-alpha (198+/-7.1 versus 102+/-5.8pmol of [(3)H]dTMP/30min per mg of protein in untreated controls, P<0.01), with no change in polymerases-beta and -gamma. [(3)H]Thymidine incorporation into myocardial DNA was also enhanced in tri-iodothyronine-treated neonatal rats (132+/-11.2 versus 53+/-4.1c.p.m./mug of DNA in controls, P<0.001). Increased incorporation was associated with an expansion of deoxyribonucleoside 5'-triphosphate pools, especially that of dTTP (24+/-1.6 versus 10+/-1.1pmol/mg of DNA, P<0.01). Neither DNA polymerase activities nor [(3)H]thymidine incorporation were changed in 6-month-old rats in response to tri-iodothyronine. Unstimulated adult myocardial cells had DNA polymerase activities comparable with those in 3-week-old animals, but significantly lower [(3)H]-thymidine incorporation and deoxyribonucleoside triphosphate concentrations. Enhancement of both DNA polymerase-alpha activity and [(3)H]thymidine incorporation in tri-iodothyronine-treated young rats was prevented by concomitant administration of either vinblastine (1mug/g) or daunomycin (2mug/g); actinomycin D (0.1mug/g) or cycloheximide (8mug/g), on the other hand, prevented the increase in [(3)H]thymidine incorporation, but not DNA polymerase-alpha activation. These results demonstrate an age-dependent stimulation of myocardial DNA replication by tri-iodothyronine and suggest an inter-relationship between DNA synthesis and subsequent entry into mitosis.", "contents": "Selective stimulation by tri-iodothyronine of myocardial deoxyribonucleic acid polymerase-alpha in neonatal rats. Three forms of DNA polymerase (alpha, beta and gamma) were separated from isolated rat myocardial cells on the basis of template, pH and ionic requirements, sensitivity to N-ethylmaleimide and position on sucrose gradients. Tri-iodothyronine administration (20mug/100g intraperitoneally) to 3-week-old rats resulted in selective stimulation of DNA polymerase-alpha (198+/-7.1 versus 102+/-5.8pmol of [(3)H]dTMP/30min per mg of protein in untreated controls, P<0.01), with no change in polymerases-beta and -gamma. [(3)H]Thymidine incorporation into myocardial DNA was also enhanced in tri-iodothyronine-treated neonatal rats (132+/-11.2 versus 53+/-4.1c.p.m./mug of DNA in controls, P<0.001). Increased incorporation was associated with an expansion of deoxyribonucleoside 5'-triphosphate pools, especially that of dTTP (24+/-1.6 versus 10+/-1.1pmol/mg of DNA, P<0.01). Neither DNA polymerase activities nor [(3)H]thymidine incorporation were changed in 6-month-old rats in response to tri-iodothyronine. Unstimulated adult myocardial cells had DNA polymerase activities comparable with those in 3-week-old animals, but significantly lower [(3)H]-thymidine incorporation and deoxyribonucleoside triphosphate concentrations. Enhancement of both DNA polymerase-alpha activity and [(3)H]thymidine incorporation in tri-iodothyronine-treated young rats was prevented by concomitant administration of either vinblastine (1mug/g) or daunomycin (2mug/g); actinomycin D (0.1mug/g) or cycloheximide (8mug/g), on the other hand, prevented the increase in [(3)H]thymidine incorporation, but not DNA polymerase-alpha activation. These results demonstrate an age-dependent stimulation of myocardial DNA replication by tri-iodothyronine and suggest an inter-relationship between DNA synthesis and subsequent entry into mitosis."}
{"id": "PMID:486107", "title": "Interaction of lipophilic quinones with membrane fragments of Paracoccus denitrificans and Staphylococcus epidermidis.", "content": "In quinone-depleted mitochondrial and Paracoccus denitrificans membranes the quantum yield of fluorescence of ostruthin (6-geranyl-7-hydroxycoumarin) was maintained, whereas an increase in the quantum yield took place after extraction of Staphylococcus epidermidis membrane. A marked quenching effect of ubiquinone and menaquinone each with two isoprene units in the side chain on the ostruthin fluorescence was found with all types of quinone-depleted particles. When the homogues of menaquinone and ubiquinone with six isoprene units in the side chain were re-incorporated, a quenching of the ostruthin fluorescence was observed in the S. epidermidis membranes but not in those of P. denitrificans. The different behaviour of both bacterial preparations is attributable to the more specific finding of ubiquinone in the particles of P. denitrificans.", "contents": "Interaction of lipophilic quinones with membrane fragments of Paracoccus denitrificans and Staphylococcus epidermidis. In quinone-depleted mitochondrial and Paracoccus denitrificans membranes the quantum yield of fluorescence of ostruthin (6-geranyl-7-hydroxycoumarin) was maintained, whereas an increase in the quantum yield took place after extraction of Staphylococcus epidermidis membrane. A marked quenching effect of ubiquinone and menaquinone each with two isoprene units in the side chain on the ostruthin fluorescence was found with all types of quinone-depleted particles. When the homogues of menaquinone and ubiquinone with six isoprene units in the side chain were re-incorporated, a quenching of the ostruthin fluorescence was observed in the S. epidermidis membranes but not in those of P. denitrificans. The different behaviour of both bacterial preparations is attributable to the more specific finding of ubiquinone in the particles of P. denitrificans."}
{"id": "PMID:486108", "title": "Effects of diamines on ornithine decarboxylase activity in control and virally transformed mouse fibroblasts.", "content": "1. The induction of ornithine decarboxylase activity in mouse 3T3 fibroblasts or an SV-40 transformed 3T3 cell line by serum was prevented by addition of the naturally occurring polyamines putrescine (butane-1,4-diamine) and spermidine. Much higher concentrations of these amines were required to fully suppress ornithine decarboxylase activity in the transformed SV-3T3 cells than in the 3T3 fibroblasts. 2. Synthetic alpha omega-diamines with 3--12 carbon atoms also prevented the increase in ornithine decarboxylase activity induced by serum in these cells. The longer chain diamines were somewhat more potent than propane-1,3-diamine in this effect, but the synthetic diamines were less active than putrescine in the 3T3 cells. There was little difference between the responses of 3T3 and SV-3T3 cells to the synthetic diamines propane-1,3-diamine and heptane-1,7-diamine. 3. These results are discussed in relation to the control of polyamine synthesis in mammalian cells.", "contents": "Effects of diamines on ornithine decarboxylase activity in control and virally transformed mouse fibroblasts. 1. The induction of ornithine decarboxylase activity in mouse 3T3 fibroblasts or an SV-40 transformed 3T3 cell line by serum was prevented by addition of the naturally occurring polyamines putrescine (butane-1,4-diamine) and spermidine. Much higher concentrations of these amines were required to fully suppress ornithine decarboxylase activity in the transformed SV-3T3 cells than in the 3T3 fibroblasts. 2. Synthetic alpha omega-diamines with 3--12 carbon atoms also prevented the increase in ornithine decarboxylase activity induced by serum in these cells. The longer chain diamines were somewhat more potent than propane-1,3-diamine in this effect, but the synthetic diamines were less active than putrescine in the 3T3 cells. There was little difference between the responses of 3T3 and SV-3T3 cells to the synthetic diamines propane-1,3-diamine and heptane-1,7-diamine. 3. These results are discussed in relation to the control of polyamine synthesis in mammalian cells."}
{"id": "PMID:486109", "title": "Induction of liver cell haem oxygenase in iron-overloaded rats.", "content": "Rats were chronically iron-overloaded by intraperitonel injections of iron-dextran. Electron microscopy revealed that the excess iron was deposited in ferritin-like particles packed in lysosomes and scattered in hepatic cytoplasm. No mitochondrial iron deposition or damage was seen. Furthermore, mitochondrial preparations from chronically iron-overloaded animals were found to be contaminated with lysosomes, which could explain previously reported increases in mitochondrial iron by chemical analysis. Mitochondrial function, as measured by cytochromes a-a3, b and c concentrations as well as activity of the rate-limiting enzyme of haem synthesis, delta-aminolaevulinate synthetase, was not diminished by chronic iron-overloading. Microsomal haem was decreased by 30% at the time that haem oxygenase, the rate-limiting enzyme of haem degradation, was increased approx. 3-fold. Animals were given a single intraperitoneal injection of iron-dextran and the activities of delta-aminolaevulinate synthetase and haem oxygenase were measured over 24 h. delta-Aminolaevulinate synthetase activity increased approx. 2-fold in these acutely iron-overloaded rat livers, but at a time after the increase in haem oxygenase. These results suggest that an early consequence of excess iron in liver is acceleration of the rate of haem degradation, possible by haem oxygenase.", "contents": "Induction of liver cell haem oxygenase in iron-overloaded rats. Rats were chronically iron-overloaded by intraperitonel injections of iron-dextran. Electron microscopy revealed that the excess iron was deposited in ferritin-like particles packed in lysosomes and scattered in hepatic cytoplasm. No mitochondrial iron deposition or damage was seen. Furthermore, mitochondrial preparations from chronically iron-overloaded animals were found to be contaminated with lysosomes, which could explain previously reported increases in mitochondrial iron by chemical analysis. Mitochondrial function, as measured by cytochromes a-a3, b and c concentrations as well as activity of the rate-limiting enzyme of haem synthesis, delta-aminolaevulinate synthetase, was not diminished by chronic iron-overloading. Microsomal haem was decreased by 30% at the time that haem oxygenase, the rate-limiting enzyme of haem degradation, was increased approx. 3-fold. Animals were given a single intraperitoneal injection of iron-dextran and the activities of delta-aminolaevulinate synthetase and haem oxygenase were measured over 24 h. delta-Aminolaevulinate synthetase activity increased approx. 2-fold in these acutely iron-overloaded rat livers, but at a time after the increase in haem oxygenase. These results suggest that an early consequence of excess iron in liver is acceleration of the rate of haem degradation, possible by haem oxygenase."}
{"id": "PMID:486110", "title": "Differences between lactating and non-lactating dairy cows in concentration and secretion rate of insulin.", "content": "1. Four parameters of insulin metabolism were compared in catheterized lactating and non-lactating Friesian x Ayrshire dairy cows. 2. The four parameters, i.e. arterial and portal-venous concentrations of insulin, and pancreatic output and hepatic uptake of insulin, were approx. 2-, 3-, 3-  and 5-fold higher respectively in the non-lactating cows than in the lactating cows in the normal fed state. Statistical significance was not achieved for the differences in magnitude in the case of the latter two parameters, however. 3. All four parameters increased significantly about 4-fold when non-lactating cows were infused intravenously with glucose for 48 h at a rate of 4.2 mmol/min. The parameters also increased in the lactating cows during glucose infusion, but the values reached were substantially lower than in the non-lactating cows and the increases were not statistically significant. 4. Arterial insulin concentrations doubled in the non-lactating cows during a 3 h infusion of propionate into a mesenteric vein, but remained unaltered in the lactating cows. 5. Differences in insulin concentration and output between the lactating and non-lactating cows were not consistently related to differences in either glucose concentration or glucose-entry rate. Arterial propionate concentrations were similar in both groups of cows at all times. 6. It is concluded that in the dairy cow, insulin secretion in response to an insulinotropic agent is diminished during lactation.", "contents": "Differences between lactating and non-lactating dairy cows in concentration and secretion rate of insulin. 1. Four parameters of insulin metabolism were compared in catheterized lactating and non-lactating Friesian x Ayrshire dairy cows. 2. The four parameters, i.e. arterial and portal-venous concentrations of insulin, and pancreatic output and hepatic uptake of insulin, were approx. 2-, 3-, 3-  and 5-fold higher respectively in the non-lactating cows than in the lactating cows in the normal fed state. Statistical significance was not achieved for the differences in magnitude in the case of the latter two parameters, however. 3. All four parameters increased significantly about 4-fold when non-lactating cows were infused intravenously with glucose for 48 h at a rate of 4.2 mmol/min. The parameters also increased in the lactating cows during glucose infusion, but the values reached were substantially lower than in the non-lactating cows and the increases were not statistically significant. 4. Arterial insulin concentrations doubled in the non-lactating cows during a 3 h infusion of propionate into a mesenteric vein, but remained unaltered in the lactating cows. 5. Differences in insulin concentration and output between the lactating and non-lactating cows were not consistently related to differences in either glucose concentration or glucose-entry rate. Arterial propionate concentrations were similar in both groups of cows at all times. 6. It is concluded that in the dairy cow, insulin secretion in response to an insulinotropic agent is diminished during lactation."}
{"id": "PMID:486111", "title": "Interaction between glucocorticoids and glucagon in the hormonal modification of calcium retention by isolated rat liver mitochondria.", "content": "1. The administration of dexamethasone to intact fed rats by intraperitoneal injection for 3h was associated with a 6-fold increase in the time for which mitochondria subsequently isolated from the liver retain a given load of exogenous Ca2+. This effect was blocked by the co-administration of cycloheximide with dexamethasone, and partially blocked by the co-administration of puromycin. Daily administration of dexamethasone for periods of 4--7 days resulted in liver mitochondria that exhibited a decreased ability to retain exogenous Ca2+. 2. When glucagon was administered to fed adrenalectomized rats, the increase in mitochondrial Ca2+-retention time that results from the action of this hormone was reduced by 50% when compared with its effect on intact animals. The administration of dexamethasone to adrenalectomized rats partially restored the full effect of glucagon. 3. Dexamethasone did not enhance the effect of glucagon on mitochondrial Ca2+-retention time when administered to intact fed rats. 4. It is concluded that these data support the hypothesis that the hormone-induced modification of liver mitochondria, which results in an increase in the time for which exogenous Ca2+ is retained, involves a step in which new protein is synthesized.", "contents": "Interaction between glucocorticoids and glucagon in the hormonal modification of calcium retention by isolated rat liver mitochondria. 1. The administration of dexamethasone to intact fed rats by intraperitoneal injection for 3h was associated with a 6-fold increase in the time for which mitochondria subsequently isolated from the liver retain a given load of exogenous Ca2+. This effect was blocked by the co-administration of cycloheximide with dexamethasone, and partially blocked by the co-administration of puromycin. Daily administration of dexamethasone for periods of 4--7 days resulted in liver mitochondria that exhibited a decreased ability to retain exogenous Ca2+. 2. When glucagon was administered to fed adrenalectomized rats, the increase in mitochondrial Ca2+-retention time that results from the action of this hormone was reduced by 50% when compared with its effect on intact animals. The administration of dexamethasone to adrenalectomized rats partially restored the full effect of glucagon. 3. Dexamethasone did not enhance the effect of glucagon on mitochondrial Ca2+-retention time when administered to intact fed rats. 4. It is concluded that these data support the hypothesis that the hormone-induced modification of liver mitochondria, which results in an increase in the time for which exogenous Ca2+ is retained, involves a step in which new protein is synthesized."}
{"id": "PMID:486112", "title": "Macrophage protein turnover. Evidence for lysosomal participation in basal proteolysis.", "content": "1. Turnover of intracellular proteins in cultured mouse macrophages was found to be slightly accelerated by the omission of serum from the culture medium. Media containing 10% (v/v) or more of serum established basal degradation rates in the cultures. 2. Basal degradation rates varied considerably between experiments, probably as a result of variable activation in vivo of the macrophages. 3. The selective carboxyl proteinase inhibitor pepstatin, which appeared to enter the lysosomes of the cells by pinocytosis, gave a progressive inhibition of basal proteolysis up to a maximum of about 40%. Cellular cathepsin D was largely inhibited after 48h of cultivation with pepstatin (100 micrograms/ml). 4. Leupeptin and 7-amino-1-chloro-3-tosylamidoheptan-2-one are less selective proteinase inhibitors. They also induced 25--35% inhibition of degradation, but their actions may not have been restricted to lysosomes. 5. Several solutes and particles that are endocytosed by macrophages and stored in lysosomes induce some inhibition of basal proteolysis, whether or not they themselves are substrates for proteolysis. 6. Colchicine was without effect on protein degradation, but cytochalasin B and the local anesthetics lidocaine and procaine, all of which have effects on microfilaments, were significantly inhibitory. This inhibition may result from a decrease in the rate of autophagy, and thus of lysosomal proteolysis, due to prevention of microfilament action.", "contents": "Macrophage protein turnover. Evidence for lysosomal participation in basal proteolysis. 1. Turnover of intracellular proteins in cultured mouse macrophages was found to be slightly accelerated by the omission of serum from the culture medium. Media containing 10% (v/v) or more of serum established basal degradation rates in the cultures. 2. Basal degradation rates varied considerably between experiments, probably as a result of variable activation in vivo of the macrophages. 3. The selective carboxyl proteinase inhibitor pepstatin, which appeared to enter the lysosomes of the cells by pinocytosis, gave a progressive inhibition of basal proteolysis up to a maximum of about 40%. Cellular cathepsin D was largely inhibited after 48h of cultivation with pepstatin (100 micrograms/ml). 4. Leupeptin and 7-amino-1-chloro-3-tosylamidoheptan-2-one are less selective proteinase inhibitors. They also induced 25--35% inhibition of degradation, but their actions may not have been restricted to lysosomes. 5. Several solutes and particles that are endocytosed by macrophages and stored in lysosomes induce some inhibition of basal proteolysis, whether or not they themselves are substrates for proteolysis. 6. Colchicine was without effect on protein degradation, but cytochalasin B and the local anesthetics lidocaine and procaine, all of which have effects on microfilaments, were significantly inhibitory. This inhibition may result from a decrease in the rate of autophagy, and thus of lysosomal proteolysis, due to prevention of microfilament action."}
{"id": "PMID:486113", "title": "A high-affinity oestrogen-binding protein in cockerel liver cytosol.", "content": "In contrast with several earlier reports, cytosol from cockerel liver contains a significant concentration of a protein that binds oestradiol with high affinity. To demonstrate the activity, certain alterations in the conventional method of preparation of cytosol must be made. Homogenization in sucrose-containing buffer at pH 8.4 in the presence of proteinase inhibitors and rapid fractionation of the cytosol with (NH4)2SO4 enables demonstration of a single class of oestradiol-binding sites with a Kd of about 1 nM and specificity only for oestrogens. The concentration is about 300 sites per cell in liver from 2-week-old cockerels. Oestradiol treatment in vivo decreases the number of exchangeable cytosol oestradiol-binding sites by about 80% for 1--4h, after which time it is gradually restored. Gel filtration of the cytosol preparation in the presence of high salt concentrations reveals that most of the oestradiol-binding activity is in high-molecular-weight aggregates, but a mild trypsin treatment generates a specific binding protein with an approximate mol.wt. of 40 000. This protein may be an oestrogen receptor.", "contents": "A high-affinity oestrogen-binding protein in cockerel liver cytosol. In contrast with several earlier reports, cytosol from cockerel liver contains a significant concentration of a protein that binds oestradiol with high affinity. To demonstrate the activity, certain alterations in the conventional method of preparation of cytosol must be made. Homogenization in sucrose-containing buffer at pH 8.4 in the presence of proteinase inhibitors and rapid fractionation of the cytosol with (NH4)2SO4 enables demonstration of a single class of oestradiol-binding sites with a Kd of about 1 nM and specificity only for oestrogens. The concentration is about 300 sites per cell in liver from 2-week-old cockerels. Oestradiol treatment in vivo decreases the number of exchangeable cytosol oestradiol-binding sites by about 80% for 1--4h, after which time it is gradually restored. Gel filtration of the cytosol preparation in the presence of high salt concentrations reveals that most of the oestradiol-binding activity is in high-molecular-weight aggregates, but a mild trypsin treatment generates a specific binding protein with an approximate mol.wt. of 40 000. This protein may be an oestrogen receptor."}
{"id": "PMID:486114", "title": "Sensitivity of glucose uptake and lipolysis of white adipocytes of the rat to insulin and effects of some metabolites.", "content": "1. Insulin increased glucose uptake and inhibited lipolysis in white adipocytes of the rat over the same concentration range of the hormone: the half-maximal effects were observed at approx. 10 microunits of insulin/ml. Thus, contrary to previous reports, no difference in sensitivity of the two processes to insulin could be found, which suggests that both these effects of insulin are important in increasing the rate of glucose utilization after a meal. 2. Adenosine deaminase, which lowers the concentration of adenosine in the incubation medium, decreased the sensitivity of both processes (lipolysis and glucose uptake) to insulin: this suggests that adenosine increases the sensitivity of both processes. Similarly, lactate and 3-hydroxybutyrate increased the sensitivity of both processes (to the same extent) to insulin. It is suggested that this increased sensitivity will improve the response (of adipose tissue) to insulin on refeeding after a prolonged period of starvation (when the hydroxybutyrate concentration is high), and after a short burst of exercise, when the blood lactate concentration is high and when large amounts of glucose are produced from lactate via gluconeogenesis in the liver.", "contents": "Sensitivity of glucose uptake and lipolysis of white adipocytes of the rat to insulin and effects of some metabolites. 1. Insulin increased glucose uptake and inhibited lipolysis in white adipocytes of the rat over the same concentration range of the hormone: the half-maximal effects were observed at approx. 10 microunits of insulin/ml. Thus, contrary to previous reports, no difference in sensitivity of the two processes to insulin could be found, which suggests that both these effects of insulin are important in increasing the rate of glucose utilization after a meal. 2. Adenosine deaminase, which lowers the concentration of adenosine in the incubation medium, decreased the sensitivity of both processes (lipolysis and glucose uptake) to insulin: this suggests that adenosine increases the sensitivity of both processes. Similarly, lactate and 3-hydroxybutyrate increased the sensitivity of both processes (to the same extent) to insulin. It is suggested that this increased sensitivity will improve the response (of adipose tissue) to insulin on refeeding after a prolonged period of starvation (when the hydroxybutyrate concentration is high), and after a short burst of exercise, when the blood lactate concentration is high and when large amounts of glucose are produced from lactate via gluconeogenesis in the liver."}
{"id": "PMID:486115", "title": "Interaction between membrane functions and protein synthesis in reticulocytes. An elongation-stage inhibitor of protein synthesis extracted from the reticulocyte membrane.", "content": "A component of the reticulocyte cell membrane was found to inhibit protein synthesis severely in a reticulocyte lysate system. An investigation into the mode of action of the membrane inhibitor revealed the following facts. (1) The binding of the tertiary initiation complex (methionyl-tRNAfMet-Initiation Factor 2-GTP) to the 40S ribosomal subunit was unaffected by the membrane inhibitor. (2) The membrane component did not interfere with the binding of the 40S initiation complex to the AUG initiation codon and subsequent attachment of the 60S ribosomal subunit. (3) Elongation of the peptide chain, as assayed by peptidyl-puromycin formation, was markedly affected by the membrane inhibitor. Surprisingly, the membrane component caused a considerable increase in peptidyl-puromycin formation. (4) Reticulocyte ribosomes that had been reisolated by high-speed centrifugation, after preincubation with the membrane component, were found to be highly defective when assayed in a cell-free protein-synthesizing system. These results indicated that an extract of the reticulocyte cell membrane inhibited protein synthesis by interacting with the ribosome and thus interfered with the correct functions of the elongation stage of protein synthesis. The implications of this conclusion are discussed in the light of data showing that a highly purified preparation of the membrane inhibitor also displayed an endonucleolytic activity highly specific for 28S RNA.", "contents": "Interaction between membrane functions and protein synthesis in reticulocytes. An elongation-stage inhibitor of protein synthesis extracted from the reticulocyte membrane. A component of the reticulocyte cell membrane was found to inhibit protein synthesis severely in a reticulocyte lysate system. An investigation into the mode of action of the membrane inhibitor revealed the following facts. (1) The binding of the tertiary initiation complex (methionyl-tRNAfMet-Initiation Factor 2-GTP) to the 40S ribosomal subunit was unaffected by the membrane inhibitor. (2) The membrane component did not interfere with the binding of the 40S initiation complex to the AUG initiation codon and subsequent attachment of the 60S ribosomal subunit. (3) Elongation of the peptide chain, as assayed by peptidyl-puromycin formation, was markedly affected by the membrane inhibitor. Surprisingly, the membrane component caused a considerable increase in peptidyl-puromycin formation. (4) Reticulocyte ribosomes that had been reisolated by high-speed centrifugation, after preincubation with the membrane component, were found to be highly defective when assayed in a cell-free protein-synthesizing system. These results indicated that an extract of the reticulocyte cell membrane inhibited protein synthesis by interacting with the ribosome and thus interfered with the correct functions of the elongation stage of protein synthesis. The implications of this conclusion are discussed in the light of data showing that a highly purified preparation of the membrane inhibitor also displayed an endonucleolytic activity highly specific for 28S RNA."}
{"id": "PMID:486116", "title": "A tissue-culture model of cartilage breakdown in rheumatoid arthritis. Quantitative aspects of proteoglycan release.", "content": "1. The destruction of articular cartilage in human rheumatoid and other arthritides is the result of diverse mechanical, inflammatory and local cellular factors. A tissue-culture model for studying cartilage-synovial interactions that may be involved in the final common pathway of joint destruction is described. 2. Matrix breakdown was studied in vitro by using bovine nasal-cartilage discs cultivated in contact with synovium. Synovia were obtained from human and animal sources. Human tissue came from patients with ;classical' rheumatoid arthritis, and animal tissue from rabbits with antigen-induced arthritis. 3. Cartilage discs increased their proteoglycan content 2-3-fold during 8 days in culture. Proteoglycan was also released into culture medium, approx. 70% arising from cartilage breakdown. 4. Synovial explants from human rheumatoid and rabbit antigen-induced arthritis produced equivalent stimulation of proteoglycan release. After an initial lag phase, the breakdown rate rose abruptly to a maximum, resulting in a 2-fold increase of proteoglycan accumulation in culture medium after 8-10 days. 5. High-molecular-weight products shed into culture media were characterized chromatographically and by differential enzymic digestion. Proteoglycan-chondroitin sulphate accounted for 90% of the released polyanion, and its partial degradation in the presence of synovial explants was consistent with limited proteolytic cleavage. 6. Rheumatoid synovium applied to dead cartilage increased the basal rate of proteoglycan release. Living cartilage was capable of more extensive autolysis, even in the absence of synovium. However, optimal proteoglycan release required the interaction of living synovium with live cartilage. These findings support the view that a significant component of cartilage breakdown may be chondrocyte-mediated.", "contents": "A tissue-culture model of cartilage breakdown in rheumatoid arthritis. Quantitative aspects of proteoglycan release. 1. The destruction of articular cartilage in human rheumatoid and other arthritides is the result of diverse mechanical, inflammatory and local cellular factors. A tissue-culture model for studying cartilage-synovial interactions that may be involved in the final common pathway of joint destruction is described. 2. Matrix breakdown was studied in vitro by using bovine nasal-cartilage discs cultivated in contact with synovium. Synovia were obtained from human and animal sources. Human tissue came from patients with ;classical' rheumatoid arthritis, and animal tissue from rabbits with antigen-induced arthritis. 3. Cartilage discs increased their proteoglycan content 2-3-fold during 8 days in culture. Proteoglycan was also released into culture medium, approx. 70% arising from cartilage breakdown. 4. Synovial explants from human rheumatoid and rabbit antigen-induced arthritis produced equivalent stimulation of proteoglycan release. After an initial lag phase, the breakdown rate rose abruptly to a maximum, resulting in a 2-fold increase of proteoglycan accumulation in culture medium after 8-10 days. 5. High-molecular-weight products shed into culture media were characterized chromatographically and by differential enzymic digestion. Proteoglycan-chondroitin sulphate accounted for 90% of the released polyanion, and its partial degradation in the presence of synovial explants was consistent with limited proteolytic cleavage. 6. Rheumatoid synovium applied to dead cartilage increased the basal rate of proteoglycan release. Living cartilage was capable of more extensive autolysis, even in the absence of synovium. However, optimal proteoglycan release required the interaction of living synovium with live cartilage. These findings support the view that a significant component of cartilage breakdown may be chondrocyte-mediated."}
{"id": "PMID:486117", "title": "Lower rates of protein degradation in developing rat brain.", "content": "Protein-degradation rates in developing rat brain were estimated from the decay in total radioactivity in proteins labelled by a single intraperitoneal injection of NaH14CO3 to 5-day-old animals. In contrast with previous reports, our results indicate that degradation rates are lower in developing than in adult brain and suggest that in brain, as has been observed in liver, adrenal gland, muscle, cultured mammalian cells and bacteria, reduced rates of protein degradation contribute to the increase in protein content under conditions of rapid growth.", "contents": "Lower rates of protein degradation in developing rat brain. Protein-degradation rates in developing rat brain were estimated from the decay in total radioactivity in proteins labelled by a single intraperitoneal injection of NaH14CO3 to 5-day-old animals. In contrast with previous reports, our results indicate that degradation rates are lower in developing than in adult brain and suggest that in brain, as has been observed in liver, adrenal gland, muscle, cultured mammalian cells and bacteria, reduced rates of protein degradation contribute to the increase in protein content under conditions of rapid growth."}
{"id": "PMID:486118", "title": "The effect of surgical trauma on muscle protein turnover in rats. A serious methodological misunderstanding.", "content": "The reported rates of protein degradation in a recent paper on the effect of surgical trauma on muscle protein turnover [Hoover-Plow & Clifford (1978) Biochem. J. 176, 137--142] have no real meaning because of a serious methodological misunderstanding by the authors. In addition, there are problems involved in the determination of synthesis rates, so that the reported effects of trauma on muscle protein turnover can be discounted.", "contents": "The effect of surgical trauma on muscle protein turnover in rats. A serious methodological misunderstanding. The reported rates of protein degradation in a recent paper on the effect of surgical trauma on muscle protein turnover [Hoover-Plow & Clifford (1978) Biochem. J. 176, 137--142] have no real meaning because of a serious methodological misunderstanding by the authors. In addition, there are problems involved in the determination of synthesis rates, so that the reported effects of trauma on muscle protein turnover can be discounted."}
{"id": "PMID:486119", "title": "Adrenaline responsiveness of glucose metabolism in insulin-resistant adipose tissue of rats fed a high-fat diet.", "content": "The effects of adrenaline (0.5 microM) and the combination of adrenaline and insulin (1.7nM) on [6-14C]glucose metabolism were assessed in epididymal fat-pads from rats fed either a low- or high-fat diet. The response of lipolysis to adrenaline was clearly diminished in fat-fed rats. Insulin added to adrenaline inhibited the lipolysis by 50% regardless of the diet. Glucose utilization in adipose tissue of fat-fed rats was markedly stimulated by adrenaline (glucose uptake was increased 3-fold and the production of CO2 and the glycerol moiety of acylglycerol was increased 4-fold). However, adipose tissue from fat-fed rats was resistant to the effect of insulin to produce a further increase in adrenaline-stimulated glucose uptake. The intracellular capacity of lipogenesis on the one hand, and the production of CO2 and the glycerol moiety of acylglycerol on the other, are of prime importance in the action of insulin and adrenaline on glucose utilization in this model.", "contents": "Adrenaline responsiveness of glucose metabolism in insulin-resistant adipose tissue of rats fed a high-fat diet. The effects of adrenaline (0.5 microM) and the combination of adrenaline and insulin (1.7nM) on [6-14C]glucose metabolism were assessed in epididymal fat-pads from rats fed either a low- or high-fat diet. The response of lipolysis to adrenaline was clearly diminished in fat-fed rats. Insulin added to adrenaline inhibited the lipolysis by 50% regardless of the diet. Glucose utilization in adipose tissue of fat-fed rats was markedly stimulated by adrenaline (glucose uptake was increased 3-fold and the production of CO2 and the glycerol moiety of acylglycerol was increased 4-fold). However, adipose tissue from fat-fed rats was resistant to the effect of insulin to produce a further increase in adrenaline-stimulated glucose uptake. The intracellular capacity of lipogenesis on the one hand, and the production of CO2 and the glycerol moiety of acylglycerol on the other, are of prime importance in the action of insulin and adrenaline on glucose utilization in this model."}
{"id": "PMID:486120", "title": "The subcellular fractionation of embryonic chick tendon and cartilage cells: a re-examination.", "content": "A re-examination of the subcellular fractions obtained from matrix-free chick tendon and cartilage cells has been made since the discovery that three out of four of the micrographs of chick tendon microsomal fractions published in an earlier paper from this laboratory were not authentic. The present studies demonstrate that by using the procedures previously reported it is possible to isolate microsomal and submicrosomal fractions from tendon and cartilage cells which exhibit typical morphology when examined by electron microscopy. These observations are consistent with our original biochemical characterization of subcellular fractions, which we know to be valid. Other publications from this laboratory in which these fractionation procedures have been applied to studies of collagen biosynthesis are in no way compromised, and indeed, most of our data have been confirmed by several other laboratories.", "contents": "The subcellular fractionation of embryonic chick tendon and cartilage cells: a re-examination. A re-examination of the subcellular fractions obtained from matrix-free chick tendon and cartilage cells has been made since the discovery that three out of four of the micrographs of chick tendon microsomal fractions published in an earlier paper from this laboratory were not authentic. The present studies demonstrate that by using the procedures previously reported it is possible to isolate microsomal and submicrosomal fractions from tendon and cartilage cells which exhibit typical morphology when examined by electron microscopy. These observations are consistent with our original biochemical characterization of subcellular fractions, which we know to be valid. Other publications from this laboratory in which these fractionation procedures have been applied to studies of collagen biosynthesis are in no way compromised, and indeed, most of our data have been confirmed by several other laboratories."}
{"id": "PMID:486121", "title": "The separation of microsomal membranes and free polyribosomes by gel filtration.", "content": "The integrity of polyribosome-membrane complexes in microsomal preparations is dependent on the centrifugal conditions used for their preparation. This paper describes techniques based on gel filtration that enable the rapid and gentle separation of microsomal membranes from both free polyribosomes and soluble protein.", "contents": "The separation of microsomal membranes and free polyribosomes by gel filtration. The integrity of polyribosome-membrane complexes in microsomal preparations is dependent on the centrifugal conditions used for their preparation. This paper describes techniques based on gel filtration that enable the rapid and gentle separation of microsomal membranes from both free polyribosomes and soluble protein."}
{"id": "PMID:486122", "title": "The rapid preparation of peroxisomes from rat liver by using a vertical rotor.", "content": "A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.", "contents": "The rapid preparation of peroxisomes from rat liver by using a vertical rotor. A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes."}
{"id": "PMID:486123", "title": "Subcellular localization of the enzyme that forms mannosyl retinyl phosphate from guanosine diphosphate [14C]mannose and retinyl phosphate.", "content": "The subcellular distribution of the enzyme catalysing the conversion of retinyl phosphate and GDP-[14C]mannose into [14C]mannosyl retinyl phosphate was determined by using subcellular fractions of rat liver. Purity of fractions, as determined by marker enzymes, was 80% or better. The amount of mannosyl retinyl phosphate formed (pmol/min per mg of protein) for each fraction was: rough endoplasmic reticulum 0.48 +/- 0.09 (mean +/- S.D.); smooth membranes (consisting of 60% smooth endoplasmic reticulum and 40% Golgi apparatus), 0.18 +/- 0.03; Golgi apparatus, 0.13 +/- 0.03; and plasma membrane 0.02.", "contents": "Subcellular localization of the enzyme that forms mannosyl retinyl phosphate from guanosine diphosphate [14C]mannose and retinyl phosphate. The subcellular distribution of the enzyme catalysing the conversion of retinyl phosphate and GDP-[14C]mannose into [14C]mannosyl retinyl phosphate was determined by using subcellular fractions of rat liver. Purity of fractions, as determined by marker enzymes, was 80% or better. The amount of mannosyl retinyl phosphate formed (pmol/min per mg of protein) for each fraction was: rough endoplasmic reticulum 0.48 +/- 0.09 (mean +/- S.D.); smooth membranes (consisting of 60% smooth endoplasmic reticulum and 40% Golgi apparatus), 0.18 +/- 0.03; Golgi apparatus, 0.13 +/- 0.03; and plasma membrane 0.02."}
{"id": "PMID:486124", "title": "Effect of dietary phosphate intake on phosphate transport by isolated rat renal brush-border vesicles.", "content": "Renal brush-border membrane vesicles isolated from rats kept for 6-8 weeks on a low-phosphate diet (0.15% of dry matter) showed a markedly faster Na(+)-dependent phosphate uptake than did membrane vesicles isolated from animals kept on a high-phosphate diet (2% of dry matter). Phosphate-uptake rate by brush-border membrane vesicles isolated from animals on a low-phosphate diet remained significantly increased after acute parathyroidectomy. Dietary adaptation was also observed in animals that had been parathyroidectomized before exposure to the different diets. In animals on the low-phosphate diet parathyrin administration inhibited phosphate uptake by brush-border vesicles only if the animals were repleted with P(i) (5ml of 20mm-NaH(2)PO(4)) 1h before being killed. After acute phosphate loading and parathyrin administration the difference in the transport rate between the two dietary groups remained statistically significant. The results suggest that the adaptation of proximal-tubule phosphate transport to dietary intake of phosphate is reflected in the Na(+)/phosphate co-transport system located in the luminal membrane of the proximal-tubule cell. Since the dietary effects on phosphate transport by brush-border membranes are only partially reversed by acute changes in parathyrin concentration and are also observed in chronically parathyroidectomized animals, the adaptation of the Na(+)/phosphate co-transport system to dietary phosphate intake seems to involve an additional mechanism independent of parathyrin.", "contents": "Effect of dietary phosphate intake on phosphate transport by isolated rat renal brush-border vesicles. Renal brush-border membrane vesicles isolated from rats kept for 6-8 weeks on a low-phosphate diet (0.15% of dry matter) showed a markedly faster Na(+)-dependent phosphate uptake than did membrane vesicles isolated from animals kept on a high-phosphate diet (2% of dry matter). Phosphate-uptake rate by brush-border membrane vesicles isolated from animals on a low-phosphate diet remained significantly increased after acute parathyroidectomy. Dietary adaptation was also observed in animals that had been parathyroidectomized before exposure to the different diets. In animals on the low-phosphate diet parathyrin administration inhibited phosphate uptake by brush-border vesicles only if the animals were repleted with P(i) (5ml of 20mm-NaH(2)PO(4)) 1h before being killed. After acute phosphate loading and parathyrin administration the difference in the transport rate between the two dietary groups remained statistically significant. The results suggest that the adaptation of proximal-tubule phosphate transport to dietary intake of phosphate is reflected in the Na(+)/phosphate co-transport system located in the luminal membrane of the proximal-tubule cell. Since the dietary effects on phosphate transport by brush-border membranes are only partially reversed by acute changes in parathyrin concentration and are also observed in chronically parathyroidectomized animals, the adaptation of the Na(+)/phosphate co-transport system to dietary phosphate intake seems to involve an additional mechanism independent of parathyrin."}
{"id": "PMID:486125", "title": "Ligation and synthesis of chromatin deoxyribonucleic acid in vitro in neuronal, glial and liver nuclei isolated from adult guinea pig.", "content": "Neuron-rich and glial nuclear preparations and liver nuclei were isolated from adult guinea pigs. These nuclei were incubated to carry out DNA-ligation and -synthesis reactions. Before and after incubation, the sizes of single-standed DNA and DNA-synthesis patterns in single strands were analysed by using alkaline sucrose-density-gradient centrifugation. Isolation of nuclei by cell-fractionation technique shortened chromatin DNA and decreased markedly the number-average molecular weight of DNA strands. Chromatin DNA in neuronal and glial nuclei was ligated at the nicks during incubation in a reaction mixture containing ATP, Mg(2+), dithiothreitol and four deoxyribonucleotides. The number-average molecular weights were estimated to increase 1.1-and 2.1-fold in neuronal and glial nuclei respectively. DNA strands in liver nuclei were shortened during incubation, but elongated under conditions that inhibit deoxyribonuclease. Since the endogenous deoxyribounuclease activity was conspicuously higher in liver nuclei than in neuronal and glial nuclei, the shortening and elongation were thought to depend on the balance between DNA ligase and deoxyribonuclease reactions. DNA synthesis occurred at the gaps in chromatin DNA and about 50% of the total synthesized DNA was found in the shorter strands having 6 to 297 bases in all species of nuclei. Based on these results, it was concluded that in nuclei isolated from non-dividing cells (neurons) and slowly dividing cells (glial and liver cells) DNA-ligation and -synthesis reactions proceeded in parallel at the breaks in single-stranded DNA, which was produced mainly by endogenous deoxyribonuclease during isolation and incubation processes.", "contents": "Ligation and synthesis of chromatin deoxyribonucleic acid in vitro in neuronal, glial and liver nuclei isolated from adult guinea pig. Neuron-rich and glial nuclear preparations and liver nuclei were isolated from adult guinea pigs. These nuclei were incubated to carry out DNA-ligation and -synthesis reactions. Before and after incubation, the sizes of single-standed DNA and DNA-synthesis patterns in single strands were analysed by using alkaline sucrose-density-gradient centrifugation. Isolation of nuclei by cell-fractionation technique shortened chromatin DNA and decreased markedly the number-average molecular weight of DNA strands. Chromatin DNA in neuronal and glial nuclei was ligated at the nicks during incubation in a reaction mixture containing ATP, Mg(2+), dithiothreitol and four deoxyribonucleotides. The number-average molecular weights were estimated to increase 1.1-and 2.1-fold in neuronal and glial nuclei respectively. DNA strands in liver nuclei were shortened during incubation, but elongated under conditions that inhibit deoxyribonuclease. Since the endogenous deoxyribounuclease activity was conspicuously higher in liver nuclei than in neuronal and glial nuclei, the shortening and elongation were thought to depend on the balance between DNA ligase and deoxyribonuclease reactions. DNA synthesis occurred at the gaps in chromatin DNA and about 50% of the total synthesized DNA was found in the shorter strands having 6 to 297 bases in all species of nuclei. Based on these results, it was concluded that in nuclei isolated from non-dividing cells (neurons) and slowly dividing cells (glial and liver cells) DNA-ligation and -synthesis reactions proceeded in parallel at the breaks in single-stranded DNA, which was produced mainly by endogenous deoxyribonuclease during isolation and incubation processes."}
{"id": "PMID:486126", "title": "The effect of tunicamycin on the glycosylation of lactating-rabbit mammary glycoproteins.", "content": "1. Tunicamycin inhibited the incorporation of d-[2-(3)H]mannose into dolichol-linked oligosaccharide and glycoprotein of lactating-rabbit mammary explants by approximately the same extent (approx. 30% of control value), suggesting that lipid-linked intermediates are involved in the mannosylation of mammary glycoproteins. 2. The incorporation of radioactivity from N-acetyl-d-[1-(14)C]glucosamine into dolichol-linked oligosaccharide was inhibited by tunicamycin to 32% of the control value, whereas the incorporation of the radiolabel into glycoprotein was only inhibited to 72% of the control value. 3. Considerable redistribution of label from N-acetylglucosamine to N-acetylgalactosamine was found to occur in the explants. In the presence of tunicamycin approx. 76% of the radioactivity incorporated into glycoprotein from N-acetyl-d-[1-(14)C]glucosamine was present as N-acetylgalactosamine, compared with approx. 61% in the absence of the inhibitor. Thus tunicamycin selectively inhibits the incorporation of N-acetylglucosamine into glycoprotein. 4. Radioactivity from N-acetyl-d-[1-(14)C]glucosamine was incorporated into a glycoprotein that was identified as casein by the use of a casein-specific antiserum, and also into a group of glycopolypeptides with apparent mol.wts. ranging between 40000 and 80000. N-Acetylgalactosamine was the only radioactive sugar released on strong-acid hydrolysis of the immunoprecipitated casein, whereas N-acetylglucosamine was the major radioactive residue present in the non-casein glycoproteins. Glucosamine and galactosamine were the only radiolabelled sugars detected by paper chromatography of the strong-acid hydrolysate of the protein fraction. 5. Tunicamycin inhibited the incorporation of radioactivity from N-acetyl-d-[1-(14)C]glucosamine into the glycopolypeptides with mol.wts. between 40000 and 80000 as described by polyacrylamide-gel electrophoresis, but did not affect the incorporation of label into casein. It appears that tunicamycin inhibits the incorporation of mannose and N-acetylglucosamine into a number of mammary glycoproteins by inhibiting the formation of lipid-linked intermediates, but does not inhibit the incorporation of N-acetylgalactosamine into casein.", "contents": "The effect of tunicamycin on the glycosylation of lactating-rabbit mammary glycoproteins. 1. Tunicamycin inhibited the incorporation of d-[2-(3)H]mannose into dolichol-linked oligosaccharide and glycoprotein of lactating-rabbit mammary explants by approximately the same extent (approx. 30% of control value), suggesting that lipid-linked intermediates are involved in the mannosylation of mammary glycoproteins. 2. The incorporation of radioactivity from N-acetyl-d-[1-(14)C]glucosamine into dolichol-linked oligosaccharide was inhibited by tunicamycin to 32% of the control value, whereas the incorporation of the radiolabel into glycoprotein was only inhibited to 72% of the control value. 3. Considerable redistribution of label from N-acetylglucosamine to N-acetylgalactosamine was found to occur in the explants. In the presence of tunicamycin approx. 76% of the radioactivity incorporated into glycoprotein from N-acetyl-d-[1-(14)C]glucosamine was present as N-acetylgalactosamine, compared with approx. 61% in the absence of the inhibitor. Thus tunicamycin selectively inhibits the incorporation of N-acetylglucosamine into glycoprotein. 4. Radioactivity from N-acetyl-d-[1-(14)C]glucosamine was incorporated into a glycoprotein that was identified as casein by the use of a casein-specific antiserum, and also into a group of glycopolypeptides with apparent mol.wts. ranging between 40000 and 80000. N-Acetylgalactosamine was the only radioactive sugar released on strong-acid hydrolysis of the immunoprecipitated casein, whereas N-acetylglucosamine was the major radioactive residue present in the non-casein glycoproteins. Glucosamine and galactosamine were the only radiolabelled sugars detected by paper chromatography of the strong-acid hydrolysate of the protein fraction. 5. Tunicamycin inhibited the incorporation of radioactivity from N-acetyl-d-[1-(14)C]glucosamine into the glycopolypeptides with mol.wts. between 40000 and 80000 as described by polyacrylamide-gel electrophoresis, but did not affect the incorporation of label into casein. It appears that tunicamycin inhibits the incorporation of mannose and N-acetylglucosamine into a number of mammary glycoproteins by inhibiting the formation of lipid-linked intermediates, but does not inhibit the incorporation of N-acetylgalactosamine into casein."}
{"id": "PMID:486127", "title": "Hormonal control of uteroglobin secretion in rabbit uterus: inhibition of uteroglobin synthesis and messenger ribonucleic acid accumulation by oestrogen and anti-oestrogen administration.", "content": "Investigations were conducted to quantify activity of uteroglobin mRNA and secretion of uteroglobin in rabbit uterus after administration of progesterone and 5alpha-dihydrotestosterone, either alone or concomitantly with oestradiol-17beta and tamoxifen, a non-steroidal anti-oestrogen. Poly(A)-containing mRNA was isolated from the uterine tissue by extraction with phenol/chloroform, precipitation with ethanol and chromatography on oligo(dT)-cellulose. Cell-free translation in vitro of the poly(A)-containing mRNA was carried out in a wheat-germ lysate, and the product isolated by specific immuno-precipitation with anti-uteroglobin antiserum purified by affinity chromatography. Radioimmunoassay was utilized to determine uteroglobin content in the uterine flushings and tissue preparations. When given for 5 days, both progesterone (1mg/kg per day) and 5alpha-dihydrotestosterone (25mg/kg per day) elicited a marked induction of uteroglobin secretion, which was accompanied with accumulation of uteroglobin mRNA in the tissue. Concomitant administration of oestradiol-17beta (50mug/kg per day) or tamoxifen (12.5mg/kg per day) significantly decreased both progesterone- and 5alpha-dihydrotestosterone-induced uteroglobin secretion, with a parallel decrease in the uteroglobin-mRNA activity. The decline in the uteroglobin content of the uterine flushes brought about by oestradiol-17beta or tamoxifen administration was not due to inhibition of secretion of this protein by the endometrial cells, since a simultaneous decrease occurred in the tissue uteroglobin content. After a 5-day pretreatment with progesterone (1mg/kg per day), administration of oestradiol-17beta (50mug/kg per day) during the ensuing 4 days greatly accelerated the decay of the uteroglobin content in the uterine fluid.", "contents": "Hormonal control of uteroglobin secretion in rabbit uterus: inhibition of uteroglobin synthesis and messenger ribonucleic acid accumulation by oestrogen and anti-oestrogen administration. Investigations were conducted to quantify activity of uteroglobin mRNA and secretion of uteroglobin in rabbit uterus after administration of progesterone and 5alpha-dihydrotestosterone, either alone or concomitantly with oestradiol-17beta and tamoxifen, a non-steroidal anti-oestrogen. Poly(A)-containing mRNA was isolated from the uterine tissue by extraction with phenol/chloroform, precipitation with ethanol and chromatography on oligo(dT)-cellulose. Cell-free translation in vitro of the poly(A)-containing mRNA was carried out in a wheat-germ lysate, and the product isolated by specific immuno-precipitation with anti-uteroglobin antiserum purified by affinity chromatography. Radioimmunoassay was utilized to determine uteroglobin content in the uterine flushings and tissue preparations. When given for 5 days, both progesterone (1mg/kg per day) and 5alpha-dihydrotestosterone (25mg/kg per day) elicited a marked induction of uteroglobin secretion, which was accompanied with accumulation of uteroglobin mRNA in the tissue. Concomitant administration of oestradiol-17beta (50mug/kg per day) or tamoxifen (12.5mg/kg per day) significantly decreased both progesterone- and 5alpha-dihydrotestosterone-induced uteroglobin secretion, with a parallel decrease in the uteroglobin-mRNA activity. The decline in the uteroglobin content of the uterine flushes brought about by oestradiol-17beta or tamoxifen administration was not due to inhibition of secretion of this protein by the endometrial cells, since a simultaneous decrease occurred in the tissue uteroglobin content. After a 5-day pretreatment with progesterone (1mg/kg per day), administration of oestradiol-17beta (50mug/kg per day) during the ensuing 4 days greatly accelerated the decay of the uteroglobin content in the uterine fluid."}
{"id": "PMID:486128", "title": "Demonstration of altered acidic hydrolases in fibroblasts from patients with mucolipidosis II by lectin titration.", "content": "Decreased binding by the lectins concanavalin A and wheat-germ agglutinin was found for a number of acidic hydrolases from skin fibroblasts of three unrelated patients with mucolipidosis II. This decreased binding as compared with normal controls was demonstrated by titration of hydrolase activities with increasing amounts of immobilized lectins. Neuraminidase treatment slightly improved the binding of enzymes from mucolipidosis-II patients, in contrast with the diminished binding found or hydrolases from control cell lines. The abnormality in binding by lectins of hydrolases of mucolipidosis-II patients was observed for enzymes with various degrees of intracellular deficiency as well as for enzymes with normal intracellular activities. These findings suggest a generalized alteration of fibroblast acidic hydrolase molecules in mucolipidosis II.", "contents": "Demonstration of altered acidic hydrolases in fibroblasts from patients with mucolipidosis II by lectin titration. Decreased binding by the lectins concanavalin A and wheat-germ agglutinin was found for a number of acidic hydrolases from skin fibroblasts of three unrelated patients with mucolipidosis II. This decreased binding as compared with normal controls was demonstrated by titration of hydrolase activities with increasing amounts of immobilized lectins. Neuraminidase treatment slightly improved the binding of enzymes from mucolipidosis-II patients, in contrast with the diminished binding found or hydrolases from control cell lines. The abnormality in binding by lectins of hydrolases of mucolipidosis-II patients was observed for enzymes with various degrees of intracellular deficiency as well as for enzymes with normal intracellular activities. These findings suggest a generalized alteration of fibroblast acidic hydrolase molecules in mucolipidosis II."}
{"id": "PMID:486129", "title": "Effects of alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor or ornithine decarboxylase, on testosterone-induced regeneration of prostate and seminal vesicle in castrated rats.", "content": "1. Castration of adult rats markedly decreases the amounts of polyamines (putrescine, spermidine and spermine) and of RNA and DNA in the ventral prostate and the seminal vesicle. 2. Daily injections of testosterone propionate to rats castrated 7 days previously increase polyamine and nucleic acid contents more rapidly in the seminal vesicle than in the ventral prostate. 3. After 7 days of androgen treatment, polyamine and nucleic acid contents of the seminal vesicle are significantly higher than those of intact animals. Nucleic acid, but not polyamine, contents return to normal values during the next 4 days of continued treatment. In the prostate, androgen treatment increases polyamine and nucleic acid contents to, but not above, normal values. 4. Repeated doses of alpha-difluoromethylornithine, a potent enzyme-activated irreversible inhibitor of ornithine decarboxylase, totally blocked the testosterone-induced increase of putrescine and spermidine in the ventral prostate and of putrescine in the seminal vesicle. They slowed significantly the accumulation of spermine in the ventral prostate and of spermidine in the seminal vesicle. alpha-Difluoromethylornithine also retarded the testosterone-induced accumulation of RNA in the ventral prostate. However, no clear correlation was apparent between accumulation of polyamines and of nucleic acids in the two organs. 5. alpha-Difluoromethylornithine markedly slows the testosterone-induced weight gain of the prostate, but not of the seminal vesicle. Cytological studies suggest that this effect on the prostate is due to inhibition of the androgen-induced restoration of the secretion content of prostatic acini.", "contents": "Effects of alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor or ornithine decarboxylase, on testosterone-induced regeneration of prostate and seminal vesicle in castrated rats. 1. Castration of adult rats markedly decreases the amounts of polyamines (putrescine, spermidine and spermine) and of RNA and DNA in the ventral prostate and the seminal vesicle. 2. Daily injections of testosterone propionate to rats castrated 7 days previously increase polyamine and nucleic acid contents more rapidly in the seminal vesicle than in the ventral prostate. 3. After 7 days of androgen treatment, polyamine and nucleic acid contents of the seminal vesicle are significantly higher than those of intact animals. Nucleic acid, but not polyamine, contents return to normal values during the next 4 days of continued treatment. In the prostate, androgen treatment increases polyamine and nucleic acid contents to, but not above, normal values. 4. Repeated doses of alpha-difluoromethylornithine, a potent enzyme-activated irreversible inhibitor of ornithine decarboxylase, totally blocked the testosterone-induced increase of putrescine and spermidine in the ventral prostate and of putrescine in the seminal vesicle. They slowed significantly the accumulation of spermine in the ventral prostate and of spermidine in the seminal vesicle. alpha-Difluoromethylornithine also retarded the testosterone-induced accumulation of RNA in the ventral prostate. However, no clear correlation was apparent between accumulation of polyamines and of nucleic acids in the two organs. 5. alpha-Difluoromethylornithine markedly slows the testosterone-induced weight gain of the prostate, but not of the seminal vesicle. Cytological studies suggest that this effect on the prostate is due to inhibition of the androgen-induced restoration of the secretion content of prostatic acini."}
{"id": "PMID:486130", "title": "The decarboxylation of S-adenosylmethionine by detergent-treated extracts of rat liver.", "content": "1. The production of (14)CO(2) from S-adenosyl[carboxyl-(14)C]methionine by rat liver extracts was investigated. It was found that, in addition to the well-known cytosolic putrescine-activated S-adenosylmethionine decarboxylase, an activity carrying out the production of (14)CO(2) could be extracted from a latent, particulate or membrane-bound form by treatment with buffer containing 1% (v/v) Triton X-100 [confirming the report of Sturman (1976) Biochim. Biophys. Acta428, 56-69]. 2. The formation of (14)CO(2) by such detergent-solubilized extracts differed from that by cytosolic S-adenosylmethionine decarboxylase in a number of ways. The reaction by the solubilized extracts did not require putrescine and was not directly proportional to time of incubation or the amount of protein added. Instead, activity a showed a distinct lag period and was much greater when high concentrations of the extracts were used. The cytosolic S-adenosylmethionine decarboxylase was activated by putrescine, showed strict proportionality to protein added and the reaction proceeded at a constant rate. Cytosolic activity was not inhibited by homoserine or by S-adenosylhomocysteine, whereas the Triton-solubilized activity was strongly inhibited. 3. By using an acetone precipitate of Triton-treated homogenates as a source of the activity, it was found that decarboxylated S-adenosylmethionine was not present among the products of the reaction, although 5'-methylthioadenosine and 5-methylthioribose were found. Such extracts were able to produce (14)CO(2) when incubated with [U-(14)C]-homoserine, and (14)CO(2) production was greater when S-adenosyl[carboxyl-(14)C]methionine that had been degraded by heating at pH6 at 100 degrees C for 30min (a procedure known to produce mainly 5'-methylthioadenosine and homoserine lactone) was used as a substrate than when S-adenosyl[carboxyl-(14)C]methionine was used. 4. These results indicate that the Triton-solubilized activity is not a real S-adenosylmethionine decarboxylase, but that (14)CO(2) is produced via a series of reactions involving degradation of the S-adenosyl-[carboxyl-(14)C]methionine. It is probable that this degradation can occur via several pathways. Our results would suggest that part of the reaction occurs via the production of S-adenosylhomocysteine, which can then be converted into 2-oxobutyrate via the transsulphuration pathway, and that part occurs via the production of homoserine by an enzyme converting S-adenosylmethionine into 5'-methylthioadenosine and homoserine lactone.", "contents": "The decarboxylation of S-adenosylmethionine by detergent-treated extracts of rat liver. 1. The production of (14)CO(2) from S-adenosyl[carboxyl-(14)C]methionine by rat liver extracts was investigated. It was found that, in addition to the well-known cytosolic putrescine-activated S-adenosylmethionine decarboxylase, an activity carrying out the production of (14)CO(2) could be extracted from a latent, particulate or membrane-bound form by treatment with buffer containing 1% (v/v) Triton X-100 [confirming the report of Sturman (1976) Biochim. Biophys. Acta428, 56-69]. 2. The formation of (14)CO(2) by such detergent-solubilized extracts differed from that by cytosolic S-adenosylmethionine decarboxylase in a number of ways. The reaction by the solubilized extracts did not require putrescine and was not directly proportional to time of incubation or the amount of protein added. Instead, activity a showed a distinct lag period and was much greater when high concentrations of the extracts were used. The cytosolic S-adenosylmethionine decarboxylase was activated by putrescine, showed strict proportionality to protein added and the reaction proceeded at a constant rate. Cytosolic activity was not inhibited by homoserine or by S-adenosylhomocysteine, whereas the Triton-solubilized activity was strongly inhibited. 3. By using an acetone precipitate of Triton-treated homogenates as a source of the activity, it was found that decarboxylated S-adenosylmethionine was not present among the products of the reaction, although 5'-methylthioadenosine and 5-methylthioribose were found. Such extracts were able to produce (14)CO(2) when incubated with [U-(14)C]-homoserine, and (14)CO(2) production was greater when S-adenosyl[carboxyl-(14)C]methionine that had been degraded by heating at pH6 at 100 degrees C for 30min (a procedure known to produce mainly 5'-methylthioadenosine and homoserine lactone) was used as a substrate than when S-adenosyl[carboxyl-(14)C]methionine was used. 4. These results indicate that the Triton-solubilized activity is not a real S-adenosylmethionine decarboxylase, but that (14)CO(2) is produced via a series of reactions involving degradation of the S-adenosyl-[carboxyl-(14)C]methionine. It is probable that this degradation can occur via several pathways. Our results would suggest that part of the reaction occurs via the production of S-adenosylhomocysteine, which can then be converted into 2-oxobutyrate via the transsulphuration pathway, and that part occurs via the production of homoserine by an enzyme converting S-adenosylmethionine into 5'-methylthioadenosine and homoserine lactone."}
{"id": "PMID:486131", "title": "Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin.", "content": "1. Evidence is presented that exposure of epididymal fat-pads from fed rats to insulin leads to a marked diminution in the Km for phosphoenolpyruvate of pyruvate kinase. Effects of insulin may be readily demonstrated in experiments both in vivo and in vitro and are not secondary to the activation by the hormone of glucose transport. No effect of insulin is apparent in tissues from 48 h-starved animals. 2. The mechanism of the effect of insulin on pyruvate kinase was not established. The observed changes in Km do not appear to be the result of alterations in the amounts of bound effectors such as fructose 1,6-bisphosphate and alanine. Rather, as the effect persists in incubated extracts, it appears that a change in the degree of phosphorylation or some other covalent modification of the enzyme may be involved.", "contents": "Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin. 1. Evidence is presented that exposure of epididymal fat-pads from fed rats to insulin leads to a marked diminution in the Km for phosphoenolpyruvate of pyruvate kinase. Effects of insulin may be readily demonstrated in experiments both in vivo and in vitro and are not secondary to the activation by the hormone of glucose transport. No effect of insulin is apparent in tissues from 48 h-starved animals. 2. The mechanism of the effect of insulin on pyruvate kinase was not established. The observed changes in Km do not appear to be the result of alterations in the amounts of bound effectors such as fructose 1,6-bisphosphate and alanine. Rather, as the effect persists in incubated extracts, it appears that a change in the degree of phosphorylation or some other covalent modification of the enzyme may be involved."}
{"id": "PMID:486132", "title": "The low availability of dietary choline for the nutrition of the sheep.", "content": "1. Choline, which is present in the diet of the sheep either in the non-esterified form or combined in phospholipids, is rapidly degraded in the rumen. The ultimate product formed from the N-methyl groups is methane. 2. Analysis of the non-esterified choline and the phosphatidylcholine in ruminal and abomasal digesta indicate that the phospholipid is the main vehicle for the passage of choline to the lower digestive tract. 3. The concentration of phosphatidylcholine in abomasal digesta is lower than that of ruminal digesta, which is in line with a selective retention of protozoa in the rumen as observed by others. 4. On defaunation of the rumen to remove ciliated protozoa the concentration of phosphatidylcholine in ruminal digesta falls markedly and becomes lower than that in abomasal digesta. 5. Calculation shows that the adult sheep obtains at most only about 20--25 mg of effective choline per day from its diet (0.002--0.0025% of dietary total dry-weight intake). This is some fifty times less than the minimum required to avoid pathological lesions and death in other species investigated (0.1%+ of dietary dry-weight intake). 6. Sheep liver can synthesize choline from [14C]ethanolamine both in vitro and in vivo, but the synthesis of choline per kg body weight is many times less than it is in the rat. 7. The intact sheep oxidizes an injected dose of [1,2-14C]choline to CO2 at a rate that is several times less than that observed for the rat. This could help to explain the apparent minimal requirement of sheep for dietary choline.", "contents": "The low availability of dietary choline for the nutrition of the sheep. 1. Choline, which is present in the diet of the sheep either in the non-esterified form or combined in phospholipids, is rapidly degraded in the rumen. The ultimate product formed from the N-methyl groups is methane. 2. Analysis of the non-esterified choline and the phosphatidylcholine in ruminal and abomasal digesta indicate that the phospholipid is the main vehicle for the passage of choline to the lower digestive tract. 3. The concentration of phosphatidylcholine in abomasal digesta is lower than that of ruminal digesta, which is in line with a selective retention of protozoa in the rumen as observed by others. 4. On defaunation of the rumen to remove ciliated protozoa the concentration of phosphatidylcholine in ruminal digesta falls markedly and becomes lower than that in abomasal digesta. 5. Calculation shows that the adult sheep obtains at most only about 20--25 mg of effective choline per day from its diet (0.002--0.0025% of dietary total dry-weight intake). This is some fifty times less than the minimum required to avoid pathological lesions and death in other species investigated (0.1%+ of dietary dry-weight intake). 6. Sheep liver can synthesize choline from [14C]ethanolamine both in vitro and in vivo, but the synthesis of choline per kg body weight is many times less than it is in the rat. 7. The intact sheep oxidizes an injected dose of [1,2-14C]choline to CO2 at a rate that is several times less than that observed for the rat. This could help to explain the apparent minimal requirement of sheep for dietary choline."}
{"id": "PMID:486133", "title": "Effects of temperature, metabolic inhibitors and some other factors on fluid-phase and adsorptive pinocytosis by rat peritoneal macrophages.", "content": "Low temperature, NaF and 2,4-dinitrophenol could each abolish the pinocytic uptake of 125I-labelled poly(vinylpyrrolidone) or colloidal [198Au]gold by rat peritoneal macrophages cultured in vitro. Cytochalasin B caused only partial inhibition, even at 10 microgram/ml, and colchicine (10 or 25 microgram/ml) inhibited uptake of colloidal [198Au]gold much more than that of 125I-labelled poly(vinylpyrrolidone). Dibutyryl cyclic AMP and ouabain were without effect on uptake of 125I-labelled poly(vinylpyrrolidone), and slight stimulation was seen with ATP and theophylline. Uptake of 125I-labelled poly(vinylpyrrolidone) was abolished by EGTA (5mM), but restored by adding CaCl2 (5mM). The results appear not to support the conventional criteria for the division of pinocytic phenomena into macropinocytosis, requiring a metabolic energy supply and cytoskeletal components, and micropinocytosis, requiring neither.", "contents": "Effects of temperature, metabolic inhibitors and some other factors on fluid-phase and adsorptive pinocytosis by rat peritoneal macrophages. Low temperature, NaF and 2,4-dinitrophenol could each abolish the pinocytic uptake of 125I-labelled poly(vinylpyrrolidone) or colloidal [198Au]gold by rat peritoneal macrophages cultured in vitro. Cytochalasin B caused only partial inhibition, even at 10 microgram/ml, and colchicine (10 or 25 microgram/ml) inhibited uptake of colloidal [198Au]gold much more than that of 125I-labelled poly(vinylpyrrolidone). Dibutyryl cyclic AMP and ouabain were without effect on uptake of 125I-labelled poly(vinylpyrrolidone), and slight stimulation was seen with ATP and theophylline. Uptake of 125I-labelled poly(vinylpyrrolidone) was abolished by EGTA (5mM), but restored by adding CaCl2 (5mM). The results appear not to support the conventional criteria for the division of pinocytic phenomena into macropinocytosis, requiring a metabolic energy supply and cytoskeletal components, and micropinocytosis, requiring neither."}
{"id": "PMID:486134", "title": "Ability of human chorionic gonadotropin beta-subunit to inhibit the steroidogenic response to lutropin.", "content": "Ability of the beta-subunit of human chorionic gonadotropin to inhibit the response to lutropin (luteinizing hormone, LH) was tested in the immature rat ovarian system and pregnant-mare-serum-gonadotropin-primed rat ovarian system with progesterone production being used as the response. Human chorionic gonadotropin beta-subunit was found to inhibit human and ovine lutropin-stimulated progesterone production. At a constant dose of lutropin, inhibition was dependent on the concentration of beta-subunit. When concentration of the beta-subunit was kept constant at 5.0 microgram/ml and the concentration of lutropin was varied, the inhibition was maximum at the saturating concentration of the native hormone. The alpha-subunit of the human chorionic gonadotropin did not inhibit the response to lutropin. The lutropin/beta-subunit ratio required to produce an inhibition of response was much lower than that required to bring about an observable inhibition of binding.", "contents": "Ability of human chorionic gonadotropin beta-subunit to inhibit the steroidogenic response to lutropin. Ability of the beta-subunit of human chorionic gonadotropin to inhibit the response to lutropin (luteinizing hormone, LH) was tested in the immature rat ovarian system and pregnant-mare-serum-gonadotropin-primed rat ovarian system with progesterone production being used as the response. Human chorionic gonadotropin beta-subunit was found to inhibit human and ovine lutropin-stimulated progesterone production. At a constant dose of lutropin, inhibition was dependent on the concentration of beta-subunit. When concentration of the beta-subunit was kept constant at 5.0 microgram/ml and the concentration of lutropin was varied, the inhibition was maximum at the saturating concentration of the native hormone. The alpha-subunit of the human chorionic gonadotropin did not inhibit the response to lutropin. The lutropin/beta-subunit ratio required to produce an inhibition of response was much lower than that required to bring about an observable inhibition of binding."}
{"id": "PMID:486135", "title": "A potassium ion diffusion potential causes adrenaline uptake in chromaffin-granule 'ghosts'.", "content": "Membrane vesicles ('ghosts') formed from bovine chromaffin granules accumulate adrenaline in response to a diffusion potential produced by adding K+ in the presence of valinomycin. This uptake occurs as a short (2--5 min) burst because of the transient nature of the diffusion potential. The potential-driven uptake is optimal at pH approximately 7.2, is inhibited by reserpine, and has an initial rate comparable with that of ATP-driven uptake. These results show that ATP-dependent adrenaline uptake may occur at least partly in response to the membrane potential generated by an electrogenic proton-translocating adenosine triphosphatase found in chromaffin-granule membranes.", "contents": "A potassium ion diffusion potential causes adrenaline uptake in chromaffin-granule 'ghosts'. Membrane vesicles ('ghosts') formed from bovine chromaffin granules accumulate adrenaline in response to a diffusion potential produced by adding K+ in the presence of valinomycin. This uptake occurs as a short (2--5 min) burst because of the transient nature of the diffusion potential. The potential-driven uptake is optimal at pH approximately 7.2, is inhibited by reserpine, and has an initial rate comparable with that of ATP-driven uptake. These results show that ATP-dependent adrenaline uptake may occur at least partly in response to the membrane potential generated by an electrogenic proton-translocating adenosine triphosphatase found in chromaffin-granule membranes."}
{"id": "PMID:486136", "title": "Inhibition by cycloheximide of degradation of cytochrome P-450 in primary cultures of adult rat liver parenchymal cells and in vivo.", "content": "Degradation of cytochrome P-450 was studied in adult rat liver parenchymal cells in primary monolayer culture. In cells incubated in standard culture medium, the amount of cytochrome P-450 decreased at an accelerated rate relative to either the rate of degradation of total protein in the cells or the turnover of cytochrome P-450 in vivo. This change was succeeded by a spontaneous increase in the activity of haem oxygenase, an enzyme system that converts haem into bilirubin in vitro, measured in extracts from the cultured cells. This finding suggests that the rate of cytochrome P-450 breakdown may be controlled by factor(s) other than the activity of haem oxygenase. The decline in cytochrome P-450 and the subsequent increase in haem oxygenase activity was prevented by incubation of hepatocytes in medium containing an inhibitor of protein synthesis such as cycloheximide, puromycin, actinomycin D, or azaserine. The effect of cycloheximide appeared to be due to decreased breakdown of microsomal (14)C-labelled haem. By contrast, cycloheximide was without effect on the degradation of total protein, measured either in homogenates or in microsomal fractions prepared from the cultured cells. These results suggest that the conditions of cell culture stimulate selective degradation of cytochrome P-450 by a process that is inhibited by cycloheximide and hence may require protein synthesis. The findings in culture were verified in parallel studies of cytochrome P-450 degradation in vivo. After administration of bromobenzene, the degradation of the haem moiety of cytochrome P-450 was accelerated in vivo in a manner resembling that observed in cultured hepatocytes. Administration of cycloheximide to either bromobenzene-treated rats or to untreated rats decreased the degradation of the haem moiety of cytochrome P-450. However, the drug failed to affect degradation of haem not associated with cytochrome P-450, suggesting that cycloheximide is not a general inhibitor of haem oxidation in the liver. These findings confirm that the catabolism of hepatic cytochrome P-450 haem is controlled by similar cycloheximide-sensitive processes in the basal steady state in vivo, as stimulated by bromobenzene in vivo, or in hepatocytes under the conditions of cell culture. We conclude that the rate-limiting step in this process appears to require protein synthesis and precedes cleavage of the haem ring.", "contents": "Inhibition by cycloheximide of degradation of cytochrome P-450 in primary cultures of adult rat liver parenchymal cells and in vivo. Degradation of cytochrome P-450 was studied in adult rat liver parenchymal cells in primary monolayer culture. In cells incubated in standard culture medium, the amount of cytochrome P-450 decreased at an accelerated rate relative to either the rate of degradation of total protein in the cells or the turnover of cytochrome P-450 in vivo. This change was succeeded by a spontaneous increase in the activity of haem oxygenase, an enzyme system that converts haem into bilirubin in vitro, measured in extracts from the cultured cells. This finding suggests that the rate of cytochrome P-450 breakdown may be controlled by factor(s) other than the activity of haem oxygenase. The decline in cytochrome P-450 and the subsequent increase in haem oxygenase activity was prevented by incubation of hepatocytes in medium containing an inhibitor of protein synthesis such as cycloheximide, puromycin, actinomycin D, or azaserine. The effect of cycloheximide appeared to be due to decreased breakdown of microsomal (14)C-labelled haem. By contrast, cycloheximide was without effect on the degradation of total protein, measured either in homogenates or in microsomal fractions prepared from the cultured cells. These results suggest that the conditions of cell culture stimulate selective degradation of cytochrome P-450 by a process that is inhibited by cycloheximide and hence may require protein synthesis. The findings in culture were verified in parallel studies of cytochrome P-450 degradation in vivo. After administration of bromobenzene, the degradation of the haem moiety of cytochrome P-450 was accelerated in vivo in a manner resembling that observed in cultured hepatocytes. Administration of cycloheximide to either bromobenzene-treated rats or to untreated rats decreased the degradation of the haem moiety of cytochrome P-450. However, the drug failed to affect degradation of haem not associated with cytochrome P-450, suggesting that cycloheximide is not a general inhibitor of haem oxidation in the liver. These findings confirm that the catabolism of hepatic cytochrome P-450 haem is controlled by similar cycloheximide-sensitive processes in the basal steady state in vivo, as stimulated by bromobenzene in vivo, or in hepatocytes under the conditions of cell culture. We conclude that the rate-limiting step in this process appears to require protein synthesis and precedes cleavage of the haem ring."}
{"id": "PMID:486137", "title": "The application of microcalorimetry to the assessment of metabolic efficiency in isolated rat hepatocytes.", "content": "1. Heat output by suspensions of isolated rat hepatocytes was determined by using a modified batch-type microcalorimeter. 2. The ratio of O(2) uptake (determined polarographically) to heat output was used to assess the metabolic efficiency of isolated hepatocytes. 3. Cells from starved or fed rats incubated in either bicarbonate-buffered physiological saline containing gelatin, or bicarbonate-buffered physiological saline containing amino acids, serum albumin and glucose showed no significant difference with respect to the ratio of O(2) uptake to heat output. 4. For liver cells from 24h-starved rats, the addition of 10mm-dihydroxyacetone and 2.5mm-fructose significantly decreased the ratio of O(2) uptake to heat output from 1.94+/-0.05 in the controls to 1.52+/-0.04 and 1.54+/-0.01mumol/J respectively. 5. Glucagon (1mum), which slightly increased both O(2) uptake and heat output, did not significantly alter the ratio. 6. The addition of extracellular 10mm-NH(4)Cl and urease to provide an energetically wasteful cycle by ensuring hydrolysis of newly synthesized urea, lowered the ratio of O(2) uptake to heat output from 1.81+/-0.08 to 1.47+/-0.06mumol/J, indicating a reduced metabolic efficiency. 7. Metabolic efficiency in rats of different dietary regimen, age and genetically based obesity was also assessed. No differences in the ratio of O(2) uptake to heat output were found between liver cell suspensions prepared from rats maintained on colony diet and high-fat diet or sucrose-rich diet nor between animals ranging from 38 to 179 days of age. Comparison of the ratio of liver cell O(2) uptake to heat output between homozygote Zucker fa/fa obese rats and their lean littermates showed no significant difference. 8. It is concluded that the ratio of O(2) uptake to heat output for isolated hepatocytes is relatively constant unless perturbed by conditions that markedly enhance substrate cycling.", "contents": "The application of microcalorimetry to the assessment of metabolic efficiency in isolated rat hepatocytes. 1. Heat output by suspensions of isolated rat hepatocytes was determined by using a modified batch-type microcalorimeter. 2. The ratio of O(2) uptake (determined polarographically) to heat output was used to assess the metabolic efficiency of isolated hepatocytes. 3. Cells from starved or fed rats incubated in either bicarbonate-buffered physiological saline containing gelatin, or bicarbonate-buffered physiological saline containing amino acids, serum albumin and glucose showed no significant difference with respect to the ratio of O(2) uptake to heat output. 4. For liver cells from 24h-starved rats, the addition of 10mm-dihydroxyacetone and 2.5mm-fructose significantly decreased the ratio of O(2) uptake to heat output from 1.94+/-0.05 in the controls to 1.52+/-0.04 and 1.54+/-0.01mumol/J respectively. 5. Glucagon (1mum), which slightly increased both O(2) uptake and heat output, did not significantly alter the ratio. 6. The addition of extracellular 10mm-NH(4)Cl and urease to provide an energetically wasteful cycle by ensuring hydrolysis of newly synthesized urea, lowered the ratio of O(2) uptake to heat output from 1.81+/-0.08 to 1.47+/-0.06mumol/J, indicating a reduced metabolic efficiency. 7. Metabolic efficiency in rats of different dietary regimen, age and genetically based obesity was also assessed. No differences in the ratio of O(2) uptake to heat output were found between liver cell suspensions prepared from rats maintained on colony diet and high-fat diet or sucrose-rich diet nor between animals ranging from 38 to 179 days of age. Comparison of the ratio of liver cell O(2) uptake to heat output between homozygote Zucker fa/fa obese rats and their lean littermates showed no significant difference. 8. It is concluded that the ratio of O(2) uptake to heat output for isolated hepatocytes is relatively constant unless perturbed by conditions that markedly enhance substrate cycling."}
{"id": "PMID:486138", "title": "Metabolism of dibenzo[1,4]dioxan by a Pseudomonas species.", "content": "Pseudomonas sp. N.C.I.B. 9816 strain 11, when grown on salicylate in the presence of dibenzo[1,4]dioxan, accumulated cis-1,2-dihydroxy-1,2-dihydrodibenzo[1,4]dioxan and 2-hydroxydibenzo[1,4]dioxan in the culture medium. Each metabolite was isolated in crystalline form and identified by a variety of conventional chemical techniques. Crude cell extracts prepared from the parental strain grown with naphthalene oxidized cis-1,2-dihydroxy-1,2-dihydrodibenzo[1,4]dioxan under both aerobic and anaerobic conditions to 1,2-dihydroxydibenzo[1,4]dioxan. Further degradation of this metabolite was not detected.", "contents": "Metabolism of dibenzo[1,4]dioxan by a Pseudomonas species. Pseudomonas sp. N.C.I.B. 9816 strain 11, when grown on salicylate in the presence of dibenzo[1,4]dioxan, accumulated cis-1,2-dihydroxy-1,2-dihydrodibenzo[1,4]dioxan and 2-hydroxydibenzo[1,4]dioxan in the culture medium. Each metabolite was isolated in crystalline form and identified by a variety of conventional chemical techniques. Crude cell extracts prepared from the parental strain grown with naphthalene oxidized cis-1,2-dihydroxy-1,2-dihydrodibenzo[1,4]dioxan under both aerobic and anaerobic conditions to 1,2-dihydroxydibenzo[1,4]dioxan. Further degradation of this metabolite was not detected."}
{"id": "PMID:486139", "title": "Relationship between phosphatidylinositol synthesis and recovery of 5-hydroxytryptamine-responsive Ca2+ flux in blowfly salivary glands.", "content": "Each salivary gland contains about 135 pmol of phosphatidylinositol. In glands prelabelled by incubation for 1 h with [32P]Pi or [3H]inositol there was a subsequent breakdown of 80% of the labelled phosphatidylinositol over a 2 h incubation period with 10 micrometer-5-hydroxytryptamine. However, there was no detectable decrease either in total phosphatidylinositol based on phosphorus analysis by chemical estimation or in the radioactivity of [32P]phosphatidylinositol in salivary glands of flies raised from the larval stage on diets containing[32P]Pi and whose phospholipids were uniformly labelled. These results suggest that the pool of phosphatidylinositol involved with Ca2+ gating is a small fraction of the total phosphatidylinositol content. Furthermore it is this small compartment that is preferentially radioactively labelled during short-term incubations with radioactively labelled precursors. In salivary glands incubated for 2 h with 10 micrometer-5-hydroxytryptamine there was a marked decrease in the flux of 45Ca2+ across the gland. After removal of the hormone, incubation of salivary glands for 1 h in the presence of 2mM-inositol, but not choline or ethanolamine, resulted in a recovery of hormone-responsive 45Ca2+ flux. Quantitative studies revealed that less than 9 pmol of phosphatidylinositol must be formed to fully restoret he 5-hydroxytryptamine-responsive 45Ca2+ flux.", "contents": "Relationship between phosphatidylinositol synthesis and recovery of 5-hydroxytryptamine-responsive Ca2+ flux in blowfly salivary glands. Each salivary gland contains about 135 pmol of phosphatidylinositol. In glands prelabelled by incubation for 1 h with [32P]Pi or [3H]inositol there was a subsequent breakdown of 80% of the labelled phosphatidylinositol over a 2 h incubation period with 10 micrometer-5-hydroxytryptamine. However, there was no detectable decrease either in total phosphatidylinositol based on phosphorus analysis by chemical estimation or in the radioactivity of [32P]phosphatidylinositol in salivary glands of flies raised from the larval stage on diets containing[32P]Pi and whose phospholipids were uniformly labelled. These results suggest that the pool of phosphatidylinositol involved with Ca2+ gating is a small fraction of the total phosphatidylinositol content. Furthermore it is this small compartment that is preferentially radioactively labelled during short-term incubations with radioactively labelled precursors. In salivary glands incubated for 2 h with 10 micrometer-5-hydroxytryptamine there was a marked decrease in the flux of 45Ca2+ across the gland. After removal of the hormone, incubation of salivary glands for 1 h in the presence of 2mM-inositol, but not choline or ethanolamine, resulted in a recovery of hormone-responsive 45Ca2+ flux. Quantitative studies revealed that less than 9 pmol of phosphatidylinositol must be formed to fully restoret he 5-hydroxytryptamine-responsive 45Ca2+ flux."}
{"id": "PMID:486140", "title": "Effects of the cyanine dye 3,3'-dipropylthiocarbocyanine on mitochondrial energy conservation.", "content": "Mitochondrial respiration and oxidative phosphorylation were inhibited by the membrane potential probe 3,3'-dipropylthiocarbocyanine [diS-C3-(5)]. Evidence is presented that suggests that the dye acts as both an inhibitor of electron transport and an uncoupler of oxidative phosphorylation.", "contents": "Effects of the cyanine dye 3,3'-dipropylthiocarbocyanine on mitochondrial energy conservation. Mitochondrial respiration and oxidative phosphorylation were inhibited by the membrane potential probe 3,3'-dipropylthiocarbocyanine [diS-C3-(5)]. Evidence is presented that suggests that the dye acts as both an inhibitor of electron transport and an uncoupler of oxidative phosphorylation."}
{"id": "PMID:486141", "title": "Cytosolic location of an endo-N-acetyl-beta-D-glucosaminidase activity in rat liver and kidney.", "content": "Endo-N-acetyl-beta-D-glucosaminidase activity towards an oligomannosidic type glycoamino acid substrate was found in the soluble fraction of rat liver and kidney. No evidence for a lysosomal form of the activity was found.", "contents": "Cytosolic location of an endo-N-acetyl-beta-D-glucosaminidase activity in rat liver and kidney. Endo-N-acetyl-beta-D-glucosaminidase activity towards an oligomannosidic type glycoamino acid substrate was found in the soluble fraction of rat liver and kidney. No evidence for a lysosomal form of the activity was found."}
{"id": "PMID:486142", "title": "Effects of some chlorinated sugar derivatives on the hexose transport system of the blood/brain barrier.", "content": "The inhibition of D-glucose transport into brain by several hexose analogues has been investigated in adult anaesthetized rats. D-Glucose was transported with apparent Vmax. = 1.22 mumol/g per min, Km = 11.12 mM and Kd = 0.008 ml/g per min. 6-Chloro-6-deoxyglucose was transported with corresponding values of Vmax. = 1.33 mumol/g per min, Km = 5.5 mM and Kd = 0.0155 ml/g per min and inhibited D-glucose transport with apparent Ki = 3.01 mM. 6-Chloro-6-deoxymannose, 6-chloro-6-deoxygalactose and 6-tosyl-6-deoxygalactose also inhibited D-glucose transport, but 6-chloro-6-deoxyfructose was without effect. The results were consistent with a model for glucose transport at the blood/brain interface that involves a hydrophobic site on the transport protein at or near the 6-position of bound glucose.", "contents": "Effects of some chlorinated sugar derivatives on the hexose transport system of the blood/brain barrier. The inhibition of D-glucose transport into brain by several hexose analogues has been investigated in adult anaesthetized rats. D-Glucose was transported with apparent Vmax. = 1.22 mumol/g per min, Km = 11.12 mM and Kd = 0.008 ml/g per min. 6-Chloro-6-deoxyglucose was transported with corresponding values of Vmax. = 1.33 mumol/g per min, Km = 5.5 mM and Kd = 0.0155 ml/g per min and inhibited D-glucose transport with apparent Ki = 3.01 mM. 6-Chloro-6-deoxymannose, 6-chloro-6-deoxygalactose and 6-tosyl-6-deoxygalactose also inhibited D-glucose transport, but 6-chloro-6-deoxyfructose was without effect. The results were consistent with a model for glucose transport at the blood/brain interface that involves a hydrophobic site on the transport protein at or near the 6-position of bound glucose."}
{"id": "PMID:486143", "title": "Inhibition of renal gluconeogenesis in rats by ochratoxin.", "content": "In kidney-cortex slices from rats fed on 2.0 mg of ochratoxin A/kg per day for 2 days, gluconeogenesis from pyruvate is decreased by 26%, and renal phosphoenolpyruvate carboxykinase activity is lowered by about 55%. Gluconeogenesis from 10 mM-lactate or 20 mM-malate or -glutamine is also significantly decreased. Hepatic phosphoenolpyruvate carboxykinase is unchanged or increased, and hexokinase activity in kidney and liver remains unaffected. We conclude that ochratoxin A in vivo is an inhibitor of renal phosphoenolpyruvate carboxykinase activity, which is responsible, at least in part, for the block in renal gluconeogenesis.", "contents": "Inhibition of renal gluconeogenesis in rats by ochratoxin. In kidney-cortex slices from rats fed on 2.0 mg of ochratoxin A/kg per day for 2 days, gluconeogenesis from pyruvate is decreased by 26%, and renal phosphoenolpyruvate carboxykinase activity is lowered by about 55%. Gluconeogenesis from 10 mM-lactate or 20 mM-malate or -glutamine is also significantly decreased. Hepatic phosphoenolpyruvate carboxykinase is unchanged or increased, and hexokinase activity in kidney and liver remains unaffected. We conclude that ochratoxin A in vivo is an inhibitor of renal phosphoenolpyruvate carboxykinase activity, which is responsible, at least in part, for the block in renal gluconeogenesis."}
{"id": "PMID:486144", "title": "Loss of cell constituents from hepatocytes on centrifugation.", "content": "In studies of the metabolism of isolated hepatocytes, it is often necessary to measure the concentrations of cell constituents both in cells and medium. When hepatocytes are separated in the special tubes of Hems, Lund & Krebs (1975) (Biochem. J. 150, 47--50), they lose much glucose, urea and Na+, whereas there is no loss of K+, glutamate, aspartate and adenine nucleotides. Cell water is also lost, as measured by the distribution of 3H2O. This loss is mainly due to an exchange of cell water with the aqueous solution in the stems of the tubes through which the cells pass on centrifugation. In general, substances are lost only when the intracellular concentration is equal to, or lower than, the extracellular concentration. Probably solutes are lost because they travel with the water unidirectionally out of the cell. A loss of solute does not occur when the cells are centrifuged in conical tubes with a layer of silicone oil between the cell suspension and the deproteinizing layer. The reasons for the loss occurring in the special separation tubes are discussed.", "contents": "Loss of cell constituents from hepatocytes on centrifugation. In studies of the metabolism of isolated hepatocytes, it is often necessary to measure the concentrations of cell constituents both in cells and medium. When hepatocytes are separated in the special tubes of Hems, Lund & Krebs (1975) (Biochem. J. 150, 47--50), they lose much glucose, urea and Na+, whereas there is no loss of K+, glutamate, aspartate and adenine nucleotides. Cell water is also lost, as measured by the distribution of 3H2O. This loss is mainly due to an exchange of cell water with the aqueous solution in the stems of the tubes through which the cells pass on centrifugation. In general, substances are lost only when the intracellular concentration is equal to, or lower than, the extracellular concentration. Probably solutes are lost because they travel with the water unidirectionally out of the cell. A loss of solute does not occur when the cells are centrifuged in conical tubes with a layer of silicone oil between the cell suspension and the deproteinizing layer. The reasons for the loss occurring in the special separation tubes are discussed."}
{"id": "PMID:486145", "title": "Alterations in the rate of lipogenesis in vivo in maternal liver and adipose tissue on premature weaning of lactating rats: a possible regulatory role of prolactin.", "content": "Removal of pups for 24 h from rats at peak lactation decreased 3H2O incorporation into lipid in vivo in mammary gland by 95%, whereas it was increased in liver (77%) and adipose tissue (330%). These increases were prevented by administration of prolactin. Plasma insulin increased 3-fold on weaning and this was partially prevented by prolactin.", "contents": "Alterations in the rate of lipogenesis in vivo in maternal liver and adipose tissue on premature weaning of lactating rats: a possible regulatory role of prolactin. Removal of pups for 24 h from rats at peak lactation decreased 3H2O incorporation into lipid in vivo in mammary gland by 95%, whereas it was increased in liver (77%) and adipose tissue (330%). These increases were prevented by administration of prolactin. Plasma insulin increased 3-fold on weaning and this was partially prevented by prolactin."}
{"id": "PMID:486146", "title": "An electron-microscope study of beta-glucuronidase crystals.", "content": "beta-Glucuronidase from rat preputial glands was crystallized as thin sheets having p6 symmetry in projection with a equal 20.2nm. A filtered image was produced by Fourier methods to a resolution of 2.2 nm by averaging information from six areas. This suggests an approximately triangular molecular outline in projection, and this is taken to indicate a probable tetrahedral arrangement of the four subunits of the beta-glucuronidase molecule.", "contents": "An electron-microscope study of beta-glucuronidase crystals. beta-Glucuronidase from rat preputial glands was crystallized as thin sheets having p6 symmetry in projection with a equal 20.2nm. A filtered image was produced by Fourier methods to a resolution of 2.2 nm by averaging information from six areas. This suggests an approximately triangular molecular outline in projection, and this is taken to indicate a probable tetrahedral arrangement of the four subunits of the beta-glucuronidase molecule."}
{"id": "PMID:486147", "title": "Preparation and properties of amphipathic enzyme-polymer conjugates.", "content": "Amphipathic enzyme-polymer conjugates were prepared by reaction of hydrophilic enzymes with an anhydride polymer partially substituted with pendant hydrophobic groups. The products formed non-covalent aggregates of high molecular weight, dissociable by non-ionic detergents and urea and subject to additional aggregation at high ionic strength. Oil-in-water emulsions formed in the presence of such conjugates were shown to possess enzyme activity associated with the surface of the water-immiscible liquid. Fluorochrome labelling of conjugates showed that binding occurred at the surface of solvent droplets, and the conjugates were also found to aggregate liposomes.", "contents": "Preparation and properties of amphipathic enzyme-polymer conjugates. Amphipathic enzyme-polymer conjugates were prepared by reaction of hydrophilic enzymes with an anhydride polymer partially substituted with pendant hydrophobic groups. The products formed non-covalent aggregates of high molecular weight, dissociable by non-ionic detergents and urea and subject to additional aggregation at high ionic strength. Oil-in-water emulsions formed in the presence of such conjugates were shown to possess enzyme activity associated with the surface of the water-immiscible liquid. Fluorochrome labelling of conjugates showed that binding occurred at the surface of solvent droplets, and the conjugates were also found to aggregate liposomes."}
{"id": "PMID:486148", "title": "Some molecular properties of NAD(P)H dehydrogenase from rat liver.", "content": "NAD(P)H dehydrogenase (EC 1.6.99.2) purified from rat liver cytosol revealed three discrete bands, of mol.wts. about 27000, 18000 and 9000, when subjected to polyacrylamidegel electrophoresis in the presence of sodium dodecyl sulphate. Elution of the bands from the gel and individual re-electrophoresis on separate gels showed that the 27000-mol.wt. band yielded three bands similar to those obtained with the intact enzyme, whereas the 18000-mol.wt. band retained its characteristic mobility. Amino acid analysis of native enzyme and protein extracted from each of the three bands from sodium dodecyl sulphate/polyacrylamide gels suggests that the native enzyme is composed of two subunits and that each subunit consists of two dissimilar non-covalently bound polypeptides, so that altogether the enzyme is composed of four polypeptides, two of mol.wt. 18000 and two of mol.wt. 9000. NAD(P)H dehydrogenase was active over a wide pH range with no sharp optimum. The same K(m) value for NADH but different values for V(max.) were obtained for the enzyme purified from Sprague-Dawley and Wistar rats. In immunodiffusion, however, the enzymes from the two rat strains showed a reaction of complete identity. NAD(P)H dehydrogenase was effectively inhibited by thiol-blocking reagents, indicating that the activity is dependent on free thiol group(s). By amino acid analysis six cysteine residues were found per mol of enzyme. Guanidino-group- and amino-group-selective reagents had only moderate inactivating effects on the enzyme activity.", "contents": "Some molecular properties of NAD(P)H dehydrogenase from rat liver. NAD(P)H dehydrogenase (EC 1.6.99.2) purified from rat liver cytosol revealed three discrete bands, of mol.wts. about 27000, 18000 and 9000, when subjected to polyacrylamidegel electrophoresis in the presence of sodium dodecyl sulphate. Elution of the bands from the gel and individual re-electrophoresis on separate gels showed that the 27000-mol.wt. band yielded three bands similar to those obtained with the intact enzyme, whereas the 18000-mol.wt. band retained its characteristic mobility. Amino acid analysis of native enzyme and protein extracted from each of the three bands from sodium dodecyl sulphate/polyacrylamide gels suggests that the native enzyme is composed of two subunits and that each subunit consists of two dissimilar non-covalently bound polypeptides, so that altogether the enzyme is composed of four polypeptides, two of mol.wt. 18000 and two of mol.wt. 9000. NAD(P)H dehydrogenase was active over a wide pH range with no sharp optimum. The same K(m) value for NADH but different values for V(max.) were obtained for the enzyme purified from Sprague-Dawley and Wistar rats. In immunodiffusion, however, the enzymes from the two rat strains showed a reaction of complete identity. NAD(P)H dehydrogenase was effectively inhibited by thiol-blocking reagents, indicating that the activity is dependent on free thiol group(s). By amino acid analysis six cysteine residues were found per mol of enzyme. Guanidino-group- and amino-group-selective reagents had only moderate inactivating effects on the enzyme activity."}
{"id": "PMID:486149", "title": "A simple procedure for the isolation of a major amelogenin polypeptide component.", "content": "A simple, reproducible gel-filtration procedure for the isolation of one of the major 'J-Group' polypeptides of the bovine foetal dental-enamel matrix is described. The purified polypeptide was characterized by amino acid analysis, electrophoresis, cleavage with CNBr and N-terminal analyses. The isolated component is shown to be closely similar to an amelogenin component described by other workers.", "contents": "A simple procedure for the isolation of a major amelogenin polypeptide component. A simple, reproducible gel-filtration procedure for the isolation of one of the major 'J-Group' polypeptides of the bovine foetal dental-enamel matrix is described. The purified polypeptide was characterized by amino acid analysis, electrophoresis, cleavage with CNBr and N-terminal analyses. The isolated component is shown to be closely similar to an amelogenin component described by other workers."}
{"id": "PMID:486150", "title": "Multiple deoxyribonucleic acid polymerases from quiescent wheat embryos. Purification and characterization of three enzymes from the soluble cytoplasm and one from purified mitochondria.", "content": "Three DNA polymerases (A, B and C) have been purified from the soluble cytoplasm of ungerminated embryos. Mainly on the basis of chromatographic, template-specificity and salt-inhibition evidence, we have characterized the three enzymes. Other physico-chemical and enzymic properties are described. From purified mitochondria we have purified a DNA polymerase that behaves like DNA polymerase B on chromatographic and template-specificity criteria. Only highly purified enzyme B from the soluble cytoplasm showed an exonuclease activity able to degrade 3'- or 5'-labelled polydeoxyribonucleotides, as well as a 'proof-reading' capacity.", "contents": "Multiple deoxyribonucleic acid polymerases from quiescent wheat embryos. Purification and characterization of three enzymes from the soluble cytoplasm and one from purified mitochondria. Three DNA polymerases (A, B and C) have been purified from the soluble cytoplasm of ungerminated embryos. Mainly on the basis of chromatographic, template-specificity and salt-inhibition evidence, we have characterized the three enzymes. Other physico-chemical and enzymic properties are described. From purified mitochondria we have purified a DNA polymerase that behaves like DNA polymerase B on chromatographic and template-specificity criteria. Only highly purified enzyme B from the soluble cytoplasm showed an exonuclease activity able to degrade 3'- or 5'-labelled polydeoxyribonucleotides, as well as a 'proof-reading' capacity."}
{"id": "PMID:486151", "title": "Factors affecting the onset of deoxyribonucleic acid synthesis during wheat embryo germination. Study of the changes in DNA polymerases A, B and C and the pool of DNA precursors.", "content": "DNA synthesis starts about 12 h after water imbibition in wheat embryos. We have determined that noticeable amounts of labelled thymidine are found inside the embryo only after 6 hr of germination. DNA polymerase C from ungerminated wheat embryos decreased markedly in activity during the first hours of germination, whereas the activities of DNA polymerases A and B increased, having a maximum at about 15 h or germination. Serological evidence has suggested a clear antigenic relationship between DNA polymerases A and C. Although the pool of ATP increases rapidly after water imbibition, the increase in the pool of dNTP species was much slower.", "contents": "Factors affecting the onset of deoxyribonucleic acid synthesis during wheat embryo germination. Study of the changes in DNA polymerases A, B and C and the pool of DNA precursors. DNA synthesis starts about 12 h after water imbibition in wheat embryos. We have determined that noticeable amounts of labelled thymidine are found inside the embryo only after 6 hr of germination. DNA polymerase C from ungerminated wheat embryos decreased markedly in activity during the first hours of germination, whereas the activities of DNA polymerases A and B increased, having a maximum at about 15 h or germination. Serological evidence has suggested a clear antigenic relationship between DNA polymerases A and C. Although the pool of ATP increases rapidly after water imbibition, the increase in the pool of dNTP species was much slower."}
{"id": "PMID:486152", "title": "Chemical cross-linking of chick oviduct progesterone-receptor subunits by using a reversible bifunctional cross-linking agent.", "content": "Chick oviduct progesterone-receptor proteins were treated in cytosol with the reversible cross-linking reagent methyl 4-mercaptobutyrimidate. The product of the reaction was a 7S complex that could be detected and recovered after sucrose-density-gradient centrifugation in 0.3M-KCl. The extent of the reaction was dependent on the concentration of methyl 4-mercaptobutyrimidate and independent of the presence of bound hormone, since unlabelled receptors could also be cross-linked. The cross-linking reaction required conditions in which the cytosol 6S complex was preserved. A Stokes radius of 7.3 nm was determined by gel filtration in Agarose A-1.5 m in 0.3 M-KCl. The sedimentation coefficient, which was also determined in 0.3 M-KCl, allowed us to calculate a mol. wt. of 228,000. We were also able to cross-link partially purified receptor forms isolated by using an Agarose A-15 m column. On reduction with beta-mercaptoethanol the complex broke down to 4S monomers that were identified by DEAE-cellulose and phosphocellulose chromatography, adsorption on DNA-cellulose and gel filtration in an Agarose A-1.5 m column. In most cases, A and B receptor proteins were released in equivalent amounts, implying that the cross-linked form was an A-B complex.", "contents": "Chemical cross-linking of chick oviduct progesterone-receptor subunits by using a reversible bifunctional cross-linking agent. Chick oviduct progesterone-receptor proteins were treated in cytosol with the reversible cross-linking reagent methyl 4-mercaptobutyrimidate. The product of the reaction was a 7S complex that could be detected and recovered after sucrose-density-gradient centrifugation in 0.3M-KCl. The extent of the reaction was dependent on the concentration of methyl 4-mercaptobutyrimidate and independent of the presence of bound hormone, since unlabelled receptors could also be cross-linked. The cross-linking reaction required conditions in which the cytosol 6S complex was preserved. A Stokes radius of 7.3 nm was determined by gel filtration in Agarose A-1.5 m in 0.3 M-KCl. The sedimentation coefficient, which was also determined in 0.3 M-KCl, allowed us to calculate a mol. wt. of 228,000. We were also able to cross-link partially purified receptor forms isolated by using an Agarose A-15 m column. On reduction with beta-mercaptoethanol the complex broke down to 4S monomers that were identified by DEAE-cellulose and phosphocellulose chromatography, adsorption on DNA-cellulose and gel filtration in an Agarose A-1.5 m column. In most cases, A and B receptor proteins were released in equivalent amounts, implying that the cross-linked form was an A-B complex."}
{"id": "PMID:486153", "title": "Crystal structures of hen egg-white lysozyme complexes with gadolinium(III) and gadolinium(III)-N-acetyl-D-glucosamine.", "content": "Analysis at 0.25 nm resolution of the crystal structures of lysozyme-Gd(III) and lysozyme-Gd(III)-N-acetyl-D-glucosamine (GlcNac), prepared by diffusion methods, show that there are two main binding positions for Gd(III), one of which is close to glutamic acid-35 and the other close to aspartic acid-52. The two sites are 0.36 nm part. There is no evidence for the weak binding of Gd(III) to any of the eight other carboxy groups of lysozyme. In the presence of Gd(III), the binding of GlcNac is similar to that observed for the binding of the beta-anomer in subsite C. There are numerous small conformational changes in the protein on binding (Gd(III) and the sugar, and these have been quantified to a first approximation by real-space refinement. These changes are similar in both structures, and involve, among other small movements, shifts of one of the disulphide bridges by up to 0.05 nm. The movement of residues 70--74 observed in the binary complex of lysozyme-GlcNac [Perkins, Johnson, Machin & Phillips (1978) Biochem. J. 173-617] is not observed in the ternary complex of lysozyme-Gd(III)-GlcNac. The nature of the lysozyme-Gd(III) complex is discussed in the light of evidence from other crystallographic studies and n.m.r. solution studies. Preliminary findings for a lysozyme-Gd(III) complex prepared by co-crystallization methods are reported.", "contents": "Crystal structures of hen egg-white lysozyme complexes with gadolinium(III) and gadolinium(III)-N-acetyl-D-glucosamine. Analysis at 0.25 nm resolution of the crystal structures of lysozyme-Gd(III) and lysozyme-Gd(III)-N-acetyl-D-glucosamine (GlcNac), prepared by diffusion methods, show that there are two main binding positions for Gd(III), one of which is close to glutamic acid-35 and the other close to aspartic acid-52. The two sites are 0.36 nm part. There is no evidence for the weak binding of Gd(III) to any of the eight other carboxy groups of lysozyme. In the presence of Gd(III), the binding of GlcNac is similar to that observed for the binding of the beta-anomer in subsite C. There are numerous small conformational changes in the protein on binding (Gd(III) and the sugar, and these have been quantified to a first approximation by real-space refinement. These changes are similar in both structures, and involve, among other small movements, shifts of one of the disulphide bridges by up to 0.05 nm. The movement of residues 70--74 observed in the binary complex of lysozyme-GlcNac [Perkins, Johnson, Machin & Phillips (1978) Biochem. J. 173-617] is not observed in the ternary complex of lysozyme-Gd(III)-GlcNac. The nature of the lysozyme-Gd(III) complex is discussed in the light of evidence from other crystallographic studies and n.m.r. solution studies. Preliminary findings for a lysozyme-Gd(III) complex prepared by co-crystallization methods are reported."}
{"id": "PMID:486154", "title": "Immunochemically identical hydrophilic and amphiphilic forms of the bovine adrenomedullary dopamine beta-hydroxylase.", "content": "By means of a monospecific antibody, dopamine beta-hydroxylase was monitored immunoelectrophoretically in various extracts of chromaffin granules. Approximately one-third of the dopamine beta-hydroxylase present was located in the membrane fraction and could only be liberated with detergent. The dopamine beta-hydroxylases of the buffer and membrane fractions were antigenically identical, but differed in their amphiphilicity, as demonstrated by the change in precipitation patterns on removal of Triton X-100 from the gel, on charge-shift crossed immunoelectrophoresis and on crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose. Furthermore, immunoelectrophoretic analysis in the presence of Triton X-100 plus the cationic detergent cetyltrimethylammonium bromide indicates additional heterogeneity of the membrane-bound dopamine-beta-hydroxylase. By limited proteolysis with chymotrypsin and thermolysin the amphiphilic form could be convered into its hydrophilic counterpart.", "contents": "Immunochemically identical hydrophilic and amphiphilic forms of the bovine adrenomedullary dopamine beta-hydroxylase. By means of a monospecific antibody, dopamine beta-hydroxylase was monitored immunoelectrophoretically in various extracts of chromaffin granules. Approximately one-third of the dopamine beta-hydroxylase present was located in the membrane fraction and could only be liberated with detergent. The dopamine beta-hydroxylases of the buffer and membrane fractions were antigenically identical, but differed in their amphiphilicity, as demonstrated by the change in precipitation patterns on removal of Triton X-100 from the gel, on charge-shift crossed immunoelectrophoresis and on crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose. Furthermore, immunoelectrophoretic analysis in the presence of Triton X-100 plus the cationic detergent cetyltrimethylammonium bromide indicates additional heterogeneity of the membrane-bound dopamine-beta-hydroxylase. By limited proteolysis with chymotrypsin and thermolysin the amphiphilic form could be convered into its hydrophilic counterpart."}
{"id": "PMID:486155", "title": "On the molecular-weight-dependence of the anticoagulant activity of heparin.", "content": "The inactivation of thrombin and factor Xa by antithrombin was determined in the presence of heparin fractions of different molecular weights and with high affinity for antithrombin. The ability to potentiate the inactivation of either coagulation factor increased with increasing length of the polysaccharide chain.", "contents": "On the molecular-weight-dependence of the anticoagulant activity of heparin. The inactivation of thrombin and factor Xa by antithrombin was determined in the presence of heparin fractions of different molecular weights and with high affinity for antithrombin. The ability to potentiate the inactivation of either coagulation factor increased with increasing length of the polysaccharide chain."}
{"id": "PMID:486156", "title": "Inhibition of human alkaline phosphatases by vanadate.", "content": "Orthovanadate was shown to be a potent competitive inhibitor (Ki less than 1 microM) of purified alkaline phosphatase from human liver, intestine of kidney. Inhibition was reversed and full enzymic activity restored in the presence of 1mM-adrenaline. Phosphate and vanadate competed for the same binding site on the enzyme.", "contents": "Inhibition of human alkaline phosphatases by vanadate. Orthovanadate was shown to be a potent competitive inhibitor (Ki less than 1 microM) of purified alkaline phosphatase from human liver, intestine of kidney. Inhibition was reversed and full enzymic activity restored in the presence of 1mM-adrenaline. Phosphate and vanadate competed for the same binding site on the enzyme."}
{"id": "PMID:486157", "title": "Dansylation of human serum albumin in the study of the primary binding sites of bilirubin and L-tryptophan.", "content": "Binding of bilirubin and of L-tryptophan to dansylated albumins was investigated. Dansylation of less than one lysine residue per molecule of albumin did not affect the bilirubin binding, but decreased the L-tryptophan binding, indicating that dansylation had taken place in or near the l-tryptophan-binding site. Native albumin and albumin-bilirubin 1:1 complex showed the same affinity for L-tryptophan. The results indicate that, although L-tryptophan and bilirubin are bound in the same region, perhaps in a common cavity of the albumin molecule, such a cavity is sufficiently large to contain both ligands.", "contents": "Dansylation of human serum albumin in the study of the primary binding sites of bilirubin and L-tryptophan. Binding of bilirubin and of L-tryptophan to dansylated albumins was investigated. Dansylation of less than one lysine residue per molecule of albumin did not affect the bilirubin binding, but decreased the L-tryptophan binding, indicating that dansylation had taken place in or near the l-tryptophan-binding site. Native albumin and albumin-bilirubin 1:1 complex showed the same affinity for L-tryptophan. The results indicate that, although L-tryptophan and bilirubin are bound in the same region, perhaps in a common cavity of the albumin molecule, such a cavity is sufficiently large to contain both ligands."}
{"id": "PMID:486158", "title": "A comment on the 'jack-knife' technique for analysing enzyme-kinetic data.", "content": "The use of the 'jack-knife' technique in the analysis of enzyme-kinetic data [Cornish-Bowden & Wong (1978] Biochem. J. 175, 969--976) is examined. The method can give parameter estimates that appear to be incorrect.", "contents": "A comment on the 'jack-knife' technique for analysing enzyme-kinetic data. The use of the 'jack-knife' technique in the analysis of enzyme-kinetic data [Cornish-Bowden & Wong (1978] Biochem. J. 175, 969--976) is examined. The method can give parameter estimates that appear to be incorrect."}
{"id": "PMID:486159", "title": "Glutathione S-transferases in earthworms (Lumbricidae).", "content": "Glutathione S-transferase activity (EC 2.5.1.18) was demonstrated in six species of earthworms of the family Lumbricidae: Eisenia foetida, Lumbricus terrestris, Lumbricus rebellus, Allolobophora longa, Allolobophora caliginosa and Allolobophora chlorotica. Considerable activity was obtained with 1-chlorl-2,4-dinitrobenzene and low activity with 3,4-dichloro-1-nitrobenzene, but no enzymic reaction was detectable with sulphobromophthalein 1,2-epoxy-3-(p-nitrophenoxy)propane of trans-4-phenylbut-3-en-2-one as substrates. Enzyme prepartations from L. rubellus and A. longa were the most active, whereas A. chlorotica gave the lowest activity. The ratio of the activities obtained with 1-chloro-2,4-dinitrobenzene and 3,4-cichloro-1-nitrobenzene was very different in the various species, but no phylogenetic pattern was evident. Isoelectric focusing gave rise to various activity peaks as measured with 1-chloro-2,4-dinitrobenzene as a substrate, and the activity profiles of the species examined appeared to follow a taxonomic pattern. The activity of Allolobophora had the highest peak in the alkaline region, whereas that of Lumbricus had the highest peak in the acid region. Eisenia showed a very complex activity profile, with the highest peak ne pH 7. As determined by an enzymic assay, all the species contained glutathione, on an average about 0.5 mumol/g wet wt. Conjugation with glutathione catalysed by glutathione S-transferases may consequently be an important detoxification mechanism in earthworms.", "contents": "Glutathione S-transferases in earthworms (Lumbricidae). Glutathione S-transferase activity (EC 2.5.1.18) was demonstrated in six species of earthworms of the family Lumbricidae: Eisenia foetida, Lumbricus terrestris, Lumbricus rebellus, Allolobophora longa, Allolobophora caliginosa and Allolobophora chlorotica. Considerable activity was obtained with 1-chlorl-2,4-dinitrobenzene and low activity with 3,4-dichloro-1-nitrobenzene, but no enzymic reaction was detectable with sulphobromophthalein 1,2-epoxy-3-(p-nitrophenoxy)propane of trans-4-phenylbut-3-en-2-one as substrates. Enzyme prepartations from L. rubellus and A. longa were the most active, whereas A. chlorotica gave the lowest activity. The ratio of the activities obtained with 1-chloro-2,4-dinitrobenzene and 3,4-cichloro-1-nitrobenzene was very different in the various species, but no phylogenetic pattern was evident. Isoelectric focusing gave rise to various activity peaks as measured with 1-chloro-2,4-dinitrobenzene as a substrate, and the activity profiles of the species examined appeared to follow a taxonomic pattern. The activity of Allolobophora had the highest peak in the alkaline region, whereas that of Lumbricus had the highest peak in the acid region. Eisenia showed a very complex activity profile, with the highest peak ne pH 7. As determined by an enzymic assay, all the species contained glutathione, on an average about 0.5 mumol/g wet wt. Conjugation with glutathione catalysed by glutathione S-transferases may consequently be an important detoxification mechanism in earthworms."}
{"id": "PMID:486160", "title": "The interaction of calcium ions with glycocholate micelles in aqueous solution.", "content": "The formation of soluble complexes of Ca2+ ions and glycocholate has been demonstrated. The dissociation constant is 26 nmol/litre and a maximum of 2 Ca2+ ions are bound to each glycocholate micelle. The formation of this complex is shown to be reversible. Binding is increased by the introduction of phosphatidylcholine into the micelle; it is decreased by a decrease in pH and by increased counter-ion concentration. The biological significance of these effects is discussed.", "contents": "The interaction of calcium ions with glycocholate micelles in aqueous solution. The formation of soluble complexes of Ca2+ ions and glycocholate has been demonstrated. The dissociation constant is 26 nmol/litre and a maximum of 2 Ca2+ ions are bound to each glycocholate micelle. The formation of this complex is shown to be reversible. Binding is increased by the introduction of phosphatidylcholine into the micelle; it is decreased by a decrease in pH and by increased counter-ion concentration. The biological significance of these effects is discussed."}
{"id": "PMID:486161", "title": "The vectorial orientation of human monoamine oxidase in the mitochondrial outer membrane.", "content": "1. The localization of monoamine oxidase in the mitochondrial outer membrane was studied in preparations of human liver mitochondrial and brain-cortex non-synaptosomal and synaptosomal mitochondria. 2. Immunochemical accessibility in iso-osmotic and hypo-osmotic mitochondrial preparations was used to localize the enzyme. 3. It was shown that the immunochemically accessible tyramine-oxidizing activity was distributed approximately equally on both surfaces of the membrane in human liver and brain-cortex non-synaptosomal mitochondria. However, the immunochemically accessible beta-phenethylamine-oxidizing activity was situated predominantly on the outer surface, and the immunochemically accessible 5-hydroxytryptamine-oxidizing activity was situated predominantly on the inner surface of the mitochondrial outer membrane in liver and brain-cortex non-synaptosomal mitochondrial preparations. 4. Considerable variation in the distribution of the enzyme in preparations of synaptosomal mitochondria was seen. 5. The simplest model consistent with our observations is that, in liver and brain-cortex non-synaptosomal mitochondria, the tyramine-oxidizing activity is distributed on both sides of the mitochondrial outer membrane, the beta-phenethylamine-oxidizing activity is located on the outer surface of the outer membrane and the 5-hydroxytryptamine-oxidizing activity is located on the inner surface of the mitochondria outer membrane.", "contents": "The vectorial orientation of human monoamine oxidase in the mitochondrial outer membrane. 1. The localization of monoamine oxidase in the mitochondrial outer membrane was studied in preparations of human liver mitochondrial and brain-cortex non-synaptosomal and synaptosomal mitochondria. 2. Immunochemical accessibility in iso-osmotic and hypo-osmotic mitochondrial preparations was used to localize the enzyme. 3. It was shown that the immunochemically accessible tyramine-oxidizing activity was distributed approximately equally on both surfaces of the membrane in human liver and brain-cortex non-synaptosomal mitochondria. However, the immunochemically accessible beta-phenethylamine-oxidizing activity was situated predominantly on the outer surface, and the immunochemically accessible 5-hydroxytryptamine-oxidizing activity was situated predominantly on the inner surface of the mitochondrial outer membrane in liver and brain-cortex non-synaptosomal mitochondrial preparations. 4. Considerable variation in the distribution of the enzyme in preparations of synaptosomal mitochondria was seen. 5. The simplest model consistent with our observations is that, in liver and brain-cortex non-synaptosomal mitochondria, the tyramine-oxidizing activity is distributed on both sides of the mitochondrial outer membrane, the beta-phenethylamine-oxidizing activity is located on the outer surface of the outer membrane and the 5-hydroxytryptamine-oxidizing activity is located on the inner surface of the mitochondria outer membrane."}
{"id": "PMID:486162", "title": "Metabolism of rabbit skin collagen. Differences in the apparent turnover rates of type-I- and type-III-collagen precursors determined by constant intravenous infusion of labelled amino acids.", "content": "Growing rabbits were infused for up to 10 h with labelled proline, tyrosine and leucine to achieve plateau conditions within body free pools, for [3H]proline infusion, blood free-proline specific radioactivity remained constant after about 1 h. For individual animals, type-I- and type-III-collagen precursors were isolated by precipitation with (NH4)2SO4 and DEAE-cellulose chromatography. Experiments where 3H- and 14C-labelled proline and tyrosine were infused concurrently for different periods of time showed that type I procollagen reached plateau specific radioactivity within 3 h and 90% of the plateau value after 2 h infusion, corresponding to a calculated apparent t 1/2 of less than 26 min. Plateau values for type I procollagen were taken as precursor amino acid pool specific radioactivities. The type-III-collagen-precursor fractions consistently showed lower rates of label incorporation and, by assuming that both type I and type III collagens are synthesized from the same amino acid pools, kinetic analysis revealed an apparent t 1/2 for the isolated type-III-collagen precursors of 3.9 h. For proline, there were large variations between animals in the ratio between the precursor pool for collagen synthesis and the skin homogenate free pool (0.31 +/- 0.13, mean +/- S.D.), so that collagen-synthesis rates based solely on total tissue free-pool values for proline are subject to large and inconsistent errors.", "contents": "Metabolism of rabbit skin collagen. Differences in the apparent turnover rates of type-I- and type-III-collagen precursors determined by constant intravenous infusion of labelled amino acids. Growing rabbits were infused for up to 10 h with labelled proline, tyrosine and leucine to achieve plateau conditions within body free pools, for [3H]proline infusion, blood free-proline specific radioactivity remained constant after about 1 h. For individual animals, type-I- and type-III-collagen precursors were isolated by precipitation with (NH4)2SO4 and DEAE-cellulose chromatography. Experiments where 3H- and 14C-labelled proline and tyrosine were infused concurrently for different periods of time showed that type I procollagen reached plateau specific radioactivity within 3 h and 90% of the plateau value after 2 h infusion, corresponding to a calculated apparent t 1/2 of less than 26 min. Plateau values for type I procollagen were taken as precursor amino acid pool specific radioactivities. The type-III-collagen-precursor fractions consistently showed lower rates of label incorporation and, by assuming that both type I and type III collagens are synthesized from the same amino acid pools, kinetic analysis revealed an apparent t 1/2 for the isolated type-III-collagen precursors of 3.9 h. For proline, there were large variations between animals in the ratio between the precursor pool for collagen synthesis and the skin homogenate free pool (0.31 +/- 0.13, mean +/- S.D.), so that collagen-synthesis rates based solely on total tissue free-pool values for proline are subject to large and inconsistent errors."}
{"id": "PMID:486163", "title": "Lipoprotein lipase activity of rat cardiac muscle. The intracellular distribution of the enzyme between fractions prepared from cardiac muscle and cells isolated from the hearts of fed and starved animals.", "content": "1. Subcellular fractions, characterized by using morphological, compositional and enzymic markers, were prepared from rat heart tissue and cells isolated from the hearts of fed and 24 h-starved rats. 2. The lipoprotein lipase activity of fractions from whole tissue and isolated cells was determined in either fresh fractions or in acetone/diethyl ether powders of the fractions. 3. Lipoprotein lipase activity was present in all the fractions from tissue and cells, but was found to be of highest relative specific activity in the microsomal () fractions. 4. In fractions prepared from the isolated cells of hearts from starved rats the proportion of the total lipoprotein lipase present and its relative specific activity in the microsomal fraction were greater than in the equivalent fractions from fed animals. 5. The enhancement of lipoprotein lipase activity as a result of the acetone/diethyl ether powder preparation of fractions was most extensive in the microsomal fractions. 6. Investigation of the microsomal fraction showed that the lipoprotein lipase activity present was in two pools, one of which was within endoplasmic-reticulum vesicles. 7. The observations were consistent with the possibility that the cardiac-muscle cell could be the origin of the lipoprotein lipase activity functional in triacylglycerol uptake by the heart.", "contents": "Lipoprotein lipase activity of rat cardiac muscle. The intracellular distribution of the enzyme between fractions prepared from cardiac muscle and cells isolated from the hearts of fed and starved animals. 1. Subcellular fractions, characterized by using morphological, compositional and enzymic markers, were prepared from rat heart tissue and cells isolated from the hearts of fed and 24 h-starved rats. 2. The lipoprotein lipase activity of fractions from whole tissue and isolated cells was determined in either fresh fractions or in acetone/diethyl ether powders of the fractions. 3. Lipoprotein lipase activity was present in all the fractions from tissue and cells, but was found to be of highest relative specific activity in the microsomal () fractions. 4. In fractions prepared from the isolated cells of hearts from starved rats the proportion of the total lipoprotein lipase present and its relative specific activity in the microsomal fraction were greater than in the equivalent fractions from fed animals. 5. The enhancement of lipoprotein lipase activity as a result of the acetone/diethyl ether powder preparation of fractions was most extensive in the microsomal fractions. 6. Investigation of the microsomal fraction showed that the lipoprotein lipase activity present was in two pools, one of which was within endoplasmic-reticulum vesicles. 7. The observations were consistent with the possibility that the cardiac-muscle cell could be the origin of the lipoprotein lipase activity functional in triacylglycerol uptake by the heart."}
{"id": "PMID:486164", "title": "Flavin-dependent substrate photo-oxidation as a chemical model of dehydrogenase action.", "content": "As a model of flavin-dependent biological dehydrogenation, flavin-sensitized photodehydrogenation and photodecarboxylation were studied by variation of substrate, flavin, pH and solvent. Evidence for the following rules is given. (1) When the reactive site of a photosubstrate is an alpha-carbon atom of the type CH-CO2-, decarboxylation is preferred over dehydrogenation, whereas the reverse is true for the neutral CH-CO2H. (2) Consequently these reactions do not exhibit a measurable isotope effect with C2H-CO2-, in contrast with the findings by Penzer, Radda, Taylor & Taylor [(1970) Vitam. Horm. (N.Y.) 28, 441--466], which could not be reproduced. When the substate does not contain a carboxylate group, isotope effects occur, in verification of previous reports, e.g. for benzyl alcohol C6H5-C2H20H. (3) The mechanism of flavin-sensitized substrate photodecarboxylation is assumed to consist in a primary carbanion fixation at the flavin nucleus (position 4a, 5 or 8) with concomitant liberation of CO2. This step is followed by rapid fragmentation of the adduct CH-Fl-red., provided that the substrate contains a functional and electron-donating group X, e.g. X = OH, OCH3 or NH2 (but not NH3+ !) in X CH-CO2-. (4) The minimal requirement for flavin-sensitized C-H dehydrogenation is the presence of a hydroxyl group. For example, methanol as substrate and solvent is dehydrogenated at pH sufficiently alkaline for detection of the presence of the active species CH3O-, whereas at more acidic pH substrate dehydrogenation is competing with flavin autophotolysis, which depends on the substituents in the flavin nucleus.", "contents": "Flavin-dependent substrate photo-oxidation as a chemical model of dehydrogenase action. As a model of flavin-dependent biological dehydrogenation, flavin-sensitized photodehydrogenation and photodecarboxylation were studied by variation of substrate, flavin, pH and solvent. Evidence for the following rules is given. (1) When the reactive site of a photosubstrate is an alpha-carbon atom of the type CH-CO2-, decarboxylation is preferred over dehydrogenation, whereas the reverse is true for the neutral CH-CO2H. (2) Consequently these reactions do not exhibit a measurable isotope effect with C2H-CO2-, in contrast with the findings by Penzer, Radda, Taylor & Taylor [(1970) Vitam. Horm. (N.Y.) 28, 441--466], which could not be reproduced. When the substate does not contain a carboxylate group, isotope effects occur, in verification of previous reports, e.g. for benzyl alcohol C6H5-C2H20H. (3) The mechanism of flavin-sensitized substrate photodecarboxylation is assumed to consist in a primary carbanion fixation at the flavin nucleus (position 4a, 5 or 8) with concomitant liberation of CO2. This step is followed by rapid fragmentation of the adduct CH-Fl-red., provided that the substrate contains a functional and electron-donating group X, e.g. X = OH, OCH3 or NH2 (but not NH3+ !) in X CH-CO2-. (4) The minimal requirement for flavin-sensitized C-H dehydrogenation is the presence of a hydroxyl group. For example, methanol as substrate and solvent is dehydrogenated at pH sufficiently alkaline for detection of the presence of the active species CH3O-, whereas at more acidic pH substrate dehydrogenation is competing with flavin autophotolysis, which depends on the substituents in the flavin nucleus."}
{"id": "PMID:486218", "title": "Lymphocyte depletion by continuous flow cell centrifugation in rheumatoid arthritis: clinical effects.", "content": "Four patients with active, severe rheumatoid arthritis were subjected to the removal of circulating lymphocytes (lymphapheresis) for a 6-week period by use of a continuous flow cell centrifuge. Repetitive venous access was gained through forearm arteriovenous fistulae. In all patients the Ritchie-Camp articular index declined rapidly in the first week and more slowly therafter for a total mean drop of 61% below the initial value. The index continued to decline after lymphapheresis but exacerbation of disease activity developed at an average of 19 weeks. No significant untoward effects of the procedure were observed; occasional transfusions were required to compensate for the small, unavoidable erythrocyte loss.", "contents": "Lymphocyte depletion by continuous flow cell centrifugation in rheumatoid arthritis: clinical effects. Four patients with active, severe rheumatoid arthritis were subjected to the removal of circulating lymphocytes (lymphapheresis) for a 6-week period by use of a continuous flow cell centrifuge. Repetitive venous access was gained through forearm arteriovenous fistulae. In all patients the Ritchie-Camp articular index declined rapidly in the first week and more slowly therafter for a total mean drop of 61% below the initial value. The index continued to decline after lymphapheresis but exacerbation of disease activity developed at an average of 19 weeks. No significant untoward effects of the procedure were observed; occasional transfusions were required to compensate for the small, unavoidable erythrocyte loss."}
{"id": "PMID:486220", "title": "Cardiac involvement in polymyositis: a clinicopathologic study of 20 autopsied patients.", "content": "Cardiac involvement in polymyositis was investigated in 20 autopsied cases. Clinically, 13 of 18 patients had abnormal electrocardiograms, and 9 of the 20 patients had previous evidence of congestive heart failure. Histologically documented myocarditis was detected in 6 patients (4 with congestive heart failure and 2 without), 4 of whom also had small vessel disease of the myocardium. Patients with polymyositis may have a cardiopathy in the absence of overt myocardial inflammatory disease.", "contents": "Cardiac involvement in polymyositis: a clinicopathologic study of 20 autopsied patients. Cardiac involvement in polymyositis was investigated in 20 autopsied cases. Clinically, 13 of 18 patients had abnormal electrocardiograms, and 9 of the 20 patients had previous evidence of congestive heart failure. Histologically documented myocarditis was detected in 6 patients (4 with congestive heart failure and 2 without), 4 of whom also had small vessel disease of the myocardium. Patients with polymyositis may have a cardiopathy in the absence of overt myocardial inflammatory disease."}
{"id": "PMID:486221", "title": "Articular cartilage preservation and storage. II. Mechanical indentation testing of viable, stored articular cartilage.", "content": "Mature rabbit articular cartilage in the form of distal femoral condyles, composite osteoarticular structures, were incubated in the presence of alpha-tocopherol (200 micrograms/ml) over a period of time. Indentation testing and 35S uptake indicate preservation of sustained load carrying capacity and viability, respectively, in the presence of alpha-tocopherol for up to 30 days in organ culture. Condylar cartilage stored in the absence of alpha-tocopherol as well as frozen cartilage demonstrated progressive inability to resist sustained loading over time. Nonoptimal synthetic function apparently occurred in these latter two groups when compared to alpha-tocopherol stored material.", "contents": "Articular cartilage preservation and storage. II. Mechanical indentation testing of viable, stored articular cartilage. Mature rabbit articular cartilage in the form of distal femoral condyles, composite osteoarticular structures, were incubated in the presence of alpha-tocopherol (200 micrograms/ml) over a period of time. Indentation testing and 35S uptake indicate preservation of sustained load carrying capacity and viability, respectively, in the presence of alpha-tocopherol for up to 30 days in organ culture. Condylar cartilage stored in the absence of alpha-tocopherol as well as frozen cartilage demonstrated progressive inability to resist sustained loading over time. Nonoptimal synthetic function apparently occurred in these latter two groups when compared to alpha-tocopherol stored material."}
{"id": "PMID:486222", "title": "In vitro hexosamine depletion of intact articular cartilage by E-prostaglandins: prevention by chloroquine.", "content": "Short-term incubations of intact canine articular cartilage slices with prostaglandins E1 and E2 caused significant losses of hexosamine from cartilage matrix compared to controls. Chloroquine, an inhibitor of DNA primer, prevented this prostaglandin-induced hexosamine depletion. These data suggest that E-prostaglandins may degrade articular cartilage directly through DNA-dependent RNA synthesis of cathepsin-like proteases. Catabolism of articular cartilage probably involves degradation of existing matrix in addition to inhibition of synthesis.", "contents": "In vitro hexosamine depletion of intact articular cartilage by E-prostaglandins: prevention by chloroquine. Short-term incubations of intact canine articular cartilage slices with prostaglandins E1 and E2 caused significant losses of hexosamine from cartilage matrix compared to controls. Chloroquine, an inhibitor of DNA primer, prevented this prostaglandin-induced hexosamine depletion. These data suggest that E-prostaglandins may degrade articular cartilage directly through DNA-dependent RNA synthesis of cathepsin-like proteases. Catabolism of articular cartilage probably involves degradation of existing matrix in addition to inhibition of synthesis."}
{"id": "PMID:486224", "title": "Evaluation of smoking-induced effects on sympathetic, hemodynamic and metabolic variables with respect to plasma nicotine and COHb levels.", "content": "The effect of smoking cigarettes containing 1.5 mg and 0.08 mg nicotine per cigarette and of sham-smoking was studied in six healthy habitual smokers. Levels of carboxyhemoglobin (COHb) and plasma nicotine were measured simultaneously with hemodynamic variables, such as heart rate and blood pressure, and with the metabolic parameters, plasma DBH, cortisol, blood glucose, lactate and free fatty acids. All variables, with the exception of COHb are dose related to plasma nicotine levels. Blood pressure, heart rate and lactate show simultaneous peaks together with maximal nicotine levels, while DBH and cortisol, blood glucose and free fatty acids show a delayed reaction compared to nicotine concentrations. No effects of COHb, even with levels up to 5.6 +/- 0.5% have been observed on the variables investigated. These results demonstrate, that it is nicotine which induces considerable hemodynamic and metabolic alterations after smoking.", "contents": "Evaluation of smoking-induced effects on sympathetic, hemodynamic and metabolic variables with respect to plasma nicotine and COHb levels. The effect of smoking cigarettes containing 1.5 mg and 0.08 mg nicotine per cigarette and of sham-smoking was studied in six healthy habitual smokers. Levels of carboxyhemoglobin (COHb) and plasma nicotine were measured simultaneously with hemodynamic variables, such as heart rate and blood pressure, and with the metabolic parameters, plasma DBH, cortisol, blood glucose, lactate and free fatty acids. All variables, with the exception of COHb are dose related to plasma nicotine levels. Blood pressure, heart rate and lactate show simultaneous peaks together with maximal nicotine levels, while DBH and cortisol, blood glucose and free fatty acids show a delayed reaction compared to nicotine concentrations. No effects of COHb, even with levels up to 5.6 +/- 0.5% have been observed on the variables investigated. These results demonstrate, that it is nicotine which induces considerable hemodynamic and metabolic alterations after smoking."}
{"id": "PMID:486226", "title": "Blood levels, tissue distribution and the duration of action in rats of ciprofibrate, a new hypolipidemic agent.", "content": "The blood levels, distribution and duration of action of ciprofibrate, an orally active hypolipidemic agent, was investigated in rats. Serum concentrations of 30 micrograms of ciprofibrate/ml are associated with significant reductions in both serum cholesterol and triglycerides in rats on a hyperlipidemic diet. Increasing the plasma concentrations of ciprofibrate to 69 micrograms/ml resulted in only a modest incremental reduction in serum lipids. The distribution of radioactivity from [14C]ciprofibrate within rat tissues was not affected by prior treatment for 14 days with ciprofibrate at either 1.5 or 3.0 mg/kg/day. Varying the dosage regimen of ciprofibrate at 30 mg/kg, with medication at intervals of one, 2 or 3 days resulted in similar peak plasma levels of about 300 micrograms/ml, 4 h after medication. The half-life of ciprofibrate, during the terminal disposition phase, was about 82 h. Levels of serum cholesterol remained suppressed up to 3 days after medication with ciprofibrate was discontinued; triglyceride levels returned to control values more slowly.", "contents": "Blood levels, tissue distribution and the duration of action in rats of ciprofibrate, a new hypolipidemic agent. The blood levels, distribution and duration of action of ciprofibrate, an orally active hypolipidemic agent, was investigated in rats. Serum concentrations of 30 micrograms of ciprofibrate/ml are associated with significant reductions in both serum cholesterol and triglycerides in rats on a hyperlipidemic diet. Increasing the plasma concentrations of ciprofibrate to 69 micrograms/ml resulted in only a modest incremental reduction in serum lipids. The distribution of radioactivity from [14C]ciprofibrate within rat tissues was not affected by prior treatment for 14 days with ciprofibrate at either 1.5 or 3.0 mg/kg/day. Varying the dosage regimen of ciprofibrate at 30 mg/kg, with medication at intervals of one, 2 or 3 days resulted in similar peak plasma levels of about 300 micrograms/ml, 4 h after medication. The half-life of ciprofibrate, during the terminal disposition phase, was about 82 h. Levels of serum cholesterol remained suppressed up to 3 days after medication with ciprofibrate was discontinued; triglyceride levels returned to control values more slowly."}
{"id": "PMID:486225", "title": "Reduction of serum cholesterol in two patients with homozygous familial hypercholesterolemia by dichloroacetate.", "content": "Dichloroacetate is known to reduce plasma cholesterol and triglyceride in patients with Fredrickson Types IIb or IV hyperlipoproteinemia. We now report the effects of chronic, oral dichloroacetate administration (as the sodium salt) in two patients with severe homozygous familial hypercholesterolemia. Dichloroacetate markedly reduced serum total and low density lipoprotein cholesterol levels and lowered the low density lipoprotein to high density lipoprotein cholesterol ratio. One patient developed a polyneuropathy while receiving dichloroacetate which resolved following discontinuation of the drug. Because of its apparent toxicity, dichloroacetate cannot be recommended for chronic oral use. Investigation of the mechanism of its lipid-lowering effect, however, may provide insight into the pathogenesis and treatment of hypercholesterolemic disorders.", "contents": "Reduction of serum cholesterol in two patients with homozygous familial hypercholesterolemia by dichloroacetate. Dichloroacetate is known to reduce plasma cholesterol and triglyceride in patients with Fredrickson Types IIb or IV hyperlipoproteinemia. We now report the effects of chronic, oral dichloroacetate administration (as the sodium salt) in two patients with severe homozygous familial hypercholesterolemia. Dichloroacetate markedly reduced serum total and low density lipoprotein cholesterol levels and lowered the low density lipoprotein to high density lipoprotein cholesterol ratio. One patient developed a polyneuropathy while receiving dichloroacetate which resolved following discontinuation of the drug. Because of its apparent toxicity, dichloroacetate cannot be recommended for chronic oral use. Investigation of the mechanism of its lipid-lowering effect, however, may provide insight into the pathogenesis and treatment of hypercholesterolemic disorders."}
{"id": "PMID:486227", "title": "Effect of various isoflavones on lipid levels in triton-treated rats.", "content": "The isoflavone content of some commonly used legumes was determined. In Triton-WR1339-induced hyperlipidemia in male albino rats, biochanin A, formononetin and pratensein showed hypolipidemic activity, while diadzein did not, when these isoflavones were administered as individual compounds by gastric intubation.", "contents": "Effect of various isoflavones on lipid levels in triton-treated rats. The isoflavone content of some commonly used legumes was determined. In Triton-WR1339-induced hyperlipidemia in male albino rats, biochanin A, formononetin and pratensein showed hypolipidemic activity, while diadzein did not, when these isoflavones were administered as individual compounds by gastric intubation."}
{"id": "PMID:486229", "title": "Renal papillary necrosis and pyelonephritis accompanying fenoprofen therapy.", "content": "Renal papillary necrosis occurred after fenoprofen calcium administration in a patient with systemic lupus erythematosus and urinary tract infection. Possible mechanisms of renal damage may be hypersensitivity, decreased blood flow, and decreased production of a prostaglandin E-like substance.", "contents": "Renal papillary necrosis and pyelonephritis accompanying fenoprofen therapy. Renal papillary necrosis occurred after fenoprofen calcium administration in a patient with systemic lupus erythematosus and urinary tract infection. Possible mechanisms of renal damage may be hypersensitivity, decreased blood flow, and decreased production of a prostaglandin E-like substance."}
{"id": "PMID:486232", "title": "Subcellular distribution of radio-labelled iron during intestinal absorption in guinea-pig enterocytes with special reference to the mitochondrial localization of the iron.", "content": "1. 59Fe-labelled ferric chloride was introduced into tied loops of guinea-pig jejunum. 2. After 5--30 min the loop was removed, the enterocytes were isolated, homogenized and subjected to analytical subcellular fractionation. 3. Uptake of 59Fe was extremely rapid and after 5 min 45% of the radioactivity sedimented in the mitochondrial fraction. 4. Density gradient centrifugation indicated that approximately 80% of this radioactivity was associated with the mitochondria themselves; the remainder was in brush-border fragments. 5. Selective disruption of the mitochondria demonstrates that the iron is localized to the matrix and the inner membrane, indicating transport of the absorbed iron into the organelle. 6. It is suggested that mitochondria are actively implicated at an early stage in the intestinal transport of iron.", "contents": "Subcellular distribution of radio-labelled iron during intestinal absorption in guinea-pig enterocytes with special reference to the mitochondrial localization of the iron. 1. 59Fe-labelled ferric chloride was introduced into tied loops of guinea-pig jejunum. 2. After 5--30 min the loop was removed, the enterocytes were isolated, homogenized and subjected to analytical subcellular fractionation. 3. Uptake of 59Fe was extremely rapid and after 5 min 45% of the radioactivity sedimented in the mitochondrial fraction. 4. Density gradient centrifugation indicated that approximately 80% of this radioactivity was associated with the mitochondria themselves; the remainder was in brush-border fragments. 5. Selective disruption of the mitochondria demonstrates that the iron is localized to the matrix and the inner membrane, indicating transport of the absorbed iron into the organelle. 6. It is suggested that mitochondria are actively implicated at an early stage in the intestinal transport of iron."}
{"id": "PMID:486233", "title": "Effects of 'elemental' diets on absorptive and enzymic activities and on 5-fluorouracil toxicity in rat small intestine.", "content": "1. Water absorption and cytoplasmic dipeptidase activities were determined in isolated rat small intestine after animals had fed on various 'elemental' diets or a standard rat diet and after a single injection of 5-fluorouracil. 2. Vivonex diets were associated with a decrease in dry weight of the small intestine and an increase in glucose and water absorption in vitro. 3. The nature of the dietary nitrogen profoundly affects the activities of intestinal peptidases. 4. Water absorption was severly depressed 3 days after 5-fluorouracil administration: the extent of depression was independent of the diet. Thus, any ameliorative effects of 'elemental' diets on 5-fluorouracil toxicity are unlikely to be mediated by direct protection of intestinal absorption.", "contents": "Effects of 'elemental' diets on absorptive and enzymic activities and on 5-fluorouracil toxicity in rat small intestine. 1. Water absorption and cytoplasmic dipeptidase activities were determined in isolated rat small intestine after animals had fed on various 'elemental' diets or a standard rat diet and after a single injection of 5-fluorouracil. 2. Vivonex diets were associated with a decrease in dry weight of the small intestine and an increase in glucose and water absorption in vitro. 3. The nature of the dietary nitrogen profoundly affects the activities of intestinal peptidases. 4. Water absorption was severly depressed 3 days after 5-fluorouracil administration: the extent of depression was independent of the diet. Thus, any ameliorative effects of 'elemental' diets on 5-fluorouracil toxicity are unlikely to be mediated by direct protection of intestinal absorption."}
{"id": "PMID:486238", "title": "Dependency upon salbutamol inhalers.", "content": "A case of dependency on salbutamol inhalers has been described. This has been discussed in relation to abuse of other drugs by alveolar absorption and other effects of salbutamol. It is not known whether the dependency is due to the salbutamol itself or to the fluorinated hydrocarbons used as propellants.", "contents": "Dependency upon salbutamol inhalers. A case of dependency on salbutamol inhalers has been described. This has been discussed in relation to abuse of other drugs by alveolar absorption and other effects of salbutamol. It is not known whether the dependency is due to the salbutamol itself or to the fluorinated hydrocarbons used as propellants."}
{"id": "PMID:486241", "title": "Reaction of tetraethylammonium with the open and closed conformations of the acetylcholine receptor ionic channel complex.", "content": "The effect of tetraethylammonium (TEA) bromide on the neurally and iontophoretically evoked endplate current (EPC) of frog sartorius muscle was investigated using voltage-clamp and noise analysis techniques, and its binding to the acetylcholine (ACh) receptor ionic channel complex was determined on the electric organ of Torpedo ocellata. TEA (250-500 microM) produced an initial enhancement followed by a slow decline in the amplitude of the endplate potential and EPC, but caused only depression in the amplitude of the miniature endplate potential and current. In normal ringer's solution, the EPC current-voltage relationship was approximately linear, and the decay phase varied exponentially with membrane potential. Upon addition of 50-100 microM TEA, the current-voltage relationship became markedly nonlinear at hyperpolarized command potentials, and with 250-2000 microM TEA, there was an initial linear segment, an intermediate nonlinear segment, and a region of negative conductance. The onset of nonlinearity was dose-dependent, undergoing a 50 mV shift for a 10-fold increase in TEA concentration. The EPC decay phase was shortened by TEA at hyperpolarized but not depolarized potentials, and remained a single expotential function of time at all concentrations and membrane potentials examined. These actions of TEA were found to be independent of the sequence of polarizations, the length of the conditioning pulse, and the level of the initial holding potential. TEA shifted the power spectrum of ACh noise to higher frequencies and produced a significant depression of single channel conductance. The shortening in the mean channel lifetime agreed closely with the decrease in the EPC decay time constant. At the concentrations tested, TEA did not alter the EPC reversal potential, nor the resting membrane potential, and had little effect on the action potential duration. TEA inhibited the binding of both [3H] ACh (Ki = 200 microM) and [3H]perhydrohistrionicotoxin (Ki = 280 microM) to receptor-rich membranes from the electric organ of Torpedo ocellata, and inhibited the carbamylcholine-activated 22Na+ efflux from these microsacs. It is suggested that TEA reacts with the nicotinic ACh-receptor as well as its ion channel; the voltage-dependent actions are associated with blockade of the ion channel. The results are compatible with a kinetic model in which TEA first binds to the closed conformation of the receptor-ionicchannel complex to produce a voltage-depdndent depression of endplate conductance and sudsequently to its open conformation, giving rise to the shortening in the EPC decay and mean channel lifetime.", "contents": "Reaction of tetraethylammonium with the open and closed conformations of the acetylcholine receptor ionic channel complex. The effect of tetraethylammonium (TEA) bromide on the neurally and iontophoretically evoked endplate current (EPC) of frog sartorius muscle was investigated using voltage-clamp and noise analysis techniques, and its binding to the acetylcholine (ACh) receptor ionic channel complex was determined on the electric organ of Torpedo ocellata. TEA (250-500 microM) produced an initial enhancement followed by a slow decline in the amplitude of the endplate potential and EPC, but caused only depression in the amplitude of the miniature endplate potential and current. In normal ringer's solution, the EPC current-voltage relationship was approximately linear, and the decay phase varied exponentially with membrane potential. Upon addition of 50-100 microM TEA, the current-voltage relationship became markedly nonlinear at hyperpolarized command potentials, and with 250-2000 microM TEA, there was an initial linear segment, an intermediate nonlinear segment, and a region of negative conductance. The onset of nonlinearity was dose-dependent, undergoing a 50 mV shift for a 10-fold increase in TEA concentration. The EPC decay phase was shortened by TEA at hyperpolarized but not depolarized potentials, and remained a single expotential function of time at all concentrations and membrane potentials examined. These actions of TEA were found to be independent of the sequence of polarizations, the length of the conditioning pulse, and the level of the initial holding potential. TEA shifted the power spectrum of ACh noise to higher frequencies and produced a significant depression of single channel conductance. The shortening in the mean channel lifetime agreed closely with the decrease in the EPC decay time constant. At the concentrations tested, TEA did not alter the EPC reversal potential, nor the resting membrane potential, and had little effect on the action potential duration. TEA inhibited the binding of both [3H] ACh (Ki = 200 microM) and [3H]perhydrohistrionicotoxin (Ki = 280 microM) to receptor-rich membranes from the electric organ of Torpedo ocellata, and inhibited the carbamylcholine-activated 22Na+ efflux from these microsacs. It is suggested that TEA reacts with the nicotinic ACh-receptor as well as its ion channel; the voltage-dependent actions are associated with blockade of the ion channel. The results are compatible with a kinetic model in which TEA first binds to the closed conformation of the receptor-ionicchannel complex to produce a voltage-depdndent depression of endplate conductance and sudsequently to its open conformation, giving rise to the shortening in the EPC decay and mean channel lifetime."}
{"id": "PMID:486246", "title": "Gonorrhoea in women in Scotland. Evidence of cohorts having a higher or lower incidence than expected.", "content": "Incidences of genital gonorrhoea in women higher or lower than expected occurred in patients aged 15--19 years four years after similar findings in those aged 10--14 years. This pattern was followed five years later in those aged 20--24, 11 years later in those aged 25--34, and 20 years later in those aged 35--44 years. Thus, cohorts of women at greater or lesser risk of acquiring gonorrhoea appear to exist. Most cohorts with a high incidence could be identified when in the 10--14 age group. Especially high rates of infection are predicted in the 25--34 age group in the mid-1980s and in the 35--44 age group in the late 1980s. These findings will affect the timing of health education on sexually transmitted diseases in schools and will demand a high degree of awareness among general practitioners, gynaecologists, and those working in family planning and well-women clinics of the possibility of gonorrhoea occurring in women in these age groups.", "contents": "Gonorrhoea in women in Scotland. Evidence of cohorts having a higher or lower incidence than expected. Incidences of genital gonorrhoea in women higher or lower than expected occurred in patients aged 15--19 years four years after similar findings in those aged 10--14 years. This pattern was followed five years later in those aged 20--24, 11 years later in those aged 25--34, and 20 years later in those aged 35--44 years. Thus, cohorts of women at greater or lesser risk of acquiring gonorrhoea appear to exist. Most cohorts with a high incidence could be identified when in the 10--14 age group. Especially high rates of infection are predicted in the 25--34 age group in the mid-1980s and in the 35--44 age group in the late 1980s. These findings will affect the timing of health education on sexually transmitted diseases in schools and will demand a high degree of awareness among general practitioners, gynaecologists, and those working in family planning and well-women clinics of the possibility of gonorrhoea occurring in women in these age groups."}
{"id": "PMID:486247", "title": "How often are gonorrhoea and genital yeast infection sexually transmitted?", "content": "Although gonorrhoea is often regarded as the sexually transmitted disease against which others are measured, its infectivity is not clearly understood. Estimates of the infection rate have varied from 5--90%. In this study, 50 couples with gonorrhoea were matched with 50 couples with genital yeast infection. Gonorrhoea was diagnosed in both partners of 32 couples and genital yeast infection in both partners of 21 couples. These figures provide an indication of the sexual transmission of these conditions. The higher figure for gonorrhoea may be related to a greater urgency in tracing contacts.", "contents": "How often are gonorrhoea and genital yeast infection sexually transmitted? Although gonorrhoea is often regarded as the sexually transmitted disease against which others are measured, its infectivity is not clearly understood. Estimates of the infection rate have varied from 5--90%. In this study, 50 couples with gonorrhoea were matched with 50 couples with genital yeast infection. Gonorrhoea was diagnosed in both partners of 32 couples and genital yeast infection in both partners of 21 couples. These figures provide an indication of the sexual transmission of these conditions. The higher figure for gonorrhoea may be related to a greater urgency in tracing contacts."}
{"id": "PMID:486248", "title": "The need for a chlamydial culture service.", "content": "In a prospective study of unselected, female patients attending a sexually transmitted disease clinic one in eight patients would have been errouneously declared free of infection in the absence of a chlamydial culture service. Chlamydia trachomatis is now accepted as a causative organism of non-specific urethritis and post-gonococcal urethritis in men and non-specific genital infection in women. Thus, facilities for isolation of C. trachomatis should be an essential aid in the management of women attending STD clinics. Male patients would also benefit if such facilities were readily available.", "contents": "The need for a chlamydial culture service. In a prospective study of unselected, female patients attending a sexually transmitted disease clinic one in eight patients would have been errouneously declared free of infection in the absence of a chlamydial culture service. Chlamydia trachomatis is now accepted as a causative organism of non-specific urethritis and post-gonococcal urethritis in men and non-specific genital infection in women. Thus, facilities for isolation of C. trachomatis should be an essential aid in the management of women attending STD clinics. Male patients would also benefit if such facilities were readily available."}
{"id": "PMID:486251", "title": "[Statistical analysis of a neonatal service opened within a 2-year period].", "content": "We present a statistical study of unit 2 for newborns in Children's Hospital R. Guti\u00e9rrez, of the Buenos Aires municipality. An investigation was made which covered newborns who left the hospital from August 1976 to July 1978; during that period, 381 newborns finished their treatment. Summarizing the data assigned to death rate (22%) we could see a greater number during the first week; this tells us about the severe illness of the newborns that come into our service; we could also observe a significant mortality in newborns of high weight for their gestational age. The newborns with malformations have presented increased degree of illness and higher percentage of deaths (36%). From this review we had the idea of making a prospective study of our medical work, introducing, for a more correct evaluation, the degree of severeness when they come into the hospital and the classification of levels of high risk of the newborns.", "contents": "[Statistical analysis of a neonatal service opened within a 2-year period]. We present a statistical study of unit 2 for newborns in Children's Hospital R. Guti\u00e9rrez, of the Buenos Aires municipality. An investigation was made which covered newborns who left the hospital from August 1976 to July 1978; during that period, 381 newborns finished their treatment. Summarizing the data assigned to death rate (22%) we could see a greater number during the first week; this tells us about the severe illness of the newborns that come into our service; we could also observe a significant mortality in newborns of high weight for their gestational age. The newborns with malformations have presented increased degree of illness and higher percentage of deaths (36%). From this review we had the idea of making a prospective study of our medical work, introducing, for a more correct evaluation, the degree of severeness when they come into the hospital and the classification of levels of high risk of the newborns."}
{"id": "PMID:486252", "title": "[Counterimmunoelectrophoresis in the diagnosis of Bordetella pertussis].", "content": "The serum of 50 patients between 25 days to 15 years old who were admitted with the diagnosis of pertussis syndrome were investigated for precipitating antibodies by means of discontinuous counterimmunolectrophoresis (CIED). The presence of antibodies for Bordetella pertussis antigen was shown in 28 cases. All samples were taken within the first 24/48 hours of admission and at convalescence. The technique is easy to carry out, quick and of low cost. This test offers an adequate and fast means to differentiate a Bordetella pertussis syndrome form others produced by different viral etiologies. Besides, this serologic technique shows earlier results and of lower cost than classic techniques such as agglutination and complement fixation which are more difficult to apply.", "contents": "[Counterimmunoelectrophoresis in the diagnosis of Bordetella pertussis]. The serum of 50 patients between 25 days to 15 years old who were admitted with the diagnosis of pertussis syndrome were investigated for precipitating antibodies by means of discontinuous counterimmunolectrophoresis (CIED). The presence of antibodies for Bordetella pertussis antigen was shown in 28 cases. All samples were taken within the first 24/48 hours of admission and at convalescence. The technique is easy to carry out, quick and of low cost. This test offers an adequate and fast means to differentiate a Bordetella pertussis syndrome form others produced by different viral etiologies. Besides, this serologic technique shows earlier results and of lower cost than classic techniques such as agglutination and complement fixation which are more difficult to apply."}
{"id": "PMID:486253", "title": "[The triad syndrome].", "content": "The triad syndrome includes manifestations from urologic pathology to orthopedics, passing through the gastrointestinal to the respiratory pathology. Treatment of these syndromes calls for an intensive management, carried out by a multidisciplinarian pediatric team, to whom the surgical technique is as important as the genetic counseling; this is the only way to offer these children, who have been precociously damaged, a happy infancy. On the other hand, it is the purpose of this work to make an early discovery of congenital malformations, detecting their minimal expressions, which is the only manner to reach an early diagnosis so as to indicate an adequate therapy at the opportune moment. All this added to the fact that in 1973-1975 we gathered a few cases of triple syndrome, plus our limited bibliography (Rahman) resulted in the necessity to bring up-to-date the topic. At present, 5 years later, the satisfactory course in these patients has allowed us to indicate guidelines in the diagnosis and treatment.", "contents": "[The triad syndrome]. The triad syndrome includes manifestations from urologic pathology to orthopedics, passing through the gastrointestinal to the respiratory pathology. Treatment of these syndromes calls for an intensive management, carried out by a multidisciplinarian pediatric team, to whom the surgical technique is as important as the genetic counseling; this is the only way to offer these children, who have been precociously damaged, a happy infancy. On the other hand, it is the purpose of this work to make an early discovery of congenital malformations, detecting their minimal expressions, which is the only manner to reach an early diagnosis so as to indicate an adequate therapy at the opportune moment. All this added to the fact that in 1973-1975 we gathered a few cases of triple syndrome, plus our limited bibliography (Rahman) resulted in the necessity to bring up-to-date the topic. At present, 5 years later, the satisfactory course in these patients has allowed us to indicate guidelines in the diagnosis and treatment."}
{"id": "PMID:486254", "title": "[Noonan syndrome: differential diagnosis with Turner's syndrome].", "content": "Clinical and laboratory evidences assure an unequivocal identity to the syndrome described by Noonan. We believed that the terminology used by many authors has contributed to maintain confusion with Turner's syndrome from which it is clearly differenciated. The signology of both syndromes was confrontated in order to delineate the syndrome. Emphasis was made to point out the signs which are proper to each syndrome and the signs which are common to both of them stressing those that, occur with equal or significant difference. Two new signs are described in Noonan syndrome: alopecia of the hund portions of the eyebrows and keratosis rubra pilaris (Ulerythema ophriogenes).", "contents": "[Noonan syndrome: differential diagnosis with Turner's syndrome]. Clinical and laboratory evidences assure an unequivocal identity to the syndrome described by Noonan. We believed that the terminology used by many authors has contributed to maintain confusion with Turner's syndrome from which it is clearly differenciated. The signology of both syndromes was confrontated in order to delineate the syndrome. Emphasis was made to point out the signs which are proper to each syndrome and the signs which are common to both of them stressing those that, occur with equal or significant difference. Two new signs are described in Noonan syndrome: alopecia of the hund portions of the eyebrows and keratosis rubra pilaris (Ulerythema ophriogenes)."}
{"id": "PMID:486255", "title": "[Problems in the management of patients with inoperable cardiac anomalies].", "content": "A general review of the main problems found in the management of inoperable heart anomalies is made by the author. He determines the reasons why a heart disease cannot be corrected by surgery, the complications found in such cases, the cautiousness that must be taken and the psychological aspect of these problems in relationship with the family and the patient.", "contents": "[Problems in the management of patients with inoperable cardiac anomalies]. A general review of the main problems found in the management of inoperable heart anomalies is made by the author. He determines the reasons why a heart disease cannot be corrected by surgery, the complications found in such cases, the cautiousness that must be taken and the psychological aspect of these problems in relationship with the family and the patient."}
{"id": "PMID:486256", "title": "[Nutrition surveys in children attending kindergartens in the city of Cordoba].", "content": "A nutritional inquest was carried out in 1857 children attending kindergartens in C\u00f3rdoba, Argentina, in 1974. Determinations were made for the socioeconomical level each child belonged (NES) and clinical and anthropometric examinations. Nutritional inquests were carried out in 414 of these children, psychological examinations in 51, and biochemical inquests in 61 children.", "contents": "[Nutrition surveys in children attending kindergartens in the city of Cordoba]. A nutritional inquest was carried out in 1857 children attending kindergartens in C\u00f3rdoba, Argentina, in 1974. Determinations were made for the socioeconomical level each child belonged (NES) and clinical and anthropometric examinations. Nutritional inquests were carried out in 414 of these children, psychological examinations in 51, and biochemical inquests in 61 children."}
{"id": "PMID:486257", "title": "[Recovery of breast feeding practice].", "content": "The failure to recover the period of motherly suckling originated the creation of a community institution: Mother's Help Group NuNu, which is looking for a conherence promotion. In this way, not only do women make the community and individual diffusion of the human lactation but a pediatric doctor also contributes to bring up children, with human resource, laying aside technical excess.", "contents": "[Recovery of breast feeding practice]. The failure to recover the period of motherly suckling originated the creation of a community institution: Mother's Help Group NuNu, which is looking for a conherence promotion. In this way, not only do women make the community and individual diffusion of the human lactation but a pediatric doctor also contributes to bring up children, with human resource, laying aside technical excess."}
{"id": "PMID:486259", "title": "[Incidence of social factors in the care of children in the urban and semiurban area of Salta].", "content": "The authors analyse the social histories of 1000 children taking into account their place of origin, state of nutrition, age of the child and of his parents; their social condition, number of offsprings, wages, degree of instruction and profession of the parents, ledging: quality, running water, sanitary and electric services: type of feeding, conditions for preservation of food. All this information is important to the physician because it implies that the patient treated in the hospital should not return to an adverse environment where he will find the same factors that will lead him again to disease.", "contents": "[Incidence of social factors in the care of children in the urban and semiurban area of Salta]. The authors analyse the social histories of 1000 children taking into account their place of origin, state of nutrition, age of the child and of his parents; their social condition, number of offsprings, wages, degree of instruction and profession of the parents, ledging: quality, running water, sanitary and electric services: type of feeding, conditions for preservation of food. All this information is important to the physician because it implies that the patient treated in the hospital should not return to an adverse environment where he will find the same factors that will lead him again to disease."}
{"id": "PMID:486261", "title": "[Acute benign ataxia in childhood].", "content": "The patogenesis and etiology of acute ataxia in childhood is not well known. It may occur without previous symptoms or may be the expression of specific infectious diseases. Forty patients hospitalized at the Hospital de Ni\u00f1os de Buenos Aires en 1972-1978, were studied. The neurological manifestations showed an acute onset, being ataxia the main sign, associate to tremor, nystagmus, dysartria, oculo-motor paresia, muscular weakness, and hyporeflexia. Most of the patients (82%) became cured within the first four weeks. It is advisable to establish a follow-up with periodic controls, mainly in those patients in whom an association with previous infectious diseases did not exist to be able to detect an association with degenerative or desmyelinizing diseases.", "contents": "[Acute benign ataxia in childhood]. The patogenesis and etiology of acute ataxia in childhood is not well known. It may occur without previous symptoms or may be the expression of specific infectious diseases. Forty patients hospitalized at the Hospital de Ni\u00f1os de Buenos Aires en 1972-1978, were studied. The neurological manifestations showed an acute onset, being ataxia the main sign, associate to tremor, nystagmus, dysartria, oculo-motor paresia, muscular weakness, and hyporeflexia. Most of the patients (82%) became cured within the first four weeks. It is advisable to establish a follow-up with periodic controls, mainly in those patients in whom an association with previous infectious diseases did not exist to be able to detect an association with degenerative or desmyelinizing diseases."}
{"id": "PMID:486262", "title": "[Mediastinal emphysema in children].", "content": "A review of 25 infants developing pneumediastinum and subcutaneous emphysema was done. These observations suggested that increasing airways presure creates excessive alveolar distention. Pulmonary interstitial emphysema results from movement of air out of alveoli into interstitial areas. From this point air could dissect along the perivascular or interstitial spaces to the mediastinum.", "contents": "[Mediastinal emphysema in children]. A review of 25 infants developing pneumediastinum and subcutaneous emphysema was done. These observations suggested that increasing airways presure creates excessive alveolar distention. Pulmonary interstitial emphysema results from movement of air out of alveoli into interstitial areas. From this point air could dissect along the perivascular or interstitial spaces to the mediastinum."}
{"id": "PMID:486264", "title": "[Infantile and juvenile violent death].", "content": "Infantile and youth violent death has been analyzed with a new approach using an informative source different from traditional ones by means of a dynamic statement with a retrospective study of the process. We call attention to the great number of violent deaths that affect the newborn, the infant and the adolescent. We insist on the way that violent death occurs through its three mechanism: accidental, suicide or homicide. We set the difference among natural, sudden, doubtful and violent death. We observe the increasing number of infantile and youth necropsies made at the judicial morgue under the name: violent death, in the studied periods. We stress the preponderance of males in all the causes of infantile and youth violent death by means of programs related to the three ways in which violent death occurs: accident, homicide and suicide.", "contents": "[Infantile and juvenile violent death]. Infantile and youth violent death has been analyzed with a new approach using an informative source different from traditional ones by means of a dynamic statement with a retrospective study of the process. We call attention to the great number of violent deaths that affect the newborn, the infant and the adolescent. We insist on the way that violent death occurs through its three mechanism: accidental, suicide or homicide. We set the difference among natural, sudden, doubtful and violent death. We observe the increasing number of infantile and youth necropsies made at the judicial morgue under the name: violent death, in the studied periods. We stress the preponderance of males in all the causes of infantile and youth violent death by means of programs related to the three ways in which violent death occurs: accident, homicide and suicide."}
{"id": "PMID:486258", "title": "[Diabetes mellitus in childhood and adolescence. Clinical types].", "content": "It is today's general medical opinion that children's diabetes mellitus was uncommon in the past. It was generally admitted at that time the initail stages were so sudden as to make difficut its early diagnosis. It's increased incidence is at present an alarming truth; however, a parallel increase of diabetic coma or of mulminant types has rather dropped. Diabetes may be diagnosed by just considering the main symptoms at the onset which are polydipsia, polyuria and weight loss. If an early diagnosis is not made, acidosis (abdominal pain, nausea, vomiting) may appear within a few days or weeks followed by coma (Kussamul's acidotic respiration and dehydration). Coma may be avoided by an early diagnosis and a life may be saved. It must be stressed that an important percentage of children and adolescents show a slow and gradual evolution (week or months) of their diabetes: gradual weight loss, sometimes with noticeable polyphagia, occasional enuresis, but without other associated symptoms. Asymptomatic, intermittent glucosurias are also frequent; they vary in magnitude an almost always they appear without ketonuria and with fasting normal glycemia. According to our experience they may precede in weeks or months the clinical manifestations of the disease. Postprandial glycemia is a sure diagnostic resource; it is of greater trustworthines than fasting glycemia; therefore we advise it as a routine diagnostic procedure which we recommend widely. In uncertain situations, the oral glucose tolerance test is advisable.", "contents": "[Diabetes mellitus in childhood and adolescence. Clinical types]. It is today's general medical opinion that children's diabetes mellitus was uncommon in the past. It was generally admitted at that time the initail stages were so sudden as to make difficut its early diagnosis. It's increased incidence is at present an alarming truth; however, a parallel increase of diabetic coma or of mulminant types has rather dropped. Diabetes may be diagnosed by just considering the main symptoms at the onset which are polydipsia, polyuria and weight loss. If an early diagnosis is not made, acidosis (abdominal pain, nausea, vomiting) may appear within a few days or weeks followed by coma (Kussamul's acidotic respiration and dehydration). Coma may be avoided by an early diagnosis and a life may be saved. It must be stressed that an important percentage of children and adolescents show a slow and gradual evolution (week or months) of their diabetes: gradual weight loss, sometimes with noticeable polyphagia, occasional enuresis, but without other associated symptoms. Asymptomatic, intermittent glucosurias are also frequent; they vary in magnitude an almost always they appear without ketonuria and with fasting normal glycemia. According to our experience they may precede in weeks or months the clinical manifestations of the disease. Postprandial glycemia is a sure diagnostic resource; it is of greater trustworthines than fasting glycemia; therefore we advise it as a routine diagnostic procedure which we recommend widely. In uncertain situations, the oral glucose tolerance test is advisable."}
{"id": "PMID:486271", "title": "Low voltage electrical activity preceding right atrial depolarisation in man.", "content": "Electrical recordings were made in the high right atrium in 28 patients undergoing cardiac catheterisation and in 3 healthy volunteers. After filtering and amplification by 3 to 10 million times, the signals were passed through a signal averaging process in a digital computer. Of the 28 subjects who had technically satisfactory recordings, 23 showed low voltage electrical activity preceding the conventionally-recorded atrial depolarisation. The low voltage activity started 50 to 200 ms before the atrial deflection and was variable in shape. These early signals may be the result of activity in the region of the sinus node.", "contents": "Low voltage electrical activity preceding right atrial depolarisation in man. Electrical recordings were made in the high right atrium in 28 patients undergoing cardiac catheterisation and in 3 healthy volunteers. After filtering and amplification by 3 to 10 million times, the signals were passed through a signal averaging process in a digital computer. Of the 28 subjects who had technically satisfactory recordings, 23 showed low voltage electrical activity preceding the conventionally-recorded atrial depolarisation. The low voltage activity started 50 to 200 ms before the atrial deflection and was variable in shape. These early signals may be the result of activity in the region of the sinus node."}
{"id": "PMID:486263", "title": "[Various aspects of the influence of infection on growth and development].", "content": "It is pointed out how infections can delay growth and development through an aggressive and long-standing action, through direct action of an infecting agent (virus, bacteria, parasites). or through sequelae in main organs (brain, kidney, growth cartilage, muscular system, etc.). how infections impair nutrients' absortion, metabolism and excretion. The role of the leukocytes is evidenced in the liberation of two factors: MEL and pyrogen factor. Metabolic responses are classified in an anabolic anda catabolic phase. The second mechanism that interfers on growth and development is a specific action of various agents, particularly viruses on different tissues. Intrauterine infection is pointed out as determinant of growth disorders with increased IgM levels in cord blood; confirmed fetal infection on the 6th up to 8th week of gestation interferes with organogenesis. The action of CMV and rubella virus is mentioned as example. The action of viral infections on growth during the first three months of gestation, is mentioned. TBK and typhoid fever are mentioned as causative agents of corporal and urinary nitrogen loss. Negative action of infection on growth and development, particularly in malnourished patients, is discussed. Diarrhea, under certain condition, is pointed out as determinant of the kwashiorkor syndrome. The role of various parasite diseases on growth is mentioned with range according to the action of each agent on nutrients' absortion. A third mechanism which causes fail to thrive through sequelae lesions is considered. This disorders are ruled by the age of the patient with the onset of disease by the causative agent, by the inflammatory process involved, by the early diagnosis and appropriate treatment. The need of enhancing the actions to prevent communicable diseases and to diminish malnutrition rates is pointed out and the significance of taking into account calorie and protein intake in patients with severe and/or long-standing infectious disease.", "contents": "[Various aspects of the influence of infection on growth and development]. It is pointed out how infections can delay growth and development through an aggressive and long-standing action, through direct action of an infecting agent (virus, bacteria, parasites). or through sequelae in main organs (brain, kidney, growth cartilage, muscular system, etc.). how infections impair nutrients' absortion, metabolism and excretion. The role of the leukocytes is evidenced in the liberation of two factors: MEL and pyrogen factor. Metabolic responses are classified in an anabolic anda catabolic phase. The second mechanism that interfers on growth and development is a specific action of various agents, particularly viruses on different tissues. Intrauterine infection is pointed out as determinant of growth disorders with increased IgM levels in cord blood; confirmed fetal infection on the 6th up to 8th week of gestation interferes with organogenesis. The action of CMV and rubella virus is mentioned as example. The action of viral infections on growth during the first three months of gestation, is mentioned. TBK and typhoid fever are mentioned as causative agents of corporal and urinary nitrogen loss. Negative action of infection on growth and development, particularly in malnourished patients, is discussed. Diarrhea, under certain condition, is pointed out as determinant of the kwashiorkor syndrome. The role of various parasite diseases on growth is mentioned with range according to the action of each agent on nutrients' absortion. A third mechanism which causes fail to thrive through sequelae lesions is considered. This disorders are ruled by the age of the patient with the onset of disease by the causative agent, by the inflammatory process involved, by the early diagnosis and appropriate treatment. The need of enhancing the actions to prevent communicable diseases and to diminish malnutrition rates is pointed out and the significance of taking into account calorie and protein intake in patients with severe and/or long-standing infectious disease."}
{"id": "PMID:486272", "title": "Effect of nifedipine on atrioventricular conduction as compared with verapamil. Intracardiac electrophysiological study.", "content": "Intravenous nifedipine, a powerful calcium antagonist, had no obvious effect on atrioventricular conduction when administered to 11 patients during routine intracardiac electrophysiological studies. Verapamil on the other hand showed potent antiarrhythmic properties, depressing atrioventricular nodal conduction. Nifedipine thus appears safe in patients with angina pectoris who have disorders of atrioventricular nodal conduction, and in those receiving beta-adrenergic blocking drugs. There appear to be differential effects on the slow inward channels of cardiac cells with different 'calcium antagonists'.", "contents": "Effect of nifedipine on atrioventricular conduction as compared with verapamil. Intracardiac electrophysiological study. Intravenous nifedipine, a powerful calcium antagonist, had no obvious effect on atrioventricular conduction when administered to 11 patients during routine intracardiac electrophysiological studies. Verapamil on the other hand showed potent antiarrhythmic properties, depressing atrioventricular nodal conduction. Nifedipine thus appears safe in patients with angina pectoris who have disorders of atrioventricular nodal conduction, and in those receiving beta-adrenergic blocking drugs. There appear to be differential effects on the slow inward channels of cardiac cells with different 'calcium antagonists'."}
{"id": "PMID:486274", "title": "Recurrence of myocardial infarction in an exercising population.", "content": "The Ontario Multi-Centre Exercise-Heart trial is making a prospective study of 751 male subjects following well-documented episodes of myocardial infarction. Comparison is here made between the 50 participants who sustained a recurrence of their myocardial infarction, and the 701 participants who did not. Reinfarction was a little more likely with a history of multiple previous infarctions, but was unrelated to such indicators of infarct severity as symptoms, electrocardiographic abnormalities, enzyme changes, cardiac arrest, arrhythmia, or minimum systolic blood pressure. Features noted on admission to the trial, which may have an adverse effect, include smoking history and related symptoms, residual disability, shortness of breath, and angina of effort. The main physiological warning sign was a low and decreasing cardiac output at a submaximal work load, with a compensatory widening of arteriovenous oxygen difference. With the possible exception of exercise non-compliance, none of the adverse findings is sufficiently consistent to be of value when advising individual patients.", "contents": "Recurrence of myocardial infarction in an exercising population. The Ontario Multi-Centre Exercise-Heart trial is making a prospective study of 751 male subjects following well-documented episodes of myocardial infarction. Comparison is here made between the 50 participants who sustained a recurrence of their myocardial infarction, and the 701 participants who did not. Reinfarction was a little more likely with a history of multiple previous infarctions, but was unrelated to such indicators of infarct severity as symptoms, electrocardiographic abnormalities, enzyme changes, cardiac arrest, arrhythmia, or minimum systolic blood pressure. Features noted on admission to the trial, which may have an adverse effect, include smoking history and related symptoms, residual disability, shortness of breath, and angina of effort. The main physiological warning sign was a low and decreasing cardiac output at a submaximal work load, with a compensatory widening of arteriovenous oxygen difference. With the possible exception of exercise non-compliance, none of the adverse findings is sufficiently consistent to be of value when advising individual patients."}
{"id": "PMID:486275", "title": "Assessment of pulmonary valve echogram in normal subjects and in patients with pulmonary arterial hypertension.", "content": "To assess the validity of ultrasound criteria for pulmonary arterial hypertension, we studied pulmonary valve motion in 28 patients and 20 normal subjects. In the latter group, we categorised normal movement of the posterior leaflet of the pulmonary valve in a fashion not previously described. Of the 28 patients, 19 had pulmonary arterial hypertension (pulmonary artery mean pressure more than 20 mmHg, range 22 to 72). Negative, flat, and positive e to f slopes occurred equally in normal subjects and patients. Maximal a wave excursion was less than 2 mm in 9 of 13 patients with pulmonary hypertension who had sinus rhythm, but was more than 2 mm in all normals and in the 9 patients with normal pulmonary artery pressure (69% sensitivity and 100% specificity). The b to c slope was more than 450 mm/s in 6 of 18 patients with pulmonary arterial hypertension and was less than 450 mm/s in all others (33% sensitivity and 100% specificity). The normalised right ventricular pre-ejection period was more than 0.095 (range 0.10 to 0.16) in 13 of the 19 patients with pulmonary arterial hypertension and less than 0.095 in all others (68% sensitivity and 100% specificity). A midsystolic notch occurred in patients with pulmonary arterial hypertension and in no normal subjects or patients with normal pulmonary arterial pressure. Of the 19 patients with pulmonary arterial hypertension, 18 were identified by one or more ultrasound criteria. Of the 5 patients who met only 1 criterion (increased normalised right ventricular pre-ejection period), 4 had atrial fibrillation. We conclude that measurement of the pulmonary valve e to f slope is useless for identifying pulmonary arterial hypertension. However, changes in normalised right ventricular pre-ejection, maximal a wave excursion, b to c slope, and the presence of a midsystolic notch, while insensitive, are highly specific for pulmonary atrial hypertension.", "contents": "Assessment of pulmonary valve echogram in normal subjects and in patients with pulmonary arterial hypertension. To assess the validity of ultrasound criteria for pulmonary arterial hypertension, we studied pulmonary valve motion in 28 patients and 20 normal subjects. In the latter group, we categorised normal movement of the posterior leaflet of the pulmonary valve in a fashion not previously described. Of the 28 patients, 19 had pulmonary arterial hypertension (pulmonary artery mean pressure more than 20 mmHg, range 22 to 72). Negative, flat, and positive e to f slopes occurred equally in normal subjects and patients. Maximal a wave excursion was less than 2 mm in 9 of 13 patients with pulmonary hypertension who had sinus rhythm, but was more than 2 mm in all normals and in the 9 patients with normal pulmonary artery pressure (69% sensitivity and 100% specificity). The b to c slope was more than 450 mm/s in 6 of 18 patients with pulmonary arterial hypertension and was less than 450 mm/s in all others (33% sensitivity and 100% specificity). The normalised right ventricular pre-ejection period was more than 0.095 (range 0.10 to 0.16) in 13 of the 19 patients with pulmonary arterial hypertension and less than 0.095 in all others (68% sensitivity and 100% specificity). A midsystolic notch occurred in patients with pulmonary arterial hypertension and in no normal subjects or patients with normal pulmonary arterial pressure. Of the 19 patients with pulmonary arterial hypertension, 18 were identified by one or more ultrasound criteria. Of the 5 patients who met only 1 criterion (increased normalised right ventricular pre-ejection period), 4 had atrial fibrillation. We conclude that measurement of the pulmonary valve e to f slope is useless for identifying pulmonary arterial hypertension. However, changes in normalised right ventricular pre-ejection, maximal a wave excursion, b to c slope, and the presence of a midsystolic notch, while insensitive, are highly specific for pulmonary atrial hypertension."}
{"id": "PMID:486279", "title": "Heart rhythm during permanent cardiac pacing.", "content": "Heart rhythm was analysed with regard to spontaneous or pacemaker-induced heart activity, in a consecutive series of 282 patients paced for at least 1 year. The mean duration of pacing was 59 (13 to 180) months. The mean age of the patients was 76 (39 to 93) years. Spontaneous heart activity at all routine examinations was found in 33 (12%) of the patients. Pacemaker-induced rhythm only was recorded in 42 per cent of the patients whereas the remaining 46 per cent had varying electrocardiographic patterns. Of the patients with spontaneous rhythm at each visit, 10 had had complete heart block before pacing. Regular sinus activity was recorded at every routine examination in 74 per cent of the patients paced for reasons other than the sick sinus syndrome. This indicated that a substantial number of paced patients might be candidates for atrial triggered pacing. Patients treated with digitalis more often had asystole at the time of replacement of the pacemaker (32%) than those not so treated (19). This suggests an increased risk of sudden death in paced patients on digitalis if the pacemaker fails.", "contents": "Heart rhythm during permanent cardiac pacing. Heart rhythm was analysed with regard to spontaneous or pacemaker-induced heart activity, in a consecutive series of 282 patients paced for at least 1 year. The mean duration of pacing was 59 (13 to 180) months. The mean age of the patients was 76 (39 to 93) years. Spontaneous heart activity at all routine examinations was found in 33 (12%) of the patients. Pacemaker-induced rhythm only was recorded in 42 per cent of the patients whereas the remaining 46 per cent had varying electrocardiographic patterns. Of the patients with spontaneous rhythm at each visit, 10 had had complete heart block before pacing. Regular sinus activity was recorded at every routine examination in 74 per cent of the patients paced for reasons other than the sick sinus syndrome. This indicated that a substantial number of paced patients might be candidates for atrial triggered pacing. Patients treated with digitalis more often had asystole at the time of replacement of the pacemaker (32%) than those not so treated (19). This suggests an increased risk of sudden death in paced patients on digitalis if the pacemaker fails."}
{"id": "PMID:486278", "title": "Use of digitised left ventricular echocardiograms in assessment of mitral stenosis.", "content": "In order to establish a new echocardiographic index of the severity of mitral stenosis, left ventricular echocardiograms were digitised using manual tracing and a computer. The instantaneous left ventricular dimension was measured in 15 patients with mitral stenosis. The peak rate of change of left ventricular dimension (peak dD/dt) and the normalised peak rate (peak dD/dt/D) during early diastole were significantly lower in mitral stenosis than in normal subjects. Also, the time between the second heart sound and peak dD/dt was significantly shorter in patients with mitral stenosis than in normal subjects. Correlation between the mitral valve area calculated from the Gorlin formula and peak dD/dt/D was good, while valve area was poorly correlated with the EF slope of the anterior mitral leaflet echo. It is suggested that by continuous measurement of left ventricular dimension and of its first derivative, the reduced atrioventricular blood flow in mitral stenosis can be shown, and that peak dD/dt/D during early diastole is a possible index of its severity.", "contents": "Use of digitised left ventricular echocardiograms in assessment of mitral stenosis. In order to establish a new echocardiographic index of the severity of mitral stenosis, left ventricular echocardiograms were digitised using manual tracing and a computer. The instantaneous left ventricular dimension was measured in 15 patients with mitral stenosis. The peak rate of change of left ventricular dimension (peak dD/dt) and the normalised peak rate (peak dD/dt/D) during early diastole were significantly lower in mitral stenosis than in normal subjects. Also, the time between the second heart sound and peak dD/dt was significantly shorter in patients with mitral stenosis than in normal subjects. Correlation between the mitral valve area calculated from the Gorlin formula and peak dD/dt/D was good, while valve area was poorly correlated with the EF slope of the anterior mitral leaflet echo. It is suggested that by continuous measurement of left ventricular dimension and of its first derivative, the reduced atrioventricular blood flow in mitral stenosis can be shown, and that peak dD/dt/D during early diastole is a possible index of its severity."}
{"id": "PMID:486281", "title": "Patterns of atrial activation during right ventricular pacing in patients with concealed left-sided Kent pathways.", "content": "A 'concealed' accessory pathway was suspected in 12 patients because of eccentric left atrial activation during tachycardia. Retrograde conduction during ventricular pacing may occur over the atrioventricular node, the accessory pathway, or both. There were 4 patterns of ventriculoatrial conduction in response to ventricular extrastimuli (V2) at various coupling intervals: (1) exclusive accessory pathway conduction throughout the cardiac cycle in 2 patients; (2) exclusive accessory pathway conduction at long coupling intervals and exclusive atrioventricular node conduction at short coupling intervals in 2 patients; (3) variably fused accessory pathway/atrioventricular node conduction at long coupling intervals but exclusive accessory pathway conduction at short coupling intervals in 4 patients; (4) fused accessory pathway/atrioventricular node conduction at long coupling intervals but exclusive atrioventricular node conduction at short coupling intervals in 4 patients. With increased prematurity of V2 the ventricle to right atrial interval prolonged conspicuously in 11 of 12 patients whereas the ventricle to left atrial interval remained constant until the refractory period of the accessory pathway in all but 2 instances where intraventricular delay occurred. This study emphasises the importance of left atrial recordings in these patients.", "contents": "Patterns of atrial activation during right ventricular pacing in patients with concealed left-sided Kent pathways. A 'concealed' accessory pathway was suspected in 12 patients because of eccentric left atrial activation during tachycardia. Retrograde conduction during ventricular pacing may occur over the atrioventricular node, the accessory pathway, or both. There were 4 patterns of ventriculoatrial conduction in response to ventricular extrastimuli (V2) at various coupling intervals: (1) exclusive accessory pathway conduction throughout the cardiac cycle in 2 patients; (2) exclusive accessory pathway conduction at long coupling intervals and exclusive atrioventricular node conduction at short coupling intervals in 2 patients; (3) variably fused accessory pathway/atrioventricular node conduction at long coupling intervals but exclusive accessory pathway conduction at short coupling intervals in 4 patients; (4) fused accessory pathway/atrioventricular node conduction at long coupling intervals but exclusive atrioventricular node conduction at short coupling intervals in 4 patients. With increased prematurity of V2 the ventricle to right atrial interval prolonged conspicuously in 11 of 12 patients whereas the ventricle to left atrial interval remained constant until the refractory period of the accessory pathway in all but 2 instances where intraventricular delay occurred. This study emphasises the importance of left atrial recordings in these patients."}
{"id": "PMID:486282", "title": "Assessment of left ventricular performance and myocardial viability using quantitative radioisotope techniques.", "content": "The diagnostic value of a combined radionuclide technique was compared with conventional angiocardiographic techniques in 60 patients with coronary artery disease. Quantitative 201Tl myocardial imaging combined with radionuclide angiocardiography using 99mTc-HSA provided a safe and accurate method for the assessment of left ventricular performance. The defects on the 201Tl images correlated with the severity of asynergy seen on the contrast ventriculogram. Static imaging alone distinguished hypokinetic from akinetic or dyskinetic areas. However, using both tracer techniques, akinesis could bedistinguished from dyskinesis. In patients with disturbed left ventricular function, cardiac transit times correlated with haemodynamic changes, and left ventricular ejection fraction was the most sensitive index. Thus, this combined radionuclide approach provides data for the evaluation of overall and regional wall function. A major advantage of this non-invasive auantitative technique is its applicability to the critically ill patient at the bedside.", "contents": "Assessment of left ventricular performance and myocardial viability using quantitative radioisotope techniques. The diagnostic value of a combined radionuclide technique was compared with conventional angiocardiographic techniques in 60 patients with coronary artery disease. Quantitative 201Tl myocardial imaging combined with radionuclide angiocardiography using 99mTc-HSA provided a safe and accurate method for the assessment of left ventricular performance. The defects on the 201Tl images correlated with the severity of asynergy seen on the contrast ventriculogram. Static imaging alone distinguished hypokinetic from akinetic or dyskinetic areas. However, using both tracer techniques, akinesis could bedistinguished from dyskinesis. In patients with disturbed left ventricular function, cardiac transit times correlated with haemodynamic changes, and left ventricular ejection fraction was the most sensitive index. Thus, this combined radionuclide approach provides data for the evaluation of overall and regional wall function. A major advantage of this non-invasive auantitative technique is its applicability to the critically ill patient at the bedside."}
{"id": "PMID:486283", "title": "Plasma adrenaline and noradrenaline concentrations and dopamine-beta-hydroxylase activity in myocardial infarction with and without cardiogenic shock.", "content": "In patients with myocardial infarction, with or without cardiogenic shock, plasma noradrenaline and adrenaline concentrations are increased. However, plasma noradrenaline concentrations are considerably higher in patients with cardiogenic shock when compared with those with uncomplicated myocardial infarction. Plasma noradrenaline and adrenaline concentrations showed a sustained increase until death in patients with cardiogenic shock whereas those concentrations were back to normal levels by the end of the third day in patients with uncomplicated myocardial infarction. Plasma dopamine-beta-hydroxylase activities in both the groups were within normal range and did not show any significant variation throughout the period of study.", "contents": "Plasma adrenaline and noradrenaline concentrations and dopamine-beta-hydroxylase activity in myocardial infarction with and without cardiogenic shock. In patients with myocardial infarction, with or without cardiogenic shock, plasma noradrenaline and adrenaline concentrations are increased. However, plasma noradrenaline concentrations are considerably higher in patients with cardiogenic shock when compared with those with uncomplicated myocardial infarction. Plasma noradrenaline and adrenaline concentrations showed a sustained increase until death in patients with cardiogenic shock whereas those concentrations were back to normal levels by the end of the third day in patients with uncomplicated myocardial infarction. Plasma dopamine-beta-hydroxylase activities in both the groups were within normal range and did not show any significant variation throughout the period of study."}
{"id": "PMID:486284", "title": "Myxoma of the mitral valve.", "content": "A case of myxoma of the mitral valve is reported. The clinical features were indistinguishable from left atrial myxoma with prolapse through the mitral valve. Because of the known tendency for these tumours to recur it was treated by wide excision which necessitated replacement of the mitral valve.", "contents": "Myxoma of the mitral valve. A case of myxoma of the mitral valve is reported. The clinical features were indistinguishable from left atrial myxoma with prolapse through the mitral valve. Because of the known tendency for these tumours to recur it was treated by wide excision which necessitated replacement of the mitral valve."}
{"id": "PMID:486285", "title": "Constrictive endocarditis. Report of a case with successful surgery.", "content": "An 18-year old white youth presented with severe right heart failure and was found to have an obliterated and funnel-shaped right ventricle, massive tricuspid regurgitation, and mitral regurgitation. The haemodynamic findings were those of cardiomyopathy with obliteration on the right side. He underwent surgery consisting of decortication and peeling off of a thickened right ventricular endocardium, tricuspid valve replacement, and mitral valve repair. After surgery there was pronounced haemodynamic as well as clinical improvement. The pathological picture was that of constrictive endocarditis. We recommend this method of treatment for patients with obliterate cardiomyopathy on either side of the heart.", "contents": "Constrictive endocarditis. Report of a case with successful surgery. An 18-year old white youth presented with severe right heart failure and was found to have an obliterated and funnel-shaped right ventricle, massive tricuspid regurgitation, and mitral regurgitation. The haemodynamic findings were those of cardiomyopathy with obliteration on the right side. He underwent surgery consisting of decortication and peeling off of a thickened right ventricular endocardium, tricuspid valve replacement, and mitral valve repair. After surgery there was pronounced haemodynamic as well as clinical improvement. The pathological picture was that of constrictive endocarditis. We recommend this method of treatment for patients with obliterate cardiomyopathy on either side of the heart."}
{"id": "PMID:486286", "title": "Lone aortic regurgitation, sacroiliitis, and HLA B27. Case history and frequency of association.", "content": "An account of aortic regurgitation complicating ankylosing spondylitis is given. Twenty patients with lone aortic regurgitation and without overt spondylitis were examined clinically and radiologically and tissue typed. No evidence of sacroiliitis could be found in any patient. HLA B27 was absent from this group, and no significant disturbance in antigen frequency was noted.", "contents": "Lone aortic regurgitation, sacroiliitis, and HLA B27. Case history and frequency of association. An account of aortic regurgitation complicating ankylosing spondylitis is given. Twenty patients with lone aortic regurgitation and without overt spondylitis were examined clinically and radiologically and tissue typed. No evidence of sacroiliitis could be found in any patient. HLA B27 was absent from this group, and no significant disturbance in antigen frequency was noted."}
{"id": "PMID:486288", "title": "The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes: relevance to digoxin therapy.", "content": "1 The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes are described. 2 Only one class of binding site can be demonstrated. Binding is time- and temperature-dependent, saturable and slowly reversible; it is inhibited by other cardiac glycosides and by potassium. 3 Pre-incubation with unlabelled digoxin reduces the subsequent binding of 12-alpha-[3H]-digoxin in stoichiometric fashion. 4 The possible application of the measurement of the binding of 12-alpha-[3H]-digoxin to the study of biochemical pharmacological events occurring during digoxin therapy is discussed.", "contents": "The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes: relevance to digoxin therapy. 1 The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes are described. 2 Only one class of binding site can be demonstrated. Binding is time- and temperature-dependent, saturable and slowly reversible; it is inhibited by other cardiac glycosides and by potassium. 3 Pre-incubation with unlabelled digoxin reduces the subsequent binding of 12-alpha-[3H]-digoxin in stoichiometric fashion. 4 The possible application of the measurement of the binding of 12-alpha-[3H]-digoxin to the study of biochemical pharmacological events occurring during digoxin therapy is discussed."}
{"id": "PMID:486289", "title": "Changes in cardiac glycoside receptor sites, 86rubidium uptake and intracellular sodium concentrations in the erythrocytes of patients receiving digoxin during the early phases of treatment of cardiac failure in regular rhythm and of atrial fibrillation.", "content": "1 Measurements of the binding of 12-alpha-[3H]-digoxin to the membranes of intact erythrocytes, erythrocytic 86rubidium uptake and intraerythrocytic sodium concentrations have been made in the red cells of patients receiving digoxin in the short-term for atrial fibrillation or cardiac failure in regular rhythm. 2 During the first few days of treatment [3H]-digoxin binding and 86rubidium uptake fall and intraerythrocytic sodium concentrations rise. 3 Subsequently parallel fluctuations occur in [3H]-digoxin binding and 86rubidium uptake but not in intraerythrocytic sodium concentrations and the significance of the fluctuations is discussed. 4 The values of all three measurements correlate significantly with the response of the heart in sinus rhythm as measured by QS2I. 5 Plasma digoxin concentrations do not correlate with QS2I.", "contents": "Changes in cardiac glycoside receptor sites, 86rubidium uptake and intracellular sodium concentrations in the erythrocytes of patients receiving digoxin during the early phases of treatment of cardiac failure in regular rhythm and of atrial fibrillation. 1 Measurements of the binding of 12-alpha-[3H]-digoxin to the membranes of intact erythrocytes, erythrocytic 86rubidium uptake and intraerythrocytic sodium concentrations have been made in the red cells of patients receiving digoxin in the short-term for atrial fibrillation or cardiac failure in regular rhythm. 2 During the first few days of treatment [3H]-digoxin binding and 86rubidium uptake fall and intraerythrocytic sodium concentrations rise. 3 Subsequently parallel fluctuations occur in [3H]-digoxin binding and 86rubidium uptake but not in intraerythrocytic sodium concentrations and the significance of the fluctuations is discussed. 4 The values of all three measurements correlate significantly with the response of the heart in sinus rhythm as measured by QS2I. 5 Plasma digoxin concentrations do not correlate with QS2I."}
{"id": "PMID:486290", "title": "The acute changes seen in cardiac glycoside receptor sites, 86rubidium uptake and intracellular sodium concentrations in the erythrocytes of patients during the early phases of digoxin therapy are not found during chronic therapy: pharmacological and therapeutic implications for chronic digoxin therapy.", "content": "1 Measurements of the binding of 12-alpha-[3H]-digoxin to the membranes of intact erythrocytes, erythrocyte 86rubidium uptake and intraerythrocytic sodium concentrations have been made in the red cells of various groups of patients--those who have not received digoxin, those during the early phases of treatment, those during chronic (greater than 2 months) treatment, and those toxic. 2 The values of those measurements in the patients in the early phases of therapy and in the toxic patients differed significantly from those of the untreated patients. 3 However, the values in the chronically treated patients were not different from those of the untreated patients. 4 The results suggest that the biochemical pharmacological effects of digoxin which occur during the early phases of therapy do not persist in the long-term. 5 The possible clinical significance of these observations is discussed.", "contents": "The acute changes seen in cardiac glycoside receptor sites, 86rubidium uptake and intracellular sodium concentrations in the erythrocytes of patients during the early phases of digoxin therapy are not found during chronic therapy: pharmacological and therapeutic implications for chronic digoxin therapy. 1 Measurements of the binding of 12-alpha-[3H]-digoxin to the membranes of intact erythrocytes, erythrocyte 86rubidium uptake and intraerythrocytic sodium concentrations have been made in the red cells of various groups of patients--those who have not received digoxin, those during the early phases of treatment, those during chronic (greater than 2 months) treatment, and those toxic. 2 The values of those measurements in the patients in the early phases of therapy and in the toxic patients differed significantly from those of the untreated patients. 3 However, the values in the chronically treated patients were not different from those of the untreated patients. 4 The results suggest that the biochemical pharmacological effects of digoxin which occur during the early phases of therapy do not persist in the long-term. 5 The possible clinical significance of these observations is discussed."}
{"id": "PMID:486291", "title": "Plasma concentrations of propranolol and 4-hydroxypropranolol during chronic oral propranolol therapy.", "content": "1 The plasma levels of propranolol and 4-hydroxypropranolol have been measured in 17 hypertensive patients receiving chronic oral therapy with propranolol. 2 The range of plasma propranolol concentrations was from 5.3 to 300 ng/ml, and that of 4-hydroxypropranolol was from 2.1 to 36.0 ng/ml. 3 The mean (+/- s.d.) plasma concentration ratio of 4-hydroxypropranolol to propranolol was 0.130 (+/- 0.005); however, a very wide range was observed with individual values ranging from 0.057 to 0.241. 4 A statistically significant correlation was observed between the plasma concentration of 4-hydroxypropranolol and that of propranolol. 5 Propranolol and 4-hydroxypropranolol plasma concentrations were each significantly, but poorly, correlated with daily propranolol dose. 6 The clinical significance of the results has been discussed.", "contents": "Plasma concentrations of propranolol and 4-hydroxypropranolol during chronic oral propranolol therapy. 1 The plasma levels of propranolol and 4-hydroxypropranolol have been measured in 17 hypertensive patients receiving chronic oral therapy with propranolol. 2 The range of plasma propranolol concentrations was from 5.3 to 300 ng/ml, and that of 4-hydroxypropranolol was from 2.1 to 36.0 ng/ml. 3 The mean (+/- s.d.) plasma concentration ratio of 4-hydroxypropranolol to propranolol was 0.130 (+/- 0.005); however, a very wide range was observed with individual values ranging from 0.057 to 0.241. 4 A statistically significant correlation was observed between the plasma concentration of 4-hydroxypropranolol and that of propranolol. 5 Propranolol and 4-hydroxypropranolol plasma concentrations were each significantly, but poorly, correlated with daily propranolol dose. 6 The clinical significance of the results has been discussed."}
{"id": "PMID:486292", "title": "Determination of viloxazine in plasma by GLC.", "content": "1 A gas chromatographic procedure for the determination of viloxazine in therapeutic concentrations in human plasma, with use of a flame ionization detector, is described. 2 The drug is extracted at pH 12 into hexane, back extracted into HCl, re-extracted into hexane after alkalinization of the HCl and derivatized with acetic anhydride. 3 An imipramine-butriptyline mixture is used as an internal standard. The coefficients of variation for a concentration of 0.75 microgram/ml are 9.4% and 13.1% for within day and day to day precision respectively. For a 1.75 micrograms/ml solution the within day precision was 5.7%. 4 The method has been applied to a patient receiving 300 mg/day viloxazine treatment.", "contents": "Determination of viloxazine in plasma by GLC. 1 A gas chromatographic procedure for the determination of viloxazine in therapeutic concentrations in human plasma, with use of a flame ionization detector, is described. 2 The drug is extracted at pH 12 into hexane, back extracted into HCl, re-extracted into hexane after alkalinization of the HCl and derivatized with acetic anhydride. 3 An imipramine-butriptyline mixture is used as an internal standard. The coefficients of variation for a concentration of 0.75 microgram/ml are 9.4% and 13.1% for within day and day to day precision respectively. For a 1.75 micrograms/ml solution the within day precision was 5.7%. 4 The method has been applied to a patient receiving 300 mg/day viloxazine treatment."}
{"id": "PMID:486293", "title": "Concentration effect studies with oral metoclopramide.", "content": "1 The effects of single oral doses of 10 mg and 20 mg metoclopramide have been compared to placebo in six normal male volunteers. 2 The drug did not significantly increase the rate of gastric emptying as measured by ethanol absorption. 3 Sedation during the absorption of ethanol was only observed 1 h after the 20 mg oral dose of metoclopramide (P less than 0.05). 4. Akathisia, a central nervous system side effect of metoclopramide, occurred only in subjects who had peak plasma concentrations above 100 ng/ml. 5 It has not been possible to define a concentration-effect relationship for the action of metoclopramide on the stomach and comparison with previous results after intravenous dosing suggests that the route of administration is of major importance in determining the action of this drug.", "contents": "Concentration effect studies with oral metoclopramide. 1 The effects of single oral doses of 10 mg and 20 mg metoclopramide have been compared to placebo in six normal male volunteers. 2 The drug did not significantly increase the rate of gastric emptying as measured by ethanol absorption. 3 Sedation during the absorption of ethanol was only observed 1 h after the 20 mg oral dose of metoclopramide (P less than 0.05). 4. Akathisia, a central nervous system side effect of metoclopramide, occurred only in subjects who had peak plasma concentrations above 100 ng/ml. 5 It has not been possible to define a concentration-effect relationship for the action of metoclopramide on the stomach and comparison with previous results after intravenous dosing suggests that the route of administration is of major importance in determining the action of this drug."}
{"id": "PMID:486305", "title": "Distribution of misonidazole in human tumours and normal tissues.", "content": "Levels of misonidazole in human tumours, normal tissues and blood have been measured in patients given a 1g oral dose of drug before surgery or biopsy. The results show that 50--70% of the blood level was found in a wide range of tumours and that similar levels were found in adjacent normal tissues. Good penetration of drug was achieved within tumours, and up to 90--100% of the blood level was found in the necrotic cyst fluid at the centre of some tumours. CSF studies showed free diffusion into the CNS, which was confirmed by finding 50--70% of the blood level within brain tumours. A delay of passage of drugs into the CSF was noted, which was not found for drug diffusion into bile and saliva.", "contents": "Distribution of misonidazole in human tumours and normal tissues. Levels of misonidazole in human tumours, normal tissues and blood have been measured in patients given a 1g oral dose of drug before surgery or biopsy. The results show that 50--70% of the blood level was found in a wide range of tumours and that similar levels were found in adjacent normal tissues. Good penetration of drug was achieved within tumours, and up to 90--100% of the blood level was found in the necrotic cyst fluid at the centre of some tumours. CSF studies showed free diffusion into the CNS, which was confirmed by finding 50--70% of the blood level within brain tumours. A delay of passage of drugs into the CSF was noted, which was not found for drug diffusion into bile and saliva."}
{"id": "PMID:486306", "title": "Misonidazole and MTDQ in combination: cytotoxic and radiosensitizing properties in hypoxic mammalian cells.", "content": "A combination of misonidazole and MTDQ (6,6'-methylene-bis-2,2,4 trimethyl-1,2-dihydroquinoline) has been tested for its radiation-sensitizing properties and cytotoxicity, using Chinese hamster V79 cells cultured in vitro. Both compounds sensitize hypoxic cells to the effects of X-rays, and when used in combination their sensitizing properties are additive. By contrast, the presence of MTDQ completely inhibits the cytotoxicity that misonidazole exhibits towards hypoxic cells. These experiments shed some light on the mechanism of action of electron-affinic hypoxic cell sensitizers, and the combination of radiosensitizers suggested may have an application in human cancer radiotherapy by eliminating the neurotoxicity experienced by patients receiving misonidazole during radiotherapy.", "contents": "Misonidazole and MTDQ in combination: cytotoxic and radiosensitizing properties in hypoxic mammalian cells. A combination of misonidazole and MTDQ (6,6'-methylene-bis-2,2,4 trimethyl-1,2-dihydroquinoline) has been tested for its radiation-sensitizing properties and cytotoxicity, using Chinese hamster V79 cells cultured in vitro. Both compounds sensitize hypoxic cells to the effects of X-rays, and when used in combination their sensitizing properties are additive. By contrast, the presence of MTDQ completely inhibits the cytotoxicity that misonidazole exhibits towards hypoxic cells. These experiments shed some light on the mechanism of action of electron-affinic hypoxic cell sensitizers, and the combination of radiosensitizers suggested may have an application in human cancer radiotherapy by eliminating the neurotoxicity experienced by patients receiving misonidazole during radiotherapy."}
{"id": "PMID:486307", "title": "ICRF-159 enhancement of radiation response in combined modality therapies. I. Time/dose relationships for tumour response.", "content": "The combined effect of the chemotherapeutic agent ICRF-159 and irradiation were evaluated using the Lewis lung tumour (LL). At a daily dose of 25 mg/kg, ICOF given alone prevented the progressive growth of LL. Daily pretreatment also potentiated the effects of radiation (600 rad) on tumour growth, provided the pretreatment kinetics of the tumour permitted a response to radiation alone. Single acute doses of the drug failed to alter the growth of LL, and when combined with radiation failed to enhance the radiation effect. Fractionation of the drug (25 mg/kg; 4 doses at 3h intervals) before irradiation, however, results in immediate effects on tumour growth which are more than additive. The results suggest that a low dose of ICRF-159 for extended periods is more effective in enhancing radiotherapy than a high dose provided acutely.", "contents": "ICRF-159 enhancement of radiation response in combined modality therapies. I. Time/dose relationships for tumour response. The combined effect of the chemotherapeutic agent ICRF-159 and irradiation were evaluated using the Lewis lung tumour (LL). At a daily dose of 25 mg/kg, ICOF given alone prevented the progressive growth of LL. Daily pretreatment also potentiated the effects of radiation (600 rad) on tumour growth, provided the pretreatment kinetics of the tumour permitted a response to radiation alone. Single acute doses of the drug failed to alter the growth of LL, and when combined with radiation failed to enhance the radiation effect. Fractionation of the drug (25 mg/kg; 4 doses at 3h intervals) before irradiation, however, results in immediate effects on tumour growth which are more than additive. The results suggest that a low dose of ICRF-159 for extended periods is more effective in enhancing radiotherapy than a high dose provided acutely."}
{"id": "PMID:486308", "title": "ICRF-159 enhancement of radiation response in combined modality therapies. II. Differential responses of tumour and normal tissues.", "content": "The combined effect of the chemotherapeutic agent ICRF-159 and radiation on the proliferative status of tumor/normal systems has been evaluated using the Lewis lung tumour in BDF1 mice. We have previously shown that a 25 mg/kg dose of ICRF-159, given at 3h intervals X4 before irradiation, significantly enhanced tumour growth retardation relative to a single dose of 100 mg/kg before irradiation. Whilst both single and fractionated drug treatments produced a transient inhibition of cell proliferation, comparisons of the temporal recovery from the antiproliferative effect of radiation in both tumour and intestinal epithelium suggested that single acute doses of ICRF-159 fail to potentiate the radiation response of either tissue. Protracted drug administration before irradiation, however, markedly decreases the post-radiation proliferative recovery of the tumour, without significantly altering intestinal recovery. The data suggest that both drug concentration and/or exposure time determine the interactions seen with combined modes.", "contents": "ICRF-159 enhancement of radiation response in combined modality therapies. II. Differential responses of tumour and normal tissues. The combined effect of the chemotherapeutic agent ICRF-159 and radiation on the proliferative status of tumor/normal systems has been evaluated using the Lewis lung tumour in BDF1 mice. We have previously shown that a 25 mg/kg dose of ICRF-159, given at 3h intervals X4 before irradiation, significantly enhanced tumour growth retardation relative to a single dose of 100 mg/kg before irradiation. Whilst both single and fractionated drug treatments produced a transient inhibition of cell proliferation, comparisons of the temporal recovery from the antiproliferative effect of radiation in both tumour and intestinal epithelium suggested that single acute doses of ICRF-159 fail to potentiate the radiation response of either tissue. Protracted drug administration before irradiation, however, markedly decreases the post-radiation proliferative recovery of the tumour, without significantly altering intestinal recovery. The data suggest that both drug concentration and/or exposure time determine the interactions seen with combined modes."}
{"id": "PMID:486309", "title": "25-Hydroxycholecalciferol receptors in human breast cancer.", "content": "Cytosol receptors for 25-hydroxycholecalciferol, oestradiol and progesterone were measured in human mammary carcinomas. Significant positive correlations were found between the concentrations of all three receptors.", "contents": "25-Hydroxycholecalciferol receptors in human breast cancer. Cytosol receptors for 25-hydroxycholecalciferol, oestradiol and progesterone were measured in human mammary carcinomas. Significant positive correlations were found between the concentrations of all three receptors."}
{"id": "PMID:486310", "title": "Elastosis and response to endocrine therapy in human breast cancer.", "content": "Response to endocrine therapy in 51 patients with advanced breast cancer was compared with the amount of elastosis in histological sections from their primary tumours. There appeared to be an association between elastosis and response: tumours with no elastosis showed a lower rate of response than those with gross elastosis, indicating that this simple method might provide a useful predictive index for response to endocrine therapy. In addition, tumours with oestrogen-receptor activity (a feature associated with a high rate of response) but with no elastosis were unlikely to respond, suggesting that a combination of the 2 predictive indices might be more valuable than either taken alone.", "contents": "Elastosis and response to endocrine therapy in human breast cancer. Response to endocrine therapy in 51 patients with advanced breast cancer was compared with the amount of elastosis in histological sections from their primary tumours. There appeared to be an association between elastosis and response: tumours with no elastosis showed a lower rate of response than those with gross elastosis, indicating that this simple method might provide a useful predictive index for response to endocrine therapy. In addition, tumours with oestrogen-receptor activity (a feature associated with a high rate of response) but with no elastosis were unlikely to respond, suggesting that a combination of the 2 predictive indices might be more valuable than either taken alone."}
{"id": "PMID:486311", "title": "Evidence for serum binding of oxidized spermine and its potent G1-phase inhibition of cell proliferation.", "content": "Serum polyamine oxidase (EC 1.4.3.4) is known to react in vitro with radio-labelled spermine4+ to produce di-oxidized spermine which must incorporate the label. Di-oxidized spermine was compatible with a radio-labelled compound2+ separated from the reaction mixture by ion-exchange chromatography. The compound was measured and had a half-life of about 2.3 h in tissue culture medium. It also rapidly and tightly bound to an unidentified serum component (gel-filtration chromatography indicated a complex of mol. wt 70,000) so that dissociation required treatment with strong acid (10N HCl). Findings suggest that the di-oxidized spermine, in either its free cationic or bound form, potently arrested cell proliferation. This arrest was non-cytotoxic and was confined to the G1 phase of the cell cycle. Products of di-oxidized spermine autodegradation, including trace amounts of stable and cytotoxic acrolein (arrested S phase), were unlikely to have contributed significantly to the arrest.", "contents": "Evidence for serum binding of oxidized spermine and its potent G1-phase inhibition of cell proliferation. Serum polyamine oxidase (EC 1.4.3.4) is known to react in vitro with radio-labelled spermine4+ to produce di-oxidized spermine which must incorporate the label. Di-oxidized spermine was compatible with a radio-labelled compound2+ separated from the reaction mixture by ion-exchange chromatography. The compound was measured and had a half-life of about 2.3 h in tissue culture medium. It also rapidly and tightly bound to an unidentified serum component (gel-filtration chromatography indicated a complex of mol. wt 70,000) so that dissociation required treatment with strong acid (10N HCl). Findings suggest that the di-oxidized spermine, in either its free cationic or bound form, potently arrested cell proliferation. This arrest was non-cytotoxic and was confined to the G1 phase of the cell cycle. Products of di-oxidized spermine autodegradation, including trace amounts of stable and cytotoxic acrolein (arrested S phase), were unlikely to have contributed significantly to the arrest."}
{"id": "PMID:486312", "title": "Monocytes and macrophages in malignant melanoma IV. Effects of C. parvum on monocyte function.", "content": "Assays for the capacity of peripheral-blood monocytes (a) to mature in vitro into macrophages, (b) to reduce nitro-blue tetrazolium (NBT) and (c) to lyse antibody-coated human Group A red cells, were applied to a group of 82 patients with histologically proven malignant melanoma. In patients with micrometastatic disease there was an enhancement of red-cell lysis and NBT reduction, suggesting that their monocytes are in some way \"activated\", whereas NBT reduction was suppressed in those with overt dissemination. Monocyte maturation in vitro was impaired in all patient groups to an extent which correlated with overall tumour burden. Corynebacterium parvum was administered i.v. to 12 patients with disseminated disease and by the intradermal route to 24 patients with micrometastatic disease. The 3 monocyte functions were significantly enhanced by C. parvum.", "contents": "Monocytes and macrophages in malignant melanoma IV. Effects of C. parvum on monocyte function. Assays for the capacity of peripheral-blood monocytes (a) to mature in vitro into macrophages, (b) to reduce nitro-blue tetrazolium (NBT) and (c) to lyse antibody-coated human Group A red cells, were applied to a group of 82 patients with histologically proven malignant melanoma. In patients with micrometastatic disease there was an enhancement of red-cell lysis and NBT reduction, suggesting that their monocytes are in some way \"activated\", whereas NBT reduction was suppressed in those with overt dissemination. Monocyte maturation in vitro was impaired in all patient groups to an extent which correlated with overall tumour burden. Corynebacterium parvum was administered i.v. to 12 patients with disseminated disease and by the intradermal route to 24 patients with micrometastatic disease. The 3 monocyte functions were significantly enhanced by C. parvum."}
{"id": "PMID:486313", "title": "An approach to the problem of heterogeneity of human tumour-cell populations.", "content": "1. Successive sampling of ovarian cancers during cytostatic treatment showed several cases of notable changes in their ploidy distribution and one change in model chromosome number, indicating selection of a resistant tumour-cell population. 2. Studies of cell suspensions from human tumour specimens incubated with [3H]-TdR after exposure in vitro to various cytostatic agents have shown variation in labelling between different parts of the same tumour, as well as between the primary tumour and its metastases or ascitic tumour-cell population, which may be accounted for by variation in sensitivity of the tumour-cell population. 3. Studies of nuclear morphology in 20 endometrial cancers before and after progesterone therapy demonstrate considerable variation in the proportion of cells undergoing secretory conversion within the same tumour, indicating primary heterogeneity of the tumour-cell population in response to progesterone.", "contents": "An approach to the problem of heterogeneity of human tumour-cell populations. 1. Successive sampling of ovarian cancers during cytostatic treatment showed several cases of notable changes in their ploidy distribution and one change in model chromosome number, indicating selection of a resistant tumour-cell population. 2. Studies of cell suspensions from human tumour specimens incubated with [3H]-TdR after exposure in vitro to various cytostatic agents have shown variation in labelling between different parts of the same tumour, as well as between the primary tumour and its metastases or ascitic tumour-cell population, which may be accounted for by variation in sensitivity of the tumour-cell population. 3. Studies of nuclear morphology in 20 endometrial cancers before and after progesterone therapy demonstrate considerable variation in the proportion of cells undergoing secretory conversion within the same tumour, indicating primary heterogeneity of the tumour-cell population in response to progesterone."}
{"id": "PMID:486319", "title": "Evaluation of leg ulcer treatment with stereophotogrammetry. A pilot study.", "content": "This report describes stereophotogrammetric measurements of leg ulcers. A basic technical description of the method and its application in a pilot study are presented. The clinical evaluation of the healing of leg ulcers is compared to the photogrammetrically determined parameters--edge length, surface area, and volume--of the ulcers, and seems to fulfil the need for an objective method in the evaluation of leg ulcer treatment.", "contents": "Evaluation of leg ulcer treatment with stereophotogrammetry. A pilot study. This report describes stereophotogrammetric measurements of leg ulcers. A basic technical description of the method and its application in a pilot study are presented. The clinical evaluation of the healing of leg ulcers is compared to the photogrammetrically determined parameters--edge length, surface area, and volume--of the ulcers, and seems to fulfil the need for an objective method in the evaluation of leg ulcer treatment."}
{"id": "PMID:486320", "title": "Mepacrine pigmentation in systemic lupus erythematosus. New data from an ultrastructural, biochemical and analytical electron microscopic investigation.", "content": "A case of mepacrine pigmentation occurring in a patient with systemic lupus erythematosus has been investigated by fluorescent light microscopy, gas--liquid chromatography and analytical electron microscopy. There is strong evidence for the presence of mepacrine itself within the typical granules, which have been shown by electron microscopy to be membrane bound and intracellular. Analytical electron microscopy also showed that the granules contain large quantities of iron and smaller quantities of sulphur.", "contents": "Mepacrine pigmentation in systemic lupus erythematosus. New data from an ultrastructural, biochemical and analytical electron microscopic investigation. A case of mepacrine pigmentation occurring in a patient with systemic lupus erythematosus has been investigated by fluorescent light microscopy, gas--liquid chromatography and analytical electron microscopy. There is strong evidence for the presence of mepacrine itself within the typical granules, which have been shown by electron microscopy to be membrane bound and intracellular. Analytical electron microscopy also showed that the granules contain large quantities of iron and smaller quantities of sulphur."}
{"id": "PMID:486321", "title": "Acid hydrolases in blister fluid. 7. Influence of dimethyl sulphoxide and heat.", "content": "Increased acid hydrolase activity has been demonstrated in suction blister fluid 1.5 h after the application of established lysosomal labilizing agents. Both the pattern of enzyme activity and the magnitude of the increases were similar to those following cutaneous inflammation. These data confirm our previous observations that acid hydrolases do indeed play a role in cutaneous inflammation, but their release is not the initial event in this process.", "contents": "Acid hydrolases in blister fluid. 7. Influence of dimethyl sulphoxide and heat. Increased acid hydrolase activity has been demonstrated in suction blister fluid 1.5 h after the application of established lysosomal labilizing agents. Both the pattern of enzyme activity and the magnitude of the increases were similar to those following cutaneous inflammation. These data confirm our previous observations that acid hydrolases do indeed play a role in cutaneous inflammation, but their release is not the initial event in this process."}
{"id": "PMID:486322", "title": "Geographic tongue--a manifestation of atopy.", "content": "One hundred patients with geographic tongue have been studied for evidence of an association with atopy. There was a significantly high frequency of personal and/or family history of asthma, eczema and hay fever amongst the patients when compared to a control population. A raised total serum IgE( greater than or equal to 200 u/ml) was significantly more frequent in patients compared to a control population. These findings confirm the impression that in some patients geographic tongue is a sign of atopy.", "contents": "Geographic tongue--a manifestation of atopy. One hundred patients with geographic tongue have been studied for evidence of an association with atopy. There was a significantly high frequency of personal and/or family history of asthma, eczema and hay fever amongst the patients when compared to a control population. A raised total serum IgE( greater than or equal to 200 u/ml) was significantly more frequent in patients compared to a control population. These findings confirm the impression that in some patients geographic tongue is a sign of atopy."}
{"id": "PMID:486323", "title": "The effects on epidermal DNA synthesis of topically applied cycloheximide.", "content": "An animal model for investigating topically applied epidermal cytotoxic drugs to determine optimum therapeutic doses and schedules is described. It is used here to examine the effects of cycloheximide on epidermal DNA synthesis, as measured by 3H-thymidine incorporation in vivo, in terms of dose/response and activity/time profiles and potential systemic toxicity.", "contents": "The effects on epidermal DNA synthesis of topically applied cycloheximide. An animal model for investigating topically applied epidermal cytotoxic drugs to determine optimum therapeutic doses and schedules is described. It is used here to examine the effects of cycloheximide on epidermal DNA synthesis, as measured by 3H-thymidine incorporation in vivo, in terms of dose/response and activity/time profiles and potential systemic toxicity."}
{"id": "PMID:486324", "title": "The treatment of cutaneous malignancies with topically applied cycloheximide.", "content": "Results obtained from an animal test system for epidermal cytotoxic drugs prompted the topical use of 1% cycloheximide in the treatment of histologically confirmed superficial basal cell carcinomata of the skin. Excellent clinical clearing of lesions has been demonstrated and this has been confirmed by post treatment biopsies. Preliminary findings in solar keratoses and intraepidermal carcinomata of the skin are also reported.", "contents": "The treatment of cutaneous malignancies with topically applied cycloheximide. Results obtained from an animal test system for epidermal cytotoxic drugs prompted the topical use of 1% cycloheximide in the treatment of histologically confirmed superficial basal cell carcinomata of the skin. Excellent clinical clearing of lesions has been demonstrated and this has been confirmed by post treatment biopsies. Preliminary findings in solar keratoses and intraepidermal carcinomata of the skin are also reported."}
{"id": "PMID:486325", "title": "Trichophyton violaceum abscesses.", "content": "Two patients with deep cold abscesses due to Trichophyton violaceum are described. Both have depressed cellular immunity.", "contents": "Trichophyton violaceum abscesses. Two patients with deep cold abscesses due to Trichophyton violaceum are described. Both have depressed cellular immunity."}
{"id": "PMID:486326", "title": "Familial cutaneous collagenoma: genetic studies on a family.", "content": "Familial cutaneous collagenoma is an inherited condition characterized by the presence of multiple dermal nodules symmetrically distributed on the trunk and upper arms. In this study, six patients, the proband, his four siblings and a niece, representing a kindred of fifty-two subjects, were examined for aymptomatic cutaneous nodules mainly on the back and chest. The individual lesions varying from a few millimetres to several centimetres in size, were indurated, and showed minimal epidermal changes. Histologically, the nodules were characterized by an excessive accumulation of dense, coarse collagen fibres in the dermis. The elastic fibres appeared diminished in number, and in some areas they were abnormally thin and fragmented. The lesions, therefore, were connective tissue naevi of the collagen type. On the basis of the family history and histological observations the patients were diagnosed as having familial cutaneous collagenoma. Examination of the family pedigree indicated that the dermal nodules in familial cutaneous collagenoma were inherited in an autosomal dominant pattern. It was also observed that the lesions had an onset at the age of 15 to 19 years, and their number increased significantly during pregnancy. It is conceivable that familial cutaneous collagenoma is an inherited condition whose expression may be under a hormonal control.", "contents": "Familial cutaneous collagenoma: genetic studies on a family. Familial cutaneous collagenoma is an inherited condition characterized by the presence of multiple dermal nodules symmetrically distributed on the trunk and upper arms. In this study, six patients, the proband, his four siblings and a niece, representing a kindred of fifty-two subjects, were examined for aymptomatic cutaneous nodules mainly on the back and chest. The individual lesions varying from a few millimetres to several centimetres in size, were indurated, and showed minimal epidermal changes. Histologically, the nodules were characterized by an excessive accumulation of dense, coarse collagen fibres in the dermis. The elastic fibres appeared diminished in number, and in some areas they were abnormally thin and fragmented. The lesions, therefore, were connective tissue naevi of the collagen type. On the basis of the family history and histological observations the patients were diagnosed as having familial cutaneous collagenoma. Examination of the family pedigree indicated that the dermal nodules in familial cutaneous collagenoma were inherited in an autosomal dominant pattern. It was also observed that the lesions had an onset at the age of 15 to 19 years, and their number increased significantly during pregnancy. It is conceivable that familial cutaneous collagenoma is an inherited condition whose expression may be under a hormonal control."}
{"id": "PMID:486327", "title": "Disseminated dermal melanocytosis terminating in melanoma. A human condition resembling equine melanotic disease.", "content": "A detailed account is given of a progressive, punctate dermal melanocytosis which, commencing in childhood, terminated in the fifth decade as disseminated melanoma with no primary site discoverable. The terminal findings were characterized by prominent melanophagia. In the author's opinion there are resemblances between the unique condition described and a form of equine melanotic disease.", "contents": "Disseminated dermal melanocytosis terminating in melanoma. A human condition resembling equine melanotic disease. A detailed account is given of a progressive, punctate dermal melanocytosis which, commencing in childhood, terminated in the fifth decade as disseminated melanoma with no primary site discoverable. The terminal findings were characterized by prominent melanophagia. In the author's opinion there are resemblances between the unique condition described and a form of equine melanotic disease."}
{"id": "PMID:486328", "title": "Pernicious anaemia and granulomatous skin lesions in a case of common variable hypogammaglobulinaemia.", "content": "A 45-year-old woman is reported with a history of recurrent bacterial infections since childhood; at the age of 37 years she developed recurrent ulcerated lesions around her mouth, in the groins and buttocks, which histologically contained small, non-caseating granulomas. A year latter when being treated for empyema, she was found to have common variable hypogammaglobulinaemia. Three years after this she developed a megaloblastic anaemia of vitamin B12-deficiency type.", "contents": "Pernicious anaemia and granulomatous skin lesions in a case of common variable hypogammaglobulinaemia. A 45-year-old woman is reported with a history of recurrent bacterial infections since childhood; at the age of 37 years she developed recurrent ulcerated lesions around her mouth, in the groins and buttocks, which histologically contained small, non-caseating granulomas. A year latter when being treated for empyema, she was found to have common variable hypogammaglobulinaemia. Three years after this she developed a megaloblastic anaemia of vitamin B12-deficiency type."}
{"id": "PMID:486338", "title": "A comparison of predictions from peripheral and central theories of emotion.", "content": "Peripheral and central theories of emotion differ in the role they ascribe to cognitive and physiological variables in arousal. This study compared the responses of quadriplegics and normal controls to sexually arousing stimuli under various conditions of false heart-rate feedback. The results support a centralist position and the relatively greater importance of cognitive factors in emotional responsiveness.", "contents": "A comparison of predictions from peripheral and central theories of emotion. Peripheral and central theories of emotion differ in the role they ascribe to cognitive and physiological variables in arousal. This study compared the responses of quadriplegics and normal controls to sexually arousing stimuli under various conditions of false heart-rate feedback. The results support a centralist position and the relatively greater importance of cognitive factors in emotional responsiveness."}
{"id": "PMID:486339", "title": "Clinical responses of A and B practising psychotherapists to schizoid and neurotic patient prototypes.", "content": "Eighty-three male psychotherapists varying on A-B type and experience orally responded to tape-recordings of simulated schizoid and neurotic patients in the privacy of their own offices. Five dimensions of therapist response were studied: accurate empathy, positive social-emotional reactions, negative social-emotional reactions, length of response and proportion of declarative statements. Although the simple A-B interaction effect was not found, significant second-order interactions were found for both accurate empathy and positive reactions which indicated that the predicted interaction effect tends to be upheld for inexperienced therapists but attenuated or reversed for experienced therapists. A significantly reversed interaction for experienced therapists' response length was also found. Examination of the results from this study and previous studies suggested a revision of the A-B interaction hypothesis: for inexperienced therapists, As will be more effective with schizoids/schizophrenics than Bs, whereas Bs will surpass As with neurotics; for experienced therapists, however, this interaction will be attenuated or reversed. Speculations about underlying processes in A-B interaction phenomena were offered.", "contents": "Clinical responses of A and B practising psychotherapists to schizoid and neurotic patient prototypes. Eighty-three male psychotherapists varying on A-B type and experience orally responded to tape-recordings of simulated schizoid and neurotic patients in the privacy of their own offices. Five dimensions of therapist response were studied: accurate empathy, positive social-emotional reactions, negative social-emotional reactions, length of response and proportion of declarative statements. Although the simple A-B interaction effect was not found, significant second-order interactions were found for both accurate empathy and positive reactions which indicated that the predicted interaction effect tends to be upheld for inexperienced therapists but attenuated or reversed for experienced therapists. A significantly reversed interaction for experienced therapists' response length was also found. Examination of the results from this study and previous studies suggested a revision of the A-B interaction hypothesis: for inexperienced therapists, As will be more effective with schizoids/schizophrenics than Bs, whereas Bs will surpass As with neurotics; for experienced therapists, however, this interaction will be attenuated or reversed. Speculations about underlying processes in A-B interaction phenomena were offered."}
{"id": "PMID:486340", "title": "The patient's view of therapy.", "content": "Recent research has suggested an increasing rapprochement between behaviour therapy and psychotherapy, especially in terms of the patient's perceptions. Two hypotheses were tested in this study - (1) that patients would report 'relationship' or 'non-specific' factors to be more useful in treatment than behavioural or psychotherapeutic activities, and (2) that there would be no difference between patients who had received behaviour therapy and patients who had received psychotherapy with respect to the rated importance of non-specific, relationship-type activities. Forty-nine patients who had received either behaviour therapy or psychotherapy were asked to complete in retrospect a questionnaire relating to aspects of therapy which they found useful and 37 (76 per cent) completed questionnaires were returned. Both hypotheses were accepted, and the findings are now discussed in relation to the growing realization of the importance of 'non-specific' factors in therapy.", "contents": "The patient's view of therapy. Recent research has suggested an increasing rapprochement between behaviour therapy and psychotherapy, especially in terms of the patient's perceptions. Two hypotheses were tested in this study - (1) that patients would report 'relationship' or 'non-specific' factors to be more useful in treatment than behavioural or psychotherapeutic activities, and (2) that there would be no difference between patients who had received behaviour therapy and patients who had received psychotherapy with respect to the rated importance of non-specific, relationship-type activities. Forty-nine patients who had received either behaviour therapy or psychotherapy were asked to complete in retrospect a questionnaire relating to aspects of therapy which they found useful and 37 (76 per cent) completed questionnaires were returned. Both hypotheses were accepted, and the findings are now discussed in relation to the growing realization of the importance of 'non-specific' factors in therapy."}
{"id": "PMID:486343", "title": "An analysis of contemporary East African folk psychotherapy.", "content": "In an effort to identify the characteristics of folk psychotherapy that could account for its tenacity in East Africa, a total of 31 Tanzanian shamans were studied. It was found that patients with emotional problems make use of both the folk and Western therapists and that a clear conceptual distinction is made between the services offered. The range of techniques used, style of service delivered, and the fundamental (or underlying) model of causation were all analysed. It was concluded that folk therapy is an effective approach to psychosocial disorders which, in the future, should be considered a dynamic supplement to Western practices. Additionally, based on the appeal of the medicine man, new directions for Western psychotherapy were suggested.", "contents": "An analysis of contemporary East African folk psychotherapy. In an effort to identify the characteristics of folk psychotherapy that could account for its tenacity in East Africa, a total of 31 Tanzanian shamans were studied. It was found that patients with emotional problems make use of both the folk and Western therapists and that a clear conceptual distinction is made between the services offered. The range of techniques used, style of service delivered, and the fundamental (or underlying) model of causation were all analysed. It was concluded that folk therapy is an effective approach to psychosocial disorders which, in the future, should be considered a dynamic supplement to Western practices. Additionally, based on the appeal of the medicine man, new directions for Western psychotherapy were suggested."}
{"id": "PMID:486345", "title": "Exhibitionism - a biological hypothesis.", "content": "Twenty-four male genital exhibitionists were interviewed in detail and followed up for periods of from 1 to 5 years. Biographical, attitudinal and behavioural data obtained from them were examined with a view to providing information about the aetiology of this behaviour. The evidence presented and discussed is consistent with the hypothesis that exhibiting represents the expression of an innate disposition. Exhibitionism is found to be widespread and patterned in form, to have a driven quality and to coexist with a variety of personality types. This hypothesis is further developed by discussion of anthropological and ethological data.", "contents": "Exhibitionism - a biological hypothesis. Twenty-four male genital exhibitionists were interviewed in detail and followed up for periods of from 1 to 5 years. Biographical, attitudinal and behavioural data obtained from them were examined with a view to providing information about the aetiology of this behaviour. The evidence presented and discussed is consistent with the hypothesis that exhibiting represents the expression of an innate disposition. Exhibitionism is found to be widespread and patterned in form, to have a driven quality and to coexist with a variety of personality types. This hypothesis is further developed by discussion of anthropological and ethological data."}
{"id": "PMID:486346", "title": "Panic-fear research in asthma and the nuclear conflict theory of asthma; similarities, differences and clinical implications.", "content": "Recent research with the MMPI panic-fear scale has identified personality traits implicated in the psychological maintenance of the medical intractability of asthma. Intensity of prescribed medication, length of hospitalization, and rates of rehospitalization have been found to relate to MMPI panic-fear scores independent of the objective medical severity of the illness as indexed by longitudinal pulmonary functions. In the present study, MMPI panic-fear scores are related to separation and protection conflicts arising in childhood. While the nuclear conflict theory of asthma maintains that such conflicts occur in nearly all asthmatics and form a genetic component of the asthma, the present study finds that childhood separation and protection conflicts occur in a minority of patients, but may be instrumental in maintaining the medical intractability of the illness.", "contents": "Panic-fear research in asthma and the nuclear conflict theory of asthma; similarities, differences and clinical implications. Recent research with the MMPI panic-fear scale has identified personality traits implicated in the psychological maintenance of the medical intractability of asthma. Intensity of prescribed medication, length of hospitalization, and rates of rehospitalization have been found to relate to MMPI panic-fear scores independent of the objective medical severity of the illness as indexed by longitudinal pulmonary functions. In the present study, MMPI panic-fear scores are related to separation and protection conflicts arising in childhood. While the nuclear conflict theory of asthma maintains that such conflicts occur in nearly all asthmatics and form a genetic component of the asthma, the present study finds that childhood separation and protection conflicts occur in a minority of patients, but may be instrumental in maintaining the medical intractability of the illness."}
{"id": "PMID:486347", "title": "Overt and covert personality traits associated with coronary heart disease.", "content": "Some authors suggested the existence of contradictory traits in the personality pattern associated with coronary heart disease: while presenting overt, active, adult-like traits, coronary subjects would be, at a more covert or repressed level, characterized by passive and infantile tendencies. To test this hypothesis, coronary and control subjects were submitted to three types of personality questionnaire, each of them measuring the same four personality traits (seclusion, impulsiveness, dependence and passivity) which, in the adult individual, are considered by Murray's (1938) theory of personality as persisting from infancy. No difference appeared between the two groups on type 1 questionnaires, describing behavioural features of individuals outwardly displaying the four traits. On type 2 questionnaires, describing tastes and similar areas less subject to social norms, coronary subjects revealed themselves higher than control subjects for passivity (P less than 0.05). and dependence (P less than 0.05). Similarly, they were higher for passivity (P less than 0.05), dependence (P less than 0.001) and impulsiveness (P less than 0.05) on type 3 questionnaires describing symptoms expected to occur occasionally among adults having such traits. Supplementary scales also showed coronary subjects to be more ego-defensive (P less than 0.001) and more self-assertive (P less than 0.05) than controls. These data were considered as supporting the hypothesis.", "contents": "Overt and covert personality traits associated with coronary heart disease. Some authors suggested the existence of contradictory traits in the personality pattern associated with coronary heart disease: while presenting overt, active, adult-like traits, coronary subjects would be, at a more covert or repressed level, characterized by passive and infantile tendencies. To test this hypothesis, coronary and control subjects were submitted to three types of personality questionnaire, each of them measuring the same four personality traits (seclusion, impulsiveness, dependence and passivity) which, in the adult individual, are considered by Murray's (1938) theory of personality as persisting from infancy. No difference appeared between the two groups on type 1 questionnaires, describing behavioural features of individuals outwardly displaying the four traits. On type 2 questionnaires, describing tastes and similar areas less subject to social norms, coronary subjects revealed themselves higher than control subjects for passivity (P less than 0.05). and dependence (P less than 0.05). Similarly, they were higher for passivity (P less than 0.05), dependence (P less than 0.001) and impulsiveness (P less than 0.05) on type 3 questionnaires describing symptoms expected to occur occasionally among adults having such traits. Supplementary scales also showed coronary subjects to be more ego-defensive (P less than 0.001) and more self-assertive (P less than 0.05) than controls. These data were considered as supporting the hypothesis."}
{"id": "PMID:486348", "title": "Orality and smoking.", "content": "This study was designed to investigate the relation of orality and smoking, smoking motivation and anticipatory pleasure in smoking. Ninety-seven male and female subjects completed questionnaires designed to elicit information about their motivation and behaviour. Results indicated that difficulty in stopping smoking was positively related to three non-nutriment oral preoccupations. Smoking for psychological as opposed to social reasons was positively related to two-nutriment oral preoccupations. Anticipation of pleasure in smoking before becoming a smoker was negatively related to nutriment orality, positively related to smoking because of a craving, and, for females only, positively to one type of non-nutriment orality. The results are discussed in relation to the role of oral ingestion and the reaction of the central nervous system to the drug.", "contents": "Orality and smoking. This study was designed to investigate the relation of orality and smoking, smoking motivation and anticipatory pleasure in smoking. Ninety-seven male and female subjects completed questionnaires designed to elicit information about their motivation and behaviour. Results indicated that difficulty in stopping smoking was positively related to three non-nutriment oral preoccupations. Smoking for psychological as opposed to social reasons was positively related to two-nutriment oral preoccupations. Anticipation of pleasure in smoking before becoming a smoker was negatively related to nutriment orality, positively related to smoking because of a craving, and, for females only, positively to one type of non-nutriment orality. The results are discussed in relation to the role of oral ingestion and the reaction of the central nervous system to the drug."}
{"id": "PMID:486349", "title": "Techniques of economic analysis in psychiatric practice.", "content": "Since there is a limit to the amount of money that society can and will spend on health care, economic analysis is becoming increasingly important. This article attempts to review the use that has been made of economic analysis in psychiatric practice. It has been shown that treatment costs are a small part of the total cost of mental ill health, although 'expensive treatments' may reduce the cost of illness to society. In the USA the treatment of schizophrenia has been studied from the economic viewpoint. The economics of the alternatives of hospital and community care have been investigated. Cost-benefit analysis has been modified for application to the monitoring and assessment of psychiatric treatment. Economic analysis can and should be used, in conjunction with clinical analysis, to determine policies for better mental health care.", "contents": "Techniques of economic analysis in psychiatric practice. Since there is a limit to the amount of money that society can and will spend on health care, economic analysis is becoming increasingly important. This article attempts to review the use that has been made of economic analysis in psychiatric practice. It has been shown that treatment costs are a small part of the total cost of mental ill health, although 'expensive treatments' may reduce the cost of illness to society. In the USA the treatment of schizophrenia has been studied from the economic viewpoint. The economics of the alternatives of hospital and community care have been investigated. Cost-benefit analysis has been modified for application to the monitoring and assessment of psychiatric treatment. Economic analysis can and should be used, in conjunction with clinical analysis, to determine policies for better mental health care."}
{"id": "PMID:486350", "title": "The specificity of schizophrenic thought disorder.", "content": "Bannister and Salmon (1966), in a repertory grid study, reported that 'thought-disordered schizophrenics lost significantly more reliability and social agreement when shifted from object to people construing than normal'. This conclusion is of doubtful validity, since the between-grids variable, people vs. objects, was confounded with at least five other variables of possible relevance, three of which have been found by subsequent workers (McPherson & Buckley, 1970; Williams, 1971; Heather, 1976) to affect performance on grids. The main aim of the present experiment was to determine the effects of as many as possible of these variables. The subsidiary aim was to replicate, or otherwise, the findings of Frith & Lillie (1972) and Haynes & Phillips (1973) that the effective discriminator between thought-disorder schizophrenics and other groups on repertory grid tests is not intensity of relationship between constructs but pure (element or internal) consistency. Six grids involving all feasible combinations of rating familiar and unfamiliar people and objects on psychological and physical constructs were administered to 10 thought-disordered schizophrenics, 10 non-thought-disordered schizophrenics and 10 normals. As regards the main aim of the experiment it was found that there was no significant grids x diagnoses interaction on pure consistency, but that there was one on intensity, even with pure consistency partialled out. Taking previous work into consideration, it appears that this effect is weak, inconsistent from experiment to experiment, and anomalous in the present study. As regards the subsidiary aim, the findings of the two previous experiments cited above were confirmed. The discrepant results of McPherson et al. (1973) are considered, and it is noted that they held only for a subsample of the schizophrenic group in that experiment, the results for the total sample of schizophrenics, manics and depressives not being inconsistent with those of other experiments. The theoretical and practical implications of these and previous finding are indicated.", "contents": "The specificity of schizophrenic thought disorder. Bannister and Salmon (1966), in a repertory grid study, reported that 'thought-disordered schizophrenics lost significantly more reliability and social agreement when shifted from object to people construing than normal'. This conclusion is of doubtful validity, since the between-grids variable, people vs. objects, was confounded with at least five other variables of possible relevance, three of which have been found by subsequent workers (McPherson & Buckley, 1970; Williams, 1971; Heather, 1976) to affect performance on grids. The main aim of the present experiment was to determine the effects of as many as possible of these variables. The subsidiary aim was to replicate, or otherwise, the findings of Frith & Lillie (1972) and Haynes & Phillips (1973) that the effective discriminator between thought-disorder schizophrenics and other groups on repertory grid tests is not intensity of relationship between constructs but pure (element or internal) consistency. Six grids involving all feasible combinations of rating familiar and unfamiliar people and objects on psychological and physical constructs were administered to 10 thought-disordered schizophrenics, 10 non-thought-disordered schizophrenics and 10 normals. As regards the main aim of the experiment it was found that there was no significant grids x diagnoses interaction on pure consistency, but that there was one on intensity, even with pure consistency partialled out. Taking previous work into consideration, it appears that this effect is weak, inconsistent from experiment to experiment, and anomalous in the present study. As regards the subsidiary aim, the findings of the two previous experiments cited above were confirmed. The discrepant results of McPherson et al. (1973) are considered, and it is noted that they held only for a subsample of the schizophrenic group in that experiment, the results for the total sample of schizophrenics, manics and depressives not being inconsistent with those of other experiments. The theoretical and practical implications of these and previous finding are indicated."}
{"id": "PMID:486351", "title": "Therapist A--B score and treatment outcome with psychiatric in patients: a table of random numbers.", "content": "The study evaluated the relationship between therapist personality and treatment success for 141 schizophrenics and 94 non-schizophrenics randomly assigned to short or long hospitalization. The 47 therapists were self-rated on the Whitehorn--Betz A--B scale in both the original and the Campbell versions. Outcome was assessed on the Health--Sickness Rating Scale and the Psychiatric Evaluation Form at discharge and at 1 and 2 year follow-up. The hypothesis of greater success for A--type therapists with schizophrenics was not confirmed, nor were alternative relationships evident in the data.", "contents": "Therapist A--B score and treatment outcome with psychiatric in patients: a table of random numbers. The study evaluated the relationship between therapist personality and treatment success for 141 schizophrenics and 94 non-schizophrenics randomly assigned to short or long hospitalization. The 47 therapists were self-rated on the Whitehorn--Betz A--B scale in both the original and the Campbell versions. Outcome was assessed on the Health--Sickness Rating Scale and the Psychiatric Evaluation Form at discharge and at 1 and 2 year follow-up. The hypothesis of greater success for A--type therapists with schizophrenics was not confirmed, nor were alternative relationships evident in the data."}
{"id": "PMID:486353", "title": "Effects of anxiety on attitudes--a semantic differential study.", "content": "Eighteen female out-patients suffering from chronic anxiety states and 25 normal women matched for age filled in an anxiety inventory and completed semantic differential forms on 21 concepts grouped into four areas: characters, locations, human interactions and abstractions. Each concept was rated on 15 scales grouped into four types: evaluation, activity-potency, sex evaluation and danger. The normal subjects were divided into high anxiety and low anxiety subgroups on the basis of their trait anxiety scores. Anxious patients rated themselves with lower self-esteem and confidence. They reacted with apprehension to unfamiliar locations and perceived danger in other people. Sexual activity and the human body were viewed negatively. Anxious patients' apprehension were particularly raised by vague abstractions.", "contents": "Effects of anxiety on attitudes--a semantic differential study. Eighteen female out-patients suffering from chronic anxiety states and 25 normal women matched for age filled in an anxiety inventory and completed semantic differential forms on 21 concepts grouped into four areas: characters, locations, human interactions and abstractions. Each concept was rated on 15 scales grouped into four types: evaluation, activity-potency, sex evaluation and danger. The normal subjects were divided into high anxiety and low anxiety subgroups on the basis of their trait anxiety scores. Anxious patients rated themselves with lower self-esteem and confidence. They reacted with apprehension to unfamiliar locations and perceived danger in other people. Sexual activity and the human body were viewed negatively. Anxious patients' apprehension were particularly raised by vague abstractions."}
{"id": "PMID:486355", "title": "Are there different types of neurotic depression?", "content": "The classification of depression remains controversial (Kendell, 1976). Overall et al. (1966) used the Brief Psychiatric Rating Scale (PBRS), a computer classification procedure and derived three depressed groups: anxious, hostile and retarded depressives. Paykel (1971, 1972) reported four groups derived from a multivariate cluster analysis: psychotic depressives and '...three groups, anxious, hostile and young depressives with personality disorder, from the diversity of patients subsumed under neurotic depression'. Amongst 85 women treated with amitriptyline 'this typology predicted outcome significantly'. This paper presents the results of a cluster analysis of a group of 60 female out-patient neurotic depressives.", "contents": "Are there different types of neurotic depression? The classification of depression remains controversial (Kendell, 1976). Overall et al. (1966) used the Brief Psychiatric Rating Scale (PBRS), a computer classification procedure and derived three depressed groups: anxious, hostile and retarded depressives. Paykel (1971, 1972) reported four groups derived from a multivariate cluster analysis: psychotic depressives and '...three groups, anxious, hostile and young depressives with personality disorder, from the diversity of patients subsumed under neurotic depression'. Amongst 85 women treated with amitriptyline 'this typology predicted outcome significantly'. This paper presents the results of a cluster analysis of a group of 60 female out-patient neurotic depressives."}
{"id": "PMID:486356", "title": "A method of structured brief psychotherapy.", "content": "Structured brief psychotherapy is a method of organizing treatment along problem-solving lines. The features of this method are the selection of problem areas, the translation of insight into specific activity, the structuring of psychodynamics and a concentration on termination events. The method is illustrated by clinical examples.", "contents": "A method of structured brief psychotherapy. Structured brief psychotherapy is a method of organizing treatment along problem-solving lines. The features of this method are the selection of problem areas, the translation of insight into specific activity, the structuring of psychodynamics and a concentration on termination events. The method is illustrated by clinical examples."}
{"id": "PMID:486357", "title": "Problems in a 'creative marriage' following the birth of the first child -- an account of a conjoint marital therapy.", "content": "This description of the marital therapy of a couple treated conjointly by the authors illustrates the profound effects of the couple's childhood experiences of their respective parents' relationships on their own subsequent marriage. The precipitating effect of the birth of the child in the marriage in awakening dormant unconscious conflicts stemming from these early experiences also emerges in the history of the couple and in the material of the therapeutic sessions.", "contents": "Problems in a 'creative marriage' following the birth of the first child -- an account of a conjoint marital therapy. This description of the marital therapy of a couple treated conjointly by the authors illustrates the profound effects of the couple's childhood experiences of their respective parents' relationships on their own subsequent marriage. The precipitating effect of the birth of the child in the marriage in awakening dormant unconscious conflicts stemming from these early experiences also emerges in the history of the couple and in the material of the therapeutic sessions."}
{"id": "PMID:486358", "title": "Verbal conditioning of affect responses of process and reactive schizophrenics in a clinical interview situation.", "content": "Sixteen process and 16 reactive schizoprenics out-patients were compared on a verbal conditioning task in an alternating conditioning-extinction design, using verbal and non-verbal positive social reinforcement to influence the emission of self-referred affect statements. It was found that process subjects failed to condition during the time periods used, while reactives demonstrated a significant trials effect showing trends consistent with those hypothesized from the type of design used. This differential conditionability between groups was shown not to be a function of diagnosis, sex, motivation, severity of illness, medication, hospitalization history, or general speech output. It was concluded that the degree of social responsiveness manifested in the premorbid history of the two groups is also operative in behaviour during the psychotic period, specifically, in responsiveness to positive social reinforcers in a verbal conditioning task.", "contents": "Verbal conditioning of affect responses of process and reactive schizophrenics in a clinical interview situation. Sixteen process and 16 reactive schizoprenics out-patients were compared on a verbal conditioning task in an alternating conditioning-extinction design, using verbal and non-verbal positive social reinforcement to influence the emission of self-referred affect statements. It was found that process subjects failed to condition during the time periods used, while reactives demonstrated a significant trials effect showing trends consistent with those hypothesized from the type of design used. This differential conditionability between groups was shown not to be a function of diagnosis, sex, motivation, severity of illness, medication, hospitalization history, or general speech output. It was concluded that the degree of social responsiveness manifested in the premorbid history of the two groups is also operative in behaviour during the psychotic period, specifically, in responsiveness to positive social reinforcers in a verbal conditioning task."}
{"id": "PMID:486360", "title": "The dietary chaos syndrome: a useful new term?", "content": "Following typical primary anorexia nervosa some subjects enter a state characterized by disordered eating behaviour and emotional instability whilst remaining at or above a normal weight. Other subjects may reach a similar position without having been in a state of anorexia nervosa. Surprisingly the state seems to lack a satisfactory name. The term 'Dietary chaos syndrome' is proposed.", "contents": "The dietary chaos syndrome: a useful new term? Following typical primary anorexia nervosa some subjects enter a state characterized by disordered eating behaviour and emotional instability whilst remaining at or above a normal weight. Other subjects may reach a similar position without having been in a state of anorexia nervosa. Surprisingly the state seems to lack a satisfactory name. The term 'Dietary chaos syndrome' is proposed."}
{"id": "PMID:486361", "title": "Crisis intervention: an experimental study of the effects of a brief period of counselling on the anxiety of relatives of seriously injured or ill hospital patients.", "content": "This study was designed to examine the effects of anxiety levels, as measured by the Gottschalk & Gleser (1969) and the Viney & Westbrook (1976) content analysis scales, of a brief period of supportive counselling of relatives who arrived at a hospital emergency admitting ward with a seriously ill or injured patient. Verbal samples were taken for analysis from the subjects before and after a period of counselling (or a period of no counselling for the control group). The results showed that the initial anxiety levels for subjects in both groups was very high. For both the psychoanalytically oriented Gottschalk & Gleser anxiety scale and the Viney & Westbrook scale of cognitive anxiety there was a decrease in the level of anxiety for the counselled group compared with the non-counselled group. The results showed that such crisis intervention in hospitals for relatives who accompany patients to the hospital can reduce their very high levels of diffuse and generalized anxiety.", "contents": "Crisis intervention: an experimental study of the effects of a brief period of counselling on the anxiety of relatives of seriously injured or ill hospital patients. This study was designed to examine the effects of anxiety levels, as measured by the Gottschalk & Gleser (1969) and the Viney & Westbrook (1976) content analysis scales, of a brief period of supportive counselling of relatives who arrived at a hospital emergency admitting ward with a seriously ill or injured patient. Verbal samples were taken for analysis from the subjects before and after a period of counselling (or a period of no counselling for the control group). The results showed that the initial anxiety levels for subjects in both groups was very high. For both the psychoanalytically oriented Gottschalk & Gleser anxiety scale and the Viney & Westbrook scale of cognitive anxiety there was a decrease in the level of anxiety for the counselled group compared with the non-counselled group. The results showed that such crisis intervention in hospitals for relatives who accompany patients to the hospital can reduce their very high levels of diffuse and generalized anxiety."}
{"id": "PMID:486362", "title": "Intersubject agreement concerning relationships between the positive and negative poles of constructs in repertory grid tests.", "content": "Previous research indicate that there is more intersubject agreement concerning patterns of correlations between constructs than concerning how specific elements are rated on each construct. In this study, Canadian undergraduates (15 women, 15 men) rank-ordered eight photographs of strangers separately on the basis of the positive and negative poles of six constructs. The results indicated that there is more consensus concerning specific patterns of relationships between the positive poles of constructs than concerning those between their negative poles. Some implications of this finding for repertory grid assessment and research were discussed.", "contents": "Intersubject agreement concerning relationships between the positive and negative poles of constructs in repertory grid tests. Previous research indicate that there is more intersubject agreement concerning patterns of correlations between constructs than concerning how specific elements are rated on each construct. In this study, Canadian undergraduates (15 women, 15 men) rank-ordered eight photographs of strangers separately on the basis of the positive and negative poles of six constructs. The results indicated that there is more consensus concerning specific patterns of relationships between the positive poles of constructs than concerning those between their negative poles. Some implications of this finding for repertory grid assessment and research were discussed."}
{"id": "PMID:486363", "title": "Agoraphobic women married to abnormally jealous men.", "content": "Of 36 married female agoraphobics treated by the author over a period of 3 years, seven were married to men who displayed abnormal jealousy. In all these cases the husbands' jealousy adversely influenced their wives' response to treatment, and improvement in wives was associated with increased morbidity in their husbands.", "contents": "Agoraphobic women married to abnormally jealous men. Of 36 married female agoraphobics treated by the author over a period of 3 years, seven were married to men who displayed abnormal jealousy. In all these cases the husbands' jealousy adversely influenced their wives' response to treatment, and improvement in wives was associated with increased morbidity in their husbands."}
{"id": "PMID:486365", "title": "The modification of personality disturbance in a therapeutic community for drug abusers.", "content": "Two personality questionnaires, the MMPI and 16PF, were administered routinely to drug abusers admitted to a newly established hierarchical type of therapeutic community. Questionnaires were repeated at 6 and 12 months with those residents who remained. Comparison of the results with other studies suggests that drug abusers admitted to different treatment centres display a recognizable pattern of personality disturbance, characterized by a combination of neurotic, psychopathic and psychotic elements and an unusual degree of willingness to admit to socially undesirable traits. Twenty-five per cent of residents stayed longer than 6 months. They showed a significant reduction in measured personality disturbance, and at 1 year anxiety was the only outstanding indication of disturbance. The relationship between these results, possible sources of bias and other indices of behaviour change is discussed.", "contents": "The modification of personality disturbance in a therapeutic community for drug abusers. Two personality questionnaires, the MMPI and 16PF, were administered routinely to drug abusers admitted to a newly established hierarchical type of therapeutic community. Questionnaires were repeated at 6 and 12 months with those residents who remained. Comparison of the results with other studies suggests that drug abusers admitted to different treatment centres display a recognizable pattern of personality disturbance, characterized by a combination of neurotic, psychopathic and psychotic elements and an unusual degree of willingness to admit to socially undesirable traits. Twenty-five per cent of residents stayed longer than 6 months. They showed a significant reduction in measured personality disturbance, and at 1 year anxiety was the only outstanding indication of disturbance. The relationship between these results, possible sources of bias and other indices of behaviour change is discussed."}
{"id": "PMID:486367", "title": "An analysis of individual treatment on a token economy for chronic schizophrenic patients.", "content": "Fifty-one individually designed treatment programmes carried out over a 22 month period on a token economy ward for 12 female chronic schizophrenic patients were analysed. Level of withdrawal was found to be a critical factor in patients' response, both in terms of the rate and extent of improvement during the treatment stage, and presence or absence of deterioration during 'weaning' and follow up. No difference in response between type of behavioural problem and methods of treatment or weaning were found.", "contents": "An analysis of individual treatment on a token economy for chronic schizophrenic patients. Fifty-one individually designed treatment programmes carried out over a 22 month period on a token economy ward for 12 female chronic schizophrenic patients were analysed. Level of withdrawal was found to be a critical factor in patients' response, both in terms of the rate and extent of improvement during the treatment stage, and presence or absence of deterioration during 'weaning' and follow up. No difference in response between type of behavioural problem and methods of treatment or weaning were found."}
{"id": "PMID:486368", "title": "Contrasting resources in disturbed and non-disturbed family systems.", "content": "Previous research has shown that problems in family functioning and psychological disturbances in children are closely related. To increase understanding of this complex interaction the present review sets out to make explicit certain aspects of the relationship between child psychopathology and the quality of family life. The usefulness of the concept of resources for analysing major aspects of family functioning (contextual, intra-familial and extra-familial) is discussed within a systems framework and is then applied to a wide range of empirical studies which relate important features of family functioning to disturbed behaviour in children. It is found that interpersonal conflict in the lives of parents before marriage may severely constrain their own psychological development. When the psychological effects of such early disadvantage are amplified by marital conflict other family members are also affected. The notion of resources requires such disabilities to be assessed as well as directing attention to non-family interactions which offer compensating supports.", "contents": "Contrasting resources in disturbed and non-disturbed family systems. Previous research has shown that problems in family functioning and psychological disturbances in children are closely related. To increase understanding of this complex interaction the present review sets out to make explicit certain aspects of the relationship between child psychopathology and the quality of family life. The usefulness of the concept of resources for analysing major aspects of family functioning (contextual, intra-familial and extra-familial) is discussed within a systems framework and is then applied to a wide range of empirical studies which relate important features of family functioning to disturbed behaviour in children. It is found that interpersonal conflict in the lives of parents before marriage may severely constrain their own psychological development. When the psychological effects of such early disadvantage are amplified by marital conflict other family members are also affected. The notion of resources requires such disabilities to be assessed as well as directing attention to non-family interactions which offer compensating supports."}
{"id": "PMID:486369", "title": "Mental hospital experience, classroom instruction and change in conceptions and attitudes towards mental illness.", "content": "Changes in conceptions and attitudes towards mental illness and the mentally ill were investigated in Israel. Questionnaires were presented to 69 student nurses, assessing stereotyped image, authoritarianism, social restrictiveness, benevolence, mental hygiene ideology and interpersonal aetiology before and after psychiatric affiliation in two kinds of mental hospitals, or before and after classroom instruction only. The findings lend cultural generality to previous observations and demonstrate that: (a) classroom instruction is ineffective as an agent of change; (b) practical experience involving personal confrontation with mental patients in a progressive psychiatric hospital, which contradicts the stereotyped image of mental patients and inculcates positive attitudes towards them, results in a change to a more professional scientific orientation and a more humane, accepting and liberal attitude; (c) under hospital conditions which support the stereotyped conception and authoritarian restrictive attitudes, the confrontation will result in preservation, even consolidation and strengthening, of the stereotyped image and undesirable attitudes towards mental patients. The findings were discussed and the implications for training programmes were drawn.", "contents": "Mental hospital experience, classroom instruction and change in conceptions and attitudes towards mental illness. Changes in conceptions and attitudes towards mental illness and the mentally ill were investigated in Israel. Questionnaires were presented to 69 student nurses, assessing stereotyped image, authoritarianism, social restrictiveness, benevolence, mental hygiene ideology and interpersonal aetiology before and after psychiatric affiliation in two kinds of mental hospitals, or before and after classroom instruction only. The findings lend cultural generality to previous observations and demonstrate that: (a) classroom instruction is ineffective as an agent of change; (b) practical experience involving personal confrontation with mental patients in a progressive psychiatric hospital, which contradicts the stereotyped image of mental patients and inculcates positive attitudes towards them, results in a change to a more professional scientific orientation and a more humane, accepting and liberal attitude; (c) under hospital conditions which support the stereotyped conception and authoritarian restrictive attitudes, the confrontation will result in preservation, even consolidation and strengthening, of the stereotyped image and undesirable attitudes towards mental patients. The findings were discussed and the implications for training programmes were drawn."}
{"id": "PMID:486371", "title": "Preferences for behavioural, analytic and gestalt psychotherapy.", "content": "This study investigated preferences for behavioural, analytic and gestalt psychotherapy among a sample of 40 SES class III and IV adult females and 67 college freshmen who had never been actual therapy patients. A scaled survey assessed general preference, preference given an imagined long-standing depressive disorder, preference given an imagined specific phobia, and preference for the therapist-patient relationship. Three audio tapes were designed, each describing one of the modalities. High inter-rater reliability and agreement were determined by three independent judges. Results showed that young females had a general preference for gestalt therapy. Young and old females, but not young males, significantly preferred behavioural therapy for a specific phobia. Under forced-choice conditions the group as a whole significantly preferred gestalt therapy. No differences were found for the relationship or preference given a depressive disorder. Preference was hypothesized as a cognitive structure with potential use in therapist-client matching.", "contents": "Preferences for behavioural, analytic and gestalt psychotherapy. This study investigated preferences for behavioural, analytic and gestalt psychotherapy among a sample of 40 SES class III and IV adult females and 67 college freshmen who had never been actual therapy patients. A scaled survey assessed general preference, preference given an imagined long-standing depressive disorder, preference given an imagined specific phobia, and preference for the therapist-patient relationship. Three audio tapes were designed, each describing one of the modalities. High inter-rater reliability and agreement were determined by three independent judges. Results showed that young females had a general preference for gestalt therapy. Young and old females, but not young males, significantly preferred behavioural therapy for a specific phobia. Under forced-choice conditions the group as a whole significantly preferred gestalt therapy. No differences were found for the relationship or preference given a depressive disorder. Preference was hypothesized as a cognitive structure with potential use in therapist-client matching."}
{"id": "PMID:486372", "title": "Towards a conceptual framework of psychotherapy: a personal view.", "content": "A conceptual view of psychotherapy is put forward for discussion and debate. The view is a personal one based upon experience of life and the practice of psychotherapy over a 10 year period. Harmony between the therapist's personality, the process of therapy and the nature of good human relationships is important. Therapy is seen as a practical discipline and a creative art in which the focus should be upon the patient's existence now and what it could be tomorrow.", "contents": "Towards a conceptual framework of psychotherapy: a personal view. A conceptual view of psychotherapy is put forward for discussion and debate. The view is a personal one based upon experience of life and the practice of psychotherapy over a 10 year period. Harmony between the therapist's personality, the process of therapy and the nature of good human relationships is important. Therapy is seen as a practical discipline and a creative art in which the focus should be upon the patient's existence now and what it could be tomorrow."}
{"id": "PMID:486373", "title": "The observing self and the development of cohesiveness.", "content": "This paper presents a detailed analysis of the movement from fragmentation to rudimentary cohesiveness in the severely disturbed patient. In potentially psychotic patients this progression is necessary before analysis of the narcissistic transferences can occur. A dynamic and behavioural analysis of this progression offers a clear focus for the initial phase of treatment: The development of the observing self.", "contents": "The observing self and the development of cohesiveness. This paper presents a detailed analysis of the movement from fragmentation to rudimentary cohesiveness in the severely disturbed patient. In potentially psychotic patients this progression is necessary before analysis of the narcissistic transferences can occur. A dynamic and behavioural analysis of this progression offers a clear focus for the initial phase of treatment: The development of the observing self."}
{"id": "PMID:486375", "title": "Capgras: case study and reappraisal of psychopathology.", "content": "A case of the Capras phenomenon in a man of 58, arising during the course of a depressive illness, is described. Physical, electroencephalographic and psychometric investigations gave no sign of organic disease, and recovery was complete. Factors leading to the development of a depressive psychosis and its particular manifestation in the Capgras delusion are traced in detail. The psychopathology of the condition and the implications for treatment are discussed.", "contents": "Capgras: case study and reappraisal of psychopathology. A case of the Capras phenomenon in a man of 58, arising during the course of a depressive illness, is described. Physical, electroencephalographic and psychometric investigations gave no sign of organic disease, and recovery was complete. Factors leading to the development of a depressive psychosis and its particular manifestation in the Capgras delusion are traced in detail. The psychopathology of the condition and the implications for treatment are discussed."}
{"id": "PMID:486377", "title": "Arachnoid hyperplasia in optic nerve glioma: confusion with orbital meningioma.", "content": "A case is reported of an optic nerve glioma with a marked degree of arachnoid hyperplasia which was initially diagnosed as an optic nerve meningioma. Hyperplasia of the arachnoid was also the underlying cause for expansion of the optic canal. The relationship between arachnoid hyperplasia in optic nerve glioma and meningioma of the optic nerve sheath in childhood is discussed.", "contents": "Arachnoid hyperplasia in optic nerve glioma: confusion with orbital meningioma. A case is reported of an optic nerve glioma with a marked degree of arachnoid hyperplasia which was initially diagnosed as an optic nerve meningioma. Hyperplasia of the arachnoid was also the underlying cause for expansion of the optic canal. The relationship between arachnoid hyperplasia in optic nerve glioma and meningioma of the optic nerve sheath in childhood is discussed."}
{"id": "PMID:486378", "title": "Clinical presentation and management of lacrimal gland tumours.", "content": "This paper presents the clinical and pathological findings in 40 consecutive patients seen with primary tumours arising from the lacrimal gland. Twenty patients had a benign mixed-cell tumour. They presented in a recognisable clinical manner with a painless mass in the region of the lacrimal gland which slowly enlarged over a period of at least 1 year before consultation. Twenty patients had a carcinoma of the lacrimal gland. They had a short history and experienced pain. On clinical grounds they could not be distinguished from inflammatory lesions in the region of the lacrimal gland. The method of treating these 2 groups of patients is described and methods of dealing logically with their problems are suggested.", "contents": "Clinical presentation and management of lacrimal gland tumours. This paper presents the clinical and pathological findings in 40 consecutive patients seen with primary tumours arising from the lacrimal gland. Twenty patients had a benign mixed-cell tumour. They presented in a recognisable clinical manner with a painless mass in the region of the lacrimal gland which slowly enlarged over a period of at least 1 year before consultation. Twenty patients had a carcinoma of the lacrimal gland. They had a short history and experienced pain. On clinical grounds they could not be distinguished from inflammatory lesions in the region of the lacrimal gland. The method of treating these 2 groups of patients is described and methods of dealing logically with their problems are suggested."}
{"id": "PMID:486379", "title": "Optic nerve injury in fracture of the canal.", "content": "A case of unilateral blindness following blunt injury to the skull is presented. The patient died 4 days after the initial injury, presenting the rare opportunity of a detailed histopathological study of the acute features of the condition. The findings are discussed in the context of current theories of the pathogenesis of optic nerve injury in fractures of the optic canal.", "contents": "Optic nerve injury in fracture of the canal. A case of unilateral blindness following blunt injury to the skull is presented. The patient died 4 days after the initial injury, presenting the rare opportunity of a detailed histopathological study of the acute features of the condition. The findings are discussed in the context of current theories of the pathogenesis of optic nerve injury in fractures of the optic canal."}
{"id": "PMID:486380", "title": "Phlyctenular eye disease in association with Hymenolepis nana in Egypt.", "content": "It had been previously noticed that infection with parasites was common in children suffering from phlyctenulosis. In the present study the stools of 471 patients suffering from phlyctenular disease were examined and it was found that 62.6% of them had Hymenolepis nana ova in their stools as compared with 10.8% of the controls. All patients had Hymenolipis nana immune sera. Many of these patients had abdominal symptoms. Hymenolepis nana is a cestode parasite discovered by Bilharz in Cairo in 1851. Infections with it have the same age incidence and geographical distribution as phlyctenular eye disease. It has a tissue stage responsible for a state of hypersensitivity which is thought to be responsible for the phlyctenules.", "contents": "Phlyctenular eye disease in association with Hymenolepis nana in Egypt. It had been previously noticed that infection with parasites was common in children suffering from phlyctenulosis. In the present study the stools of 471 patients suffering from phlyctenular disease were examined and it was found that 62.6% of them had Hymenolepis nana ova in their stools as compared with 10.8% of the controls. All patients had Hymenolipis nana immune sera. Many of these patients had abdominal symptoms. Hymenolepis nana is a cestode parasite discovered by Bilharz in Cairo in 1851. Infections with it have the same age incidence and geographical distribution as phlyctenular eye disease. It has a tissue stage responsible for a state of hypersensitivity which is thought to be responsible for the phlyctenules."}
{"id": "PMID:486381", "title": "Surgical management and histopathology of invasive tumours of the cornea.", "content": "Four cases of conjunctival squamous carcinoma invading the cornea are presented. The surgical technique of excising the tumours from the cornea by lamellar keratectomy is described. Histology of the lesions showed well differentiated squamous carcinoma invading the superficial layers of the cornea only. Large invasive squamous cell carcinomas of the cornea result because of neglect of small paralimbal tumours and can be adequately removed by lamellar keratectomy, leaving the globe intact.", "contents": "Surgical management and histopathology of invasive tumours of the cornea. Four cases of conjunctival squamous carcinoma invading the cornea are presented. The surgical technique of excising the tumours from the cornea by lamellar keratectomy is described. Histology of the lesions showed well differentiated squamous carcinoma invading the superficial layers of the cornea only. Large invasive squamous cell carcinomas of the cornea result because of neglect of small paralimbal tumours and can be adequately removed by lamellar keratectomy, leaving the globe intact."}
{"id": "PMID:486382", "title": "Trabeculectomy in Nigerian patients with open-angle glaucoma.", "content": "Filtering procedures for glaucoma in Africans are considered to be failures owing to their propensity for increased fibrosis. However, favourable results from trabeculectomy in African patients with glaucoma are reported. Most of these are from such modifications of technique as non-suturing of flaps, sclerectomy, posterior lip cautery, or administration of high doses of steroids. In this study 95.4% success with a virtual absence of complications is reported from a standard technique of trabeculectomy and no steroids.", "contents": "Trabeculectomy in Nigerian patients with open-angle glaucoma. Filtering procedures for glaucoma in Africans are considered to be failures owing to their propensity for increased fibrosis. However, favourable results from trabeculectomy in African patients with glaucoma are reported. Most of these are from such modifications of technique as non-suturing of flaps, sclerectomy, posterior lip cautery, or administration of high doses of steroids. In this study 95.4% success with a virtual absence of complications is reported from a standard technique of trabeculectomy and no steroids."}
{"id": "PMID:486383", "title": "Trabeculectomy versus Scheie's operation: a comparative retrospective study in open-angle glaucoma in Kenyans.", "content": "A comparative retrospective study was performed on 39 patients with open-angle glaucoma in whom the right eye was subjected to Scheie's operation, the left to trabeculectomy. Trabeculectomy without the use of additional therapy or reoperation brought the intraocular pressure to under 22 mmHg in 80% of the cases compared with 74% treated by Scheie's operation. Reduction of visual acuity postoperatively was 4 times more frequent with Scheie's operation than with trabeculectomy. A report on a second group of patients in whom 47 trabeculectomies were performed is also given.", "contents": "Trabeculectomy versus Scheie's operation: a comparative retrospective study in open-angle glaucoma in Kenyans. A comparative retrospective study was performed on 39 patients with open-angle glaucoma in whom the right eye was subjected to Scheie's operation, the left to trabeculectomy. Trabeculectomy without the use of additional therapy or reoperation brought the intraocular pressure to under 22 mmHg in 80% of the cases compared with 74% treated by Scheie's operation. Reduction of visual acuity postoperatively was 4 times more frequent with Scheie's operation than with trabeculectomy. A report on a second group of patients in whom 47 trabeculectomies were performed is also given."}
{"id": "PMID:486384", "title": "Assessment of the heart-rate method for determining energy expenditure in man, using a whole-body calorimeter.", "content": "1. The heart-rate (HR) method for determining the energy expenditure of free-living subjects has been evaluated using a whole-body calorimeter in which individuals lived continuously for 27 h while carrying out normal daily activities. Eight male volunteers each occupied the calorimeter on at least two occasions when HR and energy expenditure were measured continuously. 2. After each session in the calorimeter a calibration was obtained using standard techniques by determining HR and heat production (HP) over periods of 10-15 min at several levels of activity. Energy expenditure in the calorimeter was then predicted, by each of five methods, from the mean HR in the calorimeter. Additionally, one session in the calorimeter was used to obtain a calibration and was used for predicting the subject's energy expenditure while in the calorimeter on other occasions. 3. Standard methods of prediction using one calibration point at rest and several points during activity were unreliable for predicting the energy expenditure of an individual. The 24 h HR was at the lower end of the calibration scale and there were considerable over-estimates or underestimates of energy expenditure, particularly during the night when the mean (+/- SD) difference between the actual and predicted HP was -66 +/- 38.6%. A linear regression fitted to points at the lower levels of activity improved the prediction of 24 h HP while a logistic plot reduced the error even further. The best estimate of energy expenditure was that obtained from a calibration over 24 h within the calorimeter; the mean (+/- SD) difference between the actual and predicted 24 h HP was +3 +/- 10.5% for light activity and -3 +/- 6.7% for moderate activity. Thus current procedures for calibrating subjects may lead to large errors which could be reduced by using a respiratory chamber.", "contents": "Assessment of the heart-rate method for determining energy expenditure in man, using a whole-body calorimeter. 1. The heart-rate (HR) method for determining the energy expenditure of free-living subjects has been evaluated using a whole-body calorimeter in which individuals lived continuously for 27 h while carrying out normal daily activities. Eight male volunteers each occupied the calorimeter on at least two occasions when HR and energy expenditure were measured continuously. 2. After each session in the calorimeter a calibration was obtained using standard techniques by determining HR and heat production (HP) over periods of 10-15 min at several levels of activity. Energy expenditure in the calorimeter was then predicted, by each of five methods, from the mean HR in the calorimeter. Additionally, one session in the calorimeter was used to obtain a calibration and was used for predicting the subject's energy expenditure while in the calorimeter on other occasions. 3. Standard methods of prediction using one calibration point at rest and several points during activity were unreliable for predicting the energy expenditure of an individual. The 24 h HR was at the lower end of the calibration scale and there were considerable over-estimates or underestimates of energy expenditure, particularly during the night when the mean (+/- SD) difference between the actual and predicted HP was -66 +/- 38.6%. A linear regression fitted to points at the lower levels of activity improved the prediction of 24 h HP while a logistic plot reduced the error even further. The best estimate of energy expenditure was that obtained from a calibration over 24 h within the calorimeter; the mean (+/- SD) difference between the actual and predicted 24 h HP was +3 +/- 10.5% for light activity and -3 +/- 6.7% for moderate activity. Thus current procedures for calibrating subjects may lead to large errors which could be reduced by using a respiratory chamber."}
{"id": "PMID:486385", "title": "The differential effect of magnesium deficiency on isoenzymes of alkaline phosphatase (EC 3.1.3.1) in rat tissues.", "content": "1. Alkaline phosphatase (EC 3.1.3.1) from many rat tissues was separated into two or three bands by electrophoresis on polyacrylamide gel. Ten of the bands had different electrophoretic mobilities, but some were present in more than one tissue. 2. Bands from the corresponding tissues of magnesium-deficient and control rats were qualitatively similar, but there were quantitative differences in the distribution of enzyme activity between them. 3. Mg deficiency had differential effects on the absolute activity of individual bands, but two main types of response were observed. There was an increase in the activity of the first bands from liver and kidney, the second band from femur and both bands from spleen, whereas the first band from femur, the first and second bands from intestinal mucosa and the second bands of serum, liver and kidney all decreased in activity during the deficiency. 4. The change in total alkaline phosphatase activity of a tissue during Mg deficiency depended on the ratio between the enzyme components within it.", "contents": "The differential effect of magnesium deficiency on isoenzymes of alkaline phosphatase (EC 3.1.3.1) in rat tissues. 1. Alkaline phosphatase (EC 3.1.3.1) from many rat tissues was separated into two or three bands by electrophoresis on polyacrylamide gel. Ten of the bands had different electrophoretic mobilities, but some were present in more than one tissue. 2. Bands from the corresponding tissues of magnesium-deficient and control rats were qualitatively similar, but there were quantitative differences in the distribution of enzyme activity between them. 3. Mg deficiency had differential effects on the absolute activity of individual bands, but two main types of response were observed. There was an increase in the activity of the first bands from liver and kidney, the second band from femur and both bands from spleen, whereas the first band from femur, the first and second bands from intestinal mucosa and the second bands of serum, liver and kidney all decreased in activity during the deficiency. 4. The change in total alkaline phosphatase activity of a tissue during Mg deficiency depended on the ratio between the enzyme components within it."}
{"id": "PMID:486386", "title": "A comparative study of the absorption of calcium and the availability of phytate-phosphorus in the golden hamster (Mesocricetus auratus) and the laboratory rat.", "content": "1. Balance studies were carried out with rats and golden hamsters (Mesocricetus auratus) given diets high and low in calcium (10.8 and 5.1-5.2 g Ca/kg respectively) and high and low in phytate (3.4 and 1.6 g phytate-phosphorus/kg respectively) in a 2 X 2 factorial experiment. 2. Values for Ca absorption and phytate availability (relative to the amount ingested) and for urinary excretion of Ca and P were significantly higher in the hamster than in the rat for all four diets. 3. Phytate  availability was significantly greater on the low-Ca than on the high-Ca diets with both species. 4. Ca absorption was greater on the low-phytate than on the high-phytate diets but the differences were significant only for the hamster. 5. Absorption of both Ca and P was poorly controlled in the hamster compared to the rat.", "contents": "A comparative study of the absorption of calcium and the availability of phytate-phosphorus in the golden hamster (Mesocricetus auratus) and the laboratory rat. 1. Balance studies were carried out with rats and golden hamsters (Mesocricetus auratus) given diets high and low in calcium (10.8 and 5.1-5.2 g Ca/kg respectively) and high and low in phytate (3.4 and 1.6 g phytate-phosphorus/kg respectively) in a 2 X 2 factorial experiment. 2. Values for Ca absorption and phytate availability (relative to the amount ingested) and for urinary excretion of Ca and P were significantly higher in the hamster than in the rat for all four diets. 3. Phytate  availability was significantly greater on the low-Ca than on the high-Ca diets with both species. 4. Ca absorption was greater on the low-phytate than on the high-phytate diets but the differences were significant only for the hamster. 5. Absorption of both Ca and P was poorly controlled in the hamster compared to the rat."}
{"id": "PMID:486387", "title": "Kinetics of native and modified Bowman-Birk soya-bean trypsin inhibitor on growth and enzymes activities of the chick pancreas.", "content": "1. The Bowman-Birk soya-bean trypsin inhibitor (BBTI) begins to cause pancreatic enlargement and increased enzymic activity in the pancreas of chicks after a minimum of 7 d of feeding. 2. The active inhibitory site of BBTI against trypsin is the factor involved in the pancreatic enlargement and increase of pancreatic enzyme activity in chicks.", "contents": "Kinetics of native and modified Bowman-Birk soya-bean trypsin inhibitor on growth and enzymes activities of the chick pancreas. 1. The Bowman-Birk soya-bean trypsin inhibitor (BBTI) begins to cause pancreatic enlargement and increased enzymic activity in the pancreas of chicks after a minimum of 7 d of feeding. 2. The active inhibitory site of BBTI against trypsin is the factor involved in the pancreatic enlargement and increase of pancreatic enzyme activity in chicks."}
{"id": "PMID:486390", "title": "The effect of a low-protein ration on milk yield and plasma metabolites in Friesian heifers during early lactation.", "content": "1. Sixteen first-calf Friesian heifers were used in a continous treatment design experiment. For 2 weeks after calving they were given a 750 g concentrate, 250 g hay/kg ration with 169 g crude protein (nitrogen X 6.25; CP)/kg dry matter (DM). They were then divided into two groups of eight and given a high-protein (223 g CP/kg DM) or low-protein (107 g CP/kg DM) ration at the rate of 10.8 kg concentrate + 3.6 kg hay for 8 weeks. 2. Milk yield and composition, live weight and blood composition were monitored throughout. A digestibility trial was carried out with six animals on each treatment. 3. The low protein ration reduced DM, organic matter, energy and fibre digestibility significantly (P less than 0.001) so that intakes of digestible energy were not equal and the low-protein group lost more weight than the high-protein group. 4. Milk yield and the fat content of milk were lower in heifers given the low-protein ration (P less than 0.01). The lactose content of the milk was not affected and protein content only slightly reduced (P less than 0.01) by low-protein feeding. When the heifers were all changed onto an adequate protein (190 g CP/kg DM) ration in mid-lactation, those which had previously been under-fed protein appeared to recover in milk yield to the point they might have been expected to reach is given an adequate-protein ration throughout. 5. Concentrations of urea (P less than 0.001) and albumin (P less than 0.05) were reduced by underfeeding protein, but albumin concentration was affected less by diet than by stage of lactation. Blood concentrations of total protein, glucose, sodium, potassium, calcium, inorganic phosphate, iron, copper, haemoglobin and packed cell volume were unaffected by treatment. Blood magnesium concentration was slightly lower (P less than 0.01) with low-protein feeding.", "contents": "The effect of a low-protein ration on milk yield and plasma metabolites in Friesian heifers during early lactation. 1. Sixteen first-calf Friesian heifers were used in a continous treatment design experiment. For 2 weeks after calving they were given a 750 g concentrate, 250 g hay/kg ration with 169 g crude protein (nitrogen X 6.25; CP)/kg dry matter (DM). They were then divided into two groups of eight and given a high-protein (223 g CP/kg DM) or low-protein (107 g CP/kg DM) ration at the rate of 10.8 kg concentrate + 3.6 kg hay for 8 weeks. 2. Milk yield and composition, live weight and blood composition were monitored throughout. A digestibility trial was carried out with six animals on each treatment. 3. The low protein ration reduced DM, organic matter, energy and fibre digestibility significantly (P less than 0.001) so that intakes of digestible energy were not equal and the low-protein group lost more weight than the high-protein group. 4. Milk yield and the fat content of milk were lower in heifers given the low-protein ration (P less than 0.01). The lactose content of the milk was not affected and protein content only slightly reduced (P less than 0.01) by low-protein feeding. When the heifers were all changed onto an adequate protein (190 g CP/kg DM) ration in mid-lactation, those which had previously been under-fed protein appeared to recover in milk yield to the point they might have been expected to reach is given an adequate-protein ration throughout. 5. Concentrations of urea (P less than 0.001) and albumin (P less than 0.05) were reduced by underfeeding protein, but albumin concentration was affected less by diet than by stage of lactation. Blood concentrations of total protein, glucose, sodium, potassium, calcium, inorganic phosphate, iron, copper, haemoglobin and packed cell volume were unaffected by treatment. Blood magnesium concentration was slightly lower (P less than 0.01) with low-protein feeding."}
{"id": "PMID:486391", "title": "Absorption of vitamin C from the human buccal cavity.", "content": "1. Ascorbic acid was absorbed across the mucosa of the human mouth. 2. Omission of sodium ions from the medium decreased the  absorption of ascorbic acid. 3. The presence of D-glucose, or 3-O-methyl-D-glucose, increased the absorption of ascorbic acid but D-fructose had little effect and D-mannitol had no effect. 4. Calcium ions also increased ascorbic acid absorption probably by a secondary effect on \"Na+ fluxes. 5. Buccal mucosa was also permeable to dehydroascorbic acid and D-isoascorbic acid.", "contents": "Absorption of vitamin C from the human buccal cavity. 1. Ascorbic acid was absorbed across the mucosa of the human mouth. 2. Omission of sodium ions from the medium decreased the  absorption of ascorbic acid. 3. The presence of D-glucose, or 3-O-methyl-D-glucose, increased the absorption of ascorbic acid but D-fructose had little effect and D-mannitol had no effect. 4. Calcium ions also increased ascorbic acid absorption probably by a secondary effect on \"Na+ fluxes. 5. Buccal mucosa was also permeable to dehydroascorbic acid and D-isoascorbic acid."}
{"id": "PMID:486392", "title": "Long-term vitamin status and dietary intake of healthy elderly subjects. 1. Riboflavin.", "content": "1. Long-term clinical and biochemical riboflavin status and dietary intake of riboflavin were monitored for 18 months in a group of twenty-three relatively-healthy elderly subjects living at home in the north of England. 2. Both dietary intake and biochemical status, as measured by the activation coefficient (stimulated:basal activity) of NAD(P)H2:glutathione oxidoreductase (EC 1.6.4.2), remained fairly constant for each individual and for most subjects the usual intake and biochemical status were characterized quite accurately by a single week's intake dietary record and a single measurement of the activation coefficient. 3. The expected relationship between biochemical status and dietary intake was present, but not strongly evident. There was a significant within-subject correlation between alpha values and the immediately preceding dietary intake; however the between-subject correlation between alpha values and dietary intake approached zero if the two subjects receiving long-term riboflavin supplements were omitted. Over the limited range of intakes observed in unsupplemented subjects, non-dietary factors evidently obscure the relationship. 4. Although eight subjects had average values above 1.2 for the activation coefficient, no excessively high values were observed, and no subject showed any clear-cut clinical deficiency symptoms.", "contents": "Long-term vitamin status and dietary intake of healthy elderly subjects. 1. Riboflavin. 1. Long-term clinical and biochemical riboflavin status and dietary intake of riboflavin were monitored for 18 months in a group of twenty-three relatively-healthy elderly subjects living at home in the north of England. 2. Both dietary intake and biochemical status, as measured by the activation coefficient (stimulated:basal activity) of NAD(P)H2:glutathione oxidoreductase (EC 1.6.4.2), remained fairly constant for each individual and for most subjects the usual intake and biochemical status were characterized quite accurately by a single week's intake dietary record and a single measurement of the activation coefficient. 3. The expected relationship between biochemical status and dietary intake was present, but not strongly evident. There was a significant within-subject correlation between alpha values and the immediately preceding dietary intake; however the between-subject correlation between alpha values and dietary intake approached zero if the two subjects receiving long-term riboflavin supplements were omitted. Over the limited range of intakes observed in unsupplemented subjects, non-dietary factors evidently obscure the relationship. 4. Although eight subjects had average values above 1.2 for the activation coefficient, no excessively high values were observed, and no subject showed any clear-cut clinical deficiency symptoms."}
{"id": "PMID:486394", "title": "Site differences in the fatty acid composition of subcutaneous adipose tissue of obese women.", "content": "1. Adipose tissue samples were obtained by needle biopsy from three subcutaneous sites (thigh, abdomen and upper arm) in twenty-two obese women. The fatty acid composition was determined using gas-liquid chromatography and the results presented relate to eleven component fatty acids. 2. The fatty acid composition of adipose tissue obtained from the arm and abdomen was remarkably similar, with the exception of the levels of lauric acid. 3. The analyses showed that the majority of the saturated fatty acids were present in smaller proportions whilst the majority of unsaturated fatty acids were present in larger proportions in the thigh than in the two other sites. Highly significant inter-site differences were demonstrated for six of the major fatty acids and also for both the total amounts of saturated and unsaturated fatty acids and their ratios. 4. No marked differences in the fatty acid composition of adipose tissue from obese subjects were revealed during this study when compared with previously reported results obtained from 'normal-weight' subjects.", "contents": "Site differences in the fatty acid composition of subcutaneous adipose tissue of obese women. 1. Adipose tissue samples were obtained by needle biopsy from three subcutaneous sites (thigh, abdomen and upper arm) in twenty-two obese women. The fatty acid composition was determined using gas-liquid chromatography and the results presented relate to eleven component fatty acids. 2. The fatty acid composition of adipose tissue obtained from the arm and abdomen was remarkably similar, with the exception of the levels of lauric acid. 3. The analyses showed that the majority of the saturated fatty acids were present in smaller proportions whilst the majority of unsaturated fatty acids were present in larger proportions in the thigh than in the two other sites. Highly significant inter-site differences were demonstrated for six of the major fatty acids and also for both the total amounts of saturated and unsaturated fatty acids and their ratios. 4. No marked differences in the fatty acid composition of adipose tissue from obese subjects were revealed during this study when compared with previously reported results obtained from 'normal-weight' subjects."}
{"id": "PMID:486396", "title": "The effects of defaunation of the rumen on the growth of lambs on low-protein-high-energy diets.", "content": "1. The effects of defaunation of the rumen of lambs given low-protein-high-energy diets were studied using animals fed ad lib. a basal diet of sugar and oaten chaff which was supplemented with fish meal at various levels. These diets supported relatively large populations of protozoa in the rumen. 2. A nonyl phenol ethoxylate (15 g. Teric GN9:ICI Australian Ltd) given intraruminally, was shown to be an effective means of defaunating the rumen and the lambs were maintained protozoa-free by isolation. 3. In lambs given low levels of fish meal in the diet, the absence of rumen protozoa increased growth rate and improved the food conversion efficiency (g dry matter intake/g growth). These effects were not apparent at high levels of protein supplementation. 4. These results suggested that the absence of rumen protozoa resulted in an increased availability of nutrients supplying both energy and amino acids since the efficiency of food utilization and the rate of wool growth (which is sensitive to amino acid supply) were increased in lambs on the low level of protein supplementation without apparently increasing food intake.", "contents": "The effects of defaunation of the rumen on the growth of lambs on low-protein-high-energy diets. 1. The effects of defaunation of the rumen of lambs given low-protein-high-energy diets were studied using animals fed ad lib. a basal diet of sugar and oaten chaff which was supplemented with fish meal at various levels. These diets supported relatively large populations of protozoa in the rumen. 2. A nonyl phenol ethoxylate (15 g. Teric GN9:ICI Australian Ltd) given intraruminally, was shown to be an effective means of defaunating the rumen and the lambs were maintained protozoa-free by isolation. 3. In lambs given low levels of fish meal in the diet, the absence of rumen protozoa increased growth rate and improved the food conversion efficiency (g dry matter intake/g growth). These effects were not apparent at high levels of protein supplementation. 4. These results suggested that the absence of rumen protozoa resulted in an increased availability of nutrients supplying both energy and amino acids since the efficiency of food utilization and the rate of wool growth (which is sensitive to amino acid supply) were increased in lambs on the low level of protein supplementation without apparently increasing food intake."}
{"id": "PMID:486398", "title": "The effect of intestinal coccidiosis (Eimeria acervulina) on blood and tissue ascorbic acid concentrations.", "content": "1. Two experiments were conducted to investigate the effect of intestinal coccidiosis (Eimeria acervulina) on blood plasma and tissue ascorbic acid (AA) concentrations in chicks, and a third experiment was carried out to examine the role of dietary AA in this infection. 2. Experimental infection with intestinal coccidiosis (Eimeria acervulina) brought about a depletion in the concentration of AA in blood plasma, duodenum, jejunum, ileum, liver and the adrenal glands. Generally, these changes were obtained after the prepatent period of 4-5 d of the infection. 3. Dietary AA at 1000 mg/kg prevented the depletion in plasma and tissue AA levels but did not significantly affect adrenal weight.", "contents": "The effect of intestinal coccidiosis (Eimeria acervulina) on blood and tissue ascorbic acid concentrations. 1. Two experiments were conducted to investigate the effect of intestinal coccidiosis (Eimeria acervulina) on blood plasma and tissue ascorbic acid (AA) concentrations in chicks, and a third experiment was carried out to examine the role of dietary AA in this infection. 2. Experimental infection with intestinal coccidiosis (Eimeria acervulina) brought about a depletion in the concentration of AA in blood plasma, duodenum, jejunum, ileum, liver and the adrenal glands. Generally, these changes were obtained after the prepatent period of 4-5 d of the infection. 3. Dietary AA at 1000 mg/kg prevented the depletion in plasma and tissue AA levels but did not significantly affect adrenal weight."}
{"id": "PMID:486399", "title": "Complete assignment of carbon signals in a stereospecific peptide via selective and single off-resonance proton decoupling experiments. Analysis of the carbon-13 nuclear magnetic resonance spectrum of alumichrome at 67.88 MHz.", "content": "Polypeptides and proteins in native conformation exhibit 13C NMR spectra which are highly nondegenerate. Assignment of resonances to carbons in particular residues is hence a prerequisite for a structural analysis of the spectroscopic data. For nonprotonated carbonyl carbons, the assignment can be achieved by selective (1H alpha)13C'  2J decoupling. Using this method, we have assigned the Orn1 and Gly2 carbonyl resonances in alumichrome at 67.9 MHz. We show that a single off-resonance experiment with the decoupling frequency centered in the aliphatic proton spectrum is sufficient to assign unequivocally all the protonated carbon resonances via analysis of the reduced 1J heteronuclear splittings. Alumichrome thus becomes the first complex polypeptide spin system whose 1H, 15N, and now 13C nuclear resonances have been fully identified to date. 13C chemical shifts and 1H--13C spin--spin couplings are discussed in terms of structural strain leading to specific orbital hybridizations and on the basis of polarization effects due to electron density shifts toward hydrogen-bonding and metal-binding sites. A number of 3J(13C--C--C--1H) coupling constants measured on selected multiplets after resolution enhancement were used to derive the x-related Karplus relationship 3J(theta) = (10.2 cos2 theta -- 1.3 cos theta + 0.2) Hz.", "contents": "Complete assignment of carbon signals in a stereospecific peptide via selective and single off-resonance proton decoupling experiments. Analysis of the carbon-13 nuclear magnetic resonance spectrum of alumichrome at 67.88 MHz. Polypeptides and proteins in native conformation exhibit 13C NMR spectra which are highly nondegenerate. Assignment of resonances to carbons in particular residues is hence a prerequisite for a structural analysis of the spectroscopic data. For nonprotonated carbonyl carbons, the assignment can be achieved by selective (1H alpha)13C'  2J decoupling. Using this method, we have assigned the Orn1 and Gly2 carbonyl resonances in alumichrome at 67.9 MHz. We show that a single off-resonance experiment with the decoupling frequency centered in the aliphatic proton spectrum is sufficient to assign unequivocally all the protonated carbon resonances via analysis of the reduced 1J heteronuclear splittings. Alumichrome thus becomes the first complex polypeptide spin system whose 1H, 15N, and now 13C nuclear resonances have been fully identified to date. 13C chemical shifts and 1H--13C spin--spin couplings are discussed in terms of structural strain leading to specific orbital hybridizations and on the basis of polarization effects due to electron density shifts toward hydrogen-bonding and metal-binding sites. A number of 3J(13C--C--C--1H) coupling constants measured on selected multiplets after resolution enhancement were used to derive the x-related Karplus relationship 3J(theta) = (10.2 cos2 theta -- 1.3 cos theta + 0.2) Hz."}
{"id": "PMID:486400", "title": "Partial characterization of a tropoelastin precursor isolated from chick aorta.", "content": "Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e., a proelastin, it is proposed to be at least a truncated form of proelastin that is converted to tropoelastin. The key to its isolation was the presence of alpha 1-antitrypsin at each step in the purification procedure. The first 11 amino acid residues at the NH2 terminal of the proposed tropoelastin precursor (GGVPGVAVPGGV) are the same as those for tropoelastin. Its amino acid composition is similar to that of tropoelastin, except for higher amounts of acidic amino acid residues. Further, the proposed precursor contains a limited number of aldehydic functions, presumably in the form of peptidyl allysine. This was taken as an indication that the proposed precursor serves as a substract for lysyl oxidase. Under the conditions used for the isolation, the precursor appeared to be in higher concentrations than tropoelastin in aorta extracts from copper-deficient chicks.", "contents": "Partial characterization of a tropoelastin precursor isolated from chick aorta. Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e., a proelastin, it is proposed to be at least a truncated form of proelastin that is converted to tropoelastin. The key to its isolation was the presence of alpha 1-antitrypsin at each step in the purification procedure. The first 11 amino acid residues at the NH2 terminal of the proposed tropoelastin precursor (GGVPGVAVPGGV) are the same as those for tropoelastin. Its amino acid composition is similar to that of tropoelastin, except for higher amounts of acidic amino acid residues. Further, the proposed precursor contains a limited number of aldehydic functions, presumably in the form of peptidyl allysine. This was taken as an indication that the proposed precursor serves as a substract for lysyl oxidase. Under the conditions used for the isolation, the precursor appeared to be in higher concentrations than tropoelastin in aorta extracts from copper-deficient chicks."}
{"id": "PMID:486401", "title": "Determination of free and bound microtubular protein and guanine nucleotide under equilibrium conditions.", "content": "The dissociation constant for GDP binding to the E site of tubulin isolated by chromatography on Sepharose 6B is equal to 6.1 X 10(-8) M, as determined by the Hummel-Dryer procedure. This is smaller than any previously reported value, and the discrepancy with earlir results is analyzed. By use of a recently described column centrifugation procedure [Penefsky, H. S. (1977) J. Biol. Chem. 252, 2891-2899], it was established that GDP and GTP bind to the same site. GTP is bound 2.8-fold tighter than GDP, and the dissociation constant is 2.2 X 10(-8) M. A new method for the determination of dissociation constants for a protein-bound ligand, based on a quantitative analysis of the loss of ligand during exclusion chromatography, is presented. This has been used to determine that the dissociation constant for GDP bound to tubulin is equal to 5.5 X 10(-8) M, in excellent agreement with that determined independently from the Hummel-Dryer method. A previous theoretical treatment [Dixon, H. B. F. (1976) Biochem. J. 159, 161-162] of ligand loss during exclusion chromatography is discussed.", "contents": "Determination of free and bound microtubular protein and guanine nucleotide under equilibrium conditions. The dissociation constant for GDP binding to the E site of tubulin isolated by chromatography on Sepharose 6B is equal to 6.1 X 10(-8) M, as determined by the Hummel-Dryer procedure. This is smaller than any previously reported value, and the discrepancy with earlir results is analyzed. By use of a recently described column centrifugation procedure [Penefsky, H. S. (1977) J. Biol. Chem. 252, 2891-2899], it was established that GDP and GTP bind to the same site. GTP is bound 2.8-fold tighter than GDP, and the dissociation constant is 2.2 X 10(-8) M. A new method for the determination of dissociation constants for a protein-bound ligand, based on a quantitative analysis of the loss of ligand during exclusion chromatography, is presented. This has been used to determine that the dissociation constant for GDP bound to tubulin is equal to 5.5 X 10(-8) M, in excellent agreement with that determined independently from the Hummel-Dryer method. A previous theoretical treatment [Dixon, H. B. F. (1976) Biochem. J. 159, 161-162] of ligand loss during exclusion chromatography is discussed."}
{"id": "PMID:486402", "title": "Studies with mechanism-based inactivators of lysine epsilon-transaminase from Achromobacter liquidum.", "content": "Analogues of lysine containing a 4,5-acetylenic linkage (lysyne) or a cis- or trans-4,5-olefinic linkage (lysenes) function as substrates for a homogeneous L-lysine epsilon-transaminase from Achromobacter liquidum but partition between transamination and time-dependent inactivation. The partition ratio is lowest for lysyne (40 per inactivation event) and higher for trans-lysene (160 per inactivation event), and the cis-lysene transaminates 1600 times per inactivation event. cis-Lysene yields alpha-picolinate as a detectable accumulating product, presumably from cyclization of initial 6-aldehyde to dihydropicolinate and spontaneous autoxidation. The trans isomer also yields some picolinate as an identifiable product. The product from the few lysyne turnovers is as yet unknown but has strong absorbance at 318 nm. The inactive enzyme species from all three lysine analogues slowly (overnight) regain full activity after gel filtration chromatography and dialysis, suggesting reversal of the initial adduct-forming reaction. Initial studies with partially purified pseudomonad lysine alpha-racemase show alpha-3H incorporation from 3H2O but no inactivation consistent with the expectation that these lysine analogues could act readily as mechanism-based inactivators for pyridoxal P enzymes which act at the epsilon- but not the alpha-carbon of lysine.", "contents": "Studies with mechanism-based inactivators of lysine epsilon-transaminase from Achromobacter liquidum. Analogues of lysine containing a 4,5-acetylenic linkage (lysyne) or a cis- or trans-4,5-olefinic linkage (lysenes) function as substrates for a homogeneous L-lysine epsilon-transaminase from Achromobacter liquidum but partition between transamination and time-dependent inactivation. The partition ratio is lowest for lysyne (40 per inactivation event) and higher for trans-lysene (160 per inactivation event), and the cis-lysene transaminates 1600 times per inactivation event. cis-Lysene yields alpha-picolinate as a detectable accumulating product, presumably from cyclization of initial 6-aldehyde to dihydropicolinate and spontaneous autoxidation. The trans isomer also yields some picolinate as an identifiable product. The product from the few lysyne turnovers is as yet unknown but has strong absorbance at 318 nm. The inactive enzyme species from all three lysine analogues slowly (overnight) regain full activity after gel filtration chromatography and dialysis, suggesting reversal of the initial adduct-forming reaction. Initial studies with partially purified pseudomonad lysine alpha-racemase show alpha-3H incorporation from 3H2O but no inactivation consistent with the expectation that these lysine analogues could act readily as mechanism-based inactivators for pyridoxal P enzymes which act at the epsilon- but not the alpha-carbon of lysine."}
{"id": "PMID:486404", "title": "Isolation and characterization of the histone variants in chicken erythrocytes.", "content": "Chicken erythrocyte histones 2A, 2B, and 3 can be resolved into nonallelic primary structure variants by polyacrylamide gel electrophoresis in the presence of Triton X-100. These variants were isolated and characterized by analysis of their tryptic and thermolytic peptides. The major variants of chicken H2A and H2B differ from the analogous component of calf thymus by a small number of conservative amino acid substitutions in the basic terminal regions, which interact with DNA. This moderate rate of allelic evolution of the slightly lysine-rich histones contrasts with the complete conservatism found in the arginine-rich histones. Chicken H4 and both chicken H3 variants are identical with their corresponding components in mammals. The amino acid substitutions distinguishing histone variants are located within the highly conserved hydrophobic regions, which are involved in histone--histone interactions.", "contents": "Isolation and characterization of the histone variants in chicken erythrocytes. Chicken erythrocyte histones 2A, 2B, and 3 can be resolved into nonallelic primary structure variants by polyacrylamide gel electrophoresis in the presence of Triton X-100. These variants were isolated and characterized by analysis of their tryptic and thermolytic peptides. The major variants of chicken H2A and H2B differ from the analogous component of calf thymus by a small number of conservative amino acid substitutions in the basic terminal regions, which interact with DNA. This moderate rate of allelic evolution of the slightly lysine-rich histones contrasts with the complete conservatism found in the arginine-rich histones. Chicken H4 and both chicken H3 variants are identical with their corresponding components in mammals. The amino acid substitutions distinguishing histone variants are located within the highly conserved hydrophobic regions, which are involved in histone--histone interactions."}
{"id": "PMID:486406", "title": "Deoxyribonucleic acid excision repair in chromatin after ultraviolet irradiation of human fibroblasts in culture.", "content": "We have exposed confluent normal human fibroblasts to ultraviolet (UV) fluences of 5, 14, or 40 J/m2 and monitored the specific activity of post-UV repair synthesis in chromatin with [3H]thymidine pulses. We have shown that under conditions where no semiconservative deoxyribonucleic acid (DNA) synthesis is detectable, the specific activity of repair label in micrococcal nuclease resistant (core particle) DNA is about one-fifth that in bulk DNA at all three UV fluences. On the other hand, the distribution of thymine-containing pyrimidine dimers in bulk and nuclease-resistant regions measured either immediately after irradiation or at later times showed no significant differences; preferential labeling of linker (nuclease-sensitive) DNA during repair synthesis is thus apparently not due to a predominance of UV-induced photoproducts in linker relative to core particle DNA in the nucleosome. Pulse and pulse--chase experiments at 14 or 40 J/m2 with normal human or repair-deficient xeroderma pigmentosum (XP) cells showed that at most 30% of repair label in all these cells shifts from nuclease-sensitive (linker) DNA to nuclease-resistant (core particle) DNA.", "contents": "Deoxyribonucleic acid excision repair in chromatin after ultraviolet irradiation of human fibroblasts in culture. We have exposed confluent normal human fibroblasts to ultraviolet (UV) fluences of 5, 14, or 40 J/m2 and monitored the specific activity of post-UV repair synthesis in chromatin with [3H]thymidine pulses. We have shown that under conditions where no semiconservative deoxyribonucleic acid (DNA) synthesis is detectable, the specific activity of repair label in micrococcal nuclease resistant (core particle) DNA is about one-fifth that in bulk DNA at all three UV fluences. On the other hand, the distribution of thymine-containing pyrimidine dimers in bulk and nuclease-resistant regions measured either immediately after irradiation or at later times showed no significant differences; preferential labeling of linker (nuclease-sensitive) DNA during repair synthesis is thus apparently not due to a predominance of UV-induced photoproducts in linker relative to core particle DNA in the nucleosome. Pulse and pulse--chase experiments at 14 or 40 J/m2 with normal human or repair-deficient xeroderma pigmentosum (XP) cells showed that at most 30% of repair label in all these cells shifts from nuclease-sensitive (linker) DNA to nuclease-resistant (core particle) DNA."}
{"id": "PMID:486407", "title": "Carbon-13 nuclear magnetic resonance studies on tobacco mosaic virus and its protein.", "content": "Fourier transform nuclear magnetic resonance studies on 12% 13C-enriched tobacco mosaic virus (TMV) and its rod-like protein oligomers in solution with molecular weights up to 42 X 10(6) are reported. In the virus approximately 17% of the carbons of the protein subunit have line widths of less than or equal to 300 Hz and T1 less than or equal to 1 s and are concluded to be mobile with more than one degree of freedom of internal rotation about a carbon--carbon bond. In the rodlike polymer of TMV protein at pH 5.3, 30% of the carbons are mobile, which implies rotational motions about carbon--carbon bonds and/or motions of the protein subunits within the polymer. The presence of internal mobility is supported by the observation that 20% of the carbons in the double disklike oligomer show decreasing line width upon increasing temperature; the remaining resonances have line widths which are temperature independent during the double disklike polymerization process. Since the molecular weight of TMV protein polymers increases with increasing temperature, this demonstrates that all nuclei within the double dislike oligomer are mobile. NMR and X-ray data on the double disklike polymer reveal differences with respect to internal mobility.", "contents": "Carbon-13 nuclear magnetic resonance studies on tobacco mosaic virus and its protein. Fourier transform nuclear magnetic resonance studies on 12% 13C-enriched tobacco mosaic virus (TMV) and its rod-like protein oligomers in solution with molecular weights up to 42 X 10(6) are reported. In the virus approximately 17% of the carbons of the protein subunit have line widths of less than or equal to 300 Hz and T1 less than or equal to 1 s and are concluded to be mobile with more than one degree of freedom of internal rotation about a carbon--carbon bond. In the rodlike polymer of TMV protein at pH 5.3, 30% of the carbons are mobile, which implies rotational motions about carbon--carbon bonds and/or motions of the protein subunits within the polymer. The presence of internal mobility is supported by the observation that 20% of the carbons in the double disklike oligomer show decreasing line width upon increasing temperature; the remaining resonances have line widths which are temperature independent during the double disklike polymerization process. Since the molecular weight of TMV protein polymers increases with increasing temperature, this demonstrates that all nuclei within the double dislike oligomer are mobile. NMR and X-ray data on the double disklike polymer reveal differences with respect to internal mobility."}
{"id": "PMID:486408", "title": "Isolation and characterization of 1 alpha-hydroxy-23-carboxytetranorvitamin D: a major metabolite of 1,25-dihydroxyvitamin D3.", "content": "The in vivo side-chain oxidation of 1 alpha,25-dihydroxyvitamin D3 was investigated by using a double-label radiotracer technique. Rats dosed with 1 alpha,25-dihydroxy-[3 alpha-3H]vitamin D3 and 1 alpha,25-dihydroxy[26,27-14C]vitamin D3 produced compounds with a high 3H/14C ratio. These compounds were found in sizable quantities in intestine and liver within 3 h after dosing. The major side-chain oxidized metabolite migrated as an acid on DEAE-Sephadex chromatography and contained no 14C. Methyl esterification of this compound with diazomethane proceeded in good yield and rendered the compound more amenable to chromatographic purification. The metabolite was isolated in several steps from rats dosed with 1 microgram of 1 alpha,25-dihydroxy[3 alpha-3H]vitamin D3. The metabolite was obtained in pure form as the methyl ester and was positively identified as 1 alpha,3 beta-dihydroxy-24-nor-9,10-seco-5,7,10(19)cholatrien-23-oic acid. The trivial name calcitroic acid is proposed for this major side-chain oxidized metabolite of 1,25-dihydroxyvitamin D3.", "contents": "Isolation and characterization of 1 alpha-hydroxy-23-carboxytetranorvitamin D: a major metabolite of 1,25-dihydroxyvitamin D3. The in vivo side-chain oxidation of 1 alpha,25-dihydroxyvitamin D3 was investigated by using a double-label radiotracer technique. Rats dosed with 1 alpha,25-dihydroxy-[3 alpha-3H]vitamin D3 and 1 alpha,25-dihydroxy[26,27-14C]vitamin D3 produced compounds with a high 3H/14C ratio. These compounds were found in sizable quantities in intestine and liver within 3 h after dosing. The major side-chain oxidized metabolite migrated as an acid on DEAE-Sephadex chromatography and contained no 14C. Methyl esterification of this compound with diazomethane proceeded in good yield and rendered the compound more amenable to chromatographic purification. The metabolite was isolated in several steps from rats dosed with 1 microgram of 1 alpha,25-dihydroxy[3 alpha-3H]vitamin D3. The metabolite was obtained in pure form as the methyl ester and was positively identified as 1 alpha,3 beta-dihydroxy-24-nor-9,10-seco-5,7,10(19)cholatrien-23-oic acid. The trivial name calcitroic acid is proposed for this major side-chain oxidized metabolite of 1,25-dihydroxyvitamin D3."}
{"id": "PMID:486409", "title": "Deoxyribonucleic acid binding studies on several new anthracycline antitumor antibiotics. Sequence preference and structure--activity relationships of marcellomycin and its analogues as compared to adriamycin.", "content": "The deoxyribonucleic acid (DNA) binding characteristics of adriamycin and several new anthracycline glycosides, including marcellomycin, aclacinomycin, rudolfomycin, musettamycin, and pyrromycin, have been studied. The fluorescence spectra were determined for all six anthracyclines, and the fluorescence quenching effects caused by interactions with the natural DNAs poly(dAdT)--poly(dAdT) and poly(dGdC) were characterized. Binding parameters were determined by Scatchard analyses of results obtained by spectrofluorometric titrations of anthracyclines with DNA. Consistent with earlier structure--activity relationship studies of nucleic acid synthesis inhibitory effects, the results demonstrate a correlation between the length of the glycosidic side chain and DNA binding affinity. In addition, the sugar residue 2-deoxyfucose appears to confer greater DNA binding ability than do the sugars rednosamine and cinerulose when present in the terminal position of the glycosidic side chain, also in agreement with earlier studies. The sequence preference of anthracycline--DNA interaction has been examined by using DNAs of varying GC content, including the naturally occurring calf thymus DNA (43% GC), Clostridium perfringens DNA (28% GC), and Micrococcus luteus DNA (72% GC) and the synthetic double-stranded copolymers poly(dGdC)--poly(dGdC) and poly(dAdT)--POLY(DAdT). The results demonstrate that although adriamycin shows an absolute requirement for GC sequences for DNA binding, marcellomycin and its analogues showed no such sequence requirement. Furthermore, an AT preference for DNA binding was demonstrated with marcellomycin and its analogues.", "contents": "Deoxyribonucleic acid binding studies on several new anthracycline antitumor antibiotics. Sequence preference and structure--activity relationships of marcellomycin and its analogues as compared to adriamycin. The deoxyribonucleic acid (DNA) binding characteristics of adriamycin and several new anthracycline glycosides, including marcellomycin, aclacinomycin, rudolfomycin, musettamycin, and pyrromycin, have been studied. The fluorescence spectra were determined for all six anthracyclines, and the fluorescence quenching effects caused by interactions with the natural DNAs poly(dAdT)--poly(dAdT) and poly(dGdC) were characterized. Binding parameters were determined by Scatchard analyses of results obtained by spectrofluorometric titrations of anthracyclines with DNA. Consistent with earlier structure--activity relationship studies of nucleic acid synthesis inhibitory effects, the results demonstrate a correlation between the length of the glycosidic side chain and DNA binding affinity. In addition, the sugar residue 2-deoxyfucose appears to confer greater DNA binding ability than do the sugars rednosamine and cinerulose when present in the terminal position of the glycosidic side chain, also in agreement with earlier studies. The sequence preference of anthracycline--DNA interaction has been examined by using DNAs of varying GC content, including the naturally occurring calf thymus DNA (43% GC), Clostridium perfringens DNA (28% GC), and Micrococcus luteus DNA (72% GC) and the synthetic double-stranded copolymers poly(dGdC)--poly(dGdC) and poly(dAdT)--POLY(DAdT). The results demonstrate that although adriamycin shows an absolute requirement for GC sequences for DNA binding, marcellomycin and its analogues showed no such sequence requirement. Furthermore, an AT preference for DNA binding was demonstrated with marcellomycin and its analogues."}
{"id": "PMID:486412", "title": "Cadmium-109 as a probe of the metal binding sites in horse liver alcohol dehydrogenase.", "content": "The noncatalytic and catalytic zinc atoms of horse liver alcohol dehydrogenase, [(LADH)Zn2Zn2] or LADH, have been replaced differentially with 109Cd by equilibrium dialysis, resulting in two new enzymatically active species, [(LADH)109Cd2Zn2] and [(LADH)109Cd2109Cd2]. The UV difference spectra of the cadmium enzymes vs. native [(LADH)Zn2Zn2] reveal maxima at 240 nm with molar absorptivities, delta epsilon 240, of 1.6 X 10(4) M-1 cm-1 per noncatalytic 109Cd atom and 0.9 X 10(4) M-1 cm-1 per catalytic 109Cd atom, consistent with coordination of the metals by four and two thiolate ligands, respectively, strikingly similar to the 250-nm charge-transfer absorbance in metallothionein. Carboxymethylation of the Cys-46 ligand to the catalytic metal in LADH presumably lowers the overall stability constant of the coordination complex and results in loss of catalytic 109Cd or catalytic cobalt but not catalytic zinc from the enzyme.", "contents": "Cadmium-109 as a probe of the metal binding sites in horse liver alcohol dehydrogenase. The noncatalytic and catalytic zinc atoms of horse liver alcohol dehydrogenase, [(LADH)Zn2Zn2] or LADH, have been replaced differentially with 109Cd by equilibrium dialysis, resulting in two new enzymatically active species, [(LADH)109Cd2Zn2] and [(LADH)109Cd2109Cd2]. The UV difference spectra of the cadmium enzymes vs. native [(LADH)Zn2Zn2] reveal maxima at 240 nm with molar absorptivities, delta epsilon 240, of 1.6 X 10(4) M-1 cm-1 per noncatalytic 109Cd atom and 0.9 X 10(4) M-1 cm-1 per catalytic 109Cd atom, consistent with coordination of the metals by four and two thiolate ligands, respectively, strikingly similar to the 250-nm charge-transfer absorbance in metallothionein. Carboxymethylation of the Cys-46 ligand to the catalytic metal in LADH presumably lowers the overall stability constant of the coordination complex and results in loss of catalytic 109Cd or catalytic cobalt but not catalytic zinc from the enzyme."}
{"id": "PMID:486413", "title": "Isolation and characterization of multiple forms of ovine pancreatic deoxyribonuclease. Chromatograhpic behavior of the enzyme on concanavalin A-agarose and carboxymethylcellulose columns.", "content": "A new procedure has been devised for the purification of ovine DNase, including (NH/4)2SO4 fractionation, two steps of CM-cellulose chromatography, concanavalin A-agarose chromatography, and gel filtration on Sephadex G--100. The enzyme, like bovine DNase, exhibits multiplicity due to changes in the primary structure and the sugar structure of the carbohydrate moiety. Unlike bovine DNase, ovine DNase does not have sialic acid in any of its multiple forms. Concanavalin A-agarose is useful in the purification of not only ovine but also bovine DNase. For ovine DNase, it is a necessary and key step of purification; for bovine DNase, it can be used to purify commercial preparations of DNase free from proteases in a single step as judged by its stability in Ca2+-free media at pH 8.0. The purified enzyme has a specific activity equal to that of a highly purified DNase and presumably contains predominantly DNases A and C. Two of the four forms of ovine DNase have been purified to apparent homogeneity and subjected to chemical analysis. The present results show that bovine and ovine DNases have indistinguishable molecular weights and identical end groups, suggesting that they may have the same number of amino acid residues. The amino acid composition indicates that two enzymes may have six residues of amino acids subject to substitution which can be explained by single base changes in their genetic code words. Amino acid analyses also indicate that the most likely difference between two forms of ovine DNase is the substitution of Leu for Arg.", "contents": "Isolation and characterization of multiple forms of ovine pancreatic deoxyribonuclease. Chromatograhpic behavior of the enzyme on concanavalin A-agarose and carboxymethylcellulose columns. A new procedure has been devised for the purification of ovine DNase, including (NH/4)2SO4 fractionation, two steps of CM-cellulose chromatography, concanavalin A-agarose chromatography, and gel filtration on Sephadex G--100. The enzyme, like bovine DNase, exhibits multiplicity due to changes in the primary structure and the sugar structure of the carbohydrate moiety. Unlike bovine DNase, ovine DNase does not have sialic acid in any of its multiple forms. Concanavalin A-agarose is useful in the purification of not only ovine but also bovine DNase. For ovine DNase, it is a necessary and key step of purification; for bovine DNase, it can be used to purify commercial preparations of DNase free from proteases in a single step as judged by its stability in Ca2+-free media at pH 8.0. The purified enzyme has a specific activity equal to that of a highly purified DNase and presumably contains predominantly DNases A and C. Two of the four forms of ovine DNase have been purified to apparent homogeneity and subjected to chemical analysis. The present results show that bovine and ovine DNases have indistinguishable molecular weights and identical end groups, suggesting that they may have the same number of amino acid residues. The amino acid composition indicates that two enzymes may have six residues of amino acids subject to substitution which can be explained by single base changes in their genetic code words. Amino acid analyses also indicate that the most likely difference between two forms of ovine DNase is the substitution of Leu for Arg."}
{"id": "PMID:486415", "title": "Dependence of the conformation of the polar head groups of phosphatidylcholine on its packing in bilayers. Nuclear magnetic resonance studies on the effect of the binding of lanthanide ions.", "content": "Proton magnetic resonance spectra of vesicles of various sizes composed of egg phosphatidylcholine (PC) with varying concentrations of cholesterol differed in the apparent line width of the signal of the methylene protons of PC (delta v1/2). They also varied in the extent of lanthanide-induced shifts of the 31P and 1H NMR signals of the corresponding nuclei of the polar head groups located on the outer surface of the vesicles (delta delta). The differences in the lanthanide-induced shifts of the 31P signals are fully accounted for by the ratio between the externally added lanthanide and the number of PC head groups available for interaction with the lanthanide ions. This was not the case ofr the changes in the 1H NMR spectra. Here delta delta decreased with increasing delta v1/2, suggesting that the packing of the PC paraffinic chaings in the bilayer affects the conformation of the polar head groups; tightening of the packing probably results in a more extended conformation of the head groups. This conclusion is also supported by the larger effect lanthanides have on the 1H chemical shift of the choline head groups on the outer surface of small unilamellar vesicles as compared to groups on the inner, tighter packed layer.", "contents": "Dependence of the conformation of the polar head groups of phosphatidylcholine on its packing in bilayers. Nuclear magnetic resonance studies on the effect of the binding of lanthanide ions. Proton magnetic resonance spectra of vesicles of various sizes composed of egg phosphatidylcholine (PC) with varying concentrations of cholesterol differed in the apparent line width of the signal of the methylene protons of PC (delta v1/2). They also varied in the extent of lanthanide-induced shifts of the 31P and 1H NMR signals of the corresponding nuclei of the polar head groups located on the outer surface of the vesicles (delta delta). The differences in the lanthanide-induced shifts of the 31P signals are fully accounted for by the ratio between the externally added lanthanide and the number of PC head groups available for interaction with the lanthanide ions. This was not the case ofr the changes in the 1H NMR spectra. Here delta delta decreased with increasing delta v1/2, suggesting that the packing of the PC paraffinic chaings in the bilayer affects the conformation of the polar head groups; tightening of the packing probably results in a more extended conformation of the head groups. This conclusion is also supported by the larger effect lanthanides have on the 1H chemical shift of the choline head groups on the outer surface of small unilamellar vesicles as compared to groups on the inner, tighter packed layer."}
{"id": "PMID:486416", "title": "Interactions of melittin, a preprotein model, with detergents.", "content": "Bee venom melittin is a water-soluble tetramer of identical polypeptide chains. Each chain has 26 residues. The 20 N-terminal residues are hydrophobic and the 6 C-terminal residues are basic. Melittin has been shown to integrate into natural and synthetic membranes and to lyse a wide variety of cells. To understand how a water-soluble protein can spontaneously partition into a membrane, we have studied the interaction of melittin with micelles of deoxycholate (DOC), Brij 58, and sodium dodecyl sulfate (NaDodSO4). Circular dichroism spectra showed that NaDodSO4, an ionic detergent, and Brij 58, a nonionic detergent, caused similar major changes in the protein's conformation. Gel filtration studies revealed that melittin forms mixed micelles with either Brij or DOC. The melittin-DOC mixed micelles have 2 mol of DOC per mol of melittin. Cross-linking studies with dimethyl suberimidate confirmed that the protein is a tetramer and showed that it becomes monomeric either in mixed micelles with Brij or DOC or in butanol. Despite this major structural change of melittin in the presence of an amphiphile, the covalently cross-linked form is as active in human erythrocyte lysis as the native protein.", "contents": "Interactions of melittin, a preprotein model, with detergents. Bee venom melittin is a water-soluble tetramer of identical polypeptide chains. Each chain has 26 residues. The 20 N-terminal residues are hydrophobic and the 6 C-terminal residues are basic. Melittin has been shown to integrate into natural and synthetic membranes and to lyse a wide variety of cells. To understand how a water-soluble protein can spontaneously partition into a membrane, we have studied the interaction of melittin with micelles of deoxycholate (DOC), Brij 58, and sodium dodecyl sulfate (NaDodSO4). Circular dichroism spectra showed that NaDodSO4, an ionic detergent, and Brij 58, a nonionic detergent, caused similar major changes in the protein's conformation. Gel filtration studies revealed that melittin forms mixed micelles with either Brij or DOC. The melittin-DOC mixed micelles have 2 mol of DOC per mol of melittin. Cross-linking studies with dimethyl suberimidate confirmed that the protein is a tetramer and showed that it becomes monomeric either in mixed micelles with Brij or DOC or in butanol. Despite this major structural change of melittin in the presence of an amphiphile, the covalently cross-linked form is as active in human erythrocyte lysis as the native protein."}
{"id": "PMID:486417", "title": "Quantitative assessment of the noncovalent inhibition of sickle hemoglobin gelation by phenyl derivatives and other known agents.", "content": "The ability of a variety of phenyl derivatives to inhibit sickle cell hemoglobin gelation was placed on a quantitative scale by parallel equilibrium and kinetic assays. Modifications of the phenyl ring studied include polar, nonpolar, and charged substituents, added aromatic rings, and loss of aromaticity. Other noncovalent inhibitors previously reported to have high potency were measured and placed on the same quantitative scale. Some phenyl derivatives were found to be as effective an any other known noncovalent antigelling agent. The phenyl compounds penetrate easily into red cells, and their potency is tolerant to chemical modification, which holds out the possibility of designing low-toxicity derivatives. On the negative side, the level of potency obtainable appears to be inadequate for clinical use. The best phenyl inhibitors display a functionally defined inhibitory constant (K1) of 75 mM, and it can be estimated that inhibitor concentrations over 20 mM would be necessary to obtain minimal clinically significant benefit. Furthermore, with the variety of modifications tested here, no impressive increase in activity could be achieved over that found in the simplest phenyl compounds.", "contents": "Quantitative assessment of the noncovalent inhibition of sickle hemoglobin gelation by phenyl derivatives and other known agents. The ability of a variety of phenyl derivatives to inhibit sickle cell hemoglobin gelation was placed on a quantitative scale by parallel equilibrium and kinetic assays. Modifications of the phenyl ring studied include polar, nonpolar, and charged substituents, added aromatic rings, and loss of aromaticity. Other noncovalent inhibitors previously reported to have high potency were measured and placed on the same quantitative scale. Some phenyl derivatives were found to be as effective an any other known noncovalent antigelling agent. The phenyl compounds penetrate easily into red cells, and their potency is tolerant to chemical modification, which holds out the possibility of designing low-toxicity derivatives. On the negative side, the level of potency obtainable appears to be inadequate for clinical use. The best phenyl inhibitors display a functionally defined inhibitory constant (K1) of 75 mM, and it can be estimated that inhibitor concentrations over 20 mM would be necessary to obtain minimal clinically significant benefit. Furthermore, with the variety of modifications tested here, no impressive increase in activity could be achieved over that found in the simplest phenyl compounds."}
{"id": "PMID:486418", "title": "Fractionation of constituents of ribonucleoproteins containing heterogeneous nuclear ribonucleic acid.", "content": "A method of fractionation of hnRNP constituents adaptable to large-scale preparation is presented. It is based on differential resistance to salt dissociation of the two classes of units of hnRNP, the 30--50S monoparticles and the heterogeneous complexes. The monoparticle proteins were released from hnRNP by 0.4 M NaCl. They were separated from the salt-resistant RNP corresponding to the heterogeneous complexes in three steps: chromatography on DEAE-cellulose, high-speed centrifugation, and Bio-Gel chromatography. The latter chromatography permitted a first fractionation of monoparticle proteins according to molecular weight. Such fractions may serve for purification of individual proteins of molecular weight below 80 000. After the two first steps, two fractions of salt-resistant RNP were obtained. In addition to heterogeneous RNA up to 30 S, small nuclear RNAs were detected which represented 6% of total RNA. The protein pattern was complex, and no clear-cut segregation of groups of proteins could be observed between the two fractions. They were both highly enriched in phosphoproteins as compared to nomoparticle proteins. In another fraction corresponding to the void volume of Bio-Gel chromatography, one-third of the RNA was small nuclear RNA. It is suggested that this fraction contains snRNP in addition to free proteins of molecular weight above 80 000 and to salt-resistant RNP similar to those described above but of small size.", "contents": "Fractionation of constituents of ribonucleoproteins containing heterogeneous nuclear ribonucleic acid. A method of fractionation of hnRNP constituents adaptable to large-scale preparation is presented. It is based on differential resistance to salt dissociation of the two classes of units of hnRNP, the 30--50S monoparticles and the heterogeneous complexes. The monoparticle proteins were released from hnRNP by 0.4 M NaCl. They were separated from the salt-resistant RNP corresponding to the heterogeneous complexes in three steps: chromatography on DEAE-cellulose, high-speed centrifugation, and Bio-Gel chromatography. The latter chromatography permitted a first fractionation of monoparticle proteins according to molecular weight. Such fractions may serve for purification of individual proteins of molecular weight below 80 000. After the two first steps, two fractions of salt-resistant RNP were obtained. In addition to heterogeneous RNA up to 30 S, small nuclear RNAs were detected which represented 6% of total RNA. The protein pattern was complex, and no clear-cut segregation of groups of proteins could be observed between the two fractions. They were both highly enriched in phosphoproteins as compared to nomoparticle proteins. In another fraction corresponding to the void volume of Bio-Gel chromatography, one-third of the RNA was small nuclear RNA. It is suggested that this fraction contains snRNP in addition to free proteins of molecular weight above 80 000 and to salt-resistant RNP similar to those described above but of small size."}
{"id": "PMID:486420", "title": "Dilatometric study of binary mixtures of phosphatidylcholines.", "content": "Volumes of lipid dispersions as a function of temperature have been measured for two different kinds of binary mixtures of lecithins, (1) DMPC and DSPC and (2) DMPC and DC20PC. Emphasis was placed on DMPC-rich compositions so as to resolve ambiguities regarding solid-phase immiscibility in DMPC-DSPC mixtures. Special attention has been paid to problems of equilibration in the low-temperature phase and to methods of mixing the lipids. We find that there is no solid-solid immiscibility in DMPC-DSPC mixtures, although this system is close to exhibiting such immiscibility, and that DMPC-DC20PC mixtures exhibit pronounced solid immiscibility.", "contents": "Dilatometric study of binary mixtures of phosphatidylcholines. Volumes of lipid dispersions as a function of temperature have been measured for two different kinds of binary mixtures of lecithins, (1) DMPC and DSPC and (2) DMPC and DC20PC. Emphasis was placed on DMPC-rich compositions so as to resolve ambiguities regarding solid-phase immiscibility in DMPC-DSPC mixtures. Special attention has been paid to problems of equilibration in the low-temperature phase and to methods of mixing the lipids. We find that there is no solid-solid immiscibility in DMPC-DSPC mixtures, although this system is close to exhibiting such immiscibility, and that DMPC-DC20PC mixtures exhibit pronounced solid immiscibility."}
{"id": "PMID:486421", "title": "Electronic transitions in the isoalloxazine ring and orientation of flavins in model membranes studied by polarized light spectroscopy.", "content": "The orientation of flavin mononucleotide (FMN) in model membranes and the directions of the transition moments of the first three bands in the electronic absorption spectrum of the oxidized form of the isoalloxazine ring have been determined by means of linear dichroism and polarized fluorescence spectroscopy. Measured counterclockwise relative to the axis connecting the two nitrogens in the central ring (considered positive when going in the direction from -CN less than to greater than or equal to N), these angles are 58 +/- 4 degrees (450-nm band), 97 +/- 3 degrees (350-nm band), and 119 +/- 2 degrees (260-nm band).", "contents": "Electronic transitions in the isoalloxazine ring and orientation of flavins in model membranes studied by polarized light spectroscopy. The orientation of flavin mononucleotide (FMN) in model membranes and the directions of the transition moments of the first three bands in the electronic absorption spectrum of the oxidized form of the isoalloxazine ring have been determined by means of linear dichroism and polarized fluorescence spectroscopy. Measured counterclockwise relative to the axis connecting the two nitrogens in the central ring (considered positive when going in the direction from -CN less than to greater than or equal to N), these angles are 58 +/- 4 degrees (450-nm band), 97 +/- 3 degrees (350-nm band), and 119 +/- 2 degrees (260-nm band)."}
{"id": "PMID:486422", "title": "Comparison of complexity and diversity of polyadenylated polysomal and informosomal messenger ribonucleic acid from Chinese hamster cells.", "content": "The sequence complexity and relative abundance of cytoplasmic polyadenylated polysomal (ribosome-bound) mRNA and cytoplasmic polyadenylated informosomal (ribosome-free) mRNA were analyzed in exponentially growing Chinese hamster cells (line CHO) using the technique of cDNA hybridization to excess poly(A)+ mRNA. Polysomal and informosomal mRNAs had similar complexities ( approximately 8300 mRNA species), but both the fraction of mRNA and the number of sequences comprising the mRNA abundance classes were different. Heterologous annealing reactions showed that all of the mRNA sequences detected were shared by the polysomal and informosomal mRNAs. However, the most abundant informosomal mRNA component was considerably different from the most abundant polysomal mRNA component. For a more detailed analysis, cDNA complementary to the most abundant informosomal and polysomal mRNAs was isolated. By use of the fractionated cDNA, it could be demonstrated that the most abundant informosomal mRNA sequences were distributed in the polysomal mRNA with an approximately fivefold reduction in relative frequency. These results are not compatible with models postulating translational control of gene expression by the complete sequestering of some mRNA sequences in an untranslatable form in the cytoplasm. The data are, however, consistent with models encompassing differential rates of initiation on the polysome and/or preferential affinity of some mRNAs for initiation factors.", "contents": "Comparison of complexity and diversity of polyadenylated polysomal and informosomal messenger ribonucleic acid from Chinese hamster cells. The sequence complexity and relative abundance of cytoplasmic polyadenylated polysomal (ribosome-bound) mRNA and cytoplasmic polyadenylated informosomal (ribosome-free) mRNA were analyzed in exponentially growing Chinese hamster cells (line CHO) using the technique of cDNA hybridization to excess poly(A)+ mRNA. Polysomal and informosomal mRNAs had similar complexities ( approximately 8300 mRNA species), but both the fraction of mRNA and the number of sequences comprising the mRNA abundance classes were different. Heterologous annealing reactions showed that all of the mRNA sequences detected were shared by the polysomal and informosomal mRNAs. However, the most abundant informosomal mRNA component was considerably different from the most abundant polysomal mRNA component. For a more detailed analysis, cDNA complementary to the most abundant informosomal and polysomal mRNAs was isolated. By use of the fractionated cDNA, it could be demonstrated that the most abundant informosomal mRNA sequences were distributed in the polysomal mRNA with an approximately fivefold reduction in relative frequency. These results are not compatible with models postulating translational control of gene expression by the complete sequestering of some mRNA sequences in an untranslatable form in the cytoplasm. The data are, however, consistent with models encompassing differential rates of initiation on the polysome and/or preferential affinity of some mRNAs for initiation factors."}
{"id": "PMID:486423", "title": "Diaspirins that cross-link beta chains of hemoglobin: bis(3,5-dibromosalicyl) succinate and bis(3,5-dibromosalicyl) fumarate.", "content": "Two double-headed aspirins, bis(3,5-dibromosalicyl) succinate and bis(3,5-dibromosalicyl) fumarate, have been found to be potent acylating agents of intracellular hemoglobin (A or S) in vitro. Furthermore, each of these reagents cross-links beta chains of hemoglobin, probably at the beta cleft. The modified hemoglobins show increased oxygen affinities and reduced gelation or sickling tendencies.", "contents": "Diaspirins that cross-link beta chains of hemoglobin: bis(3,5-dibromosalicyl) succinate and bis(3,5-dibromosalicyl) fumarate. Two double-headed aspirins, bis(3,5-dibromosalicyl) succinate and bis(3,5-dibromosalicyl) fumarate, have been found to be potent acylating agents of intracellular hemoglobin (A or S) in vitro. Furthermore, each of these reagents cross-links beta chains of hemoglobin, probably at the beta cleft. The modified hemoglobins show increased oxygen affinities and reduced gelation or sickling tendencies."}
{"id": "PMID:486424", "title": "Structural basis for the anticoagulant activity of heparin. 1. Relationship to the number of charged groups.", "content": "This study was undertaken to provide further information concerning the chemical heterogeneity of heparins and the relationships between the anticoagulant activity (USP assay) and the anionic density of the heparin. A sample of commercial heparin was fractionated into 13 fractions by sequential extraction in a two-phase system of 1-butanol-aqueous NaCl containing excess hexadecylpyridinium chloride. The anionic density distribution was characterized by the fractional distribution of uronate among the fractions. The fractions were characterized by several molar ratios of constituents, molecular weight, charge density, and anticoagulant activity in recalcified sheep plasma. The heparin was broadly distributed among the last 10 fractions; the first three contained impurities which were completely separated from the heparin fractions. The heparin fractions differ systematically in anionic density but are of substantially the same molecular weight. Anticoagulant activity increased markedly with anionic density, ranging from 81 units/mg for the heparin fraction with the lowest anionic density up to a high of 243 units/mg. The relationship between anticoagulant activity and either anionic density or its square is nonlinear. However, in the latter case an initial linear relationship was observed for anticoagulant activities of less than 200 units/mg.", "contents": "Structural basis for the anticoagulant activity of heparin. 1. Relationship to the number of charged groups. This study was undertaken to provide further information concerning the chemical heterogeneity of heparins and the relationships between the anticoagulant activity (USP assay) and the anionic density of the heparin. A sample of commercial heparin was fractionated into 13 fractions by sequential extraction in a two-phase system of 1-butanol-aqueous NaCl containing excess hexadecylpyridinium chloride. The anionic density distribution was characterized by the fractional distribution of uronate among the fractions. The fractions were characterized by several molar ratios of constituents, molecular weight, charge density, and anticoagulant activity in recalcified sheep plasma. The heparin was broadly distributed among the last 10 fractions; the first three contained impurities which were completely separated from the heparin fractions. The heparin fractions differ systematically in anionic density but are of substantially the same molecular weight. Anticoagulant activity increased markedly with anionic density, ranging from 81 units/mg for the heparin fraction with the lowest anionic density up to a high of 243 units/mg. The relationship between anticoagulant activity and either anionic density or its square is nonlinear. However, in the latter case an initial linear relationship was observed for anticoagulant activities of less than 200 units/mg."}
{"id": "PMID:486425", "title": "Structural basis for the anticoagulant activity of heparin. 2. Relationship of anticoagulant activity to the thermodynamics and fluorescence fading kinetics of acridine orange-heparin complexes.", "content": "Complexing heparin or dermatan sulfate with the fluorescent probe acridine orange provides a means of studying electrostatic as well as static and dynamic conformational aspects of these glycosaminoglycans via the thermodynamic and photochemical (fluorescence fading) properties of these complexes. The cooperative binding constants (Kq), fluorescence fading rate parameters (r''), and anticoagulant activities of heparins fractionated according to anionic density all showed qualitatively the same dependence upon anionic density. When Kq and r'' were plotted against anticoagulant activity, empirical relationships were observed. Interestingly, the corresponding values for unfractionated dermatan sulfate fell on the lines defined by the heparin fractions. Temperature-dependence, studies demonstrated that differences in fading rate observed for heparins of different anionic densities are entropic in origin and reflect differences in the ability to assume a special configuration. Differences in activation entropy for fluorescence fading can be empirically correlated with anticoagulant activity. The latter correlation suggests a physical similarity in the roles played by anionic density in both fluorescence fading and anticoagulant activity.", "contents": "Structural basis for the anticoagulant activity of heparin. 2. Relationship of anticoagulant activity to the thermodynamics and fluorescence fading kinetics of acridine orange-heparin complexes. Complexing heparin or dermatan sulfate with the fluorescent probe acridine orange provides a means of studying electrostatic as well as static and dynamic conformational aspects of these glycosaminoglycans via the thermodynamic and photochemical (fluorescence fading) properties of these complexes. The cooperative binding constants (Kq), fluorescence fading rate parameters (r''), and anticoagulant activities of heparins fractionated according to anionic density all showed qualitatively the same dependence upon anionic density. When Kq and r'' were plotted against anticoagulant activity, empirical relationships were observed. Interestingly, the corresponding values for unfractionated dermatan sulfate fell on the lines defined by the heparin fractions. Temperature-dependence, studies demonstrated that differences in fading rate observed for heparins of different anionic densities are entropic in origin and reflect differences in the ability to assume a special configuration. Differences in activation entropy for fluorescence fading can be empirically correlated with anticoagulant activity. The latter correlation suggests a physical similarity in the roles played by anionic density in both fluorescence fading and anticoagulant activity."}
{"id": "PMID:486428", "title": "Single-step isolation and resolution of pancreatic carboxypeptidases A and B.", "content": "Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). The affinity ligand was synthesized from DL-benzylsuccinic acid, purified, and characterized by UV absorption and NMR spectroscopy. Both enzymes from the various species were homogeneous by NaDodSO4-polyacrylamide gel electrophoresis and displayed high specific activities. No cross contamination of one enzyme species with the other was found. The ease of synthesis of the ligand from its commercially available precursor, its stability, and the mild elution conditions render CABS-Sepharose an excellent affinity support for the single-column isolation of both carboxypeptidases A and B. The procedures extend the utility of this resin previously demonstrated for carboxypeptidase A from human pancreatic juice [Peterson, L. M., Sokolovsky, M., & Vallee, B. L. (1976) Biochemistry, 15, 2501]. The use of CABS-Sepharose as a general affinity matrix for the isolation of metallocarboxypeptidases is suggested.", "contents": "Single-step isolation and resolution of pancreatic carboxypeptidases A and B. Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). The affinity ligand was synthesized from DL-benzylsuccinic acid, purified, and characterized by UV absorption and NMR spectroscopy. Both enzymes from the various species were homogeneous by NaDodSO4-polyacrylamide gel electrophoresis and displayed high specific activities. No cross contamination of one enzyme species with the other was found. The ease of synthesis of the ligand from its commercially available precursor, its stability, and the mild elution conditions render CABS-Sepharose an excellent affinity support for the single-column isolation of both carboxypeptidases A and B. The procedures extend the utility of this resin previously demonstrated for carboxypeptidase A from human pancreatic juice [Peterson, L. M., Sokolovsky, M., & Vallee, B. L. (1976) Biochemistry, 15, 2501]. The use of CABS-Sepharose as a general affinity matrix for the isolation of metallocarboxypeptidases is suggested."}
{"id": "PMID:486430", "title": "Phospholipid-binding properties of bovine factor V and factor Va.", "content": "Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid binding of prothrombin and factor X were also studied. The average values obtained for the dissociation constants (Kd) and lipid to protein ratio at saturation, moles/mole (n), for prothrombin (Kd = 2.3 X 10(-6) M, n = 104) and factor X (Kd = 2.5 X 10(-6) M, n = 46) binding to vesicles containing 25% Folch fraction III and 75% phosphatidylcholine in the presence of 2 mM Ca2+ were in agreement with those reported in the literature. The average factor V and factor Va values for the dissociation constants and lipid to protein ratio at saturation (moles/mole) were Kd = 7.2 X 10(-8) M and n = 270 for factor V and Kd = 4.4 X 10(-7) M and n = 76 for factor Va. In contrast to prothrombin and factor X, factor V and factor Va demonstrated Ca2+-independent lipid binding. In addition, the number of factor V and factor Va molecules bound per vesicle was found to be dependent both on the phosphatidylserine content of the vesicle and the ionic strength of the buffer.", "contents": "Phospholipid-binding properties of bovine factor V and factor Va. Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid binding of prothrombin and factor X were also studied. The average values obtained for the dissociation constants (Kd) and lipid to protein ratio at saturation, moles/mole (n), for prothrombin (Kd = 2.3 X 10(-6) M, n = 104) and factor X (Kd = 2.5 X 10(-6) M, n = 46) binding to vesicles containing 25% Folch fraction III and 75% phosphatidylcholine in the presence of 2 mM Ca2+ were in agreement with those reported in the literature. The average factor V and factor Va values for the dissociation constants and lipid to protein ratio at saturation (moles/mole) were Kd = 7.2 X 10(-8) M and n = 270 for factor V and Kd = 4.4 X 10(-7) M and n = 76 for factor Va. In contrast to prothrombin and factor X, factor V and factor Va demonstrated Ca2+-independent lipid binding. In addition, the number of factor V and factor Va molecules bound per vesicle was found to be dependent both on the phosphatidylserine content of the vesicle and the ionic strength of the buffer."}
{"id": "PMID:486431", "title": "Preparation of the bifunctional enzyme ribonuclease-deoxyribonuclease by cross-linkage.", "content": "Protease-free bovine pancreatic deoxyribonuclease (DNase) (1.6 X 10(-4) mmol) was thiolated on the NH2 groups with N-acetyl-DL-homocysteine thiolactone (2.4 X 10(-2) mmol) at pH 10.5 with imidazole (2.4 X 10(-2) mmol) as the catalyst in the presence of 4,4'-dithiodipyridine (4.2 X 10(-2) mmol). The product obtained after 16 h at 4 degrees C, 2-acetamido-4-(4'-dithiopyridyl)butyryl-DNase, isolated by gel filtration, contained an average of 0.87 +/- 0.13 mol of mixed disulfide per mol of DNase. Ribonuclease (RNase) was thiolated in a similar manner, but under N2 in the absence of 4,4'-dithiodipyridine. The protein N-acetylhomocysteinyl-RNase contained on the average 0.94 +/- 0.11 mol of sulfhydryl groups per mol of RNase. The coupling of RNase ot DNase was accomplished by thiol-disulfide interchange at pH 6.2 and 25 degrees C for 90 min. The hybrid enzyme (yield 25--33%, based upon the DNase derivative used) was freed from unreacted DNase, RNase, and homodimers by gel filtration, affinity chromatography, and salting-out chromatography. The purified enzyme contained one molecule each of DNase and RNase and hydrolyzed thymus deoxyribonucleic acid (DNA) and yeast or transfer ribonucleic acid (RNA) with 75 and 40% of the efficiencies, respectively, of the parent enzymes. The RNA strand of the hybrid substrate, phage f1 DNA-[3H]RNA, prepared from phage DNA with RNA polymerase, was hydrolyzed rapidly by the hybrid enzyme but was not hydrolyzed by RNase alone. A conjugate of the two enzymes offers the possibility in vivo of delivering two enzymes that differ in size, charge, and biological function to the same site at the same time.", "contents": "Preparation of the bifunctional enzyme ribonuclease-deoxyribonuclease by cross-linkage. Protease-free bovine pancreatic deoxyribonuclease (DNase) (1.6 X 10(-4) mmol) was thiolated on the NH2 groups with N-acetyl-DL-homocysteine thiolactone (2.4 X 10(-2) mmol) at pH 10.5 with imidazole (2.4 X 10(-2) mmol) as the catalyst in the presence of 4,4'-dithiodipyridine (4.2 X 10(-2) mmol). The product obtained after 16 h at 4 degrees C, 2-acetamido-4-(4'-dithiopyridyl)butyryl-DNase, isolated by gel filtration, contained an average of 0.87 +/- 0.13 mol of mixed disulfide per mol of DNase. Ribonuclease (RNase) was thiolated in a similar manner, but under N2 in the absence of 4,4'-dithiodipyridine. The protein N-acetylhomocysteinyl-RNase contained on the average 0.94 +/- 0.11 mol of sulfhydryl groups per mol of RNase. The coupling of RNase ot DNase was accomplished by thiol-disulfide interchange at pH 6.2 and 25 degrees C for 90 min. The hybrid enzyme (yield 25--33%, based upon the DNase derivative used) was freed from unreacted DNase, RNase, and homodimers by gel filtration, affinity chromatography, and salting-out chromatography. The purified enzyme contained one molecule each of DNase and RNase and hydrolyzed thymus deoxyribonucleic acid (DNA) and yeast or transfer ribonucleic acid (RNA) with 75 and 40% of the efficiencies, respectively, of the parent enzymes. The RNA strand of the hybrid substrate, phage f1 DNA-[3H]RNA, prepared from phage DNA with RNA polymerase, was hydrolyzed rapidly by the hybrid enzyme but was not hydrolyzed by RNase alone. A conjugate of the two enzymes offers the possibility in vivo of delivering two enzymes that differ in size, charge, and biological function to the same site at the same time."}
{"id": "PMID:486432", "title": "Effects of light intensity on membrane differentiation in Rhodopseudomonas capsulata.", "content": "Cells of Rhodopseudomonas capsulata, strain 37b4, leu-, precultivated anaerobically under low light intensity, were exposed to high light intensity (2000 W.m-2). The cells grew with a mass doubling time of 3 h. The synthesis of bacteriochlorophyll (BChl) began after two doublings of cell mass. Reaction center and light-harvesting BChl I (B-875) were the main constituents of the photosynthetic apparatus incorporated into the membrane. The size of the photosynthetic unit (total BChl/reaction center) decreased and light-harvesting BChl I became the dominating BChl species. Concomitant with the appearance of the different spectral forms of BChl the respective proteins were incorporated into the membrane, i.e. the three reaction center polypeptides, the polypeptide associated with light-harvesting BChl I, the two polypeptides associated with BChl II. A polypeptide of an apparent molecular weight of 45 000 was also incorporated. A lowering of the light intensity to 7 W.m-2 resulted in a lag phase of growth for 6 h. Afterwards, the time for doubling of cell mass was 11 h. The concentration of all three BChl complexes (reaction center, light-harvesting BChl I and II complexes)/cell and per membrane protein increased immediately. Also the size of the photosynthetic unit and the amount of intracytoplasmic membranes/cell increased. The activities of photophosphorylation, succinate dehydrogenase, NADH dehydrogenase and NADH oxidation (respiratory chain)/membrane protein are higher in membrane preparations isolated from cells grown at high light intensities than in such preparations from cells grown at low light intensities.", "contents": "Effects of light intensity on membrane differentiation in Rhodopseudomonas capsulata. Cells of Rhodopseudomonas capsulata, strain 37b4, leu-, precultivated anaerobically under low light intensity, were exposed to high light intensity (2000 W.m-2). The cells grew with a mass doubling time of 3 h. The synthesis of bacteriochlorophyll (BChl) began after two doublings of cell mass. Reaction center and light-harvesting BChl I (B-875) were the main constituents of the photosynthetic apparatus incorporated into the membrane. The size of the photosynthetic unit (total BChl/reaction center) decreased and light-harvesting BChl I became the dominating BChl species. Concomitant with the appearance of the different spectral forms of BChl the respective proteins were incorporated into the membrane, i.e. the three reaction center polypeptides, the polypeptide associated with light-harvesting BChl I, the two polypeptides associated with BChl II. A polypeptide of an apparent molecular weight of 45 000 was also incorporated. A lowering of the light intensity to 7 W.m-2 resulted in a lag phase of growth for 6 h. Afterwards, the time for doubling of cell mass was 11 h. The concentration of all three BChl complexes (reaction center, light-harvesting BChl I and II complexes)/cell and per membrane protein increased immediately. Also the size of the photosynthetic unit and the amount of intracytoplasmic membranes/cell increased. The activities of photophosphorylation, succinate dehydrogenase, NADH dehydrogenase and NADH oxidation (respiratory chain)/membrane protein are higher in membrane preparations isolated from cells grown at high light intensities than in such preparations from cells grown at low light intensities."}
{"id": "PMID:486433", "title": "Bacteriorhodopsin in liposomes. I. A description using irreversible thermodynamics.", "content": "A comprehensive description of light-induced ion transport in bacteriorhodopsin liposomes is presented. Linear irreversible thermodynamics and the chemiosmotic theory serve as theoretical bases for the formulation of a limited number of fundamental equations. In these equations mechanistic parameters characterize the dependence of ion movement and flux through the photochemical cycle of bacteriorhodopsin on electrochemical potential differences and a so-called light affinity. By making appropriate steady-state assummptions and carrying out mathematical reduction experimentally testable expressions, still containing the mechanistic parameters, are obtained. In the accompanying article rigid trials to falsify these expressions are shown to be unsuccessful.", "contents": "Bacteriorhodopsin in liposomes. I. A description using irreversible thermodynamics. A comprehensive description of light-induced ion transport in bacteriorhodopsin liposomes is presented. Linear irreversible thermodynamics and the chemiosmotic theory serve as theoretical bases for the formulation of a limited number of fundamental equations. In these equations mechanistic parameters characterize the dependence of ion movement and flux through the photochemical cycle of bacteriorhodopsin on electrochemical potential differences and a so-called light affinity. By making appropriate steady-state assummptions and carrying out mathematical reduction experimentally testable expressions, still containing the mechanistic parameters, are obtained. In the accompanying article rigid trials to falsify these expressions are shown to be unsuccessful."}
{"id": "PMID:486434", "title": "Bacteriorhodopsin in liposomes. II. Experimental evidence in support of a theoretical model.", "content": "In the preceding article equations describing relevant ion flows in illuminated suspensions of bacteriorhodopsin liposomes have been derived. Here these equations are subjected to experimental tests. Changes in permeability characteristics of the liposomal membrane are brought about by addition of specific ionophores and change of medium composition. Using light-driven proton uptake and electrochemical potential differences for protons across the membrane as observation parameters, ridig attempts to falsify the derived equations are unsuccessful. Agreement between equations and experimental results is established on the point of: (i) the antagonistic effect of valinomycin and nigericin on the two components of the proton-motive force, (ii) the time dependence of the changes in transmembrane electrical and chemical potential differences after the onset of illumination. In three independent experimental systems evidence was obtained for the correctness of the postulated dependence of the turnover rate of the photochemical cycle on back pressure by the transmembrane electrochemical potential difference for protons.", "contents": "Bacteriorhodopsin in liposomes. II. Experimental evidence in support of a theoretical model. In the preceding article equations describing relevant ion flows in illuminated suspensions of bacteriorhodopsin liposomes have been derived. Here these equations are subjected to experimental tests. Changes in permeability characteristics of the liposomal membrane are brought about by addition of specific ionophores and change of medium composition. Using light-driven proton uptake and electrochemical potential differences for protons across the membrane as observation parameters, ridig attempts to falsify the derived equations are unsuccessful. Agreement between equations and experimental results is established on the point of: (i) the antagonistic effect of valinomycin and nigericin on the two components of the proton-motive force, (ii) the time dependence of the changes in transmembrane electrical and chemical potential differences after the onset of illumination. In three independent experimental systems evidence was obtained for the correctness of the postulated dependence of the turnover rate of the photochemical cycle on back pressure by the transmembrane electrochemical potential difference for protons."}
{"id": "PMID:486435", "title": "Vectorial redox reactions of physiological quinones. I. Requirement of a minimum length of the isoprenoid side chain.", "content": "Physiological quinones carrying isoprenoid side chains have been compared with homologues lacking the side chain, for their ability to carry electrons and protons from dithionite to ferricyanide, trapped in liposomes. Six differential observations were made: (1) Plastoquinone and ubiquinones, with a side chain of more than two isoprene units, are by far better mediators than their short-chain homologues. Also other benzoquinones lacking a long side chain are poor catalysts, except dimethyl-methylenedioxy-p-benzoquinone, a highly autooxidizable compound. Tocopherol is a good catalyst. (2) Vitamin K-1 and K-2 are poor mediators compared to vitamin K-3. (3) The reaction catalyzed by quinones carrying long isoprenoid side chains has an about three-fold higher activation energy, irrespective of the catalytic efficiency. (4) The reaction catalyzed by quinones lacking a long side chain follows pseudo first-order kinetics, while the reaction with quinones carrying a long side chain is of apparently higher order. (5) The rate with ubiquinone-1 is increasing pH, while with ubiquinone-9 it is decreasing. (6) The reaction mediated by short-chain quinones seems to be satuarated at lower dithionite concentration. We conclude that isoprenoid quinones are able to translocate electrons and protons in lipid membranes, and that the side chain has a strong impact on the mechanism. This and the relevance of the model reaction for electron and proton transport in photosynthesis and respiration is discussed.", "contents": "Vectorial redox reactions of physiological quinones. I. Requirement of a minimum length of the isoprenoid side chain. Physiological quinones carrying isoprenoid side chains have been compared with homologues lacking the side chain, for their ability to carry electrons and protons from dithionite to ferricyanide, trapped in liposomes. Six differential observations were made: (1) Plastoquinone and ubiquinones, with a side chain of more than two isoprene units, are by far better mediators than their short-chain homologues. Also other benzoquinones lacking a long side chain are poor catalysts, except dimethyl-methylenedioxy-p-benzoquinone, a highly autooxidizable compound. Tocopherol is a good catalyst. (2) Vitamin K-1 and K-2 are poor mediators compared to vitamin K-3. (3) The reaction catalyzed by quinones carrying long isoprenoid side chains has an about three-fold higher activation energy, irrespective of the catalytic efficiency. (4) The reaction catalyzed by quinones lacking a long side chain follows pseudo first-order kinetics, while the reaction with quinones carrying a long side chain is of apparently higher order. (5) The rate with ubiquinone-1 is increasing pH, while with ubiquinone-9 it is decreasing. (6) The reaction mediated by short-chain quinones seems to be satuarated at lower dithionite concentration. We conclude that isoprenoid quinones are able to translocate electrons and protons in lipid membranes, and that the side chain has a strong impact on the mechanism. This and the relevance of the model reaction for electron and proton transport in photosynthesis and respiration is discussed."}
{"id": "PMID:486436", "title": "Vectorial redox reactions of physiological quinones. II. A study of transient semiquinone formation.", "content": "Transient absorption changes during reduction of quinone in liposomes by external dithionite, in the absence and presence of initially trapped ferricyanide, were matched with absorption spectra of semiquinone and quinone in the blue region. Plastoquinone, ubiquinone-9 and phylloquinone, each having an isoprenoid side chain were compared with trimethyl-p-benzoquinone, ubiquinone-9 and menadione, which lack a long side chain. Semiquinone transients could only be observed by our spectroscopic technique during reduction of quinones lacking the chain. If Triton X-100 was added to the liposomes preparation semiquinone transients were also observed with the isoprenoid quinones. This result is consistent with the view that isoprenoid quinones build domains in the membranes, in which the life time of the semiquinone might be decreased by fast disproportionation, and to which dithionite has limited access.", "contents": "Vectorial redox reactions of physiological quinones. II. A study of transient semiquinone formation. Transient absorption changes during reduction of quinone in liposomes by external dithionite, in the absence and presence of initially trapped ferricyanide, were matched with absorption spectra of semiquinone and quinone in the blue region. Plastoquinone, ubiquinone-9 and phylloquinone, each having an isoprenoid side chain were compared with trimethyl-p-benzoquinone, ubiquinone-9 and menadione, which lack a long side chain. Semiquinone transients could only be observed by our spectroscopic technique during reduction of quinones lacking the chain. If Triton X-100 was added to the liposomes preparation semiquinone transients were also observed with the isoprenoid quinones. This result is consistent with the view that isoprenoid quinones build domains in the membranes, in which the life time of the semiquinone might be decreased by fast disproportionation, and to which dithionite has limited access."}
{"id": "PMID:486437", "title": "Kinetics of photoconversion of protochlorophyllide 649 to chlorophyllide 676 at low temperature in etiolated cotyledons of Pharbitis nil.", "content": "The kinetics of the photoconversion of protochlorophyllide 649 to chlorophyllide 676 were studied spectrophotometrically over the temperature range of -15 -- -80 degrees C under light-saturating conditions in etiolated cotyledons of Pharbitis nil. Photoconversion obeyed the sum of two first-order kinetics over this low temperature range. Activation energies obtained from the rate constants were about 5000 cal; this suggests that these two processes may be physical processes not chemical reactions. The results indicate that photoconversion involves two main steps. One is the step dependent on both light intensity and temperature that has been well studied. The other, which is concerned in this study, is the step dependent on temperature only, which may be the requisite for photoconversion. This latter step seems to be related to the binding mode of protochlorophyllide to a holochrome protein or to conformational changes in the protochlorophyllide-holochrome.", "contents": "Kinetics of photoconversion of protochlorophyllide 649 to chlorophyllide 676 at low temperature in etiolated cotyledons of Pharbitis nil. The kinetics of the photoconversion of protochlorophyllide 649 to chlorophyllide 676 were studied spectrophotometrically over the temperature range of -15 -- -80 degrees C under light-saturating conditions in etiolated cotyledons of Pharbitis nil. Photoconversion obeyed the sum of two first-order kinetics over this low temperature range. Activation energies obtained from the rate constants were about 5000 cal; this suggests that these two processes may be physical processes not chemical reactions. The results indicate that photoconversion involves two main steps. One is the step dependent on both light intensity and temperature that has been well studied. The other, which is concerned in this study, is the step dependent on temperature only, which may be the requisite for photoconversion. This latter step seems to be related to the binding mode of protochlorophyllide to a holochrome protein or to conformational changes in the protochlorophyllide-holochrome."}
{"id": "PMID:486438", "title": "A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts.", "content": "A detailed study of the photo-induced decline in chlorophyll a fluorescence intensity (Kautsky phenomenon) in coupled isolated chloroplasts from a high level (P) to a low stationary level (S) is presented. 1. A linear relationship between P leads to S quenching and intrathylakoid H+ concentration was found. When the light-induced proton gradient was abolished by uncoupling, the fluorescence emission at room temperature was lowered proportionally to increased H+ concentration in the medium. 2. Fluorescence spectra at -196 degrees C of samples frozen at the P and S states showed no significant differences in the Photosystem I/Photosystem II ratio of fluorescence emission. Furthermore, freezing to -196 degrees C reversed the P leads to S quenching. This indicates that the P leads to S quenching is not related to an increase of spillover of excitation energy from Photosystem II to Photosystem I. 3. When Mg2+ was added to thylakoids suspended in a medium free of divalent cations, the inhibition of spillover required lower Mg2+ concentrations (half saturation at 0.6 mM). Increased proton concentration in the medium also inhibited spillover. 4. The results are interpreted in terms of two sites of Mg2+ and H+ effects on excitation deactivation in Photosystem II. One site is located on the outer face of the thylakoid membrane; action of both Mg2+ and H+ at this side diminishes spillover. The second site is located on the inner face of the membrane; as Mg2+ is displaced there by protons, a non-photochemical quenching of Photosystem II fluorescence is induced, which is manifested by the P leads to S decline.", "contents": "A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. A detailed study of the photo-induced decline in chlorophyll a fluorescence intensity (Kautsky phenomenon) in coupled isolated chloroplasts from a high level (P) to a low stationary level (S) is presented. 1. A linear relationship between P leads to S quenching and intrathylakoid H+ concentration was found. When the light-induced proton gradient was abolished by uncoupling, the fluorescence emission at room temperature was lowered proportionally to increased H+ concentration in the medium. 2. Fluorescence spectra at -196 degrees C of samples frozen at the P and S states showed no significant differences in the Photosystem I/Photosystem II ratio of fluorescence emission. Furthermore, freezing to -196 degrees C reversed the P leads to S quenching. This indicates that the P leads to S quenching is not related to an increase of spillover of excitation energy from Photosystem II to Photosystem I. 3. When Mg2+ was added to thylakoids suspended in a medium free of divalent cations, the inhibition of spillover required lower Mg2+ concentrations (half saturation at 0.6 mM). Increased proton concentration in the medium also inhibited spillover. 4. The results are interpreted in terms of two sites of Mg2+ and H+ effects on excitation deactivation in Photosystem II. One site is located on the outer face of the thylakoid membrane; action of both Mg2+ and H+ at this side diminishes spillover. The second site is located on the inner face of the membrane; as Mg2+ is displaced there by protons, a non-photochemical quenching of Photosystem II fluorescence is induced, which is manifested by the P leads to S decline."}
{"id": "PMID:486439", "title": "Photochemical reaction of 9-cis-retro-gamma-rhodopsin at low temperatures.", "content": "9-cis-Retro-gamma-rhodopsin (lambda max = 420 nm) was prepared from 9-cis-retro-gamma-retinal and cattle opsin. After cooling to liquid nitrogen temperature (77 K), the pigment was irradiated with light at 380 nm. The spectrum shifted to the longer wavelengths, owing to formation of a batho product. This fact indicates that the conjugated double bond system from C-5 to C-8 of the chromophoric retinal in rhodopsin was not necessary for formation of bathorhodopsin. Reirradiation of the batho product with light at wavelengths longer than 520 nm yielded a mixture composed of presumably 9- or 11-cis forms of retro-gamma-rhodopsin. These three isomers are interconvertible by light at liquid nitrogen temperature. Thus the retro-gamma-rhodopsin system is similar in photochemical reaction at 77 K to cattle rhodopsin system. Each system has its own batho product. Based on these results, it was infered that the formation of batho-rhodopsin is due to photoisomerization of the chromophoric retinal of rhodopsin and is not due to translocation of a proton on the ring or on the side chain from C-6 to C-8 of the chromophoric retinal to the Schiff-base nitrogen.", "contents": "Photochemical reaction of 9-cis-retro-gamma-rhodopsin at low temperatures. 9-cis-Retro-gamma-rhodopsin (lambda max = 420 nm) was prepared from 9-cis-retro-gamma-retinal and cattle opsin. After cooling to liquid nitrogen temperature (77 K), the pigment was irradiated with light at 380 nm. The spectrum shifted to the longer wavelengths, owing to formation of a batho product. This fact indicates that the conjugated double bond system from C-5 to C-8 of the chromophoric retinal in rhodopsin was not necessary for formation of bathorhodopsin. Reirradiation of the batho product with light at wavelengths longer than 520 nm yielded a mixture composed of presumably 9- or 11-cis forms of retro-gamma-rhodopsin. These three isomers are interconvertible by light at liquid nitrogen temperature. Thus the retro-gamma-rhodopsin system is similar in photochemical reaction at 77 K to cattle rhodopsin system. Each system has its own batho product. Based on these results, it was infered that the formation of batho-rhodopsin is due to photoisomerization of the chromophoric retinal of rhodopsin and is not due to translocation of a proton on the ring or on the side chain from C-6 to C-8 of the chromophoric retinal to the Schiff-base nitrogen."}
{"id": "PMID:486441", "title": "Interaction of purple membrane with solvents. I. Applicability of solubility parameter mapping.", "content": "We carried out spectral studies on the interaction between purple membrane fragments (isolated from Halobacterium halobium) and a series of different solvents, classified quantitatively according to their solubility parameters delta d, delta p, delta h. These represent the contribution of dispersion forces, polar forces, and hydrogen bonding, respectively, to the cohesive energy density of the solvent. Purple membrane fragments, kept in the dark, were suspended in each of the solvents as well as in binary mixtures of solvents, and the spectrum of the resulting suspension was recorded in the wavelength region 250-700 nm. The interaction of each solvent with the membrane fragments can be represented by a point on either a ternary diagram, where each of the three axes represents one of the solubility parameters, or a binary diagram, where one of the two axes is a combination of two of the solubility parameters (delta v = square root of delta d2 + delta p2 or delta a = square root of delta p2 + delta h2). In the former type of solvent map the contribution of each of the parameters is distinct but only their relative contributions are expressed. In the latter the absolute values of delta i are considered. In each of these modes of presentation an inner closed region is observed. The solvents inside its borders interact with bacteriorhodopsin with a resultant spectral change. Mixtures of solvents fit the maps according to their calculated delta values. Thus, a mixture of an apolar solvent with a highly polar solvent interacts with bacteriorhodopsin, even though each of these solvents alone does not.", "contents": "Interaction of purple membrane with solvents. I. Applicability of solubility parameter mapping. We carried out spectral studies on the interaction between purple membrane fragments (isolated from Halobacterium halobium) and a series of different solvents, classified quantitatively according to their solubility parameters delta d, delta p, delta h. These represent the contribution of dispersion forces, polar forces, and hydrogen bonding, respectively, to the cohesive energy density of the solvent. Purple membrane fragments, kept in the dark, were suspended in each of the solvents as well as in binary mixtures of solvents, and the spectrum of the resulting suspension was recorded in the wavelength region 250-700 nm. The interaction of each solvent with the membrane fragments can be represented by a point on either a ternary diagram, where each of the three axes represents one of the solubility parameters, or a binary diagram, where one of the two axes is a combination of two of the solubility parameters (delta v = square root of delta d2 + delta p2 or delta a = square root of delta p2 + delta h2). In the former type of solvent map the contribution of each of the parameters is distinct but only their relative contributions are expressed. In the latter the absolute values of delta i are considered. In each of these modes of presentation an inner closed region is observed. The solvents inside its borders interact with bacteriorhodopsin with a resultant spectral change. Mixtures of solvents fit the maps according to their calculated delta values. Thus, a mixture of an apolar solvent with a highly polar solvent interacts with bacteriorhodopsin, even though each of these solvents alone does not."}
{"id": "PMID:486442", "title": "Interaction of purple membrane with solvents. II. Mode of interaction.", "content": "Using the solubility parameter mapping technique (Eisenbach, M., Caplan, S.R. and Tanny, G (1979) Biochim. Biophys. Acta 554, 269-280) we studied spectroscopically the mode of interaction between the purple membrane of Halobacterium halobium and pure organic solvents or solvent mixtures. Although the interacting solvents formed a well-defined closed region in the interaction maps, mapping the modes of interaction did not reveal a closed region for each spectrally classifiable type. A suggested interpretation for this is that interaction with the purple membrane chromophore requires that a solvent (or solvent mixture) possess apolar groups in order to obtain access to the chromophore, together with a polar character and hydrogen-bonding capacity. The mode of interaction, however, is dependent on the specificity of the reactive group of the solvent for retinal, and this has nothing to do with membrane properties. We also examined the influence of the duration of the interaction and of illumination. Some solvents appeared to react more sluggishly than others, but no generalization in terms of the solubility parameter mapping was found, probably because the map describes thermodynamic rather than kinetic phenomena. The only effect of illumination was to enhance the reaction of some of these solvents. It did not change the solubility parameters of purple membrane.", "contents": "Interaction of purple membrane with solvents. II. Mode of interaction. Using the solubility parameter mapping technique (Eisenbach, M., Caplan, S.R. and Tanny, G (1979) Biochim. Biophys. Acta 554, 269-280) we studied spectroscopically the mode of interaction between the purple membrane of Halobacterium halobium and pure organic solvents or solvent mixtures. Although the interacting solvents formed a well-defined closed region in the interaction maps, mapping the modes of interaction did not reveal a closed region for each spectrally classifiable type. A suggested interpretation for this is that interaction with the purple membrane chromophore requires that a solvent (or solvent mixture) possess apolar groups in order to obtain access to the chromophore, together with a polar character and hydrogen-bonding capacity. The mode of interaction, however, is dependent on the specificity of the reactive group of the solvent for retinal, and this has nothing to do with membrane properties. We also examined the influence of the duration of the interaction and of illumination. Some solvents appeared to react more sluggishly than others, but no generalization in terms of the solubility parameter mapping was found, probably because the map describes thermodynamic rather than kinetic phenomena. The only effect of illumination was to enhance the reaction of some of these solvents. It did not change the solubility parameters of purple membrane."}
{"id": "PMID:486443", "title": "Isolation of human platelet and red blood cell plasma membrane proteins by preparative detergent electrophoresis.", "content": "High resolution polyacrylamide gel electrophoretic techniques have been applied to the preparative isolation and analysis of plasma membrane proteins and glycoproteins from human platelets and red blood cells. The techniques presented allow relatively simple, direct, rapid and quantitative purification of a broad molecular weight range of membrane proteins, by means of continuous elution preparative gel electrophoresis of protein solubilized with sodium dodecyl sulfate. Spectrophotometric and fluorophotometric (fluorescamine) profiling, and high resolution gel electrophoretic analysis (SDS-acrylamide gradient slab gels, and gel electrofocusing) of eluted protein species indicate that purified membrane proteins of a broad molecular weight range may be obtained in a one step procedure, and in quantities and concentrations sufficient for further analytical or experimental procedures.", "contents": "Isolation of human platelet and red blood cell plasma membrane proteins by preparative detergent electrophoresis. High resolution polyacrylamide gel electrophoretic techniques have been applied to the preparative isolation and analysis of plasma membrane proteins and glycoproteins from human platelets and red blood cells. The techniques presented allow relatively simple, direct, rapid and quantitative purification of a broad molecular weight range of membrane proteins, by means of continuous elution preparative gel electrophoresis of protein solubilized with sodium dodecyl sulfate. Spectrophotometric and fluorophotometric (fluorescamine) profiling, and high resolution gel electrophoretic analysis (SDS-acrylamide gradient slab gels, and gel electrofocusing) of eluted protein species indicate that purified membrane proteins of a broad molecular weight range may be obtained in a one step procedure, and in quantities and concentrations sufficient for further analytical or experimental procedures."}
{"id": "PMID:486444", "title": "Plasma membrane from Candida tropicalis grown on glucose or hexadecane. II. Biochemical properties and substrate-induced alterations.", "content": "Isolated plasma membranes from the yeast Candida tropicalis grown on two different carbon sources (glucose or hexadecane), had similar contents of protein (60% of total dry weight), lipid (21-24%) and carbohydrates (16-21%). Sodium dodecyl sulphate gel electrophoresis of the membrane proteins revealed 17 and 19 protein bands, respectively, for glucose and hexadecane grown cells. There were marked differences in RF values and relative peak heights between the two gels. Sterols and free fatty acids were the major components of the plasma membrane lipids. Phospholipid content was less than 2% of total plasma membrane lipids. Membrane microviscosity, as determined by fluorescence polarization, was very high (16.6 P). Fatty acid determination of membrane lipids by gas chromatography showed a significant increase of C16 fatty acids in plasma membranes of cells grown on hexadecane. Reduced-oxidized difference spectra demonstrated the presence of a b-type cytochrome in both Saccharomyces cerevisiae and C. tropicalis plasma membranes. Its concentration in C. tropicalis plasma membranes was three-fold greater in cells grown on hexadecane than in glucose grown cells.", "contents": "Plasma membrane from Candida tropicalis grown on glucose or hexadecane. II. Biochemical properties and substrate-induced alterations. Isolated plasma membranes from the yeast Candida tropicalis grown on two different carbon sources (glucose or hexadecane), had similar contents of protein (60% of total dry weight), lipid (21-24%) and carbohydrates (16-21%). Sodium dodecyl sulphate gel electrophoresis of the membrane proteins revealed 17 and 19 protein bands, respectively, for glucose and hexadecane grown cells. There were marked differences in RF values and relative peak heights between the two gels. Sterols and free fatty acids were the major components of the plasma membrane lipids. Phospholipid content was less than 2% of total plasma membrane lipids. Membrane microviscosity, as determined by fluorescence polarization, was very high (16.6 P). Fatty acid determination of membrane lipids by gas chromatography showed a significant increase of C16 fatty acids in plasma membranes of cells grown on hexadecane. Reduced-oxidized difference spectra demonstrated the presence of a b-type cytochrome in both Saccharomyces cerevisiae and C. tropicalis plasma membranes. Its concentration in C. tropicalis plasma membranes was three-fold greater in cells grown on hexadecane than in glucose grown cells."}
{"id": "PMID:486446", "title": "Lipid packing and transbilayer asymmetries of mixed lipid vesicles.", "content": "Predictions, based on a previously developed theory, of the radii and asymmetric lipid distribution of mixed phosphatidylcholine/lysophosphatidylcholine and phosphatidylcholine/cholesterol vesicles of variable composition are presented. The results compare well with available experimental data, except for cis-unsaturated phosphatidylcholine/cholesterol vesicles at high concentrations of cholesterol. It is concluded that specific lipid-lipid interactions need not be invoked for saturated and trans-unsaturated phosphatidylcholine mixed with lysophosphatidylcholine or cholesterol. A discussion of the effect of packing stresses on induced flip-flop and non-spherical vesicles is also presented.", "contents": "Lipid packing and transbilayer asymmetries of mixed lipid vesicles. Predictions, based on a previously developed theory, of the radii and asymmetric lipid distribution of mixed phosphatidylcholine/lysophosphatidylcholine and phosphatidylcholine/cholesterol vesicles of variable composition are presented. The results compare well with available experimental data, except for cis-unsaturated phosphatidylcholine/cholesterol vesicles at high concentrations of cholesterol. It is concluded that specific lipid-lipid interactions need not be invoked for saturated and trans-unsaturated phosphatidylcholine mixed with lysophosphatidylcholine or cholesterol. A discussion of the effect of packing stresses on induced flip-flop and non-spherical vesicles is also presented."}
{"id": "PMID:486447", "title": "Interaction of rabbit muscle aldolase with phospholipid liposomes.", "content": "The interaction between rabbit muscle fructose diphosphate aldolase and phospholipid model membranes (liposomes) was studied by measurement of the tryptophan fluorescence of the enzyme. Interaction with liposomes decreases intrinsic fluorescence intensity of the enzyme and shifts the emission wavelength maximum to higher values. The effects appear to be strongly dependent on the nature of the phospholipid polar group and on ionic strength. Also, a reversible modification of specific activity of aldolase upon interaction with liposomes was found. It is postulated that aldolase binds to liposomes mainly by electrostatic interactions and that the binding causes a change in the conformation of the enzyme.", "contents": "Interaction of rabbit muscle aldolase with phospholipid liposomes. The interaction between rabbit muscle fructose diphosphate aldolase and phospholipid model membranes (liposomes) was studied by measurement of the tryptophan fluorescence of the enzyme. Interaction with liposomes decreases intrinsic fluorescence intensity of the enzyme and shifts the emission wavelength maximum to higher values. The effects appear to be strongly dependent on the nature of the phospholipid polar group and on ionic strength. Also, a reversible modification of specific activity of aldolase upon interaction with liposomes was found. It is postulated that aldolase binds to liposomes mainly by electrostatic interactions and that the binding causes a change in the conformation of the enzyme."}
{"id": "PMID:486448", "title": "Asymmetric labeling of amino lipids in liposomes.", "content": "Fluorescamine and trinitrobenzenesulfonate were used as chemical probes to differentially label amino phospholipids in liposomes. At low concentrations, fluorescamine reacts primarily with amino lipids on the external half of the bilayer. Further increase in fluorescamine concentration resulted in a linear increase of labeling indicating penetration and reaction with the internal half of the bilayer. Because of the pH requirements of the fluorescamine reaction, internal labeling was eliminated with a H+ gradient: inside acidic/outside alkaline. Differential labeling was also achieved with trinitrobenzenesulfonate, which is normally not permeable but which can be transported by valinomycin-K+ complex and react with internal amines. Thus, either half of the bilayer can be labeled with the same or different reagents. When liposomes were double-labeled, the fluorescence of fluorescamine was quenched by the trinitrobenzenesulfonate label. This quenching was reversed by solubilizing the liposomes with acidic ethanol. No quenching occurred when fluorescamine-labeled liposomes were mixed with trinitrobenzenesulfonate-reacted liposomes (or trinitrophenylated methylamine) suggesting close proximity of two labels is required for quenching. Conditions which promoted vesicular fusion promptly produced quenching. These differential labeling procedures can be usefully applied to quantitate aminolipids on internal and external vesicular surface, monitor vesicular fusion, and assess liposomal structure.", "contents": "Asymmetric labeling of amino lipids in liposomes. Fluorescamine and trinitrobenzenesulfonate were used as chemical probes to differentially label amino phospholipids in liposomes. At low concentrations, fluorescamine reacts primarily with amino lipids on the external half of the bilayer. Further increase in fluorescamine concentration resulted in a linear increase of labeling indicating penetration and reaction with the internal half of the bilayer. Because of the pH requirements of the fluorescamine reaction, internal labeling was eliminated with a H+ gradient: inside acidic/outside alkaline. Differential labeling was also achieved with trinitrobenzenesulfonate, which is normally not permeable but which can be transported by valinomycin-K+ complex and react with internal amines. Thus, either half of the bilayer can be labeled with the same or different reagents. When liposomes were double-labeled, the fluorescence of fluorescamine was quenched by the trinitrobenzenesulfonate label. This quenching was reversed by solubilizing the liposomes with acidic ethanol. No quenching occurred when fluorescamine-labeled liposomes were mixed with trinitrobenzenesulfonate-reacted liposomes (or trinitrophenylated methylamine) suggesting close proximity of two labels is required for quenching. Conditions which promoted vesicular fusion promptly produced quenching. These differential labeling procedures can be usefully applied to quantitate aminolipids on internal and external vesicular surface, monitor vesicular fusion, and assess liposomal structure."}
{"id": "PMID:486449", "title": "Influence of enzymatic phospholipid cleavage on the permeability of the erythrocyte membrane. I. Transport of non-electrolytes via the lipid domain.", "content": "In order to further elucidate the influence of membrane lipids on transport via the lipid domain of the erythrocyte membrane, simple non-electrolyte diffusion was investigated by tracer flux measurements in whole cells after cleavage of up to 65% of phosphatidylcholine or sphingomyelin by phospholipase A2 from Naja naja, or by sphingomyelinase. A new type of labelled model non-electrolyte was used in this study, readily available by reacting a non-labelled thiol with a labelled alkylating SH-reagent. In spite of the marked enzymatic alterations of the membrane, which lead to the occurrence of large quantities of lysophosphatidylcholine and long chain fatty acids, or of ceramide, the permeability of the lipid domain remained unaffected. This finding is very surprising, since the physical properties of the lipid phase (microviscosity, structure of the membrane interface) are likely to be perturbed in the enzyme-treated membranes. Sphingomyelinase-treated cells undergo stomatocytic shape changes followed by deep invaginations of the membrane and finally endocytosis, while phospholipase A2-treated cells essentially maintain their normal shape.", "contents": "Influence of enzymatic phospholipid cleavage on the permeability of the erythrocyte membrane. I. Transport of non-electrolytes via the lipid domain. In order to further elucidate the influence of membrane lipids on transport via the lipid domain of the erythrocyte membrane, simple non-electrolyte diffusion was investigated by tracer flux measurements in whole cells after cleavage of up to 65% of phosphatidylcholine or sphingomyelin by phospholipase A2 from Naja naja, or by sphingomyelinase. A new type of labelled model non-electrolyte was used in this study, readily available by reacting a non-labelled thiol with a labelled alkylating SH-reagent. In spite of the marked enzymatic alterations of the membrane, which lead to the occurrence of large quantities of lysophosphatidylcholine and long chain fatty acids, or of ceramide, the permeability of the lipid domain remained unaffected. This finding is very surprising, since the physical properties of the lipid phase (microviscosity, structure of the membrane interface) are likely to be perturbed in the enzyme-treated membranes. Sphingomyelinase-treated cells undergo stomatocytic shape changes followed by deep invaginations of the membrane and finally endocytosis, while phospholipase A2-treated cells essentially maintain their normal shape."}
{"id": "PMID:486450", "title": "Influence of enzymatic phospholipid cleavage on the permeability of the erythrocyte membrane. II. Protein-mediated transfer of monosaccharides and anions.", "content": "In order to investigate the influence of membrane lipids on transport via the protein domain of the erythrocyte membrane, a number of facilitated diffusion processes was studied by tracer flux techniques in whole cells after cleavage of up to 65% of the phosphatidylcholine or the sphingomyelin by phospholipase A2 from Naja naja or bee venom, or by sphingomyelinase, respectively. The mediated fluxes of L-arabinose, which is transported by the glucose carrier, and of L-lactate, which uses a specific monocarboxylate carrier, were markedly inhibited by cleavage of either phosphatidylcholine or sphingomyelin. These phospholipid dependencies are in line with earlier data on cholesterol dependencies (Deuticke, B. (1977) Rev. Physiol. Biochem. Pharmacol. 78, 1-97). They can only in part be explained by changes of membrane fluidity. More specific interactions of the degradation products with the carrier proteins seem also to play a role. Sulfate and oxalate transfer, which proceed via the inorganic anion-exchange system, are essentially unaffected by cleavage of phosphatidylcholine and less sensitive to sphingomyelin cleavage than the two other processes. This also agrees with earlier data on cholesterol independency of sulfate transfer. The inorganic anion-exchange protein thus seems to be less dependent on the surrounding lipids in its conformation and its mode of action than the two other carriers.", "contents": "Influence of enzymatic phospholipid cleavage on the permeability of the erythrocyte membrane. II. Protein-mediated transfer of monosaccharides and anions. In order to investigate the influence of membrane lipids on transport via the protein domain of the erythrocyte membrane, a number of facilitated diffusion processes was studied by tracer flux techniques in whole cells after cleavage of up to 65% of the phosphatidylcholine or the sphingomyelin by phospholipase A2 from Naja naja or bee venom, or by sphingomyelinase, respectively. The mediated fluxes of L-arabinose, which is transported by the glucose carrier, and of L-lactate, which uses a specific monocarboxylate carrier, were markedly inhibited by cleavage of either phosphatidylcholine or sphingomyelin. These phospholipid dependencies are in line with earlier data on cholesterol dependencies (Deuticke, B. (1977) Rev. Physiol. Biochem. Pharmacol. 78, 1-97). They can only in part be explained by changes of membrane fluidity. More specific interactions of the degradation products with the carrier proteins seem also to play a role. Sulfate and oxalate transfer, which proceed via the inorganic anion-exchange system, are essentially unaffected by cleavage of phosphatidylcholine and less sensitive to sphingomyelin cleavage than the two other processes. This also agrees with earlier data on cholesterol independency of sulfate transfer. The inorganic anion-exchange protein thus seems to be less dependent on the surrounding lipids in its conformation and its mode of action than the two other carriers."}
{"id": "PMID:486451", "title": "Ion transport in the simplest single file pore.", "content": "A kinetic scheme is developed to describe single-file transport through pores containing up to two ions which may be of different species. The solution for the fluxes in terms of rate constants for entry, exit, and transfer is derived without specific assumptions about symmetry or the voltage and activity dependence of the constants. For a symmetrical pore the relation between the slope conductance at zero applied potential and ion activity can have two distinct regions in which the conductance increases linearly. Zero current or reversal potentials depend on the absolute values of the activities as well as their ratios. The use of this theory to describe the cation fluxes through the pores formed by gramicidin A will be considered in a subsequent paper. Here the model is discussed for a number of more specific assumptions, most extensively the following combination: (1) while entry to a pore is less likely when the pore is already occupied at the opposite end, this entry is still rapid; (2) exit is much more rapid when the pore is occupied by two ions; and (3) transfer from one end to the other of a singly occupied pore is rapid. With these assumptions and for a range of concentrations over which the fluxes are proportional to ion activities, the model predicts a flux ratio exponent nearly equal to 2, blocking by impermeant ions, rectification due to blocking particles on one side only, relief of block by increase in the permeant ion concentration on the opposite side, and anomalous variations of the conductance and zero current potential with mole ratio when the total concentration of the two permeants is held constant.", "contents": "Ion transport in the simplest single file pore. A kinetic scheme is developed to describe single-file transport through pores containing up to two ions which may be of different species. The solution for the fluxes in terms of rate constants for entry, exit, and transfer is derived without specific assumptions about symmetry or the voltage and activity dependence of the constants. For a symmetrical pore the relation between the slope conductance at zero applied potential and ion activity can have two distinct regions in which the conductance increases linearly. Zero current or reversal potentials depend on the absolute values of the activities as well as their ratios. The use of this theory to describe the cation fluxes through the pores formed by gramicidin A will be considered in a subsequent paper. Here the model is discussed for a number of more specific assumptions, most extensively the following combination: (1) while entry to a pore is less likely when the pore is already occupied at the opposite end, this entry is still rapid; (2) exit is much more rapid when the pore is occupied by two ions; and (3) transfer from one end to the other of a singly occupied pore is rapid. With these assumptions and for a range of concentrations over which the fluxes are proportional to ion activities, the model predicts a flux ratio exponent nearly equal to 2, blocking by impermeant ions, rectification due to blocking particles on one side only, relief of block by increase in the permeant ion concentration on the opposite side, and anomalous variations of the conductance and zero current potential with mole ratio when the total concentration of the two permeants is held constant."}
{"id": "PMID:486452", "title": "Glycodeoxycholate transport in brush border membrane vesicles isolated from rat jejunum and ileum.", "content": "The transport of the bile salt, glycodeoxycholate, was studied in vesicles derived from rat jejunal and ileal brush border membranes using a rapid filtration technique. The uptake was osmotically sensitive, linearly related to membrane protein and resembled D-glucose transport. In ileal, but not jejunal, vesicles glycodeoxycholate uptake showed a transient vesicle/medium ratio greater than 1 in the presence of an initial sodium gradient. The differences between glycodeoxycholate uptake in the presence and absence of a Na+ gradient yielded a saturable transport component. Kinetic analysis revealed a Km value similar to that described previously in everted whole intestinal segments and epithelial cells isolated from the ileum. These findings support the existence of a transport system in the brush border membrane that: (1) reflects kinetics and characteristics of bile salt transport in intact intestinal preparations, and (2) catalyzes the co-transport of Na+ and bile salt across the ileal membrane in a manner analogous to D-glucose transport.", "contents": "Glycodeoxycholate transport in brush border membrane vesicles isolated from rat jejunum and ileum. The transport of the bile salt, glycodeoxycholate, was studied in vesicles derived from rat jejunal and ileal brush border membranes using a rapid filtration technique. The uptake was osmotically sensitive, linearly related to membrane protein and resembled D-glucose transport. In ileal, but not jejunal, vesicles glycodeoxycholate uptake showed a transient vesicle/medium ratio greater than 1 in the presence of an initial sodium gradient. The differences between glycodeoxycholate uptake in the presence and absence of a Na+ gradient yielded a saturable transport component. Kinetic analysis revealed a Km value similar to that described previously in everted whole intestinal segments and epithelial cells isolated from the ileum. These findings support the existence of a transport system in the brush border membrane that: (1) reflects kinetics and characteristics of bile salt transport in intact intestinal preparations, and (2) catalyzes the co-transport of Na+ and bile salt across the ileal membrane in a manner analogous to D-glucose transport."}
{"id": "PMID:486453", "title": "The 'hollow cylinder' protein of erythrocyte membranes.", "content": "The 'hollow cylinder' protein (Harris, J.R. (1968) Biochim. Biophys. Acta 150, 534-537) has been purified from human erythrocyte membranes. The molecular weight of the native protein, as determined by analytical ultracentrifugation, was found to be 747,000. By means of sodium dodecyl sulphate gel electrophoresis, the purified protein was shown to be composed of three different low molecular weight polypeptides of average molecular weight 25,000. This study provides convincing evidence that the spectrin tetramer is not responsible for the characteristic electron microscopic appearance of the hollow cylinder protein.", "contents": "The 'hollow cylinder' protein of erythrocyte membranes. The 'hollow cylinder' protein (Harris, J.R. (1968) Biochim. Biophys. Acta 150, 534-537) has been purified from human erythrocyte membranes. The molecular weight of the native protein, as determined by analytical ultracentrifugation, was found to be 747,000. By means of sodium dodecyl sulphate gel electrophoresis, the purified protein was shown to be composed of three different low molecular weight polypeptides of average molecular weight 25,000. This study provides convincing evidence that the spectrin tetramer is not responsible for the characteristic electron microscopic appearance of the hollow cylinder protein."}
{"id": "PMID:486455", "title": "Anion transport across the erythrocyte membrane, in situ proteolysis of band 3 protein, and cross-linking of proteolytic fragments by 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonate.", "content": "Extracellular chymotrypsin cleaves the 95 000 dalton protein that migrates in band 3 of SDS-polyacrylamide gel electropherograms of the erythrocyte membrane into fragments of 60 000 and 35 000 daltons, but not further. Minor components of band 3 that remain at the original 95 000 dalton location may be eluted from the membrane by 0.1 N NaOH, indicating that, in contrast to the major component and the chymotryptic fragments, they are not integral membrane constituents. Incubation at neutral pH of chymotrypsinized erythrocytes with the bifunctional anion transport inhibitor 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid results in covalent binding of that inhibitor primarily to the 60 000 dalton fragment and some cross-linking of the 60 000 dalton fragment with the 35 000 dalton fragment. Increasing the pH to 9.5 leads to a cross-linking of virtually all of the pairs of chymotryptic fragments and thus to a reconstitution of band 3 with its typical diffuse appearance in the 95 000 dalton region of the SDS-polyacrylamide gels. This indicates that (1) each integral 95 000 dalton protein molecule is capable of binding at least one 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid molecule; (2) the 35 000 dalton fragment, though it is only weakly stained with Coomassie blue, is present in an amount that is equimolar with that of the 60 000 dalton fragment. Since the number of 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid binding sites on the protein in band 3/cell is known to be close to the number of band 3 molecules/cell, it is suggested that the cross-linking takes place at a region of the band 3 molecule that is involved in the control of anion transport, Like chymotrypsin, papain digests the band 3 protein from the outer membrane surface. Unlike chymotrypsin, however, papain digestion results in an inhibition of anion exchange. Papain produces a major fragment of 60 000 daltons that differs from the major chymotryptic fragment by at most six amino acid residues. The only detectable difference between the noninhibitory action of chymotrypsin and the inhibitory action of papain on the band 3 protein is that papain is capable of partially digesting the 35000 dalton fragment. No reconstitution of band 3 by cross-linking of the fragments with 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid can be achieved. Since the 35 000 dalton fragment reacts with one of the two reactive groups of 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid and is also susceptible to digestion by the inhibitory papain, we suggest that a portion of this peptide participates, together with a portion of the 60 000 dalton fragment, in the control anion transport.", "contents": "Anion transport across the erythrocyte membrane, in situ proteolysis of band 3 protein, and cross-linking of proteolytic fragments by 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonate. Extracellular chymotrypsin cleaves the 95 000 dalton protein that migrates in band 3 of SDS-polyacrylamide gel electropherograms of the erythrocyte membrane into fragments of 60 000 and 35 000 daltons, but not further. Minor components of band 3 that remain at the original 95 000 dalton location may be eluted from the membrane by 0.1 N NaOH, indicating that, in contrast to the major component and the chymotryptic fragments, they are not integral membrane constituents. Incubation at neutral pH of chymotrypsinized erythrocytes with the bifunctional anion transport inhibitor 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid results in covalent binding of that inhibitor primarily to the 60 000 dalton fragment and some cross-linking of the 60 000 dalton fragment with the 35 000 dalton fragment. Increasing the pH to 9.5 leads to a cross-linking of virtually all of the pairs of chymotryptic fragments and thus to a reconstitution of band 3 with its typical diffuse appearance in the 95 000 dalton region of the SDS-polyacrylamide gels. This indicates that (1) each integral 95 000 dalton protein molecule is capable of binding at least one 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid molecule; (2) the 35 000 dalton fragment, though it is only weakly stained with Coomassie blue, is present in an amount that is equimolar with that of the 60 000 dalton fragment. Since the number of 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid binding sites on the protein in band 3/cell is known to be close to the number of band 3 molecules/cell, it is suggested that the cross-linking takes place at a region of the band 3 molecule that is involved in the control of anion transport, Like chymotrypsin, papain digests the band 3 protein from the outer membrane surface. Unlike chymotrypsin, however, papain digestion results in an inhibition of anion exchange. Papain produces a major fragment of 60 000 daltons that differs from the major chymotryptic fragment by at most six amino acid residues. The only detectable difference between the noninhibitory action of chymotrypsin and the inhibitory action of papain on the band 3 protein is that papain is capable of partially digesting the 35000 dalton fragment. No reconstitution of band 3 by cross-linking of the fragments with 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid can be achieved. Since the 35 000 dalton fragment reacts with one of the two reactive groups of 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonic acid and is also susceptible to digestion by the inhibitory papain, we suggest that a portion of this peptide participates, together with a portion of the 60 000 dalton fragment, in the control anion transport."}
{"id": "PMID:486456", "title": "Lectin-mediated agglutination of murine lymphoma cells. Cell surface deformability and reversibility of agglutination by saccharides.", "content": "Agglutination of S49 mouse lymphoma cells by Ricinus communis I agglutinin can be reversed by the competing haptenic saccharide, lactose, soon after agglutination, but after further incubation in the absence of lectin the agglutination reaction could not be reversed by lactose and the cells remained as multicell aggregates. The irreversibility of S49 cell agglutination was time, temperature and lectin concentration dependent and its onset correlated with ultrastructurally observed deformation of adjacent cell surfaces and an increase in the proportion of adjacent cell surface areas in close apposition within multicell aggregates. Pretreatment of S49 cells with cytochalasin B or cytochalasin B plus vinblastine enhanced R. communis I agglutinin-mediated agglutination, while vinblastine alone and fluoride plus azide had essentially no effect. When drug-treated cells were agglutinated and then incubated in lectin-free drug-containing media for various times prior to lactose addition, the drug effects were more pronounced. Cytochalasin B alone or with vinblastine inhibited lactose reversal of S49 cell agglutination compared to the drug-free controls, while fluoride plus azide enhanced hapten reversibility. Electron microscopic analysis revealed that the onset of agglutination irreversibility correlated with cell surface deformation in the drug-treated cells. Cell aggregates that were more readily reversible by lactose (fluoride plus azide) were unchanged or less deformed, while S49 aggregates treated with cytochalasin B plus vinblastine were more deformed compared to controls without drugs. These experiments suggest a role for cell surface deformability as an important secondary effect during lectin-mediated cell agglutination of S49 lymphoma cells.", "contents": "Lectin-mediated agglutination of murine lymphoma cells. Cell surface deformability and reversibility of agglutination by saccharides. Agglutination of S49 mouse lymphoma cells by Ricinus communis I agglutinin can be reversed by the competing haptenic saccharide, lactose, soon after agglutination, but after further incubation in the absence of lectin the agglutination reaction could not be reversed by lactose and the cells remained as multicell aggregates. The irreversibility of S49 cell agglutination was time, temperature and lectin concentration dependent and its onset correlated with ultrastructurally observed deformation of adjacent cell surfaces and an increase in the proportion of adjacent cell surface areas in close apposition within multicell aggregates. Pretreatment of S49 cells with cytochalasin B or cytochalasin B plus vinblastine enhanced R. communis I agglutinin-mediated agglutination, while vinblastine alone and fluoride plus azide had essentially no effect. When drug-treated cells were agglutinated and then incubated in lectin-free drug-containing media for various times prior to lactose addition, the drug effects were more pronounced. Cytochalasin B alone or with vinblastine inhibited lactose reversal of S49 cell agglutination compared to the drug-free controls, while fluoride plus azide enhanced hapten reversibility. Electron microscopic analysis revealed that the onset of agglutination irreversibility correlated with cell surface deformation in the drug-treated cells. Cell aggregates that were more readily reversible by lactose (fluoride plus azide) were unchanged or less deformed, while S49 aggregates treated with cytochalasin B plus vinblastine were more deformed compared to controls without drugs. These experiments suggest a role for cell surface deformability as an important secondary effect during lectin-mediated cell agglutination of S49 lymphoma cells."}
{"id": "PMID:486457", "title": "Isolation of glycophorin with deoxycholate.", "content": "In a previous communication we reported that human erythrocyte glycophorin prepared by the lithium diiodosalicylate phenol procedure contains approximately 10 mol of lithium diiodosalicylate per mol of glycophorin, and further we showed that this bound lithium diiodosalicylate is difficult to remove by detergents or organic solvents (Romans, A.Y. and Segrest, J.P. (1978) Biochim. Biophys. Acta 511, 297-301). In the present communication we report an alternative purification procedure for glycophorin in which sodium deoxycholate is substituted for lithium diiodosalicylate; the sodium deoxycholate is subsequently removed by gel filtration. Utilizing this procedure, 25-30 mg glycophorin are obtained per gram of lyophilized erythrocyte ghosts. The glycophorin prepared by the sodium deoxycholate procedure, after a single gel filtration step, contains less than 1 mol of sodium deoxycholate per mol glycophorin and is colorless compared with glycophorin prepared by the lithium diiodosalicylate procedure, which has a distint reddish-brown cast.", "contents": "Isolation of glycophorin with deoxycholate. In a previous communication we reported that human erythrocyte glycophorin prepared by the lithium diiodosalicylate phenol procedure contains approximately 10 mol of lithium diiodosalicylate per mol of glycophorin, and further we showed that this bound lithium diiodosalicylate is difficult to remove by detergents or organic solvents (Romans, A.Y. and Segrest, J.P. (1978) Biochim. Biophys. Acta 511, 297-301). In the present communication we report an alternative purification procedure for glycophorin in which sodium deoxycholate is substituted for lithium diiodosalicylate; the sodium deoxycholate is subsequently removed by gel filtration. Utilizing this procedure, 25-30 mg glycophorin are obtained per gram of lyophilized erythrocyte ghosts. The glycophorin prepared by the sodium deoxycholate procedure, after a single gel filtration step, contains less than 1 mol of sodium deoxycholate per mol glycophorin and is colorless compared with glycophorin prepared by the lithium diiodosalicylate procedure, which has a distint reddish-brown cast."}
{"id": "PMID:486458", "title": "Saturation behavior of ascites tumor cell chloride exchange in the presence of gluconate.", "content": "Steady state Cl- flux across the Ehrlich mouse ascites cell membrane was studied when gluconate replaced Cl- in the external medium. Saturation behavior was observed; K 1/2 was 23.9 mM Cl- and V was 758 micromol.g-1 dry weight.h-1. The cells lost K+, Cl- and H2O, consistent with relative impermeability to gluconate, and the Cl- efflux rate coefficient was elevated. The results indicate that a major portion of Cl- exchange occurs as a membrane transport process and suggest that the process is sensitive to intracellular Cl- levels.", "contents": "Saturation behavior of ascites tumor cell chloride exchange in the presence of gluconate. Steady state Cl- flux across the Ehrlich mouse ascites cell membrane was studied when gluconate replaced Cl- in the external medium. Saturation behavior was observed; K 1/2 was 23.9 mM Cl- and V was 758 micromol.g-1 dry weight.h-1. The cells lost K+, Cl- and H2O, consistent with relative impermeability to gluconate, and the Cl- efflux rate coefficient was elevated. The results indicate that a major portion of Cl- exchange occurs as a membrane transport process and suggest that the process is sensitive to intracellular Cl- levels."}
{"id": "PMID:486459", "title": "Electron diffraction studies of human erythrocyte membrane and its lipid extracts. Effects of hydration, temperature and hydrolysis.", "content": "The organization of lipid molecules in individual human erythrocyte ghost membranes and single bilayers of their total lipid extracts were studied by low-dose electron diffraction in a controlled environment. The highest onset temperature (Ts) at which diffraction rings corresponding to a gel state appeared, were found to be in the range of -2 to -4 degrees C for both the whole ghost membrane and bilayers of its total lipid extracts. The onsets were abolished by dehydration before separated crystallizations of cholesterol and phospholipid occurred. Ts increased as a result of free fatty acids accumulation in membranes after phospholipase A2 treatment or storage.", "contents": "Electron diffraction studies of human erythrocyte membrane and its lipid extracts. Effects of hydration, temperature and hydrolysis. The organization of lipid molecules in individual human erythrocyte ghost membranes and single bilayers of their total lipid extracts were studied by low-dose electron diffraction in a controlled environment. The highest onset temperature (Ts) at which diffraction rings corresponding to a gel state appeared, were found to be in the range of -2 to -4 degrees C for both the whole ghost membrane and bilayers of its total lipid extracts. The onsets were abolished by dehydration before separated crystallizations of cholesterol and phospholipid occurred. Ts increased as a result of free fatty acids accumulation in membranes after phospholipase A2 treatment or storage."}
{"id": "PMID:486461", "title": "Isolation of human platelet glycoproteins.", "content": "Human platelet glycoproteins were isolated from whole platelets by two methods. The first method, that of affinity chromatography on wheat germ agglutinin, is based on the known affinity of lectins for cell surface glycoproteins. When solubilized whole platelets are used as starting material for this procedure, elution with N-acetylglucosamine yields primarily a glycoprotein of Mr approximately 150 000 as estimated by sodium dodecyl sulfate-acrylamide gel electrophoresis. The second method is based on the ability of the chaotropic salt lithium diiodosalicylate to extract glycoprotein from particulate cell fractions in water-soluble form. This method yields three major glycopeptides with apparent molecular weights after sulfhydryl reduction of 145 000, 125 000, and 95 000 as estimated on 5.6% sodium dodecyl sulfate-acrylamide gels. Carboxymethylation of these preparations in the presence of sulfhydryl-reducing agent further resolves a glycoprotein of Mr approximately 165 000. Treatment of whole platelets by periodate oxidation and sodium[3H]-borohydride reduction labels the three major glycoproteins extracted by lithium diiodosalicylate and the glycoprotein of Mr approximately 150 000 isolated on wheat germ agglutinin confirming their surface orientation. However, glycoprotein with Mr approximately 165 000 resolved by carboxymethylation of the lithium diiodosalicylate extracted glycoprotein mixture was not labelled by this method, suggesting that it represents the granule protein with similar electrophoretic characteristics described by others. Phosphorylation of intact platelets with 32Pi also results in labelling of glycoproteins isolated by both methods, suggesting that these molecules traverse the bilipid layer of the platelet membrane, bearing reactive groups on both outer and cytoplasmic surfaces.", "contents": "Isolation of human platelet glycoproteins. Human platelet glycoproteins were isolated from whole platelets by two methods. The first method, that of affinity chromatography on wheat germ agglutinin, is based on the known affinity of lectins for cell surface glycoproteins. When solubilized whole platelets are used as starting material for this procedure, elution with N-acetylglucosamine yields primarily a glycoprotein of Mr approximately 150 000 as estimated by sodium dodecyl sulfate-acrylamide gel electrophoresis. The second method is based on the ability of the chaotropic salt lithium diiodosalicylate to extract glycoprotein from particulate cell fractions in water-soluble form. This method yields three major glycopeptides with apparent molecular weights after sulfhydryl reduction of 145 000, 125 000, and 95 000 as estimated on 5.6% sodium dodecyl sulfate-acrylamide gels. Carboxymethylation of these preparations in the presence of sulfhydryl-reducing agent further resolves a glycoprotein of Mr approximately 165 000. Treatment of whole platelets by periodate oxidation and sodium[3H]-borohydride reduction labels the three major glycoproteins extracted by lithium diiodosalicylate and the glycoprotein of Mr approximately 150 000 isolated on wheat germ agglutinin confirming their surface orientation. However, glycoprotein with Mr approximately 165 000 resolved by carboxymethylation of the lithium diiodosalicylate extracted glycoprotein mixture was not labelled by this method, suggesting that it represents the granule protein with similar electrophoretic characteristics described by others. Phosphorylation of intact platelets with 32Pi also results in labelling of glycoproteins isolated by both methods, suggesting that these molecules traverse the bilipid layer of the platelet membrane, bearing reactive groups on both outer and cytoplasmic surfaces."}
{"id": "PMID:486462", "title": "Cholinergic stimulants and excess potassium ion increase the fluidity of plasma membranes isolated from adrenal chromaffin cells.", "content": "Chromaffin cell membranes from the bovine adrenal medulla were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene, and the fluorescence polarization (P) of the membrane suspensions was measured as a function of temperature. The P versus t profiles, between 20 and 37 degrees C, showed two linear regions separated by a break in the vicinity of 30 degrees C, reflecting a change in the phase behaviour of the constitutent lipids. Decreases in P values at higher temperature indicated progressive fluidization of the lipid bilayer. Previous incubation with either acetylcholine (0.5 mM) or nicotine (50 microM) produced further fluidization, the extent of which depended on the presence of added Ca2+ (2.2 mM). Thus, the flow activation energy, delta E, between approx. 30 and 37 degrees C was 9.1 kcal/mol for acetylcholine and 8.8 kcal/mol for acetylcholine plus Ca2+, as compared to 7.9 kcal/mol in the absence of acetylcholine and Ca2+. In the presence of nicotine, delta E was 11.4 kcal/mol when Ca2+ was absent and 9.5 kcal/mol when it was present. The cholinergic blocker, hexamethonium (0.5 mM), abolished the acetylcholine- or nicotine-induced changes. 65 mM K+ produced a similar fluidization, which was reversed by addition of Ca2+. An additive effect was observed when the membranes were incubated with both nicotine and K+, with delta E = 16.6 kcal/mol in the presence of Cas2+. These results indicate a receptor-mediated modulation of the lipid distribution between rigid and fluid regions in the membrane, which could be of importance for stimulated catecholamine secretion in the intact cell.", "contents": "Cholinergic stimulants and excess potassium ion increase the fluidity of plasma membranes isolated from adrenal chromaffin cells. Chromaffin cell membranes from the bovine adrenal medulla were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene, and the fluorescence polarization (P) of the membrane suspensions was measured as a function of temperature. The P versus t profiles, between 20 and 37 degrees C, showed two linear regions separated by a break in the vicinity of 30 degrees C, reflecting a change in the phase behaviour of the constitutent lipids. Decreases in P values at higher temperature indicated progressive fluidization of the lipid bilayer. Previous incubation with either acetylcholine (0.5 mM) or nicotine (50 microM) produced further fluidization, the extent of which depended on the presence of added Ca2+ (2.2 mM). Thus, the flow activation energy, delta E, between approx. 30 and 37 degrees C was 9.1 kcal/mol for acetylcholine and 8.8 kcal/mol for acetylcholine plus Ca2+, as compared to 7.9 kcal/mol in the absence of acetylcholine and Ca2+. In the presence of nicotine, delta E was 11.4 kcal/mol when Ca2+ was absent and 9.5 kcal/mol when it was present. The cholinergic blocker, hexamethonium (0.5 mM), abolished the acetylcholine- or nicotine-induced changes. 65 mM K+ produced a similar fluidization, which was reversed by addition of Ca2+. An additive effect was observed when the membranes were incubated with both nicotine and K+, with delta E = 16.6 kcal/mol in the presence of Cas2+. These results indicate a receptor-mediated modulation of the lipid distribution between rigid and fluid regions in the membrane, which could be of importance for stimulated catecholamine secretion in the intact cell."}
{"id": "PMID:486463", "title": "Induction of gel-phase lipid in plasma membrane of chick intestinal cells after coccidial infection.", "content": "When chickens are infected with the coccidial parasite Eimeria necatrix, the plasma membrane of intestinal cells harbouring second-generation schizonts becomes refractory to mechanical shearing, hypotonic shock and ultrasonication. Plasma membrane from these infected cells was isolated to high purity as judged by enriched levels of ouabain-sensitive (Na+ + K+)-stimulated Mg2-dependent ATPase activity and sialic acid content, the lack of detectable cytochrome oxidase and glucose-6-phosphatase activities and electron microscopic analysis of the final preparation. Wide-angle X-ray diffraction patterns recorded from the isolated membranes revealed that during the later stages of parasite maturation the host cell plasma membrane acquires increasing proportions of gel-phase lipid. By contrast, purified membrane from isolated parasites is in a liquid-crystalline state. The transition temperature of host cell plasmalemma at 100 h postinfection is 61 degrees C, about 20 degrees C above physiological temperature. By contrast, liposomes of plasma membranes from infected cells undergo a thermal transition at about 28 degrees C. The accumulation of gel-phase lipid in the host cell plasma membrane is not attributable either to an increase in the constituent ratio of saturated to unsaturated fatty acids or to a significant change in the cholesterol to phospholipid ratio. During the late stages of infection, the cells become stainable with trypan blue which suggests that the acquisition of crystalline phase lipid disrupts the permeability of the host cell plasmalemma.", "contents": "Induction of gel-phase lipid in plasma membrane of chick intestinal cells after coccidial infection. When chickens are infected with the coccidial parasite Eimeria necatrix, the plasma membrane of intestinal cells harbouring second-generation schizonts becomes refractory to mechanical shearing, hypotonic shock and ultrasonication. Plasma membrane from these infected cells was isolated to high purity as judged by enriched levels of ouabain-sensitive (Na+ + K+)-stimulated Mg2-dependent ATPase activity and sialic acid content, the lack of detectable cytochrome oxidase and glucose-6-phosphatase activities and electron microscopic analysis of the final preparation. Wide-angle X-ray diffraction patterns recorded from the isolated membranes revealed that during the later stages of parasite maturation the host cell plasma membrane acquires increasing proportions of gel-phase lipid. By contrast, purified membrane from isolated parasites is in a liquid-crystalline state. The transition temperature of host cell plasmalemma at 100 h postinfection is 61 degrees C, about 20 degrees C above physiological temperature. By contrast, liposomes of plasma membranes from infected cells undergo a thermal transition at about 28 degrees C. The accumulation of gel-phase lipid in the host cell plasma membrane is not attributable either to an increase in the constituent ratio of saturated to unsaturated fatty acids or to a significant change in the cholesterol to phospholipid ratio. During the late stages of infection, the cells become stainable with trypan blue which suggests that the acquisition of crystalline phase lipid disrupts the permeability of the host cell plasmalemma."}
{"id": "PMID:486464", "title": "Distribution of insulin receptors among mouse liver endomembranes.", "content": "Specific binding of insulin to highly purified preparations of rough endoplasmic reticulum, Golgi apparatus, and plasma membrane of mouse liver was determined. 125I-labeled insulin bound maximally to the plasma membrane in radio-receptor assays. Golgi apparatus fractions exhibited binding 10--20% that of plasma membrane and rough endoplasmic reticulum exhibited only 1--2% of plasma membrane binding. Binding was proportional to membrane concentration and dose vs. response curves were very similar for the different fractions. Scatchard analysis of the insulin binding data for the plasma membrane and Golgi apparatus fractions showed curvilinear plots yielding similar apparent binding affinities (0.9 and 3.0-10(8) M-1, respectively). Purity of the isolated endomembranes was analyzed by morphometry and (Na+ + K+ + Mg2+)-ATPase and these preparations displayed less than 1% contamination by plasma membrane. These findings provide important confirmation of the presence of insulin receptors in Golgi apparatus membranes comparable to those located on the plasma membrane. Finally, the present study did not allow us to verify the existence of insulin receptors in the endoplasmic reticulum.", "contents": "Distribution of insulin receptors among mouse liver endomembranes. Specific binding of insulin to highly purified preparations of rough endoplasmic reticulum, Golgi apparatus, and plasma membrane of mouse liver was determined. 125I-labeled insulin bound maximally to the plasma membrane in radio-receptor assays. Golgi apparatus fractions exhibited binding 10--20% that of plasma membrane and rough endoplasmic reticulum exhibited only 1--2% of plasma membrane binding. Binding was proportional to membrane concentration and dose vs. response curves were very similar for the different fractions. Scatchard analysis of the insulin binding data for the plasma membrane and Golgi apparatus fractions showed curvilinear plots yielding similar apparent binding affinities (0.9 and 3.0-10(8) M-1, respectively). Purity of the isolated endomembranes was analyzed by morphometry and (Na+ + K+ + Mg2+)-ATPase and these preparations displayed less than 1% contamination by plasma membrane. These findings provide important confirmation of the presence of insulin receptors in Golgi apparatus membranes comparable to those located on the plasma membrane. Finally, the present study did not allow us to verify the existence of insulin receptors in the endoplasmic reticulum."}
{"id": "PMID:486465", "title": "Effects of bilirubin on potassium (86Rb+) influx and ionic content in Ehrlich ascites cells.", "content": "Potassium influx, intracellular potassium and sodium content and cellular volume were determined in vitro in Ehrlich ascites cells in the presence of up to 0.8 mM bilirubin in the incubation medium. Bilirubin uptake into cells as a function of bilirubin concentration in the incubation medium increased linearly with a molar bilirubin/albumin ratio of 20 : 1. Potassium influx and intracellular content decreased while cellular volume increased after 180 min of incubation of cells in bilirubin at a molar bilirubin/albumin ratio of 20 : 1. At a bilirubin/albumin ratio 2 : 1, potassium influx decreased, cellular volume remained unchanged, and bilirubin uptake into cells became saturated at bilirubin concentrations greater than 0.3 mM. It is suggested that bilirubin-induced alterations in potassium gradients across cell membranes may play a role in toxic effects of bilirubin on cells.", "contents": "Effects of bilirubin on potassium (86Rb+) influx and ionic content in Ehrlich ascites cells. Potassium influx, intracellular potassium and sodium content and cellular volume were determined in vitro in Ehrlich ascites cells in the presence of up to 0.8 mM bilirubin in the incubation medium. Bilirubin uptake into cells as a function of bilirubin concentration in the incubation medium increased linearly with a molar bilirubin/albumin ratio of 20 : 1. Potassium influx and intracellular content decreased while cellular volume increased after 180 min of incubation of cells in bilirubin at a molar bilirubin/albumin ratio of 20 : 1. At a bilirubin/albumin ratio 2 : 1, potassium influx decreased, cellular volume remained unchanged, and bilirubin uptake into cells became saturated at bilirubin concentrations greater than 0.3 mM. It is suggested that bilirubin-induced alterations in potassium gradients across cell membranes may play a role in toxic effects of bilirubin on cells."}
{"id": "PMID:486466", "title": "Current-voltage relationship of the basolateral membrane of a tight epithelium.", "content": "The polyene antibiotic nystatin is used to reduce selectively to zero the apical membrane resistance of the rabbit descending colon, allowing the measurement of the current-voltage curve of the basolateral membrane. The I--V relationship is described by the Goldman-Hodgkin-Katz equations allowing calculation of PNa/PK, PCl/PK and PK for the basolateral membrane. Cs+ is found to block inward current (serosa to mucosa) in a manner similar to that found in excitable membranes.", "contents": "Current-voltage relationship of the basolateral membrane of a tight epithelium. The polyene antibiotic nystatin is used to reduce selectively to zero the apical membrane resistance of the rabbit descending colon, allowing the measurement of the current-voltage curve of the basolateral membrane. The I--V relationship is described by the Goldman-Hodgkin-Katz equations allowing calculation of PNa/PK, PCl/PK and PK for the basolateral membrane. Cs+ is found to block inward current (serosa to mucosa) in a manner similar to that found in excitable membranes."}
{"id": "PMID:486467", "title": "The transport of L-cysteinesulfinate in rat liver mitochondria.", "content": "1. The mechanism of L-cysteinesulfinate permeation into rat liver mitochondria has been investigated. 2. Mitochondria do not swell in ammonium or potassium salts of L-cysteinesulfinate in all the conditions tested, including the presence of valinomycin and/or carbonylcyanide p-trifluoromethoxyphenylhydrazone. 3. The activation of malate oxidation by L-cysteinesulfinate is abolished by aminooxyacetate, an inhibitor of the intramitochondrial aspartate aminotransferase, it is not inhibited by high concentrations of carbonylcyanide p-trifluoromethoxyphenylhydrazone (in contrast to the oxidation of malate plus glutamate) and it is decreased on lowering the pH of the medium. 4. All the aspartate formed during the oxidation of malate plus L-cysteinesulfinate is exported into the extramitochondrial space. 5. Homocysteinesulfinate, cysteate and homocysteate, which are all good substrates of the mitochondrial aspartate aminotransferase, are unable to activate the oxidation of malate. Homocysteinesulfinate and homocysteate have no inhibitory effect on the L-cysteinesulfinate-induced respiration, whereas cysteate inhibits it competitively with respect to L-cysteinesulfinate. 6. In contrast to D-aspartate, D-cysteinesulfinate and D-glutamate, L-aspartate inhibits the oxidation of malate plus L-cysteinesulfinate in a competitive way with respect to L-cysteinesulfinate. Vice versa, L-cysteinesulfinate inhibits the influx of L-aspartate. 7. Externally added L-cysteinesulfinate elicits efflux of intramitochondrial L-aspartate or L-glutamate. The cysteinesulfinate analogues homocysteinesulfinate, cysteate and homocysteate and the D-stereoisomers of cysteinesulfinate, aspartate and glutamate do not cause a significant release of internal glutamate or aspartate, indicating a high degree of specificity of the exchange reactions. External L-cysteinesulfinate does not cause efflux of intramitochondrial Pi, malate, malonate, citrate, oxoglutarate, pyruvate or ADP. The L-cysteinesulfinate-aspartate and L-cysteinesulfinate-glutamate exchanges are inhibited by glisoxepide and by known substrates of the glutamate-aspartate carrier. 8. The exchange between external L-cysteinesulfinate and intramitochondrial glutamate is accompanied by translocation of protons across the mitochondrial membrane in the same direction as glutamate. The L-cysteinesulfinate-aspartate exchange, on the other hand, is not accompanied by H+ translocation. 9. The ratios delta H+/delta glutamate, delta L-cysteinesulfinate/delta glutamate and delta L-cysteinesulfinate/delta aspartate are close to unity. 10. It is concluded that L-cysteinesulfinate is transported by the glutamate-aspartate carrier of rat liver mitochondria. The present data suggest that the dissociated form of L-cysteinesulfinate exchanges with H+-compensated glutamate or with negatively charged aspartate.", "contents": "The transport of L-cysteinesulfinate in rat liver mitochondria. 1. The mechanism of L-cysteinesulfinate permeation into rat liver mitochondria has been investigated. 2. Mitochondria do not swell in ammonium or potassium salts of L-cysteinesulfinate in all the conditions tested, including the presence of valinomycin and/or carbonylcyanide p-trifluoromethoxyphenylhydrazone. 3. The activation of malate oxidation by L-cysteinesulfinate is abolished by aminooxyacetate, an inhibitor of the intramitochondrial aspartate aminotransferase, it is not inhibited by high concentrations of carbonylcyanide p-trifluoromethoxyphenylhydrazone (in contrast to the oxidation of malate plus glutamate) and it is decreased on lowering the pH of the medium. 4. All the aspartate formed during the oxidation of malate plus L-cysteinesulfinate is exported into the extramitochondrial space. 5. Homocysteinesulfinate, cysteate and homocysteate, which are all good substrates of the mitochondrial aspartate aminotransferase, are unable to activate the oxidation of malate. Homocysteinesulfinate and homocysteate have no inhibitory effect on the L-cysteinesulfinate-induced respiration, whereas cysteate inhibits it competitively with respect to L-cysteinesulfinate. 6. In contrast to D-aspartate, D-cysteinesulfinate and D-glutamate, L-aspartate inhibits the oxidation of malate plus L-cysteinesulfinate in a competitive way with respect to L-cysteinesulfinate. Vice versa, L-cysteinesulfinate inhibits the influx of L-aspartate. 7. Externally added L-cysteinesulfinate elicits efflux of intramitochondrial L-aspartate or L-glutamate. The cysteinesulfinate analogues homocysteinesulfinate, cysteate and homocysteate and the D-stereoisomers of cysteinesulfinate, aspartate and glutamate do not cause a significant release of internal glutamate or aspartate, indicating a high degree of specificity of the exchange reactions. External L-cysteinesulfinate does not cause efflux of intramitochondrial Pi, malate, malonate, citrate, oxoglutarate, pyruvate or ADP. The L-cysteinesulfinate-aspartate and L-cysteinesulfinate-glutamate exchanges are inhibited by glisoxepide and by known substrates of the glutamate-aspartate carrier. 8. The exchange between external L-cysteinesulfinate and intramitochondrial glutamate is accompanied by translocation of protons across the mitochondrial membrane in the same direction as glutamate. The L-cysteinesulfinate-aspartate exchange, on the other hand, is not accompanied by H+ translocation. 9. The ratios delta H+/delta glutamate, delta L-cysteinesulfinate/delta glutamate and delta L-cysteinesulfinate/delta aspartate are close to unity. 10. It is concluded that L-cysteinesulfinate is transported by the glutamate-aspartate carrier of rat liver mitochondria. The present data suggest that the dissociated form of L-cysteinesulfinate exchanges with H+-compensated glutamate or with negatively charged aspartate."}
{"id": "PMID:486468", "title": "Lipid distribution in Acholeplasma laidlawii membrane. A study using the lactoperoxidase-mediated iodination.", "content": "The lactoperoxidase-mediated radioiodination has been applied to study the transbilayer distribution of phospho- and glycolipids in Acholeplasma laidlawii membranes. After radioiodination, about 5% of the 125I-iodine was found in membrane lipids. A comparison of the labeling intensities of the various lipid species between iodinated intact cells and isolated membranes revealed that the glycolipids monoglucosyldiglyceride and diglucosyldiglyceride are located almost exclusively in the outer half of the bilayer, whereas the phospholipids phosphatidylglycerol and diphosphatidylglycerol as well as the phosphoglycolipids glycerophosphoryl-diglucosyldiglyceride and glycerophosphoryl-monoglucosyldiglyceride are almost equally distributed in the outer and inner halves of A. laidlawii membranes.", "contents": "Lipid distribution in Acholeplasma laidlawii membrane. A study using the lactoperoxidase-mediated iodination. The lactoperoxidase-mediated radioiodination has been applied to study the transbilayer distribution of phospho- and glycolipids in Acholeplasma laidlawii membranes. After radioiodination, about 5% of the 125I-iodine was found in membrane lipids. A comparison of the labeling intensities of the various lipid species between iodinated intact cells and isolated membranes revealed that the glycolipids monoglucosyldiglyceride and diglucosyldiglyceride are located almost exclusively in the outer half of the bilayer, whereas the phospholipids phosphatidylglycerol and diphosphatidylglycerol as well as the phosphoglycolipids glycerophosphoryl-diglucosyldiglyceride and glycerophosphoryl-monoglucosyldiglyceride are almost equally distributed in the outer and inner halves of A. laidlawii membranes."}
{"id": "PMID:486469", "title": "The affinity of cholesterol for phosphatidylcholine and sphingomyelin.", "content": "Erythrocyte ghosts were incubated with sonicated vesicles and the uptake of cholesterol by vesicles allowed to proceed to equilibrium. The experiments were carried out for a series of phospholipids at different temperatures. The equilibrium partition of cholesterol between ghosts and single shelled vesicles provided a measure of the relative affinities of cholesterol for the different phospholipids studied. It was found that the affinity of cholesterol for dipalmitoyl phosphatidylcholine was the same as that for N-palmitoyl sphingomyelin both at temperatures above and below the gel to liquid crystalline transition temperature of these phospholipids.", "contents": "The affinity of cholesterol for phosphatidylcholine and sphingomyelin. Erythrocyte ghosts were incubated with sonicated vesicles and the uptake of cholesterol by vesicles allowed to proceed to equilibrium. The experiments were carried out for a series of phospholipids at different temperatures. The equilibrium partition of cholesterol between ghosts and single shelled vesicles provided a measure of the relative affinities of cholesterol for the different phospholipids studied. It was found that the affinity of cholesterol for dipalmitoyl phosphatidylcholine was the same as that for N-palmitoyl sphingomyelin both at temperatures above and below the gel to liquid crystalline transition temperature of these phospholipids."}
{"id": "PMID:486470", "title": "Membrane lipid fluidity as rate limiting in the concanavalin A-mediated agglutination of pyBHK cells.", "content": "The initial rate of concanavalin A-mediated agglutination of polyoma transformed Baby Hamster Kidney (pyBHK) cells follows Arrhenius kinetics. There is a smooth decrease in the agglutination rate from 37 degrees C to 22 degrees C with an activation energy of 11.8 +/- 0.2 kcal/mol in this region. There is a sharp decrease in agglutination rate below 22 degrees C. The addition of 0.1 mM 1,3-di-tert-2-hydroxyl-5-methylbenzene, a lipid perturber, increases the agglutination rate by a factor of two and increases the membrane lipid fluidity as determined by the spin label method. The rotational correlation time of the spin label 2N14 (2,2-dimethyl-5-dodecyl-5-methyloxazolidine-N-oxide) was measured. The sum of the enthalpy of activation of rotational diffusion and the enthalpy of activation of translational diffusion is very nearly equal to the enthalpy of activation of agglutination. This is consistent with the rate limiting step of agglutination being receptor diffusion, which is probably limited in pyBHK cells by membrane lipid fluidity.", "contents": "Membrane lipid fluidity as rate limiting in the concanavalin A-mediated agglutination of pyBHK cells. The initial rate of concanavalin A-mediated agglutination of polyoma transformed Baby Hamster Kidney (pyBHK) cells follows Arrhenius kinetics. There is a smooth decrease in the agglutination rate from 37 degrees C to 22 degrees C with an activation energy of 11.8 +/- 0.2 kcal/mol in this region. There is a sharp decrease in agglutination rate below 22 degrees C. The addition of 0.1 mM 1,3-di-tert-2-hydroxyl-5-methylbenzene, a lipid perturber, increases the agglutination rate by a factor of two and increases the membrane lipid fluidity as determined by the spin label method. The rotational correlation time of the spin label 2N14 (2,2-dimethyl-5-dodecyl-5-methyloxazolidine-N-oxide) was measured. The sum of the enthalpy of activation of rotational diffusion and the enthalpy of activation of translational diffusion is very nearly equal to the enthalpy of activation of agglutination. This is consistent with the rate limiting step of agglutination being receptor diffusion, which is probably limited in pyBHK cells by membrane lipid fluidity."}
{"id": "PMID:486471", "title": "Effect of different physical states of phospholipid substrates on partially purified platelet phospholipase A2 activity.", "content": "Partial purification of alkaline phospholipase A2 (EC 3.1.1.4) from rabbit platelets was carried out and the effect of different physical states of the substrate phosphatidylcholine on the activity was investigated. (1) The enzyme was purified about 1020-fold by means of Sephadex gel chromatography after extraction from a particulate fraction of rabbit platelets, followed by CM-cellulose chromatography, and had a molecular weight of approx. 12 000 as determined by gel chromatography. (2) The activity of the purified enzyme was enhanced by the addition of detergents. Sodium deoxycholate and sodium cholate markedly stimulated the activity, and the effect of these substances was observed well below the critical micelle concentrations. Triton X-100 stimulated the activity moderately, and the activation was observed only above the critical micelle concentration. (3) The addition of negatively charged phospholipids to the substrate egg phosphatidylcholine induced a moderate activation of hydrolysis. (4) The addition of long-chain cation to the substrate induced an inhibition of the activity, whereas the addition of long-chain anion activated the hydrolysis of egg phosphatidylcholine, but did not activate the hydrolysis of phosphatidylcholine in the total lipid extract of rabbit platelets. (5) Hydrolysis of dimyristoyl phosphatidylcholine increased in the temperature region of the phase transition of the substrate. Addition of cholesterol at the concentration of 20 mol% diminished the effect of phase transition. (6) Release of [1-14C]arachidonic acid from an equimolar mixture of egg phosphatidylcholine with dipalmitoyl or distearoyl phosphatidylcholine was activated at the temperature of 0 degrees C or 20 degrees C, respectively. From these results, we suggest that platelet phospholipase A2 can be activated to release fatty acids from the platelet phospholipids at the domains within membranes, where exist the structural irregularities and/or accumulation of negative charge within the bilayers.", "contents": "Effect of different physical states of phospholipid substrates on partially purified platelet phospholipase A2 activity. Partial purification of alkaline phospholipase A2 (EC 3.1.1.4) from rabbit platelets was carried out and the effect of different physical states of the substrate phosphatidylcholine on the activity was investigated. (1) The enzyme was purified about 1020-fold by means of Sephadex gel chromatography after extraction from a particulate fraction of rabbit platelets, followed by CM-cellulose chromatography, and had a molecular weight of approx. 12 000 as determined by gel chromatography. (2) The activity of the purified enzyme was enhanced by the addition of detergents. Sodium deoxycholate and sodium cholate markedly stimulated the activity, and the effect of these substances was observed well below the critical micelle concentrations. Triton X-100 stimulated the activity moderately, and the activation was observed only above the critical micelle concentration. (3) The addition of negatively charged phospholipids to the substrate egg phosphatidylcholine induced a moderate activation of hydrolysis. (4) The addition of long-chain cation to the substrate induced an inhibition of the activity, whereas the addition of long-chain anion activated the hydrolysis of egg phosphatidylcholine, but did not activate the hydrolysis of phosphatidylcholine in the total lipid extract of rabbit platelets. (5) Hydrolysis of dimyristoyl phosphatidylcholine increased in the temperature region of the phase transition of the substrate. Addition of cholesterol at the concentration of 20 mol% diminished the effect of phase transition. (6) Release of [1-14C]arachidonic acid from an equimolar mixture of egg phosphatidylcholine with dipalmitoyl or distearoyl phosphatidylcholine was activated at the temperature of 0 degrees C or 20 degrees C, respectively. From these results, we suggest that platelet phospholipase A2 can be activated to release fatty acids from the platelet phospholipids at the domains within membranes, where exist the structural irregularities and/or accumulation of negative charge within the bilayers."}
{"id": "PMID:486472", "title": "Asymmetric distribution of arachidonic acid in the plasma membrane of human platelets. A determination using purified phospholipases and a rapid method for membrane isolation.", "content": "1. Non-lytic degradation of human platelet phospholipids have been performed using a combination of bee venom phospholipase A2 (phosphatide 2-acyl-hydrolase, EC 3.1.1.4) and Staphylococcus aureus sphingomyelinase C (sphingomyelin choline phosphohydrolase). Under these conditions, 25.4% of total phospholipds are degraded and 6.4% of total platelet arachidonic acid is released. 2. A new method for rapid isolation of platelet plasma membrane is described, based on the use of [3H]concanavalin A as a membrane marker and of self-generating gradients of Percoll. Plasma membranes are enriched 5.2 fold in lectin marker and 0.43 in N-acetyl-beta-D-glucosaminidase, the main contaminant. This method allows to estimate that 57% of the total cell phospholipids and 61% of the total arachidonic acid content are located in the plasma membrane. 3. The distribution of phospholipids and arachidonic acid between the two leaflets of the plasma membrane has been deduced by using these values and those obtained from non-lytic treatment of intact platelets by phospholipases. It is concluded that 45% of plasma membrane phospholipids, comprising 93% of sphingomyelin, 45% of phosphatidylcholine, 9% of phosphatidylserine, 16% of phosphatidylinositol and 20% of phosphatidylethanolamine form the outer half of the human platelet plasma membrane. The phospholipids appear to bear only 10% of the total membrane arachidonic acid.", "contents": "Asymmetric distribution of arachidonic acid in the plasma membrane of human platelets. A determination using purified phospholipases and a rapid method for membrane isolation. 1. Non-lytic degradation of human platelet phospholipids have been performed using a combination of bee venom phospholipase A2 (phosphatide 2-acyl-hydrolase, EC 3.1.1.4) and Staphylococcus aureus sphingomyelinase C (sphingomyelin choline phosphohydrolase). Under these conditions, 25.4% of total phospholipds are degraded and 6.4% of total platelet arachidonic acid is released. 2. A new method for rapid isolation of platelet plasma membrane is described, based on the use of [3H]concanavalin A as a membrane marker and of self-generating gradients of Percoll. Plasma membranes are enriched 5.2 fold in lectin marker and 0.43 in N-acetyl-beta-D-glucosaminidase, the main contaminant. This method allows to estimate that 57% of the total cell phospholipids and 61% of the total arachidonic acid content are located in the plasma membrane. 3. The distribution of phospholipids and arachidonic acid between the two leaflets of the plasma membrane has been deduced by using these values and those obtained from non-lytic treatment of intact platelets by phospholipases. It is concluded that 45% of plasma membrane phospholipids, comprising 93% of sphingomyelin, 45% of phosphatidylcholine, 9% of phosphatidylserine, 16% of phosphatidylinositol and 20% of phosphatidylethanolamine form the outer half of the human platelet plasma membrane. The phospholipids appear to bear only 10% of the total membrane arachidonic acid."}
{"id": "PMID:486473", "title": "Developmental changes in proteins of purified membranes of chicken lenses and evidence for contamination by cytoplasmic delta-crystallin.", "content": "Urea-washed membranes from embryonic chick lenses (15 days old) and from the cortical and nuclear regions of adult chicken lenses (1 year) have been prepared by repeated centrifugation through discontinuous density gradients. The protein components of the isolated membranes have been examined by electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate and urea. Proteins with molecular weights of 75 000, 56 000, 54 000, 48 000, 34 000, 32 000, 25 000, and 22 000 were present in all the membrane preparations, although their proportions changed during development. One additional protein, molecular weight 70 000, was seen only in the embryonic lens membranes. The greatest developmental change was the increase in 25 000 molecular weight protein from 12% in the embryonic lens to about 45% in the adult lens. Since it has been suggested that this protein is associated with gap junctions, its increase during development may reflect a corresponding increase in the number of gap junctions in the lens. The 50 000 molecular weight protein of embryonic lens membranes and membranes of adult nuclear lens fibers consisted at least partly of delta-crystallin, since delta-crystallin peptides could be identified in tryptic peptide maps of the isolated protein after in vitro radioiodination. Peptide maps of the 50 000 molecular weight protein of cortical lens fiber membranes contained no identifiable delta-crystallin peptides, although it is possible that modified delta-crystallin peptides may be present. The level of cytoplasmic contamination of the membrane fraction was estimated by preparing lens membranes in the presence of added delta-[35S]crystallin. The results indicated that cytoplasmic contamination contributes significantly to the presence of delta-crystallin in lens membrane preparations.", "contents": "Developmental changes in proteins of purified membranes of chicken lenses and evidence for contamination by cytoplasmic delta-crystallin. Urea-washed membranes from embryonic chick lenses (15 days old) and from the cortical and nuclear regions of adult chicken lenses (1 year) have been prepared by repeated centrifugation through discontinuous density gradients. The protein components of the isolated membranes have been examined by electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate and urea. Proteins with molecular weights of 75 000, 56 000, 54 000, 48 000, 34 000, 32 000, 25 000, and 22 000 were present in all the membrane preparations, although their proportions changed during development. One additional protein, molecular weight 70 000, was seen only in the embryonic lens membranes. The greatest developmental change was the increase in 25 000 molecular weight protein from 12% in the embryonic lens to about 45% in the adult lens. Since it has been suggested that this protein is associated with gap junctions, its increase during development may reflect a corresponding increase in the number of gap junctions in the lens. The 50 000 molecular weight protein of embryonic lens membranes and membranes of adult nuclear lens fibers consisted at least partly of delta-crystallin, since delta-crystallin peptides could be identified in tryptic peptide maps of the isolated protein after in vitro radioiodination. Peptide maps of the 50 000 molecular weight protein of cortical lens fiber membranes contained no identifiable delta-crystallin peptides, although it is possible that modified delta-crystallin peptides may be present. The level of cytoplasmic contamination of the membrane fraction was estimated by preparing lens membranes in the presence of added delta-[35S]crystallin. The results indicated that cytoplasmic contamination contributes significantly to the presence of delta-crystallin in lens membrane preparations."}
{"id": "PMID:486474", "title": "Constraint on the substrate cytochrome P-450 binding reaction in bovine adrenocortical microsomes at physiological temperature.", "content": "The addition of cholate to the microsomes at 37.5 degrees C resulted in a striking decrease in the apparent substrate dissociation constant (K's) and its temperature dependency. The microsomal membranes depleted of 80% of the lipids preserved the temperature dependency of the Ks and exhibited breaks in the Van't Hoff plot at the characteristic temperature of the lipids phase transition. The results indicate that the cytochrome P-450 is considerably restrained from expressing its maximum substrate binding potential at physiological temperature. In addition, the results indicate that the majority of the lipids apparently do not play a significant role in imposing constraint on the substrate-cytochrome -450 binding reaction and in the temperature dependency of the Ks.", "contents": "Constraint on the substrate cytochrome P-450 binding reaction in bovine adrenocortical microsomes at physiological temperature. The addition of cholate to the microsomes at 37.5 degrees C resulted in a striking decrease in the apparent substrate dissociation constant (K's) and its temperature dependency. The microsomal membranes depleted of 80% of the lipids preserved the temperature dependency of the Ks and exhibited breaks in the Van't Hoff plot at the characteristic temperature of the lipids phase transition. The results indicate that the cytochrome P-450 is considerably restrained from expressing its maximum substrate binding potential at physiological temperature. In addition, the results indicate that the majority of the lipids apparently do not play a significant role in imposing constraint on the substrate-cytochrome -450 binding reaction and in the temperature dependency of the Ks."}
{"id": "PMID:486475", "title": "A simple experimental approach to the determination of carrier transport parameters for unlabeled substrate analogs.", "content": "A method is described by which affinities and transport rates for unlabeled substrate analogs are readily determined, and which is based on the effect of an unlabeled analog upon the rate of transport of a labeled substrate present at a low concentration on the trans side of the membrane. The procedure is widely applicable since it does not depend on assumptions about rrate-limiting steps and holds for both active and non-active systems. Here it is applied in an experimental study of the facilitated diffusion system for choline in erythrocytes, and it is shown that the transport parameters for a test substrate obtained by this method are the same as those found when the transport of the substrate is followed directly.", "contents": "A simple experimental approach to the determination of carrier transport parameters for unlabeled substrate analogs. A method is described by which affinities and transport rates for unlabeled substrate analogs are readily determined, and which is based on the effect of an unlabeled analog upon the rate of transport of a labeled substrate present at a low concentration on the trans side of the membrane. The procedure is widely applicable since it does not depend on assumptions about rrate-limiting steps and holds for both active and non-active systems. Here it is applied in an experimental study of the facilitated diffusion system for choline in erythrocytes, and it is shown that the transport parameters for a test substrate obtained by this method are the same as those found when the transport of the substrate is followed directly."}
{"id": "PMID:486476", "title": "A general kinetic analysis of transport. Tests of the carrier model based on predicted relations among experimental parameters.", "content": "The analysis of transport kinetics has lacked both a unified treatment in which general rate equations are written entirely in terms of experimental parameters, and a convention by which these parameters may be designated in a concise yet immediately recognizable manner. Such a treatment is presented here in an easily accessible form, and a simple system of nomenclature is proposed resembling that in use in enzyme kinetics. The treatment is independent of assumptions about rate-limiting steps in transport, and applies to both active and facilitated systems, including obligatory exchange. A single substrate is characterized by twelve different parameters, only five of which are required in theory to calculate the others. If a second substrate is present on the trans side of the membrane there are six more parameters. All eighteen parameters are linked by multiple relationships which provide a complete set of rejection criteria for the generalized form of the mobile carrier. Relationships among parameters are also defined that give information on the rate-limiting steps in transport. Equations governing any individual experiment, involving only experimental parameters, are easily written out from the general expressions, for example under conditions of zero trans and infinite trans flux, equilibrium exchange, or competitive inhibition.", "contents": "A general kinetic analysis of transport. Tests of the carrier model based on predicted relations among experimental parameters. The analysis of transport kinetics has lacked both a unified treatment in which general rate equations are written entirely in terms of experimental parameters, and a convention by which these parameters may be designated in a concise yet immediately recognizable manner. Such a treatment is presented here in an easily accessible form, and a simple system of nomenclature is proposed resembling that in use in enzyme kinetics. The treatment is independent of assumptions about rate-limiting steps in transport, and applies to both active and facilitated systems, including obligatory exchange. A single substrate is characterized by twelve different parameters, only five of which are required in theory to calculate the others. If a second substrate is present on the trans side of the membrane there are six more parameters. All eighteen parameters are linked by multiple relationships which provide a complete set of rejection criteria for the generalized form of the mobile carrier. Relationships among parameters are also defined that give information on the rate-limiting steps in transport. Equations governing any individual experiment, involving only experimental parameters, are easily written out from the general expressions, for example under conditions of zero trans and infinite trans flux, equilibrium exchange, or competitive inhibition."}
{"id": "PMID:486477", "title": "Models of interaction between nucleic acids and proteins. Hydrogen bonding of arginine with nucleic acid bases, phosphate groups and carboxylic acids.", "content": "Complex formation between the side chain of arginine and nucleic acid bases has been investigated by proton magnetic resonance in dimethylsulfoxide. Simultaneous formation of two hydrogen bonds leads to a selectivity of arginine interaction towards cytosine and guanine. A comparison is made of the interaction of arginine side chain with nucleic acid bases, phosphate and carboxylate anions. It is shown that interaction between carboxylate and arginine is stronger than between phosphate and arginine. These results are discussed with respect to the selective recognition of nucleic acid bases by arginine side chains and by the arginyl-glutamyl ion pair which could form in proteins interacting with nucleic acids.", "contents": "Models of interaction between nucleic acids and proteins. Hydrogen bonding of arginine with nucleic acid bases, phosphate groups and carboxylic acids. Complex formation between the side chain of arginine and nucleic acid bases has been investigated by proton magnetic resonance in dimethylsulfoxide. Simultaneous formation of two hydrogen bonds leads to a selectivity of arginine interaction towards cytosine and guanine. A comparison is made of the interaction of arginine side chain with nucleic acid bases, phosphate and carboxylate anions. It is shown that interaction between carboxylate and arginine is stronger than between phosphate and arginine. These results are discussed with respect to the selective recognition of nucleic acid bases by arginine side chains and by the arginyl-glutamyl ion pair which could form in proteins interacting with nucleic acids."}
{"id": "PMID:486478", "title": "The activity and sedimentation properties of the aminoacyl-tRNA synthetases of rat skeletal muscle.", "content": "The aminoacyl-tRNA synthetases of the postribosomal supernatant fraction of rat skeletal muscle were characterized by their activity and sedimentation properties. The synthetases of muscle were compared with those of liver in terms of these parameters. Extraction of the synthetases of muscle with a buffer containing 4 mM adenosine triphosphate (ATP) resulted in a 50--100% increase in the activities of glutaminyl-, glutamyl-, isoleucyl-, leucyl-, lysyl-, methionyl-, and prolyl-tRNA synthetases in the postribosomal fraction, over those activities extracted in the absence of ATP. This effect of ATP was specific for those synthetases which sedimented as particulate elements in sucrose gradients, and appeared to be unique to muscle. The individual synthetase activities of muscle, except alanyl-, leucyl-, and valyl-tRNA synthetases, were aprrox. 25% of the corresponding synthetase activities of liver. Sucrose density gradient analysis of the postribosomal fraction of muscle and liver revealed that the sedimentation profiles of the synthetases of the two tissues were similar, with nine synthetase activities sedimenting as large particulate entities at 18 S. The findings suggest that the particulate forms of the synthetases reflect true association of the enzymes with a high molecular weight cellular component common to both tissues.", "contents": "The activity and sedimentation properties of the aminoacyl-tRNA synthetases of rat skeletal muscle. The aminoacyl-tRNA synthetases of the postribosomal supernatant fraction of rat skeletal muscle were characterized by their activity and sedimentation properties. The synthetases of muscle were compared with those of liver in terms of these parameters. Extraction of the synthetases of muscle with a buffer containing 4 mM adenosine triphosphate (ATP) resulted in a 50--100% increase in the activities of glutaminyl-, glutamyl-, isoleucyl-, leucyl-, lysyl-, methionyl-, and prolyl-tRNA synthetases in the postribosomal fraction, over those activities extracted in the absence of ATP. This effect of ATP was specific for those synthetases which sedimented as particulate elements in sucrose gradients, and appeared to be unique to muscle. The individual synthetase activities of muscle, except alanyl-, leucyl-, and valyl-tRNA synthetases, were aprrox. 25% of the corresponding synthetase activities of liver. Sucrose density gradient analysis of the postribosomal fraction of muscle and liver revealed that the sedimentation profiles of the synthetases of the two tissues were similar, with nine synthetase activities sedimenting as large particulate entities at 18 S. The findings suggest that the particulate forms of the synthetases reflect true association of the enzymes with a high molecular weight cellular component common to both tissues."}
{"id": "PMID:486479", "title": "Evolutionary aspects of variant types of rat mitochondrial DNA'S.", "content": "Mitochondrial DNA's (mtDNAs) were prepared from various kinds of individual Norway rats, Rattus norvegicus, and from three types of individual black rats, Rattus rattus, (Asian type, Ceylon type, and Oceanian type). Intra- and interspecies divergence of their mtDNA sequences were calculated based on changes in restriction endonuclease cleavage sites. The extent of intraspecies divergence of black rats (about 8%) is much larger than that of Norway rats (1%) and the mtDNA of Asian-type black rats resembles the mtDNA of Norway rats more closely than it resembles the mtDNA of other types of black rats. These results strongly suggest that during the course of intraspecies differentiation of black rats, probably long after the separation of the three types of black rats, some Asian-type black rats were isolated sexually and formed a new species, Norway rats. On the basis of our observations we propose a hypothetical process to explain the evolution of animal mtDNA.", "contents": "Evolutionary aspects of variant types of rat mitochondrial DNA'S. Mitochondrial DNA's (mtDNAs) were prepared from various kinds of individual Norway rats, Rattus norvegicus, and from three types of individual black rats, Rattus rattus, (Asian type, Ceylon type, and Oceanian type). Intra- and interspecies divergence of their mtDNA sequences were calculated based on changes in restriction endonuclease cleavage sites. The extent of intraspecies divergence of black rats (about 8%) is much larger than that of Norway rats (1%) and the mtDNA of Asian-type black rats resembles the mtDNA of Norway rats more closely than it resembles the mtDNA of other types of black rats. These results strongly suggest that during the course of intraspecies differentiation of black rats, probably long after the separation of the three types of black rats, some Asian-type black rats were isolated sexually and formed a new species, Norway rats. On the basis of our observations we propose a hypothetical process to explain the evolution of animal mtDNA."}
{"id": "PMID:486480", "title": "Control of transcription and translation by low molecular weight peptides (deprimerones) from chromatin and poly(A)-messenger RNA. Implication in the mechanism of carcinogenesis.", "content": "Poly(A)-mRNA isolated by phenol/chloroform extraction of rat liver polysomes, subtilism digestion, and poly(U)-Sepharose chromatography, contains a low molecular weight (approx. 1000) peptidic fraction. The peptides were extracted from a poly(A)-mRNA fraction by treatment with 80% ethanol; after ethanol evaporation they were purified on a Sephadex G-25 column and high-performance liquid chromatography on muBondapak C18. The isolated peptides were analyzed by cellulose gel thin-layer chromatography, high-pressure liquid chromatography and their amino acid composition was determined. They were compared with a chromatin peptidic fraction isolated from calf thymus nucleic by affinity chromatography on DNA-cellulose or on Sephadex G-25 column. Both groups of peptides from chromatin and from poly(A)-mRNA bind to the purified DNA thereby increasing its melting point; they significantly inhibit DNA transcription and RNA translation in reconstituted cell-free, peptide-free systems. It is suggested that these peptides are endogenous natural regulatory substances controlling gene expression in eucaryotic cells. We propose to name these regulatory peptides 'deprimerones' (from Latin 'deprimere') and describe various fractions of them as chromatin deprimerones, messenger deprimerones, gene deprimerones (for specific genes). Loss or decreased level of these deprimerones during the promotion of carcinogenesis is responsible for uncontrolled gene expression observed in cancer.", "contents": "Control of transcription and translation by low molecular weight peptides (deprimerones) from chromatin and poly(A)-messenger RNA. Implication in the mechanism of carcinogenesis. Poly(A)-mRNA isolated by phenol/chloroform extraction of rat liver polysomes, subtilism digestion, and poly(U)-Sepharose chromatography, contains a low molecular weight (approx. 1000) peptidic fraction. The peptides were extracted from a poly(A)-mRNA fraction by treatment with 80% ethanol; after ethanol evaporation they were purified on a Sephadex G-25 column and high-performance liquid chromatography on muBondapak C18. The isolated peptides were analyzed by cellulose gel thin-layer chromatography, high-pressure liquid chromatography and their amino acid composition was determined. They were compared with a chromatin peptidic fraction isolated from calf thymus nucleic by affinity chromatography on DNA-cellulose or on Sephadex G-25 column. Both groups of peptides from chromatin and from poly(A)-mRNA bind to the purified DNA thereby increasing its melting point; they significantly inhibit DNA transcription and RNA translation in reconstituted cell-free, peptide-free systems. It is suggested that these peptides are endogenous natural regulatory substances controlling gene expression in eucaryotic cells. We propose to name these regulatory peptides 'deprimerones' (from Latin 'deprimere') and describe various fractions of them as chromatin deprimerones, messenger deprimerones, gene deprimerones (for specific genes). Loss or decreased level of these deprimerones during the promotion of carcinogenesis is responsible for uncontrolled gene expression observed in cancer."}
{"id": "PMID:486481", "title": "Cucumber mosaic virus-associated RNA 5. VI. Characterization and denaturation-renaturation behavior of the double-stranded form.", "content": "The double-stranded form of cucumber mosaic virus-associated RNA 5 has been purified and further characterized. Its molecular weight determined by sedimentation equilibrium is 2.15 . 10(5). The buoyant density calculated from its symmetrical distribution in Cs2SO4, following isopycnic ultracentrifugation, is 1.615 g/cm3. The sedimentation rate of double-stranded cucumber mosaic virus-associated RNA 5 is slightly greater than that of cucumber mosaic virus-associated RNA 5; its electrophoretic mobility in polyacrylamide gel (2.4%) is less than that of cucumber mosaic virus-associated RNA 5. By the above standards the double-stranded cucumber mosaic virus-associated RNA 5 preparations used were found to be nomogeneous in size as well as density. Thermal denaturation monitored by means of ultraviolet light absorption produced multitransitional denaturation profiles. The average melting temperature (Tm) was 88 degrees C in 0.1 x SSC. Monotransitional denaturation profiles and slightly higher Tm values were obtained when resistance against ribonuclease digestion was measured. These denaturation experiments and other propertied led to the conclusion that double-stranded cucumber mosaic virus-associated RNA 5 and the double-stranded form of peanut stunt virus-associated RNA 5 are small double-stranded nucleic acids with several homostable base-pair regions, characterized by distinct G + C contents and Tm values.", "contents": "Cucumber mosaic virus-associated RNA 5. VI. Characterization and denaturation-renaturation behavior of the double-stranded form. The double-stranded form of cucumber mosaic virus-associated RNA 5 has been purified and further characterized. Its molecular weight determined by sedimentation equilibrium is 2.15 . 10(5). The buoyant density calculated from its symmetrical distribution in Cs2SO4, following isopycnic ultracentrifugation, is 1.615 g/cm3. The sedimentation rate of double-stranded cucumber mosaic virus-associated RNA 5 is slightly greater than that of cucumber mosaic virus-associated RNA 5; its electrophoretic mobility in polyacrylamide gel (2.4%) is less than that of cucumber mosaic virus-associated RNA 5. By the above standards the double-stranded cucumber mosaic virus-associated RNA 5 preparations used were found to be nomogeneous in size as well as density. Thermal denaturation monitored by means of ultraviolet light absorption produced multitransitional denaturation profiles. The average melting temperature (Tm) was 88 degrees C in 0.1 x SSC. Monotransitional denaturation profiles and slightly higher Tm values were obtained when resistance against ribonuclease digestion was measured. These denaturation experiments and other propertied led to the conclusion that double-stranded cucumber mosaic virus-associated RNA 5 and the double-stranded form of peanut stunt virus-associated RNA 5 are small double-stranded nucleic acids with several homostable base-pair regions, characterized by distinct G + C contents and Tm values."}
{"id": "PMID:486482", "title": "Conformational analysis of arabinonucleosides and nucleotides. A comparison with the ribonucleosides and nucleotides.", "content": "Conformations of arabino nucleosides and nucleotides have been analyzed by semiempirical energy calculations. It is found that the change in the configuration of the O(2')-hydroxyl group in arabinoses compared to riboses exerts significant influence on the conformational priorities of the glycosyl and the exocyclic C(4')-C(5') bond torsions. While the anti conformations for the bases are preferred, the anti in equilibrium or formed from syn interconversion is considerably hampered compared to ribosides due to large energy barrier. Further the preferred anti glycosyl torsions are shifted to higher values for C(3')-endo puckers and in ribosides. While the gauche+ conformation around the C(4')-C(5') bond is favored for C(3')-endo arabinosides, it is strongly stabilized for C(2')-endo arabinosides only in the presence of the intrasugar hydrogen bond O(2')-H ... O(5'). The net attractive electrostatic interactions between the phosphate and the base stabilizes the preferred conformations of 5'-arabinonucleotides also.", "contents": "Conformational analysis of arabinonucleosides and nucleotides. A comparison with the ribonucleosides and nucleotides. Conformations of arabino nucleosides and nucleotides have been analyzed by semiempirical energy calculations. It is found that the change in the configuration of the O(2')-hydroxyl group in arabinoses compared to riboses exerts significant influence on the conformational priorities of the glycosyl and the exocyclic C(4')-C(5') bond torsions. While the anti conformations for the bases are preferred, the anti in equilibrium or formed from syn interconversion is considerably hampered compared to ribosides due to large energy barrier. Further the preferred anti glycosyl torsions are shifted to higher values for C(3')-endo puckers and in ribosides. While the gauche+ conformation around the C(4')-C(5') bond is favored for C(3')-endo arabinosides, it is strongly stabilized for C(2')-endo arabinosides only in the presence of the intrasugar hydrogen bond O(2')-H ... O(5'). The net attractive electrostatic interactions between the phosphate and the base stabilizes the preferred conformations of 5'-arabinonucleotides also."}
{"id": "PMID:486483", "title": "Regulation of trypsin-like esteroprotease synthesis by 5 alpha-dihydrotestosterone and triiodothyronine in mouse submandibular gland.", "content": "The hormonal regulation of trypsin-like esteroprotease synthesis in mouse submandibular gland was studied at the isozyme level. Antiserum to a mixture of two purified esteroproteases precipitated all the esteroproteases in a crude extract of this gland. Measurement of incorporation of [3H]leucine showed that total esteroprotease synthesis was stimulated by both 5 alpha-dihydrotestosterone and triiodothyronine and that the two hormones had synergistic effects. The observed correlation between the increases of synthetic rate and specific activity of this enzyme suggests that the enzyme level is regulated mainly by the rate of enzyme synthesis. Newly synthesized esteroprotease-antibody complexes gave four peaks of radioactivity with esteroprotease activity and one peak without enzyme activity on isoelectric focusing in acrylamide gel containing 8 M urea. The radioactivities of these five peaks were increased similarly by the two hormones separately or in a combination. These results suggest that the actions of androgens and thyroid homrones in esteroprotease synthesis are indistinguishable at the isozyme level.", "contents": "Regulation of trypsin-like esteroprotease synthesis by 5 alpha-dihydrotestosterone and triiodothyronine in mouse submandibular gland. The hormonal regulation of trypsin-like esteroprotease synthesis in mouse submandibular gland was studied at the isozyme level. Antiserum to a mixture of two purified esteroproteases precipitated all the esteroproteases in a crude extract of this gland. Measurement of incorporation of [3H]leucine showed that total esteroprotease synthesis was stimulated by both 5 alpha-dihydrotestosterone and triiodothyronine and that the two hormones had synergistic effects. The observed correlation between the increases of synthetic rate and specific activity of this enzyme suggests that the enzyme level is regulated mainly by the rate of enzyme synthesis. Newly synthesized esteroprotease-antibody complexes gave four peaks of radioactivity with esteroprotease activity and one peak without enzyme activity on isoelectric focusing in acrylamide gel containing 8 M urea. The radioactivities of these five peaks were increased similarly by the two hormones separately or in a combination. These results suggest that the actions of androgens and thyroid homrones in esteroprotease synthesis are indistinguishable at the isozyme level."}
{"id": "PMID:486484", "title": "Contrasting denaturation maps of Xenopus laevis and Xenopus borealis mitochondrial DNAs.", "content": "Denaturation maps of mitochondrial DNAs of Xenopus laevis and Xenopus borealis are radically different from each other. This is in striking contrast to the invariant denaturation patterns previously recognized among mtDNAs of various Drosophila species, particularly, since the two toads may be even more closely related to each other than the Drosophila species.", "contents": "Contrasting denaturation maps of Xenopus laevis and Xenopus borealis mitochondrial DNAs. Denaturation maps of mitochondrial DNAs of Xenopus laevis and Xenopus borealis are radically different from each other. This is in striking contrast to the invariant denaturation patterns previously recognized among mtDNAs of various Drosophila species, particularly, since the two toads may be even more closely related to each other than the Drosophila species."}
{"id": "PMID:486485", "title": "Modification of lysyl residues of dihydrofolate reductase with 2,4-pentanedione.", "content": "Dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, EC 1.5.1.3) from an amethopterin-resistant strain of Lactobacillus casei was inactivated by 2,4-pentanedione. The inactivation appears to be due to the specific interaction of 2,4-pentanedione with lysyl residues. Inactivation is concomitant with with the modification of three lysyl residues. Both NADPH and dihydrofolate protect the enzyme against inactivation, suggesting that the critical residue(s) lies at or near their binding sites. Unlike native dihydrofolate reductase, 2,4-pentanedione-modified enzyme does not form binary complexes with either NADPH, dihydrofolate or amethopterin which are stable to gel filtration. Treatment of the modified enzyme with nucleophilic reagents such as hydroxylamine, failed to promote reactivation of the enzyme. Reactivation was achieved following gel filtration at pH 6.0 and was found to be dependent on the degree to which the enzyme was inactivated.", "contents": "Modification of lysyl residues of dihydrofolate reductase with 2,4-pentanedione. Dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, EC 1.5.1.3) from an amethopterin-resistant strain of Lactobacillus casei was inactivated by 2,4-pentanedione. The inactivation appears to be due to the specific interaction of 2,4-pentanedione with lysyl residues. Inactivation is concomitant with with the modification of three lysyl residues. Both NADPH and dihydrofolate protect the enzyme against inactivation, suggesting that the critical residue(s) lies at or near their binding sites. Unlike native dihydrofolate reductase, 2,4-pentanedione-modified enzyme does not form binary complexes with either NADPH, dihydrofolate or amethopterin which are stable to gel filtration. Treatment of the modified enzyme with nucleophilic reagents such as hydroxylamine, failed to promote reactivation of the enzyme. Reactivation was achieved following gel filtration at pH 6.0 and was found to be dependent on the degree to which the enzyme was inactivated."}
{"id": "PMID:486486", "title": "Serine hydroxymethylase. Specificity of bond cleaveage to form quinonoid intermediates and rate of holoenzyme formation.", "content": "L-Serine transhydroxymethylase (5,10-methylenetetrahydrofolate:glycine hydroxymethyltransferase, EC 2.1.2.1) a pyridoxal phosphate-dependent enzyme, has been obtained as a homogeneous preparation with a specific activity of 6.7 mumol benzaldehyde per minute at 30 degrees C at pH 7.5 in N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (Hepes) buffer, with DL-threo-beta-phenylserine as a substrate. This enzyme has been used to study the specificity of bond cleavage in forming quinonoid intermediates from DL and non-asymmetric amino acids. The ability of the generated quinonoids to react with formaldehyde and acetaldehyde has also been studied and evidence obtained for formation of the corresponding beta-hydroxymethyl and beta-hydroxyethyl amino acid derivaties. Apotranshydroxymethylase has been prepared and the rate of holoenzyme formation was found to be 0.52 min-1 by measuring Schiff base formation at 425 nm and 0.66 min-1 as determined from restoration of enzymic activity. A requirement for the presence of mercaptoethanol for complete reactivation was also established by these studies.", "contents": "Serine hydroxymethylase. Specificity of bond cleaveage to form quinonoid intermediates and rate of holoenzyme formation. L-Serine transhydroxymethylase (5,10-methylenetetrahydrofolate:glycine hydroxymethyltransferase, EC 2.1.2.1) a pyridoxal phosphate-dependent enzyme, has been obtained as a homogeneous preparation with a specific activity of 6.7 mumol benzaldehyde per minute at 30 degrees C at pH 7.5 in N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (Hepes) buffer, with DL-threo-beta-phenylserine as a substrate. This enzyme has been used to study the specificity of bond cleavage in forming quinonoid intermediates from DL and non-asymmetric amino acids. The ability of the generated quinonoids to react with formaldehyde and acetaldehyde has also been studied and evidence obtained for formation of the corresponding beta-hydroxymethyl and beta-hydroxyethyl amino acid derivaties. Apotranshydroxymethylase has been prepared and the rate of holoenzyme formation was found to be 0.52 min-1 by measuring Schiff base formation at 425 nm and 0.66 min-1 as determined from restoration of enzymic activity. A requirement for the presence of mercaptoethanol for complete reactivation was also established by these studies."}
{"id": "PMID:486487", "title": "Nucleoside deoxyribosyltransferase-II from Lactobacillus helveticus Substrate specificity studied. Pyrimidine bases as acceptors.", "content": "The nucleoside deoxyribosyltransferase (nucleoside:purine (pyrimidine) deoxyribosyltransferase, EC 2.4.2.6) fraction catalyzing specifically the transfer of the deoxyribosyl moiety from a purine (or a pyrimidine) to a pyrimidine (or a purine) exhibits a broad specificity for the acceptor base. With a pyrimidine base as the acceptor a -OH or -SH group adjacent to the N-1 atom is essential. A substituent on position 6 hinders the reaction. On positions 4 and 5 various substituent were found to influence the reaction rate and some of them give non-competent substrates. A few anomalous cases are also discussed in relation with the role of N-3. Deoxyribonucleosides can also be obtained with non-pyrimidine rings.", "contents": "Nucleoside deoxyribosyltransferase-II from Lactobacillus helveticus Substrate specificity studied. Pyrimidine bases as acceptors. The nucleoside deoxyribosyltransferase (nucleoside:purine (pyrimidine) deoxyribosyltransferase, EC 2.4.2.6) fraction catalyzing specifically the transfer of the deoxyribosyl moiety from a purine (or a pyrimidine) to a pyrimidine (or a purine) exhibits a broad specificity for the acceptor base. With a pyrimidine base as the acceptor a -OH or -SH group adjacent to the N-1 atom is essential. A substituent on position 6 hinders the reaction. On positions 4 and 5 various substituent were found to influence the reaction rate and some of them give non-competent substrates. A few anomalous cases are also discussed in relation with the role of N-3. Deoxyribonucleosides can also be obtained with non-pyrimidine rings."}
{"id": "PMID:486488", "title": "Detection of ectosiallyltransferase activity using whole cells. Correction of misleading results due to the release of intracellular CMP-N-acetylneuraminic acid.", "content": "An inhibitory effect due to broken cells is observed when sialyltransferase (CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) is measured with mixture of intact and homogenized lymphocytes. This intracellular inhibitory factor ib purified and characterized as CMP-N-acetylneuraminic acid (CMP-NeuNAc) by its behavior in various chromatographic and electrophoretic systems and by its susceptibility to CMP-NeuNAc hydrolase. This endogenous CMP-NeuNAc leads to an isotopic dilution of the exogenous labelled CMP-NeuNAc explaining the apparently lower activity of homogenate when compared to whole cells. Consequently, the radioactivity bound to acceptors may not be related to a known number of sialyl residues transferred, calling into question the validity of comparing the incorporation of [14C]NeuNAc by homogenate and whole cells in order to assign sialyltransferase activity to ectoenzyme. A new approach is developed to detect ectoglycosyltransferases with whole cells, taking into account that both intracellular enzymes and endogenous precursor may be introduced by the small percentage of broken cells.", "contents": "Detection of ectosiallyltransferase activity using whole cells. Correction of misleading results due to the release of intracellular CMP-N-acetylneuraminic acid. An inhibitory effect due to broken cells is observed when sialyltransferase (CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) is measured with mixture of intact and homogenized lymphocytes. This intracellular inhibitory factor ib purified and characterized as CMP-N-acetylneuraminic acid (CMP-NeuNAc) by its behavior in various chromatographic and electrophoretic systems and by its susceptibility to CMP-NeuNAc hydrolase. This endogenous CMP-NeuNAc leads to an isotopic dilution of the exogenous labelled CMP-NeuNAc explaining the apparently lower activity of homogenate when compared to whole cells. Consequently, the radioactivity bound to acceptors may not be related to a known number of sialyl residues transferred, calling into question the validity of comparing the incorporation of [14C]NeuNAc by homogenate and whole cells in order to assign sialyltransferase activity to ectoenzyme. A new approach is developed to detect ectoglycosyltransferases with whole cells, taking into account that both intracellular enzymes and endogenous precursor may be introduced by the small percentage of broken cells."}
{"id": "PMID:486489", "title": "The use of steady-state rate equations to analyse progress curve data.", "content": "Analysis of progress curves for enzyme-catalyzed reactions has been made by using a procedure that does not require the derivation of complex integrated rate equations. The method involves conversion of progress curve data to reaction velocities that are then fitted to the appropriate differential rate equation. Application of the procedure to data obtained for the reaction catalyzed by aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1), showed that the resulting values for the kinetic parameters agreed well with those obtained by conventional progress curve analysis (Duggleby, R.G. and Morrison, J.F. (1978) Biochim. Biophys. Acta 526, 398--409).", "contents": "The use of steady-state rate equations to analyse progress curve data. Analysis of progress curves for enzyme-catalyzed reactions has been made by using a procedure that does not require the derivation of complex integrated rate equations. The method involves conversion of progress curve data to reaction velocities that are then fitted to the appropriate differential rate equation. Application of the procedure to data obtained for the reaction catalyzed by aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1), showed that the resulting values for the kinetic parameters agreed well with those obtained by conventional progress curve analysis (Duggleby, R.G. and Morrison, J.F. (1978) Biochim. Biophys. Acta 526, 398--409)."}
{"id": "PMID:486490", "title": "Biomembrane cooperative enzymes. In vivo modulation of rat erythrocyte acetylcholinesterase by insulin in normal and diabetic conditions.", "content": "The present study investigated the effect of insulin in vivo on the changes in the cooperativity of a membrane-bound enzyme. The allosteric inhibition by F- of the erythrocyte membrane acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) was studied during intravenous glucose tolerance tests in control and alloxan-induced diabetic rats. In the former group, the value of n decreased from 1.6 to 1.0 whereas it remained about 1.6 in the latter groups. Intravenous injection of insulin (30 U/kg) decreased the values of n in both groups. It is suggested that the in vivo insulin action on membrane cooperative enzymes could also take place in insulin target cells.", "contents": "Biomembrane cooperative enzymes. In vivo modulation of rat erythrocyte acetylcholinesterase by insulin in normal and diabetic conditions. The present study investigated the effect of insulin in vivo on the changes in the cooperativity of a membrane-bound enzyme. The allosteric inhibition by F- of the erythrocyte membrane acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) was studied during intravenous glucose tolerance tests in control and alloxan-induced diabetic rats. In the former group, the value of n decreased from 1.6 to 1.0 whereas it remained about 1.6 in the latter groups. Intravenous injection of insulin (30 U/kg) decreased the values of n in both groups. It is suggested that the in vivo insulin action on membrane cooperative enzymes could also take place in insulin target cells."}
{"id": "PMID:486491", "title": "Distribution in spleen subcellular organelles of sialidase active towards natural sialogylcolipid and sialoglycoprotein substrates.", "content": "A procedure was devised for the preparation of enriched populations of subcellular organelles from homogenized bovine spleen. The fractions obtained were characterized for arylsulfatase, succinate dehydrogenase, UDPgalactosyltransferase and 5'-nucleotidase activities. The distribution of sialidase (acylneuraminyl hydrolase, EC 3.2.1.18) activity directed towards either endogenous substrate or exogenous ganglioside substrate suggests that it is enriched in the plasma membrane/microsomal fractions. Sialidase activity towards exogenous sialoglycoproteins, isolated from erythrocyte membrane, was enriched in the least dense of the plasma membrane/microsomal-containing fractions. The endogenous sialidase substrates were primarily the sialoglycolipids, hematoside and disialogangliosides. At the pH optimum, 3.8, and 37 degrees C, release of endogenous sialic acid was linear with time for 3 h. At the end of this time, 85% or more of the available endogenous substrate was hydrolyzed.", "contents": "Distribution in spleen subcellular organelles of sialidase active towards natural sialogylcolipid and sialoglycoprotein substrates. A procedure was devised for the preparation of enriched populations of subcellular organelles from homogenized bovine spleen. The fractions obtained were characterized for arylsulfatase, succinate dehydrogenase, UDPgalactosyltransferase and 5'-nucleotidase activities. The distribution of sialidase (acylneuraminyl hydrolase, EC 3.2.1.18) activity directed towards either endogenous substrate or exogenous ganglioside substrate suggests that it is enriched in the plasma membrane/microsomal fractions. Sialidase activity towards exogenous sialoglycoproteins, isolated from erythrocyte membrane, was enriched in the least dense of the plasma membrane/microsomal-containing fractions. The endogenous sialidase substrates were primarily the sialoglycolipids, hematoside and disialogangliosides. At the pH optimum, 3.8, and 37 degrees C, release of endogenous sialic acid was linear with time for 3 h. At the end of this time, 85% or more of the available endogenous substrate was hydrolyzed."}
{"id": "PMID:486492", "title": "Decarboxylation of ornithine and lysine in rat tissues.", "content": "The possibility that arginine and lysine might be decarboxylated by rat tissues was investigated. No evidence for decarboxylation of arginine could be found. Lysine decarbosylase (L-lysine carboxy-lyase, EC 4.1.1.18) activity producing CO2 and cadaverine was detected in extracts from rat ventral prostate, androgen-stimulated mouse kidney, regenerating rat liver and livers from rats pretreated with thioacetamide. These tissues all have high ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activities. Lysine and ornithine decarboxylase activities were lost to similar extents on inhibition of protein synthesis by cycloheximide and on exposure to alpha-difluoromethylornithine. A highly purified ornithine decarboxylase preparation was able to decarboxylate lysine and the ratio of ornithine to lysine decarboxylase activities was constant throughout purification. Kinetic studies of the purified preparation showed that the V for ornithine was about 4-fold greater than for lysine, but the Km for lysine (9 mM) was 100-times greater than that for ornithine (0.09 mM). These experiments indicate that all of the detectable lysine decarboxylase activity in rat and mouse tissues was due to the action of ornithine decarboxylase and that significant cadaverine production in vivo would occur only when ornithine decarboxylase activity is high and lysine concentrations substantially exceed those of ornithine.", "contents": "Decarboxylation of ornithine and lysine in rat tissues. The possibility that arginine and lysine might be decarboxylated by rat tissues was investigated. No evidence for decarboxylation of arginine could be found. Lysine decarbosylase (L-lysine carboxy-lyase, EC 4.1.1.18) activity producing CO2 and cadaverine was detected in extracts from rat ventral prostate, androgen-stimulated mouse kidney, regenerating rat liver and livers from rats pretreated with thioacetamide. These tissues all have high ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activities. Lysine and ornithine decarboxylase activities were lost to similar extents on inhibition of protein synthesis by cycloheximide and on exposure to alpha-difluoromethylornithine. A highly purified ornithine decarboxylase preparation was able to decarboxylate lysine and the ratio of ornithine to lysine decarboxylase activities was constant throughout purification. Kinetic studies of the purified preparation showed that the V for ornithine was about 4-fold greater than for lysine, but the Km for lysine (9 mM) was 100-times greater than that for ornithine (0.09 mM). These experiments indicate that all of the detectable lysine decarboxylase activity in rat and mouse tissues was due to the action of ornithine decarboxylase and that significant cadaverine production in vivo would occur only when ornithine decarboxylase activity is high and lysine concentrations substantially exceed those of ornithine."}
{"id": "PMID:486494", "title": "Purification of Thiobacillus denitrificans siroheme sulfite reductase and investigation of some molecular and catalytic properties.", "content": "A siroheme-containing sulfite reductase was isolated from Thiobacillus denitrificans, purified to an electrophoretically homogenous state, and investigated with regard to some of its molecular and catalytic properties. The enzyme was a tetramer with a molecular weight of 160 000, consisting of two types of subunits arranged to an alpha 2 beta 2-structure. The molecular weight of the alpha-subunit was 38 000, that of the beta-subunit 43 000. As prosthetic groups siroheme and Fe/S groupings could be detected. The absorption spectrum showed maxima at 273 nm, 393 nm, and 594 nm; the molar extinction coefficient at these wavelengths were 280, 181, and 60 . 10(3) cm2 . mmol-1, respectively. With reduced viologen dyes the enzyme reduced sulfite to sulfide, thiosulfate and trithionate. In many properties T. denitrificans sulfite reductase closely resembled desulfoviridin, the dissimilatory sulfite reductase of Dssulfovibrio species. It is proposed that the physiological function of this enzyme is not to reduce but rather to form sulfite from reduced sulfur compounds in the course of dissimilatory sulfur oxidation in T. denitrificans.", "contents": "Purification of Thiobacillus denitrificans siroheme sulfite reductase and investigation of some molecular and catalytic properties. A siroheme-containing sulfite reductase was isolated from Thiobacillus denitrificans, purified to an electrophoretically homogenous state, and investigated with regard to some of its molecular and catalytic properties. The enzyme was a tetramer with a molecular weight of 160 000, consisting of two types of subunits arranged to an alpha 2 beta 2-structure. The molecular weight of the alpha-subunit was 38 000, that of the beta-subunit 43 000. As prosthetic groups siroheme and Fe/S groupings could be detected. The absorption spectrum showed maxima at 273 nm, 393 nm, and 594 nm; the molar extinction coefficient at these wavelengths were 280, 181, and 60 . 10(3) cm2 . mmol-1, respectively. With reduced viologen dyes the enzyme reduced sulfite to sulfide, thiosulfate and trithionate. In many properties T. denitrificans sulfite reductase closely resembled desulfoviridin, the dissimilatory sulfite reductase of Dssulfovibrio species. It is proposed that the physiological function of this enzyme is not to reduce but rather to form sulfite from reduced sulfur compounds in the course of dissimilatory sulfur oxidation in T. denitrificans."}
{"id": "PMID:486495", "title": "A note on the synthesis of 5'-AMP ester of tris (hydroxymethyl)aminomethane by rat liver plasma membrane.", "content": "The alcohol-AMP synthesizine enzyme of rat liver plasma membrane also synthesizes the 5'-AMP ester of tris(hydroxymethyl)aminomethane as judged by the use of [alpha-32P] ATP and [U-14C] ATP. This synthetic process may decrease significantly the concentration of ATP during incubation.", "contents": "A note on the synthesis of 5'-AMP ester of tris (hydroxymethyl)aminomethane by rat liver plasma membrane. The alcohol-AMP synthesizine enzyme of rat liver plasma membrane also synthesizes the 5'-AMP ester of tris(hydroxymethyl)aminomethane as judged by the use of [alpha-32P] ATP and [U-14C] ATP. This synthetic process may decrease significantly the concentration of ATP during incubation."}
{"id": "PMID:486496", "title": "Observations on the kinetics of action of polyspermine-ribonuclease on poly(A).poly(U).", "content": "Starting from the observation that double-stranded ribonucleic acids are hydrolyzed more rapidly by bovine pancreatic ribonuclease that has been cross-linked to polyspermine, we have made an initial examination of the kinetics of the process. The addition of eight residues of the polyamine serves to strengthen the binding to poly(A).poly(U) 100-fold (Km changes from 2.7 . 10(-4) to 2.7 . 10(-6) M in total U) and to increase V for hydrolysis of the susceptible poly(U) strand from 2.5 to 16.2 delta A250 . min-1 per mg enzyme. There is evidence for inhibition by the RNAase-resistant poly(A) tracts in the substrate; free poly(A) shows a Ki of about 8 . 10(-6) M in total A.", "contents": "Observations on the kinetics of action of polyspermine-ribonuclease on poly(A).poly(U). Starting from the observation that double-stranded ribonucleic acids are hydrolyzed more rapidly by bovine pancreatic ribonuclease that has been cross-linked to polyspermine, we have made an initial examination of the kinetics of the process. The addition of eight residues of the polyamine serves to strengthen the binding to poly(A).poly(U) 100-fold (Km changes from 2.7 . 10(-4) to 2.7 . 10(-6) M in total U) and to increase V for hydrolysis of the susceptible poly(U) strand from 2.5 to 16.2 delta A250 . min-1 per mg enzyme. There is evidence for inhibition by the RNAase-resistant poly(A) tracts in the substrate; free poly(A) shows a Ki of about 8 . 10(-6) M in total A."}
{"id": "PMID:486497", "title": "4-Hydroxy-3-nitrophenylglyoxal. A chromophoric reagent for arginyl residues in proteins.", "content": "The chromophoric reagent, 4-hydroxy-3-nitrophenylglyoxal, is highly selective for the modification of arginine in aqueous solution at pH 7--9. The reagent also inactivates creatine kinase (ATP:creatine N-phosphotransferase, EC 2.7.3.2) in a manner analogous to that reported with phenylglyoxal.", "contents": "4-Hydroxy-3-nitrophenylglyoxal. A chromophoric reagent for arginyl residues in proteins. The chromophoric reagent, 4-hydroxy-3-nitrophenylglyoxal, is highly selective for the modification of arginine in aqueous solution at pH 7--9. The reagent also inactivates creatine kinase (ATP:creatine N-phosphotransferase, EC 2.7.3.2) in a manner analogous to that reported with phenylglyoxal."}
{"id": "PMID:486498", "title": "Purification and properties of alanine dehydrogenase from Halobacterium salinarium.", "content": "1. L-Alanine dehydrogenase (L-alanine:NAD+ oxidoreductase (deaminating), EC 1.4.1.1) was purified about 500-fold from Halobacterium salinarium. 2. The enzyme appears to be homogeneous in polyacrylamide gel electrophoresis. The apparent molecular weight is about 60 000. 3. Activity and stability of the enzyme are largely affected by different salts. Full activity of the NADH-dependent reductive amination of pyruvate occurs at 4.3 M NaCl. This activation can be achieved also by KCl and several other salts instead of NaCl. 4. The NAD+-dependent oxidative deamination of L-alanine occurs only in the presence of high concentrations of KCl. This reaction is not stimulated by NaCl. The Km values for the substrates NADH, pyruvate and NH+4 are also salt dependent. 5. The thermal stability of the enzyme is considerably higher in the presence of high concentrations of NaCl than in the presence of KCl. 6. The enzyme is completely inactivated by the removal of salt. Full reactivation is achieved by addition of salt in the presence of 2-mercaptoethanol. Inactivation proceeds about ten times faster than reactivation. The inactivation after the withdrawal of salt and the reactivation following the readdition of salt show a characteristic hysteresis loop.", "contents": "Purification and properties of alanine dehydrogenase from Halobacterium salinarium. 1. L-Alanine dehydrogenase (L-alanine:NAD+ oxidoreductase (deaminating), EC 1.4.1.1) was purified about 500-fold from Halobacterium salinarium. 2. The enzyme appears to be homogeneous in polyacrylamide gel electrophoresis. The apparent molecular weight is about 60 000. 3. Activity and stability of the enzyme are largely affected by different salts. Full activity of the NADH-dependent reductive amination of pyruvate occurs at 4.3 M NaCl. This activation can be achieved also by KCl and several other salts instead of NaCl. 4. The NAD+-dependent oxidative deamination of L-alanine occurs only in the presence of high concentrations of KCl. This reaction is not stimulated by NaCl. The Km values for the substrates NADH, pyruvate and NH+4 are also salt dependent. 5. The thermal stability of the enzyme is considerably higher in the presence of high concentrations of NaCl than in the presence of KCl. 6. The enzyme is completely inactivated by the removal of salt. Full reactivation is achieved by addition of salt in the presence of 2-mercaptoethanol. Inactivation proceeds about ten times faster than reactivation. The inactivation after the withdrawal of salt and the reactivation following the readdition of salt show a characteristic hysteresis loop."}
{"id": "PMID:486499", "title": "Effects of monovalent cations on AMP nucleosidase from Azotobacter vinelandii.", "content": "The effect of monovalent cations on the purified AMP nucleosidase (AMP phosphoribohydrolase, EC 3.2.2.4) from Azotobacter vinelandii was investigated. All the monovalent cations were activators of the enzyme: Rb+ and Cs+ were the most effective, followed by K+, Na+, NH4+ and Li+ in that order. The apparent Ka for MgATP and nH values (Hill's interaction coefficient) decreased from 0.9 to 0.1 mM, and from 4 to 1, respectively, with the increase in K+ concentration, suggesting that the cation effects are on MgATP binding rather than catalysis. Gel filtration studies have revealed that the enzyme forms a non-dissociable enzyme species with a Stokes radius of 6.0--6.2 nm in the presence of saturating concentrations of monovalent cations, which can be distinguished from the 5.5-nm enzyme species showing temperature-dependent dissociation of the molecule in sulfate or phosphate. These results suggest that these ligands affect the association of the subunits through changes in the environment of the hydrophobic side chains of the enzyme molecules.", "contents": "Effects of monovalent cations on AMP nucleosidase from Azotobacter vinelandii. The effect of monovalent cations on the purified AMP nucleosidase (AMP phosphoribohydrolase, EC 3.2.2.4) from Azotobacter vinelandii was investigated. All the monovalent cations were activators of the enzyme: Rb+ and Cs+ were the most effective, followed by K+, Na+, NH4+ and Li+ in that order. The apparent Ka for MgATP and nH values (Hill's interaction coefficient) decreased from 0.9 to 0.1 mM, and from 4 to 1, respectively, with the increase in K+ concentration, suggesting that the cation effects are on MgATP binding rather than catalysis. Gel filtration studies have revealed that the enzyme forms a non-dissociable enzyme species with a Stokes radius of 6.0--6.2 nm in the presence of saturating concentrations of monovalent cations, which can be distinguished from the 5.5-nm enzyme species showing temperature-dependent dissociation of the molecule in sulfate or phosphate. These results suggest that these ligands affect the association of the subunits through changes in the environment of the hydrophobic side chains of the enzyme molecules."}
{"id": "PMID:486500", "title": "Preparation and characterization, using high-performance liquid chromatography, of an enzyme forming glucosides of cytokinins.", "content": "Cytokinins can occur naturally as glycosides with beta-D-glucose as the sugar substituent. From radish (Raphanus sativus) cotyledons, an enzyme has been partly purified which synthesizes the 7-glucopyranoside of zeatin [6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine], a compound known to occur in this species. High-performance reverse-phase liquid chromatography was uniquely useful as the analytical procedure for quantitative study of the minute amounts of enzyme available. The enzyme uses UDPglucose as the source of the sugar residue. A large number of derivatives of purine are glucosylated, but adenine derivatives with an alkyl side chain at least three carbon atoms in length at position N6 are preferentially glucosylated. This corresponds to the structural features required for high cytokinin activity. The 7-glucoside of zeatin is known to be very weakly active in cytokinin bioassays. Hence, this enzyme, and others catalyzing the same reaction, have a role in the regulation of cytokinin activity.", "contents": "Preparation and characterization, using high-performance liquid chromatography, of an enzyme forming glucosides of cytokinins. Cytokinins can occur naturally as glycosides with beta-D-glucose as the sugar substituent. From radish (Raphanus sativus) cotyledons, an enzyme has been partly purified which synthesizes the 7-glucopyranoside of zeatin [6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine], a compound known to occur in this species. High-performance reverse-phase liquid chromatography was uniquely useful as the analytical procedure for quantitative study of the minute amounts of enzyme available. The enzyme uses UDPglucose as the source of the sugar residue. A large number of derivatives of purine are glucosylated, but adenine derivatives with an alkyl side chain at least three carbon atoms in length at position N6 are preferentially glucosylated. This corresponds to the structural features required for high cytokinin activity. The 7-glucoside of zeatin is known to be very weakly active in cytokinin bioassays. Hence, this enzyme, and others catalyzing the same reaction, have a role in the regulation of cytokinin activity."}
{"id": "PMID:486501", "title": "The role of zinc with special reference to the essential thiol groups in delta-aminolevulinic acid dehydratase of bovine liver.", "content": "delta-Aminolevulinic acid dehydratase (5-aminolevulinic acid hydro-lyase (adding 5-aminolevulinic acid and cyclizing), EC 4.2.1.24 purified from bovine liver in the presence of both SH-reducing reagent and zinc during the purification contained one zinc atom and eight SH groups/subunit. This preparation showed the full enzymatic activity even in the absence of thiol activator. It was found that two cysteine residues, one zinc atom and two histidine residues were involved in the active site. The enzyme was fullly active as long as two SH groups in the active site remained in the reduced form even in the absence of zinc. However, the enzymatic activity was completely lost, with a concomitant loss of bound zinc, upon oxidation of the SH groups to a disulfide bond, modification of SH groups with chemical reagents, or mercaptide formation by heavy metals. Thus, it is apparent that the activity depends on the essential SH groups. The zinc is not absolutely essential for the activity but may be required to prevent the essential SH groups from autooxidation by coordination. Binding experiments indicated that there was one binding site of zinc/subunit. Photooxidation of histidine residues diminished both enzymatic activity and bound zinc, suggesting that the histidine residues not only constituted the active site but also served as a possible ligand to zinc.", "contents": "The role of zinc with special reference to the essential thiol groups in delta-aminolevulinic acid dehydratase of bovine liver. delta-Aminolevulinic acid dehydratase (5-aminolevulinic acid hydro-lyase (adding 5-aminolevulinic acid and cyclizing), EC 4.2.1.24 purified from bovine liver in the presence of both SH-reducing reagent and zinc during the purification contained one zinc atom and eight SH groups/subunit. This preparation showed the full enzymatic activity even in the absence of thiol activator. It was found that two cysteine residues, one zinc atom and two histidine residues were involved in the active site. The enzyme was fullly active as long as two SH groups in the active site remained in the reduced form even in the absence of zinc. However, the enzymatic activity was completely lost, with a concomitant loss of bound zinc, upon oxidation of the SH groups to a disulfide bond, modification of SH groups with chemical reagents, or mercaptide formation by heavy metals. Thus, it is apparent that the activity depends on the essential SH groups. The zinc is not absolutely essential for the activity but may be required to prevent the essential SH groups from autooxidation by coordination. Binding experiments indicated that there was one binding site of zinc/subunit. Photooxidation of histidine residues diminished both enzymatic activity and bound zinc, suggesting that the histidine residues not only constituted the active site but also served as a possible ligand to zinc."}
{"id": "PMID:486502", "title": "Purification to homogeneity and some properties of L-phenylalanine ammonia-lyase of irradiated mustard (Sinapis alba L.) cotyledons.", "content": "1. Lyase (L-Phenylalanine ammonia-lyase, EC 4.3.1.5) from far-red light-irradiated mustard cotyledons was purified to a single protein using ammonium sulphate fractionation, column chromatography on L-phenylalanyl-Sepharose 4B and on Sephadex G-200, isoelectric focusing and polyacryalmide gel electrophoresis. 2. The enzyme constituted 0.01% of total cellular protein, did not catalyse the deamination of L-tyrosine, had a pH optimum of pH 8.6 and an isoelectric point of pH 5.6. 3. The sedimentation coefficient was estimated as 11.3 S, the Stokes' radius 4.25 nm, and the molecular weight 240 000 +/- 9000 (S.E.). 4. Electrophoresis on denaturing polyacrylamide gels gave a single stained protein band corresponding to a subunit molecular weight of 55 000 indicating a tetrameric structure of equal (or near-equal) size subunits. 5. Maximum velocity (V) for the purified lyase at 25 degrees C was 3.83--4.10 nkat. 1(-1) enzyme and Km value 0.151--0.154 mM. Negative cooperativity (Hill coefficient, n = 1.08) was not detected over the substrate concentration range tested. 6. A putative non-diffusible inhibitor isolated from dark-grown gherkin hypocotyls inhibited the homogeneously purified mustard lyase.", "contents": "Purification to homogeneity and some properties of L-phenylalanine ammonia-lyase of irradiated mustard (Sinapis alba L.) cotyledons. 1. Lyase (L-Phenylalanine ammonia-lyase, EC 4.3.1.5) from far-red light-irradiated mustard cotyledons was purified to a single protein using ammonium sulphate fractionation, column chromatography on L-phenylalanyl-Sepharose 4B and on Sephadex G-200, isoelectric focusing and polyacryalmide gel electrophoresis. 2. The enzyme constituted 0.01% of total cellular protein, did not catalyse the deamination of L-tyrosine, had a pH optimum of pH 8.6 and an isoelectric point of pH 5.6. 3. The sedimentation coefficient was estimated as 11.3 S, the Stokes' radius 4.25 nm, and the molecular weight 240 000 +/- 9000 (S.E.). 4. Electrophoresis on denaturing polyacrylamide gels gave a single stained protein band corresponding to a subunit molecular weight of 55 000 indicating a tetrameric structure of equal (or near-equal) size subunits. 5. Maximum velocity (V) for the purified lyase at 25 degrees C was 3.83--4.10 nkat. 1(-1) enzyme and Km value 0.151--0.154 mM. Negative cooperativity (Hill coefficient, n = 1.08) was not detected over the substrate concentration range tested. 6. A putative non-diffusible inhibitor isolated from dark-grown gherkin hypocotyls inhibited the homogeneously purified mustard lyase."}
{"id": "PMID:486503", "title": "Dihydropyrimidine amidohydrolase is a zinc metalloenzyme.", "content": "Bovine liver dihydropyrimidine amidohydrolase (EC 3.5.2.2) has been subjected to atomic absorption analysis. Three different preparations of homogeneous enzyme indicated that the enzyme contains 4.3 +/- 0.3 g atoms of Zn2+ per mol of enzyme or 1.1 g atoms of Zn2+ per subunit. No Co2+, Mn2+, Mg2+ or Cd2+ was detected. Exhaustive dialysis against either o-phenanthroline or EDTA did not reduce enzyme activity; however, prolonged incubation with dipicolinic acid resulted in inactivation which can be reversed by either Zn2+ or Co2+ but not Mg2+.", "contents": "Dihydropyrimidine amidohydrolase is a zinc metalloenzyme. Bovine liver dihydropyrimidine amidohydrolase (EC 3.5.2.2) has been subjected to atomic absorption analysis. Three different preparations of homogeneous enzyme indicated that the enzyme contains 4.3 +/- 0.3 g atoms of Zn2+ per mol of enzyme or 1.1 g atoms of Zn2+ per subunit. No Co2+, Mn2+, Mg2+ or Cd2+ was detected. Exhaustive dialysis against either o-phenanthroline or EDTA did not reduce enzyme activity; however, prolonged incubation with dipicolinic acid resulted in inactivation which can be reversed by either Zn2+ or Co2+ but not Mg2+."}
{"id": "PMID:486504", "title": "Behavior of N-methylated allopurinols and related 4-thioxopyrazolo [3,4-d]pyrimidines towards bovine milk xanthine oxidase.", "content": "1. All available N-mono- and N,N'-dimethylallopurinols and the corresponding 4-thioxo derivatives have been tested as substrates or inhibitors of bovine milk xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.2.3.2). 2. None of the compounds tested revealed any inhibitory activity towards the enzyme. 3. All compounds were resistant to enzymic oxidation, with the exception of 7-methylallopurinol and its 4-thioxo analog. Both these compounds were attacked at position 6. 7-Methylallopurinol was oxidised nearly ten times faster than the isomeric 3-methylhypoxanthine. 4. These observations can be explained by assuming that for attack at C-6, the enzyme must bind both to N-1 and N-2 in the pyrazole ring and causes tautomerisation, which places a double bond at position 5,6 in the pyrimidine ring. This activation process resembles the activation of hypoxanthine.", "contents": "Behavior of N-methylated allopurinols and related 4-thioxopyrazolo [3,4-d]pyrimidines towards bovine milk xanthine oxidase. 1. All available N-mono- and N,N'-dimethylallopurinols and the corresponding 4-thioxo derivatives have been tested as substrates or inhibitors of bovine milk xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.2.3.2). 2. None of the compounds tested revealed any inhibitory activity towards the enzyme. 3. All compounds were resistant to enzymic oxidation, with the exception of 7-methylallopurinol and its 4-thioxo analog. Both these compounds were attacked at position 6. 7-Methylallopurinol was oxidised nearly ten times faster than the isomeric 3-methylhypoxanthine. 4. These observations can be explained by assuming that for attack at C-6, the enzyme must bind both to N-1 and N-2 in the pyrazole ring and causes tautomerisation, which places a double bond at position 5,6 in the pyrimidine ring. This activation process resembles the activation of hypoxanthine."}
{"id": "PMID:486505", "title": "Properties of Pseudomonas AM1 primary-amine dehydrogenase immobilized on agarose.", "content": "1. The primary-amine dehydrogenase of Pseudomonas AM1 (primary amine:(acceptor) oxidoreductase (deaminating), EC 1.4.99.-) was purified by an improved method and covalently attached to cyanogen bromide-activated Sepharose 4B. The immobilized enzyme showed very little change in its sensitivity to heat and to inhibition by semicarbazide as compared with the soluble enzyme, but had enhanced stability at 0 degrees C. The pH optimum of the immobilized enzyme remained unchanged at pH 7.4. 2. A new type of spectrophotometric assay is described in which sedimentation of the immobilized enzyme in the cuvette is prevented by increasing the viscosity by the presence of 10% (w/w) polyethylene glycol (M1 20 000). Detailed kinetic analysis using this assay showed only insignificant differences in the Km values for n-butylamine and phenazine methosulphate between the soluble and Agarose-bound enzymes. The results are compared with those for other oxidoreductase enzymes immobilized on Sepharose.", "contents": "Properties of Pseudomonas AM1 primary-amine dehydrogenase immobilized on agarose. 1. The primary-amine dehydrogenase of Pseudomonas AM1 (primary amine:(acceptor) oxidoreductase (deaminating), EC 1.4.99.-) was purified by an improved method and covalently attached to cyanogen bromide-activated Sepharose 4B. The immobilized enzyme showed very little change in its sensitivity to heat and to inhibition by semicarbazide as compared with the soluble enzyme, but had enhanced stability at 0 degrees C. The pH optimum of the immobilized enzyme remained unchanged at pH 7.4. 2. A new type of spectrophotometric assay is described in which sedimentation of the immobilized enzyme in the cuvette is prevented by increasing the viscosity by the presence of 10% (w/w) polyethylene glycol (M1 20 000). Detailed kinetic analysis using this assay showed only insignificant differences in the Km values for n-butylamine and phenazine methosulphate between the soluble and Agarose-bound enzymes. The results are compared with those for other oxidoreductase enzymes immobilized on Sepharose."}
{"id": "PMID:486506", "title": "Isolation and characterization of a butyrylesterase from human erythrocytes.", "content": "Human erythrocytes contain a butyrylesterase which, judging from the ease with which it can be solubilized, is present in the cytoplasm of these cells. This enzyme has been isolated and a number of its properties characterized. The purified enzyme hydrolyzed butyryl esters with both a lower Km and higher V than is seen with esters containing longer or shorter acyl groups. It has a molecular weight of 320 000 and an isoelectric point of 4.1. This low isoelectric point is apparently a result of the relatively high content of glutamic and aspartic acids. The stability of the isolated butyrylesterase has been examined under a number of different conditions. The enzyme is inhibited by low concentrations of Hg2+, Cd2+, Zn2+ and the organophosphorus compound Mipafox, but is insensitive to eserine. The properties of this butyrylesterase, including its ability to hydrolyze thiocholine esters at a relatively rapid rate (albeit with a high Km), are a mixture of those expected for an arylesterase and a cholinesterase.", "contents": "Isolation and characterization of a butyrylesterase from human erythrocytes. Human erythrocytes contain a butyrylesterase which, judging from the ease with which it can be solubilized, is present in the cytoplasm of these cells. This enzyme has been isolated and a number of its properties characterized. The purified enzyme hydrolyzed butyryl esters with both a lower Km and higher V than is seen with esters containing longer or shorter acyl groups. It has a molecular weight of 320 000 and an isoelectric point of 4.1. This low isoelectric point is apparently a result of the relatively high content of glutamic and aspartic acids. The stability of the isolated butyrylesterase has been examined under a number of different conditions. The enzyme is inhibited by low concentrations of Hg2+, Cd2+, Zn2+ and the organophosphorus compound Mipafox, but is insensitive to eserine. The properties of this butyrylesterase, including its ability to hydrolyze thiocholine esters at a relatively rapid rate (albeit with a high Km), are a mixture of those expected for an arylesterase and a cholinesterase."}
{"id": "PMID:486507", "title": "Purification and properties of human serum esterase.", "content": "Human serum esterase was purified by affinity column chromatography on a column of covalently linked p-trimethylammoniumanilinium dichloride to Sepharose 4B. The purified preparation hydrolysed both benzoylcholine and tributyrin. p-Trimethylammoniumanilinium dichloride inhibited non-competitively the hydrolysis of tributyrin and inhibited competitively the splitting of benzoylcholine. The Km value was 0.62 . 10(-3) M for tributyrin and 0.4 . 10(-3) M for benzoylcholine. Antiserum to this purified esterase was prepared in rabbit and it was found that the antiserum did not inhibit esterase activities of human liver, muscle and adipose tissue, although it could inhibit completely the esterase activities of human serum.", "contents": "Purification and properties of human serum esterase. Human serum esterase was purified by affinity column chromatography on a column of covalently linked p-trimethylammoniumanilinium dichloride to Sepharose 4B. The purified preparation hydrolysed both benzoylcholine and tributyrin. p-Trimethylammoniumanilinium dichloride inhibited non-competitively the hydrolysis of tributyrin and inhibited competitively the splitting of benzoylcholine. The Km value was 0.62 . 10(-3) M for tributyrin and 0.4 . 10(-3) M for benzoylcholine. Antiserum to this purified esterase was prepared in rabbit and it was found that the antiserum did not inhibit esterase activities of human liver, muscle and adipose tissue, although it could inhibit completely the esterase activities of human serum."}
{"id": "PMID:486509", "title": "Thermal stability of fatty acid-serum albumin complexes studied by differential scanning calorimetry.", "content": "Differential scanning calorimetry has been used to study the thermal stability of bovine serum albumin as affected by binding of fatty acids (lauric acid and stearic acid) and sodium dodecyl sulfate (SDS). All the ligands stabilized the protein molecules in a similar manner, but to different levels. A maximum increase in denaturation temperature of 30 degrees C was obtained with lauric acid. The thermograms indicate the presence of several ligand-albumin complexes having different heat stabilities. Variations in pH in 0.9% NaCl affected the heat stability of both ligand-poor and ligand-rich albumin, the former being more sensitive to variations in pH within the physiological range. Variations in NaCl concentration affected the thermal stabilities at neutral pH, expecially at low salt concentrations. While ligand-rich albumin was somewhat destabilized by increasing NaCl concentrations, ligand-poor albumin was strongly stabilized. The potential use of differential scanning calorimetry in ligand-albumin research is discussed.", "contents": "Thermal stability of fatty acid-serum albumin complexes studied by differential scanning calorimetry. Differential scanning calorimetry has been used to study the thermal stability of bovine serum albumin as affected by binding of fatty acids (lauric acid and stearic acid) and sodium dodecyl sulfate (SDS). All the ligands stabilized the protein molecules in a similar manner, but to different levels. A maximum increase in denaturation temperature of 30 degrees C was obtained with lauric acid. The thermograms indicate the presence of several ligand-albumin complexes having different heat stabilities. Variations in pH in 0.9% NaCl affected the heat stability of both ligand-poor and ligand-rich albumin, the former being more sensitive to variations in pH within the physiological range. Variations in NaCl concentration affected the thermal stabilities at neutral pH, expecially at low salt concentrations. While ligand-rich albumin was somewhat destabilized by increasing NaCl concentrations, ligand-poor albumin was strongly stabilized. The potential use of differential scanning calorimetry in ligand-albumin research is discussed."}
{"id": "PMID:486510", "title": "Glucocorticoid induction of pulmonary maturation in the rabbit fetus. The effect of maternal injection of betamethasone on the activity of enzymes in fetal lung.", "content": "1. Maternal administration of betamethasone (0.2 mg/kg) on day 25 or 26 of gestation produced a significant decrease in the lung/body weight ratio of the rabbit fetuses within 24 h. 2. The incorporation of [14C]choline but not [14C]ethanolamine into the lipids of fetal lung slices was significantly increased, indicating that there was a specific effect on phosphatidylcholine synthesis. 3. The activities of a number of marker enzymes for subcellular organelles were elevated, especially in the lungs of fetuses delivered on day 26. The increases in monoamine oxidase (mitochondrial outer membrane), beta-glycerophosphatase and aqueously dispersed phosphatidic acid-dependent phosphatidic acid phosphohydrolase (lysosomal) activities were significant. 4. Although the activity of cholinephosphotransferase was not affected by glucocorticoid treatment, the activities of glycerol-3-phosphate phosphatidyltransferase and the activities of two enzymes in the auxiliary pathways for the production of disaturated phosphatidylcholine (lysophosphatidylcholine:lysophosphatidylcholine transacylase and lysophosphatidylcholine:acyl-CoA acyl-transferase) were significantly increased. 5. Membrane-bound phosphatidic acid-dependent phosphatidic acid phosphohydrolase activity was elevated to a lesser extent than the aqueously dispersed phosphatidate-dependent activity and this increase was not significant. 6. The incorporation of E135S]methionine into protein by slices of fetal lung was significantly reduced after maternal treatment with betamethosone. 7. These results are consistent with the general view that glucocorticoids can induce pulmonary maturation and surfactant production in the rabbit fetus but indicate that some of the former hypotheses regarding the mechanism by which lipid synthesis is accelerated must be reevaluated.", "contents": "Glucocorticoid induction of pulmonary maturation in the rabbit fetus. The effect of maternal injection of betamethasone on the activity of enzymes in fetal lung. 1. Maternal administration of betamethasone (0.2 mg/kg) on day 25 or 26 of gestation produced a significant decrease in the lung/body weight ratio of the rabbit fetuses within 24 h. 2. The incorporation of [14C]choline but not [14C]ethanolamine into the lipids of fetal lung slices was significantly increased, indicating that there was a specific effect on phosphatidylcholine synthesis. 3. The activities of a number of marker enzymes for subcellular organelles were elevated, especially in the lungs of fetuses delivered on day 26. The increases in monoamine oxidase (mitochondrial outer membrane), beta-glycerophosphatase and aqueously dispersed phosphatidic acid-dependent phosphatidic acid phosphohydrolase (lysosomal) activities were significant. 4. Although the activity of cholinephosphotransferase was not affected by glucocorticoid treatment, the activities of glycerol-3-phosphate phosphatidyltransferase and the activities of two enzymes in the auxiliary pathways for the production of disaturated phosphatidylcholine (lysophosphatidylcholine:lysophosphatidylcholine transacylase and lysophosphatidylcholine:acyl-CoA acyl-transferase) were significantly increased. 5. Membrane-bound phosphatidic acid-dependent phosphatidic acid phosphohydrolase activity was elevated to a lesser extent than the aqueously dispersed phosphatidate-dependent activity and this increase was not significant. 6. The incorporation of E135S]methionine into protein by slices of fetal lung was significantly reduced after maternal treatment with betamethosone. 7. These results are consistent with the general view that glucocorticoids can induce pulmonary maturation and surfactant production in the rabbit fetus but indicate that some of the former hypotheses regarding the mechanism by which lipid synthesis is accelerated must be reevaluated."}
{"id": "PMID:486511", "title": "Occurrence of two 3-hydroxyacyl-CoA dehydrogenases in rat liver.", "content": "3-Hydroxyacyl-CoA dehydrogenase was assayed for acetoacetyl pantetheine-reducing and acetoacetyl-CoA reducing activities in rat liver homegenates. Two isoenzymes of the enzyme, types I and II, were distinguished by the following procedures: trypsin treatment, heat treatment, CM-cellulose chromatography, antibody titration, and sucrose density gradient centrifugation of the light mitochondrial fraction. Type I enzyme was localized in mitochondria, and catalyzed the reduction of both acetoacetyl pantetheine and acetoacetyl-CoA. Type II enzyme was found mainly in peroxisomes, accompanied by a low activity in mitochondria or some other organelles, and was active with acetoacetyl-CoA but not with aceto acetylpantetheine. Both isozymes were induced by the administration to the rats of di-(2-ethylhexyl)phthalate, which enhances the peroxisomal beta-oxidation activity, but the extent of the induction of type II enzyme was much higher than that of type I enzyme. The activity of the former was found only in diethylhexylphthalate-treated rats.", "contents": "Occurrence of two 3-hydroxyacyl-CoA dehydrogenases in rat liver. 3-Hydroxyacyl-CoA dehydrogenase was assayed for acetoacetyl pantetheine-reducing and acetoacetyl-CoA reducing activities in rat liver homegenates. Two isoenzymes of the enzyme, types I and II, were distinguished by the following procedures: trypsin treatment, heat treatment, CM-cellulose chromatography, antibody titration, and sucrose density gradient centrifugation of the light mitochondrial fraction. Type I enzyme was localized in mitochondria, and catalyzed the reduction of both acetoacetyl pantetheine and acetoacetyl-CoA. Type II enzyme was found mainly in peroxisomes, accompanied by a low activity in mitochondria or some other organelles, and was active with acetoacetyl-CoA but not with aceto acetylpantetheine. Both isozymes were induced by the administration to the rats of di-(2-ethylhexyl)phthalate, which enhances the peroxisomal beta-oxidation activity, but the extent of the induction of type II enzyme was much higher than that of type I enzyme. The activity of the former was found only in diethylhexylphthalate-treated rats."}
{"id": "PMID:486512", "title": "Kinetics of the in vivo labeling of the acyl groups of rabbit lung phosphatidylcholine and disaturated phosphatidylcholine.", "content": "The kinetics of labeling of lung phosphatidylcholine and disaturated phosphatidylcholine were studied for periods from 0.75--120 min following intravenous injection of radiolabeled palmitic acid and choline into 3-day-old rabbits. The labeled palmitic acid was cleared rapidly from plasma, and rapidly appeared with identical incorporation kinetics in both phosphatidylcholine and disaturated phosphatidylcholine. The 2-acyl positions of both phosphatidylcholine and disaturated phosphatidylcholine were labeled preferentially soon after [14C]palmitic acid injection. The specific activities of palmitic acid in the 2-acyl positions of phosphatidylcholine and disaturated phosphatidylcholine 0.75 min after injection of labeled palmitic acid were 3.4 and 1.9 times, respectively, the specific activities of palmitic acid in the 1-acyl positions. By 120 min the label had randomized between the 1-acyl and 2-acyl positions, and the kinetics of that randomization were defined for both phosphatidylcholine and disaturated phosphatidylcholine. Choline did not pulse label lung phosphatidylcholine or disaturated phosphatidylcholine. The choline label appeared with equal specific activities in both phosphatidylcholine and disaturated phosphatidylcholine. Thus no analysis of the de novo synthesized product via the CDP-choline pathway was possible.", "contents": "Kinetics of the in vivo labeling of the acyl groups of rabbit lung phosphatidylcholine and disaturated phosphatidylcholine. The kinetics of labeling of lung phosphatidylcholine and disaturated phosphatidylcholine were studied for periods from 0.75--120 min following intravenous injection of radiolabeled palmitic acid and choline into 3-day-old rabbits. The labeled palmitic acid was cleared rapidly from plasma, and rapidly appeared with identical incorporation kinetics in both phosphatidylcholine and disaturated phosphatidylcholine. The 2-acyl positions of both phosphatidylcholine and disaturated phosphatidylcholine were labeled preferentially soon after [14C]palmitic acid injection. The specific activities of palmitic acid in the 2-acyl positions of phosphatidylcholine and disaturated phosphatidylcholine 0.75 min after injection of labeled palmitic acid were 3.4 and 1.9 times, respectively, the specific activities of palmitic acid in the 1-acyl positions. By 120 min the label had randomized between the 1-acyl and 2-acyl positions, and the kinetics of that randomization were defined for both phosphatidylcholine and disaturated phosphatidylcholine. Choline did not pulse label lung phosphatidylcholine or disaturated phosphatidylcholine. The choline label appeared with equal specific activities in both phosphatidylcholine and disaturated phosphatidylcholine. Thus no analysis of the de novo synthesized product via the CDP-choline pathway was possible."}
{"id": "PMID:486513", "title": "The characteristics and metabolism of a genetically hypercholesterolemic strain of rats (RICO).", "content": "A genetically hypercholesterolemic strain of rats was selectively bred, starting from an ordinary albino mutant of Rattus norvegicus. The new strain was given the designation RICO, standing for rats with increased cholesterol. In these animals, hypercholesterolemia is established, in both sexes, one day after weaning, and it increases progressively thereafter. It is due to elevated concentrations of LDL- and HDL-cholesterol. As in the ordinary rat, the HDL fraction makes up the main part of the serum cholesterol in the RICO rat. Metabolic studies revealed that in the RICO strain the overall rate of hepatic cholesterol synthesis is accelerated, as a result of higher than normal activity of 3-hydroxy-3-methylglutaryl-CoA reductase. The activity of cholesterol-7 alpha-hydroxylase is decreased in RICO rats, indicating a lower than normal rate of cholesterol catabolism. No difference was found between RICO and ordinary rats with respect to fecal excretion of bile acids and cholesterol.", "contents": "The characteristics and metabolism of a genetically hypercholesterolemic strain of rats (RICO). A genetically hypercholesterolemic strain of rats was selectively bred, starting from an ordinary albino mutant of Rattus norvegicus. The new strain was given the designation RICO, standing for rats with increased cholesterol. In these animals, hypercholesterolemia is established, in both sexes, one day after weaning, and it increases progressively thereafter. It is due to elevated concentrations of LDL- and HDL-cholesterol. As in the ordinary rat, the HDL fraction makes up the main part of the serum cholesterol in the RICO rat. Metabolic studies revealed that in the RICO strain the overall rate of hepatic cholesterol synthesis is accelerated, as a result of higher than normal activity of 3-hydroxy-3-methylglutaryl-CoA reductase. The activity of cholesterol-7 alpha-hydroxylase is decreased in RICO rats, indicating a lower than normal rate of cholesterol catabolism. No difference was found between RICO and ordinary rats with respect to fecal excretion of bile acids and cholesterol."}
{"id": "PMID:486514", "title": "Adrenal hydroxylations in genetically obese and hypertensive rats.", "content": "The separate steps in the formation of aldosterone from cholesterol were studied in a strain of spontaneously hypertensive rats in which phenotypic obesity is inherited as a recessive trait (Koletsky rats). The obese and hypertensive state had little or no effect on side-chain cleavage of cholesterol, formation of progesterone from pregnenolone or 21-hydroxylation. Mitochondrial 18-hydroxylation of endogenous and exogenous corticosterone, however, as well as 18- and 11 beta-hydroxylation of deoxycorticosterone, were increased in obese hypertensive rats, both when compared with non-obese hypertensive siblings and when compared with healthy Sprague-Dawley rats. 18-Hydroxylation of corticosterone was increased more than 18-hydroxylation of deoxycorticosterone. In non-obese hypertensive rats, the adrenal content of mitochondrial cytochrome P-450 was lower than that in obese hypertensive rats but higher than that in rats of the conventional Sprague-Dawley strain. The results are discussed with respect to possible heterogeneity of adrenal cytochrome P-450 and to possible explanations for the changes observed.", "contents": "Adrenal hydroxylations in genetically obese and hypertensive rats. The separate steps in the formation of aldosterone from cholesterol were studied in a strain of spontaneously hypertensive rats in which phenotypic obesity is inherited as a recessive trait (Koletsky rats). The obese and hypertensive state had little or no effect on side-chain cleavage of cholesterol, formation of progesterone from pregnenolone or 21-hydroxylation. Mitochondrial 18-hydroxylation of endogenous and exogenous corticosterone, however, as well as 18- and 11 beta-hydroxylation of deoxycorticosterone, were increased in obese hypertensive rats, both when compared with non-obese hypertensive siblings and when compared with healthy Sprague-Dawley rats. 18-Hydroxylation of corticosterone was increased more than 18-hydroxylation of deoxycorticosterone. In non-obese hypertensive rats, the adrenal content of mitochondrial cytochrome P-450 was lower than that in obese hypertensive rats but higher than that in rats of the conventional Sprague-Dawley strain. The results are discussed with respect to possible heterogeneity of adrenal cytochrome P-450 and to possible explanations for the changes observed."}
{"id": "PMID:486515", "title": "Effect of in vitro aging on 6-ketoprostaglandin F1 alpha-producing activity in cultured human diploid lung fibroblasts.", "content": "Prostaglandin synthesis in human diploid fibroblasts was studied by incubating [14C]-arachidonic acid with cell homogenates. The majority of prostaglandins produced in young cells was 6-ketoprostaglandin F1 alpha. The 6-ketoprostaglandin F1 alpha-producing activity of cultures declined with in vitro aging, and was almost undetectable at the senescent stage, while total production of thromboxane B2, prostaglandin F2 alpha and prostaglandin E2-like metabolites increased with in vitro aging.", "contents": "Effect of in vitro aging on 6-ketoprostaglandin F1 alpha-producing activity in cultured human diploid lung fibroblasts. Prostaglandin synthesis in human diploid fibroblasts was studied by incubating [14C]-arachidonic acid with cell homogenates. The majority of prostaglandins produced in young cells was 6-ketoprostaglandin F1 alpha. The 6-ketoprostaglandin F1 alpha-producing activity of cultures declined with in vitro aging, and was almost undetectable at the senescent stage, while total production of thromboxane B2, prostaglandin F2 alpha and prostaglandin E2-like metabolites increased with in vitro aging."}
{"id": "PMID:486516", "title": "Retention of phospholipid acyl groups is not characteristic of neoplastic cells in culture.", "content": "Phospholipid acyl group retention of normal and mouse sarcoma virus transformed cell lines has been examined. The cells were fed [1-14C]oleate for 24 h and then grown to non-radioactive medium for 4 days. Both the transformed rat and mouse cell lines continued to release 14C-labeled fatty acids and phospholipids into the culture medium similarly to the uninfected cells, and showed shifts in 14C between phospholipid subclasses. Thus, the phospholipid acyl group stability reported for L cells is not characteristic of oncogenic cells in culture.", "contents": "Retention of phospholipid acyl groups is not characteristic of neoplastic cells in culture. Phospholipid acyl group retention of normal and mouse sarcoma virus transformed cell lines has been examined. The cells were fed [1-14C]oleate for 24 h and then grown to non-radioactive medium for 4 days. Both the transformed rat and mouse cell lines continued to release 14C-labeled fatty acids and phospholipids into the culture medium similarly to the uninfected cells, and showed shifts in 14C between phospholipid subclasses. Thus, the phospholipid acyl group stability reported for L cells is not characteristic of oncogenic cells in culture."}
{"id": "PMID:486517", "title": "In vitro lipid metabolism in the rat pancreas. I. Basal lipid metabolism.", "content": "1. The in vitro basal lipid metabolism of rat pancreatic fragments was compared with that in adipose tissue fragments and liver slices. 2. [1-14C]Acetate added to the media was mostly incorporated into palmitic acid and to a lesser extent into oleic acid. In addition, pancreatic tissue exhibited a marked capacity for elongation of polyunsaturated fatty acids by [1-14C]acetate and resulting desaturation when compared to adipose tissue and liver. 3. Data obtained in the presence of [U-14C]glucose, [1-14C]palmitate and 3H20 indicate that acetyl-CoA derived from glucose and from beta-oxidation of fatty acids contributed to de novo lipogenesis. 4. Oxidation of [1-14C]palmitic acid was 9-13 times higher in the pancreas than in adipose tissue or liver when expressed on a wet weight basis. 5. The fatty acid moiety of pancreatic glycerolipids could be derived from de novo synthesis, fatty acids added to the medium, or from fatty acids formed from the hydrolysis of endogenous lipids. The glycerol moiety could be derived either from glucose, or directly from glycerol through participation of glycerol kinase.", "contents": "In vitro lipid metabolism in the rat pancreas. I. Basal lipid metabolism. 1. The in vitro basal lipid metabolism of rat pancreatic fragments was compared with that in adipose tissue fragments and liver slices. 2. [1-14C]Acetate added to the media was mostly incorporated into palmitic acid and to a lesser extent into oleic acid. In addition, pancreatic tissue exhibited a marked capacity for elongation of polyunsaturated fatty acids by [1-14C]acetate and resulting desaturation when compared to adipose tissue and liver. 3. Data obtained in the presence of [U-14C]glucose, [1-14C]palmitate and 3H20 indicate that acetyl-CoA derived from glucose and from beta-oxidation of fatty acids contributed to de novo lipogenesis. 4. Oxidation of [1-14C]palmitic acid was 9-13 times higher in the pancreas than in adipose tissue or liver when expressed on a wet weight basis. 5. The fatty acid moiety of pancreatic glycerolipids could be derived from de novo synthesis, fatty acids added to the medium, or from fatty acids formed from the hydrolysis of endogenous lipids. The glycerol moiety could be derived either from glucose, or directly from glycerol through participation of glycerol kinase."}
{"id": "PMID:486518", "title": "In vitro lipid metabolism in the rat pancreas. II. Effects of secretagogues on fatty acid metabolism, net lipolysis and ATP levels.", "content": "1. The concentration of carbamylcholine, bombesin, pancreozymin, pentagastrin and secretin evoking a similar 4--5-fold maximal increase in amylase secretion from rat pancreatic fragments were 3.10(-6), 10(-7), 10(-8), 3.10(-6), and 3.10(-6) M, respectively. The maximal concentration of vasoactive intestinal peptide tested (3.10(-6) M) increased amylase secretion by 250%. The six secretagogues could be separated into two groups according to their effects on lipid metabolism and ATP levels. 2. When used at their optimal concentrations, carbamylcholine, bombesin, pancreozymin, and pentagastrin lowered pancreatic ATP levels by 18-26% and increased net release of free fatty acids by 68-105%. 3. The effects of 3.10(-6) M carbamylcholine and 10(-8) M pancreozymin on the metabolism of 3H2O, D-[U-14C]glucose and [1-14C]acetate were similar; the incorporation of radioactivity in the fatty acid moiety of glycerolipids decreased by 20--50% whereas the incorporation of 3H from 3H2O and of 14C from [U-14C]glucose increased by 20--35% in the glycerol moiety. In addition, the oxidation of [U-14C]glucose, [1-14C]acetate and [1-14C]palmitate to 14CO2 increased by 15--32% while the esterification of [1-14C]palmitate, [1-14C]-linoleate, and [1-14C]arachidonate was inhibited by 14--23%. The spectrum of fatty acids labeled with [1-14C]acetate indicated an inhibition of the malonic acid pathway whereas the elongation of polyenoic fatty acids was unaltered.", "contents": "In vitro lipid metabolism in the rat pancreas. II. Effects of secretagogues on fatty acid metabolism, net lipolysis and ATP levels. 1. The concentration of carbamylcholine, bombesin, pancreozymin, pentagastrin and secretin evoking a similar 4--5-fold maximal increase in amylase secretion from rat pancreatic fragments were 3.10(-6), 10(-7), 10(-8), 3.10(-6), and 3.10(-6) M, respectively. The maximal concentration of vasoactive intestinal peptide tested (3.10(-6) M) increased amylase secretion by 250%. The six secretagogues could be separated into two groups according to their effects on lipid metabolism and ATP levels. 2. When used at their optimal concentrations, carbamylcholine, bombesin, pancreozymin, and pentagastrin lowered pancreatic ATP levels by 18-26% and increased net release of free fatty acids by 68-105%. 3. The effects of 3.10(-6) M carbamylcholine and 10(-8) M pancreozymin on the metabolism of 3H2O, D-[U-14C]glucose and [1-14C]acetate were similar; the incorporation of radioactivity in the fatty acid moiety of glycerolipids decreased by 20--50% whereas the incorporation of 3H from 3H2O and of 14C from [U-14C]glucose increased by 20--35% in the glycerol moiety. In addition, the oxidation of [U-14C]glucose, [1-14C]acetate and [1-14C]palmitate to 14CO2 increased by 15--32% while the esterification of [1-14C]palmitate, [1-14C]-linoleate, and [1-14C]arachidonate was inhibited by 14--23%. The spectrum of fatty acids labeled with [1-14C]acetate indicated an inhibition of the malonic acid pathway whereas the elongation of polyenoic fatty acids was unaltered."}
{"id": "PMID:486519", "title": "An unusual lipid in the human pathogenic fungus Epidermophyton floccosum.", "content": "The dermatophyte Epidermophyton floccosum contains an unusual lipid characterized as 1(3),2-diacylglyceryl-3(1)-O-4'-(N,N,N-trimethyl)homoserine. Its concentration in E. floccosum is approx. 15% of the polar lipids. The role of this lipid in the biosynthesis of polar lipids and fatty acids is not yet known. However, it is interesting from a chemotaxonomic viewpoint that the lipids from two other genera of dermatophyte, Microsporum cookei and Trichophyton rubrum do not contain this novel lipid.", "contents": "An unusual lipid in the human pathogenic fungus Epidermophyton floccosum. The dermatophyte Epidermophyton floccosum contains an unusual lipid characterized as 1(3),2-diacylglyceryl-3(1)-O-4'-(N,N,N-trimethyl)homoserine. Its concentration in E. floccosum is approx. 15% of the polar lipids. The role of this lipid in the biosynthesis of polar lipids and fatty acids is not yet known. However, it is interesting from a chemotaxonomic viewpoint that the lipids from two other genera of dermatophyte, Microsporum cookei and Trichophyton rubrum do not contain this novel lipid."}
{"id": "PMID:486520", "title": "Effect of hydrazine exposure on hepatic triacylglycerol biosynthesis.", "content": "Acute hydrazine exposure elevated rat liver triacylglycerol content and produced a rapid rise in triacylglycerol production from sn-[1,3-14C]glycerol 3-phosphate by liver homogenate and microsomal fractions. Hydrazine treatment also increased the incorporation of [1,3-14C]glycerol into hepatic triacylglycerol by the intact animal. Homogenates of hepatocyte monolayers exposed to hydrazine in vitro also exhibited an increased capacity to form triacylglycerol from sn-[1,3-14C]glycerol 3-phosphate. Hydrazine-dependent increases in hepatic triacylglycerol production measured in vitro correlated well with an increase in microsomal phosphatidate phosphohydrolase (EC 3.1.3.4) activity. Therefore, the fatty liver associated with hydrazine exposure may be explained in part by a rise in the enzymatic capacity of hepatic triacylglycerol biosynthesis.", "contents": "Effect of hydrazine exposure on hepatic triacylglycerol biosynthesis. Acute hydrazine exposure elevated rat liver triacylglycerol content and produced a rapid rise in triacylglycerol production from sn-[1,3-14C]glycerol 3-phosphate by liver homogenate and microsomal fractions. Hydrazine treatment also increased the incorporation of [1,3-14C]glycerol into hepatic triacylglycerol by the intact animal. Homogenates of hepatocyte monolayers exposed to hydrazine in vitro also exhibited an increased capacity to form triacylglycerol from sn-[1,3-14C]glycerol 3-phosphate. Hydrazine-dependent increases in hepatic triacylglycerol production measured in vitro correlated well with an increase in microsomal phosphatidate phosphohydrolase (EC 3.1.3.4) activity. Therefore, the fatty liver associated with hydrazine exposure may be explained in part by a rise in the enzymatic capacity of hepatic triacylglycerol biosynthesis."}
{"id": "PMID:486521", "title": "Subcellular localization of three degeneration-associated phospholipids in cultured hamster fibroblasts (BHK21 cells).", "content": "Subcellular localization of bisphosphatidic acid, semilysobisphosphatidic acid and phosphatidyl-(N-acyl)ethanolamine was studied in normal and degenerating fibroblasts (BHK21 cells) by differential centrifugation. In the normal cells these lipids were highly enriched in the floating fraction consisting mainly of neutral lipid-rich lysosomes. They were also enriched in the mitochondrial fraction. In degenerating cells the high enrichment in the floating fraction was retained, but the other peak was displaced to the crude nuclear fraction. Subfractionation of the crude nuclear fraction indicated that these lipids were not enriched in the purified nuclei. Instead, their concentrations were relatively high in the other subfraction evidently enriched in the large secondary lysosomes characteristic for the degenerating cells. Neither in normal nor degenerating cells were these lipids enriched in the light mitochondrial fraction, where most of the smaller, and probably younger, lysosomes were found. On the basis of these results it is suggested that bisphosphatidic acid, semilysobisphosphatidic acid and phosphatidyl-(N-acyl)ethanolamine are lysosomal in origin. It appears possible that they are specifically associated with the organelles representing the later stages in the lysosomal lifespan.", "contents": "Subcellular localization of three degeneration-associated phospholipids in cultured hamster fibroblasts (BHK21 cells). Subcellular localization of bisphosphatidic acid, semilysobisphosphatidic acid and phosphatidyl-(N-acyl)ethanolamine was studied in normal and degenerating fibroblasts (BHK21 cells) by differential centrifugation. In the normal cells these lipids were highly enriched in the floating fraction consisting mainly of neutral lipid-rich lysosomes. They were also enriched in the mitochondrial fraction. In degenerating cells the high enrichment in the floating fraction was retained, but the other peak was displaced to the crude nuclear fraction. Subfractionation of the crude nuclear fraction indicated that these lipids were not enriched in the purified nuclei. Instead, their concentrations were relatively high in the other subfraction evidently enriched in the large secondary lysosomes characteristic for the degenerating cells. Neither in normal nor degenerating cells were these lipids enriched in the light mitochondrial fraction, where most of the smaller, and probably younger, lysosomes were found. On the basis of these results it is suggested that bisphosphatidic acid, semilysobisphosphatidic acid and phosphatidyl-(N-acyl)ethanolamine are lysosomal in origin. It appears possible that they are specifically associated with the organelles representing the later stages in the lysosomal lifespan."}
{"id": "PMID:486523", "title": "The neutral glyceroglucolipids of alveolar lavage from rabbit.", "content": "Three individual glycolipids have been isolated from the neutral lipid fraction of rabbit alveolar lavage. All three glycolipids contained glucose, glyceryl monoethers and fatty acids, and differed from each other primarily with respect to the number of glucose residues. The structures of these glycolipids were identified by mild alkaline methanolysis, oxidation with periodate and CrO3, and methylation studies, as: Glc(alpha 1 leads to 3)-1,(3)-O-alkyl-2-O-acylglycerol, Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 lead to 6)Glc(alpha 1 leads to 3)-1,(3)-O-alkyl-2-O-acyglycerol, and Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 3)-l,(3)-O-alkyl-2-O-acylglycerol.", "contents": "The neutral glyceroglucolipids of alveolar lavage from rabbit. Three individual glycolipids have been isolated from the neutral lipid fraction of rabbit alveolar lavage. All three glycolipids contained glucose, glyceryl monoethers and fatty acids, and differed from each other primarily with respect to the number of glucose residues. The structures of these glycolipids were identified by mild alkaline methanolysis, oxidation with periodate and CrO3, and methylation studies, as: Glc(alpha 1 leads to 3)-1,(3)-O-alkyl-2-O-acylglycerol, Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 lead to 6)Glc(alpha 1 leads to 3)-1,(3)-O-alkyl-2-O-acyglycerol, and Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 6)Glc(alpha 1 leads to 3)-l,(3)-O-alkyl-2-O-acylglycerol."}
{"id": "PMID:486524", "title": "The role of lysophosphatidylcholine and apolipoprotein A in the cholesterol-removing capacity of lipoprotein-deficient serum in tissue culture.", "content": "Lipoprotein-deficient serum (d greater than 1.21 or 1.25 g/ml fraction) is commonly used to deplete cellular cholesterol from cultured cells and presently we have studied some of the potential promoters of this process. Although serum albumin is the main protein component of the fraction, its cholesterol-removing capacity was quite limited, even in the presence of lysophosphatidylcholine, which is the major phospholipid of the d greater than 1.25 g/ml infranatant of serum. On the other hand, apolipoprotein A1 especially when complexed with lysophosphatidylcholine promoted considerable release of cellular cholesterol. The cholesterol-removing capacity of lysophosphatidylcholine alone was related to the fatty acid chain length and was low when the fatty acid chain length was below C-14. The release of cellular cholesterol is not related to shedding of surface glycoproteins and depends on the presence of suitable acceptors in the medium. Such acceptors were presently found in an ultrafiltrate of serum prepared by membrane filtration. It is proposed that in human serum there are low molecular weight protein-phospholipid complexes (less than 100,000), which can cross the capillary endothelial barrier, in preference to lipoproteins, and promote cholesterol removal from peripheral cells.", "contents": "The role of lysophosphatidylcholine and apolipoprotein A in the cholesterol-removing capacity of lipoprotein-deficient serum in tissue culture. Lipoprotein-deficient serum (d greater than 1.21 or 1.25 g/ml fraction) is commonly used to deplete cellular cholesterol from cultured cells and presently we have studied some of the potential promoters of this process. Although serum albumin is the main protein component of the fraction, its cholesterol-removing capacity was quite limited, even in the presence of lysophosphatidylcholine, which is the major phospholipid of the d greater than 1.25 g/ml infranatant of serum. On the other hand, apolipoprotein A1 especially when complexed with lysophosphatidylcholine promoted considerable release of cellular cholesterol. The cholesterol-removing capacity of lysophosphatidylcholine alone was related to the fatty acid chain length and was low when the fatty acid chain length was below C-14. The release of cellular cholesterol is not related to shedding of surface glycoproteins and depends on the presence of suitable acceptors in the medium. Such acceptors were presently found in an ultrafiltrate of serum prepared by membrane filtration. It is proposed that in human serum there are low molecular weight protein-phospholipid complexes (less than 100,000), which can cross the capillary endothelial barrier, in preference to lipoproteins, and promote cholesterol removal from peripheral cells."}
{"id": "PMID:486525", "title": "Growth-related lipid peroxidation in tumour microsomal membranes and mitochondria.", "content": "Microsomes and mitochondria isolated from Morris hepatomas 3924A (fast-growing) and 44 (slow-growing) and Ehrlich ascites tumour cells exhibit a NADPH-dependent peroxidation of endogenous lipids lower than that of the corresponding fractions from rat liver. Moreover, the O2- and ascorbate-dependent lipid peroxidations are decreased in microsomes from the two Morris hepatomas. The peroxidative activity appears to be inversely related to the growth rate of the tumours. It is suggested that the low susceptibility of tumour membranes to peroxidative agents may be a factor responsible for the high mitotic activity of this tissue.", "contents": "Growth-related lipid peroxidation in tumour microsomal membranes and mitochondria. Microsomes and mitochondria isolated from Morris hepatomas 3924A (fast-growing) and 44 (slow-growing) and Ehrlich ascites tumour cells exhibit a NADPH-dependent peroxidation of endogenous lipids lower than that of the corresponding fractions from rat liver. Moreover, the O2- and ascorbate-dependent lipid peroxidations are decreased in microsomes from the two Morris hepatomas. The peroxidative activity appears to be inversely related to the growth rate of the tumours. It is suggested that the low susceptibility of tumour membranes to peroxidative agents may be a factor responsible for the high mitotic activity of this tissue."}
{"id": "PMID:486526", "title": "Two gel states of cerebrosides. Calorimetric and Raman spectroscopic evidence.", "content": "The enthalpy of the gel-to-liquid crystalline phase transition for kerasin (15.8 kcal/mol) is found to be markedly higher than that for phrenosin and unfractionated bovine brain cerebrosides (about 7 kcal/mol). Evidence for a higher degree of order in the hydrocarbon chains and a different configuration in the polar region of kerasin is supplied by Raman spectroscopic parameters for these gel phases. The high transition enthalpy for kerasin is ascribed to a lesser accommodation of gauche conformers in the hydrocarbon chains just below the transition temperature. The thermodynamic behavior of these cerebroside fractions, including hysteresis in kerasin gels, is compared to that previously reported for sphingomyelins.", "contents": "Two gel states of cerebrosides. Calorimetric and Raman spectroscopic evidence. The enthalpy of the gel-to-liquid crystalline phase transition for kerasin (15.8 kcal/mol) is found to be markedly higher than that for phrenosin and unfractionated bovine brain cerebrosides (about 7 kcal/mol). Evidence for a higher degree of order in the hydrocarbon chains and a different configuration in the polar region of kerasin is supplied by Raman spectroscopic parameters for these gel phases. The high transition enthalpy for kerasin is ascribed to a lesser accommodation of gauche conformers in the hydrocarbon chains just below the transition temperature. The thermodynamic behavior of these cerebroside fractions, including hysteresis in kerasin gels, is compared to that previously reported for sphingomyelins."}
{"id": "PMID:486527", "title": "Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding properties from cartilage.", "content": "Partially degraded hyaluronate was coupled to AH-Sepharose 4B using carbodiimide. Approximately 1 mg of hyaluronate was incorporated per ml of wet gel. The derivatized gel was used to purify components of the hyaluronate-proteoglycan complex of cartilage. Two link-proteins were isolated from a crude cartilage extract by affinity binding to the gel and eluted with 4 M guanidinium chloride. By the same procedure one link-protein and the globular portion of the proteoglycan monomer were isolated from a trypsin-treated cartilage extract and were separated from each other by subsequent gel chromatography on Sepharose 6B and Sephacryl S-200. The affinity technique was also used for the preparation of these proteins labelled with dansyl groups.", "contents": "Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding properties from cartilage. Partially degraded hyaluronate was coupled to AH-Sepharose 4B using carbodiimide. Approximately 1 mg of hyaluronate was incorporated per ml of wet gel. The derivatized gel was used to purify components of the hyaluronate-proteoglycan complex of cartilage. Two link-proteins were isolated from a crude cartilage extract by affinity binding to the gel and eluted with 4 M guanidinium chloride. By the same procedure one link-protein and the globular portion of the proteoglycan monomer were isolated from a trypsin-treated cartilage extract and were separated from each other by subsequent gel chromatography on Sepharose 6B and Sephacryl S-200. The affinity technique was also used for the preparation of these proteins labelled with dansyl groups."}
{"id": "PMID:486528", "title": "The conformation of histone H5 bound to DNA. Maintenance of the globular structure after binding.", "content": "Trypsin digestion is used to investigate the conformation of histone H5 when bound to DNA. A central region of H5 comprising residues (22--100) is found to be resistant to digestion and it is concluded that this region is compacted whilst the remaining N- and C-terminal regions are more extended. Since this is the same result found previously for the free solution conformation of histone H5 it follows that a 3-domain structure is preserved on DNA binding. The binding of H5 and the central region (22--100) to DNA is also studied using proton magnetic resonance (270 MHz) and a precipitation approach. It is concluded that all 3 domains of H5 bind to DNA at low ionic strengths. The central domain (residues 22--100) is released at 0.3--0.4 M NaCl, but 0.7 M NaCl is required to release the N- and C-terminal regions. Comparison is made of H5 binding to DNA with that of the related histone H1.", "contents": "The conformation of histone H5 bound to DNA. Maintenance of the globular structure after binding. Trypsin digestion is used to investigate the conformation of histone H5 when bound to DNA. A central region of H5 comprising residues (22--100) is found to be resistant to digestion and it is concluded that this region is compacted whilst the remaining N- and C-terminal regions are more extended. Since this is the same result found previously for the free solution conformation of histone H5 it follows that a 3-domain structure is preserved on DNA binding. The binding of H5 and the central region (22--100) to DNA is also studied using proton magnetic resonance (270 MHz) and a precipitation approach. It is concluded that all 3 domains of H5 bind to DNA at low ionic strengths. The central domain (residues 22--100) is released at 0.3--0.4 M NaCl, but 0.7 M NaCl is required to release the N- and C-terminal regions. Comparison is made of H5 binding to DNA with that of the related histone H1."}
{"id": "PMID:486529", "title": "Structural studies on the carbohydrate units of armadillo submandibular glycoprotein.", "content": "The structure of carbohydrate units of the major glycoprotein fraction of armadillo submandibular gland was investigated. Alkaline borohydride reductive cleavage of the glycoprotein resulted in the release of O-glycosidically linked mono- and disaccharide units. The monosaccharide was identified as N-acetylgalactosaminitol, whereas disaccharide contained of N-acetylneuraminic acid and N-acetylgalactosaminitol. Treatment of the native and desialyzed glycoprotein with alpha-N-acetylgalactosaminidase resulted in the removal of 60% and 96% of N-acetylgalactosamine, respectively. No cleavage of this sugar was affected by the action of beta-N-acetylhexosaminidase. Both N-acetylgalactosamine and N-acetylneuraminic acid were susceptible to oxidation with periodate. Analyses of the partially methylated N-acetylgalactosamine derivatives, obtained from the permethylated native glycoprotein, showed the presence of 3,4,6-tri-O-methyl-N-methylacetamidogalactose and 3,4-di-O-methyl-N-methylacetamidogalactose in a ratio of 1 : 0.4. Only 3,4,6-tri-O-methyl-N-methylacetamidogalactose was found in the hydrolysates of permethylated desialyzed glycoprotein. These results together with our previous data on chemical composition of the glycoprotein suggest that about 30% of the oligosaccharide chains consist of NeuAc alpha 2 leads to 6GalNAc alpha 1 leads to O-Thr(Ser) and 70% of GalNAc alpha leads to O-Thr(Ser).", "contents": "Structural studies on the carbohydrate units of armadillo submandibular glycoprotein. The structure of carbohydrate units of the major glycoprotein fraction of armadillo submandibular gland was investigated. Alkaline borohydride reductive cleavage of the glycoprotein resulted in the release of O-glycosidically linked mono- and disaccharide units. The monosaccharide was identified as N-acetylgalactosaminitol, whereas disaccharide contained of N-acetylneuraminic acid and N-acetylgalactosaminitol. Treatment of the native and desialyzed glycoprotein with alpha-N-acetylgalactosaminidase resulted in the removal of 60% and 96% of N-acetylgalactosamine, respectively. No cleavage of this sugar was affected by the action of beta-N-acetylhexosaminidase. Both N-acetylgalactosamine and N-acetylneuraminic acid were susceptible to oxidation with periodate. Analyses of the partially methylated N-acetylgalactosamine derivatives, obtained from the permethylated native glycoprotein, showed the presence of 3,4,6-tri-O-methyl-N-methylacetamidogalactose and 3,4-di-O-methyl-N-methylacetamidogalactose in a ratio of 1 : 0.4. Only 3,4,6-tri-O-methyl-N-methylacetamidogalactose was found in the hydrolysates of permethylated desialyzed glycoprotein. These results together with our previous data on chemical composition of the glycoprotein suggest that about 30% of the oligosaccharide chains consist of NeuAc alpha 2 leads to 6GalNAc alpha 1 leads to O-Thr(Ser) and 70% of GalNAc alpha leads to O-Thr(Ser)."}
{"id": "PMID:486530", "title": "Carbon-13 NMR investigations on ribonuclease A.", "content": "The proposed interaction between the amino acid residues Asp 14 and His 48 of ribonuclease A has been confirmed by 13C-NMR spectroscopy. The titration behaviour of the resonance of the side-chain carboxyl group of Asp 14 suggests a pKa of 6.5--7.0 for His 48. An equilibrium between different conformation process of His 48. Upon this deprotonation a hydrogen bond between the side-chains of Asp 14 or His 48 and Tyr 25 seems to be formed as is suggested by the behaviour of a tyrosine C zeta resonance assigned to Tyr 25. One phenylalanine resonance broadens and moves upfield on the addition of the inhibitor Cyd-2'-P, being therefore assigned to Phe 120. The behaviour of this resonance suggests that the upfield shift results from the anisotropy of the cytidine ring. Three signals are assigned to the three Phe residues.", "contents": "Carbon-13 NMR investigations on ribonuclease A. The proposed interaction between the amino acid residues Asp 14 and His 48 of ribonuclease A has been confirmed by 13C-NMR spectroscopy. The titration behaviour of the resonance of the side-chain carboxyl group of Asp 14 suggests a pKa of 6.5--7.0 for His 48. An equilibrium between different conformation process of His 48. Upon this deprotonation a hydrogen bond between the side-chains of Asp 14 or His 48 and Tyr 25 seems to be formed as is suggested by the behaviour of a tyrosine C zeta resonance assigned to Tyr 25. One phenylalanine resonance broadens and moves upfield on the addition of the inhibitor Cyd-2'-P, being therefore assigned to Phe 120. The behaviour of this resonance suggests that the upfield shift results from the anisotropy of the cytidine ring. Three signals are assigned to the three Phe residues."}
{"id": "PMID:486531", "title": "Ribosomes of Physarum polycephalum. Subunits and protein composition.", "content": "Ribosomes from Physarum polycephalum were purified. Optimal conditions for preparation and stability of subunits were determined. KCl concentration above 200 mM induced protein dissociation from the subunits. It was observed that dissociated ribosomes were more stable in a low ionic strength buffer than in 200 mM KCl, where the 40 S was preferentially degraded by ribonucleases. Ribosomal proteins were analyzed by two-dimensional gel electrophoresis. The first dimension was carried out at pH 8.6 while the second was run at pH 4.6. The monosome contained sixty seven proteins, of which six were acidic. Two proteins were lost after subunit dissociation. Twenty six basic and two acidic proteins were observed in the 40 S subunit while the largest subunit gave thirty nine spots on the basic part of the gel and three additional spots on the acidic side. Five proteins were shared by 40 S and 60 S.", "contents": "Ribosomes of Physarum polycephalum. Subunits and protein composition. Ribosomes from Physarum polycephalum were purified. Optimal conditions for preparation and stability of subunits were determined. KCl concentration above 200 mM induced protein dissociation from the subunits. It was observed that dissociated ribosomes were more stable in a low ionic strength buffer than in 200 mM KCl, where the 40 S was preferentially degraded by ribonucleases. Ribosomal proteins were analyzed by two-dimensional gel electrophoresis. The first dimension was carried out at pH 8.6 while the second was run at pH 4.6. The monosome contained sixty seven proteins, of which six were acidic. Two proteins were lost after subunit dissociation. Twenty six basic and two acidic proteins were observed in the 40 S subunit while the largest subunit gave thirty nine spots on the basic part of the gel and three additional spots on the acidic side. Five proteins were shared by 40 S and 60 S."}
{"id": "PMID:486532", "title": "Seeding role of spectrin in polymerization of skeletal muscle actin.", "content": "The effect of spectrin on the polymerization of muscle actin has been investigated by hydrodynamic methods and electron microscopy. Spectrin markedly accelerated polymerization of actin. The effect was more easily observed in lower concentrations of KCl (e.g. 24 mM) where spontaneous polymerization was negligibly small. Similarly large acceleration was observed for polymerization in MgCl2 or CaCl2. The rate of polymerization of actin was proportionally increased with the concentration of spectrin added to a fixed concentration of action. The stationary level of specific viscosity also increased with the spectrin concentration, but at larger concentrations it became smaller. The flow birefringence and electron microscope measurements indicated that actin polymers formed under the influence of spectrin were shorter than those of control F-actin filaments. The structural viscosity and electron microscope observations suggested that the interaction between F-actin fibers was not increased by spectrin. These data strongly suggest a seeding role of spectrin in the polymerization of actin. Spectrin accelerates formation of the nuclei for polymerization. The more the nuclei are formed, the larger the number of the grown polymers are and this leads to rapid formation of shorter polymers since the amount of actin is limited. The acceleration activity was found only in freshly prepared spectrin from fresh ghosts taken from freshly drawn blood.", "contents": "Seeding role of spectrin in polymerization of skeletal muscle actin. The effect of spectrin on the polymerization of muscle actin has been investigated by hydrodynamic methods and electron microscopy. Spectrin markedly accelerated polymerization of actin. The effect was more easily observed in lower concentrations of KCl (e.g. 24 mM) where spontaneous polymerization was negligibly small. Similarly large acceleration was observed for polymerization in MgCl2 or CaCl2. The rate of polymerization of actin was proportionally increased with the concentration of spectrin added to a fixed concentration of action. The stationary level of specific viscosity also increased with the spectrin concentration, but at larger concentrations it became smaller. The flow birefringence and electron microscope measurements indicated that actin polymers formed under the influence of spectrin were shorter than those of control F-actin filaments. The structural viscosity and electron microscope observations suggested that the interaction between F-actin fibers was not increased by spectrin. These data strongly suggest a seeding role of spectrin in the polymerization of actin. Spectrin accelerates formation of the nuclei for polymerization. The more the nuclei are formed, the larger the number of the grown polymers are and this leads to rapid formation of shorter polymers since the amount of actin is limited. The acceleration activity was found only in freshly prepared spectrin from fresh ghosts taken from freshly drawn blood."}
{"id": "PMID:486533", "title": "Epidermal proteins of cultured human and bovine keratinocytes.", "content": "Bovine and human epidermal cells were cultured on mitomycin C treated fibroblasts. The cells were carried through four passages and found to synthesize fibrous proteins and insoluble cell envelopes. Acid buffer soluble fibrous protein, prekeratin, and urea soluble fibrous protein were both identified and the latter was the major component in older cultures. Some of the prekeratin polypeptides of intact tissue were not found in cultured cells, but the ones that  were present corresponded to those of whole tissue. X-ray diffraction, amino acid analysis and immunological techniques were used to establish that the polypeptides were keratins. The insoluble cell envelopes had a higher proline and 1/2 cystine content than the fibrous protein, similar to what is found in whole epidermis. Histidase, a characteristic enzyme marker of whole epidermis, was not observed in cultured cells. These studies indicate that differentiation occurs in cultured cells but it may not be as complete as in intact tissue.", "contents": "Epidermal proteins of cultured human and bovine keratinocytes. Bovine and human epidermal cells were cultured on mitomycin C treated fibroblasts. The cells were carried through four passages and found to synthesize fibrous proteins and insoluble cell envelopes. Acid buffer soluble fibrous protein, prekeratin, and urea soluble fibrous protein were both identified and the latter was the major component in older cultures. Some of the prekeratin polypeptides of intact tissue were not found in cultured cells, but the ones that  were present corresponded to those of whole tissue. X-ray diffraction, amino acid analysis and immunological techniques were used to establish that the polypeptides were keratins. The insoluble cell envelopes had a higher proline and 1/2 cystine content than the fibrous protein, similar to what is found in whole epidermis. Histidase, a characteristic enzyme marker of whole epidermis, was not observed in cultured cells. These studies indicate that differentiation occurs in cultured cells but it may not be as complete as in intact tissue."}
{"id": "PMID:486534", "title": "[Multiple molecular forms of human plasma butyrylcholinesterase. I. Apparent molecular parameters and broad pattern of the quaternary structure (author's transl)].", "content": "Apparent molecular parameters (molecular weights, sedimentation constants, partial specific volumes, free electrophoretic mobilities and isoelectric points) of the four molecular forms C-1, C-2, C-3 and C-4 of human plasma butyrylcholinesterase (EC 3.1.1.8) have been demonstrated by polyacrylamide gel electrophoresis methods and centrifugation in sucrose gradient. The C-1 component is the monomeric form of the enzyme )Mr = 84 800 +/- 5800). All the forms are partially interconvertible and C-1, C-3, C-4 are size isomers corresponding to the monomer, dimer and tetramer of the enzyme. An estimation of the general shape of these forms attempted from electrophoretic and hydrodynamic parameters suggests that they are prolate ellipsoids. The C-4 component in which the axial ratio is at least equal to 8 appears to be arranged as a dimer of dimers (C-3)2 in which the two units are associated in a quasi-linear fashion. The C-2 component is composed of C-1 associated with an inactive smaller subunit, which is responsible for its specific electrical properties (mobility and isoelectric point).", "contents": "[Multiple molecular forms of human plasma butyrylcholinesterase. I. Apparent molecular parameters and broad pattern of the quaternary structure (author's transl)]. Apparent molecular parameters (molecular weights, sedimentation constants, partial specific volumes, free electrophoretic mobilities and isoelectric points) of the four molecular forms C-1, C-2, C-3 and C-4 of human plasma butyrylcholinesterase (EC 3.1.1.8) have been demonstrated by polyacrylamide gel electrophoresis methods and centrifugation in sucrose gradient. The C-1 component is the monomeric form of the enzyme )Mr = 84 800 +/- 5800). All the forms are partially interconvertible and C-1, C-3, C-4 are size isomers corresponding to the monomer, dimer and tetramer of the enzyme. An estimation of the general shape of these forms attempted from electrophoretic and hydrodynamic parameters suggests that they are prolate ellipsoids. The C-4 component in which the axial ratio is at least equal to 8 appears to be arranged as a dimer of dimers (C-3)2 in which the two units are associated in a quasi-linear fashion. The C-2 component is composed of C-1 associated with an inactive smaller subunit, which is responsible for its specific electrical properties (mobility and isoelectric point)."}
{"id": "PMID:486535", "title": "A kinetic study of the folding of nuclease B, a possible precursor of staphylococcal nuclease A.", "content": "Nuclease B, which contains an additional flexible amino acid sequence of 19 amino acid residues bound to the NH2-terminus of nuclease A, an extracellular nuclease of Staphylococcus aureus, has been investigated in order to determine the influence of the extra residues on the refolding of the nuclease A portion from the acid denaturated state by monitoring the change in tryptophan fluorescence using a stopped-flow technique. It was found that the kinetic parameters of this refolding is similar within experimental error for nuclease A and nuclease B for the entire course (up to 40 s) studied. Therefore, the extra residues do not appear to have any detectable effect on the dynamic events involved in the refolding process. Thus, the folding of the nuclease A portion of nuclease B appears to be thermodynamically and kinetically independent of the 19 residues at the amino-terminus.", "contents": "A kinetic study of the folding of nuclease B, a possible precursor of staphylococcal nuclease A. Nuclease B, which contains an additional flexible amino acid sequence of 19 amino acid residues bound to the NH2-terminus of nuclease A, an extracellular nuclease of Staphylococcus aureus, has been investigated in order to determine the influence of the extra residues on the refolding of the nuclease A portion from the acid denaturated state by monitoring the change in tryptophan fluorescence using a stopped-flow technique. It was found that the kinetic parameters of this refolding is similar within experimental error for nuclease A and nuclease B for the entire course (up to 40 s) studied. Therefore, the extra residues do not appear to have any detectable effect on the dynamic events involved in the refolding process. Thus, the folding of the nuclease A portion of nuclease B appears to be thermodynamically and kinetically independent of the 19 residues at the amino-terminus."}
{"id": "PMID:486536", "title": "A new abnormal human hemoglobin: Hb Prato (alpha 2 31 (B12) Arg leads to Ser beta 2).", "content": "An abnormal human hemoglobin was found in a hemolysate from a 5-year-old healthy child living in Prato (Tuscany, Italy). Strutctural studies demonstrated a previously unreported amino acid substitution, alpha 31 (B12) Arg leads to Ser (this is an alpha 1 beta 1 contact). The new variant has been named Hb Prato. It was unstable in isopropanol and heat-denaturation tests, but has normal functional properties, with respect to whole blood studies. Family studies indicated that the variant had been inherited from the mother, a 39-year-old woman of Sicilian extraction. Hb Prato occurs at 20 and 28% in hemolysates from the boy and woman, respectively.", "contents": "A new abnormal human hemoglobin: Hb Prato (alpha 2 31 (B12) Arg leads to Ser beta 2). An abnormal human hemoglobin was found in a hemolysate from a 5-year-old healthy child living in Prato (Tuscany, Italy). Strutctural studies demonstrated a previously unreported amino acid substitution, alpha 31 (B12) Arg leads to Ser (this is an alpha 1 beta 1 contact). The new variant has been named Hb Prato. It was unstable in isopropanol and heat-denaturation tests, but has normal functional properties, with respect to whole blood studies. Family studies indicated that the variant had been inherited from the mother, a 39-year-old woman of Sicilian extraction. Hb Prato occurs at 20 and 28% in hemolysates from the boy and woman, respectively."}
{"id": "PMID:486537", "title": "Fluorescence quenching of tryptophan and related compounds by hydrogen peroxide.", "content": "The fluorescence of tryptophan and some related derivatives was found to be quenched by hydrogen peroxide. The quenching mechanism was shown to be essentially dynamic in nature, without any ground-state complex formation, and it is interpreted as resulting through an electron transfer from the excited indole ring to hydrogen peroxide.", "contents": "Fluorescence quenching of tryptophan and related compounds by hydrogen peroxide. The fluorescence of tryptophan and some related derivatives was found to be quenched by hydrogen peroxide. The quenching mechanism was shown to be essentially dynamic in nature, without any ground-state complex formation, and it is interpreted as resulting through an electron transfer from the excited indole ring to hydrogen peroxide."}
{"id": "PMID:486538", "title": "Induction of alkaline phosphatase in cultured human fibroblasts. Comparison of normal cells and those from patients with cystic fibrosis.", "content": "The membrane glycoprotein enzyme, alkaline phosphatase was induced in cultured human fibroblasts by dibutyryl cyclic AMP, sodium butyrate, the serum glycoprotein fetuin, the Tamm-Horsfall urinary glycoprotein, and by a number of inhibitors of DNA synthesis. The uninduced basal enzyme activity increased at later stages of growth when the cells became confluent. Induction by dibutyryl cyclic AMP or fetuin was most effective when the agents were added after the cells had reached stationary phase and was maximal after at least two days of exposure. The levels of induction resulting from the addition of pairs of the agents, dibutyryl cyclic AMP, n-butyrate and fetuin were additive indicating that these have different modes of action. The inhibitors of DNA synthesis, cytosine arabinoside, hydroxyurea, and methothrexate were less effective inducers. Bromodeoxyuridine which also has non-DNA mediated effects induced to the same extent as dibutyryl cyclic AMP. Similar experiments with sex- and age-matched cell strains derived from patients with cystic fibrosis failed to detect differences in the levels of induction from those observed in normal cells. In addition, the combined inductive effects of Tamm-Horsfall glycoprotein, isoproterenol and theophylline, were similar with normal and cystic fibrosis cells.", "contents": "Induction of alkaline phosphatase in cultured human fibroblasts. Comparison of normal cells and those from patients with cystic fibrosis. The membrane glycoprotein enzyme, alkaline phosphatase was induced in cultured human fibroblasts by dibutyryl cyclic AMP, sodium butyrate, the serum glycoprotein fetuin, the Tamm-Horsfall urinary glycoprotein, and by a number of inhibitors of DNA synthesis. The uninduced basal enzyme activity increased at later stages of growth when the cells became confluent. Induction by dibutyryl cyclic AMP or fetuin was most effective when the agents were added after the cells had reached stationary phase and was maximal after at least two days of exposure. The levels of induction resulting from the addition of pairs of the agents, dibutyryl cyclic AMP, n-butyrate and fetuin were additive indicating that these have different modes of action. The inhibitors of DNA synthesis, cytosine arabinoside, hydroxyurea, and methothrexate were less effective inducers. Bromodeoxyuridine which also has non-DNA mediated effects induced to the same extent as dibutyryl cyclic AMP. Similar experiments with sex- and age-matched cell strains derived from patients with cystic fibrosis failed to detect differences in the levels of induction from those observed in normal cells. In addition, the combined inductive effects of Tamm-Horsfall glycoprotein, isoproterenol and theophylline, were similar with normal and cystic fibrosis cells."}
{"id": "PMID:486539", "title": "Interactions between heparin and factor Xa. Inhibition of prothrombin activation.", "content": "The effects of heparin on prothrombin activation have been examined. Heparin was found to inhibit the rate of prothrombin activation by Factor Xa, calcium and phospholipid. In the absence of phospholipid, heparin had no effect on the rate of prothrombin activation. In contrast, heparin was found to increase the rate of activation of prethrombin-1 and prethrombin-2. Initial velocity studies indicated that heparin blocks lipid stimulation of prothrombin activation. In accord with this, binding studies demonstrated that heparin could displace Factor Xa, and in separate experiments, prothrombin, from phospholipid vesicles.", "contents": "Interactions between heparin and factor Xa. Inhibition of prothrombin activation. The effects of heparin on prothrombin activation have been examined. Heparin was found to inhibit the rate of prothrombin activation by Factor Xa, calcium and phospholipid. In the absence of phospholipid, heparin had no effect on the rate of prothrombin activation. In contrast, heparin was found to increase the rate of activation of prethrombin-1 and prethrombin-2. Initial velocity studies indicated that heparin blocks lipid stimulation of prothrombin activation. In accord with this, binding studies demonstrated that heparin could displace Factor Xa, and in separate experiments, prothrombin, from phospholipid vesicles."}
{"id": "PMID:486540", "title": "Inhibition of factor VIII-associated platelet aggregation by heparin and dextran sulfate, and its mechanism.", "content": "Both ristocetin-induced aggregation in the presence of human factor VIII and bovine factor VIII-induced aggregation of washed normal human platelets were inhibited or reversed by the addition of heparin or dextran sulfate. These actions of dextran sulfate were stronger than those of heparin, and dependent on the sulfur content of dextran sulfate. In order to study the mechanism of actions of dextran sulfate and heparin, the affinity chromatographic experiment of factor VIII in human and bovine plasma, respectively, was carried out by using a dextran sulfate- and a heparin-Agarose column. Both human and bovine factor VIII have a strong affinity for dextran sulfate with high sulfur content and a weak affinity for heparin, but no affinity for dextran sulfate with low sulfur content. From these results, it is suggested that dextran sulfate or heparin binds directly the human and bovine factor VIII, which is an essential factor for the maintenance of the weak interplatelet bonds, and either inhibits or reverses the platelet aggregation.", "contents": "Inhibition of factor VIII-associated platelet aggregation by heparin and dextran sulfate, and its mechanism. Both ristocetin-induced aggregation in the presence of human factor VIII and bovine factor VIII-induced aggregation of washed normal human platelets were inhibited or reversed by the addition of heparin or dextran sulfate. These actions of dextran sulfate were stronger than those of heparin, and dependent on the sulfur content of dextran sulfate. In order to study the mechanism of actions of dextran sulfate and heparin, the affinity chromatographic experiment of factor VIII in human and bovine plasma, respectively, was carried out by using a dextran sulfate- and a heparin-Agarose column. Both human and bovine factor VIII have a strong affinity for dextran sulfate with high sulfur content and a weak affinity for heparin, but no affinity for dextran sulfate with low sulfur content. From these results, it is suggested that dextran sulfate or heparin binds directly the human and bovine factor VIII, which is an essential factor for the maintenance of the weak interplatelet bonds, and either inhibits or reverses the platelet aggregation."}
{"id": "PMID:486541", "title": "Studies of ethylene-forming system in rat liver extract.", "content": "Evidence of enzymatic formation of ethylene from methionine by rat liver extracts is presented. The ethylene production is closely associated with growth of the animal. The conversion of L-methionine to ehtylene is oxygen dependent. Substrate analogue studies show that the ethylene-forming system is structurally specific and requires in the center of the molecule alpha-CH2-CH2- with one end attached to an unencumbered sulfur atom from a thioether moiety and the other end attached to a carboxyl group. Sylfhydryl agents are found to be very effective inhibitors of the ethylene-forming system. The finding of alpha-keto-4-methylthiobutyric acid to be a more efficient precursor of ethylene production suggests the possibility that alpha-keto-4-methylthiobutyric acid may be an intermediate in the biosynthesis of ethylene from methionine in mammalian tissues.", "contents": "Studies of ethylene-forming system in rat liver extract. Evidence of enzymatic formation of ethylene from methionine by rat liver extracts is presented. The ethylene production is closely associated with growth of the animal. The conversion of L-methionine to ehtylene is oxygen dependent. Substrate analogue studies show that the ethylene-forming system is structurally specific and requires in the center of the molecule alpha-CH2-CH2- with one end attached to an unencumbered sulfur atom from a thioether moiety and the other end attached to a carboxyl group. Sylfhydryl agents are found to be very effective inhibitors of the ethylene-forming system. The finding of alpha-keto-4-methylthiobutyric acid to be a more efficient precursor of ethylene production suggests the possibility that alpha-keto-4-methylthiobutyric acid may be an intermediate in the biosynthesis of ethylene from methionine in mammalian tissues."}
{"id": "PMID:486542", "title": "Differential fixation of poly(L-arginine) and poly(L-lysine) by tannic acid and its application to the fixation of collagen in electron microscopy.", "content": "A differential fixation of poly(L-arginine) and poly(L-lysine) has been demonstrated by means of cellulose acetate electrophoresis and colorimetric titration. Electrophoresis showed that at pH 3.0 and concentrations between 0.025% and 2% the reagent interacts with poly(L-arginine) but not with poly(L-lysine). At pH 7.5, however, poly(L-lysine) also reacts, although at a higher concentration of tannic acid than was required to fix poly(L-arginine) at this pH. Colorimetric titration revealed that for poly(L-arginine) the reaction with tannic acid commences at pH 3.0 and is complete at pH 4.1 whereas for poly(L-lysine) the reaction commences at pH 3.5 and is complete at pH 4.9. It is suggested that the reaction is predominantly electrostatic. The results are discussed in relation to the use of tannic acid as a protein fixative in electron microscopy.", "contents": "Differential fixation of poly(L-arginine) and poly(L-lysine) by tannic acid and its application to the fixation of collagen in electron microscopy. A differential fixation of poly(L-arginine) and poly(L-lysine) has been demonstrated by means of cellulose acetate electrophoresis and colorimetric titration. Electrophoresis showed that at pH 3.0 and concentrations between 0.025% and 2% the reagent interacts with poly(L-arginine) but not with poly(L-lysine). At pH 7.5, however, poly(L-lysine) also reacts, although at a higher concentration of tannic acid than was required to fix poly(L-arginine) at this pH. Colorimetric titration revealed that for poly(L-arginine) the reaction with tannic acid commences at pH 3.0 and is complete at pH 4.1 whereas for poly(L-lysine) the reaction commences at pH 3.5 and is complete at pH 4.9. It is suggested that the reaction is predominantly electrostatic. The results are discussed in relation to the use of tannic acid as a protein fixative in electron microscopy."}
{"id": "PMID:486543", "title": "A method for studying the depolarization-induced calcium ion release from fragmented sarcoplasmic reticulum.", "content": "An optical method of studying the 'depolarizatoin'-induced Ca2+ release from isolated sarcoplasmic reticulum was presented. The method, which involves the use of metallochromic indicators, has the advantage of being able to perform the rapid kinetic measurement of the release. It was suggested that the velocity of the 'depolarization'-induced Ca2+ release was rapid enough to account for the velocity of muscle contraction if the phenomenon is involved in the contraction mechanism. The change of membrane potential was also measured optically using a potential-sensitive dye. The possibility that this type of release is caused by osmotic effects is discussed.", "contents": "A method for studying the depolarization-induced calcium ion release from fragmented sarcoplasmic reticulum. An optical method of studying the 'depolarizatoin'-induced Ca2+ release from isolated sarcoplasmic reticulum was presented. The method, which involves the use of metallochromic indicators, has the advantage of being able to perform the rapid kinetic measurement of the release. It was suggested that the velocity of the 'depolarization'-induced Ca2+ release was rapid enough to account for the velocity of muscle contraction if the phenomenon is involved in the contraction mechanism. The change of membrane potential was also measured optically using a potential-sensitive dye. The possibility that this type of release is caused by osmotic effects is discussed."}
{"id": "PMID:486544", "title": "Multiple effects of guanine nucleotides on human platelet adenylated cyclase.", "content": "We report that the adenylate cyclase system in human platelets is subject to multiple regulation by guanine nucleotides. Previously it has been reported that GTP is either required for or has little effect on the response of the enzyme to prostaglandin E1. We have found that when platelet lysates were prepared in the presence of 5 mM EDTA, GTP lowered the basal and prostaglandin E1-stimulated adenylate cyclase activity, but at a higher concentration of Mn2+, it caused an increase in enzyme activity exceeding that occurring in the presence of prostaglandin E1. In the presence of Mn2+, dGTP mimics the effect of GTP and is 50% as effective as GTP. Our data suggest that the inhibitory effect of GTP on prostaglandin E1-stimulated adenylate cyclase is mainly due to its direct effect on the enzyme itself, whereas the stimulatory effect of GTP on prostaglandin E1-stimulated adenylate cyclase is due to enhancement of the coupling between the prostaglandin E1 receptor and adenylate cyclase. These studies also indicate that the method of preparation of platelet lysates can profoundly alter the nature of guanine nucleotide regulation of adenylate cyclase.", "contents": "Multiple effects of guanine nucleotides on human platelet adenylated cyclase. We report that the adenylate cyclase system in human platelets is subject to multiple regulation by guanine nucleotides. Previously it has been reported that GTP is either required for or has little effect on the response of the enzyme to prostaglandin E1. We have found that when platelet lysates were prepared in the presence of 5 mM EDTA, GTP lowered the basal and prostaglandin E1-stimulated adenylate cyclase activity, but at a higher concentration of Mn2+, it caused an increase in enzyme activity exceeding that occurring in the presence of prostaglandin E1. In the presence of Mn2+, dGTP mimics the effect of GTP and is 50% as effective as GTP. Our data suggest that the inhibitory effect of GTP on prostaglandin E1-stimulated adenylate cyclase is mainly due to its direct effect on the enzyme itself, whereas the stimulatory effect of GTP on prostaglandin E1-stimulated adenylate cyclase is due to enhancement of the coupling between the prostaglandin E1 receptor and adenylate cyclase. These studies also indicate that the method of preparation of platelet lysates can profoundly alter the nature of guanine nucleotide regulation of adenylate cyclase."}
{"id": "PMID:486545", "title": "Acetylcholine receptor. Binding properties and ion permeability response after covalent attachment of the local anaesthetic quinacrine.", "content": "Membrane vesicles rich in nicotinic acetylcholine receptor prepared from Torpedo californica electric tissue have been irreversibly modified with quinacrine mustard, an alkylating derivative of the local anaesthetic quinacrine. The reaction blocked the ion channel regulated by the acetylcholine receptor. Acetylcholine still bound to the modified membrane vesicles with KD approx. 10(-8). The number of binding sites was reduced by up to 50%. Stopped-flow experiments showed that in contrast to what had been found with the reversibly binding quinacrine no fluorescence changes caused by energy transfer from the irradiated protein to the fluorescent local anaesthetic occurred after addition of agonist. This indicates that the conformational changes associated with the activation of the ion channel are blocked by the covalent reaction with quinacrine mustard. Analysis of the membrane vesicles by SDS-polyacrylamide gel electrophoresis showed that all polypeptide chains assumed to be part of the receptor complex had reacted with the mustard. Even small components, probably lipids, migrating with the dye front, showed fluorescence.", "contents": "Acetylcholine receptor. Binding properties and ion permeability response after covalent attachment of the local anaesthetic quinacrine. Membrane vesicles rich in nicotinic acetylcholine receptor prepared from Torpedo californica electric tissue have been irreversibly modified with quinacrine mustard, an alkylating derivative of the local anaesthetic quinacrine. The reaction blocked the ion channel regulated by the acetylcholine receptor. Acetylcholine still bound to the modified membrane vesicles with KD approx. 10(-8). The number of binding sites was reduced by up to 50%. Stopped-flow experiments showed that in contrast to what had been found with the reversibly binding quinacrine no fluorescence changes caused by energy transfer from the irradiated protein to the fluorescent local anaesthetic occurred after addition of agonist. This indicates that the conformational changes associated with the activation of the ion channel are blocked by the covalent reaction with quinacrine mustard. Analysis of the membrane vesicles by SDS-polyacrylamide gel electrophoresis showed that all polypeptide chains assumed to be part of the receptor complex had reacted with the mustard. Even small components, probably lipids, migrating with the dye front, showed fluorescence."}
{"id": "PMID:486546", "title": "Effect of L-leucine on p-aminohippurate accumulation by mammalian renal cortical slices.", "content": "The effect of several amino acids (L-glutamate, L-phenylalanine, L-leucine, glycine, L-tryptophan, L-histidine, L-valine) on p-aminohippurate accumulation was evaluated in rat, mouse and rabbit kidneys. Only leucine was found to enhance p-aminohippurate accumulation in rat and mouse renal cortical slices but not in rabbit slices. Leucine had no effect on tetraethylammonium accumulation. In rat renal cortical slices, leucine increased the uptake and decreased the runout of p-aminohippurate, each effect contributing to the increase in p-aminohippurate accumulation. The apparent Km of p-aminohippurate uptake was decreased by leucine with no change in the apparent V. Aminooxyacetate (an inhibitor of transamination of leucine) partially depressed the stimulating effect of leucine on p-aminohippurate accumulation, whereas alpha-ketoisocaproic acid (a metabolite of leucine formed by transamination) enhanced p-aminohippurate accumulation, suggesting that the metabolism of leucine in kidney slices may be necessary for the stimulating effect on p-aminohippurate transport.", "contents": "Effect of L-leucine on p-aminohippurate accumulation by mammalian renal cortical slices. The effect of several amino acids (L-glutamate, L-phenylalanine, L-leucine, glycine, L-tryptophan, L-histidine, L-valine) on p-aminohippurate accumulation was evaluated in rat, mouse and rabbit kidneys. Only leucine was found to enhance p-aminohippurate accumulation in rat and mouse renal cortical slices but not in rabbit slices. Leucine had no effect on tetraethylammonium accumulation. In rat renal cortical slices, leucine increased the uptake and decreased the runout of p-aminohippurate, each effect contributing to the increase in p-aminohippurate accumulation. The apparent Km of p-aminohippurate uptake was decreased by leucine with no change in the apparent V. Aminooxyacetate (an inhibitor of transamination of leucine) partially depressed the stimulating effect of leucine on p-aminohippurate accumulation, whereas alpha-ketoisocaproic acid (a metabolite of leucine formed by transamination) enhanced p-aminohippurate accumulation, suggesting that the metabolism of leucine in kidney slices may be necessary for the stimulating effect on p-aminohippurate transport."}
{"id": "PMID:486547", "title": "Replenishment and nuclear retention of oestradiol-17 beta receptors in rat uteri during postnatal development.", "content": "1. Uteri of 6--10-day-old rats do not show a late growth response to oestrogen (increase in rate of DNA synthesis and cell division) exhibited by fully competent (20 days or older) uteri. We posed the question whether the lack of the late growth response is due to an inability to replenish the cytoplasmic pool of oestrogen receptors or to curtailed retention of oestrogen binding in the nucleus. Uterine nuclear and cytoplasmic receptors were measured by a [3H]oestradiol-17 beta exchange assay, at 1, 3, 6, 14 and 24 h after oestrogen injection. 2. The replenishment of cytoplasmic oestrogen receptors showed a similar pattern in the uteri of 6 and 10-day-old (partially responsive) and in 20-day-old (fully responsive) rats. 3. Oestrogen was retained longer in uterine nuclei obtained from 5 and 10-day-old rats than in uterine nuclei of 20 and 25-day-old rats. 4. Oestrogen receptors resistant to 0.4 M KCl extraction (residual receptors) were found in uterine nuclei of 6 and 25-day-old rats after oestrogen injection at all the times tested. The concentration of these residual receptors during the late period (6--24 h after injection) was not significantly different in uterine nuclei of 6-day-old and 25-day-old rats. 5. We conclude that neither lack of oestrogen receptor replenishment nor curtailed retention of oestrogen binding in the nucleus is the factor which limits the complete responsiveness to oestrogen in uteri of rats during postnatal development.", "contents": "Replenishment and nuclear retention of oestradiol-17 beta receptors in rat uteri during postnatal development. 1. Uteri of 6--10-day-old rats do not show a late growth response to oestrogen (increase in rate of DNA synthesis and cell division) exhibited by fully competent (20 days or older) uteri. We posed the question whether the lack of the late growth response is due to an inability to replenish the cytoplasmic pool of oestrogen receptors or to curtailed retention of oestrogen binding in the nucleus. Uterine nuclear and cytoplasmic receptors were measured by a [3H]oestradiol-17 beta exchange assay, at 1, 3, 6, 14 and 24 h after oestrogen injection. 2. The replenishment of cytoplasmic oestrogen receptors showed a similar pattern in the uteri of 6 and 10-day-old (partially responsive) and in 20-day-old (fully responsive) rats. 3. Oestrogen was retained longer in uterine nuclei obtained from 5 and 10-day-old rats than in uterine nuclei of 20 and 25-day-old rats. 4. Oestrogen receptors resistant to 0.4 M KCl extraction (residual receptors) were found in uterine nuclei of 6 and 25-day-old rats after oestrogen injection at all the times tested. The concentration of these residual receptors during the late period (6--24 h after injection) was not significantly different in uterine nuclei of 6-day-old and 25-day-old rats. 5. We conclude that neither lack of oestrogen receptor replenishment nor curtailed retention of oestrogen binding in the nucleus is the factor which limits the complete responsiveness to oestrogen in uteri of rats during postnatal development."}
{"id": "PMID:486548", "title": "Composition of the aqueous phase of chromaffin granules.", "content": "Nuclear magnetic resonance spectroscopy has been used to determine the composition of the aqueous phase of bovine chromaffin granules. Relative concentrations of catecholamines (epinephrine plus norepinephrine), ATP and chromogranins have been measured from integrated intensities in the proton spectra using computer simulation techniques. Most or all of the catecholamines (97 +/- 8%) are present in the aqueous phase and contribute to the high resolution spectrum. The catecholamine:ATP molar ratio (4.41 +/- 0.45) determined by NMR is close to the value (4.45) derived from biochemical assay indicating that most or all of the ATP is present with catecholamine in the aqueous phase. Catecholamine:protein ratios show that approximately 45% of the soluble protein freed by lysis is not NMR visible. Intensity from this fraction does not appear under highly denaturing conditions (8 M urea) but reappears after hydrolysis. This behavior is similar to that of recently isolated soluble lipoprotein complexes. Variations in the NMR spectra associated with (1) different preparative procedures; (2) different suspension media, and (3) increasing osmolality are described. The fact that high concentrations of epinephrine and ATP (approximately 700 mM total) are dissolved in the aqueous phase implies that solution phase interactions at least partially ionic in nature are responsible for the low internal osmolality of chromaffin granules in vivo. Ordered phases containing a substantial fraction of the total catecholamine in an osmotically inactive form are not present.", "contents": "Composition of the aqueous phase of chromaffin granules. Nuclear magnetic resonance spectroscopy has been used to determine the composition of the aqueous phase of bovine chromaffin granules. Relative concentrations of catecholamines (epinephrine plus norepinephrine), ATP and chromogranins have been measured from integrated intensities in the proton spectra using computer simulation techniques. Most or all of the catecholamines (97 +/- 8%) are present in the aqueous phase and contribute to the high resolution spectrum. The catecholamine:ATP molar ratio (4.41 +/- 0.45) determined by NMR is close to the value (4.45) derived from biochemical assay indicating that most or all of the ATP is present with catecholamine in the aqueous phase. Catecholamine:protein ratios show that approximately 45% of the soluble protein freed by lysis is not NMR visible. Intensity from this fraction does not appear under highly denaturing conditions (8 M urea) but reappears after hydrolysis. This behavior is similar to that of recently isolated soluble lipoprotein complexes. Variations in the NMR spectra associated with (1) different preparative procedures; (2) different suspension media, and (3) increasing osmolality are described. The fact that high concentrations of epinephrine and ATP (approximately 700 mM total) are dissolved in the aqueous phase implies that solution phase interactions at least partially ionic in nature are responsible for the low internal osmolality of chromaffin granules in vivo. Ordered phases containing a substantial fraction of the total catecholamine in an osmotically inactive form are not present."}
{"id": "PMID:486549", "title": "Studies on histamine H2 receptors coupled to cardiac adenylate cyclase. Effects of guanylnucleotides and structural requirements for agonist activity.", "content": "In particulate preparations from guinea-pig ventricle, histamine in the concentration range 10(-6)--10(-3) M caused a 3--5fold stimulation of adenylate cyclase activity which was dependent on the presence of GTP. The effects of fourteen analogs of histamine were examined on this cyclase preparation. Five of the compounds studied proved to be partial agonists relative to histamine while nine others had essentially the same intrinsic activity as histamine. The intrinsic activities of the partial agonists were increased by GppNHp to the extent that dimaprit, which was a partial agonist in the presence of GTP, became a full agonist in the presence of GppNHp. The relative potencies of the full agonists as activators of the cyclase were found to correlate with the relative potencies on physiologically defined H2 receptor systems. Activation of the cyclase by histamine, as well as by several of the agonist analogs, including dimaprit and tolazoline, was completely blocked by the H2 antagonist cimetidine, but was not affected by pharmacologically relevant concentrations of the H1 antagonist mepyramine, the beta-blocker alprenolol, or the alpha-blocker phentolamine. The results suggest that all the agonists studied probably interact with a common H2 receptor site on the cardiac muscle cell leading to activation of adenylate cyclase. The accompanying increase in cyclic AMP is presumably responsible for the chronotropic and inotropic effects of histamine and related compounds on cardiac muscle.", "contents": "Studies on histamine H2 receptors coupled to cardiac adenylate cyclase. Effects of guanylnucleotides and structural requirements for agonist activity. In particulate preparations from guinea-pig ventricle, histamine in the concentration range 10(-6)--10(-3) M caused a 3--5fold stimulation of adenylate cyclase activity which was dependent on the presence of GTP. The effects of fourteen analogs of histamine were examined on this cyclase preparation. Five of the compounds studied proved to be partial agonists relative to histamine while nine others had essentially the same intrinsic activity as histamine. The intrinsic activities of the partial agonists were increased by GppNHp to the extent that dimaprit, which was a partial agonist in the presence of GTP, became a full agonist in the presence of GppNHp. The relative potencies of the full agonists as activators of the cyclase were found to correlate with the relative potencies on physiologically defined H2 receptor systems. Activation of the cyclase by histamine, as well as by several of the agonist analogs, including dimaprit and tolazoline, was completely blocked by the H2 antagonist cimetidine, but was not affected by pharmacologically relevant concentrations of the H1 antagonist mepyramine, the beta-blocker alprenolol, or the alpha-blocker phentolamine. The results suggest that all the agonists studied probably interact with a common H2 receptor site on the cardiac muscle cell leading to activation of adenylate cyclase. The accompanying increase in cyclic AMP is presumably responsible for the chronotropic and inotropic effects of histamine and related compounds on cardiac muscle."}
{"id": "PMID:486550", "title": "Enzyme replacement via liposomes. Variations in lipid compositions determine liposomal integrity in biological fluids.", "content": "Liposomes survive exposure to biological fluids poorly, extruding trapped enzymes, drugs, or solutes upon interaction with serum or plasma constituents. We have quantified the disruptive effects of human serum on liposomes and have studied whether various modifications in their phospholipid composition might produce liposomes with an increased carrier potential for application in vivo. Multilamellar liposomes (phosphatidycholine 70:dicetyl phosphate 20:cholesterol 10) were prepared with 3H-labeled phosphatidylcholine as the lipid phase marker and [14C]inulin and horseradish peroxidase as aqueous phase markers. Gel exclusion chromatography showed that 32 +/- 3% of [14C]inulin and 27 +/- 7% of horseradish peroxidase were lost after 1 h incubation with 10% (v/v) human serum. Loss of aqueous solutes was reduced to 20 +/- 5%/h and 17 +/- 2%/h, respectively, after treatment with decomplemented serum (56 degrees C, 30 min). Loss induced by serum was concentration and time dependent: to 57 +/- 2% at 1 h and 67 +/- 14% at 24 h, with 50% serum; plasma was slightly less perturbing whereas human serum albumin was not at all disruptive. By incorporating sphingomyelin (35 mol%) into multilamellar liposomes, the leakage of [14c]-inulin in the presence of 10% serum was reduced to 12 +/- 4%/h; increasing the molar percentage of cholesterol to 35% also stabilized the lipid bilayers, reducing leakage to 20 +/- 7%/h. Both small and large unilamellar vesicles could not be stablilized against serum-mediated leakage by the incorporation of sphingomyelin. The data suggest that cholesterol and sphingomyelin enhance liposomal integrity in the presence of serum or plasma and promise to yield enhanced survival of drug-laden lipid vesicles in biological fluids in vivo.", "contents": "Enzyme replacement via liposomes. Variations in lipid compositions determine liposomal integrity in biological fluids. Liposomes survive exposure to biological fluids poorly, extruding trapped enzymes, drugs, or solutes upon interaction with serum or plasma constituents. We have quantified the disruptive effects of human serum on liposomes and have studied whether various modifications in their phospholipid composition might produce liposomes with an increased carrier potential for application in vivo. Multilamellar liposomes (phosphatidycholine 70:dicetyl phosphate 20:cholesterol 10) were prepared with 3H-labeled phosphatidylcholine as the lipid phase marker and [14C]inulin and horseradish peroxidase as aqueous phase markers. Gel exclusion chromatography showed that 32 +/- 3% of [14C]inulin and 27 +/- 7% of horseradish peroxidase were lost after 1 h incubation with 10% (v/v) human serum. Loss of aqueous solutes was reduced to 20 +/- 5%/h and 17 +/- 2%/h, respectively, after treatment with decomplemented serum (56 degrees C, 30 min). Loss induced by serum was concentration and time dependent: to 57 +/- 2% at 1 h and 67 +/- 14% at 24 h, with 50% serum; plasma was slightly less perturbing whereas human serum albumin was not at all disruptive. By incorporating sphingomyelin (35 mol%) into multilamellar liposomes, the leakage of [14c]-inulin in the presence of 10% serum was reduced to 12 +/- 4%/h; increasing the molar percentage of cholesterol to 35% also stabilized the lipid bilayers, reducing leakage to 20 +/- 7%/h. Both small and large unilamellar vesicles could not be stablilized against serum-mediated leakage by the incorporation of sphingomyelin. The data suggest that cholesterol and sphingomyelin enhance liposomal integrity in the presence of serum or plasma and promise to yield enhanced survival of drug-laden lipid vesicles in biological fluids in vivo."}
{"id": "PMID:486551", "title": "Synthesis of procollagen by odontogenic cells of rabbit tooth germ.", "content": "Studies were performed to determine whether cultured odontogenic cells from rabbit tooth germ (RP cell) could synthesize dentine-like collagen. When cells were cultured with [14C]proline, 33% of the total incorporated proteins present were collagenous. Cultured RP cells were labeled with [14C]proline in the presence of beta-aminopropionitrile. The resulting fractions, on analysis by CM-cellulose chromatography, contained three radioactive protein peaks, alpha 1(I), [alpha 1(III)]3, alpha 2. From the radioactive measurements, RP cells synthesized a significant amount of type III collagen, comparable to type I collagen. DEAE-cellulose chromatography was used to separate collagen molecules from collagen precursors. The results showed that 60% of total collagen precursor was type III precursor and the remainder was type I precursor. CM-cellulose chromatography of CNBr peptides of collagen from culture medium and cell extract revealed the presence of type I and type III collagen. Thus, the RP cell, which is a diploid cell, is unique in the predominance of type III collagen in culture, differing thereby from the character of collagen in vivo.", "contents": "Synthesis of procollagen by odontogenic cells of rabbit tooth germ. Studies were performed to determine whether cultured odontogenic cells from rabbit tooth germ (RP cell) could synthesize dentine-like collagen. When cells were cultured with [14C]proline, 33% of the total incorporated proteins present were collagenous. Cultured RP cells were labeled with [14C]proline in the presence of beta-aminopropionitrile. The resulting fractions, on analysis by CM-cellulose chromatography, contained three radioactive protein peaks, alpha 1(I), [alpha 1(III)]3, alpha 2. From the radioactive measurements, RP cells synthesized a significant amount of type III collagen, comparable to type I collagen. DEAE-cellulose chromatography was used to separate collagen molecules from collagen precursors. The results showed that 60% of total collagen precursor was type III precursor and the remainder was type I precursor. CM-cellulose chromatography of CNBr peptides of collagen from culture medium and cell extract revealed the presence of type I and type III collagen. Thus, the RP cell, which is a diploid cell, is unique in the predominance of type III collagen in culture, differing thereby from the character of collagen in vivo."}
{"id": "PMID:486552", "title": "Endocytosis and breakdown of ribonuclease oligomers by sinusoidal rat liver cells in vivo. I. Effect of size.", "content": "Aspects of protein structure determining endocytosis of proteins by sinusoidal rat liver cells in vivo have been studied, using cross-linked or aggregated derivatives of bovine pancreatic ribonuclease A (labelled with 125I) as probes. Ribonuclease was cross-linked by reaction with dimethylsuberimidate, a way of modification that does not change the charge of the protein. Monomer, dimer and polymer fractions were isolated by gel filtration and characterized in respect of size and number of amino groups modified. Maintenance of enzyme activity, stability of disulfide bonds, and lack of susceptibility to endoproteases showed that the cross-linking procedure did not result in gross conformational changes of the ribonuclease molecules. Monomer, dimer and polymer fractions were injected into nephrectomized rats and plasma clearance and uptake in liver and spleen were determined. About 30% of the injected polymer fraction was found in liver 15 min after injection; for dimer and monomer fractions values of 6% and 2% of the dose were found. Similar differences were found in spleen. Autoradiography, cell isolation, and subcellular fractionation showed that in liver the radioactive proteins were taken up in lysosomes of sinusoidal cells. Similar results were obtained with fractions of aggregated ribonuclease prepared by freeze-drying the protein from 50% acetic acid. Our results demonstrate that the rate of uptake of the ribonuclease derivatives is positively correlated with the size of the molecules. Similarity of the results obtained with cross-linked and aggregated fractions suggests that the number of ribonuclease 'subunits'/molecule, rather than the procedures used to prepare the polymers, determine the rate of uptake by liver and spleen.", "contents": "Endocytosis and breakdown of ribonuclease oligomers by sinusoidal rat liver cells in vivo. I. Effect of size. Aspects of protein structure determining endocytosis of proteins by sinusoidal rat liver cells in vivo have been studied, using cross-linked or aggregated derivatives of bovine pancreatic ribonuclease A (labelled with 125I) as probes. Ribonuclease was cross-linked by reaction with dimethylsuberimidate, a way of modification that does not change the charge of the protein. Monomer, dimer and polymer fractions were isolated by gel filtration and characterized in respect of size and number of amino groups modified. Maintenance of enzyme activity, stability of disulfide bonds, and lack of susceptibility to endoproteases showed that the cross-linking procedure did not result in gross conformational changes of the ribonuclease molecules. Monomer, dimer and polymer fractions were injected into nephrectomized rats and plasma clearance and uptake in liver and spleen were determined. About 30% of the injected polymer fraction was found in liver 15 min after injection; for dimer and monomer fractions values of 6% and 2% of the dose were found. Similar differences were found in spleen. Autoradiography, cell isolation, and subcellular fractionation showed that in liver the radioactive proteins were taken up in lysosomes of sinusoidal cells. Similar results were obtained with fractions of aggregated ribonuclease prepared by freeze-drying the protein from 50% acetic acid. Our results demonstrate that the rate of uptake of the ribonuclease derivatives is positively correlated with the size of the molecules. Similarity of the results obtained with cross-linked and aggregated fractions suggests that the number of ribonuclease 'subunits'/molecule, rather than the procedures used to prepare the polymers, determine the rate of uptake by liver and spleen."}
{"id": "PMID:486553", "title": "Endocytosis and breakdown of ribonuclease oligomers by sinusoidal rat liver cells in vivo. II. Effect of charge.", "content": "Experiments presented in this paper suggest that sinusoidal rat liver cells recognize basic groups on proteins and that this recognition results in endocytosis of the proteins. Evidence for involvement of basic groups was obtained in two ways. Firstly, we changed the positively charged amino groups of the cross-linked ribonuclease molecules to neutral or negative by acetylation or succinylation, respectively. The modified proteins did not contain easily reducible disulfide bonds and they were not very sensitive to endoproteases, suggesting that they were not denatured by the acetylation procedures. Acetylation and succinylation reduced uptake of the injected cross-linked ribonuclease derivatives by liver and spleen and abolished their rapid clearance from plasma. In nephrectomized rats about 75% of the polymer, 36% of the acetylated polymer and 32% of the succinylated polymer were endocytosed by liver after 6 h. For the dimer fractions these values were 59%, 23% and 27%, respectively. Autoradiography and subcellular fractionation of liver 30 min post-injection localized the acetylated polymer in the lysosomal/microsomal fraction of sinusoidal liver cells, probably endothelial cells. Secondly, a positive correlation was found between binding of a number of ribonuclease derivatives to the cation exchanger SP-Sephadex G-25 and the rate of endocytosis by sinusoidal liver cells.", "contents": "Endocytosis and breakdown of ribonuclease oligomers by sinusoidal rat liver cells in vivo. II. Effect of charge. Experiments presented in this paper suggest that sinusoidal rat liver cells recognize basic groups on proteins and that this recognition results in endocytosis of the proteins. Evidence for involvement of basic groups was obtained in two ways. Firstly, we changed the positively charged amino groups of the cross-linked ribonuclease molecules to neutral or negative by acetylation or succinylation, respectively. The modified proteins did not contain easily reducible disulfide bonds and they were not very sensitive to endoproteases, suggesting that they were not denatured by the acetylation procedures. Acetylation and succinylation reduced uptake of the injected cross-linked ribonuclease derivatives by liver and spleen and abolished their rapid clearance from plasma. In nephrectomized rats about 75% of the polymer, 36% of the acetylated polymer and 32% of the succinylated polymer were endocytosed by liver after 6 h. For the dimer fractions these values were 59%, 23% and 27%, respectively. Autoradiography and subcellular fractionation of liver 30 min post-injection localized the acetylated polymer in the lysosomal/microsomal fraction of sinusoidal liver cells, probably endothelial cells. Secondly, a positive correlation was found between binding of a number of ribonuclease derivatives to the cation exchanger SP-Sephadex G-25 and the rate of endocytosis by sinusoidal liver cells."}
{"id": "PMID:486554", "title": "[Effect of further complexing on the spectral-luminescent properties of Mg-porphyrins].", "content": "It has been shown by investigations of polarization spectra and the dependence of the degree of fluorescence and phosphorescence polarization on emission wavelength that in non-polar solvents at 77 degrees K the molecules of Mg porphin and Mg tetraphenylporphin possess true symmetry D4h. When polar molecules (water, alcohols, pyridine) are present in the solution further complexing of the magnesium complexes with extra-ligands takes place and the polarization data indicate that D4h symmetry is absent which leads to splitting of the doubly degenerate states by 100-150 cm-1. It has been established that on further complexing the S1 and T1 levels are lowered by approximately 500 and 700 cm-1, respectively, the probability of intercombinational degradation T1 leads to S0 increases in this case. It follows from the data obtained that the extra-ligands do not essentially influence spin-orbit interaction.", "contents": "[Effect of further complexing on the spectral-luminescent properties of Mg-porphyrins]. It has been shown by investigations of polarization spectra and the dependence of the degree of fluorescence and phosphorescence polarization on emission wavelength that in non-polar solvents at 77 degrees K the molecules of Mg porphin and Mg tetraphenylporphin possess true symmetry D4h. When polar molecules (water, alcohols, pyridine) are present in the solution further complexing of the magnesium complexes with extra-ligands takes place and the polarization data indicate that D4h symmetry is absent which leads to splitting of the doubly degenerate states by 100-150 cm-1. It has been established that on further complexing the S1 and T1 levels are lowered by approximately 500 and 700 cm-1, respectively, the probability of intercombinational degradation T1 leads to S0 increases in this case. It follows from the data obtained that the extra-ligands do not essentially influence spin-orbit interaction."}
{"id": "PMID:486556", "title": "[Temperature independence of the rate of the rapid phase of P700+ dark reduction].", "content": "To elucidate the mechanism of interaction between P700+ and reduced primary electron acceptor in the reaction centres of photosystem I dark relaxation time course of absorption changes at 700 nm was studied at different temperatures. It is shown that in photosystem I subchloroplast fragments with partially inactivated endogenous secondary electron acceptors (treatment by ether or preliminary heating) the recombination of reduced primary acceptor and P700+ is found at -15 degrees C. In fragments with undamaged system of secondary acceptors the recovery of primary photoact in darkness is observed only at temperatures lower than -95 degrees C. At temperatures from -60 degrees to -170 degrees C the electron transfer from reduced primary acceptor to P700+ is described by the first order reaction with half time 250 ms; the rate of this process does not depend on the presence of secondary electron acceptor. The temperature independence of the rate of rapid phase P700+ dark reduction is interpreted as an indication of tunneling mechanisms from reduced primary acceptor to P700+.", "contents": "[Temperature independence of the rate of the rapid phase of P700+ dark reduction]. To elucidate the mechanism of interaction between P700+ and reduced primary electron acceptor in the reaction centres of photosystem I dark relaxation time course of absorption changes at 700 nm was studied at different temperatures. It is shown that in photosystem I subchloroplast fragments with partially inactivated endogenous secondary electron acceptors (treatment by ether or preliminary heating) the recombination of reduced primary acceptor and P700+ is found at -15 degrees C. In fragments with undamaged system of secondary acceptors the recovery of primary photoact in darkness is observed only at temperatures lower than -95 degrees C. At temperatures from -60 degrees to -170 degrees C the electron transfer from reduced primary acceptor to P700+ is described by the first order reaction with half time 250 ms; the rate of this process does not depend on the presence of secondary electron acceptor. The temperature independence of the rate of rapid phase P700+ dark reduction is interpreted as an indication of tunneling mechanisms from reduced primary acceptor to P700+."}
{"id": "PMID:486559", "title": "[Quasithermal crystallization of cholesteric mixtures].", "content": "Behaviour of cholesteric mixtures transiting into isotropic state at 30-38 degrees C kept at 20 degrees C for a long time was studied. It is found that besides transitions from a flat to cylindrical texture concerned with the defects in the sample, crystallization of the mixtures according to the dislocation mechanism is possible. The critical size of crystallization nuclei was estimated. The discovered phenomenon of dislocation growth of crystals from the cholesteric mixture is important for studying the mechanisms of crystallic phase isolation from cholesteric mixtures and for understanding of the crystallization processes in biological liquid crystal systems.", "contents": "[Quasithermal crystallization of cholesteric mixtures]. Behaviour of cholesteric mixtures transiting into isotropic state at 30-38 degrees C kept at 20 degrees C for a long time was studied. It is found that besides transitions from a flat to cylindrical texture concerned with the defects in the sample, crystallization of the mixtures according to the dislocation mechanism is possible. The critical size of crystallization nuclei was estimated. The discovered phenomenon of dislocation growth of crystals from the cholesteric mixture is important for studying the mechanisms of crystallic phase isolation from cholesteric mixtures and for understanding of the crystallization processes in biological liquid crystal systems."}
{"id": "PMID:486560", "title": "[Study of the molecular structure and mechanisms of pond snail neuron cholinoreceptor function by means of in vivo chemical modification].", "content": "The molecular structure of acetylcholine receptors (AChR) of Lymnaea stagnalis neurons has been studied using specific agents to definite chemical groups. The disulphide bond important for AChR function was discovered, the reduction of which by dithiotreitol (DTT) inactivates AChR. The drugs exciting AChR protect the disulphide bond against modification with DTT likely due to the conformational changes of an active site and its environment. Desensitized AChR can also be modified by DDT (if it is not occupied by agonist). It is suggested that the system transmitting the conformational change from the AChR active site to its ionophore is responsible for desensitization.", "contents": "[Study of the molecular structure and mechanisms of pond snail neuron cholinoreceptor function by means of in vivo chemical modification]. The molecular structure of acetylcholine receptors (AChR) of Lymnaea stagnalis neurons has been studied using specific agents to definite chemical groups. The disulphide bond important for AChR function was discovered, the reduction of which by dithiotreitol (DTT) inactivates AChR. The drugs exciting AChR protect the disulphide bond against modification with DTT likely due to the conformational changes of an active site and its environment. Desensitized AChR can also be modified by DDT (if it is not occupied by agonist). It is suggested that the system transmitting the conformational change from the AChR active site to its ionophore is responsible for desensitization."}
{"id": "PMID:486562", "title": "[Possibility of hydromagnetic wave excitation in a physiologic aqueous solution].", "content": "Oscillation processes of Cl- and Na+ ions (the latter form aquacomplexes with 6 water molecules) existing in a constant geomagnetic field are analysed in NaCl aqueous solution. Joint solution of hydro- and electrodynamic equations gives several wave types. Hydromagnetic waves of two modes show the frequency dispersion of phase velocities. This results in the existence of two maxima of oscillation velicity in the frequencies 2-6 Hz and 20-30 Hz close to the cyclotron frequencies of ions. The hydromagnetic waves can have a large amplitude only when there is a variable disturbing force, e.g. a variable component of the magnetic field at the background of the constant field.", "contents": "[Possibility of hydromagnetic wave excitation in a physiologic aqueous solution]. Oscillation processes of Cl- and Na+ ions (the latter form aquacomplexes with 6 water molecules) existing in a constant geomagnetic field are analysed in NaCl aqueous solution. Joint solution of hydro- and electrodynamic equations gives several wave types. Hydromagnetic waves of two modes show the frequency dispersion of phase velocities. This results in the existence of two maxima of oscillation velicity in the frequencies 2-6 Hz and 20-30 Hz close to the cyclotron frequencies of ions. The hydromagnetic waves can have a large amplitude only when there is a variable disturbing force, e.g. a variable component of the magnetic field at the background of the constant field."}
{"id": "PMID:486561", "title": "[Relationship between unidirectional sodium ion currents and their concentration in muscle and medium].", "content": "Sodium fluxes were measured as concentrations of external sodium and intracellular sodium were changed under K-free condition. Paired sartorious muscles with high intracellular sodium concentration from frog (Rana ridibunda) were used throughout this investigation. The measured uptake of 22Na (mM/kg . h) is directly proportional to intracellular sodium but when influx of 22Na is calculated per unit surface (pmole/cm2s) taking account of \"effect of relation volume/surface\" the influx is to be independent of Nain. Sodium influx varied linearly with changing external Na. Sodium efflux is not dependent on external sodium concentration. The results are in favour of the conclusion that the sodium fluxes across the skeletal muscle membrane do not exhibit the mechanism of sodium--sodium exchange.", "contents": "[Relationship between unidirectional sodium ion currents and their concentration in muscle and medium]. Sodium fluxes were measured as concentrations of external sodium and intracellular sodium were changed under K-free condition. Paired sartorious muscles with high intracellular sodium concentration from frog (Rana ridibunda) were used throughout this investigation. The measured uptake of 22Na (mM/kg . h) is directly proportional to intracellular sodium but when influx of 22Na is calculated per unit surface (pmole/cm2s) taking account of \"effect of relation volume/surface\" the influx is to be independent of Nain. Sodium influx varied linearly with changing external Na. Sodium efflux is not dependent on external sodium concentration. The results are in favour of the conclusion that the sodium fluxes across the skeletal muscle membrane do not exhibit the mechanism of sodium--sodium exchange."}
{"id": "PMID:486563", "title": "[Increase in the rigidity of bioconstruction material during the process of morphogenesis].", "content": "Structural rigidity of the material of cell wall in the cylindrical part of Acetabularia apex resulting from the fibrillar structure and of the cell wall properties and deformations realized during anisotropy, were studied. Values of Jung's moduli and Poisson's coefficient of the growing wall were obtained. It was shown that anisotropy of the material featured inner geometrical rigidity of the bioconstruction which restricted the deformations, and in case of structural instability predetermined their morphogenetic switching.", "contents": "[Increase in the rigidity of bioconstruction material during the process of morphogenesis]. Structural rigidity of the material of cell wall in the cylindrical part of Acetabularia apex resulting from the fibrillar structure and of the cell wall properties and deformations realized during anisotropy, were studied. Values of Jung's moduli and Poisson's coefficient of the growing wall were obtained. It was shown that anisotropy of the material featured inner geometrical rigidity of the bioconstruction which restricted the deformations, and in case of structural instability predetermined their morphogenetic switching."}
{"id": "PMID:486565", "title": "[Model of physiological thermoregulation].", "content": "Temperature dependence of energy transformations in the cell being considered as the basis of thermoregulatory reactions in any organism, the mathematical model of energy obtaining and utilization of some formal system which can be considered as a cell prototype is composed. The mode is realized in the form of differential equations which describe the energy balance in the dynamic system and the energy exchange between the system and the environment. The steady state of the system structural elements is interpreted as a \"hypothetic\" aim of the regulation. Temperature \"set interval\" and \"non-thermal\" factors role in the formation of the last is based on the equations solutions which correspond to quasi-stationary states of the system. The model can be used as an element of mathematical description of organism thermoregulation. A scheme of possible synthesis of organism of thermoregulation model is considered.", "contents": "[Model of physiological thermoregulation]. Temperature dependence of energy transformations in the cell being considered as the basis of thermoregulatory reactions in any organism, the mathematical model of energy obtaining and utilization of some formal system which can be considered as a cell prototype is composed. The mode is realized in the form of differential equations which describe the energy balance in the dynamic system and the energy exchange between the system and the environment. The steady state of the system structural elements is interpreted as a \"hypothetic\" aim of the regulation. Temperature \"set interval\" and \"non-thermal\" factors role in the formation of the last is based on the equations solutions which correspond to quasi-stationary states of the system. The model can be used as an element of mathematical description of organism thermoregulation. A scheme of possible synthesis of organism of thermoregulation model is considered."}
{"id": "PMID:486568", "title": "[Nerve impulse conduction along myelinated fibers while internodal vary (mathematical model)].", "content": "The mathematical model of a myelinated fibre (Hille, 1971) was used to study the dependence of the velocity of nerve impulse propagation (theta) and of some parameters of the action potential on the properties of internodes. Calculations have shown that with increasing of the length (L) of internodes over the range of 0.75-3 mm, theta rises and then declines; in the fibre with the external diameter, D = 14 mu the maximum of theta falls on L = 1.5 mm. With decreasing of d/D (d = internal diameter of the fibre) at expense of D (simulation of the myelin sheath thickening) theta grows up monotonically, while the safety factor N (defined as the ratio of the potential (V) in the 6th node to V in the 8th node at a moment when V in the 8th node reaches its maximum) rises steeply only up to d/D approximately 0.75; with further increasing of D, N increases insignificantly. The raising of the longitudinal resistance (ri + r0) leads to the gradual decrease of theta; at ri + r0 = 70 mohm/cm the nerve impulse propagation ceased. The estimation of the longitudinal resistance of the intercellular clefts suggests that in the nerve trunks with a compact packing of nerve fibres the flow of the local currents through the axoplasm of the neighbouring fibres is a prerequisite for impulse conduction. The possibility of electrical (electronical) interaction between the membranes of nodes and internodes has been studied. Calculations have shown that if the generation of a membrane potential in the internode were absent, the resting potential of the node would by 10 mv lower than the potential created by the nodal \"generator\".", "contents": "[Nerve impulse conduction along myelinated fibers while internodal vary (mathematical model)]. The mathematical model of a myelinated fibre (Hille, 1971) was used to study the dependence of the velocity of nerve impulse propagation (theta) and of some parameters of the action potential on the properties of internodes. Calculations have shown that with increasing of the length (L) of internodes over the range of 0.75-3 mm, theta rises and then declines; in the fibre with the external diameter, D = 14 mu the maximum of theta falls on L = 1.5 mm. With decreasing of d/D (d = internal diameter of the fibre) at expense of D (simulation of the myelin sheath thickening) theta grows up monotonically, while the safety factor N (defined as the ratio of the potential (V) in the 6th node to V in the 8th node at a moment when V in the 8th node reaches its maximum) rises steeply only up to d/D approximately 0.75; with further increasing of D, N increases insignificantly. The raising of the longitudinal resistance (ri + r0) leads to the gradual decrease of theta; at ri + r0 = 70 mohm/cm the nerve impulse propagation ceased. The estimation of the longitudinal resistance of the intercellular clefts suggests that in the nerve trunks with a compact packing of nerve fibres the flow of the local currents through the axoplasm of the neighbouring fibres is a prerequisite for impulse conduction. The possibility of electrical (electronical) interaction between the membranes of nodes and internodes has been studied. Calculations have shown that if the generation of a membrane potential in the internode were absent, the resting potential of the node would by 10 mv lower than the potential created by the nodal \"generator\"."}
{"id": "PMID:486576", "title": "Role of glucocorticoids and progesterone in the development of rough endoplasmic reticulum involved in casein biosynthesis.", "content": "Hydrocortisone acetate injected into pseudopregnant rabbits induced casein synthesis and a parallel accumulation of casein mRNA. These effects were not accompanied by any enrichment of total RNA in the mammary cell. Hydrocortisone acetate did not favour the attachment of polysomes to endoplasmic reticulum. Casein mRNA concentration was enhanced in free and membrane-bound polysomes. After long treatments, the concentration of casein mRNA reached a plateau in membrane bound polysomes whereas it continued to be accumulated in free polysomes, suggesting that a substantial part of casein synthesis is then carried out by free polysomes. Progesterone injected with high doses of prolactin was unable to prevent the stimulatory action of prolactin on the synthesis of casein, the accumulation of casein mRNA and mammary gland growth, as judged by DNA content. By contrast, the increase in the total RNA content of mammary gland was still significantly reduced by progesterone. In addition, progesterone inhibited almost completely the formation of membrane-bound polysomes and the anchorage of casein mRNA to endoplasmic reticulum. From these data, it was concluded that the formation of the endoplasmic reticulum is not a prerequisite for the initiation of casein synthesis. Glucocorticoids do not play a major role in the formation of the endoplasmic reticulum and the Golai apparatus and in the binding of casein synthesizing polysomes to membranes. Progesteronne is capable of inhibiting preferentially and gradually the stimulation of cellular functions requiring the most potent prolactin stimulation.", "contents": "Role of glucocorticoids and progesterone in the development of rough endoplasmic reticulum involved in casein biosynthesis. Hydrocortisone acetate injected into pseudopregnant rabbits induced casein synthesis and a parallel accumulation of casein mRNA. These effects were not accompanied by any enrichment of total RNA in the mammary cell. Hydrocortisone acetate did not favour the attachment of polysomes to endoplasmic reticulum. Casein mRNA concentration was enhanced in free and membrane-bound polysomes. After long treatments, the concentration of casein mRNA reached a plateau in membrane bound polysomes whereas it continued to be accumulated in free polysomes, suggesting that a substantial part of casein synthesis is then carried out by free polysomes. Progesterone injected with high doses of prolactin was unable to prevent the stimulatory action of prolactin on the synthesis of casein, the accumulation of casein mRNA and mammary gland growth, as judged by DNA content. By contrast, the increase in the total RNA content of mammary gland was still significantly reduced by progesterone. In addition, progesterone inhibited almost completely the formation of membrane-bound polysomes and the anchorage of casein mRNA to endoplasmic reticulum. From these data, it was concluded that the formation of the endoplasmic reticulum is not a prerequisite for the initiation of casein synthesis. Glucocorticoids do not play a major role in the formation of the endoplasmic reticulum and the Golai apparatus and in the binding of casein synthesizing polysomes to membranes. Progesteronne is capable of inhibiting preferentially and gradually the stimulation of cellular functions requiring the most potent prolactin stimulation."}
{"id": "PMID:486577", "title": "Regulation of plasminogen activator secretion in mouse peritoneal macrophages. I. - Role of serum studied by a new spectrophotometric assay for plasminogen activators.", "content": "A chromogenic tripeptide - H-D-Val-Leu-Lys-p-nitroanilide-substrate of plasmin, can be used to follow plasminogen activation by an activator such as urokinase or the activator secreted by mouse peritoneal macrophages (thioglycolate-elicited). The acceleration of p-nitroaniline production is proportional to the initial rate of plasmin formation from plasminogen. Thus, at a given plasminogen concentration, this acceleration is proportional to the activator concentration. The acceleration can be evaluated from the spectrophotometer trace recording at 405 nm the appearance of p-nitroaniline, either by means of a computer program or by a plot of delta A405 vs.t2. The sensitivity of this assay allows detection of 0.003 CTA units of urokinase. Thioglycollate-elicited mouse peritoneal macrophages secrete plasminogen activator into the extracellular medium during in vitro cultivation only after a contact with serum.", "contents": "Regulation of plasminogen activator secretion in mouse peritoneal macrophages. I. - Role of serum studied by a new spectrophotometric assay for plasminogen activators. A chromogenic tripeptide - H-D-Val-Leu-Lys-p-nitroanilide-substrate of plasmin, can be used to follow plasminogen activation by an activator such as urokinase or the activator secreted by mouse peritoneal macrophages (thioglycolate-elicited). The acceleration of p-nitroaniline production is proportional to the initial rate of plasmin formation from plasminogen. Thus, at a given plasminogen concentration, this acceleration is proportional to the activator concentration. The acceleration can be evaluated from the spectrophotometer trace recording at 405 nm the appearance of p-nitroaniline, either by means of a computer program or by a plot of delta A405 vs.t2. The sensitivity of this assay allows detection of 0.003 CTA units of urokinase. Thioglycollate-elicited mouse peritoneal macrophages secrete plasminogen activator into the extracellular medium during in vitro cultivation only after a contact with serum."}
{"id": "PMID:486566", "title": "[Spatially heterogeneous distribution of phytoplankton in a model of a regulated population].", "content": "With the help of the mathematical model is shown that the existence of the phitoplankton's mechanism of growth-speed regulation by means of excretion to the environment of biologically active substances, may lead to a considerable inhomogenity of it's spatial distribution, that is, patchness. There appears an intensive crowding of phitoplankton with homogenious distribution of nutrients, while some limitations of excretion rate and of dispersion coefficients are taken into account.", "contents": "[Spatially heterogeneous distribution of phytoplankton in a model of a regulated population]. With the help of the mathematical model is shown that the existence of the phitoplankton's mechanism of growth-speed regulation by means of excretion to the environment of biologically active substances, may lead to a considerable inhomogenity of it's spatial distribution, that is, patchness. There appears an intensive crowding of phitoplankton with homogenious distribution of nutrients, while some limitations of excretion rate and of dispersion coefficients are taken into account."}
{"id": "PMID:486578", "title": "General and lysin specific control of saccharopine dehydrogenase levels in the yeast Saccharomycopsis lipolytica.", "content": "Lysine supplementation of the growth medium of a wild type strain of the yeast Saccharomycopsis lipolytica specifically results in saccharopine dehydrogenase repression. Starvation of the strain for histidine triggers a general depression of various histidine, leucine, arginine and lysine biosynthetic enzymes, including saccharopine dehydrogenase. These two types of control, specific and general, act independently on saccharopine dehydrogenase expression, since mutants which fail to respond to the specific control still are sensitive to the general one. These mutants were first selected as unable to catabolize lysine, suggesting that a link may exist between saccharopine dehydrogenase specific regulation and activity of the catabolic pathway.", "contents": "General and lysin specific control of saccharopine dehydrogenase levels in the yeast Saccharomycopsis lipolytica. Lysine supplementation of the growth medium of a wild type strain of the yeast Saccharomycopsis lipolytica specifically results in saccharopine dehydrogenase repression. Starvation of the strain for histidine triggers a general depression of various histidine, leucine, arginine and lysine biosynthetic enzymes, including saccharopine dehydrogenase. These two types of control, specific and general, act independently on saccharopine dehydrogenase expression, since mutants which fail to respond to the specific control still are sensitive to the general one. These mutants were first selected as unable to catabolize lysine, suggesting that a link may exist between saccharopine dehydrogenase specific regulation and activity of the catabolic pathway."}
{"id": "PMID:486573", "title": "[Properties of bilayer lipid membranes formed from cholesterol and alkyltrimethylammonium bromides].", "content": "Flat bilayer lipid membranes (BLM) were obtained from cholesterol solution in n-octane in the presence of detergents of alkyltrimethyl ammonium bromides series in water medium. It has been shown that highly stable BLM are obtained only for the detergents of this series with the alkyl chain higher than C12. Changes in stability and electromechanical strength of the latter are conditioned by the peculiarities of stechiometric interaction between the molecules of cholesterol, detergent and solvent in the lipid bilayer.", "contents": "[Properties of bilayer lipid membranes formed from cholesterol and alkyltrimethylammonium bromides]. Flat bilayer lipid membranes (BLM) were obtained from cholesterol solution in n-octane in the presence of detergents of alkyltrimethyl ammonium bromides series in water medium. It has been shown that highly stable BLM are obtained only for the detergents of this series with the alkyl chain higher than C12. Changes in stability and electromechanical strength of the latter are conditioned by the peculiarities of stechiometric interaction between the molecules of cholesterol, detergent and solvent in the lipid bilayer."}
{"id": "PMID:486574", "title": "[Localization of water in DNA molecules by the difference Fourier-synthesis method].", "content": "Coordinates of water molecules for B-form of DNA (19 molecules) were determined by the methods of Fourier- and difference Fourier-synthesis. There was shown the strong hydration of the major groove in comparison with the minor one; and one may suppose the different hydration mechanisms for two grooves. There was found water following symmetry of DNA itself and water transforming by the symmetry of cell. Some assumption were made on the different functional properties of grooves of DNA in connection with hydration and also on the character of water dynamics.", "contents": "[Localization of water in DNA molecules by the difference Fourier-synthesis method]. Coordinates of water molecules for B-form of DNA (19 molecules) were determined by the methods of Fourier- and difference Fourier-synthesis. There was shown the strong hydration of the major groove in comparison with the minor one; and one may suppose the different hydration mechanisms for two grooves. There was found water following symmetry of DNA itself and water transforming by the symmetry of cell. Some assumption were made on the different functional properties of grooves of DNA in connection with hydration and also on the character of water dynamics."}
{"id": "PMID:486580", "title": "[Influence of phosphatidylcholine/cholesterol molar ratios in liposomes on cholesterol reactivity with cholesterol:oxygen oxidoreductase].", "content": "The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosphatidylcholines nature and the phosphatidylcholine/cholesterol molar ratio (R), the enzymatic oxidation rate of liposomal cholesterol is slower than when the reaction is developed in the present of Thesit, a surfactif agent which destroyes the lamellar particles. This is true whether Thesit is added during preparation of dispersions or during incubation with cholesterol oxydase. The enzymatic oxydation rate of cholesterol of ternary systems phosphatidylcholine-cholesterol-Thesit is independent of the (R) value and the phosphatidylcholine fatty acid unsaturation, whereas that of phosphatidylcholine-cholesterol dispersions depends on these two parameters. The reaction rate increases in the order: dipalmitoylphosphatidylcholine to yolk egg phosphatidylcholines, and dioleylphosphatidylcholine. The optimal conditions for cholesterol oxidation were found to be R = 0.5. This result is not affected by the phosphatidylcholines nature. In order to explain these data, various hypotheses are considered. In particular, the weak liposomal cholesterol reactivity with cholesterol oxidase could result from an inhibitory effect on the enzyme-substrate combination due to the polar phosphorylcholine groups.", "contents": "[Influence of phosphatidylcholine/cholesterol molar ratios in liposomes on cholesterol reactivity with cholesterol:oxygen oxidoreductase]. The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosphatidylcholines nature and the phosphatidylcholine/cholesterol molar ratio (R), the enzymatic oxidation rate of liposomal cholesterol is slower than when the reaction is developed in the present of Thesit, a surfactif agent which destroyes the lamellar particles. This is true whether Thesit is added during preparation of dispersions or during incubation with cholesterol oxydase. The enzymatic oxydation rate of cholesterol of ternary systems phosphatidylcholine-cholesterol-Thesit is independent of the (R) value and the phosphatidylcholine fatty acid unsaturation, whereas that of phosphatidylcholine-cholesterol dispersions depends on these two parameters. The reaction rate increases in the order: dipalmitoylphosphatidylcholine to yolk egg phosphatidylcholines, and dioleylphosphatidylcholine. The optimal conditions for cholesterol oxidation were found to be R = 0.5. This result is not affected by the phosphatidylcholines nature. In order to explain these data, various hypotheses are considered. In particular, the weak liposomal cholesterol reactivity with cholesterol oxidase could result from an inhibitory effect on the enzyme-substrate combination due to the polar phosphorylcholine groups."}
{"id": "PMID:486581", "title": "[Partial characterization of 2 extracellular aminopeptidases (arylamidases) from the dermatophyte Keratinomyces ajelloi].", "content": "Two aminopeptidases AP1 and AP2 have been isolated from Keratinomyces ajelloi filtrates. The molecular weight is about 27 000 for AP1 and 23 000 for AP2. Both aminopeptidases present maximum activity at pH 9.35 but 50 p. 100 of maximum activity is observed between pH 7.5 and pH 8.5. Km values measured at pH 9.35 with L-leucine-p-nitroanilide as substrate are 0.38 X 10(-3) M for AP1 and 0.43 X 10(-3) M for AP2. kcat at the same pH are 63.6 sec.-1 for AP-1 and 62.8 sec-1 for AP2. Both aminopeptidases are inhibited by mercuric chloride, o-phenanthroline, dithiothreitol and 2-mercaptoethanol. Some of their characters make them similar to Streptomyces griseus pronase aminopeptidases.", "contents": "[Partial characterization of 2 extracellular aminopeptidases (arylamidases) from the dermatophyte Keratinomyces ajelloi]. Two aminopeptidases AP1 and AP2 have been isolated from Keratinomyces ajelloi filtrates. The molecular weight is about 27 000 for AP1 and 23 000 for AP2. Both aminopeptidases present maximum activity at pH 9.35 but 50 p. 100 of maximum activity is observed between pH 7.5 and pH 8.5. Km values measured at pH 9.35 with L-leucine-p-nitroanilide as substrate are 0.38 X 10(-3) M for AP1 and 0.43 X 10(-3) M for AP2. kcat at the same pH are 63.6 sec.-1 for AP-1 and 62.8 sec-1 for AP2. Both aminopeptidases are inhibited by mercuric chloride, o-phenanthroline, dithiothreitol and 2-mercaptoethanol. Some of their characters make them similar to Streptomyces griseus pronase aminopeptidases."}
{"id": "PMID:486582", "title": "Study of fluorescent tryptophyl residues and extrinsic probes for the characterization of molecular domains of Folch-Pi apoprotein.", "content": "The highly hydrophobic myelin Folch-Pi apoprotein can be solubilized in organic as well as in aqueous media. In order to understand the molecular organization changes consecutive to changes in the solvent medium, the environment of intrinsic probes and extrinsic labels has been studied by fluorescence and accessibility to some reagents. In acqueous solution, only two tryptophan residues per protein molecule of 23,500 molecular weight have been shown to fluoresce, and their fluorescence characterisitics indicate an hydrophobic and/or constrained environment. Two ANS binding sites have also been observed having a high quenching effect on the intrinsic chromophore fluorescence. A large accessibility has been evidenced for the protein sulfhydryl groups in chloroform-methanol 2:1 (v/v), both by kinetic study of the protein reaction with a specific reagent, N-(1-anilino-naphtyl-4) maleimide, and by the fluorescence characteristics of this probe once linked to the protein. The free sulfhydryl groups were still reactive in acqueous solution, but extrinsic fluorescence of the labelled apoprotein transferred from chloroform-methanol 2:1 (v/v) into water gave evidence of constraints on the probe or on its environment. Such constraints may contribute to the solubilization in acqueous solution of this highly hydrophobic protein.", "contents": "Study of fluorescent tryptophyl residues and extrinsic probes for the characterization of molecular domains of Folch-Pi apoprotein. The highly hydrophobic myelin Folch-Pi apoprotein can be solubilized in organic as well as in aqueous media. In order to understand the molecular organization changes consecutive to changes in the solvent medium, the environment of intrinsic probes and extrinsic labels has been studied by fluorescence and accessibility to some reagents. In acqueous solution, only two tryptophan residues per protein molecule of 23,500 molecular weight have been shown to fluoresce, and their fluorescence characterisitics indicate an hydrophobic and/or constrained environment. Two ANS binding sites have also been observed having a high quenching effect on the intrinsic chromophore fluorescence. A large accessibility has been evidenced for the protein sulfhydryl groups in chloroform-methanol 2:1 (v/v), both by kinetic study of the protein reaction with a specific reagent, N-(1-anilino-naphtyl-4) maleimide, and by the fluorescence characteristics of this probe once linked to the protein. The free sulfhydryl groups were still reactive in acqueous solution, but extrinsic fluorescence of the labelled apoprotein transferred from chloroform-methanol 2:1 (v/v) into water gave evidence of constraints on the probe or on its environment. Such constraints may contribute to the solubilization in acqueous solution of this highly hydrophobic protein."}
{"id": "PMID:486583", "title": "On the degradation of heparin by human blood platelets.", "content": "Human blood platelets are able to degrade heparin from different tissues and species. The main degradation product is an oligosaccharide. Low molecular weight components such as inorganic sulfate or monosaccharides, i.e. products released by exoenzymes are not detected. The in vitro degradation of heparin by the crude enzyme is observed at pHs below 6.5 with an optimum temperature around 37 degrees C. The presence of sulfate in the substrate structure is shown to be essential for the enzyme activity. Since the oligosaccharides formed have only 10 per cent of the anticoagulant activity of the heparins tested, it is conceivable that the platelet enzyme may play an important role in the inactivation of some of the biological properties of heparin.", "contents": "On the degradation of heparin by human blood platelets. Human blood platelets are able to degrade heparin from different tissues and species. The main degradation product is an oligosaccharide. Low molecular weight components such as inorganic sulfate or monosaccharides, i.e. products released by exoenzymes are not detected. The in vitro degradation of heparin by the crude enzyme is observed at pHs below 6.5 with an optimum temperature around 37 degrees C. The presence of sulfate in the substrate structure is shown to be essential for the enzyme activity. Since the oligosaccharides formed have only 10 per cent of the anticoagulant activity of the heparins tested, it is conceivable that the platelet enzyme may play an important role in the inactivation of some of the biological properties of heparin."}
{"id": "PMID:486584", "title": "[Zein of maize grain. Preparation and characterization].", "content": "A laboratory procedure for isolation and purification of zein from grains of 4 varieties of Maize was described. The preparations were characterized by their physicochemical properties. Upon polyacrylamide gel electrophoresis in sodium dodecyl sulphate (SDS), native zein (from INRA 260 hybrid) was resolved into 2 major classes with average molecular weights of 45,000 and 22,000. After reduction with mercaptoethanol zein contained only two subunits of 22,000 and 24,000 daltons. Upon starch gel electrophoresis in 6 M urea at pH 3.5, native zein exhibited five major or medium intensity bands and several minor ones. The latter, under reducing conditions, disappeared to reinforce the major bands or to yield some new minor bands. Amino acid analysis revealed a very low content of lysine. The NH2-terminal amino acids were determined to be threonine and phenylalanine with a preponderance of the former. Zeins isolated from the varieties studied appeared tohave the same NH2-terminal residues and similar amino acid compositions with an arginine/histidine ratio ranging from 1.1 to 1.2. They differed in relative importance of components, detected by electrophoresis in the presence of SDS or urea. Changes in zein characteristics with the grain genotype allow one to conclude that the components of molecular weights of 22,000 and 24,000 consist of several subunits differing in charge and amino acid content.", "contents": "[Zein of maize grain. Preparation and characterization]. A laboratory procedure for isolation and purification of zein from grains of 4 varieties of Maize was described. The preparations were characterized by their physicochemical properties. Upon polyacrylamide gel electrophoresis in sodium dodecyl sulphate (SDS), native zein (from INRA 260 hybrid) was resolved into 2 major classes with average molecular weights of 45,000 and 22,000. After reduction with mercaptoethanol zein contained only two subunits of 22,000 and 24,000 daltons. Upon starch gel electrophoresis in 6 M urea at pH 3.5, native zein exhibited five major or medium intensity bands and several minor ones. The latter, under reducing conditions, disappeared to reinforce the major bands or to yield some new minor bands. Amino acid analysis revealed a very low content of lysine. The NH2-terminal amino acids were determined to be threonine and phenylalanine with a preponderance of the former. Zeins isolated from the varieties studied appeared tohave the same NH2-terminal residues and similar amino acid compositions with an arginine/histidine ratio ranging from 1.1 to 1.2. They differed in relative importance of components, detected by electrophoresis in the presence of SDS or urea. Changes in zein characteristics with the grain genotype allow one to conclude that the components of molecular weights of 22,000 and 24,000 consist of several subunits differing in charge and amino acid content."}
{"id": "PMID:486586", "title": "Biofeedback treatment of atopic dermatitis: controlled case studies of eight cases.", "content": "To investigate the feasibility of a behaviorally oriented intervention program with atopic dermatitis, 12 patients were exposed to a fixed sequence of treatment phases including a no-treatment baseline phase, a phase incorporating nonspecific treatment factors, and a phase involving frontal electromyographic (EMG) feedback and relaxation instructions. Photographic analyses of involved skin areas revealed significant remission of dermatological problems across the entire program, although significant changes could not be attributable to any specific phase. Ratings of itching level decreased within but not across treatment sessions, and variable correlations across subjects were found between frontal EMG and itching level. MMPI results from the dermatitis subjects were within normal limits. Overall, the results provided mixed support for the hypothesis that atopic dermatitis may be amenable to intervention through behaviorally oriented treatment procedures.", "contents": "Biofeedback treatment of atopic dermatitis: controlled case studies of eight cases. To investigate the feasibility of a behaviorally oriented intervention program with atopic dermatitis, 12 patients were exposed to a fixed sequence of treatment phases including a no-treatment baseline phase, a phase incorporating nonspecific treatment factors, and a phase involving frontal electromyographic (EMG) feedback and relaxation instructions. Photographic analyses of involved skin areas revealed significant remission of dermatological problems across the entire program, although significant changes could not be attributable to any specific phase. Ratings of itching level decreased within but not across treatment sessions, and variable correlations across subjects were found between frontal EMG and itching level. MMPI results from the dermatitis subjects were within normal limits. Overall, the results provided mixed support for the hypothesis that atopic dermatitis may be amenable to intervention through behaviorally oriented treatment procedures."}
{"id": "PMID:486587", "title": "Feedback control of human alpha rhythm from the central area.", "content": "Twenty subjects, aged 17 to 25, were given from 5 to 10 sessions of training in controlling alpha. They were divided into three groups, respectively reinforced for increasing alpha from the central area, reinforced for decreasing alpha from the central area, and given noncontingent reinforcement. Compared with the initial baseline, the alpha of the noncontingent subjects did not change, while those reinforced for increases were reliably higher and those reinforced for decreases reliably lower than the noncontingent group. A slight trend toward improvement during successive sessions was not reliable. Since the experiment was conducted in the Soviet Union, the subjects had no expectations of an \"alpha experience.\" Although tests showed a slight elevation in mood at the end of the sessions, there were no differences among the groups. There was an increase in reports of fatigue after the training sessions. There were no reports of using visual or somatomotor maneuvers as a means of controlling alpha. Furthermore, alpha rhythm control was not found to be consistently correlated with changes in heart rate, respiration, or mood, as determined by cross-correlation analysis.", "contents": "Feedback control of human alpha rhythm from the central area. Twenty subjects, aged 17 to 25, were given from 5 to 10 sessions of training in controlling alpha. They were divided into three groups, respectively reinforced for increasing alpha from the central area, reinforced for decreasing alpha from the central area, and given noncontingent reinforcement. Compared with the initial baseline, the alpha of the noncontingent subjects did not change, while those reinforced for increases were reliably higher and those reinforced for decreases reliably lower than the noncontingent group. A slight trend toward improvement during successive sessions was not reliable. Since the experiment was conducted in the Soviet Union, the subjects had no expectations of an \"alpha experience.\" Although tests showed a slight elevation in mood at the end of the sessions, there were no differences among the groups. There was an increase in reports of fatigue after the training sessions. There were no reports of using visual or somatomotor maneuvers as a means of controlling alpha. Furthermore, alpha rhythm control was not found to be consistently correlated with changes in heart rate, respiration, or mood, as determined by cross-correlation analysis."}
{"id": "PMID:486588", "title": "Partial reinforcement in human biofeedback learning.", "content": "This paper reviews the evidence for the efficacy of partial reinforcement in producing resistance to extinction in human biofeedback experiments. The methodological criteria necessary to demonstrate such effects are discussed, as is the status of the analogy of reinforcement and information feedback. It is suggested that the problem of maintaining responding in the absence of feedback should be tackled empirically rather than assuming the validity of findings from other areas of learning theory.", "contents": "Partial reinforcement in human biofeedback learning. This paper reviews the evidence for the efficacy of partial reinforcement in producing resistance to extinction in human biofeedback experiments. The methodological criteria necessary to demonstrate such effects are discussed, as is the status of the analogy of reinforcement and information feedback. It is suggested that the problem of maintaining responding in the absence of feedback should be tackled empirically rather than assuming the validity of findings from other areas of learning theory."}
{"id": "PMID:486589", "title": "The use of EEG theta biofeedback in the treatment of a patient with sleep-onset insomnia.", "content": "In this report, the treatment of a 42-year-old female with a complaint of chronic sleep-onset insomnia is described. Following the unsuccessful use of relaxation training, treatment consisted of 11 sessions of EEG theta rhythm (4--7 Hz) biofeedback. Theta density and five sleep indices were monitored throughout baseline, placebo, and treatment sessions. A significant increase in theta density was accompanied by reports of a decrease in sleep latency and an increase in total sleep time. This improvement was maintained after withdrawal of medication and at 3-month follow-up.", "contents": "The use of EEG theta biofeedback in the treatment of a patient with sleep-onset insomnia. In this report, the treatment of a 42-year-old female with a complaint of chronic sleep-onset insomnia is described. Following the unsuccessful use of relaxation training, treatment consisted of 11 sessions of EEG theta rhythm (4--7 Hz) biofeedback. Theta density and five sleep indices were monitored throughout baseline, placebo, and treatment sessions. A significant increase in theta density was accompanied by reports of a decrease in sleep latency and an increase in total sleep time. This improvement was maintained after withdrawal of medication and at 3-month follow-up."}
{"id": "PMID:486590", "title": "Combined dynamics of EEG and evoked potentials. II. Studies of simultaneously recorded EEG-EPograms in the auditory pathway, reticular formation, and hippocampus of the cat brain during sleep.", "content": "This study is carried out on single (not averaged) recordings combining the spontaneous activity preceding the stimulus onset and the EP recorded upon acoustical stimulation. These recordings, which we call EEG-EPograms, are measured simultaneously from different subdural structures, such as the auditory cortex, medial geniculate nucleus, inferior colliculus, reticular formation and the hippocampus of the cat brain during the slow wave sleep stage. Using a combined analysis procedure (C.A.P.), the relevant frequency components of spontaneous EEG and EPs, recorded simultaneously from these brain nuclei, are analyzed according to the consistent selectivity bands depicted by the determined amplitude-frequency characteristics for the SWS-stage. In parallel with the results which we obtained for the waking stage, these analyses provide also the following information: (1) there is an important congruency in the time courses of simultaneous response components in common frequency bands, especially in the alpha and beta frequency ranges; (2) there exist significant coupling and synchrony between the evoked amplitude enhancements in the simultaneously recorded single response components; (3) the inter-nuclei coherency in the brain's electrical activity is enormously increased upon stimulation;(4) the evoked response magnitude can be predicted, with reasonable accuracy, from the spontaneous activity preceding the stimulus. All these findings are discussed with reference to those obtained for the waking stage.", "contents": "Combined dynamics of EEG and evoked potentials. II. Studies of simultaneously recorded EEG-EPograms in the auditory pathway, reticular formation, and hippocampus of the cat brain during sleep. This study is carried out on single (not averaged) recordings combining the spontaneous activity preceding the stimulus onset and the EP recorded upon acoustical stimulation. These recordings, which we call EEG-EPograms, are measured simultaneously from different subdural structures, such as the auditory cortex, medial geniculate nucleus, inferior colliculus, reticular formation and the hippocampus of the cat brain during the slow wave sleep stage. Using a combined analysis procedure (C.A.P.), the relevant frequency components of spontaneous EEG and EPs, recorded simultaneously from these brain nuclei, are analyzed according to the consistent selectivity bands depicted by the determined amplitude-frequency characteristics for the SWS-stage. In parallel with the results which we obtained for the waking stage, these analyses provide also the following information: (1) there is an important congruency in the time courses of simultaneous response components in common frequency bands, especially in the alpha and beta frequency ranges; (2) there exist significant coupling and synchrony between the evoked amplitude enhancements in the simultaneously recorded single response components; (3) the inter-nuclei coherency in the brain's electrical activity is enormously increased upon stimulation;(4) the evoked response magnitude can be predicted, with reasonable accuracy, from the spontaneous activity preceding the stimulus. All these findings are discussed with reference to those obtained for the waking stage."}
{"id": "PMID:486591", "title": "Fourier analysis of spike train data.", "content": "A formal representation of nerve spike trains in the form of a sum of rectangular functions is proposed. This formal instantaneous frequency function can be Fourier analyzed. The resulting algorithm has the useful properties of spike by spike calculations and an insensitivity to the mean (carrier) spike rate. The technique is also useful for producing a smooth (filtered) reconstruction of a spike train.", "contents": "Fourier analysis of spike train data. A formal representation of nerve spike trains in the form of a sum of rectangular functions is proposed. This formal instantaneous frequency function can be Fourier analyzed. The resulting algorithm has the useful properties of spike by spike calculations and an insensitivity to the mean (carrier) spike rate. The technique is also useful for producing a smooth (filtered) reconstruction of a spike train."}
{"id": "PMID:486592", "title": "Mathematical model for the self-organization of neural networks.", "content": "Mutual inhibition between neurons combined with a learning principle similar to that proposed by Hebb is shown to secure a powerful self-organizing property for neural networks. Numerical analysis reveals that the system investigated always organizes itself into the same final state from any arbitrarily chosen initial state.", "contents": "Mathematical model for the self-organization of neural networks. Mutual inhibition between neurons combined with a learning principle similar to that proposed by Hebb is shown to secure a powerful self-organizing property for neural networks. Numerical analysis reveals that the system investigated always organizes itself into the same final state from any arbitrarily chosen initial state."}
{"id": "PMID:486593", "title": "A mathematical theory of visual hallucination patterns.", "content": "Neuronal activity in a two-dimensional net is analyzed in the neighborhood of an instability. Bifurcation theory and group theory are used to demonstrate the existence of a variety of doubly-periodic patterns, hexagons, rolls, etc., as solutions to the field equations for the net activity. It is suggested that these simple geometric patterns are the cortical concomitants of the \"form constants\" seen during visual hallucinosis.", "contents": "A mathematical theory of visual hallucination patterns. Neuronal activity in a two-dimensional net is analyzed in the neighborhood of an instability. Bifurcation theory and group theory are used to demonstrate the existence of a variety of doubly-periodic patterns, hexagons, rolls, etc., as solutions to the field equations for the net activity. It is suggested that these simple geometric patterns are the cortical concomitants of the \"form constants\" seen during visual hallucinosis."}
{"id": "PMID:486594", "title": "Activity analysis of neural networks.", "content": "In a series of articles (Leung et al., 1973, 1974; Og\u016dzt\u00f6reli, 1972, 1975, 1978, 1979; Stein et al., 1974) we have investigated some of the physiologically significant properties of a general neural model. In these papers the nature of the oscillations occuring in the model has been briefly analyzed by omitting the effects of the discrete time-lags in the interaction of neurons, although these time-lags were incoporated in the general model. In the present work we investigate the effects of the time-lags on the oscillations which are intrinsic to the neural model, depending on the structural parameters such as external inputs, interaction coefficients, self-inhibition, self-excitation and self-adaptation coefficients. The numerical solution of the neural model, the computation of the steady-state solutions and the natural modes of the oscillations around the steady-state solutions are described.", "contents": "Activity analysis of neural networks. In a series of articles (Leung et al., 1973, 1974; Og\u016dzt\u00f6reli, 1972, 1975, 1978, 1979; Stein et al., 1974) we have investigated some of the physiologically significant properties of a general neural model. In these papers the nature of the oscillations occuring in the model has been briefly analyzed by omitting the effects of the discrete time-lags in the interaction of neurons, although these time-lags were incoporated in the general model. In the present work we investigate the effects of the time-lags on the oscillations which are intrinsic to the neural model, depending on the structural parameters such as external inputs, interaction coefficients, self-inhibition, self-excitation and self-adaptation coefficients. The numerical solution of the neural model, the computation of the steady-state solutions and the natural modes of the oscillations around the steady-state solutions are described."}
{"id": "PMID:486595", "title": "A unified model for the combined temporal and spatial Broca-Sulzer effect.", "content": "A briefly pulsed light is brighter than a pulse of longer duration. For a brief flash of fixed duration and small area, enlarging a target results initially in an increase in brightness. Beyond some critical area, the target dims with further increases in size. These two phenomena are the temporal and the spatial Broca-Sulzer effect respectively. Each effect can be modeled using a generalization of the Hartline-Ratliff equation. The resulting analysis produces a mathematically unified treatment of both effects.", "contents": "A unified model for the combined temporal and spatial Broca-Sulzer effect. A briefly pulsed light is brighter than a pulse of longer duration. For a brief flash of fixed duration and small area, enlarging a target results initially in an increase in brightness. Beyond some critical area, the target dims with further increases in size. These two phenomena are the temporal and the spatial Broca-Sulzer effect respectively. Each effect can be modeled using a generalization of the Hartline-Ratliff equation. The resulting analysis produces a mathematically unified treatment of both effects."}
{"id": "PMID:486600", "title": "Fetal to maternal transfer of palmitic and linoleic acids across the sheep placenta.", "content": "Maternal and fetal blood samples were obtained at 2.5, 10 and 20 min after the injection of 3H-palmitic acid and 14C-linoleic acid into the fetal circulation of pregnant ewes. 3H-palmitic acid was easily detected in the maternal circulation within 2.5 min after its injection into the fetus resulting in an appreciable accumulation in the maternal plasma by the 20th min after injection. In contrast the presence of 14C-linoleic acid in the maternal circulation was barely detectable over the 20-min sampling period. The concentrations of total lipid and unesterified fatty acids within the fetal plasma were considerably lower than in the maternal plasma thereby providing an adverse concentration gradient for the passage of fatty acids between fetus and mother. The differential permeability of the placenta to the passage of the 3H-palmitic acid and 14C-linoleic acid is discussed in relation to their passage from fetus to mother in non-ruminant species and the adverse essential fatty acid status known to exist in the developing ruminant fetus.", "contents": "Fetal to maternal transfer of palmitic and linoleic acids across the sheep placenta. Maternal and fetal blood samples were obtained at 2.5, 10 and 20 min after the injection of 3H-palmitic acid and 14C-linoleic acid into the fetal circulation of pregnant ewes. 3H-palmitic acid was easily detected in the maternal circulation within 2.5 min after its injection into the fetus resulting in an appreciable accumulation in the maternal plasma by the 20th min after injection. In contrast the presence of 14C-linoleic acid in the maternal circulation was barely detectable over the 20-min sampling period. The concentrations of total lipid and unesterified fatty acids within the fetal plasma were considerably lower than in the maternal plasma thereby providing an adverse concentration gradient for the passage of fatty acids between fetus and mother. The differential permeability of the placenta to the passage of the 3H-palmitic acid and 14C-linoleic acid is discussed in relation to their passage from fetus to mother in non-ruminant species and the adverse essential fatty acid status known to exist in the developing ruminant fetus."}
{"id": "PMID:486601", "title": "Phototherapy and neonatal liver function.", "content": "Two groups of 'healthy' full-term infants with hyperbilirubinemia exposed to phototherapy for 72 h demonstrated no significant change in serum glutamic pyruvic transaminase (SGPT), isocitric dehydrogenase (SICD), alkaline phosphatase (SAP), heat stable alkaline phosphatase (HSAP), total protein and albumin values; these values were similar to those of a comparable group of control infants without hyperbilirubinemia. The bilirubin levels, however, decreased significantly during this period. In a separate group of full-term infants with hyperbilirubinemia, the bromsulphalein (BSP) test before and after 72 h of phototherapy also demonstrated no signficant alteration; the results were comparable to a control group of infants. Phototherapy, even for a duration of 72 h apparently does not seem to affect liver function in infants with hyperbilirubinemia.", "contents": "Phototherapy and neonatal liver function. Two groups of 'healthy' full-term infants with hyperbilirubinemia exposed to phototherapy for 72 h demonstrated no significant change in serum glutamic pyruvic transaminase (SGPT), isocitric dehydrogenase (SICD), alkaline phosphatase (SAP), heat stable alkaline phosphatase (HSAP), total protein and albumin values; these values were similar to those of a comparable group of control infants without hyperbilirubinemia. The bilirubin levels, however, decreased significantly during this period. In a separate group of full-term infants with hyperbilirubinemia, the bromsulphalein (BSP) test before and after 72 h of phototherapy also demonstrated no signficant alteration; the results were comparable to a control group of infants. Phototherapy, even for a duration of 72 h apparently does not seem to affect liver function in infants with hyperbilirubinemia."}
{"id": "PMID:486602", "title": "Hemopoietic events in human embryonic spleens at early gestational stages.", "content": "The appearance of hemopoietic progenitor cells in human embryonic spleens at early gestational stages was observed with a transmission electron microscope. In spleens of 6- to 7-week-old embryos hemocytoblasts, proerythroblasts and megakarycotyic precursors were identified, located between the mesenchymal cells, suggesting that hemopoiesis in the embryonic spleen is an extravascular process. The relationship between hemopoietic and mesenchymal cells in the embryonic spleen is discussed.", "contents": "Hemopoietic events in human embryonic spleens at early gestational stages. The appearance of hemopoietic progenitor cells in human embryonic spleens at early gestational stages was observed with a transmission electron microscope. In spleens of 6- to 7-week-old embryos hemocytoblasts, proerythroblasts and megakarycotyic precursors were identified, located between the mesenchymal cells, suggesting that hemopoiesis in the embryonic spleen is an extravascular process. The relationship between hemopoietic and mesenchymal cells in the embryonic spleen is discussed."}
{"id": "PMID:486603", "title": "Phototherapy: the cause of increased enterohepatic circulation of bilirubin in newborn infants?", "content": "The unconjugated/conjugated bilirubin ratio which was examined in the feces of 17 newborn infants submitted to phototherapy, was approximately 1:1 before, during and after the treatment. These results are not in keeping with those of Lund and Jacobsen who demonstrated an increased concentration of unconjugated bilirubin in duodenal bile. From this discrepancy one can deduce the unfavorable hypothesis that phototherapy stimulates the intestinal reabsorption of unconjugated bilirubin to the circulation.", "contents": "Phototherapy: the cause of increased enterohepatic circulation of bilirubin in newborn infants? The unconjugated/conjugated bilirubin ratio which was examined in the feces of 17 newborn infants submitted to phototherapy, was approximately 1:1 before, during and after the treatment. These results are not in keeping with those of Lund and Jacobsen who demonstrated an increased concentration of unconjugated bilirubin in duodenal bile. From this discrepancy one can deduce the unfavorable hypothesis that phototherapy stimulates the intestinal reabsorption of unconjugated bilirubin to the circulation."}
{"id": "PMID:486604", "title": "Absorption of orally administered bovine prolactin by neonatal rats.", "content": "Neonatal rats received by esophageal administration 400 ng bovine prolactin (bPRL) in 0.5 ml of diluent. Blood serum was collected by decapitation at intervals of 15, 30, 45 and 90 min after administration. Pooled samples of rat sera were assayed for the presence of immuno-reactive bPRL using radioimmunoassay procedures validated for that purpose. Significant quantities of bPRL were detected in sera from 7- and 8-day-old rats at 30 and 45 min after oral administration. However, there was no bPRL detected in sera from 7-day-old rats at 15 or 90 min after administration. There was also an absence of detectable bPRL at 45 min after administration in rats between 9 and 14 days of age. Rats older than 8 days apparently lose the ability to absorb oral bPRL or become more able to degrade the hormone.", "contents": "Absorption of orally administered bovine prolactin by neonatal rats. Neonatal rats received by esophageal administration 400 ng bovine prolactin (bPRL) in 0.5 ml of diluent. Blood serum was collected by decapitation at intervals of 15, 30, 45 and 90 min after administration. Pooled samples of rat sera were assayed for the presence of immuno-reactive bPRL using radioimmunoassay procedures validated for that purpose. Significant quantities of bPRL were detected in sera from 7- and 8-day-old rats at 30 and 45 min after oral administration. However, there was no bPRL detected in sera from 7-day-old rats at 15 or 90 min after administration. There was also an absence of detectable bPRL at 45 min after administration in rats between 9 and 14 days of age. Rats older than 8 days apparently lose the ability to absorb oral bPRL or become more able to degrade the hormone."}
{"id": "PMID:486605", "title": "Fetal rat adrenal growth and steroidogenesis in vitro after maternal dexamethasone treatment.", "content": "Two groups of female rats were treated with dexamethasone phosphate (DEX) for 24 h. One group received DEX in drinking water (10 micrograms/ml) on the 15th day of pregnancy (DEX15--16), and the second group on the 17th day of pregnancy (DEX 17--18).  The mothers and fetuses were sacrificed on the 20th and 22nd days of pregnancy. DEX reduced adrenal weights and concentration of corticosterone in plasma of the treated females and their fetuses. Fetal body weight was also reduced. However, the lightest adrenal glands were found in fetuses of the DEX17--18 mothers. Furthermore, the 19-day-old fetuses belonging to the DEX17--18 mothers had smallest amounts of corticosterone in plasma, and in plasma, and their adrenal glands produced the lowest amounts of radioactive corticosterone, 11-doxycorticosterone and 18-hydroxy-11-deoxycorticosterone from 4-14C progesterone after 1 h or incubation in vitro. In the 21-day-old fetuses belonging to both the groups of treated mothers, the production of steroids by the adrenal glands was similar and to the same extent reduced in comparison to the control fetuses.", "contents": "Fetal rat adrenal growth and steroidogenesis in vitro after maternal dexamethasone treatment. Two groups of female rats were treated with dexamethasone phosphate (DEX) for 24 h. One group received DEX in drinking water (10 micrograms/ml) on the 15th day of pregnancy (DEX15--16), and the second group on the 17th day of pregnancy (DEX 17--18).  The mothers and fetuses were sacrificed on the 20th and 22nd days of pregnancy. DEX reduced adrenal weights and concentration of corticosterone in plasma of the treated females and their fetuses. Fetal body weight was also reduced. However, the lightest adrenal glands were found in fetuses of the DEX17--18 mothers. Furthermore, the 19-day-old fetuses belonging to the DEX17--18 mothers had smallest amounts of corticosterone in plasma, and in plasma, and their adrenal glands produced the lowest amounts of radioactive corticosterone, 11-doxycorticosterone and 18-hydroxy-11-deoxycorticosterone from 4-14C progesterone after 1 h or incubation in vitro. In the 21-day-old fetuses belonging to both the groups of treated mothers, the production of steroids by the adrenal glands was similar and to the same extent reduced in comparison to the control fetuses."}
{"id": "PMID:486606", "title": "Teratogenic interaction of hyperthermia and vitamin A.", "content": "Exposure of pregnant hamsters to 60--75 min of hyperthermia on the 8th day of gestation causes malformations in some of the fetuses recovered near term. The feeding of large doses of vitamin A to pregnant hamsters on the 8th day of gestation causes many of the same types of malformations. When pregnant hamsters are treated with the minimal teratogenic hyperthermic stress plus the minimal teratogenic dose of vitamin A there is a clear augmentation of the teratogenic effect on the embryo. The implication that maternal hyperthermia may be an important synergistic factor for a variety of potentially teratogenic influences during pregnancy is discussed.", "contents": "Teratogenic interaction of hyperthermia and vitamin A. Exposure of pregnant hamsters to 60--75 min of hyperthermia on the 8th day of gestation causes malformations in some of the fetuses recovered near term. The feeding of large doses of vitamin A to pregnant hamsters on the 8th day of gestation causes many of the same types of malformations. When pregnant hamsters are treated with the minimal teratogenic hyperthermic stress plus the minimal teratogenic dose of vitamin A there is a clear augmentation of the teratogenic effect on the embryo. The implication that maternal hyperthermia may be an important synergistic factor for a variety of potentially teratogenic influences during pregnancy is discussed."}
{"id": "PMID:486607", "title": "Superoxide dismutase activity in the maternal and cord blood.", "content": "Superoxide dismutase (SOD) activity was measured in the maternal and cord blood by the modified method of Beauchamp and Fridovich, using a carbonate-buffered (pH 10.2) xanthine-xanthine oxidase system. No great differences between maternal and cord blood in erythrocyte SOD levels were observed, with the exception of whole blood; namely, washed RBC showed a SOD activity of a fairly high level, which was comparable to the activities of crude SOD, but showed no difference between them. In contrast, the SOD activity in the maternal whole blood was significantly lower than that in the cord blood. In measuring SOD activity, the serum factor has a great effect, and serum contains a substance that inhibits NBT reduction. Only one band of SOD has been detected which shows identical Rf values both in maternal and cord blood by polyacrylamide-gel electrophoresis.", "contents": "Superoxide dismutase activity in the maternal and cord blood. Superoxide dismutase (SOD) activity was measured in the maternal and cord blood by the modified method of Beauchamp and Fridovich, using a carbonate-buffered (pH 10.2) xanthine-xanthine oxidase system. No great differences between maternal and cord blood in erythrocyte SOD levels were observed, with the exception of whole blood; namely, washed RBC showed a SOD activity of a fairly high level, which was comparable to the activities of crude SOD, but showed no difference between them. In contrast, the SOD activity in the maternal whole blood was significantly lower than that in the cord blood. In measuring SOD activity, the serum factor has a great effect, and serum contains a substance that inhibits NBT reduction. Only one band of SOD has been detected which shows identical Rf values both in maternal and cord blood by polyacrylamide-gel electrophoresis."}
{"id": "PMID:486608", "title": "Heterogeneity of fetal hemoglobin in severe alpha-thalassemia.", "content": "The heterogeneity of residue 136 of the gamma-chain of human hemoglobin has been determined for a patient afflicted with severe alpha-thalassemia. Separation of the cord blood sample into the various constituent hemoglobins A, F, FI and Bart's were done on a column packed with DEAE Sephadex. The amount of glycine or alanine at position 136 was determined for hemoglobins F, FI and Bart's. The ratios determined for all three hemoglobins indicated that the G gamma/A gamma ratio is the same for all three fractions and is similar to that observed in normal cord blood samples.", "contents": "Heterogeneity of fetal hemoglobin in severe alpha-thalassemia. The heterogeneity of residue 136 of the gamma-chain of human hemoglobin has been determined for a patient afflicted with severe alpha-thalassemia. Separation of the cord blood sample into the various constituent hemoglobins A, F, FI and Bart's were done on a column packed with DEAE Sephadex. The amount of glycine or alanine at position 136 was determined for hemoglobins F, FI and Bart's. The ratios determined for all three hemoglobins indicated that the G gamma/A gamma ratio is the same for all three fractions and is similar to that observed in normal cord blood samples."}
{"id": "PMID:486609", "title": "'Minor hemoglobins': an alternative approach for evaluating glucose control in pregnancy.", "content": "'Minor hemoglobins' (Hb AI) were measured in 178 women during pregnancy and immediately postpartum by a thiobarbituric acid spectrophotometric method. The patients were classified according to an oral glucose tolerance test (GTT) and an assessment of risk factors for diabetes in normal (n = 75), probably normal (n = 72) and chemical diabetics (n = 26). 5 pregnant insulin-dependent diabetics were also studied. Hb AI was unchanged during the second and third trimesters of pregnancy and immediately postpartum (less than 6 days) in women with normal glucose tolerance. Moreover, no difference of Hb AI level was found when compared with a separate group of nonpregnant individuals (n = 15). Chemical diabetics had significantly higher mean third trimester and postpartum Hb AI than normals (p less than 0.001). The highest Hb AI levels during pregnancy were found in the insulin-dependent diabetics, although these were lower compared to a separate group of nonpregnant insulin-dependent diabetics (n = 10; p less than 0.05). During pregnancy, a significant correlation was found between Hb AI and the 2-hour value of the GTT (p less than 0.001). A positive correlation was also found between third trimester maternal Hb AI and infant birth weight (p less than 0.001). Hb AI may complement other parameters in evaluating maternal glucose tolerance during pregnancy.", "contents": "'Minor hemoglobins': an alternative approach for evaluating glucose control in pregnancy. 'Minor hemoglobins' (Hb AI) were measured in 178 women during pregnancy and immediately postpartum by a thiobarbituric acid spectrophotometric method. The patients were classified according to an oral glucose tolerance test (GTT) and an assessment of risk factors for diabetes in normal (n = 75), probably normal (n = 72) and chemical diabetics (n = 26). 5 pregnant insulin-dependent diabetics were also studied. Hb AI was unchanged during the second and third trimesters of pregnancy and immediately postpartum (less than 6 days) in women with normal glucose tolerance. Moreover, no difference of Hb AI level was found when compared with a separate group of nonpregnant individuals (n = 15). Chemical diabetics had significantly higher mean third trimester and postpartum Hb AI than normals (p less than 0.001). The highest Hb AI levels during pregnancy were found in the insulin-dependent diabetics, although these were lower compared to a separate group of nonpregnant insulin-dependent diabetics (n = 10; p less than 0.05). During pregnancy, a significant correlation was found between Hb AI and the 2-hour value of the GTT (p less than 0.001). A positive correlation was also found between third trimester maternal Hb AI and infant birth weight (p less than 0.001). Hb AI may complement other parameters in evaluating maternal glucose tolerance during pregnancy."}
{"id": "PMID:486610", "title": "Metabolic response to thyroxine in the newborn pig.", "content": "The oxygen consumption rate (ml O2/kg/min), the pulse rate, respiratory frequency and body temperatures were measured after a single subcutaneous injection of thyroxine (80 micrograms/kg) or adrenaline (200 micrograms/kg) in unfasted pigs aged 1--11 days. Thyroxine produced a metabolic response after a latent period and potentiated the metabolic action of adrenaline. As reported in the literature, the existence of an immediate metabolic response to thyroxine was not evidenced. The obtained results support the view that in the newborn pig the physiological rise in metabolism which leads to metabolic stability might be caused by thyroxine, whose free fraction and highest level in the blood occurs shortly after birth. Potentiation of adrenaline action by thyroxine can contribute to this process.", "contents": "Metabolic response to thyroxine in the newborn pig. The oxygen consumption rate (ml O2/kg/min), the pulse rate, respiratory frequency and body temperatures were measured after a single subcutaneous injection of thyroxine (80 micrograms/kg) or adrenaline (200 micrograms/kg) in unfasted pigs aged 1--11 days. Thyroxine produced a metabolic response after a latent period and potentiated the metabolic action of adrenaline. As reported in the literature, the existence of an immediate metabolic response to thyroxine was not evidenced. The obtained results support the view that in the newborn pig the physiological rise in metabolism which leads to metabolic stability might be caused by thyroxine, whose free fraction and highest level in the blood occurs shortly after birth. Potentiation of adrenaline action by thyroxine can contribute to this process."}
{"id": "PMID:486611", "title": "The metabolic effects of cold exposure in the newborn rabbit.", "content": "Blood glucose, lactate, plasma free fatty acid and plasma and tissue individual free amino acid levels were followed in newborn rabbits exposed for 10 h to an environmental temperature of 25 degrees C. Severe hypothermia developed with an increase of blood lactate and accumulation of total free amino acids in plasma and liver. Alanine, isoleucine, leucine, valine, phenylalanine, tyrosine, ornithine and taurine were elevated in the plasma; alanine and ornithine in the liver; leucine and isoleucine in the muscle.", "contents": "The metabolic effects of cold exposure in the newborn rabbit. Blood glucose, lactate, plasma free fatty acid and plasma and tissue individual free amino acid levels were followed in newborn rabbits exposed for 10 h to an environmental temperature of 25 degrees C. Severe hypothermia developed with an increase of blood lactate and accumulation of total free amino acids in plasma and liver. Alanine, isoleucine, leucine, valine, phenylalanine, tyrosine, ornithine and taurine were elevated in the plasma; alanine and ornithine in the liver; leucine and isoleucine in the muscle."}
{"id": "PMID:486612", "title": "Effect of hypothermia on plasma metabolites in preterm newborn infants with particular references to plasma free amino acids.", "content": "Blood glucose, blood lactate, plasma free fatty acids, plasma alpha-amino nitrogen, plasma free amino acids and blood urea nitrogen were measured in 15 hypothermic preterm infants (mean rectal temperature: 34.5 +/- 0.28 degrees C) during a 2-hour 'warming-up' period following admission to our neonatal intensive care unit. Hypothermia was associated with lactacidemia and hyperalaninemia. The increase in body temperature of the infants resulted in the decrease of lactacidemia and hyperalaninemia. Normal levels of other metabolites remained unchanged.", "contents": "Effect of hypothermia on plasma metabolites in preterm newborn infants with particular references to plasma free amino acids. Blood glucose, blood lactate, plasma free fatty acids, plasma alpha-amino nitrogen, plasma free amino acids and blood urea nitrogen were measured in 15 hypothermic preterm infants (mean rectal temperature: 34.5 +/- 0.28 degrees C) during a 2-hour 'warming-up' period following admission to our neonatal intensive care unit. Hypothermia was associated with lactacidemia and hyperalaninemia. The increase in body temperature of the infants resulted in the decrease of lactacidemia and hyperalaninemia. Normal levels of other metabolites remained unchanged."}
{"id": "PMID:486614", "title": "A longitudinal CNV study in a group of five bipolar cyclothymic patients.", "content": "The authors carried out a longitudinal study in five subjects with bipolar cyclothymic psychosis, recording contingent negative variations in the same patients in the different phases of illness and under normal clinical conditions. An average voltage decrease was found in the depressive phases and a more conspicuous decrease in the manic phases. Furthermore, an evident postimperative negative variation was present in four subjects during the manic phase. The authors set forth tentative psychological and neurophysiological interpretation of their results.", "contents": "A longitudinal CNV study in a group of five bipolar cyclothymic patients. The authors carried out a longitudinal study in five subjects with bipolar cyclothymic psychosis, recording contingent negative variations in the same patients in the different phases of illness and under normal clinical conditions. An average voltage decrease was found in the depressive phases and a more conspicuous decrease in the manic phases. Furthermore, an evident postimperative negative variation was present in four subjects during the manic phase. The authors set forth tentative psychological and neurophysiological interpretation of their results."}
{"id": "PMID:486615", "title": "Neurolepticlike actions of l-methadone: effect on mescaline-induced altered behavior and on tissue levels of mescaline in mice.", "content": "Mice were injected ip with either saline, l-methadone (2.5, 5, 20 mg/kg), perphenazine (1, 10, 15 mg/kg), or chlorprothixene (1.25, 2.5, 15 mg/kg) 30 min prior to mescaline-14C (25 mg/kg). Mescaline-induced behavioral changes such as agitation, excitement, slight increase in ventilation, and fright to sound stimuli were prevented by all doses of three drugs, and head-shaking, scratching, and locomotor-increasing effects by 5 and 20 mg/kg methadone and by all doses of both neuroleptics. Catalepticlike state and moderate to marked hypothermia induced by all doses of chlorprothixene, 10 and 15 mg/kg perphenazine, and 20 mg/kg methadone were not reversed by mescaline. Chlorprothixene (all doses), perphenazine (10, 15 mg/kg), and methadone (5, 20 mg/kg) caused marked retention of mescaline and its deaminated metabolite, 3, 4, 5-trimethoxyphenyl acetic acid in both brain and plasma. The fact that relatively higher doses of methadone than neuroleptics are needed to ensure effective antagonism to mescaline action tends to indicate a less specific interaction of the opiate with the neuroleptic/dopamine receptor proposed for central mescaline effects.", "contents": "Neurolepticlike actions of l-methadone: effect on mescaline-induced altered behavior and on tissue levels of mescaline in mice. Mice were injected ip with either saline, l-methadone (2.5, 5, 20 mg/kg), perphenazine (1, 10, 15 mg/kg), or chlorprothixene (1.25, 2.5, 15 mg/kg) 30 min prior to mescaline-14C (25 mg/kg). Mescaline-induced behavioral changes such as agitation, excitement, slight increase in ventilation, and fright to sound stimuli were prevented by all doses of three drugs, and head-shaking, scratching, and locomotor-increasing effects by 5 and 20 mg/kg methadone and by all doses of both neuroleptics. Catalepticlike state and moderate to marked hypothermia induced by all doses of chlorprothixene, 10 and 15 mg/kg perphenazine, and 20 mg/kg methadone were not reversed by mescaline. Chlorprothixene (all doses), perphenazine (10, 15 mg/kg), and methadone (5, 20 mg/kg) caused marked retention of mescaline and its deaminated metabolite, 3, 4, 5-trimethoxyphenyl acetic acid in both brain and plasma. The fact that relatively higher doses of methadone than neuroleptics are needed to ensure effective antagonism to mescaline action tends to indicate a less specific interaction of the opiate with the neuroleptic/dopamine receptor proposed for central mescaline effects."}
{"id": "PMID:486616", "title": "Hydroxylated metabolites of tricyclic antidepressants: preclinical assessment of activity.", "content": "Studies investigating a possible relationship between the plasma concentration of tricyclic antidepressants and clinical response have measured only the tertiary and secondary amine forms of these drugs. The present study shows that the hydroxy metabolites of tricyclic antidepressants might also be active. Hydroxylated imipramine, desipramine, chlorimipramine, and nortriptyline inhibit the uptake of norepinephrine and serotonin into synaptosomes to the same extent as do their parent compounds. Hydroxylated nortriptyline and imipramine reverse or prevent reserpine-induced motor retardation and ptosis. Following chronic imipramine, significant steady-state concentrations of unconjugated hydroxylated metabolites are present in rat tissues including the cerebrospinal fluid. Accounting for steady-state concentrations of hydroxylated metabolites of tricyclic antidepressants in man may help to clarify whether there is a relationship between active drug concentration and clinical effect.", "contents": "Hydroxylated metabolites of tricyclic antidepressants: preclinical assessment of activity. Studies investigating a possible relationship between the plasma concentration of tricyclic antidepressants and clinical response have measured only the tertiary and secondary amine forms of these drugs. The present study shows that the hydroxy metabolites of tricyclic antidepressants might also be active. Hydroxylated imipramine, desipramine, chlorimipramine, and nortriptyline inhibit the uptake of norepinephrine and serotonin into synaptosomes to the same extent as do their parent compounds. Hydroxylated nortriptyline and imipramine reverse or prevent reserpine-induced motor retardation and ptosis. Following chronic imipramine, significant steady-state concentrations of unconjugated hydroxylated metabolites are present in rat tissues including the cerebrospinal fluid. Accounting for steady-state concentrations of hydroxylated metabolites of tricyclic antidepressants in man may help to clarify whether there is a relationship between active drug concentration and clinical effect."}
{"id": "PMID:486617", "title": "Human leukocyte antigen system not closely linked to or associated with bipolar manic-depressive illness.", "content": "An association and linkage study of the HLA system and bipolar affective illness is reported. HLA B-14 showed an increased frequency and HLA-Bw27 a decreased frequency in 92 bipolar patients compared to 210 controls, but significance is not reached when appropriate statistical corrections are made. It is shown that ethnological differences can lead to sampling biases; a purported increased frequency of HLA-Bw 16 in Ashkenazi Jewish bipolar patients is negated when ethnologically similar controls are used. The transmission of HLA alleles in nine families with at least two generations of affective illness revealed independent assortment, and nonlinkage to either locus A or B was demonstrated using a multigenerational method of linkage analysis. The nonreplicability of the HLA association studies, and the failure to demonstrate linkage of the HLA loci with afe development of affective illness, although further analyses are necessary.", "contents": "Human leukocyte antigen system not closely linked to or associated with bipolar manic-depressive illness. An association and linkage study of the HLA system and bipolar affective illness is reported. HLA B-14 showed an increased frequency and HLA-Bw27 a decreased frequency in 92 bipolar patients compared to 210 controls, but significance is not reached when appropriate statistical corrections are made. It is shown that ethnological differences can lead to sampling biases; a purported increased frequency of HLA-Bw 16 in Ashkenazi Jewish bipolar patients is negated when ethnologically similar controls are used. The transmission of HLA alleles in nine families with at least two generations of affective illness revealed independent assortment, and nonlinkage to either locus A or B was demonstrated using a multigenerational method of linkage analysis. The nonreplicability of the HLA association studies, and the failure to demonstrate linkage of the HLA loci with afe development of affective illness, although further analyses are necessary."}
{"id": "PMID:486618", "title": "Qualitative identity of cerebral neuronal membrane actions of 5HT LSD, and CPZ.", "content": "Extra- and intracellular recording of the cerebral cortical actions of close-arterially injected serotonin (5HT) and LSD in the cat shows them to be powerful synaptic inhibitors. They are specifically and differentially blocked by chlorpromazine (CPZ). The membrane parameters including spike generation, polarization, transmembrane conductance, and IPSPs show that all three produce qualitatively identical changes, which must, therefore, be presumed to act on the same receptors with block by CPZ taking place because of competitive inhibition. The relation of these neuronal membrane findings to the characteristic actions of LSD and CPZ in mental disturbance is considered in relation to a general concept of cerebral synaptic dysfunction.", "contents": "Qualitative identity of cerebral neuronal membrane actions of 5HT LSD, and CPZ. Extra- and intracellular recording of the cerebral cortical actions of close-arterially injected serotonin (5HT) and LSD in the cat shows them to be powerful synaptic inhibitors. They are specifically and differentially blocked by chlorpromazine (CPZ). The membrane parameters including spike generation, polarization, transmembrane conductance, and IPSPs show that all three produce qualitatively identical changes, which must, therefore, be presumed to act on the same receptors with block by CPZ taking place because of competitive inhibition. The relation of these neuronal membrane findings to the characteristic actions of LSD and CPZ in mental disturbance is considered in relation to a general concept of cerebral synaptic dysfunction."}
{"id": "PMID:486619", "title": "Screening for PKU heterozygosity in bipolar affectively ill patients.", "content": "There were no significant differences noted between bipolar manic-depressive patients and normal controls for plasma phenylalanine or tyrosine following an L-phenylalanine loading test given to determine if some affective illness may be related to the heterozygous phenotypic expression of phenylketonuria (reduced liver phenylalanine hydroxylase). The test was able to distinguish known PKU heterozygotes from the other subjects. It is possible that other heterozygous states may be implicated in the development of some psychiatric disorders.", "contents": "Screening for PKU heterozygosity in bipolar affectively ill patients. There were no significant differences noted between bipolar manic-depressive patients and normal controls for plasma phenylalanine or tyrosine following an L-phenylalanine loading test given to determine if some affective illness may be related to the heterozygous phenotypic expression of phenylketonuria (reduced liver phenylalanine hydroxylase). The test was able to distinguish known PKU heterozygotes from the other subjects. It is possible that other heterozygous states may be implicated in the development of some psychiatric disorders."}
{"id": "PMID:486621", "title": "Lithium carbonate in the treatment of schizophrenia and schizo-affective disorder: review and hypothesis.", "content": "The success of lithium carbonate in the treatment of manic-depressive illness has led to its use in many other psychiatric disorders. Within this group, schizophrenia and schizo-affective disorder have come under the closest scrutiny. The literature, however, is difficult to reconcile. Conclusions are often contradictory, methodologies confusing, and well-designed studies few in number. In this paper, the authors have attempted to extract common denominators that may prove of value in delineating those symptoms which predict therapeutic response to lithium carbonate.", "contents": "Lithium carbonate in the treatment of schizophrenia and schizo-affective disorder: review and hypothesis. The success of lithium carbonate in the treatment of manic-depressive illness has led to its use in many other psychiatric disorders. Within this group, schizophrenia and schizo-affective disorder have come under the closest scrutiny. The literature, however, is difficult to reconcile. Conclusions are often contradictory, methodologies confusing, and well-designed studies few in number. In this paper, the authors have attempted to extract common denominators that may prove of value in delineating those symptoms which predict therapeutic response to lithium carbonate."}
{"id": "PMID:486623", "title": "Heart rate changes with exercise and voluntary heart rate acceleration.", "content": "The present study examined the relationship between heart rate (HR) changes accompanying isometric and isotonic exercises and HR changes during attempted voluntary HR acceleration. Substantial cardiac accelerations accompanied both types of exertion, with the isotonic exercise attracting the larger magnitude HR changes. Significant HR increases were also observed during attempted voluntary HR acceleration both with and without feedback; however, feedback prompted larger HR increases than instructions alone. The HR changes accompanying both types of exercise reliably predicted the extent of voluntary HR increase but only for the condition in which feedback was available. This occurred in spite of the absence of observable EMG changes during attempted HR acceleration. Changes in respiration rate during voluntary HR increase were highly correlated with changes in HR. A similar co-variation occurred with the isotonic exercise but was absent with the isometric exercise. The results are discussed in terms of the possible mediational mechanisms underlying voluntary HR acceleration.", "contents": "Heart rate changes with exercise and voluntary heart rate acceleration. The present study examined the relationship between heart rate (HR) changes accompanying isometric and isotonic exercises and HR changes during attempted voluntary HR acceleration. Substantial cardiac accelerations accompanied both types of exertion, with the isotonic exercise attracting the larger magnitude HR changes. Significant HR increases were also observed during attempted voluntary HR acceleration both with and without feedback; however, feedback prompted larger HR increases than instructions alone. The HR changes accompanying both types of exercise reliably predicted the extent of voluntary HR increase but only for the condition in which feedback was available. This occurred in spite of the absence of observable EMG changes during attempted HR acceleration. Changes in respiration rate during voluntary HR increase were highly correlated with changes in HR. A similar co-variation occurred with the isotonic exercise but was absent with the isometric exercise. The results are discussed in terms of the possible mediational mechanisms underlying voluntary HR acceleration."}
{"id": "PMID:486624", "title": "Heart-rate correlates of childhood activities: play, exploration, problem-solving and day-dreaming.", "content": "Heart-rate was monitored while children were engaged in different activities. The first study examined the hypothesis that the behavioural distinction between exploration and play would be supported by the psychophysiological measure. Two-year-olds showed suppression of heart rate variability (HRV) during exploration, and even more during solution of a puzzle, suggesting that the task demands these two activities were greater than those during play. The second study demonstrated a relationship between HRV during daydreaming and during problem solving in 9-year-old children which was similar to that obtained between play and problem solving in younger children. The correlative use of HRV thus strengthened functional interpretations of behaviour.", "contents": "Heart-rate correlates of childhood activities: play, exploration, problem-solving and day-dreaming. Heart-rate was monitored while children were engaged in different activities. The first study examined the hypothesis that the behavioural distinction between exploration and play would be supported by the psychophysiological measure. Two-year-olds showed suppression of heart rate variability (HRV) during exploration, and even more during solution of a puzzle, suggesting that the task demands these two activities were greater than those during play. The second study demonstrated a relationship between HRV during daydreaming and during problem solving in 9-year-old children which was similar to that obtained between play and problem solving in younger children. The correlative use of HRV thus strengthened functional interpretations of behaviour."}
{"id": "PMID:486625", "title": "Differential effects of state and trait anxiety on heart rate responses and reaction time.", "content": "College students were tested on 10 trials of a reaction time task, using a variable interval warning signal, while heart rate was recorded. Subjects were divided into high and low anxiety groups using a median split based on their scores on a modification of the trait scale of the State-Trait Anxiety Inventory. The low anxiety group exhibited faster reaction times, shorter latency heart rate deceleration following warning signal onset, and a more consistent deceleration immediately prior to the respond signal than the high anxiety group. When the same subjects were divided into groups according to state anxiety, no group differences were found in either heart rate or reaction time. In Part II of the study, subjects listened to six 15 s presentations of a 70 dbA, 250 Hz tone. No group differences in heart rate responses to the tones were found for either state or trait anxiety groups.", "contents": "Differential effects of state and trait anxiety on heart rate responses and reaction time. College students were tested on 10 trials of a reaction time task, using a variable interval warning signal, while heart rate was recorded. Subjects were divided into high and low anxiety groups using a median split based on their scores on a modification of the trait scale of the State-Trait Anxiety Inventory. The low anxiety group exhibited faster reaction times, shorter latency heart rate deceleration following warning signal onset, and a more consistent deceleration immediately prior to the respond signal than the high anxiety group. When the same subjects were divided into groups according to state anxiety, no group differences were found in either heart rate or reaction time. In Part II of the study, subjects listened to six 15 s presentations of a 70 dbA, 250 Hz tone. No group differences in heart rate responses to the tones were found for either state or trait anxiety groups."}
{"id": "PMID:486626", "title": "Successful and unsuccessful university students: quantitative hemispheric EEG differences.", "content": "Quantitative measurements of EEG amplitudes were obtained on academically successful and unsuccessful university students during a 5 min, eyes closed, relaxed state. Integrations measured continuously the fully rectified amplitudes of brain waves cumulated over successive epochs of 5 s, regardless of the correlation of a wave to a particular frequency band. The poor academic students, as a group, displayed a significantly higher right/left variance ratio of the cumulated amplitudes at both the temporal and occipital sites than the academically successful group. Upon examining the histograms for these cumulated amplitudes, it was found that the unsuccessful students displayed a greater number of polymodal (non-Gaussian) distributions, particularly in the right hemisphere. These data, which resemble patterns often found in depression, suggest a relative disorganization of right hemispheric activity in the unsuccessful students, and within limits, seems related to the cognitive and/or emotional stability of these students.", "contents": "Successful and unsuccessful university students: quantitative hemispheric EEG differences. Quantitative measurements of EEG amplitudes were obtained on academically successful and unsuccessful university students during a 5 min, eyes closed, relaxed state. Integrations measured continuously the fully rectified amplitudes of brain waves cumulated over successive epochs of 5 s, regardless of the correlation of a wave to a particular frequency band. The poor academic students, as a group, displayed a significantly higher right/left variance ratio of the cumulated amplitudes at both the temporal and occipital sites than the academically successful group. Upon examining the histograms for these cumulated amplitudes, it was found that the unsuccessful students displayed a greater number of polymodal (non-Gaussian) distributions, particularly in the right hemisphere. These data, which resemble patterns often found in depression, suggest a relative disorganization of right hemispheric activity in the unsuccessful students, and within limits, seems related to the cognitive and/or emotional stability of these students."}
{"id": "PMID:486627", "title": "Personality and male-female influences on the EEG alpha rhythm.", "content": "The amplitude and frequency of the alpha rhythm was measured in electroencephalic recordings from 45 male and 46 female university students. The mean alpha frequency of the female group was significantly greater than the male group. There was no significant difference in alpha frequency between females in the first half of the menstrual cycle and those in the second half. The relationship between questionnaire measures of psychoticism, extraversion and neuroticism, and the alpha rhythm was investigated using analysis of variance, correlation analysis and factor analysis. Alpha amplitude was significantly greater in extraverted subjects, alpha amplitude and extraversion scores were significantly correlated and both had high loadings on a single factor. No relationship between psychoticism or neuroticism and alpha frequency or amplitude was observed.", "contents": "Personality and male-female influences on the EEG alpha rhythm. The amplitude and frequency of the alpha rhythm was measured in electroencephalic recordings from 45 male and 46 female university students. The mean alpha frequency of the female group was significantly greater than the male group. There was no significant difference in alpha frequency between females in the first half of the menstrual cycle and those in the second half. The relationship between questionnaire measures of psychoticism, extraversion and neuroticism, and the alpha rhythm was investigated using analysis of variance, correlation analysis and factor analysis. Alpha amplitude was significantly greater in extraverted subjects, alpha amplitude and extraversion scores were significantly correlated and both had high loadings on a single factor. No relationship between psychoticism or neuroticism and alpha frequency or amplitude was observed."}
{"id": "PMID:486628", "title": "The effect of placebo and tricyclic antidepressants on cortical evoked potentials in depressed patients.", "content": "Cortical evoked potentials in depressives were recorded in order to study stimulus intensity control in this group, and also to study the effect of treatment. In a sample of 33 psychiatric patients, the amplitudes of visual and auditory evoked potentials were found to be significantly greater during depression, than at recovery. The changes which appeared to be due to recovery were found not only in patients treated with tricyclic antidepressants (n = 22), but in those who had recovered following treatment with placebo (n = 11). The changes were most evident in the auditory system. In addition, no diagnostic predictors differentiated depressed patients who subsequently recovered on placebo from those who did not. It is concluded that: (i) cortical evoked potentials may change in amplitude with emotional state; (ii) placebo may have physiological consequences usually attributed to drugs alone.", "contents": "The effect of placebo and tricyclic antidepressants on cortical evoked potentials in depressed patients. Cortical evoked potentials in depressives were recorded in order to study stimulus intensity control in this group, and also to study the effect of treatment. In a sample of 33 psychiatric patients, the amplitudes of visual and auditory evoked potentials were found to be significantly greater during depression, than at recovery. The changes which appeared to be due to recovery were found not only in patients treated with tricyclic antidepressants (n = 22), but in those who had recovered following treatment with placebo (n = 11). The changes were most evident in the auditory system. In addition, no diagnostic predictors differentiated depressed patients who subsequently recovered on placebo from those who did not. It is concluded that: (i) cortical evoked potentials may change in amplitude with emotional state; (ii) placebo may have physiological consequences usually attributed to drugs alone."}
{"id": "PMID:486629", "title": "The orienting response and stimulus significance: some comments.", "content": "Bernstein (1969, 1979) has argued that Skolov's (1963) model is not sufficient to account for the data concerning orienting response (OR) elicitation to stimulus change. He has argued that following the registration of stimulus mismatch, an additional stage of stimulus evaluation occurs and that only stimuli which represent change and are judged as 'significant' elicit ORs. The present paper takes issue with this assertion. It is argued that the data on which the 'significance hypothesis' was originally based are not replicable and that the argument, as it was formulated originally, is tautological. It is also argued that individual differences in OR responsiveness are more parsimoniously explained in terms of individual response stereotypy and that differential responsiveness to different sorts of stimulus change poses problems for the significance hypothesis. Evidence is also reviewed which suggests that the relationship between stimulus change and stimulus significance is additive rather than multiplicative as suggested by Bernstein, i.e., stimulus change and stimulus significance are each sufficient for OR elicitation. Finally, it is concluded that the concept of 'significance', introduced operationally, may be of value in terms of examining the role of the OR in information processing.", "contents": "The orienting response and stimulus significance: some comments. Bernstein (1969, 1979) has argued that Skolov's (1963) model is not sufficient to account for the data concerning orienting response (OR) elicitation to stimulus change. He has argued that following the registration of stimulus mismatch, an additional stage of stimulus evaluation occurs and that only stimuli which represent change and are judged as 'significant' elicit ORs. The present paper takes issue with this assertion. It is argued that the data on which the 'significance hypothesis' was originally based are not replicable and that the argument, as it was formulated originally, is tautological. It is also argued that individual differences in OR responsiveness are more parsimoniously explained in terms of individual response stereotypy and that differential responsiveness to different sorts of stimulus change poses problems for the significance hypothesis. Evidence is also reviewed which suggests that the relationship between stimulus change and stimulus significance is additive rather than multiplicative as suggested by Bernstein, i.e., stimulus change and stimulus significance are each sufficient for OR elicitation. Finally, it is concluded that the concept of 'significance', introduced operationally, may be of value in terms of examining the role of the OR in information processing."}
{"id": "PMID:486630", "title": "Synchronization of respiration.", "content": "In order to study synchronization of respiration, three different videofragments were presented to 21 normal subjects. Each fragment showed a 'therapeutic interview' specially performed for this purpose, with a 'patient' breathing in a particular way. The respiration of model 1 was deep, slow and clearly audible, the pattern of model 2 was rapid, superficial and slightly audible and that of model 3 was normal and hardly perceptible. The fragments were presented in three different sequences and each sequence was followed by seven subjects. Respiration amplitude (Vt), respiration irregularity (SD of Vt), respiration frequency (RF) and frequency of skin conductance responses (SCRF) were measured. Changes in mean values of these variables were demonstrated during the fragments; an increase in RF was the most pronounced and reliable change. These 'overall' physiological changes did not support the synchronization hypothesis, nor were they at variance with it: they seemed to be effects of attention. A detailed (cycle by cycle) analysis did reveal a synchronization effect in fragment 1. The number of cycles in the subject which were in rhythm with the model's respiration was significantly larger than could have been expected by chance. The effect of synchronization, however, was small and transient as it did not affect the mean RF and Vt of fragment 1.", "contents": "Synchronization of respiration. In order to study synchronization of respiration, three different videofragments were presented to 21 normal subjects. Each fragment showed a 'therapeutic interview' specially performed for this purpose, with a 'patient' breathing in a particular way. The respiration of model 1 was deep, slow and clearly audible, the pattern of model 2 was rapid, superficial and slightly audible and that of model 3 was normal and hardly perceptible. The fragments were presented in three different sequences and each sequence was followed by seven subjects. Respiration amplitude (Vt), respiration irregularity (SD of Vt), respiration frequency (RF) and frequency of skin conductance responses (SCRF) were measured. Changes in mean values of these variables were demonstrated during the fragments; an increase in RF was the most pronounced and reliable change. These 'overall' physiological changes did not support the synchronization hypothesis, nor were they at variance with it: they seemed to be effects of attention. A detailed (cycle by cycle) analysis did reveal a synchronization effect in fragment 1. The number of cycles in the subject which were in rhythm with the model's respiration was significantly larger than could have been expected by chance. The effect of synchronization, however, was small and transient as it did not affect the mean RF and Vt of fragment 1."}
{"id": "PMID:486681", "title": "The estimation of dental caries incidence in the presence of diagnostic error.", "content": "Diagnostic error in large scale screening programs for dental caries is frequent, as when teeth initially classified as carious are later diagnosed as never having been affected by caries. This paper presents a general formulation of diagnostic error in dental caries screening. The basic parameter of the formulation is the caries incidence rate. The general formulation of this paper permits an explicit comparison, in a common notation, of two specific models of diagnostic error--one due to Carlos and Senning, the other to Lu. Each model gives rise to a consistent estimator of the incidence rate. The distributional properties of these estimators had not previously been examined because of the dependence of teeth in the same mouth with respect to caries experience. Using the present formulation, the sampling scheme may be regarded as a one-stage cluster sample with mouths as clusters. This approach accounts for intracluster dependence thus permitting the derivation of an estimator of the relevant covariance matrix and a confidence interval for the incidence rate.", "contents": "The estimation of dental caries incidence in the presence of diagnostic error. Diagnostic error in large scale screening programs for dental caries is frequent, as when teeth initially classified as carious are later diagnosed as never having been affected by caries. This paper presents a general formulation of diagnostic error in dental caries screening. The basic parameter of the formulation is the caries incidence rate. The general formulation of this paper permits an explicit comparison, in a common notation, of two specific models of diagnostic error--one due to Carlos and Senning, the other to Lu. Each model gives rise to a consistent estimator of the incidence rate. The distributional properties of these estimators had not previously been examined because of the dependence of teeth in the same mouth with respect to caries experience. Using the present formulation, the sampling scheme may be regarded as a one-stage cluster sample with mouths as clusters. This approach accounts for intracluster dependence thus permitting the derivation of an estimator of the relevant covariance matrix and a confidence interval for the incidence rate."}
{"id": "PMID:486682", "title": "Detection of a treatment effect when not all experimental subjects will respond to treatment.", "content": "Non-responders attenuate average response an inflate sample variance, reducing the power of standard parametric tests. A new Fisher's type randomization test, which has no parametric analogue, is recommended when not all subjects may be capable of responding to treatment. The new test was evaluated by Monte Carlo means and applied to drug abuse data and to virus titre data. In most trial applications the new test proved to be more sensitive to treatment effects than Student's t.", "contents": "Detection of a treatment effect when not all experimental subjects will respond to treatment. Non-responders attenuate average response an inflate sample variance, reducing the power of standard parametric tests. A new Fisher's type randomization test, which has no parametric analogue, is recommended when not all subjects may be capable of responding to treatment. The new test was evaluated by Monte Carlo means and applied to drug abuse data and to virus titre data. In most trial applications the new test proved to be more sensitive to treatment effects than Student's t."}
{"id": "PMID:486683", "title": "Restricted randomization designs in clinical trials.", "content": "Though therapeutic clinical trials are often categorized as using either \"randomization\" or \"historical controls\" as a basis for treatment evaluation, pure random assignment of treatments is rarely employed. Instead various restricted randomization designs are used. The restrictions include the balancing of treatment assignments over time and the stratification of the assignment with regard to covariates that may affect response. Restricted randomization designs for clinical trials differ from those of other experimental areas because patients arrive sequentially and a balanced design cannot be ensured. The major restricted randomization designs and arguments concerning the proper role of stratification are reviewed here. The effect of randomization restrictions on the validity of significance tests is discussed.", "contents": "Restricted randomization designs in clinical trials. Though therapeutic clinical trials are often categorized as using either \"randomization\" or \"historical controls\" as a basis for treatment evaluation, pure random assignment of treatments is rarely employed. Instead various restricted randomization designs are used. The restrictions include the balancing of treatment assignments over time and the stratification of the assignment with regard to covariates that may affect response. Restricted randomization designs for clinical trials differ from those of other experimental areas because patients arrive sequentially and a balanced design cannot be ensured. The major restricted randomization designs and arguments concerning the proper role of stratification are reviewed here. The effect of randomization restrictions on the validity of significance tests is discussed."}
{"id": "PMID:486686", "title": "Prophylaxis and therapy of HBsAg positive hepatitis.", "content": "Different forms of prophylaxis and therapy of HBsAg positive hepatitis are discussed. Prevention of HBsAg positive hepatitis has been attempted by passive and active immunisation. For passive immunisation against parenteral infections hepatitis B immunoglobulin (HBIG) of high titer has to be used. However, the protection provided by HBIG is incomplete and temporary. Therefore active immunisation is to be preferred for protection of high risk groups. Experiments using the 22 nm spheric particles prepared from plasma of chronic carriers showed the efficacy of this type of vaccine. The direct way to treat the different forms of hepatitis B is the eradication of the virus from the body. Success has been claimed with the use of interferon and adenine arabinoside. However, this antiviral therapy is still in an experimental stage. In some forms of HBsAg positive hepatitis the liver damage is supposed to be the result of an immune response against the virus. Immunosuppression in these conditions failed, however, to show a beneficial effect. Corticosteroids turned out to be harmful in all forms of acute hepatitis and are therefore contraindicated. Chronic hepatitis B seems to be caused by the inability of the immune system to clear the virus. Successful results have been claimed employing immune stimulative agents like BCG, levamisole, and transfer factor. Most of these reports, however, are anecdotycal and the more comprehensive studies are uncontrolled.", "contents": "Prophylaxis and therapy of HBsAg positive hepatitis. Different forms of prophylaxis and therapy of HBsAg positive hepatitis are discussed. Prevention of HBsAg positive hepatitis has been attempted by passive and active immunisation. For passive immunisation against parenteral infections hepatitis B immunoglobulin (HBIG) of high titer has to be used. However, the protection provided by HBIG is incomplete and temporary. Therefore active immunisation is to be preferred for protection of high risk groups. Experiments using the 22 nm spheric particles prepared from plasma of chronic carriers showed the efficacy of this type of vaccine. The direct way to treat the different forms of hepatitis B is the eradication of the virus from the body. Success has been claimed with the use of interferon and adenine arabinoside. However, this antiviral therapy is still in an experimental stage. In some forms of HBsAg positive hepatitis the liver damage is supposed to be the result of an immune response against the virus. Immunosuppression in these conditions failed, however, to show a beneficial effect. Corticosteroids turned out to be harmful in all forms of acute hepatitis and are therefore contraindicated. Chronic hepatitis B seems to be caused by the inability of the immune system to clear the virus. Successful results have been claimed employing immune stimulative agents like BCG, levamisole, and transfer factor. Most of these reports, however, are anecdotycal and the more comprehensive studies are uncontrolled."}
{"id": "PMID:486687", "title": "Radioimmunoassay determination of two main cross-reacting viral antigens during radiation and Rauscher induced leukemias.", "content": "The two main proteins p 30 and gp 70 from the Rauscher leukemia virus (RLV) have been isolated, labeled with 125I and used in radioimmune competition assays to estimate the amount of cross-reacting antigens produced during the evolution of various leukemias. The Rauscher leukemia in Balb/c mice, the radiation induced leukemia (RadLV) in C57Bl mice and two types derived therefrom by serial passages in mice (RadLV-RS) and in rats (RadLV-rat) were studied. Whereas the p 30 from RLV or RadLV (rat) viruses showed complete identity, the cross-reaction of their gp 70 proteins wasonly partial. The main findings in the tissues were the following: 1. The concentration of p 30 and gp 70 antigens increased much more during the RadLV-rat leukemia than in irradiated or RadLV (RS) treated mice. 2. In the serum, the ratio p 30/gp 70 was in general higher than one in the Rauscher leukemic mice and less than one in all types of RadLV leukemias. 3. In the spleen, RLV and RadLV (RS) infected mice had higher levels of p 30 than of gp 70. The reverse occurred in irradiated mice and in leukemic rats. Finally, the catabolic degradation of labeled RLV gp 70 was similar in normal and leukemic mice.", "contents": "Radioimmunoassay determination of two main cross-reacting viral antigens during radiation and Rauscher induced leukemias. The two main proteins p 30 and gp 70 from the Rauscher leukemia virus (RLV) have been isolated, labeled with 125I and used in radioimmune competition assays to estimate the amount of cross-reacting antigens produced during the evolution of various leukemias. The Rauscher leukemia in Balb/c mice, the radiation induced leukemia (RadLV) in C57Bl mice and two types derived therefrom by serial passages in mice (RadLV-RS) and in rats (RadLV-rat) were studied. Whereas the p 30 from RLV or RadLV (rat) viruses showed complete identity, the cross-reaction of their gp 70 proteins wasonly partial. The main findings in the tissues were the following: 1. The concentration of p 30 and gp 70 antigens increased much more during the RadLV-rat leukemia than in irradiated or RadLV (RS) treated mice. 2. In the serum, the ratio p 30/gp 70 was in general higher than one in the Rauscher leukemic mice and less than one in all types of RadLV leukemias. 3. In the spleen, RLV and RadLV (RS) infected mice had higher levels of p 30 than of gp 70. The reverse occurred in irradiated mice and in leukemic rats. Finally, the catabolic degradation of labeled RLV gp 70 was similar in normal and leukemic mice."}
{"id": "PMID:486688", "title": "Effects of ornithine alphaketoglutarate on blood insulin, glucagon and aminoacids in alcoholic cirrhosis.", "content": "After summing up existing theories about the origins and development of functional hepatic encephalopathy, the authors report on the effects of six-hour intravenous infusions of ornithine alphaketoglutarate (60 g dissolved in 500 ml distilled water), administered to 10 patients with ethylic hepatic cirrhosis in conjunction with a normal protein intake (70 g/day). Arterial blood ammonemia, venous blood aminoacidemia and the insulin/glucagon ratio did not vary during or after infusion. This method of treatment therefore seems to meet the protein requirements of these undernourished patients.", "contents": "Effects of ornithine alphaketoglutarate on blood insulin, glucagon and aminoacids in alcoholic cirrhosis. After summing up existing theories about the origins and development of functional hepatic encephalopathy, the authors report on the effects of six-hour intravenous infusions of ornithine alphaketoglutarate (60 g dissolved in 500 ml distilled water), administered to 10 patients with ethylic hepatic cirrhosis in conjunction with a normal protein intake (70 g/day). Arterial blood ammonemia, venous blood aminoacidemia and the insulin/glucagon ratio did not vary during or after infusion. This method of treatment therefore seems to meet the protein requirements of these undernourished patients."}
{"id": "PMID:486689", "title": "Humoral regulation of pluripotent stem cell differentiation.", "content": "Humoral regulators of CFU-S differentiation have been demonstrated in bone marrow of Ara-C treated animals. These factors are effective in vivo and are also capable of inducing erythropoietic differentiation of normal CFU-S in vitro. These results seem to indicate that the microenvironment acts at the committed stem cell level while the factors described in this paper act on the pluripotent stem cells.", "contents": "Humoral regulation of pluripotent stem cell differentiation. Humoral regulators of CFU-S differentiation have been demonstrated in bone marrow of Ara-C treated animals. These factors are effective in vivo and are also capable of inducing erythropoietic differentiation of normal CFU-S in vitro. These results seem to indicate that the microenvironment acts at the committed stem cell level while the factors described in this paper act on the pluripotent stem cells."}
{"id": "PMID:486691", "title": "Spontaneous rosette formation in patients with oral cancer.", "content": "The percentage of \"total\" E-rosettes was studied in the peripheral blood of 38 untreated patients with squamous cell carcinoma of the oral cavity, performing the test at 4 degrees C and at 20 degrees C. At 4 degrees C the quantity of the E-rosettes was higher than at 20 degrees C. The mean value of E-rosettes was strongly reduced only in 17 of these patients with metastases in the regional lymph nodes when compared with a group of 40 normal individuals as well as with the group of remaining 21 patients with localized cancer (P less than 0.001), whatever the temperature of testing. The mean percentage of 20 degrees C E-rosettes was significantly higher in patients with localized disease than in the 22 normal donors (50.2 +/- 3.0% vs 41.8 +/- 2.0%, P less than 0.01) but in favour of 4 degrees C E-rosettes the difference was not significant (57.3 +/- 2.8% vs 54.2 +/- 2.9%). Thus there was a clear correlation between changes of the T cell level and the clinical stage of the disease.", "contents": "Spontaneous rosette formation in patients with oral cancer. The percentage of \"total\" E-rosettes was studied in the peripheral blood of 38 untreated patients with squamous cell carcinoma of the oral cavity, performing the test at 4 degrees C and at 20 degrees C. At 4 degrees C the quantity of the E-rosettes was higher than at 20 degrees C. The mean value of E-rosettes was strongly reduced only in 17 of these patients with metastases in the regional lymph nodes when compared with a group of 40 normal individuals as well as with the group of remaining 21 patients with localized cancer (P less than 0.001), whatever the temperature of testing. The mean percentage of 20 degrees C E-rosettes was significantly higher in patients with localized disease than in the 22 normal donors (50.2 +/- 3.0% vs 41.8 +/- 2.0%, P less than 0.01) but in favour of 4 degrees C E-rosettes the difference was not significant (57.3 +/- 2.8% vs 54.2 +/- 2.9%). Thus there was a clear correlation between changes of the T cell level and the clinical stage of the disease."}
{"id": "PMID:486690", "title": "Effect of a sarcolysin-containing polymer on the the lymphatic tissues and immune response to sheep red blood cells.", "content": "The influence on the size of lymphoid organs and immune response to sheep erythrocytes in BALB/c mice of an effective antitumor polymer preparation, a compound of sarcolysin with a copolymer of vinylpyrrolidone and maleic anhydride (SVPMA) was studied. A significant reduction in the weights of the thymus and spleen, and in the serum hemagglutinin titers as well as a slight decrease in the number of spleen rosette-forming cells were observed on animals receiving sarcolysin rather than on animals receiving SVPMA when compared with each other as well as with a control group of mice. Moreover a significant stimulating effect on the size of the lymph nodes and partly on the spleen was seen in the mice treated with SVPMA. These results, therefore, provide additional evidence on the possibility to obtain, on the basis of synthetic polymers, effective antitumor preparations with minimal or no immunodepressive properties.", "contents": "Effect of a sarcolysin-containing polymer on the the lymphatic tissues and immune response to sheep red blood cells. The influence on the size of lymphoid organs and immune response to sheep erythrocytes in BALB/c mice of an effective antitumor polymer preparation, a compound of sarcolysin with a copolymer of vinylpyrrolidone and maleic anhydride (SVPMA) was studied. A significant reduction in the weights of the thymus and spleen, and in the serum hemagglutinin titers as well as a slight decrease in the number of spleen rosette-forming cells were observed on animals receiving sarcolysin rather than on animals receiving SVPMA when compared with each other as well as with a control group of mice. Moreover a significant stimulating effect on the size of the lymph nodes and partly on the spleen was seen in the mice treated with SVPMA. These results, therefore, provide additional evidence on the possibility to obtain, on the basis of synthetic polymers, effective antitumor preparations with minimal or no immunodepressive properties."}
{"id": "PMID:486692", "title": "In vitro estimation of the proliferative state of murine bone marrow \"colony forming units in culture\" (CFUc) with hydroxyurea (HU).", "content": "Exposure of CFUc to 1.0 mM of HU for two hours was at the plateau of both the dose--and time--respose curves, killing 38% of the CFUc. This is compatible with the result of the 3HTdR \"suicide\" method, a standard procedure for the estimation of the proportion of CFUc being in the S phase.", "contents": "In vitro estimation of the proliferative state of murine bone marrow \"colony forming units in culture\" (CFUc) with hydroxyurea (HU). Exposure of CFUc to 1.0 mM of HU for two hours was at the plateau of both the dose--and time--respose curves, killing 38% of the CFUc. This is compatible with the result of the 3HTdR \"suicide\" method, a standard procedure for the estimation of the proportion of CFUc being in the S phase."}
{"id": "PMID:486693", "title": "The histological changes in the liver, lung and kidney after scorpion poisoning (Buthus quinquestriatus).", "content": "The effect of sublethal doses of Buthus quinquestriatus on the hepatic vascular bed and hepatic parenchyma were studied. The main effect of the venom was primarily on the vascular bed of the liver as manifested by dilatation of branches of the hepatic artery, portal vein together with intravascular thrombi and subcapsular haemorrhages. Apart from mild hydropic degeneration some cells of the hepatic parenchyma showed focal necrosis and Kupffer cells were frequently hypertrophied and contained pigment. In the lung, dilated thrombosed vessels were observed. In the kidney, the vessels showed the smae changes. The epithelial and endothelial cells of the glomeruli appeared normal except for slight swelling with intact basement membrane. The tubular cells were swollen with increased granularity and attenuation of their tubular lumen, a picture compatible with hydropic degeneration. The possible mechanisms in inducing such lesions were discussed.", "contents": "The histological changes in the liver, lung and kidney after scorpion poisoning (Buthus quinquestriatus). The effect of sublethal doses of Buthus quinquestriatus on the hepatic vascular bed and hepatic parenchyma were studied. The main effect of the venom was primarily on the vascular bed of the liver as manifested by dilatation of branches of the hepatic artery, portal vein together with intravascular thrombi and subcapsular haemorrhages. Apart from mild hydropic degeneration some cells of the hepatic parenchyma showed focal necrosis and Kupffer cells were frequently hypertrophied and contained pigment. In the lung, dilated thrombosed vessels were observed. In the kidney, the vessels showed the smae changes. The epithelial and endothelial cells of the glomeruli appeared normal except for slight swelling with intact basement membrane. The tubular cells were swollen with increased granularity and attenuation of their tubular lumen, a picture compatible with hydropic degeneration. The possible mechanisms in inducing such lesions were discussed."}
{"id": "PMID:486694", "title": "PBG-synthetase activity in the liver of Yoshida sarcoma-bearing rats.", "content": "The etiology of anemia in cancer is not fully understood. A possible cause of the anemia of tumor-bearing animals could be a decreased activity in the enzymes of the heme-pathway. We report the enzyme activity of porphobilinogen-synthetase in the liver of Yoshida sarcoma-bearing rats. PBG-synthetase activity in the whole liver, is higher in tumor-bearing rats than in controls, although the enzyme activity by a gram of wet liver is decreased. Hence PBG-synthetase activity is not a limiting factor in the biosynthesis of heme in tumor-bearing animals.", "contents": "PBG-synthetase activity in the liver of Yoshida sarcoma-bearing rats. The etiology of anemia in cancer is not fully understood. A possible cause of the anemia of tumor-bearing animals could be a decreased activity in the enzymes of the heme-pathway. We report the enzyme activity of porphobilinogen-synthetase in the liver of Yoshida sarcoma-bearing rats. PBG-synthetase activity in the whole liver, is higher in tumor-bearing rats than in controls, although the enzyme activity by a gram of wet liver is decreased. Hence PBG-synthetase activity is not a limiting factor in the biosynthesis of heme in tumor-bearing animals."}
{"id": "PMID:486695", "title": "The value of serum alkaline phosphatase alpha 1 isoenzyme in the diagnosis of liver metastases. Preliminary results.", "content": "Serum alkaline phosphatase isoenzyme electrophoresis on cellulose acetate plates in a normal population of 91 controls and 255 cancer patients demonstrates the presence of an isoenzyme alpha 1 in 36 out of39 cases of confirmed liver metastases. In 188 cancer patients without apparent hepatic lesion and with a normal liver biology, the alpha 1 isoenzyme has been found in 45 cases, but 11 presented afterwards confirmed liver metastases. This isoenzyme remained negative in the normal population. Therefore, our results indicate that the alpha 1 alkaline phosphatase isoenzyme may be a useful marker for the biological diagnosis of liver metastases.", "contents": "The value of serum alkaline phosphatase alpha 1 isoenzyme in the diagnosis of liver metastases. Preliminary results. Serum alkaline phosphatase isoenzyme electrophoresis on cellulose acetate plates in a normal population of 91 controls and 255 cancer patients demonstrates the presence of an isoenzyme alpha 1 in 36 out of39 cases of confirmed liver metastases. In 188 cancer patients without apparent hepatic lesion and with a normal liver biology, the alpha 1 isoenzyme has been found in 45 cases, but 11 presented afterwards confirmed liver metastases. This isoenzyme remained negative in the normal population. Therefore, our results indicate that the alpha 1 alkaline phosphatase isoenzyme may be a useful marker for the biological diagnosis of liver metastases."}
{"id": "PMID:486696", "title": "A comparative study of the Coulter Counter Model S, for the enumeration of peripheral leukocyte counts below 3.0 x 10(9)/l using whole blood and prediluted blood.", "content": "In this study low leukocyte counts were carried out using several methods. The results obtained by the visual method, the Coulter Model \"S\" using \"whole blood\", and the Coulter DN, show close correlation, but discrepancies exist when the Coulter S uses \"prediluted blood\". This is explained by excessive bubbles. This does not allow the Coulter Model S to be used with confidence for low leukocyte counts if \"whole blood\" is not available.", "contents": "A comparative study of the Coulter Counter Model S, for the enumeration of peripheral leukocyte counts below 3.0 x 10(9)/l using whole blood and prediluted blood. In this study low leukocyte counts were carried out using several methods. The results obtained by the visual method, the Coulter Model \"S\" using \"whole blood\", and the Coulter DN, show close correlation, but discrepancies exist when the Coulter S uses \"prediluted blood\". This is explained by excessive bubbles. This does not allow the Coulter Model S to be used with confidence for low leukocyte counts if \"whole blood\" is not available."}
{"id": "PMID:486698", "title": "Electric birefringence of DNA and chromatin. Influence of divalent cations.", "content": "The effects of divalent cations on the DNA and chromatin conformation have been investigated by electric birefringence and birefringence relaxation measurements at low and constant ionic strength (0.001). An important decrease of the intrinsic optical anisotropy of DNA has been found in the presence of Mn2+ and Cu2+, but not with Mg2+. A complex variation of the mean relaxation time with the ratio I/P of ion to DNA-phosphate molar concentration has been evidenced in the presence of Mn2+ and Cu2+, while the mean relaxation time monotonously decreased in the presence of Mg2+. These observations are interpreted in terms of a specific organization of DNA in a compact, rigid structure, in the presence of Mn2+ and Cu2+, and a non-specific coiling in the presence of Mg2+. Drastic conformational changes encountered by chromatin in the presence of Mg2+ and Mn2+ cations have also been evidenced through electric birefringence measurements. They are interpreted by the formation of a superhelical compact arrangement of nucleosome strings which yielded a reversal of the birefringence sign with respect to the negative anisotropy observed in the presence of Na+ ions. The removal of the histone H1 prevented the appearance of this quaternary structure. More extended fragments of the chromatin chain obtained by ECTHAM chromatography of sonicated chromatin could not afford such compact arrangements.", "contents": "Electric birefringence of DNA and chromatin. Influence of divalent cations. The effects of divalent cations on the DNA and chromatin conformation have been investigated by electric birefringence and birefringence relaxation measurements at low and constant ionic strength (0.001). An important decrease of the intrinsic optical anisotropy of DNA has been found in the presence of Mn2+ and Cu2+, but not with Mg2+. A complex variation of the mean relaxation time with the ratio I/P of ion to DNA-phosphate molar concentration has been evidenced in the presence of Mn2+ and Cu2+, while the mean relaxation time monotonously decreased in the presence of Mg2+. These observations are interpreted in terms of a specific organization of DNA in a compact, rigid structure, in the presence of Mn2+ and Cu2+, and a non-specific coiling in the presence of Mg2+. Drastic conformational changes encountered by chromatin in the presence of Mg2+ and Mn2+ cations have also been evidenced through electric birefringence measurements. They are interpreted by the formation of a superhelical compact arrangement of nucleosome strings which yielded a reversal of the birefringence sign with respect to the negative anisotropy observed in the presence of Na+ ions. The removal of the histone H1 prevented the appearance of this quaternary structure. More extended fragments of the chromatin chain obtained by ECTHAM chromatography of sonicated chromatin could not afford such compact arrangements."}
{"id": "PMID:486699", "title": "Electric permittivity and dielectric dispersion of low-molecular weight DNA at low ionic strength.", "content": "The dielectric properties of sonicated calf-thymus DNA (MW approximately 3 X 10(5) g mol-1) have been investigated in a frequency range between a few kHz and 100 MHz. Two samples, sonicated in a different way were used after proper characterization including light-scattering, viscometry and contour length distribution by electron microscopy. Dielectric measurements were performed at several concentrations between 10(-4) and 3 X 10(-3) monomol 1-1 and 22 degrees C. Under all circumstances two separated dispersion regions were observed, the corresponding specific increments of which decreased with increasing concentration. The same was observed with the mean relaxation time of the high frequency dispersion. Both the frequency and concentration dependence was largely analogous to what is observed with other polyelectrolytes. Values of the dielectric parameters extrapolated to infinite dilution could also be interpreted in the same manner as for more simple, charged macromolecules and no specific effects had to be taken into account.", "contents": "Electric permittivity and dielectric dispersion of low-molecular weight DNA at low ionic strength. The dielectric properties of sonicated calf-thymus DNA (MW approximately 3 X 10(5) g mol-1) have been investigated in a frequency range between a few kHz and 100 MHz. Two samples, sonicated in a different way were used after proper characterization including light-scattering, viscometry and contour length distribution by electron microscopy. Dielectric measurements were performed at several concentrations between 10(-4) and 3 X 10(-3) monomol 1-1 and 22 degrees C. Under all circumstances two separated dispersion regions were observed, the corresponding specific increments of which decreased with increasing concentration. The same was observed with the mean relaxation time of the high frequency dispersion. Both the frequency and concentration dependence was largely analogous to what is observed with other polyelectrolytes. Values of the dielectric parameters extrapolated to infinite dilution could also be interpreted in the same manner as for more simple, charged macromolecules and no specific effects had to be taken into account."}
{"id": "PMID:486700", "title": "Inhibition of acetylcholinesterase by TX-100.", "content": "At high detergent concentrations, approximately the equivalent of 2 micelles of TX-100 reversibly bind to acetylcholinesterase and fully inhibit the enzyme. This result suggests that the appropriate lipid environment might regulate this neuronal enzyme's function.", "contents": "Inhibition of acetylcholinesterase by TX-100. At high detergent concentrations, approximately the equivalent of 2 micelles of TX-100 reversibly bind to acetylcholinesterase and fully inhibit the enzyme. This result suggests that the appropriate lipid environment might regulate this neuronal enzyme's function."}
{"id": "PMID:486701", "title": "Selection and self-organization of self-reproducing macromolecules under the constraint of constant flux.", "content": "We investigate the dynamic behavior of a set of self-reproducing macromolecules (e.g., polynucleotides) under conditions such that the fluxes of all monomer units into the system are kept constant. Such conditions might prevail in an evolution reactor or in certain naturally occurring situations. A general set of equations is developed to describe the behavior of both the macromolecule and the monomer concentrations. The question of how the rate of macromolecule synthesis varies with the monomer levels is discussed briefly. With the help of several physically reasonable approximations, we obtain an exact solution for a simplified constant flux system. Comparison with the corresponding system under the constraint of constant overall organization reveals important similarities, most notably in the existance and composition of quasispecies. Given the same set of physical and chemical parameters, a system subject to constant flux will always evolve toward selective equilibrium more slowly than under the constraint of constant organization.", "contents": "Selection and self-organization of self-reproducing macromolecules under the constraint of constant flux. We investigate the dynamic behavior of a set of self-reproducing macromolecules (e.g., polynucleotides) under conditions such that the fluxes of all monomer units into the system are kept constant. Such conditions might prevail in an evolution reactor or in certain naturally occurring situations. A general set of equations is developed to describe the behavior of both the macromolecule and the monomer concentrations. The question of how the rate of macromolecule synthesis varies with the monomer levels is discussed briefly. With the help of several physically reasonable approximations, we obtain an exact solution for a simplified constant flux system. Comparison with the corresponding system under the constraint of constant overall organization reveals important similarities, most notably in the existance and composition of quasispecies. Given the same set of physical and chemical parameters, a system subject to constant flux will always evolve toward selective equilibrium more slowly than under the constraint of constant organization."}
{"id": "PMID:486702", "title": "Statistical-mechanical theory of passive transport through semipermeable membranes.", "content": "The first general multicomponent equations for transport through semipermeable membranes are derived from basic statistical-mechanical principles. The procedure follows that used earlier for open membranes, but semipermeability is modelled mathematically by the introduction of external forces on the impermeant species. Gases are treated first in order to clarify the problems involved, but the final results apply to general nonideal solutions of any concentration. The mixed-solvent effect is treated rigorously, and a mixed-solvent osmotic pressure is defined. A useful specific identification of so-called osmotic flow is given, along with a demonstration that such an identification cannot be unique. Results are obtained both for discontinuous membrane models, and for a continuous model.", "contents": "Statistical-mechanical theory of passive transport through semipermeable membranes. The first general multicomponent equations for transport through semipermeable membranes are derived from basic statistical-mechanical principles. The procedure follows that used earlier for open membranes, but semipermeability is modelled mathematically by the introduction of external forces on the impermeant species. Gases are treated first in order to clarify the problems involved, but the final results apply to general nonideal solutions of any concentration. The mixed-solvent effect is treated rigorously, and a mixed-solvent osmotic pressure is defined. A useful specific identification of so-called osmotic flow is given, along with a demonstration that such an identification cannot be unique. Results are obtained both for discontinuous membrane models, and for a continuous model."}
{"id": "PMID:486703", "title": "Self-association of lyophilized horse liver alcohol dehydrogenase.", "content": "The molecular weights of lyophilized and non-lyophilized horse liver alcohol dehydrogenase have been compared by quasi-elastic light scattering, and ultracentrifugation. Whereas the non-lyophilized enzyme has the expected molecular weight of 78 000, the lyophilized enz)me has an initial molecular weight of about 10(6) which increases with time by an endothermic process. This result shows that any physical measurement using lyophilized liver alcohol dehydrogenase to investigate the enzyme mechanism, which relies upon the molecular size, will be invalid.", "contents": "Self-association of lyophilized horse liver alcohol dehydrogenase. The molecular weights of lyophilized and non-lyophilized horse liver alcohol dehydrogenase have been compared by quasi-elastic light scattering, and ultracentrifugation. Whereas the non-lyophilized enzyme has the expected molecular weight of 78 000, the lyophilized enz)me has an initial molecular weight of about 10(6) which increases with time by an endothermic process. This result shows that any physical measurement using lyophilized liver alcohol dehydrogenase to investigate the enzyme mechanism, which relies upon the molecular size, will be invalid."}
{"id": "PMID:486704", "title": "Molecular mechanism for the initial process of visual excitation. IV. Energy surfaces of visual pigments and photoisomerization mechanism.", "content": "Using the twisted conformations of the chromophores for visual pigments and intermediates which were theoretically determined in the previous paper, energy surfaces of the pigment at - 190 degrees C were obtained as functions of the torsional angles theta 9-10 and theta 11-12 or of the torsional angles theta 9-10 and theta 13-14. In these calculations, the existence of specific reaction paths between rhodopsin (R) and bathorhodopsin (B), between isorhodopsin I (I) and bathorhodopsin, and between isorhodopsin II (I') and bathorhodopsin were assumed. It was shown that the total energy surfaces of the excited states had minima C1 at theta 9-10 approximately -10 degrees and theta 11-12 approximately -80 degrees, C2 at theta 9-10 approximately -85 degrees and theta 11-12 approximately -5 degrees, and C3 at theta 9-10 approximately -0 degree and theta 13-14 approximately -90 degrees. These minima are considered to correspond to the thermally barrierless common states as denoted by Rosenfeld et al. Using the total energy surfaces in the ground and excited states, the molecular mechanism of the photoisomerization reaction was suggested. Quantum yields for the photoconversions among R, I, I' and B were related to the rates of vibrational relaxations, radiationless transitions and thermal excitations. Some discussion was made of the temperature effect on the quantum yield. Similar calculations of the energy surfaces were also made at other temperatures where lumirhodopsin or metarhodopsin I is stable. Relative energy levels of the pigments and the intermediates were discussed.", "contents": "Molecular mechanism for the initial process of visual excitation. IV. Energy surfaces of visual pigments and photoisomerization mechanism. Using the twisted conformations of the chromophores for visual pigments and intermediates which were theoretically determined in the previous paper, energy surfaces of the pigment at - 190 degrees C were obtained as functions of the torsional angles theta 9-10 and theta 11-12 or of the torsional angles theta 9-10 and theta 13-14. In these calculations, the existence of specific reaction paths between rhodopsin (R) and bathorhodopsin (B), between isorhodopsin I (I) and bathorhodopsin, and between isorhodopsin II (I') and bathorhodopsin were assumed. It was shown that the total energy surfaces of the excited states had minima C1 at theta 9-10 approximately -10 degrees and theta 11-12 approximately -80 degrees, C2 at theta 9-10 approximately -85 degrees and theta 11-12 approximately -5 degrees, and C3 at theta 9-10 approximately -0 degree and theta 13-14 approximately -90 degrees. These minima are considered to correspond to the thermally barrierless common states as denoted by Rosenfeld et al. Using the total energy surfaces in the ground and excited states, the molecular mechanism of the photoisomerization reaction was suggested. Quantum yields for the photoconversions among R, I, I' and B were related to the rates of vibrational relaxations, radiationless transitions and thermal excitations. Some discussion was made of the temperature effect on the quantum yield. Similar calculations of the energy surfaces were also made at other temperatures where lumirhodopsin or metarhodopsin I is stable. Relative energy levels of the pigments and the intermediates were discussed."}
{"id": "PMID:486705", "title": "The directional absorption properties of rhodopsin and its photoproducts.", "content": "The experimental data on the absorption of a plane polarised light by a solution of cattle rhodopsin at -196 degrees C have been theoretically analysed to model the directional absorption properties of rhodopsin and its photoproducts. It is seen that these molecules behave like planar absorbers having a ratio of about 100 : 7 between the extinction coefficients along the long axis and perpendicular to it. Using this result and the experimental observations on absorption and dichroism in the retina in situ, a model for the configuration of chromophores in the disc membranes has been derived. In this model the plane of the chromophore is perpendicular to that of the disc and the long axis of the chromophore makes an angle of 6.6 degrees with the plane of the disc. The solution of the problem depends on the assumption that the absorption axes are the same for the rhodopsin, prelumirhodopsin and isorhodopsin.", "contents": "The directional absorption properties of rhodopsin and its photoproducts. The experimental data on the absorption of a plane polarised light by a solution of cattle rhodopsin at -196 degrees C have been theoretically analysed to model the directional absorption properties of rhodopsin and its photoproducts. It is seen that these molecules behave like planar absorbers having a ratio of about 100 : 7 between the extinction coefficients along the long axis and perpendicular to it. Using this result and the experimental observations on absorption and dichroism in the retina in situ, a model for the configuration of chromophores in the disc membranes has been derived. In this model the plane of the chromophore is perpendicular to that of the disc and the long axis of the chromophore makes an angle of 6.6 degrees with the plane of the disc. The solution of the problem depends on the assumption that the absorption axes are the same for the rhodopsin, prelumirhodopsin and isorhodopsin."}
{"id": "PMID:486706", "title": "Influence of the 6-trimethylsilyl group on the fragmentation of the trimethylsilyl derivatives of some 6-hydroxy- and 3,6-dihydroxy-steroids and related compounds.", "content": "The 25 eV mass spectra of the trimethylsilyl derivatives of a number of 6-hydroxy and 3,6-dihydroxy steroids together with deuterium and 18O-labeled analogs have been examined to determine the influence of the 6-OTMS group on fragmentation patterns. Ions in the cholestane series at m/z 321 and 403 were the most characteristic ions derived from the 6-OTMS function; their relative abundances, although low in the spectra of 6-OTMS steroids themselves, were considerably elevated when a 3-OTMS or 3-oxo group was present. Similar ions were present in the spectra of androstane and pregnane derivatives. No correlation was found between the abundance of these ions and the stereochemistry at C3, C5, or C6. Fragmentation mechanisms and gas chromatographic data are discussed.", "contents": "Influence of the 6-trimethylsilyl group on the fragmentation of the trimethylsilyl derivatives of some 6-hydroxy- and 3,6-dihydroxy-steroids and related compounds. The 25 eV mass spectra of the trimethylsilyl derivatives of a number of 6-hydroxy and 3,6-dihydroxy steroids together with deuterium and 18O-labeled analogs have been examined to determine the influence of the 6-OTMS group on fragmentation patterns. Ions in the cholestane series at m/z 321 and 403 were the most characteristic ions derived from the 6-OTMS function; their relative abundances, although low in the spectra of 6-OTMS steroids themselves, were considerably elevated when a 3-OTMS or 3-oxo group was present. Similar ions were present in the spectra of androstane and pregnane derivatives. No correlation was found between the abundance of these ions and the stereochemistry at C3, C5, or C6. Fragmentation mechanisms and gas chromatographic data are discussed."}
{"id": "PMID:486707", "title": "Separation and determination of oleic and cis-vaccenic acids by gas-liquid chromatography mass spectrometry using a polar cyanopropylsiloxane liquid phase.", "content": "The separation and quantification of the monoenoic isomeric oleic (cis-9-octadecenoic) and cis-vaccenic (cis-11-octadecenoic) acids as their pyrrolidine derivatives, was studied with a gas chromatograph mass spectrometer system using mass chromatography. By monitoring the characteristic ions of the pyrrolidine derivatives of oleic (m/z 208 and 222) and cis-vaccenic (m/z 210 and 224) acids, both derivatives were separated. The ratio of m/z 210 to 208 was proportional to the relative amounts of cis-vaccenic to oleic acid. By this method, the content of cis-vaccenic acid in choline phosphoglycerides from rat liver and Yoshida ascites hepatoma (AH 7974) was determined.", "contents": "Separation and determination of oleic and cis-vaccenic acids by gas-liquid chromatography mass spectrometry using a polar cyanopropylsiloxane liquid phase. The separation and quantification of the monoenoic isomeric oleic (cis-9-octadecenoic) and cis-vaccenic (cis-11-octadecenoic) acids as their pyrrolidine derivatives, was studied with a gas chromatograph mass spectrometer system using mass chromatography. By monitoring the characteristic ions of the pyrrolidine derivatives of oleic (m/z 208 and 222) and cis-vaccenic (m/z 210 and 224) acids, both derivatives were separated. The ratio of m/z 210 to 208 was proportional to the relative amounts of cis-vaccenic to oleic acid. By this method, the content of cis-vaccenic acid in choline phosphoglycerides from rat liver and Yoshida ascites hepatoma (AH 7974) was determined."}
{"id": "PMID:486708", "title": "Some new fluorescent derivatives for the mass spectrometric quantitation of biogenic amines.", "content": "The 5-dimethyl-, diethyl-, dipropyl-, dibutyl-, and dipentyl-aminoaphghalene-1-sulfonyl (dansyl, ethanyl, propansyl, bansyl and pentansyl respectively) derivatives of tyramine and other biogenic amines were prepared and their mass spectra recorded. The relative intensity of the largest unique ion increased with increasing length of the alkyl group. Several O-alkyl-N-propansyl- and N,O-dialkyl-N-propansyl-, bansyl- and pentansyl-tyramines were also synthesized and their mass spectra recorded. Dimethylbansyl- and dimethylpentansyl-tyramines exhibited the largest unique ions in their mass spectra and the greatest sensitivity in quantitation by the integrated ion current method. Procedures for preparing these derivatives in amounts ranging from nanograms to milligrams are presented and their thin-layer chromatographic behavior in three solvent systems is described.", "contents": "Some new fluorescent derivatives for the mass spectrometric quantitation of biogenic amines. The 5-dimethyl-, diethyl-, dipropyl-, dibutyl-, and dipentyl-aminoaphghalene-1-sulfonyl (dansyl, ethanyl, propansyl, bansyl and pentansyl respectively) derivatives of tyramine and other biogenic amines were prepared and their mass spectra recorded. The relative intensity of the largest unique ion increased with increasing length of the alkyl group. Several O-alkyl-N-propansyl- and N,O-dialkyl-N-propansyl-, bansyl- and pentansyl-tyramines were also synthesized and their mass spectra recorded. Dimethylbansyl- and dimethylpentansyl-tyramines exhibited the largest unique ions in their mass spectra and the greatest sensitivity in quantitation by the integrated ion current method. Procedures for preparing these derivatives in amounts ranging from nanograms to milligrams are presented and their thin-layer chromatographic behavior in three solvent systems is described."}
{"id": "PMID:486709", "title": "Methane-oxygen enhanced negative ion mass spectra of polychlorinated diphenyl ethers.", "content": "Methane-oxygen enhanced negative ion mass spectra of 15 chlorinated diphenyl ethers contained some combination of [M-]-., [M-1]-, [M-19]-, [M-36]-, [M-55]-, and [M+35]- anions. The relative abundances of these ions are related to the chlorine substitution pattern. Similar ions appear in the negative ion spectra of polychlorinated anisoles. The study of known isomers of polychlorinated model compounds extends the utility of the negative ion method in the analysis of polychlorinated aromatics such as polychlorinated biphenyls, dioxins and furans. The spectra were shown to be very dependent on the temperature of the ion source; a temperature change of 25 degrees C was shown to have pronounced effects. Spectral variation with temperature has implications for computer library searches, ultimate sensitivity limits and analysis in the presence of interfering isobaric ions.", "contents": "Methane-oxygen enhanced negative ion mass spectra of polychlorinated diphenyl ethers. Methane-oxygen enhanced negative ion mass spectra of 15 chlorinated diphenyl ethers contained some combination of [M-]-., [M-1]-, [M-19]-, [M-36]-, [M-55]-, and [M+35]- anions. The relative abundances of these ions are related to the chlorine substitution pattern. Similar ions appear in the negative ion spectra of polychlorinated anisoles. The study of known isomers of polychlorinated model compounds extends the utility of the negative ion method in the analysis of polychlorinated aromatics such as polychlorinated biphenyls, dioxins and furans. The spectra were shown to be very dependent on the temperature of the ion source; a temperature change of 25 degrees C was shown to have pronounced effects. Spectral variation with temperature has implications for computer library searches, ultimate sensitivity limits and analysis in the presence of interfering isobaric ions."}
{"id": "PMID:486710", "title": "The electron impact mass spectra of some clinically important androsterones and androsterols as their formate derivatives.", "content": "The electron impact mass spectra of a number of androsterones and androsterols as their formate ester derivatives are discussed; these ester derivatives are in common use for routine assay of 17-oxosteroids and 17-oxogenic steroids in biological samples.", "contents": "The electron impact mass spectra of some clinically important androsterones and androsterols as their formate derivatives. The electron impact mass spectra of a number of androsterones and androsterols as their formate ester derivatives are discussed; these ester derivatives are in common use for routine assay of 17-oxosteroids and 17-oxogenic steroids in biological samples."}
{"id": "PMID:486711", "title": "Inexpensive analog equipment for processing gas chromatography mass spectrometry data.", "content": "Using relatively simple analog circuitry, and an inexpensive analog frequency modulated tape recorder (or a modified audio recorder) for data storage, repetitively scanned spectra may be examined on an oscilloscope, and reconstructed mass chromatograms produced. The same equipment may be used for peak matching, and for selective ion detection over the full mass range of a sector instrument.", "contents": "Inexpensive analog equipment for processing gas chromatography mass spectrometry data. Using relatively simple analog circuitry, and an inexpensive analog frequency modulated tape recorder (or a modified audio recorder) for data storage, repetitively scanned spectra may be examined on an oscilloscope, and reconstructed mass chromatograms produced. The same equipment may be used for peak matching, and for selective ion detection over the full mass range of a sector instrument."}
{"id": "PMID:486712", "title": "The effect of crystal size on the bioavailability of benoxaprofen: studies utilizing deuterium labeled drug.", "content": "A study of the effect of crystal size on the bioavailability of benoxaprofen, 2-[4-chlorophenyl]-alpha-methyl-5-benzoxazoleacetic acid, in man is reported. The technique utilized comparison of either the plasma concentrations or urine levels, resulting from administration of deuterium labeled (2H7) drug in solution coadministered with a test capsule formulation. Drug concentrations were determined by gas chromatography, and the ratio of labeled to unlabeled drug was obtained by gas chromatography mass spectrometry. Measurements following coadministration of labeled and unlabeled drug in solution established the absence of an isotope effect due to the presence of deuterium. The dry formulations consisted of either a 3.17--100 micron fraction (mean = 18.5 microns) or a 32--1000 micron fraction (mean = 610 microns) formulated with starch powder. The results in three subjects indicate an almost complete availability (0.95--0.98) of the small crystals as measured by comparison of either area under the plasma level curves or urine excretion (0.94--0.97) of labeled versus unlabeled drug measured to 168 hours. The larger crystals exhibited a lower availability as shown by plasma levels (0.41--0.46) or urine recovery (0.39--0.43). A higher dose of the large crystal formulation resulted in decreased relative availability with a fourfold dose dropping availability to 0.22 in a single subject.", "contents": "The effect of crystal size on the bioavailability of benoxaprofen: studies utilizing deuterium labeled drug. A study of the effect of crystal size on the bioavailability of benoxaprofen, 2-[4-chlorophenyl]-alpha-methyl-5-benzoxazoleacetic acid, in man is reported. The technique utilized comparison of either the plasma concentrations or urine levels, resulting from administration of deuterium labeled (2H7) drug in solution coadministered with a test capsule formulation. Drug concentrations were determined by gas chromatography, and the ratio of labeled to unlabeled drug was obtained by gas chromatography mass spectrometry. Measurements following coadministration of labeled and unlabeled drug in solution established the absence of an isotope effect due to the presence of deuterium. The dry formulations consisted of either a 3.17--100 micron fraction (mean = 18.5 microns) or a 32--1000 micron fraction (mean = 610 microns) formulated with starch powder. The results in three subjects indicate an almost complete availability (0.95--0.98) of the small crystals as measured by comparison of either area under the plasma level curves or urine excretion (0.94--0.97) of labeled versus unlabeled drug measured to 168 hours. The larger crystals exhibited a lower availability as shown by plasma levels (0.41--0.46) or urine recovery (0.39--0.43). A higher dose of the large crystal formulation resulted in decreased relative availability with a fourfold dose dropping availability to 0.22 in a single subject."}
{"id": "PMID:486713", "title": "Aporphines. 25-trimethylislyl derivatives of N-methyl and N-propyl aporphines: gas chromatographic and mass spectrometric properties.", "content": "A series of synthetic, semisynthetic and naturally occurring aporphine alkaloids were converted to the O-trimethylsilyl derivatives, and analyzed by gas chromatography mass spectrometry. A discussion of structurally informative fragment ions along with gas chromatographic data is presented. Their applicability to the analysis of biological metabolites of apomorphine, a CNS active structural analog of dopamine, is discussed.", "contents": "Aporphines. 25-trimethylislyl derivatives of N-methyl and N-propyl aporphines: gas chromatographic and mass spectrometric properties. A series of synthetic, semisynthetic and naturally occurring aporphine alkaloids were converted to the O-trimethylsilyl derivatives, and analyzed by gas chromatography mass spectrometry. A discussion of structurally informative fragment ions along with gas chromatographic data is presented. Their applicability to the analysis of biological metabolites of apomorphine, a CNS active structural analog of dopamine, is discussed."}
{"id": "PMID:486714", "title": "The effects of heating rate and sample size on the direct exposure/chemical ionization mass spectra of some biological conjugates.", "content": "A direct exposure probe has been used to obtain mass spectra of underivatized guanosine, deoxyguanosine, sucrose and the p-nitrophenyl-beta-D-glucuronide. In all cases, a protonated molecular ion is produced with good relative abundance. The effects of heating rate and sample size on the production of the [MH]+ ION ARE EXAMINED IN DETAIL FROM TOTAL ION ANd single ion currents produced during rapid, repetitive scanning of the spectra after probe insertion. From this data we conclude that protonated molecular ions are produced as a result of the enhanced volatility of neutral molecules on the probe surface, followed by chemical ionization, and not by surface ionization.", "contents": "The effects of heating rate and sample size on the direct exposure/chemical ionization mass spectra of some biological conjugates. A direct exposure probe has been used to obtain mass spectra of underivatized guanosine, deoxyguanosine, sucrose and the p-nitrophenyl-beta-D-glucuronide. In all cases, a protonated molecular ion is produced with good relative abundance. The effects of heating rate and sample size on the production of the [MH]+ ION ARE EXAMINED IN DETAIL FROM TOTAL ION ANd single ion currents produced during rapid, repetitive scanning of the spectra after probe insertion. From this data we conclude that protonated molecular ions are produced as a result of the enhanced volatility of neutral molecules on the probe surface, followed by chemical ionization, and not by surface ionization."}
{"id": "PMID:486715", "title": "The identification of 3-keto-2-methylvaleric acid and 3-hydroxy-2-methylvaleric acid in a patient with propionic acidemia.", "content": "Two abnormal metabolites, 3-keto-2-methylvaleric acid and 3-hydroxy-2-methylvaleric acid, have been identified and quantitated in the urine of a child with propionic acidemia. These metabolites may be produced as a result of the self-condensation of propionyl-CoA. Data are presented to show that the unusual ketone, 3-pentanone, which has been observed previously in the urine of patients with propionic acidemia, is produced as a result of the decarboxylation of 3-keto-2-methylvaleric acid.", "contents": "The identification of 3-keto-2-methylvaleric acid and 3-hydroxy-2-methylvaleric acid in a patient with propionic acidemia. Two abnormal metabolites, 3-keto-2-methylvaleric acid and 3-hydroxy-2-methylvaleric acid, have been identified and quantitated in the urine of a child with propionic acidemia. These metabolites may be produced as a result of the self-condensation of propionyl-CoA. Data are presented to show that the unusual ketone, 3-pentanone, which has been observed previously in the urine of patients with propionic acidemia, is produced as a result of the decarboxylation of 3-keto-2-methylvaleric acid."}
{"id": "PMID:486716", "title": "Synthesis and back exchange of 18O labeled amino acids for use as internal standards with mass spectrometry.", "content": "Amino acids with two oxygen-18 atoms in the carboxyl moiety can be synthesized easily from the amino acids and H2 18O through exchange. The exchange conditions are simply solution of the amino acid (1-4 mg) in acidic H2 18O (0.1-0.2ml) followed by heating at 60-70 degrees C for several days. Eighteen oxygen-18 amino acids with incorporations typically greater than 90% 18O2 have been achieved and characterized by mass spectrometry. The exchange conditions do not lead to racemization. Loss of the oxygen-18 atoms through back exchange was found to be pH and temperature dependent, but yet slow enough to allow these isotopimers to be used in most studies as internal standards for quantitative mass spectrometry. The 18O carboxylic atoms were not exchanged upon incubation in plasma for three days at 37 degrees C, PH 7.4. The exchange reaction is general enough to allow the simultaneous synthesis of several [18O] amino acids.", "contents": "Synthesis and back exchange of 18O labeled amino acids for use as internal standards with mass spectrometry. Amino acids with two oxygen-18 atoms in the carboxyl moiety can be synthesized easily from the amino acids and H2 18O through exchange. The exchange conditions are simply solution of the amino acid (1-4 mg) in acidic H2 18O (0.1-0.2ml) followed by heating at 60-70 degrees C for several days. Eighteen oxygen-18 amino acids with incorporations typically greater than 90% 18O2 have been achieved and characterized by mass spectrometry. The exchange conditions do not lead to racemization. Loss of the oxygen-18 atoms through back exchange was found to be pH and temperature dependent, but yet slow enough to allow these isotopimers to be used in most studies as internal standards for quantitative mass spectrometry. The 18O carboxylic atoms were not exchanged upon incubation in plasma for three days at 37 degrees C, PH 7.4. The exchange reaction is general enough to allow the simultaneous synthesis of several [18O] amino acids."}
{"id": "PMID:486717", "title": "Mass-transfer effects on the rate of isomerization of D-glucose into D-fructose, catalyzed by whole-cell immobilized glucose isomerase.", "content": "The investigated catalyst system consists of immobilized Arthrobacter cells containing the enzyme glucose isomerase, which catalyzes the isomerization of glucose into fructose. The internal structure of the catalyst was determined from electrom microscope photographs of replicas of freeze-etched catalyst. On the basis of the photographs a model for the internal structure of the catalyst was proposed. This structure was subsequently used to describe the reaction including mass-transfer effects. It appeared that under normal operating conditions the external mass-transfer rate does not influence the overall rate of reaction. The effect of internal mass-transfer resistances on the overall reaction rate can well be accounted for by the so-called porous sphere model. The intrinsic kinetics of the isomerization catalyzed by the present catalyst system can be represented by a modified Michaelis-Menten equation for a reversible one-substrate reaction.", "contents": "Mass-transfer effects on the rate of isomerization of D-glucose into D-fructose, catalyzed by whole-cell immobilized glucose isomerase. The investigated catalyst system consists of immobilized Arthrobacter cells containing the enzyme glucose isomerase, which catalyzes the isomerization of glucose into fructose. The internal structure of the catalyst was determined from electrom microscope photographs of replicas of freeze-etched catalyst. On the basis of the photographs a model for the internal structure of the catalyst was proposed. This structure was subsequently used to describe the reaction including mass-transfer effects. It appeared that under normal operating conditions the external mass-transfer rate does not influence the overall rate of reaction. The effect of internal mass-transfer resistances on the overall reaction rate can well be accounted for by the so-called porous sphere model. The intrinsic kinetics of the isomerization catalyzed by the present catalyst system can be represented by a modified Michaelis-Menten equation for a reversible one-substrate reaction."}
{"id": "PMID:486718", "title": "Kinetics and stability of immobilized chicken liver xanthine dehydrogenase.", "content": "Xanthine dehydrogenase (EC 1.2.1.37) was isolated from chicken livers and immobilized by adsorption to a Sepharose derivative, prepared by reaction of n-octylamine with CNBr-activated Sepharose 4B. Using a crude preparation of enzyme for immobilization it was observed that relatively more activity was adsorbed than protein, but the yield of immobilized activity increased as a purer enzyme preparation was used. As more activity and protein were bound, relatively less immobilized activity was recovered. This effect was probably due to blocking of active xanthine dehydrogenase by protein impurities. The kinetics of free and immobilized xanthine dehydrogenase were studied in the pH range 7.5-9.1. The Km and V values estimated for free xanthine dehydrogenase increase as the pH increase; the K'm and V values for the immobilized enzyme go through a minimum at pH 8.1. By varying the amount of enzyme activity bound per unit volume of gel, it was shown that K'm is larger than Km are result of substrate diffusion limitation in the pores of the support material. Both free and immobilized xanthine dehydrogenase showed substrate activation at low concentrations (up to 2 microM xanthine). Immobilized xanthine dehydrogenase was more stable than the free enzyme during storage in the temperature range of 4-50 degrees C. The operational stability of immobilized xanthine dehydrogenase at 30 degrees C was two orders of magnitude smaller than the storage stability, t 1/2 was 9 and 800 hr, respectively. The operational stability was, however, better than than of immobilized milk xanthine oxidase (t 1/2 = 1 hr). In addition, the amount of product formed per unit initial activity in one half-life, was higher for immobilized xanthine dehydrogenase than for immobilized xanthine oxidase. Unless immobilized milk xanthine oxidase can be considerable stabilized, immobilized chicken liver xanthine dehydrogenase is more promising for application in organic synthesis.", "contents": "Kinetics and stability of immobilized chicken liver xanthine dehydrogenase. Xanthine dehydrogenase (EC 1.2.1.37) was isolated from chicken livers and immobilized by adsorption to a Sepharose derivative, prepared by reaction of n-octylamine with CNBr-activated Sepharose 4B. Using a crude preparation of enzyme for immobilization it was observed that relatively more activity was adsorbed than protein, but the yield of immobilized activity increased as a purer enzyme preparation was used. As more activity and protein were bound, relatively less immobilized activity was recovered. This effect was probably due to blocking of active xanthine dehydrogenase by protein impurities. The kinetics of free and immobilized xanthine dehydrogenase were studied in the pH range 7.5-9.1. The Km and V values estimated for free xanthine dehydrogenase increase as the pH increase; the K'm and V values for the immobilized enzyme go through a minimum at pH 8.1. By varying the amount of enzyme activity bound per unit volume of gel, it was shown that K'm is larger than Km are result of substrate diffusion limitation in the pores of the support material. Both free and immobilized xanthine dehydrogenase showed substrate activation at low concentrations (up to 2 microM xanthine). Immobilized xanthine dehydrogenase was more stable than the free enzyme during storage in the temperature range of 4-50 degrees C. The operational stability of immobilized xanthine dehydrogenase at 30 degrees C was two orders of magnitude smaller than the storage stability, t 1/2 was 9 and 800 hr, respectively. The operational stability was, however, better than than of immobilized milk xanthine oxidase (t 1/2 = 1 hr). In addition, the amount of product formed per unit initial activity in one half-life, was higher for immobilized xanthine dehydrogenase than for immobilized xanthine oxidase. Unless immobilized milk xanthine oxidase can be considerable stabilized, immobilized chicken liver xanthine dehydrogenase is more promising for application in organic synthesis."}
{"id": "PMID:486720", "title": "Analysis of the coupled transport, reaction, and deactivation phenomena in the immobilized glucose oxidase and catalase system.", "content": "In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kinetics. Furthermore, the deactivation of both enzymes due to the intermediate product peroxide is taken into account. The predicted results suggest that the efficiency of the glucose oxidase reaction depends on the concentration ranges of the two substrates. Furthermore, the external mass-transfer rate may cause a shift from glucose limitation to oxygen limitation. The efficiency of the coupled system is always higher than that predicted for the uncoupled reaction path. The calculations show that the economics of the coupled system depend a great deal on the deactivation of the enzymes.", "contents": "Analysis of the coupled transport, reaction, and deactivation phenomena in the immobilized glucose oxidase and catalase system. In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kinetics. Furthermore, the deactivation of both enzymes due to the intermediate product peroxide is taken into account. The predicted results suggest that the efficiency of the glucose oxidase reaction depends on the concentration ranges of the two substrates. Furthermore, the external mass-transfer rate may cause a shift from glucose limitation to oxygen limitation. The efficiency of the coupled system is always higher than that predicted for the uncoupled reaction path. The calculations show that the economics of the coupled system depend a great deal on the deactivation of the enzymes."}
{"id": "PMID:486721", "title": "Hydrophobic esters of cellulose: properties and applications in biochemical technology.", "content": "We have investigated the utility for enzyme immobilization of several hydrophobic cellulose esters, as a function of solvent composition, extent of esterification, and enzyme. Phenoxyacetyl cellulose was also used for immobilization of rat liver microsomes, hydrophobic chromatography of proteins, and removal of Triton X-100 from protein solutions. Phenoxyacetyl groups esterified to cellulose were much less subject to enzymatic hydrolysis than soluble phenoxyacetyl esters.", "contents": "Hydrophobic esters of cellulose: properties and applications in biochemical technology. We have investigated the utility for enzyme immobilization of several hydrophobic cellulose esters, as a function of solvent composition, extent of esterification, and enzyme. Phenoxyacetyl cellulose was also used for immobilization of rat liver microsomes, hydrophobic chromatography of proteins, and removal of Triton X-100 from protein solutions. Phenoxyacetyl groups esterified to cellulose were much less subject to enzymatic hydrolysis than soluble phenoxyacetyl esters."}
{"id": "PMID:486722", "title": "An electrochemical sensor for continuous intravascular oxygen monitoring.", "content": "The intravascular PO2 sensor described in this paper essentialal routine use. The small dimensions of the sensor permit its introduction into the vessel through a conventional guiding catheter size 4 F or larger. With 5 F catheters, simultaneous blood sampling and/or blood pressure measurement is possible. The accuracy and stability of the sensor permit continuous PO2 measurement to be performed over several days.", "contents": "An electrochemical sensor for continuous intravascular oxygen monitoring. The intravascular PO2 sensor described in this paper essentialal routine use. The small dimensions of the sensor permit its introduction into the vessel through a conventional guiding catheter size 4 F or larger. With 5 F catheters, simultaneous blood sampling and/or blood pressure measurement is possible. The accuracy and stability of the sensor permit continuous PO2 measurement to be performed over several days."}
{"id": "PMID:486723", "title": "Intraarterial vs. transcutaneous PO2 monitoring in newborn infants. Indications and limitations.", "content": "For continuous PO2 monitoring as a valuable diagnostic tool in intensive care of newborn infants two methods are available, namely transcutaneous and intravascular monitoring. Both methods have advantages and limitations, but they are rather complementary than competing. The necessity of these new methods and the clinical implications are described.", "contents": "Intraarterial vs. transcutaneous PO2 monitoring in newborn infants. Indications and limitations. For continuous PO2 monitoring as a valuable diagnostic tool in intensive care of newborn infants two methods are available, namely transcutaneous and intravascular monitoring. Both methods have advantages and limitations, but they are rather complementary than competing. The necessity of these new methods and the clinical implications are described."}
{"id": "PMID:486724", "title": "Practical experiences with the routine application of the intravascular PO2 probe.", "content": "The practical experience with the routine use of 53 Roche PO2 probes, introduced through the radial artery in artificially ventilated patients of all age groups, during up to 8 1/2 days, is described. It may be concluded that many observations are only possible by continuous measurement, which, for example, makes possible a quicker and more accurate control of breathing and respiration. In some cases the cost-benefit calculation even favors continuous measurement.", "contents": "Practical experiences with the routine application of the intravascular PO2 probe. The practical experience with the routine use of 53 Roche PO2 probes, introduced through the radial artery in artificially ventilated patients of all age groups, during up to 8 1/2 days, is described. It may be concluded that many observations are only possible by continuous measurement, which, for example, makes possible a quicker and more accurate control of breathing and respiration. In some cases the cost-benefit calculation even favors continuous measurement."}
{"id": "PMID:486725", "title": "Continuous measurement of arterial PO2. Significance and indications in intensive care.", "content": "The use of continuous arterial PO2 monitoring with the aid of intra-arterial electrodes is discussed with reference to 50 patients in an intensive care unit specializing in internal medicine. Monitoring time ranged from 2 h to 15 days. There were no serious complications which could, with certainty, be attributed to the intravascular measurement of PO2. The advantages of this method lie particularly in the early detection of hypoxemias, in the uninterrupted monitoring of artificial respiration and in the immediate indication of favorable and unfavorable response to therapy. Finally, a list is given of possible indications in which this method can be used in intensive medicine.", "contents": "Continuous measurement of arterial PO2. Significance and indications in intensive care. The use of continuous arterial PO2 monitoring with the aid of intra-arterial electrodes is discussed with reference to 50 patients in an intensive care unit specializing in internal medicine. Monitoring time ranged from 2 h to 15 days. There were no serious complications which could, with certainty, be attributed to the intravascular measurement of PO2. The advantages of this method lie particularly in the early detection of hypoxemias, in the uninterrupted monitoring of artificial respiration and in the immediate indication of favorable and unfavorable response to therapy. Finally, a list is given of possible indications in which this method can be used in intensive medicine."}
{"id": "PMID:486727", "title": "Clinical suitability and accuracy of a new combined system for the transcutaneous and intravascular determination of PO2.", "content": "The clinical suitability and accuracy of the Hoffmann-La Roche continuous oxygen partial pressure measuring system, consisting of transcutaneous and intravascular probes, modules and recorder, was tested in routine use in a unit for vascular and heart surgery of the University Clinic in G\u00f6ttingen. The stability of the system was good, accuracy was sufficient for clinical purposes (compared with a conventional discrete blood gas analysis technique) and practical suitability was good in routine use. The unit was simple to use, provided preparatory and calibratory steps were completed with necessary care and precision. Based on our experience with the unit, it is correct to say that it represents a refined and sufficiently accurate method suitable for use under clinical conditions. The need for strict adherence to the operating instructions must, however, be emphasized if incorrect measurements are to be avoided.", "contents": "Clinical suitability and accuracy of a new combined system for the transcutaneous and intravascular determination of PO2. The clinical suitability and accuracy of the Hoffmann-La Roche continuous oxygen partial pressure measuring system, consisting of transcutaneous and intravascular probes, modules and recorder, was tested in routine use in a unit for vascular and heart surgery of the University Clinic in G\u00f6ttingen. The stability of the system was good, accuracy was sufficient for clinical purposes (compared with a conventional discrete blood gas analysis technique) and practical suitability was good in routine use. The unit was simple to use, provided preparatory and calibratory steps were completed with necessary care and precision. Based on our experience with the unit, it is correct to say that it represents a refined and sufficiently accurate method suitable for use under clinical conditions. The need for strict adherence to the operating instructions must, however, be emphasized if incorrect measurements are to be avoided."}
{"id": "PMID:486730", "title": "[Treatment of rhabdomyosarcoma in mice C3H/He by Co 60 and hyperbaric oxygen (author's transl)].", "content": "Experimental study of rhabdomyosarcoma with successive transplantation upon C3H/He mice, treated by irradiation (Co 60) and combined irradiation-hyperbaric oxygen (HBO), dating from 3, 14 and 15 days after transplantation. The data (tumor volume evolution, histological modifications, pulmonary metastases) are compared with controls. curative radiotherapy depends on starting treatment as soon as possible with or without HBO. After the 14th day, sensitisation to combined HBO and C60 is seen. The extension of pulmonary metastases is a function of tumor growth. Paradoxically metastases were less frequent after HBO only and more frequent after HBO-Co 60.", "contents": "[Treatment of rhabdomyosarcoma in mice C3H/He by Co 60 and hyperbaric oxygen (author's transl)]. Experimental study of rhabdomyosarcoma with successive transplantation upon C3H/He mice, treated by irradiation (Co 60) and combined irradiation-hyperbaric oxygen (HBO), dating from 3, 14 and 15 days after transplantation. The data (tumor volume evolution, histological modifications, pulmonary metastases) are compared with controls. curative radiotherapy depends on starting treatment as soon as possible with or without HBO. After the 14th day, sensitisation to combined HBO and C60 is seen. The extension of pulmonary metastases is a function of tumor growth. Paradoxically metastases were less frequent after HBO only and more frequent after HBO-Co 60."}
{"id": "PMID:486731", "title": "Distribution and consequences of cell suspensions following intralymphatic infusion.", "content": "The fate and consequences of intralymphatic injections of cells was investigated in dogs. The distribution of intact radiolabeled cells was determined in vivo by whole body gamma scanning. Comparison of distributions resulting from intralymphatic, subcutaneous, intradermal and intravenous routes of administration showed that the distribution and duration of radiolabel in various organs varied with the route of administration. Following intralymphatic injection, radiolabel was concentrated in first echelon lymph nodes draining the site of injection and was retained in these nodes for over 4 weeks. Histologic studies showed intense cortical and paracortical lymphopoiesis to be associated with the retention of intralymphatically injected tumor cells by first echelon lymph nodes. Serial histologic examination of lymph nodes from intralymphatically injected inbred beagles revealed that the consequent lymphopoiesis persisted for 5 weeks. In vitro evaluation of peripheral blood and lymph node lymphocyte cytotoxicity to the injected cells indicated that retention and nodal lymphopoiesis was associated with the development of direct lymphocyte cytotoxicity. The effects of concommitant tumor burden, cytotoxic drugs and ionizing radiation were also investigated and suggest that the therapeutic potential for use of the intralymphatic route has not yet been realized.", "contents": "Distribution and consequences of cell suspensions following intralymphatic infusion. The fate and consequences of intralymphatic injections of cells was investigated in dogs. The distribution of intact radiolabeled cells was determined in vivo by whole body gamma scanning. Comparison of distributions resulting from intralymphatic, subcutaneous, intradermal and intravenous routes of administration showed that the distribution and duration of radiolabel in various organs varied with the route of administration. Following intralymphatic injection, radiolabel was concentrated in first echelon lymph nodes draining the site of injection and was retained in these nodes for over 4 weeks. Histologic studies showed intense cortical and paracortical lymphopoiesis to be associated with the retention of intralymphatically injected tumor cells by first echelon lymph nodes. Serial histologic examination of lymph nodes from intralymphatically injected inbred beagles revealed that the consequent lymphopoiesis persisted for 5 weeks. In vitro evaluation of peripheral blood and lymph node lymphocyte cytotoxicity to the injected cells indicated that retention and nodal lymphopoiesis was associated with the development of direct lymphocyte cytotoxicity. The effects of concommitant tumor burden, cytotoxic drugs and ionizing radiation were also investigated and suggest that the therapeutic potential for use of the intralymphatic route has not yet been realized."}
{"id": "PMID:486732", "title": "[CEA and breast neoplasms: application of a personal method for determination of CEA in the clinical study of 242 patients (author's transl)].", "content": "The authors report their experience of the clinical application of monitoring serum CEA levels in breast tumours, obtained by a new direct method of determination. A total of 242 patients were studied: 124 with benign tumours, 75 with primary carcinoma in various stages; and 43 who had previously undergone to surgery for breast carcinoma. The analysis of pre-surgical specimens demonstrate consistently normal values for the benign tumors, whereas patients with malignant tumours had positive values in 40 p. 100 of cases. Significant differences in values were seen between benign tumours and Stage II malignant tumours, and between Stage II and Stage III tumours, whereas there was no significant difference between benign forms and Stage I, and between Stage III and Stage IV. In patients with metastases, followed up after surgery, the CEA was positive in 94 p. 100 of the cases. In the opinion of the authors, CEA levels do not seem to be useful for the early diagnosis of malignant breast tumours but are important, harmless and inexpensive, in the follow-up of patients who have undergone surgery for breast neoplasms.", "contents": "[CEA and breast neoplasms: application of a personal method for determination of CEA in the clinical study of 242 patients (author's transl)]. The authors report their experience of the clinical application of monitoring serum CEA levels in breast tumours, obtained by a new direct method of determination. A total of 242 patients were studied: 124 with benign tumours, 75 with primary carcinoma in various stages; and 43 who had previously undergone to surgery for breast carcinoma. The analysis of pre-surgical specimens demonstrate consistently normal values for the benign tumors, whereas patients with malignant tumours had positive values in 40 p. 100 of cases. Significant differences in values were seen between benign tumours and Stage II malignant tumours, and between Stage II and Stage III tumours, whereas there was no significant difference between benign forms and Stage I, and between Stage III and Stage IV. In patients with metastases, followed up after surgery, the CEA was positive in 94 p. 100 of the cases. In the opinion of the authors, CEA levels do not seem to be useful for the early diagnosis of malignant breast tumours but are important, harmless and inexpensive, in the follow-up of patients who have undergone surgery for breast neoplasms."}
{"id": "PMID:486733", "title": "[Thyroid trabecular carcinoma. A clinicopathological entity of poor prognosis? (author's transl)].", "content": "The records of 32 patients with trabecular carcinomas of the thyroid gland were critically reviewed from a previously published serie of 138 thyroid cancers referred to the Centre Claudius Regaud, between 1952 and 1973. On the basis of clinico-pathological considerations, it seems possible to divide trabecular carcinomas into two groups. Pure trabecular carcinomas (moderately differenciated follicular carcinomas--WOH) which have a poor prognosis (5 years actuarial survival: 13%) related to high rate of local recurrences, fast metastatic spread to the lung, bad response to suppressive hormonotherapy and lack of 131 iode uptake by malignant tissue. Mixed trabeculo-vesicular carcinomas which have in comparison a fairly good prognosis (5 years actuarial survival: 63%) in keeping with a lower tendency to local recurrences and a useful concentration of radioactive iodine by metastases (most of them located in the skeleton) although dependent on the pourcentage of vesicles in the tumor process. Among differentiated thyroid carcinomas, distinction between pure trabecular and mixed trabeculo-vesicular carcinomas with quantitative determination of vesicules seems of great interest in relation to the therapeutic approach.", "contents": "[Thyroid trabecular carcinoma. A clinicopathological entity of poor prognosis? (author's transl)]. The records of 32 patients with trabecular carcinomas of the thyroid gland were critically reviewed from a previously published serie of 138 thyroid cancers referred to the Centre Claudius Regaud, between 1952 and 1973. On the basis of clinico-pathological considerations, it seems possible to divide trabecular carcinomas into two groups. Pure trabecular carcinomas (moderately differenciated follicular carcinomas--WOH) which have a poor prognosis (5 years actuarial survival: 13%) related to high rate of local recurrences, fast metastatic spread to the lung, bad response to suppressive hormonotherapy and lack of 131 iode uptake by malignant tissue. Mixed trabeculo-vesicular carcinomas which have in comparison a fairly good prognosis (5 years actuarial survival: 63%) in keeping with a lower tendency to local recurrences and a useful concentration of radioactive iodine by metastases (most of them located in the skeleton) although dependent on the pourcentage of vesicles in the tumor process. Among differentiated thyroid carcinomas, distinction between pure trabecular and mixed trabeculo-vesicular carcinomas with quantitative determination of vesicules seems of great interest in relation to the therapeutic approach."}
{"id": "PMID:486735", "title": "[Mortality of bladder cancer in France: 1952--1976. Trends and increase, particularly in the South of France (author's transl)].", "content": "Trends in mortality from bladder cancer have been described with several statistical methods using bi and multivariate analysis. Between 1952 and 1976, the mortality by bladder cancer was marked by an increase for both sexes with a predominance among the males (annual increase + 3,65%, against 1.34% among the females). In 1976, the age-adjusted rate/100,000 of mortality was 11,35 for males and 3,20 for the females showing an high sex-ratio. The computerized mapping of this cancer geographical distribution shows a significant predominance in the south of France and also a high statistically significant correlation with the geographical distribution of lung and gallbladder cancer mortality in France.", "contents": "[Mortality of bladder cancer in France: 1952--1976. Trends and increase, particularly in the South of France (author's transl)]. Trends in mortality from bladder cancer have been described with several statistical methods using bi and multivariate analysis. Between 1952 and 1976, the mortality by bladder cancer was marked by an increase for both sexes with a predominance among the males (annual increase + 3,65%, against 1.34% among the females). In 1976, the age-adjusted rate/100,000 of mortality was 11,35 for males and 3,20 for the females showing an high sex-ratio. The computerized mapping of this cancer geographical distribution shows a significant predominance in the south of France and also a high statistically significant correlation with the geographical distribution of lung and gallbladder cancer mortality in France."}
{"id": "PMID:486774", "title": "Influence of Temik (aldicarb) on herbicide persistence in cultivated cotton field soil under field conditions.", "content": "The effect of the insecticide Temik on the persistence of the herbicides Cotoran and Cobex was investigated in cultivated cotton fields. Temik was applied to the soil which had also been treated with Cotoran or Cobex. Residues were determined at 15 day intervals for four months. Temik was found to inhibit the degradation of both compounds as long as it persisted. As Temik disappeared the degradtation of Cotoran and Cobex increased, attaining after the 15th day the rates shown by Cotoran and Cobex when applied in isolation.", "contents": "Influence of Temik (aldicarb) on herbicide persistence in cultivated cotton field soil under field conditions. The effect of the insecticide Temik on the persistence of the herbicides Cotoran and Cobex was investigated in cultivated cotton fields. Temik was applied to the soil which had also been treated with Cotoran or Cobex. Residues were determined at 15 day intervals for four months. Temik was found to inhibit the degradation of both compounds as long as it persisted. As Temik disappeared the degradtation of Cotoran and Cobex increased, attaining after the 15th day the rates shown by Cotoran and Cobex when applied in isolation."}
{"id": "PMID:486789", "title": "Compartmentalization of 14C-DDT in an experimental old-field plot.", "content": "Of the 2.81 X 10(6) micrograms DDT applied to the plot in June 1976, 2.58 X 10(6) micrograms (91.8%) were accounted for as of June 1977. Silk screens, ethylene glycol air samples, and run-off contained 40.57 X 40(-3)%, 0.09 X 10(-3)%, and 1.18 X 10(-3)%, respectively of the DDT aplied. Soil was the major sink for DDT in this study, with 91.8% of the applied DDT located there. Most of the remaining 8.2% was probably lost during the formulation and application of the 14C-DDT granules, some was probably lost in organisms (mainly flying insects) leaving the plot, and some was accounted for by experimental error and error in the counting technique.", "contents": "Compartmentalization of 14C-DDT in an experimental old-field plot. Of the 2.81 X 10(6) micrograms DDT applied to the plot in June 1976, 2.58 X 10(6) micrograms (91.8%) were accounted for as of June 1977. Silk screens, ethylene glycol air samples, and run-off contained 40.57 X 40(-3)%, 0.09 X 10(-3)%, and 1.18 X 10(-3)%, respectively of the DDT aplied. Soil was the major sink for DDT in this study, with 91.8% of the applied DDT located there. Most of the remaining 8.2% was probably lost during the formulation and application of the 14C-DDT granules, some was probably lost in organisms (mainly flying insects) leaving the plot, and some was accounted for by experimental error and error in the counting technique."}
{"id": "PMID:486809", "title": "Earphone termination and the response of behind-the-ear hearing aids.", "content": "An investigation was carried out on the effects on hearing aid response of placing the earphone in the earmould rather than in the case of a behind-the-ear hearing aid. Results from a number of physical configurations are reported and discussed and it is shown that it is possible to achieve a considerable reduction in the height of frequency response peaks. On the basis of the research reported some specific recommendations are made about future aid design.", "contents": "Earphone termination and the response of behind-the-ear hearing aids. An investigation was carried out on the effects on hearing aid response of placing the earphone in the earmould rather than in the case of a behind-the-ear hearing aid. Results from a number of physical configurations are reported and discussed and it is shown that it is possible to achieve a considerable reduction in the height of frequency response peaks. On the basis of the research reported some specific recommendations are made about future aid design."}
{"id": "PMID:486810", "title": "Investigation of the relationship between adaptation measured binaurally and monaurally.", "content": "Recently it has been demonstrated that auditory adaptation can be measured with a monaural technique (Weiler and Friedman, 1973; Weiler and Gross, 1976; Feaster and Weiler, 1975). Because the magnitude of adaptation measured monaurally was nearly identical to that measured binaurally in other experiments, it was assumed that they measured the same phenomenon or phenomena. In the present study the same group of subjects were tested for magnitude of adaptation both monaurally and binaurally. The means were 11.75 dB and 12.17 dB, respectively (N=13). Depsite nearly identical means for the present conditions there was no significant correlation (r=+.27) between the magnitude of adaptation measured under the two conditions. Except for the possibility that individual differences in the ability to do monaural versus binaural tasks may have obscured individual similarities in adaptation, the only other explanation the authors can suggest is that the two techniques measure different aspects of loudness adaptation.", "contents": "Investigation of the relationship between adaptation measured binaurally and monaurally. Recently it has been demonstrated that auditory adaptation can be measured with a monaural technique (Weiler and Friedman, 1973; Weiler and Gross, 1976; Feaster and Weiler, 1975). Because the magnitude of adaptation measured monaurally was nearly identical to that measured binaurally in other experiments, it was assumed that they measured the same phenomenon or phenomena. In the present study the same group of subjects were tested for magnitude of adaptation both monaurally and binaurally. The means were 11.75 dB and 12.17 dB, respectively (N=13). Depsite nearly identical means for the present conditions there was no significant correlation (r=+.27) between the magnitude of adaptation measured under the two conditions. Except for the possibility that individual differences in the ability to do monaural versus binaural tasks may have obscured individual similarities in adaptation, the only other explanation the authors can suggest is that the two techniques measure different aspects of loudness adaptation."}
{"id": "PMID:486811", "title": "Auditory deprivation--an intrinsic or extrinsic problem? Some comments on Kyle (1978).", "content": "The relevance of animal work to early auditory deprivation, as discussed by Kyle (1978), is questioned. It is argued that auditory experience from birth, or even much later, is not necessarily required for subsequent hearing for spoken language. It is also questioned whether concern for intrinsic difficulties, such as possible cortical damage resulting from auditory deprivation, is appropriate. A more productive approach may be to pay more attention to the extrinsic aspect--the linguistically principled rehabilitation of the hearing-impaired child.", "contents": "Auditory deprivation--an intrinsic or extrinsic problem? Some comments on Kyle (1978). The relevance of animal work to early auditory deprivation, as discussed by Kyle (1978), is questioned. It is argued that auditory experience from birth, or even much later, is not necessarily required for subsequent hearing for spoken language. It is also questioned whether concern for intrinsic difficulties, such as possible cortical damage resulting from auditory deprivation, is appropriate. A more productive approach may be to pay more attention to the extrinsic aspect--the linguistically principled rehabilitation of the hearing-impaired child."}
{"id": "PMID:486812", "title": "Air-borne radiation from bone conduction transducers.", "content": "The relative magnitudes of air-borne radiation and mechanical vibration from three types of bone conduction transducer have been measured. The study suggests that the presence of excess air-borne radiation can lead to the observation of a false air-bone gap in audiometry. Some methods of overcoming this effect are considered.", "contents": "Air-borne radiation from bone conduction transducers. The relative magnitudes of air-borne radiation and mechanical vibration from three types of bone conduction transducer have been measured. The study suggests that the presence of excess air-borne radiation can lead to the observation of a false air-bone gap in audiometry. Some methods of overcoming this effect are considered."}
{"id": "PMID:486813", "title": "Monaural balances and loudness coding during auditory adaptation.", "content": "A monaural simultaneous balance technique was used to compare amounts of adaptation for two conditions: (1) with the test intensity equal to the adapting intensity, and (2) with the test intensity +10 dB above the adapting intensity. Previous findings (Weiler and Hood, 1977) showed a decline in measured adaptation for the second condition using simultaneous binaural balances. That this was not the case for the monaural balances investigated in this study may indicate different adaptation-induced auditory changes are being measured monaurally vs. binaurally.", "contents": "Monaural balances and loudness coding during auditory adaptation. A monaural simultaneous balance technique was used to compare amounts of adaptation for two conditions: (1) with the test intensity equal to the adapting intensity, and (2) with the test intensity +10 dB above the adapting intensity. Previous findings (Weiler and Hood, 1977) showed a decline in measured adaptation for the second condition using simultaneous binaural balances. That this was not the case for the monaural balances investigated in this study may indicate different adaptation-induced auditory changes are being measured monaurally vs. binaurally."}
{"id": "PMID:486816", "title": "The BKB (Bamford-Kowal-Bench) sentence lists for partially-hearing children.", "content": "Linguistic guidelines for the design of sentences for speech audiometry with children are described, and new lists of test sentences which are based on such guidelines--the Bamford-Kowal-Bench Sentence Lists for Children--are introduced. Audiometric data relating to the use of the new lists are presented and discussed.", "contents": "The BKB (Bamford-Kowal-Bench) sentence lists for partially-hearing children. Linguistic guidelines for the design of sentences for speech audiometry with children are described, and new lists of test sentences which are based on such guidelines--the Bamford-Kowal-Bench Sentence Lists for Children--are introduced. Audiometric data relating to the use of the new lists are presented and discussed."}
{"id": "PMID:486817", "title": "The effect of content of initial instructions on the speech discrimination scores of hearing and hearing-impaired children.", "content": "This paper investigated the effects of content of initial instructions on the speech discrimination abilities of 8 groups of children (4 groups of hearing children; 4 groups of hearing-impaired children--N = 10 children in each group). The speech discrimination abilities of the children in each group were ascertained with the AB monosyllabic word lists following two modes of initial instructions. It was concluded that with monosyllabic word lists scored phonemically it is essential to instruct each listener to repeat not meaningful whole words but also everything else that he/she recognises, including words which do not make sense, or parts of the words or even single sounds. Failure to highlight in the initial instructions the phonemic aspects of scoring brings about significantly lower speech discrimination scores.", "contents": "The effect of content of initial instructions on the speech discrimination scores of hearing and hearing-impaired children. This paper investigated the effects of content of initial instructions on the speech discrimination abilities of 8 groups of children (4 groups of hearing children; 4 groups of hearing-impaired children--N = 10 children in each group). The speech discrimination abilities of the children in each group were ascertained with the AB monosyllabic word lists following two modes of initial instructions. It was concluded that with monosyllabic word lists scored phonemically it is essential to instruct each listener to repeat not meaningful whole words but also everything else that he/she recognises, including words which do not make sense, or parts of the words or even single sounds. Failure to highlight in the initial instructions the phonemic aspects of scoring brings about significantly lower speech discrimination scores."}
{"id": "PMID:486818", "title": "Effects of jet noise on mortality rates.", "content": "Two areas, containing a total of over 160,000 people, were examined for mortality rates; one area was directly under incoming flights, near Los Angeles International Airport (LAX). The other was removed from the LAX flight patterns so that jet noise was not dominant. The two areas were chosen so that they were as nearly alike as possible in age, racial distribution, income and in other relevant factors with the sole major difference of jet noise in one of them. It was found that there was a substantial increase in mortality rates in the area under the jets where there was large noise radiation. In particular, by a most conservative statistical treatment there was in the jet noise area: a 15% increase in deaths due to strokes (cerebro-vascular disease) which accounted for 39 deaths in the two-year period of the study--presumably attributable to the excessive jet noise. Further, in the noise-radiated area there was a 100% increase in deaths due to cirrhosis of the liver (primarily attributed to alcoholism)--amounting to 24 extra deaths in the two years, due to jet noise. One of the disturbing side results of this study was that in these relatively poor regions it appears that there should be about 50% more deaths than were reported and recorded by Los Angeles County. These losses were perhaps due to a concentration of bad, given addresses in the areas in question; this serious loss casts grave doubt on previous studies of eath rates for minority peoples (e.g. blacks), suggesting that the rates may be considerably higher than those previously reported.", "contents": "Effects of jet noise on mortality rates. Two areas, containing a total of over 160,000 people, were examined for mortality rates; one area was directly under incoming flights, near Los Angeles International Airport (LAX). The other was removed from the LAX flight patterns so that jet noise was not dominant. The two areas were chosen so that they were as nearly alike as possible in age, racial distribution, income and in other relevant factors with the sole major difference of jet noise in one of them. It was found that there was a substantial increase in mortality rates in the area under the jets where there was large noise radiation. In particular, by a most conservative statistical treatment there was in the jet noise area: a 15% increase in deaths due to strokes (cerebro-vascular disease) which accounted for 39 deaths in the two-year period of the study--presumably attributable to the excessive jet noise. Further, in the noise-radiated area there was a 100% increase in deaths due to cirrhosis of the liver (primarily attributed to alcoholism)--amounting to 24 extra deaths in the two years, due to jet noise. One of the disturbing side results of this study was that in these relatively poor regions it appears that there should be about 50% more deaths than were reported and recorded by Los Angeles County. These losses were perhaps due to a concentration of bad, given addresses in the areas in question; this serious loss casts grave doubt on previous studies of eath rates for minority peoples (e.g. blacks), suggesting that the rates may be considerably higher than those previously reported."}
{"id": "PMID:486819", "title": "Hearing aid candidates--some relevant features.", "content": "The age of individual seeking NHS aids has risen steadily since the service was commenced, and the median age is now over 70 years. The major type of hearing loss observed is sensorineural, but a substantial proportion of hearing aid candidates still have a significant conductive element in their hearing loss. This latter group of patients tend to be significantly younger than those without middle ear involvement. The hearing loss when applying for a hearing aid tends to be around 50 dB but is age dependent, younger subjects coming with smaller hearing losses. The most disturbing information obtained on questioning applicants was the long time lapse between recognition of hearing impairment and application for a hearing aid. This averaged almost ten years. The true delay is probably considerably greater than this and must have a substantial bearing on the outcome of hearing aid fitting. Public education and counselling of hearing aid users are suggested as vita needs.", "contents": "Hearing aid candidates--some relevant features. The age of individual seeking NHS aids has risen steadily since the service was commenced, and the median age is now over 70 years. The major type of hearing loss observed is sensorineural, but a substantial proportion of hearing aid candidates still have a significant conductive element in their hearing loss. This latter group of patients tend to be significantly younger than those without middle ear involvement. The hearing loss when applying for a hearing aid tends to be around 50 dB but is age dependent, younger subjects coming with smaller hearing losses. The most disturbing information obtained on questioning applicants was the long time lapse between recognition of hearing impairment and application for a hearing aid. This averaged almost ten years. The true delay is probably considerably greater than this and must have a substantial bearing on the outcome of hearing aid fitting. Public education and counselling of hearing aid users are suggested as vita needs."}
{"id": "PMID:486820", "title": "Continuing education and library services for physicians in office practice.", "content": "A program is described which incorporates library services into continuing medical education for physicians. The educational service is based on the actual needs of the physician rather than on his perceived needs. The needs assessment is accomplished by reviewing drug-prescribing habits. Current medical literature is then selected for the physician to coincide with his unique educational needs. The program is further designed to evaluate the change in the physician's drug-prescribing habits as a result of his study of the literature received.", "contents": "Continuing education and library services for physicians in office practice. A program is described which incorporates library services into continuing medical education for physicians. The educational service is based on the actual needs of the physician rather than on his perceived needs. The needs assessment is accomplished by reviewing drug-prescribing habits. Current medical literature is then selected for the physician to coincide with his unique educational needs. The program is further designed to evaluate the change in the physician's drug-prescribing habits as a result of his study of the literature received."}
{"id": "PMID:486821", "title": "Evaluation of manuscripts in health sciences librarianship.", "content": "Manuscripts submitted for review and full-length papers and brief communications published in the Bulletin of the Medical Library Association from 1976 through 1978 have been analyzed in order to identify some of the most common problems in the preparation of a paper. The findings point to a need for instructing librarians in (1) understanding the nature of \"explanation\" in a scientific or historical paper; (2) designing a research project and analyzing the data; (3) preparing data for display; and (4) more effective writing skills.", "contents": "Evaluation of manuscripts in health sciences librarianship. Manuscripts submitted for review and full-length papers and brief communications published in the Bulletin of the Medical Library Association from 1976 through 1978 have been analyzed in order to identify some of the most common problems in the preparation of a paper. The findings point to a need for instructing librarians in (1) understanding the nature of \"explanation\" in a scientific or historical paper; (2) designing a research project and analyzing the data; (3) preparing data for display; and (4) more effective writing skills."}
{"id": "PMID:486822", "title": "Profile, data, and management of two learning resource centers, 1970--1978.", "content": "Eight years' data have been collected in the operation of learning resource centers (LRCs) for medical and dental students at the Medical College of Virginia Campus, Virginia Commonwealth University. Data present evidence of high utilization of facilities and materials, including the successful use of small group cluster carrels. Management concepts and strategies to account for the data are identified and the LRC profile discussed for an understanding of how the strategies are implemented. These management concepts--responsiveness to students, interaction with faculty, and delivery of coordinated service--are transferable to other institutions and serve as guidlines for efficient management of LRC space, staff, equipment, and acquisition in support of medical and dental school curricula.", "contents": "Profile, data, and management of two learning resource centers, 1970--1978. Eight years' data have been collected in the operation of learning resource centers (LRCs) for medical and dental students at the Medical College of Virginia Campus, Virginia Commonwealth University. Data present evidence of high utilization of facilities and materials, including the successful use of small group cluster carrels. Management concepts and strategies to account for the data are identified and the LRC profile discussed for an understanding of how the strategies are implemented. These management concepts--responsiveness to students, interaction with faculty, and delivery of coordinated service--are transferable to other institutions and serve as guidlines for efficient management of LRC space, staff, equipment, and acquisition in support of medical and dental school curricula."}
{"id": "PMID:486823", "title": "New library buildings: Bracken Library, Queen's University, Kingston, Ontario, Canada.", "content": "A brief sketch is given of the past history and present functions of the Bracken Library. Architectural and decorative features of the new library are described.", "contents": "New library buildings: Bracken Library, Queen's University, Kingston, Ontario, Canada. A brief sketch is given of the past history and present functions of the Bracken Library. Architectural and decorative features of the new library are described."}
{"id": "PMID:486831", "title": "The jaundiced patient.", "content": "Jaundice is a rewarding condition to investigate because an explanation is usually found. A preliminary diagnosis based on the clinical features and liver function tests is made. Specialized investigations are then undertaken. In cholestasis or if the diagnosis is in doubt an obstructing lesion must be excluded. Ultrasonography should be performed first followed by PTC if the ducts appear dilated, ERCP may be the most useful examination in patients whose ducts are normal and in those who have had previous biliary surgery. If either PTC or ERCP fails to demonstrate the biliary system the other should be attempted. Liver biopsy should be performed before submitting an undiagnosed jaundiced patient to surgery.", "contents": "The jaundiced patient. Jaundice is a rewarding condition to investigate because an explanation is usually found. A preliminary diagnosis based on the clinical features and liver function tests is made. Specialized investigations are then undertaken. In cholestasis or if the diagnosis is in doubt an obstructing lesion must be excluded. Ultrasonography should be performed first followed by PTC if the ducts appear dilated, ERCP may be the most useful examination in patients whose ducts are normal and in those who have had previous biliary surgery. If either PTC or ERCP fails to demonstrate the biliary system the other should be attempted. Liver biopsy should be performed before submitting an undiagnosed jaundiced patient to surgery."}
{"id": "PMID:486832", "title": "Medical treatment of gallstones.", "content": "The last 10 years have witnessed an enormous amount of work on the medical dissolution of gallstones. Many compounds have been tested and one, chenodeoxycholic acid, is already available for clinical use in the UK. Others will certainly follow. Much remains to be learned of the mechanisms of action of these drugs and the safety of prolonged administration. Effective methods of preventing gallstone recurrence need to be devised. The practice of cholecystectomy is not yet threatened by medical dissolution of gallstones, but in selected patients medical treatment is now a viable alternative. A start has been made.", "contents": "Medical treatment of gallstones. The last 10 years have witnessed an enormous amount of work on the medical dissolution of gallstones. Many compounds have been tested and one, chenodeoxycholic acid, is already available for clinical use in the UK. Others will certainly follow. Much remains to be learned of the mechanisms of action of these drugs and the safety of prolonged administration. Effective methods of preventing gallstone recurrence need to be devised. The practice of cholecystectomy is not yet threatened by medical dissolution of gallstones, but in selected patients medical treatment is now a viable alternative. A start has been made."}
{"id": "PMID:486847", "title": "Changes in the brain catecholamines in patients with dementia of Alzheimer type.", "content": "Brain monoamine concentrations were determined post mortem in 19 patients with dementia of Alzheimer type. Samples were taken from 10 parts of the brain and compared with an age-matched control group. There were lower mean concentrations of dopamine in the demented group of patients in seven regions of the brain, and two of these were at a significant level. There were also significantly lower concentrations of homovanillic acid in the nucleus caudatus and in the putamen. The means of the concentrations of noradrenaline were also lower, and in the putamen and the cortex gyrus frontalis significant differnces were observed. The 5-hydroxytryptamine concentrations were slightly lower in the demented group but the differences did not reach significance. The degree of intellectual deterioration was negatively correlated with the noradrenaline concentrations in the hypothalamus and the cortex gyrus cinguli.", "contents": "Changes in the brain catecholamines in patients with dementia of Alzheimer type. Brain monoamine concentrations were determined post mortem in 19 patients with dementia of Alzheimer type. Samples were taken from 10 parts of the brain and compared with an age-matched control group. There were lower mean concentrations of dopamine in the demented group of patients in seven regions of the brain, and two of these were at a significant level. There were also significantly lower concentrations of homovanillic acid in the nucleus caudatus and in the putamen. The means of the concentrations of noradrenaline were also lower, and in the putamen and the cortex gyrus frontalis significant differnces were observed. The 5-hydroxytryptamine concentrations were slightly lower in the demented group but the differences did not reach significance. The degree of intellectual deterioration was negatively correlated with the noradrenaline concentrations in the hypothalamus and the cortex gyrus cinguli."}
{"id": "PMID:486848", "title": "Dichotic perception: evidence for right hemisphere dysfunction in affective psychosis.", "content": "The association between lateralized hemispheric dysfunction and psychiatric diagnosis was investigated with a measure of summation of dichotic clicks and with a dichotic competing message task. Ear asymmetries of affective psychotic patients were similar to those of right temporal lesioned controls and differed from those of schizophrenic patients and normal controls. In contrast, schizophrenic patients did not display abnormal auditory perceptual asymmetries.", "contents": "Dichotic perception: evidence for right hemisphere dysfunction in affective psychosis. The association between lateralized hemispheric dysfunction and psychiatric diagnosis was investigated with a measure of summation of dichotic clicks and with a dichotic competing message task. Ear asymmetries of affective psychotic patients were similar to those of right temporal lesioned controls and differed from those of schizophrenic patients and normal controls. In contrast, schizophrenic patients did not display abnormal auditory perceptual asymmetries."}
{"id": "PMID:486849", "title": "The use of Schneider's typology for the diagnosis of personality disorders--an examination of reliability.", "content": "Schneider's typology of the personality disorders is described briefly. Three psychiatrists made diagnoses on two sets of patients, both of which contained one example of each type. The diagnoses were made with the help of a glossary and based upon the examination of clinical summaries and audio-recordings. Values of two reliability coefficients showed considerable variation between types. High reliability was found for Schneider's asthenic, explosive, depressive and affectionless types. Low reliability was found for the fanatic, labile and hyperthymic types. The insecure and attention-seeking types were overused. Some possible causes of this variation are discussed.", "contents": "The use of Schneider's typology for the diagnosis of personality disorders--an examination of reliability. Schneider's typology of the personality disorders is described briefly. Three psychiatrists made diagnoses on two sets of patients, both of which contained one example of each type. The diagnoses were made with the help of a glossary and based upon the examination of clinical summaries and audio-recordings. Values of two reliability coefficients showed considerable variation between types. High reliability was found for Schneider's asthenic, explosive, depressive and affectionless types. Low reliability was found for the fanatic, labile and hyperthymic types. The insecure and attention-seeking types were overused. Some possible causes of this variation are discussed."}
{"id": "PMID:486850", "title": "The distinction between the affective psychoses and schizophrenia.", "content": "In an attempt to demonstrate a valid boundary between schizophrenia and the affective psychoses, discriminant function analyses have been carried out with history, mental state and follow-up data in two populations of patients. A bimodal distribution of discriminant scores was obtained in one of them (a general psychotic sample of 128 patients), using a discriminant function derived from the same sample; but when the function was applied to the second population (a schizoaffective sample of 106 patients) the distribution was ambiguous. Functions derived from the schizoaffective sample produced highly skewed distributions of discriminant scores in the general psychotic sample. Kraepelin's hypothesis that the functional psychoses consist of two distinct disease entities receives some support from our findings, but there is still no compelling evidence that the universe of psychotic patients falls naturally into these two groups.", "contents": "The distinction between the affective psychoses and schizophrenia. In an attempt to demonstrate a valid boundary between schizophrenia and the affective psychoses, discriminant function analyses have been carried out with history, mental state and follow-up data in two populations of patients. A bimodal distribution of discriminant scores was obtained in one of them (a general psychotic sample of 128 patients), using a discriminant function derived from the same sample; but when the function was applied to the second population (a schizoaffective sample of 106 patients) the distribution was ambiguous. Functions derived from the schizoaffective sample produced highly skewed distributions of discriminant scores in the general psychotic sample. Kraepelin's hypothesis that the functional psychoses consist of two distinct disease entities receives some support from our findings, but there is still no compelling evidence that the universe of psychotic patients falls naturally into these two groups."}
{"id": "PMID:486851", "title": "Thiamine and pyridoxine lack newly-admitted psychiatric patients.", "content": "One hundred and fifty four patients admitted to a general hospital psychiatric unit with a history of poor diet were examined. Serum pyruvate was estimated in all, red cell transketolase in 74 and red cell aspartate transaminase in 66. Significantly more of the 58 abnormally low thiamine patients than of the normal thiamine group showed clinical signs of malnutrition or were diagnosed as chronic alcoholics, drug addicts, schizophrenics or endogenous depressives. Significantly more endogenous depressives than other patients had a raised aspartate transaminase activity coefficient (pyridoxine lack.) While most low thiamine findings are probably manifestations of malnutrition pyridoxine lack may have some aetiological significance in endogenous depression.", "contents": "Thiamine and pyridoxine lack newly-admitted psychiatric patients. One hundred and fifty four patients admitted to a general hospital psychiatric unit with a history of poor diet were examined. Serum pyruvate was estimated in all, red cell transketolase in 74 and red cell aspartate transaminase in 66. Significantly more of the 58 abnormally low thiamine patients than of the normal thiamine group showed clinical signs of malnutrition or were diagnosed as chronic alcoholics, drug addicts, schizophrenics or endogenous depressives. Significantly more endogenous depressives than other patients had a raised aspartate transaminase activity coefficient (pyridoxine lack.) While most low thiamine findings are probably manifestations of malnutrition pyridoxine lack may have some aetiological significance in endogenous depression."}
{"id": "PMID:486852", "title": "Samaritan contact among 325 parasuicide patients.", "content": "With the aim of aiding primary prevention of parasuicide by Samaritans, a study of 325 parasuicide patients admitted to hospital was undertaken to discover the extent of knowledge about Samaritans, the proportion of patients who had contacted Samaritans and the reasons why the remainder had not done so. Ignorance about Samaritans was found in 28 per cent, such ignorance being significantly more common among teenagers than in other age groups. Only 1.4 per cent had sought help from Samaritans immediately before the present parasuicidal act, though a further 13 per cent had contacted Samaritans previously. Suggestions are made for future public education programmes about Samaritans.", "contents": "Samaritan contact among 325 parasuicide patients. With the aim of aiding primary prevention of parasuicide by Samaritans, a study of 325 parasuicide patients admitted to hospital was undertaken to discover the extent of knowledge about Samaritans, the proportion of patients who had contacted Samaritans and the reasons why the remainder had not done so. Ignorance about Samaritans was found in 28 per cent, such ignorance being significantly more common among teenagers than in other age groups. Only 1.4 per cent had sought help from Samaritans immediately before the present parasuicidal act, though a further 13 per cent had contacted Samaritans previously. Suggestions are made for future public education programmes about Samaritans."}
{"id": "PMID:486853", "title": "The prevalence and clinical presentation of psychiatric illness in a rural setting in Kenya.", "content": "One hundred and forty patients seen in a suburban walk-in walk-out clinic over a period of 30 days were studied. Twenty-eight (20 per cent) of them were found to be primarily psychiatrically disabled, 83 (59.3 per cent) primarily physically disabled and the diagnosis of the rest (20.7 per cent) was uncertain. The psychiatric patients were found to be suffering from anxiety and depressive states. Their sex, age, education, economic status, symptomatology and duration of the illness were analysed. The findings suggest among other things that the prevalence of psychiatric disorders in the rural setting in Kenya is high and therefore calls for a proportionate provision of mental health care facilities. They also suggest that anxiety and depression in African patients are masked by somatic symptoms, often leading to unnecessary delays in diagnosis and management.", "contents": "The prevalence and clinical presentation of psychiatric illness in a rural setting in Kenya. One hundred and forty patients seen in a suburban walk-in walk-out clinic over a period of 30 days were studied. Twenty-eight (20 per cent) of them were found to be primarily psychiatrically disabled, 83 (59.3 per cent) primarily physically disabled and the diagnosis of the rest (20.7 per cent) was uncertain. The psychiatric patients were found to be suffering from anxiety and depressive states. Their sex, age, education, economic status, symptomatology and duration of the illness were analysed. The findings suggest among other things that the prevalence of psychiatric disorders in the rural setting in Kenya is high and therefore calls for a proportionate provision of mental health care facilities. They also suggest that anxiety and depression in African patients are masked by somatic symptoms, often leading to unnecessary delays in diagnosis and management."}
{"id": "PMID:486854", "title": "A case of tranylcypromine ('Parnate') addiction.", "content": "A case of addiction to tranylcypromine is described where tolerance occurred and a severe withdrawal illness followed discontinuation of the drug. Previous reports in the literature of similar cases are reviewed and comparisons made, and the implications for management are discussed.", "contents": "A case of tranylcypromine ('Parnate') addiction. A case of addiction to tranylcypromine is described where tolerance occurred and a severe withdrawal illness followed discontinuation of the drug. Previous reports in the literature of similar cases are reviewed and comparisons made, and the implications for management are discussed."}
{"id": "PMID:486861", "title": "Meaning specificity in sentence processing.", "content": "Two experiments were conducted to test the principle of encoding specificity in sentence recognition when the target sentence was embedded in a meaningful discourse. The results indicate that change in meaning context resulted in significantly less recognition of the target sentences while change in sentence form and the target-test interval had no significant effects. The findings were discussed within the framework of a semantic interpretation of the encoding specificity principle.", "contents": "Meaning specificity in sentence processing. Two experiments were conducted to test the principle of encoding specificity in sentence recognition when the target sentence was embedded in a meaningful discourse. The results indicate that change in meaning context resulted in significantly less recognition of the target sentences while change in sentence form and the target-test interval had no significant effects. The findings were discussed within the framework of a semantic interpretation of the encoding specificity principle."}
{"id": "PMID:486862", "title": "Differential effects of noise and incentives on learning.", "content": "Three experiments are reported. In the first, monetary incentives improved the learning of nonsense words in response to colours only when the test order was the same as presentation order. In the second, incentives increased the recall of spatial location which served as an additional retrieval cue for nonsense words. In the third, noise was used to manipulate arousal. Noise during learning produced a significant decline in recall of locations for nonsense words. The results suggest that incentives increase attentional capacity, while noise does not. Previous results showing that noise increases the use of order cues are discussed and it is suggested that noise induces a type of learning which depends on order cues. Existing hypotheses about the nature of this process are noted but it is argued that further work is needed to select between them.", "contents": "Differential effects of noise and incentives on learning. Three experiments are reported. In the first, monetary incentives improved the learning of nonsense words in response to colours only when the test order was the same as presentation order. In the second, incentives increased the recall of spatial location which served as an additional retrieval cue for nonsense words. In the third, noise was used to manipulate arousal. Noise during learning produced a significant decline in recall of locations for nonsense words. The results suggest that incentives increase attentional capacity, while noise does not. Previous results showing that noise increases the use of order cues are discussed and it is suggested that noise induces a type of learning which depends on order cues. Existing hypotheses about the nature of this process are noted but it is argued that further work is needed to select between them."}
{"id": "PMID:486863", "title": "Self-rated imagery and encoding strategies in visual memory.", "content": "The value of self-rated vividness of imagery in predicting performance was investigated, taking into account the mnemonic strategies utilized among subjects performing a visual-memory task. Subjects classified as 'good' or 'poor' imagers, according to their scores in the Vividness of Visual Imagery Questionnaire (VVIQ; Marks, 1972), were to detect as rapidly as possible differences between pairs of similar pictures presented consecutively. No coding instructions were given and the mnemonic strategies used were analysed by studying subjective reports and objective performance measurements. The results indicated that the subjects utilized two main strategies--a detail or an image strategy. The detail strategy was the more efficient. In accordance with a previous study (Berger & Gaunitz, 1977), it was found that the VVIQ did not discriminate between performance by 'good' and 'poor' imagers. However, among subjects who used the image strategy, 'good' imagers performed more rapidly than 'poor' imagers. Self-rated imagery may then have some value in predicting performance among individuals shown to have utilized an image strategy.", "contents": "Self-rated imagery and encoding strategies in visual memory. The value of self-rated vividness of imagery in predicting performance was investigated, taking into account the mnemonic strategies utilized among subjects performing a visual-memory task. Subjects classified as 'good' or 'poor' imagers, according to their scores in the Vividness of Visual Imagery Questionnaire (VVIQ; Marks, 1972), were to detect as rapidly as possible differences between pairs of similar pictures presented consecutively. No coding instructions were given and the mnemonic strategies used were analysed by studying subjective reports and objective performance measurements. The results indicated that the subjects utilized two main strategies--a detail or an image strategy. The detail strategy was the more efficient. In accordance with a previous study (Berger & Gaunitz, 1977), it was found that the VVIQ did not discriminate between performance by 'good' and 'poor' imagers. However, among subjects who used the image strategy, 'good' imagers performed more rapidly than 'poor' imagers. Self-rated imagery may then have some value in predicting performance among individuals shown to have utilized an image strategy."}
{"id": "PMID:486864", "title": "Perception of simple figures: implicit-shape constancy and mental rotation.", "content": "Adults were shown simple figures either drawn on their own or on a face of a depicted cube. Under each of these conditions they were required to find identical figures in an array of stimuli of either the first or the second type. The response chosen determined the next stimulus used, the figures in both being identical. Four chains of 10 responses were thus obtained. Comparison of the choices reveals that the set consisting of 'plain' responses to 'cube' stimuli diverges significantly from the other three sets. This result is discussed in the context of other studies of implicit-shape constancy.", "contents": "Perception of simple figures: implicit-shape constancy and mental rotation. Adults were shown simple figures either drawn on their own or on a face of a depicted cube. Under each of these conditions they were required to find identical figures in an array of stimuli of either the first or the second type. The response chosen determined the next stimulus used, the figures in both being identical. Four chains of 10 responses were thus obtained. Comparison of the choices reveals that the set consisting of 'plain' responses to 'cube' stimuli diverges significantly from the other three sets. This result is discussed in the context of other studies of implicit-shape constancy."}
{"id": "PMID:486865", "title": "Individual differences in visual imaging and the voluntary control of heart rate.", "content": "The role of visual imagery in the bidirectional control of heart rate (HR) was explored in 24 subjects. While voluntary HR increases were reliably demonstrated with and without HR feedback, appropriate HR decreases were modest and inconsistent. Changes in respiration rate (RR) generally paralleled the alterations in HR. Eighteen of the subjects reported conventionally using visual imagery to effect HR changes. For these subjects, vividness of visual imagery scores and the extent of HR change showed indications of a direct relationship in the HR increase condition. However, in the HR decrease condition, it was concomitant RR changes which were associated with the vividness and the control of imagery production. The remaining six subjects, who reported not employing visual imagery, demonstrated superior HR acceleration to the imaging subjects. Further, while the imaging subjects did not benefit from the introduction of feedback, the six non-imaging subjects showed reliably greater HR acceleration as a result of feedback introduction.", "contents": "Individual differences in visual imaging and the voluntary control of heart rate. The role of visual imagery in the bidirectional control of heart rate (HR) was explored in 24 subjects. While voluntary HR increases were reliably demonstrated with and without HR feedback, appropriate HR decreases were modest and inconsistent. Changes in respiration rate (RR) generally paralleled the alterations in HR. Eighteen of the subjects reported conventionally using visual imagery to effect HR changes. For these subjects, vividness of visual imagery scores and the extent of HR change showed indications of a direct relationship in the HR increase condition. However, in the HR decrease condition, it was concomitant RR changes which were associated with the vividness and the control of imagery production. The remaining six subjects, who reported not employing visual imagery, demonstrated superior HR acceleration to the imaging subjects. Further, while the imaging subjects did not benefit from the introduction of feedback, the six non-imaging subjects showed reliably greater HR acceleration as a result of feedback introduction."}
{"id": "PMID:486866", "title": "Individual differences in reported visual imagery and memory performance.", "content": "Seventy high school students completed the Vividness of Visual Imagery Questionnaire (VVIQ). Two months later, the 16 highest scores (low visualizers) and the 16 lowest scores (high visualizers) took part in a memory experiment involving abstract words, concrete words and pictures. Analyses of variance showed that high visualizers were superior to low visualizers on all three kinds of item in short-term recall, whereas they were only superior on the concrete words and pictures in long-term recall. Finally, both groups performed equally well on a subsequent recognition test. The results were interpreted as providing support for the validity of the VVIQ.", "contents": "Individual differences in reported visual imagery and memory performance. Seventy high school students completed the Vividness of Visual Imagery Questionnaire (VVIQ). Two months later, the 16 highest scores (low visualizers) and the 16 lowest scores (high visualizers) took part in a memory experiment involving abstract words, concrete words and pictures. Analyses of variance showed that high visualizers were superior to low visualizers on all three kinds of item in short-term recall, whereas they were only superior on the concrete words and pictures in long-term recall. Finally, both groups performed equally well on a subsequent recognition test. The results were interpreted as providing support for the validity of the VVIQ."}
{"id": "PMID:486867", "title": "Variability in reading retardation.", "content": "It is argued that much of the confusion which has arisen as to the nature of dyslexia is because retarded readers have so frequently been studied as if they formed a qualitatively homogeneous group. It is suggested that in fact reading is not a unitary process, but that it involves the acquisition of a succession of skills; and that different retarded readers may break down at different stages in the acquisition of these. There may be types of retarded reader who are unable (1) to analyse complex visual shapes; or (2) to analyse whole word sounds into phonemes; or (3) to make simple regular grapheme-phoneme associations; or (4) to grasp irregularities in these; or (5) to group single words into phrases and sentences. Each of these disabilities may be associated with a particular type of deficiency in conceptual thinking.", "contents": "Variability in reading retardation. It is argued that much of the confusion which has arisen as to the nature of dyslexia is because retarded readers have so frequently been studied as if they formed a qualitatively homogeneous group. It is suggested that in fact reading is not a unitary process, but that it involves the acquisition of a succession of skills; and that different retarded readers may break down at different stages in the acquisition of these. There may be types of retarded reader who are unable (1) to analyse complex visual shapes; or (2) to analyse whole word sounds into phonemes; or (3) to make simple regular grapheme-phoneme associations; or (4) to grasp irregularities in these; or (5) to group single words into phrases and sentences. Each of these disabilities may be associated with a particular type of deficiency in conceptual thinking."}
{"id": "PMID:486868", "title": "Coordinating visual and kinaesthetic memory codes.", "content": "Four experiments examined the Connolly & Jones (1970) model which postulates that translation between modalities in the cross-modal paradigm occurs before storage in short-term memory. In general, the results provided no support for the translation notion. Delaying until the end of the retention interval knowledge of the reproduction mode failed to produce a matching performance decrement. Subjects were able to maintain the code of original presentation through the retention interval even when they did not expect reproduction to be in this mode. In addition, the asymmetry in the cross-modal matching of visual (V) and kinaesthetic (K) information, whereby K-V performance is more accurate than V-K performance, was found to occur only under certain visual display conditions. The implications of these findings for general models of cross-modal translation were discussed.", "contents": "Coordinating visual and kinaesthetic memory codes. Four experiments examined the Connolly & Jones (1970) model which postulates that translation between modalities in the cross-modal paradigm occurs before storage in short-term memory. In general, the results provided no support for the translation notion. Delaying until the end of the retention interval knowledge of the reproduction mode failed to produce a matching performance decrement. Subjects were able to maintain the code of original presentation through the retention interval even when they did not expect reproduction to be in this mode. In addition, the asymmetry in the cross-modal matching of visual (V) and kinaesthetic (K) information, whereby K-V performance is more accurate than V-K performance, was found to occur only under certain visual display conditions. The implications of these findings for general models of cross-modal translation were discussed."}
{"id": "PMID:486869", "title": "Short-term recall of nine-digit strings and the EEG.", "content": "In three exploratory studies, EEG changes were monitored while subjects performed a nine-digit recall task. Experiment 1 involved auditory presentation without practice or prior instruction. EEG characteristics measured before the task related to subsequent recall, EEG activation increased progressively during digit presentation and rehearsal, and several between- and within-subject analyses showed increased activation to be associated with poor recall performance. In Expt. 2 the digits were presented visually and subjects were given instruction and practice in an efficient rehearsal strategy. Recall performance improved but its relationship with the pre-task EEG disappeared. Activation increased with digit presentation but the relationship between the EEG and performance was complex since a within-subject analysis associated decreased activation with better performance (as in Expt. 1) while this relationship was reversed in a between-subject analysis. The results are explained both in terms of traditional findings relating activation to recall, and drive theory accounts of learning and performance. Finally, Expt. 3 demonstrated that performance was better, both in the morning (compared with the evening) and on a second testing session. There were complex time of day effects for the EEG, with lower and higher measured frequencies yielding higher voltage output in the evening and intermediate frequencies showing a reverse effect. In summarizing the data from the three experiments it is suggested that different EEG frequencies are differentially sensitive to different conditions (task specific factors, stimulus input characteristics, knowledge of success and failure, degree of task mastery and circadian variation).", "contents": "Short-term recall of nine-digit strings and the EEG. In three exploratory studies, EEG changes were monitored while subjects performed a nine-digit recall task. Experiment 1 involved auditory presentation without practice or prior instruction. EEG characteristics measured before the task related to subsequent recall, EEG activation increased progressively during digit presentation and rehearsal, and several between- and within-subject analyses showed increased activation to be associated with poor recall performance. In Expt. 2 the digits were presented visually and subjects were given instruction and practice in an efficient rehearsal strategy. Recall performance improved but its relationship with the pre-task EEG disappeared. Activation increased with digit presentation but the relationship between the EEG and performance was complex since a within-subject analysis associated decreased activation with better performance (as in Expt. 1) while this relationship was reversed in a between-subject analysis. The results are explained both in terms of traditional findings relating activation to recall, and drive theory accounts of learning and performance. Finally, Expt. 3 demonstrated that performance was better, both in the morning (compared with the evening) and on a second testing session. There were complex time of day effects for the EEG, with lower and higher measured frequencies yielding higher voltage output in the evening and intermediate frequencies showing a reverse effect. In summarizing the data from the three experiments it is suggested that different EEG frequencies are differentially sensitive to different conditions (task specific factors, stimulus input characteristics, knowledge of success and failure, degree of task mastery and circadian variation)."}
{"id": "PMID:486870", "title": "The influence of lateral organization on the evaluation of paintings.", "content": "This study examines the influence of directional properties of lateral organization on evaluation of paintings. Lateral organization, as defined here, has two dimensions: (1) left or right location of the principal figure; and (2) left-to-right or right-to-left sequence of figures represented by the direction of suggested movement, action, or lines. That previous studies have failed to demonstrate consistently a relationship between evaluations and directional properties is attributed to methodological shortcomings involving lack of control of stimulus properties and exposure time. Here, on the basis of a well-defined judging procedure, paintings were distinguished according to whether each dimension of lateral organization depicted one or more than one direction. Under two exposure conditions, 5 and 20 s, three evaluations (general preference, balance, and dynamics) were made for paintings which were presented simultaneously in the artist-created, original view, and the mirror-image, reversed view. It was found that evaluations were influenced by directional properties (sequence of figures) with a 5 s exposure and by more inclusive formal features with a 20 s exposure. Implications of the findings for perception and aesthetics are considered.", "contents": "The influence of lateral organization on the evaluation of paintings. This study examines the influence of directional properties of lateral organization on evaluation of paintings. Lateral organization, as defined here, has two dimensions: (1) left or right location of the principal figure; and (2) left-to-right or right-to-left sequence of figures represented by the direction of suggested movement, action, or lines. That previous studies have failed to demonstrate consistently a relationship between evaluations and directional properties is attributed to methodological shortcomings involving lack of control of stimulus properties and exposure time. Here, on the basis of a well-defined judging procedure, paintings were distinguished according to whether each dimension of lateral organization depicted one or more than one direction. Under two exposure conditions, 5 and 20 s, three evaluations (general preference, balance, and dynamics) were made for paintings which were presented simultaneously in the artist-created, original view, and the mirror-image, reversed view. It was found that evaluations were influenced by directional properties (sequence of figures) with a 5 s exposure and by more inclusive formal features with a 20 s exposure. Implications of the findings for perception and aesthetics are considered."}
{"id": "PMID:486871", "title": "Dimensions and determinants of judgements of colour samples and a simulated interior space by architects and non-architects.", "content": "Architects and non-architects made Semantic Differential ratings of colour samples (chips) and a simulated interior space (a model). In analyses of the total samples' ratings (architects and non-architects) of (a) colour chips and (b) models, and individual sample analyses, (c) architects' chip judgements, (d) architects' model judgements, (e) non-architects' chip judgements, and (f) non-architects' model judgements, five factors occurred, though not necessarily all in any one analysis. These were: (i) dynamism; (ii) spatial quality; (iii) emotional tone; (iv) evaluation; (v) complexity. Linear correlations between parameters of the Munsell Color System and the above factors in the various analyses were calculated, while parallel analyses were carried out employing a graphical technique described by Sivik (1974a) involving isosemantic maps. In all analyses, linear correlations between the colour parameters and judgements were found for the dynamism factor, spatial quality factor, and emotional tone factor. They were associated respectively with chroma, value, and hue. Inspection of the isosemantic maps indicated subsidiary effects of the non-dominant dimensions of a non-linear sort, though the maps also exhibited the linear relations. Linear correlations were low or non-existent for the evaluation and complexity factors, and the complex nature of their determinants was clear from the isosemantic maps. The dterminants of judgements were similar for architects and non-architects, with the exception of evaluative judgements for the models in which markedly different determinants were noted. Comparability of the present findings with other studies carried out in a variety of countries over a 20 year period was high for dynamism, spatial quality, and emotional tone, and it is suggested that there may be something inherent in the response to colour in relation to such judgements. Recent physiological work is discussed, and its limitations in terms of colours sampled and an overconcentration on the hue dimension noted. In contrast, it is suggested that dimensions of judgement, such as evaluation or complexity, reflect to a greater extent culture or training, and are hence independent of the basic colour attributes.", "contents": "Dimensions and determinants of judgements of colour samples and a simulated interior space by architects and non-architects. Architects and non-architects made Semantic Differential ratings of colour samples (chips) and a simulated interior space (a model). In analyses of the total samples' ratings (architects and non-architects) of (a) colour chips and (b) models, and individual sample analyses, (c) architects' chip judgements, (d) architects' model judgements, (e) non-architects' chip judgements, and (f) non-architects' model judgements, five factors occurred, though not necessarily all in any one analysis. These were: (i) dynamism; (ii) spatial quality; (iii) emotional tone; (iv) evaluation; (v) complexity. Linear correlations between parameters of the Munsell Color System and the above factors in the various analyses were calculated, while parallel analyses were carried out employing a graphical technique described by Sivik (1974a) involving isosemantic maps. In all analyses, linear correlations between the colour parameters and judgements were found for the dynamism factor, spatial quality factor, and emotional tone factor. They were associated respectively with chroma, value, and hue. Inspection of the isosemantic maps indicated subsidiary effects of the non-dominant dimensions of a non-linear sort, though the maps also exhibited the linear relations. Linear correlations were low or non-existent for the evaluation and complexity factors, and the complex nature of their determinants was clear from the isosemantic maps. The dterminants of judgements were similar for architects and non-architects, with the exception of evaluative judgements for the models in which markedly different determinants were noted. Comparability of the present findings with other studies carried out in a variety of countries over a 20 year period was high for dynamism, spatial quality, and emotional tone, and it is suggested that there may be something inherent in the response to colour in relation to such judgements. Recent physiological work is discussed, and its limitations in terms of colours sampled and an overconcentration on the hue dimension noted. In contrast, it is suggested that dimensions of judgement, such as evaluation or complexity, reflect to a greater extent culture or training, and are hence independent of the basic colour attributes."}
{"id": "PMID:486872", "title": "Emergence of unreported stimuli into imagery as a function of laterality of presentation: a replication and extension of research by Henley & Dixon (1974).", "content": "In Expt. 1, the research by Henley & Dixon (1974) was replicated. Two experimental groups (n=8 each) received subliminal words to the right ear and 8 min of music to the left ear or vice versa. Each group had its own control (n=8 each) with only music to one ear. Categorization and ranking of imagery reported, as well as performance on a checklist containing critical, associated, and non-related words, showed that emergence was greater with words to the right ear than with no words. There was no difference between experimental and control groups with words to the left ear. In Expt. 2, the music was eliminated, and subjects received subliminal words to the right ear (n=8) or no words. A significant difference between groups was found when the reported imagery was ranked as to emergence, and on the checklist, but not when the reports were categorized by judges.", "contents": "Emergence of unreported stimuli into imagery as a function of laterality of presentation: a replication and extension of research by Henley & Dixon (1974). In Expt. 1, the research by Henley & Dixon (1974) was replicated. Two experimental groups (n=8 each) received subliminal words to the right ear and 8 min of music to the left ear or vice versa. Each group had its own control (n=8 each) with only music to one ear. Categorization and ranking of imagery reported, as well as performance on a checklist containing critical, associated, and non-related words, showed that emergence was greater with words to the right ear than with no words. There was no difference between experimental and control groups with words to the left ear. In Expt. 2, the music was eliminated, and subjects received subliminal words to the right ear (n=8) or no words. A significant difference between groups was found when the reported imagery was ranked as to emergence, and on the checklist, but not when the reports were categorized by judges."}
{"id": "PMID:486873", "title": "Non-verbal and verbal judgements of length invariance by young children.", "content": "One hundred and fifty non-conservers in length were assessed for their ability to make a non-verbal judgement of length invariance on a task employing a train-transfer design in which the stimuli were pairs of pencils. Seventy per cent of the children from the group who had to respond to length equality as opposed to inequality made the correct invariance judgement and could, moreover, characterize their choices in invariance language (e.g. 'same size') whilst still failing the standard verbal task in which such phrases were used by the experimenter. This is regarded as supporting the hypothesis that the non-conserver's linguistic difficulty is not with framing the verbal judgement but in interpreting a question which he believes has to be interpreted unidimensionally in such a context of perceptual change.", "contents": "Non-verbal and verbal judgements of length invariance by young children. One hundred and fifty non-conservers in length were assessed for their ability to make a non-verbal judgement of length invariance on a task employing a train-transfer design in which the stimuli were pairs of pencils. Seventy per cent of the children from the group who had to respond to length equality as opposed to inequality made the correct invariance judgement and could, moreover, characterize their choices in invariance language (e.g. 'same size') whilst still failing the standard verbal task in which such phrases were used by the experimenter. This is regarded as supporting the hypothesis that the non-conserver's linguistic difficulty is not with framing the verbal judgement but in interpreting a question which he believes has to be interpreted unidimensionally in such a context of perceptual change."}
{"id": "PMID:486874", "title": "On the nature of difficulties in spatial-perceptual tasks: ethnic and sex differences.", "content": "The common claim that Africans exhibit a general weakness in handling spatial relationships is questioned, as is that of a general male superiority. The aim of the study was to identify some of the specific areas in which ethnic and sex differences may be located. A sample of 72 Ghanaian children evenly divided according to sex and early, middle and later stages of primary schooling were administered a series of tasks adapted from Piaget & Inhelder (1971); the same tasks were given to an equivalent group of Scottish children. On a block construction task involving memory the Ghanaians performed at the same level as the Scots when working from models, but not when the presentation consisted of photographs or line drawings (P less than 0.001). On two tasks requiring mental rotation of shapes and, subsequently, the actual assembly of shapes into regular figures, the Ghanaians again experienced more difficulty (P less than 0.001). Intercorrelation of scores on the various tasks suggested that the underlying structures of abilities may differ in Ghana and Scotland. The pattern of sex differences was exactly the same in both cultures. Girls did less well with the block construction (P less than 0.001), but there was no significant sex difference on mental rotation or shape assembly.", "contents": "On the nature of difficulties in spatial-perceptual tasks: ethnic and sex differences. The common claim that Africans exhibit a general weakness in handling spatial relationships is questioned, as is that of a general male superiority. The aim of the study was to identify some of the specific areas in which ethnic and sex differences may be located. A sample of 72 Ghanaian children evenly divided according to sex and early, middle and later stages of primary schooling were administered a series of tasks adapted from Piaget & Inhelder (1971); the same tasks were given to an equivalent group of Scottish children. On a block construction task involving memory the Ghanaians performed at the same level as the Scots when working from models, but not when the presentation consisted of photographs or line drawings (P less than 0.001). On two tasks requiring mental rotation of shapes and, subsequently, the actual assembly of shapes into regular figures, the Ghanaians again experienced more difficulty (P less than 0.001). Intercorrelation of scores on the various tasks suggested that the underlying structures of abilities may differ in Ghana and Scotland. The pattern of sex differences was exactly the same in both cultures. Girls did less well with the block construction (P less than 0.001), but there was no significant sex difference on mental rotation or shape assembly."}
{"id": "PMID:486875", "title": "How specific are perceptual skills? A cross-cultural study of pattern reproduction.", "content": "Pattern reproduction tasks were presented in four different media to samples of urban Zambian and urban British schoolchildren. When the patterns were reproduced as wire models, the Zambian children excelled the British. When the patterns were reproduced by drawing, the British children excelled the Zambian. No reliable cross-cultural differences were found when the patterns were reproduced as plasticine models or as configurations of hand positions. Both cultural groups were equally adversely affected when required to perform the modelling tasks or the hand positions task blind-folded. The results are interpreted as suggesting that cross-cultural differences in performance of pattern reproduction tasks reflect different sets of highly specific perceptual skills rather than differences on broader cognitive variables such as practical intelligence, field-dependency or sensotypes.", "contents": "How specific are perceptual skills? A cross-cultural study of pattern reproduction. Pattern reproduction tasks were presented in four different media to samples of urban Zambian and urban British schoolchildren. When the patterns were reproduced as wire models, the Zambian children excelled the British. When the patterns were reproduced by drawing, the British children excelled the Zambian. No reliable cross-cultural differences were found when the patterns were reproduced as plasticine models or as configurations of hand positions. Both cultural groups were equally adversely affected when required to perform the modelling tasks or the hand positions task blind-folded. The results are interpreted as suggesting that cross-cultural differences in performance of pattern reproduction tasks reflect different sets of highly specific perceptual skills rather than differences on broader cognitive variables such as practical intelligence, field-dependency or sensotypes."}
{"id": "PMID:486876", "title": "Interocular transfer of movement after-effects in schizophrenia.", "content": "A group of 16 chronic schizophrenic subjects were compared with 15 age-matched control subjects for interocular transfer of movement after-effects. Contrary to the hypothesis that schizophrenic subjects would show a deficit on this measure schizophrenics showed increased transfer compared to the controls. This effect is not due to response perseveration and is not correlated with length of hospitalization, age or dose of antipsychotic drugs. It is suggested that the effect reflects a deficit in 'inhibitory processes' in schizophrenia.", "contents": "Interocular transfer of movement after-effects in schizophrenia. A group of 16 chronic schizophrenic subjects were compared with 15 age-matched control subjects for interocular transfer of movement after-effects. Contrary to the hypothesis that schizophrenic subjects would show a deficit on this measure schizophrenics showed increased transfer compared to the controls. This effect is not due to response perseveration and is not correlated with length of hospitalization, age or dose of antipsychotic drugs. It is suggested that the effect reflects a deficit in 'inhibitory processes' in schizophrenia."}
{"id": "PMID:486877", "title": "Individual differences in right and left reaction time.", "content": "Experiments designed to check the absence of effects for hands and handedness in simple and two-choice reaction time found unexpected individual differences related to stimulus laterality. The majority of subjects responded faster to the stimulus on the left and a substantial minority responded faster to the stimulus on the right in any choice pair. The right index finger was slower than the left index or the middle fingers. Choices tended to be faster between fingers on different hands than on the same hand and same-hand choices were faster with the left hand than the right hand. There were no effects attributable to hand preference or sex.", "contents": "Individual differences in right and left reaction time. Experiments designed to check the absence of effects for hands and handedness in simple and two-choice reaction time found unexpected individual differences related to stimulus laterality. The majority of subjects responded faster to the stimulus on the left and a substantial minority responded faster to the stimulus on the right in any choice pair. The right index finger was slower than the left index or the middle fingers. Choices tended to be faster between fingers on different hands than on the same hand and same-hand choices were faster with the left hand than the right hand. There were no effects attributable to hand preference or sex."}
{"id": "PMID:486879", "title": "Emotionality in male and female rodents: a reply to Archer.", "content": "Archer (1979) has complained that his criticisms (Archer, 1971, 1973, 1975) of Gray's (1971 a, b) views on emotionality and on sex differences in fearfulness in rodents have not been answered. These criticisms are shown to lack substance and to depend on confused arguments and misleading reviews of the literature.", "contents": "Emotionality in male and female rodents: a reply to Archer. Archer (1979) has complained that his criticisms (Archer, 1971, 1973, 1975) of Gray's (1971 a, b) views on emotionality and on sex differences in fearfulness in rodents have not been answered. These criticisms are shown to lack substance and to depend on confused arguments and misleading reviews of the literature."}
{"id": "PMID:486880", "title": "Negative recency and measurement of primary memory.", "content": "The technique of Waugh & Norman (1965) is widely used in measuring the capacity of primary memory. Watkins (1974) has proposed a modification of the technique which takes into account registration failure. A further modification is proposed which takes into account findings of negative recency in delayed recall.", "contents": "Negative recency and measurement of primary memory. The technique of Waugh & Norman (1965) is widely used in measuring the capacity of primary memory. Watkins (1974) has proposed a modification of the technique which takes into account registration failure. A further modification is proposed which takes into account findings of negative recency in delayed recall."}
{"id": "PMID:486881", "title": "Fitness assessment of English league soccer players through the competitive season.", "content": "A battery of 26 tests was administered to professional soccer players (n = 31) at 3 points during the competitive season. Significant changes from start to mid-season were limited to improved muscular power, increased resting heart rate (fH), decreased force expiratory flow (FEF) and ankle mobility. No further changes with the duration of the competitive season were observed. Differences between squads were predominantly cardiac function measures at mid-season, and strength, muscular power and limb girths at the end of the season. The First Team squad had consistently less fat and poorer ankle mobility. It seems that mean fitness levels remain relatively stable during the playing season, though the measures significantly discriminating between the top and reserve squads fluctuate.", "contents": "Fitness assessment of English league soccer players through the competitive season. A battery of 26 tests was administered to professional soccer players (n = 31) at 3 points during the competitive season. Significant changes from start to mid-season were limited to improved muscular power, increased resting heart rate (fH), decreased force expiratory flow (FEF) and ankle mobility. No further changes with the duration of the competitive season were observed. Differences between squads were predominantly cardiac function measures at mid-season, and strength, muscular power and limb girths at the end of the season. The First Team squad had consistently less fat and poorer ankle mobility. It seems that mean fitness levels remain relatively stable during the playing season, though the measures significantly discriminating between the top and reserve squads fluctuate."}
{"id": "PMID:486882", "title": "The effect of regular exercise on cognitive functioning and personality.", "content": "The effect of regular exercise on cognitive functioning and personality was investigated in 32 subjects representing 4 discrete groups based on sex and age. Before and after a 10 week exercise programme of jogging, calisthenics, and recreational activities, a test battery was administered to assess functioning in a number of domains: intelligence (WAIS Digit Symbol and Block Design); brain function (Trail-Making); speed of performance (Crossing-Off); memory and learning (WMS Visual Reproduction and Associate Learning); morale and life satisfaction (Life Satisfaction and Control Ratings); anxiety (MAACL); and depression (MAACL). Improvement was observed on several physiological parameters. ANOVA revealed significant sex and age differences on Digit Symbol and Block Design and age differences on Trail-Making, Crossing-Off, Associate Learning, and anxiety. Regardless of sex and age, significant improvement in performance was observed from pre to post-test on Digit Symbol, Block Design, Trail-Making, Crossing-Off, and on Associate Learning. In addition, an increase on health status rating (p less than .01) and decrease in anxiety were observed from pre to post-test. These data illustrate beneficial effects of exercise on certain measures of cognitive functioning and personality.", "contents": "The effect of regular exercise on cognitive functioning and personality. The effect of regular exercise on cognitive functioning and personality was investigated in 32 subjects representing 4 discrete groups based on sex and age. Before and after a 10 week exercise programme of jogging, calisthenics, and recreational activities, a test battery was administered to assess functioning in a number of domains: intelligence (WAIS Digit Symbol and Block Design); brain function (Trail-Making); speed of performance (Crossing-Off); memory and learning (WMS Visual Reproduction and Associate Learning); morale and life satisfaction (Life Satisfaction and Control Ratings); anxiety (MAACL); and depression (MAACL). Improvement was observed on several physiological parameters. ANOVA revealed significant sex and age differences on Digit Symbol and Block Design and age differences on Trail-Making, Crossing-Off, Associate Learning, and anxiety. Regardless of sex and age, significant improvement in performance was observed from pre to post-test on Digit Symbol, Block Design, Trail-Making, Crossing-Off, and on Associate Learning. In addition, an increase on health status rating (p less than .01) and decrease in anxiety were observed from pre to post-test. These data illustrate beneficial effects of exercise on certain measures of cognitive functioning and personality."}
{"id": "PMID:486884", "title": "A pilot study on left ventricular dimensions and wall stress before and after submaximal exercise.", "content": "Left ventricle dimensions and wall stress were measured echocardiographically before and immediately after exercise in 14 athletes and 7 control subjects. Our findings suggest that afterload is an important determinant of cardiac performance and wall hypertrophy in athletes. In spite of major changes in heart rate and blood pressure, left ventricular wall stress remains unchanged following submaximal exercise, in trained and untrained hearts. It would appear that the changes in heart size during exercise are to a large extent limited in untrained ventricles, as smaller left ventricular dimensions are required, to \"normalise\" wall stress. This results in a lower stroke volume for a given stroke dimensional change. Consequently cardiac output is a function of heart rate rather than stroke volume in untrained subjects. The effect of increased muscle mass in athletes, is to permit larger left ventricular dimensions for a given afterload, thus stroke volume can be augmented. The increase h/R ratio suggests that afterload is more important than preload in the development of left ventricular hypertrophy in rowers and swimmers.", "contents": "A pilot study on left ventricular dimensions and wall stress before and after submaximal exercise. Left ventricle dimensions and wall stress were measured echocardiographically before and immediately after exercise in 14 athletes and 7 control subjects. Our findings suggest that afterload is an important determinant of cardiac performance and wall hypertrophy in athletes. In spite of major changes in heart rate and blood pressure, left ventricular wall stress remains unchanged following submaximal exercise, in trained and untrained hearts. It would appear that the changes in heart size during exercise are to a large extent limited in untrained ventricles, as smaller left ventricular dimensions are required, to \"normalise\" wall stress. This results in a lower stroke volume for a given stroke dimensional change. Consequently cardiac output is a function of heart rate rather than stroke volume in untrained subjects. The effect of increased muscle mass in athletes, is to permit larger left ventricular dimensions for a given afterload, thus stroke volume can be augmented. The increase h/R ratio suggests that afterload is more important than preload in the development of left ventricular hypertrophy in rowers and swimmers."}
{"id": "PMID:486888", "title": "Athletes' leg pains.", "content": "The frequency and nature of exertion pains of the leg in athletes were studied in 2,750 cases of overuse injuries treated at the Sports Clinic of the Deaconess Institute of Oulu, Finland, during the years 1972-1977. 465 cases of exertion pain (18%) were located in the shin. The medial tibial syndrome was the most common overuse injury among these athletes, comprising 9.5% of all exertion injuries and 60% of the leg exertion pains. Together with stress fracture of the tibia, the second most common exertion pain of the leg, it accounted for 75% of the total leg pains. There are certain difficulties in differentiating between the medial tibial syndrome and stress fracture of the tibia. They both occur at the same site with similar symptoms. Radiological examination and isotope scanning are needed. The medial tibial syndrome is an overuse injury at the medial tibial border caused by running exercises. The pain is elicited by exertional ischaemia. The pathogenesis is explained by increased pressure in the fascial compartment of the deep flexor muscles due to prolonged exercise. Similar chronic ischaemic pains from exercise are also found in other fascial compartments of the leg, especially in the anterior compartment. The only treatment needed for stress fractures is rest from training. Fascial compartment pains also usually subside. If chronic fascial syndromes prevent training, fasciotomy is recommended as a reliable method to restore the athlete to normal training without pains.", "contents": "Athletes' leg pains. The frequency and nature of exertion pains of the leg in athletes were studied in 2,750 cases of overuse injuries treated at the Sports Clinic of the Deaconess Institute of Oulu, Finland, during the years 1972-1977. 465 cases of exertion pain (18%) were located in the shin. The medial tibial syndrome was the most common overuse injury among these athletes, comprising 9.5% of all exertion injuries and 60% of the leg exertion pains. Together with stress fracture of the tibia, the second most common exertion pain of the leg, it accounted for 75% of the total leg pains. There are certain difficulties in differentiating between the medial tibial syndrome and stress fracture of the tibia. They both occur at the same site with similar symptoms. Radiological examination and isotope scanning are needed. The medial tibial syndrome is an overuse injury at the medial tibial border caused by running exercises. The pain is elicited by exertional ischaemia. The pathogenesis is explained by increased pressure in the fascial compartment of the deep flexor muscles due to prolonged exercise. Similar chronic ischaemic pains from exercise are also found in other fascial compartments of the leg, especially in the anterior compartment. The only treatment needed for stress fractures is rest from training. Fascial compartment pains also usually subside. If chronic fascial syndromes prevent training, fasciotomy is recommended as a reliable method to restore the athlete to normal training without pains."}
{"id": "PMID:486890", "title": "High definition imaging of carotid arteries using a standard commercial ultrasound \"B\" scanner. A preliminary report.", "content": "Sixteen carotid arteries in nine patients with transient ischaemic attacks and carotid bruits were examined by direct scanning grey scale ultrasound using a 10 HMz probe attached to a standard commercial apparatus. Findings using ultrasound were compared with a contrast arteriogram performed on the following day and, in two cases, with the surgical appearances during endarterectomy. Atheromatous plaques were demonstrated in five common carotid arteries by ultrasound. Arteriography failed to show proximal plaques in two common carotid arteries and extension of bifurcation disease into the adjacent common carotid in a third patient. Ultrasound detected four stenotic and one occluded internal carotid artery. One internal carotid artery with a 25% stenosis was shown by arteriography but not by ultrasound. Of the five external carotid stenoses, one with a minimal stenosis of less than 10% was not demonstrated. In all cases, stenoses in excess of 30% were shown by ultrasound. Surgical findings correlated more closely with details of the plaques as shown by ultrasound than by arteriography. It is suggested that ultrasound might be used to pre-select reliably those patients who would benefit from a contrast arteriogram and, thus, to protect those with normal carotid arteries from a potentially hazardous invasive investigation.", "contents": "High definition imaging of carotid arteries using a standard commercial ultrasound \"B\" scanner. A preliminary report. Sixteen carotid arteries in nine patients with transient ischaemic attacks and carotid bruits were examined by direct scanning grey scale ultrasound using a 10 HMz probe attached to a standard commercial apparatus. Findings using ultrasound were compared with a contrast arteriogram performed on the following day and, in two cases, with the surgical appearances during endarterectomy. Atheromatous plaques were demonstrated in five common carotid arteries by ultrasound. Arteriography failed to show proximal plaques in two common carotid arteries and extension of bifurcation disease into the adjacent common carotid in a third patient. Ultrasound detected four stenotic and one occluded internal carotid artery. One internal carotid artery with a 25% stenosis was shown by arteriography but not by ultrasound. Of the five external carotid stenoses, one with a minimal stenosis of less than 10% was not demonstrated. In all cases, stenoses in excess of 30% were shown by ultrasound. Surgical findings correlated more closely with details of the plaques as shown by ultrasound than by arteriography. It is suggested that ultrasound might be used to pre-select reliably those patients who would benefit from a contrast arteriogram and, thus, to protect those with normal carotid arteries from a potentially hazardous invasive investigation."}
{"id": "PMID:486891", "title": "Contrast dose for enhancement of computed tomograms of the brain.", "content": "This study shows that contrast enhancement of cerebral lesions can be adequately achieved with a bolus injection of 60 ml of sodium iothalamate 70%. While the infusion of a larger dose of contrast agent increases quantitatively the level of enhancement of a variety of lesions, this method of examination does not appear to provide any more useful diagnostic information, and it introduces added and unnecessary inconvenience, cost and potential hazard.", "contents": "Contrast dose for enhancement of computed tomograms of the brain. This study shows that contrast enhancement of cerebral lesions can be adequately achieved with a bolus injection of 60 ml of sodium iothalamate 70%. While the infusion of a larger dose of contrast agent increases quantitatively the level of enhancement of a variety of lesions, this method of examination does not appear to provide any more useful diagnostic information, and it introduces added and unnecessary inconvenience, cost and potential hazard."}
{"id": "PMID:486892", "title": "A quantitative evaluation of film and film/screen combinations for mammographic examination.", "content": "A test phantom containing simulated micro-calcifications was constructed. This was imaged by seven systems suitable for use in mammography, firstly in scatter-free conditions and secondly with the addition of tissue-equivalent scattering material. The ability of the systems to resolve micro-particles of known size was assessed using the techniques of signal detection theory. The resultant receiver operating characteristic curves separated the systems into three major groups. Firstly, high resolution systems requiring high radiation dose techniques; secondly at the other extreme, a mediocre imaging system producing its results at low radiation levels; and thirdly a larger middle group producing intermediate resolution of fine particles at similar, acceptable dose levels. The ability to image small particles does not increase linearly with the radiation dose. Scatter did not affect all of the systems to the same extent.", "contents": "A quantitative evaluation of film and film/screen combinations for mammographic examination. A test phantom containing simulated micro-calcifications was constructed. This was imaged by seven systems suitable for use in mammography, firstly in scatter-free conditions and secondly with the addition of tissue-equivalent scattering material. The ability of the systems to resolve micro-particles of known size was assessed using the techniques of signal detection theory. The resultant receiver operating characteristic curves separated the systems into three major groups. Firstly, high resolution systems requiring high radiation dose techniques; secondly at the other extreme, a mediocre imaging system producing its results at low radiation levels; and thirdly a larger middle group producing intermediate resolution of fine particles at similar, acceptable dose levels. The ability to image small particles does not increase linearly with the radiation dose. Scatter did not affect all of the systems to the same extent."}
{"id": "PMID:486893", "title": "The effect of recovery from potentially lethal damage on the determination of reoxygenation in a murine tumour.", "content": "The pattern of reoxygenation in the murine anaplastic MT tumour was investigated using the established method of determining the hypoxic fraction, at intervals after a priming X-ray dose, from test doses given either to unclamped or clamped-off tumours. Little reoxygenation was apparent whilst the tumour was increasing in size for 12--72 hours after a single dose of 20.3 Gy, but extensive reoxygenation was evident whilst the tumour was shrinking at nine days after a dose of 50 Gy. However, the degree of reoxygenation may have been underestimated, especially after the smaller priming dose. This is because only the chronically hypoxic cells in this tumour have the ability to recover from potentially lethal damage (PLD) and so are more radioresistant than cells rendered acutely hypoxic by clamping. Because of this, even when tumours are clamped off during irradiation, the resulting survival curve is biphasic and the apparent effect of the clamp becomes a function of the X-ray dose used. The larger the dose, the smaller the observed effect of the clamp, so the greater the apparent hypoxic fraction and hence the smaller the apparent degree of reoxygenation.", "contents": "The effect of recovery from potentially lethal damage on the determination of reoxygenation in a murine tumour. The pattern of reoxygenation in the murine anaplastic MT tumour was investigated using the established method of determining the hypoxic fraction, at intervals after a priming X-ray dose, from test doses given either to unclamped or clamped-off tumours. Little reoxygenation was apparent whilst the tumour was increasing in size for 12--72 hours after a single dose of 20.3 Gy, but extensive reoxygenation was evident whilst the tumour was shrinking at nine days after a dose of 50 Gy. However, the degree of reoxygenation may have been underestimated, especially after the smaller priming dose. This is because only the chronically hypoxic cells in this tumour have the ability to recover from potentially lethal damage (PLD) and so are more radioresistant than cells rendered acutely hypoxic by clamping. Because of this, even when tumours are clamped off during irradiation, the resulting survival curve is biphasic and the apparent effect of the clamp becomes a function of the X-ray dose used. The larger the dose, the smaller the observed effect of the clamp, so the greater the apparent hypoxic fraction and hence the smaller the apparent degree of reoxygenation."}
{"id": "PMID:486896", "title": "The effect of local hyperthermia on the small intestine of the mouse.", "content": "Small loops of mouse jejunum were exteriorized and heated by immersion in a bath of Krebs-Ringer salt solution. Crypts were lost in the heated regions with a half-time of approximately six hours and reached a steady level of damage by 10--16 hours. There was no recovery in crypt number for one week after hyperthermia. Using a 24 hour assay, crypt survival curves were obtained using various heating times in the temperature range 37.5 degrees C--44.5 degrees C. These curves were qualitatively similar to those resulting from radiation damage, showing a shoulder followed by exponential killing. As the temperature was increased, progressive changes in shape of the curves indicated a proportional inhibition of accumulation of sublethal heat damage combined with increased rate of expression of lethal damage. Over the temperature range 42.3 degrees C--44.5 degrees C, a linear relationship was found between the rate of crypt loss and the reciprocal of the absolute temperature. An activation energy of 600 +/- 70 kJ mole-1 was calculated using the Arrhenius equation. In this temperature range, doubling the heating time had the same effect as increasing the temperature by 1 degree C. At temperatures below about 42.3 degrees C, the tissue became relatively less sensitive to increasing the treatment time.", "contents": "The effect of local hyperthermia on the small intestine of the mouse. Small loops of mouse jejunum were exteriorized and heated by immersion in a bath of Krebs-Ringer salt solution. Crypts were lost in the heated regions with a half-time of approximately six hours and reached a steady level of damage by 10--16 hours. There was no recovery in crypt number for one week after hyperthermia. Using a 24 hour assay, crypt survival curves were obtained using various heating times in the temperature range 37.5 degrees C--44.5 degrees C. These curves were qualitatively similar to those resulting from radiation damage, showing a shoulder followed by exponential killing. As the temperature was increased, progressive changes in shape of the curves indicated a proportional inhibition of accumulation of sublethal heat damage combined with increased rate of expression of lethal damage. Over the temperature range 42.3 degrees C--44.5 degrees C, a linear relationship was found between the rate of crypt loss and the reciprocal of the absolute temperature. An activation energy of 600 +/- 70 kJ mole-1 was calculated using the Arrhenius equation. In this temperature range, doubling the heating time had the same effect as increasing the temperature by 1 degree C. At temperatures below about 42.3 degrees C, the tissue became relatively less sensitive to increasing the treatment time."}
{"id": "PMID:486894", "title": "Recovery from sublethal damage by acutely hypoxic tumour cells in vivo and in vitro.", "content": "The ability of acutely hypoxic tumour cells to recover from sublethal damage following irradiation in vivo and in vitro has been measured using a single tumour system. The methods of assay were tumour growth delay, local tumour control and tumour cell survival in vitro following treatment in vivo or in vitro. Tumours in vivo or cells in vitro rendered acutely hypoxic during irradiation were irradiated with either single doses or two doses 24 hours apart. Cells left in situ had a greater capacity for recovery than those treated either in vivo or in vitro and then assayed in vitro. It is suggested that tumours may not show a systematically reduced capacity for recovery relative to normal tissues, unless chronically hypoxic tumour cells have a reduced capacity for recovery and determine the response. However, the results imply that deductions as to the ability of tumour cells to recover from sublethal damage (whether chronically hypoxic or not), which rely on in vitro assays, may underestimate the extent of recovery.", "contents": "Recovery from sublethal damage by acutely hypoxic tumour cells in vivo and in vitro. The ability of acutely hypoxic tumour cells to recover from sublethal damage following irradiation in vivo and in vitro has been measured using a single tumour system. The methods of assay were tumour growth delay, local tumour control and tumour cell survival in vitro following treatment in vivo or in vitro. Tumours in vivo or cells in vitro rendered acutely hypoxic during irradiation were irradiated with either single doses or two doses 24 hours apart. Cells left in situ had a greater capacity for recovery than those treated either in vivo or in vitro and then assayed in vitro. It is suggested that tumours may not show a systematically reduced capacity for recovery relative to normal tissues, unless chronically hypoxic tumour cells have a reduced capacity for recovery and determine the response. However, the results imply that deductions as to the ability of tumour cells to recover from sublethal damage (whether chronically hypoxic or not), which rely on in vitro assays, may underestimate the extent of recovery."}
{"id": "PMID:486907", "title": "The management of gross refractory obesity by jejuno-ileal bypass.", "content": "Jejuno-ileal bypass has been performed in 226 massively obese patients, 190 of whom have been followed for a minimum of 1 year. End-to-side and end-to-end anastomoses were compared and no difference in the weight reduction achieved by either technique was seen throughout the 5-year follow-up. There was no significant difference in weight reduction achieved between groups of patients in which the jejunal length varied from 10 to 35 cm and the ileal length from 35 to 10 cm. The weight reduction achieved in the entire series averaged 36-7 +/- 9-4 per cent at 2 years, after which no further loss occurred. Good psychological benefits were recorded. Remission of diabetes occurred in 12 of 13 patients and marked long term lowering of serum lipids was seen. Side effects included fluid and electrolyte disturbances and fatty changes in the liver during the first year, improving thereafter. Early cirrhosis of the liver occurred in 8-6 per cent. Other side effects included abdominal bloating, arthralgia and renal colic. The operative mortality was 0-9 per cent and the late mortality directly attributable to the jejuno-ileal bypass, 3-1 per cent. In 19 patients the bypass was discontinued because of severe side effects.", "contents": "The management of gross refractory obesity by jejuno-ileal bypass. Jejuno-ileal bypass has been performed in 226 massively obese patients, 190 of whom have been followed for a minimum of 1 year. End-to-side and end-to-end anastomoses were compared and no difference in the weight reduction achieved by either technique was seen throughout the 5-year follow-up. There was no significant difference in weight reduction achieved between groups of patients in which the jejunal length varied from 10 to 35 cm and the ileal length from 35 to 10 cm. The weight reduction achieved in the entire series averaged 36-7 +/- 9-4 per cent at 2 years, after which no further loss occurred. Good psychological benefits were recorded. Remission of diabetes occurred in 12 of 13 patients and marked long term lowering of serum lipids was seen. Side effects included fluid and electrolyte disturbances and fatty changes in the liver during the first year, improving thereafter. Early cirrhosis of the liver occurred in 8-6 per cent. Other side effects included abdominal bloating, arthralgia and renal colic. The operative mortality was 0-9 per cent and the late mortality directly attributable to the jejuno-ileal bypass, 3-1 per cent. In 19 patients the bypass was discontinued because of severe side effects."}
{"id": "PMID:486908", "title": "Influence of thyroid surgery on voice function and laryngeal symptoms.", "content": "A comprehensive examination of voice function and laryngeal symptoms was applied systematically to 20 patients undergoing thyroid surgery. Patients were excluded from the series if there had been previous neck surgery or if postoperative indirect laryngoscopy showed abnormal cord mobility. Examination before operation showed that patients with sporadic non-toxic goitre or medically pre-treated toxic goitre had impaired voice function based on stroboscopy, electro-glottography, phono-oscillometry and determination of voice range, phonation time, pitch, and peak flow. Three months after thyroid surgery the voice function was significantly improved although not normal in non-toxic goitre patients, whereas the voice function in patients with thyrotoxicosis was unchanged. The number of laryngeal symptoms was significantly reduced in non-toxic goitres following surgery and unaltered in the group with thyrotoxicosis.", "contents": "Influence of thyroid surgery on voice function and laryngeal symptoms. A comprehensive examination of voice function and laryngeal symptoms was applied systematically to 20 patients undergoing thyroid surgery. Patients were excluded from the series if there had been previous neck surgery or if postoperative indirect laryngoscopy showed abnormal cord mobility. Examination before operation showed that patients with sporadic non-toxic goitre or medically pre-treated toxic goitre had impaired voice function based on stroboscopy, electro-glottography, phono-oscillometry and determination of voice range, phonation time, pitch, and peak flow. Three months after thyroid surgery the voice function was significantly improved although not normal in non-toxic goitre patients, whereas the voice function in patients with thyrotoxicosis was unchanged. The number of laryngeal symptoms was significantly reduced in non-toxic goitres following surgery and unaltered in the group with thyrotoxicosis."}
{"id": "PMID:486909", "title": "Lingual thyroid: a review of 12 cases.", "content": "Twelve patients with lingual thyroid are reviewed. Two of these patients had a follicular carcinoma in the ectopic gland and both are well 5 and 16 years after treatment. The diagnosis is essentially clinical but confirmation may be made with a thyroid scan. Six patients were treated conservatively with thyroxine with good results. Six patients were treated by surgical excision, 2 undergoing autotransplantation of the excised gland into the anterior abdominal wall.", "contents": "Lingual thyroid: a review of 12 cases. Twelve patients with lingual thyroid are reviewed. Two of these patients had a follicular carcinoma in the ectopic gland and both are well 5 and 16 years after treatment. The diagnosis is essentially clinical but confirmation may be made with a thyroid scan. Six patients were treated conservatively with thyroxine with good results. Six patients were treated by surgical excision, 2 undergoing autotransplantation of the excised gland into the anterior abdominal wall."}
{"id": "PMID:486912", "title": "Postprandial serum gastrin levels in patients with combined hypergastrinaemia and hyperchlorhydria.", "content": "We have determined the serum gastrin response to a standard test meal in 13 unoperated patients with combined fasting hypergastrinaemia (more than 150 ng/l) and basal hypersecretion of gastric acid (BAO more than 10 mmol/h). In 10 of 11 patients with proved of presumptive Zollinger-Ellison syndrome the peak postprandial rise in serum gastrin concentration was less than 50 per cent of basal value. The integrated gastrin response to feeding ranged from 71-9 to 211-8 mug/lX 150 min. In 2 patients with proved hypergastrinaemia of antral origin, however, serum gastrin rose to 223 per cent and 255 per cent respectively of basal value after ingestion of the meal. The integrated postprandial serum gastrin responses in these patients were 66-8 and 22-0 mug/lX 150 min. Two patiets with Zollinger-Ellison syndrome and total gastrectomy showed peak serum gastrin levels after feeding of 174 and 255 per cent of basal concentration. The integrated postprandial gastrin secretions were 365 and 366 mug/l X 150 min respectively. It is concluded that the serum gastrin response to feeding, when expressed as percentage change, may be helpful in the differential diagnosis of unoperated patients with fasting hypergastrinaemia and basal gastric acid hypersecretion.", "contents": "Postprandial serum gastrin levels in patients with combined hypergastrinaemia and hyperchlorhydria. We have determined the serum gastrin response to a standard test meal in 13 unoperated patients with combined fasting hypergastrinaemia (more than 150 ng/l) and basal hypersecretion of gastric acid (BAO more than 10 mmol/h). In 10 of 11 patients with proved of presumptive Zollinger-Ellison syndrome the peak postprandial rise in serum gastrin concentration was less than 50 per cent of basal value. The integrated gastrin response to feeding ranged from 71-9 to 211-8 mug/lX 150 min. In 2 patients with proved hypergastrinaemia of antral origin, however, serum gastrin rose to 223 per cent and 255 per cent respectively of basal value after ingestion of the meal. The integrated postprandial serum gastrin responses in these patients were 66-8 and 22-0 mug/lX 150 min. Two patiets with Zollinger-Ellison syndrome and total gastrectomy showed peak serum gastrin levels after feeding of 174 and 255 per cent of basal concentration. The integrated postprandial gastrin secretions were 365 and 366 mug/l X 150 min respectively. It is concluded that the serum gastrin response to feeding, when expressed as percentage change, may be helpful in the differential diagnosis of unoperated patients with fasting hypergastrinaemia and basal gastric acid hypersecretion."}
{"id": "PMID:486911", "title": "Effect of epidural analgesia on metabolic profiles during and after surgery.", "content": "Blood concentrations of glucose, lactate, alanine, free fatty acids, glycerol and 3-hydroxybutyrate were measured during 24 h in 12 females undergoing elective hysterectomy under either general anaesthesia or epidural analgesia extending from T4 to S5. The results showed that the peroperative and immediate postoperative metabolic profiles in the group receiving general anaesthesia reflected substrate mobilization (increased glucose, lactate, 3-hydroxybutyrate), and that epidural analgesia blocked these changes, probably mediated through an inhibited adrenaline response to surgery. Peroperative values of free fatty acids and glycerol were significantly lowered by epidural analgesia, indicating inhibition of lipolysis. Alanine decreased similarly in both groups. In the later postoperative period metabolic profiles were similar in two groups, indicating that the influence of fasting may override the effect of the relatively minor trauma.", "contents": "Effect of epidural analgesia on metabolic profiles during and after surgery. Blood concentrations of glucose, lactate, alanine, free fatty acids, glycerol and 3-hydroxybutyrate were measured during 24 h in 12 females undergoing elective hysterectomy under either general anaesthesia or epidural analgesia extending from T4 to S5. The results showed that the peroperative and immediate postoperative metabolic profiles in the group receiving general anaesthesia reflected substrate mobilization (increased glucose, lactate, 3-hydroxybutyrate), and that epidural analgesia blocked these changes, probably mediated through an inhibited adrenaline response to surgery. Peroperative values of free fatty acids and glycerol were significantly lowered by epidural analgesia, indicating inhibition of lipolysis. Alanine decreased similarly in both groups. In the later postoperative period metabolic profiles were similar in two groups, indicating that the influence of fasting may override the effect of the relatively minor trauma."}
{"id": "PMID:486913", "title": "Prolonged post-vagotomy gastric atony treated by oxytocin.", "content": "Persistent and complete post-vagotomy gastric atony was treated in 3 patients by intravenous infusion of oxytocin. Despite failure of both conventional treatment with gastric aspiration and intravenous fluids or jejunal feeding, as well as the reported trials with bethanechol chloride and metaclopramide, these patients promptly responded to oxytocin. It appears that the latter may represent a solution to this uncommon but recalcitrant complication of peptic ulcer surgery.", "contents": "Prolonged post-vagotomy gastric atony treated by oxytocin. Persistent and complete post-vagotomy gastric atony was treated in 3 patients by intravenous infusion of oxytocin. Despite failure of both conventional treatment with gastric aspiration and intravenous fluids or jejunal feeding, as well as the reported trials with bethanechol chloride and metaclopramide, these patients promptly responded to oxytocin. It appears that the latter may represent a solution to this uncommon but recalcitrant complication of peptic ulcer surgery."}
{"id": "PMID:486915", "title": "Tracheal collapse after thyroidectomy.", "content": "Tracheal collapse after thyroidectomy is uncommon; when it occurs, complete airway obstruction is often sudden, unexpected and fatal. In this paper a patient treated for tracheal collapse by tracheostomy and followed up for 1 year is described and, from a review of the literature, a further 9 cases are presented. The aetiology of the condition, the patient at risk and the technique of management are discussed.", "contents": "Tracheal collapse after thyroidectomy. Tracheal collapse after thyroidectomy is uncommon; when it occurs, complete airway obstruction is often sudden, unexpected and fatal. In this paper a patient treated for tracheal collapse by tracheostomy and followed up for 1 year is described and, from a review of the literature, a further 9 cases are presented. The aetiology of the condition, the patient at risk and the technique of management are discussed."}
{"id": "PMID:486914", "title": "Autoparathyroidectomy: a case report.", "content": "This report describes a patient with primary hyperparathyroidism who became spontaneously normocalcaemic preoperatively. This was due to infarction in a parathyroid adenoma. Plasma parathormone (PTH) levels were monitored pre- and postoperatively.", "contents": "Autoparathyroidectomy: a case report. This report describes a patient with primary hyperparathyroidism who became spontaneously normocalcaemic preoperatively. This was due to infarction in a parathyroid adenoma. Plasma parathormone (PTH) levels were monitored pre- and postoperatively."}
{"id": "PMID:486917", "title": "Experiences with head injuries in a regional neurosurgical unit.", "content": "A review of all patients with major head injuries transferred to a regional neurosurgical unit for the years 1975 and 1976 has been carried out for comparison with the total number of patients with head injury admitted to district general hospitals of the region. Fifty-four per cent of patients admitted to the neurosurgical unit have resumed their old occupation and 20 per cent died. The significance of these figures is discussed, particularly in the light of the need for a more comprehensive policy for treating head injuries within the United Kingdom.", "contents": "Experiences with head injuries in a regional neurosurgical unit. A review of all patients with major head injuries transferred to a regional neurosurgical unit for the years 1975 and 1976 has been carried out for comparison with the total number of patients with head injury admitted to district general hospitals of the region. Fifty-four per cent of patients admitted to the neurosurgical unit have resumed their old occupation and 20 per cent died. The significance of these figures is discussed, particularly in the light of the need for a more comprehensive policy for treating head injuries within the United Kingdom."}
{"id": "PMID:486916", "title": "Carcinoma of the thyroid.", "content": "Total thyroidectomy with excision of any enlarged cervical lymph nodes was the usual operation practised for carcinoma of the thyroid in this series. Extended thyroidectomy afforded cures in some patients with locally advanced disease. Preoperative drill biopsy of the goitre was a useful procedure. Other noteworthy features were the increasing occurrence of papillary carcinoma and the comparatively high incidence of malignancy in solitary nodules.", "contents": "Carcinoma of the thyroid. Total thyroidectomy with excision of any enlarged cervical lymph nodes was the usual operation practised for carcinoma of the thyroid in this series. Extended thyroidectomy afforded cures in some patients with locally advanced disease. Preoperative drill biopsy of the goitre was a useful procedure. Other noteworthy features were the increasing occurrence of papillary carcinoma and the comparatively high incidence of malignancy in solitary nodules."}
{"id": "PMID:486919", "title": "Pyloric diaphragm, antral web, congenital antral membrane--a surgery rarity?", "content": "Three cases of pyloric diaphragm in elderly patients are reported. The finding of these patients within a 2-year period (1976-8) suggests that the condition is not as rare as previously thought. Diagnosis was achieved endoscopically and the condition managed surgically. While there has been controversy in the past over whether the disorder is acquired or congenital, the histological evidence of the cases presented here tends to suggest that it is a late presentation of a congenital abnormality.", "contents": "Pyloric diaphragm, antral web, congenital antral membrane--a surgery rarity? Three cases of pyloric diaphragm in elderly patients are reported. The finding of these patients within a 2-year period (1976-8) suggests that the condition is not as rare as previously thought. Diagnosis was achieved endoscopically and the condition managed surgically. While there has been controversy in the past over whether the disorder is acquired or congenital, the histological evidence of the cases presented here tends to suggest that it is a late presentation of a congenital abnormality."}
{"id": "PMID:486918", "title": "Experience in the management of 50 consecutive penetrating wounds of the heart.", "content": "Fifty patients with penetrating wounds of the heart were grouped into three categories, based on the clinical severity of the injuries. Gunshot wounds consistently resulted in a higher mortality than knife wounds. Central venous pressure (CVP) was the single most important means of determining cardiac tamponade. The survival rates in the respective groups were 6, 79 and 100 per cent. The improved survival in our series was due to a rapid transportation system, increased awareness of the significance of cardiac injuries and an aggressive surgical approach.", "contents": "Experience in the management of 50 consecutive penetrating wounds of the heart. Fifty patients with penetrating wounds of the heart were grouped into three categories, based on the clinical severity of the injuries. Gunshot wounds consistently resulted in a higher mortality than knife wounds. Central venous pressure (CVP) was the single most important means of determining cardiac tamponade. The survival rates in the respective groups were 6, 79 and 100 per cent. The improved survival in our series was due to a rapid transportation system, increased awareness of the significance of cardiac injuries and an aggressive surgical approach."}
{"id": "PMID:486921", "title": "Ingrowing toenails: the results of treatment.", "content": "Completed questionnaires were received from 208 patients on whom 245 operations for ingrowing toenails had been performed at King's College Hospital. Avulsion of a strip of nail had an 83 per cent recurrence rate, total nail avulsion 70 per cent, Zadik operations 28 per cent and wedge resections 29 per cent. It is recommended that immediate avulsion of ingrown toenails should not be a routine practice.", "contents": "Ingrowing toenails: the results of treatment. Completed questionnaires were received from 208 patients on whom 245 operations for ingrowing toenails had been performed at King's College Hospital. Avulsion of a strip of nail had an 83 per cent recurrence rate, total nail avulsion 70 per cent, Zadik operations 28 per cent and wedge resections 29 per cent. It is recommended that immediate avulsion of ingrown toenails should not be a routine practice."}
{"id": "PMID:486923", "title": "Solitary toxic adenoma of the thyroid gland.", "content": "The incidence of solitary toxic adenoma of the thyroid in a general surgical unit with an interest in thyroid disease has been reviewed over a 15-year period. Six hundred and thirty thyrotoxic cases were treated surgically, 35 (5.6 per cent) having a solitary toxic adenoma. Thyroid enlargement or toxicity had been present for more than 5 years in 7 patients (20.0 per cent). Cardiovascular complications were present in 6 cases (17.1 per cent). Thyroid lobectomy resulted in 30 (85.7 per cent) euthyroid and 5 (143.3 per cent) hypothyroid patients. One toxic adenoma contained a focus of carcinoma. The clinical features, diagnosis and management of solitary toxic adenoma, and the management of symptomatic nodules which are 'hot' but not biochemically toxic, are discussed.", "contents": "Solitary toxic adenoma of the thyroid gland. The incidence of solitary toxic adenoma of the thyroid in a general surgical unit with an interest in thyroid disease has been reviewed over a 15-year period. Six hundred and thirty thyrotoxic cases were treated surgically, 35 (5.6 per cent) having a solitary toxic adenoma. Thyroid enlargement or toxicity had been present for more than 5 years in 7 patients (20.0 per cent). Cardiovascular complications were present in 6 cases (17.1 per cent). Thyroid lobectomy resulted in 30 (85.7 per cent) euthyroid and 5 (143.3 per cent) hypothyroid patients. One toxic adenoma contained a focus of carcinoma. The clinical features, diagnosis and management of solitary toxic adenoma, and the management of symptomatic nodules which are 'hot' but not biochemically toxic, are discussed."}
{"id": "PMID:486924", "title": "A case of craniocleidal dysostosis presenting with vascular complications.", "content": "A case of craniocleidal dysostosis was presented. In spite of the skeletal abnormalities present, the disease was not diagnosed until the patient, a married woman of 63, developed ischaemia of her right upper limb, followed by cerebral symptoms. An arch aortogram demonstrated an occlusion of the first part of the right subclavian artery involving the origin of the vertebral artery and a post-stenotic dilatation of the left subclavian artery due to costoclavicular compression. Such vascular abnormalities have not been previously described in this condition.", "contents": "A case of craniocleidal dysostosis presenting with vascular complications. A case of craniocleidal dysostosis was presented. In spite of the skeletal abnormalities present, the disease was not diagnosed until the patient, a married woman of 63, developed ischaemia of her right upper limb, followed by cerebral symptoms. An arch aortogram demonstrated an occlusion of the first part of the right subclavian artery involving the origin of the vertebral artery and a post-stenotic dilatation of the left subclavian artery due to costoclavicular compression. Such vascular abnormalities have not been previously described in this condition."}
{"id": "PMID:486931", "title": "Effects of stopping smoking for 48 hours on oxygen availability from the blood: a study on pregnant women.", "content": "The effects of stopping smoking for 48 hours on factors governing the availability of oxygen from the blood--that is, carboxyhaemoglobin (COHb), haemoglobin-oxygen (HbO2) affinity, and haemoglobin concentration--were measured in women in the last trimester of pregnancy. Three groups were studied: smokers, smokers who stopped smoking for 48 hours, and non-smokers. The 22 smokers had higher initial COHb values and greater HbO2 affinity than the 10 non-smokers, but their total haemoglobin concentrations were also higher, so that their oxygen availability was not significantly reduced. In the 11 smokers who stopped the reduction in COHb and decrease in HbO2 affinity led to a significant increase of 8% in \"available oxygen\" in 48 hours. Since even small improvements in oxygen delivery to the tissues may confer critical benefit to the fetus, particularly during labour or when exposed to general anaesthesia, smoking should be discouraged for 48 hours before elective deliveries. The same consideration might reasonably be applied to patients undergoing general anaesthesia for all elective operations.", "contents": "Effects of stopping smoking for 48 hours on oxygen availability from the blood: a study on pregnant women. The effects of stopping smoking for 48 hours on factors governing the availability of oxygen from the blood--that is, carboxyhaemoglobin (COHb), haemoglobin-oxygen (HbO2) affinity, and haemoglobin concentration--were measured in women in the last trimester of pregnancy. Three groups were studied: smokers, smokers who stopped smoking for 48 hours, and non-smokers. The 22 smokers had higher initial COHb values and greater HbO2 affinity than the 10 non-smokers, but their total haemoglobin concentrations were also higher, so that their oxygen availability was not significantly reduced. In the 11 smokers who stopped the reduction in COHb and decrease in HbO2 affinity led to a significant increase of 8% in \"available oxygen\" in 48 hours. Since even small improvements in oxygen delivery to the tissues may confer critical benefit to the fetus, particularly during labour or when exposed to general anaesthesia, smoking should be discouraged for 48 hours before elective deliveries. The same consideration might reasonably be applied to patients undergoing general anaesthesia for all elective operations."}
{"id": "PMID:486932", "title": "Self-titration by cigarette smokers.", "content": "An 11-week crossover study was carried out in which 12 subjects smoked high-nicotine (1.84 mg standard yield) and low-nicotine (0.6 mg) cigarettes after an initial period of smoking their usual brands with a medium-nicotine yield (mean 1.4 mg). Plasma and urine nicotine concentrations, carboxyhaemoglobin (COHb) concentration, puffing behaviour, 24-hour cigarette consumption, and butt nicotine content were measured. The changes in plasma nicotine and blood COHb concentrations showed that the smokers compensated for about two-thirds of the difference in standard yields when switched to either high- or low-nicotine cigarettes. Thus, compared with the medium-nicotine brand, the intake of nicotine and carbon monoxide was only about 10% higher when subjects smoked the high-nicotine cigarettes, which had a standard yield 30-40% higher than the medium brands; and only about 15% lower when they smoked the low-nicotine cigarettes, which had a standard yield about 50% lower than the medium brands. But nicotine content and urine nicotine concentrations followed a similar pattern. Changes in puffing behaviour and in 24-hour cigarette consumption were only slight.The results show clear evidence of both upward and downward self-titration of nicotine and carbon monoxide (and tar) intakes when smokers change to cigarettes with standard yields that differ over the range studied.", "contents": "Self-titration by cigarette smokers. An 11-week crossover study was carried out in which 12 subjects smoked high-nicotine (1.84 mg standard yield) and low-nicotine (0.6 mg) cigarettes after an initial period of smoking their usual brands with a medium-nicotine yield (mean 1.4 mg). Plasma and urine nicotine concentrations, carboxyhaemoglobin (COHb) concentration, puffing behaviour, 24-hour cigarette consumption, and butt nicotine content were measured. The changes in plasma nicotine and blood COHb concentrations showed that the smokers compensated for about two-thirds of the difference in standard yields when switched to either high- or low-nicotine cigarettes. Thus, compared with the medium-nicotine brand, the intake of nicotine and carbon monoxide was only about 10% higher when subjects smoked the high-nicotine cigarettes, which had a standard yield 30-40% higher than the medium brands; and only about 15% lower when they smoked the low-nicotine cigarettes, which had a standard yield about 50% lower than the medium brands. But nicotine content and urine nicotine concentrations followed a similar pattern. Changes in puffing behaviour and in 24-hour cigarette consumption were only slight.The results show clear evidence of both upward and downward self-titration of nicotine and carbon monoxide (and tar) intakes when smokers change to cigarettes with standard yields that differ over the range studied."}
{"id": "PMID:486933", "title": "Adverse reactions to frusemide in hospital inpatients.", "content": "Out of 2580 medical inpatients included in a drug-surveillance programme, 585 (22.7%) were treated with frusemide. Of these, 123 (21.0%) had a total of 177 adverse reactions. The most common were hypovolaemia (85 cases), hyperuricaemia (54), and hypokalaemia (21). Most reactions were mild, and only three patients had potentially life-threatening effects. The incidence of adverse reactions increased significantly with daily dose, occurring in 47 patients (13.5%) given up to 40 mg, 42 (26.3%) given up to 80 mg, and 34 (43.6%) given over 80 mg (P less than 0.001). There was no clear association between side effects and a raised blood urea concentration on admission, confirming that treatment with frusemide is not more hazardous in patients with renal failure. Frusemide is a safe and highly effective diuretic. Nevertheless, in view of the potential seriousness of volume depletion, dosage should probably begin at 20 rather than 40 mg daily.", "contents": "Adverse reactions to frusemide in hospital inpatients. Out of 2580 medical inpatients included in a drug-surveillance programme, 585 (22.7%) were treated with frusemide. Of these, 123 (21.0%) had a total of 177 adverse reactions. The most common were hypovolaemia (85 cases), hyperuricaemia (54), and hypokalaemia (21). Most reactions were mild, and only three patients had potentially life-threatening effects. The incidence of adverse reactions increased significantly with daily dose, occurring in 47 patients (13.5%) given up to 40 mg, 42 (26.3%) given up to 80 mg, and 34 (43.6%) given over 80 mg (P less than 0.001). There was no clear association between side effects and a raised blood urea concentration on admission, confirming that treatment with frusemide is not more hazardous in patients with renal failure. Frusemide is a safe and highly effective diuretic. Nevertheless, in view of the potential seriousness of volume depletion, dosage should probably begin at 20 rather than 40 mg daily."}
{"id": "PMID:486966", "title": "Psychosocial stress in pregnancy and its relation to the onset of premature labour.", "content": "A modified life events inventory was presented over a four-month period to 132 consecutive women going into spontaneous labour in Hull and Manchester. Three study groups were identified according to the duration of pregnancy. The levels of psychosocial stress in pregnancy were found to be particularly high in the mothers whose babies were born preterm. Stressful events may precipitate preterm labour in some women. The concept of antenatal care may have to be broadened if the incidence of premature labour and resulting perinatal mortality are to be reduced.", "contents": "Psychosocial stress in pregnancy and its relation to the onset of premature labour. A modified life events inventory was presented over a four-month period to 132 consecutive women going into spontaneous labour in Hull and Manchester. Three study groups were identified according to the duration of pregnancy. The levels of psychosocial stress in pregnancy were found to be particularly high in the mothers whose babies were born preterm. Stressful events may precipitate preterm labour in some women. The concept of antenatal care may have to be broadened if the incidence of premature labour and resulting perinatal mortality are to be reduced."}
{"id": "PMID:486968", "title": "Evaluation of the forced expiration technique as an adjunct to postural drainage in treatment of cystic fibrosis.", "content": "Sixteen patients with cystic fibrosis were treated with conventional physiotherapy aided by an assistant. The results were compared with those produced by physiotherapy using the forced expiration technique cleared more sputum in less time than conventional physiotherapy. A sputum in less time than conventional physiotherapy. A second study showed that an assistant did not further improve the results obtained by the patient performing the forced expiration technique himself. These findings mean that patients with cystic fibrosis who have had to rely on the help of others for their home treatment may now perform more effective treatment without help. The forced expiration technique might also be helpful for patients with chronic bronchitis, asthma, or bronchiectasis.", "contents": "Evaluation of the forced expiration technique as an adjunct to postural drainage in treatment of cystic fibrosis. Sixteen patients with cystic fibrosis were treated with conventional physiotherapy aided by an assistant. The results were compared with those produced by physiotherapy using the forced expiration technique cleared more sputum in less time than conventional physiotherapy. A sputum in less time than conventional physiotherapy. A second study showed that an assistant did not further improve the results obtained by the patient performing the forced expiration technique himself. These findings mean that patients with cystic fibrosis who have had to rely on the help of others for their home treatment may now perform more effective treatment without help. The forced expiration technique might also be helpful for patients with chronic bronchitis, asthma, or bronchiectasis."}
{"id": "PMID:486967", "title": "Referral of mothers and infants for intensive care.", "content": "During 1975-7, 96 mothers were referred to University College Hospital for delivery from 39 other hospitals because their pregnancies were considered to be at very high risk. One hundred of the 111 infants born to the 96 mothers weighed 2500 g or less and 60 weighed 1500 g or less. A high proportion of the infants developed serious illnesses necessitating intensive care. The birth-weight-specific neonatal mortality rates of the infants were much lower than those of infants born in England and Wales as a whole and were also lower than those of the 370 infants transported to this hospital for intensive care after delivery elsewhere. Whenever possible mothers with very high-risk pregnancies should be referred for delivery to centres with full facilities for the intensive care of the mother, fetus, and newborn infant.", "contents": "Referral of mothers and infants for intensive care. During 1975-7, 96 mothers were referred to University College Hospital for delivery from 39 other hospitals because their pregnancies were considered to be at very high risk. One hundred of the 111 infants born to the 96 mothers weighed 2500 g or less and 60 weighed 1500 g or less. A high proportion of the infants developed serious illnesses necessitating intensive care. The birth-weight-specific neonatal mortality rates of the infants were much lower than those of infants born in England and Wales as a whole and were also lower than those of the 370 infants transported to this hospital for intensive care after delivery elsewhere. Whenever possible mothers with very high-risk pregnancies should be referred for delivery to centres with full facilities for the intensive care of the mother, fetus, and newborn infant."}
{"id": "PMID:486969", "title": "Are increased individual susceptibility and environmental factors both necessary for the development of primary biliary cirrhosis?", "content": "A daughter, her mother, and an unrelated close friend developed primary biliary cirrhosis (PBC). The mother and the close friend nursed the daughter through her terminal illness and presented with PBC PBC within 21 months after her death. Half of the asymptomatic first-degree relatives in the two families had serum autoantibodies, including one with antimitochondrial antibody, suggesting some genetic susceptibility to abnormal immune reactions. It is concluded that some environmental factor may be present in PBC, perhaps in addition to a genetic susceptibility to that factor.", "contents": "Are increased individual susceptibility and environmental factors both necessary for the development of primary biliary cirrhosis? A daughter, her mother, and an unrelated close friend developed primary biliary cirrhosis (PBC). The mother and the close friend nursed the daughter through her terminal illness and presented with PBC PBC within 21 months after her death. Half of the asymptomatic first-degree relatives in the two families had serum autoantibodies, including one with antimitochondrial antibody, suggesting some genetic susceptibility to abnormal immune reactions. It is concluded that some environmental factor may be present in PBC, perhaps in addition to a genetic susceptibility to that factor."}
{"id": "PMID:487004", "title": "Meningococcal infections during infancy: confidential inquiries into 10 deaths.", "content": "The first 10 deaths from meningococcal infections in children aged under 2 years that were reported to a DHSS multicentre study were reviewed. Confidential inquiries were made of the parents, family doctors, health visitors, and hospital staff concerned with each case, and management was discussed with a paediatrician and pathologist. Diagnosis and treatment were often delayed because doctors did not realise the importance of the purpuric rash. One child died at home, and by the time they were admitted to hospital all the remaining nine were shocked and needed resuscitation. Prodromal symptoms, mainly changes in behaviour, preceded the rash in all cases. These prodromal symptoms should arouse the suspicion of septicaemia and prompt a search for petechiae so that early effective treatment may be started.", "contents": "Meningococcal infections during infancy: confidential inquiries into 10 deaths. The first 10 deaths from meningococcal infections in children aged under 2 years that were reported to a DHSS multicentre study were reviewed. Confidential inquiries were made of the parents, family doctors, health visitors, and hospital staff concerned with each case, and management was discussed with a paediatrician and pathologist. Diagnosis and treatment were often delayed because doctors did not realise the importance of the purpuric rash. One child died at home, and by the time they were admitted to hospital all the remaining nine were shocked and needed resuscitation. Prodromal symptoms, mainly changes in behaviour, preceded the rash in all cases. These prodromal symptoms should arouse the suspicion of septicaemia and prompt a search for petechiae so that early effective treatment may be started."}
{"id": "PMID:487003", "title": "A unit for source and protective isolation in a general hospital.", "content": "An isolation unit consisting of 12 ventilated cubicles was investigated over 18 months. Out of 462 patients admitted, 262 (57%) required source and 200 (43%) protective isolation. Admissions of patients with staphylococcal sepsis fell from 16 in the first three months to six in the last three months. Staphylococcus aureus was recovered from 12% of nurses' fingers and often in small numbers from protective clothing and uniforms, but only two patients acquired a strain from a nurse or another patient. Gram-negative bacilli were rarely recovered from hands or protective clothing of nurses, and there was no evidence of spread of infectious diseases. This inexpensive unit, with simple but efficient isolation-nursing techniques, successfully prevented the spread of infection.", "contents": "A unit for source and protective isolation in a general hospital. An isolation unit consisting of 12 ventilated cubicles was investigated over 18 months. Out of 462 patients admitted, 262 (57%) required source and 200 (43%) protective isolation. Admissions of patients with staphylococcal sepsis fell from 16 in the first three months to six in the last three months. Staphylococcus aureus was recovered from 12% of nurses' fingers and often in small numbers from protective clothing and uniforms, but only two patients acquired a strain from a nurse or another patient. Gram-negative bacilli were rarely recovered from hands or protective clothing of nurses, and there was no evidence of spread of infectious diseases. This inexpensive unit, with simple but efficient isolation-nursing techniques, successfully prevented the spread of infection."}
{"id": "PMID:487005", "title": "Alcoholism in the general hospital.", "content": "To assess the prevalence of alcoholism among people admitted to hospital 303 patients completed a drinking questionnaire. A total of 59 (19.5%) were found to have a drinking problem, which constituted a sixfold greater prevalence than recorded in a community survey using the same technique. The drinkers were mostly men and tended to be younger than the non-drinkers and to smoke more heavily, live in more crowded conditions, and be of lower social class. Significantly more of the drinkers had at least one parent who was an alcoholic. The results confirm that hospital inpatients comprise a larger proportion of alcoholics than found in the general population. Hence medical staff should be alert to such patients, so that treatment may be initiated at an early stage of social decompensation.", "contents": "Alcoholism in the general hospital. To assess the prevalence of alcoholism among people admitted to hospital 303 patients completed a drinking questionnaire. A total of 59 (19.5%) were found to have a drinking problem, which constituted a sixfold greater prevalence than recorded in a community survey using the same technique. The drinkers were mostly men and tended to be younger than the non-drinkers and to smoke more heavily, live in more crowded conditions, and be of lower social class. Significantly more of the drinkers had at least one parent who was an alcoholic. The results confirm that hospital inpatients comprise a larger proportion of alcoholics than found in the general population. Hence medical staff should be alert to such patients, so that treatment may be initiated at an early stage of social decompensation."}
{"id": "PMID:487063", "title": "Optokinetic nystagmus and the accessory optic system of pigeon and turtle.", "content": "Optokinetic nystagmus (OKN) response functions were obtained in pigeon (Columba livia) and turtle (Chrysemys picta) before and after electrolytic lesions of the accessory optic nuclei (AON). Postlesion retinal input to the AON was evaluated using standard autoradiographic techniques. Bilateral destruction of AON in both pigeon and turtle did not abolish OKN, but was correlated instead with a reduction in OKN frequencies at high pattern velocities. A difference was observed between species with respect to the effects of partial lesions. Incomplete destruction of AON produced no observable change in OKN response functions in pigeon, but correlated with reduced OKN response functions in turtle. These results suggest that the AON mediate a portion of OKN in both pigeon and turtle, particularly at high pattern velocities, but are not essential for its occurrence.", "contents": "Optokinetic nystagmus and the accessory optic system of pigeon and turtle. Optokinetic nystagmus (OKN) response functions were obtained in pigeon (Columba livia) and turtle (Chrysemys picta) before and after electrolytic lesions of the accessory optic nuclei (AON). Postlesion retinal input to the AON was evaluated using standard autoradiographic techniques. Bilateral destruction of AON in both pigeon and turtle did not abolish OKN, but was correlated instead with a reduction in OKN frequencies at high pattern velocities. A difference was observed between species with respect to the effects of partial lesions. Incomplete destruction of AON produced no observable change in OKN response functions in pigeon, but correlated with reduced OKN response functions in turtle. These results suggest that the AON mediate a portion of OKN in both pigeon and turtle, particularly at high pattern velocities, but are not essential for its occurrence."}
{"id": "PMID:487064", "title": "Seaward orientation of hatchling turtles: turning systems in the optic tectum.", "content": "This paper reports studies on the mechanisms underlying seaward orientation in hatchling turtles. The particular aim was to investigate whether activity in different regions of the retina and associated tectal areas, as assessed by some comparator mechanism, results in turning in different directions. Hatchling green turtles (Chelonia mydas) were tested for sea-finding ability in a natural situation on the beach following lesions of the optic tectum. Asymmetrical bilateral lesions resulted in a number of turtles making circles in the direction of the tectum with the posterior lesion and in other turtles deviated in this direction. Bilaterally lesioned turtles were also slower and less consistent in their sea-finding behaviour. No major disruptions of sea finding were detected in animals with unilateral tectal lesions. By suspending lights in the nasal visual field of unilaterally blindfolded green and leatherback turtles (Dermochelys coriacea) it was possible to produce circling in the direction of the covered eye; in contrast, with the light suspended in the temporal field, turning was always in the direction of the uncovered eye. The results are consistent with the view that sea finding depends on a complex phototropotactic system with stimulation in different parts of a single retina being associated with turning in opposite directions.", "contents": "Seaward orientation of hatchling turtles: turning systems in the optic tectum. This paper reports studies on the mechanisms underlying seaward orientation in hatchling turtles. The particular aim was to investigate whether activity in different regions of the retina and associated tectal areas, as assessed by some comparator mechanism, results in turning in different directions. Hatchling green turtles (Chelonia mydas) were tested for sea-finding ability in a natural situation on the beach following lesions of the optic tectum. Asymmetrical bilateral lesions resulted in a number of turtles making circles in the direction of the tectum with the posterior lesion and in other turtles deviated in this direction. Bilaterally lesioned turtles were also slower and less consistent in their sea-finding behaviour. No major disruptions of sea finding were detected in animals with unilateral tectal lesions. By suspending lights in the nasal visual field of unilaterally blindfolded green and leatherback turtles (Dermochelys coriacea) it was possible to produce circling in the direction of the covered eye; in contrast, with the light suspended in the temporal field, turning was always in the direction of the uncovered eye. The results are consistent with the view that sea finding depends on a complex phototropotactic system with stimulation in different parts of a single retina being associated with turning in opposite directions."}
{"id": "PMID:487065", "title": "Brain weight-body weight scaling in breeds of dogs and cats.", "content": "Autopsy data from 2,100 pet dogs are used to demonstrate that mean brain weight (y) in 26 breeds of dogs is related to mean body weight (x) by the function y = 0.39 x 0.27. Power functions with the exponent 0.27 appear to define intra-species brain-body weight scaling in several other species. These functions may serve as base lines for measuring increases or decreases in encephalization during species evolution. Curiously, averaged data from 1,250 domestic cats of 2 breeds can be plotted on a line with slope of 0.67, which is known to define inter- rather than intra-species brain-body weight scaling among mammals.", "contents": "Brain weight-body weight scaling in breeds of dogs and cats. Autopsy data from 2,100 pet dogs are used to demonstrate that mean brain weight (y) in 26 breeds of dogs is related to mean body weight (x) by the function y = 0.39 x 0.27. Power functions with the exponent 0.27 appear to define intra-species brain-body weight scaling in several other species. These functions may serve as base lines for measuring increases or decreases in encephalization during species evolution. Curiously, averaged data from 1,250 domestic cats of 2 breeds can be plotted on a line with slope of 0.67, which is known to define inter- rather than intra-species brain-body weight scaling among mammals."}
{"id": "PMID:487074", "title": "Indented synapses in Aplysia.", "content": "A new type of synaptic contact has been found in Aplysia californica, in which a post-synaptic spine extensively invaginates the pre-synaptic element. The post-synaptic spine, usually less than 0.25 micrometer in diameter, may protrude up to 2 micrometer into the pre-synaptic element. In some instances a larger post-synaptic element indents and forms multiple thin projections into the pre-synaptic varicosity. Along or at the end of these projections a zone occurs at which the surface membranes of the two apposed synaptic elements are rigidly parallel, and the extracellular gap is approximately 60% greater than normal and contains a small amount of electron-dense material. Synaptic vesicles are concentrated against the pre-synaptic membrane in these regions. There are twice as many vesicles per unit area positioned against the membrane at these zones than at similar active zones occurring in the alternative type of synapse, which has a flat, rather than indented, geometry. Single pre-synaptic varicosities have been found to form both flat and indented synapses. These findings raise the possibility that these two forms of synapse may be dynamic transformations of each other, having differing synaptic effectiveness.", "contents": "Indented synapses in Aplysia. A new type of synaptic contact has been found in Aplysia californica, in which a post-synaptic spine extensively invaginates the pre-synaptic element. The post-synaptic spine, usually less than 0.25 micrometer in diameter, may protrude up to 2 micrometer into the pre-synaptic element. In some instances a larger post-synaptic element indents and forms multiple thin projections into the pre-synaptic varicosity. Along or at the end of these projections a zone occurs at which the surface membranes of the two apposed synaptic elements are rigidly parallel, and the extracellular gap is approximately 60% greater than normal and contains a small amount of electron-dense material. Synaptic vesicles are concentrated against the pre-synaptic membrane in these regions. There are twice as many vesicles per unit area positioned against the membrane at these zones than at similar active zones occurring in the alternative type of synapse, which has a flat, rather than indented, geometry. Single pre-synaptic varicosities have been found to form both flat and indented synapses. These findings raise the possibility that these two forms of synapse may be dynamic transformations of each other, having differing synaptic effectiveness."}
{"id": "PMID:487073", "title": "Effect of polyamines on cell-free protein synthesizing systems from rat cerebral cortex, cerebellum and liver.", "content": "The naturally occurring polyamines spermine and spermidine stimulate in low concentrations the amino acid-incorporating activity of cell-free systems from cerebral cortex, cerebellum and liver from the rat to a significant degree, but in high concentrations they have strong inhibiting effects on these systems. Maximal polyamine stimulation is observed with suboptimal Mg2+ concentrations in the reaction mixtures; increasing Mg2+ to 15 mM results in an inhibition of cell-free protein synthesis by polyamines even at low concentrations. The stimulating effect of spermine and spermidine involves a facilitated binding of template RNA and aminoacylated tRNA to the ribosomes. In contrast, the aminoacylation of tRNA is not influenced. These data indicate that spermine as well as spermidine have regulatory functions during the translational process in cerebral cortex, cerebellum and liver. They seem to interact with ribosomal functions and to cause a shift in the optimal Mg2+ concentration required for the synthesis of brain and liver proteins.", "contents": "Effect of polyamines on cell-free protein synthesizing systems from rat cerebral cortex, cerebellum and liver. The naturally occurring polyamines spermine and spermidine stimulate in low concentrations the amino acid-incorporating activity of cell-free systems from cerebral cortex, cerebellum and liver from the rat to a significant degree, but in high concentrations they have strong inhibiting effects on these systems. Maximal polyamine stimulation is observed with suboptimal Mg2+ concentrations in the reaction mixtures; increasing Mg2+ to 15 mM results in an inhibition of cell-free protein synthesis by polyamines even at low concentrations. The stimulating effect of spermine and spermidine involves a facilitated binding of template RNA and aminoacylated tRNA to the ribosomes. In contrast, the aminoacylation of tRNA is not influenced. These data indicate that spermine as well as spermidine have regulatory functions during the translational process in cerebral cortex, cerebellum and liver. They seem to interact with ribosomal functions and to cause a shift in the optimal Mg2+ concentration required for the synthesis of brain and liver proteins."}
{"id": "PMID:487083", "title": "Cholinergic neuronotrophic factors: I. Survival, neurite outgrowth and choline acetyltransferase activity in monolayer cultures from chick embryo ciliary ganglia.", "content": "Two key components of neural development and regeneration, survival of the involved neurons and elongation of neuritic elements, are likely to depend on the availability of an appropriate trophic drive to these neurons. At present, only one trophic factor, Nerve Growth Factor, is known to ensure both survival and neuritic growth for its target neurons. A search for a second such agent, a putative cholinergic neuronotrophic factor (CNTF), has been undertaken using as indicators neuronal survival, neurite outgrowth and choline acetyltransferase (CAT) activity in monolayer cell cultures. Eight-day chick embryo ciliary ganglia yielded two monolayer culture systems which appear to be well suited for a CNTF assay. Ciliary ganglionic dissociates, seeded on a highly adhesive collagen substratum, show no neuronal survival by 24 h if the medium is supplemented only with serum or chick embryo extract. However serum and embryo extract combined support survival of, and extensive neuritic outgrowth from, nearly the theoretical number of ganglionic neurons seeded. Alternatively, ciliary ganglionic neurons can be made to survive and produce a profuse neuritic outgrowth on polyornithine-coated dishes if supplied with medium conditioned over chick embryo heart muscle cultures, as already described by other laboratories. The two trophic sources differ markedly in their effects on the ganglionic neurons when tested on collagen or polyornithine substrata, and in some cases when different serum supplements are used. Neuronal survival, neurite production and, possibly, CAT activity appear to be subject to independent regulation. The culture systems used in this study can be developed into quantitative bioassays for the isolation of the different agents responsible for neuronal survival and neurite promotion, and for the investigation of their activities.", "contents": "Cholinergic neuronotrophic factors: I. Survival, neurite outgrowth and choline acetyltransferase activity in monolayer cultures from chick embryo ciliary ganglia. Two key components of neural development and regeneration, survival of the involved neurons and elongation of neuritic elements, are likely to depend on the availability of an appropriate trophic drive to these neurons. At present, only one trophic factor, Nerve Growth Factor, is known to ensure both survival and neuritic growth for its target neurons. A search for a second such agent, a putative cholinergic neuronotrophic factor (CNTF), has been undertaken using as indicators neuronal survival, neurite outgrowth and choline acetyltransferase (CAT) activity in monolayer cell cultures. Eight-day chick embryo ciliary ganglia yielded two monolayer culture systems which appear to be well suited for a CNTF assay. Ciliary ganglionic dissociates, seeded on a highly adhesive collagen substratum, show no neuronal survival by 24 h if the medium is supplemented only with serum or chick embryo extract. However serum and embryo extract combined support survival of, and extensive neuritic outgrowth from, nearly the theoretical number of ganglionic neurons seeded. Alternatively, ciliary ganglionic neurons can be made to survive and produce a profuse neuritic outgrowth on polyornithine-coated dishes if supplied with medium conditioned over chick embryo heart muscle cultures, as already described by other laboratories. The two trophic sources differ markedly in their effects on the ganglionic neurons when tested on collagen or polyornithine substrata, and in some cases when different serum supplements are used. Neuronal survival, neurite production and, possibly, CAT activity appear to be subject to independent regulation. The culture systems used in this study can be developed into quantitative bioassays for the isolation of the different agents responsible for neuronal survival and neurite promotion, and for the investigation of their activities."}
{"id": "PMID:487084", "title": "Evidence for a cholinergic influence on catecholaminergic pathways terminating in the anterior and medial basal hypothalamus.", "content": "Adult male Sprague-Dawley rats were given hourly injections of physostigmine for 1--4 h, and the effect of this treatment on dopamine (DA) and noradrenaline (NA) content or on DA and NA was estimated by measuring the decline in these amines produced following the inhibition of tyrosine hydroxylase with alpha-methyl-p-tyrosine (alpha MPT). In later experiments oxotremorine was administered instead of physostigmine at hourly intervals for 2 h. Physostigmine administration resulted in a highly significant increase in the depletion of NA produced by alpha MPT indicating that the turnover of NA was increased by this drug. This effect was observed in the medial basal hypothalamus and anterior hypothalamus but not in the telencephalon-thalamus. Oxotremorine also produced an increase in NA turnover, but this drug was effective in all three brain areas. Atropine pretreatment blocked the effect of both physostigmine and oxotremorine on NA turnover. However, in the case of physostigmine, atropine was only effective if it was given 30 min before each injection of physostigmine. Mecamylamine, a nicotine blocker, did not reverse the effect of physostigmine on NA turnover. These results suggest that there is a cholinergic input via muscarinic receptors which influences the activity of noradrenergic pathways terminating in the anterior or medial basal hypothalamus.", "contents": "Evidence for a cholinergic influence on catecholaminergic pathways terminating in the anterior and medial basal hypothalamus. Adult male Sprague-Dawley rats were given hourly injections of physostigmine for 1--4 h, and the effect of this treatment on dopamine (DA) and noradrenaline (NA) content or on DA and NA was estimated by measuring the decline in these amines produced following the inhibition of tyrosine hydroxylase with alpha-methyl-p-tyrosine (alpha MPT). In later experiments oxotremorine was administered instead of physostigmine at hourly intervals for 2 h. Physostigmine administration resulted in a highly significant increase in the depletion of NA produced by alpha MPT indicating that the turnover of NA was increased by this drug. This effect was observed in the medial basal hypothalamus and anterior hypothalamus but not in the telencephalon-thalamus. Oxotremorine also produced an increase in NA turnover, but this drug was effective in all three brain areas. Atropine pretreatment blocked the effect of both physostigmine and oxotremorine on NA turnover. However, in the case of physostigmine, atropine was only effective if it was given 30 min before each injection of physostigmine. Mecamylamine, a nicotine blocker, did not reverse the effect of physostigmine on NA turnover. These results suggest that there is a cholinergic input via muscarinic receptors which influences the activity of noradrenergic pathways terminating in the anterior or medial basal hypothalamus."}
{"id": "PMID:487086", "title": "Immune lesions of central noradrenergic neurons produced by antibodies to dopamine-beta-hydroxylase.", "content": "(1) Intraventricular injection of antibodies to dopamine-beta-hydroxylase (DBH) caused degeneration of central noradrenergic nerve terminals in rats and guinea-pigs. In rats it was necessary to infuse exogenous complement in the form of guinea-pig serum together with the anti-DBH, whereas in guinea-pigs the anti-DBH was effective on its own. Control animals were infused with equivalent amounts of non-immune serum and complement and showed no signs of degeneration other than in the region of the needle tract. (2) There was a loss of varicosities in most terminal fields of the noradrenergic projections and swollen distorted axons were seen in both ascending and descending noradrenergic pathways. Noradrenergic cell bodies in the locus coeruleus and subcoeruleus appeared unaffected. No histochemical changes were observed in dopaminergic neurons. (3) The ultrastructural changes in degenerating axons that were first identifided by fluorescence histochemistry included swelling, vacuolation, accumulation of dense cored vesicles, lysosome-like bodies and smooth membranous sacs. The surrounding neuropil appeared normal. (4) There was a significant depletion of noradrenaline in all regions of the rat brain ranging from 20% in the hypothalamus to 80% in the neocortex. Dopamine concentrations were unaffected. (5) These observations provide a new approach to the production of selective lesions in specific neurotransmitter pathways that could be extended to non-adrenergic neurones. They may also be useful as a model for the study of autoimmune diseases of the nervous system.", "contents": "Immune lesions of central noradrenergic neurons produced by antibodies to dopamine-beta-hydroxylase. (1) Intraventricular injection of antibodies to dopamine-beta-hydroxylase (DBH) caused degeneration of central noradrenergic nerve terminals in rats and guinea-pigs. In rats it was necessary to infuse exogenous complement in the form of guinea-pig serum together with the anti-DBH, whereas in guinea-pigs the anti-DBH was effective on its own. Control animals were infused with equivalent amounts of non-immune serum and complement and showed no signs of degeneration other than in the region of the needle tract. (2) There was a loss of varicosities in most terminal fields of the noradrenergic projections and swollen distorted axons were seen in both ascending and descending noradrenergic pathways. Noradrenergic cell bodies in the locus coeruleus and subcoeruleus appeared unaffected. No histochemical changes were observed in dopaminergic neurons. (3) The ultrastructural changes in degenerating axons that were first identifided by fluorescence histochemistry included swelling, vacuolation, accumulation of dense cored vesicles, lysosome-like bodies and smooth membranous sacs. The surrounding neuropil appeared normal. (4) There was a significant depletion of noradrenaline in all regions of the rat brain ranging from 20% in the hypothalamus to 80% in the neocortex. Dopamine concentrations were unaffected. (5) These observations provide a new approach to the production of selective lesions in specific neurotransmitter pathways that could be extended to non-adrenergic neurones. They may also be useful as a model for the study of autoimmune diseases of the nervous system."}
{"id": "PMID:487087", "title": "The subcellular distribution of carnosine, carnosine synthetase, and carnosinase in mouse olfactory tissues.", "content": "The dipeptide, carnosine, its synthetic enzyme, carnosine synthetase, and its degradative enzyme, carnosinase, appear to be localized in the cytosol of mouse olfactory bulb and epithelium. Mouse olfactory bulbs and epithelium were prelabeled in vivo with [3H]carnosine following intranasal irrigation with [3H]beta-alanine. [3H]carnosine co-distributed in olfactory bulb with lactate dehydrogenase with only 10% in the crude mitochondrial fraction. Similar results were also seen with endogenous carnosine distribution. Over 70% of the carnosine present in the crude mitochondrial fraction was localized in synaptosomes following sucrose gradient centrifugation. However, further fractionation of vesicle containing fractions from osmotically lysed crude mitochondrial fractions indicated that [3H]carnosine was not associated with vesicles. Nearly 70% of all the [3H]carnosine present in olfactory epithelium was soluble with most of the remainder in the crude nuclear fraction. The enzymes carnosine synthetase and carnosinase were clearly soluble in olfactory epithelium with 98% and 85% of the activity in the cytosol. Less than 2% was found in the crude mitochondrial fraction. In olfactory bulb both enzymes also appeared soluble.", "contents": "The subcellular distribution of carnosine, carnosine synthetase, and carnosinase in mouse olfactory tissues. The dipeptide, carnosine, its synthetic enzyme, carnosine synthetase, and its degradative enzyme, carnosinase, appear to be localized in the cytosol of mouse olfactory bulb and epithelium. Mouse olfactory bulbs and epithelium were prelabeled in vivo with [3H]carnosine following intranasal irrigation with [3H]beta-alanine. [3H]carnosine co-distributed in olfactory bulb with lactate dehydrogenase with only 10% in the crude mitochondrial fraction. Similar results were also seen with endogenous carnosine distribution. Over 70% of the carnosine present in the crude mitochondrial fraction was localized in synaptosomes following sucrose gradient centrifugation. However, further fractionation of vesicle containing fractions from osmotically lysed crude mitochondrial fractions indicated that [3H]carnosine was not associated with vesicles. Nearly 70% of all the [3H]carnosine present in olfactory epithelium was soluble with most of the remainder in the crude nuclear fraction. The enzymes carnosine synthetase and carnosinase were clearly soluble in olfactory epithelium with 98% and 85% of the activity in the cytosol. Less than 2% was found in the crude mitochondrial fraction. In olfactory bulb both enzymes also appeared soluble."}
{"id": "PMID:487088", "title": "Deficits in luminous flux discimination by cats with lesions of the superior colliculus-pretectum.", "content": "(1) Intact cats and cats relearning or initially learning after bilateral ablation of the superior colliculus-pretectum were trained on a simple flux discrimination in a straight maze where the stimuli were either immediately side by side at the end of the runway or were separated by an opaque barrier. (2) After ablation of the superior colliculus-pretectum, each preoperatively trained cat had a significant deficit in retention of the luminous flux discrimination regardless of whether or not the stimuli were separated by a barrier or whether the correct stimulus was darker or lighter than the incorrect stimulus. (3) Under each of the different training conditions, every cat learning the flux discrimination initially after ablation of the superior colliculus-pretectum was retarded in comparison with intact cats. (4) The severity of the postoperative learning deficit correlated highly with the total extent of the lesion in the superior colliculus and pretectum.", "contents": "Deficits in luminous flux discimination by cats with lesions of the superior colliculus-pretectum. (1) Intact cats and cats relearning or initially learning after bilateral ablation of the superior colliculus-pretectum were trained on a simple flux discrimination in a straight maze where the stimuli were either immediately side by side at the end of the runway or were separated by an opaque barrier. (2) After ablation of the superior colliculus-pretectum, each preoperatively trained cat had a significant deficit in retention of the luminous flux discrimination regardless of whether or not the stimuli were separated by a barrier or whether the correct stimulus was darker or lighter than the incorrect stimulus. (3) Under each of the different training conditions, every cat learning the flux discrimination initially after ablation of the superior colliculus-pretectum was retarded in comparison with intact cats. (4) The severity of the postoperative learning deficit correlated highly with the total extent of the lesion in the superior colliculus and pretectum."}
{"id": "PMID:487089", "title": "The effects of L-dopa on dorsal horn cell responses to innocuous skin stimulation.", "content": "The effects of L-DOPA on responses of dorsal horn cells to innocuous mechanical skin stimulation were studied. Following intravenous administration of L-DOPA (10--40 mg/kg) to cats with intact spinal cords, dorsal horn cells, which could be activated by only innocuous or innocuous and noxious stimuli, demonstrated increased reponses manifested by an increase in the average number of spikes per stimulus, increased receptive field size and occasional changes in adequate stimuli. When cats with acute cord transections were studied, L-DOPA increased the responses of cells located in lamina 4 and those cells which responded only to innocuous stimuli; cells which responded to noxious stimuli and those located in other laminae had depressed responses following L-DOPA administration. The inhibitory effects of L-DOPA were in part abolished, in spinal cats, by pretreatment with parachlorophenylalanine, a serotonin depletor. Studies of dorsal cells in chronic spinal cats indicated that the effects of L-DOPA were largely dependent on functioning terminals of descending suprasegmental pathways. The conclusion was reached that descending noradrenergic pathways, indirectly, cause an increase in transmission from cutaneous afferents to dorsal horn cells and that some of the inhibitory effects of L-DOPA described by others are a manifestation of L-DOPA interactions with inhibitory serotonergic systems.", "contents": "The effects of L-dopa on dorsal horn cell responses to innocuous skin stimulation. The effects of L-DOPA on responses of dorsal horn cells to innocuous mechanical skin stimulation were studied. Following intravenous administration of L-DOPA (10--40 mg/kg) to cats with intact spinal cords, dorsal horn cells, which could be activated by only innocuous or innocuous and noxious stimuli, demonstrated increased reponses manifested by an increase in the average number of spikes per stimulus, increased receptive field size and occasional changes in adequate stimuli. When cats with acute cord transections were studied, L-DOPA increased the responses of cells located in lamina 4 and those cells which responded only to innocuous stimuli; cells which responded to noxious stimuli and those located in other laminae had depressed responses following L-DOPA administration. The inhibitory effects of L-DOPA were in part abolished, in spinal cats, by pretreatment with parachlorophenylalanine, a serotonin depletor. Studies of dorsal cells in chronic spinal cats indicated that the effects of L-DOPA were largely dependent on functioning terminals of descending suprasegmental pathways. The conclusion was reached that descending noradrenergic pathways, indirectly, cause an increase in transmission from cutaneous afferents to dorsal horn cells and that some of the inhibitory effects of L-DOPA described by others are a manifestation of L-DOPA interactions with inhibitory serotonergic systems."}
{"id": "PMID:487090", "title": "Conductance changes during bath application of beta-alanine and taurine in giant interneurons of the isolated lamprey spinal cord.", "content": "(1) Input conductances of giant interneurons in the isolated spinal cord of lampreys were measured with two separate intracellular electrodes. Bath applications of 0.4--3 mM beta-alanine and taurine produced large, reversible conductance increases which were Cl-dependent. (2) Strychnine at 1--2.5 microM might be a competitive antagonist of both amino acids, but had a stronger effect on taurine. Bicuculline and picrotoxin were weak antagonists of beta-alanine and taurine in some cells. (3) A few giant interneurons desensitized after repeated application of the amino acids, but most became more sensitive. Responses to the amino acids also increase at low temperatures in in Na-free fluid, suggesting effect of uptake mechanisms.", "contents": "Conductance changes during bath application of beta-alanine and taurine in giant interneurons of the isolated lamprey spinal cord. (1) Input conductances of giant interneurons in the isolated spinal cord of lampreys were measured with two separate intracellular electrodes. Bath applications of 0.4--3 mM beta-alanine and taurine produced large, reversible conductance increases which were Cl-dependent. (2) Strychnine at 1--2.5 microM might be a competitive antagonist of both amino acids, but had a stronger effect on taurine. Bicuculline and picrotoxin were weak antagonists of beta-alanine and taurine in some cells. (3) A few giant interneurons desensitized after repeated application of the amino acids, but most became more sensitive. Responses to the amino acids also increase at low temperatures in in Na-free fluid, suggesting effect of uptake mechanisms."}
{"id": "PMID:487092", "title": "Tibialis anterior response to sudden ankle displacements in normal and Parkinsonian subjects.", "content": "It is well known that in Parkinsonian subjects with akinesia, reaction times are increased but reflex latencies remain normal. We have attempted to use this knowledge to distinguish between 'reflex' and 'voluntary' components of the electromyographic (EMG) response to ankle displacement. The EMG and torque responses of tibialis anterior (TA) to randomly applied servo-controlled plantar-flexing displacements of the ankle with and without the subject's intentional opposition were examined in 9 Parkinsonian and 9 age-matched normal humans. To obtain a measure of akinesia, the response latency to a visual stimulus was subsequently measured in the same subjects. Three principal findings emerged. (1) The intermediate latency EMG component (PSR) of the response evoked by ankle displacement with the subject instructed to relax was more regularly evoked and of lower threshold in Parkinsonians than in normals. This finding corresponds to the enlarged M2 component in upper limb muscles. However, the facilitation of PSR was not found to be associated with an increase in torque. In fact, the patients did not exhibit more stiffness than normals under our experimental conditions. (2) Mean latency estimate of the PSR was indistinguishable between Parkinsonians and normals. This finding puts the PSR in the nature of a reflex. Indeed, in accordance with reflex behaviour which is proportional to input characteristics, its area increased linearly with increase in the magnitude of displacement velocity. (3) In contrast, the 'late' EMG response (FSR) evoked by opposing sudden ankle displacement exhibited a significantly longer latency in 6 out of 8 Parkinsonians than normals. In the same patients, the EMG response latency to a visual signal was similarly increased. The delay of FSR in akinesia patients thus argued against its being a stereotyped reflex. The result is discussed with reference to the recent finding that preprogrammed responses are delayed in Parkinsonians.", "contents": "Tibialis anterior response to sudden ankle displacements in normal and Parkinsonian subjects. It is well known that in Parkinsonian subjects with akinesia, reaction times are increased but reflex latencies remain normal. We have attempted to use this knowledge to distinguish between 'reflex' and 'voluntary' components of the electromyographic (EMG) response to ankle displacement. The EMG and torque responses of tibialis anterior (TA) to randomly applied servo-controlled plantar-flexing displacements of the ankle with and without the subject's intentional opposition were examined in 9 Parkinsonian and 9 age-matched normal humans. To obtain a measure of akinesia, the response latency to a visual stimulus was subsequently measured in the same subjects. Three principal findings emerged. (1) The intermediate latency EMG component (PSR) of the response evoked by ankle displacement with the subject instructed to relax was more regularly evoked and of lower threshold in Parkinsonians than in normals. This finding corresponds to the enlarged M2 component in upper limb muscles. However, the facilitation of PSR was not found to be associated with an increase in torque. In fact, the patients did not exhibit more stiffness than normals under our experimental conditions. (2) Mean latency estimate of the PSR was indistinguishable between Parkinsonians and normals. This finding puts the PSR in the nature of a reflex. Indeed, in accordance with reflex behaviour which is proportional to input characteristics, its area increased linearly with increase in the magnitude of displacement velocity. (3) In contrast, the 'late' EMG response (FSR) evoked by opposing sudden ankle displacement exhibited a significantly longer latency in 6 out of 8 Parkinsonians than normals. In the same patients, the EMG response latency to a visual signal was similarly increased. The delay of FSR in akinesia patients thus argued against its being a stereotyped reflex. The result is discussed with reference to the recent finding that preprogrammed responses are delayed in Parkinsonians."}
{"id": "PMID:487101", "title": "Spinal projections from the lower brain stem in the cat as demonstrated by the horseradish peroxidase technique. I. Origins of the reticulospinal tracts and their funicular trajectories.", "content": "Using a retrograde tracer technique with horseradish peroxidase (HRP) attempts were made to determine the origins of reticulospinal tracts and their funicular trajectories. Reticulospinal tracts originating from the mesencephalic reticular formation (RF) were composed of: (1) descending projections arising from the cluster of cells located just lateral to the periaqueductal gray that course in the anterior funiculus (AF) and ventral part of the lateral funiculus (LF) with ipsilateral predominance; and (2) projections from the cluster of cells located dorsal to the brachium conjunctivum that course in the ipsilateral LF. Origins of the pontine reticulospinal tracts arising from the n. reticularis pontis oralis (Poo) have been divided qnto three parts: (1) medial one-third; (2) middle; and (3) ventrolateral. The axons from the medial part descend ipsilaterally via the medial part of the AF, while the axons from the ventrolateral part of the Poo give rise to diffuse descending projections in the AF and LF. The middle part of the Poo has been further subdivided into: (1) dorsal part that gives rise to spinal projections ipsilaterally in the ventrolateral funiculus (VLF); and (2) ventral, particularly its upper part, whose axons descend bilaterally via the DLF. Origins of reticulospinal tracts from the n. reticularis pontis caudalis (Poc) could be divided into three parts: (1) medial; (2) dorsolateral; and (3) ventrolateral. The medial part of the Poc is a source of axons via the medial part of the ipsilateral AF, while the ventrolateral part of the nucleus is a source of axons via the contralateral LF. The spinal projections from the dorsolateral part of the Poc appears to course diffusely in the AF and LF, but with DLF predominance. The n. reticularis gigantocellularis (Gc) was found to be a main medullary source of the spinal projections in the ipsilateral AF, while n. reticularis magnocellularis (Mc) is the major source of the fibers coursing ipsilaterally in the VLF. The most medial part of the Mc descends ipsilaterally via the medial part of the AF, while the ventrolateral part of the nucleus together with the n. reticularis lateralis of Meesen and Olszewski descends ipsilaterally via the DLF. It has also been found that the axons from the n. reticularis paramedianus pass via both the AF and LF with ipsilateral predominance, while the n. reticularis dorsalis and ventralis course via the LF with ipsilateral predominance.", "contents": "Spinal projections from the lower brain stem in the cat as demonstrated by the horseradish peroxidase technique. I. Origins of the reticulospinal tracts and their funicular trajectories. Using a retrograde tracer technique with horseradish peroxidase (HRP) attempts were made to determine the origins of reticulospinal tracts and their funicular trajectories. Reticulospinal tracts originating from the mesencephalic reticular formation (RF) were composed of: (1) descending projections arising from the cluster of cells located just lateral to the periaqueductal gray that course in the anterior funiculus (AF) and ventral part of the lateral funiculus (LF) with ipsilateral predominance; and (2) projections from the cluster of cells located dorsal to the brachium conjunctivum that course in the ipsilateral LF. Origins of the pontine reticulospinal tracts arising from the n. reticularis pontis oralis (Poo) have been divided qnto three parts: (1) medial one-third; (2) middle; and (3) ventrolateral. The axons from the medial part descend ipsilaterally via the medial part of the AF, while the axons from the ventrolateral part of the Poo give rise to diffuse descending projections in the AF and LF. The middle part of the Poo has been further subdivided into: (1) dorsal part that gives rise to spinal projections ipsilaterally in the ventrolateral funiculus (VLF); and (2) ventral, particularly its upper part, whose axons descend bilaterally via the DLF. Origins of reticulospinal tracts from the n. reticularis pontis caudalis (Poc) could be divided into three parts: (1) medial; (2) dorsolateral; and (3) ventrolateral. The medial part of the Poc is a source of axons via the medial part of the ipsilateral AF, while the ventrolateral part of the nucleus is a source of axons via the contralateral LF. The spinal projections from the dorsolateral part of the Poc appears to course diffusely in the AF and LF, but with DLF predominance. The n. reticularis gigantocellularis (Gc) was found to be a main medullary source of the spinal projections in the ipsilateral AF, while n. reticularis magnocellularis (Mc) is the major source of the fibers coursing ipsilaterally in the VLF. The most medial part of the Mc descends ipsilaterally via the medial part of the AF, while the ventrolateral part of the nucleus together with the n. reticularis lateralis of Meesen and Olszewski descends ipsilaterally via the DLF. It has also been found that the axons from the n. reticularis paramedianus pass via both the AF and LF with ipsilateral predominance, while the n. reticularis dorsalis and ventralis course via the LF with ipsilateral predominance."}
{"id": "PMID:487102", "title": "The regression of synapses formed by a foreign nerve in a mature axolotl striated muscle.", "content": "A study has been made of the factors which determine that the terminals of a foreign flexor nerve at synaptic sites in a unrodele extensor muscle regress on return of the original extensor nerve. The quantal content (m) of the endplate potential (EPP) at flexor nerve terminal synapses, during innervation of a previously denervated extensor muscle, increased in about 8 weeks to reach the same size as at normal extensor nerve terminals; the same time was taken for m to reach normal size at extensor nerve terminals when these reinnervated their own muscle. At flexor nerve terminals, m decreased eventually to zero if the extensor nerve terminals returned within about 6 weeks of synapse formation by the flexor nerve terminals to the same or an immediately adjacent synaptic site to that occupied by these terminals. During this decrease in m at flexor nerve terminals, stimulation of the flexor nerves in the presence of horseradish peroxidase (HRP) showed HRP-labelled flexor nerve terminals present only in those regions of the extensor muscle in which the electrical signs of flexor nerve terminals were observed, indicating that the decrease in m at regressing flexor terminals was accompanied by their vacating synaptic sites. However, flexor nerve terminals failed to regress from the extensor muscle on return of the original nerve supply if the flexor nerve allowed to form synapses for more than about 10 weeks before the return of the extensor nerve.", "contents": "The regression of synapses formed by a foreign nerve in a mature axolotl striated muscle. A study has been made of the factors which determine that the terminals of a foreign flexor nerve at synaptic sites in a unrodele extensor muscle regress on return of the original extensor nerve. The quantal content (m) of the endplate potential (EPP) at flexor nerve terminal synapses, during innervation of a previously denervated extensor muscle, increased in about 8 weeks to reach the same size as at normal extensor nerve terminals; the same time was taken for m to reach normal size at extensor nerve terminals when these reinnervated their own muscle. At flexor nerve terminals, m decreased eventually to zero if the extensor nerve terminals returned within about 6 weeks of synapse formation by the flexor nerve terminals to the same or an immediately adjacent synaptic site to that occupied by these terminals. During this decrease in m at flexor nerve terminals, stimulation of the flexor nerves in the presence of horseradish peroxidase (HRP) showed HRP-labelled flexor nerve terminals present only in those regions of the extensor muscle in which the electrical signs of flexor nerve terminals were observed, indicating that the decrease in m at regressing flexor terminals was accompanied by their vacating synaptic sites. However, flexor nerve terminals failed to regress from the extensor muscle on return of the original nerve supply if the flexor nerve allowed to form synapses for more than about 10 weeks before the return of the extensor nerve."}
{"id": "PMID:487103", "title": "Intracellular analysis of the development of responses of caudate neurons to stimulation of cortex, thalamus and substantia nigra in the kitten.", "content": "Intracellular recordings were made from caudate neurons in anesthetized kittens of 2-72 days of age. In adult cats, results of intracellular recordings indicate that caudate neurons respond most frequently to stimulation of their major afferents from cortex, thalamus and substantia nigra with a sequence of excitation followed by inhibition (EPSP-IPSP sequence). The results of the present study show that the prominent IPSP of this sequence is not well developed in young kittens and does not reach adult values in terms of frequency of occurrence until beyond 40 days of age. Amplitude and duration of the IPSP evoked by cortical stimulation also did not reach adult values until beyond 40 days of age. In contrast, EPSPs can be evoked in the youngest kittens by stimulation of afferents to the caudate. These findings suggest that the caudate nucleus may alter its role during development. In early postnatal periods it functions as a simple relay system transmitting incoming information to its outputs in a relatively unaltered fashion. Later in development it becomes a system capable of complex modulation and filtering of neural information.", "contents": "Intracellular analysis of the development of responses of caudate neurons to stimulation of cortex, thalamus and substantia nigra in the kitten. Intracellular recordings were made from caudate neurons in anesthetized kittens of 2-72 days of age. In adult cats, results of intracellular recordings indicate that caudate neurons respond most frequently to stimulation of their major afferents from cortex, thalamus and substantia nigra with a sequence of excitation followed by inhibition (EPSP-IPSP sequence). The results of the present study show that the prominent IPSP of this sequence is not well developed in young kittens and does not reach adult values in terms of frequency of occurrence until beyond 40 days of age. Amplitude and duration of the IPSP evoked by cortical stimulation also did not reach adult values until beyond 40 days of age. In contrast, EPSPs can be evoked in the youngest kittens by stimulation of afferents to the caudate. These findings suggest that the caudate nucleus may alter its role during development. In early postnatal periods it functions as a simple relay system transmitting incoming information to its outputs in a relatively unaltered fashion. Later in development it becomes a system capable of complex modulation and filtering of neural information."}
{"id": "PMID:487113", "title": "The organization of the efferent projections of the substantia nigra in the rat. A retrograde fluorescent double labeling study.", "content": "The organization of three of the efferent projections of substantia nigra has been investigated in rat by means of combined injections of two fluorescent retrograde tracers: red fluorescent Evans Blue and blue fluorescent DAPI-primuline mixture. First the distributions of the retrogradely labeled neurons in substantia nigra after injections of each of the two tracers in the striatum, thalamus and superior colliculus were compared with the distributions of the retrogradely labeled nigral neurons after HRP injections in these same structures. The findings in these sets of experiments were the same. This indicates that the two fluorescent retrograde tracers are as effective as HRP in tracing the efferent fiber connections of the substantia nigra. Subsequently the retrograde labeling of the substantia nigra neurons was studied after combined injections of the two fluorescent retrograde tracers in two of the above structures, in different combinations. In these experiments both single labeled neurons fluorescing either red or blue, as well as double labeled neurons fluorescing both red and blue were found. Double labeled neurons were present only in pars reticulata and occurred only after injections of the two tracers in thalamus and superior colliculus respectively. From this it has been concluded that in rat the various efferent projections from the substantia nigra are mainly derived from different neurons, but that at least part of the neurons which distribute fibers to superior colliculus also distributes collaterals to the thalamus.", "contents": "The organization of the efferent projections of the substantia nigra in the rat. A retrograde fluorescent double labeling study. The organization of three of the efferent projections of substantia nigra has been investigated in rat by means of combined injections of two fluorescent retrograde tracers: red fluorescent Evans Blue and blue fluorescent DAPI-primuline mixture. First the distributions of the retrogradely labeled neurons in substantia nigra after injections of each of the two tracers in the striatum, thalamus and superior colliculus were compared with the distributions of the retrogradely labeled nigral neurons after HRP injections in these same structures. The findings in these sets of experiments were the same. This indicates that the two fluorescent retrograde tracers are as effective as HRP in tracing the efferent fiber connections of the substantia nigra. Subsequently the retrograde labeling of the substantia nigra neurons was studied after combined injections of the two fluorescent retrograde tracers in two of the above structures, in different combinations. In these experiments both single labeled neurons fluorescing either red or blue, as well as double labeled neurons fluorescing both red and blue were found. Double labeled neurons were present only in pars reticulata and occurred only after injections of the two tracers in thalamus and superior colliculus respectively. From this it has been concluded that in rat the various efferent projections from the substantia nigra are mainly derived from different neurons, but that at least part of the neurons which distribute fibers to superior colliculus also distributes collaterals to the thalamus."}
{"id": "PMID:487114", "title": "Permeability of the blood-brain barrier to mannitol in the rat following 2450 MHz microwave irradiation.", "content": "The radiotracer method of Oldendorf was used to determine if 2450 MHz continuous wave (CW) microwave energy increases blood-brain barrier permeability to [14C]mannitol, which is normally excluded from entering the brain. Anesthetized, adult rats were irradiated singly for 30 min in the quiet zone of an anechoic chamber, at average power densities from 0.1 to 30 mW/sq.cm. Afterwards each rat received an intracarotid bolus injection of [14C]mannitol/[3H]water mixture and was decapitated 15 sec later. Uptake of [14C]mannitol relative to the highly permeable reference substance, [3H]water, was calculated as the brain uptake index (BUI) for 4 brain regions. Mean BUI values for tissues from the microwave-irradiated rats did not differ significantly from sham-irradiated animals, and a microwave influence on barrier permeability was not evident. Irrespective of treatment, BUI values for cerebellum and medulla were much higher and more variable than values for cortex or diencephalon, and were associated with reduced absorbance or retention of [3H]water. Because of a compromising influence of the vertebral arterial supply on the distribution of intracarotid-injected radiotracers, BUI measurements in caudal brain regions are probably unreliable unless accompanied by data on regional radioisotope concentrations. The absence of such data in an earlier BUI study, suggests that increases in BUI for cerebellum and medulla attributed to microwaves were possibly misinterpreted as differences in barrier permeability to [14C]saccharides, when in fact changes in blood flow and [3H]water influx or egress were responsible.", "contents": "Permeability of the blood-brain barrier to mannitol in the rat following 2450 MHz microwave irradiation. The radiotracer method of Oldendorf was used to determine if 2450 MHz continuous wave (CW) microwave energy increases blood-brain barrier permeability to [14C]mannitol, which is normally excluded from entering the brain. Anesthetized, adult rats were irradiated singly for 30 min in the quiet zone of an anechoic chamber, at average power densities from 0.1 to 30 mW/sq.cm. Afterwards each rat received an intracarotid bolus injection of [14C]mannitol/[3H]water mixture and was decapitated 15 sec later. Uptake of [14C]mannitol relative to the highly permeable reference substance, [3H]water, was calculated as the brain uptake index (BUI) for 4 brain regions. Mean BUI values for tissues from the microwave-irradiated rats did not differ significantly from sham-irradiated animals, and a microwave influence on barrier permeability was not evident. Irrespective of treatment, BUI values for cerebellum and medulla were much higher and more variable than values for cortex or diencephalon, and were associated with reduced absorbance or retention of [3H]water. Because of a compromising influence of the vertebral arterial supply on the distribution of intracarotid-injected radiotracers, BUI measurements in caudal brain regions are probably unreliable unless accompanied by data on regional radioisotope concentrations. The absence of such data in an earlier BUI study, suggests that increases in BUI for cerebellum and medulla attributed to microwaves were possibly misinterpreted as differences in barrier permeability to [14C]saccharides, when in fact changes in blood flow and [3H]water influx or egress were responsible."}
{"id": "PMID:487115", "title": "Compartments of tubulin and tubulin-like proteins in differentiating neubroblastoma cells.", "content": "Cytoplasmic, tubular and particulate fractions of differentiating neuroblastoma cells were prepared and the tubulin together with tubulin-like proteins was measured in each cell fraction during different stages of cell differentiation. In undifferentiated cells, 73%, 5% and 22% of the tubulin and tubulin-like proteins were contained in the cytoplasmic, tubular and particulate fractions, respectively. After 5 days of differentiation, the overall content of tubulin and tubulin-like proteins had increased by 73%. This corresponded to increases of 45%, 145% and 100% in the cytoplasmic, microtubular and particulate fractions, respectively. The increase in membrane-bound (particulate) tubulin and tubulin-like proteins was significantly greater than the total increase of proteins in the particulate fraction. Polyacrylamide gel electrophoresis of the proteins in each subcellular fraction revealed the presence of protein bands corresponding to the alpha and beta subunits of tubulin. Whereas these bands indicated equal amounts of protein in the alpha and beta positions for the tubular and particulate cell fractions, an analysis of the cytoplasmic fraction revealed much more protein migrating to the alpha-tubulin position than to the beta-tubulin position, especially during cell differentiation. Furthermore, two overlapping but distinct protein bands were demonstrable in the position of the alpha-tubulin from the cytoplasmic fraction. These bands were designated alpha 1 and alpha 2. The particulate fraction contained only the alpha 1 and the tubular fraction only the alpha 2 protein band. The addition of 1 mM dibutyryl cyclic AMP to the neuroblastoma cells, at the time when the serum was withdrawn, enhanced the rate of differentiation and the redistribution of tubulin and tubulin-like proteins within the 3 cellular compartments. These results are discussed as they relate to the regulation, biosynthesis, turnover and compartmentation of tubulin and tubulin-like proteins in differentiating neuroblastoma cells.", "contents": "Compartments of tubulin and tubulin-like proteins in differentiating neubroblastoma cells. Cytoplasmic, tubular and particulate fractions of differentiating neuroblastoma cells were prepared and the tubulin together with tubulin-like proteins was measured in each cell fraction during different stages of cell differentiation. In undifferentiated cells, 73%, 5% and 22% of the tubulin and tubulin-like proteins were contained in the cytoplasmic, tubular and particulate fractions, respectively. After 5 days of differentiation, the overall content of tubulin and tubulin-like proteins had increased by 73%. This corresponded to increases of 45%, 145% and 100% in the cytoplasmic, microtubular and particulate fractions, respectively. The increase in membrane-bound (particulate) tubulin and tubulin-like proteins was significantly greater than the total increase of proteins in the particulate fraction. Polyacrylamide gel electrophoresis of the proteins in each subcellular fraction revealed the presence of protein bands corresponding to the alpha and beta subunits of tubulin. Whereas these bands indicated equal amounts of protein in the alpha and beta positions for the tubular and particulate cell fractions, an analysis of the cytoplasmic fraction revealed much more protein migrating to the alpha-tubulin position than to the beta-tubulin position, especially during cell differentiation. Furthermore, two overlapping but distinct protein bands were demonstrable in the position of the alpha-tubulin from the cytoplasmic fraction. These bands were designated alpha 1 and alpha 2. The particulate fraction contained only the alpha 1 and the tubular fraction only the alpha 2 protein band. The addition of 1 mM dibutyryl cyclic AMP to the neuroblastoma cells, at the time when the serum was withdrawn, enhanced the rate of differentiation and the redistribution of tubulin and tubulin-like proteins within the 3 cellular compartments. These results are discussed as they relate to the regulation, biosynthesis, turnover and compartmentation of tubulin and tubulin-like proteins in differentiating neuroblastoma cells."}
{"id": "PMID:487121", "title": "Tactile neurons of the superior colliculus of the cat: input and physiological properties.", "content": "Neurons in the strata intermediale and profundum of the cat superior colliculus (SC) previously have been shown to be organized somatotopically and to respond to tactile stimuli. The stimulus properties required for neuronal activation suggest that these neurons are excited by inputs from selected classes of high-velocity, sensitive cutaneous mechanoreceptors, and that stimulus-response relationships indicate only a narrow range of information concerning stimulus magnitude. The properties of these neurons have been interpreted as serving a role in stimulus detection that recovers slowly and may be best suited for directed attention and orientation behavior. The input to the SC was studied by injection of horseradish peroxidase (HRP) for retrograde labeling of neurons projecting to this region. The main inputs subserving the tactile pathway are the principal trigeminal nucleus and the rostral portion of the spinal V subnucleus oralis and the main cuneate nucleus. Sparser labeling was found in other somatic relays, including the gracile and lateral cervical nuclei and posterior portion of the spinal trigeminal complex. Several other projections relating to visual mechanisms are also described.", "contents": "Tactile neurons of the superior colliculus of the cat: input and physiological properties. Neurons in the strata intermediale and profundum of the cat superior colliculus (SC) previously have been shown to be organized somatotopically and to respond to tactile stimuli. The stimulus properties required for neuronal activation suggest that these neurons are excited by inputs from selected classes of high-velocity, sensitive cutaneous mechanoreceptors, and that stimulus-response relationships indicate only a narrow range of information concerning stimulus magnitude. The properties of these neurons have been interpreted as serving a role in stimulus detection that recovers slowly and may be best suited for directed attention and orientation behavior. The input to the SC was studied by injection of horseradish peroxidase (HRP) for retrograde labeling of neurons projecting to this region. The main inputs subserving the tactile pathway are the principal trigeminal nucleus and the rostral portion of the spinal V subnucleus oralis and the main cuneate nucleus. Sparser labeling was found in other somatic relays, including the gracile and lateral cervical nuclei and posterior portion of the spinal trigeminal complex. Several other projections relating to visual mechanisms are also described."}
{"id": "PMID:487122", "title": "Influence of midbrain stimulation on the excitability of neurons in the medial hypothalamus of the rat.", "content": "Extracellular action potentials were recorded from 1098 neurons in the medial hypothalamus of pentobarbital anesthetized male rats. Their excitability was analyzed after single 1 Hz stimulation of the midbrain periaqueductal gray (PAG) or adjacent reticular formation. Cells were also examined for their response to median eminence (ME), amygdala, lateral septum (LS) or anterior hypothalamic/preoptic area (AHA/POA) stimulation. Antidromic invasion from midbrain stimulation was recorded from 110 neurons. Eight of these neurons showed features of axon branching and displayed antidromic invasion from both midbrain and amygdala (2 cells) or AHA/POA (6 cells). Many neurons with midbrain projections displayed orthodromic responses to stimulation in the amygdala, but few responded to AHA/POA or LS stimulation. Midbrain stimulation evoked orthodromic responses from 99 medial hypothalamic neurons. Many of these cells also displayed orthodromic responses to amygdala or AHA/POA stimulation, whereas a small number were activated antidromically by stimulation in these sites. None of 42 neurons activated antidromically from median eminence stimulation were responsive to midbrain stimulation. These results provide electrophysiological evidence of reciprocal connections between medial hypothalamic and medial midbrain areas, and indicate that medial hypothalamic neurons with midbrain connections are subject to influences from other extrahypothalamic areas.", "contents": "Influence of midbrain stimulation on the excitability of neurons in the medial hypothalamus of the rat. Extracellular action potentials were recorded from 1098 neurons in the medial hypothalamus of pentobarbital anesthetized male rats. Their excitability was analyzed after single 1 Hz stimulation of the midbrain periaqueductal gray (PAG) or adjacent reticular formation. Cells were also examined for their response to median eminence (ME), amygdala, lateral septum (LS) or anterior hypothalamic/preoptic area (AHA/POA) stimulation. Antidromic invasion from midbrain stimulation was recorded from 110 neurons. Eight of these neurons showed features of axon branching and displayed antidromic invasion from both midbrain and amygdala (2 cells) or AHA/POA (6 cells). Many neurons with midbrain projections displayed orthodromic responses to stimulation in the amygdala, but few responded to AHA/POA or LS stimulation. Midbrain stimulation evoked orthodromic responses from 99 medial hypothalamic neurons. Many of these cells also displayed orthodromic responses to amygdala or AHA/POA stimulation, whereas a small number were activated antidromically by stimulation in these sites. None of 42 neurons activated antidromically from median eminence stimulation were responsive to midbrain stimulation. These results provide electrophysiological evidence of reciprocal connections between medial hypothalamic and medial midbrain areas, and indicate that medial hypothalamic neurons with midbrain connections are subject to influences from other extrahypothalamic areas."}
{"id": "PMID:487123", "title": "Release of [3H]serotonin and its binding protein from enteric neurons.", "content": "The release of [3H]5-HT and its binding protein, SBP, from the guinea pig enteric nervous system was analyzed. Release of both [3H]5-HT and [3H]NE from strips of longitudinal muscle with adherent myenteric plexus preloaded with the respective radioactive amine was evoked by high K+ and the ionophore X537A. However, Ca2+-dependence could not be shown for [3H]5-HT release by either agent or for [3H]NE release by X537A. However, Ca2+-dependence (as well as inhibition of release by high Mg2+ and tetrodotoxin) could be demonstrated for the release of radioactivity evoked by electrical field stimulation of everted segments of ileum preloaded by perfusion through the serosal lumen with [3H]5-HT. Light and electron microscopic radioautography revealed that the sources of released radioactivity were axons, especially axonal varicosities containing a mixture of small clear and large dense-cored vesicles. SPB, but not the cytosol marker protein, lactic dehydrogenase, was spontaneously released from the perfused everted ileum. A marked increase in SBP (but not LDH) release was provoked by electrical field stimulation at 10 Hz, and this increased release (but not the spontaneous release) was Ca2+-dependent. It is concluded that SBP and 5-HT are probably stored together, at least in part in vesicles, and that both can be released by exocytosis from depolarized axon terminals.", "contents": "Release of [3H]serotonin and its binding protein from enteric neurons. The release of [3H]5-HT and its binding protein, SBP, from the guinea pig enteric nervous system was analyzed. Release of both [3H]5-HT and [3H]NE from strips of longitudinal muscle with adherent myenteric plexus preloaded with the respective radioactive amine was evoked by high K+ and the ionophore X537A. However, Ca2+-dependence could not be shown for [3H]5-HT release by either agent or for [3H]NE release by X537A. However, Ca2+-dependence (as well as inhibition of release by high Mg2+ and tetrodotoxin) could be demonstrated for the release of radioactivity evoked by electrical field stimulation of everted segments of ileum preloaded by perfusion through the serosal lumen with [3H]5-HT. Light and electron microscopic radioautography revealed that the sources of released radioactivity were axons, especially axonal varicosities containing a mixture of small clear and large dense-cored vesicles. SPB, but not the cytosol marker protein, lactic dehydrogenase, was spontaneously released from the perfused everted ileum. A marked increase in SBP (but not LDH) release was provoked by electrical field stimulation at 10 Hz, and this increased release (but not the spontaneous release) was Ca2+-dependent. It is concluded that SBP and 5-HT are probably stored together, at least in part in vesicles, and that both can be released by exocytosis from depolarized axon terminals."}
{"id": "PMID:487124", "title": "Effect of midbrain raphe lesion or 5,7-dihydroxytryptamine treatment on the prolactin-releasing action of quipazine and D-fenfluramine in rats.", "content": "The role of brain serotonin in regulating prolactin (PRL) secretion has been investigated by studying the effect of quipazine and D-fenfluramine, two serotonin-like drugs, on plasma PRL levels under various experimental conditions. Quipazine (5, 10 and 20 mg/kg i.p.) and D-fenfluramine (5, 7.5 and 10 mg/kg i.p.) induced dose-related increases in plasma PRL levels in male rats. Intraventricular injection of 5,7-dihydroxytryptamine (5,7-DHT) or electrolytic lesion of the nucleus raphe medianus (MR), which caused a marked and selective depletion of hypothalamic serotonin levels, significantly reduced the PRL-releasing effect of both quipazine and D-fenfluramine. These results suggest that the effect of these drugs on PRL release is mediated through a serotonergic mechanism in the brain.", "contents": "Effect of midbrain raphe lesion or 5,7-dihydroxytryptamine treatment on the prolactin-releasing action of quipazine and D-fenfluramine in rats. The role of brain serotonin in regulating prolactin (PRL) secretion has been investigated by studying the effect of quipazine and D-fenfluramine, two serotonin-like drugs, on plasma PRL levels under various experimental conditions. Quipazine (5, 10 and 20 mg/kg i.p.) and D-fenfluramine (5, 7.5 and 10 mg/kg i.p.) induced dose-related increases in plasma PRL levels in male rats. Intraventricular injection of 5,7-dihydroxytryptamine (5,7-DHT) or electrolytic lesion of the nucleus raphe medianus (MR), which caused a marked and selective depletion of hypothalamic serotonin levels, significantly reduced the PRL-releasing effect of both quipazine and D-fenfluramine. These results suggest that the effect of these drugs on PRL release is mediated through a serotonergic mechanism in the brain."}
{"id": "PMID:487125", "title": "L-[3H]Carnosine binding in the olfactory bulb. II. Biochemical and biological studies.", "content": "During the first 10 days after peripheral deafferentation of the mouse olfactory bulb stereoselective binding of L-[3H]carnosine declines markedly. The initial phase of this decline is due to a decrease in binding site stereoselectivity, which is then followed by a loss of assayable binding sites. The specificity of inhibition of L-[3H]carnosine binding by various peptides is also altered after denervation. Competitive inhibitors of carnosine binding become less potent after denervation, while analogues which are not competitive inhibitors remain equipotent before and after denervation. Several carnosine analogues that are normally poor inhibitors become more potent after denervation. Treatment of bulb membranes with trypsin, RNase and hyaluronidase, but not DNase or collagenase, resulted in significant alterations in carnosine binding. L-, but not D-carnosine, protected the binding site from trypsin digestion, and induced additional binding in bulb membranes in a dose-and temperature-dependent fashion. Preincubation of membranes with L-carnosine also led to the induction of additional carnosine binding in membranes from cerebral cortex, cerebellum and deafferentated bulbs but not from muscle. Bulbs from newborn mice contain about one-half of the adult levels of binding and no significant sex differences in carnosine binding were detected in bulbs from adult rats. L-[3H]carnosine binding was two-fold higher in the anterior compared to the posterior portion of the bulb, but there were no significant differences in binding of opiate, GABA, alpha-adrenergic, muscarinic cholinergic, benzodiazepine of glutamic acid receptor ligands.", "contents": "L-[3H]Carnosine binding in the olfactory bulb. II. Biochemical and biological studies. During the first 10 days after peripheral deafferentation of the mouse olfactory bulb stereoselective binding of L-[3H]carnosine declines markedly. The initial phase of this decline is due to a decrease in binding site stereoselectivity, which is then followed by a loss of assayable binding sites. The specificity of inhibition of L-[3H]carnosine binding by various peptides is also altered after denervation. Competitive inhibitors of carnosine binding become less potent after denervation, while analogues which are not competitive inhibitors remain equipotent before and after denervation. Several carnosine analogues that are normally poor inhibitors become more potent after denervation. Treatment of bulb membranes with trypsin, RNase and hyaluronidase, but not DNase or collagenase, resulted in significant alterations in carnosine binding. L-, but not D-carnosine, protected the binding site from trypsin digestion, and induced additional binding in bulb membranes in a dose-and temperature-dependent fashion. Preincubation of membranes with L-carnosine also led to the induction of additional carnosine binding in membranes from cerebral cortex, cerebellum and deafferentated bulbs but not from muscle. Bulbs from newborn mice contain about one-half of the adult levels of binding and no significant sex differences in carnosine binding were detected in bulbs from adult rats. L-[3H]carnosine binding was two-fold higher in the anterior compared to the posterior portion of the bulb, but there were no significant differences in binding of opiate, GABA, alpha-adrenergic, muscarinic cholinergic, benzodiazepine of glutamic acid receptor ligands."}
{"id": "PMID:487126", "title": "Ribosomal RNA in Mauthner axon: implications for a protein synthesizing machinery in the myelinated axon.", "content": "RNA was extracted from myelin-free Mauthner axons of the goldfish on a microscale and fractionated by microelectrophoresis. Microextracts showed the presence of nominal 26 SE, 18 SE, 5 SE and 4 SE components, which co-migrated with rRNA from fish brain. In addition, a non-ribosomal 15 SE component was present in axon microextracts, but not in RNA extracts of fish brain or of myelin sheath from Mauthner axon, indicating an unusual enrichment of a putative mRNA class. Evidence was presented to support the contention that axonal rRNA was not due to contamination from the myelin sheath. Possible reasons for the lack of ultrastructural evidence for axoplasmic ribosomes are discussed.", "contents": "Ribosomal RNA in Mauthner axon: implications for a protein synthesizing machinery in the myelinated axon. RNA was extracted from myelin-free Mauthner axons of the goldfish on a microscale and fractionated by microelectrophoresis. Microextracts showed the presence of nominal 26 SE, 18 SE, 5 SE and 4 SE components, which co-migrated with rRNA from fish brain. In addition, a non-ribosomal 15 SE component was present in axon microextracts, but not in RNA extracts of fish brain or of myelin sheath from Mauthner axon, indicating an unusual enrichment of a putative mRNA class. Evidence was presented to support the contention that axonal rRNA was not due to contamination from the myelin sheath. Possible reasons for the lack of ultrastructural evidence for axoplasmic ribosomes are discussed."}
{"id": "PMID:487127", "title": "Increase of large intramembranous particles in the presynaptic active zone after administration of 4-aminopyridine.", "content": "The effects of 4-aminopyridine (4-AP, 1 mg/kg i.v.) on the distribution of intramembranous particles in spinal motoneuronal synapses was examined in anesthetized (pentobarbital sodium, 50 mg/kg, i.p.) rats by freeze-etching electron microscopy. The particles were classified according to their size into small (4.2--10.5 nm in diameter) and large (10.5--18.9 nm in diameter). The active zone of the presynaptic membrane was identified on the basis of ultrastructural criteria. The large intramembranous particles in both protoplasmic (PF) and external fracture faces (EF) were aggregated more densely in the active zone as compared with its surroundings both in the control and 4-AP-treated group (P less than 0.001). The density of large particles in the PF rose from 300/sq.micron under the influence of 4-AP (P less than 0.001). The increased frequency of vesicle attachment sites at the presynaptic membrane (from 115/sq.micron in barbiturized controls to 150/sq.micron in 4-AP treated animals) seemed to be positively correlated with the increasing density of large particles. Based on these findings it is suggested that the large intramembranous particles of the presynaptic membrane may participate in the control of calcium fluxes which are essential to the transmitter release by exocytosis.", "contents": "Increase of large intramembranous particles in the presynaptic active zone after administration of 4-aminopyridine. The effects of 4-aminopyridine (4-AP, 1 mg/kg i.v.) on the distribution of intramembranous particles in spinal motoneuronal synapses was examined in anesthetized (pentobarbital sodium, 50 mg/kg, i.p.) rats by freeze-etching electron microscopy. The particles were classified according to their size into small (4.2--10.5 nm in diameter) and large (10.5--18.9 nm in diameter). The active zone of the presynaptic membrane was identified on the basis of ultrastructural criteria. The large intramembranous particles in both protoplasmic (PF) and external fracture faces (EF) were aggregated more densely in the active zone as compared with its surroundings both in the control and 4-AP-treated group (P less than 0.001). The density of large particles in the PF rose from 300/sq.micron under the influence of 4-AP (P less than 0.001). The increased frequency of vesicle attachment sites at the presynaptic membrane (from 115/sq.micron in barbiturized controls to 150/sq.micron in 4-AP treated animals) seemed to be positively correlated with the increasing density of large particles. Based on these findings it is suggested that the large intramembranous particles of the presynaptic membrane may participate in the control of calcium fluxes which are essential to the transmitter release by exocytosis."}
{"id": "PMID:487128", "title": "Involvement of catecholaminergic nerve fibers in angiotensin II-induced drinking in the Japanese quail, Coturnix coturnix japonica.", "content": "Monamine distribution in a septohypothalamic area was investigated in the Japanese quail using a histochemical fluorescence method. This area includes the subfornical organ (SFO) and the preoptic area (POA) which are inferred dipsogenic receptor sites for angiotensin II (AII) in the Japanese quail. Nerve fibers showing yellow-green fluorescence were found between the POA and the SFO. Thwy traversed from the POA to the SFO, and some fibers seemed to terminate on the neurons in the SFO. After a low dose of reserpine, a considerable number of fluorescent perikarya were found in the POA. These fibers and perikarya appeared to be of primary catecholamine judging from the fluorescence color. Following transection of these fibers, fluorescence disappeared from the fibers located on the SFO side of the transection plane, while it became a little more intense on the POA side. After transection, microinjection of AII into the POA was no longer effective in induction of drinking. On the other hand, sham operation or transection in areas other than between the POA and the SFO produced only minute changes in those fluorescent fibers and had little effect on the dipsogenic potency of AII injected into the POA. These results suggest that information of AII perceived at the POA is transferred to the SFO via those primary catecholamine-containing nerve fibers, which effect induced drinking.", "contents": "Involvement of catecholaminergic nerve fibers in angiotensin II-induced drinking in the Japanese quail, Coturnix coturnix japonica. Monamine distribution in a septohypothalamic area was investigated in the Japanese quail using a histochemical fluorescence method. This area includes the subfornical organ (SFO) and the preoptic area (POA) which are inferred dipsogenic receptor sites for angiotensin II (AII) in the Japanese quail. Nerve fibers showing yellow-green fluorescence were found between the POA and the SFO. Thwy traversed from the POA to the SFO, and some fibers seemed to terminate on the neurons in the SFO. After a low dose of reserpine, a considerable number of fluorescent perikarya were found in the POA. These fibers and perikarya appeared to be of primary catecholamine judging from the fluorescence color. Following transection of these fibers, fluorescence disappeared from the fibers located on the SFO side of the transection plane, while it became a little more intense on the POA side. After transection, microinjection of AII into the POA was no longer effective in induction of drinking. On the other hand, sham operation or transection in areas other than between the POA and the SFO produced only minute changes in those fluorescent fibers and had little effect on the dipsogenic potency of AII injected into the POA. These results suggest that information of AII perceived at the POA is transferred to the SFO via those primary catecholamine-containing nerve fibers, which effect induced drinking."}
{"id": "PMID:487129", "title": "Pineal N-acetyltransferase and hydroxyindole-O-methyltransferase: control by the retinohypothalamic tract and the suprachiasmatic nucleus.", "content": "The visual pathway and central neural structures involved in the photic and endogenous regulation of the activity of pineal N-acetyltransferase and hydroxyindole-O-methyltransferase were investigated. The results indicate that the visual pathway regulating both enzymes is the retinohypothalamic tract, and that the inferior accessory optic tract is clearly not involved in the regulation of hydroxyindole-O-methyltransferase activity, as has been previously thought. In addition, the suprachiasmatic nucleus was found to be necessary for the generation of a rhythm in N-acetyltransferase activity in blinded animals, and to be responsible for the tonic elevation of hydroxyindole-O-methyltransferase activity in blinded animals. Finally, it was concluded that the rapid and large daily changes in N-acetyltransferase activity seen in a normal lighting cycle and the much slower and smaller changes in hydroxyindole-O-methyltransferase activity seen only after weeks in constant lighting conditions are mediated by the same neural tract; the different time courses of the effects of environmental lighting may be explained on the basis of different intracellular regulatory mechanisms.", "contents": "Pineal N-acetyltransferase and hydroxyindole-O-methyltransferase: control by the retinohypothalamic tract and the suprachiasmatic nucleus. The visual pathway and central neural structures involved in the photic and endogenous regulation of the activity of pineal N-acetyltransferase and hydroxyindole-O-methyltransferase were investigated. The results indicate that the visual pathway regulating both enzymes is the retinohypothalamic tract, and that the inferior accessory optic tract is clearly not involved in the regulation of hydroxyindole-O-methyltransferase activity, as has been previously thought. In addition, the suprachiasmatic nucleus was found to be necessary for the generation of a rhythm in N-acetyltransferase activity in blinded animals, and to be responsible for the tonic elevation of hydroxyindole-O-methyltransferase activity in blinded animals. Finally, it was concluded that the rapid and large daily changes in N-acetyltransferase activity seen in a normal lighting cycle and the much slower and smaller changes in hydroxyindole-O-methyltransferase activity seen only after weeks in constant lighting conditions are mediated by the same neural tract; the different time courses of the effects of environmental lighting may be explained on the basis of different intracellular regulatory mechanisms."}
{"id": "PMID:487130", "title": "Radiometric assessment of experimental autoimmune encephalomyelitis in mice.", "content": "The cell-mediated inflammatory component of experimental autoimmune encephalomyelitis (EAE) in mice is measured by the radioisotopic technique. Mice are challenged with autologous spinal cord homogenate in Freund's complete adjuvant and at various time intervals after such immunization given [125I]5-iodo-2'-deoxyuridine which is incorporated into the mononuclear cell pool. The degree of cell-mediated inflammation is determined by radiometry of the brain and spinal cord tissues. Increased radiolabelling is detected in the brains 2 days prior to the onset of clinical signs of EAE; increased radioactivity of the spinal cord is concomitant with clinical signs. This technique is useful in staging the extent of EAE and may prove to be a powerful tool in studying cell-mediated reactions in other autoimmune diseases.", "contents": "Radiometric assessment of experimental autoimmune encephalomyelitis in mice. The cell-mediated inflammatory component of experimental autoimmune encephalomyelitis (EAE) in mice is measured by the radioisotopic technique. Mice are challenged with autologous spinal cord homogenate in Freund's complete adjuvant and at various time intervals after such immunization given [125I]5-iodo-2'-deoxyuridine which is incorporated into the mononuclear cell pool. The degree of cell-mediated inflammation is determined by radiometry of the brain and spinal cord tissues. Increased radiolabelling is detected in the brains 2 days prior to the onset of clinical signs of EAE; increased radioactivity of the spinal cord is concomitant with clinical signs. This technique is useful in staging the extent of EAE and may prove to be a powerful tool in studying cell-mediated reactions in other autoimmune diseases."}
{"id": "PMID:487136", "title": "Reflex oral activity in decerebrate rats of different age.", "content": "Rats decerebrated at different ages (4 days to greater than 100 days) were tested for a rhythmic reflex 'suckling' response and for a simple digastric reflex. The ease with which the 'suckling' response could be elicited decreased with increasing age; the time course of this change approximated to that of changing characteristics of the digastric reflex. The mean latency of the simple reflex response decreased non-linearly from 70 msec in the 4 day postpartum animal to 5 msec in the adult animal; the duration of the response also decreased from 25 msec to 10 msec but this change took place mainly between 8 and 15 days postpartum. The latency of the digastric response also shortened in animals decerebrated on day 4 and kept for 2 days. It was concluded that the observed changes in the digastric reflex occurred independently of encephalic structures.", "contents": "Reflex oral activity in decerebrate rats of different age. Rats decerebrated at different ages (4 days to greater than 100 days) were tested for a rhythmic reflex 'suckling' response and for a simple digastric reflex. The ease with which the 'suckling' response could be elicited decreased with increasing age; the time course of this change approximated to that of changing characteristics of the digastric reflex. The mean latency of the simple reflex response decreased non-linearly from 70 msec in the 4 day postpartum animal to 5 msec in the adult animal; the duration of the response also decreased from 25 msec to 10 msec but this change took place mainly between 8 and 15 days postpartum. The latency of the digastric response also shortened in animals decerebrated on day 4 and kept for 2 days. It was concluded that the observed changes in the digastric reflex occurred independently of encephalic structures."}
{"id": "PMID:487137", "title": "Partial purification and characterization of neutrophic substance affecting tetrodotoxin sensitivity of organ-cultured mouse muscle.", "content": "From mouse spinal cord homogenate, we isolated a trophic substance which reverses the post-denervation decrease in tetrodotoxin sensitivity of action potential in organ-cultured extensor digitorum longus muscle of mouse and characterized its physicochemical properties. The trophic substance was separated from macromolecules in homogenate by gel filtration on Biogel P2 column. The partially purified trophic substance was heat-stable, acid-stable and alkaline-labile. The trophic activity was destroyed by lyophilization at neutral pH but not at acidic pH. The trophic activity was abolished by incubation with pronase or leucine aminopeptidase, but not by trypsin, chymotrypsin, thermolysin or carboxypeptidase A. The trophic substance passed through an ultrafiltration membrane UM10 freely. A small part of the trophic activity passed through a UM2 or UM05, and the rest was retained on the membranes. The trophic substance adsorbed on CM-Sephadex at pH 7.2 but passed through DEAE-Sephadex at pH 8.4. These results suggest that the trophic substance regulating tetrodotoxin sensitivity of action potential in mouse skeletal muscle is a peptide with a rather low molecular weight of less than 10,000 and that while the N-terminus of the peptide is free, the C-terminus is probably blocked. This peptide differs from other trophic substances reported previously by other investigators.", "contents": "Partial purification and characterization of neutrophic substance affecting tetrodotoxin sensitivity of organ-cultured mouse muscle. From mouse spinal cord homogenate, we isolated a trophic substance which reverses the post-denervation decrease in tetrodotoxin sensitivity of action potential in organ-cultured extensor digitorum longus muscle of mouse and characterized its physicochemical properties. The trophic substance was separated from macromolecules in homogenate by gel filtration on Biogel P2 column. The partially purified trophic substance was heat-stable, acid-stable and alkaline-labile. The trophic activity was destroyed by lyophilization at neutral pH but not at acidic pH. The trophic activity was abolished by incubation with pronase or leucine aminopeptidase, but not by trypsin, chymotrypsin, thermolysin or carboxypeptidase A. The trophic substance passed through an ultrafiltration membrane UM10 freely. A small part of the trophic activity passed through a UM2 or UM05, and the rest was retained on the membranes. The trophic substance adsorbed on CM-Sephadex at pH 7.2 but passed through DEAE-Sephadex at pH 8.4. These results suggest that the trophic substance regulating tetrodotoxin sensitivity of action potential in mouse skeletal muscle is a peptide with a rather low molecular weight of less than 10,000 and that while the N-terminus of the peptide is free, the C-terminus is probably blocked. This peptide differs from other trophic substances reported previously by other investigators."}
{"id": "PMID:487138", "title": "Fate of presynaptic afferents to Purkinje cells in the adult nervous mutant mouse: a model to study presynaptic stabilization.", "content": "The hemispheric cerebellar cortex from 174- to 420-day-old nervous mice has been studied with Golgi, Cajal-reduced silver and electron microscopic techniques. In all mice, the existence of a continuous process of Purkinje cell death, indicated by the presence of few of these neurons in early stages of degeneration, has been established. The morphology of such degenerating cells, as well as the removal of the necrotic debris and the consecutive isolation of presynaptic fibers by glia are described. In the molecular layer, and in spite of the important Purkinje cell loss, numerous varicosities belonging to parallel fibers persist. They can be either synapsing on interneurons or concentrated in small clusters of 2-10 boutons covered by astrocytic processes. The synaptic investment of stellate and basket cells, as well as the length and the branching pattern of their dendritic fields are not significantly changed, besides the increase in afferent terminals deprived of their postsynaptic targets. Basket formations, as numerous as in control cerebellum, are forming a discontinuous row at the superficial third of the granular layer. Over 90% of these baskets are devoid of Purkinje cells, however, their ultrastructural features mimic those of the same terminals in control mice, with the exception that few of them can either establish heterologous synapses with granule cell dendrites, or develop gap junctions between them. The significance of the persistence of normal looking presynaptic elements long time after deprivation of their postsynaptic targets is discussed in relation to the role that function can play in the stabilization of synaptic contacts.", "contents": "Fate of presynaptic afferents to Purkinje cells in the adult nervous mutant mouse: a model to study presynaptic stabilization. The hemispheric cerebellar cortex from 174- to 420-day-old nervous mice has been studied with Golgi, Cajal-reduced silver and electron microscopic techniques. In all mice, the existence of a continuous process of Purkinje cell death, indicated by the presence of few of these neurons in early stages of degeneration, has been established. The morphology of such degenerating cells, as well as the removal of the necrotic debris and the consecutive isolation of presynaptic fibers by glia are described. In the molecular layer, and in spite of the important Purkinje cell loss, numerous varicosities belonging to parallel fibers persist. They can be either synapsing on interneurons or concentrated in small clusters of 2-10 boutons covered by astrocytic processes. The synaptic investment of stellate and basket cells, as well as the length and the branching pattern of their dendritic fields are not significantly changed, besides the increase in afferent terminals deprived of their postsynaptic targets. Basket formations, as numerous as in control cerebellum, are forming a discontinuous row at the superficial third of the granular layer. Over 90% of these baskets are devoid of Purkinje cells, however, their ultrastructural features mimic those of the same terminals in control mice, with the exception that few of them can either establish heterologous synapses with granule cell dendrites, or develop gap junctions between them. The significance of the persistence of normal looking presynaptic elements long time after deprivation of their postsynaptic targets is discussed in relation to the role that function can play in the stabilization of synaptic contacts."}
{"id": "PMID:487150", "title": "The effect of collateral sprouting on the density of innervation of normal target sites: implications for theories on the regulation of the size of developing synaptic domains.", "content": "The 'commissural' innervation of the dentate gyrus molecular layer has been analyzed in normal adult rats and in those in which the ipsilateral entorhinal cortex had been removed by aspiration at 14 days post-natal. This ablation severely deafferents the distal two-thirds of the molecular layer and induces 'sprouting' by the commissural afferents which are normally restricted to the more proximal dendritic zone. It was the objective of the present study to employ quantitative electron microscopy to determine (1) the extent of synaptic recovery in the deafferented field; (2) the magnitude of the contribution by the commissural fibers to the reinnervation of the deafferented field; and (3) if sprouting by the commissural projections causes a reduction in the density of the terminal field they generate in their normal target region. The synaptic density of the neonatally deafferented middle molecular layer was found to have returned to near control levels by adulthood. Degeneration studies performed in the adult revealed that commissural endings were located in equivalent numbers in the inner and middle molecular layers of rats in which the entorhinal cortex had been removed at 14 days post-natal; in normal rats (i.e. no neonatal surgery) the commissural terminals were found only in the inner molecular layer. Furthermore, and most importantly, the density of commissural terminals in the inner molecular layer was virtually identical in the 'sprouted' and control rats. Thus the tremendous areal expansion of the commissural terminal field which occurs after early deafferentation of the distal parts of the granule cell dendrites was not accompanied by any loss of input to the normal target of this afferent. Therefore, sprouting in this system represents an exaggeration of normal growth rather than a redistribution of a fixed population of endings. The relevance of these findings to theories concerned with the regulation of axonal growth and terminal proliferation during development is discussed.", "contents": "The effect of collateral sprouting on the density of innervation of normal target sites: implications for theories on the regulation of the size of developing synaptic domains. The 'commissural' innervation of the dentate gyrus molecular layer has been analyzed in normal adult rats and in those in which the ipsilateral entorhinal cortex had been removed by aspiration at 14 days post-natal. This ablation severely deafferents the distal two-thirds of the molecular layer and induces 'sprouting' by the commissural afferents which are normally restricted to the more proximal dendritic zone. It was the objective of the present study to employ quantitative electron microscopy to determine (1) the extent of synaptic recovery in the deafferented field; (2) the magnitude of the contribution by the commissural fibers to the reinnervation of the deafferented field; and (3) if sprouting by the commissural projections causes a reduction in the density of the terminal field they generate in their normal target region. The synaptic density of the neonatally deafferented middle molecular layer was found to have returned to near control levels by adulthood. Degeneration studies performed in the adult revealed that commissural endings were located in equivalent numbers in the inner and middle molecular layers of rats in which the entorhinal cortex had been removed at 14 days post-natal; in normal rats (i.e. no neonatal surgery) the commissural terminals were found only in the inner molecular layer. Furthermore, and most importantly, the density of commissural terminals in the inner molecular layer was virtually identical in the 'sprouted' and control rats. Thus the tremendous areal expansion of the commissural terminal field which occurs after early deafferentation of the distal parts of the granule cell dendrites was not accompanied by any loss of input to the normal target of this afferent. Therefore, sprouting in this system represents an exaggeration of normal growth rather than a redistribution of a fixed population of endings. The relevance of these findings to theories concerned with the regulation of axonal growth and terminal proliferation during development is discussed."}
{"id": "PMID:487151", "title": "Evidence for the existence of inhibitory postsynaptic potentials in dendrites and their functional significance in hippocampal pyramidal cells of adult rabbits.", "content": "(1) Hyperpolarizing potentials were studied in hippocampal pyramidal cells of normal, adult rabbits, anesthetized with Nembutal and, in addition, curarized. (2) Single shock stimulation of the fornix produced in these cells long-lasting hyperpolarizing potentials (400-2000 msec). As a rule, the early part of these potentials (about 200 msec from the onset) was influenced by the diffusion of Cl ions into the cell and current injections, whereas their late part was not. The early part exhibited impedance changes whereas the late part did not. From these results it was concluded that the former was the inhibitory postsynaptic potential (IPSP) occurring in the soma while the latter originated in the dendrites. Evidence was obtained suggesting that the latter was also a Cl-mediated IPSP. The hyperpolarization elicited in these cells by mesencephalic stimulation was similar in properties to the latter. (3) Disfacilitation in the dendrites and the functional significance of the dendritic IPSP were discussed.", "contents": "Evidence for the existence of inhibitory postsynaptic potentials in dendrites and their functional significance in hippocampal pyramidal cells of adult rabbits. (1) Hyperpolarizing potentials were studied in hippocampal pyramidal cells of normal, adult rabbits, anesthetized with Nembutal and, in addition, curarized. (2) Single shock stimulation of the fornix produced in these cells long-lasting hyperpolarizing potentials (400-2000 msec). As a rule, the early part of these potentials (about 200 msec from the onset) was influenced by the diffusion of Cl ions into the cell and current injections, whereas their late part was not. The early part exhibited impedance changes whereas the late part did not. From these results it was concluded that the former was the inhibitory postsynaptic potential (IPSP) occurring in the soma while the latter originated in the dendrites. Evidence was obtained suggesting that the latter was also a Cl-mediated IPSP. The hyperpolarization elicited in these cells by mesencephalic stimulation was similar in properties to the latter. (3) Disfacilitation in the dendrites and the functional significance of the dendritic IPSP were discussed."}
{"id": "PMID:487152", "title": "Sodium outflux and influx within nerve terminals from chronic epileptogenic foci.", "content": "The Na+-pump (oMpNa) or maximal sodium outflux inhibited by ouabain was studied in nerve terminals isolated from the primary and mirror epileptogenic foci of freezing lesions. In addition, the Na+ for Na+ exchange diffusion that is sensitive to ethacrynic acid and furosemide was also analyzed. oMpNa in control and epileptogenic states had similar and indistinguishable activation curves for K0 (extracellular K) in a sodium medium except for a two-fold difference in magnitude. However, the apparent affinity of the Na+-pump for K0 as measured by K1/2 was shifted to the right or decreased in epileptogenic foci (2 mM K0 compared to 0.5 mM in controls) when measured in a magnesium medium. A decrease in the apparent affinity for internal sodium was also observed. Of the total sodium outflux (67.7 nmol/mg/min), Na+ movements which are insensitive to ouabain and external K+ but stimulated by external Na+ and inhibited by furosemide and ethacrynic acid (20 nmol/mg/min or 32% of sodium outflux) represented the Na+ for Na+ exchange diffusion in nerve terminals. Na+ influx rising with increasing internal sodium in the presence of ouabain and blocked by furosemide represent the corresponding inward Na+ movement. No differences were observed between controls and epileptogenic states.", "contents": "Sodium outflux and influx within nerve terminals from chronic epileptogenic foci. The Na+-pump (oMpNa) or maximal sodium outflux inhibited by ouabain was studied in nerve terminals isolated from the primary and mirror epileptogenic foci of freezing lesions. In addition, the Na+ for Na+ exchange diffusion that is sensitive to ethacrynic acid and furosemide was also analyzed. oMpNa in control and epileptogenic states had similar and indistinguishable activation curves for K0 (extracellular K) in a sodium medium except for a two-fold difference in magnitude. However, the apparent affinity of the Na+-pump for K0 as measured by K1/2 was shifted to the right or decreased in epileptogenic foci (2 mM K0 compared to 0.5 mM in controls) when measured in a magnesium medium. A decrease in the apparent affinity for internal sodium was also observed. Of the total sodium outflux (67.7 nmol/mg/min), Na+ movements which are insensitive to ouabain and external K+ but stimulated by external Na+ and inhibited by furosemide and ethacrynic acid (20 nmol/mg/min or 32% of sodium outflux) represented the Na+ for Na+ exchange diffusion in nerve terminals. Na+ influx rising with increasing internal sodium in the presence of ouabain and blocked by furosemide represent the corresponding inward Na+ movement. No differences were observed between controls and epileptogenic states."}
{"id": "PMID:487153", "title": "The postnatal development of functional properties of central vestibular neurons in the rat.", "content": "The postnatal development of the responses of rat central vestibular neurons to horizontal angular acceleration was studied in the time and frequency domain. The resting discharge was very low and irregular during the first postnatal days, increased gradually and became more regular throughout the first month and reached adult values approximately by the end of the first month. The relative distribution of type I and type II units was the same in all age groups. Threshold for frequency increase to angular acceleration and sensitivity of unit responses became lower and higher, respectively, as time elapsed after birth. Adult values were reached approximately by the end of the first month. There was a slight tendency towards shorter time constants and smaller phase lags in one-month-old animals when compared with the younger animals. The results are discussed in conjunction with similar work performed in vestibular afferents and correlated with known morphological and behavioral studies.", "contents": "The postnatal development of functional properties of central vestibular neurons in the rat. The postnatal development of the responses of rat central vestibular neurons to horizontal angular acceleration was studied in the time and frequency domain. The resting discharge was very low and irregular during the first postnatal days, increased gradually and became more regular throughout the first month and reached adult values approximately by the end of the first month. The relative distribution of type I and type II units was the same in all age groups. Threshold for frequency increase to angular acceleration and sensitivity of unit responses became lower and higher, respectively, as time elapsed after birth. Adult values were reached approximately by the end of the first month. There was a slight tendency towards shorter time constants and smaller phase lags in one-month-old animals when compared with the younger animals. The results are discussed in conjunction with similar work performed in vestibular afferents and correlated with known morphological and behavioral studies."}
{"id": "PMID:487154", "title": "Synapses as associative memory elements in the hippocampal formation.", "content": "This report analyzes long term potentiation (LTP) and associative interactions between synapses of the ipsilateral and crossed entorhinal cortical (EC) pathways to the dentate gyrus (DG). In the anesthetized rat, conditioning stimulation to one EC-DG pathway reliably elicits LTP at the ipsilateral synapses, while the synapses of the collateral, crossed pathway to the contralateral DG do not exhibit LTP. Furthermore, in the DG ipsilateral to the conditioning stimulation the convergent crossed pathway from the contralateral side, which had not been itself conditioned, failed to exhibit heterosynaptic LTP. These results are consistent with a specific 'synaptic' localization of the changes responsible for LTP, and suggest that some critical number of synapses must be activated in order to observe LTP. While the crossed EC-DG projection never exhibited LTP when conditioned alone, the crossed input could be potentiated under certain circumstances. Specifically, paired conditioning of ipsi- and contralateral inputs by nearly simultaneous conditioning stimulation of the EC bilaterally results in LTP in the crossed system. Furthermore, this associatively induced LTP of the crossed system can be reversed by subsequent conditioning of the ipsilateral system alone. Successive potentiating and depotentiating sequences are possible using paired and non-paired stimulation procedures even after lesions which prevent neural loops through the EC. The results are interpreted as evidence for a 'Hebb' type synapse which has the capability for erasure. This synaptic type is not appropriate for classical conditioning without appendant circuitry, but is suited for other forms of associative learning.", "contents": "Synapses as associative memory elements in the hippocampal formation. This report analyzes long term potentiation (LTP) and associative interactions between synapses of the ipsilateral and crossed entorhinal cortical (EC) pathways to the dentate gyrus (DG). In the anesthetized rat, conditioning stimulation to one EC-DG pathway reliably elicits LTP at the ipsilateral synapses, while the synapses of the collateral, crossed pathway to the contralateral DG do not exhibit LTP. Furthermore, in the DG ipsilateral to the conditioning stimulation the convergent crossed pathway from the contralateral side, which had not been itself conditioned, failed to exhibit heterosynaptic LTP. These results are consistent with a specific 'synaptic' localization of the changes responsible for LTP, and suggest that some critical number of synapses must be activated in order to observe LTP. While the crossed EC-DG projection never exhibited LTP when conditioned alone, the crossed input could be potentiated under certain circumstances. Specifically, paired conditioning of ipsi- and contralateral inputs by nearly simultaneous conditioning stimulation of the EC bilaterally results in LTP in the crossed system. Furthermore, this associatively induced LTP of the crossed system can be reversed by subsequent conditioning of the ipsilateral system alone. Successive potentiating and depotentiating sequences are possible using paired and non-paired stimulation procedures even after lesions which prevent neural loops through the EC. The results are interpreted as evidence for a 'Hebb' type synapse which has the capability for erasure. This synaptic type is not appropriate for classical conditioning without appendant circuitry, but is suited for other forms of associative learning."}
{"id": "PMID:487155", "title": "Release of immunoreactive alpha-MSH by synaptosome-enriched fractions of homogenates of hypothalami.", "content": "Immunoreactive alpha-melanocyte-stimulating hormone (alpha-MSH) was found to be concentrated in a synaptosome-enriched fraction prepared by differential centrifugation of rat hypothalamic homogenates. The release of the hormone from this preparation was investigated. After incubation, the synaptosomes were isolated by ultrafiltration and alpha-MSH in the ultrafiltrate was determined by radioimmunoassay. Particle-bound alpha-MSH, recovered by extraction with acid ethanol, and alpha-MSH released from the synaptosome preparation, were immunologically similar to synthetic alpha-MSH and had an accompanying melanotropic activity. Less than 10% of the particle-bound alpha-MSH was released during incubation in 0.32 M sucrose. However, in the presence of 2 mM Ca2+, alpha-MSH release increased with increasing concentrations (30-150 mM) of K+. The stimulatory effect of 60 mM K+ was complete within 2 min and was potentiated by increasing Ca2+ concentrations over the range of 0 to 2 mM. K+-induced release of alpha-MSH was independent of temperature from 1 to 30 degrees C, and neither glucose (10 mM) nor dopamine (10(-10)-10(-2) M) had any effect on the release of the peptide. It is concluded that a synaptosome-enriched fraction from the hypothalamus contains a releasable pool of immunoreactive alpha-MSH that is mobilized by depolarizing concentrations of K+ in a Ca2+-dependent manner.", "contents": "Release of immunoreactive alpha-MSH by synaptosome-enriched fractions of homogenates of hypothalami. Immunoreactive alpha-melanocyte-stimulating hormone (alpha-MSH) was found to be concentrated in a synaptosome-enriched fraction prepared by differential centrifugation of rat hypothalamic homogenates. The release of the hormone from this preparation was investigated. After incubation, the synaptosomes were isolated by ultrafiltration and alpha-MSH in the ultrafiltrate was determined by radioimmunoassay. Particle-bound alpha-MSH, recovered by extraction with acid ethanol, and alpha-MSH released from the synaptosome preparation, were immunologically similar to synthetic alpha-MSH and had an accompanying melanotropic activity. Less than 10% of the particle-bound alpha-MSH was released during incubation in 0.32 M sucrose. However, in the presence of 2 mM Ca2+, alpha-MSH release increased with increasing concentrations (30-150 mM) of K+. The stimulatory effect of 60 mM K+ was complete within 2 min and was potentiated by increasing Ca2+ concentrations over the range of 0 to 2 mM. K+-induced release of alpha-MSH was independent of temperature from 1 to 30 degrees C, and neither glucose (10 mM) nor dopamine (10(-10)-10(-2) M) had any effect on the release of the peptide. It is concluded that a synaptosome-enriched fraction from the hypothalamus contains a releasable pool of immunoreactive alpha-MSH that is mobilized by depolarizing concentrations of K+ in a Ca2+-dependent manner."}
{"id": "PMID:487156", "title": "Symmetric bilateral changes in dopamine release from the caudate nuclei of the cat induced by unilateral nigral application of glycine and GABA-related compounds.", "content": "The release of [3H]DA synthesized from [3H]tyrosine was estimated in the two caudate nuclei (CN) during the unilateral nigral application of glycine and GABA-related compounds in 'enc\u00e9phale isol\u00e9' cats using push-pull cannulae. Glycine (10(-5) M) reduced the release of [3H]DA in both CN and these effects were antagonized by strychnine (10(-5) M). A decrease in [3H]DA release was also seen in both CN during the unilateral nigral application of diazepam (10(-5) M). In contrast, muscimol (10(-6) M) and GABA (10(-5) M) stimulated [3H]DA release on both sides. The effect of GABA was blocked by picrotoxin (10(-5) M). Picrotoxin alone stimulated the release of [3H]DA in the ipsilateral CN and was without effect in the contralateral side. Bicuculline (10(-5) M) stimulated [3H]DA release only in the contralateral CN. A symmetric increase in [3H]DA release in both CN was also observed during the unilateral nigral application of potassium (30 mM). A model involving a facilitatory polysynaptic pathway originating from the substantia nigra (SN) and acting presynaptically on ther terminals of the contralateral DA neurons is proposed to explain the changes in [3H]DA release induced in the contralateral CN in these various situations. The results are discussed taking into account previous data on the reciprocal control of the two dopaminergic pathways induced by the unilateral nigral application of dopaminergic drugs.", "contents": "Symmetric bilateral changes in dopamine release from the caudate nuclei of the cat induced by unilateral nigral application of glycine and GABA-related compounds. The release of [3H]DA synthesized from [3H]tyrosine was estimated in the two caudate nuclei (CN) during the unilateral nigral application of glycine and GABA-related compounds in 'enc\u00e9phale isol\u00e9' cats using push-pull cannulae. Glycine (10(-5) M) reduced the release of [3H]DA in both CN and these effects were antagonized by strychnine (10(-5) M). A decrease in [3H]DA release was also seen in both CN during the unilateral nigral application of diazepam (10(-5) M). In contrast, muscimol (10(-6) M) and GABA (10(-5) M) stimulated [3H]DA release on both sides. The effect of GABA was blocked by picrotoxin (10(-5) M). Picrotoxin alone stimulated the release of [3H]DA in the ipsilateral CN and was without effect in the contralateral side. Bicuculline (10(-5) M) stimulated [3H]DA release only in the contralateral CN. A symmetric increase in [3H]DA release in both CN was also observed during the unilateral nigral application of potassium (30 mM). A model involving a facilitatory polysynaptic pathway originating from the substantia nigra (SN) and acting presynaptically on ther terminals of the contralateral DA neurons is proposed to explain the changes in [3H]DA release induced in the contralateral CN in these various situations. The results are discussed taking into account previous data on the reciprocal control of the two dopaminergic pathways induced by the unilateral nigral application of dopaminergic drugs."}
{"id": "PMID:487157", "title": "The effect of intracerebral ouabain administration on the composition of edema fluid isolated from cats with cold-induced brain edema.", "content": "Brain edema fluid was collected from cats with a freezing lesion in the left parietal cortex by the insertion into the brain of needles containing nylon wicks and connected to polyethylene tubes. The edema fluid samples which accumulated in the polyethylene tubes were regularly analyzed for Na+ and K+ content, colloid osmotic pressure, lactate dehydrogenase and creatine phosphokinase activity, and 99mTc-albumin radioactivity; the albumin tracer being introduced intravenously at the time of cold-injury. One series of cats received an intracerebral injection of ouabain solution, the control series an intracerebral injection of saline, at 100 min after the cold-injury. The ouabain injection was followed by an increase of K+ content, LDH and CPK activities but a decrease of Na+ concentration in the edema fluid, attributable to a concentration of solutes in the edema fluid as presumably water and Na+ were shifted into the cells and hence the extracellular space was reduced.", "contents": "The effect of intracerebral ouabain administration on the composition of edema fluid isolated from cats with cold-induced brain edema. Brain edema fluid was collected from cats with a freezing lesion in the left parietal cortex by the insertion into the brain of needles containing nylon wicks and connected to polyethylene tubes. The edema fluid samples which accumulated in the polyethylene tubes were regularly analyzed for Na+ and K+ content, colloid osmotic pressure, lactate dehydrogenase and creatine phosphokinase activity, and 99mTc-albumin radioactivity; the albumin tracer being introduced intravenously at the time of cold-injury. One series of cats received an intracerebral injection of ouabain solution, the control series an intracerebral injection of saline, at 100 min after the cold-injury. The ouabain injection was followed by an increase of K+ content, LDH and CPK activities but a decrease of Na+ concentration in the edema fluid, attributable to a concentration of solutes in the edema fluid as presumably water and Na+ were shifted into the cells and hence the extracellular space was reduced."}
{"id": "PMID:487158", "title": "Changes in brain and spinal tryptophan and 5-hydroxyindoleacetic acid levels following acute morphine administration in normal and arthritic rats.", "content": "The effects of morphine (10 mg/kg/s.c.) on tryptophan (TRP), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) levels were studied in normal and arthritic rats. (1) In normal rats morphine induced a discrete but significant increase of 5-HIAA levels in the forebrain and the spinal cord. (2) By contrast, in rats suffering from experimentally induced arthritis large modifications were apparent. The basal levels of TRP, 5-HT and 5-HIAA were significantly higher than in normal rats. Morphine induced clear increases of 5-HIAA and TRP in the forebrain, the brain stem and the spinal cord, without any modification of 5-HT. The effects were dose-dependent and suppressed by naloxone (1 mg/kg/i.m.). Statistical analysis clearly revealed that arthritic rats were much more sensitive to morphine. The results support the hypothesis of an activation of a 5-HT descending pathway by morphine which parallels the activation of the ascending pathway previously demonstrated by several authors and confirmed here.", "contents": "Changes in brain and spinal tryptophan and 5-hydroxyindoleacetic acid levels following acute morphine administration in normal and arthritic rats. The effects of morphine (10 mg/kg/s.c.) on tryptophan (TRP), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) levels were studied in normal and arthritic rats. (1) In normal rats morphine induced a discrete but significant increase of 5-HIAA levels in the forebrain and the spinal cord. (2) By contrast, in rats suffering from experimentally induced arthritis large modifications were apparent. The basal levels of TRP, 5-HT and 5-HIAA were significantly higher than in normal rats. Morphine induced clear increases of 5-HIAA and TRP in the forebrain, the brain stem and the spinal cord, without any modification of 5-HT. The effects were dose-dependent and suppressed by naloxone (1 mg/kg/i.m.). Statistical analysis clearly revealed that arthritic rats were much more sensitive to morphine. The results support the hypothesis of an activation of a 5-HT descending pathway by morphine which parallels the activation of the ascending pathway previously demonstrated by several authors and confirmed here."}
{"id": "PMID:487159", "title": "Effect of oxytocin and vasopressin on memory consolidation: sites of action and catecholaminergic correlates after local microinjection into limbic-midbrain structures.", "content": "The effects of local postlearning microinjections of arginine-vasopressin (AVP) and oxytocin (OXT) on one-trial learning passive avoidance behavior and the influence of AVP on alpha-MPT-induced disappearance of norepinephrine (NE) and dopamine (DA) in discrete brain regions have been studied in the rat. OXT injected bilaterally in the hippocampal dentate gyrus (25-25 pg) or in the midbrain dorsal raphe nucleus (50 pg) significantly attenuated passive avoidance behavior. Facilitation of passive avoidance behavior was observed when the peptide was injected into the dorsal septal nucleus. AVP facilitated passive avoidance behavior when administered into the hippocampal dentate gyrus, dorsal raphe nucleus or dorsal septal nucleus. Injection of either neuropeptides into the central amygdaloid nucleus appeared to be ineffective. One week after the behavioral experiments a repeated injection of AVP into the hippocampal dentate gyrus increased the disappearance of NE in the dentate gyrus and in the nucleus ruber. An injection into the dorsal septal nuclei decreased the NE disappearance in the dorsal septal nucleus itself and increased it in the nucleus ruber. Injection in the dorsal raphe nucleus led to an increase in the disappearance of DA in the locus coeruleus and in the nucleus ruber. It is concluded that memory consolidation can be oppositely influenced by local application of minute amounts of either OXT or AVP into certain limbic-midbrain structures, suggesting an involvement of these brain regions in the memory effects of these peptides. Modulation of catecholamine turnover in specific brain areas after AVP administration may be related to this behavioral effect.", "contents": "Effect of oxytocin and vasopressin on memory consolidation: sites of action and catecholaminergic correlates after local microinjection into limbic-midbrain structures. The effects of local postlearning microinjections of arginine-vasopressin (AVP) and oxytocin (OXT) on one-trial learning passive avoidance behavior and the influence of AVP on alpha-MPT-induced disappearance of norepinephrine (NE) and dopamine (DA) in discrete brain regions have been studied in the rat. OXT injected bilaterally in the hippocampal dentate gyrus (25-25 pg) or in the midbrain dorsal raphe nucleus (50 pg) significantly attenuated passive avoidance behavior. Facilitation of passive avoidance behavior was observed when the peptide was injected into the dorsal septal nucleus. AVP facilitated passive avoidance behavior when administered into the hippocampal dentate gyrus, dorsal raphe nucleus or dorsal septal nucleus. Injection of either neuropeptides into the central amygdaloid nucleus appeared to be ineffective. One week after the behavioral experiments a repeated injection of AVP into the hippocampal dentate gyrus increased the disappearance of NE in the dentate gyrus and in the nucleus ruber. An injection into the dorsal septal nuclei decreased the NE disappearance in the dorsal septal nucleus itself and increased it in the nucleus ruber. Injection in the dorsal raphe nucleus led to an increase in the disappearance of DA in the locus coeruleus and in the nucleus ruber. It is concluded that memory consolidation can be oppositely influenced by local application of minute amounts of either OXT or AVP into certain limbic-midbrain structures, suggesting an involvement of these brain regions in the memory effects of these peptides. Modulation of catecholamine turnover in specific brain areas after AVP administration may be related to this behavioral effect."}
{"id": "PMID:487160", "title": "The effects of acute and chronic morphine treatment on the process of facial nerve regeneration.", "content": "Prominent cellular responses to axonal interruption include enhanced synthesis of RNA and protein in the neuronal perikaryon, and proliferation of reactive Schwann cells. Since morphine has been shown to significantly depress cellular metabolism, we examined its effect on these and other reparative responses underlying nerve fiber regeneration. Rat facial nerve trunks from saline, acute morphine, and continuous morphine-treated animals were examined by light and electron microscopy at 3, 7 and 14 days after crush injury. The number of axonal sprouts/unit area and the diameters of regenerating axons were quantified at each survival interval. Both saline-treated and acute morphine-treated facial nerves demonstrated myelin degradation and Schwann cell hypertrophy (at 3 days post-axotomy), sprout outgrowth (at 7 days) and axon maturation and myelination (at 14 days). In the chronic morphine-treated animals, a retardation of the regenerative process was evident. Axon sprout outgrowth and axonal diameters were reduced at 3 and 7 days post-axotomy. In treated 14-day animals, axon diameters were normal; however, significantly fewer axon profiles/unit area were observed. After chronic morphine exposure, Schwann cell hypertrophy and proliferation, as well as myelin debris removal, were inhibited at all survival periods.", "contents": "The effects of acute and chronic morphine treatment on the process of facial nerve regeneration. Prominent cellular responses to axonal interruption include enhanced synthesis of RNA and protein in the neuronal perikaryon, and proliferation of reactive Schwann cells. Since morphine has been shown to significantly depress cellular metabolism, we examined its effect on these and other reparative responses underlying nerve fiber regeneration. Rat facial nerve trunks from saline, acute morphine, and continuous morphine-treated animals were examined by light and electron microscopy at 3, 7 and 14 days after crush injury. The number of axonal sprouts/unit area and the diameters of regenerating axons were quantified at each survival interval. Both saline-treated and acute morphine-treated facial nerves demonstrated myelin degradation and Schwann cell hypertrophy (at 3 days post-axotomy), sprout outgrowth (at 7 days) and axon maturation and myelination (at 14 days). In the chronic morphine-treated animals, a retardation of the regenerative process was evident. Axon sprout outgrowth and axonal diameters were reduced at 3 and 7 days post-axotomy. In treated 14-day animals, axon diameters were normal; however, significantly fewer axon profiles/unit area were observed. After chronic morphine exposure, Schwann cell hypertrophy and proliferation, as well as myelin debris removal, were inhibited at all survival periods."}
{"id": "PMID:487168", "title": "Effects of suckling on serum prolactin levels and catecholamine concentrations and turnover in discrete brain regions.", "content": "The effects of suckling on serum prolactin levels and catecholamine concentrations and turnover were examined in several discrete brain regions. Turnover rates were assessed by using the synthesis inhibitor alpha-methyltyrosine (alpha-MT) in combination with microdissection techniques for the removal of individual brain regions and sensitive radioenzymatic assays for norepinephrine (NE) and dopaime (DA). Prolactin secretion was induced by mothers experiencing 6 h of pup removal with subsequent pup replacement. Suckling or the administration of alpha-MT to mothers resulted in a marked increase in circulating titers of prolactin. A decrease in steady-state NE concentrations in the anterior hypothalamus and a decrease in steady-state DA concentrations in the ventromedial nucleus were noted in suckled mothers. The comparison of relative rates of NE depletion after alpha-MT treatment revealed a suckling-induced increase in turnover in the ventromedial nucleus and a suckling-induced decrease in turnover in the anterior hypothalamus. Neither suckling nor alpha-MT treatment produced any changes in NE or DA turnover rates in the arcuate nucleus or median eminence. These findings demonstrate that suckling-induced activation of prolactin results in changes in noradrenergic processes in the ventromedial and anterior hypothalamic nuclei. This suggests an involvement of noradrenergic systems in suckling-induced prolactin release.", "contents": "Effects of suckling on serum prolactin levels and catecholamine concentrations and turnover in discrete brain regions. The effects of suckling on serum prolactin levels and catecholamine concentrations and turnover were examined in several discrete brain regions. Turnover rates were assessed by using the synthesis inhibitor alpha-methyltyrosine (alpha-MT) in combination with microdissection techniques for the removal of individual brain regions and sensitive radioenzymatic assays for norepinephrine (NE) and dopaime (DA). Prolactin secretion was induced by mothers experiencing 6 h of pup removal with subsequent pup replacement. Suckling or the administration of alpha-MT to mothers resulted in a marked increase in circulating titers of prolactin. A decrease in steady-state NE concentrations in the anterior hypothalamus and a decrease in steady-state DA concentrations in the ventromedial nucleus were noted in suckled mothers. The comparison of relative rates of NE depletion after alpha-MT treatment revealed a suckling-induced increase in turnover in the ventromedial nucleus and a suckling-induced decrease in turnover in the anterior hypothalamus. Neither suckling nor alpha-MT treatment produced any changes in NE or DA turnover rates in the arcuate nucleus or median eminence. These findings demonstrate that suckling-induced activation of prolactin results in changes in noradrenergic processes in the ventromedial and anterior hypothalamic nuclei. This suggests an involvement of noradrenergic systems in suckling-induced prolactin release."}
{"id": "PMID:487169", "title": "Opioid peptides excite pyramidal neurons and evoke epileptiform activity in hippocampal transplants in oculo.", "content": "The effect of opiate peptide administration on the electrical activity of intraocular hippocampal transplants was studied. Similar to observations in situ, the administration of beta-endorphin or methionine enkephalin produces a concentration-dependent increase in the firing rate of identified pyramidal neurons within hippocampal formation transplants. In addition, these peptides elicit a profound increase in 'EEG' amplitude, which ultimately develops into epileptiform activity. The ability of naloxone to either reverse or prevent the peptide-induced changes in both single unit and EEG activity supports the hypothesis that the excitatory response of the hippocampus to opioid peptides is mediated via an opiate receptor. The results of this study also suggest that the excitatory response to the opiate peptides in hippocampus is the result of alterations in intrinsic neuronal circuitry and is not dependent upon extra-hippocampal afferents.", "contents": "Opioid peptides excite pyramidal neurons and evoke epileptiform activity in hippocampal transplants in oculo. The effect of opiate peptide administration on the electrical activity of intraocular hippocampal transplants was studied. Similar to observations in situ, the administration of beta-endorphin or methionine enkephalin produces a concentration-dependent increase in the firing rate of identified pyramidal neurons within hippocampal formation transplants. In addition, these peptides elicit a profound increase in 'EEG' amplitude, which ultimately develops into epileptiform activity. The ability of naloxone to either reverse or prevent the peptide-induced changes in both single unit and EEG activity supports the hypothesis that the excitatory response of the hippocampus to opioid peptides is mediated via an opiate receptor. The results of this study also suggest that the excitatory response to the opiate peptides in hippocampus is the result of alterations in intrinsic neuronal circuitry and is not dependent upon extra-hippocampal afferents."}
{"id": "PMID:487181", "title": "The fine structure of the feline superior vestibular nucleus: identification and synaptology of the primary vestibular afferents.", "content": "The superior vestibular nucleus of the cat and its primary vestibular efferents were examined by light and electron microscopy. The primary vestibular afferents branch within the nucleus in a sheet-like pattern, in the transverse plane. The dendritic fields of many secondary neurons are shaped like discs and are also oriented in the transverse plane. This relation between the primary afferents and dendritic fields may be relevant to the convergence of primary afferents innervating particular endorgans onto secondary neurons. Synaptic boutons in the SV were divided into 3 putative types on the basis of the size and shape of their synaptic vesicles. The primary afferent bouton was identified by comparing the SV of the two sides after unilateral lesions of the vestibular ganglion. Its boutons contain round vesicles of 40 nm average diameter and are associated with prominent postsynaptic densities; the two other putative bouton types contain smaller, round vesicles, and pleomorphic vesicles. The primary afferent boutons largely contact proximal dendrites, their appendages, and cell somata of the secondary neurons. In animals receiving unilateral lesions of the vestibular ganglion and allowed to survive long enough for the primary afferent boutons to disappear (5--6 days), there occurs in the denervated as compared to normal SV: (1) a decrease in the fraction of the somal surface of the secondary neurons covered by boutons with small round vesicles; and (2) a decrease in the ratio: number of boutons with small round vesicles to number of boutons with pleomorphic vesicles. In addition, there appears on the lesioned side a new group of boutons with pleomorphic vesicles smaller than those in boutons from the control side. These observations suggest plastic changes in response to deafferentation, and may be related to the marked behavioral recovery which occurs within a few days after lesion of the vestibular ganglion.", "contents": "The fine structure of the feline superior vestibular nucleus: identification and synaptology of the primary vestibular afferents. The superior vestibular nucleus of the cat and its primary vestibular efferents were examined by light and electron microscopy. The primary vestibular afferents branch within the nucleus in a sheet-like pattern, in the transverse plane. The dendritic fields of many secondary neurons are shaped like discs and are also oriented in the transverse plane. This relation between the primary afferents and dendritic fields may be relevant to the convergence of primary afferents innervating particular endorgans onto secondary neurons. Synaptic boutons in the SV were divided into 3 putative types on the basis of the size and shape of their synaptic vesicles. The primary afferent bouton was identified by comparing the SV of the two sides after unilateral lesions of the vestibular ganglion. Its boutons contain round vesicles of 40 nm average diameter and are associated with prominent postsynaptic densities; the two other putative bouton types contain smaller, round vesicles, and pleomorphic vesicles. The primary afferent boutons largely contact proximal dendrites, their appendages, and cell somata of the secondary neurons. In animals receiving unilateral lesions of the vestibular ganglion and allowed to survive long enough for the primary afferent boutons to disappear (5--6 days), there occurs in the denervated as compared to normal SV: (1) a decrease in the fraction of the somal surface of the secondary neurons covered by boutons with small round vesicles; and (2) a decrease in the ratio: number of boutons with small round vesicles to number of boutons with pleomorphic vesicles. In addition, there appears on the lesioned side a new group of boutons with pleomorphic vesicles smaller than those in boutons from the control side. These observations suggest plastic changes in response to deafferentation, and may be related to the marked behavioral recovery which occurs within a few days after lesion of the vestibular ganglion."}
{"id": "PMID:487182", "title": "The bilaminar and banded distribution of the callosal terminals in the posterior neocortex of the rat.", "content": "After callosal sectioning, the callosal connections of the posterior neocortex of the rat cerebral hemisphere were demonstrated using the Fink-Heimer technique. Serial frozen sections of the whole brains were cut in transverse, horizontal, and tangential planes. In tissue sections, degenerating terminals were concentrated in two distinct laminae within the depth of the cortex. In addition the terminals had a patchy distribution. The degeneration was marked on projection drawings of serially arranged sections, and subsequent reconstruction showed the terminal degeneration to be distributed in bands. Five dorsoventrally oriented bands of terminals were present in areas 39, 41 and 36 collectively, and a rostrocaudal band in area 20. In area 17 terminations were apparently absent except at its borders with areas 18, 18a and 7. The degenerating callosal terminals within these areas produced a circumferential band around area 17. The findings are discussed with respect to the significance of these patterns of corticocortical connections.", "contents": "The bilaminar and banded distribution of the callosal terminals in the posterior neocortex of the rat. After callosal sectioning, the callosal connections of the posterior neocortex of the rat cerebral hemisphere were demonstrated using the Fink-Heimer technique. Serial frozen sections of the whole brains were cut in transverse, horizontal, and tangential planes. In tissue sections, degenerating terminals were concentrated in two distinct laminae within the depth of the cortex. In addition the terminals had a patchy distribution. The degeneration was marked on projection drawings of serially arranged sections, and subsequent reconstruction showed the terminal degeneration to be distributed in bands. Five dorsoventrally oriented bands of terminals were present in areas 39, 41 and 36 collectively, and a rostrocaudal band in area 20. In area 17 terminations were apparently absent except at its borders with areas 18, 18a and 7. The degenerating callosal terminals within these areas produced a circumferential band around area 17. The findings are discussed with respect to the significance of these patterns of corticocortical connections."}
{"id": "PMID:487183", "title": "Orthodromic activation of hippocampal CA1 region of the rat.", "content": "(1) The posterior alveus (PA), the anterior alveus (AA) and the Schaffer collaterals (SCH) evoked field potential components which were organized as parasagittal strips of various widths. Spatially continuous and interactive lamellae are suggested. (2) By correlation with unit activities, the early postsynaptic components evoked by PA, AA and SCH were inferred to be extracellular excitatory postsynaptic potentials (EPSPs) and the late, long-duration components, the inhibitory postsynaptic potentials (IPSPs). The hypothesis that interneurons as well as pyramidal cells generate the field is proposed and discussed. (3) One- and two-dimensional profiles of deep evoked potentials and current source-sink analysis revealed excitatory synapses in stratum oriens for the PA and AA inputs and in stratum radiatum for the SCH input. The late dipole field evoked by PA and AA possessed current sources in strata radiatum and pyramidale, the sites of the inhibitory synapses. The late dipole field evoked by SCH had another component possibly generated by recurrent activity, afterpotentials or relayed activity through CA3.", "contents": "Orthodromic activation of hippocampal CA1 region of the rat. (1) The posterior alveus (PA), the anterior alveus (AA) and the Schaffer collaterals (SCH) evoked field potential components which were organized as parasagittal strips of various widths. Spatially continuous and interactive lamellae are suggested. (2) By correlation with unit activities, the early postsynaptic components evoked by PA, AA and SCH were inferred to be extracellular excitatory postsynaptic potentials (EPSPs) and the late, long-duration components, the inhibitory postsynaptic potentials (IPSPs). The hypothesis that interneurons as well as pyramidal cells generate the field is proposed and discussed. (3) One- and two-dimensional profiles of deep evoked potentials and current source-sink analysis revealed excitatory synapses in stratum oriens for the PA and AA inputs and in stratum radiatum for the SCH input. The late dipole field evoked by PA and AA possessed current sources in strata radiatum and pyramidale, the sites of the inhibitory synapses. The late dipole field evoked by SCH had another component possibly generated by recurrent activity, afterpotentials or relayed activity through CA3."}
{"id": "PMID:487184", "title": "Functional effects of lesion-induced plasticity: long term potentiation in formal and lesion-induced temporodentate connections.", "content": "The crossed temporodentate pathway from the entorhinal cortex of one hemisphere which proliferates in response to a contralateral entorhinal lesion in adult rats was analyzed for its ability to exhibit long term potentiation of synaptic efficacy similar to that which occurs in the normal ipsilateral temporodentate pathway. It was found that while the small synaptic response evoked by contralateral entorhinal cortical stimulation in normal rats does not undergo long term potentiation, after unilateral entorhinal lesions and proliferation of the crossed temporodentate pathway, the crossed pathway acquires a capacity for potentiation of synaptic action which qualitatively resembles that of the normal ipsilateral temporodentate circuit. However, despite the potentiation of synaptic drive, no long term enhancement of cell discharge was observed in the re-innervated dentate gyrus even through potentiation of this parameter was very prominent in the ipsilateral pathway. Mechanisms are discussed by which a previously non-potentiating pathway may acquire, as a consequence of lesion-induced sprouting, an ability to undergo long term potentiation of synaptic efficacy in a fasion similar to the ablated pathway. Reasons for the failure to observe potentiation of cell firing are also considered.", "contents": "Functional effects of lesion-induced plasticity: long term potentiation in formal and lesion-induced temporodentate connections. The crossed temporodentate pathway from the entorhinal cortex of one hemisphere which proliferates in response to a contralateral entorhinal lesion in adult rats was analyzed for its ability to exhibit long term potentiation of synaptic efficacy similar to that which occurs in the normal ipsilateral temporodentate pathway. It was found that while the small synaptic response evoked by contralateral entorhinal cortical stimulation in normal rats does not undergo long term potentiation, after unilateral entorhinal lesions and proliferation of the crossed temporodentate pathway, the crossed pathway acquires a capacity for potentiation of synaptic action which qualitatively resembles that of the normal ipsilateral temporodentate circuit. However, despite the potentiation of synaptic drive, no long term enhancement of cell discharge was observed in the re-innervated dentate gyrus even through potentiation of this parameter was very prominent in the ipsilateral pathway. Mechanisms are discussed by which a previously non-potentiating pathway may acquire, as a consequence of lesion-induced sprouting, an ability to undergo long term potentiation of synaptic efficacy in a fasion similar to the ablated pathway. Reasons for the failure to observe potentiation of cell firing are also considered."}
{"id": "PMID:487185", "title": "Acute dendrotoxic changes in the hippocampus of kainate treated rats.", "content": "Kainic acid (KA), a potent neuroexcitatory and neurotoxic analog of glutamate (Glu), induces a widespread pattern of brain damage when administered subcutaneously to adult rats. The hippocampus is among the brain regions most consistently and severely damaged. Here we describe acute swelling of certain spines and branchlets of dendrites as the first detectable sign of KA neurotoxic changes in the hippocampus. These swellings conform to a laminar pattern suggesting selective toxic interaction of KA at specific levels of the dendritic trees of hippocampal pyramidal and dentate granule neurons. The frequency and severity of involvement of each type of hippocampal neuron at each level of its dendritic tree was roughly estimated and neuronal types were ranked from the most to least extensively involved (CA3 greater than CA4 greater than CA1 greater than CA2 greater than dentate granules). The same rank order has been described for the vulnerability of these neurons to acute destruction following intraventricular KA administration. Because the pattern of dendritic dilatations observed corresponds well with the pattern of termination of putative glutamergic inputs to the hippocampus, we interpret the findings as being consistent with the hypothesis that the toxic effects of KA are mediated through glutamergic excitatory receptors. We propose that the sensitivity of a given neuron to the neurodestructive action of KA may be determined by the percentage of its dendritic surface occupied by Glu receptors. We suspect that most, if not all, hippocampal neurons receive some glutamergic input and, therefore, are sensitive to KA. That CA3 pyramids are substantially more sensitive than dentate granules may signify that the former receive many more Glu terminals than the latter, an assumption quite consistent with our observation that focal dendritic swellings were both more densely and more widely distributed over the dendritic surfaces of the former than the latter.", "contents": "Acute dendrotoxic changes in the hippocampus of kainate treated rats. Kainic acid (KA), a potent neuroexcitatory and neurotoxic analog of glutamate (Glu), induces a widespread pattern of brain damage when administered subcutaneously to adult rats. The hippocampus is among the brain regions most consistently and severely damaged. Here we describe acute swelling of certain spines and branchlets of dendrites as the first detectable sign of KA neurotoxic changes in the hippocampus. These swellings conform to a laminar pattern suggesting selective toxic interaction of KA at specific levels of the dendritic trees of hippocampal pyramidal and dentate granule neurons. The frequency and severity of involvement of each type of hippocampal neuron at each level of its dendritic tree was roughly estimated and neuronal types were ranked from the most to least extensively involved (CA3 greater than CA4 greater than CA1 greater than CA2 greater than dentate granules). The same rank order has been described for the vulnerability of these neurons to acute destruction following intraventricular KA administration. Because the pattern of dendritic dilatations observed corresponds well with the pattern of termination of putative glutamergic inputs to the hippocampus, we interpret the findings as being consistent with the hypothesis that the toxic effects of KA are mediated through glutamergic excitatory receptors. We propose that the sensitivity of a given neuron to the neurodestructive action of KA may be determined by the percentage of its dendritic surface occupied by Glu receptors. We suspect that most, if not all, hippocampal neurons receive some glutamergic input and, therefore, are sensitive to KA. That CA3 pyramids are substantially more sensitive than dentate granules may signify that the former receive many more Glu terminals than the latter, an assumption quite consistent with our observation that focal dendritic swellings were both more densely and more widely distributed over the dendritic surfaces of the former than the latter."}
{"id": "PMID:487186", "title": "Quantitative studies of neuronal RNA on the subiculum of demented old individuals.", "content": "Autopsied human brains from three patients with senile dementia were studied for the effect of neurofibrillary tangles on neuronal RNA content. Nerve cell bodies were dissected out from the subiculum under the phase contrast microscope and were separated into two groups based on the presence or absence of neurofibrillary tangles within the perikaryon. It was found that the mean RNA content of the tangle-bearing cells was 28.61 pg, whereas that measured in nerve cell bodies considered as free from the tangles was 41.21 pg. Thus, a significant decrease in neuronal RNA content could be correlated to an excess accumulation of neurofibrillary tangles.", "contents": "Quantitative studies of neuronal RNA on the subiculum of demented old individuals. Autopsied human brains from three patients with senile dementia were studied for the effect of neurofibrillary tangles on neuronal RNA content. Nerve cell bodies were dissected out from the subiculum under the phase contrast microscope and were separated into two groups based on the presence or absence of neurofibrillary tangles within the perikaryon. It was found that the mean RNA content of the tangle-bearing cells was 28.61 pg, whereas that measured in nerve cell bodies considered as free from the tangles was 41.21 pg. Thus, a significant decrease in neuronal RNA content could be correlated to an excess accumulation of neurofibrillary tangles."}
{"id": "PMID:487187", "title": "Receptive fields of neurons in areas 17 and 18 of tree shrews (Tupaia glis).", "content": "Receptive fields of 245 cells in areas 17 and 18 were explored in tree shrews. Cells with simple and complex receptive fields were found in both areas but with different statistical distriubtions. A small percentage of non-oriented fields were found to be binocularly activated in both areas. Cells in area 17 preferred dark over light stimuli. Selective for direction, speed of stimulus movement, and level of background illumination, as well as combinations of these were common. Few hypercomplex cells were found. The superior retention of visually guided behavior of three shrews after lesions of the striate cortex is not explained by any radical difference between the properties of their striate neurons and those of other mammals.", "contents": "Receptive fields of neurons in areas 17 and 18 of tree shrews (Tupaia glis). Receptive fields of 245 cells in areas 17 and 18 were explored in tree shrews. Cells with simple and complex receptive fields were found in both areas but with different statistical distriubtions. A small percentage of non-oriented fields were found to be binocularly activated in both areas. Cells in area 17 preferred dark over light stimuli. Selective for direction, speed of stimulus movement, and level of background illumination, as well as combinations of these were common. Few hypercomplex cells were found. The superior retention of visually guided behavior of three shrews after lesions of the striate cortex is not explained by any radical difference between the properties of their striate neurons and those of other mammals."}
{"id": "PMID:487189", "title": "Removal of 14-C-palmitate during ventriculocisternal perfusion in conscious dogs in insulin-induced hypoglycemia.", "content": "A detailed description of a method is presented allowing continous ventriculocisternal perfusion of metabolites in the conscious dog. Using this preparation, the loss of the infused albuminbound 14-C-aplmitate, was studied in 14 conscious dogs during ventriculocisternal perfusion with artificial cerebrospinal fluid (CSF). Forty-five percent of the infused 14-C-palmitate was recovered from the cisternal effluent under equilibrium conditions after 60 min of perfusion. The effect of the injection of 2.0 U insulin/kg was also investigated in seven dogs. Plasma glucose concentration decreased to 40--50 mg% and the amount of 14-C-palmitate recovered was significantly higher one hour after insulin compared to the controls receiving saline injections. No significant changes in cerebral arteriovenous differences of glucose or oxygen or in venous 14-C-palmitate concentration were observed during the same time. It is concluded that the combined use of ventriculocisternal perfusion and the analysis of cerebral arteriovenous differences are useful in studying brain metabolism in the consciuos dog.", "contents": "Removal of 14-C-palmitate during ventriculocisternal perfusion in conscious dogs in insulin-induced hypoglycemia. A detailed description of a method is presented allowing continous ventriculocisternal perfusion of metabolites in the conscious dog. Using this preparation, the loss of the infused albuminbound 14-C-aplmitate, was studied in 14 conscious dogs during ventriculocisternal perfusion with artificial cerebrospinal fluid (CSF). Forty-five percent of the infused 14-C-palmitate was recovered from the cisternal effluent under equilibrium conditions after 60 min of perfusion. The effect of the injection of 2.0 U insulin/kg was also investigated in seven dogs. Plasma glucose concentration decreased to 40--50 mg% and the amount of 14-C-palmitate recovered was significantly higher one hour after insulin compared to the controls receiving saline injections. No significant changes in cerebral arteriovenous differences of glucose or oxygen or in venous 14-C-palmitate concentration were observed during the same time. It is concluded that the combined use of ventriculocisternal perfusion and the analysis of cerebral arteriovenous differences are useful in studying brain metabolism in the consciuos dog."}
{"id": "PMID:487190", "title": "The involvement of the olfactory bulbs in the regulation of gonadal and thyroidal activities of male red-winged blackbirds, exposed to short-day light regime.", "content": "Surgical removal of the olfactory bulbs (OB) was performed in mature male red-winged blackbirds, maintained under a short-day light regime. Bulbectomy caused hyperphagia, which was not accompanied by obesity. Bulbectomized (OBX) birds had incresaed thyroid follicular activity and had greater developed testes than sham-operated controls. In the adenohypophyses of the OB-removed birds there was an increase in the populations of 4 types of chromophils: alcianophils, PAS-positive basophils, orangeophils and PAS-positive acidophils. The possibility that the OB are involved in the photoperiodic regulation of the activity of the gonads and thyroids is discussed.", "contents": "The involvement of the olfactory bulbs in the regulation of gonadal and thyroidal activities of male red-winged blackbirds, exposed to short-day light regime. Surgical removal of the olfactory bulbs (OB) was performed in mature male red-winged blackbirds, maintained under a short-day light regime. Bulbectomy caused hyperphagia, which was not accompanied by obesity. Bulbectomized (OBX) birds had incresaed thyroid follicular activity and had greater developed testes than sham-operated controls. In the adenohypophyses of the OB-removed birds there was an increase in the populations of 4 types of chromophils: alcianophils, PAS-positive basophils, orangeophils and PAS-positive acidophils. The possibility that the OB are involved in the photoperiodic regulation of the activity of the gonads and thyroids is discussed."}
{"id": "PMID:487191", "title": "Effect of cycloheximide on temperature regulation in rats.", "content": "The effect of cycloheximide, an inhibitor of protein synthesis, on temperature regulation in afebrile rats was studied to determine whether its reported antipyretic effect might be attributable to a non-specific antimetabolic effect. Within 60 min after administration of cycloheximide (5 mg/kg IP) to female rats at ambient temperatures of 34, 25 and 15 degrees C, a significant decrease in colonic temperature was observed as compared with control rats administered saline IP. Measurements of rate of oxygen consumption showed that cycloheximide (5 mg/kg IP) significantly depressed the ability of rats to increase their heat production during a cold stress (15 degrees C). In the cold the rate of oxygen consumption increased 8.7 +/- 0.8 ml/min/kg0.75 in the control rats but only 1.8 +/- 0.9 ml/min/kg0.75 in the cycloheximide-treated rats. Since the thermoregulatory changes accompanying cold stress are similar to those observed during the genesis of fever, these data suggest that cycloheximide may have a general depressant effect on heat production rather than a specific antipyretic effect.", "contents": "Effect of cycloheximide on temperature regulation in rats. The effect of cycloheximide, an inhibitor of protein synthesis, on temperature regulation in afebrile rats was studied to determine whether its reported antipyretic effect might be attributable to a non-specific antimetabolic effect. Within 60 min after administration of cycloheximide (5 mg/kg IP) to female rats at ambient temperatures of 34, 25 and 15 degrees C, a significant decrease in colonic temperature was observed as compared with control rats administered saline IP. Measurements of rate of oxygen consumption showed that cycloheximide (5 mg/kg IP) significantly depressed the ability of rats to increase their heat production during a cold stress (15 degrees C). In the cold the rate of oxygen consumption increased 8.7 +/- 0.8 ml/min/kg0.75 in the control rats but only 1.8 +/- 0.9 ml/min/kg0.75 in the cycloheximide-treated rats. Since the thermoregulatory changes accompanying cold stress are similar to those observed during the genesis of fever, these data suggest that cycloheximide may have a general depressant effect on heat production rather than a specific antipyretic effect."}
{"id": "PMID:487192", "title": "Brainstem correlates of gustatory similarity in the hamster.", "content": "Responses of neurons in the nucleus tractus solitarius (NTS) and parabrachial pons (Pb pons) of the hamster to 10 gustatory stimuli were compared to behavioral similarities among these compounds. Animals were given a conditioned taste aversion to one of the 10 stimuli by pairing it with an induced gastrointestinal illness. Following this procedure, the degree of generalization of the learned taste aversion to each of the other compounds was measured. Behavioral similarity profiles were derived for each stimulus from the similarities in the generalization profiles of each pair of compounds. The across-neuron correlations in the firing rates evoked by these stimuli in the NTS cells corresponded quite well to these behavioral profiles, as did these neural correlations among Pb pontine cells, except for those correlations involving quinine. Sucrose-best cells in the Pb pons are too broadly responsive to account for the behavioral similarity functions for sweet-tasting stimuli, although other best-stimulus categories of cells (NaCl- and HCl-best) showed response profiles quite similar to the behavioral profiles, as did all best-stimulus classes of cells in the NTS.", "contents": "Brainstem correlates of gustatory similarity in the hamster. Responses of neurons in the nucleus tractus solitarius (NTS) and parabrachial pons (Pb pons) of the hamster to 10 gustatory stimuli were compared to behavioral similarities among these compounds. Animals were given a conditioned taste aversion to one of the 10 stimuli by pairing it with an induced gastrointestinal illness. Following this procedure, the degree of generalization of the learned taste aversion to each of the other compounds was measured. Behavioral similarity profiles were derived for each stimulus from the similarities in the generalization profiles of each pair of compounds. The across-neuron correlations in the firing rates evoked by these stimuli in the NTS cells corresponded quite well to these behavioral profiles, as did these neural correlations among Pb pontine cells, except for those correlations involving quinine. Sucrose-best cells in the Pb pons are too broadly responsive to account for the behavioral similarity functions for sweet-tasting stimuli, although other best-stimulus categories of cells (NaCl- and HCl-best) showed response profiles quite similar to the behavioral profiles, as did all best-stimulus classes of cells in the NTS."}
{"id": "PMID:487193", "title": "Septal destruction in infant rats and the ontogeny of drinking behaviors.", "content": "Male and female albino rat pups sustained septal destruction at 10 days of age and body weight and water intakes were measured daily throughout development until 200 days of age. During development the septal and control rats received a battery of drinking tests (e.g., cellular dehydration, hypovolemia, renin). Septal rats (males and females) were hyperdipsic for daily water intakes as early as 31 days of age and the daily hyperdipsia persisted through 200 days of age. On the battery of drinking tests, septal rats consumed water at control volumes on all tests except water deprivation (following which females were hyperdipsic) and food deprivation (during which both males and females were hyperdipsic). Urine output-water intake relationships were determined, and a series of food deprivation tests were conducted during development to determine the etiology of the developmental septal hyperdipsia. All results suggest that hyperdipsia associated with septal destruction during the preweaning period is a primary condition and not secondary to altered output mechanisms, abnormal feeding-drinking patterns, or displacement behavior.", "contents": "Septal destruction in infant rats and the ontogeny of drinking behaviors. Male and female albino rat pups sustained septal destruction at 10 days of age and body weight and water intakes were measured daily throughout development until 200 days of age. During development the septal and control rats received a battery of drinking tests (e.g., cellular dehydration, hypovolemia, renin). Septal rats (males and females) were hyperdipsic for daily water intakes as early as 31 days of age and the daily hyperdipsia persisted through 200 days of age. On the battery of drinking tests, septal rats consumed water at control volumes on all tests except water deprivation (following which females were hyperdipsic) and food deprivation (during which both males and females were hyperdipsic). Urine output-water intake relationships were determined, and a series of food deprivation tests were conducted during development to determine the etiology of the developmental septal hyperdipsia. All results suggest that hyperdipsia associated with septal destruction during the preweaning period is a primary condition and not secondary to altered output mechanisms, abnormal feeding-drinking patterns, or displacement behavior."}
{"id": "PMID:487194", "title": "A bipolar electrode for peripheral nerve stimulation.", "content": "The construction of a bipolar electrode for the stimulation of peripheral nerves is described. The electrode was designed for acute stimued in which the effects of peripheral nerve stimulation on central neural activity in rats were determined. Since the electrode can be secured into a fixed position, neural recordings can be made during the stimulation of various visceral nerves and the electrode can be easily adapted for chronic behavioral studies.", "contents": "A bipolar electrode for peripheral nerve stimulation. The construction of a bipolar electrode for the stimulation of peripheral nerves is described. The electrode was designed for acute stimued in which the effects of peripheral nerve stimulation on central neural activity in rats were determined. Since the electrode can be secured into a fixed position, neural recordings can be made during the stimulation of various visceral nerves and the electrode can be easily adapted for chronic behavioral studies."}
{"id": "PMID:487196", "title": "Morphological evidence that reline SMR and human mu are analogous rhythms.", "content": "In cats implanted with epidural screw electrodes over sensorimotor cortex, various recording montages were used to observe the waveform of the sensorimotor rhythm (SMR). It was found that in bipolar recordings spanning the crus, SMR exhibited a wicket-shaped waveform comparable in shape and polarity to the human mu rhythm. Based on previously reported topographic and functional similarities of feline SMR and human mu, and on the present data demonstrating morphological similarities, it appears that feline SMR is the first proven feline model of a spontaneous human ieeg rhythm.", "contents": "Morphological evidence that reline SMR and human mu are analogous rhythms. In cats implanted with epidural screw electrodes over sensorimotor cortex, various recording montages were used to observe the waveform of the sensorimotor rhythm (SMR). It was found that in bipolar recordings spanning the crus, SMR exhibited a wicket-shaped waveform comparable in shape and polarity to the human mu rhythm. Based on previously reported topographic and functional similarities of feline SMR and human mu, and on the present data demonstrating morphological similarities, it appears that feline SMR is the first proven feline model of a spontaneous human ieeg rhythm."}
{"id": "PMID:487197", "title": "Tapered tungsten fine-wire microelectrode for chronic single unit recording.", "content": "A new fine-wire microelectrode made from tungsten wire is described. The electrode design provides for strength as well as flexibility due to the stiffness of tungsten wire and a gradually tapering profile. Unlike previously-used fine wire electrodes, microelectrodes of the new design can be singly implanted under electrophysiological control into deep brain structures of larger animals such as cats. The flexibility of the fine diameter terminal portion of the microelectrode permits stable recording from caudal brain stem single units during vigorous behavioral activity.", "contents": "Tapered tungsten fine-wire microelectrode for chronic single unit recording. A new fine-wire microelectrode made from tungsten wire is described. The electrode design provides for strength as well as flexibility due to the stiffness of tungsten wire and a gradually tapering profile. Unlike previously-used fine wire electrodes, microelectrodes of the new design can be singly implanted under electrophysiological control into deep brain structures of larger animals such as cats. The flexibility of the fine diameter terminal portion of the microelectrode permits stable recording from caudal brain stem single units during vigorous behavioral activity."}
{"id": "PMID:487198", "title": "A minimum component AM transmitter to monitor electronic brain stimulation.", "content": "Construction of a light weight AM transmitter which can be used to monitor implanted electronic brain stimulators is described. The unit can operate continuously for 40 hours on a single battery and can easily be carried by a small laboratory animal. The range of the unit is limited to approximately 1 meter and the transmitted signal can be detected by a standard AM broadcast band receiver. The unit is simple to construct, inexpensive, and reliable in performance.", "contents": "A minimum component AM transmitter to monitor electronic brain stimulation. Construction of a light weight AM transmitter which can be used to monitor implanted electronic brain stimulators is described. The unit can operate continuously for 40 hours on a single battery and can easily be carried by a small laboratory animal. The range of the unit is limited to approximately 1 meter and the transmitted signal can be detected by a standard AM broadcast band receiver. The unit is simple to construct, inexpensive, and reliable in performance."}
{"id": "PMID:487199", "title": "Evoked potential correlates of stable inter-individual differences in rat behaviour.", "content": "Individual differences in rats' exploratory behaviour are stable over time, suggesting inherent differences in brain mechanisms for handling sensory information. These differences may have a neurophysiological basis since a negative correlation was found between individual variation in exploratory behaviour and recovery functions of cortical visual evoked potentials. Recovery functions were an expression of the extent to which the visual system recovered its responsiveness following initial stimulation. They were obtained by using pairs of flash stimuli separated by 3 interstimulus intervals (ISIs): 100, 200 and 300 msec. Recovery was defined as the amplitude of the response to the second flash expressed as a proportion of the response to the first flash. Only short latency components of the evoked responses were considered. The mean value for recovery averaged over the three ISIs constituted the recovery function. Only responses recorded when rats were quiet but alert were used for analysis. Evoked potential recovery functions appear to reflect a neurophysiological mechanism underlying the different capacities of individual rats for responding to novelty.", "contents": "Evoked potential correlates of stable inter-individual differences in rat behaviour. Individual differences in rats' exploratory behaviour are stable over time, suggesting inherent differences in brain mechanisms for handling sensory information. These differences may have a neurophysiological basis since a negative correlation was found between individual variation in exploratory behaviour and recovery functions of cortical visual evoked potentials. Recovery functions were an expression of the extent to which the visual system recovered its responsiveness following initial stimulation. They were obtained by using pairs of flash stimuli separated by 3 interstimulus intervals (ISIs): 100, 200 and 300 msec. Recovery was defined as the amplitude of the response to the second flash expressed as a proportion of the response to the first flash. Only short latency components of the evoked responses were considered. The mean value for recovery averaged over the three ISIs constituted the recovery function. Only responses recorded when rats were quiet but alert were used for analysis. Evoked potential recovery functions appear to reflect a neurophysiological mechanism underlying the different capacities of individual rats for responding to novelty."}
{"id": "PMID:487201", "title": "Effects of feedback caudate nucleus stimulation on spontaneous fast amygdaloid spindles and drive.", "content": "Experiments with single and repetitive stimulation of the head of the caudate nucleus (4-10 V, 0.3 msec) triggered by occurrence of fast amygdaloid spindles (35 c/s microV, 0.5 sec) were performed in six animals with chronically implanted cerebral electrodes. In conjunction with the anterior cortical recruiting response the stimulation increased the incidence of amygdaloid fast spindles and decreased the incidence of hippocampal theta activity and of searching eye movements. The associative training failed, however, to significantly modify in the background EEG, the mean incidence of the fast amygdaloid spindles or the rate of the sensorimotor rhythm of 12-18 c/s. Its only effect (after long repetition) was a displacement to the right of the regression line of the incidence of the two rhythms and a slight alteration in the slope. The possibility of activating the caudate nucleus inhibitory effects through the amygdala during internal inhibition phenomena is discussed.", "contents": "Effects of feedback caudate nucleus stimulation on spontaneous fast amygdaloid spindles and drive. Experiments with single and repetitive stimulation of the head of the caudate nucleus (4-10 V, 0.3 msec) triggered by occurrence of fast amygdaloid spindles (35 c/s microV, 0.5 sec) were performed in six animals with chronically implanted cerebral electrodes. In conjunction with the anterior cortical recruiting response the stimulation increased the incidence of amygdaloid fast spindles and decreased the incidence of hippocampal theta activity and of searching eye movements. The associative training failed, however, to significantly modify in the background EEG, the mean incidence of the fast amygdaloid spindles or the rate of the sensorimotor rhythm of 12-18 c/s. Its only effect (after long repetition) was a displacement to the right of the regression line of the incidence of the two rhythms and a slight alteration in the slope. The possibility of activating the caudate nucleus inhibitory effects through the amygdala during internal inhibition phenomena is discussed."}
{"id": "PMID:487203", "title": "Afferent projections to the self-stimulation regions of the dorsal pons, including the locus coeruleus, in the rat as demonstrated by the horseradish peroxidase technique.", "content": "Afferent projections to the dorsal pons of the rat have been studied using the horseradish peroxidase (HRP) technique. HRP injections were made in each of the following regions: the vicinity of the locus coeruleus (LC); the periventricular gray, medial to the LC; the medial parabrachial region, lateral to the LC; the ventral cerebellum, dorsal to the LC; and the pontine reticular formation, ventral to the LC. Because intracranial self-stimulation (ICSS) has been obtained in these regions, the afferents have been discussed in terms of their possible contributions to the behavior. Previous ICSS studies of the dorsal pons have focussed on the LC as playing a central role. Presently identified inputs to the LC include: the dorsal raphe nucleus: the ventrolateral periaqueductal gray: the pontine reticular formation: the areas that contain the pontine and medullary noradrenergic and adrenergic cell groups: the lateral hypothalamic area: the contralateral LC: the deep cerebellar nuclei: the ventrolateral and parafascicular thalamic nuclei: and the parabrachial regions of the pons and midbrain.", "contents": "Afferent projections to the self-stimulation regions of the dorsal pons, including the locus coeruleus, in the rat as demonstrated by the horseradish peroxidase technique. Afferent projections to the dorsal pons of the rat have been studied using the horseradish peroxidase (HRP) technique. HRP injections were made in each of the following regions: the vicinity of the locus coeruleus (LC); the periventricular gray, medial to the LC; the medial parabrachial region, lateral to the LC; the ventral cerebellum, dorsal to the LC; and the pontine reticular formation, ventral to the LC. Because intracranial self-stimulation (ICSS) has been obtained in these regions, the afferents have been discussed in terms of their possible contributions to the behavior. Previous ICSS studies of the dorsal pons have focussed on the LC as playing a central role. Presently identified inputs to the LC include: the dorsal raphe nucleus: the ventrolateral periaqueductal gray: the pontine reticular formation: the areas that contain the pontine and medullary noradrenergic and adrenergic cell groups: the lateral hypothalamic area: the contralateral LC: the deep cerebellar nuclei: the ventrolateral and parafascicular thalamic nuclei: and the parabrachial regions of the pons and midbrain."}
{"id": "PMID:487200", "title": "Histoenzymological mapping of alkaline phosphatase and 5-nucleotidase in the medulla oblongata of a microchiropteran bat (Taphozous melanopogon Temminck).", "content": "The contribution deals with the distribution of alkaline phosphatase and 5-nucleotidase in the medulla oblongata of Taphozous melanopogon (a Microchiroptera), for the first time. The main highlights of the study are: (1) Cranial nerve nuclei demonstrate intense activity of alkaline phosphatase, whereas except nucleus tractus spinalis n. trigemini, rest of the nuclei show much variations of 5-nucleotidase distribution. (2) The vestibular nuclei are very intensely positive for 5-nucleotidase but exhibit variable activity of alkaline phosphatase. (3) Three subdivisions of nucleus olivaris inferior, i.e. nucleus olivaris accessorius medialis, nucleus olivaris accessorius dorsalis and nucleus olivaris inferior, which were not reported in the earlier neuroanatomical studies, have been identified. These nuclei show very intense alkaline phosphatase and relatively less intense 5-nucleotidase activities. (4) In general, the activity of alkaline phosphatase is stronger in neurons than in neuropil and that of 5-nucleotidase is stronger in neuropil than in neurons. (5) Blood capillaries are completely negative for alkaline phosphatase and intensely positive for 5-nucleotidase. A comparison is made between the distribution of these enzymes in the medulla oblongata of bat and other mammals so far studied.", "contents": "Histoenzymological mapping of alkaline phosphatase and 5-nucleotidase in the medulla oblongata of a microchiropteran bat (Taphozous melanopogon Temminck). The contribution deals with the distribution of alkaline phosphatase and 5-nucleotidase in the medulla oblongata of Taphozous melanopogon (a Microchiroptera), for the first time. The main highlights of the study are: (1) Cranial nerve nuclei demonstrate intense activity of alkaline phosphatase, whereas except nucleus tractus spinalis n. trigemini, rest of the nuclei show much variations of 5-nucleotidase distribution. (2) The vestibular nuclei are very intensely positive for 5-nucleotidase but exhibit variable activity of alkaline phosphatase. (3) Three subdivisions of nucleus olivaris inferior, i.e. nucleus olivaris accessorius medialis, nucleus olivaris accessorius dorsalis and nucleus olivaris inferior, which were not reported in the earlier neuroanatomical studies, have been identified. These nuclei show very intense alkaline phosphatase and relatively less intense 5-nucleotidase activities. (4) In general, the activity of alkaline phosphatase is stronger in neurons than in neuropil and that of 5-nucleotidase is stronger in neuropil than in neurons. (5) Blood capillaries are completely negative for alkaline phosphatase and intensely positive for 5-nucleotidase. A comparison is made between the distribution of these enzymes in the medulla oblongata of bat and other mammals so far studied."}
{"id": "PMID:487204", "title": "The effect of medial preoptic--anterior hypothalamic lesions on bisexual behavior of the male rat.", "content": "The effects of bilateral radiofrequency lesions in various parts of the medial preoptic area (mPOA) and anterior hypothalamus (AH) on masculine and hormone induced feminine sexual behavior was studied in male rats. Anatomical as well as statistical analysis of the extent and location of the lesions and the consequent behavioral changes indicated that different parts of the mPOA-AH continuum are involved in masculine and feminine sexual behavior in different ways. Bilateral destruction at the transition of the mPOA and AH resulted in a large reduction of masculine sexual activity while lesions concentrated in the AH slightly facilitated feminine sexual behavior in males. These results are consistent with the notion that the mPOA-AH continuum is divided into anatomically and functionally different parts.", "contents": "The effect of medial preoptic--anterior hypothalamic lesions on bisexual behavior of the male rat. The effects of bilateral radiofrequency lesions in various parts of the medial preoptic area (mPOA) and anterior hypothalamus (AH) on masculine and hormone induced feminine sexual behavior was studied in male rats. Anatomical as well as statistical analysis of the extent and location of the lesions and the consequent behavioral changes indicated that different parts of the mPOA-AH continuum are involved in masculine and feminine sexual behavior in different ways. Bilateral destruction at the transition of the mPOA and AH resulted in a large reduction of masculine sexual activity while lesions concentrated in the AH slightly facilitated feminine sexual behavior in males. These results are consistent with the notion that the mPOA-AH continuum is divided into anatomically and functionally different parts."}
{"id": "PMID:487205", "title": "Lesions of the preoptic area facilitate lordosis behavior in male and female guinea pigs.", "content": "Male and female guinea pigs received radiofrequency lesions in the medial preoptic area (MPOA). Animals were gonadectomized, treated with estrogen and progesterone, and tested for the occurrence of the lordosis response to manual stimulation. Females with MPOA lesions exhibited enhanced lordosis behavior, shorter latencies to heat, longer duration of heat and longer maximum lordosis duration than sham control females. In males with MPOA lesions, the lordosis response could be elicited by manual stimulation, in contrast to no response in the sham control males. Furthermore, MPOA-lesioned males were insensitive to the inhibitory effects of progesterone on lordosis behavior, while MPOA-lesioned females were as sensitive as sham controls to the inhibitory effects of progesterone. The results suggest that a neural mechanism resides within the MPOA which inhibits the occurrence of lordosis behavior in both male and female guinea pigs and which is not involved in a sexual dimorphism in responsiveness to progesterone.", "contents": "Lesions of the preoptic area facilitate lordosis behavior in male and female guinea pigs. Male and female guinea pigs received radiofrequency lesions in the medial preoptic area (MPOA). Animals were gonadectomized, treated with estrogen and progesterone, and tested for the occurrence of the lordosis response to manual stimulation. Females with MPOA lesions exhibited enhanced lordosis behavior, shorter latencies to heat, longer duration of heat and longer maximum lordosis duration than sham control females. In males with MPOA lesions, the lordosis response could be elicited by manual stimulation, in contrast to no response in the sham control males. Furthermore, MPOA-lesioned males were insensitive to the inhibitory effects of progesterone on lordosis behavior, while MPOA-lesioned females were as sensitive as sham controls to the inhibitory effects of progesterone. The results suggest that a neural mechanism resides within the MPOA which inhibits the occurrence of lordosis behavior in both male and female guinea pigs and which is not involved in a sexual dimorphism in responsiveness to progesterone."}
{"id": "PMID:487206", "title": "Fastigial modulation of brainstem behavioral mechanisms.", "content": "Stimulation of the rostral, but not caudal, half of the cerebellar fastigial nucleus was found to induce coordinated eating and grooming behaviors in the cat. These behaviors persisted, unchanged, after lesions of the ascending fastigial projections in the superior cerebellar peduncle, suggesting that such behaviors are produced by direct fastigio-bulbar pathways. These results support the suggestion that there is a close anatomical association between fastigial systems mediating elicited behaviors and those mediating fastigial pressor responses. The present results are also consistent with the growing view that cerebellar systems can exert important modulatory influences on neurobehavioral mechanisms.", "contents": "Fastigial modulation of brainstem behavioral mechanisms. Stimulation of the rostral, but not caudal, half of the cerebellar fastigial nucleus was found to induce coordinated eating and grooming behaviors in the cat. These behaviors persisted, unchanged, after lesions of the ascending fastigial projections in the superior cerebellar peduncle, suggesting that such behaviors are produced by direct fastigio-bulbar pathways. These results support the suggestion that there is a close anatomical association between fastigial systems mediating elicited behaviors and those mediating fastigial pressor responses. The present results are also consistent with the growing view that cerebellar systems can exert important modulatory influences on neurobehavioral mechanisms."}
{"id": "PMID:487208", "title": "Influence of electrical stimulation of the substantia nigra on spontaneous activity of raphe neurons in the anesthetized rat.", "content": "Recent studies of afferent connections of the anterior raphe using the horseradish peroxidase technique have demonstrated a major projection originating in the substantia nigra (SN). The present acute electrophysiological study examined the influence of stimulation of the afferent on the activity of individual neurons in the raphe of the posterior midbrain and anterior pons (n = 51), and of a control group of cells (n = 15) located 2 mm lateral to the raphe. The predominant effect of SN stimulation at 0.1-1.0 mA, 1 Hz or 10 Hz, was suppression of raphe activity, with 63% of the cells showing cessation of firing following SN pulses and only 8% showing excitation. The average duration of suppression was 200 msec at 1 Hz and 38 msec at 10 Hz. In contrast, 40% of the lateral cells were excited, with 27% of the cells showing suppression. The mean duration of total suppression of lateral cell firing was 61 and 17 msec at 1 and 10 Hz, respectively. The results from the raphe cells are consistent with recent reports of stimulation of other forebrain and brainstem afferents to the raphe in which suppression of raphe activity was the main effect.", "contents": "Influence of electrical stimulation of the substantia nigra on spontaneous activity of raphe neurons in the anesthetized rat. Recent studies of afferent connections of the anterior raphe using the horseradish peroxidase technique have demonstrated a major projection originating in the substantia nigra (SN). The present acute electrophysiological study examined the influence of stimulation of the afferent on the activity of individual neurons in the raphe of the posterior midbrain and anterior pons (n = 51), and of a control group of cells (n = 15) located 2 mm lateral to the raphe. The predominant effect of SN stimulation at 0.1-1.0 mA, 1 Hz or 10 Hz, was suppression of raphe activity, with 63% of the cells showing cessation of firing following SN pulses and only 8% showing excitation. The average duration of suppression was 200 msec at 1 Hz and 38 msec at 10 Hz. In contrast, 40% of the lateral cells were excited, with 27% of the cells showing suppression. The mean duration of total suppression of lateral cell firing was 61 and 17 msec at 1 and 10 Hz, respectively. The results from the raphe cells are consistent with recent reports of stimulation of other forebrain and brainstem afferents to the raphe in which suppression of raphe activity was the main effect."}
{"id": "PMID:487202", "title": "Mapping of sensory-responsive cells in the septal area of the rat.", "content": "A study of sensory-responsive characteristics of single cells of the septal area of male rats was undertaken to determine the distribution of stimulation-induced changes in septal activity. Single cells were systematically sampled in six planes transecting the rostral-caudal axis of the septal area and were tested for responsiveness to visual, auditory, tactual, gustatory and olfactory stimuli. Approximately 50% of the sampled population of septal cells was responsive to at least one stimulus. Most of the responses of septal cells to stimulation were excitatory and occurred at the onset of stimulation. Regions of concentration of sensory-responsiveness cells in the system were found to lie in medial areas of the septum consisting of the dorsomedial portion of the lateral septal nucleus, the medial septal nucleus and the ventral portions of the medial and lateral nuclei. Visual, auditory and tactual stimuli were most effective in producing changes in spontaneous activity of units in these regions. On the basis of relationships of the medial septal region to other limbic areas having sensory-responsive properties, it is concluded that the medial septal area is one component of a limbic sensory system.", "contents": "Mapping of sensory-responsive cells in the septal area of the rat. A study of sensory-responsive characteristics of single cells of the septal area of male rats was undertaken to determine the distribution of stimulation-induced changes in septal activity. Single cells were systematically sampled in six planes transecting the rostral-caudal axis of the septal area and were tested for responsiveness to visual, auditory, tactual, gustatory and olfactory stimuli. Approximately 50% of the sampled population of septal cells was responsive to at least one stimulus. Most of the responses of septal cells to stimulation were excitatory and occurred at the onset of stimulation. Regions of concentration of sensory-responsiveness cells in the system were found to lie in medial areas of the septum consisting of the dorsomedial portion of the lateral septal nucleus, the medial septal nucleus and the ventral portions of the medial and lateral nuclei. Visual, auditory and tactual stimuli were most effective in producing changes in spontaneous activity of units in these regions. On the basis of relationships of the medial septal region to other limbic areas having sensory-responsive properties, it is concluded that the medial septal area is one component of a limbic sensory system."}
{"id": "PMID:487211", "title": "A rapid and simple cannulation technique for repeated sampling of cerebrospinal fluid in freely moving rats.", "content": "A cannulation technique for frequent sampling of cerebrospinal fluid (CSF) in unanaesthetized freely moving rats is described. A permanent stainless steel cannula, constructed in such a way that no loss of CSF occurs, is placed into the rat's cisterna magna and fixed to the skull by anchoring screws and dental cement. A special CSF outflow opening of the cannula is connected to polyethylene tubing for CSF sampling. Amounts of 50-150 microliters CSF can be collected repeatedly without any sign of disturbing the animal. The technique lends itself not only to pilot studies in which within a short period of time a large amount of CSF is wanted, but also to experiments in which physiological conditions are required.", "contents": "A rapid and simple cannulation technique for repeated sampling of cerebrospinal fluid in freely moving rats. A cannulation technique for frequent sampling of cerebrospinal fluid (CSF) in unanaesthetized freely moving rats is described. A permanent stainless steel cannula, constructed in such a way that no loss of CSF occurs, is placed into the rat's cisterna magna and fixed to the skull by anchoring screws and dental cement. A special CSF outflow opening of the cannula is connected to polyethylene tubing for CSF sampling. Amounts of 50-150 microliters CSF can be collected repeatedly without any sign of disturbing the animal. The technique lends itself not only to pilot studies in which within a short period of time a large amount of CSF is wanted, but also to experiments in which physiological conditions are required."}
{"id": "PMID:487207", "title": "Differential hypothalamic self-stimulation behaviour in Roman high-avoidance and low-avoidance rats.", "content": "Monopolar electrodes were implanted bilaterally and symmetrically into a small well defined region of the posterior lateral hypothalamus in 11 rats of the selected strains Roman high-avoidance (RHA/Verh, 6 animals) and Roman low-avoidance (RLA/Verh, 5 animals). Intracranial electrical stimulation (ICS) was used to study possible strain difference with respect to self-stimulation behaviour. Using a shuttle-box, the experimental set-up allowed measurements of the animal's preference, aversion, neutrality or ambivalence towards the stimulus. For both rat strains 10 electrode sites were tested with identical stimulation currents (100-600 microA in increments of 100 microA). Significant differences between the selected strains were found for preference (self-stimulation) and aversion: RLA/Verh-rats showed self-stimulation at lower stimulation currents than RHA/Verh-rats. At higher currents, both strains showed similar self-stimulation performance. RLA/Verh-rats escaped more often from ICS yielding self-stimulation at five of the six current levels. It is concluded that, in comparison with the RHA strain, RLA/Verh-rats are more sensitive to aversive effect of lateral hypothalamic stimulation. This seems to be associated with an increased sensitivity to the rewarding effects of such stimulation.", "contents": "Differential hypothalamic self-stimulation behaviour in Roman high-avoidance and low-avoidance rats. Monopolar electrodes were implanted bilaterally and symmetrically into a small well defined region of the posterior lateral hypothalamus in 11 rats of the selected strains Roman high-avoidance (RHA/Verh, 6 animals) and Roman low-avoidance (RLA/Verh, 5 animals). Intracranial electrical stimulation (ICS) was used to study possible strain difference with respect to self-stimulation behaviour. Using a shuttle-box, the experimental set-up allowed measurements of the animal's preference, aversion, neutrality or ambivalence towards the stimulus. For both rat strains 10 electrode sites were tested with identical stimulation currents (100-600 microA in increments of 100 microA). Significant differences between the selected strains were found for preference (self-stimulation) and aversion: RLA/Verh-rats showed self-stimulation at lower stimulation currents than RHA/Verh-rats. At higher currents, both strains showed similar self-stimulation performance. RLA/Verh-rats escaped more often from ICS yielding self-stimulation at five of the six current levels. It is concluded that, in comparison with the RHA strain, RLA/Verh-rats are more sensitive to aversive effect of lateral hypothalamic stimulation. This seems to be associated with an increased sensitivity to the rewarding effects of such stimulation."}
{"id": "PMID:487212", "title": "Latency distribution from orthodromic stimulation at the optic nerve, in the lateral geniculate nucleus and superior colliculus of rabbits.", "content": "Latency distribution of responses to optic nerve stimulation has been compared between the Lateral Geniculate Nucleus (L.G.N.) and Superior Colliculus (S.C.), in rabbits. Histograms revealed that in both structures the latency distributions are very similar. Results suggest that all groups of retinofugal fibers project to both sites, although longer latencies are more frequently met at the collicular level. In addition evidence is presented that in rabbit's optic nerve there are at least three populations of axons with different conduction velocities.", "contents": "Latency distribution from orthodromic stimulation at the optic nerve, in the lateral geniculate nucleus and superior colliculus of rabbits. Latency distribution of responses to optic nerve stimulation has been compared between the Lateral Geniculate Nucleus (L.G.N.) and Superior Colliculus (S.C.), in rabbits. Histograms revealed that in both structures the latency distributions are very similar. Results suggest that all groups of retinofugal fibers project to both sites, although longer latencies are more frequently met at the collicular level. In addition evidence is presented that in rabbit's optic nerve there are at least three populations of axons with different conduction velocities."}
{"id": "PMID:487210", "title": "Effects of ovarian hormones on levels of luteinizing hormone in plasma and on serotonin concentrations in discrete brain nuclei.", "content": "Levels of serotonin were measureed in microdissected, individual brain nuclei in ovariectomized rats after treatment with ovarian hormones. Regions sampled included nuclei in the forebrain, rostral and medial hypothalamus, and midbrain tegmentum. Estradiol benzoate decreased levels of luteinizing hormone in plasma but did not affect serotonin levels in any region. Progesterone alone elevated serotonin content in the nucleus tractus diagonalis and ventral tegmental area. The combined estrogen plus progesterone regime produced a surge in plasma luteinizing hormone and also markedly elevated serotonin in the median eminence. These results may be of significance for ovarian hormonal regulation of gonadotropin secretion and reproductive behavior.", "contents": "Effects of ovarian hormones on levels of luteinizing hormone in plasma and on serotonin concentrations in discrete brain nuclei. Levels of serotonin were measureed in microdissected, individual brain nuclei in ovariectomized rats after treatment with ovarian hormones. Regions sampled included nuclei in the forebrain, rostral and medial hypothalamus, and midbrain tegmentum. Estradiol benzoate decreased levels of luteinizing hormone in plasma but did not affect serotonin levels in any region. Progesterone alone elevated serotonin content in the nucleus tractus diagonalis and ventral tegmental area. The combined estrogen plus progesterone regime produced a surge in plasma luteinizing hormone and also markedly elevated serotonin in the median eminence. These results may be of significance for ovarian hormonal regulation of gonadotropin secretion and reproductive behavior."}
{"id": "PMID:487213", "title": "Elevation of calcitonin immunoreactivity in the pituitary and thyroid glands of genetically obese rats (fa/fa).", "content": "This paper contains the first demonstration of quantitative changes in the levels of calcitonin-like immunoreactivity in the pituitary. The concentration of calcitonin-like material in lean Zucker rats (?/+) was 0.42 +/- 0.09 ng/mg wet weight of pituitary. The pituitaries of obese rats (fa/fa) contained significantly greater levels (1.56 +/- 0.56 ng/mg wet weight). This 271% increase represents the first indication that pituitary calcitonin-like material may have a physiological role in genetically obese rats. Thyroidal calcitonin also elevated in the obese (111%). These large elevations in the calcitonin content of the glands of obese rats were not accompanied by significant elevations of calcitonin in the blood. This suggests that obese rats have problems with mechanisms for the release of calcitonin into the blood and might not be able to combat hypercalcemia as effectively as leans. However, this hypothesis remains to be tested.", "contents": "Elevation of calcitonin immunoreactivity in the pituitary and thyroid glands of genetically obese rats (fa/fa). This paper contains the first demonstration of quantitative changes in the levels of calcitonin-like immunoreactivity in the pituitary. The concentration of calcitonin-like material in lean Zucker rats (?/+) was 0.42 +/- 0.09 ng/mg wet weight of pituitary. The pituitaries of obese rats (fa/fa) contained significantly greater levels (1.56 +/- 0.56 ng/mg wet weight). This 271% increase represents the first indication that pituitary calcitonin-like material may have a physiological role in genetically obese rats. Thyroidal calcitonin also elevated in the obese (111%). These large elevations in the calcitonin content of the glands of obese rats were not accompanied by significant elevations of calcitonin in the blood. This suggests that obese rats have problems with mechanisms for the release of calcitonin into the blood and might not be able to combat hypercalcemia as effectively as leans. However, this hypothesis remains to be tested."}
{"id": "PMID:487209", "title": "Determination of picogram levels of brain serotonin by a simplified liquid chromatographic electrochemical detection assay.", "content": "A simplified assay is described for measurement of picogram amounts of serotonin (5-HT) by high pressure liquid chromatography with electrochemical detection (LCEC). The procedure involves pre-purification of brain 5-HT by adsorption on Amberlite CG-50. Serotonin is subsequently resolved and detected by LCEC on Zipax SCX resin. The present method gives working sensitivities of at least 100 pg, tissue recoveries of 95% and very low interassay variability (coefficient of variation = 3%). Determination of rat brain area 5-HT by this LCEC method is described and compared to other high sensitivity methods.", "contents": "Determination of picogram levels of brain serotonin by a simplified liquid chromatographic electrochemical detection assay. A simplified assay is described for measurement of picogram amounts of serotonin (5-HT) by high pressure liquid chromatography with electrochemical detection (LCEC). The procedure involves pre-purification of brain 5-HT by adsorption on Amberlite CG-50. Serotonin is subsequently resolved and detected by LCEC on Zipax SCX resin. The present method gives working sensitivities of at least 100 pg, tissue recoveries of 95% and very low interassay variability (coefficient of variation = 3%). Determination of rat brain area 5-HT by this LCEC method is described and compared to other high sensitivity methods."}
{"id": "PMID:487214", "title": "Distribution of norepinephrine and dopamine in cerebral cortical areas of the rat.", "content": "Concentrations of norepinephrine and dopamine were determined using enzyme isotope assay in 27 microdissected cerebral cortical areas of the rat. A detailed map is presented for microdissection of rat cerebral cortex. Norepinephrine was found in low but still measurable quantities throughout the cortex. Differences between cortical areas are also low. Relatively highest levels were demonstrated in the pyriform, insular and entorhinal cortices. The distribution of dopamine was found to be uneven with a maximal regional difference of 1:24. Concentration of dopamine was in all areas lower than that of norepinephrine. The highest dopamine concentration (2,4 ng/mg protein) was measured in the rostral pyriform cortex but other mesocortical (cingulate, frontal, insular and entorhinal) dopaminergic areas also contained relatively high amounts. Except for the caudal occipital and caudal entorhinal cortices all regions studied contained measurable quantities of dopamine. Its low concentration relative to norepinephrine (below 15%) suggests that in the cortical areas studied dopamine is present as the precursor of norepinephrine.", "contents": "Distribution of norepinephrine and dopamine in cerebral cortical areas of the rat. Concentrations of norepinephrine and dopamine were determined using enzyme isotope assay in 27 microdissected cerebral cortical areas of the rat. A detailed map is presented for microdissection of rat cerebral cortex. Norepinephrine was found in low but still measurable quantities throughout the cortex. Differences between cortical areas are also low. Relatively highest levels were demonstrated in the pyriform, insular and entorhinal cortices. The distribution of dopamine was found to be uneven with a maximal regional difference of 1:24. Concentration of dopamine was in all areas lower than that of norepinephrine. The highest dopamine concentration (2,4 ng/mg protein) was measured in the rostral pyriform cortex but other mesocortical (cingulate, frontal, insular and entorhinal) dopaminergic areas also contained relatively high amounts. Except for the caudal occipital and caudal entorhinal cortices all regions studied contained measurable quantities of dopamine. Its low concentration relative to norepinephrine (below 15%) suggests that in the cortical areas studied dopamine is present as the precursor of norepinephrine."}
{"id": "PMID:487215", "title": "Taurine, analogues and ethanol elicited responses.", "content": "The effect of taurine, of some of its precursors and major metabolic products on spontaneous locomotor activity were studied in mice. The effect of taurine and some analogues on certain ethanol-mediated responses were observed. Administration of taurine, 50 mg/kg, IP, did not significantly alter motility in experimental animals compared to controls. Behavioral depression was evident subsequent to injection of cysteine hydrochloride or taurocholic acid (50 mg/kg). Administration of taurocholic acid, 50 mg/kg, IP, 30 min prior to a narcotic dose of ethanol, 5 g/kg, IP, reduced the time required for the onset of ethanol-narcosis. Pretreatment with cysteic acid, 50 mg/kg, IP, prolonged ethanol-produced narcosis. Treatment with cysteic acid 30 min prior to ethanol, 2.5 g/kg, IP, was found to decrease whole blood ethanol concentration as compared to the respective controls without a concomitant changes in brain ethanol levels. Administration of taurocholic acid, 100 mg/kg, IP, decreased the intake of an ethanol solution in rats preferring 5% ethanol solution over water as the drinking fluid of choice. None of the compounds tested altered endogenous specific activity of mouse liver alcohol dehydrogenase when given once daily (50 mg/kg, IP) for 10 consecutive days. The results suggest that both taurocholic acid and cysteic acid exert additive action to some ethanol-elicited responses studied.", "contents": "Taurine, analogues and ethanol elicited responses. The effect of taurine, of some of its precursors and major metabolic products on spontaneous locomotor activity were studied in mice. The effect of taurine and some analogues on certain ethanol-mediated responses were observed. Administration of taurine, 50 mg/kg, IP, did not significantly alter motility in experimental animals compared to controls. Behavioral depression was evident subsequent to injection of cysteine hydrochloride or taurocholic acid (50 mg/kg). Administration of taurocholic acid, 50 mg/kg, IP, 30 min prior to a narcotic dose of ethanol, 5 g/kg, IP, reduced the time required for the onset of ethanol-narcosis. Pretreatment with cysteic acid, 50 mg/kg, IP, prolonged ethanol-produced narcosis. Treatment with cysteic acid 30 min prior to ethanol, 2.5 g/kg, IP, was found to decrease whole blood ethanol concentration as compared to the respective controls without a concomitant changes in brain ethanol levels. Administration of taurocholic acid, 100 mg/kg, IP, decreased the intake of an ethanol solution in rats preferring 5% ethanol solution over water as the drinking fluid of choice. None of the compounds tested altered endogenous specific activity of mouse liver alcohol dehydrogenase when given once daily (50 mg/kg, IP) for 10 consecutive days. The results suggest that both taurocholic acid and cysteic acid exert additive action to some ethanol-elicited responses studied."}
{"id": "PMID:487216", "title": "Effects of morphine on hippocampal cells recorded in vitro.", "content": "Intracellular recordings from CA1 pyramidal cells were obtained in the in vitro hippocampal slice preparation before and after the application of morphine sulfate. Two distinct effects were observed following addition of morphine (1.0 mM) to the bathing media, or application to individual slices through pressure ejection via large tipped pipettes. Within 3-10 min following morphine application CA1 cell membrane potentials began to depolarize (5-10 mV) toward firing threshold. Spontaneous discharge rates were increased and nonactive cells became active. Rhythmic firing at a rate of 5-8 Hz was observed for 30-40 min following drug application. The spontaneous rhythmic firing could be partially reversed by the addition of naloxone (0.1 mM) to the bathing media. The second effect of morphine was to potentiate the development of epileptiform discharges in CA1 cells in response to low frequency orthodromic synaptic stimulation. The findings indicate that morphine alters the excitability of hippocampal pyramidal cells and such changes are likely to be mediated by opiate specific alterations in membrane conductance and/or tonic inhibitory synaptic inputs.", "contents": "Effects of morphine on hippocampal cells recorded in vitro. Intracellular recordings from CA1 pyramidal cells were obtained in the in vitro hippocampal slice preparation before and after the application of morphine sulfate. Two distinct effects were observed following addition of morphine (1.0 mM) to the bathing media, or application to individual slices through pressure ejection via large tipped pipettes. Within 3-10 min following morphine application CA1 cell membrane potentials began to depolarize (5-10 mV) toward firing threshold. Spontaneous discharge rates were increased and nonactive cells became active. Rhythmic firing at a rate of 5-8 Hz was observed for 30-40 min following drug application. The spontaneous rhythmic firing could be partially reversed by the addition of naloxone (0.1 mM) to the bathing media. The second effect of morphine was to potentiate the development of epileptiform discharges in CA1 cells in response to low frequency orthodromic synaptic stimulation. The findings indicate that morphine alters the excitability of hippocampal pyramidal cells and such changes are likely to be mediated by opiate specific alterations in membrane conductance and/or tonic inhibitory synaptic inputs."}
{"id": "PMID:487217", "title": "Morphine induced increases in the incorporation of 3H-thymidine into brain striatal DNA.", "content": "3H-thymidine uptake into DNA fractions of rat brain regions was measured following in vivo administration of (methyl-3H)-thymidine and morphine. Acute morphine administration (10 mg/kg; 30 min prior to 3H-thymidine) increased incorporation of 3H-thymidine into DNA of rat striatum. This effect was antagonized by naloxone (1 mg/kg). Further, the observed change in incorporation of 3H-thymidine into DNA in striatum could not be accounted for by differences in the local availability of the label in morphinized rats. An autoradiographic study revealed that the 3H-thymidine was localized in nuclei in cells of the sub-ependymal layer lining the lateral ventricles, an area of glial cell proliferation in adult rats. No change in 3H-thymidine incorporation into DNA was observed in any area of the brain in morphine-addicted rats or in rats undergoing naloxone-precipitated withdrawal. The results indicate that opiates may induce permanent anatomical changes in the brain, including alterations of neuroglial interactions.", "contents": "Morphine induced increases in the incorporation of 3H-thymidine into brain striatal DNA. 3H-thymidine uptake into DNA fractions of rat brain regions was measured following in vivo administration of (methyl-3H)-thymidine and morphine. Acute morphine administration (10 mg/kg; 30 min prior to 3H-thymidine) increased incorporation of 3H-thymidine into DNA of rat striatum. This effect was antagonized by naloxone (1 mg/kg). Further, the observed change in incorporation of 3H-thymidine into DNA in striatum could not be accounted for by differences in the local availability of the label in morphinized rats. An autoradiographic study revealed that the 3H-thymidine was localized in nuclei in cells of the sub-ependymal layer lining the lateral ventricles, an area of glial cell proliferation in adult rats. No change in 3H-thymidine incorporation into DNA was observed in any area of the brain in morphine-addicted rats or in rats undergoing naloxone-precipitated withdrawal. The results indicate that opiates may induce permanent anatomical changes in the brain, including alterations of neuroglial interactions."}
{"id": "PMID:487218", "title": "Cerebellar actions on cochlear microphonics and on auditory nerve action potential.", "content": "The influence exerted by cerebellar stimulation upon cochlear microphonics (CM) and auditory nerve action potential (AP) has been analyzed in curarized guinea pigs. Round window recordings demonstrated that conditioning electrical stimulus trains delivered to the cerebellar cortex diminished the CM and AP amplitude at the same time and in a parallel fashion. On the other hand, cooling of the cerebellar cortex showed the opposite results of increased amplitudes. All pre-receptorial mechanisms were avoided. A PDP-12 computer performed parametric and non-parametric statistical analysis showing the differences to be significant for the shifts. Evidence of simultaneous inhibitory cerebellar action on both potentials has been demonstrated and a cerebello-olivo-cochlear pathway is proposed for such action upon the receptor cells and/or incoming fibers.", "contents": "Cerebellar actions on cochlear microphonics and on auditory nerve action potential. The influence exerted by cerebellar stimulation upon cochlear microphonics (CM) and auditory nerve action potential (AP) has been analyzed in curarized guinea pigs. Round window recordings demonstrated that conditioning electrical stimulus trains delivered to the cerebellar cortex diminished the CM and AP amplitude at the same time and in a parallel fashion. On the other hand, cooling of the cerebellar cortex showed the opposite results of increased amplitudes. All pre-receptorial mechanisms were avoided. A PDP-12 computer performed parametric and non-parametric statistical analysis showing the differences to be significant for the shifts. Evidence of simultaneous inhibitory cerebellar action on both potentials has been demonstrated and a cerebello-olivo-cochlear pathway is proposed for such action upon the receptor cells and/or incoming fibers."}
{"id": "PMID:487219", "title": "Hypothermia produced by peripheral and central injections of chlorpromazine in aged rabbits.", "content": "Hypothermia produced by IV administration of chlorpromazine (CPZ, 0.5-2.0 mg/kg) in a thermoneutral environment was greater in rabbits 2-4.5 years old than in animals under 24 months of age. One microgram CPZ given intracerebroventricularly (ICV) produced greater hypothermia in the older animals in tests performed in a thermoneutral environment while 0.25 and 0.5 microgram doses did not. The hypothermogenic effect of all three ICV doses of CPZ was enhanced in older rabbits exposed to cold. The brain of the older rabbit appears to be more sensitive to the hypothermogenic effects of CPZ. The findings suggest that this widely used tranquilizer can contribute to accidental hypothermia of the aged.", "contents": "Hypothermia produced by peripheral and central injections of chlorpromazine in aged rabbits. Hypothermia produced by IV administration of chlorpromazine (CPZ, 0.5-2.0 mg/kg) in a thermoneutral environment was greater in rabbits 2-4.5 years old than in animals under 24 months of age. One microgram CPZ given intracerebroventricularly (ICV) produced greater hypothermia in the older animals in tests performed in a thermoneutral environment while 0.25 and 0.5 microgram doses did not. The hypothermogenic effect of all three ICV doses of CPZ was enhanced in older rabbits exposed to cold. The brain of the older rabbit appears to be more sensitive to the hypothermogenic effects of CPZ. The findings suggest that this widely used tranquilizer can contribute to accidental hypothermia of the aged."}
{"id": "PMID:487220", "title": "Intracellularly studied excitability changes in coronal-pericruciate neurons following low frequency stimulation of the corticobulbar tract.", "content": "Direct intracellular measurements of excitability changes to injected current were made in 116 cortical neurons of awake cats following antidromic or orthodromic activation by repetitive, 1-6 Hz stimulation of the pes pedunculi. The predominant effect seen in antidromically activated cells was a transient decrease in excitability and input resistance. The predominant effect seen in orthodromically (transsynaptically) activated cells was a transient increase in excitability and input resistance. In the absence of accompanying change in rates of spontaneous spike activity, the above results suggest that adaptations supporting the changes in excitability and resistance occur locally to these cells.", "contents": "Intracellularly studied excitability changes in coronal-pericruciate neurons following low frequency stimulation of the corticobulbar tract. Direct intracellular measurements of excitability changes to injected current were made in 116 cortical neurons of awake cats following antidromic or orthodromic activation by repetitive, 1-6 Hz stimulation of the pes pedunculi. The predominant effect seen in antidromically activated cells was a transient decrease in excitability and input resistance. The predominant effect seen in orthodromically (transsynaptically) activated cells was a transient increase in excitability and input resistance. In the absence of accompanying change in rates of spontaneous spike activity, the above results suggest that adaptations supporting the changes in excitability and resistance occur locally to these cells."}
{"id": "PMID:487221", "title": "Effects of damage to median raphe nucleus on ingestive behavior and wheel running activity.", "content": "The effects of damage to the median raphe nucleus on the ingestive behavior and wheel running activity of rats were studied. This nucleus was damaged by the placement of either electrolytic or chemical (5,7-dihydroxytryptamine) lesions. After the placement of either type of lesion, wheel running activity was significantly decreased for the duration of the 8 week post-operative period. Although there were transient decreases in both food and water intakes after damage to the median raphe nucleus, these decreases did not appear to result from impairments in neuro-regulatory mechanisms. Rather, the decrease in food intake seemed to be related to the decrease in locomotor activity, and the decrease in water intake appeared to be linked to the decrease in food intake. In some rats with electrolytic lesions in the median raphe nucleus, the decrease in water intake was followed by a transient period of hyperdipsia.", "contents": "Effects of damage to median raphe nucleus on ingestive behavior and wheel running activity. The effects of damage to the median raphe nucleus on the ingestive behavior and wheel running activity of rats were studied. This nucleus was damaged by the placement of either electrolytic or chemical (5,7-dihydroxytryptamine) lesions. After the placement of either type of lesion, wheel running activity was significantly decreased for the duration of the 8 week post-operative period. Although there were transient decreases in both food and water intakes after damage to the median raphe nucleus, these decreases did not appear to result from impairments in neuro-regulatory mechanisms. Rather, the decrease in food intake seemed to be related to the decrease in locomotor activity, and the decrease in water intake appeared to be linked to the decrease in food intake. In some rats with electrolytic lesions in the median raphe nucleus, the decrease in water intake was followed by a transient period of hyperdipsia."}
{"id": "PMID:487223", "title": "Depression of spontaneous and ionophore-induced transmitter release by ruthenium red at the neuromuscular junction.", "content": "The effects on spontaneous and ionophore-induced transmitter release of the inorganic dye, ruthenium red (RuR), a known inhibitor of calcium binding sites, were observed at the frog sartorius neuromuscular junction using intracellular recording techniques. Both crude and purified RuR, at concentrations of 1 and 5 micron depressed or blocked spontaneous release of acetylcholine (ACh) and reduced postsynaptic sensitivity to ACh, the crude dye being more potent than the pure. Pretreatment of muscles with RuR prevented the catastrophic reaction of junctions to 100 micron X537A ionophore. Increased levels of Ca2+ restored spontaneous transmitter release to control levels after depression or blockade by RuR. It was concluded that RuR blocks a critical membrane-bound binding site for calcium which is necessary for quantal release of transmitter.", "contents": "Depression of spontaneous and ionophore-induced transmitter release by ruthenium red at the neuromuscular junction. The effects on spontaneous and ionophore-induced transmitter release of the inorganic dye, ruthenium red (RuR), a known inhibitor of calcium binding sites, were observed at the frog sartorius neuromuscular junction using intracellular recording techniques. Both crude and purified RuR, at concentrations of 1 and 5 micron depressed or blocked spontaneous release of acetylcholine (ACh) and reduced postsynaptic sensitivity to ACh, the crude dye being more potent than the pure. Pretreatment of muscles with RuR prevented the catastrophic reaction of junctions to 100 micron X537A ionophore. Increased levels of Ca2+ restored spontaneous transmitter release to control levels after depression or blockade by RuR. It was concluded that RuR blocks a critical membrane-bound binding site for calcium which is necessary for quantal release of transmitter."}
{"id": "PMID:487229", "title": "Ventilation and chemoreflexes during enflurane sedation and anaesthesia in man.", "content": "Enflurane sedation and anaesthesia in healthy fit subjects reduced ventilation and the response to carbon dioxide, hypoxaemia and a low dose of doxapram, all in a dose-related fashion. Comparing the three chemoreflexes tested, the response to hypoxaemia and doxapram were the more profoundly impaired; they were nearly totally abolished by anaesthesia. These effects of enflurane on chemoreflex activities are qualitatively similar to those previously observed with halothane.", "contents": "Ventilation and chemoreflexes during enflurane sedation and anaesthesia in man. Enflurane sedation and anaesthesia in healthy fit subjects reduced ventilation and the response to carbon dioxide, hypoxaemia and a low dose of doxapram, all in a dose-related fashion. Comparing the three chemoreflexes tested, the response to hypoxaemia and doxapram were the more profoundly impaired; they were nearly totally abolished by anaesthesia. These effects of enflurane on chemoreflex activities are qualitatively similar to those previously observed with halothane."}
{"id": "PMID:487224", "title": "Impairments in saline intake induced by deposit-free electrolytic lesions in the thalamic gustatory area.", "content": "Previous studies demonstrating impairments in sodium appetite following destruction of the thalamic gustatory relay of the rat utilized lesion procedures which depositd irritative ferric ions around the lesion site. The present study used a deposit free lesioning procedure to determine whether the disruption in sodium appetite is attributable to factors other than tissue destruction. The results showed that deposite free lesions produce deficits in sodium appetite which are similar in specificity, intensity, and uniformity to those produced by irritative lesion procedures.", "contents": "Impairments in saline intake induced by deposit-free electrolytic lesions in the thalamic gustatory area. Previous studies demonstrating impairments in sodium appetite following destruction of the thalamic gustatory relay of the rat utilized lesion procedures which depositd irritative ferric ions around the lesion site. The present study used a deposit free lesioning procedure to determine whether the disruption in sodium appetite is attributable to factors other than tissue destruction. The results showed that deposite free lesions produce deficits in sodium appetite which are similar in specificity, intensity, and uniformity to those produced by irritative lesion procedures."}
{"id": "PMID:487230", "title": "Coaxial scavenger for paediatric anaesthesia.", "content": "A new scavenging device suitable for the Jackson-Rees modification to the Ayre's T-piece is described. This device maintains the essential components of the modification while effectively scavenging the circuit.", "contents": "Coaxial scavenger for paediatric anaesthesia. A new scavenging device suitable for the Jackson-Rees modification to the Ayre's T-piece is described. This device maintains the essential components of the modification while effectively scavenging the circuit."}
{"id": "PMID:487231", "title": "Reduction of nitrous oxide contamination in a paediatric hospital.", "content": "Ambient nitrous oxide concentrations were recorded in an operating room during delivery of the gas at low and medium flow-rates, with and without the application of simple scavenging devices. Residual background levels of nitrous oxide were still present more than one hour after disconnection of the flowmeters and use of the room. Scavenging reduced concentrations ten-fold. Adjacent corridors and the post-anaesthetic recovery room were contaminated with nitrous oxide from the operating rooms and from patients. A vigorous programme of checking for leaks and repairing and maintaining equipment, coupled with the use of suction scavenging, can reduce atmospheric contamination with nitrous oxide below 30 p.p.m., which is well within the limit suggested by the U.S. National Institute for Occupational Safety and Health.", "contents": "Reduction of nitrous oxide contamination in a paediatric hospital. Ambient nitrous oxide concentrations were recorded in an operating room during delivery of the gas at low and medium flow-rates, with and without the application of simple scavenging devices. Residual background levels of nitrous oxide were still present more than one hour after disconnection of the flowmeters and use of the room. Scavenging reduced concentrations ten-fold. Adjacent corridors and the post-anaesthetic recovery room were contaminated with nitrous oxide from the operating rooms and from patients. A vigorous programme of checking for leaks and repairing and maintaining equipment, coupled with the use of suction scavenging, can reduce atmospheric contamination with nitrous oxide below 30 p.p.m., which is well within the limit suggested by the U.S. National Institute for Occupational Safety and Health."}
{"id": "PMID:487232", "title": "[Blood and urinary cyanide concentrations during long-term sodium nitroprusside perfusion].", "content": "Five deeply comatose neurological patients were administered a continuous perfusion of sodium nitroprusside (SNP) at the rate of 3 microgram.kg-1.min-1. The levels of blood cyanide (CN-) were measured two hours after the start, then every 12 hours during, and 12 and 24 hours after the end of perfusion. The urinary output of CN- was also studied. The results show that total blood CN- stabilized after 36 hours to a mean value of 0.11 mg/l. When perfusion was stopped, CN- blood levels dropped but did not reach pre-perfusion values at the 24th hour. Urinary excretion of CN- which reached a maximum value of 0.050 mg/24 h represents a negligible amount and does not explain the fall of blood CN- and the occurrence of a concentration plateau. The results showing lower values obtained on non-anesthetized patients during the first hours of perfusion compared to those of a previous study done under neuroleptanaesthesia are discussed. These results suggest that prolonged perfusions at SNP at the rate of 0.177 mg.kg-1.h-1 do not produce toxic blood level of CN-.", "contents": "[Blood and urinary cyanide concentrations during long-term sodium nitroprusside perfusion]. Five deeply comatose neurological patients were administered a continuous perfusion of sodium nitroprusside (SNP) at the rate of 3 microgram.kg-1.min-1. The levels of blood cyanide (CN-) were measured two hours after the start, then every 12 hours during, and 12 and 24 hours after the end of perfusion. The urinary output of CN- was also studied. The results show that total blood CN- stabilized after 36 hours to a mean value of 0.11 mg/l. When perfusion was stopped, CN- blood levels dropped but did not reach pre-perfusion values at the 24th hour. Urinary excretion of CN- which reached a maximum value of 0.050 mg/24 h represents a negligible amount and does not explain the fall of blood CN- and the occurrence of a concentration plateau. The results showing lower values obtained on non-anesthetized patients during the first hours of perfusion compared to those of a previous study done under neuroleptanaesthesia are discussed. These results suggest that prolonged perfusions at SNP at the rate of 0.177 mg.kg-1.h-1 do not produce toxic blood level of CN-."}
{"id": "PMID:487233", "title": "Epidural analgesia and the metabolic response to surgery.", "content": "The effect of epidural blockade on the metabolic and hormonal responses to pelvic surgery was investigated in 14 female patients. Central venous blood samples were collected every 30 minutes and analysed for free fatty acids, glycerol, beta-hydroxybutyrate, acetoacetate, glucose, lactate, pyruvate, cortisol and growth hormone concentrations. There was no change in fat and glucose metabolism except for a transient decrease in lipolysis after 30 minutes of surgery. Cortisol and growth hormone values were significantly increased (p less than 0.01) after 60 minutes. A small but statistically significant increase in blood lactate concentration was observed (p less than 0.01) and the concept of a \"metabolic V/Q\" abnormality is postulated to explain the lacticacidaemia.", "contents": "Epidural analgesia and the metabolic response to surgery. The effect of epidural blockade on the metabolic and hormonal responses to pelvic surgery was investigated in 14 female patients. Central venous blood samples were collected every 30 minutes and analysed for free fatty acids, glycerol, beta-hydroxybutyrate, acetoacetate, glucose, lactate, pyruvate, cortisol and growth hormone concentrations. There was no change in fat and glucose metabolism except for a transient decrease in lipolysis after 30 minutes of surgery. Cortisol and growth hormone values were significantly increased (p less than 0.01) after 60 minutes. A small but statistically significant increase in blood lactate concentration was observed (p less than 0.01) and the concept of a \"metabolic V/Q\" abnormality is postulated to explain the lacticacidaemia."}
{"id": "PMID:487234", "title": "A comparison of the effects of continuous ketamine infusion and halothane on oxygenation during one-lung anaesthesia in dogs.", "content": "It has been shown that a continuous infusion of ketamine during one-lung anaesthesia combined with a 50 per cent oxygen-curare anaesthetic technique will provide consistently lower shunt fraction and higher Pao2 compared with halothane under the same experimental conditions. Because no additional factor was observed which could account for these changes and because the responses of the animals to the two anaesthetic agents were similar--the only difference being a different initial set point--the experimental model may be considered adequate. In the authors' view the difference in shunt fractions may be attributed to a more stable hypoxic reflex during ketamine anaesthesia. Further experimentation will be necessary to fully exclude the possibility of sequence-related changes affecting some of these results and to determine whether or not certain groups of dogs respond in a qualitatively different fashion.", "contents": "A comparison of the effects of continuous ketamine infusion and halothane on oxygenation during one-lung anaesthesia in dogs. It has been shown that a continuous infusion of ketamine during one-lung anaesthesia combined with a 50 per cent oxygen-curare anaesthetic technique will provide consistently lower shunt fraction and higher Pao2 compared with halothane under the same experimental conditions. Because no additional factor was observed which could account for these changes and because the responses of the animals to the two anaesthetic agents were similar--the only difference being a different initial set point--the experimental model may be considered adequate. In the authors' view the difference in shunt fractions may be attributed to a more stable hypoxic reflex during ketamine anaesthesia. Further experimentation will be necessary to fully exclude the possibility of sequence-related changes affecting some of these results and to determine whether or not certain groups of dogs respond in a qualitatively different fashion."}
{"id": "PMID:487236", "title": "Can naloxone inhibit the cardiovascular effect of acupuncture?", "content": "Forty dogs were studied during MAC-1 halothane anesthesia with a chronically implanted electromagnetic flow probe on the ascending aorta. Cardiac output, stroke volume, heart rate, mean arterial pressure, pulse pressure, central venous pressure and total peripheral resistance were determined in addition to Pao2, pH, Paco2 and base deficit. Acupuncture moxibustion with electrocautery at Jen Chung (Go-26) produced a significant (five per cent level) increase in the cardiac output, stroke volume, heart rate, mean arterial pressure and pulse pressure and a significant decrease in total peripheral resistance during a 120-minute period of observation in dogs under halothane anaesthesia. Acupuncture moxibustion at Go-26 following pretreatment with the narcotic antagonist naloxone (1 mg.kg-1) produced a significant increase in cardiac output, heart rate, mean arterial pressure. It is concluded that naloxone, which inhibits the analgesic effects of acupuncture, does not inhibit the symphathomimetic effect of acupuncture or moxibustion at Jen Chung (Go-26) in dogs under light MAC-1 halothane anaesthesia.", "contents": "Can naloxone inhibit the cardiovascular effect of acupuncture? Forty dogs were studied during MAC-1 halothane anesthesia with a chronically implanted electromagnetic flow probe on the ascending aorta. Cardiac output, stroke volume, heart rate, mean arterial pressure, pulse pressure, central venous pressure and total peripheral resistance were determined in addition to Pao2, pH, Paco2 and base deficit. Acupuncture moxibustion with electrocautery at Jen Chung (Go-26) produced a significant (five per cent level) increase in the cardiac output, stroke volume, heart rate, mean arterial pressure and pulse pressure and a significant decrease in total peripheral resistance during a 120-minute period of observation in dogs under halothane anaesthesia. Acupuncture moxibustion at Go-26 following pretreatment with the narcotic antagonist naloxone (1 mg.kg-1) produced a significant increase in cardiac output, heart rate, mean arterial pressure. It is concluded that naloxone, which inhibits the analgesic effects of acupuncture, does not inhibit the symphathomimetic effect of acupuncture or moxibustion at Jen Chung (Go-26) in dogs under light MAC-1 halothane anaesthesia."}
{"id": "PMID:487237", "title": "Ventricular fibrillation in a patient with unsuspected mitral valve prolapse and a prolonged Q-T interval.", "content": "Mitral valve prolapse is a common cardiac abnormality associated with arrhythmias and sudden death. In most instances it can be diagnosed on the basis of physical findings. Those patients who are symptomatic or who display electrocardiographic abnormalities appear to be most susceptible to arrhythmias and, therefore, may be at increased risk for anaesthesia. Because the syndrome is relatively common and may present a very innocent clinical picture, anaesthetists should be aware of this condition and the problems it may present. A case of mitral valve prolapse syndrome associated with ventricular fibrillation on induction of anaesthesia is reported. The symptoms and pathophysiology of the disorder are reviewed and the potential problems and the anaesthetic management are discussed.", "contents": "Ventricular fibrillation in a patient with unsuspected mitral valve prolapse and a prolonged Q-T interval. Mitral valve prolapse is a common cardiac abnormality associated with arrhythmias and sudden death. In most instances it can be diagnosed on the basis of physical findings. Those patients who are symptomatic or who display electrocardiographic abnormalities appear to be most susceptible to arrhythmias and, therefore, may be at increased risk for anaesthesia. Because the syndrome is relatively common and may present a very innocent clinical picture, anaesthetists should be aware of this condition and the problems it may present. A case of mitral valve prolapse syndrome associated with ventricular fibrillation on induction of anaesthesia is reported. The symptoms and pathophysiology of the disorder are reviewed and the potential problems and the anaesthetic management are discussed."}
{"id": "PMID:487238", "title": "Anaphylactoid reaction to mannitol.", "content": "A 16-year old boy with a lesion of the right eye developed, during the preoperative administration of a mannitol infusion, an anaphylactoid reaction characterized by hypotension, periorbital oedema and bronchospasm. This quickly resolved following cessation of the infusion and appropriate therapeutic measures. There were no long-lasting effects. We considered mannitol the causative agent because of its temporal relationship to the reaction and our inability to seriously implicate any other medication. A history of childhood atopy may have been a predisposing factor.", "contents": "Anaphylactoid reaction to mannitol. A 16-year old boy with a lesion of the right eye developed, during the preoperative administration of a mannitol infusion, an anaphylactoid reaction characterized by hypotension, periorbital oedema and bronchospasm. This quickly resolved following cessation of the infusion and appropriate therapeutic measures. There were no long-lasting effects. We considered mannitol the causative agent because of its temporal relationship to the reaction and our inability to seriously implicate any other medication. A history of childhood atopy may have been a predisposing factor."}
{"id": "PMID:487239", "title": "Unilateral pulmonary oedema: a case report.", "content": "We have presented a case of unilateral pulmonary oedema which we feel is secondary to obstruction of the right main bronchus during spontaneous respiration. An anode tracheal tube was introduced through a tracheostomy during operation in a man with pre-existing pulmonary and cardiac disease. At the end of the anaesthetic, acute obstruction to ventilation of the right main bronchus developed with the tube still in place and the patient breathing spontaneously. The obstruction was relieved, by repositioning of the tracheal tube, but not before the patient developed right unilateral pulmonary oedema.", "contents": "Unilateral pulmonary oedema: a case report. We have presented a case of unilateral pulmonary oedema which we feel is secondary to obstruction of the right main bronchus during spontaneous respiration. An anode tracheal tube was introduced through a tracheostomy during operation in a man with pre-existing pulmonary and cardiac disease. At the end of the anaesthetic, acute obstruction to ventilation of the right main bronchus developed with the tube still in place and the patient breathing spontaneously. The obstruction was relieved, by repositioning of the tracheal tube, but not before the patient developed right unilateral pulmonary oedema."}
{"id": "PMID:487241", "title": "The effect of wheat germ agglutinin on sialyl and galactosyltransferases of rat liver Golgi membranes.", "content": "The sialyltransferase and galactosyltransferase activities of the Golgi-rich fraction from rat liver were enhanced by the binding of wheat germ agglutinin (WGA). The sialytransferase was more sensitive than the galactosyltransferase to the WGA. Maximal stimulation of the galactosyltransferase activity resulted from the binding of 60--80 micrograms WGA to the Golgi membrane, while only 40 micrograms of WGA produced a maximal enhancement in the sialyltransferase activity. Within 5 min of WGA binding, the Golgi sialytransferase activity was doubled. After the initial binding of WGA to the Golgi fraction, the galactosyltransferase activity was decreased by 30%. However, in 15 min the activity was doubled by the binding of WGA. The activities of both enzymes were further enhanced by incubation for up to 90 min. The stimulation of both sialyltransferase and galactosyltransferase activities by WGA was reversed by N-acetyl-D-glucosamine (GlcNAc), the specific inhibitor of agglutination by WGA. Complete reversal of the enhanced activity was observed after 20--30 min in the presence of 1 micromol GlcNAc. The association constant for the binding of WGA to the Golgi membranes was calculated to be 4.16 X 10(-6) M from a Steck-Wallach plot. The 'n' value or mean binding sites was calculated as 5.26 X 10(-5) M/mg of Golgi membrane protein.", "contents": "The effect of wheat germ agglutinin on sialyl and galactosyltransferases of rat liver Golgi membranes. The sialyltransferase and galactosyltransferase activities of the Golgi-rich fraction from rat liver were enhanced by the binding of wheat germ agglutinin (WGA). The sialytransferase was more sensitive than the galactosyltransferase to the WGA. Maximal stimulation of the galactosyltransferase activity resulted from the binding of 60--80 micrograms WGA to the Golgi membrane, while only 40 micrograms of WGA produced a maximal enhancement in the sialyltransferase activity. Within 5 min of WGA binding, the Golgi sialytransferase activity was doubled. After the initial binding of WGA to the Golgi fraction, the galactosyltransferase activity was decreased by 30%. However, in 15 min the activity was doubled by the binding of WGA. The activities of both enzymes were further enhanced by incubation for up to 90 min. The stimulation of both sialyltransferase and galactosyltransferase activities by WGA was reversed by N-acetyl-D-glucosamine (GlcNAc), the specific inhibitor of agglutination by WGA. Complete reversal of the enhanced activity was observed after 20--30 min in the presence of 1 micromol GlcNAc. The association constant for the binding of WGA to the Golgi membranes was calculated to be 4.16 X 10(-6) M from a Steck-Wallach plot. The 'n' value or mean binding sites was calculated as 5.26 X 10(-5) M/mg of Golgi membrane protein."}
{"id": "PMID:487242", "title": "Proteolytic conversion of rat thymus DNA polymerase alpha to a more active form.", "content": "The relationship between two DNA polymerase alpha species from mammalian tissues has been resolved with the isolation of a protease from rat thymus which converts the larger alpha polymerase (7.3S) to a smaller (5.4S) size. The proteolytic activity is present only in the chromatin fraction and the limited proteolysis is accompanied by an increase in activity of the DNA polymerase, possibly consistent with a biological control function for this phenomenon.", "contents": "Proteolytic conversion of rat thymus DNA polymerase alpha to a more active form. The relationship between two DNA polymerase alpha species from mammalian tissues has been resolved with the isolation of a protease from rat thymus which converts the larger alpha polymerase (7.3S) to a smaller (5.4S) size. The proteolytic activity is present only in the chromatin fraction and the limited proteolysis is accompanied by an increase in activity of the DNA polymerase, possibly consistent with a biological control function for this phenomenon."}
{"id": "PMID:487243", "title": "Cell-free synthesis of rat liver zinc-thioneins.", "content": "The induction of rat liver zinc-thioneins mRNA was studied in a wheat germ cell-free translation system. Liver poly A rich polysomal RNA was isolated from rats which had been injected with zinc sulfate 5 h previously. These RNA preparations stimulated the incorporation of [35S]cystine into trichloroacetic acid insoluble proteins when assayed in the cell-free synthetic system. The translation products were characterized by Sephadex G-75 chromatography in 8 M urea--50 mM beta-mercaptoethanol, by disc gel electrophoresis in 4 M area--Tris-glycine buffer (pH 9.2), and by peptide fingerprinting with pepsin. These results were identical with authentic rat liver zinc-thioneins. The zinc-thioneins mRNA activity in the control rats, however, was minimal. The stimulation in zinc-thioneins synthesis observed in the cell-free synthesis was similar to the increased synthesis of these polypeptides in vivo.", "contents": "Cell-free synthesis of rat liver zinc-thioneins. The induction of rat liver zinc-thioneins mRNA was studied in a wheat germ cell-free translation system. Liver poly A rich polysomal RNA was isolated from rats which had been injected with zinc sulfate 5 h previously. These RNA preparations stimulated the incorporation of [35S]cystine into trichloroacetic acid insoluble proteins when assayed in the cell-free synthetic system. The translation products were characterized by Sephadex G-75 chromatography in 8 M urea--50 mM beta-mercaptoethanol, by disc gel electrophoresis in 4 M area--Tris-glycine buffer (pH 9.2), and by peptide fingerprinting with pepsin. These results were identical with authentic rat liver zinc-thioneins. The zinc-thioneins mRNA activity in the control rats, however, was minimal. The stimulation in zinc-thioneins synthesis observed in the cell-free synthesis was similar to the increased synthesis of these polypeptides in vivo."}
{"id": "PMID:487244", "title": "Interaction of spironolactone with rat skin androgen receptor.", "content": "Some characteristics of the dorsal skin cytoplasmic androgen receptor (AR) have been studied in male rats. The affinity constant, the binding specificity, and the sedimentation profile of the receptor have been found to be similar to the rat prostate AR. The measurement of the number of binding sites in various hormonal conditions (deprivation) led to the conclusion that this receptor was largely occupied by endogeneous hormones from gonadal and (or) adrenal sources. Administration of spironolactone or canrenone to 7-day-castrated rats was accompanied by a rapid and drastic decrease of available binding sites. This diminution was ascribed to the competitive inhibition of canrenone, the active in vivo metabolite of spironolactone. It is postulated that the antiandrogenic action of spironolactone, at the skin level, is mediated by canrenone which inhibits the formation of specific testosterone and (or) 5 alpha-dihydrotestosterone receptor complex in cytoplasm and consequently in nuclei.", "contents": "Interaction of spironolactone with rat skin androgen receptor. Some characteristics of the dorsal skin cytoplasmic androgen receptor (AR) have been studied in male rats. The affinity constant, the binding specificity, and the sedimentation profile of the receptor have been found to be similar to the rat prostate AR. The measurement of the number of binding sites in various hormonal conditions (deprivation) led to the conclusion that this receptor was largely occupied by endogeneous hormones from gonadal and (or) adrenal sources. Administration of spironolactone or canrenone to 7-day-castrated rats was accompanied by a rapid and drastic decrease of available binding sites. This diminution was ascribed to the competitive inhibition of canrenone, the active in vivo metabolite of spironolactone. It is postulated that the antiandrogenic action of spironolactone, at the skin level, is mediated by canrenone which inhibits the formation of specific testosterone and (or) 5 alpha-dihydrotestosterone receptor complex in cytoplasm and consequently in nuclei."}
{"id": "PMID:487245", "title": "Affinity chromatography of Ruta graveolens L. O-methyltransferases. Studies demonstrating the potential of the technique in the mechanistic investigation of O-methyltransferases.", "content": "Two discrete furanocoumarin (5- and 8-)O-methyltransferases and a caffeic acid 3-O-methyl-transferase from cell cultures of Ruta graveoleus L. have been copurified by affinity chromatography on 1,6-diaminohexane agarose (AH-Sepharose 4B) linked with S-adenosyl-L-homocysteine (SAH). The furanocoumarin O-methyltransferases, which transfer a methyl group from S-adenosyl-L-methionine (SAM) to the 5- or 8-hydroxyls of linear furanocoumarins, were not retarded by 5-(3-carboxypropanamido)-xanthotoxin (CPAX) immobilized to AH-Sepharose 4B, but addition of SAM to the irrigant buffer led to complete retardation of both enzymes on this affinity system. An analogous phenomenon was observed for the caffeic acid O-methyltransferase, with a ferulic acid ligand coupled to the same insoluble support. SAH was as effective as SAM in promoting binding of the furanocoumarin O-methyltransferases to CPAX and caffeic acid 3-O-methyltransferase to immobilized ferulic acid, respectively. The strong and specific adsorption of these enzymes was abolished by exclusion of SAM or SAH from the irrigant buffer. It is concluded that the enzymes bind first to SAM or SAH, and that this binding process in turn induces the binding site for their specific phenolic substrates or their analogs. Based on these findings, a compulsory-ordered kinetic mechanism for the action of these O-methyltransferases is postulated.", "contents": "Affinity chromatography of Ruta graveolens L. O-methyltransferases. Studies demonstrating the potential of the technique in the mechanistic investigation of O-methyltransferases. Two discrete furanocoumarin (5- and 8-)O-methyltransferases and a caffeic acid 3-O-methyl-transferase from cell cultures of Ruta graveoleus L. have been copurified by affinity chromatography on 1,6-diaminohexane agarose (AH-Sepharose 4B) linked with S-adenosyl-L-homocysteine (SAH). The furanocoumarin O-methyltransferases, which transfer a methyl group from S-adenosyl-L-methionine (SAM) to the 5- or 8-hydroxyls of linear furanocoumarins, were not retarded by 5-(3-carboxypropanamido)-xanthotoxin (CPAX) immobilized to AH-Sepharose 4B, but addition of SAM to the irrigant buffer led to complete retardation of both enzymes on this affinity system. An analogous phenomenon was observed for the caffeic acid O-methyltransferase, with a ferulic acid ligand coupled to the same insoluble support. SAH was as effective as SAM in promoting binding of the furanocoumarin O-methyltransferases to CPAX and caffeic acid 3-O-methyltransferase to immobilized ferulic acid, respectively. The strong and specific adsorption of these enzymes was abolished by exclusion of SAM or SAH from the irrigant buffer. It is concluded that the enzymes bind first to SAM or SAH, and that this binding process in turn induces the binding site for their specific phenolic substrates or their analogs. Based on these findings, a compulsory-ordered kinetic mechanism for the action of these O-methyltransferases is postulated."}
{"id": "PMID:487246", "title": "Mycotoxins in animal feedstuffs in Ontario: 1972 to 1977.", "content": "Records of the Toxicology Laboratory, Veterinary Services Branch, Ontario Ministry of Agriculture and Food, indicate that 277 of 2,022 specimens of feedstuffs submitted between October 1, 1972 and September 30, 1978 contained one or more mycotoxins. The data indicate that zearalenone is an important mycotoxin in the provincial corn crops and that its incidence fluctuates from year to year. The percentages of specimens containing zearalenone were 16.3 (1972), 4.1 (1973), 5.5 (1974), 22.4 (1975), 9.5 (1976) and 13.0 (1977). Aflatoxins, ochratoxins and T-2 toxin were found in some specimens but their incidence was low.", "contents": "Mycotoxins in animal feedstuffs in Ontario: 1972 to 1977. Records of the Toxicology Laboratory, Veterinary Services Branch, Ontario Ministry of Agriculture and Food, indicate that 277 of 2,022 specimens of feedstuffs submitted between October 1, 1972 and September 30, 1978 contained one or more mycotoxins. The data indicate that zearalenone is an important mycotoxin in the provincial corn crops and that its incidence fluctuates from year to year. The percentages of specimens containing zearalenone were 16.3 (1972), 4.1 (1973), 5.5 (1974), 22.4 (1975), 9.5 (1976) and 13.0 (1977). Aflatoxins, ochratoxins and T-2 toxin were found in some specimens but their incidence was low."}
{"id": "PMID:487247", "title": "Experimental bovine pneumonic pasteurellosis. I. Prevention of the disease.", "content": "Fifteen three- to six-month old Hereford-cross calves were divided into three groups. The first group was inoculated with bovid herpersvirus 1 (Strain 108), the second with a commercial intranasal vaccine against bovid herpesvirus 1 and the third group acted as controls. At least three weeks after vaccination, all calves were weaned, placed in an environmental chamber at 25.0 degrees C (days) and -13.3 degrees C (nights) and challenged with an aerosol of bovid herpesvirus 1 followed four days later by an aerosol of Pasteurella haemolytica. All surviving calves were sacrificed four days after the second aerosol. None of the calves inoculated with bovid herpesvirus 1 virus or the commercial vaccine developed a generalized pneumonia, although there were one or two nodules (4--8 mm diameter) in two of the calves given the commercial vaccine. Four of the five control calves had extensive lobar pneumonia at necropsy, two of the five died from the disease. Details of the clinical, pathological, bacteriological, virological and some of the serological findings are reported.", "contents": "Experimental bovine pneumonic pasteurellosis. I. Prevention of the disease. Fifteen three- to six-month old Hereford-cross calves were divided into three groups. The first group was inoculated with bovid herpersvirus 1 (Strain 108), the second with a commercial intranasal vaccine against bovid herpesvirus 1 and the third group acted as controls. At least three weeks after vaccination, all calves were weaned, placed in an environmental chamber at 25.0 degrees C (days) and -13.3 degrees C (nights) and challenged with an aerosol of bovid herpesvirus 1 followed four days later by an aerosol of Pasteurella haemolytica. All surviving calves were sacrificed four days after the second aerosol. None of the calves inoculated with bovid herpesvirus 1 virus or the commercial vaccine developed a generalized pneumonia, although there were one or two nodules (4--8 mm diameter) in two of the calves given the commercial vaccine. Four of the five control calves had extensive lobar pneumonia at necropsy, two of the five died from the disease. Details of the clinical, pathological, bacteriological, virological and some of the serological findings are reported."}
{"id": "PMID:487248", "title": "Experimental bovine pneumonic pasteurellosis. II. Genesis and prevention.", "content": "Two experiments were conducted. In the first, 16 crossbred Hereford calves were divided into two equal groups. The first group was vaccinated intranasally with a commercial vaccine against bovid herpesvirus 1 and the second group was unvaccinated. The calves were later exposed to an aerosol of bovid herpesvirus 1 (strain 108) for five minutes. Four calves from each group were subjected to transportation and four calves from each group were kept in an environmental chamber for four days. Four days after viral aerosol all calves were exposed to an aerosol of Pasteurella haemolytica and the same subgroups were again transported or held in the chamber for a further four days. The calves that did not die from pneumonia were necropsied ten days after the final day of transport. Pulmonary lesions were present in both vaccinated and control animals but were less extensive in the vaccinated calves. Six of eight vaccinated but none of the eight control calves survived. In the second experiment, eight crossbred Hereford calves were divided into two equal groups. One group was vaccinated with bovid herpesvirus 1 (strain 108) and the other acted as controls. Four weeks later all calves were sequentially exposed to aerosols of bovid herpesvirus 1 (strain 108) and P. haemolytica four days apart. Three of the four controls but none of the vaccinates died from pneumonia. Every lobe of the lungs in all the controls was affected by pneumonia while no pulmonary lesions were found in the vaccinated calves. The differences in efficacy of the modes of vaccination and the possible role of transport stress are discussed.", "contents": "Experimental bovine pneumonic pasteurellosis. II. Genesis and prevention. Two experiments were conducted. In the first, 16 crossbred Hereford calves were divided into two equal groups. The first group was vaccinated intranasally with a commercial vaccine against bovid herpesvirus 1 and the second group was unvaccinated. The calves were later exposed to an aerosol of bovid herpesvirus 1 (strain 108) for five minutes. Four calves from each group were subjected to transportation and four calves from each group were kept in an environmental chamber for four days. Four days after viral aerosol all calves were exposed to an aerosol of Pasteurella haemolytica and the same subgroups were again transported or held in the chamber for a further four days. The calves that did not die from pneumonia were necropsied ten days after the final day of transport. Pulmonary lesions were present in both vaccinated and control animals but were less extensive in the vaccinated calves. Six of eight vaccinated but none of the eight control calves survived. In the second experiment, eight crossbred Hereford calves were divided into two equal groups. One group was vaccinated with bovid herpesvirus 1 (strain 108) and the other acted as controls. Four weeks later all calves were sequentially exposed to aerosols of bovid herpesvirus 1 (strain 108) and P. haemolytica four days apart. Three of the four controls but none of the vaccinates died from pneumonia. Every lobe of the lungs in all the controls was affected by pneumonia while no pulmonary lesions were found in the vaccinated calves. The differences in efficacy of the modes of vaccination and the possible role of transport stress are discussed."}
{"id": "PMID:487249", "title": "Bovine and equine onchocerciasis in eastern North America with a discussion on cuticular morphology of Onchocerca spp. in cattle.", "content": "Skin sections and/or the ligamentum nuchae and ligamentum gastrolienale were examined from twelve bovine carcasses obtained from southern and eastern Ontario and from Quebec. Of these, seven were shown to be infected with Onchocerca gutturosa and/or Onchocerca lienalis. The morphology of the adult female cuticle is discussed. Skin sections from 43 equine carcasses from a slaughter house in Grenville, Quebec were examined and microfilariae of Onchocerca sp. were recovered from 32 (74%). There are probably referable to Onchocerca cervicalis.", "contents": "Bovine and equine onchocerciasis in eastern North America with a discussion on cuticular morphology of Onchocerca spp. in cattle. Skin sections and/or the ligamentum nuchae and ligamentum gastrolienale were examined from twelve bovine carcasses obtained from southern and eastern Ontario and from Quebec. Of these, seven were shown to be infected with Onchocerca gutturosa and/or Onchocerca lienalis. The morphology of the adult female cuticle is discussed. Skin sections from 43 equine carcasses from a slaughter house in Grenville, Quebec were examined and microfilariae of Onchocerca sp. were recovered from 32 (74%). There are probably referable to Onchocerca cervicalis."}
{"id": "PMID:487250", "title": "A syndrome of hereditary tyrosinemia in mink (Mustela vison Schreb.).", "content": "A hereditary disease in mink (Mustela vison Schreb.) leading to death when the affected kits are about six weeks old has been investigated. The disorder is inherited as a simple autosomal recessive character. Strongly elevated plasma tyrosine concentration is an outstanding feature of the disease. An enzyme defect in tyrosine aminotransferase (EC 2.6.1.5) or 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) is considered together with the possibility of a parallel between the disease in mink and the disease tyrosinosis or hereditary tyrosinemia in man.", "contents": "A syndrome of hereditary tyrosinemia in mink (Mustela vison Schreb.). A hereditary disease in mink (Mustela vison Schreb.) leading to death when the affected kits are about six weeks old has been investigated. The disorder is inherited as a simple autosomal recessive character. Strongly elevated plasma tyrosine concentration is an outstanding feature of the disease. An enzyme defect in tyrosine aminotransferase (EC 2.6.1.5) or 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) is considered together with the possibility of a parallel between the disease in mink and the disease tyrosinosis or hereditary tyrosinemia in man."}
{"id": "PMID:487251", "title": "Probstmayria vivipara pinworms in ponies.", "content": "From 1967--1978 observations were made on the presence of the small equine pinworm, Probstmayria vivipara, in seven experimental ponies. The life cycle of this nematode is unusual in that it is endogenous with development of all stages occurring within the host's digestive tract. Initially, worms were found in the feces of four of seven ponies following treatment with thiabendazole but the infection was later transmitted to all ponies possibly via coprophagy. Still later, based on fecal and postmortem examinations, four of the seven ponies lost their pinworm burdens. At necropsy, the principal sites of infection were observed to be the cecum and right ventral colon. Despite the large number of pinworms present, clinical signs were not observed.", "contents": "Probstmayria vivipara pinworms in ponies. From 1967--1978 observations were made on the presence of the small equine pinworm, Probstmayria vivipara, in seven experimental ponies. The life cycle of this nematode is unusual in that it is endogenous with development of all stages occurring within the host's digestive tract. Initially, worms were found in the feces of four of seven ponies following treatment with thiabendazole but the infection was later transmitted to all ponies possibly via coprophagy. Still later, based on fecal and postmortem examinations, four of the seven ponies lost their pinworm burdens. At necropsy, the principal sites of infection were observed to be the cecum and right ventral colon. Despite the large number of pinworms present, clinical signs were not observed."}
{"id": "PMID:487252", "title": "Acquired retinal macroaneurysms.", "content": "Five patients with retinal macroaneurysms are described. The aneurysms may be an incidental finding or they may cause visual loss from retinal, preretinal and vitreous hemorrhages or from macular edema or exudation. The prognosis is good in those cases presenting with hemorrhage but is poorer in those with macular problems. Acquired aneurysms are found in older patients who are hypertensive or have vascular disease. We believe that retinal emboli play an important part in their development.", "contents": "Acquired retinal macroaneurysms. Five patients with retinal macroaneurysms are described. The aneurysms may be an incidental finding or they may cause visual loss from retinal, preretinal and vitreous hemorrhages or from macular edema or exudation. The prognosis is good in those cases presenting with hemorrhage but is poorer in those with macular problems. Acquired aneurysms are found in older patients who are hypertensive or have vascular disease. We believe that retinal emboli play an important part in their development."}
{"id": "PMID:487253", "title": "How do tears drain? Technetium studies.", "content": "We examined the tear drainage system of twenty-fiv normal volunteers by scintillography with Technetium Tc 99m (Pertechnetate). We photographed the changes occurring in the lacrimal sac and nasolacrimal duct during closure and opening of the eyelids. Our findings support Jones' views of normal tear drainage. The tracer was concentrated in the lacrimal sac while the lids were closed for 3 minutes and descended into the nasolacrimal duct as soon as they opened.", "contents": "How do tears drain? Technetium studies. We examined the tear drainage system of twenty-fiv normal volunteers by scintillography with Technetium Tc 99m (Pertechnetate). We photographed the changes occurring in the lacrimal sac and nasolacrimal duct during closure and opening of the eyelids. Our findings support Jones' views of normal tear drainage. The tracer was concentrated in the lacrimal sac while the lids were closed for 3 minutes and descended into the nasolacrimal duct as soon as they opened."}
{"id": "PMID:487254", "title": "The water provocative test in preglaucomatous chickens.", "content": "Our ability to induce glaucoma in birds provides an experimental system for exploring the mechanisms involved in the provocative water test. Waters loads of a level comparable to those used clinically produced larger intraocular pressure (IOP) changes in preglaucomatous and glaucomatous birds than in control birds. The magnitude of the response corresponded to the level of IOP before testing, that is, to the severity of the glaucoma. Changes in systemic blood pressure were not involved in the response. While hyotonicity of plasma may be the systemic determinant, the normal range of variation in this factor does not allow us at present to consider this a causal relationship.", "contents": "The water provocative test in preglaucomatous chickens. Our ability to induce glaucoma in birds provides an experimental system for exploring the mechanisms involved in the provocative water test. Waters loads of a level comparable to those used clinically produced larger intraocular pressure (IOP) changes in preglaucomatous and glaucomatous birds than in control birds. The magnitude of the response corresponded to the level of IOP before testing, that is, to the severity of the glaucoma. Changes in systemic blood pressure were not involved in the response. While hyotonicity of plasma may be the systemic determinant, the normal range of variation in this factor does not allow us at present to consider this a causal relationship."}
{"id": "PMID:487255", "title": "Does a barbiturate drug given in lethal dose enter the cornea?", "content": "We wished to see whether sodium phenobarbital (one of the commonest drugs used to commit suicide) given in a lethal dose would enter the cornea. Guinea pigs and rabbits were killed with an overdose of the drug. Using gas chromatography we were able to detect the drug not only in the vascular ocular tissues and the intraocular fluids but also in the avascular tissues such as the cornea.", "contents": "Does a barbiturate drug given in lethal dose enter the cornea? We wished to see whether sodium phenobarbital (one of the commonest drugs used to commit suicide) given in a lethal dose would enter the cornea. Guinea pigs and rabbits were killed with an overdose of the drug. Using gas chromatography we were able to detect the drug not only in the vascular ocular tissues and the intraocular fluids but also in the avascular tissues such as the cornea."}
{"id": "PMID:487256", "title": "The anticomplementary activity of the rabbit lens.", "content": "We wished to determine if inhibitors of the complement system are present in the normal rabbit lens. Soluble less extract in various dilutions was incubated with normal human serum (as source of complement). The residual hemolytic activity was measured using sheep erythrocytes sensitized with antisheep rabbit hemolysin. The lens extract was found to contain two heat stable anticomplementary factors of different molecular weights capable of cleaving C2 and C4. Gel filtration showed that one of these factors was located in the alpha-crystalline region. It is suggested that the anticomplementary factors in the lens may be related to a natural mechanism for the modulation of complement mediated lens injury.", "contents": "The anticomplementary activity of the rabbit lens. We wished to determine if inhibitors of the complement system are present in the normal rabbit lens. Soluble less extract in various dilutions was incubated with normal human serum (as source of complement). The residual hemolytic activity was measured using sheep erythrocytes sensitized with antisheep rabbit hemolysin. The lens extract was found to contain two heat stable anticomplementary factors of different molecular weights capable of cleaving C2 and C4. Gel filtration showed that one of these factors was located in the alpha-crystalline region. It is suggested that the anticomplementary factors in the lens may be related to a natural mechanism for the modulation of complement mediated lens injury."}
{"id": "PMID:487257", "title": "Falciform fold of retina.", "content": "A boy of 2 had evidence of congenital retinal non-attachment. The mother had a veil like fold in one eye. There was no evidence of any systemic disorder. The inheritance might be X-linked recessive. Mild manifestations of the disease in the carrier mother may be more frequent than has been assumed and should be looked for.", "contents": "Falciform fold of retina. A boy of 2 had evidence of congenital retinal non-attachment. The mother had a veil like fold in one eye. There was no evidence of any systemic disorder. The inheritance might be X-linked recessive. Mild manifestations of the disease in the carrier mother may be more frequent than has been assumed and should be looked for."}
{"id": "PMID:487258", "title": "The physician, the university and the community.", "content": "A physician is one who practises the healing art, including medicine and surgery -- is that enough?  The university is an institution for the promotion of education in the higher branches of learning -- is that enough?  The community, which requires both and supports both, has shown signs of discontent which we must appraise and appreciate. Ophthalmology, which has often led the way in the past in scientific endeavour, now has an opportunity to lead again, into areas of need in the community.", "contents": "The physician, the university and the community. A physician is one who practises the healing art, including medicine and surgery -- is that enough?  The university is an institution for the promotion of education in the higher branches of learning -- is that enough?  The community, which requires both and supports both, has shown signs of discontent which we must appraise and appreciate. Ophthalmology, which has often led the way in the past in scientific endeavour, now has an opportunity to lead again, into areas of need in the community."}
{"id": "PMID:487260", "title": "[Timolol maleate. Pharmacology and review of the literature].", "content": "The sympathetic system is composed of alpha and beta receptors. Beta blockers block the beta effect competitively. They slow the heart and decrease its contractile strength. They block bronchodilatation and the vasodillation of muscular arteries. Timolol is a non specific beta blocker. It decreases intraocular pressure by reducing aqueous humor formation. It does not change outflow facility. Its action starts twenty minutes after a topical drop, is maximum two to four hours later and lasts 24 hours and more. Timolol o.50% seems equivalent to pilocarpine 4% while Timolol 0.25% seems equivalent to epinephrine 2%. There is a further drop in pressure when added to pilocarpine, diamox as maximum medical treatment. Tables show its advantages, drawback, adverse reactions, and precautions to be taken.", "contents": "[Timolol maleate. Pharmacology and review of the literature]. The sympathetic system is composed of alpha and beta receptors. Beta blockers block the beta effect competitively. They slow the heart and decrease its contractile strength. They block bronchodilatation and the vasodillation of muscular arteries. Timolol is a non specific beta blocker. It decreases intraocular pressure by reducing aqueous humor formation. It does not change outflow facility. Its action starts twenty minutes after a topical drop, is maximum two to four hours later and lasts 24 hours and more. Timolol o.50% seems equivalent to pilocarpine 4% while Timolol 0.25% seems equivalent to epinephrine 2%. There is a further drop in pressure when added to pilocarpine, diamox as maximum medical treatment. Tables show its advantages, drawback, adverse reactions, and precautions to be taken."}
{"id": "PMID:487261", "title": "Effect of testosterone and 6-hydroxydopamine treatment on the metabolism of catecholamine and 5-hydroxytryptamine in methylcholanthrene-induced prostate carcinoma of rats.", "content": "The precursors tyrosine and tryptophan as well as the synthesizing and deaminating enzymes of catecholamines have been identified in methylcholanthrene-induced prostatic carcinoma of rats. Tyrosine hydroxylase, monoamine oxidase, catechol O-methyltransferase, dopamine, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid seemed to be neoplastic in origin, since electron microscopic studies failed to reveal the presence of any neuronal elements in this squamous epithelial cell carcinoma. Castration of rats significantly reduced the activity of tyrosine hydroxylase and the levels of tyrosine, dopamine, tryptophan, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in prostate tumors. The changes appeared to be androgen specific since reintroduction of testosterone restored several of these biochemical parameters virtually to control limits. Chemical sympathectomy induced by 6-hydroxydopamine failed to alter monoamine metabolism; however, the prostatic tumor grown in 6-hydroxydopamine-treated rats showed significantly (32%) less necrosis than those grown in normal animals.", "contents": "Effect of testosterone and 6-hydroxydopamine treatment on the metabolism of catecholamine and 5-hydroxytryptamine in methylcholanthrene-induced prostate carcinoma of rats. The precursors tyrosine and tryptophan as well as the synthesizing and deaminating enzymes of catecholamines have been identified in methylcholanthrene-induced prostatic carcinoma of rats. Tyrosine hydroxylase, monoamine oxidase, catechol O-methyltransferase, dopamine, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid seemed to be neoplastic in origin, since electron microscopic studies failed to reveal the presence of any neuronal elements in this squamous epithelial cell carcinoma. Castration of rats significantly reduced the activity of tyrosine hydroxylase and the levels of tyrosine, dopamine, tryptophan, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in prostate tumors. The changes appeared to be androgen specific since reintroduction of testosterone restored several of these biochemical parameters virtually to control limits. Chemical sympathectomy induced by 6-hydroxydopamine failed to alter monoamine metabolism; however, the prostatic tumor grown in 6-hydroxydopamine-treated rats showed significantly (32%) less necrosis than those grown in normal animals."}
{"id": "PMID:487262", "title": "Vagal inhibitory effects on peripheral circulation in acute coronary occlusion.", "content": "The reflex adjustments of the peripheral circulation in response to acute coronary occlusion were studied in anesthetized dogs with isolated vascular beds perfused at constant flow. Coronary occlusion caused significant increases in perfusion pressure which averaged 27 +/- 4 mmHg in the hindlimb, 19 +/- 8 mmHg in skeletal muscle, and 13 + 5 mmHg in the mesenteric artery. These responses were less than half those caused by a similar decrease in aortic pressure obtained with hemorrhage. Coronary occlusion caused no significant changes in renal and paw circulations, while marked vasoconstriction resulted from hemorrhage. When aortic pressure was maintained constant throughout the duration of coronary occlusion, there was a significant vasodilatation in all beds studied. After vagotomy, coronary occlusion caused a constrictor response similar in magnitude to that caused by hemorrhage in each vascular bed and the dilator responses to occlusion at constant aortic pressure were abolished. Both constrictor and dilator changes were prevented by alpha-adrenergic blockade. Mechanical distension of the left ventricle in four dogs with carotid sinus nerves cut caused a significant reflexdilatation in the hindlimb. Thus, coronary occlusion initiates an inhibitory reflex mediated by vagal afferents which opposes peripheral vasoconstriction most effectively in the renal and paw circulations.", "contents": "Vagal inhibitory effects on peripheral circulation in acute coronary occlusion. The reflex adjustments of the peripheral circulation in response to acute coronary occlusion were studied in anesthetized dogs with isolated vascular beds perfused at constant flow. Coronary occlusion caused significant increases in perfusion pressure which averaged 27 +/- 4 mmHg in the hindlimb, 19 +/- 8 mmHg in skeletal muscle, and 13 + 5 mmHg in the mesenteric artery. These responses were less than half those caused by a similar decrease in aortic pressure obtained with hemorrhage. Coronary occlusion caused no significant changes in renal and paw circulations, while marked vasoconstriction resulted from hemorrhage. When aortic pressure was maintained constant throughout the duration of coronary occlusion, there was a significant vasodilatation in all beds studied. After vagotomy, coronary occlusion caused a constrictor response similar in magnitude to that caused by hemorrhage in each vascular bed and the dilator responses to occlusion at constant aortic pressure were abolished. Both constrictor and dilator changes were prevented by alpha-adrenergic blockade. Mechanical distension of the left ventricle in four dogs with carotid sinus nerves cut caused a significant reflexdilatation in the hindlimb. Thus, coronary occlusion initiates an inhibitory reflex mediated by vagal afferents which opposes peripheral vasoconstriction most effectively in the renal and paw circulations."}
{"id": "PMID:487263", "title": "Teratogenic effects of diazepam in the hamster.", "content": "Pregnant hamsters were treated with different doses of oral and intravenous diazepam during the period of organogenesis. Teratogenic effects of diazepam were observed following oral treatment on days 8 and 10 and following intravenous treatment on day 11 of gestation. Types of malformations included cleft palate, exencephaly, limb anomalies, and hemorrhage. A dose-effect relationship was not observed. Comparison with reported literature seems to indicate that diazepam may be a mild teratogen in some species.", "contents": "Teratogenic effects of diazepam in the hamster. Pregnant hamsters were treated with different doses of oral and intravenous diazepam during the period of organogenesis. Teratogenic effects of diazepam were observed following oral treatment on days 8 and 10 and following intravenous treatment on day 11 of gestation. Types of malformations included cleft palate, exencephaly, limb anomalies, and hemorrhage. A dose-effect relationship was not observed. Comparison with reported literature seems to indicate that diazepam may be a mild teratogen in some species."}
{"id": "PMID:487264", "title": "Structure-activity studies of [des-Arg9]-bradykinin on the B1 receptor of the rabbit aorta.", "content": "Eight L-alanine analogues of [des-Arg9]-bradykinin and a few other compounds substituted in positions 5 and (or) 8 have been tested on rabbit aortic strips in order to identify the group(s) responsible for binding and (or) stimulation of the B1 receptor. The results obtained with the L-Ala series have shown that the active group is located at the C-terminal end and it is probably Phe8, while the middle part and the N-terminal end of the peptide molecule are primarily involved in binding the agonist to the receptor. An aromatic ring is required in position 8 for activation of receptors, since the elimination or aromaticity (as in [Leu8,des-Arg9]-bradykinin and in [cyclohexylalanine8,des-Arg9]-bradykinin) brings about pure and competitive antagonists. Some compounds exert an angiotensin-like effect when applied at very high concentrations.", "contents": "Structure-activity studies of [des-Arg9]-bradykinin on the B1 receptor of the rabbit aorta. Eight L-alanine analogues of [des-Arg9]-bradykinin and a few other compounds substituted in positions 5 and (or) 8 have been tested on rabbit aortic strips in order to identify the group(s) responsible for binding and (or) stimulation of the B1 receptor. The results obtained with the L-Ala series have shown that the active group is located at the C-terminal end and it is probably Phe8, while the middle part and the N-terminal end of the peptide molecule are primarily involved in binding the agonist to the receptor. An aromatic ring is required in position 8 for activation of receptors, since the elimination or aromaticity (as in [Leu8,des-Arg9]-bradykinin and in [cyclohexylalanine8,des-Arg9]-bradykinin) brings about pure and competitive antagonists. Some compounds exert an angiotensin-like effect when applied at very high concentrations."}
{"id": "PMID:487265", "title": "Effect of isoproterenol on phosphorylase activation in the hyperthyroid rat heart.", "content": "Pretreatment of rats for 3 days with triiodothyronine produced an increase in rate in the right atrium and a decrease in force of contraction in the right ventricle and Langendorff heart. Isoproterenol administration produced a time-dependent increase in rate and tension. The increase in rate was consistently greater in atria from hyperthyroid rats, and the increase in tension consistently greater in tissues from euthyroid rats. Isoproterenol also produced a time- and dose-dependent increase in phosphorylase a activity. In the isolated atria and ventricles enzyme activity was similar in the two groups. In the Langendorff hearts, however, there was an enhancement of the isoproterenol-induced increase in phosphorylase activity in hearts from hyperthyroid rats. Reduction of the coronary blood flow to the level found in euthyroid animals did not reduce the potentiation of phosphorylase activation found in hearts from hyperthyroid rats. It is concluded that the potentiation of phosphorylase activation in hearts from hyperthyroid rats is not due to the increase in coronary blood flow.", "contents": "Effect of isoproterenol on phosphorylase activation in the hyperthyroid rat heart. Pretreatment of rats for 3 days with triiodothyronine produced an increase in rate in the right atrium and a decrease in force of contraction in the right ventricle and Langendorff heart. Isoproterenol administration produced a time-dependent increase in rate and tension. The increase in rate was consistently greater in atria from hyperthyroid rats, and the increase in tension consistently greater in tissues from euthyroid rats. Isoproterenol also produced a time- and dose-dependent increase in phosphorylase a activity. In the isolated atria and ventricles enzyme activity was similar in the two groups. In the Langendorff hearts, however, there was an enhancement of the isoproterenol-induced increase in phosphorylase activity in hearts from hyperthyroid rats. Reduction of the coronary blood flow to the level found in euthyroid animals did not reduce the potentiation of phosphorylase activation found in hearts from hyperthyroid rats. It is concluded that the potentiation of phosphorylase activation in hearts from hyperthyroid rats is not due to the increase in coronary blood flow."}
{"id": "PMID:487266", "title": "Electrical coupling in the circular muscles of dog jejunum.", "content": "Two distinct layers of circular muscle have previously been demonstrated in dog jejunum, the main circular layer containing many gap junction contacts, and an inner dense muscle layer where no gap junctions have been found. Length constants were determined for these muscle layers and no significant difference was found between these values. The main circular muscle cells had lower membrane potentials and may have had abnormally low space constants owing to injury. It was concluded that the absence of gap junctions in the inner dense layer does not reduce the spread of passive current as might be expected of electrically isolated cells, and it is suggested that an alternate pathway for passive current exists in this layer.", "contents": "Electrical coupling in the circular muscles of dog jejunum. Two distinct layers of circular muscle have previously been demonstrated in dog jejunum, the main circular layer containing many gap junction contacts, and an inner dense muscle layer where no gap junctions have been found. Length constants were determined for these muscle layers and no significant difference was found between these values. The main circular muscle cells had lower membrane potentials and may have had abnormally low space constants owing to injury. It was concluded that the absence of gap junctions in the inner dense layer does not reduce the spread of passive current as might be expected of electrically isolated cells, and it is suggested that an alternate pathway for passive current exists in this layer."}
{"id": "PMID:487267", "title": "High-affinity choline uptake in regions of rat brain and the effect of antidepressants.", "content": "The effect of tricyclic antidepressants, chlorpromazine, and some monoamine oxidase inhibitors on the accumulation of [14C]choline by crude synaptosomal (P2) fraction from different regions of rat brain (cortex, striatum, and hippocampus) was investigated. Analysis of choline uptake kinetics resulted in high- and low-affinity components with different Michaelis constants. All tricyclic antidepressants tested inhibited in a dose-dependent manner the high-affinity choline uptake in the three regions, amitriptyline being the most potent. The IC50 values correlated significantly with the relative potencies of imipramine congeners in binding to muscarinic receptors in the brain. Neither tranylcypromine nor pargyline in concentrations up to 0.1 mM had any effect on choline transport. Concentrations of tricyclic antidepressants effective in inhibiting the uptake of choline failed to influence significantly the activity of choline acetyltransferase in brain regions examined. The results suggest that the effect of imipramine congeners on high-affinity choline uptake may be reflected in the anticholinergic properties of these compounds.", "contents": "High-affinity choline uptake in regions of rat brain and the effect of antidepressants. The effect of tricyclic antidepressants, chlorpromazine, and some monoamine oxidase inhibitors on the accumulation of [14C]choline by crude synaptosomal (P2) fraction from different regions of rat brain (cortex, striatum, and hippocampus) was investigated. Analysis of choline uptake kinetics resulted in high- and low-affinity components with different Michaelis constants. All tricyclic antidepressants tested inhibited in a dose-dependent manner the high-affinity choline uptake in the three regions, amitriptyline being the most potent. The IC50 values correlated significantly with the relative potencies of imipramine congeners in binding to muscarinic receptors in the brain. Neither tranylcypromine nor pargyline in concentrations up to 0.1 mM had any effect on choline transport. Concentrations of tricyclic antidepressants effective in inhibiting the uptake of choline failed to influence significantly the activity of choline acetyltransferase in brain regions examined. The results suggest that the effect of imipramine congeners on high-affinity choline uptake may be reflected in the anticholinergic properties of these compounds."}
{"id": "PMID:487268", "title": "Central antihypertensive properties of muscimol and related gamma-aminobutyric acid agonists and the interaction of muscimol with baroreceptor reflexes.", "content": "The central antihypertensive properties of four gamma-aminobutyric acid (GABA) analogs were characterized in anesthetized cats with implanted intracerebroventricular cannulae. An intracerebroventricular infusion (icv) of muscimol, 0.1--0.5 microgram/min (total dose: 1--5 micrograms, icv), substantially reduced mean arterial pressure and slightly reduced heart rate. The compound was not hypotensive at 5 micrograms, iv (total dose) and only slightly hypotensive after an intracisternal injection (5 micrograms). Kojic amine (2-aminomethyl-5-hydroxy-4H-pyran-4-one) and baclofen were also hypotensive following an intracerebroventricular infusion, but they were less active than muscimol. GABA, at 15--150 micrograms/min, icv (total dose, 150--1500 micrograms, icv), was not hypotensive by itself and unlike muscimol its activity was not enhanced in cats pretreated with nipecotic acid, an uptake inhibitor of GABA. The ability of muscimol to interfere with baroreceptor reflexes was considered in experiments in which reflex vasoconstrictor (carotid occlusion) and reflex vasodilatation (acute elevation in mean arterial pressure with norepinephrine) was measured in the perfused hindlimb of cats previously prepared with intracerebroventricular cannulae. Muscimol significantly attenuated the response to bilateral carotid occlusion and completely abolished reflex vasodilatation. The results suggest that GABA agonists and analogs may regulate blood pressure centrally and, through an interaction with the central nervous system, may attenuate baroreceptor reflexes.", "contents": "Central antihypertensive properties of muscimol and related gamma-aminobutyric acid agonists and the interaction of muscimol with baroreceptor reflexes. The central antihypertensive properties of four gamma-aminobutyric acid (GABA) analogs were characterized in anesthetized cats with implanted intracerebroventricular cannulae. An intracerebroventricular infusion (icv) of muscimol, 0.1--0.5 microgram/min (total dose: 1--5 micrograms, icv), substantially reduced mean arterial pressure and slightly reduced heart rate. The compound was not hypotensive at 5 micrograms, iv (total dose) and only slightly hypotensive after an intracisternal injection (5 micrograms). Kojic amine (2-aminomethyl-5-hydroxy-4H-pyran-4-one) and baclofen were also hypotensive following an intracerebroventricular infusion, but they were less active than muscimol. GABA, at 15--150 micrograms/min, icv (total dose, 150--1500 micrograms, icv), was not hypotensive by itself and unlike muscimol its activity was not enhanced in cats pretreated with nipecotic acid, an uptake inhibitor of GABA. The ability of muscimol to interfere with baroreceptor reflexes was considered in experiments in which reflex vasoconstrictor (carotid occlusion) and reflex vasodilatation (acute elevation in mean arterial pressure with norepinephrine) was measured in the perfused hindlimb of cats previously prepared with intracerebroventricular cannulae. Muscimol significantly attenuated the response to bilateral carotid occlusion and completely abolished reflex vasodilatation. The results suggest that GABA agonists and analogs may regulate blood pressure centrally and, through an interaction with the central nervous system, may attenuate baroreceptor reflexes."}
{"id": "PMID:487269", "title": "Alterations in ventilation and gas exchange during exercise-induced carbohydrate depletion.", "content": "The relationship between ventilation (VE), oxygen consumption (VO2), and carbon dioxide production (VCO2) during work were studied in four trained males during exercise-induced carbohydrate depletion. Repeated bouts of heavy treadmill exercise (6 min at 95% VO2 max) were performed once per hour for 24 h in order to promote a shift in energy substrate from carbohydrate to fat. Measurements of VO2 and VCO2 recorded during each minute indicated that VO2 was unaffected by the number of runs, whereas VCO2 showed a progressive reduction which amounted to 24% during the final run. A corresponding decline of 19% was observed in the respiratory exchange ratio. No significant change in VE occurred between any of the runs. It is concluded that during heavy, repeated, muscular exercise, reductions in VO2, strongly suggestive of an increased fat oxidation, are not accompanied by a corresponding change in ventilation.", "contents": "Alterations in ventilation and gas exchange during exercise-induced carbohydrate depletion. The relationship between ventilation (VE), oxygen consumption (VO2), and carbon dioxide production (VCO2) during work were studied in four trained males during exercise-induced carbohydrate depletion. Repeated bouts of heavy treadmill exercise (6 min at 95% VO2 max) were performed once per hour for 24 h in order to promote a shift in energy substrate from carbohydrate to fat. Measurements of VO2 and VCO2 recorded during each minute indicated that VO2 was unaffected by the number of runs, whereas VCO2 showed a progressive reduction which amounted to 24% during the final run. A corresponding decline of 19% was observed in the respiratory exchange ratio. No significant change in VE occurred between any of the runs. It is concluded that during heavy, repeated, muscular exercise, reductions in VO2, strongly suggestive of an increased fat oxidation, are not accompanied by a corresponding change in ventilation."}
{"id": "PMID:487270", "title": "The effect of spironolactone on the arterial water, cation, and norepinephrine contents of control and 'one-kidney-one-clip' hypertensive dogs.", "content": "Spironolactone at 10 mg per kilogram per day was given to control and 'one-kidney-one-clip' hypertensive dogs for 6--7 weeks. Compared with untreated animals the treated animals had a lower arterial water content, a higher arterial, myocardial, and skeletal muscle sodium content, and variable changes in tissue potassium concentrations.", "contents": "The effect of spironolactone on the arterial water, cation, and norepinephrine contents of control and 'one-kidney-one-clip' hypertensive dogs. Spironolactone at 10 mg per kilogram per day was given to control and 'one-kidney-one-clip' hypertensive dogs for 6--7 weeks. Compared with untreated animals the treated animals had a lower arterial water content, a higher arterial, myocardial, and skeletal muscle sodium content, and variable changes in tissue potassium concentrations."}
{"id": "PMID:487271", "title": "The effect of propranolol and dimethylpropranolol on cardiac conduction.", "content": "Isolated dog hearts perfused with blood from a donor dogand driven at two heart rates were used to compare the effects of propranolol with those of its quaternary ammonium derivative on atrial, atrioventricular (AV) nodal, and His-Purkinje conduction. Propranolol slowed only AV-nodal conduction, increasing the minimal conduction time and the effect of prematurity, without affecting fatigue. Practolol did not have this effect. Dimethylpropranolol had similar but not identical effects on the AV node, but also slowed atrial and ventricular conduction. In contrast with the quaternary derivative of lidocaine, dimethylpropranolol's effect on atrial and ventricular conduction was not dependent on the heart rate. The effect of dimethylpropranolol on ventricular conduction was observed at doses lower than those reported by others to be antiarrhythmic.", "contents": "The effect of propranolol and dimethylpropranolol on cardiac conduction. Isolated dog hearts perfused with blood from a donor dogand driven at two heart rates were used to compare the effects of propranolol with those of its quaternary ammonium derivative on atrial, atrioventricular (AV) nodal, and His-Purkinje conduction. Propranolol slowed only AV-nodal conduction, increasing the minimal conduction time and the effect of prematurity, without affecting fatigue. Practolol did not have this effect. Dimethylpropranolol had similar but not identical effects on the AV node, but also slowed atrial and ventricular conduction. In contrast with the quaternary derivative of lidocaine, dimethylpropranolol's effect on atrial and ventricular conduction was not dependent on the heart rate. The effect of dimethylpropranolol on ventricular conduction was observed at doses lower than those reported by others to be antiarrhythmic."}
{"id": "PMID:487272", "title": "Action of narcotic analgesics and antagonists on spinal units responding to natural stimulation in the cat.", "content": "Morphine and morphine-related agents were applied by microiontophoresis in the lumbar spinal cord of spinal cats to single units classified on the basis of their responses to natural cutaneous or proprioceptive stimulation. Opiate application had a current-dependent depressant effect on the ongoing activities of about one-third of the units tested. This effect was observed in laminae I and IV--VI, but only with units responding to noxious cutaneous stimuli: the nociceptive responses were themselves depressed. Excitatory and inhibitory responses to glutamate and gamma-aminobutyric acid, respectively, were also depressed. Intravenous administration of the opiates at doses reported to produce analgesia in the cat also depressed only units responding to noxious cutaneous stimuli, including their nociceptive responses. This depression could be reversed by either the iontophoretic application (100 nA) or the intravenous administration (0.1--0.8 mg/kg) of naloxone. These results are interpreted as further evidence that the analgesic effects of opiates are at least partly due to an action at the spinal level.", "contents": "Action of narcotic analgesics and antagonists on spinal units responding to natural stimulation in the cat. Morphine and morphine-related agents were applied by microiontophoresis in the lumbar spinal cord of spinal cats to single units classified on the basis of their responses to natural cutaneous or proprioceptive stimulation. Opiate application had a current-dependent depressant effect on the ongoing activities of about one-third of the units tested. This effect was observed in laminae I and IV--VI, but only with units responding to noxious cutaneous stimuli: the nociceptive responses were themselves depressed. Excitatory and inhibitory responses to glutamate and gamma-aminobutyric acid, respectively, were also depressed. Intravenous administration of the opiates at doses reported to produce analgesia in the cat also depressed only units responding to noxious cutaneous stimuli, including their nociceptive responses. This depression could be reversed by either the iontophoretic application (100 nA) or the intravenous administration (0.1--0.8 mg/kg) of naloxone. These results are interpreted as further evidence that the analgesic effects of opiates are at least partly due to an action at the spinal level."}
{"id": "PMID:487273", "title": "A novel method to separate the layers of the intestine.", "content": "By applying cellulose acetate paper to the lumenal and serosal surface of the intestine of rats, we have divided the intestine into three layers. Examining the layers histologically, we have shown that most of the villi are removed from the mucosal surface, ther serosa alone is removed from the serosal surface, and muscle layers. This appears to provide a rapid procedure for separation of the layers of the intestine for histological and biochemical studies.", "contents": "A novel method to separate the layers of the intestine. By applying cellulose acetate paper to the lumenal and serosal surface of the intestine of rats, we have divided the intestine into three layers. Examining the layers histologically, we have shown that most of the villi are removed from the mucosal surface, ther serosa alone is removed from the serosal surface, and muscle layers. This appears to provide a rapid procedure for separation of the layers of the intestine for histological and biochemical studies."}
{"id": "PMID:487275", "title": "Carotid sinus reflex and norepinephrine release following acute volume depletion in dogs.", "content": "Norepinephrine (NE) release and pressor response to sympathetic stimulation were studied in dogs under furosemide-induced acute volume depletion. The rise in blood pressure observed following carotid clamping proved similar before and after acute salt and water depletion in the first group of animals and NE rose comparably in these two conditions. Similar results were obtained in a second group of dogs that received an angiotensin II converting enzyme inhibitor (CEI). This study shows that contrary to isotonic saline loading, acute salt and water depletion cause a progressive increase in NE plasma levels. Moreover, these results clearly demonstrate that the decrease in sympatho--adrenergic response and the predominant role played by the renin--aniotensin system during chronic salt depletion are not observed in acute conditions.", "contents": "Carotid sinus reflex and norepinephrine release following acute volume depletion in dogs. Norepinephrine (NE) release and pressor response to sympathetic stimulation were studied in dogs under furosemide-induced acute volume depletion. The rise in blood pressure observed following carotid clamping proved similar before and after acute salt and water depletion in the first group of animals and NE rose comparably in these two conditions. Similar results were obtained in a second group of dogs that received an angiotensin II converting enzyme inhibitor (CEI). This study shows that contrary to isotonic saline loading, acute salt and water depletion cause a progressive increase in NE plasma levels. Moreover, these results clearly demonstrate that the decrease in sympatho--adrenergic response and the predominant role played by the renin--aniotensin system during chronic salt depletion are not observed in acute conditions."}
{"id": "PMID:487277", "title": "Defect in regulation of membrane transport of monosaccharides in dystrophic muscle.", "content": "The penetration of a nonmetabolized glucose analogue, 3--O-methyl-D-glucose, across the plasma membranes of tissues from dystrophic mice and cardiomyopathic (dystrophic) hamsters has been compared with that of normal controls. Under basal conditions the penetration of test sugar was similar in lens and diaphragm of normal and dystrophic 129/ReJ mice. Stimulation of sugar transport by 2,4-dinitrophenol did occur in normal but not in dystrophic diaphragm. A submaximal concentration of insulin had a more variable effect in dystrophic than in normal muscle while a supramaximal concentration of the hormone increased the uptake of the glucose analogue to an equal extent in the two tissues. In the BIO 14.6 strain of cardiomyopathic hamsters, uncoupling of oxidative phosphorylation did not increase sugar transport in extensor digitorum longus muscles, while the normal effect was observed in dystrophic soleus and in both these muscles of the random bred controls. The absence of an effect by a condition simulating anoxia suggests that in dystrophy, certain muscles are unable to accelerate the entry of glucose when this is required.", "contents": "Defect in regulation of membrane transport of monosaccharides in dystrophic muscle. The penetration of a nonmetabolized glucose analogue, 3--O-methyl-D-glucose, across the plasma membranes of tissues from dystrophic mice and cardiomyopathic (dystrophic) hamsters has been compared with that of normal controls. Under basal conditions the penetration of test sugar was similar in lens and diaphragm of normal and dystrophic 129/ReJ mice. Stimulation of sugar transport by 2,4-dinitrophenol did occur in normal but not in dystrophic diaphragm. A submaximal concentration of insulin had a more variable effect in dystrophic than in normal muscle while a supramaximal concentration of the hormone increased the uptake of the glucose analogue to an equal extent in the two tissues. In the BIO 14.6 strain of cardiomyopathic hamsters, uncoupling of oxidative phosphorylation did not increase sugar transport in extensor digitorum longus muscles, while the normal effect was observed in dystrophic soleus and in both these muscles of the random bred controls. The absence of an effect by a condition simulating anoxia suggests that in dystrophy, certain muscles are unable to accelerate the entry of glucose when this is required."}
{"id": "PMID:487279", "title": "Bile flow and electrolyte composition of bile associated with maximum bilirubin excretion in sheep.", "content": "Changes in the composition of bile accompanying the maximum biliary excretion (Emax) of bilirubin were investigated in sheep. Sheep fitted with chronic 'T-tubes' in the common bile duct were infused with taurocholate and bilirubin at various rates. Bile collected during both pre- and post-bilirubin steady-state periods was analyzed for the biliary concentration of electrolytes, bile salts, and bilirubin. Bilirubin Emax was 24.6 mumol/min while bile salt excretion during this period was 103 mumol/min. At Emax bilirubin entry into bile reached a concentration of 16.1 mumol/mL, increased the biliary concentration of sodium, did not change osmolarity of bile, and did not increase bile flow. The data suggest that bilirubin either interacts with mixed micelles in bile or forms molecular aggregates.", "contents": "Bile flow and electrolyte composition of bile associated with maximum bilirubin excretion in sheep. Changes in the composition of bile accompanying the maximum biliary excretion (Emax) of bilirubin were investigated in sheep. Sheep fitted with chronic 'T-tubes' in the common bile duct were infused with taurocholate and bilirubin at various rates. Bile collected during both pre- and post-bilirubin steady-state periods was analyzed for the biliary concentration of electrolytes, bile salts, and bilirubin. Bilirubin Emax was 24.6 mumol/min while bile salt excretion during this period was 103 mumol/min. At Emax bilirubin entry into bile reached a concentration of 16.1 mumol/mL, increased the biliary concentration of sodium, did not change osmolarity of bile, and did not increase bile flow. The data suggest that bilirubin either interacts with mixed micelles in bile or forms molecular aggregates."}
{"id": "PMID:487280", "title": "Adrenergic presynaptic receptors: examination of a hypothesis in guinea pig vas deferens.", "content": "The hypothesis was examined that phenoxybenzamine enhances both the overflow of noradrenaline and the mechanical response in guinea pig vas deferens by blockade of presynaptic inhibitory receptors located on adrenergic nerve terminals which serve a negative-feedback function. Preparations were stimulated with a constant small number of pulses but at three different frequencies (1, 5, and 15 Hz) and the relative effectiveness of phenoxybenzamine in enhancing overflow assessed. According to the presynaptic receptor hypothesis inhibition of transmitter output should increase with increasing frequency due to increased activation of receptor sites by endogenously released noradrenaline. The antagonist enhanced the overflow of tritium but did so to a similar extent at all three frequencies, regardless of the length of the interval between pulses. Similarly, no evidence for a greater sensitization of the mechanical response by phenoxybenzamine at the higher frequencies was obtained. The conditions of the present experiment were considered optimal for the operation of the negative-feedback system and the results indicate that the physiological relevance of such a system is questionable.", "contents": "Adrenergic presynaptic receptors: examination of a hypothesis in guinea pig vas deferens. The hypothesis was examined that phenoxybenzamine enhances both the overflow of noradrenaline and the mechanical response in guinea pig vas deferens by blockade of presynaptic inhibitory receptors located on adrenergic nerve terminals which serve a negative-feedback function. Preparations were stimulated with a constant small number of pulses but at three different frequencies (1, 5, and 15 Hz) and the relative effectiveness of phenoxybenzamine in enhancing overflow assessed. According to the presynaptic receptor hypothesis inhibition of transmitter output should increase with increasing frequency due to increased activation of receptor sites by endogenously released noradrenaline. The antagonist enhanced the overflow of tritium but did so to a similar extent at all three frequencies, regardless of the length of the interval between pulses. Similarly, no evidence for a greater sensitization of the mechanical response by phenoxybenzamine at the higher frequencies was obtained. The conditions of the present experiment were considered optimal for the operation of the negative-feedback system and the results indicate that the physiological relevance of such a system is questionable."}
{"id": "PMID:487281", "title": "Effects of noradrenaline and nicotinic acid on plasma free fatty acids and oxygen consumption in cold-adapted rats.", "content": "A 3-h noradrenaline (NA) infusion (1.5 microgram kg-1 min-1) produced a sustained enhanced oxygen consumption (O2 cons.) in cold-adapted rats. Plasma free fatty acid (FFA) levels were elevated by NA in control and in cold-adapted rats, but to lesser extent in cold-adapted rats; the increase was maintained at a plateau in both groups during the entire period of NA infusion. A 1-h nicotinic acid (Nic A) infusion (1.5 mg kg-1 min-1) added to the NA infusion inhibited the calorigenic response to NA in cold-adapted rats and reduced the elevated plasma FFA concentration in control and in cold-adapted rats to values below basal levels. However, when the Nic A infusion was stopped, the O2 cons. was increased again in cold-adapted rats by the uninterrupted NA infusion, without the simultaneous increase of the plasma FFA concentration; the plasma FFA concentration was maintained in cold-adapted rats below basal values and merely brought back to basal levels in control rats. From these results, it is suggested that plasma FFA are not an essential substrate to the calorigenic response to NA observed in cold-adapted rats, as 85% of the response can occur when the plasma FFA concentration is very low.", "contents": "Effects of noradrenaline and nicotinic acid on plasma free fatty acids and oxygen consumption in cold-adapted rats. A 3-h noradrenaline (NA) infusion (1.5 microgram kg-1 min-1) produced a sustained enhanced oxygen consumption (O2 cons.) in cold-adapted rats. Plasma free fatty acid (FFA) levels were elevated by NA in control and in cold-adapted rats, but to lesser extent in cold-adapted rats; the increase was maintained at a plateau in both groups during the entire period of NA infusion. A 1-h nicotinic acid (Nic A) infusion (1.5 mg kg-1 min-1) added to the NA infusion inhibited the calorigenic response to NA in cold-adapted rats and reduced the elevated plasma FFA concentration in control and in cold-adapted rats to values below basal levels. However, when the Nic A infusion was stopped, the O2 cons. was increased again in cold-adapted rats by the uninterrupted NA infusion, without the simultaneous increase of the plasma FFA concentration; the plasma FFA concentration was maintained in cold-adapted rats below basal values and merely brought back to basal levels in control rats. From these results, it is suggested that plasma FFA are not an essential substrate to the calorigenic response to NA observed in cold-adapted rats, as 85% of the response can occur when the plasma FFA concentration is very low."}
{"id": "PMID:487282", "title": "Effect of acute and chronic renal denervation on renal function after release of unilateral ureteral obstruction in the rat.", "content": "The role of the renal nerves in determining renal function after relief of 24-h unilateral ureteral obstruction (UUO) was studied using clearance techniques in anaesthetized rats. Acute renal denervation during the first 1--2 h after relief of UUO resulted in a significant increase in glomerular filtration rate (GFR), renal plasma flow (RPF), urine flow, and sodium and potassium excretion, changes which were not seen in the sham-denervated postobstructive kidney. Acute denervation of sham-operated normal kidneys caused a similar natriuresis and diuresis but with no change in GFR or RPF. Chronic renal denervation 4--5 days before UUO denervated postobstructive controls, while chronic denervation alone was associated with a significantly higher urine flow and sodium excretion rate from the denervated kidney. The effectiveness of renal denervation was confirmed by demonstrating marked depletion of tissue catecholamines in the denervated kidney. It was concluded that renal nerve activity plays a significant but not a major role in the functional changes present after relief of UUO. Chronic renal denervation did not protect against the functional effects of unilateral ureteral obstruction.", "contents": "Effect of acute and chronic renal denervation on renal function after release of unilateral ureteral obstruction in the rat. The role of the renal nerves in determining renal function after relief of 24-h unilateral ureteral obstruction (UUO) was studied using clearance techniques in anaesthetized rats. Acute renal denervation during the first 1--2 h after relief of UUO resulted in a significant increase in glomerular filtration rate (GFR), renal plasma flow (RPF), urine flow, and sodium and potassium excretion, changes which were not seen in the sham-denervated postobstructive kidney. Acute denervation of sham-operated normal kidneys caused a similar natriuresis and diuresis but with no change in GFR or RPF. Chronic renal denervation 4--5 days before UUO denervated postobstructive controls, while chronic denervation alone was associated with a significantly higher urine flow and sodium excretion rate from the denervated kidney. The effectiveness of renal denervation was confirmed by demonstrating marked depletion of tissue catecholamines in the denervated kidney. It was concluded that renal nerve activity plays a significant but not a major role in the functional changes present after relief of UUO. Chronic renal denervation did not protect against the functional effects of unilateral ureteral obstruction."}
{"id": "PMID:487283", "title": "Simultaneous measurement of cell loss and gastric potential difference in the rat: effects of short-term fasting.", "content": "These experiments utilized ex vivo gastric chamber preparations in both fed rats and rats fasted for 14--18 h. A new, simple technique is described for the quantification of cells in small volumes of fluid. The data indicate that exposure to solutions of 50 mM HCl was accompanied by greater cell loss in fasted vs. fed animals. The gastric potential differences of mucosae exposed to Ringer's mammalian saline, and subsequently to 50 mM HCl, were consistently at least 10 mV more negative in fasted animals.", "contents": "Simultaneous measurement of cell loss and gastric potential difference in the rat: effects of short-term fasting. These experiments utilized ex vivo gastric chamber preparations in both fed rats and rats fasted for 14--18 h. A new, simple technique is described for the quantification of cells in small volumes of fluid. The data indicate that exposure to solutions of 50 mM HCl was accompanied by greater cell loss in fasted vs. fed animals. The gastric potential differences of mucosae exposed to Ringer's mammalian saline, and subsequently to 50 mM HCl, were consistently at least 10 mV more negative in fasted animals."}
{"id": "PMID:487284", "title": "Acetylcholine does not stimulate the release of growth hormone from perifused rat adenohypophyses.", "content": "Acetylcholine (1 micromol/L--1 mmol/L) has been reported to stimulate growth hormone release from perifused bovine pituitary slices. We have carried out studies using the perifused pars distalis of the rat adenohypophysis to see whether a similar response is observed. Neither acetylcholine, acetylcholine with eserine, nor carbamylcholine, at concentrations of 10(-9), 10(-6), and 10(-3) M. stimulated the release of growth hormone. Thus we could not demonstrate a similar reponse to acetylcholine in the pars distalis of the rat adenohypophysis as reported in the bovine gland.", "contents": "Acetylcholine does not stimulate the release of growth hormone from perifused rat adenohypophyses. Acetylcholine (1 micromol/L--1 mmol/L) has been reported to stimulate growth hormone release from perifused bovine pituitary slices. We have carried out studies using the perifused pars distalis of the rat adenohypophysis to see whether a similar response is observed. Neither acetylcholine, acetylcholine with eserine, nor carbamylcholine, at concentrations of 10(-9), 10(-6), and 10(-3) M. stimulated the release of growth hormone. Thus we could not demonstrate a similar reponse to acetylcholine in the pars distalis of the rat adenohypophysis as reported in the bovine gland."}
{"id": "PMID:487285", "title": "Uptake of liposome-entrapped mannitol by diaphragm.", "content": "Tissue uptake of liposome-entrapped radioactive mannitol was examined in rats and mice after both intravenous and intraperitoneal injection. In accord with results from other laboratories, liver and spleen effectively accumulated liposomes. Diaphragm also took up significant amounts of label. In nearly all cases the radioactive content of perfused tissues was less than tissues which were not perfused but this was statistically significant in only a few comparisons.", "contents": "Uptake of liposome-entrapped mannitol by diaphragm. Tissue uptake of liposome-entrapped radioactive mannitol was examined in rats and mice after both intravenous and intraperitoneal injection. In accord with results from other laboratories, liver and spleen effectively accumulated liposomes. Diaphragm also took up significant amounts of label. In nearly all cases the radioactive content of perfused tissues was less than tissues which were not perfused but this was statistically significant in only a few comparisons."}
{"id": "PMID:487286", "title": "Isoniazid phenotyping of black as well as white patients.", "content": "The isoniazid phenotyping in black patients from Birmingham (Alabama) as well as from South Africa yielded a higher frequency of fast inactivation than that in the Canadian and U.S. white participants. Following an oral test dose of 10 mg isoniazid per kilogram, the incidence of fast acetylation was 58.7 and 60.3% in South African and Birmingham blacks, respectively. In the Canadian and Birmingham Caucasians the rate was 41.9 and 41.0%, respectively.", "contents": "Isoniazid phenotyping of black as well as white patients. The isoniazid phenotyping in black patients from Birmingham (Alabama) as well as from South Africa yielded a higher frequency of fast inactivation than that in the Canadian and U.S. white participants. Following an oral test dose of 10 mg isoniazid per kilogram, the incidence of fast acetylation was 58.7 and 60.3% in South African and Birmingham blacks, respectively. In the Canadian and Birmingham Caucasians the rate was 41.9 and 41.0%, respectively."}
{"id": "PMID:487287", "title": "Antagonists of angiotensin II containing N-methyl-L-alanine and DL-nipecotic acid in position 7.", "content": "[1-sarcosine, 7-N-methyl-L-alanine, 8-isoleucine]-Angiotensin II and [1-sarcosine, 7-DL-nipecotic acid, 8-isoleucine]-angiotensin II were synthesized by the solid-phase method and purified by cation-exchange chromatography and high-pressure liquid chromatography. In the isolated rat uterus these analogs and less than 0.1% of the myotropic activity of angiotensin II and inhibited angiotensin II with pA2 values of 8.2 and 7.8, respectively. In the rat pressor assay (vagotomized ganglion blocked rat) these analogs had 0.9 and 2.8%, respectively, of the pressor activity of angiotensin II. The results show that the proline residue in position 7 of [Sar1,Ile8]-angiotensin II may be replaced by other secondary amino acids without disrupting interactions at angiotensin II receptors.", "contents": "Antagonists of angiotensin II containing N-methyl-L-alanine and DL-nipecotic acid in position 7. [1-sarcosine, 7-N-methyl-L-alanine, 8-isoleucine]-Angiotensin II and [1-sarcosine, 7-DL-nipecotic acid, 8-isoleucine]-angiotensin II were synthesized by the solid-phase method and purified by cation-exchange chromatography and high-pressure liquid chromatography. In the isolated rat uterus these analogs and less than 0.1% of the myotropic activity of angiotensin II and inhibited angiotensin II with pA2 values of 8.2 and 7.8, respectively. In the rat pressor assay (vagotomized ganglion blocked rat) these analogs had 0.9 and 2.8%, respectively, of the pressor activity of angiotensin II. The results show that the proline residue in position 7 of [Sar1,Ile8]-angiotensin II may be replaced by other secondary amino acids without disrupting interactions at angiotensin II receptors."}
{"id": "PMID:487288", "title": "Correction factor for the estimation of plasma glucose synthesis from the transfer of 14C-atoms from labelled substrate in vivo: A preliminary report.", "content": "Based on a previously designed method for the estimation of the contribution of C-atoms by acetylcoenzyme A to the hepatic oxaloacetate pool, the loss of 14C-atoms on their way from a precursor to plasma glucose due to 'metabolic exchange' was estimated in normal and diabetic dogs and in normal rats. Due to this loss of 14C-atoms, the rates of gluconeogenesis when calculated from the transfer of 14C-atoms from precursors (other than glycerol) are underestimated by a factor of 2.2 +/- 0.07 in normal, 1.8 +/- 0.05 in diabetic dogs, and by 1.55 +/- 0.04 in normal rats.", "contents": "Correction factor for the estimation of plasma glucose synthesis from the transfer of 14C-atoms from labelled substrate in vivo: A preliminary report. Based on a previously designed method for the estimation of the contribution of C-atoms by acetylcoenzyme A to the hepatic oxaloacetate pool, the loss of 14C-atoms on their way from a precursor to plasma glucose due to 'metabolic exchange' was estimated in normal and diabetic dogs and in normal rats. Due to this loss of 14C-atoms, the rates of gluconeogenesis when calculated from the transfer of 14C-atoms from precursors (other than glycerol) are underestimated by a factor of 2.2 +/- 0.07 in normal, 1.8 +/- 0.05 in diabetic dogs, and by 1.55 +/- 0.04 in normal rats."}
{"id": "PMID:487291", "title": "Ecology and taxonomy of bacteria attaching to wood surfaces in a tropical harbor.", "content": "Water, sediment, and wooden pilings, samples of which were collected from a harbor in Puerto Rico during the course of a long-term study of biofouling of wood treated with creosote and related compounds, were found to support growth of microbial populations, the dominant taxa of which included Hyphomicrobium, Hyphomonas, Pseudomonas, Vibrio, and Bacillus. New wood exposed to the harbor water was rapidly colonized by Hyphomicrobium vulgare. Old pilings in an advanced stage of biodeterioration maintained a diverse bacterial microflora, representatives of which were also found widely distributed in the water column and sediment. Evidence for bacterial species succession was obtained, indicating that microbial interactions are important for attachment to, and subsequent colonization of, wood surfaces in the marine environment.", "contents": "Ecology and taxonomy of bacteria attaching to wood surfaces in a tropical harbor. Water, sediment, and wooden pilings, samples of which were collected from a harbor in Puerto Rico during the course of a long-term study of biofouling of wood treated with creosote and related compounds, were found to support growth of microbial populations, the dominant taxa of which included Hyphomicrobium, Hyphomonas, Pseudomonas, Vibrio, and Bacillus. New wood exposed to the harbor water was rapidly colonized by Hyphomicrobium vulgare. Old pilings in an advanced stage of biodeterioration maintained a diverse bacterial microflora, representatives of which were also found widely distributed in the water column and sediment. Evidence for bacterial species succession was obtained, indicating that microbial interactions are important for attachment to, and subsequent colonization of, wood surfaces in the marine environment."}
{"id": "PMID:487292", "title": "[Experimental study of vibrio parahaemolyticus (biotype 2) transfer from water and sediments to benthic marine food chain organisms].", "content": "Transfer of Vibrio parahaemolyticus (biotype 2) from sediments to water and from water to benthic marine organisms was studied experimentally using a streptomycin-resistant strain. Transmission by trophic pathways was also studied using reconstituted marine food chains (Mytilus edulis, Nereis diversicolor, Carcinus maenas, Scorpaena porcus, Mus musculus). Water colonization by sediments could be observed only at temperatures above 16 degrees C. Sediments could well constitute a disseminating reservoir for these germs, their cycle in water being dependent of the cycle followed in the sediments. Contamination of animal organisms is essentially effected by a direct mean, either water or sediments; transfer by trophic pathways being negligible. Infection of land consumers (mice) is linked quantitatively to the nature of the last marine organism of the food chain since bacteria can flourish in the digestive tract of certain animals (Carcinus maenas).", "contents": "[Experimental study of vibrio parahaemolyticus (biotype 2) transfer from water and sediments to benthic marine food chain organisms]. Transfer of Vibrio parahaemolyticus (biotype 2) from sediments to water and from water to benthic marine organisms was studied experimentally using a streptomycin-resistant strain. Transmission by trophic pathways was also studied using reconstituted marine food chains (Mytilus edulis, Nereis diversicolor, Carcinus maenas, Scorpaena porcus, Mus musculus). Water colonization by sediments could be observed only at temperatures above 16 degrees C. Sediments could well constitute a disseminating reservoir for these germs, their cycle in water being dependent of the cycle followed in the sediments. Contamination of animal organisms is essentially effected by a direct mean, either water or sediments; transfer by trophic pathways being negligible. Infection of land consumers (mice) is linked quantitatively to the nature of the last marine organism of the food chain since bacteria can flourish in the digestive tract of certain animals (Carcinus maenas)."}
{"id": "PMID:487293", "title": "Trypsin-uncoupled synthesis and secretion of yeast invertase: implications for the mechanism of secretion.", "content": "The subcellular distribution of invertase was examined after synthesis and secretion by sphaeroplasts had been uncoupled by the addition of 30 microgram mL-1 trypsin. Sphaeroplasts secreted only the high molecular weight invertase during uncoupling by trypsin. The level of low molecular weight, 'small' invertase in the soluble internal pool was found to be elevated by over fivefold, and the membrane-associated pool was found to contain low molecular weight invertase in addition to intermediate molecular weight invertase, after 1.5 h of trypsin treatment. Purified plasma membranes from trypsin-treated sphaeroplasts had no detectable mannan synthetase activity. On the basis of these and previous findings, a working hypothesis wherein invertase is synthesized on the internal surface of the plasma membrane and glycosylated during its transit to the external surface is presented.", "contents": "Trypsin-uncoupled synthesis and secretion of yeast invertase: implications for the mechanism of secretion. The subcellular distribution of invertase was examined after synthesis and secretion by sphaeroplasts had been uncoupled by the addition of 30 microgram mL-1 trypsin. Sphaeroplasts secreted only the high molecular weight invertase during uncoupling by trypsin. The level of low molecular weight, 'small' invertase in the soluble internal pool was found to be elevated by over fivefold, and the membrane-associated pool was found to contain low molecular weight invertase in addition to intermediate molecular weight invertase, after 1.5 h of trypsin treatment. Purified plasma membranes from trypsin-treated sphaeroplasts had no detectable mannan synthetase activity. On the basis of these and previous findings, a working hypothesis wherein invertase is synthesized on the internal surface of the plasma membrane and glycosylated during its transit to the external surface is presented."}
{"id": "PMID:487294", "title": "Aquarium pets as a source of antibiotic-resistant salmonellae.", "content": "Thirteen serotypes of Salmonella isolated from imported ornamental aquarium frogs, snails, and their waters were shown to be multi-drug-resistant. Among the resistances exhibited were resistance to gentamicin, sulfamethoxazole-trimethoprim, cephalothin, and nalidixic acid. Frog isolates displayed eight different patterns and snails isolates had two different resistance patterns. The most common serotype, Salmonella typhimurium, was resistant to 18 antibacterials while other salmonellae were resistant to 9 to 16 antibacterials. Resistances in S. typhimurium and S. bovis-morbificans were conjugative and a number of R plasmids participated in the resistance. The plasmid-mediated resistance in S. typhimurium was stable and the levels of resistance conferred were markedly higher than in the other salmonellae tested. Resistance of other serotypes was non-conjugative and resistance to the beta-lactam antibiotics was unstable.", "contents": "Aquarium pets as a source of antibiotic-resistant salmonellae. Thirteen serotypes of Salmonella isolated from imported ornamental aquarium frogs, snails, and their waters were shown to be multi-drug-resistant. Among the resistances exhibited were resistance to gentamicin, sulfamethoxazole-trimethoprim, cephalothin, and nalidixic acid. Frog isolates displayed eight different patterns and snails isolates had two different resistance patterns. The most common serotype, Salmonella typhimurium, was resistant to 18 antibacterials while other salmonellae were resistant to 9 to 16 antibacterials. Resistances in S. typhimurium and S. bovis-morbificans were conjugative and a number of R plasmids participated in the resistance. The plasmid-mediated resistance in S. typhimurium was stable and the levels of resistance conferred were markedly higher than in the other salmonellae tested. Resistance of other serotypes was non-conjugative and resistance to the beta-lactam antibiotics was unstable."}
{"id": "PMID:487295", "title": "Evidence for chemotaxis during sexual development in Dictyostelium discoideum.", "content": "Amoebae in mated cultures of Dictyostelium discoideum show oriented movement towards young aggregates, suggesting that cemotaxis is involved in macrocyst development. Amoebae also show directional movement towards midendocyte stages, indicating that as the macrocyst develops it continues to be a source of chemoattractant. These data are discussed in terms of our current knowledge about mating in the cellular slime moulds.", "contents": "Evidence for chemotaxis during sexual development in Dictyostelium discoideum. Amoebae in mated cultures of Dictyostelium discoideum show oriented movement towards young aggregates, suggesting that cemotaxis is involved in macrocyst development. Amoebae also show directional movement towards midendocyte stages, indicating that as the macrocyst develops it continues to be a source of chemoattractant. These data are discussed in terms of our current knowledge about mating in the cellular slime moulds."}
{"id": "PMID:487296", "title": "The effects of partial chronic denervation on forearm metabolism.", "content": "Effects of chronic denervation upon in vivo forearm metabolism were studied in six patients and six controls. The diagnosis was amyotrophic lateral sclerosis in four patients, the neuronal form of Charcot-Marie Tooth disease in one patient, and an unclassified chronic disease of the lower motor neurons in one patient. In all cases the forearm muscles showed clinical weakness and electrical evidence of denervation, while muscle biopsy from a proximal muscle of the upper limb showed typical denervation atrophy. At rest there was increased oxygen utilization and lactate output as well as a tendency for increased uptake of glucose and long chain fatty acids from arterial blood per 100 ml of forearm tissue. During exercise the abnormally high lactate output increased further. An increased arterial lactate concentration was present during rest and exercise. Oxidation of fatty acids was not impaired. It is suggested that these abnormalities are consistent with an augmented utilization of blood borne fuels at rest by denervated muscles. A concurrent regional ischemia of muscles during rest and exercise, possibly due to defective autoregulation of skeletal muscle blood flow, may explain the abnormally high lactate generation.", "contents": "The effects of partial chronic denervation on forearm metabolism. Effects of chronic denervation upon in vivo forearm metabolism were studied in six patients and six controls. The diagnosis was amyotrophic lateral sclerosis in four patients, the neuronal form of Charcot-Marie Tooth disease in one patient, and an unclassified chronic disease of the lower motor neurons in one patient. In all cases the forearm muscles showed clinical weakness and electrical evidence of denervation, while muscle biopsy from a proximal muscle of the upper limb showed typical denervation atrophy. At rest there was increased oxygen utilization and lactate output as well as a tendency for increased uptake of glucose and long chain fatty acids from arterial blood per 100 ml of forearm tissue. During exercise the abnormally high lactate output increased further. An increased arterial lactate concentration was present during rest and exercise. Oxidation of fatty acids was not impaired. It is suggested that these abnormalities are consistent with an augmented utilization of blood borne fuels at rest by denervated muscles. A concurrent regional ischemia of muscles during rest and exercise, possibly due to defective autoregulation of skeletal muscle blood flow, may explain the abnormally high lactate generation."}
{"id": "PMID:487298", "title": "Cerebellar form of progressive multifocal leukoencephalopathy (PML).", "content": "A case of progressive multifocal leukoencephalopathy (PML) with selective involvement of cerebellum and brain stem is compared with nine other published cases. Recognition of PML presenting with ataxia before cerebral manifestations is stressed especially, since promising results with some antiviral drugs have been claimed.", "contents": "Cerebellar form of progressive multifocal leukoencephalopathy (PML). A case of progressive multifocal leukoencephalopathy (PML) with selective involvement of cerebellum and brain stem is compared with nine other published cases. Recognition of PML presenting with ataxia before cerebral manifestations is stressed especially, since promising results with some antiviral drugs have been claimed."}
{"id": "PMID:487299", "title": "[Criteria of the diagnosis and treatment of carotid atheromatosis].", "content": "This retrospective study concerning 102 patients was undertaken to study the therapeutic decisions in these patients when they presented with symptoms of cerebral vascular insufficiency, mostly from the territory of the carotid artery, either transient or accompanied by a deficit. The current literature on the subject is also mentioned. The clinical outcome with regard to the quality of life, seems to have been better in patients who were subjected to carotid endarterectomies than in those who received medical treatment.", "contents": "[Criteria of the diagnosis and treatment of carotid atheromatosis]. This retrospective study concerning 102 patients was undertaken to study the therapeutic decisions in these patients when they presented with symptoms of cerebral vascular insufficiency, mostly from the territory of the carotid artery, either transient or accompanied by a deficit. The current literature on the subject is also mentioned. The clinical outcome with regard to the quality of life, seems to have been better in patients who were subjected to carotid endarterectomies than in those who received medical treatment."}
{"id": "PMID:487300", "title": "Melanin containing neurofibroma: case report with evidence of Schwann cell origin of melanin.", "content": "This case report describes a melanin-containing neurofibroma involving a spinal nerve root. Electron microscopy of the tumor shows that neoplastic Schwann cells are capable of melanogenesis. Although this capability is suggested in the literature, few reports provide ultrastructural confirmation. The likely identity between reported \"cellular blue nevi of spinal nerve roots\" and nerve sheath tumors is discussed. It is possible that pigmented nerve sheath tumors behave more aggressively than nonpigmented ones, although it is debatable.", "contents": "Melanin containing neurofibroma: case report with evidence of Schwann cell origin of melanin. This case report describes a melanin-containing neurofibroma involving a spinal nerve root. Electron microscopy of the tumor shows that neoplastic Schwann cells are capable of melanogenesis. Although this capability is suggested in the literature, few reports provide ultrastructural confirmation. The likely identity between reported \"cellular blue nevi of spinal nerve roots\" and nerve sheath tumors is discussed. It is possible that pigmented nerve sheath tumors behave more aggressively than nonpigmented ones, although it is debatable."}
{"id": "PMID:487302", "title": "Cardiovascular evaluation of obligate heterozygotes in Friedreich's ataxia.", "content": "A study of 17 parents (obligate heterozygotes) of children with Friedreich's ataxia was carried out. In addition to medical histories and physical examinations, a standard 12 lead ECG tracing was obtained. In the age group below 50, there was no significant evidence of ischaemic or primary cardiomyopathy. Older subjects had more frequent risk factors for arteriosclerotic heart disease.", "contents": "Cardiovascular evaluation of obligate heterozygotes in Friedreich's ataxia. A study of 17 parents (obligate heterozygotes) of children with Friedreich's ataxia was carried out. In addition to medical histories and physical examinations, a standard 12 lead ECG tracing was obtained. In the age group below 50, there was no significant evidence of ischaemic or primary cardiomyopathy. Older subjects had more frequent risk factors for arteriosclerotic heart disease."}
{"id": "PMID:487303", "title": "Evolution of cardio-pulmonary involvement in Friedreich's ataxia.", "content": "The evolution of 15 patients initially evaluated during Phase One of the Quebec Cooperative Study of Friedreich's ataxia has been studied approximately three years later. It is concluded that the deterioration of cardio-pulmonary function in Friedreich's ataxia is multifactorial. The neuromyopathy (or the underlying metabolic or cellular defect) appears to be the main contributing factor to the deterioration of cardio-pulmonary function, which is exacerbated by the scoliosis and varying severity of the cardiomyopathy.", "contents": "Evolution of cardio-pulmonary involvement in Friedreich's ataxia. The evolution of 15 patients initially evaluated during Phase One of the Quebec Cooperative Study of Friedreich's ataxia has been studied approximately three years later. It is concluded that the deterioration of cardio-pulmonary function in Friedreich's ataxia is multifactorial. The neuromyopathy (or the underlying metabolic or cellular defect) appears to be the main contributing factor to the deterioration of cardio-pulmonary function, which is exacerbated by the scoliosis and varying severity of the cardiomyopathy."}
{"id": "PMID:487304", "title": "Regulation of respiration in Friedreich's ataxia.", "content": "Friedreich's Ataxia (F.A.) is a degenerative disease which commonly leads to premature death of cardiorespiratory origin. To explain the early death of these patients, previous investigations have established the existence of 1) a cardiomyopathy in nearly 100% of cases, 2) a restrictive pulmonary syndrome of scoliotic origin and 3) a mild hypoxemia associated with slight respiratory alkalosis and a normal oxyhemoglobin dissociation curve. To further assess the cause of early death in patients with such neuromyopathy, we evaluated, in eleven F.A. patients, the sensitivity of the respiratory centers to hypercapnia, hypoxia, and hyperoxia. Ventilatory (VE, VT, F, VT/Ti) and occlusion pressure (P0.1) responses were taken as indices of the respiratory centers output during progressive hypercapnia (Read's method) and isocarbic hypoxia (Weil's method). We studied 11 Friedreich's Ataxia patients and 11 age, sex, and armspan matched controls. The responses of patients to hypercapnia were significantly greater than controls but their responses to hypoxia were similar to controls. Our study establishes that the respiratory centers are functioning adequately in early Friedreich's Ataxia and do not contribute to cardio-respiratory insufficiency in such neuromyopathy.", "contents": "Regulation of respiration in Friedreich's ataxia. Friedreich's Ataxia (F.A.) is a degenerative disease which commonly leads to premature death of cardiorespiratory origin. To explain the early death of these patients, previous investigations have established the existence of 1) a cardiomyopathy in nearly 100% of cases, 2) a restrictive pulmonary syndrome of scoliotic origin and 3) a mild hypoxemia associated with slight respiratory alkalosis and a normal oxyhemoglobin dissociation curve. To further assess the cause of early death in patients with such neuromyopathy, we evaluated, in eleven F.A. patients, the sensitivity of the respiratory centers to hypercapnia, hypoxia, and hyperoxia. Ventilatory (VE, VT, F, VT/Ti) and occlusion pressure (P0.1) responses were taken as indices of the respiratory centers output during progressive hypercapnia (Read's method) and isocarbic hypoxia (Weil's method). We studied 11 Friedreich's Ataxia patients and 11 age, sex, and armspan matched controls. The responses of patients to hypercapnia were significantly greater than controls but their responses to hypoxia were similar to controls. Our study establishes that the respiratory centers are functioning adequately in early Friedreich's Ataxia and do not contribute to cardio-respiratory insufficiency in such neuromyopathy."}
{"id": "PMID:487305", "title": "Oculomotor abnormalities in Friedreich's ataxia.", "content": "A clinical neuro-ophthalmological and electro-oculographic study was made on fourteen patients with Friedreich's ataxia. None had evidence of optic nerve dysfunction. No patient complained of oscillopsia although all had ocular motor deficits of varying degrees, which appeared to be related to the severity of the general manifestations of the disease. The defects comprised square wave jerks, jerky pursuit with inability to maintain eccentric gaze resulting in gaze paretic nystagmus and rebound nystagmus. There was failure to suppress by fixation the vestibulo-ocular reflex. The slow phase velocity of caloric nystagmus was always of reduced velocity. There was inability to augment the slow phase velocity of optokinetic nystagmus with increasing stimulus velocity. Abnormalities of the saccadic system were manifest particularly as hypermetria. These signs in combination are suggestive of disease involving the cerebellar flocculus and vermis or their brain stem connections. No abnormalities were found in 17 parents or siblings.", "contents": "Oculomotor abnormalities in Friedreich's ataxia. A clinical neuro-ophthalmological and electro-oculographic study was made on fourteen patients with Friedreich's ataxia. None had evidence of optic nerve dysfunction. No patient complained of oscillopsia although all had ocular motor deficits of varying degrees, which appeared to be related to the severity of the general manifestations of the disease. The defects comprised square wave jerks, jerky pursuit with inability to maintain eccentric gaze resulting in gaze paretic nystagmus and rebound nystagmus. There was failure to suppress by fixation the vestibulo-ocular reflex. The slow phase velocity of caloric nystagmus was always of reduced velocity. There was inability to augment the slow phase velocity of optokinetic nystagmus with increasing stimulus velocity. Abnormalities of the saccadic system were manifest particularly as hypermetria. These signs in combination are suggestive of disease involving the cerebellar flocculus and vermis or their brain stem connections. No abnormalities were found in 17 parents or siblings."}
{"id": "PMID:487306", "title": "Brain lesions in Friedreich's ataxia.", "content": "An estimate is given of the frequency of pathological changes (assessed by cell populations and from myelin staining) in the brains of 15 cases of Friedreich's ataxia. Mention is made of the special problem of distinguishing primary degenerative lesions from those due to circulatory disturbances arising from the patients cardiac disease.", "contents": "Brain lesions in Friedreich's ataxia. An estimate is given of the frequency of pathological changes (assessed by cell populations and from myelin staining) in the brains of 15 cases of Friedreich's ataxia. Mention is made of the special problem of distinguishing primary degenerative lesions from those due to circulatory disturbances arising from the patients cardiac disease."}
{"id": "PMID:487307", "title": "Oculomotor and vestibular findings in autosomal recessive spastic ataxia of Charlevoix-Saguenay.", "content": "Electronystagmographic recordings were made of oculomotor and vestibular function in 11 patients with autosomal recessive spastic ataxia of Charlevoix-Saguenay. All had horizontal gaze nystagmus, marked impairment of smooth ocular pursuit and optokinetic nystagmus, and defective fixation suppression of caloric nystagmus. Many had saccadic dysmetria, but saccade velocity was probably unaffected. Abnormalities pointing to brainstem disturbance were sparse. The findings are thought to indicate mainly diffuse cerebellar disease, with particular involvement of vermis and vestibulo-cerebellum.", "contents": "Oculomotor and vestibular findings in autosomal recessive spastic ataxia of Charlevoix-Saguenay. Electronystagmographic recordings were made of oculomotor and vestibular function in 11 patients with autosomal recessive spastic ataxia of Charlevoix-Saguenay. All had horizontal gaze nystagmus, marked impairment of smooth ocular pursuit and optokinetic nystagmus, and defective fixation suppression of caloric nystagmus. Many had saccadic dysmetria, but saccade velocity was probably unaffected. Abnormalities pointing to brainstem disturbance were sparse. The findings are thought to indicate mainly diffuse cerebellar disease, with particular involvement of vermis and vestibulo-cerebellum."}
{"id": "PMID:487308", "title": "Electromyography and nerve conduction studies in Friedreich's ataxia and autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS).", "content": "Twenty four ataxic patients were investigated with electromyography and nerve conduction studies. They were divided in two groups according to the area they came from, the evolution of the disease, and the clinical signs. Group I patients from the Rimouski area displayed all the clinical and electrophysiological signs of Friedreich's ataxia. Group II comprised patients who presented with a new syndrome known as the autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). Although the clinical evolution was better in the latter, there were more electromyographic signs of denervation and the motor conduction velocities were slower. Both groups showed identical and important abnormalities in sensory nerve conduction. The results of electrophysiological studies in spastic ataxia have not been reported to our knowledge. They underline the place of spastic ataxia as distinct from Friedreich's ataxia, spastic paraplegia, and the known familial neuropathies.", "contents": "Electromyography and nerve conduction studies in Friedreich's ataxia and autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). Twenty four ataxic patients were investigated with electromyography and nerve conduction studies. They were divided in two groups according to the area they came from, the evolution of the disease, and the clinical signs. Group I patients from the Rimouski area displayed all the clinical and electrophysiological signs of Friedreich's ataxia. Group II comprised patients who presented with a new syndrome known as the autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). Although the clinical evolution was better in the latter, there were more electromyographic signs of denervation and the motor conduction velocities were slower. Both groups showed identical and important abnormalities in sensory nerve conduction. The results of electrophysiological studies in spastic ataxia have not been reported to our knowledge. They underline the place of spastic ataxia as distinct from Friedreich's ataxia, spastic paraplegia, and the known familial neuropathies."}
{"id": "PMID:487309", "title": "Electroencephalographic findings in Friedreich's ataxia and autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS).", "content": "Electroencephalographic studies have been done in two groups of hereditary ataxia: a group bearing the classical features of Friedreich's ataxia and a group clinically different described as autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). The qualitative anomalies observed in the two groups were similar and were comparable with the data reported in the literature. However, the main difference between the two groups is the greater incidence of EEG abnormalities in the ARSACS group, which suggests more involvement of the cortical and subcortical structures. This is reinforced by the lower I.Q. performance in the latter patients. Some comments are made about focal EEG findings, behavior and I.Q. In general, EEG was not considered a valuable instrument for diagnosis since no qualitative electric pattern could be identified. With regard to prognosis, EEG cannot be used as a criterion, since there is no relation between the degree of anomalies and the severity of the disease and since EEG does not worsen with the progression of the disease.", "contents": "Electroencephalographic findings in Friedreich's ataxia and autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). Electroencephalographic studies have been done in two groups of hereditary ataxia: a group bearing the classical features of Friedreich's ataxia and a group clinically different described as autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). The qualitative anomalies observed in the two groups were similar and were comparable with the data reported in the literature. However, the main difference between the two groups is the greater incidence of EEG abnormalities in the ARSACS group, which suggests more involvement of the cortical and subcortical structures. This is reinforced by the lower I.Q. performance in the latter patients. Some comments are made about focal EEG findings, behavior and I.Q. In general, EEG was not considered a valuable instrument for diagnosis since no qualitative electric pattern could be identified. With regard to prognosis, EEG cannot be used as a criterion, since there is no relation between the degree of anomalies and the severity of the disease and since EEG does not worsen with the progression of the disease."}
{"id": "PMID:487310", "title": "Computed tomography of posterior fossa in hereditary ataxias.", "content": "Nine cases of Friedreich's ataxia and seven cases of autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) were submitted to neuroradiological procedures to determine the extent of atrophic processes in the central nervous system. All cases had a computerized cerebral tomography and five were studied with pneumoencephalography. The results show a correlation between the two tests and the comparison between Friedreich's ataxia and ARSACS. In Friedreich's ataxia, the radiological signs are variable and discrete in most of the cases. In ARSACS there are constant signs of cerebellar atrophy almost limited to the superior parts of the vermis and anterior lobes.", "contents": "Computed tomography of posterior fossa in hereditary ataxias. Nine cases of Friedreich's ataxia and seven cases of autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) were submitted to neuroradiological procedures to determine the extent of atrophic processes in the central nervous system. All cases had a computerized cerebral tomography and five were studied with pneumoencephalography. The results show a correlation between the two tests and the comparison between Friedreich's ataxia and ARSACS. In Friedreich's ataxia, the radiological signs are variable and discrete in most of the cases. In ARSACS there are constant signs of cerebellar atrophy almost limited to the superior parts of the vermis and anterior lobes."}
{"id": "PMID:487311", "title": "The neuropathy of Charlevoix-Saguenay ataxia: an electrophysiological and pathological study.", "content": "Two female patients aged 30 and 40 years with the Charlevoix-Saguenay ataxia were studied. Both had absent sensory action potentials in upper and lower extremities but, unlike typical cases of Friedreich's ataxia, they displayed a marked slowing of motor conduction velocities. Sural nerve biopsies taken from calf and ankle revealed a severe loss of large myelinated axons contrasting with a normal myelinated fiber density. Evidence for active axonal degeneration was scarce, with no indication of axonal regeneration. Teased myelinated fibers revealed an increased variability of internodal length but no evidence for myelin breakdown. These findings support, as a primary defect, a developmental abnormality of peripheral nerve, namely a lack of maturation of large myelinated axons and possibly a faulty myelination of nerve fibers. We think it is unlikely to represent a progressive axonal atrophic or dystrophic process, as suggested in Friedreich's ataxia.", "contents": "The neuropathy of Charlevoix-Saguenay ataxia: an electrophysiological and pathological study. Two female patients aged 30 and 40 years with the Charlevoix-Saguenay ataxia were studied. Both had absent sensory action potentials in upper and lower extremities but, unlike typical cases of Friedreich's ataxia, they displayed a marked slowing of motor conduction velocities. Sural nerve biopsies taken from calf and ankle revealed a severe loss of large myelinated axons contrasting with a normal myelinated fiber density. Evidence for active axonal degeneration was scarce, with no indication of axonal regeneration. Teased myelinated fibers revealed an increased variability of internodal length but no evidence for myelin breakdown. These findings support, as a primary defect, a developmental abnormality of peripheral nerve, namely a lack of maturation of large myelinated axons and possibly a faulty myelination of nerve fibers. We think it is unlikely to represent a progressive axonal atrophic or dystrophic process, as suggested in Friedreich's ataxia."}
{"id": "PMID:487312", "title": "A cluster of Friedreich's ataxia in Rimouski, Qu\u00e9bec.", "content": "We described a cluster of 8 independent sibships of Friedreich's ataxia in the St-Fabien parish of Rimouski and have shown that they are all related within 6 generations. The study of this geographic and genetic isolate permitted the investigation of certain unusual features of the disease such as constant myopia, delayed reaction times to pain, flexor spasms, and a rapid evolution.", "contents": "A cluster of Friedreich's ataxia in Rimouski, Qu\u00e9bec. We described a cluster of 8 independent sibships of Friedreich's ataxia in the St-Fabien parish of Rimouski and have shown that they are all related within 6 generations. The study of this geographic and genetic isolate permitted the investigation of certain unusual features of the disease such as constant myopia, delayed reaction times to pain, flexor spasms, and a rapid evolution."}
{"id": "PMID:487313", "title": "Measurement of ataxia and related neurological signs in the laboratory rat.", "content": "The purpose of the present study was to design a standard battery of tests capable of quantitatively characterizing ataxia and concomitant neurological signs in the rat. In addition to a systematic analysis of the walking gait of animals, tests for activity, catalepsy, rigidity, and various reflexive responses were included in the battery. The standardization and validation of the test system was performed by determining and comparing profiles of neurobehavioral effects produced by 3-acetyl pyridine, acrylamide, pyrithiamine, and thiamine deficiency, four experimental treatments reported to induce ataxia in animals. Results indicate that profiles of neurobehavioral disturbances accompanying ataxia in animals varied distinctively with each experimental treatment.", "contents": "Measurement of ataxia and related neurological signs in the laboratory rat. The purpose of the present study was to design a standard battery of tests capable of quantitatively characterizing ataxia and concomitant neurological signs in the rat. In addition to a systematic analysis of the walking gait of animals, tests for activity, catalepsy, rigidity, and various reflexive responses were included in the battery. The standardization and validation of the test system was performed by determining and comparing profiles of neurobehavioral effects produced by 3-acetyl pyridine, acrylamide, pyrithiamine, and thiamine deficiency, four experimental treatments reported to induce ataxia in animals. Results indicate that profiles of neurobehavioral disturbances accompanying ataxia in animals varied distinctively with each experimental treatment."}
{"id": "PMID:487314", "title": "Amino acid changes in thiamine-deficient encephalopathy: some implications for the pathogenesis of Friedreich's ataxia.", "content": "Thiamine-deficient encephalopathy in the rat is characterized by ataxic gait, loss of righting reflex and curvature of the spine. Neurochemical changes include a diminished activity of cerebral pyruvate decarboxylase leading to abnormal pyruvate oxidation. The present study shows that this defective pyruvate oxidation produces a significant depletion of three important amino acid neurotransmitters, namely gamma aminobutyric acid (GABA), glutamic acid, and aspartic acid. Such changes could lead to severe neuronal dysfunction and the observed neurological symptoms of thiamine deficiency. Some implications for the pathogenesis of Friedreich's ataxia are discussed.", "contents": "Amino acid changes in thiamine-deficient encephalopathy: some implications for the pathogenesis of Friedreich's ataxia. Thiamine-deficient encephalopathy in the rat is characterized by ataxic gait, loss of righting reflex and curvature of the spine. Neurochemical changes include a diminished activity of cerebral pyruvate decarboxylase leading to abnormal pyruvate oxidation. The present study shows that this defective pyruvate oxidation produces a significant depletion of three important amino acid neurotransmitters, namely gamma aminobutyric acid (GABA), glutamic acid, and aspartic acid. Such changes could lead to severe neuronal dysfunction and the observed neurological symptoms of thiamine deficiency. Some implications for the pathogenesis of Friedreich's ataxia are discussed."}
{"id": "PMID:487315", "title": "Erythrocyte protoporphyrin levels in patients with Friedreich's and other ataxias.", "content": "Of 13 patients with Friedreich's ataxia (Type Ia) and 17 with type IIa recessive ataxias, all were found to have levels of \"free erythrocyte protoporphyrin\" (FEP) above the normal range. The rise in FEP in Friedreich's ataxia correlated well with the age of the individual and thus appears to be related to the course of the disease. Subjects with olivo-ponto-cerebellar atrophy, Charlevoix syndrome, Duchenne muscular dystrophy, and Parkinson's disease were also found to have significantly elevated FEP, although the distribution overlapped with the normal range. The finding of elevated FEP may indicate a relative heme deficiency in ataxia due to inhibition of ferrochelatase leading to a state of ineffective, persistent erythropoiesis. The possibility of a prostaglandin abnormality being related to this defect and to the pathogenesis of ataxia is considered.", "contents": "Erythrocyte protoporphyrin levels in patients with Friedreich's and other ataxias. Of 13 patients with Friedreich's ataxia (Type Ia) and 17 with type IIa recessive ataxias, all were found to have levels of \"free erythrocyte protoporphyrin\" (FEP) above the normal range. The rise in FEP in Friedreich's ataxia correlated well with the age of the individual and thus appears to be related to the course of the disease. Subjects with olivo-ponto-cerebellar atrophy, Charlevoix syndrome, Duchenne muscular dystrophy, and Parkinson's disease were also found to have significantly elevated FEP, although the distribution overlapped with the normal range. The finding of elevated FEP may indicate a relative heme deficiency in ataxia due to inhibition of ferrochelatase leading to a state of ineffective, persistent erythropoiesis. The possibility of a prostaglandin abnormality being related to this defect and to the pathogenesis of ataxia is considered."}
{"id": "PMID:487316", "title": "Glucose tolerance and erythrocyte insulin receptors in Friedreich's ataxia.", "content": "Detailed in vivo and in vitro studies of glucose and insulin metabolism in Friedreich's ataxia patients and unaffected family members have further defined the extent of the abnormalities in carbohydrate metabolism. The high incidence of glucose intolerance and a hyperinsulinemic response to a glucose challenge in a high percentage of Friedreich's ataxia patients has been confirmed. An increased incidence of glucose intolerance among heterozygotes is suggested, while the siblings show a more normal distribution of diabetes and a nearly normal insulin response to the glucose tolerance test. Human growth hormone patterns are normal for all groups. Preliminary studies of insulin binding to erythrocytes suggest a difference in the binding characteristics among diabetic Friedreich's ataxia patients, while the binding in the non-diabetic Friedreich's ataxia group is similar to that of non-diabetic controls. Results from a small group of non-diabetic siblings suggest a normal insulin binding, while a tendency toward increased binding at low insulin concentrations among diabetic family members is noted.", "contents": "Glucose tolerance and erythrocyte insulin receptors in Friedreich's ataxia. Detailed in vivo and in vitro studies of glucose and insulin metabolism in Friedreich's ataxia patients and unaffected family members have further defined the extent of the abnormalities in carbohydrate metabolism. The high incidence of glucose intolerance and a hyperinsulinemic response to a glucose challenge in a high percentage of Friedreich's ataxia patients has been confirmed. An increased incidence of glucose intolerance among heterozygotes is suggested, while the siblings show a more normal distribution of diabetes and a nearly normal insulin response to the glucose tolerance test. Human growth hormone patterns are normal for all groups. Preliminary studies of insulin binding to erythrocytes suggest a difference in the binding characteristics among diabetic Friedreich's ataxia patients, while the binding in the non-diabetic Friedreich's ataxia group is similar to that of non-diabetic controls. Results from a small group of non-diabetic siblings suggest a normal insulin binding, while a tendency toward increased binding at low insulin concentrations among diabetic family members is noted."}
{"id": "PMID:487317", "title": "Pyruvate dehydrogenase, lipoamide dehydrogenase and citrate synthase activity in fibroblasts from patients with Friedreich's and Charlevoix-Saguenay ataxia.", "content": "The activity of lipoamide dehydrogenase and two closely related enzymes was studied simultaneously in early, mild, and late passage fibroblast cultures. Friedreich's ataxia fibroblasts tended to lose pyruvate dehydrogenase and citrate synthase activities, while lipoamide dehydrogenase activity remained constant with aging of the cells. Mean pyruvate dehydrogenase activity was lower over-all in fibroblasts from ataxics. Mean citrate synthase activity was higher in ataxic fibroblasts. Present tissue culture media do not represent the best conditions in which to reproduce cofactor binding defects such as those found in other genetic diseases with structural enzyme mutations.", "contents": "Pyruvate dehydrogenase, lipoamide dehydrogenase and citrate synthase activity in fibroblasts from patients with Friedreich's and Charlevoix-Saguenay ataxia. The activity of lipoamide dehydrogenase and two closely related enzymes was studied simultaneously in early, mild, and late passage fibroblast cultures. Friedreich's ataxia fibroblasts tended to lose pyruvate dehydrogenase and citrate synthase activities, while lipoamide dehydrogenase activity remained constant with aging of the cells. Mean pyruvate dehydrogenase activity was lower over-all in fibroblasts from ataxics. Mean citrate synthase activity was higher in ataxic fibroblasts. Present tissue culture media do not represent the best conditions in which to reproduce cofactor binding defects such as those found in other genetic diseases with structural enzyme mutations."}
{"id": "PMID:487318", "title": "Pre- and postsynaptic effects of taurine and GABA in the cockroach central nervous system.", "content": "Taurine resembles GABA in its synaptic effects in the cockroach cercal nerve giant fiber synapse where it exerts a depressant action upon synaptic transmission. Both taurine and GABA produce an increased conductance of pre- and postsynaptic membranes through changes in the permeability of chloride ions.", "contents": "Pre- and postsynaptic effects of taurine and GABA in the cockroach central nervous system. Taurine resembles GABA in its synaptic effects in the cockroach cercal nerve giant fiber synapse where it exerts a depressant action upon synaptic transmission. Both taurine and GABA produce an increased conductance of pre- and postsynaptic membranes through changes in the permeability of chloride ions."}
{"id": "PMID:487319", "title": "Regional distribution of amino acids in Friedreich's ataxia brains.", "content": "The distribution of amino acids in two Friedreich's ataxia brains is compared to 4 control brains. Glutamate and GABA were decreased in the cerebellar hemispheres and/or in the vermis. Taurine concentrations were uniformly elevated throughout the areas studied. Taurine/glutamate and taurine/GABA ratios were consistently elevated in Friedreich's ataxia brains, whereas glutamate/glutamine ratios were consistently decreased (with normal glutamine concentrations).", "contents": "Regional distribution of amino acids in Friedreich's ataxia brains. The distribution of amino acids in two Friedreich's ataxia brains is compared to 4 control brains. Glutamate and GABA were decreased in the cerebellar hemispheres and/or in the vermis. Taurine concentrations were uniformly elevated throughout the areas studied. Taurine/glutamate and taurine/GABA ratios were consistently elevated in Friedreich's ataxia brains, whereas glutamate/glutamine ratios were consistently decreased (with normal glutamine concentrations)."}
{"id": "PMID:487320", "title": "Dicarboxylic amino acid uptake in normal, Friedreich's ataxia, and dicarboxylic aminoaciduria fibroblasts.", "content": "Glutamic and aspartic acid uptake was measured in skin fibroblasts from patients with Friedreich's Ataxia, dicarboxylic aminoaciduria, and normal individuals. The results showed no difference in uptake kinetics of either dicarboxylic amino acids between Friedreich's Ataxia and normal cells, but reduced uptake velocities in dicarboxylic aminoaciduria fibroblasts. Friedreich's Ataxia fibroblasts were, however, less calcium-dependent and more magnesium and phosphate-dependent than controls in glucose-free incubation mixture. This difference might be related to some degree of glucose intolerance by Friedreich's Ataxia fibroblasts in culture.", "contents": "Dicarboxylic amino acid uptake in normal, Friedreich's ataxia, and dicarboxylic aminoaciduria fibroblasts. Glutamic and aspartic acid uptake was measured in skin fibroblasts from patients with Friedreich's Ataxia, dicarboxylic aminoaciduria, and normal individuals. The results showed no difference in uptake kinetics of either dicarboxylic amino acids between Friedreich's Ataxia and normal cells, but reduced uptake velocities in dicarboxylic aminoaciduria fibroblasts. Friedreich's Ataxia fibroblasts were, however, less calcium-dependent and more magnesium and phosphate-dependent than controls in glucose-free incubation mixture. This difference might be related to some degree of glucose intolerance by Friedreich's Ataxia fibroblasts in culture."}
{"id": "PMID:487321", "title": "Pilot studies on membranes and some transport mechanisms in Friedreich's ataxia.", "content": "The observed anomalies in high density lipoproteins in Friedreich's ataxia led us to investigate the state of cellular membranes in this illness. As a preliminary screening program, we studied the shape of erythrocytes; the phospholipid content of platelets and the transport properties of these membranes as indirectly reflected in the absorption of Vit E and the renal handling of orally injected taurine. All these investigations were normal, except for a tendency towards more echinocytes in Friedreich's ataxia and the insignificant increase in taurine urinary excretion after an oral load. We concluded that the possible membrane abnormalities are not major and will have to be searched for with more subtle and specific tests.", "contents": "Pilot studies on membranes and some transport mechanisms in Friedreich's ataxia. The observed anomalies in high density lipoproteins in Friedreich's ataxia led us to investigate the state of cellular membranes in this illness. As a preliminary screening program, we studied the shape of erythrocytes; the phospholipid content of platelets and the transport properties of these membranes as indirectly reflected in the absorption of Vit E and the renal handling of orally injected taurine. All these investigations were normal, except for a tendency towards more echinocytes in Friedreich's ataxia and the insignificant increase in taurine urinary excretion after an oral load. We concluded that the possible membrane abnormalities are not major and will have to be searched for with more subtle and specific tests."}
{"id": "PMID:487323", "title": "Friedreich's ataxia 1979: an overview.", "content": "This overview summarizes the investigations carried out during the second part of Phase Two of the Quebec Cooperative Study of Friedreich's Ataxia. These investigations outline in more details the fundamental role played by an abnormality in the fatty acid composition (deficient linoleic acid, 18:2) of the cholesterol esters of high density lipoproteins (HDL) in the phenotypic expression of the disease. They postulate a defective incorporation of linoleic acid to surface phosphatidylcholine of chylomicrons and consequent relative and absolute decreases in lipoprotein protein components because of overpacking with defective cholesteryl esters. Secondarily to these changes, the postulated lack of activation of the lipoamide dehydrogenase (LAD) of the pyruvate dehydrogenase (PDH) complex could result in slow pyruvate oxidation, glucose intolerance, deficient synthesis of acetylcholine, and depletion of glutamic and aspartic acid pools. In parallel, abnormal phosphatidyl-choline molecules could be incorporated to membranes, resulting in specific defects in some functions of these membranes, including transport of calcium and/or taurine and myelinization. The framework of an understanding of Friedreich's ataxia is now available, but much fundamental and clinical work remains to be done to fill in and prove each one of these postulated steps.", "contents": "Friedreich's ataxia 1979: an overview. This overview summarizes the investigations carried out during the second part of Phase Two of the Quebec Cooperative Study of Friedreich's Ataxia. These investigations outline in more details the fundamental role played by an abnormality in the fatty acid composition (deficient linoleic acid, 18:2) of the cholesterol esters of high density lipoproteins (HDL) in the phenotypic expression of the disease. They postulate a defective incorporation of linoleic acid to surface phosphatidylcholine of chylomicrons and consequent relative and absolute decreases in lipoprotein protein components because of overpacking with defective cholesteryl esters. Secondarily to these changes, the postulated lack of activation of the lipoamide dehydrogenase (LAD) of the pyruvate dehydrogenase (PDH) complex could result in slow pyruvate oxidation, glucose intolerance, deficient synthesis of acetylcholine, and depletion of glutamic and aspartic acid pools. In parallel, abnormal phosphatidyl-choline molecules could be incorporated to membranes, resulting in specific defects in some functions of these membranes, including transport of calcium and/or taurine and myelinization. The framework of an understanding of Friedreich's ataxia is now available, but much fundamental and clinical work remains to be done to fill in and prove each one of these postulated steps."}
{"id": "PMID:487322", "title": "Erythrocyte membrane lipids in Friedreich's ataxia.", "content": "In a study of the lipid composition of erythrocyte membranes in Friedreich's ataxia, the concentration of the major membrane components (phospholipids, cholesterol and protein) in ataxic patients, family members, and control subjects were found to be the same. The total fatty acid distribution was also normal. However, an altered distribution of phospholipid classes in erythrocytes was noted (an increase of PI + PS and a decrease of PE in Friedreich's ataxia patients).", "contents": "Erythrocyte membrane lipids in Friedreich's ataxia. In a study of the lipid composition of erythrocyte membranes in Friedreich's ataxia, the concentration of the major membrane components (phospholipids, cholesterol and protein) in ataxic patients, family members, and control subjects were found to be the same. The total fatty acid distribution was also normal. However, an altered distribution of phospholipid classes in erythrocytes was noted (an increase of PI + PS and a decrease of PE in Friedreich's ataxia patients)."}
{"id": "PMID:487324", "title": "Spinal epidural infection.", "content": "Thirty cases of spinal epidural abscess are reviewed with particular emphasis on mode of presentation and results of treatment. In spite of repeated exhortations in the literature for earlier diagnosis, the results remain unsatisfactory because many patients have progressed to severe or complete paralysis before the correct diagnosis is made and surgical treatment carried out.", "contents": "Spinal epidural infection. Thirty cases of spinal epidural abscess are reviewed with particular emphasis on mode of presentation and results of treatment. In spite of repeated exhortations in the literature for earlier diagnosis, the results remain unsatisfactory because many patients have progressed to severe or complete paralysis before the correct diagnosis is made and surgical treatment carried out."}
{"id": "PMID:487325", "title": "[Brain tumors in children in a French Canadian environment].", "content": "Between 1964 and 1976, we have seen 197 cases of cerebral tumors in children. We have studied the frequency of each histological group of tumors, their localization, the results of the clinical investigation, their clinical manifestations and the prognosis for each group. This study has confirmed that in children brain tumors are more frequently localized in the posterior fossa (56.4%). As in other series, tumors derived from the glia represent nearly 80% of the total. Clinically, increased intro-cranial pressure is the most frequent manifestation of posterior fossa lesion (75% of cases) while cerebellar involvement was seen in 60% of these children. Brain lesions and supratentorial tumors are more insidious in their presentation and their clinical manifestations more diversified. The EEG appeared useful in the investigation of brain tumors and showed irregularities in 78% of our patients. However, a more specific diagnosis requirelography. As in previous series, the mortality rate remained high in the children we have followed, particularly for some groups of tumors (glioblastoma, medulloblastoma, brain stem lesions). It is to be hoped that with the introduction of the CT Scan, the improvement of neurosurgical techniques and the use of immuno and chemotherapy, the prognosis may be improved.", "contents": "[Brain tumors in children in a French Canadian environment]. Between 1964 and 1976, we have seen 197 cases of cerebral tumors in children. We have studied the frequency of each histological group of tumors, their localization, the results of the clinical investigation, their clinical manifestations and the prognosis for each group. This study has confirmed that in children brain tumors are more frequently localized in the posterior fossa (56.4%). As in other series, tumors derived from the glia represent nearly 80% of the total. Clinically, increased intro-cranial pressure is the most frequent manifestation of posterior fossa lesion (75% of cases) while cerebellar involvement was seen in 60% of these children. Brain lesions and supratentorial tumors are more insidious in their presentation and their clinical manifestations more diversified. The EEG appeared useful in the investigation of brain tumors and showed irregularities in 78% of our patients. However, a more specific diagnosis requirelography. As in previous series, the mortality rate remained high in the children we have followed, particularly for some groups of tumors (glioblastoma, medulloblastoma, brain stem lesions). It is to be hoped that with the introduction of the CT Scan, the improvement of neurosurgical techniques and the use of immuno and chemotherapy, the prognosis may be improved."}
{"id": "PMID:487326", "title": "Epilepsy and pregnancy.", "content": "The management of the pregnant epileptic requires close cooperation between the neurologist and obstetrician. To prevent complications, knowledge is required about the natural history of epilepsy during pregnancy, the possible teratogenic effects of antiepileptic drugs, and changes in their absorption, biotransformation, and excretion. Close plasma antiepileptic drug monitoring is required because of the change in the handling of antiepileptic drugs during pregnancy. The treatment of status epilepticus with intravenous phenytoin is effective. Drug interactions which may lead to toxic plasma levels of some drugs and subtherapeutic plasma levels of others should be anticipated. The risk of problems resulting from antiepileptic drug therapy during pregnancy appears to be minor, provided that proper medical supervision is available. Newer antiepileptic drugs should not be administered to the pregnant epileptic until their safety in pregnancy is fully established.", "contents": "Epilepsy and pregnancy. The management of the pregnant epileptic requires close cooperation between the neurologist and obstetrician. To prevent complications, knowledge is required about the natural history of epilepsy during pregnancy, the possible teratogenic effects of antiepileptic drugs, and changes in their absorption, biotransformation, and excretion. Close plasma antiepileptic drug monitoring is required because of the change in the handling of antiepileptic drugs during pregnancy. The treatment of status epilepticus with intravenous phenytoin is effective. Drug interactions which may lead to toxic plasma levels of some drugs and subtherapeutic plasma levels of others should be anticipated. The risk of problems resulting from antiepileptic drug therapy during pregnancy appears to be minor, provided that proper medical supervision is available. Newer antiepileptic drugs should not be administered to the pregnant epileptic until their safety in pregnancy is fully established."}
{"id": "PMID:487327", "title": "Decrease in the rate of protein synthesis by polysomes from cultured fibroblasts of patients and carriers with Duchenne muscular dystrophy.", "content": "Polysomes extracted from cultured fibroblast cells isolated from patients with Duchenne muscular dystrophy (DMD), carriers of the disease, and normal controls were used for in vitro measurement of protein synthesis in a wheat germ extract system. It was observed that polysomes from patients and carriers (seven of each aged 17 years or older) exhibited a 3-fold and a 1.5-fold decrease in the rate of protein synthesis, respectively, as compared with controls. These results are discussed with a view to developing a sensitive and easily available assay for the detection of DMD carriers.", "contents": "Decrease in the rate of protein synthesis by polysomes from cultured fibroblasts of patients and carriers with Duchenne muscular dystrophy. Polysomes extracted from cultured fibroblast cells isolated from patients with Duchenne muscular dystrophy (DMD), carriers of the disease, and normal controls were used for in vitro measurement of protein synthesis in a wheat germ extract system. It was observed that polysomes from patients and carriers (seven of each aged 17 years or older) exhibited a 3-fold and a 1.5-fold decrease in the rate of protein synthesis, respectively, as compared with controls. These results are discussed with a view to developing a sensitive and easily available assay for the detection of DMD carriers."}
{"id": "PMID:487328", "title": "Painful tonic seizures in multiple sclerosis: localization of a lesion.", "content": "The history of a patient with painful tonic seizures in association with multiple sclerosis is related. These seizures have been thought by most authors to be spinal in origin. This case is reported because of the localization of a supratentorial lesion, clinically and on CT scan.", "contents": "Painful tonic seizures in multiple sclerosis: localization of a lesion. The history of a patient with painful tonic seizures in association with multiple sclerosis is related. These seizures have been thought by most authors to be spinal in origin. This case is reported because of the localization of a supratentorial lesion, clinically and on CT scan."}
{"id": "PMID:487329", "title": "Cold induced rhabdomyolysis in carnitine palmyityl transferase deficiency.", "content": "A case of carnitine palmityl transferase deficiency in skeletal muscle is described. The usual symptoms associated with this disease (recurrent muscle cramps or pain and pigmenturia) were observed but sudden exposure to cold precipitate rhabdomyolysis in this patient.", "contents": "Cold induced rhabdomyolysis in carnitine palmyityl transferase deficiency. A case of carnitine palmityl transferase deficiency in skeletal muscle is described. The usual symptoms associated with this disease (recurrent muscle cramps or pain and pigmenturia) were observed but sudden exposure to cold precipitate rhabdomyolysis in this patient."}
{"id": "PMID:487330", "title": "Human cysticercosis: a probable case of cerebral cysticercosis with generalized subcutaneous nodular lesions.", "content": "A case is reported in Montreal of human cysticercosis in a 44-year-old man who emigrated from Italy in 1956. Numerous subcutaneous nodules were found throughout his body. X-ray of his thighs and chest showed oblong calcific densities measuring 1 x 0.5 cm in size. Examination of an excised nodule from the right biceps revealed a cysticercus larva morphologically similar to Cysticercus cellulosae although the scolex lacked hooks. On the basis of the intensity of infection, focal and generalized epileptic seizures, changes in the CSF, and well demarcated lucencies observed in brain scan, cerebral cysticercosis was considered the most likely diagnosis.", "contents": "Human cysticercosis: a probable case of cerebral cysticercosis with generalized subcutaneous nodular lesions. A case is reported in Montreal of human cysticercosis in a 44-year-old man who emigrated from Italy in 1956. Numerous subcutaneous nodules were found throughout his body. X-ray of his thighs and chest showed oblong calcific densities measuring 1 x 0.5 cm in size. Examination of an excised nodule from the right biceps revealed a cysticercus larva morphologically similar to Cysticercus cellulosae although the scolex lacked hooks. On the basis of the intensity of infection, focal and generalized epileptic seizures, changes in the CSF, and well demarcated lucencies observed in brain scan, cerebral cysticercosis was considered the most likely diagnosis."}
{"id": "PMID:487333", "title": "The analysis of a case of dysmorfophobia.", "content": "A case history is presented of a 16 year old adopted girl, diagnosed as having dysmorfophobia and successfully treated by psychoanalytically-oriented psychotherapy, during which time much material for discussion and interpretation was provided by the patient's dreams. The psychodynamics of the case are explored. In this case, dysmorfophobia is seen as a facade covering up the patient's unconscious urge to yield to sexual temptation. Clinically, the case may be seen and classified as follows: It may be a syndrome or a cluster of symptoms that do not currently exist in the standard psychiatric nomenclature. The diagnosis of a personality trait disorder in a not yet fully structured personality might be a second alternative diagnosis. The author, however, is inclined to consider the case as \"transitional\" or \"potential\" emotional disturbance, in accordance with Edward Glover's views (4).", "contents": "The analysis of a case of dysmorfophobia. A case history is presented of a 16 year old adopted girl, diagnosed as having dysmorfophobia and successfully treated by psychoanalytically-oriented psychotherapy, during which time much material for discussion and interpretation was provided by the patient's dreams. The psychodynamics of the case are explored. In this case, dysmorfophobia is seen as a facade covering up the patient's unconscious urge to yield to sexual temptation. Clinically, the case may be seen and classified as follows: It may be a syndrome or a cluster of symptoms that do not currently exist in the standard psychiatric nomenclature. The diagnosis of a personality trait disorder in a not yet fully structured personality might be a second alternative diagnosis. The author, however, is inclined to consider the case as \"transitional\" or \"potential\" emotional disturbance, in accordance with Edward Glover's views (4)."}
{"id": "PMID:487334", "title": "On knowing what you are not supposed to know and feeling what you are not supposed to feel.", "content": "Much psychopathology stems from \"impressions, scenes, and experiences\" of childhood having, apparently, been forgotten yet continuing to influence thought, feeling and action. The problem of how best to conceptualize the processes responsible for shutting memories away is eased by drawing on the findings and concepts of cognitive psychology. In explaining both the nature of the material shut away and the causes of its being so, attention is called to the role that a child's parents play, wittingly or unwittingly. Children not infrequently observe scenes their parents would prefer they did not observe; they form impressions their parents would prefer they did not form; and they have experiences their parents would like to believe they have not had. Examples are given of parents who seek to disconfirm their child's observations of events, to disapprove or condemn their natural emotional responses to distressing situations, and who discourage their children from registering aspects of their (the parents') personalities and behaviour. Pressure exerted on the children to conform to their parents' wishes can be crude or subtle, but its effectiveness depends always on the child's insistent desire to be loved and protected.", "contents": "On knowing what you are not supposed to know and feeling what you are not supposed to feel. Much psychopathology stems from \"impressions, scenes, and experiences\" of childhood having, apparently, been forgotten yet continuing to influence thought, feeling and action. The problem of how best to conceptualize the processes responsible for shutting memories away is eased by drawing on the findings and concepts of cognitive psychology. In explaining both the nature of the material shut away and the causes of its being so, attention is called to the role that a child's parents play, wittingly or unwittingly. Children not infrequently observe scenes their parents would prefer they did not observe; they form impressions their parents would prefer they did not form; and they have experiences their parents would like to believe they have not had. Examples are given of parents who seek to disconfirm their child's observations of events, to disapprove or condemn their natural emotional responses to distressing situations, and who discourage their children from registering aspects of their (the parents') personalities and behaviour. Pressure exerted on the children to conform to their parents' wishes can be crude or subtle, but its effectiveness depends always on the child's insistent desire to be loved and protected."}
{"id": "PMID:487337", "title": "Geropsychiatry in Indian culture.", "content": "Geriatric psychiatry will be increasingly important in years to come as the care of the elderly becomes a health problem in India. Those aged 60 and over contribute to 6% of the country's population at present. The life expectancy of an average Indian has increased from 24 years in 1900 to 53 in 1971. Improved health care promises longevity, but social and economic conditions like poverty, breakup of the joint family system and poor services, specifically for the aged, pose a psychiatric threat to them. Organic diseases and effective disorders form the bulk of psychiatric illness in the aged. Suggestions for research in areas such as social, cultural and changing attitudes, and national economics, have been suggested as a promising field for preventive geriatric services. Adding life to years is more important than adding years to life. The paper offers some references to the studies on geriatric psychiatry in India and discusses cultural factors affecting the aged.", "contents": "Geropsychiatry in Indian culture. Geriatric psychiatry will be increasingly important in years to come as the care of the elderly becomes a health problem in India. Those aged 60 and over contribute to 6% of the country's population at present. The life expectancy of an average Indian has increased from 24 years in 1900 to 53 in 1971. Improved health care promises longevity, but social and economic conditions like poverty, breakup of the joint family system and poor services, specifically for the aged, pose a psychiatric threat to them. Organic diseases and effective disorders form the bulk of psychiatric illness in the aged. Suggestions for research in areas such as social, cultural and changing attitudes, and national economics, have been suggested as a promising field for preventive geriatric services. Adding life to years is more important than adding years to life. The paper offers some references to the studies on geriatric psychiatry in India and discusses cultural factors affecting the aged."}
{"id": "PMID:487338", "title": "Depression, witchcraft beliefs and super-ego development in pre-literate societies.", "content": "When guilt feelings and self-accusations made their first appearance as symptoms of depression in Europe and Africa, as noted in a previous paper, this followed a period of heightened witchcraft beliefs in both locations and was sometimes first noticed in the form of voluntary witchcraft confessions, raising the question of a possible connection between the two types of change. Using the concepts of \"group ego\" and \"group super-ego\" to which Eric Wittkower has contributed, it is suggested that the heightened witchcraft beliefs were a defence against an individualizing change which would eventually lead to the inward turning to aggression and reproach and hence to the symptoms mentioned.", "contents": "Depression, witchcraft beliefs and super-ego development in pre-literate societies. When guilt feelings and self-accusations made their first appearance as symptoms of depression in Europe and Africa, as noted in a previous paper, this followed a period of heightened witchcraft beliefs in both locations and was sometimes first noticed in the form of voluntary witchcraft confessions, raising the question of a possible connection between the two types of change. Using the concepts of \"group ego\" and \"group super-ego\" to which Eric Wittkower has contributed, it is suggested that the heightened witchcraft beliefs were a defence against an individualizing change which would eventually lead to the inward turning to aggression and reproach and hence to the symptoms mentioned."}
{"id": "PMID:487339", "title": "Psychosomatic illness concept and psychotherapy among the Akan of Ghana.", "content": "The example of the Akan in Ghana shows that western medicine has no psychotherapeutic alternative to offer the so-called \"developing countries\". The traditional healing methods existing in such countries must be preserved and researched in order to maintain them at least at their present standard (9). This task, as well as that of developing these methods over the coming decades to keep pace with the cultural development process, is one that devolves upon the young scientists and doctors of such countries. This in turn requires further study of socialization values and norms, the potential resultant conflicts, the psychotherapeutic conflict-solving processes designed to obviate them and the forces at work in such psychotherapy.", "contents": "Psychosomatic illness concept and psychotherapy among the Akan of Ghana. The example of the Akan in Ghana shows that western medicine has no psychotherapeutic alternative to offer the so-called \"developing countries\". The traditional healing methods existing in such countries must be preserved and researched in order to maintain them at least at their present standard (9). This task, as well as that of developing these methods over the coming decades to keep pace with the cultural development process, is one that devolves upon the young scientists and doctors of such countries. This in turn requires further study of socialization values and norms, the potential resultant conflicts, the psychotherapeutic conflict-solving processes designed to obviate them and the forces at work in such psychotherapy."}
{"id": "PMID:487342", "title": "The influence of the supervision of psychotherapy upon being therapeutic. I: Introduction and background to the supervisory relationship.", "content": "In individual psychotherapy which derives from psychoanalysis, the most important teaching tool is individual supervision. This is in keeping with the fact that close person-toperson contact between student and teacher is the most effective mode of teaching in a university. The supervisor's first task is to transmit a body of knowledge, about psychotherapy, to the student. His second task is to give the student the benefit of his experience around clinical problems or questions. His third task is to assist the student in the development of certain attitudes which are valuable and even essential for a psychotherapist. This latter task is accomplished largely via \"reality identification\".", "contents": "The influence of the supervision of psychotherapy upon being therapeutic. I: Introduction and background to the supervisory relationship. In individual psychotherapy which derives from psychoanalysis, the most important teaching tool is individual supervision. This is in keeping with the fact that close person-toperson contact between student and teacher is the most effective mode of teaching in a university. The supervisor's first task is to transmit a body of knowledge, about psychotherapy, to the student. His second task is to give the student the benefit of his experience around clinical problems or questions. His third task is to assist the student in the development of certain attitudes which are valuable and even essential for a psychotherapist. This latter task is accomplished largely via \"reality identification\"."}
{"id": "PMID:487343", "title": "The influence of the supervision of psychotherapy upon being therapeutic. II: Modes of influence of the supervisory relationship.", "content": "Through supervision, the student develops those innate personal characteristics which are highly important in being therapeutic. In making conscious use of \"reality identification\" by the student with his teacher, we must take care to avoid certain pitfalls. What is not wanted is transference identification. To help avoid this, we must select appropriate teachers as supervisors. The student should also have more than one supervisor, as this tends to protect against the development of overidealization on the part of the student, of omnipotence on the part of the teacher. Personal characteristics of both students and supervisors are extremely important. A good supervisory experience is the most likely vehicle for developing those qualities in the student which will, in time, lead him to function most therapeutically.", "contents": "The influence of the supervision of psychotherapy upon being therapeutic. II: Modes of influence of the supervisory relationship. Through supervision, the student develops those innate personal characteristics which are highly important in being therapeutic. In making conscious use of \"reality identification\" by the student with his teacher, we must take care to avoid certain pitfalls. What is not wanted is transference identification. To help avoid this, we must select appropriate teachers as supervisors. The student should also have more than one supervisor, as this tends to protect against the development of overidealization on the part of the student, of omnipotence on the part of the teacher. Personal characteristics of both students and supervisors are extremely important. A good supervisory experience is the most likely vehicle for developing those qualities in the student which will, in time, lead him to function most therapeutically."}
{"id": "PMID:487347", "title": "Patient management in disulfiram implant therapy.", "content": "Data from 100 disulfiram implant, placebo, and no-implant control patients demonstrate the effectiveness of the disulfiram implant in keeping the alcoholic dry. However, the superiority of the disulfiram implant group over the placebo group is interpreted as evidence of a pharmacological component to the procedure operating independent of the disulfiram-ethanol reaction (DER). Hypotheses involving inhibition of aldehyde dehydrogenase or inhibition of dopamine-beta-hydroxylase are offered as mechanisms by which the pharmacological effect may be mediated. Finally, in view of the low (approximately 0.5) probability with which a DER follows ingestion of alcohol by a disulfiram implant patient, it is suggested that the approach to patient management should be changed to maximize the effectiveness of the disulfiram implant procedure. Guidelines are given.", "contents": "Patient management in disulfiram implant therapy. Data from 100 disulfiram implant, placebo, and no-implant control patients demonstrate the effectiveness of the disulfiram implant in keeping the alcoholic dry. However, the superiority of the disulfiram implant group over the placebo group is interpreted as evidence of a pharmacological component to the procedure operating independent of the disulfiram-ethanol reaction (DER). Hypotheses involving inhibition of aldehyde dehydrogenase or inhibition of dopamine-beta-hydroxylase are offered as mechanisms by which the pharmacological effect may be mediated. Finally, in view of the low (approximately 0.5) probability with which a DER follows ingestion of alcohol by a disulfiram implant patient, it is suggested that the approach to patient management should be changed to maximize the effectiveness of the disulfiram implant procedure. Guidelines are given."}
{"id": "PMID:487348", "title": "The mother tongue in psychotherapy.", "content": "The psychotherapy of three women with neurotic and adaptational problems was conducted using their mother tongue, German, and emphasis is placed on the facilitation of the therapeutic process by this method. It is felt that transference may be readily formed, there is less resistance to examining conflict areas, and the use of the acquired language as a defense against anxiety can be avoided. Several other authors have noted similar effects of therapy in the mother tongue. Various aspects of the literature are discussed, particularly focusing on the affective and cognitive differences between the primary and acquired languages as they may influence the psychotherapy.", "contents": "The mother tongue in psychotherapy. The psychotherapy of three women with neurotic and adaptational problems was conducted using their mother tongue, German, and emphasis is placed on the facilitation of the therapeutic process by this method. It is felt that transference may be readily formed, there is less resistance to examining conflict areas, and the use of the acquired language as a defense against anxiety can be avoided. Several other authors have noted similar effects of therapy in the mother tongue. Various aspects of the literature are discussed, particularly focusing on the affective and cognitive differences between the primary and acquired languages as they may influence the psychotherapy."}
{"id": "PMID:487349", "title": "A note on counter-transference and anti-Semitism.", "content": "After a brief introduction where the meaning of racism is alluded to, a clinical vignette is presented. A patient who has suppressed her antisemitic feelings is being treated by a Jewish psychiatrist. The therapist's possible counter-transference motives in treating the patient are explored, and a workable solution is offered.", "contents": "A note on counter-transference and anti-Semitism. After a brief introduction where the meaning of racism is alluded to, a clinical vignette is presented. A patient who has suppressed her antisemitic feelings is being treated by a Jewish psychiatrist. The therapist's possible counter-transference motives in treating the patient are explored, and a workable solution is offered."}
{"id": "PMID:487350", "title": "The incubus syndrome. A variant of erotomania.", "content": "The term \"incubus syndrome\" is proposed to describe patients suffering from the delusion that they have been sexually approached at night by an unseen lover. Two cases characterized by co-existence of erotomania and schizophrenic sexual delusions are described. The patient's behaviour secondary to the defusion is discussed from the point of view of social significance as well as potential dangerousness.", "contents": "The incubus syndrome. A variant of erotomania. The term \"incubus syndrome\" is proposed to describe patients suffering from the delusion that they have been sexually approached at night by an unseen lover. Two cases characterized by co-existence of erotomania and schizophrenic sexual delusions are described. The patient's behaviour secondary to the defusion is discussed from the point of view of social significance as well as potential dangerousness."}
{"id": "PMID:487351", "title": "Some determinants of affective symptoms in epileptics.", "content": "Forty-two epileptics were given a psychiatric interview and completed five questionnaires. Current affective symptoms were significantly associated with a past history of neurotic disorder but not with temporal lobe epilepsy.", "contents": "Some determinants of affective symptoms in epileptics. Forty-two epileptics were given a psychiatric interview and completed five questionnaires. Current affective symptoms were significantly associated with a past history of neurotic disorder but not with temporal lobe epilepsy."}
{"id": "PMID:487352", "title": "The psychoanalytic theory of depression. Part II--The major themes.", "content": "In this second part of a review of the psychoanalytic theory of depression the major themes of the theory, as they appear in the works of the major contributors, are discussed. It is difficult to approach the complexities and ambiguities of psychoanalytic theory in general, and the theory of depression in particular, without an historical perspective. Accordingly, the author decided to group the major themes of the theory under three headings: Instinct Theory, Structural Theory, Object Relations Theory. The themes included under Instinct Theory are constitutional factors, aggression and orality. Under the heading of Structural Theory those themes associated with the ego, with the concepts of narcissism and the self, and finally those associated with the superego are discussed. It is of note that under narcissism the work of both Kohut and Kernberg is considered, including its relevance to any investigation of depression. The concepts of object loss and object failure are discussed under the heading of Object Relations Theory. It is suggested that a psychoanalytic approach has much to offer both the clinician and the researcher in their attempts to develop a comprehensive theory to explain the protean manifestations of human depression.", "contents": "The psychoanalytic theory of depression. Part II--The major themes. In this second part of a review of the psychoanalytic theory of depression the major themes of the theory, as they appear in the works of the major contributors, are discussed. It is difficult to approach the complexities and ambiguities of psychoanalytic theory in general, and the theory of depression in particular, without an historical perspective. Accordingly, the author decided to group the major themes of the theory under three headings: Instinct Theory, Structural Theory, Object Relations Theory. The themes included under Instinct Theory are constitutional factors, aggression and orality. Under the heading of Structural Theory those themes associated with the ego, with the concepts of narcissism and the self, and finally those associated with the superego are discussed. It is of note that under narcissism the work of both Kohut and Kernberg is considered, including its relevance to any investigation of depression. The concepts of object loss and object failure are discussed under the heading of Object Relations Theory. It is suggested that a psychoanalytic approach has much to offer both the clinician and the researcher in their attempts to develop a comprehensive theory to explain the protean manifestations of human depression."}
{"id": "PMID:487358", "title": "A serological survey of Leptospira interrogans serotype pomona in Saskatchewan horses.", "content": "Leptospira interrogans serotype pomona antibody titres of 1:100 or greater were detected in 12.8% of 408 adult horses from seven of eight sampled herds in Saskatchewan. The geographical distribution of the seropositive horses was widespread throughout the agricultural area of the province. The geographical distribution and the cumulative increase in prevalence with age suggested that serotype pomona is enzootic in the equine population of Saskatchewan.", "contents": "A serological survey of Leptospira interrogans serotype pomona in Saskatchewan horses. Leptospira interrogans serotype pomona antibody titres of 1:100 or greater were detected in 12.8% of 408 adult horses from seven of eight sampled herds in Saskatchewan. The geographical distribution of the seropositive horses was widespread throughout the agricultural area of the province. The geographical distribution and the cumulative increase in prevalence with age suggested that serotype pomona is enzootic in the equine population of Saskatchewan."}
{"id": "PMID:487359", "title": "The effect of retained placenta and metritis complex on reproductive performance in dairy cattle -- a case control study.", "content": "A study of the computer stored records of 293 dairy cows and 652 calvings reveals the effects of retained placenta and metritis complex on reproductive performance. The overall incidence rate of retained placenta was 11.2%. Retained placenta was 4.6 times more likely to occur following twin births than following single births. Most cases of retained placenta occurred during the fall. Forty-five percent of that seasonal increase was explained by an increased number of calvings. Metritis complex was diagnosed following 54.8% of retained placenta cases. Retained placenta alone did not significantly impair reproductive performance. Metritis complex, in the presence or absence of retained placenta, caused a significant (P</=0.05) increase in days open, services per conception, calving to first heat intervals and days from calving to first service. There is an indication (interaction P</=0.1) that cows with both retained placenta and metritis complex are more severely affected than cows with either retained placenta or metritis complex alone. The influence of retained placenta on fertility appears to depend on the proportion of cows with retained placenta that have metritis complex.", "contents": "The effect of retained placenta and metritis complex on reproductive performance in dairy cattle -- a case control study. A study of the computer stored records of 293 dairy cows and 652 calvings reveals the effects of retained placenta and metritis complex on reproductive performance. The overall incidence rate of retained placenta was 11.2%. Retained placenta was 4.6 times more likely to occur following twin births than following single births. Most cases of retained placenta occurred during the fall. Forty-five percent of that seasonal increase was explained by an increased number of calvings. Metritis complex was diagnosed following 54.8% of retained placenta cases. Retained placenta alone did not significantly impair reproductive performance. Metritis complex, in the presence or absence of retained placenta, caused a significant (P</=0.05) increase in days open, services per conception, calving to first heat intervals and days from calving to first service. There is an indication (interaction P</=0.1) that cows with both retained placenta and metritis complex are more severely affected than cows with either retained placenta or metritis complex alone. The influence of retained placenta on fertility appears to depend on the proportion of cows with retained placenta that have metritis complex."}
{"id": "PMID:487360", "title": "Prevalence and treatment of tapeworms in horses.", "content": "A study was initiated to determine the prevalence of tapeworms in horses in Southern Ontario and to investigate the efficacy of pyrantel pamoate, niclosamide and mebendazole. Fecal samples were taken from 580 horses of various breeds, ages and sexes in 24 locations and Anoplocephala perfoliata was found in 13.6%. This was regarded as a minimum, the true rate being probably significantly higher and the reasons for this are discussed. A brief review of the life cycle and effects of tapeworms in horses and a comparison of two flotation techniques for the diagnosis of A. perfoliata eggs in feces is given. Pyrantel pamoate, niclosamide and mebendazole were compared for efficacy in field and critical trials. In field trials, pyrantel base and niclosamide at 6.6 and 50 mg/kg respectively were found to be effective, but in critical trials their efficacy was poor, 15 and 5.6% respectively. These anthelmintics at these dose rates caused only an elimination of the terminal egg bearing segments and were without significant effect on the entire tapeworm. When pyrantel base was administered at 13.2 and 19.8 mg/kg (twice and three times the therapeutic dose rate for nematodes respectively) the efficacy was 97.8 and 100%. It would appear that pyrantel pamoate administered at 13.2 mg pyrantel base/kg is an effective therapeutic dose for tapeworms in horses. Further dose titration studies are needed for niclosamide. Mebendazole was without effect at up to four (35.2 mg/kg) times the therapeutic dose for nematodes.", "contents": "Prevalence and treatment of tapeworms in horses. A study was initiated to determine the prevalence of tapeworms in horses in Southern Ontario and to investigate the efficacy of pyrantel pamoate, niclosamide and mebendazole. Fecal samples were taken from 580 horses of various breeds, ages and sexes in 24 locations and Anoplocephala perfoliata was found in 13.6%. This was regarded as a minimum, the true rate being probably significantly higher and the reasons for this are discussed. A brief review of the life cycle and effects of tapeworms in horses and a comparison of two flotation techniques for the diagnosis of A. perfoliata eggs in feces is given. Pyrantel pamoate, niclosamide and mebendazole were compared for efficacy in field and critical trials. In field trials, pyrantel base and niclosamide at 6.6 and 50 mg/kg respectively were found to be effective, but in critical trials their efficacy was poor, 15 and 5.6% respectively. These anthelmintics at these dose rates caused only an elimination of the terminal egg bearing segments and were without significant effect on the entire tapeworm. When pyrantel base was administered at 13.2 and 19.8 mg/kg (twice and three times the therapeutic dose rate for nematodes respectively) the efficacy was 97.8 and 100%. It would appear that pyrantel pamoate administered at 13.2 mg pyrantel base/kg is an effective therapeutic dose for tapeworms in horses. Further dose titration studies are needed for niclosamide. Mebendazole was without effect at up to four (35.2 mg/kg) times the therapeutic dose for nematodes."}
{"id": "PMID:487361", "title": "Atypical interstitial pneumonia in cattle.", "content": "Atypical interstitial pneumonia is described as two clinical syndromes in young cattle. One syndrome occurs in animals which have clinical evidence of pneumonic pasteurellosis, responds initially to treatment for one to two days and then develops acute signs of atypical interstitial pneumonia. The second syndrome involves acute respiratory distress in young calves due to atypical interstitial pneumonia with antecedents of enzootic pneumonia. The postmortem lesions are described along with discussion of the possible pathogenesis of the condition and treatment.", "contents": "Atypical interstitial pneumonia in cattle. Atypical interstitial pneumonia is described as two clinical syndromes in young cattle. One syndrome occurs in animals which have clinical evidence of pneumonic pasteurellosis, responds initially to treatment for one to two days and then develops acute signs of atypical interstitial pneumonia. The second syndrome involves acute respiratory distress in young calves due to atypical interstitial pneumonia with antecedents of enzootic pneumonia. The postmortem lesions are described along with discussion of the possible pathogenesis of the condition and treatment."}
{"id": "PMID:487362", "title": "Structure of the water-insoluble alpha-D-glucan of Streptococcus salivarius HHT.", "content": "Water-insoluble, non-adherent alpha-D-glucans have been obtained from Streptococcus salivarius HHT under two sets of conditions: from a growing culture, or synthesized enzymically by using a glucosyltransferase. In the former case, the glucan ([alpha]D + 197 degrees) was shown by methylation analysis to have a slightly branched structure containing a relatively high proportion (80%) of (1 leads to 3)-alpha-D-glucosidic linkages, together with small proportions of (1 leads to 6)- and (1 leads to 4)-alpha-D-glucosidic linkages. The enzymically synthesized glucan had a much less-branched structure, containing 88% of (1 leads to 3)-alpha-D-glucosidic linkages. Both glucans, on Smith degradation (sequential periodate oxidation, borohydride reduction, and mild acid hydrolysis), gave linear, (1 leads to 3)-alpha-D-glucosidic polysaccharides (yields, 82--90%) that constitute the backbone chains. The presence of small proportions of glycerol, erythritol, 1-O-alpha-D-glucosyl-D-glycerol, and also 2-O-alpha-D-glucosyl-D-erythritol in the products of Smith degradation suggests that the short side-chains are attached to the backbone chain by (1 leads to 4)-, (1 leads to 6)-, and (1 leads to 3)-alpha-D-glucosidic linkages.", "contents": "Structure of the water-insoluble alpha-D-glucan of Streptococcus salivarius HHT. Water-insoluble, non-adherent alpha-D-glucans have been obtained from Streptococcus salivarius HHT under two sets of conditions: from a growing culture, or synthesized enzymically by using a glucosyltransferase. In the former case, the glucan ([alpha]D + 197 degrees) was shown by methylation analysis to have a slightly branched structure containing a relatively high proportion (80%) of (1 leads to 3)-alpha-D-glucosidic linkages, together with small proportions of (1 leads to 6)- and (1 leads to 4)-alpha-D-glucosidic linkages. The enzymically synthesized glucan had a much less-branched structure, containing 88% of (1 leads to 3)-alpha-D-glucosidic linkages. Both glucans, on Smith degradation (sequential periodate oxidation, borohydride reduction, and mild acid hydrolysis), gave linear, (1 leads to 3)-alpha-D-glucosidic polysaccharides (yields, 82--90%) that constitute the backbone chains. The presence of small proportions of glycerol, erythritol, 1-O-alpha-D-glucosyl-D-glycerol, and also 2-O-alpha-D-glucosyl-D-erythritol in the products of Smith degradation suggests that the short side-chains are attached to the backbone chain by (1 leads to 4)-, (1 leads to 6)-, and (1 leads to 3)-alpha-D-glucosidic linkages."}
{"id": "PMID:487363", "title": "Structural studies on the capsular polysaccharide from Klebsiella serotype K64.", "content": "Partial hydrolysis with acid, methylation analysis (including uronic acid degradation), Smith degradation, and p.m.r. spectroscopy have been used to determine the primary structure of the capsular polysaccharide of Klebsiella serotype K64. The hexasaccharide repeating-unit, which also contains one O-acetyl substituent, comprises a 4)-alpha-D-GlcpA-(1 leads to 3)-alpha-D-Manp-(1 leads to 3)-beta-D-Glcp-(1 leads to 4)-alpha-D-Manp-(1 leads to chain with a 4,6-O-(1-carboxyethylidene)-beta-D-glucopyranosyl and an L-rhamnosyl group attached to the 4-linked D-mannosyl residue at 0-2 and 0-3, respectively.", "contents": "Structural studies on the capsular polysaccharide from Klebsiella serotype K64. Partial hydrolysis with acid, methylation analysis (including uronic acid degradation), Smith degradation, and p.m.r. spectroscopy have been used to determine the primary structure of the capsular polysaccharide of Klebsiella serotype K64. The hexasaccharide repeating-unit, which also contains one O-acetyl substituent, comprises a 4)-alpha-D-GlcpA-(1 leads to 3)-alpha-D-Manp-(1 leads to 3)-beta-D-Glcp-(1 leads to 4)-alpha-D-Manp-(1 leads to chain with a 4,6-O-(1-carboxyethylidene)-beta-D-glucopyranosyl and an L-rhamnosyl group attached to the 4-linked D-mannosyl residue at 0-2 and 0-3, respectively."}
{"id": "PMID:487364", "title": "A method for the determination of the chemical structure of glycolipids biosynthesized in vitro and the specificity and activity of glycosyltransferases.", "content": "A very sensitive method for the qualitative and quantitative investigation of the biosynthesis of the oligosaccharide chains of glycolipids in such tissues as cultured cells, biopsies, or cell organelles, is described. It permits the determination, on an ultramicro-scale, of the specificity of glycosyltransferases in terms of the exact chemical structure of the products that are synthesized from known labeled glycolipid precursors, and defines without ambiguity the precursor-to-product relationship in biosynthetic incubations in vitro.", "contents": "A method for the determination of the chemical structure of glycolipids biosynthesized in vitro and the specificity and activity of glycosyltransferases. A very sensitive method for the qualitative and quantitative investigation of the biosynthesis of the oligosaccharide chains of glycolipids in such tissues as cultured cells, biopsies, or cell organelles, is described. It permits the determination, on an ultramicro-scale, of the specificity of glycosyltransferases in terms of the exact chemical structure of the products that are synthesized from known labeled glycolipid precursors, and defines without ambiguity the precursor-to-product relationship in biosynthetic incubations in vitro."}
{"id": "PMID:487365", "title": "Spin-labelling of sialic acid in soluble and cell-surface glycoproteins.", "content": "A new, mild method is described for spin-labelling sialic acid residues in situ. The procedure involves the formation of C-1 sialamides and has been applied to a serum glycoprotein, a mucin, tissue sections from human colon, and erythrocyte membrane components. The selectivity of the method and its possible applicability to other types of labelling are discussed.", "contents": "Spin-labelling of sialic acid in soluble and cell-surface glycoproteins. A new, mild method is described for spin-labelling sialic acid residues in situ. The procedure involves the formation of C-1 sialamides and has been applied to a serum glycoprotein, a mucin, tissue sections from human colon, and erythrocyte membrane components. The selectivity of the method and its possible applicability to other types of labelling are discussed."}
{"id": "PMID:487366", "title": "Purification and characterisation of two endo-(1 linked to 3)-beta-D-glucanases from Telescopium telescopium.", "content": "The crystalline style of the gastropod Telescopium telescopium contains two (1 linked to 3)-beta-D-glucanases and a beta-D-glucosidase. The two glucanases (I and II) have been purified and shown to be endo-enzymes. Both enzymes attack laminarin, carboxymethylpachyman, and lichenin, but have no action towards carboxymethyl-cellulose. The main products of hydrolysis of laminarin are D-glucose and beta-(1 linked to 3)-linked oligosaccharides of d.p. 2, 3, and 4. Glucanases I and II are similar to each other, although they differ in molecular weight and kinetic properties.", "contents": "Purification and characterisation of two endo-(1 linked to 3)-beta-D-glucanases from Telescopium telescopium. The crystalline style of the gastropod Telescopium telescopium contains two (1 linked to 3)-beta-D-glucanases and a beta-D-glucosidase. The two glucanases (I and II) have been purified and shown to be endo-enzymes. Both enzymes attack laminarin, carboxymethylpachyman, and lichenin, but have no action towards carboxymethyl-cellulose. The main products of hydrolysis of laminarin are D-glucose and beta-(1 linked to 3)-linked oligosaccharides of d.p. 2, 3, and 4. Glucanases I and II are similar to each other, although they differ in molecular weight and kinetic properties."}
{"id": "PMID:487372", "title": "Radioactive particle imaging of coronary blood flow distribution.", "content": "The intracoronary injection of radionuclide-labeled macroaggregated albumin particles has been shown to be safe and to provide excellent myocardial imaging. When performed in the resting state, it is more sensitive than either the ECG or ventriculogram in identifying areas of scarring and fibrosis due to previous myocardial infarction. Combined with contrast induced coronary hyperemia, intracoronary MAA particles are useful in identifying stenoses of moderate severity that do not alter resting coronary blood flow. Particle studies are likely more sensitive than the rest-exercise 210T1 myocardial scan in identifying patients with significant coronary artery disease. Resting perfusion abnormalities are more closely related to improved or unimproved left ventricular function after surgery than are the ECG or resting left ventriculogram. While the long-range clinical utility of the particle technique is uncertain, its most useful application currently is identification of significant stenoses undetected by 201T1 and of questionable significance by arteriography.", "contents": "Radioactive particle imaging of coronary blood flow distribution. The intracoronary injection of radionuclide-labeled macroaggregated albumin particles has been shown to be safe and to provide excellent myocardial imaging. When performed in the resting state, it is more sensitive than either the ECG or ventriculogram in identifying areas of scarring and fibrosis due to previous myocardial infarction. Combined with contrast induced coronary hyperemia, intracoronary MAA particles are useful in identifying stenoses of moderate severity that do not alter resting coronary blood flow. Particle studies are likely more sensitive than the rest-exercise 210T1 myocardial scan in identifying patients with significant coronary artery disease. Resting perfusion abnormalities are more closely related to improved or unimproved left ventricular function after surgery than are the ECG or resting left ventriculogram. While the long-range clinical utility of the particle technique is uncertain, its most useful application currently is identification of significant stenoses undetected by 201T1 and of questionable significance by arteriography."}
{"id": "PMID:487375", "title": "Scintigraphic evaluation of left ventricular function.", "content": "Scintigraphic studies, performed with a scintillation camera and a small computer, can provide information concerning total and regional left ventricular function (Table 1). Of the two types of studies (first-pass and gated blood pool) the gated blood study is more appropriate for studies of regional function than is the first-pass method. Ejection fraction, an index of global ventricular function, is measureable from first-pass or gated blood pool studies. Gated blood pool studies allow visual assessment of net ventricular function as well as the local functional status of the myocardium. Multiple gated blood pool studies allow computation of ventricular volume curves, from which ejection fraction and ejection rate may be determined. There are several areas of application for these relatively inexpensive and virtually noninvasive studies. In patients with recent myocardial infarction, visual assessment of regional ventricular wall motion and quantitative measurement of ejection fraction provide additional prognostic information, especially when measured serially. In patients with coronary artery disease, the development of regional ventricular wall motion abnormalities may indicate local myocardial ischemia. Consequently, scintigraphic imaging may add significantly to the usefulness of exercise stress testing.", "contents": "Scintigraphic evaluation of left ventricular function. Scintigraphic studies, performed with a scintillation camera and a small computer, can provide information concerning total and regional left ventricular function (Table 1). Of the two types of studies (first-pass and gated blood pool) the gated blood study is more appropriate for studies of regional function than is the first-pass method. Ejection fraction, an index of global ventricular function, is measureable from first-pass or gated blood pool studies. Gated blood pool studies allow visual assessment of net ventricular function as well as the local functional status of the myocardium. Multiple gated blood pool studies allow computation of ventricular volume curves, from which ejection fraction and ejection rate may be determined. There are several areas of application for these relatively inexpensive and virtually noninvasive studies. In patients with recent myocardial infarction, visual assessment of regional ventricular wall motion and quantitative measurement of ejection fraction provide additional prognostic information, especially when measured serially. In patients with coronary artery disease, the development of regional ventricular wall motion abnormalities may indicate local myocardial ischemia. Consequently, scintigraphic imaging may add significantly to the usefulness of exercise stress testing."}
{"id": "PMID:487376", "title": "Physiology and physics of nuclear cardiology.", "content": "This chapter is a primer on the physics of radionuclide detection, flow physiology, and methods of in vivo evaluation of myocardial metabolism and intercavitary flow by noninvasive methods of intravenous isotope injection. This summary presents key concepts for the application of currently available instrumentation as well as future directions of nuclear cardiology. 1. Quantitative information is obtained in nuclear cardiology at the cost of high resolution imaging for two reasons: (a) the intrinsic resolution of the detecting systems is limited by available technology, and (b) the statistics required to achieve a high resolution image necessitate doses and imaging times far in excess of those which can be tolerated. Image resolution for both projection images as well as transverse sections are limited to the range of 5 to 20 mm, depending upon the configuration and instrument involved. 2. The second important concept is the fact that nuclear cardiology gives quantitative information regarding the amount of radiopharmaceutical which has accumulated in or is flowing through the cardiovascular system. This information allows one to deduce the dynamics of flow as well as actual metabolic rates. 3. The major emphasis for future work might well lie in the multiple transverse section imaging of the myocardium using both rotating and static devices. The key feature of this approach is the fact that the volume of interest can be localized and actual concentrations of radiopharmaceuticals can be measured by external detection using reconstruction tomography images. 4. Quantitative data on the distribution of a metabolite which accumulates in the myocardium is of little value if regional blood flow is not also known. 5. Finally, it is shown in this chapter that specific volume flow can be evaluated using short half-life isotopes and equations derived from the principle of conservation of mass. In principle it is now possible to obtain quantitative values delineating endo- and epicardial flow for the heart of man without invasive catheterization or high radiation doses. These procedures involve constant inhalation of carbon dioxide labeled with 15O which converts to labeled water and can be used for evaluating myocardial perfusion; bolus injection of 82Rb, a short half-life analogue of potassium, for repeated (every 5 min) imaging of the evolution of myocardial infarction size; evaluation of the accumulation of labeled fatty acids, amino acids, and sugars in the myocarium; presentation of images which reflect the magnitude of ejection fraction; and noninvasive evaluation of cardiac shunts. It is now possible to perform on the same patient during a few hours the following studies of myocardium: cation perfusion evaluation; water perfusion; uptake of fatty acids, amino acids, and glucose; oxygen utilization of the myocardium; and even measurement of the quantity of lung water. We now have the tools and methods to evaluate the in vivo biochemistry of the ischemic, 128 infarcting, repairing, and hypertrophic myocardium.", "contents": "Physiology and physics of nuclear cardiology. This chapter is a primer on the physics of radionuclide detection, flow physiology, and methods of in vivo evaluation of myocardial metabolism and intercavitary flow by noninvasive methods of intravenous isotope injection. This summary presents key concepts for the application of currently available instrumentation as well as future directions of nuclear cardiology. 1. Quantitative information is obtained in nuclear cardiology at the cost of high resolution imaging for two reasons: (a) the intrinsic resolution of the detecting systems is limited by available technology, and (b) the statistics required to achieve a high resolution image necessitate doses and imaging times far in excess of those which can be tolerated. Image resolution for both projection images as well as transverse sections are limited to the range of 5 to 20 mm, depending upon the configuration and instrument involved. 2. The second important concept is the fact that nuclear cardiology gives quantitative information regarding the amount of radiopharmaceutical which has accumulated in or is flowing through the cardiovascular system. This information allows one to deduce the dynamics of flow as well as actual metabolic rates. 3. The major emphasis for future work might well lie in the multiple transverse section imaging of the myocardium using both rotating and static devices. The key feature of this approach is the fact that the volume of interest can be localized and actual concentrations of radiopharmaceuticals can be measured by external detection using reconstruction tomography images. 4. Quantitative data on the distribution of a metabolite which accumulates in the myocardium is of little value if regional blood flow is not also known. 5. Finally, it is shown in this chapter that specific volume flow can be evaluated using short half-life isotopes and equations derived from the principle of conservation of mass. In principle it is now possible to obtain quantitative values delineating endo- and epicardial flow for the heart of man without invasive catheterization or high radiation doses. These procedures involve constant inhalation of carbon dioxide labeled with 15O which converts to labeled water and can be used for evaluating myocardial perfusion; bolus injection of 82Rb, a short half-life analogue of potassium, for repeated (every 5 min) imaging of the evolution of myocardial infarction size; evaluation of the accumulation of labeled fatty acids, amino acids, and sugars in the myocarium; presentation of images which reflect the magnitude of ejection fraction; and noninvasive evaluation of cardiac shunts. It is now possible to perform on the same patient during a few hours the following studies of myocardium: cation perfusion evaluation; water perfusion; uptake of fatty acids, amino acids, and glucose; oxygen utilization of the myocardium; and even measurement of the quantity of lung water. We now have the tools and methods to evaluate the in vivo biochemistry of the ischemic, 128 infarcting, repairing, and hypertrophic myocardium."}
{"id": "PMID:487378", "title": "The effect of myocardial force on coronary transmural flow distribution.", "content": "The role of myocardial force as a determinant of transmural coronary flow distribution was examined in the presence and absence of autoregulation by infusing calcium chloride into a segment of the left coronary system. The concentration of calcium used was adequate to increase force but did not alter coronary pressure or endoventricular pressure. No alterations in transmural gradients of flow were observed following increased regional force. These results suggest that increases in regional force are not of importance as a determinant of transmural blood flow.", "contents": "The effect of myocardial force on coronary transmural flow distribution. The role of myocardial force as a determinant of transmural coronary flow distribution was examined in the presence and absence of autoregulation by infusing calcium chloride into a segment of the left coronary system. The concentration of calcium used was adequate to increase force but did not alter coronary pressure or endoventricular pressure. No alterations in transmural gradients of flow were observed following increased regional force. These results suggest that increases in regional force are not of importance as a determinant of transmural blood flow."}
{"id": "PMID:487379", "title": "Acute voltage, charge, and energy thresholds as functions of electrode size for electrical stimulation of the canine heart.", "content": "This paper analyses the relationships between electrode size and charge, voltage, and energy thresholds in acute animal experiments. Cathodal stimuli of 1 ms duration are applied to canine hearts by using epicardial disc electrodes. Threshold charge in constant current and in constant voltage stimulation proves to be proportional to the electrode radius (a) to the power 1.5 for a greater than 0.4 mm and to be independent of electrode size for a less than 0.2 mm. Voltage and energy thresholds  are proportional to square root a and a2 respectively for a greater than 1 mm. Voltage thresholds show a minimum at a radius of about 0.5 mm, energy thresholds at about 0.3 mm. These results are explained by using two principles. The first is that the charge applied to the heart determines the response of the tissue to a stimulus and the second, that electrode impedance may be described by an RC-series circuit in these experiments. The resistance in this circuit is inversely proportional to electrode radius, in agreement with calculation of the electric field around the electrode. Electrode capacity depends linearly on electrode surface area. Stimulation is most efficient under the circumstances mentioned above for an electrode radius of about 0.3 mm.", "contents": "Acute voltage, charge, and energy thresholds as functions of electrode size for electrical stimulation of the canine heart. This paper analyses the relationships between electrode size and charge, voltage, and energy thresholds in acute animal experiments. Cathodal stimuli of 1 ms duration are applied to canine hearts by using epicardial disc electrodes. Threshold charge in constant current and in constant voltage stimulation proves to be proportional to the electrode radius (a) to the power 1.5 for a greater than 0.4 mm and to be independent of electrode size for a less than 0.2 mm. Voltage and energy thresholds  are proportional to square root a and a2 respectively for a greater than 1 mm. Voltage thresholds show a minimum at a radius of about 0.5 mm, energy thresholds at about 0.3 mm. These results are explained by using two principles. The first is that the charge applied to the heart determines the response of the tissue to a stimulus and the second, that electrode impedance may be described by an RC-series circuit in these experiments. The resistance in this circuit is inversely proportional to electrode radius, in agreement with calculation of the electric field around the electrode. Electrode capacity depends linearly on electrode surface area. Stimulation is most efficient under the circumstances mentioned above for an electrode radius of about 0.3 mm."}
{"id": "PMID:487380", "title": "Effect of increases in afterload before and after coronary occlusion in awake dogs.", "content": "Ventricular function curves relating stroke work and left ventricular end-diastolic pressure were generated in awake dogs during increases in preload produced by infusion of fluid and during increases in afterload produced by administration of phenylephrine. The ventricular function curves produced by preloading were steeply upsloping whereas those produced by afterloading were essentially horizontal. Coronary occlusion produced downward displacement of these horizontal curves, but no change in slope. This increases in afterload did not help to demonstrate the functional impairment produced by coronary occlusion.", "contents": "Effect of increases in afterload before and after coronary occlusion in awake dogs. Ventricular function curves relating stroke work and left ventricular end-diastolic pressure were generated in awake dogs during increases in preload produced by infusion of fluid and during increases in afterload produced by administration of phenylephrine. The ventricular function curves produced by preloading were steeply upsloping whereas those produced by afterloading were essentially horizontal. Coronary occlusion produced downward displacement of these horizontal curves, but no change in slope. This increases in afterload did not help to demonstrate the functional impairment produced by coronary occlusion."}
{"id": "PMID:487381", "title": "Myocardial anaerobic metabolism during isoprenaline infusion in a cyanotic animal model: possible cause of myocardial dysfunction in cyanotic congential heart disease.", "content": "Mongrel dogs were made acutely cyanotic by anastomosis of the inferior vena cava to the left atrium and measurements of high fidelity left ventricular pressure, arterial pressure, blood gases, and myocardial lactate arteriovenous differences were determined before pacing, with atrial pacing at a rate of approximately 240 per minute, and during an infusion of isoprenaline to increase myocardial oxygen demands. The animals were divided into two groups on the basis of the arterial PO2 during isoprenaline: acyanotic or control dogs (n = 4, PO2 greater than or equal to 6.7 kPa [50 mmHg], mean = 52) and cyanotic dogs (n = 5, PO2 less than or equal to 4.7 kPa (35 mmHg), mean = 32). Myocardial lactate arterio-venous difference showed no significant changes in the control dogs with pacing or isoprenaline, but myocardial lactate production occurred in all five cyanotic dogs with isoprenaline infusion. These studies demonstrate that myocardial anaerobic metabolism can be produced during times of stress with isoprenaline infusion in a cyanotic animal model at systemic oxygen levels that can occur clinically.", "contents": "Myocardial anaerobic metabolism during isoprenaline infusion in a cyanotic animal model: possible cause of myocardial dysfunction in cyanotic congential heart disease. Mongrel dogs were made acutely cyanotic by anastomosis of the inferior vena cava to the left atrium and measurements of high fidelity left ventricular pressure, arterial pressure, blood gases, and myocardial lactate arteriovenous differences were determined before pacing, with atrial pacing at a rate of approximately 240 per minute, and during an infusion of isoprenaline to increase myocardial oxygen demands. The animals were divided into two groups on the basis of the arterial PO2 during isoprenaline: acyanotic or control dogs (n = 4, PO2 greater than or equal to 6.7 kPa [50 mmHg], mean = 52) and cyanotic dogs (n = 5, PO2 less than or equal to 4.7 kPa (35 mmHg), mean = 32). Myocardial lactate arterio-venous difference showed no significant changes in the control dogs with pacing or isoprenaline, but myocardial lactate production occurred in all five cyanotic dogs with isoprenaline infusion. These studies demonstrate that myocardial anaerobic metabolism can be produced during times of stress with isoprenaline infusion in a cyanotic animal model at systemic oxygen levels that can occur clinically."}
{"id": "PMID:487382", "title": "Coronary artery pressure development and wave transmission characteristics in the horse.", "content": "Measurements of the pressure waveform development and the wave transmission characteristics in the left extramural coronary arteries of the horse have been carried out. Near the ostium the left coronary pressure waveforms are seen to be virtually identical to the corresponding aortic root waveforms; however, the present of low frequency, relatively large amplitude pressure oscillations (on the order of 5 to 10 Hz) gradually become the dominant diastolic feature as one proceeds distally from the left ostium, and these eventually completely mask the incisura. In a limited number of experiments, these oscillations have been simultaneously observed on both centerline velocity and phasic flow signals. These are felt to be primarily due to wave reflection phenomena, but may represent a combined effect of wave reflections and the natural oscillatory motion of the heart mass. Peak systolic pressures were found to remain relatively unchanged as one proceeds distally; however, the end-diastolic pressures were found to decrease steadily, thus giving rise to an increasing pulse pressure and a gradually decreasing mean pressure. Wave speeds in the coronary arteries were found to range between 4 and 11 m.s-1, and the data obtained indicate the wave speed to be highly dependent on both local intralumenal pressure and spatial location.", "contents": "Coronary artery pressure development and wave transmission characteristics in the horse. Measurements of the pressure waveform development and the wave transmission characteristics in the left extramural coronary arteries of the horse have been carried out. Near the ostium the left coronary pressure waveforms are seen to be virtually identical to the corresponding aortic root waveforms; however, the present of low frequency, relatively large amplitude pressure oscillations (on the order of 5 to 10 Hz) gradually become the dominant diastolic feature as one proceeds distally from the left ostium, and these eventually completely mask the incisura. In a limited number of experiments, these oscillations have been simultaneously observed on both centerline velocity and phasic flow signals. These are felt to be primarily due to wave reflection phenomena, but may represent a combined effect of wave reflections and the natural oscillatory motion of the heart mass. Peak systolic pressures were found to remain relatively unchanged as one proceeds distally; however, the end-diastolic pressures were found to decrease steadily, thus giving rise to an increasing pulse pressure and a gradually decreasing mean pressure. Wave speeds in the coronary arteries were found to range between 4 and 11 m.s-1, and the data obtained indicate the wave speed to be highly dependent on both local intralumenal pressure and spatial location."}
{"id": "PMID:487383", "title": "Isoprenaline-induced myocardial infarction in spontaneously hypertensive rats.", "content": "Spontaneously hypertensive rats were subjected to massive myocardial infarction. The pathophysiological changes were similar to those of normotensive rats but the spontaneously hypertensive rats developed congestive heart failure during myocardial repair rather than during the necrosis phase and survived in superior numbers. Blood pressure fell despite maximally increased corticosterone levels. Atrial and ventricular thromboses and left ventricular aneurysm formation were frequent.", "contents": "Isoprenaline-induced myocardial infarction in spontaneously hypertensive rats. Spontaneously hypertensive rats were subjected to massive myocardial infarction. The pathophysiological changes were similar to those of normotensive rats but the spontaneously hypertensive rats developed congestive heart failure during myocardial repair rather than during the necrosis phase and survived in superior numbers. Blood pressure fell despite maximally increased corticosterone levels. Atrial and ventricular thromboses and left ventricular aneurysm formation were frequent."}
{"id": "PMID:487384", "title": "Myocardial blood flow and O2-supply following dextran-haemodilution and methaemoglobinaemia in the dog.", "content": "The adequacy of oxygen-supply to the left ventricular myocardium was compared in anaesthetised dogs following: a) isovolaemic haemodilution with Dextran 70 (to haematocrit = 20 +/- 2%), b) the acute induction of methaemoglobinaemia (to methaemoglobin-concentration = 53 +/- 7%), bu subjecting them to an exchange-transfusion with methaemoglobin-containing erythrocytes in dextran) and c) a control exchange-transfusion with normal homologous erythrocytes in dextran. Myocardial oxygen-supply was assessed by the measurement of myocardial blood flow (using 15 +/- 5 micron microspheres) and of the PO2 in coronary sinus blood. In dextran-haemodiluted dogs, mean myocardial blood flow was 92% greater than in the control group, allowing the maintenance of normal coronary sinus PO2. In the methaemoglobinaemic group, flow was only 15% greater than in the control group; because oxygen-capacity was halved in this group, myocardial oxygen supply was impaired and coronary sinus PO2 fell. Infusions of isoproterenol (0.1 microgram . kg-1 . min-1) produced similar myocardial flow-increments in the haemodiluted and control groups with similar transmural distribution. In the methaemoglobinaemic group, however, the subendocardial flow-increment was reduced, in association with a further reduction in coronary sinus PO2 and an attenuated contractile response to the drug. These findings suggest that the rheological effects of haemodilution play an important role in the maintenance of myocardial oxygen-supply, but in significant methaemoglobinaemia, the oxygen supply of the myocardium may be severely curtailed.", "contents": "Myocardial blood flow and O2-supply following dextran-haemodilution and methaemoglobinaemia in the dog. The adequacy of oxygen-supply to the left ventricular myocardium was compared in anaesthetised dogs following: a) isovolaemic haemodilution with Dextran 70 (to haematocrit = 20 +/- 2%), b) the acute induction of methaemoglobinaemia (to methaemoglobin-concentration = 53 +/- 7%), bu subjecting them to an exchange-transfusion with methaemoglobin-containing erythrocytes in dextran) and c) a control exchange-transfusion with normal homologous erythrocytes in dextran. Myocardial oxygen-supply was assessed by the measurement of myocardial blood flow (using 15 +/- 5 micron microspheres) and of the PO2 in coronary sinus blood. In dextran-haemodiluted dogs, mean myocardial blood flow was 92% greater than in the control group, allowing the maintenance of normal coronary sinus PO2. In the methaemoglobinaemic group, flow was only 15% greater than in the control group; because oxygen-capacity was halved in this group, myocardial oxygen supply was impaired and coronary sinus PO2 fell. Infusions of isoproterenol (0.1 microgram . kg-1 . min-1) produced similar myocardial flow-increments in the haemodiluted and control groups with similar transmural distribution. In the methaemoglobinaemic group, however, the subendocardial flow-increment was reduced, in association with a further reduction in coronary sinus PO2 and an attenuated contractile response to the drug. These findings suggest that the rheological effects of haemodilution play an important role in the maintenance of myocardial oxygen-supply, but in significant methaemoglobinaemia, the oxygen supply of the myocardium may be severely curtailed."}
{"id": "PMID:487385", "title": "Pulmonary and systemic vascular responses to hypoxia after chemical sympathectomy.", "content": "Mongrel dogs were anesthetised and exposed to acute hypoxia (8% or 10% O2 for 10 min) before and again either 2 or 12 days after 6-hydroxydopamine (6-OHDA, 80 mg . kg-1) pretreatment. Under normoxic conditions, systemic arterial pressure and systemic vascular resistance were reduced 2 days post 6-OHDA, but only systemic resistance was reduced 12 days post 6-OHDA. Pulmonary vascular haemodynamics were unchanged by 6-OHDA under normoxic conditions. No changes in the cardiac output, pulmonary, and systemic haemodynamic or ventilatory responses to hypoxia were observed 2 days post 6-OHDA, although the heart rate response was reduced. Similarly, 12 days post 6-OHDA, reponses to hypoxia were unchanged except for a reduced systemic pressor response. A significant transient fall in systemic arterial pressure was observed at the start of the hypoxic exposure 2 days post 6-OHDA. The ventilatory responses to hypoxia appeared to be influenced very little by the sympathetic nervous system. The results indicate that pulmonary vascular resistance is under little sympathetic influence, and that hypoxic pulmonary vasoconstriction is not mediated by the sympathetic nervous system.", "contents": "Pulmonary and systemic vascular responses to hypoxia after chemical sympathectomy. Mongrel dogs were anesthetised and exposed to acute hypoxia (8% or 10% O2 for 10 min) before and again either 2 or 12 days after 6-hydroxydopamine (6-OHDA, 80 mg . kg-1) pretreatment. Under normoxic conditions, systemic arterial pressure and systemic vascular resistance were reduced 2 days post 6-OHDA, but only systemic resistance was reduced 12 days post 6-OHDA. Pulmonary vascular haemodynamics were unchanged by 6-OHDA under normoxic conditions. No changes in the cardiac output, pulmonary, and systemic haemodynamic or ventilatory responses to hypoxia were observed 2 days post 6-OHDA, although the heart rate response was reduced. Similarly, 12 days post 6-OHDA, reponses to hypoxia were unchanged except for a reduced systemic pressor response. A significant transient fall in systemic arterial pressure was observed at the start of the hypoxic exposure 2 days post 6-OHDA. The ventilatory responses to hypoxia appeared to be influenced very little by the sympathetic nervous system. The results indicate that pulmonary vascular resistance is under little sympathetic influence, and that hypoxic pulmonary vasoconstriction is not mediated by the sympathetic nervous system."}
{"id": "PMID:487386", "title": "Demonstration of dissimilar acute haemodynamic effects of ethanol and acetaldehyde.", "content": "To determine whether the acute cardiac depressant effects of ethanol could be attributed to its metabolite (acetaldehyde), either ethanol or acetaldehyde was intravenously infused into pentobarbital anaesthetised, closed-chest dogs. At a venous blood ethanol level of 199 +/- 43 (SE) mg . dl-1, ejection fraction had decreased from 35 +/- 2 to 30 +/-2%, P less than 0.05, max dP/dt/end-diastolic volume from 14.0 +/- 2.1 to 8.6 +/- 1.1 kPa . s-1 . cm-3 (105 +/- 16 to 65 +/- 8 mmHg . s-1 . cm-3), P less than 0.02, whereas end-diastolic volume (P less than 0.005), myocardial oxygen consumption (P less than 0.05) and coronary blood flow (P less than 0.005) had increased. Higher ethanol levels exaggerated these changes when peak arterial acetaldehyde was 20.2 +/- mumol . litre-1. By contrast, infusion of acetaldehyde to a peak blood level comparable with that produced by ethanol increased cardiac output from 2.4 +/- 0.2 to 2.8 +/- 0.2 litre-1 . min-1 P less than 0.01), coronary sinus oxygen saturation from 46 +/- 4 to 55 +/- 3% (P less than 0.25) and reduced systemic resistance from 8.0 +/- 0.7 to 6.3 +/- 0.5 kPa . litre-1 . min-1 (60 +/- 5 to 47 +/- 4 mmHg . litre-1 . min-1) (P less than 0.001). High dosage of acetaldehyde to a level of 129 +/- 23 mumol . litre-1 produced elevation of cardiac output (P less than 0.001), ejection fraction (P less than 0.01), coronary blood flow (P less than 0.02), whereas systemic resistance (P less than 0.001), heart rate (P less than 0.05) and myocardial oxygen consumption (P less than 0.05) decreased. Discontinuation of acetaldehyde infusion significantly reversed these changes. Max dP/dt/left ventricular end-diastolic volume and left ventricular end-diastolic volume were not significantly altered by acetaldehyde. Thus, ethanol depresses cardiac performance and increases myocardial oxygen consumption. By contrast, acetaldehyde at levels produced by ethanol metabolism improves cardiac performance, consequent to afterload reduction, and reduces myocardial oxygen consumption.", "contents": "Demonstration of dissimilar acute haemodynamic effects of ethanol and acetaldehyde. To determine whether the acute cardiac depressant effects of ethanol could be attributed to its metabolite (acetaldehyde), either ethanol or acetaldehyde was intravenously infused into pentobarbital anaesthetised, closed-chest dogs. At a venous blood ethanol level of 199 +/- 43 (SE) mg . dl-1, ejection fraction had decreased from 35 +/- 2 to 30 +/-2%, P less than 0.05, max dP/dt/end-diastolic volume from 14.0 +/- 2.1 to 8.6 +/- 1.1 kPa . s-1 . cm-3 (105 +/- 16 to 65 +/- 8 mmHg . s-1 . cm-3), P less than 0.02, whereas end-diastolic volume (P less than 0.005), myocardial oxygen consumption (P less than 0.05) and coronary blood flow (P less than 0.005) had increased. Higher ethanol levels exaggerated these changes when peak arterial acetaldehyde was 20.2 +/- mumol . litre-1. By contrast, infusion of acetaldehyde to a peak blood level comparable with that produced by ethanol increased cardiac output from 2.4 +/- 0.2 to 2.8 +/- 0.2 litre-1 . min-1 P less than 0.01), coronary sinus oxygen saturation from 46 +/- 4 to 55 +/- 3% (P less than 0.25) and reduced systemic resistance from 8.0 +/- 0.7 to 6.3 +/- 0.5 kPa . litre-1 . min-1 (60 +/- 5 to 47 +/- 4 mmHg . litre-1 . min-1) (P less than 0.001). High dosage of acetaldehyde to a level of 129 +/- 23 mumol . litre-1 produced elevation of cardiac output (P less than 0.001), ejection fraction (P less than 0.01), coronary blood flow (P less than 0.02), whereas systemic resistance (P less than 0.001), heart rate (P less than 0.05) and myocardial oxygen consumption (P less than 0.05) decreased. Discontinuation of acetaldehyde infusion significantly reversed these changes. Max dP/dt/left ventricular end-diastolic volume and left ventricular end-diastolic volume were not significantly altered by acetaldehyde. Thus, ethanol depresses cardiac performance and increases myocardial oxygen consumption. By contrast, acetaldehyde at levels produced by ethanol metabolism improves cardiac performance, consequent to afterload reduction, and reduces myocardial oxygen consumption."}
{"id": "PMID:487390", "title": "Studies of isolated and enriched rat antral mucosa gastrin cells.", "content": "A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to remove the epithelial cells. This technique resulted in a significant enrichment of G-cells (3-4 fold) since the surface epithelial cells and upper portions of the glands were discarded before the initial G-cell fraction was collected. These cells in suspension were then isolated from each other by gentle pipetting in a DNase containing solution and designated the crude preparation (CP). The G-cells were then purified further by separating the cells according to size by velocity sedimentation. The greatest concentration of G-cells (15-25%) was found in the fraction containing cells with diameters of 10 to 12 micrometer. The effectiveness of the technique was evaluated by counting G-cells as identified by electron microscopy and immunofluorescence and assessing gastrin activity by radioimmunoassay. All three methods indicated that cell separation by gravity velocity sedimentation enriched the G-cell population 15-20 fold over their concentration in the CP. The combined techniques of selective pronase digestion followed by gravity velocity sedimentation resulted in an isolated cell preparation containing a 50-100 fold increase of G-cells over their normal distribution in the intact gastric mucosa. Since these isolated G-cells retain features indicating viability, their usefulness for in vitro studies is suggested.", "contents": "Studies of isolated and enriched rat antral mucosa gastrin cells. A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to remove the epithelial cells. This technique resulted in a significant enrichment of G-cells (3-4 fold) since the surface epithelial cells and upper portions of the glands were discarded before the initial G-cell fraction was collected. These cells in suspension were then isolated from each other by gentle pipetting in a DNase containing solution and designated the crude preparation (CP). The G-cells were then purified further by separating the cells according to size by velocity sedimentation. The greatest concentration of G-cells (15-25%) was found in the fraction containing cells with diameters of 10 to 12 micrometer. The effectiveness of the technique was evaluated by counting G-cells as identified by electron microscopy and immunofluorescence and assessing gastrin activity by radioimmunoassay. All three methods indicated that cell separation by gravity velocity sedimentation enriched the G-cell population 15-20 fold over their concentration in the CP. The combined techniques of selective pronase digestion followed by gravity velocity sedimentation resulted in an isolated cell preparation containing a 50-100 fold increase of G-cells over their normal distribution in the intact gastric mucosa. Since these isolated G-cells retain features indicating viability, their usefulness for in vitro studies is suggested."}
{"id": "PMID:487391", "title": "Immunoreactive pancreatic polypeptide (PP) occurs in the central and peripheral nervous system: preliminary immunocytochemical observations.", "content": "Pancreatic polypeptide (PP) is a candidate hormone of unknown physiological significance. It is produced by a population of endocrine cells in the pancreas. In the present study a PP-like peptide was found to occur in the mammalian and avian central andperipheral nervous systems. Immunoreactive nerve fibres and nerve cell bodies were widely distributed in the brain. Dense accumulations of nerve fibres occurred in the following areas: nucleus accumbens, interstitial nucleus of the stria terminalis, para- and periventricular hypothalamic nuclei, and medical preoptic area. In addition, nerve fibres were regularly seen in cortical areas. Immunoreactive perikarya were observed in the following regions: cortex, nucleus accumbens, neostriatum and septum. In the gut, immunoreactive nerve fibers were distributed in the myenteric plexus, in smooth muscle, around blood vessels, and in the core of the villi. Immunoreactive perikarya occurred in the submucosal and myenteric plexus, suggesting that PP immunonal PP-like peptide could be demonstrated with an antiserum raised against avian PP, but not with those raised against bovine or human PP. Thus, neuronal PP is distinct from the PP that occurs in pancreatic endocrine cells.", "contents": "Immunoreactive pancreatic polypeptide (PP) occurs in the central and peripheral nervous system: preliminary immunocytochemical observations. Pancreatic polypeptide (PP) is a candidate hormone of unknown physiological significance. It is produced by a population of endocrine cells in the pancreas. In the present study a PP-like peptide was found to occur in the mammalian and avian central andperipheral nervous systems. Immunoreactive nerve fibres and nerve cell bodies were widely distributed in the brain. Dense accumulations of nerve fibres occurred in the following areas: nucleus accumbens, interstitial nucleus of the stria terminalis, para- and periventricular hypothalamic nuclei, and medical preoptic area. In addition, nerve fibres were regularly seen in cortical areas. Immunoreactive perikarya were observed in the following regions: cortex, nucleus accumbens, neostriatum and septum. In the gut, immunoreactive nerve fibers were distributed in the myenteric plexus, in smooth muscle, around blood vessels, and in the core of the villi. Immunoreactive perikarya occurred in the submucosal and myenteric plexus, suggesting that PP immunonal PP-like peptide could be demonstrated with an antiserum raised against avian PP, but not with those raised against bovine or human PP. Thus, neuronal PP is distinct from the PP that occurs in pancreatic endocrine cells."}
{"id": "PMID:487392", "title": "Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphora vomitoria (Diptera).", "content": "Immunocytochemical staining has shown that the median neurosecretory cells (MNC) of the brain of the blowfly, Calliphora vomitoria, contain an insulin-like material which cross reacts with antibodies to bovine insulin. There are 24-26 paraldehyde fuchsin-positive MNC of which only 6-8 show the specific insulin-like immunoreactivity.", "contents": "Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphora vomitoria (Diptera). Immunocytochemical staining has shown that the median neurosecretory cells (MNC) of the brain of the blowfly, Calliphora vomitoria, contain an insulin-like material which cross reacts with antibodies to bovine insulin. There are 24-26 paraldehyde fuchsin-positive MNC of which only 6-8 show the specific insulin-like immunoreactivity."}
{"id": "PMID:487393", "title": "Functional morphology of the midgut of Aedes aegypti L. (Insecta, Diptera) during blood digestion.", "content": "Morphometric analysis of the epithelial lining of the stomach of A. aegypti suggests that digestion of the first blood meal in the stomach of this species can be viewed as a series of phases that can be correlated with physiological data from the literature. In phase Ia (0-10 h after blood meal [abm]) the whorls of the rough endoplasmic reticulum unfold, the Golgi zones increase, and the basal labyrinth is enlarged. This coincides with processes of synthesis and secretion (e.g., peritrophic membrane, esterases and lipases) and transport by the stomach epithelium. In phase Ib (10-20 habm) the cellular parameters measured further increase, indicating high synthetic and secretory activities (e.g., digestive enzymes). In phase Ic (20-30 habm) cell structures involved in synthesis and secretion still exhibit high values coinciding with maximal activity of proteases in the gut. Enhanced surface area of microvilli, prominent lipid inclusions, and appearance of glycogen deposits in the gut epithelium suggest increased absorption, storage, and transport functions of the stomach cells. In phase II (30-36 habm) structural alteration points to a gradual shift from synthesis and secretion to absorption, partial storage, and transport of nutrients. In phase III (36-72 habm) the cellular apparatus is reduced concomitant with the ending of the digestive cycle. Lipid inclusions and glycogen deposits disappear from the stomach epithelium.", "contents": "Functional morphology of the midgut of Aedes aegypti L. (Insecta, Diptera) during blood digestion. Morphometric analysis of the epithelial lining of the stomach of A. aegypti suggests that digestion of the first blood meal in the stomach of this species can be viewed as a series of phases that can be correlated with physiological data from the literature. In phase Ia (0-10 h after blood meal [abm]) the whorls of the rough endoplasmic reticulum unfold, the Golgi zones increase, and the basal labyrinth is enlarged. This coincides with processes of synthesis and secretion (e.g., peritrophic membrane, esterases and lipases) and transport by the stomach epithelium. In phase Ib (10-20 habm) the cellular parameters measured further increase, indicating high synthetic and secretory activities (e.g., digestive enzymes). In phase Ic (20-30 habm) cell structures involved in synthesis and secretion still exhibit high values coinciding with maximal activity of proteases in the gut. Enhanced surface area of microvilli, prominent lipid inclusions, and appearance of glycogen deposits in the gut epithelium suggest increased absorption, storage, and transport functions of the stomach cells. In phase II (30-36 habm) structural alteration points to a gradual shift from synthesis and secretion to absorption, partial storage, and transport of nutrients. In phase III (36-72 habm) the cellular apparatus is reduced concomitant with the ending of the digestive cycle. Lipid inclusions and glycogen deposits disappear from the stomach epithelium."}
{"id": "PMID:487394", "title": "New observations on the innervation of striated ducts in submandibular glands of cats, including possible peptidergic nerves.", "content": "The presence of hypolemmal axons between striated duct cells in submandibular glands of cats has been established electron microscopically. Axons were found between \"light\" cells, between \"light\" and \"dark\" cells and between \"light\" and basal cells. Hypolemmal axons were observed most frequently in the junctional region between striated and intercalary ducts. They were often more common in younger animals. \"Dark\" cells with numerous processes sometimes appeared to have a special relationship with hypolemmal axons. Most of the hypolemmal axons in striated ducts contained characteristic agranular vesicles of the cholinergic type, about 40 nm in diameter; many of these axons also contained large dense cored vesicles of the peptidergic type, about 100 nm in diameter and possessing a more clear outer halo. No adrenergic axons have been observed beneath the basal lamina of striated ducts, even after use of 5-OHDA. The possibility that some of the hypolemmal axons in striated ducts are peptidergic and their possible functions are discussed. Apart from other activities these axons may have a role in supplying special trophic factors to the cells, helping them in their developmental specialisation and maintaining them in normal condition. An absence of such factors after parasympathetic decentralisation may be responsible for the dramatic atrophic changes in striated duct cells, especially since the atrophy in the gland is not solely due to an absence of acetylcholine activation.", "contents": "New observations on the innervation of striated ducts in submandibular glands of cats, including possible peptidergic nerves. The presence of hypolemmal axons between striated duct cells in submandibular glands of cats has been established electron microscopically. Axons were found between \"light\" cells, between \"light\" and \"dark\" cells and between \"light\" and basal cells. Hypolemmal axons were observed most frequently in the junctional region between striated and intercalary ducts. They were often more common in younger animals. \"Dark\" cells with numerous processes sometimes appeared to have a special relationship with hypolemmal axons. Most of the hypolemmal axons in striated ducts contained characteristic agranular vesicles of the cholinergic type, about 40 nm in diameter; many of these axons also contained large dense cored vesicles of the peptidergic type, about 100 nm in diameter and possessing a more clear outer halo. No adrenergic axons have been observed beneath the basal lamina of striated ducts, even after use of 5-OHDA. The possibility that some of the hypolemmal axons in striated ducts are peptidergic and their possible functions are discussed. Apart from other activities these axons may have a role in supplying special trophic factors to the cells, helping them in their developmental specialisation and maintaining them in normal condition. An absence of such factors after parasympathetic decentralisation may be responsible for the dramatic atrophic changes in striated duct cells, especially since the atrophy in the gland is not solely due to an absence of acetylcholine activation."}
{"id": "PMID:487395", "title": "Retention of hemopoiesis in tail vertebrae of newborn rats.", "content": "In newborn rats, the marrow cavity of tail vertebrae is hemopoietic and contains no adipose tissue. The latter develops soon after birth to replace the hemopoietic tissue within the nonexpansile volume of the marrow cavity. By transposing the tail into the warmer environment of the abdomen, hemopoiesis was retained, and the development of adipose tissue was prevented in the transposed segment, when the operation was done in preweanling but not in adult animals. Systemic stimuli of erythropoiesis (phlebotomy, induced hemolysis) acted synergistically with the temperature increment to retain hemopoiesis. The findings support the concept that adipose cells in the yellow marrow are relatively stable and once developed, they are not readily mobilized. The findings may also explain the discrepancies in the results obtained by rat tail transposition.", "contents": "Retention of hemopoiesis in tail vertebrae of newborn rats. In newborn rats, the marrow cavity of tail vertebrae is hemopoietic and contains no adipose tissue. The latter develops soon after birth to replace the hemopoietic tissue within the nonexpansile volume of the marrow cavity. By transposing the tail into the warmer environment of the abdomen, hemopoiesis was retained, and the development of adipose tissue was prevented in the transposed segment, when the operation was done in preweanling but not in adult animals. Systemic stimuli of erythropoiesis (phlebotomy, induced hemolysis) acted synergistically with the temperature increment to retain hemopoiesis. The findings support the concept that adipose cells in the yellow marrow are relatively stable and once developed, they are not readily mobilized. The findings may also explain the discrepancies in the results obtained by rat tail transposition."}
{"id": "PMID:487396", "title": "Immunocytochemical localization of vasotocin and mesotocin in the hypothalamus of lacertilian reptiles.", "content": "The hypothalamic magnocellular neurosecretory system of lizards was studied with the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique at the light microscopic level. It was shown that vasotocin and mesotocin are synthesized in separate neurons. The vasotocinergic as well as the mesotocinergic perikarya are of different sizes. Both cell types occur in close juxtaposition, but without a distinct pattern of distribution. The external zone of the lacertilian median eminence contains numerous immunoreactive vasotocinergic fibers and only few immunoreactive mesotocinergic fibers. The general organization of the hypothalamic magnocellular neurosecretory system of lizards, as revealed by immunocytochemistry, is essentially similar to that revealed with unspecific staining methods.", "contents": "Immunocytochemical localization of vasotocin and mesotocin in the hypothalamus of lacertilian reptiles. The hypothalamic magnocellular neurosecretory system of lizards was studied with the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique at the light microscopic level. It was shown that vasotocin and mesotocin are synthesized in separate neurons. The vasotocinergic as well as the mesotocinergic perikarya are of different sizes. Both cell types occur in close juxtaposition, but without a distinct pattern of distribution. The external zone of the lacertilian median eminence contains numerous immunoreactive vasotocinergic fibers and only few immunoreactive mesotocinergic fibers. The general organization of the hypothalamic magnocellular neurosecretory system of lizards, as revealed by immunocytochemistry, is essentially similar to that revealed with unspecific staining methods."}
{"id": "PMID:487397", "title": "Effect of cyproterone acetate, levonorgestrel and progesterone on adrenal glands and reproductive organs in the beagle bitch.", "content": "The effects of short-term (8 weeks) treatment with different doses of cyproterone acetate (CPA), levonorgestrel (LN) and progesterone (PRO) on the adrenal gland, ovary, uterus and vagina were studied in cycle-synchronised beagle bitches (first anoestrus). The same organs from non-treated primiparous beagle bitches at the 6th and 9th weeks of pregnancy were also included. In the animals treated with the highest doses of CPA (4.0 mg/kg/day orally) and PRO (42.5 mg/kg/day subcutaneously), as well as in pregnant bitches (9th week of pregnancy), a decrease in adrenal weight and cortex width and also an apparent loss of cells in the zona fasciculata and zona reticularis were observed. A marked increase in ovarian weight was recorded only in pregnant bitches (6th week of pregnancy). This was reflected by the presence of multiple well-developed corpora lutea. The ovaries of virgin control and progestagen-treated bitches revealed ovarian atrophy. Progestagen treatment caused marked stimulation of the uterus, resulting in dose-related oedematous and hyperplastic changes. Comparable findings were also observed during pregnancy. The vaginal epithelium of the progestagen-treated and pregnant bitches showed marked mucification as compared with control bitches. These structural responses indicate that progestagen treatment stimulates a pseudopregnancy-like condition in the adrenal glands, uterus and vagina of the beagle bitch.", "contents": "Effect of cyproterone acetate, levonorgestrel and progesterone on adrenal glands and reproductive organs in the beagle bitch. The effects of short-term (8 weeks) treatment with different doses of cyproterone acetate (CPA), levonorgestrel (LN) and progesterone (PRO) on the adrenal gland, ovary, uterus and vagina were studied in cycle-synchronised beagle bitches (first anoestrus). The same organs from non-treated primiparous beagle bitches at the 6th and 9th weeks of pregnancy were also included. In the animals treated with the highest doses of CPA (4.0 mg/kg/day orally) and PRO (42.5 mg/kg/day subcutaneously), as well as in pregnant bitches (9th week of pregnancy), a decrease in adrenal weight and cortex width and also an apparent loss of cells in the zona fasciculata and zona reticularis were observed. A marked increase in ovarian weight was recorded only in pregnant bitches (6th week of pregnancy). This was reflected by the presence of multiple well-developed corpora lutea. The ovaries of virgin control and progestagen-treated bitches revealed ovarian atrophy. Progestagen treatment caused marked stimulation of the uterus, resulting in dose-related oedematous and hyperplastic changes. Comparable findings were also observed during pregnancy. The vaginal epithelium of the progestagen-treated and pregnant bitches showed marked mucification as compared with control bitches. These structural responses indicate that progestagen treatment stimulates a pseudopregnancy-like condition in the adrenal glands, uterus and vagina of the beagle bitch."}
{"id": "PMID:487398", "title": "Freeze-fracture study of taste bud pores in the foliate papillae of the rabbit.", "content": "In freeze-fractured specimens of taste buds from the foliate papillae of rabbits, the intercellular spaces are separated from the pore of the taste bud by zonulae occludentes of the \"tight-type\". Below these tight junctions numerous desmosomes are found at irregular intervals. The epithelial cells adjacent to the pore are also joined by single strands of fusion. The microvilli arising from the neck of the type I cells have a high particle density. The microvilli of type II cells and especially the short microvilli of peripherally situated cells have a lower intramembranous particle density. The single microvillus of type III cells has a very large diameter and is longer than the other microvilli. It contains a few larger intramembranous particles and vesicle-like protrusions of the membrane facing the cytoplasm. Transverse fracturing reveals a filamentous fine structure in all microvilli. The physiological implications of these observations are discussed.", "contents": "Freeze-fracture study of taste bud pores in the foliate papillae of the rabbit. In freeze-fractured specimens of taste buds from the foliate papillae of rabbits, the intercellular spaces are separated from the pore of the taste bud by zonulae occludentes of the \"tight-type\". Below these tight junctions numerous desmosomes are found at irregular intervals. The epithelial cells adjacent to the pore are also joined by single strands of fusion. The microvilli arising from the neck of the type I cells have a high particle density. The microvilli of type II cells and especially the short microvilli of peripherally situated cells have a lower intramembranous particle density. The single microvillus of type III cells has a very large diameter and is longer than the other microvilli. It contains a few larger intramembranous particles and vesicle-like protrusions of the membrane facing the cytoplasm. Transverse fracturing reveals a filamentous fine structure in all microvilli. The physiological implications of these observations are discussed."}
{"id": "PMID:487399", "title": "A stereological ultrastructural study of stimulated peritoneal macrophages in the germ-free mouse.", "content": "Peritoneal macrophage ultrastructure was analysed stereologically in germ-free mice given a single intraperitoneal injection of sterile, pyrogen-free saline. Thus the stimulant was non-particulate, non-antigenic and inorganic, and effects of immune reactions were minimal. Macrophages were recovered 1, 6, 24 and 72 h after stimulation. A sequence of structural alterations is reported which may be fundamental to macrophage activation. The plasma membrane and nuclear envelope increased in area within only 1 h of saline injection. During the next 5 h loss of plasma membrane, probably by pinocytosis, caused cellular \"rounding\" and clear-cut alteration in surface configuration. At the same time lysosome-like granules enlarged but decreased in number. By 24 h most cellular structures and compartments (including the plasma membrane) were enlarged. Morphological evidence of nuclear activation accompanied a rather modest enlargement of the nucleus at this stage. The RER hypertrophied last and must, therefore, be judged sufficient in resident macrophages to support the initial growth response which results after stimulation. Thus hypertrophy was observed eventually in every structure examined. Even the minimally activated macrophages resident in the peritoneum of germ-free mice respond readily to stimulation.", "contents": "A stereological ultrastructural study of stimulated peritoneal macrophages in the germ-free mouse. Peritoneal macrophage ultrastructure was analysed stereologically in germ-free mice given a single intraperitoneal injection of sterile, pyrogen-free saline. Thus the stimulant was non-particulate, non-antigenic and inorganic, and effects of immune reactions were minimal. Macrophages were recovered 1, 6, 24 and 72 h after stimulation. A sequence of structural alterations is reported which may be fundamental to macrophage activation. The plasma membrane and nuclear envelope increased in area within only 1 h of saline injection. During the next 5 h loss of plasma membrane, probably by pinocytosis, caused cellular \"rounding\" and clear-cut alteration in surface configuration. At the same time lysosome-like granules enlarged but decreased in number. By 24 h most cellular structures and compartments (including the plasma membrane) were enlarged. Morphological evidence of nuclear activation accompanied a rather modest enlargement of the nucleus at this stage. The RER hypertrophied last and must, therefore, be judged sufficient in resident macrophages to support the initial growth response which results after stimulation. Thus hypertrophy was observed eventually in every structure examined. Even the minimally activated macrophages resident in the peritoneum of germ-free mice respond readily to stimulation."}
{"id": "PMID:487400", "title": "Autoradiographic localization of 3H-glucocorticoids and 3H-cortexolone in mouse pituitary.", "content": "Autoradiograms of mouse pituitaries were prepared 30 min after injection of 3H-dexamethasone (3HDM), 3H-corticosterone (3HB) and 3H-cortexolone (3HS) either alone or in the presence of competing unlabelled steroids. 3H-dexamethasone accumulated in cell nuclei of both the pars distalis and the pars nervosa but not in those of the pars intermedia. This preferential accumulation (nuclear/cytoplasmic grain density, 4 : 1) was abolished by the concurrent administration of excess dexamethasone. 3H-corticosterone, to a much less marked extent than 3H-dexamethasone, accumulated in cell nuclei of the pars distalis but not in those of the pars intermedia and the pars nervosa. Excess unlabelled corticosterone diminished nuclear grain density in the pars distalis. After 3-h-cortexolone injection, preferential nuclear uptake was not observed. In a second series of experiments, excess dexamethasone (10 x, 100 x), corticosterone (100 x, 300 x) and cortexolone (100 x, 300 x) administered with 3H-dexamethasone were without effect on cytoplasmic grain density but totally abolished preferential nuclear accumulation. Parallel biochemical studies on kidney cytoplasmic preparations from the same animals showed no differences in total cytoplasmic radioactivity between treatments but marked differences in cytoplasmic bound 3H-dexamethasone. The results demonstrate: i) that dexamethasone binds specifically to cell nuclei of the pars distalis and the pars nervosa and that this nuclear concentration is abolished by competing corticosterone and cortexolone as well as dexamethasone; ii) that corticosterone localizes in cell nuclei of the pars distalis but much less markedly than dexamethasone; iii) that cortexolone fullfils the criteria of a glucocorticoid antagonist at the pituitary cell level.", "contents": "Autoradiographic localization of 3H-glucocorticoids and 3H-cortexolone in mouse pituitary. Autoradiograms of mouse pituitaries were prepared 30 min after injection of 3H-dexamethasone (3HDM), 3H-corticosterone (3HB) and 3H-cortexolone (3HS) either alone or in the presence of competing unlabelled steroids. 3H-dexamethasone accumulated in cell nuclei of both the pars distalis and the pars nervosa but not in those of the pars intermedia. This preferential accumulation (nuclear/cytoplasmic grain density, 4 : 1) was abolished by the concurrent administration of excess dexamethasone. 3H-corticosterone, to a much less marked extent than 3H-dexamethasone, accumulated in cell nuclei of the pars distalis but not in those of the pars intermedia and the pars nervosa. Excess unlabelled corticosterone diminished nuclear grain density in the pars distalis. After 3-h-cortexolone injection, preferential nuclear uptake was not observed. In a second series of experiments, excess dexamethasone (10 x, 100 x), corticosterone (100 x, 300 x) and cortexolone (100 x, 300 x) administered with 3H-dexamethasone were without effect on cytoplasmic grain density but totally abolished preferential nuclear accumulation. Parallel biochemical studies on kidney cytoplasmic preparations from the same animals showed no differences in total cytoplasmic radioactivity between treatments but marked differences in cytoplasmic bound 3H-dexamethasone. The results demonstrate: i) that dexamethasone binds specifically to cell nuclei of the pars distalis and the pars nervosa and that this nuclear concentration is abolished by competing corticosterone and cortexolone as well as dexamethasone; ii) that corticosterone localizes in cell nuclei of the pars distalis but much less markedly than dexamethasone; iii) that cortexolone fullfils the criteria of a glucocorticoid antagonist at the pituitary cell level."}
{"id": "PMID:487401", "title": "The subcommissural organ of Lacerta s. sicula Raf.: functional studies.", "content": "The secretory activity of the subcommissural organ (SCO) is affected by adrenalectomy, adrenalectomy + castration, and by an increase in ambient temperature in adrenalectomized and adrenalectomized + castrated animals. Adrenalectomy inhibits the activity of the SCO. After adrenalectomy + castration the decrease in the secretory activity of the SCO is more rapid. In contrast, an increase in the ambient temperature in adrenalectomized animals induces a recrudescence of the activity of the SCO. The increase in temperature in castrated + adrenalectomized lizards does not affect the inhibition produced by this type of surgical treatment. The histological changes are discussed on the basis of results obtained in the present study and in previous experiments.", "contents": "The subcommissural organ of Lacerta s. sicula Raf.: functional studies. The secretory activity of the subcommissural organ (SCO) is affected by adrenalectomy, adrenalectomy + castration, and by an increase in ambient temperature in adrenalectomized and adrenalectomized + castrated animals. Adrenalectomy inhibits the activity of the SCO. After adrenalectomy + castration the decrease in the secretory activity of the SCO is more rapid. In contrast, an increase in the ambient temperature in adrenalectomized animals induces a recrudescence of the activity of the SCO. The increase in temperature in castrated + adrenalectomized lizards does not affect the inhibition produced by this type of surgical treatment. The histological changes are discussed on the basis of results obtained in the present study and in previous experiments."}
{"id": "PMID:487402", "title": "Are there specialized junctions in the pars maculata of the distal tubule?", "content": "In the present study the tight junctions at the macula densa were compared to those of the adjacent straight and convoluted segments of the distal tubule using freeze fracturing and thin sectioning techniques. Only insignificant differences were found in the number of strands and the apico-basal depth of the tight junctions in the three distal tubular segments of rat, dog and tree shrew. In experiments with horseradish peroxidase on mice and tree shrews, the tracer did not penetrate the apical junctions in any of the distal tubular segments. Our findings do not support the concept of considerably higher permeability of the tight junctions at the macula densa, as previously reported. Gap junctions were never observed in the distal nephron. The present results suggest that the glomerulo-tubular feedback is more likely to be mediated by transcellular resorption of solutes than by passive diffusion through a leaky paracellular shunt pathway.", "contents": "Are there specialized junctions in the pars maculata of the distal tubule? In the present study the tight junctions at the macula densa were compared to those of the adjacent straight and convoluted segments of the distal tubule using freeze fracturing and thin sectioning techniques. Only insignificant differences were found in the number of strands and the apico-basal depth of the tight junctions in the three distal tubular segments of rat, dog and tree shrew. In experiments with horseradish peroxidase on mice and tree shrews, the tracer did not penetrate the apical junctions in any of the distal tubular segments. Our findings do not support the concept of considerably higher permeability of the tight junctions at the macula densa, as previously reported. Gap junctions were never observed in the distal nephron. The present results suggest that the glomerulo-tubular feedback is more likely to be mediated by transcellular resorption of solutes than by passive diffusion through a leaky paracellular shunt pathway."}
{"id": "PMID:487403", "title": "The first optic ganglion of the bee. III. Regional comparison of the morphology of photoreceptor-cell axons.", "content": "The nine receptor cells in each ommatidium of the worker bee end as six short visual fibres in the lamina and as three long visual fibres in the medulla. Behavioural and physiological evidence for regional variation in spectral sensitivity prompted observations on the morphology of the visual units. The distribution, branching pattern, diameter and the arrangement of axonal protusions of the characteristic receptor-cell axons were studied in various regions of the lamina. The six short visual fibres and two of the long visual fibres in each laminar cartridge are uniform over the total eye surface. Only the receptor axons of the ninth cell a UV and polarised light-sensitive cell, show obvious regional variation. In view of the regional constancy in morphology of eight of the nine receptor-cell axons, the regional variations in spectral sensitivity demand either functional subdivision of morphologically indistinguishable photoreceptors (e.g., content of different visual pigments) or a highly complex connectivity pattern of their axons in the first optic ganglion.", "contents": "The first optic ganglion of the bee. III. Regional comparison of the morphology of photoreceptor-cell axons. The nine receptor cells in each ommatidium of the worker bee end as six short visual fibres in the lamina and as three long visual fibres in the medulla. Behavioural and physiological evidence for regional variation in spectral sensitivity prompted observations on the morphology of the visual units. The distribution, branching pattern, diameter and the arrangement of axonal protusions of the characteristic receptor-cell axons were studied in various regions of the lamina. The six short visual fibres and two of the long visual fibres in each laminar cartridge are uniform over the total eye surface. Only the receptor axons of the ninth cell a UV and polarised light-sensitive cell, show obvious regional variation. In view of the regional constancy in morphology of eight of the nine receptor-cell axons, the regional variations in spectral sensitivity demand either functional subdivision of morphologically indistinguishable photoreceptors (e.g., content of different visual pigments) or a highly complex connectivity pattern of their axons in the first optic ganglion."}
{"id": "PMID:487404", "title": "Localization of pancreatic polypeptide cells in a limited lobe of the human neonate pancreas: remnant of the ventral primordium?", "content": "The localization of pancreatic polypeptide (PP) cells was studied in the pancreas of four human neonates by specific immunocytochemical techniques. PP cells were detected in all parts of the pancreas. However, examination at low magnification showed that they were considerably more numerous in a small lobe, located at the posterior-inferior part of the head region. It is suggested that this lobe corresponds to the part of the pancreas that is derived from the ventral primordium. Both in the lobe rich in PP cells and in the remainder of the pancreas, approximately 75% of PP cells were present in the islets and 25% distributed among acini and ducts.", "contents": "Localization of pancreatic polypeptide cells in a limited lobe of the human neonate pancreas: remnant of the ventral primordium? The localization of pancreatic polypeptide (PP) cells was studied in the pancreas of four human neonates by specific immunocytochemical techniques. PP cells were detected in all parts of the pancreas. However, examination at low magnification showed that they were considerably more numerous in a small lobe, located at the posterior-inferior part of the head region. It is suggested that this lobe corresponds to the part of the pancreas that is derived from the ventral primordium. Both in the lobe rich in PP cells and in the remainder of the pancreas, approximately 75% of PP cells were present in the islets and 25% distributed among acini and ducts."}
{"id": "PMID:487405", "title": "Fine structure of the receptor-free epithelium in the vomeronasal organ of the rat.", "content": "The topography and distribution of the epithelia of the vomeronasal organ were investigated in the adult male rat. Special attention was given to the light and transmission electron microscopic structure of the area that is known to be free of receptor cells. In contrast to several brief descriptions in which many cell types have been reported, only one cell type is presently described in different functional phases. In the rat, this epithelium does not show the typical features of a respiratory or, in more general terms, non-sensory epithelium. The authors propose to call this epithelium as \"receptor-free\" rather than respiratory, non-sensory or ciliated. The importance of the vomeronasal organ in the maintenance of olfacto-endocrine mechanisms and reproductive behavior is discussed.", "contents": "Fine structure of the receptor-free epithelium in the vomeronasal organ of the rat. The topography and distribution of the epithelia of the vomeronasal organ were investigated in the adult male rat. Special attention was given to the light and transmission electron microscopic structure of the area that is known to be free of receptor cells. In contrast to several brief descriptions in which many cell types have been reported, only one cell type is presently described in different functional phases. In the rat, this epithelium does not show the typical features of a respiratory or, in more general terms, non-sensory epithelium. The authors propose to call this epithelium as \"receptor-free\" rather than respiratory, non-sensory or ciliated. The importance of the vomeronasal organ in the maintenance of olfacto-endocrine mechanisms and reproductive behavior is discussed."}
{"id": "PMID:487406", "title": "Ultrastructural investigation on the cell membranes of the vomeronasal organ in the rat: a freeze-etching study.", "content": "The free surfaces and cell contacts in the epithelia of the vomeronasal organ of the rat were investigated by freeze-etching. The microvilli of receptor cells show a lower density of intramembranous particles (IMP) than the microvilli in the receptor-free epithelium. The ratio between the IMP on P- and E-face is approximately 11 : 1 in the receptor terminals, and 3.5 : 1 in the cilia and microvilli of the receptor-free epithelium. Although atypical in length and only poorly equipped with rootlet fibers, the cilia of the receptor-free epithelium are furnished with typical ciliary necklace structures of up to 10 rows of membrane particles. Differences in the density of IMP on the P-faces of different cilia are probably due to continual ciliogenesis and also due to the different age of cilia in the receptor-free epithelium. Zonulae occludentes show different configurations in the neuroepithelium and in the receptor-free epithelium. In the former, they show a tendency to cross-link and form facet-like patterns, reflecting a constant morphology and relative stability for this apical region. In the receptor-free epithelium the junctional rows of zonulae occludentes display only loosely interconnected networks and a tendency to orient parallel to each other and to the free surface. In addition to zonulae occludentes, typical square aggregations of IMP are observed in the receptor-free epithelium. They are not exclusively restricted to the zone of intensive cell contacts by means of fine interdigitating cell processes, and their function has yet to be identified experimentally.", "contents": "Ultrastructural investigation on the cell membranes of the vomeronasal organ in the rat: a freeze-etching study. The free surfaces and cell contacts in the epithelia of the vomeronasal organ of the rat were investigated by freeze-etching. The microvilli of receptor cells show a lower density of intramembranous particles (IMP) than the microvilli in the receptor-free epithelium. The ratio between the IMP on P- and E-face is approximately 11 : 1 in the receptor terminals, and 3.5 : 1 in the cilia and microvilli of the receptor-free epithelium. Although atypical in length and only poorly equipped with rootlet fibers, the cilia of the receptor-free epithelium are furnished with typical ciliary necklace structures of up to 10 rows of membrane particles. Differences in the density of IMP on the P-faces of different cilia are probably due to continual ciliogenesis and also due to the different age of cilia in the receptor-free epithelium. Zonulae occludentes show different configurations in the neuroepithelium and in the receptor-free epithelium. In the former, they show a tendency to cross-link and form facet-like patterns, reflecting a constant morphology and relative stability for this apical region. In the receptor-free epithelium the junctional rows of zonulae occludentes display only loosely interconnected networks and a tendency to orient parallel to each other and to the free surface. In addition to zonulae occludentes, typical square aggregations of IMP are observed in the receptor-free epithelium. They are not exclusively restricted to the zone of intensive cell contacts by means of fine interdigitating cell processes, and their function has yet to be identified experimentally."}
{"id": "PMID:487407", "title": "Classification of human placental villi. I. Histology.", "content": "The classification of human placental villi was reviewed on the basis of material prepared by means of special methods. The material from in situ normal-term placentae was biopsied by aspiration into glutaraldehyde. The classification was made on the basis of light-microscopic observations of semithin sections, reconstructions from serial sections, and scanning-electron micrographs. The peripheral villous tree is roughly divided into stem (ramuli), intermediate and terminal villi. The intermediate villi may be further subdivided as mature and immature types, which are found between the stem and terminal villi. Some of the terminal villi possess a local specialization described as the neck region. The histological characteristics and the branching pattern of each type are described, and the basis of the proposed classification is discussed.", "contents": "Classification of human placental villi. I. Histology. The classification of human placental villi was reviewed on the basis of material prepared by means of special methods. The material from in situ normal-term placentae was biopsied by aspiration into glutaraldehyde. The classification was made on the basis of light-microscopic observations of semithin sections, reconstructions from serial sections, and scanning-electron micrographs. The peripheral villous tree is roughly divided into stem (ramuli), intermediate and terminal villi. The intermediate villi may be further subdivided as mature and immature types, which are found between the stem and terminal villi. Some of the terminal villi possess a local specialization described as the neck region. The histological characteristics and the branching pattern of each type are described, and the basis of the proposed classification is discussed."}
{"id": "PMID:487408", "title": "Classification of human placental villi. II. Morphometry.", "content": "Villi of placental tissue obtained from normal term placentae at Caesarean section were embedded in Epon. Semithin sections were subjected to morphometry. The villi were first grouped according to the modified classification proposed in the foregoing paper. The parameters examined include villous numbers, size, vascularity, volume of trophoblast and connective tissue. The measured values differ markedly from those obtained from paraffin sections. Statistically significant differences exist between the different types of villi in various parameters, providing further evidence supporting the validity of the structural classification suggested.", "contents": "Classification of human placental villi. II. Morphometry. Villi of placental tissue obtained from normal term placentae at Caesarean section were embedded in Epon. Semithin sections were subjected to morphometry. The villi were first grouped according to the modified classification proposed in the foregoing paper. The parameters examined include villous numbers, size, vascularity, volume of trophoblast and connective tissue. The measured values differ markedly from those obtained from paraffin sections. Statistically significant differences exist between the different types of villi in various parameters, providing further evidence supporting the validity of the structural classification suggested."}
{"id": "PMID:487409", "title": "Demonstration of acid phosphatase activity induced by 20-hydroxyecdysone in the fat body of Calliphora.", "content": "In the larval fat body of Calliphora erythrocephala, protein accumulation and autophagic activity occur prior to the onset of puparium formation. The involvement of the lysosomal system in the degradation of sequestered protein and cell organelles can be demonstrated by the electron-microscopical cytochemical localization of the lysosomal marker enzyme acid phosphatase in so-called protein granules. These granules contain not only newly synthetized or absorbed protein but also remnants of cell organelles such as mitochondria and endoplasmic reticulum. Ligation of the larvae behind the brain-ring gland complex prevents the appearance of these acid phosphatase-positive granules. They can be induced in ligated larvae by the injection of 20-hydroxyecdysone into the abdomen. These findings are briefly discussed in relation to the role of moulting hormones in normal development, especially with regard to the induction of autophagic activity.", "contents": "Demonstration of acid phosphatase activity induced by 20-hydroxyecdysone in the fat body of Calliphora. In the larval fat body of Calliphora erythrocephala, protein accumulation and autophagic activity occur prior to the onset of puparium formation. The involvement of the lysosomal system in the degradation of sequestered protein and cell organelles can be demonstrated by the electron-microscopical cytochemical localization of the lysosomal marker enzyme acid phosphatase in so-called protein granules. These granules contain not only newly synthetized or absorbed protein but also remnants of cell organelles such as mitochondria and endoplasmic reticulum. Ligation of the larvae behind the brain-ring gland complex prevents the appearance of these acid phosphatase-positive granules. They can be induced in ligated larvae by the injection of 20-hydroxyecdysone into the abdomen. These findings are briefly discussed in relation to the role of moulting hormones in normal development, especially with regard to the induction of autophagic activity."}
{"id": "PMID:487410", "title": "Separation of non-proliferating from proliferating thymic lymphocytes based on light scatter analysis.", "content": "Two populations of thymic lymphocytes from eight-week-old mice were separated based on the light scatter intensity they generated while passing through a flow cell analyser. The two populations were subsequently studied morphologically and analysed for the relative amount of DNA present in each cell. Cells having a low light scatter intensity had a high nuclear:cytoplasmic ratio, a heterochromatic nucleus, and a proliferative index (S + G2 + M) of only 23%. Cells with a high light scatter intensity had a low nuclear:cytoplasmic ratio, dispersed chromatin, frequently a uropod with budding microvilli and a proliferative index of 71%. The low light scatter cells resemble small cortical thymocytes or lymphocytes from the thoracic duct described by others. The highest light scatter cells resemble medium lymphocytes shown by others to be proliferative.", "contents": "Separation of non-proliferating from proliferating thymic lymphocytes based on light scatter analysis. Two populations of thymic lymphocytes from eight-week-old mice were separated based on the light scatter intensity they generated while passing through a flow cell analyser. The two populations were subsequently studied morphologically and analysed for the relative amount of DNA present in each cell. Cells having a low light scatter intensity had a high nuclear:cytoplasmic ratio, a heterochromatic nucleus, and a proliferative index (S + G2 + M) of only 23%. Cells with a high light scatter intensity had a low nuclear:cytoplasmic ratio, dispersed chromatin, frequently a uropod with budding microvilli and a proliferative index of 71%. The low light scatter cells resemble small cortical thymocytes or lymphocytes from the thoracic duct described by others. The highest light scatter cells resemble medium lymphocytes shown by others to be proliferative."}
{"id": "PMID:487411", "title": "Larval adhesive organs and metamorphosis in ascidians. II. The mechanism of eversion of the papillae of Distaplia occidentalis.", "content": "The cup-shaped adhesive papillae of Distaplia occidentalis evert at the onset of metamorphosis and each transforms into a hyperboloidal configuration. The rate of transformation is a function of temperature. At 14 degrees C complete eversion takes about 30 seconds. Myoepithelial cells that extend from the rim to the base on the cup contract. Simultaneously the central part of the papilla advances 60--70 micrometers. During the last phases of eversion, collocytes (cells that secrete adhesives) on the inner wall of the cup and on the sides of the axial protrusion flow outward and form a collar-like structure. The myoepithelial cells contain arrays of thick and thin filaments. These become compacted during contraction. The surfaces of these cells become extensively folded as they shorten to about 1/3 of rest length. According to the proposed model the myoepithelial cells are the driving force in papillary eversion. Immediately after eversion is completed the papillae begin to retract. Eversion of the papillae is not inhibited by cytochalasin B, but the process of retraction is reversibly inhibited. Some histological characteristics of five types of everting papillae in four families of ascidians are compared.", "contents": "Larval adhesive organs and metamorphosis in ascidians. II. The mechanism of eversion of the papillae of Distaplia occidentalis. The cup-shaped adhesive papillae of Distaplia occidentalis evert at the onset of metamorphosis and each transforms into a hyperboloidal configuration. The rate of transformation is a function of temperature. At 14 degrees C complete eversion takes about 30 seconds. Myoepithelial cells that extend from the rim to the base on the cup contract. Simultaneously the central part of the papilla advances 60--70 micrometers. During the last phases of eversion, collocytes (cells that secrete adhesives) on the inner wall of the cup and on the sides of the axial protrusion flow outward and form a collar-like structure. The myoepithelial cells contain arrays of thick and thin filaments. These become compacted during contraction. The surfaces of these cells become extensively folded as they shorten to about 1/3 of rest length. According to the proposed model the myoepithelial cells are the driving force in papillary eversion. Immediately after eversion is completed the papillae begin to retract. Eversion of the papillae is not inhibited by cytochalasin B, but the process of retraction is reversibly inhibited. Some histological characteristics of five types of everting papillae in four families of ascidians are compared."}
{"id": "PMID:487412", "title": "An electron microscopic study of lipid absorption in the pyloric caeca of rainbow trout (Salmo gairdnerii) fed wax ester--rich zooplankton.", "content": "Rainbow trout were killed 4 and 18 h after being fed wax ester-rich marine zooplankton and the absorptive epithelium of the pyloric caeca examined by electron microscopy. Numerous osmiophilic drops were seen in the lamina propria underlying the epithelium of fish killed at both times, but these drops were only abundant within columnar epithelial cells of fish killed 4 h after feeding. Pinocytotic profiles were not common at the luminal plasma membranes, nor were osmiophilic droplets seen in the terminal web area between the luminal plasma membrane and the extensive smooth endoplasmic reticulum. Numerous osmiophilic droplets, 30--100 nm in diameter, were present in the cisternae of the smooth endoplasmic reticulum with up to five separate droplets per individual cisterna. Columnar epithelial cells also contained up to 100 large osmiophilic drops (\"conglomerates\") which tended to be concentrated in the supranuclear (Golgi) regions. The conglomerates were 250--1200 nm in diameter and were themselves made up of smaller droplets 30--400 nm in diameter. Conglomerates were present both within intracellular membranes and free in the cytoplasm. Osmiophilic droplets in the intercellular spaces and lamina propria were similar in size to individual droplets within conglomerates. We conclude that triacylglycerols are elaborated in the smooth endoplasmic reticulum, transferred to and processed in the Golgi region and finally discharged serosally as chylomicron-like particles of not greater than 400 nm diameter.", "contents": "An electron microscopic study of lipid absorption in the pyloric caeca of rainbow trout (Salmo gairdnerii) fed wax ester--rich zooplankton. Rainbow trout were killed 4 and 18 h after being fed wax ester-rich marine zooplankton and the absorptive epithelium of the pyloric caeca examined by electron microscopy. Numerous osmiophilic drops were seen in the lamina propria underlying the epithelium of fish killed at both times, but these drops were only abundant within columnar epithelial cells of fish killed 4 h after feeding. Pinocytotic profiles were not common at the luminal plasma membranes, nor were osmiophilic droplets seen in the terminal web area between the luminal plasma membrane and the extensive smooth endoplasmic reticulum. Numerous osmiophilic droplets, 30--100 nm in diameter, were present in the cisternae of the smooth endoplasmic reticulum with up to five separate droplets per individual cisterna. Columnar epithelial cells also contained up to 100 large osmiophilic drops (\"conglomerates\") which tended to be concentrated in the supranuclear (Golgi) regions. The conglomerates were 250--1200 nm in diameter and were themselves made up of smaller droplets 30--400 nm in diameter. Conglomerates were present both within intracellular membranes and free in the cytoplasm. Osmiophilic droplets in the intercellular spaces and lamina propria were similar in size to individual droplets within conglomerates. We conclude that triacylglycerols are elaborated in the smooth endoplasmic reticulum, transferred to and processed in the Golgi region and finally discharged serosally as chylomicron-like particles of not greater than 400 nm diameter."}
{"id": "PMID:487413", "title": "In vitro recalcification of the demineralized shell-repair membrane of the snail, Helix pomatia L.", "content": "The role of the amoebocytes in the calcification process of the shell-repair membrane of the snail, Helix pomatia, was investigated in vitro. The shell-repair membranes were demineralized with 0.5 M EDTA at pH 7.4. For the recalcification of the demineralized membranes two substrates were chosen: (i) Tris-buffered Helix pomatia-saline, pH 7.4, and (ii) Helix pomatia-saline supplemented with 5 mM CaCl2 and 5mM NaHCO3. The membranes were incubated in 2 ml substrate at 37 degrees C and examined after 2 h, 24 h, and 3, 5 and 7 days. Calcium deposition and crystal formation were observed within the membranes incubated in the salt-supplemented substrate. The control membranes were either heat-inactivated or deprived of lipids. No calcium precipitation was observed in control membranes. The experiments show that the recalcification of the shell-repair membrane is under strict cellular control and that the granules released from the amoebocytes serve as sites for calcium deposition. The role of phospholipids in the calcification process is discussed.", "contents": "In vitro recalcification of the demineralized shell-repair membrane of the snail, Helix pomatia L. The role of the amoebocytes in the calcification process of the shell-repair membrane of the snail, Helix pomatia, was investigated in vitro. The shell-repair membranes were demineralized with 0.5 M EDTA at pH 7.4. For the recalcification of the demineralized membranes two substrates were chosen: (i) Tris-buffered Helix pomatia-saline, pH 7.4, and (ii) Helix pomatia-saline supplemented with 5 mM CaCl2 and 5mM NaHCO3. The membranes were incubated in 2 ml substrate at 37 degrees C and examined after 2 h, 24 h, and 3, 5 and 7 days. Calcium deposition and crystal formation were observed within the membranes incubated in the salt-supplemented substrate. The control membranes were either heat-inactivated or deprived of lipids. No calcium precipitation was observed in control membranes. The experiments show that the recalcification of the shell-repair membrane is under strict cellular control and that the granules released from the amoebocytes serve as sites for calcium deposition. The role of phospholipids in the calcification process is discussed."}
{"id": "PMID:487416", "title": "The effect of supine rotation on left ventricular dimensions in man: a study using radio-opaque epicardial markers.", "content": "It has been reported that patient rotation into the left lateral decubitus position (30 degrees rao) produces significant changes in the regression equations used for left ventricular volume determination and that normal values for echocardiographic left ventricular dimensions obtained from supine patients differ from those obtained after rotation. The purpose of this study was to establish whether patient rotation is associated with changes in left ventricular size and systolic function. The distances between left ventricular epicardial markers attached at the time of cardiac surgery were measured using biplane cineradiography in 14 patients in order to determine left ventricular dimensions before and after rotation. Supine epicardial left ventricular dimensions were not significantly different from those obtained with the patient rotated 30 degrees rao. These results suggest that reported changes in invasive and noninvasive measurements of left ventricular function associated with patient rotation are not primarily due to changes in left ventricular size.", "contents": "The effect of supine rotation on left ventricular dimensions in man: a study using radio-opaque epicardial markers. It has been reported that patient rotation into the left lateral decubitus position (30 degrees rao) produces significant changes in the regression equations used for left ventricular volume determination and that normal values for echocardiographic left ventricular dimensions obtained from supine patients differ from those obtained after rotation. The purpose of this study was to establish whether patient rotation is associated with changes in left ventricular size and systolic function. The distances between left ventricular epicardial markers attached at the time of cardiac surgery were measured using biplane cineradiography in 14 patients in order to determine left ventricular dimensions before and after rotation. Supine epicardial left ventricular dimensions were not significantly different from those obtained with the patient rotated 30 degrees rao. These results suggest that reported changes in invasive and noninvasive measurements of left ventricular function associated with patient rotation are not primarily due to changes in left ventricular size."}
{"id": "PMID:487417", "title": "Intracardiac M-mode echocardiography for continuous left ventricular monitoring: method and potential application.", "content": "Because no accurate and readily applied method exists for continuous recording of left ventricular cavity dimensions and wall motion in man, we designed a catheter-mounted echocardiographic probe. The purpose of this report is to describe the instrumentation, technique, limitations, complications and preliminary observations employing the probe in humans. The echo transducer built into a cardiac allowed positioning in the right heart under fluoroscopy. The echo signals permitted continuous left ventricular cavity measurements at rest and during maneuvers. Our results suggest that intracardiac echo may be able to provide clear definition of motion patterns of the mitral valve and left ventricular walls. The ultrasonic catheter is an instrument that potentially offers a new technique for continuous monitoring of left ventricular size and motion.", "contents": "Intracardiac M-mode echocardiography for continuous left ventricular monitoring: method and potential application. Because no accurate and readily applied method exists for continuous recording of left ventricular cavity dimensions and wall motion in man, we designed a catheter-mounted echocardiographic probe. The purpose of this report is to describe the instrumentation, technique, limitations, complications and preliminary observations employing the probe in humans. The echo transducer built into a cardiac allowed positioning in the right heart under fluoroscopy. The echo signals permitted continuous left ventricular cavity measurements at rest and during maneuvers. Our results suggest that intracardiac echo may be able to provide clear definition of motion patterns of the mitral valve and left ventricular walls. The ultrasonic catheter is an instrument that potentially offers a new technique for continuous monitoring of left ventricular size and motion."}
{"id": "PMID:487418", "title": "Aortic vasa vasorum in cyanotic congenital heart disease.", "content": "Three cases for longstanding untreated cyanotic congenital heart disease are presented. In addition to collateral circulation from bronchial, intercostal, and inferior phrenic arteries, thoracic aortic vasa vasorum were visualized. We believe this may represent an additional collateral pathway to the lungs. The angiography of these cases is presented.", "contents": "Aortic vasa vasorum in cyanotic congenital heart disease. Three cases for longstanding untreated cyanotic congenital heart disease are presented. In addition to collateral circulation from bronchial, intercostal, and inferior phrenic arteries, thoracic aortic vasa vasorum were visualized. We believe this may represent an additional collateral pathway to the lungs. The angiography of these cases is presented."}
{"id": "PMID:487419", "title": "Massive hemoptysis secondary to flow-directed thermodilution catheters.", "content": "Hemoptysis is an unusual complication of flow-directed (Swan-Ganz) catheters. Over-inflation of the balloon with a shearing-induced rupture of a small pulmonary artery, and the spear effect of the catheter tip appear to be the mechanisms in the two cases presented. Diligent care to avoid overinflation of the balloon in the pulmonary capillary wedge position by observation of the pressure waveform is critical. The spear effect that is frequently seen during insertion may be eliminated by deflating the balloon at the first appearance of the pulmonary artery waveform and gradual advancement of the catheter five to eight cm, when the balloon is then reinflated to obtain the wedge.", "contents": "Massive hemoptysis secondary to flow-directed thermodilution catheters. Hemoptysis is an unusual complication of flow-directed (Swan-Ganz) catheters. Over-inflation of the balloon with a shearing-induced rupture of a small pulmonary artery, and the spear effect of the catheter tip appear to be the mechanisms in the two cases presented. Diligent care to avoid overinflation of the balloon in the pulmonary capillary wedge position by observation of the pressure waveform is critical. The spear effect that is frequently seen during insertion may be eliminated by deflating the balloon at the first appearance of the pulmonary artery waveform and gradual advancement of the catheter five to eight cm, when the balloon is then reinflated to obtain the wedge."}
{"id": "PMID:487420", "title": "Coronary artery-left ventricular communications: a report of two cases and review of the literature.", "content": "Two patients with chest pain had angiographically-demonstrated communications between the three coronary arteries and the left ventricular chamber. Communications between coronary arteries and the left ventricle are unusual and communications between all three coronary arteries and the left ventricle are rare. These anomalies are, however, commonly associated with symptoms of chest pain. The presence of left ventricular hypertrophy and a widened pulse pressure may suggest a greater hemodynamic effect of the shunt flow than often suspected angiographically.", "contents": "Coronary artery-left ventricular communications: a report of two cases and review of the literature. Two patients with chest pain had angiographically-demonstrated communications between the three coronary arteries and the left ventricular chamber. Communications between coronary arteries and the left ventricle are unusual and communications between all three coronary arteries and the left ventricle are rare. These anomalies are, however, commonly associated with symptoms of chest pain. The presence of left ventricular hypertrophy and a widened pulse pressure may suggest a greater hemodynamic effect of the shunt flow than often suspected angiographically."}
{"id": "PMID:487421", "title": "Percutaneous preformed single catheter coronary arteriography and its complications--10,000 cases.", "content": "The single preformed catheter has offered an alternative to the conventional approaches to study of the coronary circulation and ventriculography. It has the advantages of being introduced percutaneouly without multiple changes for routine study or for provocative drug testing such as for spasm with Ergonovine maleate or muscular bridges with nitroglycerine. It requires no arteriotomy and has been associated with a low complication rate.", "contents": "Percutaneous preformed single catheter coronary arteriography and its complications--10,000 cases. The single preformed catheter has offered an alternative to the conventional approaches to study of the coronary circulation and ventriculography. It has the advantages of being introduced percutaneouly without multiple changes for routine study or for provocative drug testing such as for spasm with Ergonovine maleate or muscular bridges with nitroglycerine. It requires no arteriotomy and has been associated with a low complication rate."}
{"id": "PMID:487424", "title": "Quantitative coronary arteriography using 105-MM photospot angiography and an optical magnifying device.", "content": "A quantitative coronary arteriographic technique is described utilizing 105-mm photospot angiography and a hand-held optical magnifying device. This technique is rapid, accurate, and reproducible. We feel this technique has potential in evaluating coronary responses to acute interventions and in the long term evaluation of progression or regression of atherosclerotic disease.", "contents": "Quantitative coronary arteriography using 105-MM photospot angiography and an optical magnifying device. A quantitative coronary arteriographic technique is described utilizing 105-mm photospot angiography and a hand-held optical magnifying device. This technique is rapid, accurate, and reproducible. We feel this technique has potential in evaluating coronary responses to acute interventions and in the long term evaluation of progression or regression of atherosclerotic disease."}
{"id": "PMID:487425", "title": "Inexpensive radiation protective glasses.", "content": "Using the services of a local optical supply house, fabrication and testing of eyeglasses of a high lead content that could be used to protect the eye from the hazards of radiation were evaluated. Two separate types of eyeglasses were manufactured (a prototype followed by a second pair), and these were tested against a commercially available pair of radiation protective glasses. The protection offered by the locally manufactured pair of glasses exceeded the commercial pair and the cost savings are significant.", "contents": "Inexpensive radiation protective glasses. Using the services of a local optical supply house, fabrication and testing of eyeglasses of a high lead content that could be used to protect the eye from the hazards of radiation were evaluated. Two separate types of eyeglasses were manufactured (a prototype followed by a second pair), and these were tested against a commercially available pair of radiation protective glasses. The protection offered by the locally manufactured pair of glasses exceeded the commercial pair and the cost savings are significant."}
{"id": "PMID:487426", "title": "Percutaneous cannulation of large pericardial effusions.", "content": "A rapid, easy, and safe method of introducing a large cannula into the pericardial space for drainage of large pericardial effusions using conventional catheter introducers and catheters is described. Confirmation of the presence of a sizeable pericardial effusion is essential before insertion of a percutaneous cannula.", "contents": "Percutaneous cannulation of large pericardial effusions. A rapid, easy, and safe method of introducing a large cannula into the pericardial space for drainage of large pericardial effusions using conventional catheter introducers and catheters is described. Confirmation of the presence of a sizeable pericardial effusion is essential before insertion of a percutaneous cannula."}
{"id": "PMID:487427", "title": "Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression.", "content": "The mechanism by which prolactin, a peptide hormone, regulates casein gene expression has been studied in mammary gland organ culture. After prolactin addition, a 2-4 fold increase in the rate of casein mRNA transcription was observed within 1 hr and maintained for at least 24 hr. This increased rate of transcription is not sufficient to account for the mass accumulation of casein mRNA. The half-life of casein mRNA is also increased 17-25 fold in the presence of prolactin. This change in casein mRNA half-life, coupled with a 2-4 fold increase in the rate of transcription, can account for the normal accumulation of casein mRNA observed after prolactin addition. This hormone-induced change in casein mRNA half-life appeared to be selective, since prolactin was found to exert only a slight effect (1-4 fold) on the half-life of poly(A) RNA determined under identical pulse-chase conditions. The hormonal regulation of casein gene expression thus does not app-ar to be an \"all or none\" process occurring only at the transcriptional or post-transcriptional levels, but rather may involve a coordinated response at several levels to permit the efficient expression of specialized differentiated functions.", "contents": "Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression. The mechanism by which prolactin, a peptide hormone, regulates casein gene expression has been studied in mammary gland organ culture. After prolactin addition, a 2-4 fold increase in the rate of casein mRNA transcription was observed within 1 hr and maintained for at least 24 hr. This increased rate of transcription is not sufficient to account for the mass accumulation of casein mRNA. The half-life of casein mRNA is also increased 17-25 fold in the presence of prolactin. This change in casein mRNA half-life, coupled with a 2-4 fold increase in the rate of transcription, can account for the normal accumulation of casein mRNA observed after prolactin addition. This hormone-induced change in casein mRNA half-life appeared to be selective, since prolactin was found to exert only a slight effect (1-4 fold) on the half-life of poly(A) RNA determined under identical pulse-chase conditions. The hormonal regulation of casein gene expression thus does not app-ar to be an \"all or none\" process occurring only at the transcriptional or post-transcriptional levels, but rather may involve a coordinated response at several levels to permit the efficient expression of specialized differentiated functions."}
{"id": "PMID:487428", "title": "Production of mRNA in Chinese hamster cells: relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences.", "content": "We constructed cloned DNA sequences complementary to unselected mRNAs [poly(A)+ cytoplasmic RNA] from Chinese hamster ovary cells and used them in RNA:DNA hybridization experiments. Each cloned DNA hybridized a single mRNA from 1.3-3.5 kb in length. The relative rates of labeling (transcription rates) of nuclear RNA complementary to each individual DNA segment varied approximately 10 fold. The relative cytoplasmic concentration of the same specific RNA sequences in the mRNA after an equilibrium labeling of the cells varied approximately 100 fold. In addition, we estimated the sizes of the nuclear RNA precursor molecules to these cytoplasmic mRNAs. Four main conclusions arise from these studies. First, the primary RNA transcripts, which range in size from 2.4-13.5 kb, are 2-6 times larger than the mRNAs; second, each cloned DNA segment is complementary to only one species of mRNA; third, for the RNA complementary to at least three of the nine cloned DNA segments, the relative cytoplasmic content is considerably different from the relative rate of nuclear RNA synthesis, suggesting the post-transcriptional events are involved in the determination of the cytoplasmic concentrations of some mammalian mRNAs; and fourth, the fraction of total nonribosomal nuclear RNA complementary to the nine cloned DNA segments is in most cases 10 fold less than the fraction of cytoplasmic mRNA complementary to the same cloned DNA segments, suggesting the synthesis of many hnRNA molecules that are qualitatively different from those which eventually contribute mRNA to the cytoplasm.", "contents": "Production of mRNA in Chinese hamster cells: relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences. We constructed cloned DNA sequences complementary to unselected mRNAs [poly(A)+ cytoplasmic RNA] from Chinese hamster ovary cells and used them in RNA:DNA hybridization experiments. Each cloned DNA hybridized a single mRNA from 1.3-3.5 kb in length. The relative rates of labeling (transcription rates) of nuclear RNA complementary to each individual DNA segment varied approximately 10 fold. The relative cytoplasmic concentration of the same specific RNA sequences in the mRNA after an equilibrium labeling of the cells varied approximately 100 fold. In addition, we estimated the sizes of the nuclear RNA precursor molecules to these cytoplasmic mRNAs. Four main conclusions arise from these studies. First, the primary RNA transcripts, which range in size from 2.4-13.5 kb, are 2-6 times larger than the mRNAs; second, each cloned DNA segment is complementary to only one species of mRNA; third, for the RNA complementary to at least three of the nine cloned DNA segments, the relative cytoplasmic content is considerably different from the relative rate of nuclear RNA synthesis, suggesting the post-transcriptional events are involved in the determination of the cytoplasmic concentrations of some mammalian mRNAs; and fourth, the fraction of total nonribosomal nuclear RNA complementary to the nine cloned DNA segments is in most cases 10 fold less than the fraction of cytoplasmic mRNA complementary to the same cloned DNA segments, suggesting the synthesis of many hnRNA molecules that are qualitatively different from those which eventually contribute mRNA to the cytoplasm."}
{"id": "PMID:487430", "title": "Isolation and transformation of primary mesenchymal cells of the chick embryo.", "content": "Pure primary mesenchymal cells from definitive streak stage chick embryos have been prepared free of epiblast and hypoblast cells. These cells have the potential in culture to differentiate into erythroid cells, beating heart muscle tissue, chondrocytes and epithelial cells. Transformation in vitro of pure primary mesenchymal cells by avian erythroblastosis virus (wt-AEV) and a temperature-sensitive mutant (ts34-AEV) gave rise to rapidly growing cells which remained largely undifferentiated, could be cloned in semi-solid medium and could be maintained for up to 3 months in culture. The majority of mesenchymal cells transformed by wt-AEV (MAE cells) are benzidine-negative. Gel electrophoresis of radioactively labeled cell proteins, immunoprecipitated with specific antisera against chicken hemoglobin, showed that MAE cell clones synthesize the alpha D, pi (or pi') and some unidentified \"globin\" polypeptide chains. Treatment of MAE cell clones with 1.0 mM n-butyrate stops cell proliferation reversibly and causes an increased synthesis of alpha D and pi (or pi') globin polypeptide chains. In certain clones of mesenchymal cells transformed by a temperature-sensitive mutant of the virus, ts34-AEV (MAE-ts34 cells), benzidine-positive cells can be induced by a shift from 37 degrees to 41 degrees C. The ability of the clone to undergo an increase in benzidine-positivity by temperature shift is decreased with the age of the clone. Different clones show a variable proportion of cells which are positive by immunofluorescence for both globin and chicken-specific histone H5. The alpha A and alpha D globin chains are synthesized in MAE-ts34 clones, but the ratios and quantities of these chains vary for different clones. Temperature shift made little difference in the types and quantities of globin chains synthesized; the increase in benzidine positivity is probably due to an increase in heme biosynthesis.", "contents": "Isolation and transformation of primary mesenchymal cells of the chick embryo. Pure primary mesenchymal cells from definitive streak stage chick embryos have been prepared free of epiblast and hypoblast cells. These cells have the potential in culture to differentiate into erythroid cells, beating heart muscle tissue, chondrocytes and epithelial cells. Transformation in vitro of pure primary mesenchymal cells by avian erythroblastosis virus (wt-AEV) and a temperature-sensitive mutant (ts34-AEV) gave rise to rapidly growing cells which remained largely undifferentiated, could be cloned in semi-solid medium and could be maintained for up to 3 months in culture. The majority of mesenchymal cells transformed by wt-AEV (MAE cells) are benzidine-negative. Gel electrophoresis of radioactively labeled cell proteins, immunoprecipitated with specific antisera against chicken hemoglobin, showed that MAE cell clones synthesize the alpha D, pi (or pi') and some unidentified \"globin\" polypeptide chains. Treatment of MAE cell clones with 1.0 mM n-butyrate stops cell proliferation reversibly and causes an increased synthesis of alpha D and pi (or pi') globin polypeptide chains. In certain clones of mesenchymal cells transformed by a temperature-sensitive mutant of the virus, ts34-AEV (MAE-ts34 cells), benzidine-positive cells can be induced by a shift from 37 degrees to 41 degrees C. The ability of the clone to undergo an increase in benzidine-positivity by temperature shift is decreased with the age of the clone. Different clones show a variable proportion of cells which are positive by immunofluorescence for both globin and chicken-specific histone H5. The alpha A and alpha D globin chains are synthesized in MAE-ts34 clones, but the ratios and quantities of these chains vary for different clones. Temperature shift made little difference in the types and quantities of globin chains synthesized; the increase in benzidine positivity is probably due to an increase in heme biosynthesis."}
{"id": "PMID:487431", "title": "Enhanced cellular fibronectin accumulation in chondrocytes treated with vitamin A.", "content": "Chick sternal chondrocytes cultured at high cell density lack fibronectin as a surface protein, while vitamin A-treated chondrocytes contain it as the major cell surface protein. We investigated the mechanism of fibronectin accumulation under these conditions. Control chondrocytes synthesized nearly as much fibronectin as vitamin A-treated chondrocytes, but it was secreted primarily into culture medium. Althought the fibronectin of control chondrocytes was of a slightly lower apparent molecular weight than the fibronectin synthesized by the treated cells, it bound as effectively to the cell layer of both normal and treated cells. In contrast, the vitamin A-treated cultures were 2.7 fold more effective in binding fibronectin synthesized by either control or treated cells. Thus in chondrocytes, vitamin A appears to regulate the cellular accumulation of fibronectin by increasing the ability of the cell layer to bind fibronectin rather than by altering its synthesis or its adhesivity for the cell layer.", "contents": "Enhanced cellular fibronectin accumulation in chondrocytes treated with vitamin A. Chick sternal chondrocytes cultured at high cell density lack fibronectin as a surface protein, while vitamin A-treated chondrocytes contain it as the major cell surface protein. We investigated the mechanism of fibronectin accumulation under these conditions. Control chondrocytes synthesized nearly as much fibronectin as vitamin A-treated chondrocytes, but it was secreted primarily into culture medium. Althought the fibronectin of control chondrocytes was of a slightly lower apparent molecular weight than the fibronectin synthesized by the treated cells, it bound as effectively to the cell layer of both normal and treated cells. In contrast, the vitamin A-treated cultures were 2.7 fold more effective in binding fibronectin synthesized by either control or treated cells. Thus in chondrocytes, vitamin A appears to regulate the cellular accumulation of fibronectin by increasing the ability of the cell layer to bind fibronectin rather than by altering its synthesis or its adhesivity for the cell layer."}
{"id": "PMID:487433", "title": "Programmed appearance of translatable flagellar tubulin mRNA during cell differentiation in Naegleria.", "content": "The programmed de novo synthesis of flagellar tubulin during the hour-long differentiation of Naegleria gruberi from amoebae to flagellates is our paradigm for the study of gene expression during cell differentiation. This paper reports the efficient translation of flagellar tubulin mRNA in the wheat germ cell-free system directed by total or polyadenylated RNA extracted from differentiating cells. The tubulin in the in vitro product has a subunit molecular weight of 55,000, separates into alpha and beta subunits under suitable conditions of polyacrylamide gel electrophoreis and co-polymerizes with calf brain tubulin. At least half of the tubulin synthesized in vitro is precipitated by antibodies specific to flagellar tubulin, and the immunoprecipitated tubulin subunits yield peptide maps similar to those of outer doublet tublin. Flagellar tubulin is the predominant protein synthesized in the cell-free system, and amounts to about 5% of the polypeptides whose synthesis is directed by total RNA from differentiating cells. In contrast, little or no flagellar tubulin is synthesized when the cell-free system is directed by RNA extracted from amoebae prior to differentiation. Translation assays show that at least 92% of the flagellar tubulin mRNA appears during differentiation. The time course of appearance of this mRNA was measured by quantitative immunoprecipitation of the cell-free products. Under conditions where cells from flagella 60 min after initiation of differentiation, translatable flagellar tubulin mRNA was first detected at 20 min, reached a maximum at about 60 min and then declined. An excellent correlation was observed between the amount of translatable flagellar tubulin mRNA and the previously measured rates of flagellar tubulin synthesis in vivo. These results indicate that synthesis of flagellar tubulin is a direct reflection of the abundance of its mRNA, and provide the molecular techniques for dissection of the factors that regulate the rapid appearance of this structural protein during differentiation.", "contents": "Programmed appearance of translatable flagellar tubulin mRNA during cell differentiation in Naegleria. The programmed de novo synthesis of flagellar tubulin during the hour-long differentiation of Naegleria gruberi from amoebae to flagellates is our paradigm for the study of gene expression during cell differentiation. This paper reports the efficient translation of flagellar tubulin mRNA in the wheat germ cell-free system directed by total or polyadenylated RNA extracted from differentiating cells. The tubulin in the in vitro product has a subunit molecular weight of 55,000, separates into alpha and beta subunits under suitable conditions of polyacrylamide gel electrophoreis and co-polymerizes with calf brain tubulin. At least half of the tubulin synthesized in vitro is precipitated by antibodies specific to flagellar tubulin, and the immunoprecipitated tubulin subunits yield peptide maps similar to those of outer doublet tublin. Flagellar tubulin is the predominant protein synthesized in the cell-free system, and amounts to about 5% of the polypeptides whose synthesis is directed by total RNA from differentiating cells. In contrast, little or no flagellar tubulin is synthesized when the cell-free system is directed by RNA extracted from amoebae prior to differentiation. Translation assays show that at least 92% of the flagellar tubulin mRNA appears during differentiation. The time course of appearance of this mRNA was measured by quantitative immunoprecipitation of the cell-free products. Under conditions where cells from flagella 60 min after initiation of differentiation, translatable flagellar tubulin mRNA was first detected at 20 min, reached a maximum at about 60 min and then declined. An excellent correlation was observed between the amount of translatable flagellar tubulin mRNA and the previously measured rates of flagellar tubulin synthesis in vivo. These results indicate that synthesis of flagellar tubulin is a direct reflection of the abundance of its mRNA, and provide the molecular techniques for dissection of the factors that regulate the rapid appearance of this structural protein during differentiation."}
{"id": "PMID:487434", "title": "Methylated and unmethylated DNA compartments in the sea urchin genome.", "content": "Sea urchin (Echinus esculentus) DNA has been separated into high and low molecular weight fractions by digestion with the mCpG-sensitive restriction endonucleases Hpa II, Hha I and Ava I. The separation was due to differences in methylation at the recognition sequences for these enzymes because an mCpG-insensitive isoschizomer of Hpa II (Msp I) digested Hpa II-resistant DNA to low molecular weight, showing that many Hpa II sites were in fact present in this fraction; and because 3H-methyl methionine administered to embryos was incorporated into the high molecular weight Hpa II-, Hha I- and Ava I-resistant fraction, but not significantly into the low molecular weight fraction. The fraction resistant to Hpa II, Hha I and Ava I amounted to about 40% of the total DNA. It consisted of long sequence tracts between 15 and well over 50 kg in length, in which many sites for each of these enzymes were methylated consecutively. The remaining 60% of the genome, (m-), was not significantly methylated. Methylated and unmethylated fractions were considered to be subfractions of the genome because enriched unique sequences from one fraction cross-reassociated poorly with the other fraction and specific sequences were found in either (m+) or (m-) but not in both (see below). Similar (m+) and (m-) compartments were found in embryos, germ cells and adult somatic tissues. Furthermor, we found no evidence for changes in the sequence composition of (m+) or (m-) between sperm, embryo or intestine DNAs, although low levels of exchange would not have been detected. Using cloned Echinus histone DNA, heterologous 5S DNA and ribosomal DNA probes, we have found that each of these gene families belongs to the unmethylated DNA compartment in all the tissues examined. In particular, there was no detectable methylation of histone DNA either in early embryos, which are thought to be actively transcribing the bulk of histone genes, or in sperm and gastrulae, in which most histone genes are not being transcribed. In contrast to these gene families, sequences complementary to an internally repetitious Echinus DNA clone were found primarily in the methylated DNA compartment.", "contents": "Methylated and unmethylated DNA compartments in the sea urchin genome. Sea urchin (Echinus esculentus) DNA has been separated into high and low molecular weight fractions by digestion with the mCpG-sensitive restriction endonucleases Hpa II, Hha I and Ava I. The separation was due to differences in methylation at the recognition sequences for these enzymes because an mCpG-insensitive isoschizomer of Hpa II (Msp I) digested Hpa II-resistant DNA to low molecular weight, showing that many Hpa II sites were in fact present in this fraction; and because 3H-methyl methionine administered to embryos was incorporated into the high molecular weight Hpa II-, Hha I- and Ava I-resistant fraction, but not significantly into the low molecular weight fraction. The fraction resistant to Hpa II, Hha I and Ava I amounted to about 40% of the total DNA. It consisted of long sequence tracts between 15 and well over 50 kg in length, in which many sites for each of these enzymes were methylated consecutively. The remaining 60% of the genome, (m-), was not significantly methylated. Methylated and unmethylated fractions were considered to be subfractions of the genome because enriched unique sequences from one fraction cross-reassociated poorly with the other fraction and specific sequences were found in either (m+) or (m-) but not in both (see below). Similar (m+) and (m-) compartments were found in embryos, germ cells and adult somatic tissues. Furthermor, we found no evidence for changes in the sequence composition of (m+) or (m-) between sperm, embryo or intestine DNAs, although low levels of exchange would not have been detected. Using cloned Echinus histone DNA, heterologous 5S DNA and ribosomal DNA probes, we have found that each of these gene families belongs to the unmethylated DNA compartment in all the tissues examined. In particular, there was no detectable methylation of histone DNA either in early embryos, which are thought to be actively transcribing the bulk of histone genes, or in sperm and gastrulae, in which most histone genes are not being transcribed. In contrast to these gene families, sequences complementary to an internally repetitious Echinus DNA clone were found primarily in the methylated DNA compartment."}
{"id": "PMID:487435", "title": "Characterization and transcription analysis of a cloned sequence derived from a major developmentally regulated mRNA of D. discoideum.", "content": "The plasmid pDd 812 contains a portion of a poly(A)+ RNA sequence isolated from developing cells of the cellular slime mold Dictyostelium discoideum (Williams and Lloyd, 1979). The poly(A)+ RNA complementary to this plasmid shows an increase in concentration during the first 4 hr of development followed by a decrease in concentration during the following 4 hr. This RNA is very abundant after 3-4 hr of development, constituting at least 2% of the poly(A)+ RNA population. In this study, we demonstrate that this poly(A)+ RNA is an mRNA sequence by translating the RNA complementary to pDd 812 in a heterologous system. The mRNA directs the synthesis of a major polypeptide of 33,000 daltons and a minor polypeptide of 31,000 daltons. We have used the plasmid DNA immobilized on filters to analyze the transcription of this RNA sequence in isolated nuclei. The amount of transcript synthesized in nuclei isolated at various stages of development which was complementary to pDd 812 changed in the same way as did the cytoplasmic concentration of this RNA--that is, maximal transcription occurred after 3-4 hr of development. Because this result was observed using labeling periods as short as 5 min, we believe that this change is unlikely to reflect a change in the rate of processing of RNA. We interpret these results to indicate that, at least in part, the control of the synthesis of this RNA is at the level of gene transcription.", "contents": "Characterization and transcription analysis of a cloned sequence derived from a major developmentally regulated mRNA of D. discoideum. The plasmid pDd 812 contains a portion of a poly(A)+ RNA sequence isolated from developing cells of the cellular slime mold Dictyostelium discoideum (Williams and Lloyd, 1979). The poly(A)+ RNA complementary to this plasmid shows an increase in concentration during the first 4 hr of development followed by a decrease in concentration during the following 4 hr. This RNA is very abundant after 3-4 hr of development, constituting at least 2% of the poly(A)+ RNA population. In this study, we demonstrate that this poly(A)+ RNA is an mRNA sequence by translating the RNA complementary to pDd 812 in a heterologous system. The mRNA directs the synthesis of a major polypeptide of 33,000 daltons and a minor polypeptide of 31,000 daltons. We have used the plasmid DNA immobilized on filters to analyze the transcription of this RNA sequence in isolated nuclei. The amount of transcript synthesized in nuclei isolated at various stages of development which was complementary to pDd 812 changed in the same way as did the cytoplasmic concentration of this RNA--that is, maximal transcription occurred after 3-4 hr of development. Because this result was observed using labeling periods as short as 5 min, we believe that this change is unlikely to reflect a change in the rate of processing of RNA. We interpret these results to indicate that, at least in part, the control of the synthesis of this RNA is at the level of gene transcription."}
{"id": "PMID:487438", "title": "Location of additional early gene sequences in the adenoviral chromosome.", "content": "Genetic and biochemical analysis has revealed additional early gene sequences in the group C human adenovirus genome. Physical mapping by an improved marker rescue method showed that a group of \"early function\" mutations previously found by us to map within the left quarter of the genome do not map within the known early sequence (0-11.2) in that region, but instead lie within the coordinates 18.5-22.0. A search for early expression in this region of the genome using saturation hybridization techniques showed the presence of two discrete sets of viral cytoplasmic RNA sequences, present early in infection at low copy number and complementary to sequences on the I strand of the adenovirus genome lying between 11.2-14.5 and 19.8-23.5 untis.", "contents": "Location of additional early gene sequences in the adenoviral chromosome. Genetic and biochemical analysis has revealed additional early gene sequences in the group C human adenovirus genome. Physical mapping by an improved marker rescue method showed that a group of \"early function\" mutations previously found by us to map within the left quarter of the genome do not map within the known early sequence (0-11.2) in that region, but instead lie within the coordinates 18.5-22.0. A search for early expression in this region of the genome using saturation hybridization techniques showed the presence of two discrete sets of viral cytoplasmic RNA sequences, present early in infection at low copy number and complementary to sequences on the I strand of the adenovirus genome lying between 11.2-14.5 and 19.8-23.5 untis."}
{"id": "PMID:487453", "title": "Conditional expression of the transformed phenotype in an \"epithelial-like\" cell line.", "content": "A subclone of ts 14/MNU/2, derived from a heteroploid (epithelial) monkey kidney cell line of BSC-1 and transformed in vitro by methylnitrosourea, expressed the transformed phenotype only at the elevated temperature of 39.5(0)C. Compared to the growth characteristics at 33(0)C, the transformed property was exhibited in the (1) high efficiency of plating at low cell densities, (2) colony morphology, (3) growth in absence of serum and (4) alterations in the quality and the quantity of chromosomal proteins in presence of low concentrations of serum. Such experimental systems may provide an effective control for studying molecular mechanisms related to transformation and neoplasia.", "contents": "Conditional expression of the transformed phenotype in an \"epithelial-like\" cell line. A subclone of ts 14/MNU/2, derived from a heteroploid (epithelial) monkey kidney cell line of BSC-1 and transformed in vitro by methylnitrosourea, expressed the transformed phenotype only at the elevated temperature of 39.5(0)C. Compared to the growth characteristics at 33(0)C, the transformed property was exhibited in the (1) high efficiency of plating at low cell densities, (2) colony morphology, (3) growth in absence of serum and (4) alterations in the quality and the quantity of chromosomal proteins in presence of low concentrations of serum. Such experimental systems may provide an effective control for studying molecular mechanisms related to transformation and neoplasia."}
{"id": "PMID:487454", "title": "Evaluation of a biochemical method for measuring platelet life-span.", "content": "Acetylsalicylic Acid (Aspirin) inhibits the formation of Malonaldehyde, a degradation product of the proaggregating Prostaglandins, during the life-span platelets in the circulating blood. After ingestion of 1.5 g of aspirin, there is a blockage of the formation of Malonaldehyde, followed by a progressive return to normal values, after an average of 8 days, in a healthy person. This fact is applied to the determination of half-life, found to be 3.7 +/- 1.3 days; a value in accord with those found by the isotopic method using 51Cr. The reproductibility of this method indicates a clinical application.", "contents": "Evaluation of a biochemical method for measuring platelet life-span. Acetylsalicylic Acid (Aspirin) inhibits the formation of Malonaldehyde, a degradation product of the proaggregating Prostaglandins, during the life-span platelets in the circulating blood. After ingestion of 1.5 g of aspirin, there is a blockage of the formation of Malonaldehyde, followed by a progressive return to normal values, after an average of 8 days, in a healthy person. This fact is applied to the determination of half-life, found to be 3.7 +/- 1.3 days; a value in accord with those found by the isotopic method using 51Cr. The reproductibility of this method indicates a clinical application."}
{"id": "PMID:487455", "title": "A three-dimensional study of organelle interrelationships in regenerating rat liver. 3. Organelles related to bile.", "content": "A number of cell structures are described which show a morphological relationship to the bile canaliculi. Two types of peribiliary vesicles are identified: osmication positive ones occurring between the bile canaliculi and the osmicated immature Golgi cisternae and probably deriving from the latter, and osmication negative ones related to MVB, on which they appear as buds. Small coated vesicles are seen attached to this second type. Large lacunae may originate from MVB, as suggested by the MVB-like internal vesicles they may contain. Some stay in luminal continuity with the bile canaliculi. Canalicular coated vesicles are seen as parts of the canalicular plasma membrane and free in the cytoplasm.", "contents": "A three-dimensional study of organelle interrelationships in regenerating rat liver. 3. Organelles related to bile. A number of cell structures are described which show a morphological relationship to the bile canaliculi. Two types of peribiliary vesicles are identified: osmication positive ones occurring between the bile canaliculi and the osmicated immature Golgi cisternae and probably deriving from the latter, and osmication negative ones related to MVB, on which they appear as buds. Small coated vesicles are seen attached to this second type. Large lacunae may originate from MVB, as suggested by the MVB-like internal vesicles they may contain. Some stay in luminal continuity with the bile canaliculi. Canalicular coated vesicles are seen as parts of the canalicular plasma membrane and free in the cytoplasm."}
{"id": "PMID:487456", "title": "A three-dimensional study of organelle interrelationships in regenerating rat liver. 4. Multivesicular bodies.", "content": "Double-membraned plasma-membrane-loops are formed from the plasma membrane and released into the cytoplasm. The vesiculation of mainly their inner membrane may transform them into MVB. The so-formed internal vesicles contain cytosol. In many MVB the surface of all their internal vesicles to gether corresponds well with the surface of their bordering membrane. This correlation may be the result of a dynamic equilibrium whereby degradation (and partial recyclage) of internal vesicles is compensated for by the formation of new internal vesicles. When this equilibrium becomes disturbed, MVB may show much less internal vesicles. Such 'depleted' MVB are often producing peribiliary vesicles and may themselves transform into lacunate emptying into the bile canaliculi.", "contents": "A three-dimensional study of organelle interrelationships in regenerating rat liver. 4. Multivesicular bodies. Double-membraned plasma-membrane-loops are formed from the plasma membrane and released into the cytoplasm. The vesiculation of mainly their inner membrane may transform them into MVB. The so-formed internal vesicles contain cytosol. In many MVB the surface of all their internal vesicles to gether corresponds well with the surface of their bordering membrane. This correlation may be the result of a dynamic equilibrium whereby degradation (and partial recyclage) of internal vesicles is compensated for by the formation of new internal vesicles. When this equilibrium becomes disturbed, MVB may show much less internal vesicles. Such 'depleted' MVB are often producing peribiliary vesicles and may themselves transform into lacunate emptying into the bile canaliculi."}
{"id": "PMID:487493", "title": "[Traumatic injuries of the epiphyseal plate in the lower extremity of the tibia].", "content": "In a series of 110 fractures of the ankle in children the authors have observed 27 cases of \"at risque\" lesions: Mac Farland types of fractures and crush injuries. Epiphysiodesis occurs in all cases of crush injuries and in 25% of fracture-separation of the tibial malleolus. In order to prevent this complication the only procedure is to obtain an anatomical reduction. Desepiphysiodesis is uncommunly possible (2 cases) and rarely effective. In case of varus deformity an osteotomy must be performed and repeated if the child is still young.", "contents": "[Traumatic injuries of the epiphyseal plate in the lower extremity of the tibia]. In a series of 110 fractures of the ankle in children the authors have observed 27 cases of \"at risque\" lesions: Mac Farland types of fractures and crush injuries. Epiphysiodesis occurs in all cases of crush injuries and in 25% of fracture-separation of the tibial malleolus. In order to prevent this complication the only procedure is to obtain an anatomical reduction. Desepiphysiodesis is uncommunly possible (2 cases) and rarely effective. In case of varus deformity an osteotomy must be performed and repeated if the child is still young."}
{"id": "PMID:487494", "title": "[Recurrent and multiple pheochromocytoma (author's transl)].", "content": "One case of multiple pheochromocytoma with several unusual features is reported: 1 -- the multiple localizations which came on at ten years interval. 2 -- Association with renal artery stenosis. 3 -- Association with Von Hippel Lindau's disease. The authors comment on this case and those from the literature.", "contents": "[Recurrent and multiple pheochromocytoma (author's transl)]. One case of multiple pheochromocytoma with several unusual features is reported: 1 -- the multiple localizations which came on at ten years interval. 2 -- Association with renal artery stenosis. 3 -- Association with Von Hippel Lindau's disease. The authors comment on this case and those from the literature."}
{"id": "PMID:487495", "title": "[Is conservative treatment justified in the case of advanced infantile tuberculosis of the hip? (author's transl)].", "content": "In the case of advanced children tuberculosis of the hip, the authors propose a more active treatment than Wilkinson's, which is less conservative. A local curetting is important, and if the acetabulum reveals a geodic aspect, this surgery may be done by pelvic approach. After an orthopedic traction, the surgical method is more efficient and permits better flexibility of the hip, only to be noticed under general anesthesia. After surgery, this traction must be prolonged for a several months and kinesitherapic mobilisation must be associated. The excellent result, both clinical and radiographic obtained by the authors seems to justify the use of the above mentioned method.", "contents": "[Is conservative treatment justified in the case of advanced infantile tuberculosis of the hip? (author's transl)]. In the case of advanced children tuberculosis of the hip, the authors propose a more active treatment than Wilkinson's, which is less conservative. A local curetting is important, and if the acetabulum reveals a geodic aspect, this surgery may be done by pelvic approach. After an orthopedic traction, the surgical method is more efficient and permits better flexibility of the hip, only to be noticed under general anesthesia. After surgery, this traction must be prolonged for a several months and kinesitherapic mobilisation must be associated. The excellent result, both clinical and radiographic obtained by the authors seems to justify the use of the above mentioned method."}
{"id": "PMID:487496", "title": "[Disinsertion of the subscapularis muscle in the treatment of obstetric paralysis of the upper limb (author's transl)].", "content": "Proximal disinsertion of the subscapularis is an effective operation in the correction of an abnormal position in internal rotation of the shoulder. Such a decision should be taken only if the head of the humerus is still well rounded. Restoration of movement by muscular transfer of active external rotation is performed in subsequent months only if the teres minor and infraspinatus muscles do not recover spontaneously.", "contents": "[Disinsertion of the subscapularis muscle in the treatment of obstetric paralysis of the upper limb (author's transl)]. Proximal disinsertion of the subscapularis is an effective operation in the correction of an abnormal position in internal rotation of the shoulder. Such a decision should be taken only if the head of the humerus is still well rounded. Restoration of movement by muscular transfer of active external rotation is performed in subsequent months only if the teres minor and infraspinatus muscles do not recover spontaneously."}
{"id": "PMID:487497", "title": "[Growth disturbance of the forearm in multiple exostosis and dyschondroplasia (author's transl)].", "content": "Multiple exostosis and Dyschondroplasia (Ollier's disease) are two Osteochondrodysplasia with abnormal cartilagenous growth which hinder growth of the long bones especially. Among other locations, these diseases do not affect the forearms symetrically. The ulnar bone is more often and more severely affected which results in a progressive dislocation of the forearm. The radial shaft bands while the hand incurves medially. The radial head can even dislocate completely. All the deformities of the forearm create a severe handicap. The authors report their surgical experience. Preventive management is the ideal solution and should be carried out before the onset of a complete dislocation (especially before the dislocation of the radial head). Correction should also take growth into consideration. Logically, the shortest bone (the ulnar) should be lengthened and straightened. Since it is not feasible to reasonably correct all deformities entirely by the ulnar lengthening, it is necessary to not only straighten but also to shorten the radial bone. Only regular surveillance of the growth abnormalities throughout childhood can allow a precise evaluation of the required correction necessary at the time of surgery which should be a definite one stage procedure.", "contents": "[Growth disturbance of the forearm in multiple exostosis and dyschondroplasia (author's transl)]. Multiple exostosis and Dyschondroplasia (Ollier's disease) are two Osteochondrodysplasia with abnormal cartilagenous growth which hinder growth of the long bones especially. Among other locations, these diseases do not affect the forearms symetrically. The ulnar bone is more often and more severely affected which results in a progressive dislocation of the forearm. The radial shaft bands while the hand incurves medially. The radial head can even dislocate completely. All the deformities of the forearm create a severe handicap. The authors report their surgical experience. Preventive management is the ideal solution and should be carried out before the onset of a complete dislocation (especially before the dislocation of the radial head). Correction should also take growth into consideration. Logically, the shortest bone (the ulnar) should be lengthened and straightened. Since it is not feasible to reasonably correct all deformities entirely by the ulnar lengthening, it is necessary to not only straighten but also to shorten the radial bone. Only regular surveillance of the growth abnormalities throughout childhood can allow a precise evaluation of the required correction necessary at the time of surgery which should be a definite one stage procedure."}
{"id": "PMID:487498", "title": "[The surgical treatment of pectus excavatum (author's transl)].", "content": "The Authors who have operated on 120 cases of pectus excavatum, describe their own method of surgical correction. It consists of: -- Sternal mobilisation by sternotomies; -- Limited chondreal resections; -- Internal fixation. The Authors emphasize the interest of a good internal fixation which must allow normal breathing during the period of cartilage's strengthening. The use of long flexibles thread pins affords a good stability and flexibility and assures at long term good cosmetic results.", "contents": "[The surgical treatment of pectus excavatum (author's transl)]. The Authors who have operated on 120 cases of pectus excavatum, describe their own method of surgical correction. It consists of: -- Sternal mobilisation by sternotomies; -- Limited chondreal resections; -- Internal fixation. The Authors emphasize the interest of a good internal fixation which must allow normal breathing during the period of cartilage's strengthening. The use of long flexibles thread pins affords a good stability and flexibility and assures at long term good cosmetic results."}
{"id": "PMID:487499", "title": "[Cricopharyngeal achalasia, exceptional cause of dysphagia in newborn (author's transl)].", "content": "Dysphagia of the newborn and the infant presents with many varied causes, neurological, malformative, turmoral or functional. Among the latter, cricopharyngeal achalasia is an exceptional entity open to discussion. Suspected as early as the first month, presenting with difficulty in swallowing with choking episodes and coughing up of food without any obvious etiology. Profile roetgengraphic studies will allow the diagnosis to be made. The characteristic sign is a round and regular posterior narrowing on the posterior wall of the esophagus (C4 - C5 level) with enlargement of the hypopharynx. This narrowing is a constant finding. This pathological entity, an absence of relaxation of the cricopharyngeus muscle during the third period of swallowing, well-known in the adult patient, has only exceptionally been described among newborn children. In the following case study, cure has only been obtained by an extra mucosal myotomy of the esophagus at the age of seven months. The discussion, considering the five cases reported in pediatric journals, covers the diagnostic elements, the physiopathology of the disease, the risks presented during evolution without treatment. Therapy must be precocious and surgical.", "contents": "[Cricopharyngeal achalasia, exceptional cause of dysphagia in newborn (author's transl)]. Dysphagia of the newborn and the infant presents with many varied causes, neurological, malformative, turmoral or functional. Among the latter, cricopharyngeal achalasia is an exceptional entity open to discussion. Suspected as early as the first month, presenting with difficulty in swallowing with choking episodes and coughing up of food without any obvious etiology. Profile roetgengraphic studies will allow the diagnosis to be made. The characteristic sign is a round and regular posterior narrowing on the posterior wall of the esophagus (C4 - C5 level) with enlargement of the hypopharynx. This narrowing is a constant finding. This pathological entity, an absence of relaxation of the cricopharyngeus muscle during the third period of swallowing, well-known in the adult patient, has only exceptionally been described among newborn children. In the following case study, cure has only been obtained by an extra mucosal myotomy of the esophagus at the age of seven months. The discussion, considering the five cases reported in pediatric journals, covers the diagnostic elements, the physiopathology of the disease, the risks presented during evolution without treatment. Therapy must be precocious and surgical."}
{"id": "PMID:487500", "title": "[Ovarian cysts and tumors in neonates, infants and children (author's transl)].", "content": "Twenty-six girls, aged from 0 to 15 years, were operated, upon ovarian cysts and tumors from 1952 to 1977. Before the age of one year, 11 non-neoplastic cysts and 2 benign tumors were observed. After the age of one, 1 non-neoplastic cyst and 12 tumors: 8 germ-cell tumors, 3 tumors of specialized gonadal stroma, and one epithelial tumor. Three germ-cell tumors were malignant (1 girl dead of disease, 2 alive at 2 and 1 1/2 years). All three gonadal stromal tumors are benign (with a follow-up of 3 months, 4 and 20 years). Epidemiology, pathology prognosis and management of these tumors are discussed according the recent literature.", "contents": "[Ovarian cysts and tumors in neonates, infants and children (author's transl)]. Twenty-six girls, aged from 0 to 15 years, were operated, upon ovarian cysts and tumors from 1952 to 1977. Before the age of one year, 11 non-neoplastic cysts and 2 benign tumors were observed. After the age of one, 1 non-neoplastic cyst and 12 tumors: 8 germ-cell tumors, 3 tumors of specialized gonadal stroma, and one epithelial tumor. Three germ-cell tumors were malignant (1 girl dead of disease, 2 alive at 2 and 1 1/2 years). All three gonadal stromal tumors are benign (with a follow-up of 3 months, 4 and 20 years). Epidemiology, pathology prognosis and management of these tumors are discussed according the recent literature."}
{"id": "PMID:487501", "title": "[Renal lesions in children with acute traumatic abdominal bleeding (author's transl)].", "content": "In 22 cases we observed the association of post traumatic intra and retro peritoneal bleeding the latter due to kidney rupture. In such cases the usual policy consists in treating the abdominal visceral lesion first and post pone the management of the kidney rupture. In 13 cases we decided to undertake simultaneous surgical treatment of the abdominal and kidney lesion. The preoperative diagnosis was established by combination of exploratory peritoneal dialysis and I V P. The surgical exploration was achieved by means of medial incision. In 6/13 cases it disclosed kidney lesions which necessitated immediate nephrectomy. Our experience shows that one stage surgical procedure is beneficial to the patients.", "contents": "[Renal lesions in children with acute traumatic abdominal bleeding (author's transl)]. In 22 cases we observed the association of post traumatic intra and retro peritoneal bleeding the latter due to kidney rupture. In such cases the usual policy consists in treating the abdominal visceral lesion first and post pone the management of the kidney rupture. In 13 cases we decided to undertake simultaneous surgical treatment of the abdominal and kidney lesion. The preoperative diagnosis was established by combination of exploratory peritoneal dialysis and I V P. The surgical exploration was achieved by means of medial incision. In 6/13 cases it disclosed kidney lesions which necessitated immediate nephrectomy. Our experience shows that one stage surgical procedure is beneficial to the patients."}
{"id": "PMID:487502", "title": "[Synovial chondromatosis in children (author's transl)].", "content": "A serie of four patients is reviewed. We conclude that synovitis chondromatosis is very rare in children; the diagnosis, accepting Jaffe definition is not easy. In this serie age and localisation on the hip modify consequently our indication of the treatment, and explain some recurrence.", "contents": "[Synovial chondromatosis in children (author's transl)]. A serie of four patients is reviewed. We conclude that synovitis chondromatosis is very rare in children; the diagnosis, accepting Jaffe definition is not easy. In this serie age and localisation on the hip modify consequently our indication of the treatment, and explain some recurrence."}
{"id": "PMID:487503", "title": "[The epiphyseal defect in children (author's transl)].", "content": "The authors produced four cases which all show at first almost the same radiographical espect: an epiphysal defect of the knee. The first case developed in an inflammatory context and proved to be an osteomyelitis. The second case showed a begnin tumour on X-Ray examination a chondroblastoma. Then an epiphysal tuberculosis was produced which at first led to a rather unsettled diagnosis. The last case was a rare observation of chondrome. The diagnostic problems are frequent and besides possible infections localisations, the diagnosis of chondrablastome seems to be the most likely. Surgical approach of such epiphysal lesions is justified not only to corroborate etiology but also in order to avoid an increase in the volume that could impair the epiphysal plate endanger the growth.", "contents": "[The epiphyseal defect in children (author's transl)]. The authors produced four cases which all show at first almost the same radiographical espect: an epiphysal defect of the knee. The first case developed in an inflammatory context and proved to be an osteomyelitis. The second case showed a begnin tumour on X-Ray examination a chondroblastoma. Then an epiphysal tuberculosis was produced which at first led to a rather unsettled diagnosis. The last case was a rare observation of chondrome. The diagnostic problems are frequent and besides possible infections localisations, the diagnosis of chondrablastome seems to be the most likely. Surgical approach of such epiphysal lesions is justified not only to corroborate etiology but also in order to avoid an increase in the volume that could impair the epiphysal plate endanger the growth."}
{"id": "PMID:487504", "title": "[Brachial plexus birth injuries. An experimental study (author's transl)].", "content": "The authors present an experimental study of obstetrical Brachial plexus Palsy. They study traction injury to 16 Brachial pleux. The first lesions were always disruption of the upper roots C5 and C6. After that, the Lower Plexus (C7 - C8 - T1) was disrupted. The two upper roots are usually interrupted and surgical graft seen to be possible. On the Lower Plexus, avulsions are found and intercostal nerves transfer must be performed.", "contents": "[Brachial plexus birth injuries. An experimental study (author's transl)]. The authors present an experimental study of obstetrical Brachial plexus Palsy. They study traction injury to 16 Brachial pleux. The first lesions were always disruption of the upper roots C5 and C6. After that, the Lower Plexus (C7 - C8 - T1) was disrupted. The two upper roots are usually interrupted and surgical graft seen to be possible. On the Lower Plexus, avulsions are found and intercostal nerves transfer must be performed."}
{"id": "PMID:487505", "title": "[Xanthogranulomatous pyelonephritis in the infant. A report on 4 cases (author's transl)].", "content": "Four cases of xanthogranulomatous pyelonephritis in children are reported. In three cases, the disease took a diffuse form and in the fourth it was of a localised form. Around these cases, the authors review 39 case histories published between 1963 and 1978 and which involve only children. An over-all assessment of the 43 cases thus gathered together allows the general picture of xanthogranulomatous pyelonepn 9 cases, it was of the localised type; and in 4 cases, the pyelonephritis only involved one or another pyelon in patients with re-duplication. The diffuse type is more common in boys and the localised type in girls. Twenty-nine cases were found in children of less than five years. In 23 cases, there was a proteus infection. The etio-pathogenesis of xanthogranulomatous pyelonephritis remains obscure. The main clinical and therapeutic problems are dealt with.", "contents": "[Xanthogranulomatous pyelonephritis in the infant. A report on 4 cases (author's transl)]. Four cases of xanthogranulomatous pyelonephritis in children are reported. In three cases, the disease took a diffuse form and in the fourth it was of a localised form. Around these cases, the authors review 39 case histories published between 1963 and 1978 and which involve only children. An over-all assessment of the 43 cases thus gathered together allows the general picture of xanthogranulomatous pyelonepn 9 cases, it was of the localised type; and in 4 cases, the pyelonephritis only involved one or another pyelon in patients with re-duplication. The diffuse type is more common in boys and the localised type in girls. Twenty-nine cases were found in children of less than five years. In 23 cases, there was a proteus infection. The etio-pathogenesis of xanthogranulomatous pyelonephritis remains obscure. The main clinical and therapeutic problems are dealt with."}
{"id": "PMID:487506", "title": "[Endoscopical measures of length of intramural ureter in children with primary vesico-ureteral reflux (author's transl)].", "content": "153 endoscopical measures of the length of the intra-mural ureter in 81 children over 2 years have enabled us to ascertain that this segment of vesical ureter is significantly shorter in most patients with vesico-ureteral reflux. By contrast with other previous studies, we have not been able to use this examination for establishing the prognosis of the reflux, perhaps by lack of sufficiently prolonged follow-up However these data have been helpful to us in two circumstances and were complementary of the determination of aspect and position of ureteral meatus: a - When the child is operated for au unilateral reflux, a controlateral short intra-mural ureter suggests the value of a bilateral reimplantation to avoid the appearance of a controlateral reflux. b - When the child has an urinary infection without demonstrated reflux, a short intra-mural ureter suggests the value of a repeated cystography.", "contents": "[Endoscopical measures of length of intramural ureter in children with primary vesico-ureteral reflux (author's transl)]. 153 endoscopical measures of the length of the intra-mural ureter in 81 children over 2 years have enabled us to ascertain that this segment of vesical ureter is significantly shorter in most patients with vesico-ureteral reflux. By contrast with other previous studies, we have not been able to use this examination for establishing the prognosis of the reflux, perhaps by lack of sufficiently prolonged follow-up However these data have been helpful to us in two circumstances and were complementary of the determination of aspect and position of ureteral meatus: a - When the child is operated for au unilateral reflux, a controlateral short intra-mural ureter suggests the value of a bilateral reimplantation to avoid the appearance of a controlateral reflux. b - When the child has an urinary infection without demonstrated reflux, a short intra-mural ureter suggests the value of a repeated cystography."}
{"id": "PMID:487507", "title": "[Constipation in the infant (author's transl)].", "content": "The authors studied 102 cases of constipation in children. 76 of them went through a protocol of investigations : clinical, psychological, radiological and manometric. The authors suggest that so-called functional constipation is more often organic than is commonly thought.", "contents": "[Constipation in the infant (author's transl)]. The authors studied 102 cases of constipation in children. 76 of them went through a protocol of investigations : clinical, psychological, radiological and manometric. The authors suggest that so-called functional constipation is more often organic than is commonly thought."}
{"id": "PMID:487508", "title": "[The elasticity co-efficient of the rectal wall (E.C.R.P.). A prognostic test for Hirschsprung's disease (author's transl)].", "content": "In a situation where all authors are agreed on the significant morbidity of a colostomy in Hirschsprung's disease, the new test described by the authors would appear to help, if not \"resolve\" the always difficult choice between a colostomy and simple nursing in the intermeditary treatment of Hirschsprung's disease of the newborn.", "contents": "[The elasticity co-efficient of the rectal wall (E.C.R.P.). A prognostic test for Hirschsprung's disease (author's transl)]. In a situation where all authors are agreed on the significant morbidity of a colostomy in Hirschsprung's disease, the new test described by the authors would appear to help, if not \"resolve\" the always difficult choice between a colostomy and simple nursing in the intermeditary treatment of Hirschsprung's disease of the newborn."}
{"id": "PMID:487509", "title": "[Wiedemann-Beckwith syndrome heredity pattern (author's transl)].", "content": "The authors report a sibship of four members (two male and two female)displaying the entire clinical picture of the Wiedemann-Beckwith syndrome. There was no consanguinity and no antecedents could be found in a three-generation search. On this occasion, a review is made of what is known about the Wiedemann-Beckwith syndrome concerning its hereditary pattern, which can currently be considered as autosomal dominant with variable expressivity and incomplete penetrance, instead of recessive as it was formerly accepted This heredity pattern must be known in order to provide an accurate genetic advise.", "contents": "[Wiedemann-Beckwith syndrome heredity pattern (author's transl)]. The authors report a sibship of four members (two male and two female)displaying the entire clinical picture of the Wiedemann-Beckwith syndrome. There was no consanguinity and no antecedents could be found in a three-generation search. On this occasion, a review is made of what is known about the Wiedemann-Beckwith syndrome concerning its hereditary pattern, which can currently be considered as autosomal dominant with variable expressivity and incomplete penetrance, instead of recessive as it was formerly accepted This heredity pattern must be known in order to provide an accurate genetic advise."}
{"id": "PMID:487510", "title": "[Apple peel small bowel. Report of two cases of a peculiar type of small bowel atresia (author's transl)].", "content": "The authors report two personal cases of a peculiar type of small bowel atresia called \"Apple peel small bowel\", they give proheminence on the unusualiness of the lesion, the non specific clinical picture, and the relatively bad prognosis. The physiopathologic hypothesis which were proposed for this disease are reviewed.", "contents": "[Apple peel small bowel. Report of two cases of a peculiar type of small bowel atresia (author's transl)]. The authors report two personal cases of a peculiar type of small bowel atresia called \"Apple peel small bowel\", they give proheminence on the unusualiness of the lesion, the non specific clinical picture, and the relatively bad prognosis. The physiopathologic hypothesis which were proposed for this disease are reviewed."}
{"id": "PMID:487511", "title": "[Segmental dilatation of the ileum associated with an omphalocele and a common mesentery. Discussion around 2 cases : chance or morphogenically \"related\"? (author's transl)].", "content": "Segmental dilatation of the ileum is the subject of this report which concerns two cases. Both cases were associated with omphalocele and intestinal malrotation, an observation already noticed by some authors. Is it still a hazardous coincidence or rather a real opportunity which throws light over the explanation of this malformation?", "contents": "[Segmental dilatation of the ileum associated with an omphalocele and a common mesentery. Discussion around 2 cases : chance or morphogenically \"related\"? (author's transl)]. Segmental dilatation of the ileum is the subject of this report which concerns two cases. Both cases were associated with omphalocele and intestinal malrotation, an observation already noticed by some authors. Is it still a hazardous coincidence or rather a real opportunity which throws light over the explanation of this malformation?"}
{"id": "PMID:487512", "title": "[Strangulation of the toes by hair (author's transl)].", "content": "The purpose of this report is to call attention to human hair as a potential source of injury to young children's toes. The toe tourniquest syndrom is an entity described by Quinn but the autors suspected intentional wrapping.", "contents": "[Strangulation of the toes by hair (author's transl)]. The purpose of this report is to call attention to human hair as a potential source of injury to young children's toes. The toe tourniquest syndrom is an entity described by Quinn but the autors suspected intentional wrapping."}
{"id": "PMID:487513", "title": "[Incontinence after repair of imperforate anus : treatment by a free muscle transplant (author's transl)].", "content": "A case of severe anal incontinence after multiple surgical repairs for ano-rectal imperforation is presented. A successful formation of a new puborectalis sling by means of free autogenous palmaris longus muscle transplantation according to Hakeliuis is reported. The clinical success is confirmed radiologically and electromyographically. The authors discussed the principles and the results of this new method. They believe that this case pleads in favour of true muscle autotransplant re-innervation.", "contents": "[Incontinence after repair of imperforate anus : treatment by a free muscle transplant (author's transl)]. A case of severe anal incontinence after multiple surgical repairs for ano-rectal imperforation is presented. A successful formation of a new puborectalis sling by means of free autogenous palmaris longus muscle transplantation according to Hakeliuis is reported. The clinical success is confirmed radiologically and electromyographically. The authors discussed the principles and the results of this new method. They believe that this case pleads in favour of true muscle autotransplant re-innervation."}
{"id": "PMID:487514", "title": "[The childs-philips intestinal plication. A long term follow-up of 11 patients (author's transl)].", "content": "The authors report a long term follow up of 11 children who underwent a Childs-Philips intestinal plication from one to fourteen years ago. One death was caused by this operation. Besides, the tolerance of the Childs-Philips operation seems to be good, the nine other patients present a normal growth without any recurrence of the occlusion. The authors emphasize the interest of this bowel plication in the treatment of occlusion during the childhood.", "contents": "[The childs-philips intestinal plication. A long term follow-up of 11 patients (author's transl)]. The authors report a long term follow up of 11 children who underwent a Childs-Philips intestinal plication from one to fourteen years ago. One death was caused by this operation. Besides, the tolerance of the Childs-Philips operation seems to be good, the nine other patients present a normal growth without any recurrence of the occlusion. The authors emphasize the interest of this bowel plication in the treatment of occlusion during the childhood."}
{"id": "PMID:487515", "title": "[The place of lavage-irrigation of the peritonium in peritonitis from the appendix in children. A report of 10 cases (author's transl)].", "content": "Confronted with the difficult problems presented in acute appendicular peritonitis, whether generalized or multiple focused, the authors used the \"lavage irrigation\" method in ten cases. They analysed the local and general conditions that led them to employ this method, in light of the evolution of this technic which they had observed in their experiences whit it. They have attempted to elucidate, using information previously available in the literature, the indications, the steps, and the eventual complications of the \"lavage irrigation\" of the peritonemum in the child.", "contents": "[The place of lavage-irrigation of the peritonium in peritonitis from the appendix in children. A report of 10 cases (author's transl)]. Confronted with the difficult problems presented in acute appendicular peritonitis, whether generalized or multiple focused, the authors used the \"lavage irrigation\" method in ten cases. They analysed the local and general conditions that led them to employ this method, in light of the evolution of this technic which they had observed in their experiences whit it. They have attempted to elucidate, using information previously available in the literature, the indications, the steps, and the eventual complications of the \"lavage irrigation\" of the peritonemum in the child."}
{"id": "PMID:487516", "title": "[End to side porto-caval shunt in familial type II homozygote hypercholesterolemia (author's transl)].", "content": "Two patients with homozygous type II familial hypercholemia have had significant improvement by end to side porta-caval shunt. Follow-up is respectively 1 and 4 years. When all the other treatment have failed in lowering blood cholesterol and L.D.L. levels, a portal diversion may help to prevent cardio-vascular atherosclerotic complications in the pediatric age.", "contents": "[End to side porto-caval shunt in familial type II homozygote hypercholesterolemia (author's transl)]. Two patients with homozygous type II familial hypercholemia have had significant improvement by end to side porta-caval shunt. Follow-up is respectively 1 and 4 years. When all the other treatment have failed in lowering blood cholesterol and L.D.L. levels, a portal diversion may help to prevent cardio-vascular atherosclerotic complications in the pediatric age."}
{"id": "PMID:487519", "title": "Time course of increased collateral arterial and venous endothelial cell turnover after renal artery stenosis in the rat.", "content": "We induced left renal artery stenosis in rats and studied collateral arterial formation by angiography, histology, and radioautography with tritiated thymidine. Endothelial cell turnover was estimated by radioautography with tritiated thymidine in the periureteric blood supply of 10 normal and 38 collateral-forming kidneys 1 to 100 days after stenosis. Periureteric arterial endothelial cell labeling showed a highly significant (P less than 0.005) increase, apparent within 1 day and gradually falling as the vessels grew, until a baseline was reached in 35 days. A smaller but statistically significant increase in the labeling index also was found in endothelial cells of the renal vein during the first week (P less than 0.01), and had a similar time course. A marked increase in epithelial cell labeling in the ureters draining the stenotic kidneys also was evident (P less than 0.005). Thus, collateral vessel development is characterized by active DNA synthesis in the cellular elements which is maximal during the first week. A humoral factor is implicated in the vascular response by the parallel proliferation of venous and uretic cellular elements that are unlikely to experience the biophysical forces, such as increased blood flow or tangential wall force, which might stimulate proliferation in ther arterial vessels.", "contents": "Time course of increased collateral arterial and venous endothelial cell turnover after renal artery stenosis in the rat. We induced left renal artery stenosis in rats and studied collateral arterial formation by angiography, histology, and radioautography with tritiated thymidine. Endothelial cell turnover was estimated by radioautography with tritiated thymidine in the periureteric blood supply of 10 normal and 38 collateral-forming kidneys 1 to 100 days after stenosis. Periureteric arterial endothelial cell labeling showed a highly significant (P less than 0.005) increase, apparent within 1 day and gradually falling as the vessels grew, until a baseline was reached in 35 days. A smaller but statistically significant increase in the labeling index also was found in endothelial cells of the renal vein during the first week (P less than 0.01), and had a similar time course. A marked increase in epithelial cell labeling in the ureters draining the stenotic kidneys also was evident (P less than 0.005). Thus, collateral vessel development is characterized by active DNA synthesis in the cellular elements which is maximal during the first week. A humoral factor is implicated in the vascular response by the parallel proliferation of venous and uretic cellular elements that are unlikely to experience the biophysical forces, such as increased blood flow or tangential wall force, which might stimulate proliferation in ther arterial vessels."}
{"id": "PMID:487517", "title": "Disturbed maternal bereavement following infant death.", "content": "This report describes mental health intervention with the mothers of children with behavioural disturbances following infant death. Disturbed maternal bereavement disrupted the mother's relationships with their own children and related to maladaptive handling of prior losses. Treatment directed toward the recovery of memories and effects related to these losses led to more adaptive parent-child relationships. The implications for preventive interventions with families are discussed.", "contents": "Disturbed maternal bereavement following infant death. This report describes mental health intervention with the mothers of children with behavioural disturbances following infant death. Disturbed maternal bereavement disrupted the mother's relationships with their own children and related to maladaptive handling of prior losses. Treatment directed toward the recovery of memories and effects related to these losses led to more adaptive parent-child relationships. The implications for preventive interventions with families are discussed."}
{"id": "PMID:487518", "title": "Congruence of childhood sex-role identity and behaviour disturbances.", "content": "Thirty-eight children who were aged between 4 and 12 1/2 years were referred for potential gender disturbances and received independent assessments of sex-role behaviour and identity. Ratings of sex-role behaviour were based on psychodynamic, behavioural and psychometric procedures, with the behavioural and psychodynamic ratings most highly correlated. Two ratings of sex-role identity disturbance, derived from psychodynamic and behavioural orientations, yielded a strong interrelated agreement. The mean ratings of sex-role identity and behaviour disturbance were moderately correlated and provided a high degree of internal consistency. An overall diagnosis was derived for each subject by combining the behaviour and identity mean ratings. The findings are discussed in terms of the limitations of present knowledge on normal and deviant sex-role developmental processes.", "contents": "Congruence of childhood sex-role identity and behaviour disturbances. Thirty-eight children who were aged between 4 and 12 1/2 years were referred for potential gender disturbances and received independent assessments of sex-role behaviour and identity. Ratings of sex-role behaviour were based on psychodynamic, behavioural and psychometric procedures, with the behavioural and psychodynamic ratings most highly correlated. Two ratings of sex-role identity disturbance, derived from psychodynamic and behavioural orientations, yielded a strong interrelated agreement. The mean ratings of sex-role identity and behaviour disturbance were moderately correlated and provided a high degree of internal consistency. An overall diagnosis was derived for each subject by combining the behaviour and identity mean ratings. The findings are discussed in terms of the limitations of present knowledge on normal and deviant sex-role developmental processes."}
{"id": "PMID:487524", "title": "Effects of neural stimuli on blood flow through vasa vasorum in dogs.", "content": "The purpose of this study was to determine whether neural stimuli alter blood flow through vasa vasorum in the thoracic aorta. We measured flow with microspheres in anesthetized dogs and observed responses to sympathetic stimulation and baroreceptor stimulation. During these interventions, changes in arterial pressure were prevented with propranolol or an arterial reservoir, to minimize indirect effects on vasa vasorum mediated through changes in aortic wall tension. Electrical stimulation of the stellate ganglion at 10 Hz reduced blood flow to vasa vasorum in the thoracic aorta from 11 +/- 1.6 (mean +/- SE to 6.8 +/- 1.1 ml/min per 100 g (p less than 0.05). Aortic diameter, measured with a sonomicrometer technique, did not change during sympathetic stimulation. Thus, the reduction in blood flow through vasa vasorum during sympathetic stimulation appears to be a direct effect, and not the result of constriction of the aorta and compression of vasa. To determine effects of physiological alterations in neurogenic vasoconstrictor activity, we examined responses to stimulation of carotid baroreceptors. When pressure in isolated, perfused carotid sinus baroreceptors was raised from 81 +/- 3 to 198 +/- 2 mm Hg, blood flow to vasa vasorum of the thoracic aorta increased from 3.7 +/- 0.6 to 10 +/- 2.2 ml/min per 100 g (P less than 0.05). We conclude that vasa vasorum are responsive to neural stimuli, since they constrict during sympathetic stimulation and dilate in response to baroreceptor stimulation.", "contents": "Effects of neural stimuli on blood flow through vasa vasorum in dogs. The purpose of this study was to determine whether neural stimuli alter blood flow through vasa vasorum in the thoracic aorta. We measured flow with microspheres in anesthetized dogs and observed responses to sympathetic stimulation and baroreceptor stimulation. During these interventions, changes in arterial pressure were prevented with propranolol or an arterial reservoir, to minimize indirect effects on vasa vasorum mediated through changes in aortic wall tension. Electrical stimulation of the stellate ganglion at 10 Hz reduced blood flow to vasa vasorum in the thoracic aorta from 11 +/- 1.6 (mean +/- SE to 6.8 +/- 1.1 ml/min per 100 g (p less than 0.05). Aortic diameter, measured with a sonomicrometer technique, did not change during sympathetic stimulation. Thus, the reduction in blood flow through vasa vasorum during sympathetic stimulation appears to be a direct effect, and not the result of constriction of the aorta and compression of vasa. To determine effects of physiological alterations in neurogenic vasoconstrictor activity, we examined responses to stimulation of carotid baroreceptors. When pressure in isolated, perfused carotid sinus baroreceptors was raised from 81 +/- 3 to 198 +/- 2 mm Hg, blood flow to vasa vasorum of the thoracic aorta increased from 3.7 +/- 0.6 to 10 +/- 2.2 ml/min per 100 g (P less than 0.05). We conclude that vasa vasorum are responsive to neural stimuli, since they constrict during sympathetic stimulation and dilate in response to baroreceptor stimulation."}
{"id": "PMID:487525", "title": "The plasma membrane of leading pacemaker cells in the rabbit sinus node. A qualitative and quantitative ultrastructural analysis.", "content": "We studied the fine structure of the plasma membrane of electrophysiologically identified leading pacemaker cells from the rabbit sinus node, using both ultrathin sections of fixed tissue and replicas of freeze-cleaved material. We found that differences exist between sinus node and working myocardial membranes, but these are only quantitative. The caveolae or sarcolemmal invaginations are present in very large numbers; they increase the surface area of the plasma membrane by about 100%. The small macular nexuses that are present represent 0.2% of the membrane surface area. Nexuses are therefore about 10 times less numerous in leading sinus node cells than in working myocardium cells. A single equivalent electrical representation of the sinus node shows, nevertheless, that an appreciable electrical coupling may be expected.", "contents": "The plasma membrane of leading pacemaker cells in the rabbit sinus node. A qualitative and quantitative ultrastructural analysis. We studied the fine structure of the plasma membrane of electrophysiologically identified leading pacemaker cells from the rabbit sinus node, using both ultrathin sections of fixed tissue and replicas of freeze-cleaved material. We found that differences exist between sinus node and working myocardial membranes, but these are only quantitative. The caveolae or sarcolemmal invaginations are present in very large numbers; they increase the surface area of the plasma membrane by about 100%. The small macular nexuses that are present represent 0.2% of the membrane surface area. Nexuses are therefore about 10 times less numerous in leading sinus node cells than in working myocardium cells. A single equivalent electrical representation of the sinus node shows, nevertheless, that an appreciable electrical coupling may be expected."}
{"id": "PMID:487527", "title": "Modulating effect of regional myocardial performance on local myocardial perfusion in the dog.", "content": "We studied the effect of regional contractile performance on regional coronary blood flow and flow distribution in 10 dogs. The left anterior descending (LAD) coronary artery was cannulated and perfused. Maximal vasodilation was obtained with adenosine. Consequently, variations of LAD flow reflected changes of extravascular resistance. Lidocaine injected in the LAD caused a localized reduction of contractile performance as shown by the absence of systolic wall thickening. Global left ventricular performance and pressure were unchanged. Coronary extravascular resistance diminished and LAD flow increased from 4.8 +/- 0.5 to 6.2 +/- 0.6 ml/min per g (P less than 0.02). The endocardial: epicardial ratio increased from 1.02 +/- 0.07 to 1.28 +/- 0.07 (P less than 0.001). Isoproterenol in the LAD augmented systolic wall thickening. Regional coronary flow diminished from 5.1 +/- 0.5 to 3.3 +/- 0.4 ml/min per g (P less than 0.001), and the endocardial:epicardial ratio diminished from 1.08 +/- 0.07 to 0.75 +/- 0.07 (P less than 0.01). These data indicate that myocardial contractility is a major component of extravascular coronary resistance and is a mechanical determinant of coronary blood flow and its transmural distribution.", "contents": "Modulating effect of regional myocardial performance on local myocardial perfusion in the dog. We studied the effect of regional contractile performance on regional coronary blood flow and flow distribution in 10 dogs. The left anterior descending (LAD) coronary artery was cannulated and perfused. Maximal vasodilation was obtained with adenosine. Consequently, variations of LAD flow reflected changes of extravascular resistance. Lidocaine injected in the LAD caused a localized reduction of contractile performance as shown by the absence of systolic wall thickening. Global left ventricular performance and pressure were unchanged. Coronary extravascular resistance diminished and LAD flow increased from 4.8 +/- 0.5 to 6.2 +/- 0.6 ml/min per g (P less than 0.02). The endocardial: epicardial ratio increased from 1.02 +/- 0.07 to 1.28 +/- 0.07 (P less than 0.001). Isoproterenol in the LAD augmented systolic wall thickening. Regional coronary flow diminished from 5.1 +/- 0.5 to 3.3 +/- 0.4 ml/min per g (P less than 0.001), and the endocardial:epicardial ratio diminished from 1.08 +/- 0.07 to 0.75 +/- 0.07 (P less than 0.01). These data indicate that myocardial contractility is a major component of extravascular coronary resistance and is a mechanical determinant of coronary blood flow and its transmural distribution."}
{"id": "PMID:487529", "title": "Cellular thin filament protein contents and force generation in porcine arteries and veins.", "content": "We estimated the cellular myosin, actin, and tropomyosin contents of vascular smooth muscle from (1) seven major arteries, (2) seven large veins, and (3) the first through third order branches of the uterine vasculature to determine whether variations in the contractile apparatus contribute to the functional diversity of vascular smooth muscle. We obtained the estimates by quantitative densitometry of stained polyacrylamide gels after electrophoresis of sodium dodecyl sulfate-treated tissue homogenates. No differences in cellular myosin content were found (18.7 +/- 1.0 mg/g cell wet weight in arteries vs. 17.2 +/- 0.7 in veins). However, the actin and tropomyosin contents were higher in arteries (49.7 +/- 2.9 and 13.1 +/- 0.8 mg/g cell, respectively) than in veins (25.5 +/- 1.4 and 7.0 +/- 0.3 mg/g cell). These differences persisted in the smaller uterine vessels. The higher contents of thin filament proteins in arteries, compared with veins and several other smooth muscle tissues previously studied, may underlie the high force generating capacity of arterial smooth muscle.", "contents": "Cellular thin filament protein contents and force generation in porcine arteries and veins. We estimated the cellular myosin, actin, and tropomyosin contents of vascular smooth muscle from (1) seven major arteries, (2) seven large veins, and (3) the first through third order branches of the uterine vasculature to determine whether variations in the contractile apparatus contribute to the functional diversity of vascular smooth muscle. We obtained the estimates by quantitative densitometry of stained polyacrylamide gels after electrophoresis of sodium dodecyl sulfate-treated tissue homogenates. No differences in cellular myosin content were found (18.7 +/- 1.0 mg/g cell wet weight in arteries vs. 17.2 +/- 0.7 in veins). However, the actin and tropomyosin contents were higher in arteries (49.7 +/- 2.9 and 13.1 +/- 0.8 mg/g cell, respectively) than in veins (25.5 +/- 1.4 and 7.0 +/- 0.3 mg/g cell). These differences persisted in the smaller uterine vessels. The higher contents of thin filament proteins in arteries, compared with veins and several other smooth muscle tissues previously studied, may underlie the high force generating capacity of arterial smooth muscle."}
{"id": "PMID:487530", "title": "Dynamic changes in the canine mitral regurgitant orifice area during ventricular ejection.", "content": "We designed this study to test the hypothesis that in acute mitral regurgitation the mitral regurgitant area (MRA) is a dynamic quantity which varies with the time variation of ventricular volume. Mitral insufficiency was created in five open-chest dogs in which a portion of the anterior leaflet was excised. Phasic aortic and mitral flows were measured electromagnetically, along with left atrial and ventricular pressures. Filling, regurgitant, and stroke volumes, and systolic pressure gradient were determined by digital methods. MRA was calculated from the fluid dynamic equation of motion to give the temporal mean and the instantaneous value at three instants of time and at the time of peak flow (when inertia is negligible). Mean regurgitant fraction was 42 +/- 12% with no indication of left ventricular failure due to volume overload. MRA decreased monotonically with time to 59% of its initial value and closely paralleled the decrease in ventricular volume during systole. In a control study using a tilting-disc prosthesis with a hole 5 mm in diameter in the occluder, the calculated MRA was time invariant and equal to the measured area for regurgitation. We conclude that in acute mitral regurgitation the MRA is a function of ventricular volume.", "contents": "Dynamic changes in the canine mitral regurgitant orifice area during ventricular ejection. We designed this study to test the hypothesis that in acute mitral regurgitation the mitral regurgitant area (MRA) is a dynamic quantity which varies with the time variation of ventricular volume. Mitral insufficiency was created in five open-chest dogs in which a portion of the anterior leaflet was excised. Phasic aortic and mitral flows were measured electromagnetically, along with left atrial and ventricular pressures. Filling, regurgitant, and stroke volumes, and systolic pressure gradient were determined by digital methods. MRA was calculated from the fluid dynamic equation of motion to give the temporal mean and the instantaneous value at three instants of time and at the time of peak flow (when inertia is negligible). Mean regurgitant fraction was 42 +/- 12% with no indication of left ventricular failure due to volume overload. MRA decreased monotonically with time to 59% of its initial value and closely paralleled the decrease in ventricular volume during systole. In a control study using a tilting-disc prosthesis with a hole 5 mm in diameter in the occluder, the calculated MRA was time invariant and equal to the measured area for regurgitation. We conclude that in acute mitral regurgitation the MRA is a function of ventricular volume."}
{"id": "PMID:487531", "title": "Mechanism of action of methyldopa in the rat. Role of 3-O-methylated metabolites.", "content": "We studied the effect of 3-O-methyl-methyldopa (OMMD), the 3-O-methylated metabolite of the antihypertensive drug methyldopa (alpha-methyldopa, AMD), on blood pressure in the spontaneously hypertensive rat. OMMD lowered blood pressure in a dose-related manner when given orally or intraperitoneally. Its action lasted longer than that of AMD, and daily oral administration produced a cumulative fall in blood pressure. Oral and intraperitoneal OMMD produced similar reductions of blood pressure and similar tissue OMMD levels. After intraperitoneal injection of different doses, levels of OMMD measured in brain, spinal cord, and plasma correlated with the magnitude of the antihypertensive effect. No AMD was detected in tissues after either route of administration, which suggests that the antihypertensive effect was not based on demethylation of OMMD to AMD. Peripheral inhibition of the enzyme, aromatic amino acid decarboxylase (AAAD), failed to suppress OMMD's effect on blood pressure; in contrast, central inhibition of AAAD did decrease OMMD's antihypertensive effect. These observations suggest that 3-O-methylated metabolites may participate in the antihypertensive effect of AMD.", "contents": "Mechanism of action of methyldopa in the rat. Role of 3-O-methylated metabolites. We studied the effect of 3-O-methyl-methyldopa (OMMD), the 3-O-methylated metabolite of the antihypertensive drug methyldopa (alpha-methyldopa, AMD), on blood pressure in the spontaneously hypertensive rat. OMMD lowered blood pressure in a dose-related manner when given orally or intraperitoneally. Its action lasted longer than that of AMD, and daily oral administration produced a cumulative fall in blood pressure. Oral and intraperitoneal OMMD produced similar reductions of blood pressure and similar tissue OMMD levels. After intraperitoneal injection of different doses, levels of OMMD measured in brain, spinal cord, and plasma correlated with the magnitude of the antihypertensive effect. No AMD was detected in tissues after either route of administration, which suggests that the antihypertensive effect was not based on demethylation of OMMD to AMD. Peripheral inhibition of the enzyme, aromatic amino acid decarboxylase (AAAD), failed to suppress OMMD's effect on blood pressure; in contrast, central inhibition of AAAD did decrease OMMD's antihypertensive effect. These observations suggest that 3-O-methylated metabolites may participate in the antihypertensive effect of AMD."}
{"id": "PMID:487534", "title": "Exercise-induced U-wave inversion as a marker of stenosis of the left anterior descending coronary artery.", "content": "The prevalence and cineangiographic correlates of exercise-induced inversion of U waves were studied in 248 patients. Exercise-induced U-wave inversion was observed in 36 patients (15%), of whom 35 had greater than or equal to 75% stenosis in one or more of the major coronary arteries. The proximal left anterior descending or left main coronary artery was involved in 33 of these patients, including 24 patients with no electrocardiographic evidence of anterior myocardial infarction. Exercise-induced U-wave inversion was observed in the absence of an abnormal resting 12-lead ECG. Only one of the 82 patients (1.2%) without significant coronary artery disease demonstrated exercise-induced U-wave inversion, and this patient had a primary cardiomyopathy. We conclude that exercise-induced inversion of the U-wave is highly predictiveof significant coronary artery disease and, more specifically, of disease of the proximal left anterior descending coronary artery.", "contents": "Exercise-induced U-wave inversion as a marker of stenosis of the left anterior descending coronary artery. The prevalence and cineangiographic correlates of exercise-induced inversion of U waves were studied in 248 patients. Exercise-induced U-wave inversion was observed in 36 patients (15%), of whom 35 had greater than or equal to 75% stenosis in one or more of the major coronary arteries. The proximal left anterior descending or left main coronary artery was involved in 33 of these patients, including 24 patients with no electrocardiographic evidence of anterior myocardial infarction. Exercise-induced U-wave inversion was observed in the absence of an abnormal resting 12-lead ECG. Only one of the 82 patients (1.2%) without significant coronary artery disease demonstrated exercise-induced U-wave inversion, and this patient had a primary cardiomyopathy. We conclude that exercise-induced inversion of the U-wave is highly predictiveof significant coronary artery disease and, more specifically, of disease of the proximal left anterior descending coronary artery."}
{"id": "PMID:487536", "title": "Normal electrocardiographic waveform characteristics during treadmill exercise testing.", "content": "Forty asymptomatic male patients at low risk for cardiovascular disease completed maximal treadmill testing. Electrocardiograms from leads CC5, CM5, V5, Yh and Z were recorded across multiple pretest, exercise and recovery conditions. ECG waveforms were subsequently digitized, averaged and processed to provide Q-, R-, S- and T-wave amplitudes, ST-segment means and slopes, and QS- and RT-interval durations. Average R-wave amplitude increased during early exercise and then dramatically decreased to maximum effort. Average S-wave amplitude became greater as exercise progressed. Average J junction was slightly positive before exercise, became negative during exercise (except lead Z) and returned to zero after exercise. The ST-segment slope increased dramatically with progressive exercise. The response of T-wave amplitude, RT and QS intervals are also described. Separately, 22 asymptomatic male subjects each completed two maximal treadmill tests 2 weeks apart. ECG data acquisition and processing were similar to those noted above. Pooled, within-subject estimates of variability were computed for the ECG leads, ECG measurements and protocol conditions. These variability estimates are useful for interpreting ECG responses to exercise testing.", "contents": "Normal electrocardiographic waveform characteristics during treadmill exercise testing. Forty asymptomatic male patients at low risk for cardiovascular disease completed maximal treadmill testing. Electrocardiograms from leads CC5, CM5, V5, Yh and Z were recorded across multiple pretest, exercise and recovery conditions. ECG waveforms were subsequently digitized, averaged and processed to provide Q-, R-, S- and T-wave amplitudes, ST-segment means and slopes, and QS- and RT-interval durations. Average R-wave amplitude increased during early exercise and then dramatically decreased to maximum effort. Average S-wave amplitude became greater as exercise progressed. Average J junction was slightly positive before exercise, became negative during exercise (except lead Z) and returned to zero after exercise. The ST-segment slope increased dramatically with progressive exercise. The response of T-wave amplitude, RT and QS intervals are also described. Separately, 22 asymptomatic male subjects each completed two maximal treadmill tests 2 weeks apart. ECG data acquisition and processing were similar to those noted above. Pooled, within-subject estimates of variability were computed for the ECG leads, ECG measurements and protocol conditions. These variability estimates are useful for interpreting ECG responses to exercise testing."}
{"id": "PMID:487538", "title": "The electrocardiographic and antiarrhythmic effects of imipramine hydrochloride at therapeutic plasma concentrations.", "content": "The electrocardiographic effects of imipramine hydrochloride at therapeutic plasma concentrations were determined in 44 depressed patients during a 6-week clinical outcome study of depression. During each week of the protocol, i.e., 2 weeks of control and 4 weeks of drug treatment, a standard 12-lead ECG, high-speed, high-fidelity ECG tracings, and a 24-hour continuous ECG recording were obtained. PR, QRS, and QTc intervals, T-wave amplitude, heart rate and frequency of ventricular premature depolarizations (VPDs) were measured. The plasma concentration of imipramine and desmethylimipramine was measured three times a week. Imipramine prolonged the PR (p less than 0.001), QRS (p less than 0.001) and QTc (p less than 0.001) intervals, increased the heart rate (p less than 0.001) and lowered T-wave amplitude (p less than 0.05) during the 4 weeks of treatment. No patient developed high-grade atrioventricular block or severe intraventricular conduction abnormalities. In addition, imipramine had a potent antiarrhythmic action in patients who were recovering from depression. Ten of 11 patients who had more than 10 VPDs/hour had 90% or greater arrhythmia suppression during antidepressant treatment with imipramine at plasma concentrations ranging from 100--302 ng/ml.", "contents": "The electrocardiographic and antiarrhythmic effects of imipramine hydrochloride at therapeutic plasma concentrations. The electrocardiographic effects of imipramine hydrochloride at therapeutic plasma concentrations were determined in 44 depressed patients during a 6-week clinical outcome study of depression. During each week of the protocol, i.e., 2 weeks of control and 4 weeks of drug treatment, a standard 12-lead ECG, high-speed, high-fidelity ECG tracings, and a 24-hour continuous ECG recording were obtained. PR, QRS, and QTc intervals, T-wave amplitude, heart rate and frequency of ventricular premature depolarizations (VPDs) were measured. The plasma concentration of imipramine and desmethylimipramine was measured three times a week. Imipramine prolonged the PR (p less than 0.001), QRS (p less than 0.001) and QTc (p less than 0.001) intervals, increased the heart rate (p less than 0.001) and lowered T-wave amplitude (p less than 0.05) during the 4 weeks of treatment. No patient developed high-grade atrioventricular block or severe intraventricular conduction abnormalities. In addition, imipramine had a potent antiarrhythmic action in patients who were recovering from depression. Ten of 11 patients who had more than 10 VPDs/hour had 90% or greater arrhythmia suppression during antidepressant treatment with imipramine at plasma concentrations ranging from 100--302 ng/ml."}
{"id": "PMID:487539", "title": "Adverse hemodynamic effects of intravenous disopyramide compared with quinidine in conscious dogs.", "content": "Disopyramide resembles quinidine electrophysiologically, but its effect on left ventricular function has not been clarified. Twelve awake dogs were instrumented for measurement of cardiac output, left ventricular pressure and its maximal first derivative (dP/dt max), and left atrial and aortic pressures. Disopyramide or quinidine at identical, clinically relevant doses (1 and 5 mg/kg i.v.) was infused over 5 minutes at each level. Peak changes after disopyramide 1 mg/kg included increases in heart rate (34%), mean aortic pressure (24%) and systemic vascular resistance (33%), and decreases in stroke volume (16%) and dP/dt max (19%). With disopyramide at 5 mg/kg these changes were of greater magnitude (e.g., dP/dt -- 36%). Quinidine at both doses caused no changes except a 13% decrease in vascular resistance at 5 mg/kg. Heart rate with disopyramide increased after propranolol (1 mg/kg i.v.), was unchanged after atropine (0.1 mg/kg i.v.), and slowed after propranolol and atropine. Phenoxybenzamine (2 mg/kg i.v.) did not prevent the rise in systemic vascular resistance produced by disopyramide. Thus, disopyramide in clinical dosages exerts opposing direct and indirect effects on cardiac pacemakers and, unlike quinidine, is a potent myocardial depressant and vasoconstrictor in the conscious dog.", "contents": "Adverse hemodynamic effects of intravenous disopyramide compared with quinidine in conscious dogs. Disopyramide resembles quinidine electrophysiologically, but its effect on left ventricular function has not been clarified. Twelve awake dogs were instrumented for measurement of cardiac output, left ventricular pressure and its maximal first derivative (dP/dt max), and left atrial and aortic pressures. Disopyramide or quinidine at identical, clinically relevant doses (1 and 5 mg/kg i.v.) was infused over 5 minutes at each level. Peak changes after disopyramide 1 mg/kg included increases in heart rate (34%), mean aortic pressure (24%) and systemic vascular resistance (33%), and decreases in stroke volume (16%) and dP/dt max (19%). With disopyramide at 5 mg/kg these changes were of greater magnitude (e.g., dP/dt -- 36%). Quinidine at both doses caused no changes except a 13% decrease in vascular resistance at 5 mg/kg. Heart rate with disopyramide increased after propranolol (1 mg/kg i.v.), was unchanged after atropine (0.1 mg/kg i.v.), and slowed after propranolol and atropine. Phenoxybenzamine (2 mg/kg i.v.) did not prevent the rise in systemic vascular resistance produced by disopyramide. Thus, disopyramide in clinical dosages exerts opposing direct and indirect effects on cardiac pacemakers and, unlike quinidine, is a potent myocardial depressant and vasoconstrictor in the conscious dog."}
{"id": "PMID:487540", "title": "Histology of pulmonary arterial supply in pulmonary atresia with ventricular septal defect.", "content": "A histologic study was performed on 22 specimens of pulmonary atresia with ventricular septal defect to 1) ascertain the existence of the main pulmonary artery; 2) distinguish the ductus arteriosus from the systemic collateral arteries (SCA); 3) establish the nutritive or functional nature of collateral circulation; and 4) evaluate the morphology of the distal pulmonary bed. Three cases had absent main pulmonary artery, one with and two without signs of infundibular septation. We suggest that absent main pulmonary artery may exist with both infundibular pulmonary atresia and persistent truncus arteriosus. SCAs have been found to have similar histological features as systemic muscular arteries of the same size--their medial muscular layer merges gradually into an elastic one at different depth inside the lungs. Injection of contrast material allowed us to demonstrate that these vessels are functional, since they inosculate into efficient pulmonary arteries ending in the respiratory units. When the distal pulmonary vascular bed is perfused by large SCAs, proliferative lesions like those found in large left-to-right shunts may occur. Early in infancy, banding of large, nonstenotic SCAs could protect the distal pulmonary vasculature. Moreover, total surgical repair should be associated with ligation of the SCA to avoid residual left-to-right shunt, if the pulmonary arteries can carry the full pulmonary blood flow.", "contents": "Histology of pulmonary arterial supply in pulmonary atresia with ventricular septal defect. A histologic study was performed on 22 specimens of pulmonary atresia with ventricular septal defect to 1) ascertain the existence of the main pulmonary artery; 2) distinguish the ductus arteriosus from the systemic collateral arteries (SCA); 3) establish the nutritive or functional nature of collateral circulation; and 4) evaluate the morphology of the distal pulmonary bed. Three cases had absent main pulmonary artery, one with and two without signs of infundibular septation. We suggest that absent main pulmonary artery may exist with both infundibular pulmonary atresia and persistent truncus arteriosus. SCAs have been found to have similar histological features as systemic muscular arteries of the same size--their medial muscular layer merges gradually into an elastic one at different depth inside the lungs. Injection of contrast material allowed us to demonstrate that these vessels are functional, since they inosculate into efficient pulmonary arteries ending in the respiratory units. When the distal pulmonary vascular bed is perfused by large SCAs, proliferative lesions like those found in large left-to-right shunts may occur. Early in infancy, banding of large, nonstenotic SCAs could protect the distal pulmonary vasculature. Moreover, total surgical repair should be associated with ligation of the SCA to avoid residual left-to-right shunt, if the pulmonary arteries can carry the full pulmonary blood flow."}
{"id": "PMID:487541", "title": "Effects of acute hemodynamic alterations on pulmonic valve motion. Experimental and clinical echocardiographic studies.", "content": "The purpose of this study was to assess the effects of acute alterations of the pulmonary circulation on the pulmonic valve echocardiogram. We measured the pulmonic valve opening velocity (PVOV) and right-sided systolic time intervals (right ventricular preejection period-to-right ventricular ejection time ratio [RPEP/RVET] in 22 open-chest dogs subjected to acute hemodynamic alterations produced by inferior vena cava constriction, atrial pacing, isoproterenol infusion and microsphere embolization of the pulmonary artery. We found only fair correlations between PVOV and peak pulmonary artery flow (r = 0.56), right ventricular dp/dt (r = 0.43), stroke volume (r = 0.42), pulmonary artery systolic pressure (r = 0.33) and peak pulmonary artery acceleration (r = 0.31). RVET was shortened by reduced venous return (caval constriction) and by increases in heart rate (atrial pacing and isoproterenol), which resulted in increases in RPEP/RVET that did not correspond well to simultaneous changes in pulmonary artery pressure. In seven patients breathing 10% O2 to raise pulmonary artery pressure acutely, we found no change or a fall in PVOV. Thus, the pulmonic valve echocardiogram is influenced by multiple factors relating to parameters of pulmonary flow and right ventricular contractility, and may be of limited clinical usefulness in predicting pulmonary artery pressures.", "contents": "Effects of acute hemodynamic alterations on pulmonic valve motion. Experimental and clinical echocardiographic studies. The purpose of this study was to assess the effects of acute alterations of the pulmonary circulation on the pulmonic valve echocardiogram. We measured the pulmonic valve opening velocity (PVOV) and right-sided systolic time intervals (right ventricular preejection period-to-right ventricular ejection time ratio [RPEP/RVET] in 22 open-chest dogs subjected to acute hemodynamic alterations produced by inferior vena cava constriction, atrial pacing, isoproterenol infusion and microsphere embolization of the pulmonary artery. We found only fair correlations between PVOV and peak pulmonary artery flow (r = 0.56), right ventricular dp/dt (r = 0.43), stroke volume (r = 0.42), pulmonary artery systolic pressure (r = 0.33) and peak pulmonary artery acceleration (r = 0.31). RVET was shortened by reduced venous return (caval constriction) and by increases in heart rate (atrial pacing and isoproterenol), which resulted in increases in RPEP/RVET that did not correspond well to simultaneous changes in pulmonary artery pressure. In seven patients breathing 10% O2 to raise pulmonary artery pressure acutely, we found no change or a fall in PVOV. Thus, the pulmonic valve echocardiogram is influenced by multiple factors relating to parameters of pulmonary flow and right ventricular contractility, and may be of limited clinical usefulness in predicting pulmonary artery pressures."}
{"id": "PMID:487543", "title": "Noninvasive assessment of atrioventricular pressure half-time by Doppler ultrasound.", "content": "The mean pressure drop across the mitral valve and atrioventricular pressure half-time were measured noninvasively by Doppler ultrasound in 40 normal subjects, in 17 patients with mitral regurgitation, 32 patients with mitral stenosis and 12 with combined stenosis and regurgitation. In normal subjects pressure half-times were 20--60 msec, in patients with isolated mitral regurgitation 35--80 msec and in patients with mitral stenosis 90--383 msec. There was no significant change in pressure half-time with exercise or on repeat examinations, indicating relative independence of mitral flow. In 25 patients with mitral stenosis and seven with combined stenosis and regurgitation, pressure half-time was related to mitral valve area calculated from catheterization data. Increasing pressure half-times occurred with decreasing mitral valve area, and this relationship was not influenced by additional mitral regurgitation. Noninvasive measurement of pressure half-time together with mean pressure drop was useful for evaluating patients with mitral valve disease.", "contents": "Noninvasive assessment of atrioventricular pressure half-time by Doppler ultrasound. The mean pressure drop across the mitral valve and atrioventricular pressure half-time were measured noninvasively by Doppler ultrasound in 40 normal subjects, in 17 patients with mitral regurgitation, 32 patients with mitral stenosis and 12 with combined stenosis and regurgitation. In normal subjects pressure half-times were 20--60 msec, in patients with isolated mitral regurgitation 35--80 msec and in patients with mitral stenosis 90--383 msec. There was no significant change in pressure half-time with exercise or on repeat examinations, indicating relative independence of mitral flow. In 25 patients with mitral stenosis and seven with combined stenosis and regurgitation, pressure half-time was related to mitral valve area calculated from catheterization data. Increasing pressure half-times occurred with decreasing mitral valve area, and this relationship was not influenced by additional mitral regurgitation. Noninvasive measurement of pressure half-time together with mean pressure drop was useful for evaluating patients with mitral valve disease."}
{"id": "PMID:487544", "title": "Cross-sectional echocardiography. I. Analysis of mathematic models for quantifying mass of the left ventricle in dogs.", "content": "Cross-sectional echocardiography was used to quantify left ventricular mass noninvasively in 21 dogs. Short- and long-axis cross-sectional images of the left ventricle were reproducibly traced at endocardial and epicardial borders during stop-motion video-tape replay. We used area, length and diameter measurements to calculate left ventricular mass by seven mathematic models, including the standard formulas used with M-mode echocardiography and cineangiography. Calculated mass was compared with excised weight of the left ventricle by regression and percent error analyses. Formulas using short-axis areas and long-axis length resulted in higher correlation coefficients (0.94--0.95) and lower mean errors (6--7%) than for standard formulas. Since short-axis areas account for regional left ventricular irregularities, noninvasive quantification of left ventricular mass by cross-sectional echocardiography in dogs is most accurate with formulas using short-axis areas.", "contents": "Cross-sectional echocardiography. I. Analysis of mathematic models for quantifying mass of the left ventricle in dogs. Cross-sectional echocardiography was used to quantify left ventricular mass noninvasively in 21 dogs. Short- and long-axis cross-sectional images of the left ventricle were reproducibly traced at endocardial and epicardial borders during stop-motion video-tape replay. We used area, length and diameter measurements to calculate left ventricular mass by seven mathematic models, including the standard formulas used with M-mode echocardiography and cineangiography. Calculated mass was compared with excised weight of the left ventricle by regression and percent error analyses. Formulas using short-axis areas and long-axis length resulted in higher correlation coefficients (0.94--0.95) and lower mean errors (6--7%) than for standard formulas. Since short-axis areas account for regional left ventricular irregularities, noninvasive quantification of left ventricular mass by cross-sectional echocardiography in dogs is most accurate with formulas using short-axis areas."}
{"id": "PMID:487546", "title": "Scintigraphic characteristics of experimental myocardial infarct extension.", "content": "Technetium-99m-stannous pyrophosphate scintiphotos were evaluated for diagnosing and quantitating myocardial infarct (MI) extension in sedated dogs. Infarction and extension were produced by serial left anterior descending coronary artery ligations at 0 and 48 hours. We compared serial scintiphoto data with regional myocardial blood flow (MBF) (microsphere technique) and infarct histopathology. In eight control dogs, the scintigraphic MI area was stable at 24, 48, and 72 hours. In each of 11 dogs undergoing extension, the MI area increased after the 48-hour occlusion, averaging a 48.9% increase (p less than 0.001). Grossly, most extensions were mixtures of confluent necrosis and moderate (patchy) necrosis. MBF to confluent infarct tissue decreased significantly, allowing the documentation of extension by totaling the grams of newly flow-deprived tissue, but patchy infarct tissue had little flow deprivation, making it difficult to quantitate this type of extension accurately by flow criteria alone. Rarely, extension could be diagnosed using conventional histologic criteria. We concluded that the scintiphoto MI area was related quantitatively to infarct weight in both control and extension. However, it was not possible to determine that an increase in the MI scintiphoto area was an accurate predictor of the degree of extension using independent flow or pathologic criteria.", "contents": "Scintigraphic characteristics of experimental myocardial infarct extension. Technetium-99m-stannous pyrophosphate scintiphotos were evaluated for diagnosing and quantitating myocardial infarct (MI) extension in sedated dogs. Infarction and extension were produced by serial left anterior descending coronary artery ligations at 0 and 48 hours. We compared serial scintiphoto data with regional myocardial blood flow (MBF) (microsphere technique) and infarct histopathology. In eight control dogs, the scintigraphic MI area was stable at 24, 48, and 72 hours. In each of 11 dogs undergoing extension, the MI area increased after the 48-hour occlusion, averaging a 48.9% increase (p less than 0.001). Grossly, most extensions were mixtures of confluent necrosis and moderate (patchy) necrosis. MBF to confluent infarct tissue decreased significantly, allowing the documentation of extension by totaling the grams of newly flow-deprived tissue, but patchy infarct tissue had little flow deprivation, making it difficult to quantitate this type of extension accurately by flow criteria alone. Rarely, extension could be diagnosed using conventional histologic criteria. We concluded that the scintiphoto MI area was related quantitatively to infarct weight in both control and extension. However, it was not possible to determine that an increase in the MI scintiphoto area was an accurate predictor of the degree of extension using independent flow or pathologic criteria."}
{"id": "PMID:487548", "title": "Evaluation of transthoracic electrical impedance in the diagnosis of pulmonary edema.", "content": "To evaluate the clinical usefulness of measuring transthoracic electrical impedance in patients with pulmonary edema, we studied 27 normal subjects and 33 patients. In normal subjects, impedance increased when body position changed from supine to standing (p less than 0.01) and when lung volume increased from residual volume to total lung capacity (p less than 0.01). Impedance values vari7 omega) when measured hourly without removing the electrodes. In only five of 11 patients with clinically severe pulmonary edema and five of eight with radiologically severe pulmonary edema were impedance values outside the normal range. Changes in impedance values correlated with changes in clinical and radiographic indexes of pulmonary edema. We conclude that (1) single measurements of impedance are useless in diagnosing pulmonary edema, and (2) impedance changes as predicted as pulmonary edema increases and decreases, and these changes are readily detected by simpler methods of evaluation.", "contents": "Evaluation of transthoracic electrical impedance in the diagnosis of pulmonary edema. To evaluate the clinical usefulness of measuring transthoracic electrical impedance in patients with pulmonary edema, we studied 27 normal subjects and 33 patients. In normal subjects, impedance increased when body position changed from supine to standing (p less than 0.01) and when lung volume increased from residual volume to total lung capacity (p less than 0.01). Impedance values vari7 omega) when measured hourly without removing the electrodes. In only five of 11 patients with clinically severe pulmonary edema and five of eight with radiologically severe pulmonary edema were impedance values outside the normal range. Changes in impedance values correlated with changes in clinical and radiographic indexes of pulmonary edema. We conclude that (1) single measurements of impedance are useless in diagnosing pulmonary edema, and (2) impedance changes as predicted as pulmonary edema increases and decreases, and these changes are readily detected by simpler methods of evaluation."}
{"id": "PMID:487549", "title": "Two-dimensional echocardiographic demonstration of left atrial thrombi in patients with prosthetic mitral valves.", "content": "Although M-mode echocardiography (MME) is not a reliable method for detecting left atrial thrombi, recent reports suggest that two-dimensional echo (2DE) may be more effective than MME in identifying intracardiac thrombi. In three patients with prosthetic mitral valves who presented with either arterial embolization or prosthetic valvular dysfunction, 2DE demonstrated left atrial masses consistent with thrombi, while MME was either negative (two patients) or suspicious (one patient) for left atrial thrombus. Thrombi were documented by surgical or postmortem examination in all cases. Clear delineation of the atrial cavity and the margins of the masses, visualization on multiple echocardiographic views and comparison of serial examinations were helpful in identifying these masses as thrombi. In addition, the masses visualized had certain patterns of motion which seem unique and may allow characterization of atrial masses as thrombi.", "contents": "Two-dimensional echocardiographic demonstration of left atrial thrombi in patients with prosthetic mitral valves. Although M-mode echocardiography (MME) is not a reliable method for detecting left atrial thrombi, recent reports suggest that two-dimensional echo (2DE) may be more effective than MME in identifying intracardiac thrombi. In three patients with prosthetic mitral valves who presented with either arterial embolization or prosthetic valvular dysfunction, 2DE demonstrated left atrial masses consistent with thrombi, while MME was either negative (two patients) or suspicious (one patient) for left atrial thrombus. Thrombi were documented by surgical or postmortem examination in all cases. Clear delineation of the atrial cavity and the margins of the masses, visualization on multiple echocardiographic views and comparison of serial examinations were helpful in identifying these masses as thrombi. In addition, the masses visualized had certain patterns of motion which seem unique and may allow characterization of atrial masses as thrombi."}
{"id": "PMID:487556", "title": "Computer diagnosis of supraventricular and ventricular arrhythmias. A new esophageal technique.", "content": "Computerized arrhythmia monitors recognize only a few of the significant arrhythmias and generally fail to detect arrhythmias of supraventricular origin. This is because conventional surface leads, which are sufficient for QRS recognition, are highly inadequate for automated P-wave detection. A new two-lead system, which includes a swallowable capsule-electrode for esophageal monitoring of atrial activity, is used in an on-line arrhythmia monitor. Three interval measurements (AA, AR and RR) and a QRS shape measurement provide the foundation for a detailed interpretation of each beat. Building on the single-beat analysis, a contextual diagnostic algorithm then recognizes and reports on-line the following arrhythmias: couplets, bigeminy, trigeminy, ventricular tachycardia, supraventricular tachycardia, atrial flutter, atrial fibrillation, ventricular tachycardia with retrograde conduction to the atria, first-degree block, second-degree block, Wenckebach periodicity, advanced block, third-degree block and sinus bradycardia.", "contents": "Computer diagnosis of supraventricular and ventricular arrhythmias. A new esophageal technique. Computerized arrhythmia monitors recognize only a few of the significant arrhythmias and generally fail to detect arrhythmias of supraventricular origin. This is because conventional surface leads, which are sufficient for QRS recognition, are highly inadequate for automated P-wave detection. A new two-lead system, which includes a swallowable capsule-electrode for esophageal monitoring of atrial activity, is used in an on-line arrhythmia monitor. Three interval measurements (AA, AR and RR) and a QRS shape measurement provide the foundation for a detailed interpretation of each beat. Building on the single-beat analysis, a contextual diagnostic algorithm then recognizes and reports on-line the following arrhythmias: couplets, bigeminy, trigeminy, ventricular tachycardia, supraventricular tachycardia, atrial flutter, atrial fibrillation, ventricular tachycardia with retrograde conduction to the atria, first-degree block, second-degree block, Wenckebach periodicity, advanced block, third-degree block and sinus bradycardia."}
{"id": "PMID:487557", "title": "Ventricular tachycardia and ventricular fibrillation in a young population.", "content": "In this study, we describe the findings in 18 young patients (age range 4 days to 24 years, mean 16.6 years) who had ventricular tachycardia and/or ventricular fibrillation and were followed for 4--70 months (mean 22.4 months). Patients had a variety of problems associated with their arrhythmia, including mitral valve prolapse, cardiomyopathy, myocarditis, prolonged QT syndrome and hypokalemia. Six patients had no clinically recognizable cardiac abnormality. The ventricular tachycardia showed a left bundle branch block contour in 10 of 17 patients, right bundle branch block in four, was multiform in two and had an indeterminate contour in one. Sustained ventricular tachycardia was initiated and terminated reproducibly by atrial and ventricular stimulation in three of seven patients who did not have spontaneous episodes of ventricular tachycardia during the electrophysiologic study. In one other patient, short bursts of ventricular tachycardia were induced. Patients who had ventricular fibrillation, those who died, and those who are still symptomatic with poorly controlled ventricular arrhythmias had significant heart disease. In one patient, a ventricular tachyarrhythmia that had required more than 100 electrical cardioversions spontaneously disappeared after requiring 1 year of antiarrhythmic therapy.", "contents": "Ventricular tachycardia and ventricular fibrillation in a young population. In this study, we describe the findings in 18 young patients (age range 4 days to 24 years, mean 16.6 years) who had ventricular tachycardia and/or ventricular fibrillation and were followed for 4--70 months (mean 22.4 months). Patients had a variety of problems associated with their arrhythmia, including mitral valve prolapse, cardiomyopathy, myocarditis, prolonged QT syndrome and hypokalemia. Six patients had no clinically recognizable cardiac abnormality. The ventricular tachycardia showed a left bundle branch block contour in 10 of 17 patients, right bundle branch block in four, was multiform in two and had an indeterminate contour in one. Sustained ventricular tachycardia was initiated and terminated reproducibly by atrial and ventricular stimulation in three of seven patients who did not have spontaneous episodes of ventricular tachycardia during the electrophysiologic study. In one other patient, short bursts of ventricular tachycardia were induced. Patients who had ventricular fibrillation, those who died, and those who are still symptomatic with poorly controlled ventricular arrhythmias had significant heart disease. In one patient, a ventricular tachyarrhythmia that had required more than 100 electrical cardioversions spontaneously disappeared after requiring 1 year of antiarrhythmic therapy."}
{"id": "PMID:487558", "title": "Ventricular ectopic beats and their relation to sudden and nonsudden cardiac death after myocardial infarction.", "content": "The role of ventricular ectopic beats (VEBs) in identifying patients who die of cardiac cause in the posthospital phase of myocardial infarction was evaluated in 940 patients who survived an acute coronary event. Six-hour Holter ECG recordings were obtained before hospital discharge, and VEBs were classified as complex (bigeminal, multiform, repetitive or R on T), simple (one or more VEBs that did not have complex patterns), or not present. Patients were followed 1-60 months (average 36 months) and cardiac mortality was categorized as sudden (less than or equal to 1 hour) or nonsudden (greater than 1 hour) among 98 witnessed cardiac deaths. Complex VEBs were associated with a significantly increased cardiac death rate, but did not discriminate between sudden and nonsudden death. Simple VEBs were associated with a 3-year cardiac mortality rate intermediate between those with complex and those with no VEBs. The relationship between complex VEBs and cardiac mortality was independent of 10 relevant clinical variables.", "contents": "Ventricular ectopic beats and their relation to sudden and nonsudden cardiac death after myocardial infarction. The role of ventricular ectopic beats (VEBs) in identifying patients who die of cardiac cause in the posthospital phase of myocardial infarction was evaluated in 940 patients who survived an acute coronary event. Six-hour Holter ECG recordings were obtained before hospital discharge, and VEBs were classified as complex (bigeminal, multiform, repetitive or R on T), simple (one or more VEBs that did not have complex patterns), or not present. Patients were followed 1-60 months (average 36 months) and cardiac mortality was categorized as sudden (less than or equal to 1 hour) or nonsudden (greater than 1 hour) among 98 witnessed cardiac deaths. Complex VEBs were associated with a significantly increased cardiac death rate, but did not discriminate between sudden and nonsudden death. Simple VEBs were associated with a 3-year cardiac mortality rate intermediate between those with complex and those with no VEBs. The relationship between complex VEBs and cardiac mortality was independent of 10 relevant clinical variables."}
{"id": "PMID:487559", "title": "Motilin, human pancreatic polypeptide (HPP) and gastrin plasma concentrations in fasting subjects.", "content": "Motilin, human pancreatic polypeptide (HPP) and gastrin plasma concentrations have been determined in 196 fasting subjects ranging in age from the third to ninth decade. Detailed descriptions of the motilin, HPP and gastrin radioimmunoassays are presented. Plasma motilin concentrations ranged from undetectable to 1253 ng/L with a median value at 157 ng/L. Age and sex did not influence plasma motilin concentrations. Plasma HPP concentrations ranged from undetectable to 1564 ng/L with a median value at 142 ng/L. Plasma HPP concentrations increased significantly with age; males generally had higher concentrations than females. Plasma gastrin concentrations ranged from undetectable to 1658 ng/L with a median value of 21 ng/L. Age and sex did not influence plasma concentrations. These data establish ranges for plasma motilin, HPP and gastrin in fasting subjects aged 21--90 years using the described radioimmunoassays.", "contents": "Motilin, human pancreatic polypeptide (HPP) and gastrin plasma concentrations in fasting subjects. Motilin, human pancreatic polypeptide (HPP) and gastrin plasma concentrations have been determined in 196 fasting subjects ranging in age from the third to ninth decade. Detailed descriptions of the motilin, HPP and gastrin radioimmunoassays are presented. Plasma motilin concentrations ranged from undetectable to 1253 ng/L with a median value at 157 ng/L. Age and sex did not influence plasma motilin concentrations. Plasma HPP concentrations ranged from undetectable to 1564 ng/L with a median value at 142 ng/L. Plasma HPP concentrations increased significantly with age; males generally had higher concentrations than females. Plasma gastrin concentrations ranged from undetectable to 1658 ng/L with a median value of 21 ng/L. Age and sex did not influence plasma concentrations. These data establish ranges for plasma motilin, HPP and gastrin in fasting subjects aged 21--90 years using the described radioimmunoassays."}
{"id": "PMID:487560", "title": "Evaluation of serum digoxin and serum cortisol by radioimmunoassay with the Concept-4.", "content": "The solid-phase radioimmunoassay procedures for serum digoxin and cortisol with the Concept-4 were evaluated for specificity, correlation with other radioimmunoassay procedures, parallelism, recovery, carryover, and precision. 1. The detection limits of the digoxin and cortisol assays were 10 pg/tube and 40 pg/tube, respectively. These detections correspond to serum concentrations of 0.25 micrograms/liter and 20 micrograms/liter, respectively, for digoxin and cortisol. 2. Specificity of the digoxin assay was characterized by a 18.3% cross reaction with digitoxin. The anti-cortisol with 11-deoxycortisol, cross reaction with 11-deoxycortisol. 3. The values obtained with the Concept-4 for both procedures demonstrated acceptable correlations with other existing RIA procedures. 4. Dilution studies conducted to determine the parallelism of the 2 assays indicated that both assays demonstrated excellent proportionality between the volume of specimen and the quantity of analyte measured. 5. Analytical recoveries of digoxin and cortisol added to the individual assays average 95% and 101%, respectively. 6. Carryover studies indicated a routine sample-to-sample interaction of less than 0.05% for both assays. 7. The digoxin assay routinely demonstrated intra- and inter-assay variations of less than 7.4% and less than 10.0%, respectively. The precision data for cortisol indicated intra- and inter-assay variations of less than 6.7% and less than 9.1%, respectively.", "contents": "Evaluation of serum digoxin and serum cortisol by radioimmunoassay with the Concept-4. The solid-phase radioimmunoassay procedures for serum digoxin and cortisol with the Concept-4 were evaluated for specificity, correlation with other radioimmunoassay procedures, parallelism, recovery, carryover, and precision. 1. The detection limits of the digoxin and cortisol assays were 10 pg/tube and 40 pg/tube, respectively. These detections correspond to serum concentrations of 0.25 micrograms/liter and 20 micrograms/liter, respectively, for digoxin and cortisol. 2. Specificity of the digoxin assay was characterized by a 18.3% cross reaction with digitoxin. The anti-cortisol with 11-deoxycortisol, cross reaction with 11-deoxycortisol. 3. The values obtained with the Concept-4 for both procedures demonstrated acceptable correlations with other existing RIA procedures. 4. Dilution studies conducted to determine the parallelism of the 2 assays indicated that both assays demonstrated excellent proportionality between the volume of specimen and the quantity of analyte measured. 5. Analytical recoveries of digoxin and cortisol added to the individual assays average 95% and 101%, respectively. 6. Carryover studies indicated a routine sample-to-sample interaction of less than 0.05% for both assays. 7. The digoxin assay routinely demonstrated intra- and inter-assay variations of less than 7.4% and less than 10.0%, respectively. The precision data for cortisol indicated intra- and inter-assay variations of less than 6.7% and less than 9.1%, respectively."}
{"id": "PMID:487561", "title": "Investigation of reaction intermediates of the urea-diacetylmonoxime reaction.", "content": "1. An investigation of the intermediates of the urea-diacetylmonoxime reaction and the isolation of the protochromogen has been described. A comparative spectral study of the protochromogen with that of the literature values suggested the structure of the protochromogen to be a diureide. 2. The diureides of 1-phenyl-1,2-propanedione-monoxime and 2,3-pentanedione were synthesized and their visible, U.V., I.R., NMR, and mass desorption spectrometry data were analyzed. The study suggests that the structure of each of these compound is that of a diureide. When these diureides were redissolved in acid media, they gave the color expected of the corresponding chromogens found from the respective diketones and urea. 3. The 3a-methyl-6a-phenyl-glycoluril was synthesized by a base-catalyzed reaction. When this glycoluril was dissolved in concentrated HCl, a pink color was obtained, similar to that of the corresponding diureide. These studies indicate that both the diureide and the glycoluril (if formed at all in acid-catalyzed reaction) are converted to the same chromogen, possibly via a rearrangement. 4. When 2,3-butanedionemonoximethiosemicarbazone was reacted with urea in acid medium, characteristic spectral data were obtained which were very similar to those of the urea-diacetylmonoximethiosemicarbazide reaction. This tends to indicate that the possible structures of the protochromogens are similar and of the open chain diureide type.", "contents": "Investigation of reaction intermediates of the urea-diacetylmonoxime reaction. 1. An investigation of the intermediates of the urea-diacetylmonoxime reaction and the isolation of the protochromogen has been described. A comparative spectral study of the protochromogen with that of the literature values suggested the structure of the protochromogen to be a diureide. 2. The diureides of 1-phenyl-1,2-propanedione-monoxime and 2,3-pentanedione were synthesized and their visible, U.V., I.R., NMR, and mass desorption spectrometry data were analyzed. The study suggests that the structure of each of these compound is that of a diureide. When these diureides were redissolved in acid media, they gave the color expected of the corresponding chromogens found from the respective diketones and urea. 3. The 3a-methyl-6a-phenyl-glycoluril was synthesized by a base-catalyzed reaction. When this glycoluril was dissolved in concentrated HCl, a pink color was obtained, similar to that of the corresponding diureide. These studies indicate that both the diureide and the glycoluril (if formed at all in acid-catalyzed reaction) are converted to the same chromogen, possibly via a rearrangement. 4. When 2,3-butanedionemonoximethiosemicarbazone was reacted with urea in acid medium, characteristic spectral data were obtained which were very similar to those of the urea-diacetylmonoximethiosemicarbazide reaction. This tends to indicate that the possible structures of the protochromogens are similar and of the open chain diureide type."}
{"id": "PMID:487562", "title": "The effect of pre-incubation and direct procedure for the alanine transaminase assay in two kits compared.", "content": "The kinetic measurement of serum alanine transaminase was carried out on two enzyme kits (Roche and Calbiochem), done as a direct method and a pre-incubation method. Variable results from commercial control sera and patient sera by these four procedures were obtained. Pre-incubation enzyme values were generally lower than the direct values (mean difference 10%), but there were marked differences between the results from the two kits. This finding has important significance especially for laboratories participating in external QC Programmes. Frequent precision checks of the method and a local reference range are therefore necessary.", "contents": "The effect of pre-incubation and direct procedure for the alanine transaminase assay in two kits compared. The kinetic measurement of serum alanine transaminase was carried out on two enzyme kits (Roche and Calbiochem), done as a direct method and a pre-incubation method. Variable results from commercial control sera and patient sera by these four procedures were obtained. Pre-incubation enzyme values were generally lower than the direct values (mean difference 10%), but there were marked differences between the results from the two kits. This finding has important significance especially for laboratories participating in external QC Programmes. Frequent precision checks of the method and a local reference range are therefore necessary."}
{"id": "PMID:487563", "title": "Serum calcium ion activity. Some aspects on methodological differences and intraindividual variation.", "content": "Sera were analyzed for calcium ion activity on the Orion 99-20 and SS-20 systems. Samples from 84 healthy subjects were analyzed on both systems and, because of a change of electrode composition, 74 of them were reanalyzed after this change. Additional 89 were analyzed on only SS-20. The distribution of serum calcium ion activity was close to Gaussian. The difference between the 99-20 and SS-20 readings was 0.048+/-0.017 mmol/liter (range -0.01 to 0.08) before the electrode change and -0.085+/-0.027 mmol/liter (range -0.14 to 0.02) after. The reference ranges were 1.02--1.23 mmol/liter for 99-20 and 0.93--1.20 mmol/liter for SS-20 before the electrode change. The following characteristics may contribute to differences in the readings: higher temperature, higher flow rate, new calcium and reference electrodes, new liquid junction construction and a new pumping procedure. For 74 subjects the determination of serum calcium ion activity (99-20 system) was performed with a five year interval and the difference between the first and second measurements was -0.0046+/-0.0168 mmol/liter (mean +/-SD), ranging from -0.04 to 0.03 mmol/liter. This corresponded to an intraindividual variation of 1.5%. The corresponding variations for serum total calcium and corrected calcium were 4.8% and 3.3% respectively.", "contents": "Serum calcium ion activity. Some aspects on methodological differences and intraindividual variation. Sera were analyzed for calcium ion activity on the Orion 99-20 and SS-20 systems. Samples from 84 healthy subjects were analyzed on both systems and, because of a change of electrode composition, 74 of them were reanalyzed after this change. Additional 89 were analyzed on only SS-20. The distribution of serum calcium ion activity was close to Gaussian. The difference between the 99-20 and SS-20 readings was 0.048+/-0.017 mmol/liter (range -0.01 to 0.08) before the electrode change and -0.085+/-0.027 mmol/liter (range -0.14 to 0.02) after. The reference ranges were 1.02--1.23 mmol/liter for 99-20 and 0.93--1.20 mmol/liter for SS-20 before the electrode change. The following characteristics may contribute to differences in the readings: higher temperature, higher flow rate, new calcium and reference electrodes, new liquid junction construction and a new pumping procedure. For 74 subjects the determination of serum calcium ion activity (99-20 system) was performed with a five year interval and the difference between the first and second measurements was -0.0046+/-0.0168 mmol/liter (mean +/-SD), ranging from -0.04 to 0.03 mmol/liter. This corresponded to an intraindividual variation of 1.5%. The corresponding variations for serum total calcium and corrected calcium were 4.8% and 3.3% respectively."}
{"id": "PMID:487567", "title": "Further studies on the electrophoretic pattern of albumin in diabetic sera.", "content": "Human sera from normal subjects and diabetic patients were electrophoresed in an urea-containing gel; polyacrylamide gel and cellulose acetate membrane. In polyacrylamide gel, normal serum exhibited the fast migrating Band 2 and the slower migrating albumin Bands 4 and 5 after overnight fasting and also after glucose administration. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration in low mercaptoethanol gel which abnormality resembled the pattern in C57BL/KsJ-db/db mice. In high mercaptoethanol gel, about half of the diabetic sera exhibit a delay in appearance of Bands 4 and 5, i.e., Bands 4 and 5 were not observed 30--60 min after glucose administration, which seems to resemble the pattern in C57BL/6J-ob/ob mice. Conditions of electrophoresis in urea-submerged cellulose acetate membrane (species of buffer systems, pH, ion concentration, mercaptoethanol, ethylene-diaminetetraacetic acid, Ca2+ ion, urea concentration, etc.) were observed in relation to albumin sub-separation. At pH 8.6 with barbital buffer, albumin separated into two bands, and at pH 10.6 with glycine buffer, albumin separated into four bands. Almost all diabetic sera (ca. 80%) exhibited different electrophoretic patterns from that of normal serum.", "contents": "Further studies on the electrophoretic pattern of albumin in diabetic sera. Human sera from normal subjects and diabetic patients were electrophoresed in an urea-containing gel; polyacrylamide gel and cellulose acetate membrane. In polyacrylamide gel, normal serum exhibited the fast migrating Band 2 and the slower migrating albumin Bands 4 and 5 after overnight fasting and also after glucose administration. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration in low mercaptoethanol gel which abnormality resembled the pattern in C57BL/KsJ-db/db mice. In high mercaptoethanol gel, about half of the diabetic sera exhibit a delay in appearance of Bands 4 and 5, i.e., Bands 4 and 5 were not observed 30--60 min after glucose administration, which seems to resemble the pattern in C57BL/6J-ob/ob mice. Conditions of electrophoresis in urea-submerged cellulose acetate membrane (species of buffer systems, pH, ion concentration, mercaptoethanol, ethylene-diaminetetraacetic acid, Ca2+ ion, urea concentration, etc.) were observed in relation to albumin sub-separation. At pH 8.6 with barbital buffer, albumin separated into two bands, and at pH 10.6 with glycine buffer, albumin separated into four bands. Almost all diabetic sera (ca. 80%) exhibited different electrophoretic patterns from that of normal serum."}
{"id": "PMID:487568", "title": "Electrolytes and norepinephrine levels in blood of patients with cystic fibrosis.", "content": "Young adults with cystic fibrosis in good to excellent condition have reduced plasma sodium and chloride and elevated plasma potassium compared to a group of healthy young adult control subjects. Blood pressure was also lower in the patients with cystic fibrosis. However, plasma norepinephrine and dopamine-beta-hydroxylase and their response to standing and isometric hand grip were normal in the cystic fibrosis patients.", "contents": "Electrolytes and norepinephrine levels in blood of patients with cystic fibrosis. Young adults with cystic fibrosis in good to excellent condition have reduced plasma sodium and chloride and elevated plasma potassium compared to a group of healthy young adult control subjects. Blood pressure was also lower in the patients with cystic fibrosis. However, plasma norepinephrine and dopamine-beta-hydroxylase and their response to standing and isometric hand grip were normal in the cystic fibrosis patients."}
{"id": "PMID:487569", "title": "Short chain fatty acids in plasma and brain: quantitative determination by gas chromatography.", "content": "A reliable and practicable method for the determination of short chain fatty acids in plasma and brain tissue is presented. The sample preparation by partition chromatography on silicic acid allows subsequently a quantitation of short chain fatty acids without interference by methylmalonic acid, or other more polar compounds. With the gas-chromatographic system 2-methylbutyrate is separated from isovalerate. Reference values are given for plasma. The system is also useful in combination with mass spectrometry.", "contents": "Short chain fatty acids in plasma and brain: quantitative determination by gas chromatography. A reliable and practicable method for the determination of short chain fatty acids in plasma and brain tissue is presented. The sample preparation by partition chromatography on silicic acid allows subsequently a quantitation of short chain fatty acids without interference by methylmalonic acid, or other more polar compounds. With the gas-chromatographic system 2-methylbutyrate is separated from isovalerate. Reference values are given for plasma. The system is also useful in combination with mass spectrometry."}
{"id": "PMID:487570", "title": "Plasma and white blood cell ascorbic acid concentrations in patients with bronchial asthma.", "content": "Plasma cholesterol, plasma and white blood cell ascorbic acid concentrations were estimated in 30 untreated, 32 salbutamol-treated patients with bronchial asthma and in 57 normal controls. Treated and untreated asthmatics had significantly higher plasma cholesterol but lower plasma and white cell ascorbic acid levels than normal controls. A negative correlation was found between white cell ascorbic acid level and plasma cholesterol concentration in both treated and untreated asthmatics. These findings are discussed.", "contents": "Plasma and white blood cell ascorbic acid concentrations in patients with bronchial asthma. Plasma cholesterol, plasma and white blood cell ascorbic acid concentrations were estimated in 30 untreated, 32 salbutamol-treated patients with bronchial asthma and in 57 normal controls. Treated and untreated asthmatics had significantly higher plasma cholesterol but lower plasma and white cell ascorbic acid levels than normal controls. A negative correlation was found between white cell ascorbic acid level and plasma cholesterol concentration in both treated and untreated asthmatics. These findings are discussed."}
{"id": "PMID:487571", "title": "A highly specific 125I-radioimmunoassay for cholic acid conjugates.", "content": "Several modifications of the immunization procedure permitted development of a highly specific radioimmunoassay (RIA) for cholic acid conjugates. Antiserum was produced in guinea pigs using cholic acid-thyroglobulin complex as immunogen. 125-I-Cholyglycylhistamine was prepared as radioactive ligand according to a modification of the method of Spenney et al. (Spenney, J.G., Johnson, B.J., Hirschowitz, B.I., Mihas, A.A. and Gibson, R. (1977) Gastroenterology 72, 305--311). The association constant of the antisera to taurocholic acid was 1.8 x 10(7) l/mol, the working range of the assay between 9.5--890 pmol. Cross-reactivities of the antiserum to bile acids other than cholic acid species were less than 3%, which is lower than for any published bile acid RIA. Concentrations of cholic acid conjugates in sera obtained from 17 healthy fasting volunteers ranged from 0.4--1.9/mumul/l.", "contents": "A highly specific 125I-radioimmunoassay for cholic acid conjugates. Several modifications of the immunization procedure permitted development of a highly specific radioimmunoassay (RIA) for cholic acid conjugates. Antiserum was produced in guinea pigs using cholic acid-thyroglobulin complex as immunogen. 125-I-Cholyglycylhistamine was prepared as radioactive ligand according to a modification of the method of Spenney et al. (Spenney, J.G., Johnson, B.J., Hirschowitz, B.I., Mihas, A.A. and Gibson, R. (1977) Gastroenterology 72, 305--311). The association constant of the antisera to taurocholic acid was 1.8 x 10(7) l/mol, the working range of the assay between 9.5--890 pmol. Cross-reactivities of the antiserum to bile acids other than cholic acid species were less than 3%, which is lower than for any published bile acid RIA. Concentrations of cholic acid conjugates in sera obtained from 17 healthy fasting volunteers ranged from 0.4--1.9/mumul/l."}
{"id": "PMID:487572", "title": "Urinary dihydroxanthopterin in the diagnosis of malignant hyperphenylalaninemia and phenylketonuria.", "content": "Assessment of urinary dihydroxanthopterin is proposed as a simple method of recognition of patients with malignant hyperphenylalaninemia (MHPA). High levels of urinary dihydroxanthopterin are found in untreated patients with phenylketonuria (PKU) or with dihydropteridine reductase (DHPR) deficiency. After dietary control of the serum phenylalanine level in PKU, the urinary dihydroxanthopterin falls to near normal levels. In DHPR deficiency urinary dihydroxanthopterin levels are high even when serum phenylalanine levels are in the range achieved on dietary treatment. Low levels would be expected in patients with defects in tetrahydrobiopterin synthesis even before dietary treatment. Confirmation of the diagnosis of different forms of MHPA then requires more detailed studies, but dietary treatment of other PKU patients can proceed with confidence.", "contents": "Urinary dihydroxanthopterin in the diagnosis of malignant hyperphenylalaninemia and phenylketonuria. Assessment of urinary dihydroxanthopterin is proposed as a simple method of recognition of patients with malignant hyperphenylalaninemia (MHPA). High levels of urinary dihydroxanthopterin are found in untreated patients with phenylketonuria (PKU) or with dihydropteridine reductase (DHPR) deficiency. After dietary control of the serum phenylalanine level in PKU, the urinary dihydroxanthopterin falls to near normal levels. In DHPR deficiency urinary dihydroxanthopterin levels are high even when serum phenylalanine levels are in the range achieved on dietary treatment. Low levels would be expected in patients with defects in tetrahydrobiopterin synthesis even before dietary treatment. Confirmation of the diagnosis of different forms of MHPA then requires more detailed studies, but dietary treatment of other PKU patients can proceed with confidence."}
{"id": "PMID:487573", "title": "A rapid double antibody non-equilibrium serum thyrotropin radioimmunnoassay suitable for primary neonatal hypothyroidism screening.", "content": "An improved double antibody radioimmunoassay for measuring human thyrotropin (h-TSH) in serum, suitable for neonatal primary hypothyroidism screening is described. The assay employs a non-equilibrium double antibody technique performed at an initial 37 degrees C followed by incubation at room temperature. Studies on the effects of various reaction parameters including time, temperature, antigen and antibody concentrations and the effect of various carrier proteins on the performance of standard curves were also performed. Using a 100-microliter serum sample for analysis, the assay sensitivity is routinely between 3 and 6 microU/mol of h-TSH (MRC 68/38) with interassay and intra-assay coefficient of variation values ranging from 6 to 14% over a wide range of control values. The incubation and phase separation is completed after 26 h with data available following automated counting and computation. Such a rapid assay facilitates a neonatal screening program for the early detection and treatment of primary neonatal hypothyroidism using either cord blood serum or early follow-up serum sampling in infants suspected on initial screening tests of an abnormality.", "contents": "A rapid double antibody non-equilibrium serum thyrotropin radioimmunnoassay suitable for primary neonatal hypothyroidism screening. An improved double antibody radioimmunoassay for measuring human thyrotropin (h-TSH) in serum, suitable for neonatal primary hypothyroidism screening is described. The assay employs a non-equilibrium double antibody technique performed at an initial 37 degrees C followed by incubation at room temperature. Studies on the effects of various reaction parameters including time, temperature, antigen and antibody concentrations and the effect of various carrier proteins on the performance of standard curves were also performed. Using a 100-microliter serum sample for analysis, the assay sensitivity is routinely between 3 and 6 microU/mol of h-TSH (MRC 68/38) with interassay and intra-assay coefficient of variation values ranging from 6 to 14% over a wide range of control values. The incubation and phase separation is completed after 26 h with data available following automated counting and computation. Such a rapid assay facilitates a neonatal screening program for the early detection and treatment of primary neonatal hypothyroidism using either cord blood serum or early follow-up serum sampling in infants suspected on initial screening tests of an abnormality."}
{"id": "PMID:487574", "title": "A simple specific method for the determination of the hemoglobin content of tissue homogenates.", "content": "A simple, inexpensive and specific method for the determination of hemoglobin in tissue homogenates is described. It is based on the specific binding of hemoglobin by haptoglobin and the stabilizing effect of this binding on the peroxidase activity of hemoglobin. The haptoglobin is added as serum and no purification is needed. The method gives reliable estimates of hemoglobin contents above 10 mg/l.", "contents": "A simple specific method for the determination of the hemoglobin content of tissue homogenates. A simple, inexpensive and specific method for the determination of hemoglobin in tissue homogenates is described. It is based on the specific binding of hemoglobin by haptoglobin and the stabilizing effect of this binding on the peroxidase activity of hemoglobin. The haptoglobin is added as serum and no purification is needed. The method gives reliable estimates of hemoglobin contents above 10 mg/l."}
{"id": "PMID:487575", "title": "An improved method for the differential assay of 3-O-methylated catecholamines in human urine using ion-pair extraction and gas chromatography electron capture detection.", "content": "A gas chromatographic method is described for the quantitative determination of urinary normetadrenaline, metadrenaline and 3-methoxytyramine using electron capture detection. The N,O-dipentafluoropropionyl beta-O-ethyl derivatives of normetadrenaline and metadrenaline and the N,O-dipentafluoropropionyl derivative of 3-methoxytyramine were prepared. The amines were purified by ion exchange chromatography and ion pair extraction. Amine derivatives from urine extracts were identified definitively by mass fragmentography. The 24-h excretion value for normetadrenaline in human urine is 167 +/- 95 micrograms (mean +/- S.D.), for metadrenaline is 116 +/- 74 micrograms (mean +/- S.D.) and for 3-methoxytyramine is 82 +/- 68 micrograms (mean +/- S.D.).", "contents": "An improved method for the differential assay of 3-O-methylated catecholamines in human urine using ion-pair extraction and gas chromatography electron capture detection. A gas chromatographic method is described for the quantitative determination of urinary normetadrenaline, metadrenaline and 3-methoxytyramine using electron capture detection. The N,O-dipentafluoropropionyl beta-O-ethyl derivatives of normetadrenaline and metadrenaline and the N,O-dipentafluoropropionyl derivative of 3-methoxytyramine were prepared. The amines were purified by ion exchange chromatography and ion pair extraction. Amine derivatives from urine extracts were identified definitively by mass fragmentography. The 24-h excretion value for normetadrenaline in human urine is 167 +/- 95 micrograms (mean +/- S.D.), for metadrenaline is 116 +/- 74 micrograms (mean +/- S.D.) and for 3-methoxytyramine is 82 +/- 68 micrograms (mean +/- S.D.)."}
{"id": "PMID:487576", "title": "A new colorimetric method for the determination of serum enzyme, gamma-glutamyl transpeptidase, cystine aminopeptidase, and leucine aminopeptidase.", "content": "A new colorimetric method for the serum enzyme assay was developed, using the color reaction of a ferrous salt complex and a primary aromatic amine. For this method new substrates containing primary aromatic amines were synthesized. The aromatic amine liberated by the enzyme reaction was measured by its color reaction with pentacyanoammineferroate treated with hydrogen peroxide. The determination was made at a wavelength of around 700 nm, at which the assay is not affected by colored substances in serum. Therefore a blank assay was not necessary. This method was applied to the assay of serum gamma-glutamyl transpeptidase, cystine aminopeptidase, and leucine aminopeptidase. The results obtained were in good agreement with those of the p-nitroanilide method also used to assay each enzyme. This new method can be used on a Technicon autoanalyzer system.", "contents": "A new colorimetric method for the determination of serum enzyme, gamma-glutamyl transpeptidase, cystine aminopeptidase, and leucine aminopeptidase. A new colorimetric method for the serum enzyme assay was developed, using the color reaction of a ferrous salt complex and a primary aromatic amine. For this method new substrates containing primary aromatic amines were synthesized. The aromatic amine liberated by the enzyme reaction was measured by its color reaction with pentacyanoammineferroate treated with hydrogen peroxide. The determination was made at a wavelength of around 700 nm, at which the assay is not affected by colored substances in serum. Therefore a blank assay was not necessary. This method was applied to the assay of serum gamma-glutamyl transpeptidase, cystine aminopeptidase, and leucine aminopeptidase. The results obtained were in good agreement with those of the p-nitroanilide method also used to assay each enzyme. This new method can be used on a Technicon autoanalyzer system."}
{"id": "PMID:487577", "title": "A rapid ultraviolet spectrophotometric procedure for the microdetermination of theophylline (1,3-dimethylxanthine) in plasma or serum.", "content": "An ultraviolet spectrophotometric procedure for the microdetermination of theophylline in plasma is described. Theophylline is extracted at pH 5 with chloroform. An aliquot of the solvent is shaken with molar hydrochloric acid and the absorbance of the acid layer determined at 267 nm. The resulting procedure is rapid, reliable, sensitive and virtually specific. It requires 250 microliters sample for a single estimation and has a detection threshold of less than 5 mumol/l.", "contents": "A rapid ultraviolet spectrophotometric procedure for the microdetermination of theophylline (1,3-dimethylxanthine) in plasma or serum. An ultraviolet spectrophotometric procedure for the microdetermination of theophylline in plasma is described. Theophylline is extracted at pH 5 with chloroform. An aliquot of the solvent is shaken with molar hydrochloric acid and the absorbance of the acid layer determined at 267 nm. The resulting procedure is rapid, reliable, sensitive and virtually specific. It requires 250 microliters sample for a single estimation and has a detection threshold of less than 5 mumol/l."}
{"id": "PMID:487578", "title": "Plasma spermine concentrations of patients with benign and malignant tumours of the breast or prostate.", "content": "A radioimmunoassay has been developed for the measurement of plasma spermine concentrations. The sensitivity of the method is 1 pmol spermine/100 microliters plasma and the crossreactivity was 12% with spermidine and 0.18% with putrescine. Plasma spermine levels of patients with benign and malignant tumours of the prostate or breast were measured using this technique. Concentrations were only occasionally elevated in patients with prostatic tumours compared to normal individuals and there was no difference between those men with benign (mean concn. 0.21 +/- 0.14 nmol/ml plasma) or malignant (mean concn. 0.21 +/- 0.11 nmol/ml plasma) tumours. Only 17% of the patients with breast carcinoma had elevated levels of spermine, although there was a significant difference in the concentrations of the breast cancer group of patients compared to normals. No correlation was found between elevated plasma spermine concentrations and tumour grade or presence or spread of metastases in those patients.", "contents": "Plasma spermine concentrations of patients with benign and malignant tumours of the breast or prostate. A radioimmunoassay has been developed for the measurement of plasma spermine concentrations. The sensitivity of the method is 1 pmol spermine/100 microliters plasma and the crossreactivity was 12% with spermidine and 0.18% with putrescine. Plasma spermine levels of patients with benign and malignant tumours of the prostate or breast were measured using this technique. Concentrations were only occasionally elevated in patients with prostatic tumours compared to normal individuals and there was no difference between those men with benign (mean concn. 0.21 +/- 0.14 nmol/ml plasma) or malignant (mean concn. 0.21 +/- 0.11 nmol/ml plasma) tumours. Only 17% of the patients with breast carcinoma had elevated levels of spermine, although there was a significant difference in the concentrations of the breast cancer group of patients compared to normals. No correlation was found between elevated plasma spermine concentrations and tumour grade or presence or spread of metastases in those patients."}
{"id": "PMID:487579", "title": "Plasma lipid analysis by thin-layer chromatography with flame ionisation detection and quantitation.", "content": "A method is presented whereby cholesterol, cholesterol ester, triacylglycerol, non-esterified fatty acid and polar lipid in plasma may be simultaneously quantitated. Following the addition of umbelliferyl palmitate as internal standard, plasma (0.1 ml) is extracted with chloroform/methanol (2 : 1, v/v). A portion of the extract is chromatographed on silica gel fused to a narrow cylindrical quartz rod. After chromatography the rod is traversed through a flame ionisation detector and the responses recorded and integrated. The procedure is shown to be sensitive, linear, reproducible and to compare well with conventional methods for the determination of plasma lipid classes.", "contents": "Plasma lipid analysis by thin-layer chromatography with flame ionisation detection and quantitation. A method is presented whereby cholesterol, cholesterol ester, triacylglycerol, non-esterified fatty acid and polar lipid in plasma may be simultaneously quantitated. Following the addition of umbelliferyl palmitate as internal standard, plasma (0.1 ml) is extracted with chloroform/methanol (2 : 1, v/v). A portion of the extract is chromatographed on silica gel fused to a narrow cylindrical quartz rod. After chromatography the rod is traversed through a flame ionisation detector and the responses recorded and integrated. The procedure is shown to be sensitive, linear, reproducible and to compare well with conventional methods for the determination of plasma lipid classes."}
{"id": "PMID:487580", "title": "The activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase in human cerebrospinal fluid.", "content": "A method for the determination of cyclic nucleotide phosphatase (CNP) activity in cerebrospinal fluid is presented. In normal CSF the activity of CNP is very low. Comparing CSF from patients with multiple sclerosis to control CSF no significant difference is found, although a small proportion of MS patients has an elevated has an elevated CNP activity in their CSF. It is postulated that similar to other substances the CNP activity in CSF probably does not derive from the central nervous system.", "contents": "The activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase in human cerebrospinal fluid. A method for the determination of cyclic nucleotide phosphatase (CNP) activity in cerebrospinal fluid is presented. In normal CSF the activity of CNP is very low. Comparing CSF from patients with multiple sclerosis to control CSF no significant difference is found, although a small proportion of MS patients has an elevated has an elevated CNP activity in their CSF. It is postulated that similar to other substances the CNP activity in CSF probably does not derive from the central nervous system."}
{"id": "PMID:487582", "title": "The production of antibodies to vitamin B-12.", "content": "The conjugation of vitamin B-12 to HSA by means of a combined ethylcarbodiimide N-hydroxysuccinimide conjugation method at pH 5.5 is described. It gave a ratio of 2.7 mol vitamin B-12/mol HSA. Five rabbits were immunized with this conjugate emulsified in Freund's complete adjuvant. After one booster injection high titre antisera (1 : 450 000--1 : 1 500 00) to vitamin B-12 were collected.", "contents": "The production of antibodies to vitamin B-12. The conjugation of vitamin B-12 to HSA by means of a combined ethylcarbodiimide N-hydroxysuccinimide conjugation method at pH 5.5 is described. It gave a ratio of 2.7 mol vitamin B-12/mol HSA. Five rabbits were immunized with this conjugate emulsified in Freund's complete adjuvant. After one booster injection high titre antisera (1 : 450 000--1 : 1 500 00) to vitamin B-12 were collected."}
{"id": "PMID:487583", "title": "The application of a simple, sensitive disc assay for sialyltransferase activity to particulate and soluble enzyme preparations and its use in measuring enzymatic activity in human plasma samples.", "content": "A simple assay for the determination of sialyltransferase activity is described. The method involves the isolation of the radioactive reaction product on cellulose paper discs washed with trichloroacetic acid, thereby greatly facilitating the handling of large numbers of assays. This procedure is both more accurate and more sensitive than that involving precipitation with protein denaturants and separation by centrifugation, and is applicable to both soluble and particulate enzyme preparations. The application of the method to the determination of sialytransferase in human plasma is detailed. Enzyme activity is elevated two-fold in both cancer patients and patients with non-malignant disease. This suggests that the diagnostic use of determinations of sialyltransferase activity may be limited by its non-specificity.", "contents": "The application of a simple, sensitive disc assay for sialyltransferase activity to particulate and soluble enzyme preparations and its use in measuring enzymatic activity in human plasma samples. A simple assay for the determination of sialyltransferase activity is described. The method involves the isolation of the radioactive reaction product on cellulose paper discs washed with trichloroacetic acid, thereby greatly facilitating the handling of large numbers of assays. This procedure is both more accurate and more sensitive than that involving precipitation with protein denaturants and separation by centrifugation, and is applicable to both soluble and particulate enzyme preparations. The application of the method to the determination of sialytransferase in human plasma is detailed. Enzyme activity is elevated two-fold in both cancer patients and patients with non-malignant disease. This suggests that the diagnostic use of determinations of sialyltransferase activity may be limited by its non-specificity."}
{"id": "PMID:487584", "title": "Isolation and characterization of a polyamine-peptide conjugate from human amniotic fluid.", "content": "Significant amounts of the diamine putrescine and the polyamines spermidine and spermine could be detected in human third-trimester amniotic fluid only after acid hydrolysis. This observation was interpreted to mean that these amines existed only in conjugated form in this biological fluid. Upon fractionation by ultrafiltration 90--10% of the putrescine was associated with the 1000--10 000 dalton fraction. Spermine was identified in this fraction and in a low-molecular weight fraction presumably representing acetylated derivatives. Spermidine was entirely associated with the 10 000--30 000 dalton fraction. The putrescine conjugate was purified to homogeneity by column chromatography on Biogels P10 and P6 followed by ion-exchange chromatography on DEAE-Sephadex A-25. Molecular weight by gel exclusion using peptide standards was estimated to be approx. 4600. The UV absorption spectrum of the putrescine conjugate conformed to that expected for a polypeptide. This putrescine conjugate contained 39 identified amino acids with a combined molecular weight of 4713. Putrescine was detectable by high pressure liquid chromatography only after acid hydrolysis of the conjugate. No other polyamines were detected in these hydrolyzates, nor were any polyamines demonstable in hydrolyzates of control peptides nor in pooled column washes. The identity of the putrescine determined by high pressure liquid chromatography was confirmed by a two-dimensional thin-layer chromatography method. These results establish the in vivo production of a putrescine--polypeptide conjugate in man. Such molecular species may constitute yet another metabolic pathway for polyamines or may reflect another mode of post-translational modification of polypeptide structure and function. The qualitative and quantitative analysis of polyamine conjugate in human aminotic fluid may prove to be useful in the detection of abnormalities in fetal development.", "contents": "Isolation and characterization of a polyamine-peptide conjugate from human amniotic fluid. Significant amounts of the diamine putrescine and the polyamines spermidine and spermine could be detected in human third-trimester amniotic fluid only after acid hydrolysis. This observation was interpreted to mean that these amines existed only in conjugated form in this biological fluid. Upon fractionation by ultrafiltration 90--10% of the putrescine was associated with the 1000--10 000 dalton fraction. Spermine was identified in this fraction and in a low-molecular weight fraction presumably representing acetylated derivatives. Spermidine was entirely associated with the 10 000--30 000 dalton fraction. The putrescine conjugate was purified to homogeneity by column chromatography on Biogels P10 and P6 followed by ion-exchange chromatography on DEAE-Sephadex A-25. Molecular weight by gel exclusion using peptide standards was estimated to be approx. 4600. The UV absorption spectrum of the putrescine conjugate conformed to that expected for a polypeptide. This putrescine conjugate contained 39 identified amino acids with a combined molecular weight of 4713. Putrescine was detectable by high pressure liquid chromatography only after acid hydrolysis of the conjugate. No other polyamines were detected in these hydrolyzates, nor were any polyamines demonstable in hydrolyzates of control peptides nor in pooled column washes. The identity of the putrescine determined by high pressure liquid chromatography was confirmed by a two-dimensional thin-layer chromatography method. These results establish the in vivo production of a putrescine--polypeptide conjugate in man. Such molecular species may constitute yet another metabolic pathway for polyamines or may reflect another mode of post-translational modification of polypeptide structure and function. The qualitative and quantitative analysis of polyamine conjugate in human aminotic fluid may prove to be useful in the detection of abnormalities in fetal development."}
{"id": "PMID:487585", "title": "Automated radioimmunoassay of nicotine.", "content": "We have developed an automated nonequilibrium procedure for the radioimmunoassay of nicotine. The use of a unique iodinated nicotine derivative in this procedure gave a sensitivity of 10 micrograms/l for nicotine with a between-run precision of 7.4% and within-run precision of 6.0%. Nicotine levels of 60 to 67 micrograms/ml were found in subjects 15 min after smoking one standard cigarette. The technique herein reported is a very rapid, and sensitive radioimmunoassay for nicotine and facilitates the determination of nicotine in smoking subjects during the actual process of smoking.", "contents": "Automated radioimmunoassay of nicotine. We have developed an automated nonequilibrium procedure for the radioimmunoassay of nicotine. The use of a unique iodinated nicotine derivative in this procedure gave a sensitivity of 10 micrograms/l for nicotine with a between-run precision of 7.4% and within-run precision of 6.0%. Nicotine levels of 60 to 67 micrograms/ml were found in subjects 15 min after smoking one standard cigarette. The technique herein reported is a very rapid, and sensitive radioimmunoassay for nicotine and facilitates the determination of nicotine in smoking subjects during the actual process of smoking."}
{"id": "PMID:487586", "title": "The clinical measurement of urinary total hydroxyproline excretion.", "content": "The effect of dietary control on the day-to-day variation in total hydroxyproline excretion has been examined in two studies. In the first, a normal volunteer ate a controlled diet containing varying gelatin supplements for several weeks. In the second, the effect of removing hydroxyproline-containing foods from the diets of 8 volunteers was examined. Both studies confirm that the day-to-day variation in total hydroxyproline excretion falls when the gelatin content of the diet is decreased, whether the results are expressed as total hydroxyproline excretion rate or as the total hydroxyproline: creatinine ratio. This fall in variation takes place within 24 h of dietary control beginning and therefore longer periods of dietary restrictions to achieve optimum precision in the measurement of total hydroxyproline are unnecessary. For some analytical methods, results are better expressed as the ratio total hydroxyproline:creatinine than as the total hydroxyproline excretion rate.", "contents": "The clinical measurement of urinary total hydroxyproline excretion. The effect of dietary control on the day-to-day variation in total hydroxyproline excretion has been examined in two studies. In the first, a normal volunteer ate a controlled diet containing varying gelatin supplements for several weeks. In the second, the effect of removing hydroxyproline-containing foods from the diets of 8 volunteers was examined. Both studies confirm that the day-to-day variation in total hydroxyproline excretion falls when the gelatin content of the diet is decreased, whether the results are expressed as total hydroxyproline excretion rate or as the total hydroxyproline: creatinine ratio. This fall in variation takes place within 24 h of dietary control beginning and therefore longer periods of dietary restrictions to achieve optimum precision in the measurement of total hydroxyproline are unnecessary. For some analytical methods, results are better expressed as the ratio total hydroxyproline:creatinine than as the total hydroxyproline excretion rate."}
{"id": "PMID:487587", "title": "Dynamics and composition of serum lipids in hyperlipoproteinemias.", "content": "The microviscosity of serum lipids in patients with hyperlipoproteinemia and normal donors was determined by monitoring the degree of fluorescence polarization of the fluorescent probe 1,6-diphenyl-1,3,5-hexatrine (DPH). Determination of serum lipids--serum total cholesterol, serum triglycerides, and serum phospholipids--revealed that an increase in the ratio of cholesterol/triglyceride + phospholipid is accompanied by an increase in the degree of fluorescence polarization and an increase in the microviscosity values. Similar results were obtained with sonicated liposomes prepared from serum lipids extracts. This correlation between dynamics and composition of serum lipids was further supported by results obtained with an artificial model system of sonicated lipids dispersions. The results have shown that high microviscosity values are characteristic in patients with high levels of serum cholesterol, and that low microviscosity values are characteristic in patients with high levels of serum triglycerides. It is suggested therefore that this technique may serve as a basis for a rapid screening test for hyperlipoproteinemias.", "contents": "Dynamics and composition of serum lipids in hyperlipoproteinemias. The microviscosity of serum lipids in patients with hyperlipoproteinemia and normal donors was determined by monitoring the degree of fluorescence polarization of the fluorescent probe 1,6-diphenyl-1,3,5-hexatrine (DPH). Determination of serum lipids--serum total cholesterol, serum triglycerides, and serum phospholipids--revealed that an increase in the ratio of cholesterol/triglyceride + phospholipid is accompanied by an increase in the degree of fluorescence polarization and an increase in the microviscosity values. Similar results were obtained with sonicated liposomes prepared from serum lipids extracts. This correlation between dynamics and composition of serum lipids was further supported by results obtained with an artificial model system of sonicated lipids dispersions. The results have shown that high microviscosity values are characteristic in patients with high levels of serum cholesterol, and that low microviscosity values are characteristic in patients with high levels of serum triglycerides. It is suggested therefore that this technique may serve as a basis for a rapid screening test for hyperlipoproteinemias."}
{"id": "PMID:487588", "title": "An improved gas-chromatographic method for the determination of sulfated and unsulfated bile acids in serum.", "content": "This study has been aimed at improving some steps in the gas-liquid chromatographic determination of sulfated bile acids. The best conditions for the enzymatic hydrolysis with cholylglycine hydrolase of sulfolithocholylglycine and sulfolithocholyltaurine are described. Recoveries of more than 85% were obtained after prolonging the incubation to 12 h. A single-step procedure for solvolysis and methylation of bile acids was achieved by using, as a water scavenger, 2,2-dimethoxypropane added directly to the hydrolysis mixture. This procedure avoided the difficulty of the extraction of sulfated bile acids from aqueous solutions.", "contents": "An improved gas-chromatographic method for the determination of sulfated and unsulfated bile acids in serum. This study has been aimed at improving some steps in the gas-liquid chromatographic determination of sulfated bile acids. The best conditions for the enzymatic hydrolysis with cholylglycine hydrolase of sulfolithocholylglycine and sulfolithocholyltaurine are described. Recoveries of more than 85% were obtained after prolonging the incubation to 12 h. A single-step procedure for solvolysis and methylation of bile acids was achieved by using, as a water scavenger, 2,2-dimethoxypropane added directly to the hydrolysis mixture. This procedure avoided the difficulty of the extraction of sulfated bile acids from aqueous solutions."}
{"id": "PMID:487589", "title": "The radioimmunoassay of placental protein 5 and circulating levels in maternal blood in the third trimester of normal pregnancy.", "content": "A radioimmunoassay has been developed for placental protein 5 (PP5), a product of the human placenta. Circulating concentrations of PP5 were measured in the third trimester of normal pregnancy in 400 women. Concentrations of PP5 showed a skewed distribution and rose progressively to reach a plateau in the last four weeks of pregnancy. The development of this assay will permit studies on the potential clinical application of maternal PP5 levels during normal and abnormal pregnancy.", "contents": "The radioimmunoassay of placental protein 5 and circulating levels in maternal blood in the third trimester of normal pregnancy. A radioimmunoassay has been developed for placental protein 5 (PP5), a product of the human placenta. Circulating concentrations of PP5 were measured in the third trimester of normal pregnancy in 400 women. Concentrations of PP5 showed a skewed distribution and rose progressively to reach a plateau in the last four weeks of pregnancy. The development of this assay will permit studies on the potential clinical application of maternal PP5 levels during normal and abnormal pregnancy."}
{"id": "PMID:487590", "title": "The urinary excretion of 3-methoxy-4-hydroxyphenylglyc\u00f3l in acute myocardial infarction.", "content": "Urinary excretion of 3-methoxy-4-hydroxyphenylglycol (MHPG), noradrenaline, adrenaline and vanillylmandelic acid (VMA) was studied in 30 patients with acute myocardial infarction. The excretion of MHPG was higher in patients with myocardial infarction than in the control group, with no difference between uncomplicated and complicated courses of the disease. The excretion of MHPG showed a negative although not significant correlation with noradrenaline and VMA during the first days of infarction and a statistically significant positive correlation on the 5th day of the disease. The possible causes of the changes observed are discussed.", "contents": "The urinary excretion of 3-methoxy-4-hydroxyphenylglyc\u00f3l in acute myocardial infarction. Urinary excretion of 3-methoxy-4-hydroxyphenylglycol (MHPG), noradrenaline, adrenaline and vanillylmandelic acid (VMA) was studied in 30 patients with acute myocardial infarction. The excretion of MHPG was higher in patients with myocardial infarction than in the control group, with no difference between uncomplicated and complicated courses of the disease. The excretion of MHPG showed a negative although not significant correlation with noradrenaline and VMA during the first days of infarction and a statistically significant positive correlation on the 5th day of the disease. The possible causes of the changes observed are discussed."}
{"id": "PMID:487592", "title": "Determination of noradrenaline and adrenaline in plasma by a radioenzymatic assay using high pressure liquid chromatography for the separation of the radiochemical products.", "content": "Plasma catecholamines are determined by a radioenzymatic assay using high pressure liquid chromatography for the separation of the labelled radiochemical products. Noradrenaline and adrenaline are converted to their O-methylated analogues, normethanephrine and metanephrine, by the enzyme catechol O-methyltransferase in the presence of tritiated S-adenosyl-L-methionine. Non-radioactive carriers are added, whereupon the metanephrines are extracted in an organic phase and re-extracted in an aqueous phase. The aqueous phase is dried, the residue taken up in a phosphate solution and chromatographed. The normetanephrine and metanephrine peaks are collected, converted to vanillin and assayed for radioactivity. The detection limit for noradrenaline is 32 ng/l plasma and for adrenaline 16 ng/l plasma. Intra- and interassay variables are respectively 6.2 and 10% for noradrenaline and 5.3 and 8.8% for adrenaline. The mean supine levels of noradrenaline and adrenaline in normal subjects are 355 and 61 ng/l plasma, respectively. This highly specific procedure takes two days for one person to assay 45 blood samples and can be reduced to one day if the chromatography step is automated.", "contents": "Determination of noradrenaline and adrenaline in plasma by a radioenzymatic assay using high pressure liquid chromatography for the separation of the radiochemical products. Plasma catecholamines are determined by a radioenzymatic assay using high pressure liquid chromatography for the separation of the labelled radiochemical products. Noradrenaline and adrenaline are converted to their O-methylated analogues, normethanephrine and metanephrine, by the enzyme catechol O-methyltransferase in the presence of tritiated S-adenosyl-L-methionine. Non-radioactive carriers are added, whereupon the metanephrines are extracted in an organic phase and re-extracted in an aqueous phase. The aqueous phase is dried, the residue taken up in a phosphate solution and chromatographed. The normetanephrine and metanephrine peaks are collected, converted to vanillin and assayed for radioactivity. The detection limit for noradrenaline is 32 ng/l plasma and for adrenaline 16 ng/l plasma. Intra- and interassay variables are respectively 6.2 and 10% for noradrenaline and 5.3 and 8.8% for adrenaline. The mean supine levels of noradrenaline and adrenaline in normal subjects are 355 and 61 ng/l plasma, respectively. This highly specific procedure takes two days for one person to assay 45 blood samples and can be reduced to one day if the chromatography step is automated."}
{"id": "PMID:487593", "title": "Plasma somatomedin activity in vitamin A deficient children.", "content": "A role for vitamin A in the sulphation of mucopolysaccharides has been suggested. Somatomedin, a growth hormone dependent serum factor, has also been shown to stimulate the uptake of sulphate by cartilage. Therefore studies were undertaken on vitamin A deficient children to examine the possible interrelationship between vitamin A and somatomedin activity. Plasma somatomedin activity was markedly lowered in vitamin A deficient children and plasma HGH levels were in the normal range. The data suggest that vitamin A and somatomedin may be interrelated and also that plasma somatomedin activity may not always be determined by plasma growth hormone levels.", "contents": "Plasma somatomedin activity in vitamin A deficient children. A role for vitamin A in the sulphation of mucopolysaccharides has been suggested. Somatomedin, a growth hormone dependent serum factor, has also been shown to stimulate the uptake of sulphate by cartilage. Therefore studies were undertaken on vitamin A deficient children to examine the possible interrelationship between vitamin A and somatomedin activity. Plasma somatomedin activity was markedly lowered in vitamin A deficient children and plasma HGH levels were in the normal range. The data suggest that vitamin A and somatomedin may be interrelated and also that plasma somatomedin activity may not always be determined by plasma growth hormone levels."}
{"id": "PMID:487594", "title": "A gas chromatographic-mass spectrometric assay for nine hydroxyphenolic acids in uremic serum.", "content": "A mass fragmentographic method for the determination of m-hydroxybenzoic acid, p-hydroxybenzoic acid, o-hydroxyphenylacetic acid, m-hydroxyphenylacetic acid, p-hydroxyphenylacetic acid, vanillic acid, p-hydroxymandelic acid, homovanillic acid and vanilmandelic acid in uremic serum is described. The hydroxyphenolic acids were extracted from one ml of serum ultrafiltrate. After trimethylsilylation, the extract was analyzed by a gas chromatograph-mass spectrometer. Concentrations of nine hydroxyphenolic acids are higher in uremic serum. In particular, p-hydroxybenzoic acid and p-hydroxyphenylacetic acid are markedly increased in uremic serum. The influence of hemodialysis on the hydroxyphenolic acids was studied by quantitating the acids before and after hemodialysis.", "contents": "A gas chromatographic-mass spectrometric assay for nine hydroxyphenolic acids in uremic serum. A mass fragmentographic method for the determination of m-hydroxybenzoic acid, p-hydroxybenzoic acid, o-hydroxyphenylacetic acid, m-hydroxyphenylacetic acid, p-hydroxyphenylacetic acid, vanillic acid, p-hydroxymandelic acid, homovanillic acid and vanilmandelic acid in uremic serum is described. The hydroxyphenolic acids were extracted from one ml of serum ultrafiltrate. After trimethylsilylation, the extract was analyzed by a gas chromatograph-mass spectrometer. Concentrations of nine hydroxyphenolic acids are higher in uremic serum. In particular, p-hydroxybenzoic acid and p-hydroxyphenylacetic acid are markedly increased in uremic serum. The influence of hemodialysis on the hydroxyphenolic acids was studied by quantitating the acids before and after hemodialysis."}
{"id": "PMID:487595", "title": "Turbidimetric determination of lipoprotein-X in serum.", "content": "Turbidimetric determination of the cholestasis characterizing lipoprotein-X is based on the separation from the serum investigated of interfering lipoproteins by precipitation with the appropriate concentration of heparin and zinc acetate followed by centrifugation. In the supernatant the abnormal lipoprotein-X, which is not precipitated under these conditions, was determined by measuring the turbidity after addition of calcium chloride and heparin.", "contents": "Turbidimetric determination of lipoprotein-X in serum. Turbidimetric determination of the cholestasis characterizing lipoprotein-X is based on the separation from the serum investigated of interfering lipoproteins by precipitation with the appropriate concentration of heparin and zinc acetate followed by centrifugation. In the supernatant the abnormal lipoprotein-X, which is not precipitated under these conditions, was determined by measuring the turbidity after addition of calcium chloride and heparin."}
{"id": "PMID:487598", "title": "Derivative electrophoretograms and their applications.", "content": "A differentiator was constructed to produce the first and second derivative curves of routine cellulose acetate membrane electrophoretograms of human serum protein. The derivative curves could reveal the presence of multicomponent in each globulin fraction. The \"M\" components could be detected easily and semiquantitatively, when the cellulose acetate membrane electrophoretic pattern was evaluated by the derivative curves. Utilization of the derivative curves could be a useful adjunct to the detection of an \"M\" component since the presence of a narrow band produces a sharper peak on the second derivative curve and more indentation on the first derivative curve.", "contents": "Derivative electrophoretograms and their applications. A differentiator was constructed to produce the first and second derivative curves of routine cellulose acetate membrane electrophoretograms of human serum protein. The derivative curves could reveal the presence of multicomponent in each globulin fraction. The \"M\" components could be detected easily and semiquantitatively, when the cellulose acetate membrane electrophoretic pattern was evaluated by the derivative curves. Utilization of the derivative curves could be a useful adjunct to the detection of an \"M\" component since the presence of a narrow band produces a sharper peak on the second derivative curve and more indentation on the first derivative curve."}
{"id": "PMID:487599", "title": "A simple method of lysosomal hydrolase assay in a single somatic cell and its application.", "content": "A new and simple assay method for lysosomal hydrolase in a single cultured skin fibroblast is described. Using 4-methylumbelliferyl-glycosides and ordinary laboratory equipment, the activity can be measured with the highest sensitivity of 0.5 pmol of 4-methylumbelliferone released from the substrates. Two systems were compared and the methods described proved reliable for the diagnosis of several variants of beta-galactosidase deficiency.", "contents": "A simple method of lysosomal hydrolase assay in a single somatic cell and its application. A new and simple assay method for lysosomal hydrolase in a single cultured skin fibroblast is described. Using 4-methylumbelliferyl-glycosides and ordinary laboratory equipment, the activity can be measured with the highest sensitivity of 0.5 pmol of 4-methylumbelliferone released from the substrates. Two systems were compared and the methods described proved reliable for the diagnosis of several variants of beta-galactosidase deficiency."}
{"id": "PMID:487600", "title": "An ultrasensitive method for the measurement of human leukocyte calcium: lymphocytes.", "content": "Studies of the transport and distribution of calcium in leukocytes have been severely hampered by the inability to measure accurately and reproducibly the concentration of calcium in small numbers of cells. We have applied a recent development in analytical chemistry, the graphite furnace atomic absorption spectrophotometer, to this problem. The calcium content of human blood lymphocytes was determined by both graphite furnace and conventional flame atomic absorption spectrophotometry. The linearity, sensitivity and detection limits of the two techniques were compared. For measurement of calcium, the graphite furnace sensitivity was 55 times higher in aqueous samples and 60 times higher in cell samples than the flame technique. The detection limit of the graphite furnace was 800 times lower in aqueous samples and 1500 times lower in cell samples. The enhanced sensitivity of this technique allowed us to prepare samples with 20 times fewer blood cells. We have employed this graphite furnace technique to measure lymphocyte calcium content and its relationship to the calcium concentration and proportion of serum in the suspending medium. In the absence of serum, the lymphocyte calcium content approximately doubled as the medium calcium concentration was increased from 1 mumol/l to 0.5 mmol/l. At medium calcium concentrations of 0.5 mmol/l and above, the lymphocyte calcium content was 1.0 mmol/l cells. In medium adjusted to 2 mmol/l calcium, the lymphocyte calcium content approximately doubled as the medium serum concentration was increased from 0 to 2%. At medium serum concentrations of 2% and above, lymphocyte calcium content was 2 mmol/l cells. The exchangeable cell calcium, measured with 45Ca in the same samples, did not increase as serum was added to the medium.", "contents": "An ultrasensitive method for the measurement of human leukocyte calcium: lymphocytes. Studies of the transport and distribution of calcium in leukocytes have been severely hampered by the inability to measure accurately and reproducibly the concentration of calcium in small numbers of cells. We have applied a recent development in analytical chemistry, the graphite furnace atomic absorption spectrophotometer, to this problem. The calcium content of human blood lymphocytes was determined by both graphite furnace and conventional flame atomic absorption spectrophotometry. The linearity, sensitivity and detection limits of the two techniques were compared. For measurement of calcium, the graphite furnace sensitivity was 55 times higher in aqueous samples and 60 times higher in cell samples than the flame technique. The detection limit of the graphite furnace was 800 times lower in aqueous samples and 1500 times lower in cell samples. The enhanced sensitivity of this technique allowed us to prepare samples with 20 times fewer blood cells. We have employed this graphite furnace technique to measure lymphocyte calcium content and its relationship to the calcium concentration and proportion of serum in the suspending medium. In the absence of serum, the lymphocyte calcium content approximately doubled as the medium calcium concentration was increased from 1 mumol/l to 0.5 mmol/l. At medium calcium concentrations of 0.5 mmol/l and above, the lymphocyte calcium content was 1.0 mmol/l cells. In medium adjusted to 2 mmol/l calcium, the lymphocyte calcium content approximately doubled as the medium serum concentration was increased from 0 to 2%. At medium serum concentrations of 2% and above, lymphocyte calcium content was 2 mmol/l cells. The exchangeable cell calcium, measured with 45Ca in the same samples, did not increase as serum was added to the medium."}
{"id": "PMID:487601", "title": "Human inosine triphosphatase: catalytic properties and population studies.", "content": "Using a reliable 2-step colorimetric assay, some catalytic properties of human erythrocyte ITPase (inosine triphosphate pyrophosphohydrolase) (EC 3.6.1.19) were determined. The enzyme hydrolyses ITP, dITP (deoxyinosine triphosphate) and XTP (xanthine triphosphate) but not other nucleotides. IDP is a competitive inhibitor. Subcellular fractionation of cultured lymphoid-line cells showed ITPase activity to be in the cytosol. ATP and GTP hydrolysing activities were not enriched in the cytosol fraction. The range of activity was investigated in erythrocytes, peripheral leukocytes, amniotic fluid cells, skin fibroblasts, long term lymphoid lines and bone marrow fibroblasts. The mean ITPase activity was found to vary in different cell types from 4.9 to 294 units per mg protein. Fractionation of red blood cells by age revealed that the enzyme decays exponentially with erythrocyte age with a half-life of 35 days. The enzyme had a wide range of specific activity in erythrocytes from 150 normal randomly selected individuals (15--722 pmol/h/g Hb). Within the low range of erythrocyte specific activities (from 15 to 200) a correlation was found between an individual's erythrocyte activity and the activity in peripheral leukocytes. This correlation was not found at higher erythrocyte ITPase activities. Two individuals were detected who had erythrocyte ITPase activities which were close to the lowest ITPase activities of all samples tested. Thus, low or absent ITPase activity is an individual characteristic which may be expressed both in erythrocytes and in other cell types.", "contents": "Human inosine triphosphatase: catalytic properties and population studies. Using a reliable 2-step colorimetric assay, some catalytic properties of human erythrocyte ITPase (inosine triphosphate pyrophosphohydrolase) (EC 3.6.1.19) were determined. The enzyme hydrolyses ITP, dITP (deoxyinosine triphosphate) and XTP (xanthine triphosphate) but not other nucleotides. IDP is a competitive inhibitor. Subcellular fractionation of cultured lymphoid-line cells showed ITPase activity to be in the cytosol. ATP and GTP hydrolysing activities were not enriched in the cytosol fraction. The range of activity was investigated in erythrocytes, peripheral leukocytes, amniotic fluid cells, skin fibroblasts, long term lymphoid lines and bone marrow fibroblasts. The mean ITPase activity was found to vary in different cell types from 4.9 to 294 units per mg protein. Fractionation of red blood cells by age revealed that the enzyme decays exponentially with erythrocyte age with a half-life of 35 days. The enzyme had a wide range of specific activity in erythrocytes from 150 normal randomly selected individuals (15--722 pmol/h/g Hb). Within the low range of erythrocyte specific activities (from 15 to 200) a correlation was found between an individual's erythrocyte activity and the activity in peripheral leukocytes. This correlation was not found at higher erythrocyte ITPase activities. Two individuals were detected who had erythrocyte ITPase activities which were close to the lowest ITPase activities of all samples tested. Thus, low or absent ITPase activity is an individual characteristic which may be expressed both in erythrocytes and in other cell types."}
{"id": "PMID:487602", "title": "High performance liquid chromatographic determination of serum UV profiles of normal subjects and patients with breast cancer and benign fibrocystic changes.", "content": "High performance liquid chromatography (HPLC) was used to determine the UV profiles of serum samples taken postoperatively from 22 patients with histologically documented breast cancer, 8 patients with benign breast fibrocystic changes and 10 normal subjects. The analyses were performed on coded serum samples and after they were completed, the code was broken and the results correlated with the clinical data. Only one ml of serum was required for the HPLC analysis and identification. Detection limits for the nucleosides and bases were in the 10--20 pmol range and the injection volume of the deproteinated serum was 75 mul. The UV profiles of the normal subjects were very reproducible and similar to those of the patients with benign fibrocystic changes. The profiles of some of the cancer patients were distinctly different from the two other groups, 1-methylinosine and N2-methylguanosine, which were not detected in sera from normal subjects and patients with benign fibrocystic changes, were found in 45.5% and 22.7% of the cancer patients, respectively. Patients with the metastatic disease also showed elevated levels of guanosine and uridine. Only one false positive was found in the normal population. At present, it is not clear whether this indicates a subclinical manifestation of the disease and it must await further follow-up.", "contents": "High performance liquid chromatographic determination of serum UV profiles of normal subjects and patients with breast cancer and benign fibrocystic changes. High performance liquid chromatography (HPLC) was used to determine the UV profiles of serum samples taken postoperatively from 22 patients with histologically documented breast cancer, 8 patients with benign breast fibrocystic changes and 10 normal subjects. The analyses were performed on coded serum samples and after they were completed, the code was broken and the results correlated with the clinical data. Only one ml of serum was required for the HPLC analysis and identification. Detection limits for the nucleosides and bases were in the 10--20 pmol range and the injection volume of the deproteinated serum was 75 mul. The UV profiles of the normal subjects were very reproducible and similar to those of the patients with benign fibrocystic changes. The profiles of some of the cancer patients were distinctly different from the two other groups, 1-methylinosine and N2-methylguanosine, which were not detected in sera from normal subjects and patients with benign fibrocystic changes, were found in 45.5% and 22.7% of the cancer patients, respectively. Patients with the metastatic disease also showed elevated levels of guanosine and uridine. Only one false positive was found in the normal population. At present, it is not clear whether this indicates a subclinical manifestation of the disease and it must await further follow-up."}
{"id": "PMID:487603", "title": "N-Acetyl-beta-D-glucosaminidase levels and diabetic microangiopathy.", "content": "N-Acetyl-beta-D-glucosaminidase (NAG) activity has been measured in the serum and urine of primary and secondary diabetics and in primary diabetics with microangiopathy. NAG activity has also been measured in the tears of diabetics with ocular complications and diabetics with no ocular changes. Results have shown significantly higher levels of urinary NAG in diabetics with proteinuria (p less than 0.001) and proteinuria and retinopathy (p less than 0.001). There was no correlation between urinary NAG activity and serum creatinine (r = 0.28) or urinary NAG and the degree of proteinuria (r = 0.24). Increased urinary NAG levels were also observed in secondary diabetes associated with haemochromatosis and acromegaly. Significantly higher serum NAG levels were found in newly diagnosed diabetics (p less than 0.01) and significantly lower levels in chemical diabetics (p less than 0.01). Compared to non-diabetic controls tear NAG levels were significantly higher in the diabetic controls (p less than 0.01), in diabetics with retinopathy (p less than 0.01), and in diabetics with cataract formation (p less than 0.05). An assessment of this enzyme is made in relation to the development of diabetic microangiopathy.", "contents": "N-Acetyl-beta-D-glucosaminidase levels and diabetic microangiopathy. N-Acetyl-beta-D-glucosaminidase (NAG) activity has been measured in the serum and urine of primary and secondary diabetics and in primary diabetics with microangiopathy. NAG activity has also been measured in the tears of diabetics with ocular complications and diabetics with no ocular changes. Results have shown significantly higher levels of urinary NAG in diabetics with proteinuria (p less than 0.001) and proteinuria and retinopathy (p less than 0.001). There was no correlation between urinary NAG activity and serum creatinine (r = 0.28) or urinary NAG and the degree of proteinuria (r = 0.24). Increased urinary NAG levels were also observed in secondary diabetes associated with haemochromatosis and acromegaly. Significantly higher serum NAG levels were found in newly diagnosed diabetics (p less than 0.01) and significantly lower levels in chemical diabetics (p less than 0.01). Compared to non-diabetic controls tear NAG levels were significantly higher in the diabetic controls (p less than 0.01), in diabetics with retinopathy (p less than 0.01), and in diabetics with cataract formation (p less than 0.05). An assessment of this enzyme is made in relation to the development of diabetic microangiopathy."}
{"id": "PMID:487604", "title": "Plasma trypsin in chronic pancreatitis and pancreatic adenocarcinoma.", "content": "We have used a simple and precise radioimmunoassay to measure trypsin in human plasma. Fasting plasma trypsin concentrations were extremely low in patients with chronic pancreatitis with steatorrhoea (5 +/- 2 ng/ml) when compared to healthy controls (86 +/- 7 ng/ml, p less than 0.001). In patients with chronic pancreatitis but no steatorrhoea basal plasma trypsin levels were similar to those of the normal controls (99 +/- 25 ng/ml). A small but significant postprandial rise in plasma trypsin concentrations was observed in normal subjects (mean increment 15 +/- 4%, p less than 0.005, paired t test) but was absent in patients with chronic pancreatitis with steatorrhoea. In contrast to exocrine deficient chronic pancreatitis, other malabsorptive conditions associated with steatorrhoea (active coeliac disease and acute tropical sprue) demonstrated mean fasting trypsin concentrations similar to controls. Patients with adenocarcinoma of the pancreas had basal trypsin concentrations similar to healthy subjects as did patients with adenocarcinoma of the stomach, colon, rectum, brochus, and breast. In some cases measurement of plasma trypsin may be of help in the differential diagnosis of steatorrhoea.", "contents": "Plasma trypsin in chronic pancreatitis and pancreatic adenocarcinoma. We have used a simple and precise radioimmunoassay to measure trypsin in human plasma. Fasting plasma trypsin concentrations were extremely low in patients with chronic pancreatitis with steatorrhoea (5 +/- 2 ng/ml) when compared to healthy controls (86 +/- 7 ng/ml, p less than 0.001). In patients with chronic pancreatitis but no steatorrhoea basal plasma trypsin levels were similar to those of the normal controls (99 +/- 25 ng/ml). A small but significant postprandial rise in plasma trypsin concentrations was observed in normal subjects (mean increment 15 +/- 4%, p less than 0.005, paired t test) but was absent in patients with chronic pancreatitis with steatorrhoea. In contrast to exocrine deficient chronic pancreatitis, other malabsorptive conditions associated with steatorrhoea (active coeliac disease and acute tropical sprue) demonstrated mean fasting trypsin concentrations similar to controls. Patients with adenocarcinoma of the pancreas had basal trypsin concentrations similar to healthy subjects as did patients with adenocarcinoma of the stomach, colon, rectum, brochus, and breast. In some cases measurement of plasma trypsin may be of help in the differential diagnosis of steatorrhoea."}
{"id": "PMID:487605", "title": "Immunological identity of urinary alkaline phosphatase in health and disease.", "content": "Urinary alkaline phosphatase has been measured and an investigation has been undertaken involving starch gel electrophoresis after treatment of the enzyme with antisera raised against various human tissues. Urine was obtained from patients with bone and liver disease as well as pregnant women and normal persons. The enzyme level in urine in disease and pregnancy was raised above the normal but considerable overlap occurred. After electrophoresis the most usual finding was a single zone of activity although occasionally very minor zones were also present. Antiserum to kidney had the greatest affinity for the urinary enzyme. Decreasing affinity was found with intestinal and placental antiserum. No affinity was found with liver antiserum.", "contents": "Immunological identity of urinary alkaline phosphatase in health and disease. Urinary alkaline phosphatase has been measured and an investigation has been undertaken involving starch gel electrophoresis after treatment of the enzyme with antisera raised against various human tissues. Urine was obtained from patients with bone and liver disease as well as pregnant women and normal persons. The enzyme level in urine in disease and pregnancy was raised above the normal but considerable overlap occurred. After electrophoresis the most usual finding was a single zone of activity although occasionally very minor zones were also present. Antiserum to kidney had the greatest affinity for the urinary enzyme. Decreasing affinity was found with intestinal and placental antiserum. No affinity was found with liver antiserum."}
{"id": "PMID:487606", "title": "Fluorimetric assay of acid lipase in human leukocytes.", "content": "A fluorimetric assay for leukocyte acid lipase, the enzyme defect in Wolman disease, is described. The method requires a smaller volume of blood and can be performed more rapidly than the present colorimetric assay for the leukocyte enzyme.", "contents": "Fluorimetric assay of acid lipase in human leukocytes. A fluorimetric assay for leukocyte acid lipase, the enzyme defect in Wolman disease, is described. The method requires a smaller volume of blood and can be performed more rapidly than the present colorimetric assay for the leukocyte enzyme."}
{"id": "PMID:487607", "title": "Formation of an alkaline phosphatase-immunoglobulin G complex in human sera.", "content": "An electrophoretically slow-moving band of alkaline phosphatase (ALP) isoenzyme was found in four patients with chronic liver disease and in one with ulcerative colitis. Immunoelectrophoretic studies revealed that the slow band was a complex containing alkaline phosphatase and an immunoglobulin G of the lambda class. Its molecular weight was approximately 280 000. A complex molecule consisting of one molecule of immunoglobulin G and one molecule of alkaline phosphatase was proposed. The complex was similar to the liver and bone alkaline phosphatases in functional properties. Serum containing the complex was capable of binding liver and bone alkaline phosphatase isoenzymes but not the intestine or placenta alkaline phosphatase isoenzymes from normal controls. The presence of an abnormal immunoglobulin which binds liver and bone alkaline phosphatases appears to be responsible for the development of the complex. In one case of chronic liver disease, the complex disappeared after a few months.", "contents": "Formation of an alkaline phosphatase-immunoglobulin G complex in human sera. An electrophoretically slow-moving band of alkaline phosphatase (ALP) isoenzyme was found in four patients with chronic liver disease and in one with ulcerative colitis. Immunoelectrophoretic studies revealed that the slow band was a complex containing alkaline phosphatase and an immunoglobulin G of the lambda class. Its molecular weight was approximately 280 000. A complex molecule consisting of one molecule of immunoglobulin G and one molecule of alkaline phosphatase was proposed. The complex was similar to the liver and bone alkaline phosphatases in functional properties. Serum containing the complex was capable of binding liver and bone alkaline phosphatase isoenzymes but not the intestine or placenta alkaline phosphatase isoenzymes from normal controls. The presence of an abnormal immunoglobulin which binds liver and bone alkaline phosphatases appears to be responsible for the development of the complex. In one case of chronic liver disease, the complex disappeared after a few months."}
{"id": "PMID:487608", "title": "Radioimmunoassay for human pancreatic amylase: comparison of human serum amylase by measurement of enzymatic activity and by radioimmunoassay.", "content": "A radioimmunoassay (RIA) for human pancreatic amylase has been developed for the determination of human serum amylase content. The assay was shown to be sensitive (7 ng/ml), reproducible and specific, but human pancreatic amylase and salivary amylase could not be distinguished by the antiserum used. In normal subjects, the mean concentration of amylase determined by the RIA was found to be 122.1 ng/ml (range: 55--250 ng/ml). A good correlation was observed between the concentration of amylase and its enzymatic activity in normal subjects. In some instances with high amylase activity, however, the rise in enzymatic activity was not accompanied by increasing amount of amylase content.", "contents": "Radioimmunoassay for human pancreatic amylase: comparison of human serum amylase by measurement of enzymatic activity and by radioimmunoassay. A radioimmunoassay (RIA) for human pancreatic amylase has been developed for the determination of human serum amylase content. The assay was shown to be sensitive (7 ng/ml), reproducible and specific, but human pancreatic amylase and salivary amylase could not be distinguished by the antiserum used. In normal subjects, the mean concentration of amylase determined by the RIA was found to be 122.1 ng/ml (range: 55--250 ng/ml). A good correlation was observed between the concentration of amylase and its enzymatic activity in normal subjects. In some instances with high amylase activity, however, the rise in enzymatic activity was not accompanied by increasing amount of amylase content."}
{"id": "PMID:487609", "title": "Porphobilinogen oxygenase from human erythrocytes.", "content": "Porphobilinogen oxygenase was isolated from red cells of healthy persons and of patients with disturbances in porphyrin metabolism. The hemolysates were purified by DEAE-cellulose chromatography and the oxygenase was eluted with either 3 mmol/l phosphate buffer (pH 7.4) or with 10 mmol/l Tris-HCl buffer (pH 7.6). The oxygenase can also be isolated by filtration of the hemolysate through Sephadex G-100. In healthy persons a mean value of 84.1 +/- 29.7 nmol of porphobilinogen consumed in 30 min per ml of blood was obtained. In patients with hepatic porphyrias (acute intermittent porphyria and porphyria cutanea tarda) the activity of porphobilinogen oxygenase was very low. In the case with acute intermittent porphyria the activity was increased when measured after storage at 4 degrees C but never reached normal values. In the cases of porphyria cutanea tarda, oxygenase activity increased after recovery and reached normal values. In patients with erythropoietic porphyria and in anemias the activity of porphobilinogen oxygenase gave high values. The erythrocyte enzyme was found to be heterogeneous when compared with the enzyme of other sources. It was only partially succinylated and was inactivated after storage for a few days at 4 degrees C. Some preparations showed the usual allosteric kinetics (n = 1.6--2.0), although Michaelian kinetics were also often observed. The enzyme was inhibited by alpha, alpha'-dipyridyl and EDTA as well as by several metals.", "contents": "Porphobilinogen oxygenase from human erythrocytes. Porphobilinogen oxygenase was isolated from red cells of healthy persons and of patients with disturbances in porphyrin metabolism. The hemolysates were purified by DEAE-cellulose chromatography and the oxygenase was eluted with either 3 mmol/l phosphate buffer (pH 7.4) or with 10 mmol/l Tris-HCl buffer (pH 7.6). The oxygenase can also be isolated by filtration of the hemolysate through Sephadex G-100. In healthy persons a mean value of 84.1 +/- 29.7 nmol of porphobilinogen consumed in 30 min per ml of blood was obtained. In patients with hepatic porphyrias (acute intermittent porphyria and porphyria cutanea tarda) the activity of porphobilinogen oxygenase was very low. In the case with acute intermittent porphyria the activity was increased when measured after storage at 4 degrees C but never reached normal values. In the cases of porphyria cutanea tarda, oxygenase activity increased after recovery and reached normal values. In patients with erythropoietic porphyria and in anemias the activity of porphobilinogen oxygenase gave high values. The erythrocyte enzyme was found to be heterogeneous when compared with the enzyme of other sources. It was only partially succinylated and was inactivated after storage for a few days at 4 degrees C. Some preparations showed the usual allosteric kinetics (n = 1.6--2.0), although Michaelian kinetics were also often observed. The enzyme was inhibited by alpha, alpha'-dipyridyl and EDTA as well as by several metals."}
{"id": "PMID:487610", "title": "Differences in lipid and lipoprotein concentrations of capillary and venous blood samples.", "content": "The concentrations of lipids and lipoproteins measured in capillary blood serum taken from young adults were significantly lower than in venous blood, although they correlated satisfactorily. These differences may reflect differences in the morphological and hemodynamic conditions existing either in large veins or in the peripheral circulatory system. It was concluded that venous and capillary blood serum cannot be used interchangeably for these estimations.", "contents": "Differences in lipid and lipoprotein concentrations of capillary and venous blood samples. The concentrations of lipids and lipoproteins measured in capillary blood serum taken from young adults were significantly lower than in venous blood, although they correlated satisfactorily. These differences may reflect differences in the morphological and hemodynamic conditions existing either in large veins or in the peripheral circulatory system. It was concluded that venous and capillary blood serum cannot be used interchangeably for these estimations."}
{"id": "PMID:487611", "title": "Brain specific creatine kinase isoenzyme behavior in rat serum after bile acid, sodium oleate and albumin injection.", "content": "In this investigation we have shown that severe liver disease with cerebral involvement can be followed by the presence of BB creatine kinase isoenzyme in serum. Groups of rats were injected with a bile acid mixture, oleic acid, albumin and normal saline respectively. Bile acids or/and oleic acid induced BB isoenzyme to leak from central nervous tissue and this can be measured in serum. Albumin binding prevented this leakage. The experiments support our hypothesis, based on biochemical findings in human liver failure, that detergent and surface activity properties of the above mentioned components are able to produce this specific brain isoenzyme leakage.", "contents": "Brain specific creatine kinase isoenzyme behavior in rat serum after bile acid, sodium oleate and albumin injection. In this investigation we have shown that severe liver disease with cerebral involvement can be followed by the presence of BB creatine kinase isoenzyme in serum. Groups of rats were injected with a bile acid mixture, oleic acid, albumin and normal saline respectively. Bile acids or/and oleic acid induced BB isoenzyme to leak from central nervous tissue and this can be measured in serum. Albumin binding prevented this leakage. The experiments support our hypothesis, based on biochemical findings in human liver failure, that detergent and surface activity properties of the above mentioned components are able to produce this specific brain isoenzyme leakage."}
{"id": "PMID:487612", "title": "Congenital goitre and hypothyroidism with impaired iodide organification and high thyroid peroxidase concentration.", "content": "A sibship of thirteen subjects whose parents were first cousins was studied for a defect in thyroid hormone synthesis. Five sibs were goitrous and had congenital hypothyroidism. All but one showed a positive perchlorate discharge test (PDT). Three other subjects were goitrous and euthyroid (one with a positive PDT), and the remaining five sibs were euthyroid with a presumably normal thyroid. However, an abnormally exaggerated TSH response to TRH was observed not only in the hypothyroid patients but also in six of the other subjects, indicating a decreased thyroid feedback at the pituitary level in the presence of a normal serum concentration of thyroid hormones. In two hypothyroid patients a normal serum T3, low serum T4 and a low reverse T3 were observed. Microscopic studies of thyroid tissue from three of the sibs disclosed marked cellular hyperplasia with no lymphocytic infiltration anywhere in the tissue. Peroxidase activity was determined on tissue from three sibs by three different assay procedures. It was within the normal range in one patient and was significantly elevated in the other two. There was no evidence for a qualitatively defective peroxidase. The defect in thyroid function in this family does not appear to involve a peroxidase deficiency. Thyroglobulin isolated from the thyroid glands of two of the goitrous, hypothyroid subjects was poorly iodinated but was judged to be normal by immunoreactive and ultracentrifugation procedures. Although the nature of the thyroid metabolic defect in this family was not elucidated, the evidence suggests a genetic defect, probably involving a recessive gene.", "contents": "Congenital goitre and hypothyroidism with impaired iodide organification and high thyroid peroxidase concentration. A sibship of thirteen subjects whose parents were first cousins was studied for a defect in thyroid hormone synthesis. Five sibs were goitrous and had congenital hypothyroidism. All but one showed a positive perchlorate discharge test (PDT). Three other subjects were goitrous and euthyroid (one with a positive PDT), and the remaining five sibs were euthyroid with a presumably normal thyroid. However, an abnormally exaggerated TSH response to TRH was observed not only in the hypothyroid patients but also in six of the other subjects, indicating a decreased thyroid feedback at the pituitary level in the presence of a normal serum concentration of thyroid hormones. In two hypothyroid patients a normal serum T3, low serum T4 and a low reverse T3 were observed. Microscopic studies of thyroid tissue from three of the sibs disclosed marked cellular hyperplasia with no lymphocytic infiltration anywhere in the tissue. Peroxidase activity was determined on tissue from three sibs by three different assay procedures. It was within the normal range in one patient and was significantly elevated in the other two. There was no evidence for a qualitatively defective peroxidase. The defect in thyroid function in this family does not appear to involve a peroxidase deficiency. Thyroglobulin isolated from the thyroid glands of two of the goitrous, hypothyroid subjects was poorly iodinated but was judged to be normal by immunoreactive and ultracentrifugation procedures. Although the nature of the thyroid metabolic defect in this family was not elucidated, the evidence suggests a genetic defect, probably involving a recessive gene."}
{"id": "PMID:487613", "title": "Adrenoleukodystrophy: report of a familial case.", "content": "Adrenoleukodystrophy is an unusual disorder in which progressive diffuse demyelination of the cerebrum is associated with adrenal insufficiency, and which is transmitted as a sex-linked recessive trait. This report describes a 36-year-old-male, who first presented 12 years previously with hypogonadothrophic hypogonadism. It is only more recently that he has developed primary adrenal insufficiency, as well as a neurological disorder characterized by cerebellar, pyramidal tract and cerebral cortical abnormalities. One brother died at age 7 with Schilder's disease, while another brother suffered adrenal insufficiency, progressive mental and neurological deterioration and death at age 38 with post-mortem evidence of adrenoleukodystrophy. Histological studies have revealed cytoplasmic striated inclusions, which proved to be lipid in nature, but the precise defect in lipid metabolism has yet to be clarified.", "contents": "Adrenoleukodystrophy: report of a familial case. Adrenoleukodystrophy is an unusual disorder in which progressive diffuse demyelination of the cerebrum is associated with adrenal insufficiency, and which is transmitted as a sex-linked recessive trait. This report describes a 36-year-old-male, who first presented 12 years previously with hypogonadothrophic hypogonadism. It is only more recently that he has developed primary adrenal insufficiency, as well as a neurological disorder characterized by cerebellar, pyramidal tract and cerebral cortical abnormalities. One brother died at age 7 with Schilder's disease, while another brother suffered adrenal insufficiency, progressive mental and neurological deterioration and death at age 38 with post-mortem evidence of adrenoleukodystrophy. Histological studies have revealed cytoplasmic striated inclusions, which proved to be lipid in nature, but the precise defect in lipid metabolism has yet to be clarified."}
{"id": "PMID:487614", "title": "Effects of the H2 receptor antagonist cimetidine on pituitary hormones in man.", "content": "The effects of the histamine (H2) receptor antagonist cimetidine on serum levels of prolactin (PRL), growth hormone (GH), thyroid stimulating hormone (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH) and cortisol were studied in five normal males. Cimetidine, when given by infusion at the dose of 100 mg/h for 5 h, did not alter adenohypophyseal secretion either basally or after pituitary stimulation with LHRH and TRH. However, 400 mg cimetidine given intravenously as a bolus injection significantly stimulated PRL release in all subjects, without affecting any other measured hormone. A dose-reponse relationship existed, and 200 mg cimetidine seems to be the minimum PRL-releasing dose when given as an intravenous bolus injection. These results suggest that cimetidine releases PRL and that this effect is dose-related, but only when large intravenous injections are given.", "contents": "Effects of the H2 receptor antagonist cimetidine on pituitary hormones in man. The effects of the histamine (H2) receptor antagonist cimetidine on serum levels of prolactin (PRL), growth hormone (GH), thyroid stimulating hormone (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH) and cortisol were studied in five normal males. Cimetidine, when given by infusion at the dose of 100 mg/h for 5 h, did not alter adenohypophyseal secretion either basally or after pituitary stimulation with LHRH and TRH. However, 400 mg cimetidine given intravenously as a bolus injection significantly stimulated PRL release in all subjects, without affecting any other measured hormone. A dose-reponse relationship existed, and 200 mg cimetidine seems to be the minimum PRL-releasing dose when given as an intravenous bolus injection. These results suggest that cimetidine releases PRL and that this effect is dose-related, but only when large intravenous injections are given."}
{"id": "PMID:487615", "title": "Radiation-induced hyperprolactinaemia in a treated acromegalic.", "content": "A 31-year-old acromegalic was normoprolactinaemic after partial removal of her pituitary tumour. The post-operative external pituitary irradiation lowered the mean growth hormone (GH) level from 75 mU/1 to less than 1 mU/1 within 2 years. However, at the same time hyperprolactinaemia developed. These changes in the GH and prolactin levels were confirmed 3 and 4 years after irradiation. The cause of the hyperprolactinaemia was radiation-induced hypothalamic damage. Therefore it is suggested that similar damage may occur in patients receiving external pituitary irradiation for 'prolactinomas' and that this mechanism may contribute to the persistent hyperprolactinaemia observed in such patients.", "contents": "Radiation-induced hyperprolactinaemia in a treated acromegalic. A 31-year-old acromegalic was normoprolactinaemic after partial removal of her pituitary tumour. The post-operative external pituitary irradiation lowered the mean growth hormone (GH) level from 75 mU/1 to less than 1 mU/1 within 2 years. However, at the same time hyperprolactinaemia developed. These changes in the GH and prolactin levels were confirmed 3 and 4 years after irradiation. The cause of the hyperprolactinaemia was radiation-induced hypothalamic damage. Therefore it is suggested that similar damage may occur in patients receiving external pituitary irradiation for 'prolactinomas' and that this mechanism may contribute to the persistent hyperprolactinaemia observed in such patients."}
{"id": "PMID:487616", "title": "Unaltered basal prolactin secretion during short-term oestriol treatment in post-menopausal women.", "content": "Six post-menopausal women were submitted, at 1 month interval, to oral oestriol treatment for 14-day periods at daily doses of 2 and 6 mg. Blood samples were collected for LH, FSH and PRL measurements (by RIA) every other day during treatments as well as during a 14-day control period. Through the effect on LH levels was questionable, oestriol resulted in a clear and significant, although moderate, gradual decrease of mean FSH levels. At the doses used, which are effective in thetreatment of menopausal symptoms, but are devoid of any proliferative effect upon the endometrium, oestriol failed to induce any stimulatory effect on basal PRL secretion in these post-menopausal women.", "contents": "Unaltered basal prolactin secretion during short-term oestriol treatment in post-menopausal women. Six post-menopausal women were submitted, at 1 month interval, to oral oestriol treatment for 14-day periods at daily doses of 2 and 6 mg. Blood samples were collected for LH, FSH and PRL measurements (by RIA) every other day during treatments as well as during a 14-day control period. Through the effect on LH levels was questionable, oestriol resulted in a clear and significant, although moderate, gradual decrease of mean FSH levels. At the doses used, which are effective in thetreatment of menopausal symptoms, but are devoid of any proliferative effect upon the endometrium, oestriol failed to induce any stimulatory effect on basal PRL secretion in these post-menopausal women."}
{"id": "PMID:487617", "title": "Plasma unconjugated oestriol and unconjugated oestradiol-17 beta in late pregnancy: individual fluctuation of concentrations from day to day and every 10 minutes.", "content": "Plasma unconjugated oestriol (E3) and unconjugated oestradiol-17 beta (E2) were determined by radioimmunoassay. In ten normal women in their last month of pregnancy the individual fluctuation of E3 concentrations (mean of the coefficients of variation, CV) from day to day (over 5 days) was 15.6% (range 6.4--26.2%). The individual fluctuation of E2 determined in eight of these women was 16.9 (10.4--25.5) %. In six of the same women who had blood samples collected every 10 min (for 3 h) the individual fluctuation of E3 concentrations was 13.8 (7.0--25.1) %, and of E2 was 16.1 (11.3--26.0) %. The degree of fluctuation of E3 in individuals was in proportion to the mean concentration, unlike E2, suggesting that in clinical practice individual changes in E3 values would be as easy to interpret at low levels as at high levels. The finding that concentrations of plasma unconjugated E3 fluctuate in individuals no more than for E2 or than reported for total E3, and less than reported for 24-h urinary oestrogens, lends practical support to the theoretical preference for the assay of plasma unconjugated E3 to assess fetoplacental function.", "contents": "Plasma unconjugated oestriol and unconjugated oestradiol-17 beta in late pregnancy: individual fluctuation of concentrations from day to day and every 10 minutes. Plasma unconjugated oestriol (E3) and unconjugated oestradiol-17 beta (E2) were determined by radioimmunoassay. In ten normal women in their last month of pregnancy the individual fluctuation of E3 concentrations (mean of the coefficients of variation, CV) from day to day (over 5 days) was 15.6% (range 6.4--26.2%). The individual fluctuation of E2 determined in eight of these women was 16.9 (10.4--25.5) %. In six of the same women who had blood samples collected every 10 min (for 3 h) the individual fluctuation of E3 concentrations was 13.8 (7.0--25.1) %, and of E2 was 16.1 (11.3--26.0) %. The degree of fluctuation of E3 in individuals was in proportion to the mean concentration, unlike E2, suggesting that in clinical practice individual changes in E3 values would be as easy to interpret at low levels as at high levels. The finding that concentrations of plasma unconjugated E3 fluctuate in individuals no more than for E2 or than reported for total E3, and less than reported for 24-h urinary oestrogens, lends practical support to the theoretical preference for the assay of plasma unconjugated E3 to assess fetoplacental function."}
{"id": "PMID:487618", "title": "Plasma dopamine-beta-hydroxylase activity and blood pressure variability in hypertensive man.", "content": "It has been proposed that measurements of plasma dopamine-beta-hydroxylase (DBH) may allow for assessmetn of adrenergic tone and may elucidate a possible neurogenic contribution to essential hypertension. We performed a series of measurements of DBH in fifty-seven normotensive and fifty hypertensive black and white men in order (1) to compare DBH to selected blood pressure patterns and (2) to evalutate the influence of salt intake, posture and race on plasma DBH. Plasma DBH, measured on unrestricted salt intake with subjects supine, was 42 +/- 4 Units/L in white normotensives, greater (P less than 0.05) than black normotensives (26 +/- 6 Units/L). White hypertensives had greater plasma concentrations of DBH than black (35 +/- 3 VS. 24 +/- 5, P less than 0.05). Normotensives did not differ from hypertensives. Dietary salt restriction and upright ambulation increased plasma DBH activity in hypertensives. Although DBH did not correlate directly with blood pressure, high DBH values were associated with lability of blood pressure in hypertensives but not in normotensives. There are two possible explanations for our results: (1) multiple factors influence plasma DBH activity and plasma levels reflect more than adrenergic function, or (2) essential hypertension is a multifactorial disease and excess sympathetic neuronal activity alone is not sufficient to produce sustained hypertension.", "contents": "Plasma dopamine-beta-hydroxylase activity and blood pressure variability in hypertensive man. It has been proposed that measurements of plasma dopamine-beta-hydroxylase (DBH) may allow for assessmetn of adrenergic tone and may elucidate a possible neurogenic contribution to essential hypertension. We performed a series of measurements of DBH in fifty-seven normotensive and fifty hypertensive black and white men in order (1) to compare DBH to selected blood pressure patterns and (2) to evalutate the influence of salt intake, posture and race on plasma DBH. Plasma DBH, measured on unrestricted salt intake with subjects supine, was 42 +/- 4 Units/L in white normotensives, greater (P less than 0.05) than black normotensives (26 +/- 6 Units/L). White hypertensives had greater plasma concentrations of DBH than black (35 +/- 3 VS. 24 +/- 5, P less than 0.05). Normotensives did not differ from hypertensives. Dietary salt restriction and upright ambulation increased plasma DBH activity in hypertensives. Although DBH did not correlate directly with blood pressure, high DBH values were associated with lability of blood pressure in hypertensives but not in normotensives. There are two possible explanations for our results: (1) multiple factors influence plasma DBH activity and plasma levels reflect more than adrenergic function, or (2) essential hypertension is a multifactorial disease and excess sympathetic neuronal activity alone is not sufficient to produce sustained hypertension."}
{"id": "PMID:487619", "title": "Hyperprolactinaemia and hypogonadism in men: response to exogenous gonadotrophins.", "content": "Three male patients with pituitary tumours and marked hyperprolactinaemia were investigated. Their prolactin (PRL) levels ranged from 210 to 2500 ng/ml. The subjects had clinical and laboratory characteristics of hypogonadotrophic hypogonadism. All were treated with human chorionic gonadotrophin (HCG) and in one subject human menopausal gonadotrophin (HMG) was given in addition. In all three patients, despite the persistence of hyperprolactinaemia, serum testosterone had risen to normal levels within 4--17 days after starting HCG. Despite the normal testosterone level, impotence persisted in two patients and the third had persistently decreased libido. The hypogonadism in these patients may be related to an absolute reduction in gonadotroph number secondary to destruction by tumour mass. Alternatively, hyperprolactinaemia may inhibit the synthesis or release of the gonadotrophins or LHRH. Despite hyperprolactinaemia, pharmacological doses of HCG induced testosterone secretion in all these three subjects.", "contents": "Hyperprolactinaemia and hypogonadism in men: response to exogenous gonadotrophins. Three male patients with pituitary tumours and marked hyperprolactinaemia were investigated. Their prolactin (PRL) levels ranged from 210 to 2500 ng/ml. The subjects had clinical and laboratory characteristics of hypogonadotrophic hypogonadism. All were treated with human chorionic gonadotrophin (HCG) and in one subject human menopausal gonadotrophin (HMG) was given in addition. In all three patients, despite the persistence of hyperprolactinaemia, serum testosterone had risen to normal levels within 4--17 days after starting HCG. Despite the normal testosterone level, impotence persisted in two patients and the third had persistently decreased libido. The hypogonadism in these patients may be related to an absolute reduction in gonadotroph number secondary to destruction by tumour mass. Alternatively, hyperprolactinaemia may inhibit the synthesis or release of the gonadotrophins or LHRH. Despite hyperprolactinaemia, pharmacological doses of HCG induced testosterone secretion in all these three subjects."}
{"id": "PMID:487620", "title": "Measurement of 1,25-dihydroxycholecalciferol in man by radioimmunoassay.", "content": "1 alpha, 25-Dihydroxycholecalciferol (1,25-(OH)2D3) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25-(OH)2D3-25-hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (+/- SEM) serum 1,25-(OH)2D3 concentration for a group of healthy adult subjects was 41 +/- 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.", "contents": "Measurement of 1,25-dihydroxycholecalciferol in man by radioimmunoassay. 1 alpha, 25-Dihydroxycholecalciferol (1,25-(OH)2D3) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25-(OH)2D3-25-hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (+/- SEM) serum 1,25-(OH)2D3 concentration for a group of healthy adult subjects was 41 +/- 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure."}
{"id": "PMID:487631", "title": "A radiological study of the adenoid in normal children.", "content": "In this study the radiology of the adenoid is compared in two groups of children: those selected for adenoidectomy and those with a history of head injury who had had a lateral radiograph of the skull. The size of the adenoid was found to be the same in both groups though the nasopharyngeal airway was found to be significantly smaller in the children selected for adenoidectomy. On the basis of measurements in the normal children a lower limit of normal is suggested for the width of the nasopharyngeal airway, this is 2 mm in the case of children below the age of 70 months and 3 mm in the case of children over 70 months old. Only 17% of the children selected for adenoidectomy have abnormally narrow nasopharyngeal airways.", "contents": "A radiological study of the adenoid in normal children. In this study the radiology of the adenoid is compared in two groups of children: those selected for adenoidectomy and those with a history of head injury who had had a lateral radiograph of the skull. The size of the adenoid was found to be the same in both groups though the nasopharyngeal airway was found to be significantly smaller in the children selected for adenoidectomy. On the basis of measurements in the normal children a lower limit of normal is suggested for the width of the nasopharyngeal airway, this is 2 mm in the case of children below the age of 70 months and 3 mm in the case of children over 70 months old. Only 17% of the children selected for adenoidectomy have abnormally narrow nasopharyngeal airways."}
{"id": "PMID:487632", "title": "Mineral content of skeletal bones in otosclerosis.", "content": "In 63 patients with otosclerosis confirmed by operation, the bone mineral content was determined by photon absorptiometry. The bone mineral content and bone mineral concentration were found to be normal, which lends support to the assumption that otosclerosis is a localized disease and not a manifestation of a generalized disorder of the skeletal system.", "contents": "Mineral content of skeletal bones in otosclerosis. In 63 patients with otosclerosis confirmed by operation, the bone mineral content was determined by photon absorptiometry. The bone mineral content and bone mineral concentration were found to be normal, which lends support to the assumption that otosclerosis is a localized disease and not a manifestation of a generalized disorder of the skeletal system."}
{"id": "PMID:487633", "title": "The histopathology of the incus after stapedectomy.", "content": "Twenty-two temporal bones subjected to stapedectomy were examined by light microscopy for histological changes in the long process of the incus. In 19 cases a stainless steel wire technique had been used and in three a polyethylene tube operation had been performed. Twenty healthy unoperated ears were examined as controls. In all operated ears fibrosis and reduced vascularity of the mucosa was noted. In the polyethylene tube group, 1 ear showed the growth of new bone into the lumen of the tube and 1 showed minor resorption of the long process of the incus. In the stainless steel wire group bone resorption of the long process of the incus. In the stainless steel wire group bone resporption was seen in 5 cases and 1 incus also showed an area of necrosis adjacent to the tip; 2 of the 5 incudes showed some loss of the lenticular process. The main factor relating to bony resorption in this group was the presence of other histological changes resulting from postoperative inflammation. These findings represent a critical histological examination; the hearing results obtained appear to be good, although full audiometry is lacking.", "contents": "The histopathology of the incus after stapedectomy. Twenty-two temporal bones subjected to stapedectomy were examined by light microscopy for histological changes in the long process of the incus. In 19 cases a stainless steel wire technique had been used and in three a polyethylene tube operation had been performed. Twenty healthy unoperated ears were examined as controls. In all operated ears fibrosis and reduced vascularity of the mucosa was noted. In the polyethylene tube group, 1 ear showed the growth of new bone into the lumen of the tube and 1 showed minor resorption of the long process of the incus. In the stainless steel wire group bone resorption of the long process of the incus. In the stainless steel wire group bone resporption was seen in 5 cases and 1 incus also showed an area of necrosis adjacent to the tip; 2 of the 5 incudes showed some loss of the lenticular process. The main factor relating to bony resorption in this group was the presence of other histological changes resulting from postoperative inflammation. These findings represent a critical histological examination; the hearing results obtained appear to be good, although full audiometry is lacking."}
{"id": "PMID:487634", "title": "Audio-visual discrimination of speech.", "content": "Tests utilizing audio-visual presentations of speech may have more significance than has hitherto been recognized. Such tests appear to offer more reliable, more realistic and more reproducible measures of communication impairments than either auditory speech discrimination tests, or visual speech discrimination tests.", "contents": "Audio-visual discrimination of speech. Tests utilizing audio-visual presentations of speech may have more significance than has hitherto been recognized. Such tests appear to offer more reliable, more realistic and more reproducible measures of communication impairments than either auditory speech discrimination tests, or visual speech discrimination tests."}
{"id": "PMID:487635", "title": "Prenatal diagnosis of xeroderma pigmentosum (group C) using assays of unscheduled DNA synthesis and postreplication repair.", "content": "The analysis of DNA repair processes is described in two pregnancies at risk for xeroderma pigmentosum. In both cases, excision repair (measured by unscheduled DNA synthesis) and postreplication repair were analyzed. An affected and an unaffected fetus were identified within 3 weeks after amniocentesis. The cells from the affected fetus were found to be deficient in excision DNA repair, whereas the PRR patterns were intermediate between those of normal and PRR deficient cells. This indicates the possibility of prenatal diagnosis of PRR deficient XP patients (XP variants).", "contents": "Prenatal diagnosis of xeroderma pigmentosum (group C) using assays of unscheduled DNA synthesis and postreplication repair. The analysis of DNA repair processes is described in two pregnancies at risk for xeroderma pigmentosum. In both cases, excision repair (measured by unscheduled DNA synthesis) and postreplication repair were analyzed. An affected and an unaffected fetus were identified within 3 weeks after amniocentesis. The cells from the affected fetus were found to be deficient in excision DNA repair, whereas the PRR patterns were intermediate between those of normal and PRR deficient cells. This indicates the possibility of prenatal diagnosis of PRR deficient XP patients (XP variants)."}
{"id": "PMID:487636", "title": "Complex de novo rearrangement of chromosome 9 with clinical features of monosomy 9p syndrome.", "content": "A girl with a complex rearrangement of chromosome 9 is reported. She shows the characteristic clinical features of monosomy 9p syndrome. The rearrangement was apparently preceded by four breaks which resulted in a presumptive tiny deletion of the distal end of the short arm, inversion of the rest of this arm and a proven deletion of the secondary constriction region of the long arm. By means of C-banding, it was possible to demonstrate the paternal origin of the rearranged chromosome 9. Finally, it is shown that the region determining the phenotypic expression of monosomy 9p syndrome is seemingly located at band 9p24.", "contents": "Complex de novo rearrangement of chromosome 9 with clinical features of monosomy 9p syndrome. A girl with a complex rearrangement of chromosome 9 is reported. She shows the characteristic clinical features of monosomy 9p syndrome. The rearrangement was apparently preceded by four breaks which resulted in a presumptive tiny deletion of the distal end of the short arm, inversion of the rest of this arm and a proven deletion of the secondary constriction region of the long arm. By means of C-banding, it was possible to demonstrate the paternal origin of the rearranged chromosome 9. Finally, it is shown that the region determining the phenotypic expression of monosomy 9p syndrome is seemingly located at band 9p24."}
{"id": "PMID:487637", "title": "Features of trisomy 18 and 18p- syndromes in an infant with 45,XY,i(18q).", "content": "An isochromosome for the long arm of chromosome number 18 - 46,XY,i(18q) - was found in an infant who had features of both trisomy 18 and 18p- syndromes. Findings compatible with trisomy 18 included postmature delivery, prominent occiput, severe congenital heart disease, overlapping fingers, and rocker-bottom feet. Those of 18p- syndrome, which frequently resembles Turner syndrome, were downward obliquity to the palpebral fissures, short, webbed neck, low posterior hairline, and widely-spaced nipples. The infant died of heart failure at 3.5 months of age. Parental karyotypes were normal.", "contents": "Features of trisomy 18 and 18p- syndromes in an infant with 45,XY,i(18q). An isochromosome for the long arm of chromosome number 18 - 46,XY,i(18q) - was found in an infant who had features of both trisomy 18 and 18p- syndromes. Findings compatible with trisomy 18 included postmature delivery, prominent occiput, severe congenital heart disease, overlapping fingers, and rocker-bottom feet. Those of 18p- syndrome, which frequently resembles Turner syndrome, were downward obliquity to the palpebral fissures, short, webbed neck, low posterior hairline, and widely-spaced nipples. The infant died of heart failure at 3.5 months of age. Parental karyotypes were normal."}
{"id": "PMID:487638", "title": "Two abnormal clones in the bone marrow cells of a patient with paroxysmal nocturnal hemoglobinuria.", "content": "Paroxysmal nocturnal hemoglobinuria (PNH) is considered to be a clonal disorder, although most investigations have failed to show chromosomal abnormalities. The present patient suffered from PNH and exhibited in bone marrow cells two abnormal clones with 47 chromosomes in addition to cells with 46 chromosomes. One clone showed trisomy 9, a finding previously reported in leukemias and myeloproliferative disorders. Thus, PNH seems to be a clonal myeloproliferative disease.", "contents": "Two abnormal clones in the bone marrow cells of a patient with paroxysmal nocturnal hemoglobinuria. Paroxysmal nocturnal hemoglobinuria (PNH) is considered to be a clonal disorder, although most investigations have failed to show chromosomal abnormalities. The present patient suffered from PNH and exhibited in bone marrow cells two abnormal clones with 47 chromosomes in addition to cells with 46 chromosomes. One clone showed trisomy 9, a finding previously reported in leukemias and myeloproliferative disorders. Thus, PNH seems to be a clonal myeloproliferative disease."}
{"id": "PMID:487639", "title": "Five familial cases with a trisomy 16p syndrome due to translocation.", "content": "A clinical description is given of a syndrome present in three postnatally and two prenatally detected cases with partial trisomy 16p, caused by a familial translocation t(16;21) (p11;q22). The most consistent features of this syndrome are: low birth weight, small head circumference, low-set ears, palato(gnatho)schisis, micrognathia, thumb-agenesis or hypoplasia, hypertonia, overlapping fingers, single umbilical artery, and psychomotor retardation. The clinical picture was identical to that described by Roberts & Duckett (1978) for a single case.", "contents": "Five familial cases with a trisomy 16p syndrome due to translocation. A clinical description is given of a syndrome present in three postnatally and two prenatally detected cases with partial trisomy 16p, caused by a familial translocation t(16;21) (p11;q22). The most consistent features of this syndrome are: low birth weight, small head circumference, low-set ears, palato(gnatho)schisis, micrognathia, thumb-agenesis or hypoplasia, hypertonia, overlapping fingers, single umbilical artery, and psychomotor retardation. The clinical picture was identical to that described by Roberts & Duckett (1978) for a single case."}
{"id": "PMID:487640", "title": "Immunological investigation in adenine phosphoribosyl transferase (APRT) deficiency.", "content": "In a family with partial or total adenine phosphoribosyl transferase (APRT) deficiency, T and B cell function was evaluated by counting rosette-forming cells, by investigating lymphocyte transformation to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), by assaying serum immunoglobulins and determining lymphocyte membrane immunoglobulins by immunofluorescence. Delayed cutaneous hypersensitivity was also assayed. The absence of gross immunological deficiency in the homozygotes, as well as in the heterozygotes, suggests that the APRT-mediated pathway of purine metabolism is relatively unimportant for lymphocyte function.", "contents": "Immunological investigation in adenine phosphoribosyl transferase (APRT) deficiency. In a family with partial or total adenine phosphoribosyl transferase (APRT) deficiency, T and B cell function was evaluated by counting rosette-forming cells, by investigating lymphocyte transformation to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), by assaying serum immunoglobulins and determining lymphocyte membrane immunoglobulins by immunofluorescence. Delayed cutaneous hypersensitivity was also assayed. The absence of gross immunological deficiency in the homozygotes, as well as in the heterozygotes, suggests that the APRT-mediated pathway of purine metabolism is relatively unimportant for lymphocyte function."}
{"id": "PMID:487642", "title": "Circulating immune complexes in various thyroid diseases.", "content": "In a study of 171 patients with various thyroid diseases, circulating immune complexes (CIC), measured by a C1q solid phase radioassay, were detected in 26% of the patients as compared to 8% of the control subjects. CIC were found in 33--55% of the patients with a well defined thyroid autoimmune disorder (Hashimoto's goitre, asymptomatic thyroiditis, spontaneous myxoedema and Graves' disease) and also in the same proportion of patients with diffuse goitre. CIC were correlated to the presence of serum antibodies to microsomal thyroid antigen but not to their titre. No relationship was observed between CIC and the age or sex of the patients and the presence of exophthalmos, or between CIC and the different thyroid function tests or serum anti-thyroglobulin antibodies. CIC were found in untreated patients as well as in those treated with prednisone, methimazole or thyroxine.", "contents": "Circulating immune complexes in various thyroid diseases. In a study of 171 patients with various thyroid diseases, circulating immune complexes (CIC), measured by a C1q solid phase radioassay, were detected in 26% of the patients as compared to 8% of the control subjects. CIC were found in 33--55% of the patients with a well defined thyroid autoimmune disorder (Hashimoto's goitre, asymptomatic thyroiditis, spontaneous myxoedema and Graves' disease) and also in the same proportion of patients with diffuse goitre. CIC were correlated to the presence of serum antibodies to microsomal thyroid antigen but not to their titre. No relationship was observed between CIC and the age or sex of the patients and the presence of exophthalmos, or between CIC and the different thyroid function tests or serum anti-thyroglobulin antibodies. CIC were found in untreated patients as well as in those treated with prednisone, methimazole or thyroxine."}
{"id": "PMID:487643", "title": "Characteristics of complexes for arming and inhibiting effector cells for antibody-dependent cell-mediated cytotoxicity.", "content": "The size of IgG aggregate effective in the inhibition or arming of human effector cells for antibody-dependent cell-mediated cytotoxicity (ADCC) was investigated using heat- and alkalipolymerized rabbit IgG or purified antibody fractionated by gel filtration. In contrast to the inhibition of ADCC against chicken erythrocytes, which was marked when effector cell were pre-incubated with high molecular weight aggregates (19S or greater), small polymers were most effective in arming for cytotoxicity against antigen-coated chicken red cells. Our data also demonstrate that while the cytotoxic potential of armed cells is short-lived and rapidly lost during culture at 37 degrees C but not 4 degrees C, the reduced capacity of these cells to kill antibody-coated targets is not altered by similar incubation at 37 degrees C. The differences in the size of aggregate active in arming and inhibition, and the stability of the two phenomena are compatible with the hypothesis that large aggregates may cause more cross-linking and redistribution of effector cell Fc receptors than small polymers of IgG.", "contents": "Characteristics of complexes for arming and inhibiting effector cells for antibody-dependent cell-mediated cytotoxicity. The size of IgG aggregate effective in the inhibition or arming of human effector cells for antibody-dependent cell-mediated cytotoxicity (ADCC) was investigated using heat- and alkalipolymerized rabbit IgG or purified antibody fractionated by gel filtration. In contrast to the inhibition of ADCC against chicken erythrocytes, which was marked when effector cell were pre-incubated with high molecular weight aggregates (19S or greater), small polymers were most effective in arming for cytotoxicity against antigen-coated chicken red cells. Our data also demonstrate that while the cytotoxic potential of armed cells is short-lived and rapidly lost during culture at 37 degrees C but not 4 degrees C, the reduced capacity of these cells to kill antibody-coated targets is not altered by similar incubation at 37 degrees C. The differences in the size of aggregate active in arming and inhibition, and the stability of the two phenomena are compatible with the hypothesis that large aggregates may cause more cross-linking and redistribution of effector cell Fc receptors than small polymers of IgG."}
{"id": "PMID:487644", "title": "Inhibition of neutrophil migration by sera from patients with rheumatoid arthritis.", "content": "Sera from patients with rheumatoid arthritis inhibited the migration of human neutrophils in 63% (twenty-two out of thirty-five) of the cases tested. The inhibition was not due to a toxic effect of the serum as it was reversed by a chemotactic stimulus. There was a strong correlation between the degree of inhibition of neutrophil migration and the amount of immune complexes present in the sera as determined by the C1q binding activity. It is suggested that the inhibition of neutrophil migration is due to the presence of circulating immune complexes, and that the capacity of immune complexes to inhibit neutrophil migration in vitro may also contribute to the accumulation of neutrophils at sites of immune complex formation in vivo.", "contents": "Inhibition of neutrophil migration by sera from patients with rheumatoid arthritis. Sera from patients with rheumatoid arthritis inhibited the migration of human neutrophils in 63% (twenty-two out of thirty-five) of the cases tested. The inhibition was not due to a toxic effect of the serum as it was reversed by a chemotactic stimulus. There was a strong correlation between the degree of inhibition of neutrophil migration and the amount of immune complexes present in the sera as determined by the C1q binding activity. It is suggested that the inhibition of neutrophil migration is due to the presence of circulating immune complexes, and that the capacity of immune complexes to inhibit neutrophil migration in vitro may also contribute to the accumulation of neutrophils at sites of immune complex formation in vivo."}
{"id": "PMID:487645", "title": "A longitudinal study of immunological parameters in multiple sclerosis: cell-mediated immunity and complement profiles.", "content": "In a 1 year longitudinal study of twenty-two patients with multiple sclerosis (MS) and twenty-one normal control subjects, peripheral blood leucocytes were stimulated with lipoplysaccharide (LPS), a B cell mitogen, and phytohaemagglutinin (PHA) a T cell mitogen. EA- and EAC-rosette formation was also performed to assay null and B cells respectively, and the serum concentration of the C3 component of complement was determined. For ten multiple sclerosis (MS) patients with stationary phases of the disease, percentages of lymphocytes significantly lower than normal were found with normal leucocyte counts in the peripheral blood. Lymphocyte stimulation by PHA was significantly lower than normal in stable MS, while neither LPS stimulation nor EA- and EAC-rosette formation differed significantly from the controls. The C3 serum concentration was found to be significantly higher than normal. For nine out of twelve MS patients with a fluctuating clinical course, the C3 concentration was elevated during remission, but dropped to normal levels during relapse. None of the other parameters studied could be correlated with the clinical course.", "contents": "A longitudinal study of immunological parameters in multiple sclerosis: cell-mediated immunity and complement profiles. In a 1 year longitudinal study of twenty-two patients with multiple sclerosis (MS) and twenty-one normal control subjects, peripheral blood leucocytes were stimulated with lipoplysaccharide (LPS), a B cell mitogen, and phytohaemagglutinin (PHA) a T cell mitogen. EA- and EAC-rosette formation was also performed to assay null and B cells respectively, and the serum concentration of the C3 component of complement was determined. For ten multiple sclerosis (MS) patients with stationary phases of the disease, percentages of lymphocytes significantly lower than normal were found with normal leucocyte counts in the peripheral blood. Lymphocyte stimulation by PHA was significantly lower than normal in stable MS, while neither LPS stimulation nor EA- and EAC-rosette formation differed significantly from the controls. The C3 serum concentration was found to be significantly higher than normal. For nine out of twelve MS patients with a fluctuating clinical course, the C3 concentration was elevated during remission, but dropped to normal levels during relapse. None of the other parameters studied could be correlated with the clinical course."}
{"id": "PMID:487646", "title": "The spleen is required for the suppression of experimental allergic encephalomyelitis by prostaglandin precursors.", "content": "In this paper we report a study of the effects of splenectomy on the immunosuppressive action of essential fatty acids (EFA) which is thought to be mediated through prostaglandins (PG) produced in the spleen. Experimental allergic encephalomyelitis (EAE) was induced in normal, splenectomized and sham splenectomized Lewis rats. EFA were administered orally, the animals in the control groups being treated with liquid paraffin. Treatment with EFA significantly suppressed clinical disease in those animals in which EAE was induced by the inoculation of central nervous system material of guinea-pigs or by passive transfer by Con A-stimulated spleen cells. Splenectomy abrogated the suppressive effect of EFA. This observation, together with previous results showing the abrogation of EFA immunosuppression by an inhibitor of the biosynthesis of PG from EFA, led us to postulate a close relationship between EFA, PG and a splenic factor suppressing immunopathological mechanisms in EAE.", "contents": "The spleen is required for the suppression of experimental allergic encephalomyelitis by prostaglandin precursors. In this paper we report a study of the effects of splenectomy on the immunosuppressive action of essential fatty acids (EFA) which is thought to be mediated through prostaglandins (PG) produced in the spleen. Experimental allergic encephalomyelitis (EAE) was induced in normal, splenectomized and sham splenectomized Lewis rats. EFA were administered orally, the animals in the control groups being treated with liquid paraffin. Treatment with EFA significantly suppressed clinical disease in those animals in which EAE was induced by the inoculation of central nervous system material of guinea-pigs or by passive transfer by Con A-stimulated spleen cells. Splenectomy abrogated the suppressive effect of EFA. This observation, together with previous results showing the abrogation of EFA immunosuppression by an inhibitor of the biosynthesis of PG from EFA, led us to postulate a close relationship between EFA, PG and a splenic factor suppressing immunopathological mechanisms in EAE."}
{"id": "PMID:487647", "title": "Effects of repeated Corynebacterium parvum and BCG therapy on immune parameters: a weekly study of melanoma patients II. Changes in serum immunoglobulins and lymphoid cell subpopulations.", "content": "Two groups, each of eight patients, with metastatic melanoma were immunized with either C. parvum or BCG at 3-weekly intervals. The serum IgG, IgA, IgM levels, E-rosettes, EA-rosetts, sIg-bearing cells, 'null cells' and lymphocyte blastogenesis at four PHA concentrations were assayed, before immunization and weekly thereafter for 8 weeks. The pre-immunization E-rosette values were significantly reduced, with a significant increase in 'null cell' values and EA-rosette percentages, when compared with healthy controls. Following C. parvum immunization, significant increases occurred in E-rosette percentages with decreases in EA-rosette and 'null cell' counts. A significant reduction in 'null cell' values also occurred with BCG. Other parameters including PHA blastogenesis did not exhibit statistically significant changes. Different methods of expressing the results (particularly of blastogenesis data) were compared, as were the similarities between the present investigation and a study of the effects of a single immunization. The relationship between the subpopulation changes and lymphocyte cytotoxicity described in an earlier article (Thatcher, Swindell & Crowther, 1979a) is discussed.", "contents": "Effects of repeated Corynebacterium parvum and BCG therapy on immune parameters: a weekly study of melanoma patients II. Changes in serum immunoglobulins and lymphoid cell subpopulations. Two groups, each of eight patients, with metastatic melanoma were immunized with either C. parvum or BCG at 3-weekly intervals. The serum IgG, IgA, IgM levels, E-rosettes, EA-rosetts, sIg-bearing cells, 'null cells' and lymphocyte blastogenesis at four PHA concentrations were assayed, before immunization and weekly thereafter for 8 weeks. The pre-immunization E-rosette values were significantly reduced, with a significant increase in 'null cell' values and EA-rosette percentages, when compared with healthy controls. Following C. parvum immunization, significant increases occurred in E-rosette percentages with decreases in EA-rosette and 'null cell' counts. A significant reduction in 'null cell' values also occurred with BCG. Other parameters including PHA blastogenesis did not exhibit statistically significant changes. Different methods of expressing the results (particularly of blastogenesis data) were compared, as were the similarities between the present investigation and a study of the effects of a single immunization. The relationship between the subpopulation changes and lymphocyte cytotoxicity described in an earlier article (Thatcher, Swindell & Crowther, 1979a) is discussed."}
{"id": "PMID:487648", "title": "Transfer factor therapy in hyperimmunoglobulinaemia E syndrome.", "content": "The therapeutic effect of transfer factor (TF) from healthy donors was investigated in two children with extensive intractable atopic dermatitis, recurrent pyogenic skin infections, hyperimmunoglobulinaemia E, defective neutrophil chemotaxis and depressed cell-mediated immunity. Striking clinical improvement was noted in both patients with disappearance of skin infections, pruritus and eczema. No new lesions have occurred 13 months after the completion of therapy in the first patient but a few new atopic lesions have reappeared after 8 months in the second. Both patients are off steroids and antibiotics. Transfer factor administration did not influence the T cell rosette number or the lymphocyte blastic transformation response, but it did cause conversion of the skin-test reactivity in both patients and correction of polymorphonuclear chemotaxis in one of them. Non clinical side-effects were noted but marked and persistent rise of serum IgE was observed in both patients. Our data suggest that patients with hyper-IgE syndrome may be benefited by TF therapy and they lend further support to the notion that T lymphocyte deficiency may be the basis of the eczema in this syndrome.", "contents": "Transfer factor therapy in hyperimmunoglobulinaemia E syndrome. The therapeutic effect of transfer factor (TF) from healthy donors was investigated in two children with extensive intractable atopic dermatitis, recurrent pyogenic skin infections, hyperimmunoglobulinaemia E, defective neutrophil chemotaxis and depressed cell-mediated immunity. Striking clinical improvement was noted in both patients with disappearance of skin infections, pruritus and eczema. No new lesions have occurred 13 months after the completion of therapy in the first patient but a few new atopic lesions have reappeared after 8 months in the second. Both patients are off steroids and antibiotics. Transfer factor administration did not influence the T cell rosette number or the lymphocyte blastic transformation response, but it did cause conversion of the skin-test reactivity in both patients and correction of polymorphonuclear chemotaxis in one of them. Non clinical side-effects were noted but marked and persistent rise of serum IgE was observed in both patients. Our data suggest that patients with hyper-IgE syndrome may be benefited by TF therapy and they lend further support to the notion that T lymphocyte deficiency may be the basis of the eczema in this syndrome."}
{"id": "PMID:487649", "title": "Re-examination of the EA rosette assay (Ripley) for Fc receptor leucocytes.", "content": "Numerous investigations has utilized rosette formation with Ripley antibody-coated human erythrocytes (EA) to identify or deplete Fc receptor-bearing K lymphocytes in whole mononuclear cell preparations. This study examines the interaction between Ripley EA and purified preparations of human lymphocytes, monocytes and neutrophils and demonstrates that this technique is not specific for K lymphocytes. Indeed, 100% of blood monocytes rosette these EA target cells. Moreover, data from both rosetting studies and antibody-dependent cytotoxicity (ADCC) reactions suggest that the avidity of Ripley EA is actually greater for monocytes than for lymphocytes. In contrast to previous reports, 100% human neutrophils were found to possess Fc receptors, as determined by their ability to rosette Ripley EA. Thus, the extent of rosetting and ADCC by all three Fc receptor-bearing leucocytes depends significantly on the degree of antibody sensitization with neutrophils requiring the greatest, and monocytes the least, amount of target-bound antibody for Fc receptor-mediated interaction.", "contents": "Re-examination of the EA rosette assay (Ripley) for Fc receptor leucocytes. Numerous investigations has utilized rosette formation with Ripley antibody-coated human erythrocytes (EA) to identify or deplete Fc receptor-bearing K lymphocytes in whole mononuclear cell preparations. This study examines the interaction between Ripley EA and purified preparations of human lymphocytes, monocytes and neutrophils and demonstrates that this technique is not specific for K lymphocytes. Indeed, 100% of blood monocytes rosette these EA target cells. Moreover, data from both rosetting studies and antibody-dependent cytotoxicity (ADCC) reactions suggest that the avidity of Ripley EA is actually greater for monocytes than for lymphocytes. In contrast to previous reports, 100% human neutrophils were found to possess Fc receptors, as determined by their ability to rosette Ripley EA. Thus, the extent of rosetting and ADCC by all three Fc receptor-bearing leucocytes depends significantly on the degree of antibody sensitization with neutrophils requiring the greatest, and monocytes the least, amount of target-bound antibody for Fc receptor-mediated interaction."}
{"id": "PMID:487651", "title": "Corticosteroid enhancement of immunoglobulin synthesis by pokeweed mitogen-stimulated human lymphocytes.", "content": "The effects of the addition in vitro of corticosteroid on pokeweed mitogen (PWM) induced Ig synthesis by human peripheral blood lymphocytes were studied. IgG in supernatants produced under standardized culture conditions was measured by double antibody radioimmunoassay. The addition of 10(-6)M prednisolone caused a remarkable enhancement of PWM-stimulated IgG synthesis beginning at day 4 of culture and increasing at a faster rate than that in cultures with PWM alone. 10(-6)M prednisolone resulted in a geometric mean enhancement of 5.6-fold of PWM-stimulated IgG synthesis in all twenty-five normal controls studied. This enhancement occurred up to 3 days after the addition of PWM. 10(-6)M and 10(-5)M prednisolone resulted in significantly greater enhancement of PWM-stimulated IgG synthesis than 10(-7)M prednisolone. Hydrocortisone, prednisolone, methylprednisolone, betamethasone and dexamethasone at 10(-6)M were all equally effective in the enhancement of PWM-induced IgG synthesis.", "contents": "Corticosteroid enhancement of immunoglobulin synthesis by pokeweed mitogen-stimulated human lymphocytes. The effects of the addition in vitro of corticosteroid on pokeweed mitogen (PWM) induced Ig synthesis by human peripheral blood lymphocytes were studied. IgG in supernatants produced under standardized culture conditions was measured by double antibody radioimmunoassay. The addition of 10(-6)M prednisolone caused a remarkable enhancement of PWM-stimulated IgG synthesis beginning at day 4 of culture and increasing at a faster rate than that in cultures with PWM alone. 10(-6)M prednisolone resulted in a geometric mean enhancement of 5.6-fold of PWM-stimulated IgG synthesis in all twenty-five normal controls studied. This enhancement occurred up to 3 days after the addition of PWM. 10(-6)M and 10(-5)M prednisolone resulted in significantly greater enhancement of PWM-stimulated IgG synthesis than 10(-7)M prednisolone. Hydrocortisone, prednisolone, methylprednisolone, betamethasone and dexamethasone at 10(-6)M were all equally effective in the enhancement of PWM-induced IgG synthesis."}
{"id": "PMID:487652", "title": "Peripheral lymphocytes changes in the anamnestic response to tetanus toxoid challenge.", "content": "Few data are available on the blood lymphocyte response to revaccination in man. The anamnestic response to tetanus toxoid challenge was evaluated by a variety of techniques during the first week after revaccination. Out of twenty subjects used, eight were evaluated before and 5 days after the injections (days 1--8). Analytical cell electrophoresis showed important variation in the B and two T lymphocyte populations. The B cell percentages, assessed by EAC-rosettes and electrophoretic mobility, were found to decrease by days 2 and 3, and return to former levels by day 8, when a rise in specific antibodies was detected. A similar response was found in the T1 population generally considered to be composed of low affinity E-rosette-forming cells. Conversely, a significant increase (50--100%) in circulating T2 lymphocytes (active rosette-forming cells) was found by days 2 and 3, followed by a rapid decrease of these 'differenciated' cells. The increase in the T2 lymphocytes appeared earlier in skin test positive subjects. These changes were correlated with E-rosettes, mitogen stimulation, peripheral leucocyte migration inhibition and transformation in the presence of the antigen. EA-IgG rosettes and ADCC varied similarly. These results may indicate a significant non-specific cell mobilization following revaccination.", "contents": "Peripheral lymphocytes changes in the anamnestic response to tetanus toxoid challenge. Few data are available on the blood lymphocyte response to revaccination in man. The anamnestic response to tetanus toxoid challenge was evaluated by a variety of techniques during the first week after revaccination. Out of twenty subjects used, eight were evaluated before and 5 days after the injections (days 1--8). Analytical cell electrophoresis showed important variation in the B and two T lymphocyte populations. The B cell percentages, assessed by EAC-rosettes and electrophoretic mobility, were found to decrease by days 2 and 3, and return to former levels by day 8, when a rise in specific antibodies was detected. A similar response was found in the T1 population generally considered to be composed of low affinity E-rosette-forming cells. Conversely, a significant increase (50--100%) in circulating T2 lymphocytes (active rosette-forming cells) was found by days 2 and 3, followed by a rapid decrease of these 'differenciated' cells. The increase in the T2 lymphocytes appeared earlier in skin test positive subjects. These changes were correlated with E-rosettes, mitogen stimulation, peripheral leucocyte migration inhibition and transformation in the presence of the antigen. EA-IgG rosettes and ADCC varied similarly. These results may indicate a significant non-specific cell mobilization following revaccination."}
{"id": "PMID:487653", "title": "Proteolysis of human IgG by human polymorphonuclear leucocyte elastase produces an Fc fragment with in vitro biological activity.", "content": "The Fc fragment derived by proteolysis of human IgG by human polymorphonuclear leucocyte (PMN) elastase was tested for in vitro biological activity. This fragment could attach to the specific IgG receptor of Staphylococcus aureus Cowan I cells (protein A) and could be eluted from the cells with dissociating buffer. Taking advantage of this attachment, it was shown that the Fc fragment is capable of attaching to the antigen-combining site of an IgM rheumatoid factor and can bind to the Fc receptor of human PMN. A similar fragment produced in vivo at sites of inflammation could play a role in regulating the inflammatory response.", "contents": "Proteolysis of human IgG by human polymorphonuclear leucocyte elastase produces an Fc fragment with in vitro biological activity. The Fc fragment derived by proteolysis of human IgG by human polymorphonuclear leucocyte (PMN) elastase was tested for in vitro biological activity. This fragment could attach to the specific IgG receptor of Staphylococcus aureus Cowan I cells (protein A) and could be eluted from the cells with dissociating buffer. Taking advantage of this attachment, it was shown that the Fc fragment is capable of attaching to the antigen-combining site of an IgM rheumatoid factor and can bind to the Fc receptor of human PMN. A similar fragment produced in vivo at sites of inflammation could play a role in regulating the inflammatory response."}
{"id": "PMID:487654", "title": "The reversal of surgically induced reticuloendothelial depression.", "content": "The function of the reticuloendothelial (RE) system may be depressed by surgical operation. As this system is an important defence mechanism against tumour growth and metastasis, it would be an advantage if this depression could be prevented by the administration of a suitable agent. The agents studied in this case were levamisole, Corynebacterium parvum (C. parvum) and glucan. As a measure of RE function a carbon clearance test was performed in rats, 1 hr after partial colectomy, animals having received pre-operative treatment with one of these substances. The results show that operation produced a significant fall in clearance (P less than 0.001). Both C. parvum and glucan were very effective in preventing any fall of clearance following operation (P less than 0.01). Both of these produced marked RE stimulation and values in animals given these agents remained above control values even after surgery. Levamisole had some effect in preventing the RE depression (P less than 0.02), but was not as effective as either C. parvum or glucan.", "contents": "The reversal of surgically induced reticuloendothelial depression. The function of the reticuloendothelial (RE) system may be depressed by surgical operation. As this system is an important defence mechanism against tumour growth and metastasis, it would be an advantage if this depression could be prevented by the administration of a suitable agent. The agents studied in this case were levamisole, Corynebacterium parvum (C. parvum) and glucan. As a measure of RE function a carbon clearance test was performed in rats, 1 hr after partial colectomy, animals having received pre-operative treatment with one of these substances. The results show that operation produced a significant fall in clearance (P less than 0.001). Both C. parvum and glucan were very effective in preventing any fall of clearance following operation (P less than 0.01). Both of these produced marked RE stimulation and values in animals given these agents remained above control values even after surgery. Levamisole had some effect in preventing the RE depression (P less than 0.02), but was not as effective as either C. parvum or glucan."}
{"id": "PMID:487656", "title": "Circulating immune complexes in normal pregnant women and in some conditions complicating pregnancy.", "content": "The polyethylene-glycol (PEG) precipitation assay was used to examine the sera of sixty-nine pregnant women, thirty with normal pregnancies, ten with toxaemia of pregnancy, eleven with pregnancy complicated by diabetes and eighteen with case histories of recurrent abortions in order to find evidence of eventual circulating immune complexes (CIC). CIC were not seen in normal pregnancies, but were found in two of the toxaemic group, in two of those with recurrent abortions and in two of those with diabetes. After delivery, these six positive cases were all negative. The presence of CIC may have a determining role in the pathogenesis of some of the cases of the above conditions.", "contents": "Circulating immune complexes in normal pregnant women and in some conditions complicating pregnancy. The polyethylene-glycol (PEG) precipitation assay was used to examine the sera of sixty-nine pregnant women, thirty with normal pregnancies, ten with toxaemia of pregnancy, eleven with pregnancy complicated by diabetes and eighteen with case histories of recurrent abortions in order to find evidence of eventual circulating immune complexes (CIC). CIC were not seen in normal pregnancies, but were found in two of the toxaemic group, in two of those with recurrent abortions and in two of those with diabetes. After delivery, these six positive cases were all negative. The presence of CIC may have a determining role in the pathogenesis of some of the cases of the above conditions."}
{"id": "PMID:487657", "title": "The effect of defibrination on macrophage participation in rabbit nephrotoxic nephritis: studies using glomerular culture and electronmicroscopy.", "content": "Recent studies in experimental crescentic glomerulonephritis, using the technique of glomerular culture, have shown that the macrophage is a major cell type present within the glomeruli and developing crescents. It has been suggested that their accumulation is a consequence of glomerular fibrin deposition. The effect of defibrination with ancrod on the cellular events occurring in experimental crescentic glomerulonephritis in the rabbit was therefore assessed in this disease using the techniques of culture of isolated glomeruli, electronmicroscopy or renal tissue, and light microscopy. Defibrinated animals developed only minimal renal impairment, virtually no fibrin deposition in Bowman's Space and only a mild degree of crescent formation, in contrast to the severe renal failure, fibrin deposition and crescent formation that occurred in the untreated animals. The culture of isolated glomeruli and electronmicroscopy of intact renal tissue demonstrated large numbers of macrophages within and emerging from glomeruli of both defibrinated and untreated animals. However, only in untreated animals were macrophages seen to migrate into Bowman's Space, phagocytose fibrin, transform into epithelioid cells and accumulate to form crescents. These studies suggest that fibrin deposition in Bowman's Space is the major stimulus to the macrophage migration from capillary loops and accumulation in Bowman's Space. However, fibrin deposition does not appear to be the stimulus to macrophage accumulation within capillary loops as this event was not affected by defibrination.", "contents": "The effect of defibrination on macrophage participation in rabbit nephrotoxic nephritis: studies using glomerular culture and electronmicroscopy. Recent studies in experimental crescentic glomerulonephritis, using the technique of glomerular culture, have shown that the macrophage is a major cell type present within the glomeruli and developing crescents. It has been suggested that their accumulation is a consequence of glomerular fibrin deposition. The effect of defibrination with ancrod on the cellular events occurring in experimental crescentic glomerulonephritis in the rabbit was therefore assessed in this disease using the techniques of culture of isolated glomeruli, electronmicroscopy or renal tissue, and light microscopy. Defibrinated animals developed only minimal renal impairment, virtually no fibrin deposition in Bowman's Space and only a mild degree of crescent formation, in contrast to the severe renal failure, fibrin deposition and crescent formation that occurred in the untreated animals. The culture of isolated glomeruli and electronmicroscopy of intact renal tissue demonstrated large numbers of macrophages within and emerging from glomeruli of both defibrinated and untreated animals. However, only in untreated animals were macrophages seen to migrate into Bowman's Space, phagocytose fibrin, transform into epithelioid cells and accumulate to form crescents. These studies suggest that fibrin deposition in Bowman's Space is the major stimulus to the macrophage migration from capillary loops and accumulation in Bowman's Space. However, fibrin deposition does not appear to be the stimulus to macrophage accumulation within capillary loops as this event was not affected by defibrination."}
{"id": "PMID:487658", "title": "Heterogeneity of anti-mitochondrial antibodies: characterization and separation of the antigen associated with the pseudolupus erythematosus syndrome.", "content": "It could be shown that anti-mitochondria antibodies (AMA) found in drug-induced pseudolupus erythematosus syndrome (PLE) had a different specificity from those found previously in primary biliary cirrhosis (PBC). The PLE antigen could be easily separated from the PBC antigen either by isopycnic sucrose density gradient centrifugation of cytoplasmic extracts (supernatant 40) or purified sonicated rat liver or kidney mitochondria. The PLE antigen was firmly membrane-bound and, in contrast to the PBC antigen, not solubilized by treatment with various salts or enzymes. The ATP-ase complex, the probable target antigen of PBC-specific antibodies, did not react with sera from patients with the PLE syndrome. There is evidence that the PLE-associated antibodies (M3) occur exclusively in patients who have been sensitized to derivatives of pyrazolone or their metabolites, indicating that PLE antibodies may be specific markers for this type of drug allergy.", "contents": "Heterogeneity of anti-mitochondrial antibodies: characterization and separation of the antigen associated with the pseudolupus erythematosus syndrome. It could be shown that anti-mitochondria antibodies (AMA) found in drug-induced pseudolupus erythematosus syndrome (PLE) had a different specificity from those found previously in primary biliary cirrhosis (PBC). The PLE antigen could be easily separated from the PBC antigen either by isopycnic sucrose density gradient centrifugation of cytoplasmic extracts (supernatant 40) or purified sonicated rat liver or kidney mitochondria. The PLE antigen was firmly membrane-bound and, in contrast to the PBC antigen, not solubilized by treatment with various salts or enzymes. The ATP-ase complex, the probable target antigen of PBC-specific antibodies, did not react with sera from patients with the PLE syndrome. There is evidence that the PLE-associated antibodies (M3) occur exclusively in patients who have been sensitized to derivatives of pyrazolone or their metabolites, indicating that PLE antibodies may be specific markers for this type of drug allergy."}
{"id": "PMID:487659", "title": "Regulation of secondary antibody responses in rodents. I. Potentiation of IgG production by cyclophosphamide.", "content": "This paper describes the effects of a single dose of cyclophosphamide on specific IgG production in rats during an established secondary immune response. (PVG X Agus)F1 rats were immunized twice (days 0 and 28) with chicken erythrocytes (CRBC), received cyclophosphamide (100 mg/m2 of body surface area) on day 33 and were killed 8 days later. The production of anti-CRBC IgG antibodies was assessed by testing the supernatants of spleen cell cultures in a cytotoxicity assay with 51Cr-labelled CRBC as target cells and normal rat spleen cells as effector cells. In observations of fifty-nine pairs of treated and untreated rats from eight separate experiments, the administration of cyclophosphamide resulted in: (1) a decrease in the number of spleen cell to a median of 10(8.63) from a median of 10(8.7) (P less than 0.0025); (2) an increase in the anti-CRBC IgG antibody titre of the supernatants of cultured spleen cells to a median of 10(0.67) from a median of 10(0.27 (P less than 0.0025); and (3) the calculated anti-CRBC. IgG antibody production per spleen to be increased in the drug-treated rats to a median of 10(2.26) from a median of 10(2.0) (P less than 0.005). In a cyclophosphamide dose-response study, it was shown that some enhancement of antibody production was induced by doses between 12.5 and 50 mg/m2 and consistently elevated levels of antibody production were associated with doses between 100 and 400 mg/m2.", "contents": "Regulation of secondary antibody responses in rodents. I. Potentiation of IgG production by cyclophosphamide. This paper describes the effects of a single dose of cyclophosphamide on specific IgG production in rats during an established secondary immune response. (PVG X Agus)F1 rats were immunized twice (days 0 and 28) with chicken erythrocytes (CRBC), received cyclophosphamide (100 mg/m2 of body surface area) on day 33 and were killed 8 days later. The production of anti-CRBC IgG antibodies was assessed by testing the supernatants of spleen cell cultures in a cytotoxicity assay with 51Cr-labelled CRBC as target cells and normal rat spleen cells as effector cells. In observations of fifty-nine pairs of treated and untreated rats from eight separate experiments, the administration of cyclophosphamide resulted in: (1) a decrease in the number of spleen cell to a median of 10(8.63) from a median of 10(8.7) (P less than 0.0025); (2) an increase in the anti-CRBC IgG antibody titre of the supernatants of cultured spleen cells to a median of 10(0.67) from a median of 10(0.27 (P less than 0.0025); and (3) the calculated anti-CRBC. IgG antibody production per spleen to be increased in the drug-treated rats to a median of 10(2.26) from a median of 10(2.0) (P less than 0.005). In a cyclophosphamide dose-response study, it was shown that some enhancement of antibody production was induced by doses between 12.5 and 50 mg/m2 and consistently elevated levels of antibody production were associated with doses between 100 and 400 mg/m2."}
{"id": "PMID:487660", "title": "Long-term deficiency of essential fatty acids in rats and its effect on brain recovery.", "content": "1. Nineteen-day old rats were fed an essential fatty acid deficient diet for 37 weeks, thereby making them essential fatty acid deficient for at least 24 weeks. The weights of their brains were 33% less than those of rats on control diets. 2. When the deficient rats were rehabilitated for ten weeks on a diet containing 5% by weight corn oil as a source of essential fatty acids, the weights of their brains were still 30% less than those of the controls. 3. Analysis of the brain lipids showed that, there was a large reduction in the proportions of cerebrosides and sphingomyelin in essential fatty acid deficiency. 4. Essential fatty acid deficiency was also characterised by the appearance of a high proportion of eicosatrienoic acid (20:3) and the reduction of arachidonic acid (20:4) and the other essential fatty acids. 5. Rehabilitation for a period of 10 weeks did not reverse these changes in brain composition. 6. It is considered that the feeding of essential fatty acid deficient diet to rats for a prolonged period of time causes retardation of brain maturity and makes the brain resistant to later dietary manipulations.", "contents": "Long-term deficiency of essential fatty acids in rats and its effect on brain recovery. 1. Nineteen-day old rats were fed an essential fatty acid deficient diet for 37 weeks, thereby making them essential fatty acid deficient for at least 24 weeks. The weights of their brains were 33% less than those of rats on control diets. 2. When the deficient rats were rehabilitated for ten weeks on a diet containing 5% by weight corn oil as a source of essential fatty acids, the weights of their brains were still 30% less than those of the controls. 3. Analysis of the brain lipids showed that, there was a large reduction in the proportions of cerebrosides and sphingomyelin in essential fatty acid deficiency. 4. Essential fatty acid deficiency was also characterised by the appearance of a high proportion of eicosatrienoic acid (20:3) and the reduction of arachidonic acid (20:4) and the other essential fatty acids. 5. Rehabilitation for a period of 10 weeks did not reverse these changes in brain composition. 6. It is considered that the feeding of essential fatty acid deficient diet to rats for a prolonged period of time causes retardation of brain maturity and makes the brain resistant to later dietary manipulations."}
{"id": "PMID:487661", "title": "The influence of intraperitoneally injected 6-hydroxydopamine on electroshock seizure in chicks and rats.", "content": "1. 6-Hydroxydopamine, injected intraperitoneally in rats and chicks, did not induce spontaneous seizures but produced significant alterations in the threshold to electroshock seizure in chicks; the particular effects were dose-dependent and time-dependent. 2. Administration of 6-hydroxydopamine to 3 day old chicks and rats in the first and third days after birth resulted in an increase in the proportion exhibiting tonic seizure with electroshock when tested after 10-12 weeks. 3. When 6-hydroxydopamine was injected intraperitoneally into adult rats and cocks, there was no significant alteration in seizure threshold. 4. The results suggest that 6-hydroxydopamine penetrates the central nervous system of young chicks and rats and that adrenergic mechanisms are probably involved in modulating seizure mechanisms in both the chick and rat.", "contents": "The influence of intraperitoneally injected 6-hydroxydopamine on electroshock seizure in chicks and rats. 1. 6-Hydroxydopamine, injected intraperitoneally in rats and chicks, did not induce spontaneous seizures but produced significant alterations in the threshold to electroshock seizure in chicks; the particular effects were dose-dependent and time-dependent. 2. Administration of 6-hydroxydopamine to 3 day old chicks and rats in the first and third days after birth resulted in an increase in the proportion exhibiting tonic seizure with electroshock when tested after 10-12 weeks. 3. When 6-hydroxydopamine was injected intraperitoneally into adult rats and cocks, there was no significant alteration in seizure threshold. 4. The results suggest that 6-hydroxydopamine penetrates the central nervous system of young chicks and rats and that adrenergic mechanisms are probably involved in modulating seizure mechanisms in both the chick and rat."}
{"id": "PMID:487688", "title": "Effects of atenolol and propranolol when added to long-term antihypertensive diuretic therapy.", "content": "The antihypertensive effects of atenolol and propranolol were compared in a double-blind crossover study of 19 patients with essential hypertension (World Health Organization, I and II) who were receiving long-term diuretic treatment (chlorthalidone, 50 mg daily) during the study. After a 3-wk placebo period, a beta-adrenergic antagonist was administered once daily (atenolol, 50 mg daily, or propranolol, 80 mg daily) for a week. If the MAP was more than 108 mm Hg at the end of the week, dosage of the beta-blocker was doubled the following week; when necessary, doubling was repeated to a maximum dose of 640 mg propranolol and 400 mg atenolol daily. Fifty milligrams atenolol had a greater effect than 80 mg propranolol and was as effective as 160 mg propranolol. The dose-response curve flattened off after 160 mg propranolol and 50 mg atenolol daily. The two highest doses of atenolol lowered MAP more than the highest doses of propranolo. Heart rate slowing was the same for both drugs and did not correlate with the fall in blood pressure. PRA was suppressed by all doses of propranolol, whereas atenolol suppressed PRA only at the 2 highest doses, (200 and 400 mg daily). With the lower propranolol doses, the percent MAP change correlated weakly with the percent PRA change (80 mg--r = 0.41, p less than 0.1; 160 mg--r = 0.64, p less than 0.05). Side effects were minimal, and were noted only with 640 mg propranolol; with this exception, the percentage of patients with no complaints rose when placebo was replaced by beta-blockers.", "contents": "Effects of atenolol and propranolol when added to long-term antihypertensive diuretic therapy. The antihypertensive effects of atenolol and propranolol were compared in a double-blind crossover study of 19 patients with essential hypertension (World Health Organization, I and II) who were receiving long-term diuretic treatment (chlorthalidone, 50 mg daily) during the study. After a 3-wk placebo period, a beta-adrenergic antagonist was administered once daily (atenolol, 50 mg daily, or propranolol, 80 mg daily) for a week. If the MAP was more than 108 mm Hg at the end of the week, dosage of the beta-blocker was doubled the following week; when necessary, doubling was repeated to a maximum dose of 640 mg propranolol and 400 mg atenolol daily. Fifty milligrams atenolol had a greater effect than 80 mg propranolol and was as effective as 160 mg propranolol. The dose-response curve flattened off after 160 mg propranolol and 50 mg atenolol daily. The two highest doses of atenolol lowered MAP more than the highest doses of propranolo. Heart rate slowing was the same for both drugs and did not correlate with the fall in blood pressure. PRA was suppressed by all doses of propranolol, whereas atenolol suppressed PRA only at the 2 highest doses, (200 and 400 mg daily). With the lower propranolol doses, the percent MAP change correlated weakly with the percent PRA change (80 mg--r = 0.41, p less than 0.1; 160 mg--r = 0.64, p less than 0.05). Side effects were minimal, and were noted only with 640 mg propranolol; with this exception, the percentage of patients with no complaints rose when placebo was replaced by beta-blockers."}
{"id": "PMID:487689", "title": "Hydrochlorothiazide-induced sympathetic hyperactivity in hypertensive patients.", "content": "Hydrochlorothiazide-induced diuresis and natriuresis is considered to be responsible for the antihypertensive effect of this drug. After short-term treatment there is decreased cardiac output and increased peripheral resistance which we have found to be attended by increased plasma norepinephrine (NE) levels. After longer treatment cardiac output returns to normal and peripheral resistance declines. At this time, plasma NE levels remain elevated, indicating that peripheral resistance reduction is not a consequence of a reduction of the elevated level of sympathetic activity. These results provide a rationale for the combined use of diuretics and drugs which diminish noradrenergic activity in the treatment of hypertension.", "contents": "Hydrochlorothiazide-induced sympathetic hyperactivity in hypertensive patients. Hydrochlorothiazide-induced diuresis and natriuresis is considered to be responsible for the antihypertensive effect of this drug. After short-term treatment there is decreased cardiac output and increased peripheral resistance which we have found to be attended by increased plasma norepinephrine (NE) levels. After longer treatment cardiac output returns to normal and peripheral resistance declines. At this time, plasma NE levels remain elevated, indicating that peripheral resistance reduction is not a consequence of a reduction of the elevated level of sympathetic activity. These results provide a rationale for the combined use of diuretics and drugs which diminish noradrenergic activity in the treatment of hypertension."}
{"id": "PMID:487690", "title": "Effects in normal subjects of long-term administration of azosemide.", "content": "Azosemide is a new loop diuretic which has been shown to affect solute transport proximal to the diluting segment. We assessed the effects of chronic administration of azosemide in normal subjects on low and normal salt diets. In both, there was compensatory renin release and aldosterone secretion, but the subjects on the low sodium diet developed striking hyperuricemia and metabolic alkalosis and were symptomatic, whereas those on the normal diet compensated to the extent that there were only minor changes.", "contents": "Effects in normal subjects of long-term administration of azosemide. Azosemide is a new loop diuretic which has been shown to affect solute transport proximal to the diluting segment. We assessed the effects of chronic administration of azosemide in normal subjects on low and normal salt diets. In both, there was compensatory renin release and aldosterone secretion, but the subjects on the low sodium diet developed striking hyperuricemia and metabolic alkalosis and were symptomatic, whereas those on the normal diet compensated to the extent that there were only minor changes."}
{"id": "PMID:487691", "title": "Haloperidol-induced syndrome of inappropriate secretion of antidiuretic hormone.", "content": "Several psychotropic drugs have been associated with the syndrome of inappropriate secretion of antidiuretic hormone (SIADH). We report here a patient who on two occasions developed clinically significant hyponatremia while receiving the antipsychotic drug, haloperidol. The criteria for the syndrome of inappropriate secretion of ADH were fulfilled and other causes of SIADH were excluded. We documented inability in this patient to excrete a water load while on haloperidol and normal ability in its absence. This case therefore illustrates SIADH due to haloperidol.", "contents": "Haloperidol-induced syndrome of inappropriate secretion of antidiuretic hormone. Several psychotropic drugs have been associated with the syndrome of inappropriate secretion of antidiuretic hormone (SIADH). We report here a patient who on two occasions developed clinically significant hyponatremia while receiving the antipsychotic drug, haloperidol. The criteria for the syndrome of inappropriate secretion of ADH were fulfilled and other causes of SIADH were excluded. We documented inability in this patient to excrete a water load while on haloperidol and normal ability in its absence. This case therefore illustrates SIADH due to haloperidol."}
{"id": "PMID:487686", "title": "Pharmacokinetics of N1-acetyl- and N4-acetylsulphamethoxazole in man.", "content": "The pharmacokinetics of N1-acetylsulphamethoxazole and N4-acetylsulphamethoxazole in man are described. N1-Acetylsulphamethoxazole is deacetylated to sulphamethoxazole and acetylated to N4-acetylsulphamethoxazole. N4-Acetylsulphamethoxazole is excreted almost unchanged in the urine. The renal excretion rate is independent of the urine flow and urinary pH. N4-Acetylsulphonamides are less lipid soluble and more acidic than their corresponding parent sulphonamides.", "contents": "Pharmacokinetics of N1-acetyl- and N4-acetylsulphamethoxazole in man. The pharmacokinetics of N1-acetylsulphamethoxazole and N4-acetylsulphamethoxazole in man are described. N1-Acetylsulphamethoxazole is deacetylated to sulphamethoxazole and acetylated to N4-acetylsulphamethoxazole. N4-Acetylsulphamethoxazole is excreted almost unchanged in the urine. The renal excretion rate is independent of the urine flow and urinary pH. N4-Acetylsulphonamides are less lipid soluble and more acidic than their corresponding parent sulphonamides."}
{"id": "PMID:487692", "title": "Phenytoin cumulation kinetics.", "content": "Four male subjects were given phenytoin orally in single or twice-daily doses. Subjects were on 2 or 3 different dosing rates from 260 to 600 mg phenytoin sodium daily. Predose blood samples were obtained almost daily. The resulting serum levels, measured by gas-liquid chromatography, ranged from 1 to 18 micrograms/ml. Serum phenytoin concentration-time data were fit to a 1-compartment open model with zero-order input and Michaelis-Menten elimination. The resulting computer-generated parameter estimates (Vmax, 5.28 to 8.41 mg/kg/day; Km, 0.83 to 4.18 mg/1; Vd, 0.74 to 0.97 1/kg) are in agreement with the ranges of values in the literature. The time course of phenytoin cumulation is compatible with the presence of a major elimination pathway exhibiting Michaelis-Menten kinetic behavior.", "contents": "Phenytoin cumulation kinetics. Four male subjects were given phenytoin orally in single or twice-daily doses. Subjects were on 2 or 3 different dosing rates from 260 to 600 mg phenytoin sodium daily. Predose blood samples were obtained almost daily. The resulting serum levels, measured by gas-liquid chromatography, ranged from 1 to 18 micrograms/ml. Serum phenytoin concentration-time data were fit to a 1-compartment open model with zero-order input and Michaelis-Menten elimination. The resulting computer-generated parameter estimates (Vmax, 5.28 to 8.41 mg/kg/day; Km, 0.83 to 4.18 mg/1; Vd, 0.74 to 0.97 1/kg) are in agreement with the ranges of values in the literature. The time course of phenytoin cumulation is compatible with the presence of a major elimination pathway exhibiting Michaelis-Menten kinetic behavior."}
{"id": "PMID:487693", "title": "Effects of nefopam on visual tracking.", "content": "The effects of 2 doses of nefopam, d-amphetamine, pentazocine, and placebo were studied in healthy male sleep-deprived volunteers to determine whether the drugs improved or impaired coordination and whether they induced subjective effects. A critical tracking task was used to study hand-eye coordination. D-amphetamine, 10 mg orally, significantly improved tracking performance and made subjects feel better able to perform tasks but more anxious. It also made them feel more alert, steady, sociable, and strong. Pentazocine, 45 mg intramuscularly, caused deterioration in tracking performance and was followed by reports of depression, gloominess, dreaminess, nausea, and injection site pain. There was no significant change in tracking performance or subjective effects after both doses of nefopam and placebo.", "contents": "Effects of nefopam on visual tracking. The effects of 2 doses of nefopam, d-amphetamine, pentazocine, and placebo were studied in healthy male sleep-deprived volunteers to determine whether the drugs improved or impaired coordination and whether they induced subjective effects. A critical tracking task was used to study hand-eye coordination. D-amphetamine, 10 mg orally, significantly improved tracking performance and made subjects feel better able to perform tasks but more anxious. It also made them feel more alert, steady, sociable, and strong. Pentazocine, 45 mg intramuscularly, caused deterioration in tracking performance and was followed by reports of depression, gloominess, dreaminess, nausea, and injection site pain. There was no significant change in tracking performance or subjective effects after both doses of nefopam and placebo."}
{"id": "PMID:487695", "title": "d-Propoxyphene kinetics after single oral and intravenous doses in man.", "content": "d-Propoxyphene kinetics was studied in 8 healthy male subjects after single oral doses of d-propoxyphene at 65, 130, and 190 mg and after slow intravenous infusion of 65 mg. Total urinary excretion (7 days) indicated complete oral absorption but systemic availability was reduced corresponding to fist-pass elimination of 30% to 70%. There was linearity between oral dose and the corresponding area under the plasma concentration/time curve of d-propoxyphene and the metabolite norpropoxyphene. The kinetic measurements showed 2- to 3-fold interindividual variations: oral clearance, 1.3 to 3.6 1/min; systemic clearance, 0.6 to 1.2 1/min; apparent volume of distribution, 700 to 1,800 1; d-propoxyphene half-life (t1/2), 8 to 24 hr; and norpropoxyphene t1/2, 18 to 29 hr. There were pronounced intraindividual dose-dependent variations in oral clearance in some subjects. The intravenous concentration curves indicated a 3-compartment distribution model.", "contents": "d-Propoxyphene kinetics after single oral and intravenous doses in man. d-Propoxyphene kinetics was studied in 8 healthy male subjects after single oral doses of d-propoxyphene at 65, 130, and 190 mg and after slow intravenous infusion of 65 mg. Total urinary excretion (7 days) indicated complete oral absorption but systemic availability was reduced corresponding to fist-pass elimination of 30% to 70%. There was linearity between oral dose and the corresponding area under the plasma concentration/time curve of d-propoxyphene and the metabolite norpropoxyphene. The kinetic measurements showed 2- to 3-fold interindividual variations: oral clearance, 1.3 to 3.6 1/min; systemic clearance, 0.6 to 1.2 1/min; apparent volume of distribution, 700 to 1,800 1; d-propoxyphene half-life (t1/2), 8 to 24 hr; and norpropoxyphene t1/2, 18 to 29 hr. There were pronounced intraindividual dose-dependent variations in oral clearance in some subjects. The intravenous concentration curves indicated a 3-compartment distribution model."}
{"id": "PMID:487696", "title": "Drug metabolism in liver disease: activity of hepatic microsomal metabolizing enzymes.", "content": "The concentration of cytochrome P-450 and activities of the microsomal enzymes aryl hydrocarbon hydroxylase and ethylmorphine demethylase were measured in hepatic tissue obtained at biopsy from 69 patients. Antipyrine half-life (AP t1/2) was measured simultaneously as an in vivo marker of drug metabolism. Values for each index of the drug-metabolizing system varied greatly, but the mean values in groups of patients with mild hepatitis or inactive cirrhosis did not differ significantly from those of controls. Hepatic cytochrome P-450 content and aryl hydrocarbon hydroxylase activity were lower in patients with severe hepatitis or active cirrhosis than in controls, but ethylmorphine demethylase activity was unchanged in the patients. Drug ingestion was associated with enhancement of drug-metabolizing enzymes in all patients but those with severe liver disease; ethylmorphine demethylase activity was enhanced proportionately more than aryl hydrocarbon hydroxylase activity or cytochrome P-450 concentration. The observation that aryl hydrocarbon hydroxylase and ethylmorphine demethylase activities are influenced to a different extent by liver disease and also by drug ingestion indicates functional heterogeneity of the hepatic microsomal drug-metabolizing system in man. Correlations between t1/2 and hepatic drug oxidases were weak, even when allowance was made for variation in liver size. Thus, the rate of drug metabolism in vivo assessed by measuring AP t1/2 does not appear to be closely related to the activity of some hepatic drug-metabolizing enzymes.", "contents": "Drug metabolism in liver disease: activity of hepatic microsomal metabolizing enzymes. The concentration of cytochrome P-450 and activities of the microsomal enzymes aryl hydrocarbon hydroxylase and ethylmorphine demethylase were measured in hepatic tissue obtained at biopsy from 69 patients. Antipyrine half-life (AP t1/2) was measured simultaneously as an in vivo marker of drug metabolism. Values for each index of the drug-metabolizing system varied greatly, but the mean values in groups of patients with mild hepatitis or inactive cirrhosis did not differ significantly from those of controls. Hepatic cytochrome P-450 content and aryl hydrocarbon hydroxylase activity were lower in patients with severe hepatitis or active cirrhosis than in controls, but ethylmorphine demethylase activity was unchanged in the patients. Drug ingestion was associated with enhancement of drug-metabolizing enzymes in all patients but those with severe liver disease; ethylmorphine demethylase activity was enhanced proportionately more than aryl hydrocarbon hydroxylase activity or cytochrome P-450 concentration. The observation that aryl hydrocarbon hydroxylase and ethylmorphine demethylase activities are influenced to a different extent by liver disease and also by drug ingestion indicates functional heterogeneity of the hepatic microsomal drug-metabolizing system in man. Correlations between t1/2 and hepatic drug oxidases were weak, even when allowance was made for variation in liver size. Thus, the rate of drug metabolism in vivo assessed by measuring AP t1/2 does not appear to be closely related to the activity of some hepatic drug-metabolizing enzymes."}
{"id": "PMID:487698", "title": "Tobramycin pharmacokinetics in morbidly obese patients.", "content": "Tobramycin kinetics were examined in 9 morbidly obese women following a single intravenous (120 mg) bolus. After the injection, serum elimination conformed to a 2-compartment open model with alpha and beta t1/2s of 0.285 and 2.1 hr. The volume of distribution (Varea) was determined to be 0.44 1/kg ideal body weight (IBW) and 0.20 1/kg total body weight (TBW). To normalize Varea to 0.26 1/kg, 58% of the patients' adipose weight (TBW -- IBW) must also be taken into account.", "contents": "Tobramycin pharmacokinetics in morbidly obese patients. Tobramycin kinetics were examined in 9 morbidly obese women following a single intravenous (120 mg) bolus. After the injection, serum elimination conformed to a 2-compartment open model with alpha and beta t1/2s of 0.285 and 2.1 hr. The volume of distribution (Varea) was determined to be 0.44 1/kg ideal body weight (IBW) and 0.20 1/kg total body weight (TBW). To normalize Varea to 0.26 1/kg, 58% of the patients' adipose weight (TBW -- IBW) must also be taken into account."}
{"id": "PMID:487699", "title": "Influence of methylxanthine-containing foods on theophylline metabolism and kinetics.", "content": "The metabolism and kinetics of 14C-labeled theophylline have been studied after intravenous doses of 100 mg to healthy subjects, keeping to their usual diets after 7 days of abstention from methylxanthine-containing foods and beverages and again after such abstention while taking caffeine and theophylline in tablet form. The metabolism of oral 14C-theophylline has also been investigated. Metabolites were separated and quantitated by ion-exchange column chromatography, ion-exchange paper chromatography, and liquid scintillation counting. Three major metabolites were found in urine in addition to theophylline, namely 3-methylxanthine, 1,3-dimethyluric acid, and 1-methyluric acid, and 2 minor metabolites were detected but not identified. The elimination kinetics were studied after intravenous administration; theophylline, 1,3-dimethyluric acid, and 1-methyluric acid were eliminated by first-order processes, while elimination of 3-methylxanthine was described by Michaelis-Menten kinetics. Abstention from methylxanthine-containing foods and beverages led to a significant decrease in the urinary elimination half-life of 14C from 9.8 to 7.0 hr (p less than 0.02) due to increases in the elimination constants for theophylline, 3-methylxanthine, and 1,3-dimethyluric acid. When the methylxanthine content of the methylxanthine-containing foods and beverages was replaced by caffeine and theophylline in table form, kinetics and metabolism of theophylline were the same as in subjects on usual diets.", "contents": "Influence of methylxanthine-containing foods on theophylline metabolism and kinetics. The metabolism and kinetics of 14C-labeled theophylline have been studied after intravenous doses of 100 mg to healthy subjects, keeping to their usual diets after 7 days of abstention from methylxanthine-containing foods and beverages and again after such abstention while taking caffeine and theophylline in tablet form. The metabolism of oral 14C-theophylline has also been investigated. Metabolites were separated and quantitated by ion-exchange column chromatography, ion-exchange paper chromatography, and liquid scintillation counting. Three major metabolites were found in urine in addition to theophylline, namely 3-methylxanthine, 1,3-dimethyluric acid, and 1-methyluric acid, and 2 minor metabolites were detected but not identified. The elimination kinetics were studied after intravenous administration; theophylline, 1,3-dimethyluric acid, and 1-methyluric acid were eliminated by first-order processes, while elimination of 3-methylxanthine was described by Michaelis-Menten kinetics. Abstention from methylxanthine-containing foods and beverages led to a significant decrease in the urinary elimination half-life of 14C from 9.8 to 7.0 hr (p less than 0.02) due to increases in the elimination constants for theophylline, 3-methylxanthine, and 1,3-dimethyluric acid. When the methylxanthine content of the methylxanthine-containing foods and beverages was replaced by caffeine and theophylline in table form, kinetics and metabolism of theophylline were the same as in subjects on usual diets."}
{"id": "PMID:487703", "title": "Restoration of selective facial nerve function by the nerve-muscle pedicle technique.", "content": "The nerve-muscle pedicle technique for selective reinnervation of paralyzed facial muscles, when combined with a modified muscle-fascia sling and neurotization from the temporal muscle, has been applied in 26 patients to date. The success rate has been 88 per cent for restoration of tonus and 77 per cent for restoration of voluntary motion as well. This procedure offers several significant advantages over other accepted means of reinnervation in patients in whom the seventh nerve itself is not available as a source for restoration of function. It also may have value as a method for rapid restoration of tonus while other means of reinnervation take effect.", "contents": "Restoration of selective facial nerve function by the nerve-muscle pedicle technique. The nerve-muscle pedicle technique for selective reinnervation of paralyzed facial muscles, when combined with a modified muscle-fascia sling and neurotization from the temporal muscle, has been applied in 26 patients to date. The success rate has been 88 per cent for restoration of tonus and 77 per cent for restoration of voluntary motion as well. This procedure offers several significant advantages over other accepted means of reinnervation in patients in whom the seventh nerve itself is not available as a source for restoration of function. It also may have value as a method for rapid restoration of tonus while other means of reinnervation take effect."}
{"id": "PMID:487705", "title": "Facial nerve grafting: a thirty year retrospective review.", "content": "It is axiomatic that nerve grafting be considered as an integral part of every ablative or traumatic injury involving the extratemporal main trunk and branches of the facial nerve. Our experience with 170 autogenous grafts has established the efficacy of the technique and has proved the high regenerative and adaptive capacities of the facial nerve system.", "contents": "Facial nerve grafting: a thirty year retrospective review. It is axiomatic that nerve grafting be considered as an integral part of every ablative or traumatic injury involving the extratemporal main trunk and branches of the facial nerve. Our experience with 170 autogenous grafts has established the efficacy of the technique and has proved the high regenerative and adaptive capacities of the facial nerve system."}
{"id": "PMID:487710", "title": "Cross-face nerve transplant.", "content": "The two stage principle is one of the most important features of the procedure. It allows starting the operation at a very early stage (one to six months). The donor area in the periphery is very well supplied, therefore as many facial fascicles as necessary can be sacrificed on the healthy side. We have never noticed any functional disturbances. The nerves leading to the buccinator muscle and those which innervate the lateral pull of the mouth are especially suitable. This weakening of the strong pull of the mouth is of great value for symmetry but unfortunately relapse to the original state is common. The selection of the nerve fascicles on the healthy side must be executed in a deep layer below the muscles because all large branches are located here. The end of the sural grafts should positioned far back on the paralyzed side to enable easy anastomoses at the second stage. A face lift incision on the paralyzed side and tightening of the skin are of additional value and provide some support to the elongated muscles. The combination of cross-face nerve transplant with other substitutional methods in which muscles are used for reinnervation is very promising. In our experience physiotherapy is a very important measure. It should be started after the onset of the palsy and continued until restoration of the face is complete. It is usually applied three times a week with exponential current and at a strength of 20 to 60 milliamperes. Each group of muscles receives a 2 to 3 minutes dosage. It is helpful if the patient can use a stimulation apparatus at home daily for short treatments.", "contents": "Cross-face nerve transplant. The two stage principle is one of the most important features of the procedure. It allows starting the operation at a very early stage (one to six months). The donor area in the periphery is very well supplied, therefore as many facial fascicles as necessary can be sacrificed on the healthy side. We have never noticed any functional disturbances. The nerves leading to the buccinator muscle and those which innervate the lateral pull of the mouth are especially suitable. This weakening of the strong pull of the mouth is of great value for symmetry but unfortunately relapse to the original state is common. The selection of the nerve fascicles on the healthy side must be executed in a deep layer below the muscles because all large branches are located here. The end of the sural grafts should positioned far back on the paralyzed side to enable easy anastomoses at the second stage. A face lift incision on the paralyzed side and tightening of the skin are of additional value and provide some support to the elongated muscles. The combination of cross-face nerve transplant with other substitutional methods in which muscles are used for reinnervation is very promising. In our experience physiotherapy is a very important measure. It should be started after the onset of the palsy and continued until restoration of the face is complete. It is usually applied three times a week with exponential current and at a strength of 20 to 60 milliamperes. Each group of muscles receives a 2 to 3 minutes dosage. It is helpful if the patient can use a stimulation apparatus at home daily for short treatments."}
{"id": "PMID:487711", "title": "I. Hypoglossal facial anastomosis: its significance for modern facial surgery. II. Combined approach in extratemporal facial nerve reconstruction.", "content": "The difficulties encountered in extratemporial facial nerve reconstruction after extensive trauma or following resection of parotid tumors often lead to unsatisfactory functional and cosmetic results. So far it has been common practice to use as a \"regenerative nerve,\" the original facial nerve. Using this procedure, however, frequently only a portion of the peripheral branches can be reanastomosed. Above all, the \"autoparalytic syndrome\" develops in this situation owing to undirected outgrowth of axons, and an aberration of the blinking-reflex motoneurons occurs. Experience has shown that the isolated use of a cross-face transplant is not suitable for satisfactory functional regeneration. Depending on the individual anatomic situation, a number of possibilities are therefore recommended for a combined approach using several \"regenerative nerves.\" In this connection, the particular physiologic properties of these nerves have to be taken into account which can help to improve considerably the functional and cosmetic results.", "contents": "I. Hypoglossal facial anastomosis: its significance for modern facial surgery. II. Combined approach in extratemporal facial nerve reconstruction. The difficulties encountered in extratemporial facial nerve reconstruction after extensive trauma or following resection of parotid tumors often lead to unsatisfactory functional and cosmetic results. So far it has been common practice to use as a \"regenerative nerve,\" the original facial nerve. Using this procedure, however, frequently only a portion of the peripheral branches can be reanastomosed. Above all, the \"autoparalytic syndrome\" develops in this situation owing to undirected outgrowth of axons, and an aberration of the blinking-reflex motoneurons occurs. Experience has shown that the isolated use of a cross-face transplant is not suitable for satisfactory functional regeneration. Depending on the individual anatomic situation, a number of possibilities are therefore recommended for a combined approach using several \"regenerative nerves.\" In this connection, the particular physiologic properties of these nerves have to be taken into account which can help to improve considerably the functional and cosmetic results."}
{"id": "PMID:487748", "title": "Program evaluation in a rural community mental health center.", "content": "Can a rural community mental health center with limited resources meet the federal guidelines for program evaluation? This case example shows that a rural center can comply with the guidelines through evaluation activities tailored to its special needs and small size.", "contents": "Program evaluation in a rural community mental health center. Can a rural community mental health center with limited resources meet the federal guidelines for program evaluation? This case example shows that a rural center can comply with the guidelines through evaluation activities tailored to its special needs and small size."}
{"id": "PMID:487749", "title": "Resistance to mental health consultation directed at change in public institutions.", "content": "Mental health consultants need to be aware that institutions in a community are frequently resistant to change. Official agreement that change is necessary may still evoke resistance by those individuals most threatened in the agency. The consultant's awareness of how such resistance is manifest and used permits some educative counterefforts which may be effective. There is a brief review of the literature, and case examples are given of effective and noneffective consultation.", "contents": "Resistance to mental health consultation directed at change in public institutions. Mental health consultants need to be aware that institutions in a community are frequently resistant to change. Official agreement that change is necessary may still evoke resistance by those individuals most threatened in the agency. The consultant's awareness of how such resistance is manifest and used permits some educative counterefforts which may be effective. There is a brief review of the literature, and case examples are given of effective and noneffective consultation."}
{"id": "PMID:487750", "title": "Community mental health centers and the \"seed money\" concept: effects of terminating federal funds.", "content": "What happens to community mental health centers when federal funding ends? Analysis of the funding patterns of a cohort of \"graduate\" community mental health centers indicates that these centers remained fiscally viable subsequent to termination of basic federal grants. However, further analysis revealed two distinct funding patterns within the cohort. One group relied primarily on increased third-party reimbursements to offset the end of basic federal grants. The other sought more state funds and additional federal grants available through the Community Mental Health Center Amendments of 1975. As more centers \"graduate,\" federal \"floor funding\" may be necessary to insure the survival of some of them.", "contents": "Community mental health centers and the \"seed money\" concept: effects of terminating federal funds. What happens to community mental health centers when federal funding ends? Analysis of the funding patterns of a cohort of \"graduate\" community mental health centers indicates that these centers remained fiscally viable subsequent to termination of basic federal grants. However, further analysis revealed two distinct funding patterns within the cohort. One group relied primarily on increased third-party reimbursements to offset the end of basic federal grants. The other sought more state funds and additional federal grants available through the Community Mental Health Center Amendments of 1975. As more centers \"graduate,\" federal \"floor funding\" may be necessary to insure the survival of some of them."}
{"id": "PMID:487751", "title": "Perceptional differences in the supervision of paraprofessional mental health workers.", "content": "Paraprofessional mental health workers and their supervisors completed critical incident questionnaires describing their most and least helpful supervision experiences. A categorical scheme summarizing these critical supervision experiences was developed. A comparative analysis indicated that both the paraprofessionals and supervisors recognized the importance of a supportive supervisory relationship. Paraprofessionals placed more emphasis on their supervisor as a role model, whereas supervisors placed more importance on the paraprofessionals' personal dynamics. The implications of the perceptional differences between paraprofessionals and their supervisors are discussed.", "contents": "Perceptional differences in the supervision of paraprofessional mental health workers. Paraprofessional mental health workers and their supervisors completed critical incident questionnaires describing their most and least helpful supervision experiences. A categorical scheme summarizing these critical supervision experiences was developed. A comparative analysis indicated that both the paraprofessionals and supervisors recognized the importance of a supportive supervisory relationship. Paraprofessionals placed more emphasis on their supervisor as a role model, whereas supervisors placed more importance on the paraprofessionals' personal dynamics. The implications of the perceptional differences between paraprofessionals and their supervisors are discussed."}
{"id": "PMID:487752", "title": "Stimulating equity of services to nonwhites in community mental health centers.", "content": "This paper describes a special date-based approach by the National Institute of Mental Health (NIMH) and the Health, Education and Welfare (HEW) regional offices to improve mental health service utilization nationwide for nonwhites and equalize these services with those available to whites.", "contents": "Stimulating equity of services to nonwhites in community mental health centers. This paper describes a special date-based approach by the National Institute of Mental Health (NIMH) and the Health, Education and Welfare (HEW) regional offices to improve mental health service utilization nationwide for nonwhites and equalize these services with those available to whites."}
{"id": "PMID:487753", "title": "Social support and social adjustment: implications for mental health professionals.", "content": "The general importance of an individual's support network has been recognized in the field of community mental health; yet a more detailed understanding of how a client's available social ties may contribute to his or her adjustment is presently lacking. This study used network analysis to examine differences in the social networks of mental health clients to identify factors associated with positive social adjustment. Subjects were selected from three different types of mental health programs as well as from the general population of Marion County, Oregon. Results generally revealed that subjects from the community sample more often would look to immediate family members for support. Better functioning chronic clients emphasized professional contacts, whereas more poorly adjusted chronic clients would look to friends for support. The results have implications both for understanding the nature of the support available to a client and mobilizing the support resources of the existing network of relationships to aid adjustment to community living.", "contents": "Social support and social adjustment: implications for mental health professionals. The general importance of an individual's support network has been recognized in the field of community mental health; yet a more detailed understanding of how a client's available social ties may contribute to his or her adjustment is presently lacking. This study used network analysis to examine differences in the social networks of mental health clients to identify factors associated with positive social adjustment. Subjects were selected from three different types of mental health programs as well as from the general population of Marion County, Oregon. Results generally revealed that subjects from the community sample more often would look to immediate family members for support. Better functioning chronic clients emphasized professional contacts, whereas more poorly adjusted chronic clients would look to friends for support. The results have implications both for understanding the nature of the support available to a client and mobilizing the support resources of the existing network of relationships to aid adjustment to community living."}
{"id": "PMID:487754", "title": "Social indicators and mental health planning: an empirical case study.", "content": "This paper reports data from a series of studies designed to provide an empirical basis for making judgments regarding the utility and validity of social indicators as a method for assessing the need for mental health services. The following findings are included: (a) The methodological sophistication of the social indicators approach used did not greatly affect the utility of the technique as a means of identifying low- and high-need subareas in a large standard metropolitan statistical area. (b) Correlations between social indicator rankings of tracts/enumeration districts and mental health needs as determined by psychiatric scale scores varied in two different counties. (c) The degree of tract/district socioecological homogeneity appears to account for the diverse correlations. (d) Tract and enumeration district social indicator rankings uniquely account for less than 50% of the explained variance of individual mental health scores when analyzed in a regression equation which includes socioeconomic status as a variable.", "contents": "Social indicators and mental health planning: an empirical case study. This paper reports data from a series of studies designed to provide an empirical basis for making judgments regarding the utility and validity of social indicators as a method for assessing the need for mental health services. The following findings are included: (a) The methodological sophistication of the social indicators approach used did not greatly affect the utility of the technique as a means of identifying low- and high-need subareas in a large standard metropolitan statistical area. (b) Correlations between social indicator rankings of tracts/enumeration districts and mental health needs as determined by psychiatric scale scores varied in two different counties. (c) The degree of tract/district socioecological homogeneity appears to account for the diverse correlations. (d) Tract and enumeration district social indicator rankings uniquely account for less than 50% of the explained variance of individual mental health scores when analyzed in a regression equation which includes socioeconomic status as a variable."}
{"id": "PMID:487777", "title": "A computer program for the analysis of crossover designs.", "content": "We describe a program, CROSS, which we have written to obtain potency estimates and other parameters for bioassay data from assays of crossover design. The program permits testing of all assays for statistical validity and calculates the complete analysis of variance for assays of balanced design. The form of data input and the complete documentation of assay results make this program particularly useful for anyone carrying out crossover assays on a routine basis. The analysis of variance presented is also useful for more general biological or medical situations.", "contents": "A computer program for the analysis of crossover designs. We describe a program, CROSS, which we have written to obtain potency estimates and other parameters for bioassay data from assays of crossover design. The program permits testing of all assays for statistical validity and calculates the complete analysis of variance for assays of balanced design. The form of data input and the complete documentation of assay results make this program particularly useful for anyone carrying out crossover assays on a routine basis. The analysis of variance presented is also useful for more general biological or medical situations."}
{"id": "PMID:487778", "title": "A data analysis microcomputer package (DAMP) for biomedical signals.", "content": "The advent of cheap, powerful microcomputer systems makes the analysis of data via sophisticated techniques available to the personnel who are non-specialists in computing systems. The DAMP package described here is intended for use on personal computers and has therefore been written in BASIC for portability. The analysis techniques are powerful, comprising algorithms to perform sample-data generation, plotting displays, digital data filtering, auto-correlation functions, fast Fourier transforms and autoregressive modelling. The last technique contains a number of options including the display of z-plane plots, frequency response of the model, residual plotting and auto-correlation of the residuals. Illustrative results are shown from psychological mood data and rat locomotor activity. The package is designed both to instruct a user in the techniques of spectral analysis, and also to provide a range of methods for investigating time and frequency behaviour of biomedical data.", "contents": "A data analysis microcomputer package (DAMP) for biomedical signals. The advent of cheap, powerful microcomputer systems makes the analysis of data via sophisticated techniques available to the personnel who are non-specialists in computing systems. The DAMP package described here is intended for use on personal computers and has therefore been written in BASIC for portability. The analysis techniques are powerful, comprising algorithms to perform sample-data generation, plotting displays, digital data filtering, auto-correlation functions, fast Fourier transforms and autoregressive modelling. The last technique contains a number of options including the display of z-plane plots, frequency response of the model, residual plotting and auto-correlation of the residuals. Illustrative results are shown from psychological mood data and rat locomotor activity. The package is designed both to instruct a user in the techniques of spectral analysis, and also to provide a range of methods for investigating time and frequency behaviour of biomedical data."}
{"id": "PMID:487779", "title": "DIADEM--a system for the interactive data acquisition and processing in an analytical laboratory.", "content": "A conversational program for the acquisition of experimental data in a multi-user, multi-instrument computer system is described. It assists the researcher when recording on-time data. Due to the simple structure of the dialogue, no special knowledge of computer handling is required by the experimenter. Whereas the experimental methods are versatile, a uniform concept of the dialogue and the file structure is realized.", "contents": "DIADEM--a system for the interactive data acquisition and processing in an analytical laboratory. A conversational program for the acquisition of experimental data in a multi-user, multi-instrument computer system is described. It assists the researcher when recording on-time data. Due to the simple structure of the dialogue, no special knowledge of computer handling is required by the experimenter. Whereas the experimental methods are versatile, a uniform concept of the dialogue and the file structure is realized."}
{"id": "PMID:487780", "title": "A computer model for hydrodynamic shearing of DNA (Gibb's phenomenon): Part II.", "content": "This paper presents an extension of the distribution proposed during hydrodynamic shearing of DNA. In the Fourier least square approximation of uniform random distribution or a square wave, Gibb's phenomena occur at the boundaries. The same behavior occurs at the boundaries of the DNA strands undergoing hydrodynamic shearing of DNA. This hypothesis is corroborated by results from a computer simulation of hydrodynamic shearing of DNA. The evidence helps confirm the kinetic experiments of Britten and Kohne [4]. Furthermore the results predict that sequence very near the end (0.2 LAMBDA) should be the most intact.", "contents": "A computer model for hydrodynamic shearing of DNA (Gibb's phenomenon): Part II. This paper presents an extension of the distribution proposed during hydrodynamic shearing of DNA. In the Fourier least square approximation of uniform random distribution or a square wave, Gibb's phenomena occur at the boundaries. The same behavior occurs at the boundaries of the DNA strands undergoing hydrodynamic shearing of DNA. This hypothesis is corroborated by results from a computer simulation of hydrodynamic shearing of DNA. The evidence helps confirm the kinetic experiments of Britten and Kohne [4]. Furthermore the results predict that sequence very near the end (0.2 LAMBDA) should be the most intact."}
{"id": "PMID:487781", "title": "A computer program for the analysis of dental arch form using the cubic spline function.", "content": "This paper presents the details and logic of a FORTRAN computer program which fits a cubic interpolatory spline to a set of data points digitized from an exact size photographic reproduction of a dental model. It also measures the length of the arc and computes a set of normals to the curve to be used in evaluating the error in the fit of the spline. The program is used in studies of dental arch form and is useful in evaluating changes in the form of the arches due to orthodontic treatment. The measurement of the arc length provides an adequate assessment of space available in the dental arch, which is of importance to the orthodontist.", "contents": "A computer program for the analysis of dental arch form using the cubic spline function. This paper presents the details and logic of a FORTRAN computer program which fits a cubic interpolatory spline to a set of data points digitized from an exact size photographic reproduction of a dental model. It also measures the length of the arc and computes a set of normals to the curve to be used in evaluating the error in the fit of the spline. The program is used in studies of dental arch form and is useful in evaluating changes in the form of the arches due to orthodontic treatment. The measurement of the arc length provides an adequate assessment of space available in the dental arch, which is of importance to the orthodontist."}
{"id": "PMID:487782", "title": "Computer processing of intracardiac electrograms for conduction studies.", "content": "Computer techniques developed to process intracardiac signals recorded in dogs are presented. The signals under measurement are the auricular and ventricular monophasic action potentials and the His bundle electrogram. Computerized measurement of significant timing parameters on simultaneous recordings of these signals can assess quite precisely changes in the normal conduction scheme of the heart provoked by different experimental protocols. Increased accuracy is mainly due to the objective way of defining wave onsets and the processing power of the system used. Signal recording, signal acquisition, automatic waveform measurements, interactive process and production of end result graphs by computer are all described.", "contents": "Computer processing of intracardiac electrograms for conduction studies. Computer techniques developed to process intracardiac signals recorded in dogs are presented. The signals under measurement are the auricular and ventricular monophasic action potentials and the His bundle electrogram. Computerized measurement of significant timing parameters on simultaneous recordings of these signals can assess quite precisely changes in the normal conduction scheme of the heart provoked by different experimental protocols. Increased accuracy is mainly due to the objective way of defining wave onsets and the processing power of the system used. Signal recording, signal acquisition, automatic waveform measurements, interactive process and production of end result graphs by computer are all described."}
{"id": "PMID:487783", "title": "Data security in information systems by language analysis.", "content": "The paper describes how a language analyser (parser) is used as interface between a user and the functions of an information system. A specific user is at log-on forced into a sublanguage analyser, which only recognizes statements relevant to this specific user. Every attempt to use non-allowed functions is rejected, and the user is guided into his sublanguage. The language analyser is defined completely separately from the semantics of the information system, and it can be automatically generated outgoing from a definition of language functions.", "contents": "Data security in information systems by language analysis. The paper describes how a language analyser (parser) is used as interface between a user and the functions of an information system. A specific user is at log-on forced into a sublanguage analyser, which only recognizes statements relevant to this specific user. Every attempt to use non-allowed functions is rejected, and the user is guided into his sublanguage. The language analyser is defined completely separately from the semantics of the information system, and it can be automatically generated outgoing from a definition of language functions."}
{"id": "PMID:487784", "title": "A user's experience with a standard non-linear regression program (BMDP3R).", "content": "A study was made to test and compare the behavior of a standard non-linear regression program (BMDP3R) in fitting data from six classical least-squares problems. The use of three program control parameters is discussed and four measures of regression failure are utilized to give a quantitative reference of success. Recommendations are given to aid the user of packaged programs in the parameter estimation of non-linear regression models.", "contents": "A user's experience with a standard non-linear regression program (BMDP3R). A study was made to test and compare the behavior of a standard non-linear regression program (BMDP3R) in fitting data from six classical least-squares problems. The use of three program control parameters is discussed and four measures of regression failure are utilized to give a quantitative reference of success. Recommendations are given to aid the user of packaged programs in the parameter estimation of non-linear regression models."}
{"id": "PMID:487785", "title": "A computer program for survival comparisons to a standard population.", "content": "PROPHAZ is a computer program created for the analysis of survival data using the general proportional hazards model. It was designed specifically for the situation in which the underlying hazard function may be estimated from the mortality experience of a large reference population, but may be used for other problems as well. Input for the program includes the variables of interest as well as the information necessary for estimating the hazard function (demographic and mortality data). Regression coefficients for the variables of interest are obtained iteratively using the Newton-Raphson method. Utilizing large sample asymptotic theory, x2 statistics are derived which may be used to test hypotheses of the form C beta = 0. Input format is completely flexible for the variables of interest as well as the mortality data.", "contents": "A computer program for survival comparisons to a standard population. PROPHAZ is a computer program created for the analysis of survival data using the general proportional hazards model. It was designed specifically for the situation in which the underlying hazard function may be estimated from the mortality experience of a large reference population, but may be used for other problems as well. Input for the program includes the variables of interest as well as the information necessary for estimating the hazard function (demographic and mortality data). Regression coefficients for the variables of interest are obtained iteratively using the Newton-Raphson method. Utilizing large sample asymptotic theory, x2 statistics are derived which may be used to test hypotheses of the form C beta = 0. Input format is completely flexible for the variables of interest as well as the mortality data."}
{"id": "PMID:487786", "title": "Computed tomography in the diagnosis of brain stem gliomas.", "content": "Twenty patients with brain stem gliomas were diagnosed by CT. The diagnosis was established on the basis of characteristic appearance, density pattern and mass effect. Differentiation from brain stem metastasis or angioma is frequently possible based on CT findings.", "contents": "Computed tomography in the diagnosis of brain stem gliomas. Twenty patients with brain stem gliomas were diagnosed by CT. The diagnosis was established on the basis of characteristic appearance, density pattern and mass effect. Differentiation from brain stem metastasis or angioma is frequently possible based on CT findings."}
{"id": "PMID:487787", "title": "Giant aneurysm of anterior communicating artery simulating 3rd ventricular tumor.", "content": "This is a case report of an anterior communicating artery aneurysm simulating a 3rd ventricular tumor with obstructive hydrocephalus demonstrated on CT scan. Angiography showed the \"tumor\" to be an aneurysm. We believe that giant aneurysms of the anterior communicating artery should be included in the differential diagnosis of suspected 3rd ventricular tumors along with suprasellar masses as seen on CT scans.", "contents": "Giant aneurysm of anterior communicating artery simulating 3rd ventricular tumor. This is a case report of an anterior communicating artery aneurysm simulating a 3rd ventricular tumor with obstructive hydrocephalus demonstrated on CT scan. Angiography showed the \"tumor\" to be an aneurysm. We believe that giant aneurysms of the anterior communicating artery should be included in the differential diagnosis of suspected 3rd ventricular tumors along with suprasellar masses as seen on CT scans."}
{"id": "PMID:487788", "title": "CT for the follow-up of cranial trauma.", "content": "The author's experience in diagnosing lesions of cerebral trauma with the CT scanner are outlined. The study is divided into acute and chronic lesions. Special attention is drawn to the morphology of subdural and intracerebral haematomas, which is dependent upon their duration.", "contents": "CT for the follow-up of cranial trauma. The author's experience in diagnosing lesions of cerebral trauma with the CT scanner are outlined. The study is divided into acute and chronic lesions. Special attention is drawn to the morphology of subdural and intracerebral haematomas, which is dependent upon their duration."}
{"id": "PMID:487789", "title": "Computed tomographic presentation of granulomatous reaction from intraventricular shunt.", "content": "A case is presented in which the patient was diagnosed as having a large mass lesion arising in the region of the quadrigeminal plate. The patient had been shunted in the past for hydrocephalus. The computed tomographic picture was that of a primary brain neoplasm. At surgery extensive granulation tissue was found. The similarity between the CT presentation of this inflammatory mass and a neoplasm is emphasized.", "contents": "Computed tomographic presentation of granulomatous reaction from intraventricular shunt. A case is presented in which the patient was diagnosed as having a large mass lesion arising in the region of the quadrigeminal plate. The patient had been shunted in the past for hydrocephalus. The computed tomographic picture was that of a primary brain neoplasm. At surgery extensive granulation tissue was found. The similarity between the CT presentation of this inflammatory mass and a neoplasm is emphasized."}
{"id": "PMID:487790", "title": "Evaluation of retroperitoneum with computerized tomography and ultrasonography in patients with testicular tumors.", "content": "Results of CT and US examinations in 24 patients with testicular tumors yielded similar sensitivity and specificity in the diagnosis of enlarged retroperitoneal lymph nodes found at laparatomy. Therefore, the choice of either imaging modality may be influenced by other factors such as presence of abundant bowel gas, post-operative wounds, surgical clips in the abdomen, cachexia or obesity.", "contents": "Evaluation of retroperitoneum with computerized tomography and ultrasonography in patients with testicular tumors. Results of CT and US examinations in 24 patients with testicular tumors yielded similar sensitivity and specificity in the diagnosis of enlarged retroperitoneal lymph nodes found at laparatomy. Therefore, the choice of either imaging modality may be influenced by other factors such as presence of abundant bowel gas, post-operative wounds, surgical clips in the abdomen, cachexia or obesity."}
{"id": "PMID:487791", "title": "Clinical application of computed tomography to thyroid disease.", "content": "CT examinations were carried out in 62 patients with various thyroid disorders, 42 patients with thyroid nodules, 12 with diffuse abnormality and 8 of others. Evaluation was made whether CT could provide more information compared with other techniques. Cystic thyroid nodules could be differentiated from solid nodules with a reasonably high degree of accuracy. Uncalcified thyroid carcinoma was noticed as an ill-defined nodule with irregular wall, grossly the same in density with the muscle in plain CT, and not opacified by contrast material. CT has the ability to define the nature of the thyroid nodules better than other nontraumatic diagnostic modalities.", "contents": "Clinical application of computed tomography to thyroid disease. CT examinations were carried out in 62 patients with various thyroid disorders, 42 patients with thyroid nodules, 12 with diffuse abnormality and 8 of others. Evaluation was made whether CT could provide more information compared with other techniques. Cystic thyroid nodules could be differentiated from solid nodules with a reasonably high degree of accuracy. Uncalcified thyroid carcinoma was noticed as an ill-defined nodule with irregular wall, grossly the same in density with the muscle in plain CT, and not opacified by contrast material. CT has the ability to define the nature of the thyroid nodules better than other nontraumatic diagnostic modalities."}
{"id": "PMID:487792", "title": "Computed tomography of a double inferior vena cava: the \"double cava\" sign.", "content": "Right-sided retroperitoneal adenopathy or right adrenal masses demonstrated by CT in cross sectional display may mimic the CT appearance of the inferior- vena cava. This finding has been recently described as the \"double cava\" sign. The authors report this sign in a patient who actually has a double IVC. \"CT angiography\" with the technique described herein, will determine that the retroperitoneal structures demonstrated by CT are indeed vessels rather than enlarged nodes or adrenal masses.", "contents": "Computed tomography of a double inferior vena cava: the \"double cava\" sign. Right-sided retroperitoneal adenopathy or right adrenal masses demonstrated by CT in cross sectional display may mimic the CT appearance of the inferior- vena cava. This finding has been recently described as the \"double cava\" sign. The authors report this sign in a patient who actually has a double IVC. \"CT angiography\" with the technique described herein, will determine that the retroperitoneal structures demonstrated by CT are indeed vessels rather than enlarged nodes or adrenal masses."}
{"id": "PMID:487793", "title": "Automated scene analysis of CT scans.", "content": "Since the advent of computed tomography, there has been an increasing realization that CT scans contain quantitative as well as qualitative information useful in the diagnostic process. Often however, the use of this information is impeded by the tedious manual outlining of the areas of interest in the scan. To alleviate this problem, we have developed a scene segmentation algorithm which will automatically delineate areas of interest in a CT scan. This procedure uses known information about the expected objects in the scan in conjuction with an algorithm to label those objects. The resultant scene segmentation divides the scan into four anatomical areas: skull, normal brain, high density lesions and CSF. After an area of interest is interactively selected by the clinician, volume, density or other quantitative measures may be computed. Limitations of the algorithm and its clinical applications are discussed.", "contents": "Automated scene analysis of CT scans. Since the advent of computed tomography, there has been an increasing realization that CT scans contain quantitative as well as qualitative information useful in the diagnostic process. Often however, the use of this information is impeded by the tedious manual outlining of the areas of interest in the scan. To alleviate this problem, we have developed a scene segmentation algorithm which will automatically delineate areas of interest in a CT scan. This procedure uses known information about the expected objects in the scan in conjuction with an algorithm to label those objects. The resultant scene segmentation divides the scan into four anatomical areas: skull, normal brain, high density lesions and CSF. After an area of interest is interactively selected by the clinician, volume, density or other quantitative measures may be computed. Limitations of the algorithm and its clinical applications are discussed."}
{"id": "PMID:487794", "title": "Measurements of the partial volume phenomenon.", "content": "The partial volume phenomenon can produce subtle changes in the reconstructed value of the linear attenuation coefficient which may in turn make the detection of an object more difficult. A model for studying the effects of the partial volume phenomenon is presented along with measurements made on the General Electric CT/T whole body scanner. The results of these measurements indicate that small differences in the linear attenuation coefficient may go unnoticed when the video display format is used exclusively to evaluate the CT image. It is suggested that this display format incorporate profiles of the CT numbers to assist the viewer in the evaluation of the CT scans.", "contents": "Measurements of the partial volume phenomenon. The partial volume phenomenon can produce subtle changes in the reconstructed value of the linear attenuation coefficient which may in turn make the detection of an object more difficult. A model for studying the effects of the partial volume phenomenon is presented along with measurements made on the General Electric CT/T whole body scanner. The results of these measurements indicate that small differences in the linear attenuation coefficient may go unnoticed when the video display format is used exclusively to evaluate the CT image. It is suggested that this display format incorporate profiles of the CT numbers to assist the viewer in the evaluation of the CT scans."}
{"id": "PMID:487795", "title": "Preliminary report of a new method of whole body CT-scanning.", "content": "The authors suggest a new approach to CT-body examination derived from their experience in spinal investigations. They introduce a method of gantry tilt in association with inclination of the patient, and call the new mode \"longitudino-axial sectioning\". This method makes possible the simultaneous display of several vertebral levels, which is not possible with conventional CT-scanning. They suggest that by extrapolation, the method may be applied to thoracic and abdominal investigations, as well as to the study of joints. In addition, this technique could be the method of choice whenever urinary and biliary tracts have to be investigated for diagnostic and/or interventional purposes.", "contents": "Preliminary report of a new method of whole body CT-scanning. The authors suggest a new approach to CT-body examination derived from their experience in spinal investigations. They introduce a method of gantry tilt in association with inclination of the patient, and call the new mode \"longitudino-axial sectioning\". This method makes possible the simultaneous display of several vertebral levels, which is not possible with conventional CT-scanning. They suggest that by extrapolation, the method may be applied to thoracic and abdominal investigations, as well as to the study of joints. In addition, this technique could be the method of choice whenever urinary and biliary tracts have to be investigated for diagnostic and/or interventional purposes."}
{"id": "PMID:487805", "title": "Restoration of fertility after non-obstructive vasectomy in the rabbit.", "content": "This study was undertaken for the purpose of comparing reversibility following long-term sperm obstruction with that following long-term sperm diversion. The rabbit served as the animal model. Sperm obstruction was produced by transection and ligation of the vasa deferentia near the vasal ampulla; sperm diversion by transection of vasa and anastomosis of afferent limb with urinary bladder. After one year. vasal continuity was reestablished by end-to-end vasovasostomy. Restoration of fertility was more successful after sperm diversion than after sperm obstruction. It is concluded that vasocystostomy in the rabbit is a safe and effective method of male fertility control with greater probability for successful reversal than conventional vasectomy.", "contents": "Restoration of fertility after non-obstructive vasectomy in the rabbit. This study was undertaken for the purpose of comparing reversibility following long-term sperm obstruction with that following long-term sperm diversion. The rabbit served as the animal model. Sperm obstruction was produced by transection and ligation of the vasa deferentia near the vasal ampulla; sperm diversion by transection of vasa and anastomosis of afferent limb with urinary bladder. After one year. vasal continuity was reestablished by end-to-end vasovasostomy. Restoration of fertility was more successful after sperm diversion than after sperm obstruction. It is concluded that vasocystostomy in the rabbit is a safe and effective method of male fertility control with greater probability for successful reversal than conventional vasectomy."}
{"id": "PMID:487806", "title": "Salpingitis associated with the presence of nonmedicated IUDs.", "content": "Fallopian tube tissue obtained at Pomeroy sterilization of 75 normally cycling women, 75 women 2 to 3 days postpartum, and 75 asymptomatic women who had used a nonmedicated IUD for at least a year was examined histologically. There was an increased frequency of tissue edema and congestion in the postpartum group only. The frequency and extent of acute inflammatory changes in the three groups was not significantly different.", "contents": "Salpingitis associated with the presence of nonmedicated IUDs. Fallopian tube tissue obtained at Pomeroy sterilization of 75 normally cycling women, 75 women 2 to 3 days postpartum, and 75 asymptomatic women who had used a nonmedicated IUD for at least a year was examined histologically. There was an increased frequency of tissue edema and congestion in the postpartum group only. The frequency and extent of acute inflammatory changes in the three groups was not significantly different."}
{"id": "PMID:487808", "title": "A collaborative study of the progesterone intrauterine device (Progestasert). The World Health Organization Task Force on Methods for the Regulation of Implantation.", "content": "A randomised double blind study of a plain T-shaped IUD and an active T-shaped IUD releasing 65 micrograms/day of progesterone, has been completed in four centres. A study of 1320 progesterone-releasing IUD's in parous women for 9660 women months of use significant to 18 months, gave a pregnancy rate of 1.0 +/- 0.4, expulsion of 4.7 +/- 0.6 and removals for pain and bleeding of 6.0 +/- 0.7. A detailed analysis of the menstrual bleeding patterns in these patients gave details of the number and length of bleeding and spotting episodes, count of bleeding days, and count of spotting episodes and days for four one-hundred-day reference periods. While the plain IUD contributed a significant number of intermenstrual spotting and bleeding days, the progesterone-releasing IUD contributed more spotting days.", "contents": "A collaborative study of the progesterone intrauterine device (Progestasert). The World Health Organization Task Force on Methods for the Regulation of Implantation. A randomised double blind study of a plain T-shaped IUD and an active T-shaped IUD releasing 65 micrograms/day of progesterone, has been completed in four centres. A study of 1320 progesterone-releasing IUD's in parous women for 9660 women months of use significant to 18 months, gave a pregnancy rate of 1.0 +/- 0.4, expulsion of 4.7 +/- 0.6 and removals for pain and bleeding of 6.0 +/- 0.7. A detailed analysis of the menstrual bleeding patterns in these patients gave details of the number and length of bleeding and spotting episodes, count of bleeding days, and count of spotting episodes and days for four one-hundred-day reference periods. While the plain IUD contributed a significant number of intermenstrual spotting and bleeding days, the progesterone-releasing IUD contributed more spotting days."}
{"id": "PMID:487809", "title": "Interruption of pregnancy with vaginal suppositories containing 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester.", "content": "A new synthesized Prostaglandin E1 analogue, 16,16-dimethyl-trans-delta 2-Prostaglandin E1 methyl ester, has been shown to be effective in termination of 1st and second trimester pregnancy following vaginal administration. Suppositories, each containing 1 mg Prostaglandin E1 derivative, were administered five times to each of fifty pregnant women of five to twenty gestational weeks at three-hourly intervals. The procedure was clinically effective in 86% of the patients resulting in 56% complete and 30% incomplete abortions. The cervix of all patients was dilated up to 7 mm in diameter at the second insertion of the suppository. Vaginal bleeding and lower abdominal pain not requiring sedation were observed in all patients. Diarrhea (42%), vomiting (6%), and fever (4%) were the most common side effects. The Karyopyknotic Index of the vaginal smear increased significantly (p less than 0.01) twelve hours after the initial insertion. The superficial cells of the cervix gradually degenerated during the termination procedure.", "contents": "Interruption of pregnancy with vaginal suppositories containing 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester. A new synthesized Prostaglandin E1 analogue, 16,16-dimethyl-trans-delta 2-Prostaglandin E1 methyl ester, has been shown to be effective in termination of 1st and second trimester pregnancy following vaginal administration. Suppositories, each containing 1 mg Prostaglandin E1 derivative, were administered five times to each of fifty pregnant women of five to twenty gestational weeks at three-hourly intervals. The procedure was clinically effective in 86% of the patients resulting in 56% complete and 30% incomplete abortions. The cervix of all patients was dilated up to 7 mm in diameter at the second insertion of the suppository. Vaginal bleeding and lower abdominal pain not requiring sedation were observed in all patients. Diarrhea (42%), vomiting (6%), and fever (4%) were the most common side effects. The Karyopyknotic Index of the vaginal smear increased significantly (p less than 0.01) twelve hours after the initial insertion. The superficial cells of the cervix gradually degenerated during the termination procedure."}
{"id": "PMID:487810", "title": "The hormonal and immunological status of vasectomised men.", "content": "The concentration of FSH, LH, testosterone and sperm agglutinating and cytotoxic antibodies have been determined in 87 men at varying time intervals after vasectomy. The results indicated that vasectomy is not associated with statistically significant changes in any of these parameters. Furthermore, no correlation existed between the development of sperm antibodies and the peripheral endocrine status of an individual.", "contents": "The hormonal and immunological status of vasectomised men. The concentration of FSH, LH, testosterone and sperm agglutinating and cytotoxic antibodies have been determined in 87 men at varying time intervals after vasectomy. The results indicated that vasectomy is not associated with statistically significant changes in any of these parameters. Furthermore, no correlation existed between the development of sperm antibodies and the peripheral endocrine status of an individual."}
{"id": "PMID:487811", "title": "Use-effectiveness and client satisfaction in six centers teaching the Billings Ovulation Method.", "content": "Use-effectiveness of the Billings Ovulation Method (OM) is reported for 1139 clients from six centers who were followed for at least 24 months. Method failure rates were 1% when calculated by either Pearl formula or life table analysis. Combined method and user failure rates were 20% when calculated with the Pearl formula, and 16% at 12 months and 23% at 24 months by life table analysis. Pregnancy rates were higher for couples using OM alone, than for couples using OM in combination with additional fertility awareness methods or together with barrier methods. The difference was significant when estimated by the Pearl formula but not when analyzed by the life table. The emotional implications underlying the apparent contradiction between a desire to avoid pregnancy and the deliberate coital use of fertile days are reflected in the differences between method and user failure rates, the high client satisfaction levels reported with \"user failure,\" and 56% continuation rate at 24 months. The will require in-depth exploration.", "contents": "Use-effectiveness and client satisfaction in six centers teaching the Billings Ovulation Method. Use-effectiveness of the Billings Ovulation Method (OM) is reported for 1139 clients from six centers who were followed for at least 24 months. Method failure rates were 1% when calculated by either Pearl formula or life table analysis. Combined method and user failure rates were 20% when calculated with the Pearl formula, and 16% at 12 months and 23% at 24 months by life table analysis. Pregnancy rates were higher for couples using OM alone, than for couples using OM in combination with additional fertility awareness methods or together with barrier methods. The difference was significant when estimated by the Pearl formula but not when analyzed by the life table. The emotional implications underlying the apparent contradiction between a desire to avoid pregnancy and the deliberate coital use of fertile days are reflected in the differences between method and user failure rates, the high client satisfaction levels reported with \"user failure,\" and 56% continuation rate at 24 months. The will require in-depth exploration."}
{"id": "PMID:487812", "title": "Copper allergy and copper IUD.", "content": "Epicutaneous tests with copper, nickel and cobalt were performed in 37 patients using copper IUD who had shown side-effects to the IUD. None of them was allergic to copper; four were allergic to nickel and one to cobalt. Metal allergy was not considered to be the cause of the side-effects in any of the patients.", "contents": "Copper allergy and copper IUD. Epicutaneous tests with copper, nickel and cobalt were performed in 37 patients using copper IUD who had shown side-effects to the IUD. None of them was allergic to copper; four were allergic to nickel and one to cobalt. Metal allergy was not considered to be the cause of the side-effects in any of the patients."}
{"id": "PMID:487813", "title": "Subdermal norethindrone pellets -- a method for contraception?", "content": "The mode of action of compressed pellets containing 85 per cent norethindrone (NET) and 15 per cent cholesterol was studied. Four pellets were inserted subcutaneously, in each of five healthy volunteers and left in place for 200--229 days. The NET content of the pellets varied between 23.9 mg and 25.6 mg; and the cholesterol content between 4.2 mg and 4.5 mg. Plasma levels of NET, estradiol and progesterone were determined by radioimmunoassays. Plasma levels of NET varied mostly between 1--2 ng/ml the first month after insertion. After two months plasma levels of NET ranged between 0.5 ng/ml and 1 ng/ml in all volunteers and there was a gradual decrease of the plasma NET levels throughout treatment. Pronounced day-to-day variations in plasma NET levels were recorded. The release rates of NET was calculated to be between 187 micrograms/day and 243 micrograms/day among the five volunteers. Ovulations occurred in four out of five subjects during treatment. This study indicates that the release of gestagen from four NET pellets was only initially high enough to completely inhibit ovulation and that to accomplish full contraceptive efficacy, a higher dose, i.e. more pellets, would have to be inserted.", "contents": "Subdermal norethindrone pellets -- a method for contraception? The mode of action of compressed pellets containing 85 per cent norethindrone (NET) and 15 per cent cholesterol was studied. Four pellets were inserted subcutaneously, in each of five healthy volunteers and left in place for 200--229 days. The NET content of the pellets varied between 23.9 mg and 25.6 mg; and the cholesterol content between 4.2 mg and 4.5 mg. Plasma levels of NET, estradiol and progesterone were determined by radioimmunoassays. Plasma levels of NET varied mostly between 1--2 ng/ml the first month after insertion. After two months plasma levels of NET ranged between 0.5 ng/ml and 1 ng/ml in all volunteers and there was a gradual decrease of the plasma NET levels throughout treatment. Pronounced day-to-day variations in plasma NET levels were recorded. The release rates of NET was calculated to be between 187 micrograms/day and 243 micrograms/day among the five volunteers. Ovulations occurred in four out of five subjects during treatment. This study indicates that the release of gestagen from four NET pellets was only initially high enough to completely inhibit ovulation and that to accomplish full contraceptive efficacy, a higher dose, i.e. more pellets, would have to be inserted."}
{"id": "PMID:487814", "title": "Use of contraceptives prior to and after conception and exposure to other fetal hazards.", "content": "In a large prospective study, set up to determine whether reproductive outcomes are affected by prior contraceptive use, 34,344 women were recruited at their first antenatal visit and followed until pregnancy termination. This paper summarizes the study methods and describes the demographic differences between subjects who used various contraceptives shortly before conception, and those who experienced contraceptive failures. Thirty percent of the women had used oral contraceptives (OCs) during the 5 months prior to conception and 2.4% had continued using them after their LMP. IUDs had been used by 5.8% of women before conception and 1.1% after. If these rates are also true for the whole population of the U.S., they indicate that approximately 70,000 babies are born each year following pill-failures and 30,000 following failures of IUD's. Exposure to other fetal hazards was common, with 48% of pregnant women drinking alcohol, 28% smoking, and 15% receiving diagnostic X-rays. OC use was more common among smokers and drinkers, and OC failures were significantly associated with the use of anti-epileptics, aspirin, and with exposure to radiation.", "contents": "Use of contraceptives prior to and after conception and exposure to other fetal hazards. In a large prospective study, set up to determine whether reproductive outcomes are affected by prior contraceptive use, 34,344 women were recruited at their first antenatal visit and followed until pregnancy termination. This paper summarizes the study methods and describes the demographic differences between subjects who used various contraceptives shortly before conception, and those who experienced contraceptive failures. Thirty percent of the women had used oral contraceptives (OCs) during the 5 months prior to conception and 2.4% had continued using them after their LMP. IUDs had been used by 5.8% of women before conception and 1.1% after. If these rates are also true for the whole population of the U.S., they indicate that approximately 70,000 babies are born each year following pill-failures and 30,000 following failures of IUD's. Exposure to other fetal hazards was common, with 48% of pregnant women drinking alcohol, 28% smoking, and 15% receiving diagnostic X-rays. OC use was more common among smokers and drinkers, and OC failures were significantly associated with the use of anti-epileptics, aspirin, and with exposure to radiation."}
{"id": "PMID:487815", "title": "Pregnancies associated with sperm concentrations below 10 million/ml in clinical studies of a potential male contraceptive method, monthly depot medroxyprogesterone acetate and testosterone esters.", "content": "A potential male contraceptive approach was evaluated in clinical trials involving monthly injections of depot medroxyprogesterone acetate and either subdermal implants of testosterone propionate or monthly injections of testosterone enanthate. Pregnancies occurred in partners of 9 men with recent sperm counts of 10 million/ml or below. In 5 of the 9 instances, the sperm counts were less than 1 million/ml. It appears that male contraceptive methods involving spermatogenic suppression may require attainment and maintenance of azoospermia. The pregnancy rate cannot be calculated, because the extent of other contraceptive use is uncertain. There were no spontaneous abortions. 6 pregnancies were carried to term, and all progeny were normal, based on physical examination at birth or 3 months after birth.", "contents": "Pregnancies associated with sperm concentrations below 10 million/ml in clinical studies of a potential male contraceptive method, monthly depot medroxyprogesterone acetate and testosterone esters. A potential male contraceptive approach was evaluated in clinical trials involving monthly injections of depot medroxyprogesterone acetate and either subdermal implants of testosterone propionate or monthly injections of testosterone enanthate. Pregnancies occurred in partners of 9 men with recent sperm counts of 10 million/ml or below. In 5 of the 9 instances, the sperm counts were less than 1 million/ml. It appears that male contraceptive methods involving spermatogenic suppression may require attainment and maintenance of azoospermia. The pregnancy rate cannot be calculated, because the extent of other contraceptive use is uncertain. There were no spontaneous abortions. 6 pregnancies were carried to term, and all progeny were normal, based on physical examination at birth or 3 months after birth."}
{"id": "PMID:487816", "title": "Inhibition of implantation by intraluminal administration of concanavalin A in mice.", "content": "Important characteristics of endometrial implantation sites are the changes of polarity and molecular composition of the cellular surface. For this reason the masking of surface carbohydrates could lead to an inhibition of the recognition by the blastocyst of the endometrial implantation site. Considering the impact of lectins on carbohydrates, we decided to utilize the intraluminal administration (5 microliter) of different concentrations of concanavalin A (15-60 microgram) in pregnant female mice in the preimplantation phase. An inhibition of 100% of implantation wqs obtained with concentrations of 30 and 60 microgram of the lectin administered on days 3 and 4 of the pregnancy (P less than 0.001). Less important effects were observed on administering 15 or 20 microgram of the lectin (73 and 87% of inhibition) and on utilizing the different doses on days 1 and 2 of the pregnancy. Thus, we conclude that the egg must recognize certain molecules of the endometrial surface (alpha-D-mannopyranose and alpha-D-glucopyranose) in order to implant and that the making of these sites potentially constitutes a new contraceptive approach.", "contents": "Inhibition of implantation by intraluminal administration of concanavalin A in mice. Important characteristics of endometrial implantation sites are the changes of polarity and molecular composition of the cellular surface. For this reason the masking of surface carbohydrates could lead to an inhibition of the recognition by the blastocyst of the endometrial implantation site. Considering the impact of lectins on carbohydrates, we decided to utilize the intraluminal administration (5 microliter) of different concentrations of concanavalin A (15-60 microgram) in pregnant female mice in the preimplantation phase. An inhibition of 100% of implantation wqs obtained with concentrations of 30 and 60 microgram of the lectin administered on days 3 and 4 of the pregnancy (P less than 0.001). Less important effects were observed on administering 15 or 20 microgram of the lectin (73 and 87% of inhibition) and on utilizing the different doses on days 1 and 2 of the pregnancy. Thus, we conclude that the egg must recognize certain molecules of the endometrial surface (alpha-D-mannopyranose and alpha-D-glucopyranose) in order to implant and that the making of these sites potentially constitutes a new contraceptive approach."}
{"id": "PMID:487817", "title": "Carbohydrate metabolism prospectively studied in women using a low-estrogen oral contraceptive for six months.", "content": "Twenty-four women were prospectively evaluated for their carbohydrate metabolic status before and after six months of using an oral contraceptive containing 0.035 mg ethinyl estradiol and 0.4 mg of norethindrone. At each testing, a 100-gm oral glucose load was administered and blood glucose and plasma insulin values were measured over a three-hour period. Nineteen women with \"normal\" control tests demonstrated a significant decrease in their fasting glucose values and two of these women (10.5%) had a slight deterioration of their glucose curves. There were no other significant glucose or insulin changes. They had no change in weight and there was a significant reduction in their blood pressure during the treatment time. Five women had \"borderline abnormal\" control glucose tests, and four of these (80%) improved during the six months of treatment, and two of them (40%) became \"normal.\" Thus, there is no evidence of an adverse effect of this low-estrogen oral contraceptive on carbohydrate metabolism during six months of therapy.", "contents": "Carbohydrate metabolism prospectively studied in women using a low-estrogen oral contraceptive for six months. Twenty-four women were prospectively evaluated for their carbohydrate metabolic status before and after six months of using an oral contraceptive containing 0.035 mg ethinyl estradiol and 0.4 mg of norethindrone. At each testing, a 100-gm oral glucose load was administered and blood glucose and plasma insulin values were measured over a three-hour period. Nineteen women with \"normal\" control tests demonstrated a significant decrease in their fasting glucose values and two of these women (10.5%) had a slight deterioration of their glucose curves. There were no other significant glucose or insulin changes. They had no change in weight and there was a significant reduction in their blood pressure during the treatment time. Five women had \"borderline abnormal\" control glucose tests, and four of these (80%) improved during the six months of treatment, and two of them (40%) became \"normal.\" Thus, there is no evidence of an adverse effect of this low-estrogen oral contraceptive on carbohydrate metabolism during six months of therapy."}
{"id": "PMID:487818", "title": "First year clinical experience with six levonorgestrel rods as subdermal contraception.", "content": "Previous clinical experience with six levonorgestrel subdermal capsules showed a very good contraceptive effectiveness and continuation rate, but with a high proportion of bleeding disturbances, particularly of increased bleeding. It was hypothesized that bleeding could be reduced by higher plasma levels of the steroid, using subdermal rods instead of capsules, as rods have been shown to have a release rate 3 or 4 times that of the capsules. Fifty volunteers were enrolled in a study of the clinical performance of 6 subdermal levonorgestrel rods. Results were compared with a previous experience with 100 volunteers who wore 6 levonorgestrel capsules for a year in the same clinic. Requirement for volunteers to enter the study were the same for capsules and rods. The proportion of women wearing 6 subdermal rods who had increased bleeding was about one half as for the women using 6 levonorgestrel capsules. On the other hand, women using rods had about twice the incidence of amenhorrea, and about four times more hypomenorrhea (4 days or less of bleeding in a 90-day period). There were no pregnancies among the rod users and the discontinuation rate for medical reasons was not higher than for capsule users in the same clinic.", "contents": "First year clinical experience with six levonorgestrel rods as subdermal contraception. Previous clinical experience with six levonorgestrel subdermal capsules showed a very good contraceptive effectiveness and continuation rate, but with a high proportion of bleeding disturbances, particularly of increased bleeding. It was hypothesized that bleeding could be reduced by higher plasma levels of the steroid, using subdermal rods instead of capsules, as rods have been shown to have a release rate 3 or 4 times that of the capsules. Fifty volunteers were enrolled in a study of the clinical performance of 6 subdermal levonorgestrel rods. Results were compared with a previous experience with 100 volunteers who wore 6 levonorgestrel capsules for a year in the same clinic. Requirement for volunteers to enter the study were the same for capsules and rods. The proportion of women wearing 6 subdermal rods who had increased bleeding was about one half as for the women using 6 levonorgestrel capsules. On the other hand, women using rods had about twice the incidence of amenhorrea, and about four times more hypomenorrhea (4 days or less of bleeding in a 90-day period). There were no pregnancies among the rod users and the discontinuation rate for medical reasons was not higher than for capsule users in the same clinic."}
{"id": "PMID:487819", "title": "Comparison of segmented polyether urethane with polyethylene IUDs in rabbits.", "content": "A segmented polyether urethane IUD was compared with a polyethylene IUD in rabbits. The contraceptive efficacy of urethane IUDs was excellent. Moreover, the purulent slippery deposit present with the polyethylene IUDs and observed by Davis et al. (1) was absent. Our studies indicate that the polyether urethane IUDs have a high degree of antifertility activity in rabbits and these IUDs have reduced inflammatory response, based on leucocytic infiltration and tissue debris in the uterine lumen. It is suggested that polyether urethane IUDs not requiring copper or other medication can be designed for high contraceptive efficacy, intrauterine compatibility and with the necessary rigidity for proper uterine retention in humans.", "contents": "Comparison of segmented polyether urethane with polyethylene IUDs in rabbits. A segmented polyether urethane IUD was compared with a polyethylene IUD in rabbits. The contraceptive efficacy of urethane IUDs was excellent. Moreover, the purulent slippery deposit present with the polyethylene IUDs and observed by Davis et al. (1) was absent. Our studies indicate that the polyether urethane IUDs have a high degree of antifertility activity in rabbits and these IUDs have reduced inflammatory response, based on leucocytic infiltration and tissue debris in the uterine lumen. It is suggested that polyether urethane IUDs not requiring copper or other medication can be designed for high contraceptive efficacy, intrauterine compatibility and with the necessary rigidity for proper uterine retention in humans."}
{"id": "PMID:487820", "title": "The protective influence of progestogen only contraception against vaginal moniliasis.", "content": "The incidence of Candida albicans (C.A.) infection of the vagina was evaluated in vaginal discharges obtained from 85 subjects using a large battery of culture media and confirmatory tests. Twenty women using DMPA 150 mg i.m. contraceptive injections for more than one year (long-term users) were compared with forty 2nd trimester pregnant cases with respect to vaginal candidiasis. The basis of comparison was the presence of amenorrhea and high hormonal levels in both groups. However, pregnant subjects showed a 60% prevalence while none of the DMPA cases had a positive culture or smear. Another group of 25 cases was similarly studied once before DMPA injection and again three months following drug administration (short-term use). The pre-therapy samples demonstrated a 32% incidence which was reduced to 8% after three months of use. It therefore appears that progestogen only contraceptives have the advantage of some protective influence against monilial vaginitis. Moreover, the use of these drugs may carry the beneficial potential of use in resistant cases of monilial vaginitis, either as a single approach or better as a supplementary procedure to known antifungal agents.", "contents": "The protective influence of progestogen only contraception against vaginal moniliasis. The incidence of Candida albicans (C.A.) infection of the vagina was evaluated in vaginal discharges obtained from 85 subjects using a large battery of culture media and confirmatory tests. Twenty women using DMPA 150 mg i.m. contraceptive injections for more than one year (long-term users) were compared with forty 2nd trimester pregnant cases with respect to vaginal candidiasis. The basis of comparison was the presence of amenorrhea and high hormonal levels in both groups. However, pregnant subjects showed a 60% prevalence while none of the DMPA cases had a positive culture or smear. Another group of 25 cases was similarly studied once before DMPA injection and again three months following drug administration (short-term use). The pre-therapy samples demonstrated a 32% incidence which was reduced to 8% after three months of use. It therefore appears that progestogen only contraceptives have the advantage of some protective influence against monilial vaginitis. Moreover, the use of these drugs may carry the beneficial potential of use in resistant cases of monilial vaginitis, either as a single approach or better as a supplementary procedure to known antifungal agents."}
{"id": "PMID:487836", "title": "Reversion of supraventricular tachyarrhythmias by means of atrial stimulation.", "content": "Thirteen patients with different forms of supraventricular tachyarrhythmia were treated by means of right atrial pacing. Reversion of arrhythmia was obtained in nine out of ten patients with atrial tachycardia or flutter. The remaining three patients, with atrial fibrillation, did not respond to the procedure. The effective pacing rate in converting arrhythmia ranged from 110 to 2400/min, however in five cases it was lower than the atrial rate. The duration of pacing at an effective rate ranged from 20 seconds to 5 minutes in 5 cases, while in the remaining patients it lasted 10 minutes. In two patients the original arrhythmia was converted to atrial fibrillation during stimulation, but in one it disappeared with pacing at 110/min, and in the other, reversion to sinus rhythm occurred spontaneously 4 hours later. No complications were observed. It is concluded that atrial pacing may be useful aid to treat paroxysmal atrial tachycardia or flutter, and can be considered as an alternative procedure whenever there is no response to customary medical treatment or when transthoracic direct current cardioversion is potentially dangerous.", "contents": "Reversion of supraventricular tachyarrhythmias by means of atrial stimulation. Thirteen patients with different forms of supraventricular tachyarrhythmia were treated by means of right atrial pacing. Reversion of arrhythmia was obtained in nine out of ten patients with atrial tachycardia or flutter. The remaining three patients, with atrial fibrillation, did not respond to the procedure. The effective pacing rate in converting arrhythmia ranged from 110 to 2400/min, however in five cases it was lower than the atrial rate. The duration of pacing at an effective rate ranged from 20 seconds to 5 minutes in 5 cases, while in the remaining patients it lasted 10 minutes. In two patients the original arrhythmia was converted to atrial fibrillation during stimulation, but in one it disappeared with pacing at 110/min, and in the other, reversion to sinus rhythm occurred spontaneously 4 hours later. No complications were observed. It is concluded that atrial pacing may be useful aid to treat paroxysmal atrial tachycardia or flutter, and can be considered as an alternative procedure whenever there is no response to customary medical treatment or when transthoracic direct current cardioversion is potentially dangerous."}
{"id": "PMID:487837", "title": "Polythelia associated with disturbances of cardiac conduction.", "content": "The authors examined three groups of persons (hospitalized patients, outpatients, and school girls) and conclude that in the cases of polythelia the cardiac conduction disturbances occur considerably more frequently than in persons without polythelia. When polythelia and conduction disturbances occur frequently in a family, the coexistence of both disorders is most probably of hereditary origin.", "contents": "Polythelia associated with disturbances of cardiac conduction. The authors examined three groups of persons (hospitalized patients, outpatients, and school girls) and conclude that in the cases of polythelia the cardiac conduction disturbances occur considerably more frequently than in persons without polythelia. When polythelia and conduction disturbances occur frequently in a family, the coexistence of both disorders is most probably of hereditary origin."}
{"id": "PMID:487838", "title": "Structural rebuilding of pulmonary vessels at various haemodynamic shifts in the lesser circulation.", "content": "The rebuilding of pulmonary vessels at hypo- and hypertension in the lesser circulation was studied in 41 puppy dogs, in which hypotension had been induced by stenosing the pulmonary artery. Eighteen animals were successively sacrificed 6--9 months after the surgery. Histological examinations revealed atrophic and sclerotic lesions of the pulmonary arteries. In the remaining 23 animals, various anastomoses were formed between the systemic and pulmonary circulation systems, which led to the formation of hypertrophic and hyperplastic alterations in the pulmonary vessels and to pulmonary hypertension.", "contents": "Structural rebuilding of pulmonary vessels at various haemodynamic shifts in the lesser circulation. The rebuilding of pulmonary vessels at hypo- and hypertension in the lesser circulation was studied in 41 puppy dogs, in which hypotension had been induced by stenosing the pulmonary artery. Eighteen animals were successively sacrificed 6--9 months after the surgery. Histological examinations revealed atrophic and sclerotic lesions of the pulmonary arteries. In the remaining 23 animals, various anastomoses were formed between the systemic and pulmonary circulation systems, which led to the formation of hypertrophic and hyperplastic alterations in the pulmonary vessels and to pulmonary hypertension."}
{"id": "PMID:487839", "title": "The development of the vascular system in man.", "content": "On the basis of material comprising 10 developmental stages of man in 35 embryonic series, the authors tried to determine the critical developmental period of the blood vessel wall. This period falls between the 5th and 16th weeks of intrauterine life, that is in the period of the formation of the basic wall structure characteristic of the particular vessel type (i. e. the elastic and muscular arteries, large veins, limb veins, and lymphatics). In limbs the differences between the superficial and deep veins as regards the vessel wall development, were disclosed.", "contents": "The development of the vascular system in man. On the basis of material comprising 10 developmental stages of man in 35 embryonic series, the authors tried to determine the critical developmental period of the blood vessel wall. This period falls between the 5th and 16th weeks of intrauterine life, that is in the period of the formation of the basic wall structure characteristic of the particular vessel type (i. e. the elastic and muscular arteries, large veins, limb veins, and lymphatics). In limbs the differences between the superficial and deep veins as regards the vessel wall development, were disclosed."}
{"id": "PMID:487842", "title": "Method of mosaic electrocardiotopography and its practical use in diagnostics of ischaemic heart disease.", "content": "The results of mosaic electrocardiotopographic (MECTG) and coronarographic examinations in 217 men with ichaemic heart diseases (IHD) are compared. On the body surface the zones were identified that corresponded with projections of myocardial electric potentials from individual segments perfused from the main coronary arteries. A distinct correlation was found between the MECTG indicators and the condition of the coronary circulation. The informative value of MECTG in the detection of IHD and determination of the extent of myocardial lesion in individual perfused segments, and the contribution of the method combined with coronarography to the diagnostics of noncoronarogenic myocardial lesions are pointed out.", "contents": "Method of mosaic electrocardiotopography and its practical use in diagnostics of ischaemic heart disease. The results of mosaic electrocardiotopographic (MECTG) and coronarographic examinations in 217 men with ichaemic heart diseases (IHD) are compared. On the body surface the zones were identified that corresponded with projections of myocardial electric potentials from individual segments perfused from the main coronary arteries. A distinct correlation was found between the MECTG indicators and the condition of the coronary circulation. The informative value of MECTG in the detection of IHD and determination of the extent of myocardial lesion in individual perfused segments, and the contribution of the method combined with coronarography to the diagnostics of noncoronarogenic myocardial lesions are pointed out."}
{"id": "PMID:487843", "title": "The hypercoagulability states.", "content": "Although interest in coagulation has usually centered about the various hemorraghic disorders, it is hyperactivity of the hemostatic system with its thrombotic complications which has a far greater clinical impact. The hypercoagulability states are a vague group of disorders not well defined by the laboratory. They fall into two distinct groups: (1) conditions promoting venous thrombosis by activating the coagulation mechanism and (2) conditions promoting arterial thrombosis by platelet plug formation. Dealing with both of these groups separately the various disorders associated with a hypercoagulable state are discussed as well as their pathophysiologic basis; Special emphasis is placed on the laboratory evaluation of these disorders.", "contents": "The hypercoagulability states. Although interest in coagulation has usually centered about the various hemorraghic disorders, it is hyperactivity of the hemostatic system with its thrombotic complications which has a far greater clinical impact. The hypercoagulability states are a vague group of disorders not well defined by the laboratory. They fall into two distinct groups: (1) conditions promoting venous thrombosis by activating the coagulation mechanism and (2) conditions promoting arterial thrombosis by platelet plug formation. Dealing with both of these groups separately the various disorders associated with a hypercoagulable state are discussed as well as their pathophysiologic basis; Special emphasis is placed on the laboratory evaluation of these disorders."}
{"id": "PMID:487845", "title": "Extrapulmonary factors influencing the ratio of arterial oxygen tension to inspired oxygen concentration in burn patients.", "content": "The ratio of arterial oxygen tension to inspired oxygen concentration (PaO2/FIO2) as an index of respiratory function was evaluated in 22 patients with body surface area burns of 15--80%. These results indicate that this ratio is limited in its applicability because extrapulmonary factors, such as cardiac output, oxygen consumption, and arterial oxygen content, can affect this index by alterations in the amount of venous desaturation. Useful estimates of intrapulmonary right to left shunt (Qs/Qt) from PaO2/FIO2 were obtained only when arteriovenous oxygen content differences (avDO2) were between 3--5 ml/dl. There were avDO2 values above and below 3--5 ml/dl in at least 35% of the observations. Under these circumstances, PaO2/FIO2 did not correctly reflect changes in Qs/Qt. Blood gases from central venous catheters did not mirror changes in true mixed venous blood and, thus, can lead to erroneous estimations of Qs/Qt. Rational therapy of reduced arterial oxygen saturation requires measurement of both extra- and intrapulmonary factors contributing to arterial desaturation. Measurement of PaO2/FIO2 alone will not estimate these factors.", "contents": "Extrapulmonary factors influencing the ratio of arterial oxygen tension to inspired oxygen concentration in burn patients. The ratio of arterial oxygen tension to inspired oxygen concentration (PaO2/FIO2) as an index of respiratory function was evaluated in 22 patients with body surface area burns of 15--80%. These results indicate that this ratio is limited in its applicability because extrapulmonary factors, such as cardiac output, oxygen consumption, and arterial oxygen content, can affect this index by alterations in the amount of venous desaturation. Useful estimates of intrapulmonary right to left shunt (Qs/Qt) from PaO2/FIO2 were obtained only when arteriovenous oxygen content differences (avDO2) were between 3--5 ml/dl. There were avDO2 values above and below 3--5 ml/dl in at least 35% of the observations. Under these circumstances, PaO2/FIO2 did not correctly reflect changes in Qs/Qt. Blood gases from central venous catheters did not mirror changes in true mixed venous blood and, thus, can lead to erroneous estimations of Qs/Qt. Rational therapy of reduced arterial oxygen saturation requires measurement of both extra- and intrapulmonary factors contributing to arterial desaturation. Measurement of PaO2/FIO2 alone will not estimate these factors."}
{"id": "PMID:487846", "title": "Hypoxemia during hemodialysis.", "content": "Five mechanically ventilated patients were studied during hemodialysis. The aim was to determine if hypoxemia would develop, and to identify the causes. Respiratory variables (dynamic compliance, peak airway pressure, CO2production); oxygen uptake, and transport variables (alveolar and arterial PO2, pulmonary venous admixture, oxygen consumption); respiratory quotient; pulmonary vascular resistances and white blood cells (WBC) were measured. PaO2 decreased during dialysis, as did PaO2. However, the fall in alveolar oxygen tension failed to explain the hypoxemia. Lung volume did not change significantly, because dynamic compliance, peak airway pressure, and pulmonary vascular resistance were not modified. CO2 losses through the dialysis coil were of little clinical significance. WBC count fell significantly. The authors conclude that ventilation/perfusion and diffusion abnormalities related to leuko-agglutination are responsible for hypoxemia during dialysis.", "contents": "Hypoxemia during hemodialysis. Five mechanically ventilated patients were studied during hemodialysis. The aim was to determine if hypoxemia would develop, and to identify the causes. Respiratory variables (dynamic compliance, peak airway pressure, CO2production); oxygen uptake, and transport variables (alveolar and arterial PO2, pulmonary venous admixture, oxygen consumption); respiratory quotient; pulmonary vascular resistances and white blood cells (WBC) were measured. PaO2 decreased during dialysis, as did PaO2. However, the fall in alveolar oxygen tension failed to explain the hypoxemia. Lung volume did not change significantly, because dynamic compliance, peak airway pressure, and pulmonary vascular resistance were not modified. CO2 losses through the dialysis coil were of little clinical significance. WBC count fell significantly. The authors conclude that ventilation/perfusion and diffusion abnormalities related to leuko-agglutination are responsible for hypoxemia during dialysis."}
{"id": "PMID:487869", "title": "The so-called ectopic pinealomas. A cooperative study of 15 cases.", "content": "We present the results of a cooperative study of 15 cases of suprasellar dysgerminomas (ectopic pinealomas) and a review of cases of the literature with histological confirmation. In all the cases, histological similarities to the germinomas which appear extracerebrally, particularly testicular seminomas, were observed. We were unable to find in the sellar region tumors of true pineal histology and tend therefore to confirm the germinal origin of these neoplasms. From a clinical point of view, we define a syndrome that these tumors produce, consisting of diabetes insipidus as the first sign, visual impairment and endocrine disturbances, with few or no radiological signs in plain studies.", "contents": "The so-called ectopic pinealomas. A cooperative study of 15 cases. We present the results of a cooperative study of 15 cases of suprasellar dysgerminomas (ectopic pinealomas) and a review of cases of the literature with histological confirmation. In all the cases, histological similarities to the germinomas which appear extracerebrally, particularly testicular seminomas, were observed. We were unable to find in the sellar region tumors of true pineal histology and tend therefore to confirm the germinal origin of these neoplasms. From a clinical point of view, we define a syndrome that these tumors produce, consisting of diabetes insipidus as the first sign, visual impairment and endocrine disturbances, with few or no radiological signs in plain studies."}
{"id": "PMID:487870", "title": "Congenital vascular malformation of spinal cord simulating diastematomyelia.", "content": "Diastematomyelia is usually caused by a midline bony, cartilaginous or fibrous spur. A case is presented with splitting of the spinal cord by a large arteriovenous malformation at the thoracolumbar junction. A hairy skin patch, kyphoscoliosis, vertebral anomalies and an arachnoidal cyst were found adjacent to the vascular and neural malformations.", "contents": "Congenital vascular malformation of spinal cord simulating diastematomyelia. Diastematomyelia is usually caused by a midline bony, cartilaginous or fibrous spur. A case is presented with splitting of the spinal cord by a large arteriovenous malformation at the thoracolumbar junction. A hairy skin patch, kyphoscoliosis, vertebral anomalies and an arachnoidal cyst were found adjacent to the vascular and neural malformations."}
{"id": "PMID:487871", "title": "Intracranial tumefactions in children. Surgical mortality.", "content": "This paper summarizes a 26.5-year experience in one centre with intracranial tumefactions in children. In 337 craniotomies done on 285 patients, the death rate within 30 days of operation was 6.7% for cases and 5.6% for procedures. Since 1965, the case and operative mortalities have been 2.0 and 1,7%, respectively. There have been no operative deaths in the last 109 patients, 124 craniotomies, done during the past 6.5 years. Operative mortality for primary procedures was 6.0%, 1.5% since 1965. For patients operated upon more than once (52 times on 38 patients), the case mortality has been 5.3%, procedural mortality 3.8%; since 1965 these figures have been 3.8 and 2.8%, respectively. The operative deaths are analysed, and some conclusions drawn. At the present time, surgical mortality from intracranial tumefactions in children should be less than 5%.", "contents": "Intracranial tumefactions in children. Surgical mortality. This paper summarizes a 26.5-year experience in one centre with intracranial tumefactions in children. In 337 craniotomies done on 285 patients, the death rate within 30 days of operation was 6.7% for cases and 5.6% for procedures. Since 1965, the case and operative mortalities have been 2.0 and 1,7%, respectively. There have been no operative deaths in the last 109 patients, 124 craniotomies, done during the past 6.5 years. Operative mortality for primary procedures was 6.0%, 1.5% since 1965. For patients operated upon more than once (52 times on 38 patients), the case mortality has been 5.3%, procedural mortality 3.8%; since 1965 these figures have been 3.8 and 2.8%, respectively. The operative deaths are analysed, and some conclusions drawn. At the present time, surgical mortality from intracranial tumefactions in children should be less than 5%."}
{"id": "PMID:487872", "title": "A long-term postoperative follow-up in craniopharyngioma.", "content": "The long-term postoperative follow-up of children treated for craniopharyngioma is influenced by the patient's age at time of diagnosis, tumor location, texture, interface and adherence to brain, and achievements of the first operation. These factors will be examined as they apply to 51 children treated at the Hospital for Sick Children, Toronto. A brief comment on the role of irradiation treatment is also provided.", "contents": "A long-term postoperative follow-up in craniopharyngioma. The long-term postoperative follow-up of children treated for craniopharyngioma is influenced by the patient's age at time of diagnosis, tumor location, texture, interface and adherence to brain, and achievements of the first operation. These factors will be examined as they apply to 51 children treated at the Hospital for Sick Children, Toronto. A brief comment on the role of irradiation treatment is also provided."}
{"id": "PMID:487873", "title": "Cerebellar abscess in early infancy.", "content": "The authors report an unusual case of cerebellar abscess in a 6-week-old infant without an antecedent of sepsis or congenital heart disease. The abscess was treated successfully by percutaneous punction-aspiration and further excision. Shunting procedure was not necessary.", "contents": "Cerebellar abscess in early infancy. The authors report an unusual case of cerebellar abscess in a 6-week-old infant without an antecedent of sepsis or congenital heart disease. The abscess was treated successfully by percutaneous punction-aspiration and further excision. Shunting procedure was not necessary."}
{"id": "PMID:487875", "title": "Social interactions of young abused children: approach, avoidance, and aggression.", "content": "10 abused toddlers (ages 1-3 years) and 10 matched controls from families experiencing stress were observed during social interactions with caregivers and with peers in their daycare settings. The abused infants more frequently physically assaulted their peers. They \"harassed\" their caregivers verbally and nonverbally, and they were the only infants who assaulted or threatened to assault them. The abused infants were much less likely than the controls to approach their caregivers in response to friendly overtures; when they did so they were more likely to approach to the side, to the rear, or by turning about and backstepping. In response to friendly overtures the abused infants more frequently avoided peers and caregivers or combined movements of approach with movements of avoidance. A similar behavior pattern has been identified by Main in maternally rejected infants in normal samples.", "contents": "Social interactions of young abused children: approach, avoidance, and aggression. 10 abused toddlers (ages 1-3 years) and 10 matched controls from families experiencing stress were observed during social interactions with caregivers and with peers in their daycare settings. The abused infants more frequently physically assaulted their peers. They \"harassed\" their caregivers verbally and nonverbally, and they were the only infants who assaulted or threatened to assault them. The abused infants were much less likely than the controls to approach their caregivers in response to friendly overtures; when they did so they were more likely to approach to the side, to the rear, or by turning about and backstepping. In response to friendly overtures the abused infants more frequently avoided peers and caregivers or combined movements of approach with movements of avoidance. A similar behavior pattern has been identified by Main in maternally rejected infants in normal samples."}
{"id": "PMID:487876", "title": "Child rearing and children's prosocial initiations toward victims of distress.", "content": "Maternal rearing behavior was examined in relation to children's reparation for transgressions and altruism as bystanders to distress in others. The children were 1 1/2-2 1/2 years old. Mothers were trained in techniques of observing. They recorded their child's reactions and their own behaviors in everyday encounters with expressions of distress in others (sorrow, discomfort, pain). Distress was also simulated by mothers and investigators. Mothers' empathic caregiving was rated during home visits. Mothers' affectively delivered explanations regarding the distresses their children had caused to others were associated with children's reparations for transgressions. Such explanations were also associated with children's altruism when they were bystanders to another's distress. Empathic caregiving by mothers was positively associated with children's reparation and altruism. Findings are discussed in relation to theories of altruism, conscience, and child rearing.", "contents": "Child rearing and children's prosocial initiations toward victims of distress. Maternal rearing behavior was examined in relation to children's reparation for transgressions and altruism as bystanders to distress in others. The children were 1 1/2-2 1/2 years old. Mothers were trained in techniques of observing. They recorded their child's reactions and their own behaviors in everyday encounters with expressions of distress in others (sorrow, discomfort, pain). Distress was also simulated by mothers and investigators. Mothers' empathic caregiving was rated during home visits. Mothers' affectively delivered explanations regarding the distresses their children had caused to others were associated with children's reparations for transgressions. Such explanations were also associated with children's altruism when they were bystanders to another's distress. Empathic caregiving by mothers was positively associated with children's reparation and altruism. Findings are discussed in relation to theories of altruism, conscience, and child rearing."}
{"id": "PMID:487877", "title": "Effects of infant sociability and the caretaking environment on infant cognitive performance.", "content": "40 middle-class mothers and their 12-month-old infants participated in an examination of the extent to which infant sociability and infant home experiences were correlated with cognitive capacity. Results indicated that the measures of the home environment (including Caldwell's Home Observation for Measurement of the Environment [HOME] inventory) were not correlated with the measures of cognitive competence (Bayley Mental Development Index [MDI], Ordinal Scales of Psychological Development) except among firstborns. Measures of the home environment were, however, correlated with measures of infant sociability (assessed inside and outside the test situation): sociable infants had sociable mothers. The infants' reactions to strange adults clearly influenced their performance in testing situations. Strong relationships were found between both measures of sociability and both measures of cognitive competence. The sociable friendly infants received higher scores on both cognitive tests than the less sociable babies did.", "contents": "Effects of infant sociability and the caretaking environment on infant cognitive performance. 40 middle-class mothers and their 12-month-old infants participated in an examination of the extent to which infant sociability and infant home experiences were correlated with cognitive capacity. Results indicated that the measures of the home environment (including Caldwell's Home Observation for Measurement of the Environment [HOME] inventory) were not correlated with the measures of cognitive competence (Bayley Mental Development Index [MDI], Ordinal Scales of Psychological Development) except among firstborns. Measures of the home environment were, however, correlated with measures of infant sociability (assessed inside and outside the test situation): sociable infants had sociable mothers. The infants' reactions to strange adults clearly influenced their performance in testing situations. Strong relationships were found between both measures of sociability and both measures of cognitive competence. The sociable friendly infants received higher scores on both cognitive tests than the less sociable babies did."}
{"id": "PMID:487878", "title": "Sex differences in children's expression and control of fantasy and overt aggression.", "content": "The present study explored sex differences in children's expression and control of fantasy and overt aggression. Fifth-grade boys and girls were presented a TAT-like projective test to measure fantasy aggression and controls over aggression. Overt peer-oriented aggression was measured by peer and teacher ratings. Results indicated that boys were rated more physically and verbally aggressive than girls but not more indirectly aggressive. Boys also produced more physical aggression in fantasy than girls, although the opposite trend was found for indirect aggression; no sex difference was found for verbal fantasy aggression. Finally, girls had a higher ratio of aggression control per total fantasy aggression than boys. The results support the position that while boys, in contrast to girls, are socialized in a way that encourage direct expression of aggression, girls are just as likely to be aggressive as boys when the aggression is indirect. The results are discussed further from the perspective of social learning theory and Maccoby and Jacklin's biological position.", "contents": "Sex differences in children's expression and control of fantasy and overt aggression. The present study explored sex differences in children's expression and control of fantasy and overt aggression. Fifth-grade boys and girls were presented a TAT-like projective test to measure fantasy aggression and controls over aggression. Overt peer-oriented aggression was measured by peer and teacher ratings. Results indicated that boys were rated more physically and verbally aggressive than girls but not more indirectly aggressive. Boys also produced more physical aggression in fantasy than girls, although the opposite trend was found for indirect aggression; no sex difference was found for verbal fantasy aggression. Finally, girls had a higher ratio of aggression control per total fantasy aggression than boys. The results support the position that while boys, in contrast to girls, are socialized in a way that encourage direct expression of aggression, girls are just as likely to be aggressive as boys when the aggression is indirect. The results are discussed further from the perspective of social learning theory and Maccoby and Jacklin's biological position."}
{"id": "PMID:487879", "title": "The relationship between quality of infant-mother attachment and infant competence in initial encounters with peers.", "content": "18-month-old infants and their mothers were observed in the Ainsworth strange situation (SS) in order that security of attachment might be assessed. Infant dyads were created for observation in unstructured peer interaction according to their SS classification (security of attachment). Focus was on the subgroups within the securely attached (B) category. Results indicated a relationship between quality of infant-mother attachment and infant peer competence. The B1 and B2 infants engaged in more frequent and more sophisticated interaction with peers than did the B3 and B4 infants, who intensely sought proximity and contact with their mothers in the peer session just as in the SS. The B1 and B2 infants engaged in more distal interaction with their mothers and were more sociable with the peers' mothers and with the stranger in the SS. Implications of individual differences in quality of attachment for the development of social competence and social relationships are discussed.", "contents": "The relationship between quality of infant-mother attachment and infant competence in initial encounters with peers. 18-month-old infants and their mothers were observed in the Ainsworth strange situation (SS) in order that security of attachment might be assessed. Infant dyads were created for observation in unstructured peer interaction according to their SS classification (security of attachment). Focus was on the subgroups within the securely attached (B) category. Results indicated a relationship between quality of infant-mother attachment and infant peer competence. The B1 and B2 infants engaged in more frequent and more sophisticated interaction with peers than did the B3 and B4 infants, who intensely sought proximity and contact with their mothers in the peer session just as in the SS. The B1 and B2 infants engaged in more distal interaction with their mothers and were more sociable with the peers' mothers and with the stranger in the SS. Implications of individual differences in quality of attachment for the development of social competence and social relationships are discussed."}
{"id": "PMID:487880", "title": "Peer interaction in a structured communication task: comparisons of normal and hyperactive boys and of methylphenidate (Ritalin) and placebo effects.", "content": "Peer communication patterns were assessed as school-aged boys participated in a dyadic referential communication task. The responses of comparison boys were compared to those of hyperactive boys on methylphenidate (Ritalin) and on placebo in a double-blind crossover design. 2 separate systems for assessing communication were developed, a qualitative system designed to capture the \"flavor\" of interaction and a quantitative system focused on specific types of communicative content. Task products and completion times were also scored. The results suggest that hyperactive children, regardless of medication status, are less likely than comparison peers to (a) modulate ongoing or habitual behavior patterns in response to externally imposed shifts in role-appropriate behaviors; (b) maintain consistent, uninterrupted goal orientation; and (c) respond to subtle social learning opportunities. In this situation, methylphenidate appeared to have a greater impact on behavioral style than on competence, decreasing perceived intensity without influencing efficiency. A mild medication-induced dysphoria was also documented. Directions for future research and the need for caution in clinical interpretation are discussed.", "contents": "Peer interaction in a structured communication task: comparisons of normal and hyperactive boys and of methylphenidate (Ritalin) and placebo effects. Peer communication patterns were assessed as school-aged boys participated in a dyadic referential communication task. The responses of comparison boys were compared to those of hyperactive boys on methylphenidate (Ritalin) and on placebo in a double-blind crossover design. 2 separate systems for assessing communication were developed, a qualitative system designed to capture the \"flavor\" of interaction and a quantitative system focused on specific types of communicative content. Task products and completion times were also scored. The results suggest that hyperactive children, regardless of medication status, are less likely than comparison peers to (a) modulate ongoing or habitual behavior patterns in response to externally imposed shifts in role-appropriate behaviors; (b) maintain consistent, uninterrupted goal orientation; and (c) respond to subtle social learning opportunities. In this situation, methylphenidate appeared to have a greater impact on behavioral style than on competence, decreasing perceived intensity without influencing efficiency. A mild medication-induced dysphoria was also documented. Directions for future research and the need for caution in clinical interpretation are discussed."}
{"id": "PMID:487881", "title": "Concept acquisition in the human infant.", "content": "The ability of 18-, 24-, and 30-week-old infants to learn conceptual categories regarding adult female faces was examined using a habituation paradigm. Little evidence for conceptual categorization occurred at 18 or 24 weeks, but at 30 weeks infants learned to respond to \"a specific female face regardless of orientation\" and to \"female faces in general.\"", "contents": "Concept acquisition in the human infant. The ability of 18-, 24-, and 30-week-old infants to learn conceptual categories regarding adult female faces was examined using a habituation paradigm. Little evidence for conceptual categorization occurred at 18 or 24 weeks, but at 30 weeks infants learned to respond to \"a specific female face regardless of orientation\" and to \"female faces in general.\""}
{"id": "PMID:487882", "title": "Matching behavior in the young infant.", "content": "Infants as young as 2--6 weeks have been reported to exhibit matching behavior in response to seeing an adult model tongue protrusion and certain other acts. Matching behavior to the tongue model declines by 12 weeks. The present study was designed to investigate whether (1) this matching behavior represents selective imitation or a released response that can be elicited by a broad but delimited class of incentive stimuli and (2) stimulation of tongue protrusion enhances the response and delays its decline. 24 infants were observed at 6, 10, and 14 weeks. A moving pen and ball were as effective as the tongue model in eliciting tongue protrusion at 6 weeks, while a dangling ring elicited as much hand opening and closing as the hand model at 14 weeks. After the 6-week visit, 12 of the infants were exposed to the tongue model daily. This intervention delayed the decline of matching behavior to the tongue model at 14 weeks. Also, experimental infants responded selectively to the tongue model, while the pen continued to be an effective releaser of tongue protrusions among controls.", "contents": "Matching behavior in the young infant. Infants as young as 2--6 weeks have been reported to exhibit matching behavior in response to seeing an adult model tongue protrusion and certain other acts. Matching behavior to the tongue model declines by 12 weeks. The present study was designed to investigate whether (1) this matching behavior represents selective imitation or a released response that can be elicited by a broad but delimited class of incentive stimuli and (2) stimulation of tongue protrusion enhances the response and delays its decline. 24 infants were observed at 6, 10, and 14 weeks. A moving pen and ball were as effective as the tongue model in eliciting tongue protrusion at 6 weeks, while a dangling ring elicited as much hand opening and closing as the hand model at 14 weeks. After the 6-week visit, 12 of the infants were exposed to the tongue model daily. This intervention delayed the decline of matching behavior to the tongue model at 14 weeks. Also, experimental infants responded selectively to the tongue model, while the pen continued to be an effective releaser of tongue protrusions among controls."}
{"id": "PMID:487883", "title": "Newborn infants orient to sounds.", "content": "In 2 experiments, the majority of 21 newborn infants who were maintained in an alert state consistently turned their heads toward a continuous sound source presented 90 degrees from midline. For most infants this orientation response was rather slow, taking median latencies of 2.5 sec to begin and 5.5 sec to end. The most important factors in producing this impressive response seem to be the method of holding the infants during testing and the nature of the auditory stimulus.", "contents": "Newborn infants orient to sounds. In 2 experiments, the majority of 21 newborn infants who were maintained in an alert state consistently turned their heads toward a continuous sound source presented 90 degrees from midline. For most infants this orientation response was rather slow, taking median latencies of 2.5 sec to begin and 5.5 sec to end. The most important factors in producing this impressive response seem to be the method of holding the infants during testing and the nature of the auditory stimulus."}
{"id": "PMID:487884", "title": "Crossing the midline: limits of early eye-hand behavior.", "content": "The development of the ability to extend the hand across the body midline to contact a visually presented object was examined in 48 normal, full-term, 9--20-week infants. One of the infant's arms was restrained while the behavior of the contralateral, unrestrained arm was observed. Results indicate that infants can first contact objects placed in front of the ipsilateral shoulder, then at the body midline, and later in front of the contralateral shoulder. Between 9 and 17 weeks, success at contacting objects at the midline progressed from 33% to 93%. During this interval, the success in contacting objects presented in front of the contralateral shoulder increased from 0% to 71%. By 18--20 weeks, all infants contacted objects in all three positions. These findings indicate that visually directed hand extension and reaching skills progress from the ipsilateral to include the bilateral and later the contralateral domains. The results are considered in regard to the development of bilateral coordination and complementarity.", "contents": "Crossing the midline: limits of early eye-hand behavior. The development of the ability to extend the hand across the body midline to contact a visually presented object was examined in 48 normal, full-term, 9--20-week infants. One of the infant's arms was restrained while the behavior of the contralateral, unrestrained arm was observed. Results indicate that infants can first contact objects placed in front of the ipsilateral shoulder, then at the body midline, and later in front of the contralateral shoulder. Between 9 and 17 weeks, success at contacting objects at the midline progressed from 33% to 93%. During this interval, the success in contacting objects presented in front of the contralateral shoulder increased from 0% to 71%. By 18--20 weeks, all infants contacted objects in all three positions. These findings indicate that visually directed hand extension and reaching skills progress from the ipsilateral to include the bilateral and later the contralateral domains. The results are considered in regard to the development of bilateral coordination and complementarity."}
{"id": "PMID:487885", "title": "Smooth-pursuit eye movements in the newborn infant.", "content": "The capacity of newborn infants to smooth-pursuit eye movements in single-target tracking and in optokinetic nystagmus to a moving striped field was examined utilizing DC electrooculography. Smooth-pursuit movements were observed in all infants who were alert during testing, but they occupied less than 15% of viewing time during single-target tracking. The velocity of smooth-pursuit segments in single-target tracking increased linearly to a target velocity of 19 degrees/sec and deteriorated rapidly at faster speeds. No smooth pursuit occurred above 32 degrees/sec in single-target tracking. By contrast, optokinetic nystagmus was observed at all field velocities to 40 degrees/sec, but the speed of the mean slow component increased linearly to 25 degrees/sec and diminished above that field velocity. Infant optokinetic nystagmus differed strikingly from that of adults in that tonic ocular deviation was in the direction of field movement rather than away from it. This feature of neonatal optokinetic nystagmus is consistent with deficient foveal function and suggests that the relatively poor smooth-pursuit performance of newborns may be related to foveal immaturity.", "contents": "Smooth-pursuit eye movements in the newborn infant. The capacity of newborn infants to smooth-pursuit eye movements in single-target tracking and in optokinetic nystagmus to a moving striped field was examined utilizing DC electrooculography. Smooth-pursuit movements were observed in all infants who were alert during testing, but they occupied less than 15% of viewing time during single-target tracking. The velocity of smooth-pursuit segments in single-target tracking increased linearly to a target velocity of 19 degrees/sec and deteriorated rapidly at faster speeds. No smooth pursuit occurred above 32 degrees/sec in single-target tracking. By contrast, optokinetic nystagmus was observed at all field velocities to 40 degrees/sec, but the speed of the mean slow component increased linearly to 25 degrees/sec and diminished above that field velocity. Infant optokinetic nystagmus differed strikingly from that of adults in that tonic ocular deviation was in the direction of field movement rather than away from it. This feature of neonatal optokinetic nystagmus is consistent with deficient foveal function and suggests that the relatively poor smooth-pursuit performance of newborns may be related to foveal immaturity."}
{"id": "PMID:487886", "title": "Interference of time-related and unrelated cues with duration comparisons of young children: analysis of Piaget's formulation of the relation of time and speed.", "content": "The analysis of the relation of time and speed led Piaget to conclude that the time-speed confusion characterizing the intuitive stage undergoes development. He claimed that in the first stage, a child comparing the duration of two moving bodies attributes longer duration to the faster body, whereas in the second stage, he attributes longer duration to the slower body. It is proposed here that time-speed confusion is mediated simply by \"more is more,\" and hence, as long as the child continues to confuse time with speed, he believes \"faster\" implies longer duration. Moreover, this confusion should not differ either in form or extent from the confusion of time and many other arbitrary quantitative dimensions (e.g., intensity of light). 72 children, 4-7 years old, compared duration differing either on speed or on intensity of light. As predicted, the tasks did not differ on performance level or on error types. Moreover, the performance on both tasks was consistent with the predictions of a facet model pertaining to analysis of time problems. Confusion dissipated with age and was similar for boys and girls.", "contents": "Interference of time-related and unrelated cues with duration comparisons of young children: analysis of Piaget's formulation of the relation of time and speed. The analysis of the relation of time and speed led Piaget to conclude that the time-speed confusion characterizing the intuitive stage undergoes development. He claimed that in the first stage, a child comparing the duration of two moving bodies attributes longer duration to the faster body, whereas in the second stage, he attributes longer duration to the slower body. It is proposed here that time-speed confusion is mediated simply by \"more is more,\" and hence, as long as the child continues to confuse time with speed, he believes \"faster\" implies longer duration. Moreover, this confusion should not differ either in form or extent from the confusion of time and many other arbitrary quantitative dimensions (e.g., intensity of light). 72 children, 4-7 years old, compared duration differing either on speed or on intensity of light. As predicted, the tasks did not differ on performance level or on error types. Moreover, the performance on both tasks was consistent with the predictions of a facet model pertaining to analysis of time problems. Confusion dissipated with age and was similar for boys and girls."}
{"id": "PMID:487887", "title": "The role of frames of reference in the development of responsiveness to shading information.", "content": "The use of frames of reference in interpreting shading information in pictures was studied with children from 3 to 8 years of age. After learning to discriminate tactually between a convexity and a concavity, the subjects were presented a photograph of the convexity and the concavity in which the only information for differential shape was provided by the relationship between the orientation of the shading on the shapes and the frames of reference. By changing the position of the subject's head, rotating the display, and changing the location of the source of illumination, the relevance of egocentric, environmental, and lighting-specified frames of reference was manipulated. Children in this age range were found to be sensitive to shading information for depth, both when that information was specified by only a single frame of reference and when all three frames of reference were relevant. Differences in the responsiveness to individual frames of reference were found: the egocentric (head-retina) frame of reference was most effective, followed by the environmental reference frame, and finally by the frame of reference based on the location of the light source in the space surrounding the photograph. Responsiveness to the environmental and lighting-based frames of reference increased with age, while responsiveness to the egocentric frame of reference was high for all age groups and did not increase with age.", "contents": "The role of frames of reference in the development of responsiveness to shading information. The use of frames of reference in interpreting shading information in pictures was studied with children from 3 to 8 years of age. After learning to discriminate tactually between a convexity and a concavity, the subjects were presented a photograph of the convexity and the concavity in which the only information for differential shape was provided by the relationship between the orientation of the shading on the shapes and the frames of reference. By changing the position of the subject's head, rotating the display, and changing the location of the source of illumination, the relevance of egocentric, environmental, and lighting-specified frames of reference was manipulated. Children in this age range were found to be sensitive to shading information for depth, both when that information was specified by only a single frame of reference and when all three frames of reference were relevant. Differences in the responsiveness to individual frames of reference were found: the egocentric (head-retina) frame of reference was most effective, followed by the environmental reference frame, and finally by the frame of reference based on the location of the light source in the space surrounding the photograph. Responsiveness to the environmental and lighting-based frames of reference increased with age, while responsiveness to the egocentric frame of reference was high for all age groups and did not increase with age."}
{"id": "PMID:487888", "title": "Configuration and position encoding in children.", "content": "2 experiments were carried out to clarify the process by which children encode briefly presented spatial positions. The task in both experiments was judging whether a test dot occupied the same position on a card as any 1 of a number of dots which had been presented tachistoscopically. Subjects were first, third, and fifth graders. In the first experiment, performance improved with grade level for stimulus arrays composed of more than 1 dot. The finding contrasts with an earlier report of only minimal developmental change in position encoding, but the procedures of the earlier study appear to have permitted a confounding of position and configural encoding. In the second experiment, position encoding was found to improve with increasing pattern goodness at all age levels. The finding attests to a powerful influence of pattern information on the perceptual system and further suggests that position information is encoded within configural information.", "contents": "Configuration and position encoding in children. 2 experiments were carried out to clarify the process by which children encode briefly presented spatial positions. The task in both experiments was judging whether a test dot occupied the same position on a card as any 1 of a number of dots which had been presented tachistoscopically. Subjects were first, third, and fifth graders. In the first experiment, performance improved with grade level for stimulus arrays composed of more than 1 dot. The finding contrasts with an earlier report of only minimal developmental change in position encoding, but the procedures of the earlier study appear to have permitted a confounding of position and configural encoding. In the second experiment, position encoding was found to improve with increasing pattern goodness at all age levels. The finding attests to a powerful influence of pattern information on the perceptual system and further suggests that position information is encoded within configural information."}
{"id": "PMID:487889", "title": "Neonatal respiratory instability and infant development.", "content": "This study examines the relationships between neonatal sleep respiratory instability and infant development. A group of 122 full-term healthy infants was observed during a nap within the first and fourth weeks of life. During each nap, a continuous polygraphic recording was obtained of respiratory activity and extraocular movements. The relative frequency and average duration of apneic pauses (greater than or equal to 2 sec) in each testing session for an infant were employed to calculate a measure of respiratory instability (PSA4) previously found to be related to the occurrence of prolonged sleep apnea. 28 of the infants in this study were maintained at home on apnea monitors. The Bayley Scales of Infant Development were administered to each infant at approximately 9 months of age. Comparisons of infants with high versus low PSA4 values and of monitored versus unmonitored infants were not strongly distorted by imbalances in birth weight, sex, race, birth order, method of feeding, Sudden Infant Death Syndrome (SIDS) sibship, parental education, age at developmental assessment, and developmental tester. Those with increased respiratory instability (PSA4 greater than or equal to -0.04) within the first week of life averaged significantly lower in mental and psychomotor development. Utilization of home apnea monitors was not significantly associated with developmental scores.", "contents": "Neonatal respiratory instability and infant development. This study examines the relationships between neonatal sleep respiratory instability and infant development. A group of 122 full-term healthy infants was observed during a nap within the first and fourth weeks of life. During each nap, a continuous polygraphic recording was obtained of respiratory activity and extraocular movements. The relative frequency and average duration of apneic pauses (greater than or equal to 2 sec) in each testing session for an infant were employed to calculate a measure of respiratory instability (PSA4) previously found to be related to the occurrence of prolonged sleep apnea. 28 of the infants in this study were maintained at home on apnea monitors. The Bayley Scales of Infant Development were administered to each infant at approximately 9 months of age. Comparisons of infants with high versus low PSA4 values and of monitored versus unmonitored infants were not strongly distorted by imbalances in birth weight, sex, race, birth order, method of feeding, Sudden Infant Death Syndrome (SIDS) sibship, parental education, age at developmental assessment, and developmental tester. Those with increased respiratory instability (PSA4 greater than or equal to -0.04) within the first week of life averaged significantly lower in mental and psychomotor development. Utilization of home apnea monitors was not significantly associated with developmental scores."}
{"id": "PMID:487890", "title": "The temporal relationship between infant heart rate acceleration and crying in an aversive situation.", "content": "The temporal relationship between heart rate (HR) acceleration and crying was examined in 16 8-16-month-old infants. Consistently, the HR acceleration began well before the onset of crying, suggesting that such acceleration is not merely a by-product of crying. The accelerations observed were above and beyond a return to baseline following orienting. The crying itself validates the association between these instances of HR acceleration and negative effect.", "contents": "The temporal relationship between infant heart rate acceleration and crying in an aversive situation. The temporal relationship between heart rate (HR) acceleration and crying was examined in 16 8-16-month-old infants. Consistently, the HR acceleration began well before the onset of crying, suggesting that such acceleration is not merely a by-product of crying. The accelerations observed were above and beyond a return to baseline following orienting. The crying itself validates the association between these instances of HR acceleration and negative effect."}
{"id": "PMID:487891", "title": "Serial habituation or regression to the mean?", "content": "The serial habituation hypothesis states that the order that infants fixate and habituate to component features of a complex form is determined by the salience of those features. Infants' fixation of the most salient feature of a complex form is reduced after exposure to that complex form, supporting the serial habituation hypothesis. Regression to the mean can also explain this result. In the present study, 4-month-olds were either presented with a complex form containing the relevant feature, or they were presented with another complex form lacking that feature. If the reduction in fixation to the most salient feature is due to the presentation of the complex form, then a reduction in fixation should only be observed in the condition in which the complex form containing that feature was presented. A reduction was observed in both conditions supporting a regression to the mean interpretation of the data.", "contents": "Serial habituation or regression to the mean? The serial habituation hypothesis states that the order that infants fixate and habituate to component features of a complex form is determined by the salience of those features. Infants' fixation of the most salient feature of a complex form is reduced after exposure to that complex form, supporting the serial habituation hypothesis. Regression to the mean can also explain this result. In the present study, 4-month-olds were either presented with a complex form containing the relevant feature, or they were presented with another complex form lacking that feature. If the reduction in fixation to the most salient feature is due to the presentation of the complex form, then a reduction in fixation should only be observed in the condition in which the complex form containing that feature was presented. A reduction was observed in both conditions supporting a regression to the mean interpretation of the data."}
{"id": "PMID:487892", "title": "Matching of auditory-visual and temporal-spatial information by seven- and nine-year-old children.", "content": "Techniques of presenting information temporally in the auditory and visual modalities and spatially in the visual modality were used to assess information processing in 7- and 9-year-old children. The results of the study were interpreted as contradictory to the view that modalities are specialized for the processing of distinct forms of information. Viewed developmentally with related literature, however, the study also suggests that some specialization of modalities may be found in the preschool years, with a school-age shift to nonspecialization.", "contents": "Matching of auditory-visual and temporal-spatial information by seven- and nine-year-old children. Techniques of presenting information temporally in the auditory and visual modalities and spatially in the visual modality were used to assess information processing in 7- and 9-year-old children. The results of the study were interpreted as contradictory to the view that modalities are specialized for the processing of distinct forms of information. Viewed developmentally with related literature, however, the study also suggests that some specialization of modalities may be found in the preschool years, with a school-age shift to nonspecialization."}
{"id": "PMID:487893", "title": "The development of cognitive maps of large- and small-scale spaces.", "content": "Kindergartners, second, and fifth graders made repeated trips through a large- or small-scale model town, and then constructed from memory the layout of buildings in either a large- or small-scale space. Accuracy of construction increased as a function of developmental level and repeated trips through the town. Children's constructions were most accurate when they were tested in the same-scale environment as that in which they developed their spatial knowledge; accuracy was impaired significantly only when children were exposed to a small space and then reconstructed in a large space. Results were interpreted in terms of a \"competence-load trade-off.\"", "contents": "The development of cognitive maps of large- and small-scale spaces. Kindergartners, second, and fifth graders made repeated trips through a large- or small-scale model town, and then constructed from memory the layout of buildings in either a large- or small-scale space. Accuracy of construction increased as a function of developmental level and repeated trips through the town. Children's constructions were most accurate when they were tested in the same-scale environment as that in which they developed their spatial knowledge; accuracy was impaired significantly only when children were exposed to a small space and then reconstructed in a large space. Results were interpreted in terms of a \"competence-load trade-off.\""}
{"id": "PMID:487894", "title": "Doctor or nurse: children's perception of sex typed occupations.", "content": "128 children between ages of 5 and 6 years were shown 4 films depicting all possible combinations of female and male physicians and nurses. Results showed that when confronted with counter-stereotypical occupational portrayals, children were likely to relabel them into the typical instance of the male physician and the female nurse. There was a stronger tendency for the subjects to relabel the male nurse than to relabel the female physician. The children's relabeling of the roles presented was not due to inattention to the stimulus materials (videotapes). Neither sex, nor age, nor the number of physician visits in the last year were related to the frequency of relabeling. Maternal employment and exposure to real male nurses were related to correct identification of the male nurse and the female physician. The results suggest that the relabeling and its asymmetric character may be due to the differential exposure of children to female physicians and male nurses.", "contents": "Doctor or nurse: children's perception of sex typed occupations. 128 children between ages of 5 and 6 years were shown 4 films depicting all possible combinations of female and male physicians and nurses. Results showed that when confronted with counter-stereotypical occupational portrayals, children were likely to relabel them into the typical instance of the male physician and the female nurse. There was a stronger tendency for the subjects to relabel the male nurse than to relabel the female physician. The children's relabeling of the roles presented was not due to inattention to the stimulus materials (videotapes). Neither sex, nor age, nor the number of physician visits in the last year were related to the frequency of relabeling. Maternal employment and exposure to real male nurses were related to correct identification of the male nurse and the female physician. The results suggest that the relabeling and its asymmetric character may be due to the differential exposure of children to female physicians and male nurses."}
{"id": "PMID:487905", "title": "Genetic microsurgery by laser: establishment of a clonal population of rat kangaroo cells (PTK2) with a directed deficiency in a chromosomal nucleolar organizer.", "content": "An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells.", "contents": "Genetic microsurgery by laser: establishment of a clonal population of rat kangaroo cells (PTK2) with a directed deficiency in a chromosomal nucleolar organizer. An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells."}
{"id": "PMID:487906", "title": "Difference between diploid and aneuploid Chinese hamster cells in replication at mid-S-phase.", "content": "Following partial synchronization of the heteroploid Chinese hamster cell line V-79 and of normal diploid lung fibroblasts of the Chinese hamster in culture, their DNA replication during S-phase aws compared by means of a BrdU-incorporation/thymidine pulse technique and Hoechst-Giemsa differential staining of metaphase chromosomes. This comparison indirectly shows the S-phase of the heteroploid cells of V-79 to be 2 h shorter than the diploid cell S-phase. When the thymidine pulse is applied to diploid lung fibroblasts at mid-S-phase, differential staining colours metaphase chromosomes a pale blue. Performing the corresponding experiment with V-79 cells, neither a pale blue nor dark red staining is obtained, but rather an intermediate shade, showing prominently dark staining regions in parts. The pause in DNA synthesis observed at mid-S-phase of the diploid Chinese hamster lung fibroblasts seems to be omitted at mid-S-phase of the V-79 cells.", "contents": "Difference between diploid and aneuploid Chinese hamster cells in replication at mid-S-phase. Following partial synchronization of the heteroploid Chinese hamster cell line V-79 and of normal diploid lung fibroblasts of the Chinese hamster in culture, their DNA replication during S-phase aws compared by means of a BrdU-incorporation/thymidine pulse technique and Hoechst-Giemsa differential staining of metaphase chromosomes. This comparison indirectly shows the S-phase of the heteroploid cells of V-79 to be 2 h shorter than the diploid cell S-phase. When the thymidine pulse is applied to diploid lung fibroblasts at mid-S-phase, differential staining colours metaphase chromosomes a pale blue. Performing the corresponding experiment with V-79 cells, neither a pale blue nor dark red staining is obtained, but rather an intermediate shade, showing prominently dark staining regions in parts. The pause in DNA synthesis observed at mid-S-phase of the diploid Chinese hamster lung fibroblasts seems to be omitted at mid-S-phase of the V-79 cells."}
{"id": "PMID:487907", "title": "Internuclear control of DNA synthesis in exconjugant cells of Paramecium caudatum.", "content": "An exconjugant cell of Paramecium caudatum has two kinds of macronuclei, fragmented prezygotic macronuclei and postzygotic new macronuclei (anlagen). Although the DNA synthesis in the fragmented prezygotic macronucleus continues until the third cell cycle after conjugation, selective suppression of the DNA synthesis in the prezygotic macronucleus takes place at the fourth cell cycle. The inhibition of DNA synthesis in prezygotic fragmented macronuclei is due to the presence of a postzygotic macronucleus (anlage) in the same cytoplasm because the inhibition does not occur when the postzygotic macronucleus (anlage) is removed by micromanipulation during the third or fourth cell cycle. Well-developed postzygotic macronuclei (anlagen) with full ability to divide have the ability to depress the DNA synthesis of prezygotic macronuclear fragments. The suppression of DNA synthesis in prezygotic macronuclear fragments seems to be irreversible. Competition for the limited amount of DNA precursors also plays an important role in the onset of the selective suppression of the DNA synthesis.", "contents": "Internuclear control of DNA synthesis in exconjugant cells of Paramecium caudatum. An exconjugant cell of Paramecium caudatum has two kinds of macronuclei, fragmented prezygotic macronuclei and postzygotic new macronuclei (anlagen). Although the DNA synthesis in the fragmented prezygotic macronucleus continues until the third cell cycle after conjugation, selective suppression of the DNA synthesis in the prezygotic macronucleus takes place at the fourth cell cycle. The inhibition of DNA synthesis in prezygotic fragmented macronuclei is due to the presence of a postzygotic macronucleus (anlage) in the same cytoplasm because the inhibition does not occur when the postzygotic macronucleus (anlage) is removed by micromanipulation during the third or fourth cell cycle. Well-developed postzygotic macronuclei (anlagen) with full ability to divide have the ability to depress the DNA synthesis of prezygotic macronuclear fragments. The suppression of DNA synthesis in prezygotic macronuclear fragments seems to be irreversible. Competition for the limited amount of DNA precursors also plays an important role in the onset of the selective suppression of the DNA synthesis."}
{"id": "PMID:487908", "title": "Quantitative determination of amplified rDNA and its distribution during oogenesis in Xenopus laevis.", "content": "The number of extra-chromosomal nucleoli and their rDNA content were determined during oogenesis in Xenopus laevis. The highly variable number of nucleoli (500 to 2,500) in oocytes of the same stage and from the same female or of different stages or from different females is not a measure of the extent of amplification. In all oocytes examined, a inversely proportional relation was found between the number of nucleoli in an oocyte and their mean rDNA content. These results indicate that there is no variation of the rDNA content of oocytes during oogenesis nor between oocytes of different females. The varying nucleolar numbers found in oocytes result thus from fusion and fission of pre-existing nucleoli. The determination of the rDNA content, in absolute units (35 pg), after amplification which occurs at the beginning of oogenesis, makes it possible to calculate the rDNA content of one nucleolus. This ranged from 0.7.10(-2) pg to 15.10(-2) pg, corresponding to about 500--11,000 cistrons of rDNA. No distinct size classes between these two extremes were observed.", "contents": "Quantitative determination of amplified rDNA and its distribution during oogenesis in Xenopus laevis. The number of extra-chromosomal nucleoli and their rDNA content were determined during oogenesis in Xenopus laevis. The highly variable number of nucleoli (500 to 2,500) in oocytes of the same stage and from the same female or of different stages or from different females is not a measure of the extent of amplification. In all oocytes examined, a inversely proportional relation was found between the number of nucleoli in an oocyte and their mean rDNA content. These results indicate that there is no variation of the rDNA content of oocytes during oogenesis nor between oocytes of different females. The varying nucleolar numbers found in oocytes result thus from fusion and fission of pre-existing nucleoli. The determination of the rDNA content, in absolute units (35 pg), after amplification which occurs at the beginning of oogenesis, makes it possible to calculate the rDNA content of one nucleolus. This ranged from 0.7.10(-2) pg to 15.10(-2) pg, corresponding to about 500--11,000 cistrons of rDNA. No distinct size classes between these two extremes were observed."}
{"id": "PMID:487909", "title": "Visualization of Transcription in early mouse embryos.", "content": "Transcriptional activity during early mammalian embryogenesis was examined in 2 cell, 4--8 cell and late morula/early blastocyst mouse embryos. Zona pellucida-free embryos were obtained after pronase digestion followed by a PMSF wash to inhibit proteolytic activity. The embryos were then lysed with NP40 detergent and spread for electron microscopy according to Miller and Bakken (1972). pre-mRNA transcription was observed at all stages. Comparison of growing RNP chain lengths revealed statistically significant differences in the distribution of shorter fibrils between the successive developmental stages examined. The number of shorter fibrils was lower in the 4--8 cell stage than in either of the two other stages. Transcription complexes of ribosomal type were detected only in 4--8 cell and morula/blastocyst embryos. Structures resembling replication loops were observed within chromatin from all stages. Similar loop-like structures as well as individually transcribed RNP fibrils were also occasionally found on fibres emerging from mitotic chromosomes. The results are discussed in the context of recent findings concerning genetic expression in early mouse embryos.", "contents": "Visualization of Transcription in early mouse embryos. Transcriptional activity during early mammalian embryogenesis was examined in 2 cell, 4--8 cell and late morula/early blastocyst mouse embryos. Zona pellucida-free embryos were obtained after pronase digestion followed by a PMSF wash to inhibit proteolytic activity. The embryos were then lysed with NP40 detergent and spread for electron microscopy according to Miller and Bakken (1972). pre-mRNA transcription was observed at all stages. Comparison of growing RNP chain lengths revealed statistically significant differences in the distribution of shorter fibrils between the successive developmental stages examined. The number of shorter fibrils was lower in the 4--8 cell stage than in either of the two other stages. Transcription complexes of ribosomal type were detected only in 4--8 cell and morula/blastocyst embryos. Structures resembling replication loops were observed within chromatin from all stages. Similar loop-like structures as well as individually transcribed RNP fibrils were also occasionally found on fibres emerging from mitotic chromosomes. The results are discussed in the context of recent findings concerning genetic expression in early mouse embryos."}
{"id": "PMID:487910", "title": "Expermental hybridization within the genus Triturus (Urodela: Salamandridae). III. Evidence for crossing-over, true chiasmata and chomosomal homologies in the spermatogenesis of F1 species hybrids, T. cristatus carnifex female X T. marmoratus male.", "content": "Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase contained up to 12 regular intergenomal bivalents and a corresponding number of univalents when less then 12 II. Most chiasmata were terminal or subterminal, some intercalary. Chiasmata between corresponding heterospecific chromosomes can be reported as true: real crossing over has taken place, proving the presence of primary chromosomal homologies between the 2 sets of the parental species. Evidence for recombination is based on the segregation of particular markers (i.e., subterminal C-bands and NORs) observed in certain chromosomes at metaphase II. One chromatid of single chromosomes can show the T. cristatus \"pheno-type\" and the other the T. marmoratus phenotype\". A few primary spermatocytes contain a certain number of irregular associations (intragenomal or intrahaploid bivalents, irregular intergenomal bivalents, chromosome multivalents) joined by chiasmata which can be defined as anomalous. Other abnormalities concern the occurrence of interlocked bivalents which occasionally show an anomalous exchange between heterologous chromatids. Cytogenetic criteria useful to evaluate the taxonomic relationships between different species have been discussed as well as some possible trends in chromosome evolution and speciation within the genus Triturus.", "contents": "Expermental hybridization within the genus Triturus (Urodela: Salamandridae). III. Evidence for crossing-over, true chiasmata and chomosomal homologies in the spermatogenesis of F1 species hybrids, T. cristatus carnifex female X T. marmoratus male. Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase contained up to 12 regular intergenomal bivalents and a corresponding number of univalents when less then 12 II. Most chiasmata were terminal or subterminal, some intercalary. Chiasmata between corresponding heterospecific chromosomes can be reported as true: real crossing over has taken place, proving the presence of primary chromosomal homologies between the 2 sets of the parental species. Evidence for recombination is based on the segregation of particular markers (i.e., subterminal C-bands and NORs) observed in certain chromosomes at metaphase II. One chromatid of single chromosomes can show the T. cristatus \"pheno-type\" and the other the T. marmoratus phenotype\". A few primary spermatocytes contain a certain number of irregular associations (intragenomal or intrahaploid bivalents, irregular intergenomal bivalents, chromosome multivalents) joined by chiasmata which can be defined as anomalous. Other abnormalities concern the occurrence of interlocked bivalents which occasionally show an anomalous exchange between heterologous chromatids. Cytogenetic criteria useful to evaluate the taxonomic relationships between different species have been discussed as well as some possible trends in chromosome evolution and speciation within the genus Triturus."}
{"id": "PMID:487911", "title": "Prognosis of Crohn's disease with onset in childhood or adolescence.", "content": "A long-term follow-up study of 522 patients (1955-1974) with onset of Crohn's disease under age 21 was conducted. Follow-up information was obtained for 513 (98.4%) of the patients. The mean follow-up for the entire series was 7.7 years with a greater than five-year follow-up for 67% of patients. Nearly 60% of the patients were ages 16--20 at onset of disease. Operation had been performed for 69% of the patients and 13 (2.4%) had died. Among the survivors 67% considered themselves to be in suboptimal health. Although clinical features and complications varied considerably with anatomic disease location, colonic disease generally caused more disability than small intestine location of disease. Age at onset did not correlate with clinical severity except for presence of growth retardation. Thus, this long-term follow-up study demonstrated the chronic nature of Crohn's disease with onset under age 21. It also emphasized that the disease more commonly affected adolescents than children, that operation was required in about two thirds of the patients, and that, while mortality was low, morbidity from the disease continued for many years.", "contents": "Prognosis of Crohn's disease with onset in childhood or adolescence. A long-term follow-up study of 522 patients (1955-1974) with onset of Crohn's disease under age 21 was conducted. Follow-up information was obtained for 513 (98.4%) of the patients. The mean follow-up for the entire series was 7.7 years with a greater than five-year follow-up for 67% of patients. Nearly 60% of the patients were ages 16--20 at onset of disease. Operation had been performed for 69% of the patients and 13 (2.4%) had died. Among the survivors 67% considered themselves to be in suboptimal health. Although clinical features and complications varied considerably with anatomic disease location, colonic disease generally caused more disability than small intestine location of disease. Age at onset did not correlate with clinical severity except for presence of growth retardation. Thus, this long-term follow-up study demonstrated the chronic nature of Crohn's disease with onset under age 21. It also emphasized that the disease more commonly affected adolescents than children, that operation was required in about two thirds of the patients, and that, while mortality was low, morbidity from the disease continued for many years."}
{"id": "PMID:487912", "title": "A controlled study comparing cimetidine treatment to an intensive antacid regimen in the therapy of uncomplicated duodenal ulcer.", "content": "The authors report the results of a randomized study in which comparison was made between two different kinds of treatment in patients affected by uncomplicated duodenal ulcer endoscopically diagnosed. The first group was treated with 1 g of cimetidine per day, during a period of four weeks (200 mg three times a day and 400 mg at bedtime); the second with a liquid Al-Mg antacid compound, 210 ml/day 30 ml, 1 and 3 hr after meals and 30 ml before bedtime) for four weeks. Fity-one patients were studied, 27 treated with cimetidine, 24 with antacids. At the end of the four-week period, 21 patients (77.7%) in the cimetidine group and 18 patients (75%) in the antacid group were completely healed. Benign side effects were remarked in both types of treatment, none of which made it necessary to suspend treatment. No significant variation of the basal and peak acid output before and after each kind of treatment was observed, while a slight but significant increase in fasting serum gastrin concentration was noted after treatment in the antacid group.", "contents": "A controlled study comparing cimetidine treatment to an intensive antacid regimen in the therapy of uncomplicated duodenal ulcer. The authors report the results of a randomized study in which comparison was made between two different kinds of treatment in patients affected by uncomplicated duodenal ulcer endoscopically diagnosed. The first group was treated with 1 g of cimetidine per day, during a period of four weeks (200 mg three times a day and 400 mg at bedtime); the second with a liquid Al-Mg antacid compound, 210 ml/day 30 ml, 1 and 3 hr after meals and 30 ml before bedtime) for four weeks. Fity-one patients were studied, 27 treated with cimetidine, 24 with antacids. At the end of the four-week period, 21 patients (77.7%) in the cimetidine group and 18 patients (75%) in the antacid group were completely healed. Benign side effects were remarked in both types of treatment, none of which made it necessary to suspend treatment. No significant variation of the basal and peak acid output before and after each kind of treatment was observed, while a slight but significant increase in fasting serum gastrin concentration was noted after treatment in the antacid group."}
{"id": "PMID:487913", "title": "Depressed jejunal secretion of water and ions in response to prostaglandin E1 in adult celiac disease.", "content": "In order to test in man the concept that intestinal fluid secretion originates from the crypts of Lieberk\u00fchn, we assessed the jejunal secretory effect of intraluminal prostaglandin E1 (PGE1) (0.9 microgram/kg/min) in untreated adult celiac disease (ACD, N = 7), treated ACD (N = 4) and normal subjects using the intestinal perfusion technique. In untreated ACD (1) water and solutes were malabsorbed (or secreted) in the basal period; (2) fluid and ion secretion seen during PGE1 infusion and net secretory effect of PGE1 (difference in transport between basal and PGE1 periods) were reduced (P less than 0.01); (3) the effects of PGE1 inhibition of sodium insorption, and stimulation of sodium exsorption, were decreased and abolished, respectively. In treated patients PGE1-induced secretion returned towards normal values. Our finding of a depressed secretory response to PGE1 in the face of marked crypt hypertrophy do not support the concept that crypt cells are the major site of intestinal fluid secretion.", "contents": "Depressed jejunal secretion of water and ions in response to prostaglandin E1 in adult celiac disease. In order to test in man the concept that intestinal fluid secretion originates from the crypts of Lieberk\u00fchn, we assessed the jejunal secretory effect of intraluminal prostaglandin E1 (PGE1) (0.9 microgram/kg/min) in untreated adult celiac disease (ACD, N = 7), treated ACD (N = 4) and normal subjects using the intestinal perfusion technique. In untreated ACD (1) water and solutes were malabsorbed (or secreted) in the basal period; (2) fluid and ion secretion seen during PGE1 infusion and net secretory effect of PGE1 (difference in transport between basal and PGE1 periods) were reduced (P less than 0.01); (3) the effects of PGE1 inhibition of sodium insorption, and stimulation of sodium exsorption, were decreased and abolished, respectively. In treated patients PGE1-induced secretion returned towards normal values. Our finding of a depressed secretory response to PGE1 in the face of marked crypt hypertrophy do not support the concept that crypt cells are the major site of intestinal fluid secretion."}
{"id": "PMID:487917", "title": "A chylous mesenteric cyst and a study of its contents.", "content": "A case of a patient with a large chylous cyst in the mesentery of the small intestine is presented. After excision of the cyst, the chyle was analyzed with special reference to its protein and lipid content. The composition of the chyle compared with that of intestinal lymph of experimental animals suggests that the fluid in the cyst has undergone concentration in terms of protein.", "contents": "A chylous mesenteric cyst and a study of its contents. A case of a patient with a large chylous cyst in the mesentery of the small intestine is presented. After excision of the cyst, the chyle was analyzed with special reference to its protein and lipid content. The composition of the chyle compared with that of intestinal lymph of experimental animals suggests that the fluid in the cyst has undergone concentration in terms of protein."}
{"id": "PMID:487918", "title": "An association between primary biliary cirrhosis and jejunal villous atrophy resembling celiac disease.", "content": "A 76-year-old female presented with malabsorption and was subsequently shown to have jejunal villous atrophy. There was only partial clinical and histologic recovery with gluten withdrawal, but this was thought to be due to poor adherence to the diet. The findings were considered to be consistent with adult celiac disease. During the course of investigations, she was also found to have primary biliary cirrhosis. This is the fifth recorded instance of this association and the first from North America. Because these are uncommon conditions, it is postulated that this is unlikely to be a chance association. The recognition of this coexistence is of practical importance in the investigation and treatment of patients with primary biliary cirrhosis and celiac disease.", "contents": "An association between primary biliary cirrhosis and jejunal villous atrophy resembling celiac disease. A 76-year-old female presented with malabsorption and was subsequently shown to have jejunal villous atrophy. There was only partial clinical and histologic recovery with gluten withdrawal, but this was thought to be due to poor adherence to the diet. The findings were considered to be consistent with adult celiac disease. During the course of investigations, she was also found to have primary biliary cirrhosis. This is the fifth recorded instance of this association and the first from North America. Because these are uncommon conditions, it is postulated that this is unlikely to be a chance association. The recognition of this coexistence is of practical importance in the investigation and treatment of patients with primary biliary cirrhosis and celiac disease."}
{"id": "PMID:487916", "title": "Drug effects on the liver: a tabular compilation of drugs and drug-related hepatic diseases.", "content": "Geneic and chemical names of drugs and the possible adverse effects of these drugs on the human liver were tabulated. All drugs compiled in these tables have been approved by the Federal Drug Administration. They are currently available in the United States, and they are listed in the 1979 Physicians' Desk Reference. The tables include: (1) names of drugs that have caused a characteristic morphologic change, such as cholestatic lobular hepatitis; (2) morphologic diagnoses that have been documented after administration of each drug; and (3) references that can be matched to each drug and to its effects on the liver.", "contents": "Drug effects on the liver: a tabular compilation of drugs and drug-related hepatic diseases. Geneic and chemical names of drugs and the possible adverse effects of these drugs on the human liver were tabulated. All drugs compiled in these tables have been approved by the Federal Drug Administration. They are currently available in the United States, and they are listed in the 1979 Physicians' Desk Reference. The tables include: (1) names of drugs that have caused a characteristic morphologic change, such as cholestatic lobular hepatitis; (2) morphologic diagnoses that have been documented after administration of each drug; and (3) references that can be matched to each drug and to its effects on the liver."}
{"id": "PMID:487921", "title": "Measurement of glycoprotein content and cell kinetics in preulcerous gastric mucosa.", "content": "In an attempt to evaluate the role of gastric mucosal defense factors in ulcerogenesis, we measured the levels of glycoproteins in the mucosa as well as mucosal cell turnover in the preulcerous phase and compared these parameters to the normal mucosa in the same animal. Ovalbumin-presensitized Praomys (Mastomys) natalensis were challenged in the gastric wall with ovalbumin and a gastric ulcer developed at the challenge site 3 days later as a result of a mucosal anaphylactic reaction. This model enabled us to study the events occurring at the site of a future ulceration. Gas-liquid chromatographic determination of mucosal glycoproteins showed that the normal and preulcerous mucosae had similar levels. Cell turnover, determined by [3H]thymidine incorporation, was stimulated as a result of the preulcerous anaphylactic reaction at 24 hours postchallenge whereas at 48 hours the values were not different from those obtained in controls. These results suggest that the pathogenesis of anaphylactic gastric ulcer involves a change in cell turnover but no changes in the production of gastric mucus.", "contents": "Measurement of glycoprotein content and cell kinetics in preulcerous gastric mucosa. In an attempt to evaluate the role of gastric mucosal defense factors in ulcerogenesis, we measured the levels of glycoproteins in the mucosa as well as mucosal cell turnover in the preulcerous phase and compared these parameters to the normal mucosa in the same animal. Ovalbumin-presensitized Praomys (Mastomys) natalensis were challenged in the gastric wall with ovalbumin and a gastric ulcer developed at the challenge site 3 days later as a result of a mucosal anaphylactic reaction. This model enabled us to study the events occurring at the site of a future ulceration. Gas-liquid chromatographic determination of mucosal glycoproteins showed that the normal and preulcerous mucosae had similar levels. Cell turnover, determined by [3H]thymidine incorporation, was stimulated as a result of the preulcerous anaphylactic reaction at 24 hours postchallenge whereas at 48 hours the values were not different from those obtained in controls. These results suggest that the pathogenesis of anaphylactic gastric ulcer involves a change in cell turnover but no changes in the production of gastric mucus."}
{"id": "PMID:487922", "title": "Malignancy in a 19-year experience of adult celiac disease.", "content": "Malignancy has developed in 10 of 93 patients with celiac disease who attended this hospital from 1959 to 1978. Four patients developed lymphoma, 5 a squamous carcinoma of the esophagus, and 1 an adenocarcinoma of the ileum. Because the majority of patients were ingesting varying amounts of gluten at the time of their follow-up no conclusion can be drawn about the possible role of a gluten-free diet in the prevention of malignancy.", "contents": "Malignancy in a 19-year experience of adult celiac disease. Malignancy has developed in 10 of 93 patients with celiac disease who attended this hospital from 1959 to 1978. Four patients developed lymphoma, 5 a squamous carcinoma of the esophagus, and 1 an adenocarcinoma of the ileum. Because the majority of patients were ingesting varying amounts of gluten at the time of their follow-up no conclusion can be drawn about the possible role of a gluten-free diet in the prevention of malignancy."}
{"id": "PMID:487924", "title": "Differential diagnosis of pancreatic mass lesion with percutaneous fine-needle aspiration biopsy under ultrasonic guidance.", "content": "Ultrasonically guided percutaneous fine-needle aspiration biopsy for cytological diagnosis was performed on 28 patients with ultrasonically scanned solid mass lesions of the pancreas in an attempt to make a differential diagnosis between pancreatic cancer and chronic pancreatitis. In 25 of the 28 patients, enough cells were aspirated to enable a cytological diagnosis to be made. In 19 of these 25 patients, malignant cells were obtained, while the aspirates from 6 patients contained only normal pancreatic cells. In 3 of the 28 patients, insufficient numbers of cells were obtained to allow the establishment of a cytological diagnosis. Pancreatic cancers were present in 22 of the 28 patients, and among these a positive diagnosis of cancer was made by aspiration biopsy in 86.4% (19/22). The technique resulted in no false positive diagnoses of cancer among 6 patients with chronic pancreatitis. No complications, such as bleeding, fistula formation, and elevation of the serum amylase, followed the procedure. This new method appears to be extremely useful in the diagnosis of pancreatic mass lesions.", "contents": "Differential diagnosis of pancreatic mass lesion with percutaneous fine-needle aspiration biopsy under ultrasonic guidance. Ultrasonically guided percutaneous fine-needle aspiration biopsy for cytological diagnosis was performed on 28 patients with ultrasonically scanned solid mass lesions of the pancreas in an attempt to make a differential diagnosis between pancreatic cancer and chronic pancreatitis. In 25 of the 28 patients, enough cells were aspirated to enable a cytological diagnosis to be made. In 19 of these 25 patients, malignant cells were obtained, while the aspirates from 6 patients contained only normal pancreatic cells. In 3 of the 28 patients, insufficient numbers of cells were obtained to allow the establishment of a cytological diagnosis. Pancreatic cancers were present in 22 of the 28 patients, and among these a positive diagnosis of cancer was made by aspiration biopsy in 86.4% (19/22). The technique resulted in no false positive diagnoses of cancer among 6 patients with chronic pancreatitis. No complications, such as bleeding, fistula formation, and elevation of the serum amylase, followed the procedure. This new method appears to be extremely useful in the diagnosis of pancreatic mass lesions."}
{"id": "PMID:487925", "title": "Studies on nicotinic acid interaction with bilirubin metabolism.", "content": "The mechanism by which intravenous administration of nicotinic acid (NA) increases serum unconjugated bilirubin in patients with the Gilbert's syndrome has been investigated. Studies using the technique of percutaneous transhepatic catheterization of the splenic vein and coil planet centrifuge suggested that following intravenous injection of NA some of the circulating erythrocytes were rendered osmotically fragile and trapped by the spleen and that unconjugated bilirubin increased in the splenic vein blood. In patients with liver cirrhosis, the increments of unconjugated bilirubin were closely correlated with the weights of the spleens removed for the management of varices. In rats, intravenous NA injection enhanced heme oxygenase activities in the spleen, but not uridine-5'-diphosphate (UDP)-glucuronyltransferase activity in the liver. These results are consistent with the hypothesis that NA-induced unconjugated hyperbilirubinemia is a result of complex reactions which include increased erythrocyte fragility, increased splenic heme oxygenase activity, and increased formation of bilirubin in the spleen.", "contents": "Studies on nicotinic acid interaction with bilirubin metabolism. The mechanism by which intravenous administration of nicotinic acid (NA) increases serum unconjugated bilirubin in patients with the Gilbert's syndrome has been investigated. Studies using the technique of percutaneous transhepatic catheterization of the splenic vein and coil planet centrifuge suggested that following intravenous injection of NA some of the circulating erythrocytes were rendered osmotically fragile and trapped by the spleen and that unconjugated bilirubin increased in the splenic vein blood. In patients with liver cirrhosis, the increments of unconjugated bilirubin were closely correlated with the weights of the spleens removed for the management of varices. In rats, intravenous NA injection enhanced heme oxygenase activities in the spleen, but not uridine-5'-diphosphate (UDP)-glucuronyltransferase activity in the liver. These results are consistent with the hypothesis that NA-induced unconjugated hyperbilirubinemia is a result of complex reactions which include increased erythrocyte fragility, increased splenic heme oxygenase activity, and increased formation of bilirubin in the spleen."}
{"id": "PMID:487926", "title": "Effect of hydrophobic detergent on lipid absorption in the rat and on lipid and sterol balance in the swine.", "content": "The effects of hydrophobic detergent on fat absorption in the rat were determined under two conditions. In the first, a high dose of detergent was given in a test lipid meal to rats not previously exposed to this agent. A marked delay in digestion of triglyceride in association with malabsorption was observed. In the second, a relatively small dose of detergent was given to rats pretreated with dietary supplement of detergent. No delay of digestion or uptake was observed but absorbed, reesterified lipid was noted to accumulate in the mucosa. Morphologic studies showed abnormal collections of fat droplets in the enterocytes. Sterol and fat balance studies were done on swine on chronic dietary detergent supplement. Mild steatorrhea with excess fecal excretion of neutral sterols was observed. It is concluded that hydrophobic detergents can have an inhibitory effect on both intraluminal and intracellular events of fat absorption.", "contents": "Effect of hydrophobic detergent on lipid absorption in the rat and on lipid and sterol balance in the swine. The effects of hydrophobic detergent on fat absorption in the rat were determined under two conditions. In the first, a high dose of detergent was given in a test lipid meal to rats not previously exposed to this agent. A marked delay in digestion of triglyceride in association with malabsorption was observed. In the second, a relatively small dose of detergent was given to rats pretreated with dietary supplement of detergent. No delay of digestion or uptake was observed but absorbed, reesterified lipid was noted to accumulate in the mucosa. Morphologic studies showed abnormal collections of fat droplets in the enterocytes. Sterol and fat balance studies were done on swine on chronic dietary detergent supplement. Mild steatorrhea with excess fecal excretion of neutral sterols was observed. It is concluded that hydrophobic detergents can have an inhibitory effect on both intraluminal and intracellular events of fat absorption."}
{"id": "PMID:487927", "title": "Association of cimetidine and bone-marrow suppression in man.", "content": "A 29-year old woman who was on steroids for Behcet's disease received intravenous cimetidine for severe gastrointestinal bleeding. A bone-marrow examination performed a few days prior to cimetidine treatment showed a normally active marrow. On the fifth day of cimetidine therapy, agranulocytosis (800/ml) and a very low platelet count (40,000/ml) were observed; bone-marrow failure was documented both by biopsy and aspiration. No other drug known to be myelotoxic was given. This severe hematologic side effect of cimetidine resolved within 10 days after discontinuation of the drug.", "contents": "Association of cimetidine and bone-marrow suppression in man. A 29-year old woman who was on steroids for Behcet's disease received intravenous cimetidine for severe gastrointestinal bleeding. A bone-marrow examination performed a few days prior to cimetidine treatment showed a normally active marrow. On the fifth day of cimetidine therapy, agranulocytosis (800/ml) and a very low platelet count (40,000/ml) were observed; bone-marrow failure was documented both by biopsy and aspiration. No other drug known to be myelotoxic was given. This severe hematologic side effect of cimetidine resolved within 10 days after discontinuation of the drug."}
{"id": "PMID:487941", "title": "Permanent disability from skin diseases. A study of 564 patients registered over a six year period.", "content": "An analysis of causes of permanent disability indicates which diseases most frequently result in chronicity and inability to work. A nation-wide study comprising all applications for permanent disability pension due to skin diseases in the period 1970--1976 was carried out. A total of 564 persons applied. The most common diagnosis was contact dermatitis followed by psoriasis and connective-tissue diseases. Allergic contact dermatitis alone accounted for 222 cases. 70% of these were due to nickel and chromium allergy. In all the diagnostic groups there was a preponderance of women except for hyperkeratotic hand eczema and genodermatoses. The frequency of the individual disorders in the present study is discussed in relation to their prevalence and prognosis in the literature.", "contents": "Permanent disability from skin diseases. A study of 564 patients registered over a six year period. An analysis of causes of permanent disability indicates which diseases most frequently result in chronicity and inability to work. A nation-wide study comprising all applications for permanent disability pension due to skin diseases in the period 1970--1976 was carried out. A total of 564 persons applied. The most common diagnosis was contact dermatitis followed by psoriasis and connective-tissue diseases. Allergic contact dermatitis alone accounted for 222 cases. 70% of these were due to nickel and chromium allergy. In all the diagnostic groups there was a preponderance of women except for hyperkeratotic hand eczema and genodermatoses. The frequency of the individual disorders in the present study is discussed in relation to their prevalence and prognosis in the literature."}
{"id": "PMID:487942", "title": "[Acute axillary eczema caused by mercury compounds].", "content": "Not every axillary dermatitis is caused by an allergy against corresponding cosmetics (deodorants or anti-perspirants). This is shown in a 24-year-old man with an acute contact dermatitis in the left axilla. Sensitization probably developed against mercury salts due to the regular use of thermometers which were kept in a disinfecting solution containing phenylmercuric borate. The patient was in two hospitals for a check up before heart surgery and temperature was regularly measured in the left axilla. In both clincis phenylmercuric borate was used for desinfection of the thermometers. The sensitivity was proofed by positive patch tests.", "contents": "[Acute axillary eczema caused by mercury compounds]. Not every axillary dermatitis is caused by an allergy against corresponding cosmetics (deodorants or anti-perspirants). This is shown in a 24-year-old man with an acute contact dermatitis in the left axilla. Sensitization probably developed against mercury salts due to the regular use of thermometers which were kept in a disinfecting solution containing phenylmercuric borate. The patient was in two hospitals for a check up before heart surgery and temperature was regularly measured in the left axilla. In both clincis phenylmercuric borate was used for desinfection of the thermometers. The sensitivity was proofed by positive patch tests."}
{"id": "PMID:487943", "title": "[Cold-induced skin damage].", "content": "Two cases of cold-induced skin-damage are reported. In one case cold-induced panniculitis (Adiponecrosis e frigore) in a small child was found. In the other case a Dermo-Hypodermitis in an elderly lady was found, where cold was possibly just one, even if certainly the most important factor of realisation for the disease. In both cases neither the parents, respectively the patient, thought of cold-inducement of the disease, neither were anamnestic circumstances sufficiently explorated by the prior treating doctors, so that it seems justified to demonstrate such events in relation to this subject.", "contents": "[Cold-induced skin damage]. Two cases of cold-induced skin-damage are reported. In one case cold-induced panniculitis (Adiponecrosis e frigore) in a small child was found. In the other case a Dermo-Hypodermitis in an elderly lady was found, where cold was possibly just one, even if certainly the most important factor of realisation for the disease. In both cases neither the parents, respectively the patient, thought of cold-inducement of the disease, neither were anamnestic circumstances sufficiently explorated by the prior treating doctors, so that it seems justified to demonstrate such events in relation to this subject."}
{"id": "PMID:487944", "title": "[Efficacy and tolerability of cefazedone in infectious skin diseases; determination of serum and tissue concentrations (author's transl)].", "content": "The efficacy and tolerance of Cefazedone, a new Cephalosporin derivate, were investigated in an open clinical trial on 40 patients with bacteriological skin infections. Patients with Erysipelas, impetiginized dermatoses and Pyodermia were treated during an average period of 9 days mainly with a dosage of 2X1 g i.v., in some cases with 2X2 g i.v. daily. The success of the treatment was good--very good in 92% of the cases. All pathogens isolated before treatment (Streptococci, St. aureus, E. coli) were eliminated after treatment. 2 patients showed transitory incompatibility reactions due to too rapid injections. The local and systemic tolerance were excellent in all other cases. 1 hour after i.v. injection of 1 g Cefazedone to 20 patients the mean concentration in the serum was 51 micrograms per ml and in the skin-tissue 10.6 micrograms/g.", "contents": "[Efficacy and tolerability of cefazedone in infectious skin diseases; determination of serum and tissue concentrations (author's transl)]. The efficacy and tolerance of Cefazedone, a new Cephalosporin derivate, were investigated in an open clinical trial on 40 patients with bacteriological skin infections. Patients with Erysipelas, impetiginized dermatoses and Pyodermia were treated during an average period of 9 days mainly with a dosage of 2X1 g i.v., in some cases with 2X2 g i.v. daily. The success of the treatment was good--very good in 92% of the cases. All pathogens isolated before treatment (Streptococci, St. aureus, E. coli) were eliminated after treatment. 2 patients showed transitory incompatibility reactions due to too rapid injections. The local and systemic tolerance were excellent in all other cases. 1 hour after i.v. injection of 1 g Cefazedone to 20 patients the mean concentration in the serum was 51 micrograms per ml and in the skin-tissue 10.6 micrograms/g."}
{"id": "PMID:487949", "title": "[Iodine-induced hyperthyroidism due to contrast media (author's transl)].", "content": "A prospective study in an area with endemic goitre showed 18 patients (15.1%) out of 119 investigated with iodine containing contrast media to be hyperthyroid after 28 days. Oral cholecystography led to the highest (27.9%) and infusion urography to the lowest (5.3%) rate of hyperthyroidism. Determination of the serum concentration of thyroxine, triiodothyronine, basal and stimulated thyrotropine is not a suitable method to detect the risk for hormonally euthyroid patients before a contrast media investigation.", "contents": "[Iodine-induced hyperthyroidism due to contrast media (author's transl)]. A prospective study in an area with endemic goitre showed 18 patients (15.1%) out of 119 investigated with iodine containing contrast media to be hyperthyroid after 28 days. Oral cholecystography led to the highest (27.9%) and infusion urography to the lowest (5.3%) rate of hyperthyroidism. Determination of the serum concentration of thyroxine, triiodothyronine, basal and stimulated thyrotropine is not a suitable method to detect the risk for hormonally euthyroid patients before a contrast media investigation."}
{"id": "PMID:487950", "title": "[Possibilities of assessing cerebrovascular disease by audiometry: Comparison with Doppler ultrasound in the assessment of extracranial vascular occlusion (author's transl)].", "content": "Discrimination loss in the speech-audiogram (one-syllable test) was tested on 61 patients with cerebrovascular disease due to extracranial vessel stenosis in the area of the carotid artery distribution. In 44 of the patients (extracranial vessel stenosis or occlusion) the results were compared with the carotid angiogram and Doppler ultrasonography. There was agreement between loss of discrimination, localisation by angiography, ultrasound results and the relevant clinical findings in 32 of 36 patients. In 17 patients audiometry was performed before and after surgical reconstruction of the internal carotid artery. Among 13 patients in whom the results could be assessed, discrimination improved postoperatively in 11 (at least 20% in six, at least 10% in five). The combination of ultrasound and audiometry can be used as a screening method in unclear cases to evaluate suspected cerebrovascular disease, simplify indications for carotid angiography, and used serially, follow any further development of the vascular disease.", "contents": "[Possibilities of assessing cerebrovascular disease by audiometry: Comparison with Doppler ultrasound in the assessment of extracranial vascular occlusion (author's transl)]. Discrimination loss in the speech-audiogram (one-syllable test) was tested on 61 patients with cerebrovascular disease due to extracranial vessel stenosis in the area of the carotid artery distribution. In 44 of the patients (extracranial vessel stenosis or occlusion) the results were compared with the carotid angiogram and Doppler ultrasonography. There was agreement between loss of discrimination, localisation by angiography, ultrasound results and the relevant clinical findings in 32 of 36 patients. In 17 patients audiometry was performed before and after surgical reconstruction of the internal carotid artery. Among 13 patients in whom the results could be assessed, discrimination improved postoperatively in 11 (at least 20% in six, at least 10% in five). The combination of ultrasound and audiometry can be used as a screening method in unclear cases to evaluate suspected cerebrovascular disease, simplify indications for carotid angiography, and used serially, follow any further development of the vascular disease."}
{"id": "PMID:487951", "title": "[Acute renal failure due to diffuse cholesterol crystal embolisation during streptokinase treatment (author's transl)].", "content": "In a 65-year-old man livedo reticularis occurred in the lower half of the body two days after beginning lysis with streptokinase. This was followed by skin and toe necrosis as well as acute renal failure. Autopsy showed a severe ulcerative atherosclerosis of the aorta with cholesterol crystal embolisation in numerous organs, particularly in both kidneys. The coincidence in time leads one to assume that the streptokinase treatment encouraged acute diffuse embolisation by dissolving the protective thrombi over the ulcerative atheromatous plaques.", "contents": "[Acute renal failure due to diffuse cholesterol crystal embolisation during streptokinase treatment (author's transl)]. In a 65-year-old man livedo reticularis occurred in the lower half of the body two days after beginning lysis with streptokinase. This was followed by skin and toe necrosis as well as acute renal failure. Autopsy showed a severe ulcerative atherosclerosis of the aorta with cholesterol crystal embolisation in numerous organs, particularly in both kidneys. The coincidence in time leads one to assume that the streptokinase treatment encouraged acute diffuse embolisation by dissolving the protective thrombi over the ulcerative atheromatous plaques."}
{"id": "PMID:487958", "title": "Role of antiplatelet agents in cerebrovascular disease.", "content": "It is now generally accepted by neurologists that most transient ischaemic attacks, particularly in the carotid artery territory, have a thromboembolic basis. These emboli are, for the most part, fibrin-platelet aggregates. Others which contain atheromatous debris are more likely to produce longer lasting neurological deficits. If one assumes this hypothesis then it is reasonable to employ drugs which interfere with platelet aggregation in order to prevent cerebrovascular symptoms and signs. Acetylsalicylic acid (aspirin) prevents aggregation by inhibiting the 'release reaction' initiated by thromboxane A2. This inhibition lasts for the life of the affected platelets. Recent trials in the United States and Canada have demonstrated a positive clinical benefit from the employment of aspirin in patients suffering from transient cerebral ischaemic attacks and amaurosis fugax. There was a reduction or cessation of the attacks in both males and females and a 50% reduction of stroke morbidity and mortality in males.", "contents": "Role of antiplatelet agents in cerebrovascular disease. It is now generally accepted by neurologists that most transient ischaemic attacks, particularly in the carotid artery territory, have a thromboembolic basis. These emboli are, for the most part, fibrin-platelet aggregates. Others which contain atheromatous debris are more likely to produce longer lasting neurological deficits. If one assumes this hypothesis then it is reasonable to employ drugs which interfere with platelet aggregation in order to prevent cerebrovascular symptoms and signs. Acetylsalicylic acid (aspirin) prevents aggregation by inhibiting the 'release reaction' initiated by thromboxane A2. This inhibition lasts for the life of the affected platelets. Recent trials in the United States and Canada have demonstrated a positive clinical benefit from the employment of aspirin in patients suffering from transient cerebral ischaemic attacks and amaurosis fugax. There was a reduction or cessation of the attacks in both males and females and a 50% reduction of stroke morbidity and mortality in males."}
{"id": "PMID:487991", "title": "[Value of pharmacokinetic data during the treatment of psychoses with haloperidol].", "content": "Pharmacokinetic data of psychotropic drugs should allow a better understanding of therapeutic results and side effects. The different factors influencing liberation, absorption, protein binding, distribution, metabolism and excretion are reviewed. Then, the principal pharmacokinetic data of haloperidol are exposed with a special emphasis on the lack of information about the correlations between plasma levels and clinical effects. The role of pharmacokinetic informations in the interpretation of responses to treatment is detailed, wishing that the monitoring of haloperidol plasma levels will be more developped in a next future.", "contents": "[Value of pharmacokinetic data during the treatment of psychoses with haloperidol]. Pharmacokinetic data of psychotropic drugs should allow a better understanding of therapeutic results and side effects. The different factors influencing liberation, absorption, protein binding, distribution, metabolism and excretion are reviewed. Then, the principal pharmacokinetic data of haloperidol are exposed with a special emphasis on the lack of information about the correlations between plasma levels and clinical effects. The role of pharmacokinetic informations in the interpretation of responses to treatment is detailed, wishing that the monitoring of haloperidol plasma levels will be more developped in a next future."}
{"id": "PMID:487992", "title": "[Psychoses with painful manifestations].", "content": "Apart from pain related to nociception and pain due to sensory deafferentation, some patients complain repeatedly of some visceral, abdominal or peripheric type of pain, urging surgeons to perform iterative operations in spite of the lack of anatomical background: these patients often labelled as cenestopaths, can be included in the group of severe obsessional neurosis, pain being the main or unique obsessional symptom. Alike other compulsive symptoms of obsessional neurosis, prefrontal lobotomy, topectomy and best of all, prefrontal cryocorticectomy provide an excellent control of pain with little or no side effects.", "contents": "[Psychoses with painful manifestations]. Apart from pain related to nociception and pain due to sensory deafferentation, some patients complain repeatedly of some visceral, abdominal or peripheric type of pain, urging surgeons to perform iterative operations in spite of the lack of anatomical background: these patients often labelled as cenestopaths, can be included in the group of severe obsessional neurosis, pain being the main or unique obsessional symptom. Alike other compulsive symptoms of obsessional neurosis, prefrontal lobotomy, topectomy and best of all, prefrontal cryocorticectomy provide an excellent control of pain with little or no side effects."}
{"id": "PMID:487993", "title": "[Evaluation of the effects of psychomotor reeducation in aged patients with dementia].", "content": "Psychomotor re-education has been attempted experimentally with a population of demented patients in a geriatric ward. It draws on the methods employed at the Bel Air Psychiatric Clinic, in particular the principle of transfer of training. To assess the effect, if any, of the therapy, two psychological rating scales devised specially for demented patients, were established by the team of psychologists at the Grenoble Teaching Hospital. These rating scales are based on the theoretical notions of J. de AJURIAGUERRA and colleagues, that is, the concepts of structural analysis, of involution and homogeneity or inhomogeneity. The psychological test rating scale proposed explores four aspects of mental activity : memory, operating mechanisms, visual defects, space perception and orientation. The rating scale is described in detail, with its references, instructions and scoring.", "contents": "[Evaluation of the effects of psychomotor reeducation in aged patients with dementia]. Psychomotor re-education has been attempted experimentally with a population of demented patients in a geriatric ward. It draws on the methods employed at the Bel Air Psychiatric Clinic, in particular the principle of transfer of training. To assess the effect, if any, of the therapy, two psychological rating scales devised specially for demented patients, were established by the team of psychologists at the Grenoble Teaching Hospital. These rating scales are based on the theoretical notions of J. de AJURIAGUERRA and colleagues, that is, the concepts of structural analysis, of involution and homogeneity or inhomogeneity. The psychological test rating scale proposed explores four aspects of mental activity : memory, operating mechanisms, visual defects, space perception and orientation. The rating scale is described in detail, with its references, instructions and scoring."}
{"id": "PMID:487994", "title": "Parathyroid hormone release is not associated with acute sympathetic arousal in goats.", "content": "Physiological activation of the sympathetic adrenomedullary (SAM) axis following both thermal and non thermal stress was assessed by changes in serum norepinephrine, glucose and/or protein as well as indices of peripheral blood flow. The occurrence of elevated serum parathyroid hormone (PTH) did not reliably reflect SAM activation as might be predicted from pharmacological studies that document a beta adrenergic receptor mechanism in the parathyroid gland that mediates catecholamine stimulated release of PTH into the circulation. The beta agonist isoproterenol at 1 microgram/min for 60 min did produce a transient increase in serum PTH at 20 min. Overall, the data raise doubts about the physiological significance of the adrenergic receptor in the parathyroid gland. Significant increases in serum PTH of 67% and 109% above basal respectively were seen following ruminal loading with cold and thermoneutral water. Associated with the PTH change were increased serum phosphorus and elevated or constant serum protein and serum total calcium.", "contents": "Parathyroid hormone release is not associated with acute sympathetic arousal in goats. Physiological activation of the sympathetic adrenomedullary (SAM) axis following both thermal and non thermal stress was assessed by changes in serum norepinephrine, glucose and/or protein as well as indices of peripheral blood flow. The occurrence of elevated serum parathyroid hormone (PTH) did not reliably reflect SAM activation as might be predicted from pharmacological studies that document a beta adrenergic receptor mechanism in the parathyroid gland that mediates catecholamine stimulated release of PTH into the circulation. The beta agonist isoproterenol at 1 microgram/min for 60 min did produce a transient increase in serum PTH at 20 min. Overall, the data raise doubts about the physiological significance of the adrenergic receptor in the parathyroid gland. Significant increases in serum PTH of 67% and 109% above basal respectively were seen following ruminal loading with cold and thermoneutral water. Associated with the PTH change were increased serum phosphorus and elevated or constant serum protein and serum total calcium."}
{"id": "PMID:487997", "title": "Chromatographic and biological properties of immunoreactive somatostatin in hypothalamic and extrahypothalamic brain regions of the rat.", "content": "A sheep antiserum to somatostatin was used to develop RIA and immunoaffinity chromatography methods for the study of immunoreactive somatostatin (IRS) in brain tissue. IRS extracted from rat median eminence, anterior hypothalamic-preoptic area, amygdala, and parietal cortex bound reversibly to immunoaffinity columns, providing a technique for concentration and partial purification. Immunoaffinity purified IRS from each of the four brain regions eluted as four peaks on gel filtration chromatography. Each peak possessed biological activity, as determined by inhibitory effects on the release of GH from cultured rat anterior pituitary cells. No differences were detected by the methods employed between IRS from the anterior hypothalamic-preoptic area, which is rich in IRS-containing neuronal cell bodies, and that from the median eminence, where IRS is localized predominantly in nerve terminals.", "contents": "Chromatographic and biological properties of immunoreactive somatostatin in hypothalamic and extrahypothalamic brain regions of the rat. A sheep antiserum to somatostatin was used to develop RIA and immunoaffinity chromatography methods for the study of immunoreactive somatostatin (IRS) in brain tissue. IRS extracted from rat median eminence, anterior hypothalamic-preoptic area, amygdala, and parietal cortex bound reversibly to immunoaffinity columns, providing a technique for concentration and partial purification. Immunoaffinity purified IRS from each of the four brain regions eluted as four peaks on gel filtration chromatography. Each peak possessed biological activity, as determined by inhibitory effects on the release of GH from cultured rat anterior pituitary cells. No differences were detected by the methods employed between IRS from the anterior hypothalamic-preoptic area, which is rich in IRS-containing neuronal cell bodies, and that from the median eminence, where IRS is localized predominantly in nerve terminals."}
{"id": "PMID:487998", "title": "Dopamine levels in hypophysial stalk plasma of the rat during surges of prolactin secretion induced by cervical stimulation.", "content": "Tonic hypothalamic inhibition of PRL release is partially explainable by dopamine secretion into hypophysial portal blood. However, the probable existence of other PRL-inhibiting factors as well as PRL-releasing factors opens to question the role of dopamine in the dynamic regulation of PRL secretion. We investigated this question in the present study by measuring dopamine concentrations in hypophysial stalk blood of the rat during the surgess of PRL secretion induced by cervical stimulation. Urethane anesthesia, necessary for the surgery attendant to stalk blood collection, did not suppress the surge of PRL secretion induced by cervical stimulation 16-24 h previously. Increases in plasma PRL levels during such surges were 4- to 5-fold above baseline. Dopamine concentrations in hypophysial stalk plasma were 36% lower in cervically stimulated than in control rats during the diurnal and nocturnal PRL surges. However, dopamine levels were not different during the interval between the surges, a time at which PRL levels are similar in stimulated and control rats. To determine if the observed 36% decrease in dopamine levels might account for the associated 4- to 5-fold rise in PRL levels during surges, we treated rats with alpha-methyl-p-tyrosine to block endogenous dopamine secretion and then infused dopamine at various rates to achieve plasma dopamine concentrations throughout the physiological range. These dopamine levels significantly but incompletely suppressed PRL levels, and a 36% decrease in administered dopamine was associated with only an approximate 1.5-fold increase in plasma PRL levels. Thus, it is unlikely that changes in dopamine secretion alone can account for the increased release of PRL engendered by cervical stimulation.", "contents": "Dopamine levels in hypophysial stalk plasma of the rat during surges of prolactin secretion induced by cervical stimulation. Tonic hypothalamic inhibition of PRL release is partially explainable by dopamine secretion into hypophysial portal blood. However, the probable existence of other PRL-inhibiting factors as well as PRL-releasing factors opens to question the role of dopamine in the dynamic regulation of PRL secretion. We investigated this question in the present study by measuring dopamine concentrations in hypophysial stalk blood of the rat during the surgess of PRL secretion induced by cervical stimulation. Urethane anesthesia, necessary for the surgery attendant to stalk blood collection, did not suppress the surge of PRL secretion induced by cervical stimulation 16-24 h previously. Increases in plasma PRL levels during such surges were 4- to 5-fold above baseline. Dopamine concentrations in hypophysial stalk plasma were 36% lower in cervically stimulated than in control rats during the diurnal and nocturnal PRL surges. However, dopamine levels were not different during the interval between the surges, a time at which PRL levels are similar in stimulated and control rats. To determine if the observed 36% decrease in dopamine levels might account for the associated 4- to 5-fold rise in PRL levels during surges, we treated rats with alpha-methyl-p-tyrosine to block endogenous dopamine secretion and then infused dopamine at various rates to achieve plasma dopamine concentrations throughout the physiological range. These dopamine levels significantly but incompletely suppressed PRL levels, and a 36% decrease in administered dopamine was associated with only an approximate 1.5-fold increase in plasma PRL levels. Thus, it is unlikely that changes in dopamine secretion alone can account for the increased release of PRL engendered by cervical stimulation."}
{"id": "PMID:488001", "title": "The presence of a cytoplasmic estrogen receptor in sexually mature rabbit epididymides: comparison with the estrogen receptor in immature rabbit epididymal cytosol.", "content": "Cytosol prepared from epididymides of castrated adult rabbits contains an estrogen-specific binding component that sediments in the 4--5S region on 0.01 M KCl sucrose gradients. The estrogen-specific binding component is not detectable on sucrose gradients unless the cytosol-[3H]estradiol mixture is first extracted with charcoal to remove uncomplexed and rapidly exchangeable ligand from the sample. The macromolecular bound [3H]estradiol that remains after charcoal extraction dissociates from the receptor with a dissociation half-time of greater than 24 h and is of high affinity (Kd = 6.5 +/- 0.9 X 10(-9) M). The estrogen receptor in the adult rabbit epididymis can also be demonstrated when charcoal-extracted samples are chromatographed on Sephadex G-200. Using this system, the major estrogen-specific binder has an apparent molecular weight of less than 200,000. In contrast, the epididymal estrogen receptor in sexually immature rabbits sediments as an 8S species on low salt gradients and has an apparent molecular weight of 200,000 or greater when assessed by chromatography on Sephadex G-200. The estrogen receptor in both age groups is distributed along the length of the epididymal duct. The highest concentration of the cytoplasmic receptor in both mature and immature rabbits is in the cauda epididymidis. The age-dependent changes in the amount of the receptor suggest that it may be involved in maturational changes in the epididymis. The alterations in the physicochemical properties may reflect physiological changes in the epididymis or in the endocrine status of the animal.", "contents": "The presence of a cytoplasmic estrogen receptor in sexually mature rabbit epididymides: comparison with the estrogen receptor in immature rabbit epididymal cytosol. Cytosol prepared from epididymides of castrated adult rabbits contains an estrogen-specific binding component that sediments in the 4--5S region on 0.01 M KCl sucrose gradients. The estrogen-specific binding component is not detectable on sucrose gradients unless the cytosol-[3H]estradiol mixture is first extracted with charcoal to remove uncomplexed and rapidly exchangeable ligand from the sample. The macromolecular bound [3H]estradiol that remains after charcoal extraction dissociates from the receptor with a dissociation half-time of greater than 24 h and is of high affinity (Kd = 6.5 +/- 0.9 X 10(-9) M). The estrogen receptor in the adult rabbit epididymis can also be demonstrated when charcoal-extracted samples are chromatographed on Sephadex G-200. Using this system, the major estrogen-specific binder has an apparent molecular weight of less than 200,000. In contrast, the epididymal estrogen receptor in sexually immature rabbits sediments as an 8S species on low salt gradients and has an apparent molecular weight of 200,000 or greater when assessed by chromatography on Sephadex G-200. The estrogen receptor in both age groups is distributed along the length of the epididymal duct. The highest concentration of the cytoplasmic receptor in both mature and immature rabbits is in the cauda epididymidis. The age-dependent changes in the amount of the receptor suggest that it may be involved in maturational changes in the epididymis. The alterations in the physicochemical properties may reflect physiological changes in the epididymis or in the endocrine status of the animal."}
{"id": "PMID:488002", "title": "Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro: correlation with Leydig cell mass.", "content": "Testes from guinea pigs, rabbits, dogs, rats, and hamsters perfused in vitro with maximally stimulating concentrations of ovine LH released 9.76 +/- 2.05, 12.80 +/- 3.15, 28.94 +/- 3.01, 3.18 +/- 0.41, and 0.70 +/- 0.12 microgram testosterone (T)/h, respectively. Adjusting for differences in testicular weight did not eliminate significant (P less than 0.01) species variation in testicular capacity for T secretion in response to ovine LH. Similarly, correction for Leydig cell mass, as determined by morphometric analysis, still left significant (P less than 0.01) differences in the testosterone secretion rates in response to ovine LH for guinea pigs (262.5 +/- 38.6 micrograms T/g Leydig cell), rabbits (205.5 +/- 50.7 micrograms T/g Leydig cell), dogs (116.4 +/- 14.8 micrograms T/g Leydig cell), rats (83.55 +/- 21.80 micrograms T/g Leydig cell), and hamsters (18.24 +/- 3.55 micrograms T/g Leydig cell). The data suggest that significant between-species variation of T production in response to ovine LH is not due to quantitative differences in the mass of Leydig cells.", "contents": "Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro: correlation with Leydig cell mass. Testes from guinea pigs, rabbits, dogs, rats, and hamsters perfused in vitro with maximally stimulating concentrations of ovine LH released 9.76 +/- 2.05, 12.80 +/- 3.15, 28.94 +/- 3.01, 3.18 +/- 0.41, and 0.70 +/- 0.12 microgram testosterone (T)/h, respectively. Adjusting for differences in testicular weight did not eliminate significant (P less than 0.01) species variation in testicular capacity for T secretion in response to ovine LH. Similarly, correction for Leydig cell mass, as determined by morphometric analysis, still left significant (P less than 0.01) differences in the testosterone secretion rates in response to ovine LH for guinea pigs (262.5 +/- 38.6 micrograms T/g Leydig cell), rabbits (205.5 +/- 50.7 micrograms T/g Leydig cell), dogs (116.4 +/- 14.8 micrograms T/g Leydig cell), rats (83.55 +/- 21.80 micrograms T/g Leydig cell), and hamsters (18.24 +/- 3.55 micrograms T/g Leydig cell). The data suggest that significant between-species variation of T production in response to ovine LH is not due to quantitative differences in the mass of Leydig cells."}
{"id": "PMID:488004", "title": "Effects of the calcium antagonist verapamil on in vitro synthesis of skeletal collagen and noncollagen protein.", "content": "Effects of the calcium antagonist verapamil on the synthesis of fetal rat bone collagen and noncollagen protein were investigated in tissue culture. Protein synthesis was quantitated by measuring the incorporation of [3H]proline into collagenase-digestible (CDP) and noncollagen protein (NCP) using bacterial collagenase; [3H]proline was added for the last 2 h of culture. Verapamil (10(-5)--10(-4) M) decreased the incorporation of label into CDP and NCP after 24 h of culture; CDP formation was inhibited to a greater extent than NCP. The inhibitory response was observed in the presence and absence of unlabeled medium proline and was not associated with changes in trichloroacetic acid-extractable radioactivity. Increasing medium calcium from 1.0 to 5.0 mM did not affect the response to 10(-4) M verapamil, whereas 3.0 mM calcium abolished the response to 10(-5) M verapamil. The inhibitory effect was reversed by 48 h of control treatment subsequent to 24-h treatment with the antagonist. Verapamil did not decrease the incorporation of [3H]thymidine into DNA or [3H]uridine into RNA, nor was there any effect of the antagonist on the DNA content of cultured bones. The prostaglandin synthetase inhibitor indomethacin did not affect the response to verapamil. We conclude that a critical concentration of intracellular calcium is necessary for normal synthesis of skeletal protein in tissue culture, and that collagen may be more sensitive to changes in intracellular calcium than NCP. In addition, other ions (e.g. sodium and potassium) may also be involved in the control of skeletal protein synthesis.", "contents": "Effects of the calcium antagonist verapamil on in vitro synthesis of skeletal collagen and noncollagen protein. Effects of the calcium antagonist verapamil on the synthesis of fetal rat bone collagen and noncollagen protein were investigated in tissue culture. Protein synthesis was quantitated by measuring the incorporation of [3H]proline into collagenase-digestible (CDP) and noncollagen protein (NCP) using bacterial collagenase; [3H]proline was added for the last 2 h of culture. Verapamil (10(-5)--10(-4) M) decreased the incorporation of label into CDP and NCP after 24 h of culture; CDP formation was inhibited to a greater extent than NCP. The inhibitory response was observed in the presence and absence of unlabeled medium proline and was not associated with changes in trichloroacetic acid-extractable radioactivity. Increasing medium calcium from 1.0 to 5.0 mM did not affect the response to 10(-4) M verapamil, whereas 3.0 mM calcium abolished the response to 10(-5) M verapamil. The inhibitory effect was reversed by 48 h of control treatment subsequent to 24-h treatment with the antagonist. Verapamil did not decrease the incorporation of [3H]thymidine into DNA or [3H]uridine into RNA, nor was there any effect of the antagonist on the DNA content of cultured bones. The prostaglandin synthetase inhibitor indomethacin did not affect the response to verapamil. We conclude that a critical concentration of intracellular calcium is necessary for normal synthesis of skeletal protein in tissue culture, and that collagen may be more sensitive to changes in intracellular calcium than NCP. In addition, other ions (e.g. sodium and potassium) may also be involved in the control of skeletal protein synthesis."}
{"id": "PMID:488006", "title": "Milk ejection reflex linked to slow wave sleep in nursing rats.", "content": "Correlations between cerebral activity of nursing rats and the milk ejection reflex were studied in Sprague-Dawley rats with 15- to 17-day-old litters. The stretch reaction of the pups, which expresses the onset of milk ejection, was closely correlated with the slow sleep epochs of the mother. Once the litter started suckling, milk ejection only took place when the mother fell asleep and electroencephalographic features of slow wave sleep appeared. Milk ejection was never found during paradoxical sleep nor when the mother was awake. Sleep deprivation for 30 min impaired milk ejection in spite of continuous suckling of the nipples by the pups. If the mother was allowed to sleep immediately afterwards, ejection of milk occurred. A 24-h sleep-wakefulness pattern did not show differences between nursing and controls. Our results show that suckling, although necessary, is not enough to set off milk ejection. This reflex only appears when the mother falls asleep, suggesting that oxytocin release is linked to suckling and slow wave sleep.", "contents": "Milk ejection reflex linked to slow wave sleep in nursing rats. Correlations between cerebral activity of nursing rats and the milk ejection reflex were studied in Sprague-Dawley rats with 15- to 17-day-old litters. The stretch reaction of the pups, which expresses the onset of milk ejection, was closely correlated with the slow sleep epochs of the mother. Once the litter started suckling, milk ejection only took place when the mother fell asleep and electroencephalographic features of slow wave sleep appeared. Milk ejection was never found during paradoxical sleep nor when the mother was awake. Sleep deprivation for 30 min impaired milk ejection in spite of continuous suckling of the nipples by the pups. If the mother was allowed to sleep immediately afterwards, ejection of milk occurred. A 24-h sleep-wakefulness pattern did not show differences between nursing and controls. Our results show that suckling, although necessary, is not enough to set off milk ejection. This reflex only appears when the mother falls asleep, suggesting that oxytocin release is linked to suckling and slow wave sleep."}
{"id": "PMID:488008", "title": "Glucose infusion potentiates the acute insulin response to nonglucose stimuli during the infusion of somatostatin.", "content": "These studies assessed the ability of glucose infusions to potentiate the acute insulin response (AIR) to iv isoproterenol (12 micrograms), arginine (750 mg), or glucose (5 g) that was previously inhibited by an infusion of somatostatin (SRIF). SRIF (1.7 micrograms/min) markedly inhibited the AIR to isoproterenol (AIR before SRIF, 28 +/- 1 microU/ml; AIR during SRIF, 8 +/- microU/ml; P less than 0.025), arginine (AIR before SRIF, 6 +/- 2 microU/ml; AIR during SRIF, 1 +/- 1 microU/ml; P less than 0.01), and glucose (AIR before SRIF, 19 +/- 7 microU/ml; AIR during SRIF, 1 +/- microU/ml; P less than 0.05). The administration of a glucose infusion of 105 mg/min partially restored the AIR to isoproterenol and arginine. Glucose infused at 440 mg/min fully restored the AIR to both isoproterenol (AIR during SRIF plus glucose, 31 +/- 4 microU/ml) and arginine (AIR during SRIF plus glucose, 9 +/- 2 microU/ml). In contrast, the AIR to glucose was not affected by infusion of glucose (AIR during SRIF plus glucose, 0 +/- 1 microU/ml). In the absence of SRIF, glucose infusion potentiates the AIR to isoproterenol and arginine but not to glucose. Therefore, during SRIF infusion, glucose retains the ability to potentiate the AIR to nonglucose stimuli despite the loss of the ability to stimulate insulin release directly. These data suggest that the potentiating effects of glucose and the inhibiting effects of SRIF may be mediated by a common mechanism affecting insulin release.", "contents": "Glucose infusion potentiates the acute insulin response to nonglucose stimuli during the infusion of somatostatin. These studies assessed the ability of glucose infusions to potentiate the acute insulin response (AIR) to iv isoproterenol (12 micrograms), arginine (750 mg), or glucose (5 g) that was previously inhibited by an infusion of somatostatin (SRIF). SRIF (1.7 micrograms/min) markedly inhibited the AIR to isoproterenol (AIR before SRIF, 28 +/- 1 microU/ml; AIR during SRIF, 8 +/- microU/ml; P less than 0.025), arginine (AIR before SRIF, 6 +/- 2 microU/ml; AIR during SRIF, 1 +/- 1 microU/ml; P less than 0.01), and glucose (AIR before SRIF, 19 +/- 7 microU/ml; AIR during SRIF, 1 +/- microU/ml; P less than 0.05). The administration of a glucose infusion of 105 mg/min partially restored the AIR to isoproterenol and arginine. Glucose infused at 440 mg/min fully restored the AIR to both isoproterenol (AIR during SRIF plus glucose, 31 +/- 4 microU/ml) and arginine (AIR during SRIF plus glucose, 9 +/- 2 microU/ml). In contrast, the AIR to glucose was not affected by infusion of glucose (AIR during SRIF plus glucose, 0 +/- 1 microU/ml). In the absence of SRIF, glucose infusion potentiates the AIR to isoproterenol and arginine but not to glucose. Therefore, during SRIF infusion, glucose retains the ability to potentiate the AIR to nonglucose stimuli despite the loss of the ability to stimulate insulin release directly. These data suggest that the potentiating effects of glucose and the inhibiting effects of SRIF may be mediated by a common mechanism affecting insulin release."}
{"id": "PMID:488009", "title": "Radiochromium distribution in hypophysectomized and adrenalectomized rats.", "content": "The effects of hypophyseal and adrenal ablation (HYPOX, ADX) on trivalent radiochromium (51Cr) distribution and of GH and T4 replacement in HYPOX rats were determined. Hypophysectomy increased body retention of 51Cr by 20--35%, and hormonal replacement restored body retention to normal. Serum 51Cr was increased 300--800%, resulting in depressed tissue to serum 51Cr ratios. GH replacement partially restored the 51Cr distribution abnormalities in HYPOX rats to normal. The combination of T4 with GH enhanced the restoration of normal 51Cr distribution, and tissue to serum 51Cr ratios returned to baseline levels. ADX had no effect on body retention of 51Cr but had a small effect on increases in serum and tissue 51Cr levels. As a result, tissue to serum 51Cr ratios did not change. These data indicate that in the HYPOX state there is a marked disturbance in Cr metabolism which is due to deficiencies of both GH and T4. Whether or not adrenal hormones are envolved in 51Cr distribution will require further study.", "contents": "Radiochromium distribution in hypophysectomized and adrenalectomized rats. The effects of hypophyseal and adrenal ablation (HYPOX, ADX) on trivalent radiochromium (51Cr) distribution and of GH and T4 replacement in HYPOX rats were determined. Hypophysectomy increased body retention of 51Cr by 20--35%, and hormonal replacement restored body retention to normal. Serum 51Cr was increased 300--800%, resulting in depressed tissue to serum 51Cr ratios. GH replacement partially restored the 51Cr distribution abnormalities in HYPOX rats to normal. The combination of T4 with GH enhanced the restoration of normal 51Cr distribution, and tissue to serum 51Cr ratios returned to baseline levels. ADX had no effect on body retention of 51Cr but had a small effect on increases in serum and tissue 51Cr levels. As a result, tissue to serum 51Cr ratios did not change. These data indicate that in the HYPOX state there is a marked disturbance in Cr metabolism which is due to deficiencies of both GH and T4. Whether or not adrenal hormones are envolved in 51Cr distribution will require further study."}
{"id": "PMID:488011", "title": "Comparison between the action of estradiol and that of the antiestrogen U 11-100 A on the induction in the rat uterus of a specific protein (the induced protein).", "content": "When measured by an in vitro approach, involving incubation in the presence of labeled leucine, the rate of synthesis of the specific estrogen-induced protein (IP) after in vivo stimulation of the rat uterus by the antiestrogens U 11-100 A (UA; 1-(2-[p-(3,4-dihydro-6-methoxy-2-phenyl-1-naphtyl)-phenoxy]ethyl)pyrrolidine hydrochloride) or CI-628 (alpha-[4-pyrrolidinoethyoxy]phenyl-4-methoxy-d-nitrostilbene) was very low compared to the response measured under the same conditions after in vivo stimulation with 17 beta-estradiol (E2). In the course of investigations aimed at clarifying the role of IP in estrogen action, we have conducted similar experiments, but the labeling step aimed at detecting and measuring IP  synthesis was carried out in vivo. We have observed that UA promoted a full IP response which is lost or missed in incubated uteri. Similar results were obtained with CI-628 and tamoxifen. A comparison between IP responses obtained and measured in vivo after E2 and those after UA action revealed that the responses paralleled the number of receptors that are translocated to the nucleus by each compound. Thus, while transient after E2 treatment, the IP (or IP-like) response was maintained for as long as 12 h (as is the nuclear receptor occupancy) when UA was used as inducer. Several explanations for the disappearance of the UA-induced IP response under in vitro conditions are considered.", "contents": "Comparison between the action of estradiol and that of the antiestrogen U 11-100 A on the induction in the rat uterus of a specific protein (the induced protein). When measured by an in vitro approach, involving incubation in the presence of labeled leucine, the rate of synthesis of the specific estrogen-induced protein (IP) after in vivo stimulation of the rat uterus by the antiestrogens U 11-100 A (UA; 1-(2-[p-(3,4-dihydro-6-methoxy-2-phenyl-1-naphtyl)-phenoxy]ethyl)pyrrolidine hydrochloride) or CI-628 (alpha-[4-pyrrolidinoethyoxy]phenyl-4-methoxy-d-nitrostilbene) was very low compared to the response measured under the same conditions after in vivo stimulation with 17 beta-estradiol (E2). In the course of investigations aimed at clarifying the role of IP in estrogen action, we have conducted similar experiments, but the labeling step aimed at detecting and measuring IP  synthesis was carried out in vivo. We have observed that UA promoted a full IP response which is lost or missed in incubated uteri. Similar results were obtained with CI-628 and tamoxifen. A comparison between IP responses obtained and measured in vivo after E2 and those after UA action revealed that the responses paralleled the number of receptors that are translocated to the nucleus by each compound. Thus, while transient after E2 treatment, the IP (or IP-like) response was maintained for as long as 12 h (as is the nuclear receptor occupancy) when UA was used as inducer. Several explanations for the disappearance of the UA-induced IP response under in vitro conditions are considered."}
{"id": "PMID:488012", "title": "Endoscopic appearance of irradiated gastric mucosa.", "content": "Irradiation of the epigastric area for gastric cancer may induce actinic lesions of the stomach characterized on endoscopic examination by ulcerations, haemorrhagic gastritis, fragility of the mucosa, thickening and congestion of the gastric folds.", "contents": "Endoscopic appearance of irradiated gastric mucosa. Irradiation of the epigastric area for gastric cancer may induce actinic lesions of the stomach characterized on endoscopic examination by ulcerations, haemorrhagic gastritis, fragility of the mucosa, thickening and congestion of the gastric folds."}
{"id": "PMID:488013", "title": "Relation of intestinal metaplasia to the acid-secreting area.", "content": "The distribution of intestinal metaplasia in relation to the acid-secreting are was investigated in 66 patients not suffering from gastroduodenal diseases. Studies were made via the endoscopic Congo Red test in combination with the methylene blue staining method. In this test, the acid-secreting area is identified as a \"discolored\" area in which Congo Red turns from red to blue-black, and areas of intestinal metaplasia were observed as areas staining blue with methylen blue. A close relationship was found between the acid-secreting area and the distribution of intestinal metaplasia; in general, intestinal metaplasia tended to be more extensive and more frequent when the acid-secreting area was small. It was also found that intestinal metaplasia increased with age. However, even in patients who were over 40 years of age, intestinal metaplasia was seen in 12.5% only, if the acid-secreting area was large. Hence, it appears likely that development of intestinal metaplasia is more closely related to the acid-secreting function than to aging.", "contents": "Relation of intestinal metaplasia to the acid-secreting area. The distribution of intestinal metaplasia in relation to the acid-secreting are was investigated in 66 patients not suffering from gastroduodenal diseases. Studies were made via the endoscopic Congo Red test in combination with the methylene blue staining method. In this test, the acid-secreting area is identified as a \"discolored\" area in which Congo Red turns from red to blue-black, and areas of intestinal metaplasia were observed as areas staining blue with methylen blue. A close relationship was found between the acid-secreting area and the distribution of intestinal metaplasia; in general, intestinal metaplasia tended to be more extensive and more frequent when the acid-secreting area was small. It was also found that intestinal metaplasia increased with age. However, even in patients who were over 40 years of age, intestinal metaplasia was seen in 12.5% only, if the acid-secreting area was large. Hence, it appears likely that development of intestinal metaplasia is more closely related to the acid-secreting function than to aging."}
{"id": "PMID:488014", "title": "Diagnosis of resectable pancreatic carcinomas by means of ERCP and intraoperative fine-needle biopsy.", "content": "In 27 out of 30 resectable carcinomas of the pancreas or the periampullary region we succeeded in recognizing microscopically the malignant nature of the lesion before removing the neoplasm. This was possible 3 times preoperatively during ERCP by means of forceps biopsy taken from the tumorinfiltrated duodenal wall and with 24 patients intraoperatively by means of fine needle biopsy and cytological rapid diagnosis.", "contents": "Diagnosis of resectable pancreatic carcinomas by means of ERCP and intraoperative fine-needle biopsy. In 27 out of 30 resectable carcinomas of the pancreas or the periampullary region we succeeded in recognizing microscopically the malignant nature of the lesion before removing the neoplasm. This was possible 3 times preoperatively during ERCP by means of forceps biopsy taken from the tumorinfiltrated duodenal wall and with 24 patients intraoperatively by means of fine needle biopsy and cytological rapid diagnosis."}
{"id": "PMID:488015", "title": "Lymphangioma of the oesophagus. Diagnosis and treatment by endoscopic polypectomy.", "content": "Finding a lymphangioma in the upper digestive tract is a rather infrequent event. A case of a 64-year-old man is reported. Diagnosis was made in the course of an endoscopic oesophagogastroduodenal examination and tumor excision with a diathermy snare at the same time. Two years after resection there was no sign of recidivation of the tumor.", "contents": "Lymphangioma of the oesophagus. Diagnosis and treatment by endoscopic polypectomy. Finding a lymphangioma in the upper digestive tract is a rather infrequent event. A case of a 64-year-old man is reported. Diagnosis was made in the course of an endoscopic oesophagogastroduodenal examination and tumor excision with a diathermy snare at the same time. Two years after resection there was no sign of recidivation of the tumor."}
{"id": "PMID:488016", "title": "Obstruction of the duodenal bulb caused by gallstone perforation.", "content": "Because of acute symptoms in the upper abdomen, upper gastrointestinal endoscopy was performed in a 75-year-old female patient. A large (5 cm x 10 cm) perforated gallstone was embedded in the duodenum, causing complete obstruction of the duodenal bulb. The stone was removed via pylorotomy. A fistula remained between the gallbladder and the duodenum. Cholecystectomy was not carried out because of the unfavourable cardiac condition of the patients. There was no complications.", "contents": "Obstruction of the duodenal bulb caused by gallstone perforation. Because of acute symptoms in the upper abdomen, upper gastrointestinal endoscopy was performed in a 75-year-old female patient. A large (5 cm x 10 cm) perforated gallstone was embedded in the duodenum, causing complete obstruction of the duodenal bulb. The stone was removed via pylorotomy. A fistula remained between the gallbladder and the duodenum. Cholecystectomy was not carried out because of the unfavourable cardiac condition of the patients. There was no complications."}
{"id": "PMID:488017", "title": "The diagnosis of post-cholecystectomy cystic duct fistula utilizing endoscopic retrograde cholangiopancreatography.", "content": "A patient developed persistent right upper quadrant pain and tenderness following cholecystectomy. An endoscopic retrograde cholangiopancreatogram showed leakage of bile through the cystic duct remnant into the subhepatic space. The endoscopic retrograde cholangiopancreatographic findings (ERCP) are described.", "contents": "The diagnosis of post-cholecystectomy cystic duct fistula utilizing endoscopic retrograde cholangiopancreatography. A patient developed persistent right upper quadrant pain and tenderness following cholecystectomy. An endoscopic retrograde cholangiopancreatogram showed leakage of bile through the cystic duct remnant into the subhepatic space. The endoscopic retrograde cholangiopancreatographic findings (ERCP) are described."}
{"id": "PMID:488018", "title": "Selective endoscopic contrastography (S.E.C.): an original association of radiology and endoscopy of the small and large intestine.", "content": "A more complete diagnostic information can be obtained by \"carrying\" the contrast medium through the fibercoloscope to those sites of the bowel where neither radiology alone nor endoscopy alone succeed in solving the diagnostic problem. The indications for a selective perendoscopic contrast study during coloscopy are few, but well defined and certainly not negligible:--demonstration and assessment of stenoses (unclarified by radiology and endoscopy);--accurate evaluation of the last ileal loop;--diagnostic study of ileo-colic surgical anastomoses;--fistulous tracts. Seventy-six patients were examined, without complications. The method proved useful in 42% of cases, conclusive in 33%, useless or inconclusive in 17%, unsuccessful in 8% (technical difficulties). On this basis, selective endoscopic contrastography is considered of use whenever its specific indications apply.", "contents": "Selective endoscopic contrastography (S.E.C.): an original association of radiology and endoscopy of the small and large intestine. A more complete diagnostic information can be obtained by \"carrying\" the contrast medium through the fibercoloscope to those sites of the bowel where neither radiology alone nor endoscopy alone succeed in solving the diagnostic problem. The indications for a selective perendoscopic contrast study during coloscopy are few, but well defined and certainly not negligible:--demonstration and assessment of stenoses (unclarified by radiology and endoscopy);--accurate evaluation of the last ileal loop;--diagnostic study of ileo-colic surgical anastomoses;--fistulous tracts. Seventy-six patients were examined, without complications. The method proved useful in 42% of cases, conclusive in 33%, useless or inconclusive in 17%, unsuccessful in 8% (technical difficulties). On this basis, selective endoscopic contrastography is considered of use whenever its specific indications apply."}
{"id": "PMID:488021", "title": "Significance of endoscopy for evaluation of vesico-ureteral reflux.", "content": "The role of urethrocystoscopy in the evaluation of vesico-ureteral reflux is discussed. In addition to voiding cystourethrograms and excretory urography endoscopic examination of patients with vesico-ureteral reflux permits the determination of the extent of the uretero-vesical junction malformation. The length of the submucosal ureter, the orifice configuration and its location as well as the trigonal muscular development are parameters which lend themselves to endoscopic evaluation. Their importance in the decision of whether vesico-ureteral reflux is treated conservatively or surgically is discussed. In addition, urethrocystoscopy may give valuable information on the causes of secondary vesico-ureteral reflux.", "contents": "Significance of endoscopy for evaluation of vesico-ureteral reflux. The role of urethrocystoscopy in the evaluation of vesico-ureteral reflux is discussed. In addition to voiding cystourethrograms and excretory urography endoscopic examination of patients with vesico-ureteral reflux permits the determination of the extent of the uretero-vesical junction malformation. The length of the submucosal ureter, the orifice configuration and its location as well as the trigonal muscular development are parameters which lend themselves to endoscopic evaluation. Their importance in the decision of whether vesico-ureteral reflux is treated conservatively or surgically is discussed. In addition, urethrocystoscopy may give valuable information on the causes of secondary vesico-ureteral reflux."}
{"id": "PMID:488022", "title": "Present status of endoscopic laser techniques in urology.", "content": "Following a brief description of the physical and biomedical characteristics of laser light, a report is given on the development and present status of laser endoscopy in urology. Presently there appear to be two principal indications: i. e. certain bladder tumors and urethral strictures. Neodymium: YAG and argon ion lasers are used for both disorders. The special laser features of these instruments are described.", "contents": "Present status of endoscopic laser techniques in urology. Following a brief description of the physical and biomedical characteristics of laser light, a report is given on the development and present status of laser endoscopy in urology. Presently there appear to be two principal indications: i. e. certain bladder tumors and urethral strictures. Neodymium: YAG and argon ion lasers are used for both disorders. The special laser features of these instruments are described."}
{"id": "PMID:488023", "title": "Endoscopic procedures in the treatment of urologic disorders in children.", "content": "Cystoscopy has been a well established procedure in adult urology for years and more recently for the endoscopic evaluation of the lower urinary tract of infants and children. Utilizing small caliber cold light endoscopes and resectoscopes, therapeutic manipulations are readily possible. Among these are extraction of ureteral stones, lithotripsy of bladder calculi, transurethral resection of bladder tumors, as well as transurethral incision and resection of bladder-neck obstructions, urethral valves and urethral stenoses. The excellent results obtained in the treatment of infravesical urinary obstruction have contributed to the importance of transurethral surgery in pediatric urology.", "contents": "Endoscopic procedures in the treatment of urologic disorders in children. Cystoscopy has been a well established procedure in adult urology for years and more recently for the endoscopic evaluation of the lower urinary tract of infants and children. Utilizing small caliber cold light endoscopes and resectoscopes, therapeutic manipulations are readily possible. Among these are extraction of ureteral stones, lithotripsy of bladder calculi, transurethral resection of bladder tumors, as well as transurethral incision and resection of bladder-neck obstructions, urethral valves and urethral stenoses. The excellent results obtained in the treatment of infravesical urinary obstruction have contributed to the importance of transurethral surgery in pediatric urology."}
{"id": "PMID:488024", "title": "Fiberendoscopic evaluation of ureterosigmoidostomies.", "content": "Sixteen patients having undergone ureterosigmoidostomy have been examined using fiberoptic coloscopes. Morphology and function of both the colonic mucosa and the ureteral stumps have been evaluated by direct observation, biopsy brush cytology and i.v. injection of methylen blue. The obtained encouraging results point to this fiberendoscopic diagnostic approach as an easy and useful follow-up examination of patients with intestinal urinary diversions.", "contents": "Fiberendoscopic evaluation of ureterosigmoidostomies. Sixteen patients having undergone ureterosigmoidostomy have been examined using fiberoptic coloscopes. Morphology and function of both the colonic mucosa and the ureteral stumps have been evaluated by direct observation, biopsy brush cytology and i.v. injection of methylen blue. The obtained encouraging results point to this fiberendoscopic diagnostic approach as an easy and useful follow-up examination of patients with intestinal urinary diversions."}
{"id": "PMID:488025", "title": "Gastric involvement in Sj\u00f6gren's syndrome simulating early gastric carcinoma.", "content": "The case of a 42-year-old woman with Sj\u00f6gren's syndrome accompanied by severe gastric infiltration and interstitial nephritis is reported. Presentation was with finger stiffness, Raynaud's phenomenon and abdominal discomfort. There were endoscopic and radiological features of early cancer, type IIc (depressed type). Resected stomach showed atrophic gastritis with erosion. Histology showed focal to relatively diffuse destruction of the gastric glands with minimal intestinal metaplasia and dense collections of lymphoid cells with occasional germinal centers in the interstitium of the gastric mucosa. Renal biopsy revealed similar severe diffuse interstitial infiltration of lymphoid cells. Distal renal tubular acidosis was confirmed by sodium bicarbonate and ammonium chloride loading test. Satisfactory improvement in xerostomia, keratoconjunctivitis sicca and other laboratory data occurred on prolonged administration of adrenal steroids and cyclophosphamide.", "contents": "Gastric involvement in Sj\u00f6gren's syndrome simulating early gastric carcinoma. The case of a 42-year-old woman with Sj\u00f6gren's syndrome accompanied by severe gastric infiltration and interstitial nephritis is reported. Presentation was with finger stiffness, Raynaud's phenomenon and abdominal discomfort. There were endoscopic and radiological features of early cancer, type IIc (depressed type). Resected stomach showed atrophic gastritis with erosion. Histology showed focal to relatively diffuse destruction of the gastric glands with minimal intestinal metaplasia and dense collections of lymphoid cells with occasional germinal centers in the interstitium of the gastric mucosa. Renal biopsy revealed similar severe diffuse interstitial infiltration of lymphoid cells. Distal renal tubular acidosis was confirmed by sodium bicarbonate and ammonium chloride loading test. Satisfactory improvement in xerostomia, keratoconjunctivitis sicca and other laboratory data occurred on prolonged administration of adrenal steroids and cyclophosphamide."}
{"id": "PMID:488026", "title": "Cadmium analysis by radiochemical neutron activation analysis.", "content": "Radiochemical neutron activation analysis (RNAA) has been routinely used at the National Bureau of Standards to analyze Cd in a variety of environmentally important matrices. The method used to separate Cd from other neutron-activated products is solvent extraction. Zinc diethyldithiocarbamate [Zn(DDC)2] in chloroform will quantitatively extract Cd from an aqueous solution over a pH range from 1 to 12. In addition to the extraction of Cd, Zn(DDC)2 will also extract Cu, which can interfere with the Cd analysis by producing a high background level of radiation. This can be avoided by first extracting with Bi(DDC)3 in chloroform which removes Cu, but not Cd. Copper concentrations can, therefore, be determined in addition to Cd. This two extraction radiochemical separation procedure is very versatile and is often used as part of a larger multi-element analysis scheme. One such scheme involves the use of an inorganic-ion exchanger, Hydrated Manganese Dioxide (HMD), to retain As, Sb, Se, and Cr prior to extraction. The eluted fraction is then extracted with Bi(DDC)3 to remove Cu, and then with Zn(DDC)2 to remove Cd.", "contents": "Cadmium analysis by radiochemical neutron activation analysis. Radiochemical neutron activation analysis (RNAA) has been routinely used at the National Bureau of Standards to analyze Cd in a variety of environmentally important matrices. The method used to separate Cd from other neutron-activated products is solvent extraction. Zinc diethyldithiocarbamate [Zn(DDC)2] in chloroform will quantitatively extract Cd from an aqueous solution over a pH range from 1 to 12. In addition to the extraction of Cd, Zn(DDC)2 will also extract Cu, which can interfere with the Cd analysis by producing a high background level of radiation. This can be avoided by first extracting with Bi(DDC)3 in chloroform which removes Cu, but not Cd. Copper concentrations can, therefore, be determined in addition to Cd. This two extraction radiochemical separation procedure is very versatile and is often used as part of a larger multi-element analysis scheme. One such scheme involves the use of an inorganic-ion exchanger, Hydrated Manganese Dioxide (HMD), to retain As, Sb, Se, and Cr prior to extraction. The eluted fraction is then extracted with Bi(DDC)3 to remove Cu, and then with Zn(DDC)2 to remove Cd."}
{"id": "PMID:488027", "title": "Effects of nutritional factors on metabolism of dietary cadmium at levels similar to those of man.", "content": "Several nutrients are known to affect cadmium toxicity, but little is known about the effect of dietary nutrient levels on absorption and tissue retention of cadmium at low dietary levels, similar to those of man. Feeding gradedlevels of zinc in a casein-gelatin diet to young Japanese quail with 109Cd (as the chloride) and 0.062 ppm added cadmium decreased the cadmium concentrations in the proventriculus-ventriculus, duodenum, jejunum-ileum, and the liver, but not in the kidney. Zinc also affected some zinc, iron, manganese, and copper tissue levels. Different tissue concentration patterns of cadmium and essential minerals were obtained with two purified control diets, one based on casein-gelatin and the other on soy isolate as the principal protein sources. The data show that relatively small dietary changes can markedly affect tissue levels of cadmium and that a low intake of zinc may increase the risk to dietary cadmium exposure. The complexity of the nutrient interrelationships and their effects on cadmium require further study to define mechanisms, which may be similar to those produced by low cadmium intakes in man.", "contents": "Effects of nutritional factors on metabolism of dietary cadmium at levels similar to those of man. Several nutrients are known to affect cadmium toxicity, but little is known about the effect of dietary nutrient levels on absorption and tissue retention of cadmium at low dietary levels, similar to those of man. Feeding gradedlevels of zinc in a casein-gelatin diet to young Japanese quail with 109Cd (as the chloride) and 0.062 ppm added cadmium decreased the cadmium concentrations in the proventriculus-ventriculus, duodenum, jejunum-ileum, and the liver, but not in the kidney. Zinc also affected some zinc, iron, manganese, and copper tissue levels. Different tissue concentration patterns of cadmium and essential minerals were obtained with two purified control diets, one based on casein-gelatin and the other on soy isolate as the principal protein sources. The data show that relatively small dietary changes can markedly affect tissue levels of cadmium and that a low intake of zinc may increase the risk to dietary cadmium exposure. The complexity of the nutrient interrelationships and their effects on cadmium require further study to define mechanisms, which may be similar to those produced by low cadmium intakes in man."}
{"id": "PMID:488028", "title": "Cadmium effects in rats on tissue iron, selenium, and blood pressure; blood and hair cadmium in some oregon residents.", "content": "Exposure of rats to cadmium causes a marked depletion of iron in liver and kidney. Selenium neither counteracts or intensifies the influence of cadmium on tissue iron levels. Selenium injections protect against cadmium-induced testicular damage but cause this element to accumulate in the testes at higher concentration than in animals exposed to cadmium without selenium. Selenium injection diverts the binding of cadmium from low molecular weight proteins to high molecular weight ones. Dosing rats with selenium and cadmium or inclusion of Se or Cd in the diet did not result in altered cadmium binding in tissues, raising some questions concerning the environmental significance of these injection experiments. Addition of selenium to a diet containing cadmium decreased the accumulation of cadmium in liver and kidney, but increased its deposition in testes. The metabolism of cadmium bound to metallothionein was markedly different as compared to the inorganic salt of this element. Dietary ascorbate, but not citrate or cysteine, decreased the deposition of cadmium in rat tissues. In some low-level exposure experiments with cadmium (1 to 1000 ppb), no differences were found in the percentage of dose absorbed or rate of cadmium accumulation when provided in food versus water. Female rats tended to absorb more cadmium than males. The binding of cadmium to cytosolic proteins was found to be different between rats fed low levels of cadmium (up to 1 ppm) as compared to those fed high levels of this element (100 ppm). Cadmium was not found to contribute to hypertension in rats, and a summary of results by various investigators is presented. Blood and hair cadmium levels in Oregon residents were found to be highest in employees of a mine, and hair cadmium was found to be respectively higher in smokers than nonsmokers and in metal workers than office workers. No relationships were observed in humans between blood or hair cadmium levels and blood pressure.", "contents": "Cadmium effects in rats on tissue iron, selenium, and blood pressure; blood and hair cadmium in some oregon residents. Exposure of rats to cadmium causes a marked depletion of iron in liver and kidney. Selenium neither counteracts or intensifies the influence of cadmium on tissue iron levels. Selenium injections protect against cadmium-induced testicular damage but cause this element to accumulate in the testes at higher concentration than in animals exposed to cadmium without selenium. Selenium injection diverts the binding of cadmium from low molecular weight proteins to high molecular weight ones. Dosing rats with selenium and cadmium or inclusion of Se or Cd in the diet did not result in altered cadmium binding in tissues, raising some questions concerning the environmental significance of these injection experiments. Addition of selenium to a diet containing cadmium decreased the accumulation of cadmium in liver and kidney, but increased its deposition in testes. The metabolism of cadmium bound to metallothionein was markedly different as compared to the inorganic salt of this element. Dietary ascorbate, but not citrate or cysteine, decreased the deposition of cadmium in rat tissues. In some low-level exposure experiments with cadmium (1 to 1000 ppb), no differences were found in the percentage of dose absorbed or rate of cadmium accumulation when provided in food versus water. Female rats tended to absorb more cadmium than males. The binding of cadmium to cytosolic proteins was found to be different between rats fed low levels of cadmium (up to 1 ppm) as compared to those fed high levels of this element (100 ppm). Cadmium was not found to contribute to hypertension in rats, and a summary of results by various investigators is presented. Blood and hair cadmium levels in Oregon residents were found to be highest in employees of a mine, and hair cadmium was found to be respectively higher in smokers than nonsmokers and in metal workers than office workers. No relationships were observed in humans between blood or hair cadmium levels and blood pressure."}
{"id": "PMID:488029", "title": "Biliary excretion of cadmium.", "content": "Biliary excretion of cadmium was studied in two groups of five rats which were given repeated injections of 109Cd. The first group was given a total amount of 6 mg Cd/kg which was 6 times as much as the second group. The elimination of cadmium in bile, urine, and feces was measured 4 to 5 weeks after the last injection. The relative biliary excretion of cadmium was the same in both groupetion, which was 0.001% but, less than the total fecal elimination of cadmium, which averaged 0.034% during the same time.", "contents": "Biliary excretion of cadmium. Biliary excretion of cadmium was studied in two groups of five rats which were given repeated injections of 109Cd. The first group was given a total amount of 6 mg Cd/kg which was 6 times as much as the second group. The elimination of cadmium in bile, urine, and feces was measured 4 to 5 weeks after the last injection. The relative biliary excretion of cadmium was the same in both groupetion, which was 0.001% but, less than the total fecal elimination of cadmium, which averaged 0.034% during the same time."}
{"id": "PMID:488030", "title": "Metabolism of orally administered cadmium-metallothionein in mice.", "content": "The main source of cadmium in the diet is cereal or meat, especially in liver and kidney. Since the cadmium in both liver and kidney is bound to metallothionein, a heat-stable protein, the gastrointestinal absorption and metabolism of cadmium metallothionein (CdMt) was studied in detail. The selective renal cadmium deposition after oral CdMt was analogous to the studies on injected CdMt. Metallothionein with 109Cd or 35S-cysteine radioactive label was isolated from rat liver and administered orally (60 microgram Cd) through a gastric tube to mice (C57 BL/6J). After 4 hr, a major portion of the ingested CdMt was isolated intact from intestinal mucosal cells. However, only a small amount of cadmium bound metallothionein was present in the kidney supernatant. The protein moeity was also degraded completely in kidney. The absorption and tissue distribution of cadmium from oral-cysteine and cadmium-glutathione complexes were similar to that after oral CdCl2 in mice. These results that oral CdMt may be absorbed intact from the gastrointestinal tract and the protein is degraded during renal deposition.", "contents": "Metabolism of orally administered cadmium-metallothionein in mice. The main source of cadmium in the diet is cereal or meat, especially in liver and kidney. Since the cadmium in both liver and kidney is bound to metallothionein, a heat-stable protein, the gastrointestinal absorption and metabolism of cadmium metallothionein (CdMt) was studied in detail. The selective renal cadmium deposition after oral CdMt was analogous to the studies on injected CdMt. Metallothionein with 109Cd or 35S-cysteine radioactive label was isolated from rat liver and administered orally (60 microgram Cd) through a gastric tube to mice (C57 BL/6J). After 4 hr, a major portion of the ingested CdMt was isolated intact from intestinal mucosal cells. However, only a small amount of cadmium bound metallothionein was present in the kidney supernatant. The protein moeity was also degraded completely in kidney. The absorption and tissue distribution of cadmium from oral-cysteine and cadmium-glutathione complexes were similar to that after oral CdCl2 in mice. These results that oral CdMt may be absorbed intact from the gastrointestinal tract and the protein is degraded during renal deposition."}
{"id": "PMID:488032", "title": "Renal tubular dysfunction and abnormalities of calcium metabolism in cadmium workers.", "content": "Tubular proteinuria is generally accepted as the critical effect following long-term, low-level exposure to cadmium as seen in an industrial environment. This effect may not be of immediate importance to the health of the individual, but the significance, in terms of long-term morbidity and mortality, of the renal tubular defect of which it is an indicator is not fully understood, and certain sequelae may have remained unrecognized due to inadequate follow-up.Follow-up studies have been performed in nine of 12 workers who were initially investigated in 1962. In six of the men exposures ranged from 28 to 45 years to cadmium sulfide dust and for shorter periods in the earlier years to cadmium oxide fume and dust. These six men had tubular proteinuria when first seen, and this has persisted in the five survivors. All six men had hypercalciuria, and two of them became recurrent stone formers. One man whose urinary calcium excretion later fell to a low level more recently developed vitamin D resistant osteomalacia. In addition, each of the six men had exhibited some, but not all, of a variety of biochemical abnormalities related to other proximal renal tubular defects, and the worker who developed osteomalacia had additional evidence of a distal tubular defect. The five survivors also have evidence of slowly progressive deterioration in glomerular function.Follow-up of this small group has shown that renal tubular dysfunction in cadmium workers may continue symptom-free for long intervals, but in a proportion of cases serious clinical effects may develop after a number of years.", "contents": "Renal tubular dysfunction and abnormalities of calcium metabolism in cadmium workers. Tubular proteinuria is generally accepted as the critical effect following long-term, low-level exposure to cadmium as seen in an industrial environment. This effect may not be of immediate importance to the health of the individual, but the significance, in terms of long-term morbidity and mortality, of the renal tubular defect of which it is an indicator is not fully understood, and certain sequelae may have remained unrecognized due to inadequate follow-up.Follow-up studies have been performed in nine of 12 workers who were initially investigated in 1962. In six of the men exposures ranged from 28 to 45 years to cadmium sulfide dust and for shorter periods in the earlier years to cadmium oxide fume and dust. These six men had tubular proteinuria when first seen, and this has persisted in the five survivors. All six men had hypercalciuria, and two of them became recurrent stone formers. One man whose urinary calcium excretion later fell to a low level more recently developed vitamin D resistant osteomalacia. In addition, each of the six men had exhibited some, but not all, of a variety of biochemical abnormalities related to other proximal renal tubular defects, and the worker who developed osteomalacia had additional evidence of a distal tubular defect. The five survivors also have evidence of slowly progressive deterioration in glomerular function.Follow-up of this small group has shown that renal tubular dysfunction in cadmium workers may continue symptom-free for long intervals, but in a proportion of cases serious clinical effects may develop after a number of years."}
{"id": "PMID:488031", "title": "Metallothionein synthesis and degradation: relationship to cadmium metabolism.", "content": "Metallothionein is an integral component of the mechanism that regulates the metabolism of cadmium and zinc. The synthesis of this protein can be \"induced\" by oral or parenteral administration of either metal. The metallothionein mRNA content of liver polysomes is increased shortly after an influx of small amounts of either metal into hepatocytes. After sufficient amounts of this poly (A+) RNA have been synthesized, there is a concomitant increase in metallothionein biosynthesis and metal binding. Unlike synthesis, the degradation of metallothionein is markedly influenced by the species of metal bound. By using in vivo and in vitro techniques, it has been possible to demonstrate that resistance of metallothionein to degradation follows the order: thionein less than zinc metallothionein less than cadmium metallothionein. Moreover, while the polypeptide chains of cadmium metallothionein are degraded, it appears that liberated cadmium ions are quickly incorporated into nascent chains of thionein. The latter explains why the cadmium content of liver and kidney increases with age and environmental exposure. Since both zinc and cadmium bind to metallothionein, it appears that the binding sites provided by this inducible species provide a locus for interaction between zinc, a nutrient, and cadmium, an environmental contaminant.", "contents": "Metallothionein synthesis and degradation: relationship to cadmium metabolism. Metallothionein is an integral component of the mechanism that regulates the metabolism of cadmium and zinc. The synthesis of this protein can be \"induced\" by oral or parenteral administration of either metal. The metallothionein mRNA content of liver polysomes is increased shortly after an influx of small amounts of either metal into hepatocytes. After sufficient amounts of this poly (A+) RNA have been synthesized, there is a concomitant increase in metallothionein biosynthesis and metal binding. Unlike synthesis, the degradation of metallothionein is markedly influenced by the species of metal bound. By using in vivo and in vitro techniques, it has been possible to demonstrate that resistance of metallothionein to degradation follows the order: thionein less than zinc metallothionein less than cadmium metallothionein. Moreover, while the polypeptide chains of cadmium metallothionein are degraded, it appears that liberated cadmium ions are quickly incorporated into nascent chains of thionein. The latter explains why the cadmium content of liver and kidney increases with age and environmental exposure. Since both zinc and cadmium bind to metallothionein, it appears that the binding sites provided by this inducible species provide a locus for interaction between zinc, a nutrient, and cadmium, an environmental contaminant."}
{"id": "PMID:488033", "title": "Industrial emission of cadmium in Japan.", "content": "Direct emission of cadmium into air and water in Japan has been controlled on the basis of the Emission and Effluent Standards. Environmental quality in these media has been improved, but delayed effects of these emissions in the past are now manifest by contamination of agricultural acreage in various parts of this country. Special land improvement projects are therefore required, and cost allocation should be made on the basis of scientific assessment of natural background. An impact assessment on accidental release of potential sources of cadmium dispersed in the water system should be made on the basis of inventory surveillance. Assessment of natural background can practically be made by two means. One is to examine the vertical distribution of cadmium in a soil profile and to search for a subsurface layer in which there can be no contamination. Another is the use of the Zn/Cd ratio as an index of pollution, a value of 400 being suggested as a screening level. The transport of cadmium was found to occur principally in particulate form, and sampling and analysis of water together with suspended solid after a heavy rainfall is suggested as a useful means for assessing the character of a catchment area and for planning the improvement of land surface in mining districts.", "contents": "Industrial emission of cadmium in Japan. Direct emission of cadmium into air and water in Japan has been controlled on the basis of the Emission and Effluent Standards. Environmental quality in these media has been improved, but delayed effects of these emissions in the past are now manifest by contamination of agricultural acreage in various parts of this country. Special land improvement projects are therefore required, and cost allocation should be made on the basis of scientific assessment of natural background. An impact assessment on accidental release of potential sources of cadmium dispersed in the water system should be made on the basis of inventory surveillance. Assessment of natural background can practically be made by two means. One is to examine the vertical distribution of cadmium in a soil profile and to search for a subsurface layer in which there can be no contamination. Another is the use of the Zn/Cd ratio as an index of pollution, a value of 400 being suggested as a screening level. The transport of cadmium was found to occur principally in particulate form, and sampling and analysis of water together with suspended solid after a heavy rainfall is suggested as a useful means for assessing the character of a catchment area and for planning the improvement of land surface in mining districts."}
{"id": "PMID:488034", "title": "Mortality and cancer morbidity among cadmium-exposed workers.", "content": "Preliminary data are reported from a study of 269 cadmium-nickel battery factory workers and 94 cadmium-copper alloy factory workers. The target group comprises all workers with more than 5 years exposure to cadmium at any time since the factories started production. An internal reference group of 328 alloy factory workers without cadmium exposure was also studied. The expected number of deaths and cancers was calculated with the \"life-table\" method by using national average incidence rates for men in different age groups and at different calendar years. It was found that among the workers in the battery factory who started work before 1948 there was an increased general mortality in the 1950's mainly due to respiratory disease. The same group had an increased renal disease mortality. There was no increase in general cancer mortality or in general cancer incidence. The risk ratio for nasopharyngeal cancer incidence was 10 (two cases), which was statistically significant. For some other sites like prostate, lung and colon-rectum the risk ratios were also greater than 1 but not statistically significant. In the alloy factory there was a tendency for an increased mortality in prostatic cancer (four cases). After correction for the \"healthy worker effect\" using the reference group, the risk ratio for prostatic cancer deaths was calculated as 2.4, but this was not statistically significant. The findings in this study support the earlier reports of an association between human cadmium exposure and increased risk for prostatic cancer.", "contents": "Mortality and cancer morbidity among cadmium-exposed workers. Preliminary data are reported from a study of 269 cadmium-nickel battery factory workers and 94 cadmium-copper alloy factory workers. The target group comprises all workers with more than 5 years exposure to cadmium at any time since the factories started production. An internal reference group of 328 alloy factory workers without cadmium exposure was also studied. The expected number of deaths and cancers was calculated with the \"life-table\" method by using national average incidence rates for men in different age groups and at different calendar years. It was found that among the workers in the battery factory who started work before 1948 there was an increased general mortality in the 1950's mainly due to respiratory disease. The same group had an increased renal disease mortality. There was no increase in general cancer mortality or in general cancer incidence. The risk ratio for nasopharyngeal cancer incidence was 10 (two cases), which was statistically significant. For some other sites like prostate, lung and colon-rectum the risk ratios were also greater than 1 but not statistically significant. In the alloy factory there was a tendency for an increased mortality in prostatic cancer (four cases). After correction for the \"healthy worker effect\" using the reference group, the risk ratio for prostatic cancer deaths was calculated as 2.4, but this was not statistically significant. The findings in this study support the earlier reports of an association between human cadmium exposure and increased risk for prostatic cancer."}
{"id": "PMID:488035", "title": "Metabolic model for cadmium in man.", "content": "A metabolic model for cadmium has been formulated in terms of a flow scheme for cadmium among eight body compartments. The mathematical description of the flow of cadmium between compartments consits of a number of differential equations, and the accumulation of cadmium may thus be calculated. Coeffcients for the flow of cadmium were estimated from empirical data both from animals and man. The modelling serves two main purposes: it provides a means of using present knowledge about metabolism in order to calculate expected accumulation in critical organs and other tissues and body fluids at certain intake levels and it makes it possible to define deficiencies in our present knowledge about metabolism. Data recently collected, partly as a result of considerations related to the model, have confirmed the assumptions of a very long biological half-time in other tissues and the small excretion of cadmium via bile.", "contents": "Metabolic model for cadmium in man. A metabolic model for cadmium has been formulated in terms of a flow scheme for cadmium among eight body compartments. The mathematical description of the flow of cadmium between compartments consits of a number of differential equations, and the accumulation of cadmium may thus be calculated. Coeffcients for the flow of cadmium were estimated from empirical data both from animals and man. The modelling serves two main purposes: it provides a means of using present knowledge about metabolism in order to calculate expected accumulation in critical organs and other tissues and body fluids at certain intake levels and it makes it possible to define deficiencies in our present knowledge about metabolism. Data recently collected, partly as a result of considerations related to the model, have confirmed the assumptions of a very long biological half-time in other tissues and the small excretion of cadmium via bile."}
{"id": "PMID:488036", "title": "Pulmonary and gastrointestinal exposure to cadmium oxide dust in a battery factory.", "content": "The elimination of cadmium in feces was studied in a group of 15 male workers exposed to cadmium oxide dust in a nickel-cadmium battery factory. The elimination of cadmium in feces was on the average 619 and 268 microgram/day in seven smokers and eight nonsmokers, respectively. The corresponding ranges were 97-2577 and 31-1102 microgram/day. The cadmium concentrations in blood were significantly higher in smokers than in nonsmokers, both before and after one month of vacation. Among the smokers there was a significant decrease in the cadmium concentrations during the vacation period, but not among the nonsmokers. It was estimated that cadmium naturally occurring in food and cigarettes, cadmium excreted from the gastrointestinal tract, and cadmium transported from the lungs by mucocillary clearance to the gastrointestinal tract only could explain up to 100 microgram of the cadmium in the feces. Since even among some nonsmokers much higher values for fecal cadmium were recorded, this was interpreted as being the result of ingestion of cadmium from contaminated hands and other body surfaces. Among the smokers, direct oral contact with contaminated cigarettes or pipes is an additional factor; the smokers also inhale cadmium in the tobacco smoke from contaminated cigarettes. Part of that cadmium is transferred to the gastrointestinal tract by mucociliary clearance and also adds to the fecal cadmium.", "contents": "Pulmonary and gastrointestinal exposure to cadmium oxide dust in a battery factory. The elimination of cadmium in feces was studied in a group of 15 male workers exposed to cadmium oxide dust in a nickel-cadmium battery factory. The elimination of cadmium in feces was on the average 619 and 268 microgram/day in seven smokers and eight nonsmokers, respectively. The corresponding ranges were 97-2577 and 31-1102 microgram/day. The cadmium concentrations in blood were significantly higher in smokers than in nonsmokers, both before and after one month of vacation. Among the smokers there was a significant decrease in the cadmium concentrations during the vacation period, but not among the nonsmokers. It was estimated that cadmium naturally occurring in food and cigarettes, cadmium excreted from the gastrointestinal tract, and cadmium transported from the lungs by mucocillary clearance to the gastrointestinal tract only could explain up to 100 microgram of the cadmium in the feces. Since even among some nonsmokers much higher values for fecal cadmium were recorded, this was interpreted as being the result of ingestion of cadmium from contaminated hands and other body surfaces. Among the smokers, direct oral contact with contaminated cigarettes or pipes is an additional factor; the smokers also inhale cadmium in the tobacco smoke from contaminated cigarettes. Part of that cadmium is transferred to the gastrointestinal tract by mucociliary clearance and also adds to the fecal cadmium."}
{"id": "PMID:488037", "title": "Cadmium in forest ecosystems around lead smelters in Missouri.", "content": "The development of Missouri's new lead belt within the past decase has provided an excellent opportunity to study the dissemination and effects of heavy metals in a deciduous forest ecosystem. Primary lead smelters within the new lead belt have been identified as potential sources of cadmium as well as lead, zinc, and copper. Sintering and blast furnace operations tend to produce significant quantities of small particulates highly enriched in cadmium and other heavy metals. At one smelter, samples of stack particulate emissions indicate that as ms accompanied by 0.44 lb zinc, 4.66 lb lead, and 0.01 lb copper/hr. These point-source emissions, as well as a number of other sources of fugitive (wind blown) and waterborne emissions contribute to a significant deposition of cadmium in the surrounding forest and stream beds. Mobilization of vagrant heavy metals may be significantly increased by contact of baghouse dusts or scrubber slurries with acidic effluents emanating from acid plants designed to produce H2SO4 as a smelter by-product. Two separate drainage forks within the Crooked Creek watershed permit some comparisons of the relative contributions of cadmium by air-borne versus water-borne contaminants. Cadmium and other heavy metals have been found to accumulate in the forest litter and partially decomposed litter along stream beds. Greater solubility, lower levels of complexation with organic ligands in the litter, and greater overall mobility of cadmium compared with lead, zinc, and copper result in appreciable contributions of dissolved cadmium to the watershed runoff. The present paper attempts to define the principle sources and current levels of heavy metal contamination and summarizes the efforts undertaken by the industry to curtail the problem.", "contents": "Cadmium in forest ecosystems around lead smelters in Missouri. The development of Missouri's new lead belt within the past decase has provided an excellent opportunity to study the dissemination and effects of heavy metals in a deciduous forest ecosystem. Primary lead smelters within the new lead belt have been identified as potential sources of cadmium as well as lead, zinc, and copper. Sintering and blast furnace operations tend to produce significant quantities of small particulates highly enriched in cadmium and other heavy metals. At one smelter, samples of stack particulate emissions indicate that as ms accompanied by 0.44 lb zinc, 4.66 lb lead, and 0.01 lb copper/hr. These point-source emissions, as well as a number of other sources of fugitive (wind blown) and waterborne emissions contribute to a significant deposition of cadmium in the surrounding forest and stream beds. Mobilization of vagrant heavy metals may be significantly increased by contact of baghouse dusts or scrubber slurries with acidic effluents emanating from acid plants designed to produce H2SO4 as a smelter by-product. Two separate drainage forks within the Crooked Creek watershed permit some comparisons of the relative contributions of cadmium by air-borne versus water-borne contaminants. Cadmium and other heavy metals have been found to accumulate in the forest litter and partially decomposed litter along stream beds. Greater solubility, lower levels of complexation with organic ligands in the litter, and greater overall mobility of cadmium compared with lead, zinc, and copper result in appreciable contributions of dissolved cadmium to the watershed runoff. The present paper attempts to define the principle sources and current levels of heavy metal contamination and summarizes the efforts undertaken by the industry to curtail the problem."}
{"id": "PMID:488038", "title": "Acute effects of cadmium on the pregnant rat and embryo-fetal development.", "content": "In rats, of the Wistar-Porton strain, a single intravenous injection of 1.25 mg Cd2+ between days 9 and 15 of gestation results in a high incidence (80% of hydrocephalus, together with other malformations in the fetuses, examined on day 20. This dose is critical, since 1.1 mg Cd2+/kg is not teratogenic, while 1.35 mg Cd2+/kg kills all the embryos. Intravenous injection of Cd2+ to the pregnant rat on day 12 causes a dose-dependent inhibition of placental Zn2+ transport. At the teratogenic dose, Zn2+ transport is inhibited by about 75% at 4 hr. Thereafter, inhibition decreases with time but is still significant at 48 hr. At 20 hr after administration of Cd2+ the embryonic concentration of Zn2+ is depressed by 33%. In the whole embryo the activity of the Zn2+-dependent thymidine kinase is inhibited by about 60% at 4 hr and at 20 hr the DNA concentration is reduced significantly. Placental transport of 14C-leucine and 14C-uridine, as well as the embryonic incorporation of these precursors into protein and RNA is unaffected at least at short times after the administration of Cd2+. It is possible therefore, that the teratogenic effects of Cd2+ may be related to the inhibition of DNA synthesis in the embryo.", "contents": "Acute effects of cadmium on the pregnant rat and embryo-fetal development. In rats, of the Wistar-Porton strain, a single intravenous injection of 1.25 mg Cd2+ between days 9 and 15 of gestation results in a high incidence (80% of hydrocephalus, together with other malformations in the fetuses, examined on day 20. This dose is critical, since 1.1 mg Cd2+/kg is not teratogenic, while 1.35 mg Cd2+/kg kills all the embryos. Intravenous injection of Cd2+ to the pregnant rat on day 12 causes a dose-dependent inhibition of placental Zn2+ transport. At the teratogenic dose, Zn2+ transport is inhibited by about 75% at 4 hr. Thereafter, inhibition decreases with time but is still significant at 48 hr. At 20 hr after administration of Cd2+ the embryonic concentration of Zn2+ is depressed by 33%. In the whole embryo the activity of the Zn2+-dependent thymidine kinase is inhibited by about 60% at 4 hr and at 20 hr the DNA concentration is reduced significantly. Placental transport of 14C-leucine and 14C-uridine, as well as the embryonic incorporation of these precursors into protein and RNA is unaffected at least at short times after the administration of Cd2+. It is possible therefore, that the teratogenic effects of Cd2+ may be related to the inhibition of DNA synthesis in the embryo."}
{"id": "PMID:488039", "title": "Increase in the systolic pressure of rats chronically fed cadmium.", "content": "In our laboratory, chronically feeding cadmium to groups of rats has been reproducibly associated with average increases of 15 to 20 mm Hg in systolic pressure. A total of 497 female Long-Evans rats were continuously provided with drinking water fortified with five essential elements and containing from 0.01 to 50 ppm cadmium, as the acetate, from weaning for as long as 30 months. These rats, plus 311 matched control animals which received fortified water without added cadmium, were fed a special low-cadmium diet. All 808 rats were weighed at least monthly as a screen for cadmium toxicity, and their systolic pressures were measured every 3 or 6 months. The two lowest concentrations of cadmium tested (0.01 and 0.03 ppm) were not pressor; the three highest concentrations (10, 25, and 50 ppm) ultimately proved to be toxic. All indirect systolic pressures (each measured in triplicate) of all rats which received 0.1 to 5 ppm cadmium (i.e., nontoxic pressor doses) averaged 15.0 mm Hg more than simultaneously measured pressures of control rats. This average increase over the control pressure is extremely significant statistically, even though it seems relatively small in absolute terms. Occasionally, however, some rats had much larger than average increases in pressure; thus, 10 of 60 rats receiving from 0.1 to 0.5 ppm cadmium for 18 months had systolic pressures that were more than 50 mm Hg above the average pressure of the control rats. Cadmium-induced hypertension is not limited to females or to a particular strain. Although we have usually used one strain of female Long-Evans rat from a single source, males of the same strain and female Sprague-Dawley rats have also developed comparable hypertension. All subgroup II elements can apparently induce similar increases in systolic pressure averaging 15 to 20 mm Hg, but cadmium is pressor in much smaller amounts than mercury or zinc. Thus, to induce a demonstrable increase in pressure requires more than ten times as much divalent mercuric ion as cadmium and more than 1000 times as much zinc as cadmium. Exposure to another metal along with cadmium can markedly alter the ability of cadmium to induce hypertension. Selenium protects against the hypertension induced by twice as much cadmium. Large excesses of both zinc and copper have also inhibited the induction of hypertension by cadmium. In contrast, lead, which like cadmium, can also induce hypertension, augments rather than inhibits cadmium-induced hypertension; thus, lead and cadmium together can induce an average increase in systolic pressure in excess of 40 mm Hg, at least twice as large as is usually induced by either metal alone.", "contents": "Increase in the systolic pressure of rats chronically fed cadmium. In our laboratory, chronically feeding cadmium to groups of rats has been reproducibly associated with average increases of 15 to 20 mm Hg in systolic pressure. A total of 497 female Long-Evans rats were continuously provided with drinking water fortified with five essential elements and containing from 0.01 to 50 ppm cadmium, as the acetate, from weaning for as long as 30 months. These rats, plus 311 matched control animals which received fortified water without added cadmium, were fed a special low-cadmium diet. All 808 rats were weighed at least monthly as a screen for cadmium toxicity, and their systolic pressures were measured every 3 or 6 months. The two lowest concentrations of cadmium tested (0.01 and 0.03 ppm) were not pressor; the three highest concentrations (10, 25, and 50 ppm) ultimately proved to be toxic. All indirect systolic pressures (each measured in triplicate) of all rats which received 0.1 to 5 ppm cadmium (i.e., nontoxic pressor doses) averaged 15.0 mm Hg more than simultaneously measured pressures of control rats. This average increase over the control pressure is extremely significant statistically, even though it seems relatively small in absolute terms. Occasionally, however, some rats had much larger than average increases in pressure; thus, 10 of 60 rats receiving from 0.1 to 0.5 ppm cadmium for 18 months had systolic pressures that were more than 50 mm Hg above the average pressure of the control rats. Cadmium-induced hypertension is not limited to females or to a particular strain. Although we have usually used one strain of female Long-Evans rat from a single source, males of the same strain and female Sprague-Dawley rats have also developed comparable hypertension. All subgroup II elements can apparently induce similar increases in systolic pressure averaging 15 to 20 mm Hg, but cadmium is pressor in much smaller amounts than mercury or zinc. Thus, to induce a demonstrable increase in pressure requires more than ten times as much divalent mercuric ion as cadmium and more than 1000 times as much zinc as cadmium. Exposure to another metal along with cadmium can markedly alter the ability of cadmium to induce hypertension. Selenium protects against the hypertension induced by twice as much cadmium. Large excesses of both zinc and copper have also inhibited the induction of hypertension by cadmium. In contrast, lead, which like cadmium, can also induce hypertension, augments rather than inhibits cadmium-induced hypertension; thus, lead and cadmium together can induce an average increase in systolic pressure in excess of 40 mm Hg, at least twice as large as is usually induced by either metal alone."}
{"id": "PMID:488040", "title": "Effects of cadmium ingestion in rats with opposite genetic predisposition to hypertension.", "content": "This study was undertaken to explore the effects of chronic low-level cadmium ingestion in Dahl hypertension-resistant (R) and hypertension-sensitive (S) lines of rats. Groups of weanling female R and S rats were given 0 or 1 mg cadmium/1. in drinking water and fed either a low salt (0.4% NaCl) or a high salt (4% NaCl) diet for 28 weeks. Cadmium produced hypertension associated with gross cardiac hypertrophy and mild to moderate renal vascular changes in S, but not in R, rats on a low salt diet. Cadmium enhanced the rate and degree of development of salt-induced hypertension without exacerbating the hypercholesterolemia or renal vascular lesions normally observed in S rats on a high salt diet. Cadmium lowered circulating cholesterol levels in both lines on a low salt diet. Cadmium had no influence on growth, blood urea nitrogen concentration, plasma renin activity, tumor formation, or survivorship in R and S rats on either salt diet. This study indicates that the genetic composition is a critical determinant of the adverse effects of chronic low-level cadmium ingestion in rats. In addition to the experimental implications, these findings may have relevance to the problem of human \"essential\" hypertension.", "contents": "Effects of cadmium ingestion in rats with opposite genetic predisposition to hypertension. This study was undertaken to explore the effects of chronic low-level cadmium ingestion in Dahl hypertension-resistant (R) and hypertension-sensitive (S) lines of rats. Groups of weanling female R and S rats were given 0 or 1 mg cadmium/1. in drinking water and fed either a low salt (0.4% NaCl) or a high salt (4% NaCl) diet for 28 weeks. Cadmium produced hypertension associated with gross cardiac hypertrophy and mild to moderate renal vascular changes in S, but not in R, rats on a low salt diet. Cadmium enhanced the rate and degree of development of salt-induced hypertension without exacerbating the hypercholesterolemia or renal vascular lesions normally observed in S rats on a high salt diet. Cadmium lowered circulating cholesterol levels in both lines on a low salt diet. Cadmium had no influence on growth, blood urea nitrogen concentration, plasma renin activity, tumor formation, or survivorship in R and S rats on either salt diet. This study indicates that the genetic composition is a critical determinant of the adverse effects of chronic low-level cadmium ingestion in rats. In addition to the experimental implications, these findings may have relevance to the problem of human \"essential\" hypertension."}
{"id": "PMID:488041", "title": "Metallothionein in the extracellular fluids as an index of cadmium toxicity.", "content": "In rats injected with 5 micron mole CdCl2/kg, 5 days/week, metallothionein was detected in plasma by gel filtration chromatography as early as four weeks. The mean renal concentration of cadmium was 80 microgram/g. The excretion of cadmium in urine at this time was rather low and amounted to 0.01% of the total dose. The amount of metallothionein in plasma, as determined by 109Cd-binding to the 10,000 molecular weight fraction, increased markedly during week 14. Its excretion in urine, however, did not start until about 10 weeks, when the cadmium concentration in kidney approached a mean value of 212 microgram/g. Signs of renal toxicity were evident from glucosuria and proteinuria which became severe during the next four weeks. The excretion of cadmium in urine increased markedly and the majority of it was in the form of metallothionein. It is suggested that the appearance of metallothionein in plasma and urine can be used as specific indices of cadmium poisoning and that the assay of the protein in these fluids may be useful in screening for excessive cadmium exposure.", "contents": "Metallothionein in the extracellular fluids as an index of cadmium toxicity. In rats injected with 5 micron mole CdCl2/kg, 5 days/week, metallothionein was detected in plasma by gel filtration chromatography as early as four weeks. The mean renal concentration of cadmium was 80 microgram/g. The excretion of cadmium in urine at this time was rather low and amounted to 0.01% of the total dose. The amount of metallothionein in plasma, as determined by 109Cd-binding to the 10,000 molecular weight fraction, increased markedly during week 14. Its excretion in urine, however, did not start until about 10 weeks, when the cadmium concentration in kidney approached a mean value of 212 microgram/g. Signs of renal toxicity were evident from glucosuria and proteinuria which became severe during the next four weeks. The excretion of cadmium in urine increased markedly and the majority of it was in the form of metallothionein. It is suggested that the appearance of metallothionein in plasma and urine can be used as specific indices of cadmium poisoning and that the assay of the protein in these fluids may be useful in screening for excessive cadmium exposure."}
{"id": "PMID:488042", "title": "Studies on cadmium-induced inhibition of hepatic microsomal drug biotransformation in the rat.", "content": "Cadmium is a potent inhibitor of hepatic microsomal drug biotransformation in the rat. Male rats receiving a single intraperitoneal dose of cadmium exhibit significant decreases in hepatic microsomal metabolism of a variety of substrates. The threshold cadmium dose is 0.84 mg Cd/kg, and the effect lasts at least 28 days. Mechanistically, the inhibitory effect results from decreased cytochrome P-450 content since cadmium does not alter NADPH cytochrome c reductase activity. This effect is also observed following acute oral administration of cadmium in doses greater than 80 mg Cd/kg but is not observed following chronic administration of the metal via drinking water in concentrations of 5-200 ppm for periods ranging from 2 to 50 weeks. A tolerance to the inhibitory cadmium effect is observed if male rats are pretreated with subthreshold doses of the metal prior to the challenge cadmium dose. The degree of tolerance can be overcome by increasing the challenge dose of cadmium. Characterization of the tolerance phenomenon in terms of onset, duration, and intensity reveals a good correlation with the kinetics of metallothionein production, suggesting that the underlying basis for the tolerance phenomenon is likely the induction of metallothionein. A sex-related difference in the inhibitory effect of cadmium was observed. Cadmium did not inhibit the metabolism of hexobarbital or ethylmorphine in female rats but did inhibit that of aniline or zoxazolamine. Cadmium did not lower cytochrome P-450 content in female rats.", "contents": "Studies on cadmium-induced inhibition of hepatic microsomal drug biotransformation in the rat. Cadmium is a potent inhibitor of hepatic microsomal drug biotransformation in the rat. Male rats receiving a single intraperitoneal dose of cadmium exhibit significant decreases in hepatic microsomal metabolism of a variety of substrates. The threshold cadmium dose is 0.84 mg Cd/kg, and the effect lasts at least 28 days. Mechanistically, the inhibitory effect results from decreased cytochrome P-450 content since cadmium does not alter NADPH cytochrome c reductase activity. This effect is also observed following acute oral administration of cadmium in doses greater than 80 mg Cd/kg but is not observed following chronic administration of the metal via drinking water in concentrations of 5-200 ppm for periods ranging from 2 to 50 weeks. A tolerance to the inhibitory cadmium effect is observed if male rats are pretreated with subthreshold doses of the metal prior to the challenge cadmium dose. The degree of tolerance can be overcome by increasing the challenge dose of cadmium. Characterization of the tolerance phenomenon in terms of onset, duration, and intensity reveals a good correlation with the kinetics of metallothionein production, suggesting that the underlying basis for the tolerance phenomenon is likely the induction of metallothionein. A sex-related difference in the inhibitory effect of cadmium was observed. Cadmium did not inhibit the metabolism of hexobarbital or ethylmorphine in female rats but did inhibit that of aniline or zoxazolamine. Cadmium did not lower cytochrome P-450 content in female rats."}
{"id": "PMID:488043", "title": "Cadmium, an effector in the synthesis of thionein.", "content": "Cadmium can elicit the synthesis of thionein in liver cells independent of tissue-organ interactions. The metal diffuses across the plasma membrane and is partitioned between subcellular components in a time dependent manner such that thionein synthesis responds to levels of nonspecifically and specifically bound cytoplasmic metal. Cadmium appears to function at the transcriptional level, and the metal may act to increase the pool of specific m-RNA's.", "contents": "Cadmium, an effector in the synthesis of thionein. Cadmium can elicit the synthesis of thionein in liver cells independent of tissue-organ interactions. The metal diffuses across the plasma membrane and is partitioned between subcellular components in a time dependent manner such that thionein synthesis responds to levels of nonspecifically and specifically bound cytoplasmic metal. Cadmium appears to function at the transcriptional level, and the metal may act to increase the pool of specific m-RNA's."}
{"id": "PMID:488044", "title": "Early cellular effects of circulating cadmium-thionein on kidney proximal tubules.", "content": "Circulating cadmium-thionein (Cd-MT) is cleared from the mammalian circulatory system by filtration through the kidney glomerulus with subsequent reabsorption by kidney proximal tubules. Damage to the tubules results following uptake of Cd-MT, which is dependent upon time and the dose level of cadmium administered. Intravenous administration of 109Cd-MT at doses of 0.017 and 0.17 mg Cd/kg body weight with examination of total renal uptake of 109Cd at 0.5, 3, and 24 hr disclosed that the rate of clearance from the blood and uptake by the kidney was significantly more rapid at the 0.017 mg Cd/kg dose. Ultrastructural changes resulting from intravenous injection of either form A or B of Cd-MT were characterized by increased numbers of pinocytotic vesicles and small, dense lysosomal structures. There was no evidence of mitochondrial swelling or cell death at either 3 or 6 hr after injection. The subcellular distribution of cadmium in kidney tissue at various times after administration of Cd-MT was determined by using differential centrifugation techniques with 109Cd and in situ by using x-ray microanalysis. At 30 min after injection of Cd-MT, significant amounts of cadmium were present in lysosomal fractions indicating an interaction between the tubular lysosome system and Cd-MT prior to the onset of overt cellular toxicity. Results suggest that Cd-MT is reabsorbed and broken down by kidney tubule cells in a physiological manner with possible subsequent release of the toxic cadmium ion.", "contents": "Early cellular effects of circulating cadmium-thionein on kidney proximal tubules. Circulating cadmium-thionein (Cd-MT) is cleared from the mammalian circulatory system by filtration through the kidney glomerulus with subsequent reabsorption by kidney proximal tubules. Damage to the tubules results following uptake of Cd-MT, which is dependent upon time and the dose level of cadmium administered. Intravenous administration of 109Cd-MT at doses of 0.017 and 0.17 mg Cd/kg body weight with examination of total renal uptake of 109Cd at 0.5, 3, and 24 hr disclosed that the rate of clearance from the blood and uptake by the kidney was significantly more rapid at the 0.017 mg Cd/kg dose. Ultrastructural changes resulting from intravenous injection of either form A or B of Cd-MT were characterized by increased numbers of pinocytotic vesicles and small, dense lysosomal structures. There was no evidence of mitochondrial swelling or cell death at either 3 or 6 hr after injection. The subcellular distribution of cadmium in kidney tissue at various times after administration of Cd-MT was determined by using differential centrifugation techniques with 109Cd and in situ by using x-ray microanalysis. At 30 min after injection of Cd-MT, significant amounts of cadmium were present in lysosomal fractions indicating an interaction between the tubular lysosome system and Cd-MT prior to the onset of overt cellular toxicity. Results suggest that Cd-MT is reabsorbed and broken down by kidney tubule cells in a physiological manner with possible subsequent release of the toxic cadmium ion."}
{"id": "PMID:488047", "title": "Some aspects of cadmium flow in the U.S.", "content": "A team of Purdue University engineers and scientists has been involved in studying sources, translocation mechanisms, and fate of cadmium in the environment. One of the principal results of this work has been the development of a cadmium flow model for the U. S. which involves simulating sources, use patterns, waste treatment and recovery techniques, waste disposal options, and environmental flow mechanisms. A series of model calculations performed specify cadmium environmental flow, fate, and human exposure for a variety of use pattern, waste treatment/recovery, and disposal scenarios over a ten-year-simulation period.", "contents": "Some aspects of cadmium flow in the U.S. A team of Purdue University engineers and scientists has been involved in studying sources, translocation mechanisms, and fate of cadmium in the environment. One of the principal results of this work has been the development of a cadmium flow model for the U. S. which involves simulating sources, use patterns, waste treatment and recovery techniques, waste disposal options, and environmental flow mechanisms. A series of model calculations performed specify cadmium environmental flow, fate, and human exposure for a variety of use pattern, waste treatment/recovery, and disposal scenarios over a ten-year-simulation period."}
{"id": "PMID:488048", "title": "Cadmium from soil amended with sewage sludge: effects and residues in swine.", "content": "Liquid digested sewage sludge from a Chicago waste treatment plant was applied to experimental corn plots starting in 1968. The treatment plant received a high proportion of industrial effluent and the sludge averaged about 200 ppm Cd (dry weight). Corn grain harvested from the plots in 1974 was fed to growing swine for 56 days, and other swine were permitted to forage on the plots during the winters of 1975-76 and 1976-77. The sludge-fertilized corn contained higher concentrations of nutrient and toxic elements, but did not interfere with swine performance. Minor changes in hepatic microsomal oxidases and red blood cells accompanied significant increases in renal Cd and decreases in hepatic Fe. Swine foraging on these plots ingested considerable amounts of sludge soil and accumulated significantly higher concentrations of renal Cd. At lower rates of sludge application the swine outperformed those foraging both on control plots and those receiving heavy sludge applications in terms of weight gain, in-utero piglet survival, blood hemoglobin, and tissue Fe concentrations.", "contents": "Cadmium from soil amended with sewage sludge: effects and residues in swine. Liquid digested sewage sludge from a Chicago waste treatment plant was applied to experimental corn plots starting in 1968. The treatment plant received a high proportion of industrial effluent and the sludge averaged about 200 ppm Cd (dry weight). Corn grain harvested from the plots in 1974 was fed to growing swine for 56 days, and other swine were permitted to forage on the plots during the winters of 1975-76 and 1976-77. The sludge-fertilized corn contained higher concentrations of nutrient and toxic elements, but did not interfere with swine performance. Minor changes in hepatic microsomal oxidases and red blood cells accompanied significant increases in renal Cd and decreases in hepatic Fe. Swine foraging on these plots ingested considerable amounts of sludge soil and accumulated significantly higher concentrations of renal Cd. At lower rates of sludge application the swine outperformed those foraging both on control plots and those receiving heavy sludge applications in terms of weight gain, in-utero piglet survival, blood hemoglobin, and tissue Fe concentrations."}
{"id": "PMID:488049", "title": "Cadmium uptake from feed and its distribution to food products of livestock.", "content": "Distribution of cadmium (Cd) into the edible products of three species of food-producing animals was investigated during long-term dietary administration of supplemental cadmium chloride. Cows were exposed to 0.2 (control), 2.4, and 11.3 ppm Cd on whole ration basis for a period of three months followed by a three-month period on control ration. No accumulation of Cd occurred in milk, muscle, or bone at any of the time periods. A dose-related increase of Cd was observed in liver and kidney. The Cd concentration in these organs showed a gradual rise even when the animals were given control ration for three months after an initial three-month exposure to Cd; this observation suggests a mobilization and redistribution of this metal from other tissues. Dietary Cd levels in swine rations were 0.2, 2.4, and 10.1 ppm. The highest level of Cd produced a slightly reduced growth rate in swine. No accumulation of Cd was observed in muscle, bone, or brain. Liver and kidney showed a treatment and time-related increase in cadmium values at 6 and 12 weeks on experiment. During a three-month depletion phase after an initial three months of Cd administration, no further change in liver and kidney Cd levels was observed. White Leghorn chickens were treated by administering 0.3, 1.9, and 13.1 ppm Cd in their diets for up to 6 months. No accumulation of Cd occurred in eggs or bones. A slight increase of Cd level was observed in chicken muscle after six months of exposure. Liver and kidney had the highest levels of Cd, which showed a dose and time-related increase in these organs. No depletion of liver and kidney Cd was seen during seven weeks following a six-week treatment period. In all three species, the kidney Cd levels were severalfold higher than those of liver at all dietary levels. In swine, the renal cortex and medulla had similar Cd concentration in control animals but in all animals exposed to supplemental Cd, a dramatic rise in Cd levels in renal cortex was observed. Medullary Cd did not show a proportional time and dose-related increase in Cd levels, although the levels showed some increase. In kidney and liver of all three species the Cd levels showed a positive correlation with the amount of Cd-binding protein in these tissues. Induced levels of this metal-binding protein may explain accumulation and persistence of Cd-residues in these organs. In all three species, the concentrations of renal Cd-binding protein increased at a rate greater than those in liver. Although the food-producing animals may act as an effective filter of Cd in the case of an environmental increase of this metal, consumption of visceral organs from such animals may pose a hazard. This is particularly critical since the daily intake of Cd in human diet is already estimated to exceed the tolerance limits suggested by WHO/FAO.", "contents": "Cadmium uptake from feed and its distribution to food products of livestock. Distribution of cadmium (Cd) into the edible products of three species of food-producing animals was investigated during long-term dietary administration of supplemental cadmium chloride. Cows were exposed to 0.2 (control), 2.4, and 11.3 ppm Cd on whole ration basis for a period of three months followed by a three-month period on control ration. No accumulation of Cd occurred in milk, muscle, or bone at any of the time periods. A dose-related increase of Cd was observed in liver and kidney. The Cd concentration in these organs showed a gradual rise even when the animals were given control ration for three months after an initial three-month exposure to Cd; this observation suggests a mobilization and redistribution of this metal from other tissues. Dietary Cd levels in swine rations were 0.2, 2.4, and 10.1 ppm. The highest level of Cd produced a slightly reduced growth rate in swine. No accumulation of Cd was observed in muscle, bone, or brain. Liver and kidney showed a treatment and time-related increase in cadmium values at 6 and 12 weeks on experiment. During a three-month depletion phase after an initial three months of Cd administration, no further change in liver and kidney Cd levels was observed. White Leghorn chickens were treated by administering 0.3, 1.9, and 13.1 ppm Cd in their diets for up to 6 months. No accumulation of Cd occurred in eggs or bones. A slight increase of Cd level was observed in chicken muscle after six months of exposure. Liver and kidney had the highest levels of Cd, which showed a dose and time-related increase in these organs. No depletion of liver and kidney Cd was seen during seven weeks following a six-week treatment period. In all three species, the kidney Cd levels were severalfold higher than those of liver at all dietary levels. In swine, the renal cortex and medulla had similar Cd concentration in control animals but in all animals exposed to supplemental Cd, a dramatic rise in Cd levels in renal cortex was observed. Medullary Cd did not show a proportional time and dose-related increase in Cd levels, although the levels showed some increase. In kidney and liver of all three species the Cd levels showed a positive correlation with the amount of Cd-binding protein in these tissues. Induced levels of this metal-binding protein may explain accumulation and persistence of Cd-residues in these organs. In all three species, the concentrations of renal Cd-binding protein increased at a rate greater than those in liver. Although the food-producing animals may act as an effective filter of Cd in the case of an environmental increase of this metal, consumption of visceral organs from such animals may pose a hazard. This is particularly critical since the daily intake of Cd in human diet is already estimated to exceed the tolerance limits suggested by WHO/FAO."}
{"id": "PMID:488050", "title": "Deposit and mobility of cadmium in a marsh-cove ecosystem and the relation to cadmium concentration in biota.", "content": "The study reported here presents the results of an investigation of a marsh-cove ecosystem heavily contaminated by cadmium. The most contaminated aquatic sediments were dredged in 1972-73, but the resuspension of the sediments and recycle of water from the dredge spoil resulted in reestablishment of a large contaminated sediment bed with concentrations very similar to those observed before dredging. The stability of the sediment concentrations and shallow depth of the cadmium in the sediments indicate that the deposit is relatively stable in agreement with the expectations based on the water chemistry of the system. Uptake does occur in both marsh and aquatic plants and all species of animals tested. Significantly elevated concentrations are observed compared to noncontaminated areas; however, edible portions of most fish do not appear to present a hazard. Crabs appear to present the most likely source of a hazard to humans. This potential hazard is still under investigation. The dredging removed about 5.5 MT of cadmium, about one-fourth of that originally estimated to be present, but twice that amount is found to be in the cove sediments 3 to 4 years after dredging. No appreciable improvement in the ecosystem has been made, and more careful consideration should be given to the need for decontamination and the method of removal of contaminated aquatic sediments in any future case.", "contents": "Deposit and mobility of cadmium in a marsh-cove ecosystem and the relation to cadmium concentration in biota. The study reported here presents the results of an investigation of a marsh-cove ecosystem heavily contaminated by cadmium. The most contaminated aquatic sediments were dredged in 1972-73, but the resuspension of the sediments and recycle of water from the dredge spoil resulted in reestablishment of a large contaminated sediment bed with concentrations very similar to those observed before dredging. The stability of the sediment concentrations and shallow depth of the cadmium in the sediments indicate that the deposit is relatively stable in agreement with the expectations based on the water chemistry of the system. Uptake does occur in both marsh and aquatic plants and all species of animals tested. Significantly elevated concentrations are observed compared to noncontaminated areas; however, edible portions of most fish do not appear to present a hazard. Crabs appear to present the most likely source of a hazard to humans. This potential hazard is still under investigation. The dredging removed about 5.5 MT of cadmium, about one-fourth of that originally estimated to be present, but twice that amount is found to be in the cove sediments 3 to 4 years after dredging. No appreciable improvement in the ecosystem has been made, and more careful consideration should be given to the need for decontamination and the method of removal of contaminated aquatic sediments in any future case."}
{"id": "PMID:488051", "title": "Bioaccumulation of cadmium in marine organisms.", "content": "A general review of cadmium concentrations in marine organisms and studies of cadmium bioaccumulation is presented. Factors which influence cadmium concentrations, such as regional differences, seasonal fluctuations and salinity, are discussed and species which are likely to accumulate cadmium identified. Experimental studies designed to investigate the influence of some of these factors on cadmium bioaccumulation in a filter feeding bivalve mollusk, the American oyster (Crassostrea virginica), are presented. Field studies of seasonal dynamics of cadmium in oysters indicate patterns which may be correlated with seasonal physiological activity. The bioaccumulation of cadmium following input to estuarine systems by natural phenomena is observed. Cadmium concentrations in oysters collected from regions of different salinity suggest an inverse relationship between cadmium concentration and salinity. Laboratory experiments designed to investigate mechanisms of cadmium accumulation demonstrate that an inducible cadmium binding protein, similar to metallothiomein, is present in the American oyster.", "contents": "Bioaccumulation of cadmium in marine organisms. A general review of cadmium concentrations in marine organisms and studies of cadmium bioaccumulation is presented. Factors which influence cadmium concentrations, such as regional differences, seasonal fluctuations and salinity, are discussed and species which are likely to accumulate cadmium identified. Experimental studies designed to investigate the influence of some of these factors on cadmium bioaccumulation in a filter feeding bivalve mollusk, the American oyster (Crassostrea virginica), are presented. Field studies of seasonal dynamics of cadmium in oysters indicate patterns which may be correlated with seasonal physiological activity. The bioaccumulation of cadmium following input to estuarine systems by natural phenomena is observed. Cadmium concentrations in oysters collected from regions of different salinity suggest an inverse relationship between cadmium concentration and salinity. Laboratory experiments designed to investigate mechanisms of cadmium accumulation demonstrate that an inducible cadmium binding protein, similar to metallothiomein, is present in the American oyster."}
{"id": "PMID:488052", "title": "Factors influencing cadmium accumulation and its toxicity to marine organisms.", "content": "The toxicity of dissolved cadmium to a variety of marine animals has been found to be related to salinity, with decreased toxicity observed at higher salinities. Recent data from our laboratory have demonstrated that the toxicity of cadmium to estuarine shrimp and larval fish is a function of free cadmium ion concentration, which in turn is controlled by the chloride concentration of the water. As the chloride concentration (i.e., salinity of the water) increases, the concentration of free cadmium ion decreases relative to total dissolved metal, due to its complexation with chloride ions. These observations have been given further support by measurements involving the uptake of (115m)Cd by shrimp which showed that accumulation of (115m)Cd and chloride concentration also are inversely related. Experiments also have been conducted on the physiological effects of cadmium on the respiration of excised oyster gill tissue. Although tissues from oysters exposed for 14 days to 0.1 ppm total dissolved cadmium accumulated significant quantities of metal, no measurable effects on respiration rates were detected. Higher doses (0.3 and 0.6 ppm) caused both mortalities of oysters and accelerated respiration of excised oyster gill. Exposure to 0.1 ppm cadmium also caused the induction of and/or increased binding of cadmium to a specific low molecular weight protein in oysters. This protein appeared to have a detoxification function at low cadmium exposure levels, but in animals exposed to 0.6 ppm cadmium the induction mechanism apparently became saturated, allowing the excess cadmium to bind critical sites with resultant damage.", "contents": "Factors influencing cadmium accumulation and its toxicity to marine organisms. The toxicity of dissolved cadmium to a variety of marine animals has been found to be related to salinity, with decreased toxicity observed at higher salinities. Recent data from our laboratory have demonstrated that the toxicity of cadmium to estuarine shrimp and larval fish is a function of free cadmium ion concentration, which in turn is controlled by the chloride concentration of the water. As the chloride concentration (i.e., salinity of the water) increases, the concentration of free cadmium ion decreases relative to total dissolved metal, due to its complexation with chloride ions. These observations have been given further support by measurements involving the uptake of (115m)Cd by shrimp which showed that accumulation of (115m)Cd and chloride concentration also are inversely related. Experiments also have been conducted on the physiological effects of cadmium on the respiration of excised oyster gill tissue. Although tissues from oysters exposed for 14 days to 0.1 ppm total dissolved cadmium accumulated significant quantities of metal, no measurable effects on respiration rates were detected. Higher doses (0.3 and 0.6 ppm) caused both mortalities of oysters and accelerated respiration of excised oyster gill. Exposure to 0.1 ppm cadmium also caused the induction of and/or increased binding of cadmium to a specific low molecular weight protein in oysters. This protein appeared to have a detoxification function at low cadmium exposure levels, but in animals exposed to 0.6 ppm cadmium the induction mechanism apparently became saturated, allowing the excess cadmium to bind critical sites with resultant damage."}
{"id": "PMID:488053", "title": "Influence of some factors on cadmium pharmacokinetics and toxicity.", "content": "Cadmium metabolism in the young and in conditions of dietary contamination with ash from coal gasification were investigated. The experiments were performed in adult rats which received ash in the diet (5%) and/or cadmium in drinking water (100 ppm) over a period of five weeks and in sucklings whose mothers were given the same treatment throughout pregnancy and lactation. In pharmacokinetic studies, (115m)Cd was administered orally or intraperitoneally to determine the intestinal absorption, retention, and distribution. Cadmium toxicity (LD(50)) was determined in different age groups of animals treated with ash for five weeks before a single oral or intraperitoneal administration of cadmium chloride. After intraperitoneal administration, (115m)Cd body retention decreased with age and was independent of the dietary treatment. Sucklings had a higher retention in the blood, carcass, and gut than adults. After oral administration, sucklings had a much higher body retention than adults regardless of the dietary treatment of their mothers. Cadmium toxicity was also independent of the dietary treatment. Most striking was a very high oral toxicity of cadmium in sucklings. It is concluded that the young might be at a special risk at the same level of environmental cadmium exposure because of the high oral cadmium toxicity at this age which is most probably due to a high cadmium retention in the gut. It is also concluded that the mixture of elements contained in ash is not likely to influence cadmium metabolism and toxicity in conditions of dietary exposure.", "contents": "Influence of some factors on cadmium pharmacokinetics and toxicity. Cadmium metabolism in the young and in conditions of dietary contamination with ash from coal gasification were investigated. The experiments were performed in adult rats which received ash in the diet (5%) and/or cadmium in drinking water (100 ppm) over a period of five weeks and in sucklings whose mothers were given the same treatment throughout pregnancy and lactation. In pharmacokinetic studies, (115m)Cd was administered orally or intraperitoneally to determine the intestinal absorption, retention, and distribution. Cadmium toxicity (LD(50)) was determined in different age groups of animals treated with ash for five weeks before a single oral or intraperitoneal administration of cadmium chloride. After intraperitoneal administration, (115m)Cd body retention decreased with age and was independent of the dietary treatment. Sucklings had a higher retention in the blood, carcass, and gut than adults. After oral administration, sucklings had a much higher body retention than adults regardless of the dietary treatment of their mothers. Cadmium toxicity was also independent of the dietary treatment. Most striking was a very high oral toxicity of cadmium in sucklings. It is concluded that the young might be at a special risk at the same level of environmental cadmium exposure because of the high oral cadmium toxicity at this age which is most probably due to a high cadmium retention in the gut. It is also concluded that the mixture of elements contained in ash is not likely to influence cadmium metabolism and toxicity in conditions of dietary exposure."}
{"id": "PMID:488054", "title": "Some effects of oral ingestion of cadmium on zinc, copper, and iron metabolism.", "content": "Data are presented to show that ingestion of cadmium chloride by rats at low levels leads to alteration of zinc metabolism in the liver, even though the formation of metallothionein is not evident. A dose-response relationship between amount of cadmium ingested and degree of perturbation of zinc metabolism in liver was found. Oral cadmium was shown to cause emphysema and reduce pulmonary function in male rats; the effect was less severe or delayed in onset if dietary zinc concentration was high. Interference with copper and iron metabolism was shown to occur in rats given low levels of cadmium orally. Depression of copper and iron metabolism of the rat fetus was found to occur when dams received very low doses of cadmium during gestation, even though very little cadmium passed the placental barrier.", "contents": "Some effects of oral ingestion of cadmium on zinc, copper, and iron metabolism. Data are presented to show that ingestion of cadmium chloride by rats at low levels leads to alteration of zinc metabolism in the liver, even though the formation of metallothionein is not evident. A dose-response relationship between amount of cadmium ingested and degree of perturbation of zinc metabolism in liver was found. Oral cadmium was shown to cause emphysema and reduce pulmonary function in male rats; the effect was less severe or delayed in onset if dietary zinc concentration was high. Interference with copper and iron metabolism was shown to occur in rats given low levels of cadmium orally. Depression of copper and iron metabolism of the rat fetus was found to occur when dams received very low doses of cadmium during gestation, even though very little cadmium passed the placental barrier."}
{"id": "PMID:488055", "title": "Protease deficiency in plasma of patients with cystic fibrosis. Reduced reaction of 4-methylumbelliferylguanidinobenzoate with plasma of patients with cystic fibrosis.", "content": "Protease activity in plasma is assayed using 4-methylumbelliferylguanidinobenzoate. The assay is modified by carrying out the reaction in the presence and absence of benzamidine, a competitive inhibitor of trypsin-like proteases. The parameters of the assay are described in detail. Using this assay, our earlier demonstration of a deficiency of protease activity in plasma of patients with cystic fibrosis is confirmed. The activity, corrected for the nonspecific hydrolysis of 4-methylumbelliferylguanidinobenzoate by benzamidine, is expressed as nanomoles of 4-methylumbelliferone released per milliliter plasma. Under standard conditions, the activity in plasma activated with chloroform-ellagic acid was 127.2 +/- 23.1 in 7 controls, 70.4 +/- 11.7 in 11 obligate heterozygotes, and 48.7 +/- 16.6 in 12 patients with cystic fibrosis. Identical results were obtained when unactivated plasma was used. These data demonstrate that the judicious use of specific inhibitors such as benzamidine might be useful in assaying low levels of protease activity in crude systems.", "contents": "Protease deficiency in plasma of patients with cystic fibrosis. Reduced reaction of 4-methylumbelliferylguanidinobenzoate with plasma of patients with cystic fibrosis. Protease activity in plasma is assayed using 4-methylumbelliferylguanidinobenzoate. The assay is modified by carrying out the reaction in the presence and absence of benzamidine, a competitive inhibitor of trypsin-like proteases. The parameters of the assay are described in detail. Using this assay, our earlier demonstration of a deficiency of protease activity in plasma of patients with cystic fibrosis is confirmed. The activity, corrected for the nonspecific hydrolysis of 4-methylumbelliferylguanidinobenzoate by benzamidine, is expressed as nanomoles of 4-methylumbelliferone released per milliliter plasma. Under standard conditions, the activity in plasma activated with chloroform-ellagic acid was 127.2 +/- 23.1 in 7 controls, 70.4 +/- 11.7 in 11 obligate heterozygotes, and 48.7 +/- 16.6 in 12 patients with cystic fibrosis. Identical results were obtained when unactivated plasma was used. These data demonstrate that the judicious use of specific inhibitors such as benzamidine might be useful in assaying low levels of protease activity in crude systems."}
{"id": "PMID:488056", "title": "A new spectrophotometric assay for enzymes of purine metabolism. I. Determination of xanthine oxidase activity.", "content": "A new method for the determination of xanthine oxidase activity with xanthine or hypoxanthine is described. The hydrogen peroxide produced by the oxidation of the substrates is reduced by catalase in the presence of high concentrations of ethanol. The acetaldehyde formed is further oxidized by aldehyde dehydrogenase NAD or NADP-dependent. The reduction rate of the coenzymes were measured at 334 nm and utilized as indicators for the xanthine oxidase. The sensitivity of the method with xanthine as substrate can be doubled by the addition of uricase, which oxidizes uric acid to allantoin.", "contents": "A new spectrophotometric assay for enzymes of purine metabolism. I. Determination of xanthine oxidase activity. A new method for the determination of xanthine oxidase activity with xanthine or hypoxanthine is described. The hydrogen peroxide produced by the oxidation of the substrates is reduced by catalase in the presence of high concentrations of ethanol. The acetaldehyde formed is further oxidized by aldehyde dehydrogenase NAD or NADP-dependent. The reduction rate of the coenzymes were measured at 334 nm and utilized as indicators for the xanthine oxidase. The sensitivity of the method with xanthine as substrate can be doubled by the addition of uricase, which oxidizes uric acid to allantoin."}
{"id": "PMID:488057", "title": "A new spectrophotometric assay for enzymes of purine metabolism. II. Determination of guanase activity.", "content": "A new method for the determination of guanase is described. Xanthine, the product of the guanase reaction, is oxidized by xanthine oxidase, forming uric acid and hydrogen peroxide. Hydrogen peroxide is further reduced to water by catalase in the presence of ethanol. The acetaldehyde formed in this reaction step is dehydrogenated NAD or NADP dependent by aldehyde dehydrogenase. The NADH or NADPH production is measured and utilized for the calculation of the guanase activity. The sensitivity of the method can be doubled by the addition of uricase, which oxidizes uric acid to permit the formation of another mole of hydrogen peroxide.", "contents": "A new spectrophotometric assay for enzymes of purine metabolism. II. Determination of guanase activity. A new method for the determination of guanase is described. Xanthine, the product of the guanase reaction, is oxidized by xanthine oxidase, forming uric acid and hydrogen peroxide. Hydrogen peroxide is further reduced to water by catalase in the presence of ethanol. The acetaldehyde formed in this reaction step is dehydrogenated NAD or NADP dependent by aldehyde dehydrogenase. The NADH or NADPH production is measured and utilized for the calculation of the guanase activity. The sensitivity of the method can be doubled by the addition of uricase, which oxidizes uric acid to permit the formation of another mole of hydrogen peroxide."}
{"id": "PMID:488058", "title": "Cystathionase deficiency: the effect of cofactor on the stability of normal and abnormal enzyme from lymphoid cell lines.", "content": "Heating at 70 degrees C with and without added pyridoxal phosphate (PLP) had strikingly different effects on cystathionase protein from normal long-term lymphoid cell lines and on the enzymes from cells derived from patients with vitamin-B6-responsive cystathioninuria. PLP added to extracts of normal cells afforded complete protection against heat inactivation, whereas inactivation of the cystathionase protein in extracts obtained from two cystathioninuric lines was greater in the presence of PLP than in its absence.", "contents": "Cystathionase deficiency: the effect of cofactor on the stability of normal and abnormal enzyme from lymphoid cell lines. Heating at 70 degrees C with and without added pyridoxal phosphate (PLP) had strikingly different effects on cystathionase protein from normal long-term lymphoid cell lines and on the enzymes from cells derived from patients with vitamin-B6-responsive cystathioninuria. PLP added to extracts of normal cells afforded complete protection against heat inactivation, whereas inactivation of the cystathionase protein in extracts obtained from two cystathioninuric lines was greater in the presence of PLP than in its absence."}
{"id": "PMID:488059", "title": "Activity of arylamidases in serum during normal pregnancy.", "content": "Activity of arylamidases toward 6 beta-naphthylamides of L-amino acids was studied in the blood serum from 110 healthy women between the 6th and 36th weeks of pregnancy. A regular increase in the enzymatic activity was demonstrated toward all substrates under examination, particularly in the presence of alanyl-, leucyl- and lysyl-beta-naphthylamides, most pronounced in the last trimester of gestation. A correlation between oxytocinase and arylamidases activity was demonstrated, suggesting a possibility of using the enzymatic measurement as a diagnostic test in the normal course of pregnancy.", "contents": "Activity of arylamidases in serum during normal pregnancy. Activity of arylamidases toward 6 beta-naphthylamides of L-amino acids was studied in the blood serum from 110 healthy women between the 6th and 36th weeks of pregnancy. A regular increase in the enzymatic activity was demonstrated toward all substrates under examination, particularly in the presence of alanyl-, leucyl- and lysyl-beta-naphthylamides, most pronounced in the last trimester of gestation. A correlation between oxytocinase and arylamidases activity was demonstrated, suggesting a possibility of using the enzymatic measurement as a diagnostic test in the normal course of pregnancy."}
{"id": "PMID:488060", "title": "Differential heat sensitivity of human creatine kinase isoenzymes.", "content": "Creatine kinase isoenzymes (MM, MB and BB) were isolated from human tissue by ion-exchange chromatography. The B subunit was found to be more heat sensitive than the M subunit. BB and MB isoenzymes respond similarly to heat inactivation. Our results are in contrast with the body temperature inactivation of the brain isoenzyme reported by Lindsey and Diamond.", "contents": "Differential heat sensitivity of human creatine kinase isoenzymes. Creatine kinase isoenzymes (MM, MB and BB) were isolated from human tissue by ion-exchange chromatography. The B subunit was found to be more heat sensitive than the M subunit. BB and MB isoenzymes respond similarly to heat inactivation. Our results are in contrast with the body temperature inactivation of the brain isoenzyme reported by Lindsey and Diamond."}
{"id": "PMID:488061", "title": "Endoscopic observations on the deglutition reflex in the horse.", "content": "Cine-endoscopy was used to study the deglutition reflex in 2 apparently normal horses. Closure of the nasopharyngeal sphincter was brought about by a lowering of the pharyngeal roof and an elevation of the soft palate caudal to the pharyngeal ostia of the auditory tubes. The medial cartilages of the ostia were not directly involved in bringing about closure of the sphincter. It is postulated that the opening of the ostia of the auditory tubes is brought about during deglutition by the combined action of the palatopharyngeus, pterygopharyngeus and tensor veli palatini muscles.", "contents": "Endoscopic observations on the deglutition reflex in the horse. Cine-endoscopy was used to study the deglutition reflex in 2 apparently normal horses. Closure of the nasopharyngeal sphincter was brought about by a lowering of the pharyngeal roof and an elevation of the soft palate caudal to the pharyngeal ostia of the auditory tubes. The medial cartilages of the ostia were not directly involved in bringing about closure of the sphincter. It is postulated that the opening of the ostia of the auditory tubes is brought about during deglutition by the combined action of the palatopharyngeus, pterygopharyngeus and tensor veli palatini muscles."}
{"id": "PMID:488062", "title": "Observations on the mechanism of functional obstruction of the nasopharyngeal airway in the horse.", "content": "Fibreoptic endoscopy was used to study the movements of the larynx and pharynx during nasal occlusion in 10 horses, which showed signs consistent with functional pharyngeal obstruction (FPO) on exercise. Cine-endoscopic films were made on 3 such horses. Consideration of the anatomy of the region indicates that FPO may best be regarded as a subluxation of the nasopharyngeal and larygneal airways and it was found that a constant component of the movements which brought about this subluxation was a marked caudal retraction of the larynx. It is suggested that this caudal retraction of the larynx occurred as a result of the action of th sternothyrohyoid and omohyoid muscles and that removal of a portion of these muscles might prevent subsequent airway subluxation in horses prone to FPO on exercise.", "contents": "Observations on the mechanism of functional obstruction of the nasopharyngeal airway in the horse. Fibreoptic endoscopy was used to study the movements of the larynx and pharynx during nasal occlusion in 10 horses, which showed signs consistent with functional pharyngeal obstruction (FPO) on exercise. Cine-endoscopic films were made on 3 such horses. Consideration of the anatomy of the region indicates that FPO may best be regarded as a subluxation of the nasopharyngeal and larygneal airways and it was found that a constant component of the movements which brought about this subluxation was a marked caudal retraction of the larynx. It is suggested that this caudal retraction of the larynx occurred as a result of the action of th sternothyrohyoid and omohyoid muscles and that removal of a portion of these muscles might prevent subsequent airway subluxation in horses prone to FPO on exercise."}
{"id": "PMID:488063", "title": "Fluoroscopic investigation of pharyngeal function in the horse.", "content": "Videofluoroscopy was used to study the deglutition reflexin 2 horses believed, on the basis of endoscopic and clinical examination, to have normal pharyngeal and laryngeal function. The reflex was found to be the same as that described in man and in the rabbit. A feature of deglutition in the horse was the temporary increase in size of the auditory tube diverticuli (gluttural pouches) as a result of contraction of the pharyngeal muscles.", "contents": "Fluoroscopic investigation of pharyngeal function in the horse. Videofluoroscopy was used to study the deglutition reflexin 2 horses believed, on the basis of endoscopic and clinical examination, to have normal pharyngeal and laryngeal function. The reflex was found to be the same as that described in man and in the rabbit. A feature of deglutition in the horse was the temporary increase in size of the auditory tube diverticuli (gluttural pouches) as a result of contraction of the pharyngeal muscles."}
{"id": "PMID:488064", "title": "The effect of laryngoplasty on pharyngeal function in the horse.", "content": "Using fluoroscopic techniques and videotape recordings, a study of normal deglutition was made in 2 ponies. Paryngeal function was studied at intervals after laryngoplasty on 1 animal and after a sham technique on the other. Two clinical cases of laryngeal paralysis, which had been treated by laryngoplasty, were also examined. In the 2 experimental ponies, liquid food passed into the lower respiratory tract post-operatively. One of the clinical cases appeared to swallow solid food normally, but some food material entered the larynx of the other horse. It is suggested that pharyngeal dysfunction, as well as over-abduction of the arytenoid cartilage, might be involved in causing the chronic post-operative cough which the experiment was designed to investigate.", "contents": "The effect of laryngoplasty on pharyngeal function in the horse. Using fluoroscopic techniques and videotape recordings, a study of normal deglutition was made in 2 ponies. Paryngeal function was studied at intervals after laryngoplasty on 1 animal and after a sham technique on the other. Two clinical cases of laryngeal paralysis, which had been treated by laryngoplasty, were also examined. In the 2 experimental ponies, liquid food passed into the lower respiratory tract post-operatively. One of the clinical cases appeared to swallow solid food normally, but some food material entered the larynx of the other horse. It is suggested that pharyngeal dysfunction, as well as over-abduction of the arytenoid cartilage, might be involved in causing the chronic post-operative cough which the experiment was designed to investigate."}
{"id": "PMID:488065", "title": "Chronic obstructive pulmonary disease (COPD): factors influencing the occurrence.", "content": "Breed, age, weight, type of work performed, seasonal onset, poor ventilation and exposure to moulds in the habitat were investigated in relation to the occurrence of chronic obstructive pulmonary disease (COPD). COPD was most commonly detected in showjumping and hacking horses. The older a horse, the more likely it was to become affected although most were 6 to 10 years of age. Of the horses in this sample of the population, which was not a random one, thoroughbred horses were affected least and ponies most often. The high incidence in ponies was related to their more frequent exposure to poor quality fodder and bedding. Most horses are exposed to the hazard of moulds, but more affected horses were so exposed than those not affected with COPD. Poor ventilation of the stable increased the chance of a horse becoming affected. Sex, body weight and season of onset of coughing had no influence on the occurrence of the disease.", "contents": "Chronic obstructive pulmonary disease (COPD): factors influencing the occurrence. Breed, age, weight, type of work performed, seasonal onset, poor ventilation and exposure to moulds in the habitat were investigated in relation to the occurrence of chronic obstructive pulmonary disease (COPD). COPD was most commonly detected in showjumping and hacking horses. The older a horse, the more likely it was to become affected although most were 6 to 10 years of age. Of the horses in this sample of the population, which was not a random one, thoroughbred horses were affected least and ponies most often. The high incidence in ponies was related to their more frequent exposure to poor quality fodder and bedding. Most horses are exposed to the hazard of moulds, but more affected horses were so exposed than those not affected with COPD. Poor ventilation of the stable increased the chance of a horse becoming affected. Sex, body weight and season of onset of coughing had no influence on the occurrence of the disease."}
{"id": "PMID:488066", "title": "Phonocardiography in the horse: 2. The relationship of the external phonocardiogram to intracardiac pressure and sound.", "content": "The paper describes observations during individual cardiac cycles on the changing pressures and sounds recorded from the various chambers of the heart and aorta, related to phonocardiograms from an external microphone hand-held over the cardiac area. The majority of the first sound appears to arise from the left side of the heart after the AV valves have closed and the second sound is a consequence of sudden deceleration of blood against already closed semilunar valves. Systolic murmurs at the aortic root may be benign from increased flow and turbulence at the peak of ejection but these may not be recorded externally.", "contents": "Phonocardiography in the horse: 2. The relationship of the external phonocardiogram to intracardiac pressure and sound. The paper describes observations during individual cardiac cycles on the changing pressures and sounds recorded from the various chambers of the heart and aorta, related to phonocardiograms from an external microphone hand-held over the cardiac area. The majority of the first sound appears to arise from the left side of the heart after the AV valves have closed and the second sound is a consequence of sudden deceleration of blood against already closed semilunar valves. Systolic murmurs at the aortic root may be benign from increased flow and turbulence at the peak of ejection but these may not be recorded externally."}
{"id": "PMID:488067", "title": "A preliminary report on the use of warfarin in the treatment of navicular disease.", "content": "Twenty horses suffering from navicular disease were treated with warfarin given orally. The dosage was to effect, to give a 2 to 4 second prolongation of the one stage prothrombin time (OSPT). Dosage was initially at a rate of 0.018 mg/kg, changing the dose by amounts of 20 per cent until the required dose was achieved. Final dose rates varied from 0.012 mg/kg to 0.75 mg/kg. All the cases treated received warfarin daily throughout the trial. Seventeen of the animals became sound and the remaining 3 showed a marked improvement in their gait. The mean of the ages of the horses was 7.5 years, the mean of the periods of lameness 9 months and the mean of the time taken to recovery one treatment was commenced was 7 weeks.", "contents": "A preliminary report on the use of warfarin in the treatment of navicular disease. Twenty horses suffering from navicular disease were treated with warfarin given orally. The dosage was to effect, to give a 2 to 4 second prolongation of the one stage prothrombin time (OSPT). Dosage was initially at a rate of 0.018 mg/kg, changing the dose by amounts of 20 per cent until the required dose was achieved. Final dose rates varied from 0.012 mg/kg to 0.75 mg/kg. All the cases treated received warfarin daily throughout the trial. Seventeen of the animals became sound and the remaining 3 showed a marked improvement in their gait. The mean of the ages of the horses was 7.5 years, the mean of the periods of lameness 9 months and the mean of the time taken to recovery one treatment was commenced was 7 weeks."}
{"id": "PMID:488068", "title": "Indirect measurement of mean blood pressure in the normotensive and hypotensive horse.", "content": "Indirect mean arterial pressure was obtained from the tails of 78 anaesthetised and unanaesthetised normotensive and hypotensive horses. Data were obtained to determine the optimum cuff width in relation to tail circumference. A cuff that is too wide under-estimates and a cuff that is too narrow over-estimates blood pressure. With the optimum cuff width, which is slightly in excess of about one-fifth of the tail circumference, indirect mean blood pressure was obtained consistently in normotensive and hypotensive animals with mean blood pressures of 40 mmHg and frequently in animals with pressure as low as 30 mmHg.", "contents": "Indirect measurement of mean blood pressure in the normotensive and hypotensive horse. Indirect mean arterial pressure was obtained from the tails of 78 anaesthetised and unanaesthetised normotensive and hypotensive horses. Data were obtained to determine the optimum cuff width in relation to tail circumference. A cuff that is too wide under-estimates and a cuff that is too narrow over-estimates blood pressure. With the optimum cuff width, which is slightly in excess of about one-fifth of the tail circumference, indirect mean blood pressure was obtained consistently in normotensive and hypotensive animals with mean blood pressures of 40 mmHg and frequently in animals with pressure as low as 30 mmHg."}
{"id": "PMID:488081", "title": "Analysis of rapid oscillations of glucose and free fatty acids in plasma.", "content": "The authors analyzed rapid oscillations of blood sugar (GL) and free fatty acid levels (FFA) in serum of healthy subjects. They investigated a series of blood samples taken under conditions of absolute rest from the cubital vein at 15-s intervals for a period of 6 min. In addition to common statistical parameters, they calculated the course of autocorrelation and cross-correlation functions and periodograms. The magnitude of oscillations is significantly higher than the error of the biochemical methods. In some sequences periodicities were detected which were statistically significant in 23.8% of GL and in 38.1% of FFA. 24-point series of GL collected in parallel from both arms correlate in 36.3% positively, in 27.3% negatively, and in 36.4% they do not correlate. Series of FFA and GL collected simultaneously from one site correlate mutaually in almost all instances either positively or negatively, frequently with a time shift. The oscillations may be due to (a) feedback regulations of the levels of the two metabolites, (b) permanent mutual interaction between the FFA and glucose level and (c) an uneven concentration of the two metabolites in different parts of the circulation. The above factors may combine, and the list of possible factors may not be complete.", "contents": "Analysis of rapid oscillations of glucose and free fatty acids in plasma. The authors analyzed rapid oscillations of blood sugar (GL) and free fatty acid levels (FFA) in serum of healthy subjects. They investigated a series of blood samples taken under conditions of absolute rest from the cubital vein at 15-s intervals for a period of 6 min. In addition to common statistical parameters, they calculated the course of autocorrelation and cross-correlation functions and periodograms. The magnitude of oscillations is significantly higher than the error of the biochemical methods. In some sequences periodicities were detected which were statistically significant in 23.8% of GL and in 38.1% of FFA. 24-point series of GL collected in parallel from both arms correlate in 36.3% positively, in 27.3% negatively, and in 36.4% they do not correlate. Series of FFA and GL collected simultaneously from one site correlate mutaually in almost all instances either positively or negatively, frequently with a time shift. The oscillations may be due to (a) feedback regulations of the levels of the two metabolites, (b) permanent mutual interaction between the FFA and glucose level and (c) an uneven concentration of the two metabolites in different parts of the circulation. The above factors may combine, and the list of possible factors may not be complete."}
{"id": "PMID:488082", "title": "Perception of effort in isometric and dynamic muscular contraction.", "content": "The perception of muscular effort was studied using estimation and production methods in the adductor pollicis and quadriceps. A psychometric scale (percentage magnitude) was used. Static contractions were studied in the adductor pollicis, and both dynamic (isokinetic) and static contractions were studied in quadriceps. Linear and logarithmic equations were fitted for the perceived effort as a percentage of the maximum in relation to the produced percentage maximal force or torque. The logarithmic exponent was around or above 1.0. No significant difference was found between mean exponent and intercept values for the adductor pollicis and the quadriceps, or when estimated or produced values for the two muscles were compared. There was no difference in the same subjects between the equations for static and dynamic contractions with low angular velocity of the quadriceps.", "contents": "Perception of effort in isometric and dynamic muscular contraction. The perception of muscular effort was studied using estimation and production methods in the adductor pollicis and quadriceps. A psychometric scale (percentage magnitude) was used. Static contractions were studied in the adductor pollicis, and both dynamic (isokinetic) and static contractions were studied in quadriceps. Linear and logarithmic equations were fitted for the perceived effort as a percentage of the maximum in relation to the produced percentage maximal force or torque. The logarithmic exponent was around or above 1.0. No significant difference was found between mean exponent and intercept values for the adductor pollicis and the quadriceps, or when estimated or produced values for the two muscles were compared. There was no difference in the same subjects between the equations for static and dynamic contractions with low angular velocity of the quadriceps."}
{"id": "PMID:488083", "title": "Plasma noradrenaline response to sustained handgrip in patients with essential hypertension.", "content": "The responses of plasma noradrenaline, arterial blood pressure, and heart rate to sustained handgrip at 30% maximal voluntary contraction were studied in untreated patients with essential hypertension and in healthy subjects of comparable age. There were no significant differences between these two groups in the intensity and duration of handgrip. Increases in heart rate and blood pressure induced by the effort were similar in hypertensive patients and normotensive control subjects, whereas the absolute levels of blood pressure were considerably higher in the patients. In the first 1-2 min of exercise the increases in plasma noradrenaline concentration were similar in both groups. Subsequently, plasma noradrenaline concentration tended to plateau in hypertensive patients while in control subjects it continued to increase. The elevation of plasma noradrenaline in the last minute of effort was, therefore, significantly smaller in hypertensive patients than in the control group.", "contents": "Plasma noradrenaline response to sustained handgrip in patients with essential hypertension. The responses of plasma noradrenaline, arterial blood pressure, and heart rate to sustained handgrip at 30% maximal voluntary contraction were studied in untreated patients with essential hypertension and in healthy subjects of comparable age. There were no significant differences between these two groups in the intensity and duration of handgrip. Increases in heart rate and blood pressure induced by the effort were similar in hypertensive patients and normotensive control subjects, whereas the absolute levels of blood pressure were considerably higher in the patients. In the first 1-2 min of exercise the increases in plasma noradrenaline concentration were similar in both groups. Subsequently, plasma noradrenaline concentration tended to plateau in hypertensive patients while in control subjects it continued to increase. The elevation of plasma noradrenaline in the last minute of effort was, therefore, significantly smaller in hypertensive patients than in the control group."}
{"id": "PMID:488084", "title": "Cardiovascular response to interval and continuous training in women.", "content": "Three groups of five women (age = 18--25 years) participated in a 12-week training program. Cardiovascular responses up to 85% VO2 max to interval (ITG) and continuous (CTG) training were studied in two groups, before training and after 4, 8, and 12 weeks of training four times per week. A control group was assessed before and after 6 and 12 weeks. Both exercise groups demonstrated significant increases in Cao2--Cvo2 after 8 weeks with only slight further increases after 12 weeks (CTG = 8.9%, ITG = 20.0% at 85% VO2 max). No significant changes were noted in either group in SV (+ 5 ml ITG, + 9 ml CTG) or in their Qc. These results indicated that, in response to high intensity training, women may demonstrate similar cardiovascular adaptations to training as have been observed for men.", "contents": "Cardiovascular response to interval and continuous training in women. Three groups of five women (age = 18--25 years) participated in a 12-week training program. Cardiovascular responses up to 85% VO2 max to interval (ITG) and continuous (CTG) training were studied in two groups, before training and after 4, 8, and 12 weeks of training four times per week. A control group was assessed before and after 6 and 12 weeks. Both exercise groups demonstrated significant increases in Cao2--Cvo2 after 8 weeks with only slight further increases after 12 weeks (CTG = 8.9%, ITG = 20.0% at 85% VO2 max). No significant changes were noted in either group in SV (+ 5 ml ITG, + 9 ml CTG) or in their Qc. These results indicated that, in response to high intensity training, women may demonstrate similar cardiovascular adaptations to training as have been observed for men."}
{"id": "PMID:488085", "title": "Physical training under the influence of beta blockade in rats: effect on adrenergic responses.", "content": "Rats were trained by daily swimming or running exercises with and without daily propranolol injections. Both training methods resulted in cardiac enlargement, but only swimming exercise caused hypertrophy of the brown adipose tissue. These changes were antagonized by beta blockade. The size of the adrenals reflected the stress of the treatments, but other known stress parameters, such as the size of the thymus or sexual organs dit not. Only swimming training without beta blockade sensitized the rats to the calorigenic action of noradrenaline. The cooling rate of the rats in water, when taking into account the insulative capacity of the body, was decreased in swimming-trained as well as in propranolol-treated rats but increased in running-trained rats. The latter two changes may be due to circulatory alterations, while the delayed body cooling in swimming-trained rats probably results from increased heat production capacity. Training-induced resting bradycardia and enhanced tachycardic response to isoprenaline were observable only in the animal groups trained without beta blockade. The pressor response to noradrenaline tended to be higher in the trained groups and the propranolol-treated group than in the controls and was smaller in the animal groups trained under the influence of beta blockade. On the other hand, the hypotonic response to isoprenaline was smaller in the propranolol-treated and running-trained animals. The results emphasize the importance of the sympathetic nervous system in the adaptation of an organism to physical training.", "contents": "Physical training under the influence of beta blockade in rats: effect on adrenergic responses. Rats were trained by daily swimming or running exercises with and without daily propranolol injections. Both training methods resulted in cardiac enlargement, but only swimming exercise caused hypertrophy of the brown adipose tissue. These changes were antagonized by beta blockade. The size of the adrenals reflected the stress of the treatments, but other known stress parameters, such as the size of the thymus or sexual organs dit not. Only swimming training without beta blockade sensitized the rats to the calorigenic action of noradrenaline. The cooling rate of the rats in water, when taking into account the insulative capacity of the body, was decreased in swimming-trained as well as in propranolol-treated rats but increased in running-trained rats. The latter two changes may be due to circulatory alterations, while the delayed body cooling in swimming-trained rats probably results from increased heat production capacity. Training-induced resting bradycardia and enhanced tachycardic response to isoprenaline were observable only in the animal groups trained without beta blockade. The pressor response to noradrenaline tended to be higher in the trained groups and the propranolol-treated group than in the controls and was smaller in the animal groups trained under the influence of beta blockade. On the other hand, the hypotonic response to isoprenaline was smaller in the propranolol-treated and running-trained animals. The results emphasize the importance of the sympathetic nervous system in the adaptation of an organism to physical training."}
{"id": "PMID:488086", "title": "Effect of training on hormonal responses to exercise in competitive swimmers.", "content": "The effects of 9 weeks of training on responses of plasma hormones to swimming were studied in eight competitive swimmers who had not trained for several months. Two types of swimming tests were used: (1) 200 yd, a high intensity, exhausting type of exercise in which maximal effort was required both before and after training, and (2) 1000 yd, a pace type of exercise in which subjects swam as fast as possible prior to training and at the same rate after training. Plasma levels of glucagon increased and of insulin decreased during 1000 yd of swimming, but were not altered by 200 yd of swimming. No training effects were apparent in responses of plasma insulin and glucagon to these shortterm, high intensity exercise tests. During the 1000 yd swim, plasma adrenaline was 0.8 ng/ml before vs. 0.1 ng/ml after training. Plasma noradrenaline response decreased from 3.4 to 1.2 ng/ml as a result of training. In the 200 yd swim, adrenaline, but not noradrenaline, was lower after training.", "contents": "Effect of training on hormonal responses to exercise in competitive swimmers. The effects of 9 weeks of training on responses of plasma hormones to swimming were studied in eight competitive swimmers who had not trained for several months. Two types of swimming tests were used: (1) 200 yd, a high intensity, exhausting type of exercise in which maximal effort was required both before and after training, and (2) 1000 yd, a pace type of exercise in which subjects swam as fast as possible prior to training and at the same rate after training. Plasma levels of glucagon increased and of insulin decreased during 1000 yd of swimming, but were not altered by 200 yd of swimming. No training effects were apparent in responses of plasma insulin and glucagon to these shortterm, high intensity exercise tests. During the 1000 yd swim, plasma adrenaline was 0.8 ng/ml before vs. 0.1 ng/ml after training. Plasma noradrenaline response decreased from 3.4 to 1.2 ng/ml as a result of training. In the 200 yd swim, adrenaline, but not noradrenaline, was lower after training."}
{"id": "PMID:488087", "title": "Transient-phase studies on the creatine kinase reaction. The analysis of a reaction pathway with three intermediates.", "content": "1. The initial formation of creatine phosphate by creatine kinase was studied in the millisecond range and the effect of temperature on the transient and steady-state phases exploited. 2. At 25 degrees C and 35 degrees C there was no transient phase. This is in agreement with the results of Gutfreund [Engelborghs, Y., Marsh, A., and Gutfreund, H. (1975) Biochem. J. 151, 47--50]. 3. At 4 degrees C the time course of creatine phosphate formation was complex and consisted of three transient phases: a lag phase, a burst phase and a steady-state phase. Based on this result a reaction scheme for creatine kinase which includes three intermediates was proposed. Despite the completeness of the time course, the extraction of estimates for the rate constants was difficult and computer simulation and iterative methods had to be resorted to. 4. Attempts were made to provide evidence for the complex enzyme.ADP.metaphosphate.creatine on the creatine kinase reaction pathway [cf. Milner-White, E.J. and Watts, D.C. (1971) Biochem. J. 122, 727--740]. Under the conditions used these attempts were unsuccessful at times down to 2.5 ms, at 4 degrees C or 35 degrees C.", "contents": "Transient-phase studies on the creatine kinase reaction. The analysis of a reaction pathway with three intermediates. 1. The initial formation of creatine phosphate by creatine kinase was studied in the millisecond range and the effect of temperature on the transient and steady-state phases exploited. 2. At 25 degrees C and 35 degrees C there was no transient phase. This is in agreement with the results of Gutfreund [Engelborghs, Y., Marsh, A., and Gutfreund, H. (1975) Biochem. J. 151, 47--50]. 3. At 4 degrees C the time course of creatine phosphate formation was complex and consisted of three transient phases: a lag phase, a burst phase and a steady-state phase. Based on this result a reaction scheme for creatine kinase which includes three intermediates was proposed. Despite the completeness of the time course, the extraction of estimates for the rate constants was difficult and computer simulation and iterative methods had to be resorted to. 4. Attempts were made to provide evidence for the complex enzyme.ADP.metaphosphate.creatine on the creatine kinase reaction pathway [cf. Milner-White, E.J. and Watts, D.C. (1971) Biochem. J. 122, 727--740]. Under the conditions used these attempts were unsuccessful at times down to 2.5 ms, at 4 degrees C or 35 degrees C."}
{"id": "PMID:488088", "title": "[Characterisation of a new endopeptidase from sporulating Bacillus sphaericus which is specific for the gamma-D-glutamyl-L-lysine and gamma-D-glutamyl-(L)meso-diaminopimelate linkages of peptidoglycan substrates (author's transl)].", "content": "A new peptidase which splits substrates related to the peptidic chains of peptidoglycans was found in the cell cytoplasm of sporulating Bacillus sphaericus. This is a gamma-D-glutamyl-L-diaminoacid endopeptidase (endopeptidase II). It was shown to have substrate requirements different from those of the previously described gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase (endopeptidase I). The substrates for endopeptidase II are peptides of the general type: formula: (see text). Unsubstituted N-terminal L-alanine was a strict requirement for endopeptidase II activity. Specific activities were variable with the nature and the substitution of the diaminoacid C-terminal groups. The role of endopeptidase II in the biosynthesis of the spore cortex is discussed.", "contents": "[Characterisation of a new endopeptidase from sporulating Bacillus sphaericus which is specific for the gamma-D-glutamyl-L-lysine and gamma-D-glutamyl-(L)meso-diaminopimelate linkages of peptidoglycan substrates (author's transl)]. A new peptidase which splits substrates related to the peptidic chains of peptidoglycans was found in the cell cytoplasm of sporulating Bacillus sphaericus. This is a gamma-D-glutamyl-L-diaminoacid endopeptidase (endopeptidase II). It was shown to have substrate requirements different from those of the previously described gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase (endopeptidase I). The substrates for endopeptidase II are peptides of the general type: formula: (see text). Unsubstituted N-terminal L-alanine was a strict requirement for endopeptidase II activity. Specific activities were variable with the nature and the substitution of the diaminoacid C-terminal groups. The role of endopeptidase II in the biosynthesis of the spore cortex is discussed."}
{"id": "PMID:488090", "title": "Fructose-1,6-bisphosphate aldolase from rabbit liver. Reaction mechanism and physiological function.", "content": "Liver and muscle aldolase display similar reaction mechanisms. Both the enzymes, by reacting with dihydroxyacetone phosphate, form an acid-labile intermediate which is in rapid equilibrium with an eneamine intermediate. Differences are found in the equilibrium concentration of the acid-labile intermediate, which represents approximately 25% of the total intermediates in the liver (this paper) and 60% in the muscle enzyme [E. Grazi and G. Trombetta, Biochem. J. 175, 361 (1978)] and in the rate of formation of the eneamine intermediate which is much slower in the liver enzyme. Furthermore, with liver aldolase, the rate by which the C-3H bond of dihydroxyacetone phosphate is cleaved is increased by 60 times in the presence of glyceraldehyde 3-phosphate. This, mechanistically, indicates that glyceraldehyde 3-phosphate is bound to the enzyme before the formation of the eneamine from dihydroxyacetone phosphate, and, physiologically, that in liever aldolase the gluconeogenetic activity is favoured over the glycolytic activity.", "contents": "Fructose-1,6-bisphosphate aldolase from rabbit liver. Reaction mechanism and physiological function. Liver and muscle aldolase display similar reaction mechanisms. Both the enzymes, by reacting with dihydroxyacetone phosphate, form an acid-labile intermediate which is in rapid equilibrium with an eneamine intermediate. Differences are found in the equilibrium concentration of the acid-labile intermediate, which represents approximately 25% of the total intermediates in the liver (this paper) and 60% in the muscle enzyme [E. Grazi and G. Trombetta, Biochem. J. 175, 361 (1978)] and in the rate of formation of the eneamine intermediate which is much slower in the liver enzyme. Furthermore, with liver aldolase, the rate by which the C-3H bond of dihydroxyacetone phosphate is cleaved is increased by 60 times in the presence of glyceraldehyde 3-phosphate. This, mechanistically, indicates that glyceraldehyde 3-phosphate is bound to the enzyme before the formation of the eneamine from dihydroxyacetone phosphate, and, physiologically, that in liever aldolase the gluconeogenetic activity is favoured over the glycolytic activity."}
{"id": "PMID:488092", "title": "Carbon magnetic resonance studies of the self-aggregation of calf thymus histones.", "content": "Self-aggregation of calf thymus histones H2A and H4 was studied by means of 13C magnetic resonance. Analyses of the changes in the intensities of several side-chain resonances, induced by added NaCl, confirm that carbon magnetic resonance can be used to monitor aggregation phenomena more accurately than other physicochemical methods. In particular the validity of an original computer-based method to treat 13C intensities is confirmed. The details of these aggregation phenomena are critically discussed in the light of a simple experiment on a 13C-enriched sample of H2B.", "contents": "Carbon magnetic resonance studies of the self-aggregation of calf thymus histones. Self-aggregation of calf thymus histones H2A and H4 was studied by means of 13C magnetic resonance. Analyses of the changes in the intensities of several side-chain resonances, induced by added NaCl, confirm that carbon magnetic resonance can be used to monitor aggregation phenomena more accurately than other physicochemical methods. In particular the validity of an original computer-based method to treat 13C intensities is confirmed. The details of these aggregation phenomena are critically discussed in the light of a simple experiment on a 13C-enriched sample of H2B."}
{"id": "PMID:488093", "title": "Rearrangement of chromatin structure induced by increasing ionic strength and temperature.", "content": "Native rat liver chromatin fragments exposed to 600 mM NaCl at 37 degrees C for 45 min exhibit substantial modification of their original (approximately 200 base pairs) repeating subunit structure: a new repeat of 140 base pairs, superimposed on a high background, is observed after micrococcal nuclease digestion. The same material appears, in the electron microscope, as clusters of tightly packed beads connected by stretches of 'free' DNA. These modifications are not observed when the native chromatin is incubated at 37 degrees C at NaCl concentrations up to 400 mM. When native rat liver chromatin depleted of histone H1 by tRNA extraction is exposed to ionic strengths up to 600 mM NaCl at 4 degrees C, almost no modifications of the original native repeating structure are observed. However, when the incubation is carried out at 37 degrees C in 150, 300 or 400 mM NaCl, rearrangements of the native structure occur as indicated by micrococcal nuclease digestion and electron microscopic studies. Incubation of H1-depleted chromatin at 600 mM NaCl for 45 min at 37 degrees C induces, as for the native chromatin, a complete rearrangement characterized by the appearance of a 140-base-pair repeat superimposed on a high background upon digestion by micrococcal nuclease. It is suggested that these rearrangements are mediated by hydrophobic interactions between the histone cores and are prevented at ionic strengths lower than 500 mM by the presence of histone H1.", "contents": "Rearrangement of chromatin structure induced by increasing ionic strength and temperature. Native rat liver chromatin fragments exposed to 600 mM NaCl at 37 degrees C for 45 min exhibit substantial modification of their original (approximately 200 base pairs) repeating subunit structure: a new repeat of 140 base pairs, superimposed on a high background, is observed after micrococcal nuclease digestion. The same material appears, in the electron microscope, as clusters of tightly packed beads connected by stretches of 'free' DNA. These modifications are not observed when the native chromatin is incubated at 37 degrees C at NaCl concentrations up to 400 mM. When native rat liver chromatin depleted of histone H1 by tRNA extraction is exposed to ionic strengths up to 600 mM NaCl at 4 degrees C, almost no modifications of the original native repeating structure are observed. However, when the incubation is carried out at 37 degrees C in 150, 300 or 400 mM NaCl, rearrangements of the native structure occur as indicated by micrococcal nuclease digestion and electron microscopic studies. Incubation of H1-depleted chromatin at 600 mM NaCl for 45 min at 37 degrees C induces, as for the native chromatin, a complete rearrangement characterized by the appearance of a 140-base-pair repeat superimposed on a high background upon digestion by micrococcal nuclease. It is suggested that these rearrangements are mediated by hydrophobic interactions between the histone cores and are prevented at ionic strengths lower than 500 mM by the presence of histone H1."}
{"id": "PMID:488094", "title": "A restriction map of cauliflower mosaic virus DNA (strain PV 147). Mapping of the cleavage sites of HhaI, SacI, AvaI, PvuII, PstI, XbaI, EcoRI, Bg/II, HincII, HpaII and HindII + III.", "content": "The virion-extracted DNA (Mr5 x 10(6)) of cauliflower mosaic virus (CaMV) has three single-stranded interruptions. The mapping of this DNA using eleven restriction endonucleases (HhaI, SacI, AvaI, PvuII, PstI, XbaI, EcoRI, Bg/II, HincII, HpaII and HindII + III) is reported here. The existence of the three single-stranded breaks complicates the identification and the molecular weight determination of fragments produced by HpaII, HindIII and HindII + III. Indeed the electrophoretic mobility of some fragments in which a single-stranded discontinuity is located is modified, and the fluorescence of ethidium bromide complexed with these fragments is reduced as compared to that observed for the other fragments existing in a molar ratio. These drawbacks were overcome by performing experiments of nick-translation of CaMV DNA with Escherichia coli DNA polymerase I. FRom the data it follows that the CaMV DNA molecule bears bears 1 site for HhaI and SacI, 2 for AvaI and PvuII, 3 for PstI, 4 for XbaI, 5 for EcoRI, 6 for Bg/II and HincII, 11 for HpaII and 15 for HindII + III. The corresponding fragments have all been ordered and precisely located providing a suitable map for further investigations connected with the study of the fine structure and the function of the CaMV genome.", "contents": "A restriction map of cauliflower mosaic virus DNA (strain PV 147). Mapping of the cleavage sites of HhaI, SacI, AvaI, PvuII, PstI, XbaI, EcoRI, Bg/II, HincII, HpaII and HindII + III. The virion-extracted DNA (Mr5 x 10(6)) of cauliflower mosaic virus (CaMV) has three single-stranded interruptions. The mapping of this DNA using eleven restriction endonucleases (HhaI, SacI, AvaI, PvuII, PstI, XbaI, EcoRI, Bg/II, HincII, HpaII and HindII + III) is reported here. The existence of the three single-stranded breaks complicates the identification and the molecular weight determination of fragments produced by HpaII, HindIII and HindII + III. Indeed the electrophoretic mobility of some fragments in which a single-stranded discontinuity is located is modified, and the fluorescence of ethidium bromide complexed with these fragments is reduced as compared to that observed for the other fragments existing in a molar ratio. These drawbacks were overcome by performing experiments of nick-translation of CaMV DNA with Escherichia coli DNA polymerase I. FRom the data it follows that the CaMV DNA molecule bears bears 1 site for HhaI and SacI, 2 for AvaI and PvuII, 3 for PstI, 4 for XbaI, 5 for EcoRI, 6 for Bg/II and HincII, 11 for HpaII and 15 for HindII + III. The corresponding fragments have all been ordered and precisely located providing a suitable map for further investigations connected with the study of the fine structure and the function of the CaMV genome."}
{"id": "PMID:488096", "title": "Messenger ribonucleoprotein complexes in human KB cells infected with adenovirus type 5 contain tightly bound viral-coded '100K' proteins.", "content": "Late after infection of KB cells with adenovirus 5 an extra protein becomes associated with messenger ribonucleoprotein particles present in the polysomes. This protein has a molecular weight of 100000 and is identical to the virus coded '100K' protein found previously. The extra protein is firmly attached to the messenger ribonucleoprotein complexes. Its binding resists exposure to high salt concentrations as used in puromycin/high-salt dissociation and equilibrium centrifugation in Cs2SO4 gradients. In this respect it resembles the binding of two other proteins of Mr 74000 and 48000 which are commonly found in messenger ribonucleoprotein particles of various eukaryotic cells. The identity between the messenger ribonucleoprotein protein of Mr 100000 and the \"100K' protein present in the soluble part of the cytoplasm was established by sodium dodecylsulphate/polyacrylamide gel electrophoresis, isoelectric focusing and peptide mapping after limited proteolysis with Staphylococcus aureus protease.", "contents": "Messenger ribonucleoprotein complexes in human KB cells infected with adenovirus type 5 contain tightly bound viral-coded '100K' proteins. Late after infection of KB cells with adenovirus 5 an extra protein becomes associated with messenger ribonucleoprotein particles present in the polysomes. This protein has a molecular weight of 100000 and is identical to the virus coded '100K' protein found previously. The extra protein is firmly attached to the messenger ribonucleoprotein complexes. Its binding resists exposure to high salt concentrations as used in puromycin/high-salt dissociation and equilibrium centrifugation in Cs2SO4 gradients. In this respect it resembles the binding of two other proteins of Mr 74000 and 48000 which are commonly found in messenger ribonucleoprotein particles of various eukaryotic cells. The identity between the messenger ribonucleoprotein protein of Mr 100000 and the \"100K' protein present in the soluble part of the cytoplasm was established by sodium dodecylsulphate/polyacrylamide gel electrophoresis, isoelectric focusing and peptide mapping after limited proteolysis with Staphylococcus aureus protease."}
{"id": "PMID:488097", "title": "Purification and properties of alanine dehydrogenase from Bacillus sphaericus.", "content": "1. The bacterial distribution of alanine dehydrogenase (L-alanine:NAD+ oxidoreductase, deaminating, EC 1.4.1.1) was investigated, and high activity was found in Bacillus species. The enzyme has been purified to homogeneity and crystallized from B. sphaericus (IFO 3525), in which the highest activity occurs. 2. The enzyme has a molecular weight of about 230 000, and is composed of six identical subunits (Mr 38 000). 3. The enzyme acts almost specifically on L-alanine, but shows low amino-acceptor specificity; pyruvate and 2-oxobutyrate are the most preferable substrates, and 2-oxovalerate is also animated. The enzyme requires NAD+ as a cofactor, which cannot be replaced by NADP+. 4. The enzyme is stable over a wide pH range (pH 6.0--10.0), and shows maximum reactivity at approximately pH 10.5 and 9.0 for the deamination and amination reactions, respectively. 5. Alanine dehydrogenase is inhibited significantly by HgCl2, p-chloromercuribenzoate and other metals, but none of purine and pyrimidine bases, nucleosides, nucleotides, flavine compounds and pyridoxal 5'-phosphate influence the activity. 6. The reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds first to the enzyme followed by ammonia and pyruvate, and the products are released in the order of L-ALANINE AND NAD+. The Michaelis constants are as follows: NADH (10 microM), ammonia (28.2 mM), pyruvate (1.7 mM), L-alanine (18.9 mM) and NAD+ (0.23 mM). 7. The pro-R hydrogen at C-4 of the reduced nicotinamide ring of NADH is exclusively transferred to pyruvate; the enzyme is A-stereospecific.", "contents": "Purification and properties of alanine dehydrogenase from Bacillus sphaericus. 1. The bacterial distribution of alanine dehydrogenase (L-alanine:NAD+ oxidoreductase, deaminating, EC 1.4.1.1) was investigated, and high activity was found in Bacillus species. The enzyme has been purified to homogeneity and crystallized from B. sphaericus (IFO 3525), in which the highest activity occurs. 2. The enzyme has a molecular weight of about 230 000, and is composed of six identical subunits (Mr 38 000). 3. The enzyme acts almost specifically on L-alanine, but shows low amino-acceptor specificity; pyruvate and 2-oxobutyrate are the most preferable substrates, and 2-oxovalerate is also animated. The enzyme requires NAD+ as a cofactor, which cannot be replaced by NADP+. 4. The enzyme is stable over a wide pH range (pH 6.0--10.0), and shows maximum reactivity at approximately pH 10.5 and 9.0 for the deamination and amination reactions, respectively. 5. Alanine dehydrogenase is inhibited significantly by HgCl2, p-chloromercuribenzoate and other metals, but none of purine and pyrimidine bases, nucleosides, nucleotides, flavine compounds and pyridoxal 5'-phosphate influence the activity. 6. The reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds first to the enzyme followed by ammonia and pyruvate, and the products are released in the order of L-ALANINE AND NAD+. The Michaelis constants are as follows: NADH (10 microM), ammonia (28.2 mM), pyruvate (1.7 mM), L-alanine (18.9 mM) and NAD+ (0.23 mM). 7. The pro-R hydrogen at C-4 of the reduced nicotinamide ring of NADH is exclusively transferred to pyruvate; the enzyme is A-stereospecific."}
{"id": "PMID:488098", "title": "Kinetic and binding properties of the oxoglutarate translocator of rat-heart mitochondria.", "content": "The kinetic study of the oxoglutarateout/malatein exchange through the inner mitochondrial membrane of rat-heart mitochondria has been compelted and extended to higher external-oxoglutarate and to lower internal-malate concentrations. It has been found that the external oxoglutarate inhibits the exchange at high concentration. This excess-substrate inhibition is preceded by four jumps. The kinetic-saturation curve by the internal malate presents an apparent positive cooperativity that may be interpreted in different ways. The independence of the effects of the two substrates on the initial rate has been observed again and supports the conclusions reached in previous work. A method for the determination of oxoglutarate binding to the external face of the inner membrane is described. The binding curve shows four intermediary plateau regions that reflect significant apparent K-effects, alternatively negative and positive. For external-oxoglutarate concentrations below the region of excess-substrate inhibition, the binding-saturation curve and the kinetic-saturation curve are similar, demonstrating that K-effects are predominant. A particularly wide intermediary plateau that seems to correspond to half saturation of the active sites is common to both saturation curves. A clear lack of proportionality between the two curves at low oxoglutarate concentrations seems to indicate that more than one catalytic-rate constant is implied in the exchange kinetics. Two models of the oxoglutarate carrier are presented. Both lead to a minimum degree of 10:10 for the equation of the binding of oxoglutarate to the catalytic sites. In the first model this corresponds to ten subunits associated into a single oligomer while in the second model this results from a mixture of monomeric, dimeric, trimeric and tetrameric associations.", "contents": "Kinetic and binding properties of the oxoglutarate translocator of rat-heart mitochondria. The kinetic study of the oxoglutarateout/malatein exchange through the inner mitochondrial membrane of rat-heart mitochondria has been compelted and extended to higher external-oxoglutarate and to lower internal-malate concentrations. It has been found that the external oxoglutarate inhibits the exchange at high concentration. This excess-substrate inhibition is preceded by four jumps. The kinetic-saturation curve by the internal malate presents an apparent positive cooperativity that may be interpreted in different ways. The independence of the effects of the two substrates on the initial rate has been observed again and supports the conclusions reached in previous work. A method for the determination of oxoglutarate binding to the external face of the inner membrane is described. The binding curve shows four intermediary plateau regions that reflect significant apparent K-effects, alternatively negative and positive. For external-oxoglutarate concentrations below the region of excess-substrate inhibition, the binding-saturation curve and the kinetic-saturation curve are similar, demonstrating that K-effects are predominant. A particularly wide intermediary plateau that seems to correspond to half saturation of the active sites is common to both saturation curves. A clear lack of proportionality between the two curves at low oxoglutarate concentrations seems to indicate that more than one catalytic-rate constant is implied in the exchange kinetics. Two models of the oxoglutarate carrier are presented. Both lead to a minimum degree of 10:10 for the equation of the binding of oxoglutarate to the catalytic sites. In the first model this corresponds to ten subunits associated into a single oligomer while in the second model this results from a mixture of monomeric, dimeric, trimeric and tetrameric associations."}
{"id": "PMID:488099", "title": "Cysteinyl-tRNA synthetase from Bacillus stearothermophilus. A structural and functional monomer.", "content": "A procedure is described for the purification of cysteinyl-tRNA synthetase as a side product of a multi-enzyme isolation from Bacillus stearothermophilus. The native and denatured enzyme are both shown to have a molecular weight of 54000 by gel filtration and sodium dodecyl sulphate/polyacrylamide gel electrophoresis respectively. Fingerprinting and peptide counting indicate that the polypeptide chain has a nonrepeating primary structure. The enzyme has only one binding site for each of its substrates (cysteine, ATP and tRNACys) as judged by equilibrium dialysis, active-site titration and fluorescence quenching. No evidence for the dimerisation of the enzyme in the presence of these substrates could be found. We conclude that cysteinyl-tRNA synthetase, which is the smallest aminoacyl-tRNA synthetase yet described, is both structurally and functionally monomeric.", "contents": "Cysteinyl-tRNA synthetase from Bacillus stearothermophilus. A structural and functional monomer. A procedure is described for the purification of cysteinyl-tRNA synthetase as a side product of a multi-enzyme isolation from Bacillus stearothermophilus. The native and denatured enzyme are both shown to have a molecular weight of 54000 by gel filtration and sodium dodecyl sulphate/polyacrylamide gel electrophoresis respectively. Fingerprinting and peptide counting indicate that the polypeptide chain has a nonrepeating primary structure. The enzyme has only one binding site for each of its substrates (cysteine, ATP and tRNACys) as judged by equilibrium dialysis, active-site titration and fluorescence quenching. No evidence for the dimerisation of the enzyme in the presence of these substrates could be found. We conclude that cysteinyl-tRNA synthetase, which is the smallest aminoacyl-tRNA synthetase yet described, is both structurally and functionally monomeric."}
{"id": "PMID:488101", "title": "Turnover rates of the molecular species of alkenyl ether phospholipids of Ehrlich ascites tumor cells.", "content": "[1-14C]Glycerol was injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells and the lipids were extracted from the cells after selected periods. 1-O-Alkenyl-2-acyl-3-acetylglycerols were prepared from the ethanolamine phosphoglycerides and fractionated by AgNO3-impregnated thin-layer chromatography into five molecular species. Bands 1--5 obtained in this way can be designated as saturated, monoene, diene, tetraene and hexaene species, respectively. Band 5, composed mainly of hexaene species was predominant among those derived from alkenyl ether phospholipids. The specific radioactivities of the fractionated species were determined. Hexaene molecular species turned over most rapidly and disaturated and tetraene species turned over at a high rate, while monoene and diene molecular species turned over very slowly.", "contents": "Turnover rates of the molecular species of alkenyl ether phospholipids of Ehrlich ascites tumor cells. [1-14C]Glycerol was injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells and the lipids were extracted from the cells after selected periods. 1-O-Alkenyl-2-acyl-3-acetylglycerols were prepared from the ethanolamine phosphoglycerides and fractionated by AgNO3-impregnated thin-layer chromatography into five molecular species. Bands 1--5 obtained in this way can be designated as saturated, monoene, diene, tetraene and hexaene species, respectively. Band 5, composed mainly of hexaene species was predominant among those derived from alkenyl ether phospholipids. The specific radioactivities of the fractionated species were determined. Hexaene molecular species turned over most rapidly and disaturated and tetraene species turned over at a high rate, while monoene and diene molecular species turned over very slowly."}
{"id": "PMID:488102", "title": "Structure of Helix pomatia oxy-beta-hemocyanin and deoxy-beta-hemocyanin tubular polymers.", "content": "Mild trypsinolysis of Helix pomatia beta-hemocyanin leads to the formation of tubular polymers after removal of the collar part [van Breemen, J.F.L., Wichertjes, T., Muller, M.F.J., van Driel, R., and van Bruggen, E.F.J. (1975) Eur. J. Biochem. 60, 129--135]. Three-dimensional image reconstruction from electron micrographs of negatively stained tubular polymers showed: (a) alternating deep and shallow grooves in between the 10 helical chains, (b) the presence and position of two domains within each morphological wall-unit of the Mellema and Klug model [Mellema, J. E. and Klug, A. (1972) Nature (Lond.) 239, 146--150]. Optical diffraction of oxy and deoxygenated tubular polymers indicate a significant decrease in diameter with a concomitant increase in length upon deoxygenation.", "contents": "Structure of Helix pomatia oxy-beta-hemocyanin and deoxy-beta-hemocyanin tubular polymers. Mild trypsinolysis of Helix pomatia beta-hemocyanin leads to the formation of tubular polymers after removal of the collar part [van Breemen, J.F.L., Wichertjes, T., Muller, M.F.J., van Driel, R., and van Bruggen, E.F.J. (1975) Eur. J. Biochem. 60, 129--135]. Three-dimensional image reconstruction from electron micrographs of negatively stained tubular polymers showed: (a) alternating deep and shallow grooves in between the 10 helical chains, (b) the presence and position of two domains within each morphological wall-unit of the Mellema and Klug model [Mellema, J. E. and Klug, A. (1972) Nature (Lond.) 239, 146--150]. Optical diffraction of oxy and deoxygenated tubular polymers indicate a significant decrease in diameter with a concomitant increase in length upon deoxygenation."}
{"id": "PMID:488103", "title": "Diversity and complexity of uterine mRNA from rats of differing hormonal states.", "content": "The diversity and complexity of the uterine mRNA population has been compared at two different stages of uterine growth and development in the rat. Analysis by cDNA hybridization to homologous mRNA indicates that there are 8000 different sequences expressed in the immature rat uterus responding to just 4 h of oestradiol-17 beta-induced growth while the fully developed uterus expresses 36 000 sequences. As a check on our results, the complexity is re-analysed by hybridization of mercurated mRNA to total unique DNA. Analysis by this method indicates that the hormone-stimulated immature rat uterus contains 12 000 poly(A)-containing mRNA sequences while the fully differentiated adult tissue contains 53 000 diverse sequences.", "contents": "Diversity and complexity of uterine mRNA from rats of differing hormonal states. The diversity and complexity of the uterine mRNA population has been compared at two different stages of uterine growth and development in the rat. Analysis by cDNA hybridization to homologous mRNA indicates that there are 8000 different sequences expressed in the immature rat uterus responding to just 4 h of oestradiol-17 beta-induced growth while the fully developed uterus expresses 36 000 sequences. As a check on our results, the complexity is re-analysed by hybridization of mercurated mRNA to total unique DNA. Analysis by this method indicates that the hormone-stimulated immature rat uterus contains 12 000 poly(A)-containing mRNA sequences while the fully differentiated adult tissue contains 53 000 diverse sequences."}
{"id": "PMID:488104", "title": "Qualitative and quantitative changes in uterine mRNA populations in response to oestradiol treatment of rats.", "content": "The mRNA population of immature rat uteri, responding to different lengths of oestradiol-induced differentiation, have been compared by the technique of DNA hybridization with heterogeneous RNA. Over the first 4 h of hormone induction there are continuing qualitative and quantitative changes in the poly(A)-containing mRNA such that, by 4 h after oestradiol administration, the population bears little resemblance to that of the unstimulated animal. Between 2 h and 4 h after oestradiol administration, the most striking changes appear to be in sequences of intermediate abundance. The possible significance of these findings is discussed.", "contents": "Qualitative and quantitative changes in uterine mRNA populations in response to oestradiol treatment of rats. The mRNA population of immature rat uteri, responding to different lengths of oestradiol-induced differentiation, have been compared by the technique of DNA hybridization with heterogeneous RNA. Over the first 4 h of hormone induction there are continuing qualitative and quantitative changes in the poly(A)-containing mRNA such that, by 4 h after oestradiol administration, the population bears little resemblance to that of the unstimulated animal. Between 2 h and 4 h after oestradiol administration, the most striking changes appear to be in sequences of intermediate abundance. The possible significance of these findings is discussed."}
{"id": "PMID:488105", "title": "A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant.", "content": "A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes: (a) from the ribosomal wash and (b) from the postribosomal supernantant. A comparison is made between these preparations with respect to yield and specific activity. eIF-4A and eIF-4D occur mainly in the postribosomal supernatant; eIF-2, eIF-4C and eIF-5 are more evenly divided over both fractions, whereas eIF-1, eIF-3 and eIF-4B are found almost exclusively in the ribosomal wash. No significant difference in specific activity could be detected when factors from both sources were compared, with a possible exception of eIF-4A and eIF-4D.", "contents": "A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant. A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes: (a) from the ribosomal wash and (b) from the postribosomal supernantant. A comparison is made between these preparations with respect to yield and specific activity. eIF-4A and eIF-4D occur mainly in the postribosomal supernatant; eIF-2, eIF-4C and eIF-5 are more evenly divided over both fractions, whereas eIF-1, eIF-3 and eIF-4B are found almost exclusively in the ribosomal wash. No significant difference in specific activity could be detected when factors from both sources were compared, with a possible exception of eIF-4A and eIF-4D."}
{"id": "PMID:488106", "title": "A centrifugation study of rat-liver mitochondria, lysosomes and peroxisomes during the perinatal period.", "content": "We have investigated the intracellular distribution of several enzymes on homogenates of late foetal, early postnatal and adult rat livers. Homogenates were subjected to differential centrifugations in 0.25 M sucrose and four fractions were isolated which corresponded to the N (nuclear) ML (total mitochondrial) P (microsomal) and S (soluble) fractions of de Duve et al. (1955). In general the age of the animal did not significantly affect the distribution pattern. Reference enzymes of mitochondria, lysosomes and peroxisomes were mainly recovered in the total mitochondrial fraction (ML). Glucose-6-phosphatase and esterase, both located in the endoplasmic reticulum, were chiefly associated with the microsomal fraction P together with galactosyltransferase (a reference enzyme of the Golgi apparatus). 5'-Nucleotidase, (a plasma membrane enzyme) exhibits a bimodal distribution and is mainly recovered in the N and the P fractions. Such results indicate that the membrane composition of the fractions isolated by the fractionation scheme was used, does not appreciably differ for the late foetal, early postnatal and adult rat livers. An analytical fractionation of the mitochondrial (ML) fraction of livers at different stages of development was performed by isopycnic centrifugation in sucrose gradients and in glycogen gradients using sucrose solutions of various concentrations as the solvents. The distribution of mitochondria, lysosomes and peroxisomes were assessed by establishing the distribution of their reference enzymes. Some physical characteristics of the particles were deduced from the manner in which the distributions were influenced by the sucrose concentration of the centrifugation medium. The distribution of liver mitochondrial enzymes one day prenatal differs strikingly from that of enzymes one day postnatal; foetal mitochondria seem characterized by a high osmotic space and a high hydrated matrix density; neonatal mitochondria seem devoid of an osmotic space and the density of their hydrated matrix is markedly lower than that of the foetal mitochondria. As ascertained by the distribution of mitochondrial enzymes in a sucrose 2H2O gradient, the high density of a foetal mitochondria matrix does not mainly originate from a lower amount of hydration water. The behavior of lysosomal enzymes in media with increasing concentrations of sucrose suggests that lysosomes originating from late foetal rat liver are endowed with a very small osmotic space. As for the peroxisomes, our results do not display significant behavior differences in centrifugations that would indicate physicochemical changes of these particles during the perinatal period.", "contents": "A centrifugation study of rat-liver mitochondria, lysosomes and peroxisomes during the perinatal period. We have investigated the intracellular distribution of several enzymes on homogenates of late foetal, early postnatal and adult rat livers. Homogenates were subjected to differential centrifugations in 0.25 M sucrose and four fractions were isolated which corresponded to the N (nuclear) ML (total mitochondrial) P (microsomal) and S (soluble) fractions of de Duve et al. (1955). In general the age of the animal did not significantly affect the distribution pattern. Reference enzymes of mitochondria, lysosomes and peroxisomes were mainly recovered in the total mitochondrial fraction (ML). Glucose-6-phosphatase and esterase, both located in the endoplasmic reticulum, were chiefly associated with the microsomal fraction P together with galactosyltransferase (a reference enzyme of the Golgi apparatus). 5'-Nucleotidase, (a plasma membrane enzyme) exhibits a bimodal distribution and is mainly recovered in the N and the P fractions. Such results indicate that the membrane composition of the fractions isolated by the fractionation scheme was used, does not appreciably differ for the late foetal, early postnatal and adult rat livers. An analytical fractionation of the mitochondrial (ML) fraction of livers at different stages of development was performed by isopycnic centrifugation in sucrose gradients and in glycogen gradients using sucrose solutions of various concentrations as the solvents. The distribution of mitochondria, lysosomes and peroxisomes were assessed by establishing the distribution of their reference enzymes. Some physical characteristics of the particles were deduced from the manner in which the distributions were influenced by the sucrose concentration of the centrifugation medium. The distribution of liver mitochondrial enzymes one day prenatal differs strikingly from that of enzymes one day postnatal; foetal mitochondria seem characterized by a high osmotic space and a high hydrated matrix density; neonatal mitochondria seem devoid of an osmotic space and the density of their hydrated matrix is markedly lower than that of the foetal mitochondria. As ascertained by the distribution of mitochondrial enzymes in a sucrose 2H2O gradient, the high density of a foetal mitochondria matrix does not mainly originate from a lower amount of hydration water. The behavior of lysosomal enzymes in media with increasing concentrations of sucrose suggests that lysosomes originating from late foetal rat liver are endowed with a very small osmotic space. As for the peroxisomes, our results do not display significant behavior differences in centrifugations that would indicate physicochemical changes of these particles during the perinatal period."}
{"id": "PMID:488107", "title": "Stereochemistry of reactions catalysed by mammalian-brain L-glutamate 1-carboxy-lyase and 4-aminobutyrate: 2-oxoglutarate aminotransferase.", "content": "Deamination of 4-aminobutyrate by mammalian or bacterial 4-aminobutyrate aminotransferases involves the abstraction of the pro-S hydrogen on C-4 of 4-aminobutyrate. Decarboxylation of L-glutamate by rat brain glutamate decarboxylase occurs with retention of configuration. Inhibition of this enzyme by (S)-4-aminohex-5-ynoic acid involves the abstraction of the proton at C-4 of the inhibitor. On the basis of this finding, we postulate the existence of an abnormal reaction of glutamate decarboxylase in which the proton at C-4 of (S)-4-aminohex-5-ynoic acid is removed in a manner similar to the one which normally occurs in enzymatic transaminations of L-amino acids. This reaction is presumably facilitated by the acetylenic group adjacent to the eliminated proton.", "contents": "Stereochemistry of reactions catalysed by mammalian-brain L-glutamate 1-carboxy-lyase and 4-aminobutyrate: 2-oxoglutarate aminotransferase. Deamination of 4-aminobutyrate by mammalian or bacterial 4-aminobutyrate aminotransferases involves the abstraction of the pro-S hydrogen on C-4 of 4-aminobutyrate. Decarboxylation of L-glutamate by rat brain glutamate decarboxylase occurs with retention of configuration. Inhibition of this enzyme by (S)-4-aminohex-5-ynoic acid involves the abstraction of the proton at C-4 of the inhibitor. On the basis of this finding, we postulate the existence of an abnormal reaction of glutamate decarboxylase in which the proton at C-4 of (S)-4-aminohex-5-ynoic acid is removed in a manner similar to the one which normally occurs in enzymatic transaminations of L-amino acids. This reaction is presumably facilitated by the acetylenic group adjacent to the eliminated proton."}
{"id": "PMID:488108", "title": "Deuterium isotope effects on papain acylation. Evidence for lack of general base catalysis and for enzyme--leaving-group interaction.", "content": "The experimental data presented in this paper comprise kinetic deuterium isotope effects on acylation of papain with various substrates when conducted in H2O and 2H2O. With alkyl esters of N-acylamino acids there is no or very little isotope effect, whereas with N-acylamino acid amides the ratio kappa H2O/kappa 2H2O is less than 1, i.e. there is an inverse isotope effect. Similarly, alkylation of papain with methyl bromoacetate exhibits no kinetic isotope effect, whereas for the analogous alkylation with bromoacetamide an inverse isotope effect is observed. It is concluded that (a) general base catalysis does not occur in the acylation of papain and (b) kinetic deuterium isotope effects can be affected substantially by interaction between the substrate leaving group and the enzyme, which has not been considered in previous mechanistic investigations.", "contents": "Deuterium isotope effects on papain acylation. Evidence for lack of general base catalysis and for enzyme--leaving-group interaction. The experimental data presented in this paper comprise kinetic deuterium isotope effects on acylation of papain with various substrates when conducted in H2O and 2H2O. With alkyl esters of N-acylamino acids there is no or very little isotope effect, whereas with N-acylamino acid amides the ratio kappa H2O/kappa 2H2O is less than 1, i.e. there is an inverse isotope effect. Similarly, alkylation of papain with methyl bromoacetate exhibits no kinetic isotope effect, whereas for the analogous alkylation with bromoacetamide an inverse isotope effect is observed. It is concluded that (a) general base catalysis does not occur in the acylation of papain and (b) kinetic deuterium isotope effects can be affected substantially by interaction between the substrate leaving group and the enzyme, which has not been considered in previous mechanistic investigations."}
{"id": "PMID:488110", "title": "Site-specific substituted cobalt(II) horse liver alcohol dehydrogenases. Preparation and characterization in solution, crystalline and immobilized state.", "content": "The specific substitution, using highly selective techniques, of catalytic and/or noncatalytic zinc ions by cobaltous ions in horse liver alcohol dehydrogenase (EC 1.1.1.1) has been studied with dissolved, crystalline and agarose-immobilised enzyme, in order to examine the effect of protein structure on the specificity of the metal exchange. The different binding sites can be clearly distinguished by the absorption spectra of their cobalt derivatives. In solution an anaerobic column chromatographic method made it possible to exchange half of the zinc in the enzyme by cobalt ions in a much shorter time than previous procedures. By raising the temperature in the exchange step, even the slowly exchanging zinc ions were substituted by cobalt, yielding products similar to cobalt alcohol dehydrogenases described earlier. Treatment of crystal suspensions of the enzyme with chelating agents (preferentially dipicolinic acid) gave an inactive protein with two zinc ions remaining bound. The enzyme could be reactivated by treatment of the crystalline protein with 5 mM zinc or cobaltous ions or by dialysis of dissolved inactive protein against 20 microM zinc or 1 mM cobaltous ions. Higher metal concentrations led to denaturation but the inactive protein could be crystallized from solution and then reactivated completely at higher metal concentrations. The preparation and absorption spectrum show that cobalt is bound specifically at the catalytic sites. Since metal substitution at these sites critically depends on the maintenance of the correct tertiary and quaternary structure, these must be preserved in the crystal lattice and partially altered in solution when the catalytic zinc ions are removed (or when excess of metal ions is applied), thus demonstrating the structure-stabilizing role of the catalytic metal ions. The enzyme immobilised on agarose, with unchanged content of active sites [Schneider-Bernl\u00f6hr et al. (1978) Eur. J. Biochem. 41, 475--484], was treated like the crystal suspensions. Although half of the zinc was removed, some activity remained. After reactivation with cobaltous ions, a loss of about 30% active sites was measured. Thus the apparently homogenous bound enzyme was rather heterogeneous in the properties of its catalytic metal binding sites. These results are taken as further proof for the dependence of the metal substitution on the proper tertiary and quaternary structure which is strained by multiple interactions in the covalently immobilised enzyme.", "contents": "Site-specific substituted cobalt(II) horse liver alcohol dehydrogenases. Preparation and characterization in solution, crystalline and immobilized state. The specific substitution, using highly selective techniques, of catalytic and/or noncatalytic zinc ions by cobaltous ions in horse liver alcohol dehydrogenase (EC 1.1.1.1) has been studied with dissolved, crystalline and agarose-immobilised enzyme, in order to examine the effect of protein structure on the specificity of the metal exchange. The different binding sites can be clearly distinguished by the absorption spectra of their cobalt derivatives. In solution an anaerobic column chromatographic method made it possible to exchange half of the zinc in the enzyme by cobalt ions in a much shorter time than previous procedures. By raising the temperature in the exchange step, even the slowly exchanging zinc ions were substituted by cobalt, yielding products similar to cobalt alcohol dehydrogenases described earlier. Treatment of crystal suspensions of the enzyme with chelating agents (preferentially dipicolinic acid) gave an inactive protein with two zinc ions remaining bound. The enzyme could be reactivated by treatment of the crystalline protein with 5 mM zinc or cobaltous ions or by dialysis of dissolved inactive protein against 20 microM zinc or 1 mM cobaltous ions. Higher metal concentrations led to denaturation but the inactive protein could be crystallized from solution and then reactivated completely at higher metal concentrations. The preparation and absorption spectrum show that cobalt is bound specifically at the catalytic sites. Since metal substitution at these sites critically depends on the maintenance of the correct tertiary and quaternary structure, these must be preserved in the crystal lattice and partially altered in solution when the catalytic zinc ions are removed (or when excess of metal ions is applied), thus demonstrating the structure-stabilizing role of the catalytic metal ions. The enzyme immobilised on agarose, with unchanged content of active sites [Schneider-Bernl\u00f6hr et al. (1978) Eur. J. Biochem. 41, 475--484], was treated like the crystal suspensions. Although half of the zinc was removed, some activity remained. After reactivation with cobaltous ions, a loss of about 30% active sites was measured. Thus the apparently homogenous bound enzyme was rather heterogeneous in the properties of its catalytic metal binding sites. These results are taken as further proof for the dependence of the metal substitution on the proper tertiary and quaternary structure which is strained by multiple interactions in the covalently immobilised enzyme."}
{"id": "PMID:488111", "title": "Purification and characterization of a protein factor that reverses the inhibition of protein synthesis by the heme-regulated translational inhibitor in rabbit reticulocyte lysates.", "content": "We have purified and partially characterized a supernatant factor which reverses the effect of the heme-regulated translational inhibitor on protein synthesis in rabbit reticulocyte lysates. The anti-inhibitor restores protein synthesis activity in heme deficient lysates (and in lysates to which the inhibitor has been added) to the level observed in the presence of heme. The factor has no effect on the phosphorylation of eIF-2 by the inhibitor nor on any reaction carried out with purified initiation factors. The anti-inhibitor probably consists of three subunits with molecular weights of 81000, 60000 and 41000. The factor is isolated from the postribosomal supernatant of rabbit reticulocytes both free and complexed to eIF-2. A possible mechanism of action is discussed.", "contents": "Purification and characterization of a protein factor that reverses the inhibition of protein synthesis by the heme-regulated translational inhibitor in rabbit reticulocyte lysates. We have purified and partially characterized a supernatant factor which reverses the effect of the heme-regulated translational inhibitor on protein synthesis in rabbit reticulocyte lysates. The anti-inhibitor restores protein synthesis activity in heme deficient lysates (and in lysates to which the inhibitor has been added) to the level observed in the presence of heme. The factor has no effect on the phosphorylation of eIF-2 by the inhibitor nor on any reaction carried out with purified initiation factors. The anti-inhibitor probably consists of three subunits with molecular weights of 81000, 60000 and 41000. The factor is isolated from the postribosomal supernatant of rabbit reticulocytes both free and complexed to eIF-2. A possible mechanism of action is discussed."}
{"id": "PMID:488113", "title": "Sodium and calcium binding to Panulirus interruptus hemocyanin as studied by 23Na nuclear magnetic resonance.", "content": "Addition of Panulirus hemocyanin to NaCl solutions produces marked changes in the 23Na relaxation parameters; they show that sodium ions interact with binding sites on the protein and exchange rapidly with the bulk. The observed non-lorentzian lineshapes and the non-exponential decay of the transverse magnetization indicate that non-extreme narrowing conditions apply and give information on the dynamics of the interaction. Panulirus hemocyanin has at least two classes of Na+ binding sites; the binding constant of the more strongly bound sodium ions is in the order of 1 X 10(2) M-1. Competition between Na+ and Ca2+ for protein binding sites is demonstrated by the effect of Ca2+ on the 23Na relaxation parameters. However, only the more strongly bound Na+ are displaced by Ca2+. The number of Ca2+ needed to displace these sodium ions is 3--5 per oxygen binding site. The 23Na relaxation parameters are influenced also by the state of oxygenation of the protein, indicating a linkage between Na+ and oxygen binding. The simplest interpretation of the data is that sodium ions bind more strongly to oxyhemocyanin in agreement with oxygen equilibrium experiments.", "contents": "Sodium and calcium binding to Panulirus interruptus hemocyanin as studied by 23Na nuclear magnetic resonance. Addition of Panulirus hemocyanin to NaCl solutions produces marked changes in the 23Na relaxation parameters; they show that sodium ions interact with binding sites on the protein and exchange rapidly with the bulk. The observed non-lorentzian lineshapes and the non-exponential decay of the transverse magnetization indicate that non-extreme narrowing conditions apply and give information on the dynamics of the interaction. Panulirus hemocyanin has at least two classes of Na+ binding sites; the binding constant of the more strongly bound sodium ions is in the order of 1 X 10(2) M-1. Competition between Na+ and Ca2+ for protein binding sites is demonstrated by the effect of Ca2+ on the 23Na relaxation parameters. However, only the more strongly bound Na+ are displaced by Ca2+. The number of Ca2+ needed to displace these sodium ions is 3--5 per oxygen binding site. The 23Na relaxation parameters are influenced also by the state of oxygenation of the protein, indicating a linkage between Na+ and oxygen binding. The simplest interpretation of the data is that sodium ions bind more strongly to oxyhemocyanin in agreement with oxygen equilibrium experiments."}
{"id": "PMID:488114", "title": "Chloroplast phosphoproteins. Phosphorylation of polypeptides of the light-harvesting chlorophyll protein complex.", "content": "When isolated, intact chloroplasts of pea (Pisum sativum) are incubated in the light with [32P]-orthophosphate, isotope is incorporated into several polypeptides. Among the most conspicuous phosphoproteins are two which form a very closely spaced doublet on dodecyl sulphate/polyacrylamide gels and co-electrophorese with the major polypeptide component of the light-harvesting chlorophyll a/b binding complex. Like the light-harvesting polypeptide, the phosphoprotein doublet is bound to thylakoids, sediments with the heavy particles released from thylakoids after digitonin treatment, is soluble in chloroform/methanol and has an apparent molecular weight of about 26 000. The doublet also appears in the highly purified light-harvesting chlorophyll a/b binding complex isolated from thylakoids by hydrosylapatite chromatography. I conclude that two polypeptide components of the complex are phosphorylated. One of these components may be the major light-harvesting chlorophyll a/b binding protein.", "contents": "Chloroplast phosphoproteins. Phosphorylation of polypeptides of the light-harvesting chlorophyll protein complex. When isolated, intact chloroplasts of pea (Pisum sativum) are incubated in the light with [32P]-orthophosphate, isotope is incorporated into several polypeptides. Among the most conspicuous phosphoproteins are two which form a very closely spaced doublet on dodecyl sulphate/polyacrylamide gels and co-electrophorese with the major polypeptide component of the light-harvesting chlorophyll a/b binding complex. Like the light-harvesting polypeptide, the phosphoprotein doublet is bound to thylakoids, sediments with the heavy particles released from thylakoids after digitonin treatment, is soluble in chloroform/methanol and has an apparent molecular weight of about 26 000. The doublet also appears in the highly purified light-harvesting chlorophyll a/b binding complex isolated from thylakoids by hydrosylapatite chromatography. I conclude that two polypeptide components of the complex are phosphorylated. One of these components may be the major light-harvesting chlorophyll a/b binding protein."}
{"id": "PMID:488115", "title": "THE DNA components of the chicken genome.", "content": "The organization of the chicken genome was investigated by centrifuging chicken DNA (Mr = 57 X 10(6) in preparative Cs2SO4/Ag+ and Cs2SO4/BAMD density gradients [BAMD = 3.6-bis(acetato-mercurimethyl)dioxane]. An analysis by CsCl density gradient of the DNA fractions obtained from the preparative experiments revealed that 88% of the genome is made up of four DNA components, characterized by buoyant densities of 1.699, 1.702(5), 1.704(5) and 1.708 g/cm3 and representing 39%, 25%, 15%, and 9%, respectively, of the total DNA. The remaining 12% of the genome is formed by seven minor and/or satellite components. The distribution of the ovalbumin gene in a Cs2CO4/BAMD density gradient, as tested with a cloned cDNA probe, coincides with the distribution of the 1.702(5)-g/cm3 component. This shows that the DNA regions flanking the ovalbumin gene are homogeneous in base composition over along distances and that the gene is located on a DNA segment belonging to the 1.702(5)-g/cm3 component.", "contents": "THE DNA components of the chicken genome. The organization of the chicken genome was investigated by centrifuging chicken DNA (Mr = 57 X 10(6) in preparative Cs2SO4/Ag+ and Cs2SO4/BAMD density gradients [BAMD = 3.6-bis(acetato-mercurimethyl)dioxane]. An analysis by CsCl density gradient of the DNA fractions obtained from the preparative experiments revealed that 88% of the genome is made up of four DNA components, characterized by buoyant densities of 1.699, 1.702(5), 1.704(5) and 1.708 g/cm3 and representing 39%, 25%, 15%, and 9%, respectively, of the total DNA. The remaining 12% of the genome is formed by seven minor and/or satellite components. The distribution of the ovalbumin gene in a Cs2CO4/BAMD density gradient, as tested with a cloned cDNA probe, coincides with the distribution of the 1.702(5)-g/cm3 component. This shows that the DNA regions flanking the ovalbumin gene are homogeneous in base composition over along distances and that the gene is located on a DNA segment belonging to the 1.702(5)-g/cm3 component."}
{"id": "PMID:488116", "title": "Isolation and purification of a neurodepressing hormone from the eyestalk of Procambarus bouvieri (Ortmann).", "content": "1. A neurodepressing hormone has been isolated and purified to homogeneity from aqueous extracts of 2000 eyestalks of the Mexican crayfish Procambarus bouvieri (Ortmann). 2. Purification was achieved by gel filtration on Sephadex G-25 and G-15, and preparative paper electrophoresis at four pH valueimately 1200 molecular weight and composed of neutral amino acids. 5. No N-terminal group could be found. From its electrophoretic behavior it is concluded that the C-terminal group is also blocked.", "contents": "Isolation and purification of a neurodepressing hormone from the eyestalk of Procambarus bouvieri (Ortmann). 1. A neurodepressing hormone has been isolated and purified to homogeneity from aqueous extracts of 2000 eyestalks of the Mexican crayfish Procambarus bouvieri (Ortmann). 2. Purification was achieved by gel filtration on Sephadex G-25 and G-15, and preparative paper electrophoresis at four pH valueimately 1200 molecular weight and composed of neutral amino acids. 5. No N-terminal group could be found. From its electrophoretic behavior it is concluded that the C-terminal group is also blocked."}
{"id": "PMID:488117", "title": "Enrichment and characterization of the DNA coding for vitellogenin in Xenopus laevis.", "content": "Purified vitellogenin mRNA of Xenopus laevis was incubated with mechanically sheared DNA in high concentrations of formamide and the resulting R-loops (i.e. RNA . DNA hybrid fragments) separated from the bulk DNA by caesium chloride buoyant density centrifugation. Hybridization with 125I-labeled vitellogenin mRNA revealed a 15--30-fold enrichment of the DNA coding for vitellogenin. Restriction analysis of the R-loop-enriched DNA demonstrated that all known endonuclease HindIII fragments coding for vitellogenin of unfractionated Xenopus DNA were also present in the enriched material, including the specific fragments for the oligo(A)-containing segment of the RNA. Comparison of these restriction data with the structure found in cloned vitellogenin cDNA, indicates the presence of at least one intervening sequence in the genomic DNA coding for vitellogenin.", "contents": "Enrichment and characterization of the DNA coding for vitellogenin in Xenopus laevis. Purified vitellogenin mRNA of Xenopus laevis was incubated with mechanically sheared DNA in high concentrations of formamide and the resulting R-loops (i.e. RNA . DNA hybrid fragments) separated from the bulk DNA by caesium chloride buoyant density centrifugation. Hybridization with 125I-labeled vitellogenin mRNA revealed a 15--30-fold enrichment of the DNA coding for vitellogenin. Restriction analysis of the R-loop-enriched DNA demonstrated that all known endonuclease HindIII fragments coding for vitellogenin of unfractionated Xenopus DNA were also present in the enriched material, including the specific fragments for the oligo(A)-containing segment of the RNA. Comparison of these restriction data with the structure found in cloned vitellogenin cDNA, indicates the presence of at least one intervening sequence in the genomic DNA coding for vitellogenin."}
{"id": "PMID:488118", "title": "Amino acid sequence of the aminoterminal segment of dermatosparactic calf-skin procollagen type I.", "content": "The N-terminal procollagen peptide of the pN alpha 1(I) chain from dermatosparactic calf skin contains 139 amino acid residues. For the determination of the amino acid sequence the procollagen peptide was treated with pyroglutamate aminopeptidase, protease from Staphylococcus aureus V8 and  trypsin. The fragments obtained were separated by molecular sieve and ion-exchange chromatography and submitted to automated Edman degradation. The procollagen peptide consists of three segments, an N-terminal globular domain which contains all the cysteine residues and most of the hydrophobic residues present in the entire peptide, a triple helical part with a relatively high content of proline and hydroxyproline, and a short nonhelical region which forms the connection to the nonhelical region of the alpha 1(I) chain and which contains the proline-glutamine bond specifically split by the N-terminal procollagen peptidase during conversion of procollagen to collagen.", "contents": "Amino acid sequence of the aminoterminal segment of dermatosparactic calf-skin procollagen type I. The N-terminal procollagen peptide of the pN alpha 1(I) chain from dermatosparactic calf skin contains 139 amino acid residues. For the determination of the amino acid sequence the procollagen peptide was treated with pyroglutamate aminopeptidase, protease from Staphylococcus aureus V8 and  trypsin. The fragments obtained were separated by molecular sieve and ion-exchange chromatography and submitted to automated Edman degradation. The procollagen peptide consists of three segments, an N-terminal globular domain which contains all the cysteine residues and most of the hydrophobic residues present in the entire peptide, a triple helical part with a relatively high content of proline and hydroxyproline, and a short nonhelical region which forms the connection to the nonhelical region of the alpha 1(I) chain and which contains the proline-glutamine bond specifically split by the N-terminal procollagen peptidase during conversion of procollagen to collagen."}
{"id": "PMID:488119", "title": "A rare genetically determined variant of psuedocholinesterase in two German families with high plasma enzyme activity.", "content": "Activity of pseudocholinesterase (acylcholine-acyl-hydrolase) elevated up to four times has been detected in sera of members of two German families. The catalytic concentrations of the pseudocholinesterase of the afflicted members of both families (male and female) varied between 4800 U/l and 10 200 U/o (acetylthiocholine iodide substrate). The pseudocholinesterase of the propositi exhibits isoenzyme separation patterns in polyacrylamide electrophoresis as well as in electrofocussing which are different from those of pseudocholinesterase from normal persons. No differences could be seen as regards the Km of substrates or the inhibition by dibucaine, fluoride or succinyldiocholine.", "contents": "A rare genetically determined variant of psuedocholinesterase in two German families with high plasma enzyme activity. Activity of pseudocholinesterase (acylcholine-acyl-hydrolase) elevated up to four times has been detected in sera of members of two German families. The catalytic concentrations of the pseudocholinesterase of the afflicted members of both families (male and female) varied between 4800 U/l and 10 200 U/o (acetylthiocholine iodide substrate). The pseudocholinesterase of the propositi exhibits isoenzyme separation patterns in polyacrylamide electrophoresis as well as in electrofocussing which are different from those of pseudocholinesterase from normal persons. No differences could be seen as regards the Km of substrates or the inhibition by dibucaine, fluoride or succinyldiocholine."}
{"id": "PMID:488120", "title": "Characterization of human platelet proteins solubilized with Triton X-100 and examined by crossed immunoelectrophoresis. Reference patterns of extracts from whole platelets and isolated membranes.", "content": "Whole human platelets and platelet membranes have been solubilized in 1% Triton X-100, and the solubilized proteins examined by crossed immunoelectrophoresis using rabbit antibodies raised against either whole platelets or isolated membranes. 90% of the platelet proteins were solubilized by this extraction. About twenty immunoprecipitates were observed using the extracts obtained from whole platelets, whereas normally eight immunoprecipitates were seen with extracts from isolated membranes. Albumin, factor VIII and fibrinogen were identified with monospecific antibodies. Correlation of the patterns obtained for platelets or membranes was obtained by addition experiments, by crossed-line immunoelectrophoresis and by crossed immunoelectrophoresis of a mixture of extracts from unlabeled whole platelets and membranes isolated from platelets labeled by lactoperoxidase-catalyzed 125I iodination. Four sialoglycoproteins were identified by their reduced electrophoretic migration after neuraminidase treatment, and six proteins interacted with various lectins, indicating them to be glycosylated. Seven amphiphilic proteins were identified by charge-shift crossed immunoelectrophoresis, and nine by crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose. The topographical arrangement of the membrane proteins was examined with lactoperoxidase-catalyzed 125I-labeled platelets as antigens, and by antibodies absorbed with a suspension of whole platelets. Four and six radioactively labeled precipitates could be identified using the platelet and membrane extracts, respectively, indicating them to be exposed at the outer platelet surface. This was confirmed by the use of antibodies absorbed with intact platelets.", "contents": "Characterization of human platelet proteins solubilized with Triton X-100 and examined by crossed immunoelectrophoresis. Reference patterns of extracts from whole platelets and isolated membranes. Whole human platelets and platelet membranes have been solubilized in 1% Triton X-100, and the solubilized proteins examined by crossed immunoelectrophoresis using rabbit antibodies raised against either whole platelets or isolated membranes. 90% of the platelet proteins were solubilized by this extraction. About twenty immunoprecipitates were observed using the extracts obtained from whole platelets, whereas normally eight immunoprecipitates were seen with extracts from isolated membranes. Albumin, factor VIII and fibrinogen were identified with monospecific antibodies. Correlation of the patterns obtained for platelets or membranes was obtained by addition experiments, by crossed-line immunoelectrophoresis and by crossed immunoelectrophoresis of a mixture of extracts from unlabeled whole platelets and membranes isolated from platelets labeled by lactoperoxidase-catalyzed 125I iodination. Four sialoglycoproteins were identified by their reduced electrophoretic migration after neuraminidase treatment, and six proteins interacted with various lectins, indicating them to be glycosylated. Seven amphiphilic proteins were identified by charge-shift crossed immunoelectrophoresis, and nine by crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose. The topographical arrangement of the membrane proteins was examined with lactoperoxidase-catalyzed 125I-labeled platelets as antigens, and by antibodies absorbed with a suspension of whole platelets. Four and six radioactively labeled precipitates could be identified using the platelet and membrane extracts, respectively, indicating them to be exposed at the outer platelet surface. This was confirmed by the use of antibodies absorbed with intact platelets."}
{"id": "PMID:488121", "title": "Growth patterns of rat hepatocytes during postnatal development.", "content": "During postnatal growth in the liver of the rat, a characteristic shift towards binuclear cells and cells of higher ploidy class occurs. When the protein content of individual isolated hepatocytes of different ploidy classes is analysed cytophotometrically using the specific protein stain Naphthol Yellow S, it appears that the growth in mass in the period 30-99 days is due mainly to increase of protein content of binuclear diploid (BD) and mononuclear tetraploid (MT) cells. The mononuclear diploid (MD) cells play a quickly diminishing role in the parenchymal population after the initial growth phase and cells of highest ploidy degree remain unimportant quantitatively. The quickly growing BD and MT cells only reach a Naphthol Yellow S protein value twice that of MD cells after a certain period of growth, whereas changes in protein content are slight or absent from 99 days onwards in all cell types investigated.", "contents": "Growth patterns of rat hepatocytes during postnatal development. During postnatal growth in the liver of the rat, a characteristic shift towards binuclear cells and cells of higher ploidy class occurs. When the protein content of individual isolated hepatocytes of different ploidy classes is analysed cytophotometrically using the specific protein stain Naphthol Yellow S, it appears that the growth in mass in the period 30-99 days is due mainly to increase of protein content of binuclear diploid (BD) and mononuclear tetraploid (MT) cells. The mononuclear diploid (MD) cells play a quickly diminishing role in the parenchymal population after the initial growth phase and cells of highest ploidy degree remain unimportant quantitatively. The quickly growing BD and MT cells only reach a Naphthol Yellow S protein value twice that of MD cells after a certain period of growth, whereas changes in protein content are slight or absent from 99 days onwards in all cell types investigated."}
{"id": "PMID:488122", "title": "[The ultrastructure of epithelial and myoepithelial oncocytes in the human parotid gland (author's transl)].", "content": "Oncocytes are well known to occur in the tissue of many organs for over 30 years. Their origin and function are less well known. We observed different types of oncocytes in a human parotid gland with the electron microscope. Besides a hyperplasia of mitochondria they show more or less advanced signs of membrane degeneration of these organelles. On the basis of our findings we discuss oncocyte formation as a special form of cellular aging in an otherwise healthy gland.", "contents": "[The ultrastructure of epithelial and myoepithelial oncocytes in the human parotid gland (author's transl)]. Oncocytes are well known to occur in the tissue of many organs for over 30 years. Their origin and function are less well known. We observed different types of oncocytes in a human parotid gland with the electron microscope. Besides a hyperplasia of mitochondria they show more or less advanced signs of membrane degeneration of these organelles. On the basis of our findings we discuss oncocyte formation as a special form of cellular aging in an otherwise healthy gland."}
{"id": "PMID:488123", "title": "Stereo-electron microscopy and energy-dispersive x-ray analysis of avian reticulocytes.", "content": "Utilizing the techniques of stereo-electron microscopy (stereo-EM) and energy-dispersive x-ray analysis (EDX), we have studied aspects of the ultrastructure of avian reticulocytes. Stereo-EM of thin sections (0.10 to 0.25 mum thick) stained with uranyl and lead revealed the three-dimensional arrangement of 25 nm chromatin fibers on the tangential surfaces of nuclei. Use of the Bernhard staining procedure in combination with stereo-EM permitted a three-dimensional view of the interchromatin spaces and channels leading to the nuclear pores, and of cytoplasmic polyribosomes. With either staining technique we frequently encountered in the cytoplasm clusters and paracrystalline arrays of electron-dense granules with granule diameters approximately 1/2 that of monomer ribosomes. These granules were highly electron-dense in unstained specimens and have been identified as intracellular ferritin on the basis of the similarity of their ultrastructural morphology to that of horse spleen ferritin and their high content of iron as determined by EDX. The possibility that these granules represent toxic products of phenylhydrazine treatment (Heinz bodies) is considered unlikely, since we have demonstrated in this study that Heinz bodies cannot be visualized in unstained preparations, do not reveal the same granular structure, and do not contain significant amounts of iron above background. The occurrence of intracellular ferritin is discussed in light of current concepts of iron transport and storage during erythropoiesis.", "contents": "Stereo-electron microscopy and energy-dispersive x-ray analysis of avian reticulocytes. Utilizing the techniques of stereo-electron microscopy (stereo-EM) and energy-dispersive x-ray analysis (EDX), we have studied aspects of the ultrastructure of avian reticulocytes. Stereo-EM of thin sections (0.10 to 0.25 mum thick) stained with uranyl and lead revealed the three-dimensional arrangement of 25 nm chromatin fibers on the tangential surfaces of nuclei. Use of the Bernhard staining procedure in combination with stereo-EM permitted a three-dimensional view of the interchromatin spaces and channels leading to the nuclear pores, and of cytoplasmic polyribosomes. With either staining technique we frequently encountered in the cytoplasm clusters and paracrystalline arrays of electron-dense granules with granule diameters approximately 1/2 that of monomer ribosomes. These granules were highly electron-dense in unstained specimens and have been identified as intracellular ferritin on the basis of the similarity of their ultrastructural morphology to that of horse spleen ferritin and their high content of iron as determined by EDX. The possibility that these granules represent toxic products of phenylhydrazine treatment (Heinz bodies) is considered unlikely, since we have demonstrated in this study that Heinz bodies cannot be visualized in unstained preparations, do not reveal the same granular structure, and do not contain significant amounts of iron above background. The occurrence of intracellular ferritin is discussed in light of current concepts of iron transport and storage during erythropoiesis."}
{"id": "PMID:488124", "title": "The effects of nicotine on cell division of HeLa cells.", "content": "A time lapse cinemicrographic study shows that, at low concentrations, nicotine can speed up cytokinesis and, at high concentrations, prolong the duration of metaphase in HeLa cells. Based upon the known effects of the alkaloid in other systems, it appears that it may stimulate microfilament activity and inhibit microtubule function by increasing the level of cytosolic calcium ions.", "contents": "The effects of nicotine on cell division of HeLa cells. A time lapse cinemicrographic study shows that, at low concentrations, nicotine can speed up cytokinesis and, at high concentrations, prolong the duration of metaphase in HeLa cells. Based upon the known effects of the alkaloid in other systems, it appears that it may stimulate microfilament activity and inhibit microtubule function by increasing the level of cytosolic calcium ions."}
{"id": "PMID:488125", "title": "Cylindrical laminated bodies in nickel-subsulphide-induced rhabdomyosarcoma in rabbits.", "content": "The induction of rabbit rhabdomyosarcoma was obtained after intramuscular implantation of a large quantity of very pure nickel subsulphide, though until the present time the rabbit was considered refractory to Ni3S2 tumorigenesis. These tumors are similar to those induced in rats under the same conditions. Four different cell types were observed: small polygonal cells, small elongated cells, giant cells, and mature myofibers. Electron microscopy reveals a complete disorientation of myofibrils in mature myoblasts. Giant cells appear by pluripolar endomitosis and always contain myofibrillar structures, but M-lines and Z-lines are not present in these cells. Cylindrical laminated bodies were observed very often in all four cell types. They are formed of 4 nm fibrils arranged in crossed position in each lamella. Some of these paracrystalline structures were also observed in nuclei. The laminated bodies are considered to be abnormal formations of contractile proteins produced during tumoral myofibrillar differentiation.", "contents": "Cylindrical laminated bodies in nickel-subsulphide-induced rhabdomyosarcoma in rabbits. The induction of rabbit rhabdomyosarcoma was obtained after intramuscular implantation of a large quantity of very pure nickel subsulphide, though until the present time the rabbit was considered refractory to Ni3S2 tumorigenesis. These tumors are similar to those induced in rats under the same conditions. Four different cell types were observed: small polygonal cells, small elongated cells, giant cells, and mature myofibers. Electron microscopy reveals a complete disorientation of myofibrils in mature myoblasts. Giant cells appear by pluripolar endomitosis and always contain myofibrillar structures, but M-lines and Z-lines are not present in these cells. Cylindrical laminated bodies were observed very often in all four cell types. They are formed of 4 nm fibrils arranged in crossed position in each lamella. Some of these paracrystalline structures were also observed in nuclei. The laminated bodies are considered to be abnormal formations of contractile proteins produced during tumoral myofibrillar differentiation."}
{"id": "PMID:488128", "title": "Go and Go,2 cells as identified by their chromatin pattern in dormant and proliferating meristems.", "content": "Microdensitometry at different density thresholds of Feulgen-stained nuclei shows that both 2C and 4C dormant cells of the unsprouted root of Allium cepa L. bulbs have a smaller nuclear volume and a larger amount of dense chromatin than their counterpart G1 and G2 cells of the proliferating root. The analysis seems to differentiate between cells in the proliferate compartment from those in the non-proliferative compartment (Go and Go,2 cells).", "contents": "Go and Go,2 cells as identified by their chromatin pattern in dormant and proliferating meristems. Microdensitometry at different density thresholds of Feulgen-stained nuclei shows that both 2C and 4C dormant cells of the unsprouted root of Allium cepa L. bulbs have a smaller nuclear volume and a larger amount of dense chromatin than their counterpart G1 and G2 cells of the proliferating root. The analysis seems to differentiate between cells in the proliferate compartment from those in the non-proliferative compartment (Go and Go,2 cells)."}
{"id": "PMID:488129", "title": "Effects of oxybarbiturates on the germination behaviour of Impatiens bolstii pollen.", "content": "Swelling of and cytoplasmic streaming within the grain, germination pore expansion, and pollen tube emergence, in that order, constitute the normal germination pattern of Impatiens holstii pollen grains. This normal sequence of events becomes manifest when the pollen grains are placed on water-agar, barbituric acid-agar, and on barbital-agar media whereas this sequence of events is interfered with when the pollen grains are incubated on amytal-agar or on seconal-agar media. Evidence is presented which demonstrates that the normal sequence of events which make up the normal germination pattern of Impatiens holstii pollen grains can be separated from each other by varying the concentrations of the oxybarbiturates amytal and seconal used. Evidence is also presented which indicates that the actions of barbituric acid and the oxybarbiturates barbital, amytal, and seconal on Impatiens holstii pollen germination behaviour correlate well with certain aspects of the pharmacological literature concerning the actions of these organic compounds on the central nervous system (CNS).", "contents": "Effects of oxybarbiturates on the germination behaviour of Impatiens bolstii pollen. Swelling of and cytoplasmic streaming within the grain, germination pore expansion, and pollen tube emergence, in that order, constitute the normal germination pattern of Impatiens holstii pollen grains. This normal sequence of events becomes manifest when the pollen grains are placed on water-agar, barbituric acid-agar, and on barbital-agar media whereas this sequence of events is interfered with when the pollen grains are incubated on amytal-agar or on seconal-agar media. Evidence is presented which demonstrates that the normal sequence of events which make up the normal germination pattern of Impatiens holstii pollen grains can be separated from each other by varying the concentrations of the oxybarbiturates amytal and seconal used. Evidence is also presented which indicates that the actions of barbituric acid and the oxybarbiturates barbital, amytal, and seconal on Impatiens holstii pollen germination behaviour correlate well with certain aspects of the pharmacological literature concerning the actions of these organic compounds on the central nervous system (CNS)."}
{"id": "PMID:488130", "title": "Helically twisted filaments in giant neurons of a whip spider.", "content": "In giant neurons of whip spider legs several filament types are detectable: filaments of 5 to 6 nm thickness as dense masses within the soma of the neuron, an intermediate-sized filament type limited to the dendritic processes forming irregularly wound bundles and finally twisted double filaments in the soma as well as in peripheral regions. The latter are usually aggregated in paracristalloid lattices of different length and diameter.", "contents": "Helically twisted filaments in giant neurons of a whip spider. In giant neurons of whip spider legs several filament types are detectable: filaments of 5 to 6 nm thickness as dense masses within the soma of the neuron, an intermediate-sized filament type limited to the dendritic processes forming irregularly wound bundles and finally twisted double filaments in the soma as well as in peripheral regions. The latter are usually aggregated in paracristalloid lattices of different length and diameter."}
{"id": "PMID:488131", "title": "Effect of other drugs and chemicals on the degradation of aspirin in vitro: possible extrapolation to in vivo metabolism of aspirin.", "content": "To assess the possible influence of other drugs and chemicals on the metabolic degradation of aspirin in man, their effect on the serum aspirin esterase activity was determined in vitro. The activation or inhibition of the enzyme as observed with these compounds suggest the possibility that simultaneous ingestion of these drugs with aspirin may influence the pharmacology and toxicity of the analgesic.", "contents": "Effect of other drugs and chemicals on the degradation of aspirin in vitro: possible extrapolation to in vivo metabolism of aspirin. To assess the possible influence of other drugs and chemicals on the metabolic degradation of aspirin in man, their effect on the serum aspirin esterase activity was determined in vitro. The activation or inhibition of the enzyme as observed with these compounds suggest the possibility that simultaneous ingestion of these drugs with aspirin may influence the pharmacology and toxicity of the analgesic."}
{"id": "PMID:488132", "title": "The metabolism of saddamine in man: the fate of the benzylamine moiety of saddamine.", "content": "The metabolism of 14C-saddamine radiolabelled in the benzylamine moiety has been investigated in man after oral administration. 14C-Hippuric acid was found to be the only radiolabelled urinary metabolite, the overall amount of which was equivalent to over 95% of the dose. The time taken for elimination of 50% of the dose in the urine was approximately five hours and the corresponding value for 14C-benzylamine hydrochloride was less than one hour.", "contents": "The metabolism of saddamine in man: the fate of the benzylamine moiety of saddamine. The metabolism of 14C-saddamine radiolabelled in the benzylamine moiety has been investigated in man after oral administration. 14C-Hippuric acid was found to be the only radiolabelled urinary metabolite, the overall amount of which was equivalent to over 95% of the dose. The time taken for elimination of 50% of the dose in the urine was approximately five hours and the corresponding value for 14C-benzylamine hydrochloride was less than one hour."}
{"id": "PMID:488133", "title": "Pharmacokinetic parameters of 14C oxithiopurinol in rat.", "content": "The study with labelled compound has shown that in rat, as in man, thiopurinol is primarily and rapidly converted into oxithiopurinol. More than 80% of the radioactivity of plasma was identified as oxithiopurinol less than 10 minutes after intravenous or oral administration. The parameters of oxithiopurinol were estimated from the decline of radioactivity in blood from 5 minutes to 8 hours after intravenous administration of 4 microCi/100 g of thiopurinol (8 animals) or oxithiopurinol (5 animals). Half lives of oxithiopurinol t 1/2 (beta) were 2.52 +/- 0.78 hr and 2.90 +/- 1.00 hr and clearance 2.00 +/- 0.36 ml/min. and 3.38 +/- 0.69 respectively. Estimate of renal clearance for oxithiopurinol in rat was 4,17 ml/min. The rapidity of hydroxylation and of elimination of oxithiopurinol might be assumed to be the cause of the apparent ineffectiveness of thiopurinol in vivo on xanthine oxidase activity.", "contents": "Pharmacokinetic parameters of 14C oxithiopurinol in rat. The study with labelled compound has shown that in rat, as in man, thiopurinol is primarily and rapidly converted into oxithiopurinol. More than 80% of the radioactivity of plasma was identified as oxithiopurinol less than 10 minutes after intravenous or oral administration. The parameters of oxithiopurinol were estimated from the decline of radioactivity in blood from 5 minutes to 8 hours after intravenous administration of 4 microCi/100 g of thiopurinol (8 animals) or oxithiopurinol (5 animals). Half lives of oxithiopurinol t 1/2 (beta) were 2.52 +/- 0.78 hr and 2.90 +/- 1.00 hr and clearance 2.00 +/- 0.36 ml/min. and 3.38 +/- 0.69 respectively. Estimate of renal clearance for oxithiopurinol in rat was 4,17 ml/min. The rapidity of hydroxylation and of elimination of oxithiopurinol might be assumed to be the cause of the apparent ineffectiveness of thiopurinol in vivo on xanthine oxidase activity."}
{"id": "PMID:488134", "title": "In vivo studied on the metabolism of dibenzo [c,f]-[1,2] diazepine.", "content": "The in vivo metabolism of dibenzo [c,f]-[1,2] diazepine (I) was studied after i.p. administration to rats, in order to observe the influence of the heteroatom on the metabolic pathway. Metabolites were separated by chromatography and their structures elucidated by mass spectrometry, by both gas chromatography coupled with mass spectrometry and the direct inlet system. Six metabolites were identified, some of them by direct comparison with authentic samples.", "contents": "In vivo studied on the metabolism of dibenzo [c,f]-[1,2] diazepine. The in vivo metabolism of dibenzo [c,f]-[1,2] diazepine (I) was studied after i.p. administration to rats, in order to observe the influence of the heteroatom on the metabolic pathway. Metabolites were separated by chromatography and their structures elucidated by mass spectrometry, by both gas chromatography coupled with mass spectrometry and the direct inlet system. Six metabolites were identified, some of them by direct comparison with authentic samples."}
{"id": "PMID:488136", "title": "Prednisolone absorption in coeliac disease.", "content": "The pharmacokinetic disposition of prednisolone was studied following oral administration (10 mg) to eight patients with untreated coeliac disease of mild or moderate severity, seven coeliac patients on a strict gluten-free diet and ten normal subjects. No significant differences were shown in the peak prednisolone level, the time of the peak level, the area under the concentration versus time plot, the plasma half-life and the 24 h urinary recovery of prednisolone in the three subject groups. There was however considerable variability within each group. It is concluded that the presence of coeliac disease does not significantly alter the absorption or elimination of prednisolone.", "contents": "Prednisolone absorption in coeliac disease. The pharmacokinetic disposition of prednisolone was studied following oral administration (10 mg) to eight patients with untreated coeliac disease of mild or moderate severity, seven coeliac patients on a strict gluten-free diet and ten normal subjects. No significant differences were shown in the peak prednisolone level, the time of the peak level, the area under the concentration versus time plot, the plasma half-life and the 24 h urinary recovery of prednisolone in the three subject groups. There was however considerable variability within each group. It is concluded that the presence of coeliac disease does not significantly alter the absorption or elimination of prednisolone."}
{"id": "PMID:488137", "title": "The fate of adenosine monophosphate in the rat after a single oral administration. Influence of a simultaneous treatment by papaverine.", "content": "The fate in the organism of Adenosine Monophosphate after a single oral administration of 10 mg/kg, was studied in the Rat, using 14C-labelled AMP. This paper deals more specially with the study of the plasmatic kinetics of AMP, since we proved at first that the AMP molecule goes intact through the intestinal barrier into the blood. The effects on these kinetics of a joint administration of Papaverine are considered.", "contents": "The fate of adenosine monophosphate in the rat after a single oral administration. Influence of a simultaneous treatment by papaverine. The fate in the organism of Adenosine Monophosphate after a single oral administration of 10 mg/kg, was studied in the Rat, using 14C-labelled AMP. This paper deals more specially with the study of the plasmatic kinetics of AMP, since we proved at first that the AMP molecule goes intact through the intestinal barrier into the blood. The effects on these kinetics of a joint administration of Papaverine are considered."}
{"id": "PMID:488138", "title": "CT evaluation in patients with transient ischemic attack. Correlation between clinical and angiographic findings.", "content": "35 patients clinically diagnosed with transient ischemic attack (TIA) and 15 patients clinically diagnosed with reversible ischemic neurological deficits (RIND) were examined by computerized tomography (CT). 34% of TIA patients showed positive CT scans consisting of small hypodenase areas: 60% of RIND patients had larger lesions. In 20% of the overall group the only pathological finding consisted of unilateral focal atophy or ventricular asymmetry. Correlation between angiographically demonstrated atherosclerotic lesions and positive CT was high. On the contrary, no correlation was found between either the risk factors or the natural history of ischemic attack and positive CT findings.", "contents": "CT evaluation in patients with transient ischemic attack. Correlation between clinical and angiographic findings. 35 patients clinically diagnosed with transient ischemic attack (TIA) and 15 patients clinically diagnosed with reversible ischemic neurological deficits (RIND) were examined by computerized tomography (CT). 34% of TIA patients showed positive CT scans consisting of small hypodenase areas: 60% of RIND patients had larger lesions. In 20% of the overall group the only pathological finding consisted of unilateral focal atophy or ventricular asymmetry. Correlation between angiographically demonstrated atherosclerotic lesions and positive CT was high. On the contrary, no correlation was found between either the risk factors or the natural history of ischemic attack and positive CT findings."}
{"id": "PMID:488139", "title": "Does anoxemia play a role in the effects of neonatal seizures on brain growth? An experimental study in the rat.", "content": "Groups of 4 Wistar rat littermates of matched sex and weight received the following daily treatment between the ages of 2 and 11 days: handling (untreated controls, UC), succinylcholine paralysis and ventilation with 100% O2 (respirator control, RC), electroconvulsive seizures (ECS) and ECS while paralyzed and ventilated with O2 (respirator seizures, RS). Analysis of heart blood O2 content showed that mechanical ventilation with 100% O2 effectively prevented the anoxemia observed during convulsive seizures. In the ECS and RS groups, several behavioral milestones (e.g. swimming) matured later than they did in either control group (UC, RC). No difference was observed between the two groups of seizure-treated rats. At the age of 30 days, both ECS and RS groups had smaller brains with a reduced DNA, RNA, protein and cholesterol content in both forebrain and hindbrain, suggesting that seizures curtailed the number of brain cells. The lack of any difference between the two seizure groups suggests that at least part of the adverse effects of experimental neonatal seizures on brain and behaviour are independent of anoxemia.", "contents": "Does anoxemia play a role in the effects of neonatal seizures on brain growth? An experimental study in the rat. Groups of 4 Wistar rat littermates of matched sex and weight received the following daily treatment between the ages of 2 and 11 days: handling (untreated controls, UC), succinylcholine paralysis and ventilation with 100% O2 (respirator control, RC), electroconvulsive seizures (ECS) and ECS while paralyzed and ventilated with O2 (respirator seizures, RS). Analysis of heart blood O2 content showed that mechanical ventilation with 100% O2 effectively prevented the anoxemia observed during convulsive seizures. In the ECS and RS groups, several behavioral milestones (e.g. swimming) matured later than they did in either control group (UC, RC). No difference was observed between the two groups of seizure-treated rats. At the age of 30 days, both ECS and RS groups had smaller brains with a reduced DNA, RNA, protein and cholesterol content in both forebrain and hindbrain, suggesting that seizures curtailed the number of brain cells. The lack of any difference between the two seizure groups suggests that at least part of the adverse effects of experimental neonatal seizures on brain and behaviour are independent of anoxemia."}
{"id": "PMID:488140", "title": "Post-convulsive spinal epidural haematoma in ankylosing spondylitis.", "content": "A case of post-convulsive spinal spidural haematoma in a patient with ankylosing spondylitis is presented. As acute tetraplegia developed, surgery was performed with finding of blood clots in the extradural space from C6 to D8. Lethal evolution due to late referral to medical care stresses the need for prompt decompression of the injured spine.", "contents": "Post-convulsive spinal epidural haematoma in ankylosing spondylitis. A case of post-convulsive spinal spidural haematoma in a patient with ankylosing spondylitis is presented. As acute tetraplegia developed, surgery was performed with finding of blood clots in the extradural space from C6 to D8. Lethal evolution due to late referral to medical care stresses the need for prompt decompression of the injured spine."}
{"id": "PMID:488141", "title": "Evoked potentials in neuronal ceroid lipofuscinosis.", "content": "Nerve conduction, EEG, visual evoked potentials, electroretinograms and somatosensory evoked potentials were investigated in 3 children with the Bielschowsky-Jansky-type and in 1 child diagnosed as Spielmeyer-Vogt-type of neuronal ceroid lipofuscinosis. Electroretinographic responses were abolished in all of them. Electroencephalograms showed high amplitude, irregular delta-theta activity and spike- or polyspike-wave discharges without localized preponderance. As a characteristic feature for the Bielschowsky-Jansky type grossly enlarged evoked responses to single light flashes were recorded. Somatosensory evoked potentials were increased in amplitude in 2 patients. Myoclonic jerks of the pyramidal type could be elicited by electrical stimuli to the median nerve. The possibility to differentiate certain neurometabolic disorders of childhood by simple electrophysiological parameters is discussed.", "contents": "Evoked potentials in neuronal ceroid lipofuscinosis. Nerve conduction, EEG, visual evoked potentials, electroretinograms and somatosensory evoked potentials were investigated in 3 children with the Bielschowsky-Jansky-type and in 1 child diagnosed as Spielmeyer-Vogt-type of neuronal ceroid lipofuscinosis. Electroretinographic responses were abolished in all of them. Electroencephalograms showed high amplitude, irregular delta-theta activity and spike- or polyspike-wave discharges without localized preponderance. As a characteristic feature for the Bielschowsky-Jansky type grossly enlarged evoked responses to single light flashes were recorded. Somatosensory evoked potentials were increased in amplitude in 2 patients. Myoclonic jerks of the pyramidal type could be elicited by electrical stimuli to the median nerve. The possibility to differentiate certain neurometabolic disorders of childhood by simple electrophysiological parameters is discussed."}
{"id": "PMID:488142", "title": "Diagnostic validity for different components of pattern shift visual evoked potentials in multiple sclerosis.", "content": "Visual evoked potentials, elicited by checkerboard-pattern reversal were recorded from multiple sclerosis patients with different probabilities of diagnosis. Latencies and amplitudes of the different components were analyzed for diagnostic validity. It was demonstrated that the latency of the main positive peak (P2) was the most sensitive parameter. But in a few cases determination of P2 latency and interocular difference of this peak alone failed to detect involvement of the visual system. Additional evaluation of absolute latencies, asymmetries in latency and amplitudes of other components would give evidence in predicting abnormalities of the visual pathways.", "contents": "Diagnostic validity for different components of pattern shift visual evoked potentials in multiple sclerosis. Visual evoked potentials, elicited by checkerboard-pattern reversal were recorded from multiple sclerosis patients with different probabilities of diagnosis. Latencies and amplitudes of the different components were analyzed for diagnostic validity. It was demonstrated that the latency of the main positive peak (P2) was the most sensitive parameter. But in a few cases determination of P2 latency and interocular difference of this peak alone failed to detect involvement of the visual system. Additional evaluation of absolute latencies, asymmetries in latency and amplitudes of other components would give evidence in predicting abnormalities of the visual pathways."}
{"id": "PMID:488143", "title": "Capillary circulation and morphology in Duchenne muscular dystrophy.", "content": "Muscle blood flow (MBF) and capillary diffusion capacity (CDC) were determined in 8 patients with Duchenne muscular dystrophy and 5 age-matched control males by measuring the simultaneous clearance of 133Xe and 131I-. MBF was significantly decreased in older patients with severe dystrophy: 35.1 +/- 5.8 ml/100 g/min in 4 advanced Duchenne patients and 45.8 +/- 6.5 ml/100 g/min in controls (p less than 0.005). MBF in 4 early cases did not differ from controls (44.0 +/- 7.9 ml/100 g/min). There was no significant difference in CDC in Duchenne patients and controls. No structural abnormalities in muscle microvasculature were found by light microscopy. In the electron microscope, the Duchenne basement membranes had duplicated or multiple layers of electron-dense material in 63.9% of muscle capillaries while only 6.6% of the control capillaries showed this. These results do not support an ischemic etiology of Duchenne muscular dystrophy.", "contents": "Capillary circulation and morphology in Duchenne muscular dystrophy. Muscle blood flow (MBF) and capillary diffusion capacity (CDC) were determined in 8 patients with Duchenne muscular dystrophy and 5 age-matched control males by measuring the simultaneous clearance of 133Xe and 131I-. MBF was significantly decreased in older patients with severe dystrophy: 35.1 +/- 5.8 ml/100 g/min in 4 advanced Duchenne patients and 45.8 +/- 6.5 ml/100 g/min in controls (p less than 0.005). MBF in 4 early cases did not differ from controls (44.0 +/- 7.9 ml/100 g/min). There was no significant difference in CDC in Duchenne patients and controls. No structural abnormalities in muscle microvasculature were found by light microscopy. In the electron microscope, the Duchenne basement membranes had duplicated or multiple layers of electron-dense material in 63.9% of muscle capillaries while only 6.6% of the control capillaries showed this. These results do not support an ischemic etiology of Duchenne muscular dystrophy."}
{"id": "PMID:488144", "title": "Ephaptic recurrent response in man.", "content": "The antidromic impulse associated to the ephaptic response can produce a recurrent wave (Fe). Its latency, which is longer than that of the F wave, will augment if the site of stimulation is remote from the muscle. It is therefore a direct-indirect response. The 'Fe' wave is similar to the 'S' response described by Thorne, but the gamma-loop is not implicated. It can be misinterpreted as a desynchronized F wave.", "contents": "Ephaptic recurrent response in man. The antidromic impulse associated to the ephaptic response can produce a recurrent wave (Fe). Its latency, which is longer than that of the F wave, will augment if the site of stimulation is remote from the muscle. It is therefore a direct-indirect response. The 'Fe' wave is similar to the 'S' response described by Thorne, but the gamma-loop is not implicated. It can be misinterpreted as a desynchronized F wave."}
{"id": "PMID:488145", "title": "Ephaptic response in man.", "content": "Following the direct M response, peripheral nerve stimulation frequently evokes a second wave which shows all characteristics of an ephaptic response. The antidromic impulse or back response, which is always associated, is detected by a double stimulation technique. The ephaptic response seems not to be related to any specific nerve or muscle pathology, as it is also observed in normal subjects. It is therefore important to differentiate the ephaptic response from a late potential or a double discharge, the latter two having pathological significance.", "contents": "Ephaptic response in man. Following the direct M response, peripheral nerve stimulation frequently evokes a second wave which shows all characteristics of an ephaptic response. The antidromic impulse or back response, which is always associated, is detected by a double stimulation technique. The ephaptic response seems not to be related to any specific nerve or muscle pathology, as it is also observed in normal subjects. It is therefore important to differentiate the ephaptic response from a late potential or a double discharge, the latter two having pathological significance."}
{"id": "PMID:488146", "title": "Lithium therapy in cluster headache.", "content": "The authors used lithium carbonate in the treatment of 15 patients with cluster headache (chronic and episodice form). Disappearance of attacks was achieved in 5 patients (1 chronic and 4 episodic), significant improvement in 5 (4 chronic and 1 episodic) and in 4 cases (2 chronic and 2 episodic) treatment was ineffective.", "contents": "Lithium therapy in cluster headache. The authors used lithium carbonate in the treatment of 15 patients with cluster headache (chronic and episodice form). Disappearance of attacks was achieved in 5 patients (1 chronic and 4 episodic), significant improvement in 5 (4 chronic and 1 episodic) and in 4 cases (2 chronic and 2 episodic) treatment was ineffective."}
{"id": "PMID:488147", "title": "Free fatty acid and glucose metabolism during hypothermic perfusion of canine kidneys.", "content": "Canine kidneys were subjected to continuous nonpulsatile perfusion using 200 ml of a perfusate containing 50 g/l albumin. When optimal oxygenation was achieved, perfusate K+ contents were unchanged for 24 h, indicating adequate membrane function but tended to increase thereafter. Lowered oxygen pressures resulted in significant cellular K+ loss during the first hours of perfusion. During oxygenated perfusion, glucose and free fatty acids (FFA) were oxidized in considerable amounts with a preferential consumption of octanoate. A capacity for long-chain FFA oxidation became obvious when the octanoate had been used up, but the amount of these FFA in the perfusate depended preferentially on FFA being liberated from tissue lipids during the 1st day of perfusion. Glucose consumption rates were highest during the first 2 days of perfusion but the subsequent reduction of the metabolic rate was not accompanied by an accumulation of lactate. Thus medium-chain FFA and glucose should be supplied to the continuously perfused kidney in hypothermia and optimal oxygenation of the perfusate should be guaranteed. However, it seems to be unnecessary to supply exogenous long-chain FFA.", "contents": "Free fatty acid and glucose metabolism during hypothermic perfusion of canine kidneys. Canine kidneys were subjected to continuous nonpulsatile perfusion using 200 ml of a perfusate containing 50 g/l albumin. When optimal oxygenation was achieved, perfusate K+ contents were unchanged for 24 h, indicating adequate membrane function but tended to increase thereafter. Lowered oxygen pressures resulted in significant cellular K+ loss during the first hours of perfusion. During oxygenated perfusion, glucose and free fatty acids (FFA) were oxidized in considerable amounts with a preferential consumption of octanoate. A capacity for long-chain FFA oxidation became obvious when the octanoate had been used up, but the amount of these FFA in the perfusate depended preferentially on FFA being liberated from tissue lipids during the 1st day of perfusion. Glucose consumption rates were highest during the first 2 days of perfusion but the subsequent reduction of the metabolic rate was not accompanied by an accumulation of lactate. Thus medium-chain FFA and glucose should be supplied to the continuously perfused kidney in hypothermia and optimal oxygenation of the perfusate should be guaranteed. However, it seems to be unnecessary to supply exogenous long-chain FFA."}
{"id": "PMID:488148", "title": "Healing of enterotomies with preexisting peritonitis. An experimental study in guinea pigs.", "content": "The question of whether the presence of peritonitis has any influence upon the process of healing and the load capacity of a standard enterotomy was studied in guinea pigs. Laparotomy was performed 4 h after induction of peritonitis by a standardized method. Fecal peritonitis could be confirmed clinically, histologically and bacteriogically in all cases. A standardized enterotomy was carried out. The animals were killed 3 or 5 days later, anastomoses inspected, and the intraluminal pressures for anastomotic rupture measured. Comparison with a control group showed: (1) there can be normal healing of an enterotomy even with coexisting peritonitis; (2) anastomotic breakdown takes place at the same pressure as in the control group (p less than 0.01), and (3) primary lethality is significantly higher in cases operated in the presence of peritonitis (33 vs. 20%).", "contents": "Healing of enterotomies with preexisting peritonitis. An experimental study in guinea pigs. The question of whether the presence of peritonitis has any influence upon the process of healing and the load capacity of a standard enterotomy was studied in guinea pigs. Laparotomy was performed 4 h after induction of peritonitis by a standardized method. Fecal peritonitis could be confirmed clinically, histologically and bacteriogically in all cases. A standardized enterotomy was carried out. The animals were killed 3 or 5 days later, anastomoses inspected, and the intraluminal pressures for anastomotic rupture measured. Comparison with a control group showed: (1) there can be normal healing of an enterotomy even with coexisting peritonitis; (2) anastomotic breakdown takes place at the same pressure as in the control group (p less than 0.01), and (3) primary lethality is significantly higher in cases operated in the presence of peritonitis (33 vs. 20%)."}
{"id": "PMID:488149", "title": "Homologous blood syndrome in dogs. Effect of erythrocyte and platelet antibodies.", "content": "Isovolemic perfusion of 50% of the recipient circulating blood volume was carried out in intact mongrel dogs using whole donor blood as well as plasma or platelet suspensions in saline. Perfusion against the background of circulating red blood cells (RBC) or platelets antibodies in the donor or in the recipient was followed by severe and sometimes fatal homologous blood syndrome in a majority of the dogs. Weak and short-term reactions were seen in a few dogs if red blood cells and platelet antibodies were absent.", "contents": "Homologous blood syndrome in dogs. Effect of erythrocyte and platelet antibodies. Isovolemic perfusion of 50% of the recipient circulating blood volume was carried out in intact mongrel dogs using whole donor blood as well as plasma or platelet suspensions in saline. Perfusion against the background of circulating red blood cells (RBC) or platelets antibodies in the donor or in the recipient was followed by severe and sometimes fatal homologous blood syndrome in a majority of the dogs. Weak and short-term reactions were seen in a few dogs if red blood cells and platelet antibodies were absent."}
{"id": "PMID:488150", "title": "Repreoducibility of a method for a graded stenosis in tubes and vessels of small calibres. An in vitro and in vivo experiment.", "content": "The reproducibility of a previously described method for graded stenosis has been tested in a model experiment and by repeated stenosis of the portal vein in rats. In the model experiment, the method was tested in terms of flow variations; in the animal experiment, in terms of prestenotic pressure variations. In the model experiment, the coefficient of variation in repeated stenosis was less than 3%. In the animal experiment, repeated stenosis constantly produced the same high level of pressure. The present method is discussed in relation to a theoretical optimal standard and found to be satisfactory.", "contents": "Repreoducibility of a method for a graded stenosis in tubes and vessels of small calibres. An in vitro and in vivo experiment. The reproducibility of a previously described method for graded stenosis has been tested in a model experiment and by repeated stenosis of the portal vein in rats. In the model experiment, the method was tested in terms of flow variations; in the animal experiment, in terms of prestenotic pressure variations. In the model experiment, the coefficient of variation in repeated stenosis was less than 3%. In the animal experiment, repeated stenosis constantly produced the same high level of pressure. The present method is discussed in relation to a theoretical optimal standard and found to be satisfactory."}
{"id": "PMID:488151", "title": "Prehepatic portal hypertension in the rat. Immediate and long-term effects on portal vein and aortic pressure of a graded portal vein stenosis, followed by occlusion of the portal vein and spleno-renal collaterals.", "content": "The creation of a portal vein stenosis with a diameter of 1.2 mm in Wistar rats resulted in a sustained elevation of the portal vein pressure to approximately twice the control value lasting for at least 8 weeks. The alterations in portal vein pressure following occlusion of the stenosed portal vein and splenorenal collaterals, either separately or in combination, indicate that a considerable quantity of portal blood is diverted into the inferior caval vein through the splenorenal collaterals, and that the development of these collaterals occurs during the first 3-4 weeks after the establishment of the stenosis. The concomitant reduction in aortic pressure and increase in heart rate following total occlusion of the portal vein were most pronounced during the first weeks after stenosis, and were probably due to diminished venous return to the heart.", "contents": "Prehepatic portal hypertension in the rat. Immediate and long-term effects on portal vein and aortic pressure of a graded portal vein stenosis, followed by occlusion of the portal vein and spleno-renal collaterals. The creation of a portal vein stenosis with a diameter of 1.2 mm in Wistar rats resulted in a sustained elevation of the portal vein pressure to approximately twice the control value lasting for at least 8 weeks. The alterations in portal vein pressure following occlusion of the stenosed portal vein and splenorenal collaterals, either separately or in combination, indicate that a considerable quantity of portal blood is diverted into the inferior caval vein through the splenorenal collaterals, and that the development of these collaterals occurs during the first 3-4 weeks after the establishment of the stenosis. The concomitant reduction in aortic pressure and increase in heart rate following total occlusion of the portal vein were most pronounced during the first weeks after stenosis, and were probably due to diminished venous return to the heart."}
{"id": "PMID:488157", "title": "Lysolecithin analogs as adjuvants in delayed-type hypersensitivity in mice. II. Studies on the mode of action.", "content": "Lysolecithin analogs (LLA) possess adjuvant activity in delayed-type hypersensitivity (DTH). To elucidate their mode of action, we studied the influence of LLA on the recruitment of hemopoietic progenitor cells and on the induction and transfer of DTH. Whereas the number and composition of colony-forming cells (CFC) in the bone marrow remained unaltered in LLA-treated mice, the number of CFC in the spleen was augmented severalfold, and the content of macrophage progenitors was remarkably increased. DTH was induced with macrophages from peritoneal cells (PC) pulsed with keyhole limpet hemocyanin (KLH). A more marked DTH response was obtained with KLH-pulsed PC from LLA-treated mice than from mice given phosphate-buffered saline (PBS). On the other hand, there was no difference in the DTH response following adoptive transfer of sensitized cells to naive mice pretreated either with PBS or LLA. It is concluded that the adjuvant effect of LLA can be accounted for partly in terms of their effects on macrophage progenitors and partly in terms of a more effective T cell activation by antigens associated with LLA-derived macrophages.", "contents": "Lysolecithin analogs as adjuvants in delayed-type hypersensitivity in mice. II. Studies on the mode of action. Lysolecithin analogs (LLA) possess adjuvant activity in delayed-type hypersensitivity (DTH). To elucidate their mode of action, we studied the influence of LLA on the recruitment of hemopoietic progenitor cells and on the induction and transfer of DTH. Whereas the number and composition of colony-forming cells (CFC) in the bone marrow remained unaltered in LLA-treated mice, the number of CFC in the spleen was augmented severalfold, and the content of macrophage progenitors was remarkably increased. DTH was induced with macrophages from peritoneal cells (PC) pulsed with keyhole limpet hemocyanin (KLH). A more marked DTH response was obtained with KLH-pulsed PC from LLA-treated mice than from mice given phosphate-buffered saline (PBS). On the other hand, there was no difference in the DTH response following adoptive transfer of sensitized cells to naive mice pretreated either with PBS or LLA. It is concluded that the adjuvant effect of LLA can be accounted for partly in terms of their effects on macrophage progenitors and partly in terms of a more effective T cell activation by antigens associated with LLA-derived macrophages."}
{"id": "PMID:488158", "title": "Ischemic-induced alterations in cardiac sensitivity to digitalis.", "content": "Intravenous infusion of acetylstrophanthidin to 6 dogs, after a 60 min left anterior descending coronary artery occlusion, was associated with a 43.0 +/- 10.5% decrease in the dose of digitalis needed to produce ventricular arrhythmias as compared to the pre-ischemic dose (97.5 +/- 8.0 microgram/kg). Reperfusion of the ischemic region for 2 h after a 90 min occlusion resulted in a 54.4 +/- 6.7% decrease in the arrhythmogenic dose. Direct intracoronary infusions of digitalis into the ischemic region, after a 90 min coronary occlusion followed by 2 h of reperfusion, was associated with a 47.7 +/- 6.4% decrease in the dose of digitalis needed to produce arrhythmias. The pre-ischemic (control) arrhythmogenic dose of digitalis via the intracoronary infusion method was 1.5 +/- 0.3 microgram/kg (mean +/- S.E.M. of 7 dogs). Sodium pump activity, estimated from the ouabain-sensitive 86Rb uptake in sodium-loaded ventricular slices, was significantly higher in slices obtained from the ischemic regions (6.84 +/- 0.30 nmoles 86Rb/mg dry wt. (mean +/- S.E.M.), than from the non-ischemic regions (3.43 +/- 0.64 nmoles 86Rb/mg dry wt.). Sensitivity of the sodium pump activity to the inhibitory effect of ouabain also was increased in the ischemic regions as indicated by a shift in the log dose--response curve to the left. Thus, it appears that there is an increase in myocardial sensitivity to the toxic effect of digitalis after temporary ischemia and it appears to be related to an increase in the sensitivity of the Na+,K+-ATPase or sodium pump to the inhibitory effect of digitalis.", "contents": "Ischemic-induced alterations in cardiac sensitivity to digitalis. Intravenous infusion of acetylstrophanthidin to 6 dogs, after a 60 min left anterior descending coronary artery occlusion, was associated with a 43.0 +/- 10.5% decrease in the dose of digitalis needed to produce ventricular arrhythmias as compared to the pre-ischemic dose (97.5 +/- 8.0 microgram/kg). Reperfusion of the ischemic region for 2 h after a 90 min occlusion resulted in a 54.4 +/- 6.7% decrease in the arrhythmogenic dose. Direct intracoronary infusions of digitalis into the ischemic region, after a 90 min coronary occlusion followed by 2 h of reperfusion, was associated with a 47.7 +/- 6.4% decrease in the dose of digitalis needed to produce arrhythmias. The pre-ischemic (control) arrhythmogenic dose of digitalis via the intracoronary infusion method was 1.5 +/- 0.3 microgram/kg (mean +/- S.E.M. of 7 dogs). Sodium pump activity, estimated from the ouabain-sensitive 86Rb uptake in sodium-loaded ventricular slices, was significantly higher in slices obtained from the ischemic regions (6.84 +/- 0.30 nmoles 86Rb/mg dry wt. (mean +/- S.E.M.), than from the non-ischemic regions (3.43 +/- 0.64 nmoles 86Rb/mg dry wt.). Sensitivity of the sodium pump activity to the inhibitory effect of ouabain also was increased in the ischemic regions as indicated by a shift in the log dose--response curve to the left. Thus, it appears that there is an increase in myocardial sensitivity to the toxic effect of digitalis after temporary ischemia and it appears to be related to an increase in the sensitivity of the Na+,K+-ATPase or sodium pump to the inhibitory effect of digitalis."}
{"id": "PMID:488159", "title": "Immobility induced by forced swimming in rats: effects of agents which modify central catecholamine and serotonin activity.", "content": "Rats were forced to swim in a restricted space will rapidly cease apparent attempts to escape and adopt a characteristic posture which we have termed \"immobility\". We show in previous experiments that immobility was reduced by a variety of antidepressant agents and thus suggested that the method could serve as a screening model for antidepressants. The present experiments showed that immobility was reduced by drugs which increase central dopaminergic and alpha-adrenergic activity but was less affected by drugs which act mainly on central serotonin. Conversely, immobility could be increased by drugs which diminish central catecholamine activity but not by drugs which inhibit central serotonin. It was concluded that immobility depended primarily on the activity of central catecholamines but that caution was required before ascribing immobility exclusively to activity within a single system.", "contents": "Immobility induced by forced swimming in rats: effects of agents which modify central catecholamine and serotonin activity. Rats were forced to swim in a restricted space will rapidly cease apparent attempts to escape and adopt a characteristic posture which we have termed \"immobility\". We show in previous experiments that immobility was reduced by a variety of antidepressant agents and thus suggested that the method could serve as a screening model for antidepressants. The present experiments showed that immobility was reduced by drugs which increase central dopaminergic and alpha-adrenergic activity but was less affected by drugs which act mainly on central serotonin. Conversely, immobility could be increased by drugs which diminish central catecholamine activity but not by drugs which inhibit central serotonin. It was concluded that immobility depended primarily on the activity of central catecholamines but that caution was required before ascribing immobility exclusively to activity within a single system."}
{"id": "PMID:488160", "title": "Effect of various antidepressant drugs on the spontaneous firing rate of locus coeruleus and dorsal raphe neurons of the rat.", "content": "The spontaneous firing rate of the noradrenergic neurons of the locus coeruleus and of the serotonergic neurons of the dorsal raphe was recorded with extracellular microelectrodes in chloral hydrate-anesthetized rats. A quantitative comparison of the effect of five tricyclic antidepressants, of tranylcypromine and of mianserin on the spontaneous activity of these two types of cells was performed. All drugs tested, except mianserin reduced the frequency of discharge of the noradrenergic neurons. Intravenous perfusion of the drugs allowed the doses required for inhibition of firing to 50% of the baseline rate (ID50) to be determined. Secondary aminated antidepressants (desipramine and nortriptyline) were more potent inhibitors than their tertiary aminated analogues (imipramine, chlorimipramine and amitriptyline). All drugs tested, except desipramine decreased the rate of firing of the serotonergic cells. In this case, the tertiary aminated antidepressants were much more potent than their secondary analogues. Mianserin was only active at very high doses. These results are in good agreement with the relative potencies of the tricyclic antidepressants for blocking the uptake of noradrenaline and serotonin into central and peripheral neurons.", "contents": "Effect of various antidepressant drugs on the spontaneous firing rate of locus coeruleus and dorsal raphe neurons of the rat. The spontaneous firing rate of the noradrenergic neurons of the locus coeruleus and of the serotonergic neurons of the dorsal raphe was recorded with extracellular microelectrodes in chloral hydrate-anesthetized rats. A quantitative comparison of the effect of five tricyclic antidepressants, of tranylcypromine and of mianserin on the spontaneous activity of these two types of cells was performed. All drugs tested, except mianserin reduced the frequency of discharge of the noradrenergic neurons. Intravenous perfusion of the drugs allowed the doses required for inhibition of firing to 50% of the baseline rate (ID50) to be determined. Secondary aminated antidepressants (desipramine and nortriptyline) were more potent inhibitors than their tertiary aminated analogues (imipramine, chlorimipramine and amitriptyline). All drugs tested, except desipramine decreased the rate of firing of the serotonergic cells. In this case, the tertiary aminated antidepressants were much more potent than their secondary analogues. Mianserin was only active at very high doses. These results are in good agreement with the relative potencies of the tricyclic antidepressants for blocking the uptake of noradrenaline and serotonin into central and peripheral neurons."}
{"id": "PMID:488161", "title": "Pre- and postsynaptic localization of central muscarinic receptors.", "content": "3H-Quinuclidinyl benzylate and dimethyl 14C-d-tubocurarine were used to localize muscarinic and nicotinic receptors in synaptosomal membranes of the rat cerebral cortex. The results obtained, after the action of different concentrations of Triton X-100, suggest that, while nicotinic receptors are postsynaptic, the muscarinic ones are both pre- and postsynaptic.", "contents": "Pre- and postsynaptic localization of central muscarinic receptors. 3H-Quinuclidinyl benzylate and dimethyl 14C-d-tubocurarine were used to localize muscarinic and nicotinic receptors in synaptosomal membranes of the rat cerebral cortex. The results obtained, after the action of different concentrations of Triton X-100, suggest that, while nicotinic receptors are postsynaptic, the muscarinic ones are both pre- and postsynaptic."}
{"id": "PMID:488162", "title": "Antagonism by droperidol of dopamine-induced relaxation in isolated dog arteries.", "content": "Dopamine caused a dose-related relaxation in helically cut strips of dog coronary and renal arteries treated with phenoxybenzamine andcontracted with prostaglandin F2 alpha. The dose-response curve to dopamine was shifted to the right by droperidol in concentrations above 3 X 10(-5) M. Adenosine-induced relaxations were not attenuated by droperidol. The dose--relaxation curve to isoproterenol was also shifted to the right by droperidol. Propranolol (10(-6) M) failed to significantly alter the dose response curve to dopamine, and in propranolol-treated preparations the antagonism by droperidol of dopamine actions was practically identical with that in control preparations. Droperidol appears to act as a reversible, surmountable antagonist to dopamine actions on dog arterial smooth muscles, and such evidence supports the hypothesis of specific dopamine receptors in dog arteries.", "contents": "Antagonism by droperidol of dopamine-induced relaxation in isolated dog arteries. Dopamine caused a dose-related relaxation in helically cut strips of dog coronary and renal arteries treated with phenoxybenzamine andcontracted with prostaglandin F2 alpha. The dose-response curve to dopamine was shifted to the right by droperidol in concentrations above 3 X 10(-5) M. Adenosine-induced relaxations were not attenuated by droperidol. The dose--relaxation curve to isoproterenol was also shifted to the right by droperidol. Propranolol (10(-6) M) failed to significantly alter the dose response curve to dopamine, and in propranolol-treated preparations the antagonism by droperidol of dopamine actions was practically identical with that in control preparations. Droperidol appears to act as a reversible, surmountable antagonist to dopamine actions on dog arterial smooth muscles, and such evidence supports the hypothesis of specific dopamine receptors in dog arteries."}
{"id": "PMID:488163", "title": "gamma-Glutamyl DOPA and gamma-glutamyl dopamine: effect on plasma glucose levels.", "content": "gamma-Glutamyl-L-3,4-dihydroxyphenylalanine (gamma-glutamyl DOPA) and gamma-glutamyldopamine (gamma-glutamyl DA) are kidney specific prodrugs. Their effect on plasma glucose levels in the rat was compared to that of L-DOPA and dopamine (DA) after a 30 min intravenous infusion. L-DOPA and DA induced hyperglycemia after 15 min of druginfusion. A more marked and protracted elevation of plasma glucose was observed after infusion of gamma-glutamyl DA. By gamma-glutamyl DOPA had no effect on plasma glucose levels in spite of the high accumulation of DA in the pancreas after this prodrug. Of the various dopamine produrgs studied only gamma-glutamyl DOPA was not hyperglycemic in doses that are known to increase renal plasma flow in the rat. A simplified new procedure for the synthesis of gamma-glutamyl DA is described.", "contents": "gamma-Glutamyl DOPA and gamma-glutamyl dopamine: effect on plasma glucose levels. gamma-Glutamyl-L-3,4-dihydroxyphenylalanine (gamma-glutamyl DOPA) and gamma-glutamyldopamine (gamma-glutamyl DA) are kidney specific prodrugs. Their effect on plasma glucose levels in the rat was compared to that of L-DOPA and dopamine (DA) after a 30 min intravenous infusion. L-DOPA and DA induced hyperglycemia after 15 min of druginfusion. A more marked and protracted elevation of plasma glucose was observed after infusion of gamma-glutamyl DA. By gamma-glutamyl DOPA had no effect on plasma glucose levels in spite of the high accumulation of DA in the pancreas after this prodrug. Of the various dopamine produrgs studied only gamma-glutamyl DOPA was not hyperglycemic in doses that are known to increase renal plasma flow in the rat. A simplified new procedure for the synthesis of gamma-glutamyl DA is described."}
{"id": "PMID:488164", "title": "Influence of prazosin on cardiac reflex function.", "content": "Prazosin administration significantly decreased arterial blood pressure without affecting heart rate in pentobarbital-anesthetized dogs. Wile pressor and reflex bradycardic responses to angiotensin II were not altered by prazosin, reflex tachycardia produced produced by histamine and acetylcholine were significantly attenuated by prazosin. The failure of prazosin administration to cause an increase in heart rate may be due to the ability of the agent to inhibit reflex tachycardic responses to depressor stimuli, the mechanism of whcih needs to be further investigated.", "contents": "Influence of prazosin on cardiac reflex function. Prazosin administration significantly decreased arterial blood pressure without affecting heart rate in pentobarbital-anesthetized dogs. Wile pressor and reflex bradycardic responses to angiotensin II were not altered by prazosin, reflex tachycardia produced produced by histamine and acetylcholine were significantly attenuated by prazosin. The failure of prazosin administration to cause an increase in heart rate may be due to the ability of the agent to inhibit reflex tachycardic responses to depressor stimuli, the mechanism of whcih needs to be further investigated."}
{"id": "PMID:488168", "title": "The actions of 5-hydroxytryptamine on isolated skeletal muscle preparations.", "content": "The effects of 5-hydroxytryptamine (5HT) on the mouse phrenic nerve--hemidiaphragm and frog sartorius muscle preparations were studied using twitch tension and intracellular recording methods. Between 1--7.5 mM 5HT transiently augmented the twitch response of the diaphragm to indirect, but not direct, stimulation. Above 5 mM 5HT the augmentation was followed by a concentration-dependent twitch blockade. The blockade was more apparent in anticholinesterase-treated preparations, and was seen to a lesser extent in directly stimulated muscles. Intracellular recording from diaphragm muscles revealed that 5HT depressed the amplitudes of both miniature endplate potentials (m.e.p.p.s.) and endplate potentials (e.p.p.s.), this effect being more pronounced in the presence of neostigmine. 5HT also greatly prolonged the duration of the e.p.p.s. In the sartorius muscles, 1--5 mM 5HT slowed and depressed action potentials. It is suggested that 5HT facilitates transmission by an anticholinesterase action and inhibits by blockade of receptor ion channels.", "contents": "The actions of 5-hydroxytryptamine on isolated skeletal muscle preparations. The effects of 5-hydroxytryptamine (5HT) on the mouse phrenic nerve--hemidiaphragm and frog sartorius muscle preparations were studied using twitch tension and intracellular recording methods. Between 1--7.5 mM 5HT transiently augmented the twitch response of the diaphragm to indirect, but not direct, stimulation. Above 5 mM 5HT the augmentation was followed by a concentration-dependent twitch blockade. The blockade was more apparent in anticholinesterase-treated preparations, and was seen to a lesser extent in directly stimulated muscles. Intracellular recording from diaphragm muscles revealed that 5HT depressed the amplitudes of both miniature endplate potentials (m.e.p.p.s.) and endplate potentials (e.p.p.s.), this effect being more pronounced in the presence of neostigmine. 5HT also greatly prolonged the duration of the e.p.p.s. In the sartorius muscles, 1--5 mM 5HT slowed and depressed action potentials. It is suggested that 5HT facilitates transmission by an anticholinesterase action and inhibits by blockade of receptor ion channels."}
{"id": "PMID:488169", "title": "Trazodone and imipramine: comparative effects on canine cardiac conduction.", "content": "Lead II EKG and His bundle electrograms were recorded in closed-chest pentobarbital-anesthetized dogs. Trazodone, a structurally distinct, new antidepressant agent, had no effect on His--Purkinje (HV interval) or intraventricular (HS interval) conduction following intravenous injection at graded doses between 1 and 30 mg/kg. In contrast, the tricyclic psychotropic agent imipramine (0.5--5 mg/kg) significantly slowed impulse conduction as indicated by increases in both the HV and HS intervals. Imipramine (5 mg/kg) also slowed impulse transmission across the atrium (PA interval). These responses to imipramine were associated with a concurrent prolongation of both the PR interval and QRS duration. In the anesthetized dog, trazodone administration promoted no evidence of heart block or sign of rhythm disturbances other than slowing in normal sinus rhythm.", "contents": "Trazodone and imipramine: comparative effects on canine cardiac conduction. Lead II EKG and His bundle electrograms were recorded in closed-chest pentobarbital-anesthetized dogs. Trazodone, a structurally distinct, new antidepressant agent, had no effect on His--Purkinje (HV interval) or intraventricular (HS interval) conduction following intravenous injection at graded doses between 1 and 30 mg/kg. In contrast, the tricyclic psychotropic agent imipramine (0.5--5 mg/kg) significantly slowed impulse conduction as indicated by increases in both the HV and HS intervals. Imipramine (5 mg/kg) also slowed impulse transmission across the atrium (PA interval). These responses to imipramine were associated with a concurrent prolongation of both the PR interval and QRS duration. In the anesthetized dog, trazodone administration promoted no evidence of heart block or sign of rhythm disturbances other than slowing in normal sinus rhythm."}
{"id": "PMID:488170", "title": "Differential binding of antidepressants to noradrenaline and serotonin transport sites in central nerve endings.", "content": "Imipramine and mianserin are equipotent inhibitors of noradrenaline (NA) uptake in synaptosomes. However, after in vivo administration, NA uptake was inhibited only in synaptosomes from imipramine-treated rats, suggesting that imipramine, or its metabolite desipramine, binds to the NA carrier in a manner outlasting the preparation of synaptosomes, whereas mianserin is washed away. To evaluate binding to the NA carrier, synaptosomes prelabeled with 3H-NA were pretreated with an antidepressant and the release of 3H-NA was then stimulated with unlabeled NA. Any reduction of release was taken as an indication of binding. Pretreatment with desipramine, but not with imipramine or mianserin, reduced 3H-NA release suggesting that desipramine is responsible for NA uptake inhibition in synaptosomes from imipramine-treated rats. Transformation of tertiary into secondary amines seems to be crucial for binding to the NA carrier, as confirmed by the stronger binding of nortriptyline and chlordesipramine compared to amitryptiline and chlorimipramine, respectively. In contrast, tertiary amines bound more strongly than secondary amines to the serotonin carrier. Adult and 8-day old synaptosomes showed similar binding properties towards imipramine and desipraine.", "contents": "Differential binding of antidepressants to noradrenaline and serotonin transport sites in central nerve endings. Imipramine and mianserin are equipotent inhibitors of noradrenaline (NA) uptake in synaptosomes. However, after in vivo administration, NA uptake was inhibited only in synaptosomes from imipramine-treated rats, suggesting that imipramine, or its metabolite desipramine, binds to the NA carrier in a manner outlasting the preparation of synaptosomes, whereas mianserin is washed away. To evaluate binding to the NA carrier, synaptosomes prelabeled with 3H-NA were pretreated with an antidepressant and the release of 3H-NA was then stimulated with unlabeled NA. Any reduction of release was taken as an indication of binding. Pretreatment with desipramine, but not with imipramine or mianserin, reduced 3H-NA release suggesting that desipramine is responsible for NA uptake inhibition in synaptosomes from imipramine-treated rats. Transformation of tertiary into secondary amines seems to be crucial for binding to the NA carrier, as confirmed by the stronger binding of nortriptyline and chlordesipramine compared to amitryptiline and chlorimipramine, respectively. In contrast, tertiary amines bound more strongly than secondary amines to the serotonin carrier. Adult and 8-day old synaptosomes showed similar binding properties towards imipramine and desipraine."}
{"id": "PMID:488171", "title": "Lack of influence of histamine antagonists on digitalis cardiotoxicity.", "content": "Experiments were carried out in order to study the interaction between cardioactive glycosides and histamine or histamine H1- AND/OR H2-receptor antagonists in anaesthetized cats or guinea pigs. The toxicity of ouabain or digoxin was tested by infusing the glycosides until idioventricular rhythm or death occurred. Histamine increased the toxicity of ouabain, but the histamine H1- and/or H2-receptor antagonists (metiamide and mepyramine)did not influence the toxicity of digitalis. In cats the increase in pulmonary arterial blood pressure caused by digoxin was not affectedby histamine H1- and/or H2-receptor antagonism in spite of the fact that the increase of pulmonary blood pressure caused by histamine could be abolished by H1-receptor antagonists. Our results indicate that histamine is not involved in the toxic effect of cardiac glycosides.", "contents": "Lack of influence of histamine antagonists on digitalis cardiotoxicity. Experiments were carried out in order to study the interaction between cardioactive glycosides and histamine or histamine H1- AND/OR H2-receptor antagonists in anaesthetized cats or guinea pigs. The toxicity of ouabain or digoxin was tested by infusing the glycosides until idioventricular rhythm or death occurred. Histamine increased the toxicity of ouabain, but the histamine H1- and/or H2-receptor antagonists (metiamide and mepyramine)did not influence the toxicity of digitalis. In cats the increase in pulmonary arterial blood pressure caused by digoxin was not affectedby histamine H1- and/or H2-receptor antagonism in spite of the fact that the increase of pulmonary blood pressure caused by histamine could be abolished by H1-receptor antagonists. Our results indicate that histamine is not involved in the toxic effect of cardiac glycosides."}
{"id": "PMID:488172", "title": "Serotonin release is modulated by presynaptic autoreceptors.", "content": "The existence of presynaptic autoreceptors controlling the release of 5-hydroxytryptamine (5HT) from serotonergic nerve endings was investigated utilizing superfused hypothalamic synaptosomes. Extracellular 5HT reduced the high K+-induced release of previously accumulated 3H-5HT. The central 5HT receptor blocker methiothepin counteracted the inhibitory effect of 5HT. Other 5HT antagonists (cyproheptadine, methysergide and mianserin) were inactive and may therefore act preferentially at the postsynaptic receptors.", "contents": "Serotonin release is modulated by presynaptic autoreceptors. The existence of presynaptic autoreceptors controlling the release of 5-hydroxytryptamine (5HT) from serotonergic nerve endings was investigated utilizing superfused hypothalamic synaptosomes. Extracellular 5HT reduced the high K+-induced release of previously accumulated 3H-5HT. The central 5HT receptor blocker methiothepin counteracted the inhibitory effect of 5HT. Other 5HT antagonists (cyproheptadine, methysergide and mianserin) were inactive and may therefore act preferentially at the postsynaptic receptors."}
{"id": "PMID:488187", "title": "Assay for thrombopoietin: a comparison of time of isotope incorporation into platelets and the effects of different strains and sexes of mice.", "content": "Several workers have used mice for thrombocytopoiesis-stimulating factor (TSF) assays, but the methods have differed. In quest of the optimum TSF assay conditions, we investigated the effects of interval between isotope injection and measurement of 35S incorporation into platelets, different mouse strains and sexes of mice. The 35S incorporation into platelets of mice increased with increase of the interval after isotope injection. However, the greatest difference in radioactivity between control and TSF-injected mice occurred at 16-40 h for normal mice and 24 h for rebound-thrombocytotic mice. When C3H, BALB/c or B6D2F1 mice were injected with platelet specific antisera, similar degrees of thrombocytopenia and rebound-thrombocytosis occurred. After injection of a standard dose of TSF, C3H and B6D2F1 mice showed greater isotopic incorporation levels, compared to suitable controls, than did BALB/c mice. Female and male mice exhibited essentially the same response to a standard dose of TSF. The data show that the mouse strain and isotopic incorporation time influence the sensitivity of the TSF assay but sex of test animals does not.", "contents": "Assay for thrombopoietin: a comparison of time of isotope incorporation into platelets and the effects of different strains and sexes of mice. Several workers have used mice for thrombocytopoiesis-stimulating factor (TSF) assays, but the methods have differed. In quest of the optimum TSF assay conditions, we investigated the effects of interval between isotope injection and measurement of 35S incorporation into platelets, different mouse strains and sexes of mice. The 35S incorporation into platelets of mice increased with increase of the interval after isotope injection. However, the greatest difference in radioactivity between control and TSF-injected mice occurred at 16-40 h for normal mice and 24 h for rebound-thrombocytotic mice. When C3H, BALB/c or B6D2F1 mice were injected with platelet specific antisera, similar degrees of thrombocytopenia and rebound-thrombocytosis occurred. After injection of a standard dose of TSF, C3H and B6D2F1 mice showed greater isotopic incorporation levels, compared to suitable controls, than did BALB/c mice. Female and male mice exhibited essentially the same response to a standard dose of TSF. The data show that the mouse strain and isotopic incorporation time influence the sensitivity of the TSF assay but sex of test animals does not."}
{"id": "PMID:488188", "title": "Growth patterns, cell composition, and mitotic activity of granulocytic aggregates developing in methyl cellulose from human normal marrow cells.", "content": "Marrow cells from five normal healthy subjects were cultured in methyl cellulose with and without leucocyte conditioned medium. Cultures were examined daily from day 3 to day 14 for the number of granulocytic aggregates and for their cell composition and mitotic activity. Aggregates of different size (small and large clusters, small and large colonies) displayed four distinct and characteristic patterns of growth. Unidentifiable blast cells were very rare throughout culture. The average proportion of recognizable granulocyte precursors (promyelocytes and myelocytes) (PMC + MC) declined during culture from 60% to 34%. There was a negative correlation between the size of the aggregates and their PMC + MC content, but on any day of culture some aggregates consisted mainly or uniquely of PMC + MC, while some others consisted mainly or uniquely of non-proliferating granulocytes. The mitotic index of PMC + MC was much higher than the one previously determined in vivo, and was significantly higher in large colonies than in other aggregates. These results are consistent with the recent demonstration of different CFU-C (sub)populations. It is suggested that differentiation of the aggregates according to their size and kinetics, coupled with cytological examination, can provide a more refined assessment of the assay, which could be useful for further comparison of normal, abnormal, and leukaemic granulocytopoiesis.", "contents": "Growth patterns, cell composition, and mitotic activity of granulocytic aggregates developing in methyl cellulose from human normal marrow cells. Marrow cells from five normal healthy subjects were cultured in methyl cellulose with and without leucocyte conditioned medium. Cultures were examined daily from day 3 to day 14 for the number of granulocytic aggregates and for their cell composition and mitotic activity. Aggregates of different size (small and large clusters, small and large colonies) displayed four distinct and characteristic patterns of growth. Unidentifiable blast cells were very rare throughout culture. The average proportion of recognizable granulocyte precursors (promyelocytes and myelocytes) (PMC + MC) declined during culture from 60% to 34%. There was a negative correlation between the size of the aggregates and their PMC + MC content, but on any day of culture some aggregates consisted mainly or uniquely of PMC + MC, while some others consisted mainly or uniquely of non-proliferating granulocytes. The mitotic index of PMC + MC was much higher than the one previously determined in vivo, and was significantly higher in large colonies than in other aggregates. These results are consistent with the recent demonstration of different CFU-C (sub)populations. It is suggested that differentiation of the aggregates according to their size and kinetics, coupled with cytological examination, can provide a more refined assessment of the assay, which could be useful for further comparison of normal, abnormal, and leukaemic granulocytopoiesis."}
{"id": "PMID:488189", "title": "Classification of animal lymphomas: the implications of applying Rappaport's classification for human lymphomas to experimental tumors.", "content": "One hundred and seventy animal lymphomas (species ranging from molluses to monkeys) were reclassified histologically according to the modified Rappaport classification for human lymphomas. The results were correlated with the etiology of the lymphomas, their clinical course, and in selected cases with their immunological type. The study stresses the value of such a procedure for comparative reasons, allowing a more adequate selection of animal models for human lymphomas.", "contents": "Classification of animal lymphomas: the implications of applying Rappaport's classification for human lymphomas to experimental tumors. One hundred and seventy animal lymphomas (species ranging from molluses to monkeys) were reclassified histologically according to the modified Rappaport classification for human lymphomas. The results were correlated with the etiology of the lymphomas, their clinical course, and in selected cases with their immunological type. The study stresses the value of such a procedure for comparative reasons, allowing a more adequate selection of animal models for human lymphomas."}
{"id": "PMID:488190", "title": "Trochanter bone marrow: a source of normal human colony forming cells.", "content": "In vitro studies of human hemopoiesis are often limited by the availability of normal bone marrow. We have overcome this difficulty by taking advantage of the bone marrow fragments removed during total hip replacement. We report here a comparative study of the colony forming capacity of trochanter and sternal or iliac crest marrow from five hematologically normal donors. Our data indicate that trochanter marrow is a reliable source of normal in vitro granulocyte/macrophage colony forming cells.", "contents": "Trochanter bone marrow: a source of normal human colony forming cells. In vitro studies of human hemopoiesis are often limited by the availability of normal bone marrow. We have overcome this difficulty by taking advantage of the bone marrow fragments removed during total hip replacement. We report here a comparative study of the colony forming capacity of trochanter and sternal or iliac crest marrow from five hematologically normal donors. Our data indicate that trochanter marrow is a reliable source of normal in vitro granulocyte/macrophage colony forming cells."}
{"id": "PMID:488191", "title": "Hydroxyurea suicide study of the kinetic heterogeneity of colony forming cells in human bone marrow.", "content": "The kinetics of granulomonocyte colony forming cells have been studied in unfractionated normal human bone marrow by hydroxyurea suicide and in cells separated by velocity sedimentation. Sequential studies revealed two subpopulations of colony forming cells, having different sizes and different multiplication potentialities. There are large cells with a high suicide rate which develop small granulocyte and monocyte colonies during the first week of culture in semi-solid agar. Smaller cells develop larger colonies of granulocytes, monocytes and eosinophils between 2 and 3 weeks of culture. Only granulocyte progenitors have a substantial suicide rate in this small cell population. This population is also less responsive to stimulation than is the large cell class, which is a more highly differentiated progeny. The role of these different kinetics of colony forming cells is discussed in the context of the heterogeneity of the in vitro differentiation of neutrophil, monocyte and eosinophil lines.", "contents": "Hydroxyurea suicide study of the kinetic heterogeneity of colony forming cells in human bone marrow. The kinetics of granulomonocyte colony forming cells have been studied in unfractionated normal human bone marrow by hydroxyurea suicide and in cells separated by velocity sedimentation. Sequential studies revealed two subpopulations of colony forming cells, having different sizes and different multiplication potentialities. There are large cells with a high suicide rate which develop small granulocyte and monocyte colonies during the first week of culture in semi-solid agar. Smaller cells develop larger colonies of granulocytes, monocytes and eosinophils between 2 and 3 weeks of culture. Only granulocyte progenitors have a substantial suicide rate in this small cell population. This population is also less responsive to stimulation than is the large cell class, which is a more highly differentiated progeny. The role of these different kinetics of colony forming cells is discussed in the context of the heterogeneity of the in vitro differentiation of neutrophil, monocyte and eosinophil lines."}
{"id": "PMID:488192", "title": "An improved plasma culture system for the production of megakaryocyte colonies in vitro.", "content": "A modified culture system has been developed to grow and quantitate megakaryocyte colonies from mouse bone marrow more efficiently than described in other reports. Using this method, it was shown that 30% of CFU-M in normal marrow cells and 70 to 90% of CFU-M in regenerating marrow cells were killed by high specific activity 3H-TdR in vitro. These results indicate that CFU-M are proliferating even in normal adult hemopoietic tissue, but that the proportion of cells that are proliferating is greater in regenerating marrow than in normal intact mice.", "contents": "An improved plasma culture system for the production of megakaryocyte colonies in vitro. A modified culture system has been developed to grow and quantitate megakaryocyte colonies from mouse bone marrow more efficiently than described in other reports. Using this method, it was shown that 30% of CFU-M in normal marrow cells and 70 to 90% of CFU-M in regenerating marrow cells were killed by high specific activity 3H-TdR in vitro. These results indicate that CFU-M are proliferating even in normal adult hemopoietic tissue, but that the proportion of cells that are proliferating is greater in regenerating marrow than in normal intact mice."}
{"id": "PMID:488193", "title": "Antiserum to mouse hematopoietic pluripotent stem cells (CFU-S): further investigation of its functional properties.", "content": "Treatment of mouse hematopoietic cells with heterologous antiserum raised against mouse brain markedly reduces the capacity of pluripotent stem cells (CFU-S) to form surface spleen colonies in lethally irradiated mice. To exclude the possibility that such treatment interferes only with the capacity of CFU-S to form surface spleen colonies, we evaluated the capacity of CFU-S which were treated with rabbit anti-mouse brain serum (RAMBS) to restore hematopoiesis and rescue lethally irradiated mice, and to form microscopic spleen colonies. Marrow cells were treated with RAMBS or control rabbit serum. Fifty thousand treated nucleated cells were injected i.v. into lethally irradiated mice, and hematopoietic reconstitution was studied between days 8 and 15; separate groups of mice were observed for survival. We found that treatment with RAMBS impairs the capacity of marrow cells to repopulate the marrow and spleen with CFU-S, to restore blood RBC, to effect an overshoot in spleen weight, and to prolong survival; in addition it reduces the number of microscopic spleen colonies to the same extent that it reduces the number of macroscopic colonies. Hence, RAMBS appears to effect a general inactivation of CFU-S and should prove to be a useful tool in further investigations of mouse hematopoiesis.", "contents": "Antiserum to mouse hematopoietic pluripotent stem cells (CFU-S): further investigation of its functional properties. Treatment of mouse hematopoietic cells with heterologous antiserum raised against mouse brain markedly reduces the capacity of pluripotent stem cells (CFU-S) to form surface spleen colonies in lethally irradiated mice. To exclude the possibility that such treatment interferes only with the capacity of CFU-S to form surface spleen colonies, we evaluated the capacity of CFU-S which were treated with rabbit anti-mouse brain serum (RAMBS) to restore hematopoiesis and rescue lethally irradiated mice, and to form microscopic spleen colonies. Marrow cells were treated with RAMBS or control rabbit serum. Fifty thousand treated nucleated cells were injected i.v. into lethally irradiated mice, and hematopoietic reconstitution was studied between days 8 and 15; separate groups of mice were observed for survival. We found that treatment with RAMBS impairs the capacity of marrow cells to repopulate the marrow and spleen with CFU-S, to restore blood RBC, to effect an overshoot in spleen weight, and to prolong survival; in addition it reduces the number of microscopic spleen colonies to the same extent that it reduces the number of macroscopic colonies. Hence, RAMBS appears to effect a general inactivation of CFU-S and should prove to be a useful tool in further investigations of mouse hematopoiesis."}
{"id": "PMID:488194", "title": "Evidence for proliferation of erythroid progenitor cells in the absence of added erythropoietin.", "content": "The cellular mechanisms underlying the requirement of leukocyte conditioned medium for human erythroid colony formation has been studied. Using photographic methods it was found that erythroid progenitor cells were able to form small colonies in the absence of added erythropoietin (epo). The addition of epo at this time leads to further growth and hemoglobinization of cells within the colony. Leukocyte conditioned medium was found to be required for this early proliferative stage of erythroid colony formation.", "contents": "Evidence for proliferation of erythroid progenitor cells in the absence of added erythropoietin. The cellular mechanisms underlying the requirement of leukocyte conditioned medium for human erythroid colony formation has been studied. Using photographic methods it was found that erythroid progenitor cells were able to form small colonies in the absence of added erythropoietin (epo). The addition of epo at this time leads to further growth and hemoglobinization of cells within the colony. Leukocyte conditioned medium was found to be required for this early proliferative stage of erythroid colony formation."}
{"id": "PMID:488195", "title": "Effect of inflammatory serum on 14C-glucosamine incorporation into bone marrow granulocytes in vitro.", "content": "As a preliminary approach to developing a biochemical assay for detecting humoral regulators of granulocyte maturation in the normal and inflammatory states, studies were carried out on the effects of normal and inflammatory sera on the incorporation of 14C-glucosamine into the glycoproteins of bone marrow granulocytes in vitro. We observed that, relative to normal serum, inflammatory serum had a marked stimulatory effect on 14C-glucosamine incorporation into these glycoproteins. This property of inflammatory serum reached a maximum at about 8 h after the initiation of inflammation in vivo and preceded the maximum increase in the mitotic activity of granulocyte precursors in the marrow by 18 h. It was also found that normal serum contains both dialyzable and heat-sensitive nondialyzable factors that inhibit 14C-glucosamine incorporation into bone marrow granulocytes in vitro. Data are presented which indicate that the stimulatory effect of inflammatory serum is most likely due to a nondialyzable factor which is capable of blocking the effect of the inhibitors present in normal serum.", "contents": "Effect of inflammatory serum on 14C-glucosamine incorporation into bone marrow granulocytes in vitro. As a preliminary approach to developing a biochemical assay for detecting humoral regulators of granulocyte maturation in the normal and inflammatory states, studies were carried out on the effects of normal and inflammatory sera on the incorporation of 14C-glucosamine into the glycoproteins of bone marrow granulocytes in vitro. We observed that, relative to normal serum, inflammatory serum had a marked stimulatory effect on 14C-glucosamine incorporation into these glycoproteins. This property of inflammatory serum reached a maximum at about 8 h after the initiation of inflammation in vivo and preceded the maximum increase in the mitotic activity of granulocyte precursors in the marrow by 18 h. It was also found that normal serum contains both dialyzable and heat-sensitive nondialyzable factors that inhibit 14C-glucosamine incorporation into bone marrow granulocytes in vitro. Data are presented which indicate that the stimulatory effect of inflammatory serum is most likely due to a nondialyzable factor which is capable of blocking the effect of the inhibitors present in normal serum."}
{"id": "PMID:488197", "title": "The dorsal spino-olivocerebellar system in the cat. II. Somatotopical organization.", "content": "The somatotopical organization of the projection through the dorsal spino-olivocerebellar path (DF-SOCP) to the c3 zone in the cerebellar anterior lobe was studied by recording climbing fiber field potentials in the cerebellar cortex evoked on stimulation of peripheral nerves. The c3 zone was shown to contain a detailed and systematic representation of the ipsilateral body half with the follwing characteristics: 1. Single nerves project to one or two sagittal strips of cortex which extend across several folia. The width of the strips varies between 0.2 and 1 mm and the length may be as long as 15-20 mm along the unfolded cortex. 2. The strips activated from different nerves occur in an order which usually follows the segmental innervation, the hindlimb being represented rostrally and the forelimb caudally in the zone. 3. The double representation of some nerves makes it possible to distinguish one medial and one lateral part of the c3 zone with the projection areas organized approximately as mirror images. Additional observations on other zones activated by the DF-SOCP system (Ekerot and Larson, 1979a) indicate that the c1 zone, has a somatotopical organization which is reminiscent of that of the c3 zone, whereas the x and d1 zones have different topographical organizations.", "contents": "The dorsal spino-olivocerebellar system in the cat. II. Somatotopical organization. The somatotopical organization of the projection through the dorsal spino-olivocerebellar path (DF-SOCP) to the c3 zone in the cerebellar anterior lobe was studied by recording climbing fiber field potentials in the cerebellar cortex evoked on stimulation of peripheral nerves. The c3 zone was shown to contain a detailed and systematic representation of the ipsilateral body half with the follwing characteristics: 1. Single nerves project to one or two sagittal strips of cortex which extend across several folia. The width of the strips varies between 0.2 and 1 mm and the length may be as long as 15-20 mm along the unfolded cortex. 2. The strips activated from different nerves occur in an order which usually follows the segmental innervation, the hindlimb being represented rostrally and the forelimb caudally in the zone. 3. The double representation of some nerves makes it possible to distinguish one medial and one lateral part of the c3 zone with the projection areas organized approximately as mirror images. Additional observations on other zones activated by the DF-SOCP system (Ekerot and Larson, 1979a) indicate that the c1 zone, has a somatotopical organization which is reminiscent of that of the c3 zone, whereas the x and d1 zones have different topographical organizations."}
{"id": "PMID:488198", "title": "An electrophysiological study of thalamo-caudate neurones in the cat.", "content": "Thalamo-caudate projection neurones were identified in cats by antidromic activation from the caudate nucleus, under chloralose anaesthesia or in cerveau isol\u00e9 preparations. Units in nuclei centralis lateralis, medialis dorsalis, and centrum medianum-parafascicularis responded antidromically to one or more electrodes of a caudate nucleus array at latencies ranging from 0.4 to 16 ms. The responses did not appear to result from stimulus spread to the internal capsule. A separate population of medial thalamic units responded postsynaptically to caudate stimulation, at modal latency exceeding modal antidromic latency by 2 ms. Comparison of apparent conduction velocities for each thalamic nucleus indicated that the postsynaptic responses were activated by collaterals of thalamo-caudate neurones. A proportion of thalamo-caudate neurones responded to somatic stimulation; some of these were inhibited by caudate stimulation, and a few discharged on substantia nigra stimulation. These results demonstrate the possibility of somatic afferent and nigrofugal inputs to the caudate nucleus mediated by neurones of the medial thalamus.", "contents": "An electrophysiological study of thalamo-caudate neurones in the cat. Thalamo-caudate projection neurones were identified in cats by antidromic activation from the caudate nucleus, under chloralose anaesthesia or in cerveau isol\u00e9 preparations. Units in nuclei centralis lateralis, medialis dorsalis, and centrum medianum-parafascicularis responded antidromically to one or more electrodes of a caudate nucleus array at latencies ranging from 0.4 to 16 ms. The responses did not appear to result from stimulus spread to the internal capsule. A separate population of medial thalamic units responded postsynaptically to caudate stimulation, at modal latency exceeding modal antidromic latency by 2 ms. Comparison of apparent conduction velocities for each thalamic nucleus indicated that the postsynaptic responses were activated by collaterals of thalamo-caudate neurones. A proportion of thalamo-caudate neurones responded to somatic stimulation; some of these were inhibited by caudate stimulation, and a few discharged on substantia nigra stimulation. These results demonstrate the possibility of somatic afferent and nigrofugal inputs to the caudate nucleus mediated by neurones of the medial thalamus."}
{"id": "PMID:488199", "title": "Functional properties of pulvinar-lateral posterior neurons which receive input from the superior colliculus.", "content": "Focal electrical stimulation of the superior colliculus induced short latency responses and/or a long duration suppression of spontaneous activity in single cells isolated in the pulvinar and lateral posterior (LP) nuclei of the cat. Cells which responded with one or more spikes at short latencies were relatively few in number, and these tended to be encountered in or near the principal tectorecipient zone of LP. In contrast, a suppression response was observed in many more cells which were widely distributed throughout the pulvinar-LP complex. Stimulation of the superficial layers of the colliculus was more effective in driving or suppressing pulvinar-LP cells than that of intermediate and deep layers. There was also an indication of a coarse topography in this projection. On the basis of their sensory response properties the cells responsive to tectal stimulation, at short latency or with a suppression, could not be differentiated from the overall sample of pulvinar-LP neurons.", "contents": "Functional properties of pulvinar-lateral posterior neurons which receive input from the superior colliculus. Focal electrical stimulation of the superior colliculus induced short latency responses and/or a long duration suppression of spontaneous activity in single cells isolated in the pulvinar and lateral posterior (LP) nuclei of the cat. Cells which responded with one or more spikes at short latencies were relatively few in number, and these tended to be encountered in or near the principal tectorecipient zone of LP. In contrast, a suppression response was observed in many more cells which were widely distributed throughout the pulvinar-LP complex. Stimulation of the superficial layers of the colliculus was more effective in driving or suppressing pulvinar-LP cells than that of intermediate and deep layers. There was also an indication of a coarse topography in this projection. On the basis of their sensory response properties the cells responsive to tectal stimulation, at short latency or with a suppression, could not be differentiated from the overall sample of pulvinar-LP neurons."}
{"id": "PMID:488200", "title": "A method for localized and longlasting microapplication of drugs into nervous tissue of freely moving animals.", "content": "A method is described for localized microapplication of drugs into nervous tissue of freely moving rats. Fairly constant release of material was observed over a period of more than three weeks. The amount of substance released was small because the rate of release was determined largely by diffusion rather than by mass movement of solution.", "contents": "A method for localized and longlasting microapplication of drugs into nervous tissue of freely moving animals. A method is described for localized microapplication of drugs into nervous tissue of freely moving rats. Fairly constant release of material was observed over a period of more than three weeks. The amount of substance released was small because the rate of release was determined largely by diffusion rather than by mass movement of solution."}
{"id": "PMID:488201", "title": "The elongation of fatty acids by microsomes and mitochondria from normal and pyridoxine-deficient rat brains.", "content": "The developmental profile of fatty acid elongation enzymes of rat brain microsomes and mitochondria and the consequences of pyridoxine deficiency have been investigated. The results suggest that a single enzyme system is responsible for elongation of the various fatty acids by mitochondria whereas the microsomal elongation system is diverse.", "contents": "The elongation of fatty acids by microsomes and mitochondria from normal and pyridoxine-deficient rat brains. The developmental profile of fatty acid elongation enzymes of rat brain microsomes and mitochondria and the consequences of pyridoxine deficiency have been investigated. The results suggest that a single enzyme system is responsible for elongation of the various fatty acids by mitochondria whereas the microsomal elongation system is diverse."}
{"id": "PMID:488203", "title": "Activity of ventrolateral thalamic neurons in relation to a simple reaction time task in the cat.", "content": "Unrestrained cats performed ballistic forelimb flexion movements triggered by an auditory stimulus (CS) on a simple reaction time (RT) paradigm. During the variable foreperiod the subject was required to hold down a lever and to release it on presentation of the CS. The RTs ranged from 200 to 300 ms. The activity of single neurons of the ventrolateral nucleus of the thalamus (VL) was recorded bilaterally. More than 40% of the 166 units recorded in the VL contralateral to the performing limb presented, after the CS, changes of activity with a latency less than 100 ms and were classified into three types: (1) Twenty-five units had a short latency transient increase of activity 10 to 30 ms after the CS, followed by a longer increase or decrease in activity. Short latency increase as well as subsequent increase of the firing rate were not correlated to the RTs. (2) Twenty-nine units showed a 40-60 ms latency increase of activity which lasted long enough to continue during the forelimb movement. These units displayed a correlation between the RTs and the mean firing rate measured in the 40-100 ms period  after the CS. The more the cells were activated, the shorter the RTs. (3) Fifteen units presented a reciprocal pattern of discharge with respect to the type (2) units. The firing rate decreased with latencies ranging from 20 to 90 ms after the CS. Only 14,5% of the 96 units recorded in the VL ipsilateral to the performing limb presented changes of activity starting in the 100 ms period following the CS. Background firing levels as well as phasic activity were rather low compared to those observed contralaterally. Sixteen units showed burst activity while the cat was performing but burst pattern was not time-related to the task. In an unconditioned animal, a very low level of activity and an absence of modulations were observed in both VLs.", "contents": "Activity of ventrolateral thalamic neurons in relation to a simple reaction time task in the cat. Unrestrained cats performed ballistic forelimb flexion movements triggered by an auditory stimulus (CS) on a simple reaction time (RT) paradigm. During the variable foreperiod the subject was required to hold down a lever and to release it on presentation of the CS. The RTs ranged from 200 to 300 ms. The activity of single neurons of the ventrolateral nucleus of the thalamus (VL) was recorded bilaterally. More than 40% of the 166 units recorded in the VL contralateral to the performing limb presented, after the CS, changes of activity with a latency less than 100 ms and were classified into three types: (1) Twenty-five units had a short latency transient increase of activity 10 to 30 ms after the CS, followed by a longer increase or decrease in activity. Short latency increase as well as subsequent increase of the firing rate were not correlated to the RTs. (2) Twenty-nine units showed a 40-60 ms latency increase of activity which lasted long enough to continue during the forelimb movement. These units displayed a correlation between the RTs and the mean firing rate measured in the 40-100 ms period  after the CS. The more the cells were activated, the shorter the RTs. (3) Fifteen units presented a reciprocal pattern of discharge with respect to the type (2) units. The firing rate decreased with latencies ranging from 20 to 90 ms after the CS. Only 14,5% of the 96 units recorded in the VL ipsilateral to the performing limb presented changes of activity starting in the 100 ms period following the CS. Background firing levels as well as phasic activity were rather low compared to those observed contralaterally. Sixteen units showed burst activity while the cat was performing but burst pattern was not time-related to the task. In an unconditioned animal, a very low level of activity and an absence of modulations were observed in both VLs."}
{"id": "PMID:488204", "title": "Effects of early visual and complex stimulation on learning, brain biochemistry, and electrophysiology.", "content": "A complex stimulation regimen (visual, auditory, and somesthetic-kinesthetic with forced movements, 30 times for 30 min each within 14 days) increased significantly the amplitudes of visual cortical evoked potentials (EPs) in adult rats if applied during the second postnatal fortnight. The EP increase after stimulation during the first 14 days after birth was not significant. Visual stimulation alone was compared with complex stimulation (visual plus forced movements) during the 2nd postnatal fortnight. More specific local changes in the visual cortex were revealed in brain biochemistry (lower DNA concentration, more RNA and protein per cell) and cortical electrogenesis (enhanced visual EPs) after visual stimulation alone, whereas complex stimulation induced more diffuse changes and rather profoundly influenced higher nervous functions (viz., memory retrieval - improved 24-h). Involvement of both specific and nonspecific mechanisms in the aftereffects of early stimulation is indicated.", "contents": "Effects of early visual and complex stimulation on learning, brain biochemistry, and electrophysiology. A complex stimulation regimen (visual, auditory, and somesthetic-kinesthetic with forced movements, 30 times for 30 min each within 14 days) increased significantly the amplitudes of visual cortical evoked potentials (EPs) in adult rats if applied during the second postnatal fortnight. The EP increase after stimulation during the first 14 days after birth was not significant. Visual stimulation alone was compared with complex stimulation (visual plus forced movements) during the 2nd postnatal fortnight. More specific local changes in the visual cortex were revealed in brain biochemistry (lower DNA concentration, more RNA and protein per cell) and cortical electrogenesis (enhanced visual EPs) after visual stimulation alone, whereas complex stimulation induced more diffuse changes and rather profoundly influenced higher nervous functions (viz., memory retrieval - improved 24-h). Involvement of both specific and nonspecific mechanisms in the aftereffects of early stimulation is indicated."}
{"id": "PMID:488205", "title": "Pontine and non-pontine pathways mediating early mossy fiber responses from sensorimotor cortex to cerebellum in the cat.", "content": "Stimulation of the cat's sensorimotor cortex evokes a short-latency mossy fiber field potential (N2) in the pars intermedia of the cerebellum. The fraction of the response carried by each of the six cerebellar peduncles was determined by comparing the field potentials before and after electrolytic lesions of individual cerebellar peduncles. The response evoked from the contralateral cerebrum is 3.5 times as large as that evoked from ipsilateral cerebrum. The size of the N2 wave from contralateral cerebral cortex is reduced to about half of the original size after electrolytic lesion of either the brachium pontis (BP) or the restiform body (RB) ipsilateral to the recording site, while only a small reduction in the size of N2 is observed after lesions of contralateral BP and RB. This indicates that inputs responsible for the generation of the N2 wave from contralateral cerebral cortex enter the cerebellum primarily through ipsilateral BP and RB. In contrast, the N2 wave from ipsilateral cerebral cortex is reduced by approximately one quarter following lesions of any one of the four RB's and BP's. Fibers in the brachium conjunctivum do not contribute to these responses. Observation on the P2 field potential, representing postsynaptic firing of granule cells, suggest that the cerebro-ponto-cerebellar and cerebro-reticulo-cerebellar mossy fiber pathways converge onto granule cells. Furthermore, these two inputs must arrive within 3 ms of each other in order to activate the granule cells. In addition, it is shown that BP lesions reducing the mossy fiber input lead to an increase in the climbing fiber input.", "contents": "Pontine and non-pontine pathways mediating early mossy fiber responses from sensorimotor cortex to cerebellum in the cat. Stimulation of the cat's sensorimotor cortex evokes a short-latency mossy fiber field potential (N2) in the pars intermedia of the cerebellum. The fraction of the response carried by each of the six cerebellar peduncles was determined by comparing the field potentials before and after electrolytic lesions of individual cerebellar peduncles. The response evoked from the contralateral cerebrum is 3.5 times as large as that evoked from ipsilateral cerebrum. The size of the N2 wave from contralateral cerebral cortex is reduced to about half of the original size after electrolytic lesion of either the brachium pontis (BP) or the restiform body (RB) ipsilateral to the recording site, while only a small reduction in the size of N2 is observed after lesions of contralateral BP and RB. This indicates that inputs responsible for the generation of the N2 wave from contralateral cerebral cortex enter the cerebellum primarily through ipsilateral BP and RB. In contrast, the N2 wave from ipsilateral cerebral cortex is reduced by approximately one quarter following lesions of any one of the four RB's and BP's. Fibers in the brachium conjunctivum do not contribute to these responses. Observation on the P2 field potential, representing postsynaptic firing of granule cells, suggest that the cerebro-ponto-cerebellar and cerebro-reticulo-cerebellar mossy fiber pathways converge onto granule cells. Furthermore, these two inputs must arrive within 3 ms of each other in order to activate the granule cells. In addition, it is shown that BP lesions reducing the mossy fiber input lead to an increase in the climbing fiber input."}
{"id": "PMID:488206", "title": "Evoked potential evidence for differences in binocularity between striate and prestriate regions of human visual cortex.", "content": "For two components of the visual evoked potential elicited by the onset of a stationary pattern the degree of interocular transfer of the attenuation caused by prior exposure to a similar pattern was measured. The results show almost complete interocular transfer for component CII, thought to originate in prestriate cortex, but only partial transfer of CI, thought to be of striate cortical origin. This suggests that in man, as in monkey, monocularly driven neurones are more common in striate than in prestriate cortex.", "contents": "Evoked potential evidence for differences in binocularity between striate and prestriate regions of human visual cortex. For two components of the visual evoked potential elicited by the onset of a stationary pattern the degree of interocular transfer of the attenuation caused by prior exposure to a similar pattern was measured. The results show almost complete interocular transfer for component CII, thought to originate in prestriate cortex, but only partial transfer of CI, thought to be of striate cortical origin. This suggests that in man, as in monkey, monocularly driven neurones are more common in striate than in prestriate cortex."}
{"id": "PMID:488207", "title": "A supernormal period in central axons following single cell stimulation.", "content": "Intracellular recordings were obtained from caudate (Cd) nucleus efferent neurons in the cat. A supernormal period, as characterized by increased excitability and conduction velocity, was found for the Cd efferent fibers following either a conditioning antidromic action potential, or a single orthodromic action potential induced by direct current application through the recording microelectrode. These data indicate that the impulse activity of a single fiber can modulate excitability and conduction velocity independent of surround impulse activity.", "contents": "A supernormal period in central axons following single cell stimulation. Intracellular recordings were obtained from caudate (Cd) nucleus efferent neurons in the cat. A supernormal period, as characterized by increased excitability and conduction velocity, was found for the Cd efferent fibers following either a conditioning antidromic action potential, or a single orthodromic action potential induced by direct current application through the recording microelectrode. These data indicate that the impulse activity of a single fiber can modulate excitability and conduction velocity independent of surround impulse activity."}
{"id": "PMID:488209", "title": "Comparison of dynamic properties of canal-evoked vestibulospinal reflexes of the neck and forelimb in the decerebrate cat.", "content": "The responses of neck and forelimb muscles to sinusoidal polarization of the horizontal canal nerve were compared by recording from these muscles simultaneously. Contrary to results on the vestibulocollic reflex, the central phase lag in the vestibulo-forelimb reflex increases with increasing frequencies up to 3 Hz. This demonstrates a difference in the organization of vestibular-driven pathways to neck and forelimb muscles.", "contents": "Comparison of dynamic properties of canal-evoked vestibulospinal reflexes of the neck and forelimb in the decerebrate cat. The responses of neck and forelimb muscles to sinusoidal polarization of the horizontal canal nerve were compared by recording from these muscles simultaneously. Contrary to results on the vestibulocollic reflex, the central phase lag in the vestibulo-forelimb reflex increases with increasing frequencies up to 3 Hz. This demonstrates a difference in the organization of vestibular-driven pathways to neck and forelimb muscles."}
{"id": "PMID:488210", "title": "The parieto-rubro-olivary pathway in the cat.", "content": "Stimulation of the parietal association cortex as well as the frontal motor cortex elicited clearly extracellular unitary activities or field potentials in the ipsilateral inferior olive in the cat. The parietal-induced responses came out generally at a longer and more variable latency than the frontal-induced ones. This suggested the existence of an indirect pathway from the parietal association cortex to the inferior olive. The recording sites for the parietal-induced responses were located not only in the dorsal lamella but also in the ventral lamella of the principal olive and in the medial accessory olive. Such olivary sites were exclusively in the rostral half of the inferior olive, and these areas in the olive were considered to give projection fibres predominantly to the hemispherical parts of the cerebe-lar cortex (neocerebellum). Small neuronal cells were labelled with horseradish peroxidase (HRP) homolaterally in the midbrain tegmentum, after HRP was injected through recording glass microelectrodes into the inferior olive where only the parietal-induced responses were evidently recorded. These small cells were distributed in the rostral one-third of the red nucleus and/or around the adjacent midbrain reticular formation close to the lateral border of the red nucleus. In referring to recent anatomical and physiological data, such small neurones labelled with HRP could be identified as the parvocellular red nucleus neurones. The present results indicate the existence of the parieto-rubro-olivary pathway system in the cat and suggest, in association with our previous studies, that the parvocellular red nucleus neurones participate in control of highly co-ordinated posture and movement predominantly through the neocerebellum.", "contents": "The parieto-rubro-olivary pathway in the cat. Stimulation of the parietal association cortex as well as the frontal motor cortex elicited clearly extracellular unitary activities or field potentials in the ipsilateral inferior olive in the cat. The parietal-induced responses came out generally at a longer and more variable latency than the frontal-induced ones. This suggested the existence of an indirect pathway from the parietal association cortex to the inferior olive. The recording sites for the parietal-induced responses were located not only in the dorsal lamella but also in the ventral lamella of the principal olive and in the medial accessory olive. Such olivary sites were exclusively in the rostral half of the inferior olive, and these areas in the olive were considered to give projection fibres predominantly to the hemispherical parts of the cerebe-lar cortex (neocerebellum). Small neuronal cells were labelled with horseradish peroxidase (HRP) homolaterally in the midbrain tegmentum, after HRP was injected through recording glass microelectrodes into the inferior olive where only the parietal-induced responses were evidently recorded. These small cells were distributed in the rostral one-third of the red nucleus and/or around the adjacent midbrain reticular formation close to the lateral border of the red nucleus. In referring to recent anatomical and physiological data, such small neurones labelled with HRP could be identified as the parvocellular red nucleus neurones. The present results indicate the existence of the parieto-rubro-olivary pathway system in the cat and suggest, in association with our previous studies, that the parvocellular red nucleus neurones participate in control of highly co-ordinated posture and movement predominantly through the neocerebellum."}
{"id": "PMID:488211", "title": "Suprachiasmatic nucleus neurones: excitation and inhibition mediated by the direct retino-hypothalamic projection in female rats.", "content": "The suprachiasmatic nucleus (SCN) of female rats was surveyed with microelectrodes under urethane anaesthesia. In rats with bilateral transection of the optic tracts, repetitive three pulses of 100 Hz applied to the contralateral optic nerve excited 8 and inhibited 11 other of the 86 SCN units examined. Transection of the optic tract did not significantly influence frequency of occurrence of the SCN units that were excited or inhibited by stimulation of the optic nerve. Certain SCN units responded to both of contralateral and ipsilateral stimulations of the optic nerve, indicating that bilateral visual inputs converge on the same single SCN neurones. Oscillatory responses with a period of 100--200 msec were occasionally produced by stimulation of the optic nerve. Flash stimuli with relatively weak intensity, even insufficient for producing wavelets in electroretinograms, produced an excitation and inhibition in SCN units. The mean firing rates were significantly altered by either electrical or flash stimuli repeated 500 times at 0.97 Hz in those units which showed no transitory response. Some of the SCN neurones receiving visual inputs were identified to be the tuberoinfundibular neurone and some other SCN neurones were found to receive converging inputs both from the optic nerve and from the axon collaterals of tuberoinfundibular neurones.", "contents": "Suprachiasmatic nucleus neurones: excitation and inhibition mediated by the direct retino-hypothalamic projection in female rats. The suprachiasmatic nucleus (SCN) of female rats was surveyed with microelectrodes under urethane anaesthesia. In rats with bilateral transection of the optic tracts, repetitive three pulses of 100 Hz applied to the contralateral optic nerve excited 8 and inhibited 11 other of the 86 SCN units examined. Transection of the optic tract did not significantly influence frequency of occurrence of the SCN units that were excited or inhibited by stimulation of the optic nerve. Certain SCN units responded to both of contralateral and ipsilateral stimulations of the optic nerve, indicating that bilateral visual inputs converge on the same single SCN neurones. Oscillatory responses with a period of 100--200 msec were occasionally produced by stimulation of the optic nerve. Flash stimuli with relatively weak intensity, even insufficient for producing wavelets in electroretinograms, produced an excitation and inhibition in SCN units. The mean firing rates were significantly altered by either electrical or flash stimuli repeated 500 times at 0.97 Hz in those units which showed no transitory response. Some of the SCN neurones receiving visual inputs were identified to be the tuberoinfundibular neurone and some other SCN neurones were found to receive converging inputs both from the optic nerve and from the axon collaterals of tuberoinfundibular neurones."}
{"id": "PMID:488212", "title": "The fine structural organization of the locus coeruleus in the rat with reference to noradrenaline contents.", "content": "Combination of glyoxylic acid perfusion and postfixation in permanganate was used in an electron microscopic study of the locus coeruleus (LC) of the rat to give good preservation of fine structure and a reproducible demonstration of noradrenaline (NA) storage granules. Medium-sized LC cells (18 x 30 microns) contained a moderate number of small granular vesicles (SGV) and a few large granular vesicles (LGV), mainly near the Golgi apparatus. Dendritic branches were identified by their SGV content up to the tip. Dendrites were occasionally in close contact with each other or with the soma of LC cells, forming dendro-dendritic or dendro-somatic contacts. Numerous axon terminals containing many SGV and some LGV were observed in the neuropil, and they tended to contact dendrites and somata of LC cells or dendrites of unknown origin. These neuronal contacts were devoid of synaptic specializations except for an array of dense perpendicular lines between the juxtaposed membranes. Small oval cells (10 x 15 microns) devoid of SGV occurred frequently in the peripheral part of the nucleus, and they were occasionally in direct contact with LC cells.", "contents": "The fine structural organization of the locus coeruleus in the rat with reference to noradrenaline contents. Combination of glyoxylic acid perfusion and postfixation in permanganate was used in an electron microscopic study of the locus coeruleus (LC) of the rat to give good preservation of fine structure and a reproducible demonstration of noradrenaline (NA) storage granules. Medium-sized LC cells (18 x 30 microns) contained a moderate number of small granular vesicles (SGV) and a few large granular vesicles (LGV), mainly near the Golgi apparatus. Dendritic branches were identified by their SGV content up to the tip. Dendrites were occasionally in close contact with each other or with the soma of LC cells, forming dendro-dendritic or dendro-somatic contacts. Numerous axon terminals containing many SGV and some LGV were observed in the neuropil, and they tended to contact dendrites and somata of LC cells or dendrites of unknown origin. These neuronal contacts were devoid of synaptic specializations except for an array of dense perpendicular lines between the juxtaposed membranes. Small oval cells (10 x 15 microns) devoid of SGV occurred frequently in the peripheral part of the nucleus, and they were occasionally in direct contact with LC cells."}
{"id": "PMID:488213", "title": "Visual modulation of otolith-dependent units in cat vestibular nuclei.", "content": "Vestibular nucleus units responding to translational self-motion are shown to respond also to translational movement of a large visual field. These visual-vestibular interactions in otolith-dependent units are similar to those found in canal-related units and could provide the basis for linearvection.", "contents": "Visual modulation of otolith-dependent units in cat vestibular nuclei. Vestibular nucleus units responding to translational self-motion are shown to respond also to translational movement of a large visual field. These visual-vestibular interactions in otolith-dependent units are similar to those found in canal-related units and could provide the basis for linearvection."}
{"id": "PMID:488214", "title": "Functional deficits in the optokinetic system of albino rats.", "content": "Vestibular nuclear neurons of the horizontal canal system (Vn) of albino rats (Wistar) failed to respond to optokinetic stimulation. Similarly, optokinetic nystagmus (OKN) could not be elicited in these animals. Brown (DA--HAN) rats, however, consistently showed optokinetic responses of Vn and OKN to identical stimuli.", "contents": "Functional deficits in the optokinetic system of albino rats. Vestibular nuclear neurons of the horizontal canal system (Vn) of albino rats (Wistar) failed to respond to optokinetic stimulation. Similarly, optokinetic nystagmus (OKN) could not be elicited in these animals. Brown (DA--HAN) rats, however, consistently showed optokinetic responses of Vn and OKN to identical stimuli."}
{"id": "PMID:488215", "title": "Abnormal orientation selectivity in both eyes of strabismic amblyopes.", "content": "In normal observers preadaptation to a parallel grating increases the contrast threshold for a line whereas a perpendicular grating has no effect. Such orientation selectivity was not found in the amblyopic eye of two out of five squinters. Only a weak after-effect produced with a grating parallel to the line was obtained in the good eye of four of the amblyopes while all of them show an abnormal threshold reduction following adaptation to a perpendicular grating. This suggests a relationship between abnormal binocular interaction during visual development and the organization of orientational mechanisms but does not explain the loss of visual acuity in amblyopia.", "contents": "Abnormal orientation selectivity in both eyes of strabismic amblyopes. In normal observers preadaptation to a parallel grating increases the contrast threshold for a line whereas a perpendicular grating has no effect. Such orientation selectivity was not found in the amblyopic eye of two out of five squinters. Only a weak after-effect produced with a grating parallel to the line was obtained in the good eye of four of the amblyopes while all of them show an abnormal threshold reduction following adaptation to a perpendicular grating. This suggests a relationship between abnormal binocular interaction during visual development and the organization of orientational mechanisms but does not explain the loss of visual acuity in amblyopia."}
{"id": "PMID:488216", "title": "Impairments in limb actions after dorsal funiculi section in cats.", "content": "A study of the forces exerted by cats against the ground when jumping from a platform equipped with force transducers, was carried out. The study, complemented by film analysis of the jump, attempted to disclose some of the mechanisms underlying the decrease in efficiency and accuracy in releasing a moving piece of liver observed after dorsal funiculi section above C1 level. The results showed that after the lesion, peak force and height of jump were significantly reduced. However, angle of take-off, an index of force distribution in the three axes of rectangular coordinates remained unchanged. Intact cats consistently extend their forelimbs in a smooth and progressive way toward the liver in order to release it. The distance between the tips of the forelimbs remained more or less constant during the flight. Postoperatively, instead of the regular pattern described, the extension of the limbs was interrupted by fast marked flexion movements and the distance between their tips increased significantly during the flight. Peak extension of the forelimbs was also decreased after surgery. The results are interpreted in terms of the different disruption of the various components (proprio and exteroceptive) that make up fasciculus gracilis and cuneatus of the dorsal funiculi.", "contents": "Impairments in limb actions after dorsal funiculi section in cats. A study of the forces exerted by cats against the ground when jumping from a platform equipped with force transducers, was carried out. The study, complemented by film analysis of the jump, attempted to disclose some of the mechanisms underlying the decrease in efficiency and accuracy in releasing a moving piece of liver observed after dorsal funiculi section above C1 level. The results showed that after the lesion, peak force and height of jump were significantly reduced. However, angle of take-off, an index of force distribution in the three axes of rectangular coordinates remained unchanged. Intact cats consistently extend their forelimbs in a smooth and progressive way toward the liver in order to release it. The distance between the tips of the forelimbs remained more or less constant during the flight. Postoperatively, instead of the regular pattern described, the extension of the limbs was interrupted by fast marked flexion movements and the distance between their tips increased significantly during the flight. Peak extension of the forelimbs was also decreased after surgery. The results are interpreted in terms of the different disruption of the various components (proprio and exteroceptive) that make up fasciculus gracilis and cuneatus of the dorsal funiculi."}
{"id": "PMID:488217", "title": "The role of maturation and visual experience in the development of eye alignment in cats.", "content": "In the developing kitten, the alignment of the pupils changes from strongly divergent to almost parallel. The visual axes, however, seem to stay almost parallel throughout this period. The infeluence of early visual experience on this development is examined in the present study. The results suggest that the develpment of eye alignment is not controlled by visual experience, but depends on maturational processes, and that normal visual input serves only to halt these processes at the appropriate time.", "contents": "The role of maturation and visual experience in the development of eye alignment in cats. In the developing kitten, the alignment of the pupils changes from strongly divergent to almost parallel. The visual axes, however, seem to stay almost parallel throughout this period. The infeluence of early visual experience on this development is examined in the present study. The results suggest that the develpment of eye alignment is not controlled by visual experience, but depends on maturational processes, and that normal visual input serves only to halt these processes at the appropriate time."}
{"id": "PMID:488218", "title": "A visual stimulator for orbiting light spots and equal energy annuli with continuously variable parameters.", "content": "When a light spot moves at high angular velocity along a circular path on a screen, an annulus is preceived. This principle is used in a simple annulus generator consisting of a plane mirror that rotates about an axis making an angle alpha with the normal in the center point of the mirror. Angle alpha can be varied with a micrometer screw to change the diameter of the annular path (orbit) followed by the light spot that is projected on a screen via the rotating mirror. This results in variable diameter equal energy annuli. The diameter of the light spot can be changed to manipulate the width of the annulus. Finally, at low angular velocities an orbiting light spot is obtained. It is shown in the companion paper (Molenaar and van de Grind, 1979) that this slow orbiting of the light spot allows a precise study of the fine structure of receptive fields.", "contents": "A visual stimulator for orbiting light spots and equal energy annuli with continuously variable parameters. When a light spot moves at high angular velocity along a circular path on a screen, an annulus is preceived. This principle is used in a simple annulus generator consisting of a plane mirror that rotates about an axis making an angle alpha with the normal in the center point of the mirror. Angle alpha can be varied with a micrometer screw to change the diameter of the annular path (orbit) followed by the light spot that is projected on a screen via the rotating mirror. This results in variable diameter equal energy annuli. The diameter of the light spot can be changed to manipulate the width of the annulus. Finally, at low angular velocities an orbiting light spot is obtained. It is shown in the companion paper (Molenaar and van de Grind, 1979) that this slow orbiting of the light spot allows a precise study of the fine structure of receptive fields."}
{"id": "PMID:488219", "title": "Deficits in the visual evoked potentials of cats as a result of visual deprivation.", "content": "Visual evoked potentials in response to contrast reversal of grating patterns were used as a measure of visual function in normal and visually deprived cats. In cats which had been dark reared from birth (BD cats) there was a characteristic change in VEP waveform from normal, for both eyes and for all spatial frequencies of testing stimulus. In cats which had one eye sutured from the age of one week (MD cats), the VEP from the deprived eye was smaller for contrast reversal of coarse patterns. Kittens given only restricted periods of monocular exposure gave VEPs which resembled the pathological responses of the BD cats. However, the amplitudes of response were larger for the more experienced eye at higher spatial frequency. This work reinforces the idea that two factors govern cortical development: competition and experience. Lack of sufficient visual experience leads to severe intracortical pathology.", "contents": "Deficits in the visual evoked potentials of cats as a result of visual deprivation. Visual evoked potentials in response to contrast reversal of grating patterns were used as a measure of visual function in normal and visually deprived cats. In cats which had been dark reared from birth (BD cats) there was a characteristic change in VEP waveform from normal, for both eyes and for all spatial frequencies of testing stimulus. In cats which had one eye sutured from the age of one week (MD cats), the VEP from the deprived eye was smaller for contrast reversal of coarse patterns. Kittens given only restricted periods of monocular exposure gave VEPs which resembled the pathological responses of the BD cats. However, the amplitudes of response were larger for the more experienced eye at higher spatial frequency. This work reinforces the idea that two factors govern cortical development: competition and experience. Lack of sufficient visual experience leads to severe intracortical pathology."}
{"id": "PMID:488220", "title": "Mechanisms of the clasp-knife reflex studied in an animal model.", "content": "The mechanisms of the clasp-knife reflex were studied in the soleus muscle of an animal model, the decerebrate cat with a dorsal hemisection of the lower thoracic cord. The reflex is shown to be autogenetic, and to depend on muscle length in keeping with previous suggestions. However, the magnitude of the inhibition increases with increasing initial force, and the inhibition is mimicked by gentle manipulation of the muscle and tendon surface. Concurrent muscle afferent recordings showed that the electromyogram (emg) reduction was not a result of a decline in Ia afferent input and was not well related to secondary or tendon organ afferent discharge. It is now known that many group III and some group IV muscle afferents are also activated by muscle stretch and contraction, and we here report limited stretch sensitivity in four non-spindle group II afferents. Since these fiber groups each include afferents that produce inhibition of extensor motoneurons, it is proposed that the clasp-knife reflex may result from the activation of these slowly conducting afferent fibers.", "contents": "Mechanisms of the clasp-knife reflex studied in an animal model. The mechanisms of the clasp-knife reflex were studied in the soleus muscle of an animal model, the decerebrate cat with a dorsal hemisection of the lower thoracic cord. The reflex is shown to be autogenetic, and to depend on muscle length in keeping with previous suggestions. However, the magnitude of the inhibition increases with increasing initial force, and the inhibition is mimicked by gentle manipulation of the muscle and tendon surface. Concurrent muscle afferent recordings showed that the electromyogram (emg) reduction was not a result of a decline in Ia afferent input and was not well related to secondary or tendon organ afferent discharge. It is now known that many group III and some group IV muscle afferents are also activated by muscle stretch and contraction, and we here report limited stretch sensitivity in four non-spindle group II afferents. Since these fiber groups each include afferents that produce inhibition of extensor motoneurons, it is proposed that the clasp-knife reflex may result from the activation of these slowly conducting afferent fibers."}
{"id": "PMID:488253", "title": "Biomimetic synthesis of cannabispiran.", "content": "Total synthesis of cannabispiran (1) was accomplished by a biomimetic-type cyclization of the bibenzyl 2, using K3Fe(CN)6 or MoCl4.", "contents": "Biomimetic synthesis of cannabispiran. Total synthesis of cannabispiran (1) was accomplished by a biomimetic-type cyclization of the bibenzyl 2, using K3Fe(CN)6 or MoCl4."}
{"id": "PMID:488254", "title": "Amino acid composition and sequence of crinia-angiotensin, an angiotensin II-like endecapeptide from the skin of the Australian frog Crinia georgiana.", "content": "Methanol extracts of the skin of the Australian amphibian Crinia georgiana contain large amounts of crinia-angiotensin II, a new angiotensin II-like peptide. This differs sharply from the conventional octapeptide angiotensins II in having attached the tripeptide Ala-Pro-Gly- to the N-terminus, and having an Ile residue substituted for the Val residue at position 6 from the C-terminus. Small amounts of angiotensin-like peptides have been traced, by radioimmunoassay, in skin extracts of some other Crinia species.", "contents": "Amino acid composition and sequence of crinia-angiotensin, an angiotensin II-like endecapeptide from the skin of the Australian frog Crinia georgiana. Methanol extracts of the skin of the Australian amphibian Crinia georgiana contain large amounts of crinia-angiotensin II, a new angiotensin II-like peptide. This differs sharply from the conventional octapeptide angiotensins II in having attached the tripeptide Ala-Pro-Gly- to the N-terminus, and having an Ile residue substituted for the Val residue at position 6 from the C-terminus. Small amounts of angiotensin-like peptides have been traced, by radioimmunoassay, in skin extracts of some other Crinia species."}
{"id": "PMID:488255", "title": "Occurrence of Hyp3-bradykinin in methanol extracts of the skin of the South African leptodactylid frog Heleophryne purcelli.", "content": "Methanol extracts of the skin of the South African amphibians belonging to the genus Heleophryne (H. purcelli purcelli, H. purcelli depressa, H. purcelli orientalis, H. natalensis) contain large amounts (20--500 microgram/g fresh tissue) of Hyp3-bradykinin.", "contents": "Occurrence of Hyp3-bradykinin in methanol extracts of the skin of the South African leptodactylid frog Heleophryne purcelli. Methanol extracts of the skin of the South African amphibians belonging to the genus Heleophryne (H. purcelli purcelli, H. purcelli depressa, H. purcelli orientalis, H. natalensis) contain large amounts (20--500 microgram/g fresh tissue) of Hyp3-bradykinin."}
{"id": "PMID:488256", "title": "Effect of sodium chloride on the respiratory function of Staphylococcus aureus.", "content": "Sodium chloride at concentrations below 0.5 M, enhanced the respiratory activity (O2-consumption) of Staphylococcus aureus under endogenous and sugar-supported conditions, but did not overcome the inhibitory action of sodium azide. Several sugars, including the glucose analogue alpha-methylglucoside, and their metabolites enhanced bacterial O2-consumption, but acetylmethylcarbinol was ineffective.", "contents": "Effect of sodium chloride on the respiratory function of Staphylococcus aureus. Sodium chloride at concentrations below 0.5 M, enhanced the respiratory activity (O2-consumption) of Staphylococcus aureus under endogenous and sugar-supported conditions, but did not overcome the inhibitory action of sodium azide. Several sugars, including the glucose analogue alpha-methylglucoside, and their metabolites enhanced bacterial O2-consumption, but acetylmethylcarbinol was ineffective."}
{"id": "PMID:488257", "title": "The turnover of F-actin-bound ADP in vivo.", "content": "A procedure for estimating the rate of turnover of F-actin-bound ADP in vivo is described. A turnover rate of 0.88 h-1 was determined for mouse muscle F-actin. The validity of the method when used to estimate the turnover rate of F-actin per se is discussed in relation to the possible exchange of F-actin-bound ADP.", "contents": "The turnover of F-actin-bound ADP in vivo. A procedure for estimating the rate of turnover of F-actin-bound ADP in vivo is described. A turnover rate of 0.88 h-1 was determined for mouse muscle F-actin. The validity of the method when used to estimate the turnover rate of F-actin per se is discussed in relation to the possible exchange of F-actin-bound ADP."}
{"id": "PMID:488258", "title": "Evidence of soluble proteins binding adriamycin by affinity chromatography.", "content": "The presence of various tissues of soluble proteins binding adriamycin is evidenced by affinity chromatography.", "contents": "Evidence of soluble proteins binding adriamycin by affinity chromatography. The presence of various tissues of soluble proteins binding adriamycin is evidenced by affinity chromatography."}
{"id": "PMID:488259", "title": "The amino acid composition of histidine ammonialyase from Pseudomonas putida NCIB 10807.", "content": "The amino acid composition of histidine ammonia-lyase from Pseudomonas putida NCIB 10807 suggests that this enzyme may be different from the Pseudomonas testosteroni NCIB 10808 histidine ammonia-lyase, whose amino acid composition is known.", "contents": "The amino acid composition of histidine ammonialyase from Pseudomonas putida NCIB 10807. The amino acid composition of histidine ammonia-lyase from Pseudomonas putida NCIB 10807 suggests that this enzyme may be different from the Pseudomonas testosteroni NCIB 10808 histidine ammonia-lyase, whose amino acid composition is known."}
{"id": "PMID:488260", "title": "Chloroperoxidase-catalyzed oxidation of N-methyl-4-chloroaniline.", "content": "Chloroperoxidase catalyzed the H2O2 oxidative conversion of N-methyl-4-chloroaniline to 4-chloronitrosobenzene, 4-chloroaniline and a mixture of complex products.", "contents": "Chloroperoxidase-catalyzed oxidation of N-methyl-4-chloroaniline. Chloroperoxidase catalyzed the H2O2 oxidative conversion of N-methyl-4-chloroaniline to 4-chloronitrosobenzene, 4-chloroaniline and a mixture of complex products."}
{"id": "PMID:488261", "title": "The role of sialic acid in 5-HT binding to synaptic membranes.", "content": "The high affinity binding of [14C]5-HT to nerve ending membranes isolated from rat brain is not affected by neuraminidase treatment. The specificity of ligand receptor interaction was demonstrated by displacement studies with tryptamine derivatives, noradrenaline, and acetylcholine.", "contents": "The role of sialic acid in 5-HT binding to synaptic membranes. The high affinity binding of [14C]5-HT to nerve ending membranes isolated from rat brain is not affected by neuraminidase treatment. The specificity of ligand receptor interaction was demonstrated by displacement studies with tryptamine derivatives, noradrenaline, and acetylcholine."}
{"id": "PMID:488262", "title": "Bisalbumineamia in a bottlenosed dolphin (Tursiops truncatus).", "content": "Bisalbuminemia was found in a female bottlenosed dolphin (Turiops truncatus) on routine examination. There is no association with disease.", "contents": "Bisalbumineamia in a bottlenosed dolphin (Tursiops truncatus). Bisalbuminemia was found in a female bottlenosed dolphin (Turiops truncatus) on routine examination. There is no association with disease."}
{"id": "PMID:488263", "title": "Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm.", "content": "Electron microscopy of the partially heat denatured ribosomal DNA (rDNA) from sea urchin (Lytechinus variegatus) sperm has demonstrated that it consists of repeating units of 3.6 +/- 0.2 micron, corresponding to a mol.wt of 7.2 +/- 0.4 x 10(6). Based on differential denaturability, each repeat unit is divided into 2 regions. The larger region of 2.47 +/- 0.11 micron (mol.wt 4.9 +/- 0.22 x 10(6)) corresponds in length to the ribosomal precursor RNA of sea urchins and the smaller, GC-rich, subunit of 1.16 +/- 0.09 micrometer (mol.wt 2.3 +/- 0.18 x 10(6)) is presumed to contain non-transcribed spacer sequences.", "contents": "Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm. Electron microscopy of the partially heat denatured ribosomal DNA (rDNA) from sea urchin (Lytechinus variegatus) sperm has demonstrated that it consists of repeating units of 3.6 +/- 0.2 micron, corresponding to a mol.wt of 7.2 +/- 0.4 x 10(6). Based on differential denaturability, each repeat unit is divided into 2 regions. The larger region of 2.47 +/- 0.11 micron (mol.wt 4.9 +/- 0.22 x 10(6)) corresponds in length to the ribosomal precursor RNA of sea urchins and the smaller, GC-rich, subunit of 1.16 +/- 0.09 micrometer (mol.wt 2.3 +/- 0.18 x 10(6)) is presumed to contain non-transcribed spacer sequences."}
{"id": "PMID:488264", "title": "Heterogeneity of DNA methylation in murine L5178Y lymphoblasts.", "content": "Comparison of the extent of methylation in mouse DNA fragments rendered MgCl2 soluble after mild DNase II digestion of nuclei, with different reassociation rate and nucleoli-bound, revealed the existence of 3 regions of the genome particularly 5-methylcytosine-rich: the sequences considered to be related to the transcriptionally active chromatin with the highest content of this base and fast reassociating, as well as nucleolar DNA with somewhat lower proportion of the methylated cytosines.", "contents": "Heterogeneity of DNA methylation in murine L5178Y lymphoblasts. Comparison of the extent of methylation in mouse DNA fragments rendered MgCl2 soluble after mild DNase II digestion of nuclei, with different reassociation rate and nucleoli-bound, revealed the existence of 3 regions of the genome particularly 5-methylcytosine-rich: the sequences considered to be related to the transcriptionally active chromatin with the highest content of this base and fast reassociating, as well as nucleolar DNA with somewhat lower proportion of the methylated cytosines."}
{"id": "PMID:488265", "title": "Colchicine-induced resistance to antibiotic and amino-acid analogue in plant cell cultures.", "content": "Colchicine-resistant plant cell strains have been isolated from cell suspensions of carrot and sycamore. In the same way as colchicine-resistant animal cell strains, they show an increased resistance towards streptomycin and N-methylalanine. Cultivation under non selective conditions leads to a slow progressive loss of the resistance.", "contents": "Colchicine-induced resistance to antibiotic and amino-acid analogue in plant cell cultures. Colchicine-resistant plant cell strains have been isolated from cell suspensions of carrot and sycamore. In the same way as colchicine-resistant animal cell strains, they show an increased resistance towards streptomycin and N-methylalanine. Cultivation under non selective conditions leads to a slow progressive loss of the resistance."}
{"id": "PMID:488266", "title": "Hydrogen bonding by the sulphydryl group of glutathione.", "content": "The occurrence of hydrogen bond in the sulphydryl group of glutathione was investigated by means of Raman Spectroscopy. Evidence is obtained that SH group is free and H-bonding does not occur.", "contents": "Hydrogen bonding by the sulphydryl group of glutathione. The occurrence of hydrogen bond in the sulphydryl group of glutathione was investigated by means of Raman Spectroscopy. Evidence is obtained that SH group is free and H-bonding does not occur."}
{"id": "PMID:488267", "title": "GFA expression in aggregation cultures of rat C6 glioma.", "content": "Few C6 glioma cells synthesize the astroglia-specific GFA protein in monolayer culture. A uniform population of GFA-positive cells was obtained by aggregation C6 cells in suspension culture, as previously reported for C6 glioma maintained on sponge foam matrices. These results strongly suggest that cell-to-cell interactions promote GFA expression.", "contents": "GFA expression in aggregation cultures of rat C6 glioma. Few C6 glioma cells synthesize the astroglia-specific GFA protein in monolayer culture. A uniform population of GFA-positive cells was obtained by aggregation C6 cells in suspension culture, as previously reported for C6 glioma maintained on sponge foam matrices. These results strongly suggest that cell-to-cell interactions promote GFA expression."}
{"id": "PMID:488268", "title": "Comparison of the biological effects of anemia inducing and polycythemia inducing Friend virus complex.", "content": "The comparison of the biological effects of FVP and FVA showed that leukemogenesis appears to be delayed in FVA infected mice as compared to FVP infected animals after injection of comparable quantities of virus as measured in spleen focus forming units. In addition, no CFU-EI, characteristic for FVP induced leukemia, were found in leukemic spleen or bone marrow of FVA infected mice. Since it was possible to distinguish both viruses by their different host ranges, which are helper virus determined, it is suggested that the observed differences, especially the lack of CFU-EI in FVA infected mice, might be due to differences in the helper virus component of the FV complex.", "contents": "Comparison of the biological effects of anemia inducing and polycythemia inducing Friend virus complex. The comparison of the biological effects of FVP and FVA showed that leukemogenesis appears to be delayed in FVA infected mice as compared to FVP infected animals after injection of comparable quantities of virus as measured in spleen focus forming units. In addition, no CFU-EI, characteristic for FVP induced leukemia, were found in leukemic spleen or bone marrow of FVA infected mice. Since it was possible to distinguish both viruses by their different host ranges, which are helper virus determined, it is suggested that the observed differences, especially the lack of CFU-EI in FVA infected mice, might be due to differences in the helper virus component of the FV complex."}
{"id": "PMID:488269", "title": "[An ultrastructural study of the transformation of elementary bodies of Rickettsiella into initial bodies (author's transl)].", "content": "An intermediate stage between the elementary and the initial bodies of the Rickettsietta genus is defined as the beginning of an intracellular cycle. It is characterized by several structural changes in the dense elementary body: the cytoplasm becomes less electron-dense; thus, the nucleoid and the ribosomes are visible. The inner layer of the cell-wall becomes progressively clearer and the trilamellar structure of the inner and outer membranes appears distinctly. 'preinitial body' is proposed as name of this stage of development.", "contents": "[An ultrastructural study of the transformation of elementary bodies of Rickettsiella into initial bodies (author's transl)]. An intermediate stage between the elementary and the initial bodies of the Rickettsietta genus is defined as the beginning of an intracellular cycle. It is characterized by several structural changes in the dense elementary body: the cytoplasm becomes less electron-dense; thus, the nucleoid and the ribosomes are visible. The inner layer of the cell-wall becomes progressively clearer and the trilamellar structure of the inner and outer membranes appears distinctly. 'preinitial body' is proposed as name of this stage of development."}
{"id": "PMID:488270", "title": "Lithium and rubidium: effects on the rhythmic swimming movement of jellyfish (Aurelia aurita).", "content": "The effects of adding LiCl, RbCl, KCl or NaCl to sea water at concentrations up to 30 mmoles/1 on the frequency of contraction of jellyfish (Aurelia aurita) suggest that studies on phylogenetically low animals with relatively simple nervous systems may be of use to determine mechanisms of action of lithium and rudidium on movements.", "contents": "Lithium and rubidium: effects on the rhythmic swimming movement of jellyfish (Aurelia aurita). The effects of adding LiCl, RbCl, KCl or NaCl to sea water at concentrations up to 30 mmoles/1 on the frequency of contraction of jellyfish (Aurelia aurita) suggest that studies on phylogenetically low animals with relatively simple nervous systems may be of use to determine mechanisms of action of lithium and rudidium on movements."}
{"id": "PMID:488271", "title": "Localization of concansvalin-A binding sites in Tetrahymena by scanning electron microscopy.", "content": "Binding sites of concanavalin-A were detected in Tetrahymena on the body ciliature, while the cell membrane itself and the oral ciliature failed to bind the lectine.", "contents": "Localization of concansvalin-A binding sites in Tetrahymena by scanning electron microscopy. Binding sites of concanavalin-A were detected in Tetrahymena on the body ciliature, while the cell membrane itself and the oral ciliature failed to bind the lectine."}
{"id": "PMID:488272", "title": "Phosphatase activity in testis and prostate of rats treated with embelin and Vinca rosea extract.", "content": "Daily administration of Vinca rosea Linn. extract orally and embelin s.c. to male albino rats caused significant rise in levels of acid and alkaline phosphatases of testis and prostate indicating altered metabolic function.", "contents": "Phosphatase activity in testis and prostate of rats treated with embelin and Vinca rosea extract. Daily administration of Vinca rosea Linn. extract orally and embelin s.c. to male albino rats caused significant rise in levels of acid and alkaline phosphatases of testis and prostate indicating altered metabolic function."}
{"id": "PMID:488273", "title": "Water regulation in Barmer goat of the Rajasthan desert.", "content": "The different body fluid compartments in normally watered and 4-day water-deprived goats of Rajasthan desert, India, were measured in autumn. The goats maintained plasma volume and extracellular fluid volume, but lost gut and cell water considerably under the experimental conditions; indicating that the maintenance of the fluidity of the blood has priority over the body's other fluid requirements in this desert-adapted species during water deprivation.", "contents": "Water regulation in Barmer goat of the Rajasthan desert. The different body fluid compartments in normally watered and 4-day water-deprived goats of Rajasthan desert, India, were measured in autumn. The goats maintained plasma volume and extracellular fluid volume, but lost gut and cell water considerably under the experimental conditions; indicating that the maintenance of the fluidity of the blood has priority over the body's other fluid requirements in this desert-adapted species during water deprivation."}
{"id": "PMID:488274", "title": "Electrical response of bat retina to spectral stimulation: comparison of four microhiropteran species.", "content": "Electrical responses of the retinas of 4 species of microchiropteran bats stimulated by spectrally restricted light flashes were found to diverge systematically from the rhodopsin absorption spectrum. The divergence was progressively greater across the 4 species. The results appeared explainable by assuming a second photoreceptor class and photopigment which was present in progressively greater numbers in the retinas of Eptesicus fuscus, Desmodus rotundus, Artibeus jamaicensis and Carollia perspicillata.", "contents": "Electrical response of bat retina to spectral stimulation: comparison of four microhiropteran species. Electrical responses of the retinas of 4 species of microchiropteran bats stimulated by spectrally restricted light flashes were found to diverge systematically from the rhodopsin absorption spectrum. The divergence was progressively greater across the 4 species. The results appeared explainable by assuming a second photoreceptor class and photopigment which was present in progressively greater numbers in the retinas of Eptesicus fuscus, Desmodus rotundus, Artibeus jamaicensis and Carollia perspicillata."}
{"id": "PMID:488275", "title": "Effect of light adaptation on electrical responses of the retinas of four species of bats.", "content": "The levels of light adaptation at which the retinas of 4 species of microchiropteran bats became unable to generate electroretinograms were progressively ordered. The order correlated well with light preferences based on activity patterns of the 4 species. These results suggest that the ability of the retina to function in ambient light may govern some natural behaviors of these bats.", "contents": "Effect of light adaptation on electrical responses of the retinas of four species of bats. The levels of light adaptation at which the retinas of 4 species of microchiropteran bats became unable to generate electroretinograms were progressively ordered. The order correlated well with light preferences based on activity patterns of the 4 species. These results suggest that the ability of the retina to function in ambient light may govern some natural behaviors of these bats."}
{"id": "PMID:488276", "title": "Resting heartrate variability in man declines with age.", "content": "The heartrate variability under resting conditions of 14 normal male subjects, age range 22--63 years, declined with increasing age. Mean heartrate did not show age-dependant changes.", "contents": "Resting heartrate variability in man declines with age. The heartrate variability under resting conditions of 14 normal male subjects, age range 22--63 years, declined with increasing age. Mean heartrate did not show age-dependant changes."}
{"id": "PMID:488277", "title": "Interaction between human evoked electrospinograms elicited by segmental and descending volleys.", "content": "Interaction between the slow negative-positive waves of human evoked electrospinograms produced by descending and segmental volleys was tested under general anaesthesia. A partial occlusion was demonstrated in these slow waves.", "contents": "Interaction between human evoked electrospinograms elicited by segmental and descending volleys. Interaction between the slow negative-positive waves of human evoked electrospinograms produced by descending and segmental volleys was tested under general anaesthesia. A partial occlusion was demonstrated in these slow waves."}
{"id": "PMID:488278", "title": "Electrophysiology of mammalian hypothalamic and interpeduncular neurons in vitro.", "content": "Electrical activities of the hypothalamic and interpeduncular neurons were studied in vitro in brain slices prepared from the guinea-pig brain stem. Neurons preserved resting membrane potentials comparable to those of neurons in vivo, responded to stimulation of the afferent fibres, and retained stable spontaneous firings for more than several hours.", "contents": "Electrophysiology of mammalian hypothalamic and interpeduncular neurons in vitro. Electrical activities of the hypothalamic and interpeduncular neurons were studied in vitro in brain slices prepared from the guinea-pig brain stem. Neurons preserved resting membrane potentials comparable to those of neurons in vivo, responded to stimulation of the afferent fibres, and retained stable spontaneous firings for more than several hours."}
{"id": "PMID:488279", "title": "Receptor potential of rat taste cell to potassium benzoate.", "content": "Rat taste cells responded to relatively low concentrations of K-benzoate with a hyperpolarization and to the high concentrations with a depolarization. During both responses the membrane resistance of a taste cell decreased. Depolarization elicited by application of a combination of 0.25 M NaCl and 0.05 M K-benzoate was smaller than that by the NaCl alone, indicating a depressant action of K-benzoate.", "contents": "Receptor potential of rat taste cell to potassium benzoate. Rat taste cells responded to relatively low concentrations of K-benzoate with a hyperpolarization and to the high concentrations with a depolarization. During both responses the membrane resistance of a taste cell decreased. Depolarization elicited by application of a combination of 0.25 M NaCl and 0.05 M K-benzoate was smaller than that by the NaCl alone, indicating a depressant action of K-benzoate."}
{"id": "PMID:488280", "title": "The effects of dopamine on renin release in the isolated perfused rat kidney.", "content": "In the isolated and perfused kidney of the rat, the stimulant effect of dopamine on renin release is blocked by propranolol and not by haloperidol. This suggests that the release of renin induced by dopamine is due to the activation of beta-receptors.", "contents": "The effects of dopamine on renin release in the isolated perfused rat kidney. In the isolated and perfused kidney of the rat, the stimulant effect of dopamine on renin release is blocked by propranolol and not by haloperidol. This suggests that the release of renin induced by dopamine is due to the activation of beta-receptors."}
{"id": "PMID:488281", "title": "Severe conditioned taste aversion elicited by venom of Russell's viper.", "content": "Conditioned taste aversion (CTA) established in rats by associating saccharin drinking with subsequent poisoning by Russell's viper venom. Retention test revealed 76 and 56% reduction of saccharin intake in venom and antivenom-venom groups. No CTA was observed in antivenom and control groups.", "contents": "Severe conditioned taste aversion elicited by venom of Russell's viper. Conditioned taste aversion (CTA) established in rats by associating saccharin drinking with subsequent poisoning by Russell's viper venom. Retention test revealed 76 and 56% reduction of saccharin intake in venom and antivenom-venom groups. No CTA was observed in antivenom and control groups."}
{"id": "PMID:488282", "title": "The localization of [3H]-desipramine in central nerve terminals studied with electron microscope autoradiography and subcellular fractionation.", "content": "The cellular and subcellular distribution of [3H]-desipramine (DMI) in rat brain was studied by electron microscope (EM) autoradiography and by subcellular fractionation. A considerable proportion of label was found to be bound to the membranes of presynaptic nerve terminals, as well as to sites inside those terminals.", "contents": "The localization of [3H]-desipramine in central nerve terminals studied with electron microscope autoradiography and subcellular fractionation. The cellular and subcellular distribution of [3H]-desipramine (DMI) in rat brain was studied by electron microscope (EM) autoradiography and by subcellular fractionation. A considerable proportion of label was found to be bound to the membranes of presynaptic nerve terminals, as well as to sites inside those terminals."}
{"id": "PMID:488283", "title": "Dissociation constants of 4-acetoxy-piperidines and -thiacyclohexanes at the muscarinic receptor.", "content": "The dissociation constants (KA) and the relative intrinsic efficacies (e) of 4-acetoxy-piperidines and -thiacyclohexanes were determined on the guinea-pig isolated left atrium. The differences in the muscarinic potencies are associated with differences in affinities and efficacies, respectively.", "contents": "Dissociation constants of 4-acetoxy-piperidines and -thiacyclohexanes at the muscarinic receptor. The dissociation constants (KA) and the relative intrinsic efficacies (e) of 4-acetoxy-piperidines and -thiacyclohexanes were determined on the guinea-pig isolated left atrium. The differences in the muscarinic potencies are associated with differences in affinities and efficacies, respectively."}
{"id": "PMID:488284", "title": "Protein synthesis inhibition induced by dimethylnitrosamine and diethylnitrosamine on isolated rat hepatocytes.", "content": "Time- and dose-dependent protein synthesis inhibition takes place following exposure to high doses of dimethylnitrosamine (DMN) or diethylnitrosamine (DENA) in isolated rat hepatocytes. The ability of DENA to depress protein synthesis is 5-fold higher than that of DMN. Cells inhibited by 60 min exposure to DMN or DENA, and then incubated in a nitrosamine-free medium, regain their initial rate of protein synthesis. This recovery is faster and more complete for DENA-treated cells.", "contents": "Protein synthesis inhibition induced by dimethylnitrosamine and diethylnitrosamine on isolated rat hepatocytes. Time- and dose-dependent protein synthesis inhibition takes place following exposure to high doses of dimethylnitrosamine (DMN) or diethylnitrosamine (DENA) in isolated rat hepatocytes. The ability of DENA to depress protein synthesis is 5-fold higher than that of DMN. Cells inhibited by 60 min exposure to DMN or DENA, and then incubated in a nitrosamine-free medium, regain their initial rate of protein synthesis. This recovery is faster and more complete for DENA-treated cells."}
{"id": "PMID:488285", "title": "Structural alterations in normal and axotomized facial nucleus neurons after treatment with morphine.", "content": "Chronic morphine administration induced ultrastructural alterations in neurons of the facial nucleus and increased the incidence of cell death after axotomy. These findings may correlate with the significant depression of neuronal metabolism known to occur after opiate treatment.", "contents": "Structural alterations in normal and axotomized facial nucleus neurons after treatment with morphine. Chronic morphine administration induced ultrastructural alterations in neurons of the facial nucleus and increased the incidence of cell death after axotomy. These findings may correlate with the significant depression of neuronal metabolism known to occur after opiate treatment."}
{"id": "PMID:488286", "title": "Ouabain potentiation of Ca release from fragmented cardiac sarcoplasmic reticulum from isolated cat heart.", "content": "The present study was performed to determine the effect of ouabain on Ca release from fragmented sarcoplasmic reticulum (FSR) isolated from cat cardiac muscles. The results clearly demonstrate that ouabain potentiates the Ca release from FSR by changing the ionic environment.", "contents": "Ouabain potentiation of Ca release from fragmented cardiac sarcoplasmic reticulum from isolated cat heart. The present study was performed to determine the effect of ouabain on Ca release from fragmented sarcoplasmic reticulum (FSR) isolated from cat cardiac muscles. The results clearly demonstrate that ouabain potentiates the Ca release from FSR by changing the ionic environment."}
{"id": "PMID:488287", "title": "Purification and properties of heat-resistant exotoxin produced by Macrophomina phaseolina (Tassi) Goid in culture.", "content": "A partially purified preparation of a water-soluble, heat-resistant, nonspecific exotoxin produced by a strain of Macrophomina phaseolina, isolated from Phaseolus mungo L. could reduce Cu++ ions and produced a red colour with 2,4-dinitrophenyl hydrazine reagent. It caused inhibition of seed germination, wilting of cult seedlings, stunted growth of young seedlings and loss of permeability of the cell membrane. Seedlings of P. mungo, grown in presence of the toxin showed a slight increase in the contents of protein and total RNA over control, but a significant increase in the specific activities of F-1, 6-BP aldolase and G-6-P isomerase.", "contents": "Purification and properties of heat-resistant exotoxin produced by Macrophomina phaseolina (Tassi) Goid in culture. A partially purified preparation of a water-soluble, heat-resistant, nonspecific exotoxin produced by a strain of Macrophomina phaseolina, isolated from Phaseolus mungo L. could reduce Cu++ ions and produced a red colour with 2,4-dinitrophenyl hydrazine reagent. It caused inhibition of seed germination, wilting of cult seedlings, stunted growth of young seedlings and loss of permeability of the cell membrane. Seedlings of P. mungo, grown in presence of the toxin showed a slight increase in the contents of protein and total RNA over control, but a significant increase in the specific activities of F-1, 6-BP aldolase and G-6-P isomerase."}
{"id": "PMID:488288", "title": "Changes in serum proteins and in major liver constituents following carbon tetrachloride and a high-fat choline-free diet in rats.", "content": "Both carbon tetrachloride and a high-fat, low-protein choline-free diet cause hepatomegaly in rats due to an accumulation of both lipids and proteins with a concomitant increase in deoxyribonucleic acid content. CCl4 causes a decline in hepatic glycogen. Serum albumin level is decreased in CCl4-treated rats and increased in those fed the experimental diet.", "contents": "Changes in serum proteins and in major liver constituents following carbon tetrachloride and a high-fat choline-free diet in rats. Both carbon tetrachloride and a high-fat, low-protein choline-free diet cause hepatomegaly in rats due to an accumulation of both lipids and proteins with a concomitant increase in deoxyribonucleic acid content. CCl4 causes a decline in hepatic glycogen. Serum albumin level is decreased in CCl4-treated rats and increased in those fed the experimental diet."}
{"id": "PMID:488289", "title": "Collagenolytic activity of eosinophilic granuloma in vitro.", "content": "Lytic activity of eosinophilic granuloma and other tumours was studied in vitro on collagen substrate. Collagen degradation was measured through the release of hydroxyproline-rich peptides into the medium. The in vitro lytic action was at a maximum in the case of EG and was correlated with the presence of histiocytic cells.", "contents": "Collagenolytic activity of eosinophilic granuloma in vitro. Lytic activity of eosinophilic granuloma and other tumours was studied in vitro on collagen substrate. Collagen degradation was measured through the release of hydroxyproline-rich peptides into the medium. The in vitro lytic action was at a maximum in the case of EG and was correlated with the presence of histiocytic cells."}
{"id": "PMID:488290", "title": "Ultrastructural localization of vanadium in the blood cells of Ascidiacea.", "content": "X-ray histospectrographic analysis at the scanning and transmission electron microscope (STEM) are made on the blood cells of Phallusia mamillata Cuvier and Ciona intestinalis, to study the 'direct' intracellular sites of accumulation of vanadium. The results show a clear accumulation of vanadium on the membrane and in the granules of vacuoles of amebocytes, signet ring cell, compartment cell and traces of metal in the 'vanadophores' of vanadocytes.", "contents": "Ultrastructural localization of vanadium in the blood cells of Ascidiacea. X-ray histospectrographic analysis at the scanning and transmission electron microscope (STEM) are made on the blood cells of Phallusia mamillata Cuvier and Ciona intestinalis, to study the 'direct' intracellular sites of accumulation of vanadium. The results show a clear accumulation of vanadium on the membrane and in the granules of vacuoles of amebocytes, signet ring cell, compartment cell and traces of metal in the 'vanadophores' of vanadocytes."}
{"id": "PMID:488291", "title": "A histochemical study on the innervation of the cerebral blood vessels in the carp.", "content": "Adrenergic and cholinergic innervation of the cerebral blood vessels were studied in the carp using fluorescence and cholinesterase histochemistry. Most of the major cerebral and pial arteries were densely innervated with both nerves. Moreover, both nerves, being probably of central origin, were observed frequently on the walls of parenchymal arterioles and capillaries in the sections of the inferior lobe but were only rarely found in the other brain sections.", "contents": "A histochemical study on the innervation of the cerebral blood vessels in the carp. Adrenergic and cholinergic innervation of the cerebral blood vessels were studied in the carp using fluorescence and cholinesterase histochemistry. Most of the major cerebral and pial arteries were densely innervated with both nerves. Moreover, both nerves, being probably of central origin, were observed frequently on the walls of parenchymal arterioles and capillaries in the sections of the inferior lobe but were only rarely found in the other brain sections."}
{"id": "PMID:488292", "title": "Patterns of neuroglial proliferation in spinal cord white matter following exposure to ionizing radiation.", "content": "X-irradiation temporarily decreases the proliferative activity of neuroglia in immature rat spinal cord. Later, the proliferative activity in these irradiated regions surpasses that noted in control rats. Areas adjacent to the irradiated region have a greater than normal percentage of labelled neuroglia and may also be a source of neuroglia which re-populate the irradiated zone.", "contents": "Patterns of neuroglial proliferation in spinal cord white matter following exposure to ionizing radiation. X-irradiation temporarily decreases the proliferative activity of neuroglia in immature rat spinal cord. Later, the proliferative activity in these irradiated regions surpasses that noted in control rats. Areas adjacent to the irradiated region have a greater than normal percentage of labelled neuroglia and may also be a source of neuroglia which re-populate the irradiated zone."}
{"id": "PMID:488293", "title": "Regional vascular reactivity in rats with Gu\u00e9rin carcinoma (GC).", "content": "Increased sensitivity to phenylephrin and a decreased one to isoproterenol has been found in regional vascular beds of GC implanted rats. Circulatory sympathetic reflex adaptation, alfa- and beta-adrenergic stimulation or blockade provoked a uniform response pattern: i.e. increase in vascular resistance of GC.", "contents": "Regional vascular reactivity in rats with Gu\u00e9rin carcinoma (GC). Increased sensitivity to phenylephrin and a decreased one to isoproterenol has been found in regional vascular beds of GC implanted rats. Circulatory sympathetic reflex adaptation, alfa- and beta-adrenergic stimulation or blockade provoked a uniform response pattern: i.e. increase in vascular resistance of GC."}
{"id": "PMID:488294", "title": "Nuclear localization of aldosterone in rat brain cells assessed by autoradiography.", "content": "Autoradiographic studies with 3H aldosterone demonstrate nuclear concentration of hormone in neurons of the hippocampus, septum, allocortical regions and brain stem reticular formation and motor nuclei of cranial nerves and in the meninges. The results suggest that mineralocorticoids have wide ranging effects on different parts of the central nervous system.", "contents": "Nuclear localization of aldosterone in rat brain cells assessed by autoradiography. Autoradiographic studies with 3H aldosterone demonstrate nuclear concentration of hormone in neurons of the hippocampus, septum, allocortical regions and brain stem reticular formation and motor nuclei of cranial nerves and in the meninges. The results suggest that mineralocorticoids have wide ranging effects on different parts of the central nervous system."}
{"id": "PMID:488295", "title": "Restoration of the poor oxygen transport function of ACD-stored blood by pyridoxal 5'-phosphate.", "content": "Pyridoxal 5'-phosphate is easily incorporated into ACD-stored erythrocytes without decrease of ATP, and restores the poor oxygen transport function with a similar effect to 2,3'-diphosphoglycerate.", "contents": "Restoration of the poor oxygen transport function of ACD-stored blood by pyridoxal 5'-phosphate. Pyridoxal 5'-phosphate is easily incorporated into ACD-stored erythrocytes without decrease of ATP, and restores the poor oxygen transport function with a similar effect to 2,3'-diphosphoglycerate."}
{"id": "PMID:488296", "title": "Cerebrospinal fluid immune complexes in multiple sclerosis.", "content": "Using a C1q binding test, immune complexes have been detected in one half of cerebrospinal fluid samples from patients with multiple sclerosis. These results provide additional evidence for the participation of an immune reaction in the disease process.", "contents": "Cerebrospinal fluid immune complexes in multiple sclerosis. Using a C1q binding test, immune complexes have been detected in one half of cerebrospinal fluid samples from patients with multiple sclerosis. These results provide additional evidence for the participation of an immune reaction in the disease process."}
{"id": "PMID:488297", "title": "Conversion of glycine max seed agglutinins from nonspecific to anti-(A + B).", "content": "The seeds of glycine max contain agglutinins which are typically nonspecific in their reactivity. Our investigations show that the phytagglutinins in GM can be converted from nonspecific to anti-(A + B) after the lectin is absorbed with horse red cells. The anti-A and anti-B fractions can be further separated by suitably absorbing the lectin with human red cells. The lectin absorbed with horse red cells or with group-0 human red cells shows an A-stressed activity.", "contents": "Conversion of glycine max seed agglutinins from nonspecific to anti-(A + B). The seeds of glycine max contain agglutinins which are typically nonspecific in their reactivity. Our investigations show that the phytagglutinins in GM can be converted from nonspecific to anti-(A + B) after the lectin is absorbed with horse red cells. The anti-A and anti-B fractions can be further separated by suitably absorbing the lectin with human red cells. The lectin absorbed with horse red cells or with group-0 human red cells shows an A-stressed activity."}
{"id": "PMID:488298", "title": "Changes in the sialic acid content of chick thymus and bursa of Fabricius during age-involution.", "content": "The sialic acid content of both thymus and bursa of Fabricius during their growth and involution phases in chick has been reported in this study. It is observed that the sialic acid concentration is very high in 1-week-old chickens. The concentration subsequently decreases to a significant level and rises again prior to the onset of involution. In the post-involution period, a more or less minimal and constant level is maintained. The role of sialic acid in cellular activities of thymo-bursal system has been discussed.", "contents": "Changes in the sialic acid content of chick thymus and bursa of Fabricius during age-involution. The sialic acid content of both thymus and bursa of Fabricius during their growth and involution phases in chick has been reported in this study. It is observed that the sialic acid concentration is very high in 1-week-old chickens. The concentration subsequently decreases to a significant level and rises again prior to the onset of involution. In the post-involution period, a more or less minimal and constant level is maintained. The role of sialic acid in cellular activities of thymo-bursal system has been discussed."}
{"id": "PMID:488299", "title": "Radioimmunoassay of an invertebrate peptide hormone - the crustacean neurosecretory hyperglycemic hormone.", "content": "Antibodies against hyperglycemic hormone (CHH) of Carcinus were raised in rabbits by injection of extract from sinus glands which contain high concentrations of CHH. The antiserum neutralizes the biological activity of CHH and binds 125-J-CHH. A RIA for CHH was established and was used to measure the hormone content of sinus glands.", "contents": "Radioimmunoassay of an invertebrate peptide hormone - the crustacean neurosecretory hyperglycemic hormone. Antibodies against hyperglycemic hormone (CHH) of Carcinus were raised in rabbits by injection of extract from sinus glands which contain high concentrations of CHH. The antiserum neutralizes the biological activity of CHH and binds 125-J-CHH. A RIA for CHH was established and was used to measure the hormone content of sinus glands."}
{"id": "PMID:488300", "title": "DNA synthesis in the pituitary gland of the rat: effect of sulpiride and clomiphene.", "content": "Sulpiride administration to rats releases prolactin and increases DNA replication in the anterior pituitary gland. Clomiphene prevents the stimulation of DNA synthesis produced by sulpiride, but does not affect prolactin release from the gland. These findings suggest that the intracellular prolactin content of the anterior pituitary gland plays a role in the regulation of DNA synthesis through a mechanism mediated by oestrogens.", "contents": "DNA synthesis in the pituitary gland of the rat: effect of sulpiride and clomiphene. Sulpiride administration to rats releases prolactin and increases DNA replication in the anterior pituitary gland. Clomiphene prevents the stimulation of DNA synthesis produced by sulpiride, but does not affect prolactin release from the gland. These findings suggest that the intracellular prolactin content of the anterior pituitary gland plays a role in the regulation of DNA synthesis through a mechanism mediated by oestrogens."}
{"id": "PMID:488301", "title": "Substance P in medullary carcinoma of the thyroid.", "content": "High levels of substance P-like immunoreactivity were demonstrated by radioimmunoassay in the plasma and tumour of a patient with a medullary carcinoma of the thyroid.", "contents": "Substance P in medullary carcinoma of the thyroid. High levels of substance P-like immunoreactivity were demonstrated by radioimmunoassay in the plasma and tumour of a patient with a medullary carcinoma of the thyroid."}
{"id": "PMID:488302", "title": "Effect of phosphate omission on glucose-induced insulin release in vitro.", "content": "In the isolated perfused rat pancreas, omission of extracellular phosphate (H2PO-4) significantly reduces the insulin secretion in response to 16.7 mM glucose.", "contents": "Effect of phosphate omission on glucose-induced insulin release in vitro. In the isolated perfused rat pancreas, omission of extracellular phosphate (H2PO-4) significantly reduces the insulin secretion in response to 16.7 mM glucose."}
{"id": "PMID:488303", "title": "Measurement of urinary steroid production rates using stable-isotopes and GC-MS.", "content": "The urinary production rate of pregnenolone has been determined for a male subject using 7,7-d2-pregnenolone as an isotopic tracer.", "contents": "Measurement of urinary steroid production rates using stable-isotopes and GC-MS. The urinary production rate of pregnenolone has been determined for a male subject using 7,7-d2-pregnenolone as an isotopic tracer."}
{"id": "PMID:488304", "title": "Oxygen consumption in the rat following neonatal thyrotoxicosis.", "content": "Adult rats made thyrotoxic with large doses of thyroxine during the neonatal period had a lower oxygen uptake as compared with neonatal controls and those of neonatal calorically-deprivated ones.", "contents": "Oxygen consumption in the rat following neonatal thyrotoxicosis. Adult rats made thyrotoxic with large doses of thyroxine during the neonatal period had a lower oxygen uptake as compared with neonatal controls and those of neonatal calorically-deprivated ones."}
{"id": "PMID:488305", "title": "A simple theoretical criterion of chemical carcinogenicity?", "content": "The simple theoretical criterion for chemical carcinogenicity based on the 'average quasi-valence number' is discussed and shown to be inappropriate as a reliable indicator of carcinogenicity of any organic compound.", "contents": "A simple theoretical criterion of chemical carcinogenicity? The simple theoretical criterion for chemical carcinogenicity based on the 'average quasi-valence number' is discussed and shown to be inappropriate as a reliable indicator of carcinogenicity of any organic compound."}
{"id": "PMID:488306", "title": "A demonstration of the resolution of NMR imaging in biological systems.", "content": "A proton NMR imaging study of several fruit specimens demonstrates the integrity and resolution of this new imaging method.", "contents": "A demonstration of the resolution of NMR imaging in biological systems. A proton NMR imaging study of several fruit specimens demonstrates the integrity and resolution of this new imaging method."}
{"id": "PMID:488307", "title": "Computer-compatible system for continuous volumetric measurement of water consumption?", "content": "An inexpensive drinkometer system is described that is compatible with most data acquisition systems and can be used for continuously monitoring the drinking pattern of laboratory animals. The change in electrical inductance resulting from the descent of a free-floating ferrite unit through a drinking tube, surrounded by a double coil, is converted to a signal proportional to the volume of fluid consumed.", "contents": "Computer-compatible system for continuous volumetric measurement of water consumption? An inexpensive drinkometer system is described that is compatible with most data acquisition systems and can be used for continuously monitoring the drinking pattern of laboratory animals. The change in electrical inductance resulting from the descent of a free-floating ferrite unit through a drinking tube, surrounded by a double coil, is converted to a signal proportional to the volume of fluid consumed."}
{"id": "PMID:488308", "title": "Training in family therapy: perceptual, conceptual and executive skills.", "content": "This paper presents a comprehensive and detailed outline of family therapy skills to aid in providing a more precise focus in the training of clinicians in family therapy. The skills are based on an integrated treatment model within a systems framework. Four major functions performed by a family therapist are separated and are further differentiated into general therapeutic competencies. Specific perceptual, conceptual, and executive skills are described in the form of instructional objectives and are listed under each competency. Occasional clarifying notes or examples are cited along with particular skills. Clinicians and trainees should find this outline a useful guide in skill development.", "contents": "Training in family therapy: perceptual, conceptual and executive skills. This paper presents a comprehensive and detailed outline of family therapy skills to aid in providing a more precise focus in the training of clinicians in family therapy. The skills are based on an integrated treatment model within a systems framework. Four major functions performed by a family therapist are separated and are further differentiated into general therapeutic competencies. Specific perceptual, conceptual, and executive skills are described in the form of instructional objectives and are listed under each competency. Occasional clarifying notes or examples are cited along with particular skills. Clinicians and trainees should find this outline a useful guide in skill development."}
{"id": "PMID:488309", "title": "Family treatment approaches to drug abuse problems: a review.", "content": "This review covers the literature that has emerged specifically on the family treatment of drug abuse problems. Following a brief discussion of patterns and structures prevalent in drug-abusing families, 68 different studies or programs (discussed in 74 papers) are compared as to their techniques and results. These are categorized within the following modalities: marital treatment, group treatment for parents, concurrent parent and identified patient treatment, treatment with individual families (both inpatient and outpatient), sibling-oriented treatment, multiple family therapy, and social network therapy. A table presents the various studies, along with the types of results they provide. Outcomes are contrasted for the 14 studies that quantified their results. The final section presents implications for the following areas: treatment activities (clarification of technique, family recruitment, direction and effectiveness of treatment, confidentiality, and treatment delivery systems), training, prevention, and future research (outcome, technique and responsibility). It is concluded that family treatment for drug abuse is gaining widespread acceptance and shows considerable promise for dealing effectively with problems of this type.", "contents": "Family treatment approaches to drug abuse problems: a review. This review covers the literature that has emerged specifically on the family treatment of drug abuse problems. Following a brief discussion of patterns and structures prevalent in drug-abusing families, 68 different studies or programs (discussed in 74 papers) are compared as to their techniques and results. These are categorized within the following modalities: marital treatment, group treatment for parents, concurrent parent and identified patient treatment, treatment with individual families (both inpatient and outpatient), sibling-oriented treatment, multiple family therapy, and social network therapy. A table presents the various studies, along with the types of results they provide. Outcomes are contrasted for the 14 studies that quantified their results. The final section presents implications for the following areas: treatment activities (clarification of technique, family recruitment, direction and effectiveness of treatment, confidentiality, and treatment delivery systems), training, prevention, and future research (outcome, technique and responsibility). It is concluded that family treatment for drug abuse is gaining widespread acceptance and shows considerable promise for dealing effectively with problems of this type."}
{"id": "PMID:488310", "title": "A family-oriented psychiatric inpatient unit.", "content": "This article presents the structure and policies of a psychiatric inpatient unit that was developed with the goal of fully integrating family-oriented treatment approaches into its therapeutic program. There is an explanation of different methods to involve families in the hospital treatment process and delineation of a variety of treatment techniques specific for families of inpatients. The role of the nursing staff is described as well as some of the contradictions and paradoxes that are inherent in this type of inpatient unit.", "contents": "A family-oriented psychiatric inpatient unit. This article presents the structure and policies of a psychiatric inpatient unit that was developed with the goal of fully integrating family-oriented treatment approaches into its therapeutic program. There is an explanation of different methods to involve families in the hospital treatment process and delineation of a variety of treatment techniques specific for families of inpatients. The role of the nursing staff is described as well as some of the contradictions and paradoxes that are inherent in this type of inpatient unit."}
{"id": "PMID:488311", "title": "Toward a metacommunicational framework of couple interactions.", "content": "A multi-level, metacommunicational framework to understand couple interactions is presented. Five interactional levels are defined following a mode of abstraction that parallels the theory of logical types; case examples are offered of couples interacting at each of the levels. The clinical implications of the framework, as a metaphor for understanding transactional processes, are discussed with an emphasis on the pragmatics of working with punctuational diffferences, developing therapeutic strategies, measuring progress, and setting goals for therapy with couples.", "contents": "Toward a metacommunicational framework of couple interactions. A multi-level, metacommunicational framework to understand couple interactions is presented. Five interactional levels are defined following a mode of abstraction that parallels the theory of logical types; case examples are offered of couples interacting at each of the levels. The clinical implications of the framework, as a metaphor for understanding transactional processes, are discussed with an emphasis on the pragmatics of working with punctuational diffferences, developing therapeutic strategies, measuring progress, and setting goals for therapy with couples."}
{"id": "PMID:488312", "title": "Structural and strategic approaches to psychosomatic families.", "content": "Patterns of relatedness characteristic of psychosomatic families are discussed. A step-wise intervention procedure based on structural and strategic approaches is presented. The goals of the procedure are to establish symptomatic relief and modify concurrently the patterns of relatedness. The procedure is applied to a sample of families in which a child presents with psychogenic abdominal pain. This paper is intended as a detailed and practical guide to working with such families; a degree of generalization is possible, as well.", "contents": "Structural and strategic approaches to psychosomatic families. Patterns of relatedness characteristic of psychosomatic families are discussed. A step-wise intervention procedure based on structural and strategic approaches is presented. The goals of the procedure are to establish symptomatic relief and modify concurrently the patterns of relatedness. The procedure is applied to a sample of families in which a child presents with psychogenic abdominal pain. This paper is intended as a detailed and practical guide to working with such families; a degree of generalization is possible, as well."}
{"id": "PMID:488313", "title": "A family myth: sex therapy gone awry.", "content": "The treatment of sexual disorders has achieved considerable popularity and respectability within the mental health profession and the lay community. As a consequence, it has become increasingly difficult to elude requests for this type of treatment and even more difficult to balance sexual concerns with the numerous other modes of relating that exist within a dyadic relationship. This paper will report two versions of a case study in which sexual dysfunction served as a myth that was carefully constructed to veil certain more fundamental problems.", "contents": "A family myth: sex therapy gone awry. The treatment of sexual disorders has achieved considerable popularity and respectability within the mental health profession and the lay community. As a consequence, it has become increasingly difficult to elude requests for this type of treatment and even more difficult to balance sexual concerns with the numerous other modes of relating that exist within a dyadic relationship. This paper will report two versions of a case study in which sexual dysfunction served as a myth that was carefully constructed to veil certain more fundamental problems."}
{"id": "PMID:488314", "title": "Family therapy as a treatment for children: a critical review of outcome research.", "content": "The value of family therapy as a treatment for child psychopathology is considered by reviewing pertinent outcome research. Fourteen studies that met three criteria are included in the review: (a) a child or adolescent was the identified patient or referral; (b) therapy included at least one parent and the child; and (c) outcome was evaluated in terms of the child's symptoms. There are major shortcomings in most of the available data, with only two well-controlled studies. Some empirical evidence does exist that family therapy is an effective treatment for children; the data from studies of adolescents are especially encouraging. However, insufficient data are available for comparing the relative merits of conjoint family treatment and individual child therapy. If the value of family therapy as a treatment alternative or, ideally, as the \"treatment of choice\" for a referred individual child is to be established, more and better controlled comparative outcome studies will be necessary. Suggestions for future research are presented emphasizing the need for a developmental perspective by recommending, for example, the use of factorial designs in which the intervenaction of treatment and age can be analyzed.", "contents": "Family therapy as a treatment for children: a critical review of outcome research. The value of family therapy as a treatment for child psychopathology is considered by reviewing pertinent outcome research. Fourteen studies that met three criteria are included in the review: (a) a child or adolescent was the identified patient or referral; (b) therapy included at least one parent and the child; and (c) outcome was evaluated in terms of the child's symptoms. There are major shortcomings in most of the available data, with only two well-controlled studies. Some empirical evidence does exist that family therapy is an effective treatment for children; the data from studies of adolescents are especially encouraging. However, insufficient data are available for comparing the relative merits of conjoint family treatment and individual child therapy. If the value of family therapy as a treatment alternative or, ideally, as the \"treatment of choice\" for a referred individual child is to be established, more and better controlled comparative outcome studies will be necessary. Suggestions for future research are presented emphasizing the need for a developmental perspective by recommending, for example, the use of factorial designs in which the intervenaction of treatment and age can be analyzed."}
{"id": "PMID:488316", "title": "[Effect of agents that act in the area of cholinergic synapses on the glycolysis process in an ischemic extremity].", "content": "Glycolysis intensity in an intact and ischemized muscle of a limb was decreased by prozerin and carbacholine in experiments on 230 noninbred rats with 2 models of acute ischemia. A less pronounced decrease was caused by benzohexonium, cholinesterase and dipyroxim. Meanwhile atropine blocked the glycolysis.", "contents": "[Effect of agents that act in the area of cholinergic synapses on the glycolysis process in an ischemic extremity]. Glycolysis intensity in an intact and ischemized muscle of a limb was decreased by prozerin and carbacholine in experiments on 230 noninbred rats with 2 models of acute ischemia. A less pronounced decrease was caused by benzohexonium, cholinesterase and dipyroxim. Meanwhile atropine blocked the glycolysis."}
{"id": "PMID:488317", "title": "[Effect of neurotropic agents on the healing of experimental mucosal erosions and on the noradrenaline content in the stomach wall].", "content": "In immobilized rats with hemorrhagic erosions of gastric mucosa induced by 8-hour electric stimulation, the neurotropic agents were injected intraperitoneally over three days after stimulation was discontinued. Phenamine and melipramine were conductive to healing of the mucous membrane erosions and produced no effect on the recovery of noradrenaline level in the intestinal wall. Clonidin and reserpin inhibited erosion healing and noradrenalline level recovery, whereas eterofen and atropin elicited no effect on the test parameters.", "contents": "[Effect of neurotropic agents on the healing of experimental mucosal erosions and on the noradrenaline content in the stomach wall]. In immobilized rats with hemorrhagic erosions of gastric mucosa induced by 8-hour electric stimulation, the neurotropic agents were injected intraperitoneally over three days after stimulation was discontinued. Phenamine and melipramine were conductive to healing of the mucous membrane erosions and produced no effect on the recovery of noradrenaline level in the intestinal wall. Clonidin and reserpin inhibited erosion healing and noradrenalline level recovery, whereas eterofen and atropin elicited no effect on the test parameters."}
{"id": "PMID:488318", "title": "[Effect of apressin on the contractility, blood supply and bioenergetics of the ischemic myocardium].", "content": "It has been shown that apressin in doses of 0.5 and 1 mg/kg intravenously prevented a drop of the amplitude of the left ventricular pressure, pressure, maximal speed of its rise and relaxation which developed in control experiments on dogs after ligation of the coronary artery. Apressin increased the volumetrical rate of the collateral coronary blood flow, diminished peripheral vascular resistance in the zone of myocardial ischemia and reduced the ST segment of the heart epicardial electrogram. The drug lowered glucose consumption, lactate content, and \"excess' lactate in blood samples collected from the ischemic zone. At succinate and glutamate oxidation, it decreased oxygen consumption by mitochondria from the myocardial ischemic zone in the acceptor-free system, and increased the respiration stimulation coefficient, the respiratory control alccording to Change being improved at glutamate oxidation.", "contents": "[Effect of apressin on the contractility, blood supply and bioenergetics of the ischemic myocardium]. It has been shown that apressin in doses of 0.5 and 1 mg/kg intravenously prevented a drop of the amplitude of the left ventricular pressure, pressure, maximal speed of its rise and relaxation which developed in control experiments on dogs after ligation of the coronary artery. Apressin increased the volumetrical rate of the collateral coronary blood flow, diminished peripheral vascular resistance in the zone of myocardial ischemia and reduced the ST segment of the heart epicardial electrogram. The drug lowered glucose consumption, lactate content, and \"excess' lactate in blood samples collected from the ischemic zone. At succinate and glutamate oxidation, it decreased oxygen consumption by mitochondria from the myocardial ischemic zone in the acceptor-free system, and increased the respiration stimulation coefficient, the respiratory control alccording to Change being improved at glutamate oxidation."}
{"id": "PMID:488320", "title": "[Effect of potassium glutamate on the manifestation of experimental arrhythmias in rabbits].", "content": "In rabbits and rats potassium glutaminate elicits antiarrhythmic effect in experimental arrhythmias induced by intravenous injection of strophanthine and calcium chloride, normalizes the content of electrolytes (potassium, sodium, calcium, magnesium) in the myocardium and blood of animals after they are injected strophanthine.", "contents": "[Effect of potassium glutamate on the manifestation of experimental arrhythmias in rabbits]. In rabbits and rats potassium glutaminate elicits antiarrhythmic effect in experimental arrhythmias induced by intravenous injection of strophanthine and calcium chloride, normalizes the content of electrolytes (potassium, sodium, calcium, magnesium) in the myocardium and blood of animals after they are injected strophanthine."}
{"id": "PMID:488321", "title": "[Cardiotropic effect of anti-arrhythmia agents].", "content": "In experiments on contractile proteins isolated from the myocardium rather than from the musculus gastrocnemius it was shown that in contradistinction to lidocain the drugs quinine, quinidine, novocainamide, papaverin, obsidan and isoptin at a concentration of 3 . 10(-4) M inhibit contraction of actomyosin threads and superprecipitation reaction of actomyosin suspension that occurs under the influence of adenosine triphosphoric acid.", "contents": "[Cardiotropic effect of anti-arrhythmia agents]. In experiments on contractile proteins isolated from the myocardium rather than from the musculus gastrocnemius it was shown that in contradistinction to lidocain the drugs quinine, quinidine, novocainamide, papaverin, obsidan and isoptin at a concentration of 3 . 10(-4) M inhibit contraction of actomyosin threads and superprecipitation reaction of actomyosin suspension that occurs under the influence of adenosine triphosphoric acid."}
{"id": "PMID:488319", "title": "[Action of potassium orotate, hyperbaric oxygenation and their combination on the course of experimental microfocal myocardial infarct].", "content": "The efficacy of potassium orotate (200 mg/kg), hyperbaric oxygenation (3 at. abs, totally six 50-minute sessions) and their combinations was studied in experiments on 150 rabbits. According to polarography, analysis of the level of nucleic acids and pathomorphological changes, the best effect can be achieved by combined use of potassium orotate and hyperbaric oxygenation. However, even under these circumstances the cardiac activity is not completely recovered on day 14 after the treatment is commenced.", "contents": "[Action of potassium orotate, hyperbaric oxygenation and their combination on the course of experimental microfocal myocardial infarct]. The efficacy of potassium orotate (200 mg/kg), hyperbaric oxygenation (3 at. abs, totally six 50-minute sessions) and their combinations was studied in experiments on 150 rabbits. According to polarography, analysis of the level of nucleic acids and pathomorphological changes, the best effect can be achieved by combined use of potassium orotate and hyperbaric oxygenation. However, even under these circumstances the cardiac activity is not completely recovered on day 14 after the treatment is commenced."}
{"id": "PMID:488324", "title": "[Spasmolytic properties of pyrrolizidine alkaloids].", "content": "Acute toxicity, spasmolytic and hypotensive properties of the alkaloids senecionine, d-otocenine and floridanin isolated from Senecio Erraticum L. were studied. D-otocenine and floridanin elicited low toxicity, while senecionine and d-otosenine showed spasmolytic and floridanin predominantly hypotensive properties.", "contents": "[Spasmolytic properties of pyrrolizidine alkaloids]. Acute toxicity, spasmolytic and hypotensive properties of the alkaloids senecionine, d-otocenine and floridanin isolated from Senecio Erraticum L. were studied. D-otocenine and floridanin elicited low toxicity, while senecionine and d-otosenine showed spasmolytic and floridanin predominantly hypotensive properties."}
{"id": "PMID:488325", "title": "[Pharmacological properties of a new benzimidazole derivative].", "content": "The pharmacological action of the new benziamidazole derivative 1-(3-diethylamino-2-oxypropyl)-2-propylbenzimidazole (abazol) was studied in experiments on mice, dogs, cats and isolated ears of rabbits. In cats and dogs, abazol produces a hypotensive effect and transient reduction of the EEG wave amplitude. Abazol dilates the vessels of an isolated rabbit ear. It shows less toxicity and less pronounced convulsant effect as compared to dibazol.", "contents": "[Pharmacological properties of a new benzimidazole derivative]. The pharmacological action of the new benziamidazole derivative 1-(3-diethylamino-2-oxypropyl)-2-propylbenzimidazole (abazol) was studied in experiments on mice, dogs, cats and isolated ears of rabbits. In cats and dogs, abazol produces a hypotensive effect and transient reduction of the EEG wave amplitude. Abazol dilates the vessels of an isolated rabbit ear. It shows less toxicity and less pronounced convulsant effect as compared to dibazol."}
{"id": "PMID:488326", "title": "[Effect of rheopolyglucin on the aggregation function of erythrocytes stored for different times].", "content": "The paper is concerned with changes in the aggregation function of red blood cells at rheopolyglucin dilution of the erythrocyte mass with varying dates of storage. Rheopolyglucin decreases aggregation of red blood cells. It has been also established that aggregation capacity of these cells is reduced on storage of conserved blood.", "contents": "[Effect of rheopolyglucin on the aggregation function of erythrocytes stored for different times]. The paper is concerned with changes in the aggregation function of red blood cells at rheopolyglucin dilution of the erythrocyte mass with varying dates of storage. Rheopolyglucin decreases aggregation of red blood cells. It has been also established that aggregation capacity of these cells is reduced on storage of conserved blood."}
{"id": "PMID:488322", "title": "[Mechanism of the anti-arrhythmia action of potassium and magnesium nicotinates].", "content": "It has been shown in rat experimental aconitinic arrhythmia and in dog postinfarction arrhythmia that combined potassium and magnesium nicotinates show a pronounced anti-arrhythmic effect. The studies on the drug distribution in the tissues indicate that the content of potassium, magnesium and total coenzymatic forms of nicotinic acid rises in the myocardium by the 12th hour from the commencement of the drug administration. Intracellular and exocellular content of potassium and sodium has been shown to return to normal.", "contents": "[Mechanism of the anti-arrhythmia action of potassium and magnesium nicotinates]. It has been shown in rat experimental aconitinic arrhythmia and in dog postinfarction arrhythmia that combined potassium and magnesium nicotinates show a pronounced anti-arrhythmic effect. The studies on the drug distribution in the tissues indicate that the content of potassium, magnesium and total coenzymatic forms of nicotinic acid rises in the myocardium by the 12th hour from the commencement of the drug administration. Intracellular and exocellular content of potassium and sodium has been shown to return to normal."}
{"id": "PMID:488329", "title": "[Effect of hydrocortisone on 14C-lysine incorporation into the nuclear and mitochondrial proteins of the rat thymus at different times of day].", "content": "The data on 14C-lysine incorporation have shown that normally there exists 12-hour periodicity of intensifying the synthesis of nuclear and mitochondrial proteins by the rat thymus, that correlates with similar changes in the DNA synthesis and in the mitotic rate. Administration of hydrocortisone during varying time of the day induces the synthesis of non-histone proteins of chromatin primarily in non-proliferating populations of thymus lymphocytes, the synthesis of histones being induced in proliferating ones.", "contents": "[Effect of hydrocortisone on 14C-lysine incorporation into the nuclear and mitochondrial proteins of the rat thymus at different times of day]. The data on 14C-lysine incorporation have shown that normally there exists 12-hour periodicity of intensifying the synthesis of nuclear and mitochondrial proteins by the rat thymus, that correlates with similar changes in the DNA synthesis and in the mitotic rate. Administration of hydrocortisone during varying time of the day induces the synthesis of non-histone proteins of chromatin primarily in non-proliferating populations of thymus lymphocytes, the synthesis of histones being induced in proliferating ones."}
{"id": "PMID:488327", "title": "[Pharmacokinetic characteristics of salicylates in old animals].", "content": "Experimental study of absorption, distribution, metabolism and excretion of salicylic acid and salicylate upon ageing showed that the sodium salicylate absorption rate is considerably decreased in old rats and rabbits. As a result, the plasma of old animals shows the low content of salicylates compared to adult ones. The level of salicylic acid in tissues and capacity of serum protein for salicylate binding are decreased. Excretion of salicylates in old rats was delayed compared to that in adult ones, the half-life in old rats was 2--3 times as long as that in adult species. The revealed features of salicylate pharmacokinetics in old animals confirm advisability of their further study in order to determine adequate doses of the drugs to be applied in geriatrics and to prevent their adverse on the ageing organism.", "contents": "[Pharmacokinetic characteristics of salicylates in old animals]. Experimental study of absorption, distribution, metabolism and excretion of salicylic acid and salicylate upon ageing showed that the sodium salicylate absorption rate is considerably decreased in old rats and rabbits. As a result, the plasma of old animals shows the low content of salicylates compared to adult ones. The level of salicylic acid in tissues and capacity of serum protein for salicylate binding are decreased. Excretion of salicylates in old rats was delayed compared to that in adult ones, the half-life in old rats was 2--3 times as long as that in adult species. The revealed features of salicylate pharmacokinetics in old animals confirm advisability of their further study in order to determine adequate doses of the drugs to be applied in geriatrics and to prevent their adverse on the ageing organism."}
{"id": "PMID:488330", "title": "[Acetylation and urinary excretion of norsulfazole in gastrointestinal disease patients of different age groups].", "content": "The amount of free and acetylated norsulfazol in the urine of patients with gastrointestinal diseases was analysed 3 hours after peroral administration of the drug (20 mg/kg). The acetylation rate of norsulfazol in patients of 21--30 years of age was significantly lower than that in heathy subjects of the same age. In groups of 31--40, 41--50 and 51--60 years of age the acetylation rate was 47, 42 and 43%, respectively. In patients with chronic cholecystitis excretion with urine of free norsulfazol was 2-fold as increased whereas in chronic gastritis there was a 2.7-fold increase as compared to controls. The acetylation rate of norsulfazole was 1.6, 1.4 and 1.5-fold reduced in duodenal ulcer, cholecystitis and gastritis, respectively.", "contents": "[Acetylation and urinary excretion of norsulfazole in gastrointestinal disease patients of different age groups]. The amount of free and acetylated norsulfazol in the urine of patients with gastrointestinal diseases was analysed 3 hours after peroral administration of the drug (20 mg/kg). The acetylation rate of norsulfazol in patients of 21--30 years of age was significantly lower than that in heathy subjects of the same age. In groups of 31--40, 41--50 and 51--60 years of age the acetylation rate was 47, 42 and 43%, respectively. In patients with chronic cholecystitis excretion with urine of free norsulfazol was 2-fold as increased whereas in chronic gastritis there was a 2.7-fold increase as compared to controls. The acetylation rate of norsulfazole was 1.6, 1.4 and 1.5-fold reduced in duodenal ulcer, cholecystitis and gastritis, respectively."}
{"id": "PMID:488332", "title": "[Antitumor and immunodepressive action of dexamethasone on a leukemia L-1210 model].", "content": "The effect of single administration of dexamethason in doses of 10, 20, 50 and 100 mg/kg on the spleen content of lymphomic colonies, normal colony-forming units and antibody-forming cells was studied in experiments on CBA and DBA/2 mice with transplanted leukemia L-1210. It has bear dependence on the dosage, the immunodepressant action of the drug being more pronounced in CBA mice as compared to DBA/2 mice with leukemia L-1210. The immunodepressant activity of dexamethason is approximately equal to the antineoplasic one. Single administration of the drug has practically no effect on hemopoiesis.", "contents": "[Antitumor and immunodepressive action of dexamethasone on a leukemia L-1210 model]. The effect of single administration of dexamethason in doses of 10, 20, 50 and 100 mg/kg on the spleen content of lymphomic colonies, normal colony-forming units and antibody-forming cells was studied in experiments on CBA and DBA/2 mice with transplanted leukemia L-1210. It has bear dependence on the dosage, the immunodepressant action of the drug being more pronounced in CBA mice as compared to DBA/2 mice with leukemia L-1210. The immunodepressant activity of dexamethason is approximately equal to the antineoplasic one. Single administration of the drug has practically no effect on hemopoiesis."}
{"id": "PMID:488328", "title": "[Effect of cyclophosphane on the function and structure of the kidneys].", "content": "In experiments on dogs and rats it has been shown that single administration of cyclophosphane in low doses produces no changes in the renal function or in water-electrolyte metabolism. Material changes in the canalicular apparatus of the kidneys, water-salt equilibrium and organ ultrastructure have been recorded in prolonged administration of the drug.", "contents": "[Effect of cyclophosphane on the function and structure of the kidneys]. In experiments on dogs and rats it has been shown that single administration of cyclophosphane in low doses produces no changes in the renal function or in water-electrolyte metabolism. Material changes in the canalicular apparatus of the kidneys, water-salt equilibrium and organ ultrastructure have been recorded in prolonged administration of the drug."}
{"id": "PMID:488334", "title": "[Effect of the prolonged exposure to etylene glycol and fatty acid ester on the functional state of the body of experimental animals].", "content": "The effect of prolonged (for 1 1/2 month) action of ethylene glycol and fatty acids ester on the bodily function was studied in experiments on rats by means of a complex of techniques. The threshold dose determined in a subacute experiment amounted to 1.8625 g/kg. The cumulative effect of the preparation is evaluated as little and medium cumulative.", "contents": "[Effect of the prolonged exposure to etylene glycol and fatty acid ester on the functional state of the body of experimental animals]. The effect of prolonged (for 1 1/2 month) action of ethylene glycol and fatty acids ester on the bodily function was studied in experiments on rats by means of a complex of techniques. The threshold dose determined in a subacute experiment amounted to 1.8625 g/kg. The cumulative effect of the preparation is evaluated as little and medium cumulative."}
{"id": "PMID:488331", "title": "[Human pharmacokinetic characteristics of sulfamonomethoxine depending on the drug form].", "content": "Comparative study of sulfamonomethoxin pharmacokinetics in the man given the drug per os or parenterally in the form of methylglucaminate showed that the latter favours higher blood concentrations of the drug 12 hours following injection. Sulfamonomethoxinmeglumin is absorbed more rapidly and has a greater bioavailability. The drug is mainly excreted with urine, more than a half being excreted in an acetylated form. It is recommended that the initial and maintenance doses be not less than 1000 and 500 mg for both dosage forms respectively. The interval between sulfanilamide administrations should not exceed 24 hours.", "contents": "[Human pharmacokinetic characteristics of sulfamonomethoxine depending on the drug form]. Comparative study of sulfamonomethoxin pharmacokinetics in the man given the drug per os or parenterally in the form of methylglucaminate showed that the latter favours higher blood concentrations of the drug 12 hours following injection. Sulfamonomethoxinmeglumin is absorbed more rapidly and has a greater bioavailability. The drug is mainly excreted with urine, more than a half being excreted in an acetylated form. It is recommended that the initial and maintenance doses be not less than 1000 and 500 mg for both dosage forms respectively. The interval between sulfanilamide administrations should not exceed 24 hours."}
{"id": "PMID:488333", "title": "[Immune and autoimmune reactions in the mechanism of action of drug substances, their detoxication and the development of habituation to them].", "content": "It has been shown that rausedyl administration is followed by enhanced synthesis of autoantibodies to serotonine and adrenaline. Meanwhile bucarban enhances the synthesis of autoantibodies to endogenous insulin and ACTH to hydrocortisone and testosterone. Activation of the autoimmune system of regulating the balance of endogenous compounds was shown by an increased number of specific antibody-producing cells in the organs and tissues and by the growth of the titer of circulating antibodies in the blood.", "contents": "[Immune and autoimmune reactions in the mechanism of action of drug substances, their detoxication and the development of habituation to them]. It has been shown that rausedyl administration is followed by enhanced synthesis of autoantibodies to serotonine and adrenaline. Meanwhile bucarban enhances the synthesis of autoantibodies to endogenous insulin and ACTH to hydrocortisone and testosterone. Activation of the autoimmune system of regulating the balance of endogenous compounds was shown by an increased number of specific antibody-producing cells in the organs and tissues and by the growth of the titer of circulating antibodies in the blood."}
{"id": "PMID:488369", "title": "The Na:K pump in red cells is electrogenic.", "content": "The membrane potential, E, of the red cell measured with a fluorescent dye, 3,3'-dipropylthiadicarbocyanine iodide, hyperpolarizes when the Na:K pump is activated by adding external K and depolarizes upon the subsequent addition of ouabain. The electrogenic pump is optimally observed in cells where internal Na+ has been raised, SO2-(4) has replaced Cl-, and SO2-(4) permeability has been inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS)). The change in E associated with the electrogenic component is about 6 mV in human red cells, somewhat smaller in sheep, and larger in duck and Amphiuma red cells. The membrane resistance, Rm, can be estimated from the pump-dependent change in E and from the current flow assumed to be one-third the ouabain-sensitive Na efflux. In human red cells, Rm is about 1 X 10(6) ohm-cm2. Rm calculated from the residual DIDS-insensitive SO2-(4) flux is also about 1 X 10(6) ohm-cm2. The closeness of these two values of Rm is paralleled in the other three types of red cells (even though the absolute values of Rm vary among the four types by a factor of 10), indicating that the net current flow across the membrane can be accounted for by the net transport of Na by the pump.", "contents": "The Na:K pump in red cells is electrogenic. The membrane potential, E, of the red cell measured with a fluorescent dye, 3,3'-dipropylthiadicarbocyanine iodide, hyperpolarizes when the Na:K pump is activated by adding external K and depolarizes upon the subsequent addition of ouabain. The electrogenic pump is optimally observed in cells where internal Na+ has been raised, SO2-(4) has replaced Cl-, and SO2-(4) permeability has been inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS)). The change in E associated with the electrogenic component is about 6 mV in human red cells, somewhat smaller in sheep, and larger in duck and Amphiuma red cells. The membrane resistance, Rm, can be estimated from the pump-dependent change in E and from the current flow assumed to be one-third the ouabain-sensitive Na efflux. In human red cells, Rm is about 1 X 10(6) ohm-cm2. Rm calculated from the residual DIDS-insensitive SO2-(4) flux is also about 1 X 10(6) ohm-cm2. The closeness of these two values of Rm is paralleled in the other three types of red cells (even though the absolute values of Rm vary among the four types by a factor of 10), indicating that the net current flow across the membrane can be accounted for by the net transport of Na by the pump."}
{"id": "PMID:488370", "title": "Euphorigenic drugs: effects on the reward pathways of the brain.", "content": "This report summarizes a number of experiments designed to examine the changes in the threshold for intracranial self-stimulation (ICSS) in the rat after the administration of morphine and a number of narcotic agonist-antagonists, as well as three nonnarcotic drugs that have extensive nonmedical use (cocaine, d-amphetamine, and phencyclidine). The results of these experiments clearly indicate that morphine lowers the threshold for ICSS and, furthermore, there appears to be little or no tolerance to this effect. The only mixed agonist-antagonist that consistently lowered the ICSS threshold was pentazocine. Cocaine, d-amphetamine, and to a lesser degree, phencyclidine also lowered the ICSS threshold. These results suggest that the abuse liability of these agents may be directly related to their ability to sensitize the neural substrate involved with natural reward.", "contents": "Euphorigenic drugs: effects on the reward pathways of the brain. This report summarizes a number of experiments designed to examine the changes in the threshold for intracranial self-stimulation (ICSS) in the rat after the administration of morphine and a number of narcotic agonist-antagonists, as well as three nonnarcotic drugs that have extensive nonmedical use (cocaine, d-amphetamine, and phencyclidine). The results of these experiments clearly indicate that morphine lowers the threshold for ICSS and, furthermore, there appears to be little or no tolerance to this effect. The only mixed agonist-antagonist that consistently lowered the ICSS threshold was pentazocine. Cocaine, d-amphetamine, and to a lesser degree, phencyclidine also lowered the ICSS threshold. These results suggest that the abuse liability of these agents may be directly related to their ability to sensitize the neural substrate involved with natural reward."}
{"id": "PMID:488372", "title": "Cerebral energy state, mitochondrial function, and redox state measurements in transient ischemia.", "content": "Earlier results are reviewed suggesting that transient pronounced, incomplete cerebral ischemia could be more deleterious for the recovery of brain tissue energy state than a complete interruption of the blood flow. Measurements of respiratory function of brain mitochondria, isolated after 30 min of either complete or incomplete ischemia, demonstrated a similar inhibition of respiratory activity and maximal phosphorylation rates in both situations. This inhibition was totally normalized during recirculation after complete ischemia while a further deterioration was found after incomplete ischemia. The in vivo alterations of the cortical tissue distribution of redox states during transient, incomplete ischemia (15--60 min) were measured using a flying spot fluorometer, which gives a real-time and on-line display of the tissue distribution of NADH and oxidized flavoprotein. A reoxidation in both systems was demonstrated during the recirculation period and the distribution of redox states showed no further heterogeneity in the postischemic period as compared to the preischemic distribution. It is concluded that reoxygenation of the brain tissue is possible even after long periods of incomplete ischemia. The normal distribution of redox states during recirculation suggests that mechanisms other than an impaired or inhomogeneous oxygen delivery during the postischemic period are responsible for the failure in recovery of mitochondrial function and tissue energy state.", "contents": "Cerebral energy state, mitochondrial function, and redox state measurements in transient ischemia. Earlier results are reviewed suggesting that transient pronounced, incomplete cerebral ischemia could be more deleterious for the recovery of brain tissue energy state than a complete interruption of the blood flow. Measurements of respiratory function of brain mitochondria, isolated after 30 min of either complete or incomplete ischemia, demonstrated a similar inhibition of respiratory activity and maximal phosphorylation rates in both situations. This inhibition was totally normalized during recirculation after complete ischemia while a further deterioration was found after incomplete ischemia. The in vivo alterations of the cortical tissue distribution of redox states during transient, incomplete ischemia (15--60 min) were measured using a flying spot fluorometer, which gives a real-time and on-line display of the tissue distribution of NADH and oxidized flavoprotein. A reoxidation in both systems was demonstrated during the recirculation period and the distribution of redox states showed no further heterogeneity in the postischemic period as compared to the preischemic distribution. It is concluded that reoxygenation of the brain tissue is possible even after long periods of incomplete ischemia. The normal distribution of redox states during recirculation suggests that mechanisms other than an impaired or inhomogeneous oxygen delivery during the postischemic period are responsible for the failure in recovery of mitochondrial function and tissue energy state."}
{"id": "PMID:488375", "title": "Recent advances in the in vivo and in vitro metabolism of retinoic acid.", "content": "Retinoic acid, a natural metabolite of retinol, has previously been shown to be capable of supporting growth and maintaining proper differentiation in epithelial tissues. Recently, investigation into the in vivo and in vitro metabolism of retinoic acid in hamsters, using both tracheal organ culture and subcellular preparations derived from intestinal mucosa, liver, and testis, has revealed the production of several metabolites more polar than the parent compound. Two of the early products of this metabolic pathway have been identified as 4-hydroxy- and 4-keto-retinoic acid. The formation of these metabolites is maximal in vitamin A-deficient hamsters that have been pretreated with retinoic acid and in vitamin A-normal animals. This fact, together with the decreased biological activity of the two compounds relative to retinoic acid in a tracheal organ culture assay, suggested that oxidative attack at carbon-four of the cyclohexenyl ring may be the first step in the elimination of retinoic acid from tissues. In addition, observations both in vivo and in vitro indicate that all-trans- and 13-cis-retinoic acid at low concentrations may be sharing a common metabolic pathway that includes an isomer of 4-keto-retinoic acid.", "contents": "Recent advances in the in vivo and in vitro metabolism of retinoic acid. Retinoic acid, a natural metabolite of retinol, has previously been shown to be capable of supporting growth and maintaining proper differentiation in epithelial tissues. Recently, investigation into the in vivo and in vitro metabolism of retinoic acid in hamsters, using both tracheal organ culture and subcellular preparations derived from intestinal mucosa, liver, and testis, has revealed the production of several metabolites more polar than the parent compound. Two of the early products of this metabolic pathway have been identified as 4-hydroxy- and 4-keto-retinoic acid. The formation of these metabolites is maximal in vitamin A-deficient hamsters that have been pretreated with retinoic acid and in vitamin A-normal animals. This fact, together with the decreased biological activity of the two compounds relative to retinoic acid in a tracheal organ culture assay, suggested that oxidative attack at carbon-four of the cyclohexenyl ring may be the first step in the elimination of retinoic acid from tissues. In addition, observations both in vivo and in vitro indicate that all-trans- and 13-cis-retinoic acid at low concentrations may be sharing a common metabolic pathway that includes an isomer of 4-keto-retinoic acid."}
{"id": "PMID:488376", "title": "Chemoprevention of cancer with retinoids.", "content": "The importance of retinoids for chemoprevention of cancer has received further emphasis with the finding that retinoids can suppress in vitro expression of the malignant phenotype, whether it be caused by chemical carcinogens, radiation, or viral transforming factors. Retinoids have been found to be potent inhibitors of the tumor-promoting effects of phorbol esters. Further advances in the chemistry of synthetic retinoids have led to the development of new agents that show less toxicity and better targeting to specific organ sites than natural retinoids. Synthetic retinoids have been particularly useful for prevention of bladder and breast cancer in experimental animals.", "contents": "Chemoprevention of cancer with retinoids. The importance of retinoids for chemoprevention of cancer has received further emphasis with the finding that retinoids can suppress in vitro expression of the malignant phenotype, whether it be caused by chemical carcinogens, radiation, or viral transforming factors. Retinoids have been found to be potent inhibitors of the tumor-promoting effects of phorbol esters. Further advances in the chemistry of synthetic retinoids have led to the development of new agents that show less toxicity and better targeting to specific organ sites than natural retinoids. Synthetic retinoids have been particularly useful for prevention of bladder and breast cancer in experimental animals."}
{"id": "PMID:488406", "title": "Hypercarotenemia in hypothalamic amenorrhea.", "content": "Six patients are described with hypothalamic amenorrhea and associated hypercarotenemia. The commencement of the hypothalamic amenorrhea followed weight reduction or stress in each case. The subjects were otherwise healthy and had none of the associated stigmata of anorexia nervosa. None of the subjects had ingested excessive quantities of vegetables or fruits rich in carotene. Although all six patients had elevated serum carotene levels, only three had clinically apparent yellow pigmentation of the skin. The exact mechanism responsible for hypercarotenemia in patients with hypothalamic amenorrhea is not apparent. Mobilization of lipid stores and catabolic changes occurring with weight-loss states appear to be related in some undefined way to the elevated carotene levels.", "contents": "Hypercarotenemia in hypothalamic amenorrhea. Six patients are described with hypothalamic amenorrhea and associated hypercarotenemia. The commencement of the hypothalamic amenorrhea followed weight reduction or stress in each case. The subjects were otherwise healthy and had none of the associated stigmata of anorexia nervosa. None of the subjects had ingested excessive quantities of vegetables or fruits rich in carotene. Although all six patients had elevated serum carotene levels, only three had clinically apparent yellow pigmentation of the skin. The exact mechanism responsible for hypercarotenemia in patients with hypothalamic amenorrhea is not apparent. Mobilization of lipid stores and catabolic changes occurring with weight-loss states appear to be related in some undefined way to the elevated carotene levels."}
{"id": "PMID:488407", "title": "New therapeutic approach to the hirsute patient.", "content": "Spironolactone, an aldosterone antagonist currently used in the treatment of hypertension, has numerous antiandrogenic side effects. Decreased production rates of testosterone in intact (N = 10) and hirsute (N = 6) women, respectively, were noted after short (7 days) or long-term (6 months) administration of the drug, 25 mg twice daily. A 50% diminution in the urinary 17-ketosteroid excretion was also observed after 6 months of therapy. Assessment of the treatment was done by the patients: a reduction in hair growth rate was initially observed after 3 to 4 months, followed by the appearance of a more downy type of new growth and a decrease in diameter of the hair shaft. It is concluded that this beneficial effect is obtained by a decrease in the testosterone production rate and probably through competitive inhibition of spironolactone and/or canrenone with the androgen receptor in human hair follicles.", "contents": "New therapeutic approach to the hirsute patient. Spironolactone, an aldosterone antagonist currently used in the treatment of hypertension, has numerous antiandrogenic side effects. Decreased production rates of testosterone in intact (N = 10) and hirsute (N = 6) women, respectively, were noted after short (7 days) or long-term (6 months) administration of the drug, 25 mg twice daily. A 50% diminution in the urinary 17-ketosteroid excretion was also observed after 6 months of therapy. Assessment of the treatment was done by the patients: a reduction in hair growth rate was initially observed after 3 to 4 months, followed by the appearance of a more downy type of new growth and a decrease in diameter of the hair shaft. It is concluded that this beneficial effect is obtained by a decrease in the testosterone production rate and probably through competitive inhibition of spironolactone and/or canrenone with the androgen receptor in human hair follicles."}
{"id": "PMID:488409", "title": "Subnormal postconceptional levels of prolactin do not interfere with the early events of human pregnancy.", "content": "To elucidate the role of bromocriptine treatment and serum prolactin levels in peri-implantational events, 30 healthy women asking for \"morning after\" type contraception were given bromocriptine. Treatment was started 30.6 +/- 18.4 (mean +/- SD) hours after unprotected intercourse and continued until the next menstruation. Blood samples were taken before and 1 and 2 weeks after initiation of treatment, and the samples were assayed for concentrations of prolactin, progesterone, human chorionic gonadotropin (hCG), and pregnancy-specific beta1-glycoprotein (PSBG) by radioimmunoassay. Prolactin concentrations fell significantly (P less than 0.001) from the pretreatment level of 11.4 +/- 7.9 ng/ml (mean +/- SD) to 2.8 +/- 2.3 ng/ml at 1 week and to 1.9 +/- 0.6 ng/ml after 2 weeks of treatment. Five women had demonstrable hCG in serum 8 to 15 days after unprotected intercourse, and one of them also had PSBG 14 days postcoitum. Pregnancy advanced normally until legal termination in three women, whereas menstruation-like bleeding ensued in two. Our results suggest that neither bromocriptine treatment nor a subnormal maternal serum prolactin level interferes with the early development of human pregnancy.", "contents": "Subnormal postconceptional levels of prolactin do not interfere with the early events of human pregnancy. To elucidate the role of bromocriptine treatment and serum prolactin levels in peri-implantational events, 30 healthy women asking for \"morning after\" type contraception were given bromocriptine. Treatment was started 30.6 +/- 18.4 (mean +/- SD) hours after unprotected intercourse and continued until the next menstruation. Blood samples were taken before and 1 and 2 weeks after initiation of treatment, and the samples were assayed for concentrations of prolactin, progesterone, human chorionic gonadotropin (hCG), and pregnancy-specific beta1-glycoprotein (PSBG) by radioimmunoassay. Prolactin concentrations fell significantly (P less than 0.001) from the pretreatment level of 11.4 +/- 7.9 ng/ml (mean +/- SD) to 2.8 +/- 2.3 ng/ml at 1 week and to 1.9 +/- 0.6 ng/ml after 2 weeks of treatment. Five women had demonstrable hCG in serum 8 to 15 days after unprotected intercourse, and one of them also had PSBG 14 days postcoitum. Pregnancy advanced normally until legal termination in three women, whereas menstruation-like bleeding ensued in two. Our results suggest that neither bromocriptine treatment nor a subnormal maternal serum prolactin level interferes with the early development of human pregnancy."}
{"id": "PMID:488410", "title": "Mode of action of DL-norgestrel and ethinylestradiol combination in postcoital contraception.", "content": "Possible mechanisms of action of a combination of ethinylestradiol (EE) and dl-norgestrel as a postcoital contraceptive agent were studied in 12 healthy female volunteers. An oral dose of 0.1 mg of EE and 1.0 mg of dl-norgestrel was given at the predicted time of ovulation and again 12 hours later. Serum luteinizing hormone, prolactin, progesterone, 17 alpha-hydroxyprogesterone, and estradiol were measured by specific radioimmunoassays in blood samples obtained daily from the 8th day of the menstrual cycle to the 1st day of menses. Hormone profiles suggested that the medication elicited a range of individual variations in pituitary and/or ovarian responses. Histologic examination of the endometrium consistently showed significant alteration in endometrial development with a dissociation in maturation of glandular and stomal components. This postcoital contraceptive acts either by (1) suppressing ovulation or (2) disrupting luteal function by acting directly on the corpus luteum or by interfering with appropriate endometrial responses to ovarian steroids.", "contents": "Mode of action of DL-norgestrel and ethinylestradiol combination in postcoital contraception. Possible mechanisms of action of a combination of ethinylestradiol (EE) and dl-norgestrel as a postcoital contraceptive agent were studied in 12 healthy female volunteers. An oral dose of 0.1 mg of EE and 1.0 mg of dl-norgestrel was given at the predicted time of ovulation and again 12 hours later. Serum luteinizing hormone, prolactin, progesterone, 17 alpha-hydroxyprogesterone, and estradiol were measured by specific radioimmunoassays in blood samples obtained daily from the 8th day of the menstrual cycle to the 1st day of menses. Hormone profiles suggested that the medication elicited a range of individual variations in pituitary and/or ovarian responses. Histologic examination of the endometrium consistently showed significant alteration in endometrial development with a dissociation in maturation of glandular and stomal components. This postcoital contraceptive acts either by (1) suppressing ovulation or (2) disrupting luteal function by acting directly on the corpus luteum or by interfering with appropriate endometrial responses to ovarian steroids."}
{"id": "PMID:488411", "title": "Correlations between seminal radioimmunoreactive prolactin, sperm count, and sperm motility in prevasectomy and infertility clinic patients.", "content": "Semen samples were collected from 50 prevasectomy patients and 43 infertility clinic patients. Various parameters were measured, including semen radioimmunoreactive prolactin (rPRL) levels. Linear correlations were tested between the measured parameters, and a significant correlation was found between semen rPRL concentration and sperm count for both the prevasectomy (r = 0.73, P less than 0.00002) and the clinic patients (r = 0.72, P less than 0.00002). For the clinic patients a correlation was also found between the amount of ejaculate rPRL and sperm motility (r = 0.46, P less than 0.002) and between ejaculate volume and motility (r = 0.36, P less than 0.02).", "contents": "Correlations between seminal radioimmunoreactive prolactin, sperm count, and sperm motility in prevasectomy and infertility clinic patients. Semen samples were collected from 50 prevasectomy patients and 43 infertility clinic patients. Various parameters were measured, including semen radioimmunoreactive prolactin (rPRL) levels. Linear correlations were tested between the measured parameters, and a significant correlation was found between semen rPRL concentration and sperm count for both the prevasectomy (r = 0.73, P less than 0.00002) and the clinic patients (r = 0.72, P less than 0.00002). For the clinic patients a correlation was also found between the amount of ejaculate rPRL and sperm motility (r = 0.46, P less than 0.002) and between ejaculate volume and motility (r = 0.36, P less than 0.02)."}
{"id": "PMID:488413", "title": "Laser light-scattering study of the effect of progesterone on sperm motility.", "content": "It is known that progestins can induce in the secretory cells of the cervix the excretion of a mucus that is highly viscuos, scanty, and impenetrable to spermatozoa. Mucus of this type is similar to that excreted during the luteal phase of the normal human menstrual cycle and the cow estrous cycle. It is a natural sequence to ask the question, do progestins also have a direct effect on sperm motility? With dynamic laser light-scattering we measured the motility of freshly washed human spermatozoa and of spermatozoa in the presence of a progesterone, both in terms of their swimming speed distribution as expressed in the spectrum of scattered light. The swimming speed was significantly reduced when the concentration of progesterone was three orders of magnitude greater than that of the physiologic level. This finding confirms the finding in earlier biochemical studies that progesterone has a distinct spermiostatic effect. We suggest this answer to the above question: progestin-releasing contraceptive devices may act on spermatozoa directly as well as in the secretory cells of the cervix.", "contents": "Laser light-scattering study of the effect of progesterone on sperm motility. It is known that progestins can induce in the secretory cells of the cervix the excretion of a mucus that is highly viscuos, scanty, and impenetrable to spermatozoa. Mucus of this type is similar to that excreted during the luteal phase of the normal human menstrual cycle and the cow estrous cycle. It is a natural sequence to ask the question, do progestins also have a direct effect on sperm motility? With dynamic laser light-scattering we measured the motility of freshly washed human spermatozoa and of spermatozoa in the presence of a progesterone, both in terms of their swimming speed distribution as expressed in the spectrum of scattered light. The swimming speed was significantly reduced when the concentration of progesterone was three orders of magnitude greater than that of the physiologic level. This finding confirms the finding in earlier biochemical studies that progesterone has a distinct spermiostatic effect. We suggest this answer to the above question: progestin-releasing contraceptive devices may act on spermatozoa directly as well as in the secretory cells of the cervix."}
{"id": "PMID:488414", "title": "Vasovasostomy: evaluation of four surgical techniques.", "content": "Four techniques of vasovasostomy were evaluated in two groups of twelve dogs each. In group 1, one side was anastomosed by a standard technique over a Silastic stent and the other side was anastomosed using a two-layer microsurgical technique. In group 2, one side was anastomosed by a standard technique without a stent and the other side was anastomosed using a one-layer microsurgical technique. Patency was evaluated by methylene blue injection distal to the anastomosis and was found to be about the same for all three groups in which anastomosis was performed without stents. The stented group had a high rate of obstruction. Histologic evaluation, however, showed superiority of both microsurgical techniques over each of the stnadard procedures.", "contents": "Vasovasostomy: evaluation of four surgical techniques. Four techniques of vasovasostomy were evaluated in two groups of twelve dogs each. In group 1, one side was anastomosed by a standard technique over a Silastic stent and the other side was anastomosed using a two-layer microsurgical technique. In group 2, one side was anastomosed by a standard technique without a stent and the other side was anastomosed using a one-layer microsurgical technique. Patency was evaluated by methylene blue injection distal to the anastomosis and was found to be about the same for all three groups in which anastomosis was performed without stents. The stented group had a high rate of obstruction. Histologic evaluation, however, showed superiority of both microsurgical techniques over each of the stnadard procedures."}
{"id": "PMID:488415", "title": "Temporary sterility induced by intrascrotal deposition of silastic-polyvinylpyrrolidone-prostaglandin F2alpha tubes in the rabbit: effect on fetal survival after regain of fertility.", "content": "In male rabbits of proven fertility, the intrascrotal deposition of two Silastic-polyvinylpyrrolidone tubes containing 3 mg of prostaglandin F2alpha (PGF2alpha)/tube induced within 2 to 4 weeks temporary sterility which lasted for 10 to 14 weeks. Associated with induced sterility were reduction in testicular weight, increase in abnormal spermatozoa (8% to 78% versus 0 to 3.7%), and reduction in sperm motility along the reproductive tract and in the semen for a period of 6 to 7 weeks. During the period of temporary sterility the weight of the epididymis, the sexual drive, and semen volume remained unaltered. Normal fertility was associated with an increase in testicular weight, reduction in the proportion of abnormal sperm, and improved sperm motility. In addition to an altered spermatogenesis, the integrity of mature spermatozoa seemed to be severely affected after PGF2alpha treatment. The reduced number of viable young sired by males that recovered from temporary sterility (pregnancy wastage 35%) as compared with sham-treated controls (pregnancy wastage 3%) suggests that a small percentage of spermatozoa might still be defective at the time of testing. A longer waiting period might be needed to ensure a completely normal reproductive process.", "contents": "Temporary sterility induced by intrascrotal deposition of silastic-polyvinylpyrrolidone-prostaglandin F2alpha tubes in the rabbit: effect on fetal survival after regain of fertility. In male rabbits of proven fertility, the intrascrotal deposition of two Silastic-polyvinylpyrrolidone tubes containing 3 mg of prostaglandin F2alpha (PGF2alpha)/tube induced within 2 to 4 weeks temporary sterility which lasted for 10 to 14 weeks. Associated with induced sterility were reduction in testicular weight, increase in abnormal spermatozoa (8% to 78% versus 0 to 3.7%), and reduction in sperm motility along the reproductive tract and in the semen for a period of 6 to 7 weeks. During the period of temporary sterility the weight of the epididymis, the sexual drive, and semen volume remained unaltered. Normal fertility was associated with an increase in testicular weight, reduction in the proportion of abnormal sperm, and improved sperm motility. In addition to an altered spermatogenesis, the integrity of mature spermatozoa seemed to be severely affected after PGF2alpha treatment. The reduced number of viable young sired by males that recovered from temporary sterility (pregnancy wastage 35%) as compared with sham-treated controls (pregnancy wastage 3%) suggests that a small percentage of spermatozoa might still be defective at the time of testing. A longer waiting period might be needed to ensure a completely normal reproductive process."}
{"id": "PMID:488421", "title": "A comparison of gross and microsurgical techniques for repair of cornual occlusion in infertility: a retrospective study, 1968-1978.", "content": "A 10-year experience is recounted moving from traditional gross implantation methods to microsurgical anastomosis in the repair of cornual occlusion in infertility. Two gross surgical implantation techniques, one involving reaming out the intramural portion of the uterine tube and the other dissecting it out via a transfundal incision, are compared with microsurgical uterotubal anastomosis. The author reports a 4.7 times improvement in the term pregnancy rate after turning to microsurgery and describes his method for anastomosing the transected ampulla or isthmus to the intramural portion of the tube.", "contents": "A comparison of gross and microsurgical techniques for repair of cornual occlusion in infertility: a retrospective study, 1968-1978. A 10-year experience is recounted moving from traditional gross implantation methods to microsurgical anastomosis in the repair of cornual occlusion in infertility. Two gross surgical implantation techniques, one involving reaming out the intramural portion of the uterine tube and the other dissecting it out via a transfundal incision, are compared with microsurgical uterotubal anastomosis. The author reports a 4.7 times improvement in the term pregnancy rate after turning to microsurgery and describes his method for anastomosing the transected ampulla or isthmus to the intramural portion of the tube."}
{"id": "PMID:488422", "title": "An analysis of macrosurgical and microsurgical techniques in the management of the tuboperitoneal factor in infertility.", "content": "One hundred and sixty tubal reconstructions were analyzed according to a classification which describes the type of tubal repair and the tubal segment treated. The results in 80 women who were operated upon just prior to the application of microsurgical principles were compared with those in 80 women in whom these techniques were employed. In expressing the results, no patients were excluded for any reason. Anatomical patency was not evaluated, and functional patency was measured by conception (abortion, tubal, or term pregnancy). Of the 80 women who had tuboplasty with conventional surgery, 10 (12.5%) successfully delivered at term, but 14 (17%) had tubal gestations. Almost 28% of the women (22) had successful pregnancies after microsurgery, but in 9 (11%) extrauterine pregnancies resulted. The three women with current intrauterine pregnancies could improve the microsurgical series to 31% successful pregnancies. Our initial experience with microsurgical techniques seems to show an increased number of term pregnancies, principally in patients who have undergone anastomosis.", "contents": "An analysis of macrosurgical and microsurgical techniques in the management of the tuboperitoneal factor in infertility. One hundred and sixty tubal reconstructions were analyzed according to a classification which describes the type of tubal repair and the tubal segment treated. The results in 80 women who were operated upon just prior to the application of microsurgical principles were compared with those in 80 women in whom these techniques were employed. In expressing the results, no patients were excluded for any reason. Anatomical patency was not evaluated, and functional patency was measured by conception (abortion, tubal, or term pregnancy). Of the 80 women who had tuboplasty with conventional surgery, 10 (12.5%) successfully delivered at term, but 14 (17%) had tubal gestations. Almost 28% of the women (22) had successful pregnancies after microsurgery, but in 9 (11%) extrauterine pregnancies resulted. The three women with current intrauterine pregnancies could improve the microsurgical series to 31% successful pregnancies. Our initial experience with microsurgical techniques seems to show an increased number of term pregnancies, principally in patients who have undergone anastomosis."}
{"id": "PMID:488423", "title": "Etiologies and subsequent reproductive performance of 100 couples with recurrent abortion.", "content": "From 1958 through 1977, 100 couples with documented recurrent reproductive failure were evaluated in the reproductive endocrine unit of the Medical College of Georgia. All couples underwent cytogenetic studies, radiologic evaluation of the M\u00fcllerian system, and timed endometrial biopsy. Recurrent pregnancy wastage was found to be associated with genetic disorders in 25 couples. M\u00fcllerian anomalies in 15, endocrine anomalies in 23, and negative findings in 37. The subsequent reproductive performance of each group is reviewed.", "contents": "Etiologies and subsequent reproductive performance of 100 couples with recurrent abortion. From 1958 through 1977, 100 couples with documented recurrent reproductive failure were evaluated in the reproductive endocrine unit of the Medical College of Georgia. All couples underwent cytogenetic studies, radiologic evaluation of the M\u00fcllerian system, and timed endometrial biopsy. Recurrent pregnancy wastage was found to be associated with genetic disorders in 25 couples. M\u00fcllerian anomalies in 15, endocrine anomalies in 23, and negative findings in 37. The subsequent reproductive performance of each group is reviewed."}
{"id": "PMID:488424", "title": "Corpus luteum dysfunction: serum progesterone levels in diagnosis and assessment of therapy for recurrent and threatened abortion.", "content": "These studies were designed to show that properly timed measurements of serum progesterone (P) can be conveniently used in the diagnosis and treatment of patients with recurrent and threatened abortion. Luteal phase serum P levels between 2 and 10 ng/ml and serum P levels below 15 ng/ml in the first 10 weeks of gestation were considered diagnostic of corpus luteum (CL) dysfunction. Patients were treated with clomiphene, gonadotropins, and/or progesterone suppositories in order to correct serum P levels, thus elevating the serum P into the normal range. When treatment of patients with subnormal P levels resulted in normalization of serum P, successful pregnancies occurred. CL dysfunctions, either before or after conception, were found in eight of the nine patients with histories of recurrent spontaneous abortions. Correction of serum P was associated with successful pregnancy in these eight patients. Twelve patients with threatened abortion were also found to have subnormal serum P levels. Progesterone suppositories corrected the serum P levels in nine of the eleven patients treated, and none of these patients aborted. Serum P measurements provide a means for evaluation of CL function during early gestation. Management of patients with CL dysfunction can also be monitored with serial serum P measurements, provided that progesterone is the therapeutic agent rather than synthetic progestins.", "contents": "Corpus luteum dysfunction: serum progesterone levels in diagnosis and assessment of therapy for recurrent and threatened abortion. These studies were designed to show that properly timed measurements of serum progesterone (P) can be conveniently used in the diagnosis and treatment of patients with recurrent and threatened abortion. Luteal phase serum P levels between 2 and 10 ng/ml and serum P levels below 15 ng/ml in the first 10 weeks of gestation were considered diagnostic of corpus luteum (CL) dysfunction. Patients were treated with clomiphene, gonadotropins, and/or progesterone suppositories in order to correct serum P levels, thus elevating the serum P into the normal range. When treatment of patients with subnormal P levels resulted in normalization of serum P, successful pregnancies occurred. CL dysfunctions, either before or after conception, were found in eight of the nine patients with histories of recurrent spontaneous abortions. Correction of serum P was associated with successful pregnancy in these eight patients. Twelve patients with threatened abortion were also found to have subnormal serum P levels. Progesterone suppositories corrected the serum P levels in nine of the eleven patients treated, and none of these patients aborted. Serum P measurements provide a means for evaluation of CL function during early gestation. Management of patients with CL dysfunction can also be monitored with serial serum P measurements, provided that progesterone is the therapeutic agent rather than synthetic progestins."}
{"id": "PMID:488425", "title": "A significant association between spontaneous abortion and tubal ectopic pregnancy.", "content": "A retrospective study of 83 patients with pathologically documented tubal ectopic pregnancy demonstrated a statistically significant association (P less than 0.005) between prior spontaneous abortion and ectopic pregnancy. In this population with a low incidence of chronic salpingitis, it is suggested that delayed (post-midcycle) ovulation, causing preovulatory over-ripeness of the ovum, may be the common underlying etiologic factor. The clinical significance of this finding is also briefly discussed.", "contents": "A significant association between spontaneous abortion and tubal ectopic pregnancy. A retrospective study of 83 patients with pathologically documented tubal ectopic pregnancy demonstrated a statistically significant association (P less than 0.005) between prior spontaneous abortion and ectopic pregnancy. In this population with a low incidence of chronic salpingitis, it is suggested that delayed (post-midcycle) ovulation, causing preovulatory over-ripeness of the ovum, may be the common underlying etiologic factor. The clinical significance of this finding is also briefly discussed."}
{"id": "PMID:488426", "title": "The relation between plasma testosterone levels and the lengths of phases of the menstrual cycle.", "content": "Plasma testosterone levels were measured in 331 women of reproductive age. The incidence of clinical signs of hyperandrogenism (hirsutism, acne) was recorded. Ovulatory activity was evaluated clinically by basal body temperature and frequent observation of changes in the appearance of the cervical os and cervical mucus. Plasma testosterone levels were abnormally elevated in patients with clinical signs of hyperandrogenism. The highest mean testosterone levels were noted in the group of hyperandrogenic women with amenorrhea. Significant prolongation of the follicular phase and shortening of the luteal phase were demonstrated to be associated with clinical signs of hyperandrogenism and elevated plasma testosterone levels. Statistically significant correlations between plasma testosterone levels and duration of phases of the menstrual cycle were observed. Testosterone levels were directly related to the length of the follicular phase and inversely related to the length of the luteal phase. A significant inverse correlation between the lengths of the two phases of the menstrual cycle was also demonstrated. These results suggest an association between hyperandrogenism and prolongation of the follicular phase and shortening of the luteal phase of the menstrual cycle, possibly related to the high incidence of infertility and menstrual irregularity reported for hyperandrogenic women.", "contents": "The relation between plasma testosterone levels and the lengths of phases of the menstrual cycle. Plasma testosterone levels were measured in 331 women of reproductive age. The incidence of clinical signs of hyperandrogenism (hirsutism, acne) was recorded. Ovulatory activity was evaluated clinically by basal body temperature and frequent observation of changes in the appearance of the cervical os and cervical mucus. Plasma testosterone levels were abnormally elevated in patients with clinical signs of hyperandrogenism. The highest mean testosterone levels were noted in the group of hyperandrogenic women with amenorrhea. Significant prolongation of the follicular phase and shortening of the luteal phase were demonstrated to be associated with clinical signs of hyperandrogenism and elevated plasma testosterone levels. Statistically significant correlations between plasma testosterone levels and duration of phases of the menstrual cycle were observed. Testosterone levels were directly related to the length of the follicular phase and inversely related to the length of the luteal phase. A significant inverse correlation between the lengths of the two phases of the menstrual cycle was also demonstrated. These results suggest an association between hyperandrogenism and prolongation of the follicular phase and shortening of the luteal phase of the menstrual cycle, possibly related to the high incidence of infertility and menstrual irregularity reported for hyperandrogenic women."}
{"id": "PMID:488427", "title": "Effect of prednisone on plasma testosterone levels and on duration of phases of the menstrual cycle in hyperandrogenic women.", "content": "A group of 106 women of reproductive age with laboratory and clinical evidence of hyperandrogenism was treated with prednisone. The daily dosage varied between 7.5 and 10 mg. Ovulatory activity was assessed prior to and during therapy by basal body temperature and observation of changes in the cervical os and cervical mucus. Plasma testosterone levels were significantly suppressed by prednisone therapy. This was associated with initiation of ovulatory activity in 5 of 14 (35.7%) amenorrheic patients and 10 of 11 (90.9%) anovulatory patients. In 81 ovulatory patients, prednisone therapy resulted in statistically significant shortening of the follicular phase and lengthening of the luteal phase of the menstrual cycle. The mean length of the menstrual cycle was unchanged. Significant correlations between percentage suppression of plasma testosterone and shortening of the follicular phase or lengthening of the luteal phase were observed. Suppression of plasma testosterone by prednisone was maximal after 2 months of treatment, while the effect on the phases of the menstrual cycle was progressive with duration of treatment. The effects of prednisone at daily dosages of 7.5 or 10 mg were not significantly different. These results suggest that prednisone therapy in hyperandrogenic women exerts an effect on both phases of the menstrual cycle, possibly related to suppression of plasma testosterone levels.", "contents": "Effect of prednisone on plasma testosterone levels and on duration of phases of the menstrual cycle in hyperandrogenic women. A group of 106 women of reproductive age with laboratory and clinical evidence of hyperandrogenism was treated with prednisone. The daily dosage varied between 7.5 and 10 mg. Ovulatory activity was assessed prior to and during therapy by basal body temperature and observation of changes in the cervical os and cervical mucus. Plasma testosterone levels were significantly suppressed by prednisone therapy. This was associated with initiation of ovulatory activity in 5 of 14 (35.7%) amenorrheic patients and 10 of 11 (90.9%) anovulatory patients. In 81 ovulatory patients, prednisone therapy resulted in statistically significant shortening of the follicular phase and lengthening of the luteal phase of the menstrual cycle. The mean length of the menstrual cycle was unchanged. Significant correlations between percentage suppression of plasma testosterone and shortening of the follicular phase or lengthening of the luteal phase were observed. Suppression of plasma testosterone by prednisone was maximal after 2 months of treatment, while the effect on the phases of the menstrual cycle was progressive with duration of treatment. The effects of prednisone at daily dosages of 7.5 or 10 mg were not significantly different. These results suggest that prednisone therapy in hyperandrogenic women exerts an effect on both phases of the menstrual cycle, possibly related to suppression of plasma testosterone levels."}
{"id": "PMID:488428", "title": "Early and late hormonal changes following progesterone injection to patients with secondary amenorrhea.", "content": "In humans, progesterone (Po) administration has been shown capable of eliciting luteinizing hormone (LH) and prolactin surges in a good estrogenic milieu, but whether such changes induce corpus luteum function still remains controversial. We therefore proposed to explore further the second hypothesis in a placebo-controlled type of study. In patients with secondary amenorrhea and low circulating estradiol (E2) levels with or without ovaries, a 50-mg progesterone injection induced only a short and transient rise in plasma Po levels. On the other hand, a similar injection to patients with mid-to late follicular levels of circulating E2 and intact ovaries induced a surge of plasma LH and prolactin which was followed by a sustained elevation in plasma Po. Furthermore, a rise in basal body temperature accompanied by evidence of secretory endometrium was also observed in these patients. It is concluded that, in a proper endocrine milieu, progesterone administration may induce luteinization in human subjects.", "contents": "Early and late hormonal changes following progesterone injection to patients with secondary amenorrhea. In humans, progesterone (Po) administration has been shown capable of eliciting luteinizing hormone (LH) and prolactin surges in a good estrogenic milieu, but whether such changes induce corpus luteum function still remains controversial. We therefore proposed to explore further the second hypothesis in a placebo-controlled type of study. In patients with secondary amenorrhea and low circulating estradiol (E2) levels with or without ovaries, a 50-mg progesterone injection induced only a short and transient rise in plasma Po levels. On the other hand, a similar injection to patients with mid-to late follicular levels of circulating E2 and intact ovaries induced a surge of plasma LH and prolactin which was followed by a sustained elevation in plasma Po. Furthermore, a rise in basal body temperature accompanied by evidence of secretory endometrium was also observed in these patients. It is concluded that, in a proper endocrine milieu, progesterone administration may induce luteinization in human subjects."}
{"id": "PMID:488429", "title": "A simple and sensitive nonradioactive method for the detection of urinary human chorionic gonadotropin and diagnosis of early human pregnancy. I. Multiple-unit test.", "content": "A simple, sensitive, and reproducible method for the detection of urinary human chorionic gonadotropin (hCG) and diagnosis of early human pregnancy is reported. A 5-ml aliquot of filtered early-morning urine sample was concentrated in a microconcentrator (M) to 0.1 ml of retentate which was diluted with 0.4 ml of distilled water and tested in a hemagglutination inhibition test (M-HIT). Also, a 0.1-ml aliquot of filtered unconcentrated urine sample was diluted with 0.4 ml of distilled water and tested in the same hemagglutination inhibition test (HIT). Urine samples from women of reproductive age; from perimenopausal, menopausal, and proteinuric women; and from adult males were tested in the HIT and M-HIT. Some of these urine samples were also tested in the mouse ovulation bioassay (MOB). The M-HIT was significantly more reliable than the HIT for diagnosis of early pregnancy 25 to 55 days after menses. Correct negative results with the M-HIT were obtained in urine samples of most of the nonpregnant cycling, perimenopausal, and menopausal women, and adult males. Urine samples from subjects with severe proteinuria gave false-positive types of reactions in the M-HIT. Positive results were obtained in the MOB with a number of urine samples from pregnant, perimenopausal, and menopausal women. A properly conducted M-HIT should be very valuable in diagnosing pregnancy as early as the 26th day of the cycle in regularly menstruating women.", "contents": "A simple and sensitive nonradioactive method for the detection of urinary human chorionic gonadotropin and diagnosis of early human pregnancy. I. Multiple-unit test. A simple, sensitive, and reproducible method for the detection of urinary human chorionic gonadotropin (hCG) and diagnosis of early human pregnancy is reported. A 5-ml aliquot of filtered early-morning urine sample was concentrated in a microconcentrator (M) to 0.1 ml of retentate which was diluted with 0.4 ml of distilled water and tested in a hemagglutination inhibition test (M-HIT). Also, a 0.1-ml aliquot of filtered unconcentrated urine sample was diluted with 0.4 ml of distilled water and tested in the same hemagglutination inhibition test (HIT). Urine samples from women of reproductive age; from perimenopausal, menopausal, and proteinuric women; and from adult males were tested in the HIT and M-HIT. Some of these urine samples were also tested in the mouse ovulation bioassay (MOB). The M-HIT was significantly more reliable than the HIT for diagnosis of early pregnancy 25 to 55 days after menses. Correct negative results with the M-HIT were obtained in urine samples of most of the nonpregnant cycling, perimenopausal, and menopausal women, and adult males. Urine samples from subjects with severe proteinuria gave false-positive types of reactions in the M-HIT. Positive results were obtained in the MOB with a number of urine samples from pregnant, perimenopausal, and menopausal women. A properly conducted M-HIT should be very valuable in diagnosing pregnancy as early as the 26th day of the cycle in regularly menstruating women."}
{"id": "PMID:488430", "title": "A simple and sensitive nonradioactive method for the detection of urinary human chorionic gonadotropin and diagnosis of early human pregnancy. II. Single-unit test.", "content": "A simple, sensitive, and reliable single-unit nonradioactive method for the detection of human chorionic gonadotropin (hCG) in concentrated urine and the diagnosis of early pregnancy is reported. This unit, presently termed the Ayerst pregnancy test kit (APTK), consists of four components: a sampler-filter paper cone, an ultrafilter-concentrator to which a vial holder is attached, a support stand with a mirror, and an immunologic reagent vial. In the APTK, 5 to 6 ml of urine were sampled, filtered, and concentrated, and the hCG in the retentate was detected by Ayerst immunologic reagents [APTK(AY)] and by the Pregnosticon \"All In\" [APTK(P)]. Some of the unconcentrated urine samples (0.1 ml) were also tested in hemagglutination inhibition tests (HIT) using Ayerst [HIG(AY)] and Pregnosticon \"All In\" [HIT(P)] reagents. Urine samples from pregnant, nonpregnant (ovulating and nonovulating), perimenopausal, and menopausal women were tested. It was found that the APTK(AY) and APTK(P) were significantly more sensitive and reliable than the HIT(AY) and HIT(P) in detecting low levels of urinary hCG for early diagnosis of pregnancy. The sensitivity and specificity of the APTK(AY) were better than those of the APTK(P). The APTK(AY) give significantly more correct positive and negative results than the other tests performed simultaneously. The APTK(AY) is simpler and safer than the serum radioimmunoassays and radioreceptor assay presently used to detect low levels of hCG for the early diagnosis of pregnancy and other hCG-producing states.", "contents": "A simple and sensitive nonradioactive method for the detection of urinary human chorionic gonadotropin and diagnosis of early human pregnancy. II. Single-unit test. A simple, sensitive, and reliable single-unit nonradioactive method for the detection of human chorionic gonadotropin (hCG) in concentrated urine and the diagnosis of early pregnancy is reported. This unit, presently termed the Ayerst pregnancy test kit (APTK), consists of four components: a sampler-filter paper cone, an ultrafilter-concentrator to which a vial holder is attached, a support stand with a mirror, and an immunologic reagent vial. In the APTK, 5 to 6 ml of urine were sampled, filtered, and concentrated, and the hCG in the retentate was detected by Ayerst immunologic reagents [APTK(AY)] and by the Pregnosticon \"All In\" [APTK(P)]. Some of the unconcentrated urine samples (0.1 ml) were also tested in hemagglutination inhibition tests (HIT) using Ayerst [HIG(AY)] and Pregnosticon \"All In\" [HIT(P)] reagents. Urine samples from pregnant, nonpregnant (ovulating and nonovulating), perimenopausal, and menopausal women were tested. It was found that the APTK(AY) and APTK(P) were significantly more sensitive and reliable than the HIT(AY) and HIT(P) in detecting low levels of urinary hCG for early diagnosis of pregnancy. The sensitivity and specificity of the APTK(AY) were better than those of the APTK(P). The APTK(AY) give significantly more correct positive and negative results than the other tests performed simultaneously. The APTK(AY) is simpler and safer than the serum radioimmunoassays and radioreceptor assay presently used to detect low levels of hCG for the early diagnosis of pregnancy and other hCG-producing states."}
{"id": "PMID:488431", "title": "Relationship between plasma prolactin and the endocrine microenvironment of the developing human antral follicle.", "content": "The aim of the present study was to determine whether any obvious relationships exist between the circulating levels of prolactin at the time of ovariectomy and the endocrine microenvironment and developmental status of antral follicles. The concentrations of prolactin, follicle-stimulating hormone (FSH), and estradiol were measured in peripheral plasma and in antral fluid of follicles collected at varying stages of the menstrual cycle. In addition, the granulosa cells were recovered from each follicle (greater than or equal to 4 mm in diameter) and their numbers were quantitated. When the plasma levels of prolactin ranged from 11 to 100 ng/ml, the antral fluid levels of prolactin were uniformly low (less than 20 ng/ml) and the over-all level of intrafollicular activity remained unchanged. However, when the prolactin concentrations in plasma exceeded 100 ng/ml, the levels of prolactin in antral fluid were significantly elevated. Moreover, the high levels of intrafollicular prolactin were associated with a marked reduction in FSH accumulation and low levels of estradiol in antral fluid. Also, these follicles were severely deficient in granulosa cells. This marked reduction in intrafollicular activity was not associated with any significant changes in the mean levels of estradiol and FSH in peripheral plasma. These findings suggest that hyperprolactinemia is associated with a marked reduction in intraovarian activity and that the extent of this reduction may not be always apparent from the levels of circulating estradiol.", "contents": "Relationship between plasma prolactin and the endocrine microenvironment of the developing human antral follicle. The aim of the present study was to determine whether any obvious relationships exist between the circulating levels of prolactin at the time of ovariectomy and the endocrine microenvironment and developmental status of antral follicles. The concentrations of prolactin, follicle-stimulating hormone (FSH), and estradiol were measured in peripheral plasma and in antral fluid of follicles collected at varying stages of the menstrual cycle. In addition, the granulosa cells were recovered from each follicle (greater than or equal to 4 mm in diameter) and their numbers were quantitated. When the plasma levels of prolactin ranged from 11 to 100 ng/ml, the antral fluid levels of prolactin were uniformly low (less than 20 ng/ml) and the over-all level of intrafollicular activity remained unchanged. However, when the prolactin concentrations in plasma exceeded 100 ng/ml, the levels of prolactin in antral fluid were significantly elevated. Moreover, the high levels of intrafollicular prolactin were associated with a marked reduction in FSH accumulation and low levels of estradiol in antral fluid. Also, these follicles were severely deficient in granulosa cells. This marked reduction in intrafollicular activity was not associated with any significant changes in the mean levels of estradiol and FSH in peripheral plasma. These findings suggest that hyperprolactinemia is associated with a marked reduction in intraovarian activity and that the extent of this reduction may not be always apparent from the levels of circulating estradiol."}
{"id": "PMID:488432", "title": "Tissue testosterone and dihydrotestosterone from bilateral testis biopsies in males with varicocele.", "content": "Nine males with varying degrees of subfertility with left varicocele underwent bilateral testicular biopsy for determination of testicular testosterone (T) and dihydrotestosterone (DHT) content. Testicular tissue T and DHT levels varied greatly, and there was no significant difference when the group T and DHT content of the left testis was compared with that of the right testis. When the respective mean preoperative sperm count was compared with the bilateral tissue T and DHT content, no consistent relationship was found.", "contents": "Tissue testosterone and dihydrotestosterone from bilateral testis biopsies in males with varicocele. Nine males with varying degrees of subfertility with left varicocele underwent bilateral testicular biopsy for determination of testicular testosterone (T) and dihydrotestosterone (DHT) content. Testicular tissue T and DHT levels varied greatly, and there was no significant difference when the group T and DHT content of the left testis was compared with that of the right testis. When the respective mean preoperative sperm count was compared with the bilateral tissue T and DHT content, no consistent relationship was found."}
{"id": "PMID:488433", "title": "Factors affecting sperm motility. II. Human sperm velocity and percentage of motility as influenced by semen dilution.", "content": "Spermatozoal velocity and percentage of motility were analyzed objectively with the multiple exposure photography method before and after specimens from fertile and infertile men were diluted in their own seminal plasma or normal saline. No significant change in percentage of motility was found in samples diluted up to 1:6 in both kinds of diluents. However, a significant relative increase (up to 25% of the original velocity) was found when a specimen was diluted with its own seminal plasma, and an even greater increase (up to 37% of the original velocity) was found when it was diluted with saline. Compared with undiluted specimens, there was no delayed effect on spermatozoal motility when semen was diluted with saline after up to 4 hours' incubation time. Contrary to the findings in animal and human semen described by others, there was no deleterious effect on sperm motility with this kind and rate of dilution and duration of time. The assumption that the increase in sperm velocity caused by dilution is not excitatory but is due only to a decrease of seminal fluid viscosity and a reduced number of spermatozoa which interfere with sperm free movement is discussed. We recommend evaluation of spermatozoal motility in diluted specimens in addition to evaluation of the original specimen in any routine semen analysis in order to determine true spermatozoal motility potential under optimal conditions.", "contents": "Factors affecting sperm motility. II. Human sperm velocity and percentage of motility as influenced by semen dilution. Spermatozoal velocity and percentage of motility were analyzed objectively with the multiple exposure photography method before and after specimens from fertile and infertile men were diluted in their own seminal plasma or normal saline. No significant change in percentage of motility was found in samples diluted up to 1:6 in both kinds of diluents. However, a significant relative increase (up to 25% of the original velocity) was found when a specimen was diluted with its own seminal plasma, and an even greater increase (up to 37% of the original velocity) was found when it was diluted with saline. Compared with undiluted specimens, there was no delayed effect on spermatozoal motility when semen was diluted with saline after up to 4 hours' incubation time. Contrary to the findings in animal and human semen described by others, there was no deleterious effect on sperm motility with this kind and rate of dilution and duration of time. The assumption that the increase in sperm velocity caused by dilution is not excitatory but is due only to a decrease of seminal fluid viscosity and a reduced number of spermatozoa which interfere with sperm free movement is discussed. We recommend evaluation of spermatozoal motility in diluted specimens in addition to evaluation of the original specimen in any routine semen analysis in order to determine true spermatozoal motility potential under optimal conditions."}
{"id": "PMID:488434", "title": "Accumulation, nature, and possible functions of the malachite green affinity material in ejaculated human spermatozoa.", "content": "An electron microscopic study was conducted on human sperm over an incubation period of 5 to 6 hours in Tyrode's solution at room temperature. Examination of aliquots of the cells, fixed at timed intervals, with glutaraldehyde, malachite green, and postosmication revealed that malachite green affinity material (MGA-M) was barely discernible at first but did accumulate considerably upon standing. Biochemical analysis of MGA-M, which is extractable by glutaraldehyde, revealed that MGA-M is a mixture of extractable phospholipids and of their lyso-derivatives. Some of these substances have fusogenic properties; i.e., they are able to fuse together the membranes of two different cells. The appearance and accumulation of these fusogens occurred during the incubation period of 5 to 6 hours, which was previously shown to be required to capacitate human sperm in vitro. It is probable, therefore, that human sperm, during their initial period of incubation either in vivo or in vitro, not only become capacitated and undergo the acrosome reaction but also develop the fusogenic substance(s) which are necessary for the imminent fusion of their plasma membrane to the vitelline membrane of the mature oocyte.", "contents": "Accumulation, nature, and possible functions of the malachite green affinity material in ejaculated human spermatozoa. An electron microscopic study was conducted on human sperm over an incubation period of 5 to 6 hours in Tyrode's solution at room temperature. Examination of aliquots of the cells, fixed at timed intervals, with glutaraldehyde, malachite green, and postosmication revealed that malachite green affinity material (MGA-M) was barely discernible at first but did accumulate considerably upon standing. Biochemical analysis of MGA-M, which is extractable by glutaraldehyde, revealed that MGA-M is a mixture of extractable phospholipids and of their lyso-derivatives. Some of these substances have fusogenic properties; i.e., they are able to fuse together the membranes of two different cells. The appearance and accumulation of these fusogens occurred during the incubation period of 5 to 6 hours, which was previously shown to be required to capacitate human sperm in vitro. It is probable, therefore, that human sperm, during their initial period of incubation either in vivo or in vitro, not only become capacitated and undergo the acrosome reaction but also develop the fusogenic substance(s) which are necessary for the imminent fusion of their plasma membrane to the vitelline membrane of the mature oocyte."}
{"id": "PMID:488435", "title": "Apparent effect of ascorbic acid medication on semen metal levels.", "content": "The apparent effect of ascorbic acid therapy for nonspecific spermagglutination on semen levels of ascorbic acid as well as macro- and micrometals was determined in 20 men (ages 25 to 38). Pretreatment diagnosis was based on infertility and relatively low ratings in sperm density, motility, motility index, and semen volume, and were associated with large numbers of abnormal sperm, sperm precursors, and leukocytes. The pretreatment levels of ascorbic acid, sodium, iron, potassium, zinc, manganese, lead, magnesium, and copper were measured in each patient's semen and compared with levels following 60 days of dietary vitamin C supplementation (1.0 gm/day). Analysis of the vitamin C preparation prescribed revealed that each subject was given an impure ascorbic acid medication to supplement a normal diet. Therefore, the significant increases in levels of ascorbic acid and metals in semen following therapy could not be attributed to ascorbic acid alone, nor, similarly, the improved physical parameters of each subject's semen following therapy; no apparent spermagglutination and restored fertility may be due to the interaction of ascorbic acid with cations found in semen.", "contents": "Apparent effect of ascorbic acid medication on semen metal levels. The apparent effect of ascorbic acid therapy for nonspecific spermagglutination on semen levels of ascorbic acid as well as macro- and micrometals was determined in 20 men (ages 25 to 38). Pretreatment diagnosis was based on infertility and relatively low ratings in sperm density, motility, motility index, and semen volume, and were associated with large numbers of abnormal sperm, sperm precursors, and leukocytes. The pretreatment levels of ascorbic acid, sodium, iron, potassium, zinc, manganese, lead, magnesium, and copper were measured in each patient's semen and compared with levels following 60 days of dietary vitamin C supplementation (1.0 gm/day). Analysis of the vitamin C preparation prescribed revealed that each subject was given an impure ascorbic acid medication to supplement a normal diet. Therefore, the significant increases in levels of ascorbic acid and metals in semen following therapy could not be attributed to ascorbic acid alone, nor, similarly, the improved physical parameters of each subject's semen following therapy; no apparent spermagglutination and restored fertility may be due to the interaction of ascorbic acid with cations found in semen."}
{"id": "PMID:488436", "title": "Viability of spermatozoa in the human ejaculate after vasectomy.", "content": "Ejaculates produced by 82 men between 6 and 19 days after removal of approximately 5 cm of vas deferens were assessed specifically for motility of residual spermatozoa. Several of 23 ejaculates produced 6 to 8 days after vasectomy still contained spermatozoa of the quality and number likely to produce pregnancy. By 13 to 15 days, however, all or the great majority of residual spermatozoa were dead, and in only 1 of 50 men were there numbers of motile spermatozoa possibly compatible with fertility. The results seem to justify more extensive surveys to confirm the absolute or biologic limits of the viability of spermatozoa in the terminal portion of the human male reproductive tract, anticipated here to be 16 to 18 days, as a suggested basis for a standard postvasectomy protocol. Second, these data support the choice of the 3rd postoperative week as the earliest rational moment to confirm the absence of motile spermatozoa, or in their presence to suspect an incomplete block.", "contents": "Viability of spermatozoa in the human ejaculate after vasectomy. Ejaculates produced by 82 men between 6 and 19 days after removal of approximately 5 cm of vas deferens were assessed specifically for motility of residual spermatozoa. Several of 23 ejaculates produced 6 to 8 days after vasectomy still contained spermatozoa of the quality and number likely to produce pregnancy. By 13 to 15 days, however, all or the great majority of residual spermatozoa were dead, and in only 1 of 50 men were there numbers of motile spermatozoa possibly compatible with fertility. The results seem to justify more extensive surveys to confirm the absolute or biologic limits of the viability of spermatozoa in the terminal portion of the human male reproductive tract, anticipated here to be 16 to 18 days, as a suggested basis for a standard postvasectomy protocol. Second, these data support the choice of the 3rd postoperative week as the earliest rational moment to confirm the absence of motile spermatozoa, or in their presence to suspect an incomplete block."}
{"id": "PMID:488437", "title": "Active immunization of female rabbits with purified rabbit acrosin and effect on fertility.", "content": "Acrosin immunogen was purified from rabbit testes by sequential acid extraction, ammonium sulfate fractionation, cation-exchange, and affinity chromatography. Twelve females received intradermal injections of purified acrosin in Freund's complete adjuvant followed by a booster injection 6 weeks later. A radioimmunoassay for rabbit acrosin was developed and used to monitor the immune response of the recipients. The females were mated at the time when serum titers of acrosin antibodies were maximal. Four of the animals did not become pregnant, and three of these had the highest antibody titers in the total group. The remaining eight rabbits delivered normal litters at term. Of four control females (immunized with bovine serum albumin), one did not become pregnant. The pregnancy rates for the control and acrosin-immunized rabbits were 75% and 67%, respectively. It is concluded that, although active immunization with acrosin had no significant effect on fertility, the antibody titer produced may be a factor.", "contents": "Active immunization of female rabbits with purified rabbit acrosin and effect on fertility. Acrosin immunogen was purified from rabbit testes by sequential acid extraction, ammonium sulfate fractionation, cation-exchange, and affinity chromatography. Twelve females received intradermal injections of purified acrosin in Freund's complete adjuvant followed by a booster injection 6 weeks later. A radioimmunoassay for rabbit acrosin was developed and used to monitor the immune response of the recipients. The females were mated at the time when serum titers of acrosin antibodies were maximal. Four of the animals did not become pregnant, and three of these had the highest antibody titers in the total group. The remaining eight rabbits delivered normal litters at term. Of four control females (immunized with bovine serum albumin), one did not become pregnant. The pregnancy rates for the control and acrosin-immunized rabbits were 75% and 67%, respectively. It is concluded that, although active immunization with acrosin had no significant effect on fertility, the antibody titer produced may be a factor."}
{"id": "PMID:488442", "title": "[Effect of the contraction of other muscle groups on muscle strength, latency period and endurance].", "content": "The following investigation was undertaken in connection with Sechenov's idea on mutual influence of muscles performing different work. It deals with the influence on test muscle (TM) efficiency of other simultaneously working muscles (SWM). It was shown that tension of SWM or maintenance of complex postures exert a negative influence on the force and latency of the visual-motor reaction and the static effort endurance. The mechanism involved is external inhibition. After its extinction as a result of repeated combinations of both activities the degree of lowering of TM activity grows less and finally its improvement is observed as compared to data obtained in the absence of SWM contractions. It is suggested that when external inhibition is extinguished impulses arising in SWM reinforce and improve according to the law of dominant the functions of nervous centers regulating activities of the TM.", "contents": "[Effect of the contraction of other muscle groups on muscle strength, latency period and endurance]. The following investigation was undertaken in connection with Sechenov's idea on mutual influence of muscles performing different work. It deals with the influence on test muscle (TM) efficiency of other simultaneously working muscles (SWM). It was shown that tension of SWM or maintenance of complex postures exert a negative influence on the force and latency of the visual-motor reaction and the static effort endurance. The mechanism involved is external inhibition. After its extinction as a result of repeated combinations of both activities the degree of lowering of TM activity grows less and finally its improvement is observed as compared to data obtained in the absence of SWM contractions. It is suggested that when external inhibition is extinguished impulses arising in SWM reinforce and improve according to the law of dominant the functions of nervous centers regulating activities of the TM."}
{"id": "PMID:488443", "title": "[\"Inhibition of inhibition\" in neurons of the cerebral cortex].", "content": "Light flashes and single electrical stimuli applied to the nucleus ventro-posterolateralis and to the LGB evoked protracted IPSPs in visual and sensorimotor cortical neurons respectively. High-frequency stimulation of the midbrain RF suppressed these IPSPs which was manifested in a shortening of the inhibitory pause and in a decrease in the amplitude of the IPSPs up to their complete elimination (disinhibition). Activation of noradrenergic pathways by stimuli applied to the locus coeruleus or to the lateral hypothalamus led to the same result. The suppression of cortical IPSPs followed by facilitation of cortical unit firing in response to thalamic or peripheral stimulation seems to be an imprortant mechanism underlying arousal.", "contents": "[\"Inhibition of inhibition\" in neurons of the cerebral cortex]. Light flashes and single electrical stimuli applied to the nucleus ventro-posterolateralis and to the LGB evoked protracted IPSPs in visual and sensorimotor cortical neurons respectively. High-frequency stimulation of the midbrain RF suppressed these IPSPs which was manifested in a shortening of the inhibitory pause and in a decrease in the amplitude of the IPSPs up to their complete elimination (disinhibition). Activation of noradrenergic pathways by stimuli applied to the locus coeruleus or to the lateral hypothalamus led to the same result. The suppression of cortical IPSPs followed by facilitation of cortical unit firing in response to thalamic or peripheral stimulation seems to be an imprortant mechanism underlying arousal."}
{"id": "PMID:488444", "title": "[Bilateral motor cortex effects on interneurons of the ventral horn of the lumbar segments].", "content": "The ventral horn interneurons in the spinal cord L6-7 segments were recorded in anesthetized cats during stimulation of the pericruciate cortex of both cerebral hemispheres. The majority of ventral interneurons are activated by volleys from the contralateral motor cortex and nearly half of them--by volleys from the ipsilateral cortex as well. Two kinds of firing patterns occurred: separate spikes and bursts of spikes. The latency of the 1st type firing was significantly greater than that of the 2nd one. There was a close relation of convergence of the afferent and corticospinal volleys on ventral horn interneurons: neurons activated by ipsilateral afferents only received synaptic influence only from the contralateral motor cortex whereas neurons with ipsilateral as well as contralateral inputs were activated by corticospinal volleys from both hemispheres. The interneurons with bilateral segmental and supraspinal connections seem to be involved in integrative action as important elements of the neuronal mechanism of bilateral motor coordination.", "contents": "[Bilateral motor cortex effects on interneurons of the ventral horn of the lumbar segments]. The ventral horn interneurons in the spinal cord L6-7 segments were recorded in anesthetized cats during stimulation of the pericruciate cortex of both cerebral hemispheres. The majority of ventral interneurons are activated by volleys from the contralateral motor cortex and nearly half of them--by volleys from the ipsilateral cortex as well. Two kinds of firing patterns occurred: separate spikes and bursts of spikes. The latency of the 1st type firing was significantly greater than that of the 2nd one. There was a close relation of convergence of the afferent and corticospinal volleys on ventral horn interneurons: neurons activated by ipsilateral afferents only received synaptic influence only from the contralateral motor cortex whereas neurons with ipsilateral as well as contralateral inputs were activated by corticospinal volleys from both hemispheres. The interneurons with bilateral segmental and supraspinal connections seem to be involved in integrative action as important elements of the neuronal mechanism of bilateral motor coordination."}
{"id": "PMID:488445", "title": "[Correlation between nonspecific and specific excitation in the cat somatosensory cortex during different modalities of stimulation].", "content": "Multiunit activity and EEG recorded with the same electrode from the somatosensory cortex in unrestrained cats, were compared. The obviously identical patterns of the EEG activation coincided with different multiunit patterns (acceleration or deceleration of frequency, modification of discharge order) depending on the stimulus modality (somatic, acoustic, direct RF stimulation) and on the foregoing background impulsation. This suggests that the EEG activation, although being basically unspecific, has specific features. The sensory information, apart from the known forms, seems to be coded in the form of diverse modes of functional activity of a neuronal population localized in a brain structure.", "contents": "[Correlation between nonspecific and specific excitation in the cat somatosensory cortex during different modalities of stimulation]. Multiunit activity and EEG recorded with the same electrode from the somatosensory cortex in unrestrained cats, were compared. The obviously identical patterns of the EEG activation coincided with different multiunit patterns (acceleration or deceleration of frequency, modification of discharge order) depending on the stimulus modality (somatic, acoustic, direct RF stimulation) and on the foregoing background impulsation. This suggests that the EEG activation, although being basically unspecific, has specific features. The sensory information, apart from the known forms, seems to be coded in the form of diverse modes of functional activity of a neuronal population localized in a brain structure."}
{"id": "PMID:488446", "title": "[Motor components of \"caudate arrest\"].", "content": "In chronic experiments on 4 defense-conditioned dogs maintaining a certain posture, the analysis of motor components of the \"caudate arrest\" (induced by high-frequency stimulation of the caudate nucleus) and their comparison with the characteristics of the myographic and motor components of normal cessation of the legs (i. e. in absence of the conditioning signal), showed the \"caudate arrest\" not to be a model for arrest of a voluntary movement. The caudate nucleus seems not to be involved structurally in the instrumental defense response but to belong to a parallel system controlling all stages of the response.", "contents": "[Motor components of \"caudate arrest\"]. In chronic experiments on 4 defense-conditioned dogs maintaining a certain posture, the analysis of motor components of the \"caudate arrest\" (induced by high-frequency stimulation of the caudate nucleus) and their comparison with the characteristics of the myographic and motor components of normal cessation of the legs (i. e. in absence of the conditioning signal), showed the \"caudate arrest\" not to be a model for arrest of a voluntary movement. The caudate nucleus seems not to be involved structurally in the instrumental defense response but to belong to a parallel system controlling all stages of the response."}
{"id": "PMID:488448", "title": "[Activity of several the mediator systems of the brain during hyperbaric oxygenation].", "content": "Dynamics of noradrenalin, adrenalin, dipeptide homocarnosine, GABA, hystidine, MAO activity (with noradrenalin as substrate), and homocarnosincarnosinsynthetase in the rat brain under hyperbaroxygenation, was studied. Exposure of the rats to 4 atm of oxygen for 1 hr leads to the 70--80% decrease of noradrenalin content in all brain areas. Adrenalin level does not change. MAO activity increases in hypothalamus remaining at the control level in cortex, decreases significantly in medulla oblongata, and complete inhibition of the enzyme activity occurs in the midbrain. The hyperbaric conditions cause no considerable changes in homocarnosine, GABA, and hystidine levels in all the areas under study. A profound decrease of homocarnosine content and inhibition of homocarnosine--carnosinesynthetase follows the convulsive phase of oxygen intoxication especially in the midbrain (69.9% and 50.3%, resp.).", "contents": "[Activity of several the mediator systems of the brain during hyperbaric oxygenation]. Dynamics of noradrenalin, adrenalin, dipeptide homocarnosine, GABA, hystidine, MAO activity (with noradrenalin as substrate), and homocarnosincarnosinsynthetase in the rat brain under hyperbaroxygenation, was studied. Exposure of the rats to 4 atm of oxygen for 1 hr leads to the 70--80% decrease of noradrenalin content in all brain areas. Adrenalin level does not change. MAO activity increases in hypothalamus remaining at the control level in cortex, decreases significantly in medulla oblongata, and complete inhibition of the enzyme activity occurs in the midbrain. The hyperbaric conditions cause no considerable changes in homocarnosine, GABA, and hystidine levels in all the areas under study. A profound decrease of homocarnosine content and inhibition of homocarnosine--carnosinesynthetase follows the convulsive phase of oxygen intoxication especially in the midbrain (69.9% and 50.3%, resp.)."}
{"id": "PMID:488449", "title": "[Mediator liberation at the neuromuscular junctions of rhythmically stimulated frog sartorius muscles].", "content": "Synapses of large fibers seem to have a more obvious initial increase followed by a greater decrease in quantum content (m) of EPPs than the small fibers. The total amount of neurotransmitter released during EPPs reproduction was much greater in the large fibres. The calculation of binomial parameters of transmitter release showed that n parameter repeated the dynamics of m, while the P parameter gradually increased. The initial uptake of P and increase of n was more obvious in verve endings of the large muscle fibres. The findings suggest an unequal length of synaptic contact.", "contents": "[Mediator liberation at the neuromuscular junctions of rhythmically stimulated frog sartorius muscles]. Synapses of large fibers seem to have a more obvious initial increase followed by a greater decrease in quantum content (m) of EPPs than the small fibers. The total amount of neurotransmitter released during EPPs reproduction was much greater in the large fibres. The calculation of binomial parameters of transmitter release showed that n parameter repeated the dynamics of m, while the P parameter gradually increased. The initial uptake of P and increase of n was more obvious in verve endings of the large muscle fibres. The findings suggest an unequal length of synaptic contact."}
{"id": "PMID:488451", "title": "[Convergence of corticofugal impulse projections onto superior colliculus neurons].", "content": "In unanesthetized immobilized cats, 82.75% of tectal neurons responded to direct electrical stimulation of different cortical areas. Stimulation of the associative and visual cortex was the most effective. On simultaneous presentation of light and electrical stimuli, facilitation of responses of the collicular neurons was prevalent. The role of striatal and extrastriatal effects of neocortex as well as the regularities of integration of retinotectal and corticofugal afferents upon the neurons of anterior colliculus, are discussed.", "contents": "[Convergence of corticofugal impulse projections onto superior colliculus neurons]. In unanesthetized immobilized cats, 82.75% of tectal neurons responded to direct electrical stimulation of different cortical areas. Stimulation of the associative and visual cortex was the most effective. On simultaneous presentation of light and electrical stimuli, facilitation of responses of the collicular neurons was prevalent. The role of striatal and extrastriatal effects of neocortex as well as the regularities of integration of retinotectal and corticofugal afferents upon the neurons of anterior colliculus, are discussed."}
{"id": "PMID:488453", "title": "[Influence of the sympathetic nervous system on the electrical activity of the neuronally isolated cerebral cortex].", "content": "Bilateral transection of the pregangiolinic sympathetic cervical fibers and electrical stimulation of ipsilateral cranial and of the cervical sympathetic nerve originated reactions differing in strength and latency in intact hemisphere and in neuronally isolated neocortex, but having common origin mainly due to changes in the capacities of the delta--and theta--potentials. The direct nervous influences on electrical activity of the neuronally isolated neocortex through sympathetic fibers passing along the blood vessels, seems to be insufficient.", "contents": "[Influence of the sympathetic nervous system on the electrical activity of the neuronally isolated cerebral cortex]. Bilateral transection of the pregangiolinic sympathetic cervical fibers and electrical stimulation of ipsilateral cranial and of the cervical sympathetic nerve originated reactions differing in strength and latency in intact hemisphere and in neuronally isolated neocortex, but having common origin mainly due to changes in the capacities of the delta--and theta--potentials. The direct nervous influences on electrical activity of the neuronally isolated neocortex through sympathetic fibers passing along the blood vessels, seems to be insufficient."}
{"id": "PMID:488455", "title": "[Effect of inhibitors of the active transport of ions on the spontaneous and evoked electrical activity of parenchymal mammary gland receptors].", "content": "In the potassium-free saline spontaneous activity of the mammary gland parenchyma receptor increased within first 30--40 min., then decreased and, finally, ceased. Sensitivity of the receptor to the mechanical stimuli decreased gradually. After washing in normal saline the evoked and spontaneous activities recovered. Ouabain (5 . 10(-5)--10(-4) g/ml) exerted an effect similar to that of the potassium--free solution except that the impulse activity could not be restored by the washing in physiological saline.", "contents": "[Effect of inhibitors of the active transport of ions on the spontaneous and evoked electrical activity of parenchymal mammary gland receptors]. In the potassium-free saline spontaneous activity of the mammary gland parenchyma receptor increased within first 30--40 min., then decreased and, finally, ceased. Sensitivity of the receptor to the mechanical stimuli decreased gradually. After washing in normal saline the evoked and spontaneous activities recovered. Ouabain (5 . 10(-5)--10(-4) g/ml) exerted an effect similar to that of the potassium--free solution except that the impulse activity could not be restored by the washing in physiological saline."}
{"id": "PMID:488456", "title": "[Arterial pressure responses to stretch and contraction of the gastrocnemius during stepwise interruption of its connections to the body].", "content": "In anesthetized cats, the pressoreaction of arterial pressure to stretch with weight of 3--4 kg and to contraction of the m. gastrocnemius due to direct 6--10--threshold electrical stimulation 50/sec, occurred due to reflex from the muscle receptors in 92--93%. 80--82% of the reaction was due to the afferents of the ischiatic nerve, and 11--12% to the afferents of vascular plexus. 7--8% were due to the blood outflow from the muscle and was unrelated to the stimulation.", "contents": "[Arterial pressure responses to stretch and contraction of the gastrocnemius during stepwise interruption of its connections to the body]. In anesthetized cats, the pressoreaction of arterial pressure to stretch with weight of 3--4 kg and to contraction of the m. gastrocnemius due to direct 6--10--threshold electrical stimulation 50/sec, occurred due to reflex from the muscle receptors in 92--93%. 80--82% of the reaction was due to the afferents of the ischiatic nerve, and 11--12% to the afferents of vascular plexus. 7--8% were due to the blood outflow from the muscle and was unrelated to the stimulation."}
{"id": "PMID:488457", "title": "[Role of adreno- and cholinoreactive structures of the hypothalamus in regulating blood coagulation and maintaining it in a fluid state].", "content": "Electrical stimulation of anterior and lateral hypothalamus as well as acetylcholin injection into the same areas activated the anticoagulating blood system whereas adrenaline injections activated the coagulating system. The electrical stimulation with certain parameters seems to involve cholinoreactive structures of the hypothalamus, shifting the blood potential towards hypocoagulation. The sitmulation of the paraventricular and ventromedial hypothalamic nuclei with the same current activated the blood coagulating system whereas stimulation of the preoptic lateral or medial areas, medial supraoptic nucleus of the hypothalamus induced no changes in the homeostatic system. The data obtained suggest that the regulation of the blood anticoagulating system involves the anterior hypothalamic area, the suprachiazmatic nucleus, and the rostral part of the lateral hypothalamic area.", "contents": "[Role of adreno- and cholinoreactive structures of the hypothalamus in regulating blood coagulation and maintaining it in a fluid state]. Electrical stimulation of anterior and lateral hypothalamus as well as acetylcholin injection into the same areas activated the anticoagulating blood system whereas adrenaline injections activated the coagulating system. The electrical stimulation with certain parameters seems to involve cholinoreactive structures of the hypothalamus, shifting the blood potential towards hypocoagulation. The sitmulation of the paraventricular and ventromedial hypothalamic nuclei with the same current activated the blood coagulating system whereas stimulation of the preoptic lateral or medial areas, medial supraoptic nucleus of the hypothalamus induced no changes in the homeostatic system. The data obtained suggest that the regulation of the blood anticoagulating system involves the anterior hypothalamic area, the suprachiazmatic nucleus, and the rostral part of the lateral hypothalamic area."}
{"id": "PMID:488458", "title": "[Effect of inspiratory resistance loading on temporal parameters of respiration in anesthetized cats].", "content": "In anesthetized cats, an increase in inspiratory resistance in conditions of growing hypercapnia entailed elongation of inspiration and of the whole respiratory cycle, as well as decrease of the volume threshold for cessation of inspiration. The latter occurred after bilateral vagotomy too, whereas no significant changes of the temporal parameters followed the increase in the resistance. The temporal rearrangements of the respiratory cycle seem to be due to the vagal effects, while the extravagal influences, probably the reflexes from the stretch receptors of intercostal muscles, are responsible for changes of the volume component in the relations characterizing the mechanism of cessation of inspiration.", "contents": "[Effect of inspiratory resistance loading on temporal parameters of respiration in anesthetized cats]. In anesthetized cats, an increase in inspiratory resistance in conditions of growing hypercapnia entailed elongation of inspiration and of the whole respiratory cycle, as well as decrease of the volume threshold for cessation of inspiration. The latter occurred after bilateral vagotomy too, whereas no significant changes of the temporal parameters followed the increase in the resistance. The temporal rearrangements of the respiratory cycle seem to be due to the vagal effects, while the extravagal influences, probably the reflexes from the stretch receptors of intercostal muscles, are responsible for changes of the volume component in the relations characterizing the mechanism of cessation of inspiration."}
{"id": "PMID:488461", "title": "[Glycerinized models of enterocytes and several of their properties].", "content": "The method for the preparation of glycerol-treated models of the enterocytes with preserved membrane has been described. Using such a simplified model of the enterocyte suspension allows membrane digestion and enzyme properties to be studied. Moreover, preserving the important properties of the native isolated cells the glycerol-treated enterocytes have a number of essential advantages as compared with other known preparations of the small intestinal mucosa. Some characteristics of the glycerol-treated models of the enterocytes have been described in the paper.", "contents": "[Glycerinized models of enterocytes and several of their properties]. The method for the preparation of glycerol-treated models of the enterocytes with preserved membrane has been described. Using such a simplified model of the enterocyte suspension allows membrane digestion and enzyme properties to be studied. Moreover, preserving the important properties of the native isolated cells the glycerol-treated enterocytes have a number of essential advantages as compared with other known preparations of the small intestinal mucosa. Some characteristics of the glycerol-treated models of the enterocytes have been described in the paper."}
{"id": "PMID:488466", "title": "In vivo activity of spleen cells from untreated syngeneic mice against Ehrlich ascites carcinoma.", "content": "Inbred CFW/L1 or BALB/c male mice were injected intraperitoneally with 1 X 10(5) EAC cells. They died in 98.6 or 100%, respectively, within 100 days of observation. Intraperitoneal injection of 6 X 10(7) syngeneic spleen cells from 6--9-week-old CFW/L1 or BALB/c donors 2.5--4 h after tumour inoculation significantly prolonged MST and 16.7 or 23.5% of mice, respectively, survived for 100 days. Smaller amounts of spleen cells, thymus cells, mixed spleen and thymus cells, or spleen cells from older donors did not show this effect. Homogenized spleen cells were also inactive. Antitumour activity of spleen cells was still evident when they were isolated from irradiated (2 000R) donors or were given 24 h after tumour inoculation. Spleen cells used for in vivo studies showed spontaneous cytotoxic activity in vitro against EAC cells in the 51Cr-release cell-mediated cytotoxicity test. The possible participation of NK cells in the observed in vivo antitumour activity of normal spleen cells is discussed.", "contents": "In vivo activity of spleen cells from untreated syngeneic mice against Ehrlich ascites carcinoma. Inbred CFW/L1 or BALB/c male mice were injected intraperitoneally with 1 X 10(5) EAC cells. They died in 98.6 or 100%, respectively, within 100 days of observation. Intraperitoneal injection of 6 X 10(7) syngeneic spleen cells from 6--9-week-old CFW/L1 or BALB/c donors 2.5--4 h after tumour inoculation significantly prolonged MST and 16.7 or 23.5% of mice, respectively, survived for 100 days. Smaller amounts of spleen cells, thymus cells, mixed spleen and thymus cells, or spleen cells from older donors did not show this effect. Homogenized spleen cells were also inactive. Antitumour activity of spleen cells was still evident when they were isolated from irradiated (2 000R) donors or were given 24 h after tumour inoculation. Spleen cells used for in vivo studies showed spontaneous cytotoxic activity in vitro against EAC cells in the 51Cr-release cell-mediated cytotoxicity test. The possible participation of NK cells in the observed in vivo antitumour activity of normal spleen cells is discussed."}
{"id": "PMID:488467", "title": "Norway rat haemoglobin phenotypes: which is the original?", "content": "On the basis of the incidence of haemoglobin alleles in different populations of rats, the hypothesis is discussed that the HbbB is phylogenetically \"older\".", "contents": "Norway rat haemoglobin phenotypes: which is the original? On the basis of the incidence of haemoglobin alleles in different populations of rats, the hypothesis is discussed that the HbbB is phylogenetically \"older\"."}
{"id": "PMID:488468", "title": "The frequency of chromosomal aberrations in long-term irradiated mice and rats.", "content": "The frequency of chromosomal aberrations was studied in myelokaryocytes of mice and rats continuously irradiated for 90 days at a dose rate of 50R/day. The curves expressing the frequency of chromosomal aberrations in continuously irradiated mice and rats are biphasic. The first phase is similar for the two species and is characterized by an increase in the frequency of cells with chromosomal aberrations. In the second phase, the frequency of cells with chromosomal aberrations had stabilized at a level of 25--28% in rats, and in mice the values of aberrant cells were gradually increasing until the end of irradiation when they reached 40.1%. In contrast to it, the distribution of aberrations of the chromatid and chromosomal types did not change throughout the experiment in both animal species.", "contents": "The frequency of chromosomal aberrations in long-term irradiated mice and rats. The frequency of chromosomal aberrations was studied in myelokaryocytes of mice and rats continuously irradiated for 90 days at a dose rate of 50R/day. The curves expressing the frequency of chromosomal aberrations in continuously irradiated mice and rats are biphasic. The first phase is similar for the two species and is characterized by an increase in the frequency of cells with chromosomal aberrations. In the second phase, the frequency of cells with chromosomal aberrations had stabilized at a level of 25--28% in rats, and in mice the values of aberrant cells were gradually increasing until the end of irradiation when they reached 40.1%. In contrast to it, the distribution of aberrations of the chromatid and chromosomal types did not change throughout the experiment in both animal species."}
{"id": "PMID:488486", "title": "[Dermatoses provoked by opportunistic fungi].", "content": "In my expos\u00e9 I confined myself to the moulds. Generally speaking, there are two groups of fungi. One group that often provokes diseases in otherwise normal subjects (fungi of parasitic-pathogenic character) and a second group that causes diseases in cases of lowered immunobiological resistance (fungi of saprophytic-opportunistic character). Though the biological conditions of the host are important, they are by no means the only prerequisites for the development of a mycosis. Just as important are the enzymatic qualities of the fungus. Ubiquitous moulds can become opportunists and provoke dermatoses under two conditions: (1) The fungus must possess keratolytic capabilities (2) its penetration and growth must be preceded by a lesion due to trauma, or bacterial or viral infection.", "contents": "[Dermatoses provoked by opportunistic fungi]. In my expos\u00e9 I confined myself to the moulds. Generally speaking, there are two groups of fungi. One group that often provokes diseases in otherwise normal subjects (fungi of parasitic-pathogenic character) and a second group that causes diseases in cases of lowered immunobiological resistance (fungi of saprophytic-opportunistic character). Though the biological conditions of the host are important, they are by no means the only prerequisites for the development of a mycosis. Just as important are the enzymatic qualities of the fungus. Ubiquitous moulds can become opportunists and provoke dermatoses under two conditions: (1) The fungus must possess keratolytic capabilities (2) its penetration and growth must be preceded by a lesion due to trauma, or bacterial or viral infection."}
{"id": "PMID:488487", "title": "[Research of keratinophiles in the soil and their effects on human cutaneous pathology in the Lyons area].", "content": "Between 1967 and 1977, the systematic research of keratinophiles of the ground in a radius of 30--50 km around Lyon, has shown the ubiquitarian predominance of Microsporum nanum, which is probably the most ancient occupant of the ground. The most intensive areas of human and animal occupation in the Rhodanian tract show the predominance of Microsporum gypseum, which is scarcely found in man. Sporadic localizations, tightly limites, bound to cirulcation axes and international gathering, let us detect Microsporum cookei, Trichophyton verrucosum, Trichophyton mentagrophytes, Chrysosporium keratinophilum, and Keratinomyces ajelloi. However, in the last years, a progressive extension of recently imported kinds and a decrease in native kinds appears to have occurred.", "contents": "[Research of keratinophiles in the soil and their effects on human cutaneous pathology in the Lyons area]. Between 1967 and 1977, the systematic research of keratinophiles of the ground in a radius of 30--50 km around Lyon, has shown the ubiquitarian predominance of Microsporum nanum, which is probably the most ancient occupant of the ground. The most intensive areas of human and animal occupation in the Rhodanian tract show the predominance of Microsporum gypseum, which is scarcely found in man. Sporadic localizations, tightly limites, bound to cirulcation axes and international gathering, let us detect Microsporum cookei, Trichophyton verrucosum, Trichophyton mentagrophytes, Chrysosporium keratinophilum, and Keratinomyces ajelloi. However, in the last years, a progressive extension of recently imported kinds and a decrease in native kinds appears to have occurred."}
{"id": "PMID:488488", "title": "[Otomycoses].", "content": "Otomycosis is a relatively frequent disorder (21.7% of the cases of external otitis) of fungi origin. Otomycoses can be discriminated from bacterial otitis by the following symptoms: itching, absence of otalgy, discharge and negative response to different local antibiotic therapies. Our study involves 148 cases of otomycosis and their therapy. Recovery was obtained in approximately 10 days.", "contents": "[Otomycoses]. Otomycosis is a relatively frequent disorder (21.7% of the cases of external otitis) of fungi origin. Otomycoses can be discriminated from bacterial otitis by the following symptoms: itching, absence of otalgy, discharge and negative response to different local antibiotic therapies. Our study involves 148 cases of otomycosis and their therapy. Recovery was obtained in approximately 10 days."}
{"id": "PMID:488489", "title": "[Phycomycoses].", "content": "Phycomycoses are opportunistic fungal diseases as far as mucormycosis is concerned, but for basidiobolomycosis and entomophthoromycosis, the adjective 'opportunistic' should be used with caution. Mucormycosis assumes cranial, pulmonary, gastrointestinal, disseminated, cutaneous and focal forms: the infection nearly always develops in a person whose resistance is lowered by a metabolic disorder, a blood dyscrasia, corticosteroid or immunodepressive therapy. It is a frequent complication in leukemia or diabetic acidosis. Basidiobolomycosis and entomophtoromycosis are caused by fungi which belong to the order, Entomophthorales. They are characterized in tissue by broad hyphae which are surrounded by eosinophilic material. These mycoses are treated by potassium iodide.", "contents": "[Phycomycoses]. Phycomycoses are opportunistic fungal diseases as far as mucormycosis is concerned, but for basidiobolomycosis and entomophthoromycosis, the adjective 'opportunistic' should be used with caution. Mucormycosis assumes cranial, pulmonary, gastrointestinal, disseminated, cutaneous and focal forms: the infection nearly always develops in a person whose resistance is lowered by a metabolic disorder, a blood dyscrasia, corticosteroid or immunodepressive therapy. It is a frequent complication in leukemia or diabetic acidosis. Basidiobolomycosis and entomophtoromycosis are caused by fungi which belong to the order, Entomophthorales. They are characterized in tissue by broad hyphae which are surrounded by eosinophilic material. These mycoses are treated by potassium iodide."}
{"id": "PMID:488490", "title": "[Therapy of opportunistic mycoses].", "content": "The author presents the drugs that are available for the treatment of opportunistic mycoses: amphotericin B, nystatin, 5-fluorocytosine, miconazole and the newest imidazole derivative econazole. He presents his experience with econazole in 4 cases with deep mycoses. He speaks of the mode of application and the therapeutical limits of these products as well as of the favorable factors and the prophylactic measures to be taken.", "contents": "[Therapy of opportunistic mycoses]. The author presents the drugs that are available for the treatment of opportunistic mycoses: amphotericin B, nystatin, 5-fluorocytosine, miconazole and the newest imidazole derivative econazole. He presents his experience with econazole in 4 cases with deep mycoses. He speaks of the mode of application and the therapeutical limits of these products as well as of the favorable factors and the prophylactic measures to be taken."}
{"id": "PMID:488491", "title": "[Parasitic adaptation phenomena of pathogenic fungi in human and veterinary medicine].", "content": "The pathogen species involved in medical and veterinary mycology can be divided into three categories according to their level of increasing adaptation to parasitism. Only the third category includes a few species (some dermatophytes) which can be regarded as genuine parasites. The opportunistic fungi belong to the first or the first two the third categories according to the given definition. The various possible factors of preadaptation or adaptation to parasitic life (physical factors, biochemical factors, avoidance of the immunological pressure and ecological factors) are discussed. Except for the species belonging to the first category the probably are efficient only if the host does not react normally.", "contents": "[Parasitic adaptation phenomena of pathogenic fungi in human and veterinary medicine]. The pathogen species involved in medical and veterinary mycology can be divided into three categories according to their level of increasing adaptation to parasitism. Only the third category includes a few species (some dermatophytes) which can be regarded as genuine parasites. The opportunistic fungi belong to the first or the first two the third categories according to the given definition. The various possible factors of preadaptation or adaptation to parasitic life (physical factors, biochemical factors, avoidance of the immunological pressure and ecological factors) are discussed. Except for the species belonging to the first category the probably are efficient only if the host does not react normally."}
{"id": "PMID:488492", "title": "[Opportunism in dermatophytes].", "content": "Dematophytes invade hosts when their state is suitable for the production of their pathogenicity. When the host's resistance is reduced, the prevalence of dermatophytoses can be extremely high or the symptomatology can be very unusual. It is suspected that the 'maladie dermatophytique' and pherhaps dermatophytic mycetoma develop in such situations. The fact is that the behaviour of dermatophytes does not differ from that of the so-called opportunistic fungi and in some cases it is possible to speak of dermatophytic asthenomycosis.", "contents": "[Opportunism in dermatophytes]. Dematophytes invade hosts when their state is suitable for the production of their pathogenicity. When the host's resistance is reduced, the prevalence of dermatophytoses can be extremely high or the symptomatology can be very unusual. It is suspected that the 'maladie dermatophytique' and pherhaps dermatophytic mycetoma develop in such situations. The fact is that the behaviour of dermatophytes does not differ from that of the so-called opportunistic fungi and in some cases it is possible to speak of dermatophytic asthenomycosis."}
{"id": "PMID:488493", "title": "[Soil as a reservoir of opportunistic mycostic agents].", "content": "We studied the soil as a reservoir of vegetable detritus, keratinous materials of birds, terrestrial animals and micro-organisms. In the last group we can mention the bacteria and fungi. We classified the opportunistic fungi in three groups: (1) parasites of plants or growing on the vegetable detritus; (2) geophylic dermatophytes; (3) pathogenic fungi that in some cases behave like opportunistic ones. In each group we studied the opportunistic fungi of the soil, their relations with it chemical and physical composition, the mycoflora and the role of animal habitats.", "contents": "[Soil as a reservoir of opportunistic mycostic agents]. We studied the soil as a reservoir of vegetable detritus, keratinous materials of birds, terrestrial animals and micro-organisms. In the last group we can mention the bacteria and fungi. We classified the opportunistic fungi in three groups: (1) parasites of plants or growing on the vegetable detritus; (2) geophylic dermatophytes; (3) pathogenic fungi that in some cases behave like opportunistic ones. In each group we studied the opportunistic fungi of the soil, their relations with it chemical and physical composition, the mycoflora and the role of animal habitats."}
{"id": "PMID:488494", "title": "[Alimentary nickel and contact eczema (author's transl)].", "content": "By atomic absorption spectrometry, nickel was analysed in many foodstuffs. The results of these determinations are given in detail. Nickel present in vegetables comes primarily from the soil but secondarily from stainless-steel cooking utensils.", "contents": "[Alimentary nickel and contact eczema (author's transl)]. By atomic absorption spectrometry, nickel was analysed in many foodstuffs. The results of these determinations are given in detail. Nickel present in vegetables comes primarily from the soil but secondarily from stainless-steel cooking utensils."}
{"id": "PMID:488495", "title": "[Ultrastructure of epidermolyses with junctional blister formation (author's transl)].", "content": "Junctional blister formation is the common feature of four clinically different types of inherited epidermolyses with atrophies but lack of scarring which, as a group, have been termed epidermolysis bullosa atrophicans. The Herlitz type is the most common one among them. Based on a material of 24 cases aged 2 days to 42 years at the time of biopsy, ultrastructural data on the pathomorphogenesis are reported. Blisters form in the dermo-epidermal junction area between the basal lamina and the basal cell plasma membrane. A well-developed basal lamina-anchoring fibrils complex covers the blister floor. Hypoplasia of hemidesmosomes precedes blister formation and is a constant finding in intact junction area without any other signs of disturbances. It is, therefore, regarded as a causal factor in the pathogenesis. Nail loss as a result of junctional separation within the nail bed, secondary changes in older blisters and formation of slowly healing granulation tissues are also reported.--Early diagnosis is made available by electron microscopy as shown in out early childhood cases. Genes coding for junctional epidermolyses seem to be far more frequent than those coding for the scarring or dystrophic types.", "contents": "[Ultrastructure of epidermolyses with junctional blister formation (author's transl)]. Junctional blister formation is the common feature of four clinically different types of inherited epidermolyses with atrophies but lack of scarring which, as a group, have been termed epidermolysis bullosa atrophicans. The Herlitz type is the most common one among them. Based on a material of 24 cases aged 2 days to 42 years at the time of biopsy, ultrastructural data on the pathomorphogenesis are reported. Blisters form in the dermo-epidermal junction area between the basal lamina and the basal cell plasma membrane. A well-developed basal lamina-anchoring fibrils complex covers the blister floor. Hypoplasia of hemidesmosomes precedes blister formation and is a constant finding in intact junction area without any other signs of disturbances. It is, therefore, regarded as a causal factor in the pathogenesis. Nail loss as a result of junctional separation within the nail bed, secondary changes in older blisters and formation of slowly healing granulation tissues are also reported.--Early diagnosis is made available by electron microscopy as shown in out early childhood cases. Genes coding for junctional epidermolyses seem to be far more frequent than those coding for the scarring or dystrophic types."}
{"id": "PMID:488496", "title": "[Delayed hypersensitivity against staphylococcal antigens in guinea pigs (author's transl)].", "content": "Delayed type hypersensitivity against staphylococcal antigens could be induced in guinea pigs by injecting the animals with a staphylococcal homogenate in Freund's adjuvant in all four foot pads and the nuchal skin. Maximal skin reactivity, tested intracutaneously, was observed 21 days after sensitization. Highest stimulation of lymph node cells and peripheral blood lymphocytes was also obtained 21 days after sensitization. When comparing cell walls, cell materials (nonsoluble material obtained after the separation of the cell wall fraction) and soluble fraction (obtained after sedimentation of all nonsoluble material, the strongest skin test reactions occurred after testing intracutaneously with the cell wall fraction.", "contents": "[Delayed hypersensitivity against staphylococcal antigens in guinea pigs (author's transl)]. Delayed type hypersensitivity against staphylococcal antigens could be induced in guinea pigs by injecting the animals with a staphylococcal homogenate in Freund's adjuvant in all four foot pads and the nuchal skin. Maximal skin reactivity, tested intracutaneously, was observed 21 days after sensitization. Highest stimulation of lymph node cells and peripheral blood lymphocytes was also obtained 21 days after sensitization. When comparing cell walls, cell materials (nonsoluble material obtained after the separation of the cell wall fraction) and soluble fraction (obtained after sedimentation of all nonsoluble material, the strongest skin test reactions occurred after testing intracutaneously with the cell wall fraction."}
{"id": "PMID:488497", "title": "[Clinic and treatment of porphyria cutanea tarda (author's transl)].", "content": "Symptomatology and pathogenesis of porphyria cutanea tarda (PCT) are shortly reviewed. The different possibilities in the treatment of PCT are discussed with special reference to the phlebotomy according to Ippen and to the chloroquine administration according to the regimen described by Kordac and Semradova in 1974. 75 PCT patients were treated with chloroquine (Resochin, Nivaquine). After 9.3 +/- 3.4 months (means +/- SE) all patients excreted normal amounts of porphyrins, and the clinical symptoms disappeared. 14 of these patients relapsed after a mean period of 21 months after the withdrawal of chloroquine.", "contents": "[Clinic and treatment of porphyria cutanea tarda (author's transl)]. Symptomatology and pathogenesis of porphyria cutanea tarda (PCT) are shortly reviewed. The different possibilities in the treatment of PCT are discussed with special reference to the phlebotomy according to Ippen and to the chloroquine administration according to the regimen described by Kordac and Semradova in 1974. 75 PCT patients were treated with chloroquine (Resochin, Nivaquine). After 9.3 +/- 3.4 months (means +/- SE) all patients excreted normal amounts of porphyrins, and the clinical symptoms disappeared. 14 of these patients relapsed after a mean period of 21 months after the withdrawal of chloroquine."}
{"id": "PMID:488499", "title": "[Clinical aspects of epidermolyses with junctional blister formation (author's transl)].", "content": "The clinical symptomatology of epidermolysis bullosa atrophicans generalisata gravis (Herlitz' disease), epidermolysis bullosa atrophicans generalisata mitis (typus Disentis) and epidermolysis bullosa atrophicans localisata is described. These types belong to the non-scarring epidermolyses and show ultrastructurally junctional blistering.", "contents": "[Clinical aspects of epidermolyses with junctional blister formation (author's transl)]. The clinical symptomatology of epidermolysis bullosa atrophicans generalisata gravis (Herlitz' disease), epidermolysis bullosa atrophicans generalisata mitis (typus Disentis) and epidermolysis bullosa atrophicans localisata is described. These types belong to the non-scarring epidermolyses and show ultrastructurally junctional blistering."}
{"id": "PMID:488520", "title": "Biological correlates of piracetam clinical effects in psychotic patients.", "content": "The purpose of this controlled clinical trial was to demonstrate possible correlations between changes in bioenergetic metabolism and psychotropic drug administration in the treatment of functional psychosis. The study included twenty-six patients, eleven with schizophrenia, three with chronic atypical depression and twelve with drug-resistant endogenous depressions. All patients were kept on continuous psychotropic medication for at least 3 weeks before starting the trial, and piracetam was given additionally in a fixed dosage of 2400 mg daily; the same number of identical capsules was given during the pre- and post-treatment placebo periods. Psycho-pathological evaluation of the patients was by the BPRS; clinical and biochemical data were evaluated statistically by the analysis of regression. The results show that in schizophrenic patients an improvement was observed in those cases who had improved biochemically, i.e. where the ATP values had increased. In drug-resistant depressions there was a rapid and significant clinical improvement after piracetam co-administration, and this went in step with a significant rise in ATP levels.", "contents": "Biological correlates of piracetam clinical effects in psychotic patients. The purpose of this controlled clinical trial was to demonstrate possible correlations between changes in bioenergetic metabolism and psychotropic drug administration in the treatment of functional psychosis. The study included twenty-six patients, eleven with schizophrenia, three with chronic atypical depression and twelve with drug-resistant endogenous depressions. All patients were kept on continuous psychotropic medication for at least 3 weeks before starting the trial, and piracetam was given additionally in a fixed dosage of 2400 mg daily; the same number of identical capsules was given during the pre- and post-treatment placebo periods. Psycho-pathological evaluation of the patients was by the BPRS; clinical and biochemical data were evaluated statistically by the analysis of regression. The results show that in schizophrenic patients an improvement was observed in those cases who had improved biochemically, i.e. where the ATP values had increased. In drug-resistant depressions there was a rapid and significant clinical improvement after piracetam co-administration, and this went in step with a significant rise in ATP levels."}
{"id": "PMID:488522", "title": "Multicentre general practitioner assessment of 'Tenormin' and methyldopa.", "content": "A large scale study in general practice was set up to investigate the effects of transferring hypertensive patients from treatment with usually less than 1 g daily of methyldopa to atenolol ('Tenormin') 100 mg daily. The results demonstrate an improvement in blood pressure control with atenolol treatment and a reduction in the incidence of side-effects. The simple dosage regime, combined with proven effectiveness and a relative lack of side-effects makes atenolol a useful treatment for the hypertensive patient.", "contents": "Multicentre general practitioner assessment of 'Tenormin' and methyldopa. A large scale study in general practice was set up to investigate the effects of transferring hypertensive patients from treatment with usually less than 1 g daily of methyldopa to atenolol ('Tenormin') 100 mg daily. The results demonstrate an improvement in blood pressure control with atenolol treatment and a reduction in the incidence of side-effects. The simple dosage regime, combined with proven effectiveness and a relative lack of side-effects makes atenolol a useful treatment for the hypertensive patient."}
{"id": "PMID:488523", "title": "Insulin requirements in diabetic patients treated with sulphinpyrazone.", "content": "Forty-one adult diabetic patients of either sex who had been controlled by insulin alone for at least one year were randomly allocated in a double-blind, between-patient study, to either sulphinpyrazone (600--800 mg daily) or an identnd at the end of it, no clinically or statistically significant change of the insulin needs of the patients was observed. It is concluded that no clinical interaction occurs between sulphinpyrazone (800 mg/day or 600 mg/day) and insulin when they are administered simultaneously for long periods. In the doses used the tolerability of sulphinpyrazone was very good.", "contents": "Insulin requirements in diabetic patients treated with sulphinpyrazone. Forty-one adult diabetic patients of either sex who had been controlled by insulin alone for at least one year were randomly allocated in a double-blind, between-patient study, to either sulphinpyrazone (600--800 mg daily) or an identnd at the end of it, no clinically or statistically significant change of the insulin needs of the patients was observed. It is concluded that no clinical interaction occurs between sulphinpyrazone (800 mg/day or 600 mg/day) and insulin when they are administered simultaneously for long periods. In the doses used the tolerability of sulphinpyrazone was very good."}
{"id": "PMID:488525", "title": "Effect of glucocorticoid levels in vivo on growth hormone biosynthesis.", "content": "The rate of growth hormone (GH) biosynthesis in pituitary cells prepared from rats with different histories of glucocorticoid exposure was analyzed by a dual-labeling acrylamide-gel technique. Glucocorticoid deficiency, produced by adrenalectomy, reduces GH synthesis by approximately 50%. GH synthesis adrenalectomized rats treated with either natural or synthetic glucocorticoids, is restored to normal or above normal levels. Acute exposure (1 h) of cells to corticosterone in vitro does not produce a significant increase in GH synthesis. Adrenalectomized rats treated with steroid for one day show a small but detectable increase in GH synthesis, while treatment for 3--6 days results in progressively larger stimulatory effects. All of these results are similar to previous findings regarding glucocorticoid stimulation of GH synthesis in vitro.", "contents": "Effect of glucocorticoid levels in vivo on growth hormone biosynthesis. The rate of growth hormone (GH) biosynthesis in pituitary cells prepared from rats with different histories of glucocorticoid exposure was analyzed by a dual-labeling acrylamide-gel technique. Glucocorticoid deficiency, produced by adrenalectomy, reduces GH synthesis by approximately 50%. GH synthesis adrenalectomized rats treated with either natural or synthetic glucocorticoids, is restored to normal or above normal levels. Acute exposure (1 h) of cells to corticosterone in vitro does not produce a significant increase in GH synthesis. Adrenalectomized rats treated with steroid for one day show a small but detectable increase in GH synthesis, while treatment for 3--6 days results in progressively larger stimulatory effects. All of these results are similar to previous findings regarding glucocorticoid stimulation of GH synthesis in vitro."}
{"id": "PMID:488526", "title": "Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase.", "content": "Ecdysone 20-monooxygenase, the enzyme system that hydroxylates ecdysone at C-20 of the side-chain to form ecdysterone, has been characterized in the fat body of early last instar larvae of the tobacco hornworm, Manduca sexta, using a radioenzymological assay. Ecdysterone was demonstrated to be the product of the enzyme system by high-pressure liquid chromatography, gas-liquid chromatography and mass spectrometry. Differential centrifugation, sucrose-gradient centrifugation, electron microscopy and organelle-marker enzyme analysis revealed that ecdysone 20-monooxygenase activity is associated with the mitochondria. The enzymatic properties of ecdysone 20-monooxygenase are that it is most active in a 0.05 M phosphate buffer, is inhibited by Mg2+ and exhibits pH and temperature optima at 7.5 and 30 degrees C, respectively. The enzyme complex has an apparent Km for ecdysone of 1.60 x 10(-7) M and is competitively inhibited by its product, ecdysterone, with an apparent Ki of 2.72 x 10(-5) M. The cytochrome P-450 nature of this insect steroid hydroxylase was initially suggested by its obligate requirement for NADPH and its inhibition by carbon monoxide, p-chloromercuribenzoate, metyrapone and p-aminoglutethimide but not by cyanide. Difference spectroscopy revealed the presence of cytochrome P-450 in the fat-body mitochondrial fraction. A photochemical action spectrum of ecdysone 20-monooxygenase activity confirmed the involvement of cytochrome P-450 in this monooxygenase system.", "contents": "Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase. Ecdysone 20-monooxygenase, the enzyme system that hydroxylates ecdysone at C-20 of the side-chain to form ecdysterone, has been characterized in the fat body of early last instar larvae of the tobacco hornworm, Manduca sexta, using a radioenzymological assay. Ecdysterone was demonstrated to be the product of the enzyme system by high-pressure liquid chromatography, gas-liquid chromatography and mass spectrometry. Differential centrifugation, sucrose-gradient centrifugation, electron microscopy and organelle-marker enzyme analysis revealed that ecdysone 20-monooxygenase activity is associated with the mitochondria. The enzymatic properties of ecdysone 20-monooxygenase are that it is most active in a 0.05 M phosphate buffer, is inhibited by Mg2+ and exhibits pH and temperature optima at 7.5 and 30 degrees C, respectively. The enzyme complex has an apparent Km for ecdysone of 1.60 x 10(-7) M and is competitively inhibited by its product, ecdysterone, with an apparent Ki of 2.72 x 10(-5) M. The cytochrome P-450 nature of this insect steroid hydroxylase was initially suggested by its obligate requirement for NADPH and its inhibition by carbon monoxide, p-chloromercuribenzoate, metyrapone and p-aminoglutethimide but not by cyanide. Difference spectroscopy revealed the presence of cytochrome P-450 in the fat-body mitochondrial fraction. A photochemical action spectrum of ecdysone 20-monooxygenase activity confirmed the involvement of cytochrome P-450 in this monooxygenase system."}
{"id": "PMID:488527", "title": "Ontogeny of maternal behavior in the laboratory rat: early origins in 18- to 27-day-old young.", "content": "The responses of unweaned juveniles, 18 to 27 days of age, in brief tests with pups younger than 10 days were observed during initial exposure and at 4-6-day intervals. They showed no aversive responses and actively sought contact with the pups until 24 days when contact-seeking declined sharply. Young exposed to pups before 24 days continued to seek contact with them after 24 days showing an effect of the prior experience. Juveniles given a choice between young pups and a warm bowl chose the pups; given a choice between young pups and age mates, they chose the young pups until day 23, but chose the age mates after then. When housed continuously with young pups at 22 days of age, 5 of 9 retrieved and grouped pups with a latency of 1 day. Our results indicate that timidity or fear of novelty develops in rats in this situation about Day 24 and thereafter is a factor inhibiting the expression of positive social behavior toward pups.", "contents": "Ontogeny of maternal behavior in the laboratory rat: early origins in 18- to 27-day-old young. The responses of unweaned juveniles, 18 to 27 days of age, in brief tests with pups younger than 10 days were observed during initial exposure and at 4-6-day intervals. They showed no aversive responses and actively sought contact with the pups until 24 days when contact-seeking declined sharply. Young exposed to pups before 24 days continued to seek contact with them after 24 days showing an effect of the prior experience. Juveniles given a choice between young pups and a warm bowl chose the pups; given a choice between young pups and age mates, they chose the young pups until day 23, but chose the age mates after then. When housed continuously with young pups at 22 days of age, 5 of 9 retrieved and grouped pups with a latency of 1 day. Our results indicate that timidity or fear of novelty develops in rats in this situation about Day 24 and thereafter is a factor inhibiting the expression of positive social behavior toward pups."}
{"id": "PMID:488528", "title": "Ontogeny of maternal behavior in the laboratory rat: factors underlying changes in responsiveness from 30 to 90 days.", "content": "Male and female rats that were nonhandled or that were handled from weaning, and that had intact or impaired olfaction (intranasal zinc sulfate), were sensitized through continuous pup exposure commencing at 30, 45, 60, or 90 days of age. Nonhandled males and females were alike in latencies to become maternal at Day 30 but thereafter latencies of females became shorter and latencies of males lengthened; by 90 days males had markedly longer latencies than females and only 1/3 became maternal. Handling facilitated sensitization at 30 days among males and females but only among males at 45 and 60 days. Intranasal zinc sulfate reduced latencies of both males and females at all ages tested, but appeared most effective after 45 days of age. Gender differences in latencies persisted in adult animals even after combined treatments. Results indicate that both timidity and olfaction inhibit the onset of maternal responses to pups in virgin males and females, but they differ in relative importance by age and gender.", "contents": "Ontogeny of maternal behavior in the laboratory rat: factors underlying changes in responsiveness from 30 to 90 days. Male and female rats that were nonhandled or that were handled from weaning, and that had intact or impaired olfaction (intranasal zinc sulfate), were sensitized through continuous pup exposure commencing at 30, 45, 60, or 90 days of age. Nonhandled males and females were alike in latencies to become maternal at Day 30 but thereafter latencies of females became shorter and latencies of males lengthened; by 90 days males had markedly longer latencies than females and only 1/3 became maternal. Handling facilitated sensitization at 30 days among males and females but only among males at 45 and 60 days. Intranasal zinc sulfate reduced latencies of both males and females at all ages tested, but appeared most effective after 45 days of age. Gender differences in latencies persisted in adult animals even after combined treatments. Results indicate that both timidity and olfaction inhibit the onset of maternal responses to pups in virgin males and females, but they differ in relative importance by age and gender."}
{"id": "PMID:488529", "title": "Ontogeny of ultradian and nocturnal activity rhythms in the isolated albino rat.", "content": "The activity of isolated rats from 6 to 25 days of age was analyzed at 10-min intervals. Ultradian peak activity rhythms, with a rough 1-2-hr periodicity, developed between 9 and 12 days of age, and reached an extreme at 15 days. Ultradian periods of attenuated activity were however detectable even in 6-day-olds. Isolated rats at 15 days of age displayed greatly accentuated profiles characterized not only by heightened activity levels, but by an extreme cyclicity. These subjects spent most of their time oscillating between periods of marked activity and marked inactivity. Both the average and total duration of these extreme periods were greater in 15-day-olds than any of the other ages. Free-running nocturnal activity rhythms were also observed to develop in isolated rats tested in continuous darkness by the 15th day.", "contents": "Ontogeny of ultradian and nocturnal activity rhythms in the isolated albino rat. The activity of isolated rats from 6 to 25 days of age was analyzed at 10-min intervals. Ultradian peak activity rhythms, with a rough 1-2-hr periodicity, developed between 9 and 12 days of age, and reached an extreme at 15 days. Ultradian periods of attenuated activity were however detectable even in 6-day-olds. Isolated rats at 15 days of age displayed greatly accentuated profiles characterized not only by heightened activity levels, but by an extreme cyclicity. These subjects spent most of their time oscillating between periods of marked activity and marked inactivity. Both the average and total duration of these extreme periods were greater in 15-day-olds than any of the other ages. Free-running nocturnal activity rhythms were also observed to develop in isolated rats tested in continuous darkness by the 15th day."}
{"id": "PMID:488530", "title": "Approach and departure patterns generated in the domestic chick by intermittent white light.", "content": "Newly hatched domestic chicks were given extended free access to 7 rates of intermittent white light between .5 and 24 flashes per sec. Inspection of the data showed approaches and departures directed toward the light that increased in number and formed an orderly pattern as the rates neared 4 per sec and as the chicks exposed to this rate became older. Also, no differences were found in the amount of time chicks remained next to the various rates during each approach but only in the amount of time they remained away during each departure. The bearing of these results on the proposal that flicker phototaxis might stem from the neural excitation produced through photic driving was discussed along with relating further evidence to recent work in imprinting.", "contents": "Approach and departure patterns generated in the domestic chick by intermittent white light. Newly hatched domestic chicks were given extended free access to 7 rates of intermittent white light between .5 and 24 flashes per sec. Inspection of the data showed approaches and departures directed toward the light that increased in number and formed an orderly pattern as the rates neared 4 per sec and as the chicks exposed to this rate became older. Also, no differences were found in the amount of time chicks remained next to the various rates during each approach but only in the amount of time they remained away during each departure. The bearing of these results on the proposal that flicker phototaxis might stem from the neural excitation produced through photic driving was discussed along with relating further evidence to recent work in imprinting."}
{"id": "PMID:488531", "title": "Postnatal undernutrition in rats: attempts to develop alternative methods to food deprive pups without maternal behavioral alteration.", "content": "Two methods were investigated as attempts to undernourish rat pups without the disturbances in maternal behavior that accompany the procedures used to date for this purpose. In the 1st method, a litter of 12 pups was raised by both a lactating mother and a \"sensitized\" female. The sensitized female was provided under the assumption that she could correct for the deficit in maternal care when 1 mother raises a large litter. The results showed that the pups raised by the 2 females were constantly removed by the females from each other's nests; the females engaged in constant fighting and showed altered maternal behavior. As a consequence the pups lost more weight than control underfed young. The 2nd method consisted of removing 6-8 nipples from virgin females which were mated 10 days later. After delivery these females raised litters of 6 pups. Their maternal behavior was equal to that of unoperated controls, and at weaning the pups had 20-50% less body weight. This method could be useful to study undernutrition effects on behavior, without confounding experimental variables.", "contents": "Postnatal undernutrition in rats: attempts to develop alternative methods to food deprive pups without maternal behavioral alteration. Two methods were investigated as attempts to undernourish rat pups without the disturbances in maternal behavior that accompany the procedures used to date for this purpose. In the 1st method, a litter of 12 pups was raised by both a lactating mother and a \"sensitized\" female. The sensitized female was provided under the assumption that she could correct for the deficit in maternal care when 1 mother raises a large litter. The results showed that the pups raised by the 2 females were constantly removed by the females from each other's nests; the females engaged in constant fighting and showed altered maternal behavior. As a consequence the pups lost more weight than control underfed young. The 2nd method consisted of removing 6-8 nipples from virgin females which were mated 10 days later. After delivery these females raised litters of 6 pups. Their maternal behavior was equal to that of unoperated controls, and at weaning the pups had 20-50% less body weight. This method could be useful to study undernutrition effects on behavior, without confounding experimental variables."}
{"id": "PMID:488532", "title": "The effect of visual deprivation on pattern recognition in the rat.", "content": "In 2 experiments the performances of light-reared (LR) and dark-reared (DR) 90-day-old rats were compared on a variety of transfer tests following acquisition of 3 pattern discriminations. Significant differences due to rearing condition were found in performance on a discrimination involving horizontal separations between pairs of vertical contours. No such differences due to rearing were observed during either acquisition or transfer testing of a discrimination involving pairs of mirror image oblique contours. Significant differences due to rearing were observed in acquisition of a discrimination between a checkerboard pattern and a similar pattern containing an irregularity and in performance on a subsequent series of transfer tests. The results suggest that the spatial integration necessary to discriminate basic as well as complex relational properties between linear elements involves mechanisms which require experience for their development and maintenance.", "contents": "The effect of visual deprivation on pattern recognition in the rat. In 2 experiments the performances of light-reared (LR) and dark-reared (DR) 90-day-old rats were compared on a variety of transfer tests following acquisition of 3 pattern discriminations. Significant differences due to rearing condition were found in performance on a discrimination involving horizontal separations between pairs of vertical contours. No such differences due to rearing were observed during either acquisition or transfer testing of a discrimination involving pairs of mirror image oblique contours. Significant differences due to rearing were observed in acquisition of a discrimination between a checkerboard pattern and a similar pattern containing an irregularity and in performance on a subsequent series of transfer tests. The results suggest that the spatial integration necessary to discriminate basic as well as complex relational properties between linear elements involves mechanisms which require experience for their development and maintenance."}
{"id": "PMID:488533", "title": "Deficits in fixed-interval performance following prenatal and postnatal lead exposure.", "content": "Female rats were exposed daily to 750 mg/kg of lead acetate via a restricted watering schedule for 70-80 days prior to mating and then throughout pregnancy and nursing. At weaning, litters from half of the lead dams were placed directly on treatment for the duration of the experiment. These manipulations yielded 3 groups: Group Pb/Pb, offspring exposed during pre- and postweaning  periods; Group Pb/C, offspring exposed only during preweaning periods: and Group C/C, control offspring. Beginning at 42-49 days of age, postnatal, offspring were shaped to bar press under a fixed-interval, 1-min schedule (FI1) and then given 20 sessions, each 45 min in length. Analyses revealed that Group Pb/Pb received fewere reinforcements across sessions than the other 2 groups, which did not differ. However, when the Pb/Pb offspring did receive reinforcement, they exhibited the scalloped pattern of responding characteristic of fixed-interval schedules.", "contents": "Deficits in fixed-interval performance following prenatal and postnatal lead exposure. Female rats were exposed daily to 750 mg/kg of lead acetate via a restricted watering schedule for 70-80 days prior to mating and then throughout pregnancy and nursing. At weaning, litters from half of the lead dams were placed directly on treatment for the duration of the experiment. These manipulations yielded 3 groups: Group Pb/Pb, offspring exposed during pre- and postweaning  periods; Group Pb/C, offspring exposed only during preweaning periods: and Group C/C, control offspring. Beginning at 42-49 days of age, postnatal, offspring were shaped to bar press under a fixed-interval, 1-min schedule (FI1) and then given 20 sessions, each 45 min in length. Analyses revealed that Group Pb/Pb received fewere reinforcements across sessions than the other 2 groups, which did not differ. However, when the Pb/Pb offspring did receive reinforcement, they exhibited the scalloped pattern of responding characteristic of fixed-interval schedules."}
{"id": "PMID:488534", "title": "Long-term effects of preweaning taurine injections in rats: interactions with strain and gender.", "content": "A comparison was made of MR, MNR, and RCA strains that had been injected with either 62.5 mg/kg of taurine or comparable volumes of physiological saline between postnatal Days 8 and 20 and later tested at 100 days of age in an open field and 1-way shock avoidance situations. Taurine-injected rats displayed significant elevation in defecation score in the open field. In addition a significant Treatment X Strain interaction was due to the larger defecation numbers displayed by the taurine-injected MR rats relative to the saline-injected MR rats.", "contents": "Long-term effects of preweaning taurine injections in rats: interactions with strain and gender. A comparison was made of MR, MNR, and RCA strains that had been injected with either 62.5 mg/kg of taurine or comparable volumes of physiological saline between postnatal Days 8 and 20 and later tested at 100 days of age in an open field and 1-way shock avoidance situations. Taurine-injected rats displayed significant elevation in defecation score in the open field. In addition a significant Treatment X Strain interaction was due to the larger defecation numbers displayed by the taurine-injected MR rats relative to the saline-injected MR rats."}
{"id": "PMID:488535", "title": "Ontogenesis of human brainstem evoked potential amplitude.", "content": "Brainstem evoked potentials (BEP's) were studied in human subjects ranging in age from birth to adulthood. The BEP's were recorded from the surface of the scalp referenced to either ear while brief clicks were delivered binaurally through earphones. Separate analyses of variance were computed for the peak-to-peak amplitude of each component of the BEP. Significant age effects were observed documenting the strong relationship between human brainstem development and amplitude changes in the BEP. The independent maturation of these structures was reflected by the significant interaction between age and peaks. Despite small fluctuations in the maturational curve of each peak, strong linear and quadratic trends were apparent. The research presented here provides evidence of significant amplitude changes in the BEP accompanying human development. These findings indicate that brainstem maturation is not yet complete at birth and that the components believed to represent spatially separate generators in the auditory system show distinct maturational stages.", "contents": "Ontogenesis of human brainstem evoked potential amplitude. Brainstem evoked potentials (BEP's) were studied in human subjects ranging in age from birth to adulthood. The BEP's were recorded from the surface of the scalp referenced to either ear while brief clicks were delivered binaurally through earphones. Separate analyses of variance were computed for the peak-to-peak amplitude of each component of the BEP. Significant age effects were observed documenting the strong relationship between human brainstem development and amplitude changes in the BEP. The independent maturation of these structures was reflected by the significant interaction between age and peaks. Despite small fluctuations in the maturational curve of each peak, strong linear and quadratic trends were apparent. The research presented here provides evidence of significant amplitude changes in the BEP accompanying human development. These findings indicate that brainstem maturation is not yet complete at birth and that the components believed to represent spatially separate generators in the auditory system show distinct maturational stages."}
{"id": "PMID:488536", "title": "Ontogenetic study of the Miwh gene in mice.", "content": "Ontogenetic and adult behavioral tests were conducted on an extreme non-agouti strain of mice carrying the Miwh mutation. Except for startle response, no significant differences were seen in any aspect of the preweaning reflex and neuromuscular tests. The lack of startle response seen in Miwh/Miwh and Miwh/+ mice can be attributed to abnormalities found in the inner ear of these genotypes. Among adult mice, significant differences were found in the open field and visual cliff tests.", "contents": "Ontogenetic study of the Miwh gene in mice. Ontogenetic and adult behavioral tests were conducted on an extreme non-agouti strain of mice carrying the Miwh mutation. Except for startle response, no significant differences were seen in any aspect of the preweaning reflex and neuromuscular tests. The lack of startle response seen in Miwh/Miwh and Miwh/+ mice can be attributed to abnormalities found in the inner ear of these genotypes. Among adult mice, significant differences were found in the open field and visual cliff tests."}
{"id": "PMID:488537", "title": "Studies of insulin binding in children using human erythrocytes in small amounts of blood.", "content": "We have demonstrated specific insulin binding by the erythrocytes (RBCs) of children. Complete binding studies were done using as little as 5 ml of blood. The receptors exhibited competition-inhibition curves and nonlinear Scatchard plots similar to those reported for insulin target tissues, such as the hepatocyte and the adipocyte. Compared with those from adults, the RBCs from children had significantly greater numbers of insulin receptors per cell (P less than 0.05). The total insulin bound by the RBCs from both children and adults, however, was not different over the physiologic range of insulin concentrations. Cord blood RBCs showed greater numbers of receptors per cell than did those from either children or adults; however, the affinity for insulin was similar in both groups. The total amount of insulin bound by cord blood was significantly greater than that in either children (P less than 0.01) or adults (P less than 0.05) over the physiologic range of insulin concentrations. The method used to measure insulin binding by erythrocytes and relatively little intra- and interassay variability, and there was little diurnal variation in binding. Storage of heparinized blood at 4 degree C for 24--36 h had no effect on insulin binding by the RBCs. We conclude that the measurement of insulin binding by RBCs from small volumes of blood may be particularly useful in the study of infants and children with disorders of carbohydrate metabolism to elucidate the role, if any, of abnormal receptor function in their condition.", "contents": "Studies of insulin binding in children using human erythrocytes in small amounts of blood. We have demonstrated specific insulin binding by the erythrocytes (RBCs) of children. Complete binding studies were done using as little as 5 ml of blood. The receptors exhibited competition-inhibition curves and nonlinear Scatchard plots similar to those reported for insulin target tissues, such as the hepatocyte and the adipocyte. Compared with those from adults, the RBCs from children had significantly greater numbers of insulin receptors per cell (P less than 0.05). The total insulin bound by the RBCs from both children and adults, however, was not different over the physiologic range of insulin concentrations. Cord blood RBCs showed greater numbers of receptors per cell than did those from either children or adults; however, the affinity for insulin was similar in both groups. The total amount of insulin bound by cord blood was significantly greater than that in either children (P less than 0.01) or adults (P less than 0.05) over the physiologic range of insulin concentrations. The method used to measure insulin binding by erythrocytes and relatively little intra- and interassay variability, and there was little diurnal variation in binding. Storage of heparinized blood at 4 degree C for 24--36 h had no effect on insulin binding by the RBCs. We conclude that the measurement of insulin binding by RBCs from small volumes of blood may be particularly useful in the study of infants and children with disorders of carbohydrate metabolism to elucidate the role, if any, of abnormal receptor function in their condition."}
{"id": "PMID:488538", "title": "Serum protein-bound hexose in diabetes: the effect of glycemic control.", "content": "To determine whether the carbohydrate content of serum proteins is related to overall glycemic control, we studied serum protein-bound hexose and glycosylated hemoglobin [HbA1(a+b+c)] in 37 ambulant diabetic patients and 32 nondiabetic controls. Protein-bound hexose was correlated with HbA1(a+b+c) in the diabetic patients (r = 0.36, P less than 0.025). The mean protein-bound hexose level of the diabetic patients was greater than that of the controls (190.8 versus 174.7 mg/dl, P less than 0.01), but diabetic patients with HbA1(a+b+c) less than 12% had a mean protein-bound hexose similar to the controls. In nine of the diabetic patients, mean protein-bound hexose and HbA1(a+b+c) were significantly reduced during a period of intensive outpatient care, while two major serum glycoproteins, haptoglobins and alpha-1-antitrypsin, were unchanged. Our findings support the hypothesis that increased glycosylation of serum proteins may occur in diabetes mellitus; this abnormality in serum protein-bound hexose may be corrected by close attention to overall glycemic control.", "contents": "Serum protein-bound hexose in diabetes: the effect of glycemic control. To determine whether the carbohydrate content of serum proteins is related to overall glycemic control, we studied serum protein-bound hexose and glycosylated hemoglobin [HbA1(a+b+c)] in 37 ambulant diabetic patients and 32 nondiabetic controls. Protein-bound hexose was correlated with HbA1(a+b+c) in the diabetic patients (r = 0.36, P less than 0.025). The mean protein-bound hexose level of the diabetic patients was greater than that of the controls (190.8 versus 174.7 mg/dl, P less than 0.01), but diabetic patients with HbA1(a+b+c) less than 12% had a mean protein-bound hexose similar to the controls. In nine of the diabetic patients, mean protein-bound hexose and HbA1(a+b+c) were significantly reduced during a period of intensive outpatient care, while two major serum glycoproteins, haptoglobins and alpha-1-antitrypsin, were unchanged. Our findings support the hypothesis that increased glycosylation of serum proteins may occur in diabetes mellitus; this abnormality in serum protein-bound hexose may be corrected by close attention to overall glycemic control."}
{"id": "PMID:488539", "title": "Nonenzymatic glucosylation of serum proteins in diabetes mellitus.", "content": "The extent of nonenzymatic glucosylation of serum protein in control and diabetic subjects was measured by a chemical procedure using thiobarbituric acid. A mean value of 0.81 (+/- 0.21 SD) nmol glucose per milligram serum protein was observed in the control group. Diabetics displayed elevated levels of glucosylated serum proteins, up to 4 nmol glucose per milligram protein. Glucosylation of serum protein correlated strongly with fasting blood sugar (r = 0.71), percent hemoglobin A1 (r = 0.79), and percent glucosylated albumin (r = 0.99). There was no overlap between control and diabetic groups, i.e., within 3 SD of the mean of controls. These studies indicate that the assay for glucosylated serum protein appears to be an especially sensitive indicator of the degree of hyperglycemia in diabetes.", "contents": "Nonenzymatic glucosylation of serum proteins in diabetes mellitus. The extent of nonenzymatic glucosylation of serum protein in control and diabetic subjects was measured by a chemical procedure using thiobarbituric acid. A mean value of 0.81 (+/- 0.21 SD) nmol glucose per milligram serum protein was observed in the control group. Diabetics displayed elevated levels of glucosylated serum proteins, up to 4 nmol glucose per milligram protein. Glucosylation of serum protein correlated strongly with fasting blood sugar (r = 0.71), percent hemoglobin A1 (r = 0.79), and percent glucosylated albumin (r = 0.99). There was no overlap between control and diabetic groups, i.e., within 3 SD of the mean of controls. These studies indicate that the assay for glucosylated serum protein appears to be an especially sensitive indicator of the degree of hyperglycemia in diabetes."}
{"id": "PMID:488541", "title": "Effect of acetate on hypoglycemic seizures in mice.", "content": "In order to determine the effects of acetate on signs and symptoms of hypoglycemic seizures, Swiss Webster albino mice were injected intraperitoneally with solutions of NaCl, NaHCO3, NH4Cl, Na-acetate, or NH4-acetate, followed by subcutaneous injection of 7 U of insulin/kg body wt. Administration of Na- or NH4-acetate delayed and reduced the incidence of hypoglycemic reactions. Reinjection with Na-acetate or repeated injections with NH4-acetate caused a return to normal behavior patterns for 60 and 75%, respectively, of the affected hypoglycemic experimental animals. Injections of control animals with NaHCO3 or NH4Cl showed that the results were not due to alkalosis or acidosis. Acetate administration significantly increased plasma acetate and citrate, but not glucose, lactate, beta-hydroxybutyrate, or acetoacetate concentrations. The results indicate that intraperitoneal administration of acetate directly acted to prevent signs of hypoglycemia from occurring and reversed its manifestations when they were present. The protective effect of acetate suggests that it may serve as a fuel for the brain.", "contents": "Effect of acetate on hypoglycemic seizures in mice. In order to determine the effects of acetate on signs and symptoms of hypoglycemic seizures, Swiss Webster albino mice were injected intraperitoneally with solutions of NaCl, NaHCO3, NH4Cl, Na-acetate, or NH4-acetate, followed by subcutaneous injection of 7 U of insulin/kg body wt. Administration of Na- or NH4-acetate delayed and reduced the incidence of hypoglycemic reactions. Reinjection with Na-acetate or repeated injections with NH4-acetate caused a return to normal behavior patterns for 60 and 75%, respectively, of the affected hypoglycemic experimental animals. Injections of control animals with NaHCO3 or NH4Cl showed that the results were not due to alkalosis or acidosis. Acetate administration significantly increased plasma acetate and citrate, but not glucose, lactate, beta-hydroxybutyrate, or acetoacetate concentrations. The results indicate that intraperitoneal administration of acetate directly acted to prevent signs of hypoglycemia from occurring and reversed its manifestations when they were present. The protective effect of acetate suggests that it may serve as a fuel for the brain."}
{"id": "PMID:488543", "title": "Spontaneous diabetes in the gnotobiotic BB/W rat.", "content": "To determine the influence of infectious agents on the initiation of diabetes in the spontaneously diabetic Bio-Breeding/Worcester (BB/W) rat, susceptible rats were raised in a germ-free environment. Between 2 and 3 mo of age, 3 of 12 pups became diabetic. Histologic examination of the pancreas revealed insulitis or end-stage islets. Culture and smears from various tissues were negative for bacteria or parasites. Serum vital antibody titers for all known rat viruses were undetectable. These data suggest that the diabetic syndrome of the BB/W rat is not dependent on recognized infectious agents.", "contents": "Spontaneous diabetes in the gnotobiotic BB/W rat. To determine the influence of infectious agents on the initiation of diabetes in the spontaneously diabetic Bio-Breeding/Worcester (BB/W) rat, susceptible rats were raised in a germ-free environment. Between 2 and 3 mo of age, 3 of 12 pups became diabetic. Histologic examination of the pancreas revealed insulitis or end-stage islets. Culture and smears from various tissues were negative for bacteria or parasites. Serum vital antibody titers for all known rat viruses were undetectable. These data suggest that the diabetic syndrome of the BB/W rat is not dependent on recognized infectious agents."}
{"id": "PMID:488544", "title": "Changes in glucagon levels after four to five weeks of glucoregulation by portable insulin infusion pumps.", "content": "Near-normal glucoregulation was maintained in five patients with juvenile-onset diabetes mellitus for 4--5 wk with a preprogrammed, continuous, subcutaneous insulin infusion using a portable battery-powered infusion pump. This form of therapy significantly lowered immunoreactive glucagon (IRG) levels below those observed while on conventional insulin treatment at several times during the 24-h profile. The maximum IRG levels were also reduced in all five subjects. Thus, a flexible system of insulin delivery, as is provided by certain open-loop pump systems, can overcome inappropriate glucagon secretion that occurs with conventional insulin therapy.", "contents": "Changes in glucagon levels after four to five weeks of glucoregulation by portable insulin infusion pumps. Near-normal glucoregulation was maintained in five patients with juvenile-onset diabetes mellitus for 4--5 wk with a preprogrammed, continuous, subcutaneous insulin infusion using a portable battery-powered infusion pump. This form of therapy significantly lowered immunoreactive glucagon (IRG) levels below those observed while on conventional insulin treatment at several times during the 24-h profile. The maximum IRG levels were also reduced in all five subjects. Thus, a flexible system of insulin delivery, as is provided by certain open-loop pump systems, can overcome inappropriate glucagon secretion that occurs with conventional insulin therapy."}
{"id": "PMID:488545", "title": "Decreased sympathetic vasomotor tone in diabetic orthostatic hypotension.", "content": "In normals, subcutaneous blood flow in the ankle region, measured by means of the 133Xe washout technique, decreases about 45% when the position of the ankle is changed from cardiac level to 50 cm below the heart. A sympathetic vascular axon reflex is responsible for this flow reduction. A normal response [mean blood-flow reduction, 49% (range, 40 to 64%)] was found in seven diabetics without neuropathy. In nine diabetics with autonomic neuropathy (beat-to-beat variation in heart rate less than or equal to 15 min-1 during hyperventilation), the reduction in blood flow on lowering of the ankle was also normal. A significant deterioration of the response was found in six diabetics with autonomic neuropathy, including orthostatic hypotension, in whom blood flow increased 23% (range, -35 to +77%). Autoregulation of blood flow, i.e., constancy of blood flow in the ankle region on lowering and elevating of the ankle 20 cm below and above cardiac level was intact in the patients with orthostatic hypotension, indicating a normal vascular smooth muscle function. In conclusion, in diabetic patients with orthostatic hypotension, the subcutaneous axon reflex is absent. This may be one of several factors responsible for their orthostatic hypotension.", "contents": "Decreased sympathetic vasomotor tone in diabetic orthostatic hypotension. In normals, subcutaneous blood flow in the ankle region, measured by means of the 133Xe washout technique, decreases about 45% when the position of the ankle is changed from cardiac level to 50 cm below the heart. A sympathetic vascular axon reflex is responsible for this flow reduction. A normal response [mean blood-flow reduction, 49% (range, 40 to 64%)] was found in seven diabetics without neuropathy. In nine diabetics with autonomic neuropathy (beat-to-beat variation in heart rate less than or equal to 15 min-1 during hyperventilation), the reduction in blood flow on lowering of the ankle was also normal. A significant deterioration of the response was found in six diabetics with autonomic neuropathy, including orthostatic hypotension, in whom blood flow increased 23% (range, -35 to +77%). Autoregulation of blood flow, i.e., constancy of blood flow in the ankle region on lowering and elevating of the ankle 20 cm below and above cardiac level was intact in the patients with orthostatic hypotension, indicating a normal vascular smooth muscle function. In conclusion, in diabetic patients with orthostatic hypotension, the subcutaneous axon reflex is absent. This may be one of several factors responsible for their orthostatic hypotension."}
{"id": "PMID:488547", "title": "A simplified method using somatostatin to assess in vivo insulin resistance over a range of obesity.", "content": "Twenty-one nondiabetic subjects, their weights ranging from 56 to 165 kg, received an infusion of glucose (420 mg/min), insulin (0.77 mU/kg/min), and somatostatin (500 microgram/h) for 150 min. A steady state level of plasma insulin and glucose was attained after 90 min. Endogenous insulin secretion determined by C-peptide measurement, and glucagon secretion remained suppressed throughout the period. With similar steady state levels of plasma insulin (SSPI) maintained in all subjects, the height of the steady state plasma glucose concentration (SSPG) was considered an index of total body sensitivity to insulin-mediated glucose uptake. A positive correlation between SSPG and the degree of obesity, as determined by the body mass index (BMI), was demonstrated (r = 0.70, P less than 0.001). No correlation was found between SSPI and BMI. The fasting plasma insulin concentration correlated with BMI (r = 0.82, P less than 0.0001) and SSPG (r = 0.80, P less than 0.0001). This method provides a simple safe measure of total body insulin resistance over a wide range of obesity and is independent of endogenous insulin secretion.", "contents": "A simplified method using somatostatin to assess in vivo insulin resistance over a range of obesity. Twenty-one nondiabetic subjects, their weights ranging from 56 to 165 kg, received an infusion of glucose (420 mg/min), insulin (0.77 mU/kg/min), and somatostatin (500 microgram/h) for 150 min. A steady state level of plasma insulin and glucose was attained after 90 min. Endogenous insulin secretion determined by C-peptide measurement, and glucagon secretion remained suppressed throughout the period. With similar steady state levels of plasma insulin (SSPI) maintained in all subjects, the height of the steady state plasma glucose concentration (SSPG) was considered an index of total body sensitivity to insulin-mediated glucose uptake. A positive correlation between SSPG and the degree of obesity, as determined by the body mass index (BMI), was demonstrated (r = 0.70, P less than 0.001). No correlation was found between SSPI and BMI. The fasting plasma insulin concentration correlated with BMI (r = 0.82, P less than 0.0001) and SSPG (r = 0.80, P less than 0.0001). This method provides a simple safe measure of total body insulin resistance over a wide range of obesity and is independent of endogenous insulin secretion."}
{"id": "PMID:488548", "title": "Bimodality of glycosylated hemoglobin distribution in Pima Indians: relationship to fasting hyperglycemia.", "content": "Glycosylated hemoglobin (HbA1) concentrations were determined in 300 Pima Indians aged 15 yr and older. Frequency distributions of HbA1 were unimodal in the 15--24-yr-old age group, but were bimodal in those aged 25 yr and over. The bimodality indicated that the subpopulation with diabetes could be identified by the presence of elevated HbA1 levels. This group was comprised primarily of subjects who also had fasting plasma glucose levels of less than or equal to 140 mg/dl, but subjects with impaired glucose tolerance without fasting hyperglycemia had HbA1 levels that were not significantly higher than those with normal glucose tolerance. The prevalence of diabetic retinopathy was much higher in the subgroup with elevated HbA1 levels and increased with increasing HbA1 level. HbA1 levels and triglyceride concentrations showed only a modest association. HbA1 determinations provided no advantage over fasting or post challenge glucose levels in the diagnosis of diabetes.", "contents": "Bimodality of glycosylated hemoglobin distribution in Pima Indians: relationship to fasting hyperglycemia. Glycosylated hemoglobin (HbA1) concentrations were determined in 300 Pima Indians aged 15 yr and older. Frequency distributions of HbA1 were unimodal in the 15--24-yr-old age group, but were bimodal in those aged 25 yr and over. The bimodality indicated that the subpopulation with diabetes could be identified by the presence of elevated HbA1 levels. This group was comprised primarily of subjects who also had fasting plasma glucose levels of less than or equal to 140 mg/dl, but subjects with impaired glucose tolerance without fasting hyperglycemia had HbA1 levels that were not significantly higher than those with normal glucose tolerance. The prevalence of diabetic retinopathy was much higher in the subgroup with elevated HbA1 levels and increased with increasing HbA1 level. HbA1 levels and triglyceride concentrations showed only a modest association. HbA1 determinations provided no advantage over fasting or post challenge glucose levels in the diagnosis of diabetes."}
{"id": "PMID:488549", "title": "The effects of different conditions of organ culture on the survival of the mouse pancreas.", "content": "The effects of different conditions of organ culture on the viability of murine pancreatic tissues and, particularly, of pancreatic islets, was assessed by morphologic and histochemical analyses. High oxygen tension (1300 mm Hg), found previously to allow successful allogeneic thyroid transplantation, was toxic to pancreatic tissue. Low temperature (22 degrees C) was associated with better islet preservation than high temperature (37 degrees C). Hydrocortisone did not reduce the survival of pancreatic islets and did appear to preserve the acinar components. Fetal and neonatal pancreas responded similarly to the various organ culture conditions. These results have potential importance in guiding future transplantation experiments.", "contents": "The effects of different conditions of organ culture on the survival of the mouse pancreas. The effects of different conditions of organ culture on the viability of murine pancreatic tissues and, particularly, of pancreatic islets, was assessed by morphologic and histochemical analyses. High oxygen tension (1300 mm Hg), found previously to allow successful allogeneic thyroid transplantation, was toxic to pancreatic tissue. Low temperature (22 degrees C) was associated with better islet preservation than high temperature (37 degrees C). Hydrocortisone did not reduce the survival of pancreatic islets and did appear to preserve the acinar components. Fetal and neonatal pancreas responded similarly to the various organ culture conditions. These results have potential importance in guiding future transplantation experiments."}
{"id": "PMID:488550", "title": "Changes in glycoproteins of liver plasma membranes from rats treated with D-galactosamine.", "content": "D-Galactosamine administration to rats (400 mg/kg) by intraperitoneal injection induced biochemical alterations in liver plasma membranes. Alterations were studied 4, 16 and 24 h after D-galactosamine injection. Plasma membrane 5'-mononucleotidase activity decreased to 40% of control values. Carbohydrate composition was significantly changed. After 24 h D-galactosamine administration, the diminution in plasma membrane sialic acids and hexoses reached 30% of control values. As detected by SDS-acrylamide gel electrophoresis, high molecular weight glycoproteins of D-galactosamine-treated plasma membranes were modified. Moreover, the incorporation of [35S]-sulfate into membrane glycoproteins decreased after D-galactosamine administration (40--60% of control). The present results show that biochemical alterations in rat liver plasma membranes appear soon after D-galactosamine injection. Marked changes are observed in cell surface glycoproteins, especially in sialoglycoproteins and sulfated glycoproteins.", "contents": "Changes in glycoproteins of liver plasma membranes from rats treated with D-galactosamine. D-Galactosamine administration to rats (400 mg/kg) by intraperitoneal injection induced biochemical alterations in liver plasma membranes. Alterations were studied 4, 16 and 24 h after D-galactosamine injection. Plasma membrane 5'-mononucleotidase activity decreased to 40% of control values. Carbohydrate composition was significantly changed. After 24 h D-galactosamine administration, the diminution in plasma membrane sialic acids and hexoses reached 30% of control values. As detected by SDS-acrylamide gel electrophoresis, high molecular weight glycoproteins of D-galactosamine-treated plasma membranes were modified. Moreover, the incorporation of [35S]-sulfate into membrane glycoproteins decreased after D-galactosamine administration (40--60% of control). The present results show that biochemical alterations in rat liver plasma membranes appear soon after D-galactosamine injection. Marked changes are observed in cell surface glycoproteins, especially in sialoglycoproteins and sulfated glycoproteins."}
{"id": "PMID:488551", "title": "Vagovagal stimulation of pancreatic-polypeptide secretion by graded distention of the gastric fundus and antrum in man.", "content": "Previous studies have shown that pancreatic polypeptide (PP) secretion is regulated by efferent, vagal stimulation. In the present study the afferent part of a vagovagal pathway has been investigated in two different ways: (I) Graded fundic distention: in 8 patients with duodenal ulcer ballon distention with 150 ml of the fundus and the body of the stomach increased PP concentrations in plasma from 19 (17--26) to 41 (35--48) pmol/l, median and interquartile range. Distention by 300 and 600 ml did not further increase PP concentrations. After denervation of the fundus by proximal gastric vagotomy, no increase in PP levels was observed during distention with 50 and 300 ml whereas distention by 600 ml was followed by a small increase. (II) Graded antral distention: balloon distention with 50, 100 and 150 ml of the antrum increased plasma PP concentrations in 7 healthy subjects and 14 duodenal ulcer patients. Maximal PP response was achieved by distention with 100 ml, healthy subjects: from 14 (12--23) to 40 (26--44) pmol/l, and duodenal ulcer patients: from 25 (13--38) to 47 (22--63) pmol/l, median and interquartile range. It is concluded that a gastropancreatic reflex stimulating PP secretion through a long vagovagal pathway is present in man, and that this mechanism probably is involved in the initial PP response during a meal.", "contents": "Vagovagal stimulation of pancreatic-polypeptide secretion by graded distention of the gastric fundus and antrum in man. Previous studies have shown that pancreatic polypeptide (PP) secretion is regulated by efferent, vagal stimulation. In the present study the afferent part of a vagovagal pathway has been investigated in two different ways: (I) Graded fundic distention: in 8 patients with duodenal ulcer ballon distention with 150 ml of the fundus and the body of the stomach increased PP concentrations in plasma from 19 (17--26) to 41 (35--48) pmol/l, median and interquartile range. Distention by 300 and 600 ml did not further increase PP concentrations. After denervation of the fundus by proximal gastric vagotomy, no increase in PP levels was observed during distention with 50 and 300 ml whereas distention by 600 ml was followed by a small increase. (II) Graded antral distention: balloon distention with 50, 100 and 150 ml of the antrum increased plasma PP concentrations in 7 healthy subjects and 14 duodenal ulcer patients. Maximal PP response was achieved by distention with 100 ml, healthy subjects: from 14 (12--23) to 40 (26--44) pmol/l, and duodenal ulcer patients: from 25 (13--38) to 47 (22--63) pmol/l, median and interquartile range. It is concluded that a gastropancreatic reflex stimulating PP secretion through a long vagovagal pathway is present in man, and that this mechanism probably is involved in the initial PP response during a meal."}
{"id": "PMID:488552", "title": "Inhibition of bombesin-stimulated gastric acid secretion by secretin, glucagon and caerulein in patients with duodenal ulcer.", "content": "The inhibitory effects of intravenous infusions of secretin, glucagon and caerulein on the gastric acid response to bombesin were studied in 8 duodenal ulcer patients. Bombesin was found to be a very potent stimulator of gastric acid secretion in patients with duodenal ulcer. There were no significant differences in acid outputs per 15-min period between bombesin infused in a dose of 0.9 microgram/kg/h and pentagastrin infusion administered in a maximal dose, at a rate of 6.0 microgram/kg/h. Secretin (1 U/kg/h), glucagon (30 microgram/kg/h) and caerulein (0.1 microgram/kg/h) produced significant decreases in gastric acid secretion evoked by bombesin given in a dose of 0.9 microgram/kg/h. Percentages of inhibition were 48.6, 45.2 and 35.5, respectively. It is supposed that secretin and glucagon given in pharmacological doses are capable of interfering with the action of gastrin released from antrum by means of bombesin on the parietal cell by noncompetitive kinetics. Caerulein administered in a pharmacological dosis, however, can inhibit the effect of gastrin released by bombesin on the parietal cells by a competitive kinetic.", "contents": "Inhibition of bombesin-stimulated gastric acid secretion by secretin, glucagon and caerulein in patients with duodenal ulcer. The inhibitory effects of intravenous infusions of secretin, glucagon and caerulein on the gastric acid response to bombesin were studied in 8 duodenal ulcer patients. Bombesin was found to be a very potent stimulator of gastric acid secretion in patients with duodenal ulcer. There were no significant differences in acid outputs per 15-min period between bombesin infused in a dose of 0.9 microgram/kg/h and pentagastrin infusion administered in a maximal dose, at a rate of 6.0 microgram/kg/h. Secretin (1 U/kg/h), glucagon (30 microgram/kg/h) and caerulein (0.1 microgram/kg/h) produced significant decreases in gastric acid secretion evoked by bombesin given in a dose of 0.9 microgram/kg/h. Percentages of inhibition were 48.6, 45.2 and 35.5, respectively. It is supposed that secretin and glucagon given in pharmacological doses are capable of interfering with the action of gastrin released from antrum by means of bombesin on the parietal cell by noncompetitive kinetics. Caerulein administered in a pharmacological dosis, however, can inhibit the effect of gastrin released by bombesin on the parietal cells by a competitive kinetic."}
{"id": "PMID:488553", "title": "Influence of bile on kinetic behavior of colonic epithelial cells of the rat.", "content": "The acute effect of bile deprivation on colonic epithelial cell proliferation in Sprague-Dawley rats was investigated and compared with its effect on jejunum and ileum. 2. days after the creation of bile fistula, tritiated thymidine was injected and animals sacrificed 1 and 24 h later. Compared with control untreated animals, sham-operated restrained rats had a reduced labeling and mitotic index of the colonic epithelial cell population as well as a slower migration of cells to the lumen. Colonic cell proliferation in animals deprived of bile flow was reduced a further 50%. Moreover, no evidence of cell migration or appreciable decline in grain density was seen over 24 h in bile fistula rats. Alterations in cell proliferation in both sham and bile fistula treated rats became less marked as one proceeded proximally to the small bowel. Therefore, significant alterations in cell kinetics result when normal bile flow is interrupted, suggesting its importance in the regulatory control of colonic cell proliferation.", "contents": "Influence of bile on kinetic behavior of colonic epithelial cells of the rat. The acute effect of bile deprivation on colonic epithelial cell proliferation in Sprague-Dawley rats was investigated and compared with its effect on jejunum and ileum. 2. days after the creation of bile fistula, tritiated thymidine was injected and animals sacrificed 1 and 24 h later. Compared with control untreated animals, sham-operated restrained rats had a reduced labeling and mitotic index of the colonic epithelial cell population as well as a slower migration of cells to the lumen. Colonic cell proliferation in animals deprived of bile flow was reduced a further 50%. Moreover, no evidence of cell migration or appreciable decline in grain density was seen over 24 h in bile fistula rats. Alterations in cell proliferation in both sham and bile fistula treated rats became less marked as one proceeded proximally to the small bowel. Therefore, significant alterations in cell kinetics result when normal bile flow is interrupted, suggesting its importance in the regulatory control of colonic cell proliferation."}
{"id": "PMID:488554", "title": "Effect of an elemental diet (Vivonex) on the absorption abnormalities and histological appearances of the jejunum in untreated adult coeliac disease.", "content": "The effect of an elemental diet (Vivonex) together with a gluten-free diet on the absorption of water, sodium and chloride in the jejunum was studied in 4 patients with untreated adult coeliac disease before and after a 1-month course of therapy. The morphology of the jejunum was also studied by jejunal biopsy taken at the same time as the intestinal perfusion. The results were compared with those obtained in 4 patients with adult coeliac disease treated with a gluten-free diet alone. No marked improvement was noted in the transportation of water, sodium and chloride after either treatment with Vivonex and a gluten-free diet or after a gluten-free diet alone, and no marked histological changes were found. Clinical improvement occurred in both groups of patients, in that the diarrhoea improved in all patients and they generally felt better. There appears to be no additional advantage of using an elemental diet with a gluten-free diet in the initial management of adult coeliac disease.", "contents": "Effect of an elemental diet (Vivonex) on the absorption abnormalities and histological appearances of the jejunum in untreated adult coeliac disease. The effect of an elemental diet (Vivonex) together with a gluten-free diet on the absorption of water, sodium and chloride in the jejunum was studied in 4 patients with untreated adult coeliac disease before and after a 1-month course of therapy. The morphology of the jejunum was also studied by jejunal biopsy taken at the same time as the intestinal perfusion. The results were compared with those obtained in 4 patients with adult coeliac disease treated with a gluten-free diet alone. No marked improvement was noted in the transportation of water, sodium and chloride after either treatment with Vivonex and a gluten-free diet or after a gluten-free diet alone, and no marked histological changes were found. Clinical improvement occurred in both groups of patients, in that the diarrhoea improved in all patients and they generally felt better. There appears to be no additional advantage of using an elemental diet with a gluten-free diet in the initial management of adult coeliac disease."}
{"id": "PMID:488555", "title": "Cimetidine and salbutamol combined inhibition of gastric acid secretion in the dog.", "content": "The effects of salbutamol and cimetidine, alone and in combination, on pentagastrin-stimulated gastric secretion have been studied in conscious dogs with gastric fistulae. Salbutamol 24 nmol/kg.h alone and cimetidine 4 mumol/kg.h alone inhibited acid up to 69%, cimetidine 8 mumol/kg.h produced up to 87% inhibition and the combination of cimetidine 4 mumol/kg.h and salbutamol 24 nmol/kg.h almost totally inhibited acid (up to 99%). The pharmacokinetics of these inhibitions were studied and salbutamol and/or cimetidine caused a complex uncompetitive inhibition of pentagastrin-stimulated acid. The inhibitory combination of the two drugs was additive.", "contents": "Cimetidine and salbutamol combined inhibition of gastric acid secretion in the dog. The effects of salbutamol and cimetidine, alone and in combination, on pentagastrin-stimulated gastric secretion have been studied in conscious dogs with gastric fistulae. Salbutamol 24 nmol/kg.h alone and cimetidine 4 mumol/kg.h alone inhibited acid up to 69%, cimetidine 8 mumol/kg.h produced up to 87% inhibition and the combination of cimetidine 4 mumol/kg.h and salbutamol 24 nmol/kg.h almost totally inhibited acid (up to 99%). The pharmacokinetics of these inhibitions were studied and salbutamol and/or cimetidine caused a complex uncompetitive inhibition of pentagastrin-stimulated acid. The inhibitory combination of the two drugs was additive."}
{"id": "PMID:488556", "title": "Lysosomal changes and enterocytic copper deposits in Wilson's disease.", "content": "In 4 siblings, of whom 2 had symptoms of Wilson's disease, electron microscopy showed that the number and size of lysosomes in the enterocytes were increased compared with controls. Electron-dense aggregates, observed near the lysosomes in the 2 symptomatic patients and 1 other sibling, were shown, by means of a microanalytical method, to contain copper. These findings could suggest a possible role of the small intestine in Wilson's disease.", "contents": "Lysosomal changes and enterocytic copper deposits in Wilson's disease. In 4 siblings, of whom 2 had symptoms of Wilson's disease, electron microscopy showed that the number and size of lysosomes in the enterocytes were increased compared with controls. Electron-dense aggregates, observed near the lysosomes in the 2 symptomatic patients and 1 other sibling, were shown, by means of a microanalytical method, to contain copper. These findings could suggest a possible role of the small intestine in Wilson's disease."}
{"id": "PMID:488565", "title": "Reduced insulin binding to hepatic plasma membranes in D-galactosamine-treated rats.", "content": "Six to 12 hr after IP injection of 400 mg/kg of D-galactosamine in rats a 5-fold increase in plasma insulin was observed. In addition, impaired glucose assimilation was present after an IV Load in spite of unchanged fasting glucose levels. In streptozotocin-diabetic rats (100 mg/kg IV) plasma insulin remained diminished 12 h after induction of D-galactosamine hepatitis. Under identical conditions of preparation and incubation, the liver plasma membranes of D-galactosamine-treated rats, in both normal and diabetic states, bound only 40--60% as much insulin per mg of membrane protein as those of the control rats. Scatchard analysis suggested that this was due to a decrease in the number of receptor sites in the membranes of the D-galactosamine-injected rats. No difference in the insulin degrading capacity and in insulin-receptor dissociation of the plasma membranes between control and D-galactosamine-treated groups was found. These data suggest that a reduction in the number of hepatic insulin receptors in galactosamine hepatitis can lead to insulin resistance and hyperinsulinaemia.", "contents": "Reduced insulin binding to hepatic plasma membranes in D-galactosamine-treated rats. Six to 12 hr after IP injection of 400 mg/kg of D-galactosamine in rats a 5-fold increase in plasma insulin was observed. In addition, impaired glucose assimilation was present after an IV Load in spite of unchanged fasting glucose levels. In streptozotocin-diabetic rats (100 mg/kg IV) plasma insulin remained diminished 12 h after induction of D-galactosamine hepatitis. Under identical conditions of preparation and incubation, the liver plasma membranes of D-galactosamine-treated rats, in both normal and diabetic states, bound only 40--60% as much insulin per mg of membrane protein as those of the control rats. Scatchard analysis suggested that this was due to a decrease in the number of receptor sites in the membranes of the D-galactosamine-injected rats. No difference in the insulin degrading capacity and in insulin-receptor dissociation of the plasma membranes between control and D-galactosamine-treated groups was found. These data suggest that a reduction in the number of hepatic insulin receptors in galactosamine hepatitis can lead to insulin resistance and hyperinsulinaemia."}
{"id": "PMID:488566", "title": "Effect of experimental hyperinsulinaemia on intracellular glucose metabolism of isolated adipocytes.", "content": "Glucose oxidation and lipogenesis were studied in isolated adipocytes from control and non-obese, experimentally hyperinsulinaemic rats. In cells from the hyperinsulinaemic animals oxidation of either [1(-14) C]- or [6(-14) C] glucose was increased in the presence or absence of insulin, at substrate concentrations from 0.1 to 20 mmol/l. Glucose incorporation into total triglycerides and fatty acids was also increased. These enhanced rates of glucose metabolism were due to increased activity of the glucose transport system in addition to increased activity of intracellular glucose metabolism. Therefore, these data indicate that insulin can influence long term glucose homoeostasis by augmenting the overall cellular capacity for glucose metabolism at several loci.", "contents": "Effect of experimental hyperinsulinaemia on intracellular glucose metabolism of isolated adipocytes. Glucose oxidation and lipogenesis were studied in isolated adipocytes from control and non-obese, experimentally hyperinsulinaemic rats. In cells from the hyperinsulinaemic animals oxidation of either [1(-14) C]- or [6(-14) C] glucose was increased in the presence or absence of insulin, at substrate concentrations from 0.1 to 20 mmol/l. Glucose incorporation into total triglycerides and fatty acids was also increased. These enhanced rates of glucose metabolism were due to increased activity of the glucose transport system in addition to increased activity of intracellular glucose metabolism. Therefore, these data indicate that insulin can influence long term glucose homoeostasis by augmenting the overall cellular capacity for glucose metabolism at several loci."}
{"id": "PMID:488570", "title": "The effect of unabsorbable carbohydrate on gut hormones. Modification of post-prandial GIP secretion by guar.", "content": "Five healthy volunteers and 6 diabetics were given a mixed test meal on two occasions--once with and once without 10 g guar flour. Addition of guar caused a 47% decrease in maximum post-prandial GIP levels, a 48% decrease in blood glucose and a 48% decrease in plasma insulin in normal subjects. In diabetics, addition of guar caused a 30% reduction in maximum post-prandial GIP and 58% decrease in blood glucose. Four normal and 6 diabetic subjects were given a predominantly carbohydrate meal, again with and without 10 g guar. Addition of guar caused a 78% decrease in blood glucose and a 59% decrease in plasma insulin in normal subjects. In diabetics addition of guar caused a 71% decrease in maximum post-prandial plasma GIP and a 68% decrease in blood glucose. Lowering of post-prandial blood glucose, plasma insulin and GIP levels by guar was statistically significant in every case. Addition of guar to the predominantly carbohydrate meal caused a decrease in total plasma GLI in both normal and diabetic subjects but reached statistical significance only in the normal subjects. There was a highly significant correlation (r = 0.83; p less than 0.0005) between peak post-prandial insulin levels in normal subjects and the corresponding plasma GIP concentration. The reduction of GIP or GLI secretion may, therefore, be partly responsible for the smaller rise in plasma insulin observed in normal volunteers when guar is added to meals.", "contents": "The effect of unabsorbable carbohydrate on gut hormones. Modification of post-prandial GIP secretion by guar. Five healthy volunteers and 6 diabetics were given a mixed test meal on two occasions--once with and once without 10 g guar flour. Addition of guar caused a 47% decrease in maximum post-prandial GIP levels, a 48% decrease in blood glucose and a 48% decrease in plasma insulin in normal subjects. In diabetics, addition of guar caused a 30% reduction in maximum post-prandial GIP and 58% decrease in blood glucose. Four normal and 6 diabetic subjects were given a predominantly carbohydrate meal, again with and without 10 g guar. Addition of guar caused a 78% decrease in blood glucose and a 59% decrease in plasma insulin in normal subjects. In diabetics addition of guar caused a 71% decrease in maximum post-prandial plasma GIP and a 68% decrease in blood glucose. Lowering of post-prandial blood glucose, plasma insulin and GIP levels by guar was statistically significant in every case. Addition of guar to the predominantly carbohydrate meal caused a decrease in total plasma GLI in both normal and diabetic subjects but reached statistical significance only in the normal subjects. There was a highly significant correlation (r = 0.83; p less than 0.0005) between peak post-prandial insulin levels in normal subjects and the corresponding plasma GIP concentration. The reduction of GIP or GLI secretion may, therefore, be partly responsible for the smaller rise in plasma insulin observed in normal volunteers when guar is added to meals."}
{"id": "PMID:488571", "title": "Basement membrane thickness, insulin antibodies and HLA-antigens in long standing insulin dependent diabetics with and without severe retinopathy.", "content": "The study was designed to show whether there was any relation between muscle capillary basement membrane thickness, HLA-antigens, anti-insulin antibodies and proliferative retinopathy. Electron microscopic measurements of muscle capillary basement membrane thickness were performed on muscle biopsies from 15 insulin-dependent diabetics and severe proliferative retinopathy, 24 insulin-dependent diabetics with minimal retinopathy and 18 age- and sex matched non-diabetics. All the patients had had diabetes for 20 years or more. None had biochemical or clinical evidence of diabetic nephropathy. Basement membrane thickness was measured according to the methods of Siperstein and Williamson. Muscle capillary basement membrane thickening occurred in 32 of 39 diabetics, using the Siperstein method, but patients with proliferative retinopathy did not exhibit thicker basement membranes than patients with no or minimal changes in the retina. There were apparent differences in HLA-antigens between diabetics with and without proliferative retinopathy, but they did not reach statistical significance. There was no correlation between muscle capillary basement membrane thickness and the quantity of insulin antibodies. The results indicate that factors other than basement membrane thickening and genetic factors in the HLA-region, are responsible for the development of proliferative retinopathy.", "contents": "Basement membrane thickness, insulin antibodies and HLA-antigens in long standing insulin dependent diabetics with and without severe retinopathy. The study was designed to show whether there was any relation between muscle capillary basement membrane thickness, HLA-antigens, anti-insulin antibodies and proliferative retinopathy. Electron microscopic measurements of muscle capillary basement membrane thickness were performed on muscle biopsies from 15 insulin-dependent diabetics and severe proliferative retinopathy, 24 insulin-dependent diabetics with minimal retinopathy and 18 age- and sex matched non-diabetics. All the patients had had diabetes for 20 years or more. None had biochemical or clinical evidence of diabetic nephropathy. Basement membrane thickness was measured according to the methods of Siperstein and Williamson. Muscle capillary basement membrane thickening occurred in 32 of 39 diabetics, using the Siperstein method, but patients with proliferative retinopathy did not exhibit thicker basement membranes than patients with no or minimal changes in the retina. There were apparent differences in HLA-antigens between diabetics with and without proliferative retinopathy, but they did not reach statistical significance. There was no correlation between muscle capillary basement membrane thickness and the quantity of insulin antibodies. The results indicate that factors other than basement membrane thickening and genetic factors in the HLA-region, are responsible for the development of proliferative retinopathy."}
{"id": "PMID:488572", "title": "Absorption kinetics of subcutaneously injected insulin. Evidence for degradation at the injection site.", "content": "The absorption of subcutaneously injected insulin was examined by injecting semisynthetic [3H] insulin in anaesthetized pigs and subsequently analysing the tissue excised from the injection site. Contrary to previously accepted views, a significant proportion of insulin was degraded at the injection site. The disappearance of intact [3H] insulin from the injection site followed a monoexponential function with a half-time of 59 min.", "contents": "Absorption kinetics of subcutaneously injected insulin. Evidence for degradation at the injection site. The absorption of subcutaneously injected insulin was examined by injecting semisynthetic [3H] insulin in anaesthetized pigs and subsequently analysing the tissue excised from the injection site. Contrary to previously accepted views, a significant proportion of insulin was degraded at the injection site. The disappearance of intact [3H] insulin from the injection site followed a monoexponential function with a half-time of 59 min."}
{"id": "PMID:488573", "title": "[Statistical findings on the variability of chemo-antibiotic resistance in minor Salmonella strains particularly frequent in the Piedmont area].", "content": "462 Strains of Salmonella spp. were tested; they had been arranged in species according to the frequency of appearance in Regione Piemonte: 358 of them were isolated in the period 1975-'76 and 104 in 1977. The susceptibility of the two groups considered to 40 antibiotics was determined. The results obtained evidenced significant variations of this parameter.", "contents": "[Statistical findings on the variability of chemo-antibiotic resistance in minor Salmonella strains particularly frequent in the Piedmont area]. 462 Strains of Salmonella spp. were tested; they had been arranged in species according to the frequency of appearance in Regione Piemonte: 358 of them were isolated in the period 1975-'76 and 104 in 1977. The susceptibility of the two groups considered to 40 antibiotics was determined. The results obtained evidenced significant variations of this parameter."}
{"id": "PMID:488574", "title": "[Epidemiological aspects of salmonelloses in Turin. II: The presence of Salmonella in surface waters and sewerage waters of Turin].", "content": "The Authors have examined several sets of superficial water and of sewerage waters (respectively 44 and 27) in the metropolitan area of Turin in search of Salmonella bacteria.", "contents": "[Epidemiological aspects of salmonelloses in Turin. II: The presence of Salmonella in surface waters and sewerage waters of Turin]. The Authors have examined several sets of superficial water and of sewerage waters (respectively 44 and 27) in the metropolitan area of Turin in search of Salmonella bacteria."}
{"id": "PMID:488575", "title": "[Routes of spread of the current rabies epizoonosis in Europe].", "content": "The present report describes the spreading paths of the rabic epidemic that is rapidly reaching Italy from Central Europe. The epidemiologic situation of neighbouring countries is examined and the sanitary measures undertaken in Europe against epidemic are compared.", "contents": "[Routes of spread of the current rabies epizoonosis in Europe]. The present report describes the spreading paths of the rabic epidemic that is rapidly reaching Italy from Central Europe. The epidemiologic situation of neighbouring countries is examined and the sanitary measures undertaken in Europe against epidemic are compared."}
{"id": "PMID:488576", "title": "[Sensitivity of human and pigeon erythrocytes to rubella virus].", "content": "Human cord blood and adult red blood cells (rbc) and pigeon rbc were compared for sensitivity to rubella virus hemagglutination by a new method, consisting of the direct counting of free and aggregate red blood cells by using an hemocytometer chamber. With this method, more reliable than the \"pattern test\", hemagglutination is quantitated by the \"aggregation index\" (A.I.). Pigeon rbc were more sensitive than human rbc; cord blood and trypsinized adult human rbc showed the same sensitivity. However the kinetics of hemagglutination, evaluated by A.I. after different incubation times, were essentially the same with the three rbc types.", "contents": "[Sensitivity of human and pigeon erythrocytes to rubella virus]. Human cord blood and adult red blood cells (rbc) and pigeon rbc were compared for sensitivity to rubella virus hemagglutination by a new method, consisting of the direct counting of free and aggregate red blood cells by using an hemocytometer chamber. With this method, more reliable than the \"pattern test\", hemagglutination is quantitated by the \"aggregation index\" (A.I.). Pigeon rbc were more sensitive than human rbc; cord blood and trypsinized adult human rbc showed the same sensitivity. However the kinetics of hemagglutination, evaluated by A.I. after different incubation times, were essentially the same with the three rbc types."}
{"id": "PMID:488578", "title": "[Epidemiological aspects relating to the determination of fosfomycin sensitivity: results of a study on 300 strains isolated in a hospital milieu in Turin].", "content": "The AA. evaluated the sensitivity to Fosfomycin and to other commonly used antibiotics of 105 bacterial strains isolated from hospital environment and 195 from pathological materials. They also studied the problem of bacterial resistence to Fosfomycin considering the frequency of \"inner colonies\" and of bacterial cross-resistance to other antibiotics.", "contents": "[Epidemiological aspects relating to the determination of fosfomycin sensitivity: results of a study on 300 strains isolated in a hospital milieu in Turin]. The AA. evaluated the sensitivity to Fosfomycin and to other commonly used antibiotics of 105 bacterial strains isolated from hospital environment and 195 from pathological materials. They also studied the problem of bacterial resistence to Fosfomycin considering the frequency of \"inner colonies\" and of bacterial cross-resistance to other antibiotics."}
{"id": "PMID:488577", "title": "[HBsAG in feces, urine and saliva].", "content": "After some observations about the tests of the research exposed in the literature, authors illustrate the tests for 142 patients divided into 5 groups: a) patients affected with acute viral hepatitis; b) patients affected with praegressa acute viral hepatitis; c) relatives of patients with acute viral hepatitis; d) volunteers; e) patients affected with chronic uraemia under dialisis periodic treatment. After the testing control, authors, conclude with an hipotesis: a possible epidemic function of faeces, urine saliva, in the passage of the acute viral hepatitis.", "contents": "[HBsAG in feces, urine and saliva]. After some observations about the tests of the research exposed in the literature, authors illustrate the tests for 142 patients divided into 5 groups: a) patients affected with acute viral hepatitis; b) patients affected with praegressa acute viral hepatitis; c) relatives of patients with acute viral hepatitis; d) volunteers; e) patients affected with chronic uraemia under dialisis periodic treatment. After the testing control, authors, conclude with an hipotesis: a possible epidemic function of faeces, urine saliva, in the passage of the acute viral hepatitis."}
{"id": "PMID:488579", "title": "[Transient enterotoxemia following tooth extraction: observations on the Limulus test].", "content": "The incidence of endotoxemia (with limulus assay) in a group of patients undergoing dental extraction of both normal and diseased teeth was determined. Transient endotoxemia was found in 7 caes, without incidence of bacteremia.", "contents": "[Transient enterotoxemia following tooth extraction: observations on the Limulus test]. The incidence of endotoxemia (with limulus assay) in a group of patients undergoing dental extraction of both normal and diseased teeth was determined. Transient endotoxemia was found in 7 caes, without incidence of bacteremia."}
{"id": "PMID:488592", "title": "[Distribution and anomalies of coronary arteries in tetralogy of Fallot (author's transl)].", "content": "Anomalies in the course and distribution of coronary arteries may complicate the surgical correction of tetralogy of Fallot (TF). With the aim of preventing possible serious, and even lethal, cardiac events caused by the injury of aberrant vessels, 119 patients with TF were subjected to selective coronary angiography. Apart from demonstrating anastomoses between the coronary and bronchial arteries in 31 cases, small fistulas between the coronary arteries and pulmonary artery (in 14 cases) and hypoplasia of the entire coronary tree in 1 case, this investigation revealed anomalies in the origin of coronary branches in 11 cases. In some cases the first septal branch was absent and the diagonal branches were poorly developed. An injury to the coronary system, following the closure of the interventricular septal defect, may possibly explain the onset of a \"left anterior hemiblock\" after total correction of TF. Selective coronary angiography in small children presents no riskes provided it is correctly performed. In our cases no complications arose.", "contents": "[Distribution and anomalies of coronary arteries in tetralogy of Fallot (author's transl)]. Anomalies in the course and distribution of coronary arteries may complicate the surgical correction of tetralogy of Fallot (TF). With the aim of preventing possible serious, and even lethal, cardiac events caused by the injury of aberrant vessels, 119 patients with TF were subjected to selective coronary angiography. Apart from demonstrating anastomoses between the coronary and bronchial arteries in 31 cases, small fistulas between the coronary arteries and pulmonary artery (in 14 cases) and hypoplasia of the entire coronary tree in 1 case, this investigation revealed anomalies in the origin of coronary branches in 11 cases. In some cases the first septal branch was absent and the diagonal branches were poorly developed. An injury to the coronary system, following the closure of the interventricular septal defect, may possibly explain the onset of a \"left anterior hemiblock\" after total correction of TF. Selective coronary angiography in small children presents no riskes provided it is correctly performed. In our cases no complications arose."}
{"id": "PMID:488580", "title": "[Case of L. monocytogenes meningitis in the non-neonatal period].", "content": "A case of Listeria monocytogenes meningitidis in a little girl of Envie (Cuneo) is reported. The epidemiologic study has shown the presence of significatively high antibodies titers also in the sera of other members of the family.", "contents": "[Case of L. monocytogenes meningitis in the non-neonatal period]. A case of Listeria monocytogenes meningitidis in a little girl of Envie (Cuneo) is reported. The epidemiologic study has shown the presence of significatively high antibodies titers also in the sera of other members of the family."}
{"id": "PMID:488594", "title": "[Clinical and epidemiological criteria of evaluation of the exercise test (author's transl)].", "content": "Exercise test diagnostic and prognostic value depends on the purpose for which is performed and on the population studied. In typical angina patients the test is usefull for choosing between medical and surgical therapy and for evaluating treatment efficacy. In patients with atypical chest pain a diagnostic value is recognized. In asymptomatic subjects results of a number of epidemiological studies suggest that this test is recommended only in selected groups or for research purpose. In the appendix definitions of epidemiological measures used in screening tests evaluation are given with examples.", "contents": "[Clinical and epidemiological criteria of evaluation of the exercise test (author's transl)]. Exercise test diagnostic and prognostic value depends on the purpose for which is performed and on the population studied. In typical angina patients the test is usefull for choosing between medical and surgical therapy and for evaluating treatment efficacy. In patients with atypical chest pain a diagnostic value is recognized. In asymptomatic subjects results of a number of epidemiological studies suggest that this test is recommended only in selected groups or for research purpose. In the appendix definitions of epidemiological measures used in screening tests evaluation are given with examples."}
{"id": "PMID:488595", "title": "[Effort test on a treadmill in peripheral arteriopathic obstructions (author's transl)].", "content": "20 males, 48 to 66 of age, whose 10 normal and 10 with chronic arteriopatic obstruction at inferior limbs, were submitted to effort on a treadmill for 5 minutes or until pain. The clinostatic systolic aterial pressure was obtained in the four limbs before and at fixed intervals of time from the exercise. Arterial pressure increases after effort in the normal limbs; the length and the amount of the systolic pressure decrease after exercise are in correlation with the degree of the arterial involvement. The exercise on treadmill seems useful when the clinical and other diagnostic data are inadequate for a reliable diagnosis and to evaluate the progression of the arteriopathy through a non-invasive, and easy to repeat, method. The possible mechanisms of the pressor decrease in the arteriopatic limbs are discussed.", "contents": "[Effort test on a treadmill in peripheral arteriopathic obstructions (author's transl)]. 20 males, 48 to 66 of age, whose 10 normal and 10 with chronic arteriopatic obstruction at inferior limbs, were submitted to effort on a treadmill for 5 minutes or until pain. The clinostatic systolic aterial pressure was obtained in the four limbs before and at fixed intervals of time from the exercise. Arterial pressure increases after effort in the normal limbs; the length and the amount of the systolic pressure decrease after exercise are in correlation with the degree of the arterial involvement. The exercise on treadmill seems useful when the clinical and other diagnostic data are inadequate for a reliable diagnosis and to evaluate the progression of the arteriopathy through a non-invasive, and easy to repeat, method. The possible mechanisms of the pressor decrease in the arteriopatic limbs are discussed."}
{"id": "PMID:488597", "title": "[Left atrial myxoma revealed by saddle occlusion of the aortic carrefour (author's transl)].", "content": "A case of a left atrial myxoma revealed a saddle occlusion of aortic carrefour is reported. The embolus has been surgically removed and histology allowed to recognise its myxomatous nature, suggesting source for left atrial cavity. Cardiac catheterisation and angiocardiography confirmed the presence of atrial tumor, which has been successfully excised. Embolisation is referred to villous, friable surface of the neoplasm. Employment of echocardiography in patients with arterial emboli from unknown etiology is highly recommended.", "contents": "[Left atrial myxoma revealed by saddle occlusion of the aortic carrefour (author's transl)]. A case of a left atrial myxoma revealed a saddle occlusion of aortic carrefour is reported. The embolus has been surgically removed and histology allowed to recognise its myxomatous nature, suggesting source for left atrial cavity. Cardiac catheterisation and angiocardiography confirmed the presence of atrial tumor, which has been successfully excised. Embolisation is referred to villous, friable surface of the neoplasm. Employment of echocardiography in patients with arterial emboli from unknown etiology is highly recommended."}
{"id": "PMID:488598", "title": "[A rare case of Friedreich's atoxia with cardiac involvement: the torsade de pointe (author's transl)].", "content": "The Authors describe a case of Friedreich's ataxia complicated by an unusual arrhythmia, torsade de pointes, the only manifestation of cardiac involvment in this patient. Then some hypothesis of etiopathogenetic mechanism of this rhythm disturbance in patients affected with Friedreich's ataxia are proposed.", "contents": "[A rare case of Friedreich's atoxia with cardiac involvement: the torsade de pointe (author's transl)]. The Authors describe a case of Friedreich's ataxia complicated by an unusual arrhythmia, torsade de pointes, the only manifestation of cardiac involvment in this patient. Then some hypothesis of etiopathogenetic mechanism of this rhythm disturbance in patients affected with Friedreich's ataxia are proposed."}
{"id": "PMID:488599", "title": "[Changes in precordial movements in non-ischaemic heart disease patients after i.v. administration of isoproterenol and dopamine. Comparison between normal and cardiopathic subjects (author's transl)].", "content": "18 patients with non-ischemic heart disease have been studied with reference to the kinetocardiographic changes before and after i.v. administration of dopamine (DOP) (Revivan) or Isoproterenol (ISP) (Aleudrin). For this purpose a simultaneous registration of ECG (lead D2), Kinetocardiogram (KCG) in K25 and K45 was performed. Volume curves were considered as a normal features of KCG, curves showing systolic outward movements during the ejection phase were classified as pathologic. The reduction or disappearance of the systolic movements during the drug administration, were considered as kinetic improvement, the increase of their site was judged as kinetic worsening. During the infusion, 17 KCGs maintained a normal morphological pattern; out of 19 pathological KCGs, 4 did not show any change, and 15, 5 reached a complete normalization. It is pointed out the overlap of the precordial kinetics in the ventricular hypertrophies and in the normal subjects (kinetic improvement), and the possibility to differentiate a systolic outward movement due to synergic contraction of hypertrophied ventricle from a systolic outward movement to be reffered to a diskynetic contraction in the myocardial ischemia (kinetic worsening).", "contents": "[Changes in precordial movements in non-ischaemic heart disease patients after i.v. administration of isoproterenol and dopamine. Comparison between normal and cardiopathic subjects (author's transl)]. 18 patients with non-ischemic heart disease have been studied with reference to the kinetocardiographic changes before and after i.v. administration of dopamine (DOP) (Revivan) or Isoproterenol (ISP) (Aleudrin). For this purpose a simultaneous registration of ECG (lead D2), Kinetocardiogram (KCG) in K25 and K45 was performed. Volume curves were considered as a normal features of KCG, curves showing systolic outward movements during the ejection phase were classified as pathologic. The reduction or disappearance of the systolic movements during the drug administration, were considered as kinetic improvement, the increase of their site was judged as kinetic worsening. During the infusion, 17 KCGs maintained a normal morphological pattern; out of 19 pathological KCGs, 4 did not show any change, and 15, 5 reached a complete normalization. It is pointed out the overlap of the precordial kinetics in the ventricular hypertrophies and in the normal subjects (kinetic improvement), and the possibility to differentiate a systolic outward movement due to synergic contraction of hypertrophied ventricle from a systolic outward movement to be reffered to a diskynetic contraction in the myocardial ischemia (kinetic worsening)."}
{"id": "PMID:488606", "title": "Radiologic assessment of resectability and prognosis in esophageal carcinoma.", "content": "Utilizing double-contrast technique it is possible to detect the earliest malignant lesions of the esophagus even when the carcinoma is confined to the mucosa. In patients with carcinoma, it is also important to attempt to grade and classify the lesion in order to predict its resectability and prognosis. The resectability of a lesion is determined by the depth of invasion and by the presence or absence of lymph node metastases. The significant factors are the length of the lesion, its gross morphology, the character of its margins, and the depth of ulceration. Unfortunately, the depth of invasion is not necessarily directly related to the prognosis. In some carcinomas confined to the submucosa, lymph node metastases are already present, and these patients have a poor prognosis. The presence of lymph node metastases in early carcinoma can be suggested by the gross morphology of the lesion, its length, irregularity of the surface, and the presence of a complex lesion. Careful analysis of the radiographic features of the carcinoma can be valuable for the assessment of resectability and prognosis.", "contents": "Radiologic assessment of resectability and prognosis in esophageal carcinoma. Utilizing double-contrast technique it is possible to detect the earliest malignant lesions of the esophagus even when the carcinoma is confined to the mucosa. In patients with carcinoma, it is also important to attempt to grade and classify the lesion in order to predict its resectability and prognosis. The resectability of a lesion is determined by the depth of invasion and by the presence or absence of lymph node metastases. The significant factors are the length of the lesion, its gross morphology, the character of its margins, and the depth of ulceration. Unfortunately, the depth of invasion is not necessarily directly related to the prognosis. In some carcinomas confined to the submucosa, lymph node metastases are already present, and these patients have a poor prognosis. The presence of lymph node metastases in early carcinoma can be suggested by the gross morphology of the lesion, its length, irregularity of the surface, and the presence of a complex lesion. Careful analysis of the radiographic features of the carcinoma can be valuable for the assessment of resectability and prognosis."}
{"id": "PMID:488607", "title": "Delayed esophageal perforation after pneumatic dilatation for the treatment of achalasia.", "content": "Two cases of delayed esophageal perforation following a pneumatic dilatation for the treatment of achalasia are presented. Esophagrams obtained immediately after pneumatic dilatation failed to reveal a perforation. Increasing symptoms prompted repeat contrast studies, at which time an esophageal perforation was demonstrated. The occurrence of delayed esophageal perforation after pneumatic intrumentation is emphasized. An ischemic etiology for this delayed perforation is postulated.", "contents": "Delayed esophageal perforation after pneumatic dilatation for the treatment of achalasia. Two cases of delayed esophageal perforation following a pneumatic dilatation for the treatment of achalasia are presented. Esophagrams obtained immediately after pneumatic dilatation failed to reveal a perforation. Increasing symptoms prompted repeat contrast studies, at which time an esophageal perforation was demonstrated. The occurrence of delayed esophageal perforation after pneumatic intrumentation is emphasized. An ischemic etiology for this delayed perforation is postulated."}
{"id": "PMID:488608", "title": "Spontaneous closure of esophageal tear in Boerhaave's syndrome.", "content": "An unusual case of spontaneous rupture of the esophagus is reported in which the patient survived without surgical closure or drainage procedure. The site of perforation and subsequent closure are documented radiologically.", "contents": "Spontaneous closure of esophageal tear in Boerhaave's syndrome. An unusual case of spontaneous rupture of the esophagus is reported in which the patient survived without surgical closure or drainage procedure. The site of perforation and subsequent closure are documented radiologically."}
{"id": "PMID:488609", "title": "Unusual leiomyoblastoma of the stomach.", "content": "Only about 250 cases of benign gastric leiomyoblastoma of the stomach have been reported. Most occur in the smooth muscle of the antrum, and may be intramural, intraluminal, or exogastric in position. A pedunculated gastric leiomyoblastoma occurs less frequently, and those containing radiographically identifiable calcifications are extremely rare.", "contents": "Unusual leiomyoblastoma of the stomach. Only about 250 cases of benign gastric leiomyoblastoma of the stomach have been reported. Most occur in the smooth muscle of the antrum, and may be intramural, intraluminal, or exogastric in position. A pedunculated gastric leiomyoblastoma occurs less frequently, and those containing radiographically identifiable calcifications are extremely rare."}
{"id": "PMID:488610", "title": "Angiographic evaluation of the abnormal endoscopic pancreatogram.", "content": "Out of 1,269 pancreatograms, 122 were abnormal. Angiography was performed in these patients. Fifty-five were found to have pancreatic carcinoma. In the remaining 67 patients a false positive angiographic diagnosis of either chronic pancreatitis or pancreatic cancer was made in 11%. In one patient a hemangioma was diagnosed as a pancreatic cyst. The remaining 58 patients all had normal pancreatic angiograms in spite of gross ductal abnormality on endoscopic retrograde cholangiopancreatography (ERCP). All these patients were followed for an average of 19 months and showed no clinical evidence of pancreatic disease. It is suggested that angiography should be considered a complementary examination to ERCP and is particularly useful to exclude carcinoma when the pancreatogram is abnormal.", "contents": "Angiographic evaluation of the abnormal endoscopic pancreatogram. Out of 1,269 pancreatograms, 122 were abnormal. Angiography was performed in these patients. Fifty-five were found to have pancreatic carcinoma. In the remaining 67 patients a false positive angiographic diagnosis of either chronic pancreatitis or pancreatic cancer was made in 11%. In one patient a hemangioma was diagnosed as a pancreatic cyst. The remaining 58 patients all had normal pancreatic angiograms in spite of gross ductal abnormality on endoscopic retrograde cholangiopancreatography (ERCP). All these patients were followed for an average of 19 months and showed no clinical evidence of pancreatic disease. It is suggested that angiography should be considered a complementary examination to ERCP and is particularly useful to exclude carcinoma when the pancreatogram is abnormal."}
{"id": "PMID:488611", "title": "Ultrasonographic demonstration of the pancreatic duct.", "content": "During a 4-year period, 375 patients with clinically suggestive pancreatic disease were examined with gray scale ultrasound. In 14 patients, the pancreatic duct itself was demonstrated. All of these had clinical or laboratory evidence for, and radiologically or surgically proven, pancreatic duct obstruction. Etiology of the obstruction was either tumor, chronic pancreatitis, pancreatic abscess, pseudocyst, or stricture of the duct at the papilla of Vater. Two patients had Crohn's disease of the duodenum.", "contents": "Ultrasonographic demonstration of the pancreatic duct. During a 4-year period, 375 patients with clinically suggestive pancreatic disease were examined with gray scale ultrasound. In 14 patients, the pancreatic duct itself was demonstrated. All of these had clinical or laboratory evidence for, and radiologically or surgically proven, pancreatic duct obstruction. Etiology of the obstruction was either tumor, chronic pancreatitis, pancreatic abscess, pseudocyst, or stricture of the duct at the papilla of Vater. Two patients had Crohn's disease of the duodenum."}
{"id": "PMID:488612", "title": "Ultrasonic characteristics of pancreatic tumors.", "content": "The ultrasonic features of 40 pancreatic tumors were analyzed. The tumors were categorized by texture, attenuation, shape, size, and location. Secondary effects of pancreatic duct visualization, biliary stasis, splenic vein involvement, metastases, and ascites were reviewed. Thirty of thrity-one adenocarcinomas had a similar ultrasonic texture consisting of a background of faint, low-level echoes with varying amounts of superimposed coarse echoes. Tumors are detectable prior to enlargement or distortion of the pancreas. Secondary features were present in 94% of the adenocarcinomas. Significant information regarding the possibility and complexity of resection can be provided.", "contents": "Ultrasonic characteristics of pancreatic tumors. The ultrasonic features of 40 pancreatic tumors were analyzed. The tumors were categorized by texture, attenuation, shape, size, and location. Secondary effects of pancreatic duct visualization, biliary stasis, splenic vein involvement, metastases, and ascites were reviewed. Thirty of thrity-one adenocarcinomas had a similar ultrasonic texture consisting of a background of faint, low-level echoes with varying amounts of superimposed coarse echoes. Tumors are detectable prior to enlargement or distortion of the pancreas. Secondary features were present in 94% of the adenocarcinomas. Significant information regarding the possibility and complexity of resection can be provided."}
{"id": "PMID:488613", "title": "Primary common bile duct carcinoid.", "content": "Endoscopic retrograde cholangiography was performed in a 32-year-old man with recurrent episodes of painless jaundice. There was demonstration of a large intraluminal mass causing partial obstruction of the common bile duct. The lesion proved to be a primary carcinoid tumor of the bile duct.", "contents": "Primary common bile duct carcinoid. Endoscopic retrograde cholangiography was performed in a 32-year-old man with recurrent episodes of painless jaundice. There was demonstration of a large intraluminal mass causing partial obstruction of the common bile duct. The lesion proved to be a primary carcinoid tumor of the bile duct."}
{"id": "PMID:488614", "title": "Spontaneous disappearance of gallstones.", "content": "A case of spontaneous disappearance of gallstones in a 55-year-old woman is presented. Examination of biliary lipids after the stones had disappeared revealed supersaturated bile with cholesterol. This suggests that the stones migrated from the gallbladder to the small intestine via the biliary ductal system.", "contents": "Spontaneous disappearance of gallstones. A case of spontaneous disappearance of gallstones in a 55-year-old woman is presented. Examination of biliary lipids after the stones had disappeared revealed supersaturated bile with cholesterol. This suggests that the stones migrated from the gallbladder to the small intestine via the biliary ductal system."}
{"id": "PMID:488615", "title": "Recurrent appendicitis: reexamination of a controversial disease.", "content": "The existence of recurrent appendicitis as a true disease entity is still disputed. The controversy surrounding recurrent appendicitis is discussed. This disease is thought to represent repeated inflammation of a nonperforated appendix. Case material supporting this concept is presented. The barium enema can provide evidence of the diseased appendix.", "contents": "Recurrent appendicitis: reexamination of a controversial disease. The existence of recurrent appendicitis as a true disease entity is still disputed. The controversy surrounding recurrent appendicitis is discussed. This disease is thought to represent repeated inflammation of a nonperforated appendix. Case material supporting this concept is presented. The barium enema can provide evidence of the diseased appendix."}
{"id": "PMID:488616", "title": "Simultaneous double obstruction of the proximal colon: a case report.", "content": "A rare case of simultaneous obstructions of the proximal colon secondary to an obstructing carcinoma of the transverse colon and an ascending colon volvulus at the hepatic flexure is presented. Their probable interrelationship and resulting radiographic features are discussed.", "contents": "Simultaneous double obstruction of the proximal colon: a case report. A rare case of simultaneous obstructions of the proximal colon secondary to an obstructing carcinoma of the transverse colon and an ascending colon volvulus at the hepatic flexure is presented. Their probable interrelationship and resulting radiographic features are discussed."}
{"id": "PMID:488617", "title": "Pneumoperitoneum occurring during double-contrast enema.", "content": "A case of pneumoperitoneum occurring during double-contrast barium enema is described. Cases reported to date indicate that colonic rupture during a double-contrast enema is likely to result in exit of air rather than barium from the colon. The diagnostic implicatons of this observation are considered. The potentially high pressure generated by bulb-type inflators is discussed, and caustion is advised against overdistending the colon during double-contrast enemas.", "contents": "Pneumoperitoneum occurring during double-contrast enema. A case of pneumoperitoneum occurring during double-contrast barium enema is described. Cases reported to date indicate that colonic rupture during a double-contrast enema is likely to result in exit of air rather than barium from the colon. The diagnostic implicatons of this observation are considered. The potentially high pressure generated by bulb-type inflators is discussed, and caustion is advised against overdistending the colon during double-contrast enemas."}
{"id": "PMID:488618", "title": "Retroperitoneal lipomatosis--report of two cases with an unusual distribution.", "content": "Two cases of retroperitoneal lipomatosis with an unusual distribution are reported. In the first case the fat was confined to the perirectal soft tissues and the sigmoid mesentery, whereas in the second case it was peripancreatic and extended into the small bowel mesentery. The lipomatous nature of these retroperitoneal masses was suggested by the lucency of the fat on the plain abdominal roentgenograms. These cases were studied prior to the availability of computed tomography, so that the diagnoses were confirmed by surgical exploration, which may have been avoided by this currently available noninvasive technique.", "contents": "Retroperitoneal lipomatosis--report of two cases with an unusual distribution. Two cases of retroperitoneal lipomatosis with an unusual distribution are reported. In the first case the fat was confined to the perirectal soft tissues and the sigmoid mesentery, whereas in the second case it was peripancreatic and extended into the small bowel mesentery. The lipomatous nature of these retroperitoneal masses was suggested by the lucency of the fat on the plain abdominal roentgenograms. These cases were studied prior to the availability of computed tomography, so that the diagnoses were confirmed by surgical exploration, which may have been avoided by this currently available noninvasive technique."}
{"id": "PMID:488619", "title": "Infusion tomography and ultrasonography of the gallbladder in the diagnosis of acute cholecystitis.", "content": "Sixty-three patients with the clinical suspicion of acute cholecystitis were examined with infusion tomography of the gallbladder. Ultrasonography was performed in 51 of these cases. The technique and diagnostic principles of both methods when used in combination is stressed. Thus in a case of gangrenous cholecystitis when opacification of the gallbladder wall may not appear at infusion tomography, ultrasonography may demonstrate signs of gallbladder disease. Infusion tomography, on the other hand, may be of great value if ultrasonography is not informative.", "contents": "Infusion tomography and ultrasonography of the gallbladder in the diagnosis of acute cholecystitis. Sixty-three patients with the clinical suspicion of acute cholecystitis were examined with infusion tomography of the gallbladder. Ultrasonography was performed in 51 of these cases. The technique and diagnostic principles of both methods when used in combination is stressed. Thus in a case of gangrenous cholecystitis when opacification of the gallbladder wall may not appear at infusion tomography, ultrasonography may demonstrate signs of gallbladder disease. Infusion tomography, on the other hand, may be of great value if ultrasonography is not informative."}
{"id": "PMID:488620", "title": "Enterocylsis: the small bowel enema. How to succeed and how to fail.", "content": "Enteroclysis, the infusion of contrast medium directly into the small bowel, is a precise, rapid method for thorough small bowel examination. This technique demonstrates far more pathology than any other method when it is properly executed. However, the examination is sure to fail with poor techniques, thereby discrediting a superb method. Experience has shown that failures are usually due to inadequate technique or poor judgment or both during the examination. This article covers the proper and improper techniques of the examination and gives examples of each. The most common problems that arise during the examination are discussed in the order in which they usually develop.", "contents": "Enterocylsis: the small bowel enema. How to succeed and how to fail. Enteroclysis, the infusion of contrast medium directly into the small bowel, is a precise, rapid method for thorough small bowel examination. This technique demonstrates far more pathology than any other method when it is properly executed. However, the examination is sure to fail with poor techniques, thereby discrediting a superb method. Experience has shown that failures are usually due to inadequate technique or poor judgment or both during the examination. This article covers the proper and improper techniques of the examination and gives examples of each. The most common problems that arise during the examination are discussed in the order in which they usually develop."}
{"id": "PMID:488621", "title": "A clinical study of tuberculous colitis.", "content": "During the last 8 years, the diagnosis of tuberculous colitis has been made radiologically in 22 cases. Twelve were confirmed by histological or bacteriological evidences. The X-ray findings are classified into three types as follows: Type A, showing an extensive scarred area in the ileocecum and ascending colon. Type B, showing annular stricture in the ascending colon and dilatation of its oral side. Type C, showing pouchformation (pseudo-diverticulum) and deformity in the cecum. We emphasize that histological examination and culture of the biopsy specimens by endoscopy were useful for the diagnosis of tuberculous colitis. Furthermore, radiologic changes of the colon by administration of antituberculous drugs were observed.", "contents": "A clinical study of tuberculous colitis. During the last 8 years, the diagnosis of tuberculous colitis has been made radiologically in 22 cases. Twelve were confirmed by histological or bacteriological evidences. The X-ray findings are classified into three types as follows: Type A, showing an extensive scarred area in the ileocecum and ascending colon. Type B, showing annular stricture in the ascending colon and dilatation of its oral side. Type C, showing pouchformation (pseudo-diverticulum) and deformity in the cecum. We emphasize that histological examination and culture of the biopsy specimens by endoscopy were useful for the diagnosis of tuberculous colitis. Furthermore, radiologic changes of the colon by administration of antituberculous drugs were observed."}
{"id": "PMID:488622", "title": "Endoscopic electromyography of the sphincter of Oddi.", "content": "Endoscopic electromyography of the human sphincter of ODDI has provided useful information for the patho-physiological studies on the choledocho-duodenal junction. The pattern and rhythm of the electromyogram observed on the spincter of ODDI were different from those of the duodenum recorded simultaneously in basal tracings of our initial four cases. In the half of other ten cases with consecutive tracings, the different pattern and rhythm of electromyograms between the sphincter of ODDI and the duodenum were also confirmed. It was possible, from our results, to speculate that the functional independence of the sphincter of ODDI from the duodenal wall muscles was in existence.", "contents": "Endoscopic electromyography of the sphincter of Oddi. Endoscopic electromyography of the human sphincter of ODDI has provided useful information for the patho-physiological studies on the choledocho-duodenal junction. The pattern and rhythm of the electromyogram observed on the spincter of ODDI were different from those of the duodenum recorded simultaneously in basal tracings of our initial four cases. In the half of other ten cases with consecutive tracings, the different pattern and rhythm of electromyograms between the sphincter of ODDI and the duodenum were also confirmed. It was possible, from our results, to speculate that the functional independence of the sphincter of ODDI from the duodenal wall muscles was in existence."}
{"id": "PMID:488623", "title": "Changes of pancreatic exocrine function after major resection of the pancreas in dogs.", "content": "Changes of an exocrine function after major resection of the canine pancreas were observed by pancreozymin-secretin test and the results were compared with an endocrine function examined by glucose tolerance test. The exocrine function of the remnant pancreas revealed characteristic changes according to the resection rate of the pancreas: 1) After removal of 50 to 70 per cent of the entire pancreas, diabetes did not develop and the exocrine function of the remnant pancreas was well maintained. 2) After 70 to 90 per cent pancreatectomy, diabetes developed six weeks or more later and the exocrine function of the remnant pancreas per body weight decreased slightly. The degree of disturbance of the exocrine function in the diabetic dogs was greater than that in the non-diabetic group, but the exocrine function per the remnant pancreas weight increased and it was greater than normal level even in the diabetic dogs. The hypersecretory state of the exocrine pancreas after surgery was verified. 3) After 90 per cent or greater resection of the pancreas, diabetes occurred immediately and disturbance of the exocrine function was remarkable. Hypersecretory state did not appear. 4) Both the endocrine and exocrine functions after major pancreatic resection were found to be related to the resection rate, but there were significant differences in the reserve capacity of the endocrine and exocrine pancreas.", "contents": "Changes of pancreatic exocrine function after major resection of the pancreas in dogs. Changes of an exocrine function after major resection of the canine pancreas were observed by pancreozymin-secretin test and the results were compared with an endocrine function examined by glucose tolerance test. The exocrine function of the remnant pancreas revealed characteristic changes according to the resection rate of the pancreas: 1) After removal of 50 to 70 per cent of the entire pancreas, diabetes did not develop and the exocrine function of the remnant pancreas was well maintained. 2) After 70 to 90 per cent pancreatectomy, diabetes developed six weeks or more later and the exocrine function of the remnant pancreas per body weight decreased slightly. The degree of disturbance of the exocrine function in the diabetic dogs was greater than that in the non-diabetic group, but the exocrine function per the remnant pancreas weight increased and it was greater than normal level even in the diabetic dogs. The hypersecretory state of the exocrine pancreas after surgery was verified. 3) After 90 per cent or greater resection of the pancreas, diabetes occurred immediately and disturbance of the exocrine function was remarkable. Hypersecretory state did not appear. 4) Both the endocrine and exocrine functions after major pancreatic resection were found to be related to the resection rate, but there were significant differences in the reserve capacity of the endocrine and exocrine pancreas."}
{"id": "PMID:488624", "title": "Role of plasma histamine in liver injury--clinical and experimental investigations.", "content": "Plasma histamine level (PHL) was evaluated by a modified fluorometric assay (Suzuki) in the patients with various forms of liver disease as well as rabbits with liver injury. And the data obtained were compared with liver function tests in assessing the stage and prognosis of hepatic dysfunction. In acute hepatitis, if its prognosis was \"good\", as was also shown in the animal group with single dose administration of CCl4, the level of plasma histamine attained a peak before that of serum transaminases, and returned to normal prior to that of the latter. In persistent and chronic hepatitis, although correlation between PHL and other liver function tests was poor and variable, PHL remained high. And the estimation of PHL during the course of this state showed that it was elevated prior to that of serum transaminases, in dicating high level of plasma histamine in this state, even in apparent \"steady state\", worsening of the disease. In liver cirrhosis PHL correlated with the degree of serum transaminases as well as serum gammaglobulin. In \"poor prognosis\" group (patients with hepatic coma and rabbits treated with consecutive administration of CCl4) PHL increased extremely high, which was contrasted with the lowered levels of transaminases. These results strikingly suggest that histamine is involved in liver injury and estimation of PHL in the course of hepatic disorder is useful for a prediction of prognosis.", "contents": "Role of plasma histamine in liver injury--clinical and experimental investigations. Plasma histamine level (PHL) was evaluated by a modified fluorometric assay (Suzuki) in the patients with various forms of liver disease as well as rabbits with liver injury. And the data obtained were compared with liver function tests in assessing the stage and prognosis of hepatic dysfunction. In acute hepatitis, if its prognosis was \"good\", as was also shown in the animal group with single dose administration of CCl4, the level of plasma histamine attained a peak before that of serum transaminases, and returned to normal prior to that of the latter. In persistent and chronic hepatitis, although correlation between PHL and other liver function tests was poor and variable, PHL remained high. And the estimation of PHL during the course of this state showed that it was elevated prior to that of serum transaminases, in dicating high level of plasma histamine in this state, even in apparent \"steady state\", worsening of the disease. In liver cirrhosis PHL correlated with the degree of serum transaminases as well as serum gammaglobulin. In \"poor prognosis\" group (patients with hepatic coma and rabbits treated with consecutive administration of CCl4) PHL increased extremely high, which was contrasted with the lowered levels of transaminases. These results strikingly suggest that histamine is involved in liver injury and estimation of PHL in the course of hepatic disorder is useful for a prediction of prognosis."}
{"id": "PMID:488625", "title": "Serum glycoproteins in the liver diseases. VIII. Desialylated glycoproteins in the liver cirrhosis.", "content": "Serum desialylated glycoprotein livel of cirrhotic patients was determined and a diagnostically significant elevation of these proteins was observed. The level of these patients was usually 2--10 times of that seen in normal subjects and the elevation was signifi-ant (p less than 0.001) when compared to the level in patients with chronic aggressive hepatitis, severe (2B). Serial determinations of these proteins in the cirrhotic patients showed no correlation between them and SGPT as a whole but in several cases in which SGPT fluctuated the former associated with the latter. In patients with decompensated cirrhotic liver these proteins returned nearly to the level of compensated patients when it was improved. The level of these proteins in cirrhotic patients correlated, not always, with serum albumin (r = -0.46, p less than 0.02) and indocyanine green clearance rate (r = -0.73, p less than 0.05), but not with SGPT as well as the other liver function tests.", "contents": "Serum glycoproteins in the liver diseases. VIII. Desialylated glycoproteins in the liver cirrhosis. Serum desialylated glycoprotein livel of cirrhotic patients was determined and a diagnostically significant elevation of these proteins was observed. The level of these patients was usually 2--10 times of that seen in normal subjects and the elevation was signifi-ant (p less than 0.001) when compared to the level in patients with chronic aggressive hepatitis, severe (2B). Serial determinations of these proteins in the cirrhotic patients showed no correlation between them and SGPT as a whole but in several cases in which SGPT fluctuated the former associated with the latter. In patients with decompensated cirrhotic liver these proteins returned nearly to the level of compensated patients when it was improved. The level of these proteins in cirrhotic patients correlated, not always, with serum albumin (r = -0.46, p less than 0.02) and indocyanine green clearance rate (r = -0.73, p less than 0.05), but not with SGPT as well as the other liver function tests."}
{"id": "PMID:488626", "title": "Crohn's disease in Japan: The Japanese Research Committee for Crohn's Disease.", "content": "Sixty four cases of Crohn's disease were diagnosed. From our resluts reported here, the following conclusions were confirmed: 1) Crohn's disease as reported in Western countries certainly occurs among Japanese, too. 2) The most characteristic feature of Crohn's disease in Japanese patients was a long longitudinal ulcer along the mesenteric attachment of the small intestine, in addition to a cobblestone appearance. 3) In establishing the diagnosis of Crohn's disease, it was most important to distinguish Crohn's disease from intestinal tuberculosis.", "contents": "Crohn's disease in Japan: The Japanese Research Committee for Crohn's Disease. Sixty four cases of Crohn's disease were diagnosed. From our resluts reported here, the following conclusions were confirmed: 1) Crohn's disease as reported in Western countries certainly occurs among Japanese, too. 2) The most characteristic feature of Crohn's disease in Japanese patients was a long longitudinal ulcer along the mesenteric attachment of the small intestine, in addition to a cobblestone appearance. 3) In establishing the diagnosis of Crohn's disease, it was most important to distinguish Crohn's disease from intestinal tuberculosis."}
{"id": "PMID:488627", "title": "Congenital multiple cystic dilatation of the intrahepatic biliary ducts (two cases with simple type of Caroli's disease).", "content": "Two cases of Caroli's diseases (simple type) precisely diagnosed prior to the operation by the direct cholangiography, endoscopic retrograde cholangiography, and percutaneous transhepatic cholangiography, were reported. They both had a long cystic duct, and association of cystic lesions of bilateral kidneys was revealed in one of them. Excessive biliary output had been observed postoperatively in both of them. A cholecystectomy and choledochodrainage successfully gave rise to favourable results in both cases reported.", "contents": "Congenital multiple cystic dilatation of the intrahepatic biliary ducts (two cases with simple type of Caroli's disease). Two cases of Caroli's diseases (simple type) precisely diagnosed prior to the operation by the direct cholangiography, endoscopic retrograde cholangiography, and percutaneous transhepatic cholangiography, were reported. They both had a long cystic duct, and association of cystic lesions of bilateral kidneys was revealed in one of them. Excessive biliary output had been observed postoperatively in both of them. A cholecystectomy and choledochodrainage successfully gave rise to favourable results in both cases reported."}
{"id": "PMID:488628", "title": "Reduction of twenty-four-hour gastric acidity with combination drug therapy in patients with duodenal ulcer.", "content": "Four \"extra-effort\" drug regimens were tested to determine which most nearly eliminated 24-hr gastric acidity in 8 patients with duodenal ulcer. The regimens included two 300 mg cimetidine tablets with meals and at bedtime; one 300 mg cimetidine tablet plus an anticholinergic drug with meals and at bedtime; 300 mg cimetidine with meals and at bedtime plus liquid antacid 1 and 3 hr after meals and at bedtime; and 300 mg cimetidine, an anticholinergic drug, and antacid, taken simultaneously as each meal was finished and at bedtime. No regimen completely eliminated gastric acidity. However, compared to standard cimetidine therapy (300 mg four times daily) which led to a median 24-hr pH of 2.6, each \"extra-effort\" regimen except cimetidine plus an anticholinergic was significantly better in reducing gastric acidity. During the daytime hours, cimetidine with meals plus antacid 1 and 3 hr after meals was most effective (median pH 5.0). However, the more convenient regimen of cimetidine, an anticholinergic drug, and antacid was almost as effective (median pH 4.3). None of the \"extra-effort\" regimens was significantly more effective than standard cimetidine therapy during the hours of sleep.", "contents": "Reduction of twenty-four-hour gastric acidity with combination drug therapy in patients with duodenal ulcer. Four \"extra-effort\" drug regimens were tested to determine which most nearly eliminated 24-hr gastric acidity in 8 patients with duodenal ulcer. The regimens included two 300 mg cimetidine tablets with meals and at bedtime; one 300 mg cimetidine tablet plus an anticholinergic drug with meals and at bedtime; 300 mg cimetidine with meals and at bedtime plus liquid antacid 1 and 3 hr after meals and at bedtime; and 300 mg cimetidine, an anticholinergic drug, and antacid, taken simultaneously as each meal was finished and at bedtime. No regimen completely eliminated gastric acidity. However, compared to standard cimetidine therapy (300 mg four times daily) which led to a median 24-hr pH of 2.6, each \"extra-effort\" regimen except cimetidine plus an anticholinergic was significantly better in reducing gastric acidity. During the daytime hours, cimetidine with meals plus antacid 1 and 3 hr after meals was most effective (median pH 5.0). However, the more convenient regimen of cimetidine, an anticholinergic drug, and antacid was almost as effective (median pH 4.3). None of the \"extra-effort\" regimens was significantly more effective than standard cimetidine therapy during the hours of sleep."}
{"id": "PMID:488629", "title": "Electrical and mechanical activity in the ex vivo perfused total canine colon.", "content": "Electrical and mechanical activity were studied in the ex vivo total canine colon, supported in a perfusion chamber by a supporting dog. Regular electrical activity could be recognized visually in 50--70% of the records. The frequency of this activity was in the range of 7--10/min. Fourier analysis also revealed dominant frequencies in this range. Two types of mechanical activity were observed. The first consisted of small amplitude repetitive contractions, which appeared both during quiescent periods and during vigorous contractions. The frequency of this rippling, when periodic, usually approximated that of the electrical activity. The second type consisted of large amplitude prolonged (30--135 sec) contractions. Without stimulation, the large amplitude contractions were asynchronous and not propagated. Following neostigmine, however, the large amplitude contractions were always propagated aborally and were usually associated with evacuation of the colon. The rippling contractions had a frequency similar to the regular electrical activity and are probably under their control. The large amplitude, prolonged duration contractions are unlikely, however, to be solely under myogenic control.", "contents": "Electrical and mechanical activity in the ex vivo perfused total canine colon. Electrical and mechanical activity were studied in the ex vivo total canine colon, supported in a perfusion chamber by a supporting dog. Regular electrical activity could be recognized visually in 50--70% of the records. The frequency of this activity was in the range of 7--10/min. Fourier analysis also revealed dominant frequencies in this range. Two types of mechanical activity were observed. The first consisted of small amplitude repetitive contractions, which appeared both during quiescent periods and during vigorous contractions. The frequency of this rippling, when periodic, usually approximated that of the electrical activity. The second type consisted of large amplitude prolonged (30--135 sec) contractions. Without stimulation, the large amplitude contractions were asynchronous and not propagated. Following neostigmine, however, the large amplitude contractions were always propagated aborally and were usually associated with evacuation of the colon. The rippling contractions had a frequency similar to the regular electrical activity and are probably under their control. The large amplitude, prolonged duration contractions are unlikely, however, to be solely under myogenic control."}
{"id": "PMID:488630", "title": "Enterogastric reflux in normal subjects and patients with Bilroth II gastroenterostomy. Measurement of enterogastric reflux.", "content": "Initially, scintigraphy was established as a valid method for detecting and quantitating enterogastric reflux. A new, tubeless technique for the measurement of enterogastric reflux was developed. 99mTc bound to [(2,6 dimethylphenylcarbamoylmethyl) iminodiacetic acid] (5 mCi) was administered intravenously to visualize the liver and biliary tract. One hour later, a standard liquid meal labeled with 111In bound to diethylene-triamine penta-acetic acid (250 microCi) was given. The 99mTc and 111In activities were recorded simultaneously for 1-min periods at 15-min intervals for 2 hr over liver, gallbladder, and gastric areas of interest. Enterogastric reflux indices were determined. Ten normal subjects and 13 patients with vagotomy, hemigastrectomy, and Bilroth II gastrojejunostomy were evaluated. The enterogastric reflux index in asymptomatic postsurgical patients was increased significantly to 24.6 +/- 4.7 compared with 8.2 +/- 6.0 (P less than 0.01) in normal subjects. In postsurgical patients with the syndrome of alkaline gastritis, the enterogastric reflux index was increased significantly to 86.3 +/- 7.1 (P less than 0.01) compared with asymptomatic postsurgical patients.", "contents": "Enterogastric reflux in normal subjects and patients with Bilroth II gastroenterostomy. Measurement of enterogastric reflux. Initially, scintigraphy was established as a valid method for detecting and quantitating enterogastric reflux. A new, tubeless technique for the measurement of enterogastric reflux was developed. 99mTc bound to [(2,6 dimethylphenylcarbamoylmethyl) iminodiacetic acid] (5 mCi) was administered intravenously to visualize the liver and biliary tract. One hour later, a standard liquid meal labeled with 111In bound to diethylene-triamine penta-acetic acid (250 microCi) was given. The 99mTc and 111In activities were recorded simultaneously for 1-min periods at 15-min intervals for 2 hr over liver, gallbladder, and gastric areas of interest. Enterogastric reflux indices were determined. Ten normal subjects and 13 patients with vagotomy, hemigastrectomy, and Bilroth II gastrojejunostomy were evaluated. The enterogastric reflux index in asymptomatic postsurgical patients was increased significantly to 24.6 +/- 4.7 compared with 8.2 +/- 6.0 (P less than 0.01) in normal subjects. In postsurgical patients with the syndrome of alkaline gastritis, the enterogastric reflux index was increased significantly to 86.3 +/- 7.1 (P less than 0.01) compared with asymptomatic postsurgical patients."}
{"id": "PMID:488631", "title": "Intestinal uptake of macromolecules. VI. Uptake of protein antigen in vivo in normal rats and in rats infected with Nippostrongylus brasiliensis or subjected to mild systemic anaphylaxis.", "content": "Adult Sprague-Dawley rats weighing approximately g were fed bovine serum albumin and sodium bicarbonate by gavage. Serum was obtained at intervals after feeding and tested for immunoreactive bovine serum albumin by radioimmunoassay. Nanogram amounts of immunoreactive bovine serum albumin were detected in serum; peak values were obtained after 4 and 6 hr. The influence of intestinal inflammation on protein uptake was examined in two model systems. Infection of rats with Nippostrongylus brasiliensis was accompanied by partial villous atrophy in the intestinal segments harboring adult worms and mild systemic anaphylaxis in the rat was accompanied by increased intestinal vascular and mucosal permeability. Enhanced uptake of BSA was observed before and shortly after self-cure of infection and during mild systemic anaphylaxis. The molecular size of immunoreactive bovine serum albumin approximated that of the administered bovine serum albumin; no small fragments of bovine serum albumin bearing antigenic determinants were detected.", "contents": "Intestinal uptake of macromolecules. VI. Uptake of protein antigen in vivo in normal rats and in rats infected with Nippostrongylus brasiliensis or subjected to mild systemic anaphylaxis. Adult Sprague-Dawley rats weighing approximately g were fed bovine serum albumin and sodium bicarbonate by gavage. Serum was obtained at intervals after feeding and tested for immunoreactive bovine serum albumin by radioimmunoassay. Nanogram amounts of immunoreactive bovine serum albumin were detected in serum; peak values were obtained after 4 and 6 hr. The influence of intestinal inflammation on protein uptake was examined in two model systems. Infection of rats with Nippostrongylus brasiliensis was accompanied by partial villous atrophy in the intestinal segments harboring adult worms and mild systemic anaphylaxis in the rat was accompanied by increased intestinal vascular and mucosal permeability. Enhanced uptake of BSA was observed before and shortly after self-cure of infection and during mild systemic anaphylaxis. The molecular size of immunoreactive bovine serum albumin approximated that of the administered bovine serum albumin; no small fragments of bovine serum albumin bearing antigenic determinants were detected."}
{"id": "PMID:488633", "title": "Transformation of chenodeoxycholic acid and ursodeoxycholic acid by human intestinal bacteria.", "content": "Feces from normal subjects and patients with cerebrotendinous xanthomatosis were incubated anaerobically with labeled chenodeoxycholic acid and ursodeoxycholine acid for known periods, and the bile acids formed were analyzed by TLC and scintillation counting. In the normal subjects, 80% of the chenodeoxycholic acid and 41% of the ursodeoxycholic acid were 7-dehydroxylated to lithocholic acid during 2 hr of incubation. In contrast, the fecal flora of the CTX patients transformed only 5% of chenodeoxycholic acid and less than 1% of ursodeoxycholic acid to lithocholic acid during the same time period. In several subjects (normals and CTX), the intestinal flora converted chenodeoxycholic acid to ursodeoxycholic acid without the accumulation of the hypothetical intermediate 7-ketolithocholic acid (3 alpha-hydroxy-7-keto-5 beta-cholanoic acid). These results indicate that the fecal bacterial flora is capable of 7-dehydroxylating chenodeoxycholic acid and ursodeoxycholic acid to yield lithocholic acid. Apparently the enzymes involved are relatively stereospecific since the 7 beta-hydroxy group of ursodeoxycholic acid was removed more slowly than the 7 alpha-hydroxy group of chenodeoxycholic acid.", "contents": "Transformation of chenodeoxycholic acid and ursodeoxycholic acid by human intestinal bacteria. Feces from normal subjects and patients with cerebrotendinous xanthomatosis were incubated anaerobically with labeled chenodeoxycholic acid and ursodeoxycholine acid for known periods, and the bile acids formed were analyzed by TLC and scintillation counting. In the normal subjects, 80% of the chenodeoxycholic acid and 41% of the ursodeoxycholic acid were 7-dehydroxylated to lithocholic acid during 2 hr of incubation. In contrast, the fecal flora of the CTX patients transformed only 5% of chenodeoxycholic acid and less than 1% of ursodeoxycholic acid to lithocholic acid during the same time period. In several subjects (normals and CTX), the intestinal flora converted chenodeoxycholic acid to ursodeoxycholic acid without the accumulation of the hypothetical intermediate 7-ketolithocholic acid (3 alpha-hydroxy-7-keto-5 beta-cholanoic acid). These results indicate that the fecal bacterial flora is capable of 7-dehydroxylating chenodeoxycholic acid and ursodeoxycholic acid to yield lithocholic acid. Apparently the enzymes involved are relatively stereospecific since the 7 beta-hydroxy group of ursodeoxycholic acid was removed more slowly than the 7 alpha-hydroxy group of chenodeoxycholic acid."}
{"id": "PMID:488635", "title": "Griseofulvin-induced cholestasis in Swiss albino mice.", "content": "Griseofulvin was fed to male Swiss albino mice, which were sacrificed at varying times after the initiation of the feeding. The following were compared with mice fed a control diet: hepatic histology, hepatic weight, plasma glycocholate, glycolithocholate, cholesterol, bilirubin, and alkaline phosphatase. Concurrent with the development of hepatic protoporphyria, a progressive cholestatic lesion was produced with marked bile canalicular dilatation and elevation of the plasma bile salts, alkaline phosphatase, and cholesterol without a rise in bilirubin. Adaptation to the cholestatic injury occurred in about 60 days despite continued griseofulvin feeding. This was evidenced by decreased values in the biochemical profile with concomitant improvement in the bile canalicular morphology. Following this event of adaptation, Mallory bodies began to appear in the livers, often in the periphery of the hepatic lobule. This model may be useful in studying mechanisms of cholestasis, Mallory body formation, and their relationship to altered microtubular systems in the hepatocyte.", "contents": "Griseofulvin-induced cholestasis in Swiss albino mice. Griseofulvin was fed to male Swiss albino mice, which were sacrificed at varying times after the initiation of the feeding. The following were compared with mice fed a control diet: hepatic histology, hepatic weight, plasma glycocholate, glycolithocholate, cholesterol, bilirubin, and alkaline phosphatase. Concurrent with the development of hepatic protoporphyria, a progressive cholestatic lesion was produced with marked bile canalicular dilatation and elevation of the plasma bile salts, alkaline phosphatase, and cholesterol without a rise in bilirubin. Adaptation to the cholestatic injury occurred in about 60 days despite continued griseofulvin feeding. This was evidenced by decreased values in the biochemical profile with concomitant improvement in the bile canalicular morphology. Following this event of adaptation, Mallory bodies began to appear in the livers, often in the periphery of the hepatic lobule. This model may be useful in studying mechanisms of cholestasis, Mallory body formation, and their relationship to altered microtubular systems in the hepatocyte."}
{"id": "PMID:488636", "title": "Percutaneous pancreatography: case report and presentation of technique.", "content": "A case of traumatic pancreatitis with a radiopaque calculus producing pain by obstructing the distal pancreatic duct is presented. Preoperative ductal anatomy was defined by a percutaneous pancreatogram that established the presence of mechanical duct obstruction as the cause of pain, and the potential for operative relief of duct obstruction. A remission from pain resulted from pancreatic duct decompression by a lateral pancreaticojejunostomy. The potential value of percutaneous pancreatography is discussed.", "contents": "Percutaneous pancreatography: case report and presentation of technique. A case of traumatic pancreatitis with a radiopaque calculus producing pain by obstructing the distal pancreatic duct is presented. Preoperative ductal anatomy was defined by a percutaneous pancreatogram that established the presence of mechanical duct obstruction as the cause of pain, and the potential for operative relief of duct obstruction. A remission from pain resulted from pancreatic duct decompression by a lateral pancreaticojejunostomy. The potential value of percutaneous pancreatography is discussed."}
{"id": "PMID:488637", "title": "99mTechnetium pyridoxylidene glutamate imaging in visceral heterotaxy (Ivemark's syndrome).", "content": "A two and one-half year old boy with complex congenital heart disease had Howell-Jolly bodies in his blood raising the possibility of the asplenia syndrome. A 99mTc-sulfur colloid liver-spleen scan was reported normal, but the right lobe of the abnormally reversed liver was mistaken for the spleen. The diagnosis of visceral heterotaxy (Ivemark's syndrome) was established after scanning the patient with a new radiopharmaceutical, 99mTechnetium pyridoxylidene glutamate. The agent clearly demonstrated a left-sided gall bladder, and on comparison with the sulfur colloid scan it was established that asplenia was present. Radionuclide imaging with the new generation of hepatobiliary agents is a reliable method to document asplenia and is useful in studying patients with visceral heterotaxy.", "contents": "99mTechnetium pyridoxylidene glutamate imaging in visceral heterotaxy (Ivemark's syndrome). A two and one-half year old boy with complex congenital heart disease had Howell-Jolly bodies in his blood raising the possibility of the asplenia syndrome. A 99mTc-sulfur colloid liver-spleen scan was reported normal, but the right lobe of the abnormally reversed liver was mistaken for the spleen. The diagnosis of visceral heterotaxy (Ivemark's syndrome) was established after scanning the patient with a new radiopharmaceutical, 99mTechnetium pyridoxylidene glutamate. The agent clearly demonstrated a left-sided gall bladder, and on comparison with the sulfur colloid scan it was established that asplenia was present. Radionuclide imaging with the new generation of hepatobiliary agents is a reliable method to document asplenia and is useful in studying patients with visceral heterotaxy."}
{"id": "PMID:488638", "title": "Diagnosis of annular pancreas with endoscopic retrograde cholangiopancreatography.", "content": "Two patients with annular pancreas are described. The diagnosis was established unequivocally with endoscopic retrograde cholangiopancreatography before operation. In both patients there was pancreatitis of the annular pancreas. The first patient also had congenital absence of the ventral pancreas and pancreatic insufficiency. The second patient subsequently developed gastric outlet obstruction. The literature is reviewed.", "contents": "Diagnosis of annular pancreas with endoscopic retrograde cholangiopancreatography. Two patients with annular pancreas are described. The diagnosis was established unequivocally with endoscopic retrograde cholangiopancreatography before operation. In both patients there was pancreatitis of the annular pancreas. The first patient also had congenital absence of the ventral pancreas and pancreatic insufficiency. The second patient subsequently developed gastric outlet obstruction. The literature is reviewed."}
{"id": "PMID:488649", "title": "Effect of water and fat on gastric emptying of solid meals.", "content": "Gastric emptying of solid egg meals tagged with 99mtechnetium sulfur colloid was studied in 10 normal subjects and in 6 patients after truncal vagotomy, antrectomy, and gastrojejunostomy (Billroth II) for peptic ulcer disease. Technetium sulfur colloid was found to bind more firmly to cooked egg whites than did the water soluble marker DTPA. Water accelerated gastric emptying in Billroth II patients to a greater degree than in normal subjects, in whom the effect of water was minimal and transient. Fat inhibited solid emptying both in normals and in Billroth II patients. These findings suggest that in humans there are jejunal receptors for fat that may inhibit gastric emptying even following truncal vagotomy.", "contents": "Effect of water and fat on gastric emptying of solid meals. Gastric emptying of solid egg meals tagged with 99mtechnetium sulfur colloid was studied in 10 normal subjects and in 6 patients after truncal vagotomy, antrectomy, and gastrojejunostomy (Billroth II) for peptic ulcer disease. Technetium sulfur colloid was found to bind more firmly to cooked egg whites than did the water soluble marker DTPA. Water accelerated gastric emptying in Billroth II patients to a greater degree than in normal subjects, in whom the effect of water was minimal and transient. Fat inhibited solid emptying both in normals and in Billroth II patients. These findings suggest that in humans there are jejunal receptors for fat that may inhibit gastric emptying even following truncal vagotomy."}
{"id": "PMID:488697", "title": "Infant visual habituation as a function of exposure time and stimulus similarity.", "content": "The course of visual response integration was examined in four-month-old infants (N = 32 boys and girls) by presenting a bull's eye pattern pair and a striped pattern pair on a single display board. On the basis of Jeffrey's serial habituation hypothesis it was predicted that in the early period of exposure the infant's scanning behavior would be limited to two like pairs, but that as attention waned to the similar pairs one or more of the less preferred stimuli would be integrated into the attending response. A measure of three consecutive looks indicated that attention confined to one similar pair decreased significantly over time, whereas the initially lower three-look sequences to two similar and one dissimilar stimuli increased significantly.", "contents": "Infant visual habituation as a function of exposure time and stimulus similarity. The course of visual response integration was examined in four-month-old infants (N = 32 boys and girls) by presenting a bull's eye pattern pair and a striped pattern pair on a single display board. On the basis of Jeffrey's serial habituation hypothesis it was predicted that in the early period of exposure the infant's scanning behavior would be limited to two like pairs, but that as attention waned to the similar pairs one or more of the less preferred stimuli would be integrated into the attending response. A measure of three consecutive looks indicated that attention confined to one similar pair decreased significantly over time, whereas the initially lower three-look sequences to two similar and one dissimilar stimuli increased significantly."}
{"id": "PMID:488698", "title": "Detecting mutations expressed during early development of cultured mammalian embryos.", "content": "Mammalian embryo culture systems can be used to study dominant lethal, recessive lethal, and visible mutations that are expressed between fertilization and early stages of organogenesis. The incidence of dominant lethal mutations has been determined for cultured mouse embryos by morphological observations during pre-implantation and early post-implantation growth in vitro, and the mechanisms of dominant lethal mutations have been studied cytogenetically. Recessive lethal mutations have been studied with cultured embryos, although they can be initially detected only with appropriate breeding protocols. Visible mutations that are due to deletions or single-site base alterations in the DNA can be detected in embryos by isoelectric focusing and gel-electrophoresis techniques.--The principal advantage of cultured embryos for detecting mutations and studying the mechanism of action of particular mutagens is that embryos are accessible for analysis before death with a minimum of indirect maternal effects. The primary disadvantages, which may be alleviated with improved culture conditions, are that only a limited amount of tissue is available, attrition and retardation occur, and offspring are not recovered for further breeding studies.", "contents": "Detecting mutations expressed during early development of cultured mammalian embryos. Mammalian embryo culture systems can be used to study dominant lethal, recessive lethal, and visible mutations that are expressed between fertilization and early stages of organogenesis. The incidence of dominant lethal mutations has been determined for cultured mouse embryos by morphological observations during pre-implantation and early post-implantation growth in vitro, and the mechanisms of dominant lethal mutations have been studied cytogenetically. Recessive lethal mutations have been studied with cultured embryos, although they can be initially detected only with appropriate breeding protocols. Visible mutations that are due to deletions or single-site base alterations in the DNA can be detected in embryos by isoelectric focusing and gel-electrophoresis techniques.--The principal advantage of cultured embryos for detecting mutations and studying the mechanism of action of particular mutagens is that embryos are accessible for analysis before death with a minimum of indirect maternal effects. The primary disadvantages, which may be alleviated with improved culture conditions, are that only a limited amount of tissue is available, attrition and retardation occur, and offspring are not recovered for further breeding studies."}
{"id": "PMID:488699", "title": "Minor viability mutants in Drosophila.", "content": "Drosophila experiments have demonstrated that spontaneous mutants causing minor decreases in viability occur much more often than those causing drastic effects. They also have a much greater relative effect in heterozygotes, enough that the absolute heterozygous viability decrease is roughly the same for mild as for lethal mutants. On the other hand, with EMS treatment and especially with radiation, the mild mutants are less frequent relative to lethals.--I suggest that the failure to detect heterozygous effects on fitness components from multi-generation radiation experiments on mice due to the relative infrequency of mild effects and the relatively small heterozygous effect of drastic mutants. Chemicals that produce effects more like spontaneous mutations might produce quite different results in such experiments.", "contents": "Minor viability mutants in Drosophila. Drosophila experiments have demonstrated that spontaneous mutants causing minor decreases in viability occur much more often than those causing drastic effects. They also have a much greater relative effect in heterozygotes, enough that the absolute heterozygous viability decrease is roughly the same for mild as for lethal mutants. On the other hand, with EMS treatment and especially with radiation, the mild mutants are less frequent relative to lethals.--I suggest that the failure to detect heterozygous effects on fitness components from multi-generation radiation experiments on mice due to the relative infrequency of mild effects and the relatively small heterozygous effect of drastic mutants. Chemicals that produce effects more like spontaneous mutations might produce quite different results in such experiments."}
{"id": "PMID:488700", "title": "Comments on mutagenesis risk estimation.", "content": "Several hypotheses and concepts have tended to oversimplify the problem of mutagenesis and can be misleading when used for genetic risk estimation. These include: (1) the hypothesis that radiation-induced mutation frequency depends primarily on the DNA content per haploid genome, (2) the extension of this concept to chemical mutagenesis, (3) the view that, since \"DNA is DNA,\" mutational effects can be expected to be qualitatively similar in all organisms, (4) the REC unit, and (5) the view that mutation rates from chronic irradiation can be theoretically and accurately predicted from acute irradiation data. Therefore, direct determination of frequencies of transmitted mutations in mammals continues to be important for risk estimation, and the specific-locus method in mice is shown to be not as expensive as is commonly supposed for many of the chemical testing requirements.", "contents": "Comments on mutagenesis risk estimation. Several hypotheses and concepts have tended to oversimplify the problem of mutagenesis and can be misleading when used for genetic risk estimation. These include: (1) the hypothesis that radiation-induced mutation frequency depends primarily on the DNA content per haploid genome, (2) the extension of this concept to chemical mutagenesis, (3) the view that, since \"DNA is DNA,\" mutational effects can be expected to be qualitatively similar in all organisms, (4) the REC unit, and (5) the view that mutation rates from chronic irradiation can be theoretically and accurately predicted from acute irradiation data. Therefore, direct determination of frequencies of transmitted mutations in mammals continues to be important for risk estimation, and the specific-locus method in mice is shown to be not as expensive as is commonly supposed for many of the chemical testing requirements."}
{"id": "PMID:488705", "title": "Mutations of Chinese hamster somatic cells from 2-deoxygalactose sensitivity to resistance.", "content": "In Chinese hamster somatic cells, the spontaneous change of phenotype from 2-deoxygalactose sensitivity to resistance was studied using fluctuation test experiments \u00e0 la Luria and Delbr\u00fcck (1943) for four Chinese hamster cell strains derived from V79. The results are consistent with true mutational events. The mutation rates are in the range of 1 to 3.5 X 10(-5) per cell per generation. The relationship between the 2-deoxyglactose resistance and the galactokinase markers is discussed.", "contents": "Mutations of Chinese hamster somatic cells from 2-deoxygalactose sensitivity to resistance. In Chinese hamster somatic cells, the spontaneous change of phenotype from 2-deoxygalactose sensitivity to resistance was studied using fluctuation test experiments \u00e0 la Luria and Delbr\u00fcck (1943) for four Chinese hamster cell strains derived from V79. The results are consistent with true mutational events. The mutation rates are in the range of 1 to 3.5 X 10(-5) per cell per generation. The relationship between the 2-deoxyglactose resistance and the galactokinase markers is discussed."}
{"id": "PMID:488706", "title": "Population genetics of Drosophila amylase. I. Genetic control of tissue-specific expression in D. pseudoobscura.", "content": "Drosophila pseudoobscura is polymorphic for tissue-specific expression of alpha-amylase in adult midguts. This enzyme is encoded by a single locus, Amy, on the third chromosome. In this paper we show: (1) Up to about 12 days post-eclosion, the midgut activity patterns remain stable; after 12 days areas not showing activity previously begin to show activity. Thus, the genes controlling the expression of Amy are temporally acting. (2) Diet affects the quantitative, but not the qualitative, expression of Amy. (3) The expression of Amy in adult midguts is under genetic control. Selection for different frequencies of patterns is possible; realized heritabilities are 0.20 to 0.50. Partial linkage with third chromosome inversions has been demonstrated; the genes or elements controlling Amy expression are not, however, confined to the third chromosome. (4) The genetic elements controlling tissue-specific expression of amylase do not coordinately control the expression of five other \"digestive-type\" enzymes that were studied.--This polymorphism appears to be analogous to that studied by Abraham and Doane (1978) in D. melanogaster, wherein they have mapped regulatory genes.", "contents": "Population genetics of Drosophila amylase. I. Genetic control of tissue-specific expression in D. pseudoobscura. Drosophila pseudoobscura is polymorphic for tissue-specific expression of alpha-amylase in adult midguts. This enzyme is encoded by a single locus, Amy, on the third chromosome. In this paper we show: (1) Up to about 12 days post-eclosion, the midgut activity patterns remain stable; after 12 days areas not showing activity previously begin to show activity. Thus, the genes controlling the expression of Amy are temporally acting. (2) Diet affects the quantitative, but not the qualitative, expression of Amy. (3) The expression of Amy in adult midguts is under genetic control. Selection for different frequencies of patterns is possible; realized heritabilities are 0.20 to 0.50. Partial linkage with third chromosome inversions has been demonstrated; the genes or elements controlling Amy expression are not, however, confined to the third chromosome. (4) The genetic elements controlling tissue-specific expression of amylase do not coordinately control the expression of five other \"digestive-type\" enzymes that were studied.--This polymorphism appears to be analogous to that studied by Abraham and Doane (1978) in D. melanogaster, wherein they have mapped regulatory genes."}
{"id": "PMID:488707", "title": "Population genetics of Drosophila amylase. II. Geographic patterns in D. pseudoobscura.", "content": "Morph frequencies of three related polymorphisms were determined in ten natural populations of Drosophila pseudoobscura. They are the well-known inversion polymorphism of the third chromosome and the polymorphism for alpha-amylase produced by the structural gene Amy (which resides on the third chromosome). The third polymorphism was for tissue-specific expression of Amy in adult midguts; a total of 13 different patterns of activity have been observed. The preceding paper (Powell and Lichtenfels 1979) reports evidence that the variation in Amy expression is under polygenic control. Here we show that the polymorphism for midgut patterns occurs in natural populations and is not an artifact of laboratory rearing.--From population to population, Amy allele frequencies and frequencies of inversions belonging to different phylads vary coordinately. The geographic variation in alpha-amylase midgut activity patterns is uncorrelated with that for the other two types of polymorphisms. Furthermore, no correlation was detected between activity pattern(s) and Amy genotype(s) when both were assayed in the same individual.--These results imply that whatever the evolutionary-ecological forces are that control frequencies of the structural gene variants, they are not the same factors that control the frequencies of polymorphic genetic factors responsible for the tissue-specific expression of the enzyme.", "contents": "Population genetics of Drosophila amylase. II. Geographic patterns in D. pseudoobscura. Morph frequencies of three related polymorphisms were determined in ten natural populations of Drosophila pseudoobscura. They are the well-known inversion polymorphism of the third chromosome and the polymorphism for alpha-amylase produced by the structural gene Amy (which resides on the third chromosome). The third polymorphism was for tissue-specific expression of Amy in adult midguts; a total of 13 different patterns of activity have been observed. The preceding paper (Powell and Lichtenfels 1979) reports evidence that the variation in Amy expression is under polygenic control. Here we show that the polymorphism for midgut patterns occurs in natural populations and is not an artifact of laboratory rearing.--From population to population, Amy allele frequencies and frequencies of inversions belonging to different phylads vary coordinately. The geographic variation in alpha-amylase midgut activity patterns is uncorrelated with that for the other two types of polymorphisms. Furthermore, no correlation was detected between activity pattern(s) and Amy genotype(s) when both were assayed in the same individual.--These results imply that whatever the evolutionary-ecological forces are that control frequencies of the structural gene variants, they are not the same factors that control the frequencies of polymorphic genetic factors responsible for the tissue-specific expression of the enzyme."}
{"id": "PMID:488708", "title": "Mutation rates, population sizes and amounts of electrophoretic variation of enzyme loci in natural populations.", "content": "A method is presented for estimating relative mutation rates or relative effective population sizes, under the hypothesis of adaptively neutral allelic variation. This method was applied to seven surveys of electrophoretic variation. It was observed that electrophoretic mutation rates so obtained follow the gamma distribution and, in Drosophila, are positively correlated with the molecular weights of the enzymes subunits. The variance in mutation rate is larger under the step-wise model of electrophoretic mutation than under the infinite-alleles model. Rates for the most variable loci may exceed rates for less variable loci by a factor of 500. For completely invariant loci, this factor may be as high as 4 X 10(4), an observation suggesting that these loci are subject to purifying selection. In contrast to mutation rates, effective population sizes may vary at the most by a factor of ten. These results support the hypothesis that differences in the amount of electrophoretic variability among polymorphic loci may reflect differences in the rate by which electrophoretically detectable variation is generated in population.", "contents": "Mutation rates, population sizes and amounts of electrophoretic variation of enzyme loci in natural populations. A method is presented for estimating relative mutation rates or relative effective population sizes, under the hypothesis of adaptively neutral allelic variation. This method was applied to seven surveys of electrophoretic variation. It was observed that electrophoretic mutation rates so obtained follow the gamma distribution and, in Drosophila, are positively correlated with the molecular weights of the enzymes subunits. The variance in mutation rate is larger under the step-wise model of electrophoretic mutation than under the infinite-alleles model. Rates for the most variable loci may exceed rates for less variable loci by a factor of 500. For completely invariant loci, this factor may be as high as 4 X 10(4), an observation suggesting that these loci are subject to purifying selection. In contrast to mutation rates, effective population sizes may vary at the most by a factor of ten. These results support the hypothesis that differences in the amount of electrophoretic variability among polymorphic loci may reflect differences in the rate by which electrophoretically detectable variation is generated in population."}
{"id": "PMID:488709", "title": "Maintenance of genetic variability under the pressure of neutral and deleterious mutations in a finite population.", "content": "In order to assess the effect of deleterious mutations on various measures of genic variation, approximate formulas have been developed for the frequency spectrum, the mean number of alleles in a sample, and the mean homozygosity; in some particular cases, exact formulas have been obtained. The assumptions made are that two classes of mutations exist, neutral and deleterious, and that selection is strong enough to keep deleterious alleles in low frequencies, the mode of selection being either genic or recessive. The main findings are: (1) If the expected value (q) of the sum of the frequencies of deleterious alleles is about 10% or less, then the presence of deleterious alleles causes only a minor reduction in the mean number of neutral alleles in a sample, as compared to the case of q = 0. Also, the low- and intermediate-frequency parts of the frequency spectrum of neutral alleles are little affected by the presence of deleterious alleles, though the high-frequency part may be changed drastically. (2) The contribution of deleterious mutations to the expected total number of alleles in a sample can be quite large even if q is only 1 or 2%. (3) The mean homozygosity is roughly equal to (1--2q)/(1 + theta 1), where theta 1 is twice the number of new neutral mutations occurring in each generation in the total population. Thus, deleterious mutations increase the mean heterozygosity by about 2q/(1 + theta 1). The present results have been applied to study the controversial problem of how deleterious mutations may affect the testing of the neutral mutation hypothesis.", "contents": "Maintenance of genetic variability under the pressure of neutral and deleterious mutations in a finite population. In order to assess the effect of deleterious mutations on various measures of genic variation, approximate formulas have been developed for the frequency spectrum, the mean number of alleles in a sample, and the mean homozygosity; in some particular cases, exact formulas have been obtained. The assumptions made are that two classes of mutations exist, neutral and deleterious, and that selection is strong enough to keep deleterious alleles in low frequencies, the mode of selection being either genic or recessive. The main findings are: (1) If the expected value (q) of the sum of the frequencies of deleterious alleles is about 10% or less, then the presence of deleterious alleles causes only a minor reduction in the mean number of neutral alleles in a sample, as compared to the case of q = 0. Also, the low- and intermediate-frequency parts of the frequency spectrum of neutral alleles are little affected by the presence of deleterious alleles, though the high-frequency part may be changed drastically. (2) The contribution of deleterious mutations to the expected total number of alleles in a sample can be quite large even if q is only 1 or 2%. (3) The mean homozygosity is roughly equal to (1--2q)/(1 + theta 1), where theta 1 is twice the number of new neutral mutations occurring in each generation in the total population. Thus, deleterious mutations increase the mean heterozygosity by about 2q/(1 + theta 1). The present results have been applied to study the controversial problem of how deleterious mutations may affect the testing of the neutral mutation hypothesis."}
{"id": "PMID:488710", "title": "A model allowing continuous variation in electrophoretic mobility of neutral alleles.", "content": "The infinite-sites model with no recombination is extended to include mutations that affect electrophoretic mobility. The model allows the effect of a single-site mutation to have a continuous effect on mobility. Formulae are obtained for the variance of electrophoretic mobility of alleles after an arbitrary lenght of time. A special case of the general model is the case of stepwise production of neutral alleles with an arbitrary number of steps.", "contents": "A model allowing continuous variation in electrophoretic mobility of neutral alleles. The infinite-sites model with no recombination is extended to include mutations that affect electrophoretic mobility. The model allows the effect of a single-site mutation to have a continuous effect on mobility. Formulae are obtained for the variance of electrophoretic mobility of alleles after an arbitrary lenght of time. A special case of the general model is the case of stepwise production of neutral alleles with an arbitrary number of steps."}
{"id": "PMID:488711", "title": "[Genetic studies of Photobacterium mandapamensis. II. The classification of mutants with an altered luminescence intensity according to their sensitivity to exogenous aldehyde].", "content": "The collection of 157 dark and dim Photobacterium mandapamensis strains was divided into four groups using the addition of 0.2 ml of 0.15% myristis aldehyde to cell suspension. The luminescence did not change in the presence of the aldehyde in 76 mutants, it decreased in 30 mutants, it was 2--8-fold increased in 35 mutants, and it was increased more than 10-fold in 16 mutant strains. 19 strains of those having luminescence in the presence of the aldehyde have mutations in genes controlling the biosynthesis of the aldehyde factor. Among them mutants are chosen which can be used as indicators for the aldehyde presence in the medium.", "contents": "[Genetic studies of Photobacterium mandapamensis. II. The classification of mutants with an altered luminescence intensity according to their sensitivity to exogenous aldehyde]. The collection of 157 dark and dim Photobacterium mandapamensis strains was divided into four groups using the addition of 0.2 ml of 0.15% myristis aldehyde to cell suspension. The luminescence did not change in the presence of the aldehyde in 76 mutants, it decreased in 30 mutants, it was 2--8-fold increased in 35 mutants, and it was increased more than 10-fold in 16 mutant strains. 19 strains of those having luminescence in the presence of the aldehyde have mutations in genes controlling the biosynthesis of the aldehyde factor. Among them mutants are chosen which can be used as indicators for the aldehyde presence in the medium."}
{"id": "PMID:488712", "title": "[Genetic classification of riboflavin-dependent mutants of Pichia guilliermondii yeasts].", "content": "114 riboflavinless mutants were selected from the genetic line of Pichia guilliermondii yeast. By means of accumulation test the mutants were divided into five biochemical groups. In genetic experiments seven complementation classes were found among 106 mutants. The strains of the I biochemical group, accumulating no specific products, corresponded to complementation class rib1; II group, accumulating 2,4,5-triaminopyrimidine - to the class rib2; III group, accumulating 2,6-dihydroxy-4-ribitylaminopyrimidine - to the class rib3; the mutants of the IV group, accumulating 2,6-dihydroxy-5-amino-4-ribitylaminopyrimidine, were divided into three complementation classes rib4, rib5 and rib6; the mutants of the V group, acculumating 6,7-dimethyl-8-ribityllumazine, corresponded to the class rib7. Two mutants of the IV biochemical group within complementation classes rib4 and rib5 were detected could not grow in the medium with diacetyl without riboflavin. Intragenic complementation was found within classes rib6 and rib7. No linkage between mutations of different complementation classes was detected.", "contents": "[Genetic classification of riboflavin-dependent mutants of Pichia guilliermondii yeasts]. 114 riboflavinless mutants were selected from the genetic line of Pichia guilliermondii yeast. By means of accumulation test the mutants were divided into five biochemical groups. In genetic experiments seven complementation classes were found among 106 mutants. The strains of the I biochemical group, accumulating no specific products, corresponded to complementation class rib1; II group, accumulating 2,4,5-triaminopyrimidine - to the class rib2; III group, accumulating 2,6-dihydroxy-4-ribitylaminopyrimidine - to the class rib3; the mutants of the IV group, accumulating 2,6-dihydroxy-5-amino-4-ribitylaminopyrimidine, were divided into three complementation classes rib4, rib5 and rib6; the mutants of the V group, acculumating 6,7-dimethyl-8-ribityllumazine, corresponded to the class rib7. Two mutants of the IV biochemical group within complementation classes rib4 and rib5 were detected could not grow in the medium with diacetyl without riboflavin. Intragenic complementation was found within classes rib6 and rib7. No linkage between mutations of different complementation classes was detected."}
{"id": "PMID:488713", "title": "[Cytogenetic study of spontaneous lympholeukemia in AKR mice in the process of its transplantation to an isogeneic line of animals].", "content": "Spontaneous leucosis was cytogenetically studied in subsequent generations (from 1 to 150) of AKR mice. By means of the differential staining technique of chromosomes, large variations in chromosome numbers were found in the karyotypes of leucotic cells of different generations, and the formation of cell clones containing different marker chromosomes as well as the dominance of a hyperdiploid clone with 41-42 chromosomes was revealed. Chromosome analysis of such hyperdiploid cells of the 150th generation has indicated that the supernumerary chromosomes (in 88.0% of cases examined) belong to the smallest chromosomes of the mouse karyogramm (to the 18-19th chromosome pairs or to chromosomes smaller than those of 19th pair). Similar trisomy was also observed in hypodiploid and pseudodiploid leucosis cells. It is suggested that the cell clone with trisomy for the smallest chromosomes is specific to the spontaneous lympholeucosis in AKR mice as well as to the leucosis transplanted to isogenic mice for a number of subsequent generations. Increased rate of hyperdiploid cells was associated with a generalization of leucosis. It was concluded that the development rate and the severity of transplanted lympholeucosis in AKR mice was determined by the domination of the cell clone with trisomy for the 18-19th chromosome pairs in the population of leucotic cells.", "contents": "[Cytogenetic study of spontaneous lympholeukemia in AKR mice in the process of its transplantation to an isogeneic line of animals]. Spontaneous leucosis was cytogenetically studied in subsequent generations (from 1 to 150) of AKR mice. By means of the differential staining technique of chromosomes, large variations in chromosome numbers were found in the karyotypes of leucotic cells of different generations, and the formation of cell clones containing different marker chromosomes as well as the dominance of a hyperdiploid clone with 41-42 chromosomes was revealed. Chromosome analysis of such hyperdiploid cells of the 150th generation has indicated that the supernumerary chromosomes (in 88.0% of cases examined) belong to the smallest chromosomes of the mouse karyogramm (to the 18-19th chromosome pairs or to chromosomes smaller than those of 19th pair). Similar trisomy was also observed in hypodiploid and pseudodiploid leucosis cells. It is suggested that the cell clone with trisomy for the smallest chromosomes is specific to the spontaneous lympholeucosis in AKR mice as well as to the leucosis transplanted to isogenic mice for a number of subsequent generations. Increased rate of hyperdiploid cells was associated with a generalization of leucosis. It was concluded that the development rate and the severity of transplanted lympholeucosis in AKR mice was determined by the domination of the cell clone with trisomy for the 18-19th chromosome pairs in the population of leucotic cells."}
{"id": "PMID:488714", "title": "[Possibilities of the clinical comparison of diseases with a hereditary burden in the descendant generation using the model of \"parents-children\" ill with schizophrenia].", "content": "A special statistic method of confrontation of diseases with aggravation hereditary in the group \"parents - children\" is proposed. This method can be used under clinical formalization of the diseases. It is shown on a model group parents - children (118 pairs) suffering with schizophrenia, that statistical confrontation makes possible to work out group and individual prognoses in the descending generation. It is found that invariability in the descending generation is provided by a small number of stable indices, other symptoms being variable. Statistic description of the disease symptoms in the descending generation is of interest for planning genetic interpretation.", "contents": "[Possibilities of the clinical comparison of diseases with a hereditary burden in the descendant generation using the model of \"parents-children\" ill with schizophrenia]. A special statistic method of confrontation of diseases with aggravation hereditary in the group \"parents - children\" is proposed. This method can be used under clinical formalization of the diseases. It is shown on a model group parents - children (118 pairs) suffering with schizophrenia, that statistical confrontation makes possible to work out group and individual prognoses in the descending generation. It is found that invariability in the descending generation is provided by a small number of stable indices, other symptoms being variable. Statistic description of the disease symptoms in the descending generation is of interest for planning genetic interpretation."}
{"id": "PMID:488715", "title": "[Cytogenetic changes in the bone marrow cells of long-term irradiated rats].", "content": "The occurrence and characteristics of the chromosome structural changes in femur bone marrow cells under continuous irradiation with the exposure rate of 50 R/day within 90 days was followed. The 25% increase in the chromosome aberration frequency was observed within 7 days of the irradiation, and then the aberration rate was constant up to the end of the irradiation (90 days).", "contents": "[Cytogenetic changes in the bone marrow cells of long-term irradiated rats]. The occurrence and characteristics of the chromosome structural changes in femur bone marrow cells under continuous irradiation with the exposure rate of 50 R/day within 90 days was followed. The 25% increase in the chromosome aberration frequency was observed within 7 days of the irradiation, and then the aberration rate was constant up to the end of the irradiation (90 days)."}
{"id": "PMID:488746", "title": "Age dependent changes in the metabolism of 3H-thymidine and 3H-uridine in young rats.", "content": "The metabolism of subcutaneously injected 3H-thymidine and 3H-uridine was studied in 5, 15 and 30-day-old rats and some age-dependent differences were found. The total availability of 3H-uridine was found to decrease when the rats became older. In the case of 3H-thymidine, however, there was only minor differences in the total availability. The catabolism of both 3H-thymidine and 3H-uridine, as measured by the appearance of 3H-water, was more efficient when the rats became older. In the case of 3H-thymidine, however, the increased catabolic capacity seems to be compensated by a decreased proliferative growth so the total availability will be constant. It was also observed that the amount of intermediate catabolic products in the blood was much larger after an injection of 3H-uridine than after 3H-thymidine.", "contents": "Age dependent changes in the metabolism of 3H-thymidine and 3H-uridine in young rats. The metabolism of subcutaneously injected 3H-thymidine and 3H-uridine was studied in 5, 15 and 30-day-old rats and some age-dependent differences were found. The total availability of 3H-uridine was found to decrease when the rats became older. In the case of 3H-thymidine, however, there was only minor differences in the total availability. The catabolism of both 3H-thymidine and 3H-uridine, as measured by the appearance of 3H-water, was more efficient when the rats became older. In the case of 3H-thymidine, however, the increased catabolic capacity seems to be compensated by a decreased proliferative growth so the total availability will be constant. It was also observed that the amount of intermediate catabolic products in the blood was much larger after an injection of 3H-uridine than after 3H-thymidine."}
{"id": "PMID:488747", "title": "Growth rate and feed intake of selected and nonselected broilers.", "content": "The relative growth rates and feed intake patterns of selected and nonselected broiler populations were investigated in three trials. The relative growth rate of the selected populations was approximately twice the relative growth rate of the nonselected population during week 1, whereas by week 4 the rates of the selected and nonselected populations were similar. The percentage deviation between populations in feed intake was higher than the percentage deviation in body weights for week 1 in all trials. Feed intake strongly influenced the growth rate of selected and nonselected populations, especially from 3 to 8 weeks of age. The selected populations had superior feed efficiency from 0 to 4 weeks compared to the nonselected population, however, differences between populations from 4 to 8 weeks were not significant.", "contents": "Growth rate and feed intake of selected and nonselected broilers. The relative growth rates and feed intake patterns of selected and nonselected broiler populations were investigated in three trials. The relative growth rate of the selected populations was approximately twice the relative growth rate of the nonselected population during week 1, whereas by week 4 the rates of the selected and nonselected populations were similar. The percentage deviation between populations in feed intake was higher than the percentage deviation in body weights for week 1 in all trials. Feed intake strongly influenced the growth rate of selected and nonselected populations, especially from 3 to 8 weeks of age. The selected populations had superior feed efficiency from 0 to 4 weeks compared to the nonselected population, however, differences between populations from 4 to 8 weeks were not significant."}
{"id": "PMID:488748", "title": "Availability time and tissue utilization of exogenous L-fucose in the adult newt.", "content": "3H-L-fucose can be detected in the plasma of intraperitoneally injected adult newts as early as 3 minutes, peaks around 4 hours, and persists in low levels up to 48 hours. Studies of fucose levels in the tissues examined suggest a relatively short life span for many of the fucose-containing glycoproteins.", "contents": "Availability time and tissue utilization of exogenous L-fucose in the adult newt. 3H-L-fucose can be detected in the plasma of intraperitoneally injected adult newts as early as 3 minutes, peaks around 4 hours, and persists in low levels up to 48 hours. Studies of fucose levels in the tissues examined suggest a relatively short life span for many of the fucose-containing glycoproteins."}
{"id": "PMID:488749", "title": "Comparative findings on body size of children and youths living at urban centers and in rural areas.", "content": "The focus of this paper is on rural-urban differences in human body size during the period between ages 8 years and 15 years. Groups of village and city residents are compared on standing height, body weight, and other somatic dimensions. Wide variation is found: from data collected during 1955--1972, urban children and youths in Costa Rica, Finland, Jamaica, Lithuania, Poland, Romania, and South Africa are larger than their rural coevals by more than 3.0 cm in standing height, and 2.0 kg in body weight; while from measures taken during 1889--1910, urban children and youths in East Germany and England are smaller than their rural peers by 1.2 cm to 2.3 cm in standing height, and 0.6 kg to 0.8 kg in body weight. Intermediate differences characterize groups in Bulgaria, France, Ghana, Italy, Japan, Surinam, the Soviet Union, and the United States.", "contents": "Comparative findings on body size of children and youths living at urban centers and in rural areas. The focus of this paper is on rural-urban differences in human body size during the period between ages 8 years and 15 years. Groups of village and city residents are compared on standing height, body weight, and other somatic dimensions. Wide variation is found: from data collected during 1955--1972, urban children and youths in Costa Rica, Finland, Jamaica, Lithuania, Poland, Romania, and South Africa are larger than their rural coevals by more than 3.0 cm in standing height, and 2.0 kg in body weight; while from measures taken during 1889--1910, urban children and youths in East Germany and England are smaller than their rural peers by 1.2 cm to 2.3 cm in standing height, and 0.6 kg to 0.8 kg in body weight. Intermediate differences characterize groups in Bulgaria, France, Ghana, Italy, Japan, Surinam, the Soviet Union, and the United States."}
{"id": "PMID:488750", "title": "Enzyme activities and morphological appearance in functioning and excluded segments of the small intestine after shunt operation for obesity.", "content": "Five patients in whom small-intestinal bypass was performed for severe obesity had a second operation 11-19 months later because of insufficient weight loss. Mucosal enzyme activities and histological appearance were investigated in biopsies from different parts of the functioning and excluded small intestine. These were compared with biopsies form corresponding sites obtained at the first operation. In addition to a prominent increase in length, circumference, and mucosal thickness in the functioning shunt, the disaccharidases and two intracellular beta-galactosidases increased in specific activity,, especially in the distal ileal part of the shunt. In the excluded segment of the small intestine different enzymes showed a different response: trehalase increased and alkaline phosphate decreased significantly. Other enzymes that were measured showed a varied pattern. The results indicated that not only the luminal content but also other, presumably hormonal, factors regulated the enzyme activities, and that different regulating factors influenced the various enzymes differently. The marked adaptive increase in mucosal surface of the functioning shunt could be one factor in explaining the weight stabilisation and, in some cases, weight increase after the initial rapid weight loss after the operation for small-intestinal bypass. The increase in specific enzyme activities would further increase the digestive capacity of the shunt.", "contents": "Enzyme activities and morphological appearance in functioning and excluded segments of the small intestine after shunt operation for obesity. Five patients in whom small-intestinal bypass was performed for severe obesity had a second operation 11-19 months later because of insufficient weight loss. Mucosal enzyme activities and histological appearance were investigated in biopsies from different parts of the functioning and excluded small intestine. These were compared with biopsies form corresponding sites obtained at the first operation. In addition to a prominent increase in length, circumference, and mucosal thickness in the functioning shunt, the disaccharidases and two intracellular beta-galactosidases increased in specific activity,, especially in the distal ileal part of the shunt. In the excluded segment of the small intestine different enzymes showed a different response: trehalase increased and alkaline phosphate decreased significantly. Other enzymes that were measured showed a varied pattern. The results indicated that not only the luminal content but also other, presumably hormonal, factors regulated the enzyme activities, and that different regulating factors influenced the various enzymes differently. The marked adaptive increase in mucosal surface of the functioning shunt could be one factor in explaining the weight stabilisation and, in some cases, weight increase after the initial rapid weight loss after the operation for small-intestinal bypass. The increase in specific enzyme activities would further increase the digestive capacity of the shunt."}
{"id": "PMID:488751", "title": "Effects of lysine vasopressin and glypressin on the fibrinolytic system in cirrhosis.", "content": "In eight patients with cirrhosis of the liver and portal hypertension an intravenous infusion of lysine vasopressin induced a rapid increase in the plasma level of the fibrinolytic proenzyme plasminogen activator. In contrast, triglycyl lysine vasopressin (glypressin; GVP), in a dose known to lower portal venous pressure, produced no fibrinolytic response. This lack of fibrinolytic response represents an advantage of GVP over lysine vasopressin in addition to its longer in vivo half-life and lower cardiotoxicity. Clinical trials of GVP in the treatment of bleeding oesophageal varices are needed.", "contents": "Effects of lysine vasopressin and glypressin on the fibrinolytic system in cirrhosis. In eight patients with cirrhosis of the liver and portal hypertension an intravenous infusion of lysine vasopressin induced a rapid increase in the plasma level of the fibrinolytic proenzyme plasminogen activator. In contrast, triglycyl lysine vasopressin (glypressin; GVP), in a dose known to lower portal venous pressure, produced no fibrinolytic response. This lack of fibrinolytic response represents an advantage of GVP over lysine vasopressin in addition to its longer in vivo half-life and lower cardiotoxicity. Clinical trials of GVP in the treatment of bleeding oesophageal varices are needed."}
{"id": "PMID:488752", "title": "In vitro metabolism of creatinine, methylamine and amino acids by intestinal contents of normal and uraemic subjects.", "content": "An original method which uses in vitro anaerobic incubation at 37 degrees C followed by centrifugation, ultrafiltration, and ion exchange chromatography is described; it shows that faecal material suspended in physiological saline can destroy added creatinine. The rate of breakdown by suspensions from uraemic subjects (mean 780 mumol h-1kg-1 SEM 70) was slightly faster than in normal subjects (mean 550 mumol h-1kg-1 SEM 80). Methylamine concentration increased over eight hours as creatinine was metabolised and sarcosine appeared as an intermediate. The rates of these reactions varied within and between individuals and were inhibited by oxygen and centrifugation but not by oxytetracycline. Concentrations of free amino acids did not change significantly despite the formation of ammonia. This approach should be useful in studying the metabolic inter-relationships between intestinal contents and the host organism in health and disease.", "contents": "In vitro metabolism of creatinine, methylamine and amino acids by intestinal contents of normal and uraemic subjects. An original method which uses in vitro anaerobic incubation at 37 degrees C followed by centrifugation, ultrafiltration, and ion exchange chromatography is described; it shows that faecal material suspended in physiological saline can destroy added creatinine. The rate of breakdown by suspensions from uraemic subjects (mean 780 mumol h-1kg-1 SEM 70) was slightly faster than in normal subjects (mean 550 mumol h-1kg-1 SEM 80). Methylamine concentration increased over eight hours as creatinine was metabolised and sarcosine appeared as an intermediate. The rates of these reactions varied within and between individuals and were inhibited by oxygen and centrifugation but not by oxytetracycline. Concentrations of free amino acids did not change significantly despite the formation of ammonia. This approach should be useful in studying the metabolic inter-relationships between intestinal contents and the host organism in health and disease."}
{"id": "PMID:488753", "title": "Value of sigmoidoscopy and biopsy in detection of carcinoma and premalignant change in ulcerative colitis.", "content": "Of 111 carcinomas developing in 73 patients with ulcerative colitis, 46 (41.5%) arose in the rectum where they are directly accessible to sigmoidoscopy. Fifty-eight per cent of single carcinomas developed in the rectum. The extent and frequency of rectal dysplasia was assessed by examining slides of rectal mucosa with an eyepiece micrometer. Slides from 46 patients with carcinoma and 22 patients with dysplasia but no carcinoma in whom proctectomy or proctocolectomy had been carried out were examined by this method. Thirteen of 15 patients with carcinoma of the colon (87%) and 21/22 patients (95%) with large bowel dysplasia showed evidence only of rectal dysplasia. However, there was marked variability in the proportion of dysplastic rectal mucosa even in those patients with rectal carcinoma, while in some patients dysplasia was limited to a small focus. Because of the possibility of false negative biopsies due to sampling error, multiple biopsies should be taken to detect dysplasia. Their state should be recorded and deliberately varied at subsequent visits. Careful sigmoidoscopy and multiple biopsies in this study had potential value as an aid in the detection of 85-90% of all carcinomas. In practice the figure would almost certainly be lower due to intrinsic bias (see discussion) so that, although regular sigmoidoscopy and biopsy would be of great value when colonoscopy is not available, the latter should be included in any long-term programme of carcinoma prevention.", "contents": "Value of sigmoidoscopy and biopsy in detection of carcinoma and premalignant change in ulcerative colitis. Of 111 carcinomas developing in 73 patients with ulcerative colitis, 46 (41.5%) arose in the rectum where they are directly accessible to sigmoidoscopy. Fifty-eight per cent of single carcinomas developed in the rectum. The extent and frequency of rectal dysplasia was assessed by examining slides of rectal mucosa with an eyepiece micrometer. Slides from 46 patients with carcinoma and 22 patients with dysplasia but no carcinoma in whom proctectomy or proctocolectomy had been carried out were examined by this method. Thirteen of 15 patients with carcinoma of the colon (87%) and 21/22 patients (95%) with large bowel dysplasia showed evidence only of rectal dysplasia. However, there was marked variability in the proportion of dysplastic rectal mucosa even in those patients with rectal carcinoma, while in some patients dysplasia was limited to a small focus. Because of the possibility of false negative biopsies due to sampling error, multiple biopsies should be taken to detect dysplasia. Their state should be recorded and deliberately varied at subsequent visits. Careful sigmoidoscopy and multiple biopsies in this study had potential value as an aid in the detection of 85-90% of all carcinomas. In practice the figure would almost certainly be lower due to intrinsic bias (see discussion) so that, although regular sigmoidoscopy and biopsy would be of great value when colonoscopy is not available, the latter should be included in any long-term programme of carcinoma prevention."}
{"id": "PMID:488754", "title": "Radio-opaque pellets as faecal markers for faecal fat estimation in malabsorption.", "content": "Thirty-seven patients undergoing routine investigation for malabsorption were given radio-opaque pellets (ROP) which were compared with polyethylene glycol and chromic oxide as continuous faecal markers. Percentage recovery of all markers showed a wide range. The recovery of all markers was very similar, and radio-opaque pellet-corrected daily faecal fat correlated very closely with PEG-and chromic oxide-corrected fat results. Estimation of ROP is precise, simple, and minimises faecal handling. ROP are suitable for routine use in faecal fat studies and have advantages over markers used at present.", "contents": "Radio-opaque pellets as faecal markers for faecal fat estimation in malabsorption. Thirty-seven patients undergoing routine investigation for malabsorption were given radio-opaque pellets (ROP) which were compared with polyethylene glycol and chromic oxide as continuous faecal markers. Percentage recovery of all markers showed a wide range. The recovery of all markers was very similar, and radio-opaque pellet-corrected daily faecal fat correlated very closely with PEG-and chromic oxide-corrected fat results. Estimation of ROP is precise, simple, and minimises faecal handling. ROP are suitable for routine use in faecal fat studies and have advantages over markers used at present."}
{"id": "PMID:488755", "title": "Correlation of salivary and gastric acid secretions in duodenal ulcer patients in tropics.", "content": "Salivary flow rates on mechanical stimulation by forced spitting method and by chemical stimulation with 10% citric acid and gastric acidity using an augmented histamine test were determined in 20 adult patients suffering from duodenal ulcer and in 20 adult control subjects matched with respect to age, sex, and body weight. Salivary flow rates were found to be much higher in response to chemical than to mechanical stimulus in both the groups. Duodenal ulcer patients exhibited an unexplained exaggerated response to chemical stimulation. Salivary pH, amylase, sodium, and potassium levels showed no significant differences between the two groups. The flow rates by either method generally showed a positive correlation with body weight in both the groups. Histamine stimulated gastric acid secretion was higher in duodenal ulcer patients than in controls. Acid secretion did not appear to be related to weight and also showed no consistent correlation with the salivary flow rates. It was concluded that (1) the salivary flow was dependent on body weight in duodenal ulcer patients as well as in controls, and (2) although salivary gland hyperplasia could be postulated in duodenal ulcer patients on the basis of increased salivary flow, the latter was poorly related to maximal acid secretion and therefore, if a combination of parietal cell and salivary gland hyperplasia did exist, it should be considered as incidental.", "contents": "Correlation of salivary and gastric acid secretions in duodenal ulcer patients in tropics. Salivary flow rates on mechanical stimulation by forced spitting method and by chemical stimulation with 10% citric acid and gastric acidity using an augmented histamine test were determined in 20 adult patients suffering from duodenal ulcer and in 20 adult control subjects matched with respect to age, sex, and body weight. Salivary flow rates were found to be much higher in response to chemical than to mechanical stimulus in both the groups. Duodenal ulcer patients exhibited an unexplained exaggerated response to chemical stimulation. Salivary pH, amylase, sodium, and potassium levels showed no significant differences between the two groups. The flow rates by either method generally showed a positive correlation with body weight in both the groups. Histamine stimulated gastric acid secretion was higher in duodenal ulcer patients than in controls. Acid secretion did not appear to be related to weight and also showed no consistent correlation with the salivary flow rates. It was concluded that (1) the salivary flow was dependent on body weight in duodenal ulcer patients as well as in controls, and (2) although salivary gland hyperplasia could be postulated in duodenal ulcer patients on the basis of increased salivary flow, the latter was poorly related to maximal acid secretion and therefore, if a combination of parietal cell and salivary gland hyperplasia did exist, it should be considered as incidental."}
{"id": "PMID:488756", "title": "Grey scale ultrasound in Crohn's disease.", "content": "Grey scale ultrasound examination was performed in 20 patients with Crohn's disease of varying extent and severity. In 15 of the 20 patients studied, thickening of the wall of the terminal ileum and caecum, with adjoining inflammatory changes in the mesentery, produced recognisable patterns on longitudinal and transverse ultrasonographs. Grey scale ultrasound, although not providing the specificity or detail of conventional radiology, can be helpful in the assessment of ileocaecal Crohn's disease, particularly in younger patients where the radiation hazards of repeated x-ray examinations are undesirable.", "contents": "Grey scale ultrasound in Crohn's disease. Grey scale ultrasound examination was performed in 20 patients with Crohn's disease of varying extent and severity. In 15 of the 20 patients studied, thickening of the wall of the terminal ileum and caecum, with adjoining inflammatory changes in the mesentery, produced recognisable patterns on longitudinal and transverse ultrasonographs. Grey scale ultrasound, although not providing the specificity or detail of conventional radiology, can be helpful in the assessment of ileocaecal Crohn's disease, particularly in younger patients where the radiation hazards of repeated x-ray examinations are undesirable."}
{"id": "PMID:488757", "title": "Antipyrine clearance per unit volume liver: an assessment of hepatic function in chronic liver disease.", "content": "Liver size has been estimated clinically and by a non-invasive ultrasound technique in 16 normal subjects, 16 patients with cirrhosis, 10 patients with chronic biliary obstruction, and three patients with primary hepatoma. Antipyrine disposition was also measured in each subject. Hepatomegaly was not clinically detectable until there was approximately a 20% increase in liver size. Additional increases in size correlated significantly with clinical estimates of hepatomegaly. Antipyrine clearance had a three-fold range in normal subjects. Its mean value was significantly reduced in each subgroup of patients with liver disease. However, 48% of patients with liver disease had values within the normal range. In normal subjects there was a significant correlation between antipyrine clearance and liver volume. Thus, intersubject variation in clearance normalised for liver volume was less than clearance alone. Antipyrine clearance normalised for liver volume in patients with liver disease was significantly lower than in normal subjects and there was no overlap with normal subjects. In conclusion, assessment of drug metabolising efficiency per unit volume of liver increased the discrimination in differentiating subjects with normal from abnormal livers.", "contents": "Antipyrine clearance per unit volume liver: an assessment of hepatic function in chronic liver disease. Liver size has been estimated clinically and by a non-invasive ultrasound technique in 16 normal subjects, 16 patients with cirrhosis, 10 patients with chronic biliary obstruction, and three patients with primary hepatoma. Antipyrine disposition was also measured in each subject. Hepatomegaly was not clinically detectable until there was approximately a 20% increase in liver size. Additional increases in size correlated significantly with clinical estimates of hepatomegaly. Antipyrine clearance had a three-fold range in normal subjects. Its mean value was significantly reduced in each subgroup of patients with liver disease. However, 48% of patients with liver disease had values within the normal range. In normal subjects there was a significant correlation between antipyrine clearance and liver volume. Thus, intersubject variation in clearance normalised for liver volume was less than clearance alone. Antipyrine clearance normalised for liver volume in patients with liver disease was significantly lower than in normal subjects and there was no overlap with normal subjects. In conclusion, assessment of drug metabolising efficiency per unit volume of liver increased the discrimination in differentiating subjects with normal from abnormal livers."}
{"id": "PMID:488758", "title": "Incidence of Crohn's disease in Cardiff between 1934-1977.", "content": "The incidence of Crohn's disease in Cardiff between 1934 and 1977 has been examined using hospital diagnostic indices and supplemented in recent years by personal records from clinicians. A total of 232 cases of Crohn's disease were confirmed after all the notes had been reviewed. There has been a large increase from 0.18/cases/10(5) of the population per year in the 1930s to 4.8 cases/10(5)/year in the 1970s. The major change in incidence is thought to be real rather than apparent and involves all forms of the disease in each age group. The recognition of Crohn's disease of the colon in recent years appears to have played a minor part in the rise in incidence.", "contents": "Incidence of Crohn's disease in Cardiff between 1934-1977. The incidence of Crohn's disease in Cardiff between 1934 and 1977 has been examined using hospital diagnostic indices and supplemented in recent years by personal records from clinicians. A total of 232 cases of Crohn's disease were confirmed after all the notes had been reviewed. There has been a large increase from 0.18/cases/10(5) of the population per year in the 1930s to 4.8 cases/10(5)/year in the 1970s. The major change in incidence is thought to be real rather than apparent and involves all forms of the disease in each age group. The recognition of Crohn's disease of the colon in recent years appears to have played a minor part in the rise in incidence."}
{"id": "PMID:488759", "title": "Effect of oral 1,25 dihydroxycholecalciferol on calcium and phosphate malabsorption in primary biliary cirrhosis.", "content": "Changes in calcium and phosphate absorption in response to treatment with small doses of oral 1,25 (OH)2D3 were studied in 10 patients with primary biliary cirrhosis by means of a combined radio-isotope technique. There was a marked improvement in the fractional rates of absorption of calcium (P less than 0.01) and phosphate (0.05 P less than 0.1) after treatment. This implies than there is no end organ unresponsiveness to the action of active Vitamin D metabolites at the intestinal level in primary biliary cirrhosis.", "contents": "Effect of oral 1,25 dihydroxycholecalciferol on calcium and phosphate malabsorption in primary biliary cirrhosis. Changes in calcium and phosphate absorption in response to treatment with small doses of oral 1,25 (OH)2D3 were studied in 10 patients with primary biliary cirrhosis by means of a combined radio-isotope technique. There was a marked improvement in the fractional rates of absorption of calcium (P less than 0.01) and phosphate (0.05 P less than 0.1) after treatment. This implies than there is no end organ unresponsiveness to the action of active Vitamin D metabolites at the intestinal level in primary biliary cirrhosis."}
{"id": "PMID:488762", "title": "Collagenisation of the Disse space in alcoholic liver disease.", "content": "Collagenisation of the space of Disse was systematically assessed to determine its relationship to the clinical and histological manifestations of chronic alcoholic liver disease. Ninety-four chronic alcoholics who had been submitted to biopsy were assessed by clinical manifestations of hepatic dysfunction and by a 17-parameter Combined Clinical and Laboratory Index (CCLI). Liver biopsies were scored for light (LM) and electron-microscopy (EM) abnormalities using a universal scoring system for both. Thirty-five patients with normal liver histology (LM) had an average collagen score of 0.6 +/- 0.1. Twelve cirrhotic patients and 29 with fatty liver, both groups with mild clinical manifestations, did not differ significantly. In 18 cirrhotic patients and five with fatty liver, both groups having severe clinical manifestations, the mean scores were 2.1 +/- 0.8 (P less than 0.02) and 2.5 +/- 0.6 (P less than 0.01) respectively. Collagenisation also correlated with CCLI (P less than 0.001), serum bilirubin (P less than 0.001), serum aspartate transferase (SGOT) (P less than 0.003), and clinical evidence of portal hypertension and histological changes of necrosis, inflammation, and terminal hepatic vein sclerosis. These results suggest that collagenisation of the Disse space may be important in the pathogenesis of alcoholic liver disease.", "contents": "Collagenisation of the Disse space in alcoholic liver disease. Collagenisation of the space of Disse was systematically assessed to determine its relationship to the clinical and histological manifestations of chronic alcoholic liver disease. Ninety-four chronic alcoholics who had been submitted to biopsy were assessed by clinical manifestations of hepatic dysfunction and by a 17-parameter Combined Clinical and Laboratory Index (CCLI). Liver biopsies were scored for light (LM) and electron-microscopy (EM) abnormalities using a universal scoring system for both. Thirty-five patients with normal liver histology (LM) had an average collagen score of 0.6 +/- 0.1. Twelve cirrhotic patients and 29 with fatty liver, both groups with mild clinical manifestations, did not differ significantly. In 18 cirrhotic patients and five with fatty liver, both groups having severe clinical manifestations, the mean scores were 2.1 +/- 0.8 (P less than 0.02) and 2.5 +/- 0.6 (P less than 0.01) respectively. Collagenisation also correlated with CCLI (P less than 0.001), serum bilirubin (P less than 0.001), serum aspartate transferase (SGOT) (P less than 0.003), and clinical evidence of portal hypertension and histological changes of necrosis, inflammation, and terminal hepatic vein sclerosis. These results suggest that collagenisation of the Disse space may be important in the pathogenesis of alcoholic liver disease."}
{"id": "PMID:488763", "title": "Faecal bile acid loss and bile acid pool size during short-term treatment with ursodeoxycholic and chenodeoxycholic acid in patients with radiolucent gallstones.", "content": "Twelve non-obese patients with radiolucent gallstones were fed on a standard diet. After 10 days (period A), six patients received 15 mg/kg/day of ursodeoxycholic acid (UDCA) (group I) and the other six (group II) the same dose of chenodeoxycholic acid (CDCA) for 15 days (period B). An intravenous injection of 20 micro Ci of 14C-UDCA and of 14C-CDCA was given on the 11th day of period B to the patients of group I and II respectively. Stools were collected at the end of period A and B and one bile sample was collected on the 12th day of period B. The faecal bile acid loss was higher during chenotherapy (36.12 mumol/kg/day) than during ursotherapy (23.94 mumol/kg/day), as was the proportion of lithocholic acid (73% vs 43%) in the faeces. Decay constant rate of faecal radioactivity was 0.365 day-1 in group I and 0.642 in group II. The results indicate that faecal bile acid excretion and turnover rate are greater during CDCA than UDCA, while UDCA increases the bile acid pool size to an even greater extent than does CDCA (150.2 vs 94.9 mumol/kg). This is probably because the former is more slowly degraded to poorly reabsorbable compounds. In fact, the bile saturation index was 0.66 in group I and 1.05 in group II, even though biliary CDCA in the latter had risen to 69.6%.", "contents": "Faecal bile acid loss and bile acid pool size during short-term treatment with ursodeoxycholic and chenodeoxycholic acid in patients with radiolucent gallstones. Twelve non-obese patients with radiolucent gallstones were fed on a standard diet. After 10 days (period A), six patients received 15 mg/kg/day of ursodeoxycholic acid (UDCA) (group I) and the other six (group II) the same dose of chenodeoxycholic acid (CDCA) for 15 days (period B). An intravenous injection of 20 micro Ci of 14C-UDCA and of 14C-CDCA was given on the 11th day of period B to the patients of group I and II respectively. Stools were collected at the end of period A and B and one bile sample was collected on the 12th day of period B. The faecal bile acid loss was higher during chenotherapy (36.12 mumol/kg/day) than during ursotherapy (23.94 mumol/kg/day), as was the proportion of lithocholic acid (73% vs 43%) in the faeces. Decay constant rate of faecal radioactivity was 0.365 day-1 in group I and 0.642 in group II. The results indicate that faecal bile acid excretion and turnover rate are greater during CDCA than UDCA, while UDCA increases the bile acid pool size to an even greater extent than does CDCA (150.2 vs 94.9 mumol/kg). This is probably because the former is more slowly degraded to poorly reabsorbable compounds. In fact, the bile saturation index was 0.66 in group I and 1.05 in group II, even though biliary CDCA in the latter had risen to 69.6%."}
{"id": "PMID:488764", "title": "Reappraisal of the secretory potency and disappearance rate of pure human minigastrin.", "content": "The secretory potency and disappearance rates of pure synthetic human non-sulphated minigastrin (HG-14-I) and pure natural human non-sulphated heptadecapeptide (HG-17-I) were compared in five dogs with gastric fistulas and Heidenhain pouches. Intravenous infusion of equimolar doses of the two gastrins produced equimolar increases over basal of serum immunoreactive gastrin and no statistically significant differences in acid output. Also HG-14-I and HG-17-I did not differ significantly in half-times for disappearance, clearance rates, calculated volumes of distribution, or mean plateau serum levels.", "contents": "Reappraisal of the secretory potency and disappearance rate of pure human minigastrin. The secretory potency and disappearance rates of pure synthetic human non-sulphated minigastrin (HG-14-I) and pure natural human non-sulphated heptadecapeptide (HG-17-I) were compared in five dogs with gastric fistulas and Heidenhain pouches. Intravenous infusion of equimolar doses of the two gastrins produced equimolar increases over basal of serum immunoreactive gastrin and no statistically significant differences in acid output. Also HG-14-I and HG-17-I did not differ significantly in half-times for disappearance, clearance rates, calculated volumes of distribution, or mean plateau serum levels."}
{"id": "PMID:488765", "title": "Biochemical changes in the jejunal mucosa of dogs with naturally occurring exocrine pancreatic insufficiency.", "content": "The roles of extracellular and intracellular mechanisms in the degradation of brush border proteins have been investigated by studying the small intestinal mucosa of dogs with naturally occurring exocrine pancreatic insufficiency. Peroral jejunal biopsies were homogenised and the organelles separated by isopycnic centrifugation on continuous sucrose density gradients. The distributions of marker enzymes for the principal subcellular organelles were determined in the gradients and related to the specific activities in the homogenates. There were increased activities of the brush border carbohydrases zinc-resistant alpha-glucosidase, maltase and sucrase in the pancreatic insufficient animals, but no change in lactase activity. The activity of gamma-glutamyl transferase was also higher in the affected group; the activities of two other brush border enzymes, alkaline phosphatase and leucyl-beta-naphthylamidase, however, were unaltered. These findings with an increase in the modal density of the brush border from 1.20 to 1.22 are consistent with an enhanced glycoprotein content of the microvillus membrane. There were also rises in the activities of lysosomal enzymes. N-Acetyl-beta-glucosaminidase activity was increased in the soluble fractions and the percentage latent enzyme activity was reduced, findings indicative of an increased fragility of the lysosomal membrane. There were no marked alterations in the activities or density gradient distributions of marker enzymes for the other organelles, stressing the specificity of the changes in the brush borders and lysosomes. These findings are compatible with the degradation of certain exposed brush border proteins by pancreatic proteases and suggest that when this is defective, intracellular degradative mechanisms may be stimulated.", "contents": "Biochemical changes in the jejunal mucosa of dogs with naturally occurring exocrine pancreatic insufficiency. The roles of extracellular and intracellular mechanisms in the degradation of brush border proteins have been investigated by studying the small intestinal mucosa of dogs with naturally occurring exocrine pancreatic insufficiency. Peroral jejunal biopsies were homogenised and the organelles separated by isopycnic centrifugation on continuous sucrose density gradients. The distributions of marker enzymes for the principal subcellular organelles were determined in the gradients and related to the specific activities in the homogenates. There were increased activities of the brush border carbohydrases zinc-resistant alpha-glucosidase, maltase and sucrase in the pancreatic insufficient animals, but no change in lactase activity. The activity of gamma-glutamyl transferase was also higher in the affected group; the activities of two other brush border enzymes, alkaline phosphatase and leucyl-beta-naphthylamidase, however, were unaltered. These findings with an increase in the modal density of the brush border from 1.20 to 1.22 are consistent with an enhanced glycoprotein content of the microvillus membrane. There were also rises in the activities of lysosomal enzymes. N-Acetyl-beta-glucosaminidase activity was increased in the soluble fractions and the percentage latent enzyme activity was reduced, findings indicative of an increased fragility of the lysosomal membrane. There were no marked alterations in the activities or density gradient distributions of marker enzymes for the other organelles, stressing the specificity of the changes in the brush borders and lysosomes. These findings are compatible with the degradation of certain exposed brush border proteins by pancreatic proteases and suggest that when this is defective, intracellular degradative mechanisms may be stimulated."}
{"id": "PMID:488766", "title": "Intestinal permeability in rats infected by Nippostrongylus brasiliensis.", "content": "Passive intestinal permeability has been investigated in rats infected by the nematode, Nippostrongylus brasiliensis, by the simultaneous administration of two probe molecules. Experiments using a closed intestinal loop show that there is a significantly increased absorption of lactulose and decreased absorption of mannitol in rats at the 10th to 11th day of infection. Experiments using serial oral administration techniques show that these changes start during the second week of the infection with a return towards normal values by the end of the third week. The results are similar to those found in human coeliac disease and add weight to previous studies which have stressed the similarity in the two disease processes.", "contents": "Intestinal permeability in rats infected by Nippostrongylus brasiliensis. Passive intestinal permeability has been investigated in rats infected by the nematode, Nippostrongylus brasiliensis, by the simultaneous administration of two probe molecules. Experiments using a closed intestinal loop show that there is a significantly increased absorption of lactulose and decreased absorption of mannitol in rats at the 10th to 11th day of infection. Experiments using serial oral administration techniques show that these changes start during the second week of the infection with a return towards normal values by the end of the third week. The results are similar to those found in human coeliac disease and add weight to previous studies which have stressed the similarity in the two disease processes."}
{"id": "PMID:488767", "title": "Water-holding by dietary fibre in vitro and its relationship to faecal output in man.", "content": "The in vitro water-holding properties of 17 dietary fibre preparations, mainly food materials, bulk laxatives, and gel-forming polysaccharides, have been measured. Water uptake was measured by a centrifugation technique and also by a new method using sacs of dialysis tubing containing the material, immersed in simulated gut contents. The centrifugation technique could not be applied to gel-forming polysaccharides but the methods gave broadly similar results for other materials (r=0.85). The gel-forming polysaccharides in general held more water than the food fibres. Studies of matched pairs of materials which differed only slightly in chemical composition suggested that the presence of charged groups on the molecule encouraged water uptake. In the food materials water uptake was related to uronic acid content (r=0.87). Materials ground to a smaller particle size increase their water holding but this effect was small (+28%). The hypothesis that dietary fibre increases faecal bulk by virtue of its ability to hold water was tested by comparing the in vitro water-holding capacity of eight of the fibres with the changes they had produced when fed to human volunteers under controlled conditions. Of these materials pectin had the greatest water-holding capacity (56.2 g water/g material) but produced the smallest change in faecal weight (19%), while bran had the lowest water-holding (4.2 g/g) and the largest faecal weight changes (117%). Overall an inverse relationship (r=0.88) between water-holding and faecal bulking was found, suggesting that dietary fibre does not exert its effect on faecal weight simply by retaining water in the gut.", "contents": "Water-holding by dietary fibre in vitro and its relationship to faecal output in man. The in vitro water-holding properties of 17 dietary fibre preparations, mainly food materials, bulk laxatives, and gel-forming polysaccharides, have been measured. Water uptake was measured by a centrifugation technique and also by a new method using sacs of dialysis tubing containing the material, immersed in simulated gut contents. The centrifugation technique could not be applied to gel-forming polysaccharides but the methods gave broadly similar results for other materials (r=0.85). The gel-forming polysaccharides in general held more water than the food fibres. Studies of matched pairs of materials which differed only slightly in chemical composition suggested that the presence of charged groups on the molecule encouraged water uptake. In the food materials water uptake was related to uronic acid content (r=0.87). Materials ground to a smaller particle size increase their water holding but this effect was small (+28%). The hypothesis that dietary fibre increases faecal bulk by virtue of its ability to hold water was tested by comparing the in vitro water-holding capacity of eight of the fibres with the changes they had produced when fed to human volunteers under controlled conditions. Of these materials pectin had the greatest water-holding capacity (56.2 g water/g material) but produced the smallest change in faecal weight (19%), while bran had the lowest water-holding (4.2 g/g) and the largest faecal weight changes (117%). Overall an inverse relationship (r=0.88) between water-holding and faecal bulking was found, suggesting that dietary fibre does not exert its effect on faecal weight simply by retaining water in the gut."}
{"id": "PMID:488768", "title": "Relationship between amylase concentration, L/S ratio and lecithin concentrations in amniotic fluid.", "content": "Amylase concentration, L/S ratio and lecithin concentration were measured in 110 samples of amniotic fluid in 106 uneventful pregnancies. Amylase concentrations lower than 200 U/l corresponded significantly (p less than 0.001) to a gestational age less than the 37th week as well as to a L/S ratio lower than 2 and to lecithin concentrations lower than 3.5 mg%. Furthermore, amylase values higher than 300 U/l corresponded significantly (p less than 0.001) to a gestational age over the 37th week as to an L/S ratio over 2 and to lecithin concentrations over 3.5 mg%. On the other hand amylase concentrations ranging between 200 and 300 U/l failed to show any significant relationship neither to the age of gestation nor to the concentration of phospholipids. These findings show that the amylase test is a useful method for prediction of fetal maturity. Since the procedure of determination of the amylase concentration is very simple, cheap and rapid, the test can be used as screening method. However, when amylase concentrations are found to range between 200 and 300 U/l specific tests for assessment of fetal maturity have to be applied.", "contents": "Relationship between amylase concentration, L/S ratio and lecithin concentrations in amniotic fluid. Amylase concentration, L/S ratio and lecithin concentration were measured in 110 samples of amniotic fluid in 106 uneventful pregnancies. Amylase concentrations lower than 200 U/l corresponded significantly (p less than 0.001) to a gestational age less than the 37th week as well as to a L/S ratio lower than 2 and to lecithin concentrations lower than 3.5 mg%. Furthermore, amylase values higher than 300 U/l corresponded significantly (p less than 0.001) to a gestational age over the 37th week as to an L/S ratio over 2 and to lecithin concentrations over 3.5 mg%. On the other hand amylase concentrations ranging between 200 and 300 U/l failed to show any significant relationship neither to the age of gestation nor to the concentration of phospholipids. These findings show that the amylase test is a useful method for prediction of fetal maturity. Since the procedure of determination of the amylase concentration is very simple, cheap and rapid, the test can be used as screening method. However, when amylase concentrations are found to range between 200 and 300 U/l specific tests for assessment of fetal maturity have to be applied."}
{"id": "PMID:488769", "title": "Maternal hypotension: fetal outcome in treated and untreated cases.", "content": "Within the last 3 years a prospective study on 70 pregnant women suffering from hypotension was carried out. In all these patients the endocrine placental function was examined regularly by measuring the HPL and E3 level in the maternal serum (from the 16th week of pregnancy onwards). In order to get information on the uteroplacental perfusion rate, placental flow measurements using radioisotopes were carried out. Whereas the endocrine parameters revealed no abnormality, the results of the flow measurements were significantly low in more than 80%. In 30 out of the total of 70 patients no medication was given and after termination of pregnancy the fetal outcome was investigated. The neonates of this group were significantly smaller than normal, the rate of dystrophy was considerably high. The other 40 women were subjected to a drug regimen. Depending on pressure values and type of complaints they were given a crystal suspension of deoxycorticosterone trimethylacetate intramuscularly. In this group of patients the placental perfusion rate improved significantly after medication, the fetal outcome did not differ in comparison to cases with normotension. In the light of our study we suggest that a maternal blood pressure of 110/65 and below has to be treated not only because of maternal indication, but as well because of the fetus' sake.", "contents": "Maternal hypotension: fetal outcome in treated and untreated cases. Within the last 3 years a prospective study on 70 pregnant women suffering from hypotension was carried out. In all these patients the endocrine placental function was examined regularly by measuring the HPL and E3 level in the maternal serum (from the 16th week of pregnancy onwards). In order to get information on the uteroplacental perfusion rate, placental flow measurements using radioisotopes were carried out. Whereas the endocrine parameters revealed no abnormality, the results of the flow measurements were significantly low in more than 80%. In 30 out of the total of 70 patients no medication was given and after termination of pregnancy the fetal outcome was investigated. The neonates of this group were significantly smaller than normal, the rate of dystrophy was considerably high. The other 40 women were subjected to a drug regimen. Depending on pressure values and type of complaints they were given a crystal suspension of deoxycorticosterone trimethylacetate intramuscularly. In this group of patients the placental perfusion rate improved significantly after medication, the fetal outcome did not differ in comparison to cases with normotension. In the light of our study we suggest that a maternal blood pressure of 110/65 and below has to be treated not only because of maternal indication, but as well because of the fetus' sake."}
{"id": "PMID:488770", "title": "Osteoid osteoma of the capitate bone.", "content": "A case of osteoid osteoma of the capitate bone is presented. The osteoma was accompanied by synovial changes in the neighbouring intercarpal joint, and this characterized the clinical features. Resection of the osteoma afforded relief from symptoms.", "contents": "Osteoid osteoma of the capitate bone. A case of osteoid osteoma of the capitate bone is presented. The osteoma was accompanied by synovial changes in the neighbouring intercarpal joint, and this characterized the clinical features. Resection of the osteoma afforded relief from symptoms."}
{"id": "PMID:488771", "title": "The termination of flexor tendon sheaths.", "content": "Fifty-five preserved cadaver digits were injected to show the termination of the flexor tendon sheath. The angle between the termination of the sheath and the most proximal dorsal and ventral bony points of the distal phalanx was measured. Using this concept of angles, a clinical guide to the region containing the sheath is given.", "contents": "The termination of flexor tendon sheaths. Fifty-five preserved cadaver digits were injected to show the termination of the flexor tendon sheath. The angle between the termination of the sheath and the most proximal dorsal and ventral bony points of the distal phalanx was measured. Using this concept of angles, a clinical guide to the region containing the sheath is given."}
{"id": "PMID:488772", "title": "The volar carpal ligament.", "content": "The volar carpal ligament, or superficial part of the flexor retinaculum, is described. A triangular area is identified on the ulnar side of the flexor retinaculum.", "contents": "The volar carpal ligament. The volar carpal ligament, or superficial part of the flexor retinaculum, is described. A triangular area is identified on the ulnar side of the flexor retinaculum."}
{"id": "PMID:488773", "title": "The vascularization of the human flexor pollicis longus tendon.", "content": "The vascularization of the human flexor pollicis longus tendon was outlined by the use of microangiographic techniques, applied on freshly amputated arms. At the insertion of the tendon there was regularly a very well developed vinculum brevis, often extending proximally to the middle of the base phalanx of the thumb. Proximally to the metacarpo phalangeal joint there was a mesotenon of various shape, originating from the volar-ulnar (non-friction) side of the synovial tendon sheath. There seemed to exist a non-interrupted intrinsic longitudinal vascular system in the tendon, and the vessels were located in those parts of the tendon which are not subjected to friction.", "contents": "The vascularization of the human flexor pollicis longus tendon. The vascularization of the human flexor pollicis longus tendon was outlined by the use of microangiographic techniques, applied on freshly amputated arms. At the insertion of the tendon there was regularly a very well developed vinculum brevis, often extending proximally to the middle of the base phalanx of the thumb. Proximally to the metacarpo phalangeal joint there was a mesotenon of various shape, originating from the volar-ulnar (non-friction) side of the synovial tendon sheath. There seemed to exist a non-interrupted intrinsic longitudinal vascular system in the tendon, and the vessels were located in those parts of the tendon which are not subjected to friction."}
{"id": "PMID:488774", "title": "Planning treatment of the rheumatoid hand.", "content": "A detailed assessment of the patient as a whole is an essential pre-requisite to the successful planning and execution of surgical treatment of the rheumatoid hand. In particular the decision whether or not to operate must emerge from a careful weighing of the severity of the patient's symptoms, signs and physical disability against the benefits which are likely to accrue from surgical treatment assessed in relation to the patient's own individual requirements. The author presents his personal reflections on the relative merits of the commonly used surgical procedures with reference to their ability to relieve pain, restore function, achieve cosmetic improvement, and prevent further damage, while at the same time carrying minimal risk of further damage to the already impaired function of the hand. Finally, the philosophy which should govern the surgeon - patient relationship during the management of a chronic progressive disease is examined. That it is absolutely essential to establish a sound rapport from early in the surgical programme is strongly emphasised. Some guidelines as to the design of the programme are also provided.", "contents": "Planning treatment of the rheumatoid hand. A detailed assessment of the patient as a whole is an essential pre-requisite to the successful planning and execution of surgical treatment of the rheumatoid hand. In particular the decision whether or not to operate must emerge from a careful weighing of the severity of the patient's symptoms, signs and physical disability against the benefits which are likely to accrue from surgical treatment assessed in relation to the patient's own individual requirements. The author presents his personal reflections on the relative merits of the commonly used surgical procedures with reference to their ability to relieve pain, restore function, achieve cosmetic improvement, and prevent further damage, while at the same time carrying minimal risk of further damage to the already impaired function of the hand. Finally, the philosophy which should govern the surgeon - patient relationship during the management of a chronic progressive disease is examined. That it is absolutely essential to establish a sound rapport from early in the surgical programme is strongly emphasised. Some guidelines as to the design of the programme are also provided."}
{"id": "PMID:488775", "title": "Abnormal dermatoglyphics in arthrogryposis multiplex congenita.", "content": "Dermatoglyphic abnormalities have been found in patients with arthrogryposis multiple congenita. These unusual features, which appear to be pathognomonic, indicate an early prenatal pathogenesis and may aid in differential diagnosis.", "contents": "Abnormal dermatoglyphics in arthrogryposis multiplex congenita. Dermatoglyphic abnormalities have been found in patients with arthrogryposis multiple congenita. These unusual features, which appear to be pathognomonic, indicate an early prenatal pathogenesis and may aid in differential diagnosis."}
{"id": "PMID:488776", "title": "Trapezio-metacarpal arthroplasty by total prosthesis.", "content": "Thirty-four total trapezio-metacarpal prostheses have been fitted since 1971. Twenty-eight have had a follow-up for more than six months (up to a maximum of five years) and allow a valid assessment of the situation. The operations were undertaken for rheumatoid, osteoarthritic disease, and following trauma. The reasons were either pain, mechanical instability, or stiffness. Two out of three times results were good. The best results were obtained for pain and stability. Those where the indication was stiffness were less satisfying. There were five cases of loosening of the trapezial cup due to operative errors. The mediocre results in general are caused by incomplete treatment allowing an associated lesion to develop. Greater strictness in the indications and in carrying out of operation ought to ensure better results.", "contents": "Trapezio-metacarpal arthroplasty by total prosthesis. Thirty-four total trapezio-metacarpal prostheses have been fitted since 1971. Twenty-eight have had a follow-up for more than six months (up to a maximum of five years) and allow a valid assessment of the situation. The operations were undertaken for rheumatoid, osteoarthritic disease, and following trauma. The reasons were either pain, mechanical instability, or stiffness. Two out of three times results were good. The best results were obtained for pain and stability. Those where the indication was stiffness were less satisfying. There were five cases of loosening of the trapezial cup due to operative errors. The mediocre results in general are caused by incomplete treatment allowing an associated lesion to develop. Greater strictness in the indications and in carrying out of operation ought to ensure better results."}
{"id": "PMID:488778", "title": "The mechanical stability of internal fixation of fractured phalanges.", "content": "The rigidity of five techniques of internal fixation of fractured phalanges is tested experimentally and compared. The clinical relevance is discussed.", "contents": "The mechanical stability of internal fixation of fractured phalanges. The rigidity of five techniques of internal fixation of fractured phalanges is tested experimentally and compared. The clinical relevance is discussed."}
{"id": "PMID:488779", "title": "The neuro vascular flap in finger tip injuries.", "content": "The Kutler technique to restore skin cover in finger tip injuries was modified by mobilising the lateral triangular flap completely on its neuro-vascular bundle. Resultant increased mobility permitted greater advancement of the flap to allow adequate cover of the denuded finger tip. A unilateral flap is often sufficient.", "contents": "The neuro vascular flap in finger tip injuries. The Kutler technique to restore skin cover in finger tip injuries was modified by mobilising the lateral triangular flap completely on its neuro-vascular bundle. Resultant increased mobility permitted greater advancement of the flap to allow adequate cover of the denuded finger tip. A unilateral flap is often sufficient."}
{"id": "PMID:488780", "title": "Youngest replantation with microsurgical anastomoses.", "content": "A successful replantation of a finger on an infant aged twelve months and fifteen days by microsurgical repair is reported.", "contents": "Youngest replantation with microsurgical anastomoses. A successful replantation of a finger on an infant aged twelve months and fifteen days by microsurgical repair is reported."}
{"id": "PMID:488782", "title": "Rupture of the extensor pollicis longus tendon after fracture of the lower end of the radius--a clinical and microangiographic study.", "content": "The pathogenesis of the late post-traumatic rupture of the extensor pollicis longus tendon has never been satisfactorily explained. In the present series of fifty-nine ruptures two were partial, making possible an exact localization of the rupture. Microangiographic studies performed on amputated arms showed that this part of the tendon was poorly vascularized. Our study confirms earlier observations that ruptures most commonly occur after undisplaced fractures. It is suggested that increased pressure within the non-ruptured tendon sheath jeopardizes the blood flow in the already poorly vascularized parts of the tendon, leading to degeneration and rupture, usually within eight weeks. An haematoma inside the sheath interfering with the production of synovial fluid, could deprive the tendon of an alternative nutrition via diffusional pathways.", "contents": "Rupture of the extensor pollicis longus tendon after fracture of the lower end of the radius--a clinical and microangiographic study. The pathogenesis of the late post-traumatic rupture of the extensor pollicis longus tendon has never been satisfactorily explained. In the present series of fifty-nine ruptures two were partial, making possible an exact localization of the rupture. Microangiographic studies performed on amputated arms showed that this part of the tendon was poorly vascularized. Our study confirms earlier observations that ruptures most commonly occur after undisplaced fractures. It is suggested that increased pressure within the non-ruptured tendon sheath jeopardizes the blood flow in the already poorly vascularized parts of the tendon, leading to degeneration and rupture, usually within eight weeks. An haematoma inside the sheath interfering with the production of synovial fluid, could deprive the tendon of an alternative nutrition via diffusional pathways."}
{"id": "PMID:488783", "title": "Surgical repair of the boutonni\u00e8re deformity of the fingers.", "content": "Boutonni\u00e8re is discussed. The results of twenty-seven digits operated on are presented. The operative technique used was that directed to the central tendon. The results were analysed according to the digit affected, to the type of lesion, if closed or opened, to the association with other lesions and to the time between the lesion and the surgery.", "contents": "Surgical repair of the boutonni\u00e8re deformity of the fingers. Boutonni\u00e8re is discussed. The results of twenty-seven digits operated on are presented. The operative technique used was that directed to the central tendon. The results were analysed according to the digit affected, to the type of lesion, if closed or opened, to the association with other lesions and to the time between the lesion and the surgery."}
{"id": "PMID:488784", "title": "Triggering due to de Quervain's disease.", "content": "Triggering of the thumb due to de Quervain's disease is a rarity. When it occurs in a boy of seven years old, it has never been reported before in the English literature.", "contents": "Triggering due to de Quervain's disease. Triggering of the thumb due to de Quervain's disease is a rarity. When it occurs in a boy of seven years old, it has never been reported before in the English literature."}
{"id": "PMID:488785", "title": "Bilateral ulnar \"thumbs\".", "content": "Congenital fusion of the carpal bones is fairly common and may be associated with other congenital deformities in the hand. This report deals with an unusual congenital anomaly of the little fingers associated with fusion of the triquetrum and lunate. Both little fingers were adducted and rotated giving crude resemblance to thumbs on the ulnar borders of the hands. The deformity was easily corrected by osteotomy through the base of the fifth metacarpals.", "contents": "Bilateral ulnar \"thumbs\". Congenital fusion of the carpal bones is fairly common and may be associated with other congenital deformities in the hand. This report deals with an unusual congenital anomaly of the little fingers associated with fusion of the triquetrum and lunate. Both little fingers were adducted and rotated giving crude resemblance to thumbs on the ulnar borders of the hands. The deformity was easily corrected by osteotomy through the base of the fifth metacarpals."}
{"id": "PMID:488787", "title": "Fractures of the shafts of the phalanges of the hand.", "content": "Fractures of the shafts of the phalanges are common and often disabling. Little detailed information is available as to the results of treatment, against which new methods of treatment may be compared. Thirty-nine comminuted fractures and 109 which were not comminuted were followed-up and their results are presented in detail. In both groups only about 57% obtained satisfactory results. Possible methods of improving these are discussed and the importance of accurate reduction of non comminuted fractures is emphasised.", "contents": "Fractures of the shafts of the phalanges of the hand. Fractures of the shafts of the phalanges are common and often disabling. Little detailed information is available as to the results of treatment, against which new methods of treatment may be compared. Thirty-nine comminuted fractures and 109 which were not comminuted were followed-up and their results are presented in detail. In both groups only about 57% obtained satisfactory results. Possible methods of improving these are discussed and the importance of accurate reduction of non comminuted fractures is emphasised."}
{"id": "PMID:488788", "title": "Fractures of the phalanges of the hand in children.", "content": "Fractures of the phalanges of the hand were studied in 203 children. Fingertip fractures caused disability for three weeks but usually had good results. Comminuted fractures often took the form of longitudinal splitting. Epiphyseal fractures were mostly of the Salter type 2 and metaphyseal types and usually had good results: remodelling occurred in both planes at the base of the proximal phalanx but only in the sagittal plane at the base of the middle phalanx. Epiphyseal fractures at the base of the distal phalanx often had unsatisfactory results. Fractures of the neck of phalanx showed no remodelling at all and if deformity is not corrected it is probably permanent.", "contents": "Fractures of the phalanges of the hand in children. Fractures of the phalanges of the hand were studied in 203 children. Fingertip fractures caused disability for three weeks but usually had good results. Comminuted fractures often took the form of longitudinal splitting. Epiphyseal fractures were mostly of the Salter type 2 and metaphyseal types and usually had good results: remodelling occurred in both planes at the base of the proximal phalanx but only in the sagittal plane at the base of the middle phalanx. Epiphyseal fractures at the base of the distal phalanx often had unsatisfactory results. Fractures of the neck of phalanx showed no remodelling at all and if deformity is not corrected it is probably permanent."}
{"id": "PMID:488789", "title": "Assessment of past results and current practice in the treatment of rheumatoid metacarpophalangeal joints. Five year review.", "content": "A new design of prosthetic finger joint for the rheumatoid patient, and the technique for its insertion is described, which has now been in use for five years. The technique includes the soft tissue correction of the ulnar deviation. Results are reported for all the cases performed in one year giving a two year follow up, assessing flexion strength, pinch strength and the degree of recurrence of ulnar drift associated with loss of pinch strength.", "contents": "Assessment of past results and current practice in the treatment of rheumatoid metacarpophalangeal joints. Five year review. A new design of prosthetic finger joint for the rheumatoid patient, and the technique for its insertion is described, which has now been in use for five years. The technique includes the soft tissue correction of the ulnar deviation. Results are reported for all the cases performed in one year giving a two year follow up, assessing flexion strength, pinch strength and the degree of recurrence of ulnar drift associated with loss of pinch strength."}
{"id": "PMID:488790", "title": "Surgical correction of ulnar drift in the rheumatoid hand.", "content": "A new technique for the correction of ulnar drift is described which has been used by the author in selected cases during the past 5 years. Results of this technique during a 2 year period are compared with those treated by prosthetic joint replacement alone, or a combination of the two methods.", "contents": "Surgical correction of ulnar drift in the rheumatoid hand. A new technique for the correction of ulnar drift is described which has been used by the author in selected cases during the past 5 years. Results of this technique during a 2 year period are compared with those treated by prosthetic joint replacement alone, or a combination of the two methods."}
{"id": "PMID:488791", "title": "Re-alignment procedure for ulnar drift of the metacarpophalangeal joint in rheumatoid arthritis.", "content": "An operation for correcting ulnar deviation in rheumatoid arthritis, by employing local tendon transfers at an early stage in the developing deformity, is described. The pathology, clinical staging and results in 103 joints are presented.", "contents": "Re-alignment procedure for ulnar drift of the metacarpophalangeal joint in rheumatoid arthritis. An operation for correcting ulnar deviation in rheumatoid arthritis, by employing local tendon transfers at an early stage in the developing deformity, is described. The pathology, clinical staging and results in 103 joints are presented."}
{"id": "PMID:488792", "title": "Interpositional arthroplasty of the wrist in rheumatoid arthritis.", "content": "The procedure of interpositional arthroplasty of the wrist joint in rheumatoid arthritis, using silicone sheeting, is described. The results and complications of sixty arthroplasties are presented.", "contents": "Interpositional arthroplasty of the wrist in rheumatoid arthritis. The procedure of interpositional arthroplasty of the wrist joint in rheumatoid arthritis, using silicone sheeting, is described. The results and complications of sixty arthroplasties are presented."}
{"id": "PMID:488793", "title": "The pathogenesis of the rheumatoid swan neck deformity.", "content": "Disappointment with the late results of intrinsic release for the rheumatoid \"intrinsic-plus\" hand has led to a re-appraisal of the role of intrinsic muscle contracture in the pathogenesis of the rheumatoid swan neck deformity. In cadaveric fingers, a properly placed suture typing the lateral band to the middle slip insertion causes a swan neck deformity. It is suggested that the usual \"intrinsic-plus\" hand and the fixed swan neck deformity of rheumatoid arthritis is caused by adhesions between the extensor tendons on the dorsum of the proximal interphalangeal joint, rather than by intrinsic muscle contracture and/or metacarpo-phalangeal dislocation.", "contents": "The pathogenesis of the rheumatoid swan neck deformity. Disappointment with the late results of intrinsic release for the rheumatoid \"intrinsic-plus\" hand has led to a re-appraisal of the role of intrinsic muscle contracture in the pathogenesis of the rheumatoid swan neck deformity. In cadaveric fingers, a properly placed suture typing the lateral band to the middle slip insertion causes a swan neck deformity. It is suggested that the usual \"intrinsic-plus\" hand and the fixed swan neck deformity of rheumatoid arthritis is caused by adhesions between the extensor tendons on the dorsum of the proximal interphalangeal joint, rather than by intrinsic muscle contracture and/or metacarpo-phalangeal dislocation."}
{"id": "PMID:488794", "title": "Perichondrial wrist arthroplasty in rheumatoid patients.", "content": "A new method is proposed for reconstruction of the wrist joint in rheumatoid arthritis. Perichondrial arthroplasty done in four patients with severe deformity of the wrist from rheumatoid arthritis gave functionally improved results. In one case biopsy of the reconstructed joint showed newly formed cartilaginous tissue four months after initial surgery.", "contents": "Perichondrial wrist arthroplasty in rheumatoid patients. A new method is proposed for reconstruction of the wrist joint in rheumatoid arthritis. Perichondrial arthroplasty done in four patients with severe deformity of the wrist from rheumatoid arthritis gave functionally improved results. In one case biopsy of the reconstructed joint showed newly formed cartilaginous tissue four months after initial surgery."}
{"id": "PMID:488795", "title": "The palmar abduction-pronation osteotomy of the first metacarpal bone combined with tendon transfer for lateral thenar muscle paralysis.", "content": "The authors describe a palmar abduction-pronation osteotomy of the first metacarpal bone combined with a tendon transfer for treatment of palsy of the lateral thenar muscles. The results of fourteen cases are reported.", "contents": "The palmar abduction-pronation osteotomy of the first metacarpal bone combined with tendon transfer for lateral thenar muscle paralysis. The authors describe a palmar abduction-pronation osteotomy of the first metacarpal bone combined with a tendon transfer for treatment of palsy of the lateral thenar muscles. The results of fourteen cases are reported."}
{"id": "PMID:488796", "title": "Experience with a two-staged pedicled flexor tendon graft.", "content": "A two-staged technique for flexor tendon grafting in Zone 2 is described using a silicone rubber rod which is replaced at the second stage by a somersaulted superficialis tendon graft sutured end-to-end to profundus tendon at the lumbrical level. The results are analysed.", "contents": "Experience with a two-staged pedicled flexor tendon graft. A two-staged technique for flexor tendon grafting in Zone 2 is described using a silicone rubber rod which is replaced at the second stage by a somersaulted superficialis tendon graft sutured end-to-end to profundus tendon at the lumbrical level. The results are analysed."}
{"id": "PMID:488797", "title": "Treatment of carpal tunnel syndrome. Comparative study with and without epineurolysis.", "content": "Three series of cases of Carpal Tunnel Syndrome have been studied. One series was treated a different, respected surgical team, the second series by the author by decompression alone, and the third series by additional intraneural decompression using the microscope. The authors found very little difference in the results, apart from marginally better results in the presence of thenar muscle involvement.", "contents": "Treatment of carpal tunnel syndrome. Comparative study with and without epineurolysis. Three series of cases of Carpal Tunnel Syndrome have been studied. One series was treated a different, respected surgical team, the second series by the author by decompression alone, and the third series by additional intraneural decompression using the microscope. The authors found very little difference in the results, apart from marginally better results in the presence of thenar muscle involvement."}
{"id": "PMID:488799", "title": "Transverse volar skin laceration of the finger: Sign of volar plate injury.", "content": "Transverse tears of the volar skin of a finger with a hyper-extension injury is associated with frequent volar plate tears or avulsions. Recognition is essential for surgical exploration of the volar plate and supporting joint structures and, if injured, surgical repair should be carried out at the time of skin suturing.", "contents": "Transverse volar skin laceration of the finger: Sign of volar plate injury. Transverse tears of the volar skin of a finger with a hyper-extension injury is associated with frequent volar plate tears or avulsions. Recognition is essential for surgical exploration of the volar plate and supporting joint structures and, if injured, surgical repair should be carried out at the time of skin suturing."}
{"id": "PMID:488800", "title": "Extensor digitorum brevis manus: a case report review.", "content": "A detailed description of bilateral extensor digitorum brevis manus (EDBM) present in a female cadaver is presented. The need for clinical recognition is emphasized. Prevalence, morphological variability and theories of ontogeny are reviewed. Clinical significance and possible function are discussed. Since misdiagnosis may result from the anatomical variability and lack of clinical awareness, the need for further study is emphasized.", "contents": "Extensor digitorum brevis manus: a case report review. A detailed description of bilateral extensor digitorum brevis manus (EDBM) present in a female cadaver is presented. The need for clinical recognition is emphasized. Prevalence, morphological variability and theories of ontogeny are reviewed. Clinical significance and possible function are discussed. Since misdiagnosis may result from the anatomical variability and lack of clinical awareness, the need for further study is emphasized."}
{"id": "PMID:488832", "title": "Procedure for evaluating clinical practice.", "content": "A step-by-step procedure is needed for evaluating social work practice. This article describes such a procedure, with particular emphasis placed on practical concerns that arise in the use of evaluation techniques, and shows that clinical evaluation is compatible and consistent with most problem-solving models already in use by experienced practitioners.", "contents": "Procedure for evaluating clinical practice. A step-by-step procedure is needed for evaluating social work practice. This article describes such a procedure, with particular emphasis placed on practical concerns that arise in the use of evaluation techniques, and shows that clinical evaluation is compatible and consistent with most problem-solving models already in use by experienced practitioners."}
{"id": "PMID:488836", "title": "Herbal medicine in the Puerto Rican community.", "content": "This article focuses on one aspect of health in the Puerto Rican community in the United States--the continuing utilization of herbal medicine and folk healers in the prevention and cure of illness. In addition to providing a fundamental explanation of this system of healing, the author makes recommendations for increasing the satisfaction of Puerto Rican patients with health care services.", "contents": "Herbal medicine in the Puerto Rican community. This article focuses on one aspect of health in the Puerto Rican community in the United States--the continuing utilization of herbal medicine and folk healers in the prevention and cure of illness. In addition to providing a fundamental explanation of this system of healing, the author makes recommendations for increasing the satisfaction of Puerto Rican patients with health care services."}
{"id": "PMID:488837", "title": "Sheltered-care needs of the mentally ill.", "content": "The recent emphasis on housing formerly hospitalized mental patients in community-based facilities has caused the number of such facilities to proliferate. This article examines five characteristics that affect individuals' needs for sheltered care and must be considered in selecting the most appropriate type of facility.", "contents": "Sheltered-care needs of the mentally ill. The recent emphasis on housing formerly hospitalized mental patients in community-based facilities has caused the number of such facilities to proliferate. This article examines five characteristics that affect individuals' needs for sheltered care and must be considered in selecting the most appropriate type of facility."}
{"id": "PMID:488838", "title": "In sickness and in health: future social work roles.", "content": "Increasing specialization, rising costs, and the depersonalization of care are some of the problems affecting the delivery of health care in this country. This article discusses the impact of these and other major developments and proposes expanded tasks and functions to be performed by social workers in the future.", "contents": "In sickness and in health: future social work roles. Increasing specialization, rising costs, and the depersonalization of care are some of the problems affecting the delivery of health care in this country. This article discusses the impact of these and other major developments and proposes expanded tasks and functions to be performed by social workers in the future."}
{"id": "PMID:488839", "title": "Interdisciplinary education for health care professionals.", "content": "As the interdisciplinary approach to health care becomes more important, new approaches to interdisciplinary education for health professionals are needed. This article presents objectives for interdisciplinary learning and describes one such course designed to deal with the problem of child abuse.", "contents": "Interdisciplinary education for health care professionals. As the interdisciplinary approach to health care becomes more important, new approaches to interdisciplinary education for health professionals are needed. This article presents objectives for interdisciplinary learning and describes one such course designed to deal with the problem of child abuse."}
{"id": "PMID:488840", "title": "Collaboration between schools of social work and university medical centers.", "content": "Although the interface involving social work, medicine, and the other health professions occurs primarily in the day-to-day world of practice in hospitals and other health agencies, an equally important opportunity exists for interaction at the university level between schools of social work and schools for health professionals. This artice analyzes one school's effort to build effective interdisciplinary linkages.", "contents": "Collaboration between schools of social work and university medical centers. Although the interface involving social work, medicine, and the other health professions occurs primarily in the day-to-day world of practice in hospitals and other health agencies, an equally important opportunity exists for interaction at the university level between schools of social work and schools for health professionals. This artice analyzes one school's effort to build effective interdisciplinary linkages."}
{"id": "PMID:488841", "title": "Abortion counseling in a general hospital.", "content": "Given the increase in the number of abortions being performed in hospitals throughout the United States and Canada, there is an obvious need for counseling programs for these patients. The authors describe one such program, and emphasize the importance of close working relationships between the counselors and their supervising staff.", "contents": "Abortion counseling in a general hospital. Given the increase in the number of abortions being performed in hospitals throughout the United States and Canada, there is an obvious need for counseling programs for these patients. The authors describe one such program, and emphasize the importance of close working relationships between the counselors and their supervising staff."}
{"id": "PMID:488842", "title": "Are social workers turning a deaf ear? A study of social services to the deaf.", "content": "This study assessed the quality and quantity of social services received by deaf persons residing in Salt Lake County, Utah. It was found that although a majority of human service agencies in the county have had some contact with deaf clients, only those agencies that have made an effort to develop programs especially for the deaf and employ someone who is skilled in sign language could deliver services effectively to this population.", "contents": "Are social workers turning a deaf ear? A study of social services to the deaf. This study assessed the quality and quantity of social services received by deaf persons residing in Salt Lake County, Utah. It was found that although a majority of human service agencies in the county have had some contact with deaf clients, only those agencies that have made an effort to develop programs especially for the deaf and employ someone who is skilled in sign language could deliver services effectively to this population."}
{"id": "PMID:488843", "title": "Helping to manage the emotional effects of arthritis.", "content": "Arthritis is a chronic disease that has no known cause or cure. The disease is often serious and can cause excruciating pain in the joints and other parts of the body. Some people recognize the physical problems associated with arthritis, but few realize the social and emotional impact it can have on the patient and the family. In this article the author discusses how social workers and other therapists can help arthritic patients cope with the disease and live more productively despite their physical limitations.", "contents": "Helping to manage the emotional effects of arthritis. Arthritis is a chronic disease that has no known cause or cure. The disease is often serious and can cause excruciating pain in the joints and other parts of the body. Some people recognize the physical problems associated with arthritis, but few realize the social and emotional impact it can have on the patient and the family. In this article the author discusses how social workers and other therapists can help arthritic patients cope with the disease and live more productively despite their physical limitations."}
{"id": "PMID:488845", "title": "Trends in services for pregnant adolescents.", "content": "Because the rate of childbirth among adolescent girls in the United States is among the highest in the world, a pressing need exists for services for pregnant adolescents. After summarizing trends and indicating the complex problems in this area, the authors put forward recommendations for continued efforts.", "contents": "Trends in services for pregnant adolescents. Because the rate of childbirth among adolescent girls in the United States is among the highest in the world, a pressing need exists for services for pregnant adolescents. After summarizing trends and indicating the complex problems in this area, the authors put forward recommendations for continued efforts."}
{"id": "PMID:488847", "title": "Group work in a hospital waiting room.", "content": "For students who have determination, field-work placements offer an opportunity not only to learn, but to develop innovative services. The authors give a personal account of how, as students, they initiated a program of group work in a hospital waiting room.", "contents": "Group work in a hospital waiting room. For students who have determination, field-work placements offer an opportunity not only to learn, but to develop innovative services. The authors give a personal account of how, as students, they initiated a program of group work in a hospital waiting room."}
{"id": "PMID:488848", "title": "Mutual-help group for patients: taking steps toward change.", "content": "The growing movements in behalf of consumers' rights and self-help in health care were the impetus for the formation of a hospital group program for patients with chronic obstructive lung disease. The authors use case examples and summaries to describe the steps by which these patients began to offer each other help, which in turn led to both personal and institutional change.", "contents": "Mutual-help group for patients: taking steps toward change. The growing movements in behalf of consumers' rights and self-help in health care were the impetus for the formation of a hospital group program for patients with chronic obstructive lung disease. The authors use case examples and summaries to describe the steps by which these patients began to offer each other help, which in turn led to both personal and institutional change."}
{"id": "PMID:488849", "title": "Social work in the small hospital.", "content": "The social worker who practices in a small rural hospital encounters unique problems and opportunities. The author discusses the advantages of being the only social worker on the staff and emphasizes the importance of establishing informal, cooperative relationships with the medical staff and with people and agencies in the community.", "contents": "Social work in the small hospital. The social worker who practices in a small rural hospital encounters unique problems and opportunities. The author discusses the advantages of being the only social worker on the staff and emphasizes the importance of establishing informal, cooperative relationships with the medical staff and with people and agencies in the community."}
{"id": "PMID:488850", "title": "Parental group therapy in the management of a fatal childhood disease.", "content": "Group work enabled parents of children with Duchenne muscular dystrophy to express fears and concerns, and to increase awareness of their attitudes toward themselves, their disabled child, their normal children, and the world at large. This article discusses how the group offered parents a way of exploring processes within the family and assisting in the management of psychosocial problems created in raising a child with a progressive fatal disease.", "contents": "Parental group therapy in the management of a fatal childhood disease. Group work enabled parents of children with Duchenne muscular dystrophy to express fears and concerns, and to increase awareness of their attitudes toward themselves, their disabled child, their normal children, and the world at large. This article discusses how the group offered parents a way of exploring processes within the family and assisting in the management of psychosocial problems created in raising a child with a progressive fatal disease."}
{"id": "PMID:488851", "title": "Quantitative histochemistry of the sorbitol pathway in glomeruli and small arteries of human diabetic kidney.", "content": "Recent evidence has suggested a role for the polyol pathway in pathogenesis of cell damage in diabetes Glucose may be phosphorylated to glucose-6-phosphate via hexokinase and enter glycolysis or reduced to sorbitol via aldose reductase to enter the polyol pathway. The poorly diffusible sorbitol is converted via sorbitol dehydrogenase to fructose. Hexokinase, aldose reductase and sorbitol dehydrogenase activities were measured in glomeruli (G) and small arteries (SA) taken from normal and diabetic human kidneys, Hexokinase in diabetic G was 1688, which was significantly decreased from normal, 3147 mmoles/kg-1/h-1. Alodse reductase was significantly elevated in diabetic G,56-6, compared to normal G,10-8 mmoles/kg-1/h-1. In contrast, sorbitol dehydrogenase was significantly depressed in diabetic G, 3-7 VERSUs 10-9 mmoles/kg-1/h-1. The enzymatic changes observed in diabetic G would facilitate accumulation of sorbitol and therefore could contribute to the progression of glomerulosclerosis. The activity of hexokinase was also significantly reduced in SA, whereas aldose reductase and sorbitol dehydrogenase were unchanged.", "contents": "Quantitative histochemistry of the sorbitol pathway in glomeruli and small arteries of human diabetic kidney. Recent evidence has suggested a role for the polyol pathway in pathogenesis of cell damage in diabetes Glucose may be phosphorylated to glucose-6-phosphate via hexokinase and enter glycolysis or reduced to sorbitol via aldose reductase to enter the polyol pathway. The poorly diffusible sorbitol is converted via sorbitol dehydrogenase to fructose. Hexokinase, aldose reductase and sorbitol dehydrogenase activities were measured in glomeruli (G) and small arteries (SA) taken from normal and diabetic human kidneys, Hexokinase in diabetic G was 1688, which was significantly decreased from normal, 3147 mmoles/kg-1/h-1. Alodse reductase was significantly elevated in diabetic G,56-6, compared to normal G,10-8 mmoles/kg-1/h-1. In contrast, sorbitol dehydrogenase was significantly depressed in diabetic G, 3-7 VERSUs 10-9 mmoles/kg-1/h-1. The enzymatic changes observed in diabetic G would facilitate accumulation of sorbitol and therefore could contribute to the progression of glomerulosclerosis. The activity of hexokinase was also significantly reduced in SA, whereas aldose reductase and sorbitol dehydrogenase were unchanged."}
{"id": "PMID:488852", "title": "Modification of a quantitative laser-spectrographic method of determination of cations contained in tissue slices.", "content": "A quantitative method of determination of elements in histological slices by means of laser microanalysis has been modified. The modification consists essentially in such methodological approach that allows expressing the results by figures which are directly proportional to the relative content of the element in question. The final result, i.e. the relative quantity of the element to be determined is obtained by subtracting the photometric value yielded by the spectrographic line of the Ag standard from that yielded by the spectrographic line of the element under investigation (J = JX-JAG). To avoid negative figures the Ag standard has to be prepared in such a way, as to yield the lowest possibly photometric values still lying on the straight line of the standard curve.", "contents": "Modification of a quantitative laser-spectrographic method of determination of cations contained in tissue slices. A quantitative method of determination of elements in histological slices by means of laser microanalysis has been modified. The modification consists essentially in such methodological approach that allows expressing the results by figures which are directly proportional to the relative content of the element in question. The final result, i.e. the relative quantity of the element to be determined is obtained by subtracting the photometric value yielded by the spectrographic line of the Ag standard from that yielded by the spectrographic line of the element under investigation (J = JX-JAG). To avoid negative figures the Ag standard has to be prepared in such a way, as to yield the lowest possibly photometric values still lying on the straight line of the standard curve."}
{"id": "PMID:488853", "title": "Comparative histochemical studies of iron absorption in three fresh water teleosts.", "content": "Absorption of iron in the intestine of three different species of fishes, namely; Claris batrachus, Channa striatus and Esomus danricus has been described. These three species of fishes have different feeding habits, being omnivorus, carnivorus and herbivorus respectively. The absorption of iron has been studied at different time intervals; after three hours, six hours and nine hours. The mucosal epithelial cells, submucosal blood vessels and blood capilaries were the main site of iron absorption. A comparative account of absorption of iron of all three fishes is described and discussed.", "contents": "Comparative histochemical studies of iron absorption in three fresh water teleosts. Absorption of iron in the intestine of three different species of fishes, namely; Claris batrachus, Channa striatus and Esomus danricus has been described. These three species of fishes have different feeding habits, being omnivorus, carnivorus and herbivorus respectively. The absorption of iron has been studied at different time intervals; after three hours, six hours and nine hours. The mucosal epithelial cells, submucosal blood vessels and blood capilaries were the main site of iron absorption. A comparative account of absorption of iron of all three fishes is described and discussed."}
{"id": "PMID:488854", "title": "Cytochemical study of acid phosphatase activity in the functional and degenerating spinning gland cells of Pericallia ricinii (lepidoptera).", "content": "A study of the presence, distribution and activity of acid phosphatase has been carried out in the cells of the spinning gland of moth pest Pericallia ricinii in sequential larval, prepupal and pupal stages. Acid phosphomonoesterase activity at first appears in the apical and basal regions of the secretory cells of second instar larva and later in the following stages is localised in the middle region of the cells. The highest activity was found while using an incubation time from 25 to 30 minutes. Prolonged incubation resulted in artifacts. Based on the acid phosphatase activity two cell types (A and B) in the spinning gland cell layer could be distinguished. The cytochemical differences in histologically similar cells of the spinning gland of the Indian moth pest were reported for the first time. In pupal period progressive deformation could be observed in the gland cells, which is accompanied by high acid phosphatase activity, which is in contradistinction to cells with small deformations and low enzyme activity. The process of degeneration in former cells is also quicker than in the latter ones.", "contents": "Cytochemical study of acid phosphatase activity in the functional and degenerating spinning gland cells of Pericallia ricinii (lepidoptera). A study of the presence, distribution and activity of acid phosphatase has been carried out in the cells of the spinning gland of moth pest Pericallia ricinii in sequential larval, prepupal and pupal stages. Acid phosphomonoesterase activity at first appears in the apical and basal regions of the secretory cells of second instar larva and later in the following stages is localised in the middle region of the cells. The highest activity was found while using an incubation time from 25 to 30 minutes. Prolonged incubation resulted in artifacts. Based on the acid phosphatase activity two cell types (A and B) in the spinning gland cell layer could be distinguished. The cytochemical differences in histologically similar cells of the spinning gland of the Indian moth pest were reported for the first time. In pupal period progressive deformation could be observed in the gland cells, which is accompanied by high acid phosphatase activity, which is in contradistinction to cells with small deformations and low enzyme activity. The process of degeneration in former cells is also quicker than in the latter ones."}
{"id": "PMID:488855", "title": "Influence of lead poisoning and ultrastructural changes in the body wall of Eisenia foetida (Savigny), Oligochaeta. I. Short action of different concentrations of lead and ultrastructural changes in the cells of the body wall.", "content": "The effect of short term influence of different concentrations of Pb(NO3)2 in the soil on the accumulation and localization of lead in the body wall cells of the earthworm Eisenia foetida (Savigny), Oligocheta, was studied histochemically. The experimental animals displayed the presence of lead deposits in the cytoplasm and vacuols of the epithelial gland cells which also showed morphological changes indicative of their increased secretory activity. Furthermore, a considerable accumulation of lead was found in the sarcoplasm and particularly in the sarcoplasmic reticulum of the muscle cells in the body wall. The histochemical data show that the most intense accumulation of lead is found in animals kept in an environment containing 0-05 mg Pb per 1 g of the soil. At this concentration of lead noteworthy morphological changes together with deposits of this metal are found in the mitochondria. This mitochondrial changes occurred together with the above mentioned, lead induced cytological deviations. They did not appear in other groups of experimental animals.", "contents": "Influence of lead poisoning and ultrastructural changes in the body wall of Eisenia foetida (Savigny), Oligochaeta. I. Short action of different concentrations of lead and ultrastructural changes in the cells of the body wall. The effect of short term influence of different concentrations of Pb(NO3)2 in the soil on the accumulation and localization of lead in the body wall cells of the earthworm Eisenia foetida (Savigny), Oligocheta, was studied histochemically. The experimental animals displayed the presence of lead deposits in the cytoplasm and vacuols of the epithelial gland cells which also showed morphological changes indicative of their increased secretory activity. Furthermore, a considerable accumulation of lead was found in the sarcoplasm and particularly in the sarcoplasmic reticulum of the muscle cells in the body wall. The histochemical data show that the most intense accumulation of lead is found in animals kept in an environment containing 0-05 mg Pb per 1 g of the soil. At this concentration of lead noteworthy morphological changes together with deposits of this metal are found in the mitochondria. This mitochondrial changes occurred together with the above mentioned, lead induced cytological deviations. They did not appear in other groups of experimental animals."}
{"id": "PMID:488856", "title": "Sex differences in adrenocortical structure and function. V. The effects of postpubertal gonadectomy and gonadal hormone replacement on nuclear-cytoplasmic ratio, morphology and histochemistry of rat adrenal cortex.", "content": "Studies were carried out on adult rats of Wistar strain. Six weeks after postpubertal gonadectomy some of the orchiectomized rats were injected with a single dose of testosterone cypionate and ovari-ctomized rats with estradiol cypionate (17 beta-cyclopentyloproprionate esters of testosterone or estradiol). The control rats were sham operated. Investigations were carried out 8 weeks after surgery. Absolute and relative adrenal weight is lower in the male than in the female rat. Orchiectomy increases these weights while testosterone restores them to their normal level. Ovariectomy has no effect on the weight of adrenal, estradiol, however, increases the relative weight of the gland. The adrenal cortex of the adult female is wider than in the adult male rat and in female gland sudanophobic zone is lacking. Orchiectomy leads to the broadening of the adrenal cortex and testosterone replacement has an opposite effect. Ovariectomy has no effect on the structure of adrenal cortex and estradiol replacement resulted in the narrowing of zona glomerulosa. There were no differences in the nuclear-cytoplasmic ratio of zona glomerulosa cells in male and Female rats and neither gonadectomy nor gonadal hormone replacement has no effect on this parameter. The nuclear-cytoplasmic ratio of zona fasciculata cells in the male is markedly higher than in female. Orchiectomy lowers this ratio and testosterone restores it to the normal level. Neither ovariectomy nor estradiol replacement has effect on the nuclear-cytoplasmic ratio of zona fasciculata cells. Similar changes as those in the zona fasciculata were observed in zona reticularis cells. Among the oxidoreductases studied, the most marked sex differences were found in alpha-glycerophosphate dehydrogenase activity. In control female rats an intense reaction for this enzyme is observed in broad band of cells of the zona fasciculata interna, while in male rats only individual cells showed this reaction. In orchiectomized rats the reaction for this enzyme is similar as in control female rats and testosterone restores it to normal. Ovariectomy has no effect on localisation of reaction while after estradiol replacement reaction was more intense. Regarding the remaining oxidoreductases studied, in the adrenal cortex of rats of both sexes no marked differences were observed in localization, however, intensity of reaction depended upon applied experimental conditions. More distinct sex differences were observed in topochemistry of some hydrolases and intensity of reaction depended upon the applied experimental conditions.", "contents": "Sex differences in adrenocortical structure and function. V. The effects of postpubertal gonadectomy and gonadal hormone replacement on nuclear-cytoplasmic ratio, morphology and histochemistry of rat adrenal cortex. Studies were carried out on adult rats of Wistar strain. Six weeks after postpubertal gonadectomy some of the orchiectomized rats were injected with a single dose of testosterone cypionate and ovari-ctomized rats with estradiol cypionate (17 beta-cyclopentyloproprionate esters of testosterone or estradiol). The control rats were sham operated. Investigations were carried out 8 weeks after surgery. Absolute and relative adrenal weight is lower in the male than in the female rat. Orchiectomy increases these weights while testosterone restores them to their normal level. Ovariectomy has no effect on the weight of adrenal, estradiol, however, increases the relative weight of the gland. The adrenal cortex of the adult female is wider than in the adult male rat and in female gland sudanophobic zone is lacking. Orchiectomy leads to the broadening of the adrenal cortex and testosterone replacement has an opposite effect. Ovariectomy has no effect on the structure of adrenal cortex and estradiol replacement resulted in the narrowing of zona glomerulosa. There were no differences in the nuclear-cytoplasmic ratio of zona glomerulosa cells in male and Female rats and neither gonadectomy nor gonadal hormone replacement has no effect on this parameter. The nuclear-cytoplasmic ratio of zona fasciculata cells in the male is markedly higher than in female. Orchiectomy lowers this ratio and testosterone restores it to the normal level. Neither ovariectomy nor estradiol replacement has effect on the nuclear-cytoplasmic ratio of zona fasciculata cells. Similar changes as those in the zona fasciculata were observed in zona reticularis cells. Among the oxidoreductases studied, the most marked sex differences were found in alpha-glycerophosphate dehydrogenase activity. In control female rats an intense reaction for this enzyme is observed in broad band of cells of the zona fasciculata interna, while in male rats only individual cells showed this reaction. In orchiectomized rats the reaction for this enzyme is similar as in control female rats and testosterone restores it to normal. Ovariectomy has no effect on localisation of reaction while after estradiol replacement reaction was more intense. Regarding the remaining oxidoreductases studied, in the adrenal cortex of rats of both sexes no marked differences were observed in localization, however, intensity of reaction depended upon applied experimental conditions. More distinct sex differences were observed in topochemistry of some hydrolases and intensity of reaction depended upon the applied experimental conditions."}
{"id": "PMID:488857", "title": "Histofluorescence studies of central monoamines. Comparison of Falck, cryostat and SPG methods.", "content": "The aim of the presented paper was to compare the Falck-Hillarp histofluoresce method for monoamines with its cryostat modification and the glyoxylic acid SPG method. The sensitivity and specificity of these methods and also the occurence of unspecific fluorescence is discussed.", "contents": "Histofluorescence studies of central monoamines. Comparison of Falck, cryostat and SPG methods. The aim of the presented paper was to compare the Falck-Hillarp histofluoresce method for monoamines with its cryostat modification and the glyoxylic acid SPG method. The sensitivity and specificity of these methods and also the occurence of unspecific fluorescence is discussed."}
{"id": "PMID:488858", "title": "Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic gonadotropic and steroid hormones. Part I: The effect of gonadotropic and steroid hormones on the amount of lipids and activity of dehydrogenases.", "content": "Effects of 17 beta-estradiol, testosterone, progesterone, luteinizing hormone and a combined effect of estradiol and progesterone on the epithelial cells of the bovine oviduct cultured in vitro were investigated. It was found that these cells may transform under the effect of hormones. The effect of the applied hormones on the amount of lipids and activity of the dehydrogenases delta 5 3 beta-OH-SDH and G6P-DH was evident. Cells in vitro most intensely reacted on testosterone and estradiol: these hormones caused an increase of lipids and of enzymatic activity. The cells also reacted to progesterone and the luteinizing hormone which in turn decreased both activity and accumulation of lipids in cells.", "contents": "Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic gonadotropic and steroid hormones. Part I: The effect of gonadotropic and steroid hormones on the amount of lipids and activity of dehydrogenases. Effects of 17 beta-estradiol, testosterone, progesterone, luteinizing hormone and a combined effect of estradiol and progesterone on the epithelial cells of the bovine oviduct cultured in vitro were investigated. It was found that these cells may transform under the effect of hormones. The effect of the applied hormones on the amount of lipids and activity of the dehydrogenases delta 5 3 beta-OH-SDH and G6P-DH was evident. Cells in vitro most intensely reacted on testosterone and estradiol: these hormones caused an increase of lipids and of enzymatic activity. The cells also reacted to progesterone and the luteinizing hormone which in turn decreased both activity and accumulation of lipids in cells."}
{"id": "PMID:488859", "title": "Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic, gonadotropic and steroid hormones. Part II: The effect of gonadotropic hormones on the amount of glycogen and of acid and alkaline phosphatases.", "content": "The effect of certain gonadotropic and steroid hormones on the epithelial cells of the bovine cultured in vitro was investigated. The effect of the hormones on the activity of acid and alkaline phosphatases was observed. Application of hormones to the medium caused an increase of the amount of glycogen in cells. The activity of alkaline phosphatase increased mostly after application of estradiol (0.2 microgram), progesterone (2 microgram) and the luteinizing hormone (0.1 microgram), and the activity of acid phosphatase after the minimum dose of the luteinizing hormone and estradiol, and the maximum dose of progesterone. On the basis of morphological and histochemical observations, it was assumed that the epithelial cells of the bovine oviduct cultured in vitro may undergo transformations.", "contents": "Reactivity of the epithelial cells of the bovine oviduct in vitro on the exogenic, gonadotropic and steroid hormones. Part II: The effect of gonadotropic hormones on the amount of glycogen and of acid and alkaline phosphatases. The effect of certain gonadotropic and steroid hormones on the epithelial cells of the bovine cultured in vitro was investigated. The effect of the hormones on the activity of acid and alkaline phosphatases was observed. Application of hormones to the medium caused an increase of the amount of glycogen in cells. The activity of alkaline phosphatase increased mostly after application of estradiol (0.2 microgram), progesterone (2 microgram) and the luteinizing hormone (0.1 microgram), and the activity of acid phosphatase after the minimum dose of the luteinizing hormone and estradiol, and the maximum dose of progesterone. On the basis of morphological and histochemical observations, it was assumed that the epithelial cells of the bovine oviduct cultured in vitro may undergo transformations."}
{"id": "PMID:488860", "title": "Vincristine induced ultrastructural alterations of cardiac and smooth muscles in mice.", "content": "A morphological analysis of the effect of vincristine (VCR) on the cardiac muscle and smooth muscles of blood-vessels and alimentary canal of mice has been carried out. These animals show a high resistance to sublethal doses of VCR. The changes in the cardiac muscle involve mitochondria. The widening of the space between the intercalated discs is seen and accompanied by the occurrence of spheromembranous bodies (SMB) in it. The only alteration typical of the effect of VCR in the smooth muscles is the presence of SMB inside the mitochondria. The number and extent of changes observed increased in course of experiment.", "contents": "Vincristine induced ultrastructural alterations of cardiac and smooth muscles in mice. A morphological analysis of the effect of vincristine (VCR) on the cardiac muscle and smooth muscles of blood-vessels and alimentary canal of mice has been carried out. These animals show a high resistance to sublethal doses of VCR. The changes in the cardiac muscle involve mitochondria. The widening of the space between the intercalated discs is seen and accompanied by the occurrence of spheromembranous bodies (SMB) in it. The only alteration typical of the effect of VCR in the smooth muscles is the presence of SMB inside the mitochondria. The number and extent of changes observed increased in course of experiment."}
{"id": "PMID:488861", "title": "Inheritance of sensitivity to cadmium induced testicular damage in mice.", "content": "Testicular damage induced by cadmium in inbred mouse strains was evaluated on the basis of the thickness of tunica albuginea and histological images of the seminiferous tubules and the interstitial tissue. While comparing the thickness of the tunica in the untreated KP, C57 and CBA males as well as in F1 (KP x CBA) and B1 (KP x CBA) x CBA, the authors found it to be inherited in a simple Mendelian way. The thin tunica of the sensitive KP and C57 strain males became markedly thickened after injected with cadmium chloride. On the contrary, no changes were observed in the resistant CBA strain males which are characterised by a thick tunica. The results corroborate Taylor's [10] hypothesis that resistance to the damaging effect of cadmium is controlled by a single autosomal recessive gene. In addition, it is suggested that thickness of the tunica is positively correlated with the resistance to the action of cadmium.", "contents": "Inheritance of sensitivity to cadmium induced testicular damage in mice. Testicular damage induced by cadmium in inbred mouse strains was evaluated on the basis of the thickness of tunica albuginea and histological images of the seminiferous tubules and the interstitial tissue. While comparing the thickness of the tunica in the untreated KP, C57 and CBA males as well as in F1 (KP x CBA) and B1 (KP x CBA) x CBA, the authors found it to be inherited in a simple Mendelian way. The thin tunica of the sensitive KP and C57 strain males became markedly thickened after injected with cadmium chloride. On the contrary, no changes were observed in the resistant CBA strain males which are characterised by a thick tunica. The results corroborate Taylor's [10] hypothesis that resistance to the damaging effect of cadmium is controlled by a single autosomal recessive gene. In addition, it is suggested that thickness of the tunica is positively correlated with the resistance to the action of cadmium."}
{"id": "PMID:488862", "title": "Changes in the activity of RNA polymerase detected in situ and the intensity of 3H uridine incorporation into the nucleolus and the nucleus of interphase cells in antheridial filaments of Chara vulgaris L.", "content": "Analogously as in the 32-cell stage [20], in the 4-, and 8-cell generations of antheridial filaments of Chara vulgaris, the activity of DNA dependent RNA polymerase detected in situ as well as the 3H uridine incorporation increase in the middle S phase and in the middle G2 phase, while they decrease considerably at the end of S phase and in late G2 phase. The diphasic changes occur both in the nucleolar and extranucleolar (nucleoplasmic) activity of RNA polymerase as well as in the 3H uridine incorporation. However, the maximum nucleolar activity, in both S and G2 phases, precedes the peak of nucleoplasmic activity. During the increased nucleolar activity RNA polymerase (as calculated per nucleus) shows a higher level as compared with nucleoplasmic RNA polymerase, whereas the intensity of 3H uridine incorporation into nucleous and nucleoplasm is similar. It may be supposed that the incubation environment containing Mg2+ used in vitro is more stimulating for the nucleolar RNA polymerase than for the nucleoplasmic RNA polymerase. The mean transcriptional activity of the nucleus and the activity of RNA polymerase in the 8-cell generation is about 20% lower than in the 4-cell generation, in proportion to the decrease in cell sizes.", "contents": "Changes in the activity of RNA polymerase detected in situ and the intensity of 3H uridine incorporation into the nucleolus and the nucleus of interphase cells in antheridial filaments of Chara vulgaris L. Analogously as in the 32-cell stage [20], in the 4-, and 8-cell generations of antheridial filaments of Chara vulgaris, the activity of DNA dependent RNA polymerase detected in situ as well as the 3H uridine incorporation increase in the middle S phase and in the middle G2 phase, while they decrease considerably at the end of S phase and in late G2 phase. The diphasic changes occur both in the nucleolar and extranucleolar (nucleoplasmic) activity of RNA polymerase as well as in the 3H uridine incorporation. However, the maximum nucleolar activity, in both S and G2 phases, precedes the peak of nucleoplasmic activity. During the increased nucleolar activity RNA polymerase (as calculated per nucleus) shows a higher level as compared with nucleoplasmic RNA polymerase, whereas the intensity of 3H uridine incorporation into nucleous and nucleoplasm is similar. It may be supposed that the incubation environment containing Mg2+ used in vitro is more stimulating for the nucleolar RNA polymerase than for the nucleoplasmic RNA polymerase. The mean transcriptional activity of the nucleus and the activity of RNA polymerase in the 8-cell generation is about 20% lower than in the 4-cell generation, in proportion to the decrease in cell sizes."}
{"id": "PMID:488863", "title": "Dry mass content in nuclei and cytoplasm during differentiation of the root cortex cells of Helianthus Annuus.", "content": "Dry mass content in nuclei and cytoplasm as well as its concentration during the growth and differentiation of the root cortex cells of Helianthus annuus were investigated by means of interference microscopy. Moreover, by incubation with radioactive thymidine, the endopolyploidization at the level of 4C was found to exist in this species. It was found that during the growth and differentiation of the root cortex cells, the average dry mass content in nuclei essentially does not increase, whereas its content in cytoplasm increases significantly. At the same time the progressive decrease of dry mass concentration takes place in both nuclei and cytoplasm. This drop is connected with their growth and results from the considerable hydratation of karyoplasm and cytoplasm.", "contents": "Dry mass content in nuclei and cytoplasm during differentiation of the root cortex cells of Helianthus Annuus. Dry mass content in nuclei and cytoplasm as well as its concentration during the growth and differentiation of the root cortex cells of Helianthus annuus were investigated by means of interference microscopy. Moreover, by incubation with radioactive thymidine, the endopolyploidization at the level of 4C was found to exist in this species. It was found that during the growth and differentiation of the root cortex cells, the average dry mass content in nuclei essentially does not increase, whereas its content in cytoplasm increases significantly. At the same time the progressive decrease of dry mass concentration takes place in both nuclei and cytoplasm. This drop is connected with their growth and results from the considerable hydratation of karyoplasm and cytoplasm."}
{"id": "PMID:488864", "title": "Histochemical observation of the cortical zone of oocyte of Indian wall lizard, Hemidactylus flaviviridis (R\u00fcppel).", "content": "The cortical zone of oocytes, which lies just below the follicular epithelium appears in the early stages of development, but reaches its fullest growth in vitellogenic oocytes. In the present studies it was found that the cortical zone of Hemidactylus flaviviridis consists of proteins, lipoproteins, carbohydrates, fatty yolk, RNA and little amount of DNA in mature oocytes along with mitochondria and Golgi bodies. In the early oocyte, this zone is fine granular in nature, but during the yolky stages of oocyte, it becomes filled by the vacuolar structure, which shows in it's the presence of fatty and compound yolk. The L1 and L2 types of lipid globules are also observed in the cortical zone during vitellogenic oocyte.", "contents": "Histochemical observation of the cortical zone of oocyte of Indian wall lizard, Hemidactylus flaviviridis (R\u00fcppel). The cortical zone of oocytes, which lies just below the follicular epithelium appears in the early stages of development, but reaches its fullest growth in vitellogenic oocytes. In the present studies it was found that the cortical zone of Hemidactylus flaviviridis consists of proteins, lipoproteins, carbohydrates, fatty yolk, RNA and little amount of DNA in mature oocytes along with mitochondria and Golgi bodies. In the early oocyte, this zone is fine granular in nature, but during the yolky stages of oocyte, it becomes filled by the vacuolar structure, which shows in it's the presence of fatty and compound yolk. The L1 and L2 types of lipid globules are also observed in the cortical zone during vitellogenic oocyte."}
{"id": "PMID:488865", "title": "Spectrophotometric and cytochemical studies on azures A, B and C.", "content": "The cytochemical use of azures A, B and C, propared with either N HCL and potassium metabisulphite or with sodium hydrosulphite in tissue sections were investigated. Both in situ absorption curves of nuclei stained with each of these dye-SO2 reagents as well as in vitro absorption data of acqueous solutions of the dyes are also presented. It has been pointed out that the mechanism of staining with azure A-SO2 and azure C Eosinate-SO2 is the same as that of the conventional Feulgen reaction with Schiff reagent but that of staining with azure B-SO2 is by the modified Feulgen reaction because this dye does not contain any primary amino group.", "contents": "Spectrophotometric and cytochemical studies on azures A, B and C. The cytochemical use of azures A, B and C, propared with either N HCL and potassium metabisulphite or with sodium hydrosulphite in tissue sections were investigated. Both in situ absorption curves of nuclei stained with each of these dye-SO2 reagents as well as in vitro absorption data of acqueous solutions of the dyes are also presented. It has been pointed out that the mechanism of staining with azure A-SO2 and azure C Eosinate-SO2 is the same as that of the conventional Feulgen reaction with Schiff reagent but that of staining with azure B-SO2 is by the modified Feulgen reaction because this dye does not contain any primary amino group."}
{"id": "PMID:488866", "title": "Feeding, foraging, and food sharing behavior of immature chimpanzees.", "content": "Observations of chimpanzees under naturalistic conditions show that nutritional autonomy increases as young individuals mature. Substantial proportions of feeding time are spent eating food obtained through solicitation. Food sharing at SOPF occurs among mother and infant pairs most frequently; less frequently between adult males and infants or juveniles, and least frequently between adult females and immature individuals. Mothers tend to share what their offspring cannot obtain independently while others primarily share what is easiest to replace. These patterns of solicitation and distribution of food are consistent with predictions based upon benefits to individuals through kin selection and/or energetic efficiency.", "contents": "Feeding, foraging, and food sharing behavior of immature chimpanzees. Observations of chimpanzees under naturalistic conditions show that nutritional autonomy increases as young individuals mature. Substantial proportions of feeding time are spent eating food obtained through solicitation. Food sharing at SOPF occurs among mother and infant pairs most frequently; less frequently between adult males and infants or juveniles, and least frequently between adult females and immature individuals. Mothers tend to share what their offspring cannot obtain independently while others primarily share what is easiest to replace. These patterns of solicitation and distribution of food are consistent with predictions based upon benefits to individuals through kin selection and/or energetic efficiency."}
{"id": "PMID:488867", "title": "Entotympanics, ontogeny and primates.", "content": "Ontogenetic investigations confirm that independent entotympanics are absent in living primates. Although cartilage occurs in the petrosal tympanic processes of some primates, the assumption that a suppressed entotympanic is thereby indicated can be adequately refuted according to embryological canons of interpretation. Problems regarding the homologies of different entotympanics, largely ignored by paleontologists and systematists, reduce or negate their taxonomic valency for all but closely related groups. Until such puzzles are resolved, the possible but doubtful existence of entotympanics in plesiadapoids and inferred pre-primate ancestors cannot buttress claims for alleged ties between primates and certain entotympanic-bearing eutherians (principally bats, colugos and tree shrews).", "contents": "Entotympanics, ontogeny and primates. Ontogenetic investigations confirm that independent entotympanics are absent in living primates. Although cartilage occurs in the petrosal tympanic processes of some primates, the assumption that a suppressed entotympanic is thereby indicated can be adequately refuted according to embryological canons of interpretation. Problems regarding the homologies of different entotympanics, largely ignored by paleontologists and systematists, reduce or negate their taxonomic valency for all but closely related groups. Until such puzzles are resolved, the possible but doubtful existence of entotympanics in plesiadapoids and inferred pre-primate ancestors cannot buttress claims for alleged ties between primates and certain entotympanic-bearing eutherians (principally bats, colugos and tree shrews)."}
{"id": "PMID:488876", "title": "[Possibilities for weight reduction by means of diet].", "content": "The different dietetic measures for weight reduction are described. According to the existing overweight the therapeutic measures are classified in four steps. In the first step, with low overweight, the energy-containing drinks (soft drinks and alcoholic beverages) are avoided. If the overweight is greater an additional reduction of the energy content of meal is required. A real reduction-diet (less than 1.000 Kcal/day or 4.200 KJ/day) demands extensive knowledge of food composition and greater efforts in meal composition. The availability of formula diets is considered as a relief. During starvation (or total fasting) as the step 4 of weight reduction diet, an extreme metabolic alteration takes place, which is characterized by ketosis. The same metabolic alteration is found by a fat-protein-diet (a so-called ketogenic diet), where hypercholesterolemia and hyperuricemia are common side effects. The carbohydrate-protein weight reduction diet is poor in health risks. Furthermore the normal metabolic pattern is maintained during this kind of diet if enough carbohydrates are provided per day (i.e. 80-100 g/day).", "contents": "[Possibilities for weight reduction by means of diet]. The different dietetic measures for weight reduction are described. According to the existing overweight the therapeutic measures are classified in four steps. In the first step, with low overweight, the energy-containing drinks (soft drinks and alcoholic beverages) are avoided. If the overweight is greater an additional reduction of the energy content of meal is required. A real reduction-diet (less than 1.000 Kcal/day or 4.200 KJ/day) demands extensive knowledge of food composition and greater efforts in meal composition. The availability of formula diets is considered as a relief. During starvation (or total fasting) as the step 4 of weight reduction diet, an extreme metabolic alteration takes place, which is characterized by ketosis. The same metabolic alteration is found by a fat-protein-diet (a so-called ketogenic diet), where hypercholesterolemia and hyperuricemia are common side effects. The carbohydrate-protein weight reduction diet is poor in health risks. Furthermore the normal metabolic pattern is maintained during this kind of diet if enough carbohydrates are provided per day (i.e. 80-100 g/day)."}
{"id": "PMID:488877", "title": "[Therapy of gout].", "content": "Increasing incidence of gout and its importance as a risk factor for arteriosclerosis stimulate the practitioner to improve his knowledge about therapeutic possibilities. Therapy of gout includes (1) treatment of acute attack with colchicine and indometacin, (2) decrease of uric acid blood concentration in tophaceous gout using uricosurica, alkalinization of urin, increased diuresis, and blockade of de-novo synthesis of uric acid, and (3) maintenance therapy with allopurinol or uricosurica to avoid recurrent arthritis as well as tophi. In addition, surgical removal of larger tophi may be of benefit.", "contents": "[Therapy of gout]. Increasing incidence of gout and its importance as a risk factor for arteriosclerosis stimulate the practitioner to improve his knowledge about therapeutic possibilities. Therapy of gout includes (1) treatment of acute attack with colchicine and indometacin, (2) decrease of uric acid blood concentration in tophaceous gout using uricosurica, alkalinization of urin, increased diuresis, and blockade of de-novo synthesis of uric acid, and (3) maintenance therapy with allopurinol or uricosurica to avoid recurrent arthritis as well as tophi. In addition, surgical removal of larger tophi may be of benefit."}
{"id": "PMID:488879", "title": "[Diabetic changes in the blood vessels of the iris. A fluorescence angiographic study].", "content": "Fluorescence angiography of the iris was performed on 135 patients with diabetes mellitus. The changes in the iris were classified as iridopathia diabetica simplex and iridopathia diabetica proliferans, corresponding to similar changes in diabetic retinopathy. Thus punctata leakage of dye, especially around the pupillary margin, was classified as iridopathia diabetica simplex, whereas additional, angiographically verified new vessel formation was described as iridopathia diabetica proliferans. 123 of 135 patients (91.1%) showed diabetic changes of the iris. Of these 85 (62.9%) had iridopathia diabetica simplex changes and 38 (28.2%) had iridopathia diabetica proliferans changes. The incidence of diabetic iris changes appeared unrelated to age but appeared to increase with duration of diabetes. A correlation between the onset of diabetic vascular changes of the iris and of the retina could not be confirmed: the diabetic changes in the iris vessels can precede those of the retina whereas, on the other hand, almost all patients with a diabetic retinopathia also show an iridopathy. Iris angiography is of clinical value because first signs of diabetic vascular damage can be seen in the iris earlier than in the retina, the iris appearing normal on slit-lamp microscopy.", "contents": "[Diabetic changes in the blood vessels of the iris. A fluorescence angiographic study]. Fluorescence angiography of the iris was performed on 135 patients with diabetes mellitus. The changes in the iris were classified as iridopathia diabetica simplex and iridopathia diabetica proliferans, corresponding to similar changes in diabetic retinopathy. Thus punctata leakage of dye, especially around the pupillary margin, was classified as iridopathia diabetica simplex, whereas additional, angiographically verified new vessel formation was described as iridopathia diabetica proliferans. 123 of 135 patients (91.1%) showed diabetic changes of the iris. Of these 85 (62.9%) had iridopathia diabetica simplex changes and 38 (28.2%) had iridopathia diabetica proliferans changes. The incidence of diabetic iris changes appeared unrelated to age but appeared to increase with duration of diabetes. A correlation between the onset of diabetic vascular changes of the iris and of the retina could not be confirmed: the diabetic changes in the iris vessels can precede those of the retina whereas, on the other hand, almost all patients with a diabetic retinopathia also show an iridopathy. Iris angiography is of clinical value because first signs of diabetic vascular damage can be seen in the iris earlier than in the retina, the iris appearing normal on slit-lamp microscopy."}
{"id": "PMID:488880", "title": "[Effect of preoperative paromomycin therapy on the course of wound healing after colonic intervention. A prospective blind study].", "content": "156 patients undersent colorectal surgery during a prospective blind study at the St\u00e4dt. Krankenhaus M\u00fcnchen-Harlaching. The study showed that administration of 4,0 g Paromomycin (= Humatin) on each of the two preoperative days combined with mechanical cleansing of the intestine has a beneficial effect. Our test group showed significantly lower incidence of wound complications at the abdominal laparotomies (p less than 0,05; X2) and at the anastomoses (p less than 0,01; X2 than the control group. Anastomic leakage (major leaks) and following septical complications occurred nearly exclusively in our \"Not-Humatin-group\". The lethality of the entire group was 13,1% (n = 20).", "contents": "[Effect of preoperative paromomycin therapy on the course of wound healing after colonic intervention. A prospective blind study]. 156 patients undersent colorectal surgery during a prospective blind study at the St\u00e4dt. Krankenhaus M\u00fcnchen-Harlaching. The study showed that administration of 4,0 g Paromomycin (= Humatin) on each of the two preoperative days combined with mechanical cleansing of the intestine has a beneficial effect. Our test group showed significantly lower incidence of wound complications at the abdominal laparotomies (p less than 0,05; X2) and at the anastomoses (p less than 0,01; X2 than the control group. Anastomic leakage (major leaks) and following septical complications occurred nearly exclusively in our \"Not-Humatin-group\". The lethality of the entire group was 13,1% (n = 20)."}
{"id": "PMID:488887", "title": "[Antibiotic-induced pseudomembranous Clostridium difficile colitis. A new etiopathogenetically definable infectious disease].", "content": "Pseudomembranous colitis has been recognized as a complication of antimicrobial therapy since 1952. Most recently, evidence has been accumulated showing that a heat labile toxin is involved. Though little is known so far about the normal ecology of C. difficile and the host factors of potential importance in the development of colitis by this anaerobe, antimicrobial agent-induced suppression of the normal gut flora seems to be a major factor leading to the intestinal proliferation of resistant toxinogenic C. difficile. Factors independent of susceptibility are, however, probably responsible for cases of pseudomembranous colitis associated with penicillin, ampicillin, and other antibiotics active against C. difficile. In monitoring antibiotic therapy, the application of selective media for the isolation of C. difficile from faecal specimens and proper tools for the demonstration of toxin and the study of immunity might prove to be fruitful from both a diagnostic and therapeutic viewpoint. In established pseudomembranous colitis treatment consists of oral vancomycin (2 g/day), cholestyramine and, if necessary, intensive intravenous regimen.", "contents": "[Antibiotic-induced pseudomembranous Clostridium difficile colitis. A new etiopathogenetically definable infectious disease]. Pseudomembranous colitis has been recognized as a complication of antimicrobial therapy since 1952. Most recently, evidence has been accumulated showing that a heat labile toxin is involved. Though little is known so far about the normal ecology of C. difficile and the host factors of potential importance in the development of colitis by this anaerobe, antimicrobial agent-induced suppression of the normal gut flora seems to be a major factor leading to the intestinal proliferation of resistant toxinogenic C. difficile. Factors independent of susceptibility are, however, probably responsible for cases of pseudomembranous colitis associated with penicillin, ampicillin, and other antibiotics active against C. difficile. In monitoring antibiotic therapy, the application of selective media for the isolation of C. difficile from faecal specimens and proper tools for the demonstration of toxin and the study of immunity might prove to be fruitful from both a diagnostic and therapeutic viewpoint. In established pseudomembranous colitis treatment consists of oral vancomycin (2 g/day), cholestyramine and, if necessary, intensive intravenous regimen."}
{"id": "PMID:488897", "title": "[Influences of food browning on nutrition. I. Effects of alcohol extract of egg plant on growth and plasma lipid composition in the rat (author's transl)].", "content": "Browning substances are produced on incubation of minced or finely crushed egg plant in alcohol for various periods (from 3 to 12 months). Effects of chronic ingestion of the browning substances were studied on growth rate, general nutrition, and plasma lipids, especially on cholesterol and fatty acid composition in five groups of rats. Control rats (the sixth group) were fed standard diet and water ad libitum. 1. The browning substances reduced the growth rate and nutritional efficiency in the rat. 2. This inhibitory effect was more marked when rats were fed the diet containing advanced browning substances on the longest incubation of 12 months. 3. Ingestion of the browning substances of long incubation caused an elevation of the serum cholesterol level as compared with controls. 4. Analysis of serum fatty acid composition showed in general a reduction in unsaturated fatty acid and an increase in saturated fatty acid, especially in palmitic acid after ingestion of browning substances. 5. Ingestion of lyophilized powder of fresh egg plants also caused inhibition of growth rate and nutritional efficiency.", "contents": "[Influences of food browning on nutrition. I. Effects of alcohol extract of egg plant on growth and plasma lipid composition in the rat (author's transl)]. Browning substances are produced on incubation of minced or finely crushed egg plant in alcohol for various periods (from 3 to 12 months). Effects of chronic ingestion of the browning substances were studied on growth rate, general nutrition, and plasma lipids, especially on cholesterol and fatty acid composition in five groups of rats. Control rats (the sixth group) were fed standard diet and water ad libitum. 1. The browning substances reduced the growth rate and nutritional efficiency in the rat. 2. This inhibitory effect was more marked when rats were fed the diet containing advanced browning substances on the longest incubation of 12 months. 3. Ingestion of the browning substances of long incubation caused an elevation of the serum cholesterol level as compared with controls. 4. Analysis of serum fatty acid composition showed in general a reduction in unsaturated fatty acid and an increase in saturated fatty acid, especially in palmitic acid after ingestion of browning substances. 5. Ingestion of lyophilized powder of fresh egg plants also caused inhibition of growth rate and nutritional efficiency."}
{"id": "PMID:488898", "title": "[Influences of food browning on nutrition. II. Effects of ingestion of non-dialyzable browning substances, dopamelanin and chlorogenic acid on growth and protein and lipid metabolism in rats (author's transl)].", "content": "In a previous paper, we reported an inhibitory effect on growth rate and plasma lipid composition in rats of browning substances obtained from ethanol extraction of minced egg plant. In order to know active principle(s) in browning substances, attempts were made to condense the substances and chlorogenic acid was separated. The ingestion of chlorogenic acid and other browning substances such as dopameranin and catechin were examined for their biological activity. Active principle(s) in the browning substances appeared to interfere with digestion and utilization of dietic protein. The principle resided in dialyzable fraction in enzymatically produced materials and in nondialyzable fraction in materials obtained by chemical oxidation. Ingestion of nondialyzable fraction caused an elevation of total serum cholesterol level, but little change in serum fatty acid composition.", "contents": "[Influences of food browning on nutrition. II. Effects of ingestion of non-dialyzable browning substances, dopamelanin and chlorogenic acid on growth and protein and lipid metabolism in rats (author's transl)]. In a previous paper, we reported an inhibitory effect on growth rate and plasma lipid composition in rats of browning substances obtained from ethanol extraction of minced egg plant. In order to know active principle(s) in browning substances, attempts were made to condense the substances and chlorogenic acid was separated. The ingestion of chlorogenic acid and other browning substances such as dopameranin and catechin were examined for their biological activity. Active principle(s) in the browning substances appeared to interfere with digestion and utilization of dietic protein. The principle resided in dialyzable fraction in enzymatically produced materials and in nondialyzable fraction in materials obtained by chemical oxidation. Ingestion of nondialyzable fraction caused an elevation of total serum cholesterol level, but little change in serum fatty acid composition."}
{"id": "PMID:488900", "title": "[Studies on lysosomes in hepatic disorders (author's transl)].", "content": "Lysosome are subcellular particles in which several acid hydrolases of various specificities are localized. The role of lysosome in cellular physiology and pathology has drawn considerable recent attention by several groups of investigators. The purpose of this study was to investigate the activities of lysosomal enzymes--acid phosphatase, beta-glucuronidase, N-acetyl-beta-glucosaminidase--in hepatic disorders. 1) The serum levels of beta-glucuronidase and N-acetyl-beta-glucosaminidase were significantly elevated in patients with diseases of the hepatobiliary system. 2) N-acetyl-beta-glucosaminidase activity in urine specimens from patients with diseases of the hepatobiliary system was found to be significantly higher than in urine specimens from normal adults. 3) Male albino rats of 150 approximately 200 g body weight were used. CCl4 was injected intraperitoneally (dose 0.1 ml of CCl4 per 100 g body weight twice a week for eight weeks). The free activities of lysosomal enzyme were increased and high free/total activity ratios were found in the liver lysosomal fraction of CCl4 intoxicated rats. The results of these experiment indicated that the membranes of lysosome were more permeable to their enzymes, and the release of these enzymes were found in the experimental fatty liver by CCl4. 4) Corticosteroids and chloroquine stabilized rat liver lysosome in vitro from the labilizing influence of incubation at 37 degrees C. 5) The administration of chloroquine to CCl4 intoxicated rats did not cause any well-expressed stabilization of lysosomes. 6) When alpha-Tocopherol was administrated to CCl4 intoxicated rats, the decrease of bound activity and increase of free activity in lysosomal fraction, and increase of acid hydrolases, GOT and GPT in serum were inhibited.", "contents": "[Studies on lysosomes in hepatic disorders (author's transl)]. Lysosome are subcellular particles in which several acid hydrolases of various specificities are localized. The role of lysosome in cellular physiology and pathology has drawn considerable recent attention by several groups of investigators. The purpose of this study was to investigate the activities of lysosomal enzymes--acid phosphatase, beta-glucuronidase, N-acetyl-beta-glucosaminidase--in hepatic disorders. 1) The serum levels of beta-glucuronidase and N-acetyl-beta-glucosaminidase were significantly elevated in patients with diseases of the hepatobiliary system. 2) N-acetyl-beta-glucosaminidase activity in urine specimens from patients with diseases of the hepatobiliary system was found to be significantly higher than in urine specimens from normal adults. 3) Male albino rats of 150 approximately 200 g body weight were used. CCl4 was injected intraperitoneally (dose 0.1 ml of CCl4 per 100 g body weight twice a week for eight weeks). The free activities of lysosomal enzyme were increased and high free/total activity ratios were found in the liver lysosomal fraction of CCl4 intoxicated rats. The results of these experiment indicated that the membranes of lysosome were more permeable to their enzymes, and the release of these enzymes were found in the experimental fatty liver by CCl4. 4) Corticosteroids and chloroquine stabilized rat liver lysosome in vitro from the labilizing influence of incubation at 37 degrees C. 5) The administration of chloroquine to CCl4 intoxicated rats did not cause any well-expressed stabilization of lysosomes. 6) When alpha-Tocopherol was administrated to CCl4 intoxicated rats, the decrease of bound activity and increase of free activity in lysosomal fraction, and increase of acid hydrolases, GOT and GPT in serum were inhibited."}
{"id": "PMID:488901", "title": "[Neural mechanisms of group I fibers of neck muscles (author's transl)].", "content": "Afferent projection of upper cervical dorsal roots C2 and C3 upon spinal cord and brain stem was investigated on cats using both histological and electrophysiological methods. 1. Degenerated terminals, stained by Fink-Heimer's methods, were found in Rexed's laminae III and IV, intermediate nucleus and ventral horn of the spinal cord and nucleus cuneatus and spinal trigeminal nucleus. 2. Monosynaptic reflex responses were obtained from motor nerves innervating dorsal neck muscles by stimulating the dorsal roots in some decerebrated cats. However, this reflex responses could be unable to elicite in the spinal preparation. 3. The neurons responding exclusively to the ventral rami located in cranial parts of the nucleus and those responding to dorsal rami located in the caudal region. Neurons responding to both rami were also found.", "contents": "[Neural mechanisms of group I fibers of neck muscles (author's transl)]. Afferent projection of upper cervical dorsal roots C2 and C3 upon spinal cord and brain stem was investigated on cats using both histological and electrophysiological methods. 1. Degenerated terminals, stained by Fink-Heimer's methods, were found in Rexed's laminae III and IV, intermediate nucleus and ventral horn of the spinal cord and nucleus cuneatus and spinal trigeminal nucleus. 2. Monosynaptic reflex responses were obtained from motor nerves innervating dorsal neck muscles by stimulating the dorsal roots in some decerebrated cats. However, this reflex responses could be unable to elicite in the spinal preparation. 3. The neurons responding exclusively to the ventral rami located in cranial parts of the nucleus and those responding to dorsal rami located in the caudal region. Neurons responding to both rami were also found."}
{"id": "PMID:488903", "title": "Effect of cyproterone acetate acutely administered on the pituitary-testicular axis.", "content": "The effect of cyproterone acetate (CA) on the pituitary-testicular axis was studied in 6 healthy men. A dose of 300 mg of CA was administered orally in the early morning, after 3 h blood samples were collected using a multiple sample technique. Testosterone (T) levels were decreased by CA in all subjects (p 0.01), LH in all (p less than 0.01) but one whereas 17-hydroxyprogesterone did not show any significant variation. In vitro, using an equilibrium dialysis, CA displaced T from plasma-binding proteins in males at 37 degrees C. The role of testosterone-binding globulin on the effects of CA on the pituitary-testicular axis remains to be clarified.", "contents": "Effect of cyproterone acetate acutely administered on the pituitary-testicular axis. The effect of cyproterone acetate (CA) on the pituitary-testicular axis was studied in 6 healthy men. A dose of 300 mg of CA was administered orally in the early morning, after 3 h blood samples were collected using a multiple sample technique. Testosterone (T) levels were decreased by CA in all subjects (p 0.01), LH in all (p less than 0.01) but one whereas 17-hydroxyprogesterone did not show any significant variation. In vitro, using an equilibrium dialysis, CA displaced T from plasma-binding proteins in males at 37 degrees C. The role of testosterone-binding globulin on the effects of CA on the pituitary-testicular axis remains to be clarified."}
{"id": "PMID:488904", "title": "Stimulation of gastric mucosal protein synthesis by different molecular forms of gastrin.", "content": "Protein synthesis in gastric mucosa was studied by measuring the incorporation of labeled amino acids into protein by isolated gastric mucosal ribosomes in a cell-free system. In 48-hour fasted rats, administration of the synthetic analogues pentagastrin, tetragastrin and gastrin-17 or naturally occurring molecular forms of human gastrin (G-14, G-34) markedly enhanced (23-123%) the capacity of the gastric mucosal ribosomes to synthesize endogenous mRNA-directed protein in a cell-free system. In the presence of exogenous mRNA (poly-U), the gastric mucosal ribosomes from the saline-treated controls showed a higher poly(U)-directed protein synthesis, compared to each fo the gastrin-treated groups. The protein/polyphenylalanine ratio which represents a ratio of polysomes to monosomes was found increased in ribosomes from the gastrin-treated groups.", "contents": "Stimulation of gastric mucosal protein synthesis by different molecular forms of gastrin. Protein synthesis in gastric mucosa was studied by measuring the incorporation of labeled amino acids into protein by isolated gastric mucosal ribosomes in a cell-free system. In 48-hour fasted rats, administration of the synthetic analogues pentagastrin, tetragastrin and gastrin-17 or naturally occurring molecular forms of human gastrin (G-14, G-34) markedly enhanced (23-123%) the capacity of the gastric mucosal ribosomes to synthesize endogenous mRNA-directed protein in a cell-free system. In the presence of exogenous mRNA (poly-U), the gastric mucosal ribosomes from the saline-treated controls showed a higher poly(U)-directed protein synthesis, compared to each fo the gastrin-treated groups. The protein/polyphenylalanine ratio which represents a ratio of polysomes to monosomes was found increased in ribosomes from the gastrin-treated groups."}
{"id": "PMID:488905", "title": "Gel filtration studies of serum growth hormone in acromegaly following bromocriptine administration.", "content": "Sera of 7 patients with active acromegaly were fractionated by Sephadex G-100 chromatography and the effects of bromocriptine on the concentrations of total growth hormone (hGH) and its different molecular forms studied. Three immunoreactive peaks were observed, corresponding to molecular weights of about 20,000 ('little hGH'), 40,000 ('big hGH'), and more than 100,000 ('big big hGH') Following bromocriptine administration, there was significantly more reduction of 'little hGH' than of 'big big hGH'. Careful interpretation of these changes is required in view of the possible influences of sample storage and handling on hGH heterogeneity. We suggest that either bromocriptine acts differentially on the release of 'little' and 'big big hGH', or that these components differ in their metabolic half-life. However, even the suppression of 'little hGH' is insufficient to explain the clinical response of the disease to bromocriptine.", "contents": "Gel filtration studies of serum growth hormone in acromegaly following bromocriptine administration. Sera of 7 patients with active acromegaly were fractionated by Sephadex G-100 chromatography and the effects of bromocriptine on the concentrations of total growth hormone (hGH) and its different molecular forms studied. Three immunoreactive peaks were observed, corresponding to molecular weights of about 20,000 ('little hGH'), 40,000 ('big hGH'), and more than 100,000 ('big big hGH') Following bromocriptine administration, there was significantly more reduction of 'little hGH' than of 'big big hGH'. Careful interpretation of these changes is required in view of the possible influences of sample storage and handling on hGH heterogeneity. We suggest that either bromocriptine acts differentially on the release of 'little' and 'big big hGH', or that these components differ in their metabolic half-life. However, even the suppression of 'little hGH' is insufficient to explain the clinical response of the disease to bromocriptine."}
{"id": "PMID:488906", "title": "The covalent structure of calf skin type III collagen. I. The amino acid sequence of the amino terminal region of the alpha 1(III) chain (position 1--222).", "content": "The amino terminal 227 amino acid residues of the alpha 1(III) chain contain four CNBr peptides: alpha 1(III)CB3A (79 residues), CB3B, CB3C (6 residues each), CB7 (37 residues) and CB6 (99 residues). Fragmentation of the CNBr peptides was carried out using trypsin, chymotrypsin and the protease from Staphylococcus aureus V8. The fragments obtained were isolated by a combination of molecular sieve and ion exchange chromatography. The sequence analysis was performed according to the automated Edman degradation procedure.", "contents": "The covalent structure of calf skin type III collagen. I. The amino acid sequence of the amino terminal region of the alpha 1(III) chain (position 1--222). The amino terminal 227 amino acid residues of the alpha 1(III) chain contain four CNBr peptides: alpha 1(III)CB3A (79 residues), CB3B, CB3C (6 residues each), CB7 (37 residues) and CB6 (99 residues). Fragmentation of the CNBr peptides was carried out using trypsin, chymotrypsin and the protease from Staphylococcus aureus V8. The fragments obtained were isolated by a combination of molecular sieve and ion exchange chromatography. The sequence analysis was performed according to the automated Edman degradation procedure."}
{"id": "PMID:488907", "title": "The covalent structure of calf skin type III collagen. II. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB1,8,10,2(Positions 223--402).", "content": "The cyanogen bromide peptide alpha 1-(III)CB1,8,10,2 is 180 amino acid residues in length and occupies position 223 to 402 along the alpha 1(III) chain. In order to elucidate its amino acid sequence, alpha 1(III)CB1,8,10,2 was fragmented with hydroxylamine, protease from Staphylococcus aureus V8 and trypsin. Peptides necessary for sequence analysis with the automated Edman degradation were separated using molecular and ion exchange chromatography. Edman degradation of the hydroxylamine-derived fragments resulted in the elucidation of 80% of the entire sequence. The rest was completely established by sequence analysis of some protease V8 and trypsin-derived peptides.", "contents": "The covalent structure of calf skin type III collagen. II. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB1,8,10,2(Positions 223--402). The cyanogen bromide peptide alpha 1-(III)CB1,8,10,2 is 180 amino acid residues in length and occupies position 223 to 402 along the alpha 1(III) chain. In order to elucidate its amino acid sequence, alpha 1(III)CB1,8,10,2 was fragmented with hydroxylamine, protease from Staphylococcus aureus V8 and trypsin. Peptides necessary for sequence analysis with the automated Edman degradation were separated using molecular and ion exchange chromatography. Edman degradation of the hydroxylamine-derived fragments resulted in the elucidation of 80% of the entire sequence. The rest was completely established by sequence analysis of some protease V8 and trypsin-derived peptides."}
{"id": "PMID:488908", "title": "The covalent structure of calf skin type III collagen. III. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB4 (positions 403--551).", "content": "The cyanogen bromide peptide alpha 1(III)CB4 comprises the sequence region from position 403 to 551 of the alpha 1(III) chain. Almost the entire sequence of this region was elucidated using two hydroxylamine- and one chymotrypsin-derived fragments for automated Edman degradation. The sequence analysis of alpha 1(III)CB4 was completed with the help of trypsin and one protease V8-derived peptide. Comparison with the corresponding region of the alpha 1(I) chain revealed a striking homology between the two chains in this region which is higher than for the entire alpha chains.", "contents": "The covalent structure of calf skin type III collagen. III. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB4 (positions 403--551). The cyanogen bromide peptide alpha 1(III)CB4 comprises the sequence region from position 403 to 551 of the alpha 1(III) chain. Almost the entire sequence of this region was elucidated using two hydroxylamine- and one chymotrypsin-derived fragments for automated Edman degradation. The sequence analysis of alpha 1(III)CB4 was completed with the help of trypsin and one protease V8-derived peptide. Comparison with the corresponding region of the alpha 1(I) chain revealed a striking homology between the two chains in this region which is higher than for the entire alpha chains."}
{"id": "PMID:488909", "title": "The covalent structure of calf skin type III collagen. IV. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB5 (positions 552--788).", "content": "The cyanogen bromide peptide alpha 1(III)CB5 is 237 amino acid residues in length and occupies position 552--788 along the alpha 1(III) chain. For sequence analysis alpha 1(III)CB5 was fragmented with hydroxylamine, protease from Staphylococcus aureus V8, trypsin and the arginine-specific enzyme from mouse submaxillary gland. The peptides obtained were separated using molecular and ion exchange chromatography and sequenced with the automated Edman degradation procedure.", "contents": "The covalent structure of calf skin type III collagen. IV. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB5 (positions 552--788). The cyanogen bromide peptide alpha 1(III)CB5 is 237 amino acid residues in length and occupies position 552--788 along the alpha 1(III) chain. For sequence analysis alpha 1(III)CB5 was fragmented with hydroxylamine, protease from Staphylococcus aureus V8, trypsin and the arginine-specific enzyme from mouse submaxillary gland. The peptides obtained were separated using molecular and ion exchange chromatography and sequenced with the automated Edman degradation procedure."}
{"id": "PMID:488910", "title": "The covalent structure of calf skin type III collagen. V. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB9A (position 789 to 927).", "content": "The cyanogen bromide peptide alpha 1(III)CB9A is 139 amino acid residues in length and occupies positions 789--927 along the alpha 1(III) chain. Peptides necessary for the complete sequence analysis were obtained after fragmentation of alpha 1(III)CB9B with trypsin, protease from Staphylococcus aureus V8, hydroxylamine and chymotrypsin. They were separated mainly by chromatography on Sephadex G-50 and phosphocellulose and subsequently sequenced using the automated Edman degradation procedure.", "contents": "The covalent structure of calf skin type III collagen. V. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB9A (position 789 to 927). The cyanogen bromide peptide alpha 1(III)CB9A is 139 amino acid residues in length and occupies positions 789--927 along the alpha 1(III) chain. Peptides necessary for the complete sequence analysis were obtained after fragmentation of alpha 1(III)CB9B with trypsin, protease from Staphylococcus aureus V8, hydroxylamine and chymotrypsin. They were separated mainly by chromatography on Sephadex G-50 and phosphocellulose and subsequently sequenced using the automated Edman degradation procedure."}
{"id": "PMID:488911", "title": "The covalent structure of calf skin type III collagen. VI. The amino acid sequence of the carboxyterminal cyanogen bromide peptide alpha 1(III)CB9B (position 928--1028).", "content": "The C-terminal cyanogen bromide peptide alpha 1(III)CB9B is 101 amino acid residues in length and occupies position 928--1028 along the alpha 1(III) chain. For sequence analysis, alpha 1(III)CB9B was fragmented with trypsin and chymotrypsin. The peptides obtained were separated using molecular sieve and ion exchange chromatography and sequenced using the automated Edman degradation procedure.", "contents": "The covalent structure of calf skin type III collagen. VI. The amino acid sequence of the carboxyterminal cyanogen bromide peptide alpha 1(III)CB9B (position 928--1028). The C-terminal cyanogen bromide peptide alpha 1(III)CB9B is 101 amino acid residues in length and occupies position 928--1028 along the alpha 1(III) chain. For sequence analysis, alpha 1(III)CB9B was fragmented with trypsin and chymotrypsin. The peptides obtained were separated using molecular sieve and ion exchange chromatography and sequenced using the automated Edman degradation procedure."}
{"id": "PMID:488913", "title": "The effect of sodium chloride on the structure of ribonucleoprotein particles from rat liver nuclei.", "content": "38S (monoparticles) and greater than 50--200S ribonucleoprotein particles (polyparticles) from rat liver nuclei were treated with increasing concentrations of sodium chloride. Treatment of 38S or greater than 50--200S particles, with 0.14, 0.25, 0.5, 1.0, and 2.0M NaCl resulted in a decrease of protein to RNA ratios from 8 to 3.1 for 38S particles and from 4.0 to 1.5 for greater than 20--200S particles. Correspondingly the densities in CsCl increased. Whereas the maximum of the sedimentation profile of polyparticles decreased from 90S to 50S after treatment with increasing NaCl concentrations, a discontinuous change was found in the case of monoparticles. It was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis that the proteins which were dissociated by NaCl were in the molecular weight range of 30--45 000. Four of the 5 small molecular weight RNAs in the range of 4.5 to 8S remained tightly associated even after treatment of polyparticles with 2.0M NaCl. When 38S or 70--200S nRNP particles were exposed to increasing concentrations of NaCl (0.25, 0.5, 1.0, 2.0M), the molar ellipticity at 264 nm increased progressively to about 40%. Upon NaCl treatment of polyparticles and successive removal of the dissociated proteins by centrifugation the increase in the positive CD band at 264 nm was only 15%.", "contents": "The effect of sodium chloride on the structure of ribonucleoprotein particles from rat liver nuclei. 38S (monoparticles) and greater than 50--200S ribonucleoprotein particles (polyparticles) from rat liver nuclei were treated with increasing concentrations of sodium chloride. Treatment of 38S or greater than 50--200S particles, with 0.14, 0.25, 0.5, 1.0, and 2.0M NaCl resulted in a decrease of protein to RNA ratios from 8 to 3.1 for 38S particles and from 4.0 to 1.5 for greater than 20--200S particles. Correspondingly the densities in CsCl increased. Whereas the maximum of the sedimentation profile of polyparticles decreased from 90S to 50S after treatment with increasing NaCl concentrations, a discontinuous change was found in the case of monoparticles. It was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis that the proteins which were dissociated by NaCl were in the molecular weight range of 30--45 000. Four of the 5 small molecular weight RNAs in the range of 4.5 to 8S remained tightly associated even after treatment of polyparticles with 2.0M NaCl. When 38S or 70--200S nRNP particles were exposed to increasing concentrations of NaCl (0.25, 0.5, 1.0, 2.0M), the molar ellipticity at 264 nm increased progressively to about 40%. Upon NaCl treatment of polyparticles and successive removal of the dissociated proteins by centrifugation the increase in the positive CD band at 264 nm was only 15%."}
{"id": "PMID:488914", "title": "An improved method for the determination of cytoplasmic 3 alpha- and 17 beta- and microsomal 3 alpha-hydroxysteroid dehydrogenase activities in rat liver.", "content": "This paper described a modified method for the radiometric determination of hydroxysteroid dehydrogenase activities in rat liver. The principle advantages of this method are the improved precision and a radical reduction in the time involved in performing the assay. The procedure comprises the following steps: incubation of 14C-labelled substrate with coenzyme and cell fraction under optimized conditions; termination of the reaction by addition of organic solvent containing a defined amount of 3H-labelled reaction product; removal of precipitated protein and coenzyme by centrifugation; paper chromatographic isolation of the product; direct quantitation of 14C activity in the product zone of the paper chromatogram. The assay systems have been applied to elucidate and quantitate sex and strain differences in the activities of the above enzymes in Chbb/THOM and Sprague-Dawley rats.", "contents": "An improved method for the determination of cytoplasmic 3 alpha- and 17 beta- and microsomal 3 alpha-hydroxysteroid dehydrogenase activities in rat liver. This paper described a modified method for the radiometric determination of hydroxysteroid dehydrogenase activities in rat liver. The principle advantages of this method are the improved precision and a radical reduction in the time involved in performing the assay. The procedure comprises the following steps: incubation of 14C-labelled substrate with coenzyme and cell fraction under optimized conditions; termination of the reaction by addition of organic solvent containing a defined amount of 3H-labelled reaction product; removal of precipitated protein and coenzyme by centrifugation; paper chromatographic isolation of the product; direct quantitation of 14C activity in the product zone of the paper chromatogram. The assay systems have been applied to elucidate and quantitate sex and strain differences in the activities of the above enzymes in Chbb/THOM and Sprague-Dawley rats."}
{"id": "PMID:488915", "title": "Comparative metabolic studies on liver sinusoidal cells and different types of macrophages.", "content": "Sinusoidal cells of rat liver, rat peritoneal macrophages and rabbit alveolar macrophages were checked for their viability and compared with regard to cell weight, protein and DNA content. Glycogen was virtually absent from sinusoidal cells and peritoneal macrophages; alveolar macrophages contained glycogen whose level increased after activation by Freund's adjuvant and decreased during phagocytosis in the absence of glucose. Of the different nucleotides assayed, UDPglucose levels were low in the nonglycogen-forming cells, but quite high in alveolar macrophages. The capacity to metabolize galactose is much smaller in all cell types investigated than in hepatocytes.", "contents": "Comparative metabolic studies on liver sinusoidal cells and different types of macrophages. Sinusoidal cells of rat liver, rat peritoneal macrophages and rabbit alveolar macrophages were checked for their viability and compared with regard to cell weight, protein and DNA content. Glycogen was virtually absent from sinusoidal cells and peritoneal macrophages; alveolar macrophages contained glycogen whose level increased after activation by Freund's adjuvant and decreased during phagocytosis in the absence of glucose. Of the different nucleotides assayed, UDPglucose levels were low in the nonglycogen-forming cells, but quite high in alveolar macrophages. The capacity to metabolize galactose is much smaller in all cell types investigated than in hepatocytes."}
{"id": "PMID:488916", "title": "Molecular forms of purified human erythrocyte membrane acetylcholinesterase investigated by crosslinking with diimidates.", "content": "Several molecular forms of human erythrocyte membrane acetylcholinesterase have been studied after crosslinking with bifunctional diimidates. The crosslinked products were analysed by centrifugation on linear sucrose density gradients containing Triton X-100. Molecular weights of covalently linked oligomers were estimated by sodium dodecylsulfate gel electrophoresis. It was shown that acetylcholinesterase crosslinked in absence of Triton X-100 consists of molecular forms built up by dimeric protomers. These dimers were identical with the enzymatically active species sedimenting with 6.5S in linear sucrose density gradients.", "contents": "Molecular forms of purified human erythrocyte membrane acetylcholinesterase investigated by crosslinking with diimidates. Several molecular forms of human erythrocyte membrane acetylcholinesterase have been studied after crosslinking with bifunctional diimidates. The crosslinked products were analysed by centrifugation on linear sucrose density gradients containing Triton X-100. Molecular weights of covalently linked oligomers were estimated by sodium dodecylsulfate gel electrophoresis. It was shown that acetylcholinesterase crosslinked in absence of Triton X-100 consists of molecular forms built up by dimeric protomers. These dimers were identical with the enzymatically active species sedimenting with 6.5S in linear sucrose density gradients."}
{"id": "PMID:488917", "title": "Studies on plant bile pigments, VII. Preparation and characterization of phycobiliproteins with chromophores chemically modified by reduction.", "content": "The reversible denaturation and reduction with dithionite has been studied for the phycobiliproteins, C-phycocyanin (1) and allophycocyanin (2) from Spirulina platensis, and C-phycoerythrin (4) from Fremyella diplosiphon (both cyanobacteria). By treatment with sodium dithionite, the chromophores are selectively reduced at the central (C-10) methine bridge, producing pigments with bilirubinoid (lambda max = 418 nm from 1 and 2), and vinylpyrroloc (lambda max= 300 nm from 4) chromophores. The extent of reduction is dependent on the state of the protein. The chromophores of denatured biliproteins are completely reduced at 0.5 mM dithionite. In the native pigments, dithionite concentrations up to 0.5 mM lead only to partial reduction, thus forming products containing both reduced and oxidized chromophores (e.g. \"phycocyanorubins\" from 1 and 2). The reduction is non-statistical with respect to the different chromophores present in 1 and 4, the chromophores absorbing at shorter wavelengths being preferentially reduced. Renaturation of the proteins containing reduced chromophores is accompanied by their reoxidation. This oxidation is complete in the absence of dithionite or at concentrations up to 0.5 mM. At higher dithionite concentrations, the reoxidation is incomplete, and the products are spectroscopically identical to those obtained by reduction of the native pigments at similar concentrations of reductant. The results are interpreted by a model in which the protein is \"transparent\" to the reducing agent, dithionite. The difference in the extent of reduction of the native and denatured pigments can only be due to thermodynamic (viz. stability) differences in the susceptibility of the chromophores to reduction. Specifically, the (extended) chromophore present in the native pigment is much more difficult to reduce than the chromophore (present in a cyclic conformation) in the denatured pigment. The energetics of the process of refolding both the protein and the chromophores are discussed.", "contents": "Studies on plant bile pigments, VII. Preparation and characterization of phycobiliproteins with chromophores chemically modified by reduction. The reversible denaturation and reduction with dithionite has been studied for the phycobiliproteins, C-phycocyanin (1) and allophycocyanin (2) from Spirulina platensis, and C-phycoerythrin (4) from Fremyella diplosiphon (both cyanobacteria). By treatment with sodium dithionite, the chromophores are selectively reduced at the central (C-10) methine bridge, producing pigments with bilirubinoid (lambda max = 418 nm from 1 and 2), and vinylpyrroloc (lambda max= 300 nm from 4) chromophores. The extent of reduction is dependent on the state of the protein. The chromophores of denatured biliproteins are completely reduced at 0.5 mM dithionite. In the native pigments, dithionite concentrations up to 0.5 mM lead only to partial reduction, thus forming products containing both reduced and oxidized chromophores (e.g. \"phycocyanorubins\" from 1 and 2). The reduction is non-statistical with respect to the different chromophores present in 1 and 4, the chromophores absorbing at shorter wavelengths being preferentially reduced. Renaturation of the proteins containing reduced chromophores is accompanied by their reoxidation. This oxidation is complete in the absence of dithionite or at concentrations up to 0.5 mM. At higher dithionite concentrations, the reoxidation is incomplete, and the products are spectroscopically identical to those obtained by reduction of the native pigments at similar concentrations of reductant. The results are interpreted by a model in which the protein is \"transparent\" to the reducing agent, dithionite. The difference in the extent of reduction of the native and denatured pigments can only be due to thermodynamic (viz. stability) differences in the susceptibility of the chromophores to reduction. Specifically, the (extended) chromophore present in the native pigment is much more difficult to reduce than the chromophore (present in a cyclic conformation) in the denatured pigment. The energetics of the process of refolding both the protein and the chromophores are discussed."}
{"id": "PMID:488918", "title": "[Degradation and biosynthesis of L-phenylalanine by chloridazon-degrading bacteria].", "content": "Incubating chloridazon-degrading bacteria with L-phenylalanine leads to the accumulation of L-2,3-dihydroxyphenylalanine, o-tyrosine and m-tyrosine in the medium. Incubating the bacteria with N-acetyl-L-phenylalanine leads to N-acetyl-(2,3-dihydroxyphenyl)alanine. Using phenylacetic acid as substrate leads to the accumulation of malonic acid. The products are isolated by gel chromatography and high performance liquid chromatography. 2,3-Dihydroxy-L-phenylalanine is attacked by a catechol 2,3-dioxygenase in the presence of Fe2. An unstable yellow compound is formed in this reaction. This meta-cleavage-product is again cleaved by a hydrolase, leading to aspartic acid and 4-hydroxy-2-oxovaleric acid. Both products were isolated fromthe reaction buffer by amino acid analysis and high performance liquid chromatography. The dioxygenase and hydrolase were partially purified and characterized. A new degradation pathway for phenylalanine is discussed and compared with known pathways. The enzymes chorismate mutase, prephenate dehydratase and prephenate dehydrogenase are characterized and inhibition as well as repression are investigated. Only prephenate dehydrogenase is inhibited by phenylalanine, tyrosine and tryptophane. Chorismate mutase is repressed by phenylalanine, prephenate dehydrogenase by phenylalanine and tyrosine. Prephenate dehydratase is not repressed by aromatic amino acids. Regulation of aromatic amino acid biosynthesis in connection with phenylalanine degradation is discussed.", "contents": "[Degradation and biosynthesis of L-phenylalanine by chloridazon-degrading bacteria]. Incubating chloridazon-degrading bacteria with L-phenylalanine leads to the accumulation of L-2,3-dihydroxyphenylalanine, o-tyrosine and m-tyrosine in the medium. Incubating the bacteria with N-acetyl-L-phenylalanine leads to N-acetyl-(2,3-dihydroxyphenyl)alanine. Using phenylacetic acid as substrate leads to the accumulation of malonic acid. The products are isolated by gel chromatography and high performance liquid chromatography. 2,3-Dihydroxy-L-phenylalanine is attacked by a catechol 2,3-dioxygenase in the presence of Fe2. An unstable yellow compound is formed in this reaction. This meta-cleavage-product is again cleaved by a hydrolase, leading to aspartic acid and 4-hydroxy-2-oxovaleric acid. Both products were isolated fromthe reaction buffer by amino acid analysis and high performance liquid chromatography. The dioxygenase and hydrolase were partially purified and characterized. A new degradation pathway for phenylalanine is discussed and compared with known pathways. The enzymes chorismate mutase, prephenate dehydratase and prephenate dehydrogenase are characterized and inhibition as well as repression are investigated. Only prephenate dehydrogenase is inhibited by phenylalanine, tyrosine and tryptophane. Chorismate mutase is repressed by phenylalanine, prephenate dehydrogenase by phenylalanine and tyrosine. Prephenate dehydratase is not repressed by aromatic amino acids. Regulation of aromatic amino acid biosynthesis in connection with phenylalanine degradation is discussed."}
{"id": "PMID:488919", "title": "Studies on the hydrolysis of 1-alk-1'-enyl-sn-glycero-3-phosphoethanolamine by microsomes from myelinating rat brain.", "content": "Microsomal fractions of 14-day-old rat brain were incubated at pH 7.1 with 1-[1'-14C]-alk-1'-enyl-sn-glycero-3-phosphoethanolamine (lysoplasmalogen). 1-[1'-14C]alkenylglycerol was produced by hydrolyzing enzyme activities, which were stimulated by Mg2 and inhibited by SH-group reagents. Hydrolysis of 1-[1'-14C]alkyl-sn-glycero-3-phosphoethanolamine is very similar in this respect, but the Km value is higher in the former case. The 1-alkyl compound acts as a non-competitive inhibitor of the hydrolyzing enzyme activity described, whereas the hydrolysis of the 1-alkyl derivative is not inhibited by the 1-alkenyl compound.", "contents": "Studies on the hydrolysis of 1-alk-1'-enyl-sn-glycero-3-phosphoethanolamine by microsomes from myelinating rat brain. Microsomal fractions of 14-day-old rat brain were incubated at pH 7.1 with 1-[1'-14C]-alk-1'-enyl-sn-glycero-3-phosphoethanolamine (lysoplasmalogen). 1-[1'-14C]alkenylglycerol was produced by hydrolyzing enzyme activities, which were stimulated by Mg2 and inhibited by SH-group reagents. Hydrolysis of 1-[1'-14C]alkyl-sn-glycero-3-phosphoethanolamine is very similar in this respect, but the Km value is higher in the former case. The 1-alkyl compound acts as a non-competitive inhibitor of the hydrolyzing enzyme activity described, whereas the hydrolysis of the 1-alkyl derivative is not inhibited by the 1-alkenyl compound."}
{"id": "PMID:488921", "title": "Sclerosing stromal tumours of the ovary.", "content": "Sclerosing stromal tumours of the ovary have recently been described as a histologically and clinically distinct subgroup within the thecoma-fibroma spectrum of benign ovarian sex cord stromal tumours. Reported cases occurred predominantly in young women and only occasional tumours showed evidence of hormonal activity. The present series of five cases expands the spectra of both histological patterns and clinical presentations and suggests that the entity of sclerosing stromal tumours may not be as clearly circumscribed as has been previously reported.", "contents": "Sclerosing stromal tumours of the ovary. Sclerosing stromal tumours of the ovary have recently been described as a histologically and clinically distinct subgroup within the thecoma-fibroma spectrum of benign ovarian sex cord stromal tumours. Reported cases occurred predominantly in young women and only occasional tumours showed evidence of hormonal activity. The present series of five cases expands the spectra of both histological patterns and clinical presentations and suggests that the entity of sclerosing stromal tumours may not be as clearly circumscribed as has been previously reported."}
{"id": "PMID:488922", "title": "Prognostic significance of the histological features of malignant melanoma.", "content": "A review of 694 patients with localized cutaneous malignant melanoma (clinical stage I) revealed that three histological features of the primary lesion had no effect of their own on survival rate but derived their prognostic significance only because of their close correlation with tumour thickness. Primary lesions of superficial spreading histogenetic type, or of low mitotic activity or showing evidence of partial regression appeared to have a more favourable prognosis than lesions of nodular histogenetic type or of high mitotic activity or showing no regression. However, the former three histological features were predominant in thin lesions which had a better prognosis than thicker lesions. It was concluded that these features exerted only an indirect effect upon survival, tumour thickness being the most important prognostic determinant.", "contents": "Prognostic significance of the histological features of malignant melanoma. A review of 694 patients with localized cutaneous malignant melanoma (clinical stage I) revealed that three histological features of the primary lesion had no effect of their own on survival rate but derived their prognostic significance only because of their close correlation with tumour thickness. Primary lesions of superficial spreading histogenetic type, or of low mitotic activity or showing evidence of partial regression appeared to have a more favourable prognosis than lesions of nodular histogenetic type or of high mitotic activity or showing no regression. However, the former three histological features were predominant in thin lesions which had a better prognosis than thicker lesions. It was concluded that these features exerted only an indirect effect upon survival, tumour thickness being the most important prognostic determinant."}
{"id": "PMID:488923", "title": "Tubular carcinoma: a variant of secretory breast carcinoma.", "content": "Fifteen cases of tubular carcinoma of the breast have been studied using histochemical methods for mucosubstances, immunocytochemical methods for casein and actin and conventional electron microscopy. Mucosubstances and casein were demonstrated lying freely in the lumina of the tubules. Occasionally, mucosubstances and casein assumed the form of target-like intracytoplasmic 'inclusions' like those characteristically seen in lobular carcinoma. The neoplastic cells did not react with antisera specific against actin. Even at ultrastructural level no myoepithelial cells were observed, whilst villi were revealed along the tubular luminal surface. It appears that, in addition to distinctive biological, histological and ultrastructural features, tubular carcinoma has an almost constant histochemical pattern. This suggests a differentiation towards epithelial secretory cells engaged in intensive milk protein production which has also been shown to be a feature of lobular carcinoma. It is concluded that though lobular carcinoma and tubular carcinoma of the breast have been traditionally regarded as two distinct entities, they have certain similar functional characteristics and it is postulated that these two tumours could represent the extreme variants fo the same entity: the infiltrative lobular carcinoma being the most undifferentiated and tubular carcinoma the most highly differentiated.", "contents": "Tubular carcinoma: a variant of secretory breast carcinoma. Fifteen cases of tubular carcinoma of the breast have been studied using histochemical methods for mucosubstances, immunocytochemical methods for casein and actin and conventional electron microscopy. Mucosubstances and casein were demonstrated lying freely in the lumina of the tubules. Occasionally, mucosubstances and casein assumed the form of target-like intracytoplasmic 'inclusions' like those characteristically seen in lobular carcinoma. The neoplastic cells did not react with antisera specific against actin. Even at ultrastructural level no myoepithelial cells were observed, whilst villi were revealed along the tubular luminal surface. It appears that, in addition to distinctive biological, histological and ultrastructural features, tubular carcinoma has an almost constant histochemical pattern. This suggests a differentiation towards epithelial secretory cells engaged in intensive milk protein production which has also been shown to be a feature of lobular carcinoma. It is concluded that though lobular carcinoma and tubular carcinoma of the breast have been traditionally regarded as two distinct entities, they have certain similar functional characteristics and it is postulated that these two tumours could represent the extreme variants fo the same entity: the infiltrative lobular carcinoma being the most undifferentiated and tubular carcinoma the most highly differentiated."}
{"id": "PMID:488924", "title": "Myospherulosis: further ultrastructural observations.", "content": "The changes of myospherulosis were observed in masses from the buttocks of three patients. Ultrastructural examination of parent bodies and spherules from two patients revealed some spherules to contain dense bodies and filaments. Spherules had a double electron dense wall: the outer layer had a thickness corresponding to that of a cell membrane. Incubation of packed red blood cells with tetracycline ointment produced a similar electron dense deposit on the inner aspect of the cell membrane of the erythrocytes. No filaments or parent bodies were seen. Our findings support the hypothesis that myospherulosis represents altered red blood cells. The filaments seen in our patients may represent polymers of haemoglobin. It is suggested that parent bodies may be derived from histiocytes.", "contents": "Myospherulosis: further ultrastructural observations. The changes of myospherulosis were observed in masses from the buttocks of three patients. Ultrastructural examination of parent bodies and spherules from two patients revealed some spherules to contain dense bodies and filaments. Spherules had a double electron dense wall: the outer layer had a thickness corresponding to that of a cell membrane. Incubation of packed red blood cells with tetracycline ointment produced a similar electron dense deposit on the inner aspect of the cell membrane of the erythrocytes. No filaments or parent bodies were seen. Our findings support the hypothesis that myospherulosis represents altered red blood cells. The filaments seen in our patients may represent polymers of haemoglobin. It is suggested that parent bodies may be derived from histiocytes."}
{"id": "PMID:488929", "title": "Psychiatric manpower: an overview.", "content": "There are approximately 25,000 to 30,000 psychiatrists in the United States, some 17,000 of whom are in actual clinical practice. As part of an overview of psychiatric manpower, the authors show the distribution of psychiatrists by state and present population-per-psychiatrist ratios. In discussing the distribution of psychiatrists in various work settings, they note that the decreasing percentages of psychiatrists in community mental health centers may be related to such factors as the large number of non-hospital-based centers, growing antimedical attitudes in centers, and psychiatrists' inclination to work in a setting similar to their training site. They believe that federal and state support should be increased for university-affiliated psychiatric training programs based in settings where psychiatrists are needed: state hospitals, VA hospitals, community mental health centers, and similar facilities. Such an approach would result in the recruitment and retention of greater numbers of psychiatrists in public service settings.", "contents": "Psychiatric manpower: an overview. There are approximately 25,000 to 30,000 psychiatrists in the United States, some 17,000 of whom are in actual clinical practice. As part of an overview of psychiatric manpower, the authors show the distribution of psychiatrists by state and present population-per-psychiatrist ratios. In discussing the distribution of psychiatrists in various work settings, they note that the decreasing percentages of psychiatrists in community mental health centers may be related to such factors as the large number of non-hospital-based centers, growing antimedical attitudes in centers, and psychiatrists' inclination to work in a setting similar to their training site. They believe that federal and state support should be increased for university-affiliated psychiatric training programs based in settings where psychiatrists are needed: state hospitals, VA hospitals, community mental health centers, and similar facilities. Such an approach would result in the recruitment and retention of greater numbers of psychiatrists in public service settings."}
{"id": "PMID:488930", "title": "Psychiatry in public service: challenge of the eighties.", "content": "Contrary to popular opinion, there are now more psychiatrists in public service than ever before. Psychiatrists' contributions in this area have been masked by a decreasing growth in the total number of psychiatrists, the simultaneous expansion of other mental health disciplines, and the proliferation of mental health treatment facilities. The authors examine the reasons for the decline in the over-all growth of the profession and describe some new federal initiatives to encourage more American medical graduates to specialize in psychiatry and to work in traditionally underserved areas in the public sector.", "contents": "Psychiatry in public service: challenge of the eighties. Contrary to popular opinion, there are now more psychiatrists in public service than ever before. Psychiatrists' contributions in this area have been masked by a decreasing growth in the total number of psychiatrists, the simultaneous expansion of other mental health disciplines, and the proliferation of mental health treatment facilities. The authors examine the reasons for the decline in the over-all growth of the profession and describe some new federal initiatives to encourage more American medical graduates to specialize in psychiatry and to work in traditionally underserved areas in the public sector."}
{"id": "PMID:488931", "title": "Special problems facing the psychiatrist-administrator.", "content": "Psychiatrists who become administrators often find themselves in an unfamiliar world of planning, budget and cost control, labor relations, and mental health law. They discover that the physical and mental demands of leadership are great, their role makes them highly visible, and their future is uncertain. Psychiatric administrators in general hospitals are in a unique position of competing for resources with medicine and surgery. In addition, the need for linkages to the community challenges the psychiatric administrator to deliver services to defined catchment areas, identify cases at risk, and establish halfway houses and similar facilities. While the author discusses primarily the stresses and problems of being a psychiatric administrator, he also says there are special satisfactions of the executive role.", "contents": "Special problems facing the psychiatrist-administrator. Psychiatrists who become administrators often find themselves in an unfamiliar world of planning, budget and cost control, labor relations, and mental health law. They discover that the physical and mental demands of leadership are great, their role makes them highly visible, and their future is uncertain. Psychiatric administrators in general hospitals are in a unique position of competing for resources with medicine and surgery. In addition, the need for linkages to the community challenges the psychiatric administrator to deliver services to defined catchment areas, identify cases at risk, and establish halfway houses and similar facilities. While the author discusses primarily the stresses and problems of being a psychiatric administrator, he also says there are special satisfactions of the executive role."}
{"id": "PMID:488932", "title": "Toward increased psychiatric presence in community mental health centers.", "content": "The presence of psychiatrists in community mental health centers has diminished in recent years, especially in centers that are non-hospital-based and that are located in rural or disadvantaged urban settings. The decrease in psychiatric leadership in the centers is particularly notable. Factors contributing to the trend include lower salaries than in the private sector, a decrease in the number of patients with severe mental disorders coming to the centers, and the impact of a decrease in specialized training programs in community psychiatry. The authors suggest several incentives to increase psychiatric presence in the centers, including developing staffing standards as a condition of funding, giving psychiatrists time to do research and evaluation and to teach, and increasing the medical involvement of centers through links with general hospitals, private psychiatric hospitals, and medical schools.", "contents": "Toward increased psychiatric presence in community mental health centers. The presence of psychiatrists in community mental health centers has diminished in recent years, especially in centers that are non-hospital-based and that are located in rural or disadvantaged urban settings. The decrease in psychiatric leadership in the centers is particularly notable. Factors contributing to the trend include lower salaries than in the private sector, a decrease in the number of patients with severe mental disorders coming to the centers, and the impact of a decrease in specialized training programs in community psychiatry. The authors suggest several incentives to increase psychiatric presence in the centers, including developing staffing standards as a condition of funding, giving psychiatrists time to do research and evaluation and to teach, and increasing the medical involvement of centers through links with general hospitals, private psychiatric hospitals, and medical schools."}
{"id": "PMID:488933", "title": "Public psychiatry: the public's view.", "content": "A 1977 survey of public attitudes toward psychiatry found that the quality of institutional psychiatry was one of several factors associated with negative attitudes toward the field. Noting the shortage of psychiatrists in public institutions, the author questions whether the public associates psychiatrists with conditions in the institutions despite their lack of involvement, or because of it. He believes that the inadequate care provided in many public facilities is a result of a tacit social contract between the public and private sectors that enables private institutions to maintain their fiscal integrity and their quality of care by moving undesirable patients to public institutions that provide inadequate care. Although the problems with the system extend beyond psychiatry and the mental health field, he suggests that psychiatrists could force some positive changes by refusing as a profession to practice in institutions that provide inadequate care, and by supporting the delivery of public services by nongovernmental bodies. The ultimate answer, he believes, lies in research into more effective treatment for mental illness that would make such institutions unnecessary.", "contents": "Public psychiatry: the public's view. A 1977 survey of public attitudes toward psychiatry found that the quality of institutional psychiatry was one of several factors associated with negative attitudes toward the field. Noting the shortage of psychiatrists in public institutions, the author questions whether the public associates psychiatrists with conditions in the institutions despite their lack of involvement, or because of it. He believes that the inadequate care provided in many public facilities is a result of a tacit social contract between the public and private sectors that enables private institutions to maintain their fiscal integrity and their quality of care by moving undesirable patients to public institutions that provide inadequate care. Although the problems with the system extend beyond psychiatry and the mental health field, he suggests that psychiatrists could force some positive changes by refusing as a profession to practice in institutions that provide inadequate care, and by supporting the delivery of public services by nongovernmental bodies. The ultimate answer, he believes, lies in research into more effective treatment for mental illness that would make such institutions unnecessary."}
{"id": "PMID:488934", "title": "Distribution of mental health professionals in the South: a research project.", "content": "The Southern Regional Education Board is carrying out a project to determine what factors influence the distribution of mental health professionals in the South and to develop recommendations for improving distribution. The project is concerned with four major aspects of distribution: alleviating geographic maldistribution, increasing the number of professionals in specific subspecialties, providing more professionals for service in the public sector, and training more minority professionals. Staff of training programs, trainees, graduates, and staff of urban and rural community mental health centers and state hospitals are being surveyed, through interviews and questionnaires, to try to determine what factors influence professionals' decisions about where to locate and what kind of practice to enter.", "contents": "Distribution of mental health professionals in the South: a research project. The Southern Regional Education Board is carrying out a project to determine what factors influence the distribution of mental health professionals in the South and to develop recommendations for improving distribution. The project is concerned with four major aspects of distribution: alleviating geographic maldistribution, increasing the number of professionals in specific subspecialties, providing more professionals for service in the public sector, and training more minority professionals. Staff of training programs, trainees, graduates, and staff of urban and rural community mental health centers and state hospitals are being surveyed, through interviews and questionnaires, to try to determine what factors influence professionals' decisions about where to locate and what kind of practice to enter."}
{"id": "PMID:488935", "title": "A state perspective on psychiatric manpower development.", "content": "When the shortage of qualified psychiatrists and other physicians in Ohio's mental institutions reached the critical point in 1975, a number of measures were implemented to upgrade medical manpower. Salaries were increased, and the leadership of the medical and psychiatric community was enlisted to help encourage physicians to enter public service. In addition, clinical training programs were shifted from the traditional freestanding state-hospital-based programs to programs operated by medical colleges. The author reports that the state has made steady progress in reducing psychiatric manpower shortages, but he notes that new trends, such as the diminishing interest of American medical students in psychiatry, threaten to erode the gains.", "contents": "A state perspective on psychiatric manpower development. When the shortage of qualified psychiatrists and other physicians in Ohio's mental institutions reached the critical point in 1975, a number of measures were implemented to upgrade medical manpower. Salaries were increased, and the leadership of the medical and psychiatric community was enlisted to help encourage physicians to enter public service. In addition, clinical training programs were shifted from the traditional freestanding state-hospital-based programs to programs operated by medical colleges. The author reports that the state has made steady progress in reducing psychiatric manpower shortages, but he notes that new trends, such as the diminishing interest of American medical students in psychiatry, threaten to erode the gains."}
{"id": "PMID:488936", "title": "Why psychiatrists leave the public sector.", "content": "The flight of psychiatrists from public mental health facilities must be halted if the sickest psychiatric patients--the severely and chronically mentally ill--are to receive the best care and treatment possible. The author emphasizes the need for commitment by organized psychiatry, universities, and communities to support the public sector and those working in it. He examines the factors that influence psychiatrists to enter public service and those that eventually cause them to leave. He notes that the departure of public hospital psychiatrists for quasi-public settings has parallelled the transfer of patients to community settings, and that these psychiatrists may now be treating in such settings patients they once saw in the hospital.", "contents": "Why psychiatrists leave the public sector. The flight of psychiatrists from public mental health facilities must be halted if the sickest psychiatric patients--the severely and chronically mentally ill--are to receive the best care and treatment possible. The author emphasizes the need for commitment by organized psychiatry, universities, and communities to support the public sector and those working in it. He examines the factors that influence psychiatrists to enter public service and those that eventually cause them to leave. He notes that the departure of public hospital psychiatrists for quasi-public settings has parallelled the transfer of patients to community settings, and that these psychiatrists may now be treating in such settings patients they once saw in the hospital."}
{"id": "PMID:488937", "title": "Psychiatry's human resources: 20 years later.", "content": "The author compares the findings he made about the shortage of psychiatrists 20 years ago for the Joint Commission on Mental Illness and Health with the recent findings of the President's Commission on Mental Health. He concludes that not much has changed except for an increased awareness today of the need for services for minorities, and heightened concern over the low numbers of minorities in professional positions and training programs. The shortage of psychiatrists to work with poor people, children and adolescents, the aged, the organically impaired, and the physically handicapped continues, and it is unlikely to be overcome because of the drop in the number of American medical school graduates entering psychiatric training and the federal law restricting the number of foreign medical graduates entering the United States. He believes primary prevention is the best way to ease the widening gap between the need for services and the available psychiatric resources.", "contents": "Psychiatry's human resources: 20 years later. The author compares the findings he made about the shortage of psychiatrists 20 years ago for the Joint Commission on Mental Illness and Health with the recent findings of the President's Commission on Mental Health. He concludes that not much has changed except for an increased awareness today of the need for services for minorities, and heightened concern over the low numbers of minorities in professional positions and training programs. The shortage of psychiatrists to work with poor people, children and adolescents, the aged, the organically impaired, and the physically handicapped continues, and it is unlikely to be overcome because of the drop in the number of American medical school graduates entering psychiatric training and the federal law restricting the number of foreign medical graduates entering the United States. He believes primary prevention is the best way to ease the widening gap between the need for services and the available psychiatric resources."}
{"id": "PMID:488942", "title": "Hospitals must be businesslike. Interview by Alan J. Appelbaum.", "content": "AHA's new chairman-elect designate believes that today's not-for-profit hospital is a business enterprise and that it might benefit by following a corporate model.", "contents": "Hospitals must be businesslike. Interview by Alan J. Appelbaum. AHA's new chairman-elect designate believes that today's not-for-profit hospital is a business enterprise and that it might benefit by following a corporate model."}
{"id": "PMID:488943", "title": "Less is more in rural hospital staffing.", "content": "The concept of multidisciplinary staff members grew out of the manpower needs and financial limitations of rural hospitals, but the idea is now sparking new interest.", "contents": "Less is more in rural hospital staffing. The concept of multidisciplinary staff members grew out of the manpower needs and financial limitations of rural hospitals, but the idea is now sparking new interest."}
{"id": "PMID:488944", "title": "This is the way it was in the 1970s.", "content": "Although regulatory, economic, and other concerns appear to have taken center stage in the health care field in the past decade, the real work of healing and comforting the sick goes on.", "contents": "This is the way it was in the 1970s. Although regulatory, economic, and other concerns appear to have taken center stage in the health care field in the past decade, the real work of healing and comforting the sick goes on."}
{"id": "PMID:488945", "title": "Medical care on a day in May.", "content": "Each May, the Indianapolis Motor Speedway medical department sets up a temporary emergency facility that is probably the most complete of its kind in the world.", "contents": "Medical care on a day in May. Each May, the Indianapolis Motor Speedway medical department sets up a temporary emergency facility that is probably the most complete of its kind in the world."}
{"id": "PMID:488946", "title": "Food buying undergoes great changes.", "content": "The shrewd buyer has been replaced by a systems-oriented buyer who is part of a changed food procurement function, which includes shared services and quality assurance programs.", "contents": "Food buying undergoes great changes. The shrewd buyer has been replaced by a systems-oriented buyer who is part of a changed food procurement function, which includes shared services and quality assurance programs."}
{"id": "PMID:488947", "title": "System for waste disposal reclaims heat.", "content": "One hospital's search for a new incinerator that would meet antipollution regulations led to selection and installation of a system that also conserves energy through heat reclamation.", "contents": "System for waste disposal reclaims heat. One hospital's search for a new incinerator that would meet antipollution regulations led to selection and installation of a system that also conserves energy through heat reclamation."}
{"id": "PMID:488955", "title": "Patients, not unions, first concern of hospitals.", "content": "Although the law regarding employee organizational rights in health care institutions has been in a state of flux since 1974, it is now clear that patient care generally takes precedence over employees' rights to organize on hospital premises.", "contents": "Patients, not unions, first concern of hospitals. Although the law regarding employee organizational rights in health care institutions has been in a state of flux since 1974, it is now clear that patient care generally takes precedence over employees' rights to organize on hospital premises."}
{"id": "PMID:488956", "title": "Multihospital system enhances employee relations, programs.", "content": "Key elements of a successful personnel management program created within a new multihospital system are presented, along with the difficulties and opportunities accompanying the changeover.", "contents": "Multihospital system enhances employee relations, programs. Key elements of a successful personnel management program created within a new multihospital system are presented, along with the difficulties and opportunities accompanying the changeover."}
{"id": "PMID:488957", "title": "Quality control enhanced by chart review.", "content": "A long-term care facility developed, tested, and implemented criteria for documenting care, a procedure for regular review of charts, and quantitative methods of evaluating reviews.", "contents": "Quality control enhanced by chart review. A long-term care facility developed, tested, and implemented criteria for documenting care, a procedure for regular review of charts, and quantitative methods of evaluating reviews."}
{"id": "PMID:488958", "title": "Forum sparks creative energy.", "content": "Human support committee provides a forum where staff, patients, and administration can become involved in the planning, implementation, and evaluation of patient services.", "contents": "Forum sparks creative energy. Human support committee provides a forum where staff, patients, and administration can become involved in the planning, implementation, and evaluation of patient services."}
{"id": "PMID:488959", "title": "Medical center supports patient advocacy.", "content": "At Hurley Medical Center, patient advocates enjoy an amiable relationship with administrators and are considered a valuable management information resource.", "contents": "Medical center supports patient advocacy. At Hurley Medical Center, patient advocates enjoy an amiable relationship with administrators and are considered a valuable management information resource."}
{"id": "PMID:488960", "title": "Comfort, care for families who must wait.", "content": "Volunteers staff a special waiting room that extends humanistic care to meet the needs of families as they wait to learn the outcomes of their relatives' surgery or tests.", "contents": "Comfort, care for families who must wait. Volunteers staff a special waiting room that extends humanistic care to meet the needs of families as they wait to learn the outcomes of their relatives' surgery or tests."}
{"id": "PMID:488972", "title": "Prospects for humane care are hopeful.", "content": "Token deference to humanism will not get the job done/The goal of humanizing care must be given high priority and be made consistent with other hospital objectives.", "contents": "Prospects for humane care are hopeful. Token deference to humanism will not get the job done/The goal of humanizing care must be given high priority and be made consistent with other hospital objectives."}
{"id": "PMID:488973", "title": "Can hospitals afford to care less?", "content": "\"Humanistic health care\" has a pleasant ring to it, but hospital employees, administrators, physicians, and trustees must support such an environment for it to have any real value.", "contents": "Can hospitals afford to care less? \"Humanistic health care\" has a pleasant ring to it, but hospital employees, administrators, physicians, and trustees must support such an environment for it to have any real value."}
{"id": "PMID:488974", "title": "Hospitals need humanism.", "content": "Just as technology is growing rapidly and is being carefully \"mapped out,\" health care providers also must \"map out\" the most humane way of delivering health care.", "contents": "Hospitals need humanism. Just as technology is growing rapidly and is being carefully \"mapped out,\" health care providers also must \"map out\" the most humane way of delivering health care."}
{"id": "PMID:488975", "title": "Burnout: job hazard for health workers.", "content": "Employees in health care settings face significant risk of burnout, which involves negative emotional, psychological, and physical reactions to continuous work-related stress.", "contents": "Burnout: job hazard for health workers. Employees in health care settings face significant risk of burnout, which involves negative emotional, psychological, and physical reactions to continuous work-related stress."}
{"id": "PMID:488976", "title": "Staff skills in patient/family relations grow.", "content": "Short but cogent course on communication, attitudes, and behaviors helps staff members from many departments relate more effectively with patients, their families, and visitors.", "contents": "Staff skills in patient/family relations grow. Short but cogent course on communication, attitudes, and behaviors helps staff members from many departments relate more effectively with patients, their families, and visitors."}
{"id": "PMID:488984", "title": "Defining hospital market efficiency--the community's task.", "content": "Hospitals must concentrate on both market and productive efficiency to control costs and satisfy customers' health care needs. Local communities can set market efficiency standards using data sets similar to the Michigan study's.", "contents": "Defining hospital market efficiency--the community's task. Hospitals must concentrate on both market and productive efficiency to control costs and satisfy customers' health care needs. Local communities can set market efficiency standards using data sets similar to the Michigan study's."}
{"id": "PMID:488985", "title": "Selecting leaders through shared decision making.", "content": "The WEVAS procedure, through use of consultative decision making, facilitates the selection of CEOs who will be accepted by associates and subordinates and thus will be able to function with maximum effectiveness.", "contents": "Selecting leaders through shared decision making. The WEVAS procedure, through use of consultative decision making, facilitates the selection of CEOs who will be accepted by associates and subordinates and thus will be able to function with maximum effectiveness."}
{"id": "PMID:488986", "title": "Choosing a CEO: the necessity of shared Christian values.", "content": "The CEO candidate and the Catholic health care facility must clarify expectations and determine if the candidate's values can be expressed administratively to mesh with the organization's philosophy.", "contents": "Choosing a CEO: the necessity of shared Christian values. The CEO candidate and the Catholic health care facility must clarify expectations and determine if the candidate's values can be expressed administratively to mesh with the organization's philosophy."}
{"id": "PMID:488987", "title": "The Canon Law revisions: a reflection of the postconciliar Church?", "content": "The postconciliar Church has approved many changes in Church life. Reactions to the proposed draft of the new canon law codes have ranged from favorable to strongly critical. The mature Catholic looks beyond the letter of the law to its spirit and purpose.", "contents": "The Canon Law revisions: a reflection of the postconciliar Church? The postconciliar Church has approved many changes in Church life. Reactions to the proposed draft of the new canon law codes have ranged from favorable to strongly critical. The mature Catholic looks beyond the letter of the law to its spirit and purpose."}
{"id": "PMID:488989", "title": "Needed: model law on the charitable corporation.", "content": "Nearly a dozen years ago, the McGrath Thesis stimulated controversy about Catholic corporations as property owners. Though no court has ever ruled that a charitable corporation's control is vested in the general public, institutions should understand state and federal laws that affect both property ownership and its dissolution.", "contents": "Needed: model law on the charitable corporation. Nearly a dozen years ago, the McGrath Thesis stimulated controversy about Catholic corporations as property owners. Though no court has ever ruled that a charitable corporation's control is vested in the general public, institutions should understand state and federal laws that affect both property ownership and its dissolution."}
{"id": "PMID:489004", "title": "Another elliptocytosis locus on chromosome 1?", "content": "Analysis of pedigrees given in the literature suggests that a second elliptocytosis locus (not linked to Rh) may be linked to Duffy (Z = 1.97 at theta = 0.0), and therefore on chromosome 1. Significant heterogeneity is found between the El1 and El2 total lod scores with Fy.", "contents": "Another elliptocytosis locus on chromosome 1? Analysis of pedigrees given in the literature suggests that a second elliptocytosis locus (not linked to Rh) may be linked to Duffy (Z = 1.97 at theta = 0.0), and therefore on chromosome 1. Significant heterogeneity is found between the El1 and El2 total lod scores with Fy."}
{"id": "PMID:489005", "title": "A ring chromosome 9 in an infant with malformations.", "content": "A low-birth-weight infant with malformed upper extremities and congenital heart disease was observed. Cytogenetic analysis revealed a 46,XY,r(9)(p24q34) complement.", "contents": "A ring chromosome 9 in an infant with malformations. A low-birth-weight infant with malformed upper extremities and congenital heart disease was observed. Cytogenetic analysis revealed a 46,XY,r(9)(p24q34) complement."}
{"id": "PMID:489006", "title": "Trisomy 9p with i(9p) and t(9q18p).", "content": "The trisomy 9p syndrome in a 2-year-old girl with moderate mental retardation is presented. She has a unique karyotype with a de novo isochromosome 9p and a translocation between 9q and 18p.", "contents": "Trisomy 9p with i(9p) and t(9q18p). The trisomy 9p syndrome in a 2-year-old girl with moderate mental retardation is presented. She has a unique karyotype with a de novo isochromosome 9p and a translocation between 9q and 18p."}
{"id": "PMID:489007", "title": "Partial trisomy of 13(pter to q12) due to 47,XY, + der(13),t(13;22)(q12;q13)mat.", "content": "A 36-month-old boy presented with short stature, short neck, shield-shaped chest, and mental retardation. Chromosome analysis showed trisomy for the short arm and the proximal protion of the long arm of chromosome 13 [47,XY, + der(13),t(13;22)(q12;q13)mat]. The patient's mother has a balanced translocation between the long arms of chromosomes 13 and 22 [46,XX,t(13;22)(q12;q13)]. The patient's neutrophils showed an elevated number of nuclear projections and his fetal hemoglobin level was undetectable.", "contents": "Partial trisomy of 13(pter to q12) due to 47,XY, + der(13),t(13;22)(q12;q13)mat. A 36-month-old boy presented with short stature, short neck, shield-shaped chest, and mental retardation. Chromosome analysis showed trisomy for the short arm and the proximal protion of the long arm of chromosome 13 [47,XY, + der(13),t(13;22)(q12;q13)mat]. The patient's mother has a balanced translocation between the long arms of chromosomes 13 and 22 [46,XX,t(13;22)(q12;q13)]. The patient's neutrophils showed an elevated number of nuclear projections and his fetal hemoglobin level was undetectable."}
{"id": "PMID:489008", "title": "X-linked mental retardation with macro-orchidism and marker X chromosomes.", "content": "A family with X-linked mental retardation and a marker X chromosome was ascertained by the presence of macro-orchidism in the three institutionalized probands. Verbal evaluation revealed a generalized language disability with commonly occurring articulation errors. The heterozygous females in this family exhibited some reduction in mental ability; the marker X chromosome was demonstrated in both sexes.", "contents": "X-linked mental retardation with macro-orchidism and marker X chromosomes. A family with X-linked mental retardation and a marker X chromosome was ascertained by the presence of macro-orchidism in the three institutionalized probands. Verbal evaluation revealed a generalized language disability with commonly occurring articulation errors. The heterozygous females in this family exhibited some reduction in mental ability; the marker X chromosome was demonstrated in both sexes."}
{"id": "PMID:489009", "title": "Familial translocation t(10;21)(q22;q22).", "content": "A family is described with a translocation t(10;21)(q22;q22) transmitted through three generations. This family was studied for the apparition of several miscarriages and two sisters with multiple malformations. Both children had a probably partial trisomy of chromosome 10 and a monosomy of chromosome 21 due to a maternal adjacent-2 meiotic segregation.", "contents": "Familial translocation t(10;21)(q22;q22). A family is described with a translocation t(10;21)(q22;q22) transmitted through three generations. This family was studied for the apparition of several miscarriages and two sisters with multiple malformations. Both children had a probably partial trisomy of chromosome 10 and a monosomy of chromosome 21 due to a maternal adjacent-2 meiotic segregation."}
{"id": "PMID:489010", "title": "Cytogenetic and clinical studies in five cases of inv dup(15).", "content": "Inv dup(15) is a clinically significant bisatellited derivative of chromosome 15. Five unrelated patients with this abnormality are described and compared with ten confirmed and nine suspected cases in the literature. Mental and developmental retardation, hypotonia, behavioral disturbances, seizures, abnormal dermatoglyphics, and mild somatic anomalies were the most consistent findings. The extra chromosomes in our patients were identified with the aid of various techniques, including distamycin A/DAPI banding. A comparison of satellite polymorphisms suggested that the rearrangements frequently arose by meiotic nonsister chromatid exchange and second-division nondisjunction. A maternal origin was indicated in two cases, and parental ages were distinctly elevated.", "contents": "Cytogenetic and clinical studies in five cases of inv dup(15). Inv dup(15) is a clinically significant bisatellited derivative of chromosome 15. Five unrelated patients with this abnormality are described and compared with ten confirmed and nine suspected cases in the literature. Mental and developmental retardation, hypotonia, behavioral disturbances, seizures, abnormal dermatoglyphics, and mild somatic anomalies were the most consistent findings. The extra chromosomes in our patients were identified with the aid of various techniques, including distamycin A/DAPI banding. A comparison of satellite polymorphisms suggested that the rearrangements frequently arose by meiotic nonsister chromatid exchange and second-division nondisjunction. A maternal origin was indicated in two cases, and parental ages were distinctly elevated."}
{"id": "PMID:489011", "title": "Concordant congenital malformations in twins with inherited translocation: t(9p--;13q+).", "content": "Several members of a family with a translocation between the short arm of chromosome 9 and the long arm of chromosome 13(9p--;13q+) are presented. Although the translocation found in various members of the family looked alike and appeared to be balanced, the clinical features were different. The like-sex twins displayed some features of 9p monosomy syndrome, whereas their mother and maternal grandmother, who apparently had the same translocation, showed only a few features of 9p-- syndrome in addition to mild mental retardation. We suggest that a minute deletion of the short arm of chromosome 9 may cause features of 9p-- syndrome and that the clinical features of this syndrome in older individuals may be too mild for the clinical diagnosis to be possible.", "contents": "Concordant congenital malformations in twins with inherited translocation: t(9p--;13q+). Several members of a family with a translocation between the short arm of chromosome 9 and the long arm of chromosome 13(9p--;13q+) are presented. Although the translocation found in various members of the family looked alike and appeared to be balanced, the clinical features were different. The like-sex twins displayed some features of 9p monosomy syndrome, whereas their mother and maternal grandmother, who apparently had the same translocation, showed only a few features of 9p-- syndrome in addition to mild mental retardation. We suggest that a minute deletion of the short arm of chromosome 9 may cause features of 9p-- syndrome and that the clinical features of this syndrome in older individuals may be too mild for the clinical diagnosis to be possible."}
{"id": "PMID:489012", "title": "The influence of culture medium composition on the incidence of chromosomal breakage.", "content": "In patients with chromosomal instability and healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC medium 199, Eagle's Minimum Essential Medium, or RPMI, or RPMI 1629. The importance of the choice of culture medium for mutagenicity testing and studies of so-called spontaneous breakage is shown. Cultures incubated with TC Medium 199 showed the highest chromosomal breakage incidence.", "contents": "The influence of culture medium composition on the incidence of chromosomal breakage. In patients with chromosomal instability and healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC medium 199, Eagle's Minimum Essential Medium, or RPMI, or RPMI 1629. The importance of the choice of culture medium for mutagenicity testing and studies of so-called spontaneous breakage is shown. Cultures incubated with TC Medium 199 showed the highest chromosomal breakage incidence."}
{"id": "PMID:489013", "title": "Terminal 7p deletion and 1;7 translocation associated with craniosynostosis.", "content": "A female infant with presumptive deletion of the 7p2 region and an unusual translocation between a part of the short arm of chromosome 1 and a deleted chromosome 7 is described. The patient showed congenital craniosynostosis of the coronal and metopic sutures; marked turricephaly; hypotelorism; deeply cleft palate; shallow orbits with prominent bulging eyes; a depressed nasal bridge; anteverted nostrils; short hands with broad thin fingers and elongated thumbs; a mild talipes calcaneovalgus deformity of the feet; a systolic murmur due to a small VSD; and psychomotor retardation. The child died of bronchopneumonia at 10 weeks of age. The parents are chromosomally normal.", "contents": "Terminal 7p deletion and 1;7 translocation associated with craniosynostosis. A female infant with presumptive deletion of the 7p2 region and an unusual translocation between a part of the short arm of chromosome 1 and a deleted chromosome 7 is described. The patient showed congenital craniosynostosis of the coronal and metopic sutures; marked turricephaly; hypotelorism; deeply cleft palate; shallow orbits with prominent bulging eyes; a depressed nasal bridge; anteverted nostrils; short hands with broad thin fingers and elongated thumbs; a mild talipes calcaneovalgus deformity of the feet; a systolic murmur due to a small VSD; and psychomotor retardation. The child died of bronchopneumonia at 10 weeks of age. The parents are chromosomally normal."}
{"id": "PMID:489014", "title": "Sister chromatid exchange in peripheral lymphocytes of subjects vaccinated against measles.", "content": "The SCE frequency was studied in cultures of peripheral lymphocytes from three subjects before and after vaccination against measles. The immunological vaccination reactions were monitored by antibody titration and by measurement of DNA synthesis in peripheral lymphocytes. In two of the subjects, on the 14th day after vaccination, there was a marked decrease of the SCE frequency coinciding with common clinical vaccination reactions and an increase of DNA synthesis in the peripheral lymphocytes. The increase of antibody titers started on the 17th day. One month later, when the immunological reactions had subsided, the SCE frequency was increased by 25% over the prevaccination level. Third subject displayed a delayed vaccination response due to a simultaneous influenza infection. This subject showed a 50% increase in the SCE frequency on the 14th day as well as 6 weeks after vaccination. These results suggest that significant changes in the SCE frequency may be related to immunological vaccination reactions.", "contents": "Sister chromatid exchange in peripheral lymphocytes of subjects vaccinated against measles. The SCE frequency was studied in cultures of peripheral lymphocytes from three subjects before and after vaccination against measles. The immunological vaccination reactions were monitored by antibody titration and by measurement of DNA synthesis in peripheral lymphocytes. In two of the subjects, on the 14th day after vaccination, there was a marked decrease of the SCE frequency coinciding with common clinical vaccination reactions and an increase of DNA synthesis in the peripheral lymphocytes. The increase of antibody titers started on the 17th day. One month later, when the immunological reactions had subsided, the SCE frequency was increased by 25% over the prevaccination level. Third subject displayed a delayed vaccination response due to a simultaneous influenza infection. This subject showed a 50% increase in the SCE frequency on the 14th day as well as 6 weeks after vaccination. These results suggest that significant changes in the SCE frequency may be related to immunological vaccination reactions."}
{"id": "PMID:489015", "title": "Cycloheximide-resistance in Chinese hamster ovary cells and human fibroblast cells. Cytogenetic and biochemical characterization.", "content": "Cycloheximide(CHM)-resistant mutant Chinese hamster ovary (CHO) and human cells were induced with N-nitrosomethylurea (NMU) and ethyl methanesulfonate (EMS); the mutants were viable and showed unlimited growth in the presence of CHM (7 X 10(-7) M), whereas this concentration inhibits protein synthesis in vivo as well as in vitro. No numerical or structural chromosomal aberrations were found in the mutant cells. In vitro analysis shows that the ribosomes confer resistance against cycloheximide.", "contents": "Cycloheximide-resistance in Chinese hamster ovary cells and human fibroblast cells. Cytogenetic and biochemical characterization. Cycloheximide(CHM)-resistant mutant Chinese hamster ovary (CHO) and human cells were induced with N-nitrosomethylurea (NMU) and ethyl methanesulfonate (EMS); the mutants were viable and showed unlimited growth in the presence of CHM (7 X 10(-7) M), whereas this concentration inhibits protein synthesis in vivo as well as in vitro. No numerical or structural chromosomal aberrations were found in the mutant cells. In vitro analysis shows that the ribosomes confer resistance against cycloheximide."}
{"id": "PMID:489016", "title": "Determinants of plasma uric acid.", "content": "Obesity, alcohol consumption, and hematocrit provide an index of plasma uric acid, which in path analysis has a cultural heritability of 0.11 in children and 0.23 in parents, a small maternal effect, and a genetic heritability of 0.25 in both generations. Preliminary evidence for a major locus is destroyed by the omission of one exceptional child. There is no evidence against the polygenic hypothesis for hyperuricemia in the Japanese-American population studied.", "contents": "Determinants of plasma uric acid. Obesity, alcohol consumption, and hematocrit provide an index of plasma uric acid, which in path analysis has a cultural heritability of 0.11 in children and 0.23 in parents, a small maternal effect, and a genetic heritability of 0.25 in both generations. Preliminary evidence for a major locus is destroyed by the omission of one exceptional child. There is no evidence against the polygenic hypothesis for hyperuricemia in the Japanese-American population studied."}
{"id": "PMID:489024", "title": "Association between Duffy blood groups and serum level of the pregnancy zone protein.", "content": "In a previous study of psoriatic patients we found increased serum levels of the pregnancy zone protein (PZ) in individuals of blood groups O and Fy(a-). In this study we found an association between the PZ serum level and the Fy(a-) blood group also in young healthy males. Thus the association between the PZ serum level and Duffy blood groups appears to apply to both normal and pathological conditions.", "contents": "Association between Duffy blood groups and serum level of the pregnancy zone protein. In a previous study of psoriatic patients we found increased serum levels of the pregnancy zone protein (PZ) in individuals of blood groups O and Fy(a-). In this study we found an association between the PZ serum level and the Fy(a-) blood group also in young healthy males. Thus the association between the PZ serum level and Duffy blood groups appears to apply to both normal and pathological conditions."}
{"id": "PMID:489025", "title": "Red cell glyoxalase I polymorphism in Italians. Report of a variant phenotype.", "content": "The glyoxalase I polymorphism was studied in 1,490 unrelated subjects from three different areas of Italy (Milan, Rome and Naples). The following frequencies of GLO2 allele were observed: Milan 0.57, Rome 0.59, Naples 0.61, and a significant difference between Milan and Naples was found (p less than 0.02). An anomalous phenotype is also described which permits us to postulate a new allele producing an isozyme with low activity and electrophoretically slower than those corresponding to GLO1 and GLO2 alleles.", "contents": "Red cell glyoxalase I polymorphism in Italians. Report of a variant phenotype. The glyoxalase I polymorphism was studied in 1,490 unrelated subjects from three different areas of Italy (Milan, Rome and Naples). The following frequencies of GLO2 allele were observed: Milan 0.57, Rome 0.59, Naples 0.61, and a significant difference between Milan and Naples was found (p less than 0.02). An anomalous phenotype is also described which permits us to postulate a new allele producing an isozyme with low activity and electrophoretically slower than those corresponding to GLO1 and GLO2 alleles."}
{"id": "PMID:489026", "title": "Human red cell glyoxalase I polymorphism in Denmark and its application to paternity cases.", "content": "Phenotypes of glyoxalase I (GLO) were determined in 1220 unrelated adults from all parts of Denmark giving the gene frequencies GLO1 = 0.4311 and GLO2 = 0.5689. The segregation of phenotypes in 59 families and in 455 mother-child pairs was consistent with the assumed autosomal codominant inheritance. The results of an investigation of 379 parternity cases with respect to exclusions of non-fathers by means of the GLO system are reported, and the application of the GLO system to paternity cases is discussed.", "contents": "Human red cell glyoxalase I polymorphism in Denmark and its application to paternity cases. Phenotypes of glyoxalase I (GLO) were determined in 1220 unrelated adults from all parts of Denmark giving the gene frequencies GLO1 = 0.4311 and GLO2 = 0.5689. The segregation of phenotypes in 59 families and in 455 mother-child pairs was consistent with the assumed autosomal codominant inheritance. The results of an investigation of 379 parternity cases with respect to exclusions of non-fathers by means of the GLO system are reported, and the application of the GLO system to paternity cases is discussed."}
{"id": "PMID:489027", "title": "beta-Thalassaemia with increased haemoglobin A2 in Turkey. A study of 164 thalassaemic heterozygotes.", "content": "Clinical and haematological findings of 164 Turkish beta-thalassaemic heterozygotes with increased Hb-A2 are presented. The series comprised of 19 children, 81 females and 64 males. The majority of these thalassaemic individuals were asymptomatic and only 4 of them had slight or moderate splenomegaly. The mean values of haemoglobin concentration, PCV, MCH and MCHC were significantly lower than those of the corresponding normal controls. Erythrocytosis was present in 33% of them. Hb-A2 values ranged between 3.8 and 6.1%, and between 3.8 and 6.5% as assessed by the methods of DEAE- or DE-cellulose chromatography, respectively. Hb-F was found to be above the normal range in 15.1%. It is established that beta-thalassaemia with increased Hb-A2 is the most frequent variety of thalassaemia in Turkey (80%) and both beta+- and beta0-thalassaemia genes were present in this country. The regional distribution of beta-thalassaemia in Turkey is also presented and the origin of beta-thalassaemia genes in Turkish people is discussed.", "contents": "beta-Thalassaemia with increased haemoglobin A2 in Turkey. A study of 164 thalassaemic heterozygotes. Clinical and haematological findings of 164 Turkish beta-thalassaemic heterozygotes with increased Hb-A2 are presented. The series comprised of 19 children, 81 females and 64 males. The majority of these thalassaemic individuals were asymptomatic and only 4 of them had slight or moderate splenomegaly. The mean values of haemoglobin concentration, PCV, MCH and MCHC were significantly lower than those of the corresponding normal controls. Erythrocytosis was present in 33% of them. Hb-A2 values ranged between 3.8 and 6.1%, and between 3.8 and 6.5% as assessed by the methods of DEAE- or DE-cellulose chromatography, respectively. Hb-F was found to be above the normal range in 15.1%. It is established that beta-thalassaemia with increased Hb-A2 is the most frequent variety of thalassaemia in Turkey (80%) and both beta+- and beta0-thalassaemia genes were present in this country. The regional distribution of beta-thalassaemia in Turkey is also presented and the origin of beta-thalassaemia genes in Turkish people is discussed."}
{"id": "PMID:489028", "title": "Segregation and linkage analyses of dopamine-beta-hydroxylase activity.", "content": "Dopamine-beta-hydroxylase (DBH) activity in serum was measured by spectrophotometric methods in 95 persons of a large family (HGAR 2), along with 27 polymorphic markers from blood, urine and saliva. The distribution of DBH activity, after appropriate transformation and age adjustment, showed a significantly better fit to a mixture of two normal distributions than a single normal distribution. Pedigree segregation analyses showed evidence of a possible major gene governing low levels of DBH activity, segregating in this family in a recessive fashion. Linkage analyses between that major locus and the 27 polymorphic markers showed no significant lod scores favoring linkage. The highest lod score obtained was 0.81 with Lp at zero recombination fraction. In addition, published data on DBH activity measured by radiochemical assays on 22 families with 161 members were reanalyzed as a quantitative trait, with appropriate correction for ascertainment bias. The results were similar to that of HGAR 2, corroborating the existence of a major locus for DBH activity.", "contents": "Segregation and linkage analyses of dopamine-beta-hydroxylase activity. Dopamine-beta-hydroxylase (DBH) activity in serum was measured by spectrophotometric methods in 95 persons of a large family (HGAR 2), along with 27 polymorphic markers from blood, urine and saliva. The distribution of DBH activity, after appropriate transformation and age adjustment, showed a significantly better fit to a mixture of two normal distributions than a single normal distribution. Pedigree segregation analyses showed evidence of a possible major gene governing low levels of DBH activity, segregating in this family in a recessive fashion. Linkage analyses between that major locus and the 27 polymorphic markers showed no significant lod scores favoring linkage. The highest lod score obtained was 0.81 with Lp at zero recombination fraction. In addition, published data on DBH activity measured by radiochemical assays on 22 families with 161 members were reanalyzed as a quantitative trait, with appropriate correction for ascertainment bias. The results were similar to that of HGAR 2, corroborating the existence of a major locus for DBH activity."}
{"id": "PMID:489029", "title": "Atrichia, abnormal EEG, epilepsy and mental retardation in two sisters.", "content": "Two daughters of a nonconsanguineous couple are described. Both present mental retardation, epileptic seizures, congenital atrichia, histologically anomalous skin and abnormal EEG pattern. From a discussion of the literature on atrichia, the forms without involvement of teeth, nails and hidrosis, among which recessive inheritance prevails, are distinguished from each other. None of them coincide with the syndrome described here.", "contents": "Atrichia, abnormal EEG, epilepsy and mental retardation in two sisters. Two daughters of a nonconsanguineous couple are described. Both present mental retardation, epileptic seizures, congenital atrichia, histologically anomalous skin and abnormal EEG pattern. From a discussion of the literature on atrichia, the forms without involvement of teeth, nails and hidrosis, among which recessive inheritance prevails, are distinguished from each other. None of them coincide with the syndrome described here."}
{"id": "PMID:489030", "title": "A rare electrophoretic enzyme variant of serum alkaline phosphatase in a group of Icelanders.", "content": "An electrophoretic isoenzyme variant of serum alkaline phosphatase was found in 10 out of 343 subjects belonging to an Icelandic population in Husavik and the Husavik region. 9 of the variant-positive subjects were women. The enzyme variant differs from normal isoenzymes in electrophoretic mobility, substrate specificity, and response to inhibitors. It could be demonstrated that nine of the subjects with the enzyme variant were related with each other.", "contents": "A rare electrophoretic enzyme variant of serum alkaline phosphatase in a group of Icelanders. An electrophoretic isoenzyme variant of serum alkaline phosphatase was found in 10 out of 343 subjects belonging to an Icelandic population in Husavik and the Husavik region. 9 of the variant-positive subjects were women. The enzyme variant differs from normal isoenzymes in electrophoretic mobility, substrate specificity, and response to inhibitors. It could be demonstrated that nine of the subjects with the enzyme variant were related with each other."}
{"id": "PMID:489031", "title": "Frequency of glutathione reductase, pyruvate kinase and glucose-6-phosphate dehydrogenase deficiency in a Spanish population.", "content": "The frequencies of deficiencies of glutathione reductase (GSSG-R), pyruvate kinase (PK) and glucose-6-phosphate dehydrogenase (G6PD) in a Spanish sample are presented. A total of 2,129 individuals was analyzed for GSSG-R, 1,636 for PK, and 1,066 (629 males and 437 females) for G6PD. Beutler's method was used. The frequencies obtained for these deficiencies were: GSSG-R, 0.09%; PK, 0.24%; and G6PD, 0.79%. The results are discussed.", "contents": "Frequency of glutathione reductase, pyruvate kinase and glucose-6-phosphate dehydrogenase deficiency in a Spanish population. The frequencies of deficiencies of glutathione reductase (GSSG-R), pyruvate kinase (PK) and glucose-6-phosphate dehydrogenase (G6PD) in a Spanish sample are presented. A total of 2,129 individuals was analyzed for GSSG-R, 1,636 for PK, and 1,066 (629 males and 437 females) for G6PD. Beutler's method was used. The frequencies obtained for these deficiencies were: GSSG-R, 0.09%; PK, 0.24%; and G6PD, 0.79%. The results are discussed."}
{"id": "PMID:489032", "title": "Fingerprint pattern frequencies in schizophrenics. Importance of ethnic origin and plexus visualization score ratings.", "content": "Our previous work with schizophrenics has suggested that the plexus visualization score (PVS), a characteristic derived from in vivo observation of skin capillaries, may be helpful in distinguishing between biologically different groups of patients. Preliminary fingerprint data demonstrated a significant difference in the frequency of whorls between high PVS and low PVS schizophrenics. A survey of fingerprints in control subjects revealed, however, that their ethnic background, even within the presumably 'mixed' Caucasian population of the USA, should also be taken into account. In the present study, the fingerprint pattern frequencies were, therefore, studied in 242 schizophrenics selected on the basis of both their ethnic origin and PVS ratings. Our results demonstrate a statistically significant difference in fingerprint pattern frequencies between high PVS and low PVS schizophrenics, even when they are matched for ethnic origin. The effect of the ethnic factor on fingerprint pattern frequencies in this patient sample is similar to the one previously reported for normal subjects.", "contents": "Fingerprint pattern frequencies in schizophrenics. Importance of ethnic origin and plexus visualization score ratings. Our previous work with schizophrenics has suggested that the plexus visualization score (PVS), a characteristic derived from in vivo observation of skin capillaries, may be helpful in distinguishing between biologically different groups of patients. Preliminary fingerprint data demonstrated a significant difference in the frequency of whorls between high PVS and low PVS schizophrenics. A survey of fingerprints in control subjects revealed, however, that their ethnic background, even within the presumably 'mixed' Caucasian population of the USA, should also be taken into account. In the present study, the fingerprint pattern frequencies were, therefore, studied in 242 schizophrenics selected on the basis of both their ethnic origin and PVS ratings. Our results demonstrate a statistically significant difference in fingerprint pattern frequencies between high PVS and low PVS schizophrenics, even when they are matched for ethnic origin. The effect of the ethnic factor on fingerprint pattern frequencies in this patient sample is similar to the one previously reported for normal subjects."}
{"id": "PMID:489033", "title": "Subtypes of transferrin C.", "content": "Subtypes of transferrin C were studied by means of isoelectric focusing after complete desialylation of transferrin. Family data were consistent with an autosomal co-dominant mode of inheritance. Studies of serum samples from 75 individuals heterozygous for C and another (B or D) variant showed that the genes (C1 and C2) controlling the C subtypes are allelic to the B and D genes. The C2 gene frequency in Swedes and Swedish Lapps was similar to that found previously in Danes and Germans.", "contents": "Subtypes of transferrin C. Subtypes of transferrin C were studied by means of isoelectric focusing after complete desialylation of transferrin. Family data were consistent with an autosomal co-dominant mode of inheritance. Studies of serum samples from 75 individuals heterozygous for C and another (B or D) variant showed that the genes (C1 and C2) controlling the C subtypes are allelic to the B and D genes. The C2 gene frequency in Swedes and Swedish Lapps was similar to that found previously in Danes and Germans."}
{"id": "PMID:489057", "title": "Liposomes in immunology: impairment of the adjuvant effect of liposomes by incorporation of the adjuvant lysolecithin and the role of macrophages.", "content": "The immune response against HSA (human serum albumin) was studied in rabbits after intravenous injection of various HSA preparations. When HSA was injected one day after, together with or coupled to lysolecithin, a late response was found in twelve out of thirteen rabbits, whereas a minority of the rabbits responded when lysolecithin was omitted. These results confirm the adjuvant activity of lysolecithin. A rapid response starting on day 6 was found in rabbits injected with HSA entrapped in liposomes which had been composed of lecithin, phosphatidic acid and cholesterol (PPC liposomes). The response against liposome entrapped HSA was delayed for about one day when the phospholipid adjuvant lysolecithin was incorporated in the liposomes (LPPC liposomes). Results lend support to the hypothesis that the adjuvant activity of lysolecithin and its opposite inhibition of the adjuvant activity of liposomes are mediated by the same mechanism, i.e. inhibition of enzymatic digestion in lysosomes of macrophages.", "contents": "Liposomes in immunology: impairment of the adjuvant effect of liposomes by incorporation of the adjuvant lysolecithin and the role of macrophages. The immune response against HSA (human serum albumin) was studied in rabbits after intravenous injection of various HSA preparations. When HSA was injected one day after, together with or coupled to lysolecithin, a late response was found in twelve out of thirteen rabbits, whereas a minority of the rabbits responded when lysolecithin was omitted. These results confirm the adjuvant activity of lysolecithin. A rapid response starting on day 6 was found in rabbits injected with HSA entrapped in liposomes which had been composed of lecithin, phosphatidic acid and cholesterol (PPC liposomes). The response against liposome entrapped HSA was delayed for about one day when the phospholipid adjuvant lysolecithin was incorporated in the liposomes (LPPC liposomes). Results lend support to the hypothesis that the adjuvant activity of lysolecithin and its opposite inhibition of the adjuvant activity of liposomes are mediated by the same mechanism, i.e. inhibition of enzymatic digestion in lysosomes of macrophages."}
{"id": "PMID:489058", "title": "Nature of human serum blood group T antibodies.", "content": "Antibodies against blood group substance T in normal human sera consist of two types, depending upon whether or not the molecules remain immunochemically active at 37 degrees C. The two agglutinins were isolated by means of T antigen-coupled Sepharose 48 affinity chromatography. Temperature-sensitive agglutinin was eluted from the affinity column at 37 degrees C, while temperature-independent agglutinin remained bound to the antigen. Subsequently, the latter was dissociated from the column in the presence of 2.5 M MgCl2. Examination of six normal sera revealed that the levels of temperature-independent agglutinin was about twice that of temperature-sensitive agglutinin. More than 90% of anti-T agglutinins in normal sera were of the IgM class, in 8 of 11 samples studied. No direct relationship appeared to exist between anti-T titers and blood type. Thermal effects on hemagglutination, at least for anti-T agglutinin interacting with T antigen bearing red cells, is primarily due to the molecular characteristics of the agglutinin and not to the conformational change of the red cell membranes.", "contents": "Nature of human serum blood group T antibodies. Antibodies against blood group substance T in normal human sera consist of two types, depending upon whether or not the molecules remain immunochemically active at 37 degrees C. The two agglutinins were isolated by means of T antigen-coupled Sepharose 48 affinity chromatography. Temperature-sensitive agglutinin was eluted from the affinity column at 37 degrees C, while temperature-independent agglutinin remained bound to the antigen. Subsequently, the latter was dissociated from the column in the presence of 2.5 M MgCl2. Examination of six normal sera revealed that the levels of temperature-independent agglutinin was about twice that of temperature-sensitive agglutinin. More than 90% of anti-T agglutinins in normal sera were of the IgM class, in 8 of 11 samples studied. No direct relationship appeared to exist between anti-T titers and blood type. Thermal effects on hemagglutination, at least for anti-T agglutinin interacting with T antigen bearing red cells, is primarily due to the molecular characteristics of the agglutinin and not to the conformational change of the red cell membranes."}
{"id": "PMID:489059", "title": "The rate of absorption into the embryonic chicken (Gallus domesticus) of 3H-testosterone.", "content": "The maximum absorption of tritiated testosterone by 5-day old embryos occurred one hour after its application to the eggshell. This decreased to relatively low levels during a 77 hour period. When the hormone was applied at the eleventh day of incubation there was a relatively large initial uptake by the bursa, spleen and thymus in that order and a subsequent diminution. Activity absorbed by the spleen remained essentially unchanged throughout the experimental period. The bursa appeared to have the greatest affinity for the hormone.", "contents": "The rate of absorption into the embryonic chicken (Gallus domesticus) of 3H-testosterone. The maximum absorption of tritiated testosterone by 5-day old embryos occurred one hour after its application to the eggshell. This decreased to relatively low levels during a 77 hour period. When the hormone was applied at the eleventh day of incubation there was a relatively large initial uptake by the bursa, spleen and thymus in that order and a subsequent diminution. Activity absorbed by the spleen remained essentially unchanged throughout the experimental period. The bursa appeared to have the greatest affinity for the hormone."}
{"id": "PMID:489060", "title": "The effect of peritoneal macrophages from mice injected with Leishmania braziliensis on the in vitro growth of tumor cells.", "content": "Leishmania braziliensis reportedly is capable of producing a reduction in the growth of solid and ascites murine leukemia and lymphomas. The possibility that the inhibitory effect on the ascites tumor was produced by the activation of the macrophage peritoneal cell population was explored. It was observed that adherent cells from the peritoneal cavity of L. braziliensis injected mice caused a marked inhibitory effect on the in vitro growth of the 6C3HED lymphoma and EL-4 leukemia cells. This effect was dependent on the degree of the macrophage activation, was not produced by supernatants from cultures of activated macrophages, and it seems that contact cell between target and effector cells is necessary. In addition to being cytostatic, this effect was also cytotoxic. The L. braziliensis activated macrophages were also capable of suppressing the multiplication of normal cells induced by mitogen, but this was not observed if the cells were already undergoing multiplication. A similar cytostatic effect on the tumor cells was observed to be produced by the peritoneal non-adherent cell population of the L. braziliensis injected mice.", "contents": "The effect of peritoneal macrophages from mice injected with Leishmania braziliensis on the in vitro growth of tumor cells. Leishmania braziliensis reportedly is capable of producing a reduction in the growth of solid and ascites murine leukemia and lymphomas. The possibility that the inhibitory effect on the ascites tumor was produced by the activation of the macrophage peritoneal cell population was explored. It was observed that adherent cells from the peritoneal cavity of L. braziliensis injected mice caused a marked inhibitory effect on the in vitro growth of the 6C3HED lymphoma and EL-4 leukemia cells. This effect was dependent on the degree of the macrophage activation, was not produced by supernatants from cultures of activated macrophages, and it seems that contact cell between target and effector cells is necessary. In addition to being cytostatic, this effect was also cytotoxic. The L. braziliensis activated macrophages were also capable of suppressing the multiplication of normal cells induced by mitogen, but this was not observed if the cells were already undergoing multiplication. A similar cytostatic effect on the tumor cells was observed to be produced by the peritoneal non-adherent cell population of the L. braziliensis injected mice."}
{"id": "PMID:489090", "title": "Thin-layer chromatographic method for estimation of gamma-aminobutyric acid from brain.", "content": "The present method describes the estimation of gamma-aminobutyric acid (GABA) from adult rat brain by thin layer chromatography (TLC). The major advantage of this TLC method is the sensitivity and repidity of estimation. About 93 to 98 percent recovery is possible by this procedure and this shows linearity up to 9 micrograms. Replicate analysis of GABA by this method shows a co-efficient variation of 1.31%", "contents": "Thin-layer chromatographic method for estimation of gamma-aminobutyric acid from brain. The present method describes the estimation of gamma-aminobutyric acid (GABA) from adult rat brain by thin layer chromatography (TLC). The major advantage of this TLC method is the sensitivity and repidity of estimation. About 93 to 98 percent recovery is possible by this procedure and this shows linearity up to 9 micrograms. Replicate analysis of GABA by this method shows a co-efficient variation of 1.31%"}
{"id": "PMID:489091", "title": "Modulation of vagus escape phenomenon by afferent vagal stimulation.", "content": "The present study was conducted on healthy adult mongrel dogs under chloralose urethane anaesthesia. After bilateral midcervical vagotomy, peripheral end of either vagus was stimulated continuously. This stimulation produced cardiac asystole followed by escape beats. Initially, the heart rate and arterial pressure were low, but both increased progressively and reached a steady-state level in 1-3 min while stimulation of peripheral vagus continued. During this plateau of vagus escape, stimulation of the central end of either vagus produced elevation of arterial pressure and cardio-acceleration. This modulation of vagus escape phenomenon by afferent vagal stimulation was reproducible.", "contents": "Modulation of vagus escape phenomenon by afferent vagal stimulation. The present study was conducted on healthy adult mongrel dogs under chloralose urethane anaesthesia. After bilateral midcervical vagotomy, peripheral end of either vagus was stimulated continuously. This stimulation produced cardiac asystole followed by escape beats. Initially, the heart rate and arterial pressure were low, but both increased progressively and reached a steady-state level in 1-3 min while stimulation of peripheral vagus continued. During this plateau of vagus escape, stimulation of the central end of either vagus produced elevation of arterial pressure and cardio-acceleration. This modulation of vagus escape phenomenon by afferent vagal stimulation was reproducible."}
{"id": "PMID:489092", "title": "Toxicological study of Semecarpus anacardium nut extract.", "content": "Toxicological study was carried out in rats with chloroform-soluble fraction of the nuts of Semecarpus anacardium to determine its safe non-toxic dose. The fraction produced toxicity at all levels tested (50-400 mg/kg) but the extent of toxicity was found dose-dependent. At lower doses this fraction induced partial growth inhibition over 36 days and higher doses proved fatal within 6 days. It was observed that 230 mg/kg caused 50% mortality in rats and this value is 1380 mg/m2 when expressed for body surface area. This work will be of some use in the cancer chemotherapy study of the fraction.", "contents": "Toxicological study of Semecarpus anacardium nut extract. Toxicological study was carried out in rats with chloroform-soluble fraction of the nuts of Semecarpus anacardium to determine its safe non-toxic dose. The fraction produced toxicity at all levels tested (50-400 mg/kg) but the extent of toxicity was found dose-dependent. At lower doses this fraction induced partial growth inhibition over 36 days and higher doses proved fatal within 6 days. It was observed that 230 mg/kg caused 50% mortality in rats and this value is 1380 mg/m2 when expressed for body surface area. This work will be of some use in the cancer chemotherapy study of the fraction."}
{"id": "PMID:489093", "title": "Hormonal influence on epididymal lipids.", "content": "Single dose administration of Prolactin(P), Progesterone (PP) and a combination of both (PPP) affected the epididymal lipids considerably. Caput and Cauda showed differential responses. PP and PPP showed significant alterations in Caput epididymis. However, Prolactin was effective in Cauda epididymis. The importance of these changes in relation to physiological functions of epididymis is discussed.", "contents": "Hormonal influence on epididymal lipids. Single dose administration of Prolactin(P), Progesterone (PP) and a combination of both (PPP) affected the epididymal lipids considerably. Caput and Cauda showed differential responses. PP and PPP showed significant alterations in Caput epididymis. However, Prolactin was effective in Cauda epididymis. The importance of these changes in relation to physiological functions of epididymis is discussed."}
{"id": "PMID:489094", "title": "Effect of ingestion of glucose on fibrinolytic activity and prothrombin time in diabetic and non-diabetic persons.", "content": "Effect of ingestion of 50 g of glucose on euglobulin clot lysis time and prothrombin time was studied in 14 diabetic and 12 non-diabetic persons. There was no significant change in euglobulin clot lysis time and prothrombin time after ingestion of glucose in the non-diabetic group. In the diabetic group, ingestion of glucose resulted in significant increase in euglobulin clot lysis time at the end of one hr which returned to initial level at the end of two hours even though blood glucose level at two hrs was still higher than fasting level. Increase in clot lysis time has no correlation to the blood glucose level. There was no significant change in prothrombin time after glucose ingestion.", "contents": "Effect of ingestion of glucose on fibrinolytic activity and prothrombin time in diabetic and non-diabetic persons. Effect of ingestion of 50 g of glucose on euglobulin clot lysis time and prothrombin time was studied in 14 diabetic and 12 non-diabetic persons. There was no significant change in euglobulin clot lysis time and prothrombin time after ingestion of glucose in the non-diabetic group. In the diabetic group, ingestion of glucose resulted in significant increase in euglobulin clot lysis time at the end of one hr which returned to initial level at the end of two hours even though blood glucose level at two hrs was still higher than fasting level. Increase in clot lysis time has no correlation to the blood glucose level. There was no significant change in prothrombin time after glucose ingestion."}
{"id": "PMID:489095", "title": "Effects of neuromuscular blocking agents on arterial blood pressure in the rat.", "content": "Gallamine triethiodide pancuronium bromide or d-tubocurarine was infused intravenously at different rates in urethane anaesthetized rats. When given in neuromuscular blocking doses gallamine triethiodide produced significant hypotension if the rate of infusion exceeded 2 mg/min whereas neither pancuronium bromide nor d-tubocurarine produced a marked change in blood pressure in neuromuscular blocking doses.", "contents": "Effects of neuromuscular blocking agents on arterial blood pressure in the rat. Gallamine triethiodide pancuronium bromide or d-tubocurarine was infused intravenously at different rates in urethane anaesthetized rats. When given in neuromuscular blocking doses gallamine triethiodide produced significant hypotension if the rate of infusion exceeded 2 mg/min whereas neither pancuronium bromide nor d-tubocurarine produced a marked change in blood pressure in neuromuscular blocking doses."}
{"id": "PMID:489096", "title": "A simple gravimetric method for estimation of plasma fibrinogen.", "content": "The study was undertaken to evaluate a new method of estimating plasma fibrinogen levels, gravimetrically using calcium chloride in albino rats. The results were compared with the standard method in which thrombin was used as clotting agent and were found to be statistically not significant. Advantages of using calcium chloride instead of thrombin have been discussed.", "contents": "A simple gravimetric method for estimation of plasma fibrinogen. The study was undertaken to evaluate a new method of estimating plasma fibrinogen levels, gravimetrically using calcium chloride in albino rats. The results were compared with the standard method in which thrombin was used as clotting agent and were found to be statistically not significant. Advantages of using calcium chloride instead of thrombin have been discussed."}
{"id": "PMID:489100", "title": "Effect of tonicity on glucose absorption and histomorphology of rat intestinal mucosa.", "content": "Perfusion of the gut lumen with 2 ml of isotonic solutions showed uniform absorption of glucose and water over the four 15-minutes study periods, the absorption being maximum in jejunum and least in colon. With hypertonic solutions the glucose absorption decrease was related to the increasing osmolarity and duration of exposure to the solution as well as the nature of the substance used. Histological changes reflecting impaired functions were localised to the tips of the villi and were observed on exposure to hypertonic solution for 45 minutes or more.", "contents": "Effect of tonicity on glucose absorption and histomorphology of rat intestinal mucosa. Perfusion of the gut lumen with 2 ml of isotonic solutions showed uniform absorption of glucose and water over the four 15-minutes study periods, the absorption being maximum in jejunum and least in colon. With hypertonic solutions the glucose absorption decrease was related to the increasing osmolarity and duration of exposure to the solution as well as the nature of the substance used. Histological changes reflecting impaired functions were localised to the tips of the villi and were observed on exposure to hypertonic solution for 45 minutes or more."}
{"id": "PMID:489122", "title": "Essential dependence of smooth surface caries on, and augmentation of fissure caries by, sucrose and Streptococcus mutans infection.", "content": "Streptococcus mutans-free Osborne-Mendel rats were used to study the ability of well-characterized S. mutans strains of Bratthall serotypes c, d, and E to form plaque and cause caries when the animals consumed either sucrose- or glucose-containing diets. All of the serotype representatives successfully infected, colonized, and emerged in the oral ecology of animals, independent of the carbohydrate supplementation of the diet. However, the sucrose-containing diet supported higher percentages of S. mutans of all the serotypes in the plaque and greater amounts of plaque on the teeth. Smooth surface caries was essentially S. mutans dependent and sucrose dependent; fissure caries, although it was neither dependent on S. mutans infection nor sucrose consumption, was augmented by both. This sucrose-associated emergence of all three serotype representatives in the plaque flora and their virulence in the production of caries can be ascribed to their production of alkali-solible alpha-(1 yields 3)-rich glucans from sucrose.", "contents": "Essential dependence of smooth surface caries on, and augmentation of fissure caries by, sucrose and Streptococcus mutans infection. Streptococcus mutans-free Osborne-Mendel rats were used to study the ability of well-characterized S. mutans strains of Bratthall serotypes c, d, and E to form plaque and cause caries when the animals consumed either sucrose- or glucose-containing diets. All of the serotype representatives successfully infected, colonized, and emerged in the oral ecology of animals, independent of the carbohydrate supplementation of the diet. However, the sucrose-containing diet supported higher percentages of S. mutans of all the serotypes in the plaque and greater amounts of plaque on the teeth. Smooth surface caries was essentially S. mutans dependent and sucrose dependent; fissure caries, although it was neither dependent on S. mutans infection nor sucrose consumption, was augmented by both. This sucrose-associated emergence of all three serotype representatives in the plaque flora and their virulence in the production of caries can be ascribed to their production of alkali-solible alpha-(1 yields 3)-rich glucans from sucrose."}
{"id": "PMID:489123", "title": "Purification and biochemical characterization of deoxythymidine kinase of deoxythymidine kinase-deficient mouse 3T3 cells biochemically transformed by equine herpesvirus type 1.", "content": "A line of mouse 3T3 cells lacking deoxythymidine kinase (dTK-) was stably transformed to see dTK+ phenotype after exposure to ultraviolet-irradiated equine herpesvirus type 1 (EHV-1). Deoxythymidine kinase (dTK) was purified from the biochemically transformed mouse cells by affinity chromatography on deoxythymidine-Sepharose. The purified dTK from EHV-1-transformed 3T3 cells was identical to the dTK purified from dTK- 3T3 cells lytically infected with EHV-1 with respect to its electrophoretic mobility, molecular weight, substrate specificity, phosphate donor specificity, and immunological specificity. The sedimentation velocity of the purified dTK from the transformed 3T3 cells was similar to that previously reported for the enzyme in lytically infected dTK- 3T3 cells, and its molecular weight was estimated to be 87,000. Antiserum prepared against the EHV-1 dTK induced in horse cells inactivated the dTK purified from the transformed mouse cells. The Km for deoxythymidine (5 micrometers) of purified dTK from the EHV-1-transformed cells was the same as that reported for the EHV-1-induced dTK. These results further support the notion that the dTK acquired by dTK- mouse 3T3 cells after transformation by EHV-1 is of viral and not of cellular origin.", "contents": "Purification and biochemical characterization of deoxythymidine kinase of deoxythymidine kinase-deficient mouse 3T3 cells biochemically transformed by equine herpesvirus type 1. A line of mouse 3T3 cells lacking deoxythymidine kinase (dTK-) was stably transformed to see dTK+ phenotype after exposure to ultraviolet-irradiated equine herpesvirus type 1 (EHV-1). Deoxythymidine kinase (dTK) was purified from the biochemically transformed mouse cells by affinity chromatography on deoxythymidine-Sepharose. The purified dTK from EHV-1-transformed 3T3 cells was identical to the dTK purified from dTK- 3T3 cells lytically infected with EHV-1 with respect to its electrophoretic mobility, molecular weight, substrate specificity, phosphate donor specificity, and immunological specificity. The sedimentation velocity of the purified dTK from the transformed 3T3 cells was similar to that previously reported for the enzyme in lytically infected dTK- 3T3 cells, and its molecular weight was estimated to be 87,000. Antiserum prepared against the EHV-1 dTK induced in horse cells inactivated the dTK purified from the transformed mouse cells. The Km for deoxythymidine (5 micrometers) of purified dTK from the EHV-1-transformed cells was the same as that reported for the EHV-1-induced dTK. These results further support the notion that the dTK acquired by dTK- mouse 3T3 cells after transformation by EHV-1 is of viral and not of cellular origin."}
{"id": "PMID:489124", "title": "Interferon production: variation in yields from human cell lines.", "content": "A survey of human diploid, aneusomic, transformed and tumor fibroblast or fibroblastoid cell lines for their capacity to produce interferon after polyriboinosinic acid:polycytidylic acid induction showed considerable variation in responsiveness. There was no apparent correlation between karyotype or phenotype and interferon production. Pretreating or \"priming\" the cells with human interferon generally led to increased yields of interferon after polyriboinosinic acid:polycytidylic acid induction in all cell lines tested. All the cells also showed the \"super-induction\" phenomenon, although to varying degrees. The combination of priming and superinduction conditions led to the production of very high yields of interferon in some cell lines, but in other lines, yields less than either the primed or superinduced amounts were found. A more limited survey of human cell lines for their capacity to produce interferon after Newcastle disease virus induction also showed that yields varied from line to line. However, there was little evidence to suggest that ability to produce interferon after Newcastle disease virus induction correlated with that after polyriboinosinic acid:polycytidylic acid induction.", "contents": "Interferon production: variation in yields from human cell lines. A survey of human diploid, aneusomic, transformed and tumor fibroblast or fibroblastoid cell lines for their capacity to produce interferon after polyriboinosinic acid:polycytidylic acid induction showed considerable variation in responsiveness. There was no apparent correlation between karyotype or phenotype and interferon production. Pretreating or \"priming\" the cells with human interferon generally led to increased yields of interferon after polyriboinosinic acid:polycytidylic acid induction in all cell lines tested. All the cells also showed the \"super-induction\" phenomenon, although to varying degrees. The combination of priming and superinduction conditions led to the production of very high yields of interferon in some cell lines, but in other lines, yields less than either the primed or superinduced amounts were found. A more limited survey of human cell lines for their capacity to produce interferon after Newcastle disease virus induction also showed that yields varied from line to line. However, there was little evidence to suggest that ability to produce interferon after Newcastle disease virus induction correlated with that after polyriboinosinic acid:polycytidylic acid induction."}
{"id": "PMID:489125", "title": "Listeria pneumonitis: influence of route of immunization on resistance to airborne infection.", "content": "Mice that are immunized with an airborne inoculum of BCG are more highly resistant to airborne challenge with Mycobacterium tuberculosis than are mice that are immunized by the subcutaneous or intravenous route. To discover whether this phenomenon is peculiar to tuberculosis, we studied the influence of the route of immunization upon pulmonary resistance in Listeria monocytogenes infection. Mice were immunized by the airborne, intravenous, or footpad route and were subsequently challenged by the same route at 1 to 4 weeks after immunization. Mice were highly and uniformly resistant to intravenous challenge, regardless of the route of immunization. The route of immunization bore no influence upon resistance to footpad infection, but resistance was appreciably better in mice challenged within 2 weeks of immunization than it was at later time points. In mice immunized by the footpad and intravenous routes, the pattern of resistance to airborne and footpad challenges was similar, in that there was substantially less immunity at 4 weeks than at 2 weeks after immunization. However, mice immunized by the airborne route were highly resistant to airborne challenge, regardless of the interval between immunization and reinfection. In this last respect, resistance of the lungs to reinfection was similar after Listeria and tuberculosis pneumonitis. It is suggested that a similar pattern of resistance may prevail in pneumonitis caused by other facultative intracellular parasites.", "contents": "Listeria pneumonitis: influence of route of immunization on resistance to airborne infection. Mice that are immunized with an airborne inoculum of BCG are more highly resistant to airborne challenge with Mycobacterium tuberculosis than are mice that are immunized by the subcutaneous or intravenous route. To discover whether this phenomenon is peculiar to tuberculosis, we studied the influence of the route of immunization upon pulmonary resistance in Listeria monocytogenes infection. Mice were immunized by the airborne, intravenous, or footpad route and were subsequently challenged by the same route at 1 to 4 weeks after immunization. Mice were highly and uniformly resistant to intravenous challenge, regardless of the route of immunization. The route of immunization bore no influence upon resistance to footpad infection, but resistance was appreciably better in mice challenged within 2 weeks of immunization than it was at later time points. In mice immunized by the footpad and intravenous routes, the pattern of resistance to airborne and footpad challenges was similar, in that there was substantially less immunity at 4 weeks than at 2 weeks after immunization. However, mice immunized by the airborne route were highly resistant to airborne challenge, regardless of the interval between immunization and reinfection. In this last respect, resistance of the lungs to reinfection was similar after Listeria and tuberculosis pneumonitis. It is suggested that a similar pattern of resistance may prevail in pneumonitis caused by other facultative intracellular parasites."}
{"id": "PMID:489126", "title": "Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections.", "content": "Combinations of bacteria isolated from the root canals of teeth with necrotic pulps and periapical bone destruction were tested for their capacity to induce abscess formation and transmissible infections when inoculated subcutaneously into guinea pigs. Transmissible infections could be induced with combinations obtained from teeth with purulent apical inflammation, but not with combinations from symptomless teeth with chronic apical inflammation. All combinations which gave transmissible infections contained strains of Bacteroides melaninogenicus or B. asaccharolyticus (formerly B. melaninogenicus subsp. asaccharolyticus). The results suggest that purulent inflammation in the apical region in certain cases may be induced by specific combinations of bacteria in the root canal and that the presence of B. melaninogenicus or B. asaccharolyticus in such combinations is essential. However, with one exception, the strains needed the support of additional microorganisms to achieve pathogenicity. The results indicate that Peptostreptococcus micros was also essential. Histological sections of the lesions in the guinea pigs showed that all bacterial combinations induced acute inflammation with an accumulation of polymorphonuclear leukocytes and the formation of an abscess. However, the presence of B. melaninogenicus or B.  asaccharolyticus in the combinations resulted in a failure of abscess resolution, with a gradually increaseing accumulation of polymorphonuclear leukocytes.", "contents": "Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections. Combinations of bacteria isolated from the root canals of teeth with necrotic pulps and periapical bone destruction were tested for their capacity to induce abscess formation and transmissible infections when inoculated subcutaneously into guinea pigs. Transmissible infections could be induced with combinations obtained from teeth with purulent apical inflammation, but not with combinations from symptomless teeth with chronic apical inflammation. All combinations which gave transmissible infections contained strains of Bacteroides melaninogenicus or B. asaccharolyticus (formerly B. melaninogenicus subsp. asaccharolyticus). The results suggest that purulent inflammation in the apical region in certain cases may be induced by specific combinations of bacteria in the root canal and that the presence of B. melaninogenicus or B. asaccharolyticus in such combinations is essential. However, with one exception, the strains needed the support of additional microorganisms to achieve pathogenicity. The results indicate that Peptostreptococcus micros was also essential. Histological sections of the lesions in the guinea pigs showed that all bacterial combinations induced acute inflammation with an accumulation of polymorphonuclear leukocytes and the formation of an abscess. However, the presence of B. melaninogenicus or B.  asaccharolyticus in the combinations resulted in a failure of abscess resolution, with a gradually increaseing accumulation of polymorphonuclear leukocytes."}
{"id": "PMID:489127", "title": "Mechanisms of Listeria monocytogenes-mediated modulation of tumor growth.", "content": "Studies using Listeria monocytogenes as an antitumor agent were initiated to determine the requirements for Listeria-mediated tumor inhibition to occur. When Strain 13 guinea pigs were injected with an admixture of viable Listeria and a methylcholanthrene-induced fibrosarcoma in a ratio of 1 bacterium to 100 tumor cells, Listeria had a marked capacity to inhibit tumor growth. This confirms an earlier study in our laboratory (M. M. Dustoor, A. Fulton, W. Croft, and A. A. Blazkovec, Infect. Immun. 23:54-60, 1979). At no time did animals exhibit overt symptoms of disease as a result of Listeria infection. Animals treated with antilymphocyte serum, which had previously been shown to abrogate T-cell functions, were no longer able to suppress Listeria-tumor cell mixtures. Treatment in vivo with carrageenan, a macrophage-inhibitory agent, also abrogated Listeria-mediated tumor inhibition. These results suggest that Listeria-mediated inhibition requires intact T-lymphocyte and macrophage function. Experiments in which Listeria was given in admixture with the tumor cells or in the opposite flank demonstrated that the antitumor effects require intimate association of the Listeria and tumor cells. Histopathological studies, showing that macrophages and lymphocytes are the predominant inflammatory cells present at sites of tumor destruction, further suggest a role for these cells in Listeria-mediated inhibition. Animals which had rejected prior Listeria-tumor cell inocula were resistant to rechallenge with the homologous tumor for more than 1 year. This work thus confirms in vitro studies demonstrating that both lymphocytes and macrophages are required for Listeria-mediated tumor inhibition to occur. This study demonstrates that viable Listeria can have potent antitumor effects without causing overt disease as a result of Listeria infection.", "contents": "Mechanisms of Listeria monocytogenes-mediated modulation of tumor growth. Studies using Listeria monocytogenes as an antitumor agent were initiated to determine the requirements for Listeria-mediated tumor inhibition to occur. When Strain 13 guinea pigs were injected with an admixture of viable Listeria and a methylcholanthrene-induced fibrosarcoma in a ratio of 1 bacterium to 100 tumor cells, Listeria had a marked capacity to inhibit tumor growth. This confirms an earlier study in our laboratory (M. M. Dustoor, A. Fulton, W. Croft, and A. A. Blazkovec, Infect. Immun. 23:54-60, 1979). At no time did animals exhibit overt symptoms of disease as a result of Listeria infection. Animals treated with antilymphocyte serum, which had previously been shown to abrogate T-cell functions, were no longer able to suppress Listeria-tumor cell mixtures. Treatment in vivo with carrageenan, a macrophage-inhibitory agent, also abrogated Listeria-mediated tumor inhibition. These results suggest that Listeria-mediated inhibition requires intact T-lymphocyte and macrophage function. Experiments in which Listeria was given in admixture with the tumor cells or in the opposite flank demonstrated that the antitumor effects require intimate association of the Listeria and tumor cells. Histopathological studies, showing that macrophages and lymphocytes are the predominant inflammatory cells present at sites of tumor destruction, further suggest a role for these cells in Listeria-mediated inhibition. Animals which had rejected prior Listeria-tumor cell inocula were resistant to rechallenge with the homologous tumor for more than 1 year. This work thus confirms in vitro studies demonstrating that both lymphocytes and macrophages are required for Listeria-mediated tumor inhibition to occur. This study demonstrates that viable Listeria can have potent antitumor effects without causing overt disease as a result of Listeria infection."}
{"id": "PMID:489129", "title": "Interaction of influenza A virus with human peripheral blood lymphocytes.", "content": "Peripheral blood lymphocytes cultured in the presence of phytohemagglutinin, concanavalin A, or pokeweed mitogen were exposed in vitro to influenza A virus. The synthesis of several virus-specific proteins, including the nucleoprotein, membrane protein, and nonstructural 1 protein were detected, although no infectious virus was produced by the lymphocyte cultures. Evidence was obtained that only a subpopulation of mitogen-transformed cells would support virus protein synthesis. A comparison of the interactions of influenza A virus with lymphocytes from normal individuals and from rheumatoid arthritis patients showed that the same range of virus-specific proteins were made, in similar quantities, regardless of the source of lymphocytes.", "contents": "Interaction of influenza A virus with human peripheral blood lymphocytes. Peripheral blood lymphocytes cultured in the presence of phytohemagglutinin, concanavalin A, or pokeweed mitogen were exposed in vitro to influenza A virus. The synthesis of several virus-specific proteins, including the nucleoprotein, membrane protein, and nonstructural 1 protein were detected, although no infectious virus was produced by the lymphocyte cultures. Evidence was obtained that only a subpopulation of mitogen-transformed cells would support virus protein synthesis. A comparison of the interactions of influenza A virus with lymphocytes from normal individuals and from rheumatoid arthritis patients showed that the same range of virus-specific proteins were made, in similar quantities, regardless of the source of lymphocytes."}
{"id": "PMID:489128", "title": "In vitro studies of dental plaque formation: adsorption of oral streptococci to hydroxyaptite.", "content": "A mixture of saliva-coated hydroxyapatite beads and radioactively labeled bacteria has been employed as an in vitro model for the initial phase of dental plaque formation. Adsorption in this model can be expressed by the Langmuir adsorption isotherm, and the adherence of oral streptococci can be expressed as the product of the affinity constant (Ka) and the number of binding sites (N), KaN. With this approach, Streptococcus sanguis serotype 1 strains adhered better (KaN = [187 +/- 72] X 10(-2)) than serotype 2 strains (KaN = [97 +/- 84] X 10(-2)); a t test showed this difference to be statistically significant to the 99.99% confidence level. Strains of S. mitis, S. mutans, and S. salivarius did not appear to adhere as well. To analyze the bacterial receptors involved in adherence, competition studies in which increasing quantities of unlabeled bacteria were added to a fixed quantity (4 X 10(9) cells per ml) of 3H-labeled serotype 1, reference strain S. sanguis G9B, were performed. These studies indicated that the type 1 strains competed for the same, or closely related, binding sites. Competition studies using serotype 2 S. sanguis strains resulted in an increased binding of reference strain G9B to hydroxyapatite. Scanning electron microscopy indicated this effect was due to the formation of localized aggregations of bacteria, presumably representing the two bacterial types. The results of competition studies with S. mitis were variable, and several strains of other oral bacteria showed little or no competition.", "contents": "In vitro studies of dental plaque formation: adsorption of oral streptococci to hydroxyaptite. A mixture of saliva-coated hydroxyapatite beads and radioactively labeled bacteria has been employed as an in vitro model for the initial phase of dental plaque formation. Adsorption in this model can be expressed by the Langmuir adsorption isotherm, and the adherence of oral streptococci can be expressed as the product of the affinity constant (Ka) and the number of binding sites (N), KaN. With this approach, Streptococcus sanguis serotype 1 strains adhered better (KaN = [187 +/- 72] X 10(-2)) than serotype 2 strains (KaN = [97 +/- 84] X 10(-2)); a t test showed this difference to be statistically significant to the 99.99% confidence level. Strains of S. mitis, S. mutans, and S. salivarius did not appear to adhere as well. To analyze the bacterial receptors involved in adherence, competition studies in which increasing quantities of unlabeled bacteria were added to a fixed quantity (4 X 10(9) cells per ml) of 3H-labeled serotype 1, reference strain S. sanguis G9B, were performed. These studies indicated that the type 1 strains competed for the same, or closely related, binding sites. Competition studies using serotype 2 S. sanguis strains resulted in an increased binding of reference strain G9B to hydroxyapatite. Scanning electron microscopy indicated this effect was due to the formation of localized aggregations of bacteria, presumably representing the two bacterial types. The results of competition studies with S. mitis were variable, and several strains of other oral bacteria showed little or no competition."}
{"id": "PMID:489130", "title": "Experimental infection in mice with Treponema hyodysenteriae.", "content": "Nineteen of 22 female mice (CF1 strain) inoculated intragastrically with Treponema hyodysenteriae developed cecal and colonic lesions consisting of catarrhal inflammation, edema, and occasional hemorrhage.", "contents": "Experimental infection in mice with Treponema hyodysenteriae. Nineteen of 22 female mice (CF1 strain) inoculated intragastrically with Treponema hyodysenteriae developed cecal and colonic lesions consisting of catarrhal inflammation, edema, and occasional hemorrhage."}
{"id": "PMID:489131", "title": "Adsorption and growth of Vibrio cholerae on chitin.", "content": "Incubation of Vibrio cholerae of O-group serotype 1 with chitin particles resulted in adsorption of vibrios onto chitin; chitin-adsorbed V. cholerae survived exposure to acid better than nonadsorbed vibrios. V. cholerae multiplied in dialyzed chitin suspended in 4.2% NaCl, suggesting that adherence to ingested chitin of crustacea might be of epidemiological significance by providing a substrate for vibrio multiplication as well as protection from gastric acid during stomach transit.", "contents": "Adsorption and growth of Vibrio cholerae on chitin. Incubation of Vibrio cholerae of O-group serotype 1 with chitin particles resulted in adsorption of vibrios onto chitin; chitin-adsorbed V. cholerae survived exposure to acid better than nonadsorbed vibrios. V. cholerae multiplied in dialyzed chitin suspended in 4.2% NaCl, suggesting that adherence to ingested chitin of crustacea might be of epidemiological significance by providing a substrate for vibrio multiplication as well as protection from gastric acid during stomach transit."}
{"id": "PMID:489132", "title": "Liposomal enhancement of the immunogenicity of adenovirus type 5 hexon and fiber vaccines.", "content": "Immunogenicity of adenovirus capsid proteins carried in liposomes was comparable to that with equivalent doses administered in Freund adjuvant, and both forms were more potent than aqueous vaccines.", "contents": "Liposomal enhancement of the immunogenicity of adenovirus type 5 hexon and fiber vaccines. Immunogenicity of adenovirus capsid proteins carried in liposomes was comparable to that with equivalent doses administered in Freund adjuvant, and both forms were more potent than aqueous vaccines."}
{"id": "PMID:489133", "title": "Depression of contact hypersensitivity to oxazolone in mice exposed to Newcastle disease virus.", "content": "The effect of Newcastle disease virus (NDV) on delayed hypersensitivity to oxazolone in CBA mice was studied. There was a significant impairment of the ability of mice to develop cutaneous hypersensitivity shortly after injection of the virus. The effect was evident when NDV was administered up to 2 days before or within 24 h after sensitization, suggesting that NDV interferes with the process of sensitization. The degree of depression was related to the dose of virus inoculated. NDV inactivated by UV irradiation or heat did not depress contact sensitivity to oxazolone. These data are considered to support the hypothesis that the depression is mediated by a direct interaction between lymphocytes and NDV.", "contents": "Depression of contact hypersensitivity to oxazolone in mice exposed to Newcastle disease virus. The effect of Newcastle disease virus (NDV) on delayed hypersensitivity to oxazolone in CBA mice was studied. There was a significant impairment of the ability of mice to develop cutaneous hypersensitivity shortly after injection of the virus. The effect was evident when NDV was administered up to 2 days before or within 24 h after sensitization, suggesting that NDV interferes with the process of sensitization. The degree of depression was related to the dose of virus inoculated. NDV inactivated by UV irradiation or heat did not depress contact sensitivity to oxazolone. These data are considered to support the hypothesis that the depression is mediated by a direct interaction between lymphocytes and NDV."}
{"id": "PMID:489134", "title": "Quantitation of immediate and delayed hypersensitivity responses in Trichinella-infected mice. Correlation with worm expulsion.", "content": "The developmental relationships between active cutaneous or local anaphylaxis, delayed hypersensitivity and worm expulsion were quantitatively examined in Trichinella-infected mice. The onset of both types of hypersensitivities and increase in sensitivity to antigen following an initial infection correlated with the onset and rate of elimination of adult worms. Mice passively sensitized with serum containing both IgG1 and IgE antibodies, but not IgG1 alone, expelled their worms at a faster rate in comparison to the controls. On the basis of these findings it is suggested that local allergic reactions mediated by anaphylactic antibodies are involved in the development of resistance to Trichinella infection.", "contents": "Quantitation of immediate and delayed hypersensitivity responses in Trichinella-infected mice. Correlation with worm expulsion. The developmental relationships between active cutaneous or local anaphylaxis, delayed hypersensitivity and worm expulsion were quantitatively examined in Trichinella-infected mice. The onset of both types of hypersensitivities and increase in sensitivity to antigen following an initial infection correlated with the onset and rate of elimination of adult worms. Mice passively sensitized with serum containing both IgG1 and IgE antibodies, but not IgG1 alone, expelled their worms at a faster rate in comparison to the controls. On the basis of these findings it is suggested that local allergic reactions mediated by anaphylactic antibodies are involved in the development of resistance to Trichinella infection."}
{"id": "PMID:489135", "title": "Enhancement of eosinophil leukocyte chemotaxis by a factor in normal human serum (SEF).", "content": "An activity in normal human serum is described which enhances the migration of eosinophils towards neutrophil-derived eosinophil chemotactic factor (ECF). The serum-enhancing factor (SEF), in contrast to the inhibitors in serum, is expressed primarily during weak chemotaxis, acts in a cell-directed fashion, increases the chemokinesis of eosinophils and, in the presence of ECF, enhances chemotaxis in a synergistic fashion. SEF is found in sera of several mammalian species, affects human and guinea pig eosinophils, and it is heat-labile (56 degrees C). On Sephadex column chromatography, SEF has a molecular weight of approximately 800,000 daltons. In some sera, a second activity with a molecular weight of approximately 200,000 daltons is eluted. SEF does not enhance eosinophil migration once the cells have been deactivated in vitro by their chemotactic factor. Because of its wide distribution, SEF may play an important modulating role both in in vitro assay systems and in vivo.", "contents": "Enhancement of eosinophil leukocyte chemotaxis by a factor in normal human serum (SEF). An activity in normal human serum is described which enhances the migration of eosinophils towards neutrophil-derived eosinophil chemotactic factor (ECF). The serum-enhancing factor (SEF), in contrast to the inhibitors in serum, is expressed primarily during weak chemotaxis, acts in a cell-directed fashion, increases the chemokinesis of eosinophils and, in the presence of ECF, enhances chemotaxis in a synergistic fashion. SEF is found in sera of several mammalian species, affects human and guinea pig eosinophils, and it is heat-labile (56 degrees C). On Sephadex column chromatography, SEF has a molecular weight of approximately 800,000 daltons. In some sera, a second activity with a molecular weight of approximately 200,000 daltons is eluted. SEF does not enhance eosinophil migration once the cells have been deactivated in vitro by their chemotactic factor. Because of its wide distribution, SEF may play an important modulating role both in in vitro assay systems and in vivo."}
{"id": "PMID:489136", "title": "Search for specific effector functions of C3H X CBA lymphocytes which have proliferated in the spleens of irradiated CBA mice.", "content": "Injection of C3H X CBA hybrid lymphocytes into CBA mice specifically reduces the pool of host T cells which are reactive against the Mls antigen determined by the C3H genome. Since C3H X CBA lymphocytes are triggered to cell division in the spleens of irradiated CBA mice we have now examined if such activated cell populations exhibit any effector functions against CBA lymphocytes which are reactive against C3H-determined antigens. It was observed that such 'educated' cell populations were unable to significantly kill CBA lymphocytes which are triggered to cell division in C3H X CBA hosts or CBA lymphocytes which can inhibit proliferation of transplanted C3H X CBA bone marrow cells. Moreover, there was no evidence that such 'educated' cell populations can specifically kill anti-C3H-reactive CBA lymphocytes in vitro or damage CBA bone marrow cells in vivo. Thus, these results do not demonstrate that C3H X CBA lymphocytes acquire any effector functions against CBA T cells which are reactive against C3H-determined antigens.", "contents": "Search for specific effector functions of C3H X CBA lymphocytes which have proliferated in the spleens of irradiated CBA mice. Injection of C3H X CBA hybrid lymphocytes into CBA mice specifically reduces the pool of host T cells which are reactive against the Mls antigen determined by the C3H genome. Since C3H X CBA lymphocytes are triggered to cell division in the spleens of irradiated CBA mice we have now examined if such activated cell populations exhibit any effector functions against CBA lymphocytes which are reactive against C3H-determined antigens. It was observed that such 'educated' cell populations were unable to significantly kill CBA lymphocytes which are triggered to cell division in C3H X CBA hosts or CBA lymphocytes which can inhibit proliferation of transplanted C3H X CBA bone marrow cells. Moreover, there was no evidence that such 'educated' cell populations can specifically kill anti-C3H-reactive CBA lymphocytes in vitro or damage CBA bone marrow cells in vivo. Thus, these results do not demonstrate that C3H X CBA lymphocytes acquire any effector functions against CBA T cells which are reactive against C3H-determined antigens."}
{"id": "PMID:489137", "title": "A microcytotoxicity assay using 51Cr for measuring specific cell-mediated and humoral immune responses.", "content": "A microcytotoxicity assay (MCA) for measuring in vitro cell-mediated cytotoxicity (CMC) and humoral cytotoxicity which uses 51Cr-labelled target cells is described. Data is presented to show the uptake and rentention of 51Cr by various viable cultured cells. The CMC determined by 51Cr-labelled target cell retention in microtest II plate wells was verified by microscopic observation. Comparisons between the CMC observed 24 and 48 h after incubation of immune and normal lymphoid cells with 51Cr-labelled target cells were made. The factors, including savings in time and expense, which may make this modified MCA superior to other MCAs in use, are discussed.", "contents": "A microcytotoxicity assay using 51Cr for measuring specific cell-mediated and humoral immune responses. A microcytotoxicity assay (MCA) for measuring in vitro cell-mediated cytotoxicity (CMC) and humoral cytotoxicity which uses 51Cr-labelled target cells is described. Data is presented to show the uptake and rentention of 51Cr by various viable cultured cells. The CMC determined by 51Cr-labelled target cell retention in microtest II plate wells was verified by microscopic observation. Comparisons between the CMC observed 24 and 48 h after incubation of immune and normal lymphoid cells with 51Cr-labelled target cells were made. The factors, including savings in time and expense, which may make this modified MCA superior to other MCAs in use, are discussed."}
{"id": "PMID:489138", "title": "Methdological issues in life-span developmental psychology from a dialectical perspective.", "content": "In the last few years there have been several studies reported in the literature using bifactorial ANOVA designs for the description and/or explanation of developmental phenomena. As formulated by Schaie and Baltes, these bifactorial ANOVA designs consist of taking any two of age, cohort, or time of measurement as the independent variables. The logic of these designs is carefully considered with respect to answering descriptive and/or explanatory developmental questions. It is concluded that the employment of ANOVA in the manner recommended by Schaie and Baltes has led us down a blind alley with respect to addressing developmental phenomena. Much more reasonable data analysis designs are available for getting at the description and/or explanation of development, namely, regression techniques. The advantages of regression models as compared to ANOVA are discussed in the context of developmental data analysis. Disenagement theory is singled out for special attention in the context of the dialectic between the individual and the environment.", "contents": "Methdological issues in life-span developmental psychology from a dialectical perspective. In the last few years there have been several studies reported in the literature using bifactorial ANOVA designs for the description and/or explanation of developmental phenomena. As formulated by Schaie and Baltes, these bifactorial ANOVA designs consist of taking any two of age, cohort, or time of measurement as the independent variables. The logic of these designs is carefully considered with respect to answering descriptive and/or explanatory developmental questions. It is concluded that the employment of ANOVA in the manner recommended by Schaie and Baltes has led us down a blind alley with respect to addressing developmental phenomena. Much more reasonable data analysis designs are available for getting at the description and/or explanation of development, namely, regression techniques. The advantages of regression models as compared to ANOVA are discussed in the context of developmental data analysis. Disenagement theory is singled out for special attention in the context of the dialectic between the individual and the environment."}
{"id": "PMID:489139", "title": "Foster-family care for the elderly: surrogate family or mini-institution?", "content": "The rationale behind adult foster care (AFC) has been that \"participation in the life of the family\" is superior to institutionalization. The extent to which AFC is familial has been widely debated, ranging from claims that this environment provides a surrogate family, to claims that it is no more than a mini-institution. This paper discusses the extent to which elderly clients are integrated into the family and the method by which such integration was measured in a sample of one hundred adult foster homes in New York State. The four dimensions used to measure familism were Affection, Social Interaction, the performance of Ritual, and the minimization of Social Distance.", "contents": "Foster-family care for the elderly: surrogate family or mini-institution? The rationale behind adult foster care (AFC) has been that \"participation in the life of the family\" is superior to institutionalization. The extent to which AFC is familial has been widely debated, ranging from claims that this environment provides a surrogate family, to claims that it is no more than a mini-institution. This paper discusses the extent to which elderly clients are integrated into the family and the method by which such integration was measured in a sample of one hundred adult foster homes in New York State. The four dimensions used to measure familism were Affection, Social Interaction, the performance of Ritual, and the minimization of Social Distance."}
{"id": "PMID:489140", "title": "Group progress of community elderly as measured by tape recordings, group tempo and group evaluation.", "content": "Community based geriatric outpatients (N:67) were involved in a 12-session group resocialization program conducted at a municipal hospital. Meetings were taped and records kept on all group interactions. Short-term behavioral changes in quality of group participation and evaluation of the group experience by the members are reported. Comparison is made between the two experimental groups led by a trained group leader who used group intervention techniques and two \"talk\" groups in which the leader played a non-intervention role. Results indicate that experimental group members showed changes toward more active problem-solving approaches over time and maintained a lively group tempo. \"Talk\" group members remained fixed at one level evidencing little change along with a depressed tempo during group sessions.", "contents": "Group progress of community elderly as measured by tape recordings, group tempo and group evaluation. Community based geriatric outpatients (N:67) were involved in a 12-session group resocialization program conducted at a municipal hospital. Meetings were taped and records kept on all group interactions. Short-term behavioral changes in quality of group participation and evaluation of the group experience by the members are reported. Comparison is made between the two experimental groups led by a trained group leader who used group intervention techniques and two \"talk\" groups in which the leader played a non-intervention role. Results indicate that experimental group members showed changes toward more active problem-solving approaches over time and maintained a lively group tempo. \"Talk\" group members remained fixed at one level evidencing little change along with a depressed tempo during group sessions."}
{"id": "PMID:489147", "title": "Analysis of ECG data, for data compression.", "content": "A number of papers on the subject of data reduction techniques applied to ECG Data have recently been published; however, the authors found that most of these articles did not consider quantization techniques, which can be effectively applied to ECG data without any complex parameter extraction procedures. In this paper the authors have looked at the effects of quantization on ECG data and techniques of reducing the amount of data needed to represent these signals. Basically, 3 data reduction techniques, linear prediction using differential pulse code modulation, spectral analysis and slope change detection are investigated and a relative assessment of their performance is presented. This analysis revealed that a slope change detection, as applied to prefiltered data, can be used to represent ECG data at a rate of 2 bits/sample, while maintaining the mean squared error and peak error below 1% and 5% respectively. This technique therefore gives an effective 3 to 1 reduction over the original sampled data, since it was found that the original data could be quantized to 6 bits without significant loss of waveform information.", "contents": "Analysis of ECG data, for data compression. A number of papers on the subject of data reduction techniques applied to ECG Data have recently been published; however, the authors found that most of these articles did not consider quantization techniques, which can be effectively applied to ECG data without any complex parameter extraction procedures. In this paper the authors have looked at the effects of quantization on ECG data and techniques of reducing the amount of data needed to represent these signals. Basically, 3 data reduction techniques, linear prediction using differential pulse code modulation, spectral analysis and slope change detection are investigated and a relative assessment of their performance is presented. This analysis revealed that a slope change detection, as applied to prefiltered data, can be used to represent ECG data at a rate of 2 bits/sample, while maintaining the mean squared error and peak error below 1% and 5% respectively. This technique therefore gives an effective 3 to 1 reduction over the original sampled data, since it was found that the original data could be quantized to 6 bits without significant loss of waveform information."}
{"id": "PMID:489141", "title": "Relationships between intelligence and concept identification in adulthood as a function of stage of learning.", "content": "Three groups (n = 54) of participants aged 17-26, 39-51 and 59-76 were asked to solve two types of concept problems to investigate the intellectual correlates of concept identification as a function of stage of learning in adulthood. Predictions derived from the Gf-Gc theory of Horn and Cattell were partially supported in each age group. Differential ability-performance relations as a function of stage of learning were considerably less potent in the elderly versus the young and middle aged. Comparisons both within and between age groups suggested that a dynamic picture of interactions among organismic and task-related variables is preferable, reflecting structural changes in such relationships with practice and cohort membership.", "contents": "Relationships between intelligence and concept identification in adulthood as a function of stage of learning. Three groups (n = 54) of participants aged 17-26, 39-51 and 59-76 were asked to solve two types of concept problems to investigate the intellectual correlates of concept identification as a function of stage of learning in adulthood. Predictions derived from the Gf-Gc theory of Horn and Cattell were partially supported in each age group. Differential ability-performance relations as a function of stage of learning were considerably less potent in the elderly versus the young and middle aged. Comparisons both within and between age groups suggested that a dynamic picture of interactions among organismic and task-related variables is preferable, reflecting structural changes in such relationships with practice and cohort membership."}
{"id": "PMID:489142", "title": "Correlates of life satisfaction: an aid analysis.", "content": "The Automatic Interaction Detector (AID3) was used to develop a model based on the interaction of predictors of life satisfaction. The sample consisted of 871 people over fifty-five years of age. Thirty-one potential predictors were used representing demographics, environmental variables, and social psychological variables. The findings indicate that nine predictors explained 22.1% of the variance in life satisfaction scores. The most important predictors of life satisfaction were family life satisfaction, personal health satisfaction, and satisfaction with dwelling. The interactions between the predictors indicated that a simple linear-monotonic relationship between the predictors was too restrictive.", "contents": "Correlates of life satisfaction: an aid analysis. The Automatic Interaction Detector (AID3) was used to develop a model based on the interaction of predictors of life satisfaction. The sample consisted of 871 people over fifty-five years of age. Thirty-one potential predictors were used representing demographics, environmental variables, and social psychological variables. The findings indicate that nine predictors explained 22.1% of the variance in life satisfaction scores. The most important predictors of life satisfaction were family life satisfaction, personal health satisfaction, and satisfaction with dwelling. The interactions between the predictors indicated that a simple linear-monotonic relationship between the predictors was too restrictive."}
{"id": "PMID:489148", "title": "A real-time programming system.", "content": "The paper describes a Basic Operating and Scheduling System (BOSS) designed for a small computer. User programs are organised as self-contained modular 'processes' and the way in which the scheduler divides the time of the computer equally between them, while arranging for any process which has to respond to an interrupt from a peripheral device to be given the necessary priority, is described in detail. Next the procedures provided by the operating system to organise communication between processes are described, and how they are used to construct dynamically self-modifying real-time systems. Finally, the general philosophy of BOSS and applications to a multi-processor assembly are discussed.", "contents": "A real-time programming system. The paper describes a Basic Operating and Scheduling System (BOSS) designed for a small computer. User programs are organised as self-contained modular 'processes' and the way in which the scheduler divides the time of the computer equally between them, while arranging for any process which has to respond to an interrupt from a peripheral device to be given the necessary priority, is described in detail. Next the procedures provided by the operating system to organise communication between processes are described, and how they are used to construct dynamically self-modifying real-time systems. Finally, the general philosophy of BOSS and applications to a multi-processor assembly are discussed."}
{"id": "PMID:489149", "title": "Computer simulation as a tool for clinical trial design.", "content": "Use of a computer simulation technique as a tool for optimising the design of a clinical trial is described. One of several aspects of trial design facilitated by use of the technique is the estimation of the number of patients required in the trial. The simulation tool provides the basis for structured and disciplined discussion of all the issues to be considered in designing a trial. Copies of the simulation program are available from the author.", "contents": "Computer simulation as a tool for clinical trial design. Use of a computer simulation technique as a tool for optimising the design of a clinical trial is described. One of several aspects of trial design facilitated by use of the technique is the estimation of the number of patients required in the trial. The simulation tool provides the basis for structured and disciplined discussion of all the issues to be considered in designing a trial. Copies of the simulation program are available from the author."}
{"id": "PMID:489150", "title": "[Comparison of algorithms for the resolution of a secular equation in the vibrational analysis of biological molecules].", "content": "A computer analysis of several solution methods for secular equations leads to a determination of their optimum conditions. The results contribute to the application of a new preliminary method of frequency assignment to the vibrational study of the heavy atoms coordination sphere in complex biological structures, such as metalporphyrins and adenosine triphosphate.", "contents": "[Comparison of algorithms for the resolution of a secular equation in the vibrational analysis of biological molecules]. A computer analysis of several solution methods for secular equations leads to a determination of their optimum conditions. The results contribute to the application of a new preliminary method of frequency assignment to the vibrational study of the heavy atoms coordination sphere in complex biological structures, such as metalporphyrins and adenosine triphosphate."}
{"id": "PMID:489151", "title": "Model of hospital's inflow.", "content": "To be accepted as an inpatient in the Geneva Hospital one must proceed through a registration post. Data collecting sessions were set up there to study its working. Since many random components enter the registration procedure a stochastic queuing process is the appropriate model. The process main characteristics are determined by rather involved statistical and mathematical means. The values reckoned for the most relevant parameters (to both medical staff and general public) are found quite plausible.", "contents": "Model of hospital's inflow. To be accepted as an inpatient in the Geneva Hospital one must proceed through a registration post. Data collecting sessions were set up there to study its working. Since many random components enter the registration procedure a stochastic queuing process is the appropriate model. The process main characteristics are determined by rather involved statistical and mathematical means. The values reckoned for the most relevant parameters (to both medical staff and general public) are found quite plausible."}
{"id": "PMID:489152", "title": "Toward adaptive (computer-based) hospital care systems that can grow and improve as a function of user participation.", "content": "This paper describes a computer-based system that would allow doctors, patients, nurses, researchers and experts to participate in medical care in ways that will enhance the usefulness of the system, and will allow the system to grow, adapt and improve as a function of this participation.", "contents": "Toward adaptive (computer-based) hospital care systems that can grow and improve as a function of user participation. This paper describes a computer-based system that would allow doctors, patients, nurses, researchers and experts to participate in medical care in ways that will enhance the usefulness of the system, and will allow the system to grow, adapt and improve as a function of this participation."}
{"id": "PMID:489153", "title": "[Research method using discriminant subsets on minicomputers].", "content": "Given a set of elements (illnesses, medicines,...) characterised by criteria or attributes (symptoms, characteristics, colour,...), each able to assume a certain number of states (for colour, more than nine), the problem is to find a minimal discriminant chain, or in other words, the smallest sequence of criteria enabling one to distinguish the elements of the initial set, unless of course they are identical. This problem is very general, but when tackled together it often involves excessive calculations. Based on the research principles proposed by Begon and Tremolieres, the authors propose a method well adapted to criteria in several states, which can be used on a small computer, when searching for pseudo-minimal chains by sampling. An application of this method (in liaison with the 'Centre Anti-Poisons', Nancy Pr Larcan) has enabled us to carry out a search for discriminant chains for more than 2800 medical tablets by their external characteristics, among which 3 quantitative criteria were known with a certain margin of error, and 21 qualitative criteria, making a total of more than 60 different states.", "contents": "[Research method using discriminant subsets on minicomputers]. Given a set of elements (illnesses, medicines,...) characterised by criteria or attributes (symptoms, characteristics, colour,...), each able to assume a certain number of states (for colour, more than nine), the problem is to find a minimal discriminant chain, or in other words, the smallest sequence of criteria enabling one to distinguish the elements of the initial set, unless of course they are identical. This problem is very general, but when tackled together it often involves excessive calculations. Based on the research principles proposed by Begon and Tremolieres, the authors propose a method well adapted to criteria in several states, which can be used on a small computer, when searching for pseudo-minimal chains by sampling. An application of this method (in liaison with the 'Centre Anti-Poisons', Nancy Pr Larcan) has enabled us to carry out a search for discriminant chains for more than 2800 medical tablets by their external characteristics, among which 3 quantitative criteria were known with a certain margin of error, and 21 qualitative criteria, making a total of more than 60 different states."}
{"id": "PMID:489154", "title": "Computer programs to implement retinal models.", "content": "To implement retinal models of some complexity a set of computer programs, coordinated by a main program is required. A set of said programs is presented here which permits the design and the experimentation with linear and non-linear retinal layered models. A main program, called RETINA, is the basis for the building of a model, the generation of the input stimuli and the experimentation with the overall system.", "contents": "Computer programs to implement retinal models. To implement retinal models of some complexity a set of computer programs, coordinated by a main program is required. A set of said programs is presented here which permits the design and the experimentation with linear and non-linear retinal layered models. A main program, called RETINA, is the basis for the building of a model, the generation of the input stimuli and the experimentation with the overall system."}
{"id": "PMID:489155", "title": "A theoretical model for layered visual processing.", "content": "A theoretical general model of layered computation in the retina is presented. Each layer is functional in the sense that it may correspond or not to an anatomical layer. It is formed by computing elements which can perform in principle any non-linear arbitrary function on a three-dimensional input space. This space consists of 2 spatial dimensions, plus time. The function performed by each computing element of a layer is simplified for the cases of invariance, space or time linearity and for time independence. Two illustrations are also presented. The first is a model of the simple ganglion cells in cat's retina. The second, a model of the group 2 ganglion cell of the frog's retina.", "contents": "A theoretical model for layered visual processing. A theoretical general model of layered computation in the retina is presented. Each layer is functional in the sense that it may correspond or not to an anatomical layer. It is formed by computing elements which can perform in principle any non-linear arbitrary function on a three-dimensional input space. This space consists of 2 spatial dimensions, plus time. The function performed by each computing element of a layer is simplified for the cases of invariance, space or time linearity and for time independence. Two illustrations are also presented. The first is a model of the simple ganglion cells in cat's retina. The second, a model of the group 2 ganglion cell of the frog's retina."}
{"id": "PMID:489156", "title": "Multicompartment pharmacokinetic analysis and simulations using a programmable calculator.", "content": "This paper describes the use of a programmable calculator (HP-97), for fitting plasma concentration profiles to 1 and 2 compartment open models and calculating fitted and derived parameters together with estimates of steady state plasma drug levels following various dosage regimens in clinical settings.", "contents": "Multicompartment pharmacokinetic analysis and simulations using a programmable calculator. This paper describes the use of a programmable calculator (HP-97), for fitting plasma concentration profiles to 1 and 2 compartment open models and calculating fitted and derived parameters together with estimates of steady state plasma drug levels following various dosage regimens in clinical settings."}
{"id": "PMID:489157", "title": "Digital filtering of on-line evoked potentials.", "content": "Evoked potentials recorded automatically at frequent intervals are a useful adjunct for monitoring head injury patients; however, unaveraged residual noise due to patient movement and synchronization of th- stimulus to harmonics of the line frequency is sometimes present. A frequency analysis was performed on 23 records with varying degrees of unaveraged noise and the results were used to design a digital filter. The frequency content of the records analyzed was largely contained in a band from D.C. to 200 Hz. A zero phase shift lowpass digital filter with a cutoff frequency of 200 Hz eliminated the majority of noise. Despite the low frequency content, a wide-band amplifier is still required to avoid waveform distortion, particularly latencies. Digital filters can then be applied without causing distortion.", "contents": "Digital filtering of on-line evoked potentials. Evoked potentials recorded automatically at frequent intervals are a useful adjunct for monitoring head injury patients; however, unaveraged residual noise due to patient movement and synchronization of th- stimulus to harmonics of the line frequency is sometimes present. A frequency analysis was performed on 23 records with varying degrees of unaveraged noise and the results were used to design a digital filter. The frequency content of the records analyzed was largely contained in a band from D.C. to 200 Hz. A zero phase shift lowpass digital filter with a cutoff frequency of 200 Hz eliminated the majority of noise. Despite the low frequency content, a wide-band amplifier is still required to avoid waveform distortion, particularly latencies. Digital filters can then be applied without causing distortion."}
{"id": "PMID:489158", "title": "[Original method of multivariate analysis, adapted for comparison of mean evoked potentials: E2 test].", "content": "This paper describes a multivariate statistical method of analysis for the comparison of 2 paired series. For such paired series, this test, called E2, is analogous to the generalized Hotteling T2 test, as epsilon test (pairing method) is analogous to Student's t test. Here we give an example of its application to a comparison of curves, in this particular case average auditory evoked potentials, picked up at symmetrical points on the scalp.", "contents": "[Original method of multivariate analysis, adapted for comparison of mean evoked potentials: E2 test]. This paper describes a multivariate statistical method of analysis for the comparison of 2 paired series. For such paired series, this test, called E2, is analogous to the generalized Hotteling T2 test, as epsilon test (pairing method) is analogous to Student's t test. Here we give an example of its application to a comparison of curves, in this particular case average auditory evoked potentials, picked up at symmetrical points on the scalp."}
{"id": "PMID:489159", "title": "Application of optimized parallel processing digital computers and numerical approximation methods to the ultra high-speed three-dimensional reconstruction of the intact thorax.", "content": "In order to achieve the computational capability to carry out many thousands of cross-sectional reconstructions, necessary to support a prototype high temporal and spatial resolution cylindrical scanning multiaxial tomographic unit, a series of design, software simulation, and fabrication studies is underway to develop a special-purpose high-speed reconstruction computer. This processor will rely upon integrated circuit arithmetic components of advanced design, and highly parallel architecture to execute X-ray based transaxial reconstruction algorithms at the rate of hundreds of cross sections/sec.", "contents": "Application of optimized parallel processing digital computers and numerical approximation methods to the ultra high-speed three-dimensional reconstruction of the intact thorax. In order to achieve the computational capability to carry out many thousands of cross-sectional reconstructions, necessary to support a prototype high temporal and spatial resolution cylindrical scanning multiaxial tomographic unit, a series of design, software simulation, and fabrication studies is underway to develop a special-purpose high-speed reconstruction computer. This processor will rely upon integrated circuit arithmetic components of advanced design, and highly parallel architecture to execute X-ray based transaxial reconstruction algorithms at the rate of hundreds of cross sections/sec."}
{"id": "PMID:489160", "title": "A non-linear mathematical model for the in vivo determination of Kupffer cells number and rate of phagocytosis of radiocolloids in rats.", "content": "In order to perform a quantification of the hepatic RES function, a mathematical model of the colloids phagocytosis was constructed and validated in normal and partially hepatectomised rats. The experimental design consisted of the collection of successive blood samples for the measurement of radiocolloids time courses after the injection of different doses of gelatin colloids (0.075--5 mg of gelatin/100 g body wt). The unknown parameters were estimated by method of maximum likelihood using a second order algorithm. A good fit between experimental and simulated data was obtained for a large range of injected doses using a single set of parameters. Comparisons of parameters values between normal and hepatectomised rats were found to be -onsistent with the hepatectomy ratio. This computerised estimation of parameters provides a determination of both the total number of Kupffer cells and the mean time of a complete phagocytosis cycle which cannot be obtained by classical approaches.", "contents": "A non-linear mathematical model for the in vivo determination of Kupffer cells number and rate of phagocytosis of radiocolloids in rats. In order to perform a quantification of the hepatic RES function, a mathematical model of the colloids phagocytosis was constructed and validated in normal and partially hepatectomised rats. The experimental design consisted of the collection of successive blood samples for the measurement of radiocolloids time courses after the injection of different doses of gelatin colloids (0.075--5 mg of gelatin/100 g body wt). The unknown parameters were estimated by method of maximum likelihood using a second order algorithm. A good fit between experimental and simulated data was obtained for a large range of injected doses using a single set of parameters. Comparisons of parameters values between normal and hepatectomised rats were found to be -onsistent with the hepatectomy ratio. This computerised estimation of parameters provides a determination of both the total number of Kupffer cells and the mean time of a complete phagocytosis cycle which cannot be obtained by classical approaches."}
{"id": "PMID:489161", "title": "Large interindividual variations in metabolism of benzo(alpha)pyrene by peripheral lung tissue from lung cancer patients.", "content": "A very large variation (44-fold) was observed in the ability of short-term organ cultures of peripheral lung tissue from lung-cancer patients to metabolize the environmental carcinogen benzo(alpha)pyrene to organic solvent-soluble metabolites. The amounts of benzo(alpha)pyrene (2 microM) metabolized ranged from little (1%) to almost total (96.2%) metabolism within 24 h of culture. Previous work by Kellerman et al. (1973) has suggested a relationship between susceptibility to lung cancer and the indicibility of aryl hydrocarbon hydroxylase activity in cultured human lymphocytes. The metabolic fate of carcinogenic polycyclic aromatic hydrocarbons in the respiratory tract in vivo is undoubtedly more closely mimicked by short-term organ culture of human lung than by cultured lymphocytes. Thus the very wide interindividual variation observed in pulmonary metabolism of benzo(alpha)pyrene in this study and the large variations in covalent binding to human bronchial DNA observed by Harris et al. (1976) strongly suggest that there may be little basis for screening humans for variations in lymphocyte aryl hydrocarbon hydroxylase activity as a means of assessing their susceptibility to lung cancer.", "contents": "Large interindividual variations in metabolism of benzo(alpha)pyrene by peripheral lung tissue from lung cancer patients. A very large variation (44-fold) was observed in the ability of short-term organ cultures of peripheral lung tissue from lung-cancer patients to metabolize the environmental carcinogen benzo(alpha)pyrene to organic solvent-soluble metabolites. The amounts of benzo(alpha)pyrene (2 microM) metabolized ranged from little (1%) to almost total (96.2%) metabolism within 24 h of culture. Previous work by Kellerman et al. (1973) has suggested a relationship between susceptibility to lung cancer and the indicibility of aryl hydrocarbon hydroxylase activity in cultured human lymphocytes. The metabolic fate of carcinogenic polycyclic aromatic hydrocarbons in the respiratory tract in vivo is undoubtedly more closely mimicked by short-term organ culture of human lung than by cultured lymphocytes. Thus the very wide interindividual variation observed in pulmonary metabolism of benzo(alpha)pyrene in this study and the large variations in covalent binding to human bronchial DNA observed by Harris et al. (1976) strongly suggest that there may be little basis for screening humans for variations in lymphocyte aryl hydrocarbon hydroxylase activity as a means of assessing their susceptibility to lung cancer."}
{"id": "PMID:489162", "title": "Effect of parity regimen on the rate of occurrence of mammary tumors in A, C3H, and RIII mice.", "content": "The occurrence rates of mammary tumors as affected by breeding regimen (early in life, late, continuous, or not at all) in A, RIII, and C3H mice were observed. The response to the breeding regimen was different in each of the three strains. The C3H stock was affected least, although the tumor occurrence rate was slower in virgins. In both A and RIII, only one litter at puberty resulted in the tumors occurring over the greatest age range; and in RIII mice, the occurrence rate and the mean tumor age were similar to those of the virgins. Normal continuous breeding caused the earliest tumors in all three strains, although the RIII mice, breeding after 18 weeks of age also caused very early tumors. The response of RIII strain to parity variations was more like that of humans than was the response of either of the other strains. Removal of the milk-transmitted virus from these strains by foster-nursing resulted in vastly different mammary tumor occurrence rates, the quantitative changes being different in each mouse strain.", "contents": "Effect of parity regimen on the rate of occurrence of mammary tumors in A, C3H, and RIII mice. The occurrence rates of mammary tumors as affected by breeding regimen (early in life, late, continuous, or not at all) in A, RIII, and C3H mice were observed. The response to the breeding regimen was different in each of the three strains. The C3H stock was affected least, although the tumor occurrence rate was slower in virgins. In both A and RIII, only one litter at puberty resulted in the tumors occurring over the greatest age range; and in RIII mice, the occurrence rate and the mean tumor age were similar to those of the virgins. Normal continuous breeding caused the earliest tumors in all three strains, although the RIII mice, breeding after 18 weeks of age also caused very early tumors. The response of RIII strain to parity variations was more like that of humans than was the response of either of the other strains. Removal of the milk-transmitted virus from these strains by foster-nursing resulted in vastly different mammary tumor occurrence rates, the quantitative changes being different in each mouse strain."}
{"id": "PMID:489163", "title": "A quantitative comparison of the mutagenicity of carcinogenic polycyclic hydrocarbon derivatives in cultured mammalian cells.", "content": "The mutagenicity of a series of reactive polycyclic hydrocarbon derivatives has been studied using Chinese hamster (V79) cells in culture and, as a mutational marker, resistance to the purine analogue 8-azaguanine. The compounds were compared by relating mutation frequency to the dose applied (mutagenic effectiveness) to induced cytotoxicity (mutagenic efficiency) and to the extent of reaction of the hydrocarbon with DNA (absolute mutagenic efficiency). In each case anti-benzo(alpha)pyrene (BP)-7,8 dihydrodiol-9,10 oxide, the suspected ultimate carcinogenic form of benzo(alpha)pyrene, was by far the most potent of the compounds tested. Furthermore, the mutagenicity of the syn- and anti-BP-diolepoxide isomers correlated positively with their documenrences in the ability of each derivative to form a carbonium ion. Variations in mutagenic efficiency and absolute mutagenic efficiency were more difficult to explain. The latter findings are discussed in relation to the types of hydrocarbon-DNA product obtained with each compound and also to the possibility of a variable cellular response to more subtle differences in the chemistry of the hydrocarbon-DNA interaction.", "contents": "A quantitative comparison of the mutagenicity of carcinogenic polycyclic hydrocarbon derivatives in cultured mammalian cells. The mutagenicity of a series of reactive polycyclic hydrocarbon derivatives has been studied using Chinese hamster (V79) cells in culture and, as a mutational marker, resistance to the purine analogue 8-azaguanine. The compounds were compared by relating mutation frequency to the dose applied (mutagenic effectiveness) to induced cytotoxicity (mutagenic efficiency) and to the extent of reaction of the hydrocarbon with DNA (absolute mutagenic efficiency). In each case anti-benzo(alpha)pyrene (BP)-7,8 dihydrodiol-9,10 oxide, the suspected ultimate carcinogenic form of benzo(alpha)pyrene, was by far the most potent of the compounds tested. Furthermore, the mutagenicity of the syn- and anti-BP-diolepoxide isomers correlated positively with their documenrences in the ability of each derivative to form a carbonium ion. Variations in mutagenic efficiency and absolute mutagenic efficiency were more difficult to explain. The latter findings are discussed in relation to the types of hydrocarbon-DNA product obtained with each compound and also to the possibility of a variable cellular response to more subtle differences in the chemistry of the hydrocarbon-DNA interaction."}
{"id": "PMID:489164", "title": "Importance of a collagen substratum for stimulation of capillary endothelial cell proliferation by tumour angiogenesis factor.", "content": "Tumour extracts were obtained from rat Walker 256 carcinoma and examined for the presence of tumour angiogenesis factor (TAF) in vivo before being used in tissue culture experiments. Capillary endothelial cells derived from cow brain white matter were used to study the effects of TAF-containing tumour extracts on cell proliferation in vitro. The cells were grown on two types of substrata: (1) plastic tissue culture dishes and (2) hydrated gels made of rat tail tendon type I collagen. Human platelets or platelet-released factors were introduced into the system because of the many inter-relationships known to exist between platelets, collagen and endothelial cells. If trypsin was used during the preparation of TAT, the resulting batches stimulated endothelial cell proliferation only when the cells were growing on a collagen substratum and either platelets or platelet-released factors were present in the growth medium. If incubation with trypsin was omitted from the TAF extraction procedure, the resulting batches stimulated cell growth both on plastic and on collagen. A synergistic interaction also occurred between these TAF-containing tumour extracts and platelet-released factors. This effect was always more marked when the cells were growing on collagen than when on plastic. These data suggest that the nature of the substratum affects the response of the endothelial cells to TAF and to platelet-released factors.", "contents": "Importance of a collagen substratum for stimulation of capillary endothelial cell proliferation by tumour angiogenesis factor. Tumour extracts were obtained from rat Walker 256 carcinoma and examined for the presence of tumour angiogenesis factor (TAF) in vivo before being used in tissue culture experiments. Capillary endothelial cells derived from cow brain white matter were used to study the effects of TAF-containing tumour extracts on cell proliferation in vitro. The cells were grown on two types of substrata: (1) plastic tissue culture dishes and (2) hydrated gels made of rat tail tendon type I collagen. Human platelets or platelet-released factors were introduced into the system because of the many inter-relationships known to exist between platelets, collagen and endothelial cells. If trypsin was used during the preparation of TAT, the resulting batches stimulated endothelial cell proliferation only when the cells were growing on a collagen substratum and either platelets or platelet-released factors were present in the growth medium. If incubation with trypsin was omitted from the TAF extraction procedure, the resulting batches stimulated cell growth both on plastic and on collagen. A synergistic interaction also occurred between these TAF-containing tumour extracts and platelet-released factors. This effect was always more marked when the cells were growing on collagen than when on plastic. These data suggest that the nature of the substratum affects the response of the endothelial cells to TAF and to platelet-released factors."}
{"id": "PMID:489165", "title": "Augmentation of cell-mediated cytotoxicity to a rat lymphoma. I. Stimulation of non-T-cell cytotoxicity in vivo by tumour cells.", "content": "The injection of a syngeneic Gross-virus-induced lymphoma into W/Fu rats induced peaks of cytotoxicity in the spleen attributable to non-T cells and T cells 3 and 10 days later, respectively. The conditions required for augmenting the cytotoxicity of the non-T cells in various lymphoid compartments (shown elsewhere to closely resemble NK cells) were analysed using the ip and iv routes of inoculation and a variety of tumour cells including those normally susceptible or resistant to lysis by NK cells in vitro. Using an ip inoculation of W/FuG-1 cells (a tumour susceptible to lysis by NK cells), a short-lived, 3-fold increase in cytotoxicity was observed in the spleen at day 3 and a 5-fold increase in the PEC at day 5. Cytotoxicity in other lymphoid organs remained unchanged. Tumours resistant to lysis by NK cells also stimulated cytotoxicity in the spleen or PEC, although the effect depended on the dose and route of inoculation used, and depression of cytotoxicity was observed under some conditions.", "contents": "Augmentation of cell-mediated cytotoxicity to a rat lymphoma. I. Stimulation of non-T-cell cytotoxicity in vivo by tumour cells. The injection of a syngeneic Gross-virus-induced lymphoma into W/Fu rats induced peaks of cytotoxicity in the spleen attributable to non-T cells and T cells 3 and 10 days later, respectively. The conditions required for augmenting the cytotoxicity of the non-T cells in various lymphoid compartments (shown elsewhere to closely resemble NK cells) were analysed using the ip and iv routes of inoculation and a variety of tumour cells including those normally susceptible or resistant to lysis by NK cells in vitro. Using an ip inoculation of W/FuG-1 cells (a tumour susceptible to lysis by NK cells), a short-lived, 3-fold increase in cytotoxicity was observed in the spleen at day 3 and a 5-fold increase in the PEC at day 5. Cytotoxicity in other lymphoid organs remained unchanged. Tumours resistant to lysis by NK cells also stimulated cytotoxicity in the spleen or PEC, although the effect depended on the dose and route of inoculation used, and depression of cytotoxicity was observed under some conditions."}
{"id": "PMID:489167", "title": "Decreased sensitivity to interferon associated with in vitro transformation of X-ray-transformed C3H/10T1/2 cells.", "content": "We have compared the interferon sensitivity of parental 10T1/2 cells, having a phenotype characteristic of normal cells, with that of transformed cells isolated after X-irradiation of treatment with methylcholanthrene. Transformed cells were 10- to 20-fold less sensitive than the parental untransformed cells to the antiviral action of interferon and were also less sensitive to the inhibitory effect of interferon on cell multiplication. The decreased sensitivity of these cells to interferon is a useful marker of the transformed phenotype.", "contents": "Decreased sensitivity to interferon associated with in vitro transformation of X-ray-transformed C3H/10T1/2 cells. We have compared the interferon sensitivity of parental 10T1/2 cells, having a phenotype characteristic of normal cells, with that of transformed cells isolated after X-irradiation of treatment with methylcholanthrene. Transformed cells were 10- to 20-fold less sensitive than the parental untransformed cells to the antiviral action of interferon and were also less sensitive to the inhibitory effect of interferon on cell multiplication. The decreased sensitivity of these cells to interferon is a useful marker of the transformed phenotype."}
{"id": "PMID:489168", "title": "Chromosome breaking activity of human feces and its enhancement by transition metals.", "content": "Chloroform-methanol extracts from human fecal samples were found to contain compounds which induce chromosome aberrations in Chinese hamster ovary cells. The induction of chromosome aberrations is stimulated by Cu2+ or Mn2+ and inhibited by Fe2+ or Fe3+. Addition of catalase to the fecal extract or the mixture of fecal extract and Mn2+ reduced the frequency of chromosome aberrations. These properties are indicative of hydroxyradical producing agents.", "contents": "Chromosome breaking activity of human feces and its enhancement by transition metals. Chloroform-methanol extracts from human fecal samples were found to contain compounds which induce chromosome aberrations in Chinese hamster ovary cells. The induction of chromosome aberrations is stimulated by Cu2+ or Mn2+ and inhibited by Fe2+ or Fe3+. Addition of catalase to the fecal extract or the mixture of fecal extract and Mn2+ reduced the frequency of chromosome aberrations. These properties are indicative of hydroxyradical producing agents."}
{"id": "PMID:489169", "title": "Induction of reproductive system tumors in mice by N6-(methylnitroso)-adenosine and a tumorigenic effect of its combined precursors.", "content": "Interaction of the naturally-occurring nucleoside, N6-methyl adenosine, with nitrite, a reaction that occurs readily under acidic conditions, results in the formation of a nitrosamine, N6-(methylnitroso) adenosine[m6(NO)Ado]. This nitrosamine was given in the drinking water (1 mM solution) of non-inbred Swiss mice from 3 weeks of age until death. It caused a significant increase in the incidence of primary lung tumors, compared with controls. It also induced reproductive tract tumors in 80% of the exposed females, including mammary tumors in 60% and uterine tumors in 25%. The precursors of m6(NO) Ado, m6Ado and nitrite, did not elevate tumor incidence when given singly, but when administered together resulted in a significant increase in numbers of lung tumors in the males. The nitrosamine base, N6-(methylnitroso)adenine, was found to be a less potent carcinogen than m6(NO)Ado, causing lung tumors only in males and possibly a few mammary tumors in females. These results indicate the in vivo formation of a carcinogen from m6Ado and nitrite, and show that m6(NO)Ado induces neoplasms in the reproductive system of mice, an unusual target for a N-nitroso carcinogen.", "contents": "Induction of reproductive system tumors in mice by N6-(methylnitroso)-adenosine and a tumorigenic effect of its combined precursors. Interaction of the naturally-occurring nucleoside, N6-methyl adenosine, with nitrite, a reaction that occurs readily under acidic conditions, results in the formation of a nitrosamine, N6-(methylnitroso) adenosine[m6(NO)Ado]. This nitrosamine was given in the drinking water (1 mM solution) of non-inbred Swiss mice from 3 weeks of age until death. It caused a significant increase in the incidence of primary lung tumors, compared with controls. It also induced reproductive tract tumors in 80% of the exposed females, including mammary tumors in 60% and uterine tumors in 25%. The precursors of m6(NO) Ado, m6Ado and nitrite, did not elevate tumor incidence when given singly, but when administered together resulted in a significant increase in numbers of lung tumors in the males. The nitrosamine base, N6-(methylnitroso)adenine, was found to be a less potent carcinogen than m6(NO)Ado, causing lung tumors only in males and possibly a few mammary tumors in females. These results indicate the in vivo formation of a carcinogen from m6Ado and nitrite, and show that m6(NO)Ado induces neoplasms in the reproductive system of mice, an unusual target for a N-nitroso carcinogen."}
{"id": "PMID:489170", "title": "Transplacental mutagenesis of products formed in the stomach of golden hamsters given sodium nitrite and morpholine.", "content": "Hamster embryos were exposed in utero to the action of sodium nitrite (NaNO2) and morpholine (Mo) administered simultaneously by stomach tube to the mothers on the 11th or 12th day of pregnancy. Embryo cells were examined for chromosomal aberrations, micronuclear formation, morphological or malignant transformation and drug resistance mutations. For detection of induced mutations, the embryo cells were cultured in normal medium for 72 h and then transferred to medium containing 10 or 20 micrograms/ml of 8-azaguanine (8AG) or 1m7 ouabain (Oua). The number of 8AG-, Ouaresistant colonies was markedly increased after administration of NaNO2 and Mo. The embryonic fibroblasts also showed a markedly increased frequency of micronucleation and a slight increase in chromosome aberrations. This treatment also caused morphological or malignant transformation of fetal cells. After cultivation in vitro, cells from some transformed colonies produced tumors when inoculated into the cheek pouch of young golden hamsters. Orally administered N-nitroso-morpholine (N-Mo), as a positive control, had the same transplacental biological actions on embryonic fibroblasts. However, transplacentally Mo alone was ineffective. A single administration of 500 mg/kg NaNO2 had only slight biological effects. N-Mo was produced in the stomachs of animals treated simultaneously with NaNO2 and Mo. A small amount of a nitrosamine, N-nitrosodimethylamine (DMN), was detected in the stomach after a single dose of NaNO2.", "contents": "Transplacental mutagenesis of products formed in the stomach of golden hamsters given sodium nitrite and morpholine. Hamster embryos were exposed in utero to the action of sodium nitrite (NaNO2) and morpholine (Mo) administered simultaneously by stomach tube to the mothers on the 11th or 12th day of pregnancy. Embryo cells were examined for chromosomal aberrations, micronuclear formation, morphological or malignant transformation and drug resistance mutations. For detection of induced mutations, the embryo cells were cultured in normal medium for 72 h and then transferred to medium containing 10 or 20 micrograms/ml of 8-azaguanine (8AG) or 1m7 ouabain (Oua). The number of 8AG-, Ouaresistant colonies was markedly increased after administration of NaNO2 and Mo. The embryonic fibroblasts also showed a markedly increased frequency of micronucleation and a slight increase in chromosome aberrations. This treatment also caused morphological or malignant transformation of fetal cells. After cultivation in vitro, cells from some transformed colonies produced tumors when inoculated into the cheek pouch of young golden hamsters. Orally administered N-nitroso-morpholine (N-Mo), as a positive control, had the same transplacental biological actions on embryonic fibroblasts. However, transplacentally Mo alone was ineffective. A single administration of 500 mg/kg NaNO2 had only slight biological effects. N-Mo was produced in the stomachs of animals treated simultaneously with NaNO2 and Mo. A small amount of a nitrosamine, N-nitrosodimethylamine (DMN), was detected in the stomach after a single dose of NaNO2."}
{"id": "PMID:489171", "title": "A review of psychoactive drug-involved deaths in nine major United States cities.", "content": "Detailed psychosocial and biomedical data were collected in 2,000 psychoactive drug-involved deaths occurring from 1972 through 1974 in nine large cities in the United States. The cases were selected representatively by the medical examiners or coroners in each city. Also, proficiency studies were carried out of the toxicological laboratories associated with these nine cooperating data collection centers. There were striking intercity psychosocial and biomedical differences in these psychoactive drug-involved deaths. These differences were based not simply in demographic regional population differences but also on the kinds of psychoactive drugs used as well as the role of the drug in contributing to death and whether the death was a result of an accident, suicide, homicide, or unknown intent. Also, a lack of uniformity was demonstrated in the quality control of the toxicological laboratories associated with the offices of these nine medical examiners or coroners, which suggests varying degrees of accuracy in resulting medicolegal diagnoses. Hence national programs of drug abuse deterrence or prevention and treatment should deal specifically with the variety of psychoactive drug-involved deaths occurring in different urban areas rather than approaching these problems globally as if they were uniform and homogeneous, and our toxicological proficiency studies accentuate the importance of mandatory quality control studies for all toxicological laboratories in the United States.", "contents": "A review of psychoactive drug-involved deaths in nine major United States cities. Detailed psychosocial and biomedical data were collected in 2,000 psychoactive drug-involved deaths occurring from 1972 through 1974 in nine large cities in the United States. The cases were selected representatively by the medical examiners or coroners in each city. Also, proficiency studies were carried out of the toxicological laboratories associated with these nine cooperating data collection centers. There were striking intercity psychosocial and biomedical differences in these psychoactive drug-involved deaths. These differences were based not simply in demographic regional population differences but also on the kinds of psychoactive drugs used as well as the role of the drug in contributing to death and whether the death was a result of an accident, suicide, homicide, or unknown intent. Also, a lack of uniformity was demonstrated in the quality control of the toxicological laboratories associated with the offices of these nine medical examiners or coroners, which suggests varying degrees of accuracy in resulting medicolegal diagnoses. Hence national programs of drug abuse deterrence or prevention and treatment should deal specifically with the variety of psychoactive drug-involved deaths occurring in different urban areas rather than approaching these problems globally as if they were uniform and homogeneous, and our toxicological proficiency studies accentuate the importance of mandatory quality control studies for all toxicological laboratories in the United States."}
{"id": "PMID:489172", "title": "Self-esteem patterns distinctive of groups of drug abusing and other dysfunctional adolescents.", "content": "Self-report measures on seven scales representing four broad processes of self-esteem were used to contrast three groups of drug abusers (pretreatment, in-treatment, and posttreatment), two other groups of institutionalized dysfunctional youth, and a \"normal\" public school sample, all from a population of White middle-class suburban adolescents. Multivariate analysis of covariance with adjustment for age and sex was used to compare the dysfunctional groups with the normal group. Discrimination analysis portrayed the dimensions of self-esteem separating all the groups. Pretreatment and in-treatment drug abusers had significantly lower scores on all seven measures of self-esteem; posttreatment abusers did not show these decrements, but reported greater ease in sharing feelings than normals did. The learning disabled adoles reported a lower sense of well being and greater ease in sharing feelings. These outcomes suggest that low self-esteem is not a general consequence of personal dysfunction per se, and may play a causal role in drug abuse.", "contents": "Self-esteem patterns distinctive of groups of drug abusing and other dysfunctional adolescents. Self-report measures on seven scales representing four broad processes of self-esteem were used to contrast three groups of drug abusers (pretreatment, in-treatment, and posttreatment), two other groups of institutionalized dysfunctional youth, and a \"normal\" public school sample, all from a population of White middle-class suburban adolescents. Multivariate analysis of covariance with adjustment for age and sex was used to compare the dysfunctional groups with the normal group. Discrimination analysis portrayed the dimensions of self-esteem separating all the groups. Pretreatment and in-treatment drug abusers had significantly lower scores on all seven measures of self-esteem; posttreatment abusers did not show these decrements, but reported greater ease in sharing feelings than normals did. The learning disabled adoles reported a lower sense of well being and greater ease in sharing feelings. These outcomes suggest that low self-esteem is not a general consequence of personal dysfunction per se, and may play a causal role in drug abuse."}
{"id": "PMID:489173", "title": "Blue Heaven Farms.", "content": "A therapeutic community for antisocial adolescents is discussed. The facility's tribulations before instituting a designed program, including a treatment plan for each client, and the results of such a program are described. Also discussed are the inherent problems of such facilities in our culture and the built-in-failure which seems inevitable.", "contents": "Blue Heaven Farms. A therapeutic community for antisocial adolescents is discussed. The facility's tribulations before instituting a designed program, including a treatment plan for each client, and the results of such a program are described. Also discussed are the inherent problems of such facilities in our culture and the built-in-failure which seems inevitable."}
{"id": "PMID:489174", "title": "Psychosis. A probable result of propranolol therapy.", "content": "beta-Adrenoceptor blocking agents are important agents in the management of many cardiovascular problems. Multiple neuropsychiatric adverse reactions have been noted with their use. This article reviews these reactions and describes the case of a suspected propranolol-induced psychosis.", "contents": "Psychosis. A probable result of propranolol therapy. beta-Adrenoceptor blocking agents are important agents in the management of many cardiovascular problems. Multiple neuropsychiatric adverse reactions have been noted with their use. This article reviews these reactions and describes the case of a suspected propranolol-induced psychosis."}
{"id": "PMID:489175", "title": "A social-learning approach to student marijuana use.", "content": "Three hundred seventy-two college students were administered a battery of questionnaires designed to assess the predictive power of four social variables (social support, perceived sanctions, availability, family models), and six psychological ones (locus of control, mortality-conscience guilt, personal meanings, functions for continuing use, belief in consequences, societal and non-societal means of valued goal attainment) with respect to marijuana use. Results support a social learning theory interpretation of marijuana use. Users have had pleasant experiences, believe that sanctions are minimal, and that marijuana is not harmful. Six predictor variables correlated +.76 with use.", "contents": "A social-learning approach to student marijuana use. Three hundred seventy-two college students were administered a battery of questionnaires designed to assess the predictive power of four social variables (social support, perceived sanctions, availability, family models), and six psychological ones (locus of control, mortality-conscience guilt, personal meanings, functions for continuing use, belief in consequences, societal and non-societal means of valued goal attainment) with respect to marijuana use. Results support a social learning theory interpretation of marijuana use. Users have had pleasant experiences, believe that sanctions are minimal, and that marijuana is not harmful. Six predictor variables correlated +.76 with use."}
{"id": "PMID:489176", "title": "Drug use in college students: a test of sociodemographic and reference group models of explanation.", "content": "This study examined the importance of reference group variables in the understanding of drug use in college students. Other studies have investigated the role of peer orientation, and this study further elaborates on the issue by specifically looking into parents and peers as reference groups for the students. This study supports the importance of reference group variables in understanding the students' use of marijuana and/or hashish. In addition, it shows that the sociodemographic variables cannot predict drug use behavior as well as the reference group variables can.", "contents": "Drug use in college students: a test of sociodemographic and reference group models of explanation. This study examined the importance of reference group variables in the understanding of drug use in college students. Other studies have investigated the role of peer orientation, and this study further elaborates on the issue by specifically looking into parents and peers as reference groups for the students. This study supports the importance of reference group variables in understanding the students' use of marijuana and/or hashish. In addition, it shows that the sociodemographic variables cannot predict drug use behavior as well as the reference group variables can."}
{"id": "PMID:489177", "title": "Self-evaluation, role satisfaction and anxiety in the woman alcoholic.", "content": "Compared with a matched group of 100 controls, 100 alcoholic women were found to have a significantly poorer self-concept, to view themselves as less personally competent, to experience greater anxiety, and to be less satisfied with themselves. The alcoholic and nonalcoholic women had similar concepts of other men and women and shared similar views of the roles of women in comtemporary society. The need for research comparing alcoholic women with matched female controls was stressed.", "contents": "Self-evaluation, role satisfaction and anxiety in the woman alcoholic. Compared with a matched group of 100 controls, 100 alcoholic women were found to have a significantly poorer self-concept, to view themselves as less personally competent, to experience greater anxiety, and to be less satisfied with themselves. The alcoholic and nonalcoholic women had similar concepts of other men and women and shared similar views of the roles of women in comtemporary society. The need for research comparing alcoholic women with matched female controls was stressed."}
{"id": "PMID:489178", "title": "Outreach redefined: the impact on staff attitudes of a family education project.", "content": "We decided to implement a family education program in our methadone maintenance clinic as a means of reaching out to family members. In the course of designing and implementing the program, we uncovered a variety of problems concerned with staff's perception of their roles and their attitudes toward parents. This paper discusses the growing awareness of these problems, which seem to be of significant generic importance to any agency involved in setting up a new program. We also discuss the effects of these problems, attempts at resolution, and suggest some general criteria for evaluation.", "contents": "Outreach redefined: the impact on staff attitudes of a family education project. We decided to implement a family education program in our methadone maintenance clinic as a means of reaching out to family members. In the course of designing and implementing the program, we uncovered a variety of problems concerned with staff's perception of their roles and their attitudes toward parents. This paper discusses the growing awareness of these problems, which seem to be of significant generic importance to any agency involved in setting up a new program. We also discuss the effects of these problems, attempts at resolution, and suggest some general criteria for evaluation."}
{"id": "PMID:489179", "title": "Treatment of alcoholics with logotherapy.", "content": "This study evaluates the results of group logoanalysis with selected hospitalized alcoholics using the Purpose in Life (PIL) Test as a before-and-after measure of therapeutic outcome in comparison with controls. Results suggest that closed-end logoanalysis groups are superior to open-end groups, and that both are superior to controls in improving the patient's sense of meaning and purpose in life as measured by the PIL.", "contents": "Treatment of alcoholics with logotherapy. This study evaluates the results of group logoanalysis with selected hospitalized alcoholics using the Purpose in Life (PIL) Test as a before-and-after measure of therapeutic outcome in comparison with controls. Results suggest that closed-end logoanalysis groups are superior to open-end groups, and that both are superior to controls in improving the patient's sense of meaning and purpose in life as measured by the PIL."}
{"id": "PMID:489180", "title": "Drug use among native American young adults.", "content": "Young adult Native American students at a postsecondary educational institute showed a very high level of use of all drugs when compared with other samples of college age youth. Alcohol, marijuana, and amphetamines were drugs of choice: 31% had tried inhalants although there was little present use; 10% were involved in a drug subculture, using multiple drugs relatively heavily. The authors' interpretation of these findings is either the institution attracts drug users or young Native Americans from relatively isolated environments have higher susceptibility to drugs when they enter an urban (nonreservation) culture.", "contents": "Drug use among native American young adults. Young adult Native American students at a postsecondary educational institute showed a very high level of use of all drugs when compared with other samples of college age youth. Alcohol, marijuana, and amphetamines were drugs of choice: 31% had tried inhalants although there was little present use; 10% were involved in a drug subculture, using multiple drugs relatively heavily. The authors' interpretation of these findings is either the institution attracts drug users or young Native Americans from relatively isolated environments have higher susceptibility to drugs when they enter an urban (nonreservation) culture."}
{"id": "PMID:489181", "title": "Anomie and drug use in high school students.", "content": "The use of alcohol, tobacco, and drugs as it relates to anomie was studied in 11th grade students. A questionnaire was administered to 1,314 11th grade students which included Srole's Anomie Scale as well as items pertaining to the usage of alcohol, tobacco, marijuana, cocaine, LSD, heroin, amphetamines, barbiturates, and methaqualone. The name of each drug was given along with the colloquial terms typically used to identify each drug. Substance users showed significantly higher anomie scores than nonusers on alcohol, tobacco, marijuana, LSD, amphetamines, and barbiturates. Although the results were not statistically significant for cocaine, heroin, or methaqualone, the differences between substance users and nonusers were all in the predicted direction.", "contents": "Anomie and drug use in high school students. The use of alcohol, tobacco, and drugs as it relates to anomie was studied in 11th grade students. A questionnaire was administered to 1,314 11th grade students which included Srole's Anomie Scale as well as items pertaining to the usage of alcohol, tobacco, marijuana, cocaine, LSD, heroin, amphetamines, barbiturates, and methaqualone. The name of each drug was given along with the colloquial terms typically used to identify each drug. Substance users showed significantly higher anomie scores than nonusers on alcohol, tobacco, marijuana, LSD, amphetamines, and barbiturates. Although the results were not statistically significant for cocaine, heroin, or methaqualone, the differences between substance users and nonusers were all in the predicted direction."}
{"id": "PMID:489182", "title": "Work and health in Mexico.", "content": "It is suggested that the relationship between work and health and disease traditionally has been analyzed in a limited way in that work has been considered only as a situational factor which puts workers in contact with environmental risks. It is proposed that work is an essential category for the understanding of disease, if one tries to study disease not as an individual biological phenomenon but as a social (and biological) phenomenon that occurs to the collectivity. Furthermore, it is suggested that the relation between disease and work should be analyzed in terms of the elements of the work process, understood as a technical and social process, and the capacity of different social groups to realize their interests. Reviewing the general trends of development of the work process in Mexico, it is shown that a transformation in the causes of death among men of working age has occurred during the last 20 years and there has been an increase in the number and the rate of work accidents and occupational diseases, despite a significant under-registration factor. Finally, the labor legislation on health and safety is contrasted with its actual implementation. This problem is interpreted in the context of traditional Mexican trade unionism. It is shown that trade unions which have become democratic and have gained their independence have struggled more vigorously on occupational health and safety issues.", "contents": "Work and health in Mexico. It is suggested that the relationship between work and health and disease traditionally has been analyzed in a limited way in that work has been considered only as a situational factor which puts workers in contact with environmental risks. It is proposed that work is an essential category for the understanding of disease, if one tries to study disease not as an individual biological phenomenon but as a social (and biological) phenomenon that occurs to the collectivity. Furthermore, it is suggested that the relation between disease and work should be analyzed in terms of the elements of the work process, understood as a technical and social process, and the capacity of different social groups to realize their interests. Reviewing the general trends of development of the work process in Mexico, it is shown that a transformation in the causes of death among men of working age has occurred during the last 20 years and there has been an increase in the number and the rate of work accidents and occupational diseases, despite a significant under-registration factor. Finally, the labor legislation on health and safety is contrasted with its actual implementation. This problem is interpreted in the context of traditional Mexican trade unionism. It is shown that trade unions which have become democratic and have gained their independence have struggled more vigorously on occupational health and safety issues."}
{"id": "PMID:489183", "title": "The export of hazardous factories to developing nations.", "content": "The export of hazardous industrial plants to developing nations is examined for a number of industries. As hazardous and polluting industries come under increasing regulation in industrial nations, some of the affected processes are exported, without improvements to make them less hazardous, to nonregulating countries where cheap and uninformed labor is abundant. \"Runaway shops\" then market their products in industrial nations with the competitive advantage of not having had to comply with costly workplace and pollution-control regulations. The international trade impacts of hazard export include: export of jobs from regulating to nonregulating countries; shift of international balance of payments in favor of nonregulating countries; export of mortal health hazards and environmental destruction to workers and communities in nonregulating nations, in order to produce goods for consumption by the regulating countries; weakened competitive position of reputable manufacturers who incur control costs and complete in domestic and would markets against less scrupulous companies; prolonged widespread use of discredited, extremely hazardous technologies, arising from the continuing \"subsidy\" of certain industries by workers and communities exposed to uncontrolled, well-recognized, mortal health hazards; and aggravated international relations resulting from developing nations' awareness and concern over becoming dumping grounds for hazard export from industrial nations.", "contents": "The export of hazardous factories to developing nations. The export of hazardous industrial plants to developing nations is examined for a number of industries. As hazardous and polluting industries come under increasing regulation in industrial nations, some of the affected processes are exported, without improvements to make them less hazardous, to nonregulating countries where cheap and uninformed labor is abundant. \"Runaway shops\" then market their products in industrial nations with the competitive advantage of not having had to comply with costly workplace and pollution-control regulations. The international trade impacts of hazard export include: export of jobs from regulating to nonregulating countries; shift of international balance of payments in favor of nonregulating countries; export of mortal health hazards and environmental destruction to workers and communities in nonregulating nations, in order to produce goods for consumption by the regulating countries; weakened competitive position of reputable manufacturers who incur control costs and complete in domestic and would markets against less scrupulous companies; prolonged widespread use of discredited, extremely hazardous technologies, arising from the continuing \"subsidy\" of certain industries by workers and communities exposed to uncontrolled, well-recognized, mortal health hazards; and aggravated international relations resulting from developing nations' awareness and concern over becoming dumping grounds for hazard export from industrial nations."}
{"id": "PMID:489184", "title": "Professionalism: rise and fall.", "content": "Historically, the early professionalization movements in medicine and the law appear as organizational projects which aspire to monopolize income and opportunities in markets of services or labor and to monopolize status and work privileges in occupational hierarchies. Their central task is to standardize training and link it to actual or potential markets of labor or services, a linkage that is structurally effected in the modern university. The second wave of professionalization has different protagonists than the older \"market professions\": placed in a different structural situation, the bureaucratic professions transform the model of profession (which they adopt as a strategy of collective ascension) into an ideology. The import of the ideology of professionalism is examined in relation to two issues: the relationships between professional occupations and bureaucratic organizations; and the position of professional occupations within the larger structure of inequality. Analysis of the first point requires consideration of the distinctions between professional occupations in the public and private sectors, the use of professional knowledge and the image of profession in bureaucratic organizations, and the specific characteristics of professions that produce their own knowledge. In the discussion of the second point, professional occupations and their ideology are examined in relation to other occupations and to the possibilities of political awareness generated by uncertain professional statuses.", "contents": "Professionalism: rise and fall. Historically, the early professionalization movements in medicine and the law appear as organizational projects which aspire to monopolize income and opportunities in markets of services or labor and to monopolize status and work privileges in occupational hierarchies. Their central task is to standardize training and link it to actual or potential markets of labor or services, a linkage that is structurally effected in the modern university. The second wave of professionalization has different protagonists than the older \"market professions\": placed in a different structural situation, the bureaucratic professions transform the model of profession (which they adopt as a strategy of collective ascension) into an ideology. The import of the ideology of professionalism is examined in relation to two issues: the relationships between professional occupations and bureaucratic organizations; and the position of professional occupations within the larger structure of inequality. Analysis of the first point requires consideration of the distinctions between professional occupations in the public and private sectors, the use of professional knowledge and the image of profession in bureaucratic organizations, and the specific characteristics of professions that produce their own knowledge. In the discussion of the second point, professional occupations and their ideology are examined in relation to other occupations and to the possibilities of political awareness generated by uncertain professional statuses."}
{"id": "PMID:489185", "title": "Monopolistic structures and industrial analysis in Spain: the case of the pharmaceutical industry.", "content": "This article seeks to illustrate the monopolistic structure of the Spanish pharmaceutical industry, focusing on its many dimensions. The basic conditions of technology and demand, product differentiation, effect of advertising, and barriers to entry are considered, as is financial and economic concentration. Although economic conditions are emphasized, the ways they affect public and private health, the quality of health services, and health education are also highlighted.", "contents": "Monopolistic structures and industrial analysis in Spain: the case of the pharmaceutical industry. This article seeks to illustrate the monopolistic structure of the Spanish pharmaceutical industry, focusing on its many dimensions. The basic conditions of technology and demand, product differentiation, effect of advertising, and barriers to entry are considered, as is financial and economic concentration. Although economic conditions are emphasized, the ways they affect public and private health, the quality of health services, and health education are also highlighted."}
{"id": "PMID:489192", "title": "Protective antiallergic effects of a new coumarin compound (BM 15.100) in experimental asthma.", "content": "The antiallergic properties of a new coumarin compound (BM 15.100) were tested in two separate trials, using bronchial provocation tests in asymptomatic volunteers with extrinsic allergic bronchial asthma. Study I was a blind study in 10 subjects with placebo controls and three additional non-medicated controls. Study II was an open study with non-medicated controls. Body plethysmography (study I) and spirometry (study II) were used for the assessment of bronchial obstruction. A significant protective effect of a single oral 20 mg dose of BM 15.100 administered 60 min prior to allergen inhalation was found in both trials. The effect of 10 mg BM 15.000 was not significant. Skin test, pulse rate, and diastolic blood pressure were not influenced. The results indicate a possible therapeutical value of BM 15.100.", "contents": "Protective antiallergic effects of a new coumarin compound (BM 15.100) in experimental asthma. The antiallergic properties of a new coumarin compound (BM 15.100) were tested in two separate trials, using bronchial provocation tests in asymptomatic volunteers with extrinsic allergic bronchial asthma. Study I was a blind study in 10 subjects with placebo controls and three additional non-medicated controls. Study II was an open study with non-medicated controls. Body plethysmography (study I) and spirometry (study II) were used for the assessment of bronchial obstruction. A significant protective effect of a single oral 20 mg dose of BM 15.100 administered 60 min prior to allergen inhalation was found in both trials. The effect of 10 mg BM 15.000 was not significant. Skin test, pulse rate, and diastolic blood pressure were not influenced. The results indicate a possible therapeutical value of BM 15.100."}
{"id": "PMID:489193", "title": "The cardiac electrophysiological effects of nifedipine.", "content": "A study was carried out on the electrophysiological effects of a sublingually administered antianginal drug: nifedipine (20 mg). The results show a significant shortening of sinus cycle length from 925 +/- 249 msec to 810 +/- 245 msec, (p less than 0.005) and the disappearance of some interpolation and echo zones. There are no significant effects on the other evaluated parameters of sino-atrial and AV-node function. In one case, during atrial pacing, a second-degree, Wenckebach type, A-V block was present only before nifedipine. The following conclusions were reached: 1. nifedipine has no significant electrophysiological effect on the human heart; 2. the electrophysiological effects observed are probably indirect and related to the vasodilating effect of the drug; 3. the absence of direct cardiac electrophysiological actions may be useful in patients suffering from coronary artery disease and presenting disturbances in the formation and/or conduction of the cardiac impulse.", "contents": "The cardiac electrophysiological effects of nifedipine. A study was carried out on the electrophysiological effects of a sublingually administered antianginal drug: nifedipine (20 mg). The results show a significant shortening of sinus cycle length from 925 +/- 249 msec to 810 +/- 245 msec, (p less than 0.005) and the disappearance of some interpolation and echo zones. There are no significant effects on the other evaluated parameters of sino-atrial and AV-node function. In one case, during atrial pacing, a second-degree, Wenckebach type, A-V block was present only before nifedipine. The following conclusions were reached: 1. nifedipine has no significant electrophysiological effect on the human heart; 2. the electrophysiological effects observed are probably indirect and related to the vasodilating effect of the drug; 3. the absence of direct cardiac electrophysiological actions may be useful in patients suffering from coronary artery disease and presenting disturbances in the formation and/or conduction of the cardiac impulse."}
{"id": "PMID:489194", "title": "Effect of spironolactone treatment on the renin-aldosterone system during pregnancy.", "content": "Ten pregnant patients were treated with spironolactone (100 mg daily) for two weeks. The patients were on a continuous long-term saluretic therapy for pregnancy edema. In addition, a potassium supplementation (30 mmol/day) was given until the beginning of spironolactone treatment. The patients thus treated had a mean urinary aldosterone excretion (dU-Aldo) of more than ten-fold in comparison to that of non pregnant state. Canrenone, the effective conversion product of spironolactone in plasma, reached its steady state level in three days as in non-pregnant subjects. dU-Aldo decreased in two weeks during the spironolactone by 36% (p less than 0.05). The inhibition of aldosterone secretion is thus evident also after a low clinical dose of spironolactone. Urinary potassium excretion (dU-K) decreased during the 1st day (p less than 0.05) and continued to decrease during one week of spironolactone therapy by a total of 21% (p less than 0.001). The decrease in dU-K reflects the cessation of potassium supplements in the beginning of the study. No changes in urinary sodium excretion were found. Plasma renin activity (PRA) increased in one week by 79 per cent and the changes of PRA and dU-Aldo showed inverse correlation (p less than 0.001). In our study natriuresis, cannot explain the increase of PRA. Our study suggests another feedback effect of aldosterone.", "contents": "Effect of spironolactone treatment on the renin-aldosterone system during pregnancy. Ten pregnant patients were treated with spironolactone (100 mg daily) for two weeks. The patients were on a continuous long-term saluretic therapy for pregnancy edema. In addition, a potassium supplementation (30 mmol/day) was given until the beginning of spironolactone treatment. The patients thus treated had a mean urinary aldosterone excretion (dU-Aldo) of more than ten-fold in comparison to that of non pregnant state. Canrenone, the effective conversion product of spironolactone in plasma, reached its steady state level in three days as in non-pregnant subjects. dU-Aldo decreased in two weeks during the spironolactone by 36% (p less than 0.05). The inhibition of aldosterone secretion is thus evident also after a low clinical dose of spironolactone. Urinary potassium excretion (dU-K) decreased during the 1st day (p less than 0.05) and continued to decrease during one week of spironolactone therapy by a total of 21% (p less than 0.001). The decrease in dU-K reflects the cessation of potassium supplements in the beginning of the study. No changes in urinary sodium excretion were found. Plasma renin activity (PRA) increased in one week by 79 per cent and the changes of PRA and dU-Aldo showed inverse correlation (p less than 0.001). In our study natriuresis, cannot explain the increase of PRA. Our study suggests another feedback effect of aldosterone."}
{"id": "PMID:489195", "title": "The effect of tricyclic antidepressants (TA) on the circulatory system in primary arterial hypertension.", "content": "The cardio-vascular reactions after i.m. application of 0.4 mg/kg of TA (imipramine, amitryptyline and nortryptyline) were compared in normotonics and patients suffering from essential hypertonia. It was found, that systolic blood pressure decreased significantly after the drugs only in hypertonic patients, whereas diastolic blood pressure fall was marked more in the normotonic group. In both groups, no apparent changes in heart action were noticed after the drugs. The TA caused a stronger increase of urine excretion of NE, E, VMA and NMN and MN in hypertonics than in normotonics.", "contents": "The effect of tricyclic antidepressants (TA) on the circulatory system in primary arterial hypertension. The cardio-vascular reactions after i.m. application of 0.4 mg/kg of TA (imipramine, amitryptyline and nortryptyline) were compared in normotonics and patients suffering from essential hypertonia. It was found, that systolic blood pressure decreased significantly after the drugs only in hypertonic patients, whereas diastolic blood pressure fall was marked more in the normotonic group. In both groups, no apparent changes in heart action were noticed after the drugs. The TA caused a stronger increase of urine excretion of NE, E, VMA and NMN and MN in hypertonics than in normotonics."}
{"id": "PMID:489196", "title": "Studies on kinetics of anturan excretion in man.", "content": "The kinetics of anturan excretion was studied. Experiments were carried out on ten healthy volunteers. The drug was given orally once applying three different doses: 100, 200, 400 mg. The contents of the drug in urine were determined by means of the modified method worked out by Wallace. It was found about 42 per cent of the dose was excreted with urine in an unchanged form. The process of anturan excretion may be described according to the one-compartment open kinetic model. The half-life of excretion is 3.5 hours, the excretion constant is 0.20. The formula showing the course of anturan excretion in time has been given. Five examples of the quantitative exposure test have been proposed; they allow the calculation of the absorbed drug dose and thus the degree of poisoning. The test can be also helpful in controlled therapy.", "contents": "Studies on kinetics of anturan excretion in man. The kinetics of anturan excretion was studied. Experiments were carried out on ten healthy volunteers. The drug was given orally once applying three different doses: 100, 200, 400 mg. The contents of the drug in urine were determined by means of the modified method worked out by Wallace. It was found about 42 per cent of the dose was excreted with urine in an unchanged form. The process of anturan excretion may be described according to the one-compartment open kinetic model. The half-life of excretion is 3.5 hours, the excretion constant is 0.20. The formula showing the course of anturan excretion in time has been given. Five examples of the quantitative exposure test have been proposed; they allow the calculation of the absorbed drug dose and thus the degree of poisoning. The test can be also helpful in controlled therapy."}
{"id": "PMID:489197", "title": "Effect of DDAVP on plasma renin activity in man.", "content": "We have investigated in eight healthy persons the effect of a single intranasal dose of 40 microgram DDAVP on plasma renin activity (PRA) in the upright posture. During normal sodium intake there were no differences in the PRA values between the time-control and DDAVP studies. However, during sodium restriction in four subjects with higher initial PRA, DDAVP induced a 40% decrease, lasting no longer than 45 minutes. There was no change in PRA after DDAVP in the other four subjects with much lower initial levels. Although no major or consistent suppressive influence could be demonstrated in the present study, in certain persons with highly stimulated initial PRA levels a transitory slight depression may be expected after a pharmacological dose of DDAVP.", "contents": "Effect of DDAVP on plasma renin activity in man. We have investigated in eight healthy persons the effect of a single intranasal dose of 40 microgram DDAVP on plasma renin activity (PRA) in the upright posture. During normal sodium intake there were no differences in the PRA values between the time-control and DDAVP studies. However, during sodium restriction in four subjects with higher initial PRA, DDAVP induced a 40% decrease, lasting no longer than 45 minutes. There was no change in PRA after DDAVP in the other four subjects with much lower initial levels. Although no major or consistent suppressive influence could be demonstrated in the present study, in certain persons with highly stimulated initial PRA levels a transitory slight depression may be expected after a pharmacological dose of DDAVP."}
{"id": "PMID:489198", "title": "Comparison of isoniazid phenotyping of black and white patients with emphasis on South African blacks.", "content": "Black tuberculosis patients from South Africa (S. A.) as well as from Birmingham, Alabama, U.S.A., showed a higher percentage of fast inactivators of isoniazid (INH) than that found in the North American white population, simultaneously sampled. In S. A. blacks, the frequency of fast inactivation was 57.9--59.6%, while in American blacks of Birmingham it amounted to 60.3%; in comparison to the above groups the rate of fast acetylators in Canadian Caucasians was 41.9% and in the USA white population 41.0%. For phenotyping of isoniazid inactivators a urine test was used. In this method the concentrations of INH (including isoniazidhydrazones) as well as acetylisoniazid were determined in the specimens collected 6--8 hrs following a test dose of 10 mg/kg INH.", "contents": "Comparison of isoniazid phenotyping of black and white patients with emphasis on South African blacks. Black tuberculosis patients from South Africa (S. A.) as well as from Birmingham, Alabama, U.S.A., showed a higher percentage of fast inactivators of isoniazid (INH) than that found in the North American white population, simultaneously sampled. In S. A. blacks, the frequency of fast inactivation was 57.9--59.6%, while in American blacks of Birmingham it amounted to 60.3%; in comparison to the above groups the rate of fast acetylators in Canadian Caucasians was 41.9% and in the USA white population 41.0%. For phenotyping of isoniazid inactivators a urine test was used. In this method the concentrations of INH (including isoniazidhydrazones) as well as acetylisoniazid were determined in the specimens collected 6--8 hrs following a test dose of 10 mg/kg INH."}
{"id": "PMID:489200", "title": "Systemic thermotherapy (whole body hyperthermia).", "content": "This is an article reviewing the literature and our experience to date (six months) in the treatment of cancer using whole body hyperthermia in the first 60 patients. WBHT is an effective method of treating cancer. Patients were treated for a total of eight hours, 180 degrees F for two hours. WBHT was induced by means of two high-flow water filled blankets. Toxicity included fatigue, nausea, diarrhea and first degree burns. There was no evidence of visceral damage. There were no mortalities during the procedure. Objective responses were 50%, subjective responses were 65%. The literature demonstrates and our study confirms that under closely monitored conditions, WBHT is a feasible, safe and active anti-cancer therapy. WBHT may be safely used as an adjunct to other active cancer therapies including X-ray therapy and chemotherapy.", "contents": "Systemic thermotherapy (whole body hyperthermia). This is an article reviewing the literature and our experience to date (six months) in the treatment of cancer using whole body hyperthermia in the first 60 patients. WBHT is an effective method of treating cancer. Patients were treated for a total of eight hours, 180 degrees F for two hours. WBHT was induced by means of two high-flow water filled blankets. Toxicity included fatigue, nausea, diarrhea and first degree burns. There was no evidence of visceral damage. There were no mortalities during the procedure. Objective responses were 50%, subjective responses were 65%. The literature demonstrates and our study confirms that under closely monitored conditions, WBHT is a feasible, safe and active anti-cancer therapy. WBHT may be safely used as an adjunct to other active cancer therapies including X-ray therapy and chemotherapy."}
{"id": "PMID:489201", "title": "Influence of metoprolol on hemodynamics and respiratory function in asthmatic patients.", "content": "Studies of metoprolol in asthmatic patients showed beta 1-selective blocking properties on the adrenergic receptor. Metoprolol in a dose of 40 mg given orally to 9 asthmatic patients significantly decreased the pulse rate at 60 and 120 minutes and the systolic blood pressure at 120 minutes but did not cause any increase of respiratory impedance, measured by the forced oscillation technique. A double-blind test was carried out to compare the effects of metoprolol and inactive placebo on the respiratory response to isoproterenol in 24 asthmatic patients. In the metoprolol group, systolic blood pressure decreased significantly and pulse rate at rest also tended to decrease. There was no significant difference between the metoprolol and placebo groups in the respiratory response to an isoproterenol aerosol.", "contents": "Influence of metoprolol on hemodynamics and respiratory function in asthmatic patients. Studies of metoprolol in asthmatic patients showed beta 1-selective blocking properties on the adrenergic receptor. Metoprolol in a dose of 40 mg given orally to 9 asthmatic patients significantly decreased the pulse rate at 60 and 120 minutes and the systolic blood pressure at 120 minutes but did not cause any increase of respiratory impedance, measured by the forced oscillation technique. A double-blind test was carried out to compare the effects of metoprolol and inactive placebo on the respiratory response to isoproterenol in 24 asthmatic patients. In the metoprolol group, systolic blood pressure decreased significantly and pulse rate at rest also tended to decrease. There was no significant difference between the metoprolol and placebo groups in the respiratory response to an isoproterenol aerosol."}
{"id": "PMID:489202", "title": "Drug interactions and side effects of hypolipidemic drugs.", "content": "The side effects and drug interactions of clofibrate, cholestyramine and nicotinic acid are reviewed because these hypolipidemic drugs are given for preventive and only rarely curative reasons and because these drugs have been given for many years and often concomitantly with other drugs.", "contents": "Drug interactions and side effects of hypolipidemic drugs. The side effects and drug interactions of clofibrate, cholestyramine and nicotinic acid are reviewed because these hypolipidemic drugs are given for preventive and only rarely curative reasons and because these drugs have been given for many years and often concomitantly with other drugs."}
{"id": "PMID:489203", "title": "Pharmacokinetics of amikacin (BB-K8) in patients undergoing hemodialysis.", "content": "The pharmacokinetics of Amikacin (BB-K8) was studied after administration in an i.v. bolus injection of 7.5 mg/kg to 10 patients with terminal renal impairment undergoing dialysis sessions of 6 hours. A two-compartment kinetic model has been used to describe the bi-phasic decrease of the plasma concentrations of the antibiotic thus establishing the amounts of the antibiotic in the central and peripheral compartments, its elimination being principally through the kidney. During the hemodialysis sessions the average pharmacokinetic parameters of the Amikacin were: alpha = 3.422 h-1 beta = 0.176 h-1 K12 = 1.820 h-1 K21 = 1.327 H-1, K13 = 0.450 h-1, Vc = 9.242 l Vp = 11.455 l Vdss = 20.697 l and delta = 0.377 l/kg. A dosage regimen as a function of the pharmacokinetic parameters is established for patients with terminal renal impairment which guarantees safe and efficient concentrations of the antibiotic.", "contents": "Pharmacokinetics of amikacin (BB-K8) in patients undergoing hemodialysis. The pharmacokinetics of Amikacin (BB-K8) was studied after administration in an i.v. bolus injection of 7.5 mg/kg to 10 patients with terminal renal impairment undergoing dialysis sessions of 6 hours. A two-compartment kinetic model has been used to describe the bi-phasic decrease of the plasma concentrations of the antibiotic thus establishing the amounts of the antibiotic in the central and peripheral compartments, its elimination being principally through the kidney. During the hemodialysis sessions the average pharmacokinetic parameters of the Amikacin were: alpha = 3.422 h-1 beta = 0.176 h-1 K12 = 1.820 h-1 K21 = 1.327 H-1, K13 = 0.450 h-1, Vc = 9.242 l Vp = 11.455 l Vdss = 20.697 l and delta = 0.377 l/kg. A dosage regimen as a function of the pharmacokinetic parameters is established for patients with terminal renal impairment which guarantees safe and efficient concentrations of the antibiotic."}
{"id": "PMID:489204", "title": "Clinical pharmacology studies with acenocoumarol.", "content": "In two different studies, the relation between plasma concentrations of acenocoumarol and apparent prothrombin levels (Quick test) was investigated, using a new specific analytical method for the assay of the drug. The prothrombin level appeared independent of the plasma concentration of the drug, which is higher in old patients (over 70 years) than in younger patients (under 51 years).", "contents": "Clinical pharmacology studies with acenocoumarol. In two different studies, the relation between plasma concentrations of acenocoumarol and apparent prothrombin levels (Quick test) was investigated, using a new specific analytical method for the assay of the drug. The prothrombin level appeared independent of the plasma concentration of the drug, which is higher in old patients (over 70 years) than in younger patients (under 51 years)."}
{"id": "PMID:489205", "title": "Pharmacokinetics of cefoxitin in patients undergoing hemodialysis.", "content": "The parmacokinetics of Cefoxitin were studied after an i.v. administration of 15 mg/kg body weight in 17 patients with terminal renal impairment, 10 of which were undergoing 6 hr hemodialysis sessions. The average pharmokinetic parameters obtained from this kind of patient were the following: alpha = 2.88 hr-1 beta = 0.18 hr-1 K12 = 1.43 hr-1 K21 = 1.04 hr-1 K13 = 0.53 hr-1 Vc = 8.23 l Vp = 11.61 l Vdss = 19.84 l. The amounts of the antibiotic in the central and peripheric compartments are established together with the amount of the antibiotic eliminated as a function of time. The pharmacokinetic parameters are significantly different from those established in the period between dialysis sessions, and thus, the elimination constant reaches a value of 0.28 h-1. The degree of plasma protein binding of Cefoxitin is 41.46% during the hemodialysis sessions. A dosage regimen is programmed as a function of the pharmacokinetic parameters established for this kind of patient. It is recommended that an i.v. dose of 15 mg/kg body weight should be administered at the beginning and end of each dialysis session lasting 6 hours, when the periods between the sessions are 48 hours.", "contents": "Pharmacokinetics of cefoxitin in patients undergoing hemodialysis. The parmacokinetics of Cefoxitin were studied after an i.v. administration of 15 mg/kg body weight in 17 patients with terminal renal impairment, 10 of which were undergoing 6 hr hemodialysis sessions. The average pharmokinetic parameters obtained from this kind of patient were the following: alpha = 2.88 hr-1 beta = 0.18 hr-1 K12 = 1.43 hr-1 K21 = 1.04 hr-1 K13 = 0.53 hr-1 Vc = 8.23 l Vp = 11.61 l Vdss = 19.84 l. The amounts of the antibiotic in the central and peripheric compartments are established together with the amount of the antibiotic eliminated as a function of time. The pharmacokinetic parameters are significantly different from those established in the period between dialysis sessions, and thus, the elimination constant reaches a value of 0.28 h-1. The degree of plasma protein binding of Cefoxitin is 41.46% during the hemodialysis sessions. A dosage regimen is programmed as a function of the pharmacokinetic parameters established for this kind of patient. It is recommended that an i.v. dose of 15 mg/kg body weight should be administered at the beginning and end of each dialysis session lasting 6 hours, when the periods between the sessions are 48 hours."}
{"id": "PMID:489206", "title": "Phenacetin concentrations in portal and hepatic venous blood in man.", "content": "After intra-gastric or intra-duodenal administration of phenacetin (200 mg in solution), concentrations of phenacetin and its 0-dealkylated metabolite, acetaminophen, in portal and hepatic veins and in peripheral blood were monitored. A sharp increase in the portal concentration of phenacetin indicated rapid absorption. The concentration ratio of acetaminophen to phenacetin in early portal blood was 0.01--0.11 and lower than the ratio in hepatic vein. The hepatic extraction ratio of phenacetin was calculated to be 0.59--0.78. It is concluded that 0-dealkylation occurs mainly in the liver and only to a limited extent in the gastrointestinal wall.", "contents": "Phenacetin concentrations in portal and hepatic venous blood in man. After intra-gastric or intra-duodenal administration of phenacetin (200 mg in solution), concentrations of phenacetin and its 0-dealkylated metabolite, acetaminophen, in portal and hepatic veins and in peripheral blood were monitored. A sharp increase in the portal concentration of phenacetin indicated rapid absorption. The concentration ratio of acetaminophen to phenacetin in early portal blood was 0.01--0.11 and lower than the ratio in hepatic vein. The hepatic extraction ratio of phenacetin was calculated to be 0.59--0.78. It is concluded that 0-dealkylation occurs mainly in the liver and only to a limited extent in the gastrointestinal wall."}
{"id": "PMID:489207", "title": "Dermatomyositis.", "content": "Dermatomyositis and polymyositis are diseases which must be carefully defined in each patient. Criteria such as those suggested by Bohan and Peter will help define the disease and exclude other possible causes of myositis. Immunological factors are probably involved in the pathogenesis of the disease, although varied types of injury may manifest  in clinically similar disease. Dermatomyositis, not polymyositis, is related to internal malignancy in adults; however, an extensive malignancy evaluation is not indicated. Therapy with corticosteroids will improve the quality of life, will prevent contractures and calcinosis and may improve the chance of survival. In cases where steroids are ineffective, immunosuppressives may be helpful.", "contents": "Dermatomyositis. Dermatomyositis and polymyositis are diseases which must be carefully defined in each patient. Criteria such as those suggested by Bohan and Peter will help define the disease and exclude other possible causes of myositis. Immunological factors are probably involved in the pathogenesis of the disease, although varied types of injury may manifest  in clinically similar disease. Dermatomyositis, not polymyositis, is related to internal malignancy in adults; however, an extensive malignancy evaluation is not indicated. Therapy with corticosteroids will improve the quality of life, will prevent contractures and calcinosis and may improve the chance of survival. In cases where steroids are ineffective, immunosuppressives may be helpful."}
{"id": "PMID:489217", "title": "The determination of polycyclic aromatic hydrocarbons at the ng/L level in Ottawa tap water.", "content": "Samples of Ottawa drinking water, collected in January and February 1978 were analysed by Gas Chromatography/Mass Spectrometry (GC/MS) for fifty polycyclic aromatic hydrocarbons (PAH) and five oxygenated polycyclic aromatic hydrocarbons (O-PAH), following extraction of the organic species using Amberlite XAD-2 macroreticular resin. In the January sample, thirty PAHs, ranging in concentration from 0.05 to 14 ng/L with a mean value of 3.8 ng/L and a total weight of 114 ng/L and four O-PAHs, ranging in concentration from 0.10 to 1.8 ng/L with a mean value of 0.91 ng/L and a total weight of 3.7 ng/L, were detected. In the February sample, thirty-six PAHs, ranging in concentration from 0.05 to 8.1 ng/L with a mean value of 1.4 ng/L and a total weight of 50.4 ng/L and five O-PAHs, ranging in concentration from 0.20 to 2.4 ng/L with a mean value of 1.0 ng/L and a total weight of 5.2 ng/L, were detected. Twenty-eight PAHs and four O-pahs were common to the two samples. The use of Amberlite XAD-2 macroreticular resin to extract PAHs from drinking water is shown to be effective, although the recovery of individual PAHs from artificially loaded XAD-2 resin varied from 57 to 100%.", "contents": "The determination of polycyclic aromatic hydrocarbons at the ng/L level in Ottawa tap water. Samples of Ottawa drinking water, collected in January and February 1978 were analysed by Gas Chromatography/Mass Spectrometry (GC/MS) for fifty polycyclic aromatic hydrocarbons (PAH) and five oxygenated polycyclic aromatic hydrocarbons (O-PAH), following extraction of the organic species using Amberlite XAD-2 macroreticular resin. In the January sample, thirty PAHs, ranging in concentration from 0.05 to 14 ng/L with a mean value of 3.8 ng/L and a total weight of 114 ng/L and four O-PAHs, ranging in concentration from 0.10 to 1.8 ng/L with a mean value of 0.91 ng/L and a total weight of 3.7 ng/L, were detected. In the February sample, thirty-six PAHs, ranging in concentration from 0.05 to 8.1 ng/L with a mean value of 1.4 ng/L and a total weight of 50.4 ng/L and five O-PAHs, ranging in concentration from 0.20 to 2.4 ng/L with a mean value of 1.0 ng/L and a total weight of 5.2 ng/L, were detected. Twenty-eight PAHs and four O-pahs were common to the two samples. The use of Amberlite XAD-2 macroreticular resin to extract PAHs from drinking water is shown to be effective, although the recovery of individual PAHs from artificially loaded XAD-2 resin varied from 57 to 100%."}
{"id": "PMID:489218", "title": "Colored gift wrapping papers as a potential source of toxic metals.", "content": "Sanitary land fills are the alternate to waste incineration in New Jersey. While industrial waste disposal is controlled, few restrictions apply to the disposal of domestic solid waste. Among the materials of concern from domestic sources are colored gift wrapping papers. Cadmium, chromium, copper, iron, manganese, molybdenum, nickel, lead and zinc were determined in some dozen and a half samples of gift wrapping paper by atomic absorption spectrometry after wet ashing and after simulated leaching. High levels of lead and chromium were found in many of the papers. The leachates showed correspondingly high levels of lead.", "contents": "Colored gift wrapping papers as a potential source of toxic metals. Sanitary land fills are the alternate to waste incineration in New Jersey. While industrial waste disposal is controlled, few restrictions apply to the disposal of domestic solid waste. Among the materials of concern from domestic sources are colored gift wrapping papers. Cadmium, chromium, copper, iron, manganese, molybdenum, nickel, lead and zinc were determined in some dozen and a half samples of gift wrapping paper by atomic absorption spectrometry after wet ashing and after simulated leaching. High levels of lead and chromium were found in many of the papers. The leachates showed correspondingly high levels of lead."}
{"id": "PMID:489219", "title": "Rapid separation on copper powder of total mercury in blood and determination of mercury by flameless atomic absorption spectrometry.", "content": "The determination of mercury in blood by flameless atomic absorption spectrometry (FAAS) has been described. Prior to its analysis, the sample was decomposed by combustion and separated on a copper powder micro-column. A special type of cell has been used which gives a better sensitivity compared with the types of cells described in the literature and the method of FAAS analysis has been improved. The sensitivity of 0.1 ng for 1% absorbance was observed and the standard deviation for six determinations at this level was found to be +/- 0.05 ng, for 95% probability.", "contents": "Rapid separation on copper powder of total mercury in blood and determination of mercury by flameless atomic absorption spectrometry. The determination of mercury in blood by flameless atomic absorption spectrometry (FAAS) has been described. Prior to its analysis, the sample was decomposed by combustion and separated on a copper powder micro-column. A special type of cell has been used which gives a better sensitivity compared with the types of cells described in the literature and the method of FAAS analysis has been improved. The sensitivity of 0.1 ng for 1% absorbance was observed and the standard deviation for six determinations at this level was found to be +/- 0.05 ng, for 95% probability."}
{"id": "PMID:489220", "title": "The Chatham Blood Pressure Study. An application of Bayesian growth curve models to a longitudinal study of blood pressure in children.", "content": "Recent developments in statistics have produced powerful methods that facilitate the analysis of longitudinal studies. These methods are illustrated by an analysis of a longitudinal study of blood pressure in children. The results of the study show a clear tendency for blood pressure to increase with age, and Asian children tend to have lower blood pressures than their Caucasian counterparts of the same age. There is evidence to support the hypothesis that blood pressures track.", "contents": "The Chatham Blood Pressure Study. An application of Bayesian growth curve models to a longitudinal study of blood pressure in children. Recent developments in statistics have produced powerful methods that facilitate the analysis of longitudinal studies. These methods are illustrated by an analysis of a longitudinal study of blood pressure in children. The results of the study show a clear tendency for blood pressure to increase with age, and Asian children tend to have lower blood pressures than their Caucasian counterparts of the same age. There is evidence to support the hypothesis that blood pressures track."}
{"id": "PMID:489221", "title": "Regional differences in risk factor distributions, food habits and coronary heart disease mortality and morbidity in Belgium.", "content": "Belgium can be divided in 4 major geographical areas: two Dutch speaking areas in the north (Campine and Flanders), a French speaking area in the south (Wallonia) and the Brussels area in between. Significant differences in mean serum cholesterol levels were observed with the lowest level in Campine, intermediate in Flanders and Brussels, and the highest level in Wallonia. Similar differences were observed in the prevalence of CHD and mortality from CHD is also higher in the French speaking part of the country. A survey of food habits since 1959 shows a higher butter and lower soft margarine consumption in the south. However, differences in smoking habits and personality traits also exist.", "contents": "Regional differences in risk factor distributions, food habits and coronary heart disease mortality and morbidity in Belgium. Belgium can be divided in 4 major geographical areas: two Dutch speaking areas in the north (Campine and Flanders), a French speaking area in the south (Wallonia) and the Brussels area in between. Significant differences in mean serum cholesterol levels were observed with the lowest level in Campine, intermediate in Flanders and Brussels, and the highest level in Wallonia. Similar differences were observed in the prevalence of CHD and mortality from CHD is also higher in the French speaking part of the country. A survey of food habits since 1959 shows a higher butter and lower soft margarine consumption in the south. However, differences in smoking habits and personality traits also exist."}
{"id": "PMID:489222", "title": "Breast cancer incidence: geographical correlations in Finland.", "content": "Correlation analysis was performed on breast cancer incidence and risk factors in geographical areas of Finland. Breast cancer risk is associated with fertility and taxable income but not with the size of the woman. Trends in risk factors indicate that the rapid increase in the incidence of breast cancer is likely to continue. It is concluded that factors which are reflected by the standard of living and fertility might act independently and not through the nutritional status, for which the size of the woman is an operational indicator.", "contents": "Breast cancer incidence: geographical correlations in Finland. Correlation analysis was performed on breast cancer incidence and risk factors in geographical areas of Finland. Breast cancer risk is associated with fertility and taxable income but not with the size of the woman. Trends in risk factors indicate that the rapid increase in the incidence of breast cancer is likely to continue. It is concluded that factors which are reflected by the standard of living and fertility might act independently and not through the nutritional status, for which the size of the woman is an operational indicator."}
{"id": "PMID:489223", "title": "Cohort analysis of lung cancer in the Netherlands.", "content": "A cohort analysis of lung cancer mortality in the Netherlands from 1936--1974 is presented. Apart from small corrections, age-specific lung cancer mortality rates can be expressed as the product of a cohort (generation) factor and an age factor. The cohort factors increase until the 1930 birth cohort, after which there is a rapid decline. The age-risk factors increase smoothy with age. Age-specific incidence curves of past and future years will follow the curve of the age-risk factors provided that the cohort-risk factors are unchanging. The method can be applied to other chronic diseases.", "contents": "Cohort analysis of lung cancer in the Netherlands. A cohort analysis of lung cancer mortality in the Netherlands from 1936--1974 is presented. Apart from small corrections, age-specific lung cancer mortality rates can be expressed as the product of a cohort (generation) factor and an age factor. The cohort factors increase until the 1930 birth cohort, after which there is a rapid decline. The age-risk factors increase smoothy with age. Age-specific incidence curves of past and future years will follow the curve of the age-risk factors provided that the cohort-risk factors are unchanging. The method can be applied to other chronic diseases."}
{"id": "PMID:489224", "title": "Non-polio enterovirus disease in the United States, 1971--1975.", "content": "Reports to the Center for Disease Control on isolation of non-polio enteroviruses for the years 1971--1975 were analysed. During the 5 year period, enterovirus isolations were reported from 7 075 individuals. 90% of these occurred in the 7 month interval of May--November. Enteroviruses were isolated more frequently from males than females for all age groups in all 5 years. The incidence of reported isolations decreased with increasing age, and an inverse relationship between severity of disease and age was suggested. Clinical diagnoses associated with enteroviral isolations included aseptic meningitis, encephalitis, upper respiratory tract disease, non-specific febrile illness, gastroenteritis, pneumonia and lower respiratory tract disease, exanthem, and enanthem.", "contents": "Non-polio enterovirus disease in the United States, 1971--1975. Reports to the Center for Disease Control on isolation of non-polio enteroviruses for the years 1971--1975 were analysed. During the 5 year period, enterovirus isolations were reported from 7 075 individuals. 90% of these occurred in the 7 month interval of May--November. Enteroviruses were isolated more frequently from males than females for all age groups in all 5 years. The incidence of reported isolations decreased with increasing age, and an inverse relationship between severity of disease and age was suggested. Clinical diagnoses associated with enteroviral isolations included aseptic meningitis, encephalitis, upper respiratory tract disease, non-specific febrile illness, gastroenteritis, pneumonia and lower respiratory tract disease, exanthem, and enanthem."}
{"id": "PMID:489225", "title": "Blood pressure and migration in children.", "content": "The effect of migration on childhood blood pressure levels has been studied by comparing children before and after migration to New Zealand with children who stayed at home on the Pacific atolls of Tokelau. Data were collected in 1971 on 502 children (97% response rate) aged 5--14 years resident in Tokelau and follow-up data were collected in New Zealand and in Tokelau in 1975--1977 (respknse rate 91%). No selection factors were detected before migration. After migration, the younger migrants had significantly higher blood pressures and were heavier, but not taller, than the non-migrants. Weight differences explained some but not all of the blood pressure differences. There were no differences in body size between the 2 groups of older children although the older non migrant girls had higher blood pressure than the migrant girls.", "contents": "Blood pressure and migration in children. The effect of migration on childhood blood pressure levels has been studied by comparing children before and after migration to New Zealand with children who stayed at home on the Pacific atolls of Tokelau. Data were collected in 1971 on 502 children (97% response rate) aged 5--14 years resident in Tokelau and follow-up data were collected in New Zealand and in Tokelau in 1975--1977 (respknse rate 91%). No selection factors were detected before migration. After migration, the younger migrants had significantly higher blood pressures and were heavier, but not taller, than the non-migrants. Weight differences explained some but not all of the blood pressure differences. There were no differences in body size between the 2 groups of older children although the older non migrant girls had higher blood pressure than the migrant girls."}
{"id": "PMID:489227", "title": "The prevalence of diabetes mellitus in a rural community in Iraq.", "content": "A survey was undertaken to estimate the prevalence of diabetes mellitus in a rural community in Iraq. Blood-sugar estimations after glucose-loading were performed on a sample of a village population 15 + years. 4.8% had diabetes and a majority of them had symptoms referable to their diabetic state. Diabetes was totally unknown among this rural population before the survey.", "contents": "The prevalence of diabetes mellitus in a rural community in Iraq. A survey was undertaken to estimate the prevalence of diabetes mellitus in a rural community in Iraq. Blood-sugar estimations after glucose-loading were performed on a sample of a village population 15 + years. 4.8% had diabetes and a majority of them had symptoms referable to their diabetic state. Diabetes was totally unknown among this rural population before the survey."}
{"id": "PMID:489228", "title": "Statistical problems in studying the relative specificities of association between environmental agents and different diseases: a solution suggested.", "content": "If an environmental agent is strongly associated with only one disease then that is greater evidence in favour of a casual relationship than if that agent were also strongly associated with other unrelated diseases. Standard regression and correlation analysis are shown to be incapable of answering questions about disease specificity. A simple index, based upon regression, is proposed. The standardised regression coefficients in a regression equation based on the logistic transformation of a death rate are shown to have a particularly simple interpretation in terms of our index. The work is illustrated on an example drawn from the controversy concerning the association between area differences in ischaemic heart disease mortality and water hardness.", "contents": "Statistical problems in studying the relative specificities of association between environmental agents and different diseases: a solution suggested. If an environmental agent is strongly associated with only one disease then that is greater evidence in favour of a casual relationship than if that agent were also strongly associated with other unrelated diseases. Standard regression and correlation analysis are shown to be incapable of answering questions about disease specificity. A simple index, based upon regression, is proposed. The standardised regression coefficients in a regression equation based on the logistic transformation of a death rate are shown to have a particularly simple interpretation in terms of our index. The work is illustrated on an example drawn from the controversy concerning the association between area differences in ischaemic heart disease mortality and water hardness."}
{"id": "PMID:489229", "title": "A note on Wong's competing risk model.", "content": "It is suggested that a method proposed recently by Wong contains an unrealistic assumption. This can be avoided by making an alternative assumption which leads to a slight alteration to one of his equations.", "contents": "A note on Wong's competing risk model. It is suggested that a method proposed recently by Wong contains an unrealistic assumption. This can be avoided by making an alternative assumption which leads to a slight alteration to one of his equations."}
{"id": "PMID:489243", "title": "Thermal activation of peroxidase from tobacco leaf mesophyll cell walls.", "content": "Cell wall-bound peroxidase isolated from tobacco leaf mesophyll cell walls was found to consist of two isoenzymes (P1 and P2). Each exists in the form of a single subunit with the same molecular weight (35 000) as determined by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Both isoenzymes exhibited maximum activities at 70 degrees. P1 increased to 265% and P2 to 140% of the activities assayed at 27 degrees; below this temperature the two isoenzymes had the same specific activity. On hydrolysis, P1 showed a carbohydrate content of 26.22% when the monosaccharides were analyzed by gas liquid chromatography; P2 gave 21.45%.", "contents": "Thermal activation of peroxidase from tobacco leaf mesophyll cell walls. Cell wall-bound peroxidase isolated from tobacco leaf mesophyll cell walls was found to consist of two isoenzymes (P1 and P2). Each exists in the form of a single subunit with the same molecular weight (35 000) as determined by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Both isoenzymes exhibited maximum activities at 70 degrees. P1 increased to 265% and P2 to 140% of the activities assayed at 27 degrees; below this temperature the two isoenzymes had the same specific activity. On hydrolysis, P1 showed a carbohydrate content of 26.22% when the monosaccharides were analyzed by gas liquid chromatography; P2 gave 21.45%."}
{"id": "PMID:489244", "title": "Peptide--water association in peptide crystals.", "content": "The structures of 37 peptide crystals, containing 78 water-peptide hydrogen bonds and 77 other hydrogen bonds involving water, were surveyed to identify the geometry of peptide backbone hydration. In the sample, hydration of peptide carbonyl occurred more frequently than hydration of peptide N--H. The most probable value of the C'=O ... O water angle was near 138 degrees, considerably greater than the 120 degrees to the axis of a lone electron pair on the carbonyl oxygen. Associated water oxygens tended to be in the plane of the peptide bond, bui--H and Ci+1=O atoms, was common in glycine-containing cyclic hexapeptides. The distribution of angles between two hydrogen bonds at a single water molecule, as defined by the three nonhydrogen atoms involved, was centered near the tetrahedral angle.", "contents": "Peptide--water association in peptide crystals. The structures of 37 peptide crystals, containing 78 water-peptide hydrogen bonds and 77 other hydrogen bonds involving water, were surveyed to identify the geometry of peptide backbone hydration. In the sample, hydration of peptide carbonyl occurred more frequently than hydration of peptide N--H. The most probable value of the C'=O ... O water angle was near 138 degrees, considerably greater than the 120 degrees to the axis of a lone electron pair on the carbonyl oxygen. Associated water oxygens tended to be in the plane of the peptide bond, bui--H and Ci+1=O atoms, was common in glycine-containing cyclic hexapeptides. The distribution of angles between two hydrogen bonds at a single water molecule, as defined by the three nonhydrogen atoms involved, was centered near the tetrahedral angle."}
{"id": "PMID:489245", "title": "Urea and guanidine hydrochloride induced dissociation and denaturation of bacteriophage F2 capsid.", "content": "A single, low molecular weight protein is found after urea or guanidine hydrochloride (Gdn.HCl) treatment of empty capsids derived from bacteriophage f2. The final product of denaturation is apparently a monomer, existing as a random coil in larger than or equal to 4.0 M Gdn.HCl but in a less extended form in 8.0 M urea. In contrast, an 11 S protein component is isolated after treatment of the intact virus with 4.0 M Gdn.HCl (Zelazo & Haschemeyer, 1969), indicating that RNA plays a role in stabilizing larger subunits. Denaturation by Gdn.HCl occurs in two stages as measured by changes in CD and Stokes radius: dissociation that involves a structural perturbation of aromatic side chains, followed by a major, cooperative transition that evidently results in the loss of all noncovalent structure. Denaturation by urea appears to be a much less cooperative process that occurs in several steps over a wide range of urea concentration (1--7 M). In both urea and Gdn.HCl, dissociation into subunits begins at a lower concentration of denaturant than the major changes in conformation.", "contents": "Urea and guanidine hydrochloride induced dissociation and denaturation of bacteriophage F2 capsid. A single, low molecular weight protein is found after urea or guanidine hydrochloride (Gdn.HCl) treatment of empty capsids derived from bacteriophage f2. The final product of denaturation is apparently a monomer, existing as a random coil in larger than or equal to 4.0 M Gdn.HCl but in a less extended form in 8.0 M urea. In contrast, an 11 S protein component is isolated after treatment of the intact virus with 4.0 M Gdn.HCl (Zelazo & Haschemeyer, 1969), indicating that RNA plays a role in stabilizing larger subunits. Denaturation by Gdn.HCl occurs in two stages as measured by changes in CD and Stokes radius: dissociation that involves a structural perturbation of aromatic side chains, followed by a major, cooperative transition that evidently results in the loss of all noncovalent structure. Denaturation by urea appears to be a much less cooperative process that occurs in several steps over a wide range of urea concentration (1--7 M). In both urea and Gdn.HCl, dissociation into subunits begins at a lower concentration of denaturant than the major changes in conformation."}
{"id": "PMID:489246", "title": "Synthesis of analogs of the serum thymic nonapeptide, \"facteur thymique serique\" (FTS). Part I.", "content": "Analogs of FTS (Facteur Thymique S\u00e9rique), less than :formula: (see text), a circulating thymic factor, were prepared by replacing the amino acid residues in positions 1, 2, 8 and 9, or by shortening the nonapeptide chain at the N- or C-terminal end. These peptides were synthesized by two different schemes using the conventional synthesis in solution.", "contents": "Synthesis of analogs of the serum thymic nonapeptide, \"facteur thymique serique\" (FTS). Part I. Analogs of FTS (Facteur Thymique S\u00e9rique), less than :formula: (see text), a circulating thymic factor, were prepared by replacing the amino acid residues in positions 1, 2, 8 and 9, or by shortening the nonapeptide chain at the N- or C-terminal end. These peptides were synthesized by two different schemes using the conventional synthesis in solution."}
{"id": "PMID:489247", "title": "Chemical, biological and immunological characterization of separated electrophoretic components of human chorionic somatomammotropin (HCS).", "content": "Purified HCS preparations ofter show heterogeneity when assayed by electrophoresis, although they may be considered homogeneous according to other criteria (N-terminal amino acid analysis, gel filtration and ultracentrifugation patterns, etc.). Using DEAE-cellulose chromatography, the two main components corresponding to various degrees of hormone amidation were separated. The deamidation kinetic of the electrophoretically slower migrating component was studied and found to be sensitive to temperature and, to a lesser extent, to pH. Denaturing agents strongly increased the deamidation rate, while no effect was found by raising the ionic strength. No difference was evident between the two components and the parent hormone when compared (on a weight basis) in a specific HCS radioimmunoassay system. Even the precipitin reaction in agar gel with an anti-human growth hormone (HGH) serum did not show variations in cross-reactivity versus HGH. Moreover, groups of guinea pigs immunized with the single HCS components, gave antibodies reacting identically with the components and the parent HCS. The prolactin-like activity of the two components differed when determined by the pigeon crop-sac asay: the slower migrating component proved to be less active than the more deamidated one. On the other hand, the activity in two different radioreceptor assays (employing membranes from rabbit mammary gland and rat ventral prostate) was greater for the faster migrating component.", "contents": "Chemical, biological and immunological characterization of separated electrophoretic components of human chorionic somatomammotropin (HCS). Purified HCS preparations ofter show heterogeneity when assayed by electrophoresis, although they may be considered homogeneous according to other criteria (N-terminal amino acid analysis, gel filtration and ultracentrifugation patterns, etc.). Using DEAE-cellulose chromatography, the two main components corresponding to various degrees of hormone amidation were separated. The deamidation kinetic of the electrophoretically slower migrating component was studied and found to be sensitive to temperature and, to a lesser extent, to pH. Denaturing agents strongly increased the deamidation rate, while no effect was found by raising the ionic strength. No difference was evident between the two components and the parent hormone when compared (on a weight basis) in a specific HCS radioimmunoassay system. Even the precipitin reaction in agar gel with an anti-human growth hormone (HGH) serum did not show variations in cross-reactivity versus HGH. Moreover, groups of guinea pigs immunized with the single HCS components, gave antibodies reacting identically with the components and the parent HCS. The prolactin-like activity of the two components differed when determined by the pigeon crop-sac asay: the slower migrating component proved to be less active than the more deamidated one. On the other hand, the activity in two different radioreceptor assays (employing membranes from rabbit mammary gland and rat ventral prostate) was greater for the faster migrating component."}
{"id": "PMID:489249", "title": "Synthesis, resolution and charge-donor properties of six tryptophan analogues.", "content": "In order to investigate the special function of tryptophan in peptide hormones, six tryptophan analogues have been synthesized, in which structural resemblance has been grossly retained and potentially essential properties have only partially been varied. The L-enantiomers have been isolated after enzymatic digestion of racemic dipeptide derivatives, and charge-transfer properties of the compounds have been studied.", "contents": "Synthesis, resolution and charge-donor properties of six tryptophan analogues. In order to investigate the special function of tryptophan in peptide hormones, six tryptophan analogues have been synthesized, in which structural resemblance has been grossly retained and potentially essential properties have only partially been varied. The L-enantiomers have been isolated after enzymatic digestion of racemic dipeptide derivatives, and charge-transfer properties of the compounds have been studied."}
{"id": "PMID:489250", "title": "Trinitrophenylation of bovine growth hormone.", "content": "Bovine growth hormone was chemically modified with picryl sulfonic acid, at pH 8.4 during 2 and 5 min of reaction. The N-terminal residue provides the most reactive amino group followed by the epsilon-amino groups of lysine 179 and lysines 143, 69, 111, 170 and 166 in decreasing order. These results agree with those obtained previously with equine growth hormone, except that residue 156 is not modified in bovine growth hormone. An important decrease in biological activity occurs between 2 and 5 min of reaction without sensible modification in the alpha-helix content of the molecule.", "contents": "Trinitrophenylation of bovine growth hormone. Bovine growth hormone was chemically modified with picryl sulfonic acid, at pH 8.4 during 2 and 5 min of reaction. The N-terminal residue provides the most reactive amino group followed by the epsilon-amino groups of lysine 179 and lysines 143, 69, 111, 170 and 166 in decreasing order. These results agree with those obtained previously with equine growth hormone, except that residue 156 is not modified in bovine growth hormone. An important decrease in biological activity occurs between 2 and 5 min of reaction without sensible modification in the alpha-helix content of the molecule."}
{"id": "PMID:489251", "title": "N.M.R. of 'oriented' monosubstituted amides--cis and trans N-methyl formamide.", "content": "The n.m.r. spectrum of N-methyl formamide oriented in a lyotropic liquid crystal solvent shows the presence of the cis as well as the trans forms. Analysis of the spectra due to both the species has been carried out. Possible non-planar distortions at the nitrogen atom for the trans species have been computed using the derived dipolar couplings and the available structural data.", "contents": "N.M.R. of 'oriented' monosubstituted amides--cis and trans N-methyl formamide. The n.m.r. spectrum of N-methyl formamide oriented in a lyotropic liquid crystal solvent shows the presence of the cis as well as the trans forms. Analysis of the spectra due to both the species has been carried out. Possible non-planar distortions at the nitrogen atom for the trans species have been computed using the derived dipolar couplings and the available structural data."}
{"id": "PMID:489252", "title": "Conformational analysis of N-(tert.-amyloxycarbony-L-proline in the solid state and in solution.", "content": "The solid-state conformational analysis of t-AOC-L-Pro-OH has indicated that the molecules are not folded up to form an oxy-C7 peptide conformation, but rather that they are held together through intermolecular O-H .... 0 = C (urethane) hydrogen bonds. The tertiary amide bond is in the cis configuration. In solvents of high polarity strongly solvated species largely predominate. In cyclohexane solution non-associated and associated (involving the carboxyl C = O as the proton acceptor) species are simultaneously present. Obviously, the extent of association increases with increasing solute concentration. The amount of the oxy-C7 form, if any, should be extremely small. It is also demonstrated that CD measurements alone can lead to an incorrect picture of the conformational preferences of amino acid derivatives and small peptides in solution.", "contents": "Conformational analysis of N-(tert.-amyloxycarbony-L-proline in the solid state and in solution. The solid-state conformational analysis of t-AOC-L-Pro-OH has indicated that the molecules are not folded up to form an oxy-C7 peptide conformation, but rather that they are held together through intermolecular O-H .... 0 = C (urethane) hydrogen bonds. The tertiary amide bond is in the cis configuration. In solvents of high polarity strongly solvated species largely predominate. In cyclohexane solution non-associated and associated (involving the carboxyl C = O as the proton acceptor) species are simultaneously present. Obviously, the extent of association increases with increasing solute concentration. The amount of the oxy-C7 form, if any, should be extremely small. It is also demonstrated that CD measurements alone can lead to an incorrect picture of the conformational preferences of amino acid derivatives and small peptides in solution."}
{"id": "PMID:489253", "title": "Protamines. III. Synthesis of the tetradecapeptide corresponding to the C-terminal sequence 52--65 of galline.", "content": "The synthesis of peptides containing blocks of arginyl residues is proposed through amidination of the corresponding ornithyl analogs. In order to test this strategy the ornithyl analog of the C-terminal sequence 52--65 of galline was synthesized by the conventional method. The amidination reaction, performed on fragments of different length and ornithyl-residue content, quantitatively converts ornithines into arginines. The strategy proposed may represent a powerful tool for the synthesis of protamines and other basic proteins.", "contents": "Protamines. III. Synthesis of the tetradecapeptide corresponding to the C-terminal sequence 52--65 of galline. The synthesis of peptides containing blocks of arginyl residues is proposed through amidination of the corresponding ornithyl analogs. In order to test this strategy the ornithyl analog of the C-terminal sequence 52--65 of galline was synthesized by the conventional method. The amidination reaction, performed on fragments of different length and ornithyl-residue content, quantitatively converts ornithines into arginines. The strategy proposed may represent a powerful tool for the synthesis of protamines and other basic proteins."}
{"id": "PMID:489254", "title": "Electron microscope study on human ceruloplasmin.", "content": "Electron microscopy of human ceruloplasmin (CP) molecules revealed a few distinctive types of particle images. Analysis of these images allows to propose a tentative model for CP: six \"subunits\" (which we call domains) not much different in size are arranged with 32 point group pseudosymmetry. The determination of the number of polypeptides arising at the spontaneous specific proteolytic fragmentation of CP and their molecular weights conform with this assumption. The electrophoretic studies of the CP samples prepared both with and without potent proteolytic inhibitor, PMSF, revealed that CP is a single-chain protein with molecular weight of 130 000. Isolated and stored without PMSF the polypeptide chain of CP undergoes specific proteolytic cleavage which results in the appearance of polypeptides with molecular weights of 16 000, 48 000, and 64 000. The latter two polypeptides degradate to about two- and three-fold decreased molecular weights fragments, respectively. Therefore, the single polypeptide chain of CP contains at least five peptide bonds which are particularly susceptible to proteolytic attack and which connect six principal segments of the chain. The hydrolysis of these bonds results in liberation of the six fragments which were integrated in the enzymatically active globule of CP.", "contents": "Electron microscope study on human ceruloplasmin. Electron microscopy of human ceruloplasmin (CP) molecules revealed a few distinctive types of particle images. Analysis of these images allows to propose a tentative model for CP: six \"subunits\" (which we call domains) not much different in size are arranged with 32 point group pseudosymmetry. The determination of the number of polypeptides arising at the spontaneous specific proteolytic fragmentation of CP and their molecular weights conform with this assumption. The electrophoretic studies of the CP samples prepared both with and without potent proteolytic inhibitor, PMSF, revealed that CP is a single-chain protein with molecular weight of 130 000. Isolated and stored without PMSF the polypeptide chain of CP undergoes specific proteolytic cleavage which results in the appearance of polypeptides with molecular weights of 16 000, 48 000, and 64 000. The latter two polypeptides degradate to about two- and three-fold decreased molecular weights fragments, respectively. Therefore, the single polypeptide chain of CP contains at least five peptide bonds which are particularly susceptible to proteolytic attack and which connect six principal segments of the chain. The hydrolysis of these bonds results in liberation of the six fragments which were integrated in the enzymatically active globule of CP."}
{"id": "PMID:489255", "title": "Studies on peptides. LXXXI. Application of a new arginine derivative, NG-mesitylene-2-sulfonylarginine, to the synthesis of substance P and neurotensin.", "content": "A new devised arginine derivative, NG-mesitylene-2-sulfonylarginine, Arg(Mts), was employed for the synthesis of hypothalamic substance P and neurotensin. The former was obtained in 74% yield by treatment of the protected undecapeptide amide, Z - Arg(Mts) - Pro - Lys(Z) - Pro - Gln - Gln - Phe - Phe - Gly - Leu - Met(O)-NH2, with methanesulfonic acid in the presence of anisole followed by reduction of the sulfoxide with 2-mercaptoethanol. The latter was obtained in 54% yield by the similar treatment of the protected tridecapeptide ester, Z - Pyr - Leu - Tyr - Glu(OBzl) - Asn - Lys(Z) - Pro - Arg(Mts) - Arg(Mts) - Pro - Tyr - Ile - Leu - OBzl, with methanesulfonic acid. As scavenger, a mixture of anisole-thioanisole-o-cresol (1:1:1, by vol.) was employed to suppress the side reaction, O-mesitylene-2-sulfonation of the Tyr residue.", "contents": "Studies on peptides. LXXXI. Application of a new arginine derivative, NG-mesitylene-2-sulfonylarginine, to the synthesis of substance P and neurotensin. A new devised arginine derivative, NG-mesitylene-2-sulfonylarginine, Arg(Mts), was employed for the synthesis of hypothalamic substance P and neurotensin. The former was obtained in 74% yield by treatment of the protected undecapeptide amide, Z - Arg(Mts) - Pro - Lys(Z) - Pro - Gln - Gln - Phe - Phe - Gly - Leu - Met(O)-NH2, with methanesulfonic acid in the presence of anisole followed by reduction of the sulfoxide with 2-mercaptoethanol. The latter was obtained in 54% yield by the similar treatment of the protected tridecapeptide ester, Z - Pyr - Leu - Tyr - Glu(OBzl) - Asn - Lys(Z) - Pro - Arg(Mts) - Arg(Mts) - Pro - Tyr - Ile - Leu - OBzl, with methanesulfonic acid. As scavenger, a mixture of anisole-thioanisole-o-cresol (1:1:1, by vol.) was employed to suppress the side reaction, O-mesitylene-2-sulfonation of the Tyr residue."}
{"id": "PMID:489256", "title": "Empirical torsional potential functions from protein structure data. Phi- and psi-potentials for non-glycyl amino acid residues.", "content": "The torsional potential functions Vt(phi) and Vt(psi) around single bonds N--C alpha and C alpha--C, which can be used in conformational studies of oligopeptides, polypeptides and proteins, have been derived, using crystal structure data of 22 globular proteins, fitting the observed distribution in the (phi, psi)-plane with the value of Vtot(phi, psi), using the Boltzmann distribution. The averaged torsional potential functions, obtained from various amino acid residues in L-configuration, are Vt(phi) = 1.0 cos (phi + 60 degrees); Vt(psi) = 0.5 cos (psi + 60 degrees) - 1.0 cos (2 psi + 30 degrees) - 0.5 cos (3 psi + 30 degrees). The dipeptide energy maps Vtot(phi, psi) obtained using these functions, instead of the normally accepted torsional functions, were found to explain various observations, such as the absence of the left-handed alpha helix and the C7 conformation, and the relatively high density of points near the line psi = 0 degrees. These functions derived from observational data on protein structures, will, it is hoped, explain various previously unexplained facts in polypeptide conformation.", "contents": "Empirical torsional potential functions from protein structure data. Phi- and psi-potentials for non-glycyl amino acid residues. The torsional potential functions Vt(phi) and Vt(psi) around single bonds N--C alpha and C alpha--C, which can be used in conformational studies of oligopeptides, polypeptides and proteins, have been derived, using crystal structure data of 22 globular proteins, fitting the observed distribution in the (phi, psi)-plane with the value of Vtot(phi, psi), using the Boltzmann distribution. The averaged torsional potential functions, obtained from various amino acid residues in L-configuration, are Vt(phi) = 1.0 cos (phi + 60 degrees); Vt(psi) = 0.5 cos (psi + 60 degrees) - 1.0 cos (2 psi + 30 degrees) - 0.5 cos (3 psi + 30 degrees). The dipeptide energy maps Vtot(phi, psi) obtained using these functions, instead of the normally accepted torsional functions, were found to explain various observations, such as the absence of the left-handed alpha helix and the C7 conformation, and the relatively high density of points near the line psi = 0 degrees. These functions derived from observational data on protein structures, will, it is hoped, explain various previously unexplained facts in polypeptide conformation."}
{"id": "PMID:489257", "title": "Comparative temperature-stability properties of malate dehydrogenases from some thermophilic fungi.", "content": "Temperature-activity and temperature-stability relationships of malate dehydrogenases from eight thermophilic fungi were determined. Temperature optima for maximum activity of the enzymes varied between 50 degrees and 60 degrees and the Arrhenius plots were linear between 5 degrees and 50 degrees. The energies of activation ranged from 2.1 Kcal/mol for the enzyme from Sporotrichum thermophile to 9.1 Kcal/mol for that from Penicillium duponti. Heat inactivation kinetics at 50 degrees revealed heat lability of the enzyme from most of the thermophilic fungi. The t1/2's (min) were less than 10 for the enzymes from P. duponti, S. thermophile and Thermoascus aurantiacus; less than 30 for those from Chaetomium thermophile var. coprophile, H. lanuginosa and C. thermophile var. dissitum; and greater than 30 for those from Mucor pusillus and H. insolens. Salts of Na+, K+ and NH4+, and citrate protected the enzymes from H. lanuginosa, C. thermophile var. dissitum and M. pusillus against heat inactivation.", "contents": "Comparative temperature-stability properties of malate dehydrogenases from some thermophilic fungi. Temperature-activity and temperature-stability relationships of malate dehydrogenases from eight thermophilic fungi were determined. Temperature optima for maximum activity of the enzymes varied between 50 degrees and 60 degrees and the Arrhenius plots were linear between 5 degrees and 50 degrees. The energies of activation ranged from 2.1 Kcal/mol for the enzyme from Sporotrichum thermophile to 9.1 Kcal/mol for that from Penicillium duponti. Heat inactivation kinetics at 50 degrees revealed heat lability of the enzyme from most of the thermophilic fungi. The t1/2's (min) were less than 10 for the enzymes from P. duponti, S. thermophile and Thermoascus aurantiacus; less than 30 for those from Chaetomium thermophile var. coprophile, H. lanuginosa and C. thermophile var. dissitum; and greater than 30 for those from Mucor pusillus and H. insolens. Salts of Na+, K+ and NH4+, and citrate protected the enzymes from H. lanuginosa, C. thermophile var. dissitum and M. pusillus against heat inactivation."}
{"id": "PMID:489259", "title": "On some EEG patterns in children and adults.", "content": "The EEGs of 76 children (6--9 years of age) were classified into five types for the occipital regions (O1 and O2) and into seven types for the central regions (C3 and C4) by the method of EEG pattern discrimination (Sato, Ono et al., 1977). The five types of EEG patterns observed in the occipital regions were: (1) low frequency alpha wave of about 8--9 Hz, (2) Hz alpha wave, (3) low frequency alpha and theta wave, (4) 10 Hz alpha wave and theta wave and (5) irregular wave patterns, while two more types, (6) the low pass and (7) theta wave types, were observed in the central regions. These same types were also observed in the EEGs of 41 adults (20--25 years of age). The amount of difference between the above five and seven types in children and adults were obtained by the generalized (Mahalanobis') distances. In the occipital regions, the percentage frequency of the alpha wave type was the highest followed by that of the alpha and theta wave type, while the frequency of the alpha and theta waves type was the highest in the central regions, followed, next and third in order by the low pass type and the alpha type, respectively.", "contents": "On some EEG patterns in children and adults. The EEGs of 76 children (6--9 years of age) were classified into five types for the occipital regions (O1 and O2) and into seven types for the central regions (C3 and C4) by the method of EEG pattern discrimination (Sato, Ono et al., 1977). The five types of EEG patterns observed in the occipital regions were: (1) low frequency alpha wave of about 8--9 Hz, (2) Hz alpha wave, (3) low frequency alpha and theta wave, (4) 10 Hz alpha wave and theta wave and (5) irregular wave patterns, while two more types, (6) the low pass and (7) theta wave types, were observed in the central regions. These same types were also observed in the EEGs of 41 adults (20--25 years of age). The amount of difference between the above five and seven types in children and adults were obtained by the generalized (Mahalanobis') distances. In the occipital regions, the percentage frequency of the alpha wave type was the highest followed by that of the alpha and theta wave type, while the frequency of the alpha and theta waves type was the highest in the central regions, followed, next and third in order by the low pass type and the alpha type, respectively."}
{"id": "PMID:489261", "title": "The effects of lithium chloride on response to salient and nonsalient stimuli in Carassius auratus.", "content": "The exploratory behaviour of 20 goldfish was investigated to see whether it was affected by treatment with lithium chloride. When familiar and novel stimuli were readily discriminable, lithium treatment had no effect on exploration. In a second study, using 20 fish, it was shown that when novel and familiar stimuli were less markedly different from each other, exploratory responses to the novel stimulus were reduced by lithium. The findings may be related to a general suppressant action of lithium on the extent of stimulus generalisation, or to a reduction by lithium of the animals' responsiveness to near-threshold levels of stimulation.", "contents": "The effects of lithium chloride on response to salient and nonsalient stimuli in Carassius auratus. The exploratory behaviour of 20 goldfish was investigated to see whether it was affected by treatment with lithium chloride. When familiar and novel stimuli were readily discriminable, lithium treatment had no effect on exploration. In a second study, using 20 fish, it was shown that when novel and familiar stimuli were less markedly different from each other, exploratory responses to the novel stimulus were reduced by lithium. The findings may be related to a general suppressant action of lithium on the extent of stimulus generalisation, or to a reduction by lithium of the animals' responsiveness to near-threshold levels of stimulation."}
{"id": "PMID:489262", "title": "Clomipramine and amitriptyline in the treatment of severe pain.", "content": "Clomipramine is the most potent 5-HT reuptake blockade agent among the antidepressants. A comparison between the effect of clomipramine and a less powerful 5-HT reuptake blockade agent (amitriptyline) could test the hypothesis that brain 5-HT is a mediator of pain sensation. Groups of patients of either sex, with pain indication of trigeminal neuralgia, tension headache or postherpatic neuralgia, received doses of clomipramine or amitriptyline in a single blind clinical experiment. The results after three months of treatment showed that clomipramine: (1) was better than amitriptyline in treating trigeminal neuralgia; (2) tended to be better in the treatment of tension headache; and (3) amitriptyline is better in treating postherpatic neuralgia. Clomipramine was better tolerated. The results support the hypothesis that in certain pain situations, clomipramine exerts a beneficial effect, not only because of its effect on the depression and anxiety level of the patient, but also via its effects on the 5-HT brain system.", "contents": "Clomipramine and amitriptyline in the treatment of severe pain. Clomipramine is the most potent 5-HT reuptake blockade agent among the antidepressants. A comparison between the effect of clomipramine and a less powerful 5-HT reuptake blockade agent (amitriptyline) could test the hypothesis that brain 5-HT is a mediator of pain sensation. Groups of patients of either sex, with pain indication of trigeminal neuralgia, tension headache or postherpatic neuralgia, received doses of clomipramine or amitriptyline in a single blind clinical experiment. The results after three months of treatment showed that clomipramine: (1) was better than amitriptyline in treating trigeminal neuralgia; (2) tended to be better in the treatment of tension headache; and (3) amitriptyline is better in treating postherpatic neuralgia. Clomipramine was better tolerated. The results support the hypothesis that in certain pain situations, clomipramine exerts a beneficial effect, not only because of its effect on the depression and anxiety level of the patient, but also via its effects on the 5-HT brain system."}
{"id": "PMID:489263", "title": "Changes in hippocampal neuronal nuclei in response to environmental stimulation.", "content": "The diameter of cortical neuronal nuclei has been reported to increase when animals are reared in enriched, as opposed to deprived, sensory environments. The present study investigated whether analogous neuronal changes occur in the hippocampus. Male Wistar rats were reared in either an enriched or a deprived environment for 80 days after weaning. At the completion of this rearing period both brain weights and hippocampal neuronal nuclear diameters were measured. The enriched subjects exhibited a greater forebrain:hindbrain ratio (3.7% p less than 0.05) when compared to the isolated subjects. No significant differences in mean hippocampal nuclear dimensions could be detected but variability in nuclear size was greater in the stratum granulosum of the isolated subjects (p less than 0.01). It is proposed that this increased variability reflects an environmentally restricted development of these neurons.", "contents": "Changes in hippocampal neuronal nuclei in response to environmental stimulation. The diameter of cortical neuronal nuclei has been reported to increase when animals are reared in enriched, as opposed to deprived, sensory environments. The present study investigated whether analogous neuronal changes occur in the hippocampus. Male Wistar rats were reared in either an enriched or a deprived environment for 80 days after weaning. At the completion of this rearing period both brain weights and hippocampal neuronal nuclear diameters were measured. The enriched subjects exhibited a greater forebrain:hindbrain ratio (3.7% p less than 0.05) when compared to the isolated subjects. No significant differences in mean hippocampal nuclear dimensions could be detected but variability in nuclear size was greater in the stratum granulosum of the isolated subjects (p less than 0.01). It is proposed that this increased variability reflects an environmentally restricted development of these neurons."}
{"id": "PMID:489264", "title": "Opiate stimulation increases exploration in the mouse.", "content": "Adult male Swiss-Webster mice were injected with a low dose of morphine sulfate or vehicle and placed in an apparatus which allowed the assessment of exploratory behavior. Two measures of exploration (rearing, nose poking) were recorded during a single brief exposure. Both exploratory behaviors increased after drug treatment. This suggests opiate involvement in behavioral arousal, and more specifically, in exploratory behavior.", "contents": "Opiate stimulation increases exploration in the mouse. Adult male Swiss-Webster mice were injected with a low dose of morphine sulfate or vehicle and placed in an apparatus which allowed the assessment of exploratory behavior. Two measures of exploration (rearing, nose poking) were recorded during a single brief exposure. Both exploratory behaviors increased after drug treatment. This suggests opiate involvement in behavioral arousal, and more specifically, in exploratory behavior."}
{"id": "PMID:489265", "title": "Changes in event related potentials during processing of Stroop stimuli.", "content": "In a simplified Stroop test, subjects responded with a button press to either the color or word dimension of Stroop stimuli. Response times were analyzed as a function of condition (color or word), stimulus type (congruent or noncongruent color-word pairing) and visual field of presentation (left or right). Changes in the stimulus evoked potential (Experiment I) and the preresponse (premotor) potential (Experiment II) were measured as a function of the independent variables and hemisphere of recording site. Stimulus type had the expected Stroop effect on response time, with noncongruent stimuli associated with longer RTs. The pattern of changes in the event related potentials suggested that the typical Stroop interference effect (color condition) is localized in the response selection stage of processing. However, the less common reverse Stroop effect (word condition) probably results from more complex processing interactions during encoding of the color and word dimensions.", "contents": "Changes in event related potentials during processing of Stroop stimuli. In a simplified Stroop test, subjects responded with a button press to either the color or word dimension of Stroop stimuli. Response times were analyzed as a function of condition (color or word), stimulus type (congruent or noncongruent color-word pairing) and visual field of presentation (left or right). Changes in the stimulus evoked potential (Experiment I) and the preresponse (premotor) potential (Experiment II) were measured as a function of the independent variables and hemisphere of recording site. Stimulus type had the expected Stroop effect on response time, with noncongruent stimuli associated with longer RTs. The pattern of changes in the event related potentials suggested that the typical Stroop interference effect (color condition) is localized in the response selection stage of processing. However, the less common reverse Stroop effect (word condition) probably results from more complex processing interactions during encoding of the color and word dimensions."}
{"id": "PMID:489266", "title": "Habituation and transfer during sleep in cats.", "content": "In order to test the concept of \"sleep learning\", cats were exposed to habituation treatment of auditory stimuli during different stages of the sleep-waking cycle. While it was possible to demonstrate that habituation to an auditory stimulus can take place in paradoxical sleep (PS) and slow-wave sleep (SWS) as well as during periods of wakefulness, it was not always possible to demonstrate a transfer of habituation from the training period to other periods. A complete transfer of habituation occurred between the two sleep periods (PS and SWS); a partial transfer of habituation occurred between the waking period and both of the two sleep stages (PS and SWS), but only a minimal transfer of habituation was found between any one of the sleep stages and the waking period. When the cats were able to transfer the habituation, they also were able to discriminate between the habituating tone and a novel tone. The findings that only minimal transfer of habituation could occur between each of the sleep stages and the waking period do not lend support to the concept of \"sleep learning\".", "contents": "Habituation and transfer during sleep in cats. In order to test the concept of \"sleep learning\", cats were exposed to habituation treatment of auditory stimuli during different stages of the sleep-waking cycle. While it was possible to demonstrate that habituation to an auditory stimulus can take place in paradoxical sleep (PS) and slow-wave sleep (SWS) as well as during periods of wakefulness, it was not always possible to demonstrate a transfer of habituation from the training period to other periods. A complete transfer of habituation occurred between the two sleep periods (PS and SWS); a partial transfer of habituation occurred between the waking period and both of the two sleep stages (PS and SWS), but only a minimal transfer of habituation was found between any one of the sleep stages and the waking period. When the cats were able to transfer the habituation, they also were able to discriminate between the habituating tone and a novel tone. The findings that only minimal transfer of habituation could occur between each of the sleep stages and the waking period do not lend support to the concept of \"sleep learning\"."}
{"id": "PMID:489268", "title": "Absolute lung density in experimental canine pulmonary edema.", "content": "The noninvasive quantification of pulmonary edema could be of importance in patient management. We have developed a portable Compton-scatter densitometer capable of measuring density in the range of 0.1-1 g/cm3 independently of the chest wall. Change in lung density was investigated in 19 dogs with pulmonary edema induced by a combination of raised left atrial pressure and hemodilution. The increase in lung density correlated with the postmortem assessment of lung water (r = 0.862, p less than 0.01) and with hemodynamic indicators of pulmonary edema (r = 0.749, p less than 0.01). Subtracting the intravascular component of lung density did not improve the correlation with postmortem lung water (r = 0.850, p less than 0.01). The measurement of absolute lung density by a Compton-scatter technique may be a potentially useful method of quantifying pulmonary edema.", "contents": "Absolute lung density in experimental canine pulmonary edema. The noninvasive quantification of pulmonary edema could be of importance in patient management. We have developed a portable Compton-scatter densitometer capable of measuring density in the range of 0.1-1 g/cm3 independently of the chest wall. Change in lung density was investigated in 19 dogs with pulmonary edema induced by a combination of raised left atrial pressure and hemodilution. The increase in lung density correlated with the postmortem assessment of lung water (r = 0.862, p less than 0.01) and with hemodynamic indicators of pulmonary edema (r = 0.749, p less than 0.01). Subtracting the intravascular component of lung density did not improve the correlation with postmortem lung water (r = 0.850, p less than 0.01). The measurement of absolute lung density by a Compton-scatter technique may be a potentially useful method of quantifying pulmonary edema."}
{"id": "PMID:489269", "title": "Serioangiographic study of renal cortical necrosis induced by administration of estrin and vasopressin in rats.", "content": "We report a serioangiographic method in rats which permits assessment of the course and dimensions of the renal arteries, the durations of the arterial and venous phases, and the intensity and uniformity of the renal parenchymal filling. The procedure was employed to study the mechanism by which administration of vasopressin to rats pretreated with estrin leads to renal cortical necrosis. The pathogenetic significance of the spasm localized on the larger renal arteries was proved directly; the possible role of the arteriovenous shunt in the development of the renal ischemia was excluded.", "contents": "Serioangiographic study of renal cortical necrosis induced by administration of estrin and vasopressin in rats. We report a serioangiographic method in rats which permits assessment of the course and dimensions of the renal arteries, the durations of the arterial and venous phases, and the intensity and uniformity of the renal parenchymal filling. The procedure was employed to study the mechanism by which administration of vasopressin to rats pretreated with estrin leads to renal cortical necrosis. The pathogenetic significance of the spasm localized on the larger renal arteries was proved directly; the possible role of the arteriovenous shunt in the development of the renal ischemia was excluded."}
{"id": "PMID:489270", "title": "The blood-brain barrier: effects of nonionic contrast media with and without addition of Ca2+ and Mg2+.", "content": "Trypan blue and 197HgCl2 were used as markers of the increased leakage through the blood-brain barrier which developed after selective injection of different contrast media formulations into the internal carotid arteries of rabbits. Interest was focused on a new, experimental, nonionic medium (C-29) and possible effects resulting from the addition of Ca2+ and Mg2+ to nonionic contrast media. Using 197Hg, it was possible to quantitate the increase in extravasation produced by the contrast media. C-29 caused less damage than either metrizamide or meglumine iothalamate. The addition of Ca2+ and Mg2+ to nonionic contrast media seemed to stabilize the blood-brain barrier.", "contents": "The blood-brain barrier: effects of nonionic contrast media with and without addition of Ca2+ and Mg2+. Trypan blue and 197HgCl2 were used as markers of the increased leakage through the blood-brain barrier which developed after selective injection of different contrast media formulations into the internal carotid arteries of rabbits. Interest was focused on a new, experimental, nonionic medium (C-29) and possible effects resulting from the addition of Ca2+ and Mg2+ to nonionic contrast media. Using 197Hg, it was possible to quantitate the increase in extravasation produced by the contrast media. C-29 caused less damage than either metrizamide or meglumine iothalamate. The addition of Ca2+ and Mg2+ to nonionic contrast media seemed to stabilize the blood-brain barrier."}
{"id": "PMID:489271", "title": "Cardiovascular effects induced by the injection of a new nonionic contrast medium (Iopamidol): experimental study in dogs.", "content": "The hemodynamic effects induced by the injection in the pulmonary artery of the new nonionic water soluble contrast medium Iopamidol were compared with those obtained by the injection of two other currently used contrast media (meglumine diatrizoate and sodium iothalamate). The experiments were carried out in nine mongrel dogs. Hemodynamic variables were continuously measured prior to, during, and for 8 minutes after injection of the contrast media. Injections of iopamidol produced significantly smaller decreases in aortic pressure (p less than 0.01), contractile indices (p less than 0.01), and peripheral resistances (p less than 0.01), and changes in heart rate and in cardiac output were less pronounced. At 3-4 minutes after injection, an increase in Vmaxd was observed with all three contrast media, but it was significantly lower after injecting Iopamidol. The role of hyperosmolality in causing cardiovascular changes is discussed. The less significant changes induced by Iopamidol appear to be the result of its lower osmolality, which is about a third that of meglumine diatrizoate or sodium iothalamate.", "contents": "Cardiovascular effects induced by the injection of a new nonionic contrast medium (Iopamidol): experimental study in dogs. The hemodynamic effects induced by the injection in the pulmonary artery of the new nonionic water soluble contrast medium Iopamidol were compared with those obtained by the injection of two other currently used contrast media (meglumine diatrizoate and sodium iothalamate). The experiments were carried out in nine mongrel dogs. Hemodynamic variables were continuously measured prior to, during, and for 8 minutes after injection of the contrast media. Injections of iopamidol produced significantly smaller decreases in aortic pressure (p less than 0.01), contractile indices (p less than 0.01), and peripheral resistances (p less than 0.01), and changes in heart rate and in cardiac output were less pronounced. At 3-4 minutes after injection, an increase in Vmaxd was observed with all three contrast media, but it was significantly lower after injecting Iopamidol. The role of hyperosmolality in causing cardiovascular changes is discussed. The less significant changes induced by Iopamidol appear to be the result of its lower osmolality, which is about a third that of meglumine diatrizoate or sodium iothalamate."}
{"id": "PMID:489273", "title": "Isolated microperfused tubule model to evaluate contrast media transport in normal and occluded renal tubule segments.", "content": "We evaluated tubular handling of urographic contrast media in normal and obstructed states by the use of a model which allowed direct evaluation of transcellular flux. Segments of rabbit renal tubule were harvested by microdissection. Individual tubular segments were cannulated by micropipette while immersed in a serum bath. Radioactive iodine-labeled sodium iothalamate was added to the perfusate of normal and distally obstructed nephron segments. Any iothalamate that moved through the tubule wall was recovered in the bath. The tubular epithelium was counted to determine epithelial concentration of iothalamate. A small efflux of contrast medium occurred which was unaffected by acute obstruction and increased pressure. Proximal straight tubules were more permeable to sodium iothalamate than cortical collecting tubules.", "contents": "Isolated microperfused tubule model to evaluate contrast media transport in normal and occluded renal tubule segments. We evaluated tubular handling of urographic contrast media in normal and obstructed states by the use of a model which allowed direct evaluation of transcellular flux. Segments of rabbit renal tubule were harvested by microdissection. Individual tubular segments were cannulated by micropipette while immersed in a serum bath. Radioactive iodine-labeled sodium iothalamate was added to the perfusate of normal and distally obstructed nephron segments. Any iothalamate that moved through the tubule wall was recovered in the bath. The tubular epithelium was counted to determine epithelial concentration of iothalamate. A small efflux of contrast medium occurred which was unaffected by acute obstruction and increased pressure. Proximal straight tubules were more permeable to sodium iothalamate than cortical collecting tubules."}
{"id": "PMID:489396", "title": "[Indications for surgery in adrenocortical disease].", "content": "The surgical treatment of adrenocortical diseases indicated for hormone-secreting neoplasms and for definite cases of Cushing's disease. The surgical indication will be discussed. Since primary hyperaldosteronism caused by nodular hyperplasia of the adrenals needs a medical rather than a surgical treatment, a preoperative diagnosis and location of aldosterone producing adenomas has to be performed.", "contents": "[Indications for surgery in adrenocortical disease]. The surgical treatment of adrenocortical diseases indicated for hormone-secreting neoplasms and for definite cases of Cushing's disease. The surgical indication will be discussed. Since primary hyperaldosteronism caused by nodular hyperplasia of the adrenals needs a medical rather than a surgical treatment, a preoperative diagnosis and location of aldosterone producing adenomas has to be performed."}
{"id": "PMID:489397", "title": "[Adrenal scintigraphy-value and indications in adrenal disease].", "content": "Adrenal scintillation scanning is a very useful method in the diagnosis of steroid producing diseases of the adrenal glands. The diagnosis of bilateral hyperplasia and primary adenoma of the adrenal cortex in Cushing's syndrome and Conn's syndrome is possible by this non-invasive procedure. Morphological questions (e.g. the localization of a phaeochromocytoma) cannot be answered. It is not a screening examination.", "contents": "[Adrenal scintigraphy-value and indications in adrenal disease]. Adrenal scintillation scanning is a very useful method in the diagnosis of steroid producing diseases of the adrenal glands. The diagnosis of bilateral hyperplasia and primary adenoma of the adrenal cortex in Cushing's syndrome and Conn's syndrome is possible by this non-invasive procedure. Morphological questions (e.g. the localization of a phaeochromocytoma) cannot be answered. It is not a screening examination."}
{"id": "PMID:489398", "title": "[Medical support in surgery for pheochromocytoma].", "content": "The occurrence of hypertensive crises, arrhythmias and hypotensive crises during surgery for pheochromocytoma can be prevented both preoperatively and intraoperatively by adequate alpha-adrenergic blockade and volume replacement. Only in a few cases is additional beta-adrenergic blockade necessary. Preoperatively the author uses phenoxybenzamine (Dibenicyline) as alpha-blockade in a dose of 20-30 mg daily, and propanolol (Inderal) as beta-blockade in a dose of 4 X 20 mg daily, both given orally. Intraoperatively he gives preference to alpha-adrenergic receptor blockers with a short halflife, i.e. phentolamine (Regitin) and lidocaine or pranolol as the beta-adrenergic blocker.", "contents": "[Medical support in surgery for pheochromocytoma]. The occurrence of hypertensive crises, arrhythmias and hypotensive crises during surgery for pheochromocytoma can be prevented both preoperatively and intraoperatively by adequate alpha-adrenergic blockade and volume replacement. Only in a few cases is additional beta-adrenergic blockade necessary. Preoperatively the author uses phenoxybenzamine (Dibenicyline) as alpha-blockade in a dose of 20-30 mg daily, and propanolol (Inderal) as beta-blockade in a dose of 4 X 20 mg daily, both given orally. Intraoperatively he gives preference to alpha-adrenergic receptor blockers with a short halflife, i.e. phentolamine (Regitin) and lidocaine or pranolol as the beta-adrenergic blocker."}
{"id": "PMID:489399", "title": "[Incidence of adrenal metastasis from renal carcinoma].", "content": "We analysed the cases of renal carcinoma undergoing operation or autopsy from 1970 to 1978 in respect of their metastasizing to the adrenals. Of the 31 cases undergoing autopsy 10 adrenal metastases were found mostly as a result of a widespread metastasis. Of the operated 74 patients 37 underwent nephrectomy alone and 37 nephrectomy together with prophylactic adrenalectomy. The histologic evaluation of the 37 removed adrenals revealed metastases only in 4 cases. Only one of them proved to be a single metastasis, the other occurring together with other metastatic lesions. The value of the adrenalectomy will be discussed.", "contents": "[Incidence of adrenal metastasis from renal carcinoma]. We analysed the cases of renal carcinoma undergoing operation or autopsy from 1970 to 1978 in respect of their metastasizing to the adrenals. Of the 31 cases undergoing autopsy 10 adrenal metastases were found mostly as a result of a widespread metastasis. Of the operated 74 patients 37 underwent nephrectomy alone and 37 nephrectomy together with prophylactic adrenalectomy. The histologic evaluation of the 37 removed adrenals revealed metastases only in 4 cases. Only one of them proved to be a single metastasis, the other occurring together with other metastatic lesions. The value of the adrenalectomy will be discussed."}
{"id": "PMID:489400", "title": "[Long-term observations after adrenal surgery].", "content": "Long-term observations after adrenal surgery show different results according to indications for operations. The longest period of observation shows 8 patients with adrenal hyperplasia. In 3 of 4 bilaterally adrenalectomised patients no satisfactory results were obtained despite substitution treatment; 4 one-side adrenalectomised patients are in good condition. Operative results of 7 patients with benign phaeochromocytoma are satisfactory. Two patients with malignant phaeochromocytoma developed a local recidive and distant metastases. Carcinoma of the adrenal gland, if not hormonally active, were not diagnosed in early stage, 3 patients died from metastases within 3 years. A 2-years-old girl, who was operated on with hormonally active carcinoma, is in good condition 21 years later.", "contents": "[Long-term observations after adrenal surgery]. Long-term observations after adrenal surgery show different results according to indications for operations. The longest period of observation shows 8 patients with adrenal hyperplasia. In 3 of 4 bilaterally adrenalectomised patients no satisfactory results were obtained despite substitution treatment; 4 one-side adrenalectomised patients are in good condition. Operative results of 7 patients with benign phaeochromocytoma are satisfactory. Two patients with malignant phaeochromocytoma developed a local recidive and distant metastases. Carcinoma of the adrenal gland, if not hormonally active, were not diagnosed in early stage, 3 patients died from metastases within 3 years. A 2-years-old girl, who was operated on with hormonally active carcinoma, is in good condition 21 years later."}
{"id": "PMID:489401", "title": "[Conservative surgery of kidney adrenoma].", "content": "Adenomas of the kidney are often tumors of doubtful dignity because the histopathological criteria are unclear and, moreover, the preoperative diagnosis lacks complete reliability. As a result, the clinician frequently has problems deciding on conservative surgery. This is demonstrated in the light of 6 cases of renal adenoma. The surgical management of these tumors depends on exact classification of their dignity by the pathologist. Proposals on the subject are put forward and discussed.", "contents": "[Conservative surgery of kidney adrenoma]. Adenomas of the kidney are often tumors of doubtful dignity because the histopathological criteria are unclear and, moreover, the preoperative diagnosis lacks complete reliability. As a result, the clinician frequently has problems deciding on conservative surgery. This is demonstrated in the light of 6 cases of renal adenoma. The surgical management of these tumors depends on exact classification of their dignity by the pathologist. Proposals on the subject are put forward and discussed."}
{"id": "PMID:489402", "title": "[Conservative surgery of renal pelvic tumors].", "content": "In a large series of renal pelvic and ureteral tumors in Belgrade (Jugoslavia), 26 cases of renal pelvic tumors were operated with conservation of the kidney. The indications must be strictly observed, the possibility of a relapse being maximally excluded. The results, under strict indications, are quite well: from 26 operated cases the 12 from 16 followed longer that 5 years are alive and with good kidney function plus 1 with lost kidney. So the 74% success for 5 or more years completely justifies the conservation of the kidney. In other cases the nephroureterectomy must be practised. The author did in 188 cases.", "contents": "[Conservative surgery of renal pelvic tumors]. In a large series of renal pelvic and ureteral tumors in Belgrade (Jugoslavia), 26 cases of renal pelvic tumors were operated with conservation of the kidney. The indications must be strictly observed, the possibility of a relapse being maximally excluded. The results, under strict indications, are quite well: from 26 operated cases the 12 from 16 followed longer that 5 years are alive and with good kidney function plus 1 with lost kidney. So the 74% success for 5 or more years completely justifies the conservation of the kidney. In other cases the nephroureterectomy must be practised. The author did in 188 cases."}
{"id": "PMID:489403", "title": "[Cytodiagnosis of pathologic changes in the renal pelvis and ureter].", "content": "In 80 cases of roentgenologic filling defects in renal pelvis and ureter, which could be determined exactly preoperatively, we could confirm the diagnostic value of cytology from washings of renal pelvis and ureter (lavage cytology) especially in preoperative diagnosis of carcinoma of renal pelvis and ureter. Twenty of 25 tumors (80%) were detected preoperatively. There were no false positive results. The diagnostic reliability of this method was more accurate than with normal exfoliative urinary cytology from other authors. The results are discussed together with the literature.", "contents": "[Cytodiagnosis of pathologic changes in the renal pelvis and ureter]. In 80 cases of roentgenologic filling defects in renal pelvis and ureter, which could be determined exactly preoperatively, we could confirm the diagnostic value of cytology from washings of renal pelvis and ureter (lavage cytology) especially in preoperative diagnosis of carcinoma of renal pelvis and ureter. Twenty of 25 tumors (80%) were detected preoperatively. There were no false positive results. The diagnostic reliability of this method was more accurate than with normal exfoliative urinary cytology from other authors. The results are discussed together with the literature."}
{"id": "PMID:489405", "title": "[Surgical considerations in congenital hydronephrosis].", "content": "The anatomo-operative characteristics of the ischemic stenosis of the juxtapyelic part of the ureter, its embryogenic fundamentals, clinical semiology and urography, emphasizing the signs of the latter for the differential diagnosis of hydronephrosis are presented. The most adequate surgical technique, that is the extirpation of the scleroatrophic part of the ureter, followed by uretero-pyelostomy, with special care in preserving the arteries of the ureter, is summarized and the technique for the postoperative drainage of the kidney is pointed out. The poor effectiveness of other procedures are commented.", "contents": "[Surgical considerations in congenital hydronephrosis]. The anatomo-operative characteristics of the ischemic stenosis of the juxtapyelic part of the ureter, its embryogenic fundamentals, clinical semiology and urography, emphasizing the signs of the latter for the differential diagnosis of hydronephrosis are presented. The most adequate surgical technique, that is the extirpation of the scleroatrophic part of the ureter, followed by uretero-pyelostomy, with special care in preserving the arteries of the ureter, is summarized and the technique for the postoperative drainage of the kidney is pointed out. The poor effectiveness of other procedures are commented."}
{"id": "PMID:489406", "title": "[Extraction of ureteral stones using a catheter with 2 inflatable balloons. Preliminary report].", "content": "In a preliminary communication a new method for endoluminal extraction of ureteral stones is described by using a catheter with two inflatable balloons. The length of the catheter is about 100 cm and its diameter corresponds to the usual ureteral catheters. It has four openings: the central opening is located at the end of the catheters. Two other lumina open into the two balloons located at a distance of 3-5 cm. An other opening is located between the two balloons. By insufflating and emptying of the distal balloon, the concrement in the ureter is mobilized and caught. By insufflating the two balloons and injection of contrast media through the opening situated between the two balloons, the ureter is ovistended, the stone is mobilized can be extrac \"swimming\" ie contrast media between the two balloons. Tillenow, o concrements have been extracted from the distal ureter by this method.", "contents": "[Extraction of ureteral stones using a catheter with 2 inflatable balloons. Preliminary report]. In a preliminary communication a new method for endoluminal extraction of ureteral stones is described by using a catheter with two inflatable balloons. The length of the catheter is about 100 cm and its diameter corresponds to the usual ureteral catheters. It has four openings: the central opening is located at the end of the catheters. Two other lumina open into the two balloons located at a distance of 3-5 cm. An other opening is located between the two balloons. By insufflating and emptying of the distal balloon, the concrement in the ureter is mobilized and caught. By insufflating the two balloons and injection of contrast media through the opening situated between the two balloons, the ureter is ovistended, the stone is mobilized can be extrac \"swimming\" ie contrast media between the two balloons. Tillenow, o concrements have been extracted from the distal ureter by this method."}
{"id": "PMID:489407", "title": "[Surveillance of 110 patients after ureterolithotomy].", "content": "Between 1971 and 1975 we controlled 110 patients after ureterolithotomy. Early complications were one nephrectomy and two wound-infections. As late complications we saw one partial stenosis of the ureter. Our results show that ureterolithotomy is a low risk intervention.", "contents": "[Surveillance of 110 patients after ureterolithotomy]. Between 1971 and 1975 we controlled 110 patients after ureterolithotomy. Early complications were one nephrectomy and two wound-infections. As late complications we saw one partial stenosis of the ureter. Our results show that ureterolithotomy is a low risk intervention."}
{"id": "PMID:489408", "title": "[Ureterocystoneostomy using the Politano-Leadbetter procedure in adults].", "content": "At the Urology Clinic of the University of Berne during a seven-year period 79 ureterocystoneostomies (UCNS) have been performed with adults suffering from various urologic illness. Technique of operation was a modified Politano-Leadbetter procedure. Over 90% success was achieved in the reflux-only group, acceptable results were obtained with patients suffering from ureteral problems due to gynecologic surgical injury or tuberculosis, while reimplantation of megaureters had a low success rate. Early postoperative complications were rare, and mortality due to the operation was zero.", "contents": "[Ureterocystoneostomy using the Politano-Leadbetter procedure in adults]. At the Urology Clinic of the University of Berne during a seven-year period 79 ureterocystoneostomies (UCNS) have been performed with adults suffering from various urologic illness. Technique of operation was a modified Politano-Leadbetter procedure. Over 90% success was achieved in the reflux-only group, acceptable results were obtained with patients suffering from ureteral problems due to gynecologic surgical injury or tuberculosis, while reimplantation of megaureters had a low success rate. Early postoperative complications were rare, and mortality due to the operation was zero."}
{"id": "PMID:489409", "title": "[Results of corrective surgery of hydronephrosis].", "content": "The analysis of 120 cases of hydronephrosis and 124 of corrective operations of hydronephrosis (4 bilaterally) from the Urological Clinic in Belgrade, with 81.5% very good and good results, showed the endresult which is dependent from the correctness of the indication for surgical corrective treatment, adequacy of the performed type of the corrective operation, the correctness of its performance, lack of complications and its abolishment even more from anatomical resp. functional state of the kidney before the operation. Less damaged kidneys precede well, and developed damage has a bad prognosis of the corrective operation.", "contents": "[Results of corrective surgery of hydronephrosis]. The analysis of 120 cases of hydronephrosis and 124 of corrective operations of hydronephrosis (4 bilaterally) from the Urological Clinic in Belgrade, with 81.5% very good and good results, showed the endresult which is dependent from the correctness of the indication for surgical corrective treatment, adequacy of the performed type of the corrective operation, the correctness of its performance, lack of complications and its abolishment even more from anatomical resp. functional state of the kidney before the operation. Less damaged kidneys precede well, and developed damage has a bad prognosis of the corrective operation."}
{"id": "PMID:489410", "title": "[Experiences with instrumental methods for urinary calculi analysis].", "content": "To reduce the urinary calculi incidence by calculi formers, it is important to know the composition of these stones. Unfortunately the chemical analysis does not give very reliable results. Looking for a better method to analyse urinary calculi, three instrumental methods were tested: infared spectroscopy, thermal analysis and X-ray diffraction. The experimental results and economical considerations show that the X-ray diffraction analysis of urinary calculi would meet the goal of improved care of patients with stones.", "contents": "[Experiences with instrumental methods for urinary calculi analysis]. To reduce the urinary calculi incidence by calculi formers, it is important to know the composition of these stones. Unfortunately the chemical analysis does not give very reliable results. Looking for a better method to analyse urinary calculi, three instrumental methods were tested: infared spectroscopy, thermal analysis and X-ray diffraction. The experimental results and economical considerations show that the X-ray diffraction analysis of urinary calculi would meet the goal of improved care of patients with stones."}
{"id": "PMID:489411", "title": "[New diagnostic examinations in patients with urinary calculi, taking into consideration primary hyperparathyroidism].", "content": "The serum levels of total calcium and anorganic phosphate have been the leading parameters in the diagnosis of primary hyperparathyroidism. In addition to these, it is now possible to measure ionized calcium (Ca++) by an ion-selective electrode and parathormone (PTH) by C- and N-terminal radioimmunoassays (RIA). Whereas Ca++ determination and C-terminal PTH RIA represent a diagnostic progress, this can not be claimed for the N-terminal PTH RIA in peripheral venous blood especially in border-line cases.", "contents": "[New diagnostic examinations in patients with urinary calculi, taking into consideration primary hyperparathyroidism]. The serum levels of total calcium and anorganic phosphate have been the leading parameters in the diagnosis of primary hyperparathyroidism. In addition to these, it is now possible to measure ionized calcium (Ca++) by an ion-selective electrode and parathormone (PTH) by C- and N-terminal radioimmunoassays (RIA). Whereas Ca++ determination and C-terminal PTH RIA represent a diagnostic progress, this can not be claimed for the N-terminal PTH RIA in peripheral venous blood especially in border-line cases."}
{"id": "PMID:489412", "title": "[Clinical importance of vesico-renal reflux after transurethral resection of bladder tumors].", "content": "After any resection of tumors near the ostium you have to expect the vesicoureteral reflux as a complication. If the upper urinary tract is primarily normal, this reflux is of no clinical importance (if of course it is diagnosed and controlled). The resection of the ostium, to be radical, can be done without any doubt. The upper urinary tract is rarely hurted. Concerning out patients it is impossible to see how often the tumor spread in the upper urinary tract by the reflux. However, one must consider it, as one of our cases shows.", "contents": "[Clinical importance of vesico-renal reflux after transurethral resection of bladder tumors]. After any resection of tumors near the ostium you have to expect the vesicoureteral reflux as a complication. If the upper urinary tract is primarily normal, this reflux is of no clinical importance (if of course it is diagnosed and controlled). The resection of the ostium, to be radical, can be done without any doubt. The upper urinary tract is rarely hurted. Concerning out patients it is impossible to see how often the tumor spread in the upper urinary tract by the reflux. However, one must consider it, as one of our cases shows."}
{"id": "PMID:489413", "title": "[Experiences with a new method of surgery for incontinence].", "content": "Our new operation method against the prostatectomy-incontinence is only indicated, if the external urethral sphincter is intact. This possible in 90% of all incontinent patients after prostatectomy. In this situation we can guarantee a good chance of success. An interoperative lesion of the bulbi arteries, which enter in the urethra through the posterior portion of the bulbocavernosus muscle, must be prevented. A lesion of them leeds to a total necrosis of the posterior urethra.", "contents": "[Experiences with a new method of surgery for incontinence]. Our new operation method against the prostatectomy-incontinence is only indicated, if the external urethral sphincter is intact. This possible in 90% of all incontinent patients after prostatectomy. In this situation we can guarantee a good chance of success. An interoperative lesion of the bulbi arteries, which enter in the urethra through the posterior portion of the bulbocavernosus muscle, must be prevented. A lesion of them leeds to a total necrosis of the posterior urethra."}
{"id": "PMID:489414", "title": "[A device for surgery of phimosis in adults].", "content": "A simple device for circumcision in adolescents and adults is described. It is suitable for all kinds of too tight foreskin. In 150 patients we did not see a postoperative hemorrhage or recurrence of the phimosis.", "contents": "[A device for surgery of phimosis in adults]. A simple device for circumcision in adolescents and adults is described. It is suitable for all kinds of too tight foreskin. In 150 patients we did not see a postoperative hemorrhage or recurrence of the phimosis."}
{"id": "PMID:489415", "title": "[Treatment of biliary tract carcinoma with metal endoprosthesis].", "content": "The application of endoprosthesis in the surgery of biliary tract carcinoma is a very valuable method to prolong the life of cancer patients. The metalendoprosthesis with drain for continuously rinsing the bile duct to avoid incrusation showed good results in 9 patients for short survival time of 5-26 months. Two patients were treated with bifurcated Y-shape prosthesis after resection of bile duct carcinoma.", "contents": "[Treatment of biliary tract carcinoma with metal endoprosthesis]. The application of endoprosthesis in the surgery of biliary tract carcinoma is a very valuable method to prolong the life of cancer patients. The metalendoprosthesis with drain for continuously rinsing the bile duct to avoid incrusation showed good results in 9 patients for short survival time of 5-26 months. Two patients were treated with bifurcated Y-shape prosthesis after resection of bile duct carcinoma."}
{"id": "PMID:489416", "title": "[Conservative treatment of tibial fractures using Sarmiento's functional technic].", "content": "A conservative functional technique of treatment of tibial fractures is described. This method allows the mobilization of knee and ankle joints and early weight-bearing. The leg is fitted into an Orthoplast orthosis fixed to a plastic sole which permits the wearing of shoe and sock. The experience in 56 patients is described. Excellent function results obtained after a relatively short time recommend this type of treatment.", "contents": "[Conservative treatment of tibial fractures using Sarmiento's functional technic]. A conservative functional technique of treatment of tibial fractures is described. This method allows the mobilization of knee and ankle joints and early weight-bearing. The leg is fitted into an Orthoplast orthosis fixed to a plastic sole which permits the wearing of shoe and sock. The experience in 56 patients is described. Excellent function results obtained after a relatively short time recommend this type of treatment."}
{"id": "PMID:489417", "title": "[Clinical considerations on posttraumatic osteitis].", "content": "Posttraumatic bone infection very often signifies a life threatening complication. However, it must no longer be accepted that amputation of the afflicted limb be the logical treatment of such a complication. A surgeon who offers the advantages of operative treatment of a fracture to his patient must also be able to control its complications. Osteitis can be treated with a good chance of success. In our clinic 29 bone infections were treated, 13 of them healing without substantial restrictions, 7 with distinct discomfort and 4 with arthrodesis, i.e. 83% healed. However, the treatment may require months and may necessitate further operative interventions. The transfer of such a patient from an acute care hospital to a high altitude clinic offers -- besides the medical and psychological advantage of a continuous long-term treatment plan -- the climatic conditions of sun and high altitude air which apparently act very favorably on the healing process of chronic infections. Of course, the possibility of competent surgical interventions must exist. Thus, with proper therapy the disastrous chain of errors beginning mainly with poor initial care through insufficient treatment can be broken.", "contents": "[Clinical considerations on posttraumatic osteitis]. Posttraumatic bone infection very often signifies a life threatening complication. However, it must no longer be accepted that amputation of the afflicted limb be the logical treatment of such a complication. A surgeon who offers the advantages of operative treatment of a fracture to his patient must also be able to control its complications. Osteitis can be treated with a good chance of success. In our clinic 29 bone infections were treated, 13 of them healing without substantial restrictions, 7 with distinct discomfort and 4 with arthrodesis, i.e. 83% healed. However, the treatment may require months and may necessitate further operative interventions. The transfer of such a patient from an acute care hospital to a high altitude clinic offers -- besides the medical and psychological advantage of a continuous long-term treatment plan -- the climatic conditions of sun and high altitude air which apparently act very favorably on the healing process of chronic infections. Of course, the possibility of competent surgical interventions must exist. Thus, with proper therapy the disastrous chain of errors beginning mainly with poor initial care through insufficient treatment can be broken."}
{"id": "PMID:489418", "title": "[Etiology and prevention of pelvic injuries in traffic accidents].", "content": "With hospitalized traffic accident victimes fractures of the pelvis are not very frequent (4-10%). Fatal cases show a higher fracture incidence (20-25%). Pedestrains are with regard to pelvic fractures twice as frequently affected as other road users. Pedestrians over 60 years of age are most often involved. Modifications of the car front structure are expected to lead to significant improvements for the unprotected road user. Seat belts are capable of reducing pelvic fracture with car occupants only in certain frontal collisions. However, the geometry of the lap belt portion is of importance for the overall effectiveness of the belt system.", "contents": "[Etiology and prevention of pelvic injuries in traffic accidents]. With hospitalized traffic accident victimes fractures of the pelvis are not very frequent (4-10%). Fatal cases show a higher fracture incidence (20-25%). Pedestrains are with regard to pelvic fractures twice as frequently affected as other road users. Pedestrians over 60 years of age are most often involved. Modifications of the car front structure are expected to lead to significant improvements for the unprotected road user. Seat belts are capable of reducing pelvic fracture with car occupants only in certain frontal collisions. However, the geometry of the lap belt portion is of importance for the overall effectiveness of the belt system."}
{"id": "PMID:489419", "title": "Combined histochemical and biochemical investigation to the reliability of the demonstration of arylsulphatase activity in cryostat sections.", "content": "The reliability of the enzyme histochemical technique, for the demonstration of arylsulphatase activity, using 6-bromo-2-naphthylsulphate as a substrate, is biochemically tested by using partly purified lysosome and microsome preparations from fresh human placenta tissue. Microsomes from frozen placenta with an arylsulphatase deficiency and lysosomes from rat liver, are also investigated. For the biochemical test methods, 6-bromo-2-naphthylsulphate and p-nitrocatecholsulphate are used as substrates. Under similar reaction conditions, varying the pH of the incubation medium and adding inhibitors or activators, the histochemical and biochemical reactions are compared. The results of this study show that the enzyme histochemical technique--except for some limitations--is suitable for the demonstration of microsomal arylsulphatase in cryostat sections.", "contents": "Combined histochemical and biochemical investigation to the reliability of the demonstration of arylsulphatase activity in cryostat sections. The reliability of the enzyme histochemical technique, for the demonstration of arylsulphatase activity, using 6-bromo-2-naphthylsulphate as a substrate, is biochemically tested by using partly purified lysosome and microsome preparations from fresh human placenta tissue. Microsomes from frozen placenta with an arylsulphatase deficiency and lysosomes from rat liver, are also investigated. For the biochemical test methods, 6-bromo-2-naphthylsulphate and p-nitrocatecholsulphate are used as substrates. Under similar reaction conditions, varying the pH of the incubation medium and adding inhibitors or activators, the histochemical and biochemical reactions are compared. The results of this study show that the enzyme histochemical technique--except for some limitations--is suitable for the demonstration of microsomal arylsulphatase in cryostat sections."}
{"id": "PMID:489420", "title": "Parafollicular cells of rabbit thryoid store both calcitonin and somatostatin and resemble gut D cells ultrastructurally.", "content": "Both calcitonin and somatostatin have been detected immunohistochemically in rabbit parafollicular cells; only calcitonin has been found in the same cells of the dog, guinea-pig and man. Large amounts of a peptide radioimmunochemically identical with synthetic somatostatin have been detected in extracts of rabbit thyroid. The ultrastructural and staining features of rabbit parafollicular cells differ from those of parafollicular cells in other species, while resembling in part those of somatostatin D cells scattered in the rabbit stomach.", "contents": "Parafollicular cells of rabbit thryoid store both calcitonin and somatostatin and resemble gut D cells ultrastructurally. Both calcitonin and somatostatin have been detected immunohistochemically in rabbit parafollicular cells; only calcitonin has been found in the same cells of the dog, guinea-pig and man. Large amounts of a peptide radioimmunochemically identical with synthetic somatostatin have been detected in extracts of rabbit thyroid. The ultrastructural and staining features of rabbit parafollicular cells differ from those of parafollicular cells in other species, while resembling in part those of somatostatin D cells scattered in the rabbit stomach."}
{"id": "PMID:489421", "title": "Elemental analysis of solid microscopic samples in the flameless atomic absorption cuvette.", "content": "Solid microscopic samples are precisely located by means of a newly developed applicator under microscopic control in the center of a flameless graphite tube cuvette. The parts of the applicator that reach into the cuvette are made of quartz which can be cleaned by heating. The values for the K and Na content of samples such applied are highly reproducible. No contamination could be detected. Solid samples yield higher signals than liquid samples. A method of calibration is described which uses lyophilized pieces of egg albumin containing known amounts of K and Na as standards.", "contents": "Elemental analysis of solid microscopic samples in the flameless atomic absorption cuvette. Solid microscopic samples are precisely located by means of a newly developed applicator under microscopic control in the center of a flameless graphite tube cuvette. The parts of the applicator that reach into the cuvette are made of quartz which can be cleaned by heating. The values for the K and Na content of samples such applied are highly reproducible. No contamination could be detected. Solid samples yield higher signals than liquid samples. A method of calibration is described which uses lyophilized pieces of egg albumin containing known amounts of K and Na as standards."}
{"id": "PMID:489446", "title": "Iseikonic lens implantation in anisometropia.", "content": "The implantation of an iseikonic prepupillary intraocular lens in unilateral aphakia usually requires a slight (2 D) myopia of the implanted eye if the two eyes are isometropic. On the other hand, the implantation of an iseikonic implant in one eye of a anisometropic (asymmetric) eye pair can result in unexpectedly high changes in spectacle ametropia, due to differences in axial length or corneal power. The changes in ametropia after iseikonic lens implantation are discussed in relation to preoperative corneal and axial anisometropias. Alternative procedures to iseikonic implantation are described.", "contents": "Iseikonic lens implantation in anisometropia. The implantation of an iseikonic prepupillary intraocular lens in unilateral aphakia usually requires a slight (2 D) myopia of the implanted eye if the two eyes are isometropic. On the other hand, the implantation of an iseikonic implant in one eye of a anisometropic (asymmetric) eye pair can result in unexpectedly high changes in spectacle ametropia, due to differences in axial length or corneal power. The changes in ametropia after iseikonic lens implantation are discussed in relation to preoperative corneal and axial anisometropias. Alternative procedures to iseikonic implantation are described."}
{"id": "PMID:489448", "title": "300 primary anterior chamber lens implantations: gonioscopic findings and specular microscopy.", "content": "A total of 300 consecutive quadruped anterior chamber intraocular lenses were implanted. Gonioscopy was performed in all cases and prospective specular microscopy was performed in the last 97 cases. Iris tucking of at least one lens foot was noted in 28% of the cases. Tucking occurred less often (20% of the cases) when the lens length was 13 mm, increasing with lens lengths longer and shorter than 13 mm. Slight lens rotation was noted in 8% of the cases. Blood vessels (rarely appearing to be neovascular) were noted around an implant foot or in the anterior chamber angle near a lens foot in 30% of the cases. A statistically significant difference in endothelial cell loss was noted between the Choyce lens (18.6%) and the Tennant lens (12.4%).", "contents": "300 primary anterior chamber lens implantations: gonioscopic findings and specular microscopy. A total of 300 consecutive quadruped anterior chamber intraocular lenses were implanted. Gonioscopy was performed in all cases and prospective specular microscopy was performed in the last 97 cases. Iris tucking of at least one lens foot was noted in 28% of the cases. Tucking occurred less often (20% of the cases) when the lens length was 13 mm, increasing with lens lengths longer and shorter than 13 mm. Slight lens rotation was noted in 8% of the cases. Blood vessels (rarely appearing to be neovascular) were noted around an implant foot or in the anterior chamber angle near a lens foot in 30% of the cases. A statistically significant difference in endothelial cell loss was noted between the Choyce lens (18.6%) and the Tennant lens (12.4%)."}
{"id": "PMID:489449", "title": "Peripheral iris erosions associated with pediatric intraocular lens implants.", "content": "Eight children in whom 2-loop iridocapsular lenses or 2-loop iris suture lenses were implanted have exhibited peripheral iris erosion adjacent to the IOL haptic, through the iris stroma, and eventually through the iris pigment epithelium. The process appears to be very gradual and these patients have exhibited no signs of iris irritation of hyphema. No treatment has been instituted.", "contents": "Peripheral iris erosions associated with pediatric intraocular lens implants. Eight children in whom 2-loop iridocapsular lenses or 2-loop iris suture lenses were implanted have exhibited peripheral iris erosion adjacent to the IOL haptic, through the iris stroma, and eventually through the iris pigment epithelium. The process appears to be very gradual and these patients have exhibited no signs of iris irritation of hyphema. No treatment has been instituted."}
{"id": "PMID:489450", "title": "Clinical evaluation of the DBR A-scan unit.", "content": "One hundred and eight eyes of 106 patients were evaluated preoperatively with the DBR-300 A-scan ultrasonic instrument. Intraocular lens power was then calculated using the formula supplied with the instrument, and the accuracy of the instrument was evaluated. It was found that 61.1% of the eyes were within +/- 1.00 D of the calculated refractive error and 81.5% of the eyes were within +/- 1.50 D of the calculated refractive error when using a standard 3.5-mm anterior chamber depth. Various factors were then analyzed to determine which factors contributed to the inaccuracy of the instrument. No single factor could be found to account for the slight tendency toward myopia, although several were found to contribute. No single factor was found to contribute to large errors.", "contents": "Clinical evaluation of the DBR A-scan unit. One hundred and eight eyes of 106 patients were evaluated preoperatively with the DBR-300 A-scan ultrasonic instrument. Intraocular lens power was then calculated using the formula supplied with the instrument, and the accuracy of the instrument was evaluated. It was found that 61.1% of the eyes were within +/- 1.00 D of the calculated refractive error and 81.5% of the eyes were within +/- 1.50 D of the calculated refractive error when using a standard 3.5-mm anterior chamber depth. Various factors were then analyzed to determine which factors contributed to the inaccuracy of the instrument. No single factor could be found to account for the slight tendency toward myopia, although several were found to contribute. No single factor was found to contribute to large errors."}
{"id": "PMID:489462", "title": "Changes in membrane surface areas in mouse parietal cells in relation to high levels of acid secretion.", "content": "Levels of gastric acid secretion which may be maximal for the mouse were recorded following treatment with histamine and carbachol. A 30-fold increase over control levels was obtained in perfused animals, corresponding to a fourfold increase over highest levels recorded previously for stimulated mice. Stereological methods were used to estimate surface areas of membrane compartments of parietal cells in control and stimulated animals. Estimates of relative changes in membrane surface areas using a surface ratio method in this case substantiated changes detected by calculating surface densities. Main changes in membrane compartments of parietal cells from animals showing maximal acid secretion were a fourfold increase in free (luminal) surface, a 50% increase approximately in lateral and basal membrane, and a 90% reduction approximately in the tubulovesicular membrane compartment. Following withdrawal of secretagogues, acid secretion usually returned to control levels within 3 hours, but complete reconstitution of the tubulovesicular compartment was not seen within any survival period up to 5 hours. Reappearance of tubulovesicular elements first occurred shortly after the peak of a secretory response in focal cytoplasmic areas containing spherical and indented coated vesicles, and also numerous concentric membrane profiles not previously described in parietal cells. The way in which movement of membrane from the tubulovesicular compartment to the free surface occurs is not yet clear. However, reconstitution of the tubulovesicular compartment during a fall in acid secretion appears to involve movement of membrane from the free surface through coated vesicles, and their progression through indented forms and concentric membrane profiles to vesicles of the tubulovesicular compartment.", "contents": "Changes in membrane surface areas in mouse parietal cells in relation to high levels of acid secretion. Levels of gastric acid secretion which may be maximal for the mouse were recorded following treatment with histamine and carbachol. A 30-fold increase over control levels was obtained in perfused animals, corresponding to a fourfold increase over highest levels recorded previously for stimulated mice. Stereological methods were used to estimate surface areas of membrane compartments of parietal cells in control and stimulated animals. Estimates of relative changes in membrane surface areas using a surface ratio method in this case substantiated changes detected by calculating surface densities. Main changes in membrane compartments of parietal cells from animals showing maximal acid secretion were a fourfold increase in free (luminal) surface, a 50% increase approximately in lateral and basal membrane, and a 90% reduction approximately in the tubulovesicular membrane compartment. Following withdrawal of secretagogues, acid secretion usually returned to control levels within 3 hours, but complete reconstitution of the tubulovesicular compartment was not seen within any survival period up to 5 hours. Reappearance of tubulovesicular elements first occurred shortly after the peak of a secretory response in focal cytoplasmic areas containing spherical and indented coated vesicles, and also numerous concentric membrane profiles not previously described in parietal cells. The way in which movement of membrane from the tubulovesicular compartment to the free surface occurs is not yet clear. However, reconstitution of the tubulovesicular compartment during a fall in acid secretion appears to involve movement of membrane from the free surface through coated vesicles, and their progression through indented forms and concentric membrane profiles to vesicles of the tubulovesicular compartment."}
{"id": "PMID:489463", "title": "Two types of congenital hydrocephalus induced in rats by X-irradiation in utero: electron microscopic study on the telencephalic wall.", "content": "Stage-specific incidences of congenital hydrocephalus induced by X-irradiation of pregnant rats showed a bimodal distribution. At a dose level of 200 R, 100% hydrocephalic offspring were obtained by irradiation at embryonic days 11 and 14. When pregnant rats were subjected to 200 R X-irradiation at embryonic day 11, numerous ventricular cells of the telencephalic wall of the embryo became necrotic during the first 2 hours post-irradiation, but the paraventricular cell-to-cell interconnexions made up of zonulae adhaerentes were less affected. Mitosis took place in the surviving paraventricular surface cells throughout subsequent development. The full-term fetus exhibited little change in the cytoarchitectural arrangement of neural cells and neuropils, although it was only about half the thickness of the untreated control. After 200 R X-irradiation at embryonic day 14, most of the ventricular cells became necrotic within 6 hours. The paraventricular cell-to-cell interconnexions were completely destroyed, and never repaired in subsequent development. Mitosis took place either freely in cell clusters, or in rosettes which formed randomly in the telencephalic wall between 48 and 72 hours post-irradiation. The resulting telencephalic wall of the full-term fetus was also about half the thickness of the control. In the outer part of the tissue, the cortical plate made up of differentiating neuroblasts was hypoplastic, but the inner half was filled with numerous heterotopic masses of pleomorphic cells and bundles of primitive axons. The ependymal layer at the paraventricular surface was never formed. Whether the paraventricular zonulae adhaerentes were destroyed or not by X-irradiation was considered to be an important factor in the determination of the subsequent cytoarchitectural organization of the telencephalic wall.", "contents": "Two types of congenital hydrocephalus induced in rats by X-irradiation in utero: electron microscopic study on the telencephalic wall. Stage-specific incidences of congenital hydrocephalus induced by X-irradiation of pregnant rats showed a bimodal distribution. At a dose level of 200 R, 100% hydrocephalic offspring were obtained by irradiation at embryonic days 11 and 14. When pregnant rats were subjected to 200 R X-irradiation at embryonic day 11, numerous ventricular cells of the telencephalic wall of the embryo became necrotic during the first 2 hours post-irradiation, but the paraventricular cell-to-cell interconnexions made up of zonulae adhaerentes were less affected. Mitosis took place in the surviving paraventricular surface cells throughout subsequent development. The full-term fetus exhibited little change in the cytoarchitectural arrangement of neural cells and neuropils, although it was only about half the thickness of the untreated control. After 200 R X-irradiation at embryonic day 14, most of the ventricular cells became necrotic within 6 hours. The paraventricular cell-to-cell interconnexions were completely destroyed, and never repaired in subsequent development. Mitosis took place either freely in cell clusters, or in rosettes which formed randomly in the telencephalic wall between 48 and 72 hours post-irradiation. The resulting telencephalic wall of the full-term fetus was also about half the thickness of the control. In the outer part of the tissue, the cortical plate made up of differentiating neuroblasts was hypoplastic, but the inner half was filled with numerous heterotopic masses of pleomorphic cells and bundles of primitive axons. The ependymal layer at the paraventricular surface was never formed. Whether the paraventricular zonulae adhaerentes were destroyed or not by X-irradiation was considered to be an important factor in the determination of the subsequent cytoarchitectural organization of the telencephalic wall."}
{"id": "PMID:489465", "title": "Corticosteroid-induced mandibular growth retardation and palatal malformation in the ICR mouse fetus.", "content": "Pregnant ICR mice were treated with triamcinolone hexacetonide at various stages of gestation. The mandibular ramus and its condylar cartilage were studied histologically in both viable and non-viable offspring. In addition, measurements were made of the overall height of the posterior vertical dimension of the mandible and of condylar height and width. Significant changes were noted in these parameters. Concomitantly, marked changes were observed in the various zones of the condylar cartilage. A very high incidence of cleft palate was noted in newborn and stillborn mice previously treated with triamcinolone. A possible correlation between mandibular growth retardation and palatal clefting is discussed.", "contents": "Corticosteroid-induced mandibular growth retardation and palatal malformation in the ICR mouse fetus. Pregnant ICR mice were treated with triamcinolone hexacetonide at various stages of gestation. The mandibular ramus and its condylar cartilage were studied histologically in both viable and non-viable offspring. In addition, measurements were made of the overall height of the posterior vertical dimension of the mandible and of condylar height and width. Significant changes were noted in these parameters. Concomitantly, marked changes were observed in the various zones of the condylar cartilage. A very high incidence of cleft palate was noted in newborn and stillborn mice previously treated with triamcinolone. A possible correlation between mandibular growth retardation and palatal clefting is discussed."}
{"id": "PMID:489466", "title": "A radiographic study of the human fetal spine. 3. Longitudinal growth.", "content": "Regression equations are presented which describe the growth in length of the various regions of the vertebral column in the human fetus. From 8 weeks on the thoracic is always the longest region and the sacral the shortest, while the lumbar region is longer than the cervical. From the regression equations predictions of fetal vertebral length can be made from fetal age: this should be useful in obstetric practice when diagnostic ultrasound techniques are being employed for the diagnosis of growth disorders and skeletal abnormalities. A different developmental pattern emerges when average 'vertebral units' for each region are compared. The lumbar vertebrae are always the largest with the thoracic, cervical and sacral vertebrae being progressively smaller.", "contents": "A radiographic study of the human fetal spine. 3. Longitudinal growth. Regression equations are presented which describe the growth in length of the various regions of the vertebral column in the human fetus. From 8 weeks on the thoracic is always the longest region and the sacral the shortest, while the lumbar region is longer than the cervical. From the regression equations predictions of fetal vertebral length can be made from fetal age: this should be useful in obstetric practice when diagnostic ultrasound techniques are being employed for the diagnosis of growth disorders and skeletal abnormalities. A different developmental pattern emerges when average 'vertebral units' for each region are compared. The lumbar vertebrae are always the largest with the thoracic, cervical and sacral vertebrae being progressively smaller."}
{"id": "PMID:489467", "title": "Reactions of transplanted neurocentral synchondroses to different conditions of mechanical stress. A methodological study on the rat.", "content": "In order to elucidate the reactions of neurocentral synchondroses to different forces, the first cervical vertebra of 10 or 25 days old rats was transplanted into sex-matched litter mates. Some vertebrae were transplanted as a whole, in some only the ventral part with its synchondroses was transplanted and, in others the lumen was furnished with an expanding sponge or a spring. The transplantation was done subcutaneously and, in the case of the fragments, intracerebrally as well. The synchondroses of the vertebrae transplanted at 10 days did not differ very much from those of the host 5, 10 or 15 days after the operation, whereas in the vertebrae transplanted at 25 days the synchondroses underwent synostosis earlier than in situ. The synchondroses of the transplanted fragments, and especially of those placed intracerebrally, remained open longer than those in the whole vertebral transplants; the sponge and and the spring also delayed closure. In the synchondroses transplanted at 25 days there was a strong reduction in alcian blue staining, whereas in the spring loaded synchondroses the stainability persisted longer, maybe as an adaptation to the tensile force. It seems that the inherent potential of the neurocentral synchondroses to obliterate at a certain time can be altered by changing the biomechanical conditions.", "contents": "Reactions of transplanted neurocentral synchondroses to different conditions of mechanical stress. A methodological study on the rat. In order to elucidate the reactions of neurocentral synchondroses to different forces, the first cervical vertebra of 10 or 25 days old rats was transplanted into sex-matched litter mates. Some vertebrae were transplanted as a whole, in some only the ventral part with its synchondroses was transplanted and, in others the lumen was furnished with an expanding sponge or a spring. The transplantation was done subcutaneously and, in the case of the fragments, intracerebrally as well. The synchondroses of the vertebrae transplanted at 10 days did not differ very much from those of the host 5, 10 or 15 days after the operation, whereas in the vertebrae transplanted at 25 days the synchondroses underwent synostosis earlier than in situ. The synchondroses of the transplanted fragments, and especially of those placed intracerebrally, remained open longer than those in the whole vertebral transplants; the sponge and and the spring also delayed closure. In the synchondroses transplanted at 25 days there was a strong reduction in alcian blue staining, whereas in the spring loaded synchondroses the stainability persisted longer, maybe as an adaptation to the tensile force. It seems that the inherent potential of the neurocentral synchondroses to obliterate at a certain time can be altered by changing the biomechanical conditions."}
{"id": "PMID:489468", "title": "Comparative kinematic analysis of bipedal and quadrupedal locomotion: a cyclographic technique.", "content": "This paper shows the convention for, and applicability of, a new computer-aided cyclographic technique for kinematic analysis of locomotion in quadrupeds. Comparison is made of the walking, trotting and galloping patterns of a reference quadruped, Canis familiaris. The method is effective where an investigator required an accurate evaluation of limb movement in the cranio-caudal plane.", "contents": "Comparative kinematic analysis of bipedal and quadrupedal locomotion: a cyclographic technique. This paper shows the convention for, and applicability of, a new computer-aided cyclographic technique for kinematic analysis of locomotion in quadrupeds. Comparison is made of the walking, trotting and galloping patterns of a reference quadruped, Canis familiaris. The method is effective where an investigator required an accurate evaluation of limb movement in the cranio-caudal plane."}
{"id": "PMID:489469", "title": "Total fibre numbers in cross sections of the semitendinosus in athletic and non-athletic horses and dogs.", "content": "The numbers of fibres in the complete cross section of the semitendinosus were estimated in 58 equines and 59 canines of differing types. Animals selected for swiftness--thoroughbred horses and greyhounds--have a greater number of fibres in a cross section of their semitendinosus than other members of their species, whether as neonates or as adults. The numbers of fibres in the cross section increases during growth in both types of animal in each species.", "contents": "Total fibre numbers in cross sections of the semitendinosus in athletic and non-athletic horses and dogs. The numbers of fibres in the complete cross section of the semitendinosus were estimated in 58 equines and 59 canines of differing types. Animals selected for swiftness--thoroughbred horses and greyhounds--have a greater number of fibres in a cross section of their semitendinosus than other members of their species, whether as neonates or as adults. The numbers of fibres in the cross section increases during growth in both types of animal in each species."}
{"id": "PMID:489470", "title": "Anatomical closure of the ductus arteriosus: a study in 35 specimens.", "content": "The process of anatomical ductal obliteration appears to be gradual and to involve proliferation of intima and media (mainly the latter) producing mounds, mucoid-filled spaces and fragmentation of the internal elastic membrane. Dissociation of the inner media may also be involved. The persistent ductus can be recognized histologically, and its clinical significance is discussed.", "contents": "Anatomical closure of the ductus arteriosus: a study in 35 specimens. The process of anatomical ductal obliteration appears to be gradual and to involve proliferation of intima and media (mainly the latter) producing mounds, mucoid-filled spaces and fragmentation of the internal elastic membrane. Dissociation of the inner media may also be involved. The persistent ductus can be recognized histologically, and its clinical significance is discussed."}
{"id": "PMID:489471", "title": "Lymphoid response to pregnancy and pseudopregnancy in the rat.", "content": "Differences in weight, while cell density and differential white cell count of the thymus, spleen, iliac and popliteal lymph nodes of virgin, 10 days outbred pregnant, 10 days inbred pregnant, 10 days pseudopregnant, and 40 days outbred post-coitum rat were examined. Compared with virgin animals, significant weight gain occurred only in the thymus, spleen and iliac lymph nodes of the outbred group. Significant weight loss from the thymus and iliac nodes, and significant reductions in white cell density of the spleen and popliteal nodes occurred in delivered animals when compared with the virgin group. During pseudopregnancy significant weight loss occurred from the thymus, spleen and popliteal lymph nodes compared with the inbred group, and from the iliac lymph nodes compared with the virgin group. No significant differences were observed between the proportions of proliferating cells in the lymphocyte populations of the thymus, spleen, iliac and popliteal lymph nodes of the virgin group. In the outbred group the proliferating populations of all four tissues rose significantly above virgin levels, while in the inbred group only the iliac lymph nodes showed any significant rise above the virgin value. These findings are discussed in relation to the maternal immunological response to pregnancy.", "contents": "Lymphoid response to pregnancy and pseudopregnancy in the rat. Differences in weight, while cell density and differential white cell count of the thymus, spleen, iliac and popliteal lymph nodes of virgin, 10 days outbred pregnant, 10 days inbred pregnant, 10 days pseudopregnant, and 40 days outbred post-coitum rat were examined. Compared with virgin animals, significant weight gain occurred only in the thymus, spleen and iliac lymph nodes of the outbred group. Significant weight loss from the thymus and iliac nodes, and significant reductions in white cell density of the spleen and popliteal nodes occurred in delivered animals when compared with the virgin group. During pseudopregnancy significant weight loss occurred from the thymus, spleen and popliteal lymph nodes compared with the inbred group, and from the iliac lymph nodes compared with the virgin group. No significant differences were observed between the proportions of proliferating cells in the lymphocyte populations of the thymus, spleen, iliac and popliteal lymph nodes of the virgin group. In the outbred group the proliferating populations of all four tissues rose significantly above virgin levels, while in the inbred group only the iliac lymph nodes showed any significant rise above the virgin value. These findings are discussed in relation to the maternal immunological response to pregnancy."}
{"id": "PMID:489472", "title": "Transformation of monocytes into amoeboid microglia in the corpus callosum of postnatal rats, as shown by labelling monocytes by carbon particles.", "content": "Two successive intravenous doses of carbon suspension were given at 24 hourly intervals into six days old rats. These animals were killed at intervals ranging from 1 to 9 days after the second injection. The corpus callosum and neighbouring structures were examined for cells containing ingested colloidal carbon particles in their cytoplasm. Twenty four hours after the second injection, a variable number of carbon-labelled monocytes were adherent to the luminal wall of blood vessels in the corpus callosum. Numerous carbon-labelled cells appeared to have left the lumen and entered the brain tissue surrounding the vessels. These perivascular carbon-labelled monocytes in the neuropil displayed a large pale nucleus with fine chromatin granules. The phagocytic amoeboid microglia in the corpus callosum were unlabelled at first, although a few cells of a similar nature in the cavum septi pellucidi did show carbon particles in their cytoplasm. Four or five days after the second carbon injection perivascular carbon-labelled monocytes were rare, but carbon particles were now present in the amoeboid microglia. At 8 days amoeboid microglia were virtually absent from the corpus callosum but carbon particles now appeared in cells which closely resembled microglia (flattened nucleus, coarse chromatin, scanty cytoplasm at one pole). The sequential appearance of carbon particles in monocytes, amoeboid microglia, and microglia, suggests that monocytes transform into microglia by way of an amoeboid microglial stage.", "contents": "Transformation of monocytes into amoeboid microglia in the corpus callosum of postnatal rats, as shown by labelling monocytes by carbon particles. Two successive intravenous doses of carbon suspension were given at 24 hourly intervals into six days old rats. These animals were killed at intervals ranging from 1 to 9 days after the second injection. The corpus callosum and neighbouring structures were examined for cells containing ingested colloidal carbon particles in their cytoplasm. Twenty four hours after the second injection, a variable number of carbon-labelled monocytes were adherent to the luminal wall of blood vessels in the corpus callosum. Numerous carbon-labelled cells appeared to have left the lumen and entered the brain tissue surrounding the vessels. These perivascular carbon-labelled monocytes in the neuropil displayed a large pale nucleus with fine chromatin granules. The phagocytic amoeboid microglia in the corpus callosum were unlabelled at first, although a few cells of a similar nature in the cavum septi pellucidi did show carbon particles in their cytoplasm. Four or five days after the second carbon injection perivascular carbon-labelled monocytes were rare, but carbon particles were now present in the amoeboid microglia. At 8 days amoeboid microglia were virtually absent from the corpus callosum but carbon particles now appeared in cells which closely resembled microglia (flattened nucleus, coarse chromatin, scanty cytoplasm at one pole). The sequential appearance of carbon particles in monocytes, amoeboid microglia, and microglia, suggests that monocytes transform into microglia by way of an amoeboid microglial stage."}
{"id": "PMID:489473", "title": "Autonomic innervation of the muscles in the wall of the bladder and proximal urethra of male rats.", "content": "The muscular coat of the body of the rat bladder is innervated almost exclusively by cholinergic endings:adrenergic endings are rare. In the inner longitudinal muscle layer of the proximal urethra, 53% of 310 autonomic nerve endings observed in close relation to the smooth muscle cells were adrenergic and the remaining 47% cholinergic. The middle circular muscle layer of the proximal urethra was innervated predominantly by adrenergic endings: in this layer 86% of the total of 335 endings examined wre regarded as adrenergic. A similar predominantly adrenergic innervation was noted in the outer longitudinal layer of the proximal urethra. A number of striated muscle fibres arose from the outermost striated muscle layer of the proximal urethra and intruded deeply into the outer and middle smooth muscle layers. These intruding striated muscle fibres also received direct autonomic (mostly adrenergic) innervation. The significance of these findings in relation to the physiology of the lower urinary tracts is discussed.", "contents": "Autonomic innervation of the muscles in the wall of the bladder and proximal urethra of male rats. The muscular coat of the body of the rat bladder is innervated almost exclusively by cholinergic endings:adrenergic endings are rare. In the inner longitudinal muscle layer of the proximal urethra, 53% of 310 autonomic nerve endings observed in close relation to the smooth muscle cells were adrenergic and the remaining 47% cholinergic. The middle circular muscle layer of the proximal urethra was innervated predominantly by adrenergic endings: in this layer 86% of the total of 335 endings examined wre regarded as adrenergic. A similar predominantly adrenergic innervation was noted in the outer longitudinal layer of the proximal urethra. A number of striated muscle fibres arose from the outermost striated muscle layer of the proximal urethra and intruded deeply into the outer and middle smooth muscle layers. These intruding striated muscle fibres also received direct autonomic (mostly adrenergic) innervation. The significance of these findings in relation to the physiology of the lower urinary tracts is discussed."}
{"id": "PMID:489476", "title": "Withdrawal of chronic chlorpromazine medication: an experimental analysis.", "content": "Approximately 50% of all institutionalized, mentally retarded adults receive psychotropic medication to control inappropriate behavior. In this study, behaviors exhibited by five retarded adults were formally observed while they were on and off medication. Each subject had been receiving chlorpromazine for six or more years prior to the start of the study. The drug was withdrawn and readministered using a double-blind B-A-B (drug placebo-drug) design. Effects were highly individualized. Some desirable behavior emerged when chlorpromazine was discontinued.", "contents": "Withdrawal of chronic chlorpromazine medication: an experimental analysis. Approximately 50% of all institutionalized, mentally retarded adults receive psychotropic medication to control inappropriate behavior. In this study, behaviors exhibited by five retarded adults were formally observed while they were on and off medication. Each subject had been receiving chlorpromazine for six or more years prior to the start of the study. The drug was withdrawn and readministered using a double-blind B-A-B (drug placebo-drug) design. Effects were highly individualized. Some desirable behavior emerged when chlorpromazine was discontinued."}
{"id": "PMID:489477", "title": "Suppression of self-stimulation: three alternative strategies.", "content": "Four boys with autistic-like behavior were treated for self-stimulatory behavior with three different treatment procedures--time out, differential reinforcement of other behavior (DRO), and overcorrection. All four boys showed a rapid response to the overcorrection procedure. Three boys demonstrated some evidence of decrement in responding with time-out. During the DRO procedure, one showed a modest decrease, two showed no change, but one exhibited a consistent increase in responding under this condition. A multiple baseline applied to one of the subjects failed to reveal any generalization of suppression from one setting to another. A strong but not perfect relationship was found between a frequency and a duration measure of self-stimulation. There was some evidence of negative side effects for one boy during overcorrection and for another during time-out. None of these negative side effects was enduring. There was also some indirect evidence that overcorrection facilitated appropriate play.", "contents": "Suppression of self-stimulation: three alternative strategies. Four boys with autistic-like behavior were treated for self-stimulatory behavior with three different treatment procedures--time out, differential reinforcement of other behavior (DRO), and overcorrection. All four boys showed a rapid response to the overcorrection procedure. Three boys demonstrated some evidence of decrement in responding with time-out. During the DRO procedure, one showed a modest decrease, two showed no change, but one exhibited a consistent increase in responding under this condition. A multiple baseline applied to one of the subjects failed to reveal any generalization of suppression from one setting to another. A strong but not perfect relationship was found between a frequency and a duration measure of self-stimulation. There was some evidence of negative side effects for one boy during overcorrection and for another during time-out. None of these negative side effects was enduring. There was also some indirect evidence that overcorrection facilitated appropriate play."}
{"id": "PMID:489478", "title": "Alternating treatments design: one strategy for comparing the effects of two treatments in a single subject.", "content": "A little used and often confused design, capable of comparing two treatments within a single subject, has been termed, variously, a multielement baseline design, a multiple schedule design, and a randomization design. The background of these terms is reviewed, and a new, more descriptive term, Alternating Treatments Design, is proposed. Critical differences between this design and a Simultaneous Treatment Design are outlined, and experimental questions answerable by each design are noted. Potential problems with multiple treatment interference in this procedure are divided into sequential confounding, carryover effects, and alternation effects and the importance of these issues vis-a-vis other single-case experimental designs is considered. Methods of minimizing multiple treatment interference as well as methods of studying these effects are outlined. Finally, appropriate uses of Alternating Treatments Designs are described and discussed in the context of recent examples.", "contents": "Alternating treatments design: one strategy for comparing the effects of two treatments in a single subject. A little used and often confused design, capable of comparing two treatments within a single subject, has been termed, variously, a multielement baseline design, a multiple schedule design, and a randomization design. The background of these terms is reviewed, and a new, more descriptive term, Alternating Treatments Design, is proposed. Critical differences between this design and a Simultaneous Treatment Design are outlined, and experimental questions answerable by each design are noted. Potential problems with multiple treatment interference in this procedure are divided into sequential confounding, carryover effects, and alternation effects and the importance of these issues vis-a-vis other single-case experimental designs is considered. Methods of minimizing multiple treatment interference as well as methods of studying these effects are outlined. Finally, appropriate uses of Alternating Treatments Designs are described and discussed in the context of recent examples."}
{"id": "PMID:489479", "title": "Effects of self-instructional training on second- and third-grade hyperactive children: a failure to replicate.", "content": "Bornstein and Quevillon (1976) demonstrated generalization from a 2-hour self-instructional training session to on-task behavior in the classroom with 4-year-old overactive children. In an attempt to replicate this work with older children, eight 7- and 8-year-old hyperactive children were assigned to either a self-instructional training group or an attention-practice control group. On-task behavior in the classroom and performance measures in reading and arithmetic were assessed. The level of difficulty of these tasks was varied. The results of Bornstein and Quevillon's (1976) study were not replicated, although the subsequent introduction of a token program significantly increased on-task behavior.", "contents": "Effects of self-instructional training on second- and third-grade hyperactive children: a failure to replicate. Bornstein and Quevillon (1976) demonstrated generalization from a 2-hour self-instructional training session to on-task behavior in the classroom with 4-year-old overactive children. In an attempt to replicate this work with older children, eight 7- and 8-year-old hyperactive children were assigned to either a self-instructional training group or an attention-practice control group. On-task behavior in the classroom and performance measures in reading and arithmetic were assessed. The level of difficulty of these tasks was varied. The results of Bornstein and Quevillon's (1976) study were not replicated, although the subsequent introduction of a token program significantly increased on-task behavior."}
{"id": "PMID:489480", "title": "Sensory extinction and sensory reinforcement principles for programming multiple adaptive behavior change.", "content": "The role of sensory reinforcement was examined in programming multiple treatment gains in self-stimulation and spontaneous play for developmentally disabled children. Two phases were planned. First, we attempted to identify reinforcers maintaining self-stimulation. Sensory Extinction procedures were implemented in which auditory, proprioceptive, or visual sensory consequences of self-stimulatory behavior were systematically removed and reintroduced in a reversal design. When self-stimulation was decreased or eliminated as a result of removing one of these sensory consequences, the functional sensory consequence was designated as a child's preferred sensory reinforcer. In Phase 2, we assessed whether children would play selectively with toys producing the preferred kind of sensory stimulation. The results showed the following. (1) Self-stimulatory behavior was found to be maintained by sensory reinforcement. When the sensory reinforcer was removed, self-stimulation extinguished. (2) The sensory reinforcers identified for self-stimulatory behavior also served as reinforcers for new, appropriate toy play. (3) The multiple treatment gains observed appeared to be relatively durable in the absence of external reinforcers for play or restraints on self-stimulation. These results illustrate one instance in which multiple behavior change may be programmed in a predictable, lawful fashion by using \"natural communities of sensory reinforcement.\"", "contents": "Sensory extinction and sensory reinforcement principles for programming multiple adaptive behavior change. The role of sensory reinforcement was examined in programming multiple treatment gains in self-stimulation and spontaneous play for developmentally disabled children. Two phases were planned. First, we attempted to identify reinforcers maintaining self-stimulation. Sensory Extinction procedures were implemented in which auditory, proprioceptive, or visual sensory consequences of self-stimulatory behavior were systematically removed and reintroduced in a reversal design. When self-stimulation was decreased or eliminated as a result of removing one of these sensory consequences, the functional sensory consequence was designated as a child's preferred sensory reinforcer. In Phase 2, we assessed whether children would play selectively with toys producing the preferred kind of sensory stimulation. The results showed the following. (1) Self-stimulatory behavior was found to be maintained by sensory reinforcement. When the sensory reinforcer was removed, self-stimulation extinguished. (2) The sensory reinforcers identified for self-stimulatory behavior also served as reinforcers for new, appropriate toy play. (3) The multiple treatment gains observed appeared to be relatively durable in the absence of external reinforcers for play or restraints on self-stimulation. These results illustrate one instance in which multiple behavior change may be programmed in a predictable, lawful fashion by using \"natural communities of sensory reinforcement.\""}
{"id": "PMID:489481", "title": "Multiple-baseline analysis of a token economy for psychiatric inpatients.", "content": "Twelve behaviors selected for reinforcement among 16 chronic psychiatric inpatients were divided into four classes: (a) personal hygiene, (b) personal management, (c) ward work, and (d) social skills. A token economy program was introduced for each class in a sequential, cumulative, multiple-baseline format. Corrections were included for methodological deficiencies frequently enountered in past studies. Treatment variables were systematically monitored, and target behavior rates, levels of global individual functioning, general ward behavior, and off-ward behavior were assessed during baseline, implementation, and probe periods. Results indicated abrupt and substantial increases in performance of most target behaviors, significant improvements in global individual functioning (p less than .025), positive changes in general ward behavior, and increases in social interaction during off-ward activities. The findings provide strong evidence for the efficacy of a token economy and indicate that the multiple-baseline design can be a useful method for evaluating token economy programs.", "contents": "Multiple-baseline analysis of a token economy for psychiatric inpatients. Twelve behaviors selected for reinforcement among 16 chronic psychiatric inpatients were divided into four classes: (a) personal hygiene, (b) personal management, (c) ward work, and (d) social skills. A token economy program was introduced for each class in a sequential, cumulative, multiple-baseline format. Corrections were included for methodological deficiencies frequently enountered in past studies. Treatment variables were systematically monitored, and target behavior rates, levels of global individual functioning, general ward behavior, and off-ward behavior were assessed during baseline, implementation, and probe periods. Results indicated abrupt and substantial increases in performance of most target behaviors, significant improvements in global individual functioning (p less than .025), positive changes in general ward behavior, and increases in social interaction during off-ward activities. The findings provide strong evidence for the efficacy of a token economy and indicate that the multiple-baseline design can be a useful method for evaluating token economy programs."}
{"id": "PMID:489482", "title": "Generalization by autistic-type children of verbal responses across settings.", "content": "Generalization of verbal behavior by autistic-type children across physically different settings was assessed. Four boys learned responses to common questions in two settings at school and were probed to determine transfer of learning to home. Three of the children demonstrated little generalization to home when trained in a cubicle. Greater generalization was indicated when they received training at varied locations. The fourth child generalized most responses to his home regardless of training setting. Simple manipulations of the school environment to more closely simulate home conditions may facilitate transfer of training to the natural environment.", "contents": "Generalization by autistic-type children of verbal responses across settings. Generalization of verbal behavior by autistic-type children across physically different settings was assessed. Four boys learned responses to common questions in two settings at school and were probed to determine transfer of learning to home. Three of the children demonstrated little generalization to home when trained in a cubicle. Greater generalization was indicated when they received training at varied locations. The fourth child generalized most responses to his home regardless of training setting. Simple manipulations of the school environment to more closely simulate home conditions may facilitate transfer of training to the natural environment."}
{"id": "PMID:489485", "title": "Enuresis control through fading, escape, and avoidance training.", "content": "A twin-signal device that provides both escape and avoidance conditioning in ensuresis control is described involving a procedure documented by two case studies. In addition, a technique of fading as an adjunct to the process is utilized with one subject. The results indicate that a combination of operant and respondent conditioning involving escape and avoidance training may be an improvement over the more traditional conditioning procedure.", "contents": "Enuresis control through fading, escape, and avoidance training. A twin-signal device that provides both escape and avoidance conditioning in ensuresis control is described involving a procedure documented by two case studies. In addition, a technique of fading as an adjunct to the process is utilized with one subject. The results indicate that a combination of operant and respondent conditioning involving escape and avoidance training may be an improvement over the more traditional conditioning procedure."}
{"id": "PMID:489508", "title": "Medication compliance in chronic asthmatic patients.", "content": "In an attempt to assess patient and physician behavior patterns which interfere with effective medical treatment, 100 chronic adult and adolescent asthmatics were asked about the frequency and the nature of their medication non-compliance. The nature of the patient's medication abuse was also related to underlying personality characteristics, as measured by the MMPI Panic-Fear scale. Finally eleven physicians were asked about the frequency with which they asked their patients about over abuse and under abuse of prescribed medications. The results are impressive, showing that 54.0% of the patients reported some type of medication abuse, and that the nature of the abuse may be predicted from the patient's MMPI Panic-Fear score. Perhaps the most important finding was that while over 50% of the patients reported medication non-compliance (under abuse, over abuse, or cyclical abuse), only 27.3% of the physicians said that they routinely inquired about medication abuse. Such a discrepancy suggests one area in which patient and physician characteristics may have a bearing on the effective delivery of medical treatment. The findings underscore the need for physicians to include in their diagnostic procedure a routine inquiry into the patient's patterns of medication non-compliance.", "contents": "Medication compliance in chronic asthmatic patients. In an attempt to assess patient and physician behavior patterns which interfere with effective medical treatment, 100 chronic adult and adolescent asthmatics were asked about the frequency and the nature of their medication non-compliance. The nature of the patient's medication abuse was also related to underlying personality characteristics, as measured by the MMPI Panic-Fear scale. Finally eleven physicians were asked about the frequency with which they asked their patients about over abuse and under abuse of prescribed medications. The results are impressive, showing that 54.0% of the patients reported some type of medication abuse, and that the nature of the abuse may be predicted from the patient's MMPI Panic-Fear score. Perhaps the most important finding was that while over 50% of the patients reported medication non-compliance (under abuse, over abuse, or cyclical abuse), only 27.3% of the physicians said that they routinely inquired about medication abuse. Such a discrepancy suggests one area in which patient and physician characteristics may have a bearing on the effective delivery of medical treatment. The findings underscore the need for physicians to include in their diagnostic procedure a routine inquiry into the patient's patterns of medication non-compliance."}
{"id": "PMID:489509", "title": "Allergenic factors and symptomatology of respiratory allergy patients.", "content": "An attempt has been made in the present study to find out the relationship between the symptomatology of nasobronchial allergy patients of Delhi with markedly positive skin reactions and prevalence of total pollen concentration in the atmosphere, concentration of major individual pollen types, total fungal spores and house dust mites together with climatic factors such as mean monthly temperature and relative humidity. A statistically significant correlation was not found between symptomatology of the patients of Delhi and mean monthly relatively humidity, atmospheric concentration of pollen, fungal spores and house dust mites. A significant negative correlation was observed between symptomatology of patients and mean monthly temperature. Except for the pollen of prospis which showed significant positive correlation with the symptomatology of Delhi patients, a significant positive correlation was absent with respect to other individual pollen types.", "contents": "Allergenic factors and symptomatology of respiratory allergy patients. An attempt has been made in the present study to find out the relationship between the symptomatology of nasobronchial allergy patients of Delhi with markedly positive skin reactions and prevalence of total pollen concentration in the atmosphere, concentration of major individual pollen types, total fungal spores and house dust mites together with climatic factors such as mean monthly temperature and relative humidity. A statistically significant correlation was not found between symptomatology of the patients of Delhi and mean monthly relatively humidity, atmospheric concentration of pollen, fungal spores and house dust mites. A significant negative correlation was observed between symptomatology of patients and mean monthly temperature. Except for the pollen of prospis which showed significant positive correlation with the symptomatology of Delhi patients, a significant positive correlation was absent with respect to other individual pollen types."}
{"id": "PMID:489510", "title": "A comparative dermatoglyphic study of autistic, retarded, and normal children.", "content": "Dermatoglyphic comparisons were made among 32 autistic children aged from 4-10 to 18-11; sex-, age-, and IQ-matched retarded children; and sex- and age-matched normal children. Significant differences were found between the autistic and normal children for distribution of dermal patterns and ridge line disruption, but no significant differences were found for the total mean ridge counts or mean ridge count rankings. Apart from the right hand of the autistic children, there were no unusual scores for digital dispersion ratios. Autistic and retarded children differed only in their distribution of dermal patterns, with the autistic children apparently intermediate between retarded and normal groups. These results indicate that conclusions of unique congenital disturbance in the etiology of autism inferred from different dermatoglyphics may be premature, and that dermatoglyphics may be ineffective in delineating autistic children from other atypical populations.", "contents": "A comparative dermatoglyphic study of autistic, retarded, and normal children. Dermatoglyphic comparisons were made among 32 autistic children aged from 4-10 to 18-11; sex-, age-, and IQ-matched retarded children; and sex- and age-matched normal children. Significant differences were found between the autistic and normal children for distribution of dermal patterns and ridge line disruption, but no significant differences were found for the total mean ridge counts or mean ridge count rankings. Apart from the right hand of the autistic children, there were no unusual scores for digital dispersion ratios. Autistic and retarded children differed only in their distribution of dermal patterns, with the autistic children apparently intermediate between retarded and normal groups. These results indicate that conclusions of unique congenital disturbance in the etiology of autism inferred from different dermatoglyphics may be premature, and that dermatoglyphics may be ineffective in delineating autistic children from other atypical populations."}
{"id": "PMID:489511", "title": "Personal pronouns and the autistic child.", "content": "The long-recognized difficulties of the speaking autistic child with the use and nonuse of personal pronouns [\"reversals\" and \"avoidance\"] have been generally attributed either to the nondifferentiation of the self or to the frequently coexisting symptom of echolalia. These explanations are reconsidered in this eclectic analysis from the perspective of current theory and research in development of self and of language. Emphasis is on studies of normal development of personal pronouns and the roles played in that process by listening, echoic memory, mitigated echolalia [recoding], and person deixis. It is concluded that multiple developmental obstacles of a social, cognitive, and grammatical nature underlie the more obvious symptoms and militate against the child's resolution of labels and their referents. Treatment alternatives that de-emphasize the primacy of I are offered.", "contents": "Personal pronouns and the autistic child. The long-recognized difficulties of the speaking autistic child with the use and nonuse of personal pronouns [\"reversals\" and \"avoidance\"] have been generally attributed either to the nondifferentiation of the self or to the frequently coexisting symptom of echolalia. These explanations are reconsidered in this eclectic analysis from the perspective of current theory and research in development of self and of language. Emphasis is on studies of normal development of personal pronouns and the roles played in that process by listening, echoic memory, mitigated echolalia [recoding], and person deixis. It is concluded that multiple developmental obstacles of a social, cognitive, and grammatical nature underlie the more obvious symptoms and militate against the child's resolution of labels and their referents. Treatment alternatives that de-emphasize the primacy of I are offered."}
{"id": "PMID:489512", "title": "Perception of contingency and mental retardation.", "content": "It is argued that the general learning difficulties exhibited by mentally retarded persons are similar in many respects to the learning difficulties of nonretarded persons who are in a state of learned helplessness (Seligman, 1975) or who are external in locus of control orientation. It is suggested that studies of locus of control and learned helplessness as theoretical constructs both directly concern the perceiving of relationships between behavior and outcomes, and that a systematic investigation of perception of contingency might increase our understanding of some of the learning difficulties of mentally retarded persons. An overview is given of the results of the few studies that have addressed perception of contingency and mental retardation, and suggestions for future research are offered.", "contents": "Perception of contingency and mental retardation. It is argued that the general learning difficulties exhibited by mentally retarded persons are similar in many respects to the learning difficulties of nonretarded persons who are in a state of learned helplessness (Seligman, 1975) or who are external in locus of control orientation. It is suggested that studies of locus of control and learned helplessness as theoretical constructs both directly concern the perceiving of relationships between behavior and outcomes, and that a systematic investigation of perception of contingency might increase our understanding of some of the learning difficulties of mentally retarded persons. An overview is given of the results of the few studies that have addressed perception of contingency and mental retardation, and suggestions for future research are offered."}
{"id": "PMID:489513", "title": "Childhood psychosis in the preschool child.", "content": "Six cases of childhood psychosis in children 3 to 5 years of age are summarized in order to alert clinicians to include this clinical entity in the differential diagnosis of children with major developmental difficulties. They represent children treated in a day, nonresidential treatment facility in a suburb of New York City. These cases suggest that we reexamine the prevalent interest in a bipolar distribution of childhood psychosis that focuses on children whose psychosis occurs under 3 and over 5 years of age. Children with psychosis occurring between 3 and 5 are currently described as essentially rare, and are characterized by a path of massive deterioration. Our cases indicate that we should be alert to the phenomenon of young children with psychosis appearing at the preschool age, and in whom the course of illness is in a more positive direction.", "contents": "Childhood psychosis in the preschool child. Six cases of childhood psychosis in children 3 to 5 years of age are summarized in order to alert clinicians to include this clinical entity in the differential diagnosis of children with major developmental difficulties. They represent children treated in a day, nonresidential treatment facility in a suburb of New York City. These cases suggest that we reexamine the prevalent interest in a bipolar distribution of childhood psychosis that focuses on children whose psychosis occurs under 3 and over 5 years of age. Children with psychosis occurring between 3 and 5 are currently described as essentially rare, and are characterized by a path of massive deterioration. Our cases indicate that we should be alert to the phenomenon of young children with psychosis appearing at the preschool age, and in whom the course of illness is in a more positive direction."}
{"id": "PMID:489514", "title": "Measuring musical abilities of autistic children.", "content": "Three normal children with reported musical ability and three autistic children were tested for the ability to imitate individual tones and series of tones delivered by voice, piano, and synthesizer. Accuracy of imitation was judged by two independent observers on the basis of pitch, rhythm, and duration. The autistic children overall performed as well as or better than the age-matched normal children. These results are discussed and their implications for future neurological and clinical research are considered.", "contents": "Measuring musical abilities of autistic children. Three normal children with reported musical ability and three autistic children were tested for the ability to imitate individual tones and series of tones delivered by voice, piano, and synthesizer. Accuracy of imitation was judged by two independent observers on the basis of pitch, rhythm, and duration. The autistic children overall performed as well as or better than the age-matched normal children. These results are discussed and their implications for future neurological and clinical research are considered."}
{"id": "PMID:489517", "title": "Double-blind study of thioridazine and haloperidol in geriatric patients with a psychosis associated with organic brain syndrome.", "content": "Forty geriatric patients from the psychiatric ward of a state hospital were enrolled in a 12 week double-blind comparative study of the concentrate forms of thioridazine and haloperidol. The objective of the study was to assess the efficacy and safety of these drugs in the treatment of psychosis associated with organic brain syndrome in the elderly. Two types of patients comprised the population, those who had been hospitalized most of their adult lives and those who had not entered the hospital until late in life. Although both drugs produced significant improvement in these patients' symptoms, the improvement with thioridazine tended to be greater than that with haloperidol in most ratings. A plateau effect was seen with haloperidol in contrast to a steady improvement seen with thioridazine. The safety of both drugs was confirmed. Geriatric patients who display both psychotic and OBS symptomatology were found to respond quite well to both drugs, regardless of their previous psychiatric history, but a somewhat more dramatic response was seen with thioridazine.", "contents": "Double-blind study of thioridazine and haloperidol in geriatric patients with a psychosis associated with organic brain syndrome. Forty geriatric patients from the psychiatric ward of a state hospital were enrolled in a 12 week double-blind comparative study of the concentrate forms of thioridazine and haloperidol. The objective of the study was to assess the efficacy and safety of these drugs in the treatment of psychosis associated with organic brain syndrome in the elderly. Two types of patients comprised the population, those who had been hospitalized most of their adult lives and those who had not entered the hospital until late in life. Although both drugs produced significant improvement in these patients' symptoms, the improvement with thioridazine tended to be greater than that with haloperidol in most ratings. A plateau effect was seen with haloperidol in contrast to a steady improvement seen with thioridazine. The safety of both drugs was confirmed. Geriatric patients who display both psychotic and OBS symptomatology were found to respond quite well to both drugs, regardless of their previous psychiatric history, but a somewhat more dramatic response was seen with thioridazine."}
{"id": "PMID:489518", "title": "Self-mutilation of the eye.", "content": "While the act of self-enucleation is rare, other self-inflicted eye injuries may be more common. Six patients are reported who have documented histories of ocular self-mutilation. Several common factors are observed in these patients and in other cases reported in the literature: (1) the patient was judged to be psychotic; (2) the patient had a tyrannical conscience; (3) a source of guilt was present; (4) this guilt was displaced to the eye; and (5) an attempt at self-inflicted eye injury was followed by relief from anxiety when completed or by frustration when injury was prevented.", "contents": "Self-mutilation of the eye. While the act of self-enucleation is rare, other self-inflicted eye injuries may be more common. Six patients are reported who have documented histories of ocular self-mutilation. Several common factors are observed in these patients and in other cases reported in the literature: (1) the patient was judged to be psychotic; (2) the patient had a tyrannical conscience; (3) a source of guilt was present; (4) this guilt was displaced to the eye; and (5) an attempt at self-inflicted eye injury was followed by relief from anxiety when completed or by frustration when injury was prevented."}
{"id": "PMID:489519", "title": "Pathological intoxication--is there such an entity?", "content": "Pathological intoxication appears as a diagnostic term in DSM-II and DSM-III, is defined in a psychiatric dictionary and is described in several textbooks. Still, is there really such an entity? Is the small amount of alcohol inbibed incidental rather than causal? It should be borne in mind that alcohol is the popular explain-all of our culture and as such is used as an excuse for everything from sexual indiscretions to well-planned \"impulsive\" acts. It is likely--and evidence is presented for this conclusion--that so-called cases of pathological intoxication are really dissociative reactions. The existence of pathological intoxication as an entity must remain in doubt unless the disorder can be reproduced under controlled conditions.", "contents": "Pathological intoxication--is there such an entity? Pathological intoxication appears as a diagnostic term in DSM-II and DSM-III, is defined in a psychiatric dictionary and is described in several textbooks. Still, is there really such an entity? Is the small amount of alcohol inbibed incidental rather than causal? It should be borne in mind that alcohol is the popular explain-all of our culture and as such is used as an excuse for everything from sexual indiscretions to well-planned \"impulsive\" acts. It is likely--and evidence is presented for this conclusion--that so-called cases of pathological intoxication are really dissociative reactions. The existence of pathological intoxication as an entity must remain in doubt unless the disorder can be reproduced under controlled conditions."}
{"id": "PMID:489520", "title": "Transketolase activity in psychiatric patients.", "content": "The thiamine status of 42 physically healthy nonalcoholic psychiatric inpatients was evaluated by measuring transketolase activity in a hemolysate of their red blood cells, before and after addition of thiamine pyrophosphate to the hemolysate. Sixteen patients (38%) showed evidence of thiamine deficiency. Five of these patients had received thiamine-containing vitamins for a period of from 2 fo 47 days prior to testing, suggesting that poor nutrition may not fully account for the thiamine deficiency observed.", "contents": "Transketolase activity in psychiatric patients. The thiamine status of 42 physically healthy nonalcoholic psychiatric inpatients was evaluated by measuring transketolase activity in a hemolysate of their red blood cells, before and after addition of thiamine pyrophosphate to the hemolysate. Sixteen patients (38%) showed evidence of thiamine deficiency. Five of these patients had received thiamine-containing vitamins for a period of from 2 fo 47 days prior to testing, suggesting that poor nutrition may not fully account for the thiamine deficiency observed."}
{"id": "PMID:489521", "title": "Insight and adherence to medication in chronic schizophrenics.", "content": "Chronic schizophrenics are well-known for the tendency to not take medication. Clinical observation and studies indicate that insight plays a significant role in medication adherence. This study investigated the roles of insight and perceived benefits from medication by patients. Those patients who had insight, perceived benefits from medication and also perceived a relation between the 2 were more likely to take medication than those who did not have insight nor perceive benefits. A speculative model and possible methods of increasing medication adherence are suggested.", "contents": "Insight and adherence to medication in chronic schizophrenics. Chronic schizophrenics are well-known for the tendency to not take medication. Clinical observation and studies indicate that insight plays a significant role in medication adherence. This study investigated the roles of insight and perceived benefits from medication by patients. Those patients who had insight, perceived benefits from medication and also perceived a relation between the 2 were more likely to take medication than those who did not have insight nor perceive benefits. A speculative model and possible methods of increasing medication adherence are suggested."}
{"id": "PMID:489522", "title": "Electromyograph biofeedback as an adjunct to standard psychiatric treatment.", "content": "The effectiveness of electromyograph (EMG) biofeedback training was studied among 2 groups of psychiatric patients, and a control group of normal subjects. Both the psychiatric patients and the normal subjects received 6 sessions of training in deep muscle relaxation and biofeedback. The schizophrenic and psychoneurotic patients had significantly lower ratings for pathological symptomatology and abnormal behavior and all groups significantly reduced their level of muscle tension following the treatment; the schizophrenics and normals displayed significantly greater reductions than the psychoneurotic patients. The length of hospitalization for the 2 groups of patients in the study was lower than for similar patients who received standard psychiatric treatment but no biofeedback training. These results suggest that EMG training can be a beneficial adjunctive treatment for severely disturbed patients.", "contents": "Electromyograph biofeedback as an adjunct to standard psychiatric treatment. The effectiveness of electromyograph (EMG) biofeedback training was studied among 2 groups of psychiatric patients, and a control group of normal subjects. Both the psychiatric patients and the normal subjects received 6 sessions of training in deep muscle relaxation and biofeedback. The schizophrenic and psychoneurotic patients had significantly lower ratings for pathological symptomatology and abnormal behavior and all groups significantly reduced their level of muscle tension following the treatment; the schizophrenics and normals displayed significantly greater reductions than the psychoneurotic patients. The length of hospitalization for the 2 groups of patients in the study was lower than for similar patients who received standard psychiatric treatment but no biofeedback training. These results suggest that EMG training can be a beneficial adjunctive treatment for severely disturbed patients."}
{"id": "PMID:489523", "title": "An Isakower phenomenon variant in an initital dream.", "content": "The Isakower phenomenon is reviewed, and a psychotherapy case is described. The first 2 dreams of the therapy are felt to be variants of the Isakower phenomenon, and are believed to support several hypotheses in the literature concerning the phenomenon.", "contents": "An Isakower phenomenon variant in an initital dream. The Isakower phenomenon is reviewed, and a psychotherapy case is described. The first 2 dreams of the therapy are felt to be variants of the Isakower phenomenon, and are believed to support several hypotheses in the literature concerning the phenomenon."}
{"id": "PMID:489524", "title": "Brief reactive psychosis: a psychodynamic interpretation.", "content": "The diagnosis \"brief reactive psychosis\" leaves little implied in the way of etiology or treatment approach. Three case histories are presented to illustrate the term, followed by brief summaries of the major dynamics involved. The older diagnostic term \"hysterical psychosis\" is discussed, and a parallel is drawn between this form of brief reactive psychosis and others so that they may be psychodynamically understood and that treatment may be rationally based upon this model.", "contents": "Brief reactive psychosis: a psychodynamic interpretation. The diagnosis \"brief reactive psychosis\" leaves little implied in the way of etiology or treatment approach. Three case histories are presented to illustrate the term, followed by brief summaries of the major dynamics involved. The older diagnostic term \"hysterical psychosis\" is discussed, and a parallel is drawn between this form of brief reactive psychosis and others so that they may be psychodynamically understood and that treatment may be rationally based upon this model."}
{"id": "PMID:489525", "title": "Case studies in neuropsychiaatry. II: Conversion pseudodementia.", "content": "A patient is described who experienced pseudodementia as a conversion reaction. The patient was considered to be demented originally because of the evidence for profound cognitive impairment elicited on mental state examination. Careful analysis of the patient's verbal production  in unstructured situations and of her behavior inthe hospital permitted a clinical diagnosis of pseudodementia to be made, a diagnosis confirmed by response to treatment and long term follow-up. Evidence is presented to support the interpretation of her pseudodementia as a conversion reaction.", "contents": "Case studies in neuropsychiaatry. II: Conversion pseudodementia. A patient is described who experienced pseudodementia as a conversion reaction. The patient was considered to be demented originally because of the evidence for profound cognitive impairment elicited on mental state examination. Careful analysis of the patient's verbal production  in unstructured situations and of her behavior inthe hospital permitted a clinical diagnosis of pseudodementia to be made, a diagnosis confirmed by response to treatment and long term follow-up. Evidence is presented to support the interpretation of her pseudodementia as a conversion reaction."}
{"id": "PMID:489527", "title": "Renal damage associated with long term use of lithium carbonate.", "content": "The authors report 2 cases of renal damage associated with lithium carbonate treatment. The relevant literature is reviewed and supports the claim that the lithium ion may be nephrotoxic. The article concludes with practical recommendations for clinical practice until futher evidence regarding this association is available.", "contents": "Renal damage associated with long term use of lithium carbonate. The authors report 2 cases of renal damage associated with lithium carbonate treatment. The relevant literature is reviewed and supports the claim that the lithium ion may be nephrotoxic. The article concludes with practical recommendations for clinical practice until futher evidence regarding this association is available."}
{"id": "PMID:489528", "title": "Patterns of drug use in psychiatric inpatient wards.", "content": "Drug use was monitored in a total of 2,592 psychiatric inpatients in 6 hospitals. The efficacy was rated as satisfactory in 78% of the drug exposures. Nearly 37% of the patients had an adverse reaction. However, only 1.5% of the reactions were noted as being of major severity.", "contents": "Patterns of drug use in psychiatric inpatient wards. Drug use was monitored in a total of 2,592 psychiatric inpatients in 6 hospitals. The efficacy was rated as satisfactory in 78% of the drug exposures. Nearly 37% of the patients had an adverse reaction. However, only 1.5% of the reactions were noted as being of major severity."}
{"id": "PMID:489529", "title": "The early manifestations of adolescent thought disorder.", "content": "Clinicians who wait for disturbed adolescents to fulfil the diagnostic criteria for schizophrenia or a toxic psychosis will delay diagnosing a developing thought disorder until the youngster has been taught to be crazy and has become chronically stunted psychologically. Earlier diagnosis can allow more prompt prevention and support and can facilitate treatment and minimize handicapping. No single symptom is diagnostic of a developing thought disorder but thought disruption can be suspected when a cluster of persistent symptoms is present. These early manifestations of teenage thought disorder are presented and discussed.", "contents": "The early manifestations of adolescent thought disorder. Clinicians who wait for disturbed adolescents to fulfil the diagnostic criteria for schizophrenia or a toxic psychosis will delay diagnosing a developing thought disorder until the youngster has been taught to be crazy and has become chronically stunted psychologically. Earlier diagnosis can allow more prompt prevention and support and can facilitate treatment and minimize handicapping. No single symptom is diagnostic of a developing thought disorder but thought disruption can be suspected when a cluster of persistent symptoms is present. These early manifestations of teenage thought disorder are presented and discussed."}
{"id": "PMID:489530", "title": "The significance of the serum levels of vitamin B-1 and magnesium in delirium tremens and alcoholism.", "content": "The relation between serum levels of vitamin B-1 and magnesium at admission, and the clinical state was studied in hospitalized patients with either alcoholism or delirium tremens. Vitamin B-1 was deficient in the delirium group, magnesium in both groups. There was no correlation between vitamin B-1 and magnesium levels. Patients with an unfavorable outcome at discharge had lower vitamine B-1 levels and a higher age than patients with a favorable outcome in either group. The magnesium deficiency is discussed for longstanding malnutrition in both groups, the vitamin B-1 deficiency for an unusual high intake of alcohol before delirium tremens started.", "contents": "The significance of the serum levels of vitamin B-1 and magnesium in delirium tremens and alcoholism. The relation between serum levels of vitamin B-1 and magnesium at admission, and the clinical state was studied in hospitalized patients with either alcoholism or delirium tremens. Vitamin B-1 was deficient in the delirium group, magnesium in both groups. There was no correlation between vitamin B-1 and magnesium levels. Patients with an unfavorable outcome at discharge had lower vitamine B-1 levels and a higher age than patients with a favorable outcome in either group. The magnesium deficiency is discussed for longstanding malnutrition in both groups, the vitamin B-1 deficiency for an unusual high intake of alcohol before delirium tremens started."}
{"id": "PMID:489531", "title": "Anorexia nervosa and mental retardation: a case report.", "content": "This is the first published report of a retarded individual developing anorexia nervosa. The authors discuss the interaction between subnormal intelligence and the presentation of anorexic symptomatology. They also discuss the relationship between this disorder and depression. Anorexia nervosa may not be uncommon in retarded adolescents but may go undiagnosed because of the belief that mentally retarded individuals do not develop this disorder.", "contents": "Anorexia nervosa and mental retardation: a case report. This is the first published report of a retarded individual developing anorexia nervosa. The authors discuss the interaction between subnormal intelligence and the presentation of anorexic symptomatology. They also discuss the relationship between this disorder and depression. Anorexia nervosa may not be uncommon in retarded adolescents but may go undiagnosed because of the belief that mentally retarded individuals do not develop this disorder."}
{"id": "PMID:489534", "title": "Investigations on myelination in vitro. Regulation by thyroid hormone in cultures of dissociated brain cells from embryonic mice.", "content": "Cultures of dissociated brain cells from embryonic mice were used to study the influence of thyroid hormone on myelination in vitro. Synthesis of myelin-associated lipids such as cerebrosides and sulfatides was used as an index for myelination. An experimental design, in which the cells were grown on medium containing serum from a thyroidectomized calf, was employed to demonstrate the direct effect of L-3,5,3'-triiodothyronine (T3Y on the biosynthesis of myelin lipids. The cells grown in the presence of hypothyroid calf serum which contained very low levels of thyroid hormones (T4 (thyroxine), 1.2 microgram/ml; T3, less than 25 ng/100 ml) compared to normal serum (T4, 5.8 microgram/ml; T3, 110 ng/100 ml) showed a diminished synthesis of myelin-associated glycolipids. This reduced activity could be restored to normal by including T3 (13 ng/ml) in the medium.", "contents": "Investigations on myelination in vitro. Regulation by thyroid hormone in cultures of dissociated brain cells from embryonic mice. Cultures of dissociated brain cells from embryonic mice were used to study the influence of thyroid hormone on myelination in vitro. Synthesis of myelin-associated lipids such as cerebrosides and sulfatides was used as an index for myelination. An experimental design, in which the cells were grown on medium containing serum from a thyroidectomized calf, was employed to demonstrate the direct effect of L-3,5,3'-triiodothyronine (T3Y on the biosynthesis of myelin lipids. The cells grown in the presence of hypothyroid calf serum which contained very low levels of thyroid hormones (T4 (thyroxine), 1.2 microgram/ml; T3, less than 25 ng/100 ml) compared to normal serum (T4, 5.8 microgram/ml; T3, 110 ng/100 ml) showed a diminished synthesis of myelin-associated glycolipids. This reduced activity could be restored to normal by including T3 (13 ng/ml) in the medium."}
{"id": "PMID:489535", "title": "7-Methylguanosine-dependent inhibition of globin mRNA translation by methylglyoxal.", "content": "Studies on the inhibition of translation by methylglyoxal of capped and chemically decapped globin mRNAs in the rabbit reciculocyte system strongly suggest that it is cap-dependent. Concentrations of methylglyoxal (0.2 mM), which effected substantial inhibition (80%) of capped mRNA, were only slightly inhibitory (10%) to the decapped species. In addition, the inhibition was K+-dependent with maximal inhibition occurring at the K+ optimum for translation of the capped species, suggesting cap recognition is required for the effect. Results with endogenous mRNA further substantiate that initiation and not elongation is the site of action. These results are consistent with an inhibition due to a newly discovered, rapid reaction of methylglyoxal with the 7-methylguanosine of the cap structure.", "contents": "7-Methylguanosine-dependent inhibition of globin mRNA translation by methylglyoxal. Studies on the inhibition of translation by methylglyoxal of capped and chemically decapped globin mRNAs in the rabbit reciculocyte system strongly suggest that it is cap-dependent. Concentrations of methylglyoxal (0.2 mM), which effected substantial inhibition (80%) of capped mRNA, were only slightly inhibitory (10%) to the decapped species. In addition, the inhibition was K+-dependent with maximal inhibition occurring at the K+ optimum for translation of the capped species, suggesting cap recognition is required for the effect. Results with endogenous mRNA further substantiate that initiation and not elongation is the site of action. These results are consistent with an inhibition due to a newly discovered, rapid reaction of methylglyoxal with the 7-methylguanosine of the cap structure."}
{"id": "PMID:489536", "title": "Platelet-activating factor. Evidence for 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators).", "content": "A glyceryl ether containing phosphoglyceride, 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (Ac-GEPC), has been shown to have a biological activity indistinguishable from that of naturally generated (rabbit) platelet activating factor (PAF). Its biochemical and biological properties so closely parallel those of naturally occurring PAF that we propose they are one and the same compound. Both PAF and AcGEPC could be converted to an inactive form through base-catalyzed methanolysis and restored to 100% functional activity by reaction with acetic anhydride. The synthetic lipid, AcGEPC, elicited 50% secretion of serotonin from rabbit platelets at a level of 10(-10) M (based on phosphorus). A propionyl derivative had somewhat comparable activity towards platelets, whereas the butyryl homologue was some 7-fold less active and the stearoyl derivative was inactive. These short chain acylglyceryl ether phosphoglycerides represent an entirely new, potent and unique class of lipid chemical mediators. 1-Acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (AcLL) also exhibited activity towards platelets but was some 200-fold less active than AcGepc. the propionyl lysolecithin behaved quite similarly to AcLL, but butyryl and stearoyl lysolecithins showed no activity.", "contents": "Platelet-activating factor. Evidence for 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators). A glyceryl ether containing phosphoglyceride, 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (Ac-GEPC), has been shown to have a biological activity indistinguishable from that of naturally generated (rabbit) platelet activating factor (PAF). Its biochemical and biological properties so closely parallel those of naturally occurring PAF that we propose they are one and the same compound. Both PAF and AcGEPC could be converted to an inactive form through base-catalyzed methanolysis and restored to 100% functional activity by reaction with acetic anhydride. The synthetic lipid, AcGEPC, elicited 50% secretion of serotonin from rabbit platelets at a level of 10(-10) M (based on phosphorus). A propionyl derivative had somewhat comparable activity towards platelets, whereas the butyryl homologue was some 7-fold less active and the stearoyl derivative was inactive. These short chain acylglyceryl ether phosphoglycerides represent an entirely new, potent and unique class of lipid chemical mediators. 1-Acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (AcLL) also exhibited activity towards platelets but was some 200-fold less active than AcGepc. the propionyl lysolecithin behaved quite similarly to AcLL, but butyryl and stearoyl lysolecithins showed no activity."}
{"id": "PMID:489537", "title": "Purification of riboflavin-binding proteins from bovine plasma and discovery of a pregnancy-specific riboflavin-binding protein.", "content": "Riboflavin-binding proteins have been purified from bovine plasma using flavinyl agarose beads. At least three major protein bands, migrating in regions assigned to the beta- and gamma-globulins of plasma, are observed by cellulose acetate electrophoresis. These proteins coelute from a calibrated Sephadex G-100 column in the volume corresponding to a molecular weight of approximately 150,000; a small amount of another riboflavin-binding protein (molecular weight approximately 37,000) is also present. Polyacrylamide gel electrophoresis of the proteins,  with detection by autoradiography of those having tightly bound [2-14C]riboflavin, reveals one protein band which is present only in preparations from pregnant cows. This protein has been purified to apparent homogeneity by storing the mixture of riboflavin-binding proteins at 8 degrees C for 3 weeks, which precipitates the other, less stable proteins. Hence, bovine plasma, like that of the laying hen, contains a number of riboflavin-binding proteins, one of which correlates with pregnancy.", "contents": "Purification of riboflavin-binding proteins from bovine plasma and discovery of a pregnancy-specific riboflavin-binding protein. Riboflavin-binding proteins have been purified from bovine plasma using flavinyl agarose beads. At least three major protein bands, migrating in regions assigned to the beta- and gamma-globulins of plasma, are observed by cellulose acetate electrophoresis. These proteins coelute from a calibrated Sephadex G-100 column in the volume corresponding to a molecular weight of approximately 150,000; a small amount of another riboflavin-binding protein (molecular weight approximately 37,000) is also present. Polyacrylamide gel electrophoresis of the proteins,  with detection by autoradiography of those having tightly bound [2-14C]riboflavin, reveals one protein band which is present only in preparations from pregnant cows. This protein has been purified to apparent homogeneity by storing the mixture of riboflavin-binding proteins at 8 degrees C for 3 weeks, which precipitates the other, less stable proteins. Hence, bovine plasma, like that of the laying hen, contains a number of riboflavin-binding proteins, one of which correlates with pregnancy."}
{"id": "PMID:489538", "title": "Phosphorylation of ATP citrate lyase in response to glucagon.", "content": "Incubation of hepatocytes with [32P]orthophosphate resulted in the incorporation of 32P into material that is precipitated by reaction with antibodies to ATP citrate lyase. The amount of radioactivity precipitated was decreased when unlabeled, purified ATP citrate lyase was added to extracts of hepatocytes that had been incubated with [32P]orthophosphate. Addition of glucagon to hepatocytes that had been preincubated with [32P]orthophosphate resulted in a 56% increase in acid-stable 32P in the trichloroacetic acid-insoluble portion of immunoprecipitates. Catalytic phosphate bound to ATP citrate lyase reaction with ATP and Mg2+ is acid-labile; thus, glucagon-dependent phosphorylation is distinguished from the catalytic phosphate. When hepatocytes were incubated in the absence of [32P]orthophosphate and extracted in a medium containing [gamma-32P]ATP, no acid-stable 32P was present in immunoprecipitates. This indicates that the incorporation into ATP citrate lyase of acid-stable phosphate occurs prior to extraction of the enzyme. Preliminary studies, using a procedure that allows for measurement of enzyme activity starting 1 min after beginning the extraction of lyase from hepatocytes, have shown no difference in lyase activity when hepatocytes are treated with or without glucagon.", "contents": "Phosphorylation of ATP citrate lyase in response to glucagon. Incubation of hepatocytes with [32P]orthophosphate resulted in the incorporation of 32P into material that is precipitated by reaction with antibodies to ATP citrate lyase. The amount of radioactivity precipitated was decreased when unlabeled, purified ATP citrate lyase was added to extracts of hepatocytes that had been incubated with [32P]orthophosphate. Addition of glucagon to hepatocytes that had been preincubated with [32P]orthophosphate resulted in a 56% increase in acid-stable 32P in the trichloroacetic acid-insoluble portion of immunoprecipitates. Catalytic phosphate bound to ATP citrate lyase reaction with ATP and Mg2+ is acid-labile; thus, glucagon-dependent phosphorylation is distinguished from the catalytic phosphate. When hepatocytes were incubated in the absence of [32P]orthophosphate and extracted in a medium containing [gamma-32P]ATP, no acid-stable 32P was present in immunoprecipitates. This indicates that the incorporation into ATP citrate lyase of acid-stable phosphate occurs prior to extraction of the enzyme. Preliminary studies, using a procedure that allows for measurement of enzyme activity starting 1 min after beginning the extraction of lyase from hepatocytes, have shown no difference in lyase activity when hepatocytes are treated with or without glucagon."}
{"id": "PMID:489539", "title": "Radioimmunoassay of the link proteins associated with bovine nasal cartilage proteoglycan.", "content": "Link proteins from bovine nasal cartilage have been purified by preparative polyacrylamide gel electrophoresis in sodium dodecyl sulfate (Baker, J.R., and Caterson, B. (1979) J. Biol. Chem. 254, 2387-2393) and used to raise antisera in rabbits. A sensitive radioimmunoassay procedure utilizing binding of 125I-labeled antigen . antibody complexes to Protein A of Staphylococcus aureus has served to demonstrate the specificity of the antisera for the link proteins. The lack of reactivity with proteoglycan fractions indicates that link proteins and proteoglycan do not share antigenic determinants. This result is in accord with published cyanogen bromide peptide cleavage data (Baker, J.R., and Caterson B. (1977) Biochem. Biophys. Res. Commun. 77, 1-10) which showed proteoglycan and link protein to be structurally dissimilar. The radioimmunoassay procedure has been used to quantitate small amounts of link protein which remain associated with proteoglycan after purification by equilibrium density gradient centrifugation in 4 M guanidine HCl and by gel chromatography in sodium dodecyl sulfate.", "contents": "Radioimmunoassay of the link proteins associated with bovine nasal cartilage proteoglycan. Link proteins from bovine nasal cartilage have been purified by preparative polyacrylamide gel electrophoresis in sodium dodecyl sulfate (Baker, J.R., and Caterson, B. (1979) J. Biol. Chem. 254, 2387-2393) and used to raise antisera in rabbits. A sensitive radioimmunoassay procedure utilizing binding of 125I-labeled antigen . antibody complexes to Protein A of Staphylococcus aureus has served to demonstrate the specificity of the antisera for the link proteins. The lack of reactivity with proteoglycan fractions indicates that link proteins and proteoglycan do not share antigenic determinants. This result is in accord with published cyanogen bromide peptide cleavage data (Baker, J.R., and Caterson B. (1977) Biochem. Biophys. Res. Commun. 77, 1-10) which showed proteoglycan and link protein to be structurally dissimilar. The radioimmunoassay procedure has been used to quantitate small amounts of link protein which remain associated with proteoglycan after purification by equilibrium density gradient centrifugation in 4 M guanidine HCl and by gel chromatography in sodium dodecyl sulfate."}
{"id": "PMID:489541", "title": "Protein-catalyzed exchange of phosphatidylinositol between rat brain microsomes and myelin.", "content": "Exchange of phosphatidylinositol and phosphatidylcholine between microsomal and myelin membranes has been demonstrated. This exchange is reversible and catalyzed by soluble proteins from the brain homogenate precipitated at pH 5.1. The extent of exchange of phosphatidylinositol from microsomal membrane to myelin is dependent upon pH and temperature, with an optimum around pH 7 and at 50 degrees C. Maximum exchange was observed at approximately equal amounts of microsomal, myelin, and supernatant proteins. The extent of the catalyzed exchange increases 4- to 8-fold upon using sonicated or heat-treated myelin as an acceptor membrane. Heating of microsomal membranes results in no change. The extent of catalyzed exchange of phosphatidylcholine is less than that of the phosphatidylinositol. The exchange of other phospholipids and glycolipids between microsomal and myelin membranes cannot be demonstrated. The catalytic activity of the pH 5.1 supernatant proteins in rat brain for the exchange of phosphatidylinositol increases with age after birth and reaches a maximum around 21 days of age analogous to the process of myelination. The pH 5.1 supernatant proteins from quaking and jimpy mutant mice has normal catalytic activity.", "contents": "Protein-catalyzed exchange of phosphatidylinositol between rat brain microsomes and myelin. Exchange of phosphatidylinositol and phosphatidylcholine between microsomal and myelin membranes has been demonstrated. This exchange is reversible and catalyzed by soluble proteins from the brain homogenate precipitated at pH 5.1. The extent of exchange of phosphatidylinositol from microsomal membrane to myelin is dependent upon pH and temperature, with an optimum around pH 7 and at 50 degrees C. Maximum exchange was observed at approximately equal amounts of microsomal, myelin, and supernatant proteins. The extent of the catalyzed exchange increases 4- to 8-fold upon using sonicated or heat-treated myelin as an acceptor membrane. Heating of microsomal membranes results in no change. The extent of catalyzed exchange of phosphatidylcholine is less than that of the phosphatidylinositol. The exchange of other phospholipids and glycolipids between microsomal and myelin membranes cannot be demonstrated. The catalytic activity of the pH 5.1 supernatant proteins in rat brain for the exchange of phosphatidylinositol increases with age after birth and reaches a maximum around 21 days of age analogous to the process of myelination. The pH 5.1 supernatant proteins from quaking and jimpy mutant mice has normal catalytic activity."}
{"id": "PMID:489543", "title": "Kinetics of the activation of human prothrombin by human coagulation factor Xa. Initial rate studies in the presence of Ca2+ and phospholipid.", "content": "Steady state kinetic studies have been performed to investigate the formation of thrombin from prothrombin by human coagulation Factor Xa in the presence of Ca2+ and phospholipid. The concentration of ligand which gives 50% of the maximum velocity (K0.5) is 2.3 mM for Ca2+, 7.4 microM for phospholipid, and 0.006 microM for prothrombin. Hill plots of the Ca2+ enhancement of the reaction give a Hill coefficient of 3.1, indicating positive cooperativity. The initial velocity patterns are consistent with an ordered addition of reactants with phospholipid as the second reactant to bind to the enzyme. Although our results do not differentiate between Ca2+ or the prothrombin substrate as the first reactant to bind to Factor Xa, it is established that Ca2+ can bind to Factor Xa in the absence of the other reactants. Thus, the most probable order of addition of reactants is Ca2+, phospholipid, and the prothrombin substrate. Plots of (v)-1 versus (prothrombin)-1 or (v)-1 versus [(Ca2+)3]-1 at several constant concentrations of phospholipid indicate that the major effect of phospholipid is to increase the turnover number of Factor Xa.", "contents": "Kinetics of the activation of human prothrombin by human coagulation factor Xa. Initial rate studies in the presence of Ca2+ and phospholipid. Steady state kinetic studies have been performed to investigate the formation of thrombin from prothrombin by human coagulation Factor Xa in the presence of Ca2+ and phospholipid. The concentration of ligand which gives 50% of the maximum velocity (K0.5) is 2.3 mM for Ca2+, 7.4 microM for phospholipid, and 0.006 microM for prothrombin. Hill plots of the Ca2+ enhancement of the reaction give a Hill coefficient of 3.1, indicating positive cooperativity. The initial velocity patterns are consistent with an ordered addition of reactants with phospholipid as the second reactant to bind to the enzyme. Although our results do not differentiate between Ca2+ or the prothrombin substrate as the first reactant to bind to Factor Xa, it is established that Ca2+ can bind to Factor Xa in the absence of the other reactants. Thus, the most probable order of addition of reactants is Ca2+, phospholipid, and the prothrombin substrate. Plots of (v)-1 versus (prothrombin)-1 or (v)-1 versus [(Ca2+)3]-1 at several constant concentrations of phospholipid indicate that the major effect of phospholipid is to increase the turnover number of Factor Xa."}
{"id": "PMID:489545", "title": "Prenyltransferase. Kinetic studies of the 1'-4 coupling reaction with avian liver enzyme.", "content": "Prenyltransferase catalyzes the sequential, irreversible 1'-4 condensation of isopentenyl-PP with dimethylallyl-PP and geranyl-PP to yield farnesyl-PP. A kinetic study shows substrate inhibition by isopentenyl-PP at concentrations above 0.7 microM when the concentration of geranyl-PP is 1.0 microM or less as a result of binding by the homoallylic substrate to the allylic region of the active site. Inhibition studies were carried out with the products, farnesyl-PP and PPi, and dead-end inhibitors 2-F-isopentenyl-PP and 2-F-geranyl-PP, analogues for the normal substrates. Competitive patterns were seen for farnesyl-PP and 2-F-geranyl-PP when geranyl-PP was varied, while noncompetitive patterns were found for all other combinations. A minor form of PPi, MgHPPi-, is implicated as the species of PPi in the magnesium-containing buffer which binds most tightly to the enzyme. This observation explains why K's for PPi calculated from the total concentration of PPi are much larger than K's for the organic pyrophosphates. The lack of regiospecificity in the binding of isopentenyl-PP, as evidenced by substrate inhibition patterns, introduces an element of ambiguity into mechanistic interpretations, and it is not possible to distinguish between ordered and random mechanisms on the basis of inhibition studies.", "contents": "Prenyltransferase. Kinetic studies of the 1'-4 coupling reaction with avian liver enzyme. Prenyltransferase catalyzes the sequential, irreversible 1'-4 condensation of isopentenyl-PP with dimethylallyl-PP and geranyl-PP to yield farnesyl-PP. A kinetic study shows substrate inhibition by isopentenyl-PP at concentrations above 0.7 microM when the concentration of geranyl-PP is 1.0 microM or less as a result of binding by the homoallylic substrate to the allylic region of the active site. Inhibition studies were carried out with the products, farnesyl-PP and PPi, and dead-end inhibitors 2-F-isopentenyl-PP and 2-F-geranyl-PP, analogues for the normal substrates. Competitive patterns were seen for farnesyl-PP and 2-F-geranyl-PP when geranyl-PP was varied, while noncompetitive patterns were found for all other combinations. A minor form of PPi, MgHPPi-, is implicated as the species of PPi in the magnesium-containing buffer which binds most tightly to the enzyme. This observation explains why K's for PPi calculated from the total concentration of PPi are much larger than K's for the organic pyrophosphates. The lack of regiospecificity in the binding of isopentenyl-PP, as evidenced by substrate inhibition patterns, introduces an element of ambiguity into mechanistic interpretations, and it is not possible to distinguish between ordered and random mechanisms on the basis of inhibition studies."}
{"id": "PMID:489546", "title": "Superstructural differences between chromatin in nuclei and in solution are revealed by kinetics of micrococcal nuclease digestion.", "content": "Digestion of chromatin in nuclei by micrococcal nuclease, measured as the change in the concentration of monomer-length DNA with time, displays Michaelis-Menten kinetics. Redigestion of soluble chromatin prepared from nuclei by micrococcal nuclease treatment, however, is apparently first order in enzyme and independent of chromatin concentration. This qualitative difference results from an increase in the apparent second order rate constant, kcat/Km, for liberation of monomer DNA: the apparent Km for soluble chromatin is lower by close to 3 orders of magnitude than that for chromatin in nuclei, whereas kcat decreases by less than 1 order of magnitude. Neither the integrity of the nuclear membrane nor the presence of histone H1 contributes to the high Michaelis constant characteristic of chromatin in nuclei. Moreover, differences due to the buffers used for digestion and redigestion are minimal. Low catalytic efficiency is, however, correlated with the presence of higher order chromatin superstructure. Micrococcal nuclease added to soluble chromatin under nondigesting conditions at low ionic strength (I = 0.002) co-sediments with chromatin in sucrose gradients. In 0.15 M NaCl, added nuclease no longer sediments with chromatin and redigestion kinetics become first order in both enzyme and substrate. Kinetic analysis of this type may afford an assay for native, higher order structures in chromatin. Our results suggest that micrococcal nuclease binds to soluble chromatin through additional interactions not present in nuclei, which may be partly ionic in nature.", "contents": "Superstructural differences between chromatin in nuclei and in solution are revealed by kinetics of micrococcal nuclease digestion. Digestion of chromatin in nuclei by micrococcal nuclease, measured as the change in the concentration of monomer-length DNA with time, displays Michaelis-Menten kinetics. Redigestion of soluble chromatin prepared from nuclei by micrococcal nuclease treatment, however, is apparently first order in enzyme and independent of chromatin concentration. This qualitative difference results from an increase in the apparent second order rate constant, kcat/Km, for liberation of monomer DNA: the apparent Km for soluble chromatin is lower by close to 3 orders of magnitude than that for chromatin in nuclei, whereas kcat decreases by less than 1 order of magnitude. Neither the integrity of the nuclear membrane nor the presence of histone H1 contributes to the high Michaelis constant characteristic of chromatin in nuclei. Moreover, differences due to the buffers used for digestion and redigestion are minimal. Low catalytic efficiency is, however, correlated with the presence of higher order chromatin superstructure. Micrococcal nuclease added to soluble chromatin under nondigesting conditions at low ionic strength (I = 0.002) co-sediments with chromatin in sucrose gradients. In 0.15 M NaCl, added nuclease no longer sediments with chromatin and redigestion kinetics become first order in both enzyme and substrate. Kinetic analysis of this type may afford an assay for native, higher order structures in chromatin. Our results suggest that micrococcal nuclease binds to soluble chromatin through additional interactions not present in nuclei, which may be partly ionic in nature."}
{"id": "PMID:489547", "title": "Characterization of the 1 alpha,25-dihydroxyvitamin D3 receptor from rat intestinal cytosol.", "content": "The 1 alpha,25-dihydroxyvitamin D3 receptor from rat intestinal cytosol has been partially characterized. Sucrose density gradient sedimentation and analytical gel filtration analyses of this receptor yielded values of 3.1 S, 80,000, and 36 A for the sedimentation coefficient, molecular weight (Mr), and Stokes molecular radius (Rs), respectively. The receptor was found to be a protein by its susceptibility to protease but not nuclease digestion, and studies with N-ethylmaleimide and iodoacetamide revealed the presence of a reduced cysteine residue near the ligand binding site of the receptor. Kinetic and equilibrium binding studies showed an equilibrium dissociation constant of 7.4 x 10(-10) M (4 degrees C), an association rate constant of 1.7 x 10(7) M-1 min-1 (0 degrees C) and a dissociation rate constant of 7.2 x 10(-4) min-1 (4 degrees C, t1/2 = 16 h).", "contents": "Characterization of the 1 alpha,25-dihydroxyvitamin D3 receptor from rat intestinal cytosol. The 1 alpha,25-dihydroxyvitamin D3 receptor from rat intestinal cytosol has been partially characterized. Sucrose density gradient sedimentation and analytical gel filtration analyses of this receptor yielded values of 3.1 S, 80,000, and 36 A for the sedimentation coefficient, molecular weight (Mr), and Stokes molecular radius (Rs), respectively. The receptor was found to be a protein by its susceptibility to protease but not nuclease digestion, and studies with N-ethylmaleimide and iodoacetamide revealed the presence of a reduced cysteine residue near the ligand binding site of the receptor. Kinetic and equilibrium binding studies showed an equilibrium dissociation constant of 7.4 x 10(-10) M (4 degrees C), an association rate constant of 1.7 x 10(7) M-1 min-1 (0 degrees C) and a dissociation rate constant of 7.2 x 10(-4) min-1 (4 degrees C, t1/2 = 16 h)."}
{"id": "PMID:489548", "title": "Effect of thyroid hormone on the turnover of rat liver pyruvate carboxylase and pyruvate dehydrogenase.", "content": "Immunochemical techniques have been utilized to study the effect of thyroid status on the content and rates of synthesis and degradation of pyruvate carboxylase and pyruvate dehydrogenase in rat liver. Liver from hyperthyroid rats had twice the pyruvate carboxylase activity of normal rats while thyroidectomized rats had about two-thirds of normal activity. Pyruvate dehydrogenase complex activity was unchanged in the hyperthyroid state but was significantly reduced (by a third) in hypothyroid rats. Changes in catalytic activity during altered thyroid status were by immunochemical means to be closely related to the amount of the hepatic enzymes present. Isotopic studies showed that the changes in the content of pyruvate carboxylase and pyruvate dehydrogenase reflected alterations in the rate of the synthesis of the enzymes with the degradation rates little affected by thyroid status. The half-life for pyruvate carboxylase was 4.6 days, and that for pyruvate dehydrogenase, 8.1 days. In both cases, the turnover time was slower than that of the average mitochondrial protein (t1/2 = 3.8 days) for the control animals.", "contents": "Effect of thyroid hormone on the turnover of rat liver pyruvate carboxylase and pyruvate dehydrogenase. Immunochemical techniques have been utilized to study the effect of thyroid status on the content and rates of synthesis and degradation of pyruvate carboxylase and pyruvate dehydrogenase in rat liver. Liver from hyperthyroid rats had twice the pyruvate carboxylase activity of normal rats while thyroidectomized rats had about two-thirds of normal activity. Pyruvate dehydrogenase complex activity was unchanged in the hyperthyroid state but was significantly reduced (by a third) in hypothyroid rats. Changes in catalytic activity during altered thyroid status were by immunochemical means to be closely related to the amount of the hepatic enzymes present. Isotopic studies showed that the changes in the content of pyruvate carboxylase and pyruvate dehydrogenase reflected alterations in the rate of the synthesis of the enzymes with the degradation rates little affected by thyroid status. The half-life for pyruvate carboxylase was 4.6 days, and that for pyruvate dehydrogenase, 8.1 days. In both cases, the turnover time was slower than that of the average mitochondrial protein (t1/2 = 3.8 days) for the control animals."}
{"id": "PMID:489550", "title": "Increased ATP and creatine phosphate turnover in phagocytosing mouse peritoneal macrophages.", "content": "Resident and thioglycollate-elicited macrophages maintained in culture for 24 h contain approximately 5 x 10(-16) and 12 x 10(-16) mol of ATP per cell, respectively. During particle ingestion, the levels of ATP in these cells did not change. However, the specific activity of ATP extracted from macrophages labeled with [32P]Pi during phagocytosis was 40% lower than ATP extracted from control cells. These results suggested that macrophages contain a high energy phosphate reservoir, in addition to the ATP pool(s). A search for such a reservoir led to the identification of creatine phosphate in both resident and thioglycollate-elicited macrophages at concentrations that are in 3- to 5-fold-molar excess over ATP. Creatine phosphate levels in phagocytosing resident macrophages decreased by 45%, while creatine phosphate levels in phagocytosing thioglycollate-elicited macrophages did not change. Creatine phosphate turnover was measured in macrophages prelabeled with [14C]creatine. Over 90% of the intracellular label was in the form of creatine phosphate. During phagocytosis, there was a 40% decrease in intracellular [14C]creatine phosphate in both resident and thioglycollate-elicited macrophages. These results indicate that creatine phosphate turns over more rapidly during phagocytosis and replenishes the ATP consumed.", "contents": "Increased ATP and creatine phosphate turnover in phagocytosing mouse peritoneal macrophages. Resident and thioglycollate-elicited macrophages maintained in culture for 24 h contain approximately 5 x 10(-16) and 12 x 10(-16) mol of ATP per cell, respectively. During particle ingestion, the levels of ATP in these cells did not change. However, the specific activity of ATP extracted from macrophages labeled with [32P]Pi during phagocytosis was 40% lower than ATP extracted from control cells. These results suggested that macrophages contain a high energy phosphate reservoir, in addition to the ATP pool(s). A search for such a reservoir led to the identification of creatine phosphate in both resident and thioglycollate-elicited macrophages at concentrations that are in 3- to 5-fold-molar excess over ATP. Creatine phosphate levels in phagocytosing resident macrophages decreased by 45%, while creatine phosphate levels in phagocytosing thioglycollate-elicited macrophages did not change. Creatine phosphate turnover was measured in macrophages prelabeled with [14C]creatine. Over 90% of the intracellular label was in the form of creatine phosphate. During phagocytosis, there was a 40% decrease in intracellular [14C]creatine phosphate in both resident and thioglycollate-elicited macrophages. These results indicate that creatine phosphate turns over more rapidly during phagocytosis and replenishes the ATP consumed."}
{"id": "PMID:489551", "title": "Regulation of cholesterol synthesis in primary rat hepatocyte culture cells. Possible regulatory site at sterol demethylation.", "content": "Primary rat hepatocyte culture cells were used to study the acute regulation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity in response to 25-hydroxycholesterol, 3 beta,5 alpha,6 beta-cholestantriol, and mevalonolactone. All three effectors caused a rapid suppression of HMG-CoA reductase activity. 25-Hydroxycholesterol also caused an increase in the ratio of newly synthesized methyl sterols to newly synthesized C27-sterols. Furthermore, in 25-hydroxycholesterol-treated cells, the relative contribution of delta 24-sterol precursors to the nonsaponifiable lipid fraction increased. Di- and trimethyl-diene sterols were the dominant methyl sterols synthesized in the presence of 25-hydroxycholesterol. 3 beta,5 alpha,6 beta-Cholestrantriol (50 microM) also caused a very strong (97%) suppression of sterol demethylation; 4,4-dimethylmonoene sterols were more prominent (23%) in cells treated with 3 beta,5 alpha,6 beta-cholestrantriol, than in cells treated with 25-hydroxycholesterol (2%). The rates of both unesterified and esterified sterol synthesis increased as a function of exogenous mevalonolactone concentration. C27-sterol synthesis was saturated at a concentration of (R)-mevalonolactone which produced only a 33% suppression of HMG-CoA reductase activity. However, there was a direct relationship between the accumulation of methyl sterols and the decrease in HMG-CoA reductase activity. With the aid of triparanol, it was demonstrated that the suppression of HMG-CoA reductase activity by mevalonolactone was linked with the ability of the cells to convert squalene-2,3-epoxide into sterols. The results described in the present article support an important and perhaps necessary relationship between the rate of methyl sterol conversion of C27-sterols and the suppression or inhibition of HMG-Coa reductase in primary hepatocyte culture cells.", "contents": "Regulation of cholesterol synthesis in primary rat hepatocyte culture cells. Possible regulatory site at sterol demethylation. Primary rat hepatocyte culture cells were used to study the acute regulation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity in response to 25-hydroxycholesterol, 3 beta,5 alpha,6 beta-cholestantriol, and mevalonolactone. All three effectors caused a rapid suppression of HMG-CoA reductase activity. 25-Hydroxycholesterol also caused an increase in the ratio of newly synthesized methyl sterols to newly synthesized C27-sterols. Furthermore, in 25-hydroxycholesterol-treated cells, the relative contribution of delta 24-sterol precursors to the nonsaponifiable lipid fraction increased. Di- and trimethyl-diene sterols were the dominant methyl sterols synthesized in the presence of 25-hydroxycholesterol. 3 beta,5 alpha,6 beta-Cholestrantriol (50 microM) also caused a very strong (97%) suppression of sterol demethylation; 4,4-dimethylmonoene sterols were more prominent (23%) in cells treated with 3 beta,5 alpha,6 beta-cholestrantriol, than in cells treated with 25-hydroxycholesterol (2%). The rates of both unesterified and esterified sterol synthesis increased as a function of exogenous mevalonolactone concentration. C27-sterol synthesis was saturated at a concentration of (R)-mevalonolactone which produced only a 33% suppression of HMG-CoA reductase activity. However, there was a direct relationship between the accumulation of methyl sterols and the decrease in HMG-CoA reductase activity. With the aid of triparanol, it was demonstrated that the suppression of HMG-CoA reductase activity by mevalonolactone was linked with the ability of the cells to convert squalene-2,3-epoxide into sterols. The results described in the present article support an important and perhaps necessary relationship between the rate of methyl sterol conversion of C27-sterols and the suppression or inhibition of HMG-Coa reductase in primary hepatocyte culture cells."}
{"id": "PMID:489554", "title": "Inactivation of bovine pancreatic DNase by 2-nitro-5-thiocyanobenzoic acid. I. A novel inhibitor for DNase I.", "content": "Though DNase does not contain any cysteine residues, incubation of the enzyme with 2-nitro-5-thiocyanobenzoic acid in the presence of Ca2+ at pH values above 7.5 results in an irreversible inactivation of the enzyme. The inactivation also occurs when Ca2+ is replaced by Mg2+, but not in their absence. Amino acid analyses after acid hydrolyses of the completely inactivated ant the native enzymes show no significant differences in composition, including tryptophan and half-cystine residues. However, sodium dodecyl sulfate gel electrophoresis indicates enzyme cleavage by the treatment with 2-nitro-5-thiocyanobenzoic acid. This reagent does not inactivate chymotrypsin and lysozyme, and under conditions where bovine DNase is inactivated, does not inactivate other nucleases such as ribonuclease, snake venom phosphodiesterase, and spleen acid DNase. However, it inactivates malt DNase and can, therefore, be considered a specific inhibitor of DNase I. The inactivation kinetics is pseudo-first order, resembling Michaelis-Menten, with an affinity constant of 16.7 mM. It is the cyano group, not the thionitrobenzoic acid of 2-nitro-5-thiocyanobenzoic acid that reacts to form cyano-DNase.", "contents": "Inactivation of bovine pancreatic DNase by 2-nitro-5-thiocyanobenzoic acid. I. A novel inhibitor for DNase I. Though DNase does not contain any cysteine residues, incubation of the enzyme with 2-nitro-5-thiocyanobenzoic acid in the presence of Ca2+ at pH values above 7.5 results in an irreversible inactivation of the enzyme. The inactivation also occurs when Ca2+ is replaced by Mg2+, but not in their absence. Amino acid analyses after acid hydrolyses of the completely inactivated ant the native enzymes show no significant differences in composition, including tryptophan and half-cystine residues. However, sodium dodecyl sulfate gel electrophoresis indicates enzyme cleavage by the treatment with 2-nitro-5-thiocyanobenzoic acid. This reagent does not inactivate chymotrypsin and lysozyme, and under conditions where bovine DNase is inactivated, does not inactivate other nucleases such as ribonuclease, snake venom phosphodiesterase, and spleen acid DNase. However, it inactivates malt DNase and can, therefore, be considered a specific inhibitor of DNase I. The inactivation kinetics is pseudo-first order, resembling Michaelis-Menten, with an affinity constant of 16.7 mM. It is the cyano group, not the thionitrobenzoic acid of 2-nitro-5-thiocyanobenzoic acid that reacts to form cyano-DNase."}
{"id": "PMID:489555", "title": "Inactivation of DNase by 2-nitro-5-thiocyanobenzoic acid. II. Serine and threonine are the sites of reaction on the DNase molecule.", "content": "The amino acid compositions of various fragments isolated from DNase treated with 2-nitro-5-thiocyanobenzoic acid (NTCB) show peptide bond cleavages to be at Thr14, Ser40, and Ser135. Isolation and characterization of radioactive tryptic and chymotryptic peptides of [14C]cyano-DNase reveal four points of peptide bond cleavage; in addition to Thr14, Ser40, and Ser135, cleavage occurs at the amino end of Ser72. Approximately 2.8 mol of [14C]cyano group are incorporated in the completely inactivated enzyme, in which 0.6 residue of Thr14, 0.8 of Ser40, and approximately 0.3 each of Ser72 and Ser135 are modified. The inactivation by NTCB can also be obtained by reacting the enzyme with a mixture of 5,5'-dithiobis(2-nitrobenzoic acid), KCN, and iodoacetate which generates NTCB. The mixture facilitates the uses of K[14C]N, which is readily incorporated into the enzyme as the [14C]cyano derivative. The reaction of NTCB with serine or threonine resembles that with cysteine.", "contents": "Inactivation of DNase by 2-nitro-5-thiocyanobenzoic acid. II. Serine and threonine are the sites of reaction on the DNase molecule. The amino acid compositions of various fragments isolated from DNase treated with 2-nitro-5-thiocyanobenzoic acid (NTCB) show peptide bond cleavages to be at Thr14, Ser40, and Ser135. Isolation and characterization of radioactive tryptic and chymotryptic peptides of [14C]cyano-DNase reveal four points of peptide bond cleavage; in addition to Thr14, Ser40, and Ser135, cleavage occurs at the amino end of Ser72. Approximately 2.8 mol of [14C]cyano group are incorporated in the completely inactivated enzyme, in which 0.6 residue of Thr14, 0.8 of Ser40, and approximately 0.3 each of Ser72 and Ser135 are modified. The inactivation by NTCB can also be obtained by reacting the enzyme with a mixture of 5,5'-dithiobis(2-nitrobenzoic acid), KCN, and iodoacetate which generates NTCB. The mixture facilitates the uses of K[14C]N, which is readily incorporated into the enzyme as the [14C]cyano derivative. The reaction of NTCB with serine or threonine resembles that with cysteine."}
{"id": "PMID:489556", "title": "Measurement of phenylalanine hydroxylase turnover in cultured hepatoma cells.", "content": "A substantially new method has been developed to measure protein turnover. Its basis is the notion that in labeling experiments a secreted protein can be used to determine the specific radioactivity of the intracellular amino acid precursor pool. To measure protein turnover in the Reuber hepatoma H4 cell line, cultures were labeled with [3H]leucine for specified periods after which phenylalanine hydroxylase was isolated and its leucine specific radioactivity determined. Serum albumin secreted by the cultures was also isolated and used to estimate the leucine precursor pool specific radioactivity. The protein half-life of phenylalanine hydroxylase could them be calculated. Experiments performed at long and short labeling times and with high and low concentrations of leucine in the medium yielded equivalent results. Phenylalanine hydroxylase half-life in the H4 cells was investigated under both normal and hydrocortisone-induced growth conditions. Average half-lives of 7.4 and 8.2 h were found for induced and uninduced cultures, respectively. Although these measured enzyme half-lives were not essentially different, the steady state level of phenylalanine hydroxylase was increased 6.2-fold upon hydrocortisone induction, from 0.076 to 0.47 microgram/10(6) cells. The results demonstrated that hydrocortisone induces phenylalanine hydroxylase in the H4 cells by causing an increase in the rate of enzyme synthesis.", "contents": "Measurement of phenylalanine hydroxylase turnover in cultured hepatoma cells. A substantially new method has been developed to measure protein turnover. Its basis is the notion that in labeling experiments a secreted protein can be used to determine the specific radioactivity of the intracellular amino acid precursor pool. To measure protein turnover in the Reuber hepatoma H4 cell line, cultures were labeled with [3H]leucine for specified periods after which phenylalanine hydroxylase was isolated and its leucine specific radioactivity determined. Serum albumin secreted by the cultures was also isolated and used to estimate the leucine precursor pool specific radioactivity. The protein half-life of phenylalanine hydroxylase could them be calculated. Experiments performed at long and short labeling times and with high and low concentrations of leucine in the medium yielded equivalent results. Phenylalanine hydroxylase half-life in the H4 cells was investigated under both normal and hydrocortisone-induced growth conditions. Average half-lives of 7.4 and 8.2 h were found for induced and uninduced cultures, respectively. Although these measured enzyme half-lives were not essentially different, the steady state level of phenylalanine hydroxylase was increased 6.2-fold upon hydrocortisone induction, from 0.076 to 0.47 microgram/10(6) cells. The results demonstrated that hydrocortisone induces phenylalanine hydroxylase in the H4 cells by causing an increase in the rate of enzyme synthesis."}
{"id": "PMID:489557", "title": "Conformational studies of peptide heart stimulant anthopleurin A. Laser Raman, circular dichroism, fluorescence spectral studies, and Chou-Fasman calculations.", "content": "Sea anemone contain a number of closely related peptide heart stimulants. In the present investigation, the conformation of anthropleurin A from Anthopleura xanthogrammica was investigated by laser Raman, circular dichroism, and fluorescence spectral methods and by the Chou-Fasman method using sequence data. The recent 13C NMR data of the peptide (Norton, R.S., and Norton, T.R. (1979) J. Biol. Chem., in press) provided useful information for the interpretation of the above-mentioned spectral data. The results from these spectral methods suggested that anthropleurin A and the related sea anemone peptides are roughly spherical in shape due to the presence of some beta-bends, possibly due to a beta-pleated sheet region and due to the 3 cystine residues in the peptide which exist in the gauche-gauche-gauche configuration. The sole tyrosine residue is exposed to the solvent, a finding which has now been confirmed by 13C NMR. The laser Raman and fluorescence spectral procedures showed that one or more of the tryptophan residues are buried. Interestingly, the reduction of the native protein with dithioerythritol did not change the spherical shape even in the presence of 5 M guanidine HCl and the carboxymethylcysteine derivative of the peptide was folded even in the presence of the denaturing agent, guanidine HCl.", "contents": "Conformational studies of peptide heart stimulant anthopleurin A. Laser Raman, circular dichroism, fluorescence spectral studies, and Chou-Fasman calculations. Sea anemone contain a number of closely related peptide heart stimulants. In the present investigation, the conformation of anthropleurin A from Anthopleura xanthogrammica was investigated by laser Raman, circular dichroism, and fluorescence spectral methods and by the Chou-Fasman method using sequence data. The recent 13C NMR data of the peptide (Norton, R.S., and Norton, T.R. (1979) J. Biol. Chem., in press) provided useful information for the interpretation of the above-mentioned spectral data. The results from these spectral methods suggested that anthropleurin A and the related sea anemone peptides are roughly spherical in shape due to the presence of some beta-bends, possibly due to a beta-pleated sheet region and due to the 3 cystine residues in the peptide which exist in the gauche-gauche-gauche configuration. The sole tyrosine residue is exposed to the solvent, a finding which has now been confirmed by 13C NMR. The laser Raman and fluorescence spectral procedures showed that one or more of the tryptophan residues are buried. Interestingly, the reduction of the native protein with dithioerythritol did not change the spherical shape even in the presence of 5 M guanidine HCl and the carboxymethylcysteine derivative of the peptide was folded even in the presence of the denaturing agent, guanidine HCl."}
{"id": "PMID:489559", "title": "The in vivo distribution of immunoreactive larger than tetrameric polyadenosine diphosphoribose in histone and non-histone protein fractions of rat liver.", "content": "The macromolecular association of immunoreactive naturally occurring polyadenosine diphosphoribose n greater than 4 with histones and non-histone proteins was determined with the aid of an improved method of extraction of polyadenosine diphosphoribose and a combination of radioimmunoassay and molecular filtration. More than 99% of the naturally occurring polyadenosine diphosphoribose n greater than 4 was present in rat liver in covalent association with non-histone proteins. The chain length of the polymer varied between n = 4 and n = 34. Less than 1% of naturally occurring polyadenosine diphosphoribose n greater than 4 was almost evenly distributed between histone fractions f1, f2a, f2b, and f3. Adenosine diphosphoribose polymers of relatively long chain length were also detected in the histone fractions. The covalent association of polyadenosine diphosphoribose with non-histone proteins was demonstrated by affinity chromatography.", "contents": "The in vivo distribution of immunoreactive larger than tetrameric polyadenosine diphosphoribose in histone and non-histone protein fractions of rat liver. The macromolecular association of immunoreactive naturally occurring polyadenosine diphosphoribose n greater than 4 with histones and non-histone proteins was determined with the aid of an improved method of extraction of polyadenosine diphosphoribose and a combination of radioimmunoassay and molecular filtration. More than 99% of the naturally occurring polyadenosine diphosphoribose n greater than 4 was present in rat liver in covalent association with non-histone proteins. The chain length of the polymer varied between n = 4 and n = 34. Less than 1% of naturally occurring polyadenosine diphosphoribose n greater than 4 was almost evenly distributed between histone fractions f1, f2a, f2b, and f3. Adenosine diphosphoribose polymers of relatively long chain length were also detected in the histone fractions. The covalent association of polyadenosine diphosphoribose with non-histone proteins was demonstrated by affinity chromatography."}
{"id": "PMID:489561", "title": "Random location of nucleosomes on genes for 5 S rRNA.", "content": "The location of nucleosomes on genes for 5 S rRNA in rat liver was determined by the preparation of nucleosome core DNA fragments, hybridization with 5 S rRNA, RNase digestion, and gel electrophoresis. The resulting size distribution of RNA fragments was essentially the same as that found when the experiment was carried out with random DNA fragments.", "contents": "Random location of nucleosomes on genes for 5 S rRNA. The location of nucleosomes on genes for 5 S rRNA in rat liver was determined by the preparation of nucleosome core DNA fragments, hybridization with 5 S rRNA, RNase digestion, and gel electrophoresis. The resulting size distribution of RNA fragments was essentially the same as that found when the experiment was carried out with random DNA fragments."}
{"id": "PMID:489563", "title": "Purification and properties of endo-beta-N-acetylglucosaminidase L from Streptomyces plicatus.", "content": "An enzyme, previously described as endo-beta-N-acetylglucosaminidase L (Tarentino, A.L., and Maley, F. (1974) J. Biol. Chem. 249, 811-817) because of its apparent specificity for Man(GlcNAc)2Asn, has been purified to homogeneity. The enzyme has now been found to hydrolyze (GlcNAc)3 to (GlcNAc)2 plus GlcNAc, and (GlcNAc)4 to 2(GlcNAc)2, at twice the rate observed for Man(GlcNAc)2Asn. Removal of the asparagine from the latter compound reduces the rate of hydrolysis by about 30-fold. Reduction of (GlcNAc)3 to GlcNAc beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc-ol eliminates this compound as a substrate for endo-beta-N-acetylglucosaminidase L. However, the reduction of (GlcNAc)4 does not affect its rate of hydrolysis. Endo-beta-N-acetylglucosaminidase L consists of a single polypeptide chain with a molecular weight of 49,500 +/- 400, which on isoelectric focusing separates into two closely migrating bands; a major with a pI of 4.25 and a minor one with a pI 4.20. Both bands possess similar enzyme activities and amino acid compositions, but differ slightly in their tryptic peptide maps.", "contents": "Purification and properties of endo-beta-N-acetylglucosaminidase L from Streptomyces plicatus. An enzyme, previously described as endo-beta-N-acetylglucosaminidase L (Tarentino, A.L., and Maley, F. (1974) J. Biol. Chem. 249, 811-817) because of its apparent specificity for Man(GlcNAc)2Asn, has been purified to homogeneity. The enzyme has now been found to hydrolyze (GlcNAc)3 to (GlcNAc)2 plus GlcNAc, and (GlcNAc)4 to 2(GlcNAc)2, at twice the rate observed for Man(GlcNAc)2Asn. Removal of the asparagine from the latter compound reduces the rate of hydrolysis by about 30-fold. Reduction of (GlcNAc)3 to GlcNAc beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc-ol eliminates this compound as a substrate for endo-beta-N-acetylglucosaminidase L. However, the reduction of (GlcNAc)4 does not affect its rate of hydrolysis. Endo-beta-N-acetylglucosaminidase L consists of a single polypeptide chain with a molecular weight of 49,500 +/- 400, which on isoelectric focusing separates into two closely migrating bands; a major with a pI of 4.25 and a minor one with a pI 4.20. Both bands possess similar enzyme activities and amino acid compositions, but differ slightly in their tryptic peptide maps."}
{"id": "PMID:489566", "title": "Partial purification and properties of a rat brain phospholipase.", "content": "At least 90% of a membrane-bound phospholipase D was solubilized by extraction of freeze-dried rat brain with 0.8% Miranol H2M and 0.5% cholate. The bulk of base exchange reaction enzymes remained firmly bound to the particulate fraction under these conditions. The phospholipase D specific activity was enriched 240-fold by a purification protocol employing ammonium sulfate precipitation, and both Sepharose 4B and DEAE-cellulose column chromatography. The approximate molecular weight of the partially purified enzyme was calculated to be 200,000 based upon the elution profile from Sepharose 4B and Sephadex G-200 columns. The optimum pH was 6.0, and Km values for phosphatidylcholine and phosphatidylethanolamine were 0.75 mM and 0.91 mM, respectively. The enzyme activity was not dependent on the presence of divalent cation although Ca2+ and Fe2+ showed stimulatory effects.", "contents": "Partial purification and properties of a rat brain phospholipase. At least 90% of a membrane-bound phospholipase D was solubilized by extraction of freeze-dried rat brain with 0.8% Miranol H2M and 0.5% cholate. The bulk of base exchange reaction enzymes remained firmly bound to the particulate fraction under these conditions. The phospholipase D specific activity was enriched 240-fold by a purification protocol employing ammonium sulfate precipitation, and both Sepharose 4B and DEAE-cellulose column chromatography. The approximate molecular weight of the partially purified enzyme was calculated to be 200,000 based upon the elution profile from Sepharose 4B and Sephadex G-200 columns. The optimum pH was 6.0, and Km values for phosphatidylcholine and phosphatidylethanolamine were 0.75 mM and 0.91 mM, respectively. The enzyme activity was not dependent on the presence of divalent cation although Ca2+ and Fe2+ showed stimulatory effects."}
{"id": "PMID:489570", "title": "Steady state and pre-steady state kinetic properties of rat liver selenium-glutathione peroxidase.", "content": "The kinetic properties of partially purified rat liver selenium-glutathione peroxidase were studied under various conditions. Steady state kinetic measurements show sigmoidal saturation curves, parabolic double reciprocal plots, and Hill coefficients greater than unity. Although these kinetic results appear to show cooperative interactions between subunits, they more reflect the presence of several oxidation-reduction forms of the catalytic site. A substrate-induced transition between enzyme forms was evidence by the occurrence of a lag in the attainment of the final steady state velocity under certain preincubation conditions. This hysteretic behavior was evident only when the enzyme was incubated in the absence of reduced glutathione, the donor substrate. Thus, reduced glutathione induces the transition to the fully active form of the enzyme, a slow process requiring about 0.5 min after addition of glutathione, depending on conditions. The length, tau, of the lag period is dependent on the concentrations of enzyme and glutathione, but to a first approximation, this lag period is independent of the concentration of the hydroperoxide acceptor substrate. The lag period is also relatively independent of the nature of the hydroperoxide species. A model for the transition process that is compatible with these observations and with the possible oxidation-reduction properties of the selenium moiety of the enzyme is suggested.", "contents": "Steady state and pre-steady state kinetic properties of rat liver selenium-glutathione peroxidase. The kinetic properties of partially purified rat liver selenium-glutathione peroxidase were studied under various conditions. Steady state kinetic measurements show sigmoidal saturation curves, parabolic double reciprocal plots, and Hill coefficients greater than unity. Although these kinetic results appear to show cooperative interactions between subunits, they more reflect the presence of several oxidation-reduction forms of the catalytic site. A substrate-induced transition between enzyme forms was evidence by the occurrence of a lag in the attainment of the final steady state velocity under certain preincubation conditions. This hysteretic behavior was evident only when the enzyme was incubated in the absence of reduced glutathione, the donor substrate. Thus, reduced glutathione induces the transition to the fully active form of the enzyme, a slow process requiring about 0.5 min after addition of glutathione, depending on conditions. The length, tau, of the lag period is dependent on the concentrations of enzyme and glutathione, but to a first approximation, this lag period is independent of the concentration of the hydroperoxide acceptor substrate. The lag period is also relatively independent of the nature of the hydroperoxide species. A model for the transition process that is compatible with these observations and with the possible oxidation-reduction properties of the selenium moiety of the enzyme is suggested."}
{"id": "PMID:489573", "title": "Membrane microenvironmental changes during activation of human blood platelets by thrombin. A study with a fluorescent probe.", "content": "Membrane microenvironmental changes associated with thrombin-induced platelet activation were followed by fluorescence intensity and polarization studies of 1,6-diphenyl-1,3,5-hexatriene (DPH)-labeled human platelets. The labeling of washed platelets with DPH did not alter platelet intactness and morphology. In response to thrombin, DPH-labeled platelets exhibited reduced serotonin release, yet aggregation was barely inhibited. Shape change induced by thrombin or ADP was indistinguishable in control and in DPH-labeled platelets. During platelet aggregation induced by thrombin, fluorescence intensity increased by about 14%, which may indicate a more hydrophobic exposure of the probe. However, no change in fluorescence was detected during platelet shape change, induced either by thrombin in presence of EDTA or by ADP. Thrombin-activated platelets exhibited an increase in values of fluorescence polarization (P) during the stages of shape change and secretion, which further increased during aggregation. A similar pattern of increase in P values characterized platelet shape changes, caused either by thrombin in the presence of EDTA or by ADP. Changes in individual platelets are discernible from the alterations of the aggregating cells. These results may indicate that platelet activation is accompanied by an increase in rigidity of the membrane lipids. Functionally, the elevated \"microviscosity\" may reflect a primary role of membrane lipids in modulating the process of platelet activation or secondary transitions in lipids due to membrane events mediated by proteins.", "contents": "Membrane microenvironmental changes during activation of human blood platelets by thrombin. A study with a fluorescent probe. Membrane microenvironmental changes associated with thrombin-induced platelet activation were followed by fluorescence intensity and polarization studies of 1,6-diphenyl-1,3,5-hexatriene (DPH)-labeled human platelets. The labeling of washed platelets with DPH did not alter platelet intactness and morphology. In response to thrombin, DPH-labeled platelets exhibited reduced serotonin release, yet aggregation was barely inhibited. Shape change induced by thrombin or ADP was indistinguishable in control and in DPH-labeled platelets. During platelet aggregation induced by thrombin, fluorescence intensity increased by about 14%, which may indicate a more hydrophobic exposure of the probe. However, no change in fluorescence was detected during platelet shape change, induced either by thrombin in presence of EDTA or by ADP. Thrombin-activated platelets exhibited an increase in values of fluorescence polarization (P) during the stages of shape change and secretion, which further increased during aggregation. A similar pattern of increase in P values characterized platelet shape changes, caused either by thrombin in the presence of EDTA or by ADP. Changes in individual platelets are discernible from the alterations of the aggregating cells. These results may indicate that platelet activation is accompanied by an increase in rigidity of the membrane lipids. Functionally, the elevated \"microviscosity\" may reflect a primary role of membrane lipids in modulating the process of platelet activation or secondary transitions in lipids due to membrane events mediated by proteins."}
{"id": "PMID:489575", "title": "Determination of the turn-off reaction for the hormone-activated adenylate cyclase.", "content": "Previous work suggested that hormonal activation of adenylate cyclase involves the introduction of GTP to the regulatory site, and subsequent hydrolysis of the bound GTP terminates the activation. In many tissues the turn-off GTPase reaction cannot be readily measured because of a high background of nonspecific GTP hydrolysis. To circumvent this problem a general assay for the turn-off reaction has now been developed. The adenylate cyclase is first activated by hormone and GTP and the introduction of GTP is then stopped either by addition of an excess of guanosine 5'-O-(2-thiodiphosphate) (GDP beta S) or by addition of a receptor blocking agent. The decay of adenylate cyclase activity brought on by these inhibitors is used to calculate the rate constant of the turn-off reaction. In turkey erythrocyte and rat parotid membranes the rate constant of the decay process as determined with GDP beta S is similar to that determined with the beta-adrenergic blocker propranolol. The rate constants (min-1 at 30 degrees C) for various adenylate cyclase preparations are 10 for turkey erythrocyte, 7.5 for rat parotid, and 6.2 for the rat liver enzyme. The finding of similar rate constants in the various preparations indicates that GTP hydrolysis at the regulatory site is a general mechanism for terminating the activation of adenylate cyclase.", "contents": "Determination of the turn-off reaction for the hormone-activated adenylate cyclase. Previous work suggested that hormonal activation of adenylate cyclase involves the introduction of GTP to the regulatory site, and subsequent hydrolysis of the bound GTP terminates the activation. In many tissues the turn-off GTPase reaction cannot be readily measured because of a high background of nonspecific GTP hydrolysis. To circumvent this problem a general assay for the turn-off reaction has now been developed. The adenylate cyclase is first activated by hormone and GTP and the introduction of GTP is then stopped either by addition of an excess of guanosine 5'-O-(2-thiodiphosphate) (GDP beta S) or by addition of a receptor blocking agent. The decay of adenylate cyclase activity brought on by these inhibitors is used to calculate the rate constant of the turn-off reaction. In turkey erythrocyte and rat parotid membranes the rate constant of the decay process as determined with GDP beta S is similar to that determined with the beta-adrenergic blocker propranolol. The rate constants (min-1 at 30 degrees C) for various adenylate cyclase preparations are 10 for turkey erythrocyte, 7.5 for rat parotid, and 6.2 for the rat liver enzyme. The finding of similar rate constants in the various preparations indicates that GTP hydrolysis at the regulatory site is a general mechanism for terminating the activation of adenylate cyclase."}
{"id": "PMID:489576", "title": "Isolation of a protein from the plasma membrane of adrenal medulla which binds to secretory vesicles.", "content": "Solubilized proteins of the plasma membrane of bovine adrenal medulla were fractionated on the basis of their affinity for secretory vesicles. The isolation procedure included preparation of a highly purified fraction of plasma membranes, its solubilization in detergent, and application to a column prepared from glutaraldehyde-fixed chromaffin granules. Using this technique, one major polypeptide (80% of the material bound) was isolated. This protein has been shown to originate from the plasma membrane and has no affinity for fixed bovine adrenal medullary mitochondria or lysosomes. It is eluted most effectively by low pH (3.0) and can be rebound and re-eluted from fixed secretory granules. In sodium dodecyl sulfate and beta-mercaptoethanol it has an apparent molecular weight of 51,000. In addition, two minor components, comprising about 20% of the material bound were detected having apparent molecular weights in sodium dodecyl sulfate of 14,000 and 62,000. It is suggested that such a molecule could function as a plasma membrane-located receptor for chromaffin granules during the secretory process.", "contents": "Isolation of a protein from the plasma membrane of adrenal medulla which binds to secretory vesicles. Solubilized proteins of the plasma membrane of bovine adrenal medulla were fractionated on the basis of their affinity for secretory vesicles. The isolation procedure included preparation of a highly purified fraction of plasma membranes, its solubilization in detergent, and application to a column prepared from glutaraldehyde-fixed chromaffin granules. Using this technique, one major polypeptide (80% of the material bound) was isolated. This protein has been shown to originate from the plasma membrane and has no affinity for fixed bovine adrenal medullary mitochondria or lysosomes. It is eluted most effectively by low pH (3.0) and can be rebound and re-eluted from fixed secretory granules. In sodium dodecyl sulfate and beta-mercaptoethanol it has an apparent molecular weight of 51,000. In addition, two minor components, comprising about 20% of the material bound were detected having apparent molecular weights in sodium dodecyl sulfate of 14,000 and 62,000. It is suggested that such a molecule could function as a plasma membrane-located receptor for chromaffin granules during the secretory process."}